bluuebunny/crossref_metadata_2025 huggingface dataset:
Peronosporales Species Associated with Strawberry Crown Rot in the Czech Republic
Authors: Pánek Matěj , Maňasová Marie , Wenzlová Jana , Zouhar Miloslav , Mazáková Jana
Published: March 2022

The symptoms of crown rot on strawberry plants are considered typical for the pathogen Phytophthora cactorum, which causes high losses of this crop. However, an unknown number of related species of pathogens of Peronosporales cause symptoms quite similar to those caused by P. cactorum. To determine their spectrum and importance, strawberry plants were sampled from 41 farms in the Czech Republic. The cultures were isolated from the symptomatic plants using the baiting method, with subsequent cultivation on a semiselective medium. Isolates were identified to the species level using nuclear ribosomal internal transcribed spacer (ITS) barcoding after preliminary morphological determination. In total, 175 isolates of 24 species of Phytophthora, Phytopythium, Pythium, and Globisporangium were detected. The most represented was Phytophthora cactorum, with 113 (65%) isolates, which was recorded in 61% of farms, and the Pythium dissotocum complex with 20 (11%) isolates, which was recorded in 27% of farms. Other species were represented in units of percent. Large differences between farms in the species spectra were ascertained. The differences between species in cardinal growth temperatures and different management of the farms are discussed as a main reason for such a diversification. Regarding the dissimilar sensitivity of various species of Peronosporales against fungicides, the proper determination of the cause of disease is of crucial significance in plant protection.
Becoming More-than-Human: Realizing Earthly Eudaimonia to (E)coflourish through an Entangled Ethos
Authors: Quartz Sean
Published: March 2022

Organisms across the biosphere are experiencing extinction rates so dire that scientists have marked the Anthropocene as the sixth mass extinction in the planet’s history. Accordingly, plants and animals, by and large, are not flourishing on this deathly planet. Yet, perhaps it is possible for these more-than-humans to thrive––to realize eudaimonia, an ancient Greek concept meaning to flourish by living well––when humans reimagine their relationships with the natural world. In this study, I augment critical animal and media studies with creative cultural studies to arrive at creative/critical animal and media studies. Through this framework, I utilize rhetorical criticism to analyze how the documentary My Octopus Teacher reimagines interspecies relations to offer alternative pathways for earthly eudaimonia, a life approach centered on (e)coflourishing. I find the octopus, through its entangled ethos, teaches the human sensitized compassion with a significant result: the more-than-human octopus transfers her animality to the human who evolves to become more-than-human as well. I offer two arguments: first, contemplating earthly eudaimonia through an entangled ethos creates a space for ecological reflection; this space invites audiences to approach the more-than-human world with sensitized compassion and animality; second, analyzing the documentary through a creative/critical animal and media studies lens offers a unique perspective that foregrounds exploring imaginaries for peaceful, earthly coexistence while maintaining a critical focus against speciesism.
Prospective Evaluation of Ghrelin and Des-Acyl Ghrelin Plasma Levels in Children with Newly Diagnosed Epilepsy: Evidence for Reduced Ghrelin-to-Des-Acyl Ghrelin Ratio in Generalized Epilepsies
Authors: Costa Anna-Maria , Lo Barco Tommaso , Spezia Elisabetta , Conti Valerio , Roli Laura , Marini Lorenza , Minghetti Sara , Caramaschi Elisa , Pietrangelo Laura , Pecoraro Luca , D’Achille Fabio , Accorsi Paola , Trenti Tommaso , Melani Federico , Marini Carla , Guerrini Renzo , Darra Francesca , Bergonzini Patrizia , Biagini Giuseppe
Published: March 2022

Children with epilepsy and identified as responders to antiseizure medications (ASMs) were found to present markedly higher ghrelin plasma levels when compared to drug-resistant patients. However, it was undetermined if this phenotype could be influenced by the ASMs. Here, we prospectively investigated total ghrelin and des-acyl ghrelin (DAG) plasma levels by enzyme-linked immunosorbent assay before and after ASM administration. Inclusion criteria were: (i) subject with a suspicion of epilepsy; (ii) age ranging from 0 to 16 years; and (iii) informed consent signed by parents or caregivers. Exclusion criteria were acute or chronic metabolic disorders with occasional convulsions but without epilepsy. Fifty patients were followed over a period of one year in Italian neuropediatric centers. Apart from a few exceptions, the majority of children were responsive to ASMs. No differences were found in total ghrelin and DAG levels before and after the treatment, but total ghrelin levels were significantly lower in children with generalized epilepsy compared to those with combined focal and generalized epilepsy. Moreover, the ghrelin-to-DAG ratio was also markedly lower in generalized epilepsies compared to all the other types of epilepsy. Finally, ghrelin was unchanged by ASMs, including the first (e.g., carbamazepine), second (levetiracetam), and third (lacosamide) generation of anticonvulsants.
Old Sacred Trees as Memories of the Cultural Landscapes of Southern Benin (West Africa)
Authors: Atindehou Massogblé M. Lucrèce , Avakoudjo Hospice G. Gracias , Idohou Rodrigue , Azihou Fortuné Akomian , Assogbadjo Achille Ephrem , Adomou Aristide Cossi , Sinsin Brice
Published: March 2022

