bluuebunny/crossref_metadata_2025 huggingface dataset:
Selective Elucidation of Living Microbial Communities in Fermented Grains of Chinese Baijiu: Development of a Technique Integrating Propidium Monoazide Probe Pretreatment and Amplicon Sequencing
Authors: Bo Tao , Zhang Jiaojiao , Zong Enxiang , Lv Na , Bai Baoqing , Yang Yukun , Zhang Jinhua , Fan Sanhong
Published: June 2024

The fermentation process of Chinese Baijiu’s fermented grains involves the intricate succession and metabolism of microbial communities, collectively shaping the Baijiu’s quality. Understanding the composition and succession of these living microbial communities within fermented grains is crucial for comprehending fermentation and flavor formation mechanisms. However, conducting high-throughput analysis of living microbial communities within the complex microbial system of fermented grains poses significant challenges. Thus, this study addressed this challenge by devising a high-throughput analysis framework using light-flavor Baijiu as a model. This framework combined propidium monoazide (PMA) pretreatment technology with amplicon sequencing techniques. Optimal PMA treatment parameters, including a concentration of 50 μM and incubation in darkness for 5 min followed by an exposure incubation period of 5 min, were identified. Utilizing this protocol, viable microorganism biomass ranging from 8.71 × 106 to 1.47 × 108 copies/μL was successfully detected in fermented grain samples. Subsequent amplicon sequencing analysis revealed distinct microbial community structures between untreated and PMA-treated groups, with notable differences in relative abundance compositions, particularly in dominant species such as Lactobacillus, Bacillus, Pediococcus, Saccharomycopsis, Issatchenkia and Pichia, as identified by LEfSe analysis. The results of this study confirmed the efficacy of PMA-amplicon sequencing technology for analyzing living microbial communities in fermented grains and furnished a methodological framework for investigating living microbial communities in diverse traditional fermented foods. This technical framework holds considerable significance for advancing our understanding of the fermentation mechanisms intrinsic to traditional fermented foods.
An Integrated Pipeline and Overexpression of a Novel Efflux Transporter, YoeA, Significantly Increases Plipastatin Production in Bacillus subtilis
Authors: Wang Mengxi , Zheng Jie , Sun Sen , Wu Zichao , Shao Yuting , Xiang Jiahui , Yin Chenyue , Sedjoah Rita Cindy Aye Ayire , Xin Zhihong
Published: June 2024

Plipastatin, an antimicrobial peptide produced by Bacillus subtilis, exhibits remarkable antimicrobial activity against a diverse range of pathogenic bacteria and fungi. However, the practical application of plipastatin has been significantly hampered by its low yield in wild Bacillus species. Here, the native promoters of both the plipastatin operon and the sfp gene in the mono-producing strain M-24 were replaced by the constitutive promoter P43, resulting in plipastatin titers being increased by 27% (607 mg/mL) and 50% (717 mg/mL), respectively. Overexpression of long chain fatty acid coenzyme A ligase (LCFA) increased the yield of plipastatin by 105% (980 mg/mL). A new efflux transporter, YoeA, was identified as a MATE (multidrug and toxic compound extrusion) family member, overexpression of yoeA enhanced plipastatin production to 1233 mg/mL, an increase of 157%, and knockout of yoeA decreased plipastatin production by 70%; in contrast, overexpression or knockout of yoeA in mono-producing surfactin and iturin engineered strains only slightly affected their production, demonstrating that YoeA acts as the major exporter for plipastatin. Co-overexpression of lcfA and yoeA improved plipastatin production to 1890 mg/mL, which was further elevated to 2060 mg/mL after abrB gene deletion. Lastly, the use of optimized culture medium achieved 2514 mg/mL plipastatin production, which was 5.26-fold higher than that of the initial strain. These results suggest that multiple strain engineering is an effective strategy for increasing lipopeptide production, and identification of the novel transport efflux protein YoeA provides new insights into the regulation and industrial application of plipastatin.
Wave-Particle Interactions in Astrophysical Plasmas
Authors: Pérez-De-Tejada Héctor
Published: June 2024

Dissipation processes derived from the kinetic theory of gases (shear viscosity and heat conduction) are employed to examine the solar wind that interacts with planetary ionospheres. The purpose of this study is to estimate the mean free path of wave-particle interactions that produce a continuum response in the plasma behavior. Wave-particle interactions are necessary to support the fluid dynamic interpretation that accounts for the interpretation of various features measured in a solar wind–planet ionosphere region; namely, (i) the transport of solar wind momentum to an upper ionosphere in the presence of a velocity shear, and (ii) plasma heating produced by momentum transport. From measurements conducted in the solar wind interaction with the Venus ionosphere, it is possible to estimate that in general terms, the mean free path of wave-particle interactions reaches λH ≥ 1000 km values that are comparable to the gyration radius of the solar wind particles in their Larmor motion within the local solar wind magnetic field. Similar values are also applicable to conditions measured by the Mars ionosphere and in cometary plasma wakes. Considerations are made in regard to the stochastic trajectories of the plasma particles that have been implied from the measurements made in planetary environments. At the same time, it is as possible that the same phenomenon is applicable to the interaction of stellar winds with the ionosphere of exoplanets, and also in regions where streaming ionized gases reach objects that are subject to rotational motion in other astrophysical problems (galactic flow–plasma interactions, black holes, etc.).
Application of Machine Learning Models for Improving Discharge Prediction in Ungauged Watershed: A Case Study in East DuPage, Illinois
Authors: Asadollahi Amin , Magar Binod Ale , Poudel Bishal , Sohrabifar Asyeh , Kalra Ajay
Published: June 2024

