bluuebunny/crossref_metadata_2025 huggingface dataset:
Eine Konferenz zur sozialen Frage in Basel 1869
Authors: Pernet Martin
Published: September 2024

Basle 1869. The growing industrial proletariat starts to mobilise against bad social conditions. Politically and religiously conservative factory owners hold a conference with town missionaries and clergy to seek ways of alleviating the social malaise. They urge a Christianisation of the industry based on a theological conception that emphasises the experience of individual grace. However, they fail to see the social roots of the human misery. As a result, despite all good intentions, no suitable solution can be found for the various problems.
Epistolae Petri Vireti, ed. Michael W. Bruening, 2012
Authors: Wildt Kees de
Published: September 2024

No abstract available.
118. Jahresbericht des Zwinglivereins über das Jahr 2014
Authors: Rüsch Martin , Bächtold Hans Ulrich
Published: September 2024

No abstract available.
"Veritas evangelica per typographiam": Zur Genese der in Zürich gedruckten Berner Disputationsakten 1528
Authors: Lavater-Briner Hans Rudolf
Published: September 2024

The Bern Disputation of 1528 was both the response to the Baden Disputation of 1526 and its continuation. From a formal perspective, the Baden served as a model for the Bernese. However, with respect to the printing of the disputation acts, this model needed to be improved upon as the delayed and flawed Lucerne edition of the Baden acts had led to justified doubts about the reliability of its protocol. In Bern, the four parallel transcripts of their disputation written under the direction of city secretary Peter Cyro were first collated before being delivered to the printer Christoph Froschauer in Zurich by one of the notaries charged with supervising the production of the complete edition. As a result of close cooperation between the typesetter and two theologically educated proofreaders, and under the eyes of Zwingli, a quarto edition appeared on March 23, 1528, almost two months after the Bern disputation and in time for the Frankfurt Book Fair. It was followed exactly one month later by an octavo edition.
Joachim Vadians Konzilsgedicht "Freu dich, Hierusalem": Eine Entgegnung auf die päpstliche Einladung zum Konzil von Trient
Authors: Gamper Rudolf
Published: September 2024

The mayor of St Gallen, Joachim Vadian (1484–1551), composed numerous poems in the 1540s on diverse subjects. In a recently discovered poem, he confronts the invitation of the pope to the council of Trient with the views of the Reformed church. This paper edits and translates the poem, and places it in the context of Vadian’s known works on the council.
Reconceiving the Clerical Corps: How Heinrich Bullinger Resists the Expectations of Confessionalization
Authors: Wood Jon Delmas
Published: September 2024

The traditional concept of priesthood aroused much interest and animosity in the Reformation. Huldrych Zwingli led efforts to expand priesthood beyond a sacramental subset of Christendom to encompass instead the sum of all the baptized. He redefined clerical identity as a humanistic/linguistic corps of “prophets.” Heinrich Bullinger shared in Zwingli’s mission to preserve a ministerial corps, and yet he found it increasingly necessary to modify the mold of “minister-as-prophet.” Bullinger carefully re-appropriated some aspects of a “priestly” identity for the clerical corps. In the process, he created a fruitful – perhaps even unique – hybrid of humanism with eschatology. The upshot was a model for church-state relations that cannot be reduced to the expectations of propositional dogmatism commonly associated with the theory of confessionalization.
Marion Dammaschke und Günter Vogler, Thomas Müntzer Bibliographie, 2013
Authors: Leu Urs B.
Published: September 2024

No abstract available.
Leo Jud: The Shorter Catechism
Authors: Priestley David T.
Published: September 2024

English translation of Leo Jud’s "Kuertzer Catechismus" (1538) with biographical sketch.
Neue Literatur zur zwinglischen Reformation
Authors: Bächtold Hans Ulrich
Published: September 2024

No abstract available.
120. Jahresbericht des Zwinglivereins über das Jahr 2016
Authors: Rüsch Martin , Bächtold Hans Ulrich
Published: September 2024

No abstract available.
114. Jahresbericht des Zwinglivereins über das Jahr 2010
Authors: Senn Matthias
Published: September 2024

No abstract available.
Wilhelm Reublin als Kopist: Die Abschrift des Arzneibuchs des Johann Poll, Znaim 1549
Authors: Rothkegel Martin
Published: September 2024

Wilhelm Reublin, a pioneer of Zurich Anabaptism who played an important role in Southwestern Germany and Moravia, lived from 1535 until his death (ca. 1559) in the South Moravian town of Znojmo after his retreat from anabaptist congregational life. To support himself, he took on occasional jobs, among others work as a copyist of Latin texts. It is highly likely that part of the manuscript Pharmacopoeia, cod. 11410 in the Austrian National Library in Vienna, is in his hand.
Die Zuger haben das Wort Gottes verbrannt - Strategien der konfessionellen Polemik am Beispiel einer reformatorischen Schmähschrift vom Jahr 1556
Authors: Göttler Christine
Published: September 2024

no abstract available.
Emidio Campi, Shifting Patterns of Reformed Tradition, 2014
Authors: West Jim
Published: September 2024

No abstract available.
Zur Führungsrolle des Grauen Bundes und der Stadt Ilanz in der frühen Reformbewegung Graubündens
Authors: Bundi Martin
Published: September 2024

Of the seven crucial reform decrees written between 1523 and 1526 in the three free states of Graubunden, five were issued in Ilanz, and two in Chur. Ilanz, the first and only Romansch speaking city on the Rhine was the center of the Grey League and fostered a close relationship to Zwingli’s Zurich as well as with southern Germany. A school of writing developed in this location, whilst the chancery of the Grey League maintained from here diplomatic correspondences throughout Europe. The scholarly clerics and politicians trained in humanism prepared the official decrees that led to the breakthrough of the Reformation.
Le réformateur Jean Le Comte (1500–1572): De l’oubli à une mémoire remodelée
Authors: Bodenmann Reinhard
Published: September 2024

