bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Minimally Invasive Monitoring Methods and Live Trapping in Mammals
Authors: Miranda Paez Andrea , Sundaram Mekala , Willoughby Janna R.
Published: December 2021

The conservation and management of wildlife requires the accurate assessment of wildlife population sizes. However, there is a lack of synthesis of research that compares methods used to estimate population size in the wild. Using a meta-analysis approach, we compared the number of detected individuals in a study made using live trapping and less invasive approaches, such as camera trapping and genetic identification. We scanned 668 papers related to these methods and identified data for 44 populations (all focused on mammals) wherein at least two methods (live trapping, camera trapping, genetic identification) were used. We used these data to quantify the difference in number of individuals detected using trapping and less invasive methods using a regression and used the residuals from each regression to evaluate potential drivers of these trends. We found that both trapping and less invasive methods (camera traps and genetic analyses) produced similar estimates overall, but less invasive methods tended to detect more individuals compared to trapping efforts (mean = 3.17 more individuals). We also found that the method by which camera data are analyzed can significantly alter estimates of population size, such that the inclusion of spatial information was related to larger population size estimates. Finally, we compared counts of individuals made using camera traps and genetic data and found that estimates were similar but that genetic approaches identified more individuals on average (mean = 9.07 individuals). Overall, our data suggest that all of the methods used in the studies we reviewed detected similar numbers of individuals. As live trapping can be more costly than less invasive methods and can pose more risk to animal well-fare, we suggest minimally invasive methods are preferable for population monitoring when less-invasive methods can be deployed efficiently.
NGS Evaluation of a Bernese Cohort of Unexplained Erythrocytosis Patients
Authors: Jalowiec Katarzyna Aleksandra , Vrotniakaite-Bajerciene Kristina , Capraru Annina , Wojtovicova Tatiana , Joncourt Raphael , Rovó Alicia , Porret Naomi A.
Published: December 2021

(1) Background: Clinical and molecular data on patients with unexplained erythrocyto-sis is sparse. We aimed to analyze the clinical and molecular features of patients with congenital erythrocytosis in our tertiary reference center. (2) Methods: In 34 patients with unexplained erythrocytosis, a 13-gene Next-Generation Sequencing erythrocytosis panel developed at our center was conducted. (3) Results: In 6/34 (18%) patients, eight different heterozygous gene variants were found. These patients were, therefore, diagnosed with congenital erythrocytosis. Two patients had two different gene variants each. All variants were characterized as variants of unknown significance as they had not previously been described in the literature. The rest of the patients (28/34, 82%) had no detected gene variants. (4) Conclusions: Our experience shows that the NGS panel can be helpful in determining the reasons for persistent, unexplained erythrocytosis. In our cohort of patients with erythrocytosis, we identified some, thus far unknown, gene variants which may explain the clinical picture. However, further investigations are needed to determine the relationship between the molecular findings and the phenotype.
Genome-Wide Analysis of Gene Families of Pattern Recognition Receptors in Fig Wasps (Hymenoptera, Chalcidoidea)
Authors: Hou Hong-Xia , Huang Da-Wei , Xin Zhao-Zhe , Xiao Jin-Hua
Published: December 2021

Pattern recognition receptors (PRRs) play important roles in detecting pathogens and initiating the innate immune response. Different evolutionary histories of pollinators and non-pollinators may result in different immune recognition systems. A previous study had reported that there were significant differences in peptidoglycan recognition proteins (PGRPs) between pollinators and non-pollinators in gene number and lineage of specific genes. In this study, based on the genomic data of 12 fig wasp species, with seven pollinators and five non-pollinators, we investigated the evolution patterns of PRRs, such as Gram-negative bacteria-binding proteins (GNBPs), C-type lectins (CTLs), scavenger receptors class B (SCRBs), fibrinogen-related proteins (FREPs), galectins, and thioester-containing proteins (TEPs). Our results showed that pollinators had no GNBP, but non-pollinators all had two gene members, which were clustered into two different clades in the phylogenetic tree, with each clade having specific domain and motif characteristics. The analysis of CTL and SCRB gene families also showed that there were lineage-specific genes and specific expansion in non-pollinators. Our results showed that there were significant differences in immune recognition between pollinators and non-pollinators, and we concluded that they had undergone flexible adaptive evolution in different environments. Our study can provide more molecular evidence for future functional studies on the immune system of fig wasps.
Bibliometric Analysis of Remote Sensing of Inland Waters Publications from 1985 to 2020
Authors: Ogashawara Igor
Published: December 2021

Over the past few decades, there has been a significant increase in the number of studies about the use of remote sensing techniques for the monitoring of inland waters. Since these aquatic environments have multiple uses for mankind, monitoring them is essential for the environment, society, and the economy. The use of Earth Observations data for the monitoring of inland waters is important for water quantity and quality management. Because of this, the goal of the present study is to systematically analyze and visualize the evolution of publications in this field. This study will not focus on algorithm comparisons or descriptions; instead, it will use a co-citation analysis to map the relationship among publications. Using the Web of Science database, publications related to the terms, “remote sensing” + “inland waters”, were analyzed using the entire database. The bibliometric analysis showed how research topics evolved from measuring optical properties and understanding their signal to the development of remote sensing algorithms and their applications to satellite imagery. This study provides the historical development of the scientific publications in this field and points out what could be the direction for future research.
Exploring the Competencies of Japanese Expert Nurse Practitioners: A Thematic Analysis
Authors: Igarashi Mari , Ohta Ryuichi , Kurita Yasuo , Nakata Akinori , Yamazaki Tsutomu , Gomi Harumi
Published: December 2021

