bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating crop growth between hydroponics and aquaponics with different light inputs
Authors: Heo Jeongwook , Baek Jeonghyun , Subah Zarin , Ryu Jae Hyeon
Published: July 2024

Indoor farming technologies, such as aquaponics and hydroponics, are crucial for advancing sustainable agriculture, especially in urban areas with limited land for conventional agriculture. As studies comparing these two systems are limited, this study explored the cultivation of five leafy vegetables—white leaf beets (Beta vulgaris var. cicla), hooker chives (Allium hookeri), basil (Ocimum basilicum), lemon balm (Melissa officinalis), and spearmint (Mentha spicata)—in an indoor greenhouse facility using two different food production platforms: an aquaponic system (AS) with catfish (Silurus asotus) and a conventional hydroponic system (HS). The results showed that the plant growth in AS outperformed that in HS, while the general growth patterns in AS were significantly affected by the different light sources. In addition, the leaf yield of the herbal plants (basil and lemon balm) in AS was 60%–70% more than that in HS. The leaf growth of hooker chives in AS also showed significant improvement (200%) compared with that in HS. It appears that the nutrient sources provided by fish were remarkable, as the average concentration of NO3–N (64 mg/L) was easily achieved in AS. The proposed AS, therefore, would be a promising alternative to the production and supply of supplement organic food, ultimately contributing to promoting sustainable agriculture in a changing climate.
A disulfidptosis-related glucose metabolism and immune response prognostic model revealing the immune microenvironment in lung adenocarcinoma
Authors: Zhang Kai , Li Gang , Wang Qin , Liu Xin , Chen Hong , Li Fuqiang , Li Shuangyan , Song Xinmao , Li Yi
Published: July 2024

Background

Lung adenocarcinoma accounts for the majority of lung cancer cases and impact survival rate of patients severely. Immunotherapy is an effective treatment for lung adenocarcinoma but is restricted by many factors including immune checkpoint expression and the inhibitory immune microenvironment. This study aimed to explore the immune microenvironment in lung adenocarcinoma via disulfidptosis.

Methods

Public datasets of lung adenocarcinoma from the TCGA and GEO was adopted as the training and validation cohort. Based on the differences in the expression of disulfidptosis -related genes, a glucose metabolism and immune response prognostic model was constructed. The prognostic value and clinical relationship of the model were further explored. Immune-related analyses were performed according to CIBERSORT, ssGSEA, TIDE, IPS.

Results

We verified that the model could accurately predict the survival expectancy of lung adenocarcinoma patients. Patients with lung adenocarcinoma and a low-risk score had better survival outcomes according to the model. Moreover, the high-risk group tended to have an immunosuppressive effect, as reflected by the immune cell components, phenotypes and functions. We also found that the clinically relevant immune checkpoint CTLA-4 was significantly higher in low-risk group (P<0.05), indicating that the high-risk group may suffer worse tumor immunotherapy efficacy. Finally, we found that this model has accurate predictive value for the efficacy of immune checkpoint blockade in non-small cell lung cancer (P<0.05).

Conclusion

The prognostic model demonstrated the feasibility of predicting survival and immunotherapy efficacy via disulfidptosis-related genes and will facilitate the development of personalized anticancer therapy.
A single-centre study on abnormal antinuclear antibodies in children caused by intravenous infusion of gamma globulin
Authors: Xu Li , Zhou Juan , Zhang Yu , Wang Yating , Yan Xin , Wang Li , Tang Xuemei , Luo Chong
Published: July 2024

Objective

To clarify the impact of intravenous infusion of gamma globulin (IVIg) on antinuclear antibodies (ANAs) in children.

Methods

A retrospective analysis was performed on the data of children with nonspecific autoantibody-related diseases whose antinuclear antibody (ANA) and autoantibody profiles were detected in our hospital from January to March 2022. A total of 108 patients with a clear history of IVIg infusion within 28 days composed the IVIg group, and 1201 patients without a history of IVIg infusion composed the non-IVIg group.

Results

All patients in the IVIg group had either positive ANAs or positive autoantibodies. Anti-SSA, anti-Ro52 and anti-AMA Mi2 were the top three autoantibodies in the IVIg group. The proportions of patients who were positive for either of these three autoantibodies in the IVIg group were significantly greater than those in the non-IVIg group (all P<0.5). Spearman correlation analysis revealed that the signal intensities of anti-SSA and anti-Ro52 were negatively correlated with the number of days of ANA detection after IVIg infusion (P<0.05). Multiple logistic analyses revealed that a greater total dosage of IVIg, greater IVIg per kilogram of body weight, and fewer ANA detection days after IVIg infusion were independent risk factors for positive anti-SSA and anti-Ro52 results.

