bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-cell analysis reveals that Jinwu Gutong capsule attenuates the inflammatory activity of synovial cells in osteoarthritis by inhibiting AKR1C3
Authors: Guo Junfeng , Tang Chuyue , Shu Zhao , Guo Junfeng , Tang Hong , Huang Pan , Ye Xiao , Liang Taotao , Tang Kanglai
Published: November 2022

Jinwu Gutong capsule (JGC) is a traditional Chinese medicine formula for the treatment of osteoarthritis (OA). Synovitis is a typical pathological change in OA and promotes disease progression. Elucidating the therapeutic mechanism of JGC is crucial for the precise treatment of OA synovitis. In this study, we demonstrate that JGC effectively inhibits hyperproliferation, attenuates inflammation, and promotes apoptosis of synovial cells. Through scRNA-seq data analysis of OA synovitis, we dissected two distinct cell fates that influence disease progression (one fate led to recovery while the other fate resulted in deterioration), which illustrates the principles of fate determination. By intersecting JGC targets with synovitis hub genes and then mimicking picomolar affinity interactions between bioactive compounds and binding pockets, we found that the quercetin-AKR1C3 pair exhibited the best affinity, indicating that this pair constitutes the most promising molecular mechanism. In vitro experiments confirmed that the expression of AKR1C3 in synovial cells was reduced after JGC addition. Further overexpression of AKR1C3 significantly attenuated the therapeutic efficacy of JGC. Thus, we revealed that JGC effectively treats OA synovitis by inhibiting AKR1C3 expression.
Variation of blubber thickness for three marine mammal species in the southern Baltic Sea
Authors: Siebert Ursula , Grilo Miguel L. , Kesselring Tina , Lehnert Kristina , Ronnenberg Katrin , Pawliczka Iwona , Galatius Anders , Kyhn Line A. , Dähne Michael , Gilles Anita
Published: November 2022

Evaluating populational trends of health condition has become an important topic for marine mammal populations under the Marine Strategy Framework Directive (MSFD). In the Baltic Sea, under the recommendation of Helsinki Commission (HELCOM), efforts have been undertaken to use blubber thickness as an indicator of energy reserves in marine mammals. Current values lack geographical representation from the entire Baltic Sea area and a large dataset is only available for grey seals (Halichoerus grypus) from Sweden and Finland. Knowledge on variation of blubber thickness related to geography throughout the Baltic Sea is important for its usage as an indicator. Such evaluation can provide important information about the energy reserves, and hence, food availability. It is expected that methodological standardization under HELCOM should include relevant datasets with good geographical coverage that can also account for natural variability in the resident marine mammal populations. In this study, seasonal and temporal trends of blubber thickness were evaluated for three marine mammal species—harbor seal (Phoca vitulina), grey seal (Halichoerus grypus) and harbor porpoise (Phocoena phocoena)—resident in the southern Baltic Sea collected and investigated under stranding networks. Additionally, the effects of age, season and sex were analyzed. Seasonal variation of blubber thickness was evident for all species, with harbor seals presenting more pronounced effects in adults and grey seals and harbor porpoises presenting more pronounced effects in juveniles. For harbor seals and porpoises, fluctuations were present over the years included in the analysis. In the seal species, blubber thickness values were generally higher in males. In harbor seals and porpoises, blubber thickness values differed between the age classes: while adult harbor seals displayed thicker blubber layers than juveniles, the opposite was observed for harbor porpoises. Furthermore, while an important initial screening tool, blubber thickness assessment cannot be considered a valid methodology for overall health assessment in marine mammals and should be complemented with data on specific health parameters developed for each species.
Pharmacological rescue of specific long QT variants of KCNQ1/KCNE1 channels
Authors: Zou Xinle , Wu Xiaoan , Sampson Kevin J. , Colecraft Henry M. , Larsson H. Peter , Kass Robert S.
Published: November 2022

The congenital Long QT Syndrome (LQTS) is an inherited disorder in which cardiac ventricular repolarization is delayed and predisposes patients to cardiac arrhythmias and sudden cardiac death. LQT1 and LQT5 are LQTS variants caused by mutations in KCNQ1 or KCNE1 genes respectively. KCNQ1 and KCNE1 co-assemble to form critical IKS potassium channels. Beta-blockers are the standard of care for the treatment of LQT1, however, doing so based on mechanisms other than correcting the loss-of-function of K+ channels. ML277 and R-L3 are compounds that enhance IKS channels and slow channel deactivation in a manner that is dependent on the stoichiometry of KCNE1 subunits in the assembled channels. In this paper, we used expression of IKS channels in Chinese hamster ovary (CHO) cells and Xenopus oocytes to study the potential of these two drugs (ML277 and R-L3) for the rescue of LQT1 and LQT5 mutant channels. We focused on the LQT1 mutation KCNQ1-S546L, and two LQT5 mutations, KCNE1-L51H and KCNE1-G52R. We found ML277 and R-L3 potentiated homozygote LQTS mutations in the IKS complexes-KCNE1-G52R and KCNE1-L51H and in heterogeneous IKS channel complexes which mimic heterogeneous expression of mutations in patients. ML277 and R-L3 increased the mutant IKS current amplitude and slowed current deactivation, but not in wild type (WT) IKS. We obtained similar results in the LQT1 mutant (KCNQ1 S546L/KCNE1) with ML277 and R-L3. ML277 and R-L3 had a similar effect on the LQT1 and LQT5 mutants, however, ML277 was more effective than R-L3 in this modulation. Importantly we found that not all LQT5 mutants expressed with KCNQ1 resulted in channels that are potentiated by these drugs as the KCNE1 mutant D76N inhibited drug action when expressed with KCNQ1. Thus, our work shows that by directly studying the treatment of LQT1 and LQT5 mutations with ML277 and R-L3, we will understand the potential utility of these activators as options in specific LQTS therapeutics.
ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2
Authors: Fan Kaijian , Fu Qinghui , Wei Qianhan , Jia Sinian , Zhao Anqi , Wang Tengteng , Cao Jie , Liu Yan , Ren Zhenjing , Liu Yunjun
Published: November 2022

Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown to involve in the splicing of different mitochondrial group II introns; however, the specific biological functions of maturases in maize are largely uncharacterized. In this study, we identified a maize ZmnMAT1 gene, which encodes a mitochondrion-localized type I maturase with an RT domain at N-terminus and an X domain at C-terminus. Loss-of-function mutation in ZmnMAT1 significantly reduced the splicing efficiencies of Nad1 intron 1 and Nad4 intron 2, and showed arrested embryogenesis and endosperm development, which may be related to impaired mitochondrial ultrastructure and function due to the destruction of the assembly and activity of complex I. Direct physical interaction was undetectable between ZmnMAT1 and the proteins associated with the splicing of Nad1 intron 1 and/or Nad4 intron 2 by yeast two-hybrid assays, suggesting the complexity of group II intron splicing in plants.
Silicon improves the photosynthetic performance of oat leaves infected with Puccinia graminis f. sp. avenae
Authors: Li Yinghao , Liu Jinghui , Lv Pin , Mi Junzhen , Zhao Baoping
Published: November 2022

Stem rust, caused by Puccinia graminis f. sp. avenae (Pga) is a key disease affecting oat production worldwide. Silicon (Si) plays an essential role in enhancing plant resistance against pathogens. However, the scientific evidence of Si-mediated stem rust resistance of oat from the photosynthetic perspective has not been reported. The specific objective of this research was to investigate the effects of Si application on disease inhibition, photosynthetic gas exchange parameters, light response parameters, photosynthetic pigments and chlorophyll fluorescence parameters under Pga infection. Our results illustrated that Si application significantly reduced rust severity while the other parameters like net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr) were significantly increased. Si application increased maximum photosynthetic rate (Pnmax) and light saturation point (LSP), while reduced the dark respiration rate (Rd) and light compensation point (LCP). The results also indicated that Si application significantly increased the activities of maximum fluorescence (Fm), variable fluorescence (Fv), maximum quantum yield of photosystem II (Fv/Fm), photochemical quenching (qP), photosynthetic performance index (PIABS), actual PSII quantum yield (ΦPSII), electron transfer rate (ETR), the absorbed light energy per unit reaction center (ABS/RC) and the dissipated energy per unit reaction center (DIo/RC), whereas it decreased the minimal fluorescence (Fo), non-photochemical quenching (NPQ), the absorbed light energy used for electron transfer per unit reaction center (ETo/RC) and the absorbed light energy used for reduction of QA per unit reaction center (TRo/RC). The contents of chlorophyll a, b and carotenoids were also increased due to the change in the activity of parameters due to Si application as mentioned above. In conclusion, the results of the current study suggests that Si imparts tolerance to the stem rust possibly by the underlying mechanisms of improving gas exchange performance, and efficiency of the photochemical compounds in oat leaves.
Co-elevation of atmospheric [CO2] and temperature alters photosynthetic capacity and instantaneous water use efficiency in rice cultivars in a cold-temperate region
Authors: Zhang Chunyu , Li Yansheng , Yu Zhenhua , Wang Guanghua , Liu Xiaobing , Liu Junjie , Liu Judong , Zhang Xingmei , Yin Kuide , Jin Jian
Published: November 2022

Crop photosynthetic capacity in response to climate change likely constrains crop productivity and adaptability to changing environments, which requests the investigation on the dynamics of photosynthetic parameters over growth season among varieties, especially in cold-temperate regions. Three Japonica rice cultivars i.e., Shoubaimao (SH), Hejiang 19 (HJ); Longjing 31, (LJ). were planted under the control, e[CO2] (700 μmol mol-1), warming (2°C above the air temperature) and the co-elevation of [CO2] and temperature in open-top chambers (OTC). The objective of this study is to examine the rice photosynthetic parameters, water use efficiency (WUE) and yield formation in responses to the co-elevation of [CO2] and temperature which is the main predicted features of future climate. e[CO2] significantly increased An of SH, HJ and LJ by 37%, 39% and 23% in comparison to 34%, 34% and 27% under elevated [CO2] plus warming, respectively. However, An had a weak response to warming for three cultivars. [CO2] and temperature co-elevation significantly decreased the stomatal conductance, resulting in a significant increase of the WUE. e[CO2] significantly increased Vc, max, Jmax and Jmax/Vc, max. e[CO2] significantly increased grain yield and grain number of all cultivars. The positive effect of co-elevation of [CO2] and temperature on grain yield was less than e[CO2]. Warming is likely to partially offset the increased photosynthetic rate caused by e[CO2]. The [CO2] and temperature co-elevation may be favorable to rice crop with increasing the photosynthetic ability of rice crop and improving water use efficiency. The present study provided evidence that the rice genotypic difference in photosynthetic potential under [CO2] and temperature co-elevation. Therefore, it is crucial to explore a broader range of phenotypes and cultivars to be applied to climate change response research, advancing the knowledge that climate change impacts rice crop under the cold-temperate climate region.
Yield and quality of alfalfa (Medicago sativa L.) in response to fertilizer application in China: A meta-analysis
Authors: Wan Weifan , Li Yuejin , Li Haigang
Published: November 2022