Nurse practitioners (NPs) provide medical care equivalent to that of physicians and facilitate access to healthcare. Although Japan’s first NP graduated in 2010, how Japanese expert NPs work effectively in clinical contexts is yet to be investigated. We aimed to identify the competencies that make expert NPs in Japan effective. Twelve Japanese expert NPs were purposely selected. The average age of the participants was 44.8 years, average NP experience was 7.5 years, and eight participants were women. Semi-structured interviews were conducted online from March to May 2021. Interviews were recorded, transcribed verbatim, and analyzed using thematic analysis. Thematic analysis revealed five themes: working in physicians’ contexts, interprofessional collaboration, involvement in nurses’ work, contribution to healthcare, and personal qualities for effective working. Japanese expert NPs can function effectively in clinical settings by flexibly and humbly collaborating with other medical professionals who have autonomous positions. They can improve the quality of healthcare by proposing practical solutions to problems faced by patients and medical organizations. These explored competencies can be applied to other aging and more complex societal contexts, and in updating the required competencies of Japanese NPs.
Influence of Attachment Anxiety on the Relationship between Loneliness and Depression among Long-Term Care Residents
Authors: Arunrasameesopa Suthikarn , Wongpakaran Nahathai , Wongpakaran Tinakon
Published: December 2021

Background: Attachment styles influence an individual’s ability to maintain relationships throughout their lifespan. Insecure attachment has been associated with many aspects of mental health. The study aimed to explore the influence of attachment on loneliness and depression among old-age residents in long-term care facilities. Methods: A survey was conducted among residents from long term care facilities (LTC) in Thailand during the COVID-19 pandemic; 221 older people participated in the study. The mean aged was 73.60 ± 7.45 years old, and 57.5% female. All participants completed the Geriatric Depression Scale, the six-item revised version of the University of California Los Angeles Loneliness Scale, and the Revised Experience of Close Relationships questionnaire to measure depressive symptoms, loneliness, and attachment style. Hierarchical multiple regression analysis was performed to find the predictors for depressive symptoms. Results: In all, 40.7% of residents in LTC facilities experienced depression. Male sex, education, loneliness, and attachment anxiety predicted the increased level of depressive symptoms. Conclusion: Loneliness was confirmed to be significantly associated with depressive symptoms, and attachment anxiety was found to be a strong predicting factor of depressive symptoms. Intervention and research regarding reducing loneliness and attachment anxiety should be promoted in LTC settings.
In Vitro and In Vivo Antifungal Activities of Nine Commercial Essential Oils against Brown Rot in Apples
Authors: El Khetabi Assia , Ezrari Said , El Ghadraoui Lahsen , Tahiri Abdessalem , Ait Haddou Lhoussain , Belabess Zineb , Merah Othmane , Lahlali Rachid
Published: December 2021

After harvest, numerous plant pathogenic fungi can infect fresh fruits during transit and storage. Although synthetic fungicides are often used to manage postharvest fruit diseases, their application may lead to problems such as the development of fungicide resistance and residues on fruits. In the present study, the antifungal potential of nine commercial essential oils (EOs) extracted from Eucalyptus radiata ssp. radiata, Mentha pulegium, Rosmarinus officinalis, Origanum compactum, Lavandula angustifolia, Syzygium aromaticum, Thymus vulgaris, Citrus aurantium, and Citrus sinensis were tested against the apple brown rot fungi Monilinia laxa and Monilinia fructigena at different concentrations in vitro (against mycelial growth and spore germination) and in vivo (on detached apple fruit and in semi-commercial postharvest conditions). In addition, fruit quality parameters were evaluated and the composition of the EOs was characterized by Fourier transform infrared (FT-IR) spectroscopy. In vitro results showed significant antifungal activity of all tested EOs on both fungal species. EOs from S. aromaticum were the most effective, whereby inhibition percentages ranged from 64.0 to 94.7% against M. laxa and from 63.9 to 94.4% against M. fructigena for the concentrations 12.5 and 100 µL/mL, respectively, with an EC50 of 6.74 µL/mL for M. laxa and 10.1 µL/mL for M. fructigena. The higher concentrations tested of S. aromaticum, T. vulgaris, C. aurantium, and C. sinensis EOs significantly reduced spore germination, brown rot incidence, and lesion diameter. Evaluation of the treatments during storage for 20 days at 4 °C on apple fruit quality parameters demonstrated the preservation of the fruit quality characteristics studied (weight loss, total soluble solids, titratable acidity, firmness, and maturity index). FT-IR spectra obtained from all tested EO samples presented characteristic peaks and a high diversity of functional groups such as O–H groups, C–H bonds, and C–C stretching. The EOs examined here may have the potential for controlling postharvest fungal diseases of fruit such as brown rot.
Financial Support Program for Small Farmers, and Its Impact on Local Food Security. Evidence from Indonesia
Authors: Purnawan Endar , Brunori Gianluca , Prosperi Paolo
Published: December 2021

