bluuebunny/crossref_metadata_2025 huggingface dataset:
Advantages and Limitations of Current Microgravity Platforms for Space Biology Research
Authors: Ferranti Francesca , Del Bianco Marta , Pacelli Claudia
Published: December 2020

Human Space exploration has created new challenges and new opportunities for science. Reaching beyond the Earth’s surface has raised the issue of the importance of gravity for the development and the physiology of biological systems, while giving scientists the tools to study the mechanisms of response and adaptation to the microgravity environment. As life has evolved under the constant influence of gravity, gravity affects biological systems at a very fundamental level. Owing to limited access to spaceflight platforms, scientists rely heavily on on-ground facilities that reproduce, to a different extent, microgravity or its effects. However, the technical constraints of counterbalancing the gravitational force on Earth add complexity to data interpretation. In-flight experiments are also not without their challenges, including additional stressors, such as cosmic radiation and lack of convection. It is thus extremely important in Space biology to design experiments in a way that maximizes the scientific return and takes into consideration all the variables of the chosen setup, both on-ground or on orbit. This review provides a critical analysis of current ground-based and spaceflight facilities. In particular, the focus was given to experimental design to offer the reader the tools to select the appropriate setup and to appropriately interpret the results.
Can Pre-Storm Errors in the Low-Level Inflow Help Predict Spatial Displacement Errors in MCS Initiation?
Authors: Vertz Nicholas J. , Gallus William A. , Squitieri Brian J.
Published: December 2020

The Great Plains low-level jet (LLJ) is a contributing factor to the initiation and evolution of nocturnal Mesoscale Convective Systems (MCSs) in the central United States by supplying moisture, warm air advection, and a source of convergence. Thus, the ability of models to correctly depict thermodynamics in the LLJ likely influences how accurately they forecast MCSs. In this study, the Weather Research and Forecasting (WRF) model was used to examine the relationship between spatial displacement errors for initiating simulated MCSs, and errors in forecast thermodynamic variables up to three hours before downstream MCS initiation in 18 cases. Rapid Update Cycle (RUC) analyses in 3 layers below 1500 m above ground level were used to represent observations. Correlations between simulated MCS initiation spatial displacements and errors in the magnitude of forecast thermodynamic variables were examined in regions near and upstream of both observed and simulated MCSs, and were found to vary depending on the synoptic environment. In strongly-forced cases, large negative moisture errors resulted in simulated MCSs initiating further downstream with respect to the low-level flow from those observed. For weakly-forced cases, correlations were weaker, with a tendency for smaller negative moisture errors to be associated with larger displacement errors to the right of the inflow direction for initiating MCSs.
Evaluation of Multiple Approaches to Estimate Daily Solar Radiation for Input to Crop Process Models
Authors: Perdinan  , Winkler Julie A. , Andresen Jeffrey A.
Published: December 2020

Daily solar radiation is a critical input for estimating plant growth and development, yet this variable is infrequently measured compared to other climate variables. This study evaluates the sensitivity of simulated maize and soybean production from the CERES-Maize and CROPGRO-Soybean modules of the Decision Support System for Agrotechnology Transfer (DSSAT) to daily solar radiation estimates obtained from traditional (stochastic, empirical, and mechanistic models) and non-traditional (satellite estimation, reanalysis datasets, and regional climate model simulations) approaches, using as an example radiation estimates for Hancock, Wisconsin, USA. When compared to observations, radiation estimates obtained from empirical and mechanistic models and a satellite-based dataset generally had smaller biases than other approaches. Daily solar radiation estimates from a reanalysis dataset and regional climate model simulations overestimate incoming daily solar radiation. When the radiation estimates were used as an input to CERES-Maize, no significant differences were found for maize yield obtained from the different radiation estimates compared to yield from observed radiation, even though differences were found in the daily values of leaf area index, crop evapotranspiration, and crop dry weight (biomass). In contrast, significant differences were found in simulated soybean yield from CROPGRO-Soybean for the majority of the radiation estimates.
Electrochemical Approaches for Rapid Characterization of Oxidizable Compounds in Different Oak Alternatives
Authors: Wirth Jéremie , Slaghenaufi Davide , Vidal Stéphane , Ugliano Maurizio
Published: December 2020

Oak alternatives (OAs) such as chips, granulates or staves, are becoming increasingly used in the wine industry. Although they are mostly considered for their contribution to wine aroma, they are also a source of phenolic compounds to be released in the wine, in particular ellagitannins contributing to wine mouthfeel and antioxidant characteristics. In the present study, we explore the potential for a rapid analytical method based on linear sweep voltammetry (LSV) combined with disposable sensors to provide a rapid measure of the oxidizable compounds present in different OAs, as well as their characterization. Strong correlations were found between the tannin content of different OAs and the total current measures during LSV analysis, allowing a rapid quantification of the oxidizable compounds present, mostly ellagitannin. Application of derivatization to raw voltammograms allowed extraction of a number of features that can be used for classification purposes, in particular with respect to OAs types (chips or staves) and degree of toasting.
Priorities in the Interdisciplinary Approach of Specific Learning Disorders (SLD) in Children with Type I Diabetes Mellitus (T1DM). From Theory to Practice
Authors: Tatsiopoulou Paraskevi , Porfyri Georgia-Nektaria , Bonti Eleni , Diakogiannis Ioannis
Published: December 2020

