bluuebunny/crossref_metadata_2025 huggingface dataset:
The Endocrine Disruptor Bisphenol A (BPA) Affects the Enteric Neurons Immunoreactive to Neuregulin 1 (NRG1) in the Enteric Nervous System of the Porcine Large Intestine
Authors: Szymańska Kamila , Makowska Krystyna , Całka Jarosław , Gonkowski Sławomir
Published: November 2020

The enteric nervous system (ENS), located in the wall of the gastrointestinal (GI) tract, is characterized by complex organization and a high degree of neurochemical diversity of neurons. One of the less known active neuronal substances found in the enteric neurons is neuregulin 1 (NRG1), a factor known to be involved in the assurance of normal development of the nervous system. During the study, made up using the double immunofluorescence technique, the presence of NRG1 in the ENS of the selected segment of porcine large intestine (caecum, ascending and descending colon) was observed in physiological conditions, as well as under the impact of low and high doses of bisphenol A (BPA) which is commonly used in the production of plastics. In control animals in all types of the enteric plexuses, the percentage of NRG1-positive neurons oscillated around 20% of all neurons. The administration of BPA caused an increase in the number of NRG1-positive neurons in all types of the enteric plexuses and in all segments of the large intestine studied. The most visible changes were noted in the inner submucous plexus of the ascending colon, where in animals treated with high doses of BPA, the percentage of NRG1-positive neurons amounted to above 45% of all neuronal cells. The mechanisms of observed changes are not entirely clear, but probably result from neurotoxic, neurodegenerative and/or proinflammatory activity of BPA and are protective and adaptive in nature.
Transmembrane Polar Relay Drives the Allosteric Regulation for ABCG5/G8 Sterol Transporter
Authors: Xavier Bala M. , Zein Aiman A. , Venes Angelica , Wang Junmei , Lee Jyh-Yeuan
Published: November 2020

The heterodimeric ATP-binding cassette (ABC) sterol transporter, ABCG5/G8, is responsible for the biliary and transintestinal secretion of cholesterol and dietary plant sterols. Missense mutations of ABCG5/G8 can cause sitosterolemia, a loss-of-function disorder characterized by plant sterol accumulation and premature atherosclerosis. A new molecular framework was recently established by a crystal structure of human ABCG5/G8 and reveals a network of polar and charged amino acids in the core of the transmembrane domains, namely, a polar relay. In this study, we utilize genetic variants to dissect the mechanistic role of this transmembrane polar relay in controlling ABCG5/G8 function. We demonstrated a sterol-coupled ATPase activity of ABCG5/G8 by cholesteryl hemisuccinate (CHS), a relatively water-soluble cholesterol memetic, and characterized CHS-coupled ATPase activity of three loss-of-function missense variants, R543S, E146Q, and A540F, which are respectively within, in contact with, and distant from the polar relay. The results established an in vitro phenotype of the loss-of-function and missense mutations of ABCG5/G8, showing significantly impaired ATPase activity and loss of energy sufficient to weaken the signal transmission from the transmembrane domains. Our data provide a biochemical evidence underlying the importance of the polar relay and its network in regulating the catalytic activity of ABCG5/G8 sterol transporter.
The Cutaneous Wound Innate Immunological Microenvironment
Authors: Kirchner Stephen , Lei Vivian , MacLeod Amanda S.
Published: November 2020

The skin represents the first line of defense and innate immune protection against pathogens. Skin normally provides a physical barrier to prevent infection by pathogens; however, wounds, microinjuries, and minor barrier impediments can present open avenues for invasion through the skin. Accordingly, wound repair and protection from invading pathogens are essential processes in successful skin barrier regeneration. To repair and protect wounds, skin promotes the development of a specific and complex immunological microenvironment within and surrounding the disrupted tissue. This immune microenvironment includes both innate and adaptive processes, including immune cell recruitment to the wound and secretion of extracellular factors that can act directly to promote wound closure and wound antimicrobial defense. Recent work has shown that this immune microenvironment also varies according to the specific context of the wound: the microbiome, neuroimmune signaling, environmental effects, and age play roles in altering the innate immune response to wounding. This review will focus on the role of these factors in shaping the cutaneous microenvironment and how this ultimately impacts the immune response to wounding.
Oxidative Stress Orchestrates MAPK and Nitric-Oxide Synthase Signal
Authors: Takata Tsuyoshi , Araki Shoma , Tsuchiya Yukihiro , Watanabe Yasuo
Published: November 2020

Reactive oxygen species (ROS) are not only harmful to cell survival but also essential to cell signaling through cysteine-based redox switches. In fact, ROS triggers the potential activation of mitogen-activated protein kinases (MAPKs). The 90 kDa ribosomal S6 kinase 1 (RSK1), one of the downstream mediators of the MAPK pathway, is implicated in various cellular processes through phosphorylating different substrates. As such, RSK1 associates with and phosphorylates neuronal nitric oxide (NO) synthase (nNOS) at Ser847, leading to a decrease in NO generation. In addition, the RSK1 activity is sensitive to inhibition by reversible cysteine-based redox modification of its Cys223 during oxidative stress. Aside from oxidative stress, nitrosative stress also contributes to cysteine-based redox modification. Thus, the protein kinases such as Ca2+/calmodulin (CaM)-dependent protein kinase I (CaMKI) and II (CaMKII) that phosphorylate nNOS could be potentially regulated by cysteine-based redox modification. In this review, we focus on the role of post-translational modifications in regulating nNOS and nNOS-phosphorylating protein kinases and communication among themselves.
Aerobic Cytotoxicity of Aromatic N-Oxides: The Role of NAD(P)H:Quinone Oxidoreductase (NQO1)
Authors: Nemeikaitė-Čėnienė Aušra , Šarlauskas Jonas , Misevičienė Lina , Marozienė Audronė , Jonušienė Violeta , Lesanavičius Mindaugas , Čėnas Narimantas
Published: November 2020

