bluuebunny/crossref_metadata_2025 huggingface dataset:
The inevitability of arbuscular mycorrhiza for sustainability in organic agriculture—A critical review
Authors: George Nirmal Philip , Ray Joseph George
Published: February 2023

The arbuscular mycorrhizal fungi (AMF) are significant fertility-promoting microbes in soils. They enable soil fertility, soil-health and boost crop productivity. There are generalist and specialist groups among AMF in natural soils. Optimized use of specific AMF concerning crops and soils can improve agricultural sustainability. Thus, AMF is becoming an inevitable biological tool for improving crop productivity and soil health. Especially in the context of chemicalized agriculture undermining the sustainability of food security, safety, and human and ecosystem health, alternative agricultural means have become inevitable. Therefore, AMF has become essential in nature-friendly, organic agriculture. Of such farm fields, natural biological activity is enhanced to sustain soil fertility. Crops show increased innate immunity against pests and diseases in many such systems. Moreover, ecosystems remain healthy, and the soil is teeming with life in such farms. The primary goal of the review was a thorough critical analysis of the literature on AMF in organic agriculture to assess its efficiency as an ecotechnological tool in sustainable agricultural productivity. The novelty is that this is the first comprehensive review of literature on AMF concerning all aspects of organic agriculture. A vital systematic approach to the exhaustive literature collected using regular databases on the theme is followed for synthesizing the review. The review revealed the essentiality of utilizing specific mycorrhizal species, individually or in consortia, in diverse environmental settings to ensure sustainable organic crop production. However, for the exact usage of specific AMF in sustainable organic agriculture, extensive exploration of them in traditional pockets of specific crop cultivations of both chemical and organic fields and wild environments is required. Moreover, intensive experimentations are also necessary to assess them individually, in combinations, and associated with diverse beneficial soil bacteria.
Florid cystitis glandularis (intestinal type) with mucus extravasation: Two case reports and literature review
Authors: Zhang Tao , Yin Si-Fan , Feng Wen-Bo , Ke Chang-Xing
Published: February 2023

Background

Cystitis glandularis is a common bladder epithelial lesion characterized by hyperplasia and metaplasia of the bladder mucosa epithelium. The pathogenesis of cystitis glandularis of the intestinal type is unknown and less common. When cystitis glandularis (intestinal type) is extremely severely differentiated, it is called florid cystitis glandularis (the occurrence is extremely rare).

Case summary

Both patients were middle-aged men. In patient 1, the lesion was also seen in the posterior wall and was diagnosed more than 1 year ago as cystitis glandularis with urethral stricture. Patient 2 was examined for symptoms such as hematuria and was found to have an occupied bladder; both were treated surgically, and the postoperative pathology was diagnosed as florid cystitis glandularis (intestinal type), with mucus extravasation.

Conclusion

The pathogenesis of cystitis glandularis (intestinal type) is unknown and less common. When cystitis glandularis of the intestinal type is extremely severely differentiated, we call it florid cystitis glandularis. It is more common in the bladder neck and trigone. The clinical manifestations are mainly symptoms of bladder irritation, or hematuria as the main complaint, which rarely leads to hydronephrosis. Imaging is nonspecific and the diagnosis depends on pathology. Surgical excision of the lesion is possible. Due to the malignant potential of cystitis glandularis of intestinal type, postoperative follow-up is required.
Obtaining an animal welfare status in Norwegian dairy herds—A mountain to climb
Authors: Barry Conor , Ellingsen-Dalskau Kristian , Garmo Randi Therese , Grønmo Kischel Stine , Winckler Christoph , Kielland Camilla
Published: February 2023

Introduction

Knowing the national status of animal welfare, one can identify welfare problems and set a benchmark against which improvements can be compared. Such a status is potentially invaluable for tangible, sustained animal welfare improvement. The objective of this cross-sectional study was to report the status of animal welfare in Norwegian loose-housed dairy herds as assessed using the Welfare Quality®Assessment Protocol. Additionally, we investigated if the welfare status varied on a regional basis.

Methods

In total, 155 herds in eight of Norway's eleven counties were assessed by six trained Welfare Quality®assessors. This article presents the herd prevalences of common welfare issues in dairy production in Norway, as well as integrated welfare scores. To determine whether welfare status varied regionally in Norway, generalized linear modeling was used to estimate the mean welfare score for five regions in the four Welfare Quality®principles: A. Good feeding, B. Good housing, C. Good health, and D. Appropriate behavior. These estimated mean welfare scores and their 95% confidence intervals were subsequently assessed for significant variation.

Results

Encouraging findings included the low mean herd prevalence of ‘very lean' cows (3.0%) and the high proportion of cows (59.8%) which could be touched during avoidance distance testing, indicating a positive relationship between stockpeople and their cattle. Challenges affecting the welfare of Norwegian dairy cows were also identified. Of particular concern were issues related to the cows' environment such as prolonged times needed to complete lying down movements and integument alterations. No herd was completely free of changes to the integument and, on average, 77.9% of each herd were affected either mildly or severely. Animal welfare did not appear to vary much between the five regions assessed. Our investigation revealed significant regional variation between two regions (Trøndelag and Vestlandet North) in only the Welfare Quality®principle Good housing (p< 0.01).

