bluuebunny/crossref_metadata_2025 huggingface dataset:
A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening
Authors: Rezaei Meysam , Razavi Bazaz Sajad , Morshedi Rad Dorsa , Shimoni Olga , Jin Dayong , Rawlinson William , Ebrahimi Warkiani Majid
Published: October 2021

The COVID-19 pandemic has changed people’s lives and has brought society to a sudden standstill, with lockdowns and social distancing as the preferred preventative measures. To lift these measurements and reduce society’s burden, developing an easy-to-use, rapid, and portable system to detect SARS-CoV-2 is mandatory. To this end, we developed a portable and semi-automated device for SARS-CoV-2 detection based on reverse transcription loop-mediated isothermal amplification followed by a CRISPR/Cas12a reaction. The device contains a heater element mounted on a printed circuit board, a cooler fan, a proportional integral derivative controller to control the temperature, and designated areas for 0.2 mL Eppendorf® PCR tubes. Our system has a limit of detection of 35 copies of the virus per microliter, which is significant and has the capability of being used in crisis centers, mobile laboratories, remote locations, or airports to diagnose individuals infected with SARS-CoV-2. We believe the current methodology that we have implemented in this article is beneficial for the early screening of infectious diseases, in which fast screening with high accuracy is necessary.
Sub-Part-Per-Billion Level Sensing of Fentanyl Residues from Wastewater Using Portable Surface-Enhanced Raman Scattering Sensing
Authors: Zhang Boxin , Hou Xingwei , Zhen Cheng , Wang Alan X.
Published: October 2021

Detection of illicit drug residues from wastewater provides a new route toward community-level assessment of drug abuse that is critical to public health. However, traditional chemistry analytical tools such as high-performance liquid chromatography in tandem with mass spectrometry (HPLC-MS) cannot meet the large-scale testing requirement in terms of cost, promptness, and convenience of use. In this article, we demonstrated ultra-sensitive and portable surface-enhanced Raman scattering sensing (SERS) of fentanyl, a synthetic opioid, from sewage water and achieved quantitative analysis through principal component analysis and partial least-squares regression. The SERS substrates adopted in this application were synthesized by in situ growth of silver nanoparticles on diatomaceous earth films, which show ultra-high sensitivity down to 10 parts per trillion in artificially contaminated tap water in the lab using a commercial portable Raman spectrometer. Based on training data from artificially contaminated tap water, we predicted the fentanyl concentration in the sewage water from a wastewater treatment plant to be 0.8 parts per billion (ppb). As a comparison, the HPLC-MS confirmed the fentanyl concentration was below 1 ppb but failed to provide a specific value of the concentration since the concentration was too low. In addition, we further proved the validity of our SERS sensing technique by comparing SERS results from multiple sewage water treatment plants, and the results are consistent with the public health data from our local health authority. Such SERS sensing technique with ultra-high sensitivity down to sub-ppb level proved its feasibility for point-of-care detection of illicit drugs from sewage water, which is crucial to assess public health.
Recent Advances on IoT-Assisted Wearable Sensor Systems for Healthcare Monitoring
Authors: Mamdiwar Shwetank Dattatraya , R Akshith , Shakruwala Zainab , Chadha Utkarsh , Srinivasan Kathiravan , Chang Chuan-Yu
Published: October 2021

IoT has played an essential role in many industries over the last few decades. Recent advancements in the healthcare industry have made it possible to make healthcare accessible to more people and improve their overall health. The next step in healthcare is to integrate it with IoT-assisted wearable sensor systems seamlessly. This review rigorously discusses the various IoT architectures, different methods of data processing, transfer, and computing paradigms. It compiles various communication technologies and the devices commonly used in IoT-assisted wearable sensor systems and deals with its various applications in healthcare and their advantages to the world. A comparative analysis of all the wearable technology in healthcare is also discussed with tabulation of various research and technology. This review also analyses all the problems commonly faced in IoT-assisted wearable sensor systems and the specific issues that need to be tackled to optimize these systems in healthcare and describes the various future implementations that can be made to the architecture and the technology to improve the healthcare industry.
The Role of Mesostriatal Dopamine System and Corticostriatal Glutamatergic Transmission in Chronic Pain
Authors: Ziółkowska Barbara
Published: October 2021

There is increasing recognition of the involvement of the nigrostriatal and mesolimbic dopamine systems in the modulation of chronic pain. The first part of the present article reviews the evidence indicating that dopamine exerts analgesic effects during persistent pain by stimulating the D2 receptors in the dorsal striatum and nucleus accumbens (NAc). Thereby, dopamine inhibits striatal output via the D2 receptor-expressing medium spiny neurons (D2-MSN). Dopaminergic neurotransmission in the mesostriatal pathways is hampered in chronic pain states and this alteration maintains and exacerbates pain. The second part of this article focuses on the glutamatergic inputs from the medial prefrontal cortex to the NAc, their activity changes in chronic pain, and their role in pain modulation. Finally, interactions between dopaminergic and glutamatergic inputs to the D2-MSN are considered in the context of persistent pain. Studies using novel techniques indicate that pain is regulated oppositely by two independent dopaminergic circuits linking separate parts of the ventral tegmental area and of the NAc, which also interact with distinct regions of the medial prefrontal cortex.
Is Developmental Dyslexia Due to a Visual and Not a Phonological Impairment?
Authors: Werth Reinhard
Published: October 2021