Derivatives of tirapazamine and other heteroaromatic N-oxides (ArN→O) exhibit tumoricidal, antibacterial, and antiprotozoal activities, which are typically attributed to bioreductive activation and free radical generation. In this work, we aimed to clarify the role of NAD(P)H:quinone oxidoreductase (NQO1) in ArN→O aerobic cytotoxicity. We synthesized 9 representatives of ArN→O with uncharacterized redox properties and examined their single-electron reduction by rat NADPH:cytochrome P-450 reductase (P-450R) and Plasmodium falciparum ferredoxin:NADP+ oxidoreductase (PfFNR), and by rat NQO1. NQO1 catalyzed both redox cycling and the formation of stable reduction products of ArN→O. The reactivity of ArN→O in NQO1-catalyzed reactions did not correlate with the geometric average of their activity towards P-450R- and PfFNR, which was taken for the parameter of their redox cycling efficacy. The cytotoxicity of compounds in murine hepatoma MH22a cells was decreased by antioxidants and the inhibitor of NQO1, dicoumarol. The multiparameter regression analysis of the data of this and a previous study (DOI: 10.3390/ijms20184602) shows that the cytotoxicity of ArN→O (n = 18) in MH22a and human colon carcinoma HCT-116 cells increases with the geometric average of their reactivity towards P-450R and PfFNR, and with their reactivity towards NQO1. These data demonstrate that NQO1 is a potentially important target of action of heteroaromatic N-oxides.
Phylostratic Shift of Whole-Genome Duplications in Normal Mammalian Tissues towards Unicellularity Is Driven by Developmental Bivalent Genes and Reveals a Link to Cancer
Authors: Anatskaya Olga V. , Vinogradov Alexander E. , Vainshelbaum Ninel M. , Giuliani Alessandro , Erenpreisa Jekaterina
Published: November 2020

Tumours were recently revealed to undergo a phylostratic and phenotypic shift to unicellularity. As well, aggressive tumours are characterized by an increased proportion of polyploid cells. In order to investigate a possible shared causation of these two features, we performed a comparative phylostratigraphic analysis of ploidy-related genes, obtained from transcriptomic data for polyploid and diploid human and mouse tissues using pairwise cross-species transcriptome comparison and principal component analysis. Our results indicate that polyploidy shifts the evolutionary age balance of the expressed genes from the late metazoan phylostrata towards the upregulation of unicellular and early metazoan phylostrata. The up-regulation of unicellular metabolic and drug-resistance pathways and the downregulation of pathways related to circadian clock were identified. This evolutionary shift was associated with the enrichment of ploidy with bivalent genes (p < 10−16). The protein interactome of activated bivalent genes revealed the increase of the connectivity of unicellulars and (early) multicellulars, while circadian regulators were depressed. The mutual polyploidy-c-MYC-bivalent genes-associated protein network was organized by gene-hubs engaged in both embryonic development and metastatic cancer including driver (proto)-oncogenes of viral origin. Our data suggest that, in cancer, the atavistic shift goes hand-in-hand with polyploidy and is driven by epigenetic mechanisms impinging on development-related bivalent genes.
Evaluation of Attackers’ Skill Levels in Multi-Stage Attacks
Authors: Mézešová Terézia , Sokol Pavol , Bajtoš Tomáš
Published: November 2020

The rapid move to digitalization and usage of online information systems brings new and evolving threats that organizations must protect themselves from and respond to. Monitoring an organization’s network for malicious activity has become a standard practice together with event and log collection from network hosts. Security operation centers deal with a growing number of alerts raised by intrusion detection systems that process the collected data and monitor networks. The alerts must be processed so that the relevant stakeholders can make informed decisions when responding to situations. Correlation of alerts into more expressive intrusion scenarios is an important tool in reducing false-positive and noisy alerts. In this paper, we propose correlation rules for identifying multi-stage attacks. Another contribution of this paper is a methodology for inferring from an alert the values needed to evaluate the attack in terms of the attacker’s skill level. We present our results on the CSE-CIC-IDS2018 data set.
Influence of Calcium Silicate on the Chemical Properties of Pleurotus ostreatus var. florida (Jacq.) P. Kumm
Authors: Cardoso Rossana V. C. , Carocho Márcio , Fernandes Ângela , Zied Diego Cunha , Cobos Juan Diego Valenzuela , González-Paramás Ana M. , Ferreira Isabel C. F. R. , Barros Lillian
Published: November 2020

