bluuebunny/crossref_metadata_2025 huggingface dataset:
Working Memory Performance under a Negative Affect Is More Susceptible to Higher Cognitive Workloads with Different Neural Haemodynamic Correlates
Authors: Feng Ying Xing , Kiguchi Masashi , Ung Wei Chun , Dass Sarat Chandra , Mohd Hani Ahmad Fadzil , Tang Tong Boon , Ho Eric Tatt Wei
Published: July 2021

The effect of stress on task performance is complex, too much or too little stress negatively affects performance and there exists an optimal level of stress to drive optimal performance. Task difficulty and external affective factors are distinct stressors that impact cognitive performance. Neuroimaging studies showed that mood affects working memory performance and the correlates are changes in haemodynamic activity in the prefrontal cortex (PFC). We investigate the interactive effects of affective states and working memory load (WML) on working memory task performance and haemodynamic activity using functional near-infrared spectroscopy (fNIRS) neuroimaging on the PFC of healthy participants. We seek to understand if haemodynamic responses could tell apart workload-related stress from situational stress arising from external affective distraction. We found that the haemodynamic changes towards affective stressor- and workload-related stress were more dominant in the medial and lateral PFC, respectively. Our study reveals distinct affective state-dependent modulations of haemodynamic activity with increasing WML in n-back tasks, which correlate with decreasing performance. The influence of a negative effect on performance is greater at higher WML, and haemodynamic activity showed evident changes in temporal, and both spatial and strength of activation differently with WML.
Genetic Landscape of Male Breast Cancer
Authors: Campos Fernando Augusto Batista , Rouleau Etienne , Torrezan Giovana Tardin , Carraro Dirce Maria , Casali da Rocha José Claudio , Mantovani Higor Kassouf , da Silva Leonardo Roberto , Osório Cynthia Aparecida Bueno de Toledo , Moraes Sanches Solange , Caputo Sandrine M. , Santana dos Santos Elizabeth
Published: July 2021

Male breast cancer (MBC) is now considered molecularly different from female breast cancer (FBC). Evidence from studies indicates that common genetic and epigenetic features of FBC are not shared with those diagnosed in men. Genetic predisposition is likely to play a significant role in the tumorigenesis of this rare disease. Inherited germline variants in BRCA1 and BRCA2 account for around 2% and 10% of MBC cases, respectively, and the lifetime risk of breast cancer for men harboring BRCA1 and BRCA2 mutations is 1.2% and 6.8%. As for FBC, pathogenic mutations in other breast cancer genes have also been recently associated with an increased risk of MBC, such as PALB2 and CHEK2 mutations. However, while multigene germline panels have been extensively performed for BC female patients, the rarity of MBC has resulted in limited data to allow the understanding of the magnitude of risk and the contribution of recently identified moderate penetrance genes of FBC for MBC predisposition. This review gathers available data about the germline genetic landscape of men affected by breast cancer, estimated risk associated with these genetic variants, and current guidelines for clinical management.
Neurocognitive Outcomes in Pediatric Patients Following Brain Irradiation
Authors: Weusthof Katharina , Lüttich Peggy , Regnery Sebastian , König Laila , Bernhardt Denise , Witt Olaf , Herfarth Klaus , Unterberg Andreas , Jungk Christine , Farnia Benjamin , Combs Stephanie E. , Debus Jürgen , Rieken Stefan , Harrabi Semi , Adeberg Sebastian
Published: July 2021

Advanced radiation techniques can reduce the severity of neurocognitive sequelae in young brain tumor patients. In the present analysis, we sought to compare neurocognitive outcomes after proton irradiation with patients who underwent photon radiotherapy (RT) and surgery. Neurocognitive outcomes were evaluated in 103 pediatric brain tumor patients (proton RT n = 26, photon RT n = 30, surgery n = 47) before and after treatment. Comparison of neurocognitive outcomes following different treatment modalities were analyzed over four years after treatment completion. Longitudinal analyses included 42 months of follow-up after proton RT and 55 months after photon RT and surgery. Neurocognitive assessment included standardized tests examining seven domains. A comparison of neurocognitive outcomes after RT (proton and photon with >90% additional surgery) and surgery showed no significant differences in any neurocognitive domain. Neurocognitive functioning tests after proton RT failed to identify alterations compared to baseline testing. Long-term follow up over four years after photon RT showed a decrease in non-verbal intelligence (−9.6%; p = 0.01) and visuospatial construction (−14.9%; p = 0.02). After surgery, there was a decline in non-verbal intelligence (−10.7%; p = 0.01) and processing speed (14.9%; p = 0.002). Differences in neurocognitive outcomes between RT and surgical cohorts in direct intermodal comparison at long-term follow-up were not identified in our study, suggesting that modern radiation therapy does not affect cognition as much as in the past. There were no alterations in long-term neurocognitive abilities after proton RT, whereas decline of processing speed, non-verbal intelligence, and visuospatial abilities were observed after both photon RT and surgery. Domains dependent on intact white matter structures appear particularly vulnerable to brain tumor treatment irrespective of treatment approach.
Text Messaging in Cancer-Supportive Care: A Systematic Review
Authors: Wijeratne Don Thiwanka , Bowman Meghan , Sharpe Isobel , Srivastava Siddhartha , Jalink Matthew , Gyawali Bishal
Published: July 2021