It is a widely held belief that developmental dyslexia (DD) is a phonological disorder in which readers have difficulty associating graphemes with their corresponding phonemes. In contrast, the magnocellular theory of dyslexia assumes that DD is a visual disorder caused by dysfunctional magnocellular neural pathways. The review explores arguments for and against these theories. Recent results have shown that DD is caused by (1) a reduced ability to simultaneously recognize sequences of letters that make up words, (2) longer fixation times required to simultaneously recognize strings of letters, and (3) amplitudes of saccades that do not match the number of simultaneously recognized letters. It was shown that pseudowords that could not be recognized simultaneously were recognized almost without errors when the fixation time was extended. However, there is an individual maximum number of letters that each reader with DD can recognize simultaneously. Findings on the neurobiological basis of temporal summation have shown that a necessary prolongation of fixation times is due to impaired processing mechanisms of the visual system, presumably involving magnocells and parvocells. An area in the mid-fusiform gyrus also appears to play a significant role in the ability to simultaneously recognize words and pseudowords. The results also contradict the assumption that DD is due to a lack of eye movement control. The present research does not support the assumption that DD is caused by a phonological disorder but shows that DD is due to a visual processing dysfunction.
Evaluation of Intervention Effectiveness of Sensory Compensatory Training with Tactile Discrimination Feedback on Sensorimotor Dysfunction of the Hand after Stroke
Authors: Kitai Ken , Odagiri Masashi , Yamauchi Ryosuke , Kodama Takayuki
Published: October 2021

We investigated the intervention effect of training using a feedback-type tactile discrimination system on sensorimotor dysfunction of the hand after a stroke. A human male subject with sensorimotor dysfunction in his left hand after a stroke was asked to perform peg manipulation practice, a building block stacking task, and a material identification task for 10 min each for six weeks. During the activities, a tactile discrimination feedback system was used. The system is a device that detects the vibration information generated when touching an object with a hand and that feeds back the captured information in real time as vibration information. After the intervention, in addition to the reorganization of the sensorimotor areas, the deep sensation, sense of agency, numbness, amount of use, and quality of the left-hand movement improved. Our results suggest that training with the use of a feedback system could be a new form of rehabilitation for sensorimotor dysfunction of the hand.
Sleep, Function, Behaviour and Cognition in a Cohort of Children with Down Syndrome
Authors: Chawla Jasneek K. , Bernard Anne , Heussler Helen , Burgess Scott
Published: October 2021

Objective: To describe the sleep problems experienced by children with Down syndrome attending a tertiary sleep clinic and relationship with behaviour, function and cognition. Methods: Data were collected from children with Down syndrome aged 3–18 years old. Carers completed the Abbreviated Child Sleep Habits Questionnaire, Child Behaviour Checklist and Life-Habits Questionnaire at enrolment. Cognitive assessment (Stanford-Binet 5) was undertaken by a trained psychologist. Children received management for their sleep problem as clinically indicated. Results: Forty-two subjects with a median age of 6.8 years (Interquartile Range-IQR 4.5, 9.8) were enrolled. A total of 92% were referred with snoring or symptoms of Obstructive Sleep Apnoea (OSA), with 79% of those referred having had previous ENT surgery. Thus, 85% of all participants underwent a sleep study and 61% were diagnosed with OSA (OAHI ≥ 1/h). Based on questionnaires, 86% of respondents indicated that their child had a significant sleep disorder and non-respiratory sleep problems were common. Non-respiratory problems included: trouble going to sleep independently (45%), restless sleep (76%), night-time waking (24%) and bedtime resistance (22%). No significant correlations were found between sleep measures (behavioural and medical sleep problems) and the behavioural, functional or cognitive parameters. Conclusion: Sleep disorders were very common, especially non-respiratory sleep problems. OSA was common despite previous surgery. No association was found between sleep-related problems (snoring, sleep-study-confirmed OSA or non-respiratory sleep problem) and parent-reported behavioural problems, functional impairments or intellectual performance. This may reflect limitations of the measures used in this study, that in this population ongoing problems with daytime function are not sleep related or that a cross-sectional assessment does not adequately take into account the impacts of past disease/treatments. Further research is required to further evaluate the tools used to evaluate sleep disorders, the impact of those disorder on children with Down syndrome and interventions which improve both sleep and daytime function.
Analysis of Seismic Action on the Tie Rod System in Historic Buildings Using Finite Element Model Updating
Authors: Ereiz Suzana , Duvnjak Ivan , Damjanović Domagoj , Bartolac Marko
Published: October 2021

Historic buildings have a high architectural value and their maintenance, repair and rehabilitation require a special approach. This approach is mainly based on the buildings’ performance under non-destructive tests such as operational modal analysis (OMA). Under extreme loads, such as earthquakes, these buildings require representative numerical models to simulate their expected response. In historic buildings, tie rods transfer axial loads and are typically used to balance horizontal trust due to static and dynamic loads associated with seismic actions. It is very important to determine the possibility of exceeding their load-bearing capacity under extreme loads, such as an earthquake. In this context, this paper presents an approach for the analysis of seismic action on the tie rod system in a historic building. The analysis was performed by combining the on-site experimental testing and the finite element model updating (FEMU) of the local models of tie rods and the global model of the structure. It was shown that the combination of analyzing local and global structural models, experimental on-site testing and FEMU is a viable solution for assessment of historic buildings’ load bearing capacity.
Framing and Evaluating the Best Practices of IFC-Based Automated Rule Checking: A Case Study
Authors: Sobhkhiz Soroush , Zhou Yu-Cheng , Lin Jia-Rui , El-Diraby Tamer E.
Published: October 2021

