bluuebunny/crossref_metadata_2025 huggingface dataset:
The influence of temperature, light, pH and salinity on germination and growth of Cutandia memphitica (Spreng.) Benth
Authors: Faraj Ammar H. , Colak Ender S , Isik Dogan
Published: August 2024

Background: There are many weed species that affect yield and quality in date palm orchards. Cutandia memphitica (Spreng.) Benth. is one of the weeds that is common in date palm orchards in Iraq. However, there is not enough information about C. memphitica in the literature. In order to provide information about the effect of key environmental factors (temperature, light, pH, and salt) on the seed biology of this weed species, this study was conducted.Methods: Within 2020, seeds were collected from agricultural regions in southern Iraq, and trials were done at Erciyes University Faculty of Agriculture labs in Kayseri Türkiye. All experiments were carried out with 30 seeds in the sterile 90 mm (90×15) petri dishes containing two layers of sterile filter paper.Results: The results of this investigation revealed that the seeds of this species germinated by 72.49% in three days when incubated at 25°C, and by 96.66% in 14 days when incubated at 15°C. The results also indicated that even during the longest incubation time examined, which was 28 days, the seeds of this species never germinated at 30 degrees or above. They can germinate during this period if kept at 5°C. The study also revealed that 90% of the seeds of this species germinated in complete darkness, whereas only 11.66% germinated in light. However, light has a significant impact on studied growth parameters (plant height and seedling fresh and dry weight). The results also demonstrate that the pH levels examined, which varied from 4 to 10, had no effect on the seed germination and growth parameters of seedling.Conclusion: The results showed that the seeds of this species germinated at the highest salinity levels (250 mM NaCl) by 10.83%. Meanwhile, the best germination was recorded at 25 mM salinity level of 89.16%, but without significant differences with salinity level at 50 mM and control. Also, the growth indicators were reduced significantly at the salinity level of 250 mM compared to the control.Keywords: Light; Salinity; Seed Germination; Temperature; Weed; Date Palm
Construction of subunit-based DNA vaccines against tuberculosis using five different Mycobacterium specific genes
Authors: Zaman Muhammad Mohsin , Shahzad Mirza Imran , Jilani Aeman , Yousaf Areeba , Bashir Ansah , Riaz Saher , Rivera Gildardo
Published: August 2024

Background: Tuberculosis remains the major infectious and contagious disease with respect to morbidity and mortality around the globe. The main etiological agent of TB is Mycobacterium tuberculosis (M. tb). The cure and/or control of this disease is getting difficult day by day especially due to emergence of drug resistant strains, HIV co-infection and unavailability of good vaccines. BCG is the only available and permitted vaccine against TB, which has variable efficacy only limited to childhood. Therefore, new, well defined, antigen specific and state of the art Molecular Biology based DNA vaccines are required.Methods: The current study is designed to develop subunit-based DNA vaccines against tuberculosis by using five different Mycobacterium specific genes, namely Rv1908c/KatG, Rv0350/DnaK, Rv0440/GroEL2, Rv0934/PstS and Rv3418c/GroES. All the selected genes were amplified through PCR and cloned into mammalian expression pVAX1 vector.Results: All the transformed constructs were confirmed through restriction digestion, colony PCR and sequence analysis. These constructs will be used for various in Vivo immunization studies and also for the challenge studies against TB in future experiments.Conclusion: DNA vaccines, alone or in combination with BCG, have enough potential to be a good therapeutic tool for TB and reduce the treatment time in future.Keywords: DNA vaccine; pVAX1; Tuberculosis; Vaccines
Extraction and Partial Purification of Glucoamylase from Rhizopus oryzae by Solid State Fermentation using Agro-Industrial Residues
Authors: Issa Qays Majeed
Published: August 2024

Background: Filamentous fungi are used to produce many industrial enzymes because these fungi have a great ability to produce such enzymes, especially when using solid substrates that are usually low in cost. The widespread use of this enzyme has made it very important to work on improving its production to achieve the maximum possible benefit.Methods: Five fungal isolates were obtained from soil and decaying fruits and vegetables. All isolates were identified as Rhizopus spp. and cultured on Potato Dextrose Agar (PDA) plates escorted by starch 1%. The appearance of clearance zones around the fungal colonies represents the ability of fungi to produce Glucoamylase. Different solid substrates, nitrogen sources, and temperatures were used to improve enzyme production.Results: Wheat bran gave the highest enzyme production with specific activity 6.25 U/mg, and yeast extract was the potent inducer for enzyme production with specific activity 9.28 U/mg. The optimal temperature for enzyme production was 30°C with specific activity10.27U/mg. Maximum specific activity 18.40U/mg was recorded at 55% saturation with ammonium sulfate precipitation. The result showed increased specific activity 24.84U/mg with dialysis. Partial purification of the enzyme revealed that pH5 was optimum for enzyme-specific activity 9.2U/ml; higher enzyme-specific activity was found with MnSO4 (11.83U/ml) as the best enzyme inducer.Conclusion: This investigation attempted to examine R. oryzae as an effective maker of GA. Innovative work into the nearby creation of GA for industrial use utilizing local resources has demonstrated financial effectiveness. It requires improvement into consistency as needed by worldwide associations dealing with industrial enzymes. Keywords: Glucoamylase; Production; Partial purification; Rhizopus oryzae; Solid state fermentation
Exploring Gene Polymorphisms Associated with Otitis Media through Variable Number Tandem Repeats (VNTR) Region
Authors: Khalil Safa Amer , Hasan Kawther M. A.
Published: August 2024