Background: A considerable endeavor had taken place in order to understand the associated challenges for children and adolescents with Specific Learning Disorder (SLD) and Type 1 Diabetes Mellitus (T1DM) but also in order to describe the necessary skills and approaches that the care givers have to develop to assist both children and parents. (1) Aim: The aim of this review is twofold. Firstly, to highlight the T1DM’s potential impact on psychological well-being, on cognitive functioning and on school performance in children and adolescents who confront SLD. Secondly, to discuss the necessity of a multidiscipline approach of poor school performance in students with SLD and T1DM, presenting the serious contribution of care providers: (a) parents/carers in the family setting, (b) teachers and psychologists in the school setting and (c) health specialists (pediatricians, nutricians, nurses, child psychiatrists and psychologists) in the medical setting. (2) Methods: In this narrative literature review of 12 selected articles, each one studies a special aspect of approach, during the diagnosis and the treatment of individuals with T1DM and SLD. The review concerns the arising problems and difficulties in the adherence to diagnosis, the management of insulin, the mental and physical wellbeing, the school performance, the cognitive functioning and learning difficulties of patients. We tried to synthesize an interdisciplinary approach that involves collaboration between family, school and medical frame; facilitating children’s and adolescents’ difficulties management, as well as parent and teacher involvement during the intervention implementation. (3) Results: The main issues of concern were examined through the available literature, as different factors had to be re-examined in the previous studies, regarding the potential impact of T1DM in cognitive and psychological functioning, as well as the effects of the intervention/approach/treatment of children and adolescents with SLD and T1DM. (4) Conclusions: Although T1DM diagnosis and demanding treatment are a heavy burden for children and their families, T1DM may or may not be associated with a variety of academic and psychological outcomes. Despite the variability of the reviewed research design quality, it was clearly defined that the impact of T1DM is not uniform across educational and mental variables. Strengthening the children’s physical, psychological and social wellbeing is an especially important factor, as it facilitates the insulin’s management as well as the learning difficulties. This is possible by supporting the parental and teacher involvement in the intervention process. This review highlights the need to reduce the distance between theory/research and practice, in some of the proposed areas in this field of knowledge.
Effects of Surgery on the 30-Day Survival Rate in Spontaneous Supratentorial Intracerebral Hemorrhage
Authors: Balasa Adrian , Ghiga Dana , Andone Razvan-Sebastian , Zahan Ancuta , Florian Ioan , Chinezu Rares
Published: December 2020

Background: Spontaneous intracerebral hemorrhage (ICH) is a severe form of stroke. The efficacy of surgery as ICH treatment is controversial. We sought to compare the 30-day postoperative mortality rate between patients with surgically and medically treated ICH; Methods: This prospective study enrolled patients consecutively diagnosed with ICH and treated between 2017 and 2019. Patients meeting the study surgical indications were assigned to either surgical or medical treatment. The relationship between Glasgow Coma Scale (GCS) score, age, ICH location, ICH volume, and 30-day mortality was analyzed. Results: A total of 174 ICH patients were enrolled in this study. Of these, 136 met the surgery criteria; 65 of these underwent surgery (Group A), and 71 received medical treatment (Group B). Age and ICH location did not modify mortality. Although surgery did not overall improve mortality some better postsurgical outcomes were observed among patients surgically treated with GCS scores of at least 10 points and ICH volumes between 30 to 50 mL; Conclusions: Despite achieving an immediate reduction in intracranial pressure, surgery seems to be advantageous only for patients with ICH volumes between 30 to 50 mL and GCS scores of 10 points or higher;
Aerobic Exercise Prevents Depression via Alleviating Hippocampus Injury in Chronic Stressed Depression Rats
Authors: Kang Jie , Wang Di , Duan Yongchang , Zhai Lin , Shi Lin , Guo Fei
Published: December 2020

(1) Background: Depression is one of the overwhelming public health problems. Alleviating hippocampus injury may prevent depression development. Herein, we established the chronic unpredictable mild stress (CUMS) model and aimed to investigate whether aerobic exercise (AE) could alleviate CUMS induced depression-like behaviors and hippocampus injury. (2) Methods: Forty-eight healthy male Sprague-Dawley rats (200 ± 20 g) were randomly divided into 4 groups (control, CUMS, CUMS + 7 days AE, CUMS + 14 days AE). Rats with AE treatments were subjected to 45 min treadmill per day. (3) Results: AE intervention significantly improved CUMS-induced depressive behaviors, e.g., running square numbers and immobility time assessed by the open field and forced swimming test, suppressed hippocampal neuron apoptosis, reduced levels of phosphorylation of NMDA receptor and homocysteine in hippocampus, as well as serum glucocorticoids, compared to the CUMS rats. In contrast, AE upregulated phosphorylation of AMPAR receptor and brain-derived neurotrophic factor (BDNF) hippocampus in CUMS depression rats. The 14 day-AE treatment exhibited better performance than 7 day-AE on the improvement of the hippocampal function. (4) Conclusion: AE might be an efficient strategy for prevention of CUMS-induced depression via ameliorating hippocampus functions. Underlying mechanisms may be related with glutamatergic system, the neurotoxic effects of homocysteine, and/or influences in glucocorticoids-BDNF expression interaction.
A Competency Model for the Selection and Performance Improvement of Project Managers in Collaborative Construction Projects: Behavioral Studies in Norway and Finland
Authors: Moradi Sina , Kähkönen Kalle , Klakegg Ole , Aaltonen Kirsi
Published: December 2020