Discussion

The almost complete absence of regional variation demonstrates that animal welfare status generally varies most at herd level. In conclusion, both welfare challenges and encouraging findings were identified in loose-housed Norwegian dairy herds. To improve animal welfare, herd-specific interventions are most likely to be effective in these herds.
COPA3 peptide supplementation alleviates the heat stress of chicken fibroblasts
Authors: Siddiqui Sharif Hasan , Khan Mousumee , Park Jinryong , Lee Jeongeun , Choe Hosung , Shim Kwanseob , Kang Darae
Published: February 2023

Heat stress inhibits cellular proliferation and differentiation through the production of reactive oxygen species. Under stress conditions, antioxidant drugs promote stable cellular function by reducing the stress level. We sought to demonstrate 9-mer disulfide dimer peptide (COPA3) supplementation stabilizes fibroblast proliferation and differentiation even under heat stress conditions. In our study, fibroblasts were assigned to two different groups based on the temperature, like 38°C group presented as Control - and 43°C group presented as Heat Stress-. Each group was subdivided into two groups depending upon COPA3 treatment, like 38°C + COPA3 group symbolized Control+ and the 43°C + COPA3 group symbolized as Heat Stress+. Heat stress was observed to decrease the fibroblast viability and function and resulted in alterations in the fibroblast shape and cytoskeleton structure. In contrast, COPA3 stabilized the fibroblast viability, shape, and function. Moreover, heat stress and COPA3 were found to have opposite actions with respect to energy production, which facilitates the stabilization of cellular functions by increasing the heat tolerance capacity. The gene expression levels of antioxidant and heat shock proteins were higher after heat stress. Additionally, heat stress promotes the mitogen-activated protein kinase/ extracellular signal-regulated kinase–nuclear factor erythroid 2-related factor 2 (MAPK/ERK-Nrf2). COPA3 maintained the MAPK/ERK-Nrf2 gene expressions that promote stable fibroblast proliferation, and differentiation as well as suppress apoptosis. These findings suggest that COPA3 supplementation increases the heat tolerance capacity, viability, and functional activity of fibroblasts.
The Need for Speed: A Fast Guessing Entropy Calculation for Deep Learning-Based SCA
Authors: Perin Guilherme , Wu Lichao , Picek Stjepan
Published: February 2023

The adoption of deep neural networks for profiling side-channel attacks opened new perspectives for leakage detection. Recent publications showed that cryptographic implementations featuring different countermeasures could be broken without feature selection or trace preprocessing. This success comes with a high price: an extensive hyperparameter search to find optimal deep learning models. As deep learning models usually suffer from overfitting due to their high fitting capacity, it is crucial to avoid over-training regimes, which require a correct number of epochs. For that, early stopping is employed as an efficient regularization method that requires a consistent validation metric. Although guessing entropy is a highly informative metric for profiling side-channel attacks, it is time-consuming, especially if computed for all epochs during training, and the number of validation traces is significantly large. This paper shows that guessing entropy can be efficiently computed during training by reducing the number of validation traces without affecting the efficiency of early stopping decisions. Our solution significantly speeds up the process, impacting the performance of the hyperparameter search and overall profiling attack. Our fast guessing entropy calculation is up to 16× faster, resulting in more hyperparameter tuning experiments and allowing security evaluators to find more efficient deep learning models.
Review of Discrete Element Method Simulations of Soil Tillage and Furrow Opening
Authors: Aikins Kojo Atta , Ucgul Mustafa , Barr James B. , Awuah Emmanuel , Antille Diogenes L. , Jensen Troy A. , Desbiolles Jacky M. A.
Published: February 2023

In agricultural machinery design and optimization, the discrete element method (DEM) has played a major role due to its ability to speed up the design and manufacturing process by reducing multiple prototyping, testing, and evaluation under experimental conditions. In the field of soil dynamics, DEM has been mainly applied in the design and optimization of soil-engaging tools, especially tillage tools and furrow openers. This numerical method is able to capture the dynamic and bulk behaviour of soils and soil–tool interactions. This review focused on the various aspects of the application of DEM in the simulation of tillage and furrow opening for tool design optimization. Different contact models, particle sizes and shapes, and calibration techniques for determining input parameters for tillage and furrow opening research have been reviewed. Discrete element method predictions of furrow profiles, disturbed soil surface profiles, soil failure, loosening, disturbance parameters, reaction forces, and the various types of soils modelled with DEM have also been highlighted. This pool of information consolidates existing working approaches used in prior studies and helps to identify knowledge gaps which, if addressed, will advance the current soil dynamics modelling capability.
Abscisic-Acid-Modulated Stomatal Conductance Governs High-Temperature Stress Tolerance in Rice Accessions
Authors: Malini M. K. , Karwa Sourabh , Priyadarsini Payal , Kumar Pramod , Nagar Shivani , Kumar Mahesh , Kumar Sudhir , Chinnusamy Viswanathan , Pandey Renu , Pal Madan
Published: February 2023