The global cancer disease burden is substantial, resulting in increased economic and clinical strain on our healthcare systems. A proposed solution is text-based communication, which can be used for cancer-supportive care. We conducted a systematic review to synthesize and describe the use of text-based communications for cancer-supportive care. Our population of interest included adult patients with cancer. A total of 18 studies were included in the review: 9 RCTs and 9 non-randomized interventional/observational studies. Patients were largely satisfied with text-based communication during their cancer care. Compared to controls, results for other outcomes including symptoms and quality of life were largely mixed; however, no harms were observed. Furthermore, positive outcomes were seen for specific interventions, such as text message medication reminders. These findings should be considered with caution due to the considerable heterogeneity observed between studies regarding their design and reported outcomes and the high risk of bias associated with 6/18 studies. Overall, this review suggests that text-based communication may be a complementary tool for cancer-supportive care; however, more research is needed to examine the feasibility of implementation and use.
Cigarette Smoke Containing Acrolein Upregulates EGFR Signaling Contributing to Oral Tumorigenesis In Vitro and In Vivo
Authors: Tsou Han-Hsing , Tsai Hong-Chieh , Chu Chiao-Ting , Cheng Hsiao-Wei , Liu Chung-Ji , Lee Chien-Hung , Liu Tsung-Yun , Wang Hsiang-Tsui
Published: July 2021

Oral squamous cell carcinoma (OSCC) accounts for 80–90% of all intraoral malignant neoplasms. The single greatest risk factor for oral cancer is tobacco use, including cigarettes, cigars, chewing tobacco, and snuff. Aberrations of the epidermal growth factor receptor (EGFR) pathway features prominently in oral tumorigenesis and progression. It was shown that cigarette smoking (CS) is associated with worse prognosis in OSCC patients and overexpression of EGFR in tumor tissue. However, the mechanism by which cigarette smoking induced EGFR pathway activation remains to be fully elucidated. Acrolein, an IARC group 2A carcinogen, is a highly reactive aldehyde found in CS. Here we report that acrolein is capable of inducing tumorigenic transformation in normal human oral keratinocytes (NOK). The acrolein-transformed NOK cells showed EGFR copy number amplification, increased EGFR expression, and activation of downstream ERK and AKT signaling pathway. No p53 mutations were observed in acrolein-transformed NOK cells. Inhibiting EGFR pathway using an anti-EGFR antibody, cetuximab, inhibits tumor growth. Furthermore, by examining tissue sample from patients, we found an increased EGFR copy number was positively associated with acrolein-induced DNA damages in OSCC patients. Taken together, our results indicate that acrolein is important in tumorigenic transformation through amplification of EGFR and activating the downstream signaling pathway, contributing to oral carcinogenesis. This is the first study to provide molecular evidence showing that CS containing acrolein contributes to EGFR amplification in OSCC.
Biochemical Mechanisms Associating Alcohol Use Disorders with Cancers
Authors: Rodriguez Francisco D. , Coveñas Rafael
Published: July 2021

The World Health Organization identifies alcohol as a cause of several neoplasias of the oropharynx cavity, esophagus, gastrointestinal tract, larynx, liver, or female breast. We review ethanol’s nonoxidative and oxidative metabolism and one-carbon metabolism that encompasses both redox and transfer reactions that influence crucial cell proliferation machinery. Ethanol favors the uncontrolled production and action of free radicals, which interfere with the maintenance of essential cellular functions. We focus on the generation of protein, DNA, and lipid adducts that interfere with the cellular processes related to growth and differentiation. Ethanol’s effects on stem cells, which are responsible for building and repairing tissues, are reviewed. Cancer stem cells (CSCs) of different origins suffer disturbances related to the expression of cell surface markers, enzymes, and transcription factors after ethanol exposure with the consequent dysregulation of mechanisms related to cancer metastasis or resistance to treatments. Our analysis aims to underline and discuss potential targets that show more sensitivity to ethanol’s action and identify specific metabolic routes and metabolic realms that may be corrected to recover metabolic homeostasis after pharmacological intervention. Specifically, research should pay attention to re-establishing metabolic fluxes by fine-tuning the functioning of specific pathways related to one-carbon metabolism and antioxidant processes.
Multi-Layer Nanofibrous PCL Scaffold-Based Colon Cancer Cell Cultures to Mimic Hypoxic Tumor Microenvironment for Bioassay
Authors: Oh Eun-Taex , Kim Ha Gyeong , Choi Min-Ho , Lee Jae-Seon , Kim Sang Jeong , Kwak Jong-Young , Park Heon Joo
Published: July 2021

Three-dimensional (3D) cancer cell culture systems have been developed to aid the study of molecular mechanisms in cancer development, identify therapeutic targets, and test drug candidates. In this study, we developed a strategy for mimicking the hypoxic tumor microenvironment in a 3D cancer cell culture system using multi-layer, nanofibrous poly(ε-caprolactone) (PCL) scaffold (pNFS)-based cancer cell cultures. We found that human colon cancer cells infiltrated pNFS within 3 days and could be cultured three-dimensionally within the NFS. When incubated in four stacks of 30 µm-thick pNFS for 3 days, colon cancer cells in layer three showed partially reduced entry into the S phase, whereas those in layer four, located farthest from the media, showed a marked reduction in S-phase entry. As a consequence, cells in layer four exhibited hypoxia-induced disorganization of F-actin on day 3, and those in layers three and four showed an increase in the expression of the hypoxia-induced transcription factor HIF-1α and its target genes, Glut1, CA9, VEGF, and LDHA. Consistent with these results, doxorubicin- and ionizing radiation-induced cell death was reduced in colon cancer cells cultured in layers three and four. These results suggest that pNFS-based multi-layer colon cancer cell cultures mimic the hypoxic tumor microenvironment and are useful for bioassays.
Oxidation of Sulfamethoxazole by Rice Husk Biochar-Activated Persulfate
Authors: Avramiotis Efstathios , Frontistis Zacharias , Manariotis Ioannis D. , Vakros John , Mantzavinos Dionissios
Published: July 2021