Collaborative work practices are getting more common in construction projects. Consequently, new project delivery models have emerged and new practices have also entered the world of traditional delivery models. The resultant collaborative construction projects provide a different working environment compared to the traditional construction delivery models. This different environment seems to require project managers with certain types of competencies, but there is currently very limited research-based knowledge concerning this subject. This study aims at identifying such competencies, which project managers of collaborative construction projects need to possess to succeed. For this purpose, a human behavioral approach was employed where project managers’ behavior in their everyday work was the main source for understanding their competencies. Accordingly, the survey strategy was utilized, where a self-evaluation questionnaire was sent to 33 project managers of collaborative construction projects, and a response rate of 73% was achieved. The findings present four groups of competencies within a matrix model, structured based on their contribution to project managers’ successful performance in collaborative construction projects and the degree to which they can develop those competencies. The developed model can provide a baseline for selecting project managers and for enhancing the performance of the current ones.
Impact of Low-Dose Irradiation of the Lung and Heart on Toxicity and Pulmonary Function Parameters after Thoracic Radiotherapy
Authors: Schröder Christina , Buchali André , Windisch Paul , Vu Erwin , Basler Lucas , Zwahlen Daniel R. , Förster Robert
Published: December 2020

Objective: To assess the impact of (low) dose irradiation to the lungs and heart on the incidence of pneumonitis and pulmonary function changes after thoracic radiotherapy (RT). Methods/Material: Data of 62 patients treated with curative thoracic radiotherapy were analyzed. Toxicity data and pulmonary function tests (PFTs) were obtained before RT and at 6 weeks, at 12 weeks, and at 6 months after RT. PFTs included ventilation (e.g., vital capacity) and diffusion parameters (e.g., diffusion capacity for carbon monoxide (DLCO)). Dosimetric data of the lung and heart were extracted to assess the impact of dose on PFT changes and radiation pneumonitis (RP). Results: No statistically significant correlations between dose parameters and changes in ventilation parameters were found. There were statistically significant correlations between DLCO and low-dose parameters of the lungs (V5Gy–V30Gy (%)) and irradiation of the heart during the follow-up up to 6 months after RT, as well as a temporary correlation of the V60Gy (%) on the blood gas parameters at 12 weeks after RT. On multivariate analysis, both heart and lung parameters had a significant impact on DLCO. There was no statistically significant influence of any patient or treatment-related (including dose parameters) factors on the incidence of ≥G2 pneumonitis. Conclusion: There seems to be a lasting impact of low dose irradiation to the lung as well as irradiation to the heart on the DLCO after thoracic radiotherapy. No influence on RP was found in this analysis.
Cyclic Hypoxia: An Update on Its Characteristics, Methods to Measure It and Biological Implications in Cancer
Authors: Bader Samuel B. , Dewhirst Mark W. , Hammond Ester M.
Published: December 2020

Regions of hypoxia occur in most if not all solid cancers. Although the presence of tumor hypoxia is a common occurrence, the levels of hypoxia and proportion of the tumor that are hypoxic vary significantly. Importantly, even within tumors, oxygen levels fluctuate due to changes in red blood cell flux, vascular remodeling and thermoregulation. Together, this leads to cyclic or intermittent hypoxia. Tumor hypoxia predicts for poor patient outcome, in part due to increased resistance to all standard therapies. However, it is less clear how cyclic hypoxia impacts therapy response. Here, we discuss the causes of cyclic hypoxia and, importantly, which imaging modalities are best suited to detecting cyclic vs. chronic hypoxia. In addition, we provide a comparison of the biological response to chronic and cyclic hypoxia, including how the levels of reactive oxygen species and HIF-1 are likely impacted. Together, we highlight the importance of remembering that tumor hypoxia is not a static condition and that the fluctuations in oxygen levels have significant biological consequences.
The Association between Ranitidine Use and Gastrointestinal Cancers
Authors: McGwin Gerald
Published: December 2020

