bluuebunny/crossref_metadata_2025 huggingface dataset:
Receptor Tyrosine Kinases Amplified in Diffuse-Type Gastric Carcinoma: Potential Targeted Therapies and Novel Downstream Effectors
Authors: Yamaguchi Hideki , Nagamura Yuko , Miyazaki Makoto
Published: August 2022

Gastric cancer (GC) is a major cause of cancer-related death worldwide. Patients with an aggressive subtype of GC, known as diffuse-type gastric carcinoma (DGC), have extremely poor prognoses. DGC is characterized by rapid infiltrative growth, massive desmoplastic stroma, frequent peritoneal metastasis, and high probability of recurrence. These clinical features and progression patterns of DGC substantially differ from those of other GC subtypes, suggesting the existence of specific oncogenic signals. The importance of gene amplification and the resulting aberrant activation of receptor tyrosine kinase (RTK) signaling in the malignant progression of DGC is becoming apparent. Here, we review the characteristics of RTK gene amplification in DGC and its importance in peritoneal metastasis. These insights may potentially lead to new targeted therapeutics.
ENO2 Promotes Colorectal Cancer Metastasis by Interacting with the LncRNA CYTOR and Activating YAP1-Induced EMT
Authors: Lv Chunwei , Yu Hongfei , Wang Keyi , Chen Chaoyi , Tang Jinlong , Han Fengyan , Mai Minglang , Ye Kehong , Lai Maode , Zhang Honghe
Published: August 2022

The glycolytic enzyme enolase 2 (ENO2) is dysregulated in many types of cancer. However, the roles and detailed molecular mechanism of ENO2 in colorectal cancer (CRC) metastasis remain unclear. Here, we performed a comprehensive analysis of ENO2 expression in 184 local CRC samples and samples from the TCGA and GEO databases and found that ENO2 upregulation in CRC samples was negatively associated with prognosis. By knocking down and overexpressing ENO2, we found that ENO2 promoted CRC cell migration and invasion, which is dependent on its interaction with the long noncoding RNA (lncRNA) CYTOR, but did not depend on glycolysis regulation. Furthermore, CYTOR mediated ENO2 binding to large tumor suppressor 1 (LATS1) and competitively inhibited the phosphorylation of Yes-associated protein 1 (YAP1), which ultimately triggered epithelial–mesenchymal transition (EMT). Collectively, these findings highlight the molecular mechanism of the ENO2–CYTOR interaction, and ENO2 could be considered a potential therapeutic target for CRC.
Association between the Use of Quantitative Sensory Testing and Conditioned Pain Modulation and the Prescription of Medication and Interventional Procedures in Children with Chronic Pain Conditions
Authors: Bruneau Alice , Ferland Catherine E. , Pérez-Medina-Carballo Rafael , Somaini Marta , Mohamed Nada , Curatolo Michele , Ouellet Jean A. , Ingelmo Pablo
Published: August 2022

The evidence supporting the use of pharmacological treatments in pediatric chronic pain is limited. Quantitative sensory testing (QST) and conditioned pain modulation evaluation (CPM) provide information on pain phenotype, which may help clinicians to tailor the treatment. This retrospective study aimed to evaluate the association between the use of QST/CPM phenotyping on the selection of the treatment for children with chronic pain conditions. We retrospectively analyzed the medical records of 208 female patients (mean age 15 ± 2 years) enrolled in an outpatient interdisciplinary pediatric complex pain center. Pain phenotype information (QST/CPM) of 106 patients was available to the prescribing physician. The records of 102 age- and sex-matched patients without QST/CPM were used as controls. The primary endpoint was the proportion of medications and interventions prescribed. The secondary endpoint was the duration of treatment. The QST/CPM group received less opioids (7% vs. 28%, respectively, p < 0.001), less anticonvulsants (6% vs. 25%, p < 0.001), and less interventional treatments (29% vs. 44%, p = 0.03) than controls. Patients with an optimal CPM result tended to be prescribed fewer antidepressants (2% vs. 18%, p = 0.01), and patients with signs of allodynia and/or temporal summation tended to be prescribed fewer NSAIDs (57% vs. 78%, p = 0.04). There was no difference in the duration of the treatments between the groups. QST/CPM testing appears to provide more targeted therapeutic options resulting in the overall drop in polypharmacy and reduced use of interventional treatments while remaining at least as effective as the standard of care.
The Effect of Single Curing Agents on the Curing Reactions of the HTPB-based Binder System
Authors: Ma Hui , Liu Yucun , Chai Tao , Yu Yanwu , Guo Jiahu , Zhong Lunchao , Zhang Qin
Published: August 2022

