bluuebunny/crossref_metadata_2025 huggingface dataset:
How Do EMI Lecturers’ Translanguaging Perceptions Translate into Their Practice? A Multi-Case Study of Three Chinese Tertiary EMI Classes
Authors: Jia Wenyun , Fu Xuehua , Pun Jack
Published: March 2023

Translanguaging is discussed widely in the literature as a new pedagogical tool for English-medium instruction (EMI) that can challenge monolingual assumptions, mitigate English language barriers, and advocate for the full use of linguistic resources in meaning making. Different EMI lecturers have been found to hold diverse translanguaging perceptions and have adopted a variety of its functions. However, more needs to be known to close the knowledge gap between how each lecturer envisions and practices their translanguaging pedagogies, if any. Employing a qualitative method using data from semi-structured interviews and classroom observation, this study investigated three Chinese tertiary EMI engineering lecturers’ translanguaging perceptions and practices. Taken together, these three cases illustrate the complex relationship between translanguaging perceptions and practices according to their interactional (e.g., students’ difficulties in understanding full EMI), socio-cultural (e.g., the socially constructed value of full EMI), and personal factors (e.g., EMI teaching experiences). We propose that the negotiation among these three domains can promote or withhold the transfer of translanguaging perceptions into practice. We use our findings to call for EMI training programs to encourage lecturers’ reflections regarding what languages are or can be used in EMI classrooms and how and why from socio-cultural, personal, and interactional perspectives.
How to Measure the Impact of Walking Accessibility of Suburban Rail Station Catchment Areas on the Commercial Premium Benefits of Joint Development
Authors: Qin Yuchen , Zhang Yikang , Yao Minfeng , Chen Qiwei
Published: March 2023

As the primary solution to the issue of high passenger traffic in urban areas, rail transit has a significant impact on the structural form of cities and regional development. Additionally, it has varying degrees of a premium effect on land value around stations. Current research on the factors influencing the premium effect of rail transit station areas mainly focuses on the macro level of the station area circle, with more attention given to the premium caused by distance and functional differences. Most research objects are typically urban center lines or stations. However, this study focuses on the core area of the station and concentrates on the impact of the construction of integrated station–city facilities on the choice of pedestrian routes and the enhancement of pedestrian accessibility. It also explores whether this enhancement is associated with the premium benefits of ancillary commercial development. To achieve this goal, this paper integrates models from several related studies to conduct a comprehensive assessment. Firstly, it uses a spatial panel econometric model to improve the classical characteristic price method model. It then combines the ideas and models of the cost–benefit analysis method, taking the Odakyu Odawara Line of the Japanese suburban railroad as an example. This analysis explores the mechanism and factors influencing the rent premium of commercial facilities in the suburban rail station area and systematically assesses the combined station–city facilities. The study evaluates the social benefits (enhanced walkability) and economic value (premium value added from commercial facilities) of the combined station–city facilities systematically. The results of the study show that (1) the premium benefits of suburban railroad station area commercial facilities are significantly related to the type of station–city combination facilities, combination mode, and walking time and weakly related to the location factor. Additionally, (2) the results of the cost–benefit valuation analysis based on the Ebina Station verify that a reasonable design of station–city combination facilities can effectively enhance the proximity of commercial facilities to the station and improve the walking accessibility, thus promoting the premium benefits. The study demonstrates that a reasonable design of the combined station and city facilities can effectively enhance the proximity of commercial facilities to the station and improve pedestrian accessibility, promoting premium benefits, which can quickly feed the construction cost of the station and achieve positive revenue in the short term. Therefore, the results of the study provide a quantitative reference for the planning and design of suburban stations.
Mapping Connections between Neighborhoods in Response to Community-Based Social Needs
Authors: Bevilacqua Carmelina , Sohrabi Poya , Hamdy Nourhan , Mangiulli Federica
Published: March 2023

Geographic proximity might not be the only factor influencing the connections between neighborhoods within the same city. Most likely, the community’s needs and behaviors play a role in facilitating or hindering any connections between these urban areas. Accordingly, relationships between communities may differ or be similar based on their respective characteristics. This paper aims to demonstrate that communities are close based on the needs they share, regardless of their ethnicity or geographic location. In this study, a time series analysis of neighborhoods’ needs is explored to gain a deeper understanding of the communities’ network. The study takes into account the co-occurrence of complaints/reports from residents regarding the same issue. The dataset was retrieved from the Boston Area Research Initiative (BARI) and the 311 system that describe the features of neighborhoods regarding non-emergency issues. Subsequently, the connection between neighborhoods in the City of Boston was analyzed using a mixture of PCA, K-means, association rule mining, and a network creation tool. Moreover, clustering coefficients and degrees of centrality were used as significant factors in identifying the members of groups and marking crucial nodes in the network. A series of graphs were generated to show how the neighborhoods are linked based on their socioeconomic concerns. The results prove that even geographically disconnected neighborhoods within Boston have similar social needs, despite their distance from one another. Furthermore, it revealed that some neighborhoods can act as linking bridges for other neighborhoods, while others may be isolated within the network graph. This study has increased awareness of urban aspects. The authorities may consider other dimensions than the traditional ones regarding neighborhood development and addressing problems. Finally, it helps to identify common characteristics between neighborhoods, which facilitates the policy making process.
Property Improvement of Recycled Coarse Aggregate by Accelerated Carbonation Treatment under Different Curing Conditions
Authors: Yang Shiqing , Gu Mingjie , Lin Hongyi , Gong Yue
Published: March 2023

