bluuebunny/crossref_metadata_2025 huggingface dataset:
ЕФЕКТИВНІСТЬ ВИКОРИСТАННЯ ІНСТРУМЕНТІВ ПАБЛІК РИЛЕЙШНЗ НА ФАРМАЦЕВТИЧНОМУ РИНКУ
Authors: Гуменюк Алла , Гарматюк Олена
Published: April 2023

The article examines the effectiveness of the use of public relations tools in the pharmaceutical market, summarizes the reasons that have a destructive effect on the activities of its participants. The concept of public relations management is developed,
ДОСЛІДЖЕННЯ ВИХОВНОГО ЗНАЧЕННЯ МОРАЛЬНИХ ЦІННОСТЕЙ ПІДРОСТАЮЧОГО ПОКОЛІННЯ ЗАСОБАМИ НАРОДОЗНАВСТВА
Authors: Лебідь В.П. , Харченко І.М.
Published: April 2023

У статті представлено напрями психологічної антропології, який розглядає специфіку сприйняття, пізнання, мислення, моральних цінностей та їх виховання в учнів. Пропонується педагогічне обґрунтування необхідності виховання моральних цінностей на народному
ПРОЦЕС ДИВЕРСИФІКАЦІЇ ЯК УПРАВЛІННЯ ПРОФЕСІЙНОЮ ПІДГОТОВКОЮ МАЙБУТНІХ ФАХІВЦІВ
Authors: Небикова Т.А. , Небиков М.В.
Published: April 2023

У статті запропоновано поняття диверсифікації, яке використовують в літературі, пов’язаній з проблемами освіти. Узагальнено різні види диверсифікації. Визначено диверсифікацію освіти майбутнього фахівця як педагогічну проблему.
ПРОЦЕС ПРОЕКТУВАННЯ ОСВІТНЬОГО ПРОСТОРУ ЗАКЛАДІВ ДОШКІЛЬНОЇ ОСВІТИ ЯК УМОВА РОЗВИТКУ ДИТИНИ
Authors: Обміняна Г.Л. , Яценко Тамара
Published: April 2023

У статті пропонується розробка теоретичної моделі освітнього простору різними засобами з урахуванням сучасних умов, методів і засобів проектування, а також меж професійної діяльності педагога, що дозволяє визначити основні принципи і поняття для реалізаці
ПСИХОЛОГІЧНІ ФАКТОРИ ВПЛИВУ НА ЕФЕКТИВНУ ДІЯЛЬНІСТЬ ПЕДАГОГА
Authors: Піковець Н.В. , Хорольська О.Г.
Published: April 2023

У статті визначено психологічні фактори впливу, які визначають ефективну діяльність педагогів. Узагальнено критерії загального розвитку особистості. Підкреслено, що в основі мотивів діяльності педагога лежать певні потреби. Розглянуто ціннісні характе
РОЗВИТОК ПІЗНАВАЛЬНИХ ІНТЕРЕСІВ ДІТЕЙ З ІНТЕЛЕКТУАЛЬНИМИ ПОРУШЕННЯМИ НА УРОКАХ ПРИРОДОЗНАВСТВА
Authors: Штангей С.В. , Поштарук Л.І.
Published: April 2023

У статті на основі аналізу наукової літератури розкрито поняття «пізнавальний інтерес» у психолого-педагогічній літературі та проблема розвитку пізнавального інтересу в школярів з інтелектуальними порушеннями.
КОМУНІКАТИВНА КОМПЕТЕНТНІСТЬ МАЙБУТНЬОГО СОЦІАЛЬНОГО ПРАЦІВНИКА У ЗМІСТІ ФАХОВОЇ ПІДГОТОВКИ
Authors: Соболенко О.І. , Крикунова Елена
Published: April 2023

У статті проаналізовано зміст і структуру комунікативної компетентності. Висвітлено складові компоненти комунікативної компетентності фахівця. Обґрунтовано актуальність і необхідність застосування компетентнісного підходу у системі освіти. Роз
ІНКЛЮЗИВНА ОСВІТА ЯК ШЛЯХ ГУМАНІЗАЦІЇ СУСПІЛЬСТВА
Authors: Фещенко Галина , Малышева Леся
Published: April 2023

У статті проаналізовано сутність інклюзивної освіти, її значення для соціалізації дітей з особливими потребами. Звертається увага на особливості інклюзивних процесів в українській освіті, важливість повноцінної реалізації інклюзивної освіти. Якісне зді
KNOWLEDGE EXTRAPOLATION OF EDUCATIONAL-DIGITAL AND ADMINISTRATIVE «MECHANISMS» OF TEACHING IN HIGH SCHOOLS: MOTIVATION AND ORGANIZATIONAL CONSTRUCTIVISTICS OF EDUCATIONAL AND SCIENTIFIC PRACTICES IN THE PROCESS OF TRAINING FUTURE SCHOOL TEACHERS
Authors: Bodyk Ostap , Salahub Lyudmila
Published: April 2023

The paper examines core concepts in the context of the cognitive extrapolation of digital and management «mechanisms» to the teaching process in higher education through the implementation of motivational and organizational constructivist educational and
АДМІНІСТРАТИВНО-ПРАВОВОВЕ РЕГУЛЮВАННЯ ПУБЛІЧНОЇ
Authors: Grutsenko Yu.
Published: April 2023

The article states the need for consistent improvement of the system, organizational structure and competence of public service in the law enforcement sphere of Ukraine, correction of the legislative and organizational and management principles of their f
ELECTRIC CURRENT AND ITS PROPERTIES
Authors: Anisimov Nikolay
Published: April 2023