In a publication of 1707, Abraham Ruchat (1680–1750) exhumes for the first time the reformer Jean Le Comte (1500–1572), hitherto completely unknown to Protestant historiography. According to Ruchat, Le Comte was, along with Guillaume Farel and Pierre Viret, one of the most important reformers of the Romandie. To back his assertion, he refers to a diary supposed to have been written by Le Comte himself and derives from it new details about the Reformation, pertaining especially to the dominion of Grandson. Since then, historians have used the extracts excerpted by Ruchat over and over again, while a few others claim to have used the diary itself, the last of which was Eduard Bähler at the end of the 19th century. It was assumed since then that this diary of Jean Le Comte had been lost. This article establishes that the diary was not written by Jean Le Comte, but by his son Jacques (1543–1613) on the basis of paternal archives, afterwards destroyed by the son in order to blur any evidence of the strong disagreements which existed between his father on the one hand and the reformers Farel, Calvin and Viret on the other. This original diary composed by Jacques, which still existed in 1767, has probably been destroyed since. However, extracts of it dating from 1629 have been rediscovered, similar and yet a little bit more exhaustive than the ones known so far. They were discovered for the first time at the end of 1916 in the castle of Champvent, were shown at that time to the well-known historian of the Canton Vaud, Henri Vuilleumier (1841–1925), before falling into oblivion once again.
Ulrich Pfister, Konfessionskirchen, Glaubenspraxis und Konflikt in Graubünden, 2012
Authors: Head Randolph C.
Published: September 2024

No abstract available.
Buchdruck und Reformation in Genf (1478-1600): Ein Überblick
Authors: Würgler Andreas
Published: September 2024

This article links profits from the rich literature in the fields of Reformation history and book-history in order to analyse the relation between printing and the Reformation in Geneva from 1478 to 1600 in a general historian’s perspective. Although various genres of religious and literary (illustrated) books have been printed for a local market since 1478, texts with evangelical  tendency have not been produced in Geneva before 1536. Therefore, the ideas of Reformation were rather introduced by evangelical preachers than by the printing press. But after that Geneva became the centre of protestant or better: Calvinist printed propaganda in French (and Latin) until the early 1560s. Because of changing political contexts – French wars of Religion and the comeback of the Duke of Savoy as a neighbour – and the emerging of reformed printing in France forced Geneva to redirect their production towards more learned and non-religious books in Latin (humanism, classical authors, science). Nether the less, the production of (Genevan) Bibles and texts of Calvin and his followers and successors did not cease.
Vom Karpatenbecken zum Rheinknie: Eine Quellenanalyse zu den Beziehungen der ungarländischen reformierten Kollegien in Debrecen und Sárospatak zur Universität Basel im 18. Jahrhundert
Authors: Hegyi Ádám
Published: September 2024

Among the countries belonging to the Holy Crown of Saint Stephen in the 18th century, Hungary and Transylvania were governed as separate administrative units because Habsburg rulers did not unite the original state formation of integer Hungary that had been disintegrated in the 16th century. Therefore, the protestant churches had to cope with different situations in the Habsburg monarchy, among which this study focuses only on the Hungarian situation. In the Age of Enlightenment the Reformed Church had two centres of education in Hungary (Debrecen and Sárospatak), which achieved the college level, but did not reach the level of a university. Consequently, Western European universities had a highly significant role in the education of Calvinist intellectuals, as the Hungarian students could attain a university level education only at these institutions. The University of Basel was particularly important for Hungarian students in the 18th century because one third of the overall number of students consisted of only Hungarians at certain periods. Moreover, one could even observe a moderate competition among students arriving in Switzerland from the Debrecen and Sárospatak Colleges respectively, as the professors of both institutions lobbied for scholarships at Basel. This study pre-eminently investigates this process, while also examining the Hungarian relations of book publications in Basel.
Ecología integral: reflexiones de un converso
Authors: Guerra Ángel
Published: September 2024

Partiendo de una actitud de sospecha, en este artículo se aportan argumentos para admitir que la ecología integral es un nuevo enfoque ecológico primordial en servicio del bien común de la humanidad. Se analizapor qué este nuevo concepto ecológico, que incorpora el lugar peculiar del ser humano en la naturaleza y sus relaciones con la realidad que lo rodea, requiere de una antropología correcta. Se encuentra esta antropología en el personalismo integral y el cristianismo, donde el significado de la sexualidad humana se considera con todas sus consecuencias. Se subraya que esta concepción de la ecología propone acciones concretas que afectan al comportamiento, prioridades, compromisos y sensibilidad de cada persona humana, y que genera la obligación ética de dejar un mundo sostenible a esta y a las próximas generaciones. Procedentes de la ecología sensu stricto, se recogen varios conceptos que ayudan a definir la episteme de la ecología integral. Estas nociones son: la capacidad de carga de un ecosistema, el principio de san Mateo, las ideas de energía exosomática y de artefactos exosomáticos y el principio de precaución. Se rechazan como antihumanas las premisas ideológicas del ecocentrismo, el biocentrismo y el modelo interpretativo de Gaia
Technology Cannot a Revolution Make: Nas-book not Facebook
Authors: Dajani Nabil
Published: September 2024