As the hydroxyl-terminated polybutadiene (HTPB)-based binder system is widely applied in many industries, the curing process plays an important role in the final properties of the resulting product containing such a binder system. This study used a viscometer to measure viscosity buildup in the curing process of the binder system with various curing agents under isothermal conditions. Key parameters such as rheological reaction rate constant (kƞ) and pot life of different were measured and calculated. The rheological reaction rate constants of the HTPB-based binder systems included 0.0423 min−1 (MDI), 0.0049 min−1 (HDI-trimer) and 0.0014 min−1 (HMDI). The pot lives of the HTPB-MDI, HTPB-TDI, and HTPB-HDI-trimer were 0.6 h, 3.6 h and 8.1 h, respectively. One interesting finding is that HTPB-HDI-trimer binder, which had the long pot life, exhibited an accelerated trend in the viscosity buildup in the late phase. This feature is of great significance to improving the final properties of the products generated in propellant manufacturing and other fields. The cause of this phenomenon and the curing process of HTPB-HDI-trimer binder system were analyzed and discussed in the present study.
Clinical Outcomes and Severity of Acute Respiratory Distress Syndrome in 1154 COVID-19 Patients: An Experience Multicenter Retrospective Cohort Study
Authors: Al Mutair Abbas , Alhumaid Saad , Layqah Laila , Shamou Jinan , Ahmed Gasmelseed Y. , Chagla Hiba , Alsalman Khulud , Alnasser Fadhah Mohammed , Thoyaja Koritala , Alhuqbani Waad N. , Alghadeer Mohammed , Al Mohaini Mohammed , Almahmoud Sana , Al-Tawfiq Jaffar A. , Muhammad Javed , Al-Jamea Lamiaa H. , Woodman Alexander , Alsaleh Ahmed , Alsedrah Abdulaziz M. , Alharbi Hanan F. , Saha Chandni , Rabaan Ali A.
Published: August 2022

Background: Acute Respiratory Distress Syndrome (ARDS) is caused by non-cardiogenic pulmonary edema and occurs in critically ill patients. It is one of the fatal complications observed among severe COVID-19 cases managed in intensive care units (ICU). Supportive lung-protective ventilation and prone positioning remain the mainstay interventions. Purpose: We describe the severity of ARDS, clinical outcomes, and management of ICU patients with laboratory-confirmed COVID-19 infection in multiple Saudi hospitals. Methods: A multicenter retrospective cohort study was conducted of critically ill patients who were admitted to the ICU with COVID-19 and developed ARDS. Results: During our study, 1154 patients experienced ARDS: 591 (51.2%) with severe, 415 (36.0%) with moderate, and 148 (12.8%) with mild ARDS. The mean sequential organ failure assessment (SOFA) score was significantly higher in severe ARDS with COVID-19 (6 ± 5, p = 0.006). Kaplan–Meier survival analysis showed COVID-19 patients with mild ARDS had a significantly higher survival rate compared to COVID-19 patients who experienced severe ARDS (p = 0.023). Conclusion: ARDS is a challenging condition complicating COVID-19 infection. It carries significant morbidity and results in elevated mortality. ARDS requires protective mechanical ventilation and other critical care supportive measures. The severity of ARDS is associated significantly with the rate of death among the patients.
Validity of the Isometric Contraction Test of the Masticatory Muscles for Diagnosis of Muscular Temporomandibular Disorders
Authors: Iglesias-Peón Marcos , Mesa-Jiménez Juan , Fernández-de-las-Peñas César , Rojas-García Jorge , Rodrigues-de-Souza Daiana Priscila , Alburquerque-Sendín Francisco
Published: August 2022

In recent years, the Diagnostic Criteria for Temporomandibular Pain Disorders (DC/TMD) has been questioned, mainly because of the dependence on the muscular pressure needed to be applied during the clinical examination. Therefore, it is necessary to establish improvements in diagnostic strategies for DC/TMD of axis I. The aim of this study was to determine the validity of the Isometric Contraction Test of the masticatory muscles (ICTest) to diagnose DC/TMD of axis I. Forty (n = 40) patients with muscular TMD (myalgia in any of its subtypes), as well as forty age and sex matched controls, participated. They were diagnosed according to DC/TMD of axis I and performed the ICTest in a single session. Sensitivity, specificity, positive (PPV) and negative predictive values (NPV), and positive (LR+) and negative likelihood ratios (LR−) of the ICTest compared with the DC/TMD of axis I were calculated. The ICTest showed a specificity of 95% for the diagnosis of myalgia, and between 94.9% and 96.8% for all subtypes in relation to the DC/TMD of axis I. For sensitivity, lower values were obtained, that is, 90.0% for myalgia, and losing sensitivity depending on the type of myalgia. The LR+ was over 10 for all diagnoses, with the exception of myofascial pain with referral, which was lower. When addressing the LR−, the myofascial diagnosis was the only one below 0.2. According to the results, the ICTest could be considered a valid procedure to diagnose subjects with muscular TMD in a clinical setting.
Segmentation Method of Cerebral Aneurysms Based on Entropy Selection Strategy
Authors: Li Tingting , An Xingwei , Di Yang , He Jiaqian , Liu Shuang , Ming Dong
Published: August 2022

The segmentation of cerebral aneurysms is a challenging task because of their similar imaging features to blood vessels and the great imbalance between the foreground and background. However, the existing 2D segmentation methods do not make full use of 3D information and ignore the influence of global features. In this study, we propose an automatic solution for the segmentation of cerebral aneurysms. The proposed method relies on the 2D U-Net as the backbone and adds a Transformer block to capture remote information. Additionally, through the new entropy selection strategy, the network pays more attention to the indistinguishable blood vessels and aneurysms, so as to reduce the influence of class imbalance. In order to introduce global features, three continuous patches are taken as inputs, and a segmentation map corresponding to the central patch is generated. In the inference phase, using the proposed recombination strategy, the segmentation map was generated, and we verified the proposed method on the CADA dataset. We achieved a Dice coefficient (DSC) of 0.944, an IOU score of 0.941, recall of 0.946, an F2 score of 0.942, a mAP of 0.896 and a Hausdorff distance of 3.12 mm.
Determinants of Sustainable Growth of SMEs in Developing Countries: The Case of Ethiopia
Authors: Abdissa Gemechu , Ayalew Abebe , Dunay Anna , Bálint Illés Csaba
Published: August 2022