This research reviews recent advances in the domain of Automated Rule Checking (ARC) and argues that current systems are predominantly designed to validate models in post-design stages, useful for applications such as e-permitting. However, such a design-check-separated paradigm imposes a burden on designers as they need to iteratively fix the fail-to-pass issues. Accordingly, the study reviews the best-practices of IFC-based ARC systems and proposes a framework for ARC system development, aiming to achieve proactive bottom-up solutions building upon the requirements and resources of end-users. To present and evaluate its capabilities, the framework is implemented in a real-life case study. The case study presents all the necessary steps that should be taken for the development of an ARC solution from rule selection and analysis, to implementation and feedback. It is explained how a rule checking problem can be broken down into separate modules implemented in an iterative approach. Results show that the proposed framework is feasible for successful implementation of ARC systems and highlight that a stable data standard and modeling guideline is needed to achieve proactive ARC solutions. The study also discusses that there are some critical limitations in using IFC which need to be addressed in future studies.
In-Situ and Predicted Performance of a Certified Industrial Passive House Building under Future Climate Scenarios
Authors: Conroy Alison , Mukhopadhyaya Phalguni , Wimmers Guido
Published: October 2021

The Wood Innovation Research Lab was designed as a low energy-use building to facilitate the construction and testing of engineered wood products by the faculty and staff of the Master of Engineering in Integrated Wood Design Program at the University of Northern British Columbia in Prince George, BC, Canada. Constructed using a 533 mm thick-wall and 659 mm flat roof assembly, it received certification as Canada’s first industrial facility built to the International Passive House standard. Temperature and humidity sensors were installed in the north and south exterior wall assemblies to measure long-term hygrothermal performance. Data collected between 2018–2020 shows no record of long-term moisture accumulation within the exterior assemblies. Data collected during this time period was used to validate hygrothermal performance models for the building created using the WUFI® Plus software. Long-term performance models created using future climate data for five cities across Canada under two global warming scenarios shows favorable results, with an increase in average annual temperatures resulting in lower average relative humidity values at the interior face of the exterior sheathing board in the exterior wall assemblies.
Melanoma Recognition by Fusing Convolutional Blocks and Dynamic Routing between Capsules
Authors: Pérez Eduardo , Ventura Sebastián
Published: October 2021

Skin cancer is one of the most common types of cancers in the world, with melanoma being the most lethal form. Automatic melanoma diagnosis from skin images has recently gained attention within the machine learning community, due to the complexity involved. In the past few years, convolutional neural network models have been commonly used to approach this issue. This type of model, however, presents disadvantages that sometimes hamper its application in real-world situations, e.g., the construction of transformation-invariant models and their inability to consider spatial hierarchies between entities within an image. Recently, Dynamic Routing between Capsules architecture (CapsNet) has been proposed to overcome such limitations. This work is aimed at proposing a new architecture which combines convolutional blocks with a customized CapsNet architecture, allowing for the extraction of richer abstract features. This architecture uses high-quality 299×299×3 skin lesion images, and a hyper-tuning of the main parameters is performed in order to ensure effective learning under limited training data. An extensive experimental study on eleven image datasets was conducted where the proposal significantly outperformed several state-of-the-art models. Finally, predictions made by the model were validated through the application of two modern model-agnostic interpretation tools.
Management of Adverse Events and Supportive Therapy in Relapsed/Refractory Multiple Myeloma
Authors: Pozzi Samantha , Bari Alessia , Pecherstorfer Martin , Vallet Sonia
Published: October 2021

Relapsed/refractory (RR) multiple myeloma (MM) patients are a fragile population because of prolonged drug exposure and advanced age. Preserving a good quality of life is of high priority for these patients and the treatment of disease- and treatment-related complications plays a key role in their management. By preventing and limiting MM-induced complications, supportive care improves patients’ outcome. Erythropoietin-stimulating agents and bisphosphonates are well-established supportive strategies, yet novel agents are under investigation, such as anabolic bone agents and activin receptor-like kinase (ALK) inhibitors. The recent dramatic changes in the treatment landscape of MM pose an additional challenge for the routine care of RRMM patients. Multidrug combinations in first and later lines increase the risk for long-lasting toxicities, including adverse cardiovascular and neurological events. Moreover, recently approved first-in-class drugs have unique side-effect profiles, such as ocular toxicity of belantamab mafodotin or gastrointestinal toxicity of selinexor. This review discusses current standards in supportive treatment of RRMM patients, including recommendations in light of the recent SARS-CoV-19 pandemic, and critically looks at the incidence and management of side effects of standard as well as next generation anti-MM agents.
Epigenetic DNA Modifications Upregulate SPRY2 in Human Colorectal Cancers
Authors: Stuckel Alexei J. , Zeng Shuai , Lyu Zhen , Zhang Wei , Zhang Xu , Dougherty Urszula , Mustafi Reba , Zhang Qiong , Joshi Trupti , Bissonnette Marc , Choudhury Samrat Roy , Khare Sharad
Published: October 2021

