bluuebunny/crossref_metadata_2025 huggingface dataset:
Leaf morphology and anatomy of Schlechtendalia luzulifolia, a basal member of subfamily Barnadesioideae (Asteraceae)
Authors: Urtubey Estrella , Klusch María C , Stuessy Tod F
Published: August 2023

Based on molecular phylogenetic studies, Barnadesioideae have been proposed to be the basal subfamily of Asteraceae. This is a complex of 10 genera and 87 species distributed primarily along the Andean mountains, Patagonia, and into southern Brazil and Uruguay. Phylogenetic analyses have recovered all genera as monophyletic groups and have provided insights to their inter-relationships. Four generic clades have been substantiated: (1) Chuquiraga, Doniophyton, and Duseniella; (2) Dasyphyllum; (3) Barnadesia and Huarpea; and (4) Archidasyphyllum, Arnaldoa, and Fulcaldea. The remaining genus, the monospecific Schlechtendalia, has been an outlier in the subfamily, with some previous analyses recovering it as basal for the entire subfamily, and others showing it as sister to Barnadesia and Huarpea (with weak support) as well as to other genera. Recent massive sampling of loci has confirmed Schlechtendalia as the sister genus for the subfamily. Schlechtendalia luzulifolia has morphology atypical for Asteraceae. The capitula are loose aggregations of florets, and the leaves are long and strap shaped, more reminiscent of monocots. Morphological and anatomical investigations of the leaves reveal long, laminar blades with parallelodromous vascularization. The vesture is often with ‘barnadesioid trichomes’, especially towards the base of the plant, plus additional uniseriate trichomes consisting of 3 to many cells, newly reported for the subfamily. Some glandular trichomes with 2-4 short cells also occur. The transverse anatomy of the leaves reveals a single epidermal layer on both surfaces, which also contain the stomata (the leaf being amphistomatic). The mesophyll is undifferentiated; the vascular traces are surrounded by sclerenchyma that not only encircles the traces but also extends towards the epidermis and connects with it. The morphology and anatomy of the leaves of Schlechtendalia are divergent in comparison with other genera of the subfamily. Chuquiraga, Doniophyton, and Huarpea have leaf adaptations for survival in xeric habitats, such as dense pubescence, grey surfaces, and revolute margins. Schlechtendalia, in contrast, is adapted to a more mesic environment, especially near the Atlantic Ocean and along the Uruguay and La Plata rivers. The leaves are oriented upright, which correlates with undifferentiated mesophyll and stomata on both epidermal layers. The stem is an underground rhizome, an adaptation that permits survival during seasonal drought in the austral summer in Uruguay and adjacent regions. It is hypothesized that Schlechtendalia may have become adapted to more mesic environments in the Miocene prior to the rise of the Andes and development of the modern arid environments, into which many of the other genera of the subfamily subsequently radiated.
“Do We Stay or Do We Go?” The Impact of Anti-LGBTQ+ Legislation on the HIV Workforce in the South
Authors: Person Anna K , Terndrup Christopher P , Jain Mamta K , Kelley Colleen F
Published: August 2023

The human immunodeficiency virus (HIV) workforce continues to face a crisis, particularly in the southern United States. Adding to known issues of administrative burden and less competitive compensation, recent anti- lesbian, gay, bisexual, transgender and queer (LGBTQ+) legislation threatens the already strained HIV workforce. HIV care providers advocate for all aspects of their patient's lives, including those needing gender-affirming care. The recent legislative targets against transgender patients, which involves many people with HIV, will clearly add to the burden on individual HIV care providers and therefore the HIV workforce. Recruitment and retention efforts in states impacted by these laws will become increasingly difficult without advocacy for the patients we serve. The HIV workforce must work together with LGBTQ+ populations to address these recent laws and promote the well-being of all our patients and colleagues.
Nirmatrelvir Resistance—de Novo E166V/L50V Mutations in an Immunocompromised Patient Treated With Prolonged Nirmatrelvir/Ritonavir Monotherapy Leading to Clinical and Virological Treatment Failure—a Case Report
Authors: Zuckerman Neta S , Bucris Efrat , Keidar-Friedman Danielle , Amsalem Muriel , Brosh-Nissimov Tal
Published: August 2023

Resistance of SARS-CoV-2 to antivirals was shown to develop in immunocompromised individuals receiving remdesivir. We describe an immunocompromised patient who was treated with repeated and prolonged courses of nirmatrelvir and developed de-novo E166V/L50F mutations in the Mpro region. These mutations were associated with clinical and virological treatment failure.
Dual mechanical valve thrombosis resolution through thrombolytics: a case series
Authors: McGuire Colin , Yang Michael , Papolos Alexander , Rogers Toby , Kenigsberg Benjamin
Published: August 2023

Background

Prosthetic valve thrombosis (PVT) is a severe complication of mechanical valve replacement. Simultaneous thrombosis of multiple prosthetic valves is rare and is associated with worse outcomes. Treatment options include anticoagulation, thrombolysis, and redo operative valve replacement, with rare reports of adjunctive balloon valvuloplasty. There is limited evidence to guide therapeutic selection, specifically dosing, timing, and duration of thrombolysis. The following case series highlights the importance of successful thrombolytic management in dual PVT with high bleeding risk defined as a coagulopathy with an elevated international normalized ratio greater than 3 and New York Heart Association (NYHA) Class III and IV heart failure.

