bluuebunny/crossref_metadata_2025 huggingface dataset:
Yemeni Crisis 2021 in Al-Arabiya.Net and Irna Arabic Website: A Critical Discourse Analysis Study
Authors: Rahman Farhan Muzhaffar , Abubakar Irfan
Published: August 2024

This study aims to uncover the ideological forms, tendencies, and media interests of Al Arabiya.net and the Islamic Republic News Agency (IRNA) Arabic in their coverage of the 2021 Yemen crisis. The research analyzes news text discourse using Norman Fairclough's critical discourse analysis model, which comprises three dimensions: text analysis, discourse practice, and social practice. The data for this study comes from three Al Arabiya news articles and three IRNA Arabic news articles published between July and December 2021. The research employs a qualitative descriptive method and literature review. The findings reveal that the news texts from Al Arabiya.net and IRNA Arabic exhibit distinct ideological forms and biases. Regarding the 2021 Yemen crisis, the Al Arabiya.net texts demonstrate a negative bias towards the Houthi group while supporting the Yemeni government led by Abdrabbuh Mansur Hadi. Conversely, the IRNA Arabic texts show a negative bias towards the United States, Israel, and the Saudi-led Arab coalition, which has been militarily involved in the Yemen conflict since 2015. The IRNA Arabic texts display sympathy towards the Houthi group and argue that the Arab coalition's actions in the Yemen conflict have worsened the crisis.
Beating Barriers to Formative Assessment in a Testing-Oriented Nation
Authors: Defianty Maya , Wilson Kate
Published: August 2024

AbstractIndonesia has a been a testing-oriented country since its inception. Yet, over the past decade, assessment policies in Indonesia have gradually strengthened their emphasis on formative assessment. In general, Indonesian teachers’ understanding of formative assessment is still relatively modest; in fact, some teachers still consider formative assessment to be challenging, if not impossible.  Nevertheless, others have embraced the concept and have managed to overcome the barriers of large classes, limited time, and the long-standing culture of high-stakes testing.  This article aims to provide examples of good practice in formative assessment in ELT.  We present the results of a multiple case study, involving eight ELT senior high school teachers representing various learning contexts in Indonesia. Drawing on data from interviews and classroom observations, the study revealed that these teachers recognized the barriers in implementing formative assessment deriving from limited time and large classes, yet they were able to overcome these barriers by using teaching modifications and technological tools as solutions to applying formative assessment. This study highlights the pivotal role of teachers’ assessment literacy to enhance and reap the benefits from formative assessment. AbstrakIndonesia telah menjadi negara yang berorientasi pada ujian sejak awal berdirinya. Tetapi, selama dekade terakhir, kebijakan penilaian di Indonesia secara bertahap semakin menekankan pada penilaian formatif. Secara umum, pemahaman guru-guru Indonesia tentang penilaian formatif masih relatif terbatas; faktanya, beberapa guru masih menganggap penilaian formatif sebagai hal yang menantang, bahkan tidak mungkin dilakukan. Meskipun demikian, beberapa guru telah menerima konsep ini dan berhasil mengatasi hambatan seperti kelas yang besar, waktu yang terbatas, dan budaya ujian berisiko tinggi yang telah berlangsung lama. Artikel ini bertujuan untuk memberikan contoh praktik baik dalam penilaian formatif dalam pengajaran Bahasa Inggris. Kami menyajikan hasil studi multi-kasus, yang melibatkan delapan guru Bahasa Inggris sekolah menengah atas yang mewakili berbagai konteks pembelajaran di Indonesia. Berdasarkan data dari wawancara dan observasi kelas, studi ini mengungkapkan bahwa para guru ini menyadari hambatan dalam menerapkan penilaian formatif yang berasal dari waktu yang terbatas dan kelas yang besar, namun mereka mampu mengatasi hambatan tersebut dengan menggunakan modifikasi pengajaran dan alat teknologi sebagai solusi untuk menerapkan penilaian formatif. Studi ini menyoroti peran penting literasi penilaian guru untuk meningkatkan dan memetik manfaat dari penilaian formatif. How to Cite: Defianty, D., Wilson, K. (2024). Beating Barriers to Formative Assessment in a Testing-Oriented Nation. TARBIYA: Journal of Education in Muslim Society, 11(1), 1-12. doi:10.15408/tjems.v11i1. 40391.
University Students' Awareness and Preparedness for Natural Disasters: A Study on Preventing Landslides, Earthquakes, and Volcanic Eruptions in Indonesia
Authors: Yoshinori Fujikawa , Tuswadi Tuswadi , Takehiro Hayashi , Tetsuo Isozaki , Subekti Niken
Published: August 2024

