bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Association Study Reveals Host Factors Affecting Conjugation in Escherichia coli
Authors: Van Wonterghem Laetitia , De Chiara Matteo , Liti Gianni , Warringer Jonas , Farewell Anne , Verstraeten Natalie , Michiels Jan
Published: March 2022

The emergence and dissemination of antibiotic resistance threaten the treatment of common bacterial infections. Resistance genes are often encoded on conjugative elements, which can be horizontally transferred to diverse bacteria. In order to delay conjugative transfer of resistance genes, more information is needed on the genetic determinants promoting conjugation. Here, we focus on which bacterial host factors in the donor assist transfer of conjugative plasmids. We introduced the broad-host-range plasmid pKJK10 into a diverse collection of 113 Escherichia coli strains and measured by flow cytometry how effectively each strain transfers its plasmid to a fixed E. coli recipient. Differences in conjugation efficiency of up to 2.7 and 3.8 orders of magnitude were observed after mating for 24 h and 48 h, respectively. These differences were linked to the underlying donor strain genetic variants in genome-wide association studies, thereby identifying candidate genes involved in conjugation. We confirmed the role of fliF, fliK, kefB and ucpA in the donor ability of conjugative elements by validating defects in the conjugation efficiency of the corresponding lab strain single-gene deletion mutants. Based on the known cellular functions of these genes, we suggest that the motility and the energy supply, the intracellular pH or salinity of the donor affect the efficiency of plasmid transfer. Overall, this work advances the search for targets for the development of conjugation inhibitors, which can be administered alongside antibiotics to more effectively treat bacterial infections.
Bacterial Biosorbents, an Efficient Heavy Metals Green Clean-Up Strategy: Prospects, Challenges, and Opportunities
Authors: Pham Van Hong Thi , Kim Jaisoo , Chang Soonwoong , Chung Woojin
Published: March 2022

Rapid industrialization has led to the pollution of soil and water by various types of contaminants. Heavy metals (HMs) are considered the most reactive toxic contaminants, even at low concentrations, which cause health problems through accumulation in the food chain and water. Remediation using conventional methods, including physical and chemical techniques, is a costly treatment process and generates toxic by-products, which may negatively affect the surrounding environment. Therefore, biosorption has attracted significant research interest in the recent decades. In contrast to existing methods, bacterial biomass offers a potential alternative for recovering toxic/persistent HMs from the environment through different mechanisms for metal ion uptake. This review provides an outlook of the advantages and disadvantages of the current bioremediation technologies and describes bacterial groups, especially extremophiles with biosorbent potential for heavy metal removal with relevant examples and perspectives.
Synthesis of Organic Iodine Compounds in Sweetcorn under the Influence of Exogenous Foliar Application of Iodine and Vanadium
Authors: Grzanka Marlena , Smoleń Sylwester , Skoczylas Łukasz , Grzanka Dominik
Published: March 2022

A human’s diet should be diverse and rich in vitamins, macro- and microelements essential for the proper functioning of the human body. Globally, a high percentage of the human population suffers from malnutrition, deficiencies of nutrients and vitamins also known as the problem of hidden hunger. This problem it is not only common in poor countries, but also occurs in developed countries. Iodine is a nutrient crucial for the proper functioning of the human and animal body. For plants, it is referred to as a beneficial element or even a microelement. The design of the biofortification experiment was determined on the basis of the interaction of iodine and vanadium (synergistic interaction in marine algae), where vanadium-dependent iodoperoxidase catalyzes apoplastic oxidation of iodine, resulting in high efficiency of iodine uptake and accumulation in brown algae (Laminaria digitate). Three independent experiments (Exp.) were carried out with the foliar application of vanadium (V) and iodine (I) compounds. The main differences between the experiments with the adapted proper corn biofortification method were the different application stage between the individual experiments, the application intervals and the dose of the iodine–vanadium compound. In each experiment, the accumulation of iodine and vanadium in the grain was several times lower than in the leaves. The combination iodine and vanadium significantly increased the accumulation of iodine in the grain in the case of applying V with inorganic iodine compounds, and a decrease in the accumulation of I after applying V with organic iodine compound —especially in Exp. No. 3. In grain, the highest content of I−, IO3− was in combination with the application of 2-iodobenzoic acid (products of its metabolism). In most of the tested combinations, vanadium stimulated the accumulation/synthesis of exogenous/endogenous 5-iodosalicylic acid (5ISA) and 2-iodobenzoic acid (2IBeA), respectively, and decreased the content of 2,3,5-triiodobenzoic acid (2,3,5-triIBeA) in leaves and grains. The tested compounds I and V and the combinations of their application had a diversified effect on the vitamin C content in the grains. Vanadium in the lower dose of 0.1 µM significantly increased the sugar content in the grain.
The Production and Evaluation of an Electrochemical Sensors for Strychnine and Its Main Metabolite Strychnine N-Oxide for Their Use in Biological Samples
Authors: Qader Bakhtiyar , Hussain Issam , Baron Mark , Estevez-Brito Rafael , Cassella John Paul , Gonzalez-Rodriguez Jose
Published: March 2022