Introduction

In China, alfalfa (Medicago sativa L.) often grows in marginal land with poor soil fertility and suboptimal climate conditions. Alfalfa production cannot meet demands both in yield and quality. It is necessary to apply fertilizers to achieve high yields and produce high-quality alfalfa in China. However, there is no understanding on the impact of fertilizer application on alfalfa production and the possible optimal application rates across China.

Methods

We conducted a meta-analysis to explore the contribution of fertilizer application to the yield and quality of alfalfa based on a dataset from 86 studies published between 2004 and 2022.

Results and Discussion

The results showed that fertilizer application not only increased alfalfa yield by 19.2% but also improved alfalfa quality by increasing crude protein (CP) by 7.7% and decreasing acid detergent fibre by 2.9% and neutral detergent fibre by 1.8% overall compared to the non-fertilizer control levels. The combined nitrogen (N), phosphorus (P) and potassium (K) and combined NP fertilizer applications achieved the greatest yield and CP concentration increases of 27.0% and 13.5%, respectively. Considering both yield and quality, the optimal rate of fertilizer application ranged from 30 to 60 kg ha-1 for N, 120 to 150 kg ha-1 for P and less than 120 kg ha-1 for K. Meta-analysis further showed that the effect of fertilizer application on yield was greater in low soil organic matter (SOM) soils than in high SOM soils. In conclusion, fertilizer application is an effective strategy to improve the yield and quality of alfalfa in China, especially that grown in low SOM soils. This study is helpful for optimizing fertilization schedules of alfalfa in China.
Ascorbate synthesis as an alternative electron source for mitochondrial respiration: Possible implications for the plant performance
Authors: Matos Isabelle Faria , Morales Luis Miguel Mazorra , Santana Diederson Bortolini , Silva Gláucia Michelle Cosme , Gomes Mara Menezes de Assis , Ayub Ricardo Antônio , Costa José Hélio , Oliveira Jurandi Gonçalves de
Published: November 2022

The molecule vitamin C, in the chemical form of ascorbic acid (AsA), is known to be essential for the metabolism of humans and animals. Humans do not produce AsA, so they depend on plants as a source of vitamin C for their food. The AsA synthesis pathway occurs partially in the cytosol, but the last oxidation step is physically linked to the respiratory chain of plant mitochondria. This oxidation step is catalyzed byl-galactono-1,4-lactone dehydrogenase (l-GalLDH). This enzyme is not considered a limiting step for AsA production; however, it presents a distinguishing characteristic: thel-GalLDH can introduce electrons directly into the respiratory chain through cytochrome c (Cytc) and therefore can be considered an extramitochondrial electron source that bypasses the phosphorylating Complex III. The use of Cytc as electron acceptor has been debated in terms of its need for AsA synthesis, but little has been said in relation to its impact on the functioning of the respiratory chain. This work seeks to offer a new view about the possible changes that result of the link between AsA synthesis and the mitochondrial respiration. We hypothesized that some physiological alterations related to low AsA may be not only explained by the deficiency of this molecule but also by the changes in the respiratory function. We discussed some findings showing that respiratory mutants contained changes in AsA synthesis. Besides, recent works that also indicate that the excessive electron transportvial-GalLDH enzyme may affect other respiratory pathways. We proposed that Cytc reduction byl-GalLDH may be part of an alternative respiratory pathway that is active during AsA synthesis. Also, it is proposed that possible links of this pathway with other pathways of alternative electron transport in plant mitochondria may exist. The review suggests potential implications of this relationship, particularly for situations of stress. We hypothesized that this pathway of alternative electron input would serve as a strategy for adaptation of plant respiration to changing conditions.
(In)frequently asked questions: On types of frequency and their role(s) in heritage language variability
Authors: Perez-Cortes Silvia , Giancaspro David
Published: November 2022

In recent years, researchers have become increasingly interested in exploring frequency as a source of variability in heritage speakers’ (HSs) knowledge of their heritage language (HL). While many of these studies acknowledge that frequency can affect the shape of HL grammars, there is still no clear consensus about (a) what “frequency” means in the context of HL acquisition and (b) how to operationalize its multiple subtypes. In this paper, we provide a critical overview of frequency effects in HL research and their relevance for understanding patterns of inter/intra-speaker variability. To do so, we outline how prior research has defined, measured, and tested frequency, and present—as well as evaluate—novel methodological approaches and innovations recently implemented in the study of frequency effects, including a new analysis of how self-reported lexical frequency reliably predicts HSs’ production of subjunctive mood in Spanish. Our aim is to highlight the immense potential of such work for addressing long-standing questions about HL grammars and to propose new lines of inquiry that will open up additional pathways for understanding HL variability.
How exaptation mediates the effects of customer participation on low-cost innovation: The role of strategic flexibility
Authors: Tang Chaoyong , Shi Yongzhi , Zhu Xuechun , Li Yibo
Published: November 2022

