bluuebunny/crossref_metadata_2025 huggingface dataset:
An Interior Regularity Property for the Solution to a Linear Elliptic System with Singular Coefficients in the Lower-Order Term
Authors: Radice Teresa
Published: February 2025

This paper deals with the interior higher differentiability of the solution u to the Dirichlet problem related to system −div(A(x)Du)+B(x,u)=f on a bounded Lipschitz domain Ω in Rn. The matrix A(x) lies in the John and Nirenberg space BMO. The lower-order term B(x,u) is controlled with respect to the spatial variable by a function b(x) belonging to the Marcinkiewicz space Ln,∞. The novelty here is the presence of a singular coefficient in the lower-order term.
Uncertain Numbers
Authors: Yue Peng
Published: February 2025

This work presents a mathematical framework based on uncertain numbers to address the inherent uncertainty in nonlinear systems, a challenge that traditional mathematical frameworks often struggle to fully capture. By establishing five axioms, a formal system of uncertain numbers is developed and embedded within set theory, providing a comprehensive characterization of uncertainty. This framework allows phenomena such as infinity and singularities to be treated as uncertain numbers, offering a mathematically rigorous analytical approach. Subsequently, an algebraic structure for uncertain numbers is constructed, defining fundamental operations such as addition, subtraction, multiplication, and division. The framework is compatible with existing mathematical paradigms, including complex numbers, fuzzy numbers, and probability theory, thereby forming a unified theoretical structure for quantifying and analyzing uncertainty. This advancement not only provides new avenues for research in mathematics and physics but also holds significant practical value, particularly in improving numerical methods to address singularity problems and optimizing nonconvex optimization algorithms. Additionally, the anti-integral-saturation technique, widely applied in control science, is rigorously derived within this framework. These applications highlight the utility and reliability of the uncertain number framework in both theoretical and practical domains.
Convergence Analysis for Differentially Private Federated Averaging in Heterogeneous Settings
Authors: Li Yiwei , Wang Shuai , Wu Qilong
Published: February 2025

Federated learning (FL) has emerged as a prominent approach for distributed machine learning, enabling collaborative model training while preserving data privacy. However, the presence of non-i.i.d. data and the need for robust privacy protection introduce significant challenges in theoretically analyzing the performance of FL algorithms. In this paper, we present novel theoretical analysis on typical differentially private federated averaging (DP-FedAvg) by judiciously considering the impact of non-i.i.d. data on convergence and privacy guarantees. Our contributions are threefold: (i) We introduce a theoretical framework for analyzing the convergence of DP-FedAvg algorithm by considering different client sampling and data sampling strategies, privacy amplification and non-i.i.d. data. (ii) We explore the privacy–utility tradeoff and demonstrate how client strategies interact with differential privacy to affect learning performance. (iii) We provide extensive experimental validation using real-world datasets to verify our theoretical findings.
A Semismooth Newton-Based Augmented Lagrangian Algorithm for the Generalized Convex Nearly Isotonic Regression Problem
Authors: Xu Yanmei , Lin Lanyu , Liu Yong-Jin
Published: February 2025

The generalized convex nearly isotonic regression problem addresses a least squares regression model that incorporates both sparsity and monotonicity constraints on the regression coefficients. In this paper, we introduce an efficient semismooth Newton-based augmented Lagrangian (Ssnal) algorithm to solve this problem. We demonstrate that, under reasonable assumptions, the Ssnal algorithm achieves global convergence and exhibits a linear convergence rate. Computationally, we derive the generalized Jacobian matrix associated with the proximal mapping of the generalized convex nearly isotonic regression regularizer and leverage the second-order sparsity when applying the semismooth Newton method to the subproblems in the Ssnal algorithm. Numerical experiments conducted on both synthetic and real datasets clearly demonstrate that our algorithm significantly outperforms first-order methods in terms of efficiency and robustness.
Antioxidant Peptides from Miiuy Croaker Swim Bladders: Ameliorating Effect and Mechanism in NAFLD Cell Model through Regulation of Hypolipidemic and Antioxidant Capacity
Authors: Wang Yu-Mei , Ge Ming-Xue , Ran Su-Zhen , Pan Xin , Chi Chang-Feng , Wang Bin
Published: February 2025

In this work, the hypolipidemic and antioxidative capacity of FSGLR (S7) and GIEWA (S10) from miiuy croaker swim bladders was explored systematically in an oleic acid (OA)-induced nonalcoholic fatty liver disease (NAFLD) model of HepG2 cells. Moreover, the hypolipidemic activity of S7 and S10 and their antioxidative abilities were preliminarily investigated in combination with molecular docking technology. The results indicated that S7 and S10 could decrease the amount of lipid accumulation and the content of triglycerides (TG) and total cholesterol (TC) in the OA-induced NAFLD cell model in a dose-dependent manner. In addition, S7 and S10 exhibited better bile salt binding, pancreatic lipase (PL) inhibition, and cholesterol esterase (CE) inhibition capacities. The hypolipidemic mechanisms of S7 and S10 were connected with the downregulation of the mRNA expression levels of adipogenic factors, including sterol-regulatory element-binding protein-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), sterol-regulatory element-binding protein (SREBP)-2, hydroxymethylglutaryl-CoA reductase (HMGR), and fatty acid synthase (FAS) (p < 0.01), and the upregulation of the mRNA expression of β-oxidation-related factors, including carnitine palmitoyltransferase 1 (CPT-1), acyl-CoA oxidase 1 (ACOX-1), and peroxisome proliferator-activated receptor α (PPARα). Moreover, FSGLR (S7) and GIEWA (S10) could significantly protect HepG2 cells against OA-induced oxidative damage, and their antioxidant mechanisms were related to the increased activity of intracellular antioxidant proteases (superoxide dismutase, SOD; glutathione peroxidase, GSH-PX; catalase, CAT) to remove excess reactive oxygen species (ROS) and decrease the production of malondialdehyde (MDA). The presented findings indicate that the hypolipidemic and antioxidant functions and mechanisms of S7 and S10 could make them potential hypolipidemic and antioxidant candidates for the treatment of NAFLD.
Refractive Alterations in Marfan Syndrome: A Narrative Review
Authors: Vakalopoulos Dionysios G. , Lampsas Stamatios , Chatzea Marina S. , Togka Konstantina A. , Tsagkogiannis Vasileios , Mitsopoulou Dimitra , Lalou Lida , Lampsa Aikaterini , Katsimpras Marios , Petrou Petros , Kymionis George D.
Published: February 2025

