bluuebunny/crossref_metadata_2025 huggingface dataset:
Cognitive aging at work and in daily life—a narrative review on challenges due to age-related changes in central cognitive functions
Authors: Getzmann Stephan , Reiser Julian E. , Gajewski Patrick D. , Schneider Daniel , Karthaus Melanie , Wascher Edmund
Published: August 2023

Demographic change is leading to an increasing proportion of older employees in the labor market. At the same time, work activities are becoming more and more complex and require a high degree of flexibility, adaptability, and cognitive performance. Cognitive control mechanism, which is subject to age-related changes and is important in numerous everyday and work activities, plays a special role. Executive functions with its core functions updating, shifting, and inhibition comprises cognitive control mechanisms that serve to plan, coordinate, and achieve higher-level goals especially in inexperienced and conflicting actions. In this review, influences of age-related changes in cognitive control are demonstrated with reference to work and real-life activities, in which the selection of an information or response in the presence of competing but task-irrelevant stimuli or responses is particularly required. These activities comprise the understanding of spoken language under difficult listening conditions, dual-task walking, car driving in critical traffic situations, and coping with work interruptions. Mechanisms for compensating age-related limitations in cognitive control and their neurophysiological correlates are discussed with a focus on EEG measures. The examples illustrate how to access influences of age and cognitive control on and in everyday and work activities, focusing on its functional role for the work ability and well-being of older people.
Impact of United States refugee ban and discrimination on the mental health of hypertensive Arabic-speaking refugees
Authors: Albahsahli Behnan , Bridi Lana , Aljenabi Raghad , Abu-Baker Dania , Kaki Dahlia A. , Godino Job G. , Al-Rousan Tala
Published: August 2023

Background

Hypertension is a global leading cause of death which disproportionately affects refugees. This chronic disease increases the risk of heart disease, stroke, brain, and other end-organ disease, if left uncontrolled. The 2017 United States travel or “Muslim” ban prevented immigrants and refugees from seven Muslim-majority countries from entering the United States, including Syria and Iraq; two major contributors to the global refugee population. As of 2020, the United States has admitted more than 133,000 and 22,000 Iraqi and Syrian refugees, respectively. Studies on the health effects of this policy on refugees are lacking. This study qualitatively explores the impact of the refugee ban on United States resettled Syrian and Iraqi refugees with hypertension.

Methods

Participants were recruited through a federally qualified health center system that is the largest healthcare provider for refugees in San Diego, CA. All participants were Arabic-speaking refugees diagnosed with hypertension from Syria and Iraq. In-depth interviews took place between April 2021 and April 2022. Inductive thematic analysis was used to analyze data from semi-structured interviews.

Results

Participants (N = 109) include 53 women and 56 men (23 Syrian, 86 Iraqi). The average age was 61.3 years (SD: 9.7) and stay in the United States was 9.5 years (SD 5.92). Four themes emerged linking the travel ban’s impact on health, in line with the society to cells framework: (1) family factors: the refugee ban resulted in family separation; (2) physiological factors: the refugee ban worsened participants’ mental health, exacerbating hypertension and perceived health outcomes; (3) community factors: perpetuation of Islamophobia, xenophobia, and perceived discrimination were structural barriers with links to poorer health; and (4) individual factors: trickle down consequences led to worsened participant self-image and self-perception within their host community.

Discussion

The refugee ban negatively impacted the mental and physical health of United States resettled Arabic-speaking refugees through perceived discrimination, stress, and poor social integration. It continues to have long-lasting effects years after the ban was instated. Centering family reunification within the United States Refugee Admissions Program and tailoring interventions through the healthcare and public health systems are warranted to reduce hypertension disparities in this growing and overlooked population.
Implementation of an embedded behavioral health care model in a pediatric rheumatology subspecialty juvenile myositis clinic
Authors: Shenoi Susan , Edison Suzanne E. , Haynes Stacey , Patten Joanna
Published: August 2023

Youth with chronic medical illness, such as juvenile myositis (JM), require specialized behavioral health care. However, access to such care is challenging due to the youth mental health crisis, which impacts accessibility of mental health services in the community, as well as challenges accessing behavioral health care above and beyond the demands of care related to their JM management. In this paper we describe an embedded behavioral health care model, including the establishment and implementation of such a model, at a pediatric hospital where youth with JM receive medical care in a Center of Excellence (CoE). We describe a unique partnership with a philanthropic organization; the challenges and benefits of delivering care within this model; as well as recommendations for maximizing its effectiveness. Ultimately, we provide an example of a successful embedded behavioral health care program for youth with rare disease, which may be applied to other institutions providing similar care.
Differences in resting-state brain networks and gray matter between APOE ε2 and APOE ε4 carriers in non-dementia elderly
Authors: Wang Zhiyuan , Pang Jing , Zhou Ruizhi , Qi Jianjiao , Shi Xianglong , Han Bin , Man Xu , Wang Qingqing , Sun Jinping
Published: August 2023

Background

Apolipoprotein E (APOE) ε2 and APOE ε4 are the most distinct alleles among the three APOE alleles, both structurally and functionally. However, differences in cognition, brain function, and brain structure between the two alleles have not been comprehensively reported in the literature, especially in non-demented elderly individuals.