Recycled aggregate (RA) made from waste concrete has inferior fundamental properties, i.e., apparent density, water absorption, mass variation, carbonation ratio, etc., compared to those of natural aggregate (NA), severely restricting its application in practical projects. However, using CO2 to accelerate RA carbonation can effectively improve these properties, and this treatment approach can promote energy savings and sustainable development. The accelerated carbonation curing conditions for RA can significantly impact the modification effect of RA. For this purpose, this paper used recycled coarse aggregate (RCA) as a case study. An accelerated carbonation modification treatment experiment for RCA under different accelerated carbonation curing conditions was carried out, and the effects of relative humidity and CO2 concentration on the apparent density, water absorption, moisture content, mass variation and carbonation ratio of RCA under a constant ambient temperature were explored and quantified. The results indicated that the best-accelerated carbonation curing conditions applicable to this paper’s RCA were confirmed as being an environmental temperature of 20 °C and a relative humidity of 70%, as well as a CO2 concentration of 20%. Under these conditions, the apparent density and water absorption of CRCA are approximately 1.04 times and 75.30% higher than those of RCA, and, in addition, the carbonation ratio for RCA under the optimal accelerated carbonation curing conditions is all higher than others, thus improving the properties of RCA to a certain degree. Finally, in this paper, the variation trends of the RCA property indexes in terms of carbonation time treated by the best accelerated carbonation curing conditions are examined, and the time-varying models for the RCA property indexes during the accelerated carbonation are established.
Interactions between Farmers’ Adaptation Strategies to Climate Change and Sustainable Development Goals in Tanzania, East Africa
Authors: Magesa Bahati Ally , Mohan Geetha , Melts Indrek , Matsuda Hirotaka , Pu Jian , Fukushi Kensuke
Published: March 2023

This study investigated the potential adaptation measures for farmers in the Mwanga and Same Districts of Tanzania and then assessed their positive and negative interactions towards potential contributions to the selected sustainable development goal (SDG) indicators of no poverty and zero hunger. A total of 200 household surveys were conducted, and 36 participants were interviewed as key informants. Moreover, four focus group discussions were conducted to identify potential adaptation strategies in the studied areas. The literature and expert judgement approaches were used to understand and assess the positive and negative interactions between adaptation strategies and the selected indicators of SDGs. A seven-point scale of SDG interactions was used to determine the interactions between identified adaptation strategies and selected SDG indicators. Qualitative data were subjected to content analysis, whereas quantitative data were analyzed through descriptive statistics. The finding revealed that some of the adaptation strategies (i.e., stream bank crop cultivation, valley bottom crop cultivation, and cultivating crops near water sources) considered potential at the household levels and had strong negative interactions on achieving SDG 2.4.1. Further, most farmers hardly employed strategies (i.e., use of extension officers, concrete irrigation channels, crop insurance schemes, and credit schemes) that had strong positive interactions on the selected SDG indicators. Moreover, most of the identified strategies (i.e., early maturity crops, planting drought-resistant crops, use of improved varieties, mixing improved and local varieties, mixing short and long duration varieties, and crop diversification) are enabling strategies (+1), which, despite their importance, may constrain (−1) the income of small-scale farmers, food security, and poverty reduction. The importance of other strategies (i.e., irrigation infrastructures) must be addressed for better yields and positive impacts. Hence, achieving SDGs 1 and 2 in the studied areas will require the integration of different adaptation strategies that complement each other, and not by promoting only some strategies as used or suggested before. For example, the emphasis on using improved varieties and crop diversification should be complemented by access to credit schemes, irrigation infrastructures, crop insurance, and extension services at the village level.
The Impact of Economic Policy Uncertainty on Enterprise Green Innovation: A Study on the Moderating Effect of Carbon Information Disclosure
Authors: Luo Xu , Yu Mengke , Jin Yongsheng
Published: March 2023

In the context of achieving carbon peaking and carbon neutrality, China has introduced a series of policies to encourage enterprises to adopt green innovation behavior. However, enterprises are faced with an uncertain policy environment surrounding green innovation decision-making; the mechanisms that influence these decisions are poorly understood; and the regulatory role of carbon information disclosure quality against the background of the dual carbon goals is unclear. We found that the increase in economic policy uncertainty is not conducive to enterprises’ decision-making on green innovation. However, an increase in the quality of carbon information disclosure can promote green innovation in enterprises. Additionally, the quality of carbon information disclosure plays a moderating role in economic policy uncertainty and corporate green innovation. Enterprises can mitigate the negative impact of economic policy uncertainty on corporate green innovation by ensuring high-quality carbon information disclosure to adapt to national policies and improve the level of innovation.
Do We Consider Sustainability When We Measure Small and Medium Enterprises’ (SMEs’) Performance Passing through Digital Transformation?
Authors: Costa Melo Isotilia , Alves Junior Paulo Nocera , Queiroz Geandra Alves , Yushimito Wilfredo , Pereira Jordi
Published: March 2023