With the advent of emerging markets, the need for low-cost innovation to meet the rising demands of people at the base of the pyramid has increased significantly. Although the critical influence of customer participation on new product development has been recognized, there have been few studies on the effects of customer participation on low-cost innovation. This study builds a moderated mediation model and explores the roles of customer participation on low-cost innovation. Based on the exaptation and strategic flexibility theories, the mediating role of exaptation and the moderating role of strategic flexibility are emphasized. A survey of 348 firms revealed that customer participation positively impacted both exaptation and low-cost innovation. In addition, exaptation mediated the correlation between customer participation and low-cost innovation. Resource flexibility negatively moderated the correlation between customer participation and exaptation and negatively moderated the mediating effect of exaptation. Furthermore, coordinate flexibility positively moderated the correlation between customer participation and exaptation and positively moderated the mediating effect of exaptation.
How the European Union legislations are tackling the burden of diabetes mellitus: A legal surveillance study
Authors: Mahrouseh Nour , Lovas Szabolcs , Njuguna Diana Wangeshi , Nellamkuzhi Noel Johny , Soares Andrade Carlos Alexandre , Sackey Wilhelmina Egyirba , Irawan Anggi Septia , Varga Orsolya
Published: November 2022

Introduction

Surveillance of the European Union's (EU) legislations on the prevention of diabetes mellitus (DM) is needed, to more effectively tackle the rising prevalence of DM.

Methods

This legal surveillance was carried out through a systematic search and screening, using EUR-Lex database to identify treaties, acts, and other legal documents for prevention of DM, non-communicable diseases (NCDs) and obesity, followed by their content analysis and assessment according to DM specific guidelines, target population and functional categories.

Results and discussion

We found 22 legislations aimed at preventing DM, NCDs and obesity, but only 5 out of them specifically addressed preventing DM. The aims of legislations covered a broad spectrum of themes indicated by DM specific guidelines, mostly initiatives of life-course approach in preventing DM, NCDs and obesity from the area of energy intake. The target group of most legislations was the general population; high-risk subgroups such as pregnant women were hardly ever the primary target group. Our results prove that the EU has made cross-sectoral legislative efforts to reduce the disease burden and prevent DM but does not exhaust all possibilities. However, given its persistently rising DM prevalence, it is imperative to make sure that DM is a top health priority for various EU authorities and is incorporated into new initiatives, policies and laws.
High chlamydia infection and its associated factors among patients seeking clinic-based STI services in Southern China: A preliminary cross-sectional study
Authors: Wang Honglin , Weng Rongxing , Zhang Chunlai , Ye Jianbin , Wen Lizhang , Li Jing , Lu Yongyi , Ning Ning , Hong Fuchang , Chen Xiangsheng , Cai Yumao
Published: November 2022

Objective

Chlamydia trachomatis (CT) infection is one of the most common sexually transmitted infections (STIs) worldwide. This study aimed to provide prevalence and associated factors data among patients seeking clinic-based STI services for estimating the disease burden of CT.

Study design and method

A cross-sectional survey was conducted among patients attending clinics for STI services. Patients' social-demographic and behavioral information was collected and CT infection was determined by nucleic acid amplification test (NAAT) with self-collected urine specimens. Associated factors were identified using logistic regression.

Results

Among the 8,324 participants, the overall prevalence was 9.0% with 10.7% for males and 8.3% for females respectively. Multivariate analysis showed that aged < 24 [adjusted odds ratio (aOR) = 1.27, 95% confidence interval (CI) = 1.01–1.59], being unmarried (aOR = 1.64, 95%CI = 1.35–2.00), having junior high school or below education level (aOR = 1.47, 95%CI = 1.13–1.91), having no access to health insurance (aOR = 1.27, 95%CI = 1.07–1.51), and being positive for Neisseria gonorrhoeae (NG, aOR = 4.49, 95%CI = 3.25–6.21) were significantly associated with CT infection.

Conclusion

We found that CT infection is prevalent among patients seeking clinic-based STI services in Southern China. Targeted interventions could be implemented for patients with a higher risk of CT infection including those aged < 24, being unmarried, having junior high school or below education level, having no access to health insurance, and being positive for NG. In addition, routine CT screening could be considered a public health strategy by the government.
Trends in global burden of diseases attributable to lead exposure in 204 countries and territories from 1990 to 2019
Authors: Zhou Nan , Huang Yue , Li Mingma , Zhou Lu , Jin Hui
Published: November 2022

Background

Lead hazards are ubiquitous in the environment, and lead exposure has been proved to damage human health. Nevertheless, there is limited data on the global burden of diseases attributable to lead exposure. In this study, we evaluated the temporal-spatial trend of disease burden caused by lead exposure in 204 countries and territories from 1990 to 2019.

Methods

Based on Global Burden of Disease (GBD) Study 2019, deaths, disability-adjusted life years (DALYs), age-standardized mortality rate (ASMR) and DALYs rate (ASDR) were estimated by region, country, sex and age. The estimated annual percentage change (EAPC) was calculated to assess the temporal trends of ASMR and ASDR between 1990 and 2019.