Supplementation of mushroom substrates has been linked to a higher resistance against insect pests, although few studies show the impact of this supplementation on the different agronomical parameters of mushrooms or even their chemical composition. In this work, the variation in the biological and chemical composition of oyster mushroom (Pleurotus ostreatus var. florida (Jacq.) P. Kumm) was analysed after varying the substrate supplementation of calcium silicate (0, 0.5, 1, 2, and 4% (w/w%)) during two harvest flushes. Overall, supplementation did not change the weight, the number of fruiting bodies, biological efficiency, yield ratio, and productivity rate of the mushrooms, although the harvest flushes did show significant differences. Furthermore, slight changes were found in the chemical composition with an increase in vitamin D2 and tocopherols for the mushrooms with higher amounts of calcium silicate. Overall, the substrate supplementation did not seem to induce expressive changes or decrease production yields, and can, therefore, continue to be researched as a potential application to fight agronomical pests.
A Hausman Test for Partially Linear Models with an Application to Implied Volatility Surface
Authors: Jiang Yixiao
Published: November 2020

This paper develops a test that helps assess whether the term structure of option implied volatility is constant across different levels of moneyness. The test is based on the Hausman principle of comparing two estimators, one that is efficient but not robust to the deviation being tested, and one that is robust but not as efficient. Distribution of the proposed test statistic is investigated in a general semiparametric setting via the multivariate Delta method. Using recent S&P 500 index traded options data from September 2009 to December 2018, we find that a partially linear model permitting a flexible “volatility smile” and an additive quadratic time effect is a statistically adequate depiction of the implied volatility data for most years. The constancy of implied volatility term structure, in turn, implies that option traders shall feel confident and execute volatility-based strategies using at-the-money options for its high liquidity.
Fundamental Improvement of Creep Resistance of New-Generation Nano-Oxide Strengthened Alloys via Hot Rotary Swaging Consolidation
Authors: Svoboda Jiří , Kunčická Lenka , Luptáková Natália , Weiser Adam , Dymáček Petr
Published: November 2020

New-generation oxide dispersion-strengthened (ODS) alloys with a high volume fraction of nano-oxides of 5% are intended to become the leading creep- and oxidation-resistant alloys for applications at 1100–1300 °C. Hot consolidation of mechanically alloyed powders by intensive plastic deformation followed by heat treatment of the alloys are the key aspects for achieving top creep properties, typically ensured by a coarse-grained microstructure strengthened with homogeneously dispersed, very stable yttrium nano-oxides. The rotary swaging method proves to be favourable for hot consolidation of the new-generation ODS alloy presented. Compared to specimens consolidated by hot rolling, consolidation by hot rotary swaging predetermines the formation of coarse grains with a very high aspect ratio during subsequent secondary recrystallization. Such a grain morphology increases the creep strength of the new-generation ODS alloy considerably.
Influence of MXene (Ti3C2) Phase Addition on the Microstructure and Mechanical Properties of Silicon Nitride Ceramics
Authors: Wozniak Jaroslaw , Petrus Mateusz , Cygan Tomasz , Lachowski Artur , Adamczyk-Cieślak Bogusława , Moszczyńska Dorota , Jastrzębska Agnieszka , Wojciechowski Tomasz , Ziemkowska Wanda , Olszyna Andrzej
Published: November 2020

This paper discusses the influence of Ti3C2 (MXene) addition on silicon nitride and its impact on the microstructure and mechanical properties of the latter. Composites were prepared through powder processing and sintered using the spark plasma sintering (SPS) technic. Relative density, hardness and fracture toughness, were analyzed. The highest fracture toughness at 5.3 MPa·m1/2 and the highest hardness at HV5 2217 were achieved for 0.7 and 2 wt.% Ti3C2, respectively. Moreover, the formation of the Si2N2O phase was observed as a result of both the MXene addition and the preservation of the α-Si3N4→β-Si3N4 phase transformation during the sintering process.
Preparation of Magnesium-Aluminum Hydrotalcite by Mechanochemical Method and Its Application as Heat Stabilizer in poly(vinyl chloride)
Authors: Jiang Yinan , Yang Zhanhong , Su Qingsong , Chen Linlin , Wu Jian , Meng Jinlei
Published: November 2020

The traditional methods for preparing magnesium aluminum layered double hydrotalcite (Mg2Al-CO3LDHs) in industry include coprecipitation and hydrothermal methods. Both these methods have the disadvantages of high preparation cost and complicated water washing process. Using Mg(OH)2, Al(OH)3, and CO2 as raw materials in this work, the Mg2Al-CO3 LDHs are successfully prepared by mechanochemical method, which solves the shortcomings of traditional preparation method and realizes the conversion and utilization of CO2 resource. The prepared Mg2Al-CO3 LDHs are evaluated as a heat stabilizer in poly(vinyl chloride) (PVC). The result indicates that, when 2.4 phr Mg2Al-CO3 LDHs, 0.3 phr ZnSt2, and 0.3 phr of zinc acetylacetonate are added to the PVC, the thermal stability time of PVC can reach 190 min, which is better than PVC containing commercial Mg2Al-CO3 LDHs. Meanwhile, its processing performance is basically the same as the PVC containing commercial Mg2Al-CO3 LDHs.
Modification of Mechanical Properties of Aluminum Alloy Rods via Friction-Extrusion Method
Authors: Wójcicka Anna , Mroczka Krzysztof , Morgiel Jerzy
Published: November 2020