Large old trees (LOTs) are emblematic elements of the cultural landscape and can live for hundreds of years. They represent an intermediate aspect of cultural heritage, linking spirits and humans. They can also provide a range of ecosystem services. In spite of their importance, declining numbers have been reported. This study examined the diversity of LOTs and the impact of anthropogenic threats on their preservation in three districts of southern Benin: Ketou and Abomey, which represent historical districts with royal courts, and Lokossa, which does not have a tradition as a dynastic seat. Semi-structured interviews focused on ethnobotanical aspects and storytelling were conducted with a total of 150 community leaders and traditional practitioners; these were coupled with an inventory of LOTs to demonstrate their importance in maintaining the heritage and providing ecosystem services in cultural landscapes. Diversity, cultural importance, and ethnobotanical indices were calculated to compare positive and negative attitudes towards LOTs by the local people of the study areas. A total of 270 LOT individuals belonging to 14 species were recorded. The most common species was Adansonia digitata (70 individuals), followed by Milicia excelsa (47 individuals), Ceiba pentandra (37 individuals), and Blighia sapida (25 individuals). Sacred forests and the royal palaces (ten for Abomey and three for Kétou), which are protected by a traditional veto, had the highest number of LOTs (145 individuals) belonging to nine species. Details of 79 specific uses were documented for each plant part of LOTs. The most frequently reported were related to medicinal (80.64%), cultic (16.65%), and craft uses (2.6%). Based on a standard area of 100 km2, mean Shannon diversity (H’) and evenness (J) were lower in the cultural landscape of Ketou (H′ = 0.26 ± 0.42; J = 0.23 ± 0.37) compared to Lokossa (H′ = 0.27 ± 0.32; J = 0.21 ± 0.24) and Abomey (H′ = 0.42 ± 0.37; J = 0.35 ± 0.31). The threat patterns suggest that, irrespective of the species involved, certain determining factors (urbanization (35%), the timber trade (18%), and decisions made during the Marxist–Leninist revolution in Benin in 1972 (11%)) have affected and continue to affect LOT abundance and diversity. For better management of LOTs, there is a need to promote decision-making strategies that better align human cultural values and ecological objectives.
Obligations of Researchers and Managers to Respect Wetlands: Practical Solutions to Minimizing Field Monitoring Impacts
Authors: Bryzek Jessica A. , Noe Krista L. , De Silva Sindupa , MacKenzie Andrew , Von Haugg Cindy L. , Hartman Donna , McCall Jordan E. , Veselka Walter , Anderson James T.
Published: March 2022

Research and field monitoring can disturb wetland integrity. Adoption of ethical field practices is needed to limit monitoring induced stressors such as trampling, non-native seed and invertebrate dispersal, and disease and fungal spread. We identify a linear pathway of deterioration highlighting stressors that can progress to cumulative impacts, consequences, and losses at the site scale. The first step to minimize disturbance is to assess and classify the current ecosystem quality. We present a tiered framework for wetland classification and link preventative measures to the wetland tier. Preventative measures are recommended at various intensities respective to the wetland tier, with higher tiered wetlands requiring more intense preventative measures. In addition, preventative measures vary by time of implementation (before, during, and after the wetland visit) to mitigate impacts at various temporal scales. The framework is designed to increase transparency of field monitoring impacts and to promote the adoption of preventative measures. Implementing preventative measures can build accountability and foster a greater appreciation for our roles as researchers and managers in protecting wetlands.
Cochlear Implantation in Patients with Mitochondrial Gene Mutation: Decline in Speech Perception in Retrospective Long-Term Follow-Up Study
Authors: Kanemoto Kai , Kashio Akinori , Ogata Erika , Akamatsu Yusuke , Koyama Hajime , Uranaka Tsukasa , Hoshi Yujiro , Iwasaki Shinichi , Yamasoba Tatsuya
Published: March 2022

Clinical evidence of the effectiveness of cochlear implantation for hearing loss with mitochondrial DNA mutation is limited. Most reports have only described short-term postoperative speech perception, which may not reflect the limitations of cochlear implantation caused by progressive retrocochlear dysfunction. The present study aimed to investigate long-term speech perception after cochlear implantation in patients with severe to profound hearing loss associated with mitochondrial DNA mutation. A retrospective chart review was performed on patients with mitochondrial DNA mutation who had undergone cochlear implantation at the Department of Otolaryngology and Head and Neck Surgery at the University of Tokyo Hospital. We extracted data on causative mutations, clinical types, clinical course, perioperative complications, and short-term and long-term postoperative speech perception. Nine patients with mitochondrial DNA mutation underwent cochlear implantation. The mean observation period was 5.5 ± 4.2 years (range, 1–13 years), and seven patients were followed for more than 3 years. Two of the seven patients who initially showed good speech perception exhibited deterioration during long-term follow-up. The absence of an acute progression of cognitive decline in patients, showing a gradual decrease in speech perception, suggests that the deterioration of speech perception was caused by progressive retrocochlear degeneration. Although most patients with mitochondrial DNA mutation maintained good speech perception for more than 3 years after cochlear implantation, retrocochlear degeneration could cause the deterioration of speech perception during long-term follow-up.
Wear Study of Cubic Boron Nitride (cBN) Cutting Tool for Machining of Compacted Graphite Iron (CGI) with Different Metalworking Fluids
Authors: Zhu Long , Evans Robert , Zhou Yan , Ren Fei
Published: March 2022