In the present study, biochars from rice husk were synthesized via pyrolysis at 400, 550, 700 and 850 °C for 1 h under a limited O2 atmosphere, characterized with a various techniques of and used as catalysts to activate persulfate and to degrade sulfamethoxazole (SMX). After physicochemical characterization of biochars. SMX degradation tests were performed using different water matrices, persulfate biochar and SMX concentrations and different initial pH solutions. Also, spiked solutions with bicarbonate, chloride, calcium nitrate, humic acid or alcohols were tested. It was found that catalytic reactivity rises with the pyrolysis temperature. Biochar is crucial for the oxidation of SMX and it can be described with a pseudo first–order kinetic model. Real matrices hinder the oxidation process, in waste water the SMX removal is 41% in 90 min, comparable with the inhibition obtained with spiked with bicarbonates solution (52% removal within 90 min) while complete removal can be achieved in ultrapure water matrices. The presence of alcohol slightly inhibits degradation contrary to the addition of sodium azide which causes significant inhibition, this is an evidence that degradation either under electron transfer/singlet oxygen control or dominated by surface-bound radicals.
GSI Treatment Preserves Protein Synthesis in C2C12 Myotubes
Authors: Huot Joshua R. , Thompson Brian , McMullen Charlotte , Marino Joseph S. , Arthur Susan T.
Published: July 2021

It has been demonstrated that inhibiting Notch signaling through γ-secretase inhibitor (GSI) treatment increases myogenesis, AKT/mTOR signaling, and muscle protein synthesis (MPS) in C2C12 myotubes. The purpose of this study was to determine if GSI-mediated effects on myogenesis and MPS are dependent on AKT/mTOR signaling. C2C12 cells were assessed for indices of myotube formation, anabolic signaling, and MPS following GSI treatment in combination with rapamycin and API-1, inhibitors of mTOR and AKT, respectively. GSI treatment increased several indices of myotube fusion and MPS in C2C12 myotubes. GSI-mediated effects on myotube formation and fusion were completely negated by treatment with rapamycin and API-1. Meanwhile, GSI treatment was able to rescue MPS in C2C12 myotubes exposed to rapamycin or rapamycin combined with API-1. Examination of protein expression revealed that GSI treatment was able to rescue pGSK3β Ser9 despite AKT inhibition by API-1. These findings demonstrate that GSI treatment is able to rescue MPS independent of AKT/mTOR signaling, possibly via GSK3β modulation.
Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells
Authors: Ahmed Ebtehal , Saleh Tarek , Xu Meifeng
Published: July 2021

The functionalization of decellularized scaffolds is still challenging because of the recellularization-related limitations, including the finding of the most optimal kind of cell(s) and the best way to control their distribution within the scaffolds to generate native mimicking tissues. That is why researchers have been encouraged to study stem cells, in particular, mesenchymal stem cells (MSCs), as alternative cells to repopulate and functionalize the scaffolds properly. MSCs could be obtained from various sources and have therapeutic effects on a wide range of inflammatory/degenerative diseases. Therefore, in this mini-review, we will discuss the benefits using of MSCs for recellularization, the factors affecting their efficiency, and the drawbacks that may need to be overcome to generate bioengineered transplantable organs.
Consequences of SUR2[A478V] Mutation in Skeletal Muscle of Murine Model of Cantu Syndrome
Authors: Scala Rosa , Maqoud Fatima , Zizzo Nicola , Passantino Giuseppe , Mele Antonietta , Camerino Giulia Maria , McClenaghan Conor , Harter Theresa M. , Nichols Colin G. , Tricarico Domenico
Published: July 2021

(1) Background: Cantu syndrome (CS) arises from gain-of-function (GOF) mutations in the ABCC9 and KCNJ8 genes, which encode ATP-sensitive K+ (KATP) channel subunits SUR2 and Kir6.1, respectively. Most CS patients have mutations in SUR2, the major component of skeletal muscle KATP, but the consequences of SUR2 GOF in skeletal muscle are unknown. (2) Methods: We performed in vivo and ex vivo characterization of skeletal muscle in heterozygous SUR2[A478V] (SUR2wt/AV) and homozygous SUR2[A478V] (SUR2AV/AV) CS mice. (3) Results: In SUR2wt/AV and SUR2AV/AV mice, forelimb strength and diaphragm amplitude movement were reduced; muscle echodensity was enhanced. KATP channel currents recorded in Flexor digitorum brevis fibers showed reduced MgATP-sensitivity in SUR2wt/AV, dramatically so in SUR2AV/AV mice; IC50 for MgATP inhibition of KATP currents were 1.9 ± 0.5 × 10−5 M in SUR2wt/AV and 8.6 ± 0.4 × 10−6 M in WT mice and was not measurable in SUR2AV/AV. A slight rightward shift of sensitivity to inhibition by glibenclamide was detected in SUR2AV/AV mice. Histopathological and qPCR analysis revealed atrophy of soleus and tibialis anterior muscles and up-regulation of atrogin-1 and MuRF1 mRNA in CS mice. (4) Conclusions: SUR2[A478V] “knock-in” mutation in mice impairs KATP channel modulation by MgATP, markedly so in SUR2AV/AV, with atrophy and non-inflammatory edema in different skeletal muscle phenotypes.
Curcuma longa Mediated Synthesis of Copper Oxide, Nickel Oxide and Cu-Ni Bimetallic Hybrid Nanoparticles: Characterization and Evaluation for Antimicrobial, Anti-Parasitic and Cytotoxic Potentials
Authors: Faisal Shah , Al-Radadi Najlaa , Jan Hasnain , Abdullah  , Shah Sajjad , Shah Sumaira , Rizwan Muhammad , Afsheen Zobia , Hussain Zahid , Uddin Muhammad , Idrees Muhammad , Bibi Nadia
Published: July 2021

