bluuebunny/crossref_metadata_2025 huggingface dataset:
Cardiac GRK2 Protein Levels Show Sexual Dimorphism during Aging and Are Regulated by Ovarian Hormones
Authors: Arcones Alba C. , Martínez-Cignoni Melanie Raquel , Vila-Bedmar Rocío , Yáñez Claudia , Lladó Isabel , Proenza Ana M. , Mayor Federico , Murga Cristina
Published: March 2021

Cardiovascular disease (CVD) risk shows a clear sexual dimorphism with age, with a lower incidence in young women compared to age-matched men. However, this protection is lost after menopause. We demonstrate that sex-biased sensitivity to the development of CVD with age runs in parallel with changes in G protein-coupled receptor kinase 2 (GRK2) protein levels in the murine heart and that mitochondrial fusion markers, related to mitochondrial functionality and cardiac health, inversely correlate with GRK2. Young female mice display lower amounts of cardiac GRK2 protein compared to age-matched males, whereas GRK2 is upregulated with age specifically in female hearts. Such an increase in GRK2 seems to be specific to the cardiac muscle since a different pattern is found in the skeletal muscles of aging females. Changes in the cardiac GRK2 protein do not seem to rely on transcriptional modulation since adrbk1 mRNA does not change with age and no differences are found between sexes. Global changes in proteasomal or autophagic machinery (known regulators of GRK2 dosage) do not seem to correlate with the observed GRK2 dynamics. Interestingly, cardiac GRK2 upregulation in aging females is recapitulated by ovariectomy and can be partially reversed by estrogen supplementation, while this does not occur in the skeletal muscle. Our data indicate an unforeseen role for ovarian hormones in the regulation of GRK2 protein levels in the cardiac muscle which correlates with the sex-dependent dynamics of CVD risk, and might have interesting therapeutic applications, particularly for post-menopausal women.
Perspectives of Black/African American and Hispanic Parents and Children Living in Under-Resourced Communities Regarding Factors That Influence Food Choices and Decisions: A Qualitative Investigation
Authors: Thompson Debbe , Callender Chishinga , Velazquez Denisse , Adera Meheret , Dave Jayna , Olvera Norma , Chen Tzu-An , Goldsworthy Natalie
Published: March 2021

Families living in under-resourced communities are at risk of obesity and obesity-related chronic diseases. To develop effective interventions, it is important to identify parent and child perspectives of factors that influence food-related choices and decisions. This paper reports qualitative findings from a larger mixed method study investigating this topic. Hybrid thematic analysis was used to code and analyze the interviews. Family-generated photographs of factors influencing food choices were discussed during the interviews. Qualitative findings were organized by the socio-ecological model. Verbatim quotes and photographs were used to support themes. Thirty-six interviews were conducted (18 parents, 18 children). Findings from parents revealed personal (e.g., culture, beliefs, time), family (e.g., mother, child, father, health, finances, cohesiveness), environmental (e.g., availability, convenience, cost), and other (e.g., school food) factors influenced food choices. Similarly, child-reported influences were personal (e.g., preferences, beliefs, taste), family (e.g., mother, family encouragement, father, family time), social (e.g., school, friends), environmental (e.g., availability), and other (e.g., media, sports). The socio-ecological model provided a useful framework for identifying factors that influence food choices and decisions of families living in under-resourced communities. A deeper understanding of these factors could enhance both responsiveness and effectiveness of interventions to enhance diet and reduce obesity risk in families living in under-resourced communities.
Crisis Management in Higher Education in the Time of Covid-19: The Case of Actor Training
Authors: McNamara Anna
Published: March 2021

The impact of Covid-19 placed Higher Education leadership in a state of crisis management, where decision making had to be swift and impactful. This research draws on ethea of mindfulness, actor training techniques, referencing high-reliability organisations (HRO). Interviews conducted by the author with three leaders of actor training conservatoires in Higher Education institutions in Australia, the UK and the USA reflect on crisis management actions taken in response to the impact of Covid-19 on their sector, from which high-frequency words are identified and grouped thematically. Reflecting on these high-frequency words and the thematic grouping, a model of mindful leadership is proposed as a positive tool that may enable those in leadership to recognise and respond efficiently to wider structural frailties within Higher Education, with reference to the capacity of leaders to operate with increased mindfulness, enabling a more resilient organisation that unlocks the locus of control.
Backup Capacity Planning Considering Short-Term Variability of Renewable Energy Resources in a Power System
Authors: Lee Deukyoung , Lee Dongjun , Jang Hanhwi , Joo Sung-Kwan
Published: March 2021

Increasing renewable energy penetration rate in a power grid leads to an increase in the variability of the generated energy, which increases the system integration cost. To handle the output variations in the generation, it is necessary to secure sufficient flexible resources, such as energy storage units. Flexible resources can adjust the output quickly, which helps to increase the system flexibility. However, the electricity generation cost of the flexible resources is usually high. Because the renewable energy expansion policy is being implemented worldwide, it is necessary to evaluate the ability to manage the short-term variations of the renewable energy outputs to obtain a cost-effective long-term plan. In this study, the variability of renewable energy in Korea over the past five years was analyzed. Additionally, the backup capacity is determined to manage the variability of renewable energy output. The backup capacity is affected by system flexibility. In general, increasing system flexibility decreases the backup capacity and increases the total electricity production cost. In this study, a backup capacity planning method is proposed considering the short-term variability of renewable energy output and flexibility deficit in a power system. The numerical results illustrated the effectiveness of the proposed backup capacity planning method.
Heart Images on Food Labels: A Health Claim or Not?
Authors: Miklavec Krista , Hribar Maša , Kušar Anita , Pravst Igor
Published: March 2021

