bluuebunny/crossref_metadata_2025 huggingface dataset:
A Logic-Tree Approach for Probabilistic Seismic Hazard Assessment in the Administrative Region of Attica (Greece)
Authors: Kaviris George , Zymvragakis Angelos , Bonatis Pavlos , Kapetanidis Vasilis , Spingos Ioannis , Mavroulis Spyridon , Kotsi Evelina , Lekkas Efthymios , Voulgaris Nicholas
Published: June 2023

Probabilistic Seismic Hazard Assessment (PSHA) was carried out for the administrative region of Attica (Greece). Peak Ground Acceleration (PGA) and Peak Ground Velocity (PGV) values were calculated for return periods of 475 and 950 years for five sub-areas covering the entire region. PGA hazard curves and Uniform Hazard Spectra (UHS) in terms of spectral acceleration (Sa) values were generated for Athens, Methana, and the capitals of each island of Attica (Salamina, Aegina, Poros, Hydra, Spetses, Kythira, and Antikythira). Area sources were adopted from the Euro-Mediterranean Seismic Hazard Model 2013 (ESHM13) and its update, ESHM20, taking into account both crustal and slab tectonic environments. Ground Motion Prediction Equations (GMPEs) proposed for the Greek territory were ranked for PGA and PGV. Each GMPE was reconstructed as a weighted model, accounting for normal and non-normal focal mechanisms for each area source. PGA, PGV, and Sa values were computed using a logic tree, integrating the seismotectonic models as major branches and sub-logic trees, comprised of multiple ranked GMPEs for each area source, as minor branches. The results showed higher seismic hazard values in sub-areas near the Gulf of Corinth and the slab interface, which could indicate a need to revise the active building code in Attica.
Assessment of the Impact of Climate Change on Streamflow and Sediment in the Nagavali and Vamsadhara Watersheds in India
Authors: Nagireddy Nageswara Reddy , Keesara Venkata Reddy , Venkata Rao Gundapuneni , Sridhar Venkataramana , Srinivasan Raghavan
Published: June 2023

Climate-induced changes in precipitation and temperature can have a profound impact on watershed hydrological regimes, ultimately affecting agricultural yields and the quantity and quality of surface water systems. In India, the majority of the watersheds are facing water quality and quantity issues due to changes in the precipitation and temperature, which requires assessment and adaptive measures. This study seeks to evaluate the effects of climate change on the water quality and quantity at a regional scale in the Nagavali and Vamsadhara watersheds of eastern India. The impact rainfall variations in the study watersheds were modeled using the Soil and Water Assessment Tool (SWAT) with bias-corrected, statistically downscaled models from Coupled Model Intercomparison Project-6 (CMIP-6) data for historical (1975–2014), near future (2022–2060), and far future (2061–2100) timeframes using three Shared Socioeconomic Pathways (SSP) scenarios. The range of projected changes in percentage of mean annual precipitation and mean temperature varies from 0 to 41.7% and 0.7 °C to 2.7 °C in the future climate, which indicates a warmer and wetter climate in the Nagavali and Vamsadhara watersheds. Under SSP245, the average monthly changes in precipitation range from a decrease of 4.6% to an increase of 25.5%, while the corresponding changes in streamflow and sediment yield range from −11.2% to 41.2% and −15.6% to 44.9%, respectively. Similarly, under SSP370, the average monthly change in precipitation ranges from −3.6% to 36.4%, while the corresponding changes in streamflow and sediment yield range from −21.53% to 77.71% and −28.6% to 129.8%. Under SSP585, the average monthly change in precipitation ranges from −2.5% to 60.5%, while the corresponding changes in streamflow and sediment yield range from −15.8% to 134.4% and −21% to 166.5%. In the Nagavali and Vamsadhara watersheds, historical simulations indicate that 2438 and 5120 sq. km of basin areas, respectively, were subjected to high soil erosion. In contrast, under the far future Cold-Wet SSP585 scenario, 7468 and 9426 sq. km of basin areas in the Nagavali and Vamsadhara watersheds, respectively, are projected to experience high soil erosion. These results indicate that increased rainfall in the future (compared to the present) will lead to higher streamflow and sediment yield in both watersheds. This could have negative impacts on soil properties, agricultural lands, and reservoir capacity. Therefore, it is important to implement soil and water management practices in these river basins to reduce sediment loadings and mitigate these negative impacts.
Experimental Research on High-Temperature Stability of Asphalt Concrete Panels of Impermeable Layers
Authors: Han Qunzhu , Song Ge , Wang Yueyan , Zhang Yingbo , Ning Zhiyuan
Published: June 2023

In order to study the slope stability of an impervious layer asphalt concrete panel, in this study, the maximum aggregate size used was 19 mm, and a slope flow value test was carried out after changing the gradation index, filler content and bitumen aggregate ratio. The test results showed that the relationship curve between the slope flow value and the test time was mainly divided into three stages for the slope flow value: an almost linear growth stage, a gradual stabilization stage, and a stable stage. The grading index, bitumen aggregate ratio and filler content had an effect on the slope flow value of asphalt concrete. The slope flow value decreased with the increase in the grading index. A reasonable increase in the grading index can increase the slope stability of the asphalt concrete panel. The slope flow value increased with the increase in the filler content and bitumen aggregate ratio. When the filler content exceeded 13%, the slope flow value significantly increased. At the same time, it was also verified that the asphalt concrete slope with the maximum aggregate size of 19 mm had good thermal stability. On this basis, a prediction model of asphalt concrete slope flow value and test time was established. The model considered the effect of different parameters of mix proportion on the slope flow value. The calculation results were in good agreement with the test results.
Multi-Strain Epidemic Models of Mutating Viruses with Airborne Transmission Based on Cellular Automata and Ordinary Differential Equations
Authors: Schimit Pedro H. T.
Published: June 2023

