bluuebunny/crossref_metadata_2025 huggingface dataset:
The Relative Effects of Economic Growth, Environmental Pollution and Non-Communicable Diseases on Health Expenditures in European Union Countries
Authors: Badulescu Daniel , Simut Ramona , Badulescu Alina , Badulescu Andrei-Vlad
Published: December 2019

National and global health policies are increasingly recognizing the key role of the environment in human health development, which is related to its economic and social determinants, such as income level, technical progress, education, quality of jobs, inequality, education or lifestyle. Research has shown that the increase of GDP (Gross Domestic Product) per capita can provide additional funds for health but also for environmental protection. However, often, economic growth is associated with the accelerated degradation of the environment, and this in turn will result in an exponential increase in harmful emissions and will implicitly determine the increasing occurrence of non-communicable diseases (NCDs), mainly cardiovascular diseases, cancers and respiratory diseases. In this paper, we investigate the role and effects of economic growth, environmental pollution and non-communicable diseases on health expenditures, for the case of EU (European Union) countries during 2000–2014. In order to investigate the long-term and the short-term relationship between them, we have employed the Panel Autoregressive Distributed Lag (ARDL) method. Using the Pedroni-Johansen cointegration methods, we found that the variables are cointegrated. The findings of this study show that economic growth is one of the most important factors influencing the health expenditures both in the long- and short-run in all the 28 EU countries. With regards to the influence of CO2 emissions on health expenditure, we have found a negative impact in the short-run and a positive impact on the long-run. We have also introduced an interaction between NCDs and environmental expenditure as independent variable, a product variable. Finally, we have found that in all the three estimated models, the variation in environmental expenditure produces changes in NCDs’ effect on health expenditure.
Cadmium and Lead Decrease Cell–Cell Aggregation and Increase Migration and Invasion in Renca Mouse Renal Cell Carcinoma Cells
Authors: Akin Ryan , Hannibal David , Loida Margaret , Stevens Emily M. , Grunz-Borgmann Elizabeth A. , Parrish Alan R.
Published: December 2019

Metastatic renal cell carcinoma (RCC) remains an important clinical issue; the 5-year survival rate of patients with metastasis is approximately 12%, while it is 93% in those with localized disease. There is evidence that blood cadmium and lead levels are elevated in RCC. The current studies were designed to assess the impact of cadmium and lead on the progression of RCC. The disruption of homotypic cell–cell adhesion is an essential step in epithelial-to-mesenchymal transition and tumor metastasis. Therefore, we examined the impact of cadmium and lead on the cadherin/catenin complex in Renca cells—a mouse RCC cell line. Lead, but not cadmium, induced a concentration-dependent loss of E-cadherin, while cadmium, but not lead, increased p120-catenin expression, specifically isoform 1 expression. Lead also induced a substantial increase in matrix metalloproteinase-9 levels. Both cadmium and lead significantly decreased the number of Renca cell aggregates, consistent with the disruption of the cadherin/catenin complex. Both metals enhanced wound healing in a scratch assay, and increased cell migration and invasion. These data suggest that cadmium and lead promote RCC progression.
The Putatively Specific Synthetic REV-ERB Agonist SR9009 Inhibits IgE- and IL-33-Mediated Mast Cell Activation Independently of the Circadian Clock
Authors: Ishimaru Kayoko , Nakajima Shotaro , Yu Guannan , Nakamura Yuki , Nakao Atsuhito
Published: December 2019

The cell-autonomous circadian clock regulates IgE- and IL-33-mediated mast cell activation, both of which are key events in the development of allergic diseases. Accordingly, clock modifiers could be used to treat allergic diseases, as well as many other circadian-related diseases, such as sleep and metabolic disorders. The nuclear receptors REV-ERB-α and -β (REV-ERBs) are crucial components of the circadian clockwork. Efforts to pharmacologically target REV-ERBs using putatively specific synthetic agonists, particularly SR9009, have yielded beneficial effects on sleep and metabolism. Here, we sought to determine whether REV-ERBs are functional in the circadian clockwork in mast cells and, if so, whether SR9009 affects IgE- and IL-33-mediated mast cell activation. Bone marrow-derived mast cells (BMMCs) obtained from wild-type mice expressed REV-ERBs, and SR9009 or other synthetic REV-ERBs agonists affected the mast cell clockwork. SR9009 inhibited IgE- and IL-33-mediated mast cell activation in wild-type BMMCs in association with inhibition of Gab2/PI3K and NF-κB activation. Unexpectedly, these suppressive effects of SR9009 were observed in BMMCs following mutation of the core circadian gene Clock. These findings suggest that SR9009 inhibits IgE- and IL-33-mediated mast cell activation independently of the functional circadian clock activity. Thus, SR9009 or other synthetic REV-ERB agonists may have potential for anti-allergic agents.
Glucose Tolerance-Improving Activity of Helichrysoside in Mice and Its Structural Requirements for Promoting Glucose and Lipid Metabolism
Authors: Morikawa Toshio , Nagatomo Akifumi , Oka Takahiro , Miki Yoshinobu , Taira Norihisa , Shibano-Kitahara Megumi , Hori Yuichiro , Muraoka Osamu , Ninomiya Kiyofumi
Published: December 2019