Small-medium enterprises (SMEs) represent 90% of business globally. Digital Transformation (DT) affects SMEs differently from larger companies because although SMEs have more flexibility and agility for adapting to new circumstances, they also have more limited resources and specialization capabilities. Thus, it is fundamental to measure SMEs’ performance considering different perspectives. Here, we describe and analyze the state-of-the-art of DT in SMEs, focusing on performance measurement. We center on whether the tools used by SMEs encompass the triple bottom line of sustainability (i.e., environmental, social, and economic aspects). To do so, in December 2021, we performed a comprehensive systematic literature review (SLR) on the Web of Science and Scopus. In addition, we also explored a novel approach for SLR: topic modeling with a machine learning technique (Latent Dirichlet Allocation). The differences and interchangeability of both methods are discussed. The findings show that sustainability is treated as a separate topic in the literature. The social and environmental aspects are the most neglected. This paper contributes to sustainable development goals (SDGs) 1, 5, 8, 9, 10, and 12. A conceptual framework and future research directions are proposed. Thus, this paper is also valuable for policymakers and SMEs switching their production paradigm toward sustainability and DT.
Responsible Leadership and Innovation during COVID-19: Evidence from the Australian Tourism and Hospitality Sector
Authors: Yildiz Mehmet , Pless Nicola , Ceyhan Semih , Hallak Rob
Published: March 2023

The COVID-19 crisis provides an opportunity for sustainable renewal and requires responsible leaders who are responsive to stakeholder needs and able to innovate in light of new challenges. This study draws on stakeholder theory and responsible leadership theory to examine (a) the challenges industry leaders face as a result of COVID-19 and (b) their innovative responses in light of their responsibilities to stakeholders and society. We conducted a corpus linguistics study based on high-volume media websites reporting tourism and hospitality news on leadership and innovation. We applied a stakeholder and social responsibility lens to the data analysis. We discovered that, despite the challenges that leaders faced, some transcended self-interest or integrated self-interest with consideration for the interest of others and formed partnerships with other stakeholders resulting in win-win solutions. In particular, we found evidence of leaders who (1) responded to the needs of owners, employees, customers, and community stakeholders and (2) developed not only incremental innovations but substantial ones benefitting stakeholders in business and society. We discuss responsible leadership as a pathway for transforming the tourism and hospitality industry towards a more sustainable and community-centred ‘new normal’. Based on our findings, we present recommendations for future research and policymakers.
Impact of Agricultural Land Use Types on Soil Moisture Retention of Loamy Soils
Authors: Czigány Szabolcs , Sarkadi Noémi , Lóczy Dénes , Cséplő Anikó , Balogh Richárd , Fábián Szabolcs Ákos , Ciglič Rok , Ferk Mateja , Pirisi Gábor , Imre Marcell , Nagy Gábor , Pirkhoffer Ervin
Published: March 2023

Increasingly severe hydrological extremes are predicted for the Pannonian Basin as one of the consequences of climate change. The challenges of extreme droughts require the adaptation of agriculture especially during the intense growth phase of crops. For dryland farming, the selections of the optimal land use type and sustainable agricultural land management are potential adaptation tools for facing the challenges posed by increased aridity. To this end, it is indispensable to understand soil moisture (SM) dynamics under different land use types over drought-affected periods. Within the framework of a Slovenian–Hungarian project, soil moisture, matric potential and rainfall time series have been collected at three pilot sites of different land use types (pasture, orchards and a ploughland) in SW Hungary since September 2018. Experiments were carried out in soils of silt, silt loam and clay loam texture. In the summers (June 1 to August 31) of 2019 and 2022, we identified normal and dry conditions, respectively, with regard to differences in water balance. Our results demonstrated that soil moisture is closely controlled by land use. Marked differences of the moisture regime were revealed among the three land use types based on statistical analyses. Soils under pasture had the most balanced regime, whereas ploughland soils indicated the highest amplitude of moisture dynamics. The orchard, however, showed responses to weather conditions in sharp contrast with the other two sites. Our results are applicable for loamy soils under humid and subhumid temperate climates and for periods of extreme droughts, a condition which is expected to be the norm for the future.
Alkali–Silica Reactivity Potential of Reactive and Non-Reactive Aggregates under Various Exposure Conditions for Sustainable Construction
Authors: Abbas Safeer , Jabeen Farwa , Faisal Adeel , Nehdi Moncef L. , Kazmi Syed Minhaj Saleem , Mubin Sajjad , Shaukat Sbahat , Munir Muhammad Junaid
Published: March 2023