The emergence of SARS-CoV-2 virus mutations is a pressing concern in the context of the COVID-19 pandemic. In this paper, a Susceptible–Infected–Recovered (SIR) compartmental model has been formulated in terms of cellular automata and ordinary differential equations to simulate the transmission dynamics of a virus. This model accounts for the potential of new virus mutations to create additional infections of a multi-strain disease while also considering the impact of vaccination on disease control within a population. The basic reproduction number of the disease is derived, and the effect of virus mutations and vaccination rates is evaluated on a population over five years. The results of numerical simulations demonstrate the significant role of maintaining high vaccination rates in controlling the spread of the virus, even when assuming that all variants have similar illness characteristics and that a single shot of vaccine provides complete and lifelong protection against all strains. The findings underscore the necessity for countries to implement a consistent and high-level vaccination plan as soon as vaccines become available in order to mitigate the impact of a pandemic effectively. In conclusion, this study highlights the importance of taking into account the potential impact of virus mutations for controlling the COVID-19 pandemic. Furthermore, it emphasizes the critical role of vaccination in limiting the spread of the virus, and emphasizes the need to implement and maintain high vaccination rates as part of a comprehensive approach to managing the ongoing pandemic.
The Use of Tactile Sensors in Oral and Maxillofacial Surgery: An Overview
Authors: Navalesi Pietro , Oddo Calogero Maria , Chisci Glauco , Frosolini Andrea , Gennaro Paolo , Abbate Vincenzo , Prattichizzo Domenico , Gabriele Guido
Published: June 2023

Background: This overview aimed to characterize the type, development, and use of haptic technologies for maxillofacial surgical purposes. The work aim is to summarize and evaluate current advantages, drawbacks, and design choices of presented technologies for each field of application in order to address and promote future research as well as to provide a global view of the issue. Methods: Relevant manuscripts were searched electronically through Scopus, MEDLINE/PubMed, and Cochrane Library databases until 1 November 2022. Results: After analyzing the available literature, 31 articles regarding tactile sensors and interfaces, sensorized tools, haptic technologies, and integrated platforms in oral and maxillofacial surgery have been included. Moreover, a quality rating is provided for each article following appropriate evaluation metrics. Discussion: Many efforts have been made to overcome the technological limits of computed assistant diagnosis, surgery, and teaching. Nonetheless, a research gap is evident between dental/maxillofacial surgery and other specialties such as endovascular, laparoscopic, and microsurgery; especially for what concerns electrical and optical-based sensors for instrumented tools and sensorized tools for contact forces detection. The application of existing technologies is mainly focused on digital simulation purposes, and the integration into Computer Assisted Surgery (CAS) is far from being widely actuated. Virtual reality, increasingly adopted in various fields of surgery (e.g., sino-nasal, traumatology, implantology) showed interesting results and has the potential to revolutionize teaching and learning. A major concern regarding the actual state of the art is the absence of randomized control trials and the prevalence of case reports, retrospective cohorts, and experimental studies. Nonetheless, as the research is fast growing, we can expect to see many developments be incorporated into maxillofacial surgery practice, after adequate evaluation by the scientific community.
In Vitro Analysis of Human Cartilage Infiltrated by Hydrogels and Hydrogel-Encapsulated Chondrocytes
Authors: Köck Hannah , Striegl Birgit , Kraus Annalena , Zborilova Magdalena , Christiansen Silke , Schäfer Nicole , Grässel Susanne , Hornberger Helga
Published: June 2023

Osteoarthritis (OA) is a degenerative joint disease causing loss of articular cartilage and structural damage in all joint tissues. Given the limited regenerative capacity of articular cartilage, methods to support the native structural properties of articular cartilage are highly anticipated. The aim of this study was to infiltrate zwitterionic monomer solutions into human OA-cartilage explants to replace lost proteoglycans. The study included polymerization and deposition of methacryloyloxyethyl-phosphorylcholine- and a novel sulfobetaine-methacrylate-based monomer solution within ex vivo human OA-cartilage explants and the encapsulation of isolated chondrocytes within hydrogels and the corresponding effects on chondrocyte viability. The results demonstrated that zwitterionic cartilage–hydrogel networks are formed by infiltration. In general, cytotoxic effects of the monomer solutions were observed, as was a time-dependent infiltration behavior into the tissue accompanied by increasing cell death and penetration depth. The successful deposition of zwitterionic hydrogels within OA cartilage identifies the infiltration method as a potential future therapeutic option for the repair/replacement of OA-cartilage extracellular suprastructure. Due to the toxic effects of the monomer solutions, the focus should be on sealing the OA-cartilage surface, instead of complete infiltration. An alternative treatment option for focal cartilage defects could be the usage of monomer solutions, especially the novel generated sulfobetaine-methacrylate-based monomer solution, as bionic for cell-based 3D bioprintable hydrogels.
Autism Spectrum Disorder: A Neuro-Immunometabolic Hypothesis of the Developmental Origins
Authors: Frasch Martin G. , Yoon Byung-Jun , Helbing Dario Lucas , Snir Gal , Antonelli Marta C. , Bauer Reinhard
Published: June 2023

