bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibitors of benzamidine type influence the virulence properties of Porphyromonas gingivalis strains.
Authors: Eick Sigrun , Pfister Wolfgang , Stürzebecher Uta , Jarema Sigrid , Stürzebecher Jörg
Published: December 2019

Synthetic inhibitors of benzamidine type have been found to have inhibiting effects on arginine specific cysteine proteinases of P. gingivalis. The purpose of our study was to assess the effects of these inhibitors on the virulence properties of two P. gingivalis strains, the reference strain ATCC 33277 and JH16-1, a clinical isolate obtained from a patient with severe periodontitis. The inhibitors tested were pentamidine, benzamidine, three bis-benzamidine derivatives with a pentamidine-related structure, one bis-benzamidine derivative with another structure, and one arginine derivative as a negative control, each in the concentrations of 2 microM and 20 microM. As virulence criteria the following parameters were determined: arginine-specific amidolytic activity, growth inhibition, hemagglutination of sheep erythrocytes, adherence to KB cells and immuno-phagocytosis including intracellular killing. Pentamidine and the bis-benzamidine derivatives with pentamidine-related structure showed the most remarkable effects on reduction of amidolytic activity by 35%, growth inhibition and reduced hemagglutination. Except for the arginine derivative all other inhibitors tested enhanced the phagocytosis capacities of granulocytes. No clear influence of the inhibitors on adherence of P. gingivalis to KB cells was seen. Although in vitro effects of the synthetic inhibitors of cysteine proteinases on virulence of P. gingivalis were observed further in vitro tests concerning immunomodulatory effects should be done before these substances are used for therapy in clinically controlled studies.
Azurocidin -- inactive serine proteinase homolog acting as a multifunctional inflammatory mediator.
Authors: Watorek Wiesław
Published: December 2019

Azurocidin, also known as cationic antimicrobial protein 37 kDa (CAP37) or heparin-binding protein (HBP) is an inactive homolog of serine proteinases residing in granulocytes. The ability to cleave peptide bond was lost due to replacement of two of the three residues from the conserved catalytic triad characteristic for serine proteinases. Azurocidin has a broad spectrum of antimicrobial activity, mainly against Gram-negative bacteria. It is also recognized as a multifunctional inflammatory mediator for its contracting effects on endothelial cells causing an increase of vascular permeability, capacity to bind endotoxin and ability to attract monocytes to inflammation sites.
Non-conventional affinity chromatography of serine proteinases and their inhibitors.
Authors: Polanowski Antoni , Wilimowska-Pelc Anna , Kowalska Jolanta , Grybel Joanna , Zelazko Monika , Wilusz Tadeusz
Published: December 2019

From among a wide variety of protein purification techniques affinity chromatography has proved to be particularly effective for separation of proteolytic enzymes and their inhibitors. In this article, following a general description of affinity adsorbents used for purification of proteinases, we overview a simple separation procedure for some serine proteinases and their inhibitors by way of affinity chromatography in the presence of high NaCl concentration. It has been shown that some highly specific trypsin inhibitors exhibit also antichymotrypsin activity when high concentration of Na(+) but not K(+) or Li(+) ions are present in the reaction mixture. Taking advantage of this phenomenon the virgin forms of trypsin inhibitors from squash seeds, Kazal-type inhibitor from porcine pancreas and alpha(1)-proteinase inhibitor from human and sheep plasma, as an example, were separated using immobilized chymotrypsin or its inactive derivative methylchymotrypsin in the presence of 5 M NaCl.
Desorption/ionization on silicon for small molecules:a promising alternative to MALDI TOF.
Authors: Kraj Agnieszka , Dylag Tomasz , Gorecka-Drzazga Anna , Bargiel Sylwester , Dziubanand Jan , Silberring Jerzy
Published: December 2019

A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, utilizes matrices that need to be mixed with the sample prior to their analysis. Each matrix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addition of a matrix, thus, spectra in the low-molecular mass region can be clearly readable. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous silicon can extend the operating range of a mass spectrometer for studies on metabolomics (small organic molecules and their metabolites, such as chemical neurotransmitters, prostaglandins, steroids, etc.).
Metabolism of cyclic GMP in peritoneal macrophages of rat and guinea pig.
Authors: Kobiałka Marcin , Witwicka Hanna , Siednienko Jakub , Gorczyca Wojciech A
Published: December 2019

The aim of our studies was to establish which enzymes constitute the "cGMP pathway" in rat and guinea pig peritoneal macrophages (PM). We found that in guinea pig PM synthesis of the nucleotide was significantly enhanced in response to activators of soluble guanylyl cyclase (sGC) and it was only slightly stimulated by specific activators of particulate guanylyl cyclases (pGC). In contrast, rat PM responded strongly to atrial natriuretic peptide (ANP), the activator of pGC type A. The rat cells synthesized about three-fold more cGMP than an equal number of the guinea pig cells. The activity of phosphodiesterases (PDE) hydrolyzing cGMP was apparently regulated by cGMP itself in PM of both species and again it was higher in the rat cells than in those isolated from guinea pig. However, guinea pig PM revealed an activity of Ca(2+)/calmodulin-dependent PDE1, which was absent in the rat cells. Using Western blotting analysis we were unable to detect the presence of cGMP-dependent protein kinase 1 (PKG1) in PM isolated from either species. In summary, our findings indicate that particulate GC-A is the main active form of GC in the rat PM, while in guinea pig macrophages the sGC activity dominates. Since the profiles of the PDE activities in rat and guinea pig PM are also different, we conclude that the mechanisms regulating cGMP metabolism in PM are species-specific. Moreover, our results suggest that targets for cGMP other than PKG1 should be present in PM of both species.
Using capillary electrophoresis to study methylation effect on RNA-peptide interaction.
Authors: Mucha Piotr , Szyk Agnieszka , Rekowski Piotr , Agris Paul F
Published: December 2019

