bluuebunny/crossref_metadata_2025 huggingface dataset:
An Efficient Method for Analytically Solving (1+2)-dimensional Chiral Non-linear Schrödinger Equation
Authors: Ozisik Muslum , Bayram Mustafa , Secer Aydin , Cinar Melih
Published: January 2022

In this paper, we have successfully extracted novel analytic solutions for the (1+2)-dimensional Chiral non-linear Schrödinger (NLS) equation by modified extended tanh expansion method combined with new Riccati solutions (METEM-cNRCS) as far as we know. When a wave transformation is applied to the considered Chiral NLS equation, a nonlinear ODE is obtained. Assuming the solutions of ODE have a form as the method suggests, and substituting the trial solutions to the ODE, we get a polynomial. Gathering the coefficients with the same power in the polynomial, we acquire an algebraic equation system. So, we may obtain the abundant solutions of the (1+2)-dimensional Chiral NLS equation by solving the system via Maple. The plots of some solutions are demonstrated to explain the dynamics of the solutions. It is expected that the results of the paper are a guide for future works in traveling wave theory.
QTL Mapping for Pumpkin Fruit Traits Using a GBS-based High-density Genetic Map
Authors: Han Xiaoxia , Min Ziyang , Wei Minghua , Li Yongqi , Wang Duanhua , Zhang Zhuqing , Hu Xinjun , Kong Qiusheng
Published: January 2022

Pumpkin is a popular vegetable crop and exhibits a broad diversity in fruit shape and size. Fruit-related traits are the decisive factors determining consumer acceptance and market value of pumpkin cultivar. As a result, deciphering the genetic basis of fruit-related traits is of great importance for pumpkin breeding. To address this problem, a F2 population was generated by two Cucurbita moschata inbred lines with contrasting fruit shapes and genotyping-by-sequencing approach were used to construct a high-density genetic map and localize the QTLs underlying the fruit-related traits in this study. The results showed that a high-quality genetic map was constructed for pumpkin, which comprised of 2413 bins and spanned a total length of 2252.10 cM with an average genetic distance of 0.94 cM. A total of 30 significant QTLs with moderate or small effects were identified for 7 fruit-related traits, including fruit length, fruit diameter, fruit shape index, fruit weight, fruit flesh thickness, seed cavity size, and total soluble solids content. Co-locations and close locations were observed between the QTLs underlying different traits, demonstrating that pleiotropic effect plays an important role in genetic control of fruit-related traits. The identified QTLs provide valuable information for further fine mapping of the related genes and pumpkin breeding programs with the aim of improving fruit quality.
Outcomes From a Hybrid Implementation-effectiveness Study of the Living Well During Pregnancy Tele-coaching Program for Women at High Risk of Excessive Gestational Weight Gain
Authors: de Jersey Susan , Meloncelli Nina , Guthrie Taylor , Powlesland Hilary , Callaway Leonie , Chang Angela T , Wilkinson Shelley , Comans Tracy , Eakin Elizabeth
Published: January 2022

BackgroundExcess gestational weight gain (GWG) is associated with short-term perinatal complications and longer term cardiometabolic risks for mothers and their babies. Dietitian counselling and weight gain monitoring for women at risk of high pregnancy weight gain is recommended by clinical practice guidelines. However, face-to-face appointments, during a time with high appointment burden, can introduce barriers to engaging with care. Telephone counselling may offer a solution. The Living Well during Pregnancy (LWdP) program is a dietitian-delivered telephone coaching program implemented within routine antenatal care for women at risk of excess GWG. This program evaluation used a hybrid implementation-effectiveness design guided by the RE-AIM framework reports on the primary outcomes (reach, adoption, implementation, maintenance) and secondary outcomes (effectiveness) of the LWdP intervention. MethodsThe LWdP program evaluation compared data from women participating in the LWdP program with a historical comparison group (pregnant women receiving dietetic counselling for GWG in the 12 months prior to the study). The primary outcomes were described for the LWdP program. Between group comparisons were used to determine effectiveness of achieving appropriate GWG and pre and post intervention comparisons of LWdP participants was used to determine changes to dietary intake and physical activity. ResultsThe LWdP intervention group (n=142) were compared with women in the historical comparison group (n=49). Women in the LWdP intervention group attended 3.4 (95% CI 2.9 – 3.8) appointments compared with 1.9 (95% CI, 1.6 – 2.2). GWG was similar between the two groups, including the proportion of women gaining weight above the Institute of Medicine recommendations (70% vs 73%, p=0.69). Within group comparison showed that total diet quality, intake of fruit and vegetables and weekly physical activity were all significantly improved from baseline to follow-up for the women in the LWdP, while consumption of discretionary food and time spent being sedentary decreased (all p<0.05). ConclusionThe LWdP program resulted in more women accessing care and positive improvements in diet quality, intuitive eating behaviours and physical activity. It was as effective as face-to-face appointments for GWG, though more research is required to identify how to engage women earlier in pregnancy and reduce appointment burden.
Bus Travel Time Reliability Incorporating Stop Waiting Time and In-vehicle Travel Time with AVL Data
Authors: Zhuang Zixu , Cheng Zhanhong , Yao Jia , Wang Jian , An Shi
Published: January 2022

