bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence and Risk Factors of Sub-clinical Mastitis in Lactating Dairy Cows with Special Emphasis on Antibiogram of the Causative Bacteria in Bangladesh
Authors: Sohidullah M. , Hossain Md. Jannat , Alam Muhammad Ashiqul , Rahman Nahid , Salauddin Md. , Matubber Bidyut
Published: June 2023

Background: Mastitis is one of the most devastating conditions for the dairy farms because of its alarming impact on production. Khulna is one of the dairy intensive regions of Bangladesh but comprehensive epidemiological studies regarding prevalence and risk factors of sub-clinical mastitis with antibiogram of the causative bacteria are scant. Therefore, an epidemiological study was conducted in Khulna district to investigate the prevalence and risk factor of sub-clinical mastitis in lactating dairy cows with antibiogram of the causative bacteria. Methods: Five dairy farms were selected and a total of 400 quarter milk samples of 100 dairy cows were subjected to California Mastitis Test to detect sub-clinical mastitis. Antibiogram study was performed to determine sensitivity and resistant pattern of the isolated bacteria. Results: An overall cow level prevalence of sub-clinical mastitis were recorded as 28%. Risk factors like type of breed, body condition score, milk yield, grass feeding, udder washing before milking, drying of the udder after washing, production system, dry cow therapy, stimuli before milking and milking techniques were statistically significant for the occurrence of sub-clinical mastitis. Antibiogram study revealed that most of the isolated 33.03% E. coli and 16.96% Staphylococcus sp. were sensitive to amoxicillin, gentamicin and trimethoprim/sulphamethoxazole.
Molecular and Immunopathological Investigations of Marek’s Disease in HVT Vaccinated Chicken Flocks
Authors: Pandey Shailja , Lather Deepika , Kumar Aman , Nehra Vikas , Jangir Babu Lal , Narang Gulshan
Published: June 2023

Background: The present investigation was carried out on poultry carcasses brought to the Department of Veterinary Pathology, LUVAS, Hisar from Herpesvirus of turkeys (HVT) vaccinated chicken flocks and suspected for viral neoplastic conditions on the basis of necropsy. Conventional and molecular techniques for the confirmation of the Marek’s disease (MD) were studied. For differential diagnosis from other oncogenic viral infections immunohistochemistry as an important tool was investigated. Methods: The conventional diagnosis of MD) was done on basis of clinical signs, gross lesions and histopathology of proliferate lesions; as lymphomatous lesions consist of pleomorphic lymphocytes. For confirmatory diagnosis molecular techniques were employed to detect MD virus specific Meq gene by Taqman probe real time PCR and nested PCR. For differential diagnosis of mixed oncogenic viral infections, immunohistochemistry (IHC) using B and T cell differentiation markers (CD79 alpha and CD3) was employed. Result: Specific Meq gene by Taqman probe real time PCR was positive in 92.5% suspected cases; while nested PCR technique gave positivity in 55% cases. The immunohistochemical reactivity for CD3 gave positive reactivity in the cytoplasm and cell membrane and negative reaction for CD79 alpha in MD positive cases, while in cases with mixed infections of MDV and other viral neoplastic conditions, cells showed positive reactivity to both CD3 and CD79 alpha in neoplastic proliferates.
Efficacy of Chinese and Western Medicine on Endometritis in Dairy Cows: A Systematic Review and Meta-analysis
Authors: Chen Peirong , Liu Jinni , Ma Bingjie , Li Zhili , Qin Qingming , Wu Haigang
Published: June 2023

Endometritis is a frequent postpartum disease of dairy cows and inflicts considerable distress on the animals and costs to the dairy industry. This study evaluated the clinical efficacy of Chinese and Western Medicine combination treatments for dairy cow endometritis to provide a reference for the prevention and treatment of these infections. This analysis provides new information for veterinary medical research. We used the keywords ‘Chinese and Western Medicine, Traditional and Western Medicine, Chinese herbs, Traditional Chinese Medicine Traditional Medicine, Chinese, Zhong Yi Xue, Chinese Traditional Medicine, Chinese Medicine, Traditional, Endometritis, Metritis and Cow to assess relevant literature sources collected in CNKI, Wanfang, Vepsa, China Patent Information Network, PubMed, Web of Science and other domestic and foreign databases. We utilized Cochrane system instructions to perform a meta-analysis using RevMan 5. 3 and Stata 16.0 software. We identified 13 randomized clinical trials (RCT) involving 847 dairy cows including cows 435 treated with a combination of Chinese and Western Medicine and 412 cows treated with the Western Medicine approach. The effectiveness for the combination therapy was better than that of the control (antibiotic only) where the relative risk was 1.19 with 95% CI (1.12, 1.26) and Z =5.96, P less than 0.01. We found no significant heterogeneity between these studies (I2=0 less than 50%, P =0.50 less than 0.1) and publication bias was absent as investigated through funnel plots and the Egger regression model (P=0.197 greater than 0.05). These results indicated that although the Chinese herbal preparations differed between studies (n=13), when coupled with Western antibiotic treatments the animals recovered faster than did the control Western antibiotic treatments. These randomized control studies indicated that the efficacy of Chinese and Western Medicine on endometritis in dairy cows is greater than that of Western Medicine treatments.
Screening Smoke Tree (Cotinus coggygria Scop.) on Osmotic Stress using Polyethylene Glycol 6000 in vitro
Authors: Zholobova O.O. , Mogilevskaya I.V. , Melnik S.V.
Published: June 2023