“What happened in Tunisia was not a Facebook revolution, and what’s happening now in Egypt is not a potential Twitter revolution...technology cannot a revolution make.” --Nicholas Thompson (“Is Twitter Helping in Egypt?”) in the New Yorker, January 27, 2011 Examining the ongoing academic debate over the effect of the new social media on social movements, I get the impression that we are returning to the era of the “magic bullet” theory that was popular at the beginnings of the past century when people were fascinated by the power of the then-new media outlets of radio and film. The belief then was that the effect of these new platforms was massive and uniform. The present euphoria about social media suggests that we are going through another cycle of theories about media effects, returning to the “magic bullet” argument.
“Tirar del Hilito”
Authors: Aravena Aravena Cristian
Published: September 2024

Este artículo da cuenta de cómo la escritura de la dramaturga chilena Isidora Aguirre transita entre el melodrama social, pasando por un teatro épico, para llegar al teatro político y documental. Acá se muestran cómo las estrategias de lo documental fueron tomándose sus creaciones, tímidamente con la obra Población Esperanza de 1959, escrita junto a Manuel Rojas, pero sobre todo con Los papeleros de 1961 y Los que van quedando en el camino de 1969.
Pengembangan Bahan Ajar Menarik dengan Bantuan Kecerdasan Buatan
Authors: Budiman Adam Arif , Setiyaningsih Timor , Astuti Eka Yuni
Published: September 2024

Pengajaran di sekolah dasar memerlukan pendekatan yang memperhatikan karakteristik siswa, termasuk tingkat perkembangan kognitif dan minat belajar mereka. Materi ajar yang menarik dan bersifat visual dapat meningkatkan motivasi siswa serta memperkuat pemahaman. Namun, tidak semua guru memiliki kemampuan untuk membuat bahan ajar yang kreatif dan interaktif. Beberapa kendala yang dihadapi oleh guru dalam mengembangkan bahan ajar meliputi keterbatasan kreativitas, kurangnya keterampilan teknologi, serta kecenderungan mengandalkan sumber daya yang sudah ada. Menggabungkan konten menarik dengan fitur interaktif juga memerlukan pemahaman mendalam tentang materi pelajaran dan cara penyajiannya.dengan Pelatihan pengembangan bahan Ajar berbasis tool kecerdasan Buatan membuat guru mudah membuat bahan ajar yang menarik dan cepat
Læringspotentialet i (længere) vandreture med elever i historie
Authors: Pietras Jens
Published: September 2024

Kravet om 45 min. bevægelse i fagene kom formelt ind i skolen med skolereformen 2013 som en kompensation og modvægt til de dengang indførte såkaldte lange skoledage. I regeringens nyeste folkeskoleaftale fra marts 2024 er faglig bevægelse blevet frivilligt. I denne artikel argumenteres for fortsat brug af faglig bevægelse i historie – særligt i forbindelse med læringspotentialet gennem længere vandringer med eleverne.
Les disparitions forcées dans le monde contemporain : les contributions récentes du Groupe de travail des Nations Unies sur les disparitions forcées ou involontaires
Authors: Duhaime Bernard
Published: September 2024

Bien que les disparitions forcées soient souvent associées aux anciens régimes répressifs latino-américains, elles se produisent encore aujourd’hui, et ce, dans le monde entier et dans divers contextes. En plus de servir de stratégie d’oppression politique, elles sont utilisées dans le contexte de conflits armés, dans le cadre de mesures antiterroristes, lors de transferts transnationaux, ou encore dans un contexte migratoire. Dans cet article, il est proposé de passer en revue les conceptions traditionnelles concernant cette violation odieuse des droits de la personne et de traiter de ce que certaines personnes appellent les nouvelles formes de disparitions forcées. L’auteur se questionne notamment sur les caractéristiques communes des disparitions forcées du passé et du présent, ainsi que sur la façon dont cela devrait nourrir les débats contemporains sur le sujet. Il aborde aussi les apports du Groupe de travail des Nations Unies sur les disparitions forcées ainsi que les obligations étatiques en la matière. Il en ressort que l’interdiction de ce crime est une norme de jus cogens et que les États ont une obligation erga omnes de le prévenir et de le sanctionner, sans limite spatiale ou temporelle. Il conclut avec un appel à la communauté internationale à lutter contre l’impunité et à soutenir les victimes de disparitions forcées et leurs proches.
Microbial diversity of biofilm in spent nuclear fuel storage pond
Authors: Rahayu Dyah Sulistyani , Ambarsari Laksmi , Nurcholis Waras , Shalsabilla Syalwa Ersadiwi , Fadilah Alma , Sanjaya Muhammad Fajar , Sambari Rahmad Ramdani , Ramadhan Firdaus , Rijal Mohammad Syamsul , Nadyaputri Intan Ghaisani , Sugoro Irawan
Published: September 2024

Spent nuclear fuel (SNF) storage is a facility requiring careful attention to prevent corrosion, with microbial-induced corrosion (MIC) being a significant contributing factor. While previous investigations have genetically identified total microbes and sulfate-reducing bacteria (SRB) in SNF storage ponds, similar studies have highlighted the dominance of Proteobacteria and Firmicutes in these environments. Therefore, this study aimed to identify the diversity of bacteria biofilm in SNF storage ponds through a metagenomic approach, with a specific focus on those potentially causing MIC. The results showed that the rack had the highest number of taxa based on taxonomic identification. The bacteria community on the rack, at the phylum level, was dominated by Proteobacteria (34.04%), Firmicutes (24.96%), and Chloroflexi (20.52%). Chloroflexi constituted the majority of the bacteria community on the pool floor (91.09%), while on the pool walls, Chloroflexi (45.17%) and Proteobacteria (44.02%) dominated almost equally. Metabolic pathway analysis further confirmed the activity of MIC in biofilm by the presence of MIC-related pathways. These findings contribute novel insights into the microbial composition of biofilms in SNF storage ponds, providing a foundation for future studies on the prevention and management of MIC in such facilities.
Cell crowding induces TRPV4 inhibition and its relocation to plasma membranes, implicating pro-invasive cell volume reduction mechanotransduction pathway
Authors: Bu Xiangning , Ashby Nathanael , Vitali Teresa , Lee Sulgi , Gottumukkala Ananya , Yun Kangsun , Tabbara Sana , Latham Patricia , Teal Christine , Chung Inhee
Published: September 2024

