bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Hooked-End Steel Fiber Geometry and Volume Fraction on the Flexural Behavior of Concrete Pedestrian Decks
Authors: Lee Seung-Jung , Yoo Doo-Yeol , Moon Do-Young
Published: March 2019

This study investigates the effects of hooked-end fiber geometry and volume fraction on the flexural behavior of concrete pedestrian decks. To achieve this, three different fiber geometries, i.e., three-dimensional (3D), four-dimensional (4D), and five-dimensional (5D), and volume fractions of 0.37%, 0.6%, and 1.0% were considered. Test results indicate that a higher number of hook ends can more effectively enhance the flexural strength and flexural strength margin at all volume fractions than a lower number, so that the order of effectiveness of hooked-end fibers on the flexural strength parameters was as follows: 5D > 4D > 3D. To satisfy the ductility index of 0.39, the amounts of 3D, 4D, and 5D hooked steel fibers should be in the range of 0.98%‒1.10%. Moreover, at a fiber volume fraction of 1.0%, only multiple cracking behaviors were observed, and the numerical results indicated that the volume fraction should be equal to 1.0% to guarantee a deflection-hardening response of pedestrian decks, regardless of the hooked-end fiber geometry. Consequently, a 1.0% by volume of hooked-end steel fiber is recommended to replace the minimum longitudinal steel rebars and guarantee a ductile flexural behavior with multiple cracks for pedestrian decks made of high-strength concrete.
Record. Reenact. Recycle. Notes on Shindō Kaneto’s Documentary Styles
Authors: Kitsnik Lauri
Published: March 2019

In his work, the filmmaker Shindō Kaneto sought to employ various, often seemingly incongruous, cinematic styles that complicate the notions of fiction and documentary film. This paper first examines his ‘semi-documentary’ films that often deal with the everyday life of common people by means of an enhanced realist approach. Second, attention is paid to the fusion of documentary and drama when reenacting historical events, as well as the subsequent recycling of these images in a ‘quasi-documentary’ fashion. Finally, I uncover a trend towards ‘meta-documentary’ that takes issue with the act of filmmaking itself. I argue that Shindō’s often self-referential work challenges the boundaries between fiction and non-fiction while engaging in a self-reflective criticism of cinema as a medium.
Simulating Sundowner Winds in Coastal Santa Barbara: Model Validation and Sensitivity
Authors: Duine Gert-Jan , Jones Charles , Carvalho Leila M.V. , Fovell Robert G.
Published: March 2019

This study investigates the influence of planetary boundary layer (PBL) schemes and land surface models (LSMs) on the performance of the Weather Research & Forecasting model in simulating the development of downslope windstorms in the lee of the Santa Ynez Mountains in Santa Barbara, California (known as Sundowner winds). Using surface stations, a vertical wind profiler, and a multi-physics ensemble approach, we found that most of the wind speed biases are controlled by the roughness length z 0 , and so by the choice of LSM. While timing characteristics of Sundowners are insensitive to both LSM and PBL schemes, a clear sensitivity in the horizontal extent of strong surface winds is found for both PBL parameterization and z 0 , which are related to patterns of self-induced wave-breaking near the mountaintop, and the erosion of the marine layer. These results suggest that LSMs with relatively high values of z 0 , and TKE-based or hybrid PBL schemes adequately simulate downslope windstorms in the lee of mountain ranges, specifically in areas where downslope windstorms interact with the marine boundary layer with stably stratified characteristics.
Size-Segregated Characteristics of Carbonaceous Aerosols during the Monsoon and Non-Monsoon Seasons in Lhasa in the Tibetan Plateau
Authors: Wei Nannan , Ma Chulei , Liu Junwen , Wang Guanghua , Liu Wei , Zhuoga Deqing , Xiao Detao , Yao Jian
Published: March 2019

In this paper, we intensively collected atmospheric particulate matter (PM) with different diameters (size ranges: <0.49, 0.49–0.95, 0.95–1.5, 1.5–3.0, 3.0–7.2, and >7.2 μm) in Lhasa during the monsoon and non-monsoon seasons. The results clearly showed that the concentrations of PM, organic carbon (OC), elemental carbon (EC), and water-soluble organic carbon (WSOC) during the non-monsoon season were much higher than the concentrations during the monsoon season. During the monsoon season, a bimodal size distribution of the OC and WSOC, which were at <0.49 μm and >7.2 μm, respectively, and a unimodal size distribution at <0.49 μm for the EC were observed. However, during the non-monsoon season, there was a trimodal size distribution of the OC and WSOC (<0.49 μm, 1.5–3.0 μm, and >7.2 μm), and a unimodal size distribution of the EC (<0.49 μm). Possible sources of the carbonaceous components were revealed by combining the particle size distribution and the correlation analysis. OC, EC, and WSOC were likely from the photochemical transformation of biogenic and anthropogenic VOC, and the incomplete combustion of biomass burning and fossil fuels at <0.49 μm, whilst they were also likely to be from various types of dust and biogenic aerosols at >7.2 μm. OC and WSOC at 1.5–3.0 μm were likely to have been from the burning of yak dung and photochemical formation. The above results may draw attention in the public and scientific communities to the issues of air quality in the Tibetan Plateau.
Analysis of Severe Droughts in Taiwan and its Related Atmospheric and Oceanic Environments
Authors: Hung Chih-wen , Shih Ming-Fu
Published: March 2019

