bluuebunny/crossref_metadata_2025 huggingface dataset:
Earthquake scenario-specific framework for spatial accessibility analysis (SAA) of emergency shelters: a case study in Xichang City, Sichuan Province, China
Authors: Wang Ziyue , Cheng Jia , Xu Chong
Published: April 2024

The spatial accessibility of emergency shelters, indicating the difficulty of evacuation and rescue, is crucial for disaster mitigation and emergency management. To analyze accessibility, an effective approach is to evaluate the service capacity of emergency shelters. Multifaceted factors were employed to enhance the quantitative accuracy of accessibility indicators. However, scenario-specific analysis has not been emphasized. Considering the devastating potential of great earthquake disasters, we cannot ignore the impact of these scenarios on emergency shelter accessibility, especially in areas with high seismic risk. In this study, we developed an earthquake scenario-specific framework for spatial accessibility analysis (SAA), which integrates the service capacity of emergency shelters and the impact of strong ground motion and fault rupturing. We applied this framework to the urban area of Xichang City in Sichuan Province, western China. Xichang City, located in the linked area of the Anninghe fault and Zemuhe fault with many extreme historical earthquake disaster records, is prone to high seismic risk. We firstly collected emergency shelter and road network data in Xichang City. We then applied SAA based on the road network, using the network analysis method. After that, we analyzed the impact of strong ground motion on accessibility and generated the setback zone of fault rupturing. We integrated the effect of strong ground motion on accessibility within the setback zone of active faults. Finally, we generated a comprehensive accessibility map, considering both the predicted strong ground motion and potential fault rupturing. Our results show that the accessibility level changed in several towns of urban Xichang City due to strong ground motion and fault rupturing. The accessibility level decreased in Lizhou, Xingsheng, and Anning Towns. For areas with mapped fault lines, the accessibility level is Very-Low. Our results demonstrate the impact of earthquake damage on the accessibility of emergency shelters and the complexity of evacuation in earthquake scenarios. In general, we added earthquake rupturing and ground motion characteristics into the SAA framework. This framework will help us enhance the reliability of SAA and the feasibility of seismic vulnerability evaluation.
Effects of short-term simulated acid rain and nitrogen deposition on soil nutrients and enzyme activities in Cunninghamia lanceolata plantation
Authors: Ding Yong , Sun Lianhao , Li Chong , Chen Meiling , Zhou Yuexiang , Meng Miaojing , Li Zhenghao , Zhang Jinchi , Liu Xin
Published: April 2024

Acid rain and nitrogen deposition are emerging as global scale environmental issues due to increasing industrial emissions and agricultural pollutants, which seriously impac t the sustainable development of global ecosystems. However, the specific effects both acid rain and nitrogen deposition interactions on forest soil ecosystems, particularly as relates to the soil nutrient content and enzyme activities, remain unclear. Therefore, we established a simulated sample plot of acid rain (SR, NR) and nitrogen deposition (N) and their interactions (SRN, NRN) in a subtropical Cunninghamia lanceolata (C. lanceolata) plantation in the Yangtze River Delta region of China to investigate the impacts of these factors via correlation analysis and structural equation model (SEM). The results showed that acid rain had a stronger effect on soil pH than nitrogen deposition in C. lanceolata plantation, while the simultaneous addition of acid rain and nitrogen deposition exacerbated soil acidification. Soil available potassium, ammonium nitrogen and nitrate nitrogen in C. lanceolata plantation responded more obviously to acid rain and nitrogen deposition, in which acid rain, nitrogen deposition and their interactions significantly reduced soil available potassium content, while acid rain and nitrogen deposition interactions significantly increased soil ammonium nitrogen and nitrate nitrogen content. Nitric acid rain, nitrogen deposition and their interactions significantly increased soil NAGase activity, but significantly decreased soil urease activity; the single-factor treatment of acid rain and nitrogen deposition significantly increased soil arylsulfatase activity, while the interaction of acid rain and nitrogen deposition significantly decreased soil arylsulfatase activity; in general, the interaction of acid rain and nitrogen deposition had a stronger effect on the soil ecosystem of the C. lanceolata plantation than that of single acid rain or nitrogen deposition, of which nitrogen deposition exacerbated the effects of acid rain on the soil ecosystem of C. lanceolata plantation mainly by changing the soil pH and the content of effective nutrients.
High adherence to intermittent and continuous use of a contraceptive vaginal ring among women in a randomized controlled trial in Kigali, Rwanda
Authors: Kestelyn Evelyne , Van Nuil Jennifer Ilo , Umulisa Marie Michelle , Umutoni Grace , Uwingabire Alice , De Baetselier Irith , Uwineza Mireille , Agaba Stephen , Crucitti Tania , Delvaux Thérèse , van de Wijgert Janneke H. H. M.
Published: April 2024

Background

The introduction of female-initiated drug-delivery methods, including vaginal rings, have proven to be a promising avenue to address sexually transmitted infections and unintended pregnancies, which disproportionally affects women and girls in sub-Saharan Africa. Efficient uptake of existing and new technologies such as vaginal rings requires in depth understanding of product adherence. This remains a major challenge as data on adherence to vaginal rings from African countries is limited. In this study, we explored adherence of contraceptive vaginal ring (NuvaRing®) use in Kigali, Rwanda using a mixed methods approach.