N-nitrosodimethylamine (NDMA) is a carcinogen in experimental animals. It has been classified a probable human carcinogen and has been found in ranitidine. This study sought to evaluate the association between ranitidine use and cancer of the gastrointestinal system. Events reported to the FDA Adverse Events Reporting System that were associated with the use of proton pump inhibitors (PPIs) and H2 antagonists were selected. Proportionate reporting ratios (PRRs) and associated 95% confidence intervals (CIs) were calculated to compare the proportion of all reported adverse events that were for gastrointestinal system cancers among adverse event reports for ranitidine to adverse event reports for other H2 antagonists. The proportion of adverse events for any gastrointestinal system cancer relative to all other events was elevated for ranitidine compared to PPIs and other H2 antagonists (PRR 3.66, 95% CI 3.19–4.20). Elevated and significant PRRs were observed for pharyngeal (PRR 9.24), esophageal (PRR 3.56), stomach (PRR 1.48), colorectal (PRR 16.31), liver (PRR 2.64), and pancreatic (PRR 2.18) cancers. The PRRs for anal (PRR 4.62) and gallbladder (PRR 4.62) cancer were also elevated though not statistically significant. In conjunction with a large body of epidemiologic and human and animal basic science research, the study results support the hypothesis that NDMA-contaminated ranitidine increases the risk of cancer and supports the withdrawal of these medications from the market.
High Risk α-HPV E6 Impairs Translesion Synthesis by Blocking POLη Induction
Authors: Wendel Sebastian O. , Snow Jazmine A. , Bastian Tyler , Brown Laura , Hernandez Candy , Burghardt Emily , Kahn Andrew , Murthy Vaibhav , Neill Daniel , Smith Zachary C. , Ault Kevin , Tawfik Ossama , Wu Cen , Wallace Nicholas A.
Published: December 2020

High risk genus α human papillomaviruses (α-HPVs) express two versatile oncogenes (α-HPV E6 and E7) that cause cervical cancer (CaCx) by degrading tumor suppressor proteins (p53 and RB). α-HPV E7 also promotes replication stress and alters DNA damage responses (DDR). The translesion synthesis pathway (TLS) mitigates DNA damage by preventing replication stress from causing replication fork collapse. Computational analysis of gene expression in CaCx transcriptomic datasets identified a frequent increased expression of TLS genes. However, the essential TLS polymerases did not follow this pattern. These data were confirmed with in vitro and ex vivo systems. Further interrogation of TLS, using POLη as a representative TLS polymerase, demonstrated that α-HPV16 E6 blocks TLS polymerase induction by degrading p53. This doomed the pathway, leading to increased replication fork collapse and sensitivity to treatments that cause replication stress (e.g., UV and Cisplatin). This sensitivity could be overcome by the addition of exogenous POLη.
PD-L1 Multiplex and Quantitative Image Analysis for Molecular Diagnostics
Authors: Abdullahi Sidi Fatima , Bingham Victoria , Craig Stephanie G. , McQuaid Stephen , James Jacqueline , Humphries Matthew P. , Salto-Tellez Manuel
Published: December 2020

Multiplex immunofluorescence (mIF) and digital image analysis (DIA) have transformed the ability to analyse multiple biomarkers. We aimed to validate a clinical workflow for quantifying PD-L1 in non-small cell lung cancer (NSCLC). NSCLC samples were stained with a validated mIF panel. Immunohistochemistry (IHC) was conducted and mIF slides were scanned on an Akoya Vectra Polaris. Scans underwent DIA using QuPath. Single channel immunofluorescence was concordant with single-plex IHC. DIA facilitated quantification of cell types expressing single or multiple phenotypic markers. Considerations for analysis included classifier accuracy, macrophage infiltration, spurious staining, threshold sensitivity by DIA, sensitivity of cell identification in the mIF. Alternative sequential detection of biomarkers by DIA potentially impacted final score. Strong concordance was observed between 3,3’-Diaminobenzidine (DAB) IHC slides and mIF slides (R2 = 0.7323). Comparatively, DIA on DAB IHC was seen to overestimate the PD-L1 score more frequently than on mIF slides. Overall, concordance between DIA on DAB IHC slides and mIF slides was 95%. DIA of mIF slides is rapid, highly comparable to DIA on DAB IHC slides, and enables comprehensive extraction of phenotypic data and specific microenvironmental detail intrinsic to the sample. Exploration of the clinical relevance of mIF in the context of immunotherapy treated cases is warranted.
Glioblastoma CUSA Fluid Protein Profiling: A Comparative Investigation of the Core and Peripheral Tumor Zones
Authors: La Rocca Giuseppe , Simboli Giorgia Antonia , Vincenzoni Federica , Rossetti Diana Valeria , Urbani Andrea , Ius Tamara , Della Pepa Giuseppe Maria , Olivi Alessandro , Sabatino Giovanni , Desiderio Claudia
Published: December 2020

