bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential of isothiocyanate sulforaphane from broccoli to combat obesity and type 2 diabetes: involvement of NRF2 regulatory pathway
Authors:   Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine, Ivanochko M. V. , Bayliak M. M. ,   Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;, Lushchak V. I. ,   Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;,   Research and Development University, Ivano-Frankivsk, Ukraine
Published: December 2024

Biologically active food components are now considered to be remedies for the prevention and treatment of metabolic disorders of different etiology. The aim of this review was to analyze the current data on the application of isothiocyanate sulforaphane, found in broccoli and other cruciferous plants, for the treatment of T2DM, obesity, and their comorbidities with the presentation of established molecular, particularly dependent on NF-E2-related factor-2 (Nrf2), and signaling mechanisms of therapeutic effects.

Keywords: broccoli sprouts, Nrf2, nutritional therapy, obesity, preventive health, sulforaphane, type 2 diabetes mellitus
Generation of the MCF-7 cell sublines with CRISPR/Cas9 mediated disruption of estrogen receptor alfa (ESR1) expression
Authors:   Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Savinska L. O. , Kvitchenko S. A. ,   Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;,   ESC “Institute of Biology and Medicine”, Taras Shevchenko National Univercity of Kyiv, Ukraine;, Palchevskyi S. S. ,   Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;, Kroupskaya I. V. ,   Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;, Mazov A. V. ,   Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;, Garifulin O. M. ,   Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;, Filonenko V. V. ,   Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
Published: December 2024

Supported by the literature, our initial hypothesis was that Estrogen Receptor alfa (ESR1) may function as a master regulator by influencing the expression of epithelial-to-mesenchymal transition (EMT)-related genes in cancer cells. To explore this further, we used the CRISPR/Cas9 gene editing system to create MCF-7 sublines with down-regulated ESR1 expression and analyzed its impact on EMT initiation. By applying two distinct types of gRNA for gene editing, we established six MCF-7 cell sublines with either nearly complete or partial down-regulation of the ESR1 isoforms. Unexpectedly, the data obtained revealed no discernible impact of ESR1 down-regulation on EMT manifestation as Western blot and Real-Time qPCR analysis of selected clones revealed no changes in EMT markers expression. We suggested that those of the ESR1 isoforms, the expression of which was not affected by gene editing, could be crucial for the initiation of EMT. The obtained cell models will be used further to evaluate the activity of ESR1 isoforms.

Keywords: CRISPR/Cas9, epithelial-to-mesenchymal transition, estrogen receptor alfa, MCF-7 cells
A halálos tűzesetek számának csökkentése és a kritikus kockázati csoportok védelme egyszerűsített tűzkockázat-elemzéssel
Authors: Hábermayer Tamás , Varga István , Kricskovics Antal , Vénosz Miklós
Published: December 2024

Magyarországon évente közel száz főre tehető azoknak az állampolgároknak a száma, akik tűzesetek következtében vesztik életüket. A statisztika szerint az érintettek többségében 50 év feletti férfiak, mentális, alkohol- vagy drogproblémákkal küzdők, gondozásra és felügyeletre szoruló idős személyek, illetve fogyatékkal élők vagy hajléktalanok. Ők alkotják a tűzhalál kritikus kockázati csoportját. Környezetükben mindenkitől fokozott figyelmet igényel a tüzek megelőzése, hiszen életkorukból, mentális állapotukból, anyagi helyzetükből következően ebben a körben jóval nagyobb a tűzesetek és ezek nyomán a halálesetek bekövetkezésének esélye. A szerzők engedély birtokában elemezték az elmúlt öt év tűzvizsgálatainak statisztikáit, és szakmai tapasztalataikra alapozva modern kockázatcsökkentési lehetőségeket javasolnak. A cikk megírásával céljuk a tűzhalálesetek számának csökkentése.
In-depth exploration of the correlation between metabolomic profiles and sensory attributes in Guangxi tea cultivars via LC-QTOF/MS analysis
Authors: Wang Jing , Li Yong , Liu Huahong , Liu Zhusheng , Huang Yingyi , Pang Yuelan , Wei Liuhua , Zhang Yun , Nie Jinfang
Published: December 2024

Tea's beneficial effects have long been attributed to its abundance of natural bioactive compounds; however, the influence of variations in these compounds on taste and aroma remains poorly understood. To investigate the relationship between metabolomic profiles and tea processing adaptability, this study employed sensory evaluation and untargeted metabolomics to compare four tea cultivars from Guangxi—Ying Hong No.9 (YH), Gui Hong No.3 (GH), Gui Cha No.2 (GC), and Mei Zhan (MZ)—cultivated under the same conditions. Sensory evaluation revealed that GH and MZ exhibited the highest quality in green tea and black tea, respectively, while GC showed moderate quality, and YH performed less favorably than the other three cultivars. Metabolomics analysis uncovered significant differences in the metabolic profiles among the four tea cultivars, with MZ and YH displaying higher levels of flavonol glycosides, and GC and GH showing elevated levels of organic acids and fatty acids. Pathway analyses of GH and MZ indicated that the differentially enriched pathways were primarily related to linoleic acid metabolism and flavonoid and flavanol synthesis. Correlation analyses further revealed that the sensory quality of green tea was closely linked to fatty acids and organic acids in fresh leaves, whereas the sensory quality of black tea was strongly associated with flavonol glycoside levels. This study provides a comprehensive understanding of the complex relationship between metabolite characteristics and processing suitability in different tea cultivars, offering valuable insights into the development of tea germplasm resources in the Guangxi region.
Filozoficzne aspekty teorii chaosu
Authors: Poznański Jacek
Published: December 2024