The elaboration of a modified friction-extrusion method aimed at obtaining 2017A aluminum rods of gradient microstructure is described. This was achieved by cutting spiral grooves on the face of the stamp used for alloy extrusion. The experiments were carried out at a constant material feed (~10 mm/min) and a range of tool rotation speeds (80 to 315 rpm). The microstructure observations were carried out using light microscopy (LM) and both scanning and transmission electron microscopy (SEM and TEM). The mechanical properties were assessed through hardness measurements and static tensile tests. The performed investigations show that material simultaneous radial and longitudinal flow, enforced by friction of the rotating tool head and extrusion, results in the formation of two zones of very different microstructures. At the perpendicular section, the outer zone stands out from the core due to circumferential elongation of strings of particles, while in the inner zone the particles are arranged in a more uniform way. Simultaneously, the grain size of the outer zone is refined by two to four times as compared with the inner one. The transfer from the outer zone to the core area is of gradient type. The hardness of the outer zone was found to be ~10% to ~20% higher than that of the core.
Investigation of 2D Rainbow Metamaterials for Broadband Vibration Attenuation
Authors: Meng Han , Chronopoulos Dimitrios , Bailey Nick , Wang Lei
Published: November 2020

Phononic crystals (PnCs) and metamaterials are widely investigated for vibration suppression owing to the bandgaps, within which, wave propagation is prohibited or the attenuation level is above requirements. The application of PnCs and metamaterials is, however, limited by the widths of bandgaps. The recently developed rainbow structures consisting of spatially varied profiles have been shown to generate wider bandgaps than periodic structures. Inspired by this design strategy, rainbow metamaterials composed of nonperiodic mass blocks in two-dimensional (2D) space were proposed in the present study. The blocks were connected by curved beams and tessellated with internal voids to adjust their masses. In order to demonstrate the effects of the rainbow design, two 2D metamaterials, with periodic and nonperiodic units, respectively, were investigated and manufactured using additive manufacturing technologies. Receptance functions, i.e., displacement frequency response functions, of the manufactured metamaterials were calculated with finite element models and measured with a testing system containing a mechanical shaker, an impedance head, and a laser Doppler vibrometer. The obtained numerical and experimental results showed that the metamaterial with rainbow blocks has extended bandgaps compared with the periodic metamaterial.
Polish Transition towards Circular Economy: Materials Management and Implications for the Construction Sector
Authors: Tomaszewska Justyna
Published: November 2020

Poland’s economy as the sixth largest economy in the EU is painfully experiencing the effects of natural resource depletion, which extend to increasing prices and the growing dependence on foreign suppliers. The situation is particularly unfavorable in the construction sector, which is among the most resource- and energy-consuming areas of the economy. This paper juxtaposes the situation of Polish construction industry in the context of the national economy with the context of the evolving EU policies promoting green solutions. The resulting changes in Polish legislation, industry and society are identified. The implementation of selected Circular Economy (CE) aspects, outcomes, emerging challenges and future directions are discussed. The social aspects related to this transformation were analyzed based on a survey carried out among construction industry consumers. The results clearly highlight that individuals are aware of the need to protect the environment, but also indicate a strong necessity to educate the populace about the consequences of the excessive exploitation of the environment and the long-term benefits of CE solutions.
Effects of Sodium Hexametaphosphate Addition on the Dispersion and Hydration of Pure Calcium Aluminate Cement
Authors: Cheng Benjun , Yao Can , Xiong Jian , Liu Xueyin , Zhang Haijun , Zhang Shaowei
Published: November 2020

The effects of sodium hexametaphosphate (SHMP) addition on the dispersion and hydration of calcium aluminate cement were investigated, and the relevant mechanisms discussed. The content of SHMP and the adsorption capacity of SHMP on the surface of cement particles were estimated using plasma adsorption spectroscopy and the residual concentration method. The rheological behavior of hydrate, ζ-potential value of cement particles, phase transformation and the microstructure of the samples were determined by coaxial cylinder rheometer, zeta probe, X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results indicate that SHMP readily reacted with Ca2+, forming complexes [Ca2(PO3)6]2− ions which were subsequently adsorbed onto the surfaces of cement particles. When the content of SHMP was 0.05%, the adsorption ratio reached 99%. However, it decreased to 89% upon further increasing the addition of SHMP to 0.4%. The complexes [Ca2(PO3)6]2− adsorbed onto the surfaces of cement particles inhibited the concentration of Ca2+ and changed ζ-potential, resulting in enhanced electrostatic repulsive force between the cement particles and reduced viscosity of cement-water slurry. The experimental results indicate that the complexes [Ca2(PO3)6]2− covering the surfaces of cement particles led to a delayed hydration reaction, i.e., they extended the hydration time of the cement particles, and that the optimal addition of SHMP was found to be about 0.2%.
Comparative Evaluation of Surface Quality, Tool Wear, and Specific Cutting Energy for Wiper and Conventional Carbide Inserts in Hard Turning of AISI 4340 Alloy Steel
Authors: Abbas Adel T. , Anwar Saqib , Hegab Hussien , Benyahia Faycal , Ali Hazem , Elkaseer Ahmed
Published: November 2020