Nanoparticles have long been known and their biomedical potent activities have proven that these can provide an alternative to other drugs. In the current study, copper oxide, nickel oxide and copper/nickel hybrid NPs were biosynthesized by using Curcuma longa root extracts as a reducing and capping agent, followed by characterization via UV-spectroscopy, Fourier transformed infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermo galvanometric analysis (TGA), and band gap. FTIR spectroscopy shows the availability of various functional groups and biomolecules such as carbohydrate, protein, polysaccharides, etc. The EDX peak confirmed that the elemental nickel and copper were present in large quantity in the analyzed sample. Scanning electron micrographs showed that the synthesized CuO-NPs and NiO-NPs were polyhedral uniform and homogeneous in morphology, while the copper/nickel hybrid NPs were well dispersed, spherical in shape, and uniform in size. TEM micrographs of CuO-NPs had 27.72 nm, NiO had 23.13 nm and, for their hybrid, the size was 17.38 nm, which was confirmed respectively. The CuO and NiO NPs possessed spherical- to multi-headed shapes, while their hybrid showed a complete spherical shape, small size, and polydispersed NPs. The XRD spectra revealed that the average particle size for CuO, NiO, and hybrid were 29.7 nm, 28 nm and 27 nm, respectively. Maximum anti-diabetic inhibition of (52.35 ± 0.76: CuO-NPs, 68.1 ± 0.93: NiO-NPs and 74.23 ± 0.42: Cu + Ni hybrids) for α-amylase and (39.25 ± 0.18 CuO-NPs, 52.35 ± 1.32: NiO-NPs and 62.32 ± 0.48: Cu + Ni hybrids) for α-glucosidase were calculated, respectively, at 400 µg/mL. The maximum antioxidants capacity was observed as 65.1 ± 0.83 μgAAE/mg for Cu-Ni hybrids, 58.39 ± 0.62 μgAAE/mg for NiO-NPs, and 52.2 ± 0.31 μgAAE/mg for CuO-NPs, respectively, at 400 μg/mL. The highest antibacterial activity of biosynthesized NPs was observed against P. aeuroginosa (28 ± 1.22) and P. vulgaris (25 ± 1.73) for Cu + Ni hybrids, respectively. Furthermore, the antibiotics were coated with NPs, and activity was noted. Significant anti-leishmanial activity of 60.5 ± 0.53 and 68.4 ± 0.59 for Cu + Ni hybrids; 53.2 ± 0.48 and 61.2 ± 0.44 for NiO-NPs; 49.1 ± 0.39 and 56.2 ± 0.45 for CuO-NPs at 400 μg/mL were recorded for promastigote and amastigotes, respectively. The biosynthesized NPs also showed significant anti-cancerous potential against HepG2 cell lines. It was concluded from the study that NPs are potential agents to be used as an alternative to antimicrobial agents.
Removal of As(V) Based on Amino-Group Surface-Functionalized Porous Silicon Derived from Photovoltaic Silicon Cutting Powder
Authors: Fu Kaixin , Li Yi , Chen Xiumin , Ma Wenhui , Yang Ziheng , Wang Tong , Liu Jiasen , Ma Zhuang , Chen Ran , Chen Xiuhua , Li Shaoyuan
Published: July 2021

In this study, amino group surface-functionalized porous silicon adsorbent was successfully prepared for the first time using diamond wire saw silicon powder (DWSSP) as raw material through copper-assisted chemical etching (Cu-ACE) and organic functional group grafting. Amino-functionalized porous silicon adsorbent (TEPA-GTS-NPSi) can be used for removing As(V) from water. The properties and mechanism of the new adsorbent were characterized by infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (FE-SEM), Brunauer–Emmett–Teller analysis (BET), and thermogravimetric analysis (TGA). The concentration of metal ions in the solution was determined by inductively coupled plasma spectrometry. Meanwhile, the effects of initial pH, adsorption time, initial concentration and adsorbent dosage on the removal of As(V) in an aqueous solution were studied by intermittent adsorption experiments. The results showed that the adsorption equilibrium could be reached rapidly after 30 min soaking. Under the optimized pH of 7, the maximum adsorption capacity was 13.2 mg/g, and the minimum adsorption limit was 3 mg/L. The adsorbent shows good adsorption performance after five successive regenerated cycles. Based on the density functional theory (DFT) analysis results, the adsorption mechanism is attributed to hydrogen bond interaction between the NH2 group and As(V) ions.
Effects of Chromic Treatment on the Surface Properties of Polypropylene (PP) Wood Composites
Authors: Rejeb Marouan , Koubaa Ahmed , Elleuch Fayez , Godard François , Migneault Sébastien , Bouslimi Besma , Khlif Mohamed , Mrad Hatem
Published: July 2021