-
Sin normas ni estilo. Poemario juvenil
Authors:   Universidad Politécnica Salesiana, Ecuador,   Universidad Central del Ecuador
Published: December 2024

La poesía captura emociones y sentimientos que se materializan, a través de las palabras, en ideas o pensamientos profundos. Lo que para la pintura o el dibujo son los colores y las formas, para la poesía son los versos y las palabras. La poesía es el espejo porque en él se reflejan sentimientos, pensamientos o experiencias que se exponen a un lector quien, a su vez, se mira en él y, en muchos casos, se identifica. Estas características las podemos encontrar en el poemario Sin normas ni estilo. Un rico conjunto de imágenes y mensajes creados por 19 jóvenes para un número infinito de seres, con el único fin de compartir, desde la intimidad del lenguaje, sus mundos sutiles y, a la vez, intensos y poderosos.
УСЛОВИЯ ФОРМИРОВАНИЯ БЛАГОПОЛУЧИЯ РОССИЙСКИХ СЕМЕЙ (ПО РЕЗУЛЬТАТАМ АНАЛИЗА ОБРАЩЕНИЙ НА ФЕДЕРАЛЬНЫЙ НОМЕР СЕМЕЙНОЙ ЛИНИИ)
Authors: Васильева Екатерина Николаевна , Филина Мария Леонидовна
Published: December 2024

Целью исследования является систематизация данных о проблемах, препятствующих формированию благополучия российских семей, основные задачи — уточнение проблем разных типов семей, препятствующих благополучию; систематизация эмпирических данных, полученных в результате обращения россиян на федеральный номер Семейной линии; выявление специфики обращений, поступивших от членов разных типов семей.
Методологическую базу исследования составляют концепция благополучия семьи и теория социального капитала. Эмпирические данные систематизированы методами контент-анализа и качественного анализа документов.
Результаты исследования. Авторами проведен анализ проблем, с которым акторы сталкиваются в повседневной жизни, что позволило расширить представления о структуре факторов, влияющих на благополучие российских семей. В результате выявлены типичные и неспецифические ситуации, в которых оказываются семьи; систематизированы данные и ранжированы проблемы, препятствующие благополучию российских семей. На первом месте — инвалидность членов семьи, далее — ущемление прав детей, доступность и качество медицинской помощи, назначение и размер пенсионных начислений и т. д. Выявленные проблемы определяются как внешними условиями (институциональными) функционирования семьи, так и внутренними проблемами, обусловленными внутренними процессами в семье. Наибольшее влияние на внутренние проблемы оказывают конфликтные ситуации, малообеспеченность, неинформированность акторов и т. д. Преодолеть внутренние проблемы позволяет использование социального капитала некоммерческих организаций, имеющих информационные и институциональные ресурсы для поддержки семей не только в типичных ситуациях, но и в ситуациях, требующих использования метода кейс-стади и привлечения ресурсов межведомственного взаимодействия, участия государственных учреждений (системы социальной защиты, медицинской помощи и др.).
Перспективы исследования заключаются в использовании полученных данных для углубления знаний о повседневных практиках российских семей, нуждающихся в социальной поддержке, в том числе многодетных российских семей, а также для разработки управленческих решений, направленных на формирование или восстановление благополучия в разных типах семей.
АКСИОЛОГИЧЕСКАЯ ПАРАДИГМА КАК ОСНОВА ФОРМИРОВАНИЯ И СОХРАНЕНИЯ ЦЕННОСТНОЙ ОРИЕНТАЦИИ
Authors: Лугуценко Татьяна Валентиновна , Герасимов Алексей Вячеславович
Published: December 2024

Цель исследования заключается в рассмотрении аксиологической парадигмы как основы формирования и сохранения ценностной ориентации.
Методы исследования. Исследование основано на принципах отражения, понимания, конструирования, структурирования, системности, движения от отдельного к общему, от чувственного к рациональному, от абстрактного к конкретному; единства рефлексии и саморефлексии, дифференциации и интеграции, индукции и дедукции.
Результаты исследования. Аксиологическая парадигма как основа формирования и сохранения ценностной ориентации является неким образцом, а если использовать термин М. Вебера — идеальным типом, желательной системы ценностей. При этом было выяснено, что ценности отражаются в системе ценностных ориентаций, особенности которых обуславливают установки каждого человека. Полагаем, что именно явление социальной установки пронизывает все сферы индивидуального и общественного сознания. Итак, выявлено, что ценностные ориентации, представляющие собой системы установок на определенные ценности (в соответствии с Указом Президента Российской Федерации от 09.11.2022 г. №809 для российской цивилизации — это прежде всего традиционные духовно-нравственные ценности), являются основой, позволяющей осуществлять оценивание явлений бытия, а также выбирать соответствующие ориентиры в быстро трансформирующемся социуме. Таким образом, такие компоненты, как ценности, ценностные ориентации, идеалы, являются составляющими морали как системы высшего порядка, являющимися основой духовности личности. Итогом ценностных преобразований является появление духовно развитой личности, которая имеет соответствующие моральные способности.
Перспективы исследования. Результаты исследования могут быть использованы в междисциплинарной области, включающей вклад специалистов в области философии, социологии, психологии. Проблема рассмотрения аксиологической парадигмы как основы формирования и сохранения ценностной ориентации представляет как теоретический, так и практический интерес в связи с необходимостью понимания социальных рисков для дальнейшего развития российского социума.
ПРОБЛЕМА БАЛАНСА ВЛАСТЕОТНОШЕНИЙ В КОНТЕКСТЕ МОДЕРНИЗАЦИИ ИНСТИТУТА ГОСУДАРСТВА
Authors: Кутовой Данила Анатольевич
Published: December 2024