Strychnine (STN) and its major metabolite Strychnine N-Oxide (SNO) were examined electrochemically. Both parent compounds and its major metabolite showed electroactivity on glassy carbon electrodes using CV and DPV techniques. One oxidation peak at 1008 mV was observed for STN with the optimum peak intensity at pH 7. SNO produced two oxidation peaks, at 617 mV and 797 mV, at pH 5. The peaks demonstrated irreversible behaviour and the irreversibility of the system was confirmed at different scan rates. A calibration curve was produced for both CV and DPV measurements and the sensitivity of the proposed EC method was good compared with previous electrochemical and non-electrochemical methods. The precision of oxidation peak of STN using the STN-MIP method produced a maximum value of 11.5% and 2.32% for inter-day and intraday %RSD, respectively. The average% recovery was around 92%. The electrochemical method has been successfully applied to the determination of STN in spiked plasma and urine samples. For SNO, both anodic peaks of SNO demonstrated irreversible behaviour. A different sweep rate was used for calculating the number of ‘transfer electrons’ in the system; based on this, the mechanism of oxidation reaction was proposed. Calibration curves for both oxidative peaks were produced using DPV measurements. The second anodic peak demonstrated high linearity and precision with %RSD < 1.96%.
Synthesis, Characterization, and Assessment of Anti-Cancer Potential of ZnO Nanoparticles in an In Vitro Model of Breast Cancer
Authors: Aljabali Alaa A. A. , Obeid Mohammad A. , Bakshi Hamid A. , Alshaer Walhan , Ennab Raed M. , Al-Trad Bahaa , Al Khateeb Wesam , Al-Batayneh Khalid M. , Al-Kadash Abdulfattah , Alsotari Shrouq , Nsairat Hamdi , Tambuwala Murtaza M.
Published: March 2022

Advanced innovations for combating variants of aggressive breast cancer and overcoming drug resistance are desired. In cancer treatment, ZnO nanoparticles (NPs) have the capacity to specifically and compellingly activate apoptosis of cancer cells. There is also a pressing need to develop innovative anti-cancer therapeutics, and recent research suggests that ZnO nanoparticles hold great potential. Here, the in vitro chemical effectiveness of ZnO NPs has been tested. Zinc oxide (ZnO) nanoparticles were synthesized using Citrullus colocynthis (L.) Schrad by green methods approach. The generated ZnO was observed to have a hexagonal wurtzite crystal arrangement. The generated nanomaterials were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), UV-visible spectroscopy. The crystallinity of ZnO was reported to be in the range 50–60 nm. The NPs morphology showed a strong absorbance at 374 nm with an estimated gap band of 3.20 eV to 3.32 eV. Microscopy analysis proved the morphology and distribution of the generated nanoparticles to be around 50 nm, with the elemental studies showing the elemental composition of ZnO and further confirming the purity of ZnO NPs. The cytotoxic effect of ZnO NPs was evaluated against wild-type and doxorubicin-resistant MCF-7 and MDA-MB-231 breast cancer cell lines. The results showed the ability of ZnO NPs to inhibit the prefoliation of MCF-7 and MDA-MB-231 prefoliation through the induction of apoptosis without significant differences in both wild-type and resistance to doxorubicin.
Unveiling the Effect of Solvents on Crystallization and Morphology of 2D Perovskite in Solvent-Assisted Method
Authors: Su Yingjie , Xue Jianqiang , Liu Anmin , Ma Tingli , Gao Liguo
Published: March 2022

Controlling the crystallographic orientations of 2D perovskite is regarded as an effective way to improve the efficiency of PSCs based on 2D perovskite. In this paper, five different assistant solvents were selected to unveil the effect of solvents on crystallization and morphology of 2D perovskite in a solvent-assisted method. Results demonstrated that the effect of Lewis basicity on the crystallization process was the most important factor for preparing 2D perovskite. The stability of the intermediate, reacted between the solvent and the Pb2+, determined the quality of 2D film. The stronger the Lewis basicity was, the more obvious the accurate control effect on the top-down crystallization process of 2D perovskite would be. This could enhance the crystallographic orientation of 2D perovskite. The effect of Lewis basicity played a more important role than other properties of the solvent, such as boiling point and polarity.
Critical Computational Evidence Regarding the Long-Standing Controversy over the Main Electrophilic Species in Hypochlorous Acid Solution
Authors: Chen Ke-Wei , Wu Yun-Dong , Sun Tian-Yu
Published: March 2022

Although hypochlorous acid (HOCl) solution has become a popular electrophilic reagent for industrial uses, the question of which molecule (HOCl or Cl2) undergoes electrophilic addition with olefins remains a controversial issue in some literature and textbooks, and this problem has been largely underexplored in theoretical studies. In this work, we computationally studied the electrophilic addition mechanism of olefins using three experimentally predicted effective electrophilic chlorinating agents, i.e., HOCl, Cl2, and Cl2O molecules. Our results demonstrate that Cl2 and Cl2O are the main electrophilic agents in HOCl solution, whereas the HOCl molecule cannot be the electrophile since the energy barrier when directly adding HOCl molecule to olefins is too high to overcome and the “anti-Markovnikov” regioselectivity for tri-substituted olefin is not consistent with experiments. Notably, the HOCl molecule prefers to form oxonium ion intermediate with a double bond, rather than the generally believed chlorium ion intermediate. This work could benefit mechanistic studies of critical biological and chemical processes with HOCl solution and may be used to update textbooks.
Isolation and Characterization of a ssDNA Aptamer against Major Soluble Antigen of Renibacterium salmoninarum
Authors: Layman Brady , Mandella Brian , Carter Jessica , Breen Haley , Rinehart John , Cavinato Anna
Published: March 2022