Electrical engineering is the science of technical use of electrical phenomena. One of the most important branches of electrical engineering - electrical engineering studies the use of electricity to obtain large amounts of energy in places where there ar
MODELING OF "SOFT SKILLS" IN THE SYSTEM OF MANAGERIAL COMPETENCE
Authors: Cherusheva Halyna
Published: April 2023

The article discusses soft skills and their role in the activities of future managers, since possession of academic skills alone is not sufficient for successful management and career growth. The concept of flexible skills is defined, their importance and
Characterisation of new animal cell cultures’ sensitivity to Coxsackievirus B5 and Herpes simplex virus‑1
Authors: Zakharova Yu. A. , Ostapchuk A. V. , Wasielewski W. W. , Fedotova O. S. , Shmeleva N. A.
Published: April 2023

The increase in the number of cell cultures for virology and biotechnology enhances the chances of a successful response to threats related to outbreaks of well-known and new human infectious diseases. It is a vital task to search for cell cultures sensitive to a wide spectrum of viruses.

The aim of the study was to investigate the sensitivity of new diploid animal cell cultures (fibroblasts of a foetal pig’s kidneys and larynx) to Coxsackievirus B5 (CVB5) and Herpes simplex virus-1 (HSV-1).

Materials and methods. The cultures of porcine foetal kidney fibroblasts (PFKF) and porcine foetal larynx fibroblasts (PFLF) were derived from a foetus of a healthy pig by mild trypsinisation. The study determined the sensitivity of these new PFKF and PFLF cultures to the above-mentioned viruses by the cytopathic effect (CPE) expressed as a percentage. The infectious activity of CVB5 was studied using real-time polymerase chain reaction (PCR) with the determination of amplification cycle threshold values (Ct); that of HSV-1 was studied using quantitative titration of the virus-containing liquid (VCL). Infectious activity values were expressed as tissue culture 50% infective doses (TCID50).

Results. The authors developed diploid PFKF and PFLF cell cultures. PFKF cells demonstrated high sensitivity to CVB5, with a CPE of 87.5±3.3% after passage 3 and a satisfactory concentration of enterovirus RNA in the VCL of 22–24 Ct . The sensitivity of PFKF cells to HSV-1 corresponded to a CPE of 92.1±5.5%. In these cells, the infectious activity of HSV-1 corresponded to 104.25 TCID50/0.2 mL. The experiments with PFLF cells showed low CPE and infectious activity values for both viruses.

Conclusions. The study demonstrated high CPE values with the CVB5 (CB5-8100) and HSV-1 (HSV-1/L-2) strains as examples and confirmed the sensitivity of the new diploid PFKF cell culture to these test viruses. Thus, the PFKF cell culture offers potential applications in virology and biotechnology and may be a candidate for testing other strains of CVB5 and HSV-1.
Chikungunya vaccines: advances in the development and prospects for marketing approval
Authors: Otrasheuskaja E. V. , Trukhin V. P. , Merkulov V. A. , Ignatyev G. M.
Published: April 2023

Chikungunya fever is an acute infectious disease caused by the mosquito-borne Chikungunya virus (CHIKV). In the last decades, cases of the disease have been reported in more than 100 countries; therefore, CHIKV presents a global public health problem. CHIKV genotypes have limited antigenic diversity, and documented reinfection is very rare. Hence, a vaccine could prevent infection and potential disability, as well as reduce the epidemic spread of CHIKV in the population.

The aim of the study was to review approaches to the development of preventive vaccines against CHIKV, evaluate promising vaccine candidates in preclinical or clinical development stages, and analyse perspectives and challenges of bringing these vaccines to the pharmaceutical market.

According to the literature reviewed, both traditional and modern platforms are used in the development of CHIKV vaccines, which has been ongoing for several decades. Each platform has its advantages and limitations. The most popular platforms are live attenuated vaccines and vaccines with viral vector constructs. To date, about 25 vaccine candidates have successfully passed through preclinical studies, and more than 7 vaccine candidates have progressed to various phases of clinical studies. The preventive medicinal products that have reached the clinical development stage include 4 live attenuated vaccines, 1 inactivated vaccine, 1 vaccine containing virus-like particles, and 1 mRNA vaccine. All 7 candidates have demonstrated cross-protection against multiple genotypes of CHIKV at the level of either preclinical in vivo studies and/or clinical in vitro studies. The research continues, and this shows that not only the scientific community but also health systems are interested in bringing effective CHIKV vaccines to the pharmaceutical market.
Analysis of approaches to sterility testing of COVID-19 prevention vaccines
Authors: Sukhanova S. M. , Tikhonova A. S. , Berdnikova Z. E.
Published: April 2023

Preventive vaccination against SARS-CoV-2 infection is currently receiving close attention in the Russian Federation. Improving public confidence in immunisation with new vaccines largely depends on a guarantee of the absence of side effects caused by contamination. A high risk of contamination is inherent to biological products, including coronavirus prevention vaccines, due to their properties and the nature of raw materials used. This risk adds to the need for using effective contaminant detection approaches.

The aim of the study was to evaluate the possibility to improve sterility testing of preventive vaccines against SARS-CoV-2 infection.