Conventional wisdom is that Sprouty2 (SPRY2), a suppressor of Receptor Tyrosine Kinase (RTK) signaling, functions as a tumor suppressor and is downregulated in many solid tumors. We reported, for the first time, that increased expression of SPRY2 augments cancer phenotype and Epithelial-Mesenchymal-Transition (EMT) in colorectal cancer (CRC). In this report, we assessed epigenetic DNA modifications that regulate SPRY2 expression in CRC. A total of 4 loci within SPRY2 were evaluated for 5mC using Combined Bisulfite Restriction Analysis (COBRA). Previously sequenced 5hmC nano-hmC seal data within SPRY2 promoter and gene body were evaluated in CRC. Combined bioinformatics analyses of SPRY2 CRC transcripts by RNA-seq/microarray and 450K methyl-array data archived in The Cancer Genome Atlas (TCGA) and GEO database were performed. SPRY2 protein in CRC tumors and cells was measured by Western blotting. Increased SPRY2 mRNA was observed across several CRC datasets and increased protein expression was observed among CRC patient samples. For the first time, SPRY2 hypomethylation was identified in adenocarcinomas in the promoter and gene body. We also revealed, for the first time, increases of 5hmC deposition in the promoter region of SPRY2 in CRC. SPRY2 promoter hypomethylation and increased 5hmC may play an influential role in upregulating SPRY2 in CRC.
7-Epitaxol Induces Apoptosis and Autophagy in Head and Neck Squamous Cell Carcinoma through Inhibition of the ERK Pathway
Authors: Kumar V. Bharath , Hsieh Ming-Ju , Mahalakshmi B. , Chuang Yi-Ching , Lin Chia-Chieh , Lo Yu-Sheng , Ho Hsin-Yu , Lin Jen-Tsun
Published: October 2021

As the main derivative of paclitaxel, 7-Epitaxol is known to a have higher stability and cytotoxicity. However, the anticancer effect of 7-Epitaxol is still unclear. The purpose of this study was to explore the anticancer effects of 7-Epitaxol in squamous cell carcinoma of the head and neck (HNSCC). Our study findings revealed that 7-Epitaxol potently suppressed cell viability in SCC-9 and SCC-47 cells by inducing cell cycle arrest. Flow cytometry and DAPI staining demonstrated that 7-Epitaxol treatment induced cell death, mitochondrial membrane potential and chromatin condensation in OSCC cell lines. The compound regulated the proteins of extrinsic and intrinsic pathways at the highest concentration, and also increased the activation of caspases 3, 8, 9, and PARP in OSCC cell lines. Interestingly, a 7-Epitaxol-mediated induction of LC3-I/II expression and suppression of p62 expression were observed in OSCC cells lines. Furthermore, the MAPK inhibitors indicated that 7-Epitaxol induces apoptosis and autophagy marker proteins (cleaved-PARP and LC3-I/II) by reducing the phosphorylation of ERK1/2. In conclusion, these findings indicate the involvement of 7-Epitaxol in inducing apoptosis and autophagy through ERK1/2 signaling pathway, which identify 7-Epitaxol as a potent cytotoxic agent in HNSCC.
Autophagy Is Polarized toward Cell Front during Migration and Spatially Perturbed by Oncogenic Ras
Authors: Singh Manish Kumar , Zago Giulia , Veith Irina , Camonis Jacques , Coppey Mathieu , Parrini Maria Carla
Published: October 2021

Autophagy is a physiological degradation process that removes unnecessary or dysfunctional components of cells. It is important for normal cellular homeostasis and as a response to a variety of stresses, such as nutrient deprivation. Defects in autophagy have been linked to numerous human diseases, including cancers. Cancer cells require autophagy to migrate and to invade. Here, we study the intracellular topology of this interplay between autophagy and cell migration by an interdisciplinary live imaging approach which combines micro-patterning techniques and an autophagy reporter (RFP-GFP-LC3) to monitor over time, during directed migration, the back–front spatial distribution of LC3-positive compartments (autophagosomes and autolysosomes). Moreover, by exploiting a genetically controlled cell model, we assessed the impact of transformation by the Ras oncogene, one of the most frequently mutated genes in human cancers, which is known to increase both cell motility and basal autophagy. Static cells displayed an isotropic distribution of autophagy LC3-positive compartments. Directed migration globally increased autophagy and polarized both autophagosomes and autolysosomes at the front of the nucleus of migrating cells. In Ras-transformed cells, the front polarization of LC3 compartments was much less organized, spatially and temporally, as compared to normal cells. This might be a consequence of altered lysosome positioning. In conclusion, this work reveals that autophagy organelles are polarized toward the cell front during migration and that their spatial-temporal dynamics are altered in motile cancer cells that express an oncogenic Ras protein.
CDK1-Mediated Phosphorylation of BAG3 Promotes Mitotic Cell Shape Remodeling and the Molecular Assembly of Mitotic p62 Bodies
Authors: Luthold Carole , Lambert Herman , Guilbert Solenn M. , Rodrigue Marc-Antoine , Fuchs Margit , Varlet Alice-Anaïs , Fradet-Turcotte Amélie , Lavoie Josée N.
Published: October 2021