Health claims on food labels are used by food manufacturers to inform consumers about the health effects of a product, and such claims can have notable effects on consumer preferences. According to regulatory definitions, health claims can be either worded or presented as images, but it is not clear under which conditions an image on a food label should be considered a health claim. This question has important practical implications, as the use of health claims is strictly regulated. The objective of this study was to determine how commonly images of the heart are used on food labels, and to investigate consumers’ perceptions of products labelled with heart images, using different degrees of health relationships. Both a food supply study (N = 10,573 foods) and experiments with consumers (N = 1000) were performed in Slovenia. The use of heart imagery on food products was very common (9%). The consumer study was conducted using a web panel. Structure of the study population was comparable with Slovenian adult population (18–65 years), according to gender and age. The questionnaire was split into conjoint analysis with constructed elements, a choice-based task with real-life elements and a consumers’ association task. The experiments showed that a heart image as part of the brand name itself—without an additional (worded) health claim—did not cause most consumers to relate it to health. However, consumers tended to strongly relate an image of the heart as part of a brand with health benefits, where the image was accompanied by a worded health claim or if the heart image was designed specifically to imply health benefits. We can conclude that the use of heart images was very common on food products, but references to health were less common. Without a health-related context, heart images could not be considered as a health claim.
A Model Predictive Control for the Dynamical Forecast of Operating Reserves in Frequency Regulation Services
Authors: Nikolaidis Pavlos , Partaourides Harris
Published: March 2021

The intermittent and uncontrollable power output from the ever-increasing renewable energy sources, require large amounts of operating reserves to retain the system frequency within its nominal range. Based on day-ahead load forecasts, many research works have proposed conventional and stochastic approaches to define their optimum margins for reliability enhancement at reasonable production cost. In this work, we aim at delivering real-time load forecasting to lower the operating-reserve requirements based on intra-hour weather update predictors. Based on critical predictors and their historical data, we train an artificial model that is able to forecast the load ahead with great accuracy. This is a feed-forward neural network with two hidden layers, which performs real-time forecasts with the aid of a predictive model control developed to update the recommendations intra-hourly and, assessing their impact and its significance on the output target, it corrects the imposed deviations. Performing daily simulations for an annual time-horizon, we observe that significant improvements exist in terms of decreased operating reserve requirements to regulate the violated frequency. In fact, these improvements can exceed 80% during specific months of winter when compared with robust formulations in isolated power systems.
Effects of the Small-Sided Soccer Games on Blood Pressure in Untrained Hypertensive Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Clemente Filipe Manuel , Ramirez-Campillo Rodrigo , Sarmento Hugo
Published: March 2021

This systematic review with meta-analysis was conducted to assess the effects of small-sided games (SSGs)-based programs on the systolic and diastolic blood pressure of untrained hypertensive adults. The data sources utilized were Web of Science, Scopus, SPORTDiscus, and PubMed. The eligibility criteria were: (i) randomized controlled trials including a control group and an intervention group exclusively using soccer SSGs; (ii) intervention and control groups including an untrained hypertensive adult population; (iii) articles written in English; and (iv) only full-text and original articles. The database search initially identified 241 titles. From those, five articles were eligible for the systematic review and meta-analysis. The included randomized controlled studies involved five individual experimental groups and 88 participants, and 68 participants in the five control groups. The results showed a large and beneficial effect of SSG on systolic (ES = 1.69; 95% CI = 0.71 to 2.66; p = 0.001; I2 = 85.2%; Egger’s test p = 0.101) and diastolic blood pressure (ES = 2.25; 95% CI = 1.44 to 3.06; p < 0.001; I2 = 74.8%; Egger’s test p = 0.118) when compared to the control groups. The findings of the current systematic review and meta-analysis revealed consistent beneficial effects of recreational soccer SSGs on untrained men and women from the hypertensive population, although high levels of heterogeneity.
Effects of Tooth Brushing Training, Based on Augmented Reality Using a Smart Toothbrush, on Oral Hygiene Care among People with Intellectual Disability in Korea
Authors: Jeon Byoungjin , Oh Jinseok , Son Sungmin
Published: March 2021

