bluuebunny/crossref_metadata_2025 huggingface dataset:
Microbial membrane transport proteins and their biotechnological applications
Authors: Özkan Melek , Yılmaz Hilal , Ergenekon Pınar , Erdoğan Esra Meşe , Erbakan Mustafa
Published: January 2024

Because of the hydrophobic nature of the membrane lipid bilayer, the majority of the hydrophilic solutes require special transportation mechanisms for passing through the cell membrane. Integral membrane transport proteins (MTPs), which belong to the Major Intrinsic Protein Family, facilitate the transport of these solutes across cell membranes. MTPs including aquaporins and carrier proteins are transmembrane proteins spanning across the cell membrane. The easy handling of microorganisms enabled the discovery of a remarkable number of transport proteins specific to different substances. It has been realized that these transporters have very important roles in the survival of microorganisms, their pathogenesis, and antimicrobial resistance. Astonishing features related to the solute specificity of these proteins have led to the acceleration of the research on the discovery of their properties and the development of innovative products in which these unique properties are used or imitated. Studies on microbial MTPs range from the discovery and characterization of a novel transporter protein to the mining and screening of them in a large transporter library for particular functions, from simulations and modeling of specific transporters to the preparation of biomimetic synthetic materials for different purposes such as biosensors or filtration membranes. This review presents recent discoveries on microbial membrane transport proteins and focuses especially on formate nitrite transport proteins and aquaporins, and advances in their biotechnological applications.
Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo-plate methods and monitoring of multiplication ability of regenerated shoots
Authors: Vujović Tatjana , Anđelić Tatjana , Marković Zvjezdana , Gajdošová Alena , Hunková Júlia
Published: January 2024

The purpose of this work was to apply V and D cryo-plate methods for cryopreservation of Vaccinium corymbosum ʻToroʼ, Fragaria × ananassa ʻCleryʼ, and Amelanchier alnifolia (Nutt.) M. Roem. and to monitor the multiplication capacity of shoots regenerated from cryopreserved explants. Shoot tips pre-cultured for 1 d at 23°C in the dark on medium containing 0.3 M sucrose were used as explants. Loading was performed in a solution containing 2 M glycerol and 0.8 M sucrose (30 min at room temperature). In the V cryo-plate, dehydration was carried out at room temperature (20 to 50 min) using the following plant vitrification solutions: original PVS2, 90% PVS2 solution, and PVS3. Regarding the D cryo-plate, dehydration was performed in closed glass containers over silica gel for 2, 2.5, or 3 h. In both protocols, rewarming was carried out in a 1.0 M sucrose solution (15 min at 25°C). Regenerated shoots were multiplied and multiplication parameters were monitored after the second subculture. Using the V cryo-plate method, the highest regrowth in highbush blueberry was obtained following 50-min treatment with all three VSs (61.7 to 80.9%). The D cryo-plate method was even more suitable with maximum regrowth of 89.4% achieved after 2.5 h of desiccation. For strawberry, 62.5% was the highest regrowth recorded using PVS3-based V cryo-plate method while 83.3% of regrowth was observed using D cryo-plate protocol. Regrowth of saskatoon reached a maximum of 50% after 50-min treatment with PVS3 while it did not exceed 40% in other treatments. By the second subculture, shoots regenerated from cryopreserved explants regained and even exceeded the multiplication capacity of shoots regenerated from non-cryopreserved explants. This study is the first to present the successful application of the V cryo-plate method in highbush blueberry, as well as the utilization of both V and D cryo-plate methods in saskatoon.
Upper partial sternal split for pediatric cardiac surgery
Authors: Shikata Fumiaki , Shah Jay , Marathe Supreet , Suna Jessica , Alphonso Nelson , Venugopal Prem
Published: January 2024

Objectives

We introduced the use of an upper partial sternal split for pediatric cardiac surgical procedures in our unit in 2016. We report the outcomes of our experience in 51 patients using this approach.

Methods

From February 2016 to September 2022, 51 patients underwent congenital cardiac surgical procedures using an upper partial sternal split including vascular ring repair (n = 20), subaortic membrane (n = 12), ventricular septal defect closure with aortic valve resuspension (n = 9), aortic arch repair (n = 4), pulmonary artery band (n = 2), pulmonary artery sling (n = 1), supravalvular aortic stenosis (n = 1), aortic valve replacement (n = 1), and pulmonary artery plasty (n = 1). The surgical approach involved a midline skin incision, based on the manubrium, followed by an upper manubriotomy. No special surgical instrumentation was required. Median patient age was 2.9 years (IQR 1.3, 6.0); median body weight was 15 kg (IQR 9.8, 20).