An acylated flavonol glycoside, helichrysoside, at a dose of 10 mg/kg/day per os for 14 days, improved the glucose tolerance in mice without affecting the food intake, visceral fat weight, liver weight, and other plasma parameters. In this study, using hepatoblastoma-derived HepG2 cells, helichrysoside, trans-tiliroside, and kaempferol 3-O-β-D-glucopyranoside enhanced glucose consumption from the medium, but their aglycones and p-coumaric acid did not show this activity. In addition, several acylated flavonol glycosides were synthesized to clarify the structural requirements for lipid metabolism using HepG2 cells. The results showed that helichrysoside and related analogs significantly inhibited triglyceride (TG) accumulation in these cells. The inhibition by helichrysoside was more potent than that by other acylated flavonol glycosides, related flavonol glycosides, and organic acids. As for the TG metabolism-promoting activity in high glucose-pretreated HepG2 cells, helichrysoside, related analogs, and their aglycones were found to significantly reduce the TG contents in HepG2 cells. However, the desacyl flavonol glycosides and organic acids derived from the acyl groups did not exhibit an inhibitory impact on the TG contents in HepG2 cells. These results suggest that the existence of the acyl moiety at the 6'' position in the D-glucopyranosyl part is essential for glucose and lipid metabolism-promoting activities.
Mechanisms Underlying Bone Loss Associated with Gut Inflammation
Authors: Ke Ke , Arra Manoj , Abu-Amer Yousef
Published: December 2019

Patients with gastrointestinal diseases frequently suffer from skeletal abnormality, characterized by reduced bone mineral density, increased fracture risk, and/or joint inflammation. This pathological process is characterized by altered immune cell activity and elevated inflammatory cytokines in the bone marrow microenvironment due to disrupted gut immune response. Gastrointestinal disease is recognized as an immune malfunction driven by multiple factors, including cytokines and signaling molecules. However, the mechanism by which intestinal inflammation magnified by gut-residing actors stimulates bone loss remains to be elucidated. In this article, we discuss the main risk factors potentially contributing to intestinal disease-associated bone loss, and summarize current animal models, illustrating gut-bone axis to bridge the gap between intestinal inflammation and skeletal disease.
Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion
Authors: Sedova Ksenia A. , Bernikova Olesya G. , Cuprova Julia I. , Ivanova Alexandra D. , Kutaeva Galina A. , Pliss Michael G. , Lopatina Ekaterina V. , Vaykshnorayte Marina A. , Diez Emiliano R. , Azarov Jan E.
Published: December 2019

Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and “ischemic” conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.
The mTOR Signaling Pathway Activity and Vitamin D Availability Control the Expression of Most Autism Predisposition Genes
Authors: Trifonova Ekaterina A. , Klimenko Alexandra I. , Mustafin Zakhar S. , Lashin Sergey A. , Kochetov Alex V.
Published: December 2019

Autism spectrum disorder (ASD) has a strong and complex genetic component with an estimate of more than 1000 genes implicated cataloged in SFARI (Simon′s Foundation Autism Research Initiative) gene database. A significant part of both syndromic and idiopathic autism cases can be attributed to disorders caused by the mechanistic target of rapamycin (mTOR)-dependent translation deregulation. We conducted gene-set analyses and revealed that 606 out of 1053 genes (58%) included in the SFARI Gene database and 179 out of 281 genes (64%) included in the first three categories of the database (“high confidence”, “strong candidate”, and “suggestive evidence”) could be attributed to one of the four groups: 1. FMRP (fragile X mental retardation protein) target genes, 2. mTOR signaling network genes, 3. mTOR-modulated genes, 4. vitamin D3 sensitive genes. The additional gene network analysis revealed 43 new genes and 127 new interactions, so in the whole 222 out of 281 (79%) high scored genes from SFARI Gene database were connected with mTOR signaling activity and/or dependent on vitamin D3 availability directly or indirectly. We hypothesized that genetic and/or environment mTOR hyperactivation, including provocation by vitamin D deficiency, might be a common mechanism controlling the expressivity of most autism predisposition genes and even core symptoms of autism.
An LSTM Model for Predicting Cross-Platform Bursts of Social Media Activity
Authors: Hajiakhoond Bidoki Neda , Mantzaris Alexander V. , Sukthankar Gita
Published: December 2019

Burst analysis and prediction is a fundamental problem in social network analysis, since user activities have been shown to have an intrinsically bursty nature. Bursts may also be a signal of topics that are of growing real-world interest. Since bursts can be caused by exogenous phenomena and are indicative of burgeoning popularity, leveraging cross platform social media data may be valuable for predicting bursts within a single social media platform. A Long-Short-Term-Memory (LSTM) model is proposed in order to capture the temporal dependencies and associations based upon activity information. The data used to test the model was collected from Twitter, Github, and Reddit. Our results show that the LSTM based model is able to leverage the complex cross-platform dynamics to predict bursts. In situations where information gathering from platforms of concern is not possible the learned model can provide a prediction for whether bursts on another platform can be expected.
Prospects for Genetic Improvement in Objective Measurements of Body Colour in Pacific Whiteleg Shrimp (Litopenaeus vannamei)
Authors: Giang Cao Truong , Knibb Wayne , Muu Tran The , Ninh Nguyen Huu , Nguyen Nguyen Hong
Published: December 2019

