bluuebunny/crossref_metadata_2025 huggingface dataset:
Current knowledges in pharmaconutrition: “Ketogenics” in pediatric gliomas
Authors: Cecchi Nicola , Romanelli Roberta , Ricevuti Flavia , Amitrano Marianna , Carbone Maria Grazia , Dinardo Michele , Burgio Ernesto
Published: August 2023

Brain tumors account for 20–25% of pediatric cancers. The most frequent type of brain tumor is Glioma from grade I to grade IV according to the rate of malignancy. Current treatments for gliomas use chemotherapy, radiotherapy, tyrosine kinase inhibitors, monoclonal antibodies and surgery, but each of the treatment strategies has several serious side effects. Therefore, to improve treatment efficacy, it is necessary to tailor therapies to patient and tumor characteristics, using appropriate molecular targets. An increasingly popular strategy is pharmaconutrition, which combines a tailored pharmacological treatment with a diet designed to synergize the effects of drugs. In this review we deal in the molecular mechanisms, the epigenetic effects and modulation of the oxidative stress pathway of ketogenic diets, that underlie its possible role, in the treatment of infantile gliomas, as a complementary approach to conventional cancer therapy.
Prognostic values of geriatric nutrition risk index on elderly patients after spinal tuberculosis surgery
Authors: Huang Yong , Wu Ruibang , Xia Qinghong , Liu Limin , Feng Ganjun
Published: August 2023

Background

Spinal tuberculosis (STB) is a significant public health concern, especially in elderly patients, due to its chronic and debilitating nature. Nutritional status is a critical factor affecting the prognosis of STB patients. The geriatric nutritional risk index (GNRI) has been established as a reliable predictor of adverse outcomes in various diseases, but its correlation with surgical outcomes in elderly STB patients has not been studied.

Objective

The study aimed to assess the prognostic value of the GNRI in elderly patients with STB who underwent surgery.

Methods

We conducted a retrospective analysis of medical records of elderly patients (65 years or older) diagnosed with active STB who underwent surgical treatment. Data collection included patient demographics, comorbidities, clinical history, laboratory testing, and surgical factors. GNRI was calculated using serum albumin levels and body weight. Postoperative complications were observed and recorded. The patients were followed up for at least 1 year, and their clinical cure status was assessed based on predefined criteria.

Results

A total of 91 patients were included in the study. We found that a GNRI value of <98.63 g/dL was a cutoff value for predicting unfavorable clinical prognosis in elderly STB patients undergoing surgery. Patients with a low GNRI had higher Charlson Comorbidity Index scores, were more likely to receive red blood cell transfusions, and had a higher prevalence of overall complications, particularly pneumonia. The unfavorable clinical prognosis group had lower GNRI scores compared to the favorable prognosis group. Multivariate analysis showed that lower GNRI independently predicted unfavorable clinical outcomes in elderly STB patients.

Conclusion

The study concluded that the GNRI is a valuable biomarker for predicting prognosis in elderly STB patients undergoing surgical intervention. Patients with lower GNRI scores had worse outcomes and a higher incidence of complications.
Early-life gut microbiota and neurodevelopment in preterm infants: a narrative review
Authors: Beghetti Isadora , Barone Monica , Brigidi Patrizia , Sansavini Alessandra , Corvaglia Luigi , Aceti Arianna , Turroni Silvia
Published: August 2023

Infants born preterm are at a high risk of both gut microbiota (GM) dysbiosis and neurodevelopmental impairment. While the link between early dysbiosis and short-term clinical outcomes is well established, the relationship with long-term infant health has only recently gained interest. Notably, there is a significant overlap in the developmental windows of GM and the nervous system in early life. The connection between GM and neurodevelopment was first described in animal models, but over the last decade a growing body of research has also identified GM features as one of the potential mediators for human neurodevelopmental and neuropsychiatric disorders. In this narrative review, we provide an overview of the developing GM in early life and its prospective relationship with neurodevelopment, with a focus on preterm infants. Animal models have provided evidence for emerging pathways linking early-life GM with brain development. Furthermore, a relationship between both dynamic patterns and static features of the GM during preterm infants’ early life and brain maturation, as well as neurodevelopmental outcomes in early childhood, was documented. Future human studies in larger cohorts, integrated with studies on animal models, may provide additional evidence and help to identify predictive biomarkers and potential therapeutic targets for healthy neurodevelopment in preterm infants.
Identification of broadly-conserved parasitic nematode proteins that activate immunity
Authors: Rosa Bruce A. , Zarlenga Dante S. , Fournet Valsin M. , Beshah Ethiopia , Hill Dolores E. , Zarlenga Alexander , Yee Angela , Liang Xiaowu , Shandling Adam D. , Oberai Amit , Urban Joseph F. , Mitreva Makedonka
Published: August 2023

Introduction

Soil transmitted nematodes are impediments to human health and agricultural production. Poor anthelmintic efficiencies, the emergence of resistant strains, and the persistence of infective stages highlight the need for more effective control strategies. Parasitic nematodes elicit a Th2-type immune response that most often is not protective. Vaccination has thus far been unsuccessful due to unrealized antigenic characters and unknown mechanisms that nematodes use to circumvent host immunity.

