bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual-Slope Diffuse Reflectance Instrument for Calibration-Free Broadband Spectroscopy
Authors: Blaney Giles , Donaldson Ryan , Mushtak Samee , Nguyen Han , Vignale Lydia , Fernandez Cristianne , Pham Thao , Sassaroli Angelo , Fantini Sergio
Published: February 2021

This work presents the design and validation of an instrument for dual-slope broadband diffuse reflectance spectroscopy. This instrument affords calibration-free, continuous-wave measurements of broadband absorbance of optically diffusive media, which may be translated into absolute absorption spectra by adding frequency-domain measurements of scattering at two wavelengths. An experiment on a strongly scattering liquid phantom (milk, water, dyes) confirms the instrument’s ability to correctly identify spectral features and measure absolute absorption. This is done by sequentially adding three dyes, each featuring a distinct spectral absorption, to the milk/water phantom. After each dye addition, the absorption spectrum is measured, and it is found to reproduce the spectral features of the added dye. Additionally, the absorption spectrum is compared to the absorption values measured with a commercial frequency-domain instrument at two wavelengths. The measured absorption of the milk/water phantom quantitatively agrees with the known water absorption spectrum (R2=0.98), and the measured absorption of the milk/water/dyes phantom quantitatively agrees with the absorption measured with the frequency-domain instrument in six of eight cases. Additionally, the measured absorption spectrum correctly recovers the concentration of one dye, black India ink, for which we could accurately determine the extinction spectrum (i.e., the specific absorption per unit concentration). The instrumental methods presented in this work can find applications in quantitative spectroscopy of optically diffusive media, and particularly in near-infrared spectroscopy of biological tissue.
Stability Evaluation of TBM Pilot Tunnels to Rear Blasting Using the Protection Shield
Authors: Kong Suk-Min , Choi Sang-Il , Shim Seung-Bo , Lee Hana , Oh Dong-Wook , Lee Seong-Won
Published: February 2021

Recent studies have increasingly investigated construction methods for tunnel excavation because of growing underground space development. Although the New Austrian tunneling method (NATM)—a representative tunnel excavation method—can be applied to various ground conditions, as well as having good constructability and economic feasibility, it suffers from problems such as vibration and noise. By contrast, excavation using a tunnel boring machine (TBM)—a representative mechanized construction method—affords advantages such as stable excavation, minimized ground and environmental damage, noise, and vibration. However, it cannot be applied easily to various ground conditions, and it suffers from problems such as high construction costs and delays owing to equipment defects. Therefore, the simultaneous pilot tunnel excavation using the TBM—which affords advantages such as constructability, economic feasibility, and minimized noise and vibration—and rear enlargement blasting using NATM was investigated in this study. A protection shield was installed to minimize accidents occurring from equipment defects (a disadvantage of TBM) and analyze the decrease in the effect of NATM blasting, which depends on the use of the protection shield and the separation distance through 3D numerical analysis.
The Impact of Hydrogeological Features on the Performance of Underground Pumped-Storage Hydropower (UPSH)
Authors: Pujades Estanislao , Poulain Angelique , Orban Philippe , Goderniaux Pascal , Dassargues Alain
Published: February 2021

Underground pumped storage hydropower (UPSH) is an attractive opportunity to manage the production of electricity from renewable energy sources in flat regions, which will contribute to the expansion of their use and, thus, to mitigating the emissions of greenhouse gasses (GHGs) in the atmosphere. A logical option to construct future UPSH plants consists of taking advantage of existing underground cavities excavated with mining purposes. However, mines are not waterproofed, and there will be an underground water exchange between the surrounding geological medium and the UPSH plants, which can impact their efficiency and the quality of nearby water bodies. Underground water exchanges depend on hydrogeological features, such as the hydrogeological properties and the groundwater characteristics and behavior. In this paper, we numerically investigated how the hydraulic conductivity (K) of the surrounding underground medium and the elevation of the piezometric head determined the underground water exchanges and their associated consequences. The results indicated that the efficiency and environmental impacts on surface water bodies became worse in transmissive geological media with a high elevation of the piezometric head. However, the expected environmental impacts on the underground medium increased as the piezometric head became deeper. This assessment complements previous ones developed in the same field and contributes to the definition of (1) screening strategies for selecting the best places to construct future UPSH plants and (2) design criteria to improve their efficiency and minimize their impacts.
Machine-Learning-Based Elderly Stroke Monitoring System Using Electroencephalography Vital Signals
Authors: Choi Yoon-A , Park Sejin , Jun Jong-Arm , Ho Chee Meng Benjamin , Pyo Cheol-Sig , Lee Hansung , Yu Jaehak
Published: February 2021