This paper presents an experimental study into the comparative response of wiper and round-nose conventional carbide inserts coated with TiCN + AL2O3 + TiN when turning an AISI 4340 steel alloy. The optimal process parameters, as identified by pre-experiments, were used for both types of inserts to determine the machined surface quality, tool wear, and specific cutting energy for different cutting lengths. The wiper inserts provided a substantial improvement in the attainable surface quality compared with the results obtained using conventional inserts under optimal cutting conditions for the entire range of the machined lengths. In addition, the conventional inserts showed a dramatic increase in roughness with an increased length of the cut, while the wiper inserts showed only a minor increase for the same length of cut. A scanning electron microscope was used to examine the wear for both types of inserts. Conventional inserts showed higher trends for both the average and maximum flank wear with cutting length compared to the wiper inserts, except for lengths of 200–400 mm, where conventional inserts showed less average flank wear. A higher accumulation of deposited chips was observed on the flank face of the wiper inserts than the conventional inserts. The experimental results demonstrated that edge chipping was the chief tool wear mechanism on the rake face for both types of insert, with more edge chipping observed in the case of the conventional inserts than the wiper inserts, with negligible evidence of crater wear in either case. The wiper inserts were shown to have a higher specific cutting energy than those detected with conventional inserts. This was attributed to (i) the irregular nose feature of the wiper inserts differing from the simpler round nose geometry of the conventional inserts and (ii) a higher tendency of chip accumulation on the wiper inserts.
Chemical and Wetting Analysis of the Ni-Ti Coating on SiC Improved by a 2-Step Coating-Sintering Process
Authors: Liu Yang , Wang Ying-Xin , Yang Qiang , Wang Fu
Published: November 2020

A two-stepped coating-sintering process to prepare the qualified Ni–Ti transition applied in metal-ceramic bonding proved to be effective to improve the wetting abilities. The method was introduced in detail and compared with 2 control groups. To analyze the benefits, the morphology and composition were captured by field emission scanning electron microscopy (FE-SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD). The comparisons of different coating depths and different sintering conditions were also recorded and analyzed. The influence of the EDS detecting depth was a concern in the discussions. Finally, the contact angle tests and surface energies were also estimated to verify the reliability of the transition layer. The results indicated that the coating-sintering process combined with protective sintering was preferred and necessary to increase the activeness.
Numerical Modeling and Analysis of Ti6Al4V Alloy Chip for Biomedical Applications
Authors: Saleem Waqas , Salah Bashir , Velay Xavier , Ahmad Rafiq , Khan Razaullah , Pruncu Catalin I.
Published: November 2020

The influence of cutting forces during the machining of titanium alloys has attained prime attention in selecting the optimal cutting conditions to improve the surface integrity of medical implants and biomedical devices. So far, it has not been easy to explain the chip morphology of Ti6Al4V and the thermo-mechanical interactions involved during the cutting process. This paper investigates the chip configuration of the Ti6Al4V alloy under dry milling conditions at a macro and micro scale by employing the Johnson-Cook material damage model. 2D modeling, numerical milling simulations, and post-processing were conducted using the Abaqus/Explicit commercial software. The uncut chip geometry was modeled with variable thicknesses to accomplish the macro to micro-scale cutting by adapting a trochoidal path. Numerical results, predicted for the cutting reaction forces and shearing zone temperatures, were found in close approximation to experimental ones with minor deviations. Further analyses evaluated the influence of cutting speeds and contact friction coefficients over the chip flow stress, equivalent plastic strain, and chip morphology. The methodology developed can be implemented in resolving the industrial problems in the biomedical sector for predicting the chip morphology of the Ti6Al4V alloy, fracture mechanisms of hard-to-cut materials, and the effects of different cutting parameters on workpiece integrity.
Evaluation of Laboratory Methods of Determination of SBS Content in Polymer-Modified Bitumens
Authors: Ratajczak Maria , Wilmański Artur
Published: November 2020

The article deals with the issue of determination of the content of SBS (Styrene-Butadiene-Styrene) in polymer-modified bitumens (PMBs). The effect of SBS copolymer on the physical and rheological properties of bitumens has been thoroughly investigated and widely described in the literature. Condition surveys of structures and evaluation of the properties of materials used at construction sites have become a huge challenge for construction engineering. Determination of the content of SBS modifier in various building materials (asphalt mixtures and bituminous waterproofing compounds) is a good example in this respect. Based on the laboratory tests, mid-infrared spectroscopy was found to be the most effective analytical method. It can be used for easy detection of the presence of SBS in a modified bitumen. However, quantitative analysis is an issue that calls for research. Currently, there are no standard guidelines, whether national or European, that would regulate the method of testing. Three test methods were assessed in this study: the AASHTO T302–15 standard method and two Australian methods described in codes of practice (T521 and Q350) developed by the local authorities, which define a standard way of determining the amount of SBS in polymer-modified bitumens. The tests were carried out on standard controls and samples sourced from the industry. The above-mentioned test methods were assessed in terms of accuracy of determination, reliability of results obtained on the industrial samples, level of complexity of the test procedure, sample preparation techniques and the type of the required reagents.
Temperature-Dependent Dynamic Characteristics of Carbon-Fiber-Reinforced Plastic for Different Spectral Loading Patterns
Authors: Kim Chan-Jung
Published: November 2020