(1) Purpose: In modern society, augmented reality (AR)-based training using a smart device has emerged as a means of resolving problems with training. Thus, this feasibility study aimed to identify the effects of tooth-brushing training, based on AR using a smart toothbrush, on oral hygiene care among people with an intellectual disability in Korea. (2) Methods: Thirty people with an intellectual disability, residing in a residential care facility, were selected. Tooth-brushing training based on AR, using a smart toothbrush, was applied in the experimental group (n = 15), and training using visual material was applied in the control group (n = 15). As an assessment of oral hygiene care, the changes in tooth-brushing performance and oral hygiene were measured. (3) Results: There were significant differences in all results after training between the two groups. (4) Conclusions: Tooth-brushing training based on AR using a smart toothbrush is more effective than training using visual material on oral hygiene care among the subjects of this feasibility study. Thus, training based on AR using a smart toothbrush could be applied to people with intellectual disabilities residing in residential care facilities as an individual tool for tooth-brushing training.
Biological Control of the Raspberry Eriophyoid Mite Phyllocoptes gracilis Using Entomopathogenic Fungi
Authors: Minguely Camille , Norgrove Lindsey , Burren Alexander , Christ Bastien
Published: March 2021

There is an urgent need to develop biological control methods against the eriophyoid mite, Phyllocoptes gracilis, which causes significant losses in organic raspberry production in Europe. The use of entomopathogenic fungi (EF) is a sustainable alternative to conventional chemical pesticides, reducing the risks of pesticide resistance and other negative environmental impacts of agriculture. The objective of this study was to assess the pathogenicity of three strains of EF, two of Beauveria bassiana and one of Metarhizium anisopliae, on P. gracilis under laboratory conditions. Fungal spore suspensions (1 × 107 spores per mL) were sprayed on detached leaves infested with P. gracilis. Treated mites were kept under controlled conditions (25 ± 3 °C, 72 ± 10% relative humidity and photoperiod of 16:8 (light/dark)) and mite mortality was assessed three, five and seven days after inoculation. At all three measurement points (days after inoculation), the mortality of P. gracilis was highest for B. bassiana strain BB 1.1 and M. anisopliae strain MA 10.1. Our data demonstrate that EFs are promising candidates for the development of biological control agents against P. gracilis in raspberry crops.
A Sustainable Slit Jet FTIR Spectrometer for Hydrate Complexes and Beyond
Authors: Gottschalk Hannes C. , Fischer Taija L. , Meyer Volker , Hildebrandt Reinhard , Schmitt Ulrich , Suhm Martin A.
Published: March 2021

Fourier transform infrared (FTIR) absorption spectroscopy of cold molecules and clusters in supersonic slit jet expansions complements and extends more sensitive action spectroscopy techniques and provides important reference data for the latter. We describe how its major drawback, large substance and carrier gas consumption, can be alleviated by one to two orders of magnitude via direct and continuous recycling of the gas mixture. This is achieved by a combination of dry rotary lobe and screw pump compression. The signal-to-noise ratio is boosted by the established buffered giant gas pulse technique with full interferogram synchronization. The buildup of water impurities typically limits the recycling gain, but is turned into a feature for the study of hydrate complexes of volatile molecules. Continuous operation with a single gas filling over several days becomes practical and useful. Decadic absorbances in the low ppm range are detectable and the mid infrared range can be recorded simultaneously with the near infrared. The less straightforward hydration number assignment of spectral features in direct absorption spectroscopy is supported by a gradual water buildup at a rate of less than 0.5 mg/h. A recent reassignment proposal for the water dimer OH stretching spectrum is refuted and vibrational spectra of vacuum-isolated 18O-water clusters are presented for the first time. Methanol docking on asymmetric ketones is used to illustrate the advantages and limitations of the recycling concept. Previous assignments of the hydrate complex of 1-phenylethanol are confirmed. Additional features of the setup await testing and refinement, but the recycling technique already substantially widens the applicability of direct absorption spectroscopy of neutral molecular clusters. It may be attractive for other high-throughput jet spectrometers.
A Comparison of the Effect of Strength Training on Cycling Performance between Men and Women
Authors: Vikmoen Olav , Rønnestad Bent R.
Published: March 2021

During the last decade numerous review articles have been published on how concurrent strength and endurance training affect cycling performance. However, none of these have reviewed if there are any sex differences in the effects of concurrent training on cycling performance, and most research in this area has been performed with male cyclists. Thus, the aim of the current paper is to review the scientific literature on the effect of concurrent training on cycling performance in male and female cyclists with a special emphasis on potential sex differences. The results indicate that both male and female cyclists experience a similar beneficial effect from concurrent training on cycling performance and its physiological determinants compared to normal endurance training only. Some data indicate that women have a larger effect on cycling economy, but more studies are needed to explore this further. Furthermore, the adaptations to strength training thought to be responsible for the beneficial effects on cycling performance seem to be very similar between men and women. Interestingly, increased muscle cross-sectional area in the main locomotor muscles seems to be an important adaptation for improved performance, and, contrary to popular belief, cyclists should aim for increased muscle cross-sectional area when adding strength training to their normal training. We conclude that both male and female cyclists can improve their cycling performance by adding strength training to their normal training.
Mold and Yeast-Like Fungi in the Seaside Air of the Gulf of Gdańsk (Southern Baltic) after an Emergency Disposal of Raw Sewage
Authors: Michalska Małgorzata , Kurpas Monika , Zorena Katarzyna , Wąż Piotr , Marks Roman
Published: March 2021