Conclusions

It is recommended that if rheumatic diseases are suspected, ANA detection should be carried out beforeIVIg infusion. But for patients who are positive for at least one of these three autoantibodies after IVIg infusion, doctors should first consider adoptive antibodies.
Integrated transcriptome and 16S rDNA analyses reveal that acute heat stress induces intestinal damage in Gymnocypris eckloni
Authors: Duan Yuting , Li Hejiao , Li Junting , Bai Shuhao , Fu Suxing , Zhou Yinhua , Liu Shidong , Li Rundong , Liu Haiping , Zhou Chaowei , Lei Luo
Published: July 2024

Gymnocypris eckloni (G. eckloni), a cold-water economic fish, is widely cultivated in southwestern China. The increase in extreme summer weather conditions owing to global warming can significantly affect their survival and health. The fish intestine and its microbiota are closely associated with fish feeding and growth, nutritional metabolism, and immune defense. However, the mechanisms underlying the changes in the G. eckloni intestine and its microbiota under acute heat stress remain unknown. In this study, we investigated the effects of acute heat stress on the G. eckloni intestine employing histology, plasma biochemical indices, transcriptomics, and 16S rDNA sequencing. Histological analysis showed that acute heat stress induced significant morphological damage to the intestine, with microvilli detachment and mitochondrial abnormalities in the ultrastructure. Biochemical indicators associated with stress (reactive oxygen species and catalase), inflammation (interleukin-1β and tumor necrosis factor-α), and intestinal permeability (diamine oxidase and lipopolysaccharide) were significantly elevated after acute heat stress, indicating an intestinal inflammatory response and disruption of barrier function. Many DEGs were mined by transcriptomic analysis, with tfrc, pfkp, egln1 enriched in the HlF-1 signaling pathway, hsp70, hsp90aa1 and hspa4 enriched in the Antigen processing and presentation pathway, pmm1, pfkfb3 and hk1 enriched in the Fructose and mannose metabolism patyway. The HIF-1 signaling pathway is a crucial regulatory pathway during acute heat stress in the G. eckloni intestine, while significant downregulation of genes associated with adaptive immunity (mica, hla-dpa1, hla-dpb1, and hla-dqb2) suggested impaired immune function. Additionally, the composition of the intestinal microbiota was dominated by Aeromonas, Citrobacter, and Acinetobacter in the control group; but there was a significant decrease in the abundance of Citrobacter and Acinetobacter, and a significantly increased in Shewanella and Hafnia-Obesumbacterium after acute heat stress. Correlation analyses revealed that changes in the abundance of Hafnia-Obesumbacterium, Buttiauxella, and Pseudomonas were closely associated with changes in gene expression associated with stress, inflammation, and immunity. These results comprehensively demonstrate the adaptive mechanisms of the G. eckloni intestine in response to acutely high temperatures and provide a theoretical basis for the future advancement of artificial culture of cold-water fish.
Perceptions of co-teaching as a pedagogical approach to integrate basic and clinical sciences
Authors: Rodríguez-Martín Ivan , Condés Emilia , Sánchez-Gómez Judit , Azpeleta Clara , Tutor Antonio S. , Lesmes Marta , Gal Beatriz
Published: July 2024

Background

Medical curricula must provide students with basic and clinical competencies for critical reasoning and diagnosing. These competencies are better acquired when basic and clinical science are taught in an integrated and collaborative manner. In this study, we investigate whether supportive co-teaching (SCT) is an effective approach to promote integrated and reasoned learning as well as to help medical students applying theoretical concepts to clinical scenarios taught in a team-based learning (TBL) framework.

Methods

We conducted a concurrent mixed methods study. For the qualitative part, we performed a focus group and semi-structured interviews to clinical and basic science teachers and medical students. Using conventional content analysis, themes were identified deductively. For the quantitative part, an analytical and descriptive observational study of the 2019–2020 cohort of first-year undergraduate medical students was conducted (107 students out of 220 completed the survey). For the descriptive study, questions were grouped into 5 categories.

Results

Deductive themes from the analysis include relationship between clinical and basic science teachers, knowledge integration, methodology, teamwork and integrated Medicine and curricular design. Basic science and clinical teachers highlighted their relationship as critical to increase their mutual knowledge. This was supported by the student’s opinion who very much valued their joint feedback. Regarding knowledge integration, both teachers and students found that horizontal and vertical integration enhanced applicability of basic knowledge to future clinical practice. The TBL methodology was very well perceived by both students and teachers and was highly motivating for students even though the need for commitment. Students considered that this program presented a great opportunity and expressed their interest in maintaining it in the future. These results were supported by the quantitative data.

Conclusion

Our work supports the value of co-teaching in basic and clinical sciences within a TBL framework set in real clinical case scenarios. By employing this approach, students can actively apply their theoretical knowledge to clinical practice, enhancing their critical thinking, problem-solving, and clinical reasoning skills. Our findings can inform curriculum design and improved educational practice, leading to enhanced learning experiences for healthcare students and ultimately better patient care.
In vitro extracellular replication of Wolbachia endobacteria
Authors: Behrmann Lara Vanessa , Meier Kirstin , Vollmer Jennifer , Chiedu Chukwuebuka Chibuzo , Schiefer Andrea , Hoerauf Achim , Pfarr Kenneth
Published: July 2024