The dynamic properties of carbon-fiber-reinforced plastic (CFRP) can be efficiently estimated through a modal damping coefficient and a resonance frequency, and the modal parameters can be calculated using a frequency response function (FRF). The modal parameters used in an CFRP FRF are influenced by the carbon fiber direction, temperature, and spectral loading pattern, as well as the operating conditions. In this study, three parameters—temperature, spectral loading pattern, and carbon fiber direction—were selected as the influential factors for CFRP dynamics, and the sensitivity index formulation was derived from the parameter-dependent FRF of the CFRP structure. The derivatives of the parameter-dependent FRF over the three considered parameters were calculated from the measured modal parameters, and the dynamic sensitivity of the CFRP specimens was explored from the sensitivity index results for five different directional CFRP specimens. The acceleration response of a simple CFRP specimen was obtained via a uniaxial excitation test at temperatures ranging from −8 to 105 °C for the following two spectral loading cases: harmonic and random.
Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea
Authors: Gangadhar Katkam N. , Rodrigues Maria João , Pereira Hugo , Gaspar Helena , Malcata F. Xavier , Barreira Luísa , Varela João
Published: November 2020

Tisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report, T. lutea organic crude extracts exhibited in vitro cytotoxic activity against human hepatocarcinoma (HepG2) cells. However, so far, the compound(s) accountable for the observed bioactivity have not been identified. Therefore, the aim of this study was to isolate and identify the chemical component(s) responsible for the bioactivity observed. Bioassay-guided fractionation through a combination of silica-gel column chromatography, followed by preparative thin layer chromatography (PTLC), led to the isolation of two diastereomers of a monoterpenoid lactone, namely, loliolide (1) and epi-loliolide (2), isolated for the first time in this species. The structural elucidation of both compounds was carried out by GC-MS and 1D (1H and 13C APT) and 2D (COSY, HMBC, HSQC-ed, and NOESY) NMR analysis. Both compounds significantly reduced the viability of HepG2 cells and were considerably less toxic towards a non-tumoral murine stromal (S17) cell line, although epi-loliolide was found to be more active than loliolide.
Natural Polypropionates in 1999–2020: An Overview of Chemical and Biological Diversity
Authors: Liu Zhaoming , Liu Hongxin , Zhang Weimin
Published: November 2020

Natural polypropionates (PPs) are a large subgroup of polyketides with diverse structural features and bioactivities. Most of the PPs are discovered from marine organisms including mollusks, fungi and actinomycetes, while some of them are also isolated from terrestrial resources. An increasing number of studies about PPs have been carried out in the past two decades and an updated review is needed. In this current review, we summarize the chemical structures and biological activities of 164 natural PPs reported in 67 research papers from 1999 to 2020. The isolation, structural features and bioactivities of these PPs are discussed in detail. The chemical diversity, bioactive diversity, biodiversity and the relationship between chemical classes and the bioactivities are also concluded.
Molluscan Compounds Provide Drug Leads for the Treatment and Prevention of Respiratory Disease
Authors: Summer Kate , Browne Jessica , Liu Lei , Benkendorff Kirsten
Published: November 2020

Respiratory diseases place an immense burden on global health and there is a compelling need for the discovery of new compounds for therapeutic development. Here, we identify research priorities by critically reviewing pre-clinical and clinical studies using extracts and compounds derived from molluscs, as well as traditional molluscan medicines, used in the treatment of respiratory diseases. We reviewed 97 biomedical articles demonstrating the anti-inflammatory, antimicrobial, anticancer, and immunomodulatory properties of >320 molluscan extracts/compounds with direct relevance to respiratory disease, in addition to others with promising bioactivities yet to be tested in the respiratory context. Of pertinent interest are compounds demonstrating biofilm inhibition/disruption and antiviral activity, as well as synergism with approved antimicrobial and chemotherapeutic agents. At least 100 traditional medicines, incorporating over 300 different mollusc species, have been used to treat respiratory-related illness in cultures worldwide for thousands of years. These medicines provide useful clues for the discovery of bioactive components that likely underpin their continued use. There is particular incentive for investigations into anti-inflammatory compounds, given the extensive application of molluscan traditional medicines for symptoms of inflammation, and shells, which are the principal molluscan product used in these preparations. Overall, there is a need to target research toward specific respiratory disease-related hypotheses, purify bioactive compounds and elucidate their chemical structures, and develop an evidence base for the integration of quality-controlled traditional medicines.
Fucoidan Characterization: Determination of Purity and Physicochemical and Chemical Properties
Authors: Zayed Ahmed , El-Aasr Mona , Ibrahim Abdel-Rahim S. , Ulber Roland
Published: November 2020

Fucoidans are marine sulfated biopolysaccharides that have heterogenous and complicated chemical structures. Various sugar monomers, glycosidic linkages, molecular masses, branching sites, and sulfate ester pattern and content are involved within their backbones. Additionally, sources, downstream processes, and geographical and seasonal factors show potential effects on fucoidan structural characteristics. These characteristics are documented to be highly related to fucoidan potential activities. Therefore, numerous chemical qualitative and quantitative determinations and structural elucidation methods are conducted to characterize fucoidans regarding their physicochemical and chemical features. Characterization of fucoidan polymers is considered a bottleneck for further biological and industrial applications. Consequently, the obtained results may be related to different activities, which could be improved afterward by further functional modifications. The current article highlights the different spectrometric and nonspectrometric methods applied for the characterization of native fucoidans, including degree of purity, sugar monomeric composition, sulfation pattern and content, molecular mass, and glycosidic linkages.
Anti-Inflammatory Cembranoids from a Formosa Soft Coral Sarcophyton cherbonnieri
Authors: Peng Chia-Chi , Huang Chiung-Yao , Ahmed Atallah F. , Hwang Tsong-Long , Sheu Jyh-Horng
Published: November 2020

