bluuebunny/crossref_metadata_2025 huggingface dataset:
Relating the bivalve shellfish harvesting area classification criteria in the United States and European Union programmes
Authors: Lee R. J. , Reese R. A.
Published: June 2014

Estimation of the level of risk of faecal contamination of shellfish harvesting areas is undertaken by monitoring faecal indicator bacteria in seawater samples under the United States programme and shellfish flesh samples under the European Union (EU) programme. Determining the relationship between the two approaches is important for assessing the relative level of public health protection and regulating international trade. The relationship was investigated using both statistical modelling and simple compliance assessment on large international data sets of paired seawater and shellfish samples. The two approaches yielded the same conclusions: EU class A is more stringent than the US Approved category for all species; the US Restrictive standard is more restrictive than EU class B for some bivalve species. Therefore, the classifications under the two programmes are not exactly equivalent.
Proteomic regulation during Legionella pneumophila biofilm development: decrease of virulence factors and enhancement of response to oxidative stress
Authors: Khemiri Arbia , Lecheheb Sandra Ahmed , Chi Song Philippe Chan , Jouenne Thierry , Cosette Pascal
Published: June 2014

Legionella pneumophila (L. pneumophila) is a Gram-negative bacterium, which can be found worldwide in aquatic environments. It tends to persist because it is often protected within biofilms or amoebae. L. pneumophila biofilms have a major impact on water systems, making the understanding of the bacterial physiological adaptation in biofilms a fundamental step towards their eradication. In this study, we report for the first time the influence of the biofilm mode of growth on the proteome of L. pneumophila. We compared the protein patterns of microorganisms grown as suspensions, cultured as colonies on agar plates or recovered with biofilms formed on stainless steel coupons. Statistical analyses of the protein expression data set confirmed the biofilm phenotype specificity which had been previously observed. It also identified dozens of proteins whose abundance was modified in biofilms. Proteins corresponding to virulence factors (macrophage infectivity potentiator protein, secreted proteases) were largely repressed in adherent cells. In contrast, a peptidoglycan-associated lipoprotein (Lpg2043) and a peroxynitrite reductase (Lpg2965) were accumulated by biofilm cells. Remarkably, hypothetical proteins, that appear to be unique to the Legionella genus (Lpg0563, Lpg1111 and Lpg1809), were over-expressed by sessile bacteria.
Supercritical Fluid Extraction and Stabilization of Phenolic Compounds
From Natural Sources – Review (Supercritical Extraction and Stabilization
of Phenolic Compounds)
Authors: Junior Mário Roberto Maróstica , Leite Alice Vieira , Dragano Nathalia Romanelli Vicente
Published: June 2014

Phenolic compounds are components commonly found in fruits and vegetables. Recently, they have been subject
of considerable scientific and therapeutic interest mainly due to their antioxidant properties and related healthpromoting
benefits, as confirmed by the numerous papers devoted to various properties of these compounds. Thus, the extractions
of phenolic compounds with functional properties from natural sources have attracted special interests. Supercritical
fluid extraction offers a number of advantages over conventional extraction methods above all in relation to the
conditions employed during the extraction process that ensures minimal alteration of the active phenolics and the healing
properties could be more easily preserved. This work provides an updated overview on the principal applications of Supercritical
fluid extraction in recovery of bioactive phenolics emphasizing the effects of temperature, pressure, addition of
co-solvents and time on the extraction yield of these compounds and their antioxidant activities for a possible applications
in food, cosmetic or pharmaceutical industries.
Adaptive multibeam laser cutting of thin steel sheets with fiber laser using spatial light modulator
Authors: Kaakkunen Jarno J. J. , Laakso Petri , Kujanpää Veli
Published: June 2014

Laser cutting with multibeam diffraction pattern using an adaptive optics is studied. Infrared (IR) continuous single-mode fiber laser with maximum power of 200 W is applied to laser cutting of thin metal sheets, together with liquid crystal on silicon spatial light modulator (SLM). SLM can be used as the adaptive diffractive optical element, which can modify intensity profile of the laser beam almost arbitrary. In studies single Gaussian laser beam was divided into multiple spots. It was found out that with this way it is possible to enhance the cutting quality and also decrease pressure of the needed assist gas. Multibeam configuration enabled to laser cut burr free with 50% less protection gas pressure compared to single beam laser cutting with conventional optics.
Histological Profile of the Longissimus Dorsi Muscle in Polish Large White and Polish Landrace Pigs and its Effect on Loin Parameters and Intramuscular Fat Content*
Authors: Bereta Anna , Tyra Mirosław , Ropka-Molik Katarzyna , Wojtysiak Dorota , Różycki Marian , Eckert Robert
Published: June 2014