This article presents an analysis of the procedures proposed by pharmaceutical developers for sterility testing of ten Russian vaccines approved in the country for COVID-19 prevention. The authors considered specific characteristics of these vaccines, including their physical and chemical properties, the presence of antimicrobial components, and other critical factors affecting the correctness of the experimental setup. The results suggest that it is possible to improve sterility testing. According to the authors, the main directions for its improvement are the proposal to develop an alternative procedure based on compendial method 2 (OFS.1.2.4.0003.15, Ph. Rus. XIV), as well as the use of a universal culture medium. If used for refining the established procedures and developing new ones, the authors’ recommendations will improve the reliability and applicability of sterility testing during both manufacturing and pre-approval regulatory assessment of updated coronavirus vaccines for subsequent release to the market. The proposed approaches can be applied to testing other medicinal products for sterility.
Current regulatory requirements for non-clinical evaluation of prophylactic vaccines
Authors: Gorenkov D. V. , Komarovskaya E. I. , Soldatov A. A. , Avdeeva Zh. I. , Bondarev V. P.
Published: April 2023

Vaccines are subject to specific regulatory requirements for the evaluation of their quality, safety, and efficacy. In 2005, the World Health Organisation (WHO), as the main international organisation coordinating measures to combat infectious disease outbreaks, began developing documents on the evaluation of vaccine quality, safety, and efficacy. The world’s leading regulatory authorities (FDA, EMA, etc.) have also issued recommendations for conducting non-clinical studies of vaccines.

The aim of this study was a critical review of the regulatory requirements established by foreign national and international regulatory authorities for non-clinical evaluation of the safety and efficacy of vaccines.

According to the study results, since the 2000s, the WHO and the world’s leading regulatory authorities have produced more than 40 regulatory documents describing certain aspects of non-clinical studies of the safety and efficacy of vaccines. These documents can be divided into two groups: the first group addresses non-clinical studies of vaccines in general, and the second one dwells upon the evaluation of the quality, safety, and efficacy of specific types of vaccines. For the Russian guidelines on non-clinical evaluation of the quality, safety, and efficacy of immunobiologicals, the latest revision dates back to 2013 and does not provide any information on new medicinal products. Currently, work is underway to prepare the regulatory framework for medicines, including vaccines, in the Member States of the Eurasian Economic Union (EAEU). This review of regulatory documents on non-clinical safety and efficacy studies of vaccines may be useful in drafting harmonised guidelines for the relevant groups of vaccines in the EAEU. It may also be of use to developers, manufacturers, and researchers involved in the creation and non-clinical study of vaccines.
Evaluation of the diagnostic efficacy of a reagent kit for in vitro diagnosis of West Nile fever using reverse transcription polymerase chain reaction with fluorescent probe-based detection
Authors: Prokhvatilova E. V. , Tkachenko G. A. , Baturin A. A. , Belitskaya L. I. , Toporkov A. V.
Published: April 2023

West Nile fever is a vector-borne zoonotic arbovirus infection with natural foci. Its clinical course is similar to that of acute febrile syndrome, and severe cases may result in neuroinvasive disease. Several genetic lineages (1, 2, and 4) of the West Nile virus (WNV) with different pathogenicity for humans are circulating in the Russian Federation. Therefore, it is an urgent task to develop a diagnostic reagent kit for differentiating between WNV genetic lineages and to implement the kit in clinical laboratory practice.

The aim of the study was to conduct technical and clinical tests and evaluate the quality, efficacy, and safety of the Ampligen-WNV-genotype-1/2/4 diagnostic reagent kit for detecting WNV RNA and differentiating between WNV genetic lineages 1, 2, and 4 by reverse transcription polymerase chain reaction (RT-PCR) with fluorescent probe-based detection.

Materials and methods. The authors determined the diagnostic sensitivity and specificity of the Ampligen-WNV-genotype-1/2/4 reagent kit (Volgograd Research Institute for Plague Control, Russia) by real-time RT-PCR with 216 clinical samples and 204 biological samples. Sanger sequencing was used as a reference method. Statistical analysis of clinical test results was carried out in accordance with the Russian national standard for clinical laboratory tests (GOST R 53022.3-2008).

Results. When tested with the Ampligen-WNV-genotype-1/2/4 reagent kit, real-time RT-PCR demonstrated the analytical sensitivity of 1×104  GEq/mL for the detection of WNV cDNA of genetic lineages 1, 2, and 4. The assessment of its analytical specificity showed no positive results for cDNA samples of heterologous viruses at a concentration of 1×106  GEq/mL. The diagnostic sensitivity with the reagent kit was at least 98.5%, and the diagnostic specificity was at least 99%, with 90% confidence levels for both parameters.

Conclusions. The Ampligen-WNV-genotype-1/2/4 reagent kit can be recommended for use in clinical laboratory diagnostics to detect WNV RNA and differentiate between WNV genetic lineages 1, 2, and 4.
Self-concept on @Nge.review Instagram Account as a Representation of User Character
Authors: Sanubari Farrasepta Deandra , Surahman Sigit , Poetra Yudhistira Ardi
Published: April 2023

