bluuebunny/crossref_metadata_2025 huggingface dataset:
Strategic Development of Aurantiochytrium sp. Mutants With Superior Oxidative Stress Tolerance and Glucose-6-Phosphate Dehydrogenase Activity for Enhanced DHA Production Through Plasma Mutagenesis Coupled With Chemical Screening
Authors: Nazir Yusuf , Phabakaran Pranesha , Halim Hafiy , Mohamed Hassan , Naz Tahira , Abdul Hamid Aidil , Song Yuanda
Published: April 2022

Thraustochytrids, such as Aurantiochytrium and Schizochytrium, have been shown as a promising sustainable alternative to fish oil due to its ability to accumulate a high level of docosahexaenoic acid (DHA) from its total fatty acids. However, the low DHA volumetric yield by most of the wild type (WT) strain of thraustochytrids which probably be caused by the low oxidative stress tolerance as well as a limited supply of key precursors for DHA biosynthesis has restricted its application for industrial application. Thus, to enhance the DHA production, we aimed to generate Aurantiochytrium SW1 mutant with high tolerance toward oxidative stress and high glucose-6 phosphate dehydrogenase (G6PDH) activities through strategic plasma mutagenesis coupled with chemical screening. The WT strain (Aurantiochytrium sp. SW1) was initially exposed to plasma radiation and was further challenged with zeocin and polydatin, generating a mutant (YHPM1) with a 30, 65, and 80% higher overall biomass, lipid, and DHA production in comparison with the parental strains, respectively. Further analysis showed that the superior growth, lipid, and DHA biosynthesis of the YHMP1 were attributed not only to the higher G6PDH and enzymes involved in the oxidative defense such as superoxide dismutase (SOD) and catalase (CAT) but also to other key metabolic enzymes involved in lipid biosynthesis. This study provides an effective approach in developing the Aurantiochytrium sp. mutant with superior DHA production capacity that has the potential for industrial applications.
Association Between Skeletal Muscle Mass and Severity of Steatosis and Fibrosis in Non-alcoholic Fatty Liver Disease
Authors: Guo Wen , Zhao Xin , Miao Mengyuan , Liang Xiuru , Li Xiaona , Qin Pei , Lu Jing , Zhu Wenfang , Wu Juan , Zhu Chen , Xu Nianzhen , Zhang Qun
Published: April 2022

Background

Sarcopenia is known to be the risk factor of non-alcoholic fatty liver disease (NAFLD). However, studies evaluating the association of skeletal muscle mass (SMM) with liver fibrosis by transient elastography are limited. Here, we investigated the association of SMM with hepatic steatosis and fibrosis assessed in Chinese adults.

Methods

Patients who underwent liver ultrasonography at the Health Promotion Center of the First Affiliated Hospital of Nanjing Medical University between January 2020 to June 2021 were enrolled. We used transient elastography to evaluate the degree of hepatic fat and liver stiffness. Appendicular skeletal muscle mass was determined by bioelectrical impedance and was adjusted for body weight to derive the skeletal muscle mass index (SMI).

Results

Of 3,602 finally enrolled individuals, 1,830 had NAFLD and 1,772 did not have NAFLD. SMI gradually decreased as the severity of hepatic steatosis increased (40.47 ± 3.94% vs. 39.89 ± 3.57% vs. 39.22 ± 3.46% vs. 37.81 ± 2.84%, P < 0.001). Individuals with F3-F4 and F2 liver fibrosis groups had significantly lower SMI than individuals with F0-F1 stages (37.51 ± 3.19% vs. 38.06 ± 3.51% vs. 39.36 ± 3.38%, P < 0.001). As the SMI increased, the percentages of subjects with mild and severe NAFLD, and the percentages of subjects in F2 and F3-F4 stage were gradually decreased. SMI was independently associated with the severity of hepatic steatosis and fibrosis by logistic regression analysis. Moreover, decreased SMI was an independent risk factor for NAFLD and fibrosis.

Conclusion

SMI is closely associated with liver fat content and liver fibrosis in Chinese adults with NAFLD.
Assessment of Solid Pulmonary Nodules or Masses Using Zero Echo Time MR Lung Imaging: A Prospective Head-to-Head Comparison With CT
Authors: Liu Qianyun , Feng Zhichao , Liu Weiyin Vivian , Fu Weidong , He Lei , Cheng Xiaosan , Mao Zhongliang , Zhou Wenming
Published: April 2022

Objective

The aim of this study is to determine the potential of zero echo time (ZTE) MR lung imaging in the assessment of solid pulmonary nodules or masses and diagnostic consistency to CT in terms of morphologic characterization.

Methods

Our Institutional Review Board approved this prospective study. Seventy-one patients with solid pulmonary nodules or masses larger than 1 cm in diameter confirmed by chest CT were enrolled and underwent further lung ZTE-MRI scans within 7 days. ZTE-MRI and CT images were compared in terms of image quality and imaging features. Unidimensional diameter and three-dimensional volume measurements on both modalities were manually measured and compared using the Wilcoxon signed-rank test, intraclass correlation coefficient (ICC), Pearson’s correlation analysis, and Bland–Altman analysis. Multivariable logistic regression analysis was used to identify the factors associated with significant inter-modality variation of volume.