Accurate flood prediction models and effective flood preparedness rely on thoroughly understanding rainfall–runoff dynamics. Similarly, effective rainfall–runoff models account for multiple interrelated parameters for robust runoff prediction. Process-based physical models offer valuable insights into hydrological processes, but their effectiveness can be hindered by data limitations or difficulties in acquiring specific data. Motivated by the frequent flooding events and limited data availability in the East Branch DuPage watershed, Illinois, this study addresses a critical gap in research by investigating effective discharge prediction methods. In this study, two significant machine learning (ML) models, artificial neural network (ANN) and support vector machine (SVM), were employed for discharge prediction. Historical data spanning from 2006 to 2021 were utilized to assess the performance of the models. Hyperparameter tuning was performed on the models to optimize their performance, and root mean square error (RMSE), Nash–Sutcliffe efficiency (NSE), percent bias (PBIAS), coefficient of determination (R2), and the normalized root mean squared error (NRMSE) were used as evaluation metrics. Although both machine learning models demonstrated strong performance, the analysis revealed that the ANN model emerged as the more reliable option for predicting discharge in the watershed. Crucially, the ANN model surpassed the SVM model’s performance, achieving superior accuracy in predicting peak discharge events within the study area. Our findings have the potential to assist decision-makers and communities in implementing more dependable flood mitigation strategies, particularly in regions where hydrology data are limited.
Glacies—A New Open Access Journal
Authors: Fassnacht Steven R.
Published: June 2024

Glacies means “the ice” in Latin [...]
Psychometric Properties of the Knowledge of Hydration among Foreign Students of Óbuda University, Hungary
Authors: Ogbolu Melvin Omone , Eniade Olanrewaju D. , Vincze Miklós , Kozlovszky Miklós
Published: June 2024

It is known that the quantity, makeup, and distribution of bodily fluids have a significant impact on the cognitive health, physiological health, and cell activity of human beings. This narrative could be influenced by the level of knowledge about hydration, dehydration, and the practice of Adequate Water Intake (AWI) of an individual based on the recommended daily Total Water Intake (TWI) by either the World Health Organization (WHO) or the European Food Safety Authority (EFSA). In this study, we have developed and validated a scale to adequately measure knowledge of the recommended daily Total Water Intake (TWI) practices among foreign students at Óbuda University, Hungary. Hence, we implemented an 11-item scale to measure the Knowledge of Hydration (KH-11) and evaluate its psychometric properties among students. This study is an online cross-sectional study assessing water intake knowledge with the use of the KH-11 tool among 323 students with ages ranging from 18 to 35 years, who have enrolled for at least two semesters at the University. The statistical analysis performed was reliability (using Cronbach alpha ≥ 70%) and factor analysis. Knowledge levels were categorized as poor (<50%), intermediate (50–70%), or adequate (71–100%). The intraclass correlation, chi-square, and rotated component matrix were also estimated and reported. Data were analyzed using SPSS version 25. Cronbach’s alpha analysis revealed that the KH-11 had an overall good reliability with a value of 0.80, where the survey items had an acceptable level of consistency ranging from 0.75 to 0.81 and demonstrated sufficient independence from each other as Pearson’s R within factors was positive and ranged from 0.02 to 0.74. In evaluating the participants’ knowledge of hydration, the total possible score for the scale is 72, while the mean score for the KH-11 was 55.2 ± 11.61 SD, and the factor analysis model yielded an acceptable fit (χ2 = 3259.4, p = 0.000). We recorded a high-level positive concordance of 0.770 with an average intraclass correlation of 0.80 at a 95% CI, where p-value = 0.000. Our findings show that the majority (66.3%) of the students have a good knowledge of hydration. However, the skewed distribution of the knowledge scores suggests that some may have lower levels of knowledge, which may warrant further study to improve knowledge in those students.
Secondary Prevention via Case Managers in Stroke Patients: A Cost-Effectiveness Analysis of Claims Data from German Statutory Health Insurance Providers
Authors: Duevel Juliane , Gruhn Sebastian , Grosser John , Elkenkamp Svenja , Greiner Wolfgang
Published: June 2024