Cell crowding is a common microenvironmental factor that affects various disease processes, but its impact on cell invasiveness into surrounding tissues is not well understood. This study investigates the biomechanical changes induced by cell crowding, focusing on pro-invasive cell volume reduction in ductal carcinoma in situ (DCIS) cells. DCIS is a non-invasive form of breast cancer characterized by abnormal cell growth confined within the breast duct. While DCIS can undergo invasive transition, it is unclear which DCIS cells are predisposed to this transition. We discovered that cell crowding enhanced the invasiveness of high-grade DCIS cells, which experienced significant cell volume reduction compared to hyperplasia-mimicking or normal cells. Mass spectrometry analyses revealed that cell crowding relocated ion channels, including TRPV4, a calcium-permeant ion channel, to the plasma membrane selectively in high-grade DCIS cells but not in less aggressive or normal cells. Cell crowding inhibited TRPV4 activity in high-grade DCIS cells, decreasing intracellular calcium levels and reducing cell volume. This inhibition also triggered the relocation of TRPV4 to the plasma membrane, effectively priming the inactive channel for activation and mitigating the calcium loss caused by crowding-induced inhibition. Analyses of patient-derived breast cancer tissues validated that TRPV4 is selectively associated with the plasma membrane in high-grade DCIS but not in lower-grade DCIS or less aggressive pathologies. The extent of plasma membrane TRPV4 association scaled with cell volume reduction and increased cell invasiveness and motility, suggesting its utility as an active pro-invasive mechanotransduction pathway indicator. Additionally, hyperosmotic conditions and pharmacologic TRPV4 inhibition mimicked the pro-invasive volume reduction observed under cell crowding, while TRPV4 activation reversed this effect by inducing cell volume increase. Silencing the TRPV4 gene via shRNA diminished the mechanotransduction capability of high-grade DCIS cells, as demonstrated by reduced intracellular calcium depletion, attenuated cell volume reduction, and decreased motility. In summary, this study uncovers a previously unrecognized pro-invasive mechanotransduction pathway initiated by cell crowding, which is specific to high-grade DCIS cells, revealing a potential biomarker for identifying DCIS patients at high risk of invasive transition.
Parallel reconstruction of the excitatory and inhibitory inputs received by single neurons reveals the synaptic basis of recurrent spiking
Authors: Bartram Julian , Franke Felix , Kumar Sreedhar Saseendran , Buccino Alessio Paolo , Xue Xiaohan , Gänswein Tobias , Schröter Manuel , Kim Taehoon , Kasuba Krishna Chaitanya , Hierlemann Andreas
Published: September 2024

Self-sustained recurrent activity in cortical networks is thought to be important for multiple crucial processes, including circuit development and homeostasis. Yet, the precise relationship between synaptic input patterns and the spiking output of individual neurons remains largely unresolved. Here, we developed, validated and applied a novel in vitro experimental platform and analytical procedures that provide – for individual neurons – simultaneous excitatory and inhibitory synaptic activity estimates during recurrent network activity. Our approach combines whole-network high-density microelectrode array (HD-MEA) recordings from rat neuronal cultures with patch clamping and enables a comprehensive mapping and characterization of active incoming connections to single postsynaptic neurons. We found that, during network states with excitation(E)-inhibition(I) balance, postsynaptic spiking often coincided with the maxima of fast fluctuations in the input E/I ratio. These spike-associated E/I ratio escalations were largely due to a rapid bidirectional change in synaptic inhibition that was modulated by the network-activity level. Our approach also uncovered the underlying circuit architecture and we show that individual neurons received a few key inhibitory connections – often from special hub neurons – that were instrumental in controlling postsynaptic spiking. Balanced network theory predicts dynamical regimes governed by small and rapid input fluctuation and featuring a fast neuronal responsiveness. Our findings – obtained in self-organized neuronal cultures – suggest that the emergence of these favorable regimes and associated network architectures is an inherent property of cortical networks in general.
Exceptional longevity of mammalian ovarian and oocyte macromolecules throughout the reproductive lifespan
Authors: Bomba-Warczak Ewa K , Velez Karen M , Zhou Luhan T , Guillermier Christelle , Edassery Seby , Steinhauser Matthew L , Savas Jeffrey N , Duncan Francesca E
Published: September 2024

The mechanisms contributing to age-related deterioration of the female reproductive system are complex, however aberrant protein homeostasis is a major contributor. We elucidated exceptionally stable proteins, structures, and macromolecules that persist in mammalian ovaries and gametes across the reproductive lifespan. Ovaries exhibit localized structural and cell-type specific enrichment of stable macromolecules in both the follicular and extrafollicular environments. Moreover, ovaries and oocytes both harbor a panel of exceptionally long-lived proteins, including cytoskeletal, mitochondrial, and oocyte-derived proteins. The exceptional persistence of these long-lived molecules suggest a critical role in lifelong maintenance and age-dependent deterioration of reproductive tissues.
Confirmation of HLA-II associations with TB susceptibility in admixed African samples
Authors: Croock Dayna , Swart Yolandi , Schurz Haiko , Petersen Desiree C , Möller Marlo , Uren Caitlin
Published: September 2024