Results

Fifty-four of 71 (76.1%) patients were diagnosed with lung cancer. Subjective image quality was superior in CT compared with ZTE-MRI (p < 0.001). Inter-modality agreement for the imaging features was moderate for emphysema (kappa = 0.50), substantial for fibrosis (kappa = 0.76), and almost perfect (kappa = 0.88-1.00) for the remaining features. The size measurements including diameter and volume between ZTE-MRI and CT showed no significant difference (p = 0.36 for diameter and 0.60 for volume) and revealed perfect inter-observer (ICC = 0.975–0.980) and inter-modality (ICC = 0.942–0.992) agreements. Multivariable analysis showed that non-smooth margin [odds ratio (OR) = 6.008, p = 0.015] was an independent predictor for the significant inter-modality variation of volume.

Conclusion

ZTE lung imaging is feasible as a part of chest MRI in the assessment and surveillance for solid pulmonary nodules or masses larger than 1 cm, presenting perfect agreement with CT in terms of morphologic characterization.
Real-Time Artificial Intelligence-Based Histologic Classifications of Colorectal Polyps Using Narrow-Band Imaging
Authors: Lu Yi , Wu Jiachuan , Zhuo Xianhua , Hu Minhui , Chen Yongpeng , Luo Yuxuan , Feng Yue , Zhi Min , Li Chujun , Sun Jiachen
Published: April 2022

Background and Aims

With the development of artificial intelligence (AI), we have become capable of applying real-time computer-aided detection (CAD) in clinical practice. Our aim is to develop an AI-based CAD-N and optimize its diagnostic performance with narrow-band imaging (NBI) images.

Methods

We developed the CAD-N model with ResNeSt using NBI images for real-time assessment of the histopathology of colorectal polyps (type 1, hyperplastic or inflammatory polyps; type 2, adenomatous polyps, intramucosal cancer, or superficial submucosal invasive cancer; type 3, deep submucosal invasive cancer; and type 4, normal mucosa). We also collected 116 consecutive polyp videos to validate the accuracy of the CAD-N.

Results

A total of 10,573 images (7,032 images from 650 polyps and 3,541 normal mucous membrane images) from 478 patients were finally chosen for analysis. The sensitivity, specificity, PPV, NPV, and accuracy for each type of the CAD-N in the test set were 89.86%, 97.88%, 93.13%, 96.79%, and 95.93% for type 1; 93.91%, 95.49%, 91.80%, 96.69%, and 94.94% for type 2; 90.21%, 99.29%, 90.21%, 99.29%, and 98.68% for type 3; and 94.86%, 97.28%, 94.73%, 97.35%, and 96.45% for type 4, respectively. The overall accuracy was 93%. We also built models for polyps ≤5 mm, and the sensitivity, specificity, PPV, NPV, and accuracy for them were 96.81%, 94.08%, 95%, 95.97%, and 95.59%, respectively. Video validation results showed that the sensitivity, specificity, and accuracy of the CAD-N were 84.62%, 86.27%, and 85.34%, respectively.

Conclusions

We have developed real-time AI-based histologic classifications of colorectal polyps using NBI images with good accuracy, which may help in clinical management and documentation of optical histology results.
Metabolic Phenotype and Microbiome of Infants Fed Formula Containing Lactobacillus paracasei Strain F-19
Authors: Lee Hanna , Li Zailing , Christensen Britt , Peng Yongmei , Li Xiaonan , Hernell Olle , Lönnerdal Bo , Slupsky Carolyn M.
Published: April 2022

Early childhood nutrition drives the development of the gut microbiota. In contrast to breastfeeding, feeding infant formula has been shown to impact both the gut microbiota and the serum metabolome toward a more unfavorable state. It is thought that probiotics may alter the gut microbiota and hence create a more favorable metabolic outcome. To investigate the impact of supplementation with Lactobacillus paracasei spp. paracasei strain F-19 on the intestinal microbiota and the serum metabolome, infants were fed a formula containing L. paracasei F19 (F19) and compared to a cohort of infants fed the same standard formula without the probiotic (SF) and a breast-fed reference group (BF). The microbiome, as well as serum metabolome, were compared amongst groups. Consumption of L. paracasei F19 resulted in lower community diversity of the gut microbiome relative to the SF group that made it more similar to the BF group at the end of the intervention (4 months). It also significantly increased lactobacilli and tended to increase bifidobacteria, also making it more similar to the BF group. The dominant genus in the microbiome of all infants was Bifidobacterium throughout the intervention, which was maintained at 12 months. Although the serum metabolome of the F19 group was more similar to the group receiving the SF than the BF group, increases in serum TCA cycle intermediates and decreases in several amino acids in the metabolome of the F19 group were observed, which resulted in a metabolome that trended toward the BF group. Overall, L. paracasei F19 supplementation did not override the impact of formula-feeding but did impact the microbiome and the serum metabolome in a way that may mitigate some unfavorable metabolic impacts of formula-feeding.
Mechanisms Underlying Curcumin-Induced Neuroprotection in Cerebral Ischemia
Authors: Fan Feng , Lei Meng
Published: April 2022