The moisture sensitivity of wood–polymer composites (WPCs) is mainly related to their hydrophilic wood components. Coatings are among the alternatives that improve the dimensional stability of these composites. However, the adhesion of most coatings to the WPC surface is generally poor. Thus, chemical and/or mechanical treatments should be applied to the WPC surface to improve the coating adhesion. Therefore, the main objective of this study was to improve the adhesion coating of polypropylene (PP) WPCs through a chromic treatment. PP was reinforced by three different pulp fibers (kraft, thermomechanical (TMP), and chemothermomechanical (CTMP)) at three fiber contents (50, 60, and 70% w/w). A chromic treatment was applied to the PP-based WPCs to activate the surface of the composites and alter their roughness parameters, creating a higher interfacial zone that improved the bonding of the epoxy coating to the surface of the PP composites. The chromic treatment increased the roughness of the surface. An increase in profile and surface parameters was observed after treatment. This treatment modified the chemical composition of the surface by creating polar carbon–oxygen groups and increasing the carbonyl and hydroxyl indexes.
Optimization Study of Fluffy Materials Flocking Drainage Pipes to Resist Blockage Based on MD Binding Energy
Authors: Liu Shiyang , Zhang Xuefu , Zhou Yuanfu , Gao Feng
Published: July 2021

Drainage pipe blockage resulting from crystals is one of the causes for cracking and leakage of tunnel lining. Therefore, effective prevention from drainage pipe blockage caused by crystals is crucial to ensure the safety and stability of lining structures during the operation of tunnel drainage system. Based on a large number of indoor model tests and numerical simulation analyses, binding energy between four materials and the calcium carbonate aqueous solution (“solid + liquid” system) and that between the four materials and the two typical growth crystals of calcium carbonate (“solid + solid” system) were studied. The research results indicated that: (1) The four materials all had an adsorption effect on the calcium carbonate aqueous solution system, and the PA6 had the greatest adsorption effect while the PP had the smallest adsorption effect; (2) There was spontaneous adsorption between the PVC or PA6 and the two typical growth crystals of calcium carbonate and no adsorption between the PP or SiC and the two typical growth crystals of calcium carbonate unless external energy was in place; (3) The PP and SiC can be used as the materials for drainage pipe flocking, but it shall be ensured that the fluffy material has a good geometrical property. The prevention technology for crystallization that causes drainage pipe blockage fills the gap in the research of drainage pipe blockage caused by crystals, which can reduce the maintenance cost for the operation of the tunnel drainage system and ensure safe and normal operation of the tunnel.
Reasonable Limiting of 7-Day Incidence per Hundred Thousand and Herd Immunization in Germany and Other Countries
Authors: Schlickeiser Reinhard , Kröger Martin
Published: July 2021

Based on hospital capacities, facts from past experience with the coronavirus disease 2019 (COVID-19) virus and the number of dark infections during the second wave (DII=2D2), a reasonable limiting value of 140/D2 for the 7-day incidence per 100,000 persons (MSDIHT) and a second wave herd immunization threshold fraction value of 0.26 in Germany were calculated. If the MSDIHT is held below this limiting value, the German hospital system can cope with the number of new seriously infected persons without any triage decisions. On the basis of the SIRV epidemics model, the classical threshold values for herd immunization were calculated for 18 countries. For these countries, the dates regarding when herd immunization against the second COVID-19 wave will be reached were estimated.
Molecular Characterization of the Native (Non-Linked) CD160–HVEM Protein Complex Revealed by Initial Crystallographic Analysis
Authors: Lenhartová Simona , Nemčovič Marek , Šebová Radka , Benko Mário , Zajonc Dirk M. , Nemčovičová Ivana
Published: July 2021

An increasing number of surface-exposed ligands and receptors acting on immune cells are being considered as a starting point in drug development applications. As they are dedicated to manipulating a wide range of immune responses, accurately predicting their molecular interactions will be necessary for the development of safe and effective therapeutics to enhance immune responses and vaccination. Here, we focused on the characterization of human CD160 and HVEM immune receptors, whose mutual engagement leads to bidirectional signaling (e.g., T cell inhibition, natural killer cell activation or mucosal immunity). In particular, our study reports on the molecule preparation, characterization and initial crystallographic analysis of the CD160–HVEM complex and both HVEM and CD160 in the absence of their binding partner. Despite the importance of the CD160–HVEM immune signaling and its therapeutic relevance, the structural and mechanistic basis underlying CD160–HVEM engagement has some controversial evidence. On one hand, there are studies reporting on the CD160 molecule in monomeric form that was produced by refolding from bacterial cells, or as a covalently linked single-chain complex with its ligand HVEM in insect cells. On the other hand, there are older reports providing evidence on the multimeric form of CD160 that acts directly on immune cells. In our study, the native non-linked CD160–HVEM complex was co-expressed in the baculovirus insect host, purified to homogeneity by anion-exchange chromatography to provide missing evidence of the trimeric form in solution. Its trimeric existence was also confirmed by the initial crystallographic analysis. The native CD160–HVEM complex crystallized in the orthorhombic space group with unit cell parameters that could accommodate one trimeric complex (3:3) in an asymmetric unit, thus providing ample space for the multimeric form. Crystals of the CD160–HVEM complex, CD160 trimer and HVEM monomer (reported in two space groups) diffracted to a minimum Bragg spacing of 2.8, 3.1 and 1.9/2.1 Å resolution, respectively. The obtained data will lead to elucidating the native structure of the complex.
Influence of LiCl and AgNO3 Doping on the Electrical Conductivity of PVA Flexible Electrolyte Polymer Film
Authors: Al Naim Abdullah F.
Published: July 2021