Stroke is the third highest cause of death worldwide after cancer and heart disease, and the number of stroke diseases due to aging is set to at least triple by 2030. As the top three causes of death worldwide are all related to chronic disease, the importance of healthcare is increasing even more. Models that can predict real-time health conditions and diseases using various healthcare services are attracting increasing attention. Most diagnosis and prediction methods of stroke for the elderly involve imaging techniques such as magnetic resonance imaging (MRI). It is difficult to rapidly and accurately diagnose and predict stroke diseases due to the long testing times and high costs associated with MRI. Thus, in this paper, we design and implement a health monitoring system that can predict the precursors of stroke diseases in the elderly in real time during daily walking. First, raw electroencephalography (EEG) data from six channels were preprocessed via Fast Fourier Transform (FFT). The raw EEG power values were then extracted from the raw spectra: alpha (α), beta (β), gamma (γ), delta (δ), and theta (θ) as well as the low β, high β, and θ to β ratio, respectively. The experiments in this paper confirm that the important features of EEG biometric signals alone during walking can accurately determine stroke precursors and occurrence in the elderly with more than 90% accuracy. Further, the Random Forest algorithm with quartiles and Z-score normalization validates the clinical significance and performance of the system proposed in this paper with a 92.51% stroke prediction accuracy. The proposed system can be implemented at a low cost, and it can be applied for early disease detection and prediction using the precursor symptoms of real-time stroke. Furthermore, it is expected that it will be able to detect other diseases such as cancer and heart disease in the future.
Roles of Phosphate Solubilizing Microorganisms from Managing Soil Phosphorus Deficiency to Mediating Biogeochemical P Cycle
Authors: Tian Jiang , Ge Fei , Zhang Dayi , Deng Songqiang , Liu Xingwang
Published: February 2021

Phosphorus (P) is a vital element in biological molecules, and one of the main limiting elements for biomass production as plant-available P represents only a small fraction of total soil P. Increasing global food demand and modern agricultural consumption of P fertilizers could lead to excessive inputs of inorganic P in intensively managed croplands, consequently rising P losses and ongoing eutrophication of surface waters. Despite phosphate solubilizing microorganisms (PSMs) are widely accepted as eco-friendly P fertilizers for increasing agricultural productivity, a comprehensive and deeper understanding of the role of PSMs in P geochemical processes for managing P deficiency has received inadequate attention. In this review, we summarize the basic P forms and their geochemical and biological cycles in soil systems, how PSMs mediate soil P biogeochemical cycles, and the metabolic and enzymatic mechanisms behind these processes. We also highlight the important roles of PSMs in the biogeochemical P cycle and provide perspectives on several environmental issues to prioritize in future PSM applications.
IgLON5 Regulates the Adhesion and Differentiation of Myoblasts
Authors: Lim Jeong Ho , Beg Mirza Masroor Ali , Ahmad Khurshid , Shaikh Sibhghatulla , Ahmad Syed Sayeed , Chun Hee Jin , Choi Dukhwan , Lee Woo-Jong , Jin Jun-O , Kim Jihoe , Jan Arif Tasleem , Lee Eun Ju , Choi Inho
Published: February 2021

IgLON5 is a cell adhesion protein belonging to the immunoglobulin superfamily and has important cellular functions. The objective of this study was to determine the role played by IgLON5 during myogenesis. We found IgLON5 expression progressively increased in C2C12 myoblasts during transition from the adhesion to differentiation stage. IgLON5 knockdown (IgLON5kd) cells exhibited reduced cell adhesion, myotube formation, and maturation and reduced expressions of different types of genes, including those coding for extracellular matrix (ECM) components (COL1a1, FMOD, DPT, THBS1), cell membrane proteins (ITM2a, CDH15), and cytoskeletal protein (WASP). Furthermore, decreased IgLON5 expression in FMODkd, DPTkd, COL1a1kd, and ITM2akd cells suggested that IgLON5 and these genes mutually control gene expression during myogenesis. IgLON5 immunoneutralization resulted in significant reduction in the protein level of myogenic markers (MYOD, MYOG, MYL2). IgLON5 expression was higher in the CTX-treated gastrocnemius mice muscles (day 7), which confirmed increase expression of IgLON5 during muscle. Collectively, these results suggest IgLON5 plays an important role in myogenesis, muscle regeneration, and that proteins in ECM and myoblast membranes form an interactive network that establishes an essential microenvironment that ensures muscle stem cell survival.
Cusp of Non-Gaussian Density of Particles for a Diffusing Diffusivity Model
Authors: Hidalgo-Soria M. , Barkai E. , Burov S.
Published: February 2021

We study a two state “jumping diffusivity” model for a Brownian process alternating between two different diffusion constants, D+>D−, with random waiting times in both states whose distribution is rather general. In the limit of long measurement times, Gaussian behavior with an effective diffusion coefficient is recovered. We show that, for equilibrium initial conditions and when the limit of the diffusion coefficient D−⟶0 is taken, the short time behavior leads to a cusp, namely a non-analytical behavior, in the distribution of the displacements P(x,t) for x⟶0. Visually this cusp, or tent-like shape, resembles similar behavior found in many experiments of diffusing particles in disordered environments, such as glassy systems and intracellular media. This general result depends only on the existence of finite mean values of the waiting times at the different states of the model. Gaussian statistics in the long time limit is achieved due to ergodicity and convergence of the distribution of the temporal occupation fraction in state D+ to a δ-function. The short time behavior of the same quantity converges to a uniform distribution, which leads to the non-analyticity in P(x,t). We demonstrate how super-statistical framework is a zeroth order short time expansion of P(x,t), in the number of transitions, that does not yield the cusp like shape. The latter, considered as the key feature of experiments in the field, is found with the first correction in perturbation theory.
Cylindrical Waveguide on Ferrite Substrate Controlled by Externally Applied Magnetic Field
Authors: Sakli Hedi
Published: February 2021