Ischemic stroke is the leading cause of death and disability worldwide, and restoring the blood flow to ischemic brain tissues is currently the main therapeutic strategy. However, reperfusion after brain ischemia leads to excessive reactive oxygen species production, inflammatory cell recruitment, the release of inflammatory mediators, cell death, mitochondrial dysfunction, endoplasmic reticulum stress, and blood–brain barrier damage; these pathological mechanisms will further aggravate brain tissue injury, ultimately affecting the recovery of neurological functions. It has attracted the attention of researchers to develop drugs with multitarget intervention effects for individuals with cerebral ischemia. A large number of studies have established that curcumin plays a significant neuroprotective role in cerebral ischemia via various mechanisms, including antioxidation, anti-inflammation, anti-apoptosis, protection of the blood–brain barrier, and restoration of mitochondrial function and structure, restoring cerebral circulation, reducing infarct volume, improving brain edema, promoting blood–brain barrier repair, and improving the neurological functions. Therefore, summarizing the results from the latest literature and identifying the potential mechanisms of action of curcumin in cerebral ischemia will serve as a basis and guidance for the clinical applications of curcumin in the future.
Bioluminescent Zebrafish Transplantation Model for Drug Discovery
Authors: Hason Martina , Jovicic Jovana , Vonkova Ivana , Bojic Milan , Simon-Vermot Theresa , White Richard M. , Bartunek Petr
Published: April 2022

In the last decade, zebrafish have accompanied the mouse as a robust animal model for cancer research. The possibility of screening small-molecule inhibitors in a large number of zebrafish embryos makes this model particularly valuable. However, the dynamic visualization of fluorescently labeled tumor cells needs to be complemented by a more sensitive, easy, and rapid mode for evaluating tumor growth in vivo to enable high-throughput screening of clinically relevant drugs. In this study we proposed and validated a pre-clinical screening model for drug discovery by utilizing bioluminescence as our readout for the determination of transplanted cancer cell growth and inhibition in zebrafish embryos. For this purpose, we used NanoLuc luciferase, which ensured rapid cancer cell growth quantification in vivo with high sensitivity and low background when compared to conventional fluorescence measurements. This allowed us large-scale evaluation of in vivo drug responses of 180 kinase inhibitors in zebrafish. Our bioluminescent screening platform could facilitate identification of new small-molecules for targeted cancer therapy as well as for drug repurposing.
Nonlinear Anti-(Parity-Time) Symmetric Dimer
Authors: Rodrigues A. S. , Ross R. M. , Konotop V. V. , Saxena A. , Kevrekidis P. G.
Published: April 2022

In the present work we propose a nonlinear anti-PT-symmetric dimer, that at the linear level has been experimentally created in the realm of electric circuit resonators. We find four families of solutions, the so-called upper and lower branches, both in a symmetric and in an asymmetric (symmetry-broken) form. We unveil analytically and confirm numerically the critical thresholds for the existence of such branches and explore the bifurcations (such as saddle-node ones) that delimit their existence, as well as transcritical ones that lead to their potential exchange of stability. We find that out of the four relevant branches, only one, the upper symmetric branch, corresponds to a spectrally and dynamically robust solution. We subsequently leverage detailed direct numerical computations in order to explore the dynamics of the different states, corroborating our spectral analysis results.
Dissecting the Effects of Aldosterone and Hypokalemia on the Epithelial Na+ Channel and the NaCl Cotransporter
Authors: Kristensen Mathias , Fenton Robert A. , Poulsen Søren B.
Published: April 2022

Primary hyperaldosteronism (PA) is characterized by aldosterone excess and hypertension. This may be linked to increased renal Na+ reabsorption via the epithelial Na+ channel (ENaC) and the NaCl cotransporter (NCC). The majority of PA patients have normal plasma K+ levels, but a subset of cases are associated with hypokalemia. High NCC levels observed in long-term studies with aldosterone-infused rodents have been attributed to direct effects of aldosterone. Aldosterone can also increase active phosphorylated NCC (pT58-NCC) acutely. However, direct effects of aldosterone on NCC have been contested by recent studies indicating that it is rather an indirect effect of hypokalemia. We therefore set out to determine isolated long-term aldosterone and K+ effects on ENaC and NCC using various in vivo and ex vivo approaches. In mice, aldosterone-induced hypokalemia was prevented by simultaneous amiloride infusion, coupled to increased cleavage of α- and γENaC but no effect on NCC. Regression analyses of in vivo data showed a positive correlation between aldosterone/K+ and αENaC but a negative correlation with NCC and pT58-NCC. Ex vivo, exposure of kidney tubules for 21 h to aldosterone increased cleavage of αENaC and γENaC, but no effects were observed on NCC or pT58-NCC. Exposure of tubules to low K+ media reduced αENaC but increased NCC and pT58-NCC. As hypokalemia can enhance cell proliferation markers in the distal convoluted tubule (DCT), we hypothesized that aldosterone infusion would increase proliferating cell nuclear antigen (PCNA) expression. Infusion of aldosterone in mice for 6 days greatly increased PCNA expression in the DCT. Collectively, in vivo and ex vivo data suggest that both aldosterone and K+ can increase ENaC directly. In contrast, the observed increase in abundance and phosphorylation of NCC in aldosterone-infused mice is likely an indirect effect of enhanced ENaC-mediated K+ secretion and subsequent hypokalemia. Thus, it is possible that NCC may only be increased in PA when the condition is associated with hypokalemia.
Zfp580 Regulates Paracrine and Endocrine Igf1 and Igfbp3 Differently in the Brain and Blood After a Murine Stroke
Authors: Hoffmann Christian J. , Kuffner Melanie T. C. , Lips Janet , Lorenz Stephanie , Endres Matthias , Harms Christoph
Published: April 2022