The alkali–silica reaction (ASR) is a primary cause for premature concrete degradation. An accelerated mortar bar test is often used to access the detrimental phenomena in concrete caused by the ASR of aggregates. However, this test requires a certain environmental conditioning as per ASTM C1260. The objective of this study is to explore the effects of the cement alkali content, exposure solution concentration, temperature, and test duration on mortar bar expansion. Factorial experimental design and analysis was conducted to delineate the effects of the individual factors as well as their interaction. Five different aggregates with various mineralogical properties were used, representing reactive and non-reactive aggregates. Various dosages of cement alkalis (0.40, 0.80, and 1.20 Na2Oe), sodium hydroxide (NaOH) solution concentrations (0.5, 1.0, and 1.5 N), and temperature (40 °C, 80 °C, and 100 °C) were the studied variables. Mortar bar expansion was measured at 3, 7, 14, 21, 28, 56, and 90 days. Mortar bars incorporating Jhelum aggregates incurred expansion of 0.32% at 28 days, proving to be reactive aggregates as per ASTM C1260. Similarly, specimens incorporating Taxila aggregates showed expansion of 0.10% at 28 days, indicating non-reactive nature. It was observed that specimens with Sargodha aggregates showed expansion of 0.27% at 28 days for 0.50 N NaOH solution concentration compared to 0.31% expansion for identical specimens exposed to 1.5 N solution. Moreover, expansion increased with exposure duration for all the tested specimens. Experimental results showed that the cement alkali contents had relatively lesser effect on expansion for 1.0 N NaOH; while, in the case of 0.5 N and 1.5 N NaOH, the cement alkali had a significant effect. It was noted that expansion increased with an increase in the temperature. Jhelum aggregates showed 28-day expansion of 0.290% when exposed to 40 °C, but at a temperature of 100 °C, expansion increased to 0.339%. Factorial analysis revealed that the exposure solution had a major contribution towards the expansion of mortar bar specimens. This study highlights the contribution of various exposure conditions on the ASR expansion, which leads to a decisive role in selecting the aggregate sources for various applications and exposure conditions leading to sustainable construction.
Borich’s Needs Model Analysis of Smallholder Farmers’ Competence in Irrigation Water Management: Case Study of Nkomazi Local Municipality, Mpumalanga Province in South Africa
Authors: Mabuza Mfanufikile , Ndoro Jorine T.
Published: March 2023

Irrigated agriculture enables production intensification and crop diversification to improve food security. However, increasing irrigation water stress and inadequate competence of smallholder farmers in irrigation water management have the potential to exacerbate food insecurity. Therefore, this study seeks to determine smallholder farmers’ competency needs in irrigation water management practices (IWMP). A convenience sampling method was employed to obtain a sample population of n= 250. Descriptive statistics were employed to describe smallholder farmers’ demographic characteristics. Borich’s Needs Assessment Model (BNAM) was utilised to analyse smallholder farmers’ competency needs. Results revealed that smallholder farmers perceived weed control (M = 4.90) and understanding the consequences of over- and under-irrigation (M = 4.48) as highly important practices. Results also revealed that smallholder farmers were only highly competent in weed control (M = 4.59). Moreover, results revealed that the top two most important competency needs for smallholder farmers are knowledge of drought-tolerant cultivars (MWDS = 6.83) and irrigation scheduling (MWDS = 5.05). From the survey findings, smallholder farmers’ competency in IWMP is insufficient. It is recommended that the government, policymakers, and agricultural support services embark on sustainable agricultural development planning issues and develop a relevant training programme that is informed by smallholder farmers’ competency needs.
Vulnerability to Poverty in Chinese Households with Elderly Members: 2013–2018
Authors: Ding Shuo
Published: March 2023

In Chinese households, there is a significant shift away from basing poverty alleviation on the relative poverty line as opposed to the absolute poverty line. Based on VER and the concept of poverty capacities, this study evaluated the impact of development capacities and indicators on the vulnerability to poverty of elderly households. The three most important findings are as follows: (1) This study employed the multidimensional vulnerability methodology to evaluate the effects of potential factors on the Chinese elderly household’s vulnerability. Rather than analysing current poverty, this study focused on the estimation of future poverty, which is prospective. (2) Contrary to the expectations of empirical studies, the application of difference-in-difference and propensity score matching in this study revealed that the government’s institutional pension reform decreases the vulnerability rate further. (3) Both development capacities and indicators are critical determinants of further poverty, and in order to effectively alleviate poverty, policymakers should strengthen capacities and grant rights to vulnerable households.
Purchase Behaviour of Green Footwear in Saudi Arabia Using Theory of Planned Behaviour
Authors: Aseri Mosa , Ansari Zaid Ahmad
Published: March 2023

The study aimed to discover the factors influencing the purchase intention and purchase behaviour of customers for green footwear in the context of Saudi Arabia. The study used the theory of planned behaviour constructs, which was extended with two more variables, environmental consciousness, and health consciousness. It investigated the influence of health consciousness on green purchase intention and attitudes, as well as the influence of environmental consciousness on attitude, subjective norms, perceived behavioural control, and green purchase intention and behaviour of the customers. The results were drawn from empirical data collected from 419 respondents in Saudi Arabia by administering a structured questionnaire. The research model investigated the relationships among constructs by using a structural equation modelling approach. The results show that environmental consciousness influenced attitude, subjective norms, perceived behavioural control, and green purchase intention and behaviour. On the other hand, health consciousness influenced attitudes but showed no significant relationship with the green purchase intention of the customers. Furthermore, environmental consciousness, perceived behavioural control, attitude, and subjective norms showed a statistically significant relationship with green purchase intention for green footwear; however, perceived behavioural control failed to influence green purchase behaviour. The current study is the first of its kind on green footwear using the theory of planned behaviour. Additionally, this is the first study to be conducted in the context of Saudi Arabia. The originality of the study is reflected in the extension of the theory of planned behaviour model with the two constructs of environmental consciousness and health consciousness.
Electrokinetic Remediation of Zn-Polluted Soft Clay Using a Novel Electrolyte Chamber Configuration
Authors: Sun Zhaohua , Tan Wanxia , Gong Jian , Wei Guowei
Published: March 2023