Methods

A neuropsychological test battery was used to evaluate the differences in cognitive performance in five cognitive domains. Independent component analysis (ICA) and voxel-based morphometry (VBM) were used separately to analyze resting-state functional magnetic resonance imaging (rs-fMRI) data and the structure MRI data between the two groups. Finally, correlations between differential brain regions and neuropsychological tests were calculated.

Results

APOE ε2 carriers had better cognitive performance in general cognitive, memory, attention, and executive function than APOE ε4 carriers (all p < 0.05). In ICA analyses of rs-fMRI data, the difference in the resting-state functional connectivity (rsFC) between two groups is shown in 7 brain networks. In addition, VBM analyses of the T1-weighted image revealed that APOE ε2 carriers had a larger thalamus and right postcentral gyrus volume and a smaller bilateral putamen volume than APOE ε4 carriers. Finally, differences in brain function and structure may be the reason that APOE ε2 carriers are better than APOE ε4 carriers in cognitive performance.

Conclusion

These findings suggest that there are significant differences in brain function and structure between APOE ε2 carriers and APOE ε4 carriers, and these significant differences are closely related to their cognitive performance.
Development and reliability testing of an audit toolbox for the assessment of the physical activity friendliness of urban and rural environments in Germany
Authors: Müller Christina , Domokos Bruno , Amersbach Tanja , Hausmayer Eva-Maria , Roßmann Christin , Wallmann-Sperlich Birgit , Bucksch Jens
Published: August 2023

Background

According to socio-ecological theories, physical activity behaviors are linked to the physical and social neighborhood environment. Reliable and contextually adapted instruments are needed to assess environmental characteristics related to physical activity. This work aims to develop an audit toolbox adapted to the German context, to urban and rural settings, for different population groups, and different types of physical activity; and to evaluate its inter-rater reliability.

Methods

We conducted a systematic literature search to collect existing audit tools and to identify the latest evidence of environmental factors influencing physical activity in general, as well as in German populations. The results guided the construction of a category system for the toolbox. Items were assigned to the categories based on their relevance to physical activity and to the German context as well as their comprehensibility. We piloted the toolbox in different urban and rural areas (100 street segments, 15 parks, and 21 playgrounds) and calculated inter-rater reliability by Cohen's Kappa.

Results

The audit toolbox comprises a basic streetscape audit with seven categories (land use and destinations, traffic safety, pedestrian infrastructure, cycling infrastructure, attractiveness, social environment, and subjective assessment), as well as supplementary tools for children and adolescents, seniors and people with impaired mobility, parks and public open spaces, playgrounds, and rural areas. 76 % of all included items had moderate, substantial, or almost perfect inter-rater reliability (κ > 0.4).

Conclusions

The audit toolbox is an innovative and reliable instrument for the assessment of the physical activity friendliness of urban and rural environments in Germany.
Citizen science for monitoring the health and well-being related Sustainable Development Goals and the World Health Organization’s Triple Billion Targets
Authors: Fraisl Dilek , See Linda , Estevez Diana , Tomaska Nola , MacFeely Steve
Published: August 2023

Achieving the health and well-being related Sustainable Development Goals (SDGs) and the World Health Organization’s (WHO) Triple Billion Targets depends on informed decisions that are based on concerted data collection and monitoring efforts. Even though data availability has been increasing in recent years, significant gaps still remain for routine surveillance to guide policies and actions. The COVID-19 crisis has shown that more and better data and strengthened health information systems are needed to inform timely decisions that save lives. Traditional sources of data such as nationally representative surveys are not adequate for addressing this challenge alone. Additionally, the funding required to measure all health and well-being related SDG indicators and Triple Billion Targets using only traditional sources of data is a challenge to achieving efficient, timely and reliable monitoring systems. Citizen science, public participation in scientific research and knowledge production, can contribute to addressing some of these data gaps efficiently and sustainably when designed well, and ultimately, could contribute to the achievement of the health and well-being related SDGs and Triple Billion Targets. Through a systematic review of health and well-being related indicators, as well as citizen science initiatives, this paper aims to explore the potential of citizen science for monitoring health and well-being and for mobilizing action toward the achievement of health and well-being related targets as outlined in the SDG framework and Triple Billion Targets. The results demonstrate that out of 58 health and well-being related indicators of the SDGs and Triple Billion Targets covered in this study, citizen science could potentially contribute to monitoring 48 of these indicators and their targets, mostly at a local and community level, which can then be upscaled at a national level with the projection to reach global level monitoring and implementation. To integrate citizen science with official health and well-being statistics, the main recommendation is to build trusted partnerships with key stakeholders including National Statistical Offices, governments, academia and the custodian agencies, which is mostly the WHO for these health and well-being related targets and indicators.
Adaptive restraint design for a diverse population through machine learning
Authors: Sun Wenbo , Liu Jiacheng , Hu Jingwen , Jin Judy , Siasoco Kevin , Zhou Rongrong , Mccoy Robert
Published: August 2023

Objective

Using population-based simulations and machine-learning algorithms to develop an adaptive restraint system that accounts for occupant anthropometry variations to further enhance safety balance throughout the whole population.