Fetal neuroinflammation and prenatal stress (PS) may contribute to lifelong neurological disabilities. Astrocytes and microglia, among the brain’s non-neuronal “glia” cell populations, play a pivotal role in neurodevelopment and predisposition to and initiation of disease throughout lifespan. One of the most common neurodevelopmental disorders manifesting between 1–4 years of age is the autism spectrum disorder (ASD). A pathological glial–neuronal interplay is thought to increase the risk for clinical manifestation of ASD in at-risk children, but the mechanisms remain poorly understood, and integrative, multi-scale models are needed. We propose a model that integrates the data across the scales of physiological organization, from genome to phenotype, and provides a foundation to explain the disparate findings on the genomic level. We hypothesize that via gene–environment interactions, fetal neuroinflammation and PS may reprogram glial immunometabolic phenotypes that impact neurodevelopment and neurobehavior. Drawing on genomic data from the recently published series of ovine and rodent glial transcriptome analyses with fetuses exposed to neuroinflammation or PS, we conducted an analysis on the Simons Foundation Autism Research Initiative (SFARI) Gene database. We confirmed 21 gene hits. Using unsupervised statistical network analysis, we then identified six clusters of probable protein–protein interactions mapping onto the immunometabolic and stress response networks and epigenetic memory. These findings support our hypothesis. We discuss the implications for ASD etiology, early detection, and novel therapeutic approaches. We conclude with delineation of the next steps to verify our model on the individual gene level in an assumption-free manner. The proposed model is of interest for the multidisciplinary community of stakeholders engaged in ASD research, the development of novel pharmacological and non-pharmacological treatments, early prevention, and detection as well as for policy makers.
Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes
Authors: Abduvokhidov Davronjon , Yusupov Maksudbek , Shahzad Aamir , Attri Pankaj , Shiratani Masaharu , Oliveira Maria C. , Razzokov Jamoliddin
Published: June 2023

The potential of cold atmospheric plasma (CAP) in biomedical applications has received significant interest, due to its ability to generate reactive oxygen and nitrogen species (RONS). Upon exposure to living cells, CAP triggers alterations in various cellular components, such as the cell membrane. However, the permeation of RONS across nitrated and oxidized membranes remains understudied. To address this gap, we conducted molecular dynamics simulations, to investigate the permeation capabilities of RONS across modified cell membranes. This computational study investigated the translocation processes of less hydrophilic and hydrophilic RONS across the phospholipid bilayer (PLB), with various degrees of oxidation and nitration, and elucidated the impact of RONS on PLB permeability. The simulation results showed that less hydrophilic species, i.e., NO, NO2, N2O4, and O3, have a higher penetration ability through nitro-oxidized PLB compared to hydrophilic RONS, i.e., HNO3, s-cis-HONO, s-trans-HONO, H2O2, HO2, and OH. In particular, nitro-oxidation of PLB, induced by, e.g., cold atmospheric plasma, has minimal impact on the penetration of free energy barriers of less hydrophilic species, while it lowers these barriers for hydrophilic RONS, thereby enhancing their translocation across nitro-oxidized PLB. This research contributes to a better understanding of the translocation abilities of RONS in the field of plasma biomedical applications and highlights the need for further analysis of their role in intracellular signaling pathways.
Heart Failure and Major Adverse Cardiovascular Events in Atrial Fibrillation Patients: A Retrospective Primary Care Cohort Study
Authors: Moltó-Balado P. , Reverté-Villarroya S. , Monclús-Arasa C. , Balado-Albiol M. , Baset-Martínez S. , Carot-Domenech J. , Clua-Espuny J.
Published: June 2023

Background: Atrial fibrillation (AF) is a common cardiac arrhythmia that is associated with an increased risk of major adverse cardiovascular events (MACE). The main goal was to analyze the links and associations between AF and MACE. Methods: A multicenter, observational, retrospective, community-based study of a cohort (n = 40,297) of the general population aged 65–95 years between 1 January 2015 and 31 December 2021 without a previous diagnosis of AF or MACE in the Primary Care setting. Results: 2574 people (6.39%) developed a first AF event, resulting in an overall incidence of 8.9/1000 people-years [CI95% 8.6–9.2]. The incidence of MACE among those with AF was 75.1/1000 people-years [CI95% 70.8–79.5], whereas among those without AF, it was 20.6/1000 people-years [CI 95% 20.2–21.1], resulting in a rate ratio of 3.65 [CI 95% 3.43–3.88, p < 0.001]. Besides, the incidence of HF with AF was 40.1 people-years [CI 95% 37.1–43.2], while in the group without AF, it was 8.3 people-years [CI 95% 7.9–8.6, p < 0.001], with a rate ratio of 4.85 [CI 95% 4.45–55.3, p < 0.001]. Before an AF diagnosis, there is already a higher risk of chronic kidney disease, ischemic cardiopathy, and peripheral artery disease. A higher risk of poor nutritional status was detected among those with MACE (49.7% vs. 26.6%, p < 0.001). Conclusions: AF diagnosis increases the incidence of heart failure fourfold. Additional information is required to establish the connection between AF, major adverse cardiovascular events, and nutritional status.
A Comprehensive Review on Monkeypox Viral Disease with Potential Diagnostics and Therapeutic Options
Authors: Rabaan Ali , Al-Shwaikh Seham , Alfouzan Wadha , Al-Bahar Ali , Garout Mohammed , Halwani Muhammad , Albayat Hawra , Almutairi Norah , Alsaeed Mohammed , Alestad Jeehan , Al-Mozaini Maha , Ashgar Tala , Alotaibi Sultan , Abuzaid Abdulmonem , Aldawood Yahya , Alsaleh Abdulmonem , Al-Afghani Hani , Altowaileb Jaffar , Alshukairi Abeer , Arteaga-Livias Kovy , Singh Kirnpal , Imran Mohd
Published: June 2023