The cochaperone BCL2-associated athanogene 3 (BAG3), in complex with the heat shock protein HSPB8, facilitates mitotic rounding, spindle orientation, and proper abscission of daughter cells. BAG3 and HSPB8 mitotic functions implicate the sequestosome p62/SQSTM1, suggesting a role for protein quality control. However, the interplay between this chaperone-assisted pathway and the mitotic machinery is not known. Here, we show that BAG3 phosphorylation at the conserved T285 is regulated by CDK1 and activates its function in mitotic cell shape remodeling. BAG3 phosphorylation exhibited a high dynamic at mitotic entry and both a non-phosphorylatable BAG3T285A and a phosphomimetic BAG3T285D protein were unable to correct the mitotic defects in BAG3-depleted HeLa cells. We also demonstrate that BAG3 phosphorylation, HSPB8, and CDK1 activity modulate the molecular assembly of p62/SQSTM1 into mitotic bodies containing K63 polyubiquitinated chains. These findings suggest the existence of a mitotically regulated spatial quality control mechanism for the fidelity of cell shape remodeling in highly dividing cells.
Advances in Colorimetric Assay Based on AuNPs Modified by Proteins and Nucleic Acid Aptamers
Authors: Melikishvili Sopio , Piovarci Ivan , Hianik Tibor
Published: October 2021

This review is focused on the biosensing assay based on AuNPs (AuNPs) modified by proteins, peptides and nucleic acid aptamers. The unique physical properties of AuNPs allow their modification by proteins, peptides or nucleic acid aptamers by chemisorption as well as other methods including physical adsorption and covalent immobilization using carbodiimide chemistry or based on strong binding of biotinylated receptors on neutravidin, streptavidin or avidin. The methods of AuNPs preparation, their chemical modification and application in several biosensing assays are presented with focus on application of nucleic acid aptamers for colorimetry assay for determination of antibiotics and bacteria in food samples.
Conductive Hydrogel-Based Electrochemical Sensor: A Soft Platform for Capturing Analyte
Authors: Fu Li , Yu Aimin , Lai Guosong
Published: October 2021

Electrode modifications for electrochemical sensors attract a lot of attention every year. Among them, hydrogels are a relatively special class of electrode modifier. Since hydrogels often contain polymers, even though they are conductive polymers, they are not ideal electrode modifiers because of their poor conductivity. However, the micro-aqueous environment and the three-dimensional structure of hydrogels are an excellent platform for immobilizing bioactive molecules and maintaining their activity. This gives the hydrogel-modified electrochemical sensor the potential to perform specific recognition. At the same time, the rapid development of nanomaterials also makes the composite hydrogel have good electrical conductivity. This has led many scientists to become interested in hydrogel-based electrochemical sensors. In this review, we summarize the development process of hydrogel-based electrochemical sensors, starting from 2000. Hydrogel-based electrochemical sensors were initially used only as a carrier for biomolecules, mostly for loading enzymes and for specific recognition. With the widespread use of noble metal nanoparticles and carbon materials, hydrogels can now be used to prepare enzyme-free sensors. Although there are some sporadic studies on the use of hydrogels for practical applications, the vast majority of reports are still limited to the detection of common model molecules, such as glucose and H2O2. In the review, we classify hydrogels according to their different conducting strategies, and present the current status of the application of different hydrogels in electrochemical sensors. We also summarize the advantages and shortcomings of hydrogel-based electrochemical sensors. In addition, future prospects regarding hydrogel for electrochemical sensor use have been provided at the end.
Does Oxygen Content Play a Role in Spontaneous Closure of Perimembranous Ventricular Septal Defects?
Authors: Bassareo Pier Paolo , Calcaterra Giuseppe , Deidda Martino , Marras Andrea Raffaele , Mercuro Giuseppe
Published: October 2021

(1) Background: the impact of a series of laboratory parameters (haemoglobin, haematocrit, foetal haemoglobin, peripheral oxygen saturation, iron, transferrin, ferritin, and albumin) on perimembranous ventricular septal defects spontaneous healing was tested. (2) Methods: one hundred and seven patients were enrolled in the study (57% males; mean age 2.1 ± 0.4 years) and were subsequently subdivided into two groups: self-healing (n = 36) and in need of intervention (n = 71). Self-healing subjects were defined on the basis of an absence of residual shunts at colorDoppler across the previous defect. (3) Results: no statistically significant differences were reported in the size of perimembranous ventricular septal defects between the two groups (p = ns). Conversely, prevalence of anaemia was significantly higher in those requiring intervention than in the self-healing group (p < 0.03), while haemoglobin, iron, ferritin, and albumin levels were lower (p < 0.001, p < 0.05, p < 0.02, p < 0.007, respectively). In multivariable linear regression analysis, only haemoglobin and albumin were found to be associated with spontaneous closure (p < 0.005 and p < 0.02, respectively). In multiple logistic regression analysis, haemoglobin independently increased the probability of self-healing of perimembranous ventricular septal defects (p = 0.03). All patients needing an interventional closure of perimembranous ventricular septal defects presented with haemoglobin <12.7 g/dL. (4) Conclusion: the self-resolution of perimembranous ventricular septal defects seems to rely on numerous factors, including oxygen content, which is likely to promote cell proliferation as well as tissue regeneration. Haemoglobin blood concentration seems to influence the natural history of perimembranous ventricular septal defects and improvement of anaemia by supplementation of iron intake might represent a simple and reliable method to promote self-healing.
The Intertemporal Role of Respiratory Support in Improving Neonatal Outcomes: A Narrative Review
Authors: Sarafidis Kosmas , Chotas William , Agakidou Eleni , Karagianni Paraskevi , Drossou Vasiliki
Published: October 2021