This paper presents an extension of the formulation of wave propagation in transverse electric (TE) and transverse magnetic (TM) modes for the case of metallic cylindrical waveguides filled with longitudinally magnetized ferrite. The higher order modes were exploited. We externally controlled the cut-off frequency through the application of DC magnetic fields. The numerical results of dispersion diagrams for TE and TM modes were obtained and analyzed. We analyzed a waveguide antenna filled with partially magnetized ferrite using the mode matching (MM) technique based on the TE and TM modes. By using modal analysis, our approach considerably reduced the computation time compared to HFSS. Ferrites are important for various industrial applications, such as circulators, isolators, antennas and filters.
Crashed Electric Vehicle Handling and Recommendations—State of the Art in Germany
Authors: Wöhrl Katharina , Geisbauer Christian , Nebl Christoph , Lott Susanne , Schweiger Hans-Georg
Published: February 2021

In the near future, electric powered vehicles will represent a major part of the road traffic. Accordingly, there will be a natural increase of accidents involving electric vehicles. There are not many cases of such accidents yet and therefore the experience and correct handling are still partially open points for the involved parties, such as the rescue services for example. The aim of this work is to provide a complete overview of the accident handling sequence in Germany, starting with the damaged vehicle on site and moving on to the risks and challenges for the stakeholders, such as transport and recycling companies. Arising from the developed overview, a handling recommendation for yet undiscussed points is given. Especially, different extinguishing and deactivation methods are compared and discussed. Due to a lack of a common live-feed from battery data on site, other criteria have to be taken into account to assess the state of the battery. The wrecked vehicle—including the high voltage system—needs to be in a definite safe state at the handover to a towing service. Depending on the case, different options for securing the vehicle will be considered in this work.
Visual Comfort Analysis of Semi-Transparent Perovskite Based Building Integrated Photovoltaic Window for Hot Desert Climate (Riyadh, Saudi Arabia)
Authors: Ghosh Aritra , Mesloub Abdelhakim , Touahmia Mabrouk , Ajmi Meriem
Published: February 2021

Buildings consume considerable amount of energy to maintain comfortable interior. By allowing daylight, visual comfort inside a building is possible which can enhance the occupant’s health, mood and cognitive performance. However, traditional highly transparent windows should be replaced with semitransparent type window to attain a comfortable daylight inside a building. Evaluation of visual comfort includes both daylight glare and colour comfort analysis. Building integrated photovoltaic (BIPV) type windows are promising systems and can possess a range of semitransparent levels depending on the type of PV used. In this work, the semitransparent Perovskite BIPV windows was investigated by employing daylight glare analysis for an office building located in Riyadh, KSA and three wavelength dependent transmission spectra for colour comfort analysis. The results showed that the transmissions range between 50–70% was optimum for the comfortable daylight for south facing vertical pane BPV-windows. However, excellent colour comfort was attained for the transmission range of 90% which provided glare issues. Colour comfort for 20% transparent Perovskite was compared with contemporary other type of PV which clearly indicated that wavelength dependent transmittance is stronger over single value transmittance.
Assessing the Techno-Economic Impact of Derating Factors on Optimally Tilted Grid-Tied Photovoltaic Systems
Authors: Masrur Hasan , Konneh Keifa Vamba , Ahmadi Mikaeel , Khan Kaisar R. , Othman Mohammad Lutfi , Senjyu Tomonobu
Published: February 2021

Photovoltaic (PV) systems encounter substantial losses throughout their lifespan due to the different derating factors of PV modules. Those factors mainly vary according to the geographical location and PV panel characteristics. However, the available literature does not explicitly concentrate on the technical and economic impact of the derating factors within the PV system. Owing to that necessity, this study performs a comprehensive analysis of various PV loss parameters followed by a techno-economic assessment of derating factors using the average value on a grid-connected and optimally tilted PV system located in Hatiya, Bangladesh. Some criteria linked to the derating factors such as PV degradation and ambient temperature are further explored to analyze their impact on the aforementioned power system. Simulation results show that PV power generation would vary around 12% annually, subject to a 10% variation in the derating factor. Again, a 10% difference in the derating factor changes the net present cost (NPC) by around 3% to 4%. The system provides the best technical performance concerning annual PV production, power trade with the grid, and the renewable fraction at a higher value of the derating factor since it represents a lower impact of the loss parameters. Similarly, the financial performance in terms of the NPC, levelized cost of energy (LCOE), and grid power exchange cost is found to be lower when the derating factor value is higher.
The Effect of Fermented Kefir as Functional Feed Additive in Post-Weaned Pigs
Authors: Choi Woosik , Son Dang Bao , Hong Jeongpyo , Jeong Dabeen , Kim Hee-Chang , Lee Hanki , Suh Joo-Won
Published: February 2021