Small and medium-sized businesses (SMEs) played a significant role in domestic employment and inclusive GDP in developing countries such as Ethiopia. Accordingly, the purpose of this study is to investigate the determinants of sustainable growth of SMEs in developing countries, notably in Ethiopia. To achieve the objectives of this study, both descriptive and explanatory research designs were used. In addition to this, primary data was collected from SMEs engaged in service, manufacturing, trade, construction, and urban agriculture. The sampling techniques used for this study is the stratified simple random sampling technique in which 194 employees of SMEs were drawn as a sample size for this study. Moreover, multiple linear regression models were used with the Statistical Package for the Social Sciences (SPSS) version 23 software as the data analysis tool. According to the study’s findings, crucial issues such as the coronavirus and political uncertainty in Ethiopia, which have allowed for widespread corruption, are now harming the growth of SMEs. According to the findings, political instability, corruption, and COVID-19 are now having a substantial effect on the growth of SMEs in Ethiopia. These concerns have serious consequences for the long-term sustainability of Ethiopian SMEs. Hence, the study strongly advocates for sequential policy reform in the region, as well as a review of current policies aimed at ensuring effective corruption control in the region and bringing political stability to the region, particularly in Ambo town, Ethiopia.
Reshaping Sustainable University Education in Post-Pandemic World: Lessons Learned from an Empirical Study
Authors: Munir Hussan
Published: August 2022

The outbreak of COVID-19 has affected people all around the world. Governments had no choice but to put people in self-isolation to stop the spread of the virus. As a result, all companies and educational institutions switched to working or studying from home. The purpose of the study is to investigate the impact of COVID-19 on student teaching and learning in the context of Malmö university. Furthermore, the study proposes recommendations for sustainable post-pandemic education at Malmö University. The study includes ten semi-structured interviews with students followed by a workshop with ten senior lecturers teaching bachelor’s and master’s courses. The study uses snowball sampling to select students for the interviews and senior lecturers for the workshop. A qualitative data analysis technique, thematic analysis, is used for data analysis on the data collected from interviews with students and the workshop with senior lecturers. The results from the study suggested that online education leads to several benefits for students, such as better time management, higher lecture attendance, flexibility, and discipline in their studies. However, the shift to online education has caused a communication deterioration between students and teachers. Less social interaction with other students leads to depression, anxiety, and stress. The recommendations for post-pandemic education include the unified selection of digital learning tools across courses, a designated budget for digital learning tools, training support, and hybrid learning methods. In conclusion, the study proposes blended and hybrid learning to improve higher education at the university, requiring digital tools to minimize students’ communication barriers.
Research on Fingerprint and Hyperbolic Fusion Positioning Algorithm Based on 5G Technology
Authors: Geng Zhiqiang , Yang Jie , Guo Zhiqiang , Cao Hui , Leonidas Lilian
Published: August 2022

With the development of the Internet of Things technology, higher requirements are put forward for the positioning accuracy of objects. This paper presents an indoor fusion positioning algorithm based on the 5th Generation Mobile Communication Technology (5G), which effectively solves two problems. The first is that fingerprint positioning is susceptible to environmental changes and results in inaccurate fingerprint matching. The second is the problem of the hyperbolic positioning algorithm based on the line-of-sight fluctuating too much in complex indoor environments. This paper uses a 5G flexible subcarrier interval of Orthogonal Frequency Division Multiplexing (OFDM) to significantly reduce the time delay error of Time Of Arrival (TOA), and an improved genetic algorithm and the weighted hyperbolic algorithm are used to estimate the optimal position coordinates. In the offline database establishment stage of fingerprint positioning, the Channel State Information-reference signal (CSI-RS) of multiple-beam sets provides high-dimensional information for subsequent training and prediction. The online stage cooperates with the improved residual network model to make predictions. Finally, the positioning information and the error distribution function generated by the two positioning processes are simultaneously used as the input of the Kalman filter to obtain the precise position coordinates. The simulation results show that in complex indoor scenes where line-of-sight propagation and non-line-of-sight propagation paths are mixed, the accuracy of this method can reach below 0.82 m. Thus, the positioning accuracy is significantly improved compared with other methods, which can meet most indoor scene positioning needs.
Effect of Temperature Gradient on Chloride Ion Diffusion in Nuclear Reactor Containment Building Concrete
Authors: An Boohyun , Cho Pyungyeon , Shittu Remilekun A. , Kim Tae-Yeon , Rostron Paul , AlFantazi Akram , Yi Yongsun
Published: August 2022