Bacterial kidney disease (BKD) is a major health problem of salmonids, affecting both wild and cultured salmon. The disease is caused by Renibacterium salmoninarum (Rs), a fastidious, slow-growing and strongly Gram-positive diplobacillus that produces chronic, systemic infection characterized by granulomatous lesions in the kidney and other organs, often resulting in death. Fast detection of the pathogen is important to limit the spread of the disease, particularly in hatcheries or aquaculture facilities. Aptamers are increasingly replacing conventional antibodies as platforms for the development of rapid diagnostic tools. In this work, we describe the first instance of isolating and characterizing a ssDNA aptamer that binds with high affinity to p57 or major soluble antigen (MSA), the principal antigen found on the cell wall surface of Rs. Specifically, in this study a construct of the full-length protein containing a DNA binding domain (MSA-R2c) was utilized as target. Aptamers were isolated from a pool of random sequences using GO-SELEX (graphene oxide-systematic evolution of ligands by exponential enrichment) protocol. The selection generated multiple aptamers with conserved motifs in the random region. One aptamer with high frequency of occurrence in different clones was characterized and found to display a strong binding affinity to MSA-R2c with a Kd of 3.0 ± 0.6 nM. The aptamer could be potentially utilized for the future development of a sensor for rapid and onsite detection of Rs in water or in infected salmonids, replacing time-consuming and costly lab analyses.
Phytochemical Insights into Ficus sur Extracts and Their Biological Activity
Authors: Sieniawska Elwira , Świątek Łukasz , Sinan Kouadio Ibrahime , Zengin Gokhan , Boguszewska Anastazja , Polz-Dacewicz Małgorzata , Bibi Sadeer Nabeelah , Etienne Ouattara Katinan , Mahomoodally Mohamad Fawzi
Published: March 2022

This study focused on the biological evaluation and chemical characterisation of Ficus sur Forssk. (F. sur) (Family: Moraceae). The methanolic and aqueous extracts’ phytochemical profile, antioxidant, and enzyme inhibitory properties were investigated. The aqueous stem bark extract yielded the highest phenolic content (115.51 ± 1.60 mg gallic acid equivalent/g extract), while the methanolic leaves extract possessed the highest flavonoid content (27.47 ± 0.28 mg Rutin equivalent/g extract). In total, 118 compounds were identified in the tested extracts. The methanolic stem bark extract exhibited the most potent radical scavenging potential against 2,2-diphenyl-1 picrylhydrazyl and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (475.79 ± 6.83 and 804.31 ± 4.52 mg Trolox equivalent/g extract, respectively) and the highest reducing Cu2+ capacity (937.86 ± 14.44 mg Trolox equivalent/g extract). The methanolic stem bark extract substantially depressed tyrosinase (69.84 ± 0.35 mg kojic acid equivalent/g extract), α-amylase (0.77 ± 0.01 mmol acarbose equivalent/g extract), acetylcholinesterase and butyrylcholinesterase (2.91 ± 0.07 and 6.56 ± 0.34 mg galantamine equivalent/g extract, respectively) enzymes. F. sur extracts were tested for anticancer properties and antiviral activity towards human herpes virus type 1 (HHV-1). Stem bark infusion and methanolic extract showed antineoplastic activity against cervical adenocarcinoma and colon cancer cell lines, whereas leaf methanolic extract exerted moderate antiviral activity towards HHV-1. This investigation yielded important scientific data on F. sur which might be used to generate innovative phytopharmaceuticals.
Structural, Magnetic, and AC Measurements of Nanoferrites/Graphene Composites
Authors: Habib Shaimaa A. , Saafan Samia A. , Meaz Talaat M. , Darwish Moustafa A. , Zhou Di , Khandaker Mayeen U. , Islam Mohammad A. , Mohafez Hamidreza , Trukhanov Alex V. , Trukhanov Sergei V. , Omar Maha K.
Published: March 2022

As a contribution to the graphene-based nanoferrite composites, this article is intended to present Mn, Co, and Co-Mn nanoferrites for the preparation and investigation of such samples. Nanoparticles of Co ferrite, Mn ferrite, and Co-Mn ferrite were chemically synthesized by the coprecipitation method. The composites of ferrite/graphene were made by incorporating weight ratios of 25% graphene to 75% ferrite. Various structural and characterizing investigations of ferrite samples and ferrite/graphene composites were performed, including XRD, EDX, SEM, VSM hysteresis loops, AC conductivity, and dielectric behavior. The investigations ensured the formation of the intended nanoferrite powders, each having a single-phase crystal structure with no undesired phases or elements. All samples exhibit a soft magnetic behavior. They show a semiconducting behavior of AC electrical conductivity as well. This was proved by the temperature dependence of the AC’s electrical conductivity. Whereas the dielectric function and loss tangent show an expected, well-explained behavior, the ferrite/graphene composite samples have lower saturation magnetization values, lower AC conductivity, and dielectric constant values than the pure ferrites but still have the same behavior trends as those of the pure ferrites. The values obtained may represent steps on developing new materials for expected applications, such as manufacturing supercapacitors and/or improved battery electrodes.
The Effects of Graphene-Family Nanomaterials on Plant Growth: A Review
Authors: Zhang Xiao , Cao Huifen , Wang Haiyan , Zhao Jianguo , Gao Kun , Qiao Jun , Li Jingwei , Ge Sai
Published: March 2022