Case summary

We describe two patients with concomitant aortic and mitral PVT. Both patients presented in NYHA Class III and IV heart failure with different challenges to surgical treatments including high bleeding risk from coagulopathy and history of multiple prior sternotomies. After multi-disciplinary discussions, both patients underwent a combination of low-dose, slow, or ultra-slow infusion of tissue plasminogen activator, with a resolution of their dual PVT seen on cine-fluoroscopy imaging as freely moving mechanical leaflets and improvement of heart failure symptoms back to baseline NYHA Class II or lower.

Discussion

Prosthetic valve thrombosis is a complex medical condition requiring a multi-disciplinary team to evaluate the best course of treatment. A trial of pharmacologic thrombolysis is often the first attempted treatment for obstructive PVT, although surgery is recommended for patients with NYHA IV symptoms, or with contraindications to thrombolysis, including high bleeding risk. However, in patients with high bleeding risk and NYHA Class III and IV heart failure, especially with surgical contraindications, low-dose thrombolytics, with slow or ultra-slow infusions, may still be safe and effective treatment strategies for multi-valve PVT. Further research is needed to guide thrombolysis in multi-valve PVT.
Digest: Unveiling the role of chemical communication in lizard diversification
Authors: Román-Palacios Cristian
Published: August 2023

Although many studies looking at the role that sexual selection plays in macroevolution have focused on the involvement of visual and acoustic signals, the potential influence of chemical communication remains unexplored. Instead of focusing on well-studied instances of sexually selected traits, Murali et al. analyze chemical communication to test whether sexual selection could help explain large-scale species diversity patterns in lizards. Their results suggest that, at least in lizards, chemical communication is uncoupled from differential diversification dynamics across clades.
Praemonitus praemunitus: can we forecast and prepare for future viral disease outbreaks?
Authors: Sessions Zoe , Bobrowski Tesia , Martin Holli-Joi , Beasley Jon-Michael T , Kothari Aneri , Phares Trevor , Li Michael , Alves Vinicius M , Scotti Marcus T , Moorman Nathaniel J , Baric Ralph , Tropsha Alexander , Muratov Eugene N
Published: August 2023

Understanding the origins of past and present viral epidemics is critical in preparing for future outbreaks. Many viruses, including SARS-CoV-2, have led to significant consequences not only due to their virulence, but also because we were unprepared for their emergence. We need to learn from large amounts of data accumulated from well-studied, past pandemics and employ modern informatics and therapeutic development technologies to forecast future pandemics and help minimize their potential impacts. While acknowledging the complexity and difficulties associated with establishing reliable outbreak predictions, herein we provide a perspective on the regions of the world that are most likely to be impacted by future outbreaks. We specifically focus on viruses with epidemic potential, namely SARS-CoV-2, MERS-CoV, DENV, ZIKV, MAYV, LASV, noroviruses, influenza, Nipah virus, hantaviruses, Oropouche virus, MARV, and Ebola virus, which all require attention from both the public and scientific community to avoid societal catastrophes like COVID-19. Based on our literature review, data analysis, and outbreak simulations, we posit that these future viral epidemics are unavoidable, but that their societal impacts can be minimized by strategic investment into basic virology research, epidemiological studies of neglected viral diseases, and antiviral drug discovery.
The Association Between Ankle–Brachial Index and Daily Patterns of Physical Activity: Results From the Hispanic Community Health Study/Study of Latinos
Authors: Chiu Venus , Urbanek Jacek K , Wanigatunga Amal A , Allison Matthew A , Ballew Shoshana H , Mossavar-Rahmani Yasmin , Sotres-Alvarez Daniela , Gallo Linda C , Xue Xiaonan , Talavera Gregory A , Evenson Kelly R , Kaplan Robert C , Matsushita Kunihiro , Schrack Jennifer A
Published: August 2023

Background

Peripheral artery disease (PAD) is associated with lower physical activity but less is known about its association with daily patterns of activity. We examined the cross-sectional association between ankle–brachial index (ABI) and objectively measured patterns of physical activity among Hispanic/Latino adults.

Methods

We analyzed data from 7 688 participants (aged 45–74 years) in the Hispanic Community Health Study/Study of Latinos. ABI was categorized as low (≤0.90, indicating PAD), borderline low (0.91–0.99), normal (1.00–1.40), and high (>1.40, indicating incompressible ankle arteries). Daily physical activity metrics derived from accelerometer data included: log of total activity counts (LTAC), total log-transformed activity counts (TLAC), and active-to-sedentary transition probability (ASTP). Average differences between ABI categories in physical activity, overall and by 4-hour time-of-day intervals, were assessed using linear regression and mixed-effects models, respectively.

Results

In Hispanic/Latino adults, 5.3% and 2.6% had low and high ABIs, respectively. After adjustment, having a low compared to a normal ABI was associated with lower volume (LTAC = −0.13, p < .01; TLAC = −74.4, p = .04) and more fragmented physical activity (ASTP = 1.22%, p < .01). Having a low ABI was linked with more fragmented physical activity after 12 pm (p < .01). Having a high ABI was associated with lower volumes of activity (TLAC = −132.0, p = .03).