Methods

Two thousand MADYMO full frontal impact crash simulations at 35 mph using two validated vehicle/restraint models representing a sedan and an SUV along with a parametric occupant model were conducted based on the maximal projection design of experiments, which considers varying occupant covariates (sex, stature, and body mass index) and vehicle restraint design variables (three for airbag, three for safety belt, and one for knee bolster). A Gaussian-process-based surrogate model was trained to rapidly predict occupant injury risks and the associated uncertainties. An optimization framework was formulated to seek the optimal adaptive restraint design policy that minimizes the population injury risk across a wide range of occupant sizes and shapes while maintaining a low difference in injury risks among different occupant subgroups. The effectiveness of the proposed method was tested by comparing the population-wise injury risks under the adaptive design policy and the traditional state-of-the-art design.

Results

Compared to the traditional state-of-the-art design for midsize males, the optimal design policy shows the potential to further reduce the joint injury risk (combining head, chest, and lower extremity injury risks) among the whole population in the sedan and SUV models. Specifically, the two subgroups of vulnerable occupants including tall obese males and short obese females had higher reductions in injury risks.

Conclusions

This study lays out a method to adaptively adjust vehicle restraint systems to improve safety balance. This is the first study where population-based crash simulations and machine-learning methods are used to optimize adaptive restraint designs for a diverse population. Nevertheless, this study shows the high injury risks associated with obese and female occupants, which can be mitigated via restraint adaptability.
Progress in biological and medical research in the deep underground: an update
Authors: Zou Yuhao , Wang Ling , Wen Jirui , Cheng Juan , Li Can , Hao Zhizhen , Zou Jian , Gao Mingzhong , Li Weimin , Wu Jiang , Xie Heping , Liu Jifeng
Published: August 2023

As the growing population of individuals residing or working in deep underground spaces for prolonged periods, it has become imperative to understand the influence of factors in the deep underground environment (DUGE) on living systems. Heping Xie has conceptualized the concept of deep underground medicine to identify factors in the DUGE that can have either detrimental or beneficial effects on human health. Over the past few years, an increasing number of studies have explored the molecular mechanisms that underlie the biological impacts of factors in the DUGE on model organisms and humans. Here, we present a summary of the present landscape of biological and medical research conducted in deep underground laboratories and propose promising avenues for future investigations in this field. Most research demonstrates that low background radiation can trigger a stress response and affect the growth, organelles, oxidative stress, defense capacity, and metabolism of cells. Studies show that residing and/or working in the DUGE has detrimental effects on human health. Employees working in deep mines suffer from intense discomfort caused by high temperature and humidity, which increase with depth, and experience fatigue and sleep disturbance. The negative impacts of the DUGE on human health may be induced by changes in the metabolism of specific amino acids; however, the cellular pathways remain to be elucidated. Biological and medical research must continue in deep underground laboratories and mines to guarantee the safe probing of uncharted depths as humans utilize the deep underground space.
Stress granules: stress-induced cytoplasmic mRNPs compartments linked to mRNA translational regulatory pathways
Authors: Adjibade Pauline , Mazroui Rachid
Published: August 2023

Stress granules (SG) are macro-complexes that assemble as phase-separated and dynamic RNA biocondensates in the cytoplasm of the eukaryotic cell when the initiation step of the general translation of mRNAs is stalled. This occurs mainly as an adaptive cell response to either environmental (i.e., radiation, exposure to chemical drugs), pathological (i.e., viral treatment), physiological (i.e., oxygen-, amino acids-, and glucose-deprivation), or therapeutic (i.e., treatment with anti-cancer drugs) translational stress. SG also formed when translation initiation is blocked through stress-independent events including alteration of the activities of specific translation initiation factors and RNA-binding proteins. Both stress-dependent and–independent inhibition of translation initiation results in the accumulation of untranslated mRNAs, considered as integral components of SG. Consistently, in vivo assays of SG assembly combined with in vitro-based assembly of SG-like biocondensates studies support a fundamental role of the accumulation of untranslated mRNA in initiating the formation of SG, which then further promote their repression, potentially in a feed-back regulatory mechanism. The potential role of SG in actively repressing translation of associated mRNAs has been supported by a number of functional studies, establishing SG as critical regulatory sites of RNA homeostasis, in particular during stress. The view that the SG environment restricts translation of associated mRNAs was however challenged in studies showing that stress-induced translation repression can occur similarly in absence and presence of SG, leading to the emerging concept that formation of SG and translation repression are uncoupled processes. While it still a debate if mRNA recruitment to SG contributes to their translation repression, recent finding reported translation of reporter mRNAs in SG, suggesting rather an active translational role of SG. In this review, we describe the main translational signaling pathways that regulate the biology of SG, summarize current data supporting RNA as an integral functional component of SG, and then discuss evidence supporting or not the role of SG in regulating translation either negatively or positively during stress.
Transitioning toward sustainable consumption at the Swedish local governance level
Authors: Axelsson Katarina , André Karin , Dawkins Elena , Gerger Swartling Åsa , Xylia Maria
Published: August 2023