The objective of this study was to analyse differences in individual fibre types in the histological profile of the longissimus dorsi muscle and their effect on pork carcass lean content and level of intramuscular fat (IMF) content, which determines palatability of meat and meat products. Analysis showed that the amount of type IIB fibres had a statistically significant (P<0.05) effect on the IMF content of the longissimus dorsi muscle. Animals with more than 70% of type IIB fibres in this muscle were also characterized by larger loin eye area (P<0.01) and loin eye height (P<0.05). Analogous relationships were noted when the analysed group of animals was divided according to the diameter of type IIA fibres. IMF was negatively correlated to the percentage of type IIB fibres (rP= -0.162). Relationships with the other two fibre types were positive (IIA - rP= 0.097; I - rP= 0.187). It was found that increased percentage of type IIB fibres resulted in a slightly greater loin weight (rP= 0.176), higher loin eye height (rP= 0.136), larger loin eye area (rP= 0.265) and higher carcass lean content (rP= 0.204). Likewise, the increase in the number of type IIA and type I fibres decreased these parameters.
Changes in Domestic Animals After Endotoxin Administration a Review
Authors: Danek Janusz , Żurek Urszula
Published: June 2014

gram-negative bacteria. Lipopolysaccharides are complex glycolipids composed of lipid A, a core polysaccharide, and repeating units of O-specific polysaccharides. Lipid A is associated with the majority of toxic effects of gram-negative bacteria. It interacts with target cells, such as macrophages and activated neutrophils, initiating the inflammatory cascade. The effects of LPS depend mostly on organism reaction to endotoxins, their concentration in blood and also on the state of the animal before the occurrence of endotoxemia. The effect of endotoxin on the reproductive functions of domestic animals has been partly elucidated. This paper presents data on clinical, hematological and biochemical changes in endotoxemia as well as effects of injection of endotoxin in the reproductive systems in boars, rams, and stallions. The results show that these males respond to endotoxins in a similar way, concerning clinical symptoms, body temperature and leukocyte count in blood. Endocrine and seminal changes are different and the range of these changes also depends on the dose and endotoxin exposure time.
Atmospheric Circulation Influence on the Winter Thermal Conditions in Poland in 2021-2050 Based on the RACMO2 Model
Authors: Jędruszkiewicz Joanna , Piotrowski Piotr
Published: June 2014

Thermal conditions are largely determined by atmospheric circulation. Therefore, projection of future temperature changes should be considered in relation to changes in circulation patterns. This paper assess to what extent changes in circulation correspond to spatial variability of the winter temperature increase in Poland in 2021-2050 period based on the RACMO2 model. The daily data of the mean temperature and sea level pressure (SLP) from selected regional climate model and observations were used. SLP data were used to determine the advection types and circulation character. Firstly, changes in frequency of circulation types between 2021-2050 and 1971-2000 periods were examined. Then changes in air temperature for specific circulation type in relation to reference period were studied. Finally, the influence of atmospheric circulation on spatial temperature variation was discussed. Considerably high increase in cyclonic situation of more than 18%, especially from the west and south-west direction, and decrease in anticyclonic situation mainly from the west and northwest in winter was noticed. Changes in frequency of circulation types result in temperature growth. For some types it is predicted that warming can reach even 3-4°C. The cyclonic (Ec, SEc, Sc) and anticylonic (SEa, Sa, Ea) types are likely to foster the highest warming in the scenario period.
The Use of 3D Graphic Modelling in Geoarchaeological Investigations (Bykowszczyzna Archaeological Site near Kock, E Poland)
Authors: Łojek Jacek
Published: June 2014

The objective of this paper was to use the ArcView 3.2 application for spatial modelling of the exploration forms (pits) in the Bykowszczyzna 8 archaeological site. The 3D digital documentation at a specific scale makes possible easy archiving, presentation, and simple spatial analyses of the examined objects. The ArcView 3.2 programme and its extensions (Spatial Analyst and 3D Analyst), commonly used as analytical tools in geomorphology, were inventively used for inventory-making in the archaeological site. Traditional field sketches were only a base, which enables entering data into the programme, and don’t documentation material in itself as it used to be. The method of data visual ization proposed by the author gives new possibilities for using the GIS platform software.
Geochemical Methods of Inference the Thermoregulatory Strategies in Middle Triassic Marine Reptiles – A Pilot Study
Authors: Surmik Dawid , Pelc Andrzej
Published: June 2014

The oxygen stable isotopes investigation to elucidate thermoregulatory strategies in Middle Triassic basal sauropterygians is currently ongoing at University of Silesia and University of Maria Curie-Skłodowska. The results of similar studies on Late Mesozoic marine reptiles indicate that some of fully aquatic reptiles like plesiosaurs or ichthyosaurs could be warm-blooded animals.

Our investigation is an important part of the aim of the research project "The Marine and Terrestrial reptiles in the Middle Triassic environmental background of Southern Poland" to solve the thermoregulation issue in basal marine reptiles and show how, and when did homoiothermy evolve in Sauropterygia..

Homeothermy and gigantothermy were important physiological adaptations which allowed sauropterygian ancestors to leave the shores and conquer the open seas and oceans.
Artificial General Intelligence: Concept, State of the Art, and Future Prospects
Authors: Goertzel Ben
Published: June 2014

In recent years broad community of researchers has emerged, focusing on the original ambitious goals of the AI field - the creation and study of software or hardware systems with general intelligence comparable to, and ultimately perhaps greater than, that of human beings. This paper surveys this diverse community and its progress. Approaches to defining the concept of Artificial General Intelligence (AGI) are reviewed including mathematical formalisms, engineering, and biology inspired perspectives. The spectrum of designs for AGI systems includes systems with symbolic, emergentist, hybrid and universalist characteristics. Metrics for general intelligence are evaluated, with a conclusion that, although metrics for assessing the achievement of human-level AGI may be relatively straightforward (e.g. the Turing Test, or a robot that can graduate from elementary school or university), metrics for assessing partial progress remain more controversial and problematic.
Improved Real-time Denoising Method Based on Lifting Wavelet Transform
Authors: Liu Zhaohua , Mi Yang , Mao Yuliang
Published: June 2014