Цель исследования — социально-философское комплексное изучение проблемы поддержания баланса во властеотношениях в современном обществе и государстве в процессе интеграции в них информационно-коммуникационных технологий. Обсуждение потенциальных векторов развития системы властеотношений в современном социуме и преодоления потенциальных кризисов, вызванных дисбалансом в них.
Методология исследования. В процессе исследования использовался широкий инструментарий социальной и политической философии, включающий аксиологический и синергетический подходы, диалектический, системно-структурный и герменевтический методы. Проводимое исследование имеет междисциплинарный характер, основывается на положениях теории социального действия М. Вебера, элитарной теории Г. Моска, социологического подхода Н. М. Коркунова.
Результаты исследования. Показано, что формирующие общественные отношения в цифровом пространстве направлены на удовлетворение политических и социальных потребностей личности в общества, но также способны предоставлять симулякры их удовлетворения. В результате у личности может создаваться иллюзия самореализации, при этом в реальном социуме она становится дезорганизована. Установлено, что регуляция системы властеотношений в цифровом пространстве происходит путем опосредованного (сервисного) воздействия на них, при этом граждане в процессе самоорганизации преобразуют социум путем интеграции новых форм коммуникации в него. Цифровым платформам коммуникации нецелесообразно входить в конфронтацию с имеющимися политико-правовыми системами управления обществом. При этом современные технологии становятся инструментом поддержания баланса властеотношений, которые, несмотря на изменение их внутренней структуры, сохраняют свою властную природу, поскольку служат естественной потребности общества к самоорганизации и регуляции институциолизированной системой управления.
Перспективы исследования. Результаты исследования могут быть использованы для дальнейшей разработки проблемы властеотношений как перспективной формы раскрытия сущности влияния информационно-коммуникационных технологий на трансформацию социума.
ЦИФРОВИЗАЦИЯ СОВРЕМЕННОГО ВЫСШЕГО ОБРАЗОВАНИЯ В РОССИИ: ОСНОВНЫЕ ПРОБЛЕМЫ И ПЕРСПЕКТИВЫ
Authors: Колесина Карина Юрьевна
Published: December 2024

Целью исследования является определение основных проблем и перспективных направлений цифровизации системы высшего образования в современной России.
Методологическую базу составляют теоретические положения отечественных и зарубежных исследователей, раскрывающие характер и основное содержание процессов цифровизации высшего образования и описывающие основные проблемы и перспективы, связанные с применением цифровых технологий в высшей школе. В работе также применяются системный и диалектический подходы, позволившие исследовать цифровизацию высшего образования в условиях развития общества на современном этапе.
Результаты исследования. Цифровизация высшей школы осуществляется посредством внедрения в систему современного вузовского образования цифровых технологий и включает в качестве основных составляющих цифровизацию учебного процесса, инфраструктуры высших учебных заведений, организационных и управленческих элементов их функционирования. Цифровые технологии выступают в качестве одного из значимых инструментов, призванных способствовать повышению качества подготовки специалистов в высших учебных заведениях. В то же время цифровизация высшей школы имеет не только очевидные позитивные эффекты, но и характеризуется определенными угрозами и рисками: эффект от цифровизации может быть достигнут только при условии наличия высокого уровня цифровых навыков и компетенций у субъектов учебного процесса, достаточного уровня мотивации к использованию широких возможностей, предоставляемых электронными образовательными платформами, современного технического оснащения высших учебных заведений. Цифровые и другие средства не могут полностью заменить традиционные образовательные технологии, поэтому наиболее оптимальный вариант подготовки специалистов в высшей школе предусматривает симбиоз всех позитивных характеристик традиционных и цифровых методик вузовского образования.
Перспективу исследования составляет последующее научное изучение процессов цифровизации в системе современного российского высшего образования в комплексе с анализом проблем и перспектив, связанных с внедрением цифровых технологий в отечественных вузах.
A Convenient Analytic Method for Gel Quantification Using ImageJ Paired with Python or R. v1
Authors: Tomlinson Cassidy
Published: December 2024

This protocol details the convenient analytic method for gel quantification using ImageJ paired with Python or R.
JAX-Sen: Nuclei prep from frozen heart tissue for single-nuclei RNA-seq v1
Authors: L Daigle Sandra
Published: December 2024

We aim to study and characterize senescence in the C57BL/6J mouse heart. This protocol describes the nuclei prep from frozen heart tissue for single-nuclei RNA-seq.
Plasmid Construction Protocol v1
Authors: Ren Xuefeng , SI Cook Annan
Published: December 2024

This protocol details plasmid construction in general terms for the insertion of genes into the pCAG mammalian expression vector.
The educational change of Prussian military academy and Wilhelm II’s role
Authors:   The Korean Society For German History, Nho Hyung Suck
Published: December 2024