Improving bus operation quality can attract more commuters to use bus transit, and therefore reduces the share of car and alleviates traffic congestion. One important index of bus operation quality is the bus travel time reliability, which in this paper is defined to be the probability when the sum of bus stop waiting time and in-vehicle travel time is less than a certain threshold. We formulate the bus travel time reliability by the convolution of independent events’ probabilities, and elaborate the calculation method using Automatic Vehicle Location (AVL) data. Next, the No.63 Bus Line in Harbin City is used to test the applicability of the proposed method, and analyze the influence factors of the bus travel time reliability. The numerical results show that factors such as weather, workday, departure time, travel distance, and the distance from the boarding stop to the bus departure station will significantly affect the travel time reliability. At last, some general conclusions and future research are summarized.
PARG Suppresses Tumorigenesis and Downregulates Genes Controlling Angiogenesis, Inflammatory Response, and Immune Cell Recruitment
Authors: Johnson Sarah , Karpova Yaroslava , Guo Danping , Ghatak Atreyi , Markov Dmitriy A. , Tulin Alexei V.
Published: January 2022

Chemokines are highly expressed in tumor microenvironment and play a critical role in all aspects of tumorigenesis, including the recruitment of tumor-promoting immune cells, activation of cancer-associated fibroblasts, angiogenesis, metastasis, and growth. Poly(ADP-ribose) polymerase (PARP) is a multi-target transcription regulator with high levels of poly(ADP-ribose) (pADPr) being reported in a variety of cancers. Furthermore, poly(ADP-ribose) glycohydrolase (PARG), an enzyme that degrades pADPr, has been reported to be downregulated in tumor tissues with abnormally high levels of pADPr. In conjunction to this, we have recently reported that the reduction of pADPr, by either pharmacological inhibition of PARP or PARG’s overexpression, disrupts renal carcinoma cell malignancy in vitro. Here, we use 3T3 mouse embryonic fibroblasts, a universal model for malignant transformation, to follow the effect of PARG upregulation on cells’ tumorigenicity in vivo. We found that the overexpression of PARG in mouse allografts produces significantly smaller tumors with a delay in tumor onset. As downregulation of PARG has also been implicated in promoting the activation of pro-inflammatory genes, we also followed the gene expression profile of PARG-overexpressing 3T3 cells using RNA-seq approach and observed that chemokine transcripts are significantly reduced in those cells. Our data suggest that the upregulation of PARG may be potentially useful for the tumor growth inhibition in cancer treatment and as anti-inflammatory intervention.
IGF-1 Conjugated Sub-5 nm Ultrafine Iron Oxide Nanoparticle Enhances Targeted Drug Delivery and Efficacy of DNA Topoisomerase Inhibitor SN38 for Pancreatic Cancer: An in Vitro Study
Authors: Xu Peijia , Xue Ting , Padelford Jonathan , Xue Xingkui , Wu Alyssa Y , Li Yuancheng , Wang Liya
Published: January 2022

Background

Pancreatic cancer remains one of the most lethal cancers largely due to the inefficient delivery of therapeutics. Nanomaterials have been extensively investigated as drug delivery platforms, showing improved drug pharmacodynamics and pharmacokinetics. However, their applications in pancreatic cancer have not yet been successful due to limited tumor delivery caused by dense tumor stroma and distorted tumor vasculatures. Meanwhile, smaller-sized nanomaterials have shown improved tumor delivery and retention in various tumors, including pancreatic tumors, suggesting their potential in enhancing drug delivery.
Methods

An ultrafine iron oxide nanoparticle (uIONP) was used to encapsulate 7-ethyl-10-hydroxyl camptothecin (SN38), the water-insoluble active metabolite of chemotherapy drug irinotecan for treating pancreatic cancer in clinic. Insulin-like growth factor 1 (IGF-1) was conjugated to uIONP as a ligand for targeting pancreatic cancer and stromal cells overexpressing IGF-1 receptor (IGF1R). The SN38 loading and release profile were characterized. The cancer cell targeting and induced apoptosis by developed nano-formulationIGF1-uIONP/SN38 were also investigated.
Results

IGF1-uIONP/SN38 demonstrated stable drug loading in physiological pH with the loading efficiency of 68.2 ± 3.5% (SN38/Fe, wt%) and <7% release for 24 hours. In tumor-interstitial- and lysosomal-mimicking pH (6.5 and 5.5), 52.2 and 91.3% of encapsulated SN38 were released over 24 hours. The IGF1-uIONP/SN38 exhibited specific receptor-mediated cell targeting and cytotoxicity to MiaPaCa-2 cells with IC50 of 11.8 ± 2.3 nM, but not to HEK293 human embryonic kidney cells.
Conclusion

The IGF1-uIONP significantly improved the delivery of SN38 to targeted pancreatic cancer cells, holding the potential for in vivo theranostic applications.
Association of Rs13118928 and Rs1828591 Polymorphisms in HHIP Gene with COPD Susceptibility: A Meta-Analysis of Case-Control Studies
Authors: Wei Xue-mei , Yang Xiao-hong
Published: January 2022

In recent years, investigators have been striving to explore the pathogenesis of chronic obstructive pneumonia disease (COPD). Hedgehog Interacting Protein (HHIP) has been identified as a candidate susceptibility gene. Our aim is to synthesize and include all evidences to get a more comprehensive result. We searched 6 online databases- PubMed, Web of Science, Cochrane Library, Wanfang, EMBASE, CNKI. All included studies were published before October 1, 2021. We used Newcastle-Ottawa scale (NOS) to evaluate the bias of each study. Meta-analysis methods were conducted to evaluate the pooled result. A total of 14 comparative studies were included in this meta-analysis, for rs13118928 polymorphism, significant associations were observed in 5 genetic models, (A vs. G, OR=1.18, 95CI%=[1.07-1.30], P=0.0006; AA vs. GG, OR=1.56, 95CI%=[1.22-2.00], P=0.0004; AG vs. GG, OR=1.28, 95CI%=[1.05-1.55], P=0.01; AA+AG vs. GG, OR=1.36, 95CI%=[1.12-1.65], P=0.002; AA vs. AG+GG, OR=1.18, 95CI%=[1.05-1.33], P=0.006). as for rs1828591, there were also significant associations detected in the overall population, (A vs. G, OR=1.12, 95CI%=[1.05-1.19], P=0.0003; AA vs. GG, OR=1.27, 95CI%=[1.04-1.56], P=0.02; AG vs. GG, OR=1.25, 95CI%=[1.03-1.51], P=0.02; AA+AG vs. GG, OR=1.26, 95CI%=[1.04-1.53], P=0.02; AA vs. AG+GG, OR=1.10, 95CI%=[1.01-1.19], P=0.03). This meta-analysis showed that the A allele in both rs13118928 and rs1828591 was turn out to be the risk allele in developing COPD. The result of Codominant genetic model, Dominant genetic model and Recessive genetic model remain the same.
Barriers to and Facilitators of Return to Work in Head and Neck Cancer Patients with in the First Six Months Post-Treatment
Authors: Huang Bing-Shen , Lin Chien-Yu , Chang Ya-Lan , Chung Ching-Fang , Chen Shu-Ching
Published: January 2022