Drought is one of the important issues in climate studies. A drought index, Taiwan Meteorological Drought index (TMD index), was previously proposed and is applied here to identify historical severe droughts in Taiwan in order to clarify the corresponding large-scale backgrounds as a potential alert to the society in future. Through the TMD index, several historical severe drought cases in Taiwan are detected and characterized by significant seasonal variability in the annual cycle. Composites for large-scale atmospheric and oceanic environments over different periods within the dry season are conducted. From October to December, the colder sea surface temperature (SST) pattern of Pacific Meridional Mode (PMM) and the PMM-induced local anomalous anticyclones over the South China Sea are both in charge of the extremely dry conditions in Taiwan. From January to February, cold SST in the South China Sea and its adjacent oceans dominates local atmospheric conditions above these regions and creates an unfavorable environment for convection systems. From March to May, a massive anomalous anticyclonic circulation centering beside Alaska and extending its properties to East Asia and Taiwan generates a descending environment and in turn suppresses convection systems to develop. Therefore, the extremely dry conditions under this system are expected.
Oncostatin M Mediates Adipocyte Expression and Secretion of Stromal-Derived Factor 1
Authors: Hang Hardy , Bailey Jennifer L. , Elks Carrie M.
Published: March 2019

Adipose tissue homeostasis depends on interactions between stromal cells, adipocytes, and the cytokines and chemokines they produce. The gp130 cytokine, oncostatin M (OSM), plays a role in adipose tissue homeostasis. Mice, lacking the OSM receptor (OSMR) in adipocytes (OsmrFKO mice), exhibit derangements in adipose tissue, insulin sensitivity, and immune cell balance. Here, we describe a possible role for the chemokine stromal-derived factor 1 (SDF-1) in these alterations. We treated 3T3-L1 adipocytes with OSM and observed a suppression of SDF-1 gene expression and protein secretion, an effect which was partially blunted by OSMR knockdown. However, OsmrFKO mice also exhibited decreased SDF-1 gene and protein expression in adipose tissue. These contrasting results suggest that the loss of adipocyte OSM–OSMR signaling in vivo may be indirectly affecting adipokine production and secretion by altering OSM target genes to ultimately decrease SDF-1 expression in the OsmrFKO mouse. We conclude that adipocyte OSM–OSMR signaling plays a role in adipose tissue SDF-1 production and may mitigate its effects on adipose tissue homeostasis.
Metformin Induces Different Responses in Clear Cell Renal Cell Carcinoma Caki Cell Lines
Authors: Pasha Mazhar , Sivaraman Siveen K. , Frantz Ronald , Agouni Abdelali , Munusamy Shankar
Published: March 2019

Clear cell renal cell carcinoma (ccRCC) is the most common and lethal form of urological cancer diagnosed globally. Mutations of the von Hippel-Lindau (VHL) tumor-suppressor gene and the resultant overexpression of hypoxia-inducible factor (HIF)-1α protein are considered hallmarks of ccRCC. Persistently activated HIF-1α is associated with increased cell proliferation, angiogenesis, and epithelial–mesenchymal transition (EMT), consequently leading to ccRCC progression and metastasis to other organs. However, the VHL status alone cannot predict the differential sensitivity of ccRCC to cancer treatments, which suggests that other molecular differences may contribute to the differential response of ccRCC cells to drug therapies. In this study, we investigated the response to metformin (an antidiabetic drug) of two human ccRCC cell lines Caki-1 and Caki-2, which express wild-type VHL. Our findings demonstrate a differential response between the two ccRCC cell lines studied, with Caki-2 cells being more sensitive to metformin compared to Caki-1 cells, which could be linked to the differential expression of HIF-1α despite both cell lines carrying a wild-type VHL. Our study unveils the therapeutic potential of metformin to inhibit the progression of ccRCC in vitro. Additional preclinical and clinical studies are required to ascertain the therapeutic efficacy of metformin against ccRCC.
Spontaneous Switching among Conformational Ensembles in Intrinsically Disordered Proteins
Authors: Choi Ucheor , Sanabria Hugo , Smirnova Tatyana , Bowen Mark , Weninger Keith
Published: March 2019

The common conception of intrinsically disordered proteins (IDPs) is that they stochastically sample all possible configurations driven by thermal fluctuations. This is certainly true for many IDPs, which behave as swollen random coils that can be described using polymer models developed for homopolymers. However, the variability in interaction energy between different amino acid sequences provides the possibility that some configurations may be strongly preferred while others are forbidden. In compact globular IDPs, core hydration and packing density can vary between segments of the polypeptide chain leading to complex conformational dynamics. Here, we describe a growing number of proteins that appear intrinsically disordered by biochemical and bioinformatic characterization but switch between restricted regions of conformational space. In some cases, spontaneous switching between conformational ensembles was directly observed, but few methods can identify when an IDP is acting as a restricted chain. Such switching between disparate corners of conformational space could bias ligand binding and regulate the volume of IDPs acting as structural or entropic elements. Thus, mapping the accessible energy landscape and capturing dynamics across a wide range of timescales are essential to recognize when an IDP is acting as such a switch.
Assessing the Ability of Image Based Point Clouds Captured from a UAV to Measure the Terrain in the Presence of Canopy Cover
Authors: Wallace Luke , Bellman Chris , Hally Bryan , Hernandez Jaime , Jones Simon , Hillman Samuel
Published: March 2019