Defining improvements in healthcare can be challenging due to the need to assess multiple outcomes and measures. In neonates, although progress in respiratory support has been a key factor in improving survival, the same degree of improvement has not been documented in certain outcomes, such as bronchopulmonary dysplasia. By exploring the evolution of neonatal respiratory care over the last 60 years, this review highlights not only the scientific advances that occurred with the application of invasive mechanical ventilation but also the weakness of the existing knowledge. The contributing role of non-invasive ventilation and less-invasive surfactant administration methods as well as of certain pharmacological therapies is also discussed. Moreover, we analyze the cost–benefit of neonatal care-respiratory support and present future challenges and perspectives.
Effects of an Internet-Based and Teacher-Facilitated Sexuality Education Package: A Cluster-Randomized Trial
Authors: Jin Zhao , Guo Fuyu , Wang Kai , Zhang Hanxiyue , Cao Wenzhen , Hee Jiayi , Yuan Yuan , Chen Minne , Tang Kun
Published: October 2021

Background: This study aimed to evaluate the effects of an internet-based and teacher-facilitated sexuality education package on the sexual knowledge and attitudes of Chinese adolescents. Methods: Six middle schools where no sexuality education had been performed with a total of 501 adolescent students (245 males and 256 females) were included in the trial. In total, 14 classes were randomly assigned to the intervention (internet-based sexuality education package) or the control group (classes were conducted as per normal). Students’ sexual knowledge and attitudes were assessed at the baseline, at the end of the intervention, and 12 months after the intervention. Generalized linear models were employed to assess the effects of the intervention. Results: Positive effects of the intervention were observed on sexual knowledge (β = 4.65, 95% CI: 4.12—5.17) and attitudes (β = 1.25, 95% CI: 1.00—1.50) at the end of the intervention. After 12 months, the effects sustained but the magnitude declined for sexual knowledge (β = 2.39, 95% CI: 1.85—2.93) and attitudes (β = 0.49, 95% CI: 0.23—0.75). There were no significant differences between male and female students. Conclusions: Although further modifications are required, the sexuality education package can increase the accessibility of comprehensive sexuality education to adolescents in rural areas in China.
Prediction Model for Bronchopulmonary Dysplasia in Preterm Newborns
Authors: Jassem-Bobowicz Joanna Maria , Klasa-Mazurkiewicz Dagmara , Żawrocki Anton , Stefańska Katarzyna , Domżalska-Popadiuk Iwona , Kwiatkowski Sebastian , Preis Krzysztof
Published: October 2021

OBJECTIVE: To develop a multifactorial model that allows the prediction of bronchopulmonary dysplasia (BPD) in preterm newborns. MATERIALS AND METHODS: A single-center retrospective study of infants born below 32 + 0 weeks gestational age. We created a receiver operating characteristic curve to assess the multifactorial BPD risk and calculate the BPD risk accuracy using the area under the curve (AUC). BPD risk was categorized using a multifactorial predictive model based on the weight of the evidence. RESULTS: Of the 278 analyzed preterm newborns, 127 (46%) developed BPD. The significant risk factors for BPD in the multivariate analysis were gestational age, number of red blood cell concentrate transfusions, number of surfactant administrations, and hemodynamically significant patent ductus arteriosus. The combination of these factors determined the risk of developing BPD, with an AUC value of 0.932. A multifactorial predictive model based on these factors, weighted by their odds ratios, identified four categories of newborns with mean BPD risks of 9%, 59%, 82%, and 100%. CONCLUSION: A multifactorial model based on easily available clinical factors can predict BPD risk in preterm newborns and inform potential preventive measures.
Non-Conventional Agricultural Spaces and Climate Change: The Cases of Le Grenier boréal and Lufa Farms in Quebec, Canada
Authors: Doyon Mélanie , Klein Juan-Luis
Published: October 2021

The objective of this text is to present a reflection on the link between local initiatives to combat food insecurity and actions adapting to climate change. To this end, two case studies of ongoing experiments in the Canadian province of Quebec will be presented and compared. While these two cases are very different in terms of location, production and people involved, they share the objective of bringing fresh and healthy food, produced locally, to the population of their territory and of rethinking the relationship of the community to nature through food production. Despite their significant differences, each of these two cases features actions for responding to problems that have a common cause: an agro-industrial food system that, by decoupling the locations of production and consumption, in order to maximize the economic profitability of the capital invested, has compromised both the health of citizens and the ecological balance.
Polyphenol-Rich Purified Bioactive Fraction Isolated from Terminalia catappa L.: UHPLC-MS/MS-Based Metabolite Identification and Evaluation of Their Antimicrobial Potential
Authors: Sowmya Tumakuru Nataraj , Raveesha Koteshwar Anandrao
Published: October 2021