Results

There was no mortality and no patient required intraoperative conversion to full sternotomy. One patient required re-exploration for bleeding when the incision was converted to a full sternotomy. There were no wound complications in any patient. Twenty-one patients (41%) were extubated on the table and of the remaining 30 patients, 23 patients (76%) were extubated within 24 h of surgery. Eleven patients did not require intensive care unit (ICU) admission. Median ICU and hospital stay was 1 day (IQR 1, 1.25) and 5 days (IQR 4, 8) ,respectively.

Conclusion

An upper partial sternal split approach is straightforward and can be performed safely with a preferable cosmetic result in selected pediatric cardiac operations.
How the digital business ecosystem affects stakeholder satisfaction: its impact on business performance
Authors: Fernández-Portillo Antonio , Ramos-Vecino Nuria , Ramos-Mariño Adelaida , Cachón-Rodríguez Gabriel
Published: January 2024

Due to the digital transformation, organizations have incorporated technologies in all areas of the company, creating digital business ecosystems. These in turn, with the tools that compose it, influence the satisfaction of stakeholders and business performance. The objective of this research is to know how the digital business ecosystem affects customer and employee satisfaction and whether this satisfaction has an impact on business performance. To achieve this objective, a model is proposed to which a PLS technique is applied to a sample of 1319 Spanish SMEs. The results of this innovative study show, on the one hand, a positive relationship of the digital business ecosystem with the satisfaction of both stakeholders, and, on the other hand, and as the main contribution of this study, it has been found that employee satisfaction positively influences business performance. This research offers a novel model capable of relating how the satisfaction of both customers and employees in a digital environment improves business performance. It also contributes to the literature by widening the field of study and overcomes a new gap for SMEs.
Revising the core knowledge confusions scale: a measure of logical error associated with cognitive and personality traits
Authors: Williams Brenton M. , Browne Matthew , Rockloff Matthew , Stuart George
Published: January 2024

The Core Knowledge Confusions scale (CKC) was designed to predict paranormal and spiritual beliefs, alternative health beliefs and the degree to which people assign meaning to events. It measures the likelihood of individuals' tendency to accept ontologically confused content as literally true and has been used to investigate beliefs such as the paranormal. However, the psychometric properties of the CKC have not been tested, limiting the scale’s practical utility. The CKC was revised and tested, internal consistency was checked, and associations to paranormal belief were assessed using a panel survey of 1010 Australian residents (Mage = 52, SD = 17.69, Female = 56%). Using structural equation modelling and regression, the revised scale (CKC-R) content deviated from previous findings. However, it demonstrated acceptable internal, construct, and divergent validity. External correlates of the CKC-R were aligned with expectations: associating with high verbal knowledge, an intuitive cognitive style, and the absorption personality trait (i.e., a tendency to experience altered states of consciousness). The CKC-R provides researchers with a validated measure predicting paranormal belief that is associated with both cognitive and personality-based traits. Interpretation of the CKC-R as a measure of ontological error is less clear and requires further investigation.
Serum LDL Promotes Microglial Activation and Exacerbates Demyelinating Injury in Neuromyelitis Optica Spectrum Disorder
Authors: Chen Man , Chu Yun-Hui , Yu Wen-Xiang , You Yun-Fan , Tang Yue , Pang Xiao-Wei , Zhang Hang , Shang Ke , Deng Gang , Zhou Luo-Qi , Yang Sheng , Wang Wei , Xiao Jun , Tian Dai-Shi , Qin Chuan
Published: January 2024

Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune inflammatory demyelinating disease of the central nervous system (CNS) accompanied by blood-brain barrier (BBB) disruption. Dysfunction in microglial lipid metabolism is believed to be closely associated with the neuropathology of NMOSD. However, there is limited evidence on the functional relevance of circulating lipids in CNS demyelination, cellular metabolism, and microglial function. Here, we found that serum low-density lipoprotein (LDL) was positively correlated with markers of neurological damage in NMOSD patients. In addition, we demonstrated in a mouse model of NMOSD that LDL penetrates the CNS through the leaky BBB, directly activating microglia. This activation leads to excessive phagocytosis of myelin debris, inhibition of lipid metabolism, and increased glycolysis, ultimately exacerbating myelin damage. We also found that therapeutic interventions aimed at reducing circulating LDL effectively reversed the lipid metabolic dysfunction in microglia and mitigated the demyelinating injury in NMOSD. These findings shed light on the molecular and cellular mechanisms underlying the positive correlation between serum LDL and neurological damage, highlighting the potential therapeutic target for lowering circulating lipids to alleviate the acute demyelinating injury in NMOSD.
Interspecific social interaction between golden jackal (Canis aureus) and red fox (Vulpes vulpes)
Authors: Böcker Felix , Weber Hannah , Arnold Janosch , Collet Sebastian , Hatlauf Jennifer
Published: January 2024

In south-western Germany, a territorial single male golden jackal (Canis aureus) was repeatedly photographed showing social interaction with a red fox (Vulpes vulpes) female and her cubs. This unusual behaviour was documented in two subsequent years (August–September 2020 and May–August 2021). The interspecific actions are not limited to encounters of the two species but include interactions such as feeding and related sociopositive behaviours. Thirty-two observations with both species appearing together were recorded within the study period. The observed behaviour raises questions about the coexistence of both species and on interspecific behaviour of wild canids in general. Social isolation of the observed male golden jackal could be one of the potential drivers for the interaction, as Germany is at the current edge of golden jackal distribution in central Europe.
AM Bench 2022 Macroscale Tensile Challenge at Different Orientations (CHAL-AMB2022-04-MaTTO) and Summary of Predictions
Authors: Moser Newell , Benzing Jake , Kafka Orion L. , Weaver Jordan , Derimow Nicholas , Rentz Ross , Hrabe Nikolas
Published: January 2024

The additive manufacturing benchmarking challenge described in this work was aimed at the prediction of average stress–strain properties for tensile specimens that were excised from blocks of non-heat-treated IN625 manufactured by laser powder bed fusion. Two different laser scan strategies were considered: an X-only raster and an XY raster, which involved a 90$$^\circ $$

∘

rotation in the scan direction between subsequent layers. To measure anisotropy, multiple tensile orientations with respect to the build direction were investigated (e.g., parallel, perpendicular, and intervals in between). Benchmark participants were provided grain structure information via electron backscatter diffraction measurements, as well as the stress–strain response for tensile specimens manufactured parallel to the build direction and produced by the XY scan strategy. Then, participants were asked to predict tensile properties, like the ultimate tensile strength, for the remaining specimens and orientations. Interestingly, the measured mechanical properties did not vary linearly as a function of tensile orientation. Moreover, specimens manufactured with the XY scan strategy exhibited greater yield strength than those corresponding to the X-only scan strategy, regardless of orientation. The benchmark data have been made publicly available for anyone that is interested [1]. For the modeling aspect of the challenge, five teams participated in this benchmark. While most of the models incorporated a crystal plasticity framework, one team chose to use a more semiempirical approach and to great success. However, no team excelled at all the predictions, and all teams were seemingly challenged with the predictions associated with the X-only scan strategy.
Understanding the bonding mechanism in short-time resistance projection welding: a comprehensive analysis
Authors: Koal Johannes , Baumgarten Martin , Zschetzsche Jörg , Schmale Hans Christian
Published: January 2024

Capacitor discharge welding (CDW) is characterized by a pulsed electrical current profile. It is primarily utilized for resistance projection welding tasks, offering high power densities and short welding times. According to the latest findings, the welding process can be divided into different phases: contacting, activating, material connection, and holding pressure. During the activation phase, high-speed video-imaging reveals the generation of metal vapor which effectively eliminates impurities and oxide layers from the contact zone. The result of this is an activated surface. The purpose of this paper is to describe the physical effects of the bonding mechanism during short-time resistance welding. The Chair of Joining Technology and Assembly at the Technische Universität Dresden has a laboratory facility that can interrupt the welding current at any desired time during capacitor discharge welding. This allows different welding current profiles with always the same current rise time to be scientifically investigated. The experimental findings were supplemented with simulative analyses to clarify the bonding mechanism in resistance projection welding. Three different surface conditions are considered to generalize the findings on the bonding mechanism. Temperature and current density distributions were assessed to provide a physical description of the activation phase. The power density in the joining zone at different interruption times is determined, which gives an indication of activation by metal vaporization. The material connection is determined experimentally for the same interruption times. The numerical simulation model can be used to describe the bonding mechanism in short-time resistance welding. In resistance welding, the bond is formed due to the molten phase (solidification structure). In short-time resistance welding, the bond is formed due to surface activation by metal vaporization.
The influence of various grease compositions and silver nanoparticle additives on electrically induced rolling-element bearing damage
Authors: Bond Samuel , Jackson Robert L. , Mills German
Published: January 2024