Municipalities have a prominent role to play in the transition to the sustainable society by governing changes at the local level. Based on a quantitative survey of Swedish municipalities, this study has given us a broad perspective on barriers and enablers in Swedish municipalities' efforts to plan, develop, and implement measures for governing the transition to sustainable consumption. By using a classification of municipalities belonging to certain groups, we find that municipalities characterized by having a city at their cores seem to have progressed further in their work to address sustainable consumption than municipalities characterized as more rural or as commuting municipalities near cities or towns. We also find that, though a large share of municipalities in Sweden report working systematically on sustainable consumption, their potential appears to not be fully realized, limited primarily by a lack of political support and key resources. We identify opportunities to establish more responsive governance structures as important for addressing these issues, where interactions at multiple levels are required to achieve successful governance of Sweden's work on sustainable consumption and address the barriers identified by this study. Higher levels of governance should offer greater support and guidance to municipalities in their work. We also advocate for more robust collaborations between municipalities to prevent them from working in isolation, build capacity, and foster greater knowledge exchange between municipality groups. This would strengthen municipalities' ability to catalyze transformational change, which is crucial if they are to meet their own high ambitions related to sustainable consumption and help institute the changes needed to enable the fulfillment of the long-term sustainability challenges we face, such as those articulated in the Paris Agreement and the 2030 Agenda for Sustainable Development.
Efficient galaxy classification through pretraining
Authors: Schneider Jesse , Stenning David C. , Elliott Lloyd T.
Published: August 2023

Deep learning has increasingly been applied to supervised learning tasks in astronomy, such as classifying images of galaxies based on their apparent shape (i.e., galaxy morphology classification) to gain insight regarding the evolution of galaxies. In this work, we examine the effect of pretraining on the performance of the classical AlexNet convolutional neural network (CNN) in classifying images of 14,034 galaxies from the Sloan Digital Sky Survey Data Release 4. Pretraining involves designing and training CNNs on large labeled image datasets unrelated to astronomy, which takes advantage of the vast amounts of such data available compared to the relatively small amount of labeled galaxy images. We show a statistically significant benefit of using pretraining, both in terms of improved overall classification success and reduced computational cost to achieve such performance.
Pharmacokinetic modelling of orally administered cannabidiol and implications for medication control in horses
Authors: Eichler Fabienne , Poźniak Błażej , Machnik Marc , Schenk Ina , Wingender Anke , Baudisch Natalie , Thevis Mario , Bäumer Wolfgang , Lischer Christoph , Ehrle Anna
Published: August 2023

Cannabidiol (CBD) products gain increasing popularity amongst animal owners and veterinarians as an alternative remedy for treatment of stress, inflammation or pain in horses. Whilst the use of cannabinoids is banned in equine sports, there is limited information available concerning CBD detection times in blood or urine. The aim of this study was to determine the pharmacokinetic properties of CBD following oral administration in the horse to assist doping control laboratories with interpreting CBD analytical results. Part 1: dose escalation study: Single oral administration of three escalating doses of CBD paste (0.2 mg/kg, n = 3 horses; 1 mg/kg, n = 3; 3 mg/kg, n = 5) with >7 days wash-out periods in between. Part 2: multiple dose study: oral administration of CBD paste (3 mg/kg, n = 6) twice daily for 15 days. Multiple blood and urine samples were collected daily throughout both studies. Following study part 2, blood and urine samples were collected for 2 weeks to observe the elimination phase. Concentrations of CBD, its metabolites and further cannabinoids were evaluated using gas-chromatography/tandem-mass-spectrometry. Pharmacokinetic parameters were assessed via two approaches: population pharmacokinetic analysis using a nonlinear mixed-effects model and non-compartmental analysis. AUC0–12 h and Cmax were tested for dose proportionality. During the elimination phase, the CBD steady-state urine to serum concentration ratio (Rss) was calculated. Oral CBD medication was well-tolerated in horses. Based on population pharmacokinetics, a three-compartment model with zero-order absorption most accurately described the pharmacokinetic properties of CBD. High volumes of distribution into peripheral compartments and high concentrations of 7-carboxy-CBD were observed in serum. Non-compartmental analysis identified a Cmax of 12.17 ± 2.08 ng/mL after single administration of CBD (dose: 3 mg/kg). AUC0–12 h showed dose proportionality, increase for Cmax leveled off at higher doses. Following multiple doses, the CBD terminal half-life was 161.29 ± 43.65 h in serum. Rss was 4.45 ± 1.04. CBD is extensively metabolized and shows high volumes of tissue distribution with a resulting extended elimination phase. Further investigation of the potential calming and anti-inflammatory effects of CBD are required to determine cut-off values for medication control using the calculated Rss.
A Bionic Control Method for Human–Exoskeleton Coupling Based on CPG Model
Authors: Sun Tianyi , Zhang Shujun , Li Ruiqi , Yan Yao
Published: August 2023