Methods

We collected quantitative and qualitative data at multiple time points from women participating in a clinical trial exploring the safety and acceptability of either intermittent or continuous use of the NuvaRing®. Various adherence categories were used including monthly and cumulative adherence measurement. The quantitative data were analysed using R and the qualitative data were analysed using a deductive, content-analytical approach based on categories related to the quantitative adherence measures. All data were compared and triangulated.

Results

Data from 120 enrolled participants showed that self-reported adherence was high at every study visit in both study groups. At first study visit 80% of the intermittent ring users and 79.7% of the continuous ring users reported perfect adherence (assessed as “the ring was never out”). Reporting of ring expulsions and removals were highest (28.3%) at the beginning of the trial. Self-reported perfect ring adherence increased during the study and reports of ring expulsions and removals declined as familiarity with this contraceptive method increased. The percentage of women with perfect cumulative adherence was non-significantly higher in the intermittent (61.7%) than in the continuous use group (54.3%). The low rate of discrepant adherence data after triangulation (6%) is in line with the perception of the participants as adherent throughout the study.

Conclusions

Self-reported adherence in both study groups was high with removals and expulsions being within the expected product range. Comprehensive adherence data triangulation allowed for a deeper understanding of context-driven behaviour that shaped adherence patterns and challenges. Our data categorisation and triangulation approach has shown potential for implementation in future vaginal ring studies aiming to better understand and measure adherence.
Production and verification of novel Osmanthus hybrids
Authors: Alexander Lisa , Wu Xingbo , Gouker Fred
Published: April 2024

Osmanthus fragrans Lour. is prized for its fragrant, cool-season blooms, smooth leaves, and compact tree form; however, the species is limited in production and use to USDA Hardiness Zones 7 and higher. Increasing the total amount of genetic variability available for evaluation, selection, and breeding is foundational to the development of novel Osmanthus cultivars with increased cold-hardiness and improved ornamental characteristics such as high rooting rates, faster growth, fragrant flowers, and an evergreen or semi-evergreen habit. Hybridization among Osmanthus species should generate novel phenotypes and allow trait introgression among populations and breeding lines. The objectives of this study were to produce, verify, and evaluate Osmanthus hybrids by making controlled and open-pollinated crosses among Osmanthus species, using SSR markers to determine parentage of cross progeny, and comparing flowering, leaf shape, and form of hybrids offspring with parents. Fifty-eight of 66 progenies (88%) were assigned paternity at the 95% confidence level or above and 47 progenies (71%) were assigned paternity at the 99% confidence level. Novel Osmanthus hybrids were produced including O. armatus × O. fortunei, O. armatus × O. heterophyllus, and O. armatus × O. fragrans. Open-pollinated families increased in height an average of 204% and 159% per year in the first and second growing seasons, respectively. Male flower type was significantly associated with progeny flower type (χ2 = 17.5, p < 0.0001) while leaf margin was not associated with cross family (χ2 = 0.83, p = 0.84). Evaluation of more Osmanthus accessions and subsequent hybridizations will be necessary to introgress favorable genes and generate the variation necessary to expand the genus into new markets.
The predictive value of next generation sequencing for matching advanced hepatocellular carcinoma patients to targeted and immunotherapy
Authors: Du Jiajia , Zhang Erlei , Huang Zhiyong
Published: April 2024

Background

Targeted and Immunotherapy has emerged as a new first-line treatment for advanced hepatocellular carcinoma (aHCC). To identify the appropriate targeted and immunotherapy, we implemented next generation sequencing (NGS) to provide predictive and prognostic values for aHCC patients.

Methods

Pretreatment samples from 127 HCC patients were examined for genomic changes using 680-gene NGS, and PD-L1 expression was detected by immunohistochemistry. Demographic and treatment data were included for analyses of links among treatment outcomes, drug responses, and genetic profiles. A prognostic index model for predicting benefit from treatment was constructed, taking into account of biomarkers, including TP53, TERT, PD-L1, and tumor mutation burden (TMB) as possible independent prognostic factors.

Results

The multivariate Cox regression analyses showed that PD-L1≥1% (HR 25.07, 95%CI 1.56 - 403.29, p=0.023), TMB≥5Mb (HR 86.67, 95% CI 4.00 - 1876.48, p=0.004), TERT MU (HR 84.09, 95% CI 5.23 - 1352.70, p=0.002) and TP53 WT (HR 0.01, 95%CI 0.00 - 0.47, p=0.022) were independent risk factors for overall survival (OS), even after adjusting for various confounders. A prognostic nomogram for OS was developed, with an area under the ROC curve of 0.91, 0.85, and 0.98 at 1-, 2-, and 3- year, respectively, and a prognostic index cutoff of 1.2. According to the cutoff value, the patients were divided into the high-risk group (n=29) and low-risk group (n=98). The benefit of targeted and immunotherapy in the low-risk group was not distinguishable according to types of agents. However, treatment of Atezolizumab and Bevacizumab appeared to provide longer OS in the high-risk group (12 months vs 9.2, 9, or 5 months for other treatments, p<0.001).