AbstractThis study seeks to understand the disaster-prevention consciousness focused on landslide, earthquake, and volcanic eruption among 22 students pursuing a major in Geography in the Social Science Faculty, Semarang State University (UNNES), Indonesia. It analyzed the students’ consciousness through short comments toward questions “What can we do?”, which focused on preventing, defending, reducing, and mitigating the impacts of the three kinds of natural disasters. The average number of the description was 5.7, and the median was 5. More than half of the students mentioned points related to “before disaster and usual life,” “after disaster and restoration,” “hardware/structural,” and “software/non-structural.” All the answers described ideas with reasons. But only four students described ideas based on educational viewpoints, indicating students’ lack of scientific viewpoints in elaborating their ideas about disaster prevention. This finding calls for improving students’ consciousness regarding disaster prevention through education.AbstrakPenelitian ini bertujuan untuk memahami kesadaran pencegahan bencana yang difokuskan pada tanah longsor, gempa bumi, dan letusan gunung berapi di antara 22 mahasiswa yang mengambil jurusan Geografi di Fakultas Ilmu Sosial, Universitas Negeri Semarang (UNNES), Indonesia. Kesadaran mahasiswa dianalisis melalui komentar singkat terhadap pertanyaan "Apa yang bisa kita lakukan?", yang difokuskan pada pencegahan, pertahanan, pengurangan, dan mitigasi dampak dari tiga jenis bencana alam tersebut. Rata-rata jumlah deskripsi adalah 5,7, dan median adalah 5. Lebih dari setengah mahasiswa menyebutkan poin-poin yang terkait dengan "sebelum bencana dan kehidupan biasa," "setelah bencana dan pemulihan," "perangkat keras/struktural," dan "perangkat lunak/non-struktural." Semua jawaban menggambarkan ide-ide dengan alasan yang mendasarinya. Namun, hanya empat mahasiswa yang menguraikan ide-ide berdasarkan sudut pandang pendidikan, yang menunjukkan kurangnya sudut pandang ilmiah dalam mengelaborasi ide-ide mereka tentang pencegahan bencana. Temuan ini menunjukkan perlunya peningkatan kesadaran mahasiswa mengenai pencegahan bencana melalui pendidikan.  How to Cite: Yoshinori, F, Tuswadi, Takehiro, H, Tetsuo, I., Subekti, N. (2024). "University Students' Awareness and Preparedness for Natural Disasters: A Study on Preventing Landslides, Earthquakes, and Volcanic Eruptions in Indonesia. TARBIYA: Journal of Education in Muslim Society, 11(1), 81-92. doi:10.15408/tjems.v11i1.40444.
Barboza, J. O náufrago da existência
Authors: Picoli Gleisy
Published: August 2024

O livro O náufrago da existência: Machado de Assis e Arthur Schopenhauer – Caricatura, paródia, tragédia e ética animal, de Jair Barboza, explora a influência de Schopenhauer na obra de Machado de Assis, propondo que a personagem Quincas Borba seja uma caricatura do filósofo alemão e sua filosofia do humanitismo, uma paródia do pessimismo metafísico schopenhaueriano. Barboza baseia-se em descobertas bibliográficas e analisa obras como Memórias póstumas de Brás Cubas e Quincas Borba para fundamentar sua tese. Ele também destaca a ética animal em Machado, influenciada por Schopenhauer, e como essa abordagem foi seguida por outros autores brasileiros. O livro é destinado a leitores interessados na intersecção entre literatura e filosofia.
Semantic shifts of ‘coming out’ and ‘come out of the closet’
Authors: Witalisz Alicja
Published: August 2024

Juxtaposing lexicographic data with authentic corpora-excerpted contexts, this article investigates the semantic variance of coming out and come out of the closet in English and Polish. It discovers the linguistic sources of the LGBTQ+-related sense of coming out in English and focuses on the semantic shift from its sociolectal LGBTQ+-related usage towards nonsociolectal pragmatically marked uses, which result in extended collocability (e.g. Eng. coming out as an atheist; Pol. ateistyczny coming-out). The study contrasts English and Polish corpus data to see whether the nonsociolectal usage of Polish coming out can be argued to be contact-induced – that is, modelled on English. Language contact research shows that contact-induced innovations undergo semantic change through conscious and creative exploitation by recipient language speakers to realise their pragmatic needs. The cognitive-onomasiological theoretical framework, coupled with a usage-based methodology, allows investigation of how native and contact-induced phenomena are used by native and recipient language speakers.
The Limits of Discursive Interpretation: A Translation of Kitāb iʿjāz al-bayān fī tafsīr Umm al-Kitāb by Ṣadr al-Dīn Qūnawī
Authors: Shaker Anthony F.
Published: August 2024

Ṣadr al-Dīn Qūnawī (d. 1274) is arguably the most important thinker of the generation following the main founders of medieval philosophy—al-Fārābī, Ibn Sīnā, Ibn ʿArabī and Suhravardī—and before Mullā Ṣadrā. Yet, almost nothing of his writings has been translated into English. In this influential work he independently explores speech (divine and human) as the unfolding relationality of knowing and being. This is the first annotated translation of his magnum opus The Limits of Discursive Interpretation. The Translator’s introduction and notes shed a detailed light on the linguistic sources of Qūnawī’s lexicon. The Introduction also summarizes the key ideas of the book and explains their significance to philosophy. In Part One Qūnawī begins by arguing that the failure of theoretical proofs to establish the reality of a thing does not itself disprove that reality. He elucidates the canons of thinking in relation to ‘tasting’ (experience) and the question of the ‘realities of things’ where knowing and being unfold dynamically from their ‘root’ in divine hiddenness and manifestation. He goes on to detail the concepts and the rules of relational subordination that govern these realities according to rootedness and mutual distinctions. Many of these tools are derived from linguistics, which the translator brings to bear on Qūnawī ‘s work for the first time. They enable him to transform the narrow sense in which Ibn Sīnā declared man incapable of grasping the realities of things. In the end, according to Qūnawī, without a proper understanding of rootedness as the source of the realities’ mutual distinctions, thinking remains relational, unequal to the thinking subject’s goal of self-realization and incapable of fully rendering the real (not to be confused with empirical facticity) without folding back on itself. In Part Two he details the semiology by which, not only the contents of the Qur’ān but, primarily the ontological dimensions of God’s speech are disclosed as the veiling and unveiling, exteriorization and interiorization of being.
On the use of a confuser to improve the accuracy of an ultrasonic flow meter
Authors: Kubenin Aleksandr , Gagarin Vladimir , Pavlenko Natalya , Alekseev Dmitry
Published: August 2024