This study describes the Instagram account @Nge.review as one of the bookstagram accounts. Bookstagrammers are Instagram users who are obsessed with physical books where the object is the object in every upload on Instagram. Like other bookstagram accounts, each of these accounts has tens of thousands of followers and hundreds of uploads and, each upload has tens of thousands of likes. As one of the bookstagrammers, the owner and user here has differences with other bookstagram account users in the form of uploaded photos, the contents of the reviewed book reviews and the contents of the Instagram features contained in the account. The researcher aims to find the self-concept and character behind the habits that are shown through the contents of the account. This study uses a qualitative approach. On data search using interview techniques, observation and research documentation. Based on the research that has been done, it shows that the user as well as the owner of the @Nge.review Instagram account has a smart and imaginative self. The characters found in the user’s account that is, have an open personality.Keywords: Self concept, representation, character, Instagram ABSTRAKPenelitian ini menjelaskan akun Instagram @Nge.review sebagai salah satu akun bookstagram. Bookstagrammer adalah para pengguna Instagram yang terobsesi dengan buku fisik yang dimana benda tersebut menjadi objek dalam setiap unggahan di Instagram. Layaknya akun bookstagram lainnya, akun Instagram memiliki pengikut puluhan ribu dan ratusan unggahan serta, setiap unggahan ada yang memiliki belasan ribu likes. Sebagai salah satu bookstagrammer, pemiliki sekaligus penggunanya disini memiliki perbedaan dengan pengguna akun bookstagram lain dalam bentuk foto unggahan, isi ulasan buku yang diresensi serta, isi dari fitur-fitur Instagram yang terdapat pada akun. Peneliti bertujuan untuk mencari konsep diri dan karakter di balik kebiasaan yang diperlihatkan melalui isi dari akun tersebut. Penelitian ini menggunakan pendekatan kualitatif. Pada pencarian data menggunakan teknik wawancara, observasi dan dokumentasi penelitian. Berdasarkan penelitian yang telah dilakukan menunjukkan hasil bahwa, pengguna sekaligus sebagai pemilik akun Instagram @Nge.review memiliki diri yang pintar dan imajinatif. Karakter yang ditemukan pada pengguna akun yaitu, memiliki pribadi yang terbuka. Kata Kunci: Konsep diri, representasi, karakter, Instagram
The Wisdom of Sundanese Intercultural Communication in Social Interaction with Javanese Ethnic Communities
Authors: Chalik Alex Abdu , Arrianie Lely , Sarofah Riska
Published: April 2023

This study aims to explain the forms of social interaction between the Sundanese and Javanese ethnicities that take place in the form of adaptation, cooperation, tolerance, competition, and conflict of intercultural communication wisdom. This research was conducted in Kabawetan District, Kepahiang Regency, Bengkulu Province. The novelties in the research include obtaining an overview and explanation of the history of the migration of the Sundanese to Sukasari Village, forms of social interaction between the Sundanese ethnic community and the Javanese ethnicity, and the implementation of Sundanese cultural wisdom in intercultural communication with the Javanese. This study uses a qualitative approach through in-depth interviews with informants to explore information about the wisdom of Sundanese intercultural communication in social interaction with the Javanese ethnic community. The technique of taking informants is done by using the snowball sampling technique according key informants who will recommend other sources.. The results showed that the intercultural communication wisdom of the Sundanese in social interaction with the Javanese ethnic community has been carried out well through babasan and paribasa owned by the Sundanese ethnic community within its cultural framework.Keywords: Social interaction, ethnicity, intercultural communication, local wisdom ABSTRAKPenelitian ini bertujuan untuk menjelaskan bentuk interaksi soial antara etnis Sunda dengan etnis Jawa yang berlangsung dalam bentuk adaptasi, kerjasama, toleransi, kompetisi dan konflik kearifan komunikasi antarbudaya. Penelitian ini dilakukan di Kecamatan Kabawetan, Kabupaten Kepahiang, Provinsi Bengkulu. Kebaruan dalam penelitian antara lain untuk memperoleh gambaran dan penjelasan tentang sejarah migrasi orang Sunda ke Desa Sukasari, bentuk-bentuk interaksi sosial komunitas etnis Sunda dengan etnis Jawa, dan implementasi kearifan budaya Sunda dalam komunikasi antarbudaya dengan etnis Jawa. Penelitian ini menggunakan metode pendekatan kualitatif melalui wawancara yang mendalam dengan informan untuk menggali informasi mengenai kearifan komunikasi antarbudaya Orang Sunda dalam interaksi sosial dengan komunitas etnis Jawa. Teknik pengambilan informan dilakukan dengan Teknik snowball sampling melalui beberapa narasumber kunci untuk merekomendasikan informan lainnya. Hasil penelitian menunjukkan bahwa kearifan komunikasi antarbudaya orang Sunda dalam interaksi sosial dengan komunitas etnis Jawa telah dijalankan dengan baik melalui babasan dan paribasa yang dimiliki oleh komunitas etnis Sunda dalam kerangka kebudayaannya.Kata Kunci: Interaksi sosial, etnis, komunikasi antarbudaya, kearifan local
Effect evaluation of supramolecular complex of ivermectin Aniverm-2.0% on postnatal development of rat offspring
Authors: Zashchepkina V. V. , Musaev M. B.
Published: April 2023

The purpose of the research is to detect long-term effects of repeated oral administration of supramolecular complex of ivermectin Aniverm-2.0% on the postnatal development of rat offspring.Materials and methods. The studies were conducted on 16 white pregnant rats that were divided into two experimental and one control groups. The animals were kept under standard conditions of keeping and feeding. The supramolecular complex of ivermectin Aniverm-2.0% was administered to pregnant female rats (n = 6) of group 1 in the form of a suspension using an intragastric tube daily for 7 days at a dose of 15 mg/kg, and group 2 was given the substance of ivermectin (n = 5) at a dose of 8.25 mg/kg. The control female rats (n = 5) were administered 1 mL of distilled water during the experiment. The experimental pregnant females were left until delivery, and then, the development of their offspring was monitored for 45 days. After rat pups were born, the following were recorded: pregnancy duration, litter size, dynamics of weight gain in the rat pups for 21 days, postnatal death during the first 30 days, the ratio of males and females in the litter, periods of eye opening, incisor eruption, detachment of the auricle, appearance of hair coat, descent of testicles, and opening of the vagina. Then we assessed the maturation rate of sensory-motor reflexes in the offspring obtained from the experimental and control groups, the emotional motor behavior and ability for fine coordination of movements in the offspring, and conducted the open field-2 test on day 45 after birth.Results and discussion. No negative effect was detected for supramolecular complex of ivermectin Aniverm-2.0% on the parameters of physiological development of the offspring of the experimental rats within 45 days after birth. The dynamics of their mass, developmental parameters, and formation of motor reflexes during the feeding period remained within the normal values. Developmental indicators of sensory motor reflexes in the control and experimental rat pups had no statistically significant differences.
Biodiversity of helminths in Karakul sheep in the Khorezm Oasis of Uzbekistan
Authors: Akbarov A. A. , Akramova F. J. , Shakarbayev U. A. , Azimov D. A.
Published: April 2023