Obligate intracellular endobacteria of the genus Wolbachia are widespread in arthropods and several filarial nematodes. Control programs for vector-borne diseases (dengue, Zika, malaria) and anti-filarial therapy with antibiotics are based on this important endosymbiont. Investigating Wolbachia, however, is impeded by the need for host cells. In this study, the requirements for Wolbachia wAlbB growth in a host cell-free in vitro culture system were characterized via qPCRs. A cell lysate fraction from Aedes albopictus C6/36 insect cells containing cell membranes and medium with fetal bovine serum were identified as requisite for cell-free replication of Wolbachia. Supplementation with the membrane fraction of insect cell lysate increased extracellular Wolbachia replication by 4.2-fold. Replication rates in the insect cell-free culture were lower compared to Wolbachia grown inside insect cells. However, the endobacteria were able to replicate for up to 12 days and to infect uninfected C6/36 cells. Cell-free Wolbachia treated with the lipid II biosynthesis inhibitor fosfomycin had an enlarged phenotype, seen previously for intracellular Wolbachia in C6/36 cells, indicating that the bacteria were unable to divide. In conclusion, we have developed a cell-free culture system in which Wolbachia replicate for up to 12 days, providing an in vitro tool to elucidate the biology of these endobacteria, e.g., cell division by using compounds that may not enter the C6/36 cells. A better understanding of Wolbachia biology, and in particular host-symbiont interactions, is key to the use of Wolbachia in vector control programs and to future drug development against filarial diseases.
Gut microbiota causally affects drug-induced liver injury via plasma metabolites: a Mendelian randomization study
Authors: Fu Haoshuang , Zhao Shuang , Song Shuying , Xie Qing
Published: July 2024

Background

The gut microbiota and plasma metabolites play important roles in the progression of drug-induced liver injury (DILI). We investigated the causal associations between the gut microbiota, plasma metabolome, and DILI.

Methods

The summary data for gut microbiota (n = 18,340), plasma metabolome (n = 8,299), and DILI (n = 366,838) were obtained from the large genome-wide association studies. A two-sample Mendelian randomization was performed to explore the associations between the gut microbiota, plasma metabolome, and DILI. Additionally, a two-step Mendelian randomization was performed to explore the potential metabolites.

Results

Five taxa were causally associated with DILI, including Oscillospira [odds ratio (OR) = 2.257, 95% confidence interval (CI) = 1.110–4.590], Blautia (OR = 2.311, 95% CI = 1.010–5.288), Roseburia (OR = 2.869, 95% CI = 1.429–5.761), Fusicatenibacter (OR = 1.995, 95% CI = 1.024–3.890), and Prevotella 7 (OR = 1.549, 95% CI = 1.065–2.253). Moreover, 53 metabolites were causally associated with DILI. After mediation analysis, four taxa were found to affect DILI through five mediation metabolites. N6-carbamoylthreonyladenosine mediated the effect of Blautia on DILI. Acetylcarnitine mediated the effect of Fusicatenibacter on DILI. In addition, 4-cholesten-3-one mediated the effect of Prevotella 7 on DILI. Furthermore, 5,6-dihydrothymine levels and the salicylate-to-citrate ratio mediated the effect of Oscillospira on DILI.

Conclusion

We found that the gut microbiota could affect DILI through plasma metabolites, which could serve as potential biomarkers for risk stratification and elucidate underlying mechanisms for further investigation of DILI.
Geriatric nutritional risk index has a prognostic value for recovery outcomes in elderly patients with brain abscess
Authors: Pei Xu , Zhang Yutu , Jiang Dongfeng , Zhang Meng , Fu Junyan , Niu Yang , Tian Mi , Huang Shanshan
Published: July 2024

Background

The Geriatric Nutritional Risk Index (GNRI) is a straightforward and objective tool for nutritional screening in older patients and has been demonstrated to possess prognostic predictive value in several diseases. Nonetheless, there is a lack of research on the nutritional risk associated with brain abscess in the older. This study aimed to evaluate the prevalence of nutritional risk among these patients by GNRI and to investigate its potential prognostic value for clinical outcomes.

Materials and methods

From August 2019 to April 2023, 100 older patients diagnosed with brain abscess were enrolled in this single-center prospective cohort study, which evaluated the prognostic value of the Geriatric Nutritional Risk Index (GNRI) in elderly brain abscess patients. Data collected included demographic, and clinical characteristics at admission and calculated the GNRI, and the Glasgow Outcome Scale (GOS) score 6 months post-discharge. A GOS score of 5 was considered indicative of a good recovery, whereas scores ranging from 1 to 4 were classified as poor recovery.

Results

The results revealed that 48% of older brain abscess patients were at risk of malnutrition according to the GNRI. These patients had significantly higher post-admission C-reactive protein (CRP) levels (p = 0.017), more comorbidities (p < 0.001), and higher age-adjusted Charlson Comorbidity Index (aCCI) scores (p < 0.001) compared to those without nutritional risk. Spearman correlation analysis showed that GNRI scores were negatively correlated with CRP levels, comorbidities, and aCCI scores, and positively correlated with Glasgow Outcome Scale (GOS) scores (Spearman’s ρ = 0.624, p < 0.001). Multivariate logistic regression revealed that lower GNRI values were linked to reduced GOS levels (OR = 0.826, 95% CI: 0.775–0.880). ROC analysis determined a GNRI threshold of 97.50 for predicting poor recovery, with 90.57% sensitivity and 87.23% specificity.