Conclusions

Having a low or high ABI is associated with lower and more fragmented physical activity in Hispanic/Latino adults. In adults with low ABI, physical activity is more fragmented in the afternoon to evening. Longitudinal research is warranted to expand these findings to guide targeted interventions for PAD or incompressible ankle arteries.
Worsened Ability to Engage in Social and Physical Activity During the COVID-19 Pandemic and Older Adults’ Mental Health: Longitudinal Analysis From the Canadian Longitudinal Study on Aging
Authors: Cosco Theodore D , Wister Andrew , Best John R , Riadi Indira , Kervin Lucy , Hopper Shawna , Basta Nicole E , Wolfson Christina , Kirkland Susan A , Griffith Lauren E , McMillani Jacqueline M , Raina Parminder
Published: August 2023

Background and Objectives

Restrictions implemented to mitigate the transmission of coronavirus disease 2019 (COVID-19) affected older adults’ ability to engage in social and physical activities. We examined mental health outcomes of older adults reporting worsened ability to be socially and physically active during the pandemic.

Research Design and Methods

Using logistic regression, we examined the relationship between positive screen for depression (10-item Center for Epidemiological Studies—Depression Scale) or anxiety (7-item Generalized Anxiety Scale) at the end of 2020 and worsened ability to engage in social and physical activity during the first 6–9 months of the pandemic among older adults in Canada. Interactions between ability to participate in social and physical activity and social participation pre-COVID (2015–2018) and physical activity were also examined. We analyzed data collected before and during the COVID pandemic from the Canadian Longitudinal Study on Aging, a nationally representative longitudinal cohort: pre-pandemic (2015–2018), COVID-Baseline survey (April to May 2020), and COVID-Exit survey (September to December 2020).

Results

Of the 24,108 participants who completed the COVID-Exit survey, 21.96% (n = 5,219) screened positively for depression and 5.04% (n = 1,132) for anxiety. Worsened ability to participate in social and physical activity was associated with depression (odds ratio [OR] = 1.85 [95% confidence interval {CI} 1.67–2.04]; OR = 2.46 [95% CI 2.25–2.69]), respectively, and anxiety (OR = 1.66 [95% CI 1.37–2.02] and OR = 1.96 [95% CI 1.68–2.30]). Fully adjusted interaction models identified a buffering effect of social participation and the ability to participate in physical activity on depression (χ2 [1] = 8.86, p = .003 for interaction term).

Discussion and Implications

Older adults reporting worsened ability to participate in social and physical activities during the COVID-19 pandemic had poorer mental health outcomes than those whose ability remained the same or improved. These findings highlight the importance of fostering social and physical activity resources to mitigate the negative mental health impacts of future pandemics or other major life stressors that may affect the mental health of older adults.
Theoretical study of low-GWP refrigerants in high-temperature heat pump systems
Authors: Zhou Dong , Meng Zhaofeng , Liu Yin , Dong Suiju , Zhang Fan , Ding Chuangchuang , Huo Ziheng , Wang Mingming
Published: August 2023

In this paper, the thermal performance of three low-global warming potential refrigerants R1224yd(Z), R1223zd(E) and R1336mzz(Z) were studied to replace R245fa in the high-temperature heat pump system. The results showed that the heating capacity of R1224yd(Z), R1223zd(E) and R1336mzz(Z) was 6, 12 and 88% less than that of R245fa, respectively. The coefficient of performance (COP) of R1224yd(Z) and R1223zd(E) was 2 and 1% higher than that of R245fa, respectively. However, the COP of R1336mzz(Z) was 5% less than that of R245fa. The difference in compressor discharge temperature between R1224yd(Z), R1223zd (E), R1336mzz(Z) and R245fa was about ±5°C. R1224yd(Z) is the best refrigerant to replace R245fa. R1223zd(E) is the second. R1336mzz(Z) cannot be directly replaced unless the original system is modified.
Perspectives on Advancing Countermeasures for Filovirus Disease: Report From a Multisector Meeting
Authors: Sprecher Armand , Cross Robert , Marzi Andrea , Martins Karen A , Wolfe Daniel , Montgomery Joel M , Spiropoulou Christina F , Cihlar Tomas , Ahuka-Mundeke Steve , Nyhuis Tara , Teicher Carrie , Crozier Ian , Strong Jim , Kobinger Gary , Woolsey Courtney , Geisbert Thomas W , Feldmann Heinz , Muyembe Jean-Jacques
Published: August 2023

Although there are now approved treatments and vaccines for Ebola virus disease, the case fatality rate remains unacceptably high even when patients are treated with the newly approved therapeutics. Furthermore, these countermeasures are not expected to be effective against disease caused by other filoviruses. A meeting of subject-matter experts was held during the 10th International Filovirus Symposium to discuss strategies to address these gaps. Several investigational therapeutics, vaccine candidates, and combination strategies were presented. The greatest challenge was identified to be the implementation of well-designed clinical trials of safety and efficacy during filovirus disease outbreaks. Preparing for this will require agreed-upon common protocols for trials intended to bridge multiple outbreaks across all at-risk countries. A multinational research consortium including at-risk countries would be an ideal mechanism to negotiate agreement on protocol design and coordinate preparation. Discussion participants recommended a follow-up meeting be held in Africa to establish such a consortium.
Modeling Poliovirus Transmission and Responses in New York State
Authors: Thompson Kimberly M , Kalkowska Dominika A , Routh Janell A , Brenner I Ravi , Rosenberg Eli S , Zucker Jane R , Langdon-Embry Marisa , Sugerman David E , Burns Cara C , Badizadegan Kamran
Published: August 2023

Background

In July 2022, New York State (NYS) reported a case of paralytic polio in an unvaccinated young adult, and subsequent wastewater surveillance confirmed sustained local transmission of type 2 vaccine-derived poliovirus (VDPV2) in NYS with genetic linkage to the paralyzed patient.