This paper addresses the implementation of the Kawasan Mandiri Pangan (KMP) program, a microfinance program for farmer groups, assessing whether the program affects farmers’ decisions concerning production, marketing, and consumption or not, and its impacts on household food security along three dimensions: food availability, food access, and food utilization. Based on a qualitative and theory of change mixed-methods analysis, which uses interviews and focus group discussions (FGDs), this research sheds light on the program’s success among two groups of farmers. Both groups experienced improved productivity and increased food availability, but only one group sustained the program. The results indicate that the program has not affected the commercialization of any particular crop, where the crop’s best selling price, relationships, and commitments are factors that affect the farmers’ marketing decisions. Other findings show how food access at the household level increased when the crop’s selling price was reasonable, while food utilization was influenced predominantly by local wisdom. Taken together, the research findings highlight the importance of the capability of the management, the commitment of the members, and the supervision of the agricultural extension agents. There is a need for a locally owned enterprise to absorb agricultural products and maintain the selling price of crops, which is the primary driver of food accessibility and utilization at the household level.
Techniques for Reducing the Abundance of Spring–Summer Flush Shoots in Southern Spanish Orange Orchards
Authors: Arenas-Arenas Francisco J. , Arjona-López Juan M. , Romero-Rodríguez Estefanía , Calero-Velázquez Rocío , Hervalejo Aurea
Published: December 2021

The Mediterranean Basin is the second highest citrus growing region in the world behind China. Citrus trees are known to produce several flush shoots per year, particularly during the spring–summer season. Farmers endeavor to reduce the growth of summer shoots by means of hand pruning, especially those located at the top of the tree, as most of these shoots become vigorous, nutrient consuming, non-productive, and attractive to several pests. Furthermore, hand pruning substantially increases the costs of citrus orchards production. This research was therefore intended to study new different treatments to control spring–summer flush shoots and thus reduce growers’ investments in citrus production. Six different treatments were applied in two experimental and high density orange orchards over two consecutive years: (1) control; (2) topping (mechanical pruning); (3) dichlorprop-p; (4) triclopyr; (5) topping + dichlorprop-p; and (6) topping + triclopyr. The treatment of dichlorprop-p alone reduced the number of summer young shoots in both years. Moreover, these applications did not negatively affect yield or fruit quality. These mechanical methodologies help citrus growers manage the density of flush shoots and reduce hand labor costs in citrus orchards.
Dietary Behaviour and Nutrition in Patients with COPD Treated with Long-Term Oxygen Therapy
Authors: Mekal Dominika , Czerw Aleksandra , Deptala Andrzej
Published: December 2021

Background: It is the first study in Poland and one of the first in the world to assess the nutrition of patients with chronic obstructive pulmonary disease (COPD) treated with long-term oxygen therapy (LTOT). Methods: The study group consisted of 110 COPD patients treated with LTOT. Anthropometric measurements and spirometry were performed. The diet of patients was assessed using a 3-day nutrition diary. Results: When assessing the degree of airflow obstruction (FEV1% N) depending on the BMI in patients treated with LTOT, a statistically significant correlation was demonstrated between the BMI and the value of the FEV% N parameter (p = 0.0093). Patients with COPD with a BMI >30 had statistically significantly higher values of FEV1% N than patients with a BMI in the range of 20–24.9 (p = 0.0278). Intake of calcium, vitamins A, C, D, E and folates was lower than the recommended daily intake in more than 95% of COPD patients. Conclusions: The diet of COPD patients treated with long-term oxygen therapy was improperly balanced, with deficiencies of important nutrients. Airflow obstruction in the respiratory tract was significantly smaller in obese patients, and greater in patients with diagnosed malnutrition.
Reconstructing Missing and Anomalous Data Collected from High-Frequency In-Situ Sensors in Fresh Waters
Authors: Kermorvant Claire , Liquet Benoit , Litt Guy , Jones Jeremy B. , Mengersen Kerrie , Peterson Erin E. , Hyndman Rob J. , Leigh Catherine
Published: December 2021

In situ sensors that collect high-frequency data are used increasingly to monitor aquatic environments. These sensors are prone to technical errors, resulting in unrecorded observations and/or anomalous values that are subsequently removed and create gaps in time series data. We present a framework based on generalized additive and auto-regressive models to recover these missing data. To mimic sporadically missing (i) single observations and (ii) periods of contiguous observations, we randomly removed (i) point data and (ii) day- and week-long sequences of data from a two-year time series of nitrate concentration data collected from Arikaree River, USA, where synoptically collected water temperature, turbidity, conductance, elevation, and dissolved oxygen data were available. In 72% of cases with missing point data, predicted values were within the sensor precision interval of the original value, although predictive ability declined when sequences of missing data occurred. Precision also depended on the availability of other water quality covariates. When covariates were available, even a sudden, event-based peak in nitrate concentration was reconstructed well. By providing a promising method for accurate prediction of missing data, the utility and confidence in summary statistics and statistical trends will increase, thereby assisting the effective monitoring and management of fresh waters and other at-risk ecosystems.
Impact of the Cartographer’s Position and Topographic Accessibility on the Accuracy of Historical Land Use Information: Case of the Second Military Survey Maps of the Habsburg Empire
Authors: Ostafin Krzysztof , Pietrzak Małgorzata , Kaim Dominik
Published: December 2021