Unlike conventional gas meters, ultrasonic flow meters have advantages such as non-contact operation, minimal pressure loss and high accuracy. The use of integration methods in the theory of ultrasonic flow meters also imposes its own requirements on the profiles of the gas flow velocity passing through the flow meter. To level the flow profiles, devices such as flow straighteners in the form of perforated disks or disks with guide blades, as well as changes in the geometric shape of the flow part of the flowmeter, can be used. The work analyzes the influence of the narrowing of the flow part of a two-beam ultrasonic flow meter on the accuracy of measurements. The influence of the tapering part of the flow meter on the accuracy of its readings is shown. The article compares integration errors in a flow meter for different diameters of the flow path, as well as for different numbers of flow meter beams at different Reynolds numbers. It has been demonstrated that three-beam and four-beam integration schemes are more accurate than the two-beam scheme. The flow velocity profiles in the flow meter were compared. It has been shown that narrowing the flow part of the flow meter improves the measurement accuracy by reducing the integration error. The effect of changing the shape of the flow profile as a result of narrowing the flow part of the ultrasonic filter is estimated at 0,1–0,3 % over the entire operating range of the flow meter. At the same time, a zone was identified in the region of the laminar-turbulent transition, in which the narrowing of the flow part of the flow meter has a negative impact on the measurement accuracy. The critical speeds corresponding to this area are indicated. It is shown that the number of rays does not affect the existence of the identified region.
Representation of one's own birth: problematics and gender differences
Authors: Naumchuk Nadezhda
Published: August 2024

The article examines the concept of mental representation. A theoretical contrast of its concepts is presented. The features of the formation of the representation of the self are considered. It is reported that the ability to represent is present from infancy, however, differentiation of oneself from objects is formed by the age of 3. The author introduces the problem of representing one’s own birth as a mental reflection of the external situation of appearance in the family and studies it within the framework of a dissertation research. There were empirically studied «negative», «neutral» and «positive» representations of one’s own birth using a sample of 189 participants. The sample was divided based on the results of the answer to the question «Do you think that your parents/mother did not want you to be born?». Four groups were identified: 1. «They wanted an abortion», 2. «They took it normally», 3. «Happy to be pregnant», 4. «Child of a different sex». There was used a nonparametric Mann-Whitney U test to calculate differences between groups. The author presents differences in the severity of representations between women and men. It was found that the «negative» representation of one’s own birth is expressed to a greater extent in women than in men. It was shown that significant differences between the representation groups concerned the presence of children. The differences were due not only to groups, but also to sexual differentiation. The results of the work confirm the need for separate research into the indicators of men and women in the further development of the problem. The findings explore participants' own feelings of worthlessness rather than their mother's perceptions of them. The research materials may be useful for individual psychotherapeutic practice with adults and children, as well as in the methodological and empirical scientific field.
América Latina: desenvolvimento, dependência e o papel do capital chinês*
Authors: Ribeiro Valéria Lopes
Published: August 2024

Resumo Nas últimas décadas o capitalismo passou por diversas transformações na esfera produtiva e tecnológica, sejam essas relacionadas às novas tecnologias em áreas de comunicação e informação, ou à infraestrutura dos sistemas de informação, inovações na inteligência artificial, empresas digitais e de plataforma. Todas essas mudanças vêm moldando a forma pela qual a acumulação de capital opera e também a relação entre os países dentro da hierarquia interestatal. Partindo de elaborações teóricas clássicas e atuais sobre a dependência latino-americana - e com foco nas transformações produtivas e tecnológicas -, este trabalho visa a investigar como a dependência do continente se apresenta atualmente diante desta nova conjuntura e quais suas diferentes formas de manifestação. Os resultados do trabalho apontam para o quadro em que, enquanto países centrais, e alguns emergentes como a China, avançam na fronteira destas novas tecnologias, outros, como os latino-americanos, permanecem na mesma posição histórica de fornecedores de matérias-primas e também na posição mais recente de consumidores destas novas tecnologias. A partir das elaborações teóricas sobre a dependência e da investigação das transformações em curso, busca-se demostrar como tais transformações relacionam-se ao desenvolvimento histórico do capitalismo em que novos atores (como a China), ao adentrar cada vez mais na fronteira do desenvolvimento tecnológico e aproximar-se dos países latino-americanos, assumem uma posição relevante na definição do quadro da dependência do continente.
The Overexpression of EP4 Attenuates the Killing Ability of CD8+ T Cells against Prostate Cancer Cells through the PI3K/AKT Signaling Pathway
Authors: Zhou Ying , Zou Lanying , Xu Jun , Zhou Xiaoping , Zhao Huichuan
Published: August 2024