Signal denoising can not only enhance the signal to noise ratio (SNR) but also reduce the effect of noise. In order to satisfy the requirements of real-time signal denoising, an improved semisoft shrinkage real-time denoising method based on lifting wavelet transform was proposed. The moving data window technology realizes the real-time wavelet denoising, which employs wavelet transform based on lifting scheme to reduce computational complexity. Also hyperbolic threshold function and recursive threshold computing can ensure the dynamic characteristics of the system, in addition, it can improve the real-time calculating efficiency as well. The simulation results show that the semisoft shrinkage real-time denoising method has quite a good performance in comparison to the traditional methods, namely soft-thresholding and hard-thresholding. Therefore, this method can solve more practical engineering problems.
Methods for Determination of Mean Speckle Size in Simulated Speckle Pattern
Authors: Hamarová I. , Šmíd P. , Horváth P. , Hrabovský M.
Published: June 2014

This paper deals with computation of mean speckle size in a speckle pattern generated through a numerical simulation of speckle after reflection of a Gaussian beam off a rough object’s surface. Within this simulation various speckle patterns are obtained by means of change in a parameter of the Gaussian beam. The mean speckle size is computed through two approaches using both the two-dimensional and the one-dimensional normalized autocorrelation function in intensity. Additionally, we propose a distinct optimization of the determination of the mean speckle size by reduction of intensity values representing detected speckle patterns. Results of the determination of the mean speckle size are compared with theoretical predictions
New Species of the Genus Altensteinia (Orchidaceae, Spiranthoideae) from Colombia
Authors: Nowak Sławomir , Szlachetko Dariusz L. , Mytnik-Ejsmont Joanna
Published: June 2014

A new species of Altensteinia Kunth, probably endemic to the Colombian Andes, is described, illustrated and discussed, with notes on its habitat and altitude. A dichotomous key for determination of Altensteinia species in Colombia is provided
Application of the generalized Prony spectrum for extraction of information hidden in chaotic trajectories of triple pendulum
Authors: Nigmatullin Raoul , Osokin Sergey , Awrejcewicz Jan , Kudra Grzegorz
Published: June 2014

In this paper we apply a new method of analysis of random behavior of chaotic systems based on the Prony decomposition. The generalized Prony spectrum (GPS) is used for quantitative description of a wide class of random functions when information about their probability distribution function is absent. The scaling properties of the random functions that keep their invariant properties on some range of scales help to fit the compressed function based on the Prony’s decomposition. In paper [1] the first author (RRN) found the physical interpretation of this decomposition that includes the conventional Fourier decomposition as a partial case. It has been proved also that the GPS can be used for detection of quasi-periodic processes that are appeared usually in the repeated or similar measurements. A triple physical pendulum is used as a chaotic system to obtain a chaotic behavior of displacement angles with one, two and three positive Lyapunov’s exponents (LEs). The chaotic behavior of these angles can be expressed in the form of amplitude-frequency response (AFR) that is extracted from the corresponding GPS and can serve as a specific ”fingerprint” characterizing the random behavior of the triple-pendulum system studied. This new quantitative presentation of random data opens additional possibilities in classification of chaotic responses and random behaviors of different complex systems.
Isotope analyses of the lake sediments in the Plitvice Lakes, Croatia
Authors: Horvatinčić Nada , Sironić Andreja , Barešić Jadranka , Bronić Ines , Nikolov Jovana , Todorović Nataša , Hansman Jan , Krmar Miodrag
Published: June 2014

The analyses of radioactive isotopes 14C, 137Cs and 210Pb, and stable isotope 13C were performed in the sediment cores, top 40 cm, taken in 2011 from karst lakes Prošće and Kozjak in the Plitvice Lakes National Park, central Croatia. Frozen sediment cores were cut into 1 cm thick layers and dried. 14C activity in both carbonate and organic fractions was measured using accelerator mass spectrometry technique with graphite synthesis. 137Cs, 210Pb, 214Pb and 214Bi were measured by low level gamma spectrometry method on ORTECHPGe detector with the efficiency of 32%.

Distribution of 14C activity from both lakes showed increase of the 14C activity in the top 10–12 cm in both carbonate and organic fractions as a response to thermonuclear bomb-produced 14C in the atmosphere in the sixties of the 20th century. Anthropogenically produced 137Cs was also observed in sediment profiles. Sedimentation rates for both lake sediments were estimated based on the unsupported 210Pb activity.