Body colour, together with growth and survival, are traits of commercial importance in Pacific whiteleg shrimp (Litopenaeus vannamei). However, heritability estimates for objective measurements of body colour are not available in Whiteleg shrimp species, including L. vannamei. Further, the effect of genotype by environment interactions (G × E) on this trait (i.e., the objective measures of body colour) and its genetic associations with growth are not known in this species. The present study presented the first attempt at understanding the genetic architecture of this complex character (body colour) that is of economic significance to the shrimp aquaculture sector world-wide. Specifically, we investigated the quantitative genetic basis of shrimp colour, while using the measurement tool (colorimeter) for a Whiteleg shrimp population reared in two contrasting environments. A total of 5464 shrimp had the objective measurements of body colour (lightness, yellowness, and redness) and growth trait records (weight, length and width). They were the offspring of 204 dams and 197 sires. The restricted maximum likelihood mixed model analysis showed that there were heritable additive genetic components for all of the measurements of shrimp colour, with the heritability (h2) ranging from 0.11–0.55. The h2 estimates for redness and yellowness traits differed between the two environments (h2 = 0.66–0.82 in Khanhhoa vs. 0.00–0.03 in Haiphong). However, the heritability for colour traits was moderate (0.11–0.55) when the two environments were combined. There is existence of (co)-genetic variances between the studied traits. The genetic correlations of body traits with redness or yellowness colour of the shrimp were moderate and positive (a*: 0.13–0.32 for redness and b*: 0.19–0.40 for yellowness). The effect of G × E interactions on shrimp colours could be important, as the genetic correlations for these traits between the two environments were low (−0.41 to 0.16). Our results showed that the genetic improvement for body colour can be achieved through direct selection and the increased redness colour is also expected to have favorable impacts on growth traits. Breeding programs to improve shrimp colour should account for the effects of environmental factors.
Synergistic Use of Synthetic Aperture Radar and Optical Imagery to Monitor Surface Accumulation of Cyanobacteria in the Curonian Lagoon
Authors: De Santi Francesca , Luciani Giulia , Bresciani Mariano , Giardino Claudia , Lovergine Francesco Paolo , Pasquariello Guido , Vaiciute Diana , De Carolis Giacomo
Published: December 2019

Phytoplankton blooms in internal water bodies are an unpleasant sight that often emerges on top like a layer of foam containing high concentrations of toxins (scum event). Monitoring the concentration of algae and the occurrence of scum in lakes and lagoons has become a topic of interest for management and science. Optical remote sensing is a validated tool but unfortunately it is highly hindered by clouds. For regions with frequent cloud cover, such as the Baltic region, this means loss of data, which limits the purpose of sensing to spatially and temporally characterize any scum for a comprehensive ecological analysis. The aim of this paper is to investigate whether the use of synthetic aperture radar (SAR) images can compensate for the weaknesses of optical images for cyanobacteria bloom monitoring purposes in the event of cloudy skies. A “ready to use” approach to detect cyanobacteria bloom in the Curonian Lagoon based on the level 2 ocean product of Sentinel-1 images is proposed. This method is empirically validated for the images of summer/autumn 2018 of the Curonian Lagoon.
Characterization of Carotenoid Biosynthesis in Newly Isolated Deinococcus sp. AJ005 and Investigation of the Effects of Environmental Conditions on Cell Growth and Carotenoid Biosynthesis
Authors: Choi Jun Young , Lee Kunjoong , Lee Pyung Cheon
Published: December 2019

Our purpose was to characterize the structures of deinoxanthin from Deinococcus sp. AJ005. The latter is a novel reddish strain and was found to synthesize two main acyclic carotenoids: deinoxanthin and its derivative. The derivative (2-keto-deinoxanthin) contains a 2-keto functional group instead of a 2-hydroxyl group on a β-ionone ring. A deinoxanthin biosynthesis pathway of Deinococcus sp. AJ005 involving eight putative enzymes was proposed according to genome annotation analysis and chemical identification of deinoxanthin. Optimal culture pH and temperature for Deinococcus sp. AJ005 growth were pH 7.4 and 20 °C. Sucrose as a carbon source significantly enhanced the cell growth in comparison with glucose, glycerol, maltose, lactose, and galactose. When batch fermentation was performed in a bioreactor containing 40g/L sucrose, total carotenoid production was 650% higher than that in a medium without sucrose supplementation. The culture conditions found in this study should provide the basis for the development of fermentation strategies for the production of deinoxanthin and of its derivative by means of Deinococcus sp. AJ005.
Bayesian Space–Time Analysis of Brain Cancer Incidence in Southern Ontario, Canada: 2010–2013
Authors: Persad Ravi Ancil
Published: December 2019

Canada has one of the highest incidence rates of brain cancer in the world. This study investigates the space–time variation of brain cancer risk across Southern Ontario, Canada. A Bayesian spatio-temporal regression model is used to estimate the relative risk of brain cancer in the 12 spatial health units of Southern Ontario over a four-year period (2010–2013). This work also explores the association between brain cancer and two potential risk factors: traumatic head injury (THI) and excess body fat (EBF). Across all areal units from 2010–2013, results show that the relative risk of brain cancer ranged from 0.83 (95% credible interval (CI) 0.74–0.91) to 1.26 (95% CI 1.13–1.41). Over the years, the eastern and western health units had persistently higher risk levels compared to those in the central areas. Results suggest that areas with elevated THI rates and EBF levels were also potentially associated with higher brain cancer relative risk. Findings revealed that the mean temporal trend for cancer risk progression in the region smoothly decreased over time. Overall, 50% of the health units displayed area-specific trends which were higher than the region’s average, thus indicating a slower decrease in cancer rates for these areas in comparison to the mean trend.
Characterization of Mechanisms Lowering Susceptibility to Flumequine among Bacteria Isolated from Chilean Salmonid Farms
Authors: Concha Christopher , Miranda Claudio D. , Hurtado Luz , Romero Jaime
Published: December 2019