Methylation of RNA and proteins is one of a broad spectrum of post-transcriptional/translational mechanisms of gene expression regulation. Its functional signification is only beginning to be understood. A sensitive capillary electrophoresis mobility shift assay (CEMSA) for qualitative study of the methylation effect on biomolecules interaction is presented. Two RNA-peptide systems were chosen for the study. The first one consists of a 17-nucleotide analogue (+27-+43) of the yeast tRNA(Phe) anticodon stem and loop domain (ASL(Phe)) containing three of the five naturally occurring modifications (2'-O-methylcytidine (Cm(32)), 2'-O-methylguanine (Gm(34)) and 5-methylcytidine (m(5)C(40))) (ASL(Phe)-Cm(32),Gm(34),m(5)C(40)) and a 15-amino-acid peptide (named t(F)2: Ser(1)-Ile-Ser-Pro-Trp(5)-Gly-Phe-Ser-Gly-Leu(10)-Leu- Arg-Trp-Ser-Tyr(15)) selected from a random phage display library (RPL). A peptide-concentration-dependent formation of an RNA-peptide complex was clearly observable by CEMSA. In the presence of the peptide the capillary electrophoresis (CE) peak for triply methylated ASL(Phe) shifted from 18.16 to 20.90 min. Formation of the complex was not observed when an unmethylated version of ASL(Phe) was used. The second system studied consisted of the (+18)-(+44) fragment of the trans-activation response element of human immunodeficiency virus type 1 (TAR RNA HIV-1) and a 9-amino-acid peptide of the trans-activator of transcription protein (Tat HIV-1) Tat(49-57)-NH(2) (named Tat1: Arg(49)-Lys-Lys-Arg(52)-Arg-Gln-Arg-Arg- Arg(57)-NH(2)). In the presence of Tat(49-57)-NH(2) a significant shift of migration time of TAR from 18.66 min to 20.12 min was observed. Methylation of a residue Arg(52)-->Arg(Me)(2), crucial for TAR binding, strongly disrupted formation of the complex. Only at a high micromolar peptide concentration a poorly shaped, broad peak of the complex was observed. CE was found to be an efficient and sensitive method for the analysis of methylation effects on interaction of biomolecules.
YOR129c, a new element interacting with Cnm67p, a component of the spindle pole body of Saccharomyces cerevisiae.
Authors: Wysocka Monika , Białkowska Agnieszka , Miciałkiewicz Arkadiusz , Kurlandzka Anna
Published: December 2019

The Saccharomyces cerevisiae spindle pole body (SPB) consists of numerous proteins forming the outer, inner and central plaques. The protein Cnm67 is an important component of the outer plaque. The C-terminus of this protein contains a determinant important for its SPB localization. We identified a protein encoded by YOR129c which interacts with this C-terminus in the two-hybrid system. YOR129c and CNM67 exhibit weak genetic interaction. The double deletion strain yor129cdelta cnm67delta exhibits moderately increased resistance to 0.1M LiCl and hygromycin B compared with the cnm67delta single mutant. We propose that the YOR129c protein is an accessory factor associated with the cytoplasmic face of SPB and plays a role in cation homeostasis and/or multidrug resistance.
Fluorescence of the tri-cyclic adenine and isoguanine derivatives and their ribosides: possible analytical applications
Authors: Stachelska-Wierzchowska Alicja , Wierzchowski Jacek
Published: December 2019

Fluorescent tri-cyclic purine analogs, derivatives of isoguanine and adenine, were examined as potential substrates of purine-nucleoside phosphorylase. It was found previously that etheno- derivatives of both compounds are ribosylated in phosphate-free media, but ribosylation places in some instances differ from purine N9. New ribosides are examined as potential substrates of human blood PNP and indicators of this enzyme. Of these, N6-riboside of 1,N6-etheno-adenine was found the most promising.
FINANCIAL LITERACY AND ITS IMPACT ON THE INVESTMENT DECISIONS OF WORKING WOMEN
Authors: Koti K
Published: December 2019

Purpose of the study: The study was conducted with the main objective of knowing the financial literacy of women in Dharwad District

Methodology: For the research conducted totally of 100 women were interviewed to know their financial ability and proficiency. The statistical tools used where factor analysis, T-Test and One-way Anova.