The purpose of the research is to study the helminth fauna and the structure of helminthocenoses of Karakul sheep in the Khorezm Oasis of Uzbekistan.Materials and methods. The material for this study was helminths collected by conventional methods from Karakul sheep from farms of different types in the Khorezm Region. A total of 13 sheep were studied. To identify the dominant helminth infections in sheep, 110 sets of individual organs were examined by the method of partial helminthological dissections. Helminths were collected in slaughterhouses of the Bagat, Kushkupyr, Urgench, Yangiaryk and Shavat Districts of the Khorezm Region. Additionally, the helminths collected from sheep in the Khorezm Region for 2015–2021 were used.Results and discussion. We found that the helminth fauna of Karakul sheep from the Khorezm Oasis was represented by 22 species of the classes Cestoda, Trematoda and Nematoda. Cestodes were represented by 6 species, trematodes by 3 species, and nematodes by 13 species. The faunistic composition of helminths in studied animals was noticeably depleted as compared to other Uzbekistan regions due to natural and ecological conditions of the Khorezm Oasis. The total helminth infection rate in sheep was 100%. The intensity of infection varied depending on the season of the year and the age of sheep and ranged from tens to several thousand specimens. Almost all organs and systems of the animals were found to be ecological niches of the above helminths. Most of helminth species were common to both domesticated and wild ungulates.
Optimal schemes for the use of anthelmintics at gastrointestinal strongylatosis of young cattle
Authors: Arkhipov I. A. , Varlamova A. I. , Kachanova E. O.
Published: April 2023

The purpose of the research is to study the rational terms for the use of anthelmintics in case of gastrointestinal strongylatoses of young cattle in Nonchernozem zone of the Russian Federation.Materials and methods. The timing of the onset of re-isolation of nematode eggs with feces of cattle after the use of some anthelmintics was determined on 50 black-motley calves weighing 117–130 kg, spontaneously infected with gastrointestinal strongylates. The optimal schemes for the use of anthelmintics in case of gastrointestinal strongylatosis of young cattle were studied on 109 calves of the first year of grazing in the pasture period of 2010. After numbering, the animals were weighed and divided into experimental and control groups. Each group was divided into two subgroups of 15–17 heads. Animals of all groups from May 3 to October 10, 2018 were grazed together in one herd on a pasture where cattle infected with strongylates had previously grazed. Levamisole from the group of imidothiazoles, Alben from the group of benzimidazoles and Aversect-2 from the group of macrocyclic lactones were tested. The drugs were used in therapeutic doses: levamisole at a dose of 7.5 mg/kg by AS intramuscularly, Alben at a dose of 7.5 mg/kg with concentrated feed, and Aversect-2 at a dose of 0.2 mg/kg subcutaneously once. Levamisole was administered to the animals of the first group on the 6th, 10th and 14th weeks of grazing, and to the animals of the second group - on the 6th and 10th weeks of grazing. Alben was used on young cattle of the third group on the 6th, 11th and 16th weeks, and on the animals of the fourth group - on the 6th and 11th weeks of grazing. Aversect-2 was administered to cattle of the fifth group at the 6th, 13th and 20th weeks of grazing, and to the animals of the sixth group – at the 6th and 13th weeks. Animals of the 7th group did not receive the drug and served as control. Fecal samples were taken from animals of all groups on a monthly basis and investigated by a quantitative method in order to detect eggs of gastrointestinal strongylate. The results obtained were statistically processed using the computer program Microsoft Excel.Results and discussion. Tests of drugs for gastrointestinal strongylatoses of young cattle showed different persistence of anthelmintic action. After deworming the animals, single eggs of strongylate in their faeces were again detected 4 weeks after the administration of Levamisole, 5 weeks after the administration of Alben, and 7 weeks after the administration of Aversect-2. The most rational scheme for deworming young cattle with gastrointestinal strongylatoses is the use of Aversect-2 on the 6th and 19th weeks of grazing.
The efficacy of complex solid dispersions of anthelmintics against experimental trichinellosis
Authors: Varlamova A. I. , Khalikov S. S. , Meteleva E. S. , Evseenko V. I. , Khalikov M. S. , Arkhipov I. A.
Published: April 2023

The purpose of the research is to study the influence of various technological factors on obtaining of complex solid dispersions of anthelmintics with polyvinylpyrrolidone and licorice extract on anthelmintic efficacy in experimental trichinellosis of white mice.Materials and methods. The study of the nematodocidal activity of complex solid dispersions samples based on fenbendazole (FBZ), fenasal (FNS) and praziquantel (PZQ) with polyvinylpyrrolidone (PVP) and licorice extract (LE) obtained by mechanochemical technology at different ratios of components and different exposure times was carried out on 130 white mice experimentally infected with Trichinella spiralis in two experiments. On the 3rd day after infection, the animals were divided into experimental groups of 10 animals each. Samples of various complex solid dispersions of anthelmintics were administered intragastrically to the mice of the experimental groups at a dose of 2 mg/kg according to the active substance. FBZ substance was used as the basic drug at a dose of 2 mg/kg according to the active substance. Animals of the control groups did not receive the drugs. The animals were killed by decapitation on the 4th day after experimental drug samples administration, and the activity of the drugs was counted according to the results of helminthological necropsy of the intestine, the efficacy was calculated by the type of control test.Results and discussion. The efficacy of complex solid dispersions of FBZ and FNS with PVP polymer was higher in comparison with the activity of complexes with LE at the same duration of mechanochemical treatment in a roller mill. The FBZ activity decreased from 67.05 to 37.77% with a decrease in the duration of mechanochemical treatment from 24 h to 5 h and the efficacy of the FBZ : FNS complex with LE turned out to be almost at the level of the basic drug when treated for 1 h. The use of mechanochemical technology for obtaining of a solid dispersion of FBZ : FNS with PVP for targeted delivery makes it possible to increase the anthelmintic efficacy by 2.7 times compared with the activity of the FBZ substance, and with LE by 2.2 times. It was noted that complex solid dispersions of PBZ with PZQ have lower biological activity in comparison with compositions of FBZ with FNS.
Experience of eradicating parasites of laboratory rats in conventional vivarium
Authors: Polukhina D. N. , Panova O. A. , Kurnosova O. P. , Emelyanova N. B. , Sysoeva N. Yu. , Khrustalev A. V. , Kachurina L. I.
Published: April 2023