Conclusion

The prognostic model constructed by PD-L1, TMB, TERT, and TP53 can identify aHCC patients who would benefit from targeted and immunotherapy, providing insights for the personalized treatment of HCC.
Neuroretinal degeneration in a mouse model of systemic chronic immune activation observed by proteomics
Authors: Khan Asif Manzoor , Steffensen Maria Abildgaard , Paskeviciute Egle , Abduljabar Ahmed Basim , Sørensen Torben Lykke , Vorum Henrik , Nissen Mogens Holst , Honoré Bent
Published: April 2024

Blindness or vision loss due to neuroretinal and photoreceptor degeneration affects millions of individuals worldwide. In numerous neurodegenerative diseases, including age-related macular degeneration, dysregulated immune response-mediated retinal degeneration has been found to play a critical role in the disease pathogenesis. To better understand the pathogenic mechanisms underlying the retinal degeneration, we used a mouse model of systemic immune activation where we infected mice with lymphocytic choriomeningitis virus (LCMV) clone 13. Here, we evaluated the effects of LCMV infection and present a comprehensive discovery-based proteomic investigation using tandem mass tag (TMT) labeling and high-resolution liquid chromatography–tandem mass spectrometry (LC-MS/MS). Changes in protein regulation in the posterior part of the eye, neuroretina, and RPE/choroid were compared to those in the spleen as a secondary lymphoid organ and to the kidney as a non-lymphoid but encapsulated organ at 1, 8, and 28 weeks of infection. Using bioinformatic tools, we found several proteins responsible for maintaining normal tissue homeostasis to be differentially regulated in the neuroretina and the RPE/choroid during the degenerative process. Additionally, in the organs we observed, several important protein pathways contributing to cellular homeostasis and tissue development were perturbed and associated with LCMV-mediated inflammation, promoting disease progression. Our findings suggest that the response to a systemic chronic infection differs between the neuroretina and the RPE/choroid, and the processes induced by chronic systemic infection in the RPE/choroid are not unlike those induced in non-immune-privileged organs such as the kidney and spleen. Overall, our data provide detailed insight into several molecular mechanisms of neuroretinal degeneration and highlight various novel protein pathways that further suggest that the posterior part of the eye is not an isolated immunological entity despite the existence of neuroretinal immune privilege.
The predictive value of T-cell chimerism for disease relapse after allogeneic hematopoietic stem cell transplantation
Authors: Li Zhipeng , Wang Jing , Deng Lei , Liu Ximin , Kong Fanjun , Zhao Yuerong , Hou Yixi , Zhou Fang
Published: April 2024

Introduction

Chimerism is closely correlated with disease relapse after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, chimerism rate is dynamic changes, and the sensitivity of different chimerism requires further research.

Methods

To investigate the predictive value of distinct chimerism for relapse, we measured bone marrow (BM), peripheral blood (PB), and T-cell (isolated from BM) chimerism in 178 patients after allo-HSCT.

Results

Receiver operating characteristic (ROC) curve showed that T-cell chimerism was more suitable to predict relapse after allo-HSCT compared with PB and BM chimerism. The cutoff value of T-cell chimerism for predicting relapse was 99.45%. Leukemia and myelodysplastic syndrome (MDS) relapse patients’ T-cell chimerism was a gradual decline from 2 months to 9 months after allo-HSCT. Higher risk of relapse and death within 1 year after allo-HSCT. The T-cell chimerism rates in remission and relapse patients were 99.43% and 94.28% at 3 months after allo-HSCT (P = 0.009), 99.31% and 95.27% at 6 months after allo-HSCT (P = 0.013), and 99.26% and 91.32% at 9 months after allo-HSCT (P = 0.024), respectively. There was a significant difference (P = 0.036) for T-cell chimerism between early relapse (relapse within 9 months after allo-HSCT) and late relapse (relapse after 9 months after allo-HSCT) at 2 months after allo-HSCT. Every 1% increase in T-cell chimerism, the hazard ratio for disease relapse was 0.967 (95% CI: 0.948–0.987, P<0.001).

Discussion

We recommend constant monitoring T-cell chimerism at 2, 3, 6, and 9 months after allo-HSCT to predict relapse.
Investigating the influence of sub-mesoscale current structures on Baltic Sea connectivity through a Lagrangian analysis
Authors: Hariri Saeed , Meier H. E. Markus , Väli Germo
Published: April 2024