Background: Otitis is an inflammation and infection of the inner lining of the middle ear. The problem known as suppurative otitis media if there are droplets and holes in the tympanic membrane due to this inflammation and infection of the mucous membrane. The present study focused on specific genes suspected to be associated with otitis media in 60 subjects clinically diagnosed with OM The study focuses on two genes, MUC5B and IL-1RN, whose genes mutations by variable number of tandem repeat (VNTR) regionsMethods: Variable numbers of tandem repeats (VNTR) regions were used to genotype 60 patients with otitis media and 30 healthy individuals for genotypic polymorphisms for the MUC5B and IL-1RN genes by number of tandem repeats (VNTR) regions on a variable basis Blood samples were collected from participants, and three milliliters were placed directly into EDTA tubes. Subsequently, PCR products were detected by agarose gel electrophoresis and visualized by ethidium bromide staining. The size of amplified DNA fragments was determined by comparison with a 100-bp DNA ladder molecule size markerResults: About the same, the paper represents the association of gene polymorphisms with otitis media by genotyping a total of 60 patients as OM, and, on the other hand, 30 individuals were noted to be healthy. This was to determine genotypic polymorphisms for the two genes, that is, MUC5B and IL1RN, within the VNTR region. The results showed that the carriers of allele 2 in the genotypic position had a risk of nearly twofold getting otitis media.Conclusion: Polymorphism of the MUC5B and IL-1RN genes can be responsible for the severity of the inflammatory process in the middle ear, complicated by otitis media or hearing loss.Keywords: Otitis media; Gene polymorphism; MUC5B; IL-1RN; VNTR
Effect of Supportive Care on Quality of Life for People Living with Human Immunodeficiency Virus: A Systematic Review
Authors: Tahir Muh. Yusuf , Malik Muh. Zuki , Wijaya I Kade , Kadar Kusrini S , Halim Mubin Risna , Hamid Firdaus
Published: August 2024

The survival of people living with HIV (PLWH) affects physical, psychological, sexual, social, and financial circumstances during the care and treatment phase. Supportive care is an effective intervention associated with a better quality of life. The aim of this systematic study was to identify effect supportive care intervention on quality-of-life infected people. This study was a systematic review to examine the effect of supportive care in PLWH. Four databases including Science Direct, ProQuest, PubMed, and Wiley library were included to identify the relevant articles. A total of eight articles out of the 247 publications potentially met the inclusion criteria. Supportive care was grouped into four categories, including Community Based Intervention, M-Health Intervention, Social Media-Based Interventions as well as Psychosocial Interventions. The combination of all interventions showed that supportive care needs to be prescribed in line with the conditions and needs of PLWH. Conclusion, several supportive care approaches including Group Support Psychotherapy (GSP), Peer Support (PS), Run4Love WeChat, ALWH treatment strategy, effective communication, and psychosocial assessment, Mental health care pathway PASEO and combination logotherapy, ACT, as well as family psychoeducation (FPE) affected the quality of life for PLWH. This helped in characterizing the intervention and its effect on the conditions of HIV patients.Keywords: Databases; Supportive care; Psychosocial interventions; Quality of life
Chronic sleep deprivation, noise and high fat diet markedly induce C-reactive protein level in mice model
Authors: Attiyah Sama
Published: August 2024

Background: C-reactive protein (CRP) is a circulating sign of systemic chronic inflammation that your liver produces if your body is inflamed. Stressful events over a long period of time are linked to inflammatory disorders. Stress pathways are triggered by noise and sleep deprivation, as well as unhealthy habits like fatty meals. Several studies have connected higher CRP levels with plasma levels. The current study aims to explore the potential association’s relationship between chronic sleep deprivation, noise, high fat diet, and CRP disorders.Methods: Three studies were carried out in our study to evaluate C-reactive protein levels in male Swiss albino mice groups after exposure to three different physical stressors for four weeks. The first study mice group were exposed to chronic sleep deprivation; second group was exposed to electrostatic speaker noise stressors; and the third group was fed a high fat diet. Quantitative estimation of C – reactive protein level in plasma using an ELISA kit.Results: Significant changes in inflammatory CRP levels in the examined plasma were detected in the sleep deprivation group. CRP levels were found to be steadily increasing after two weeks, reaching a significant peak in the fourth week (p < 0.001). Moreover, in the fourth week, there were substantial increases in CRP plasma level with independent evidence, in the experimental noise group (p < 0.001). As well, the biomarker of inflammation level increased across the dietary high density fat diet, reaching the highest level at the end of the study (p < 0.05).Conclusion: Our findings contribute to the body of evidence indicating a link between external stress and subclinical inflammatory markers. Furthermore, CRP concentrations are triggered by chronic sleep deprivation and noise exposure, and their concentrations rise with prolonged dietary fatty acid consumption.Keywords: Sleep deprivation; Noise; High fat diet; Inflammation; CRP
Exploring Listeria monocytogenes in Ewe Milk: ssrA Gene-based Real-time PCR Identification, Phylogenetic Analysis, and Antibacterial Assessment of Magnesium Oxide Nanoparticles Synthesized with Myrtus communis Leaf Extract
Authors: Sadeq Jenan Nadhim , Mizher Balsam Miri , Alsaedi Alyaa Abdulhussein , Khudhair Ola Hakim
Published: August 2024