Recently, the electrical conductive electrolyte based on flexible polymeric films have been attracted much attentions, due to their applications in batteries, thermoelectrics, temperature sensors and others. In this regard, two polymeric electrolytes (PVA/LiCl) and (PVA/AgNO3) films have been engineered and the influence of the dopants and the annealing temperature on the structural, morphology and ac and dc conductivities is extensively studied. It was found that the films crystallinity has the order PVA/AgNO3 (49.44%) > PVA (38.64%) > PVA/LiCl (26.82%). Additionally, the dc conductivity of the films is increased with embedding the dopants into the PVA as the order PVA/AgNO3 (13.7 × 10−4 S/cm) > PVA/LiCl (1.63 × 10−5 S/cm) > PVA (1.71 × 10−6 S/cm) at 110 °C. It is also found that there is a sharp increase for σac as the frequency increases up to 107 Hz and also as the temperature increases to 110 °C. However, the order of increasing the σac is PVA/LiCl (155 × 10−3 S/cm) > PVA/AgNO3 (2.5 × 10−5 S/cm) > PVA (2 × 10−6 S/cm) at f = 107 Hz and 110 °C. The values of exponent are 0.870, 0.405 and 0.750 for PVA, PVA/AgNO3 and PVA/LiCl, respectively, and it is increased as the temperature increases for PVA and PVA/LiCl, but it is decreased for PVA/AgNO3. The activation energies Ea are 0.84, 0.51 and 0.62 eV for PVA, PVA/AgNO3 and PVA/LiCl, respectively. Moreover, the values of activation energy for charge carrier migration Em are 0.60, 0.34 and 0.4 eV for PVA, PVA/AgNO3 and PVA/LiCl, respectively. By using a simple approximation, the carrier concentration, carrier mobility and carrier diffusivity are calculated, and their values are increased as the temperature increases for all samples, but they are higher for PVA/LiCl than that of PVA/AgNO3. These results are discussed in terms of some obtained parameters such as hopping frequency, free volume and chain mobility. Interestingly, the conduction mechanism was found to be the electronic charge hopping for PVA and PVA/LiCl films, however it was found to be the ionic charge diffusion (n < 0.5) for PVA/AgNO3 film. It has been predicted that these electrolytic films have a prospective applications in batteries design, temperature sensors, electronic and wearable apparatuses at an affordable cost.
Characterizing the Influence of Domestic Cats on Birds with Wildlife Rehabilitation Center Data
Authors: Demezas K. Grace , Robinson W. Douglas
Published: July 2021

Depredation of birds by domestic cats is hypothesized to be one of many significant sources of mortality leading to global bird declines. Direct observations are relatively rarely documented compared with large numbers of birds hypothesized to be killed or wounded by cats. We analyzed data from two wildlife rehabilitation centers located in Salem and Grants Pass, Oregon USA, to understand which species were most likely to interact with a cat, and the species traits associated with cat interactions and habitats (urban vs. rural) of rescued birds. Interaction with a cat was the second-most commonly reported cause of admission, representing 12.3% of 6345 admissions. Half to two-thirds of birds were rescued from cats in urban settings and were usually species foraging on or near the ground. Most species were admitted to rehabilitation centers in direct proportion to their regional abundance. An exception was the absence of common species weighing less than 70 g, which we conclude is an effect of sampling bias. We conclude that cats most often interact with regionally common near-ground-dwelling bird species in both urban and rural habitats. Wildlife rehabilitation centers can provide valuable sources of data for cat-bird interactions but potential sources of uncertainty and bias in their data need to be considered carefully.
First Insights into the Microbiology of Three Antarctic Briny Systems of the Northern Victoria Land
Authors: Papale Maria , Rizzo Carmen , Caruso Gabriella , La Ferla Rosabruna , Maimone Giovanna , Lo Giudice Angelina , Azzaro Maurizio , Guglielmin Mauro
Published: July 2021

Different polar environments (lakes and glaciers), also in Antarctica, encapsulate brine pools characterized by a unique combination of extreme conditions, mainly in terms of high salinity and low temperature. Since 2014, we have been focusing our attention on the microbiology of brine pockets from three lakes in the Northern Victoria Land (NVL), lying in the Tarn Flat (TF) and Boulder Clay (BC) areas. The microbial communities have been analyzed for community structure by next generation sequencing, extracellular enzyme activities, metabolic potentials, and microbial abundances. In this study, we aim at reconsidering all available data to analyze the influence exerted by environmental parameters on the community composition and activities. Additionally, the prediction of metabolic functions was attempted by the phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt2) tool, highlighting that prokaryotic communities were presumably involved in methane metabolism, aromatic compound biodegradation, and organic compound (proteins, polysaccharides, and phosphates) decomposition. The analyzed cryoenvironments were different in terms of prokaryotic diversity, abundance, and retrieved metabolic pathways. By the analysis of DNA sequences, common operational taxonomic units ranged from 2.2% to 22.0%. The bacterial community was dominated by Bacteroidetes. In both BC and TF brines, sequences of the most thermally tolerant and methanogenic Archaea were detected, some of them related to hyperthermophiles.
Using NLP for Fact Checking: A Survey
Authors: Lazarski Eric , Al-Khassaweneh Mahmood , Howard Cynthia
Published: July 2021