Numerous reports of graphene-family nanomaterials (GFNs) promoting plant growth have opened up a wide range of promising potential applications in agroforestry. However, several toxicity studies have raised growing concerns about the biosafety of GFNs. Although these studies have provided clues about the role of GFNs from different perspectives (such as plant physiology, biochemistry, cytology, and molecular biology), the mechanisms by which GFNs affect plant growth remain poorly understood. In particular, a systematic collection of data regarding differentially expressed genes in response to GFN treatment has not been conducted. We summarize here the fate and biological effects of GFNs in plants. We propose that soil environments may be conducive to the positive effects of GFNs but may be detrimental to the absorption of GFNs. Alterations in plant physiology, biochemistry, cytological structure, and gene expression in response to GFN treatment are discussed. Coincidentally, many changes from the morphological to biochemical scales, which are caused by GFNs treatment, such as affecting root growth, disrupting cell membrane structure, and altering antioxidant systems and hormone concentrations, can all be mapped to gene expression level. This review provides a comprehensive understanding of the effects of GFNs on plant growth to promote their safe and efficient use.
Experimental Evaluation of Radiation Response and Thermal Properties of NPs-Loaded Tissues-Mimicking Phantoms
Authors: Asadi Somayeh , Korganbayev Sanzhar , Xu Wujun , Mapanao Ana Katrina , Voliani Valerio , Lehto Vesa-Pekka , Saccomandi Paola
Published: March 2022

Many efforts have recently concentrated on constructing and developing nanoparticles (NPs) as promising thermal agent for optical hyperthermia and photothermal therapy. However, thermal energy transfer in biological tissue is a complex process involving different mechanisms such as conduction, convection, radiation. Therefore, having information about thermal properties of tissue especially when NPs are embedded in is a necessity for predicting the heat transfer during hyperthermia. In this work, the thermal properties of solid phantom based on agar in the presence of three different nanoparticles (BPSi, tNAs, GNRs) and alone were measured and reported as a function of temperature (ranging from 22 to 62 °C). The thermal response of these NPs to an 808 nm laser beam with three different powers were studied in the water comparatively. Agar and tNAs have almost constant thermal properties in the considered range. Among the three NPs, gold has the highest conductivity and diffusivity. At 62 °C BPSi NPs have the similar amount of increase for the diffusivity. The thermal parameters reported in this paper can be useful for the mathematical modeling. Irradiation of the NPs-loaded water phantom displayed the highest radiosensitivity of gold among the three mentioned NPs. However, for the higher power of irradiation, BPSi and tNAs NPs showed the increased absorption of heat during shorter time and the increased temperature gradient slope for the initial 15 s after the irradiation started. The three NPs showed different thermal and irradiation response behavior; however, this comparison study notes the worth of having information about thermal parameters of NPs-loaded tissue for pre-clinical planning.
The Changing Landscape of Nutrition in Cystic Fibrosis: The Emergence of Overweight and Obesity
Authors: Bailey Julianna , Krick Stefanie , Fontaine Kevin R.
Published: March 2022

Cystic fibrosis has historically been characterized by malnutrition, and nutrition strategies have placed emphasis on weight gain due to its association with better pulmonary outcomes. As treatment for this disease has significantly improved, longevity has increased and overweight and obesity have emerged issues in this population. The effect of excess weight and adiposity on CF clinical outcomes is unknown but may produce similar health consequences and obesity-related diseases as those observed in the general population. This review examines the prevalence of overweight and obesity in CF, the medical and psychological impact, as well as the existing evidence for treatment in the general population and how this may be applied to people with CF. Clinicians should partner with individuals with CF and their families to provide a personalized, interdisciplinary approach that includes dietary modification, physical activity, and behavioral intervention. Additional research is needed to identify the optimal strategies for preventing and addressing overweight and obesity in CF.
Drug Sensitivity of Vaccine-Derived Rubella Viruses and Quasispecies Evolution in Granulomatous Lesions of Two Ataxia-Telangiectasia Patients Treated with Nitazoxanide
Authors: Faisthalab Raeesa , Suppiah Suganthi , Dorsey Morna , Sullivan Kathleen E. , Icenogle Joseph , Perelygina Ludmila
Published: March 2022

A strong association between rubella virus (RuV) and chronic granulomas, in individuals with inborn errors of immunity, has been recently established. Both the RA27/3 vaccine and wild-type RuV strains were highly sensitive to a broad-spectrum antiviral drug, nitazoxanide (NTZ), in vitro. However, NTZ treatment, used as a salvage therapy, resulted in little or no improvements of RuV-associated cutaneous granulomas in patients. Here, we report investigations of possible causes of treatment failures in two ataxia-telangiectasia patients. Although a reduction in RuV RNA in skin lesions was detected by real-time RT-PCR, live immunodeficiency-related vaccine-derived rubella viruses (iVDRV) were recovered from granulomas, before and after the treatments. Tizoxanide, an active NTZ metabolite, inhibited replications of all iVDRVs in cultured A549 cells, but the 50% and 90% inhibitory concentrations were 10–40 times higher than those for the RA27/3 strain. There were no substantial differences in iVDRV sensitivities, neither before nor after treatments. Analysis of quasispecies in the E1 gene, a suspected NTZ target, showed no effect of NTZ treatments on quasispecies’ complexity in lesions. Thus, failures of NTZ therapies were likely due to low sensitivities of iVDRVs to the drug, and not related to the emergence of resistance, following long-term NTZ treatments.
Performance of Five Metagenomic Classifiers for Virus Pathogen Detection Using Respiratory Samples from a Clinical Cohort
Authors: Carbo Ellen , Sidorov Igor , van Rijn-Klink Anneloes , Pappas Nikos , van Boheemen Sander , Mei Hailiang , Hiemstra Pieter , Eagan Tomas , Claas Eric , Kroes Aloys , de Vries Jutte
Published: March 2022