Historical maps are critical for long-term land use reconstructions; however, quantifying the uncertainty involved in comparing historical maps with recent data remains a considerable challenge. To date, many works have focused on the technical aspects of comparing historical and contemporary materials, but the potential sources of uncertainty inherent in historical data remain poorly understood. In this paper, we analyze the impacts of the topographic accessibility and cartographer’s field position on the content quality of historical Austrian second military survey maps by referring to independent census data. Our results show that the topographic accessibility and visibility from the cartographer’s surveying table points had very little impact on the map content quality and that the surveying table point locations were uniformly distributed throughout the area, regardless of the landscape conditions. These findings demonstrate that the second military survey maps can be seen as valuable and consistent historical data sources, making them especially useful for long-term land use research in Central Europe.
A Continuous Taxi Pickup Path Recommendation under The Carbon Neutrality Context
Authors: Chang Mengmeng , Chi Yuanying , Ding Zhiming , Tian Jing , Zheng Yuhao
Published: December 2021

In the context of the carbon neutrality target, carbon reduction in the daily operation of the transportation system is more important than that in productive activities. There are few travel services that can quantify low-carbon travel, with a lack of effective low-carbon travel tools to guide transportation behavior. On-demand access to taxi services can effectively reduce the additional carbon emissions caused by cruising, which in turn increases efficiency in urban mobility with a reduced taxi fleet scale. For individual taxis, they lack macroscopic horizon in their choice of passenger pickup paths. The selected travel path based on personal operational experience or real-time location is limited by local optimization when making path decisions. In this work, we proposed a macro-path recommendation method to assist the taxi pickup path selection to accelerate the transformation of the taxi system towards low-carbon sharing. First, an adaptive learning spatiotemporal neural network was used to predict the coarse-grained distribution of potential trips. Next, the trajectory sharing graph was constructed based on the potential trips distribution to reallocate the taxi orders for the continuous pickup path optimization. As a result, the continuous pickup path balanced the relation between travel demands and taxi supply, improving the economic and environmental benefits of taxi operation and contributing to the goal of carbon neutrality. We conducted experiments on the Chengdu city ride-hailing dataset. Compared with the current status of taxi operations, the solution shows improvements in both the scale of taxi services and order gain.
Validation of SV2A-Targeted PET Imaging for Noninvasive Assessment of Neuroendocrine Differentiation in Prostate Cancer
Authors: Guan Bing , Zhou Ning , Wu Cheng-Yang , Li Songye , Chen Yu-An , Debnath Sashi , Hofstad Mia , Ma Shihong , Raj Ganesh V. , He Dalin , Hsieh Jer-Tsong , Huang Yiyun , Hao Guiyang , Sun Xiankai
Published: December 2021

Neuroendocrine prostate cancer (NEPC) is an aggressive and lethal variant of prostate cancer (PCa), and it remains a diagnostic challenge. Herein we report our findings of using synaptic vesicle glycoprotein 2 isoform A (SV2A) as a promising marker for positron emission tomography (PET) imaging of neuroendocrine differentiation (NED). The bioinformatic analyses revealed an amplified SV2A gene expression in clinical samples of NEPC versus castration-resistant PCa with adenocarcinoma characteristics (CRPC-Adeno). Importantly, significantly upregulated SV2A protein levels were found in both NEPC cell lines and tumor tissues. PET imaging studies were carried out in NEPC xenograft models with 18F-SynVesT-1. Although 18F-SynVesT-1 is not a cancer imaging agent, it showed a significant uptake level in the SV2A+ tumor (NCI-H660: 0.70 ± 0.14 %ID/g at 50–60 min p.i.). The SV2A blockade resulted in a significant reduction of tumor uptake (0.25 ± 0.03 %ID/g, p = 0.025), indicating the desired SV2A imaging specificity. Moreover, the comparative PET imaging study showed that the DU145 tumors could be clearly visualized by 18F-SynVesT-1 but not 68Ga-PSMA-11 nor 68Ga-DOTATATE, further validating the role of SV2A-targeted imaging for noninvasive assessment of NED in PCa. In conclusion, we demonstrated that SV2A, highly expressed in NEPC, can serve as a promising target for noninvasive imaging evaluation of NED.
Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production
Authors: Zhou Xin , Li Junhong , Giannopoulos Antonios , Kingham Paul J. , Backman Ludvig J.
Published: December 2021

It is known that mechanical loading of muscles increases the strength of healing tendon tissue, but the mechanism involved remains elusive. We hypothesized that the secretome from myoblasts in co-culture with tenocytes affects tenocyte migration, cell phenotype, and collagen (Col) production and that the effect is dependent on different types of mechanical loading of myoblasts. To test this, we used an in vitro indirect transwell co-culture system. Myoblasts were mechanically loaded using the FlexCell® Tension system. Tenocyte cell migration, proliferation, apoptosis, collagen production, and several tenocyte markers were measured. The secretome from myoblasts decreased the Col I/III ratio and increased the expression of tenocyte specific markers as compared with tenocytes cultured alone. The secretome from statically loaded myoblasts significantly enhanced tenocyte migration and Col I/III ratio as compared with dynamic loading and controls. In addition, the secretome from statically loaded myoblasts induced tenocytes towards a myofibroblast-like phenotype. Taken together, these results demonstrate that the secretome from statically loaded myoblasts has a profound influence on tenocytes, affecting parameters that are related to the tendon healing process.
Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis
Authors: Hsu Shen-Hsing , Chou Li-Fang , Hong Chung-Hung , Chang Ming-Yang , Tsai Chung-Ying , Tian Ya-Chung , Yang Huang-Yu , Yang Chih-Wei
Published: December 2021