In recent years, disinformation and “fake news” have been spreading throughout the internet at rates never seen before. This has created the need for fact-checking organizations, groups that seek out claims and comment on their veracity, to spawn worldwide to stem the tide of misinformation. However, even with the many human-powered fact-checking organizations that are currently in operation, disinformation continues to run rampant throughout the Web, and the existing organizations are unable to keep up. This paper discusses in detail recent advances in computer science to use natural language processing to automate fact checking. It follows the entire process of automated fact checking using natural language processing, from detecting claims to fact checking to outputting results. In summary, automated fact checking works well in some cases, though generalized fact checking still needs improvement prior to widespread use.
Laboratory Diagnosis of SARS-CoV-2 Pneumonia
Authors: Gitman Melissa R. , Shaban Maryia V. , Paniz-Mondolfi Alberto E. , Sordillo Emilia M.
Published: July 2021

The emergence and rapid proliferation of Coronavirus Disease-2019, throughout the past year, has put an unprecedented strain on the global schema of health infrastructure and health economy. The time-sensitive agenda of identifying the virus in humans and delivering a vaccine to the public constituted an effort to flatten the statistical curve of viral spread as it grew exponentially. At the forefront of this effort was an exigency of developing rapid and accurate diagnostic strategies. These have emerged in various forms over the past year—each with strengths and weaknesses. To date, they fall into three categories: (1) those isolating and replicating viral RNA in patient samples from the respiratory tract (Nucleic Acid Amplification Tests; NAATs), (2) those detecting the presence of viral proteins (Rapid Antigen Tests; RATs) and serology-based exams identifying antibodies to the virus in whole blood and serum. The latter vary in their detection of immunoglobulins of known prevalence in early-stage and late-stage infection. With this review, we delineate the categories of testing measures developed to date, analyze the efficacy of collecting patient specimens from diverse regions of the respiratory tract, and present the up and coming technologies which have made pathogen identification easier and more accessible to the public.
Course of Early Neurologic Symptom Severity after Endovascular Treatment of Anterior Circulation Large Vessel Occlusion Stroke: Association with Baseline Multiparametric CT Imaging and Clinical Parameters
Authors: Fabritius Matthias Philipp , Wölfer Teresa A. , Herzberg Moriz , Tiedt Steffen , Puhr-Westerheide Daniel , Grosu Sergio , Maurus Stefan , Geyer Thomas , Curta Adrian , Kellert Lars , Küpper Clemens , Liebig Thomas , Ricke Jens , Dimitriadis Konstantinos , Kunz Wolfgang G. , Zimmermann Hanna , Reidler Paul
Published: July 2021

Background: Neurologic symptom severity and deterioration at 24 hours (h) predict long-term outcomes in patients with acute large vessel occlusion (LVO) stroke of the anterior circulation. We aimed to examine the association of baseline multiparametric CT imaging and clinical factors with the course of neurologic symptom severity in the first 24 h after endovascular treatment (EVT). Methods: Patients with LVO stroke of the anterior circulation were selected from a prospectively acquired consecutive cohort of patients who underwent multiparametric CT, including non-contrast CT, CT angiography and CT perfusion before EVT. The symptom severity was assessed on admission and after 24 h using the 42-point National Institutes of Health Stroke Scale (NIHSS). Clinical and imaging data were compared between patients with and without early neurological deterioration (END). END was defined as an increase in ≥4 points, and a significant clinical improvement as a decrease in ≥4 points, compared to NIHSS on admission. Multivariate regression analyses were used to determine independent associations of imaging and clinical parameters with NIHSS score increase or decrease in the first 24 h. Results: A total of 211 patients were included, of whom 38 (18.0%) had an END. END was significantly associated with occlusion of the internal carotid artery (odds ratio (OR), 4.25; 95% CI, 1.90–9.47) and the carotid T (OR, 6.34; 95% CI, 2.56–15.71), clot burden score (OR, 0.79; 95% CI, 0.68–0.92) and total ischemic volume (OR, 1.01; 95% CI, 1.00–1.01). In a comprehensive multivariate analysis model including periprocedural parameters and complications after EVT, carotid T occlusion remained independently associated with END, next to reperfusion status and intracranial hemorrhage. Favorable reperfusion status and small ischemic core volume were associated with clinical improvement after 24 h. Conclusions: The use of imaging parameters as a surrogate for early NIHSS progression in an acute LVO stroke after EVT reached limited performance with only carotid T occlusion as an independent predictor of END. Reperfusion status and early complications in terms of intracranial hemorrhage are critical factors that influence patient outcome in the acute stroke phase after EVT.
Supervised Domain Adaptation for Automated Semantic Segmentation of the Atrial Cavity
Authors: Saiz-Vivó Marta , Colomer Adrián , Fonfría Carles , Martí-Bonmatí Luis , Naranjo Valery
Published: July 2021