Exoskeleton robots are functioning in contexts with more complicated motion control needs as a result of the technology and applications for these robots rapidly developing. This calls for novel control techniques to accommodate their employment in a range of real-world settings. This paper proposes a bionic control method for a human–exoskeleton coupling dynamic model based on the CPG model, utilizing a model on the dynamics of the human–exoskeleton interaction. The CPG network is established as an oscillator by two neurons inhibiting one another, which approximates the torques simulated in the inverse dynamic analysis as the input to the exoskeleton robot. The findings of the simulation assessment suggest that the bionic control strategy may improve the robot’s ability to move quickly and steadily, as well as better adapt to challenging environments.
Translating and Validating the Frugality Scale among the Czech Population
Authors: Remr Jiri
Published: August 2023

Frugality is an important psychological trait that is currently of interest as a research construct in a range of fields, from consumer behavior to financial literacy and financial well-being. Increasingly, the concept of frugality is also being linked to environmental responsibility and behavior, as the core of frugality is the reduction or minimization of resources used and consumed, an emphasis on the long-term use of purchased products, and an overall conservation of resources. For many years, researchers have used the Frugality Scale (FS), the specific research tool introduced to measure frugality in a standardized and valid way. The aim of the study was to examine the psychometric properties of FS translated into the Czech language, to evaluate the uni-dimensionality of the construct, and to analyze associations with relevant variables documenting respondents’ attitudes and behavior. For this purpose, the research based on face-to-face interviews among respondents representing the 15–74 years old population of Czechia was conducted. The obtained results showed that the previously developed FS achieved very good results in the Czech environment, where the obtained scores supported the hypothesized uni-dimensional structure of the scale. The CFA results show that the tested model fits well with empirical data. Convergent and construct validity is also shown to be high. Therefore, the Czech version of the Frugality Scale can be considered a reliable and valid instrument that is recommended for further use. By utilizing the FS, researchers and practitioners gain access to a robust tool for quantifying frugality and comprehending its pertinent aspects across diverse contexts.
Where Do Social Support and Epistemic Centrality Come From? The Case of Innovators in the French Biotech Industry
Authors: Pina Stranger Alvaro , Varas German , Gerard Valentin
Published: August 2023

The link between entrepreneur’s network centrality and innovation performance has been broadly studied in knowledge-intensive industries such as biotechnology. However, little research has been focused on the social mechanisms that allow innovators to reach such a central position. We contribute to the existing literature by exploring the factors that may lead or prevent entrepreneurs from reaching a central position in their professional networks of knowledge exchange and social support in French biotech milieu. We use a unique quantitative and qualitative database of 138 and 126 biotech entrepreneurs observed, respectively, in 2008 and 2013. When accounting for entrepreneurs’ position in the social (friendship) and knowledge (advice) domain, we draw on three dimensions through which entrepreneurs build their position: their professional experience, their inter-organizational (or political) engagement, and the financial and geographical situation of their company. Results from a regression analysis showed that the specific individual and organizational aspects of the trajectory of the entrepreneurs explain their position in the observed networks. Factors such as the previous experience in the health industry, the training expertise, the international experience, the political engagement, and the geographical and financial situation of the company help entrepreneurs to build up their centrality. The two observations allow us to describe indirectly the evolution of norms that are considered legitimated to carry out innovation in the biotech field.
Experimental Investigation of Helicopter Noise While Approaching an Elevated Helipad
Authors: Gibertini Giuseppe , Rezzonico Silvano , Rossetti Marco , Zanotti Alex
Published: August 2023

The present paper describes a test campaign performed to investigate the noise footprint emitted by a helicopter in an idealised urban context, reproducing the approach to an elevated helipad. The test campaign was performed in Politecnico di Milano’s anechoic chamber and was finalised to investigate the effects produced only by helicopter noise. The set up consisted of a two-blade main rotor helicopter model and an aluminium rectangular prism model reproducing the landing building. Ground observer perceptions were recorded by means of a surface microphone and a realistic landing trajectory was approximated as a succession of fixed point measurements. Collected data were analysed through acoustic spectra and sound maps. Spectra were used to comprehend physical phenomena, such as reflection, diffraction and shielding, and to analyse the different contributions of helicopter noise. A sound map analysis enabled us to obtain a global perspective of the involved phenomena and to understand th extent to which people close to a building are stressed by a helicopter approaching an elevated urban helipad. Moreover, the experimental database, obtained over a free geometry, can be considered a useful tool for the validation of aeroacoustic solvers with different levels of fidelity.
Effectiveness of Information Acquisition via the Internet in Standardizing the Use of Antimicrobials by Hog Farmers: Insights from China
Authors: Si Ruishi , Yao Yumeng , Liu Mingyue
Published: August 2023