The aim of this study was to determine the correlation between the meteorological factors and the number of molds and yeast-like fungi in the air in the five coastal towns in the years 2014–2017, and in 2018, after emergency disposal of raw sewage to the Gdańsk Gulf. In the years 2014–2018, a total number of 88 air samples were collected in duplicate in the five coastal towns of Hel, Puck, Gdynia, Sopot, and Gdańsk-Brzeźno. After the application of the (PCA) analysis, this demonstrated that the first principal component (PC1) had a positive correlation with the water temperature, wind speed, air temperature, and relative humidity. The second principal component (PC2) had a positive correlation with the relative humidity, wind speed, wind direction, and air temperature. In 2018, potentially pathogenic mold and yeast-like fungi (Candida albicans, Stachybotrys chartarum complex, Aspergillus section Fumigati) were detected in the seaside air. While the detected species were not observed in the years 2014–2017. We suggest that it is advisable to inform residents about the potential health risk in the event of raw sewage disposal into the water. Moreover, in wastewater treatment plants, tighter measures, including wastewater disinfection, should be introduced.
Dexamethasone Creates a Suppressive Microenvironment and Promotes Aspergillus fumigatus Invasion in a Human 3D Epithelial/Immune Respiratory Model
Authors: Luvanda Maureen K. , Posch Wilfried , Noureen Asma , Lafon Eliott , Zaderer Viktoria , Lass-Flörl Cornelia , Wilflingseder Doris
Published: March 2021

Lung immunity and susceptibility to infections is subject to interactions between the epithelial layer and immune cells residing in the pulmonary space. Aspergillus (A.) fumigatus, the most prevalent pathogenic fungus, affects both upper and lower respiratory tracts of immunocompromised hosts. Several reports implicate corticosteroids as a major risk factor due to their anti-inflammatory and immunosuppressive effects, which are exacerbated by long-term treatment regimens. Here we demonstrate for the first time the influence of dexamethasone when it comes to germination and hyphae formation of A. fumigatus in the presence of macrophages within a highly differentiated air–liquid interphase (ALI) epithelial/immune lung model. We illustrate suppressed mucus production within the highly differentiated 3D respiratory model as well as significantly decreased cilia beat frequencies by dexamethasone treatment. This goes along with corticosteroid-mediated macrophage M2 polarization within the epithelial/immune microenvironment. Therefore, we here showed that corticosteroids promote enhanced fungal growth and invasion A. fumigatus by creating a suppressive environment affecting both epithelial as well as immune cells.
Mining Open Government Data for Business Intelligence Using Data Visualization: A Two-Industry Case Study
Authors: Gottfried Anne , Hartmann Caroline , Yates Donald
Published: March 2021

The business intelligence (BI) market has grown at a tremendous rate in the past decade due to technological advancements, big data and the availability of open source content. Despite this growth, the use of open government data (OGD) as a source of information is very limited among the private sector due to a lack of knowledge as to its benefits. Scant evidence on the use of OGD by private organizations suggests that it can lead to the creation of innovative ideas as well as assist in making better informed decisions. Given the benefits but lack of use of OGD to generate business intelligence, we extend research in this area by exploring how OGD can be used to generate business intelligence for the identification of market opportunities and strategy formulation; an area of research that is still in its infancy. Using a two-industry case study approach (footwear and lumber), we use latent Dirichlet allocation (LDA) topic modeling to extract emerging topics in these two industries from OGD, and a data visualization tool (pyLDAVis) to visualize the topics in order to interpret and transform the data into business intelligence. Additionally, we perform an environmental scanning of the environment for the two industries to validate the usability of the information obtained. The results provide evidence that OGD can be a valuable source of information for generating business intelligence and demonstrate how topic modeling and visualization tools can assist organizations in extracting and analyzing information for the identification of market opportunities.
Cement-Matrix Composites Using CFRP Waste: A Circular Economy Perspective Using Industrial Symbiosis
Authors: Vitale Pierluca , Napolitano Rosanna , Colella Francesco , Menna Costantino , Asprone Domenico
Published: March 2021

This study aims to provide a mitigation strategy for reducing the economic and environmental impacts of carbon fiber wastes deriving from automotive industry. Recycling and reuse in the construction industry is proposed, according to an industrial symbiosis within a circular economy perspective. Specifically, the process consists of repurposing carbon fiber reinforced polymer (CFRP) scraps/waste into new cement-matrix composites, for which the resulting benefits, in terms of mechanical and environmental performance, are herein described. An experimental campaign, starting with a specific heat treatment of CFRP sheets and an accurate dimensional distribution analysis of the short carbon fibers, is presented. The influence of the fiber content and length on both the workability and the mechanical performance of cement-based carbon fiber reinforced mortars is also evaluated. A reduced amount of either sand or cement (up to 8% and 12.8% in volume, respectively) is also considered in the mix design of the fiber reinforced mortars and derives from the substitution of the sand or binder with an equivalent volume of CFRP fibers. The results show a satisfactory increase in compressive and flexural strength in the range 10–18% for the samples characterized by a volume fraction of fibers of approximately 4% and having a 2–5 mm length. Finally, a life cycle assessment (LCA, 14040/14044) was carried out to quantify the environmental burden reductions associated with the implementation of the proposed symbiotic scheme.
The Convergence Rate of High-Dimensional Sample Quantiles for φ-Mixing Observation Sequences
Authors: Peng Ling , Han Dong
Published: March 2021