Atrial fibrillation (AF) is the most common cardiac arrhythmia. At present, cardiac ablation is the main treatment procedure for AF. To guide and plan this procedure, it is essential for clinicians to obtain patient-specific 3D geometrical models of the atria. For this, there is an interest in automatic image segmentation algorithms, such as deep learning (DL) methods, as opposed to manual segmentation, an error-prone and time-consuming method. However, to optimize DL algorithms, many annotated examples are required, increasing acquisition costs. The aim of this work is to develop automatic and high-performance computational models for left and right atrium (LA and RA) segmentation from a few labelled MRI volumetric images with a 3D Dual U-Net algorithm. For this, a supervised domain adaptation (SDA) method is introduced to infer knowledge from late gadolinium enhanced (LGE) MRI volumetric training samples (80 LA annotated samples) to a network trained with balanced steady-state free precession (bSSFP) MR images of limited number of annotations (19 RA and LA annotated samples). The resulting knowledge-transferred model SDA outperformed the same network trained from scratch in both RA (Dice equals 0.9160) and LA (Dice equals 0.8813) segmentation tasks.
3D Underwater Uncooperative Target Tracking for a Time-Varying Non-Gaussian Environment by Distributed Passive Underwater Buoys
Authors: Hou Xianghao , Zhou Jianbo , Yang Yixin , Yang Long , Qiao Gang
Published: July 2021

Accurate 3D passive tracking of an underwater uncooperative target is of great significance to make use of the sea resources as well as to ensure the safety of our maritime areas. In this paper, a 3D passive underwater uncooperative target tracking problem for a time-varying non-Gaussian environment is studied. Aiming to overcome the low observability drawback inherent in the passive target tracking problem, a distributed passive underwater buoys observing system is considered and the optimal topology of the distributed measurement system is designed based on the nonlinear system observability analysis theory and the Cramer–Rao lower bound (CRLB) analysis method. Then, considering the unknown underwater environment will lead to time-varying non-Gaussian disturbances for both the target’s dynamics and the measurements, the robust optimal nonlinear estimator, namely the adaptive particle filter (APF), is proposed. Based on the Bayesian posterior probability and Monte Carlo techniques, the proposed algorithm utilizes the real-time optimal estimation technique to calculate the complex noise online and tackle the underwater uncooperative target tracking problem. Finally, the proposed algorithm is tested by simulated data and comprehensive comparisons along with detailed discussions that are made to demonstrate the effectiveness of the proposed APF.
Coreset Clustering on Small Quantum Computers
Authors: Tomesh Teague , Gokhale Pranav , Anschuetz Eric R. , Chong Frederic T.
Published: July 2021

Many quantum algorithms for machine learning require access to classical data in superposition. However, for many natural data sets and algorithms, the overhead required to load the data set in superposition can erase any potential quantum speedup over classical algorithms. Recent work by Harrow introduces a new paradigm in hybrid quantum-classical computing to address this issue, relying on coresets to minimize the data loading overhead of quantum algorithms. We investigated using this paradigm to perform k-means clustering on near-term quantum computers, by casting it as a QAOA optimization instance over a small coreset. We used numerical simulations to compare the performance of this approach to classical k-means clustering. We were able to find data sets with which coresets work well relative to random sampling and where QAOA could potentially outperform standard k-means on a coreset. However, finding data sets where both coresets and QAOA work well—which is necessary for a quantum advantage over k-means on the entire data set—appears to be challenging.
Optimal Computation of Network Indicators for Electricity Market Bidding Zones Configuration Considering Explicit N-1 Security Constraints
Authors: Bovo Cristian , Ilea Valentin , Carlini Enrico Maria , Caprabianca Mauro , Quaglia Federico , Luzi Luca , Nuzzo Giuseppina
Published: July 2021

In this paper an optimization problem designed to calculate electric grid specific indicators to be used within model-based methodologies for the definition of alternative electricity market bidding zone configurations is designed. The approach integrates within the framework of a bidding zone review process aligned to the specifications of the Commission Regulation (EU) 2015/1222 (CACM) and Regulation (EU) 2019/943 of the European Parliament and of the Council (CEP). The calculated solution of the optimization provides locational marginal prices and allows to determine, outside the optimization problem, the power transfer distribution factors for critical elements. Both indicators can be used as inputs by specially designed clustering algorithms to identify model-based electricity market bidding zone configurations, as alternative to the current experience-based configurations. The novelty of the optimization problem studied in this paper consists in integrating the N-1 security criteria for transmission network operation in an explicit manner, rather than in a simplified and inaccurate manner, as encountered in the literature. The optimization problem is evaluated on a set of historical and significant operating scenarios of the Italian transmission network, carefully selected by the Italian transmission system operator. The results show the optimization problem capability to produce insightful results for supporting a bidding zone review process and its advantages with respect to simplified methodologies encountered in the literature.
Rightsizing the Design of a Hybrid Microgrid
Authors: Reich Daniel , Oriti Giovanna
Published: July 2021

Selecting the sizes of distributed energy resources is a central planning element when designing a microgrid. Decision makers may consider several important factors, including, but not limited to, capacity, cost, reliability and sustainability. We introduce a method for rightsizing capacity that presents a range of potential microgrid design solutions, allowing decision makers to weigh their upsides and downsides based on a variety of measurable factors. We decouple component-specific modeling assumptions, energy management system logic and objective measurements from our simulation-based nested binary search method for rightsizing to meet power loads. In doing so, we develop a flexible, customizable and extensible approach to microgrid design planning. Aspects which have traditionally been incorporated directly in optimization-centric frameworks, such as resilience and reliability, can be treated as complementary analyses in our decoupled approach. This enables decision makers to gain exposure to a wide range of relevant information and actively participate in the microgrid design assessment process.