Marfan syndrome (MFS) is a genetic disorder that affects the connective tissue in several systems, with ocular, cardiovascular, and skeletal system manifestations. Its ocular manifestations include ectopia lentis (EL), myopia, astigmatism, and corneal abnormalities. This review examines refractive alterations related to MFS such as EL, microspherophakia, lens coloboma, altered corneal biomechanics (flattening, thinning, and astigmatism), and myopia and their impact on visual acuity. The pathogenesis of these manifestations stems from mutations in the FBN1 gene (encoding fibrillin-1). Moreover, the current medical and surgical management strategies for MFS-related refractive errors, including optical correction (eyeglasses, contact lenses, etc.), and surgical interventions like lensectomy, intraocular lens (IOL) implantation (anterior chamber, posterior chamber, scleral-fixated, iris-fixated), and the use of capsular tension rings/segments are further discussed. Considering the likelihood of underdiagnosing and underestimating ocular involvement in MFS, this updated review highlights the critical need to identify and address these refractive issues to enhance the visual outcomes for those affected.
Exercise Training and Cardiovascular Risk Factors in Males with Overweight or Obesity: A Systematic Review of Randomized Controlled Trials
Authors: Pourmotahari Aynaz , Shahrbanian Shahnaz , Supriya Rashmi , Saeidi Ayoub
Published: February 2025

Background and Objectives: Obesity is a significant risk factor for cardiovascular disease. Physical exercise has been established as an effective intervention for reducing cardiovascular mortality. This systematic review aimed to investigate the effects of various exercise modalities on cardiovascular risk factors in men with obesity. Materials and Methods: This review included randomized controlled trials (RCTs) published between 2005 and November 2023. Studies were eligible if they assessed the impact of exercise interventions on blood pressure (BP), lipid profiles, and glucose/insulin regulation in males aged 18 years or older with a body mass index (BMI) of 25 kg/m2 or greater. The quality of included studies was evaluated using the Physiotherapy Evidence Database (PEDro) scale. Results: A total of 25 RCTs were included in the analysis, with PEDro scores ranging from 3 to 7. High-intensity interval training (HIIT) was associated with significant reductions in BP, with an average decrease of 12.5 mmHg. However, the magnitude of BP reduction varied across studies, likely due to differences in training protocols and study quality. Resistance training (RT) was associated with modest improvements in glycemic control, with some studies reporting a reduction in fasting blood glucose levels by 5–10%. Combined exercise (CT) programs, which included both aerobic and resistance training, as well as aerobic exercise alone, were shown to improve lipid profiles, with some studies showing reductions in total cholesterol levels ranging from 10–15%. However, the findings were inconsistent, highlighting the need for further research to better understand the potential benefits and optimal exercise regimens. Conclusions: This systematic review supports the potential of exercise training in reducing cardiovascular risk factors in men with obesity, though the effectiveness varies depends on the type of exercise. Combined exercise programs have shown promise for lipid profiles, but further research is needed to confirm these effects. HIIT has been linked to BP reductions in some studies, although the results are inconsistent and depend on protocols and study quality. The limitations in study quality may impact the reliability of these findings.
Conformation and Membrane Topology of the N-Terminal Ectodomain of Influenza A M2 Protein
Authors: Roeke Kyra C. , Howard Kathleen P.
Published: February 2025

The N-terminal ectodomain of the influenza A M2 protein is a target for universal influenza vaccine development and novel antiviral strategies. Despite the significance of this domain, it is poorly understood and most structural studies of the M2 protein have disregarded the N-terminal ectodomain in their analyses. Here, we report conformational properties and describe insights into the membrane topology of sites along the N-terminal ectodomain. Full-length M2 protein is embedded in lipid bilayer nanodiscs and studied using site-directed spin labeling electron paramagnetic resonance spectroscopy. Results are consistent with a turn in the middle of the ectodomain that changes in proximity to the membrane surface upon the addition of cholesterol or the antiviral drug rimantadine. Similarly to other domains of M2 protein, lineshape analysis suggests that the N-terminal ectodomain can adopt multiple conformations.
Silica-Nanocoated Membranes with Enhanced Stability and Antifouling Performance for Oil-Water Emulsion Separation
Authors: Zhu Mengfan , Huang Chengqian , Mao Yu
Published: February 2025