This study investigated a novel electrolyte chamber configuration for heavy-metal-contaminated fine-grained soil to reduce the leakage of electrolyte solution and alleviate secondary pollution, finally promoting the electrokinetic remediation (EKR) potential to be scaled up for application. Experiments were conducted on clay spiked with Zn to investigate the feasibility of the novel EKR configuration and the effect of different electrolyte compositions on the electrokinetic remedial efficiency. The results show that the electrolyte chamber situated above the soil surface is promising for the remediation of Zn-contaminated soft clay. Using 0.2 M citric acid as the anolytes and catholytes was an excellent choice for pH control in the soil and the electrolytes. Through this, the removal efficiency in different soil sections was relatively uniform and more than 90% of the initial Zn was removed. The supplementing of electrolytes resulted in the water content in the soil being distributed evenly and finally sustained at approximately 43%. Consequently, this study proved that the novel EKR configuration is suitable for fine-grained soil contaminated with Zn.
Contamination and Health Risk Assessment of Multiple Mycotoxins in Edible and Medicinal Plants
Authors: Zhang Yingyue , Kuang Fengyan , Liu Chunyao , Ma Kai , Liu Tianyu , Zhao Meijuan , Lv Guangping , Huang He
Published: March 2023

Edible and medicinal plants (EMPs) are widely used but are easily infected by harmful fungi which produce mycotoxins. Herein, 127 samples from 11 provinces were collected to investigate 15 mycotoxins based on geographic, demographic, processing, and risk characteristics. A total of 13 mycotoxins were detected, and aflatoxin B1 (0.56~97.00 μg/kg), deoxynivalenol (9.41~1570.35 μg/kg), fumonisin B1 (8.25~1875.77 μg/kg), fumonisin B2 (2.74~543.01 μg/kg), ochratoxin A (0.62~19.30 μg/kg), and zearalenone (1.64~2376.58 μg/kg) occurred more frequently. Mycotoxin levels and species were significantly different by region, types of EMPs, and method of processing. The margin of exposure (MOE) values was well below the safe MOE (10,000). AFB1 exposure from Coix seed and malt consumption in China was of high health concern. The hazard Index (HI) method showed the range of 113.15~130.73% for malt, indicating a public health concern. In conclusion, EMPs should be concerned because of the cumulative effects of co-occurred mycotoxins, and safety management strategies should be developed in follow-up studies.
Toxinology and Pharmacology of Snake Venoms
Authors: Križaj Igor
Published: March 2023

Evolution endowed snakes with the ultimate weapon: venom [...]
Gene Therapy for β-Hemoglobinopathies: From Discovery to Clinical Trials
Authors: Segura Eva Eugenie Rose , Ayoub Paul George , Hart Kevyn Lopez , Kohn Donald Barry
Published: March 2023

Investigations to understand the function and control of the globin genes have led to some of the most exciting molecular discoveries and biomedical breakthroughs of the 20th and 21st centuries. Extensive characterization of the globin gene locus, accompanied by pioneering work on the utilization of viruses as human gene delivery tools in human hematopoietic stem and progenitor cells (HPSCs), has led to transformative and successful therapies via autologous hematopoietic stem-cell transplant with gene therapy (HSCT-GT). Due to the advanced understanding of the β-globin gene cluster, the first diseases considered for autologous HSCT-GT were two prevalent β-hemoglobinopathies: sickle cell disease and β-thalassemia, both affecting functional β-globin chains and leading to substantial morbidity. Both conditions are suitable for allogeneic HSCT; however, this therapy comes with serious risks and is most effective using an HLA-matched family donor (which is not available for most patients) to obtain optimal therapeutic and safe benefits. Transplants from unrelated or haplo-identical donors carry higher risks, although they are progressively improving. Conversely, HSCT-GT utilizes the patient’s own HSPCs, broadening access to more patients. Several gene therapy clinical trials have been reported to have achieved significant disease improvement, and more are underway. Based on the safety and the therapeutic success of autologous HSCT-GT, the U.S. Food and Drug Administration (FDA) in 2022 approved an HSCT-GT for β-thalassemia (Zynteglo™). This review illuminates the β-globin gene research journey, adversities faced, and achievements reached; it highlights important molecular and genetic findings of the β-globin locus, describes the predominant globin vectors, and concludes by describing promising results from clinical trials for both sickle cell disease and β-thalassemia.
How SARS-CoV-2 Big Data Are Challenging Viral Taxonomy Rules
Authors: Focosi Daniele , Maggi Fabrizio
Published: March 2023

SARS-CoV-2 genomic sequencing has peaked to unprecedented compared to other viruses [...]
Identifying Putative Resistance Genes for Barley Yellow Dwarf Virus-PAV in Wheat and Barley
Authors: Alquicer Glenda , Ibrahim Emad , Maruthi Midatharahally N. , Kundu Jiban Kumar
Published: March 2023