Immunotherapy has shown significant promise in the clinical management of prostate cancer (PCa), and prostaglandin E receptor 4 (EP4) is a key governing factor in PCa progression. However, the molecular mechanisms by which EP4 influences immunotherapy in PCa have yet to be elucidated. This investigation was designed to unravel the specific mechanisms through which EP4 affects the killing ability of CD8+ T cells against PCa cells. Immunohistochemistry was utilized to assay the expression of EP4, programmed death ligand 1 (PD-L1), and CD8+ T cell infiltration in tissue samples, and quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB) were used to evaluate EP4 expression in cells. PCa cell lines with either EP4 knockdown or overexpression were co-cultured with CD8+ T cells. Lactase dehydrogenase toxicity assays were employed to measure CD8+T cell killing ability, and ELISA was employed to measure interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), and interleukin-2 (IL-2) levels. Expression of T cell exhaustion markers was detected by flow cytometry. Rescue experiments were conducted utilizing 3-methyladenine [a phosphoinositide 3-kinase (PI3K) inhibitor] and PD-L1 knockdown. The impact of EP4 overexpression on the PI3K/AKT signaling pathway-mediated PD-L1 expression and its subsequent modulation of CD8+ T cell killing ability against PCa cells was assessed through qRT-PCR, WB, flow cytometry, and immunofluorescence. EP4 exhibited a substantial upregulation in both PCa tissues and cells, displaying a positive correlation with PD-L1 expression and a converse negative correlation with the infiltration of CD8+ T cells. Knockdown of EP4 expression inhibited CD8+ T cell exhaustion, enhanced CD8+ T cell killing ability against PCa cells, increased the levels of IFN-γ, IL-2, and TNF-α, and decreased PD-L1 expression. Conversely, EP4 overexpression resulted in opposite effects, but treatment with 3-methyladenine mitigated EP4-induced promotion of PD-L1, p-AKT, and t-AKT expression. Furthermore, the knockdown of PD-L1 mitigated the inhibitory impact of EP4 overexpression on the killing ability of CD8+ T cell and the levels of IFN-γ, IL-2, and TNF-α, all while leaving the expression of p-AKT and t-AKT unaffected. EP4 was significantly overexpressed in PCa and activated PD-L1 expression via the PI3K/AKT signaling pathway, thereby suppressing the activity of CD8+ T cells.
Risk management of engineering projects installation using Analytic Hierarchy Process
Authors: Wu Hanmei , Cai Xiaoqing , Feng Man
Published: August 2024

The electromechanical installation project in the city park is confronted with numerous risks, prominently involving uncertainty and human factors, which are critical to the project’s overall success. This study carefully identified and categorized the project risks into a detailed hierarchical structure, employing the Analytic Hierarchy Process (AHP) to systematically quantify their relative importance. The findings revealed that uncertainty factors bear a significantly greater influence on the overall project risks compared to human factors. Notably, the research highlighted an inverse relationship between the reduction of uncertainty and the effectiveness of human intervention, thus offering a novel perspective on managing project risks. This study's technical innovation lies in the creation of a comprehensive risk assessment framework, which enables project managers to accurately identify, evaluate, and devise effective control strategies tailored to the specific risks identified. Future research endeavors will focus on harnessing advanced data analysis technologies and artificial intelligence algorithms to enhance the risk assessment process further, aiming to improve decision-making accuracy, optimize resource allocation, and ultimately boost project success rates.
Aging Faces, Voice, and Barbara Steele’s Revenge
Authors: Gills Libertad
Published: August 2024

"Aging Faces, Voice, and Barbara Steele’s Revenge" begins with fragments from La maschera del demonio (Mario Bava, 1960), an Italian gothic horror film that is essentially about the process of aging as an evil in itself. In this film the English actress Barbara Steele, who would later go on to star in several similar films of the period, plays two women: one young and one old/dead, the former good and the latter evil. The film uses makeup and changes of speed to show the horrific (as well as spectacular) aging process. The video essay then brings in films made by Steele later in her life (including Federico Fellini’s 8½ from 1963, David Cronenberg’s Shivers from 1975, and Gionata Zarantonello’s The Butterfly Room from 2012 in which she plays a murderous elderly woman), allowing the viewer to see a more realistic (?) aging process. At only 40 years old, she speaks to Delphine Seyrig in Sois belle et tais-toi (1981) about her acting career already in the past tense, critical of the films she’s made in a male-dominated industry and how she feels reduced by someone else’s image of her.
Fragmentación institucional y consolidación de la paz de Naciones Unidas 2017-2021
Authors: Oviedo Hernández Edicson Andrés
Published: August 2024

Objetivo: el artículo analizó el funcionamiento de la estructura organizativa de la consolidación de la paz de Naciones Unidas durante el período 2017-2021. Esta no solo constituyó un área de operaciones de dicha organización, sino que expresó la existencia de un proyecto global de pacificación posconflicto que surge en el marco de la identificación de la paz como un bien público global. Método: a nivel metodológico se propuso un método exploratorio con base en análisis documental, recurriendo a las resoluciones, actas de reunión, informes y evaluaciones producidos por entidades directamente relacionadas con la consolidación de la paz. Resultados: entre los hallazgos se destacó que existen interrupciones de información relacionadas con falencias institucionales y alta concentración de los donantes, debido tanto a la ausencia de un mecanismo estable para la obtención de recursos como a la susceptibilidad de la organización a fluctuaciones externas. Conclusión: se concluyó que el panorama de la consolidación de la paz es crítico, pues no existe un camino claro a través del cual garantizar la mayor participación de otras áreas de la organización, de donantes alternativos o de actores capaces de contribuir a su arraigo institucional.
La migración venezolana en Colombia: desafíos y oportunidades en un contexto de crisis
Authors: Guerrero Arroyave Claudia Patricia , Pedreros Norato Andrea , Quiroz Monroy Liliana Margarita
Published: August 2024