Conclusion

The older brain abscess patients exhibited a high malnutrition risk. GNRI showed an important predictive value for recovery in older patients, which could be helpful in clinical intervention and rehabilitation.
3D bioprinted CRC model brings to light the replication necessity of an oncolytic vaccinia virus encoding FCU1 gene to exert an efficient anti-tumoral activity
Authors: Marquette Christophe A. , Petiot Emma , Spindler Anita , Ebel Caroline , Nzepa Mael , Moreau Baptiste , Erbs Philippe , Balloul Jean-Marc , Quemeneur Eric , Zaupa Cécile
Published: July 2024

The oncolytic virus represents a promising therapeutic strategy involving the targeted replication of viruses to eliminate cancer cells, while preserving healthy ones. Despite ongoing clinical trials, this approach encounters significant challenges. This study delves into the interaction between an oncolytic virus and extracellular matrix mimics (ECM mimics). A three-dimensional colorectal cancer model, enriched with ECM mimics through bioprinting, was subjected to infection by an oncolytic virus derived from the vaccinia virus (oVV). The investigation revealed prolonged expression and sustained oVV production. However, the absence of a significant antitumor effect suggested that the virus’s progression toward non-infected tumoral clusters was hindered by the ECM mimics. Effective elimination of tumoral cells was achieved by introducing an oVV expressing FCU1 (an enzyme converting the prodrug 5-FC into the chemotherapeutic compound 5-FU) alongside 5-FC. Notably, this efficacy was absent when using a non-replicative vaccinia virus expressing FCU1. Our findings underscore then the crucial role of oVV proliferation in a complex ECM mimics. Its proliferation facilitates payload expression and generates a bystander effect to eradicate tumors. Additionally, this study emphasizes the utility of 3D bioprinting for assessing ECM mimics impact on oVV and demonstrates how enhancing oVV capabilities allows overcoming these barriers. This showcases the potential of 3D bioprinting technology in designing purpose-fit models for such investigations.
Immune checkpoint inhibitors targeting PD-1/PD-L1 in the treatment of human lymphomas
Authors: Ribatti Domenico , Cazzato Gerardo , Tamma Roberto , Annese Tiziana , Ingravallo Giuseppe , Specchia Giorgina
Published: July 2024

Non-Hodgkin lymphomas (NHLs) encompass a diverse group of malignancies arising from B cells, T cells, and natural killer (NK) cells at various stages of differentiation. Conversely, classical Hodgkin lymphomas (cHLs) primarily feature Reed-Sternberg cells (RSCs) amid a background of reactive immune cells. Immunomodulatory pathways, notably the PD-1/PD-L1 axis, play pivotal roles in tumor immune evasion across both NHLs and cHLs. Elevated expression of PD-1 and PD-L1 is observed in a spectrum of lymphomas, influencing prognosis and treatment response. Therapeutically, immune checkpoint inhibitors (ICIs) targeting PD-1/PD-L1 have revolutionized lymphoma management, particularly in relapsed/refractory cases. Nivolumab and pembrolizumab, among others, have demonstrated efficacy in various B-cell lymphomas, with promising outcomes in cHL. Combination strategies incorporating ICIs with conventional chemotherapy or targeted agents show enhanced efficacy and are being explored extensively. In this review we discuss the most important features of the tumor microenvironment of NHLs and cHLs, address the therapeutic approaches with ICIs and try to outline future perspectives.
Targeting Notch signaling pathways with natural bioactive compounds: a promising approach against cancer
Authors: Yang Jia , Sun Qihui , Liu Xiaoyun , Yang Yong , Rong Rong , Yan Peiyu , Xie Ying
Published: July 2024

Notch signaling pathway is activated abnormally in solid and hematological tumors, which perform essential functions in cell differentiation, survival, proliferation, and angiogenesis. The activation of Notch signaling and communication among Notch and other oncogenic pathways heighten malignancy aggressiveness. Thus, targeting Notch signaling offers opportunities for improved survival and reduced disease incidence. Already, most attention has been given to its role in the cancer cells. Recent research shows that natural bioactive compounds can change signaling molecules that are linked to or interact with the Notch pathways. This suggests that there may be a link between Notch activation and the growth of tumors. Here, we sum up the natural bioactive compounds that possess inhibitory effects on human cancers by impeding the Notch pathway and preventing Notch crosstalk with other oncogenic pathways, which provoke further study of these natural products to derive rational therapeutic regimens for the treatment of cancer and develop novel anticancer drugs. This review revealed Notch as a highly challenging but promising target in oncology.
Case report: safety and efficacy of lidocaine infusion for the treatment of intractable zoster-associated neuralgia in solid organ transplant recipients
Authors: Zheng Huan , Zheng Bixin
Published: July 2024

Introduction

Solid organ transplant recipients are at high risk for developing severe zoster-associated neuralgia, and the pharmaceutic therapies of pain management for these patients with limited organ function are challenging. Intravenous lidocaine infusion showed positive analgesic effects and is used for the management of neuropathic pain. This case series reports the safety and effectiveness of intravenous lidocaine infusion in the treatment of intractable zoster-associated neuralgia in solid organ transplant recipients.