Background: Droughts during the growing season are becoming increasingly common due to climate change and global warming. The study’s goal study was to assess the adaptation mechanisms of C. coggygria Scop. under simulated water deficit conditions using indicators characterized changes in the leaf stomatal apparatus and photosynthesizing system. Methods: The influence of osmotic polyethylene glycol 6000 on the research object C. coggygria regenerants was studied in vitro. Such indicators as morphological changes, pigment content, stomatal density and size and stomatal slit size were quantified. Result: Due to the influence of osmotic stress, there was a pigment content increase in the leaf plate on osmotic media. Stomatal density raised by 27.0-29.8% in explant cells cultivated on PEG 4.0-6.0%. Under the osmotic influence, there was a drop in the stomatal cells’ area and shape and the ratio of stomatal length to width in the regenerant leaves decreased from 1.5 to 1.0-1.1. These results indicate the adaptation of C. coggygria regenerants to stress caused by an artificial drought in vitro. The changes’ identification in the stomatal apparatus and the C. coggygria pigment ratio will accelerate the selective screening of drought-resistant plants for use in protective forestry.Background: Droughts during the growing season are becoming increasingly common due to climate change and global warming. The study’s goal study was to assess the adaptation mechanisms of C. coggygria Scop. under simulated water deficit conditions using indicators characterized changes in the leaf stomatal apparatus and photosynthesizing system.
Phantom Limb Pain: Current Concepts and Treatment Strategies
Authors: Nieto-Salazar María Alejandra
Published: June 2023

Phantom limb syndrome (PLS) is described as the perception of feelings originating from a limb that has been amputated or is otherwise absent [1]. Despite this physical absence, patients may experience various sensory and motor sensations as if the limb were still present. Phantom limb symptoms encompass a range of sensations, including the missing limb's presence, movements such as wiggling toes or opening and closing the hand, pressure, tingling, itching, temperature changes, and pain [1-3]. Phantom limb pain (PLP) is commonly encountered in these patients, and it can vary in intensity, duration, and quality, ranging from throbbing or stabbing sensations to burning or cramping feelings. There is a high statistical heterogeneity across prevalence studies due to the different time windows assessed [4-10]. However, it is estimated that approximately 60-80% of individuals who have undergone limb amputation may experience phantom limb sensations, and around 50-85% of those individuals may also experience phantom limb pain [11,12]. Several factors have been identified as potential risk factors for developing this type of pain, including the level of amputation, pre-existing pain, post-amputation pain, chronic pain history, and psychological factors (i.e., anxiety, depression, post-traumatic stress disorder) [12,13].
Kant’s views on preformation and epigenesis
Authors: Goy Ina
Published: June 2023

How does Kant repsond to the early modern preformation-epigenesis controversy? In part 1 of the paper, I will introduce the historical context: I provide an overview of important systematic characteristics of ovistic and animalculist preformationist (1.1) and mechanical and vitalistic epigenetic (1.2) early modern accounts of reproduction and heredity. In part 2 of the paper, I will introduce the scholarly debate (2.1) about Kant’s reception of the early modern controversy: while no one considers Kant a radical defender of preformation, some scholars consider him a more or less radical defender of epigenesis. A greater number of scholars read Kant’s position as a combination of preformationist and epigenetic elements. Others ignore or even deny any influence of the preformation-epigenesis controversy on Kant. Based on an analysis of preformationist (2.2) and epigenetic elements (2.3) in Kant’s relevant writings, I will support scholars (2.4) who claim that Kant’s position contains both, preformationist and epigenetic elements, but will do so on a far more comprehensive analysis of criteria. I will also go beyond existing discussions deciding whether Kant’s account was closer to ovistic or animalculist variants of preformation, and mechanical or vitalistic variants of epigenesis.
Development of Islamic Sharia-Based Curriculum in Islamic Universities in Aceh: A Study on the Implementation of Aceh Qanun Number 9 of 2015
Authors: Sulaiman Sulaiman
Published: June 2023

This study aims to examine the implementation of an Islamic Sharia-Based Curriculum (KBSI) at Islamic Colleges (PTKI) in Aceh. This qualitative study used the purposive sampling method to select the participants, including five college leaders and 50 lecturers from UIN Ar-Raniry, IAIN Lhokseumawe, University of Muhammadiyah Aceh, STIS Al-Hilal Sigli, and STAI Teungku Chik Pante Kulu. The data were collected through interviews, observation, and documentation. The research shows that the strategy for developing an Islamic Sharia-based Curriculum (KBSI) at PTKI in Aceh is conducted by an integrated model, separated subject curriculum and correlated curriculum. The policy for developing an Islamic sharia-based curriculum at PTKI in Aceh refers to philosophical, sociological, historical, and juridical foundations in the form of Aceh qanuns. It further finds that the development of an Islamic sharia-based curriculum at PTKI in Aceh is an effort to guide the young Acehnese generations who are sharia-compliant and tolerant using special autonomy opportunities. Meanwhile, the difficulties in developing this curriculum are due to Aceh's internal and external PTKI factors. The obstacles are due to internal factors where PTKI leaders do not respond to the opportunities for KBSI reconstruction. On the other hand, obstacles also deal with external factors where collaboration between PTKI and the Aceh government in the context of KBSI reconstruction is less optimal.
Preserving Child's Faith in Malaysian Law: A Maqasidic Approach
Authors: Noor Zanariah Binti , Lee Nazirah
Published: June 2023