This study explores the impact of sub-mesoscale structures and vertical advection on the connectivity properties of the Baltic Sea using a Lagrangian approach. High-resolution flow fields from the General Estuarine Transport Model (GETM) were employed to compute Lagrangian trajectories, focusing on the influence of fine-scale structures on connectivity estimates. Six river mouths in the Baltic Sea served as initial positions for numerical particles, and trajectories were generated using flow fields with varying horizontal resolutions: 3D trajectories with 250m resolution as well as 2D trajectories with 250m and 1km resolutions. Several Lagrangian indices, such as mean transit time, arrival depths, and probability density functions of transit times, were analyzed to unravel the complex circulation of the Baltic Sea and highlight the substantial impact of sub-mesoscale structures on numerical trajectories. Results indicate that in 2D simulations, particles exhibit faster movement on the eastern side of the Gotland Basin in high-resolution compared to coarse-resolution simulations. This difference is attributed to the stronger coastal current in high-resolution compared to coarse-resolution simulations. Additionally, the study investigates the influence of vertical advection on numerical particle motion within the Baltic Sea, considering the difference between 3D and 2D trajectories. Findings reveal that denser water in the eastern and south-eastern areas significantly affects particle dispersion in 3D simulations, resulting in increased transit times. Conversely, regions in the North-western part of the basin accelerate particle movement in 3D compared to the 2D simulations. Finally, we calculated the average residence time of numerical particles exiting the Baltic Sea through the Danish strait. Results show an average surface layer residence time of approximately 790 days over an eight-year integration period, highlighting the relatively slow water circulation in the semi-enclosed Baltic Sea basin. This prolonged residence time emphasizes the potential for the accumulation of pollutants. Overall, the study underscores the pivotal role of fine-scale structures in shaping the connectivity of the Baltic Sea, with implications for understanding and managing environmental challenges in this unique marine ecosystem.
Nitrate fate in coastal unconfined aquifers influenced by preferential flows
Authors: Gao Chao , Kong Jun , Wang Jun , Chen Weilun
Published: April 2024

This study examined the influence of preferential flow on pore water flows and marine nitrogen transport reaction in variable saturation and variable density coastal aquifers. The 2-D unconfined aquifer model established was based on the software COMSOL by coupling the dynamic and chemical processes together. The results showed that preferential flow affects groundwater flow and salinity distribution, leading to a more complicated mixing process. The preferential flow resulted in an increase in mixing zone area and the upper saline plume area of 10.33 and 2.62 m2, respectively, a decrease in saltwater wedge area of 7.22 m2, and an increase in nitrate (NO3-) removal efficiency from 7.9% to 8.97%. The NO3- removal efficiency increases progressively with the depth (h) and quantity (n) of preferential flows; however, it decreases after a certain quantity. Further quantitative analysis revealed an increase in the intensity of nitrification and dissolved oxygen inflow flux with preferential flow depth and quantity increase. This phenomenon usually occurs on coasts where biological caves are abundant. The results also offer significant implications for designing engineering measures to mitigate saltwater intrusion and are significant to prevent groundwater quality deterioration in coastal zones.
A practical guide to the management of immune thrombocytopenia co-existing with acute coronary syndrome
Authors: Rahhal Alaa , Provan Drew , Ghanima Waleed , González-López Tomás José , Shunnar Khaled , Najim Mostafa , Ahmed Ashraf Omer , Rozi Waail , Arabi Abdulrahman , Yassin Mohamed
Published: April 2024

Introduction

Immune thrombocytopenia (ITP) management with co-existing acute coronary syndrome (ACS) remains challenging as it requires a clinically relevant balance between the risk and outcomes of thrombosis and the risk of bleeding. However, the literature evaluating the treatment approaches in this high-risk population is scarce.

Methods and Results

In this review, we aimed to summarize the available literature on the safety of ITP first- and second-line therapies to provide a practical guide on the management of ITP co-existing with ACS. We recommend holding antithrombotic therapy, including antiplatelet agents and anticoagulation, in severe thrombocytopenia with a platelet count < 30 × 109/L and using a single antiplatelet agent when the platelet count falls between 30 and 50 × 109/L. We provide a stepwise approach according to platelet count and response to initial therapy, starting with corticosteroids, with or without intravenous immunoglobulin (IVIG) with a dose limit of 35 g, followed by thrombopoietin receptor agonists (TPO-RAs) to a target platelet count of 200 × 109/L and then rituximab.

Conclusion

Our review may serve as a practical guide for clinicians in the management of ITP co-existing with ACS.
Accuracy of toric intraocular lens power calculation depending on different keratometry values using a novel network based software platform
Authors: Ramsauer Michaela , Luft Nikolaus , Vounotrypidis Efstathios , Priglinger Siegfried G. , Mayer Wolfgang J.
Published: April 2024

Purpose

To compare different corneal keratometry readings (swept-source-OCT-assisted biometry and Scheimpflug imaging) with a novel software platform for calculation of toric intraocular lenses.

Setting

Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.

Design

Retrospective, non-randomized, clinical trial.

Methods

Twenty-three eyes undergoing toric intraocular lens implantation were included. Inclusion criteria were preoperative regular corneal astigmatism of at least 1.00 D, no previous refractive surgery, no ocular surface diseases and no maculopathies. Lens exchange was performed with CALLISTO eye (Zeiss). For each patient, the expected postoperative residual refraction was calculated depending on three different corneal parameters of two different devices: standard K-front (K) and total keratometry (TK) obtained by a swept-source-OCT-assisted biometry system (IOL Master 700, Zeiss) as well as total corneal refractive power (TCRP) obtained by a Scheimpflug device (Pentacam AXL, Oculus). Barrett’s formula for toric intraocular lenses was used for all calculations within a novel software platform (EQ workplace, Zeiss FORUM®). Results were statistically compared with postoperative refraction calculated according to the Harris dioptric power matrix.