The International Tuberculosis Host Genetics Consortium (ITHGC) demonstrated the power of large-scale GWAS analysis across diverse ancestries in identifying tuberculosis (TB) susceptibility loci. Despite identifying a significant genetic correlate in the human leukocyte antigen (HLA)-II region, this association did not replicate in the African ancestry-specific analysis, due to small sample size and the inclusion of admixed samples. Our study aimed to build upon the findings from the ITHGC and identify TB susceptibility loci in an admixed South African cohort using the local ancestry allelic adjusted association (LAAA) model. We identified a near-genome-wide significant association (
rs3117230
,
p
-value = 5.292 x10
-6
, OR = 0.437, SE = 0.182) in the
HLA-DPB1
gene originating from KhoeSan ancestry. These findings extend the work of the ITHGC, underscore the need for innovative strategies in studying complex admixed populations, and confirm the role of the HLA-II region in TB susceptibility in admixed South African samples.
Confirmation of HLA-II associations with TB susceptibility in admixed African samples
Authors: Croock Dayna , Swart Yolandi , Schurz Haiko , Petersen Desiree C , Möller Marlo , Uren Caitlin
Published: September 2024

The International Tuberculosis Host Genetics Consortium (ITHGC) demonstrated the power of large-scale GWAS analysis across diverse ancestries in identifying tuberculosis (TB) susceptibility loci. Despite identifying a significant genetic correlate in the human leukocyte antigen (HLA)-II region, this association did not replicate in the African ancestry-specific analysis, due to small sample size and the inclusion of admixed samples. Our study aimed to build upon the findings from the ITHGC and identify TB susceptibility loci in an admixed South African cohort using the local ancestry allelic adjusted association (LAAA) model. We identified a near-genome-wide significant association (
rs3117230
,
p
-value = 5.292 ×10
−6
, OR = 0.437, SE = 0.182) in the
HLA-DPB1
gene originating from KhoeSan ancestry. These findings extend the work of the ITHGC, underscore the need for innovative strategies in studying complex admixed populations, and confirm the role of the HLA-II region in TB susceptibility in admixed South African samples. [148/150 words]
Executioner caspase is proximal to Fasciclin 3 which facilitates non-lethal activation in Drosophila olfactory receptor neurons
Authors: Muramoto Masaya , Hanawa Nozomi , Okumura Misako , Chihara Takahiro , Miura Masayuki , Shinoda Natsuki
Published: September 2024

The nervous system undergoes functional modification independent on cell turn over. Caspase participates in reversible neuronal modulation via non-lethal activation. However, the mechanism that enables non-lethal activation remains unclear. Here, we analyzed proximal proteins of
Drosophila
executioner caspase in the adult brain using TurboID. We discovered that executioner caspase Drice is, as an inactive proform, proximal to cell membrane proteins, including a specific splicing isoform of cell adhesion molecule Fasciclin 3 (Fas3), Fas3G. To investigate whether sequestration of executioner caspase to plasma membrane of axons is the mechanism for non-lethal activation, we developed a Gal4-Manipulated Area-Specific CaspaseTracker/CasExpress system for sensitive monitoring of caspase activity near plasma membrane. We demonstrated that
Fas3G
-overexpression promotes caspase activation in olfactory receptor neurons without killing them, by inducing expression of initiator caspase Dronc, which also comes close to Fas3G. Physiologically,
Fas3G
-overexpression facilitated non-lethal activation suppresses innate olfactory attraction behavior. Our findings suggest that subcellularly-restricted caspase activation, defined by caspase proximal proteins, is the mechanism for non-lethal activation, opening the methodological development of reversible modification of neuronal function via regulating caspase proximal proteins.
Relaciones públicas internacionales en las empresas multilatinas colombianas
Authors: Giraldo Dávila Andrés
Published: September 2024

El auge de las multilatinas se da por el aprendizaje que las empresas locales tuvieron de la apertura económica y el ‘boom’ de la globalización. La inversión extranjera directa (IED) fue la variable macroeconómica que potenció el surgimiento de las empresas transnacionales y no fue algo exclusivo de las economías desarrolladas sino que muchos de estos actores tienen como origen mercados emergentes, logrando así equilibrar el terreno de juego económico en un diálogo sur-sur. A través de un análisis Delphi con 20 expertos – entre profesionales y académicos de las relaciones públicas en América Latina- y una encuesta estructurada a 24 directores de comunicación (dircoms) de las multilatinas colombianas, se trazaron líneas descriptivas en torno al papel que juegan los procesos de comunicación en la expansión de las multinacionales de Colombia. La identificación de los intereses de los stakeholders internacionales y la conexión estratégica entre casa matriz y filiales constituyen los ejes centrales para el adecuado posicionamiento de las organizaciones colombianas en el exterior. Las multilatinas de es esta parte del continente consideran que, una buena gestión de la reputación corporativa es la que ayuda al posicionamiento de las empresas en los distintos países, conectando la planificación de las relaciones públicas desde la matriz y considerando los intereses específicos de cada subsidiaria para su ejecución, entendiendo que los contextos de cada país permiten ajustar la estrategia macro de la organización.
Omnichannel diagnosis for micro, small and medium-sized companies, sectorial study
Authors: Valencia Cardenas Marisol , Restrepo Morales Jorge Anibal , López Cadavid Diego , Vanegas López Juan Gabriel
Published: September 2024