Due to its desirable mechanical properties, compacted graphite iron (CGI) has been used to replace conventional gray cast iron (CI) in various applications, such as automotive engine blocks and cylinder heads. However, the poor machinability of CGI can lead to excessive tool wear and consequently high manufacturing costs. Various strategies have been developed to improve the machinability of CGI, including optimizing machining parameters and the development of novel metalworking fluids. In this study, machining of CGI was conducted using cubic boron nitride (cBN) tools under different cutting speeds, with both soluble and full-synthetic water-based metalworking fluids at different levels of sulfur addition and water dilution. The effects of the metalworking fluids on the tool wear behavior were examined. Results showed that at 200 m/min cutting speed, the soluble metalworking fluid at 4% dilution and 0.3% sulfur compound exhibited the best performance, with a cutting distance reaching 23.8 km. In contrast, the least effective soluble metalworking fluid at 9% dilution and 0.3% sulfur compound resulted in a 28.6% decrease in the cutting distance (17.0 km) compared to the best one. At a higher speed (300 m/min), the cutting distance for all metalworking fluids dropped to less than 6.0 km, with the full-synthetic metalworking fluid showing the shortest cutting distance of 4.8 km.
4′-(N-(Propargyl)pyrrol-2-yl)-2,2′:6′,2′′-terpyridine
Authors: Husson Jérôme , Guyard Laurent
Published: March 2022

A new terpyridine molecule, bearing a N-propargylated pyrrole, was prepared and characterized. Its synthesis was based on a Krohnke-type reaction between 2-acetylpyridine and N-propargylpyrrole-2-carboxaldehyde in a basic medium. An allene-containing terpyridine was also obtained as a by-product.
Spiral Thermal Plumes in Water under Conventional Heating: Numerical Results on the Effect of Rotation
Authors: Navarro María Cruz , Castaño Damián , Herrero Henar
Published: March 2022

In this paper, we study numerically the effect of rotation within a sample of water in a cylindrical container subject to rotation which is heated with a constant temperature at the bottom and lateral wall. We analyze the temporal behavior of temperature and flow velocity of the solvent. The thermal plumes developed at lower levels, already observed in the case without rotation, begin to spiral spreading outwards by the effect of rotation, increasing the azimuthal velocity of the fluid. No significant increases in the radial and vertical velocity components are observed which do not favor the mixture of hotter and colder flows in the sample and a faster heating of the solvent. In the rotation range studied, the state loses the axisymmetry and becomes fully 3D earlier in time as the rotation rate increases. To perform simulations, we use a 3D temporal model that couples momentum and heat equations and is based on spectral element methods.
An Online Semi-Definite Programming with a Generalized Log-Determinant Regularizer and Its Applications
Authors: Liu Yaxiong , Moridomi Ken-ichiro , Hatano Kohei , Takimoto Eiji
Published: March 2022

We consider a variant of the online semi-definite programming problem (OSDP). Specifically, in our problem, the setting of the decision space is a set of positive semi-definite matrices constrained by two norms in parallel: the L∞ norm to the diagonal entries and the Γ-trace norm, which is a generalized trace norm with a positive definite matrix Γ. Our setting recovers the original one when Γ is an identity matrix. To solve this problem, we design a follow-the-regularized-leader algorithm with a Γ-dependent regularizer, which also generalizes the log-determinant function. Next, we focus on online binary matrix completion (OBMC) with side information and online similarity prediction with side information. By reducing to the OSDP framework and applying our proposed algorithm, we remove the logarithmic factors in the previous mistake bound of the above two problems. In particular, for OBMC, our bound is optimal. Furthermore, our result implies a better offline generalization bound for the algorithm, which is similar to those of SVMs with the best kernel, if the side information is involved in advance.
Double Diffusive Natural Convection in a Square Cavity Filled with a Porous Media and a Power Law Fluid Separated by a Wavy Interface
Authors: Kolsi Lioua , Hussain Shafqat , Ghachem Kaouther , Jamal Muhammad , Maatki Chemseddine
Published: March 2022

This study deals with the influence of a wavy interface separating two layers filled with power law fluid and porous media, respectively. The governing equations are solved using the Finite Element Method (FEM) and the numerical model is validated by comparing with experimental findings. The parameters governing the studied configuration are varied as: Rayleigh number (103 ≤ Ra ≤ 106), power law index (0.6 ≤ n ≤ 1.4), Darcy number (10−2 ≤ Da ≤ 10−6), buoyancy ratio (0.1 ≤ N ≤ 10) and Lewis number (1 ≤ Le ≤ 10). It is inferred that the temperature gradient increases by augmenting the Rayleigh number, as the flow is observed from the vertical to horizontal direction in both layers. Constant enhancement in the heat and mass transfer is also observed by enriching the buoyancy effect. Moreover, the average Nusselt and Sherwood numbers decline by increasing the width of the porous layer.
Adaptive Nonsingular Terminal Sliding Mode Control for Performance Improvement of Perturbed Nonlinear Systems
Authors: Alattas Khalid A. , Vu Mai The , Mofid Omid , El-Sousy Fayez F. M. , Alanazi Abdullah K. , Awrejcewicz Jan , Mobayen Saleh
Published: March 2022

In this study, an adaptive nonsingular terminal sliding mode control technique according to the barrier function is designed for the performance improvement and robust stability of nonlinear systems with outdoor disturbances. For this reason, a novel nonlinear sliding surface is presented based on the states of the system. The nonlinear sliding surface forces the states of the system to converge from initial conditions to zero. Subsequently, a non-singular terminal sliding control scheme is advised for the purpose of finite-time stability of the nonlinear switching surface. Finite-time stabilization of the non-singular terminal sliding surface is verified by the Lyapunov theory. For improvement of the system performance against exterior perturbation, the barrier function adaptive technique is employed to estimate the unknown upper bounds of the exterior disturbance. Finally, the advantage and productivity of the recommended control method is investigated based on the simulation results. In the simulation part, the plasma torch jerk chaotic system is considered as a case study, such that the obtained results are given in different scenarios.
A Dimension Group-Based Comprehensive Elite Learning Swarm Optimizer for Large-Scale Optimization
Authors: Yang Qiang , Zhang Kai-Xuan , Gao Xu-Dong , Xu Dong-Dong , Lu Zhen-Yu , Jeon Sang-Woon , Zhang Jun
Published: March 2022