The present investigation aimed to characterize the protein profile of cavitating ultrasound aspirator fluid of newly diagnosed and recurrent glioblastoma comparing diverse zones of collection, i.e., tumor core and tumor periphery, with the aid of 5-aminolevulinic acid fluorescence. The samples were pooled and analyzed in triplicate by LC-MS following the shotgun proteomic approach. The identified proteins were then grouped to disclose elements exclusive and common to the tumor state or tumor zones and submitted to gene ontology classification and pathway overrepresentation analysis. The proteins common to the distinct zones were further investigated by relative quantitation, following a label free approach, to disclose possible differences of expression. Nine proteins, i.e., tubulin 2B chain, CD59, far upstream element-binding, CD44, histone H1.4, caldesmon, osteopontin, tropomyosin chain and metallothionein-2, marked the core of newly diagnosed glioblastoma with respect to tumor periphery. Considering the tumor zone, including the core and the fluorescence positive periphery, the serine glycine biosynthesis, pentose phosphate, 5-hydroxytryptamine degredation, de novo purine biosynthesis and huntington disease pathways resulted statistically significantly overrepresented with respect to the human genome of reference. The fluorescence negative zone shared several protein elements with the tumor zone, possibly indicating the presence of pathological aspects of glioblastoma rather than of normal brain parenchyma. On the other hand, its exclusive protein elements were considered to represent the healthy zone and, accordingly, exhibiting no pathways overrepresentation. On the contrary to newly diagnosed glioblastoma, pathway overrepresentation was recognized only in the healthy zone of recurrent glioblastoma. The TGFβ signaling pathway, exclusively classified in the fluorescence negative periphery in newly diagnosed glioblastoma, was instead the exclusive pathway classified in the tumor core of recurrent glioblastoma. These results, preliminary obtained on sample pools, demonstrated the potential of cavitron ultrasonic surgical aspirate fluid for proteomic profiling of glioblastoma able to distinguish molecular features specific of the diverse tumor zones and tumor states, possibly contributing to the understanding of the highly infiltrative capability and recurrent rate of this aggressive brain tumor and opening to potential clinical applications to be further investigated.
Molecular Remission Using Low-Dose Immunotherapy with Minimal Toxicities for Poor Prognosis IGHV—Unmutated Chronic Lymphocytic Leukemia
Authors: Maharaj Dipnarine , Srinivasan Gayathri , Abreu Maria M. , Ko Meng-Wei , Jewett Anahid , Gouvea Jacqueline
Published: December 2020

Chronic lymphocytic leukemia (CLL) accounts for 10% of hematologic malignancies. CLL is a malignancy of CD5+ B cells and it is characterized by the accumulation of small, mature-appearing neoplastic lymphocytes in the blood, bone marrow, and secondary lymphoid tissues. In the present case, a middle-aged female patient with poor prognosis unmutated IGHV CLL achieved cytogenetic and molecular remission with minimal adverse events following six cycles of low dose recombinant human IL-2 (rIL-2) in combination with low dose targeted venetoclax. Personalized low dose rIL-2 in combination with either lenalidomide or venetoclax mediates natural killer stimulation and is an effective non-toxic immunotherapy administered in the outpatient setting for poor prognosis CLL.
TIME Is a Great Healer—Targeting Myeloid Cells in the Tumor Immune Microenvironment to Improve Triple-Negative Breast Cancer Outcomes
Authors: Singh Swarnima , Zhang Xiang H. F. , Rosen Jeffrey M.
Published: December 2020

The word myeloid is derived from the Greek word muelós which means “marrow”. Therefore, myeloid cells are described as cells that arise in the bone marrow. They can be distinguished from lymphoid cells based on their different differentiation trajectories—Lymphoid cells (B and T cells) are usually born in the bone marrow, but they need to migrate to lymphoid organs to mature and differentiate usually in response to antigens produced due to infections and diseases like cancer. On the other hand, myeloid cells do not follow this differentiation trajectory. They arise from the bone marrow, and do not need an encounter with antigens to gain their functionality. Thus, while lymphoid cells are a part of the adaptive immune system, myeloid cells are a part of the innate immune system. Hematopoiesis gives rise to two progenitor cells—the common myeloid progenitor (CMP) and the common lymphoid progenitor (CLP). The CMP can give rise to megakaryocytes, erythrocytes, mast cells and myeloblasts. Myeloblasts in turn lead to the formation of basophils, neutrophils, eosinophils and monocytes that can further differentiate into macrophages. This review will focus on macrophages as well as the phenotypes they acquire with the tumor immune microenvironment (TIME) in triple-negative breast cancer (TNBC). It will address how cancer cells in the tumor microenvironment (TME) recruit macrophages and may switch to recruiting neutrophils upon depletion of these tumor-associated macrophages (TAMs). Finally, it will also shed light on past and current treatment options that specifically target these cells and how those affect patient outcomes in TNBC.
Effect of Voltage Pulse Width and Synchronized Substrate Bias in High-Power Impulse Magnetron Sputtering of Zirconium Films
Authors: Kuo Chin-Chiuan , Lin Chun-Hui , Chang Jing-Tang , Lin Yu-Tse
Published: December 2020

The Zr film microstructure is highly influenced by the energy of the plasma species during the deposition process. The influences of the discharge pulse width, which is the key factor affecting ionization of sputtered species in the high-power impulse magnetron sputtering (HiPIMS) process, on the obtained microstructure of films is investigated in this research. The films deposited at different argon pressure and substrate biasing are compared. With keeping the same average HiPIMS power and duty cycle, the film growth rate of the Zr film decreases with increasing argon pressure and enhancing substrate biasing. In addition, the film growth rate decreases with the elongating HiPIMS pulse width. For the deposition at 1.2 Pa argon, extending the pulse width not only intensifies the ion flux toward the substrate but also increases the fraction of highly charged ions, which alter the microstructure of films from individual hexagonal prism columns into a tightly connected irregular column. Increasing film density leads to higher hardness. Sufficient synchronized negative substrate biasing and longer pulse width, which supports higher mobility of adatoms, causes the preferred orientation of hexagonal α-phase Zr films from (0 0 0 2) to (1 0 1¯ 1). Unlike the deposition at 1.2 Pa, highly charged ions are also found during the short HiPIMS pulse width at 0.8 Pa argon.
Impact of Surface Roughness on Crystal Nucleation
Authors: Grosfils Patrick , Lutsko James F.
Published: December 2020

