bluuebunny/crossref_metadata_2025 huggingface dataset:
A non-local continuum poro-damage mechanics model for hydrofracturing of surface crevasses in grounded glaciers
Authors: Duddu Ravindra , Jiménez Stephen , Bassis Jeremy
Published: April 2020

Hydrofracturing can enhance the depth to which crevasses propagate and, in some cases, allow full depth crevasse penetration and iceberg detachment. However, many existing crevasse models either do not fully account for the stress field driving the hydrofracture process and/or treat glacier ice as elastic, neglecting the non-linear viscous rheology. Here, we present a non-local continuum poro-damage mechanics (CPDM) model for hydrofracturing and implement it within a full Stokes finite element formulation. We use the CPDM model to simulate the propagation of water-filled crevasses in idealized grounded glaciers, and compare crevasse depths predicted by this model with those from linear elastic fracture mechanics (LEFM) and zero stress models. We find that the CPDM model is in good agreement with the LEFM model for isolated crevasses and with the zero stress model for closely-spaced crevasses, until the glacier approaches buoyancy. When the glacier approaches buoyancy, we find that the CPDM model does not allow the propagation of water-filled crevasses due to the much smaller size of the tensile stress region concentrated near the crevasse tip. Our study suggests that the combination of non-linear viscous and damage processes in ice near the tip of a water-filled crevasse can alter calving outcomes.
Two new early Asteroidea (Echinodermata) and early asteroid evolution
Authors: Blake Daniel B. , Gahn Forest J. , Guensburg Thomas E.
Published: April 2020

Aerliceaster nexosusn. gen. n. sp. (Echinodermata), one of the oldest of known asteroids, is based on six specimens from the Floian (Early Ordovician) Garden City Formation of Idaho, andKolataster perplexusn. gen. n. sp. is based on two specimens from the Sandian (Late Ordovician) Mifflin Formation of Illinois. Although the asterozoan skeleton is subdivided into few ossicular categories, evolutionary derivations of all the categories are not fully established, and therefore published evaluations differ. Beginning with phylogenetic work placing asteroid ancestry within the Somasteroidea together with the new taxa described herein, aspects of early asteroid morphology are evaluated and ambiguities in need of further study identified. Uncertainties are considered to be founded in rapid early asterozoan diversification and the scanty fossil record.

UUID:http://zoobank.org/b43d07cc-c8fb-4a84-bc6f-40aa6e0daea2
Artificial intelligence to detect tympanic membrane perforations
Authors: Habib A-R , Wong E , Sacks R , Singh N
Published: April 2020

Objective

To explore the feasibility of constructing a proof-of-concept artificial intelligence algorithm to detect tympanic membrane perforations, for future application in under-resourced rural settings.

Methods

A retrospective review was conducted of otoscopic images analysed using transfer learning with Google's Inception-V3 convolutional neural network architecture. The ‘gold standard’ ‘ground truth’ was defined by otolaryngologists. Perforation size was categorised as less than one-third (small), one-third to two-thirds (medium), or more than two-thirds (large) of the total tympanic membrane diameter.

Results

A total of 233 tympanic membrane images were used (183 for training, 50 for testing). The algorithm correctly identified intact and perforated tympanic membranes (overall accuracy = 76.0 per cent, 95 per cent confidence interval = 62.1–86.0 per cent); the area under the curve was 0.867 (95 per cent confidence interval = 0.771–0.963).

Conclusion

A proof-of-concept image-classification artificial intelligence algorithm can be used to detect tympanic membrane perforations and, with further development, may prove to be a valuable tool for ear disease screening. Future endeavours are warranted to develop a point-of-care tool for healthcare workers in areas distant from otolaryngology.
Introduction: Why a History of Cultural Expertise?
Authors: Holden Livia
Published: April 2020

This is an introduction to a scholarly forum that is an outcome of the workshop entitled Cultural Expertise in Ancient and Modern History held in Oxford in July 2018 and aims to make explicit the interdisciplinary components of cultural expertise from a historiographical perspective in order to open up the discussion to the history of law.
Influence of timing of Palmer amaranth control in dicamba-resistant cotton on yield and economic return
Authors: Inman Matthew D. , Jordan David L. , Vann Matthew C. , Hare Andrew T. , York Alan C. , Cahoon Charles W.
Published: April 2020

Glyphosate-resistant (GR) Palmer amaranth continues to be challenging to control across the U.S. cotton belt. Timely application of POST herbicides and herbicides applied at planting or during the season with residual activity are utilized routinely to control this weed. Although glyphosate controls large Palmer amaranth that is not GR, herbicides such as glufosinate used in resistance management programs for GR Palmer amaranth must be applied when weeds are small. Dicamba can complement both glyphosate and glufosinate in controlling GR and glyphosate-susceptible (GS) biotypes in resistant cultivars. Two studies were conducted to determine Palmer amaranth control, weed biomass, and cotton yield, as well as to estimate economic net return when herbicides were applied 2, 3, 4, and 5 wk after planting (WAP). In one experiment POST-only applications were made. In the second experiment PRE herbicides were included. In general, Palmer amaranth was controlled at least 98% by herbicides applied at least three times regardless of timing of application or herbicide sequence. Glyphosate plus dicamba applied at 4 and 5 WAP controlled Palmer amaranth similarly compared to three applications by 8 WAP; however, yield was reduced 23% because of early-season interference. The inclusion of PRE herbicides benefited treatments that did not include herbicides applied 2 or 3 WAP. Glyphosate plus dicamba applied as the only herbicides 5 WAP provided 69% control of Palmer amaranth. PRE herbicides increased control to 96% for this POST treatment. Economic returns were similar when three or more POST applications were applied, with or without PRE herbicides.
Depressed Mood Prior to Death
Authors: Schlagbaum Paige , Ruch Donna A. , Tissue Jaclyn L. , Sheftall Arielle H. , Bridge Jeffrey A.
Published: April 2020