This study tried to examine what changes occurred in the education of Prussian regular schools and Prussian military academies after 1889, and what kind of role Wilhelm II played in it and how much influence he exercised. Through this, I tried to verify the correctness or wrongness of Kennedy's opinion and the theory of shadow emperor claimed by Wehler.
As revealed through this study, in order to prevent the expansion of socialism and mitigate excessive education, Wilhelm II exerted considerable influence over the Prussian Ministry of Education and the supervisory body of Prussian military academies, and changed the curriculum of elementary and secondary schools and military academies, especially led not only the educational changes in the Normal School, but also the changes in the number of educational hours in the military academies in the direction he wanted. Even not only the compilation of new textbooks at the military academies but also changes in the contents of German and history education were accomplished as he wanted.
Based on the above studies, it is clear that Wehler's view that Wilhelm II did not achieve personal rule and as a nominal supreme decision maker, he did not actually decide the policy of the empire, that is, traditional oligarchies combined with anonymous forces exercising authoritarian and pluralistic rule stamped it is an overgeneralization.
In addition, Wehler's argument that Wilhelm II was “at times” an “influential person” who used “personnel policy” to exert influence, but that he “exercised influence as a single actor alongside other actors on important decision-making processes” is less persuasive. On the contrary, Kennedy's view that Wilhelm II, the center of the policy-making body and the final decision-maker that favored individual rule, “had a comparatively greater role and influence” seems far more correct.
Die Ernährungspolitik und Biopolitik des Nazi-Regimes: Die Politik des Vollkornbrot-Programms im Fokus
Authors:   The Korean Society For German History, Sim Na Hye
Published: December 2024

Basierend auf den Erfahrungen der Deutschen während des Ersten Weltkriegs mit Hungerzuständen, ergriff das Nazi-Regime nach der Machtergreifung die Hauptpolitik der Selbstversorgung und -sicherung von Nahrungsmitteln. Die ideologische Feindseligkeit der Nazis gegenüber der Industrialisierung führte zu einer Begründung für die Steigerung der landwirtschaftlichen Produktivität als Vorbereitung auf den Zweiten Weltkrieg und den Anspruch auf Lebensraum. Natürlich wurden die Sicherung von Nahrungsmitteln und eine angemessene Verteilung zu wichtigen Mitteln, um den Kriegssieg und die Zukunft der Deutschen vorzubereiten. Die Politik des Vollkornbrot-Programms spielte dabei eine entscheidende Rolle. Als moderne politische Macht, die die Befugnis zur Lebensverwaltung der Bevölkerung übernahm, führte das Nazi-Regime eine Form der Biopolitik für das Leben der geschwächten Deutschen durch, ähnlich wie es zuvor eine Form der Lebenspolitik für die Juden zur Auslöschung verfolgt hatte. Symbolisches Essen dafür war das Vollkornbrot. Die weniger verarbeiteten, Vollkornbrote, die auf der medizinischen Tradition der Naturheilkunde basieren, die in der späten Hälfte des 19. Jahrhunderts entstand und die Heilkraft der Natur zur Genesung nutzt, wurden in Deutschland aufgrund ihrer symbolischen Bedeutung für Heilung und Stärke angenommen. Die Dunkelheit des Vollkornbrots, im Gegensatz zum weißen Brot, wurde auch als geeignetes Mittel verwendet, um nationale Feindseligkeiten gegenüber Frankreich, das als Symbol der Zivilisation galt, zu verstärken.
A Military-Historical Study of Post-War German Reconstruction: The July 20th Military Resistance and the Bundeswehr
Authors:   The Korean Society For German History, Lee Byong Chol
Published: December 2024

This paper sheds light on the post-war reconciliation and new beginnings in Germany after 1945, which is one of the most important subjects in modern German history, from the perspective of military history. Disarmed after the defeat in World War II, Germany began rearmament in response to the deepening Cold War and the needs of the Allied powers and Germany. However, the lack of alternative options in terms of personnel continuity with the Wehrmacht, the military of the Third Reich, limited Germany's new military departure. The premise was that there had to be some form of personnel continuity with the defense forces.
The new German military had to transform from a military of the Nazi regime to an integrated military in a democratic society. It had to be a military subordinate to politics, rather than being a “state within a state.” The foundation for this new attempt was already in place in German military history. It was the resistance that pursued a “different” Germany by defying the Nazis on July 20, 1944. Moreover, it was a resistance within the military leadership. Therefore, the new German military had a basis to begin anew on its own.
The situation of the post-war German military was characterized by a dualistic approach, aiming for a democratic military while maintaining personnel continuity with the defense forces. This paper examines how the Bundeswehr, established in 1955, developed under these conditions from the perspective of the acceptance of the July 20th resistance. It can be seen as a gradual process of development in which the Bundeswehr moved towards a progressive direction between the old and new traditions.
The Halved Constitutional State: The early FRG from the point of view of the past policy
Authors:   The Korean Society For German History, Lee Byong Chol
Published: December 2024