Different 14C activity of the carbonate fraction (63–80 pMC, percent of modern carbon) and organic fraction (82–93 pMC) is the result of geochemical and biological processes of the sediment precipitation in the lake waters. This is also confirmed by the δ
13
C values of both fractions. Carbon isotope composition, a
14
C and δ
13
C, was compared with the lake sediments from the same lakes collected in 1989 and 2003.
Two-phase fed-batch modification for 48 hour peak expression of hepatitis B surface antigen in Pichia pastoris shake flask system
Authors: Tam Yew , Zeenathul Nazariah , Morvarid Akhavan , Azmi Mohd , Bahaman Abdul , Lo Sewn , Tan Joo
Published: June 2014

A study of the Mut+ phenotype for the expression of recombinant hepatitis B surface antigen (HBsAg) in Pichia pastoris strain GS115 using the pPIC3.5K vector with a two-phase fed-batch protocol in a shake flask system is described. Expression levels of HBsAg protein of 6.74 g L−1 Dry Cell Weight (DCW) and 26.07 mg L−1 of HBsAg concentration were achieved 48 h from the induction point which added to a 120 h reduction in production period in comparison with MutS expression (168 h). The use of the pPIC3.5K-HBsAg plasmid in the Mut+ phenotype enhanced the expression of HBsAg by a nearly 13 times higher volumetric productivity in the first 24 h and 35 times higher at peak production concentration. Comparison of AOX expression cassettes relative to the HBsAg gene in the role of mRNA secondary structure during translation initiation revealed that HBsAg possesses lower folding stability with AOX1 Mut+ phenotype. The results from this study demonstrated that expression of HBsAg with Mut+
AOX1 promoter is adequate as an alternative for the production of HBsAg. In addition, the AOX promoter of the Mut+ phenotype was observed to be better suited for HBsAg expression, which correlates with the ease of translation initiation under shake flask conditions.
The expression of the Candida albicans gene SAP4 during hyphal formation in human serum and in adhesion to monolayer cell culture of colorectal carcinoma Caco-2 (ATCC)
Authors: Staniszewska Monika , Bondaryk Małgorzata , Malewski Tadeusz , Schaller Martin
Published: June 2014

Candida albicans SAP4 gene encodes secretory aspartyl protease Sap4 which is involved in hyphae formation and virulence. Transcriptional factors Cph1 and Efg1 govern the expression of several C. albicans genes and contribute to morphogenesis. We investigated the expression of SAP4 in C. albicans clinical isolate and mutants lacking Efg1 or/ and Cph1 grown in human serum and during contact with Caco-2 cell line. mRNA was analyzed with the use of RT-PCR; relative quantification was normalized against an ACT1 in cells after 18-h growth either in serum or on monolayer as well as in their counterparts in YEPD medium. We assessed the role of Sap4, Efg1 and Cph1 in adhesion of C. albicans to epithelial cells. Additionally, adherence assay was performed with sap4/sap4. Adhesion was expressed as a percent of adherent cells to monolayer at 90 min vs. total cells added (100%). No differences were observed in adhesion of efg1/efg1 and sap4/sap4 compared with SC5314 (P≥0.05 statisitically insignificant). SAP4 expression indicated that it is not involved in adapting to the tested conditions. SAP4 expression can be strainspecific and is not solely controlled by the Efg1 pathway but also by the Cph1 pathway. Neither Efg1 nor Sap4 can influence adhesion.
Effect of altered snow conditions on decomposition in three subalpine plant communities
Authors: Zeidler Miroslav , Duchoslav Martin , Banaš Marek
Published: June 2014

Snow cover and its spatio-temporal changes play a crucial role in the ecological functioning of mountains. Some human activities affecting snow properties may cause shifts in the biotic components of ecosystems, including decomposition. However, these activities remain poorly understood in subalpine environments. We explored the effect of human-modified snow conditions on cellulose decomposition in three vegetation types. Snow density, soil temperature, and the decomposition of cellulose were studied in Athyrium, Calamagrostis, and Vaccinium vegetation types, comparing stands intersected by groomed ski slope and natural (outside the ski slope) stands. Increased snow density caused the deterioration of snow insulation and decreased the soil temperature inside the ski slope only slightly in comparison with that outside the ski slope in all vegetation types studied. The decomposition was apparently lower in Athyrium vegetation relative to the other vegetation types and strongly (Athyrium vegetation) to weakly lower (other vegetation types) in groomed than in ungroomed stands. Wintertime, including the melting period, was decisive for overall decomposition. Our results suggest that differences in decomposition are influenced by ski slope operations and vegetation type. Alterations in snow conditions appeared to be subtle and long-term but with important consequences for conservation management.
DC Resistivity Studies of Shallow Geology in the Vicinity of Vistula River Flood Bank in Czernichów Village (Near Kraków in Poland)
Authors: Mościcki Włodzimierz Jerzy , Bania Grzegorz , Ćwiklik Michał , Borecka Aleksandra
Published: June 2014

DC resistivity methods, soundings and Electrical Resistivity Tomography, were applied to study shallow geology in the place of planned construction of an experimental flood bank. The geoelectrical surveys provided quantitative information about the spatial presence of the various geoelectrical/geological layers: alluvial soils, sands, gravels and clays. ERT allowed maps to be constructed showing subsurface structure. A combination of geoelectrical and geological information resulted in a much better identification of the geological structure.
Conditional simulation of Thwaites Glacier (Antarctica) bed topography for flow models: Incorporating inhomogeneous statistics and channelized morphology
Authors: Goff John A. , Powell Evelyn M. , Young Duncan A. , Blankenship Donald D.
Published: June 2014