The purpose of the research is to test different dehelminthization schemes of laboratory rats infected with cestodes Rodentolepis nana and nematodes Syphacia muris and evaluate the significance of combined environment disinfection measures. The practical experience of eradication (helminth eradication) in animals in a conventional vivarium was described.Materials and methods. Experiments were conducted to study the efficacy of anthelmintics and administration schemes against cestode and nematode infections in laboratory rats. In the first experiment, praziquantel was used at a dose of 10 mg/kg to treat rats infected with R. nana. In the second experiment, the comparative efficacy of fenbendazole, albendazole, and pyrantel was evaluated against syphaciosis at the recommended dosages of 20, 10, and 12.5 mg/kg, respectively. Each drug was given orally, individually, twice with an interval of 7 days. The third experiment tested different schemes for treating syphaciosis with fenbendazole. One group of rats was given the drug orally individually using an esophageal tube at a dose of 20 mg/kg once a day for 7 consecutive days. Other groups were given fenbendazole daily with food for 7 days (150 mg fenbendazole per 1 kg of food). In all three experiments, all animals were divided into groups, and their cells underwent a complex of additional disinfection measures, and those kept in cages without disinfection.Results and discussion. Praziquantel showed 100% efficacy at a single dose of 10 mg/kg in R. nana therapy. In animals without additional disinfection procedures, cestode eggs were again recorded starting from day 14 after the drug administration. In the group of animals with disinfection measures, pathogens were not detected during the experiment. Double administration of fenbendazole, albendazole and pyrantel in the recommended dosages against syphaciosis did not result in eradicated nematodes in the animals. The disinfection did not affect the obtained results. Fenbendazole administered daily for 7 days ensured helminth eradication in animals. However, on day 7 after the therapy, Syphacia sp. eggs were again found in the groups that received the drug individually intragastrically through a tube, regardless of whether their cells were disinfected. The animals that received fenbendazole with food and were regularly disinfested remained free from nematodes throughout the experiment until the additional disinfection measures were cancelled. In the absence of disinfection, released helminth eggs were recorded on day 14 after therapy.
Species composition and ecology of blood-sucking mosquitoes (Diptera, Culicidae) in Yakutia
Authors: Reshetnikov A. D. , Barashkova A. I.
Published: April 2023

The purpose of the research is to study the species composition and ecology of mosquitoes in Yakutia.Materials and methods. The research was performed for herd horses and cattle on pastures in Central, Western and Southern Yakutia in 2005–2021. Faunistic collections were made and the number of blood-sucking mosquito imago was recorded for animals using an entomological net with removable bags, while the average abundance rate of mosquitoes was calculated per 10 net movements in a figure-eight pattern around from 10 repetitions when studying the seasonal abundance dynamics and from 5 repetitions when studying the circadian activity rhythm.Results and discussion. In Yakutia, 15 species of blood-sucking mosquitoes of the family Culicidae were found that belong to three genera: Anopheles, Culiseta, and Aedes. Biotopes occupied by mosquito larvae of the genus Aedes include 74–126 specimens/m2. The first mosquitoes were recorded near an animal bait in the first decade of May. The mass mosquito flight period was observed from the third decade of June to the middle of the second decade of July. The total mosquitofl ight period for the season was 121–124 days. The flight of blood-sucking mosquitoes on the forest pasture continues around the clock, from the third decade of June to the middle of the second decade of July.
Trematode fauna of fish inhabiting reservoirs of the European part of Russia
Authors: Romanova N. N. , Golovina N. A. , Vishtorskaya A. A. , Golovin P. P.
Published: April 2023

The purpose of the research is an ecological and faunal analysis of trematodes in fish inhabiting reservoirs of the European part of Russia.Materials and methods. Helminthological studies were conducted in 23 reservoirs of the European part of Russia from 2011 to 2021. The fish aged two to seven years were analyzed by methods generally accepted in ichthyoparasitology.Results and discussion. Twelve fish species from families Cyprinidae, Percidae, Esocidae and Odontobutidae were found to be infected with 29 trematode species from 14 genera which were represented by 68.9% of metacercariae. Most trematodes had a wide specificity: Tylodelphys clavata was found in 9 fish species; Diplostomum spathaceum and Paracoenogonimus ovatus in 7 species and Apophallus muehlingi in 6 species. The expanded host range for T. podicipina was observed. A. muehlingi, which is an alien species for the Volga-Caspian fisheries basin was identified in most reservoirs. Trematodes by their prevalence in reservoirs are divided into background (5 species), common (6), rare (8) and very rare (10) trematodes. Trematode fauna of fish in reservoirs included 6 to 16 species. The highest species diversity was detected in the Belgorod (16 species), Yakhroma (13 species), Uglich and Chelnav (12 species), Pestovsk and Pyalovsk (11 species) reservoirs. Fifteen trematode species were found in the perch; 12, in the bream and roach; 9, in the pike perch; 8, in the pike and rudd; 6, in the ruff and white bream; 4, in the Volga pikeperch and crucian carp; 3, in the tench; and 1, in the Amur sleeper. The formed foci of trematode infections were observed, namely, postodiplostomosis and ichthyocotylurosis infection. Three trematode species, Pseudoamphistomum truncatum, A. muehlingi and Rossicotrema donicum, were detected that pose a real and potential danger to human health and warm-blooded animals.
Special features of helminth fauna of Mustelidae in the Polistovsky National Nature Reserve and development factors
Authors: Tsvetkov I. N. , Tsvetkova K. N. , Korablev N. P.
Published: April 2023