Background: Antimicrobial resistance is a major threat to humankind and the advancement of resistance due to genetic modifications and other defense mechanisms that make the current antibiotics ineffective or less efficacious. Objective: This investigation aims to isolate bioactive compounds from the leaf acetone extract of Terminalia catappa and to evaluate their antimicrobial potential against human pathogenic organisms. Materials and Methods: The bioactive extract was subjected to column chromatography. The fractions were assessed for their minimum inhibitory concentration, minimum fungicidal concentration, and time kill assays. UHPLC-MS/MS analysis was used to identify the bioactive molecules in the fraction. Results: The isolated fraction exhibited antimicrobial activity, with the most sensitive being Staphylococcus aureus (clinical isolate) and Methicillin Resistant Staphylococcus aureus 1503 (0.097 mg/mL), and the fungi Trichophyton rubrum and Candida albicans were inhibited at 0.097 mg/mL. The time kill assay exhibited bactericidal properties towards S. aureus (clinical isolate) and Salmonella typhi (MTCC 733). Additionally, MRSA 1503 and Proteus vulgaris exhibited bacteriostatic activities. The UHPLC-MS/MS analysis revealed that the fraction was rich in polyphenols. Alkaloids and some ellagitannins were identified for the first time. Conclusion: The results highlight the significant inhibition of multidrug-resistant MRSA strains and fungi by the polyphenol-rich fraction. The investigation reveals the potential use of the identified compounds for antimicrobial use, which could lower the implication of multidrug resistance.
Optical Filter with Large Angular Dependence of Transmittance Using Liquid Crystal Devices
Authors: Takatoh Kohki , Ito Masahiro , Saito Suguru , Takagi Yuuta
Published: October 2021

This study proposed a new type of optical device with variable transmittance based on the incident angle direction. These devices consist of two liquid crystal devices (LCDs) with a half-wave plate between them. Hybrid aligned nematic (HAN)-type guest-host (GH) LCDs or GH-LCDs with antiparallel alignment of high pretilt angles were used. The use of a half-wave plate allowed for the control of the p- and s-waves. Using these devices, a wide range of transmittances were obtained because no polarizer was used. The newly proposed LCDs have a wide range of applications, including use on buildings, vehicles, and glasses.
Remote Gait Type Classification System Using Markerless 2D Video
Authors: Albuquerque Pedro , Machado João Pedro , Verlekar Tanmay Tulsidas , Correia Paulo Lobato , Soares Luís Ducla
Published: October 2021

Several pathologies can alter the way people walk, i.e., their gait. Gait analysis can be used to detect such alterations and, therefore, help diagnose certain pathologies or assess people’s health and recovery. Simple vision-based systems have a considerable potential in this area, as they allow the capture of gait in unconstrained environments, such as at home or in a clinic, while the required computations can be done remotely. State-of-the-art vision-based systems for gait analysis use deep learning strategies, thus requiring a large amount of data for training. However, to the best of our knowledge, the largest publicly available pathological gait dataset contains only 10 subjects, simulating five types of gait. This paper presents a new dataset, GAIT-IT, captured from 21 subjects simulating five types of gait, at two severity levels. The dataset is recorded in a professional studio, making the sequences free of background camouflage, variations in illumination and other visual artifacts. The dataset is used to train a novel automatic gait analysis system. Compared to the state-of-the-art, the proposed system achieves a drastic reduction in the number of trainable parameters, memory requirements and execution times, while the classification accuracy is on par with the state-of-the-art. Recognizing the importance of remote healthcare, the proposed automatic gait analysis system is integrated with a prototype web application. This prototype is presently hosted in a private network, and after further tests and development it will allow people to upload a video of them walking and execute a web service that classifies their gait. The web application has a user-friendly interface usable by healthcare professionals or by laypersons. The application also makes an association between the identified type of gait and potential gait pathologies that exhibit the identified characteristics.
Carcinoma with Triphasic Differentiation Arising from Inverted Papilloma in Sinonasal Sinus: A Rare Case with Molecular Characterization
Authors: Kim So-Woon , Na Kiyong
Published: October 2021

Small cell neuroendocrine carcinoma (SNEC) is a rare subset of tumors in the sinonasal sinus. Combined tumors are exceedingly rare. Here, we describe a 65-year-old male with a mixed tumor of SNEC and sarcomatoid carcinoma arising in an inverted papilloma, containing squamous cell carcinoma in situ (SqCCis) in the sinonasal sinus. We evaluated the molecular characteristics of the two separate carcinoma components using next-generation sequencing. The patient presented with a nasal obstruction. Computed tomography showed a mass infiltrating the right ethmoid and maxillary sinuses. An excisional biopsy was performed. The tumor was found to have three morphologically distinct components. The first was SqCCis arising in an inverted papilloma, which was positive for cytokeratin and P40. The second consisted of nests of densely packed small round cells representing SNEC-positive neuroendocrine markers. The third was a solid sheet of anaplastic spindle cell proliferation, which was negative for the above markers. Oncogenic mutations such as FBXW7, TP53, and EGFR were detected in both SNEC and sarcomatoid carcinoma, and MYCL amplification was observed only in the SNEC component. This case highlights an extremely rare presentation of combined SNEC and sarcomatoid carcinoma arising from an inverted papilloma in the sinonasal sinus.
Identification of piRNA Targets in Urinary Extracellular Vesicles for the Diagnosis of Prostate Cancer
Authors: Peng Qiang , Chiu Peter Ka-Fung , Wong Christine Yim-Ping , Cheng Carol Ka-Lo , Teoh Jeremy Yuen-Chun , Ng Chi-Fai
Published: October 2021