Methods

Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins.

Results

Screening the array with sera and ileal fluid samples from immunized pigs suggested cross-reactivity among homologous proteins and a general activation of immunity. PCA clustering showed that the overall immune responses were altered by immunization, but no substantial changes were observed following direct worm challenge with either Ascaris suum or Trichuris suis.

Discussion

Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. This research strongly supports future studies using a computational, comparative genomics/proteomics approach to produce an effective parasite vaccine.
Effects of infection control measures towards preventing SARS-CoV-2 outbreaks in a German choir boarding school from March 2020 to April 2022
Authors: Lampl Benedikt M. J. , Schöberl Patricia , Atzenbeck Noah , Erdl Michael , Dillitzer Nepomuk , Wallbrecher Jakov , Weigl Marcus , Sauer Michael , Kheiroddin Parastoo , Niggel Jakob , Mauerer Richard , Ambrosch Andreas , Kabesch Michael
Published: August 2023

Background

Singing in a choir was associated with larger outbreaks in the beginning of the SARS-CoV-2 pandemic.

Materials and methods

We report on the effect and acceptance of various infection control measures on the occurrence of SARS-CoV-2 infections in the world famous Domspatzen boys' choir from March 2020 to April 2022.

Results

In addition to basic general hygiene measures, systematic rRT-PCR testing and scientifically approved concepts of distancing during singing were applied. While single infections of choir members could not be avoided, singing-related outbreaks were not observed. Until the Omicron variant emerged, potential transmission of SARS-CoV-2 in the school was limited to only one case. Incidences at the school were never higher than in the comparable general population until then. While the impact of the pandemic on daily life and singing was rated as severe, especially by staff members, most students agreed with the usefulness of protection measures and rated them as acceptable. Students viewed regular testing as the most important tool to increase safety in the school.

Discussion

A bundle of infection control measures including regular testing can prevent outbreaks of SARS-CoV-2 even in the setting of choir singing. Measures are acceptable for choir members if they allow to continue with singing and performing.
Evaluation of the effect of direct-acting antiviral agents on melatonin level and lipid peroxidation in chronic hepatitis C patients
Authors: El-Mahdy Nageh Ahmed , Abou-Saif Sabry , Abd EL hamid Medhat Ismail , Hashem Heba M. , Hammad Mohamed Anwar , Abu-Risha Sally El-Sayed
Published: August 2023

Background: Oxidative stress and its end products, such as malondialdehyde (MDA) play a leading role in the pathogenesis of hepatitis C. Melatonin is a hormone that helps regulate circadian rhythms, which likely play a role in infectious diseases in terms of susceptibility, clinical expression, and outcome.

Objective: The present study was conducted to assess serum malondialdehyde and melatonin levels in patients with chronic hepatitis C infection before and after the intake of direct-acting antivirals.

Method: Forty hepatitis C patients were the subjects of this study. While ten healthy volunteers who matched in age and socioeconomic status served as the control subjects. Malondialdehyde and melatonin were assayed in the serum of the three groups, and the results were statistically analyzed.

Results: Hepatitis C patients had significantly higher malondialdehyde (p < 0.001) but significantly lower melatonin (p < 0.001) as compared to the healthy controls. After 12 weeks of treatment with direct-acting antivirals, the malondialdehyde level decreased significantly (p < 0.001) and the melatonin level increased significantly (p < 0.001). A significant negative correlation between malondialdehyde and melatonin was observed.

Conclusion: The present findings suggest that treatment of hepatitis C patients with Direct-acting antivirals improves liver function parameters and antioxidant profiles.
The immune and metabolic treatment approach of using testosterone on mice models of liver injury
Authors: Amer Johnny , Salhab Ahmad , Snobar Hadeel , Alhabil Yazan
Published: August 2023

Background: Natural killer (NK) cells showed an anti-fibrotic effect; however, their function is thought to be impaired in advanced liver injury. In the current study, we aimed to assess the immune and metabolic impact of testosterone on mice models of liver injury.

Methods: Carbon-tetrachloride induced liver fibrosis male mice models was i.p injected for 2 weeks (acute) and 4 weeks (chronic) (n = 36). Testosterone (4 mg/kg mouse body weight) was injected i.p. following the first week of the acute model of CCl4 and following the second week of the chronic model of CCl4. At the end of the experiments, mice were sacrificed, and serum was collected for assessing liver enzymes of ALT and AST, as well as inflammatory markers of IL-6, metabolic makers of C-peptide levels, and lipid and glucose profiles. Livers were harvested and used for histological assessments for inflammation and fibrosis. Fibrosis profiles from liver extracts, αSMA and Collagen III, were assessed by RT-PCR. Moreover, liver tissue-resident NK cells were isolated and evaluated for their activity by assessing INF-γ and IL-6 receptors using ELISA and flow cytometry, respectively.