Antimicrobial residues and resistance caused by farmers’ overuse of veterinary antimicrobials have seriously threatened food safety, the ecological environment, and public health. With the popularization of the Internet in rural areas, especially in developing countries, the constraints of obtaining agricultural technical information provided by governments or organizations are greatly eased, farmers’ knowledge and skills are significantly improved, and the agricultural standardized production system is effectively constructed. However, there is still a research gap on whether information acquisition via the Internet (IAI) can induce farmers to standardize the use of antimicrobials. Using the data of 675 hog farmers in the Hebei, Shandong, Henan, and Hubei provinces, China, the IV-Heckman and mediating effect models were used to analyze the phenomenon empirically. The main findings revealed that the IAI had exerted a significant influence on the standardized use of veterinary antimicrobials by hog farmers, i.e., the IAI not only helped farmers to decide to standardize the use of antimicrobials but also reduced the amount of investment in the standardized use of antibiotics. Moreover, information-sharing and feedback mechanisms partially mediated the relationship between the IAI and farmers’ standardized use of antimicrobials. Finally, considering the heterogeneity of individual endowments, the study further revealed that the IAI significantly impacted the standardized use of antimicrobials for farmers below the age of 36 years. However, the IAI was found to positively and significantly promote farmers’ standardized-use decisions only if they had less than five years of breeding time.
Impact of Sea Rice Planting on Enzymatic Activity and Microbial Community of Coastal Soils: Focus on Proteinase
Authors: Yang Jie , Liu Zhiyun , Zhang Mingyi , Zhu Xiaolong , Wang Mingyi , Xu Xingfeng , Liu Guangchao
Published: August 2023

Soil proteinase and proteinase-producing microbial community are closely associated with soil fertility and soil health. Sea rice has been planted in the coastal beach of Jiaozhou Bay, China, in an effort to transform saline-alkali soil into arable land. However, the knowledge regarding the bacterial degradation of organic nitrogen in sea rice soils is limited. This study aims to investigate the physicochemical characteristics and enzymatic activities of the sea rice soils, as well as the microbial communities by both the Illumina sequencing-based culture-independent technology and culture-dependent methods. Sea rice soils exhibited a lower salinity and higher organic matter content and proteinase activity, as well as an increase in both the richness and diversity of the proteinase-producing bacterial community, compared to the adjacent non-rice soils. The Proteobacteria phylum and the Gammaproteobacteria class were dominant in sea rice soils, showing higher abundance than in the reference soils. The Planococcus genus and Bacillus-like bacterial communities were abundant in the cultivable proteinase-producing bacteria isolated from sea rice soils. Furthermore, a significant proportion of the extracellular proteinase produced by the isolated soil bacteria consisted of serine proteinases and metalloproteinases. These findings provided new insights into the degradation of soil organic nitrogen in coastal agricultural regions.
Effects of Combined Application of Biological Agent and Fertilizer on Fungal Community Structure in Rhizosphere Soil of Panax notoginseng
Authors: Liu Yanwei , Zhou Yingjie , Zhang Xiaofan , Cao Ni , Li Bin , Liang Jiaping , Yang Qiliang
Published: August 2023

The fungal community structure and soil fertility in rhizosphere soil have an important effect on the health of Panax notoginseng (P. notoginseng). The attack of pathogenic fungi and the imbalance of soil fertility can easily lead to diseases. The effect of Bacillus subtilis on improving the community structure of soil fungi has been confirmed, and the corresponding biological agent products have been commercialized. A pot experiment carried out in a greenhouse explored the effect of a biological agent and fertilizer on the fungal community in the rhizosphere of P. notoginseng. In the experiment, fertilization and the addition of biological agents were set up with three gradients, respectively, and the full coupling experiment was adopted, and the blank control group (CK) was set up at the same time. Therefore, there were thirteen treatments in the experiment. NH4 decreased between 36.42% and 11.56%, AP increased between 6.03% and 92.46%, AK increased between 2.99% and 25.40%, TN increased between 0.10% and 9.41%, and TP increased by 18.25% to 47.73% The addition of Bacillus subtilis biological agent decreased the Chao1, Shannon, Simpson, and ACE index of fungi in the rhizosphere soil of P. notoginseng. The Chao1 index decreased between 0.39% and 78.22%; the ACE index decreased between 0.43% and 78.24%. The main pathogenic fungi Cylindrocarpon and Fusarium of P. notoginseng were different in the experimental results. Cylindrocarpon decreased under F1C1, F2C1, and F3C2 treatments, while Fusarium increased under F1C1, F2C2, F3C1, and F3C3 treatments and decreased Fusarium content in rhizosphere soil of P. notoginseng in other treatments. RDA analysis (Redundancy analysis) showed that NH4-N was negatively correlated with the main pathogen Cylindrocarpon, Fusarium, and Ilyonectria, while AP and AK were positively correlated with Cylindrocarpon, Fusarium, and Ilyonectria. The results of the GRA-TOPSIS analysis showed that the score of F3C2 was the highest, while F2C3 and F2C1 ranked second and third, respectively. The calculation results of the theoretical model based on GRA-TOPSIS analysis showed that the GRA-TOPSIS score was highest when the theoretical optimal fertilizer application rate and bacteria application rate were 116.31 kg hm−2 and 15.83 kg hm−2, respectively.
Impact of Heat Stress on Blood, Production, and Physiological Indicators in Heat-Tolerant and Heat-Sensitive Dairy Cows
Authors: Chen Xiaoyang , Shu Hang , Sun Fuyu , Yao Junhu , Gu Xianhong
Published: August 2023