In this paper, we obtain the convergence rate for the high-dimensional sample quantiles with the φ-mixing dependent sequence. The resulting convergence rate is shown to be faster than that obtained by the Hoeffding-type inequalities. Moreover, the convergence rate of the high-dimensional sample quantiles for the observation sequence taking discrete values is also provided.
Impact of Lactate Clearance on Early Outcomes in Pediatric ECMO Patients
Authors: Merkle-Storms Julia , Djordjevic Ilija , Weber Carolyn , Avgeridou Soi , Krasivskyi Ihor , Gaisendrees Christopher , Mader Navid , Kuhn-Régnier Ferdinand , Kröner Axel , Bennink Gerardus , Sabashnikov Anton , Trieschmann Uwe , Wahlers Thorsten , Menzel Christoph
Published: March 2021

Background and Objectives: Pediatric extracorporeal membrane oxygenation (ECMO) support is often the ultimate therapy for neonatal and pediatric patients with congenital heart defects after cardiac surgery. The impact of lactate clearance in pediatric patients during ECMO therapy on outcomes has been analyzed. Materials andMethods: We retrospectively analyzed data from 41 pediatric vaECMO patients between January 2006 and December 2016. Blood lactate and lactate clearance have been recorded prior to ECMO implantation and 3, 6, 9 and 12 h after ECMO start. Receiver operating characteristic (ROC) analysis was used to identify cut-off levels for lactate clearance. Results: Lactate levels prior to ECMO therapy (9.8 mmol/L vs. 13.5 mmol/L; p = 0.07) and peak lactate levels during ECMO support (10.4 mmol/L vs. 14.7 mmol/L; p = 0.07) were similar between survivors and nonsurvivors. Areas under the curve (AUC) of lactate clearance at 3, 9 h and 12 h after ECMO start were significantly predictive for mortality (p = 0.017, p = 0.049 and p = 0.006, respectively). Cut-off values of lactate clearance were 3.8%, 51% and 56%. Duration of ECMO support and respiratory ventilation was significantly longer in survivors than in nonsurvivors (p = 0.01 and p < 0.001, respectively). Conclusions: Dynamic recording of lactate clearance after ECMO start is a valuable tool to assess outcomes and effectiveness of ECMO application. Poor lactate clearance during ECMO therapy in pediatric patients is a significant marker for higher mortality.
Effects of Vitamin and Amino Acid-Enriched Hyaluronic Acid Gel on the Healing of Oral Mucosa: In Vivo and In Vitro Study
Authors: Canciani Elena , Sirello Riccardo , Pellegrini Gaia , Henin Dolaji , Perrotta Mariachiara , Toma Marilisa , Khomchyna Nataliya , Dellavia Claudia
Published: March 2021

Background and Objectives: Wound healing is a dynamic process that can be compromised in patients with chronic and metabolic conditions or unhealthy lifestyles. Numerous medical substances designed for topical use, charged with compounds that promote the healing process, have been developed to improve wound healing, especially in compromised subjects. The present study aimed to extend our understanding of the in vivo effects of a hyaluronic acid gel charged with amino acids (HAplus gel, Aminogam gel® Errekappa Euroterapici spa, Milan, Italy) and study the in vitro effects of the same gel charged with additional substances in an attempt to optimize its formulation. Materials and Methods: In a randomized controlled split-mouth clinical and histological trial, HAplus gel was tested on the gingival tissue of the lower third molar post-extraction socket. The gingiva was collected at the time of extraction (T0) and ten days after the extraction (T1) to be histologically analyzed. During the second stage of the study, culture media with HAplus gel and vitamin C and E at different concentrations (TEST) were tested on human gingival fibroblasts and compared to the HAplus-enriched medium (HA-Control). Results: Histological and immunohistochemical analysis of collected gingiva showed higher microvascular density and collagen fibers organized in closely packed and well-oriented bundles in sites treated with HAplus gel. In the in vitro study, all TEST groups showed an increased viability from 24 h to 48 h. After 24 h, the viability percentage in all experimental groups was below 100% of the HA-Control, demonstrating a mild toxicity. After 48 h from seeding, the TEST groups’ viability grew significantly compared to HA-Control. Conclusions: These encouraging preliminary results suggest that the use of HAplus gel enriched with vitamins C and E may be beneficial in patients with conditions that impair soft tissue healing.
Comparative Genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis Strains Isolated from a Single Walnut Host Tree
Authors: Fernandes Camila , Martins Leonor , Teixeira Miguel , Blom Jochen , Pothier Joël F. , Fonseca Nuno A. , Tavares Fernando
Published: March 2021