Case series presentation

Five solid organ transplant recipients suffering from refractory zoster-associated neuralgia (numeric rating scale 8–10, despite using high doses of antiepileptic drugs or combined with opioids) were enrolled. Intravenous lidocaine (5 mg/kg ideal bodyweight) was administered over 1.5 h with the monitoring of vital signs. Pain intensity, patient satisfaction, adverse events, typical liver, and kidney function were evaluated. All subjects reported high satisfaction with their treatment and effective pain relief at the 6-month follow-up. One patient experienced short and mild numbness in the mouth and dizziness after the therapy, but no major adverse reactions were reported.

Conclusion

This case series provides evidence that intravenous lidocaine infusion provided effective pain relief as an analgesic treatment option for transplant patients with intractable zoster-associated neuralgia.
Identifying potential therapeutic targets in lung adenocarcinoma: a multi-omics approach integrating bulk and single-cell RNA sequencing with Mendelian randomization
Authors: Chen Youpeng , Li Enzhong , Chang Zhenglin , Zhang Tingting , Song Zhenfeng , Wu Haojie , Cheng Zhangkai J. , Sun Baoqing
Published: July 2024

Our research aimed to identify new therapeutic targets for Lung adenocarcinoma (LUAD), a major subtype of non-small cell lung cancer known for its low 5-year survival rate of 22%. By employing a comprehensive methodological approach, we analyzed bulk RNA sequencing data from 513 LUAD and 59 non-tumorous tissues, identifying 2,688 differentially expressed genes. Using Mendelian randomization (MR), we identified 74 genes with strong evidence for a causal effect on risk of LUAD. Survival analysis on these genes revealed significant differences in survival rates for 13 of them. Our pathway enrichment analysis highlighted their roles in immune response and cell communication, deepening our understanding. We also utilized single-cell RNA sequencing (scRNA-seq) to uncover cell type-specific gene expression patterns within LUAD, emphasizing the tumor microenvironment’s heterogeneity. Pseudotime analysis further assisted in assessing the heterogeneity of tumor cell populations. Additionally, protein-protein interaction (PPI) network analysis was conducted to evaluate the potential druggability of these identified genes. The culmination of our efforts led to the identification of five genes (tier 1) with the most compelling evidence, including SECISBP2L, PRCD, SMAD9, C2orf91, and HSD17B13, and eight genes (tier 2) with convincing evidence for their potential as therapeutic targets.
Metabolic characterization of the new benzimidazole synthetic opioids - nitazenes
Authors: Jadhav Gajanan R. , Fasinu Pius S.
Published: July 2024

The recent re-emergence and the increasing popularity of nitazenes, a group of new synthetic opioids (NSO) that belong to the benzimidazole chemical class, has raised public health concerns. As a class of potential opioid analgesic agents whose development was discontinued in the 1960s due to their high potential for abuse, very little is known about their metabolism and physiologic disposition. In the current study, three nitazenes–butonitazene, isotonitazene and protonitaze were incubated in human liver microsomes (HLM), human S9 (HS9) fractions and recombinant cytochrome P450 enzymes. All three nitazenes were rapidly metabolized in both HLM and HS9 with over 95% depletion within 60 min. In HLM, butonitazene, isotonitazene and protonitazene had in vitro intrinsic clearance (CLint) (µL/min/mg protein) values of 309, 221 and 216 respectively compared to 150 of verapamil, the positive control. In HS9, CLint values were 217, 139, and 150 for butonitazene, isotonitazene and protonitazene respectively compared to only 35 for testosterone, the control probe substrate. Putative metabolite identified from this study include products of hydroxylation, desethylation, dealkylation, desethylation followed by dealkylation, and desethylation followed by hydroxylation. The metabolic phenotyping showed CYP2D6, CYP2B6 and CYP2C8 and the major hepatic enzymes responsible for the metabolism of nitazenes. Within 30 min of incubation, CYP2D6 depleted butonitazene (99%), isotonitazene (72%) and butonitazene (100%) significantly. The rapid metabolism of nitazenes may be an important factor in accurate and timely detections and quantitation of the unchanged drugs in human matrices following intoxication or in forensic analysis. The involvement of multiple polymorphic CYPs in their metabolism may play important roles in the susceptibility to intoxication and/or addiction, depending on the activity of the metabolites.
Game-theory based truck platoon avoidance modes selection near the highway off-ramp in mixed traffic environment
Authors: Li Yi , Wang Lan , Xuan Zhaoze , Shen Wenzhe
Published: July 2024

Introduction: The rise of autonomous vehicles has brought about a transformative shift in transportation, witnessing the coexistence of human-driven and autonomous vehicles on highways in the United States, Europe, and China. This coexistence poses challenges to traffic operations, particularly in intricate scenarios like highway ramps. The interaction between autonomous truck platoons, displaying heightened maneuverability, and human-driven vehicles has emerged as a critical concern. Consequently, this research aims to propose and investigate three avoidance modes (overall, gap and cross) employed by truck platoons, evaluating their comprehensive impact on human-driven vehicles.

Methods: Multiple scenarios are simulated utilizing the Simulation of Urban Mobility (SUMO) software, collecting data on three distinctive avoidance modes concerning Travel Time (TT) and Time to Collision (TTC). Employing principles of game theory, a comprehensive assessment is undertaken to evaluate the traffic efficiency and safety of each mode. Comparative analyses against a no-avoidance baseline are conducted, offering a holistic evaluation of each mode’s applicability across diverse scenarios.