Objective

To identify the factors associated with barriers to and facilitators of return to work (RTW) in head and neck cancer (HNC) patients in the first six months post-treatment.
Methods

This cross-sectional study examined HNC patients who completed treatment from the outpatient radiation department of a single cancer center in northern Taiwan from October 2018 to July 2020. Demographic and clinical characteristics were recorded, and patients were assessed using the Return to Work Barrier Scale, Return to Work Facilitator Scale, Distress Thermometer, Numeric Rating Scale, and Karnofsky Performance Status Scale questionnaires.
Results

Of the 106 HNC patients surveyed, 54.7% successfully RTW. Barriers to RTW included patient-perceived worst health status, greater symptom burden, and age ≥55 years. These factors explained 34.6% of the variance in overall barriers to RTW. Facilitators of RTW were lower psychological distress and who did not receive reconstruction surgery. These factors explained 17.9% of the variance in facilitators to RTW.
Conclusion

Patient-perceived health status and age most strongly influence RTW in HNC patients. HNC patients who can RTW should be encouraged to do so, and clinician awareness of potential barriers can aid patients in their RTW.
Predictors of Migration in an HIV Hyper-Endemic Rural South African Community: Evidence From a Population-Based Cohort (2005-2017)
Authors: Dzomba Armstrong , Kim Hae-Young , Tomita Andrew , Vandormael Alain , Govender Kaymarlin , Tanser Frank
Published: January 2022

Globally, South Africa ranks worst for people living with HIV (PLHIV) and the unique legacy of internal labour migration continues to be a major driver of the regional epidemic, interrupting treatment-as-prevention efforts. The study examined levels, trends, and predictors of migration in rural KwaZulu-Natal Province, South Africa, using population-based surveillance data from 2005 through 2017. We followed 69 604 adult participants aged 15-49 years and recorded their migration events (i.e., out-migration from the surveillance area) in 423 038 person-years over 525 397 observations. Multiple failure Cox-regression models were used to measure the risk of migration by socio-demographic factors: age, sex, educational status, marital status, HIV, and community antiretroviral therapy (ART) coverage. Overall, 69% of the population cohort experienced at least one migration event during the follow-up period. The average incidence rate of migration was 9.96 events and 13.23 events per 100 person-years in women and men, respectively. Migration rates declined from 2005 to 2008 then peaked in 2012 for both women and men. Adjusting for other covariates, the risk of migration was 3.4-times higher among young women aged 20-24 years compared to those aged ≥40 years (adjusted Hazard Ratio [aHR]=3.37, 95% Confidence Interval [CI]: 3:19-3.57), and 2.9-times higher among young men aged 20-24 years compared to those aged ≥40 years (aHR=2.86, 95% CI:2.69-3.04). There was a 9% and 27% decrease in risk of migration among both women (aHR=0.91, 95% CI: 0.83 – 0.99) and men (aHR=0.73, 95% CI 0.66 – 0.82) respectively per every 1% increase in community ART coverage. Young unmarried women including those living with HIV, migrated at a magnitude similar to that of their male counterparts, and lowered as ART coverage increased over time, reflecting the role of improved HIV services across space in reducing out-migration. A deeper understanding of the characteristics of a migrating population provides critical information towards identifying and addressing gaps in the HIV prevention and care continuum in an era of high mobility.
The Impact of COVID-19 On Emergency Visits For Back And Neck Pain
Authors: Ohana Nissim , Engel Itzhak , Baruch Yuval , Benharroch Daniel , Sheinis Dimitri
Published: January 2022

Purpose: To assess the rate of visits to the emergency department of our medical center concerning low back or neck pain, as a factor of COVID-19 confinements.Methods: The study period was a 30-weeks interval during the COVID-19 pandemic contrasted by a similar stretch in the year preceding the epidemic. The visits to the Emergency Department, prompted by low back or neck pain, were recorded prior and during lockdowns of the pandemic. The significance of the confinements for the development of the pain syndromes was evaluated.Results: A total of 1530 patients with newly diagnosed back or neck pain were enrolled. Most patients visited our emergency department for low back pain, commonly those older than 60 years. No significant gender variance was disclosed, though most visits of females were for low back pain. Low back pain presentations were curbed following confinement, but the rate of stays for neck pain had swelled by more than 10%. Despite back pain predominance, visits for neck pain persisted. Before COVID-19, the average weekly number of Emergency Department visits was 38.5. This was followed by sharp drops during COVID-19 lockdown (mean different=-22.2, 95%CI=-28.7, -15.7, p<0.001) (not significant).Conclusions: COVID-19 lockdowns have a significant impact on the Emergency Department’s presentations due to back and neck pain. A higher rate of presentation for back pain compared to neck pain is probably related to COVID-19, without being affected directly by the SARS-CoV-2: confinement-induced immobility might instigate musculoskeletal sequels, which may be attributed to stress or other psycho-social afflictions.
Quality Control of Mitochondria in Parkinson's Disease (PD) Using ATP And Bradford Assays
Authors: Akram Al-Baraa
Published: January 2022