Viral metagenomics is increasingly applied in clinical diagnostic settings for detection of pathogenic viruses. While several benchmarking studies have been published on the use of metagenomic classifiers for abundance and diversity profiling of bacterial populations, studies on the comparative performance of the classifiers for virus pathogen detection are scarce. In this study, metagenomic data sets (n = 88) from a clinical cohort of patients with respiratory complaints were used for comparison of the performance of five taxonomic classifiers: Centrifuge, Clark, Kaiju, Kraken2, and Genome Detective. A total of 1144 positive and negative PCR results for a total of 13 respiratory viruses were used as gold standard. Sensitivity and specificity of these classifiers ranged from 83 to 100% and 90 to 99%, respectively, and was dependent on the classification level and data pre-processing. Exclusion of human reads generally resulted in increased specificity. Normalization of read counts for genome length resulted in a minor effect on overall performance, however it negatively affected the detection of targets with read counts around detection level. Correlation of sequence read counts with PCR Ct-values varied per classifier, data pre-processing (R2 range 15.1–63.4%), and per virus, with outliers up to 3 log10 reads magnitude beyond the predicted read count for viruses with high sequence diversity. In this benchmarking study, sensitivity and specificity were within the ranges of use for diagnostic practice when the cut-off for defining a positive result was considered per classifier.
Electrostatic Dust Cloth: A Useful Passive Sampling Method When Assessing Exposure to Fungi Demonstrated in Studies Developed in Portugal (2018–2021)
Authors: Viegas Carla , Dias Marta , Viegas Susana
Published: March 2022

Electrostatic dust cloths (EDC) have been widely used for microbiologic contamination assessment in different indoor and occupational environments. This paper reviews sixteen studies performed in Portugal between 2018 and 2021 for evaluating the exposure to microbiological agents and focusing on fungi using EDC as a passive sampling method. The findings suggest that EDC can be applied as a screening method for particulate matter-exposure assessment and as a complementary method to characterize microbial exposures in occupational environments. Overall, EDC should be included, side by side with other sampling methods, in sampling campaigns focused on exposure assessments due to the advantages such as the straightforward extraction protocol favoring the employment of different assays, which allows us to assess exposure to a wide range of microbial agents, and presents higher accuracy regarding the fungal diversity.
Antibiofilm Activity of β-Lactam/β-Lactamase Inhibitor Combination against Multidrug-Resistant Salmonella Typhimurium
Authors: Laure Nana Nguefang , Ahn Juhee
Published: March 2022

This study was designed to assess the effect of β-lactam/β-lactamase inhibitor combinations on the inhibition of biofilm formation of Salmonella Typhimurium. The anti-planktonic and anti-biofilm activities of ampicillin (AMP), ceftriaxone (CEF), and combination treatments of antibiotics and sulbactam (AMP + SUL and CEF + SUL) were evaluated against antibiotic-sensitive S. Typhimurium ATCC 19585 (STAS) and clinically isolated multidrug-resistant (MDR) S. Typhimurium CCARM 8009 (STMDR). Compared to the control, the minimum inhibitory concentrations (MICs) of AMP against STAS and CEF against STMDR were decreased from 32 to 16 μg/mL and 0.25 to 0.125 μg/mL, respectively, in the presence of SUL. The numbers of STMDR treated with AMP + SUL and CEF + SUL were effectively reduced by more than 2 logs after 4 h of incubation at 37 °C. The β-lactamase activities of STAS and STMDR treated with AMP and CEF were reduced from 3.3 to 2.6 μmol/min/mL and from 8.3 to 3.4 μmol/min/mL, respectively, in the presence of SUL. The biofilm cell numbers of STAS and STMDR were reduced at all treatments after 24 h of incubation at 37 °C. The biofilm cell numbers of STAS and STMDR were reduced by more than 2 logs in the presence of SUL compared to the AMP and CEF alone. The lowest relative fitness level was 0.6 in STAS treated with AMP + SUL, while no significant differences in the relative fitness were observed in STMDR. This study suggests that β-lactamase inhibitors (BLIs) could be used for controlling biofilm formation of β-lactamase-producing multidrug-resistant S. Typhimurium.
De Novo Mutation in KMT2C Manifesting as Kleefstra Syndrome 2: Case Report and Literature Review
Authors: Siano Maria Anna , De Maggio Ilaria , Petillo Roberta , Cocciadiferro Dario , Agolini Emanuele , Majolo Massimo , Novelli Antonio , Della Monica Matteo , Piscopo Carmelo
Published: March 2022

Diagnosis of pediatric intellectual disability (ID) can be difficult because it is due to a vast number of established and novel causes. Here, we described a full-term female infant affected by Kleefstra syndrome-2 presenting with neurodevelopmental disorder, a history of hypotonia and minor face anomalies. A systematic literature review was also performed. The patient was a 6-year-old Caucasian female. In the family history there was no intellectual disability or genetic conditions. Auxological parameters at birth were adequate for gestational age. Clinical evaluation at 6 months revealed hypotonia and, successively, delay in the acquisition of the stages of psychomotor development. Auditory, visual, somatosensory, and motor-evoked potentials were normal. A brain MRI, performed at 9 months, showed minimal gliotic changes in bilateral occipital periventricular white matter. Neuropsychiatric control, performed at 5 years, established a definitive diagnosis of childhood autism and developmental delay. Molecular analysis of the exome revealed a novel KMT2C missense variant: c.9244C > T (p.Pro3082Ser) at a heterozygous state, giving her a diagnosis of Kleefstra syndrome 2. Parents did not show the variant. Literature review (four retrieved eligible studies, 10 patients) showed that all individuals had mild, moderate, or severe ID; language and motor delay; and autism. Short stature, microcephaly, childhood hypotonia and plagiocephaly were also present. Conclusion. Kleefstra syndrome 2 is a difficult diagnosis of a rare condition with a high clinical phenotypic heterogeneity. This study suggests that it must be taken in account in the work-up of an orphan diagnosis of intellectual disability and/or autism spectrum disorder.
Investigation of the Way of Phase Synchronization of a Self-Injected Bunch and an Accelerating Wakefield in Solid-State Plasma
Authors: Maslov Vasyl I. , Bondar Denys S. , Onishchenko Ivan N.
Published: March 2022