The purpose of this review is to give an up-to-date, thorough, and timely overview of monkeypox (Mpox), a severe infectious viral disease. Furthermore, this review provides an up-to-date treatment option for Mpox. The monkeypox virus (MPXV) has remained the most virulent poxvirus for humans since the elimination of smallpox approximately 41 years ago, with distribution mainly in central and west Africa. Mpox in humans is a zoonotically transferred disease that results in symptoms like those of smallpox. It had spread throughout west and central Africa when it was first diagnosed in the Republic of Congo in 1970. Mpox has become a major threat to global health security, necessitating a quick response by virologists, veterinarians, public health professionals, doctors, and researchers to create high-efficiency diagnostic tests, vaccinations, antivirals, and other infection control techniques. The emergence of epidemics outside of Africa emphasizes the disease’s global significance. A better understanding of Mpox’s dynamic epidemiology may be attained by increased surveillance and identification of cases.
Anti-PD-1 Monoclonal Antibodies (mAbs) Are Superior to Anti-PD-L1 mAbs When Combined with Chemotherapy in First-Line Treatment for Metastatic Non-Small Cell Lung Cancer (mNSCLC): A Network Meta-Analysis
Authors: Wei Joe Q. , Yuile Alexander , Itchins Malinda , Kong Benjamin Y. , Li Bob T. , Pavlakis Nick , Chan David L. , Clarke Stephen J.
Published: June 2023

Platinum-based chemotherapy combined with anti-PD-1 or PD-L1 monoclonal antibodies (mAbs) is now standard first-line therapy for mNSCLC patients without sensitizing driver mutations. Anti-PD-1 and anti-PD-L1 mAbs are considered to be equivalent in efficacy. In the absence of head-to-head randomized control trials (RCTs), we utilized network meta-analysis (NWM) to provide an indirect comparison of their efficacy. A systematic literature review and NWM were performed using RCTs that investigated anti-PD-1 or PD-L1 mAbs ± chemotherapy in patients with mNSCLC in the first-line setting. The primary outcome was comparative overall survival (OS), while secondary outcomes were comparative progression-free survival (PFS), objective response rate (ORR), and rate of grade 3 and higher toxicities. We identified 24 RCTs. Patients treated with anti-PD-1 mAb + chemotherapy compared with anti-PD-L1 mAb + chemotherapy showed superior mOS, mPFS, and ORR with a similar rate of grade 3 and higher toxicities. This difference in mOS was most pronounced in the PD-L1 TPS 1–49% population. The two mAbs were equivalent as single agents. Anti-PD-1 mAb + chemotherapy improved mOS when compared to anti-PD-1 mAb monotherapy, whereas anti-PD-L1 mAbs + chemotherapy did not when compared to anti-PD-L1 mAb monotherapy. Head-to-head RCTs are warranted in the future.
Wnt/β-Catenin Signaling Activation Induces Differentiation in Human Limbal Epithelial Stem Cells Cultured Ex Vivo
Authors: Bisevac Jovana , Katta Kirankumar , Petrovski Goran , Moe Morten Carstens , Noer Agate
Published: June 2023

Human limbal epithelial stem cells (hLESCs) continuously replenish lost or damaged human corneal epithelial cells. The percentage of stem/progenitor cells in autologous ex vivo expanded tissue is essential for the long-term success of transplantation in patients with limbal epithelial stem cell deficiency. However, the molecular processes governing the stemness and differentiation state of hLESCs remain uncertain. Therefore, we sought to explore the impact of canonical Wnt/β-catenin signaling activation on hLESCs by treating ex vivo expanded hLESC cultures with GSK-3 inhibitor LY2090314. Real-time qRT-PCR and microarray data reveal the downregulation of stemness (TP63), progenitor (SOX9), quiescence (CEBPD), and proliferation (MKI67, PCNA) genes and the upregulation of genes for differentiation (CX43, KRT3) in treated- compared to non-treated samples. The pathway activation was shown by AXIN2 upregulation and enhanced levels of accumulated β-catenin. Immunocytochemistry and Western blot confirmed the findings for most of the above-mentioned markers. The Wnt/β-catenin signaling profile demonstrated an upregulation of WNT1, WNT3, WNT5A, WNT6, and WNT11 gene expression and a downregulation for WNT7A and DKK1 in the treated samples. No significant differences were found for WNT2, WNT16B, WIF1, and DKK2 gene expression. Overall, our results demonstrate that activation of Wnt/β-catenin signaling in ex vivo expanded hLESCs governs the cells towards differentiation and reduces proliferation and stem cell maintenance capability.
Thalamocortical Mechanisms Underlying Real and Imagined Acupuncture
Authors: Kong Qiao , Sacca Valeria , Walker Kathryn , Hodges Sierra , Kong Jian
Published: June 2023