High-dimensional optimization problems are more and more common in the era of big data and the Internet of things (IoT), which seriously challenge the optimization performance of existing optimizers. To solve these kinds of problems effectively, this paper devises a dimension group-based comprehensive elite learning swarm optimizer (DGCELSO) by integrating valuable evolutionary information in different elite particles in the swarm to guide the updating of inferior ones. Specifically, the swarm is first separated into two exclusive sets, namely the elite set (ES) containing the top best individuals, and the non-elite set (NES), consisting of the remaining individuals. Then, the dimensions of each particle in NES are randomly divided into several groups with equal sizes. Subsequently, each dimension group of each non-elite particle is guided by two different elites randomly selected from ES. In this way, each non-elite particle in NES is comprehensively guided by multiple elite particles in ES. Therefore, not only could high diversity be maintained, but fast convergence is also likely guaranteed. To alleviate the sensitivity of DGCELSO to the associated parameters, we further devise dynamic adjustment strategies to change the parameter settings during the evolution. With the above mechanisms, DGCELSO is expected to explore and exploit the solution space properly to find the optimum solutions for optimization problems. Extensive experiments conducted on two commonly used large-scale benchmark problem sets demonstrate that DGCELSO achieves highly competitive or even much better performance than several state-of-the-art large-scale optimizers.
Pain Intensity and Degree of Disability after Fragility Fractures of the Pelvis
Authors: Filip Alexandru , Veliceasa Bogdan , Puha Bogdan , Filip Nina , Cojocaru Elena , Pertea Mihaela , Carp Claudiu Adrian , Huzum Bogdan , Alexa Ovidiu , Rommens Pol Maria
Published: March 2022

Background and objectives: Fragility fractures of the pelvis (FFP) are of increasing interest lately, being associated with a loss of mobility and affecting the quality of life. The aim of our study was to investigate the effect of FFP on disability and pain in patients, after one year since injury. Materials and Methods: In the study, we included 76 patients diagnosed with FFP, who were admitted to our trauma department between January 2016 and January 2019, and were above 65 years of age. The Von Korff pain intensity and disability scores were calculated in the hospital at 6 months and after 1 year. Results: Fifty-four patients were female (71%), with an average age of 75.9 ± 7.19 years. Twenty-two patients were male (29%) and had a mean age of 77.22 ± 7.33 years. We did not record significant differences regarding age between the men and women (p > 0.05). Significant improvements appeared between the baseline and the 6 month follow-up; the average pain intensity score at 6 months was 44.94 (SD 21.20) (p < 0.001), and the disability score was 54.30 (SD 21.62). The following average pain intensity and disability scores after 12 months were similar to the values at6 months: 44.48 (SD 21.74) for pain intensity and 52.36 (SD 24.53) for disability. The Von Korff pain score at 6 months and after 1 year depends on gender and on the initial Von Korff pain score (p = 0.02). The Von Korff disability score at 6 months depends on gender, the baseline pain score and the baseline disability score (p = 0.001). Conclusions: our patients reported long-lasting pain that had a severe effect on their daily routines, and they could not return to their normal status prior to injury.
Paraneoplastic Wong-Type Dermatomyositis Associated with Gynecological Malignancy
Authors: Russo Roberto , Gasparini Giulia , Cozzani Emanuele , Gravina Brunella , Parodi Aurora
Published: March 2022

Although dermatomyositis is known to be a possible paraneoplastic syndrome, often in the setting of gynecological cancers, Wong-type dermatomyositis—a rare variant of dermatomyositis—has not been clearly associated with internal malignancies to date. There is only one report from Japan of a woman who developed Wong-type dermatomyositis together with the recurrence of uterine cancer. We report the case of a Caucasian patient who presented with infrequent Wong-type dermatomyositis with positive anti-TIF1γ antibodies; screening for internal malignancies revealed fallopian tube carcinoma.
pH and Design on n–Alkyl Alcohol Bulk Liquid Membranes for Improving Phenol Derivative Transport and Separation
Authors: Albu Paul Constantin , Tanczos Szidonia-Katalin , Ferencz (Dinu) Andreea , Pîrțac Andreia , Grosu Alexandra Raluca , Pașcu Dumitru , Grosu Vlad-Alexandru , Bungău Constantin , Nechifor Aurelia Cristina
Published: March 2022