The present investigation on chemical constituents of the soft coral Sarcophyton cherbonnieri resulted in the isolation of seven new cembranoids, cherbonolides F–L (1–7). The chemical structures of 1–7 were determined by spectroscopic methods, including infrared, one- and two-dimensional (1D and 2D) NMR (COSY, HSQC, HMBC, and NOESY), MS experiments, and a chemical reduction of hydroperoxide by triphenylphosphine. The anti-inflammatory activities of 1–7 against neutrophil proinflammatory responses were evaluated by measuring their inhibitory ability toward N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLF/CB)-induced superoxide anion generation and elastase release in primary human neutrophils. The results showed that all isolates exhibited moderate activities, while cherbonolide G (2) and cherbonolide H (3) displayed a more active effect than others on the inhibition of elastase release (48.2% ± 6.2%) and superoxide anion generation (44.5% ± 4.6%) at 30 µM, respectively.
Diagnostic Performance of Contrast-Enhanced Ultrasound (CEUS) in the Evaluation of Solid Renal Masses
Authors: Geyer Thomas , Schwarze Vincent , Marschner Constantin , Schnitzer Moritz L. , Froelich Matthias F. , Rübenthaler Johannes , Clevert Dirk-André
Published: November 2020

Background: The present study aims to evaluate the diagnostic performance of contrast-enhanced ultrasound (CEUS) for discriminating between benign and malignant solid renal masses. Methods: 18 patients with histopathologically confirmed benign solid renal masses (11 oncocytomas, seven angiomyolipomas) as well as 96 patients with confirmed renal cell carcinoma (RCC) who underwent CEUS followed by radical or partial nephrectomy were included in this single-center study. CEUS examinations were performed by an experienced radiologist (EFSUMB Level 3) and included the application of a second-generation contrast agent. Results: Renal angiomyolipomas, oncocytomas, and renal cell carcinomas showed varying sonomorphological characteristics in CEUS. Angiomyolipomas showed heterogeneous echogenicity (57% hypo-, 43% hyperechoic), while all lesions showed rapid contrast-enhancement with two lesions also showing venous wash-out (29%). Notably, 9/11 oncocytomas could be detected in conventional ultrasound (64% hypo-, 9% hyper-, 9% isoechoic) and 2/11 only demarcated upon intravenous application of contrast agent (18%). All oncocytomas showed hyperenhancement in CEUS, venous wash-out was registered in 7/11 lesions (64%). Conclusions: In line with the current state of knowledge, no specific sonomorphological characteristics allowing for accurate distinction between benign and malignant solid renal masses in CEUS could be detected in our study.
Mini-Review on Lipofuscin and Aging: Focusing on The Molecular Interface, The Biological Recycling Mechanism, Oxidative Stress, and The Gut-Brain Axis Functionality
Authors: Ilie Ovidiu-Dumitru , Ciobica Alin , Riga Sorin , Dhunna Nitasha , McKenna Jack , Mavroudis Ioannis , Doroftei Bogdan , Ciobanu Adela-Magdalena , Riga Dan
Published: November 2020

Intra-lysosomal accumulation of the autofluorescent “residue” known as lipofuscin, which is found within postmitotic cells, remains controversial. Although it was considered a harmless hallmark of aging, its presence is detrimental as it continually accumulates. The latest evidence highlighted that lipofuscin strongly correlates with the excessive production of reactive oxygen species; however, despite this, lipofuscin cannot be removed by the biological recycling mechanisms. The antagonistic effects exerted at the DNA level culminate in a dysregulation of the cell cycle, by inducing a loss of the entire internal environment and abnormal gene(s) expression. Additionally, it appears that a crucial role in the production of reactive oxygen species can be attributed to gut microbiota, due to their ability to shape our behavior and neurodevelopment through their maintenance of the central nervous system.
Investigation into the Novel Microalgae Membrane Bioreactor with Internal Circulating Fluidized Bed for Marine Aquaculture Wastewater Treatment
Authors: Ding Yi , Guo Zhansheng , Mei Junxue , Liang Zhenlin , Li Zhipeng , Hou Xuguang
Published: November 2020

A microalgae membrane bioreactor (MMBR) with internal circulating fluidized bed (ICFB) was constructed at room temperature to study the removal efficiency of marine aquaculture wastewater pollutants and continuously monitor the biomass of microalgae. Within 40 days of operation, the removal efficiency of NO3−–N and NH4+–N in the ICFB-MMBR reached 52% and 85%, respectively, and the removal amount of total nitrogen (TN) reached 16.2 mg/(L·d). In addition, the reactor demonstrated a strong phosphorus removal capacity. The removal efficiency of PO43−–P reached 80%. With the strengthening of internal circulation, the microalgae could be distributed evenly and enriched quickly. The maximum growth rate and biomass concentration reached 60 mg/(L·d) and 1.4 g/L, respectively. The harvesting of microalgae did not significantly affect the nitrogen and phosphorus removal efficiency of ICFB-MMBR. The membrane fouling of the reactor was investigated by monitoring transmembrane pressure difference (TMP). Overall, the membrane fouling cycle of ICFB-MMBR system was more than 40 days.
Influence of Sodium Hypochlorite Treatment on Pore Size Distribution of Polysulfone/Polyvinylpyrrolidone Membranes
Authors: Dibrov George , Kagramanov George , Sudin Vladislav , Grushevenko Evgenia , Yushkin Alexey , Volkov Alexey
Published: November 2020