Results

The standard K values (mean PE 0.02 D ± 0.45 D) and TK values (mean PE 0.09 D ± 0.43 D) of the IOL Master 700 reached similar results (p = 0.96). 78% of eyes in both K and TK groups achieved SE within ±0.5 D of attempted correction and all eyes (100%) were within ±1.0 D of attempted correction in both groups. By contrast, the prediction error in the IOL calculation using the TCRP of the Scheimpflug device was significantly greater (mean PE −0.56 D ± 0.49 D; p = 0.00 vs. standard K and p = 0.00 vs. TK) with adjusted refractive indices. Thirty-nine and Ninety-one percentage of eyes in the TCRP group achieved SE within ±0.5 D (p = 0.008 K vs. TCRP and p = 0.005 TK vs. TCRP) and ± 1.0 D (p = 0.14 vs. TCRP) of attempted correction, respectively.

Conclusion

All three corneal parameters (standard K, TK, TCRP) performed well in calculating toric IOLs. The most accurate refractive outcomes in toric IOL implantation were achieved by IOL calculations based on swept-source-OCT-assisted biometry. The SS-OCT-based K-front and TK values achieve comparable results in the calculation of toric IOLs.
OxyHbMeter—a novel bedside medical device for monitoring cell-free hemoglobin in the cerebrospinal fluid—proof of principle
Authors: Tachatos Nikolaos , Willms Jan Folkard , Gerlt Michael Sebastian , Kuruvithadam Kiran , Hugelshofer Michael , Akeret Kevin , Deuel Jeremy , Keller Emanuela , Schmid Daners Marianne
Published: April 2024

Delayed cerebral ischemia (DCI) occurs in up to one third of patients suffering from aneurysmal subarachnoid hemorrhage (aSAH). Untreated, it leads to secondary cerebral infarctions and is frequently associated with death or severe disability. After aneurysm rupture, erythrocytes in the subarachnoid space lyse and liberate free hemoglobin (Hb), a key driver for the development of DCI. Hemoglobin in the cerebrospinal fluid (CSF-Hb) can be analyzed through a two-step procedure of centrifugation to exclude intact erythrocytes and subsequent spectrophotometric quantification. This analysis can only be done in specialized laboratories but not at the bedside in the intensive care unit. This limits the number of tests done, increases the variability of the results and restricts accuracy. Bedside measurements of CSF-Hb as a biomarker with a point of care diagnostic test system would allow for a continuous monitoring for the risk of DCI in the individual patient. In this study, a microfluidic chip was explored that allows to continuously separate blood particles from CSF or plasma based on acoustophoresis. An in vitro test bench was developed to test in-line measurements with the developed microfluidic chip and a spectrometer. The proof of principle for a continuous particle separation device has been established with diluted blood and CSF samples from animals and aSAH patients, respectively. Processing 1 mL of blood in our microfluidic device was achieved within around 70 min demonstrating only minor deviations from the gold standard centrifugation (7% average error of patient samples), while saving several hours of processing time and additionally the reduction of deviations in the results due to manual labor.
Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils
Authors: Liu Chaoqun , Wang Yinquan , Jin Ling , Wang Yan , Liu Dongling
Published: April 2024

Lanzhou lily (Lilium davidii var. willmottiae) is an exclusive sweet lily variety indigenous to China, which is susceptible to bulbous rot caused by fungal infection during storage. This experiment tests the pathogenicity of the pure culture isolated from the diseased tissue was confirmed in accordance with Koch's postulates, and the pathomycetes were identified based on their morphological and molecular characteristics. Furthermore, the biological characteristics of the pathogens were investigated, followed by an evaluation of the antifungal effects of three plant essential oils against them. The results showed that two strains of fungi were isolated from Lanzhou lily rot, which were identified as Fusarium oxysporum Schl. and Aspergillus sydowii (Bain. Et sart.). In addition, the pathogenicity of these two strains of fungi was demonstrated that only F. oxysporum induced rot with similar symptoms during the post-harvest storage period. The biological characteristics of F. oxysporum indicated the potato maltose agar and lily dextrose agar were identified as the most suitable media. Sucrose was determined to be the optimal carbon source, while ammonium nitrate was found to be the best nitrogen source for the growth of F. oxysporum. Mycelial growth and sporulation of F. oxysporum occurred at an optimum pH value of 6. Total darkness facilitated mycelial growth and conidial germination. The ideal temperature for growth was found to be 28°C, while relative humidity did not significantly impact mycelial growth; however, a relative humidity of 55% was most favorable for spore production. Among the three essential oils tested, cinnamon essential oil displayed superior antifungal efficacy against F. oxysporum, whereas angelica essential oil and tea tree essential oil also exhibited moderate inhibitory effects against this pathogen. This research provides valuable theoretical insights for disease control during the storage and transportation of Lanzhou lily.
Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment
Authors: Chen Liying , Li Dai , Shen Yawei , Li Zhuo , Hao Huanhuan , Ke Caihuan , Meng Zhang , Feng Danqing
Published: April 2024