Abstract. Background: Suicide is a leading cause of death among youth in the United States. Multiple factors have been shown to increase risk for suicidal behavior, including depressed mood. Aims: The purpose of this study was to examine individual characteristics and precipitating circumstances of suicide in youth decedents with and without depressed mood at the time of death. Method: Data from the National Violent Death Reporting System (NVDRS) were analyzed for 17 US states from 2003 to 2012. Participants included suicide decedents aged 10–19 years ( N = 4,053). Analyses compared youth suicide decedents with depressed mood at time of death with those without depressed mood using logistic regression. Sex-specific differences in youth with depressed mood were also explored. Results: Youth suicide decedents with depressed mood were more likely than those without depressed mood to exhibit clinical characteristics and precipitating circumstances associated with suicide. Comparison of males and females with depressed mood found unique sex-specific differences. Limitations: Data were limited to 17 states, analyses did not include a control group, and data were collected through postmortem reporting. Conclusion: Findings support a significant association between depressed mood and factors associated with suicidal behavior in youth and offer potential areas to focus prevention strategies.
Validation of the Suicidal Behavior Exposure Scale in First Responders
Authors: Wolford-Clevenger Caitlin , Smith Phillip N. , Kuhlman Shane , Morgan Ronald , Stefurak Tres
Published: April 2020

Abstract. Background: First responders (e.g., emergency medical technicians, firefighters) may be at higher suicide risk than the general population due to frequent exposure to suicidal behaviors of others. Aims: We aimed to confirm the factor structure, scale reliability, and convergent validity of a new measure of suicidal exposure, the Suicidal Behaviors Exposure Scale, in first responders. Method: Using a cross-sectional web-based survey, we recruited 862 participants (81.3% male, mean age = 40.23, SD = 11.88) from a national database and southeastern state-based organization of first responders. Results: Confirmatory factor analysis resulted in a generally poor fit for the three-factor structure (exposure to suicidal communication, indirect exposure to suicide attempts/deaths, direct exposure to suicide attempts/deaths). However, good scale reliability and convergent validity were demonstrated. Limitations: The cross-sectional design, a convenience sample, and the low percentage of women comprise the limitations of this study. Conclusion: The Suicidal Behavior Exposure Scale requires additional development and validation before use in a first-responder sample. Future work should also examine other aspects of reliability and validity, namely, measurement invariance across groups and time.
Global Estimates of Reach‐Level Bankfull River Width Leveraging Big Data Geospatial Analysis
Authors: Lin Peirong , Pan Ming , Allen George H. , de Frasson Renato Prata , Zeng Zhenzhong , Yamazaki Dai , Wood Eric F.
Published: April 2020

Recent progress in remote sensing has snapshotted unprecedented numbers of river planform geometry, providing opportunity to revisit the oversimplified channel shape parameterizations in global hydrologic models. This study leveraged two recent Landsat‐derived global river width databases and created a reach‐level width dataset to measure the validity of model parameterizations at ~1.6 million kilometers of rivers in length. By showing state‐of‐the‐art parameterization schemes only capture 30–40% of the width variance globally, we developed a machine learning (ML) approach surveying 16 environmental covariates, which considerably improved the predictive power (R2 = 0.81 and 0.77 for two testing cases). Beyond the commonly discussed upstream basin conditions, ML revealed that local physiographic factors and human interference are also important covariates for width variability. Finally, we applied the ML model to estimate bankfull river width, creating a new reach‐level dataset for use in global hydrodynamic modeling.
Estimating Southern Ocean Storm Positions With Seismic Observations
Authors: Hell Momme C. , Gille Sarah T. , Cornuelle Bruce D. , Miller Arthur J. , Bromirski Peter D. , Crawford Alex D.
Published: April 2020

Surface winds from Southern Ocean cyclones generate large waves that travel over long distances (>1,000 km). Wave generation regions are often colocated with enhanced air‐sea fluxes and upper ocean mixing. Ocean wave spectra contain information about storm wind speed, fetch size, and intensity at their generation site. Two years of seismic observations on the Ross Ice shelf, combined with modern optimization (machine learning) techniques, are used to trace the origins of wave events in the Southern Ocean with an accuracy of ±110 km and ±2 hr from a hypothetical point source. The observed spectral energy attenuated within sea ice and in the ice shelf but retains characteristics that can be compared to parametric wave models. Comparison with the Modern‐Era Retrospective Analysis for Research and Applications, Version 2, and ERA5 reanalyses suggests that less than 45% of ocean swell events can be associated with individual Southern Ocean storms, while the majority of the observed wave events cannot be matched with Southern Ocean high wind events. Reanalysis cyclones and winds are often displaced by about 350 km or 10 hr in Modern‐Era Retrospective Analysis for Research and Applications, Version 2, and ERA5 compared to the most likely positions inferred from the seismic spectra. This high fraction of displaced storms in reanalysis products over the South Pacific can be explained by the limited availability of remote sensing observations, primarily caused by the presence of sea ice. Deviation of wave rays from their great circle path by wave‐current interaction plays a minor role.
Real-time transition dynamics and stability of chip-scale dispersion-managed frequency microcombs
Authors: Li Yongnan , Huang Shu-Wei , Li Bowen , Liu Hao , Yang Jinghui , Vinod Abhinav Kumar , Wang Ke , Yu Mingbin , Kwong Dim-Lee , Wang Hui-Tian , Wong Kenneth Kin-Yip , Wong Chee Wei
Published: April 2020