Parkinson's disease is a heterogeneous, multifactorial and often complex disease characterized by motor impairment due to the presence of Lewy bodies and prominent degeneration of dopaminergic neurons in the substantia nigra. Although the specific pathogenesis involving PD remains under investigation, mitochondrial dysfunction has been widely accepted as one of the major pathogenic pathways underlying the development of PD. Based on the hypothesis that depiction of HtrA2 (serine protease gene, mitochondrial precursor) might contribute to an increase in mitochondrial stress and transcriptional upregulation of the nuclear stress-response CHOP gene. The present study aimed to analyze through laboratory-based research the role of HtrA2 and CHOP in the transmission of stress signaling and the consequent activation of mitochondrial quality control in Parkinson's disease using ATP and Bradford assays.
Exploring Influencing Factors of Chronic Obstructive Pulmonary Disease Based on Elastic Net and Bayesian Network
Authors: Quan Dichen , Ren Jiahui , Ren Hao , Linghu Liqin , Wang Xuchun , Li Meichen , Qiao Yuchao , Ren Zeping , Qiu Lixia
Published: January 2022

This study aimed to construct Bayesian networks(BNs) to analyze the network relationship between those influencing factors and COPD, and to explore their intensity of effect on COPD through network reasoning. Elastic Net and Max-Min Hill-Climbing(MMHC) hybrid algorithm were adopted to screen the variables on the monitoring data of COPD among residents in Shanxi Province, China from 2014 to 2015, and construct BNs respectively. After variables selection by Elastic Net, 10 variables closely related to COPD were selected finally. The BNs constructed by MMHC showed that smoking status, household air pollution, family history, cough, air hunger or dyspnea were directly related to COPD, and Gender was indirectly linked to COPD through smoking status. Moreover, smoking status, household air pollution and family history were the parent nodes of COPD, and cough, air hunger or dyspnea represented the child nodes of COPD. In other words, smoking status, household air pollution and family history were related to the occurrence of COPD, and COPD would make patients’ cough, air hunger or dyspnea worse. Generally speaking, BNs could reveal the complex network relationship between COPD and its relevant factors well, making it more convenient to carry out targeted prevention and control of COPD.
An efficient 2-aminothiazolesalicylaldehyde Fluorescent Chemosensor for Fe2+ ion Detection and Potential Inhibitor of NUDT5 Signaling Hormone for Breast Cancer Cell and Molecular Keypad Lock Application
Authors: Basha Summaya Banu , Charles Immanuel David , Raju Nandhakumar , Manokaran Sakthivel , Kuzhandaivel Hemalatha
Published: January 2022

A novel thiazole phenol conjugate 2-aminothiazolesalicylaldehyde (receptor1) was designed and synthesized for the first time through a single step process via schiff base condensation reaction. The formation of receptor1 was confirmed by FTIR, 13C NMR and 1H NMR. The receptor1 complexing with various metal ions studied through fluorescence spectroscopy showed its selectivity towards Fe2+ ion following a reverse photo induced electron transfer (PET) process compared to all other potentially competing ions. Furthermore, the receptor1 showed excellent reversibility property on adding EDTA solution. The binding affinity between receptor1-Fe2+ ion was found to be Ka = 2.22x102 M-1 and the sensing affinity of receptor1 towards Fe2+ ion at nanolevel concentration was 33.7x10-9 M which is lower than the detection limit of existing Schiff base. The receptor1 was used to detect Fe2+ ion in different water samples. Using molecular docking the chelating function of ligand (receptor1) was assessed as a potential inhibitor of NUDT5, a silence hormone signaling for breast cancer. The excellent reversibility property is used to construct INHIBIT molecular logic gate.
Mesothelin-Specific T Cell Cytotoxicity Against Triple Negative Breast Cancer Is Enhanced By 40s Ribosomal Protein Subunit 3-Treated Self-Differentiated Dendritic Cells
Authors: Jirapongwattana Niphat , Thongchot Suyanee , Chiraphapphaiboon Wannasiri , Chieochansin Thaweesak , Sa-nguanraksa Doonyapat , Warnnissorn Malee , Thuwajit Peti , Yenchitsomanus Pa-Thai , Thuwajit Chanitra
Published: January 2022

Purpose Triple negative breast cancer (TNBC) is deficient in targeted treatment resulting in poor prognosis. Targeting overexpressed mesothelin (MSLN) using MSLN-specific T cells is an attractive treatment approach.Methods The immunohistochemistry of MSLN in TNBC tissues were performed. A lentiviral vector harboring granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and MSLN cDNAs was constructed to generate self-differentiated myeloid-derived antigen-presenting-cells reactive against tumor expressing MSLN dendritic cells (MSLN-SmartDC) for MSLN-specific T cell activation. The antigen specificity and cancer killing of activated T cells were accessed.Results The high expression of MSLN was found in 32.8% all breast cancer subtypes and 57% in TNBC. High MSLN was significantly associated with the TNBC subtype and the absence of ER, PR and HER2. MSLN-SmartDC exhibited comparable phenotype to DC generated by exogenous cytokine treatment; addition of 40s ribosomal protein subunit 3 (RPS3), a toll-like receptor 4 ligand, enhanced DC maturation and function by upregulation of CD40, CD80 and CD83 expression and IL-12p70 secretion. MSLN-specific CD8+CD69+ IFN-γ+ T cells were detected in T cells activated by both MSLN-SmartDC and RPS3-MSLN-SmartDC. MSLN-specific T cells activated by these DCs showed more specific killing capability against naturally expressed MSLN-HCC70 and artificially MSLN-overexpressing MDA-MB-231 compared to parental MDA-MB-231 in both 2 dimensional (2D)- and 3D-culture systems. Conclusion High MSLN was observed in TNBC patients, a potential target for TNBC treatment. MSLN-SmartDC could promote MSLN-specific T cell response against TNBC and RPS3 can enhance the cytolytic activity of these T cells providing an alternative treatment approach for TNBC patients.
How to Anticipate Public Health Indicators Using Wastewater SARS-CoV-2 Concentration During the COVID-19 Pandemic. Madrid Region Case Study
Authors: Lastra Antonio , Botello Jaime , Pinilla Alejandro , Canora Jesús , Sánchez Juan , Urrutia Iñaki , Fernández Pascual , Zapatero Antonio , Candel Francisco Javier , Ortega Mónica , Ortega Celia
Published: January 2022