Methods

We adapted an established poliovirus transmission and oral poliovirus vaccine evolution model to characterize dynamics of poliovirus transmission in NYS, including consideration of the immunization activities performed as part of the declared state of emergency.

Results

Despite sustained transmission of imported VDPV2 in NYS involving potentially thousands of individuals (depending on seasonality, population structure, and mixing assumptions) in 2022, the expected number of additional paralytic cases in years 2023 and beyond is small (less than 0.5). However, continued transmission and/or reintroduction of poliovirus into NYS and other populations remains a possible risk in communities that do not achieve and maintain high immunization coverage.

Conclusions

In countries such as the United States that use only inactivated poliovirus vaccine, even with high average immunization coverage, imported polioviruses may circulate and pose a small but nonzero risk of causing paralysis in nonimmune individuals.
Reporting England’s progress towards the ambitions in the UK action plan for antimicrobial resistance: the English surveillance programme for antimicrobial utilisation and resistance (ESPAUR)
Authors: Ashiru-Oredope Diane , Cunningham Neil , Casale Ella , Muller-Pebody Berit , Hope Russell , Brown Colin S , Hopkins Susan
Published: August 2023

The plans for a new antimicrobial utilization and resistance national surveillance programme, alongside the development of quality measures and methods to monitor unintended outcomes of antimicrobial stewardship and both public and professional behaviour interventions were published in 2013. Since then, England has published an annual surveillance report including outlining progress against the ambitions of the UK national action plans on antimicrobial resistance (2013 to 2018 and 2019 to 2024). A decade later we provide a brief update on progress so far, with a focus on key highlights from the latest report published in November 2022. We also provide our recommendations for areas of focus as we move into the next decade. From an initial focus on antibiotic consumption and resistance, the report now includes surveillance data for antifungals, antivirals (including novel agents, such as those targeting SARS-CoV-2) and antimalarials. Evaluation of key stewardship interventions including professional and public engagement initiatives are also reported, as well as progress against NHS England’s (NHSE’s) improvement measures.
Clinical safety and efficacy of novel antifungal, fosmanogepix, for the treatment of candidaemia: results from a Phase 2 trial
Authors: Pappas Peter G , Vazquez Jose A , Oren Ilana , Rahav Galia , Aoun Mickael , Bulpa Pierre , Ben-Ami Ronen , Ferrer Ricard , Mccarty Todd , Thompson George R , Schlamm Haran , Bien Paul A , Barbat Sara H , Wedel Pamela , Oborska Iwona , Tawadrous Margaret , Hodges Michael R
Published: August 2023

Background

Fosmanogepix is a first-in-class antifungal targeting the fungal enzyme Gwt1, with broad-spectrum activity against yeasts and moulds, including multidrug-resistant fungi, formulated for intravenous (IV) and oral administration.

Methods

This global, multicenter, non-comparative study evaluated the safety and efficacy of fosmanogepix for first-line treatment of candidaemia in non-neutropenic adults. Participants with candidaemia, defined as a positive blood culture for Candida spp. within 96 h prior to study entry, with ≤2 days of prior systemic antifungals, were eligible. Participants received fosmanogepix for 14 days: 1000 mg IV twice daily on Day 1, followed by maintenance 600 mg IV once daily, and optional switch to 700 mg orally once daily from Day 4. Eligible participants who received at least one dose of fosmanogepix and had confirmed diagnosis of candidaemia (<96 h of treatment start) composed the modified intent-to-treat (mITT) population. Primary efficacy endpoint was treatment success at the end of study treatment (EOST) as determined by the Data Review Committee. Success was defined as clearance of Candida from blood cultures with no additional antifungal treatment and survival at the EOST.

Results

Treatment success was 80% (16/20, mITT; EOST) and Day 30 survival was 85% (17/20; 3 deaths unrelated to fosmanogepix). Ten of 21 (48%) were switched to oral fosmanogepix. Fosmanogepix was well tolerated with no treatment-related serious adverse events/discontinuations. Fosmanogepix had potent in vitro activity against baseline isolates of Candida spp. (MICrange: CLSI, 0.002–0.03 mg/L).

Conclusions

Results from this single-arm Phase 2 trial suggest that fosmanogepix may be a safe, well-tolerated, and efficacious treatment for non-neutropenic patients with candidaemia, including those with renal impairment.
Pharmacokinetics and optimized dosing of dispersible and non-dispersible levofloxacin formulations in young children
Authors: van der Laan Louvina E , Hesseling Anneke C , Schaaf H Simon , Palmer Megan , Draper Heather R , Wiesner Lubbe , Denti Paolo , Garcia-Prats Anthony J
Published: August 2023

Background

Levofloxacin is used for treatment and prevention of rifampicin-resistant (RR)-TB in children. Recent data showed higher exposures with 100 mg dispersible compared with non-dispersible tablet formulations with potentially important dosing implications in children. We aimed to verify and better characterize this finding.

Methods

We conducted a crossover pharmacokinetic trial in children aged ≤5 years receiving levofloxacin RR-TB preventive therapy. Pharmacokinetic sampling was done after 15–20 mg/kg doses of levofloxacin with 100 mg dispersible and crushed 250 mg non-dispersible levofloxacin formulations. A population pharmacokinetic model was developed.