Insulin-like growth factor 1 (Igf1) and insulin-like growth factor binding protein 3 (Igfbp3) are endocrine and paracrine factors that influence stroke occurrence, severity, and recovery. Low levels of endocrine Igf1 and Igfbp3 were associated with larger infarct volumes and unfavorable outcomes. Paracrine Igf1 is brain cytoprotective and improves functional recovery after stroke. In this study, we evaluated the effects of zinc finger protein 580 (Zfp580) on endocrine and paracrine Igf1 and Igfbp3 after stroke. Zfp580 suppressed the expression of Igf1 and Igfbp3 in cerebral microvascular endothelial cells (bEnd.3) as determined by real-time RT-PCR. Zfp580 was suppressed by combined oxygen and glucose deprivation (OGD) and mediated the effect of OGD on Igf1 and Igfbp3. In vivo, we evaluated paracrine regulation by real-time RT-PCR of brain lysates and endocrine regulation by ELISA of blood samples. Genomic ablation of Zfp580 did not alter basal paracrine or endocrine Igf1 and Igfbp3 levels. After transient middle cerebral artery occlusion (MCAo), Zfp580 was globally elevated in the brain for up to 3 days. Paracrine Igf1 and Igfbp3 were selectively induced in the ischemic hemisphere from day 2 to day 3 or day 1 to day 7, respectively. In Zfp580 knockout mice, the paracrine regulations of Igf1 and Igfbp3 were attenuated while endocrine Igf1 and the molar Igf1/Igfbp3 ratio were increased. In conclusion, Zfp580 differentially controls paracrine and endocrine Igf1 and Igfbp3 after stroke. Inhibition of Zfp580 might be a new treatment target leading to increased activity of Igf1 to improve stroke outcome.
Heterosis and Responses to Selection in Orange-Fleshed Sweetpotato (Ipomoea batatas L.) Improved Using Reciprocal Recurrent Selection
Authors: Grüneberg Wolfgang J. , De Boeck Bert , Diaz Federico , Eyzaguirre Raul , Low Jan W. , Reif Jochen C. , Campos Hugo
Published: April 2022

Sweetpotato is a highly heterozygous hybrid, and populations of orange-fleshed sweetpotato (OFSP) have a considerable importance for food security and health. The objectives were to estimate heterosis increments and response to selection in three OFSP hybrid populations (H1) developed in Peru for different product profiles after one reciprocal recurrent selection cycle, namely, H1 for wide adaptation and earliness (O-WAE), H1 for no sweetness after cooking (O-NSSP), and H1 for high iron (O-HIFE). The H1 populations were evaluated at two contrasting locations together with parents, foundation (parents in H0), and two widely adapted checks. Additionally, O-WAE was tested under two environmental conditions of 90-day and a normal 120-day harvest. In each H1, the yield and selected quality traits were recorded. The data were analyzed using linear mixed models. The storage root yield traits exhibited population average heterosis increments of up to 43.5%. The quality traits examined have exhibited no heterosis increments that are worth exploiting. The storage root yield genetic gain relative to the foundation was remarkable: 118.8% for H1-O-WAE for early harvest time, 81.5% for H1-O-WAE for normal harvest time, 132.4% for H1-O-NSSP, and 97.1% for H1-O-HIFE. Population hybrid breeding is a tool to achieve large genetic gains in sweetpotato yield via more efficient population improvement and allows a rapid dissemination of globally true seed that is generated from reproducible elite crosses, thus, avoiding costly and time-consuming virus cleaning of elite clones typically transferred as vegetative plantlets. The population hybrid breeding approach is probably applicable to other clonally propagated crops, where potential for true seed production exists.
CRISPR/Cas9-Mediated Editing of Autophagy Gene 6 in Petunia Decreases Flower Longevity, Seed Yield, and Phosphorus Remobilization by Accelerating Ethylene Production and Senescence-Related Gene Expression
Authors: Lin Yiyun , Jones Michelle L.
Published: April 2022

Developmental petal senescence is a type of programmed cell death (PCD), during which the production of ethylene is induced, the expression of PCD-related genes is upregulated, and nutrients are recycled. Autophagy is an intracellular mechanism involved in PCD modulation and nutrient cycling. As a central component of the autophagy pathway, Autophagy Gene 6 (ATG6) was previously shown as a negative regulator of petal senescence. To better understand the role of autophagy in ethylene biosynthesis and nutrient remobilization during petal senescence, we generated and characterized the knockout (KO) mutants of PhATG6 using CRISPR/Cas9 in Petunia × hybrida ‘Mitchell Diploid.’ PhATG6-KO lines exhibited decreased flower longevity when compared to the flowers of the wild-type or a non-mutated regenerative line (controls), confirming the negative regulatory role of ATG6 in petal senescence. Smaller capsules and fewer seeds per capsule were produced in the KO plants, indicating the crucial function of autophagy in seed production. Ethylene production and ethylene biosynthesis genes were upregulated earlier in the KO lines than the controls, indicating that autophagy affects flower longevity through ethylene. The transcript levels of petal PCD-related genes, including PhATG6, PhATG8d, PhPI3K (Phosphatidylinositol 3-Kinase), and a metacaspase gene PhMC1, were upregulated earlier in the corollas of PhATG6-KO lines, which supported the accelerated PCD in the KO plants. The remobilization of phosphorus was reduced in the KO lines, showing that nutrient recycling was compromised. Our study demonstrated the important role of autophagy in flower lifespan and seed production and supported the interactions between autophagy and various regulatory factors during developmental petal senescence.
Mitigation of Salinity-Induced Oxidative Damage, Growth, and Yield Reduction in Fine Rice by Sugarcane Press Mud Application
Authors: Khan Imran , Muhammad Awon , Chattha Muhammad Umer , Skalicky Milan , Bilal Chattha Muhammad , Ahsin Ayub Muhammad , Rizwan Anwar Muhammad , Soufan Walid , Hassan Muhammad Umair , Rahman Md Atikur , Brestic Marian , Zivcak Marek , El Sabagh Ayman
Published: April 2022