Background: Listeria monocytogenes, a zoonotic pathogen affecting humans and animals, exhibits a global distribution, including Iraq. This study focused on the rapid identification and phylogenetic analysis of L. monocytogenes in freshly collected ewe milk samples from various farms in Al-Qadisiyah province, Iraq.Methods: The study was conducted with care and precision, involving 150 milk samples. These samples were subjected to traditional bacterial isolation and identification using the enrichment culture method and biochemical tests, with the PCR technique confirming the results. The antibacterial activity of MgONPs was then assessed using the disc diffusion method, ensuring a comprehensive and reliable approach to the study.Result: The results show that 150 ewe milk samples underwent real-time PCR (RT-PCR) targeting the ssrA gene, followed by partial 16S rRNA gene sequencing (PSGS) of purified conventional PCR products. Furthermore, the study entails the biosynthesis of magnesium oxide nanoparticles using Myrtus communis  leaf extract, followed by a comprehensive characterization utilizing UV-spectra, FTIR, SEM, and TEM techniques. The Agar well diffusion method assessed the antibacterial efficacy of these Biosynthesized nanoparticles against L. monocytogenes. The RT-PCR results revealed the presence of L. monocytogenes in 36 out of 150 samples (24%). Subsequent PCR analysis confirmed the presence of the pathogen in 30 out of these 36 positive samples (83.33%). Sequencing of two purified PCR products demonstrated 100% nucleotide identity with global isolates from Iraq and Turkey. Furthermore, the study demonstrated that L. monocytogenes exhibited substantial sensitivity (24.66 ± 0.3) to the biosynthesized magnesium oxide nanoparticles. These findings underscore the speed and precision of the RT-PCR method for detecting L. monocytogenes in fresh ewe milk samples.Conclusion: This comprehensive investigation enhances our understanding of L. monocytogenes prevalence in ewe milk and highlights the potential of Myrtus communis -derived nanoparticles for combating this pathogen.Keywords: Antibacterial nanoparticles; Listeria monocytogenes; Magnesium oxide; Myrtus communis  sheep milk; ssrA gene
Evaluation of BACTEC™ FX and BacT/Alert™ Automated Blood Culture Systems for Detection of Clinically Relevant Bacterial and Yeast Species
Authors: Absar Muhammad , Alshahrani Ibraheem , Nashmy Fahd , Salami Mosa , Hadadi Ali , AlMarzooq Aous , AlAsiri Alhussain , AlRashidi Abdulaziz , AlFaifi Mohammed
Published: August 2024

Background: Bloodstream infections (BSI) are a significant health concern, necessitating effective diagnostic tools. This study evaluates the performance of the BD Bactec FX system in comparison to the established BacT/Alert system, examining mean time till detection (TTD) across various blood culture bottles.Methods: The study involves 148 blood culture bottles, inoculated with representative bacterial ATCC strains (n= 15) and 04 yeast isolates. Parallel testing is conducted by seeding 10–30 colony-forming units (CFU) in duplicate in both BD Bactec FX and BacT/Alert systems. TTD is assessed across aerobic, anaerobic, and pediatric bottles for diverse microbial species.Results: All 148 bottles tested in parallel show positive signals in both systems. BD Bactec FX demonstrates significantly shorter TTD for adult-seeded cultures in aerobic and anaerobic bottles compared to BacT/Alert Similarly, pediatric bottles with BD Bactec FX exhibit a shorter TTD compared to BacT/Alert. The statistical significance of TTD, 95% confidence intervals (CI), and p-values is evident for Bactec FX aerobic, anaerobic, and pediatric bottles across tested organisms. Notable examples of faster TTD include; Bacteroides ovatus (16.6h by Bactec FX vs. 70.5h by Bact/Alert 3D), Stenotrophomonas maltophilia (33.7h vs. 72.5h), and Streptococcus pyogenes (8.1h vs. 11.9h).Conclusions: In conclusion, the study’s findings demonstrate that BD Bactec FX surpasses BacT/Alert in prompt microbial detection, showcasing potential for early identification of bacteremia and fungemia. Faster TTD implies the potential to initiate timely antimicrobial treatment, thereby reducing patient morbidity and mortality. However, exceptions in certain microbial species highlight the need for comprehensive clinical validation to establish the broad applicability of these findings.Keywords: Blood Culture System; Recovery Rate; Time to detection; Blood stream infection; BTA3D; Bactec FX
First Report on Clinical Feasibility of Dried Blood Spot Technique for Hemoglobin Estimation in Cholistani Cattle
Authors: Chaudhary Maryam , Farooq Umer , Idris Musadiq , Lashari Mushtaq Hussain , Qasim Saira , Afzal Muhammad Abrar , Khan Musarat Abbas , Ali Asad
Published: August 2024

Background: The dried blood spot (DBS) technique using filter papers has revolutionized the conventional blood sampling techniques through ease of blood collection, storage and transport. Various analytes (such as hormones, antigens, antibodies and hematochemical attributes) are being estimated through DBS globally. However, this technique has not yet been implied in Pakistan. This research work is the first of its kind regarding hemoglobin (Hb) estimation in Cholistani cattle (n=63) blood through DBS technique using filter paper.Methods: Three methods of Hb estimation were implied in the present study viz. through veterinary hematology analyzer (HbA), and two indirect cyanmethemoglobin methods (HbIC and HbICX) using measured (20µL) and unmeasured blood drops on the filter paper, respectively.Results: Results revealed that HbA and HbIC were non-significantly (P≥0.05) different from each other, being different from HbICX (P≤0.05). The HbICX gave overestimated values of Hb as compared to HbA and HbIC. The sensitivity, specificity, positive predictive value, and negative predictive value for HbIC were higher being 86.1%, 88.3%, 88.0%, and 86.0%, respectively as compared to the lower values of 45.0%, 12.0%, 12.0% and 45.0%, for HbICX. Bland and Altman test revealed a better level of agreement between HbA and HbIC. Around the mean difference line, there was no proportional bias in data distribution (Mean= -0.16, 95% CI= 0.34 to -0.67). Similarly, measures attained through Cronbach alpha and intraclass correlation coefficient between HbA and HbIC were higher being 0.703 and 0.825 values for single and average, respectively, as compared to the values of 0.200 and 0.333 between HbA and HbICX.Conclusion: It is concluded that the indirect cyanmethemoglobin method for Hb estimation is reliable and accurate for cattle blood, if a measured quantity of blood drop is taken on a filter paper. We recommend this DBS technique for Hb estimation in cattle blood for resource-poor settings and for livestock herds being reared distantly from the laboratories. For future, it is recommended that DBS technique with various other modifications and for other hematochemical biomarkers may be validated for livestock blood.Keywords: Dried blood spot; Hemoglobin; Cholistani cattle
Exploring Natural Compounds as Promising Matrix Metalloproteinase-2 inhibitors for Cancer Management: A Biocomputational Study
Authors: Aloqbi Akram Ahmed
Published: August 2024