Results

Twenty-five children were included, median (IQR) weight and age 12.2 (10.7–15.0) kg and 2.56 (1.58–4.03) years, respectively. A two-compartment model with first-order elimination and transit compartment absorption best described levofloxacin pharmacokinetics. Allometric scaling adjusted for body size, and maturation of clearance with age was characterized. Typical clearance in a 12 kg child was estimated at 4.17 L/h. Non-dispersible tablets had 21.5% reduced bioavailability compared with the dispersible formulation, with no significant differences in other absorption parameters.

Dosing simulations showed that current recommended dosing for both formulations result in median exposures below adult-equivalent exposures at a 750 mg daily dose, mainly in children >6 months. Higher levofloxacin doses of 16–30 mg/kg for dispersible and 20–38 mg/kg for crushed non-dispersible tablets may be required in children >6 months.

Conclusions

The dispersible paediatric levofloxacin formulation has improved bioavailability compared with the crushed non-dispersible adult formulation, but exposures remain below those in adults. We propose optimized age- and weight-based dosing for levofloxacin, which require further evaluation.
Treatment outcomes with benzylpenicillin and non-benzylpenicillin antibiotics, and the performance of the penicillin zone-edge test versus molecular detection of blaZ in penicillin-susceptible Staphylococcus aureus (PSSA) bacteraemia
Authors: Mok Hoi Tong , Teng Christine B , Bergin Sarah , Hon Pei Yun , Lye David C , De Partha P , Vasoo Shawn
Published: August 2023

Objectives

The blaZ gene encodes penicillinase, which inactivates penicillin. As there were reports on suboptimal sensitivity for the penicillin zone-edge test, a phenotypic method for blaZ detection, we investigated treatment outcomes in patients with penicillin-susceptible Staphylococcus aureus (PSSA) bacteraemia (phenotypically negative for penicillinase), subjecting isolates to molecular testing for blaZ retrospectively.

Patients and methods

A retrospective cohort study was conducted on 121 patients with a first episode of PSSA bacteraemia from 1 January 2012 to 31 October 2015 at Tan Tock Seng Hospital (TTSH), Singapore. Patients were grouped into IV benzylpenicillin and non-benzylpenicillin groups. The primary outcome was overall treatment failure, defined as either 30 day all-cause mortality and/or 90 day relapse. The penicillin (P10) zone-edge test was repeated on archived PSSA isolates, concurrently with penicillin MIC determination via gradient diffusion and PCR for blaZ.

Results

Among 121 patients, 57 patients (47.1%) received IV benzylpenicillin as the predominant antibiotic. There was no significant difference in overall treatment failure between treatment with the benzylpenicillin [7/57 (12.3%)] versus non-benzylpenicillin groups [12/64 (18.8%)] (P = 0.33) or cloxacillin/cefazolin [6/37 (16.2%)] (P = 0.59). For 112 PSSA isolates available for testing, repeat penicillin zone-edge testing was negative for penicillinase production, corroborating previous results. A single PSSA isolate with a negative penicillin zone-edge test was found to be positive for blaZ.

Conclusions

We found no differences in overall treatment failure between patients with PSSA bacteraemia treated with benzylpenicillin, anti-staphylococcal β-lactams cefazolin/cloxacillin and other antimicrobials, when using the penicillin zone-edge test as the phenotypic method for blaZ screening.
Encapsulation of Pseudomonas aeruginosa strain KBN12 decolourizes and bioremediates brilliant blue dye mediated toxicity in mung bean (Vigna radiata L.)
Authors: Chandwani Sapna , Gajera Aashruti , Riddhi Modi , Gamit Harshida A , Amaresan Natarajan
Published: August 2023

Aim

The aim of this study was to explore the decolourization and bioremediation ability of non-encapsulated and encapsulated Pseudomonas aeruginosa (strain KBN 12) against the azo dye brilliant blue (BB).

Methods and results

Six efficient BB dye-decolourizing bacteria were isolated from textile dye effluent. The most efficient free cells of P. aeruginosa KBN 12 along with the optimized conditions such as carbon source (maltose: 5 g L−1), and nitrogen source (ammonium chloride: 4 g L−1) at pH 6 at 37°C decolourized 72.69% of BB dye aerobically after 9 days of incubation under static conditions. Encapsulated (calcium alginate) P. aeruginosa KBN 12 decolourized 87.67% of BB dye aerobically after 9 days of incubation under the same optimized conditions. Fourier-transform infrared spectroscopy (FTIR) and gas chromatography (GC) analysis of the chemical structure of BB dye after decolourization found changes in functional and chemical groups. Phytotoxicity and soil respiration enzyme assays revealed that the decolourized dye or dye products were less toxic than the pure BB dye.

