bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparisons between the Neighboring States of Amazonas and Pará in Brazil in the Second Wave of COVID-19 Outbreak and a Possible Role of Early Ambulatory Treatment
Authors: Emmerich Francisco G.
Published: March 2021

Brazil and many countries are now experiencing a second wave of the COVID-19 outbreak. The objective of this study is to compare results with statistical samples involving millions of people in the two largest neighboring states in Brazil, Amazonas and Pará, which in the first wave were similar but now show significant different results in combating COVID-19. During the first wave, in May 2020, the maximums of the 7-day average daily deaths per population of Amazonas and Pará were similar: 15.7 and 17.1 deaths per day per million people, respectively, which means a ratio 15.7/17.1 = 0.92 ≈ 1. Now, in the second wave of COVID-19 outbreak, Amazonas has entered a serious situation; meanwhile, Pará has presented a much smaller growth in the mortality. The accumulated mortality per population from 11 November 2020 to 15 March 2021 of Amazonas and Pará are 1645 and 296 deaths per million people, respectively. As 1645/296 = 5.55, Amazonas is presenting an accumulated mortality per population more than five times that of Pará. Future in-depth research can provide a grounded answer to explain this significant difference, nonetheless the explicit support of the Pará state government, after 21 May 2020, to early ambulatory treatment may have played some role on this result.
Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region
Authors: Nyabi Omar , Bentahir Mostafa , Ambroise Jérôme , Bearzatto Bertrand , Chibani Nawfal , Smits Benjamin , Durant Jean François , Vybornov Aleksandr , Thellin Olivier , El Moualij Benaissa , Gala Jean-Luc
Published: March 2021

Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed for the surveillance of COVID-19 cases by large-scale testing of first responders. The objective was to assess the seroconversion among the regional civil protection (CP), police, health care professionals, and volunteers. The secondary objective was to detect asymptomatic individuals within this cohort in the light of age, sex, and residence. In this paper, we report the results of serological testing performed by the B-LiFe mobile laboratory deployed from 10 June to 23 July 2020. The tests included whole blood finger-prick and serum sampling for detection of SARS-CoV-2 spike receptor-binding domain (S-RBD) antibodies. The prevalence of SARS-CoV-2 antibodies was approximately 5% (294/6013). The results of the finger-prick tests and serum sample analyses showed moderate agreement (kappa = 0.77). Furthermore, the detection rates of serum antibodies to the SARS-CoV-2 nucleocapsid protein (NP) and S-RBD among the seroconverted individuals were positively correlated (kappa = 0.60), at least at the IgG level. Seroprevalence studies based on serological testing for the S-RBD protein or SARS-CoV-2 NP antibodies are not sufficient for diagnosis but might help in screening the population to be vaccinated and in determining the duration of seroconversion.
Synthesis and Characterization of Na-Zeolites from Textile Waste Ash and Its Application for Removal of Lead (Pb) from Wastewater
Authors: Hussain Tabassum , Hussain Abdullah Ijaz , Chatha Shahzad Ali Shahid , Ali Adnan , Rizwan Muhammad , Ali Shafaqat , Ahamd Parvaiz , Wijaya Leonard , Alyemeni Mohammed Nasser
Published: March 2021

Massive production of carcinogenic fly ash waste poses severe threats to water bodies due to its disposal into drains and landfills. Fly ash can be a source of raw materials for the synthesis of adsorbents. Rag fly ash as a new class of raw materials could be a cheap source of Al and Si for the synthesis of Na-zeolites. In this work, NaOH activation, via a prefusion- and postfusion-based hydrothermal strategy, was practiced for the modification of rag fly ash into Na-zeolite. Morphology, surface porosity, chemical composition, functionality, mineral phases, and crystallinity, in conjunction with ion exchangeability of the tailored materials, were evaluated by SEM, ICP-OES, XRF, FTIR, XRD, and cation exchange capacity (CEC) techniques. Rag fly ash and the synthesized Na-zeolites were applied for the removal of Pb (II) from synthetic wastewater by varying the reaction conditions, such as initial metal ion concentration, mass of adsorbent, sorption time, and pH of the reaction medium. It was observed that Na-zeolite materials (1 g/100 mL) effectively removed up to 90–98% of Pb (II) ions from 100 mg/L synthetic solution within 30 min at pH ≈ 8. Freundlich adsorption isotherm favors the multilayer heterogeneous adsorption mechanism for the removal of Pb (II). It is reasonable to conclude that recycling of textile rag fly ash waste into value-added Na-zeolites for the treatment of industrial wastewater could be an emergent move toward achieving sustainable and green remediation.
Pain in Multiple Sites and Clusters of Cause-Specific Work Disability Development among Midlife Municipal Employees
Authors: Hiilamo Aapo , Huttu Anna , Øverland Simon , Pietiläinen Olli , Rahkonen Ossi , Lallukka Tea
Published: March 2021