Despite the potential of glass fiber (GF) membranes for oil-water emulsion separations, efficient surface modification methods to enhance fouling resistance while preserving membrane performance and stability remain lacking. We report a silica nanocoating method to modify GF membranes through a vapor deposition method. The high smoothness (<1 nm r.m.s.) and high conformality of the vapor-deposited silica nanocoatings enabled the preservation of membrane microstructure and permeability, which, combined with the enhanced surface hydrophilicity, led to an oil rejection rate exceeding 99% and more than a 40% improvement in permeate flux in oil-water emulsion separations. Furthermore, the silica nanocoatings provided the membranes with excellent wet strength and stability against organic solvents, strong acids, oxidants, boiling, and sonication. The silica-nanocoated membrane demonstrated enhanced fouling resistance, achieving flux recovery higher than 75% during repeated oil-water emulsion separations and bovine serum albumin and humic acid fouling tests.
Molecular Evolutionary Analyses of the RNA-Dependent RNA Polymerase (RdRp) Region and VP1 Gene in Sapovirus GI.1 and GI.2
Authors: Mizukoshi Fuminori , Kimura Ryusuke , Shirai Tatsuya , Hirata-Saito Asumi , Hiraishi Eri , Murakami Kosuke , Doan Yen Hai , Tsukagoshi Hiroyuki , Saruki Nobuhiro , Tsugawa Takeshi , Kidera Kana , Suzuki Yoshiyuki , Sakon Naomi , Katayama Kazuhiko , Kageyama Tsutomu , Ryo Akihide , Kimura Hirokazu
Published: February 2025

Human sapovirus (HuSaV) is a significant cause of gastroenteritis. This study aims to analyze the evolutionary dynamics of the RNA-dependent RNA polymerase (RdRp) and capsid (VP1) genes of the HuSaV GI.1 and GI.2 genotypes between 1976 and 2020. Using bioinformatics tools such as the Bayesian phylogenetics software BEAST 2 package (v.2.7.6), we constructed time-scale evolutionary trees based on the gene sequences. Most of the recent common ancestors (MRCAs) of the RdRp region and VP1 gene in the present HuSaV GI.1 diverged around 1930 and 1933, respectively. The trees of the HuSaV GI.1 RdRp region and VP1 gene were divided into two clusters. Further, the MRCAs of the RdRp region and VP1 gene in HuSaV GI.2 diverged in 1960 and 1943, respectively. The evolutionary rates were higher for VP1 gene in HuSaV GI.1 than that in HuSaV GI.2, furthermore, were higher in GI.1 Cluster B than GI.1 Cluster A. In addition, a steep increase was observed in the time-scaled genome population size of the HuSaV GI.1 Cluster B. These results indicate that the HuSaV GI.1 Cluster B may be evolving more actively than other genotypes. The conformational B-cell epitopes were predicted with a higher probability in RdRp for GI.1 and in VP1 for GI.2, respectively. These results suggest that the RdRp region and VP1 gene in HuSaV GI.1 and GI.2 evolved uniquely. These findings suggest unique evolutionary patterns in the RdRp region and VP1 gene of HuSaV GI.1 and GI.2, emphasizing the need for a ‘One Health’ approach to better understand and combat this pathogen.
Adaptive Resistance of Staphylococcus aureus to Cefquinome Sulfate in an In Vitro Pharmacokinetic Model with Transcriptomic Insights
Authors: Hu Yue , Zhu Hao , Zhang Xingbo , Wu Yuhui , Li Jingtao , Li Nan , Cai Zhanbo , Yang Yuhui
Published: February 2025

Cefquinome sulfate has a strong killing effect against Staphylococcus aureus (S. aureus), but bacterial resistance has become increasingly widespread. Experiments were conducted to investigate the pattern of adaptive resistance of S. aureus to cefquinome sulfate under different dosage regimens by using pharmacokinetic-pharmacodynamics (PK-PD) modeling, and the adaptive-resistant bacteria in different states were screened and subjected to transcriptomic sequencing. The results showed that the minimum inhibitory concentration of Staphylococcus aureus under the action of cefquinome sulfate was 0.5 μg/mL, the anti-mutation concentration was 1.6 μg/mL, and the mutation selection window range was 0.5~1.6 μg/mL. In the in vitro pharmacokinetic model to simulate different dosing regimens in the animal body, there are certain rules for the emergence of adaptive drug-resistant bacteria: the intensity of bacterial resistance gradually increased with culture time, and the order of emergence was tolerant bacteria (TO) followed by persistent bacteria (PE) and finally resistant bacteria (RE). The sequence reflected the evolution of adaptive drug resistance. Transcriptome Gene Ontology (GO) analysis revealed that differentially expressed genes were involved in cellular respiration, energy derivation by oxidation of organic compounds, and oxidation–reduction processes. The differentially expressed genes identified functioned in the synthesis of cell membranes, cytoplasm, and intracellular parts. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis found that 65 genes were differentially expressed after cefquinome sulfate treatment, of which 35 genes were significantly upregulated and 30 genes were significantly downregulated. Five genes, sdhB, sdhA, pdhA, lpdA, and sucC, may be involved in network regulation. This study revealed the cross-regulation of multiple metabolic pathway networks and the targets of network regulation of S. aureus to produce adaptive drug resistance. The results will provide guidance for clinical drug use in animals infected with S. aureus.
An Advanced Approach for Geometallurgical Modeling Applied to Bauxite Mines
Authors: Rodovalho Edmo , Lima José , Campos Pedro , Casagrande Pedro , Mazzinghy Douglas
Published: February 2025