Rising air temperature is a major constraint for crop productivity under the current climate change scenario. Rice crops are known to be sensitive to high-temperature (HT) stress at anthesis and post-anthesis stages. Photosynthesis is an important metabolic process and is affected by HT stress. A pot study was planned to screen a set of seventy-three Indian rice accessions based upon changes in the rate of photosynthesis (Pn) and related gas exchange traits under HT, and to characterize the contrasting rice accessions for component traits of HT stress tolerance. All accessions were raised under ambient temperature (AT) until the booting stage and exposed to HT using controlled chambers at anthesis and post-anthesis. HT exposure led to a large reduction (up to 50%) in Pn, but stomatal conductance (gs) and the rate of transpiration (E) increased significantly across the rice accessions. Based on the photosynthetic response under HT, two contrasting rice accessions (IRGC 135883, tolerant, and IRGC 127222, sensitive) were selected and characterized for HT tolerance, along with an NL-44 check. Among them, Pn decreased marginally but gs and E showed significant increases under HT in the tolerant accession, while sensitive accession showed an up to 50% reduction in Pn and marginal increase in gs and E. No significant changes were recorded for chlorophyll fluorescence (Fv/Fm) in both the genotypes, but tissue temperature depression (TTD) was higher in IRGC 135883 accession under HT. Endogenous abscisic acid (ABA) content increased under HT in the flag leaf of both the accessions, and the highest increase was observed in the sensitive accession. Similarly, spikelet fertility and grain yield showed large reductions in sensitive rice accession under HT. A large increase in ABA concentration in the leaves of the sensitive rice accession might be affecting its gs and cooling capacity under an HT environment. Finally, the study concludes that tolerant rice accessions can be recommended as donors and exploited in future rice breeding programs for developing climate-resilient rice genotypes.
Soil Density Characterization in Management Zones Based on Apparent Soil Electrical Conductivity in Two Field Systems: Rainfeed and Center-Pivot Irrigation
Authors: Bottega Eduardo Leonel , Marin Cristielle König , Oliveira Zanandra Boff de , Lamb Christiano de Carvalho , Amado Telmo Jorge Carneiro
Published: February 2023

Understanding the spatial variability of factors that influence crop yield is essential to apply site-specific management. The present study aimed to evaluate apparent soil electrical conductivity (ECa) in two fields (A = rainfeed; B = central-pivot irrigation), based on delimited management zones (MZs). In each MZ, the soil density (Sd) was characterized at two soil depths, and whether the delimitation of MZs, based on the spatial variability of ECa, was able to identify regions of the field with different Sd was assessed. In general, MZs with the highest mean value of ECa also presented the highest mean values of Sd. The highest Sd values were observed in the 0.1–0.2 m layer, regardless of the studied area. Regardless of soil texture, the proposed ECa was able to detect in-field differences in Sd. The delimitation of MZs, based on the spatial variability of ECa mapping, was able to differentiate the mean values of Sd between MZ 1 (1.53 g cm−3) and MZ 2 (1.67 g cm−3) in field A, in the 0.1–0.2 m layer. A statistical difference was observed for the mean values of Sd, in MZ 1, at layer 0.1–0.2 m, when comparing the two fields: A (1.53 g cm−3) and B (1.64 g cm−3). We suggest that further studies should be carried out to confirm the efficiency of ECa in detecting the soil bulk density at different soil depths.
The Inorganic Carbon Fixation Improved by Long-Term Manure Fertilization in Kastanozems under Rotation System of North China
Authors: Tang Lingyun , Ren Fengling , Li Yalin , Duan Yu , Sun Nan , Zhao Peiyi , Li Yuejin , Xu Minggang
Published: February 2023

In China, manure is the most abundant organic fertilizer product. Understanding the changes in soil inorganic carbon (SIC) resulting from long-term manure fertilization is vital for accurately evaluating agricultural soil carbon stocks and predicting global change. However, a comprehensive and quantitative understanding of the impacts of long-term fertilization on SIC is lacking. This study was conducted to assess the effects of SIC changes in topsoil (0–20 cm), aggregates of kastanozems and influencing factors under the potato-rape-naked oats cultivation system after 16 years of long-term different fertilization in Wuchuan station. The results showed that 16 years of application of manure promoted the fixation of SIC by 2.25 t ha−1–3.25 t ha−1. As soil organic carbon (SOC) content, exchangeable calcium and magnesium concentrations in free coarse particulate organic matter (cfPOM) increased, the crystallization of carbonate was promoted at the aggregate level. The distribution proportion of free-coarse particulate organic matter (cfPOM) and microaggregates in mineral-related organic matter (iMOM) were also increased which affected the content of SIC. However, the pH value of NPKM treatment was lower than that of M treatment, which reduced the amount of carbonate crystallization. Thus, the application of manure alone was the best way to promote the fixation of SIC in topsoil rather than manure combined with chemical NPK fertilize. This work provides a new insight into the conversion of inorganic carbon, which is beneficial to promote the sequestration of inorganic carbon.
Application of Synthetic Consortia for Improvement of Soil Fertility, Pollution Remediation, and Agricultural Productivity: A Review
Authors: Chaudhary Parul , Xu Miao , Ahamad Lukman , Chaudhary Anuj , Kumar Govind , Adeleke Bartholomew Saanu , Verma Krishan K. , Hu Dian-Ming , Širić Ivan , Kumar Pankaj , Popescu Simona M. , Abou Fayssal Sami
Published: February 2023