Results

Global deaths increased from 0.53 (95% UI: 0.31, 0.77) to 0.90 (95% UI: 0.55, 1.29) million, and the number of DALYs increased from 16.02 (95% UI: 10.32, 22.17) to 21.68 (95% UI: 13.81, 30.30) million between 1990 and 2019. China, India and Bangladesh were top three countries with the largest number of deaths and DALYs in 2019. The ASMR (per 100,000 population) decreased from 14.47 (95% UI: 8.40, 21.43) to 11.48 (95% UI: 7.00, 16.49) with EAPC of −0.75 (95% UI: −0.87, −0.64), and the ASDR (per 100,000 population) decreased from 378.01 (95% UI: 240.55, 524.18) to 267.52 (95% UI: 170.57, 373.44) with EAPC of −1.19 (95% UI: −1.32, −1.07). Most of disease burden of lead exposure occurred in the men and elderly population. Stroke and ischemic heart disease were two key sources of disease burden of lead exposure. Also, a negative association between sociodemographic index (SDI) and disease burden of lead exposure was observed.

Conclusions

Lead exposure poses a significant disease burden globally, and is still a great threat to public health. Primary prevention measures of reducing lead exposure in the environment are essential.
The application value of the Modified Early Warning Score combined with age and injury site scores in the evaluation of injuries in emergency trauma patients
Authors: Li Qing , Ren Yu-Qin , Qian Yu-Fei , Li Dan-Feng
Published: November 2022

Objective

To explore the application value of the Modified Early Warning Score (MEWS) combined with age and injury site scores in predicting the criticality of emergency trauma patients.

Methods

The traditional MEWS was modified by combining it with age and injury site scores to form a new MEWS combined scoring standard. The clinical data were collected from a total of 372 trauma patients from the emergency department of the Nantong First People's Hospital between June and December 2019. A retrospective analysis was conducted, and the patients were scored using the MEWS combined with age and injury site scores. The patients were grouped according to their prognoses and clinical outcomes. A statistical analysis was conducted based on the ranges of the combined scores, and the results of the combined scores of the different groups were compared.

Results

Among the 372 patients, the average score was 3.68 ± 1.25 points in the survival group, 8.33 ± 2.24 points in the death within 24 h group, and 8.38 ± 1.51 points in the death within 30 days of hospitalization group, and the differences were statistically significant (p < 0.05). The average score was 2.74 ± 0.69 points in the outpatient treatment group, 4.19 ± 0.72 points in the emergency stay group, 5.40 ± 0.70 points in the specialist inpatient group, 8.71 ± 2.31 points in the ICU group, and 7.82 ± 1.66 points in the specialist unplanned transfer to ICU group, with the differences between the groups being statistically significant (p < 0.05). The average length of hospital stay for patients with a joint score within the range of 6–8 points was 10.86 ± 2.47 days, with a direct ICU admission rate of 22.00% and an unplanned ICU admission rate of 16.00%. Patients with a joint score >8 points had an average length of hospital stay of 27.05 ± 4.85 days, with a direct ICU admission rate of 66.67% and an unplanned ICU admission rate of 33.33%.

Conclusion

Age and injury site are important high-risk indicators for trauma assessment, and using them in combination with the MEWS could improve the assessment of emergency patients with trauma, increasing the accuracy of pre-screening triage and reducing rescue time. Therefore, this joint scoring method might be worthy of clinical promotion and application.
Drivers of farmers' intentions to use eco-breeding: Integrating the theory of planned behavior and the norm activation model
Authors: Wang Xingdong , Lu Pan , Tang Jin , Gao Xueping , Liao Wenmei , Weng Zhenlin
Published: November 2022

Ecological breeding (eco-breeding) is a systematic ecological farming method for the benefit of the environment and human health, but farmers have adopted this method only to a lesser extent. The main objective of this study was to examine the factors that influence farmers' intentions to use eco-breeding methods. The study model was a combined application of the theory of planned behavior (TPB) and the normative activation model (NAM) to investigate the intentions of 527 Chinese farmers to engage in eco-breeding practices. Structural equation modeling analysis revealed that although the farmers' intention to adopt eco-breeding practices was influenced by both self-interested and altruistic motives, self-interested motives had a greater impact on the farmers' choices. Furthermore, favorable attitudes had the greatest effect on the farmers' intentions to implement eco-breeding, while past habits had no statistically significant effect on the intentions. Nonetheless, past habits significantly influenced attitudes and perceived behavior control in the combined model. In addition, the findings indicated that awareness of consequences had a significant effect on personal norms and the attribution of responsibility. Overall, the findings demonstrate the good efficiency as well as comprehensiveness of the integrated TPB-NAM in explaining the farmers' intentions to engage in eco-breeding practices. This study increases our understanding of the factors influencing the farmers' adoption of eco-breeding practices and helps to promote the adoption of eco-breeding in rural areas while providing a basis for the development of eco-breeding policy interventions.
What Is Fructose and How Does It Make Sweet Drinks Dangerous for Your Health?
Authors: Nieto-Mazzocco Elizabeth , Franco-Robles Elena , Jaramillo-Morales Osmar A. , Ozuna César
Published: November 2022