Approximately 1 million cases of leptospirosis, an emerging infectious zoonotic disease, are reported each year. Pathogenic Leptospira species express leucine-rich repeat (LRR) proteins that are rarely expressed in non-pathogenic Leptospira species. The LRR domain-containing protein family is vital for the virulence of pathogenic Leptospira species. In this study, the biological mechanisms of an essential LRR domain protein from pathogenic Leptospira were examined. The effects of Leptospira and recombinant LRR20 (rLRR20) on the expression levels of factors involved in signal transduction were examined using microarray, quantitative real-time polymerase chain reaction, and western blotting. The secreted biomarkers were measured using an enzyme-linked immunosorbent assay. rLRR20 colocalized with E-cadherin on the cell surface and activated the downstream transcription factor β-catenin, which subsequently promoted the expression of MMP7, a kidney injury biomarker. Additionally, MMP7 inhibitors were used to demonstrate that the secreted MMP7 degrades surface E-cadherin. This feedback inhibition mechanism downregulated surface E-cadherin expression and inhibited the colonization of Leptospira. The degradation of surface E-cadherin activated the NF-κB signal transduction pathway. Leptospirosis-associated acute kidney injury is associated with the secretion of NGAL, a downstream upregulated biomarker of the NF-κB signal transduction pathway. A working model was proposed to illustrate the crosstalk between E-cadherin/β-catenin and NF-κB signal transduction pathways during Leptospira infection. Thus, rLRR20 of Leptospira induces kidney injury in host cells and inhibits the adhesion and invasion of Leptospira through the upregulation of MMP7 and NGAL.
Service Facilities in Heritage Tourism: Identification and Planning Based on Space Syntax
Authors: Wang Min , Yang Jianqiang , Hsu Wei-Ling , Zhang Chunmei , Liu Hsin-Lung
Published: December 2021

Improving the development level of tourism service facilities in historic areas of old cities and realizing the sustainable tourism are important strategies for urban historical protection, economic development, and cultural rejuvenation. Districts at different tourism development stages show different characteristics of tourism service facilities. This study collects location-based service data and uses space syntax to identify the correlation between the distribution of tourism service facilities and street networks, which helps decision-makers to optimize the spatial layout of tourism facilities in the planning of historic areas. Taking the southern historic area of Nanjing, China, as an example, this is an area with a rich collection of cultural heritage and many historic districts, and the study reveals that the areas with strongest street agglomeration and best accessibility, as well as the districts with most mature tourism development, are the core of the tourism facilities. The agglomeration of transportation and accommodation facilities should be set at the traffic nodes as much as possible due to the highest correlation with the street network. Instead, the entertainment, catering, and shopping facilities can be set in the nontraffic node areas under the premise of ensuring good traffic accessibility owing to the insignificantly relationship with the street network. The research results can be used as an important reference for urban decision-makers regarding the planning of historic areas.
Olean (1,7-dioxaspiro[5.5]undecane): A Novel Intraspecific Chemical Cue in Coraebus undatus (F.) (Coleoptera: Buprestidae)
Authors: López Sergio , Álvarez-Calero José María , Riba-Flinch Josep Maria , Coca-Abia María Milagro , Torrell Antoni , Quero Carmen
Published: December 2021

The main aim of this work was to identify semiochemicals from the jewel beetle Coraebus undatus (F.) (Coleoptera: Buprestidae) that may aid in the improvement of current monitoring tools. First, HS-SPME collections revealed that individually sampled adults (>7 days old) of both sexes release the spiroacetal 1,7-dioxaspiro[5.5]undecane (olean). Electroantennographic recordings from both sexes exposed to increasing amounts of olean followed a dose-dependent pattern, with females being more responsive than males to the highest amount of the compound (100 µg). In double-choice assays, adults older than seven days were significantly attracted to olean, whereas this attraction was not detected in insects aged less than seven days. Indeed, a repellent effect was observed in young females. Subsequent field trials employing sticky purple prism traps revealed that there were no differences among the number of insects caught in control and olean-baited traps at two different release rates (0.75 and 3.75 mg/day). Interestingly, all the trapped specimens were determined as mated females, regardless of the presence of olean. Overall, these findings provide a basis for unraveling the chemical ecology of the species, although further research is still needed to determine the specific role of this compound within the chemical communication of the species.
Improving the Knowledge on Distribution, Food Preferences and DNA Barcoding of Natura 2000 Protected Species Paracossulus thrips (Lepidoptera, Cossidae) in Romania
Authors: Iacob Geanina , Craioveanu Cristina , Hula Vladimír , Aurelian Virgiliu , Beldean Monica , Sitar Cristian
Published: December 2021

Paracossulus thrips (Lepidoptera, Cossidae) is one of the locally distributed and endangered species. In Europe, it is also one of the few protected moth species, through Annexes II and IV of the Council Directive 92/43/EEC, Annex II of the Bern Convention. To date, little is known about the biology and ecology of this species. Our study was conducted in Transylvania, Romania. Romania hosts some of the strongest populations of the species in the European region. As part of the study, we conducted field observations, vegetation analyses, and genetic analyses. In our paper, we show the habitat types where we encounter P. thrips in Transylvania and confirm Phlomis tuberosa as a host plant. Furthermore, a piece of important information for habitat conservation is given. In this paper, we present the eggs and larvae of P. thrips, the first DNA barcoding sequences, and four new populations of P. thrips in Romania. Our study provides baseline knowledge about the biology and ecology of P. thrips, which is important for conservation and establishing management measures.
Impact of Co-Existing Placental Pathologies in Pregnancies Complicated by Placental Abruption and Acute Neonatal Outcomes
Authors: Mavedatnia Dorsa , Tran Jason , Oltean Irina , Bijelić Vid , Moretti Felipe , Lawrence Sarah , El Demellawy Dina
Published: December 2021