Este estudio explica las características y comportamientos omnicanal de las micro, pequeñas y medianas empresas (MIPYMES) del sector alimentario en Antioquia, Colombia. Evalúa las condiciones necesarias para alcanzar una clasificación omnicanal basada en tres dimensiones: modelos de marketing, opciones multicanal y cadena de suministro con tecnologías digitales. Se empleó una metodología explicativa utilizando datos empíricos de encuestas administradas a 140 MIPYMES, y se utilizó el análisis de conglomerados para agrupar a las empresas. Los resultados muestran un comportamiento omnicanal general relativamente bajo, con las empresas medianas superando a las pequeñas y a las microempresas. Las tecnologías digitales mejoradas y las plataformas integradas pueden mejorar significativamente las condiciones omnicanal, lo que conduce a una mejor rentabilidad y retención de clientes. Los hallazgos enfatizan la importancia de la integración digital y la necesidad de más investigación sobre los aspectos tecnológicos y metodológicos de la implementación omnicanal.
Diversidad de la identidad hispana en la serie de ficción bilingüe Now and Then de Apple TV Plus
Authors: Santana Mahmut Saida , Sanz de León Vicente , Montes Rodríguez Gustavo
Published: September 2024

La ficción audiovisual puede reforzar estereotipos sociales, pero puede también derribarlos mostrando personajes cuyas acciones son normalmente encasilladas en otros comportamientos. Este estudio busca conocer las características de representación de la diversidad de la identidad hispana de la serie de ficción Now and Then, primera serie española de Apple TV que busca romper con los estereotipos negativos habituales de los latinos en la ficción internacional. Para ello, se utiliza el análisis narrativo, centrado en las características de los personajes principales y la entrevista en profundidad a sus creadores. Se constata que Now and Then se diferencia de las series que uniformizan al hispano como estereotipo de clase baja con acento único neutralizado y que representa la multiculturalidad y diversidad hispana en roles de distintas nacionalidades que conservan su lengua y acento, reflejan su lugar de origen e idiosincrasia y otro estatus económico.
Análise decisória para implementação de estratégias integradas para a redução de setup em processos industriais utilizando lógica fuzzy
Authors: Souza Vanessa Silva , Alencar David Barbosa de , Silva Ítalo Rodrigo Soares
Published: September 2024

Este artigo explora a aplicação da Lógica Fuzzy como ferramenta de suporte na tomada de decisão para implementar estratégias integradas com o objetivo de reduzir tempos de setup em processos industriais. O estudo visa otimizar a eficiência operacional e diminuir a variabilidade do processo produtivo, o que, em última análise, eleva a produtividade. Desenvolveu-se um modelo computacional baseado em Lógica Fuzzy para analisar variáveis principais que afetam o tempo de setup e o desperdício associado, que resulta em baixa produtividade. A pesquisa foi realizada em uma empresa do Polo Industrial de Manaus, no setor eletrônico, com os seguintes objetivos específicos: analisar detalhadamente o processo de setup; identificar causas e efeitos principais através da análise de variáveis linguísticas; implementar um modelo de decisão baseado em Lógica Fuzzy; e avaliar a eficácia desse modelo na redução do tempo de setup. A metodologia exploratória adotada consistiu em três etapas: Especificação de Requisitos, para definir parâmetros e critérios do modelo; Processo de Simulação, onde o modelo foi testado e ajustado em ambiente controlado; e Análise dos Resultados, para avaliar a performance do modelo com base nos dados coletados. Os resultados mostraram que a combinação das variáveis Ferramentas e Equipamentos com Planejamento Antecipado tem um impacto significativo de 86% sobre o tempo de setup. Este achado destaca a importância da gestão eficaz dessas variáveis para reduzir gargalos e desperdícios de tempo, melhorando assim a produtividade global da linha de produção. O modelo desenvolvido provou ser uma ferramenta promissora para identificar e mitigar fatores que afetam negativamente a eficiência operacional.
Yield Gap Analysis and Impact of Demonstration on Turmeric in Tirap District of Arunachal Pradesh, India
Authors: Chaturvedi Abhimanyu , Singh Ph. Chandramani , Bagra Gerik
Published: September 2024

The north eastern region of India having good area under spices viz. turmeric, ginger etc. But the productivity is too low due to adoption of old varieties, as well as non-recommended practices followed by farmers. In this context, Megha Turmeric-1 variety of turmeric was demonstrated under front line demonstration programme during 2020-21 and 2021-22 respectively by Krishi Vigyan Kendra Tirap, Arunachal Pradesh. The average FLD yield was 218 and 224 q/ha was recorded as compared to 164 and 179 q/ha under farmers practices. The Technology gap was 32 and 26 q/ha, extension gap was 54 and 45 q/ha and technology index ere 13 and 10 respectively. The net income under FLD plot was higher over farmers practice during both years of study (Rs, 290000 & 300000 vs. 210000 & 214000). Similarly, the B:C ratio was also better than farmers practice (2.45 & 2.02 vs. 1.77 & 1.76). Being a very low technology index, it assumes that turmeric production can be enhanced by dissemination of improved technologies at farmer’s field.
Eco-Friendly Beverage Innovation: Utilizing Orange and Pineapple Byproducts for Nutrient-Rich Tea Blends
Authors: Emmanuel Chi Ngunjoh , Kukwa Rose Erdoo , Leke Luter
Published: September 2024