Emerging studies demonstrate that PIWI-interacting RNAs (piRNAs) are associated with various human cancers. This study aimed to evaluate the urinary extracellular vesicles (EVs) piRNAs as non-invasive biomarkers for prostate cancer (PCa) diagnosis. RNA was extracted from urinary EVs from five PCa patients and five healthy controls (HC), and the piRNAs were analyzed by small RNA sequencing. Dysregulated piRNAs were identified and then validated in another 30 PCa patients and 10 HC by reverse-transcription polymerase chain reaction (RT-qPCR). The expressions of novel_pir349843, novel_pir382289, novel_pir158533, and hsa_piR_002468 in urinary EVs were significantly increased in the PCa group compared with the HC group. The area under the curve (AUC) of novel_pir158533, novel_pir349843, novel_pir382289, hsa_piR_002468, and the combination of the four piRNA in PCa diagnosis was 0.723, 0.757, 0.777, 0.783, and 0.853, respectively. After the RNAhybrid program analysis, all four piRNAs had multiple potential binding sites with key mRNAs in PTEN/PI3K/Akt, Wnt/beta-catenin, or androgen receptor pathway, which are critical in PCa development and progression. In conclusion, our findings indicate that specific piRNAs in urinary EVs may serve as non-invasive diagnostic biomarkers for PCa.
Eagle’s Syndrome as a Cause of Discomfort and the Subjective Presence of a Foreign Body in the Throat
Authors: Wolińska Irena , Jaźwiec Przemysław , Pawłowska Maria , Gać Paweł , Poręba Rafał , Poręba Małgorzata
Published: October 2021

Eagle syndrome consists of symptoms resulting from the elongation and excessive calcification of the styloid process of the temporal bone and calcification of the ligaments associated with this process. The main symptoms of this syndrome are the feeling of a foreign body in the throat, dysphagia and pain localized in the temporomandibular region, neck and ear. The authors describe the case report of a previously healthy 39-year-old Caucasian male that complained of discomfort and foreign body sensation in his throat. Computed tomography (CT) showed the presence of an elongated styloid process bilaterally with clear predomination at the left side. The patient underwent laryngological and surgical consultation. Due to the lack of symptoms related to the compression of the carotid arteries, no surgery was recommended. In summary, Eagle’s syndrome is a rare condition characterized by craniofacial pain or foreign body sensation that should be considered, especially if the pain is unilateral. CT imaging in his case was a perfect tool and enabled a suitable diagnosis of this rare syndrome.
Sonoanatomy and Stepwise/Systematic Ultrasound Examination of the Extrinsic/Intrinsic Wrist Ligaments
Authors: Wang Jia-Chi , Wu Wei-Ting , Chang Ke-Vin , Chen Lan-Rong , Nakashima Yuko , Özçakar Levent
Published: October 2021

Ultrasound has emerged as the most useful imaging tool for investigating wrist and hand disorders, with several published ultrasound protocols having demonstrated their practicality in scanning the wrist tendons and nerves. However, ligaments of the wrist are networked in a complex manner, deterring sonographers from examining them with an organized strategy. Furthermore, because of the non-parallel alignment between the radiocarpal, mid-carpal, and carpal–metacarpal joints, precise recognition of the carpal bones is challenging, although ultrasound is paramount for visualizing the wrist ligaments. In this regard, the current article for point of view aims to elaborate sonoanatomy of the carpal bones and to present a stepwise systematic approach for navigating the extrinsic and intrinsic wrist ligaments.
Using Data Compression to Build a Method for Statistically Verified Attribution of Literary Texts
Authors: Ryabko Boris , Savina Nadezhda
Published: October 2021

We consider the problems of the authorship of literary texts in the framework of the quantitative study of literature. This article proposes a methodology for authorship attribution of literary texts based on the use of data compressors. Unlike other methods, the suggested one gives a possibility to make statistically verified results. This method is used to solve two problems of attribution in Russian literature.
Changes in Mathematics Core Curriculum and Matriculation Exam in the Light of the COVID-19-Shock
Authors: Csapodi Csaba , Hoffmann Miklós
Published: October 2021

The new National Core Curriculum came into force in September 2020 in Hungarian schools. The COVID-19 pandemic has had a deep impact on the final stages of its development. In this paper we have selected two areas for analysis: the fundamental principles of mathematics curriculum and the matriculation exam in mathematics. We propose improvements in both fields, further emphasizing the importance of skills in displaying, understanding and processing information, including visual information obtained as a source or outcome of a problem. We argue that representation, interpretation, and critical evaluation of data and information must be essential parts of the mathematics curriculum. In this context, we also propose a new type of task for the matriculation exam: a complex essay task. The ultimate goal is the development of cross-cutting competencies to support students to become citizens who can make responsible decisions based on the data and knowledge available.