Preserving faith is the most significant goal for Muslims. However, fulfilling these objectives in the best possible way according to the principle of maqāṣid al-sharī’ah(the objective of Islamic law) within the framework of the dual system of Malaysian law is challenging. Preserving faith within maqāṣid al-sharī’ah discussion provides enormous opportunities for Muslims to nurture and protect their Islamic faith while regulating the laws that prohibit any harmful elements that could jeopardize it. The Federal Constitution of Malaysia guarantees everyone's freedom of religion. However, freedom of religion provided under Malaysian law has restrictions due to the uniqueness of Malaysian history. This article critically discusses the maqasidic-approach adopted to preserve Muslim child's faith. This article examines the government's effort to maintain Muslim child's faith concerning the laws and policies of education and Malaysian law on issues linked to the conversion of Muslims to other faiths and vice versa. The court decision on child's religion cases indicates a distinct feature of Muslim and non-Muslims' right to freedom of religion under Malaysian law. There are prospects for utilizing the maqāṣid al-sharī’ah framework effectively to promote the preservation of a child's faith in the context of Malaysian law.
Echoing Syari'ah in City Spaces: The Framing Process and Political Mobilization of the Islamic Movement in North Sumatra
Authors: Riza Faisal
Published: June 2023

This article discusses the Islamic political movement in fighting for sharia as an ethic of life in urban areas in Indonesia. In a more detailed manner, this article explores the idea of sharia as the main ingredient in the framing process and explores the movement's strategy to resonate with this frame, which then opens up opportunities for the formation of a wave of support for serial movement actions in Medan, North Sumatra. With the framing model in social movement studies, the data was collected from observations and interviews with several movement actors, and several supporting documents. This study found that the idea of this movements such as “Negara Kesatuan Republik Indonesia Bersyari’ah” (Indonesia Nation State based on Sharia) which is close to the social psychology of the masses met with the right events to generate great support for the movement. The ability of the elites to concoct the movement ideas, and the management of social media to spread such framing, made a mass wave of supporters strengthen. The struggle for sharia in the public space played by the movement here basically does not have a clear political agenda unless, temporarily, it is used only in electoral momentum, resonating as an expressive channel of the defeat against other groups in controlling the city's political economy space. Here, I argue that the study of ideology in social movements requires an expansion of dimensions that can explain more fully why a movement immediately grows or shrinks briefly or lasts a long time.
A litigiosidade como fenômeno complexo: quanto mais se empurra, mais o sistema empurra de volta
Authors: Schilling Ferraz Taís
Published: June 2023

O artigo tem por objetivos investigar o fenômeno da litigiosidade e avaliar se está sendo retroalimentado e intensificado pelo modelo linear pelo qual o Judiciário dá tratamento aos conflitos. A metodologia utilizada é a pesquisa bibliográfica, a partir de revisão de literatura e coleta de dados secundários. O trabalho inicia com a avaliação, em série histórica, dos índices de judicialização e de recorribilidade, colhidos dos relatórios produzidos pelo Conselho Nacional de Justiça e do Supremo Tribunal Federal. Na sequência, à luz da pesquisa bibliográfica, confrontam-se os dados sobre litigiosidade com os pressupostos teórico-metodológicos do pensamento sistêmico, para identificar possíveis efeitos tautológicos e amplificadores sobre a litigiosidade, decorrentes da maneira reativa pela qual o Judiciário opera diante de novos casos e recursos. Ao final, o artigo propõe a intensificação no uso de estratégias específicas, com maior potencial de alavancagem na prevenção e no tratamento do fenômeno.
Medical nose reshaping: current evidence behind techniques
Authors: Diaspro Alberto , Redaelli Alessio , Saban Yves , Sulamanidze Constantin , Pascali Michele , Bertossi Dario
Published: June 2023

The nose, centrally located on the face, plays a crucial role in facial aesthetics. Any imbalance in its length, width, tip position, or a gross deformity, such as dorsal hump and deviation, can significantly affect the overall facial harmony, which is thus a major concern for patients. When it comes to minor deformities, they have been treated with hyaluronic acid, toxin injection, and thread insertion since 2005. This study has originally been carried out to review the published medical literature on the non-surgical techniques for Medical Nose Reshaping (MNR): a PubMed search was carried out in December 2020 using the search terms: “Nose [and] filler”, “Nose [and] botulinum toxin” and “Nose [and] thread”. A large number of studies were identified and reviewed, and it was found that rhinoplasty remains the gold standard to correct nose defects; nevertheless, MNR is gaining popularity due to its minimal invasiveness, short downtime, and the market availability of different injectables, toxins, and threads. This study aims to provide an evidence-based approach to guide the use of non-surgical techniques for MNR by reviewing published medical literature and drawing on the authors’ experience.
CU(II)/Polydopamine-Modified Glass Fiber Separators Towards High-Performance Zinc-Ion Batteries
Authors: Ma Fengcan , Xie Kaixuan , Wu Siheng , Zhang Chi , Liao Xiaodie , Wang Qinghong
Published: June 2023