Main Findings: We could find 30 percent of them were comfortable and found that the gray area was mutual fund investment and return of it. The significant investment avenues which were important and not concentrated were bank deposits and gold markets. They are careful during investments; they know very well about the risk involved in markets and the volatility. They keep financial goals, targets and achieve them successfully. The investment is spread across various portfolios to lower the risk by them

Applications of this study: The study is very important to know the literacy of women in Dharwad District. Based on the study we can create awareness among the women to make them more ease in financial abilities and usage in financial products

Novelty/Originality of this study: The study is unique and researchers or the common person can analysis as to how women think about financial avenues and what is their level of understanding about financial opportunities. Financial institutions can even drive interest in women investing in markets and advisors can make them invest in mutual funds or banking products.
La e-langue des jeunes en France et en Ukraine
Authors: Bilas Andriy
Published: December 2019

Le cadre de cet article est l’étude comparative des nouvelles formes de la langue des jeunes induites par le développement de la communication par Internet et son impact sur la communication et la langue communes. Notre étude s’appuie également sur toute une littérature qui cherche à penser les formes de la langue des jeunes à partir de l’analyse des marqueurs de la e-langue des jeunes en France et en Ukraine afin de dégager la cohérence sociolinguistique des universaux de ce sociolecte. Une approche pluridisciplinaire permet de construire une typologie des formes de la e-langue des jeunes.
USING COMPUTER SIMULATION TO AID THE RESERCH OF DRILLING PROCESSES
Authors: Popova Marina S. , Kharitonov Anton Y. , Parfenyuk Sergey N.
Published: December 2019

Drilling wells is one of the primary methods used for mineral exploration. Scientific studies have aimed at improving the technical and economic aspects of drilling because of the current competitive economic conditions. Note that the primary topic of these studies has been developing new effective rock-cutting tools. To design a new rock-cutting tool, a thorough, reliable, and accurate study of the processes that occur during drilling is necessary. During drilling, mechanical, hydraulic, thermal, and chemical phenomena, which are interdependent and affect the performance of a drilling tool, simultaneously occur; therefore, a systematic, integrated approach is required for studying drilling processes. Field-based and laboratory experiments are quite tedious to perform and require high material costs, and it is often not possible to separately evaluate small elements of the drilling model. Therefore, computer simulation is an important research tool that enables accurate and reliable visualization of even small parts of the model. The aim. The aim of this study is to examine the potential for computer simulation of the processes that occur during drilling. Objective. In this study, we evaluated the simulation features of various software products, such as KOMPAS-3D, ANSYS, Delphi, and LabVIEW, for their utility in studying the processes that occur during drilling. The possibility of computer simulation for studying drilling processes, including its advantages and disadvantages, are demonstrated. The results are obtained from a model that simulates a rock cutting tool. The main uses of the rock cutting tool are outlined, and the drilling simulation development is planned. Choice of research method. The study of the capabilities of existing modern software products, for use in drilling process research, is carried out by an analytical review method.
MOGUĆNOSTI PODSTICANJA KREATIVNOSTI UČENIKA
Authors: Tomčić Lana M.
Published: December 2019

Polazeći od činjenice da je kreativnost važan deo celovite ličnosti, cilj ovog rada jeste pregled mogućih oblika njenog podsticanja u radu sa učenicima. Cilj se ostvaruje primenom metode teorijske analize, uz primenu tehnike analize sadržaja. U radu se polazi od različitih definicija kreativnosti, te bližeg određenja karakteristika kreativnih pojedinaca. Drugi deo rada odnosi se na razmatranje uloge nastavnika u procesu razvoja kreativnosti kroz sledeća pitanja: da li je kreativan nastavnik preduslov za razvoj kreativnosti kod učenika i da li u nastavi kreativnost podstičemo ili je gušimo? Rezultati analize nalaza relevantnih istraživanja i proučavane literature upućuju na određeni broj razrađenih strategija u okviru nastave, vannastavnih i vanškolskih aktivnosti, koji su predstavljeni u radu. U zaključnim razmatranjima predstavljene su pedagoške implikacije proučavanja date problematike.
ALISA OVDE I SADA: SAVREMENA KNJIŽEVNOST U UNIVERZITETSKOJ NASTAVI
Authors: Gordić Petković Vladislava S.
Published: December 2019

Ulazak savremene književnosti u univerzitetsku nastavu nije uvek metodološki jednostavan, iako bi se moglo učiniti da se korišćenjem aktuelnijih narativa koji su, direktno ili ne, odjek recentnih istorijskih i kulturnih okolnosti, postiže adekvatnija korelacija kako sa ostalim nastavnim sadržajima tako i sa ličnim i generacijskim prioritetima studenata. Nedostaje kritička recepcija i književnoteorijska evaluacija koja bi predavaču pomogla da vrednuje književno delo ali, s druge strane, podsticajno je usmeriti studente da sami potraže interpretacijski ključ u ličnom iskustvu i generacijskim opredeljenjima. Analiza romana Uhvati zeca Lane Bastašić ukazuje na mogućnosti uvođenja savremene književnosti u kurikulum.
Editorial - Revista da Fundarte 39ª Edição
Authors: Rhoden Sandra Mara
Published: December 2019

A 39ª edição da Revista da FUNDARTE apresenta dez artigos e dois relatos de experiências de estudos realizados nas áreas de educação, música, teatro, dança e artes visuais. Sob a temática principal em “Educação, Arte e Performance”, pesquisadores se debruçam ao focalizar suas inquietudes centralizadas no fazer artístico e na prática docente,  proposições pedagógico-artísticas, pesquisas coletivas e individuais que contribuem para uma leitura informativa e reflexiva que chama a atenção para uma base teórico-prática direcionada a estudantes, educadores e para aquele que lê para si, ou para o outro.
Cartilha pedagógica
Authors: Brum Lucas Pacheco , Magalhaes Marcos Vinícius
Published: December 2019