Resumen: Este artículo presenta los hallazgos de un estudio cualitativo que tenía como objetivo identificar los desafíos y oportunidades explorando las experiencias de los migrantes venezolanos en Colombia y las respuestas de las comunidades de acogida. Utilizando un diseño metodológico desde un paradigma interpretativo, se recolectaron datos a través de análisis documental, entrevistas en profundidad, grupos focales y observación participante. El análisis de datos se realizó utilizando el enfoque de teoría fundamentada, identificando patrones, categorías y temas emergentes. Los resultados destacan la importancia del Permiso Especial de Permanencia (PEP) como medida gubernamental para regularizar la situación migratoria de los venezolanos. Además, se resalta el papel crucial de la sociedad civil en la prestación de ayuda humanitaria y servicios de apoyo. En conclusión, se evidencia la necesidad de colaboración entre el gobierno y la sociedad civil para abordar la crisis migratoria venezolana en Colombia, subrayando la importancia de políticas y prácticas más efectivas y humanas para la integración de los migrantes.
Das Noch-nicht-Bewusste. Protentionales Bewusstsein und die Entstehung des Neuen
Authors: Fuchs Thomas
Published: August 2024

Not only our conscious expectations, wishes and intentions are directed towards the future, but also pre- or unconscious tendencies, hunches and anticipations. Using a term coined by Ernst Bloch, they can be summarized as the not-yet-conscious. This not-yet-consciousness usually unfolds spontaneously and without a plan; it is not expected or striven for, but rather emerges in consciousness in such a way that the subject is surprised by itself, so to speak. This gives rise to phenomena such as the conspicuous, the accidental, the new and the improvised, which are particularly important for understanding creative and therapeutic processes. The aim of this article is to develop a general phenomenological framework for understanding not-yet-conscious dynamics and their unfolding in different contexts. To this end, the structure of the not-yet-conscious is examined in the light of protention, in which the phenomenon is fundamentally located.
Zwischen Einschlafen und Aufwachen
Authors: Wahie Sarvesh
Published: August 2024

The issue with the phenomenon of sleep is that while asleep, I cannot say that I am sleeping. Only upon waking do statements emerge that indicate that sleep has already occurred. Similarly, while lying in bed awaiting sleep, statements can even be made prior to falling asleep, suggesting that sleep will take place subsequently. For these two reasons, the following thesis can be formulated: Sleeping is a phenomenon that occurs between falling asleep and waking up. Accordingly, the objective of this paper is to describe this interstice and thereby to examine the lightless state of sleeping consciousness.
Rethinking Borderline Cases of Personal Identity: A First-Person Perspective
Authors: Dutta Shewli
Published: August 2024

Personal identity include both first-person and third-person identities. The objective of the essay is to demonstrate the primitiveness of first-person identity, or self-identity, by re-examining a few well-known thought experiments that are referred to as ‘borderline cases of personal identity’. The primary goal of the essay is to demonstrate that no borderline case poses a significant challenge to first-person identity. The discussion is divided into three sections. The first section motivates the debate. Additionally, the importance of the first-person identity statement ‘I am I’, which is immune from error through misidentification, is established. In the second section, five well-known thought experiments are evaluated to demonstrate that none of them poses a genuine threat to first-person identity. In the third and final part, some real pathological case studies are analysed to strengthen the main argument.
Immunofluorescence of GM2 v1
Authors: Park Hankum , V Hundley Frances
Published: August 2024

Selective purification of early endosomes can be achieved through affinity capture of the early endosome-associated protein EEA1 (termed Endo-IP) (Park et al. 2022). These purified endosomes can be used for proteomic and lipidomic studies to obtain snapshots of early endosomes. Here, we present an immunofluorescence protocol to assess the extent of colocalization between FLAG-EEA1 and RAB5 with and without the Dynamin-1 and -2 (DNM1/2) inhibitor Dyngo4a.
Bakteriel WGS - SSI v1
Authors: Hass THTR Thorsen Theis , SB Sekventeringsenheden
Published: August 2024

Method used at Sequencing Core Facility - Statens Serum Institut for sequencing of bacterial isolates. This protocol is specified for high-throughput sequencing of 96 isolates (sometimes less depending on the number of isolates with large genomes). Furthermore, the genomes are sequenced on the Illumina sequencing platform using a MiSeq and the MiSeq 500-cycle v2 kit. Note, this protocol is in Danish. Note, it is also possible to use a 300-cycle Mid Output Kit v2.5 on a NextSeq for the sequencing, but this will not be covered in this protocol.
High-Capacity cDNA Reverse Transcription v1
Authors: Martell Martinez Hector
Published: August 2024

This protocol details the high-capacity cDNA reverse transcription.
Pre-Imaging Liquid Growth Medium - Yeast v1
Authors: Hammer Mathias , Rossouw Ammeret , Lari Azra , Montpetit Ben , Grunwald David
Published: August 2024