Leakage currents accelerate surface degradation of metal contacts via small scale arcing across lubricating films, but recent observations suggest that metallic nanoparticle additives in lubricants may be useful to improve contact performance. These findings prompted a study that examined electrically induced surface pitting of steel contacts in the presence of several lubricating greases including some containing nanometer-sized colloidal silver (Ag) particles. Reciprocating rolling sphere-on-disk experiments were conducted under electro-tribological loads employing polyurea greases derived from mineral and synthetic base oils with and without additives. Friction forces and electrical resistance were monitored continuously during the tests; surface changes were characterized by means of optical spectroscopy, stylus profilometry, and scanning electron microscopy (SEM) including compositional analysis using energy dispersive spectroscopy (EDS). The observations demonstrate that surface pitting induced by arcing occurs mainly at the points were the rolling motion changes direction and that eroded metal is deposited along the wear grove. Micron-sized pits are formed which contain carbon and oxygen indicating that arcing causes decomposition of the hydrocarbon lubricants. Numerous findings indicate a significant inhibition of pitting is induced by the Ag nanoparticles; some greases containing other additives exhibit a similar, although less pronounced, effect.
Semen quality and metabolic profile in people with type 1 diabetes with and without erectile dysfunction: a cross-sectional study
Authors: Longo M. , Caruso P. , Varro C. , Tomasuolo M. , Cirillo P. , Scappaticcio L. , Romano L. , Arcaniolo D. , Maiorino M. I. , Bellastella G. , De Sio M. , Esposito K.
Published: January 2024

Purpose

The aim of the present study is to evaluate the association of metabolic and glycemic variables with semen parameters in patients with type 1 diabetes (T1D) with and without erectile dysfunction (ED).

Methods

The study population included 88 adults with T1D using a continuous glucose monitoring, of whom 28 with ED (ED group) and 60 without it (NO ED group). All men completed the International Index of Erectile Function (IIEF-5) and underwent body composition analysis (BIA) and semen analysis.

Results

ED group showed worse HbA1c levels [median (IQR), 8.4 (7.7, 9.9) vs 7.4 (7, 8.2) %, P < 0.001)], higher insulin dose [60 (51, 65) vs 45 (38, 56) UI/die, P = 0.004)] and a higher total body water and intracellular water as compared with ED group. Men in the ED group presented higher semen volume [2.8 (2.6, 4.2) vs 2.5 (2.2, 2.7) mL, P < 0.001] and sperm concentration [24 (19, 29) vs 20 (12, 23) mil/mL, P = 0.010], but reduced sperm progressive motility [28 (25, 35) vs 35 (25, 36) %, P = 0.011], higher rate of non-progressive motility [15 (10, 15) vs 10 (5, 10) %, P < 0.001] and higher rate of typical morphology [7(5, 8) vs 5 (4, 5) %, P = 0.001]. Based on multivariate logistic regression analysis performed to assess the association between clinical variables and ED, intracellular water (OR 3.829, 95% CI 1.205, 12.163, P = 0.023) resulted as the only independent predictor of ED.

Conclusion

Men with T1D and ED showed worse metabolic profile which is associated with poor semen quality, as compared with those without ED.
Three dimensional discrete element modelling of the conventional compression behavior of gas hydrate bearing coal
Authors: Gao Xia , Wang Nannan , Zhang Baoyong , Lin Qiqi , Wu Qiang , Meng Wei , Liu Xia
Published: January 2024