Chloride diffusion through concrete is influenced by harsh environmental conditions such as high ambient temperature and relative humidity. This paper examined the influence of temperature gradient on chloride diffusion in concrete under high ambient temperature conditions. Chloride diffusion tests using cylindrical concrete samples were performed in constant temperature and temperature gradient conditions. In a temperature gradient condition, a much higher chloride concentration was measured than at constant temperatures, which could not be explained only by the mass diffusion driven by the concentration gradient. A new analytical model of chloride diffusion with the mass diffusion term including the temperature effect and the thermo-diffusion term including the temperature gradient effect was applied to the results, which showed that the thermo-diffusion contribution was significant. Using the analytical model with the mass diffusion (DCl) and thermo-diffusion (DT) coefficients, the service life of reactor containment buildings (RCBs) in nuclear power plants (NPPs) in the Middle Eastern and North African (MENA) region was estimated. The results showed that the service life of the RCBs could be reduced by the temperature gradient, indicating the possible application of the proposed analytical model.
Vom Berg and Hahn–Eyring Drilling Fluid Rheological Models
Authors: Wiśniowski Rafał , Skrzypaszek Krzysztof , Toczek Przemysław
Published: August 2022

This article presents rheological models of fluids used in the drilling practice. It discusses the principles of determining drilling fluid rheological parameters based on data acquired from measurements by means of viscometers used in the drilling practice. The authors propose the application of the three-parameter Vom Berg and Hahn–Eyring models not used in the drilling industry so far. Necessary relationships have been developed for these models, which enable the determination of rheological parameters. In order to account for the influence of different flow conditions on the value of drilling fluid rheological parameters, the approach proposing the determination of rheological parameters of a given three-parameter model separately for low shear rates and high shear rates has been suggested. A practical application of the methodology proposed in this paper for determining the rheological parameters of the three-parameter Vom Berg and Hahn–Eyring models is presented using real drilling fluids as an example. Using the author’s methodology for determining the optimum rheological model, called Rheosolution, described earlier in the paper “Selection of Suitable Rheological Model for Drilling Fluid Using Applied Numerical Methods”, published in Energies 2020, 13, 3192, and laboratory tests performed for this work (for cement slurries according to API standards), a strong correlation of the Vom Berg model and, in particular, the Hahn–Eyring model for such drilling fluids was demonstrated.
Numerical Assessments of Flow and Advective Transport Uncertainty for Performance Measures of Radioactive Waste Geological Disposal in Fractured Rocks
Authors: Yu Yun-Chen , Shen Yu-Hsiang , Lee Tsai-Ping , Ni Chuen-Fa , Lee I-Hsien
Published: August 2022

Groundwater flow and transport are crucial for performance and safety assessment in the radioactive waste geological disposal. This study presents the groundwater flow and advective transport simulations for assessing the performance of a reference repository placed in fractured rocks. The study involves the concept of radionuclides migrating into the mobile water in fractures surrounding the deposition hole and calculates two specific quantitative indicators in the field of radioactive waste geological disposal. The indicators equivalent flow rate (Qeq) and flow-related transport resistance (F) are used to express the groundwater flow and transport resistance in the host rock. Based on the hydrogeological conceptual model, the study employs DarcyTools to model the groundwater flow and advective transport of a base case. This study then conducts sensitivity analyses by varying the hydraulic conductivity of the key hydrogeological unit and the excavation damage zone. The uncertainty analysis employs multiple discrete fracture network (DFN) realizations to quantify the influences of DFNs on the flow and advective transport. Results show that the hydraulic conductivity of host rock dominates the flow and advective transport in the model domain, and the highest Qeq is 1.91 × 10−4 m3/year, and the lowest F is 7.77 × 105 year/m. Results also indicate that simulations of the hydraulic conductivity variations of hydrogeological units are more critical than those obtained from the variations of DFN realizations (i.e., the uncertainty analysis). The solutions could be useful for site investigations to modify the hydrogeological conceptual model in the study.
Thermal Stress Characteristics of Dissimilar Joints Joining Ti-64 and CCM via Linear Friction Welding
Authors: Glaspell Aspen , Peña José Angel Diosdado-De la , Ryu Jae Joong , Choo Kyosung
Published: August 2022

Linear Friction Welding (LFW) is a unique welding process which enables dissimilar materials to be welded without filler material. In this study, bi-metallic Ti-64-CCM joints were manufactured utilizing LFW and subsequently analyzed. A coupled thermal-deformation model was created to accurately model both thermal and deformation profiles along the weld interface. Ti-64 and CCM stock were machined in accordance with ASTM standards for tensile testing. Temperature distribution of the two material surfaces were measured using an IR camera. Tensile testing was then performed to analyze weldability and bonding strength. A transient decoupled thermomechanical simulation model was generated to simulate the LFW process. The simulation model produced results within 5% error of both experimental results. The tensile testing results indicate that successful bonding of Ti-64 to CCM is possible for an oscillation amplitude of 2 mm under large forging pressure. The results also show that parameter optimization is essential for successful LFW, as the decrease in percent error from test set 1 to test set 4 was 62.5%. From the tensile testing and thermal data collected, it was concluded that bonding of Ti-64 to CCM via LFW is possible but further research into the necessary process parameters is needed.
Flexible Interconnected Distribution Network with Embedded DC System and Its Dynamic Reconfiguration
Authors: Cai Huan , Yuan Xufeng , Xiong Wei , Zheng Huajun , Xu Yutao , Cai Yongxiang , Zhong Jiumu
Published: August 2022