We examine the effect of rough surfaces on crystal nucleation by means of kinetic Monte Carlo simulations. Our work makes use of three-dimensional kMC models, explicit representation of transport in solution and rough surfaces modeled as randomly varying height fluctuations (roughness) with exponentially decaying correlation length (topology). We use Forward-Flux Sampling to determine the nucleation rate for crystallization for surfaces of different roughness and topology and show that the effect on crystallization is a complex interplay between the two. For surfaces with low roughness, small clusters form on the surface but as clusters become larger they are increasingly likely to be found in the bulk solution while rougher surfaces eventually favor heterogeneous nucleation on the surface. In both cases, the rough surface raises the local supersaturation in the solution thus leading to another mechanism of enhanced nucleation rate.
Effects of Dietary L-Carnitine Supplementation on Platelets and Erythrogram of Dairy Cows with Special Emphasis on Parturition
Authors: Kononov Susanne Ursula , Meyer Jennifer , Frahm Jana , Kersten Susanne , Kluess Jeannette , Meyer Ulrich , Huber Korinna , Dänicke Sven
Published: December 2020

During late gestation and early lactation, many proliferative processes and metabolic adaptions are involved in homeorhesis. An adjusted supply of oxygen is a precondition for an optimized cellular energy metabolism whereby erythrocytes play a central role. Endogenous L-carnitine modulates the mitochondrial fatty acid utilization for generating adenosine triphosphate (ATP). As it might be insufficient around calving due to increased need, L-carnitine supplementation is frequently recommended. Thus, the present study addressed the interplay between the red hemogram, platelets, oxidative stress indices, and L-carnitine supplementation of dairy cows around calving. German Holstein cows were assigned to a control (n = 30) and an L-carnitine group (n = 29, 25 g of rumen-protected L-carnitine per cow and per day), and blood samples were taken from day 42 ante partum (ap) until day 110 postpartum (pp), with a higher sampling frequency during the first three days pp. The time courses of the erythrogram parameters reflected the physiological adaptations to the oxygen need without being influenced by L-carnitine supplementation. Erythrocytic antioxidative enzymatic defence paralleled the relative development of polycythemia ap, while non-enzymatic total plasma antioxidative capacity continuously increased pp. In contrast to erythrocytes, the platelet counts of the L-carnitine supplemented cows varied at significantly higher levels. This can be interpreted as a result of a membrane-stabilizing effect of L-carnitine.
Exergy Analysis of a Bio-System: Soil–Plant Interaction
Authors: Bararzadeh Ledari Masoomeh , Saboohi Yadollah , Valero Antonio , Azamian Sara
Published: December 2020

This paper explains a thorough exergy analysis of the most important reactions in soil–plant interactions. Soil, which is a prime mover of gases, metals, structural crystals, and electrolytes, constantly resembles an electric field of charge and discharge. The second law of thermodynamics reflects the deterioration of resources through the destruction of exergy. In this study, we developed a new method to assess the exergy of soil and plant formation processes. Depending on the types of soil, one may assess the efficiency and degradation of resources by incorporating or using biomass storage. According to the results of this study, during different processes from the mineralization process to nutrient uptake by the plant, about 62.5% of the input exergy will be destroyed because of the soil solution reactions. Most of the exergy destruction occurs in the biota–atmosphere subsystem, especially in the photosynthesis reaction, due to its low efficiency (about 15%). Humus and protonation reactions, with 14% and 13% exergy destruction, respectively, are the most exergy destroying reactions. Respiratory, weathering, and reverse weathering reactions account for the lowest percentage of exergy destruction and less than one percent of total exergy destruction in the soil system. The total exergy yield of the soil system is estimated at about 37.45%.
Information Theory for Human and Social Processes
Authors: Hilbert Martin
Published: December 2020

Ever since its earliest years, information theory has enjoyed both a promising and complicated relationship with the social sciences [...]
A Compact Component for Multi-Band Rejection and Frequency Coding in the Plasmonic Circuit at Microwave Frequencies
Authors: Tang Wenxuan , Hua Yujie , Cui Tie Jun
Published: December 2020

Plasmonic circuits, which support the propagation of spoof surface plasmon polaritons (SSPPs) at microwave frequencies, have been developed in recent years as an expected candidate for future highly integrated systems, mainly because of their extraordinary field confinements and sub-wavelength resolution. On the other hand, artificial electromagnetic (EM) resonators are widely adopted in metamaterial design for flexible resonance and band gaps. In this work, an electrically small complementary spiral, which is made up of six helix branches sculptured in the ground, is proposed to achieve independent resonances at six different frequency bands. Combined with the grounded corrugated transmission line (TL), the proposed component can provide designable multi-band rejection, and compose frequency coding circuits with a compact size (less than λ0/4). The complementary spirals excited with the bending TL and the straight one are both investigated, and independence band rejections and designed 6-bit coding sequences in the frequency spectrum are demonstrated numerically and experimentally. Hence, it is concluded that such compact components can be adopted to flexibly control the rejection of waves in multi-frequency bands, and benefits the development of frequency-identification circuits and systems.
Fast Design Closure of Compact Microwave Components by Means of Feature-Based Metamodels
Authors: Pietrenko-Dabrowska Anna , Koziel Slawomir
Published: December 2020