Today's critical discussion about the Nazi past is regarded as a national policy in Germany. However, there remains a void that still needs to be publicly addressed. The judicial handling of Nazi crimes, which is a key issue in Germany's dealing with its past, belongs to an area with significant gaps that need further elucidation in the general perception of the Third Reich and academic research. The indicator of this handling is the criminal prosecution of violent crimes by the totalitarian regime, namely mass murder. The problem is that the criminal prosecution, which is the first step in the judicial reckoning of the Nazi past, was delayed. In the early Federal Republic, where more emphasis was placed on reconstruction through integration than thorough denazification, the process of uncovering and punishing Nazi crimes started late and ended with results far from true reckoning with the past.
The delay in trials for Nazi crimes as judicial reckoning reflects the social and historical characteristics of the time. Due to personal continuity, the cohesion of the 'national community' established by the Third Reich persisted among the members of post-war German society. This meant that society was not ready to be outraged by the crimes committed by the illegal state, and the capacity for such outrage was minimal. Instead of a critical and unrelenting historical investigation under the deliberately propagated web of Nazism, social self-justification and subsequent self-exoneration were the main concerns. The result was 'belated prosecution.' The criminal prosecution of Nazi mass murders proceeded slowly, and the judgments were very lenient. The delay in judicial reckoning in the early Federal Republic hindered the development of post-war Germany into a constitutional state and democracy. It cannot be overlooked that the 'successful democracy' initially operated as a 'formal rule of law.' Considering the incomplete 'substantive rule of law,' the early Federal Republic is assessed as a 'halved constitutional state.'
Research Trends of Korean-German Relation History since 2001
Authors:   The Korean Society For German History, Jeong Daehoon , Thomas Eichert Thomas Eichert
Published: December 2024

This paper reviews the trends in the study of Korean-German relations published in Korean, German, and English since 2001. Since Joseon and Germany established diplomatic relations in 1883, Korean-German relations have continued for 140 years. In particular, after World War II, both countries formed a bond of sympathy by sharing the experience of forced division. This led to the early start of the study of Korean-German relations. If we limit ourselves to the year 2001, there are approximately 120 studies in Korean on Korean-German relations. Of these, about 80 are in German and English. This number is not insignificant.
The Korean-published research delves deeper than just presenting basic facts. It explores the perceptions and actions of Germans in Joseon (Korean Empire) during the modern period. It also expands the research scope to include diplomatic relations with Germany and democratization movements in relation to Korean political history. Recently, there has been an increased interest in economic history, including West German financial and technical assistance. Additionally, there is a growing focus on the activities and experiences of individual migrant workers, who were previously only considered as groups. The expansion and deepening of research on Korean-German relations may be directly related to improved accessibility to German materials.
However, research on the history of Korean-German relations in German and English is still limited in terms of topic diversity, despite the relatively large number of publications. Many of these studies are in the form of papers dealing with Korean-German relations, making it difficult to evaluate the level of activity in this field. Nonetheless, it is evident that the overall number of publications has increased in recent years. The rise in global interest in Korea and the growth of Korean studies in Germany may explain the increase in studies on Korean-German relations outside of Korea. It is expected that this trend will continue as universities in Germany continue to produce researchers and conduct related research.
A comprehensive review of monkeypox virus: Epidemiology, transmission, and control measures
Authors: Singel Harsh Vinubhai , Norris Saurabh Chhotalal , Pandya Himani Bhardwaj , Pipaliya Binda Prakashbhai , Javadekar Tanuja Bakul
Published: December 2024

Monkeypox virus (MPXV), a zoonotic pathogen related to smallpox, has gained attention due to its resurgence and potential for outbreaks. This review provides a comprehensive analysis of MPXV’s epidemiology, transmission, and control measures, focusing on major outbreaks in India, including recent cases in 2022 and 2023. The review discusses the viral structure, pathogenesis, and clinical presentations, emphasizing diagnostic challenges and outbreak management strategies. Key diagnostic methods such as PCR, viral culture, and serology are explored. The roles of diagnostic agencies in India, including the National Institute of Virology (NIV) Pune and the Indian Council of Medical Research (ICMR), are evaluated. The review highlights the importance of early detection, improved public health responses, and effective prevention strategies to mitigate future outbreaks. Recommendations for enhanced outbreak management and future research directions are also outlined.
Seroprevalence of SARS-CoV-2 IgG antibodies in health care workers and the associated risk factors during the covid-19 pandemic period at a tertiary care heatlh center in South India
Authors: Fatima Sarwat , Hazari Ahmed Abdul Bari , Handa Deepika
Published: December 2024

: Most of the published studies across the globe so far have demonstrated a highly variable seroprevalence rate of SARS-CoV-2 IgG antibodies in health care workers [HCW’s] during the pandemic period. Multiple risk factors were studied that influenced the seropositivity.

: The primary aim of the study was to determine the seroprevalence of SARS-CoV-2 IgG in HCW’s in local settings during the pandemic period. The secondary objective was to categorize the HCW’s based on the history of symptoms of covid-19 and also to evaluate the relation between the various risk factors and seropositivity amongst the HCW’s.

: A cross sectional prospective study was conducted on health care workers at a tertiary care hospital in Telangana state during the pandemic period between March to April 2021. Following enrollment and verbal consent from the participants descriptive data was collected using a validated questionnaire. SARS-CoV-2 IgG antibodies estimation was done using ELFA technology after obtaining IERB clearance.

: Of the 230 enrolled participants 141 [61.30%] fulfilled the inclusion criteria. The overall SARS-CoV-2 IgG seropositivity in the present study was 48 [34.04%]. Of the 72 [51.06%] symptomatic HCW’s, 19 [26.38%] were seropositive and of the 69 [48.93%] asymptomatic, 29 [42.02%] were seropositive. Among the various risk factors studied none were found to be significantly associated with seropositivity.