This study investigates to what extent pain in multiple sites and common risk factors related to work environment, occupational class and health behaviours are associated with cause-specific work disability (WD) development clusters. The study population was derived from the Finnish Helsinki Health Study (n = 2878). Sequence analysis created clusters of similar subsequent cause-specific WD development in an eight-year follow-up period. Cross-tabulations and multinomial logistic regression were used to analyze the extent to which baseline factors, including pain in multiple sites, were associated with the subsequent WD clusters. A solution with five distinct WD clusters was chosen: absence of any WD (40%), low and temporary WD due to various causes (46%), WD due to mental disorders (3%), WD due to musculoskeletal (8%) and WD due to other causes (4%). Half of the employees in the musculoskeletal WD cluster had pain in multiple locations. In the adjusted model the number of pain sites, low occupational class and physical working conditions were linked to the musculoskeletal WD. The identified characteristics of the different WD clusters may help target tailored work disability prevention measures for those at risk.
Long Diagnostic Delay with Unknown Transmission Route Inversely Correlates with the Subsequent Doubling Time of Coronavirus Disease 2019 in Japan, February–March 2020
Authors: Ogata Tsuyoshi , Tanaka Hideo
Published: March 2021

Long diagnostic delays (LDDs) may decrease the effectiveness of patient isolation in reducing subsequent transmission of coronavirus disease 2019 (COVID-19). This study aims to investigate the correlation between the proportion of LDD of COVID-19 patients with unknown transmission routes and the subsequent doubling time. LDD was defined as the duration between COVID-19 symptom onset and confirmation ≥6 days. We investigated the geographic correlation between the LDD proportion among 369 confirmed COVID-19 patients with symptom onset between the 9th and 11th week and the subsequent doubling time for 717 patients in the 12th–13th week among the six prefectures. The doubling time on March 29 (the end of the 13th week) ranged from 4.67 days in Chiba to 22.2 days in Aichi. Using a Pearson’s product-moment correlation (p-value = 0.00182) and multiple regression analyses that were adjusted for sex and age (correlation coefficient −0.729, 95% confidence interval: −0.923–−0.535, p-value = 0.0179), the proportion of LDD for unknown exposure patients was correlated inversely with the base 10 logarithm of the subsequent doubling time. The LDD for unknown exposure patients was correlated significantly and inversely with the subsequent doubling time.
A Qualitative Study of Barriers and Enablers of Physical Activity among Female Emirati University Students
Authors: Burton Nicola W. , Barber Bonnie L. , Khan Asaduzzaman
Published: March 2021

Interventions to promote physical activity participation should reflect social and culturally relevant influences of the target demographic. The aim of this study was to explore perceptions of barriers to and enablers of physical activity participation among female Emirati university students. Five semi-structured focus groups were conducted (n = 25). Participants were asked open-ended questions about benefits, barriers and enablers of physical activity, and recommendations to promote participation. Emergent themes were identified using Nvivo software. Commonly identified benefits included improved health, weight management, improved mood, and stress reduction. The main barriers were low family support, competing time demands from domestic and academic activities, lack of convenient access to women-only facilities, and hot weather. The main enablers and recommendations related to social support from family and friends, accessible and low-cost women-only facilities, and structured supervised sessions. Findings suggest that there are specific social-cultural influences of physical activity among female Emirati university students. Approaches to promote participation could include identifying benefits consistent with family and cultural values, using social media for education, support and modelling, on campus supervised physical activity sessions integrated with the academic timetable, low-cost women-only opportunities in the local residential area, and support for home-based activities.
The Frequency of Use and Harm Perception of Heated Tobacco Products (HTPs): The 2019 Cross-Sectional Survey among Medical Students from Poland
Authors: Majek Paulina , Jankowski Mateusz , Nowak Bartłomiej , Macherski Maksymilian , Nowak Maciej , Gil Aleksandra , Nakiela Piotr , Lewicka Barbara , Lawson Joshua Allan , Zejda Jan Eugeniusz , Brożek Grzegorz Marek
Published: March 2021

Heated tobacco products (HTPs) are devices for generating a nicotine aerosol by heating the tobacco sticks. This study aimed to assess (1) the prevalence of HTP and tobacco cigarette usage among medical students, (2) to characterize smoking habits and (3) to assess students’ awareness and opinions about HTPs. A cross-sectional survey on the frequency and attitudes toward cigarettes, e-cigarettes and HTP use was performed between 2019–2020 at the Medical University of Silesia in Katowice (Poland). The data were obtained from 1344 students aged 21.8 ± 1.9 years (response rate: 66.9%). Current traditional tobacco use was 13.2%, e-cigarettes use 3.5%, and HTP use 2.8% of students. Duration of use was shorter among HTPs users comparing to cigarette smokers (p < 0.001) although the number of tobacco sticks used daily was similar (p = 0.1). Almost 30% of respondents have ever tried HTPs. HTPs were considered safe by 5.3% of respondents (43.2% of HTP users vs. 3.9% of non-HTP users, p < 0.001). HTP users were more likely to report that heating tobacco is not addictive (odds ratio (OR) = 8.9, 95% confidence interval (CI): 1.8–45.8) and disagreed with a public ban on HTP use (OR = 4.9, 95%CI: 2.5–9.8). Among students, HTP use was less popular than cigarette smoking, but awareness of their presence is widespread.
Light-Mediated Regulation of Leaf Senescence
Authors: Sakuraba Yasuhito
Published: March 2021