Placental abruption (PA) is a concern for maternal and neonatal morbidity. Adverse neonatal outcomes in the setting of PA include higher risk of prematurity. Placental pathologies include maternal vascular malperfusion (MVM), fetal vascular malperfusion (FVM), acute chorioamnionitis, and villitis of unknown etiology (VUE). We aimed to investigate how placental pathology contributes to acute neonatal outcome in PA. A retrospective cohort study of all placentas with PA were identified. Exposures were MVM, FVM, acute chorioamnionitis and VUE. The primary outcome was NICU admission and the secondary outcomes included adverse base deficit and Apgar scores, need for resuscitation, and small-for-gestational age. A total of 287 placentas were identified. There were 160 (59.9%) of placentas with PA alone vs 107 (40.1%) with PA and additional placental pathologies. Odds of NICU admission were more than two times higher in pregnancies with placental pathologies (OR = 2.37, 95% CI 1.28–4.52). These estimates were in large part mediated by prematurity and birthweight, indirect effect acting through prematurity was OR 1.79 (95% CI 1.12–2.75) and through birthweight OR 2.12 (95% CI 1.40–3.18). Odds of Apgar score ≤ 5 was more than four times higher among pregnancies with placental pathologies (OR = 4.56, 95% CI 1.28–21.26). Coexisting placental pathology may impact Apgar scores in pregnancies complicated by PA. This knowledge could be used by neonatal teams to mobilize resources in anticipation of the need for neonatal resuscitation.
Association between Rheumatoid Arthritis and Meniere’s Disease: A Longitudinal Follow-Up Study Using a National Health Screening Cohort
Authors: Kim So Young , Yoo Dae Myoung , Kim Ji Hee , Kwon Mi Jung , Kim Joo-Hee , Choi Hyo Geun
Published: December 2021

This study aimed to evaluate the impact of pre-existing rheumatoid arthritis (RA) on the occurrence of Meniere’s disease (MD). The 2002–2015 Korean National Health Insurance Service—Health Screening Cohort data were retrospectively analyzed. A total of 3038 participants with RA were matched with 12,152 control participants for demographic factors. The occurrence of MD was evaluated in both the RA and control groups. The hazard ratios (HRs) of RA for participants with MD were calculated using a stratified Cox proportional hazard model. Additionally, subgroup analyses were conducted. The rate of MD was not different between the RA and control groups (1.5% vs. 1.3%, standardized difference = 0.01). The HR was not higher in the RA group than in the MD group (adjusted HR = 1.03, 95% confidence interval = 0.73–1.44, p = 0.885). A higher HR of RA for participants with MD was found in the ≥60-year-old subgroup in the crude model but not in the adjusted model. An association between RA and MD was not found in any of the other subgroups. A previous history of RA was not related to an increased risk of MD.
The Role of Rehabilitative Ultrasound Imaging Technique in the Lumbopelvic Region as a Diagnosis and Treatment Tool in Physiotherapy: Systematic Review, Meta-Analysis and Meta-Regression
Authors: Fernández-Carnero Samuel , Martin-Saborido Carlos , Achalandabaso Ochoa-Ruiz de Mendoza Alexander , Ferragut-Garcias Alejandro , Cuenca-Zaldivar Juan Nicolás , Leal-Quiñones Alejandro , Calvo-Lobo Cesar , Gallego-Izquierdo Tomas
Published: December 2021

Rehabilitative ultrasound imaging (RUSI) technique seems to be a valid and reliable tool for diagnosis and treatment in physiotherapy and has been widely studied in the lumbopelvic region the last three decades. The aims for this utility in clinical settings must be review through a systematic review, meta-analysis and meta-regression. A systematic review was designed following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines with PROSPERO registration and per review in all phases of the process using COVIDENCE, analysis of risk of bias and meta-analysis using REVMAN, and meta-regression calculation using STATA. Database screening provided 6544 references, out of which 321 reported narrative synthesis, and 21 reported quantitative synthesis, while only 7 of them provided comparable data to meta-analyze the variables pain and muscle thickness. In most cases, the forest plots showed considerable I2 heterogeneity indexes for multifidus muscle thickness (I2 = 95%), low back pain (I2 = 92%) and abdominal pain (I2 = 95%), not important for transversus abdominis muscle thickness (I2 = 22%), significant heterogenity (I2 = 69%) depending on the subgroup and not important internal oblique muscle thickness (I2 = 0%) and external oblique muscle thickness (I2 = 0%). Meta-regression did not provide significant data for the correlations between the variables analyzed and the intervention, age, and BMI (Body Mass Index). This review reveals that RUSI could contribute to a high reliability of the measurements in the lumbopelvic region with validity and reliability for the assessments, as well as showing promising results for diagnosis and intervention assessment in physiotherapy compared to the traditional model, allowing for future lines of research in this area.
Measuring Patient Value after Total Shoulder Arthroplasty
Authors: Lädermann Alexandre , Eurin Rodolphe , Alibert Axelle , Bensouda Mehdi , Bothorel Hugo
Published: December 2021