Strokes remain a leading cause of death and disability worldwide. The STROKE OWL study evaluated a novel case management approach for patients with stroke (modified Rankin Scale 0–4) or transient ischemic attack (TIA) who received support across healthcare settings and secondary prevention training from case managers for one year. The primary aim of this quasi-experimental study was a reduction in stroke recurrence. Here, we report the results of a health economic analysis of the STROKE OWL study, conducted in accordance with CHEERS guidelines. The calculations were based on claims data of cooperating statutory health insurance companies. In addition to a regression analysis for cost comparison, the incremental cost-effectiveness ratio was determined, and a probabilistic sensitivity analysis was carried out. In total, 1167 patients per group were included in the analysis. The intervention group incurred 32.3% higher direct costs (p < 0.001) than the control group. With a difference of EUR 1384.78 (95% CI: [1.2384–1.4143], p < 0.0001) and a 5.32% increase in hazards for the intervention group (HR = 1.0532, 95% CI: [0.7869–1.4096], p = 0.7274) resulting in an ICER of EUR 260.30, we found that the case management intervention dominated in the total stroke population, even for an arbitrarily high willingness to pay. In the TIA subgroup, however, the intervention was cost-effective even for a low willingness to pay. Our results are limited by small samples for both TIA and severe stroke patients and by claims data heterogeneity for some cost components, which had to be excluded from the analysis. Future research should investigate the cost-effectiveness of case management interventions for both severe stroke and TIA populations using appropriate data.
Poultry-Based Amendments and Cover Crop Residues Enhance Nutrient Cycling and Soil Health in Greenhouse Conditions
Authors: Freidenreich Ariel , Pelegrina Gabriel , Victores Samantha , Maltais-Landry Gabriel
Published: June 2024

Organic producers have few certified organic options to meet crop nitrogen (N) demand. Poultry-based amendments, including manures and processed fertilizers from livestock waste (e.g., feather meal), are commonly used in these systems, but synchronizing nutrient release with plant demand is challenging. Cover crop residues are also used in organic systems and interact with amendments to affect soil health and nutrient cycling. We conducted a greenhouse study to quantify the effects of four cover crop residues (millet, sorghum sudangrass, cowpea, sunn hemp) and three amendments (heat-treated poultry manure, poultry manure biochar, organic fertilizer) on spinach. We measured spinach yield and nutrient uptake; soil inorganic N; total soil carbon (C) and N; and two soil health indicators: permanganate oxidizable C (POXC) and autoclaved citrate-extractable (ACE) protein. Legume residues released the greatest inorganic N, whereas all cover crop residues exhibited a higher soil ACE protein concentration compared to the control without residues. The organic fertilizer released more inorganic N but had a lower ACE protein concentration than manure-based amendments. Grass residues increased POXC relative to sunn hemp, but cover crop residues had no effect on total C. In contrast, manure-based amendments increased soil’s total C but did not affect its POXC. Spinach yield and nutrient uptake were highest with biochar, with no consistent effect of cover crop residues observed on nutrient uptake. Overall, cover crops had the greatest effect on soil health indicators (POXC and ACE protein), whereas manure-based amendments had a greater impact on crop productivity and nutrition (spinach nutrient uptake and yield).
Numerical Investigation of Forced Response in a Transonic Compressor Stage—Highlighting Challenges Using Experimental Validation
Authors: Kilian Nicklas , Klausmann Fabian , Spieker Daniel , Schiffer Heinz-Peter , Salas Mauricio Gutiérrez
Published: June 2024

An experiment-supported simulation process chain is set up to perform numerical forced response analyses on a transonic high-pressure compressor front stage at varying operating conditions. A wake generator is used upstream of the rotor to excite a specific resonance within the operating range of the compressor. Thereby, extensive aerodynamic and structural dynamic experimental data, obtained from state-of-the-art rig testing at the Transonic Compressor Darmstadt test facility at the Technical University of Darmstadt, are used to validate numerical results and ensure realistic boundary conditions. In the course of this, five-hole-probe measurements at steady operating conditions close to the investigated resonance enable a validation of the steady aerodynamics. Subsequently, numerically obtained aeroelastic quantities, such as resonance frequency, and damping, as well as maximum alternating blade stresses and tip deflections, are compared to experimental blade tip timing data. Experimental trends in damping can be confirmed and better explained by considering numerical results regarding the aerodynamic wall work density and secondary flow phenomena. The influence of varying loading conditions on the resonance frequency is not observed as distinctly in numerical, as in experimental results. Generally, alternating blade stresses and deflections appear to be significantly lower than in the experiments. However, similar to the aerodynamic damping, numerical results contribute to a better understanding of experimental trends. The successive experimental validation shows the capabilities of the numerical forced response analysis setup and enables the highlighting of challenges and identification of potential further adaptations.
Susceptibility of Yellow Squash and Zucchini Cultivars to the Sweetpotato Whitefly, Bemisia tabaci Gennadius (MEAM1), in the Southeastern United States
Authors: Mbata George N. , Li Yinping , Warsi Sanower , Simmons Alvin M.
Published: June 2024