Femtosecond mode-locked laser frequency combs have served as the cornerstone in precision spectroscopy, all-optical atomic clocks, and measurements of ultrafast dynamics. Recently frequency microcombs based on nonlinear microresonators have been examined, exhibiting remarkable precision approaching that of laser frequency combs, on a solid-state chip-scale platform and from a fundamentally different physical origin. Despite recent successes, to date, the real-time dynamical origins and high-power stabilities of such frequency microcombs have not been fully addressed. Here, we unravel the transitional dynamics of frequency microcombs from chaotic background routes to femtosecond mode-locking in real time, enabled by our ultrafast temporal magnifier metrology and improved stability of dispersion-managed dissipative solitons. Through our dispersion-managed oscillator, we further report a stability zone that is more than an order-of-magnitude larger than its prior static homogeneous counterparts, providing a novel platform for understanding ultrafast dissipative dynamics and offering a new path towards high-power frequency microcombs.
A novel silicone derivative of natural osalmid (DCZ0858) induces apoptosis and cell cycle arrest in diffuse large B-cell lymphoma via the JAK2/STAT3 pathway
Authors: Lu Kang , Li Bo , Zhang Hui , Xu Zhijian , Song Dongliang , Gao Lu , Sun Haiguo , Li Liping , Wang Yingcong , Feng Qilin , Chen Gege , Hu Liangning , Wei Rong , Xie Yongsheng , Yu Dandan , Wu Xiaosong , Zhu Weiliang , Shi Jumei
Published: April 2020

Diffuse large B-cell lymphoma (DLBCL) is a highly heterogeneous malignant tumor characterized by diffuse growth. DCZ0858 is a novel small molecule with excellent antitumor effects in DLBCL. This study explored in depth the inhibitory effect of DCZ0858 on DLBCL cell lines. Cell Counting Kit-8 (CCK-8) and plate colony formation assays were used to evaluate cell proliferation levels. Flow cytometry was employed to analyze apoptosis and the cell cycle, and western blotting was used to quantify the expression of cell cycle regulators. The results indicated that DCZ0858 inhibited cell growth in a concentration-dependent and time-dependent manner while inducing no significant toxicity in normal cells. Moreover, DCZ0858 initiated cell apoptosis via both internal and external apoptotic pathways. DCZ0858 also induced cell cycle arrest in the G0/G1 phase, thereby controlling cell proliferation. Further investigation of the molecular mechanism showed that the JAK2/STAT3 pathway was involved in the DCZ0858-mediated antitumor effects and that JAK2 was the key target for DCZ0858 treatment. Knockdown of JAK2 partly weakened the DCZ0858-mediated antitumor effect in DLBCL cells, while JAK2 overexpression strengthened the effect of DCZ0858 in DLBCL cells. Moreover, a similar antitumor effect was observed for DCZ0858 and the JAK2 inhibitor ruxolitinib, and combining the two could significantly enhance cancer-suppressive signaling. Tumor xenograft models showed that DCZ0858 inhibited tumor growth in vivo and had low toxicity in important organs, findings that were consistent with the in vitro data. In summary, DCZ0858 is a promising drug for the treatment of DLBCL.
Associations between calcium and magnesium intake and the risk of incident oesophageal cancer: an analysis of the NIH-AARP Diet and Health Study prospective cohort
Authors: Shah Shailja C. , Dai Qi , Zhu Xiangzhu , Peek Richard M. , Roumie Christianne , Shrubsole Martha J.
Published: April 2020

Background

Risk reduction through dietary modifications is an adjunct strategy for prevention of oesophageal cancer, a leading cause of cancer-related mortality and morbidity worldwide. We aimed to estimate the association between calcium and magnesium intakes and incident oesophageal cancer (OC).

Methods

We conducted a retrospective analysis of the NIH-AARP Diet and Health Study prospective cohort. We used multivariable Cox proportional hazard modeling to estimate the association between total intakes and incident OC overall and by histology (oesophageal squamous cell carcinoma (OSCC) and adenocarcinoma (OAC)). Sensitivity and stratified analyses were performed.

Results

Among 536,359 included respondents, 1414 incident OCs occurred over 6.5 million person-years follow-up time. Increasing dietary calcium intake was associated with an adjusted 32–41% lower risk of OSCC compared to the lowest quartile (p-trend 0.01). There was a positive association between increasing magnesium intake and OAC risk, but only among participants with low calcium:magnesium intake ratios (p-trend 0.04). There was a significant interaction with smoking status.