Results: Serum ALT, AST, and IL-6, as well as metabolic assessments of cholesterol, triglyceride, C-peptide, fasting blood sugar, and fibrotic profiles, were linearly correlated with disease progressions. Histological characterization of the liver was worsened in the chronic model of liver injury. Testosterone-treated mice exhibit a significant reduction in collagen depositions with less dense fibrosis tissue associated with reduced liver injury enzymes and metabolic markers in both the acute and chronic CCl4 mice models in favor of the latter one (p < 0.05). Moreover, testosterone treatments displayed a significant decrease in serum IL-6 of 2.4-fold (p = 0.0001) and 2.3-fold (p = 0.0003) in the acute and chronic models, respectively (p = 0.002), and data showed an increase in INF-γ release from NK associated with a reduction in their IL-6 receptor expressions (p < 0.05).

Conclusion: Our results indicated effects of testosterone on mediating a decreased expressions of NK IL-6 receptors and consequently inducing their activation; which in part, could explain the amelioration of liver injury. Our data suggest an anti-inflammatory and anti-fibrotic treatment approach of using testosterone for delaying disease progressions.
Risk of herpes zoster associated with JAK inhibitors in immune-mediated inflammatory diseases: a systematic review and network meta-analysis
Authors: Xu Qingling , He Liyuan , Yin Yufeng
Published: August 2023

Objective: Janus kinase (JAK) inhibitors are a novel class of drugs that have shown efficacy in treating immune-mediated inflammatory diseases (IMIDs). However, their safety profile in terms of herpes zoster infection remains unclear. We aimed to evaluate the risk of herpes zoster associated with JAK inhibitors in patients with IMIDs.

Methods: A systematic search of electronic databases was conducted to identify randomized controlled trials (RCTs) that evaluated the safety of JAK inhibitors in patients with IMIDs including inflammatory bowel disease (IBD), rheumatoid arthritis (RA), spondyloarthritis (SpA), psoriasis (PsO), and psoriatic arthritis (PsA). The primary outcome of interest was the incidence of herpes zoster infection. Network meta-analysis was performed to compare the risk of herpes zoster among different JAK inhibitors and placebo.

Results: A network meta-analysis was conducted using data from 47 RCTs including 24,142 patients. In patients with IMIDs, peficitinib 100 mg QD was associated with the highest risk of herpes zoster infection in patients with IMIDs, followed by baricitinib 4 mg QD and upadacitinib 30 mg QD. No difference in herpes zoster risk was found for other JAK inhibitors compared with placebo. Subgroup analysis indicated that higher incidence of herpes zoster was found in patients treated by baricitinib 4 mg QD, peficitinib 100 mg QD, and upadacitinib 30 mg QD only in patients with RA.

Conclusion: Our study suggests that some JAK inhibitors, particularly peficitinib, baricitinib, and tofacitinib, are associated with a higher risk of herpes zoster infection in patients with IMIDs.
Can a personalised music listening intervention decrease agitation in hospitalised patients with dementia? A feasibility trial
Authors: Lee Shanna , Chan Lily , Maddison John
Published: August 2023

Introduction

Agitation is a common manifestation of the behavioural and psychological symptoms of dementia (BPSD). Pharmacotherapy is not the first-line management because of its potential harms, particularly in the elderly. Music as a non-pharmacological intervention for agitation has been explored in residential aged-care facilities, but few studies have been situated in hospitals. This pilot aims to evaluate the feasibility of a personalised music listening intervention for reducing agitation in hospitalised patients with dementia in a metropolitan Geriatric Evaluation and Management (GEM) unit.

Methods

Two-arm randomised control feasibility trial. Eligible patients were assigned to the music intervention or control group, with the intervention group receiving music daily between 15:00–16:00, and agitation levels measured in both groups hourly based on the Pittsburgh Agitation Score (PAS) over 5 days of hospitalisation. Post-trial semi-structured interviews assessed feasibility of the intervention.

Results

Twenty-one patients were recruited over 8 months. Interviews with staff involved indicated that the music intervention was manageable to deliver, assisted engagement with patients which increased efficiency of some clinical tasks, and challenged staff mindset around using psychotropic medication to address agitation. PAS results were inconclusive, because of underpowered numbers in this pilot study.

Conclusion

It is feasible for nursing staff to deliver a personalised music listening intervention to patients with dementia in a geriatric unit of a tertiary hospital, without compromising on usual clinical care.
Mental health across two years of the COVID-19 pandemic: a 5-wave longitudinal study in Germany
Authors: Weber Maxi , Burchert Sebastian , Sijbrandij Marit , Patanè Martina , Pinucci Irene , Renneberg Babette , Knaevelsrud Christine , Schumacher Sarah
Published: August 2023