Conclusion

The encapsulation of P. aeruginosa KBN 12 proved to be an effective method for BB dye decolourization or remediation.
Main Pathogens Causing Infections and Antibiotic Resistance Profile in Major Burns in Brazil Comparing Two Periods: 2015/2016 and 2019/2020
Authors: Rissetto Nicoli Gueno , de Oliveira Julia Werner , Nisihara Renato
Published: August 2023

Sepsis is the main cause of death in major burns. In this retrospective study conducted in a reference hospital in Brazil, the main agents causing infection and the resistance profile to antibiotics were identified. In addition, the epidemiological profile, length of hospital stay, type of burn, and total body surface area (TBSA) in major burns were collected from medical records, comparing the years 2015/2016 and 2019/2020. In both time periods, there was a predominance of males with a mean age of 43 years. Burns with less than 30% of TBSA predominated. There was a significant increase in positive cultures (P = .00026), from 58.7% to 80%, and an increase in skin punch culture collection from 25.6% to 43.9% in the years 2019/2020. The predominant etiological agent was Pseudomonas aeruginosa, followed by Acinetobacter baumannii resistant to carbapenems, and Staphylococcus aureus in both evaluated periods. The percentage of deaths was higher in 2019/2020 (26.2% vs. 14.6%; P = .026). The length of hospital stay was shorter in the latter period (P = .0081). Sepsis was the cause of death in 81.2% of cases in 2015/2020 and 78.3% in 2019/2020. Among the deaths, P. aeruginosa was the main agent identified. There was no change in the main pathogens and bacterial antibiotic resistance between the 2 time periods.
Global Burn Prevention: Ukraine
Authors: Dabek Robert J , Decik Myroslava , Driscoll Daniel N , Fuzaylov Gennadiy
Published: August 2023

Burn injury accounts for a large proportion of surgically treatable disease. It is estimated that over 180 000 flame burn deaths occur annually across the globe, with roughly 95% occurring in low- or middle-income countries (LMIC). Within these countries, children account for a disproportionately high number of burn injuries. As such, the WHO has identified burn prevention as a topic of interest, with an increased need in LMIC. Here, we describe the creation and implementation of a burn prevention program in Ukraine. We instituted a 5-step burn prevention initiative consisting of; data gathering, program design, implementation, outcome evaluation, program maintenance, and expansion. The burn prevention initiative has been adopted nationally leading to policy change. Active education and an information campaign were used to target pediatric scald injuries and improve first-aid care. The authors have successfully implemented a targeted multifaceted, national, burn prevention program within Ukraine. The described approach may be used as a guide and adapted to create similar prevention programs within other countries or regions. At this time, the Russo-Ukrainian War has caused an abrupt hold on our role in the prevention program and left the healthcare system in havoc. Moving forward, our team is prepared to re-evaluate the impact of the war on social life and the medical system and aid in redesigning the prevention program when appropriate.
Variation and taphonomic implications of composition in modern and fossil malacostracan cuticles (Decapoda: Malacostraca)
Authors: Plotnick Roy E , McCarroll Steve
Published: August 2023

The crustacean fossil record is dominated by mineralized forms. These fossils, in turn, are mainly decapod chelae or parts of chelae. Studies of the composition of modern crustacean cuticles have likewise been dominated by decapods. We summarize major element chemical analyses (Ca, Mg, Na, P) using SEM-EDS of modern crustacean exoskeletons from Bermuda, Georgia (USA), and Lake Tanganyika (Burundi). Although most specimens were brachyuran and anomuran crabs, material also included examples of stomatopods, isopods, caridean shrimps, and achelatans. Transects were made of sections of the carapace, palm of the propodus, and tips of either the dactyl or propodus. Multiple transects were made of the Bermudan land crab Gecarcinus lateralis. Additional studies were made of brachyuran fossil crabs from Texas and South Dakota and of brachyuran crabs having undergone ten months of burial in marine sediments. Overall, the tips of the chela were the thickest and most heavily mineralized regions and had the lowest phosphorus content. In nearly all cases, they also had the lowest ratios of Mg to Ca. One notable exception was Gecarcinus, where the denticles had elevated Mg:Ca values. Anomura and Brachyura had markedly different composition from Stomatopoda and possibly Isopoda, suggestive of independent evolutionary origins of biomineralization within the Crustacea. There were also significant differences in composition between brachyuran crabs from Georgia and Bermuda, perhaps due to differences in water temperature. The fossil crustaceans had much more phosphate in their cuticles, likely as a result of diagenetic processes. Composition should be a first order control on the fossil record of crustaceans. Future studies need to expand the taxonomic and geographic ranges of material examined, determine whether there are environmental controls on composition, and explore the relevant diagenetic processes.
Exploring a Right to Submit Environmental Information Under International Environmental Law
Authors: Whittaker Sean
Published: August 2023

Discussions on how the public engages with environmental information within environmental governance matters are primarily centred around the supply of environmental information to the public by the state. However, this focus downplays the importance of environmental information held by members of the public and the difficulties that individuals can experience when submitting such information to the state outside formal environmental decision-making procedures. This article examines the benefits of guaranteeing a general right to submit environmental information before considering the extent to which environmental law, specifically the Aarhus Convention, supports the creation of such a right. The article then concludes by constructing a hypothetical regime to implement the proposed right, identifying how the proposed right can be balanced against the needs of the state and points of divergence between this hypothetical regime and the current environmental information regime set out in the Aarhus Convention.
Dynamical characterization of the 6/1 mean motion resonance between Quaoar’s ring and Weywot
Authors: Rodríguez Adrián , Morgado B E , Callegari Jr. N
Published: August 2023