This study analyzes the presence and dynamics of SARS-CoV-2 in sewage sanitation systems in the Madrid region. The statistical results and data generated are presented daily via a platform as a tool for the early detection of SARS-CoV-2 and its spread based on the WBE (wastewater-based epidemiological) approach. The number of sampled points amounts to a total of 289 sampling points in terms of SARS-CoV-2 concentration that collects over six million and a half inhabitants’ discharges. The project was developed by Canal de Isabel II, the water utility company from Madrid. The research evaluates the correlation found between SARS-CoV-2 concentration in wastewater and the following public health indicators: incidence rate, reported active cases and COVID-19 hospitalization data (regular hospitalization and ICU admission cases).SARS-CoV-2 presence and dynamics in wastewater show a strong connection with both 14-day incidence rates with active infection and reported COVID-19 hospitalizations. A lag varying from 3 to 8 days between wastewater presence and hospitalizations is explained because the infection is found in the feces of patients before symptom onset. The resulting data are available for consultancy on the company’s website (named VIGÍA project) as well as on the regional government’s websites.The results have already been useful to anticipate the second and third COVID-19 waves in Madrid. Information is shared daily with health authorities for consultancy and decision-making. The results are available as an aggregation for the entire region and for each sewershed.
Analysis of Sustainability In Livestock Production Systems In Cordoba, Colombia.
Authors: Nobles Yhonattan Mendez , Rocha Alfonso Acevedo , Farreras Jordi Morató , Manchego Katy Pacheco , Padilla Sharon Martínez , Lorduy Jorge Villadiego
Published: January 2022

The concept of sustainability is usually broad; it refers to the ability of the system to meet the current demand for livestock products without compromising the use of resources by future generations. Therefore, this study has been conducted to provide an overview of rotational, intensive, and regenerative livestock models to evaluate and measure their level of sustainability in the environmental, social, and economic dimensions to guide livestock guilds, professionals and stakeholders to transform their agricultural systems by adopting the concept of sustainability. The results show that the regenerative livestock system has a high association with the economic dimension in the Almost Good category, in the environmental dimension in the Moderate category, and in the social dimension in the Almost Good and Very Good categories. On the other hand, the rotational livestock system and the intensive livestock system have an association with the environmental dimension categorized as good; however, the association of the intensive livestock system is not as strong as that of the rotational livestock system.
COVID-19 Vaccine Perceptions in the Initial Phases of US Vaccine Roll-out: An Observational Study on Reddit
Authors: Kumar Navin , Corpus Isabel , Hans Meher , Harle Nikhil , Yang Nan , McDonald Curtis , Sakai Shinpei Nakamura , Janmohamed Kamila , Chen Keyu , Altice Frederick L. , Tang Weiming , Schwartz Jason L. , Jones-Jang S. Mo , Saha Koustuv , Memon Shahan Ali , Bauch Chris T. , De Choudhury Munmun , Papakyriakopoulos Orestis , Tucker Joseph D. , Goyal Abhay , Tyagi Aman , Khoshnood Kaveh , Omer Saad
Published: January 2022

Background: Open online forums like Reddit provide an opportunity to quantitatively examine COVID-19 vaccine perceptions early in the vaccine timeline. We examine COVID-19 misinformation on Reddit following vaccine scientific announcements, in the initial phases of the vaccine timeline. Methods: We collected all posts on Reddit from January 1 2020 - December 14 2020 (n=266,840) that contained both COVID-19 and vaccine-related keywords. We used topic modeling to understand changes in word prevalence within topics after the release of vaccine trial data. Social network analysis was also conducted to determine the relationship between Reddit communities (subreddits) that shared COVID-19 vaccine posts, and the movement of posts between subreddits. Results: There was an association between a Pfizer press release reporting 90\% efficacy and increased discussion on vaccine misinformation. We observed an association between Johnson and Johnson temporarily halting its vaccine trials and reduced misinformation. We found that information skeptical of vaccination was first posted in a subreddit (r/Coronavirus) which favored accurate information and then reposted in subreddits associated with antivaccine beliefs and conspiracy theories (e.g. conspiracy, NoNewNormal). Conclusions: Our findings can inform the development of interventions where individuals determine the accuracy of vaccine information, and communications campaigns to improve COVID-19 vaccine perceptions, early in the vaccine timeline. Such efforts can increase individual- and population-level awareness of accurate and scientifically sound information regarding vaccines and thereby improve attitudes about vaccines, especially in the early phases of vaccine roll-out. Further research is needed to understand how social media can contribute to COVID-19 vaccination services.
Efficacy of Complete Laparoscopic Ileal Augmentation Cystoplasty for the Treatment of Low Bladder Capacity and Compliance: a Case Series
Authors: Yang Chao , Chen Xin , Wang Yi , Fang Lu , Sun Wei , Bi Liangkuan , Yu Dexin
Published: January 2022