The COVID-19 pandemic has been negatively associated with mental health. However, little is known about the temporal dynamics of mental health in the longer term of the pandemic. We aimed to investigate symptom levels and changes of depression, anxiety, posttraumatic stress, and loneliness spanning two years of the pandemic; and to examine associated risk factors. This five-wave, longitudinal online study from May 2020 to April 2022 included 636 adults (Mage = 39.5 years, SD = 16.11; 84.1% female) from the German general population who completed the international COVID-19 Mental Health Survey. Symptoms of anxiety (Generalized Anxiety Disorder-7; GAD-7), depression (Patient Health Questionnaire-9; PHQ-9), posttraumatic stress (PTSD Checklist for DSM-5; PCL-5), and loneliness (“Do you feel lonely?”) were assessed using mixed-effects models. Associations with anxiety and depressive symptoms were examined with having children, student status, financial worries, contamination fear, and loneliness. PHQ-9, GAD-7, PCL-5, and loneliness scores overall decreased throughout the two-year period of the pandemic but exhibited an increase during two national lockdowns. Controlled for significant associations with female gender and younger age, increased PHQ-9 and GAD-7 scores were associated with contamination fear, financial worries, and loneliness. No associations were found with having children and student status. Symptoms of depression, anxiety, posttraumatic stress, and loneliness decreased over time but varied along with the dynamics of the pandemic. Longitudinal monitoring of mental health in vulnerable subgroups is required, especially those of younger age, females, and the financially insecure.
Optomyography-based sensing of facial expression derived arousal and valence in adults with depression
Authors: Broulidakis M. John , Kiprijanovska Ivana , Severs Liberty , Stankoski Simon , Gjoreski Martin , Mavridou Ifigeneia , Gjoreski Hristijan , Cox Sophia , Bradwell Daisy , Stone James M. , Nduka Charles
Published: August 2023

Background

Continuous assessment of affective behaviors could improve the diagnosis, assessment and monitoring of chronic mental health and neurological conditions such as depression. However, there are no technologies well suited to this, limiting potential clinical applications.

Aim

To test if we could replicate previous evidence of hypo reactivity to emotional salient material using an entirely new sensing technique called optomyography which is well suited to remote monitoring.

Methods

Thirty-eight depressed and 37 controls (≥18, ≤40 years) who met a research diagnosis of depression and an age-matched non-depressed control group. Changes in facial muscle activity over the brow (corrugator supercilli) and cheek (zygomaticus major) were measured whilst volunteers watched videos varying in emotional salience.

Results

Across all participants, videos rated as subjectively positive were associated with activation of muscles in the cheek relative to videos rated as neutral or negative. Videos rated as subjectively negative were associated with brow activation relative to videos judged as neutral or positive. Self-reported arousal was associated with a step increase in facial muscle activation across the brow and cheek. Group differences were significantly reduced activation in facial muscles during videos considered subjectively negative or rated as high arousal in depressed volunteers compared with controls.

Conclusion

We demonstrate for the first time that it is possible to detect facial expression hypo-reactivity in adults with depression in response to emotional content using glasses-based optomyography sensing. It is hoped these results may encourage the use of optomyography-based sensing to track facial expressions in the real-world, outside of a specialized testing environment.
Advocating for children's surgery within country health plans: lessons from Nigeria and the global stage
Authors: Seyi-Olajide Justina O. , Faboya Omolara , Williams Omolara , Lakhoo Kokila , Ameh Emmanuel A.
Published: August 2023

Background

Despite the growing emphasis on provision of quality safe and affordable surgical care in low- and middle-income countries, and the World Health Assembly resolution 68. 15 on strengthening emergency and essential surgical care and anesthesia as components of universal health coverage, a review of published surgical plans of various countries, revealed a lack of emphasis on children's surgery. Due to the peculiarities of the human resource, infrastructure and equipment required for children's surgery, a lack of deliberate actions and policies targeted at strengthening surgical care for children implies that achieving universal health coverage for children may not be a reality in this setting.

Methods

A baseline assessment of children's surgical capacity was conducted in Nigeria as a part of the National Surgical Obstetrics Anesthesia and Nursing Plan (NSOANP) process. The assessment was done using the World Health Organization (WHO) hospital assessment tool modified for children's surgery (Children Surgical Assessment Tool).

Results

Significant infrastructural gaps were found, with an abysmally low density of pediatric surgeons and anaesthesiologists, poor emergency preparedness, lack of reliable surgical data and non-inclusion of children's surgery in the national strategic health plan. Using the Global Initiative for Children's Surgery's (GICS) Optimal Resources for Children's Surgical Care (OReCS) document and focusing on the strategic goals and priorities, children's surgery was incorporated into the NSOANP. Implementation of the plan is currently ongoing.

Conclusion

From Nigeria's experience, appropriate advocacy and inclusion of children surgery providers in policy making will promote prioritization of children's surgery in country health and surgical plans.
Drift phase resolved diffusive radiation belt model: 2. implementation in a case of random electric potential fluctuations
Authors: Lejosne Solène , Albert Jay M. , Walton Samuel D.
Published: August 2023

In the first part of this work, we highlighted a drift-diffusion equation capable of resolving the magnetic local time dimension when describing the effects of trapped particle transport on radiation belt intensity. Here, we implement these general considerations in a special case. Specifically, we determine the various transport and diffusion coefficients required to solve the drift-diffusion equation for equatorial electrons drifting in a dipole magnetic field in the presence of a specific model of time-varying electric fields. Random electric potential fluctuations, described as white noise, drive fluctuations of trapped particle drift motion. We also run a numerical experiment that consists of tracking trapped particles’ drift motion. We use the results to illustrate the validity of the drift-diffusion equation by showing agreement in the solutions. Our findings depict how a structure initially localized in magnetic local time generates drift-periodic signatures that progressively dampen with time due to the combined effects of radial and azimuthal diffusions. In other words, we model the transition from a drift-dominated regime, to a diffusion-dominated regime. We also demonstrate that the drift-diffusion equation is equivalent to a standard radial diffusion equation once the distribution function is phase-mixed. The drift-diffusion equation will allow for radiation belt modeling with a better spatiotemporal resolution than radial diffusion models once realistic inputs, including localized transport and diffusion coefficients, are determined.
A microbiota-epigenetic circuit controls systematic circadian programs in the gut epithelium
Authors: Ma Junjie , Zhang Jianglin , Kuang Zheng
Published: August 2023