Much attention has been attracted by aqueous zinc zinc-ion batteries (AZIBs) due to the features of inherent safety, environmental compatibility, low cost and fantastic energy density. Nevertheless, chemical corrosion and dendrite growth occurring on Zn anode during the charge/discharge process usually cause the surface passivation and short circuit of cells, seriously hindering the development of AZIBs. To settle these problems, a Cu(II)/polydopamine modified glass fiber (Cu-PDA/GF) is designed as separator. Due to the enhanced ionic conductivity of Cu(II) and PDA modified glass fiber separator, reversible zinc plating/striping is achieved in symmetric cells, which display long cycle life of over 1800 h at the current density of 1 mA cm−2 with the fixed capacity of 1 mAh cm−2. Moreover, the assembled Zn//V2O5 cells using Cu-PDA/GF separator also demonstrate improved capacity retention. The present study provides a simple separator modification strategy for high-performance and reliable AZIBs, which are conducive to energy storage devices.
MUJERES EN EL SISTEMA INTERAMERICANO. SOBREVIVIENTES Y CONSTRUCTORAS DE LA JURISPRUDENCIA PROGRESISTA
Authors: Perez Padrón Diana
Published: June 2023

Esta obra contextualiza cual fue el rubmo de las dinamicas en los litigios donde las los papeles protagonicos fueron de mujeres valientes que buscaron la justicia que sus Estados no les dieron y al acudir a instancias supranacionales como la Corte Interamericana de Derechos Humanos. Ademas de recapitular todos esos procesos, el libro es un reconocimiento del trabajo de las mujeres en su camino al acceso a justicia, por lo que merce ser contado este precedente que como realidad vivieron las mujeres, una realidad de vulneraciones a derechos humanos que en países de América Latina parece ser la regla general.
LA DANZA DE LOS MATACHINES EN CIUDAD JUÁREZ: Prácticas y significaciones
Authors: CÓRDOVA ORTEGA DANIELA GUADALUPE
Published: June 2023

El presente libro trata sobre las danzas de matachines en Ciudad Juárez, Chihuahua, México. Su contexto en la ciudad está alimentado por distintos elementos que hacen de sí una práctica representativa y necesaria en variados actos de religiosidad popular. Carlo Bonfiglioli apunta que para el estudio de una danza se debe iniciar por describirla y ubicarla en su ámbito cultural, ya que es el reflejo de las correlaciones que se generan al interior de su cultura y que, en conjunto con las significaciones que se pueden derivar de dichos entramados, la danza conforma su propio sistema de relaciones semánticas. Visto de esta manera, en Ciudad Juárez las danzas ocupan un importante lugar en el entramado cultural, pues reflejan particularidades del contacto y relación de culturas que confluyen en momentos y en espacios diversos.
Deep Learning Analysis of Single-Cell Data Reveals Unique Genetic Features Of COVID-19 Severities
Authors: Schonfeld Elan
Published: June 2023

COVID-19 was declared by the World Health Organization in 2020 to be a pandemic. Analysis of COVID-19 related genetic pathways allows for a better understanding of the possible effects and sequelae of the disease. Using 6178 scRNA sequenced human cells, having a status of control/mild/severe COVID-19 disease status, differential expression of genes and pathways was analyzed. Using Gene Set Enrichment Analysis (GSEA), mild COVID-19 was found to over-express the Influenza Pathway. In order to identify genes important in COVID-19 severity, a deep learning classifier was trained. Classifiers were repeatedly trained for this task using 10 randomly selected genes from the total number of 18,958 genes. The highest performing classifier (AUC=0.748) was trained using: AC008626.1, SGO1, RHOBTB2, RBM41, NDUFAF4P1, COX5A, ZDHHC17, STX11, IPP, NUDT5 genes. These results further illustrate the other factors contributing to mild versus severe COVID-19, as well as evidence of potential misdiagnosis or overlapping pathway effects of Influenza and COVID-19.
Brief Tele-Mindfulness Based Intervention: A Randomized Controlled Multicenter Trial
Authors: AlQarni Amani M. , ElFaki Abdulaziz , Wahab Moataza M. Abdel , AlJehani Yasser , ALKhunaizi Auday A. , Alex Johnson , Othman Sharifa A. , Amer Fatma H. , AlGhamdi Faisal A. , Alissa Khalid A.
Published: June 2023

Background: To enhance psychological wellbeing of health care providers (HCP) who look after patients with COVID-19. This study is a psychological intervention in COVID-19 pandemic to check at what extent can interventions based on brief mindfulness-based interventions and progressive muscle relaxation affect psychological well-being, resilience, and anxiety of HCP.
Methods: This is a randomized trial study that was conducted from July to August 2020. 147 COVID-19 frontline HCP were randomized to a 2-week virtual intervention with brief mindfulness-based intervention (MBI) or to progressive muscle relaxation(PMR). Pre and post intervention assessment were done using the (state trait anxiety–20 Item Scale), the (Connor-Davidson Resilience Scale (CD-RISC-10), and WHO – 5 Well-Being Index.
Results: The two groups had significant improvement results in the psychological wellbeing and reduction of the state anxiety but not the trait anxiety nor the resiliency. Improvement was higher among group brief mindfulness-based intervention (81.3%) than among group progressive muscle relaxation (51.8%), (X2=12.9, p=0.0001), concerning psychological wellbeing.
Conclusions: Both brief mindfulness-based intervention and progressive muscle relaxation improved the psychological wellbeing and reduced the anxiety of frontline health care providers during COVID-19 pandemic with improvement being slightly higher among Brief mindfulness-based intervention.
Trial registration: Clinicaltrial.gov identifier: NCT04656626
https://clinicaltrials.gov/ct2/show/NCT04656626?cond=Mindfulness&cntry=SA&draw=2&rank=1
How can an inferior competitor be a successful invader?
Authors: Tesfay Yohannes B. , Blaschke Annika , Kreyling Jürgen
Published: June 2023