Thwaites Glacier, Antarctica, is experiencing rapid change and its mass could, if disgorged into the ocean, lead to ∼1 m of global sea-level rise. Efforts to model flow for Thwaites Glacier are strongly dependent on an accurate model of bed topography. Airborne radar data collected in 2004/05 provide 35 000 line km of bed topography measurements sampled every 20 m along track. At ∼15 km track spacing, this extensive dataset nevertheless misses considerable important detail, particularly: (1) resolution of mesoscale channelized morphology that can guide glacier flow; and (2) resolution of small-scale roughness between the track lines that is critical for determining topographic resistance to flow. Both issues are addressed using a conditional simulation that merges a stochastic realization (an unconditional simulation) with a deterministic surface. A conditional simulation is a non-unique interpolation that reproduces observed statistical behavior without affecting data values. Channels are resolved in the deterministic surface using an interpolation algorithm designed for sinuous channels. Small-scale roughness is resolved using a statistical analysis that accounts for heterogeneity, including an abrupt transition between ‘lowland’ and ‘highland’ morphology. Multiple realizations of the unconditional simulation can be generated to sample the probability space and allow error characterization in flow modeling.
Untreated Pain a 'Scandal of Global Proportions'
Authors: Doherty Megan
Published: June 2014

DOI: http://dx.doi.org/10.3329/bjms.v13i3.17694 Bangladesh Journal of Medical Science Vol.13(3) 2014 p.249-250
Analysis of brain tumors in Kashmir Valley - A 10 year study
Authors: Dhar Anil , Bhat Abdul Rashid , Nizami Furqan A , Kirmani A R , Zargar Javeed , Ramzan A U , Wani M A
Published: June 2014

Background: Geographically Kashmir valley is isolated from the rest of the country. It has a different climate with people having different social and dietary habits. Gastric cancer, esophageal, and skin (Kangri) cancer have a higher prevalence but there is little data available on the cancers of brain.Objectives & Methodology: Aim was to study brain tumors prospectively and retrospectively, to analyse brain tumors geographically and to analyse the age and sex ratio of brain tumors in Kashmir valley. In this Retrospective and Prospective study, retrospectively (initial seven years) all patients were analyzed for their clinical symptoms, age, sex, residence, histopathologic characteristics of tumors. Prospectively (later three years) after get-ting the radiological diagnosis pathological diagnosis was arrived by procedures like open, stereotactic, and endoscopic procedures. All patients were then analysed for age, sex, residence, signs and symptoms and histopathological characteristics. Follow up was done for gliomas. Mortality and morbidity was analysed for gliomas in these 3 years. Patients who lost the follow up were considered dead. Out of 1730 patients included in our study, there were 1031 males and 699 females. The most common age group was between 41-50 years. Results: The most common tumor was gliomas followed by meningiomas. Gliomas were most common in men and meningiomas in females.  Out of all the histological grades in gliomas, the glioblastoma multiforme (GBM) was the most common, and frontal lobe was the commonest anatomical site involved. The most common symptom in our study was headache followed by vomiting.DOI: http://dx.doi.org/10.3329/bjms.v13i3.19148Bangladesh Journal of Medical Science Vol.13(3) 2014 p.268-277
Reduction of CD4 count induces opportunistic infections in people living with HIV (PLHIV)
Authors: Noor Rashed , Morsalin Md. , Chakraborty Bidhan
Published: June 2014

Objective: Human immunodeficiency virus (HIV), a lentivirus (member of the retrovirus family) causing acquired immunodeficiency syndrome (AIDS), weakens the immune system of the body and hence associates different opportunistic infections. Present study undertook a survey on opportunistic infections. Materials and Methods: Data were collected from both HIV carriers (CD4 count more than 250/mL of blood) and AIDS patients (CD4 count less than 250/mL of blood). Results: Analyses of the data revealed that diarrhoea, pulmonary tuberculosis, gland tuberculosis, skin lesions and fever were the common opportunistic infections. Conclusion: It can be summarized that HIV infected patients having a reduced CD4 count (<250/mL) encounter different opportunistic infections and some of these infections could be continual for long as well. DOI: http://dx.doi.org/10.3329/bjms.v13i3.19151 Bangladesh Journal of Medical Science Vol.13(3) 2014 p.285-291
Clinico-Histopathological histopathological correlation and detection of anti PGL-1 antibodies in leprosy patients
Authors: Datta Soma , Pandya SP , Marfatia YR , Rakshit Arindam
Published: June 2014