This protocol describes the steps to prepare liquid culture medium for Saccharomyces cerevisiae. This liquid medium is used to optimize yeast cultures for fluorescence imaging by the reduction of auto-fluorescence through an abundance of Adenine [1].
Imaging of cholinergic interneurons in post-mortem rodent tissue to identify striatal satellite astrocytes v1
Authors: Raina Shinil , J Cragg Stephanie
Published: August 2024

This protocol details confocal image acquisition steps and the analysis pipeline used to calculate inter-soma distance between cholinergic interneurons/pMSNs and their nearest astrocyte. The partner Protocol: Immunofluorescent Labelling of Post-Mortem Rodent Brain Tissue describes how to label cholinergic interneurons/pMSNs along with striatal astrocytes in PFA perfusion-fixed, 50-µm thick, post-mortem rodent brain tissue.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices while optogenetically activating astrocytes v1
Authors: Raina Shinil , M Roberts Bradley , J Cragg Stephanie
Published: August 2024

This protocol is to assess whether optogenetically activating astrocytes affects the dopamine concentration released following electrical stimulation.
An Exploration of the Role of Socioeconomic and Cultural Influences on Weight Management in Black Women
Authors:   , Eaton Tiffany , Kumanyika Shiriki K.
Published: August 2024

Obesity is a widely studied area in public health, with numerous, varied public health efforts and multiple research areas to study; however, progress in addressing the epidemic is hampered by increasing prevalence nationwide and the presence of racial/ethnic, gender and socioeconomic disparities. This calls for a need to critically examine and evaluate approaches that have been or are currently being used to address this complex public health issue - particularly in Black women. The purpose of this three-part dissertation was to explore how race, socioeconomic status (SES) and culture influence the ways in which socioeconomically diverse Black women in the United States approach weight management. The first study was a scoping review of 72 articles to examine what is known about how race, SES and culture influence weight loss outcomes for Black women enrolled in behavioral weight loss programs. Findings indicate that there are a variety of socioeconomic and cultural factors that influence weight management behaviors, and the ways in which they can positively or negatively influence weight loss outcomes for Black women across SES. The second study, a qualitative inquiry, explored the experience of weight management among 20 Black women of different SES who either lived and/or worked in Philadelphia County, Pennsylvania. Findings from this study identified five emergent themes related to weight management: (1) cultural nuances; (2) "it [COVID] just shifted the norm"; (3) the influence of social media; (4) acknowledgement of privilege; and (5) the role of work culture. Lastly, the third study involved applying constructs of the Getting to Equity in Obesity Prevention (GTE) Framework to a subsample of articles (n=35) from the first study to assess where and how equity-related considerations were applied to behavioral weight loss interventions for socioeconomically diverse Black women. The findings from this study led to an adaptation of the GTE framework that includes equity-related considerations that can be useful for researchers and practitioners in designing and implementing individually-oriented behavioral weight loss interventions. This dissertation research contributes to the body of research a better understanding of the landscape of weight loss literature as it pertains to Black women, socioeconomic status, and cultural influences. In addition, these three studies inform recommendations that can inform future obesity-related research and praxis in an effort to improve reporting of weight loss studies, suggestions for potentially maximizing the effectiveness of weight loss efforts for Black women and ways to address root causes of obesity disparities.
Hubungan Self-Esteem dan Impulsive Buying pada Produk Skincare Pengikut Akun Mention Confession di Twitter
Authors: Michelle Thalia Roteng  , Yudi Tri Harsono
Published: August 2024

With the development of beauty standards in Indonesia, the popularity of skincare and its purchasing interest has also increased. Technological advancements have made it easier to share and receive information related to skincare products, which influences their purchasing decisions, such as through the mention confession account on Twitter. There are various types of mentions confession account created for different discussion topics and information, the one that relevant with skincare products is @ohmybeautybank. This study aimed to explore the relationship between self-esteem and impulsive buying of skincare products among followers of the mention confession account on Twitter. Data was collected using questionnaire using incidental sampling with the following criteria: 1) followers of the @ohmybeautybank Twitter account, and 2) buyers and users of skincare products. A total of 201 respondents obtained. Data were analyzed using Spearman Rank correlation with SPSS Statistics version 25 for Windows. The results showed that 1) there was a significant negative correlation between the self-liking dimension and impulsive buying of skincare products among followers of the @ohmybeautybank account on Twitter, and 2) there was a significant negative correlation between the self-competence dimension and impulsive buying of skincare products among followers of the @ohmybeautybank account on Twitter.
AbstrakSeiring dengan berkembangnya standar kecantikan di Indonesia, popularitas skincare dan minat pembeliannya juga semakin meningkat. Perkembangan teknologi kemudian memberikan kemudahan bagi individu dalam menyebarkan dan menerima informasi terkait produk skincare yang kemudian berpengaruh dalam pembelian, salah satunya melalui akun mention confession di Twitter. Terdapat beragam jenis mention confession yang dibuat untuk topik diskusi dan informasi yang berbeda-beda, terkait produk skincare akun yang berkaitan adalah akun @ohmybeautybank. Penelitian ini bertujuan untuk melihat hubungan antara self-esteem dan impulsive buying pada produk skincare pengikut akun mention confession di Twitter. Data dikumpulkan menggunakan kuesioner dengan teknik incidental sampling berdasarkan pada kriteria berikut, 1) pengikut akun Twitter @ohmybeautybank, dan 2) pembeli dan pengguna produk skincare. Dari penyebaran kuesioner didapatkan sebanyak 201 responden. Data dianalisis menggunakan teknik korelasi spearman rank dengan bantuan SPSS Statistics versi 25 for windows. Hasil penelitian menunjukkan bahwa, 1) terdapat hubungan negatif yang signifikan antara dimensi self-liking dan impulsive buying pada produk skincare pengikut akun mention confession @ohmybeautybank di Twitter, dan 2) terdapat hubungan negatif signifikan antara dimensi self-competence dan impulsive buying pada produk skincare pengikut akun mention confession @ohmybeautybank di Twitter.
Glass Child dalam Kerangka Teori Locus of Control
Authors: Moza A. Fadilah  , Nauroh N.A.T. Elsifa  , Ragil W. Hernawati  , Ulfa Masfufah
Published: August 2024