The invasion of ecosystems by non-native species is recognized as one of the most significant global challenges, particularly in semiarid regions where native biodiversity is already under stress from drought and land degradation. The implicit assumption is that invaders are strong competitors, but a greenhouse pairwise experiment conducted to examine intraspecific and interspecific competition effects of Opuntia ficus-indica, a widespread invader in semiarid ecosystems, with two species native to the highlands of Eritrea, Ricinus communis and Solanum marginatum, revealed that O. ficus-indica is a weak competitor. In direct interaction, the competition in aboveground productivity measured by the logarithmic response ratio for O. ficus-indica was 3.4-fold and 5.9-fold higher than for R. communis and S. marginatum, respectively. Belowground, the native R. communis was facilitated (-1.00±0.69) by O. ficus-indica which itself suffered from high competition. This pattern became even stronger under water shortage, where aboveground competition for S. marginatum decreased 5.7-fold, and for O. ficus-indica, it increased 1.4-fold. Despite being a poor competitor, O. ficus-indica outperformed R. communis and S. marginatum in both aboveground (4.3 and 3.8 times more) and belowground (27 and 2.8 times more) biomass production, respectively. The findings of this study challenge the common interpretation that invasive species are strong competitors and highlight the importance of considering other factors, such as productivity and tolerance limits, when assessing the potential impacts of invasive species on semiarid ecosystems.
Multi-jointed pneumatic sealing disc of fluid-driven pipeline robot: Impacts of structural parameters on performance
Authors: Yue Chunmei , Ma Qiang , Yang Zhenyuan , Liu Shuhai , Liu Shangjunnan
Published: June 2023

Pipeline inspection gauge (PIG) is often blockage in the aging pipelines of stacked objects owing to the limitation of its passive control. In this study, a novel sealing disc with multi-jointed pneumatic webbed foot was designed which can achieve partial active control and ensure better seal performance. A finite element method (FEM) was proposed and successfully verified, then the bend performance of the sealing disc with single inflated foot was compared with whole inflated feet using the FEM, and the results show that they have good consistency. Next, the effects of two kinds of materials and five structural parameters on the performance of the sealing disc were studied using the FEM. The results show that the multi-material structure has batter performance on the bending angle and the expansion ratio compared to the same materials. As the air pressure grows, the position parameter of the joint (he) has little effect on the bending angle as well as the expansion ratio, and the bottom disc thickness (hd) has also slightly influence on the expansion ratio. Meanwhile, the thicker the webbed foot thickness (ht), the smaller the slope angle (β) and the thinner (hd), the larger the bending angle; the smaller the slope angle, the thicker the webbed foot thickness, the larger the expansion ratio. The conclusions obtained in this paper are beneficial for the design and optimization of PIG sealing discs for actively controlling. The research results provide theoretical reference for the study of intelligent and efficient pipeline pigging technology.
Fallopian tube lesions as potential precursors of early ovarian cancer: A comprehensive proteomic analysis
Authors: Salzet Michel , Wisztorski Maxence , Aboulouard Soulaimane , Roussel Lucas , Duhamel Marie , saudemont Philippe , Tristan Cardon , Narducci Fabrice , Robin Yves-Marie , Lemaire Anne-Sophie , Bertin delphine , Hajjaji Nawale , Kobaissy Firas , Fournier Isabelle , Leblanc Eric
Published: June 2023

Ovarian cancer is the leading cause of death from gynecologic cancer worldwide. High-grade serous carcinoma (HGSC) is the most common and deadliest subtype of ovarian cancer. While the origin of ovarian tumors is still debated, it has been suggested that HGSC originates from cells in the fallopian tube epithelium (FTE), specifically the epithelial cells in the region of the tubal-peritoneal junction. Three main lesions, p53 signatures, STILs, and STICs, have been defined based on the immunohistochemistry (IHC) pattern of p53 and Ki67 markers and the architectural alterations of the cells, using the Sectioning and Extensively Examining the Fimbriated End Protocol. In this study, we performed an in-depth proteomic analysis of these pre-neoplastic epithelial lesions guided by mass spectrometry imaging and IHC. We evaluated specific markers related to each preneoplastic lesion. The study identified specific lesion markers, such as CAVIN1, Emilin2, and FBLN5. We also used SpiderMass technology to perform a lipidomic analysis and identified the specific presence of fatty acid synthase (FASN) and fatty aldehyde dehydrogenase (FADH) with specific lipids in p53 lesions. Our study provides new insights into the molecular mechanisms underlying the progression of ovarian cancer and confirms the fimbria origin of HGSC.
Genetic modulation of brain dynamics in neurodevelopmental disorders: the impact of copy number variations on resting-state EEG
Authors: Audet-Duchesne Elisabeth , Knoth Inga , Martin Charles-Olivier , Jizi Khadije , Tamer Petra , Younis Nadine , Jacquemont Sébastien , Dumas Guillaume , Lippé Sarah
Published: June 2023