Nowadays, overweight and obesity are increasing in young people, resulting in several health problems. New scientific evidence shows that consuming too much fructose, a basic type of sugar, can cause some of these problems. Sugary foods such as soft drinks are sweetened with syrups that have very high fructose content. Drinking too much of these beverages results in weight gain from the accumulation of body fat. Scientists have also found that, in children, consuming excessive amounts of fructose can harm the liver, lungs, and heart. This article will explain how fructose in sweetened beverages leads to overweight and obesity in children and adolescents, highlighting the alarming number of young people all over the world who suffer from these diseases and what can be done to prevent this situation.
U-blade gamma 3 vs. gamma 3 nails for intertrochanteric hip fracture: Meta-analysis
Authors: Mariscal Gonzalo , Lorente Rafael , Barrios Carlos
Published: November 2022

Background and Objective

Intertrochanteric fracture is a growing problem in the traumatology department. The use of intramedullary devices has increased, representing the first treatment option in intertrochanteric fractures. U-Blade devices appeared to avoid rotation of the femoral head over the femoral neck. The aim of this study was to conduct a meta-analysis of the surgical treatment of intertrochanteric fractures comparing in terms of safety and efficacy the U-Blade Gamma 3 nail vs. the conventional Gamma 3 nail.

Methods

A literature search for intertrochanteric fracture 31A1–31A3 according to the AO foundation/orthopaedic trauma association (AO/OTA) classification was performed. Baseline characteristics of each article were obtained; radiological outcomes were tip apex distance (TAD), sliding distance (mm), cut-out rate, and lateralization rate. Surgery time (min) was also recorded. A meta-analysis was performed with ReviewManager 5.4.

Results

Five retrospective studies (n = 993 patients) were included. With respect to TAD and sliding distance, there were no differences between two groups [mean difference (MD) 0.47, 95% confidence interval (CI), −0.46 to 1.40] and (MD 0.39, 95% CI, 0.13–0.66). The cut-out rate and lateralization rate did not show differences between two groups (p > 0.05). Finally, surgery time was significantly higher in the U-Blade Gamma 3 group (MD −4.84, 95% CI, −7.22 to −2.46).

Conclusions

The use of U-Blade Gamma 3 did not show significant differences in the radiological results compared with the conventional Gamma 3 nail.
Endoscopic vs. microscopic stapes surgery: An anatomical feasibility study
Authors: Blijleven Esther E. , Willemsen Koen , Bleys Ronald L. A. W. , Stokroos Robert J. , Wegner Inge , Thomeer Henricus G. X. M.
Published: November 2022

Objectives

To investigate the feasibility of the endoscopic approach vs. microscopic approach during stapes surgery, focusing on the visualization of the important anatomical structures of the middle ear, the volume of the resected scutum and chorda tympani (CT) injury.

Methods

Fresh frozen human cadaveric heads underwent two stapes surgeries using an operating microscope on one ear and an endoscope on the other ear. The surgeon documented the visualization of critical landmarks, as well as exposure and injury of the CT. The volume of resected scutum was evaluated using cone beam computed tomography scanning and three-dimensional imaging.

Results

We performed endoscopic stapes surgery in 10 ears and microscopic stapes surgery in 11 ears. A stapes prosthesis was placed in all ears. The volume of bony scutum resection was significantly lower in the endoscopic group (median = 2.20 mm3, IQR = 4.17) than in the microscopic group (median 13.25 mm3, IQR = 8.71). No scutum was removed in two endoscopic ears, while scutum was removed in all microscopic ears. The endoscopic and microscopic group had similar CT injury.

Conclusions

This study showed that the endoscopic stapes surgery procedure is feasible and might be less invasive than microscopic stapes surgery. Future clinical prospective and functional studies will be needed to support our findings.
Maternal catalase supplementation regulates fatty acid metabolism and antioxidant ability of lactating sows and their offspring
Authors: Zhou Tiantian , Cheng Bei , Gao Lumin , Ren Fengyun , Guo Guanglun , Wassie Teketay , Wu Xin
Published: November 2022

Introduction and methods

As a crucial antioxidant enzyme, catalase (CAT) could destroy the cellular hydrogen peroxide to mitigate oxidative stress. The current study aimed to investigate the effects of maternal CAT supplementation from late gestation to day 14 of lactation on antioxidant ability and fatty acids metabolism with regard to the sow-piglet-axis. On day 95 of gestation, forty sows were divided into control (CON) group (fed a basal diet) and CAT group (fed a basal diet supplemented with 660 mg/kg CAT), the feeding experiment ended on day 14 of lactation.

Results

The lactating sows in the CAT group produced more milk, and had higher antioxidant enzymes activity including POD and GSH-Px (P < 0.05), lower content of serum LDL as well as plasmic C18:3n3 content (P < 0.05). Additionally, maternal CAT supplementation improved offspring's body weight at day 14 of nursing period and ADG (P < 0.05), and regulated the antioxidant ability as evidenced by decreased related enzymes activity such as T-AOC and CAT and changed genes expression level. It significantly affected lipid metabolism of suckling piglets manifested by increasing the serum ALT, CHOL, and LDL (P < 0.05) level and modulating plasma medium- and long-chain fatty acids (MCFAs and LCFAs), as well as regulating the genes expression involved in lipid metabolism.