Flexible interconnection transformation of a distribution network using all-control power electronics technology will help to overcome the technical bottleneck of distributed generation (DG) penetration and high-quality power supply in the traditional distribution network. However, the flexible interconnection form that uses back-to-back power electronic converters to replace tie switches has disadvantages such as high cost and large footprint, so its mass promotion and application are bound to be limited. Therefore, a new topology of flexible interconnected distribution network with embedded DC system (FDN+EDC) with a better economy is proposed. Firstly, the topology of FDN+EDC is introduced to improve the controllability level of the distribution network under high percentage DG penetration, and is compared with the conventional flexible interconnection method. Secondly, in order to realize the coordinated optimal economic operation of source, network and load in the FDN+EDC, a dynamic reconfiguration model of switches status and continuous adjustment of flexible interconnection device (FID) are proposed. Then, to achieve a fast and globally optimal solution of the model, a hierarchical coordinated optimization strategy is designed based on an improved particle swarm algorithm for the optimization of discrete and continuous variables. Finally, the validity of the algorithm is verified, and an 88-node FDN+EDC is constructed to verify the advantages of the proposed embedded DC flexible interconnection topology and realize the joint optimal dispatch of network-source-load.
Automatic Remote Sensing Identification of Co-Seismic Landslides Using Deep Learning Methods
Authors: Pang Dongdong , Liu Gang , He Jing , Li Weile , Fu Rao
Published: August 2022

Rapid and accurate extraction of landslide areas triggered by earthquakes has far-reaching significance for geological disaster risk assessment and emergency rescue. At present, visual interpretation and field survey are still the most-commonly used methods for landslide identification, but these methods are often time-consuming and costly. For this reason, this paper tackles the problem of co-seismic landslide identification and the fact that there is little sample information in existing studies on landslide. A landslide sample dataset with 4000 tags was produced. With the YOLOv3 algorithm as the core, a convolutional neural network model with landslide characteristics was established to automatically recognize co-seismic landslides in satellite remote sensing images. By comparing it with the graphical interpretation results of remote sensing images, we found that the remote sensing for landslide recognition model constructed in this paper demonstrated high recognition accuracy and fast speed. The F1 value was 0.93, indicating that the constructed model was stable. The research results can provide reference for emergency rescue and disaster investigation of the same co-seismic landslide disaster.
Correction: Roberts et al. Effects of Elevated Atmospheric CO2 Concentration on Insect Herbivory and Nutrient Fluxes in a Mature Temperate Forest. Forests 2022, 13, 998
Authors: Roberts Aradhana J. , Crowley Liam M. , Sadler Jon P. , Nguyen Tien T. T. , Gardner Anna M. , Hayward Scott A. L. , Metcalfe Daniel B.
Published: August 2022

In the original publication [...]
Freeze-Dried Tuna Pepsin Powder Stabilized by Some Cryoprotectants: In Vitro Simulated Gastric Digestion toward Different Proteins and Its Storage Stability
Authors: Patil Umesh , Nikoo Mehdi , Zhang Bin , Benjakul Soottawat
Published: August 2022

The impact of maltodextrin (10%) in combination with trehalose or glycerol at different levels (2.5% and 5%) and their mixture on the stability of freeze-dried pepsin from skipjack tuna stomach was studied. Addition of 5% trehalose and 10% maltodextrin yielded the powder (TPP-T5) with highest relative pepsin activity (p < 0.05). TPP-T5 had different shapes and sizes, with mean particle size of 65.42 ± 57.60 μm, poly-dispersity index of 0.474, and zeta potential of −19.95. It had bulk density of 0.53 kg m−3 and possessed fair flowability. The wetting time for TPP-T5 was 16.36 ± 0.73 min, and solubility was 93.58%. TPP-T5 stored at room temperature under different relative humidities could maintain proteolytic activity up to 4 weeks. Commercial porcine pepsin (CP) and crude tuna pepsinogen had molecular weights of 35.2 and 43.3 kDa, respectively, when analyzed using gel filtration (Sephadex G-50) and SDS-PAGE. Tuna pepsin had comparable hydrolysis toward threadfin bream muscle protein, whey protein isolate, and kidney bean protein isolate to commercial pepsin, especially at a higher level (15 units/g protein). Digested proteins contained peptides with varying molecular weights as determined by MALDI-TOF. Therefore, pepsin from skipjack tuna stomach could replace commercial porcine pepsin and was beneficial supplement for patients with maldigestion, particularly the elderly.
The Antimicrobial and Antibiofilm Activity of Oregano Essential Oil against Enterococcus faecalis and Its Application in Chicken Breast
Authors: Zhan Xiangjun , Tan Yingzhu , Lv Yingmei , Fang Jianing , Zhou Yuanjian , Gao Xing , Zhu Huimin , Shi Chao
Published: August 2022