Both acupuncture and imagery have shown potential for chronic pain management. However, the mechanisms underlying their analgesic effects remain unclear. This study aims to explore the thalamocortical mechanisms underlying acupuncture and video-guided acupuncture imagery treatment (VGAIT), a combination of acupuncture and guided imagery, using the resting-state functional connectivity (rsFC) of three thalamic subdivisions—the ventral posterolateral thalamus (VPL), mediodorsal thalamus (MD), and motor thalamus subregion (Mthal)—associated with somatosensory, limbic, and motor circuity. Twenty-seven healthy individuals participated in a within-subject randomized crossover design study. Results showed that compared to sham acupuncture, real acupuncture altered the rsFC between the thalamus and default mode network (DMN) (i.e., mPFC, PCC, and precuneus), as well as the prefrontal and somatosensory cortex (SI/SII). Compared to the VGAIT control, VGAIT demonstrated greater rsFC between the thalamus and key nodes within the interoceptive network (i.e., anterior insula, ACC, PFC, and SI/SII), as well as the motor and sensory cortices (i.e., M1, SMA, and temporal/occipital cortices). Furthermore, compared to real acupuncture, VGAIT demonstrated increased rsFC between the thalamus (VPL/MD/Mthal) and task-positive network (TPN). Further correlations between differences in rsFC and changes in the heat or pressure pain threshold were also observed. These findings suggest that both acupuncture- and VGAIT-induced analgesia are associated with thalamocortical networks. Elucidating the underlying mechanism of VGAIT and acupuncture may facilitate their development, particularly VGAIT, which may be used as a potential remote-delivered pain management approach.
HLA-DR Expression on Monocytes and Sepsis Index Are Useful in Predicting Sepsis
Authors: Quirant-Sánchez Bibiana , Plans-Galván Oriol , Lucas Ester , Argudo Eduard , Martinez-Cáceres Eva María , Arméstar Fernando
Published: June 2023

The reduction of mortality in patients with sepsis depends on the early identification and treatment of at-risk patients. The aim was to evaluate the HLA-DR expression on the surface of monocytes (MHLA-DR ratio), the sepsis index (CD64 expression on neutrophils/MHLA-DR ratio), and C-reactive protein (CRP) with the development of sepsis. We prospectively enrolled 77 critically ill patients, 59 with stroke and 18 with traumatic brain injuries. The biomarkers were tested at the baseline and 3, 6, 9, 12, and 15 days later. Most patients (71%) developed sepsis (4.2 ± 1.3 days after admission). On day 3, those subsequently developing sepsis had lower levels of MHLA-DR+ (81.7 ± 16.2% vs. 88.5 ± 12.1%, p < 0.05) and higher sepsis indexes (0.19 ± 0.19 vs. 0.08 ± 0.08, p < 0.01) than those not developing sepsis. The MHLA-DR ratio slowly recovered before day 6, while the sepsis index remained raised in septic patients up to day 9 (p < 0.05). To predict the development of sepsis, optimal cut-offs were CRP levels > 106.90 mg/mL (74.19% sensitivity, 69.49 specificity) and MHLA-DR expression rate < 72.80% (45.31% sensitivity, 89.47% specificity). The periodic monitoring of the MHLA-DR expression together with CRP and sepsis index may help to identify patients in the ICU at increased risk of developing sepsis.
The TP53 Codon 72 Arginine Polymorphism Is Found with Increased TP53 Somatic Mutations in HPV(−) and in an Increased Percentage among HPV(+) Norwegian HNSCC Patients
Authors: Moe Svein Erik , Erland Fredrik A. , Fromreide Siren , Lybak Stein , Brydoy Marianne , Dongre Harsh N. , Dhayalan Sophia M. , Costea Daniela-Elena , Vintermyr Olav K. , Aarstad Hans Jørgen
Published: June 2023

Background: Somatic TP53 mutations are frequent in head and neck squamous cell carcinoma (HNSCC) and are important pathogenic factors. Objective: To study TP53 mutations relative to the presence of human papillomavirus (HPV) in tumors in HNSCC patients. Methods: Using a custom-made next-generation sequencing (NGS) panel on formalin-fixed, paraffin-embedded tumor tissue, we analyzed somatic TP53 mutations and the TP53 single-nucleotide polymorphism (SNP) codon 72 (P72R; rs1042522) (proline → arginine) from 104 patients with HNSCC. Results: Only 2 of 44 patients with HPV-positive (HPV(+)) HNSCC had a TP53 somatic mutation, as opposed to 42/60 HPV-negative (HPV(−)) HNSCC patients (p < 0.001). Forty-five different TP53 somatic mutations were detected. Furthermore, in HPV(−) patients, we determined an 80% prevalence of somatic TP53 mutations in the TP53 R72 polymorphism cohort versus 40% in the TP53 P72 cohort (p = 0.001). A higher percentage of patients with oral cavity SCC had TP53 mutations than HPV(−) oropharyngeal (OP) SCC patients (p = 0.012). Furthermore, 39/44 HPV(+) tumor patients harbored the TP53 R72 polymorphism in contrast to 42/60 patients in the HPV(−) group (p = 0.024). Conclusions: Our observations show that TP53 R72 polymorphism is associated with a tumor being HPV(+). We also report a higher percentage of somatic TP53 mutations with R72 than P72 in HPV(−) HNSCC patients.
Challenges in Diagnosis and Therapeutic Approach of Acute on Chronic Liver Failure—A Review of Current Evidence
Authors: Marginean Cristina Maria , Pirscoveanu Denisa  , Popescu Mihaela , Vasile Corina Maria , Docea Anca Oana , Mitruț Radu , Mărginean Iulia Cristina , Iacob George Alexandru , Firu Dan Mihai , Mitruț Paul
Published: June 2023