Conclusions

Based on a retrospective analysis of the NIH-AARP Diet and Health Study prospective cohort, dietary intakes of calcium and magnesium were significantly associated with risk of OSCC and, among certain participants, OAC, respectively. If validated, these findings could inform dietary modifications among at-risk individuals. Mechanistic investigations would provide additional insight.
Reversal of glucocorticoid resistance in paediatric acute lymphoblastic leukaemia is dependent on restoring BIM expression
Authors: Toscan Cara E. , Jing Duohui , Mayoh Chelsea , Lock Richard B.
Published: April 2020

Background

Acute lymphoblastic leukaemia (ALL) is the most common paediatric malignancy. Glucocorticoids form a critical component of chemotherapy regimens and resistance to glucocorticoid therapy is predictive of poor outcome. We have previously shown that glucocorticoid resistance is associated with upregulation of the oncogene C-MYC and failure to induce the proapoptotic gene BIM.

Methods

A high-throughput screening (HTS) campaign was carried out to identify glucocorticoid sensitisers against an ALL xenograft derived from a glucocorticoid-resistant paediatric patient. Gene expression analysis was carried out using Illumina microarrays. Efficacy, messenger RNA and protein analysis were carried out by Resazurin assay, reverse transcription-PCR and immunoblotting, respectively.

Results

A novel glucocorticoid sensitiser, 2-((4,5-dihydro-1H-imidazol-2-yl)thio)-N-isopropyl-N-phenylacetamide (GCS-3), was identified from the HTS campaign. The sensitising effect was specific to glucocorticoids and synergy was observed in a range of dexamethasone-resistant and dexamethasone-sensitive xenografts representative of B-ALL, T-ALL and Philadelphia chromosome-positive ALL. GCS-3 in combination with dexamethasone downregulated C-MYC and significantly upregulated BIM expression in a glucocorticoid-resistant ALL xenograft. The GCS-3/dexamethasone combination significantly increased binding of the glucocorticoid receptor to a novel BIM enhancer, which is associated with glucocorticoid sensitivity.

Conclusions

This study describes the potential of the novel glucocorticoid sensitiser, GCS-3, as a biological tool to interrogate glucocorticoid action and resistance.
Exosome-transmitted lncRNA UFC1 promotes non-small-cell lung cancer progression by EZH2-mediated epigenetic silencing of PTEN expression
Authors: Zang Xueyan , Gu Jianmei , Zhang Jiayin , Shi Hui , Hou Sinan , Xu Xueying , Chen Yanke , Zhang Yu , Mao Fei , Qian Hui , Zhu Taofeng , Xu Wenrong , Zhang Xu
Published: April 2020

Long non-coding RNAs (LncRNAs) have been suggested as important regulators of cancer development and progression in non-small cell lung cancer (NSCLC). Nevertheless, the biological roles and clinical significance of lncRNA UFC1 in NSCLC remain unclear. We detected the expression of UFC1 in tumor tissues, serum, and serum exosomes of NSCLC patients by qRT-PCR. Gene overexpression or silencing were used to examine the biological roles of UFC1 in NSCLC. RNA immunoprecipitation and ChIP assays were performed to evaluate the interaction between UFC1 and enhancer of zeste homolog 2 (EZH2) and the binding of EZH2 to PTEN gene promoter. Rescue study was used to access the importance of PTEN regulation by UFC1 in NSCLC progression. UFC1 expression was upregulated in tumor tissues, serum, and serum exosomes of NSCLC patients and high level of UFC1 was associated with tumor infiltration. UFC1 knockdown inhibited NSCLC cell proliferation, migration and invasion while promoted cell cycle arrest and apoptosis. UFC1 overexpression led to the opposite effects. Mechanistically, UFC1 bound to EZH2 and mediated its accumulation at the promoter region of PTEN gene, resulting in the trimethylation of H3K27 and the inhibition of PTEN expression. UFC1 knockdown inhibited NSCLC growth in mouse xenograft tumor models while the simultaneous depletion of PTEN reversed this effect. NSCLC cells derived exosomes could promote NSCLC cell proliferation, migration and invasion through the transfer of UFC1. Moreover, Exosome-transmitted UFC1 promotes NSCLC progression by inhibiting PTEN expression via EZH2-mediated epigenetic silencing. Exosome-mediated transmit of UFC1 may represent a new mechanism for NSCLC progression and provide a potential marker for NSCLC diagnosis.
LncRNA GAS5 promotes apoptosis as a competing endogenous RNA for miR-21 via thrombospondin 1 in ischemic AKI
Authors: Geng Xuemei , Song Nana , Zhao Shuan , Xu Jiarui , Liu Yong , Fang Yi , Liang Mingyu , Xu Xialian , Ding Xiaoqiang
Published: April 2020

Mounting evidence has indicated that long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) played important roles in renal ischemia/reperfusion (I/R) injury. However, the involvement of lncRNA growth arrest specific 5 (GAS5) in acute kidney injury (AKI) remained largely unexplored. This study aimed to determine possible mechanisms of GAS5 in the renal I/R process. We found that GAS5, noticeably upregulated by renal I/R injury, was further suppressed by delayed IPC while knockdown of miR-21 in vivo before IPC could significantly increased the GAS5 levels. Concurrently, TSP-1 was negatively regulated by miR-21 in vivo and vitro. Additionally, Reciprocal repression of GAS5 and miR-21 was identified. Knockdown of miR-21 in H6R0.5 treated HK-2 cells promoted apoptosis. Co-transfection of miR-21 mimic and pcDNA-GAS5 or pcDNA-Vector were performed, results of which showed that inhibition of miR-21 on TSP-1 could be rescued by overexpression of GAS5. This study suggested that GAS5 facilitated apoptosis by competitively sponging miR-21, which negatively regulated TSP-1 in renal I/R injury. This novel regulatory axis could act as a therapeutic target for AKI in the future.
Phasor field diffraction based reconstruction for fast non-line-of-sight imaging systems
Authors: Liu Xiaochun , Bauer Sebastian , Velten Andreas
Published: April 2020