Despite their great importance for human therapy, quinolones are still used in Chilean salmon farming, with flumequine and oxolinic acid currently approved for use in this industry. The aim of this study was to improve our knowledge of the mechanisms conferring low susceptibility or resistance to quinolones among bacteria recovered from Chilean salmon farms. Sixty-five isolates exhibiting resistance, reduced susceptibility, or susceptibility to flumequine recovered from salmon farms were identified by their 16S rRNA genes, detecting a high predominance of species belonging to the Pseudomonas genus (52%). The minimum inhibitory concentrations (MIC) of flumequine in the absence and presence of the efflux pump inhibitor (EPI) Phe-Arg-β-naphthylamide and resistance patterns of isolates were determined by a microdilution broth and disk diffusion assays, respectively, observing MIC values ranging from 0.25 to >64 µg/mL and a high level of multi-resistance (96%), mostly showing resistance to florfenicol and oxytetracycline. Furthermore, mechanisms conferring low susceptibility to quinolones mediated by efflux pump activity, quinolone target mutations, or horizontally acquired resistance genes (qepA, oqxA, aac(6′)-lb-cr, qnr) were investigated. Among isolates exhibiting resistance to flumequine (≥16 µg/mL), the occurrence of chromosomal mutations in target protein GyrA appears to be unusual (three out of 15), contrasting with the high incidence of mutations in GyrB (14 out of 17). Bacterial isolates showing resistance or reduced susceptibility to quinolones mediated by efflux pumps appear to be highly prevalent (49 isolates, 75%), thus suggesting a major role of intrinsic resistance mediated by active efflux.
Cold Adapted Nitrosospira sp.: A Potential Crucial Contributor of Ammonia Oxidation in Cryosols of Permafrost-Affected Landscapes in Northeast Siberia
Authors: Sanders Tina , Fiencke Claudia , Hüpeden Jennifer , Pfeiffer Eva Maria , Spieck Eva
Published: December 2019

Permafrost-affected landscape soils are rich in organic matter and contain a high fraction of organic nitrogen, but much of this organic matter remains inaccessible due to nitrogen limitation. Microbial nitrification is a key process in the nitrogen cycle, controlling the availability of dissolved inorganic nitrogen (DIN) such as ammonium and nitrate. In this study, we investigate the microbial diversity of canonical nitrifiers and their potential nitrifying activity in the active layer of different Arctic cryosols in the Lena River Delta in North-East Siberia. These cryosols are located on Samoylov Island, which has two geomorphological landscapes with mineral soils in the modern floodplain and organic-rich soils in the low-centered polygonal tundra of the Holocene river terrace. Microcosm incubations show that the highest potential ammonia oxidation rates are found in low organic soils, and the rates depend on organic matter content and quality, vegetation cover, and water content. As shown by 16S rRNA amplicon sequencing, nitrifiers represented 0.6% to 6.2% of the total microbial community. More than 50% of the nitrifiers belonged to the genus Nitrosospira. Based on PCR amoA analysis, ammonia-oxidizing bacteria (AOB) were found in nearly all soil types, whereas ammonia-oxidizing archaea (AOA) were only detected in low-organic soils. In cultivation-based approaches, mainly Nitrosospira-like AOB were enriched and characterized as psychrotolerant, with temperature optima slightly above 20 °C. This study suggests a ubiquitous distribution of ammonia-oxidizing microorganisms (bacteria and archaea) in permafrost-affected landscapes of Siberia with cold-adapted AOB, especially of the genus Nitrosospira, as potentially crucial ammonia oxidizers in the cryosols.
Efficacy of Kombucha Obtained from Green, Oolong, and Black Teas on Inhibition of Pathogenic Bacteria, Antioxidation, and Toxicity on Colorectal Cancer Cell Line
Authors: Kaewkod Thida , Bovonsombut Sakunnee , Tragoolpua Yingmanee
Published: December 2019

Kombucha tea is a refreshing beverage that is produced from the fermentation of tea leaves. In this study, kombucha tea was prepared using 1% green tea, oolong tea, and black tea, and 10% sucrose with acetic acid bacteria and yeast. The pH values of the kombucha tea were found to be in a range of 2.70–2.94 at 15 days of fermentation. The lowest pH value of 2.70 was recorded in the kombucha prepared from black tea. The total acidity of kombucha prepared from black tea was the highest by 16.75 g/L and it was still maintained after heat treatment by boiling and after autoclaved. Six organic acids: glucuronic, gluconic, D-saccharic acid 1,4-lactone, ascorbic, acetic, and succinic acid in kombucha tea were detected by HPLC with the optimization for organic acids detection using isocratic elution buffer with C18 conventional column. The highest level of organic acid was gluconic acid. Kombucha prepared from green tea revealed the highest phenolic content and antioxidation against DPPH radicals by 1.248 and 2.642 mg gallic acid/mL kombucha, respectively. Moreover, pathogenic enteric bacteria: Escherichia coli. E. coli O157:H7. Shigella dysenteriae, Salmonella Typhi, and Vibrio cholera were inhibited by kombucha and heat-denatured kombucha with diameter of the inhibition zones ranged from 15.0 ± 0.0–25.0 ± 0.0 mm. In addition, kombucha prepared from green tea and black tea demonstrated toxicity on Caco-2 colorectal cancer cells. Therefore, kombucha tea could be considered as a potential source of the antioxidation, inhibition of pathogenic enteric bacteria, and toxicity on colorectal cancer cells.
Study of the Inhibitory Effects of Enteral Nutrition Formula on Indomethacin-Induced Gastric Lesions in Mice
Authors: Yoshimi Takeshi , Yamagishi Yoshiaki , Kanegawa Issei , Suda Megumi , Saiki Rei , Tanaka Ken-ichiro , Goda Hitomi , Kudo Toshiyuki , Ito Kiyomi
Published: December 2019