Salinity stress is one of the major global problems that negatively affect crop growth and productivity. Therefore, ecofriendly and sustainable strategies for mitigating salinity stress in agricultural production and global food security are highly demandable. Sugarcane press mud (PM) is an excellent source of the organic amendment, and the role of PM in mitigating salinity stress is not well understood. Therefore, this study was aimed to investigate how the PM mitigates salinity stress through the regulation of rice growth, yield, physiological properties, and antioxidant enzyme activities in fine rice grown under different salinity stress conditions. In this study, different levels of salinity (6 and 12 dS m–1) with or without different levels of 3, 6, and 9% of SPM, respectively were tested. Salinity stress significantly increased malondialdehyde (MDA, 38%), hydrogen peroxide (H2O2, 74.39%), Na+ (61.5%), electrolyte leakage (40.32%), decreased chlorophyll content (32.64%), leaf water content (107.77%), total soluble protein (TSP, 72.28%), and free amino acids (FAA, 75.27%). However, these negative effects of salinity stress were reversed mainly in rice plants after PM application. PM application (9%) remained the most effective and significantly increased growth, yield, TSP, FAA, accumulation of soluble sugars, proline, K+, and activity of antioxidant enzymes, namely, ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POD). Thus, these findings suggest a PM-mediated eco-friendly strategy for salinity alleviation in agricultural soil could be useful for plant growth and productivity in saline soils.
Rapid and Synchronous Breeding of Cytoplasmic Male Sterile and Maintainer Line Through Mitochondrial DNA Rearrangement Using Doubled Haploid Inducer in Brassica napus
Authors: Zhang Wei , Shi Haoran , Zhou Ying , Liang Xingyu , Luo Xuan , Xiao Chaowen , Li Yun , Xu Peizhou , Wang Jisheng , Gong Wanzhuo , Zou Qiong , Tao Lanrong , Kang Zeming , Tang Rong , Li Zhuang , Yang Jin , Fu Shaohong
Published: April 2022

When homozygously fertile plants were induced using doubled haploid (DH) induction lines Y3380 and Y3560, the morphology of the induced F1 generation was basically consistent with the female parent, but the fertility was separated, showing characteristics similar to cytoplasmic male sterile (CMS) and maintainer lines. In this study, the morphology, fertility, ploidy, and cytoplasm genotype of the induced progeny were identified, and the results showed that the sterile progeny was polima cytoplasm sterile (pol CMS) and the fertile progeny was nap cytoplasm. The molecular marker and test-cross experimental results showed that the fertile progeny did not carry the restorer gene of pol CMS and the genetic distance between the female parent and the offspring was 0.002. This suggested that those inductions which produced sterile and fertile progeny were coordinated to CMS and maintainer lines. Through the co-linearity analysis of the mitochondrial DNA (mtDNA), it was found that the rearrangement of mtDNA by DH induction was the key factor that caused the transformation of fertility (nap) into sterility (pol). Also, when heterozygous females were induced with DH induction lines, the induction F2 generation also showed the segregation of fertile and sterile lines, and the genetic distance between sterile and fertile lines was approximately 0.075. Therefore, the induction line can induce different types of female parents, and the breeding of the sterile line and the maintainer line can be achieved through the rapid synchronization of sister crosses and self-crosses. The induction of DH inducer in B. napus can provide a new model for the innovation of germplasm resources and open up a new way for its application.
Potassium Deficiency in Rice Aggravates Sarocladium oryzae Infection and Ultimately Leads to Alterations in Endophyte Communities and Suppression of Nutrient Uptake
Authors: Zhang Jianglin , Lu Zhifeng , Cong Rihuan , Ren Tao , Lu Jianwei , Li Xiaokun
Published: April 2022

Sheath rot disease is an emerging fungal disease in rice, whose infection causes severe yield loss. Sarocladium oryzae (S. oryzae) is the major causal agent. Previous study has demonstrated that rice deficiency in potassium (K) aggravates S. oryzae infection. However, the effects of S. oryzae infection on the nutrient-uptake process, endophyte communities, and hormone level of host plant under K-deficiency condition remain unclear, the mechanism of K mediated S. oryzae infection needs to be further study. The present study analyzed alterations in the endophytic community and nutrient-uptake process of host plants through an exogenous inoculation of S. oryzae in pot and hydroponics experiments. S. oryzae infection sharply increased the relative abundance of Ascomycota and decreased the Shannon and Simpson index of the endophytic community. Compared with the K-sufficient rice infected with S. oryzae, K-starved rice infected with S. oryzae (−K + I) increased the relative abundance of Ascomycota in leaf sheaths by 52.3%. Likewise, the −K + I treatment significantly decreased the Shannon and Simpson indexes by 27.7 and 25.0%, respectively. Sufficient K supply increased the relative abundance of Pseudomonas spp. in the host plant. S. oryzae infection profoundly inhibited the nutrient uptake of the host plant. The accumulation of oleic acid and linoleic acid in diseased rice decreased the biosynthesis of jasmonic acid (JA), and the content of JA was lowest in the −K + I treatment, which suppressed K+ uptake. These results emphasize the importance of K in resistance to S. oryzae infection by modulating endophyte community diversity and enhancing the nutrient-uptake capacity of the host plant.
Raman Spectroscopy and Machine Learning for Agricultural Applications: Chemometric Assessment of Spectroscopic Signatures of Plants as the Essential Step Toward Digital Farming
Authors: Farber Charles , Kurouski Dmitry
Published: April 2022