Aims: production and analysis of tea from orange rind and pineapple core, focusing on the physicochemical, phytochemical, antioxidant, and sensory properties of the tea blends
Study Design: Five variations of orange rind and pineapple core powder in 100:0, 75:25, 50:50, 25:75, and 0:100 ratios were the experimental lots, labelled A to E, respectively, and a commercial green tea labelled sample F was used as a control.
Place and Duration of Study: oranges and pineapple fruit were bought from the railway market in Makurdi, Benue state of Nigeria. a standard commercial green tea was purchased from a supermarket in Makurdi Benue State of Nigeria.
Methodology: Various analytical techniques were employed to assess the tea's physicochemical properties, anti-nutrient, phytochemical content and antioxidant capacity. Sensory evaluations were also conducted to gauge consumer acceptance and preference.
Results: The physicochemical properties of the tea ranged from pH (4.7-5.6), TTA (0.62-1.00%), TSS (8.45-10.47 °Brix), Brix/acidity ratio (6.27-13.69). The anti-nutrient content ranged as follows: alkaloids (0.083-0.113%), oxalates (0.032-0.048%), phytates (0.001-0.003%), and tannins (0.0005-0.0124%). The phytochemical content was as follows: total flavonoids (5.45-7.61 mgQE/g), total phenols (1.36-3.98 mgGAE/g). The samples also demonstrated significant antioxidant activity as follows; FRAP (2.23-8.79 mgAAE/g), H2O2 scavenging ability (4.23-10.11%). Importantly, sensory results showed that all the herbal tea samples were well-received and accepted by the panellists, indicating their potential in the market.
Conclusion: The tea samples produced demonstrated great potential for its use as a functional beverage and as a substitute for other tea brands, given its improved functional characteristics. This study explored the potential applications of orange and pineapple by-products in sustainable product development.
Analysis of Nutritional Profiles, Mineral Content, and Contaminants in Palm Oil from Yenagoa Metropolis, Nigeria: Assessing Quality and Safety Standards
Authors: Bennett Victoria , Ebisintei Precious
Published: September 2024

Aim: This study aims to evaluate the nutritional profiles, chemical compositions, and potential contamination of palm oil samples from different locations in Yenagoa Metropolis, Nigeria, to assess their quality and safety.
Study Design: An analytical investigation was conducted, using proximate analysis, mineral content determination, and Gas Chromatography-Mass Spectrometry (GC-MS) to characterize the palm oil samples' composition and contamination levels.
Place and Duration of Study: The research was carried out in Yenagoa Metropolis, Nigeria, from January to October 2023.
Methods: Palm oil samples were collected from three distinct locations within Yenagoa Metropolis. Proximate analysis quantified moisture, ash, protein, fat, and carbohydrate contents. Mineral content was assessed for potassium, iron, magnesium, and phosphorus. GC-MS was employed to identify and quantify bioactive compounds and potential contaminants in the samples.
Results: Moisture content ranged from 0.00% in sample C to 3.00% in sample A. Ash content was highest in samples B and C, at 20.06%, while sample C had the highest fat content at 47.0%. Potassium (10.9%) and phosphorus (0.53%) were also highest in sample C. GC-MS analysis revealed key bioactive compounds, such as n-hexadecanoic acid and squalene, but also detected harmful contaminants especially in samples A and B. Presence of Isobutylene glycol and various oxiranes can cause skin, eye, and respiratory irritation, with prolonged exposure leading to severe health issues. Benzene, toluene, Trimethylamine compound with Borane, Pentane 3-ethyl-2-methyl and Heptane 2-methyl are associated with respiratory problems, neurological damage, and potential long-term organ effects. Contaminants in the oil samples were attributed to inadequate refining, contaminated raw materials, improper handling, and environmental pollution. This study emphasizes the nutritional value of palm oil while highlighting the need for improved quality control measures to ensure the safety and efficacy of palm oil products.
Functional identification of AtAVT3, a family of vacuolar amino acid transporters, in Arabidopsis
Authors: Fujiki Yuki , Teshima Hiromitsu , Kashiwao Shinji , Kawano‐Kawada Miyuki , Ohsumi Yoshinori , Kakinuma Yoshimi , Sekito Takayuki
Published: December 2016

Amino acids stored in the vacuoles are exported to the cytosol mainly for protein synthesis; however, the molecular identity of vacuolar amino acid exporters remains obscure in plants. Here, we demonstrate that the heterologous expression of AtAVT3 genes, Arabidopsis homologs of AVT3 and AVT4 encoding vacuolar amino acid exporters in yeast, reduces vacuolar amino acid levels in the avt3∆avt4∆ yeast cells. In vitro experiments revealed that 14C‐labeled Ala and Pro are exported from vacuolar membrane vesicles by AtAvt3A in an ATP‐dependent manner. In Arabidopsis, AtAvt3A fused with green fluorescent protein localizes to the vacuolar membrane. We propose that AtAVT3 family represents the long sought‐for vacuolar amino acid exporters in plants.
Rab28 is a TBC1D1/TBC1D4 substrate involved in GLUT4 trafficking
Authors: Zhou Zhou , Menzel Franziska , Benninghoff Tim , Chadt Alexandra , Du Chen , Holman Geoffrey D. , Al‐Hasani Hadi
Published: December 2016

The Rab‐GTPase‐activating proteins (GAPs) TBC1D1 and TBC1D4 play important roles in the insulin‐stimulated translocation of the glucose transporter GLUT4 from intracellular vesicles to the plasma membrane in muscle cells and adipocytes. We identified Rab28 as a substrate for the GAP domains of both TBC1D1 and TBC1D4 in vitro. Rab28 is expressed in adipose cells and skeletal muscle, and its GTP‐binding state is acutely regulated by insulin. We found that in intact isolated mouse skeletal muscle, siRNA‐mediated knockdown of Rab28 decreases basal glucose uptake. Conversely, in primary rat adipose cells, overexpression of Rab28‐Q72L, a constitutively active mutant, increases basal cell surface levels of an epitope‐tagged HA‐GLUT4. Our results indicate that Rab28 is a novel GTPase involved in the intracellular retention of GLUT4 in insulin target cells.
Newly translated proteins are substrates for ubiquitin, ISG15, and FAT10
Authors: Spinnenhirn Valentina , Bitzer Annegret , Aichem Annette , Groettrup Marcus
Published: December 2016