The electron acceleration, in a laser wakefield accelerator, controlled through plasma density inhomogeneity is studied on a basis of 2.5-dimensional particle-in-cell simulation. The acceleration requires a concordance of the density scale length and shift of the accelerated electron bunch relative to wake bubble during electron acceleration. This paper considers the excitation of a wakefield in plasma with a density equal to the density of free electrons in metals, solid-state plasma (the original idea of Prof. T. Tajima), in the context of studying the wakefield process. As is known in the wake process, as the wake bubble moves through the plasma, the self-injected electron bunch shifts along the wake bubble. Then, the self-injected bunch falls into the phase of deceleration of the wake wave. In this paper, support of the acceleration process by maintaining the position of the self-injected electron bunch using an inhomogeneous plasma is proposed. It is confirmed that the method of maintaining phase synchronization proposed in the article by using a nonuniform plasma leads to an increase in the accelerating gradient and energy of the accelerated electron bunch in comparison with the case of self-injection and acceleration in a homogeneous plasma.
Design and Analysis of a Single Humidity Sensor Based on TDLAS for Water Vapor and Heavy Oxygen Water Vapor Detection
Authors: Gong Ping , Zhou Jian , Er Zhixuan , Ju Yu , Xie Liang
Published: March 2022

In this paper, a single humidity sensor for water vapor and heavy oxygen water vapor detection is presented. The sensor is based on tunable diode laser absorption spectroscopy (TDLAS) and thus has high sensitivity, good selectivity, and a short response time. A 1372 nm distributed feedback (DFB) diode laser is utilized as the light source, the wavelength tuning range of which covers the absorption lines of water vapor and heavy oxygen water vapor. A Herriott gas cell with 12 m optical length is designed for signal-to-noise ratio (SNR) enhancement. The sensor can distinguish between water vapor and heavy oxygen water vapor effectively. The accuracy of water detection is within ±0.5% RH. The accuracy of heavy oxygen water vapor detection is within ±1.0% RH.
Controlling Dispersion Characteristic of Focused Vortex Beam Generation
Authors: Li Xueshen , Fan Zhigang
Published: March 2022

As an important structured beam, vortex beams have a wide range of applications in many fields. However, conventional vortex beam generators require complex optical systems, and this problem is particularly serious with regards to focused vortex beam generators. The emergence of metasurfaces provides a new idea for solving this problem; however, the accompanying chromatic dispersion limits its practical application. In this paper, we show that the dispersion characteristic of focused vortex beam generators based on metasurfaces can be controlled by simultaneously manipulating the geometric and propagative phases. The simulation results show that the transmission-type focused vortex beam generators exhibit positive dispersion, zero dispersion, and negative dispersion, respectively. This work paves the way for the practical application of focused vortex beam generators.
Enhancing Energy Efficiency of the Doze Mode Mechanism in Ethernet Passive Optical Networks Using Support Vector Regression
Authors: Yang Cheng-Zen , Lotfolahi Mohammad Amin , Hwang I-Shyan , Ab-Rahman Mohammed Syuhaimi , Nikoukar Aliakbar , Liem Andrew Tanny , Ganesan Elaiyasuriyan
Published: March 2022

An Ethernet passive optical network (EPON) is an optical access network that allows a higher data rate with low power consumption. To improve energy savings for an EPON, the sleep and doze modes for the optical network units (ONUs) play a pivotal role. Many prediction schemes have been proposed to control these modes. To increase the prediction accuracy, this study proposes an energy-efficient approach that uses a support vector regression (SVR) model. A dynamic bandwidth allocation (DBA) scheme called SVR-DBA is designed to allocate bandwidth to ONUs more efficiently and fairly. To determine the effectiveness of the proposed scheme, simulations are performed. The simulation results show that the proposed scheme decreases energy consumption for ONUs by up to 47% and fulfills the quality-of-service (QoS) requirements in terms of delay, jitter, and packet loss.
Bioactive Compounds and Antifungal Activity of Leaves and Fruits Methanolic Extracts of Ziziphus spina-christi L.
Authors: El-Shahir Amany A. , El-Wakil Deiaa A. , Abdel Latef Arafat Abdel Hamed , Youssef Nora H.
Published: March 2022