Barley yellow dwarf viruses (BYDVs) are one of the most widespread and economically important plant viruses affecting many cereal crops. Growing resistant varieties remains the most promising approach to reduce the impact of BYDVs. A Recent RNA sequencing analysis has revealed potential genes that respond to BYDV infection in resistant barley genotypes. Together with a comprehensive review of the current knowledge on disease resistance in plants, we selected nine putative barley and wheat genes to investigate their involvement in resistance to BYDV-PAV infection. The target classes of genes were (i) nucleotide binding site (NBS) leucine-rich repeat (LRR), (ii) coiled-coil nucleotide-binding leucine-rich repeat (CC-NB-LRR), (iii) LRR receptor-like kinase (RLK), (iv) casein kinase, (v) protein kinase, (vi) protein phosphatase subunits and the transcription factors (TF) (vii) MYB TF, (viii) GRAS (gibberellic acid-insensitive (GAI), repressor of GAI (RGA) and scarecrow (SCR)), and (ix) the MADS-box TF family. Expression of genes was analysed for six genotypes with different levels of resistance. As in previous reports, the highest BYDV-PAV titre was found in the susceptible genotypes Graciosa in barley and Semper and SGS 27-02 in wheat, which contrast with the resistant genotypes PRS-3628 and Wysor of wheat and barley, respectively. Statistically significant changes in wheat show up-regulation of NBS-LRR, CC-NBS-LRR and RLK in the susceptible genotypes and down-regulation in the resistant genotypes in response to BYDV-PAV. Similar up-regulation of NBS-LRR, CC-NBS-LRR, RLK and MYB TF in response to BYDV-PAV was also observed in the susceptible barley genotypes. However, no significant changes in the expression of these genes were generally observed in the resistant barley genotypes, except for the down-regulation of RLK. Casein kinase and Protein phosphatase were up-regulated early, 10 days after inoculation (dai) in the susceptible wheat genotypes, while the latter was down-regulated at 30 dai in resistant genotypes. Protein kinase was down-regulated both earlier (10 dai) and later (30 dai) in the susceptible wheat genotypes, but only in the later dai in the resistant genotypes. In contrast, GRAS TF and MYB TF were up-regulated in the susceptible wheat genotypes while no significant differences in MADS TF expression was observed. Protein kinase, Casein kinase (30 dai), MYB TF and GRAS TF (10 dai) were all up-regulated in the susceptible barley genotypes. However, no significant differences were found between the resistant and susceptible barley genotypes for the Protein phosphatase and MADS FT genes. Overall, our results showed a clear differentiation of gene expression patterns in both resistant and susceptible genotypes of wheat and barley. Therefore, further research on RLK, NBS-LRR, CC-NBS-LRR, GRAS TF and MYB TF can lead to BYDV-PAV resistance in cereals.
Clinical Presentation and Severity of SARS-CoV-2 Infection Compared to Respiratory Syncytial Virus and Other Viral Respiratory Infections in Children Less than Two Years of Age
Authors: Nunziata Francesco , Salomone Simona , Catzola Andrea , Poeta Marco , Pagano Federica , Punzi Liana , Lo Vecchio Andrea , Guarino Alfredo , Bruzzese Eugenia
Published: March 2023

The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the implementation of restrictive measures led to a dramatic reduction in respiratory syncytial virus (RSV) occurrence together with rare and mild bronchiolitis induced by SARS-CoV-2. We described the respiratory picture of SARS-CoV-2 infection and evaluated the frequency and the severity of SARS-CoV-2 bronchiolitis comparing it with other respiratory viral infections in children less than two years of age. The severity of respiratory involvement was evaluated based on the need for oxygen therapy, intravenous hydration, and the length of hospital stay. A total of 138 children hospitalized for respiratory symptoms were enrolled: 60 with SARS-CoV-2 and 78 with RSV. In the group of SARS-CoV-2-infected children, 13/60 (21%) received a diagnosis of co-infection. Among the enrolled children, 87/138 (63%) received a diagnosis of bronchiolitis. The comparative evaluation showed a higher risk of the need for oxygen therapy and intravenous hydration in children with RSV infection and co-infection compared to children with SARS-CoV-2 infection. In the children with a diagnosis of bronchiolitis, no differences in the main outcomes among the groups were observed. Although children with SARS-CoV-2 infection have less severe respiratory effects than adults, the pediatrician should pay attention to bronchiolitis due to SARS-CoV-2, which could have a severe clinical course in younger children.
Impact Assessment of vB_KpnP_K1-ULIP33 Bacteriophage on the Human Gut Microbiota Using a Dynamic In Vitro Model
Authors: Laforêt Fanny , Antoine Céline , Lebrun Sarah , Gonza Irma , Goya-Jorge Elizabeth , Douny Caroline , Duprez Jean-Noël , Scippo Marie-Louise , Taminiau Bernard , Daube Georges , Fall Abdoulaye , Thiry Damien , Delcenserie Véronique
Published: March 2023