Acute-on-chronic liver failure (ACLF) is a syndrome characterized by acute and severe decompensation of chronic liver disease (CLD) correlated with multiple organ failure, poor prognosis, and increased mortality. In 40–50% of ACLF cases, the trigger is not recognized; for many of these patients, bacterial translocation associated with systemic inflammation is thought to be the determining factor; in the other 50% of patients, sepsis, alcohol consumption, and reactivation of chronic viral hepatitis are the most frequently described trigger factors. Other conditions considered precipitating factors are less common, including acute alcoholic hepatitis, major surgery, TIPS insertion, or inadequate paracentesis without albumin substitution. Host response is likely the primary factor predicting ACLF severity and prognosis, the host immune response having a particular significance in this syndrome, together with the inflammatory cascade. The management of ACLF includes both the prevention of the precipitating factors that lead to acute liver decompensation and the support of vital functions, the prevention and management of complications, the estimation of prognosis, and the opportunity for liver transplantation.
Unveiling the Silent Danger of Childhood Obesity: Non-Invasive Biomarkers Such as Carotid Intima-Media Thickness, Arterial Stiffness Surrogate Markers, and Blood Pressure Are Useful in Detecting Early Vascular Alterations in Obese Children
Authors: Mihuta Monica Simina , Paul Corina , Borlea Andreea , Roi Cristina Mihaela , Velea-Barta Oana-Alexandra , Mozos Ioana , Stoian Dana
Published: June 2023

Obese children present a higher cardio-metabolic risk. Measuring vascular biomarkers that assess the evolution of arterial stiffness, subclinical atherosclerosis, and hypertension in such patients could be helpful in the long term. We studied 84 children, aged from 6 to 18 years: 50 obese subjects, versus 34 of normal weight. Clinical examination involved: BMI, waist circumference, waist-to-height ratio, and detection of the presence of acanthosis nigricans and irregular menstrual cycles (the latter in adolescent girls). The carotid intima-media thickness (CIMT) was measured with the Aixplorer MACH 30 echography device. The pulse wave velocity (PWV), augmentation index (AIx), and peripheral and central blood pressures (i.e., SBP, DBP, cSBP, cDBP, and cPP) were acquired through a Mobil-O-Graph device. Obese subjects underwent body composition analysis with a Tanita BC-418. Blood tests were: HOMA-IR, lipid panel, uric acid, and 25-OH vitamin D. All vascular biomarkers presented increased values in obese subjects versus controls. The following cut-off values were significant in detecting obesity: for PWV > 4.6 m/s, cSBP > 106 mmHg for the <12-year-olds, PWV > 4.5 m/s and cSBP > 115 mmHg for the 12–15-year-olds, and PWV > 5 m/s, cSBP > 123 mmHg for the >15-year-olds. AIx is higher in obese children, regardless of their insulin resistance status. Waist circumference and waist-to-height ratio correlate to all vascular parameters. HOMA-IR is an independent predictor for all vascular parameters except CIMT. Cut-off values for PWV of >4.8 m/s, SBP > 125 mmHg, and a cSBP > 117 mmHg predicted the presence of acanthosis nigricans. Obese girls with irregular menses displayed significantly higher PWV, SBP, and DPB. Elevated levels of uric acid, LDL-c, non-LDL-c, triglycerides, and transaminases, and low levels of HDL-c and 25-OH vitamin D correlated with higher arterial stiffness and CIMT values. We conclude that CIMT and the markers of arterial stiffness are useful in the early detection of vascular damage in obese children.
Recent Advances and Challenges in Textile Electrodes for Wearable Biopotential Signal Monitoring: A Comprehensive Review
Authors: Vidhya C. M. , Maithani Yogita , Singh Jitendra P.
Published: June 2023

The technology of wearable medical equipment has advanced to the point where it is now possible to monitor the electrocardiogram and electromyogram comfortably at home. The transition from wet Ag/AgCl electrodes to various types of gel-free dry electrodes has made it possible to continuously and accurately monitor the biopotential signals. Fabrics or textiles, which were once meant to protect the human body, have undergone significant development and are now employed as intelligent textile materials for healthcare monitoring. The conductive textile electrodes provide the benefit of being breathable and comfortable. In recent years, there has been a significant advancement in the fabrication of wearable conductive textile electrodes for monitoring biopotential signals. This review paper provides a comprehensive overview of the advances in wearable conductive textile electrodes for biopotential signal monitoring. The paper covers various aspects of the technology, including the electrode design, various manufacturing techniques utilised to fabricate wearable smart fabrics, and performance characteristics. The advantages and limitations of various types of textile electrodes are discussed, and key challenges and future research directions are identified. This will allow them to be used to their fullest potential for signal gathering during physical activities such as running, swimming, and other exercises while being linked into wireless portable health monitoring systems.
The Development of Soil-Based 3D-Printable Mixtures: A Mix-Design Methodology and a Case Study
Authors: Daher Jana , Kleib Joelle , Benzerzour Mahfoud , Abriak Nor-Edine , Aouad Georges
Published: June 2023