This work was focused on the study of hypochlorite treatment on the pore size distribution of membranes. To this end, ultrafiltration membranes from a polysulfone/polyvinylpyrrolidone blend with a sponge-like structure were fabricated and exposed to hypochlorite solutions with different active chlorine concentrations for 4 h at ambient temperature. Liquid–liquid displacement and scanning electron microscopy were employed to study the limiting and surface pores, respectively. After treatment with 50 ppm hypochlorite solution at pH = 7.2, a five-fold increase in water permeance up to 1400 L/(m2·h·bar) was observed, accompanied by a 40% increase in the limiting pore sizes and almost a three-fold increase in the porosity. After 5000 ppm treatment at pH = 11.5, a 40% rise in the maximum limiting pore size and almost a two-fold increase in the porosity and permeance was observed, whereas the mean pore size was constant. Apparently, changes in the membrane structure at pH = 11.5 were connected with polyvinylpyrrolidone (PVP) degradation and wash-out, whereas at lower pH and despite lower active chlorine concentration, this process was coupled with polysulfone (PSf) destruction and removal.
Control of Adipose Cell Browning and Its Therapeutic Potential
Authors: Lizcano Fernando , Arroyave Felipe
Published: November 2020

Adipose tissue is the largest endocrine organ in humans and has an important influence on many physiological processes throughout life. An increasing number of studies have described the different phenotypic characteristics of fat cells in adults. Perhaps one of the most important properties of fat cells is their ability to adapt to different environmental and nutritional conditions. Hypothalamic neural circuits receive peripheral signals from temperature, physical activity or nutrients and stimulate the metabolism of white fat cells. During this process, changes in lipid inclusion occur, and the number of mitochondria increases, giving these cells functional properties similar to those of brown fat cells. Recently, beige fat cells have been studied for their potential role in the regulation of obesity and insulin resistance. In this context, it is important to understand the embryonic origin of beige adipocytes, the response of adipocyte to environmental changes or modifications within the body and their ability to transdifferentiate to elucidate the roles of these cells for their potential use in therapeutic strategies for obesity and metabolic diseases. In this review, we discuss the origins of the different fat cells and the possible therapeutic properties of beige fat cells.
In Silico Analysis and In Vitro Characterization of the Bioactive Profile of Three Novel Peptides Identified from 19 kDa α-Zein Sequences of Maize
Authors: Díaz-Gómez Jorge L. , Neundorf Ines , López-Castillo Laura-Margarita , Castorena-Torres Fabiola , Serna-Saldívar Sergio O. , García-Lara Silverio
Published: November 2020

In this study, we characterized three novel peptides derived from the 19 kDa α-zein, and determined their bioactive profile in vitro and developed a structural model in silico. The peptides, 19ZP1, 19ZP2 and 19ZP3, formed α-helical structures and had positive and negative electrostatic potential surfaces (range of −1 to +1). According to the in silico algorithms, the peptides displayed low probabilities for cytotoxicity (≤0.05%), cell penetration (10–33%) and antioxidant activities (9–12.5%). Instead, they displayed a 40% probability for angiotensin-converting enzyme (ACE) inhibitory activity. For in vitro characterization, peptides were synthesized by solid phase synthesis and tested accordingly. We assumed α-helical structures for 19ZP1 and 19ZP2 under hydrophobic conditions. The peptides displayed antioxidant activity and ACE-inhibitory activity, with 19ZP1 being the most active. Our results highlight that the 19 kDa α-zein sequences could be explored as a source of bioactive peptides, and indicate that in silico approaches are useful to predict peptide bioactivities, but more structural analysis is necessary to obtain more accurate data.
In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells
Authors: Kiełbik Aleksander , Szlasa Wojciech , Michel Olga , Szewczyk Anna , Tarek Mounir , Saczko Jolanta , Kulbacka Julita
Published: November 2020

Electroporation, applied as a non-thermal ablation method has proven to be effective for focal prostate treatment. In this study, we performed pre-clinical research, which aims at exploring the specific impact of this so-called calcium electroporation on prostate cancer. First, in an in-vitro study of DU 145 cell lines, microsecond electroporation (μsEP) parameters were optimized. We determined hence the voltage that provides both high permeability and viability of these prostate cancer cells. Subsequently, we compared the effect of μsEP on cells’ viability with and without calcium administration. For high-voltage pulses, the cell death’s mechanism was evaluated using flow-cytometry and confocal laser microscopy. For lower-voltage pulses, the influence of electroporation on prostate cancer cell mobility was studied using scratch assays. Additionally, we applied calcium-binding fluorescence dye (Fluo-8) to observe the calcium uptake dynamic with the fluorescence microscopy. Moreover, the molecular dynamics simulation visualized the process of calcium ions inflow during μsEP. According to our results calcium electroporation significantly decreases the cells viability by promoting apoptosis. Furthermore, our data shows that the application of pulsed electric fields disassembles the actin cytoskeleton and influences the prostate cancer cells’ mobility.