Conclusion

Maternal CAT supplementation could regulate the fatty acid composition and enhance the antioxidant ability of sows and offspring during the lactating period and further promote the growth of suckling piglets. These findings might provide a reference value for the utilization of CAT as supplement for mother from late pregnancy to lactation period to promote the fatty acid metabolism of offspring.
Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
Authors: Han Mingzheng , Hu Wenfeng , Chen Tong , Guo Hanxing , Zhu Jianfeng , Chen Feng
Published: November 2022

Coccidiosis, an acute epidemic intestinal disease of poultry, is caused by the parasitic protozoan genus Eimeria, with Eimeria tenella being the most pathogenic spp. Novel approaches are required to address the limitations of current treatments for this disease. We investigated the effects of eight plant extracts and essential oils and their mixture on Eimeria tenella as potential treatments for coccidial infection. The anticoccidial effects of non-toxic concentrations of Punica granatum L. (0.005 mg/mL), Plantago asiatica L. (0.780 mg/mL), Bidens pilosa L. (0.390 mg/mL), Acalypha australis L. (0.390 mg/mL), Pteris multifida Poir (0.050 mg/mL), and Portulaca oleracea L. sp. Pl. (0.050 mg/mL) extracts; Artemisia argyi Levl. et Vant. (0.010 μL/mL) and Camellia sinensis (L.) O. Ktze (0.050 μL/mL) essential oils; and their mixture (0.500 mL/mL) on Eimeria tenella were determined using cell viability assays, flow cytometry, and in vivo studies. The eight plant extracts and essential oils and their mixture inhibited Eimeria tenella sporozoites from invading chicken embryo fibroblast cells in vitro. The extract and essential oil mixture improved the feed conversion ratio and body weight gain, reduced fecal oocyst excretion, substantially reduced the mortality of Eimeria tenella-infected chickens, and reduced Eimeria tenella-induced cecal damage in vivo. The results suggest that the extract and essential oil mixtures inhibit Eimeria tenella invasion both in vitro and in vivo, demonstrating their potential as anticoccidial agents.
The Association of Occupational Stress with Anxiety among Chinese Civil Pilots: The Moderating Role of Type A Behavior Pattern
Authors: Wang Yanlong , Guo Wei , Cheng Lin , Ji Ruipeng , Zhou Yizhi , Liu Xinhua , Zhao Yanzeng , Liu Ziyu , Wang Lijing
Published: November 2022

Pilots are highly susceptible to anxiety symptoms due to the uncertainty of the dangers of aviation operations and the potential terror and insecurity that pilots experience over time. This research aims to understand the complex relationship between the occupational stress and group anxiety symptoms of Chinese civil aviation pilots, and to analyze the type A behavior pattern (TABP) as a mediator for both issues. The occupational stress, anxiety symptoms and TABP of Chinese civil aviation pilots are investigated by using questionnaires based on the effort-reward-imbalance (ERI) scale, the Zung Self-Rating Anxiety Scale (SAS) and a TABP scale established based on Chinese people’s inherent characteristics. The study revealed that higher occupational stress experienced by Chinese civil aviation pilots exacerbated their anxiety symptoms, and that overcommitment and TABP mediated the relationship between occupational stress and anxiety symptoms.
Fecal Microbiota Transplantation Could Improve Chronic Diarrhea in Cynomolgus Monkey by Alleviating Inflammation and Modulating Gut Microbiota
Authors: Tian Puyuan , Gao Jiangmei , Liang Lifeng , Cui Bota , Hu Qiming , Zhou Wenfeng , Li Bihai , Liu Yiyan , Chen Tingtao , Rao Junhua , Wei Hong
Published: November 2022

Chronic diarrhea is associated with enteric dysbiosis and provokes the overuse of antibiotics. Fecal microbiota transplantation (FMT) is a promising therapy, but it shows discrepant clinical efficacy. Bacterial colonization in recipients has been studied, although little is known about the role of gut fungi and Archaea after FMT. In this study, we evaluated the efficacy of human-derived FMT on spontaneous chronic diarrhea cynomolgus monkeys and revealed the effector mechanisms. We demonstrated that FMT can mitigate the appearance of diarrheal symptoms and inhibit the increase in interleukin-6, interleukin-8, interleukin-1β, and interferon-γ and the decrease in interleukin-10 in serum. We confirmed that FMT restored the disturbance of gut bacteria by reducing the relative abundances of potential pathogens, including Cloacibacillus porcorum, Desulfovibrio desulfuricans, Erysipelotrichaceae bacterium 5_2_54FAA, and Erysipelotrichaceae bacterium 21_3, and increasing the levels of Lactobacillus fermentum and Lactobacillus ruminis CAG_367 in diarrheal monkeys. The metabolic pathways of healthy and FMT monkeys’ gut bacteria were enriched in amino acid metabolism, carbohydrate metabolism, and lipid metabolism, while the metabolic pathways of pre-FMT monkeys’ gut bacteria were enriched in antibiotic production. Moreover, a higher Ascomycota/Basidiomycota ratio, higher Aspergillus levels, and lower Trichosporon asahii abundance were present in intestinal fungi after FMT. Although the abundance of the Archaea Methanosphaera stastmanae did not change significantly, it was inversely correlated with the anti-inflammatory factor IL-4 after FMT. These results support the further development and application of FMT for chronic diarrhea.
Repetitive Anodal TDCS to the Frontal Cortex Increases the P300 during Working Memory Processing
Authors: Voegtle Angela , Reichert Christoph , Hinrichs Hermann , Sweeney-Reed Catherine M.
Published: November 2022