Non-line-of-sight (NLOS) imaging recovers objects using diffusely reflected indirect light using transient illumination devices in combination with a computational inverse method. While capture systems capable of collecting light from the entire NLOS relay surface can be much more light efficient than single pixel point scanning detection, current reconstruction algorithms for such systems have computational and memory requirements that prevent real-time NLOS imaging. Existing real-time demonstrations also use retroreflective targets and reconstruct at resolutions far below the hardware limits. Our method presented here enables the reconstruction of room-sized scenes from non-confocal, parallel multi-pixel measurements in seconds with less memory usage. We anticipate that our method will enable real-time NLOS imaging when used with emerging single-photon avalanche diode array detectors with resolution only limited by the temporal resolution of the sensor.
Analog content-addressable memories with memristors
Authors: Li Can , Graves Catherine E. , Sheng Xia , Miller Darrin , Foltin Martin , Pedretti Giacomo , Strachan John Paul
Published: April 2020

A content-addressable memory compares an input search word against all rows of stored words in an array in a highly parallel manner. While supplying a very powerful functionality for many applications in pattern matching and search, it suffers from large area, cost and power consumption, limiting its use. Past improvements have been realized by using memristors to replace the static random-access memory cell in conventional designs, but employ similar schemes based only on binary or ternary states for storage and search. We propose a new analog content-addressable memory concept and circuit to overcome these limitations by utilizing the analog conductance tunability of memristors. Our analog content-addressable memory stores data within the programmable conductance and can take as input either analog or digital search values. Experimental demonstrations, scaled simulations and analysis show that our analog content-addressable memory can reduce area and power consumption, which enables the acceleration of existing applications, but also new computing application areas.
β-Catenin and FGFR2 regulate postnatal rosette-based adrenocortical morphogenesis
Authors: Leng Sining , Pignatti Emanuele , Khetani Radhika S. , Shah Manasvi S. , Xu Simiao , Miao Ji , Taketo Makoto M. , Beuschlein Felix , Barrett Paula Q. , Carlone Diana L. , Breault David T.
Published: April 2020

Rosettes are widely used in epithelial morphogenesis during embryonic development and organogenesis. However, their role in postnatal development and adult tissue maintenance remains largely unknown. Here, we show zona glomerulosa cells in the adult adrenal cortex organize into rosettes through adherens junction-mediated constriction, and that rosette formation underlies the maturation of adrenal glomerular structure postnatally. Using genetic mouse models, we show loss of β-catenin results in disrupted adherens junctions, reduced rosette number, and dysmorphic glomeruli, whereas β-catenin stabilization leads to increased adherens junction abundance, more rosettes, and glomerular expansion. Furthermore, we uncover numerous known regulators of epithelial morphogenesis enriched in β-catenin-stabilized adrenals. Among these genes, we show Fgfr2 is required for adrenal rosette formation by regulating adherens junction abundance and aggregation. Together, our data provide an example of rosette-mediated postnatal tissue morphogenesis and a framework for studying the role of rosettes in adult zona glomerulosa tissue maintenance and function.
The circadian clock and darkness control natural competence in cyanobacteria
Authors: Taton Arnaud , Erikson Christian , Yang Yiling , Rubin Benjamin E. , Rifkin Scott A. , Golden James W. , Golden Susan S.
Published: April 2020

The cyanobacterium Synechococcus elongatus is a model organism for the study of circadian rhythms. It is naturally competent for transformation—that is, it takes up DNA from the environment, but the underlying mechanisms are unclear. Here, we use a genome-wide screen to identify genes required for natural transformation in S. elongatus, including genes encoding a conserved Type IV pilus, genes known to be associated with competence in other bacteria, and others. Pilus biogenesis occurs daily in the morning, while natural transformation is maximal when the onset of darkness coincides with the dusk circadian peak. Thus, the competence state in cyanobacteria is regulated by the circadian clock and can adapt to seasonal changes of day length.
Transient microglial absence assists postmigratory cortical neurons in proper differentiation
Authors: Hattori Yuki , Naito Yu , Tsugawa Yoji , Nonaka Shigenori , Wake Hiroaki , Nagasawa Takashi , Kawaguchi Ayano , Miyata Takaki
Published: April 2020

In the developing cortex, postmigratory neurons accumulate in the cortical plate (CP) to properly differentiate consolidating subtype identities. Microglia, despite their extensive surveying activity, temporarily disappear from the midembryonic CP. However, the mechanism and significance of this absence are unknown. Here, we show that microglia bidirectionally migrate via attraction by CXCL12 released from the meninges and subventricular zone and thereby exit the midembryonic CP. Upon nonphysiological excessive exposure to microglia in vivo or in vitro, young postmigratory and in vitro-grown CP neurons showed abnormal differentiation with disturbed expression of the subtype-associated transcription factors and genes implicated in functional neuronal maturation. Notably, this effect is primarily attributed to interleukin 6 and type I interferon secreted by microglia. These results suggest that “sanctuarization” from microglia in the midembryonic CP is required for neurons to appropriately fine-tune the expression of molecules needed for proper differentiation, thus securing the establishment of functional cortical circuit.
Genome-wide association study identifies 143 loci associated with 25 hydroxyvitamin D concentration
Authors: Revez Joana A. , Lin Tian , Qiao Zhen , Xue Angli , Holtz Yan , Zhu Zhihong , Zeng Jian , Wang Huanwei , Sidorenko Julia , Kemper Kathryn E. , Vinkhuyzen Anna A. E. , Frater Julanne , Eyles Darryl , Burne Thomas H. J. , Mitchell Brittany , Martin Nicholas G. , Zhu Gu , Visscher Peter M. , Yang Jian , Wray Naomi R. , McGrath John J.
Published: April 2020