The sweetpotato whitefly, Bemisia tabaci (Gennadius) Middle East–Asia Minor 1 (MEAM1), causes significant losses to vegetable crops directly by sap-feeding, inducing plant physiological disorders, and elevating the build-up of sooty mold, and indirectly by transmitting plant viruses. In this study, we evaluated the susceptibility of 20 yellow squash and zucchini (Cucurbita pepo) cultivars to MEAM1, across three growing seasons in the southeastern United States. Weekly sampling of the numbers of MEAM1 adults, nymphs, and eggs were conducted from the fourth week after seed sowing and across 6 weeks during the summer and fall of 2021 and five weeks during the fall of 2022. In general, adult whitefly populations were high during the first week of sampling but decreased as the seasons progressed. The zucchini cultivar ‘Black Beauty’ harbored the most adults, while ‘Green Eclipse Zucchini’ was the least attractive zucchini cultivar to the adults in fall 2022. For yellow squash, ‘Early Summer’ (summer 2021) and ‘Amberpic 8455’ (summer 2021 and fall 2022) were the cultivars with the highest adult populations, while ‘Lioness’ (summer 2021) and ‘Gourmet Gold Hybrid’ (fall 2022) harbored the lowest adult counts. The whitefly egg counts across both vegetables trailed those of adults and peaked in the second week of sampling. The counts of nymphs increased as the seasons progressed, but there was a decline after the second week during fall 2021. For the yellow squash cultivars, ‘Gourmet Gold Hybrid’, (summer 2021 and fall 2022), ‘Lioness’, and ‘Fortune’ (summer 2021) recorded the highest yields. For zucchini, ‘Golden Glory’ (summer 2021) was the top performer. These results provide valuable information for whitefly management in yellow squash and zucchini based on host plant susceptibility and yield.
Chromosome-Level Genome Assembly of Apoderus dimidiatus Voss (Coleoptera: Attelabidae): Insights into Evolution and Behavior
Authors: Xie Meng , Yao Yuhao , Feng Yuling , Xie Lei , Mao Chuyang , He Jinwu , Li Xueyan , Ni Qingyong
Published: June 2024

Attelabidae insects have attracted much attention due to their unique leaf rolling behavior before oviposition. However, the lack of genomic data makes it difficult to understand the molecular mechanism behind their behavior and their evolutionary relationship with other species. To address this gap, we utilized Illumina and Nanopore sequencing platforms along with Hi-C technology to establish a highly accurate whole genome of A. dimidiatus at the chromosome level. The resulting genome size was determined to be 619.26 Mb, with a contig N50 of 50.89 Mb and GC content of 33.89%. Moreover, a total of 12,572 genes were identified, with 82.59% being functionally annotated, and 64.78% designated as repeat sequences. Our subsequent phylogenetic tree analysis revealed that Attelabidae’s divergence from Curculionidae occurred approximately 161.52 million years ago. Furthermore, the genome of A. dimidiatus contained 334 expanded gene families and 1718 contracted gene families. In addition, using Phylogenetic Analysis by Maximum Likelihood (PAML), we identified 106 rapidly evolved genes exhibiting significant signals and 540 positively selected genes. Our research endeavors to serve as an invaluable genomic data resource for the study of Attelabidae, offering fresh perspectives for the exploration of its leaf rolling behavior.
All-on-4 Hybrid with Extra-Long Transnasal Implants: Descriptions of the Technique and Short-Term Outcomes in Three Cases
Authors: Nunes Mariana , de Araújo Nobre Miguel , Camargo Vanderlim
Published: June 2024

Background/Objectives: There is a need for alternative approaches to full-arch rehabilitation of atrophic maxillae. The aim of this short case series was to describe the technique and assess the short term-outcomes of atrophic maxillae rehabilitation using transnasal implants in conjunction with zygomatic implants. Methods: Three female patients (average age: 62 years) presenting comorbidities and atrophic maxillae preventing the insertion of standard maxillary anchored implants received a full-arch fixed prosthesis supported by transnasal implants together with zygomatic implants, using the ad modum all-on-4 concept. Patients were followed during the functional osseointegration period. Primary outcome measures were prosthetic and implant survival based on function. Secondary outcome measures were complication parameters (biological and mechanical), plaque and bleeding levels, and probing pocket depths > 4 mm. Results: No implant failures were registered, and all prostheses remained in function. The only complication was a fracture of a provisional crown that was resolved. Plaque and bleeding scores were mild during the follow-up period. Conclusions: The present manuscript describes the use of extra-long transnasal implants in combination with zygomatic implants in immediate function for full-arch fixed prosthetic rehabilitation of atrophic maxillae, with the objective of promoting more research into this relatively recent technique. More studies are needed to validate the technique.
Recent Advancements in Minimally Invasive Surgery for Early Stage Non-Small Cell Lung Cancer: A Narrative Review
Authors: Khan Jibran Ahmad , Albalkhi Ibrahem , Garatli Sarah , Migliore Marcello
Published: June 2024

Introduction: Lung cancer remains a global health concern, with non-small cell lung cancer (NSCLC) comprising the majority of cases. Early detection of lung cancer has led to an increased number of cases identified in the earlier stages of NSCLC. This required the revaluation of the NSCLC treatment approaches for early stage NSCLC. Methods: We conducted a comprehensive search using multiple databases to identify relevant studies on treatment modalities for early stage NSCLC. Inclusion criteria prioritized, but were not limited to, clinical trials and meta-analyses on surgical approaches to early stage NSCLC conducted from 2021 onwards. Discussion: Minimally invasive approaches, such as VATS and RATS, along with lung resection techniques, including sublobar resection, have emerged as treatments for early stage NSCLC. Ground-glass opacities (GGOs) have shown prognostic significance, especially when analyzing the consolidation/tumor ratio (CTR). There have also been updates on managing GGOs, including the non-surgical approaches, the extent of lung resection indicated, and the level of lymphadenectomy required. Conclusions: The management of early stage NSCLC requires a further assessment of treatment strategies. This includes understanding the required extent of surgical resection, interpreting the significance of GGOs (specifically GGOs with a high CTR), and evaluating the efficacy of alternative therapies. Customized treatment involving surgical and non-surgical interventions is essential for advancing patient care.
Gypsum–Cement–Pozzolan Composites for 3D Printing: Properties and Life Cycle Assessment
Authors: Sahmenko Genadijs , Puzule Liga , Sapata Alise , Slosbergs Peteris , Bumanis Girts , Sinka Maris , Bajare Diana
Published: June 2024