The ubiquitin‐like modifier, FAT10, is involved in proteasomal degradation and antigen processing. As ubiquitin and the ubiquitin‐like modifier, ISG15, cotranslationally modify proteins, we investigated whether FAT10 could also be conjugated to newly synthesized proteins. Indeed, we found that nascent proteins are modified with FAT10, but not with the same preference for newly synthesized proteins as observed for ISG15. Our data show that puromycin‐labeled polypeptides are strongly modified by ISG15 and less intensely by ubiquitin and FAT10. Nevertheless, conjugates of all three modifiers copurify with ribosomes. Taken together, we show that unlike ISG15, ubiquitin and FAT10 are conjugated to a similar degree to newly translated and pre‐existing proteins.
3T3‐L1 cells and perivascular adipocytes are not equivalent in amine transporter expression
Authors: Ismail Alex , Ayala‐Lopez Nadia , Ahmad Maleeha , Watts Stephanie W.
Published: December 2016

Rat perivascular adipose tissue (PVAT) stores, takes up, and releases norepinephrine (NE; Ayala‐Lopez et al. (2014) Pharmacol Res Perspect 2, e00041). We hypothesized that 3T3‐L1 adipocytes would exhibit similar behaviors and, thus, could serve as a model for PVAT adipocytes. However, basal levels of NE were not detected in 3T3‐L1 adipocytes. While incubation of 3T3‐L1 adipocytes with exogenous NE increased their cellular NE content, the mRNA expression of several NE transporters [e.g., norepinephrine transporter (NET)] were not detected in these cells. Similarly, we observed expression of the vesicular monoamine transporter 1 (VMAT1) in 3T3‐L1 adipocytes by qRT‐PCR and immunostaining, but stimulation of the cells with tyramine (100 μm) did not cause a significant release of NE. These studies support that 3T3‐L1 adipocytes are not an adequate model of perivascular adipocytes for studying NE handling.
The tumor suppressor inhibitor of growth 4 binds double‐stranded DNA through its disordered central region
Authors: Ormaza Georgina , Medagli Barbara , Ibáñez de Opakua Alain , Rodríguez Jhon A. , Merino Nekane , Villate Maider , Onesti Silvia , Blanco Francisco J.
Published: December 2016

The tumor suppressor inhibitor of growth 4 (ING4) regulates chromatin structure by recruiting the histone acetyl transferase complex HBO1 to sites with histone H3 trimethylated at K4. ING4 dimerizes through its N‐terminal domain and recognizes H3K4me3 by the C‐terminal plant homeodomain (PHD). The central region of ING4 is disordered and contains the nuclear localization signal. Here, utilizing electrophoresis and nuclear magnetic resonance, we show that ING4 binds double‐stranded DNA through its central region with micromolar affinity. Our findings suggest that the cooperativity arising from the presence of two DNA‐binding regions in the ING4 dimer, as well as two H3K4me3‐binding PHD fingers, may strengthen nucleosome binding and HBO1 complex recruitment.
Structural and biochemical characterization of the Clostridium perfringens autolysin catalytic domain
Authors: Tamai Eiji , Sekiya Hiroshi , Goda Eri , Makihata Nahomi , Maki Jun , Yoshida Hiromi , Kamitori Shigehiro
Published: December 2016

Bacterial autolysins can partially hydrolyze cell wall peptidoglycans into small sections to regulate cell separation/division and the growth phase. Clostridium perfringens autolysin (Acp) has an N‐terminal cell wall‐binding domain and a C‐terminal catalytic domain with glucosaminidase activity that belongs to the glycoside hydrolase 73 family. Here, we determined the X‐ray structure of the Acp catalytic domain (AcpCD) at 1.76 Å resolution. AcpCD has a unique crescent‐shaped structure, forming a deep groove for substrate‐binding at the center of the protein. The modeling study of the enzyme/substrate complex demonstrated that the length of the substrate‐binding groove is closely related to the glucosaminidase activity. Mutagenesis analysis showed that AcpCD likely adopts a neighboring‐group mechanism for the catalytic reaction.
SAMI3‐RCM simulation of the 17 March 2015 geomagnetic storm
Authors: Huba J. D. , Sazykin S. , Coster A.
Published: December 2016

We present a self‐consistent modeling study of the ionosphere‐plasmasphere system response to the 17 March 2015 geomagnetic storm using the coupled SAMI3‐RCM code. The novel feature of this work is that we capture the important storm time dynamics of the ionosphere on a global scale and its manifestation in the plasmasphere. We find that the penetration electric fields associated with the magnetic storm lead to a storm time enhanced density in the low‐ to middle‐latitude ionosphere. We compare the modeled total electron content (TEC) with GPS‐measured TEC in the American sector. Additionally, we observe the development of polar cap “tongues of ionization” and the formation of subauroral plasma streams in the postsunset, premidnight sector, and its impact on the plasmasphere. However, we did not see the development of plasmaspheric plumes during this event which we attribute to the long main phase of the storm (∼18 h).
Effects of magnetic drift shell splitting on electron diffusion in the radiation belts
Authors: Zheng Liheng , Chan A. A. , O'Brien T. P. , Tu W. , Cunningham G. S. , Albert J. M. , Elkington S. R.
Published: December 2016

Drift shell splitting in the presence of pitch angle scattering breaks all three adiabatic invariants of radiation belt electron motion and produces new diffusion terms that fully populate the diffusion tensor in the Fokker‐Planck equation. The Radbelt Electron Model (REM) solves such a Fokker‐Planck equation and is used to investigate the phase space density sources. Our simulation results and theoretical arguments suggest that drift shell splitting changes the phase space location of the source to smaller L shells, which typically reduces outer zone phase space density enhancements, and this reduction has a limit corresponding to two‐dimensional local diffusion on a curved surface in the phase space.