O presente trabalho é o resultado de uma reflexão sobre o processo de criação de uma cartilha pedagógica, composta por 07 (sete) fichas com imagens de referência dos/as alunos/as de uma escola da rede pública do Distrito Federal, juntamente com imagens reconhecidas e institucionalizadas do campo da Arte - História da Arte. As fichas contam com 02 (duas) propostas pedagógicas, com questões potentes a partir das imagens, e com dicas de referências de outras visualidades do campo da Arte ou não. Desse modo, os apontamentos teóricos deste trabalho são provenientes dos estudos da Cultura Visual que revelam um discurso pedagógico e sistemático de modo que colocam as visualidades com produtoras se sentidos, discursos, significados e conhecimentos, auxiliando os/as professores/as e arte/educadores em suas práticas pedagógicas cotidianas no trabalho com as imagens. Assim, foi possível estabelecer interfaces e problematizações entre as imagens de referência reconhecidas do universo dos/as alunos/as com as imagens do campo da Arte, tendo como foco os aspectos pedagógicos entre as imagens.
Evaluation of Belgian clays for manufacturing compressed earth blocks
Authors: MANGO-ITULAMYA Lavie A. , COLLIN Frédéric , PILATE Pascal , COURTEJOIE Fabienne , FAGEL Nathalie
Published: December 2019

This study aims to characterize Belgian clays in order to evaluate their use for manufacture of compressed earth blocks (CEB). Nineteen Belgian clay deposits were sampled in 56 sites and 135 samples were collected and analyzed. The analyses focus on the determination of particle size, plasticity, nature and mineralogy as the main characteristics for assessing the suitability of the raw clays to make CEB. These analyses allow for classifying the sampled clay deposits in three categories: clays that can be used unchanged to make CEB (2 clay deposits), clays that are suitable for the manufacture of CEB but require addition of sand and gravel particles (13 clay deposits) and clays that are suitable for the manufacture of CEB if they are mixed with other raw clays (4 clay deposits). In order to verify the use of these clays, five of them served as a model for making CEB. The strength of these bricks was evaluated by testing for compressive strength and abrasion resistance. The results of these tests confirm the suitability or not of the sampled clays for the manufacture of CEB.
Platform capitalism
Authors: Silva Neto Victo José da
Published: December 2019

Nick Srnicek, professor de economia digital do departamento de digital humanities do King’s College, publicou em 2017 o livro Platform capitalism. As linhas de pesquisa do autor são economia marxista, economia de plataformas, políticas antitrabalho e economia política da inteligência artificial.
The art of economic catch-up
Authors: Burcharth Ana
Published: December 2019

Esta é a resenha do livro "The art of economic catch-up: barriers, detours and leapfrogging", publicado em 2019 pelo autor Keun Lee, professor de economia da Seoul National University​. A resenha foi escrita pela autora Ana Burcharth, professora na Fundação Dom Cabral em Nova Lima, Brasil, e professora associada na Aarhus University em Aarhus, Dinamarca.
Extração e comercialização do óleo de andiroba (Carapa guianensis Aublet.) na comunidade da Ilha das Onças, no município de Barcarena, Pará, Brasil
Authors: Sousa Ronaldo Lopes de , Miranda Abdi Ueke da Silva , Cordeiro Yvens Ely Martins , Pereira Maria das Graças
Published: December 2019

Os extratores de óleo das sementes de andiroba residentes na comunidade Ilha das Onças adotam o processo tradicional de extração e emprega, predominantemente, mão de obra feminina. É um produto usado como remédio para inúmeras doenças e a cadeia produtiva tem relevância social e cultural, pois o conhecimento é passado dentro da família e permite relações de convivência.
O uso de plantas medicinais e o interesse pelo cultivo comunitário por moradores do bairro São Francisco, município de Pitangui, MG
Authors: Valeriano Filipe Rodrigues , Savani Fabiana Ramos , Silva Maria Rejane Valeriano da
Published: December 2019

O estudo foi realizado no bairro São Francisco, região urbana de Pitangui, Minas Gerais, com o proposito de investigar o conhecimento popular na utilização de plantas medicinais.  As entrevistas semiestruturadas foram realizadas com 50 famílias residentes no bairro durante o período julho a agosto de 2017. Foram citadas 53 espécies pertencentes a 29 famílias. As folhas foram à parte mais utilizada e a principal forma de preparo é a infusão/chá.
Produção urbana no semiárido brasileiro: um estudo sobre Pau dos Ferros, RN, Brasil
Authors: Carvalho Carla Caroline Alves , Alves Larissa da Silva Ferreira , Sousa Junior Almir Mariano de , Lima Junior Francisco do O de
Published: December 2019

Essa pesquisa objetiva analisar o estabalecimento da morfologia urbana e suas implicações socioespaciais nos bairros São Geraldo e Chico Cajá, situados em zonas de expansão urbana de Pau dos Ferros-RN/Brasil. Como método científico, compreende-se a produção do espaço urbano fomentada dialeticamente pelos diversos interesses e atuações dos agentes produtores do espaço, que proporcionam diferenciações socioespaciais. Enquanto procedimentos metodológicos, utilizou-se de revisão bibliográfica, visitas in loco e análise documental. Para abordagem de dados foi aplicada estatística descritiva. Concluiu-se que a lógica de acumulação capital pauta a produção desses espaços, contribuindo para a valorização imobiliária e para a segregação socioespacial na cidade.
Análise do emprego turístico de Mato Grosso do Sul com o uso de medidas locacionais - 2006 a 2013
Authors: Bernardo Luciana Virginia Mario , Farinha Maycon Jorge Ulisses Saraiva
Published: December 2019

o objetivo da pesquisa é analisar a especialização e a concentração do emprego turístico em Mato Grosso do Sul. Adotaram-se como procedimentos metodológicos medidas de localização. Os resultados indicam especialização do emprego turístico de Mato Grosso do Sul, em alguns municípios. O coeficiente de localização indicou não haver um padrão de concentração. O coeficiente de redistribuição mostrou que não houve mudanças no padrão de localização. E a participação relativa do emprego, não é constante.
Relações de gênero no Acampamento Ilha Verde: discutindo o (des)empoderamento das mulheres beneficiárias do Bolsa Família
Authors: Cecchin Hareli Fernanda Garcia , Parente Temis Gomes
Published: December 2019