Point clouds captured from Unmanned Aerial Systems are increasingly relied upon to provide information describing the structure of forests. The quality of the information derived from these point clouds is dependent on a range of variables, including the type and structure of the forest, weather conditions and flying parameters. A key requirement to achieve accurate estimates of height based metrics describing forest structure is a source of ground information. This study explores the availability and reliability of ground surface points available within point clouds captured in six forests of different structure (canopy cover and height), using three image capture and processing strategies, consisting of nadir, oblique and composite nadir/oblique image networks. The ground information was extracted through manual segmentation of the point clouds as well as through the use of two commonly used ground filters, LAStools lasground and the Cloth Simulation Filter. The outcomes of these strategies were assessed against ground control captured with a Total Station. Results indicate that a small increase in the number of ground points captured (between 0 and 5% of a 10 m radius plot) can be achieved through the use of a composite image network. In the case of manually identified ground points, this reduced the root mean square error (RMSE) error of the terrain model by between 1 and 11 cm, with greater reductions seen in plots with high canopy cover. The ground filters trialled were not able to exploit the extra information in the point clouds and inconsistent results in terrain RMSE were obtained across the various plots and imaging network configurations. The use of a composite network also provided greater penetration into the canopy, which is likely to improve the representation of mid-canopy elements.
The Relationship between Stem Diameter Shrinkage and Tree Bole Moisture Loss Due to Transpiration
Authors: Tian Yuan , Zhang Qiuliang , Liu Xuan , Meng Meng , Wang Bing
Published: March 2019

The responsiveness of four types of stem diameter shrinkage indicators to sap flow changes was compared under four air temperature and cloudiness conditions: above 0 °C below 80% cloud cover days; above 0 °C large percentage cloud cover days; low temperature below 80% cloud cover days; and low temperature large percentage cloud cover days. In this study, we investigated the effects of indicative functions of relatively easy-to-access stem diameter shrinkage on variation characteristics of sap flow. High-resolution-based stem diameter shrinkage is related to changes in tree moisture content. Stem diameter shrinkage indicators are adopted to confirm sap flow changes resulting from transpiration pull, which may enhance the power of stem diameter shrinkage as an index for tree bole moisture loss. After measuring stem diameter variations, the following stem diameter shrinkage indicators were calculated: maximum daily shrinkage, daily stem diameter increment, daily stem diameter variation, and tree water deficit-induced stem shrinkage (TWD). Sap flow was measured synchronously, and stem diameter shrinkage indicators were analyzed to confirm their responses to sap flow. TWD was positively correlated (r ≥ 0.317) with daily variations in sap flow and reached extremely significant levels (p ≤ 0.001) under all conditions. TWD and maximum daily shrinkage were able to better reflect the correlation between changes in stem diameter and sap flow on a daily scale, except large percentage cloud cover days with low temperatures. Changes in stem diameter had no correlation with sap flow during low temperature and large percentage cloud cover days. Among all stem diameter shrinkage indicators, TWD showed the highest correlation (r ≥ 0.601 and p ≤ 0.001) with sap flow under all conditions, except during large percentage cloud cover days with low temperatures. The stem diameter shrinkage indicators did not reflect sap flow changes during large percentage cloud cover days with low temperatures. The indicator that best reflected moisture loss of trees was TWD.
A Numerical Investigation on Tidally Induced Sediment Transport and Morphological Changes with Changing Sea Level in South-East England
Authors: Leonardi Nicoletta , Li Xiaorong , Carnacina Iacopo
Published: March 2019

The impact of tide-induced morphological changes and water level variations on the sediment transport in a tidally dominated system has been investigated using the numerical model Delft3D and South-East England as a test case. The goal of this manuscript is to explore the long-term changes in morphology due to sea level rise and the large-scale morphodynamic equilibrium of the South-East England. Our results suggest that the long term (century scale) tidally-induced morphological evolution of the seabed slows down in time and promotes a vanishing net transport across the large scale system. Century-scale morphologically updated simulations show that both morphological changes and net transport values tend to decrease in time as the system attains a dynamic equilibrium configuration. Results further suggest that the presence of a gradual increase in mean sea level accelerates the initial morphological evolution of the system whose morphological rate of change gradually attains, however, same plateau values as in the absence of sea level rise. Given the same base morphology, increasing water levels enhance residual currents and the net transport near the coastline; and vice-versa, decreasing sea levels minimize both residuals and net transport near the coastline. The areas that are more affected by, water level and morphological changes, are the ones where the net transport is the highest. This manuscript explores and allows extending the idea of morphodynamic equilibrium at a regional scale, larger than the one for which this concept has been generally explored i.e., estuarine scale.
Seafloor Classification in a Sand Wave Environment on the Dutch Continental Shelf Using Multibeam Echosounder Backscatter Data
Authors: Koop Leo , Amiri-Simkooei Alireza , J. van der Reijden Karin , O’Flynn Sarah , Snellen Mirjam , G. Simons Dick
Published: March 2019