A growing body of evidence suggests that Raman spectroscopy (RS) can be used for diagnostics of plant biotic and abiotic stresses. RS can be also utilized for identification of plant species and their varieties, as well as assessment of the nutritional content and commercial values of seeds. The power of RS in such cases to a large extent depends on chemometric analyses of spectra. In this work, we critically discuss three major approaches that can be used for advanced analyses of spectroscopic data: summary statistics, statistical testing and chemometric classification. On the example of Raman spectra collected from roses, we demonstrate the outcomes and the potential of all three types of spectral analyses. We anticipate that our findings will help to design the most optimal spectral processing and preprocessing that is required to achieved the desired results. We also expect that reported collection of results will be useful to all researchers who work on spectroscopic analyses of plant specimens.
Meeting the Challenges of Chemical and Biological Weapons: Strengthening the Chemical and Biological Disarmament and Non-proliferation Regimes
Authors: Edwards Brett , Novossiolova Tatyana , Crowley Michael , Whitby Simon , Dando Malcolm , Shang Lijun
Published: April 2022

In this report, we identify some of the key technical and political challenges currently facing the broader Chemical and Biological Weapon (CBW) regime- with a particular emphasis on major forthcoming diplomatic meetings. Most significantly the Ninth Review Conference of the Biological and Toxins Weapons Convention (1972) (BTWC) which will take place in 2022 and preparations for the Fifth Review Conference of the Chemical Weapons Convention (1993) (CWC), expected in 2023. This report is an output of an ongoing project, designed to stimulate thinking and discussion about these issues, within relevant stakeholder communities. The report provides an introduction to this issue area for the general reader before surveying key issues and developing a series of practical policy suggestions for further consideration.
The Influence of Sense of Place on Elementary School Students’ Creativity During the COVID-19 Pandemic: The Mediating and Buffering Effects of Psychological Resilience
Authors: Xu Yanhua , Wang Qiaoling , Zhang Dongmei , Lin Peiying
Published: April 2022

Purpose

To understand the relationship between sense of place and creativity and the mechanisms that affect this relationship, the researchers constructed a mediation model to examine the effect of sense of place on creativity and the mediating role of psychological resilience in elementary school students during the COVID-19 pandemic.

Methods

A total of 1,711 students in an elementary school in Beijing, China, were surveyed using Chinese-language versions of the Sense of Place Scale, the Psychological Resilience Scale-Short Version, and the Innovative Behavior Inventory. SPSS (version 23) and PROCESS plug-in (version 3.3) were used for correlation and mediation analyses.

Results

(1) Correlation analysis revealed that sense of place was positively related to psychological resilience (r = 0.445, p < 0.01) and creativity (r = 0.590, p < 0.01). (2) Psychological resilience was also positively correlated with creativity (r = 0.625, p < 0.01). (3) Further, after controlling for gender and grade level, it was found that sense of place directly predicted creativity and that sense of place also indirectly predicted creativity through psychological resilience. The direct effect (0.45) and the mediating effect (0.23) accounted for 65.95 and 34.05% of the total effect, respectively.

Conclusion

The results demonstrate that sense of place is a positive predictor of creativity and can play a facilitating role to some extent. Moreover, psychological resilience is a mediating factor, acting as a buffer between sense of place and creativity. These results contribute to a more comprehensive understanding of the mechanisms influencing creativity.
An Assessment of China’s Digital Trade Development and Influencing Factors
Authors: Hu Yue , Zhou Han Qian , Yan Bin , Zou Zhou , Li Yu’an
Published: April 2022

The pattern and scale of commerce worldwide have been greatly transformed by the Fourth Industrial Revolution and technological advancement; digital trade has become the primary form of trade in the digital economy. On the basis of information network infrastructure, information technology level, digital industrialization level, and industrial digitalization level, this study establishes a comprehensive assessment system that applies an entropy–TOPSIS model to evaluate digital trade development level in China. The results indicate that digital trade in China was steadily growing between 2010 and 2019. A principal component analysis is conducted to identify factors affecting the digital trade development level in China. The analysis results suggest that Internet development, population income, industrial structure, payment convenience level, fixed asset investment, online transaction scale, and economic development all have positive effects on the digital trade development level in China, with payment convenience level having the greatest influence. By contrast, state intervention and degree of dependence on foreign trade have a negative effect on digital trade development.
Awareness of Social Influences on Eating Is Dependent on Familiarity With Imagined Dining Partners and Type of Eating Occasion
Authors: Higgs Suzanne , Bouguettaya Ayoub , Ruddock Helen
Published: April 2022