Research has shown that many copy number variations (CNVs) increase the risk of neurodevelopmental disorders (e.g., autism, ADHD, schizophrenia). However, little is known about the effects of CNVs on brain development and function. Resting-state electroencephalography (EEG) is a suitable method to study the disturbances of neuronal functioning in CNVs. We aimed to determine whether there are resting-state EEG signatures that are characteristic of children with pathogenic CNVs. EEG resting-state brain activity of 109 CNVs carriers (66 deletion carriers, 43 duplication carriers) aged 3 to 17 years was recorded for 4 minutes. To better account for developmental variations, EEG indices (power spectral density and functional connectivity) were corrected with a normative model estimated from 256 Healthy Brain Network controls. Results showed increased beta and gamma power in posterior regions as well as global under-connectivity in several frequency bands in CNVs carriers. Deletion and duplication carriers can be differentiated by their connectivity in low alpha frequencies: duplication carriers connectivity was more disrupted than deletion carriers. The distinctive connectivity perturbations were found to be most prominent during adolescence. The results suggest that CNVs carriers show electrophysiological alterations compared to neurotypical controls, regardless of the gene dosage effect and of their affected genomic region. Moreover, a specific signature of the brain alterations associated with duplications was found.
Hospital vulnerability to spread of respiratory infections: close contact data collection and mathematical modelling
Authors: Shirreff George , Huynh Bich-Tram , Duval Audrey , Pereira Lara Cristina , Annane Djillali , Dinh Aurélien , Lambotte Olivier , Bulifon Sophie , Guichardon Magali , Beaune Sebastien , Toubiana Julie , Kermorvant-Duchemin Elsa , Chéron Gerard , Cordel Hugues , Argaud Laurent , Douplat Marion , Abraham Paul , Tazarourte Karim , Martin-Gaujard Géraldine , Vanhems Philippe , Hilliquin Delphine , Nguyen Duc , Chelius Guillaume , Fraboulet Antoine , Temime Laura , Opatowski Lulla , Guillemot Didier
Published: June 2023

The transmission risk of SARS-CoV-2 within hospitals can exceed that in the general community because of more frequent close proximity interactions. However, epidemic risk across wards is still poorly described. We measured CPIs directly using wearable sensors given to all those present in a clinical ward over a 36-hour period, across 15 wards in three hospitals in spring 2020. Data were collected from 2114 participants. These data were combined with a simple transmission model describing the arrival of a single index case to the ward to estimate the risk of an outbreak. Estimated epidemic risk ranged four-fold, from 0.12 secondary infections per day in an adult emergency to 0.49 per day in general paediatrics. The risk presented by an index case in a patient varied twenty-fold across wards. Using simulation, we assessed the potential impact on outbreak risk of targeting the most connected individuals for prevention. We found that targeting those with the highest cumulative contact hours was most impactful (20% reduction for 5% of the population targeted), and on average resources were better spent targeting patients. This study reveals patterns of interactions between individuals in hospital during a pandemic and opens new routes for research into airborne nosocomial risk.
Financial characteristics and security of podiatry work in Victoria: the PAIGE cross sectional study of Australian podiatrists
Authors: Couch Anna Grace , Haines Terry , O'Sullivan Belinda , Menz Hylton B , Williams Cylie M
Published: June 2023

Background
Podiatrists’ earnings have an important influence on workforce dynamics. This includes the profession’s ability to retain workers so the populations healthcare needs can be met. This study aimed to describe factors relating to Victorian podiatrists’ financial characteristics and security.
Methods
This was a cross sectional study using data from Victorian podiatrists who participated in Wave 1 of the Podiatrists in Australia: Investigating Graduate Employment (PAIGE) survey. Demographic and financial characteristics were described. The outcome measure, financial security, was collected through a self-reported belief based on current financial situation and prospects, respondents perception of having enough income to live on when they retire. Univariate logistic regression was used to determine associations with rural or metropolitan practice locations. Multiple ordered logistic regression was performed to explore associations between factors relating to financial security and retirement prospects.
Results
There were 286 Victorian podiatrist (18% of n = 1,585 Victorian podiatrists) respond. Of these, 206 (72% of n = 286) identified as female, 169 (59% of 286) worked in the private sector and the mean (SD) age was 33.4 (9.5) years. The mean (SD) annual gross income was $79,194 ($45,651) AUD, and 243 (87% of 279) made regular superannuation contributions. Multiple ordered logistic regression analyses identified factors associated with podiatrists’ perception of having adequate retirement income. These included being an owner/partner of their main workplace (adj OR = 2.70, 95% CI = 1.49–4.76), growing up in a rural location (adj OR = 2.27, 95% CI = 1.38–3.70), perceiving a moderate overall health rating (adj OR = 2.03 95% CI = 1.51–2.75), not having financial debt related to education and training (adj OR = 2.02, 95% CI = 1.24–3.32) and regular contributions to a superannuation scheme (adj OR = 4.76, 95% CI = 2.27-10.00).
Conclusion
This is the first known study to explore podiatrists’ earnings and perceptions regarding financial security for retirement. Findings suggest modifiable ways to improve financial security of podiatrists including support and education about personal and business finances including debt management and understanding the importance of contributions to superannuation when self-employed, or skills and supports for podiatrists to run their own businesses. This research is exploratory and is relevant for understanding the impact that income and financial security have on workforce capacity.
Metabolomics and machine learning technique reveals that germination enhances the multi-nutritional properties of pigmented rice
Authors: Fernie Alisdair
Published: June 2023