Research on the microbiota associated with marine invertebrates is important for understanding host physiology and the relationship between the host and the environment. In this study, the microbiota of the green mussel Perna viridis was characterized at the tissue scale using 16S rRNA gene high-throughput sequencing and compared with the microbiota of the surrounding environment. Different mussel tissues were sampled, along with two environmental samples (the mussel's attachment substratum and seawater). The results showed that the phyla Proteobacteria, Bacteroidetes, and Spirochaetae were dominant in mussel tissues. The bacterial community composition at the family level varied among the tissues of P. viridis. Although the microbiota of P. viridis clearly differed from that of the surrounding seawater, the composition and diversity of the microbial community of the foot and outer shell surface were similar to those of the substratum, indicating their close relationship with the substratum. KEGG prediction analysis indicated that the bacteria harbored by P. viridis were enriched in the degradation of aromatic compounds, osmoregulation, and carbohydrate oxidation and fermentation, processes that may be important in P. viridis physiology. Our study provides new insights into the tissue-scale characteristics of mussel microbiomes and the intricate connection between mussels and their environment.
The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression
Authors: Jia Jianping , Chen Zhitao , Li Qingqing , Li Feifei , Liu Siru , Bao Guoliang
Published: April 2024

Astaxanthin has multiple physiological functions and is applied widely. The yeast Phaffia rhodozyma is an ideal source of microbial astaxanthin. However, the stress conditions beneficial for astaxanthin synthesis often inhibit cell growth, leading to low productivity of astaxanthin in this yeast. In this study, 1 mg/L melatonin (MT) could increase the biomass, astaxanthin content, and yield in P. rhodozyma by 21.9, 93.9, and 139.1%, reaching 6.9 g/L, 0.3 mg/g DCW, and 2.2 mg/L, respectively. An RNA-seq-based transcriptomic analysis showed that MT could disturb the transcriptomic profile of P. rhodozyma cell. Furthermore, differentially expressed gene (DEG) analysis show that the genes induced or inhibited significantly by MT were mainly involved in astaxanthin synthesis, metabolite metabolism, substrate transportation, anti-stress, signal transduction, and transcription factor. A mechanism of MT regulating astaxanthin synthesis was proposed in this study. The mechanism is that MT entering the cell interacts with components of various signaling pathways or directly regulates their transcription levels. The altered signals are then transmitted to the transcription factors, which can regulate the expressions of a series of downstream genes as the DEGs. A zinc finger transcription factor gene (ZFTF), one of the most upregulated DEGs, induced by MT was selected to be overexpressed in P. rhodozyma. It was found that the biomass and astaxanthin synthesis of the transformant were further increased compared with those in MT-treatment condition. Combining MT-treatment and ZFTF overexpression in P. rhodozyma, the biomass, astaxanthin content, and yield were 8.6 g/L, 0.6 mg/g DCW, and 4.8 mg/L and increased by 52.1, 233.3, and 399.7% than those in the WT strain under MT-free condition. In this study, the synthesis and regulation theory of astaxanthin is deepened, and an efficient dual strategy for industrial production of microbial astaxanthin is proposed.
The endophytic microbiome response patterns of Juglans regia to two pathogenic fungi
Authors: Wang Ziye , Xu Lu , Lu Xiaoyue , Wang Ruidong , Han Jie , Yan Aihua
Published: April 2024

The endophytic microbial community reassembles to participate in plant immune balance when the host plants are stressed by pathogens. However, it remains unclear whether this assembly is pathogen-specific and how regulatory pathways are coordinated in multi-pathogens. In order to investigate the effects of infection with Colletotrichum gloeosporioides (Cg treatment) and Fusarium proliferatum (Fp treatment) on walnut leaf endophytic microbiome in their assembly, co-occurrence pattern, and on comprehensive chemical function of the internal environment of leaf, an interaction system of the walnut–pathogenic fungi was constructed using seed embryo tissue culture technology. The study showed differences in the assembly of endophytic microbial communities in walnut trees across three groups (control group, Ck; Cg; Fp) after Cg and Fp treatments. Despite changes in relative abundances, the dominant communities in phyla and genera remained comparable during the infection of the two pathogens. Endophyte fungi were more sensitive to the pathogen challenge than endophyte bacteria. Both promoted the enrichment of beneficial bacteria such as Bacillus and Pseudomonas, changed the modularity of the community, and reduced the stability and complexity of the endophyte community. Pathogenic fungi infection mainly affects the metabolism of porphyrin and chlorophyll, purine metabolism, phenylpropane metabolism, and amino acid metabolism. However, there was no significant difference in the secondary metabolites for the different susceptible plants. By screening endogenous antagonistic bacteria, we further verified that Pseudomonas psychrotolerans and Bacillus subtilis had inhibitory effects on the two pathogenic fungi and participated in the interaction between the leaves and pathogenic fungi. The antibacterial substances may be 1-methylnaphthalene, 1,3-butadiene, 2,3-butanediol, and toluene aldehyde.
The effect of DNA-binding proteins on insertion sequence element transposition upstream of the bgl operon in Escherichia coli
Authors: Kopkowski Peter W. , Zhang Zhongge , Saier Milton H.
Published: April 2024