Evaluating the value of health care is of paramount importance to keep improving patients’ quality of life and optimizing associated costs. Our objective was to present a calculation method based on Michael Porter’s formula and standard references to estimate patient value delivered by total shoulder arthroplasty (TSA). We retrospectively reviewed the records of 116 consecutive TSAs performed between June 2015 and June 2019. Patient value was defined as quality of care divided by direct costs of surgery. Quality metrics included intra- and postoperative complications as well as weighted improvements in three different patient-reported outcome measures at a minimum of one-year follow-up. Direct costs of surgery were retrieved from the management accounting analyses. Substantial clinical benefit (SCB) thresholds and the standard reimbursement system were used as references for quality and cost dimensions. A multivariable linear regression was performed to identify factors associated with patient delivered value. Compared to a reference of 1.0, the quality of care delivered to patients was 1.3 ± 0.3 (range, 0.6–2.0) and the associated direct cost was 1.0 ± 0.2 (range, 0.7–1.6). Ninety patients (78%) had a quality of care ≥1.0 and 61 patients (53%) had direct costs related to surgery ≤1.0. The average value delivered to patients was 1.3 ± 0.4 (range, 0.5–2.5) with 91 patients (78%) ≥ 1.0, was higher for non-smokers (beta, 0.12; p = 0.044), anatomic TSA (beta, 0.53; p < 0.001), increased with higher pre-operative pain (beta, 0.08; p < 0.001) and lower pre-operative Constant score (beta, −0.06; p = 0.001). Our results revealed that almost 80% of TSAs provided substantial patient value. Patient pre-operative pain/function, tobacco use, and procedure type are important factors associated with delivered patient value.
Special Issue “Pathogenesis, Epidemiology and Treatment of Atopic Dermatitis and Psoriasis”
Authors: Furue Masutaka
Published: December 2021

Atopic dermatitis and psoriasis are common inflammatory skin diseases that enormously deteriorate the psycho-physical and socio-economic condition of the patients who are afflicted with these conditions [...]
Focus on Key Issues in Immune Thrombotic Thrombocytopenic Purpura: Italian Experience of Six Centers
Authors: Tiscia Giovanni , Sartori Maria Teresa , Giuffrida Gaetano , Ostuni Angelo , Cascavilla Nicola , Nicolosi Daniela , Battista Cosima , Santeramo Teresa Maria , Melillo Lorella , Giordano Giulio , Cappucci Filomena , Fischetti Lucia , Chinni Elena , Tarantini Giuseppe , Cerbo Anna , Bertomoro Antonella , Fabris Fabrizio , Grandone Elvira
Published: December 2021

Immune-mediated thrombotic thrombocytopenic purpura is a rare and challenging hematological disease caused by the antibody anti-ADAMTS13. Though the mortality rate has decreased considerably in recent years, fatalities still remain unacceptable. This study aimed at further adding to the existing knowledge of this medical challenge. We enrolled 89 consecutive patients observed in six Italian centers (from 8 August 2013 to 28 May 2021) with a diagnosis of immune-mediated thrombotic thrombocytopenic purpura. Clinical information and blood parameters were collected for all patients. We describe clinical manifestations and laboratory data, possible risk factors and the therapeutic management of first episodes or relapses. A total of 74 first episodes and 19 relapses (median 3 years (interquartile range (IQR): 2–7)) were recorded. Seventy percent of patients enrolled at the first episode showed neurological signs and/or symptoms. All the patients enrolled at the first episode were treated with plasma exchange (median = 12; IQR: 8–19.5) and methylprednisolone (1 mg/kg/day). Rituximab (375 mg/m2 weekly for four weeks) and caplacizumab were given to 15 (20.2%) and 2 patients (2.6%), respectively. We observed an overall mortality of 5.4% in the follow-up (median 60 months; IQR: 36.0–103.5). All fatalities occurred after a diagnostic delay. Present data point to the importance of the early detection of factors mostly associated with poor outcomes. It is likely that use of caplacizumab could improve the prognosis in those patients.
Radiological Cardiothoracic Ratio as a Potential Predictor of Right Ventricular Enlargement in Patients with Suspected Pulmonary Embolism Due to COVID-19
Authors: Truszkiewicz Krystian , Poręba Małgorzata , Poręba Rafał , Gać Paweł
Published: December 2021