To analyze the relationship between macro and meso parameters of the gas hydrate bearing coal (GHBC) and to calibrate the meso-parameters, the numerical tests were conducted to simulate the laboratory triaxial compression tests by PFC3D, with the parallel bond model employed as the particle contact constitutive model. First, twenty simulation tests were conducted to quantify the relationship between the macro–meso parameters. Then, nine orthogonal simulation tests were performed using four meso-mechanical parameters in a three-level to evaluate the sensitivity of the meso-mechanical parameters. Furthermore, the calibration method of the meso-parameters were then proposed. Finally, the contact force chain, the contact force and the contact number were examined to investigate the saturation effect on the meso-mechanical behavior of GHBC. The results show that: (1) The elastic modulus linearly increases with the bonding stiffness ratio and the friction coefficient while exponentially increasing with the normal bonding strength and the bonding radius coefficient. The failure strength increases exponentially with the increase of the friction coefficient, the normal bonding strength and the bonding radius coefficient, and remains constant with the increase of bond stiffness ratio; (2) The friction coefficient and the bond radius coefficient are most sensitive to the elastic modulus and the failure strength; (3) The number of the force chains, the contact force, and the bond strength between particles will increase with the increase of the hydrate saturation, which leads to the larger failure strength.
Reproductive Outcomes of Conventional In Vitro Fertilization and Intracytoplasmic Sperm Injection in Patients with Non-Severe Male Infertility Across Poor and Different Sub-Optimal Ovarian Response Categories: A Cohort Study Based on 30,352 Fresh Cycles from 2009–2019
Authors: Jiesisibieke Dina , Tian Tian , Zhu Xiaxuan , Fang Shilin , Zhang Nan , Ma Jinxi , Xia Yuqi , Li Rong , Liu Ping , Qiao Jie , Yang Rui
Published: January 2024

Due to the influence of economic, social and many other factors, there are more and more reproductive problems. Originally introduced for managing male factor infertility, intracytoplasmic sperm injection had become the most commonly used fertilization treatment in the world, with broadened indications including low oocyte yield, prior fertilization failure with conventional in vitro fertilization etc. However, academic evidence for better live-birth outcomes of intracytoplasmic sperm injection over conventional in vitro fertilization is limited. Thus, we aimed to compare the reproductive outcomes of conventional in vitro fertilization and intracytoplasmic sperm injection in patients with non-severe male factor infertility across poor and different sub-optimal ovarian response categories. The fertility rate, implantation rate, clinical pregnancy rate, live birth rate and other obstetric outcomes were mainly compared. Our results showed that independent of the number of oocytes retrieved, intracytoplasmic sperm injection significantly increased the fertilization rate, while conventional in vitro fertilization cycles showed a higher implantation rate, clinical pregnancy rate, and live birth rate. No differences were observed in most obstetric outcomes. Our study indicates that poor ovarian response is not an indication for intracytoplasmic sperm injection in couples with non-severe male infertility.
Aromatase inhibition using Juniperus procera phytochemical constituents: molecular docking study
Authors: Al-Zahrani Ateeq Ahmed
Published: January 2024

The key step in the biosynthesis of estrogen is the enzyme activity of aromatase. Several malignancies, including breast cancer, have been linked to the initiation and progression of estrogen overexpression. Exemestane, Arimidex and Femara are the most common aromatase inhibitors used to treat hormone-dependent breast cancers. Drug resistance and side effects are commonly associated with these treatments. The purpose of this in silico study was to list the chemical compounds of Juniperus procera that have been published in scientific papers. The second goal was to evaluate the inhibitory activity of 124 phytochemicals of Juniperus procera compared to known aromatase inhibitors such as Exemestane, Arimidex and Femara. The 3D structure of aromatase (PDB id: 3s7s) employed for docking studies using AutoDock Tools as well as normal mode analysis studies utilizing the NMSim web server. Juniperolide, Kaurenoic acid and Isocupressic acid were identified as competitive aromatase inhibitors compared to FDA approved anti-cancer drugs, specifically Exemestane, Arimidex and Femara. The stability of the ligand–protein interface was studied to support the docking findings. To our knowledge, this is the first study that investigates the possible inhibition roles of some compounds of Juniperus procera on the aromatase enzyme.
Scientific Models and Decision Making
Authors: Winsberg Eric , Harvard Stephanie
Published: January 2024

This Element introduces the philosophical literature on models, with an emphasis on normative considerations relevant to models for decision-making. Chapter 1 gives an overview of core questions in the philosophy of modeling. Chapter 2 examines the concept of model adequacy for purpose, using three examples of models from the atmospheric sciences to describe how this sort of adequacy is determined in practice. Chapter 3 explores the significance of using models that are not adequate for purpose, including the purpose of informing public decisions. Chapter 4 provides a basic framework for values in modelling, using a case study to highlight the ethical challenges in building models for decision making. It concludes by establishing the need for strategies to manage value judgments in modelling, including the potential for public participation in the process.
Transcendental Epistemology
Authors: Cheng Tony
Published: January 2024