A pesquisa teve como objetivo analisar os efeitos do Programa Bolsa Família nas relações de gênero e no empoderamento das mulheres do Acampamento Ilha Verde, localizado em Babaçulândia – TO. Utilizou-se a História Oral para compreender se o programa consegue empoderar essas mulheres nas dimensões pessoal, das relações interpessoais e coletivas. Dessa perspectiva, foram identificados quase todos os fatores inibidores do empoderamento.
Entre política e técnica: reversão, crítica e linguagem em projetos de desenvolvimento
Authors: Radomsky Guilherme Francisco Waterloo
Published: December 2019

O artigo examina o tema do desenvolvimento em perspectiva crítica. Além do seu fracasso ao longo de décadas, mostra que o campo assiste a novas formas de instrumentalização técnica que procuram mitigar sem sucesso aspectos da violência do desenvolvimento. Em geral, a linguagem dos atores principais continua a se apresentar a partir de um lugar de fala que articula hierarquia, saber e poder.
Quality indicators in nutrition therapy within the intensive care setting of a Brazilian teaching hospital
Authors: Rosa Teresa Cristina Abranches , Raslan Mariana , Souza Albert Schiaveto de , Gielow Karine de Cássia Freitas
Published: December 2019

The quality of nutritional therapy was evaluated in an adult intensive care unit in a Brazilian teaching hospital, using ten indicators expressing target quality rates were selected from a list compiled by the Clinical Nutrition Task force of the Brazilian chapter of the International Life Sciences Institute (ILSI-Brasil). It was observed that the use of quality indicators of nutritional therapy allowed the identification of compliance and nonconformity processes, which still need additional adjustments.
Social Observatory as a new space for civil society and government relationships in Belém, Pará State, brazilian Amazon
Authors: Barros Jones Nogueira , Sobrinho Mario Vasconcellos , Vasconcellos Ana Maria de Albuquerque , Cançado Airton Cardoso
Published: December 2019

The article analyses the Social Observatory (SO) as a new arena for civil society, market and government dialogues and incidence on public policy. Specifically, the article examines the extent to which SO is an arena for a constructive dialogue to implement and to manage public policy for urban mobility at municipal level. It deals with the case of SO of Belém municipality, Pará State. Belém is the largest municipality in the Brazilian Amazon region in terms of population and socioeconomic structure for development. The paper shows that OS may constitute a significant participative arena which brings civil society and local government to take collective decision based on dialogue and transparency of information.­­­­ However, since it is a new organizational pattern, the OS still have many difficulties and challenges, whether of internal character, whether in its relation to other actors in the municipal public, private power and civil society.
Contribuições da atividade leiteira para o desenvolvimento rural e para a reprodução da agricultura familiar em um município do sul catarinense
Authors: Dorregão Vandreça Vigarani , Salvaro Giovana Ilka Jacinto , Estevam Dimas de Oliveira
Published: December 2019

Como parte das discussões apresentadas em uma dissertação, desenvolvida no período de 2016-2018, o artigo buscou demonstrar as contribuições da atividade leiteira para o desenvolvimento rural e para a reprodução da agricultura familiar em um município do sul catarinense. Os resultados analisados evidenciaram a relevância da atividade leiteira na região, assim como mudanças significativas nas unidades familiares de produção pesquisadas.
O impacto do e-commerce na venda de bilhetes aéreos: a perspectiva de gestores de agências de viagem de Campo Grande, MS
Authors: Zampieri Rodrigo Adolfo Stefanes , Grechi Dores Cristina
Published: December 2019

Neste trabalho buscou-se verificar o impacto da internet nas vendas de bilhetes aéreos das agências de viagem da cidade de Campo Grande – MS. A abordagem foi no sentido de identificar, sob o ponto de vista do gestor da agência, a influência da tecnologia no hábito dos consumidores. Como base de dados utilizou-se o cadastro do Ministério do Turismo, o qual compreende pessoas físicas e jurídicas atuantes no setor de turismo no país. A metodologia consistiu em pesquisa bibliográfica e de campo. Os resultados confirmam a teoria estudada no que se refere ao impacto da internet no comércio de passagens e no perfil dos novos consumidores.
A social network analysis applied to understand the relationships in fish farming tank-nets business in Brazil
Authors: Martinelli Raiane Real , Pigatto Gessuir , Queiroz Timóteo Ramos , Mollo Neto Mário
Published: December 2019

The present article aims to analyze the insertion of tilapia tank-nets producers in social networks and their influence on the information transfer within the network, from a group of fish farmers located in a reservoir in southeastern Brazil. Exploratory methods characterize this research, which has a qualitative approach based on a multi-case study. Roster method conducts the network research taking into account the limited number of actors in the network and the identification of all. This article allows an understanding of the influence of frequency, time and value attributed to the exchange of information among fish farmers. The trust degree in the relationships between fish farmers was measured through a Social Network Analysis (SNA) with the help of the UCINET® software. The results show that the transmitters of more information are the same that present themselves as central in the network and those who have the most valuable information.
Estilos de liderança predominantes em uma indústria madeireira no Meio-Oeste Catarinense: um estudo de caso
Authors: Gambirage Cinara , Jacomossi Fellipe , Silva Jaison Caetano da , Hein Nelson
Published: December 2019