Concrete 3D printing is one of the newest technologies in the field of construction. However, despite the various opportunities that this technique offers today, it still has a high environmental impact, as most 3D-printable materials contain high amounts of cement. On the other hand, due to the large volumes of soil excavated each year across the world, there is a pressing need for proper management to dispose of it or reuse it efficiently. This study aims to develop sustainable and resistant 3D-printable materials with low environmental impact using excavated soil. Firstly, a series of tests were carried out to find the most appropriate superplasticizer and the amount required to develop the printable mixtures. Next, the extrudability and buildability were evaluated and verified to validate the printability of the developed mixtures. A 3D laboratory printer was also used to validate the printability of the mixtures on a larger scale. Then, the fresh and hardened properties of the printable mixtures were investigated. Three printable mixtures were developed, with the most environmentally friendly mixture having a soil content of 1602 kg/m3 and a cement content of 282 kg/m3. The mixtures demonstrated satisfactory characteristics and properties in both fresh and hardened states. On the one hand, the mixtures were extrudable and buildable at two laboratory scales. On the other hand, the mixtures presented sufficient compressive strengths, ranging from 16 MPa to 34 MPa, despite their high soil content and low cement content. In addition, their compressive strengths were found to be higher than the minimum strength required for structural concrete. Consequently, this study highlights the possibility of developing ecological, sustainable and resistant mixtures that can be used in 3D-printing construction applications using excavated soil.
Variability and Changes in Temperature, Precipitation and Snow in the Desaguadero-Salado-Chadileuvú-Curacó Basin, Argentina
Authors: Müller Gabriela V. , Lovino Miguel A.
Published: June 2023

We analyze the variability and changes in mean temperature, annual precipitation, and snow water equivalent (SWE) in the Desaguadero-Salado-Chadileuvú-Curacó (DSCC) basin, observed in a recent period, and their projected future values. The current climate and observed changes are studied based on the scarce observation records available in the region. Simulations from the High-Resolution Model Intercomparison Project (HighResMIP v1.0) are used for the analysis of future climate projections. The mean annual temperature presents a major rise between 1 and 2 °C throughout the region in the 1961–2020 period. The annual precipitation has nonlinear positive trends of varied importance. The SWE has decreased noticeably since 2010. Interannual variability also plays a key role, with dominant cycles between 2.8 and 4 years and between 7.5 and 10 years. The projected changes, computed as the difference between the 1985–2014 and 2021–2050 averages, reveal a rise in mean annual temperature in the entire basin, with an eastward magnitude increase from 1 to more than 2 °C. The annual precipitation is projected to increase up to 10% over most of the basin, except in the Andes, where it will decrease. The maximum annual SWE will also continue to decrease, in agreement with the trend observed in recent years.
Oil Prices, World Trade Policy Uncertainty, and the Trade Balance: The Case of Korea
Authors: Baek Jungho
Published: June 2023

This article studies the asymmetric effects that the price of crude oil has on Korean exports to and imports from its largest partners—China, the U.S., and Japan—controlling for world trade policy uncertainty. The results support the evidence of the long-run asymmetry of oil prices for Korea’s exports to Japan, and imports from China and Japan. However, there is no evidence of the short-run asymmetry of oil prices. Finally, world trade policy uncertainty appears to be more important for determining Korea’s bilateral trade in the short run than in the long run.
A FEM Structural Analysis of a Francis Turbine Blade Parametrized Using Piecewise Bernstein Polynomials
Authors: Arias-Rojas Heriberto , Rodríguez-Velázquez Miguel A. , Cerriteño-Sánchez Ángel , Domínguez-Mota Francisco J. , Galván-González Sergio R.
Published: June 2023

Several methodologies have successfully described the runner blade shape as a set of discrete sections joining the hub and shroud, defined by 3D geometrical forms of considerable complexity. This task requires an appropriate parametric approach for its accurate reconstruction. Among them, piecewise Bernstein polynomials have been used to create parametrizations of twisted runner blades by extracting some cross-sectional hydrofoil profiles from reference CAD data to be approximated by such polynomials. Using the interpolating polynomial coefficients as parameters, more profiles are generated by Lagrangian techniques. The generated profiles are then stacked along the spanwise direction of the blade via transfinite interpolation to obtain a smooth and continuous representation of the reference blade. This versatile approach makes the description of a range of different blade shapes possible within the required accuracy and, furthermore, the design of new blade shapes. However, even though it is possible to redefine new blade shapes using the aforementioned parametrization, a remaining question is whether the parametrized blades are suitable as a replacement for the currently used ones. In order to assess the mechanical feasibility of the new shapes, several stages of analysis are required. In this paper, bearing in mind the standard hydraulic test conditions of the hydrofoil test case of the Norwegian Hydropower Center, we present a structural stress–strain analysis of the reparametrization of a Francis blade, thus showing its adequate computational performance in two model tests.
A Series of New Manganese(II) Polynuclear Complexes Based on Nitrothiacalix[4]arenes: The Study of Interplay between Macrocycle Platform Flexibility and Structural Diversity of Coordination Compounds
Authors: Ovsyannikov Alexander S. , Strelnikova Iuliia V. , Shutilov Ilya D. , Islamov Daut R. , Dorovatovskii Pavel V. , Gubaidullin Aidar T. , Agarkov Artem S. , Solovieva Svetlana E. , Antipin Igor S.
Published: June 2023

Four new manganese(II) complexes, based on dinitro and tetranitrothiacalix[4]arenes, were synthesized and characterized from structural points of view in the crystalline phase. It was revealed that thiacalix[4]arenes decorated with two and four electron withdrawing groups, when combined with MnCl2, afforded the formation of similar tetranuclear complexes 1 and 2a with two non-equivalent metal ions and a rhombic geometry of the metallic cluster core. The distortion of the coordination sphere of metal cations within the obtained complexes was found to be dependent on the number of nitro groups located at the upper rim of the macrocyclic backbone, adopted in cone conformation. The tetranuclear complex 2b of a different type, displaying the formation of a dinuclear cluster core, crystallized in a non-centrosymmetric space group was obtained, when tetranitrothiacalix[4]arene, adopted in a partial cone conformation, was involved in coordination with manganese(II) cations. The switching of coordination behavior for the macrocyclic ligand in 2b was achieved due to the presence of upper-rim-disposed electron-withdrawing nitro groups, increasing the flexibility of the macrocyclic backbone by breaking the H-bonding between the OH phenolate moieties within the ligand structure. Finally, the use of 2,2′-bipyridine as an auxiliary ligand in coordination with tetranitrothiacalix[4]arene and manganese(II) cations led to the third type complex formation 3, where the macrocycle platform adopted in a 1.2-alternate conformation.
The Distribution and Diversity of Orchids
Authors: Kindlmann Pavel , Kull Tiiu , McCormick Melissa
Published: June 2023