Light is the primary regulator of various biological processes during the plant life cycle. Although plants utilize photosynthetically active radiation to generate chemical energy, they possess several photoreceptors that perceive light of specific wavelengths and then induce wavelength-specific responses. Light is also one of the key determinants of the initiation of leaf senescence, the last stage of leaf development. As the leaf photosynthetic activity decreases during the senescence phase, chloroplasts generate a variety of light-mediated retrograde signals to alter the expression of nuclear genes. On the other hand, phytochrome B (phyB)-mediated red-light signaling inhibits the initiation of leaf senescence by repressing the phytochrome interacting factor (PIF)-mediated transcriptional regulatory network involved in leaf senescence. In recent years, significant progress has been made in the field of leaf senescence to elucidate the role of light in the regulation of nuclear gene expression at the molecular level during the senescence phase. This review presents a summary of the current knowledge of the molecular mechanisms underlying light-mediated regulation of leaf senescence.
Engaging the Innate and Adaptive Antitumor Immune Response in Lymphoma
Authors: Csizmar Clifford M. , Ansell Stephen M.
Published: March 2021

Immunotherapy has emerged as a powerful therapeutic strategy for many malignancies, including lymphoma. As in solid tumors, early clinical trials have revealed that immunotherapy is not equally efficacious across all lymphoma subtypes. For example, immune checkpoint inhibition has a higher overall response rate and leads to more durable outcomes in Hodgkin lymphomas compared to non-Hodgkin lymphomas. These observations, combined with a growing understanding of tumor biology, have implicated the tumor microenvironment as a major determinant of treatment response and prognosis. Interactions between lymphoma cells and their microenvironment facilitate several mechanisms that impair the antitumor immune response, including loss of major histocompatibility complexes, expression of immunosuppressive ligands, secretion of immunosuppressive cytokines, and the recruitment, expansion, and skewing of suppressive cell populations. Accordingly, treatments to overcome these barriers are being rapidly developed and translated into clinical trials. This review will discuss the mechanisms of immune evasion, current avenues for optimizing the antitumor immune response, clinical successes and failures of lymphoma immunotherapy, and outstanding hurdles that remain to be addressed.
Granulibacter bethesdensis, a Pathogen from Patients with Chronic Granulomatous Disease, Produces a Penta-Acylated Hypostimulatory Glycero-D-talo-oct-2-ulosonic Acid–Lipid A Glycolipid (Ko-Lipid A)
Authors: Muszyński Artur , Zarember Kol A. , Heiss Christian , Shiloach Joseph , Berg Lars J. , Audley John , Kozyr Arina , Greenberg David E. , Holland Steven M. , Malech Harry L. , Azadi Parastoo , Carlson Russell W. , Gallin John I.
Published: March 2021

Granulibacter bethesdensis can infect patients with chronic granulomatous disease, an immunodeficiency caused by reduced phagocyte NADPH oxidase function. Intact G. bethesdensis (Gb) is hypostimulatory compared to Escherichia coli, i.e., cytokine production in human blood requires 10–100 times more G. bethesdensis CFU/mL than E. coli. To better understand the pathogenicity of G. bethesdensis, we isolated its lipopolysaccharide (GbLPS) and characterized its lipid A. Unlike with typical Enterobacteriaceae, the release of presumptive Gb lipid A from its LPS required a strong acid. NMR and mass spectrometry demonstrated that the carbohydrate portion of the isolated glycolipid consists of α-Manp-(1→4)-β-GlcpN3N-(1→6)-α-GlcpN-(1⇿1)-α-GlcpA tetra-saccharide substituted with five acyl chains: the amide-linked N-3′ 14:0(3-OH), N-2′ 16:0(3-O16:0), and N-2 18:0(3-OH) and the ester-linked O-3 14:0(3-OH) and 16:0. The identification of glycero-d-talo-oct-2-ulosonic acid (Ko) as the first constituent of the core region of the LPS that is covalently attached to GlcpN3N of the lipid backbone may account for the acid resistance of GbLPS. In addition, the presence of Ko and only five acyl chains may explain the >10-fold lower proinflammatory potency of GbKo–lipidA compared to E. coli lipid A, as measured by cytokine induction in human blood. These unusual structural properties of the G.bethesdensis Ko–lipid A glycolipid likely contribute to immune evasion during pathogenesis and resistance to antimicrobial peptides.
Cytotoxic Activity of LLO Y406A Is Targeted to the Plasma Membrane of Cancer Urothelial Cells
Authors: Resnik Nataša , Tratnjek Larisa , Kreft Mateja Erdani , Kisovec Matic , Aden Saša , Bedina Zavec Apolonija , Anderluh Gregor , Podobnik Marjetka , Veranič Peter
Published: March 2021

Identification of novel agents for bladder cancer treatment is highly desirable due to the high incidence of tumor recurrence and the risk of progression to muscle-invasive disease. The key feature of the cholesterol-dependent toxin listeriolysin O mutant (LLO Y406A) is its preferential activity at pH 5.7, which could be exploited either directly for selective targeting of cancer cells or the release of accumulated therapeutics from acidic endosomes. Therefore, our goal was to compare the cytotoxic effect of LLO Y406A on cancer cells (RT4) and normal urothelial cells (NPU), and to identify which cell membranes are the primary target of LLO Y406A by viability assays, life-cell imaging, fluorescence, and electron microscopy. LLO Y406A decreased viability, altered cell morphology, provoked membrane blebbing, and induced apoptosis in RT4 cells, while it did not affect NPU cells. LLO Y406A did not cause endosomal escape in RT4 cells, while the plasma membrane of RT4 cells was revealed as the primary target of LLO Y406A. It has been concluded that LLO Y406A has the ability to selectively eliminate cancer urothelial cells through pore-forming activity at the plasma membrane, without cytotoxic effects on normal urothelial cells. This promising selective activity merits further testing as an anti-cancer agent.
Islet Regeneration: Endogenous and Exogenous Approaches
Authors: Docherty Fiona M. , Sussel Lori
Published: March 2021