Geometallurgy is an approach that integrates geology, mining, processing, and environmental areas, aiming to increase knowledge of the deposit and reduce risks in mining projects and/or operations. Growing changes in economic and operational scenarios require the development of robust models for metallurgical responses. Typically, the population of geometallurgical variables is small, and there are restrictions on applying geostatistical techniques, such as Ordinary Kriging, since recoveries are considered non-additive variables. This study used multiple linear regression to develop a geometallurgical model for a bauxite mine. The developed models enabled reconciliation with real results, and this application for bauxite mining represents a novelty in the literature. The model estimated the mass recovery in the coarse fraction with an accuracy greater than 97%. Additionally, the geometallurgical model developed for Mineração Rio do Norte (MRN) allows predictability and mapping potential product quality deviations.
New Benzothiazole–Monoterpenoid Hybrids as Multifunctional Molecules with Potential Applications in Cosmetics
Authors: Kirkova Desislava , Stremski Yordan , Bachvarova Maria , Todorova Mina , Goranov Bogdan , Statkova-Abeghe Stela , Docheva Margarita
Published: February 2025

The Thymus vulgaris and Origanum vulgare essential oils (contained thymol and carvacrol in a range of 35–80%) are used in various products in the fields of medicine, cosmetics, and foods. Molecular hybridization between benzothiazole (BT) and phenolic monoterpenoids is a promising method for the development of biologically active compounds. New benzothiazole–monoterpenoid hybrids were synthesized through a regioselective α-amidoalkylation reaction of thymol and carvacrol with high yields (70–96%). This approach is both simple and cost-effective, employing easily accessible and inexpensive reagents to produce target molecules. The structure of the synthesized compounds was characterized spectrally using 1H-, 13C-NMR, FT-IR, and HRMS data. The newly obtained compounds are structural analogues of the UVB filter PBSA, which is used in cosmetics. The spectral properties of the aromatic products thymol hybrid (2-(4-hydroxy-5-isopropyl-2-methylphenyl)benzo[d]thiazole) and carvacrol hybrid (2-(4-hydroxy-2-isopropyl-5-methylphenyl)benzo[d]thiazole) were successfully examined, using a validated spectrophotometric method. SPF values varied from 31 to 36, compared to the PBSA (30), and were observed at concentrations of 1–0.25 mM. 2-Hydroxyphenylbenzothiazoles are known antimicrobial and antioxidant agents that have potential applications in the food industry and cosmetics as preservatives and antioxidants. In this context, antimicrobial activity of the hybrid compounds was evaluated using the agar diffusion method against E. coli, S. aureus, P. aeruginosa, and C. albicans. Compounds of methyl-2-(4-hydroxy-2-isopropyl-5-methylphenyl)benzo[d]thiazole-3(2H)-carboxylate containing carvacrol fragments showed high activity against Staphylococcus aureus ATCC 25923 (with 0.044 μmol content). The radical scavenging activity was determined using ABTS and DPPH assays, the highest activity was exhibited by the thymol hybrids ethyl-2-(4-hydroxy-5-isopropyl-2-methylphenyl)benzo[d]thiazole-3(2H)-carboxylate (IC50—133.70 ± 10 µM) and methyl-2-(4-hydroxy-5-isopropyl-2-methylphenyl)benzo[d]thiazole-3(2H)-carboxylate (IC50—157.50 ± 10 µM), defined by ABTS. The aromatic benzothiazole–monoterpenoid hybrids are classified using in silico analyses as non-mutagenic, with low toxicity, and they are non-irritating to the skin. These compounds were identified as new hit scaffolds for multifunctional molecules in cosmetics.
Halogen Bonding and Rearrangements in Complexes of N-Chlorosuccinimide with Halides
Authors: Hardin Maison , Zeller Matthias , Rosokha Sergiy V.
Published: February 2025

The role of halogen bonding (HaB) in the reactions of N-chlorosuccinimide (SimCl), a versatile reagent in organic synthesis, was investigated through experimental and computational analyses of its interactions with halides. The reactions of SimCl with Br− or I− resulted in the crystallization of HaB complexes of chloride with N-iodosuccinimide (SimI) or N-bromosuccinimide (SimBr). Computational analysis revealed that halogen rearrangements, which occurred even at −73 °C, were facilitated by halogen bonding. The dissociation of SimCl∙Y− (Y = I or Br) complexes into a Sim− + ClY pair (followed by the rotation and re-binding of the interhalogen molecules) bypassed the formation of the high-energy Sim− + Cl+ pair and drastically (about tenfold) reduced the dissociation energy of the N–Cl bond. Furthermore, while the dissociation energy of individual SimCl is higher (and its HaB is weaker) compared to that of SimI or SimBr, the dissociation of the N-Cl bond in SimCl∙Y− requires less energy than in the complexes of SimBr or SimI. The facile cleavage of such bonds in HaB complexes explains the high reactivity of SimCl and its effectiveness as a halogenating agent.
Shedding Light on the Antioxidant Activity of Bee Venom Using a 2,2-Diphenyl-1-Picrylhydrazyl Assay in a Detergent-Based Buffer
Authors: Orrù Alessandro , Pittau Barbara , Pettinau Francesca
Published: February 2025