The presence of Orchids is declining worldwide, mostly due to habitat loss and other factors like climate change, which are likely to increase in severity in the coming decades [...]
Analytical Validation of a Novel MicroRNA Panel for Risk Stratification of Cognitive Impairment
Authors: Kunwar Arzu , Ablordeppey Kenny Kwabena , Mireskandari Alidad , Sheinerman Kira , Kiefer Michael , Umansky Samuil , Kumar Gyanendra
Published: June 2023

We have been developing a novel approach to identify cognitive impairment-related biomarkers by profiling brain-enriched and inflammation-associated microRNA (miRNA) in plasma specimens of cognitively unimpaired and cognitively impaired patients. Here, we present an analytical validation of the novel miRNA panel, CogniMIR®, using two competing quantitative PCR technologies for the expression analysis of 24 target miRNAs. Total RNA from the plasma specimens was isolated using the MagMAX mirVana Kit, and RT-qPCR was performed using stem-loop-based TaqMan and LNA-based qPCR assays. Evaluation of RNA dilution series for our target 24 miRNAs, performed by two operators on two different days, demonstrated that all CogniMIR® panel miRNAs can be reliably and consistently detected by both qPCR technologies, with sample input as low as 20 copies in a qPCR reaction. Intra-run and inter-run repeatability and reproducibility analyses using RNA specimens demonstrated that both operators generated repeatable and consistent Cts, with R2 values of 0.94 to 0.99 and 0.96 to 0.97, respectively. The study results clearly indicate the suitability of miRNA profiling of plasma specimens using either of the qPCR technologies. However, the LNA-based qPCR technology appears to be more operationally friendly and better suited for a CAP/CLIA-certified clinical laboratory.
Potential Clinical Application of Organs-on-a-Chip in Periodontal Diseases: A Systematic Review of In Vitro Studies
Authors: Ardila Carlos M. , Jiménez-Arbeláez Gustavo A. , Vivares-Builes Annie Marcela
Published: June 2023

The periodontium is a unique organ from the standpoint of building an organ-on-a-chip (OoC) since it is a system that is continually threatened by microorganisms, their noxious compounds, and antigenic components. At the same time, periodontal health depends on a balanced connection between the host and the bacteria in the oral cavity, which is a complex micro-ecological environment. The objective of this systematic review of in vitro studies is to revise the potential clinical application of OoC in periodontal diseases. PRISMA was used to guide this analysis. The review framework made use of several databases, including SCOPUS, PubMed/MEDLINE, SCIELO, and LILACS as well as the gray literature. This systematic review comprised seven studies. The clinical efficacy of OoC in periodontal diseases was observed in models of the gingival crevice for the research of periodontitis, periodontal medication analysis, the interaction of multiple microbial species, pH measurements in in situ-grown biofilm, testing antimicrobial reagents, evaluation of mucosal interactions with microorganisms, and a device for quantitative exploration of microorganisms. OoC has the potential to advance our understanding of periodontal diseases by providing a more accurate representation of the oral microenvironment and enabling the development of new treatments.
Decision Making in the Restoration of Endodontically Treated Teeth: Effect of Biomimetic Dentistry Training
Authors: Kimble Paridhi , Stuhr Sandra , McDonald Neville , Venugopalan Akshaya , Campos Marcia S. , Cavalcanti Bruno
Published: June 2023

The restoration of endodontically treated teeth (ETT) is challenging as these teeth often present with structural deficiencies. Currently, there is no consensus regarding the final restoration choice. Historically, the full coverage crown was the universally selected treatment for endodontically treated teeth. With advances in adhesive and biomimetic dentistry, more minimally invasive treatment modalities have become a viable option. With this study, we aim to understand the restorative decision of the general dentist with or without additional training in biomimetic dentistry. Seventy-eight general dentists, with or without biomimetic training, were surveyed to determine their restorative preferences on five extracted posterior teeth, categorized according to volumetric loss of tooth structure, as indicated by the number of missing walls, the isthmus width, the presence or absence of marginal ridges, and cusps. CAD/CAM reconstructions were made with the teeth to analyze the volume of tooth loss and compare these with the survey results. Data were compared using the chi-squared test and Fisher’s exact test. The frequency of responses recommending a crown and the volume of tooth loss were correlated using the Pearson test (p < 0.05). For all five teeth, survey responses showed a statistically significant difference in the restorative decision of full coverage versus alternative restorations, with biomimetic dentists selecting a direct restoration or inlay/onlay in lieu of a full coverage crown (n = 63, p < 0.05). The age of the participant did not have a significant impact on the restorative decision making process for these teeth. The biomimetic trained dentists showed a greater tendency to select a crown option only when the volume of tooth loss was greatest, otherwise their restorative decisions tended towards the conservative treatment options. This study also demonstrates a novel method of digitally developing a volume of tooth loss to compare against the visual interpretation of the volume of tooth loss.