: in the present study a greater number of asymptomatic HCW’s were found seropositive which could be due to several of the reasons discussed below one being silent transmission of the virus and demands further enquiry into the cause by improved research methodology.
Recent updates in supratentorial ependymoma with approach to integrated diagnosis: Review
Authors: Jagtap Sunil Vitthalrao , Jagtap Shubham S , Ghadge Neha N , Chavan Vaibhav V , Jadhav Abhilasha S , Bandolkar Nishant D
Published: December 2024

Ependymomas are a distinct entity of glial tumor. We describe a patient with a supratentorial ependymoma with extensive calcification. A 28 year old male patient came with complaints of bilateral upper limb and lower limb weakness, head ache, accompanied by vertigo, vomiting, twice focal seizures over the past two to three months. Associated with lethargy and dizziness. Previous history of transient ischemic attack was there for which treatment was received. He had no history of hypertension, diabetes. On physical examination, spasticity present in both lower extremity and left unilateral grade II facial nerve palsy. Clinical diagnosis was give as right parietal space occupying lesion. CT, MRI brain showed well-defined heterogenously enhancing multilobulated, intra-axial lesion in right parietal lobe involving the post central gyrus with areas of calcification and necrosis. Features suggestive of primary malignant glial neoplasm. He underwent right craniotomy with excision of space occupying lesion. Histopathologically reported as Ependymomas with extensive intratumoral calcification. The tumour on immunostaining was positive for glial fibrillary acid protein (GFAP), Epithelial membrane antigen (EMA), and L1CAM was focally positive. The Ki-67 labelling index was less than 2%. Expression of p53 protein, IDH-1, and neuronal proteins such as synaptophysin and enolase was not observed. we report this case of supratentorial Ependymoma for its clinical, radioimaging, histopathological, immunohistochemical findings with recent updates and approach to integrated diagnosis. The ependymoma with extensive intratumoral calcification is rare finding.
Determinantes sociales de la salud y estado nutricional en la primera infancia indígena: Risaralda, Colombia
Authors: Pérez-Gómez María Alejandra , Vélez-Álvarez Consuelo
Published: December 2024

Introducción: los indígenas enfrentan problemas de salud derivados de persistentes desigualdades sociales. Objetivo: establecer los determinantes sociales de salud asociados con el estado nutricional de menores de 5 años de la comunidad Emberá Chamí de Mistrató, Risaralda. Materiales y métodos: estudio observacional de corte transversal. Se recopilaron datos de evaluación nutricional y determinantes sociales; las asociaciones se determinaron mediante análisis bivariado. Resultados: participaron 348 infantes, la mayoría entre 24 y 59 meses. El 72,4 % presentó desnutrición crónica y el 60,9 % riesgo de bajo peso para la edad y desnutrición global. En los signos físicos de malnutrición, el 17 % tuvo manchas grisáceas en dentina y el 7,8 % palidez facial. Se hallaron diferencias significativas (p < 0,05) entre la edad y los indicadores Peso/Talla, Talla/Edad, Peso/Edad y signos físicos, así como entre los ingresos económicos y Talla/Edad y Peso/Edad, con una fuerza de asociación positiva, y baja, pero mayor en Talla/Edad (0,198). El número de residentes en hogar se relacionó con Talla/Edad (p = 0,038) y perímetro cefálico (p = 0,023). La ocupación del padre se asoció con Peso/Edad (p = 0,003), y la lactancia materna total con Peso/Edad (p= 0,010) y signos físicos (p = 0,033). Se presentaron diferencias significativas entre el consumo de cereales, grasas, azúcares y leguminosas y el indicador Talla/Edad. La disposición de basuras se asoció con dicho indicador (p = 0,007), y la forma de consumo de agua con signos físicos (p = 0,000). En general, la fuerza de la asociación de los determinantes sociales de la salud con el estado nutricional fue positiva, aunque baja, y mayor con la edad del infante, escolaridad del padre, consumo de azúcares y disposición de basuras. Conclusión: los determinantes sociales asociados con el estado nutricional fueron edad, ingresos, escolaridad y ocupación de los padres, afiliación al sistema de seguridad, disposición de basuras, lactancia materna y consumo de alimentos (cereales, grasas, azúcares).
Nutritional value and antioxidant activity of ora-pro-nobis, tamarillo and green banana biomasses
Authors: Ferreira Renata de Souza , Santos Jucenir dos , Oliveira Lívya Alves de , Della Lucia Ceres Mattos , Vidigal Márcia Cristina Teixeira Ribeiro
Published: December 2024

The consumption of unconventional food plants (UFP) can be a strategy to promote food and nutritional security, for which it is necessary to know their nutritional composition. The objective of this study was to analyze the nutritional value and antioxidant activity of ora-pro-nobis, tamarillo and bananas biomass (prata and nanica). Using standard methodologies (AOAC, 2005), the following compounds were analyzed in triplicate: protein, lipids, carbohydrate, calories, moisture, total fiber content, calcium, iron, vitamin C (by titrimetry), carotenoids (by High Performance Liquid Chromatography), phenolic compounds (by Folin-Ciocalteau) and antioxidant activities, by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis 3-ethylbenzothiacholine-6-sulfonic acid). Ora-pro-nobis stands out as a source of calcium, iron, carotenoids and protein, is low in fat and has good antioxidant activity. Tamarillo showed high antioxidant activity, is a protein source and low in fat. The two banana biomasses had similar results in most nutrients, but the “nanica” banana with more protein and the “prata” with greater antioxidant activity. The unconventional food plants analyzed are potential bioactive foods, because they have phenolic compounds and high antioxidant activity. Thus, UFP have the potential to promote a more diverse and nutritious diet for consumers in general.
Naturaleza jurídica y control jurisdiccional de los actos de intervención monetaria proferidos por la Junta Directiva del banco central en Colombia
Authors: Galvis Macías Iván Humberto
Published: December 2024