High resolution maps of sandy seafloors are valuable to understand seafloor dynamics, plan engineering projects, and create detailed benthic habitat maps. This paper presents multibeam echosounder backscatter classification results of the Brown Bank area of the North Sea. We apply the Bayesian classification method in a megaripple and sand wave area with significant slopes. Prior to the classification, corrections are implemented to account for the slopes. This includes corrections on the backscatter value and its corresponding incident angle. A trade-off in classification resolutions is found. A higher geo-acoustic resolution is obtained at the price of losing spatial resolution, however, the Bayesian classification method remains robust with respect to these trade-off decisions. The classification results are compared to grab sample particle size analysis and classified video footage. In non-distinctive sedimentary environments, the acoustic classes are not attributed to only the mean grain size of the grab samples but to the full spectrum of the grain sizes. Finally, we show the Bayesian classification results can be used to characterize the sedimentary composition of megaripples. Coarser sediments were found in the troughs and on the crests, finer sediments on the stoss slopes and a mixture of sediments on the lee slopes.
“Divided by a Common Language”: The Use of Verbatim in Carol Ann Duffy and Rufus Norris’ My Country; A Work in Progress
Authors: O’Brien Shauna
Published: March 2019

‘My Country; A Work in Progress’ written and arranged by the poet Carol Ann Duffy is a verbatim play that uses interviews conducted with people from various regions in England, Wales, Scotland, and Northern Ireland to explore the causes of the EU referendum result. With the recent rise of populism across Europe, Britain, and America, an increased scepticism of established news media organisations, and a widespread disillusionment with the so-called political elite class, verbatim theatre’s “claim to veracity” and use of real-life testimonies seems to provide an attractive forum for both playwrights and audiences to investigate the underlying causes prompting these political and social movements. This paper examines how Duffy’s highly-fragmented arrangement of My Country’s verbatim voices in tandem with their re-citation and reterritorialization in the bodies of the performers on the stage ironically undermines the “claim to veracity” that its verbatim approach implies.
Spectral Sterility in Bucknill and Tuke’s A Manual of Psychological Medicine and Bulwer Lytton’s A Strange Story
Authors: Mera Ford Natalie
Published: March 2019

This essay identifies and examines a narrative structure—here called the sterility plot—that is shown to recur in British mid-19th century psychiatric texts and imaginative literature engaging mental science. Treating physicians Bucknill and Tuke’s A Manual of Psychological Medicine and novelist Bulwer Lytton’s A Strange Story as influential case studies, it explores in particular the Gothic-styled spectralisation used by both Victorian medical and literary authors to characterize females whose mental disorders are depicted as bound with a short- or long-term inability to reproduce. The narratives thereby gender, pathologise, and suspensefully dramatise the plot trajectory of mentally ill patients’ clinical and fictional case histories, which, taken together, is argued to reveal mid-century medico-cultural anxieties about the health of Britain’s imperial future being threatened by potentially sterile Englishwomen.
Two 1916s: Sebastian Barry’s A Long Long Way
Authors: Haas Allison
Published: March 2019

As Paul Fussell has shown, the First World War was a watershed moment for 20th century British history and culture. While the role of the 36th (Ulster) Division in the Battle of the Somme has become a part of unionist iconography in what is now Northern Ireland, the experience of southern or nationalist Irish soldiers in the war remains underrepresented. Sebastian Barry’s 2005 novel, A Long Long Way is one attempt to correct this historical imbalance. This article will examine how Barry represents the relationship between the First World War and the 1916 Easter Rising through the eyes of his politically-conflicted protagonist, Willie Dunne. While the novel at first seems to present a common war experience as a means of healing political divisions between Ireland and Britain, this solution ultimately proves untenable. By the end of the novel, Willie’s hybrid English–Irish identity makes him an outcast in both places, even as he increasingly begins to identify with the Irish nationalist cause. Unlike some of Barry’s other novels, A Long Long Way does not present a disillusioned version of the early 20th century Irish nationalism. Instead, Willie sympathizes with the rebels, and Barry ultimately argues for a more inclusive Irish national identity.
Organic Food Purchases in an Emerging Market: The Influence of Consumers’ Personal Factors and Green Marketing Practices of Food Stores
Authors: Nguyen Hoang Viet , Nguyen Ninh , Nguyen Bach Khoa , Lobo Antonio , Vu Phuong Anh
Published: March 2019

The consumption of food has a significant impact on the environment, individuals and public health. This study aims to investigate the integrative effects of consumers’ personal and situational factors on their attitude and purchase behavior of organic meat. The consumption of this product has been widely regarded as contributing towards sustainable food practices. The study was conducted in an emerging market economy, i.e., Vietnam. Data were collected using a customized and validated survey instrument from a sample of 609 organic meat consumers at four food outlets in Hanoi. The findings suggested that consumers’ concerns regarding the environment, health, food safety and their knowledge of organic food, all significantly impacted their attitude towards the purchase behavior of organic meat. Interestingly, their positive attitude did not necessarily translate into their actual purchase of organic meat. Additionally, food stores’ green marketing practices significantly enhanced consumers’ actual purchase behavior. Conversely, premium prices of organic meat were certainly a deterrent for the actual purchase of organic meat. The findings of this study have several important implications for organic food producers, retailers, policy makers and socio-environmental organizations that seek to develop intervention strategies aimed at increasing organic meat consumption in Vietnam.
Primary Cooking Fuel Choice and Respiratory Health Outcomes among Women in Charge of Household Cooking in Ouagadougou, Burkina Faso: Cross-Sectional Study
Authors: Sana Adama , Meda Nicolas , Badoum Gisèle , Kafando Benoit , Bouland Catherine
Published: March 2019