Enhancing the dietary properties of rice is crucial to contribute to alleviating hidden hunger and non-communicable diseases in rice-consuming countries. Germination is a bioprocessing approach to increase the nutritional properties of seeds. However, there is scarce information on how germination impacts the overall nutritional profile of pigmented rice sprouts. Herein, we demonstrated that germination could increase certain dietary compounds, such as phenolics, GABA, and micronutrients, and induce new flavonoid glycosides. Minerals such as Ca, Na, Fe, Zn, and vitamins such as riboflavin and biotin increased upon germination. In addition, this process decreased toxic elements like aluminum. This process allowed for a decrease in bound phenolics leading to an increase in free phenolics. Metabolomic analysis revealed the preferential accumulation of flavonoid compounds in the germination process. Genome-wide association studies of the altered metabolites revealed the activation of specific genes responsible for increasing certain flavonoid compounds. Notably, the activation of the CHS1 gene boosted the naringenin and the compounds along this pathway. Likewise, the UGT gene is responsible for the formation of flavonoid glycosides derived from kaempferol, caffeic acid, ferulic acid, and quercetin. Haplotype analyses showed a significant difference (P < 0.05) between alleles associated with this genetic region. Genetic markers associated with these flavonoids were incorporated into the random forest model, improving the accuracy from 89.7% to 97.7%. Consistent with this feature, the improved model has a faster prediction speed in a shorter training time. The elite cultivars can breed rice with multi-nutritional properties, and the model could screen rice with several dietary benefits.
The influence of organic components in sludge on selective non-catalytic reduction denitrification
Authors: Wang Yali , Zong Xiaotong , Song Yihui , Cui Suping
Published: June 2023

Collaborative disposal of sludge has been applied extensively in cement industry kilns，but the influence of organic components in sludge on the SNCR denitrification is still in highly demanding. This paper focuses the influence of ethanol, n-octane, benzene and acetone on the SNCR denitrification, and the influence of ethanol are analyzed on SNCR denitrification under different flue gas condition. The result showed that ·H and ·OH as active groups are produced during the combustion of organic components, promoting the generation of ·NH2 from NH3, and therein the lower reaction temperature of SNCR. When the temperature of SNCR reaction is at 750~800℃, the denitrification rate of four organic components is: ethanol > benzene > n-octane > acetone. The optimum denitrification rate on SNCR can reach as high as 90.377% when the reaction condition is: the NH3/NO is 1, the ethanol concentration is 1000ppm, O2 concentration is 2% and temperature is 750℃. In this paper, the influence of organic components in sludge on SNCR denitrification will be analyzed, which provides a theoretical basis for the selection of sludge dosing method for cement kiln collaborative disposal.
ENCOURAGEing Start: A Quasi-experimental Trial on Pre-Op Physical Activity Intervention for Bariatric Surgery Candidates
Authors: Edye-Mazowita Alex , He Wenjing , Duhamel Todd A. , Hardy Krista , Vergis Ashley
Published: June 2023

Introduction
The American Society for Metabolic and Bariatric Surgery recommends pre- and post-operative physical activity (PA). However, there are no effective standardized PA programs incorporating a behavioral change approach for bariatric surgery patients. A novel pre-operative PA intervention ENCOURAGEing Start was developed and piloted to assist patients in reducing barriers to PA and encouraging positive PA habits.
Methods
This single arm pilot study recruited patients (n=27, BMI = 47.1 ± 6.2) from the Victoria General Hospital’s Centre for Metabolic and Bariatric Surgery in Winnipeg, Manitoba between September 2017 and May 2018. A time-series quasi-experimental design was used. The 16-week intervention took place at the University of Manitoba’s Active Living Centre. The primary outcome was a six-minute walk test (6MW), a measure of functional ability and predictive of post-surgical weight loss and maintenance. Secondary outcomes included fitness, cardiovascular disease risk and mental health. Outcomes were evaluated at baseline, 8 weeks, and 16 weeks.
Results
Ten patients completed the intervention. Clinically significant (p≤0.05) differences in the 6MW (457.21 vs 497.58 m, p=0.002), indirect 1RM leg press (283.72 vs 331.60 lb, p<0.001), sit-to-stand (12.78 vs 14.82, p<0.01), and quality of life (181.89 vs 195.96, p=0.028) were identified for completers.
Conclusions
The ENCOURAGEing Start intervention enhanced markers linked to long-term post-surgery outcomes, including a 10.7% increase in 6MW. This feasible program showed similar effect size to more resource intensive interventions, including those in Canada. A larger study with a minimum of 55 participants should be undertaken to assess the sustainability of these effects.
Microsurgical Training Curriculum in a gynecological breast cancer center – a benefit for patients and surgeons?
Authors: Schmidt Georg Philipp , Mayo Theresa , Paepke Stefan , Kiechle Marion , Müller Daniel
Published: June 2023

Purpose
Autologous breast reconstruction improves patient satisfaction and quality of life after mastectomy. In Germany, free flap surgery and implant-based reconstruction is usually separate between reconstructive surgery and gynaecology. Cooperation between the specialist disciplines and implementation of microsurgery into breast surgeon training could enhance surgical treatment for breast cancer patients.
Methods
At the breast cancer center at Klinikum rechts der Isar, TU Munich, a three-stage training program for autologous breast reconstruction and microsurgery for gynecological breast surgeons was developed. Between 2019 and 2022 74 women received autologous free flap breast reconstruction by a consistent team consisting of a gynaecological surgeon in training and an expert microsurgeon. Peri- and postoperative data were collected to analyse the feasibility and safety of a microsurgical training in gynaecology.
Results
Within the training, operative steps of free autologous breast reconstruction were increasingly taken over by the gynaecological surgeon in training. The analysis showed a decrease in operating times with consistently low complication rates during the training.
Conclusion
This study demonstrated that a training in free autologous breast reconstruction for gynaecological surgeons is safely feasible through close cooperation between gynaecological and reconstructive surgery.
The Importance of Facilitating Goal-Concordant Care (GCC) in a Pandemic: The MD Anderson Experience with hospitalized COVID-19 positive patients
Authors: Muthu Mayoora , Dalal Shalini , George Marina , Clavijo Cesar Simbaqueba , Lenz Caitlin , Nortje Nico
Published: June 2023