Recently, it has been reported the discovery of a dense ring around the trans-Neptunian object 50000 Quaoar. The ring particles seem to be very close to the 6/1 mean motion resonance with Weywot, the only known satellite in the system. In this work, we investigate the dynamical environment in the close vicinity of the 6/1 orbital resonance in the context of the restricted three-body problem. We aim to analyse whether, in view of observational constraints, the ring could be effectively evolving in resonant motion with the satellite. Through the technique of dynamical maps we identify and characterize the 6/1 mean motion resonance, finding that the main location of the resonance deviates by only 29 km from the central part of the ring. This difference lies within the 3σ confidence level, considering the uncertainties in the observational parameters. We also show that the Weywot’s eccentricity plays a significant role in the dynamical structure of the 6/1 resonance. The results show that the resonance width is smaller than the estimated ring’s width. Under assumption of a ring with eccentricity smaller than 0.05, clumping of test particles appears at the position of the different resonant multiplets, considering the nominal value of Weywot’s eccentricity. This is in agreement with observations, which indicate that the estimated resonance width (≤10 km) is comparable with the narrow and dense arc of material within Quaoar’s ring. Our results may be an indicative that the 6/1 resonance plays a key role in confining the arc ring.
Study of structural parameters and systemic proper motion of Sextans dwarf spheroidal galaxy with Subaru Hyper Suprime-Cam data
Authors: Tokiwa Akira , Takada Masahiro , Qiu Tian , Yasuda Naoki , Komiyama Yutaka , Chiba Masashi , Hayashi Kohei
Published: August 2023

We use the Subaru Hyper Suprime-Cam (HSC) data to study structural parameters and systemic proper motion of the Sextans dwarf spheroidal galaxy at the heliocentric distance of 86 kpc, which is one of the most important targets for studies of dark matter nature and galaxy formation physics. Thanks to the superb image quality and wide area coverage, the HSC data enable a secure selection of member star candidates based on the colour–magnitude cut, yielding about 10 000 member candidates at magnitudes down to i ∼ 24. We use a likelihood analysis of the two-dimensional distribution of stars to estimate the structural parameters of Sextans taking into account the contamination of foreground halo stars in the Milky Way, and find that the member star distribution is well fitted by an elliptical King profile with ellipticity ϵ ≃ 0.25 and the core and tidal radii of Rc = (368.4 ± 8.5) pc and Rt = (2.54 ± 0.046) kpc, respectively. Then using the two HSC data sets of 2.66 yr time baseline on average, we find the systemic proper motions of Sextans to be (μα, μδ) = (−0.448 ± 0.075, 0.058 ± 0.078) mas yr−1, which are consistent with some of the previous works using the Gaia data of relatively bright member stars in Sextans. Thus, our results give a demonstration that ground-based, large-aperture telescope data that cover a wide solid angle of the sky and have a long time baseline, such as the upcoming data from Legacy Survey of Space and Time (LSST), can be used to study systemic proper motions of dwarf galaxies.
Modelling the expected very high energy γ-ray emission from accreting neutron stars in X-ray binaries
Authors: Ducci L , Romano P , Vercellone S , Santangelo A
Published: August 2023

The detection of γ-ray emission from accreting pulsars in X-ray binaries (XRBs) has long been sought after. For some high-mass X-ray binaries (HMXBs), marginal detections have recently been reported. Regardless of whether these will be confirmed or not, future telescopes operating in the γ-ray band could offer the sensitivity needed to achieve solid detections and possibly spectra. In view of future observational advances, we explored the expected emission above 10 GeV from XRBs, based on the Cheng & Ruderman model, where γ-ray photons are produced by the decay of π0 originated by protons accelerated in the magnetosphere of an accreting pulsar fed by an accretion disc. We improved this model by considering, through Monte Carlo simulations, the development of cascades inside of and outside the accretion disc, taking into account pair and photon production processes that involve interaction with nuclei, X-ray photons from the accretion disc, and the magnetic field. We produced grids of solutions for different input parameter values of the X-ray luminosity (Lx), magnetic field strength (B), and for different properties of the region where acceleration occurs. We found that the γ-ray luminosity spans more than five orders of magnitude, with a maximum of ∼1035 erg s−1. The γ-ray spectra show a large variety of shapes: some have most of the emission below ∼100 GeV, others are harder (emission up to 10–100 TeV). We compared our results with Fermi/LAT and VERITAS detections and upper limits of two HMXBs: A0535+26 and GRO J1008−57. More consequential comparisons will be possible when more sensitive instruments will be operational in the coming years.
On the conditions for warping and breaking protoplanetary discs
Authors: Young Alison K , Stevenson Struan , Nixon C J , Rice Ken
Published: August 2023

Recent observations demonstrate that misalignments and other out-of-plane structures are common in protoplanetary discs. Many of these have been linked to a central host binary with an orbit that is inclined with respect to the disc. We present simulations of misaligned circumbinary discs with a range of parameters to gain a better understanding of the link between those parameters and the disc morphology in the wave-like regime of warp propagation that is appropriate to protoplanetary discs. The simulations confirm that disc tearing is possible in protoplanetary discs as long as the mass ratio, μ, and disc–binary inclination angle, i, are not too small. For the simulations presented here, this corresponds to μ > 0.1 and i ≳ 40°. For highly eccentric binaries, tearing can occur for discs with smaller misalignment. Existing theoretical predictions provide an estimate of the radial extent of the disc in which we can expect breaking to occur. However, there does not seem to be a simple relationship between the disc properties and the radius within the circumbinary disc at which the breaks appear, and furthermore the radius at which the disc breaks can change as a function of time in each case. We discuss the implications of our results for interpreting observations and suggest some considerations for modelling misaligned discs in the future.
Characterizing fragmentation and sub-Jovian clump properties in magnetized young protoplanetary discs
Authors: Kubli Noah , Mayer Lucio , Deng Hongping
Published: August 2023