We investigated the effects of enteral nutrition formula on non-steroidal anti-inflammatory drug (NSAID)-induced gastric lesions in mice. Male ICR mice aged 7–9 weeks old were fasted, then orally given either purified water, Mermed® One, or 2-fold diluted Terumeal® 2.0α as enteral nutrition (25 or 50 mL/kg each). Indomethacin (IND) was orally administered at 20 mg/kg after 30 min, and the stomach was removed 6 h later and fixed in formalin. The number and area of lesions in the stomachs of the mice given enteral nutrition showed a significant, dose-dependent decrease compared to the purified water-treated group, and no significant difference was seen between the two enteral nutrition-treated groups. Comparable time courses of plasma IND concentrations suggest that enteral nutrition does not inhibit gastrointestinal absorption of IND. Our findings indicate that administering enteral nutrition could inhibit the onset of NSAID-induced gastric ulcers.
Dynamical Freeze-Out Phenomena: The Case of K±, φ Transverse Momentum Spectra in Collisions of Au(1.23 A GeV) + Au
Authors: Rabe Benjamin , Kämpfer Burkhard
Published: December 2019

We argue for a continuous (dynamical) kinetic freeze-out of K ± , ϕ observed at midrapidity in collisions of Au(1.23 A GeV) + Au. The simulations, by means of a transport model of Boltzmann-Ühling-Uhlenbeck (BUU) type, point to time independent transverse momentum slope parameters after 20 fm/c. The complex interplay of expansion dynamics and strangeness production/exchange/absorption as well as elastic scatterings involved in the reaction network does not support the previous interpretation of a late freeze-out of K − due to larger cross sections.
High Molecular Weight Chitosan-Complexed RNA Nanoadjuvant for Effective Cancer Immunotherapy
Authors: Choi Jin Joo , Le Quoc-Viet , Kim Dongho , Kim Young Bong , Shim Gayong , Oh Yu-Kyoung
Published: December 2019

Nucleic acid-based adjuvants have recently emerged as promising candidates for use in cancer vaccines to induce tumor-suppressing immune cells. In this study, we tested whether complexation of a nucleic acid-based adjuvant with chitosan (CTS) modulates immune adjuvant functions. As a nucleic acid-based adjuvant, we used toll-like receptor 3-recognizing RNA adjuvant (RA). Negatively charged RA formed nanoscale polyplexes with cationic CTS that possessed positive zeta potentials. RA/CTS polyplexes exerted dendritic cell (DC)-maturation effects without causing significant DC toxicity. This DC-maturation effect was CTS molecular weight dependent, with RA/CTS polyplexes with a CTS molecular weight of 340 kDa (RA/CTS 340K) producing the greatest effect. Subcutaneous injection of RA/CTS 340K polyplexes with the model tumor antigen ovalbumin exerted a preventive effect against challenge by ovalbumin-expressing tumor cells. It also provided greater inhibitory effects against a second challenge with the same tumor cells compared with other treatments. These protective effects of subcutaneous RA/CTS polyplex treatment were associated with the highest tumor antigen-specific humoral and cellular immune responses after tumor challenge, and with the greatest infiltration of CD4 helper T cell and CD8 T cell into the tumor tissues. Mice vaccinated with ovalbumin and RA/CTS polyplexes showed complete survival, even after repeated challenge with tumor cells. Our results suggest the potential of RA/CTS polyplexes as effective nanoadjuvants in the design of tumor vaccines and cancer immunotherapy.
Repurposing Dihydropyridines for Treatment of Helicobacter pylori Infection
Authors: González Andrés , Casado Javier , Chueca Eduardo , Salillas Sandra , Velázquez-Campoy Adrián , Espinosa Angarica Vladimir , Bénejat Lucie , Guignard Jérome , Giese Alban , Sancho Javier , Lehours Philippe , Lanas Ángel
Published: December 2019