Oregano essential oil (OEO) possesses anti-inflammatory, antioxidant, and cancer-suppressive properties. Enterococcus faecalis is a foodborne opportunistic pathogen that can be found in nature and the food processing industry. The goal of this investigation was to explore the antimicrobial action and mechanism of OEO against E. faecalis, inactivation action of OEO on E. faecalis in mature biofilms, and its application in chicken breast. The minimum inhibitory concentration (MIC) of OEO against E. faecalis strains (ATCC 29212 and nine isolates) ranged from 0.25 to 0.50 μL/mL. OEO therapy reduced intracellular adenosine triphosphate (ATP) levels, caused cell membrane hyperpolarization, increased the intracellular reactive oxygen species (ROS), and elevated extracellular malondialdehyde (MDA) concentrations. Furthermore, OEO treatment diminished cell membrane integrity and caused morphological alterations in the cells. In biofilms on stainless-steel, OEO showed effective inactivation activity against E. faecalis. OEO reduced the number of viable cells, cell viability and exopolysaccharides in the biofilm, as well as destroying its structure. Application of OEO on chicken breast results in a considerable reduction in E. faecalis counts and pH values, in comparison to control samples. These findings suggest that OEO could be utilized as a natural antibacterial preservative and could effectively control E. faecalis in food manufacturing.
Volatile Compounds Analysis and Biomarkers Identification of Four Native Apricot (Prunus armeniaca L.) Cultivars Grown in Xinjiang Region of China
Authors: Zhao Cai , Sun Jinkui , Pu Xilei , Shi Xuewei , Cheng Weidong , Wang Bin
Published: August 2022

Flavor (odor and taste) have a significant role in the consumer’s acceptance, and volatile compounds are responsible for the odor of apricots. In the present work, headspace solid-phase microextraction with gas chromatography coupled to tandem mass spectrometry (HS-SPME-GC-MS/MS) together with multivariate analysis, i.e., partial least square discrimination analysis (PLS-DA), were applied to construct the volatile fingerprints and biomarkers of apricots in Xinjiang, China. As a result, a total of 63 volatile substances were identified in the fruits of four apricot cultivars, seven of which were considered to serve as volatile biomarkers, which are damascenone for Dabaiyou apricots; acetophenone, myrcenol and 7-hexadecenal for Luopuhongdaike apricots; 2,4-dimethyl-cyclohexanol for You apricots; eucalyptol and salicylaldehyde for Xiaobai apricots. Moreover, Xiaobai apricots were richer in soluble sugars, organic acids and total phenolic and total flavonoid content than the other three apricot varieties. This work helps to characterize the volatile profiles and biomarkers of different apricot cultivars while providing theoretical guidance for developing apricot-flavored foods in practical production.
Impact of Location, Gender and Previous Experience on User Evaluation of Augmented Reality in Cultural Heritage: The Mjällby Crucifix Case Study
Authors: Garro Valeria , Sundstedt Veronica , Sandahl Christoffer
Published: August 2022

In recent decades, a growing number of museums have adopted digital media, both as an enhancement of exhibitions of real artifacts and as an alternative to traditional display methods. The digital acquisition of artifacts generates accurate 3D replicas that can be displayed via different digital media. With an increase in immersive technologies in the cultural heritage (CH) domain, it is common to see digital artifacts presented in Virtual Reality (VR) and Augmented Reality (AR). This paper presents two user studies conducted in different locations evaluating the use of an AR application in the portrayal of the Mjällby Crucifix artifact. This paper presents the overall results from both user studies evaluating and discussing the AR application on a number of different aspects on a 7-point Likert scale: (1) understanding the artifact, (2) ease of use, (3) object feeling part of reality, (4) perceived visual quality of the object, (5) overall satisfaction experience, and (6) willingness to download the AR application. The results have been compared between genders, age groups, and previous experience with AR. Potential benefits and disadvantages of AR experiences in the context of a museum exhibition were also gathered in free text from the visitors.
Establishment of an Efficient In Vitro Propagation Method for a Sustainable Supply of Plectranthus amboinicus (Lour.) and Genetic Homogeneity Using Flow Cytometry and SPAR Markers
Authors: Faisal Mohammad , Alatar Abdulrahman A.
Published: August 2022

Plectranthus amboinicus (Lour.) Spreng is a medicinally important aromatic perennial herb used for the treatment of skin diseases, constipation, asthma, flu, fever, cough, and headache as well as a flavoring ingredient in traditional drinks, food, and meat stuffing. In this study, a high-performance in vitro propagation system of P. amboinicus through direct shoot organogenesis was developed using axillary node explants cultured on MS (Murashige and Skoog) medium augmented with 0.5, 2.5, 5.0, 7.5, and 10.0 µM of 6-benzyladenine (BA) or kinetin (Kin), alone or with 0.1, 0.5, 2.5, and 5.0 µM of indole-3-acetic acid (IAA) or α-naphthalene acetic acid (NAA). To optimize the regeneration potential of node explants, the effects of basal media strength and pH were also investigated. After 8 weeks of culture, explants cultured in full strength MS basal medium (pH 5.7) with 5.0 µM BA and 2.5 µM NAA exhibited the highest percentage (97.1%) of regeneration and the maximum number (19.3) of shoots per explant. Individual elongated shoots were rooted on half strength MS basal medium containing 0.25 µM indole 3-butyric acid (IBA) after 4 weeks of culture, producing 5.3 roots/shootlets with a root induction frequency of 93.7%. First time genetic stability of in vitro raised P. amboinicus plants was determined using SPAR markers, such as DAMD and ISSR, as well as flow cytometric tests, assuring the availability of authenticated raw materials for commercial production of the plant and its bioactive components.
Optimizing Phytochemical and Physiological Characteristics of Balangu (Lallemantia iberica) by Foliar Application of Chitosan Nanoparticles and Myco-Root Inoculation under Water Supply Restrictions
Authors: Javanmard Abdollah , Ashrafi Mahsa , Morshedloo Mohammad Reza , Machiani Mostafa Amani , Rasouli Farzad , Maggi Filippo
Published: August 2022