Regardless of the type of liquid membrane (LM) (Bulk Liquid Membranes (BLM), Supported Liquid Membranes (SLM) or Emulsion Liquid Membranes (ELM)), transport and separation of chemical species are conditioned by the operational (OP) and constructive design parameters (DP) of the permeation module. In the present study, the pH of the aqueous source phase (SP) and receiving phase (RP) of the proposed membrane system were selected as operational parameters. The mode of contacting the phases was chosen as the convective transport generator. The experiments used BLM-type membranes with spheres in free rotation as film contact elements of the aqueous phases with the membrane. The target chemical species were selected in the range of phenol derivatives (PD), 4–nitrophenol (NP), 2,4–dichlorophenol (DCP) and 2,4–dinitrophenol (DNP), all being substances of technical-economic and environmental interest. Due to their acid character, they allow the evaluation of the influence of pH as a determining operational parameter of transport and separation through a membrane consisting of n–octanol or n–decanol (n–AlcM). The comparative study performed for the transport of 4–nitrophenol (NP) showed that the module based on spheres (Ms) was more performant than the one with phase dispersion under the form of droplets (Md). The sphere material influenced the transport of 4–nitrophenol (NP). The transport module with glass spheres (Gl) was superior to the one using copper spheres (Cu), but especially with the one with steel spheres (St). In all the studied cases, the sphere-based module (Ms) had superior transport results compared to the module with droplets (Md). The extraction efficiency (EE) and the transport of 2,4–dichlorophenol (DCP) and 2,4–dinitrophenol (DNP), studied in the module with glass spheres, showed that the two phenolic derivatives could be separated by adjusting the pH of the source phase. At the acidic pH of the source phase (pH = 2), the two derivatives were extracted with good results (EE > 90%), while for pH values ranging from 4 to 6, they could be separated, with DCP having doubled separation efficiency compared to DNP. At a pH of 8 in the source phase, the extraction efficiency halved for both phenolic compounds.
Effect of Cr and Al on Elastic Constants of FeCrAl Alloys Investigated by Molecular Dynamics Method
Authors: Dai Hui , Yu Miaosen , Dong Yibin , Setyawan Wahyu , Gao Ning , Wang Xuelin
Published: March 2022

The FeCrAl alloy system is recognized as one of the candidate materials for accident-tolerant fuel (ATF) cladding in the nuclear power industry due to its high oxidation resistance under irradiation and high-temperature environments. The concentrations of Cr and Al have a significant effect on elastic properties of the FeCrAl alloy. In this work, elastic constants C11, C12, C44, bulk modulus and shear modulus of FeCrAl alloy were calculated with molecular dynamics methods. We explored compositions with 1–15 wt.% Cr and 1–5 wt.% Al at temperatures from 0 K to 750 K. The results show that the concentrations of Al and Cr have different effects on the elastic constants. When the concentration of Al was fixed, a decrease in bulk modulus and shear modulus with increasing Cr content was observed, consistent with previous experimental results. The dependence of elastic constants on temperature was also the same as in the experiments. Investigations into elastic properties of defect-containing alloys have shown that vacancies, voids, interstitials and Cr-rich precipitations have different effects on elastic properties of FeCrAl alloys. Investigations of elastic properties of defect-containing alloys have shown that vacancies, void, interstitials and Cr-rich precipitations have different effects on elastic properties of FeCrAl alloys. Therefore, the present results indicate that both the Cr and Al concentrations and radiation defects should be considered to develop and apply the FeCrAl alloy in ATF design.
Friction Stir Welding Prospective on Light-Alloys Joints
Authors: Cabibbo Marcello
Published: March 2022

The demand for stronger, lighter, and cost-effective materials for engineering applications is a key technological challenge [...]
Effect of Nano-Sized TiC-TiB2 on Microstructure and Properties of Twin-Roll Cast Al-Cu-Mn-Zr Alloy
Authors: Cao Jiaming , Wang Bao , Liu Xiao , Chang Ting , Zhao Qinglong
Published: March 2022

Al-5Cu-0.8Mn-0.1Zr strips produced during a twin-roll-casting process are investigated in this paper. Central segregation does occur in the alloy strip, and the segregation is a mixture of Al2Cu and Al20Cu2Mn3 eutectic phases. This mixture is difficult to be dissolved in solid solution heat treatment. In this paper, nano-sized TiC/TiB2 particles are introduced in the process of twin-roll-casting. It was found that after adding 0.3% nano-sized TiC-TiB2 particles, the composition distribution becomes more uniform, and the central segregation is eliminated, compared to the strip without nanoparticles. The tensile strength of T6-treated alloy increases from 350 MPa to 390 MPa when nanoparticles are added, and the goal of increasing the properties of twin-roll-cast Al-Cu-Mn-Zr alloy has been achieved.
Influence of Heat Input on Microstructure and Mechanical Properties of Gas Tungsten Arc Welded HSLA S500MC Steel Joints
Authors: Kornokar Kianoosh , Nematzadeh Fardin , Mostaan Hossein , Sadeghian Amirhossein , Moradi Mahmoud , Waugh David G. , Bodaghi Mahdi
Published: March 2022

High-strength low alloy (HSLA) S500MC steel is widely used for chassis components, structural parts, and pressure vessels. In this study, the effects of heat input during automatic gas tungsten arc welding (GTAW) on microstructure and mechanical properties of thermomechanically controlled processed (TMP) S500MC steel were investigated. A butt joint configuration was used, and welding was performed in autogenous mode. Six different levels of heat input namely 1.764 kJ/mm, 1.995 kJ/mm, 2.035 kJ/mm, 2.132 kJ/mm, 2.229 kJ/mm, and 2.33 kJ/mm were considered. Microstructural investigations revealed a different microstructure than base metal in the fusion zone (FZ) of all welded joints which was most likely due to a lath martensitic microstructure surrounded by retained austenite. With increased heat input, the amount of retained austenite and the size of packets increased. In the heat-affected zone (HAZ), two distinct regions of coarse grain (CG-HAZ) and fine grain (FG-HAZ) were observed. Due to the presence of carbides in the HAZ, mostly a martensitic microstructure with smaller packets, compared to FZ, was formed. By increasing the heat input and through the dissolution of carbides, the dimension of packets increased. Due to microstructural changes and grain growth, in both the FZ and the HAZ, the mechanical properties produced by TMP were lost in these regions. However, failure occurred in the base metal of all samples with a maximum tensile strength of 690 MPa. Thus, tensile strength in the weld zone and HAZ were higher than the base metal even for the highest heat input indicating the formation of a good joint between S500MC plates with GTAW, regardless of heat input.
Salivary Metabolomics for Oral Squamous Cell Carcinoma Diagnosis: A Systematic Review
Authors: Nijakowski Kacper , Gruszczyński Dawid , Kopała Dariusz , Surdacka Anna
Published: March 2022

Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer in which the consumption of tobacco and alcohol is considered to be the main aetiological factor. Salivary metabolome profiling could identify novel biochemical pathways involved in the pathogenesis of various diseases. This systematic review was designed to answer the question “Are salivary metabolites reliable for the diagnosis of oral squamous cell carcinoma?”. Following the inclusion and exclusion criteria, nineteen studies were included (according to PRISMA statement guidelines). In all included studies, the diagnostic material was unstimulated whole saliva, whose metabolome changes were determined by different spectroscopic methods. At the metabolic level, OSCC patients differed significantly not only from healthy subjects but also from patients with oral leukoplakia, lichen planus or other oral potentially malignant disorders. Among the detected salivary metabolites, there were the indicators of the impaired metabolic pathways, such as choline metabolism, amino acid pathways, polyamine metabolism, urea cycle, creatine metabolism, glycolysis or glycerolipid metabolism. In conclusion, saliva contains many potential metabolites, which can be used reliably to early diagnose and monitor staging in patients with OSCC. However, further investigations are necessary to confirm these findings and to identify new salivary metabolic biomarkers.
Optimization of Feedback FET with Asymmetric Source Drain Doping Profile
Authors: Lee Inyoung , Park Hyojin , Nguyen Quan The , Kim Garam , Cho Seongjae , Cho Ilhwan
Published: March 2022

A feedback field-effect transistor (FBFET) is a novel device that uses a positive feedback mechanism. FBFET has a high on-/off ratio and is expected to realize ideal switching characteristics through steep changes from off-state to on-state. In this paper, we propose and optimize FBFET devices with asymmetric source/drain doping concentrations. Additionally, we discuss the changes in electrical characteristics across various channel length and channel thickness conditions and compare them with those of FBFET with a symmetric source/drain. This shows that FBFET with an asymmetric source/drain has a higher on-/off ratio than FBFET with a symmetric source/drain.
Polymer/Silica Hybrid Waveguide Thermo-Optic VOA Covering O-Band
Authors: Yin Yuexin , Yao Mengke , Ding Yingzhi , Xu Xinru , Li Yue , Wu Yuanda , Zhang Daming
Published: March 2022

In this paper, a polymer/silica hybrid waveguide thermo-optic variable optical attenuator (VOA), covering the O-band, is demonstrated. The switch is fabricated by simple and low-cost direct ultraviolet (UV) lithography. The multimode interferences (MMIs) used in the Mach–Zehnder interferometer (MZI)-VOA are well optimized to realize low loss and large bandwidth. The VOA shows an extinction ratio (ER) of 18.64 dB at 1310 nm, with a power consumption of 8.72 mW. The attenuation is larger than 6.99 dB over the O-band. The rise and fall time of the VOA are 184 μs and 180 μs, respectively.
Design and Performance Analysis of a Micro-Displacement Worktable Based on Flexure Hinges
Authors: Yan Lan , Jiang Anna , Jiang Feng , Liu Guangda , Wang Fuzeng , Wu Xian
Published: March 2022

The flexure hinge is a kind of micro-displacement adjustment device with application prospects because of its high displacement resolution, positioning accuracy and repeatability. In this study, a micro-displacement worktable with four degrees of freedom (X→, Z→, X︵, Z︵) was designed. The micro-displacement worktable was composed of three different flexure hinges. The adjustment ranges and adjustment accuracy of flexure hinges in terms of their respective degrees were improved. The micro-displacement worktable performance was examined by FEA (Finite Element Method). The maximum displacement that was adjusted in X→ and Z→ was 1.67 µm and 1.74 µm. The maximum angle adjusted in the X︵ and Z︵ direction was 14.90° and 18.58°. A test platform was developed for micro-displacement worktable performance tests. The simulation results showed a good agreement with the experimental results.
Fabrication and Characterization of the Micro-Heater and Temperature Sensor for PolyMUMPs-Based MEMS Gas Sensor
Authors: Algamili Abdullah S. , Khir Mohd Haris , Ahmed Abdelaziz Y. , Rabih Almur A. , Ba-Hashwan Saeed S. , Alabsi Sami S. , Al-Mahdi Osamah L. , Isyaku Usman B. , Ahmed Mawahib G. , Junaid Muhammad
Published: March 2022