Zizyphus spina-christi L. has antimicrobial properties because of the presence of biologically active compounds. Alternaria is an opportunistic pathogen that causes leaf spots, rots, and blights on a variety of plant parts. This study aimed to reduce the usage of synthetically derived fungicides. Identification of the bioactive components present in leaves and fruits methanolic extracts of Z. spina-christi was performed using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The efficacy of the two methanol extracts was tested against (a) in vitro fungal growth and (b) pathogenicity control on non-wounded and wounded tomato fruits. The results revealed that gallic acid and ellagic acid were the major components in leaves extract while quercetin was the major component in fruits extract. In addition, Phenol, 2,5-bis(1,1-dimethylethyl) (40.24%) and Decane, 2-methyl-(18.53%) were the most abundant components in the leaf extract, and the presence of D-mannonic acid, 2,3,5,6-tetrakis-o-(trimethylsilyl), and γ-lactone (22.72%) were major components in fruits extract. The methanolic extracts of Z. spina-christi leaves and fruits demonstrated significant antifungal activity against the growth of Alternaria alternata, A. citri, and A. radicina with variable inhibition percentages at different concentrations. Pathogenicity was increased when the skin was injured, as expected. Both extracts reduced the percentage of infected fruits.
Thaumatin-like Protein (TLP) Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Expression in Response to Fusarium proliferatum Infection
Authors: Anisimova Olga K. , Kochieva Elena Z. , Shchennikova Anna V. , Filyushin Mikhail A.
Published: March 2022

Plant antifungal proteins include the pathogenesis-related (PR)-5 family of fungi- and other stress-responsive thaumatin-like proteins (TLPs). However, the information on the TLPs of garlic (Allium sativum L.), which is often infected with soil Fusarium fungi, is very limited. In the present study, we identified 32 TLP homologs in the A. sativum cv. Ershuizao genome, which may function in the defense against Fusarium attack. The promoters of A. sativumTLP (AsTLP) genes contained cis-acting elements associated with hormone signaling and response to various types of stress, including those caused by fungal pathogens and their elicitors. The expression of AsTLP genes in Fusarium-resistant and -susceptible garlic cultivars was differently regulated by F. proliferatum infection. Thus, in the roots the mRNA levels of AsTLP7–9 and 21 genes were increased in resistant and decreased in susceptible A. sativum cultivars, suggesting the involvement of these genes in the garlic response to F. proliferatum attack. Our results provide insights into the role of TLPs in garlic and may be useful for breeding programs to increase the resistance of Allium crops to Fusarium infections.
Water Shortage Affects Vegetative and Reproductive Stages of Common Bean (Phaseolus vulgaris) Chilean Landraces, Differentially Impacting Grain Yield Components
Authors: Tapia Gerardo , Méndez José , Inostroza Luis , Lozano Camila
Published: March 2022

Water availability for agricultural use is currently a global problem that worsens with climate change in several regions of the world. Among grain legumes, common bean (Phaseolus vulgaris) is the most cultivated in the worldwide. The Chilean germplasm of common bean is characterized by tolerance to water stress. Here, we analyzed a selection of nine ancient Chilean landraces in regard to their drought tolerance, simulating optimal (OW) and restricted watering (RW) in a Mediterranean environment. Phenological, growth, and yield traits were recorded, and correlation analysis was performed. Accordingly, leaf temperature and osmotic potential were higher under RW, while the leaf chlorophyll content decreased in all landraces. Physiological maturity days and seed-filling days were lower in RW than in OW. This similarly occurred with the grain yield. The % yield reduction was negatively correlated with the % pod reduction and the relative rate of leaf expansion (RLAE) reduction. However, the 100-seed weight value was not significantly modified by water treatment (p > 0.05). For instance, landraces that preferred to fill the grain with a lower rate of leaf expansion showed a lower loss in grain yield under drought conditions. These results suggest that the resource partitioning between growing leaves, flowers, and developing pods in Chilean landraces is variable, affecting the common bean drought tolerance.
Buckwheat and Amaranth as Raw Materials for Brewing, a Review
Authors: Dabija Adriana , Ciocan Marius Eduard , Chetrariu Ancuța , Codină Georgiana Gabriela
Published: March 2022

Globally, beer is considered the most-consumed low-alcohol beverage, it ranks third, after water and tea, in the top sales of these drinks. New types of beer are the result of the influence of several factors, including innovations in science and technology, changing requirements for food consumption of the population, competition between producers, promotion of food for health, flavor, and quality, the limited nature of traditional food resource raw materials, and the interest of producers in reducing production costs. Manufacturers are looking for new solutions for obtaining products that meet the requirements of consumers, authentic products of superior quality, with distinctive taste and aroma. This review proposes the use of two pseudocereals as raw materials in the manufacture of beer: buckwheat and amaranth, focusing on the characteristics that recommend them in this regard. Due to their functional and nutraceutical properties, these pseudocereals can improve the quality of beer—a finished product. Additionally, all types of beer obtained from these pseudocereals are recommended for diets with particular nutritional requirements, especially gluten-free diets. Researchers and producers will continue to improve and optimize the sensory and technological properties of the new types of beer obtained from these pseudocereals.
Leaf Coloration in Acer palmatum Is Associated with a Positive Regulator ApMYB1 with Potential for Breeding Color-Leafed Plants
Authors: Sun Sujing , Zhang Qiang , Yu Yongfan , Feng Jianyuan , Liu Changlai , Yang Jiading
Published: March 2022