Este artigo objetiva identificar os estilos de lideranças predominantes em uma indústria madeireira no Meio-Oeste Catarinense, usando dados primários (questionários aplicados com os gestores) e secundários (balanços patrimoniais da empresa). Para análise dos dados primários, aplicou-se a técnica ANACOR, por meio do software LHStat®. Os resultados apontaram que o estilo de liderança transformacional predomina nos gestores da empresa em estudo, poucas vezes fazendo-se presente o estilo laissez-faire.
Terena agriculture and life-system. A speech and beyond
Authors: Maciel Josemar de Campos , Surian Alessio , Brahmllari Estela , Tarasconi Bibiane Ferreira , Antonio Leosmar
Published: December 2019

AbstractThe paper comments on the relationship between the life system of an indigenous community and some issues related to development. It is structured around a core narrative provided by an indigenous leader. His speech is presented and annotated in order to make some of the claims more explicit. Comments are provided in order to contextualize and to elaborate on the oral speech’s intuitive dimension and ancestral wisdom. In the first place the paper seeks a pedagogical tension between reporting the speech as it was delivered to a group of students and key highlights and literature references that intend to expose this indigenous worldview concerning agriculture, education and development to a country such as Brazil that shows difficulties to listen to it. ResumoO artigo comenta a relação entre o sistema de vida de uma Comunidade Indígena Terena e alguns temas relacionados ao Desenvolvimento. Estrutura-se ao redor de uma narrativa de base, um discurso de um líder indígena. Apresentamos a sua preleção, com algumas anotações, para explicitar melhor o seu contexto e o seu alcance. Adicionamos comentários que contextualizam e elaboram reflexões a partir da dimensão mais intuitiva da fala, rica de sabedoria ancestral. O texto persegue uma tensão pedagógica entre a transmissão de uma fala apresentada a um grupo de estudantes, e algumas chaves de leitura e apontamentos que tentam expor o modo indígena de pensar e agir envolvendo a agricultura, educação e o desenvolvimento, em um país que anda tendo dificuldades para ouvir seu próprio povo.
As Representações Sociais da Maternidade para Mães de Filhos(as) com Deficiência
Authors: Crisostomo Kelly Nunes , Grossi Fabiana Regina da Silva , Souza Rafaela dos Santos
Published: December 2019

A descoberta na gravidez de um/a filho/a com alguma deficiência pode trazer efeitos principalmente sobre os sentimentos das mães. Diante disso, objetivou-se analisar as representações da maternidade para mães de filhos/as com deficiência de uma escola pública no município de Barreiras, BA. Trata-se de uma pesquisa qualitativa, descritiva e exploratória. O instrumento utilizado foi a entrevista semiestruturada, e a coleta de dados foi realizada com 14 mães, sendo utilizada a teoria das Representações Sociais para estudo dos dados obtidos. Identificou-se que existe uma busca pelo filho/a “ideal”, e a chegada de uma criança com deficiência gera sentimentos negativos, tais como choque, medo e tristeza. Além disso, elas ainda precisam lidar com as dificuldades em uma sociedade preconceituosa. Portanto deve-se pensar em intervenções e suporte psicológico a fim de diminuir os obstáculos encontrados, bem como promover melhor qualidade de vida para essas mulheres desde o diagnóstico da deficiência da criança.
Primeiro Período de Medicina: Choque de Realidade e o Início da Construção da Identidade Médica
Authors: De Matos Mariana Santiago , Ferraço Camila Mamede , Rosa Julia Carolina Antunes , Bastos Julye Azeredo , Brandão Paula Condé
Published: December 2019

Ao entrar na faculdade de Medicina, o estudante frequentemente possui expectativas que destoam daquilo que ele encontra. Esta pesquisa pretendeu identificar diferenças entre essas expectativas e a realidade, visando compreender mais sobre o início da construção da identidade médica. Trata-se de uma pesquisa qualitativa guiada pelo Método de Explicitação do Discurso Subjacente, da qual participaram 77 alunos do primeiro período do curso de Medicina da Universidade Estácio de Sá, tendo 67 deles respondido a um questionário e 10 sido entrevistados individualmente.  A análise dos dados revelou que os participantes possuíam expectativas idealizadas sobre os colegas e sobre o curso, e a entrada na universidade gerou uma desidealização que parece representar um primeiro passo na aquisição da identidade médica. O contraste entre as expectativas sobre o curso e uma realidade mais árdua do que a esperada, porém, pode ser fonte de sofrimento psíquico, apontando para a necessidade de apoio ao aluno.
Refugees and Trauma
Authors: Dong Yichen
Published: December 2019

Refugees are a group of people who has been leaved their own countries and could not be able to return their own countries safely, which is the result of various reasons, including war, race, religion, nationality, membership of a specific social group as well as political opinion (Dalton-Greyling and Campus, 2008). Due to their miserable and torturous experience, they are viewed as a specific group of people with high incidence of trauma. Therefore, this essay will explain the reasons of examining their experience, find out some factors that contribute to their trauma as well as clarify some issues which should be considered when working with them. Meanwhile, possible intervention approaches and clear reflection will be provided.
Emergency Room Nurses’ Views on Bedside Shift Reporting
Authors: L. Foster Crystal , P. Abraham Samuel , R. Gillum Deborah
Published: December 2019