Both type 1 and type 2 diabetes are characterized by a progressive loss of beta cell mass that contributes to impaired glucose homeostasis. Although an optimal treatment option would be to simply replace the lost cells, it is now well established that unlike many other organs, the adult pancreas has limited regenerative potential. For this reason, significant research efforts are focusing on methods to induce beta cell proliferation (replication of existing beta cells), promote beta cell formation from alternative endogenous cell sources (neogenesis), and/or generate beta cells from pluripotent stem cells. In this article, we will review (i) endogenous mechanisms of beta cell regeneration during steady state, stress and disease; (ii) efforts to stimulate endogenous regeneration and transdifferentiation; and (iii) exogenous methods of beta cell generation and transplantation.
Deciphering the Binding Interactions between Acinetobacter baumannii ACP and β-ketoacyl ACP Synthase III to Improve Antibiotic Targeting Using NMR Spectroscopy
Authors: Choi Sungjae , Park Jungwoo , Yeon Jiwon , Jang Ahjin , Lee Woo Cheol , Kim Yangmee
Published: March 2021

Fatty acid synthesis is essential for bacterial viability. Thus, fatty acid synthases (FASs) represent effective targets for antibiotics. Nevertheless, multidrug-resistant bacteria, including the human opportunistic bacteria, Acinetobacter baumannii, are emerging threats. Meanwhile, the FAS pathway of A. baumannii is relatively unexplored. Considering that acyl carrier protein (ACP) has an important role in the delivery of fatty acyl intermediates to other FAS enzymes, we elucidated the solution structure of A. baumannii ACP (AbACP) and, using NMR spectroscopy, investigated its interactions with β-ketoacyl ACP synthase III (AbKAS III), which initiates fatty acid elongation. The results show that AbACP comprises four helices, while Ca2+ reduces the electrostatic repulsion between acid residues, and the unconserved F47 plays a key role in thermal stability. Moreover, AbACP exhibits flexibility near the hydrophobic cavity entrance from D59 to T65, as well as in the α1α2 loop region. Further, F29 and A69 participate in slow exchanges, which may be related to shuttling of the growing acyl chain. Additionally, electrostatic interactions occur between the α2 and α3-helix of ACP and AbKAS III, while the hydrophobic interactions through the ACP α2-helix are seemingly important. Our study provides insights for development of potent antibiotics capable of inhibiting A. baumannii FAS protein–protein interactions.
Participation of ABCA1 Transporter in Pathogenesis of Chronic Obstructive Pulmonary Disease
Authors: Kotlyarov Stanislav
Published: March 2021

Chronic obstructive pulmonary disease (COPD) is the important medical and social problem. According to modern concepts, COPD is a chronic inflammatory disease, macrophages play a key role in its pathogenesis. Macrophages are heterogeneous in their functions, which is largely determined by their immunometabolic profile, as well as the features of lipid homeostasis, in which the ATP binding cassette transporter A1 (ABCA1) plays an essential role. The objective of this work is the analysis of the ABCA1 protein participation and the function of reverse cholesterol transport in the pathogenesis of COPD. The expression of the ABCA1 gene in lung tissues takes the second place after the liver, which indicates the important role of the carrier in lung function. The participation of the transporter in the development of COPD consists in provision of lipid metabolism, regulation of inflammation, phagocytosis, and apoptosis. Violation of the processes in which ABCA1 is involved may be a part of the pathophysiological mechanisms, leading to the formation of a heterogeneous clinical course of the disease.
Pseudomonas Flagella: Generalities and Specificities
Authors: Bouteiller Mathilde , Dupont Charly , Bourigault Yvann , Latour Xavier , Barbey Corinne , Konto-Ghiorghi Yoan , Merieau Annabelle
Published: March 2021

Flagella-driven motility is an important trait for bacterial colonization and virulence. Flagella rotate and propel bacteria in liquid or semi-liquid media to ensure such bacterial fitness. Bacterial flagella are composed of three parts: a membrane complex, a flexible-hook, and a flagellin filament. The most widely studied models in terms of the flagellar apparatus are E. coli and Salmonella. However, there are many differences between these enteric bacteria and the bacteria of the Pseudomonas genus. Enteric bacteria possess peritrichous flagella, in contrast to Pseudomonads, which possess polar flagella. In addition, flagellar gene expression in Pseudomonas is under a four-tiered regulatory circuit, whereas enteric bacteria express flagellar genes in a three-step manner. Here, we use knowledge of E. coli and Salmonella flagella to describe the general properties of flagella and then focus on the specificities of Pseudomonas flagella. After a description of flagellar structure, which is highly conserved among Gram-negative bacteria, we focus on the steps of flagellar assembly that differ between enteric and polar-flagellated bacteria. In addition, we summarize generalities concerning the fuel used for the production and rotation of the flagellar macromolecular complex. The last part summarizes known regulatory pathways and potential links with the type-six secretion system (T6SS).
Poly(chitosan-ester-ether-urethane) Hydrogels as Highly Controlled Genistein Release Systems
Authors: Zagórska-Dziok Martyna , Kleczkowska Patrycja , Olędzka Ewa , Figat Ramona , Sobczak Marcin
Published: March 2021