Background: Leprosy (Hansens disease) is one of the major health problems of the world especially in developing countries. In India, it was first described in Sushruta Samhita & treated by Chaulmoogra oil and caused by Mycobacterium Leprae. Early diagnosis of leprosy, an absolute necessity for control as well as effective therapy. For this, clinical diagnosis, skin smear examination is adequate coupled with histo-pathological examination of skin and nerve lesions with modified Fite Faraco stain for demonstration of acid-fast bacilli. Moreover, bacillary index is required for adequate combined chemotherapy regimen. Detection of anti PGL-1 antibodies in serum gives an added advantage for detection and monitoring treatment. Materials & Methods : A total of 85 cases of leprosy who attended outpatient department of Skin & VD, Shri Sayaji General Hospital Baroda chosen for study during 07-08 period with 75 cases from leprosy hospital, Baroda which included 50 detected patients and 25 child contacts with 25 healthy voluntary blood donors from blood bank, SSGH selected. Clinical, past and family history taken with slit skin smears stained with Z-N stain, graded and histopathological evaluation done. Serological study done from serum of leprosy patients and healthy blood donors; tested by Serodia Kitsand interpretation made. Results: Most cases were in 2nd to 4th decade and males dominated. Clinically most cases were of indeterminate and tuberculoid type and histologically indeterminate and border-line tuberculoid. Clinico-histopathological correlation was found most in indeterminate followed by histoid type. Voluntary blood donors were seronegative. 21 out of 48 multibacillary cases and 6 out of 28 pauci-bacillary showed seropositivity for anti PGL-1 anti-bodies (p<0.001) Conclusion: All suspected leprosy cases clinically should be subjected to slit skin smear examination with histopathological evaluation; bacillary study which helps in diagnosis and adequate treatment of patients. Detection of antibodies to PGL-1 in patients indicate presence of leprosy bacilli and useful in preclinical diagnosis and determining progress of therapy. DOI: http://dx.doi.org/10.3329/bjms.v13i3.9153 Bangladesh Journal of Medical Science Vol.13(3) 2014 p.316-322
Community in ‘Global’ Academies: The Critical Positioning of ‘Meta-Francophone’ Caribbeanists
Authors: Gallagher Mary
Published: June 2014

Caribbeanists working on the Francophone Caribbean within the Anglophone academy are perhaps particularly well placed to bring into focus the linguistic and cultural losses of the dislocations and relocations of High Capitalism. Although our object of study should facilitate critical insights into the fundamental linguistic and cultural indifference and irresponsibility of capitalist extraction models and into what is at stake politically and ethically in contemporary versions of those profiteering models, the commercialist reductionism currently (re-)formatting not just our own pulverized academic universe, but the entire globe over which it has by now comprehensively spread, surely works against any such truly critical positioning.
Myogenic Differential Methylation: Diverse Associations with Chromatin Structure
Authors: Chandra Sruti , Baribault Carl , Lacey Michelle , Ehrlich Melanie
Published: June 2014

Employing a new algorithm for identifying differentially methylated regions (DMRs) from reduced representation bisulfite sequencing profiles, we identified 1972 hypermethylated and 3250 hypomethylated myogenic DMRs in a comparison of myoblasts (Mb) and myotubes (Mt) with 16 types of nonmuscle cell cultures. DMRs co-localized with a variety of chromatin structures, as deduced from ENCODE whole-genome profiles. Myogenic hypomethylation was highly associated with both weak and strong enhancer-type chromatin, while hypermethylation was infrequently associated with enhancer-type chromatin. Both myogenic hypermethylation and hypomethylation often overlapped weak transcription-type chromatin and Polycomb-repressed-type chromatin. For representative genes, we illustrate relationships between DNA methylation, the local chromatin state, DNaseI hypersensitivity, and gene expression. For example, MARVELD2 exhibited myogenic hypermethylation in transcription-type chromatin that overlapped a silenced promoter in Mb and Mt while TEAD4 had myogenic hypomethylation in intronic subregions displaying enhancer-type or transcription-type chromatin in these cells. For LSP1, alternative promoter usage and active promoter-type chromatin were linked to highly specific myogenic or lymphogenic hypomethylated DMRs. Lastly, despite its myogenesis-associated expression, TBX15 had multiple hypermethylated myogenic DMRs framing its promoter region. This could help explain why TBX15 was previously reported to be underexpressed and, unexpectedly, its promoter undermethylated in placentas exhibiting vascular intrauterine growth restriction.
Solar Air Collectors for Space Heating and Ventilation Applications—Performance and Case Studies under Romanian Climatic Conditions
Authors: Budea Sanda
Published: June 2014

Solar air collectors have various applications: on the one hand, they can be used for air heating in cold seasons; on the other hand they can be used in summer to evacuate the warm and polluted air from residential, offices, industrial, and commercial buildings. The paper presents experimental results of a solar collector air, under the climatic conditions of the Southeastern Europe. The relationships between the direct solar irradiation, the resulting heat flow, the air velocity at the outlet, the air flow rate, the nominal regime of the collector and the efficiency of conversion of solar energy into thermal energy are all highlighted. Thus, it was shown that after a maximum 50 min, solar air collectors, with baffles and double air passage can reach over 50% efficiency for solar irradiation of 900–1000 W/m2. The article also presents a mathematical model and the results of a computational program that allows sizing solar collectors for the transfer of air, with the purpose of improving the natural ventilation of buildings. The article is completed with case studies, sizing the area to be covered with solar collectors, to ensure ventilation of a house with two floors or for an office building. In addition, the ACH (air change per hour) coefficient was calculated and compared.
Humidity and Gravimetric Equivalency Adjustments for Nephelometer-Based Particulate Matter Measurements of Emissions from Solid Biomass Fuel Use in Cookstoves
Authors: Soneja Sutyajeet , Chen Chen , Tielsch James , Katz Joanne , Zeger Scott , Checkley William , Curriero Frank , Breysse Patrick
Published: June 2014