Climate change, inadequate possessions, and land degradation all pose obstacles to modern agriculture. In the current scenario, the agriculture industry is mainly dependent on the use of chemical-based pesticides and fertilizers that impact soil health and crop productivity. Moreover, water scarcity leads farmers in drastically affected regions to use heavy metal-enriched water sources mainly originating from industrial sources for field crops irrigation. Soil pollutants can be carried into the human body via dust and water, creating negative health effects varying from simple symptoms, e.g., nausea and diarrhea and reaching death in critical cases. Thus, to clean soil contaminants, and improve soil fertility and agricultural production, alternatives to chemical fertilizers must be developed. Therefore, using beneficial microbes found in plant-associated soil microorganisms offers an effective strategy to alleviate some of these challenges, improving soil fertility, and crop yield, and protecting plants from stress conditions. Through the use of synergistic interactions, the synthetic consortium strategy seeks to improve the stability of microbial communities. In this review, synthetic consortia and their potential use in agriculture were discussed. Further, engineering new effective synthetic consortia was suggested as an effective approach in the concept of environmental bioremediation of soil pollutants and contaminants.
The TIR-Type NLR Protein Is Involved in the Regulation of Phelipanche aegyptiaca Resistance in Cucumis melo
Authors: Xiao Lifeng , Zhao Qiuyue , Cao Xiaolei , Yao Zhaoqun , Zhao Sifeng
Published: February 2023

Phelipanche aegyptiaca is an obligate holoparasite that causes serious negative effects on crop growth and productivity, effective control of which is difficult due to its unique biological characteristics. In this study, we performed a comparative transcriptome analysis of resistant and susceptible Cucumis melo cultivars (KR1326 and K1237) inoculated with P. aegyptiaca. CmNLR (encodes a TIR-type NLR protein, consistently highly expressed in KR1326 roots) and CmNLRh (homologous gene of CmNLR) were cloned and overexpressed in K1237 roots to verify whether the TIR-type NLR protein can enhance C. melo resistance to P. aegyptiaca. The variations in enzymes related to active oxygen metabolism were further detected in transformed roots. The results showed that (1) some differentially expressed genes (DEGs) were enriched in pathways associated with active oxygen scavenging; (2) several DEGs encoded transcription factors, calcium channel proteins, and receptor-like proteins were upregulated and expressed in KR1326 roots; (3) the complete CmNLR and CmNLRh proteins prevented P. aegyptiaca from connecting to the vascular system of C. melo roots; and (4) stronger active oxygen burst and scavenging capacity were detected in transformed roots. We herein demonstrated that the TIR-type NLR protein confers C. melo resistance to P. aegyptiaca. The results provided clues for finding a new direction for host resistance against parasitic plants and shed new light on the cultivation of resistant varieties to control P. aegyptiaca.
Nematodes Associated with Terrestrial Slugs in Mid-Atlantic (Delaware, USA) Soybean
Authors: Kunkel Brian , Cissel William J. , Tooker John F. , Howe Dana K. , Denver Dee R. , Mc Donnell Rory J. , Hiltpold Ivan
Published: February 2023

Slugs are cryptic terrestrial pests that target a wide range of crops and are especially damaging to seedlings. Management of these invertebrates mostly relies on synthetic chemistry. These molecules can be efficient against slugs and snails but can be toxic to other organisms (e.g., dogs) and harmful to the environment (e.g., leaching into surface and groundwater). The usage of pathogenic nematodes has been effective in several crops and European countries. A survey was conducted to investigate the presence of natural populations of malacopathogenic nematodes in soybean in the mid-Atlantic region. Slugs were sampled in nine fields across Delaware at various distances from the field edges (0 m, 5 m, 10 m, 15 m). Soil cover and soil type were also recorded. Invertebrates were brought back to the laboratory. Slugs were monitored for four weeks, and mortality was classified into one of three categories: (1) death with the presence of nematodes; (2) death with the presence of fungi; (3) death without the presence of nematodes or fungi. Nematodes associated with slugs were identified based on 18S rRNA sequencing. The distance from the field edge did not impact the number of trapped slugs and the incidence of slug death associated with the presence of nematodes. Overall, nematodes were collected from ca. 20% of the slug cadavers, and most have previously been associated with slugs (ca. 35% of deaths associated with fungi and ca. 45% not associated with nematodes or fungi). The number of captured slugs and slug death associated with the presence of nematodes were positively correlated with ground cover. Soil type impacted both the number of captured slugs and the presence of pathogenic nematodes. This survey provides a first insight into the natural populations of mollusk-associated nematodes in the mid-Atlantic region. This knowledge may contribute to implementing cultural practices favoring these natural enemies of slug pests.
Exogenous Application of Indol-3-Acetic Acid and Salicylic Acid Improves Tolerance to Salt Stress in Olive Plantlets (Olea europaea L. Cultivar Picual) in Growth Chamber Environments
Authors: del Pilar Cordovilla María , Aparicio Carolina , Melendo Manuel , Bueno Milagros
Published: February 2023