Background: Matrix metalloproteinase-2 (MMP2) plays a role in breaking down the components of the extracellular matrix, which allows cancer cells to advance and invade. Therefore, the inhibition of MMP2 shows potential as a promising strategy for treating cancer.Methods: This study employed computational screening to identify MMP2 inhibitors from a collection of 2,405 natural compounds. GLXC-26716, the co-crystal ligand of MMP2, served as the positive control. Virtual screening was performed using PyRx 8.0 software to find molecules that might inhibit the active site of MMP2.Results: The virtual screening process has identified five potential candidates: ZINC000000001412, ZINC000001612328, ZINC000001614079, ZINC000000119988, and ZINC0000000047553. These candidates were selected based on their strong binding affinities and interactions with MMP2. These compounds, which adhere to Lipinski's Rule of Five and have significant physicochemical properties, show promise as MMP2 inhibitors.Conclusion: The finding of this study indicates a preliminary investigation into an innovative approach for managing cancer that inhibits the invasion and dissemination of cancer cells.Keywords: Matrix Metalloproteinase-2; Natural compounds; Cancer; Virtual screening
Identification of phytochemicals as potential inhibitors of Candida albicans SAP3: An in-silico approach
Authors: Alhindi Zain
Published: August 2024

Background: Candida albicans is a major threat to human health, causing a variety of diseases. Candida albicans can overgrow and trigger infections like thrush, diaper rash, and vaginal yeast infections. Therefore, finding potential inhibitors of C. albicans from plant sources has become important, recently.Methods: This study investigated the interaction of natural compounds (N = 450) from the ZINC database with the C. albicans SAP3 protein. PyRx tools were used to conduct molecular docking analyses, which included ligand and target receptor preparation, data analysis, and visualization.Result: The binding energies between the natural compounds and SAP3 ranged from -8.8 to -7.8 kcal/mol, indicating that the interactions were predominantly strong. Among the compounds screened, ZINC000252509014, ZINC000252509722, ZINC000253389136, ZINC000251981944, and ZINC000252480871 were extensively explored in this study because they were found to be the best among others. Furthermore, these compounds showed favorable drug-like properties.Conclusion: These findings lay the groundwork for future research aimed at developing potent SAP3 inhibitors to treat C. albicans infections.Keywords: Candida albicans; SAP3 protein; Natural compounds; Virtual screening; Drug-likeness
Application of the PCR for diagnosis and monitoring of rotavirus in calves during treatment with the antiviral agent Triazavirin
Authors: Reshetnikova Tatiana , Kuzmin Vladimir , Krylova Tatyana
Published: August 2024

Background: Rotavirus is a significant cause of morbidity and mortality in neonatal calves, leading to severe gastrointestinal and systemic illnesses. Current treatments primarily focus on symptomatic relief and prevention through vaccines, with limited use of antiviral agents. This article  considers the issue of experiments using the antiviral agent Triazavirin in the treatment of rotavirus infection in calves, with tracking the amount of virus during the polymerase chain reaction examination in fecal samples.Methods: The experiment was conducted at a Mordovian Republican Veterinary Laboratory in the National Research Mordovian State University named after N.P. Ogarev (Republic of Mordovia, Russia), between February and March 2023. Two groups of calves, experimental and control, were assessed. The experimental group received Triazavirin orally at a dose of 500 mg per day for 7 days. Before and after the experiment, fecal samples were taken from experimental calves for the PCR study of the ribonucleic acid of group A rotaviruses.Results: Treatment with Triazavirin resulted in a reduction of the rotavirus load by an average of 35.2%. The PCR analyses before and after treatment indicated a substantial decrease in viral RNA in the majority of treated calves, with variable individual responses.Conclusions: Triazavirin demonstrates potential as an effective antiviral treatment for rotavirus in calves, reducing viral load and possibly improving clinical outcomes. The use of PCR provided a reliable method for monitoring viral levels, thereby facilitating effective management of this infectious disease.Keywords: Calves; Treatment; Digestive and Respiratory Organs; Antiviral Agent; Rotavirus; Treatment
Exploring the Sequence analysis of the Two-Component System Response Regulator OmpR in Multi-Drug Resistant (MDR) Acinetobacter baumannii
Authors: Radwan Ali Israa , Abdulsatar Duaa Mohammed , Salman Ruqaia Sabbar , Khudhair Anfal Mohammed
Published: August 2024

Background: The study focuses on the clinical profiles, antibiotic susceptibility, and genetic characteristics of 25 A. baumannii isolates from patients with urinary tract infections (UTIs). Patient Cohort: Ages range from 18 to 70 years, with a male to female ratio of 1.375. Notable Finding: Some multidrug-resistant (MDR) isolates had commonalities with extensively drug-resistant (XDR) variations, showing the evolution of drug resistance.Methods: Antibiotic Efficacy Analysis: Evaluation of antibiotic efficacy when the variable moved from lower (Bin 1) to higher (Bin 2) levels. Correlation Matrix: An analysis of antibiotic correlations to better identify potential cross-resistance and shared characteristics. Genetic Diversity Analysis: An examination of variants in the OmpR gene, including mutations and polymorphisms. Sequence analysis is used to identify point mutations in OmpR, with an emphasis on transitions such as adenine (A) to guanine (G).Results: Significant improvement in antibiotic efficacy from Bin 1 to Bin 2. Correlation Findings: Antibiotics have complex interactions, which may indicate cross-resistance. Genetic diversity: Variations in the OmpR gene have implications for virulence and adaptability. Sequence Analysis: The majority of point mutations in OmpR were transitions, with A typically changing to G.Conclusion: In Iraq, researchers have discovered the first evidence that clinical resistance in A. baumannii may be caused by structural alterations in the OmpR gene. A. baumannii isolates' genetic diversity at certain locations suggests possible implications on virulence and adaptation.Keywords: Acinetobacter baumannii; OmpR; Polymorphism; MDR
Comparison of alfentanil and fentanyl in painless colonoscopy in children
Authors: Ningning CUI  , Bo XU  , Shuo LI  , Peng ZHANG  , Guanglin SHANG
Published: August 2024