Glass child, is a child who lives and grows up with siblings with disabilities or chronic illnesses. Glass child behavior is influenced by external and internal factors. External factors come from stimulus from outside that encourages the glass child to respond, while internal factors include the perceptions and emotional turmoil that the glass child is feeling. The aim of this research is to examine the glass child phenomenon through the locus of control theory by Rotter, by analyzing the dynamics of behavior in glass children. The method used in this research is a literature study. Through the analysis of eight journals, it can be seen that the locus of control in glass children tends to lead to an external locus of control. Namely, the glass child believes that as an individual has no control over her or his behavior, but is controlled by things outside himself such as other people, fate, in this case, such as parents, and other family members, who have high expectations, as well as environmental perceptions. Belief in fate, namely having a sibling with a disability. In addition, individuals with an extremely external locus of control tend to be more easily stressed and experience depression.
AbstrakGlass child, anak yang tinggal dan tumbuh bersama dengan saudara dengan disabilitas maupun penyakit kronis. Perilaku glass child dipengaruhi oleh faktor eksternal dan faktor internal. Faktor eksternal yaitu ketika ada stimulus dari luar yang mendorong glass child untuk memberikan respon, sedangkan faktor internal mencakup persepsi dan gejolak emosi yang dirasakan glass child selama tumbuh dengan saudara disabilitas dan memiliki penyakit kronis. Tujuan dari penelitian ini adalah menelaah fenomena glass child melalui teori locus of control oleh Julian B. Rotter, dengan menganalisis dinamika perilaku pada glass child. Metode yang digunakan dalam penelitian ini adalah studi literatur. Melalui analisis delapan jurnal dan artikel dapat diketahui bahwa locus of control pada glass child cenderung mengarah ke locus of control eksternal. Yaitu, bahwa glass chlid percaya bahwa sebagai individu ia tidak memiliki kendali atas perilakunya, melainkan dikendalikan oleh hal hal diluar dirinya seperti orang lain, dan nasib. Orang lain, dalam hal ini seperti orang tua, dan anggota keluarga yang lain, yang berekspektasi yang tinggi, serta persepsi lingkungan terhadap saudara. Kepercayaan terhadap nasib yaitu nasib memiliki saudara kandung dengan disabilitas sebagai alasan yang akan menentukan diri dan perilakunya dimasa depan. Selain itu, individu dengan locus of control eksternal yang ekstrim cenderung lebih mudah stress.
Perilaku Konsumtif dalam Komunitas Game Genshin Impact
Authors: Arva Itqiya Dahlan  , Rakhmaditya Dewi Noorrizki
Published: August 2024

Online games are increasingly popular among people, especially teenagers and adults, because of the various challenges they provide. Some online games can be played for free but have a top-up feature so players can buy characters or weapons in the game. This can then lead to a tendency towards consumer behavior in online game players, one of the online games being played is Genshin Impact. This research aims to find out the description of consumer behavior carried out in the Genshin Impact community and how the influence of this community on individuals carrying out consumptive behavior is described. This research uses quantitative research methods with a descriptive research design. Data was collected via Google form which was filled in by 19 respondents. The results of this study showed that participants did not engage in consumptive behavior. However, only spend money on top-ups to get the weapons or characters they want and don't waste money. Likewise, it was found that the Genshin Impact game community did not have a big influence on respondents to carry out consumptive behavior.
AbstrakGame online semakin digemari oleh kalangan masyarakat, khususnya remaja hingga dewasa karena beragam tantangan yang diberikan didalamnya. Beberapa game online dapat dimainkan gratis namun memiliki fitur top-up agar para pemain bisa membeli karakter atau senjata yang berada di dalam game tersebut. Hal ini kemudian dapat menimbulkan kecenderungan perilaku konsumtif pada pemain game online, salah satu game online yang dimainkan ialah Genshin Impact. Tujuan dilakukannya penelitian ini untuk mengetahui gambaran perilaku konsumtif yang dilakukan dalam komunitas Genshin Impact dan bagaimana gambaran pengaruh komunitas tersebut untuk individu melakukan perilaku konsumtif. Penelitian ini menggunakan metode penelitian kuantitatif dengan rancangan penelitian deskriptif. Data dikumpulkan melalui google form yang diisi oleh 19 responden. Hasil dari penelitian ini menunjukkan bahwa partisipan didapatkan tidak melakukan perilaku konsumtif. Namun, hanya mengeluarkan uang untuk top-up demi mendapatkan senjata atau karakter yang mereka inginkan dan tidak sampai ke tahap pemborosan. Begitu pula komunitas game Genshin Impact didapatkan tidak memberikan pengaruh besar pada responden untuk melakukan perilaku konsumtif.
Literature Review: Perbedaan Budaya Negara Individualis dan Kolektivis Antesenden Manajemen Privasi di Jejaring Sosial (SNS)
Authors: Sonia Bella Prastika  , Mutia Husna Avezahra
Published: August 2024