Objectives: To investigate the safety and efficacy of complete laparoscopic ileal augmentation cystoplasty for the treatment of low bladder capacity and compliance.Methods: The clinical data of 13 patients with low bladder capacity and compliance were retrospectively analyzed. Therapeutic efficacy was evaluated at follow-up. The Clavien system was used to evaluate the severity of postoperative complications.Results: All 13 operations were successfully completed laparoscopically. The operation duration was 140-248 min (average: 189.9 ± 29.6 min), the time to postoperative recovery of bowel function was 1-10 days (average: 2.9 ± 2.3 days). There were 4 cases of grade I complications and 1 case of grade II complications (i.e., paralytic ileus caused by urinary leakage from the anastomosis of the augmented bladder). Cystography showed that the morphology of the bladder was close to normal, and the maximum safe capacity and compliance of the bladder were significantly increased [103.8 ± 16.6 mL and 332.3 ± 20.5 mL, p < 0.01; 7.0 ± 1.3 mL/cm H2O and 32.4 ± 2.1 mL/cm H2O, p < 0.01]. All patients were able to urinate spontaneously after catheter removal.Conclusions: Complete laparoscopic ileal augmentation cystoplasty is a safe and feasible treatment for low bladder capacity and compliance, and has the advantages of less trauma, less bleeding, faster recovery of intestinal function, and fewer postoperative complications. This treatment effectively increases bladder capacity, protects upper urinary tract function, and improves patient quality of life, and thus warrants clinical application.
Palliative Radiotherapy for Gastric Cancer Bleeding: A Multi-Institutional Retrospective Study.
Authors: Takeda Kazuya , Sakayauchi Toru , Kubozono Masaki , Katagiri Yu , Umezawa Rei , Yamamoto Takaya , Ishikawa Yojiro , Takahashi Noriyoshi , Suzuki Yu , Kishida Keita , Jingu Keiichi
Published: January 2022

Background: Palliative radiotherapy for gastric cancer bleeding has been reported to be a safe and effective treatment, but predictive factors for achievement of hemostasis and overall survival have not been established.Methods: In this retrospective study, 120 courses of palliative radiotherapy for gastric cancer bleeding in 117 patients in 4 institutes in Japan were reviewed with approval of the ethical committee in each institute. The rate of achieving hemostasis was evaluated by 50% or more reduction of red blood cell transfusion before and after the start of radiotherapy, elevation of blood hemoglobin concentration in a period of 4 weeks from the start of radiotherapy or improvement of subjective or objective clinical symptoms in a period of 4 weeks from the start of radiotherapy. Predictive factors for overall survival and achieving hemostasis were investigated with the Cox hazards model.Results: The median overall survival period was 3.7 months. Multivariate analysis showed that absence of metastatic disease, higher biological effective dose, higher serum albumin level, lower blood urea nitrogen level and lower neutrophil-to-lymphocyte ratio (NLR) were associated with longer overall survival. Elevation of hemoglobin concentration in a period of 4 weeks from the start of radiotherapy (mean concentration: 8.2 g/dL vs. 8.9 g/dL, p=0.006) and decrease in the amount of red cell transfusion from a 4-week period before to a 4-week period after the start of radiotherapy (mean amount: 716 mL vs. 230 mL, p<0.0001) were observed. The overall rate of achievement of hemostasis was 59.6%. In multivariate analysis, higher biological effective dose was associated with achievement of hemostasis. Grade 2 or higher acute adverse effects related to radiotherapy were observed in 17.5% of cases in 120 treatment courses. Six cases (5.0%) had grade 3 or 4 adverse effects including gastric penetration in 1 patient and anorexia requiring total parental nutrition in 3 patients. No grade 5 adverse effects were observed.Conclusions: Palliative radiotherapy for gastric cancer bleeding seems to be an effective and safe treatment strategy. Higher treatment dose was associated with longer overall survival and a hemostatic effect. Some hematological parameters may predict overall survival, and they would be helpful for deciding the treatment strategy.
Multi-Conformational Luminescence and Phosphorescence of Few Phenazine 1,2,3-Triazole Molecules
Authors: Pinto Pablo B. , Cruz Kimberly C. T. da , Júnior Eufrânio Nunes da Silva , Cury Luiz Alberto
Published: January 2022

Dropcast films produced from blends solutions of phenazine 1,2,3-triazole molecules in very low concentrations in a 1,3-Bis (N-carbazolyl) benzene (mCP) matrix were investigated at room temperature. The mCP acts as an optically inert matrix, having no influence on the emission properties of the guest molecules. Its conductive properties also ensure that blend films, within a completely organic character, are formed as truly active layers. The fluorescent and phosphorescent emission properties of the phenazine molecules, depending on their conformational states, allowed relatively intense emissions in blue, green, red and also in white, without the need to mix different materials. Although the results of absorption of the blended films have shown no characteristics of the guest molecules, due to their relatively low concentrations, the excitation of them occurs directly by the incident laser beam. The steady-state spectroscopy for the monomer and dimer singlet fluorescence states of respective blue and green emissions of the films were investigated. The analysis of their temporal decays were done using a different approach based on the Exponentially Modified Gaussian (EMG) function. The phosphorescent emissions of the triplet steady-states, occurring in the orange or in the red wavelength regions, were observed to be correlated, respectively, to the formation of guest monomers or to the guest dimers singlet states.
NRF2 Participates in the Suppressive Tumor Immune Microenvironment of KRAS/KEAP1 Co-Mutant Non-Small Cell Lung Cancer by Inhibiting the STING Pathway
Authors: Sun Xiaodan , Zhao Peiyan , Li Hui , liu Yan , Cheng Ying
Published: January 2022