The control of the immune system of pigs after weaning is important in pig farming because productivity depends on the survival of the post-weaned pigs. Previously, antibiotics would have been administered in the case of infectious diseases to increase the survival rate of post-weaned pigs, but now, the use of antibiotics is strictly restricted in order to prevent other problems such as the occurrence of antibiotic-resistant pathogens. In this study, the effect of fermented kefir as a functional feed additive as a replacement to antibiotics was evaluated in terms of the microbial profile in fecal samples, immunological factors in the blood of pigs, growth performance measured as average daily gain (ADG) and the feed conversion rate (FCR) of post-weaned pigs. In the kefir-treated group, the number of lactic acid bacteria and Bacillus spp. in the fecal samples of the pigs increased with the kefir treatments. Interestingly, the number of coliform groups as opportunistic pathogens was reduced in the fecal samples of pigs treated with kefir. We found out that treatment with kefir enhanced the innate immunity of post-weaned pigs though the reduction of IL-6 as a proinflammatory cytokine and an increase in IgG as an immunoglobulin, enhancing immunological defense against pathogens. Finally, after treatment with kefir, we observed that the ADG of post-weaned pigs increased to 135.6% but FCR decreased to 92.2%. Therefore, this study shows that fermented kefir can be used as a functional feed additive and an antibiotic alternative in order to improve both the innate immune system and growth performance of post-weaned pigs.
Carotenoid and Carotenoid Ester Profile and Their Deposition in Plastids in Fruits of New Papaya (Carica papaya L.) Varieties from the Canary Islands
Authors: Lara-Abia Sara , Lobo-Rodrigo Gloria , Welti-Chanes Jorge , Cano M. Pilar
Published: February 2021

The carotenoid profile of non-saponified and saponified extracts of different tissues (pulp and peel) of fruits of three new papaya varieties, Sweet Mary, Alicia, and Eksotika, was characterized for the first time, and almost all carotenoid compounds were quantified. Carotenoids and carotenoid esters were analyzed and characterized using HPLC-photo diode array (PDA-MS with atmospheric pressure chemical ionization with positive ion mode (APCI+) with a C30 reversed-phase column. The carotenoid deposition in collenchyma and chlorenchyma cells of papaya pulp and peel tissues was assessed by optical microscopy, confocal laser scanning microscopy, and transmission electron microscopy. The most abundant carotenoids in the fruit of the three papaya varieties (pulp and peel) were (all-E)-lycopene (230.0–421.2 µg/100 g fresh weight), (all-E)-β-carotene (120.3–233.2 µg/100 g fresh weight), and (all-E)-β-cryptoxanthin laurate (74.4–223.2 µg/100 g fresh weight. Moreover, high concentrations of (all-E)-lutein (922.5–1381.1 µg/100 g fresh weight) and its esters, such as (all-E)-lutein-3-O-myristate and (all-E)-lutein dimyristate, were found in peel extracts. The optical microscopy study of papaya pulps showed that carotenoid deposition in all papaya varieties, including Maradol, was mainly localized close to the cell walls, showing the presence of some crystalloids and round-shaped structures, with different sizes and distribution due to the different carotenoid content among varieties. No crystalloids or globular depositions were found in any of the peel sections, and no remarkable differences were found in the papaya peel microstructure of the different papaya varieties.
Discrimination of the Geographical Origin of Soybeans Using NMR-Based Metabolomics
Authors: Zhou Yaoyao , Kim Seok-Young , Lee Jae-Soung , Shin Byeung-Kon , Seo Jeong-Ah , Kim Young-Suk , Lee Do-Yup , Choi Hyung-Kyoon
Published: February 2021

With the increase in soybean trade between countries, the intentional mislabeling of the origin of soybeans has become a serious problem worldwide. In this study, metabolic profiling of soybeans from the Republic of Korea and China was performed by nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate statistical analysis to predict the geographical origin of soybeans. The optimal orthogonal partial least squares-discriminant analysis (OPLS-DA) model was obtained using total area normalization and unit variance (UV) scaling, without applying the variable influences on projection (VIP) cut-off value, resulting in 96.9% sensitivity, 94.4% specificity, and 95.6% accuracy in the leave-one-out cross validation (LOO-CV) test for discriminating between Korean and Chinese soybeans. Soybeans from the northeastern, middle, and southern regions of China were successfully differentiated by standardized area normalization and UV scaling with a VIP cut-off value of 1.0, resulting in 100% sensitivity, 91.7%–100% specificity, and 94.4%–100% accuracy in a LOO-CV test. The methods employed in this study can be used to obtain essential information for the authentication of soybean samples from diverse geographical locations in future studies.
Chronotropic Incompetence Limits Aerobic Exercise Capacity in Patients Taking Beta-Blockers: Real-Life Observation of Consecutive Patients
Authors: Smarz Krzysztof , Tysarowski Maciej , Zaborska Beata , Pilichowska-Paszkiet Ewa , Sikora-Frac Małgorzata , Budaj Andrzej , Jaxa-Chamiec Tomasz
Published: February 2021