Background: Approximately 3 billion people, worldwide, rely primarily on biomass for cooking. This study aimed to investigate the association between respiratory symptoms among women in charge of household cooking and the type of fuel used for cooking. Methods: A community-based cross-sectional survey was conducted. A total of 1705 women that were randomly selected, completed the survey. We also performed a bivariate and a multivariate analysis to verify the possible associations between respiratory symptoms in women in charge of household cooking and the type of cooking fuel used. Results: Dry cough, breathing difficulties, and throat irritation frequencies were statistically high in biomass fuel users when compared to liquefied petroleum gas (LPG) users. It was also the case for some chronic respiratory symptoms, such as sputum production, shortness of breath, wheezing, wheezing with dyspnea, wheezing without a cold, waking up with shortness of breath, waking up with coughing attacks, and waking up with breathing difficulty. After adjustment for the respondents’ and households’ characteristics; dry cough, breathing difficulties, sneezing, nose tingling, throat irritation, chronic sputum production, wheezing, wheezing with dyspnea, wheezing without a cold, waking up with shortness of breath, waking up with coughing attacks, and waking up with breathing difficulty were symptoms that remained associated to biomass fuel compared to LPG. Women who used charcoal reported the highest proportion of all the chronic respiratory symptoms compared to the firewood users. However, this difference was not statistically significant except for the wheezing, waking up with coughing attacks, and waking up with breath difficulty, after adjustment. Conclusion: Exposure to biomass smoke is responsible for respiratory health problems in women. Charcoal, which is often considered as a clean fuel compared to other biomass fuels and often recommended as an alternative to firewood, also presents health risks, including increased respiratory morbidity in women. Effective and efficient energy policies are needed to accelerate the transition to clean and sustainable energies.
Public Perceptions of Environmental Public Health Risks in the United States
Authors: Shin Mikyong , Werner Angela K. , Strosnider Heather , Hines Lisa B. , Balluz Lina , Yip Fuyuen Y.
Published: March 2019

Understanding public perceptions about environmental health hazards, exposures, and health impacts can help environmental public health practitioners to target and prioritize community activities, policy needs, and communication strategies. The online cross-sectional 2013 summer wave of the ConsumerStyles survey sampled U.S. adults and used questions from the Centers for Disease Control’s Environmental Public Health Tracking Program to measure public awareness of governmental efforts to track environmental exposures and links to health impacts, as well as perceptions of environmental health issues. Unadjusted and adjusted logistic regressions examined the associations between demographic characteristics and level of awareness of government environmental public health efforts or level of concern about health risks associated with environmental pollutants. Responses were received from 4033 participants, yielding a response rate of 66.0%. More than half of respondents (57.8%) noted concerns about health risks from environmental pollutants. More than one-third (40.0%) of respondents reported awareness of government efforts. Nearly 40% of respondents felt that none of the health impacts listed in the survey were related to environmental issues. Multiple logistic regression models showed that non-Hispanic blacks, other races, females, people with a college or higher education, and people living in the Midwest or South regions were more likely than their counterparts to be concerned about how the environment affects their health. Future work should focus on improving risk communication, filling the information gap on environmental health issues, and understanding how perceptions change over time.
Immigrant–Native Differences in Sugar-Sweetened Beverage and Snack Consumption and Preventive Behaviors Associated with Severe Early Childhood Caries: A Large-Scale Survey in Taiwan
Authors: Lin Ying-Chun , Chang Chin-Shun , Ho Pei-Shan , Lee Chien-Hung , Chen Jen-Hao , Huang Hsiao-Ling
Published: March 2019

This study assessed the differences between immigrants and natives in terms of combined effects of sugar-sweetened beverage (SSB) or snack consumption and preventive behaviors for severe early childhood caries (SECC) through a large-scale survey of 31,565 native and 1046 immigrant child–parent pairs in Taiwan. Children aged 3–5 years underwent dental examinations, and parents completed structured questionnaires. Immigrants had a significantly higher SECC prevalence than native children (32.3% vs. 19.4%). A combined effect of SECC was observed in native children who did not receive assistance when brushing teeth at night before sleep and those who consumed SSBs more than four times weekly (adjusted odds ratio (aOR) = 4.8). Moreover, native children who did not use dental floss and who consumed snacks more than four times weekly had an aOR of 4.1 for SECC. The combined effects of children with immigrant parents who did not receive assistance when brushing their teeth at night before sleep and those who consumed snacks more than four times weekly had an aOR of 8.2 for SECC. The results suggest the necessity of cross-cultural caries prevention programs for immigrants. Parents must limit children’s SSB and snack intake, and implement preventive measures to reduce SECC development.
Airbnb Offer in Spain—Spatial Analysis of the Pattern and Determinants of Its Distribution
Authors: Adamiak Czesław , Szyda Barbara , Dubownik Anna , García-Álvarez David
Published: March 2019