Precise tuning of geometry parameters is an important consideration in the design of modern microwave passive components. It is mandatory due to limitations of theoretical design methods unable to quantify certain phenomena that are important for the operation and performance of the devices (e.g., strong cross-coupling effects in miniaturized layouts). Consequently, the initial designs obtained using analytical or equivalent network models require further adjustment. For reliability reasons, it has to be conducted using electromagnetic (EM) simulation tools, which entails considerable computational expenses whenever conventional numerical optimization algorithms are employed. Accelerating EM-driven design procedures is therefore highly desirable. This work discusses a surrogate-based algorithm for fast design closure and dimension scaling of miniaturized microwave passives. Our approach involves a small database of previously obtained designs as well as two metamodels, an inverse one, employed to yield a high-quality initial design, and the forward surrogate that provides predictions of the system sensitivities. The second model is constructed at the level of response features, which enables a more accurate gradient estimation and leads to improved reliability and a faster convergence of the optimization process. The presented technique is validated using two compact microstrip couplers and benchmarked against the state-of-the-art warm-start optimization frameworks.
Multi-Scale Microfluidics for Transport in Shale Fabric
Authors: Ling Bowen , Khan Hasan J. , Druhan Jennifer L. , Battiato Ilenia
Published: December 2020

We develop a microfluidic experimental platform to study solute transport in multi-scale fracture networks with a disparity of spatial scales ranging between two and five orders of magnitude. Using the experimental scaling relationship observed in Marcellus shales between fracture aperture and frequency, the microfluidic design of the fracture network spans all length scales from the micron (1 μ) to the dm (10 dm). This intentional `tyranny of scales’ in the design, a determining feature of shale fabric, introduces unique complexities during microchip fabrication, microfluidic flow-through experiments, imaging, data acquisition and interpretation. Here, we establish best practices to achieve a reliable experimental protocol, critical for reproducible studies involving multi-scale physical micromodels spanning from the Darcy- to the pore-scale (dm to μm). With this protocol, two fracture networks are created: a macrofracture network with fracture apertures between 5 and 500 μm and a microfracture network with fracture apertures between 1 and 500 μm. The latter includes the addition of 1 μm ‘microfractures’, at a bearing of 55°, to the backbone of the former. Comparative analysis of the breakthrough curves measured at corresponding locations along primary, secondary and tertiary fractures in both models allows one to assess the scale and the conditions at which microfractures may impact passive transport.
A Comprehensive Health Indicator Integrated by the Dynamic Risk Profile from Condition Monitoring Data and the Function of Financial Losses
Authors: Liang Xiaoxia , Duan Fang , Bennett Ian , Mba David
Published: December 2020

Large rotating machinery, such as centrifugal gas compressors and pumps, have been widely applied and acted as crucial components in the oil and gas industries. Breakdowns or deteriorated performance of these rotating machines can bring significant economic loss to the companies. In order to conduct effective maintenance and avoid unplanned downtime, a system-wide health indicator is proposed in this paper. The health indicator not only uses a dynamic risk profile, but also considers financial loss and the fault probability based on condition monitoring data. This methodology is carried out by four steps: fault detection, probability of fault calculation, consequence of fault calculation and dynamic risk assessment. In our methodology, the fault probability is calculated by robust Mahalanobis distance, presenting as a system-wide feature from a sparse autoencoder fault detection model enabled early fault detection. The value of the health indicator is presented in financial loss, which assists in effective operational decision-making in a process system. To evaluate the performance of the proposed indicator, two case studies were carried out—one case tested on multivariate industrial data obtained from a pump, and another one tested on an industrial data set from a compressor. Results prove that the integrated health indicator can detect the faults at their incipient stages, indicate the degradation of the system with dynamically updated process risk at each sampling instant, and suggest an appropriate shutdown time before the system suffers severe damage. In addition, this methodology can be adapted to other machines’ health assessments, such as those of turbines and motors. The presented method of processing the industrial data set can benefit relevant readers.
Polyhydroxyalkanoated-Rich Microbial Cells from Bio-Based Volatile Fatty Acids as Potential Ingredient for Aquaculture Feed
Authors: Botturi Alice , Battista Federico , Andreolli Marco , Faccenda Filippo , Fusco Salvatore , Bolzonella David , Lampis Silvia , Frison Nicola
Published: December 2020

In this study, the production of polyhydroxyalkanoated PHA-rich microbial biomass as a novel feed additive in aquaculture was investigated at a lab-scale. Bio-based volatile fatty acids (VFAs), obtained from the acidogenic fermentation of agricultural residues in existing anaerobic digestion plants, were used as carbon and energy to cultivate the PHA-rich microbial biomass. The experimental activities were carried out using Thauera sp. Sel9 as pure strain, which was grown in a continuous stirred-tank reactor (CSTR) operated at three different hydraulic retention times (HRT). The highest productivity obtained of biomass cells was 0.69 g/L day, operating at one day HRT while the observed PHAs production yield was 0.14 gPHA/g soluble COD removed. At these conditions, the PHA concentration in the microbial cells was 41%. Although the sulfur amino acids were available at high concentrations and above the typical concentration found in fishmeal, the amino acids profile of the obtained biomass revealed a lack of histidine and threonine. A preliminary economic analysis showed that the production of a novel source of feed additive from the conversion of agro-residues could give higher benefits in terms of revenues compared to the production of biogas production through anaerobic digestion.
A New Method of Regulation of Loads Acting on the Shaft Lining in Sections Located in the Salt Rock Mass
Authors: Kamiński Paweł
Published: December 2020