Bedside shift reporting is a form of communication used by nurses to communicate with each other regarding the patient plan of care. Although bedside shift reporting is required by The Joint Commission and is a required hospital policy, there are inconsistencies in the emergency room nurses performing the task. The purpose of this study was to describe emergency room nurses’ views on bedside shift reporting. A qualitative research study was conducted using a semi-structured interview process. Colaizzi’s data collection and analysis strategy were used to determine emerging themes. Peplau’s interpersonal relations and Benner’s novice to expert theories were used to help guide this study. Fifteen emergency room nurses were interviewed, and seven themes emerged from the data collected. Three themes, nurse accountability, nurse introduction, and patient involvement were identified as benefits of bedside shift reporting. Four themes, bedside shift report not done, emergency room situations, emergency room environment, and time factors were identified as challenges of bedside shift reporting. The study helped to determine the need for additional educational opportunities for the emergency room nurses, emergency department, and the organization to increase the consistency of the reporting process.
Conceptual Design optimization of a Blended Wing Body (BWB) Aircraft Using Flying and Handling Qualities Assessment
Authors: Humphreys-Jennings Clayton , Lappas Ilias , Sovar Dragos Mihai
Published: December 2019

The Blended Wing Body (BWB) configuration is considered to have the potential of providing significant advantages when compared to conventional aircraft designs. At the same time, numerous studies have reported that technical challenges exist in many areas of its design, including stability and control. This study aims to create a novel BWB design to test its flying and handling qualities using an engineering flight simulator and as such, to identify potential design solutions which will enhance its controllability and manoeuvrability characteristics. This aircraft is aimed toward the commercial sector with a range of 3,000 nautical miles, carrying a payload of 20,000kg. In the engineering flight simulator a flight test was undertaken; first, to determine the BWB design’s static stability through a standard commercial mission profile, and then to determine its dynamic stability characteristics through standard dynamic modes. Its flying qualities suggested its stability with a static margin of 8.652% of the Mean Aerodynamic Chord (MAC) and consistent response from the pilot input. In addition, the aircraft achieved a maximum lift-to-drag ratio of 28.1; a maximum range of 4,581 nautical miles; zero-lift drag of 0.005; and meeting all the requirements of the dynamic modes.
Diversity-Generated Image Inpainting with Style Extraction
Authors: Cai Weiwei , Wei Zhanguo
Published: December 2019

The latest methods based on deep learning have achieved amazing results regarding the complex work of inpainting large missing areas in an image. This type of method generally attempts to generate one single "optimal" inpainting result, ignoring many other plausible results. However, considering the uncertainty of the inpainting task, one sole result can hardly be regarded as a desired regeneration of the missing area. In view of this weakness, which is related to the design of the previous algorithms, we propose a novel deep generative model equipped with a brand new style extractor which can extract the style noise (a latent vector) from the ground truth image. Once obtained, the extracted style noise and the ground truth image are both input into the generator. We also craft a consistency loss that guides the generated image to approximate the ground truth. Meanwhile, the same extractor captures the style noise from the generated image, which is forced to approach the input noise according to the consistency loss. After iterations, our generator is able to learn the styles corresponding to multiple sets of noise. The proposed model can generate a (sufficiently large) number of inpainting results consistent with the context semantics of the image. Moreover, we check the effectiveness of our model on three databases, i.e., CelebA, Agricultural Disease, and MauFlex. Compared to state-of-the-art inpainting methods, this model is able to offer desirable inpainting results with both a better quality and higher diversity. The code and model will be made available on https://github.com/vivitsai/SEGAN.
Zn(II) Complex for Highly Sensitive and Selective Detection of Acetone at Room Temperature
Authors: Muddassir Mohd. , Alarifi Abdullah , Usman Mohammad
Published: December 2019

A Zn(II) perchlorate complex has been prepared and characterized by single X-ray crystallography, nuclear magnetic resonance spectroscopy, thermogravimetric analysis, infrared spectroscopy, elemental analysis, and UV-vis spectroscopy. The complex crystallizes in the monoclinic space group P21/c (Z = 4) with a pentacoordinated zinc center. Interestingly, the Zn complex was found be to a potential fluorophore that could sense acetone and other ketones with high selectivity and sensitivity.
Induction and Activity of Terminal Oxidases of Electron Transport Chain in Broccoli Heads under Controlled Atmosphere Storage
Authors: Makino Yoshio , Inoue Jun , Wang Hsiao-Wen , Yoshimura Masatoshi , Maejima Kensaku , Funayama-Noguchi Sachiko , Yamada Takeshi , Noguchi Ko
Published: December 2019

Controlled atmosphere (CA) storage, under atmospheres with low O2 and high CO2 concentrations, is effective for extending the shelflife of horticultural products. We investigated the influence of CA storage (O2/CO2: 2.5%/6.0% or 2.5%/0.0%) at 1C for 21 d versus normoxia (normal air) on the physicochemical and biological properties of broccoli (Brassica oleracea var. italica, Plenck, 1794) via amounts and activities of terminal oxidases of electron transport chain. Mass loss, a sensitive index of freshness for broccoli heads under CA, was significantly lower under CA than under normoxia. The effect for depressing mass loss was observed 7 d earlier under CA including 6.0% CO2 than under CA without CO2. Environmental CO2 was also effective for depressing loss of L-ascorbate. The alternative oxidase (AOX) level under CA was lower than under normoxia during storage, while the level of cytochrome c oxidase (COX), and the AOX/COX activity ratio (based on oxygen isotope discrimination), were stable during storage. Our results indicate that CA storage is effective for retaining freshness of broccoli heads during storage by depressing the induction of AOX. However, depression of AOX level was found to be independent of environmental CO2.
A Critical Discourse Analysis of Major Media Reports about the Issue of the China-US Trade
Authors: Jiaying Wang , Cuiqiong Pan
Published: December 2019