Over the past decade, 3D printing with concrete has been widely adopted worldwide. The primary drivers for this innovation are the reduction in manual labor and the more efficient use of natural resources. New materials that are suitable for 3D printing are developed, which are characterized by rapid setting and robust physical and mechanical properties. In this study, for the first time, ternary gypsum–cement–pozzolanic (GCP) composites were developed and evaluated for use in 3D printing. These composites are associated with durability in water as Portland cement (PC) while maintaining the rapid hardening properties of gypsum. Two types of secondary gypsum—recycled plasterboard gypsum (RG) and phosphogypsum (PG)—were used as the calcium hemihydrate component. The compressive strength test showed that 37 MPa can be achieved, which is comparable to that of traditional PC-based 3D printable mixtures. For the first time in a 3D print test, it was experimentally proved that GCP mixtures have good stability and buildability up to 35 layers. According to Life Cycle Analysis, elaborated material gives a carbon footprint reduction of up to 40%, compared to traditional PC mortar, thus supporting the sustainable use of this innovative composite.
Correction: Klimek et al. A Comparison of the Structure and Selected Mechanical Properties of Cr/Co Alloys Obtained by Casting and Selective Laser Melting. J. Funct. Biomater. 2024, 15, 61
Authors: Klimek Leszek , Bułhak Barbara , Śmielak Beata
Published: June 2024

In the original publication [...]
Practical Formula for Predicting the Residual Deflection Evolution of Steel Plates Subjected to Repeated Impacts
Authors: Huynh Van-Vu , Cho Sang-Rai , Dang Xuan-Phuong , Truong Dac Dung
Published: June 2024

Offshore structures and ships can be progressively damaged due to repeated mass impacts induced by contacts with ships, ice floes, and dropped and/or floating other objects while in service. This paper aims to predict the residual deflection evolution of the marine structures under such impact repetitions. The side hull structures of the general ice-class vessels were selected for this study. The numerical simulations were performed to predict the deflection response of repeatedly impacted stiffened plates by using the software package Abaqus 6.13. For the simulations, the strain hardening of the relevant ice-class steel grade was adopted using the proposed constitutive equations, and the strain-rate hardening effects were taken into account by employing the existing formulations. The developed numerical model was substantiated against tests available in the open literature. Based on the validated model, a parametric study on various stiffened plates was performed. The evolution of the residual deflection of the repeatedly impacted plates with actual scantlings and various impact scenarios was investigated. A practical formula for the prediction of the residual deflection evolution of the plates under repeated mass impacts was proposed based on the regression analysis of the parametric study results. The reliability and accuracy of the proposed formula were confirmed through comparisons with numerical simulations and existing analytical formulations. It is expected that the proposed formula can be efficiently employed as a quick-hand tool for the reliable prediction of the residual deflection evolution incurred by repeated mass impacts.
Biological Control of Root Rot of Strawberry by Bacillus amyloliquefaciens Strains CMS5 and CMR12
Authors: Yang Ruixian , Liu Ping , Ye Wenyu , Chen Yuquan , Wei Daowei , Qiao Cuicui , Zhou Bingyi , Xiao Jingyao
Published: June 2024

Strawberry root rot caused by Fusarium solani is one of the main diseases of strawberries and significantly impacts the yield and quality of strawberry fruit. Biological control is becoming an alternative method for the control of plant diseases to replace or decrease the application of traditional chemical fungicides. To obtain antagonistic bacteria with a high biocontrol effect on strawberry root rot, over 72 rhizosphere bacteria were isolated from the strawberry rhizosphere soil and screened for their antifungal activity against F. solani by dual culture assay. Among them, strains CMS5 and CMR12 showed the strongest inhibitory activity against F. solani (inhibition rate 57.78% and 65.93%, respectively) and exhibited broad-spectrum antifungal activity. According to the phylogenetic tree based on 16S rDNA and gyrB genes, CMS5 and CMR12 were identified as Bacillus amyloliquefaciens. Lipopeptide genes involved in surfactin, iturin, and fengycin biosynthesis were detected in the DNA genomes of CMS5 and CMR12 by PCR amplification. The genes related to the three major lipopeptide metabolites existed in the DNA genome of strains CMS5 and CMR12, and the lipopeptides could inhibit the mycelial growth of F. solani and resulted in distorted hyphae. The inhibitory rates of lipopeptides of CMS5 and CMR12 on the spore germination of F. solani were 61.00% and 42.67%, respectively. The plant-growth-promoting (PGP) traits in vitro screening showed that CMS5 and CMR12 have the ability to fix nitrogen and secreted indoleacetic acid (IAA). In the potting test, the control efficiency of CMS5, CMR12 and CMS5+CMR12 against strawberry root rot were 65.3%, 67.94% and 88.00%, respectively. Furthermore, CMS5 and CMR12 enhanced the resistance of strawberry to F. solani by increasing the activities of defense enzymes MDA, CAT and SOD. Moreover, CMS5 and CMR12 significantly promoted the growth of strawberry seedlings such as root length, seedling length and seedling fresh weight. This study revealed that B. amyloliquefaciens CMS5 and CMR12 have high potential to be used as biocontrol agents to control strawberry root rot.
Spatio-Temporal Dynamics and Drivers of Ecosystem Service Bundles in the Altay Region: Implications for Sustainable Land Management
Authors: Yi Suyan , Wang Hongwei , Xie Ling , Wang Can , Huang Xin
Published: June 2024