Vitamin D deficiency is a candidate risk factor for a range of adverse health outcomes. In a genome-wide association study of 25 hydroxyvitamin D (25OHD) concentration in 417,580 Europeans we identify 143 independent loci in 112 1-Mb regions, providing insights into the physiology of vitamin D and implicating genes involved in lipid and lipoprotein metabolism, dermal tissue properties, and the sulphonation and glucuronidation of 25OHD. Mendelian randomization models find no robust evidence that 25OHD concentration has causal effects on candidate phenotypes (e.g. BMI, psychiatric disorders), but many phenotypes have (direct or indirect) causal effects on 25OHD concentration, clarifying the epidemiological relationship between 25OHD status and the health outcomes examined in this study.
Quantum capacitance mediated carbon nanotube optomechanics
Authors: Blien Stefan , Steger Patrick , Hüttner Niklas , Graaf Richard , Hüttel Andreas K.
Published: April 2020

Cavity optomechanics allows the characterization of a vibration mode, its cooling and quantum manipulation using electromagnetic fields. Regarding nanomechanical as well as electronic properties, single wall carbon nanotubes are a prototypical experimental system. At cryogenic temperatures, as high quality factor vibrational resonators, they display strong interaction between motion and single-electron tunneling. Here, we demonstrate large optomechanical coupling of a suspended carbon nanotube quantum dot and a microwave cavity, amplified by several orders of magnitude via the nonlinearity of Coulomb blockade. From an optomechanically induced transparency (OMIT) experiment, we obtain a single photon coupling of up to g0 = 2π ⋅ 95 Hz. This indicates that normal mode splitting and full optomechanical control of the carbon nanotube vibration in the quantum limit is reachable in the near future. Mechanical manipulation and characterization via the microwave field can be complemented by the manifold physics of quantum-confined single electron devices.
Plasmonic ommatidia for lensless compound-eye vision
Authors: Kogos Leonard C. , Li Yunzhe , Liu Jianing , Li Yuyu , Tian Lei , Paiella Roberto
Published: April 2020

The vision system of arthropods such as insects and crustaceans is based on the compound-eye architecture, consisting of a dense array of individual imaging elements (ommatidia) pointing along different directions. This arrangement is particularly attractive for imaging applications requiring extreme size miniaturization, wide-angle fields of view, and high sensitivity to motion. However, the implementation of cameras directly mimicking the eyes of common arthropods is complicated by their curved geometry. Here, we describe a lensless planar architecture, where each pixel of a standard image-sensor array is coated with an ensemble of metallic plasmonic nanostructures that only transmits light incident along a small geometrically-tunable distribution of angles. A set of near-infrared devices providing directional photodetection peaked at different angles is designed, fabricated, and tested. Computational imaging techniques are then employed to demonstrate the ability of these devices to reconstruct high-quality images of relatively complex objects.
Exome sequencing of familial high-grade serous ovarian carcinoma reveals heterogeneity for rare candidate susceptibility genes
Authors: Subramanian Deepak N. , Zethoven Magnus , McInerny Simone , Morgan James A. , Rowley Simone M. , Lee Jue Er Amanda , Li Na , Gorringe Kylie L. , James Paul A. , Campbell Ian G.
Published: April 2020

High-grade serous ovarian carcinoma (HGSOC) has a significant hereditary component, approximately half of which cannot be explained by known genes. To discover genes, we analyse germline exome sequencing data from 516BRCA1/2-negative women with HGSOC, focusing on genes enriched with rare, protein-coding loss-of-function (LoF) variants. Overall, there is a significant enrichment of rare protein-coding LoF variants in the cases (p < 0.0001, chi-squared test). Only thirty-four (6.6%) have a pathogenic variant in a known or proposed predisposition gene. Few genes have LoF mutations in more than four individuals and the majority are detected in one individual only. Forty-three highly-ranked genes are identified with three or more LoF variants that are enriched by three-fold or more compared to GnomAD. These genes represent diverse functional pathways with relatively few involved in DNA repair, suggesting that much of the remaining heritability is explained by previously under-explored genes and pathways.
Artificially decreasing cortical tension generates aneuploidy in mouse oocytes
Authors: Bennabi Isma , Crozet Flora , Nikalayevich Elvira , Chaigne Agathe , Letort Gaëlle , Manil-Ségalen Marion , Campillo Clément , Cadart Clotilde , Othmani Alice , Attia Rafaele , Genovesio Auguste , Verlhac Marie-Hélène , Terret Marie-Emilie
Published: April 2020