Transcendental arguments were prominent in Western philosophy, German idealism, phenomenological tradition, and P. F. Strawson's thinking. They have fallen out of fashion because of their associations with transcendental idealism and verificationism. They are still invoked by important figures in the analytic tradition even if the very same tradition has cast doubt on such arguments. The nature of transcendental arguments remains unclear: Are they supposed to be deductive? Are they synthetic or analytic? If they are a priori, how are they supposed to be about the empirical world? What are their relations to necessity, conceivability, and essence? This Element takes up the challenge of elucidating the nature of transcendental arguments, embedded in the wider context of transcendental epistemology. It will be argued that the key premise 'transcendental conditional' is synthetic, necessary, and a posteriori.
Approaches to Spread, Scale-Up, and Sustainability
Authors: Papoutsi Chrysanthi , Greenhalgh Trisha , Marjanovic Sonja
Published: January 2024

Few interventions that succeed in improving healthcare locally end up becoming spread and sustained more widely. This indicates that we need to think differently about spreading improvements in practice. Drawing on a focused review of academic and grey literature, the authors outline how spread, scale-up, and sustainability have been defined and operationalised, highlighting areas of ambiguity and contention. Following an overview of relevant frameworks and models, they focus on three specific approaches and unpack their theoretical assumptions and practical implications: the Dynamic Sustainability Framework, the 3S (structure, strategy, supports) infrastructure approach for scale-up, and the NASSS (non-adoption, abandonment, and challenges to scale-up, spread, and sustainability) framework. Key points are illustrated through empirical case narratives and the Element concludes with actionable learning for those engaged in improvement activities and for researchers. This title is also available as Open Access on Cambridge Core.
Linear and nonlinear receptivity mechanisms in boundary layers subject to free-stream turbulence
Authors: Blanco Diego C.P. , Hanifi Ardeshir , Henningson Dan S. , Cavalieri André V.G.
Published: January 2024

Large-eddy simulations of a flat-plate boundary layer, without a leading edge, subject to multiple levels of incoming free-stream turbulence are considered in the present work. Within an input–output model, where nonlinear terms of the incompressible Navier–Stokes equations are treated as an external forcing, we manage to separate inputs related to perturbations coming through the intake of the numerical domain, whose evolution represents a linear mechanism, and the volumetric nonlinear forcing due to triadic interactions. With these, we perform the full reconstruction of the statistics of the flow, as measured in the simulations, to quantify pairs of wavenumbers and frequencies more affected by either linear or nonlinear receptivity mechanisms. Inside the boundary layer, different wavenumbers at near-zero frequency reveal streaky structures. Those that are amplified predominantly via linear interactions with the incoming vorticity occur upstream and display transient growth, while those generated by the nonlinear forcing are the most energetic and appear in more downstream positions. The latter feature vortices growing proportionally to the laminar boundary layer thickness, along with a velocity profile that agrees with the optimal amplification obtained by linear transient growth theory. The numerical approach presented is general and could potentially be extended to any simulation for which receptivity to incoming perturbations needs to be assessed.
Commentary on ‘Institutional Logics: Motivating Action and Overcoming Resistance to Change’
Authors: Redding Gordon
Published: January 2024

This commentary applauds Haveman, Joseph-Goteiner, and Li's (2023) efforts to build arguments around institutional logics to explain China’s remarkable economic progress since 1978. But it also calls for broadening the focus of this inquiry so it can more generally explain why and how some societies are able to build connections between cultures and institutions that enable widespread societal progress, while other countries fail to do so. In particular, I suggest that this line of inquiry would benefit from drawing more deeply on the extensive body of writings by economic historians who have compared the economic progress of different societies over a long period.
Milk fat globule membrane: formation and transformation
Authors: Wooding F. B. Peter , Kinoshita Masanao
Published: January 2024