The aim of the study was to determine the usefulness of the radiological cardiothoracic ratio (CTR) as a predictor of right ventricular enlargement in patients with suspected pulmonary embolism during COVID-19. The study group consisted of 61 patients with confirmed COVID-19, suspected of pulmonary embolism based on physical examination and laboratory tests (age: 67.18 ± 12.47 years). Computed tomography angiography (CTA) of pulmonary arteries and chest radiograph in AP projection with cardiothoracic ratio assessment were performed in all patients. Right ventricular enlargement was diagnosed by the ratio of right ventricular to left ventricular (RV/LV) dimensions in pulmonary CTA with two cut-off points: ≥0.9 and ≥1.0. Heart silhouette enlargement was found when CTR on the chest radiograph in the projection AP > 0.55. The mean values of RV/LV and CTR in the studied group were 0.96 ± 0.23 and 0.57 ± 0.05, respectively. Pulmonary embolism was diagnosed in 45.9%. Right ventricular enlargement was documented in 44.3% or 29.5% depending on the adopted criterion RV/LV ≥ 0.9 or RV/LV ≥ 1.0. Heart silhouette enlargement was found in 60.6%. Patients with confirmed pulmonary embolism (PE+) had a significantly higher RV/LV ratio and CTR than patients with excluded pulmonary embolism (PE−) (RV/LV: PE+ 1.08 ± 0.24, PE− 0.82 ± 0.12; CTR: PE+ 0.60 ± 0.05, PE− 0.54 ± 0.04; p < 0.05). The correlation analysis showed a statistically significant positive correlation between the RV/LV ratio and CTR (r = 0.59, p < 0.05). Based on the ROC curves, CTR values were determined as the optimal cut-off points for the prediction of right ventricular enlargement (RV/LV ≥ 0.9 or RV/LV ≥ 1.0), being 0.54 and 0.55, respectively. The sensitivity, specificity, and accuracy of the CTR criterion >0.54 as a predictor of RV/LV ratio ≥0.9 were 0.412, 0.963, and 0.656, respectively, while those of the CTR criterion >0.55 as a predictor of RV/LV ratio ≥1.0 were 0.488, 0.833, and 0.590, respectively. In summary, in patients with suspected pulmonary embolism during COVID-19, the radiographic cardiothoracic ratio can be considered as a prognostic factor for right ventricular enlargement, especially as a negative predictor of right ventricular enlargement in the case of lower CTR values.
Gastric Xanthoma Is Related to the Rapid Growth of Gastric Cancer
Authors: Miura Ko , Oshima Tadayuki , Tamura Akio , Hara Ken , Okugawa Takuya , Fukushima Masashi , Tomita Toshihiko , Fukui Hirokazu , Miwa Hiroto
Published: December 2021

Early detection of gastric cancer is important. However, rapid growth of gastric cancers that cannot be resected endoscopically occurs even with periodic check-ups. Accordingly, we assessed factors associated with the speed of gastric cancer growth by examining historical endoscopic images. A total of 1996 gastric cancer cases were screened, and characteristics of lesions with slow and rapid growth were assessed. A total of 114 lesions from 114 patients were included in the assessment. Sixty slow-growing and fifty-four rapidly growing gastric cancers were compared. Female sex and incidence of lesions in the lower part of the stomach were significantly less frequent in the rapid-growth group than in the slow-growth group. History of endoscopic treatment tended to be more frequent in the rapid-growth group. Age, body mass index, histology, Helicobacter pylori status, and medications did not differ significantly between groups. Xanthoma was significantly related to rapid growth of gastric cancer, and map-like redness tended to be more frequent in the rapid-growth group in univariate analysis. Xanthoma was significantly related to rapid growth of gastric cancer on multivariate analysis. Further studies are warranted to clarify the pathophysiological mechanisms involved in the speed of gastric cancer growth.
Retinal Hemorrhage after SARS-CoV-2 Vaccination
Authors: Park Hyo Song , Byun Yeojue , Byeon Suk Ho , Kim Sung Soo , Kim Yong Joon , Lee Christopher Seungkyu
Published: December 2021

To report retinal vein occlusion (RVO) and age-related macular degeneration (AMD)-related submacular hemorrhage developing after administration of SARS-CoV-2 vaccines, a single-center, retrospective observational case series was conducted. Clinical data including fundus photographs and optical coherence tomography (OCT) scans were reviewed. Twenty-three eyes of 21 patients were included with the median age at symptom presentation being 77 years (range: 51–85 years). Twelve eyes (52.2%) had submacular hemorrhage and 11 (47.8%) had RVO. Twelve patients (60.9%) had been vaccinated with the Pfizer vaccine (BNT162b2) and 8 with the AstraZeneca (ChAdOx1) vaccine. Sixteen patients (76.2%) experienced ocular disease exacerbation after the first vaccination and 4 (19.0%) after the second vaccination. The median visual acuity (logarithm of the minimal angle of resolution; logMAR) before symptom development was 0.76 (interquartile range: 0.27–1.23); the median logMAR at symptom presentation was 1.40 (interquartile range 0.52–1.70). The median time between vaccination and symptom exacerbation was 2.0 days (interquartile range: 1.0–3.0 days). Five patients (23.8%) underwent tests for hematological abnormalities, including the presence of anti-PF4 antibodies; all were negative. Further studies with larger patient group for evaluation of effect of SARS-CoV-2 vaccination on retinal hemorrhage are necessary.
Multi-Objective Optimisation of the Benchmark Wind Farm Layout Problem
Authors: Manikowski Pawel L. , Walker David J. , Craven Matthew J.
Published: December 2021

Wind farm layout optimisation has become a very challenging and widespread problem in recent years. In many publications, the main goal is to achieve the maximum power output and minimum wind farm cost. This may be accomplished by applying single or multi-objective optimisation techniques. In this paper, we apply a single objective hill-climbing algorithm (HCA) and three multi-objective evolutionary algorithms (NSGA-II, SPEA2 and PESA-II) to a well-known benchmark optimisation problem proposed by Mosetti et al., which includes three different wind scenarios. We achieved better results by applying single- and multi-objective algorithms. Furthermore, we showed that the best performing multi-objective algorithm was NSGA-II. Finally, an extensive comparison of the results of past publications is made.