Human and mouse oocytes’ developmental potential can be predicted by their mechanical properties. Their development into blastocysts requires a specific stiffness window. In this study, we combine live-cell and computational imaging, laser ablation, and biophysical measurements to investigate how deregulation of cortex tension in the oocyte contributes to early developmental failure. We focus on extra-soft cells, the most common defect in a natural population. Using two independent tools to artificially decrease cortical tension, we show that chromosome alignment is impaired in extra-soft mouse oocytes, despite normal spindle morphogenesis and dynamics, inducing aneuploidy. The main cause is a cytoplasmic increase in myosin-II activity that could sterically hinder chromosome capture. We describe here an original mode of generation of aneuploidies that could be very common in oocytes and could contribute to the high aneuploidy rate observed during female meiosis, a leading cause of infertility and congenital disorders.
Lamella-nanostructured eutectic zinc–aluminum alloys as reversible and dendrite-free anodes for aqueous rechargeable batteries
Authors: Wang Sheng-Bo , Ran Qing , Yao Rui-Qi , Shi Hang , Wen Zi , Zhao Ming , Lang Xing-You , Jiang Qing
Published: April 2020

Metallic zinc is an attractive anode material for aqueous rechargeable batteries because of its high theoretical capacity and low cost. However, state-of-the-art zinc anodes suffer from low coulombic efficiency and severe dendrite growth during stripping/plating processes, hampering their practical applications. Here we show that eutectic-composition alloying of zinc and aluminum as an effective strategy substantially tackles these irreversibility issues by making use of their lamellar structure, composed of alternating zinc and aluminum nanolamellas. The lamellar nanostructure not only promotes zinc stripping from precursor eutectic Zn88Al12 (at%) alloys, but produces core/shell aluminum/aluminum sesquioxide interlamellar nanopatterns in situ to in turn guide subsequent growth of zinc, enabling dendrite-free zinc stripping/plating for more than 2000 h in oxygen-absent aqueous electrolyte. These outstanding electrochemical properties enlist zinc-ion batteries constructed with Zn88Al12 alloy anode and KxMnO2 cathode to deliver high-density energy at high levels of electrical power and retain 100% capacity after 200 hours.
Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum
Authors: Sogues Adrià , Martinez Mariano , Gaday Quentin , Ben Assaya Mathilde , Graña Martin , Voegele Alexis , VanNieuwenhze Michael , England Patrick , Haouz Ahmed , Chenal Alexandre , Trépout Sylvain , Duran Rosario , Wehenkel Anne Marie , Alzari Pedro M.
Published: April 2020

The mechanisms of Z-ring assembly and regulation in bacteria are poorly understood, particularly in non-model organisms. Actinobacteria, a large bacterial phylum that includes the pathogen Mycobacterium tuberculosis, lack the canonical FtsZ-membrane anchors and Z-ring regulators described for E. coli. Here we investigate the physiological function of Corynebacterium glutamicum SepF, the only cell division-associated protein from Actinobacteria known to interact with the conserved C-terminal tail of FtsZ. We show an essential interdependence of FtsZ and SepF for formation of a functional Z-ring in C. glutamicum. The crystal structure of the SepF–FtsZ complex reveals a hydrophobic FtsZ-binding pocket, which defines the SepF homodimer as the functional unit, and suggests a reversible oligomerization interface. FtsZ filaments and lipid membranes have opposing effects on SepF polymerization, indicating that SepF has multiple roles at the cell division site, involving FtsZ bundling, Z-ring tethering and membrane reshaping activities that are needed for proper Z-ring assembly and function.
Site-selective doping of ordered charge states in magnetite
Authors: Pachoud E. , Cumby J. , Perversi G. , Wright J. P. , Attfield J. P.
Published: April 2020

Charge ordering creates a spontaneous array of differently charged ions and is associated with electronic phenomena such as superconductivity, colossal magnetoresistances (CMR), and multiferroicity. Charge orders are usually suppressed by chemical doping and site selective doping of a charge ordered array has not previously been demonstrated. Here we show that selective oxidation of one out of eight distinct Fe2+ sites occurs within the complex Fe2+/Fe3+ ordered structure of 2%-doped magnetite (Fe3O4), while the rest of the charge and orbitally ordered network remains intact. This ‘charge order within a charge order’ is attributed to the relative instability of the trimeron distortion surrounding the selected site. Our discovery suggests that similar complex charge ordered arrays could be used to provide surface sites for selective redox reactions, or for storing information by doping specific sites.
East Siberian Arctic inland waters emit mostly contemporary carbon
Authors: Dean Joshua F. , Meisel Ove H. , Martyn Rosco Melanie , Marchesini Luca Belelli , Garnett Mark H. , Lenderink Henk , van Logtestijn Richard , Borges Alberto V. , Bouillon Steven , Lambert Thibault , Röckmann Thomas , Maximov Trofim , Petrov Roman , Karsanaev Sergei , Aerts Rien , van Huissteden Jacobus , Vonk Jorien E. , Dolman A. Johannes
Published: April 2020

Inland waters (rivers, lakes and ponds) are important conduits for the emission of terrestrial carbon in Arctic permafrost landscapes. These emissions are driven by turnover of contemporary terrestrial carbon and additional pre-aged (Holocene and late-Pleistocene) carbon released from thawing permafrost soils, but the magnitude of these source contributions to total inland water carbon fluxes remains unknown. Here we present unique simultaneous radiocarbon age measurements of inland water CO2, CH4and dissolved and particulate organic carbon in northeast Siberia during summer. We show that >80% of total inland water carbon was contemporary in age, but pre-aged carbon contributed >50% at sites strongly affected by permafrost thaw. CO2and CH4were younger than dissolved and particulate organic carbon, suggesting emissions were primarily fuelled by contemporary carbon decomposition. Our findings reveal that inland water carbon emissions from permafrost landscapes may be more sensitive to changes in contemporary carbon turnover than the release of pre-aged carbon from thawing permafrost.
Nonreciprocal directional dichroism of a chiral magnet in the visible range
Authors: Yokosuk Michael O. , Kim Heung-Sik , Hughey Kendall D. , Kim Jaewook , Stier Andreas V. , O’Neal Kenneth R. , Yang Junjie , Crooker Scott A. , Haule Kristjan , Cheong Sang-Wook , Vanderbilt David , Musfeldt Janice L.
Published: April 2020