Understanding the dynamics of ecosystem services (ESs) in arid landscapes and socio-ecological systems is crucial for sustainable development and human well-being. This study uses the Invest model to quantify the spatio-temporal changes in four key ecosystems services in Altay from 1990 to 2020: water yield (water yield), carbon stock (carbon stock), soil retention (soil retention), and habitat quality (habitat quality). The trade-offs/synergies between different ESs were investigated via Spearman’s correlation analysis. Ecosystem service bundles (ESBs) were mapped using self-organizing mapping (SOM), and the key drivers of ES relationships and the spatio-temporal dynamics of ESBs were revealed through redundancy analysis. The results showed that water yield increased by 33.7% and soil retention increased by 1.2%, while carbon stock and habitat quality decreased by 3.5% and 1.24%, respectively. The spatial distribution pattern had a clear zonal pattern, with the northern mountainous areas higher than the southern desert areas. The six pairs of ESs, in general, showed mainly low trade-off and high synergistic relationships, with trade-offs between water yield and carbon stock, soil retention and habitat quality, and a decreasing trend of trade-offs over time. Four types of ESBs were distinguished, and the compositional differences and spatial distribution within each ESB were determined by interactions between ESs and landscape types. There are complex non-linear relationships between the drivers and the four ESBs in different years. Before 2010, ecological factors were the key drivers influencing the spatio-temporal changes in ESBs, whereas social and environmental factors combined to drive changes in ESB allocations after 2010. Additionally, this study found that the implementation of conservation measures, such as reforestation and sustainable land management practices, positively influenced the provision of ecosystem services in the Altay region. These findings underscore the importance of integrating conservation efforts into land use planning and decision-making processes to ensure the sustainable delivery of ecosystem services in arid landscapes.
New-Onset Hidradenitis Suppurativa in Psoriasis Patients: A Multi-Center, Retrospective Cohort Study
Authors: Li Chen-Pi , Lo Shao-Wei , Tsai Ru-Yin , Chang Hui-Chin , Gau Shuo-Yan
Published: June 2024

Background: Previous research has indicated a potential correlation between hidradenitis suppurativa (HS) and psoriasis (PSO), two chronic inflammatory dermatological diseases. However, there is a lack of comprehensive evaluations that consider a variety of clinical and demographic factors, and the risk of developing HS in PSO patients remains unclear. Our study aims to examine HS risk over time among PSO patients versus matched controls while considering the influence of confounders to provide insights into the potential link between these two diseases. Method: In this multi-institutional cohort study using the TriNetX database, we matched 202,318 patients with PSO with an equivalent number of individuals without PSO, using propensity score matching. The study period extended from 1 January 2005 to 31 December 2018. We computed hazard ratios and their respective 95% confidence intervals (CIs) to evaluate the probability of HS manifestation over a period of 5 years in patients with PSO in comparison to those without PSO. Results: PSO patients demonstrated a consistently higher risk of developing HS than matched controls across all analytic models with the hazard ratios (HR) ranging from 1.43 (95% CI 1.30–1.56) to 5.91 (95% CI 2.49–14.04). Stratified analyses showed the increased HS risk was observed in both genders but only significant in those aged 18–64 years. Kaplan–Meier analysis indicated PSO patients had a higher cumulative probability of developing HS over time (HR 1.77, 95% CI 1.49–1.89). Conclusions: PSO was associated with increased HS risk, highlighting the importance of considering HS as a potential comorbidity in PSO patients and may have implications for early detection, prevention, and management strategies for both conditions. Shared inflammatory pathways, genetic components, and skin dysbiosis may contribute. Further research should elucidate underlying mechanisms.
Exploring New Drug Repurposing Opportunities for MEK Inhibitors in RASopathies: A Comprehensive Review of Safety, Efficacy, and Future Perspectives of Trametinib and Selumetinib
Authors: Gazzin Andrea , Fornari Federico , Cardaropoli Simona , Carli Diana , Tartaglia Marco , Ferrero Giovanni Battista , Mussa Alessandro
Published: June 2024