Salinity is one the most recurrent abiotic stresses worldwide and severely affects crop productivity in arid and semiarid environments. This research analyzed several plant growth regulators that could mitigate the effects of salinity on olive plants (Olea europaea L. cultivar Picual). Mist-rooted cuttings were grown in a growth chamber and pretreated with gibberellic acid (GA3), indole-3-acetic acid (IAA), salicylic acid (SA), and Kinetin by foliar spraying twice a week for three weeks. At the end of the pretreatment, the plants were exposed to 100 mM and 200 mM sodium chloride (NaCl) for six weeks. The results showed that plants pretreated with the plant growth regulators significantly increased their biomass under saline conditions. In addition, IAA and SA restricted the transport of sodium (Na+) ions from roots to leaves and improved the leaf potassium (K+)/Na+ ratio. IAA and SA favored proline, fructose, and mannitol accumulation in leaves at 100 mM and 200 mM NaCl, as did glucose at 200 mM NaCl. Salicylic acid and IAA increased pigments (chlorophylls and carotenoids) and polyamines accumulation under saline conditions. The findings of this study suggest that pretreatments with IAA and SA may be a highly effective way of increasing salt tolerance in olive plantlets.
Comparative Nutrient and Antioxidant Profile of High Lycopene Variety with hp Genes and Ordinary Variety of Tomato under Organic Conditions
Authors: Romdhane Amani , Riahi Anissa , Ujj Apolka , Ramos-Diaz Fernanda , Marjanović Jana , Hdider Chafik
Published: February 2023

Organic tomato cultivation is growing all over the world due to its healthy functional properties and environmental concerns. Recently, some new varieties with increased functional properties, particularly high lycopene, have been developed. However, few were assessed under organic farming systems. The objective of this study was to evaluate physico-chemical properties and the main bioactive compound contents, as well as lipophilic, hydrophilic and total radical scavenging activities of a recently developed high lycopene tomato variety homozygous for hp-2dg genes (HLT-F71) grown under organic conditions for two non-consecutive years (2019 and 2021) compared to the ordinary control ‘Nemador’ variety. The lycopene, β-carotene, total phenol, flavonoid, vitamin C, radical scavenging activity and tocopherols were analyzed by using spectrophotometric and HPLC methods, respectively. The high lycopene content variety presented suitable marketable yield, average fruit weight, pH, titratable acidity, firmness and higher °Brix, pulp color than the control. Additionally, it has significantly higher lycopene, β-carotene, total phenol, flavonoid, vitamin C and particularly considerable α-tocopherol contents, as well as radical scavenging activity. The high lycopene tomato variety with hp genes proved to be an effective sustainable variety for enhancing tomato fruit yield and functional properties even under organic grown conditions.
Basic Farmland Protection System in China: Changes, Conflicts and Prospects
Authors: Wang Nan , Hao Jinmin , Zhang Lei , Duan Wenkai , Shi Yunyang , Zhang Jinyi , Wusimanjiang Paruke
Published: February 2023

Farmland protection is important for ensuring national food security and promoting sustainable socioeconomic development. China has a far lower amount of farmland area per capita than the global average. To improve farmland productivity, the Chinese government has implemented a basic farmland protection system (BFPS). A comprehensive and systematic analysis of the evolution mechanism of the BFPS, the failures of the BFPS and the key measures of the modern BFPS reform is lacking in the current literature. Based on a systematic review of the history of China’s BFPS, this study first summarizes the evolutionary mechanism of the BFPS, then analyzes the current conflicts faced by the BFPS on this basis, proposes several main breakthrough strategies for improving the BFPS and finally provides key ideas for further strengthening the development of the BFPS in the future. The results of this study show that China’s BFPS has gone through three development stages since 1963 and that there are differences in the main factors hindering the improvement in basic farmland productivity in the different stages. Correspondingly, the systems adopted to meet the demands for basic farmland protection are also different. The evolution of the BFPS is similar to a “scale” that constantly seeks balance between “system demand” and “system supply”. In the present stage, the main conflicts faced by China’s BFPS are between basic farmland quality and requirements for supplementary delimitation and production patterns; between basic farmland quantity, urban development and food security; and between basic farmland-use regulation and modern agriculture and the market economy. The Chinese government should further optimize the BFPS through improving the delimitation system, establishing a classified protection system and strengthening the basic farmland protection compensation system. To accelerate the establishment of a territorial spatial planning system in the future, the BFPS should also be fully integrated with the concept of an ecological civilization, be applied to resolve the contradiction between development and protection and be used to help improve the land-space-utilization control system, thus creating a unified development guide for national land.
National Modern Agricultural Industrial Parks: Development Characteristics, Regional Differences, and Experience Inspiration—Case Study of 200 NMAIPs in China
Authors: Ling Lisi , Chen Xueyuan , Wu Yongchang , Li Shanwei , Wei Jiajia , Zhou Qun
Published: February 2023

Agricultural industries are the foundation of the modernization of agricultural and rural areas in China. National Modern Agricultural Industrial Parks (NMAIPs) provides a considerable nationwide platform for agricultural industries. We take 200 NMAIPs in China as objects. Through spatial analysis, the Herfindahl–Hirschman index, and the SBM-DEA model, we analyzed the development characteristics and regional differences of NMAIPs from the muti-level perspective of national planning, provincial coordination, and county implementation to propose policy recommendations aimed at sustainable and high-quality development. The results are as follows: (1) Regarding geospatial characteristics, NMAIPs are unevenly distributed, with a decreasing gradient from east to west. The direction is east (northward) to west (southward), consistent with the direction of the Hu line. The distribution density shows that the east is dense and the west is sparse. (2) For industrial concentration, the leading industries in NMAIPs tend to be homogenous. The HHI indicates that the homogenization of leading industries is widely represented in each province. The low oligopolistic areas are in the central and eastern regions of China, while the highly oligopolistic locations are in the western and northeastern provinces. (3) In inputs–outputs efficiency, the comprehensive technical efficiency is high but not optimal, while the distribution of values is high in the south and low in the north. Ten provinces are non-effective. According to inputs and outputs, the ineffective contribution of population of townships covered, occupied area and the capital from the collective economy are development barriers, and the high output value of NMAIPs cannot fully drive the employment and income of farmers. Further improvements are needed in terms of both pure technical efficiency and scale efficiency, and adjustments to scale operations should be in response to different returns to scale. Our research results provide policy recommendations for NMAIPs, including the establishment of a multi-level management mechanism, balancing regional development, diversifying and coordinating regional leading industries, and improving the efficiency of utilization factors.
Non-Invasive Methods for Assessing the Welfare of Farmed White-Leg Shrimp (Penaeus vannamei)
Authors: Pedrazzani Ana Silvia , Cozer Nathieli , Quintiliano Murilo Henrique , Tavares Camila Prestes dos Santos , da Silva Ubiratã de Assis Teixeira , Ostrensky Antonio
Published: February 2023