The recent report of distinct Xanthomonas lineages of Xanthomonas arboricola pv. juglandis and Xanthomonas euroxanthea within the same walnut tree revealed that this consortium of walnut-associated Xanthomonas includes both pathogenic and nonpathogenic strains. As the implications of this co-colonization are still poorly understood, in order to unveil niche-specific adaptations, the genomes of three X. euroxanthea strains (CPBF 367, CPBF 424T, and CPBF 426) and of an X. arboricola pv. juglandis strain (CPBF 427) isolated from a single walnut tree in Loures (Portugal) were sequenced with two different technologies, Illumina and Nanopore, to provide consistent single scaffold chromosomal sequences. General genomic features showed that CPBF 427 has a genome similar to other X. arboricola pv. juglandis strains, regarding its size, number, and content of CDSs, while X. euroxanthea strains show a reduction regarding these features comparatively to X. arboricola pv. juglandis strains. Whole genome comparisons revealed remarkable genomic differences between X. arboricola pv. juglandis and X. euroxanthea strains, which translates into different pathogenicity and virulence features, namely regarding type 3 secretion system and its effectors and other secretory systems, chemotaxis-related proteins, and extracellular enzymes. Altogether, the distinct genomic repertoire of X. euroxanthea may be particularly useful to address pathogenicity emergence and evolution in walnut-associated Xanthomonas.
Functional Analysis of an Acyltransferase-Like Domain from Polyunsaturated Fatty Acid Synthase in Thraustochytrium
Authors: Almendáriz-Palacios Carla , Meesapyodsuk Dauenpen , Qiu Xiao
Published: March 2021

Biosynthesis of very long chain polyunsaturated fatty acids (VLCPUFA) such as docosahexaenoic acid (DHA, 22:6-4,7,10,13,16,19) and docosapentaenoic acid (DPA, 22:5-4,7,10,13,16) in protist Thraustochytrium is catalyzed by a polyunsaturated fatty acids (PUFA) synthase comprising three large subunits, each with multiple catalytic domains. This study used complementation test, in vitro assays, and functional expression to characterize an acyltransferase (AT)-like domain in Subunit-B of a PUFA synthase from Thraustochytrium. Complementation test in Escherichia coli showed that the AT-like domain could not restore the growth phenotype of a temperature-sensitive mutant (∆fabDts) defective in malonyl-CoA:ACP transacylase activity. In vitro assays showed that the AT-like domain possessed thioesterase activity towards a few acyl-CoAs tested where docosahexaenoyl-CoA (DHA-CoA) was the preferred substrate. Expression of this domain in an E. coli mutant (∆fadD) defective in acyl-CoA synthetase activity resulted in the increased accumulation of free fatty acids. Site-directed mutagenesis showed that the substitution of two putative active site residues, serine at 96 (S96) and histidine at 220 (H220), in the AT-like domain significantly reduced its activity towards DHA-CoA and accumulation of free fatty acids in the ∆fadD mutant. These results indicate that the AT-like domain of the PUFA synthase does not function as a malonyl-CoA:ACP transacylase, rather it functions as a thioesterase. It might catalyze the last step of the VLCPUFA biosynthesis by releasing freshly synthesized VLCPUFAs attached to ACP domains of the PUFA synthase in Thraustochytrium.
Discovery of Staphylococcus aureus Adhesion Inhibitors by Automated Imaging and Their Characterization in a Mouse Model of Persistent Nasal Colonization
Authors: Fernandes de Oliveira Liliane Maria , Steindorff Marina , Darisipudi Murthy N. , Mrochen Daniel M. , Trübe Patricia , Bröker Barbara M. , Brönstrup Mark , Tegge Werner , Holtfreter Silva
Published: March 2021

Due to increasing mupirocin resistance, alternatives for Staphylococcus aureus nasal decolonization are urgently needed. Adhesion inhibitors are promising new preventive agents that may be less prone to induce resistance, as they do not interfere with the viability of S. aureus and therefore exert less selection pressure. We identified promising adhesion inhibitors by screening a library of 4208 compounds for their capacity to inhibit S. aureus adhesion to A-549 epithelial cells in vitro in a novel automated, imaging-based assay. The assay quantified DAPI-stained nuclei of the host cell; attached bacteria were stained with an anti-teichoic acid antibody. The most promising candidate, aurintricarboxylic acid (ATA), was evaluated in a novel persistent S. aureus nasal colonization model using a mouse-adapted S. aureus strain. Colonized mice were treated intranasally over 7 days with ATA using a wide dose range (0.5–10%). Mupirocin completely eliminated the bacteria from the nose within three days of treatment. In contrast, even high concentrations of ATA failed to eradicate the bacteria. To conclude, our imaging-based assay and the persistent colonization model provide excellent tools to identify and validate new drug candidates against S. aureus nasal colonization. However, our first tested candidate ATA failed to induce S. aureus decolonization.
Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species
Authors: Arraki Kamel , Totoson Perle , Decendit Alain , Zedet Andy , Maroilley Justine , Badoc Alain , Demougeot Céline , Girard Corine
Published: March 2021

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1–2), together with three other stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The arginase inhibitory activity of compounds 1–7 was evaluated through an in vitro arginase inhibitory assay using purified liver bovine arginase. As a result, five compounds (1, 4–7) showed significant inhibition of arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural arginase inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.
Thermoplastic Cellulose-Based Compound for Additive Manufacturing
Authors: Immonen Kirsi , Willberg-Keyriläinen Pia , Ropponen Jarmo , Nurmela Asta , Metsä-Kortelainen Sini , Kaukoniemi Otto-Ville , Kangas Heli
Published: March 2021