Balangu is a medicinal plant used in the Iranian traditional medicine to treat nervous, hepatic and renal diseases. To determine the effects of Myco-Root biofertilizer and chitosan nanoparticles (Cs-NPs) on the physiological and biochemical properties of balangu (Lallemantia iberica (M.Bieb.) Fisch. & C.A.Mey.) under different irrigation levels, an experiment was laid out as a factorial based on completely randomized design (CRD) with twelve treatments and three replications. The first factor was represented by different irrigation regimes, including no water deficit (90% FC), mild water deficit (60% FC) and severe water deficit (30% FC); the second factor included control (no Myco-Root and Cs-NPs), inoculation with Myco-Root biofertilizer, foliar application of chitosan nanoparticles (Cs-NPs) and co-application of Cs-NPs along with Myco-Root. The results showed that the highest fresh and dry weight, chlorophyll and carotenoid content, chlorophyll index (SPAD) and fluorescence indices were obtained in 90% FC treated with Cs-NPs+ Myco-Root. In addition, the maximum activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POX) was achieved in 60% FC with application of Cs-NPs+ Myco-Root. Moreover, the maximum essential oil content (1.43%) and yield (0.25 g pot−1) were recorded in 60% FC following the application of Cs-NPs+ Myco-Root. Chemical analysis of essential oil showed that germacrene D (31.22–39.77%), (E)-caryophyllene (16.28–19.82%), bicyclogermacrene (7.1–9.22%) and caryophyllene oxide (3.85–6.96%) were the major volatile constituents of balangu. Interestingly, the maximum contents of germacrene D and (E)-caryophyllene were recorded in 60% FC after the application of Cs-NPs+ Myco-Root. Overall, it can be concluded that co-application of Cs-NPs+ Myco-Root could be a sustainable and eco-friendly strategy for improving the essential oil quantity and quality, as well as physiological characteristics, of balangu under water deficit conditions.
Effects of Shade Nets on Microclimatic Conditions, Growth, Fruit Yield, and Quality of Eggplant (Solanum melongena L.): A Case Study in Carnarvon, Western Australia
Authors: Nguyen Giao N. , Lantzke Neil , van Burgel Andrew
Published: August 2022

Carnarvon has a hot, semi-arid climate with high temperatures and solar radiation during spring–summer, which damages crops and limits the production season for the local vegetable industry. Protective cultivation is one of the promising approaches to mitigate these adverse weather conditions and avoid the resulting damage to vegetable crops. This study, which is part of the protected cropping research program for vegetable crops in Western Australia, was conducted to understand how the shade nets of a protective net house modify the microenvironment affecting the growth, physiology, and fruit yield of eggplants, a model vegetable crop. The eggplant crop was grown under four light regimes, i.e., three shade factors (11%, 21%, 30%) and the open field. There were three replicated blocks under each light regime and four eggplant varieties that were randomized within the replicated blocks. Other experimental conditions, e.g., fertilising, irrigation, pest, and disease management and other cultural practices were identical across light regimes. The results showed that shade nets created different microenvironments inside the net house, with a large variation in the light intensity, affecting photosynthetic-related traits. Eggplants grew taller and bushier and gave higher fruit yield under shade compared to the open field. Overall, our data suggest that the 21% shade net appeared to be the most suitable for growing eggplants during the autumn to early spring period in Carnarvon. The future perspective of protected cropping technology for vegetable crop production in Carnarvon is also discussed.
Inequity in Access and Delivery of Virtual Care Interventions: A Scoping Review
Authors: Mistry Sabuj Kanti , Shaw Miranda , Raffan Freya , Johnson George , Perren Katelyn , Shoko Saito , Harris-Roxas Ben , Haigh Fiona
Published: August 2022

The objectives of this review were to map and summarize the existing evidence from a global perspective about inequity in access and delivery of virtual care interventions and to identify strategies that may be adopted by virtual care services to address these inequities. We searched MEDLINE, EMBASE, and CINAHL using both medical subject headings (MeSH) and free-text keywords for empirical studies exploring inequity in ambulatory services offered virtually. Forty-one studies were included, most of them cross-sectional in design. Included studies were extracted using a customized extraction tool, and descriptive analysis was performed. The review identified widespread differences in accessing and using virtual care interventions among cultural and ethnic minorities, older people, socioeconomically disadvantaged groups, people with limited digital and/or health literacy, and those with limited access to digital devices and good connectivity. Potential solutions addressing these barriers identified in the review included having digitally literate caregivers present during virtual care appointments, conducting virtual care appointments in culturally sensitive manner, and having a focus on enhancing patients’ digital literacy. We identified evidence-based practices for virtual care interventions to ensure equity in access and delivery for their virtual care patients.
Effects of an Online Supervised Exercise Training in Children with Obesity during the COVID-19 Pandemic
Authors: Vandoni Matteo , Carnevale Pellino Vittoria , Gatti Alessandro , Lucini Daniela , Mannarino Savina , Larizza Cristiana , Rossi Virginia , Tranfaglia Valeria , Pirazzi Agnese , Biino Valentina , Zuccotti Gianvincenzo , Calcaterra Valeria
Published: August 2022