In this Literature Review, we examine the psychological antecedents of privacy management strategies on social networking (SNS) sites and expand our understanding of collective privacy management by using articles that review various individualistic and collectivistic countries. In these countries, there are differences regarding privacy management in social networks (SNS). Articles were collected using the systematic review method through a search process on Google Scholar to sort articles according to the topic of privacy management in individualistic and collectivistic countries. The results of the articles are based on predetermined categories, namely based on point results (Dutch America), (German America), (Korean Chinese America), and (Singaporean American Korean). So, in this case, it is proven that America, as an individualistic country, holds the highest privacy management power compared to collectivistic countries such as China, Korea, the Netherlands, Germany, and Singapore. In addition, the authors found that, in general, users' privacy attitudes, social norms, and self- or collective control beliefs substantially predicted the adoption of privacy management strategies. These findings contribute to explaining the adoption of behavioral privacy management strategies by users. We conclude with global and country-specific recommendations regarding future privacy designs for privacy management.
AbstrakTujuan penulisan artikel ini adalah pemeriksaan anteseden psikologis dari strategi manajemen privasi di situs jejaring sosial (SNS) dan memperluas pemahaman untuk manajemen privasi kolektif. Menggunakan artikel yang mengulas Negara individualistik dan kolektivistik. Terlihat adanya perbedaan pada Negara tersebut mengenai manajemen privasi di jejaring sosial (SNS). Artikel dikumpulkan menggunakan metode systematic review melalui proses pencarian pada google scholar dan memilah artikel yang sesuai dengan topik manajemen privasi di Negara individualistik dan kolektivistik. Hasil artikel berdasarkan kategori yang telah ditentukan yaitu berdasarkan point result (Amerika Belanda), (Amerika Jerman), (Amerika Cina Korea) (Amerika Singapura Korea). Sehingga dalam hal ini terbukti bahwa Amerika sebagai Negara individualistik memegang kekuasaan manajemen privasi tertinggi dibandingkan dengan Negara koletivistik seperti cina, korea, belanda, jerman dan singapura. Selain itu, penulis menemukan bahwa secara umum sikap privasi pengguna, norma sosial, dan keyakinan kontrol diri atau kolektif secara substansial memprediksi penerapan strategi manajemen privasi. Temuan ini berkontribusi pada penjelasan penerapan strategi manajemen privasi perilaku oleh pengguna.
Are We Different?, Tinjauan Kepribadian secara Lintas Budaya dalam Implikasinya terhadap Kehidupan Manusia
Authors: Rayza Ilfie Azkya Ashgarie  , Mutia Husna Avezahra
Published: August 2024

This article aims to find out personality with cross-cultural views and its various implications for human life. The process of compiling this article uses a qualitative approach in the form of a literature review. The results obtained are that cross-cultural personality has at least implications for changes in personality, maturity, religiosity, culture shock, motivation and cognition, enjoyment of entertainment, cultural intelligence, bullying behavior, subjective well-being, and self-representation. In addition, intercultural personality differences were also obtained on the continents of Asia vs. Europe, Asia vs. America, and Europe vs. America. Based on the implications, there are three levels of implications, namely cultural differences in personality affect these implications differently, such as changes in personality; cultural differences in personality affect these implications in the same way, such as enjoying entertainment; and cultural differences in personality do not greatly affect these implications such as bullying behavior. Meanwhile, by region, Asia tends to be collectivist, so it has a closer personality than Europe and America. America tends to be individual, so it is more open than Asia and Europe, and Europe can be both collectivist and individualist depending on who the comparison is.
AbstrakArtikel ini bertujuan untuk mengetahui kepribadian dengan tinjauan lintas budaya dalam berbagai implikasinya terhadap kehidupan manusia. Proses penyusunan artikel ini menggunakan pendekatan kualitatif berjenis literature review. Adapun hasil yang diperoleh yakni kepribadian secara lintas budaya berimplikasi setidaknya terhadap perubahan kepribadian, kedewasaan, religiusitas, culture shock, motivasi dan kognitif, menikmati hiburan, kecerdasan budaya, perilaku bullying, subjective well-being, dan representasi diri. Selain itu, diperoleh juga perbedaan kepribadian antarbudaya secara benua Asia vs Eropa, Asia vs Amerika, dan Eropa vs Amerika. Berdasarkan implikasi, terdapat tiga tingkat implikasi yakni perbedaan kepribadian secara budaya mempengaruhi implikasi tersebut secara berbeda pula seperti perubahan kepribadian, perbedaan kepribadian secara budaya mempengaruhi implikasi tersebut secara sama seperti menikmati hiburan, dan perbedaan kepribadian secara budaya tidak terlalu mempengaruhi implikasi tersebut seperti perilaku bullying. Sedangkan berdasarkan wilayah, Asia cenderung kolektivis sehingga berkepribadian tertutup daripada Eropa dan Amerika, Amerika cenderung individual sehingga lebih terbuka daripada Asia dan Eropa, dan Eropa dapat bersifat kolektivis dan individualis bergantung pada siapa pembandingnya.