Nowadays, CDA is widely used in news discourse because it can help people understand the implicit viewpoints in news discourse and grasp the true situation of news reports. As the main form of news discourse, media reporting is good intertextuality for CDA. Therefore, this paper, based on the theory of CDA, tries to discuss the implication of major media reporting about the issue of the China-US trade, from which the genre of media reporting here is also analyzed.
The Propeller Flap for Traumatic Distal Lower-Limb Reconstruction
Authors: Lese Ioana , Grobbelaar Adriaan O. , Sabau Dan , Georgescu Alexandru V. , Constantinescu Mihai A. , Olariu Radu
Published: December 2019

Background:

Defects in the distal third of the leg are difficult to cover and often require free tissue transfer, even for defects of limited sizes. Propeller flaps have been designed specifically as an alternative to free tissue transfer but at times have been associated with unacceptably high complication rates. We therefore aimed to prospectively assess our own institutional experience with this technique and to define its role in lower-limb reconstruction.

Methods:

All patients who had been managed with reconstruction of the distal part of the leg with a propeller flap between 2014 and 2017 were included in the study. Demographic, clinical, and follow-up data on the patients and surgical procedures were recorded with special focus on the complication profile.

Results:

Twenty-six patients underwent propeller flap reconstruction of the distal part of the leg: 12 flaps were based on the posterior tibial artery, and 14 were based on the peroneal artery. Postoperative complications developed in association with 1 of the 12 flaps based on the posterior tibial artery, compared with 8 of the 14 flaps based on the peroneal artery (p = 0.015). Moreover, the presence of a higher Charlson comorbidity index (≥2) was strongly associated with the development of postoperative complications (p < 0.001).

Conclusions:

Propeller flaps are a reliable option for traumatic reconstruction in carefully selected patients with lower-limb defects. In our experience, the rate of complications was higher for propeller flaps based on the peroneal artery and for patients with a Charlson comorbidity index of ≥2, whereas posterior tibial artery-based propeller flap reconstruction was a reliable surgical option for patients with a small defect in the distal third of the lower limb.

Level of Evidence:

Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Dose-Dependent Association of Gabapentinoids with Pulmonary Complications After Total Hip and Knee Arthroplasties
Authors: Ohnuma Tetsu , Raghunathan Karthik , Moore Sean , Setoguchi Soko , Ellis Alan R. , Fuller Matthew , Whittle John , Pyati Srinivas , Bryan William E. , Pepin Marc J. , Bartz Raquel R. , Haines Krista L. , Krishnamoorthy Vijay
Published: December 2019

Background:

Gabapentinoids are commonly prescribed in perioperative multimodal analgesia protocols. Despite widespread use, the optimal dose to reduce opioid consumption while minimizing risks is unknown. We assessed dose-dependent effects of gabapentinoids on opioid consumption and postoperative pulmonary complications following total hip or knee arthroplasty (THA or TKA). We hypothesized that use of a gabapentinoid on the day of THA or TKA is associated with an increased risk of postoperative pulmonary complications in a dose-response fashion compared with the risk for patients who did not receive the drug.

Methods:

Using the Premier Database, we identified adults who underwent elective primary THA or TKA from 2009 to 2014. The exposure was receipt of a gabapentinoid (gabapentin or pregabalin) on the day of surgery. Gabapentin dose was categorized into 5 groups: none, 1 to 350, 351 to 700, 701 to 1,050, and >1,050 mg per day. Pregabalin dose was categorized into 4 groups: none, 1 to 110, 111 to 250, and >250 mg per day. The primary outcome was a composite of postoperative pulmonary complications, defined as respiratory failure, pneumonia, reintubation, pulmonary edema, noninvasive ventilation, or invasive mechanical ventilation.

Results:

Of 858,306 patients who underwent THA or TKA, 11.0% received gabapentin and 10.2% received pregabalin. The mean age (and standard deviation) of the patients was 65.6 ± 10.7 years, 39.6% were male, 78.2% were Caucasian, and 55.2% were covered by Medicare. In multilevel regression analysis, receipt of gabapentinoid at any dose on the day of surgery was associated with increased odds of postoperative pulmonary complications. Compared with no exposure to the drug being used by the particular group, all dose ranges of gabapentin and pregabalin were associated with greater odds of postoperative pulmonary complications (odds ratio, 95% confidence interval = 1.51, 1.40 to 1.63, for >1,050 mg of gabapentin and 1.81, 1.57 to 2.09, for >250 mg of pregabalin). We found no clinically meaningful associations between exposure to either gabapentin or pregabalin and perioperative opioid consumption or the length of the hospital stay.

Conclusions:

Exposure to gabapentinoids at any dose on the day of THA or TKA was associated with increased odds of postoperative pulmonary complications in a dose-response fashion, with minimal effects on perioperative opioid consumption.

Level of Evidence:

Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.