Nonreciprocal directional dichroism is an unusual light–matter interaction that gives rise to diode-like behavior in low-symmetry materials. The chiral varieties are particularly scarce due to the requirements for strong spin–orbit coupling, broken time-reversal symmetry, and a chiral axis. Here we bring together magneto-optical spectroscopy and first-principles calculations to reveal high-energy, broadband nonreciprocal directional dichroism in Ni3TeO6 with special focus on behavior in the metamagnetic phase above 52 T. In addition to demonstrating this effect in the magnetochiral configuration, we explore the transverse magnetochiral orientation in which applied field and light propagation are orthogonal to the chiral axis and, by so doing, uncover an additional configuration with a unique nonreciprocal response in the visible part of the spectrum. In a significant conceptual advance, we use first-principles methods to analyze how the Ni2+d-to-d on-site excitations develop magneto-electric character and present a microscopic model that unlocks the door to theory-driven discovery of chiral magnets with nonreciprocal properties.
A synthetic energy dataset for non-intrusive load monitoring in households
Authors: Klemenjak Christoph , Kovatsch Christoph , Herold Manuel , Elmenreich Wilfried
Published: April 2020

Research on smart grid technologies is expected to result in effective climate change mitigation. Non-Intrusive Load Monitoring (NILM) is seen as a key technique for enabling innovative smart-grid services. By breaking down the energy consumption of households and industrial facilities into its components, NILM techniques provide information on present appliances and can be applied to perform diagnostics. As with related Machine Learning problems, research and development requires a sufficient amount of data to train and validate new approaches. As a viable alternative to collecting datasets in buildings during expensive and time-consuming measurement campaigns, the idea of generating synthetic datasets for NILM gain momentum recently. With SynD, we present a synthetic energy dataset with focus on residential buildings. We release 180 days of synthetic power data on aggregate level (i.e. mains) and individual appliances. SynD is the result of a custom simulation process that relies on power traces of real household appliances. In addition, we present several case studies that demonstrate similarity of our dataset and four real-world energy datasets.
Amplicon sequencing analysis of arbuscular mycorrhizal fungal communities colonizing maize roots in different cover cropping and tillage systems
Authors: Higo Masao , Tatewaki Yuya , Iida Karen , Yokota Kana , Isobe Katsunori
Published: April 2020

Our understanding regarding the influence of intensive agricultural practices, including cover cropping and tillage, on communities of arbuscular mycorrhizal fungi (AMF) is lacking. This would prove to be an obstacle in the improvement of current maize (Zea mays L.) production. Therefore, using amplicon sequencing, we aimed to clarify how AMF communities and their diversity in maize roots vary under different cover cropping systems and two types of tillage (rotary and no tillage). Two kinds of cover crops (hairy vetch and brown mustard) and fallow treatments were established with rotary or no tillage in rotation with maize crops. Tillage and no tillage yielded a set of relatively common AMF operational taxonomic units (OTUs) in the maize crops, representing 78.3% of the total OTUs. The percentage of maize crop OTUs that were specific to only tillage and no tillage were 9.6% and 12.0%, respectively. We found that tillage system significantly altered the AMF communities in maize roots. However, the AMF communities of maize crops among cover cropping treatments did not vary considerably. Our findings indicate that compared with cover cropping, tillage may shape AMF communities in maize more strongly.
Publisher Correction: Collective behavior of oscillating electric dipoles
Authors: Olmi Simona , Gori Matteo , Donato Irene , Pettini Marco
Published: April 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Anemia and its associated factors among Adolescents in Kuwait
Authors: Shaban Lemia , Al-Taiar Abdullah , Rahman Abdur , Al-Sabah Reem , Mojiminiyi Olusegun
Published: April 2020

We estimated the prevalence of anemia among school children and investigated factors associated with this problem in Kuwait. A cross-sectional study was conducted on 1415 adolescents randomly selected from middle schools in Kuwait. Hemoglobin, iron, ferritin, folate and vitamin B12, in addition to many other laboratory indicators, were measured in a venous blood sample. Data on risk factors for anemia were collected from parents and adolescents. Multiple logistic regression was used to investigate factors associated with anemia. The prevalence of anemia was 8.06% (95% CI: 6.69–9.60%), which was significantly higher among females compared to males (10.96% vs. 5.04%; p < 0.001). Mean (SD) Hb level was 133.7 (9.89) g/L and 130.00 (10.48) g/L among males and females, respectively (p < 0.001). The prevalence of mild, moderate and severe anemia was 5.94%, 1.91% and 0.21%, respectively. Gender, age, iron concentration and ferritin were associated with anemia in multivariable analysis. These data indicate that anemia among school children in Kuwait is of mild public health significance. Further reduction in anemia in school girls should focus on correcting iron deficiency. Surveillance systems for anemia may consider using a cut-off point that is specific for the method of blood sampling and the method of Hb measurement.