New control methods are needed to counter antimicrobial resistances and the use of bacteriophages as an alternative treatment seems promising. To that end, the effect of the phage vB_KpnP_K1-ULIP33, whose host is the hypervirulent Klebsiella pneumoniae SA12 (ST23 and capsular type K1), was assessed on intestinal microbiota, using an in vitro model: the SHIME® system (Simulator of the Human Intestinal Microbial Ecosystem). After stabilization of the system, the phage was inoculated for 7 days and its persistence in the different colons was studied until its disappearance from the system. The concentration of short chain fatty acids in the colons showed good colonization of the bioreactors by the microbiota and no significant effect related to the phage treatment. Diversity (α and β), the relative abundance of bacteria, and qPCR analysis targeting different genera of interest showed no significant variation following phage administration. Even if further in vitro studies are needed to assess the efficacy of this phage against its bacterial host within the human intestinal ecosystem, the phage ULIP33 exerted no significant change on the global colonic microbiota.
Causes of HIV Treatment Interruption during the Last 20 Years: A Multi-Cohort Real-Life Study
Authors: De Vito Andrea , Ricci Elena , Menzaghi Barbara , Orofino Giancarlo , Martinelli Canio Vito , Squillace Nicola , Taramasso Lucia , De Socio Giuseppe Vittorio , Molteni Chiara , Valsecchi Laura , Costa Cecilia , Celesia Benedetto Maurizio , Parruti Giustino , Pellicanò Giovanni Francesco , Sarchi Eleonora , Cascio Antonio , Cenderello Giovanni , Falasca Katia , Di Biagio Antonio , Bonfanti Paolo , Madeddu Giordano
Published: March 2023

In the last years, many antiretroviral drugs (ART) have been developed with increased efficacy. Nowadays, the main reasons for treatment switches are adverse events, proactive strategy or simplification. We conducted a retrospective cohort study to investigate the reason for treatment interruption in the last 20 years. We merged data of eight cohorts of the SCOLTA project: lopinavir/r (LPV), atazanavir/r (ATV), darunavir/r or /c (DRV), rilpivirine (RPV), raltegravir (RAL), elvitegravir/c (EVG), dolutegravir (DTG) and bictegravir (BIC). We included 4405 people with HIV (PWH). Overall, 664 (15.1%), 489 (11.1%), and 271 (6.2%) PWH interrupted the treatment in the first, second, and third years after starting a new ART. Looking at the interruption in the first year, the most frequent causes were adverse events (3.8%), loss to follow-up (3.7%), patients’ decisions (2.6%), treatment failure (1.7%), and simplification (1.3%). In the multivariate analysis regarding experienced patients, treatment with LPV, ATV, RPV or EVG/c, having less than 250 CD4 cells/mL, history of intravenous drug use, and HCV positivity were associated with an increased risk of interruption. In naive people, only LPV/r was associated with an increased risk of interruption, while RPV was associated with a lower risk. In conclusion, our data on more than 4400 PWH show that adverse events have represented the most frequent cause of treatment interruptions in the first year of ART (3.84%). Treatment discontinuations were more frequent during the first year of follow-up and decreased thereafter. First-generation PI in both naïve and experienced PWH, and EVG/c, in experienced PWH, were associated with a higher risk of treatment interruptions.
Equity-Informative Economic Evaluations of Vaccines: A Systematic Literature Review
Authors: Patikorn Chanthawat , Cho Jeong-Yeon , Lambach Philipp , Hutubessy Raymond , Chaiyakunapruk Nathorn
Published: March 2023

The Immunization Agenda 2030 prioritizes the populations without access to vaccines. Health equity has been increasingly incorporated into economic evaluations of vaccines to foster equitable access. Robust and standardized methods are needed to evaluate the health equity impact of vaccination programs to ensure monitoring and effective addressing of inequities. However, methods currently in place vary and potentially affect the application of findings to inform policy decision-making. We performed a systematic review by searching PubMed, Embase, Econlit, and the CEA Registry up to 15 December 2022 to identify equity-informative economic evaluations of vaccines. Twenty-one studies were included that performed health equity impact analysis to estimate the distributional impact of vaccines, such as deaths averted and financial risk protection, across equity-relevant subgroups. These studies showed that the introduction of vaccines or improved vaccination coverage resulted in fewer deaths and higher financial risk benefits in subpopulations with higher disease burdens and lower vaccination coverage—particularly poorer income groups and those living in rural areas. In conclusion, methods to incorporate equity have been evolving progressively. Vaccination programs can enhance equity if their design and implementation address existing inequities in order to provide equitable vaccination coverage and achieve health equity.
Evaluation of Feline Permanent Canine Tooth Mineral Density Using Micro-Computed Tomography
Authors: Silva Graça , Babo Pedro S. , Azevedo Jorge , Gomes Manuela E. , Viegas Carlos , Requicha João F.
Published: March 2023

The tooth is made up of three mineralized tissues, enamel, dentin, and cementum, which surround a non-mineralized tissue called the dental pulp. Micro-computed tomography (mCT) is an imaging technology based on X-rays that allows non-invasive visualization of objects at a microscopic scale, according to their radiopacity and in three dimensions (3D). Likewise, it allows the subsequent execution of morphological and quantitative analysis of the objects, such as, for example, the determination of the relative mineral density (MD). The present work aimed to describe the MD of feline teeth using mCT. The studied sample consisted of four European Shorthair cats, from which nine canine teeth were extracted per medical indication. These teeth were evaluated through dental radiography before and after their extraction. Using mCT and the CTAn software, the values of the relative mineral density of the root of each tooth and of specific segments corresponding to the coronal, middle, and apical thirds of the root were determined. Mean MD of root tissues was 1.374 ± 0040 g·cm−3, and of hard root, tissues was 1.402 ± 0.035 g·cm−3. Through mCT, it was possible to determine the mean MD values of feline canine teeth. The study of MD could become an ancillary method for the diagnosis and characterization of dental pathology.
Simulation of the Human Myopic Eye Cornea Compensation Based on the Analysis of Aberrometric Data
Authors: Khorin Pavel A. , Khonina Svetlana N.
Published: March 2023