[Objective] To explore the safety and efficacy of alfentanil and fentanyl combined with propofol in painless colonoscopy in children.
[Methods] 60 children aged 3-7 years old who were going to do colonoscopy in our hospital were randomly divided into alfentanil group (AF group) and fentanyl group (F group), with 30 cases in each group. The dosage of alfentanil 10ug/kg and fentanyl 2ug/kg respectively were used in both groups. The recovery time, induced cough, vital signs, physical activity, drug supplementation, postoperative adverse reactions and doctor satisfaction of the two groups of children were observed and recorded.
[Results] The recovery time of AF group was shorter than that of F group, which was (11.40±4.33) minutes and (9.37±2.81) minutes respectively (P< 0.05). The induced cough in AF group was less than that in F group (P< 0.05). AF group had more body motion than F group (P< 0.05). There were no significant differences in blood pressure, heart rate, oxygen saturation, respiratory rate and other vital signs between the two groups (P> 0.05), but the values of vital signs at all observation points in the two groups were lower than those before anesthesia. No adverse reactions occurred in the two groups, and there was no significant difference in the satisfaction of doctors between the two groups.
[Conclusion] Both alfentanil and fentanyl can be effectively used in painless colonoscopy in children. However, alfentanil is more suitable for painless colonoscopy than fentanyl due to its shorter resuscitation time, lower incidence of induced cough and no increased risk of adverse reactions.
Effects of improved right subclavian vein catheterization on the direction of the catheter tip in children
Authors: Xueqing WANG  , Yuanzhen CHEN  , Jing ZHAO  , Dajun XING  , Bo XU  , Liang XU
Published: August 2024

Background. Catheter tip malposition is commonly observed during subclavian vein catheterization. This study was to investigate whether position and partially retracted the guide wire could reduce catheter malposition.
Methods. The children needing right subclavian venipuncture catheterization were randomly divided into two groups: test group A (82 patients) and control group B (82 patients). In group A, catheterization was performed with the patient in a supine position and the guide wire partially retracted during placement. In contrast, in group B, catheterization was done with the patient in Trendelenburg without partial retraction of the guide wire. The catheter tip direction was examined using intraoperative ultrasound, and a postoperative chest X-ray was obtained.
Results. The rate of correct catheter tip direction at first insertion was higher in group A compared to group B (86.2% vs. 66.7%, P < 0.05). There were no significant differences in incidence and general data between the two groups. The repeated vascular puncture was unsuccessful in three patients (2 cases in group A and 1 case in group B), and right internal jugular vein catheter instead.
Conclusions. The guide wire can be partially retracted in the supine position to reduce catheter tip malposition during right subclavian venipuncture in children.
The Efficacy of Ultrasound-Guided Microwave Ablation for Benign Thyroid Nodules and the Correlation Analysis of Its Influencing Factors
Authors: Zhijin XING  , Xiaohua XU  , Zhiheng HUANG  , Manlin LAI  , Zhenzhou LI
Published: August 2024

Objective: To investigate the efficacy of ultrasound-guided microwave ablation (MWA) for benign thyroid nodules and to analyze the correlation between treatment outcomes and various influencing factors.
Methods: 95 patients with pathologically confirmed benign thyroid nodules（ totally 96 nodules) who underwent MWA under ultrasound guidance were enrolled in this retrospective study. Pre-ablation and post-ablation follow-up examinations including two-dimensional ultrasound, color Doppler, and contrast-enhanced ultrasound were conducted at 1, 3, and 6 months. The clinical efficacy was assessed, including the reduction in nodule volume and the success rate of ablation. Factors that might influence treatment outcomes, such as patient age, gender, nodule size, type, and ablation parameters, were analyzed. Statistical correlations were evaluated using Spearman’s correlation coefficient and multiple regression analysis.
Results: MWA has demonstrated favorable efficacy in treating benign thyroid nodules, with a low incidence of complications. Most nodules experienced significant volume reduction or even complete disappearance after treatment (P < 0.05). The average volume reduction ratio (VRR) of benign thyroid nodules undergoing MWA at 1 month, 3 months, and 6 months post-operation were 52.98%±22.23%, 68.18%±19.81%, and 82.39%±15.03%, respectively, with a significant reduction rate (VRR > 50%) observed in 95.8% (92/96) of the nodules (VRR > 50%). Cystic nodules exhibited the most substantial volume reduction following ablation. The initial volume of the nodule, blood flow status, nodule composition, and the energy absorption per unit volume were all related to the postoperative VRR (P < 0.05). Factors such as patient age, gender, and ablation duration did not show significant correlation with ablation efficacy (P＞0.05).
Conclusion: MWA is a promising minimally invasive treatment for benign thyroid nodules. The treatment efficacy is closely related to the initial volume of the thyroid nodule, the composition of the nodule, the blood flow situation of the nodule, and the ablation energy per unit volume.
Application Value of Competing Risk Models in the Prevention of Preeclampsia
Authors: Hang GAO  , Kai YAN  , Ruxiu GE  , Zhifeng MO
Published: August 2024

Preeclampsia is a common complication during pregnancy that severely impacts both maternal and fetal health. In recent years, competing risk models have emerged as a novel statistical method increasingly used to investigate the risk factors and prevention strategies associated with preeclampsia. This review summarizes the current applications of competing risk models in preeclampsia research, emphasizing their value in risk assessment, preventive interventions, and clinical decision-making. Additionally, it highlights future research directions that could further enhance the understanding and management of this condition.
2024年港美通科技（深圳）有限公司教育部产学合作协同育人项目申报指南
Authors: 港美通科技（深圳）有限公司
Published: August 2024