The rising number of homes and apartments rented out through Airbnb and similar peer-to-peer accommodation platforms cause concerns about the impact of such activity on the tourism sector and property market. To date, spatial analysis on peer-to-peer rental activity has been usually limited in scope to individual large cities. In this study, we take into account the whole territory of Spain, with special attention given to cities and regions with high tourist activity. We use a dataset of about 250 thousand Airbnb listings in Spain obtained from the Airbnb webpage, aggregate the numbers of these offers in 8124 municipalities and 79 tourist areas/sites, measure their concentration, spatial autocorrelation, and develop regression models to find the determinants of Airbnb rentals’ distribution. We conclude that apart from largest cities, Airbnb is active in holiday destinations of Spain, where it often serves as an intermediary for the rental of second or investment homes and apartments. The location of Airbnb listings is mostly determined by the supply of empty or secondary dwellings, distribution of traditional tourism accommodation, coastal location, and the level of internationalization of tourism demand.
Location Privacy in the Wake of the GDPR
Authors: Georgiadou Yola , de By Rolf A. , Kounadi Ourania
Published: March 2019

The General Data Protection Regulation (GDPR) protects the personal data of natural persons and at the same time allows the free movement of such data within the European Union (EU). Hailed as majestic by admirers and dismissed as protectionist by critics, the Regulation is expected to have a profound impact around the world, including in the African Union (AU). For European–African consortia conducting research that may affect the privacy of African citizens, the question is ‘how to protect personal data of data subjects while at the same time ensuring a just distribution of the benefits of a global digital ecosystem?’ We use location privacy as a point of departure, because information about an individual’s location is different from other kinds of personally identifiable information. We analyse privacy at two levels, individual and cultural. Our perspective is interdisciplinary: we draw from computer science to describe three scenarios of transformation of volunteered or observed information to inferred information about a natural person and from cultural theory to distinguish four privacy cultures emerging within the EU in the wake of GDPR. We highlight recent data protection legislation in the AU and discuss factors that may accelerate or inhibit the alignment of data protection legislation in the AU with the GDPR.
Pseudophosphatase MK-STYX Alters Histone Deacetylase 6 Cytoplasmic Localization, Decreases Its Phosphorylation, and Increases Detyrosination of Tubulin
Authors: Cao Yuming , Banks Dallas A. , Mattei Andrew M. , Riddick Alexys T. , Reed Kirstin M. , Zhang Ashley M. , Pickering Emily S. , Hinton Shantá D.
Published: March 2019

The catalytically inactive mitogen-activated protein (MAP) kinase phosphatase, MK-STYX (MAPK (mitogen-activated protein kinase) phosphoserine/threonine/tyrosine-binding protein) interacts with the stress granule nucleator G3BP-1 (Ras-GAP (GTPase-activating protein) SH3 (Src homology 3) domain-binding protein-1), and decreases stress granule (stalled mRNA) formation. Histone deacetylase isoform 6 (HDAC6) also binds G3BP-1 and serves as a major component of stress granules. The discovery that MK-STYX and HDAC6 both interact with G3BP-1 led us to investigate the effects of MK-STYX on HDAC6 dynamics. In control HEK/293 cells, HDAC6 was cytosolic, as expected, and formed aggregates under conditions of stress. In contrast, in cells overexpressing MK-STYX, HDAC6 was both nuclear and cytosolic and the number of stress-induced aggregates significantly decreased. Immunoblots showed that MK-STYX decreases HDAC6 serine phosphorylation, protein tyrosine phosphorylation, and lysine acetylation. HDAC6 is known to regulate microtubule dynamics to form aggregates. MK-STYX did not affect the organization of microtubules, but did affect their post-translational modification. Tubulin acetylation was increased in the presence of MK-STYX. In addition, the detyrosination of tubulin was significantly increased in the presence of MK-STYX. These findings show that MK-STYX decreases the number of HDAC6-containing aggregates and alters their localization, sustains microtubule acetylation, and increases detyrosination of microtubules, implicating MK-STYX as a signaling molecule in HDAC6 activity.
Therapeutic Targeting of Collective Invasion in Ovarian Cancer
Authors: Moffitt Laura , Karimnia Nazanin , Stephens Andrew , Bilandzic Maree
Published: March 2019