When eating with strangers, people tend to eat less than they would when eating alone (social inhibition of eating), whereas they tend to eat more with family and friends (social facilitation of eating). To assess awareness of the social inhibition and facilitation of eating we conducted two online studies (Study 1:N = 481; Study 2:N = 485). In Study 1, participants imagined a dining scenario and indicated whether they would eat the same, more, or less when eating with someone who was more or less familiar to them (friend; family member; acquaintance; stranger) compared with when eating alone. Results from Study 1 indicated that participants imagined eating special meals with friends/family and so, in Study 2, another group of participants made the same predictions as for Study 1, but they did so for special and regular meals. In Study 1, a significant majority of participants said that they would “eat less” with a stranger/acquaintance. A similar proportion of participants said that they would “eat the same” or “eat more” when with a friend/family member and significantly fewer participants said that they would “eat less” with a friend/family member. In Study 2, the majority of participants said that they would “eat less” with a stranger across both special and regular meals. For meals with an acquaintance, the majority of participants reported that they would “eat the same” during regular meals, while for special meals, equal numbers said that they would “eat the same” as would “eat less.” The majority of participants indicated that they would “eat more” during a special meal with a friend/family member. However, for regular meals with a friend/family member, a significant majority of participants said that they would “eat the same.” Hence, participants acknowledge the social inhibition of intake and the social facilitation of eating at special meals, but they are either unwilling or unable to acknowledge that they would eat more with a friend/family member at a regular meal compared with eating alone. Raising awareness that eating with friends/family is associated with greater intake at regular meals may be helpful for individuals who are trying to manage their intake.
Teachers’ Commitment and Self-Efficacy as Predictors of Work Engagement and Well-Being
Authors: Shu Kunyao
Published: April 2022

In the field of language-education psychology, the psychology of educators was not at the center of attention to some extent, despite the immense attention given to the psychology of students. Recently, positive psychology has become very important as it puts more emphasis on the constructive dimensions of life and it regards individual well-being as a major problem for individuals’ achievement. Since the core of an instructing institution is the educators, their well-being must be prioritized in the academic circumstances to both improve students’ skills and to motivate and inspire participation and involvement in the class. Moreover, teacher engagement is viewed as another significant factor in this realm that refers to people’s outlook toward their job, impacting their emotional attendance and engagement during their presentation. Also, there is an inner force that pushes educators to put more time and strength in maintaining participation in the school that is called educator commitment that is determined on evolving the school builds an emotive connection between educators and schools. It eventually encourages educators to improve their instructing careers and find ways to create a successful educational setting that would enable learners to attain their goals. In addition, teacher self-efficacy, including educators’ convictions in their skill to successfully manage assignments, responsibilities, and difficulties associated with their expert activity, has an important function in affecting key scholastic results in the career setting. By taking the aforementioned constructs, this review of literature provides implications for academics, teachers, and policymakers in search of better considering the functions of commitment and self-efficacy on their work engagement and well-being.
Visuomanual Vertical Prism Adaptation: Aftereffects on Visuospatial and Auditory Frequency Representations
Authors: Bonnet Clémence , Poulin-Charronnat Bénédicte , Ardonceau Vincent , Sirandré Cyril , Bard Patrick , Michel Carine
Published: April 2022

Sensorimotor aftereffects have been widely studied after lateral prism adaptation but not after vertical prism adaptation. It is thus well-known that lateral prism adaptation produces aftereffects on visuospatial representation and, recently, on auditory perception. This study aimed to explore the sensorimotor after-effects of vertical prism adaptation as well as its aftereffects on vertical visuospatial representation (Experiment 1) and on auditory frequency representation (Experiment 2). The experimental procedure was similar in both experiments: before and after prism adaptation to an upward or a downward optical deviation, healthy young participants performed an visual open-loop pointing task and a visual (Experiment 1) or an auditory (Experiment 2) perceptual bisection task. In the visual task, the participants had to indicate if they perceived the bisection as higher or lower than the true center of a line. In the auditory task, the participants had to indicate if they perceived the target auditory frequency closer to the low or the high limit of an auditory interval. For sensorimotor aftereffects, pointing errors were computed by means of a vertical touchscreen. For the perceptual bisection task, we measured the percentage of “down” (Experiment 1) or “low” responses (Experiment 2), and we computed the visual (Experiment 1) or the auditory (Experiment 2) subjective center for each participant. Statistical analyses were carried out separately for each optical deviation in each experiment. Sensorimotor aftereffects were observed in both experiments, in the opposite direction to the optical deviation (all ps < 0.01). No significant aftereffects occurred on visuospatial representation (all ps > 0.5), whereas the percentage of “low” responses and the auditory subjective center significantly increased after adaptation to a downward optical deviation (all ps < 0.05). Unlike lateral prism adaptation aftereffects that have been previously shown in both visuospatial horizontal representation and auditory frequency representation, aftereffects of vertical prism adaptation occurred in the auditory frequency representation but not in the vertical visuospatial representation. These results suggest that both vertical and lateral prism adaptations share a common substrate dedicated to the auditory modality (probably the temporal cortex), and that vertical adaptation does not act on the neural substrate of vertical visuospatial representation.
Physical Activity Adherence Related to Body Composition and Physical Fitness in Spanish Older Adults: 8 Years-Longitudinal EXERNET-Study
Authors: Jiménez-Zazo Fabio , Hernández-Martínez Antonio , Romero-Blanco Cristina , Cabanillas Esther , Pérez-Gómez Jorge , Ara Ignacio , Casajús José A. , Vicente-Rodríguez Germán , Gesteiro Eva , González-Gross Marcela , Aznar Susana
Published: April 2022

Introduction

The multiple benefits of physical activity (PA) compared to the current lack of adherence to this behavior in older people makes it necessary to identify the factors related to its long-term dropout, therefore, the aim of this study was: (i) to study the evolution of behavior change for PA through stages of change (SoC) after 8 years and, to identify the associated factors related to the worsening of SoC for PA.