The bglGFB operon in Escherichia coli K-12 strain BW25113, encoding the proteins necessary for the uptake and metabolism of β-glucosides, is normally not expressed. Insertion of either IS1 or IS5 upstream of the bgl promoter activates expression of the operon only when the cell is starving in the presence of a β-glucoside, drastically increasing transcription and allowing the cell to survive and grow using this carbon source. Details surrounding the exact mechanism and regulation of the IS insertional event remain unclear. In this work, the role of several DNA-binding proteins in how they affect the rate of insertion upstream of bgl are examined via mutation assays and protocols measuring transcription. Both Crp and IHF exert a positive effect on insertional Bgl+ mutations when present, active, and functional in the cell. Our results characterize IHF’s effect in conjunction with other mutations, show that IHF’s effect on IS insertion into bgl also affects other operons, and indicate that it may exert its effect by binding to and altering the DNA conformation of IS1 and IS5 in their native locations, rather than by directly influencing transposase gene expression. In contrast, the cAMP-CRP complex acts directly upon the bgl operon by binding upstream of the promoter, presumably altering local DNA into a conformation that enhances IS insertion.
Chemotherapy-induced tubulopathy: a case report series
Authors: Alamilla-Sanchez Mario , Diaz Garcia Juan Daniel , Yanez Salguero Valeria , Morales Lopez Fleuvier , Ulloa Galvan Victor , Velasco Garcia-Lascurain Francisco , Yama Estrella Benjamin
Published: April 2024

Acquired tubulopathies are frequently underdiagnosed. They can be characterized by the renal loss of specific electrolytes or organic solutes, suggesting the location of dysfunction. These tubulopathies phenotypically can resemble Bartter or Gitelman syndrome). These syndromes are infrequent, they may present salt loss resembling the effect of thiazides (Gitelman) or loop diuretics (Bartter). They are characterized by potentially severe hypokalemia, associated with metabolic alkalosis, secondary hyperaldosteronism, and often hypomagnesemia. Tubular dysfunction has been described as nephrotoxic effects of platinum-based chemotherapy. We present 4 cases with biochemical signs of tubular dysfunction (Bartter-like/Gitelman-like phenotype) related to chemotherapy.
Prototype theory and the importance of literary form for moral imagination
Authors: Zheng Yi
Published: April 2024

Prototype theory, which argues that categories have graded (and thus fuzzy) membership based on prototypes, has been used as cognitive evidence to support moral particularism because if categories (in moral rules) only have fuzzy conceptual boundaries, moral rules are not enough for moral judgment, as specific situations also need to be considered to determine how these fuzzy categories should be understood, which is what moral particularism believes. The importance of literature for ethics, especially for moral imagination, has also been extensively discussed because literature can provide vivid examples for us to imagine different moral dilemmas, the consequences of different moral choices, and the feelings of different people facing different situations. Martha Nussbaum specifically argues that the literary form is the only adequate form to imagine certain complex moral situations. By analyzing concrete literary examples as well as the related ethical discussions and empirical findings, this article argues that, building on Nussbaum’s argument, prototype theory can serve as a cognitive basis for the importance of literary form for moral imagination, because the literary form’s tolerance of ambiguity suits how we ambiguously categorize the world.
Roger Sperry, the maverick brain scientist who was haunted by psyche
Authors: Berlucchi Giovanni , Marzi Carlo Alberto
Published: April 2024

This paper describes the scientific figure of Roger Sperry as a maverick researcher, an original thinker who arrived at definitive notions about the working of the brain mostly by distancing himself from the prevalent views of his peers. After solving the riddle of the functions of the corpus callosum, he won a Nobel prize in physiology or medicine for identifying the different cognitive abilities of the disconnected right and left hemispheres of the human brain. He could have won another Nobel prize for his work on the prenatal formation of behavioral neuronal networks and their growth and development after birth. In the last part of his life, he fought a courageous but inconclusive battle for demonstrating that mental and spiritual factors can direct brain activity and behavior without violating the laws of orthodox neurophysiology. Some nodal points in his scientific career and some sources of inspirations for his thinking are identified and discussed within the historical background of the neurosciences of the twentieth century.
High-resolution magnetic resonance vessel wall imaging provides new insights into Moyamoya disease
Authors: Yang Hui , Huang Guilan , Li Xi , Wu Moxin , Zhou Weixin , Yin Xiaoping , Zhang Manqing , Chen Zhiying
Published: April 2024

Moyamoya disease (MMD) is a rare condition that affects the blood vessels of the central nervous system. This cerebrovascular disease is characterized by progressive narrowing and blockage of the internal carotid, middle cerebral, and anterior cerebral arteries, which results in the formation of a compensatory fragile vascular network. Currently, digital subtraction angiography (DSA) is considered the gold standard in diagnosing MMD. However, this diagnostic technique is invasive and may not be suitable for all patients. Hence, non-invasive imaging methods such as computed tomography angiography (CTA) and magnetic resonance angiography (MRA) are often used. However, these methods may have less reliable diagnostic results. Therefore, High-Resolution Magnetic Resonance Vessel Wall Imaging (HR-VWI) has emerged as the most accurate method for observing and analyzing arterial wall structure. It enhances the resolution of arterial walls and enables quantitative and qualitative analysis of plaque, facilitating the identification of atherosclerotic lesions, vascular entrapment, myofibrillar dysplasia, moyamoya vasculopathy, and other related conditions. Consequently, HR-VWI provides a new and more reliable evaluation criterion for diagnosing vascular lesions in patients with Moyamoya disease.
Clinical significance of small extracellular vesicles in cholangiocarcinoma
Authors: Wang Jianjun , Shi Ruizi , Yin Yuan , Luo Hua , Cao Yuan , Lyu Yun , Luo Huiwen , Zeng Xintao , Wang Decai
Published: April 2024