Ovarian cancer is the seventh most commonly diagnosed cancer amongst women and has the highest mortality rate of all gynaecological malignancies. It is a heterogeneous disease attributed to one of three cell types found within the reproductive milieu: epithelial, stromal, and germ cell. Each histotype differs in etiology, pathogenesis, molecular biology, risk factors, and prognosis. Furthermore, the origin of ovarian cancer remains unclear, with ovarian involvement secondary to the contribution of other gynaecological tissues. Despite these complexities, the disease is often treated as a single entity, resulting in minimal improvement to survival rates since the introduction of platinum-based chemotherapy over 30 years ago. Despite concerted research efforts, ovarian cancer remains one of the most difficult cancers to detect and treat, which is in part due to the unique mode of its dissemination. Ovarian cancers tend to invade locally to neighbouring tissues by direct extension from the primary tumour, and passively to pelvic and distal organs within the peritoneal fluid or ascites as multicellular spheroids. Once at their target tissue, ovarian cancers, like most epithelial cancers including colorectal, melanoma, and breast, tend to invade as a cohesive unit in a process termed collective invasion, driven by specialized cells termed “leader cells”. Emerging evidence implicates leader cells as essential drivers of collective invasion and metastasis, identifying collective invasion and leader cells as a viable target for the management of metastatic disease. However, the development of targeted therapies specifically against this process and this subset of cells is lacking. Here, we review our understanding of metastasis, collective invasion, and the role of leader cells in ovarian cancer. We will discuss emerging research into the development of novel therapies targeting collective invasion and the leader cell population.
The Toxic Effects and Mechanisms of Nano-Cu on the Spleen of Rats
Authors: Zhou Xuerong , Zhao Ling , Luo Jie , Tang Huaqiao , Xu Min , Wang Yanyan , Yang Xiaoyu , Chen Helin , Li Yinglun , Ye Gang , Shi Fei , Lv Cheng , Jing Bo
Published: March 2019

Research has shown that nano-copper (nano-Cu) can cause damage to the spleen and immune system yet their mechanisms of cytotoxicity are poorly understood. Our aim is to explore the potential immunotoxicity in the spleen of rats after nano-Cu exposure. The results of hematologic parameters, lymphocyte subsets, immunoglobulins, and histopathology indicated that copper obviously changed the immune function of the spleen. The levels of antioxidants (SOD, CAT, GSH-Px), oxidants (iNOS, NO, MDA), and anti-oxidative signalling pathway of Nrf2 (Nrf2 and HO-1) were strongly induced by nano-Cu. The expression of mRNA and protein of pro-/anti-inflammatory (IFN-γ, TNF-α, MIP-1α, MCP-1, MIF, IL-1/-2/-4/-6) cytokines were increased by nano-Cu. The expression of regulatory signal pathways, MAPKs and PI3-K/Akt were activated, which might be involved in the inflammatory responses and immunomodulatory processes of sub-acute nano-Cu exposure. The immune function of the spleen was repressed by nano-Cu induced oxidative stress and inflammation.
Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice
Authors: Liu Peng , Yao Ruizhi , Shi Hongzhao , Liu Yang , Lian Shuai , Yang Yuying , Yang Huanmin , Li Shize
Published: March 2019

Cold-inducible RNA-binding protein (CIRP) is a stress-responsive protein involved in several signal transduction pathways required for cellular function, which are associated with apoptosis and proliferation. The present study aimed to investigate the possible effects of CIRP-mediated regulation of glucose metabolism in the liver following acute cold exposure. The livers and serum of male C57BL/6 mice were collected following cold exposure at 4 °C for 0 h, 2 h, 4 h, and 6 h. Glucose metabolic markers and the expression of glucose metabolic-related proteins were detected in the liver. Acute cold exposure was found to increase the consumption of glycogen in the liver. Fructose-1,6-diphosphate (FDP) and pyruvic acid (PA) were found to show a brief increase followed by a sharp decrease during cold exposure. Anti-apoptotic protein (Bcl-2) expression was upregulated. CIRP protein expression displayed a sequential increase with prolonged acute cold exposure time. Acute cold exposure also increased the level of protein kinase B (AKT) phosphorylation, and activated the AKT-signaling pathway. Taken together, these findings indicate that acute cold exposure increased the expression of CIRP protein, which regulates mouse hepatic glucose metabolism and maintains hepatocyte energy balance through the AKT signaling pathway, thereby slowing the liver cell apoptosis caused by cold exposure.
5-HT3 Antagonist Ondansetron Increases apoE Secretion by Modulating the LXR-ABCA1 Pathway
Authors: Shinohara Motoko , Shinohara Mitsuru , Zhao Jing , Fu Yuan , Liu Chia-Chen , Kanekiyo Takahisa , Bu Guojun
Published: March 2019

Apolipoprotein E (apoE) is linked to the risk for Alzheimer’s disease (AD) and thus has been suggested to be an important therapeutic target. In our drug screening effort, we identified Ondansetron (OS), an FDA-approved 5-HT3 antagonist, as an apoE-modulating drug. OS at low micromolar concentrations significantly increased apoE secretion from immortalized astrocytes and primary astrocytes derived from apoE3 and apoE4-targeted replacement mice without generating cellular toxicity. Other 5-HT3 antagonists also had similar effects as OS, though their effects were milder and required higher concentrations. Antagonists for other 5-HT receptors did not increase apoE secretion. OS also increased mRNA and protein levels of the ATB-binding cassette protein A1 (ABCA1), which is involved in lipidation and secretion of apoE. Accordingly, OS increased high molecular weight apoE. Moreover, the liver X receptor (LXR) and ABCA1 antagonists blocked the OS-induced increase of apoE secretion, indicating that the LXR-ABCA1 pathway is involved in the OS-mediated facilitation of apoE secretion from astrocytes. The effects of OS on apoE and ABCA1 were also observed in human astrocytes derived from induced pluripotent stem cells (iPSC) carrying the APOE ε3/ε3 and APOE ε4/ε4 genotypes. Oral administration of OS at clinically-relevant doses affected apoE levels in the liver, though the effects in the brain were not observed. Collectively, though further studies are needed to probe its effects in vivo, OS could be a potential therapeutic drug for AD by modulating poE metabolism through the LXR-ABCA1 pathway.
Efficacy and Metabolic Effect on Serum Lipids of Apremilast in Psoriatic Arthritis: A Case Report
Authors: Gualtierotti Roberta , De Lucia Orazio
Published: March 2019