Results: The findings highlight the commendable performance of gap mode and overall mode in enhancing traffic efficiency, while cross mode excels in fortifying traffic safety. Overall, the gap mode emerges as the optimal choice among the three.

Discussion: This study introduces a game-theoretic approach to managing human-machine mixed traffic flow, establishing a foundational framework for theoretical research in decision-making for emerging mixed traffic environments. It considers safety and efficiency perspectives across different types of traffic entities. The insights gained contribute to the evolving discourse on the integration of autonomous vehicles into existing traffic systems, addressing the intricate challenges posed by the coexistence of various vehicle types on highways.
Research on the suitability and spatial and temporal evolution of innovation environment niche suitability of regional innovation ecosystem under digitalization
Authors: He Zitong , Wang Haijun , Ma Xiaolin , Hu Yuhan , Zhao Huiyan
Published: July 2024

Regional innovation ecosystems (RIEs) are a new paradigm in innovation research, and the development of RIEs under digitization is constrained by the development of innovation environments. The study of ecological niche suitability of regional innovation environment provides the basis for the government to create an innovation environment suitable for the development of a regional innovation ecosystem. This paper conducts an empirical analysis based on panel data from 29 provinces (autonomous regions) in China from 2012 to 2021. The paper analyzes the suitability of the ecological niche of the innovation environment as well as the spatio-temporal evolution pattern of the regional innovation ecosystem under digitalization in China. The results of the study show that: (1) The innovation environment of regional innovation ecosystems under digitization in China has become increasingly suitable during the sample period. (2) From the perspective of evolution in time, the suitability of the ecological niche of the regional innovation environment has always maintained an evolutionary trend of rolling forward in the echelon. (3) From the perspective of spatial evolution, the center line of the curve of the development level of the suitability of the regional innovation environment shows a rightward shifting evolution.
Understanding grain development in the Poaceae family by comparing conserved and distinctive pathways through omics studies in wheat and maize
Authors: Ji Yuanyuan , Hewavithana Thulani , Sharpe Andrew G. , Jin Lingling
Published: July 2024

The Poaceae family, commonly known as the grass family, encompasses a diverse group of crops that play an essential role in providing food, fodder, biofuels, environmental conservation, and cultural value for both human and environmental well-being. Crops in Poaceae family are deeply intertwined with human societies, economies, and ecosystems, making it one of the most significant plant families in the world. As the major reservoirs of essential nutrients, seed grain of these crops has garnered substantial attention from researchers. Understanding the molecular and genetic processes that controls seed formation, development and maturation can provide insights for improving crop yield, nutritional quality, and stress tolerance. The diversity in photosynthetic pathways between C3 and C4 plants introduces intriguing variations in their physiological and biochemical processes, potentially affecting seed development. In this review, we explore recent studies performed with omics technologies, such as genomics, transcriptomics, proteomics and metabolomics that shed light on the mechanisms underlying seed development in wheat and maize, as representatives of C3 and C4 plants respectively, providing insights into their unique adaptations and strategies for reproductive success.
Influencing factors of different metabolic status in hospitalized patients with schizophrenia
Authors: Ding Mubing , Zhang Shaotong , Zhu Zaochen , Cai Renliang , Fang Jin , Zhou Chao , Zhang Xiangrong , Fang Xinyu
Published: July 2024

Objective

The aim of this study was to explore the risk factors for different metabolic status in patients with schizophrenia.

Methods

A total of 968 hospitalized patients with schizophrenia were recruited. Fasting blood glucose (GLU) and lipid profile, including total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG) were measured. Schizophrenia patients were divided into four groups: normal metabolism and weight (NMNW), abnormal metabolism and normal weight (AMNW), normal metabolism and overweight/obesity (NMO), and abnormal metabolism and overweight/obesity (AMO).

Results

Our results showed that NMNW, AMNW, NMO, and AMO accounted for 25.3%, 12.7%, 25.4%, and 36.6%, respectively. There were significant differences in age, disease duration, body mass index (BMI), waist circumference, chronic disease, systolic blood pressure (SBP), diastolic blood pressure (DBP), GLU, TG, TC, HDL-C, and LDL-C among these four groups (all p < 0.05). With the NMNW group as the reference, the disordered multiple classification regression analysis showed that chronic disease was a significant risk factor for AMNW (OR = 5.271, 95% CI = 3.165 to 8.780, p < 0.001) and AMO (OR = 3.245, 95% CI = 2.004 to 5.254, p < 0.001), age was an important protective factor for NMO (OR = 0.968, 95% CI = 0.943 to 0.994, p = 0.015) and AMO (OR = 0.973, 95% CI = 0.948 to 0.999, p < 0.042), waist circumference was a significant risk factor for NMO (OR = 1.218, 95% CI = 1.180 to 1.257, p < 0.001) and AMO (OR = 1.252, 95% CI = 1.212 to 1.291, p < 0.001), and college education was an obvious protective factor for AMO (OR = 0.343, 95% CI = 0.123 to 0.953, p < 0.040) among patients with schizophrenia.