Purpose Provider-patient communication (PPC) about goals of care (GOC) facilitates goal-concordant care (GCC) delivery. Hospital resource limitations imposed during the pandemic made it vital to deliver GCC to a patient cohort with COVID-19 and cancer. Our aim was to understand the population and adoption of GOC-PPC along with structured documentation in the form of an Advance Care Planning (ACP) note.Methods A multidisciplinary GOC task force developed processes for ease of conducting GOC-PPC and implemented structured documentation. Data were obtained from multiple electronic medical record elements, with each source identified, data integrated and analyzed. We looked at PPC and ACP documentation pre and post implementation alongside demographics, length of stay (LOS), 30-day readmission rate and mortality.Results 494 unique patients were identified, 52% male, 63% Caucasian, 28% Hispanic, 16% African American and 3% Asian. Active cancer was identified in 81% patients, of which 64% were solid tumors and 36% hematologic malignancies. LOS was 9 days with a 30-day readmission rate of 15% and inpatient mortality of 14%. Inpatient ACP note documentation was significantly higher post-implementation as compared to pre-implementation (90% vs 8%, P < 0.05). We saw sustained ACP documentation throughout the pandemic suggesting effective processes.Conclusions The implementation of institutional structured processes for GOC-PPC resulted in rapid sustainable adoption of ACP documentation for COVID-19 positive cancer patients. This was highly beneficial for this population during the pandemic, as it demonstrated the role of agile processes in care delivery models, which will be beneficial in the future when rapid implementation is needed.
Surgical Outcomes of Percutaneous Endoscopic Lumbar Discectomy in Obese Adolescents with Lumbar Disc Herniation
Authors: Qu Lianjun , Wang Yongli , Wang Fei , Zhang Songou
Published: June 2023

In recent years, with improved living standards, adolescent obesity has been widely studied. The lumbar disc herniation (LDH) incidence in obese adolescents is increasing yearly. No clinical studies have reported the use of percutaneous endoscopic lumbar discectomy (PELD) in obese adolescent lumbar disc herniation (ALDH) patients. This study evaluated the preliminary surgical outcomes of PELD treatment of obese ALDH patients. Fifty-one ALDH patients underwent single-level PELD surgery between January 2014 and January 2020. Patients were divided into an obese group and a normal group. Patient characteristics and surgical variables were compared between the two groups. The VAS, ODI, and SF-36 scales were used to evaluate the preoperative and postoperative clinical efficacy. In this study, 19 patients were included in the obese group, and 28 were included in the normal group. There was no significant difference in age, sex, duration of low back pain, duration of leg pain, or operative level between the obese and normal groups preoperatively. The obese group had a longer operative time (OT) (101.9 ± 9.0 min vs. 84.3 ± 11.0 min, P < 0.001) and greater fluoroscopy times (41.0 ± 5.8 vs. 31.6 ± 7.0, P < 0.001) and a later time of ambulation (29.9 ± 4.0 vs. 25.0 ± 2.9, p < 0.001) than the normal group. The groups did not significantly differ in complications. The VAS score for back and leg pain and the ODI and SF-36 scores for functional status improved significantly postoperatively. The PELD procedure is a safe and feasible method for treating LDH in obese adolescents. Obese ALDH requires a longer OT, a larger fluoroscopy dose and a later timepoint for getting out of bed than normal ALDH. However, PELD yields similar clinical outcomes between obese and normal ALDH patients.
Placentome Size in Bos Taurus X Bos Indicus Crosses in the Mexican Tropics Differs From the Source Breeds
Authors: Bautista Nectalí Mendoza , Saavedra-Jiménez Luis Antonio , Sánchez-Santillán Paulino , Bottini-Luzardo Maria
Published: June 2023

Placentome size in cows can vary in response to many factors, including crossbreeding. We measured placentome size at different gestational ages in Bos taurus x Bos indicus cows raised in the tropics. Uteruses were collected immediately after slaughter from 102 cows with gestations ≥ 30 days and processed in the abattoir. All cows were Swiss Brown x Zebu phenotypes. Fetus gestational age was estimated by measuring from the crown to the tail base. Five placentomes were randomly chosen from each uterus (n = 510), and diameter (cm) measured for each. Gestational age was classified into nine categories (days): a) ≤ 45, b) > 45 ≤ 60, c) > 60 ≤ 90, d) > 90 ≤ 120, e) > 120 ≤ 150, f) > 150 ≤ 180, g) > 180 ≤ 210, h) > 210 ≤ 240 and i) > 240. Placentome size data was grouped by these categories. In all nine categories, placentome size was larger than reported for Bos taurus cows and smaller than reported in Bos indicus cows. This intermediate size suggests that existing gestational age reference tables based on placentome size for purebred cows are not applicable to Bos taurus x Bos indicus crosses.