The intestinal microbiota is an important factor that regulates mammalian circadian rhythms and health. We previously reported that the microbiota synchronizes lipid uptake and metabolism in the intestinal epithelium through histone deacetylase 3 (HDAC3). However, the breadth and significance of microbiota-circadian crosstalk in the intestine are not well understood. Here, we show that the gut microbiota programs the rhythmic expression of a broad range of biological processes, and temporally orchestrates epithelial functions and physiology in accordance with the rhythmic gut environment. Protein synthesis, cell proliferation, and metabolic and immune activities are differentially expressed in the daytime and nighttime respectively, indicating a daily alternation of “working” and “recharging” themes in the gut. The rhythms of gene expression are dampened or altered in germ-free mice, suggesting that the microbiota helps to structure the timing of host gene expression. Further analysis showed that HDAC3 drives a vast majority of these microbiota-dependent circadian programs, likely through rhythmic deacetylation of histones. Motif enrichment analysis revealed that HDAC3 could differentially control distinct rhythmic pathways, most likely by recruiting different transcription factors. These findings provide a systematic view of how the commensal microbiota exploits an epigenetic factor to program just-in-time functions in the intestinal epithelium and maintain host homeostasis.
Comparative cryopreservation of bovine and porcine primary hepatocytes
Authors: Andres Sandra , Bartling Babett , Stiensmeier Vera , Starke Alexander , Schmicke Marion
Published: August 2023

The isolation of primary hepatocytes from liver tissue of farm animals yields a very high number of cells, and a part of them can be stored by cryopreservation for future experiments. As no experience exists with the cryopreservation of hepatocytes from cattle, our study aimed at the cryopreservation of bovine hepatocytes by use of different protocols compared with the cryopreservation of hepatocytes from pig. We tested different freezing media (William’s Medium E vs. University of Wisconsin solution), cryoprotectants (dimethyl sulfoxide with vs. without trehalose as additional additive), freezing systems (standard freezing container vs. controlled-rate freezer) and freezing times (4 vs. 28 d). These tests identified a general influence of species and freezing systems, whereas the influence of freezing media, trehalose additive and freezing time was less or not obvious. In this regard, we determined a mean recovery of 30% of bovine hepatocytes and 55% of porcine hepatocytes cryopreserved in a controlled-rate freezer, whereas the rates were about 10% less when hepatocytes were frozen in a standard freezing container. In accordance with this observation, the cultivation of cryopreserved hepatocytes from cattle was less effective than that of porcine hepatocytes. Hepatocytes from cattle can be successfully cryopreserved and partially cultured after cryopreservation but with lower percentage than porcine hepatocytes.
Enhancing Student Wellbeing Through Social Prescribing: A Rapid Realist Review
Authors: Davies Mark , Elliott Megan , Wallace Sarah , Wallace Carolyn
Published: August 2023

Objectives: A Rapid Realist Review of social prescribing in Higher Education (HE) was undertaken to determine what works, for whom, how, why, and within what circumstances. The review resulted in the development of a Realist Programme Theory articulating the way in which social prescribing can be implemented within the HE environment.

Methods: Searches of 12 electronic databases were supplemented by citation chaining and grey literature surfaced by the Project Advisory Group. The RAMESES Quality Standards for Realist Review were followed, and the retrieved articles were systematically screened and iteratively analysed to develop Context-Mechanism-Outcome Configurations (CMOCs) and an overarching Realist Programme Theory.

Results: A total of 57 documents were included. The overarching programme theory was developed from the analysis of these documents and comprised of a social prescribing pathway with the following components: (1) An Accessible Gateway, (2) A Skilled Peer, (3) Trusted-Safe-Credible Resources, and (4) A Healthy Setting.

Conclusion: A Realist Programme Theory was developed—this model and associated principles will provide a theoretical basis for the implementation of social prescribing pathways within higher education. Whilst the direct project outputs are of particular significance to the UK HE audience, the underpinning principles can support practice within the global arena.
Production of Late Seedlings of Açai (Euterpe oleraceae) in an Aquaponic System with Tambaqui (Colossoma macropomum, Curvier, 1818)
Authors: Nascimento Edileno Tiago de Sousa , Pereira Junior Raimundo Formento , Reis Valéria Silva dos , Gomes Bianca de Jesus Figueiredo , Owatari Marco Shizuo , Luz Ronald Kennedy , Melo Nuno Filipe Alves Correia de , Santos Maria de Lourdes Souza , Palheta Glauber David Almeida , Sterzelecki Fabio Carneiro
Published: August 2023