Methods

A total of 714 Spanish older adults (>65 years) participated in the Longitudinal elderly EXERNET study 8 years follow-up. SoC for PA from Transtheoretical model of behavior change (TTM), body composition [BMI, fat mass, lean mass, waist circumference (WC), and hip circumference (HC)] and physical fitness (EXERNET battery fitness tests) were recorded. A multivariate binary logistic regression model was used to identify the potential predictive factors of worsening SoC for PA.

Results

Three hundred and sixty participants (50.4%) worsening their SoC for PA after 8 years. Predictors factors associated with worsening of SoC were: HC (OR = 1.022; 95% CI: 1.001, 1.044), Lower body strength (OR = 0.940; 95% CI: 0.887, 0.997) and aerobic endurance at the 6 min-walk test (OR = 0.997; 95% CI: 0.995, 0.999).

Conclusion

This study highlights that we need to identify adults with high HC, lower body strength and lower aerobic endurance to design a tailored PA program. Identifying the determinants of losing adherence to PA will help in the creation, design, and evaluation of exercise programs with the goal of reducing older adults’ drop-outs rates.
Self-Efficacy Mechanism in Farm Tourism Microentrepreneurship
Authors: Ferreira Bruno , Morais Duarte B. , Jakes Susan , Brothers Gene , Brookins Craig
Published: April 2022

Tourism microentrepreneurship is an important farm diversification strategy, also contributing to the competitiveness of the destination. However, psychological and structural constraints seem to hold back farmers when it comes to starting or expanding tourism operations. We argue that social capital derived from farmers’ social networks affords sources of entrepreneurial self-efficacy, which boosts entrepreneurial intention. Analysis of survey data from 207 farmers in North Carolina revealed an adequate SEM model fit and strong significant relationships between bridging social capital and tourism microentrepreneurial self-efficacy. Internal self-efficacy factors were strongly and significantly associated with entrepreneurial intention. Triangulation with qualitative data from participatory-action research reinforced the importance of informal networking processes to model entrepreneurial behavior that boosts self-efficacy and reaffirms microentrepreneurial intentions. However, external self-efficacy was not significantly associated with farmers’ entrepreneurial intentions, which may be attributable to ambiguous agritourism policy.
Toward an Ecological Understanding of Transnational Chinese Language Teachers’ Professional Wellbeing in the United Kingdom
Authors: Wang Isobel Kai-Hui
Published: April 2022

Given the lack of reports of Chinese language teachers’ wellbeing in the literature, this study aims to investigate the professional wellbeing of eight teachers of Chinese as a foreign language in the United Kingdom based on in-depth, semi-structured qualitative interviews. Interview data provided a rich picture of the rewarding aspects and challenges that they experienced in their professional lives. The main findings revealed the complex interplay of their professional wellbeing and different levels of ecology (i.e., cultural, institutional, classroom, and personal). The study also identified the specific strategies that the teachers deployed for (1) coping with work-related stress and for (2) maintaining wellbeing alongside professional productivity. The paper calls for further research to apply a close-up lens to the wellbeing of foreign language teachers across timescales. The implications for transnational language teachers’ mentoring interventions are discussed.
Formation Mechanism and Dynamic Evolution Laws About Unsafe Behavior of New Generation of Construction Workers Based on China’s Construction Industry: Application of Grounded Theory and System Dynamics
Authors: Ni Guodong , Lv Lei , Wang Shaobo , Miao Xinyue , Fang Yaqi , Liu Qing
Published: April 2022

Construction workers’ unsafe behavior is a major cause of safety accidents and injuries, therefore, a profound understanding of the formation process and evolution laws about construction workers’ unsafe behavior is conducive to taking measures to prevent incidents. At present, the new generation of construction workers (NGCWs) born after 1980 are gradually becoming the main force at construction sites in China. Given that generational differences of construction workers can cause the discrepancies in their thoughts and attitudes when engaging in safety-related activities, this study aims to investigate the formation mechanism and dynamic evolution laws about NGCWs’ unsafe behavior based on the context of China’s construction industry. From the perspective of behavior motivation, in-depth semi-structured interviews with 18 NGCWs and 7 grassroots managers were conducted, and data analysis followed a three-step coding process based on grounded theory. Through continuous comparison, abstraction and analysis, the stimulus-organism-response theory was introduced and expanded to construct a three-stage formation mechanism model. On this basis, the causal diagram and stock flow diagram were developed based on system dynamics principles to reflect the dynamic feedback relationships of the factors in the static formation mechanism model, and simulation was carried out using Vensim PLE software. The results show that three types of internal needs and three types of external incentives stimulate corresponding motivations for NGCWs’ unsafe behavior. Two types of individual factors, five types of situational factors and behavior result play an influencing role in the decision-making process of externalizing motivation into behavior. Under the synergistic effect of multiple factors, the level of unsafe behavior displays a downward trend, and the rate of decrease is slow first and then fast. Furthermore, among individual factors and situational factors, safety awareness and safety management system have the most significant effect on the level of unsafe behavior, while situational factors play a more obvious role. The findings can provide theoretical support and practical references to China’s construction companies and government departments for the purpose of improving NGCWs’ unsafe behavior.