Great uncertainty exists around indoor biomass burning exposure-disease relationships due to lack of detailed exposure data in large health outcome studies. Passive nephelometers can be used to estimate high particulate matter (PM) concentrations during cooking in low resource environments. Since passive nephelometers do not have a collection filter they are not subject to sampler overload. Nephelometric concentration readings can be biased due to particle growth in high humid environments and differences in compositional and size dependent aerosol characteristics. This paper explores relative humidity (RH) and gravimetric equivalency adjustment approaches to be used for the pDR-1000 used to assess indoor PM concentrations for a cookstove intervention trial in Nepal. Three approaches to humidity adjustment performed equivalently (similar root mean squared error). For gravimetric conversion, the new linear regression equation with log-transformed variables performed better than the traditional linear equation. In addition, gravimetric conversion equations utilizing a spline or quadratic term were examined. We propose a humidity adjustment equation encompassing the entire RH range instead of adjusting for RH above an arbitrary 60% threshold. Furthermore, we propose new integrated RH and gravimetric conversion methods because they have one response variable (gravimetric PM2.5 concentration), do not contain an RH threshold, and is straightforward.
Female Moth Calling and Flight Behavior Are Altered Hours Following Pheromone Autodetection: Possible Implications for Practical Management with Mating Disruption
Authors: Stelinski Lukasz , Holdcraft Robert , Rodriguez-Saona Cesar
Published: June 2014

Female moths are known to detect their own sex pheromone—a phenomenon called “autodetection”. Autodetection has various effects on female moth behavior, including altering natural circadian rhythm of calling behavior, inducing flight, and in some cases causing aggregations of conspecifics. A proposed hypothesis for the possible evolutionary benefits of autodetection is its possible role as a spacing mechanism to reduce female-female competition. Here, we explore autodetection in two species of tortricids (Grapholita molesta (Busck) and Choristoneura rosaceana (Harris)). We find that females of both species not only “autodetect,” but that learning (change in behavior following experience) occurs, which affects behavior for at least 24 hours after pheromone pre-exposure. Specifically, female calling in both species is advanced at least 24 hours, but not 5 days, following pheromone pre-exposure. Also, the propensity of female moths to initiate flight and the duration of flights, as quantified by a laboratory flight mill, were advanced in pre-exposed females as compared with controls. Pheromone pre-exposure did not affect the proportion of mated moths when they were confined with males in small enclosures over 24 hours in laboratory assays. We discuss the possible implications of these results with respect to management of these known pest species with the use of pheromone-based mating disruption.
Pyrazines Attract Catocheilus Thynnine Wasps
Authors: Bohman Bjorn , Peakall Rod
Published: June 2014

Five previously identified semiochemicals from the sexually deceptive Western Australian hammer orchid Drakaea livida, all showing electrophysiological activity in gas chromatography–electroantennogram detection (EAD) studies, were tested in field bioassays as attractants for a Catocheilus thynnine wasp. Two of these compounds, (3,5,6-trimethylpyrazin-2-yl)methyl 3-methylbutanoate and 2-(3-methylbutyl)-3,5,6-trimethylpyrazine, were attractive to male wasps. Additionally, the semiochemical 3-(3-methylbutyl)-2,5-dimethylpyrazine, a close analogue to 2-(3-methylbutyl)-3,5,6-trimethylpyrazine, identified in five other species of thynnine wasps, was equally active. The three remaining compounds from D. livida, which were EAD-active against Catocheilus, did not attract the insects in field trials. It is interesting that two structurally similar compounds induce similar behaviours in field experiments, yet only one of these compounds is present in the orchid flower. Our findings suggest the possibility that despite the high specificity normally characterising sex pheromone systems, the evolution of sexual deception may not be entirely constrained by the need to precisely match the sex pheromone constituents and blends. Such evolutionary flexibility may be particularly important during the early stages of speciation.
Optimal Pharmacologic Treatment Strategies in Obesity and Type 2 Diabetes
Authors: Goswami Gayotri , Shinkazh Nataliya , Davis Nichola
Published: June 2014

The prevalence of obesity has increased to pandemic levels worldwide and is related to increased risk of morbidity and mortality. Metabolic comorbidities are commonly associated with obesity and include metabolic syndrome, pre-diabetes, and type 2 diabetes. Even if the prevalence of obesity remains stable until 2030, the anticipated numbers of people with diabetes will more than double as a consequence of population aging and urbanization. Weight reduction is integral in the prevention of diabetes among obese adults with pre-diabetes. Lifestyle intervention and weight reduction are also key in the management of type 2 diabetes. Weight loss is challenging for most obese patients, but for those with diabetes, it can pose an even greater challenge due to the weight gain associated with many treatment regimens. This article will review optimal treatment strategies for patients with comorbid obesity and type 2 diabetes. The role of anti-obesity agents in diabetes will also be reviewed. This literature review will provide readers with current strategies for the pharmacologic treatment of obesity and diabetes with a focus on the weight outcomes related to diabetes treatments.
It’s More Than a Blood Test: Patients’ Perspectives on Noninvasive Prenatal Testing
Authors: Farrell Ruth , Mercer Mary , Agatisa Patricia , Smith Marissa , Philipson Elliot
Published: June 2014