The increasing environmental awareness is driving towards novel sustainable high-performance materials applicable for future manufacturing technologies like additive manufacturing (AM). Cellulose is abundantly available renewable and sustainable raw material. This work focused on studying the properties of thermoplastic cellulose-based composites and their properties using injection molding and 3D printing of granules. The aim was to maximize the cellulose content in composites. Different compounds were prepared using cellulose acetate propionate (CAP) and commercial cellulose acetate propionate with plasticizer (CP) as polymer matrices, microcellulose (mc) and novel cellulose-ester additives; cellulose octanoate (C8) and cellulose palmitate (C16). The performance of compounds was compared to a commercial poly(lactic acid)-based cellulose fiber containing composite. As a result, CP-based compounds had tensile and Charpy impact strength properties comparable to commercial reference, but lower modulus. CP-compounds showed glass transition temperature (Tg) over 58% and heat distortion temperature (HDT) 12% higher compared to reference. CAP with C16 had HDT 82.1 °C. All the compounds were 3D printable using granular printing, but CAP compounds had challenges with printed layer adhesion. This study shows the potential to tailor thermoplastic cellulose-based composite materials, although more research is needed before obtaining all-cellulose 3D printable composite material with high-performance.
Alkylative Aziridine Ring-Opening Reactions
Authors: Choi Jieun , Yu Taehwan , Ha Hyun-Joon
Published: March 2021

In this study, the highly strained three-membered aziridine ring was successfully activated as the aziridinium ion by alkylation of the ring nitrogen with a methyl, ethyl or allyl group, which was followed by ring opening with external nucleophiles such as acetate and azide. Such alkylative aziridine ring opening provides an easy route for the synthesis of various N-alkylated amine-containing molecules with concomitant introduction of an external nucleophile at either its α- or β-position.
Effect of Broccoli Residue and Wheat Straw Addition on Nitrous Oxide Emissions in Silt Loam Soil
Authors: Budhathoki Rajan , Panday Dinesh , Seiz Perik , Ruser Reiner , Müller Torsten
Published: March 2021

Nitrous oxide (N2O) is one of the main greenhouse gases and its emissions from vegetable production systems have brought a sustainability challenge. The objective of this study was to evaluate the potential of reducing N2O emissions from silt loam soil by mixing nitrogen (N)-rich broccoli (Brassica oleracea var. italica) residue with wheat straw or water-washed wheat straw. An experiment was conducted in randomized complete block design with five treatments; unamended or control (BS), wheat straw (+S), broccoli residue (+CR), broccoli residue and wheat straw (+CR+S) and broccoli residue and washed wheat straw (+CR+Sw) and was replicated four times. The +CR and +S were added at the rate of 3.5 kg and 2.0 kg fresh matter m−2 and their mixtures, +CR+S and +CR+Sw, were incorporated in 3.5 kg of silt loam soil at 60% water-filled pore space (WFPS) and packed in soil microcosms. Nitrous oxide emissions were measured once a day during the 14-day of study period. Daily fluxes of N2O were found to be reduced on +CR+W and +CR+Sw when compared to single-amended +CR treatment. Similarly, N2O fluxes on +CR+Sw (2772 µg N m−2 h−1) were significantly lower than +CR+S (3606 µg N m−2 h−1) soon after the amendment but did not vary significantly thereafter. Moreover, the amendment mixture, +CR+S and +Cr+Sw, resulted in lower net N2O emissions by 73.3% and 74.2%, respectively, relative to +CR treatment. While the results clearly suggest that the +CR+S or +CR+Sw reduced N2O emissions, it necessitated further studies, possibly by increasing the frequency of sampling to clarify if washed wheat straw would further mitigate N2O emissions from the vegetable production system.
Pd-Based Polysaccharide Hydrogels as Heterogeneous Catalysts for Oxidation of Aromatic Alcohols
Authors: Levy-Ontman Oshrat , Stamker Eliraz , Mor Vital , Wolfson Adi
Published: March 2021

Immobilization of Pd(OAc)2(TPPTS)2 in various renewable polysaccharides hydrogels, yielded heterogeneous catalysts that were successfully used, for the first time, in the aerobic oxidation of benzylic alcohol. The new catalysts were easily removed from the reaction mixture and recycled with some loss of activity. Among all tested polysaccharides, iota-carrageenan was found to be the most suitable support, using calcium chloride as a gelation agent.
Uptake and User Characteristics for Pharmacy-Based Contraception and Chlamydia Treatment: A Quantitative Retrospective Study from the UK
Authors: Gauly Julia , Atherton Helen , Ross Jonathan D. C.
Published: March 2021