Antibiotic resistance is a major cause of the increasing failures in the current eradication therapies against Helicobacter pylori. In this scenario, repurposing drugs could be a valuable strategy to fast-track novel antimicrobial agents. In the present study, we analyzed the inhibitory capability of 1,4-dihydropyridine (DHP) antihypertensive drugs on the essential function of the H. pylori response regulator HsrA and investigated both the in vitro antimicrobial activities and the in vivo efficacy of DHP treatments against H. pylori. Six different commercially available and highly prescribed DHP drugs—namely, Nifedipine, Nicardipine, Nisoldipine, Nimodipine, Nitrendipine, and Lercanidipine—noticeably inhibited the DNA binding activity of HsrA and exhibited potent bactericidal activities against both metronidazole- and clarithromycin-resistant strains of H. pylori, with minimal inhibitory concentration (MIC) values in the range of 4 to 32 mg/L. The dynamics of the decline in the bacterial counts at 2 × MIC appeared to be correlated with the lipophilicity of the drugs, suggesting different translocation efficiencies of DHPs across the bacterial membrane. Oral treatments with 100 mg/kg/day of marketed formulations of Nimodipine or Nitrendipine in combination with omeprazole significantly reduced the H. pylori gastric colonization in mice. The results presented here support a novel therapeutic solution for treatment of antibiotic-resistant H. pylori infections.
Combined Modality Therapy Based on Hybrid Gold Nanostars Coated with Temperature Sensitive Liposomes to Overcome Paclitaxel-Resistance in Hepatic Carcinoma
Authors: Zhu Hongyan , Han Weili , Gan Ye , Li Qiaofeng , Li Xiaolan , Shao Lanlan , Zhu Dan , Guo Hongwei
Published: December 2019

In this study, we prepared gold nanostar (GNS) composite nanoparticles containing siRNA of cyclooxygenase-2(siCOX-2) that were modified by tumor targeting ligand 2-deoxyglucose (DG) and transmembrane peptide 9-poly-D-arginine (9R) to form siCOX-2(9R/DG-GNS). Paclitaxel loaded temperature sensitive liposomes (PTX-TSL) were surface-modified to produce PTX-TSL-siCOX-2(9R/DG-GNS) displaying homogeneous star-shaped structures of suitable size (293.93 nm ± 3.21) and zeta potentials (2.47 mV ± 0.22). PTX-TSL-siCOX-2(9R/DG-GNS) had a high thermal conversion efficiency under 808 nm laser radiation and a superior transfection efficiency, which may be related to the targeting effects of DG and increased heat induced membrane permeability. COX-2 expression in HepG2/PTX cells was significantly suppressed by PTX-TSL-siCOX-2(9R/DG-GNS) in high temperatures. The co-delivery system inhibited drug-resistant cell growth rates by ≥77% and increased the cell apoptosis rate about 47% at elevated temperatures. PTX-TSL and siCOX-2 loaded gold nanostar particles, therefore, show promise for overcoming tumor resistance.
Aptamers as Delivery Agents of siRNA and Chimeric Formulations for the Treatment of Cancer
Authors: Dinis Ano Bom Ana Paula , da Costa Neves Patrícia Cristina , Bonacossa de Almeida Carlos Eduardo , Silva Dilson , Missailidis Sotiris
Published: December 2019

Both aptamers and siRNA technologies have now reached maturity, and both have been validated with a product in the market. However, although pegaptanib reached the market some time ago, there has been a slow process for new aptamers to follow. Today, some 40 aptamers are in the market, but many in combination with siRNAs, in the form of specific delivery agents. This combination offers the potential to explore the high affinity and specificity of aptamers, the silencing power of siRNA, and, at times, the cytotoxicity of chemotherapy molecules in powerful combinations that promise to delivery new and potent therapies. In this review, we report new developments in the field, following up from our previous work, more specifically on the use of aptamers as delivery agents of siRNA in nanoparticle formulations, alone or in combination with chemotherapy, for the treatment of cancer.
Evolution of Interfacial Friction Angle and Contact Area of Polymer Pellets during the Initial Stage of Ultrasonic Plasticization
Authors: Jiang Bingyan , Zou Yang , Wei Guomeng , Wu Wangqing
Published: December 2019

Interfacial friction heating is one of the leading heat generation mechanisms during the initial stage of ultrasonic plasticization of polymer pellets, which has a significant influence on the subsequent viscoelastic heating according to our previous study. The interfacial friction angle and contact area of polymer pellets are critical boundary conditions for the analysis of interfacial frictional heating of polymer pellets. However, the duration of the interfacial friction heating is extremely short in ultrasonic plasticization, and the polymer pellets are randomly distributed in the cylindrical barrel, resulting in the characterization of the distribution of the interfacial friction angle and contact area to be a challenge. In this work, the interfacial friction angle of the polymer pellets in the partially plasticized samples of polymethyl methacrylate (PMMA), polypropylene (PP), and nylon66 (PA66) were characterized by a super-high magnification lens zoom 3D microscope. The influence of trigger pressure, plasticizing pressure, ultrasonic amplitude, and vibration time on the interfacial friction angle and the contact area of the polymer pellets were studied by a single factor experiment. The results show that the compaction degree of the plasticized samples could be enhanced by increasing the level of the process parameters. With the increasing parameter level, the proportion of interfacial friction angle in the range of 0–10° and 80–90° increased, while the proportion in the range of 30–60° decreased accordingly. The proportion of the contact area of the polymer pellets was increased up to 50% of the interfacial friction area which includes the upper, lower, and side area of the cylindrical plasticized sample.
Experimental and Numerical Investigation of Dustfall Effect on Remote Sensing Retrieval Accuracy of Chlorophyll Content
Authors: Ma Baodong , Li Xuexin , Liang Aiman , Chen Yuteng , Che Defu
Published: December 2019