Noninvasive prenatal testing (NIPT) offers pregnant women a new risk assessment tool for fetal aneuploidy that is superior to conventional screening tests. We conducted focus groups with women who were currently pregnant or had recently delivered in the past year to characterize their perspectives about NIPT and to explore factors they would consider during decision making about its use. Women identified accuracy, early timing, testing ease, and determination of fetal sex as advantages of NIPT over other screens, and the noninvasive method of NIPT as an advantage over diagnostic tests. False positive and false negative results, anxiety, cost and insurance coverage were seen as disadvantages of NIPT. Women who do not want fetal aneuploidy information most likely will not undergo NIPT, despite its advantages over other screening tests. However, given its advantages, the decision to have NIPT is straightforward for women who want genetic information about the fetus. Women emphasized the need to make autonomous, private, and informed choices about NIPT, as they would with any prenatal genetic testing option. These perspectives may guide clinicians to conduct effective and clinically relevant counseling with pregnant women who consider utilizing this new genetic technology.
The Micropillar Structure on Silk Fibroin Film Influence Intercellular Connection Mediated by Nanotubular Structures
Authors: You Renchuan , Li Xiufang , Xu Yamei , Liu Yu , Lu Shenzhou , Li Mingzhong
Published: June 2014

Tunneling nanotubes are important membrane channels for cell-to-cell communication. In this study, we investigated the effect of the microenvironment on nanotubular structures by preparing a three-dimensional silk fibroin micropillar structure. In previous reports, tunneling nanotubes were described as stretched membrane channels between interconnected cells at their nearest distance. They hover freely in the cell culture medium and do not contact with the substratum. Interestingly, the micropillars could provide supporting points for nanotubular connection on silk fibroin films, where nanotubular structure formed a stable anchor at contact points. Consequently, the extension direction of nanotubular structure was affected by the micropillar topography. This result suggests that the hovering tunneling nanotubes in the culture medium will come into contact with the raised roadblock on the substrates during long-distance extension. These findings imply that the surface microtopography of biomaterials have an important influence on cell communication mediated by tunneling nanotubes.
Improved Strength and Toughness of Carbon Woven Fabric Composites with Functionalized MWCNTs
Authors: Soliman Eslam , Kandil Usama , Taha Mahmoud
Published: June 2014

This investigation examines the role of carboxyl functionalized multi-walled carbon nanotubes (COOH-MWCNTs) in the on- and off-axis flexure and the shear responses of thin carbon woven fabric composite plates. The chemically functionalized COOH-MWCNTs were used to fabricate epoxy nanocomposites and, subsequently, carbon woven fabric plates to be tested on flexure and shear. In addition to the neat epoxy, three loadings of COOH-MWCNTs were examined: 0.5 wt%, 1.0 wt% and 1.5 wt% of epoxy. While no significant statistical difference in the flexure response of the on-axis specimens was observed, significant increases in the flexure strength, modulus and toughness of the off-axis specimens were observed. The average increase in flexure strength and flexure modulus with the addition of 1.5 wt% COOH-MWCNTs improved by 28% and 19%, respectively. Finite element modeling is used to demonstrate fiber domination in on-axis flexure behavior and matrix domination in off-axis flexure behavior. Furthermore, the 1.5 wt% COOH-MWCNTs increased the toughness of carbon woven composites tested on shear by 33%. Microstructural investigation using Fourier Transform Infrared Spectroscopy (FTIR) proves the existence of chemical bonds between the COOH-MWCNTs and the epoxy matrix.
Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers
Authors: Ronkainen Niina , Okon Stanley
Published: June 2014

Nanotechnology has played a crucial role in the development of biosensors over the past decade. The development, testing, optimization, and validation of new biosensors has become a highly interdisciplinary effort involving experts in chemistry, biology, physics, engineering, and medicine. The sensitivity, the specificity and the reproducibility of biosensors have improved tremendously as a result of incorporating nanomaterials in their design. In general, nanomaterials-based electrochemical immunosensors amplify the sensitivity by facilitating greater loading of the larger sensing surface with biorecognition molecules as well as improving the electrochemical properties of the transducer. The most common types of nanomaterials and their properties will be described. In addition, the utilization of nanomaterials in immunosensors for biomarker detection will be discussed since these biosensors have enormous potential for a myriad of clinical uses. Electrochemical immunosensors provide a specific and simple analytical alternative as evidenced by their brief analysis times, inexpensive instrumentation, lower assay cost as well as good portability and amenability to miniaturization. The role nanomaterials play in biosensors, their ability to improve detection capabilities in low concentration analytes yielding clinically useful data and their impact on other biosensor performance properties will be discussed. Finally, the most common types of electroanalytical detection methods will be briefly touched upon.
Surveillance of C-Allocation in Microalgal Cells
Authors: Wagner Heiko , Jungandreas Anne , Fanesi Andrea , Wilhelm Christian
Published: June 2014

When microalgae are exposed to changing environmental conditions, e.g., light-dark cycles or oscillations in nutrient availability (CO2, nitrogen, phosphate or silicate) they respond with metabolic changes in the carbon allocation pattern. Short time regulations in the time range of few seconds to minutes can be mirrored best by mass spectroscopy based metabolomics. However, these snap shots do not reflect the alterations in the carbon flow to the cellular macromolecules like protein, carbohydrate or lipid. In this review it is shown how the combination of FTIR spectroscopy and Chla-in-vivo-fluorescence based electron transport rates can reveal changes in the metabolic flux rates of carbon during a shift of the environmental conditions. The review will demonstrate in which time range FTIR spectroscopy can deliver significant information and how FTIR spectroscopy data can synergistically support metabolome analysis by mass-spectroscopy.