Over the years, aquaponics has become a powerful technological tool that allows the sustainable production of food, integrating conventional fish farming with vegetable production. The present study evaluated the production of late seedlings of açai Euterpe oleraceae in an aquaponic system with tambaqui Colossoma macropomum. A total of 36 tambaquis with an initial average weight and length of 1086.75 ± 16.38 g and 38.49 ± 0.90 cm were distributed in 12 independent aquaponic units, totaling 3.62 kg m−3. The fish were fed three times daily with commercial feed at a rate of 3%. Three flooding levels of 5, 10 and 15 cm, with constant water flow through the hydroponic bed (0.5 m2), were evaluated, and a control-hydroponic bed with flooding levels of 10 cm was established, all in triplicate. In the 5 and 10 cm treatments, 3450 açai seedlings with an initial height of 12.3 ± 1.9 cm were used (575 per aquaponic units), while the 15 cm treatment contained non-germinated açai seeds. The control group did not receive açai seeds and remained empty. Analyses to monitor total dissolved solids (TDS), electrical conductivity, dissolved oxygen, temperature, pH, alkalinity, hardness, nitrogenous compounds, and phosphate levels were performed. At the end of the 30-day trial, the growth performance of tambaqui and plants was evaluated. Water quality was significantly (p < 0.05) affected by different flooding levels. Electrical conductivity and TDS decreased with an increasing in flooding levels. The flooding levels significantly influenced (p < 0.05) the concentration of total ammonia and nitrate between the treatments. The 5 cm flooding level showed the best plant development indexes for total height, aerial portion height, root height and aerial portion fresh mass. No significant differences (p < 0.05) were observed in tambaqui growth performance. The aquaponic system proved to be effective in reusing fish waste (excreta and feed leftovers) generated in the system. The biotransformation of waste into nutrients allowed the growth of plants and nitrifying bacteria, which, through their metabolic pathways, ensured the purification and reuse of water, avoiding the discharge of this waste into the environment.
Recent Progress in Antibody Epitope Prediction
Authors: Zeng Xincheng , Bai Ganggang , Sun Chuance , Ma Buyong
Published: August 2023

Recent progress in epitope prediction has shown promising results in the development of vaccines and therapeutics against various diseases. However, the overall accuracy and success rate need to be improved greatly to gain practical application significance, especially conformational epitope prediction. In this review, we examined the general features of antibody–antigen recognition, highlighting the conformation selection mechanism in flexible antibody–antigen binding. We recently highlighted the success and warning signs of antibody epitope predictions, including linear and conformation epitope predictions. While deep learning-based models gradually outperform traditional feature-based machine learning, sequence and structure features still provide insight into antibody–antigen recognition problems.
Susceptibility of Actinobacillus pleuropneumoniae, Pasteurella multocida and Streptococcus suis Isolated from Pigs in Hungary between 2018 and 2021
Authors: Somogyi Zoltán , Mag Patrik , Simon Réka , Kerek Ádám , Makrai László , Biksi Imre , Jerzsele Ákos
Published: August 2023

Porcine respiratory disease complex (PRDC) has been a major animal health, welfare, and economic problem in Hungary; therefore, great emphasis should be put on both the prevention and control of this complex disease. As antibacterial agents are effective tools for control, antibiotic susceptibility testing is indispensable for the proper implementation of antibacterial therapy and to prevent the spread of resistance. The best method for this is to determine the minimum inhibitory concentration (MIC) by the broth microdilution method. In our study, we measured the MIC values of 164 Actinobacillus pleuropneumoniae, 65 Pasteurella multocida, and 118 Streptococcus suis isolates isolated from clinical cases against the following antibacterial agents: amoxicillin, ceftiofur, cefquinome, oxytetracycline, doxycycline, tylosin, tilmicosin, tylvalosin, tulathromycin, lincomycin, tiamulin, florfenicol, colistin, enrofloxacin, and sulfamethoxazole-trimethoprim. Outstanding efficacy against A. pleuropneumoniae isolates was observed with ceftiofur (100%) and tulathromycin (100%), while high levels of resistance were observed against cefquinome (92.7%) and sulfamethoxazole-trimethoprim (90.8%). Ceftiofur (98.4%), enrofloxacin (100%), florfenicol (100%), and tulathromycin (100%) were found to be highly effective against P. multocida isolates, while 100% resistance was detected against the sulfamethoxazole-trimethoprim combination. For the S. suis isolates, only ceftiofur (100%) was not found to be resistant, while the highest rate of resistance was observed against the sulfamethoxazole-trimethoprim combination (94.3%). An increasing number of studies report multi-resistant strains of all three pathogens, making their monitoring a high priority for animal and public health.
Molecular Mechanisms and Applications of Polyphenol-Protein Complexes with Antioxidant Properties: A Review
Authors: Feng Yulin , Jin Chengming , Lv Shihao , Zhang Huijuan , Ren Feiyue , Wang Jing
Published: August 2023