Background: Chronotropic incompetence in patients taking beta-blockers is associated with poor prognosis; however, its impact on exercise capacity (EC) remains unclear. Methods: We analyzed data from consecutive patients taking beta-blockers referred for cardiopulmonary exercise testing to assess EC. Chronotropic incompetence was defined as chronotropic index (CI) ≤ 62%. Results: Among 140 patients all taking beta-blockers (age 61 ± 9.7 years; 73% males), 64% with heart failure, chronotropic incompetence was present in 80.7%. EC assessed as peak oxygen uptake was lower in the group with chronotropic incompetence, 18.3 ± 5.7 vs. 24.0 ± 5.3 mL/kg/min, p < 0.001. EC correlated positively with CI (β = 0.14, p < 0.001) and male gender (β = 5.12, p < 0.001), and negatively with age (β = −0.17, p < 0.001) and presence of heart failure (β = −3.35, p < 0.001). Beta-blocker dose was not associated with EC. Partial correlation attributable to CI accounted for more than one-third of the variance in EC explained by the model (adjusted R2 = 59.8%). Conclusions: In patients taking beta-blockers, presence of chronotropic incompetence was associated with lower EC, regardless of the beta-blocker dose. CI accounted for more than one-third of EC variance explained by our model.
Biomechanical Analysis of Serious Neck Injuries Resulting from Judo
Authors: Nakanishi Tomoyuki , Hitosugi Masahito , Murayama Haruo , Takeda Arisa , Motozawa Yasuki , Ogino Masahiro , Koyama Katsuhiro
Published: February 2021

To establish a basis for initial diagnosis and for proposing preventive measures for the serious neck injuries occasionally experienced by judo practitioners, the biomechanical mechanisms of these injuries were analyzed. Two male judo experts repeatedly threw an anthropomorphic test device (POLAR dummy) using three throwing techniques (Seoi-nage, Osoto-gari, and Ouchi-gari). The dummy’s kinematic data were captured using a high-speed digital camera, and the load and moment of the neck were measured with load cells. The neck injury criterion (Nij) and beam criterion were also calculated. In Seoi-nage, the anterior and parietal regions of the dummy’s head contacted the tatami (judo mat). Subsequently, most of the body weight was applied, with the neck experiencing the highest compression. However, in Osoto-gari and Ouchi-gari, the occipital region of the dummy’s head contacted the tatami. Significantly higher values of both Nij (median 0.68) and beam criterion (median 0.90) corresponding to a 34.7% to 37.1% risk of neck injury with an abbreviated injury scale score ≥2 were shown in Seoi-nage than in either Ouchi-gari or Osoto-gari. In judo, when thrown by the Seoi-nage technique, serious neck injuries can occur as a result of neck compression that occurs when the head contacts the ground.
Three Cases of Severe ME/CFS in Adults
Authors: Williams Leah R. , Isaacson-Barash Carol
Published: February 2021

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex, only partially understood multi-system disease whose onset and severity vary widely. Symptoms include overwhelming fatigue, post-exertional malaise, sleep disruptions, gastrointestinal issues, headaches, orthostatic intolerance, cognitive impairment, etc. ME/CFS is a physiological disease with an onset often triggered by a viral or bacterial infection, and sometimes by toxins. Some patients have a mild case and are able to function nearly on a par with healthy individuals, while others are moderately ill and still others are severely, or even, very severely ill. The cohort of moderately to very severely ill is often housebound or bedbound, has lost employment or career, and has engaged in a long, and often futile, search for treatment and relief. Here, we present three case studies, one each of a moderately ill, a severely ill, and a very severely ill person, to demonstrate the complexity of the disease, the suffering of these patients, and what health care providers can do to help.
Work-Related Challenges among Primary Health Centers Workers during COVID-19 in Saudi Arabia
Authors: Al-Mansour Khalid , Alfuzan Abdullatif , Alsarheed Danya , Alenezi Munwer , Abogazalah Fouad
Published: February 2021

This study aimed to identify certain occupational risk factors for stress among healthcare workers (HCWs) during the COVID-19 pandemic. Using a multistage random sampling approach, an online questionnaire was applied to collect data on role conflict and ambiguity, self-esteem and social support from 1378 HCWs working in primary health centers (regular and fever clinics; clinics specialized in managing patients with COVID-19 symptoms) across Saudi Arabia. The results showed that stress correlated positively with role conflict and ambiguity and negatively with social support. HCWs in fever clinics exhibited significantly more stress and role conflict and ambiguity than those who were working in regular primary healthcare centers. In conclusion, role conflict and ambiguity and social support were determinants for stress among HCWs, especially those working in fever clinics.
A Systematic Review of Self-Report Instruments for the Measurement of Anxiety in Hospitalized Children with Cancer
Authors: Mahakwe Gomolemo , Johnson Ensa , Karlsson Katarina , Nilsson Stefan
Published: February 2021