Heat stress affects production and health in cows severely. Since it is difficult to define heat-tolerant animals, studies of response to heat stress are important for understanding dairy cows’ health and production. However, information on the impact of heat stress on various indicators in heat-tolerant and heat-sensitive cows is sparse. This study aimed to investigate the effects of heat stress (HS) on blood, production, and physiological indicators in heat-tolerant and heat-sensitive cows. A total of 43 dairy cows were used from 9 May to 7 August 2021, under Temperature–Humidity Index (THI) measurements that ranged from 65.9 to 86.7. We identified cows that were tolerant or sensitive to HS based on the slope of the response of physiological and production traits against THI during the HS period by using a clustering method. After HS, serum glucose (Glu), cortisol (COR), 5-hydroxytryptamine (5-HT), and interleukin-6 (IL-6) levels of cows in the heat-tolerant group were lower than in the heat-sensitive group (p < 0.05). With THI as the predictor, the R2 for predicting respiration rate (RR) and body surface temperature (BT) in heat-tolerant cows was 0.15 and 0.16, respectively, whereas the R2 for predicting RR and BT in heat-sensitive cows was 0.19 and 0.18, respectively. There were low to moderate, positive correlations between RR, BT, and MY with THI, with Pearson correlation coefficients ranging from r = 0.11 to 0.4 in the heat-tolerant group, and from r = 0.24 to 0.43 in the heat-sensitive group. There was a low positive correlation between VT and THI, with a Spearman correlation coefficient of r = 0.07 in the heat-sensitive group. The heat-tolerant dairy cows had lower MY losses and had lower MY (p = 0.0007) in mixed models. Heat-tolerant cows with low-stress levels, through upregulating RR rapidly, increased their adaptability to thermal environments. They have better thermoregulation capability; the hypothalamic–pituitary–adrenal (HPA) axis regulated the thermoregulatory in animals by releasing a variety of neurotransmitters and hormones.
Equine Gunshot Euthanasia: Creation of a 3D-Printed Model with Integrated Sensors for Training
Authors: Dybdal Noël , Horgan Molly , Costa Lais , Davis Eric , Lucero Steven , Nieves Samantha , Quiroz Valerie , Weberg Kirsten , Madigan John E.
Published: August 2023

Challenges and issues related to the use of pentobarbital euthanasia and disposal of animal remains within the US have recently been reviewed. Environmental and public health challenges increasingly necessitate consideration of alternative methods such as gunshots, an American Veterinary Medical Association (AVMA) “acceptable with conditions” method, for the humane euthanasia of horses. A recent study reported a correctly aimed gunshot provides a humane option for euthanizing horses. However, although aiming guidelines exist, studies examining bullet trajectories in animals euthanized by gunshot have reported that inadequate disruption of the brain is a serious welfare issue. Here, we report the development and production of a portable, reusable, equine gunshot euthanasia training model. Using 3D printing, an anatomically accurate model of an equine head has been developed, with external aiming landmarks and equipped with integrated laser sensors and LED eyes. The laser sensors are embedded in two specific anatomical tracts (pons and medulla) with aiming paths associated with the aiming landmarks to train correct aiming angle. The LED eyes are linked to the laser sensors to provide instant feedback on aiming accuracy. When a beam from a commercially available blue training gun laser travels along the correct aiming path and strikes the sensor inside the head, the lights in the model’s eyes go out and there is an audible signal, providing immediate feedback on the accuracy of the shot. The model facilitates the training of veterinary personnel and first responders in successful gunshot euthanasia, providing instantaneous feedback on the likelihood of a shot causing immediate, humane death in a live animal.
Associations between Medical Disorders and Racing Outcomes in Poorly Performing Standardbred Trotter Racehorses: A Retrospective Study
Authors: Lo Feudo Chiara Maria , Stucchi Luca , Stancari Giovanni , Conturba Bianca , Bozzola Chiara , Zucca Enrica , Ferrucci Francesco
Published: August 2023

Poor performance in racehorses is commonly associated with subclinical diseases. This study aims to evaluate the associations between medical disorders and racing results in Standardbred trotters. The clinical records of 248 poorly performing Standardbreds were retrospectively reviewed, and their racing results were extracted from an online database, concerning the periods 3 months before and 6 months after hospitalization and the entire lifetime. Generalized linear models were used to evaluate the effects of different disorders on racing outcomes. Airway neutrophilia was associated with limiting lifetime starts and wins pre- and post-hospitalization, while mastocytosis was associated with less wins in the post-hospitalization period. Therefore, lower airway inflammation showed both short- and long-term impacts on racing performance. Severe upper airway obstructions and gastric ulcers showed associations with less placings in the post-discharge period but no long-term influence on performance. The significance of exertional rhabdomyolysis was indeterminable, yet interference with the number of starts in the post-discharge period was reported and associated with lower total career earnings. Exercise-induced pulmonary hemorrhage and cardiac arrhythmias were not associated with worse racing outcomes: therefore, their role in poor performance remains unclear.
Regulations on the Use of Antibiotics in Livestock Production in South America: A Comparative Literature Analysis
Authors: Da Silva Rafael Almeida , Arenas Nelson Enrique , Luiza Vera Lucia , Bermudez Jorge Antonio Zepeda , Clarke Sian E.
Published: August 2023