The milk fat globule membrane (MFGM) is formed by complex cell biological processes in the lactating mammary epithelial cell which result in the release of the milk fat globule (MFG) into the secretory alveolus. The MFG is bounded by a continuous unit membrane (UM), separated from the MFG lipid by a thin layer of cytoplasm. This unique apocrine secretion process has been shown in all of the mammary species so far investigated. Once the MFG is released into the alveolus there is a considerable transformation of the UM with its attached cytoplasm. This is the MFGM. The transformation is stable and expressed milk shows the same transformed MFGM structure. Again, this transformation of structure is common to all mammalian species so far investigated. However, the explanation of the transformation very much depends on the method of investigation. Transmission electron microscope (TEM) studies suggest a literal breakdown to a discontinuous UM plus cytoplasm in patches and strands, whereas more recent confocal laser scanning light microscopy (CLSM) studies indicate a separation, in a continuous UM, of two phases, one liquid ordered and the other liquid disordered. This review is designed to show that the TEM and CLSM results show different views of the same structures once certain deficiencies in techniques are factored in.
The Decreased Cloud Cover Dominated the Rapid Spring Temperature Rise in Arid Central Asia Over the Period 1980–2014
Authors: Wang Gongxin , Yuan Xiuliang , Jing Changqing , Hamdi Rafiq , Ochege Friday Uchenna , Dong Ping , Shao Yuqing , Qin Xueyan
Published: January 2024

Central Asia (CA) has experienced a faster temperature rise than the global land over the past decades. However, the role of regional/global drivers and their associated underlying biophysical mechanisms is poorly explored. Here, we combined observations and model simulations to show that the rapid warming in CA was overwhelmingly contributed by rapid spring warming (i.e., 49.23%). The decrease of cloud cover (CLD) was the main driver of spring warming in CA, leading to the surface receiving more solar radiation, consequently heating the surface air temperature, and contributing almost 40.79% to the spring warming. Besides, the strengthening of sea level pressure states results in continuous subsidence of vertical motion over CA, which was unfavorable for cloud formation. Our study will deepen our understanding of the climate evolution in the arid CA.
Multi‐Proxy Evidence for Atlantic Meridional Overturning Circulation (AMOC) Weakening During Deglaciations of the Past 150,000 Years
Authors: Garity Monica , Lund David
Published: January 2024

Despite decades of research, the cause of deglaciations is not fully understood, leaving a critical gap in our understanding of Earth's climate system. During the most recent deglaciation (Termination I (T I)), abrupt declines in the stable carbon isotope ratio (δ13C) of benthic foraminifera occurred throughout the mid‐depth (1,500–2,500 m) Atlantic. The spatial pattern in δ13C anomalies was likely due to Atlantic Meridional Overturning Circulation (AMOC) weakening and the accumulation of respired carbon, which also yields negative excursions in carbonate ion concentration (). To investigate whether a similar pattern occurred during prior deglaciations, we developed δ13C and records from 1,800 and 2,300 m water depth in the Southwest Atlantic spanning the last 150 ka. The new records reveal negative δ13C and anomalies during Termination II (TII) and the smaller deglaciations of Marine Isotope Stages (MIS) 4/3, 5b/a, and 5d/c, suggesting AMOC weakening is a common feature of deglaciation. The anomalies are more pronounced in the shallower core following MIS 2, 4, and 6 and in the deeper core following MIS 5b and 5d. The depth‐dependent pattern is most likely due to shoaling of Northern Source Water during glacial maxima and deepening during interglacial intervals. Comparison of records from TI and TII suggests similar levels of carbon accumulation in the mid‐depth Atlantic. The Brazil Margin δ13C and results indicate the AMOC plays a key role in the series of events causing deglaciation, regardless of differences in orbital configuration, ice volume, and mean global temperature.
An ancient polymorphic regulatory region within the BDNF gene associated with obesity modulates anxiety-like behaviour in mice and humans
Authors: McEwan Andrew R. , Hing Benjamin , Erickson Johanna C. , Hutchings Greg , Urama Charity , Norton-Hughes Emily , D’Ippolito Mariam , Berry Susan , Delibegovic Mirela , Grassmann Felix , MacKenzie Alasdair
Published: January 2024