Anxiety has been identified as one of the most severe and long-lasting symptoms experienced by hospitalized children with cancer. Self-reports are especially important for documenting emotional and abstract concepts, such as anxiety. Children may not always be able to communicate their symptoms due to language difficulties, a lack of developmental language skills, or the severity of their illness. Instruments with sufficient psychometric quality and pictorial support may address this communication challenge. The purpose of this review was to systematically search the published literature and identify validated and reliable self-report instruments available for children aged 5–18 years to use in the assessment of their anxiety to ensure they receive appropriate anxiety-relief intervention in hospital. What validated self-report instruments can children with cancer use to self-report anxiety in the hospital setting? Which of these instruments offer pictorial support? Eight instruments were identified, but most of the instruments lacked pictorial support. The Visual Analogue Scale (VAS) and Pediatric Quality of Life (PedsQL™) 3.0 Brain Tumor Module and Cancer Module proved to be useful in hospitalized children with cancer, as they provide pictorial support. It is recommended that faces or symbols be used along with the VAS, as pictures are easily understood by younger children. Future studies could include the adaptation of existing instruments in digital e-health tools.
Household Vulnerability to Food Insecurity in Rural South Africa: Evidence from a Nationally Representative Survey Data
Authors: Mthethwa Sandile , Wale Edilegnaw
Published: February 2021

Using a nationally representative dataset from rural areas in South Africa, the study examines vulnerability to food insecurity using the Vulnerability as Expected Poverty framework. The dataset used was large and comprehensive to develop robust profiles of vulnerable households. This is executed employing the sustainable livelihoods framework. The findings show that human and financial capital plays a critical role in making rural households resilient from vulnerability to food insecurity. The failure of natural resources to support agricultural livelihoods emerged as an important factor for rural household vulnerability to food insecurity. Gender-based imbalances still prevail, explaining most of the rural household vulnerability to food insecurity. Female-dominated households still endure most of the prevailing vulnerabilities to food insecurity, and this is even worse for households headed by younger females. Policies, strategies, and institutions in South Africa have not been able to address household vulnerability to food insecurity. The study identified Eastern Cape and KwaZulu-Natal as the most vulnerable provinces where food policy has to be a top priority agenda.
Environmental Compliance and Enterprise Innovation: Empirical Evidence from Chinese Manufacturing Enterprises
Authors: Liu Meng , Liu Yun , Zhao Yongliang
Published: February 2021

This paper embeds environmental compliance factor and compliance cost factor into the M-O monopolistic competition and multi-product firm model to construct a theoretical model applicable to environmental compliance and enterprise innovation. In addition, we also construct a new environmental compliance index. We use the random-effects Tobit model and the double hurdle model to empirically test the micro-data from the Database of China Industrial Enterprises from 1998 to 2013, then we use the Generalized Propensity Score Matching (GPSM) to conduct a robustness test. The robustness conclusion is that environmental compliance has a significant U-shaped relationship with enterprise innovation, which means, environmental compliance will inhibit enterprise innovation on the left of the inflection point of environmental compliance (0.669), while environmental compliance on the right of the inflection point will promote enterprise innovation. The sub-sample regressions show that, enhanced environmental compliance of state-owned enterprises, mature enterprises, core area enterprises and export enterprises with low level of the environmental compliance, makes the greater inhibition to enterprise innovation, and enhanced environmental compliance of above enterprises with high level of the environmental compliance, makes the greater contribution to enterprise innovation. To this end, the government should adopt the policy, from the shallower to the deeper, to promote the construction of environmental compliance, and identify the inflection point of the environmental compliance on enterprise innovation to stimulate the role of environmental compliance in promoting enterprise innovation.
How a Depressive Medical Doctor Profited in the Long-Term from a New and Short Psychological Group-Treatment against Major Depressive Disorder
Authors: Niedermoser Daryl Wayne , Kalak Nadeem , Meyer Martin , Schweinfurth Nina , Walter Marc , E. Lang Undine
Published: February 2021

Background: Individuals suffering from major depressive disorder (MDD) often describe workplace-related stress as one of the main causes of their disorder. Here, we present the story of a 33 year old “Bob” (a pseudonym) who suffered from a moderate (Hamilton-21 = 18) major depressive episode. Workplace-related stress seemed to be the main stressor for Bob at the time. We were interested in long-lasting effects of a newly established group called “work-related interpersonal Psychotherapy, W-IPT”. W-IPT consists of eight weekly 90 min sessions. The follow-ups were 12 weeks after the group-treatment and 18 months later. Bob was chosen because he agreed in advance to participate in a follow-up. We were interested if the group-treatment of W-IPT also has a persistent positive effect. Case presentation: We present the case of a 33-year-old man “Bob”. He was included in our previous published pilot-study 2020 with diagnosed moderate MDD, and he attended the group treatment. This case report focuses on a follow-up period of 18 months. A structured clinical interview for DSM-IV was carried out in order to be included in the study, and no comorbid diagnoses were detected. Conclusions: However, the psychotherapeutic effects in this case seem enduring and prolonged. Of course, additional research to study the long-term effects of W-IPT is needed, and more patients need to be included.
A Perspective of the Cumulative Risks from Climate Change on Mt. Everest: Findings from the 2019 Expedition
Authors: Miner Kimberley R. , Mayewski Paul Andrew , Hubbard Mary , Broad Kenny , Clifford Heather , Napper Imogen , Gajurel Ananta , Jaskolski Corey , Li Wei , Potocki Mariusz , Priscu John
Published: February 2021