COVID-19 restrictions have dramatically reduced the active lifestyle and physical activity (PA) levels in the whole population, a situation that can contribute to weight gain and to develop obesity. To improve physical fitness (PF) in children with obesity during COVID-19 restrictions, sport specialists started to deliver physical training through tele-exercise. For these reasons, the aim of this study was to evaluate the effects of a 12-week online supervised training program in children with obesity on different PF components and PA levels. We enrolled a total of 40 Caucasian children (9 F/31 M; aged 11 ± 1.9 years) with obesity. The data collection consisted of a series of anthropometric measures, the PAQ-C questionnaire, and PF tests, valid and reliable tools to assess PF in children. We used a Wilcoxon’s t-test and a Student’s t-test, as appropriate, to assess the differences before and after the training protocol. A total of 37 patients completed the training protocol and were considered in the analysis. Our results show an improvement in all the PF tests, a reduction in the BMI z-score, the waist circumference, and in the waist-to-height ratio, and an increased PA level. In conclusion, the results of our study show that an online supervised training program is effective to promote PA, improving PF and reducing the BMI z-score in children with obesity.
Obesity Prevalence and Associated Socio-Demographic Characteristics and Health Behaviors in Russia and Norway
Authors: Kholmatova Kamila , Krettek Alexandra , Leon David A. , Malyutina Sofia , Cook Sarah , Hopstock Laila A. , Løvsletten Ola , Kudryavtsev Alexander V.
Published: August 2022

Associations between obesity and socio-demographic and behavioral characteristics vary between populations. Exploring such differences should throw light on factors related to obesity. We examined associations between general obesity (GO, defined by body mass index) and abdominal obesity (AO, defined by waist-to-hip ratio) and sex, age, socio-economic characteristics (education, financial situation, marital status), smoking and alcohol consumption in women and men aged 40–69 years from the Know Your Heart study (KYH, Russia, N = 4121, 2015–2018) and the seventh Tromsø Study (Tromsø7, Norway, N = 17,646, 2015–2016). Age-standardized prevalence of GO and AO was higher in KYH compared to Tromsø7 women (36.7 vs. 22.0% and 44.2 vs. 18.4%, respectively) and similar among men (26.0 vs. 25.7% and 74.8 vs. 72.2%, respectively). The positive association of age with GO and AO was stronger in KYH vs. Tromsø7 women and for AO it was stronger in men in Tromsø7 vs. KYH. Associations between GO and socio-economic characteristics were similar in KYH and Tromsø7, except for a stronger association with living with spouse/partner in KYH men. Smoking had a positive association with AO in men in Tromsø7 and in women in both studies. Frequent drinking was negatively associated with GO and AO in Tromsø7 participants and positively associated with GO in KYH men. We found similar obesity prevalence in Russian and Norwegian men but higher obesity prevalence in Russian compared to Norwegian women. Other results suggest that the stronger association of obesity with age in Russian women is the major driver of the higher obesity prevalence among them compared to women in Norway.
Effects of 90 Min Napping on Fatigue and Associated Environmental Factors among Nurses Working Long Night Shifts: A Longitudinal Observational Study
Authors: Watanabe Kazuhiro , Sugimura Naotaka , Shishido Inaho , Konya Issei , Yamaguchi Shinya , Yano Rika
Published: August 2022

For nurses working long night shifts, it is imperative that they have the ability to take naps to reduce fatigue, and that an appropriate environment is prepared where such naps can be taken. We verified the effects of 90 min napping on fatigue and the associated factors among nurses working 16-h night shifts. We investigated 196-night shifts among 49 nurses for one month. Wearable devices, data logging devices, and questionnaires were used to assess nap parameters, fatigue, and environmental factors such as the napping environment, ways of spending breaks, and working environment. Nurses who nap at least 90 min on most night shifts had more nursing experience. Multivariable logistic regression analysis showed that the environmental factors significantly associated with total nap duration (TND) ≥ 90 min were noise, time spent on electronic devices such as cellphones and tablets during breaks, and nap break duration. The night shifts with TND ≥ 90 min showed lower drowsiness after nap breaks and less fatigue at the end of night shift compared to those with TND < 90 min. Nurses and nursing managers should recognize the importance of napping and make adjustments to nap for at least 90 min during long night shifts.
How Does Government Efficiency Affect Health Outcomes? The Empirical Evidence from 156 Countries
Authors: Ding Yemin , Chin Lee , Li Fangyan , Deng Peidong
Published: August 2022

This paper uses the unbalanced panel data of 156 countries during the period of 2002 to 2018 to explore the possible impact of government efficiency on health outcomes. Firstly, we used the fixed-effect model to examine the relationship between government efficiency and health outcomes and found that the increase in government efficiency can significantly improve health outcomes. Then, a series of robustness checks were carried out, which confirmed the reliability of the above result. Thirdly, this paper conducted a heterogeneity analysis from the perspective of life cycle. Fourthly, this paper investigated the mechanisms of the impact of government efficiency on health outcomes from the perspectives of economic growth, health innovation, education and corruption control. Finally, this paper studied the moderating effects of the ruling party’s ideology and democracy on the relationship between government efficiency and health outcomes. The findings of this study provide some references for governments to improve health outcomes.