We study the initial development, structure, and evolution of protoplanetary clumps formed in three-dimensional resistive magnetohydrodynamic (MHD) simulations of self-gravitating discs. The magnetic field grows by means of the recently identified gravitational instability dynamo. Clumps are identified and their evolution is tracked finely both backward and forward in time. Their properties and evolutionary path is compared with clumps in companion simulations without magnetic fields. We find that magnetic and rotational energy are important in the clumps’ outer regions, while in the cores, despite appreciable magnetic field amplification, thermal pressure is most important in counteracting gravity. Turbulent kinetic energy is of a smaller scale than magnetic energy in the clumps. Compared with non-magnetized clumps, rotation is less prominent, which results in lower angular momentum in much better agreement with observations. In order to understand the very low sub-Jovian masses of clumps forming in MHD simulations, we revisit the perturbation theory of magnetized sheets finding support for a previously proposed magnetic destabilization in low-shear regions. This can help explaining why fragmentation ensues on a scale more than an order of magnitude smaller than that of the Toomre mass. The smaller fragmentation scale and the high magnetic pressure in clumps’ envelopes explain why clumps in magnetized discs are typically in the super-Earth to Neptune mass regime rather than super-Jupiter as in conventional disc instability. Our findings put forward a viable alternative to core accretion to explain widespread formation of intermediate-mass planets.
Critical evaluation of cell lysis methods for metallodrug studies in cancer cells
Authors: Riisom Mie , Jamieson Stephen M F , Hartinger Christian G
Published: August 2023

Intracellular accumulation studies are a key step in metallodrug development but often variable results are obtained. Therefore, we aimed here to investigate different protocols for efficient and reproducible lysis of cancer cells in terms of protein content in lysates and in cell uptake studies of the Ru anticancer complex [chlorido(8-oxyquinolinato)(η6-p-cymene)ruthenium(II)] ([Ru(cym)(HQ)Cl]). The physical lysis methods osmosis and sonication were chosen for comparison with chemical lysis with the radioimmunoprecipitation assay (RIPA) buffer. Based on the protein content and the total Ru accumulated in the lysates, the latter determined using inductively coupled plasma–mass spectrometry, RIPA buffer was the most efficient lysis method. Measurements of plastic adsorption blanks revealed that the higher Ru content determined in the RIPA buffer lysis samples may be due a higher amount of Ru extracted from the plastic incubation plates compared with osmosis and sonication. Overall, we found that the choice of lysis method needs to be matched to the information sought and we suggest the least disruptive osmosis method might be the best choice for labile drug–biomolecule adducts. Minimal differences were found for experiments aimed at measuring the overall cell uptake of the Ru complex.
A neuron-immune circuit regulates neurodegeneration in the hindbrain and spinal cord of Arf1-ablated mice
Authors: Wang Guohao , Jin Shuhan , Liu Jiaqi , Li Xu , Dai Peng , Wang Yuetong , Hou Steven X
Published: August 2023

Neuroimmune connections have been revealed to play a central role in neurodegenerative diseases (NDs). However, the mechanisms that link the central nervous system (CNS) and peripheral immune cells are still mostly unknown. We recently found that specific ablation of the Arf1 gene in hindbrain and spinal cord neurons promoted NDs through activating the NLRP3 inflammasome in microglia via peroxided lipids and adenosine triphosphate (ATP) releasing. Here, we demonstrate that IL-1β with elevated chemokines in the neuronal Arf1-ablated mouse hindbrain and spinal cord recruited and activated γδ T cells in meninges. The activated γδ T cells then secreted IFN-γ that entered into parenchyma to activate the microglia-A1 astrocyte-C3-neuronal C3aR neurotoxic pathway. Remarkably, the neurodegenerative phenotypes of the neuronal Arf1-ablated mice were strongly ameliorated by IFN-γ or C3 knockout. Finally, we show that the Arf1-reduction-induced neuroimmune-IFN-γ-gliosis pathway exists in human NDs, particularly in amyotrophic lateral sclerosis and multiple sclerosis. Together, our results uncover a previously unknown mechanism that links the CNS and peripheral immune cells to promote neurodegeneration.
Health Equity and Antibiotic Prescribing in the United States: A Systematic Scoping Review
Authors: Kim Christine , Kabbani Sarah , Dube William C , Neuhauser Melinda , Tsay Sharon , Hersh Adam , Marcelin Jasmine R , Hicks Lauri A
Published: August 2023

We performed a scoping review of articles published from 1 January 2000 to 4 January 2022 to characterize inequities in antibiotic prescribing and use across healthcare settings in the United States to inform antibiotic stewardship interventions and research. We included 34 observational studies, 21 cross-sectional survey studies, 4 intervention studies, and 2 systematic reviews. Most studies (55 of 61 [90%]) described the outpatient setting, 3 articles were from dentistry, 2 were from long-term care, and 1 was from acute care. Differences in antibiotic prescribing were found by patient's race and ethnicity, sex, age, socioeconomic factors, geography, clinician's age and specialty, and healthcare setting, with an emphasis on outpatient settings. Few studies assessed stewardship interventions. Clinicians, antibiotic stewardship experts, and health systems should be aware that prescribing behavior varies according to both clinician- and patient-level markers. Prescribing differences likely represent structural inequities; however, no studies reported underlying drivers of inequities in antibiotic prescribing.