Polymeric hydrogels play an increasingly important role in medicine, pharmacy and cosmetology. They appear to be one of the most promising groups of biomaterials due to their favorable physicochemical properties and biocompatibility. The objective of the presented study was to synthesize new poly(chitosan-ester-ether-urethane) hydrogels and to study the kinetic release of genistein (GEN) from these biomaterials. In view of the above, six non-toxic hydrogels were synthesized via the Ring-Opening Polymerization (ROP) and polyaddition processes. The poly(ester-ether) components of the hydrogels have been produced in the presence of the enzyme as a biocatalyst. In some cases, the in vitro release rate of GEN from the obtained hydrogels was characterized by near-zero-order kinetics, without “burst release” and with non-Fickian transport. It is important to note that developed hydrogels have been shown to possess the desired safety profile due to lack of cytotoxicity to skin cells (keratinocytes and fibroblasts). Taking into account the non-toxicity of hydrogels and the relatively highly controlled release profile of GEN, these results may provide fresh insight into polymeric hydrogels as an effective dermatological and/or cosmetological tool.
System Design to Utilize Domain Expertise for Visual Exploratory Data Analysis
Authors: Langer Tristan , Meisen Tobias
Published: March 2021

Exploratory data analysis (EDA) is an iterative process where data scientists interact with data to extract information about their quality and shape as well as derive knowledge and new insights into the related domain of the dataset. However, data scientists are rarely experienced domain experts who have tangible knowledge about a domain. Integrating domain knowledge into the analytic process is a complex challenge that usually requires constant communication between data scientists and domain experts. For this reason, it is desirable to reuse the domain insights from exploratory analyses in similar use cases. With this objective in mind, we present a conceptual system design on how to extract domain expertise while performing EDA and utilize it to guide other data scientists in similar use cases. Our system design introduces two concepts, interaction storage and analysis context storage, to record user interaction and interesting data points during an exploratory analysis. For new use cases, it identifies historical interactions from similar use cases and facilitates the recorded data to construct candidate interaction sequences and predict their potential insight—i.e., the insight generated from performing the sequence. Based on these predictions, the system recommends the sequences with the highest predicted insight to data scientist. We implement a prototype to test the general feasibility of our system design and enable further research in this area. Within the prototype, we present an exemplary use case that demonstrates the usefulness of recommended interactions. Finally, we give a critical reflection of our first prototype and discuss research opportunities resulting from our system design.
Chromosome Unipolar Division and Low Expression of Tws May Cause Parthenogenesis of Rice Water Weevil (Lissorhoptrus oryzophilus Kuschel)
Authors: Wang Pengcheng , Yang Fangyuan , Ma Zhuo , Zhang Runzhi
Published: March 2021

Rice water weevil (RWW) is divided into two types of population, triploid parthenogenesis and diploid bisexual reproduction. In this study, we explored the meiosis of triploid parthenogenesis RWW (Shangzhuang Town, Haidian District, Beijing, China) by marking the chromosomes and microtubules of parthenogenetic RWW oocytes via immunostaining. The immunostaining results show that there is a canonical meiotic spindle formed in the triploid parthenogenetic RWW oocytes, but chromosomes segregate at only one pole, which means that there is a chromosomal unipolar division during the oogenesis of the parthenogenetic RWW. Furthermore, we cloned the conserved sequences of parthenogenetic RWW REC8 and Tws, and designed primers based on the parthenogenetic RWW sequence to detect expression patterns by quantitative PCR (Q-PCR). Q-PCR results indicate that the expression of REC8 and Tws in ovarian tissue of bisexual Drosophila melanogaster is 0.98 and 10,000.00 times parthenogenetic RWW, respectively (p < 0.01). The results show that Tws had low expression in parthenogenetic RWW ovarian tissue, and REC8 was expressed normally. Our study suggests that the chromosomal unipolar division and deletion of Tws may cause parthenogenesis in RWW.
The Role of Cardiac Magnetic Resonance in Evaluation of Idiopathic Ventricular Arrhythmia in Children
Authors: Kamińska Halszka , Małek Łukasz A. , Barczuk-Falęcka Marzena , Bartoszek Marta , Strzałkowska-Kominiak Ewa , Marszałek Mikołaj , Brzezik Ewa , Brzewski Michał , Werner Bożena
Published: March 2021

The aim of the study was to assess the role of cardiovascular magnetic resonance (CMR) in the diagnosis of idiopathic VA in children. This retrospective single-centre study included a total of 80 patients with idiopathic ventricular arrhythmia that underwent routine CMR imaging between 2016 and 2020 at our institution. All patients underwent a 3.0 T scan involving balanced steady-state free precession cine images as well as dark-blood T2W images and assessment of late gadolinium enhancement (LGE). In 26% of patients (n = 21) CMR revealed cardiac abnormalities, in 20% (n = 16) not suspected on prior echocardiography. The main findings included: non-ischemic ventricular scars (n = 8), arrhythmogenic right ventricular cardiomyopathy (n = 6), left ventricular clefts (n = 4) and active myocarditis (n = 3). LGE was present in 57% of patients with abnormal findings. Univariate predictors of abnormal CMR result included abnormalities in echocardiography and severe VA (combination of >10% of 24 h VA burden and/or presence of ventricular tachycardia and/or polymorphic VA). CMR provides valuable clinical information in many cases of idiopathic ventricular arrhythmia in children, mainly due to its advanced tissue characterization capabilities and potential to assess the right ventricle.
Impact of COVID-19 at the Ocular Level: A Citation Network Study
Authors: Sánchez-Tena Miguel Ángel , Martinez-Perez Clara , Villa-Collar Cesar , Alvarez-Peregrina Cristina
Published: March 2021