Gradually, concern for the welfare of aquatic invertebrates produced on a commercial/industrial scale is crossing the boundaries of science and becoming a demand of other societal actors. The objective of this paper is to propose protocols for assessing the Penaeus vannamei welfare during the stages of reproduction, larval rearing, transport, and growing-out in earthen ponds and to discuss, based on a literature review, the processes and perspectives associated with the development and application of on-farm shrimp welfare protocols. Protocols were developed based on four of the five domains of animal welfare: nutrition, environment, health, and behaviour. The indicators related to the psychology domain were not considered a separate category, and the other proposed indicators indirectly assessed this domain. For each indicator, the corresponding reference values were defined based on literature and field experience, apart from the three possible scores related to animal experience on a continuum from positive (score 1) to very negative (score 3). It is very likely that non-invasive methods for measuring the farmed shrimp welfare, such as those proposed here, will become a standard tool for farms and laboratories and that it will become increasingly challenging to produce shrimp without considering their welfare throughout the production cycle.
M-Mode Echocardiographic Measurements of Interventricular Septum, Left Ventricular Internal Diameter, and Left Ventricular Free-Wall Thickness in Normal Horses—A Meta-Analytical Study
Authors: Marzok Mohamed , Kandeel Mahmoud , Babiker Hussein , Alkhodair Khalid M. , Farag Alshimaa , Ibrahim Hussam , El-Ashker Maged , Alghuwainem Yousef , El-khodery Sabry
Published: February 2023

The purpose of this study was to provide a systematic meta-analysis on echocardiographic measurements in normal Thoroughbred and Standardbred horses. The current systematic meta-analysis followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). All the available published papers on the reference values of echocardiographic assessment via M-mode echocardiography were searched, and fifteen studies were finally selected for analysis. In both fixed and random effect, the confidence interval (CI) for the interventricular septum (IVS) was 2.8–3.1 and 4.7–7.5; for the left ventricular free-wall (LVFW) thickness, it was 2.9–3.2 and 4.2–6.7; and for the left ventricular internal diameter (LVID), it was −5.0–4.6 and −10.0–−6.7, respectively. For IVS, the Q statistic, I-squared, and tau-squared were 925.3, 98.1, and 7.9, respectively. Similarly, for LVFW, all the effects were on the positive side of zero, with a range of 1.3–68.1. The CI indicated a significant variation among the studies (fixed, 2.9–3.2; random, 4.2–6.7). The z-values of LVFW for fixed and random effects were, respectively, 41.1 (p < 0.001) and 8.5 (p < 0.001). However, the Q statistic was 886.6 (p < 0.001). Moreover, the I-squared was 98.08, and the tau-squared was 6.6. By contrast, the effects of LVID fell on the negative side of zero, (2.8–83.9). The present meta-analysis provides an overview of the echocardiographic measurements of cardiac diameters in healthy Thoroughbred and Standardbred horses. The meta-analysis indicates variations in results among different studies. This result should be considered when evaluating a horse for heart disease and each case should be evaluated independently.
Identification of a Novel Idiopathic Epilepsy Risk Locus and a Variant in the CCDC85A Gene in the Dutch Partridge Dog
Authors: Beckers Evy , Bhatti Sofie F. M. , Van Poucke Mario , Polis Ingeborgh , Farnir Frédéric , Van Nieuwerburgh Filip , Mandigers Paul , Van Ham Luc , Peelman Luc , Broeckx Bart J. G.
Published: February 2023

(1) Idiopathic epilepsy (IE) is thought to have a genetic cause in several dog breeds. However, only two causal variants have been identified to date, and few risk loci are known. No genetic studies have been conducted on IE in the Dutch partridge dog (DPD), and little has been reported on the epileptic phenotype in this breed. (2) Owner-filled questionnaires and diagnostic investigations were used to characterize IE in the DPD. A genome-wide association study (GWAS) involving 16 cases and 43 controls was performed, followed by sequencing of the coding sequence and splice site regions of a candidate gene within the associated region. Subsequent whole-exome sequencing (WES) of one family (including one IE-affected dog, both parents, and an IE-free sibling) was performed. (3) IE in the DPD has a broad range in terms of age at onset, frequency, and duration of epileptic seizures. Most dogs showed focal epileptic seizures evolving into generalized seizures. A new risk locus on chromosome 12 (BICF2G630119560; praw = 4.4 × 10−7; padj = 0.043) was identified through GWAS. Sequencing of the GRIK2 candidate gene revealed no variants of interest. No WES variants were located within the associated GWAS region. However, a variant in CCDC85A (chromosome 10; XM_038680630.1: c.689C > T) was discovered, and dogs homozygous for the variant (T/T) had an increased risk of developing IE (OR: 6.0; 95% CI: 1.6–22.6). This variant was identified as likely pathogenic according to ACMG guidelines. (4) Further research is necessary before the risk locus or CCDC85A variant can be used for breeding decisions.
Warm Season Turfgrass Equine Sports Surfaces: An Experimental Comparison of the Independence of Simple Measurements Used for Surface Characterization
Authors: Blanco María Alejandra , Di Rado Facundo Nicolas , Peterson Michael (Mick)
Published: February 2023