Frente a los problemas de inflación que caracterizaron a la mayoría de países del mundo en la pospandemia, el presente trabajo tiene por objeto realizar un análisis de la naturaleza jurídica que ostentan los actos mediante los cuales la autoridad monetaria pretende controlar este fenómeno, vale decir, los actos de intervención monetaria, y, de esta manera, determinar mediante qué mecanismos es posible desarrollar su control jurisdiccional. Lo anterior se abordará a través de un enfoque cualitativo, analítico y descriptivo que, fundamentado en la revisión de fuentes jurídicas y sustentos doctrinarios, identificará la naturaleza y atribuciones del Banco de la República de Colombia y su Junta Directiva como autoridad autónoma e independiente y que ejerce sus funciones por medio de, por un lado, la aplicación de un concepto material de función administrativa, lo que hace que sus actos sean reconocidos como verdaderos actos administrativos de carácter general caracterizados, además, por su amplio margen de discrecionalidad; y, por el otro, mediante actos de naturaleza privada. Los primeros serán susceptibles de control jurisdiccional a través de los medios de control de nulidad por inconstitucionalidad y nulidad simple, en tanto que los segundos serán enjuiciables acudiendo a las correspondientes acciones ordinarias que se deriven del negocio jurídico celebrado.
A Comparative Immune Response of Pigs Vaccinated with and without Adjuvanted Cell Culture Adapted Classical Swine Fever Virus Vaccine
Authors: Suokhrie Nouluongunuo , Sarma Jayashree , Basumatary Firfila , Gogoi Sophia M. , Bharali Arpita , Buragohain Lukumoni , Barman Nagendra Nath
Published: December 2024

Background: Classical swine fever is a highly contagious economically devastating viral disease of pigs. In endemic country like India including NE States, vaccination is the best way for prevention and control. Although live attenuated vaccines are available, booster vaccination gives better protective immunity. Present study was designed to evaluate immunity and duration of immune response in cell culture CSF vaccine with and without incorporation of adjuvant. Methods: In the present study, four groups of CSFV free pigs containing 6 pigs in each group were immunized as follows- with cell culture adapted whole CSFV vaccine, adjuvanted with montanide oil, primary CSFV vaccine with booster and without booster and control group as unvaccinated. Immune response was demonstrated by per cent inhibition in blocking ELISA. Result: Vaccinated pigs elicited early antibody by 7th dpv which reached a peak at 30th dpv and maintaining high antibody titre upto 180dpv. Single oil adjuvanted whole vaccine effectively stimulated neutralizing antibody with 90-95% inhibition titre. In primary and booster vaccinated pigs, antibody titres showed significant difference. Oil adjuvanted vaccine was safe eliciting high neutralizing antibody titre with single vaccination till marketable age. Also a good comparison of all the four combinations of vaccines were obtained.
Molecular Cloning and Functional Analysis of MsDUF from Alfalfa (Medicago sativa L.)
Authors: Han Bo , Duan Xinhui , Jiang Hua , Zhao Yan , Wang Yafang , Hu Tianming , Yang Peizhi
Published: December 2024

Background: As a high-quality legume forage, Medicago sativa is restricted by various abiotic stresses during its growth and development. Proteins with domains of unknown function (DUF) are reportedly involved in abiotic stress tolerance in plants. However, the biological functions of DUF-containing proteins in alfalfa (Medicago sativa L.) related to environmental stress remain poorly understood. Methods: We reported previously a novel stress-responsive gene, MsDUF, from alfalfa that was up-regulated under drought stress. In this study, MsDUF, a 210 amino acid protein containing a DUF4228 domain, was identified in alfalfa. Furthermore, MsDUF-overexpressing tobacco plants were constructed to explore the functions of MsDUF in plant resistance to abiotic stress. Result: Compared with wild-type tobacco, relative water content, osmoregulator (proline and soluble sugars) content and superoxide dismutase activity were elevated and malondialdehyde content was decreased in leaves of MsDUF-overexpressing plants under drought and salt stress (p less than 0.05). The results indicate that MsDUF plays a vital role in responses to drought and salt stress in tobacco. Thus, this protein could be used to improve drought and salt resistance of tobacco and other crops.
ظواهر التدخل المفرداتي في مجموعة الوتساب "دروس اللغة" (دراسة تحليلية تقومية)
Authors: Arifah Nurdiana , Wulandari Novia
Published: December 2024