Background: The main objective of this study was to use citation networks to analyze the relationship between different publications on the impact of COVID-19 at an ocular level and their authors. Furthermore, the different research areas will be identified, and the most cited publication will be determined. Materials and Methods: The publications were searched within the Web of Science database, using “ocular”, “SARS-CoV-2”, “ophthalmology”, “eyesight”, and “COVID-19” as keywords for the period between January 2020 and January 2021. The Citation Network Explorer and the CiteSpace software were used to analyze the different publications. Results: A total of 389 publications with 890 citations generated on the web were found. It must be highlighted that July was the month with the largest number of publications. The most cited ones were “Characteristics of Ocular Findings of Patients with Coronavirus Disease 2019 (COVID-19) in Hubei Province, China” by Wu et al., which was published in May 2020. Three groups covering the different research areas in this field were found using the clustering functions: ocular manifestations, teleophthalmology, and personal protective equipment. Conclusions: The citation network has shown a comprehensive and objective analysis of the main studies on the impact of COVID-19 in ocular disease.
The Influence of Probiotic Supplementation on Depressive Symptoms, Inflammation, and Oxidative Stress Parameters and Fecal Microbiota in Patients with Depression Depending on Metabolic Syndrome Comorbidity—PRO-DEMET Randomized Study Protocol
Authors: Gawlik-Kotelnicka Oliwia , Skowrońska Anna , Margulska Aleksandra , Czarnecka-Chrebelska Karolina H. , Łoniewski Igor , Skonieczna-Żydecka Karolina , Strzelecki Dominik
Published: March 2021

There is a huge need to search for new treatment options and potential biomarkers of therapeutic response to antidepressant treatment. Depression and metabolic syndrome often coexist, while a pathophysiological overlap, including microbiota changes, may play a role. The paper presents a study protocol that aims to assess the effect of probiotic supplementation on symptoms of depression, anxiety and stress, metabolic parameters, inflammatory and oxidative stress markers, as well as fecal microbiota in adult patients with depressive disorders depending on the co-occurrence of metabolic syndrome. The trial will be a four-arm, parallel-group, prospective, randomized, double-blind, controlled design that will include 200 participants and will last 20 weeks (ClinicalTrials.gov identifier: NCT04756544). The probiotic preparation will contain Lactobacillus helveticus Rosell®-52, Bifidobacterium longum Rosell®-175. We will assess the level of depression, anxiety and stress, quality of life, blood pressure, body mass index and waist circumference, white blood cells count, serum levels of C-reactive protein, high-density lipoprotein (HDL) cholesterol, triglycerides, fasting glucose, fecal microbiota composition and the level of some fecal microbiota metabolites, as well as serum inflammatory markers and oxidative stress parameters. The proposed trial may establish a safe and easy-to-use adjunctive treatment option in a subpopulation of depressive patients only partially responsive to pharmacologic therapy.
Porous Media Computational Fluid Dynamics and the Role of the First Coil in the Embolization of Ruptured Intracranial Aneurysms
Authors: Wiśniewski Karol , Tomasik Bartłomiej , Tyfa Zbigniew , Reorowicz Piotr , Bobeff Ernest J. , Stefańczyk Ludomir , Posmyk Bartłomiej J. , Jóźwik Krzysztof , Jaskólski Dariusz J.
Published: March 2021

Background: The objective of our project was to identify a late recanalization predictor in ruptured intracranial aneurysms treated with coil embolization. This goal was achieved by means of a statistical analysis followed by a computational fluid dynamics (CFD) with porous media modelling approach. Porous media CFD simulated the hemodynamics within the aneurysmal dome after coiling. Methods: Firstly, a retrospective single center analysis of 66 aneurysmal subarachnoid hemorrhage patients was conducted. The authors assessed morphometric parameters, packing density, first coil volume packing density (1st VPD) and recanalization rate on digital subtraction angiograms (DSA). The effectiveness of initial endovascular treatment was visually determined using the modified Raymond–Roy classification directly after the embolization and in a 6- and 12-month follow-up DSA. In the next step, a comparison between porous media CFD analyses and our statistical results was performed. A geometry used during numerical simulations based on a patient-specific anatomy, where the aneurysm dome was modelled as a separate, porous domain. To evaluate hemodynamic changes, CFD was utilized for a control case (without any porosity) and for a wide range of porosities that resembled 1–30% of VPD. Numerical analyses were performed in Ansys CFX solver. Results: A multivariate analysis showed that 1st VPD affected the late recanalization rate (p < 0.001). Its value was significantly greater in all patients without recanalization (p < 0.001). Receiver operating characteristic curves governed by the univariate analysis showed that the model for late recanalization prediction based on 1st VPD (AUC 0.94 (95%CI: 0.86–1.00) is the most important predictor of late recanalization (p < 0.001). A cut-off point of 10.56% (sensitivity—0.722; specificity—0.979) was confirmed as optimal in a computational fluid dynamics analysis. The CFD results indicate that pressure at the aneurysm wall and residual flow volume (blood volume with mean fluid velocity > 0.01 m/s) within the aneurysmal dome tended to asymptotically decrease when VPD exceeded 10%. Conclusions: High 1st VPD decreases the late recanalization rate in ruptured intracranial aneurysms treated with coil embolization (according to our statistical results > 10.56%). We present an easy intraoperatively calculable predictor which has the potential to be used in clinical practice as a tip to improve clinical outcomes.
Statically Analyzing the Energy Efficiency of Software Product Lines
Authors: Couto Marco , Fernandes João Paulo , Saraiva João
Published: March 2021