Proteins have been extensively studied for their outstanding functional properties, while polyphenols have been shown to possess biological activities such as antioxidant properties. There is increasing clarity about the enhanced functional properties as well as the potential application prospects for the polyphenol–protein complexes with antioxidant properties. It is both a means of protein modification to provide enhanced antioxidant capacity and a way to deliver or protect polyphenols from degradation. This review shows that polyphenol–protein complexes could be formed via non-covalent or covalent interactions. The methods to assess the complex’s antioxidant capacity, including scavenging free radicals and preventing lipid peroxidation, are summarized. The combination mode, the type of protein or polyphenol, and the external conditions will be the factors affecting the antioxidant properties of the complexes. There are several food systems that can benefit from the enhanced antioxidant properties of polyphenol–protein complexes, including emulsions, gels, packaging films, and bioactive substance delivery systems. Further validation of the cellular and in vivo safety of the complexes and further expansion of the types and sources of proteins and polyphenols for forming complexes are urgently needed to be addressed. The review will provide effective information for expanding applications of proteins and polyphenols in the food industry.
A Poly-D-Mannose Synthesized by a One-Pot Method Exhibits Anti-Biofilm, Antioxidant, and Anti-Inflammatory Properties In Vitro
Authors: Tian Dandan , Qiao Yu , Peng Qing , Zhang Yuwei , Gong Yuxin , Shi Linbo , Xiong Xiaoyan , He Mengxin , Xu Xiaoqing , Shi Bo
Published: August 2023

In this study, D-mannose was used to synthesize poly-D-mannose using a one-pot method. The molecular weight, degree of branching, monosaccharide composition, total sugar content, and infrared spectrum were determined. In addition, we evaluated the safety and bioactivity of poly-D-mannose including anti-pathogen biofilm, antioxidant, and anti-inflammatory activity. The results showed that poly-D-mannose was a mixture of four components with different molecular weights. The molecular weight of the first three components was larger than 410,000 Da, and that of the fourth was 3884 Da. The branching degree of poly-D-mannose was 0.53. The total sugar content was 97.70%, and the monosaccharide was composed only of mannose. The infrared spectra showed that poly-D-mannose possessed characteristic groups of polysaccharides. Poly-D-mannose showed no cytotoxicity or hemolytic activity at the concentration range from 0.125 mg/mL to 8 mg/mL. In addition, poly-D-mannose had the best inhibition effect on Salmonella typhimurium at the concentration of 2 mg/mL (68.0% ± 3.9%). The inhibition effect on Escherichia coli O157:H7 was not obvious, and the biofilm was reduced by 37.6% ± 2.9% at 2 mg/mL. For Staphylococcus aureus and Bacillus cereus, poly-D-mannose had no effect on biofilms at low concentration; however, 2 mg/mL of poly-D-mannose showed inhibition rates of 33.7% ± 6.4% and 47.5% ± 4%, respectively. Poly-D-mannose showed different scavenging ability on free radicals. It showed the best scavenging effect on DPPH, with the highest scavenging rate of 74.0% ± 2.8%, followed by hydroxyl radicals, with the scavenging rate of 36.5% ± 1.6%; the scavenging rates of superoxide anion radicals and ABTS radicals were the lowest, at only 10.1% ± 2.1% and 16.3% ± 0.9%, respectively. In lipopolysaccharide (LPS)-stimulated macrophages, poly-D-mannose decreased the secretion of nitric oxide (NO) and reactive oxygen species (ROS), and down-regulated the expression of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). Therefore, it can be concluded that poly-D-mannose prepared in this research is safe and has certain biological activity. Meanwhile, it provides a new idea for the development of novel prebiotics for food and feed industries or active ingredients used for pharmaceutical production in the future.
Potential Antioxidant and Anti-Inflammatory Effects of Lonicera japonica and Citri Reticulatae Pericarpium Polyphenolic Extract (LCPE)
Authors: Jeong Se Hyo , Park Min Yeong , Bhosale Pritam Bhagwan , Abusaliya Abuyaseer , Won Chung Kil , Park Kwang Il , Kim Eunhye , Heo Jeong Doo , Kim Hyun Wook , Ahn Meejung , Seong Je Kyung , Kim Hun Hwan , Kim Gon Sup
Published: August 2023

Dermatitis is an inflammatory condition of the outer layer of the skin that causes itching, blisters, redness, swelling, and often exudation, scabs, and peeling. Among them, purulent inflammation is a symptom that often occurs on the skin and appears in the form of boils and acne. Various studies are being conducted to treat these inflammatory diseases. Accordingly, Lonicera japonica and Citri Reticulatae Pericarpium Polyphenolic Extract (LCPE), which uses herbal preparations such as Lonicera japonica, Citri Reticulatae Pericarpium, and Glycyrrhiza uralensis, has been used to suppress inflammation since ancient times, and its anti-inflammatory effect can be observed in skin keratinocytes after inducing inflammation. In this study, the major polyphenolic compounds in LCPE were quantitatively determined by analyzing the data through peak values using high-performance chromatography (HPLC-MS/MS) coupled with mass spectrometry. Additionally, bioactive compounds targeting 2,2-diphenyl-1-picrylhydrazyl (DPPH) were analyzed by ultrafiltration integrated with LC. Several compounds with the most significant effects were selected (chlorogenic acid, narirutin, and isorhamnetin). Skin keratinocytes induced by lipopolysaccharide (LPS) were treated with LCPE to show its anti-inflammatory effects. After LCPE treatment, inflammation-mediating cytokines such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) were decreased. In addition, nuclear factor kappa (NF-кB) and mitogen-activated protein kinase (MAPK) were inhibited in important pathways related to inflammation. Lastly, molecular modeling was performed to determine binding scores with inflammation-related proteins using molecular docking for the selected compounds. According to these results, LCPE is effective in treating keratinocytes induced by LPS and reducing inflammation and has potential antioxidant effects, and the polyphenol components have been identified.
Surface Quality of Al2O3 Ceramic and Tool Wear in Diamond Wire Sawing Combined with Oil Film-Assisted Electrochemical Discharge Machining
Authors: Jia Zhixin , Zhang Kaiyue , Wang Jin
Published: August 2023