Background: KRAS/KEAP1 (KK) co-mutant lung adenocarcinoma (LUAD) exhibited poor response to immune checkpoint inhibitors (ICI) via shaping a suppressive tumor immune microenvironment, the mechanism remains to be elucidated. Methods: The mRNA and protein expression of target molecules were analyzed by qRT-PCR and Western blot, respectively. The subcellular location of NRF2 was observed by immunofluorescence staining, and nuclear and cytoplasm isolation. After exogenous over-expression and knockdown of NRF2 and the addition of a STING pathway inhibitor in tumor cells, the effects on the CD8+ T cell recruitment was detected using chemotaxis assay, and the secretion of chemokines CCL5 and CXCL10 was analyzed by ELISA. The potential NRF2 target BRCA1 was identified using bioinformatic approaches and verified by a dual luciferase reporter assay. Results: NRF2, the target of KEAP1, was overexpressed and activated in KK type cells. NRF2 effected as a negative regulator of CD8+ T cells recruitment by decreasing CCL5 and CXCL10 chemokines in KK type LUAD. Mechanistically, NRF2 promoted the transcription and expression of BRCA1 to repair DNA damage, resulting in STING pathway inactivation. Conclusion: The combination of NRF2 inhibitor or STING agonist with ICI may be a promising therapeutic approach for patients with KK type LUAD.
Using Threshold Analysis to Assess the Robustness of Public Health Intervention Recommendations from Network Meta-Analyses: Application to Poison Prevention in Households with Children Under Five
Authors: Wells Molly , Bujkiewicz Sylwia , Hubbard Stephanie J
Published: January 2022

BackgroundIn the appraisal of clinical interventions, complex evidence synthesis methods, such as network meta-analysis (NMA), are commonly used to investigate the effectiveness of multiple interventions in a single analysis. The results from a NMA can inform clinical guidelines directly or be used as inputs into a decision-analytic model assessing the cost-effectiveness of the interventions. However, there is hesitancy in using complex evidence synthesis methods when evaluating public health interventions. This is due to significant heterogeneity across studies investigating such interventions and concerns about their quality. Threshold analysis has been developed to help assess and quantify the robustness of recommendations made based on results obtained from NMAs to potential limitations of the data. Developed in the context of clinical guidelines, the method may prove useful also in the context of public health interventions. In this paper, we illustrate the use of the method in the study investigating the effectiveness of interventions aiming to increase the uptake of poison prevention behaviours in homes with children aged 0-5.MethodsRandom effects NMA was carried out to assess the effectiveness of several interventions for increasing the uptake of poison prevention behaviours, focusing on the safe storage of other household products outcome. Threshold analysis was then applied to the NMA to assess the robustness of the intervention recommendations made based on the NMA.Results15 studies assessing seven interventions were included in the NMA. The results of the NMA indicated that complex intervention, including Education, Free/low-cost equipment, Fitting equipment and Home safety inspection, was the most effective intervention at promoting poison prevention behaviours. However, the threshold analyses highlighted that this intervention recommendation was not robust.Conclusions In our case study, threshold analysis allowed us to demonstrate that the intervention recommendation for promoting poison prevention behaviours was not robust to changes in the evidence due to potential bias. Therefore, caution should be taken when considering such interventions in practice. We have illustrated the potential benefit of threshold analysis and, therefore, encourage the use of the method in practice as a sensitivity analysis for NMA of public health interventions.
Predictor Naïve Temporal Baseline Hazard of Recurrent Ischemic Stroke
Authors: Elhefnawy Marwa Elsaeed , Ghadzi Siti Maisharah Sheikh , Albitar Orwa , Tangiisuran Balamurugan , Zainal Hadzliana , Looi Irene , Sidek Norsima , Aziz Zariah Abdul , Harun Sabariah Noor
Published: January 2022

There are established correlation between risk factors and the recurrence of ischemic stroke (IS), however does the hazard of recurrent IS change although without the influence of established risk factors? This study aimed to quantify the hazard of recurrent IS at different time points after the index IS. This was a population cohort study extracted data of 7697 patients with a history of first IS attack registered with National Neurology Registry of Malaysia. A repeated time to recurrent IS model was developed using NONMEM version 7.5. Three baseline hazard models were fitted into the data. The best model was selected using maximum likelihood estimation, clinical plausibility and visual predictive checks. Three hundred and thirty-three (4.32%) patients developed at least one recurrent IS within the maximum 7.37 years follow-up. In the absence of significant risk factors, the hazard of recurrent IS was predicted to be 0.71 within the first month after the index IS and reduced to 0.022 between the first to third months after the index attack. The hazard of IS recurrence accelerated with the presence of typical risk factors such as hyperlipidaemia (HR, 2.64 [2.10-3.33]), hypertension (HR, 1.97 [1.43-2.72], and ischemic heart disease (HR, 2.21 [1.69-2.87]). In conclusion, the absence of significant risk factors, predicted hazard of recurrent IS was prominent in the first month after the index IS and was non-zero even three months after the index IS or later. Optimal secondary preventive treatment should incorporate the ‘nature risk’ IS recurrence.
Dexmedetomidine Induces Microglial Activation and Modulates Microglial M1/M2 Polarization in Attenuation of Chronic Morphine Tolerance in Cancer Pain
Authors: Bu Jianlong , Xiaohong Wu , Lin Deng , Chao Ma , Xiaoding Shi , ZHANG PINYI , Guonian Wang
Published: January 2022