The health provider Umbrella delivers several SRHS through more than 120 pharmacies in Birmingham (England). Umbrella pharmacy data collected between August 2015 and August 2018 were used to descriptively analyse the uptake and user characteristics for emergency contraception, short-acting oral contraception, condoms and chlamydia treatment. In total, 54,309 pharmacy visits were analysed. A total of 30,473 females presented for emergency contraception. Most were supplied with an emergency contraceptive pill (98.6%, 30,052 out of 30,473), which was levonorgestrel in 57.4% of cases (17,255 out of 30,052). Of those females who attended for short-acting oral contraception, 54.3% (1764 out of 3247) were provided with the progesterone-only pill. Of those who were given chlamydia treatment, the majority received doxycycline (76.8%, 454 out of 591). A total of 74% (14,888 out of 19,998) of those who requested condoms were not provided with specific instructions on their use. Pharmacies have the potential to make a substantial contribution to the delivery of an integrated sexual health service including rapid access to emergency contraception, convenient delivery of short-acting hormonal contraception and treatment of chlamydia. Appropriate education, support and audit is required to ensure the delivery of high-quality care.
Regularization of Autoencoders for Bank Client Profiling Based on Financial Transactions
Authors: Filchenkov Andrey , Khanzhina Natalia , Tsai Arina , Smetannikov Ivan
Published: March 2021

Predicting if a client is worth giving a loan—credit scoring—is one of the most essential and popular problems in banking. Predictive models for this goal are built on the assumption that there is a dependency between the client’s profile before the loan approval and their future behavior. However, circumstances that cause changes in the client’s behavior may not depend on their will and cannot be predicted by their profile. Such clients may be considered “noisy” as their eventual belonging to the defaulters class results rather from random factors than from some predictable rules. Excluding such clients from the dataset may be helpful in building more accurate predictive models. In this paper, we report on primary results on testing the hypothesis that a client can become a defaulter in two scenarios: intentionally and unintentionally. We verify our hypothesis applying data driven regularized classification using an autoencoder to client profiles. To model an intention as a hidden variable, we propose an especially designed regularizer for the autoencoder. The regularizer aims to obtain a representation of defaulters that includes a cluster of intentional defaulters and unintentional defaulters as outliers. The outliers were detected by our model and excluded from the dataset. This improved the credit scoring model and confirmed our hypothesis.
Motion Planning and Control of an Omnidirectional Mobile Robot in Dynamic Environments
Authors: Azizi Mahmood Reza , Rastegarpanah Alireza , Stolkin Rustam
Published: March 2021

Motion control in dynamic environments is one of the most important problems in using mobile robots in collaboration with humans and other robots. In this paper, the motion control of a four-Mecanum-wheeled omnidirectional mobile robot (OMR) in dynamic environments is studied. The robot’s differential equations of motion are extracted using Kane’s method and converted to discrete state space form. A nonlinear model predictive control (NMPC) strategy is designed based on the derived mathematical model to stabilize the robot in desired positions and orientations. As a main contribution of this work, the velocity obstacles (VO) approach is reformulated to be introduced in the NMPC system to avoid the robot from collision with moving and fixed obstacles online. Considering the robot’s physical restrictions, the parameters and functions used in the designed control system and collision avoidance strategy are determined through stability and performance analysis and some criteria are established for calculating the best values of these parameters. The effectiveness of the proposed controller and collision avoidance strategy is evaluated through a series of computer simulations. The simulation results show that the proposed strategy is efficient in stabilizing the robot in the desired configuration and in avoiding collision with obstacles, even in narrow spaces and with complicated arrangements of obstacles.
Sperm Global DNA Methylation (SGDM) in Semen of Healthy Dogs
Authors: Galdiero Giacomo , D’Anza Emanuele , de Angelis Cristina , Albarella Sara , Peretti Vincenzo , Pivonello Rosario , Ciotola Francesca
Published: March 2021

Male infertility is an emerging problem in both humans and animals, and the knowledge of its causes is the first step to identifying new diagnostic and therapeutic strategies. In humans, alteration of sperm DNA methylation have been related to poor quality semen, impaired seminal parameters, azoospermia and reduced fertility. Although semen analysis is routinely used to evaluate the male reproductive potential in the canine species, no authors have attempted to relate semen characteristics to the sperm global DNA methylation (SGDM). The aim of this study was to evaluate the SGDM level in healthy dogs and to correlate it with semen parameters that are currently used in dog semen analyses. Conventional and unconventional (sperm DNA fragmentation and SGDM) seminal parameters of thirty dogs from different breeds were evaluated. A positive correlation was found between SGDM and sperm concentration (r = 0.41; p < 0.05), and total sperm count (r = 0.61; p < 0.001); SGDM was significantly lower in oligozoospermic vs non-oligozoospermic dogs (4.3% vs. 8.7%; p < 0.005). Our findings suggest that SGDM levels are related to conventional seminal parameters, and could be used as a marker of testis function and spermatogenesis in dogs.
Трансформация бизнес-модели предприятия, внедряющего современные принципы построения производственной системы
Authors:   Рязанский государственный радиотехнический университет, Гудкова Оксана Евгеньевна
Published: March 2021

Сокращение государственного оборонного заказа потребовало от предприятий оборонно-промышленного комплекса срочного изменения структуры выпуска в пользу продукции гражданского назначения. Однако, их основная часть оказалась не готовой к решению этой задачи в силу консерватизма производственной системы, свойственной оборонным предприятиям. Как показало исследование, внедрение современных принципов организации производства требует изменения всей модели бизнеса, включая производственную, социальную подсистемы и подсистему управления предприятием. В статье приведены результаты преобразований бизнес-модели одного из оборонных предприятий, получившего за счет проведенных изменений существенный прирост выпуска гражданской продукции.