Chlorophyll is the dominant pigment in the photosynthetic light-harvesting complexes that is related to the physiological function of leaves and is responsible for light absorption and energy transfer. Dust pollution has become an environmental problem in many areas in China, indicating that accurately estimating chlorophyll content of vegetation using remote sensing for assessing the vegetation growth status in dusty areas is vital. However, dust deposited on the leaf may affect the chlorophyll content retrieval accuracy. Thus, quantitatively studying the dustfall effect is essential. Using selected vegetation indices (VIs), the medium resolution imaging spectrometer terrestrial chlorophyll index (MTCI), and the double difference index (DD), we studied the retrieval accuracy of chlorophyll content at the leaf scale under dusty environments based on a laboratory experiment and spectra simulation. First, the retrieval accuracy under different dustfall amounts was studied based on a laboratory experiment. Then, the relationship between dustfall amount and fractional dustfall cover (FDC) was experimentally analyzed for spectra simulation of dusty leaves. Based on spectral data simulated using a PROSPECT-based mixture model, the sensitivity of VIs to dust under different chlorophyll contents was analyzed comprehensively, and the MTCI was modified to reduce its sensitivity to dust. The results showed that (1) according to experimental investigation, the DD model provides low retrieval accuracy, the MTCI model is highly accurate when the dustfall amount is less than 80 g/m2, and the retrieval accuracy decreases significantly when the dustfall amount is more than 80 g/m2; (2) a logarithmic relationship exists between FDC and dustfall amount, and the PROSPECT-based mixture model can simulate the leaf spectra under different dustfall amounts and different chlorophyll contents with a root mean square error of 0.015; and (3) according to numerical investigation, MTCI’s sensitivity to dust in the chlorophyll content range of 25 to 60 μg/cm2 is lower than in other chlorophyll content ranges; DD’s sensitivity to dust was generally high throughout the whole chlorophyll content range. These findings may contribute to quantitatively understanding the dustfall effect on the retrieval of chlorophyll content and would help to accurately retrieve chlorophyll content in dusty areas using remote sensing.
Monitoring the Risk of the Electric Component Imposed on a Pilot During Light Aircraft Operations in a High-Frequency Electromagnetic Field
Authors: Michałowska Joanna , Tofil Arkadiusz , Józwik Jerzy , Pytka Jarosław , Legutko Stanisław , Siemiątkowski Zbigniew , Łukaszewicz Andrzej
Published: December 2019

High-frequency electromagnetic fields can have a negative effect on both the human body and electronic devices. The devices and systems utilized in radio communications constitute the most numerous sources of electromagnetic fields. The following research investigates values of the electric component of electromagnetic field intensification determined with the ESM 140 dosimeter during the flights of four aircrafts—Cessna C152, Cessna C172, Aero AT3 R100, and Robinson R44 Raven helicopter—from the airport in Depultycze Krolewskie near Chelm, Poland. The point of reference for the obtained results were the normative limits of the electromagnetic field that can affect a pilot in the course of a flight. The maximum value registered by the dosimeter was E = 3.307 V/m for GSM 1800 frequencies.
LDAKM-EIoT: Lightweight Device Authentication and Key Management Mechanism for Edge-Based IoT Deployment
Authors: Wazid Mohammad , Das Ashok Kumar , Shetty Sachin , J. P. C. Rodrigues Joel , Park Youngho
Published: December 2019

In recent years, edge computing has emerged as a new concept in the computing paradigm that empowers several future technologies, such as 5G, vehicle-to-vehicle communications, and the Internet of Things (IoT), by providing cloud computing facilities, as well as services to the end users. However, open communication among the entities in an edge based IoT environment makes it vulnerable to various potential attacks that are executed by an adversary. Device authentication is one of the prominent techniques in security that permits an IoT device to authenticate mutually with a cloud server with the help of an edge node. If authentication is successful, they establish a session key between them for secure communication. To achieve this goal, a novel device authentication and key management mechanism for the edge based IoT environment, called the lightweight authentication and key management scheme for the edge based IoT environment (LDAKM-EIoT), was designed. The detailed security analysis and formal security verification conducted by the widely used “Automated Validation of Internet Security Protocols and Applications (AVISPA)” tool prove that the proposed LDAKM-EIoT is secure against several attack vectors that exist in the infrastructure of the edge based IoT environment. The elaborated comparative analysis of the proposed LDAKM-EIoT and different closely related schemes provides evidence that LDAKM-EIoT is more secure with less communication and computation costs. Finally, the network performance parameters are calculated and analyzed using the NS2 simulation to demonstrate the practical facets of the proposed LDAKM-EIoT.
IoTNet: An Efficient and Accurate Convolutional Neural Network for IoT Devices
Authors: Lawrence Tom , Zhang Li
Published: December 2019