This work describes the fabrication and characterization of a Micro-Electro-Mechanical System (MEMS) sensor for gas sensing applications. The sensor is based on standard PolyMUMPs (Polysilicon Multi-Users MEMS Process) technology to control the temperature over the sensing layer. Due to its compact size and low power consumption, micro-structures enable a well-designed gas-sensing-layer interaction, resulting in higher sensitivity compared to the ordinary materials. The aim of conducting the characterization is to compare the measured and calculated resistance values of the micro-heater and the temperature sensor. The temperature coefficient of resistance (TCR) of the temperature sensor has been estimated by raising and dropping the temperature throughout a 25–110 °C range. The sensitivity of these sensors is dependent on the TCR value. The temperature sensor resistance was observed to rise alongside the rising environmental temperatures or increasing voltages given to the micro-heater, with a correlation value of 0.99. When compared to the TCR reported in the literature for the gold material 0.0034 °C−1, the average TCR was determined to be 0.00325 °C−1 and 0.0035 °C−1, respectively, indicating inaccuracies of 4.6% and 2.9%, respectively. The variation between observed and reported values is assumed to be caused by the fabrication tolerances of the design dimensions or material characteristics.
The Tip of Brucella O-Polysaccharide Is a Potent Epitope in Response to Brucellosis Infection and Enables Short Synthetic Antigens to Be Superior Diagnostic Reagents
Authors: Duncombe Lucy , Howells Laurence , Haughey Anna , Taylor Andrew V. , Kaveh Daryan , Erdenliğ Gϋrbilek Sevil , Dell Anne , Hitchen Paul G. , Haslam Stuart M. , Mandal Satadru Sekhar , Ganesh N. Vijaya , Bundle David R. , McGiven John
Published: March 2022

Brucellosis is a global disease and the world’s most prevalent zoonosis. All cases in livestock and most cases in humans are caused by members of the genus Brucella that possess a surface O-polysaccharide (OPS) comprised of a rare monosaccharide 4-deoxy-4-formamido-D-mannopyranose assembled with α1,2 and α1,3 linkages. The OPS of the bacterium is the basis for serodiagnostic tests for brucellosis. Bacteria that also contain the same rare monosaccharide can induce antibodies that cross-react in serological tests. In previous work we established that synthetic oligosaccharides, representing elements of the Brucella A and M polysaccharide structures, were excellent antigens to explore the antibody response in the context of infection, immunisation and cross reaction. These studies suggested the existence of antibodies that are specific to the tip of the Brucella OPS. Sera from naturally and experimentally Brucella abortus-infected cattle as well as from cattle experimentally infected with the cross-reactive bacterium Yersinia enterocolitica O:9 and field sera that cross react in conventional serological assays were studied here with an expanded panel of synthetic antigens. The addition of chemical features to synthetic antigens that block antibody binding to the tip of the OPS dramatically reduced their polyclonal antibody binding capability providing conclusive evidence that the OPS tip (non-reducing end) is a potent epitope. Selected short oligosaccharides, including those that were exclusively α1,2 linked, also demonstrated superior specificity when evaluated with cross reactive sera compared to native smooth lipopolysaccharide (sLPS) antigen and capped native OPS. This surprising discovery suggests that the OPS tip epitope, even though common to both Brucella and Y. enterocolitica O:9, has more specific diagnostic properties than the linear portion of the native antigens. This finding opens the way to the development of improved serological tests for brucellosis.
Absence of Streptococcus pneumoniae Capsule Increases Bacterial Binding, Persistence, and Inflammation in Corneal Infection
Authors: Carr Mary A. , Marcelo Dennis , Lovell K. Michael , Benton Angela H. , Tullos Nathan A. , Norcross Erin W. , Myers Brandon , Robbins Marcus K. , Craddieth Hayley , Marquart Mary E.
Published: March 2022

The role of the pneumococcal polysaccharide capsule is largely unclear for Streptococcus pneumoniae keratitis, an ocular inflammatory disease that develops as a result of bacterial infection of the cornea. In this study, capsule-deficient strains were compared to isogenic parent strains in their ability to adhere to human corneal epithelial cells. One isogenic pair was further used in topical ocular infection of mice to assess the contribution of the capsule to keratitis. The results showed that non-encapsulated pneumococci were significantly more adherent to cells, persisted in significantly higher numbers on mouse corneas in vivo, and caused significant increases in murine ocular IL9, IL10, IL12-p70, MIG, and MIP-1-gamma compared to encapsulated S. pneumoniae. These findings indicate that the bacterial capsule impedes virulence and the absence of capsule impacts inflammation following corneal infection.
Efficient and Quality-Optimized Metagenomic Pipeline Designed for Taxonomic Classification in Routine Microbiological Clinical Tests
Authors: Buffet-Bataillon Sylvie , Rizk Guillaume , Cattoir Vincent , Sassi Mohamed , Thibault Vincent , Del Giudice Jennifer , Gangneux Jean-Pierre
Published: March 2022

Metagenomics analysis is now routinely used for clinical diagnosis in several diseases, and we need confidence in interpreting metagenomics analysis of microbiota. Particularly from the side of clinical microbiology, we consider that it would be a major milestone to further advance microbiota studies with an innovative and significant approach consisting of processing steps and quality assessment for interpreting metagenomics data used for diagnosis. Here, we propose a methodology for taxon identification and abundance assessment of shotgun sequencing data of microbes that are well fitted for clinical setup. Processing steps of quality controls have been developed in order (i) to avoid low-quality reads and sequences, (ii) to optimize abundance thresholds and profiles, (iii) to combine classifiers and reference databases for best classification of species and abundance profiles for both prokaryotic and eukaryotic sequences, and (iv) to introduce external positive control. We find that the best strategy is to use a pipeline composed of a combination of different but complementary classifiers such as Kraken2/Bracken and Kaiju. Such improved quality assessment will have a major impact on the robustness of biological and clinical conclusions drawn from metagenomic studies.
Detection of Zoonotic Gastrointestinal Pathogens in Dairy Sheep and Goats by Using FilmArray® Multiplex-PCR Technology
Authors: Tsilipounidaki Katerina , Florou Zoe , Lianou Daphne T. , Michael Charalambia K. , Katsarou Eleni I. , Skoulakis Anargyros , Fthenakis George C. , Petinaki Efthymia
Published: March 2022