2024年，港美通科技（深圳）有限公司拟在“四新”领域建设、教学内容和课程体系改革、师资培训、实践条件和实践基地建设、创新创业教育改革等方面开展产学合作协同育人项目，计划立项35项，支持高校的人才培养和专业综合改革。
消防工程专业课程思政教学改革及实践——以“消防燃烧学”为例
Authors: 闫琪  , 郭子东  , 郑兰芳  , 王霁  , 李伟东
Published: August 2024

为贯彻思想政治育人理念，实现立德树人根本任务，全国高校思想政治工作会议上明确指出，把社会主义核心价值观的培育和实践等思想政治工作融入教学教育全过程，实现全员、全程和全方位育人。课程思政的提出为新时代消防工程专业课程教学改革及实践提出新的要求，“消防燃烧学”是消防工程专业的核心课程，对学生的专业基础理论知识和实践能力培养具有重要作用。在此背景下，“消防燃烧学”课程以提高学生的实际应用能力和综合素质为目标，从课程思政、教学模式、教学设计和综合性评价方式等多个环节进行课程思政教学改革，深挖课程思政教育资源，制定了具体实施方案。实践证明，课程思政和教学改革并举，在增强学生对消防工程专业的价值认同、社会服务意识、激发学生学习动力等方面，取得较好的教学效果。本文提出的教学研究可为消防工程专业课程思政改革及实践提供经验和指导。
德国“双元制”模式及其实施的必要条件研究
Authors: 刘璐
Published: August 2024

本文探讨了德国双元制职业教育模式的特点及其成功实施的必要条件，并通过潍坊科技学院的具体案例展示了如何有效地实施这一模式。首先介绍了德国双元制模式的基本概念和基本思想，接着深入分析了双元制模式的主要过程，包括学校教学、企业实训以及交替进行的模式，并阐述了其本质特征，即实践导向、个性化发展和终身学习的文化。同时，本文还详细阐述了双元制模式的内涵，包括全面发展、社会责任感的培养和适应能力的提升，并概述了成功实施该模式所需的政策支持、企业参与、完善的教育体系以及社会认可等必要条件。最后，通过潍坊科技学院的具体案例，展示了如何通过校企合作、优质实训基地建设和PDCA管理模式的应用来有效实施双元制模式，为学生提供更好的职业发展机会。
产教融合背景下C语言程序设计混合式教学模式探究
Authors: 李睿智  , 何锦涛  , 刘贺  , 王齐  , 刘尚穹
Published: August 2024

随着信息技术的迅速发展和产业需求的变化，传统的课堂教学已经无法满足学生对编程能力的需求。本研究旨在探究产教融合背景下，C语言程序设计课程的混合式教学模式，以提高学生的编程能力和实践能力。本文结合C语言程序设计课程的特点和教学目标，设计了相应的混合式教学方案，包括线上学习平台搭建、课堂教学内容设计和实践项目安排等。在实施阶段，本研究通过实地调研和学生反馈，对混合式教学模式进行了评估和改进。结果显示，采用混合式教学模式能够有效地提升学生的学习积极性和编程能力，增强了学生的实践能力和问题解决能力。
PENDAMPINGAN BELAJAR SISWA USIA SEKOLAH DASAR DI PADUKUHAN KAYEN MELALUI PROGRAM MERDEKA BELAJAR KAMPUS MERDEKA
Authors: Susanti Susanti , Fahmi Muhamad , Himawati Fety , Nurrohman Sidiq , Lestari Tata Sri , Kurnia Siti Aulia , Maulidah Sinta Dewi , Fifuadi Shulhi , Makhfudah Rizki , Julmiati Julmiati , Utami Selly , Farhana Zada , Darojatun Khoirunnas , Miyono Ahmad Kurnia , Ariyani Yusinta Dwi , Wahyudi Andi
Published: August 2024

One of the problems experienced by the Padukuhan Kayen community is the difficulty of elementary school students in participating in online learning, so that learning assistance is needed for children in elementary school. These problems include not understanding the material, not doing assignments straightforwardly, to the role of parents who are not right in helping students learn at home. The purpose of this activity is to provide learning assistance to students to train academic abilities and master learning objectives, starting from tutoring reading and numeracy, doing assignments, and providing explanations of school materials that are not yet understood.The stages of mentoring carried out include the preparation stage, the socialization stage, and the implementation stage of activities. The location of the mentoring is planned in Kayen Hamlet, Kalurahan Sendangsari, Pajangan, Bantul. The results obtained by students are being able to read and count well, being more active in doing schoolwork, and better understanding learning materials at school.This activity received a very good response from local residents. The children are also very enthusiastic in participating in activities as evidenced by always being present when there is a schedule of guidance and trying to complete the tasks given. At the end of the activity, KKN-T students held a children's quiz competition as an evaluation of the implementation of the activity.
TINGKAT PARTISIPASI MASYARAKAT PADA PROGRAM VAKSINASI COVID-19 DI DESA PENDARUNGAN KECAMATAN KABAT
Authors: Mubarok Zaki Al , As’adi Moh.
Published: August 2024

This study aims to determine the level of community participation and the inhibiting factors in the Covid-19 vaccination program in Pendarungan Village, Kabat District. The type of research used in this research is quantitative research with a descriptive approach. The research location in this study is Pendarungan Village, Kabat District, Banyuwangi Regency. This research involved 4 respondents and 2 informants consisting of village officials and local communities. Data collection techniques used are observation, interview, and documentation techniques. While the data analysis used in this study includes data reduction, data presentation, drawing conclusions, and levers. The results showed that the level of community participation was still relatively low, this was due to the lack of knowledge and education provided by the government about the importance of vaccination. One of the inhibiting factors is the availability of misinformation from social media regarding the effects received by the public after being vaccinated. This is one of the important things so that the government always preserves all information that is wrong, inappropriate or hoax.
PSIKOEDUKASI PENDIDIKAN SEKS KEPADA GURU DAN SISWA SEBAGAI UPAYA PENCEGAHAN KEKERASAN SEKSUAL PADA SISWA SD
Authors: Masruroh Fitriatul , Putri Ellyana Ilsan Eka , Hidayah Fathi , Faishol Riza
Published: August 2024