Various diffractive, refractive and holographic optical elements, such as diffraction gratings; microlens raster; phase plates; multi-order diffractive optical elements; adaptive mirrors; diffractive and refractive axicons; holographic multiplexes and many others are used to analyze wavefront aberrations. We shortly discuss the features (advantages and disadvantages) of various wavefront aberration sensors in the Introduction. The main part of the paper is devoted to the analysis of the weight coefficients of Zernike polynomials obtained during medical examinations of the cornea in the human eye. Using data obtained by aberrometers, the average values of the Zernike polynomial coefficients for the anterior and posterior surfaces of the healthy eye cornea and a myopic one were calculated. The original wavefront for the anterior and posterior surfaces of the cornea was restored separately, as well as the total wave aberration. For an objective assessment of the quality of vision, the corresponding point spread functions (PSFs) were calculated. We propose to compensate for the aberrations of the myopic eye, taking into account the physical features of the corneal surface. The results of numerical simulation showed that in order to improve the quality of the patient’s vision, it is necessary to take into account high-order aberrations of the anterior surface of the cornea in the form of a coma of the third order and aberrations of the fourth order.
Research on Liquid Flow Measurement Method Based on Heat Transfer Method
Authors: Qin Hongwei , Dang Ruirong , Dang Bo
Published: March 2023

Thermal flowmeters are used more and more widely in liquid flow measurement. In this paper, the mechanical shape of the thermal flowmeter is designed, and the optimal installation position of the thermal probe is determined. In the aspect of measurement mechanism research, three heating methods of the thermal probe are deduced: constant voltage heating method, constant current heating method, and constant power heating method. After reasoning, the constant current heating method is determined to be ideal, so the constant current heating method is selected to heat the speed-measuring probe in the experiment. By analyzing the power factor of convection heat transfer and residual heat source of the heating probe, it is concluded that the measurement range of the thermal flowmeter is 0.5–15 m3/d, the flow in this range is proportional to the electrical signal, and the relative error of measurement is within ±5.8%. According to the analysis of the experimental results, the thermal flowmeter has a simple mechanical structure and no redundant moving parts, which can prolong its service life when used on site. When considering industrial applications, the error may be greater than the laboratory error.
Integrated GIS, Remote Sensing, and Electrical Resistivity Tomography Methods for the Delineation of Groundwater Potential Zones in Sangaw Sub-Basin, Sulaymaniyah, KRG-Iraq
Authors: Sangawi Azad , Al-Manmi Diary Ali Mohammed , Aziz Bakhtiar Qader
Published: March 2023

In the Sangaw region, groundwater is the primary supply of water for drinking, residential purposes, livestock, and summer farming activities. Therefore, the main objective of this research is to delineate groundwater potential zones (GWPZs) in the Sangaw sub-basin, Sulaymaniyah, KRG-Iraq, by integrating geographic information system (GIS), remote sensing (RS), analytical hierarchy process (AHP), and electrical resistivity tomography (ERT) techniques. Seven different thematic layers, including geology, rainfall, slope, lineament density, land use/land cover, drainage density, and topographic position index, were chosen as the prediction factors. The analytical method of an analytical hierarchy process pair-wise matrix was used to evaluate the normalized weight of these thematic layers. All the layers and their corresponding classes were assigned ranks and weights based on their impact on groundwater potential. Using ArcGIS, these thematic maps were combined to precisely determine the groundwater potential map within the research area. Five different potential zones were generated for the resulting map, namely, very low (55.4 km2), low (90.4 km2), moderate (68.1 km2), high (100 km2), and very high (62.4 km2). The findings revealed that almost 43.2% of the study region is characterized by high to very high groundwater potential zones. In contrast, the very low to low groundwater potential covers around 38.7%, and the moderate groundwater potential occupies 18.1% of the study region. The final map was then validated using results from the two-dimensional inverse sections of eight electrical resistivity tomography profiles. The validation data confirmed that groundwater potential classes strongly overlap with the subsurface water-bearing or non-bearing lithology, and groundwater productivity zones in the given area. The novelty of this research lies in the application of electrical resistivity tomography validation to the groundwater potential mapping approach, which illustrates the robustness of the overall methodology for data-scarce areas. Furthermore, this is one of the very few groundwater potential studies in Iraq and the first in the Sangaw sub-basin, which can assist decision-makers with groundwater prospecting and management, and enable further exploration in the region.
Correction: Islam, M.A.; Sato, T. Influence of Terrestrial Precipitation on the Variability of Extreme Sea Levels along the Coast of Bangladesh. Water 2021, 13, 2915
Authors: Islam Md. Anowarul , Sato Tomonori
Published: March 2023

In the original publication [...]