Cholangiocarcinoma is an aggressive and heterogeneous malignancy originating from the bile duct epithelium. It is associated with poor prognosis and high mortality. The global incidence of cholangiocarcinoma is rising, and there is an urgent need for effective early diagnosis and treatment strategies to reduce the burden of this devastating tumor. Small extracellular vesicles, including exosomes and microparticles, are nanoscale vesicles formed by membranes that are released both normally and pathologically from cells, mediating the intercellular transfer of substances and information. Recent studies have demonstrated the involvement of small extracellular vesicles in numerous biological processes, as well as the proliferation, invasion, and metastasis of tumor cells. The present review summarizes the tumorigenic roles of small extracellular vesicles in the cholangiocarcinoma microenvironment. Owing to their unique composition, accessibility, and stability in biological fluids, small extracellular vesicles have emerged as ideal biomarkers for use in liquid biopsies for diagnosing and outcome prediction of cholangiocarcinoma. Specific tissue tropism, theoretical biocompatibility, low clearance, and strong biological barrier penetration of small extracellular vesicles make them suitable drug carriers for cancer therapy. Furthermore, the potential value of small extracellular vesicle-based therapies for cholangiocarcinoma is also reviewed.
Comparison of cytomorphology and histomorphology in myelodysplastic syndromes
Authors: Nachtkamp Kathrin , Strupp Corinna , Faoro Rosa , Gattermann Norbert , Dietrich Sascha , Germing Ulrich , Baldus Stefan
Published: April 2024

Gold standard for the establishment of the diagnosis of myelodysplastic syndromes (MDS) are cytomorphological features of hematopoietic cells in peripheral blood and bone marrow aspirates. There is increasing evidence that bone marrow histomorphology not only aids in the diagnosis of MDS but can provide additional prognostic information, particularly through assessment of fibrosis and cellularity. However, there is only sparse data on direct comparison between histological and cytomorphological findings within the same MDS patient cohort. Therefore, we performed such an analysis under exceptionally well-standardized conditions. We reexamined biopsy material of 128 patients from the Düsseldorf MDS registry who underwent bone marrow trephine biopsy (in addition to bone marrow aspiration) at the time of diagnosis, addressing the following items: a. Analysis of concordance of diagnoses made by histology and cytomorphology b. Analysis of additional information by histology with regard to the diagnosis and prognosis. The respective biomaterials were available at our institution and had been processed according to unchanged protocols between 1992 and 2010. Fresh histopathological sections were obtained from the tissue blocks, stained under identical conditions and re-assessed by a designated expert pathologist (C.B.) without knowledge of the previous histopathological report or the respective cytomorphological diagnosis. The latter, likewise, was uniformly made by the same expert cytomorphologist (U.G.). Histopathology of bone marrow trephine biopsies reliably captured the diagnosis of MDS. Assignment to the diagnostic WHO subgroup was not entirely concordant with cytomorphology, mainly due to incongruences between the proportion of CD34-positive cells on histopathology and the cytomorphological blast count. Histopathology provided additional diagnostic and prognostic information with high diagnostic and prognostic significance, such as fibrosis. Likewise, histopathology allowed more reliable estimation of bone marrow cellularity.
Case report: Rare case of a preoperatively diagnosed spermatic cord paraganglioma and literature review
Authors: Hao Yining , Li Xiuci , Xie Jing , He Wei , Wang Chenghe , Sun Fukang
Published: April 2024

Paraganglioma (PGL) is rare, and PGL that arises from the urogenital system is even rarer. Here we report a case of PGL in spermatic cord and review the relevant literatures. We encountered a 15-year-old boy with a history of hypertension for almost 2 years, accompanied with headache and palpitations. His serum and urine catecholamines were elevated, but no adrenal lesions were detected, suggesting the existence of PGL. Upon physical examination, a painless nodule adherent to the spermatic cord in the right scrotum was found. A systemic Ga68 DOTATATE PET-CT was then performed, and it revealed a mass with high DOTATATE uptake in the right scrotum. The CT, MRI, and ultrasound images showed the abundant blood supply to the tumor. Based on the above-mentioned imaging and biochemical information, a diagnosis of PGL was made prior to surgery. After 2 weeks of preparation with Cardura, an open surgery was performed to remove the tumor together with the right testis and right epididymis. The blood pressure increased to 180/100 mmHg when the tumor was touched intraoperatively and decreased to 90/55 mmHg after the tumor was removed. Post-operative pathology confirmed our diagnosis of PGL originating from the spermatic cord. Immunohistochemical (IHC) staining showed SDHB (+), CgA (+), synaptophysin (+), GATA3 (+), CD56 (+), sertoli cells S-100 (+), and Ki67 (5%). Genetic testing revealed a missense mutation in the SDHA gene. Only 16 cases of spermatic cord PGL have been reported to date. Although it is easy to diagnose by histology and IHC examinations, preoperative diagnosis is quite important as it can actually reduce intraoperative complications.