The RASopathies are a group of syndromes caused by genetic variants that affect the RAS-MAPK signaling pathway, which is essential for cell response to diverse stimuli. These variants functionally converge towards the overactivation of the pathway, leading to various constitutional and mosaic conditions. These syndromes show overlapping though distinct clinical presentations and share congenital heart defects, hypertrophic cardiomyopathy (HCM), and lymphatic dysplasia as major clinical features, with highly variable prevalence and severity. Available treatments have mainly been directed to target the symptoms. However, repurposing MEK inhibitors (MEKis), which were originally developed for cancer treatment, to target evolutive aspects occurring in these disorders is a promising option. Animal models have shown encouraging results in treating various RASopathy manifestations, including HCM and lymphatic abnormalities. Clinical reports have also provided first evidence supporting the effectiveness of MEKi, especially trametinib, in treating life-threatening conditions associated with these disorders. Nevertheless, despite notable improvements, there are adverse events that occur, necessitating careful monitoring. Moreover, there is evidence indicating that multiple pathways can contribute to these disorders, indicating a current need to more accurate understand of the underlying mechanism of the disease to apply an effective targeted therapy. In conclusion, while MEKi holds promise in managing life-threatening complications of RASopathies, dedicated clinical trials are required to establish standardized treatment protocols tailored to take into account the individual needs of each patient and favor a personalized treatment.
Delivery Room Lung Ultrasound—Feasibility, Normal Patterns, and Predictive Value for Respiratory Support in Term and Near-Term Neonates: A Monocentric Study
Authors: Toma Adrian Ioan , Dima Vlad , Fieraru Alina , Arghirescu Alexandra , Andrășoaie Larisa Nicoleta , Chirap Răzvan , Coandă Anelise Alina , Bujdei Teodora , Marinescu Andreea Nicoleta , Isam Al Jashi
Published: June 2024

Aim: our study aimed to characterize the lung ultrasound (LUS) patterns noted immediately after delivery in term and near-term neonates, and to investigate whether the LUS scores or patterns observed at that point could anticipate the need for respiratory support in the sample of patients studied. Materials and methods: We performed two ultrasound examinations: one in the delivery room and the second at one hour of age. The anterior and lateral regions of both lungs were examined. We assessed the correlation between the LUS scores or patterns and the gestational age, umbilical arterial blood gases, the need for respiratory support (CPAP or mechanical ventilation), the presence of respiratory distress, and the need for the administration of oxygen. Results: LUS scores were significantly higher in the delivery room examination (8.05 ± 1.95) than at 1 h of age (6.4 ± 1.75) (p < 0.001). There were also statistically significant differences between the LUS patterns observed in different lung regions between the delivery room exam and the exam performed at 1 h of age (p values between 0.001 and 0.017). There were also differences noted regarding the LUS patterns between different lung regions at the exam in the delivery room (the right anterior region LUS patterns were significantly worse than the right lateral LUS patterns (p < 0.004), left anterior LUS patterns (p < 0.001), and left lateral LUS patterns (p < 0.001)). A statistically significant correlation was found between LUS scores and the gestational age of the patients (r = 0.568, p < 0.001—delivery room; r = 4.0443, p < 0.001—one hour of age). There were statistically significant associations between LUS scores, patterns at delivery (p < 0.001) and 1 h of age (p < 0.001), and the need for respiratory support (CPAP or mechanical ventilation). Conclusions: LUS in the delivery room offers important information regarding lung fluid elimination and aeration of the lungs, and early LUS features are significantly associated with the risk of respiratory distress and the need for respiratory support.
The Effects of Longer Use of Teriparatide on Clinical and Radiographic Outcomes after Spinal Fusion in Geriatric Patients
Authors: Kim Young-Hoon , Ha Kee-Yong , Bae Hyun W. , Park Hyung-Youl , Ko Young-Il , Ko Myung-Sup , Kim Sang-Il
Published: June 2024

Background: Teriparatide is an anabolic agent for osteoporosis and is believed to improve the bone healing process. Previous studies showed that teriparatide could enhance not only fracture healing but also spine fusion. It has been reported that use of teriparatide could promote the spine fusion process and decrease mechanical complications. However, there was no consensus regarding optimal treatment duration. The purpose of this study was to compare surgical outcomes between short-duration and long-duration teriparatide treatment after lumbar fusion surgery in elderly patients. Materials and Methods: All consecutive patients older than 60 years who underwent 1-level lumbar fusion surgery for degenerative diseases between January 2015 and December 2019 were retrospectively reviewed. Based on the duration of teriparatide treatment (daily subcutaneous injection of 20 µg teriparatide), patients were subdivided into two groups: a short-duration (SD) group (<6 months) and a long-duration (LD) group (≥6 months). Mechanical complications, such as screw loosening, cage subsidence, and adjacent vertebral fractures, were investigated. Postoperative 1-year union rate was also evaluated on computed tomography. Clinical outcomes were recorded using visual analog scale (VAS) and Oswestry Disability Index (ODI). Between-group differences for these radiographic and clinical outcomes were analyzed. Results: Ninety-one patients were reviewed in this study, including sixty patients in the SD group and thirty-one patients in the LD group. Their mean age was 72.3 ± 6.2 years, and 79 patients were female. Mean T-score was −3.3 ± 0.8. Cage subsidence (6.7% vs. 3.2%), screw loosening (28.3% vs. 35.5%), and adjacent vertebral fracture (6.7% vs. 9.7%) were not significantly different between the SD and LD groups. Union rate at 1-year postoperative was 65.0% in the SD group and 87.1% in the LD group (p = 0.028). Both groups showed improvement in VAS and ODI after surgery. However, the differences of VAS from preoperative to 6 months and 1 year postoperative were significantly higher in the LD group. Conclusions: Longer teriparatide treatment after lumbar fusion surgery resulted in a higher union rate at 1-year postoperative than the shorter treatment. Also, it could be more beneficial for clinical outcomes.
Immunomodulatory Treatment Impact on IVF Outcomes in KIR AA Genotype: Personalized Fertility Insights
Authors: Seles Luana , Zaha Ioana Alexandra , Luncan Mihai , Bodog Alin , Sachelarie Liliana , Sandor Mircea , Macovei Iulia Codruta , Bimbo-Szuhai Erika , Huniadi Anca
Published: June 2024