Conclusion

The findings of our study underscored the importance of factors such as age, education level, chronic disease, and waist circumference when exploring the influencing factors and biological mechanisms of obesity-related metabolic problems in schizophrenia patients.
Socioeconomic inequality as a predictor of unmet health needs in the older adult population of Serbia
Authors: Savić Nikola , Radević Svetlana , Jovanović Verica , Ranković Nevena , Lukić Igor , Bogdanović Vasić Slobodanka , Arandjelović Branimirka , Bajić Biljana , Mirković Andrea , Arnaut Aleksandra , Bajić Borko , Vukosavljević Svetlana , Kocić Sanja
Published: July 2024

Objectives

The aim of this paper is to assess the relationship between demographic and socioeconomic predictors and the unmet health needs of the older adult population in Serbia.

Materials and methods

The study is part of the Population Health Survey of Serbia, which was conducted in the period from October to December 2019 by the Institute for Public Health of Serbia “Dr. Milan Jovanović Batut” and the Ministry of Health of the Republic of Serbia. The research was conducted on a representative sample of Serbian residents in the form of a cross-sectional study. For the purposes of this research study, data on senior citizens, aged 65 and older, were used.

Results

Multivariate regression analysis of demographic characteristics that showed statistical significance in the univariate model as a whole explains between 4.2% (Cox & Snell R Square) and 5.9% (Nagelkerke R Square) of the variance of unmet health needs and correctly classifies 66.3% cases. Statistically significant demographic predictors were the region where the respondents live, level of education, and material condition. The results of the research show that the most dominant predictors of the unmet health needs of the older adult population are related to socioeconomic inequalities, financial reasons, and predictors related to the inaccessibility of health care.

Conclusion

The results suggest that individual socioeconomic predictors have a great influence on the emergence of unmet health needs of the older adult population in Serbia. Every third older adult resident did not receive the necessary health care, most often due to financial constraints.
The effect of educational intervention based on the PRECEDE-PROCEED model on self-care behaviors and quality of life of hypertensive patients
Authors: Rakhshani Tayebeh , Tahmasebi Zahra , Ghahremani Leila , Kamyab Amirhossein , Khani Jeihooni Ali
Published: July 2024

Background

To prevent the harmful consequences of hypertension and enhance the quality of life of hypertensive patients, the use of educational models is highly suggested. Therefore, the present study was designed to determine the effect of education based on the PRECEDE-PROCEED on self-care behaviors and the quality of life of hypertensive patients in Kazeroon city, Iran, in 2023.

Methods

A total of 120 hypertensive individuals who were referred to Kazeroon city health centers participated in the current quasi-experimental study. The participants were divided into two experimental and control groups using a random sampling technique (60 participants in each group). The self-care behaviors questionnaire, the quality of life questionnaire, and a questionnaire based on the PRECEDE-PROCEED model were used as the data acquisition techniques. Both groups completed the questionnaires before and 2 months after the intervention. The educational program included a six-session, 50–60 min training program using three different teaching methods (speaking, Q&A, group discussion, and peer training) in health facilities. The data were examined using paired t, independent t, and chi-square statistical tests after being entered into the SPSS 24 statistical program.

Results

Following the intervention, the experimental group showed significantly higher values in quality of life, knowledge, attitude, enabling and reinforcing factors, and self-care behaviors compared to the control group (p < 0.001 for all comparisons). The experimental group also exhibited a significant reduction in systolic blood pressure measures compared to the control group (p < 0.001).

Conclusion

In the present study, education based on the PRECEDE-PROCEED model and focusing on blood pressure self-care behavior in patients with hypertension led to a decrease in their systolic blood pressure measures and improved their quality of life.
Science behind children’s handwashing: action study of 9- to 10-years-old elementary school students in Japan
Authors: Oura Asae , Naito Yukiko , Yako-Suketomo Hiroko , Nakata Kei , Koyama Masayuki , Ohnishi Hirofumi
Published: July 2024

Background

Hand washing instructions for children have been implemented in school education to establish good lifestyle habits. However, repeated hand washing through education from early childhood was common for both teachers and children. If this continues, children might assume they already know how to wash their hands, stop taking handwashing instructions seriously, and become increasingly lax about washing their hands.

Purpose

This study aimed to develop a new handwashing education method for children.

Methods

We applied the adenosine triphosphate (ATP) test to health education on hand washing in elementary schools. This study was conducted as part of a class for elementary school students in October 2023, in Hokkaido, Japan. The subjects were 157 third-grade (9–10 years old) elementary school students. After excluding absent pupils, 147 were included in the analysis.

Results

Both pre- and post-education, ATP values after handwashing were lower than those before handwashing. Following the education, children’s handwashing behavior improved, with an increase in the number of point washed and appropriate timing of handwashing.

Conclusion

The new handwashing education program utilizing the ATP-test succeeded in promoting handwashing behavior among many children. Visualizing handwashing using ATP values was effective in motivating children.
Professional narratives on continued training in the laboral landscape of social education
Authors: Campillo Díaz Margarita , Ayala de la Peña Amalia , Álvarez Muñoz José Santiago , Hernández Prados Ma Ángeles
Published: July 2024

Introduction

Labor consolidation is not a sufficient criterion to abandon the need for the qualification and requalification of professionals, especially in the field of education, which must respond to the uncertainties of society in the light of trends and advances incorporated in pedagogical research.