In 2019, the National Geographic and Rolex Perpetual Planet Everest expedition successfully retrieved the greatest diversity of scientific data ever from the mountain. The confluence of geologic, hydrologic, chemical and microbial hazards emergent as climate change increases glacier melt is significant. We review the findings of increased opportunity for landslides, water pollution, human waste contamination and earthquake events. Further monitoring and policy are needed to ensure the safety of residents, future climbers, and trekkers in the Mt. Everest watershed.
Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β
Authors: Tongkao-on Wannit , Yang Chen , McCarthy Bianca Y. , De Silva Warusavithana G. Manori , Rybchyn Mark S. , Gordon-Thomson Clare , Dixon Katie M. , Halliday Gary M. , Reeve Vivienne E. , Mason Rebecca S.
Published: February 2021

Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β−/−) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D), applied topically protects against the ultraviolet radiation (UV) induction of cutaneous cyclobutane pyrimidine dimers (CPDs) and the suppression of contact hypersensitivity (CHS) in female mice. Here, we compare these responses in female versus male Skh:hr1 mice, in ER-β−/−/−− versus wild-type C57BL/6 mice, and in female ER-blockaded Skh:hr1 mice. The induction of CPDs was significantly greater in male than female Skh:hr1 mice and was more effectively reduced by 1,25(OH)2D in female Skh:hr1 and C57BL/6 mice than in male Skh:hr1 or ER-β−/− mice, respectively. This correlated with the reduced sunburn inflammation due to 1,25(OH)2D in female but not male Skh:hr1 mice. Furthermore, although 1,25(OH)2D alone dose-dependently suppressed basal CHS responses in male Skh:hr1 and ER-β−/− mice, UV-induced immunosuppression was universally observed. In female Skh:hr1 and C57BL/6 mice, the immunosuppression was decreased by 1,25(OH)2D dose-dependently, but not in male Skh:hr1, ER-β−/−, or ER-blockaded mice. These results reveal a sex bias in genetic, inflammatory, and immune photoprotection by 1,25(OH)2D favoring female mice that is dependent on the presence of ER-β.
Lysophosphatidic Acid Induces Aerobic Glycolysis, Lipogenesis, and Increased Amino Acid Uptake in BV-2 Microglia
Authors: Joshi Lisha , Plastira Ioanna , Bernhart Eva , Reicher Helga , Koyani Chintan N. , Madl Tobias , Madreiter-Sokolowski Corina , Koshenov Zhanat , Graier Wolfgang F. , Hallström Seth , Sattler Wolfgang
Published: February 2021

Lysophosphatidic acid (LPA) species are a family of bioactive lipids that transmit signals via six cognate G protein-coupled receptors, which are required for brain development and function of the nervous system. LPA affects the function of all cell types in the brain and can display beneficial or detrimental effects on microglia function. During earlier studies we reported that LPA treatment of microglia induces polarization towards a neurotoxic phenotype. In the present study we investigated whether these alterations are accompanied by the induction of a specific immunometabolic phenotype in LPA-treated BV-2 microglia. In response to LPA (1 µM) we observed slightly decreased mitochondrial respiration, increased lactate secretion and reduced ATP/ADP ratios indicating a switch towards aerobic glycolysis. Pathway analyses demonstrated induction of the Akt-mTOR-Hif1α axis under normoxic conditions. LPA treatment resulted in dephosphorylation of AMP-activated kinase, de-repression of acetyl-CoA-carboxylase and increased fatty acid content in the phospholipid and triacylglycerol fraction of BV-2 microglia lipid extracts, indicating de novo lipogenesis. LPA led to increased intracellular amino acid content at one or more time points. Finally, we observed LPA-dependent generation of reactive oxygen species (ROS), phosphorylation of nuclear factor erythroid 2–related factor 2 (Nrf2), upregulated protein expression of the Nrf2 target regulatory subunit of glutamate-cysteine ligase and increased glutathione synthesis. Our observations suggest that LPA, as a bioactive lipid, induces subtle alterations of the immunometabolic program in BV-2 microglia.
Transposable Elements and Stress in Vertebrates: An Overview
Authors: Pappalardo Anna Maria , Ferrito Venera , Biscotti Maria Assunta , Canapa Adriana , Capriglione Teresa
Published: February 2021