The existence of the WhatsApp group “Language Lessons” is as a wasilah to train Arabic language skills. In the process of communication trough the group, there was interference with mufrodat. So that in this study a descriptive qualitative method is used to analyze and describe the mufrodat error. The data source used in this study is the writing language in the whatsApp group “Language Lessons”. And in the data analysis, three stages are used, namely the data condensation stage, data presentation, and conclusion drawn. The focus of the research in this study is two, the first is how the interference phenomenon occurs in the Durusul Lughah WhatsApp Group. And the second is what is meant by the type of mufrodat error that occurs in the Durusul Lughah WhatsApp Group. Then the result of the focus of the problem is that the phenomenon of mufrodat fatigue often occur when they send a message through written language. And this type of mufrodat errors is caused by the lack of knowledge possessed by the speaker about mufrodat, and also due to the influence of the mother tongue.(WhatsApp grup “Durusul Lughah” yaitu adalah media untuk melatih kemampuan berbahasa arab. Komunikasi yang digunakan di grup ini menggunakan bahasa arab aktif, baik tulis maupun bicara. Sehingga peneliti menemukan interferensi bahasa saat anggota grup menulis pesan. Objek penelitian ini adalah tulisan pesan di WAG  “Durusul Lughah”. Penelitian ini menggunakan metode kualitatif deskriptif untuk menganalisis dan mendeskripsikan tentang interferensi mufrodat tersebut. Fokus penelitian dalam kajian ini yaitu ada dua, yang pertama bagaimana fenomena interferensi yang terjadi di WhatsApp grup “Durusul Lughah”. Dan yang kedua apa yang dimaksud dengan jenis kesalahan mufrodat yang terjadi di WhatsApp grup “Durusul Lughah”. Kemudian hasil dari fokus perasalahan tersebut bahwa fenomena kesalahan mufroat banyak terjadi ketika mereka mengirim sebuah pesan melalui bahasa tulisan. Dan jenis kesalahan mufrodat tersebut diakibatkan karena kurangnya pengetahuan yang dimiliki oleh penutur tentang mufrodat, dan juga diakibatkan oleh pengaruh dari bahasa ibu).
Neokolonialisme Lingkungan Hidup: Membongkar Narasi Penindasan dalam Karya Sastra Perspektif Postkolonial Ecocriticism Graham Huggan dan Helen Tiffin
Authors: Hidayani Vini
Published: December 2024

Neocolonialism in third-world countries persists in the form of human and environmental oppression. This oppression serves the capitalist agenda through large corporations. This research aims to expose the colonial narratives perpetuated by capitalists and states as reflected in Indonesian literary works. The formal framework adopted in this study is the perspective of Postcolonial Ecocriticism by Graham Huggan and Helen Tiffin, with the material objects of analysis being two literary works: "Tanggal Tujuh Belas Tanpa Tahun" by Triyanto Triwikromo and the short story "Teluk Nyomit" by Korrie Layun Rampan. The study seeks to elucidate the position of literature and its relationship with developmental discourse in modern society, and to examine its vulnerability to abuses of power wielded by state apparatus. Additionally, this article examines the faces of oppression faced by indigenous communities and the environment under capitalist systems. The research employs the Critical Discourse Analysis approach by Fairclough. The findings demonstrate that literature plays aesthetic, advocacy, and activist roles in unraveling oppression depicted in Indonesian literary works, portraying indigenous communities in both narratives as marginalized and displaced from their ancestral lands.(Neokolonialisasi di negara ketiga senantiasa terus berlangsung dalam bentuk penindasan manusia dan lingkungan. Bentuk penindasan ini mengarah ke tujuan kapitalisme melalui perusahaan-perusahaan besar. Penelitian ini hendak membongkar narasi kolonial yang dilakukan oleh pemilik modal dan negara yang termuat dalam karya sastra Indonesia. Objek formal yang digunakan dalam penelitian ini adalah perspektif Postcolonial Ecocriticism Graham Huggan dan Helen Tiffin, sedangkan objek materialnya menggunakan dua karya sastra, yang masing-masing berjudul “Tanggal Tujuh Belas Tanpa Tahun” karya Triyanto Triwikromo dan cerpen berjudul “Teluk Nyomit” karya Korrie Layun Rampan. Penelitian ini bertujuan untuk menjelaskan posisi sastra dan kaitannya dengan wacana pembangunan dalam masyarakat modern serta melihat keterpengaruhannya dari abuse of power yang digunakan oleh perangkat negara, selain itu, artikel ini juga hendak melihat wajah penindasan masyarakat adat dan lingkungan yang dilakukan oleh sistem kapitalisme. Metode penelitian yang digunakan adalah pendekatan Analisis Wacana Kritis Fairclouh. Hasil penelitian menunjukkan bahwa sastra memiliki peran estetika, advokasi, dan aktivisme dalam membongkas penindasan yang termuat dalam karya sastra Indonesia, dan masyarakat adat dalam kedua cerpen ditampilkan dalam potret yang tersisih dan terusir dari tanahnya sendiri.)
Evaluación por mérito genético de tilapias del Nilo (GIFT) en su segunda generación
Authors: Damas-Pérez Teresa de Jesus , Barrueta-López Daylen Virgen
Published: December 2024

The present study aimed to determine the genetic value of Nile tilapia GIFT in its second generation. The data evaluated corresponded to 19 families obtained from the genetic improvement program of tilapia in Cuba. An evaluation was performed on the entire population to determine each individual's genetic value, classifying it into high, medium, and low. The results obtained for high genetic merit were 362 and 322 grams for males and females, respectively, demonstrating the superiority of males over females for the live weight characteristic. The genetic parameters and variance components were estimated: additive, residual and phenotypic variance. As a fundamental element, heritability was estimated, characterized by being classified as high by obtaining a value of 0.49. The evaluation of genetic merit in genetic improvement programs represents a high potential to improve productivity through genetic selection.