Psoriatic arthritis (PsA) is a chronic immune-mediated disease manifesting as joint inflammation with functional impairment associated with psoriasis. Recently, PsA has emerged as a systemic disease with several comorbidities, such as cardiovascular diseases and metabolic disorders. Apremilast is a targeted synthetic disease-modifying anti-rheumatic drug (tsDMARD) directed against phosphodiesterase 4 (PDE4) with demonstrated efficacy and safety in PsA and psoriasis. We report the case of a patient with PsA manifesting as arthritis, dactylitis, mild psoriasis and a significantly reduced health-related quality of life (HRQoL). Treatment with apremilast in association with methotrexate led to a quick improvement of joint and skin involvement with a stable amelioration of HRQoL. Furthermore, we observed a persistent favorable shift of serum lipid profile. Our observations suggest that apremilast is effective in controlling mild skin and joint involvement, including dactylitis, and suggest a potentially advantageous metabolic effect in patients with PsA.
Efficacy and Safety of Intravenous Thrombolysis in Patients with Acute Ischemic Stroke and Pre–Existing Disability
Authors: Merlino Giovanni , Corazza Elisa , Lorenzut Simone , Gigli Gian Luigi , Cargnelutti Daniela , Valente Mariarosaria
Published: March 2019

Little is known about intravenous thrombolysis (IVT) in acute ischemic stroke (AIS) patients with pre-existing disability. Disabled patients are often excluded from IVT treatment. Previous studies investigated the role of pre-existing disability on outcomes in AIS patients after IVT. However, no studies have been conducted to date to determine whether IVT may improve clinical outcomes in AIS patients with pre-existing disability. The aim of our study was to evaluate efficacy and safety of IVT in patients with pre-existing moderate and moderately severe disability (pre-stroke modified Rankin Scale score = 3 or 4) affected by AIS. This study was based on a retrospective analysis of a prospectively collected database of consecutive patients admitted to the Udine University Hospital with AIS from January 2015 to May 2018. The efficacy endpoints were the rate of favorable outcome and rate of major neurological improvement. The safety endpoints were the rate of mortality at three months, presence of intracranial hemorrhage (ICH), and presence of symptomatic intracranial hemorrhage (sICH). The study population included 110 AIS patients with pre-existing moderate and moderately severe disability, 36 of which received (IVT+) and 74 did not receive IVT (IVT−). AIS disabled patients treated with IVT had higher rates of favorable outcome (66.7% vs. 36.5%, p = 0.003) and major neurological improvement (39.4% vs. 17.4%, p = 0.01) compared to non-treated ones. Two in three disabled patients returned to their pre-stroke functional status when treated with IVT. Prevalence of three-month mortality, ICH, and sICH did not differ in the two groups. Disabled patients affected by AIS significantly improved after IVT. Moderate and moderately severe disability alone should not be considered, per se, as a contraindication to IVT treatment.
Daylight Saving Time and Acute Myocardial Infarction: A Meta-Analysis
Authors: Manfredini Roberto , Fabbian Fabio , Cappadona Rosaria , De Giorgi Alfredo , Bravi Francesca , Carradori Tiziano , Flacco Maria Elena , Manzoli Lamberto
Published: March 2019

Background: The available evidence on the effects of daylight saving time (DST) transitions on major cardiovascular diseases is limited and conflicting. We carried out the first meta-analysis aimed at evaluating the risk of acute myocardial infarction (AMI) following DST transitions. Methods: We searched cohort or case-control studies evaluating the incidence of AMI, among adults (≥18 y), during the weeks following spring and/or autumn DST shifts, versus control periods. The search was made in MedLine and Scopus, up to 31 December 2018, with no language restriction. A summary odds ratio of AMI was computed after: (1) spring, (2) autumn or (3) both transitions considered together. Meta-analyses were also stratified by gender and age. Data were combined using a generic inverse-variance approach. Results: Seven studies (>115,000 subjects) were included in the analyses. A significantly higher risk of AMI (Odds Ratio: 1.03; 95% CI: 1.01–1.06) was observed during the two weeks following spring or autumn DST transitions. However, although AMI risk increased significantly after the spring shift (OR: 1.05; 1.02–1.07), the incidence of AMI during the week after winter DST transition was comparable with control periods (OR 1.01; 0.98–1.04). No substantial differences were observed when the analyses were stratified by age or gender. Conclusion: The risk of AMI increases modestly but significantly after DST transitions, supporting the proposal of DST shifts discontinuation. Additional studies that fully adjust for potential confounders are required to confirm the present findings.