The purpose of the research is to study the species composition of helminths of the Mustelidae family taking into account biotopic and trophic factors in the Polistovsky National Nature Reserve.Materials and methods. Faeces of Mustela putorius, Neovison vison, Lutra lutra and Martes martes were studied to determine a helminth fauna composition in the Mustelidae family and the factors affecting its development. Helminth eggs were detected using coproovoscopy. For detection of nematode eggs in the feces, the Forate sugar solution flotation method was used; for detection of trematode and cestode eggs, the successive washing method was used. Sexually mature nematodes of the genus Eucoleus were obtained from the pine marten by the method of B. V. Romashov. Species diagnostics of helminth eggs was carried out according to determinants, monographic and other works. The morphometric study of eggs was carried out using the Screen Meter computer program with an accuracy of 0.001 mm. The final diagnostics was carried out by comparing eggs from the faeces of predators and eggs from mature helminths. To study the trophic factor in helminth fauna development, the faeces composition was studied.Results and discussion. The studied mustelids were infected by nematodes Eucoleus sp., Eucoleus aerophilus and Capillaria putorii, and trematodes Isthmiophora melis and Opisthorchiidae sp. Helminth eggs were found in 42.3% samples. The helminth eggs prevail in the material from the polecat and American mink (60.5%) over that from the otter (30.5%). Stenobionts, stenophagy and morphophysiology determine a low infection rate in the otter versus the mink and polecat. Mixed coniferous-small-leafed forest shows a richer list of helminths than the marsh. The obtained data evidences limited helminth circulation in high moor. It is not possible to differentiate eggs of E. aerophilus and E. trophimenkovi according to the previously proposed shape index due to the high individual variability of eggs. High moor as a specific biotope reduces biodiversity including helminths.
Air pollution during operation of the Gradiška - Banja Luka highway
Authors: Knežević Nebojša , Knežević Danijela
Published: April 2023

Aerozagađenje predstavlja koncentraciju štetnih materija preko tolerantne granice (maksimalno dozvoljene koncentracije – MDK), međutim, ova će koncentracija ovisiti ne samo o intenzitetu izvora zagađivanja, nego i od lokalnih uslova u pogledu zračnih strujanja, padavina, konfiguracije terena itd. Djelovanje aerozagađenja na stanovništvo može biti direktno i indirektno. Aerozagađenje direktno oštećuje i pogoršava zdravlje ljudi, dok se njegovo indirektno djelovanje ispoljava putem štetnog uticaja na domaće životinje, vegetaciju, materijalna i kulturna dobra. Saobraćaj je glavni zagađivač vazduha azotnim oksidom. Takođe, utvrđeno je da saobraćaj značajno učestvuje u zagađenju vazduha i česticama prašine PM10, sumpordioksidom SO2, kao i ugljenmonoksidom CO. U ovom radu su prikazani rezultati mjerenja aerozagađenja koji su osnova za dalju ocjenu kvaliteta vazduha kada je u pitanju ekploatacija autoputa Gradiška – Banja Luka.
Parking issue within the context of achieving energy efficiency in cities
Authors: Gajić Ranka , Batarilo Svetlana , Kordić Milena
Published: April 2023

Within the guidelines and parameters for achieving an energy-efficient urban space, an important thematic subgroup deals with the issue of land use planning in the context of accessibility, where the topic of availability and presence of parking is emphasized as very important. The essence of the concept is the reduction of the use of ones own vehicles and, in connection with that, as the end result, the reduction of the number of available parking spaces. The creation and maintenance of an energy-efficient urban environment requires the realization of a large number of different activities that are impossible to realize simultaneously. (For now) there is no way to establish a hierarchy of realization between all the required activities (the order in which they are carried out), which can lead to big mistakes and problems. The paper describe the results of applying a methodology based on the idea of sustainable development, which could contribute to the systematization of a large number of activities and thus ensure that the concept/idea of reducing the use of own vehicles without lowering the quality of life of citizens is gradually implemented and becomes a reality.
The use of CRISPR/Cas9 gene editing in cancer research and therapy
Authors: malik ahmed
Published: April 2023

The use of CRISPR/Cas9 gene editing in cancer researchand therapy
LUMaR: a sensitive macroinvertebrate index of stream condition combining observed-to-expected ratios and sensitivity weightings
Authors: Walsh Christopher John
Published: April 2023