Rock salt is characterized by specific geomechanical and rheological properties. Layers of rock salt at depths of over 900 m cause problems with shaft lining deformation. Methods of shaft lining protection used to date (e.g., in the Sieroszowice mine) have not been effective enough. The research presents a patented and copyright protected concept of a shaft lining construction that can be used in rock masses with strong rheological properties and susceptibility to leaching. A high value of convergence in salt rock mass is a reason for serious problems with shaft lining stability. Numerous trials have been done to provide appropriate shaft lining for salt layers, especially to ensure proper geometry of shaft members and conveyance guidance. In the new shaft lining concept, the excessive rock creep into the outbreak inside the shaft diameter is removed by local and controlled leaching of the shaft cheeks by means of fresh water through a porous medium at the contact layer behind the watertight tubing lining. The article presents the methodology of performing tests on a special device and the test results.
Discrimination and Determination of Extractive Content of Ebony (Diospyros celebica Bakh.) from Celebes Island by Near-Infrared Spectroscopy
Authors: Karlinasari Lina , Noviyanti Noviyanti , Purwanto Y. Aris , Majiidu Muhammad , Dwiyanti Fifi G. , Rafi Mohamad , Damayanti Ratih , Harnelly Essy , Siregar Iskandar Z.
Published: December 2020

Ebony (Diospyros celebica Bakh.) is an endemic plant on Celebes (Sulawesi) island. Extractive compounds within ebony wood cause it to have durability, strength, and beautiful patterns. In this study, we used near-infrared (NIR) spectroscopy to discriminate between ebony wood samples, based on their origins at different growth sites on Celebes island, and to develop quantitative models to predict the extractive content of ebony wood. A total of 45 wood meal samples from 11 sites located in West, Central, and South Celebes were collected in this study. NIR spectral data were acquired from hot water and ethanol–benzene soluble extracts from ebony wood in this study. The extractive content of the ebony was 10.408% and 10.774% based on hot water solubility and treatment with ethanol–benzene solvent, respectively. Multivariate analysis based on principal component analysis–discriminant analysis revealed that ebony wood from West Celebes differed from most of the wood from South Celebes; however, it was only slightly different from ebony wood from Central Celebes based on NIR spectra data. These findings were in line with the extractive contents obtained. Partial least square regression models based on wood meal spectra could potentially be used to estimate the hot water and ethanol–benzene extractive contents from ebony wood.
Research on the Malting Properties of Domestic Wheat Varities
Authors: Krstanović Vinko , Habschied Kristina , Dvojković Krešimir , Mastanjević Krešimir
Published: December 2020

This study aimed to establish the suitability of new Croatian wheat varieties for the production of pale wheat malt. Four new varieties/breeding lines from the Agricultural Institute Osijek were tested (Indira, OSK. 378/19, OSK. 346/20, and OSK. 353/20). The quality of starting wheat, the success of the micromalting process and the quality of finished malts were analyzed. The obtained results showed that the tested varieties/breeding lines meet the quality criteria for pale beer malt, and can be classified in the II malting quality group. The vast majority of quality indicators examined in this research showed values within the limits recommended for pale wheat malt. The obtained results were very good considering that these varieties are not intended for malting, nor in their selection process attention was paid to meet the brewing quality criteria. Due to the sensory characteristics of beer, the possibility of intervention during the malting process is limited, therefore when selecting wheat for malting, attention should be paid to the lowest possible content of total and soluble proteins, which can be achieved by appropriate agrotechnical measures in cultivation.
The Important Contribution of Non-Saccharomyces Yeasts to the Aroma Complexity of Wine: A Review
Authors: Borren Elliot , Tian Bin
Published: December 2020

Non-Saccharomyces yeast plays an important role in the initial stages of a wild ferment, as they are found in higher abundance in the vineyard than Saccharomyces cerevisiae. As such, there has been a focus in recent years to isolate these yeast species and characterize their effect on wine fermentation and subsequent aroma. This effect on wine aroma is often species and strain dependent, as the enzymatic profile of each yeast will determine which aroma compounds are formed as secondary metabolites. Semi-fermentative yeast, such as Hanseniaspora spp., Candida spp. and Metschnikowia pulcherrima, are commonly in high abundance in fresh grape must and have diverse enzymatic profiles, however they show a weak tolerance to ethanol, limiting their impact to the initial stages of fermentation. Fully fermentative non-Saccharomyces yeast, characterized by high ethanol tolerance, are often found at low abundance in fresh grape must, similar to Saccharomyces cerevisiae. Their ability to influence the aroma profile of wine remains high, however, due to their presence into the final stages of fermentation. Some fermentative yeasts also have unique oenological properties, such as Lanchancea thermotolerans and Schizosaccharomyces pombe, highlighting the potential of these yeast as inoculants for specific wine styles.