The pro-inflammatory (M1) and anti-inflammatory (M2) status of microglial determines the outcome of neuroinflammation, which contributes to the pathogenesis of chronic morphine tolerance. Studies report that α2-adrenoceptor agonist dexmedetomidine exerts anti-inflammatory effects in inhibiting morphine tolerance in normal and neuropathic pain animals, which has not been studied in cancer pain. Therefore, we investigate the effect of intrathecal DEX on morphine tolerance in cancer pain, and whether dexmedetomidine functions via modulating microglial activation and M1/M2 polarization. 54 Wistar rats with intrathecal catheterization were treated by morphine for 10 days. Test groups received intrathecal α2-adrenoceptor agonist dexmedetomidine or antagonist MK-467. The mRNA levels of TLR4 and NF-κB were tested by RT-PCR. The protein levels of TLR4, NF-κB, Iba-1, iNOS, CD206 were quantifed using Western blotting, and IL-10 and TNF-α were examined by ELISA. Dexmedetomidine attenuates mechanical threshold and thermal latency, and increased the expression of TLR4 and NF-κB in morphine tolerance of cancer pain. Dexmedetomidine attenuates mechanical and thermal nociception in morphine tolerance in cancer pain rats. Intrathecal DEX pre-treatment significantly increased the protein levels of microglia maker Iba-1, M2 marker CD206 and anti-inflammatory factor IL-10, while had no evident influence on the pro-inflammatory factor TNF-α and M1 marker iNOS in morphine tolerance. Our findings suggest that intrathecal dexmedetomidine attenuates morphine tolerance in cancer pain via α2-adrenoceptor pathway. Furthermore, dexmedetomidine upregulates TLR4/NF-κB pathway and induces microglia activation in chronic morphine tolerance of cancer pain. The anti-inflammatory effect of dexmedetomidine might be exerted by inducing microglia M2 polarization and increasing anti-inflammatory factor IL-10.
Prevalence and Burden of Gastrointestinal Parasites in Stray Cattle of Kathmandu Valley
Authors: Thapa Bigyan , Parajuli Rajendra Prasad , Dhakal Pitambar
Published: January 2022

Gastrointestinal parasites (GIPs) are ubiquitous among cattle resulting severe infection. Prevalence of GIPs in stray street cattle may pose risk of dissemination of parasites of zoonotic importance. This study was conducted to determine the prevalence of GIPs in stray cattle of Kathmandu valley. Hundred (n=100) freshly voided dung samples were collected from eight places. The samples were processed using concentration method for microscopic examination, and modified McMaster technique for quantification of mean eggs/oocysts per gram of feces (EPG/OPG). Results revealed that 72% of the cattle were found positive for one or more species of GIPs and nine genera of GIPs were recorded (Eimeria, Ostertagia, Haemonchus, Trichostrongylus, Capillaria, Trichuris, Toxocara, Fasciola and Paramphistomum). The prevalence of parasitic infection was higher in male (73.68%) than in female (69.76%). The prevalence was found to be highest in adults (63.89%) followed by heifers (27.78%) and calves (8.33%). Approximately 76% of the cross breed and 65% local breed of cattle were positive for parasitic infection. The parasites differed both in prevalence and intensity, Eimeria sp. being the most prevalent (27%) with highest intensity (858.02 OPG ±63.46 SD). To our information, this is the first research of its kind in relation to stray cattle in Nepal. Our findings reveal that there is burden of helminth infections of zoonotic and socioeconomic importance in the straycattle. Therefore, it warrants regular inspection, relevant preventive measures and molecular detection of parasites.
The Clinical Impact of Neoadjuvant Endocrine Treatment On Luminal-Like Breast Cancers and Its Prognostic Significance: Results From a Single-institution Prospective Cohort Study
Authors: Martí Covadonga , Yébenes Laura , Oliver José María , Moreno Elisa , Frías Laura , Berjón Alberto , Loayza Adolfo , Meléndez Marcos , Roca María José , Córdoba Vicenta , Hardisson David , Rodríguez María Ángeles , Sánchez-Méndez José Ignacio
Published: January 2022

Purpose: Neoadjuvant endocrine treatment (NET) has become a useful tool for the downstaging of luminal-like breast cancers in postmenopausal patients. It enables us to increase breast conserving surgery (BCS) rates, and provides an opportunity for assessing in vivo NET effectiveness and studying any biological changes that may act as valid biomarkers. The purpose of this study was to evaluate the safety and effectiveness of NET, and to assess the role of Ki67 proliferation rate changes as an indicator of endocrine responsiveness.Methods: From 2016 to 2020, a single-institution cohort of patients treated with NET and further surgery was evaluated. In patients with Ki67≥10%, a second core biopsy was performed after four weeks. Information regarding histopathological and clinical changes was gathered.Results: A total of 115 estrogen receptor positive (ER+)/HER2 negative patients were included. The median treatment duration was 5.0 months (IQR: 2.0-6.0). Median maximum size in the surgical sample was 40% smaller than pretreatment size measured by ultrasound (p<.0001). Median pretreatment Ki67 expression was 20.0% (IQR: 12.0-30.0), and was reduced to 5.0% (IQR: 1.8-10.0) after four weeks, and to 2.0% (IQR: 1.0-8.0) in the surgical sample (p<.0001). BCS was performed on 98 patients (85.2%). No pathological complete responses were recorded. A larger Ki67 fold-change after four weeks was significantly related to a PEPI score of 0 (p<.002). No differences were observed between luminal A- and B-like tumors with regard to fold-change and PEPI score.Conclusions: In our cohort, NET has proven effective for tumor size and Ki67 downstaging. This results in a higher rate of conservative surgery, aids in therapeutic decision-making, provides prognostic information, and constitutes a safe and well-tolerated approach