Community service on the socialization of sex education to teachers and students to improve understanding and skills related to sexual violence in children as an effort to prevent sexual violence against children. This service is also motivated by the results of cases of sexual abuse in children which are increasing every year so that prevention is needed through psychoeducation of sex in children. The method used in this service is mentoring and training which consists of three stages, namely preparation, implementation and monev. After this service program, the ability of teachers and students to demonstrate that sex education can increase teacher and student understanding of sexual violence.
MITIGASI RESIKO USAHA PAKAIAN BEKAS
Authors: Rini Puspa , Siregar B. Wisman S. , Yulianto Kampono I. , Freddy John
Published: August 2024

Business and selling are alternative solutions for alumni of the Tahfidz Islamic Boarding School, one of which is a recycled clothing fashion brand which can be done without a long process. In addition to the variations of the models that are very diverse and in accordance with the desires of young people's self-confidence, used clothes are relatively affordable. In fact, due to its "affordability", many teenagers are interested in not only using it as buyers but sellers. Problems then arise, including aspects of legality, halalness, hygiene, mitigating the risk of purchasing in a closed bale system. The purpose of this service is to provide counseling on mitigating the risks of trying used clothes if the scale of purchase (for resellers) is in the "wholesale/bal" system (closed packaging whose condition cannot be checked first). With this counseling, sellers can have comprehensive insight into risk mitigation: selling risk mitigation, mitigating the risk of justification for society, halal-haram, legal/illegal, to the most important being able to accurately calculate forecasts for wholesale purchases or the contents of 'bals' very tightly closed. This is a business mitigation, if the calculation is wrong, it will certainly have a fatal impact, namely no return of capital, aka loss. The solution delivery technique is counseling. Counseling is intended to broaden the horizons of students in anticipating all the risks of trying used clothes. The result of the service activity is that the high risk mitigation of used clothes is the amount of damaged goods which is very high risk according to the term buying cats in sacks. For students who are going to buy retail or items that are already on display, it is more recommended because they can be checked before buying. As for purchases by wholesale in closed bales, including subhat (very doubtful), this has invited a lot of debate, but the servant's recommendation is to buy by "head quantity", ie opening one bale with certainty of buying 10 to 25 units of goods.
KEGIATAN MENGAJAR, NON MENGAJAR DAN ADMINISTRASI SEKOLAH DALAM MBKM ASISTENSI MENGAJAR DI PANGKALPINANG
Authors: Nurmaisi Nurmaisi , Hidayatulah Ilham Azis , Vebtasvili Vebtasvili
Published: August 2024

Asistensi mengajar merupakan salah satu bentuk pelaksanaan program Merdeka Belajar - Kampus Merdeka (MBKM) yang bertujuan untuk memberikan kesempatan bagi mahasiswa yang memiliki minat dalam bidang pendidikan untuk turut serta mengajarkan dan memperdalam ilmunya ; membantu meningkatkan pemerataan kualitas pendidikan ; serta relevansi pendidikan dasar dan menengah dengan pendidikan tinggi dan perkembangan zaman. Kegiatan asistensi mengajar ini telah dilaksanakan selama 16 minggu di SMP Muhammadiyah Pangkalpinang, Provinsi Kepulauan Bangka Belitung, yang dilakukan mahasiswa secara kolaboratif di bawah bimbingan guru pamong dan dosen fasilitator. Dalam kegiatan ini, mahasiswa melakukan praktik mengajar khususnya dalam mata pelajaran matematika, pendidikan kewarganegaraan, dan akidah akhlak. Mahasiswa juga melakukan kegiatan non mengajar seperti membantu pelaksanaan kegiatan-kegiatan di sekolah, mendampingi siswa dalam kegiatan karnaval dan jalan sehat, membantu reparasi bangunan sekolah dan penataan perpustakaan. Pada bagian administrasi, mahasiswa membantu guru menyiapkan perangkat pembelajaran dan bahan ajar, serta membantu pendataan kunjungan dan peminjaman buku di perpustakaan. Melalui kegiatan ini, mahasiswa dapat meningkatkan softskills dan hardskills seperti kepemimpinan, inovasi atau pemecahan masalah langsung dari lapangan, kemampuan komunikasi, dan kemampuan di bidang desain. Sedangkan bagi sekolah mitra kegiatan ini mampu meningkatkan pelayanan pendidikan baik dari sisi pengajaran ataupun non pengajaran dan administrasi.
PENGOLAHAN BUAH SUKUN MENJADI NUGGET BERNILAI EKONOMI DI SMP 10 BANTIMURUNG KABUPATEN MAROS
Authors: Arif Arwin
Published: August 2024