Optimizing software to become (more) energy efficient is an important concern for the software industry. Although several techniques have been proposed to measure energy consumption within software engineering, little work has specifically addressed Software Product Lines (SPLs). SPLs are a widely used software development approach, where the core concept is to study the systematic development of products that can be deployed in a variable way, e.g., to include different features for different clients. The traditional approach for measuring energy consumption in SPLs is to generate and individually measure all products, which, given their large number, is impractical. We present a technique, implemented in a tool, to statically estimate the worst-case energy consumption for SPLs. The goal is to reason about energy consumption in all products of a SPL, without having to individually analyze each product. Our technique combines static analysis and worst-case prediction with energy consumption analysis, in order to analyze products in a feature-sensitive manner: a feature that is used in several products is analyzed only once, while the energy consumption is estimated once per product. This paper describes not only our previous work on worst-case prediction, for comprehensibility, but also a significant extension of such work. This extension has been realized in two different axis: firstly, we incorporated in our methodology a simulated annealing algorithm to improve our worst-case energy consumption estimation. Secondly, we evaluated our new approach in four real-world SPLs, containing a total of 99 software products. Our new results show that our technique is able to estimate the worst-case energy consumption with a mean error percentage of 17.3% and standard deviation of 11.2%.
Microbiome Status of Cider-Apples, from Orchard to Processing, with a Special Focus on Penicillium expansum Occurrence and Patulin Contamination
Authors: Al Riachy Reem , Strub Caroline , Durand Noël , Guibert Benjamin , Guichard Hugues , Constancias Florentin , Chochois Vincent , Lopez-Lauri Félicie , Fontana Angélique , Schorr-Galindo Sabine
Published: March 2021

Patulin is a secondary metabolite produced primarily by the fungus Penicillium expansum, responsible for the blue mold disease on apples. It is found in apple products including apple cider when apple juice is added after fermentation. In the present study, two hundred and twenty-five cider-apples of the variety “Bedan”, cultivated in Brittany in France, were sampled from the orchard during harvesting until the storage step, right before processing. The patulin analysis on these samples reported a low contamination at the orchard and a significantly higher-level of contamination in the cider-apples starting from the transporting bin. The percentage of positive samples increased from 6% to 47% after 12 h in the harvesting bin before transporting and reached 95% after 24 h of transporting, decreasing then to 69% at the end of the storage. Penicillium expansum was quantified on the surface of apples using real-time PCR and was observed to be mostly consistent between the harvest and post-harvest steps. It was detected on average, on the surface of 85% of all sampled apples with a mean value around 2.35 × 106Penicillium expansum DNA/g of apple. Moreover, the changes in the fungal and bacterial epiphytic microbiota in the different steps were studied using a metabarcoding approach. The alpha and beta diversity analysis revealed the presence of unique and more diverse bacterial and fungal communities on the surface of apples picked from the orchard compared to the rest of the sampling steps. Potential indigenous biological control agents were identified on the surface of sampled apples. Future perspective includes developing actions of prevention and control of the contamination by Penicillium expansum during the harvest and along the various critical post-harvest stages before transformation in a sustainable development concern.
RETRACTED: Protecting the Rights of Minorities under International Law and Implications of COVID-19: An Overview of the Indian Context
Authors: Ahmad Nehaluddin
Published: March 2021

The concept of majority rule and respect for minority rights is demonstrated in several constitutions of the world. Oppression by the majority of the minority is barred by articles of these respective constitutions. Today, democracy is mostly a method of government of the people that is ruled by the people. The issue of minority rights is at the center of the concept of civic rights. Minority protection, thus, operates on the hypothesis that religious, cultural, and linguistic affiliations are essential features of the very notion of a civic, just society. This paper offers an alternative account of why minority rights have international significance and more information on the value of an international, socially just process for the allocation of resources by states. By this approach, international minority rights speak to the wrongs that international law itself produces by organizing international political reality into a legal order. This article focuses on the uncertain effect of religious autonomy in India and the outcome of democracy in the country. While the Indian constitution guarantees autonomy to its religious minorities and promises minorities their freedoms, Indian democracy, which was once considered remarkable in scale and duration, has been weakened by the rise of xenophobic nationalism and threats to religious minorities. Even the safety and religious freedom of minorities have been compromised during COVID-19. In the last few decades, these trends have been clear; however, they have dramatically increased in the last few years, and the administration has turned a blind eye.
Combined Peridynamics and Discrete Multiphysics to Study the Effects of Air Voids and Freeze-Thaw on the Mechanical Properties of Asphalt
Authors: Sanfilippo Danilo , Ghiassi Bahman , Alexiadis Alessio , Hernandez Alvaro Garcia
Published: March 2021