Transcranial direct current stimulation (TDCS) is a technique with which neuronal activity, and therefore potentially behavior, is modulated by applying weak electrical currents to the scalp. Application of TDCS to enhance working memory (WM) has shown promising but also contradictory results, and little emphasis has been placed on repeated stimulation protocols, in which effects are expected to be increased. We aimed to characterize potential behavioral and electrophysiological changes induced by TDCS during WM training and evaluate whether repetitive anodal TDCS has a greater modulatory impact on the processes underpinning WM than single-session stimulation. We examined the effects of single-session and repetitive anodal TDCS to the dorsolateral prefrontal cortex (DLPFC), targeting the frontal-parietal network, during a WM task in 20 healthy participants. TDCS had no significant impact on behavioral measures, including reaction time and accuracy. Analyzing the electrophysiological response, the P300 amplitude significantly increased following repetitive anodal TDCS, however, positively correlating with task performance. P300 changes were identified over the parietal cortex, which is known to engage with the frontal cortex during WM processing. These findings support the hypothesis that repetitive anodal TDCS modulates electrophysiological processes underlying WM.
An Experimental Study of Subliminal Self-Face Processing in Depersonalization–Derealization Disorder
Authors: Liu Shanshan , Jia Yuan , Zheng Sisi , Feng Sitong , Zhu Hong , Wang Rui , Jia Hongxiao
Published: November 2022

The self-perception or self-experience of patients with depersonalization/derealization disorder (DPD) is altered, leading to a profound disruption in self-awareness. The main aim of the study is to explore the characteristics of subliminal self-face processing in DPD patients. To our knowledge, this is the first experimental study that has measured and evaluated subliminal self-processing in DPD. To better understand this, we examined the ability of patients with DPD and healthy controls (HC) to identify pictures of faces using an experimental paradigm of breaking continuous flash suppression. There were 23 DPD outpatients from Beijing Anding Hospital, Capital Medical University and 23 matched HC who participated in this experiment. The time needed for a face to break into awareness was taken as the measure of participants’ subliminal processing of that face. The results indicated that there were significant differences between the DPD patients and HC in subliminal reaction times to different faces. Under experimental conditions, the average reaction response of self-face recognition in the HC group was significantly faster than for a famous face. However, this difference was not observed in DPD patients, which means that DPD patients did not show the processing advantage of their own faces as did the HC. The results suggest a deficit in subliminal self-face processing in DPD.
Preoperative Cancer Antigen 125 Level as Predictor for Complete Cytoreduction in Ovarian Cancer: A Prospective Cohort Study and Systematic Review
Authors: Brons Puck E. , Nieuwenhuyzen-de Boer Gatske M. , Ramakers Christian , Willemsen Sten , Kengsakul Malika , van Beekhuizen Heleen J.
Published: November 2022

Background: The tumor marker ‘cancer antigen 125’ (CA-125) plays a role in the management of women with advanced stage ovarian cancer. This study aims to describe the predictive value of pre-treatment CA-125 level and the reduction after neoadjuvant chemotherapy (NACT) on surgical outcome. Methods: A systematic review and a prospective clinical study were performed. Multiple databases were searched from database inception to April 2022. The clinical study is part of a randomized controlled trial named “PlaComOv-study”. A regression analysis was performed to demonstrate correlations between preoperative CA-125 levels, CA-125 reduction after NACT, and surgical outcome. Results: Fourteen relevant articles were analyzed of which eleven reported that lower preoperative CA-125 levels were associated with a higher probability of complete cytoreduction. In the clinical study, 326 patients with FIGO stage IIIB-IV ovarian cancer who underwent CRS were enrolled from 2018 to 2020. Patients who underwent interval CRS with preoperative CA-125 levels ≤35 kU/L had higher odds of achieving complete CRS than patients with CA-125 level >35 kU/L (85% vs. 67%, OR 2.79, 95%CI 1.44–5.41, p = 0.002). In multivariable analysis with presence of ascites and peritoneal carcinomatosis, normalized preoperative CA-125 did not appear as a significant predictor for complete CRS. Conclusions: In literature, preoperative CA-125 levels ≤35 kU/L were associated with a significant higher percentage of complete CRS in univariable analysis. According to our cohort study, preoperative CA-125 level ≤35 kU/L cannot independently predict surgical outcome either for primary or interval CRS.
Tracers in Gastric Cancer Surgery
Authors: Li Zhiyan , Li Xianghui , Zhu Xudong , Ai Shichao , Guan Wenxian , Liu Song
Published: November 2022

The treatment of gastric cancer mainly depends on radical gastrectomy. Determination of appropriate surgical margins and adequate lymph node (LN) resection are two major surgical steps that directly correlate with prognosis in gastric cancer. Due to the expanding use of minimally invasive procedures, it is no longer possible to locate tumors and LNs through touch. As an alternative, tracers have begun to enter the field due to their capacities for intraoperative visualization. Herein, we summarize the application of contemporary tracers in gastric cancer surgery, including isosulfan blue, methylene blue, patent blue, indocyanine green, carbon particles, and radioactive tracers. Their mechanisms, administration methods, detection efficiency, and challenges, as well as perspectives on them, are also outlined.