As a global health problem, antimicrobial resistance (AMR) crosses national borders, leading UN (United Nations) multilateral agencies to call for all countries to improve the stewardship of antibiotics in humans and animals. South American countries have changed their regulations regarding antibiotic use in livestock production. This literature review examines how far the five largest meat-producing countries in South America (Argentina, Brazil, Chile, Colombia, and Uruguay) have come in terms of the relevant legislation. Rules on market entry (marketing authorization and official distribution systems) are already set in all countries examined. Four countries do not allow growth promoters based on critically important antibiotics, and countries have also begun to set guidelines and minimum welfare and biosecurity requirements to reduce the therapeutic demand for antibiotics. Nonetheless, there are aspects related to the distribution, use, and disposal of antibiotics that need to be developed further. In conclusion, legislation in South American countries is moving towards the goals set by UN multilateral agencies, but more can be done. Differences between countries’ rules and the gold standards set by the World Organization for Animal Health (OIE), World Health Organization (WHO), and Food and Agriculture Organization of the United Nations (FAO) reveal possible adaptations to the countries’ realities. Further studies must examine compliance with the legislation already set and investigate other tools that can be used alongside legislation as a driving force to change stakeholder behaviour.
Integrative Transcriptomic Analysis Reveals Upregulated Apoptotic Signaling in Wound-Healing Pathway in Rat Liver Fibrosis Models
Authors: Kim Jihyun , Lee Changyong , Noh Sang Gyun , Kim Seungwoo , Chung Hae Young , Lee Haeseung , Moon Jeon-Ok
Published: August 2023

Liver fibrosis, defined by the aberrant accumulation of extracellular matrix proteins in liver tissue due to chronic inflammation, represents a pressing global health issue. In this study, we investigated the transcriptomic signatures of three independent liver fibrosis models induced by bile duct ligation, carbon tetrachloride, and dimethylnitrosamine (DMN) to unravel the pathological mechanisms underlying hepatic fibrosis. We observed significant changes in gene expression linked to key characteristics of liver fibrosis, with a distinctive correlation to the burn-wound-healing pathway. Building on these transcriptomic insights, we further probed the p53 signaling pathways within the DMN-induced rat liver fibrosis model, utilizing western blot analysis. We observed a pronounced elevation in p53 protein levels and heightened ratios of BAX/BCL2, cleaved/pro-CASPASE-3, and cleaved/full length-PARP in the livers of DMN-exposed rats. Furthermore, we discovered that orally administering oligonol—a polyphenol, derived from lychee, with anti-oxidative properties—effectively countered the overexpressions of pivotal apoptotic genes within these fibrotic models. In conclusion, our findings offer an in-depth understanding of the molecular alterations contributing to liver fibrosis, spotlighting the essential role of the apoptosis pathway tied to the burn-wound-healing process. Most importantly, our research proposes that regulating this pathway, specifically the balance of apoptosis, could serve as a potential therapeutic approach for treating liver fibrosis.
Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis
Authors: Merkel Melanie , Goebel Bjarne , Boll Moritz , Adhikari Aasha , Maurer Viktoria , Steinhilber Dieter , Culmsee Carsten
Published: August 2023

Ferroptosis is a form of oxidative cell death that is characterized by enhanced lipid peroxidation and mitochondrial impairment. The enzymes acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase (LPCAT) play an essential role in the biosynthesis of polyunsaturated fatty acid (PUFA)-containing phospholipids, thereby providing the substrates for lipid peroxidation and promoting ferroptosis. To examine the impact of mitochondria in ACSL4/LPCAT2-driven ferroptosis, HEK293T cells overexpressing ACSL4 and LPCAT2 (OE) or empty vector controls (LV) were exposed to 1S, 3R-RSL3 (RSL3) for induction of ferroptosis. The ACSL4/LPCAT2 overexpression resulted in higher sensitivity against RSL3-induced cell death compared to LV-transfected controls. Moreover, mitochondrial parameters such as mitochondrial reactive oxygen species (ROS) formation, mitochondrial membrane potential, and mitochondrial respiration deteriorated in the OE cells, supporting the conclusion that mitochondria play a significant role in ACSL4/LPCAT2-driven ferroptosis. This was further confirmed through the protection of OE cells against RSL3-mediated cell death by the mitochondrial ROS scavenger mitoquinone (MitoQ), which exerted protection via antioxidative properties rather than through previously reported metabolic effects. Our findings implicate that mitochondrial ROS production and the accompanying organelle disintegration are essential for mediating oxidative cell death initiated through lipid peroxidation in ferroptosis.