Anthocyanin biosynthesis and accumulation is closely associated with tissue/organ coloring in plants. To gain insight into the physiological and molecular mechanisms of leaf coloring in Acer palmatum, a deciduous tree during autumnal senescence, we first investigated concentration dynamics of pigments (i.e., chlorophyll, carotenoid and anthocyanin) in leaves with differential coloring. It was found that compared to green leaves (GN), anthocyanins were accumulated actively in semi-red (SR) and total-red (TR) leaves, accompanied with chlorophyll and carotenoid degradation. Then transcriptional profiling on GN and SR leaves identified thousands of transcripts with differential expression in SR compared to GN leaves. An annotation search showed that the entire flavonoid/anthocyanin biosynthesis pathway from the production of naringenin chalcone to modification of flavonoid backbone was extensively activated at the transcriptional level in SR leaves. Phylogenetic analysis of putative MYB proteins identified ApMYB1 as a putative regulator promoting anthocyanin biosynthesis. Expression of ApMYB1 in leaves was induced by exogenous hormones including abscisic acid. Stable overexpression of ApMYB1 in tobacco resulted in leaves with higher accumulation of anthocyanins. Collectively, our results identified ApMYB1 as a positive regulator associated with leaf coloring in Acer palmatum during autumnal senescence, which may be regarded a potential target for breeding color-leafed plants.
Quality Assessment of Cookies Made from Composite Flours Containing Malted Barley Flour and Wheat Flour
Authors: Jukić Marko , Nakov Gjore , Komlenić Daliborka Koceva , Vasileva Nastia , Šumanovac Franjo , Lukinac Jasmina
Published: March 2022

Wheat-based short-dough cookies are considered low nutritional value foods because their recipes are high in fat and sugar. The aim of this study was to investigate the effects of replacing part of the wheat flour (WF) with different types of malted barley flour (MBF), while reducing sucrose in the recipe, in order to produce cookies with increased nutritional value, enhanced functional properties, and acceptable technological and sensory characteristics. Three types of brewer’s MBF (Pilsen, Amber, and Black) were used to replace WF in amounts of 20, 40, and 60%, while simultaneously reducing the addition of sucrose. Sucrose was added at levels of 66.6, 33.3, and 0% of the original standard recipe. MBF mitigated the effects of the reduced sucrose addition, likely due to its own high sugar content derived from barley malt. Snapping force determined with a texture analyzer decreased proportionally to sucrose reduction and MBF addition, indicating a softer texture of the cookies. MBF significantly increased the total phenolic content (TPC) and antioxidant activity (AOA) of the cookies. The results of the sensory analysis showed that cookies with Pilsen MBF and Amber MBF had a pleasantly sweet and rich flavor, while the addition of Black MBF produced an exaggerated bitter flavor and a nutty roasted aroma. The results suggest that different types of brewer’s MBF can be successfully used to produce functional cookies with reduced sucrose addition.
Sequence Capture of Mitochondrial Genome with PCR-Generated Baits Provides New Insights into the Biogeography of the Genus Abies Mill.
Authors: Semerikov Vladimir L. , Semerikova Svetlana A. , Khrunyk Yuliya Y. , Putintseva Yuliya A.
Published: March 2022

Mitochondrial DNA (mtDNA), being maternally inherited in plants of the family Pinaceae, is an important source of phylogeographic information. However, its use is hindered by a low mutation rate and frequent structure rearrangements. In the present study, we tested the method of genomic libraries enrichment with mtDNA via the sequence capture method yielding mtDNA data which were further used to reconstruct the phylogenetic tree of the genus Abies. The baits for hybrid capture were obtained by long-range PCR using primers designed on the basis of the assembly of Abies sibirica Ledeb. mitochondrial genome. Mitochondrial genomes of Picea sitchensis (Bong.) Carr., Larix sibirica Ledeb., and Keteleeria davidiana (Bertrand) Beissn. were used as an outgroup. The resulting phylogenetic tree consists of two sister branches, including the Eurasian and American species, respectively, with some exceptions. The subclade of A. sachalinensis (F. Schmidt) Mast. and A. veitchii Lindl. (Japan and Sakhalin islands) occupies a basal position in the branch of American firs, probably due to the complex history of fir migrations from North America to Eurasia. The tree has high support for majority of clades. For species represented by more than one sample an intraspecific variability was found which is suitable to design mtDNA markers for phylogeographic and population studies.
Development and Investigation of Zein and Cellulose Acetate Polymer Blends Incorporated with Garlic Essential Oil and β-Cyclodextrin for Potential Food Packaging Application
Authors: Marques Clara Suprani , Silva Rafael Resende Assis , Arruda Tarsila Rodrigues , Ferreira Ana Luiza Valadares , Oliveira Taíla Veloso de , Moraes Allan Robledo Fialho , Dias Marali Vilela , Vanetti Maria Cristina Dantas , Soares Nilda de Fátima Ferreira
Published: March 2022

The obtainment of new materials with distinct properties by mixing two or more polymers is a potential strategy in sustainable packaging research. In the present work, a blend of cellulose acetate (CA) and zein (60:40 wt/wt CA:zein) was manufactured by adding glycerol or tributyrin as plasticizers (30% wt/wt), and garlic essential oil (GEO), complexed (IC) or not with β-cyclodextrin (βCD), to produce active packaging. Blends plasticized with tributyrin exhibited a more homogeneous surface than those containing glycerol, which showed major defects. The blends underperformed compared with the CA films regarding mechanical properties and water vapor permeability. The presence of IC also impaired the films’ performance. However, the blends were more flexible than zein brittle films. The films added with GEO presented in vitro activity against Listeria innocua and Staphylococcus aureus. The IC addition into films, however, did not ensure antibacterial action, albeit that IC, when tested alone, showed activity against both bacteria. These findings suggest that the mixture of CA and plasticizers could increase the range of application of zein as a sustainable packaging component, while essential oils act as a natural bioactive to produce active packaging.