Honeybee venom (HBV) is a complex mixture of proteins and enzymes used in traditional medicine to treat various ailments. HBV has multiple pharmacological effects, making it a promising therapeutic agent in several medical areas. In addition, HBV has many potential cosmetic applications as an anti-aging agent and for the treatment of various skin conditions. HBV’s antioxidant properties are also of great interest, as oxidative stress contributes to the onset and progression of many diseases. Several attempts have been made to assess HBV’s antioxidant activity, mainly using the DPPH assay. However, variability in experimental protocols and the lack of experimental details make the interpretation of results difficult. In this study, we aim to address the source of this variability by investigating the antioxidant activity of HBV in a detergent-based buffer across a range of pH values (from 3 to 7.5). We also analyze the contribution of melittin, the major component of HBV. Our results demonstrate that the DPPH radical scavenging activity of HBV is strongly influenced by the solvent used and by pH. Specifically, we show, for the first time, that HBV exhibits antioxidant activity under mildly acidic conditions, following a complex fast + slow reaction pattern. Interestingly, melittin contributes only partially to the total antioxidant activity of HBV. Overall, this work provides new insights into the antioxidant properties of HBV.
Phytochemical Profile and Biological Activities of the Endemic Thymbra nabateorum Occurring in AlUla County, NW Saudi Arabia
Authors: Miara Mohamed Djamel , Youssef Sami , Fendane Yassine , Boukcim Hassan , Almalki Sami D. , Rees Shauna K. , Lee Benjamin P. Y.-H. , Aldabaa Abdalsamad , Bendif Hamdi , Mohamed Ahmed H.
Published: February 2025

Thymbra nabateorum, a plant species from the Lamiaceae family, is endemic to the Nabatian region, which spans southern Jordan and northwestern Saudi Arabia. It is renowned for its traditional uses and rich phytochemical profile. This study aims to examine the phytochemical composition of T. nabateorum and evaluate its biological activities, including antioxidant capacity, cytotoxic effects on cancer cell lines, and enzyme inhibition relevant to diabetes and neurodegenerative diseases. The essential oil (EO) and methanol extract of T. nabateorum were analyzed using Gas Chromatography–Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC). Antioxidant activity was assessed using the DPPH radical scavenging assay. Cytotoxicity was evaluated against MDA-MB231 and LNCaP cancer cell lines using the MTT assay. Enzyme inhibition assays were conducted to determine the inhibitory effects on α-amylase, α-glucosidase, and butyrylcholinesterase. GC-MS analysis revealed thymol (82.30%) as the major component of the essential oil, while HPLC identified significant phenolic compounds in the methanol extract, including diosmin (118.75 mg/g) and hesperidin (22.18 mg/g). The DPPH assay demonstrated strong antioxidant activity, with the methanol extract showing an IC50 of 11.97 μg/mL for α-amylase and 31.99 μg/mL for α-glucosidase, indicating notable antidiabetic potential. Cytotoxicity tests revealed significant antiproliferative effects against both cancer cell lines, with lower IC50 values compared to standard treatments. T. nabateorum exhibits substantial antioxidant, cytotoxic, and enzyme inhibition activities, supporting its traditional medicinal uses. These findings provide a scientific basis for further research into its bioactive compounds and potential applications in modern pharmacology, particularly in developing natural therapeutic agents for oxidative stress-related diseases and cancer.
Exploring Bovine Serum Albumin (BSA) as a Facile Substrate for Testing of Osmolytes as Cosmetic Ingredients
Authors: Sinesi Giulia , Salvioni Lucia , Ronchi Elisabetta , Barbuzzi Elena Maria Gabriella , Prosperi Davide , Colombo Miriam , Giustra Marco Davide
Published: February 2025

Osmolytes, cellular compounds that maintain osmotic balance and integrity, are gaining attention in cosmetics for their ability to modulate protein folding and stability. Their incorporation into hair and skin care products enhances hydration and strengthens cellular structures. As the cosmetic industry seeks innovative and effective ingredients, osmolytes remain promising candidates for further advancing technologies. This study evaluates the protective effects of four osmolytes—betaine, erythritol, glycine, and isopentyldiol—on bovine serum albumin (BSA), as a substrate, under stressors such as UV irradiation, extreme temperatures, pH changes, surfactants, and oxidative stress. Protein stability was assessed via fluorescence emission. Only betaine at 0.50% w/w provided significant protection under oxidative stress, while glycine was the most effective osmolyte under basic and acidic pH and UVC radiation. Betaine showed potential in mitigating cold-induced stress but increased stress under UVA radiation, highlighting its condition-specific effects. The study emphasizes the need for comprehensive screening and synergistic testing of osmolytes to optimize cosmetic formulations for diverse stressors. Among the tested osmolytes, glycine is a promising candidate for skin and hair care products, though further research is needed. These findings provide a rapid, cost-effective method to evaluate osmolytes’ potential, offering valuable insights for cosmetic formulation.
Insights into Adsorption Behaviors of Multi-Component Shale Oil in Illite Nanopores Under Different Reservoir Conditions by Molecular Simulation
Authors: Zhang Lingtan , Tan Maojin , Liu Xuefeng , Lu Xiaoqing , Wang Qian , Wang Siyu , Tian Min , Wang Junjie
Published: February 2025

Clay pores are important storage spaces in shale oil reservoirs. Studying the adsorption behavior of shale oil in clay nanopores is of great significance for reserve assessment and exploitation. In this work, illite clay pore models and multi-component shale oil adsorption models considering light hydrocarbon correction are constructed for carrying out molecular dynamics simulation. We studied the adsorption behavior and characteristics of shale oil in illite pores, and analyzed the effects of reservoir environmental factors such as temperature, pressure and pore size on the adsorption behavior. The results show that in illite nanopores, shale oil can form multiple adsorption layers. The heavier the component, the stronger the interaction with the wall. The adsorption ratio of the component is closely related to the solid–liquid interaction and the molar fraction, which preliminarily reveals the reason why the heavy component content in the produced oil is considerable. The increase in temperature promotes the desorption of light and medium components, while the heavy components and dissolved gas are less affected; although the increase in pressure inhibits diffusion, the adsorption amount changes little, and only the light component increases slightly. This study deeply reveals the adsorption mechanism of shale oil in illite pores, providing a theoretical basis for the optimization and development of shale reservoirs.
Effects of Fish Oil with Heat Treatment on Obesity, Inflammation, and Gut Microbiota in Ovariectomized Mice
Authors: Fan Rong , Kim Judy , Kim Young-Cheul , Chung Soonkyu
Published: February 2025