The usefulness of biological indicators of stream condition depends on their sensitivity to human disturbance, and consistency of response across physiographic gradients. Biotic indices such as SIGNAL can be sensitive to human disturbance, but their maximum value may vary across physiographically diverse regions, limiting regionally consistent assessment of condition. Observed-to-expected (OE) indices overcome this problem, but can be insensitive. The LUMaR index, combining OE ratio and taxon-sensitivity weightings, was developed for the Melbourne region, Australia: expected taxa were determined from 59 macroinvertebrate family-distribution models and sensitivity weightings from the direction and slope of response to human impact variables. Sensitivity of LUMaR was compared with that of biotic and OE indices, based on correlations with forest and urban land cover gradients across 109 sites, and by the difference between 3 reference and 3 degraded sites compared to within-site variance. Of 9 indices tested, LUMaR performed best. It was more strongly independently correlated with human impacts than other indices. Its maximum value was invariant across the region, unlike the biotic indices assessed. Compared to other OE indices, LUMaR’s prediction of impairment was improved by weighting by the expected probability of occurrence and by the incorporation of a negative weighting for unexpected weedy and invasive taxa. LUMaR is a more generally applicable measure of stream condition in the Melbourne region, with greater sensitivity to degradation than currently used macroinvertebrate indices, and should be considered as a primary index for condition reporting in the region. Similar indices could be developed in other regions with sufficient monitoring data to develop underlying models.
Proceding Book The 47th Asia Pacific Academic Consortium for Public Health Conference, October 21-23 2015, “The Size Of The Working Tools, Anthropometry And Musculoskeletal Disorders (Review At The Tailors)”
Authors: Berek Noorce Christiani
Published: April 2023

The tailor is a worker who does in groups or individually working of making garments include the process of measuring body size, making patterns, cutting fabric, sewing and refnement. All the work process likely to cause MSDs because the size of the working process tool that did not correspond to the lenght of the leg so that workers working with bowed and bent in a long time this ultimately leads to muscle pain or MSDs in the worker's body. This tipe of research is an analytic survey research with cross sectional design. The population in this study was all tailor workers who work in Kelurahan Solor, Kupang, especially on the road Udayana as many as 35 tailors. The whole population being sampled in this study. Data were analyzed usingchi squaretest. Reserach findings show that there is a significant realtionship between the size of the working tools of the suitability of anthropometry (p= 0,020) with MSDs. It is then suggested to workers should work with the appropriate office chair with anthropometry workers and do break when already feeling tired or stressed muscles of the body by stretching and relaxation.
Deliberative Democracy, Perspective from Indo-Pacific Blogosphere: A Survey
Authors: Akinnubi Abiola , Agarwal Nitin
Published: April 2023

Deliberation and communication within the national space have had numerous implications on how citizens online and offline perceive government. It has also impacted the relationship between opposition and incumbent governments in the Indo-Pacific region. Authoritarian regimes have historically had control over the dissemination of information, thereby controlling power and limiting challenges from citizens who are not comfortable with the status quo. Social media and blogs have allowed citizens of these countries to find a way to communicate, and the exchange of information continues to rise. The quest by both authoritarian and democratic regimes to control or influence the discussion in the public sphere has given rise to concepts like cybertroopers, congressional bloggers, and commentator bloggers, among others. Cybertroopers have become the de facto online soldiers of authoritarian regimes who must embrace democracy. While commentator and congressional bloggers have acted with different strategies, commentator bloggers educate online citizens with knowledgeable information to influence the citizens. Congressional bloggers are political officeholders who use blogging to communicate their positions on ongoing national issues. Therefore, this work has explored various concepts synonymous with the Indo-Pacific public sphere and how it shapes elections and democracy.
Balancing the bio in a biopsychosocial model of hazardous drinking and alcohol use disorders
Authors: Morris James , Boness Cassandra L. , Burton Robyn , Dar-Nimrod Ilan , Moss Antony
Published: April 2023

In their recent primer (Hazardous drinking and alcohol use disorders. Nat. Rev. Dis. Prim. 2022 81 8, 1–25 (2022))1, MacKillop and colleagues present an extensive account of hazardous drinking and alcohol use disorder (AUD), purporting to take a biopsychosocial evaluation of causes and treatment. Whilst in agreement with much of the evidence presented, on close reading we wish to challenge the heavy focus on biological factors, particularly concerning the role of genetics. In response, we wish to highlight several additional concepts and issues concerning the aetiology, prevention and treatment of hazardous drinking and AUD.
SK Dekan No. 44/SK/2012
Authors: SIANTURI HERY LEO
Published: April 2023

Dalam rangka meningkatkan mutu pelayanan dosen khususnya Tridarma perguruan tinggi diperlukan pengakuan kinerja sehingga dosen yang bersangkutan dapat meningkatkan kinerjanya
“近自杀”现象：生命与道德之集体主义困境
Authors: 阮明皇  , 靳蕊宁
Published: April 2023

在经典小说《罪与罚》中，费奥多尔·陀思妥耶夫斯基艺术地描述了罗迪翁·罗曼诺维奇·拉斯科尔尼科夫的精神痛苦和道德困境。他是一个年轻的知识分子，也是具有同情心的前法学院学生，生活在圣彼得堡的贫民窟[1]。拉斯科尔尼科夫的内心在利他主义犯罪行为和道德底线之间徘徊。当他看到身边人们的悲惨生活时，他会用功利地利他主义理由来为自己的罪行辩护[2]：“为什么不杀掉一个悲惨、贪婪、‘无用’的老放贷人，并用这些钱来减轻其他人的痛苦？”有了这个想法，拉斯科尔尼科夫决定犯罪，但很快他开始被罪恶感所折磨。这部小说已经出版了一个多世纪，然而还有许多人仍然面临着拉斯科尔尼科夫所面临的精神痛苦和道德困扰。
SK Dekan No. 11/SK/2014
Authors: SIANTURI HERY LEO
Published: April 2023

Dalam rangka meningkatkan mutu pelayanan dosen khususnya Tridarma perguruan tinggi diperlukan pengakuan kinerja sehingga dosen yang bersangkutan dapat meningkatkan kinerjanya.