Turfgrass in equine sports has clear advantages over other types of reinforcement but adds complexity to the management. This study investigates factors that influence the turfgrass’ surface performance and how the use of a drainage package and a geotextile reinforcement affect quantitative measurements of turfgrass. The measurements are made using affordable, lightweight testing tools that are readily available or easily constructed. Eight boxes with turfgrass over a mix of the arena with peat at a consistent depth were tested for volumetric moisture content (VMC %) with time–domain reflectometry (TDR), the rotational peak shear device (RPS), the impact test device (ITD), soil cone penetrometer (SCP), and the Going Stick (GS). Results obtained using TDR, RPS, ITD, SCP, and GS indicate that the presence of the geotextile and drainage package was mainly detected by VMC (%), SCP detected geotextile addition, and GS detected the interaction of geotextile × drainage package. Linear regression showed SCP and GS are related to geotextile and was positively correlated between them and negatively with VMC (%). The testing showed some limitations of these devices, mainly related to moisture content and sod composition, but the potential exists to utilize these devices for quality control as well as for the monitoring of maintenance of the surfaces when controlling the range of both VMC (%) and sod constitution.
Diversity of Underwater Vocalizations in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
Authors: Zhou Lu , Lei Jinhong , Zhai Xiaofei , Lu Ningning , Shi Haitao , Wang Jichao
Published: February 2023

Sound communication is important for underwater species. The wild population of the Chinese soft-shelled turtle (Pelodiscus sinensis) is listed as vulnerable. However, its vocalization, which can serve as the basis for ecological and evolutionary research, has not been studied. Here, we performed underwater recordings of 23 Chinese soft-shelled turtles of different ages and sexes and identified 720 underwater calls. The turtle calls were manually divided into 10 call types according to visual and aural inspection properties. The similarity test indicated that the manual division was reliable. We described the acoustic properties of the calls and the statistical analysis showed that the peak frequency of calls was significantly different between adult females and males, and also between subadults and adults. Similar to other aquatic turtles that prefer to live in deep water, Chinese soft-shelled turtles have a high vocal diversity and many harmonic calls, indicating that this highly aquatic species developed a variety of vocalizations to enhance their underwater communication, which helped them adapt to the complex and dim underwater environment. Furthermore, the turtles showed a tendency for vocalization to become more diverse with age.
Research Progress on Glycoprotein 5 of Porcine Reproductive and Respiratory Syndrome Virus
Authors: Luo Qin , Zheng Yajie , Zhang Hang , Yang Zhiyu , Sha Huiyang , Kong Weili , Zhao Mengmeng , Wang Nina
Published: February 2023

Porcine reproductive and respiratory syndrome (PRRS) is an acute, febrile, and highly contagious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). Glycoprotein 5 (GP5) is a glycosylated envelope protein encoded by the PRRSV ORF5, which has good immunogenicity and can induce the body to produce neutralizing antibodies. Therefore, study of GP5 protein is of great significance in the diagnosis, prevention, and control of PRRSV and the development of new vaccines. We reviewed GP5 protein genetic variation, immune function, interaction with viral protein and host proteins, induction of cell apoptosis, and stimulation of neutralizing antibodies. GP5 protein’s influence on virus replication and virulence, as well as its use as a target for viral detection and immunization are reviewed.
Effects of Replacing Fishmeal with the Mixture of Cottonseed Protein Concentrate and Clostridium autoethanogenum Protein on the Growth, Nutrient Utilization, Serum Biochemical Indices, Intestinal and Hepatopancreas Histology of Rainbow Trout (Oncorhynchus mykiss)
Authors: Huang Hongfei , Li Xiaoqin , Cao Kailin , Leng Xiangjun
Published: February 2023