Background/Objectives: Menopause induces substantial metabolic changes, including a reduction in metabolic rate and an elevated risk of developing metabolic diseases. Fish oil (FO) supplementation has been shown to ameliorate menopause-associated metabolic risks. Hyperthermia treatment (HT) has recently gained attention for its potential to improve metabolic and immune health. However, it remains to be determined whether HT can confer metabolic benefits comparable to those of FO supplementation or enhance the metabolic benefits of FO supplementation. This study aims to delineate the distinctive and collaborative effects of HT and FO supplementation in mitigating menopause-associated metabolic dysfunction. Methods: Female C57BL/6 ovariectomized (OVX) mice were randomly assigned to four groups (n = 12/group) to evaluate the individual and combined effects of FO supplementation (5% w/w) and HT treatment. For HT, whole-body heat exposure was conducted at 40–41 °C for 30 min, 5 days per week. After 12 weeks, animals were used to evaluate the changes in glucose and lipid metabolism, obesity outcome, and inflammatory markers. The gut microbiome analysis was conducted from cecal content by 16S rRNA sequencing. Acute inflammation was induced by lipopolysaccharide (LPS) injection to evaluate inflammatory responses. Results: HT alone distinctively reduced weight gain, lowered core body temperature, and attenuated insulin resistance comparable to FO supplement in OVX mice. The collaborative effect of FO and HT was not evident in metabolic parameters but more prominent in attenuating proinflammatory responses and microbiota modulation. Conclusions: Our findings suggest that the combined treatment of FO supplementation and HT may serve as an effective strategy to mitigate menopause-associated immune susceptibility and metabolic dysfunction. These benefits are likely mediated, at least in part, through the reduction in inflammation and modulation of the gut microbiota.
Antioxidant Intake and Ovarian Reserve in Women Attending a Fertility Center
Authors: Maldonado-Cárceles Ana B. , Souter Irene , Li Ming-Chieh , Mitsunami Makiko , Dimitriadis Irene , Ford Jennifer B. , Mínguez-Alarcón Lidia , Chavarro Jorge E.
Published: February 2025

Background/Objectives: The aim of this study was to investigate the association between antioxidant intake and antral follicle count (AFC), a marker of ovarian reserve, in women attending a fertility clinic. Methods: We conducted an observational study with 567 women undergoing infertility evaluation at the Massachusetts General Hospital Fertility Center, who were enrolled in the Environment and Reproductive Health (EARTH) study. Participants filled out the lifestyle and health questionnaires and a validated food frequency questionnaire (FFQ) for assessing habitual dietary intake and underwent a transvaginal ultrasound to measure AFC. Intake of nutrients with direct antioxidant capacity (vitamin A, C, and E and carotenoids) and intake of antioxidant food sources were estimated from the FFQ. Adjusted Poisson regression models were fitted to assess the relationships between antioxidants and AFC while adjusting for potential confounders. Non-linearity was assessed with restricted cubic splines. Results: The median (interquartile range) age and AFC of participants were 35.0 (32.0–38.0) years and 13 (9–18), respectively. Our findings revealed a non-linear association between lycopene intake and AFC. There was a positive linear association with the highest AFC among women consuming approximately 6000 mcg/day of lycopene (p for non-linearity = 0.003). An inverse association was observed between retinol intake, predominantly from dairy foods, and AFC among women aged under 35 years (p-trend < 0.001 and 0.01, respectively). Conclusions: Our findings suggest that lycopene intake might influence the ovarian reserve in fertility patients. The observed inverse association with retinol, if confirmed, may reflect biological mechanisms different from oxidative stress. The underlying mechanisms of these associations remain to be elucidated and warrant further investigation.
Nutritional Habits in Crohn’s Disease Onset and Management
Authors: Papadimitriou Konstantinos , Deligiannidou Georgia-Eirini , Voulgaridou Gavriela , Giaginis Constantinos , Papadopoulou Sousana K.
Published: February 2025

Crohn’s disease (CD)’s activation factors are still unclear. However, they are reported to involve an interaction between genetic susceptibility and unhealthy lifestyle factors like smoking, alcohol consumption, low physical activity, low BMI (<18.5 kg/m2), and probably unbalanced nutritional habits. Therefore, the aim of the present review is to demonstrate the possible effects of different nutritional habits, before the occurrence of the disease, as crucial factors for the inception of CD activation. The structure of the present narrative review was conducted following the instructions of the “Review Academy of Nutrition and Dietetics Checklist”. It is well established that the consumption of specific foods and drinks, such as spicy and fatty foods, raw vegetables and fruits, dairy products, carbonated beverages, and coffee or tea, can provoke the exacerbation of CD symptoms. On the other hand, Mediterranean-oriented diets seem to provide an inverse association with the incidence of CD. Moreover, patients seem to have the knowledge to select foods that contribute to the remission of their symptoms. However, it is not clearly reported whether the onset of CD activation is due to lifelong unbalanced nutritional habits and their subsequent effect on gut microbiota secretion, which seems to be the gold standard for CD’s investigation. Therefore, more future studies should record, examine, and compare the nutritional habits between patients with CD (immediately after the disease’s diagnosis) and healthy populations in a lifelong manner, in order to reveal the possible influence of foods on CD onset.
Health Effects and Therapeutic Potential of the Gut Microbe Akkermansia muciniphila
Authors: Aja Ezinne , Zeng Amber , Gray Weston , Connelley Kaden , Chaganti Anil , Jacobs Jonathan P.
Published: February 2025