This paper demonstrates the use of peridynamics and discrete multiphysics to assess micro crack formation and propagation in asphalt at low temperatures and under freezing conditions. Three scenarios are investigated: (a) asphalt without air voids under compressive load, (b) asphalt with air voids and (c) voids filled with freezing water. The first two are computed with Peridynamics, the third with peridynamics combined with discrete multiphysics. The results show that the presence of voids changes the way cracks propagate in the material. In asphalt without voids, cracks tend to propagate at the interface between the mastic and the aggregate. In the presence of voids, they ‘jump’ from one void to the closest void. Water expansion is modelled by coupling Peridynamics with repulsive forces in the context of Discrete Multiphysics. Freezing water expands against the voids’ internal surface, building tension in the material. A network of cracks forms in the asphalt, weakening its mechanical properties. The proposed methodology provides a computational tool for generating samples of ‘digital asphalt’ that can be tested to assess the asphalt properties under different operating conditions.
Formation of Die Soldering and the Influence of Alloying Elements on the Intermetallic Interface
Authors: Kohlhepp Marius , Uggowitzer Peter J. , Hummel Marc , Höppel Heinz Werner
Published: March 2021

Die soldering of die castings is a serious problem in the aluminum casting industry. The precise mechanism, the influence of the alloy composition, and the options for prevention have not yet been fully elaborated. A well-established solution for alloys with low iron content is the addition of manganese. However, up to 0.8 wt.% is necessary, which increases the amount of brittle phases in the material and consequently reduces ductility. Immersion tests with 1.2343 tool steel and pure aluminum as well as a hypoeutectic AlSi-alloy with Mn, Mo, Co, and Cr additions were carried out to systematically investigate the formation of die soldering. Three different intermetallic layers and a scattered granular intermetallic phase formed at the interface between steel and Al-alloy after immersion into the melt for a duration of 6 min at 710 °C. The combined presence of the irregular, needle-shaped β-Al5FeSi phase and the surrounding alloy was responsible for the bond between the two components. Mn and Mo inhibited the formation of the β-phase, and instead promoted the αC-Al15(Fe,X)3Si2 phase. This led to an evenly running boundary to the AlSi-alloy and thus prevented bonding. Cr has proven to be the most efficient addition against die soldering, with 0.2 wt.% being sufficient. Contrary to the other elements investigated, Cr also reduced the thickness of the intermetallic interface.
Glass-Forming Ability and Corrosion Resistance of Al88Y8−xFe4+x (x = 0, 1, 2 at.%) Alloys
Authors: Babilas Rafał , Spilka Monika , Młynarek Katarzyna , Łoński Wojciech , Łukowiec Dariusz , Radoń Adrian , Kądziołka-Gaweł Mariola , Gębara Piotr
Published: March 2021

The effect of iron and yttrium additions on glass forming ability and corrosion resistance of Al88Y8-xFe4+x (x = 0, 1, 2 at.%) alloys in the form of ingots and melt-spun ribbons was investigated. The crystalline multiphase structure of ingots and amorphous-crystalline structure of ribbons were examined by a number of analytical techniques including X-ray diffraction, Mössbauer spectroscopy, and transmission electron microscopy. It was confirmed that the higher Fe additions contributed to formation of amorphous structures. The impact of chemical composition and structure of alloys on their corrosion resistance was characterized by electrochemical tests in 3.5% NaCl solution at 25 °C. The identification of the mechanism of chemical reactions taking place during polarization test along with the morphology and internal structure of the surface oxide films generated was performed. It was revealed that the best corrosion resistance was achieved for the Al88Y7Fe5 alloy in the form of ribbon, which exhibited the lowest corrosion current density (jcorr = 0.09 μA/cm2) and the highest polarization resistance (Rp = 96.7 kΩ∙cm2).
Using Specified Risk Materials-Based Peptides for Oil Sands Fluid Fine Tailings Management
Authors: Zhu Yeling , Gong Yuki , Kaminsky Heather , Chae Michael , Mussone Paolo , Bressler David C.
Published: March 2021

Fluid fine tailings are produced in huge quantities by Canada’s mined oil sands industry. Due to the high colloidal stability of the contained fine solids, settling of fluid fine tailings can take hundreds of years, making the entrapped water unavailable and posing challenges to public health and the environment. This study focuses on developing value-added aggregation agents from specified risk materials (SRM), a waste protein stream from slaughterhouse industries, to achieve an improved separation of fluid fine tailings into free water and solids. Settling results using synthetic kaolinite slurries demonstrated that, though not as effective as hydrolyzed polyacrylamide, a commercial flocculant, the use of SRM-derived peptides enabled a 2-3-fold faster initial settling rate than the blank control. The pH of synthetic kaolinite tailings was observed to be slightly reduced with increasing peptides dosage in the test range (10–50 kg/ton). The experiments on diluted fluid fine tailings (as a representation of real oil sands tailings) demonstrated an optimum peptides dosage of 14 kg/ton, which resulted in a 4-fold faster initial settling rate compared to the untreated tailings. Overall, this study demonstrates the novelty and feasibility of using SRM-peptides to address intractable oil sands fluid tailings.