The purpose of this study was to develop the potential of cottonseed protein concentrate (CPC) and Clostridium autoethanogenum protein (CAP) in the diet of rainbow trout (Oncorhynchus mykiss) by evaluating the effects of substituting fishmeal with a CPC and CAP mixture on growth performance, nutrient utilization, serum biochemical indices, intestinal and hepatopancreas histology. In a basal diet containing 200 g/kg fishmeal (Con), the mixture of CPC and CAP (1:1) was used to reduce dietary fishmeal to 150, 100, 50 and 0 g/kg, to form five diets with the same crude protein and crude lipid contents (CON, FM-15, FM-10, FM-5 and FM-0). Then, the five diets were fed to rainbow trout (35.00 ± 0.05 g) for 8 weeks. The weight gain (WG) and feed conversion ratio (FCR) of the five groups were 258.72%, 258.82%, 249.90%, 242.89%, 236.57%, and 1.19, 1.20, 1.24, 1.28, 1.31, respectively. FM-5 and FM-0 groups showed significantly lower WG and higher FCR than the CON group (p < 0.05). In terms of whole-body composition, such as moisture, crude ash, and crude protein, no significant difference was observed among all the groups (p > 0.05), except that significantly higher crude lipid content was detected in the FM-0 group than in the CON group (p < 0.05). In the FM-5 and FM-0 groups, protein efficiency, protein retention, intestinal protease activity and amylase activity were significantly lower than in the CON group (p < 0.05). Compared to the CON group, the serum contents of glucose and total cholesterol in the FM-0 group as well as MDA in the FM-5 and FM-0 groups were significantly increased, and catalase, superoxide dismutase, and total antioxidant capacity were decreased (p < 0.05). In intestine and hepatopancreas histology, the intestinal villus height in the FM-5 and FM-0 groups and villus width in the FM-0 group were decreased significantly (p < 0.05), while no significant difference in hepatopancreas morphology was observed among all the groups except that some vacuolization was observed in the FM-0 group (p > 0.05). In summary, the mixture of CPC and CAP can effectively replace 100 g/kg fishmeal in a diet containing 200 g/kg fishmeal without adverse effects on the growth performance, nutrient utilization, serum biochemical, or intestinal and hepatopancreas histology of rainbow trout.
Genomic Analysis of the Endangered Fonni’s Dog Breed: A Comparison of Genomic and Phenotypic Evaluation Scores
Authors: Cortellari Matteo , Bionda Arianna , Cocco Raffaella , Sechi Sara , Liotta Luigi , Crepaldi Paola
Published: February 2023

The Fonni’s dog is an ancient Sardinian breed for livestock and property guarding. In recent years, the number of new registrations to the breeding book has slumped and, thus, this breed risks being lost forever. This work refocuses attention to the Fonni’s dog, analysing its genomic makeup and comparing different phenotypical and genetic evaluation scores. Thirty Fonni’s dogs were ranked by their general accordance to the breed typicality (typicality score) and to the provisional standard by official judges (judges’ score). They were genotyped with a 230K SNP BeadChip and compared with 379 dogs of 24 breeds. Genomically, the Fonni’s dogs placed themselves near shepherd dogs and showed a unique genetic signature, which was used to create the genomic score. This score better correlated with typicality (ρ = 0.69, p < 0.0001) than the judges’ score (ρ = 0.63, p = 0.0004), which showed little variability among the included dogs. Hair texture or colour were significantly associated in the three scores. The Fonni’s dog is confirmed as a well-distinguished breed, despite being selected mainly for its work abilities. The evaluation criteria used during dog expositions can be improved to increase their variability and include elements typical of the breed. The recovery of the Fonni’s dog would be possible only with a shared vision between the Italian kennel club and breeders, and the support of regional programs.
Naked Mole-Rats Demonstrate Profound Tolerance to Low Oxygen, High Carbon Dioxide, and Chemical Pain
Authors: Amoroso Vince G. , Zhao Aishi , Vargas Isabel , Park Thomas J.
Published: February 2023

Naked mole-rats (Heterocephalus glaber) are very unusual among subterranean mammals in that they live in large colonies and are extremely social, spending large amounts of time gathered together in underground nests more than a meter below the surface. Many respiring individuals resting in deep, poorly ventilated nests deplete the oxygen supply and increase the concentration of carbon dioxide. Consistent with living in that atmosphere, naked mole-rats tolerate levels of low oxygen and high carbon dioxide that are deadly to most surface-dwelling mammals. Naked mole-rats appear to have evolved a number of remarkable adaptations to be able to thrive in this harsh atmosphere. In order to successfully survive low oxygen atmospheres, they conserve energy utilization by reducing the physiological activity of all organs, manifest by reduced heart rate and brain activity. Amazingly, they resort to the anaerobic metabolism of fructose rather than glucose as a fuel to generate energy when challenged by anoxia. Similarly, high carbon dioxide atmospheres normally cause tissue acidosis, while naked mole-rats have a genetic mutation preventing both acid-induced pain and pulmonary edema. Together, these putative adaptations and the tolerances they provide make the naked mole-rat an important model for studying a host of biomedical challenges.
Owners’ Beliefs regarding the Emotional Capabilities of Their Dogs and Cats
Authors: Pickersgill Olivia , Mills Daniel S. , Guo Kun
Published: February 2023

The correct interpretation of an animal’s emotional state is crucial for successful human–animal interaction. When studying dog and cat emotional expressions, a key source of information is the pet owner, given the extensive interactions they have had with their pets. In this online survey we asked 438 owners whether their dogs and/or cats could express 22 different primary and secondary emotions, and to indicate the behavioral cues they relied upon to identify those expressed emotions. Overall, more emotions were reported in dogs compared to cats, both from owners that owned just one species and those that owned both. Although owners reported a comparable set of sources of behavioral cues (e.g., body posture, facial expression, and head posture) for dogs and cats in expressing the same emotion, distinct combinations tended to be associated with specific emotions in both cats and dogs. Furthermore, the number of emotions reported by dog owners was positively correlated with their personal experience with dogs but negatively correlated with their professional experience. The number of emotions reported in cats was higher in cat-only households compared to those that also owned dogs. These results provide a fertile ground for further empirical investigation of the emotional expressions of dogs and cats, aimed at validating specific emotions in these species.