Methods

The present qualitative study analyzes the training demands of social education professionals from their own perspective, using their own stories in a semi-structured interview format conducted with), key informants from governmental and non-governmental organizations in southeastern Spain, specifically from the autonomous community of the region of Murcia.

Results

According to the results, the most frequent and significant formative limitations are those referred to in the field of social policies, legislative training, administrative processing, specialized work in specific sectors or collectives, and mediation. Similarly, the results reveal fashion themes (mental health, gender) and reiteration of non-exclusive conditioners of social education. These are extrapolable to other areas, such as the increasing bureaucracy and complexity in the proceedings as common places in the reflection of professionals with their own initiative and commitment to their own updating that is associated with a reflective criticism of their own professionalization.

Discussion

The diverse range of responses and subjects, as indicated by the numerous descriptors needed for the categorization and their respective percentages, leads us to conclude that ongoing professional training does not encourage excessive specialization. Instead, it necessitates offering a broad range of training adjusted to the versatility of situations.
Groundwater fluoride prediction modeling using physicochemical parameters in Punjab, India: a machine-learning approach
Authors: Kerketta Anjali , Kapoor Harmanpreet Singh , Sahoo Prafulla Kumar
Published: July 2024

Introduction

Rising fluoride levels in groundwater resources have become a worldwide concern, presenting a significant challenge to the safe utilization of water resources and posing potential risks to human well-being. Elevated fluoride and its vast spatial variability have been documented across different districts of Punjab, India, and it is, therefore, imperative to predict the fluoride levels for efficient groundwater resources planning and management.

Methods

In this study, five different models, Support Vector Machine (SVM), Random Forest (RF), Extreme Gradient Boosting (Xgboost), Extreme Learning Machine (ELM), and Multilayer Perceptron (MLP), are proposed to predict groundwater fluoride using the physicochemical parameters and sampling depth as predictor variables. The performance of these five models was evaluated using the coefficient of determination (R2), mean absolute error (MAE), and root mean square error (RMSE).

Results and discussion

ELM outperformed the remaining four models, thus exhibiting a strong predictive power. The R2, MAE, and RMSE values for ELM at the training and testing stages were 0.85, 0.46, 0.36 and, 0.95, 0.31, and 0.33, respectively, while other models yielded inferior results. Based on the relative importance scores, total dissolved solids (TDS), electrical conductivity (EC), sodium (Na+), chloride (Cl−), and calcium (Ca2+) contributed significantly to model performance. High variability in the target (fluoride) and predictor variables might have led to the poor performance of the models, implying the need for better data pre-processing techniques to improve data quality. Although ELM showed satisfactory results, it can be considered a promising model for predicting groundwater quality.
Multicriteria analysis of sustainable agri-food waste management for an agroecosystem in Finland
Authors: Marttila Miika , Havukainen Jouni , Uusitalo Ville , Linnanen Lassi , Mikkilä Mirja
Published: July 2024

Introduction

The reliance on fossil inputs of agricultural practices has led to maximizing yields and profitability, even at the expense of environmental sustainability. Implementing circular waste management solutions could help align the economic and environmental goals of farmers while reducing their reliance on fossil inputs. The co-application of anaerobic digestion and pyrolysis has been recognized as a potential solution to assist in the transition towards sustainable agriculture.

Objectives

This research assesses different waste management strategies of the major sidestreams generated in agroecosystems in Finland from a climate impact and financial perspective, particularly focusing on the integration of anaerobic digestion and pyrolysis as an emerging solution.

Methods

Six waste management options representing manure application, composting, incineration, anaerobic digestion, pyrolysis, and a co-application process of anaerobic digestion and pyrolysis, are simultaneously assessed with cash flow analysis and life cycle assessment.

Results

The results indicate that co-applying anaerobic digestion and pyrolysis strengthens the performance of a farm through reduced impact on climate, diversified revenue streams, and increased security of supply, even though the direct, short-term financial benefits remain marginal. The capability of co-application of anaerobic digestion and pyrolysis to reduce the climate impacts within the studied system supports the consensus that sustainable and self-sufficient agroecosystems could be supported by this solution in the future.

Conclusion

The adoption of more sustainable agricultural practices requires further expansion of technologies and additional value creation mechanisms, such as stronger employment of carbon markets, to even the economic competition and favor low-carbon operations for practitioners.
Rebuilding strength: surgical intervention and rehabilitation for bilateral spontaneous quadriceps tendon rupture—a case report
Authors: Dong Huaize , Yang Jin , Yu Hao , Zhu Jinsong , Yang Jibin
Published: July 2024

The quadriceps tendon, crucial for body movement, is among the body's strongest tendons. Factors like diabetes or hormone use can weaken it, making even minor trauma potentially causing rupture. Bilateral spontaneous quadriceps tendon rupture, where both tendons tear simultaneously, is rare. Prompt diagnosis and treatment are crucial. We present a case of a 44-year-old woman who experienced bilateral rupture after falling while doing chores. She had immediate pain and limited knee movement. Diagnosis via physical examination and CT/MRI scans confirmed the rupture. Surgical repair followed by rehabilitation led to significant pain reduction and improved function within two months. Overall, her postoperative outcome was satisfactory. This study underscores the importance of clear diagnosis, timely surgery, and thorough rehabilitation for optimal patient recovery from bilateral quadriceps tendon rupture.