Kegiatan pengabdian pada masyarakat ini dilakukan pada guru-guru SMP 10 Bantimurung Kabupaten Maros. Adapun tujuan yang ingin dicapai dalam pengabdian kepada masyarakat ini adalah (1) mengubah mindset guru-guru sehingga berkeinginan memanfaatkan dan membudidayakan buah sukun, (2) melatih guru-guru dalam mendiversifikasi buah sukun menjadi produk yang bernilai ekonomi. Pelaksanaan pengabdian kepada masyarakat ini menggunakan 3 (tiga) metode yaitu (1) metode parsitipasi aktif , (2) metode diversifikasi produk dan (3) metode pengemasan dan labelling .Hasil kegiatan pengabdian dari evaluasi penilaian kepuasan guru-guru dalam mengikuti kegiatan sosialisasi berupa penjelasan mengenai pengolahan buah sukun sebesar 79,14%. Hal ini menandakan guru- guru berparsitipasi aktif dan mampu memotivasi jiwa wirausaha mereka dalam mengolah buah sukun menjadi lahan bisnis. Selain itu, evaluasi pengolahan buah sukun menjadi nugget yaitu 61,91 %. Hal ini berarti, guru-guru sudah memiliki motivasi dalam kategori sedang ketika ingin melakukan pengolahan buah sukun menjadi nugget.
SOSIALISASI PENCATATAN DAN PENYUSUNAN LAPORAN KEUANGAN SEDERHANA BAGI PELAKU UMKM
Authors: Fitriany Finka , Damayanty Prisila , Wildayani Aulia , Istinganah Ibnatul , Nuradilah Khoiriyah , Rahmadi Zara Tania
Published: August 2024

This program is carried out with the aim of providing education about recording and preparing financial statements in a simple and easy-to-understand way for UMKM actors, in this case the Ersha Parfum Store. The nature of this community service program is discussion-based socialization / counseling with the aim that the material we convey at the Ersha Parfum Store can be easily understood and answer the problems they experience in recording transactions in the business. The results of the community service program show that the Ersha Parfum Store is interested in slowly changing the way their transactions are recorded in the way we conveyed, because according to them, even with a slightly more recording flow, it is quite easy to read the business situation that occurs more easily. This shows that this service program is enough to increase their insight and interest in the structure of recording and preparing their financial statements.
PENGENALAN AKUNTANSI DASAR UNTUK UMKM
Authors: Astiningsih Della Rohma Dwi , Damayanty Prisila , Davita Enza , Sobari Fajar , Leliyana Leliyana , Setiawan Indra
Published: August 2024

MSME in Indonesia are one of the pillars of the Indonesian economy apart from cooperatives. The development of the number of MSME has increased from year to year, but only a few MSME have experienced developments in terms of their financial performance. This is because MSME still have weak knowledge of the process of accounting records and low awareness of the importance of accounting information in world business. Therefore, community service activities in the form of simple accounting introduction training for MSME are still very much needed. This training is intended for SMEs in Jagakarsa. Implementation of activities carried out by using the method of lectures, tutorials, and discussions. The results of this activity indicate that SMEs in Jagakarsa in running their business tend not to practice accounting. Some MSME actors in Jagakarsa also don't understand accounting and what the purpose of making financial reports is. This community service activity helps provide advice, motivation and knowledge to all MSME in Jagakarsa to implement simple training practices in their business activities so that they can improve their financial business performance
PENYULUHAN PRA NIKAH DALAM PERSPEKTIF ISLAM: Upaya Meningkatkan Pengetahuan Tentang Pernikahan Pada Daerah Rawan Perceraian
Authors: Mustahal Muhamad
Published: August 2024

Pengabdian ini bertujuan untuk meningkatkan pemahaman tentang pernikahan pada daerah rawan perceraian. Perceraian disebabkan oleh Minimnya pengetahuan berkeluarga baik dalam persiapan menikah menjadi salah satu kendala yang sering dihadapi oleh calon pasangan suami istri, bahkan dialami juga oleh pasangan yang telah menikah. Di sisi lain pelaksanaan pendidikan pra nikah di Indonesia masih sangat terbatas,biasanya dilakukan oleh Kementerian Agama tiap Kabupaten, Kantor Urusan Agama (KUA), dan Badan Penasihatan Pembinaan dan Pelestarian Perkawinan (BP4) di Kecamatan. Lokasi Pengabdian ini berada di kecamatan Bruno Kabupaten Purworejo. Pendekatan yang dilakukan dalam melaksanakan kegiatan ini menggunakan Participatory Action Research (PAR). Hasil dari kegiatan ini adalah 1) Faktor Tingginya Tingkat Perceraian Di Kecamatan Bruno Kabupaten Purworejo dikarenakan; Pendidikan yang rendah, pernikahan dini yaitu di bawah batas usia pernikahan, Putus Sekolah. Pelaksanaan Penyuluhan Pra Nikah Dalam Perspektif Islam Sebagai Upaya Meningkatkan Pengetahuan Tentang Pernikahan Pada Daerah Rawan Perceraian Di Kecamatan Bruno Kabupaten Purworejo berjalan dengan baik acara dimulai dengan Pre Test dan penyampaian materi oleh 2 dua Narasumber dan diakhiri dengan Post Test. Hasilnya terdapat peningkatan pemahaman Pra Nikah Perspektif Islam.
Educação integral: o que é possível por meio do Programa Mais Educação?
Authors: Gama Maria Eliza Rosa , Sônego Fabricia
Published: August 2024

Este artigo é um recorte de uma pesquisa de mestrado cujo foco abordou as formas de organização do trabalho escolar para implementação de uma proposta de educação integral. Teve abordagem qualitativa, as fontes de informação foram os docentes e os instrumentos de coleta, as entrevistas e observações. Durante a pesquisa elencamos os aspectos favoráveis e os limites encontrados na organização do trabalho escolar em relação aos documentos escolares e as legislações acerca da educação integral.
Objeto de aprendizagem como ferramenta na produção textual
Authors: Almeida Lia Heberlê de , Lemos Ana Paula Rodrigues Kuhls , Porto Neuza Correa
Published: August 2024

Este artigo se refere a um estudo no 5° ano do ensino fundamental em uma escola municipal, com o objetivo de verificar a contribuição do uso de objetos de aprendizagem na produção textual. Realizado com a intenção estimular a produção de textos através de objetos de aprendizagem. Os alunos foram avaliados pela participação e produção usando o OA, em comparação com a produção textual em sala de aula. Verificou-se que, apesar de algumas dificuldades, os textos foram mais criativos e variados.