Akkermansia muciniphila is a bacterium commonly found in the human gastrointestinal tract that has received considerable interest as a potential probiotic for the improvement of gut health and overall metabolic function. A. muciniphila is enriched in the mucus layer of the intestinal lining, where it degrades mucin and plays a significant role in gut barrier maintenance and immune regulation. A higher abundance of A. muciniphila has been observed in the gut of healthy individuals relative to those with metabolic disorders, and multiple metabolic benefits, including improved glucose management, reduced body fat, and reduced inflammation have been linked to A. muciniphila. Current research on A. muciniphila primarily relies on mouse models, with limited human interventional studies available. While these animal studies offer valuable insights into the potential roles of A. muciniphila in health and disease, further clinical investigations in humans are needed to fully understand its impact. Here, we explore the current scope of A. muciniphila research and its potential as a therapeutic agent to improve gut and metabolic health while also emphasizing the need to optimize techniques to further improve studies of this organism.
Preoperative Nutrition in Bariatric Surgery: A Narrative Review on Enhancing Surgical Success and Patient Outcomes
Authors: Simancas-Racines Daniel , Frias-Toral Evelyn , Campuzano-Donoso Martín , Ramos-Sarmiento Daniel , Zambrano-Villacres Raynier , Reytor-González Claudia , Schiavo Luigi
Published: February 2025

Bariatric surgery has become the preferred treatment for individuals with morbid obesity. Nutrition is key in optimizing surgical outcomes by reducing risks and enhancing recovery. Preoperative strategies, such as reducing body fat, decreasing liver size, and improving metabolic profiles, have been shown to facilitate safer surgical procedures with fewer complications. This narrative review aims to provide an analysis of the fundamental role of preoperative nutritional management in improving bariatric surgery outcomes, emphasizing the importance of addressing specific nutritional challenges to enhance surgical safety, recovery, and overall health. Preoperative nutritional interventions focus on correcting comorbidities and nutritional deficiencies, particularly hypovitaminosis and micronutrient imbalances, through a multidisciplinary approach involving nutritionists and other healthcare professionals. These interventions not only prepare patients for the physiological demands of surgery but also initiate a period of adaptation to new dietary habits, aiming to improve long-term compliance and mitigate risks such as postoperative weight regain and dumping syndrome. Adopting dietary changes, such as very low-calorie or ketogenic diets 6–12 weeks before surgery, enhances adherence to postoperative restrictions and overall surgical success. Future research should focus on developing comprehensive guidelines for preoperative nutritional care to improve patient outcomes globally.
Targeting Helicobacter pylori Through the “Muco-Microbiotic Layer” Lens: The Challenge of Probiotics and Microbiota Nanovesicles
Authors: Manna Olga Maria , Caruso Bavisotto Celeste , Gratie Melania Ionelia , Damiani Provvidenza , Bonaventura Giuseppe , Cappello Francesco , Tomasello Giovanni , D’Andrea Vito
Published: February 2025

The muco-microbiotic layer represents a critical biological frontier in gastroenterology, emphasizing the intricate interplay between the protective mucus, its resident microbiota, and extracellular vesicles. This review explores the functional morphology of the gastric mucosa, focusing on the gastric muco-microbiotic layer, its role as a protective barrier, and its dynamic interaction with some of the most insidious pathogens such as Helicobacter pylori (H. pylori). Highlighting the multifaceted mechanisms of H. pylori pathogenesis, we have delved into bacterial virulence factors, host immune responses, and the microbiota’s regulatory effects. Novel therapeutic strategies for H. pylori eradication, including traditional antibiotic therapies and emerging adjuvant treatments like probiotics and probiotic-derived extracellular vesicles, are critically examined. These findings underscore the potential of targeting nanovesicular interactions in the gastric mucosa, proposing a paradigm shift in the management of H. pylori infections to improve patient outcomes while mitigating antibiotic resistance.
Community-Dwelling Filipino Older Adults’ Experiences with Virtual Coach for Health-Enhancing Physical Activity (HEPA): A Phenomenology
Authors: Dino Michael Joseph S. , Dion Kenneth W. , Abadir Peter M. , Budhathoki Chakra , Balbin Patrick Tracy , Malacas Ma. Kristina G. , Hernandez Rommel P. , Nicolas Jacquelyn Joyce G. , Barcial-Espinosa Jhal , Himmelfarb Cheryl R. Dennison , Davidson Patricia M. , Thiamwong Ladda
Published: February 2025