Diamond wire sawing is one of the most widely used methods of cutting Al2O3 ceramic because it has good machining accuracy and causes less surface damage. However, its material removal rate (MRR) needs to be improved with the increasing demand for Al2O3 ceramic parts. In this paper, spark discharges are generated around the diamond wire based on the electrochemical discharge machining (ECDM) process. An oil film-assisted ECDM process is applied to solve the difficulty of generating spark discharges when the thickness of the workpiece exceeds 5.0 mm due to the difficulty of forming a hydrogen gas film. Experimental results show that the combination of oil film-assisted ECDM and diamond wire sawing improved the MRR of Al2O3 ceramic. Oil film-assisted ECDM may improve the surface quality of machined parts and reduce the wear on diamond wire. Therefore, this research focuses on the surface quality of Al2O3 ceramic and tool wear in diamond wire sawing combined with oil film-assisted ECDM. Surface roughness and topography, recast layer, and elements of the machined surface are analyzed. The tool wear is studied using SEM images of diamond wire. The results provide a valuable basis for application of diamond wire sawing combined with oil film-assisted ECDM.
Thermochemical Analysis of Hydrogenation of Pd-Containing Composite Based on TiZrVNbTa High-Entropy Alloy
Authors: Savvotin Ivan , Berdonosova Elena , Korol Artem , Zadorozhnyy Vladislav , Zadorozhnyy Mikhail , Statnik Evgeniy , Korsunsky Alexander , Serov Mikhail , Klyamkin Semen
Published: August 2023

The microcalorimetric hydrogen titration technique combined with conventional volumetric measurements has been used to reveal peculiarities of the hydrogenation of the single-phase TiZrVNbTa equiatomic high-entropy alloy. The alloy has been produced in the form of microfibers by the pendent drop melt extraction technique. Palladium coating of the fibers has been applied to enable first hydrogenation at room temperature without additional activation. An analysis of the obtained data allows us to evaluate the dependence of hydrogenation enthalpy on the hydrogen concentration in the alloy. Three concentration ranges, presumably related to the formation of the hydrogen solid solution, monohydride and dihydride phases, have been identified, and the corresponding ΔH values of about −100, −80 and −60 kJ/mol H2, respectively, have been determined.
Parametric Study on the Aerodynamic Characteristics of Wind Guide Barriers for a Train–Bridge System
Authors: Guo Dianyi , Jiang Shuo , Zou Yunfeng , He Xuhui , Liu Qingkuan
Published: August 2023

This paper presents a new type of wind guide barrier (WGB). Through wind tunnel experiments, the key parameters of three types of WGB were studied, as well as their effects on the aerodynamic characteristics of a high-speed train and blunt body box girder system, while the bridge deck wind field was investigated. The results show that the shielding effect is the main factor affecting the windproof performance of the WGB. When the WGB is installed, the mean wind pressure distribution on the train is comparable to that of using a vertical wind barrier while reducing barrier resistance and the resistance of the bridge–barrier system. At the same time, the WGB can guide the oncoming flow, resulting in an increase in the streamwise and vertical wind velocity ratios on the bridge deck, as well as an increase in the maximum aerodynamic coefficient on the train.
A Study Utilizing Numerical Simulation and Experimental Analysis to Predict and Optimize Flange-Forming Force in Open-Die Forging of C45 Billet Tubes
Authors: Phan Thi-Ha-Linh , Luyen The-Thanh , Nguyen Duc-Toan
Published: August 2023

Open-die forging holds a pivotal role in shaping machine parts within industrial applications. This study focuses on the assessment of stress–strain curves for C45 material at different elevated temperatures and strain rates through numerical simulations employing the finite element method (FEM). Specifically, the research investigates how the flow curve of materials at elevated temperatures and individual strain rates impacts the forming force during the flange forming of C45 billet tubes. By comparing the simulation results with experimental data on the flange-forming force, this study observes that optimal outcomes arise when considering both elevated temperature and strain rates in the flow curve of materials. The study then conducts simulations for C45 billet tubes with varying upsetting ratios (H0/D0), (S0/D0), and the punch’s pitch angle (α), aiming to address optimization challenges related to the flange-forming force. Consequently, a mathematical model is developed to represent the relationship between the flange-forming force and geometric parameters (H0/D0, S0/D0, and α). This model accurately predicts the forming force under various flange-forming conditions, demonstrating high precision with a maximum error of 4.26% compared with the experimental results. This study significantly contributes to the advancement of flange-forming technology in open-die forging through numerical simulation, enabling the optimization of the flange-forming force and the selection of appropriate equipment. These findings pave the way for more effective and efficient industrial processes, fostering innovation and progress in the field.