Since their identification as genomic regulatory elements, Transposable Elements (TEs) were considered, at first, molecular parasites and later as an important source of genetic diversity and regulatory innovations. In vertebrates in particular, TEs have been recognized as playing an important role in major evolutionary transitions and biodiversity. Moreover, in the last decade, a significant number of papers has been published highlighting a correlation between TE activity and exposition to environmental stresses and dietary factors. In this review we present an overview of the impact of TEs in vertebrate genomes, report the silencing mechanisms adopted by host genomes to regulate TE activity, and finally we explore the effects of environmental and dietary factor exposures on TE activity in mammals, which is the most studied group among vertebrates. The studies here reported evidence that several factors can induce changes in the epigenetic status of TEs and silencing mechanisms leading to their activation with consequent effects on the host genome. The study of TE can represent a future challenge for research for developing effective markers able to detect precocious epigenetic changes and prevent human diseases.
Aphid Assemblages Associated with Urban Park Plant Communities
Authors: Barczak Tadeusz , Bennewicz Janina , Korczyński Maciej , Błażejewicz-Zawadzińska Małgorzata , Piekarska-Boniecka Hanna
Published: February 2021

Some aphid species are important agricultural pests, sometimes also found on plants growing in urban areas. In this work, we set out to identify the plant species, communities or habitats that are more attractive to aphids in order to limit their spread into new green areas. The aim of the study was to determine and compare plant communities and the assemblages of aphids associated with them in different urban park habitats. The research hypothesis assumed that the differences between aphid assemblages depend on plant diversity and hence reflect urban park habitat environmental conditions, in particular the plant communities and the floral structure. The study was carried out in Bydgoszcz (northern Poland), and four parks were taken into consideration. Herein, floristic lists were used to calculate ecological indicator values for each park. The aphid species richness was determined, as well as the relative abundance and dominance structure similarities of the aphid assemblages. Our results demonstrated that Prunus spp. were strongly infested by Hyalopterus pruni, similarly as Philadelphus inodorus by Aphis fabae, Sambucus nigra by Aphis sambuci, and Acerplatanoides and A. pseudoplatanus by Periphyllus testudinaceus. Park plantations of Robinia pseudoacacia were not very attractive to aphids. The most attractive plant communities to aphids were syntaxonomically identifiable as alluvial alder forests in the layer of trees and Cornus sanguinea in the layer of shrubs.
Adolescents’ Concerns, Routines, Peer Activities, Frustration, and Optimism in the Time of COVID-19 Confinement in Spain
Authors: Muñoz-Fernández Noelia , Rodríguez-Meirinhos Ana
Published: February 2021

The global outbreak of COVID-19 has brought changes in adolescents’ daily routines, restrictions to in-person interactions, and serious concerns about the situation. The purpose of this study was to explore COVID-19-related concerns, daily routines, and online peer activities during the confinement period according to sex and age groups. Additionally, the relationship of these factors and optimism along with adolescents’ frustration was examined. Participants included 1246 Spanish students aged 16–25 years old (M = 19.57; SD = 2.53; 70.8% girls). The results indicated that the top concern was their studies. COVID-19-related concerns, daily routines, and online peer activities varied by sex and age. Findings also revealed moderate to high levels of frustration, which were associated with adolescents’ main concerns, online peer activities, maintaining routines, and optimism. The results are discussed in light of their implications in designing support programs and resources to reduce the psychological impact of COVID-19 on adolescent mental health.
Proteinuria Is Associated with the Development of Crohn’s Disease: A Nationwide Population-Based Study
Authors: Park Seona , Lee Hyun Jung , Han Kyung-Do , Soh Hosim , Moon Jung Min , Hong Seung Wook , Kang Eun Ae , Im Jong Pil , Kim Joo Sung
Published: February 2021

Background and Aims: The impact of proteinuria and its severity on the incidence of inflammatory bowel disease (IBD) has not yet been studied. We aimed to determine the association between proteinuria measured by urine dipstick tests and the development of IBD. Methods: This nationwide population-based study was conducted using the Korean National Health Insurance Service (NHIS) database. A total of 9,917,400 people aged 20 years or older who had undergone a national health examination conducted by the NHIS in 2009 were followed up until 2017. The study population was classified into four groups—negative, trace, 1+, and ≥ 2+—according to the degree of proteinuria measured by the urine dipstick test. The primary endpoint was newly diagnosed IBD, Crohn’s disease (CD), or ulcerative colitis (UC) during the follow-up period. Results: Compared with the dipstick-negative group, the incidence of CD significantly increased according to the degree of proteinuria (adjusted hazard ratio [aHR] with 95% confidence interval [CI], 1.01 [0.703–1.451], 1.515 [1.058–2.162], and 2.053 [1.301–3.24] in the trace, 1+, and ≥ 2+ dipstick groups, respectively; p for trend 0.007). However, there was no significant difference in the incidence of UC according to the degree of proteinuria (aHR with 95% CI, 1.12 [0.949–1.323], 0.947 [0.764–1.174], and 1.009 [0.741–1.373] in the trace, 1+, and ≥ 2+ dipstick groups, respectively; p for trend 0.722). In the subgroup analysis, dipstick-positive proteinuria independently increased the incidence of CD regardless of the subgroup. However, dipstick-positive proteinuria was associated with the risk of UC in those with diabetes mellitus and not in those without diabetes mellitus (aHR, 1.527 vs. 0.846; interaction p-value 0.004). The risk of CD was increased or decreased according to proteinuria changes but not associated with the risk of UC. Conclusion: Proteinuria, measured by the dipstick test, is strongly associated with the development of CD.