Background: Physical inactivity among older adults persists as one of the global burdens. Older adults’ physical activity (PA) levels decline simultaneously with age, causing health problems and poor outcomes. Immersive interventions, such as mixed reality virtual coaches, are gaining the potential to provide innovative solutions to prevent physical inactivity among older adults. However, an in-depth understanding of older adults’ experience in using virtual-coach-driven physical exercise programs remains unexplored. Purpose: This study explored the experiences of community-dwelling older adults in using a virtual-coach-driven physical exercise program. Design: This study applied a phenomenological design using the qualitative approach to determine the lived experiences of community-dwelling older adults about the mother project, the “Move and Groove for Older Adults Program” (Hataw at Sigla para kay Lolo at Lola), a virtual-coach-driven physical exercise program using MR. A semi-structured interview guide was crafted consisting of three domains (acceptability, barriers, and facilitators), each with two open-ended questions. MAXQDA was used to thematically analyze the qualitative data. Results: Nine key informants shared their experiences with the virtual coach-driven physical exercise program using mixed reality. Four themes were identified describing their experiences with the program: (1) “Looking around”: Exploring MR technology, (2) “Looking after”: Engaging with MR exercise peers, (3) “Looking at”: Examining issues with MR technology devices, and (4) “Looking Ahead”: Envisioning the future of MR exercises. Conclusions: The findings of this study introduced new concepts and challenged old notions regarding older adults’ technological engagement. The virtual-coach-driven physical exercise program enabled the participants to explore the novel methodology, enhance peer relationships, examine potential issues, and envision a future of possibilities using the technology. These outcomes are pertinent for progressing studies, scholars, and technology developers in incorporating MR into gerontechnology for wellness and fullness of life in the aging population. The mother project of this study was retrospectively registered [ID NCT06136468].
Survival of Endodontically Treated Teeth with Direct and Indirect Posts: A 5-Year Retrospective Evaluation at an Australian University Dental Clinic
Authors: Chau Tu Man , Sharma Lavanya Ajay , Xiao Lan , Li Zhiyuan , Abuzar Menaka
Published: February 2025

Restoring endodontically treated teeth has always been a challenge in dentistry. Dental posts are used when further retention is required for the final restoration, indicating that the tooth is already structurally compromised. Varying survival rates have been reported by previous studies, and there does not seem to be consensus on what factors are favorable regarding the survival of teeth treated with dental posts. Background/Objectives: This study investigated the survival of teeth treated with direct and indirect posts placed by dentists and dental students at an Australian university over a five-year period. Methods: This retrospective study included 684 direct and indirect posts placed from January 2018 to December 2022 at Griffith University dental clinic. The survival of the teeth treated with dental posts was assessed using Kaplan–Meier analysis. The potential effect of patient, tooth and operator factors was assessed using Cox regression analysis. Results: The posts had a five-year survival rate of 94% and survival time of 46.16 months. No statistically significant difference in survival was noted between the different groups in this study. Conclusions: Teeth treated with dental posts have been shown to have good short-term survival; however, long-term survival could not be commented on. Within the limitations of this study, survival was not associated with the location of the tooth, type of tooth, operator expertise, post material, cement, restoration or opposing dentition.
Investigating the In Vivo Effects of Anti-Prion Protein Nanobodies on Prion Disease with AAV Vector
Authors: Zhang Jingjing , Wang Mengfei , Wang Dan , Zhang Xiangyi , Ma Yue , Pardon Els , Steyaert Jan , Abskharon Romany , Wang Fei , Ma Jiyan
Published: February 2025

Prion diseases are fatal neurodegenerative disorders affecting humans and animals, and the central pathogenic event is the conversion of normal prion protein (PrPC) into the pathogenic PrPSc isoform. Previous studies have identified nanobodies that specifically recognize PrPC and inhibit the PrPC to PrPSc conversion in vitro. In this study, we investigated the potential for in vivo expression of anti-PrPC nanobodies and evaluated their impact on prion disease. The coding sequences of three nanobodies were packaged into recombinant adeno-associated virus (rAAV) and were administered via intracerebroventricular (ICV) injection in newborn mice. We found that the expression of these nanobodies remained robust for over 180 days, with no observed detrimental effects. To assess their therapeutic potential, we performed ICV injections of nanobody-expressing rAAVs in newborn mice, followed by intracerebral prion inoculation at 5–6 weeks of age. One nanobody exhibited a small yet statistically significant therapeutic effect, extending survival time from 176 days to 184 days. Analyses of diseased brains revealed that the nanobodies did not alter the pathological changes. Our findings suggest that high levels of anti-PrPC nanobodies are necessary to delay disease progression. Further optimization of the nanobodies, AAV vectors, or delivery methods is essential to achieve a significant therapeutic effect.
Merkel Cell Polyomavirus Co-Infection in HIV/AIDS Individuals: Clinical Diagnosis, Consequences and Treatments
Authors: Zhou Xianfeng , Yin Chenxue , Lin Ziqi , Yan Zhangren , Wang Jiangang
Published: February 2025

Merkel cell polyomavirus (MCV) was named for its role as the causative agent of Merkel cell carcinoma (MCC), which is MCV positive in approximately 80% of cases. MCV is classified as a Group 2A carcinogen, which promotes carcinogenesis by integrating T-antigen into the cell genome. The prevalence of anti-MCV antibodies in the general population can be as high as 90%. MCV typically promotes cancer by integrating T-antigen genes into the host cell genome, and 80% of MCC cases are attributed to MCV activation. In immunocompetent individuals, MCV usually remains latent after infection. However, the incidence of MCC increases significantly in immunocompromised or immunodeficient patients, such as those who have undergone organ transplantation, have chronic lymphocytic leukemia, or are living with human immunodeficiency virus (HIV) infection. Acquired immunodeficiency is a particular feature of people living with HIV. Currently, research on HIV/AIDS patients with MCV infection, clinical outcomes, and treatments is quite limited. This paper reviews previous research and systematically examines the relationship between HIV/AIDS and MCV-associated diseases, with the aim of providing valuable information for the prevention, diagnosis, and treatment of MCV in vulnerable populations.