Two main approaches exist when deploying a Convolutional Neural Network (CNN) on resource-constrained IoT devices: either scale a large model down or use a small model designed specifically for resource-constrained environments. Small architectures typically trade accuracy for computational cost by performing convolutions as depth-wise convolutions rather than standard convolutions like in large networks. Large models focus primarily on state-of-the-art performance and often struggle to scale down sufficiently. We propose a new model, namely IoTNet, designed for resource-constrained environments which achieves state-of-the-art performance within the domain of small efficient models. IoTNet trades accuracy with computational cost differently from existing methods by factorizing standard 3 × 3 convolutions into pairs of 1 × 3 and 3 × 1 standard convolutions, rather than performing depth-wise convolutions. We benchmark IoTNet against state-of-the-art efficiency-focused models and scaled-down large architectures on data sets which best match the complexity of problems faced in resource-constrained environments. We compare model accuracy and the number of floating-point operations (FLOPs) performed as a measure of efficiency. We report state-of-the-art accuracy improvement over MobileNetV2 on CIFAR-10 of 13.43% with 39% fewer FLOPs, over ShuffleNet on Street View House Numbers (SVHN) of 6.49% with 31.8% fewer FLOPs and over MobileNet on German Traffic Sign Recognition Benchmark (GTSRB) of 5% with 0.38% fewer FLOPs.
Non-Invasive Continuous Blood-Pressure Monitoring Models Based on Photoplethysmography and Electrocardiography
Authors: Wu Haiyan , Ji Zhong , Li Mengze
Published: December 2019

Blood pressure is an extremely important blood hemodynamic parameter. The pulse wave contains abundant blood-pressure information, and the convenience and non-invasivity of its measurement make it ideal for non-invasive continuous monitoring of blood pressure. Based on combined photoplethysmography and electrocardiogram signals, this study aimed to extract the waveform information, introduce individual characteristics, and construct systolic and diastolic blood-pressure (SBP and DBP) estimation models using the back-propagation error (BP) neural network. During the model construction process, the mean impact value method was employed to investigate the impact of each feature on the model output and reduce feature redundancy. Moreover, the multiple population genetic algorithm was applied to optimize the BP neural network and determine the initial weights and threshold of the network. Finally, the models were integrated for further optimization to generate the final individualized continuous blood-pressure monitoring models. The results showed that the predicted values of the model in this study correlated significantly with the measured values of the electronic sphygmomanometer. The estimation errors of the model met the Association for the Advancement of Medical Instrumentation (AAMI) criteria (the SBP error was 2.5909 ± 3.4148 mmHg, and the DBP error was 2.6890 ± 3.3117 mmHg) and the Grade A British Hypertension Society criteria.
Three-Dimensional Empirical AoA Localization Technique for Indoor Applications
Authors: Alma’aitah Abdallah , Alsaify Baha’ , Bani-Hani Raed
Published: December 2019

Small and pervasive devices have been increasingly used to identify and track objects automatically. Consequently, several low-cost localization schemes have been proposed in the literature based on angle of arrival (AoA), time difference of arrival (TDoA), received signal strength indicator (RSSI) or their combinations. In this paper, we propose a three-dimensional empirical AoA localization (TDEAL) technique for battery-powered devices. The proposed technique processes the AoA measurements at fixed reader nodes to estimate the locations of the tags. The proposed technique provides localization accuracy that mitigates non-linear empirical errors in AoA measurements. We utilize two omni-directional antenna arrays at each fixed reader node to estimate the location vector. With multiple location estimations from different fixed reader nodes, each estimated location is assigned a weight that is inversely proportional to the AoA phase-difference error. Furthermore, the actual AoA parabolic formula of the location is approximated to a cone to simplify the location calculation process. The proposed localization technique has a low hardware cost, low computational requirements, and precise location estimates. Based on the performance evaluation, significant location accuracy is achieved by TDEAL; where, for instance, an average error margin of less than 13 cm is achieved using 10 readers in an area of   10   m ×   10   m . TDEAL can be utilized to provide reference points when integrated with a relative (e.g., inertial navigation systems) localization systems.
Surface Temperature Simulation of Lunar Dayside and Its Geological Applications: A Case in Sinus Iridum
Authors: Zhang Jidong , Ping Jinsong , Zeng Zhaofa , Yang Yongzhang , Li Xiangyue , Wang Mingyuan
Published: December 2019

Lunar surface temperature is one of the fundamental thermophysical parameters of the lunar regolith, which is of great significance to the interpretation of remote-sensing thermal data. In this study, a daytime surface temperature model is established focusing on the lunar superficial layer with high spatial-temporal resolution. The physical parameters at the time of interest are adopted, including effective solar irradiance, lunar libration, large-scale topographic shading, and surrounding diffuse reflection. Thereafter, the 1/64° temperature distributions at five local times are quantitatively generated and analyzed in Sinus Iridum. Also, combined with Chang’E-2 microwave radiometer (CELMS) data and Diviner thermal infrared (TIR) data, the spectral emissivity distributions are estimated as a potential geological application of the simulated surface temperature. The results are as follows: (1) daytime surface temperature in Sinus Iridum is significantly affected by the local topography and observation time, and the influence of diffuse reflection energy is obvious; (2) the emissivity distributions provide a new way to understand the thermophysical properties difference of lunar regolith at different depths; (3) the influence of lunar orbiting revolution and precession on surface temperature should be analyzed carefully, which shows the importance of using the parameters at the time of interest.
The Problematics of Assessing Trans Identity in Survey Research: A Modest Proposal for Improving Question Design
Authors: Ryan J. Michael
Published: December 2019

One of the central issues facing the trans community today is not only to be counted, but also how to be properly counted. If and how trans people are counted has a huge impact on what we know, or what we think we know, about the trans community. When trans people are not counted, we know nothing, but when trans people are counted incorrectly, the results can be even worse. The question addressed in this paper, therefore, is how to develop a question(s) that will more accurately account for the trans population on national surveys. By drawing on cognitive interviews testing a gender identity and sexual identity question for a national official health survey, an argument is made for an improved method of understanding trans measurement on surveys.