The objectives of this study were (a) to detect gastrointestinal pathogens in faecal samples of sheep and goats using the FilmArray® GI Panel and (b) to evaluate factors that were associated with their presence. Faecal samples from ewes or does in 70 sheep flocks and 24 goat herds in Greece were tested for the presence of 22 gastrointestinal pathogens by means of the BioFire® FilmArray® Gastrointestinal (GI) Panel. The most frequently detected pathogens were Shiga-like toxin-producing Escherichia coli stx1/stx2 (94.7% of farms), Giardia lamblia (59.6%), and Campylobacter spp. (50.0% of farms). Other pathogens detected were Cryptosporidium spp., Salmonella spp., enterotoxigenic E. coli lt/st, Yersinia enterocolitica, E. coli O157, Rotavirus A, Shigella/enteroinvasive E. coli, and Plesiomonasshigelloides. There was a difference in the prevalence of detection of pathogens between sheep and goat farms only for Salmonella spp.: 18.3% versus 0.0%, respectively. Mixed infections were detected in 76 farms (80.9% of farms), specifically 57 sheep flocks and 19 goat herds, with on average, 2.5 ± 0.1 pathogens detected per farm. The body condition score of ewes in farms, in which only one pathogen was detected in faecal samples, was significantly higher than that of ewes in farms, in which at least two pathogens were detected: 2.55 ± 0.11 versus 2.31 ± 0.04. In sheep flocks, the number of pathogens in faecal samples was significantly higher in farms with semi-extensive management. In goat herds, the number of pathogens in faecal samples was positively correlated with average precipitation and inversely correlated with temperature range in the respective locations.
Contrasting Styles of Inter-Caldera Volcanism in a Peralkaline System: Case Studies from Pantelleria (Sicily Channel, Italy)
Authors: Romano Pierangelo , White John , Rotolo Silvio , Jordan Nina , Cirrincione Rosolino , De Giorgio Giovanni , Fiannacca Patrizia , Vaccaro Epifanio
Published: March 2022

The recent (<190 ka) volcanic history of Pantelleria is characterized by the eruption of nine peralkaline ignimbrites, ranging in composition from comenditic trachyte to comendite to pantellerite. The ~46 ka Green Tuff (GT) was the last of these ignimbrites, which was followed by many effusive and explosive low-volume eruptions of pantellerite from vents within the caldera moat and along the caldera rim. Although recent studies have shed additional light on the age, petrochemistry, and volcanology of the older ignimbrites, there is very little knowledge of magmatism that occurred between these older ignimbrites, primarily due to the very scarce exposures. In this paper, we present new field descriptions and geochemical data for three local peralkaline centers never studied before, two pre-GT and one post-GT, which share a similar setting with respect to the caldera scarps but differ in terms of their age, composition, and eruptive style. These centers include: (i) the older (~125 ka) Giache center (comenditic trachyte), (ii) the ~67 ka Attalora center (comendite, pantellerite), and (iii) the younger (~14 ka) Patite center (pantellerite).
Electrospun Nanofiber Membranes for Air Filtration: A Review
Authors: Zhou Yangjian , Liu Yanan , Zhang Mingxin , Feng Zhangbin , Yu Deng-Guang , Wang Ke
Published: March 2022

Nanomaterials for air filtration have been studied by researchers for decades. Owing to the advantages of high porosity, small pore size, and good connectivity, nanofiber membranes prepared by electrospinning technology have been considered as an outstanding air-filter candidate. To satisfy the requirements of material functionalization, electrospinning can provide a simple and efficient one-step process to fabricate the complex structures of functional nanofibers such as core–sheath structures, Janus structures, and other multilayered structures. Additionally, as a nanoparticle carrier, electrospun nanofibers can easily achieve antibacterial properties, flame-retardant properties, and the adsorption properties of volatile gases, etc. These simple and effective approaches have benefited from the significate development of electrospun nanofibers for air-filtration applications. In this review, the research progress on electrospun nanofibers as air filters in recent years is summarized. The fabrication methods, filtration performances, advantages, and disadvantages of single-polymer nanofibers, multipolymer composite nanofibers, and nanoparticle-doped hybrid nanofibers are investigated. Finally, the basic principles of air filtration are concluded upon and prospects for the application of complex-structured nanofibers in the field of air filtration are proposed.
Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology
Authors: Kamanina Olga A. , Saverina Evgeniya A. , Rybochkin Pavel V. , Arlyapov Vyacheslav A. , Vereshchagin Anatoly N. , Ananikov Valentine P.
Published: March 2022

Microorganism-cell-based biohybrid materials have attracted considerable attention over the last several decades. They are applied in a broad spectrum of areas, such as nanotechnologies, environmental biotechnology, biomedicine, synthetic chemistry, and bioelectronics. Sol-gel technology allows us to obtain a wide range of high-purity materials from nanopowders to thin-film coatings with high efficiency and low cost, which makes it one of the preferred techniques for creating organic-inorganic matrices for biocomponent immobilization. This review focuses on the synthesis and application of hybrid sol-gel materials obtained by encapsulation of microorganism cells in an inorganic matrix based on silicon, aluminum, and transition metals. The type of immobilized cells, precursors used, types of nanomaterials obtained, and their practical applications were analyzed in detail. In addition, techniques for increasing the microorganism effective time of functioning and the possibility of using sol-gel hybrid materials in catalysis are discussed.