Background and Objectives: Recurrent implantation failure (RIF) affects 10% of couples undergoing in vitro fertilization (IVF), spurring exploration into tailored treatments to enhance implantation rates. Maternal immune tolerance towards embryos, particularly killer-cell immunoglobulin-like receptors (KIRs) on natural killer (NK) cells, is a focal point in RIF research. Materials and Methods: This retrospective cohort study, conducted at fertility clinic in Oradea, Romania, involved 65 infertile couples undergoing IVF treatment between January 2022 and December 2023. Couples were divided into two groups: KIR AA (Group A) and KIR Bx (Group B). Results: Factors such as age, type of infertility, oocytes retrieved, embryos produced, pregnancy rates in Group A without and with immunomodulatory treatment were documented. Group A, receiving immunomodulatory treatment, achieved a pregnancy rate of 47.8%, significantly higher than the 23.73% rate without treatment (p = 0.008). Group B had a higher mean patient age than Group A. However, miscarriage rates did not significantly differ between Group A with treatment and Group B (p = 0.2457), suggesting comparable outcomes with immunomodulation. Conclusions: The impact of immunological factors on recurrent implantation failure is being more and more emphasized and warrants the attention of specialists in human reproduction. Uterine natural killers and their function though KIR receptors deserve particular attention as immunomodulatory treatment may improve pregnancy rates in patients with KIR AA haplotype.
Efficient Isolation of Outer Membrane Vesicles (OMVs) Secreted by Gram-Negative Bacteria via a Novel Gradient Filtration Method
Authors: Li Ning , Wu Minghui , Wang Lu , Tang Mengyu , Xin Hongbo , Deng Keyu
Published: June 2024

Bacterial extracellular vesicles (bEVs) secreted by Gram-negative bacteria are referred to as outer membrane vesicles (OMVs) because they originate in the outer membrane. OMVs are membrane-coated vesicles 20–250 nm in size. They contain lipopolysaccharide (LPS), peptidoglycan, proteins, lipids, nucleic acids, and other substances derived from their parent bacteria and participate in the transmission of information to host cells. OMVs have broad prospects in terms of potential application in the fields of adjuvants, vaccines, and drug delivery vehicles. Currently, there remains a lack of efficient and convenient methods to isolate OMVs, which greatly limits OMV-related research. In this study, we developed a fast, convenient, and low-cost gradient filtration method to separate OMVs that can achieve industrial-scale production while maintaining the biological activity of the isolated OMVs. We compared the gradient filtration method with traditional ultracentrifugation to isolate OMVs from probiotic Escherichia coli Nissle 1917 (EcN) bacteria. Then, we used RAW264.7 macrophages as an in vitro model to study the influence on the immune function of EcN-derived OMVs obtained through the gradient filtration method. Our results indicated that EcN-derived OMVs were efficiently isolated using our gradient filtration method. The level of OMV enrichment obtained via our gradient filtration method was about twice as efficient as that achieved through traditional ultracentrifugation. The EcN-derived OMVs enriched through the gradient filtration method were successfully taken up by RAW264.7 macrophages and induced them to secrete pro-inflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukins (ILs) 6 and 1β, as well as anti-inflammatory cytokine IL-10. Furthermore, EcN-derived OMVs induced more anti-inflammatory response (i.e., IL-10) than pro-inflammatory response (i.e., TNF-α, IL-6, and IL-1β). These results were consistent with those reported in the literature. The related literature reported that EcN-derived OMVs obtained through ultracentrifugation could induce stronger anti-inflammatory responses than pro-inflammatory responses in RAW264.7 macrophages. Our simple and novel separation method may therefore have promising prospects in terms of applications involving the study of OMVs.
High-Pressure Torsion: A Path to Refractory High-Entropy Alloys from Elemental Powders
Authors: Mazilkin Andrey , Ferdowsi Mahmoud R. G. , Boltynjuk Evgeniy , Kulagin Roman , Lapovok Rimma
Published: June 2024

For the first time, the refractory high-entropy alloys with equiatomic compositions, HfNbTaTiZr and HfNbTiZr, were synthesized directly from a blend of elemental powders through ten revolutions of high-pressure torsion (HPT) at room temperature. This method has demonstrated its effectiveness and simplicity not only in producing solid bulk materials but also in manufacturing refractory high-entropy alloys (RHEAs). Unlike the melting route, which typically results in predominantly single BCC phase alloys, both systems formed new three-phase alloys. These phases were defined as the Zr-based hcp1 phase, the α-Ti-based hcp2 phase, and the Nb-based bcc phase. The volume fraction of the phases was dependent on the accumulated plastic strain. The thermal stability of the phases was studied by annealing samples at 500 °C for one hour, which resulted in the formation of a mixed structure consisting of the new two hexagonal and cubic phases.