Obesity and anxiety are morbidities notable for their increased impact on society during the recent COVID-19 pandemic. Understanding the mechanisms governing susceptibility to these conditions will increase our quality of life and resilience to future pandemics. In the current study, we explored the function of a highly conserved regulatory region (BE5.1) within the BDNF gene that harbours a polymorphism strongly associated with obesity (rs10767664; p = 4.69 × 10–26). Analysis in primary cells suggested that the major T-allele of BE5.1 was an enhancer, whereas the obesity-associated A-allele was not. However, CRISPR/CAS9 deletion of BE5.1 from the mouse genome (BE5.1KO) produced no significant effect on the expression of BDNF transcripts in the hypothalamus, no change in weight gain after 28 days and only a marginally significant increase in food intake. Nevertheless, transcripts were significantly increased in the amygdala of female mice and elevated zero maze and marble-burying tests demonstrated a significant increase in anxiety-like behaviour that could be reversed by diazepam. Consistent with these observations, human GWAS cohort analysis demonstrated a significant association between rs10767664 and anxiousness in human populations. Intriguingly, interrogation of the human GTEx eQTL database demonstrated no effect on BDNF mRNA levels associated with rs10767664 but a highly significant effect on BDNF-antisense (BDNF-AS) gene expression and splicing. The subsequent observation that deletion of BE5.1 also significantly reduced BDNF-AS expression in mice suggests a novel mechanism in the regulation of BDNF expression common to mice and humans, which contributes to the modulation of mood and anxiety in both species.
ABLIM1, a novel ubiquitin E3 ligase, promotes growth and metastasis of colorectal cancer through targeting IĸBα ubiquitination and activating NF-ĸB signaling
Authors: He Ying , Shi Qian , Ling Yuhang , Guo Huihui , Fei Yi , Wu Ruoyu , Tang Chengwu , Zhang Xilin , Yao Linhua
Published: January 2024

Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, has been reported as a suppressor in several tumors whereas its role in colorectal cancer (CRC) remains unknown. In this study, we find that ABLIM1 is up-regulated in CRC patients and high levels of ABLIM1 predict short disease-free survival time. Knock-down of ABLIM1 in CRC cell lines by lenti-virus leads to inhibited cell proliferation, migration, and invasion capabilities in vitro and impaired growth of tumor xenografts and liver metastasis lesions in vivo, while ABLIM1 overexpression accelerates tumor growth and invasion in vitro. Mechanistically, we uncover that ABLIM1 activates the NF-ĸB/CCL-20 signaling through modulating IĸBα ubiquitination and proteasomal-mediated degradation. Further co-immunoprecipitation, in vivo and in vitro ubiquitination assays reveal ABLIM1 as a novel ubiquitin E3 ligase binding to IĸBα. Interestingly, The E3 ligase catalysis activity of ABLIM1 depends on its 402–778aa rather than its LIM domains and its interaction with IĸBα relies on the HP domain. Our findings delineate the oncogenic role of ABLIM1 in CRC progression and reveal it as a novel E3 ligase targeting IĸBα, providing new insights into the regulation of NF-ĸB signaling in tumors.
Recent progress in photoinduced transitions between the solid, glass, and liquid states based on molecular photoswitches
Authors: Imato Keiichi , Kaneda Naoki , Ooyama Yousuke
Published: January 2024

Photoinduced transitions between the solid, glass, and liquid states based on molecular photoswitches promise a wide variety of applications. Photoswitchable adhesives are representative examples and are expected to contribute to material recycling for a sustainable future in the era of composite materials due to strong bonding and on-demand photo-induced debonding with minimal damage to the adherends. Only a few molecular photoswitches are known to undergo these transitions, but recent progress, mainly with azobenzene, has been remarkable. Here, we review the photoinduced transitions of small molecules and polymers over approximately a decade and systematically discuss the molecular designs, mechanisms, applications, merits and demerits, and future challenges in each photoswitch and the whole field. We hope this review provides useful information, inspiration, and ideas for the development of this field and the expansion of its applications.
Chiral arylsulfinylamides as reagents for visible light-mediated asymmetric alkene aminoarylations
Authors: Hervieu Cédric , Kirillova Mariia S. , Hu Yawen , Cuesta-Galisteo Sergio , Merino Estíbaliz , Nevado Cristina
Published: January 2024

Two- or one-electron-mediated difunctionalizations of internal alkenes represent straightforward approaches to assemble molecular complexity by the simultaneous formation of two contiguous Csp3 stereocentres. Although racemic versions have been extensively explored, asymmetric variants, especially those involving open-shell C-centred radical species, are much more limited both in number and scope. Here we describe enantioenriched arylsulfinylamides as all-in-one reagents for the efficient asymmetric, intermolecular aminoarylation of alkenes. Under mild photoredox conditions, nitrogen addition of the arylsulfinylamide onto the double bond, followed by 1,4-translocation of the aromatic ring, produce, in a single operation, the corresponding aminoarylation adducts in enantiomerically enriched form. The sulfinyl group acts here as a traceless chiral auxiliary, as it is eliminated in situ under the mild reaction conditions. Optically pure β,β-diarylethylamines, aryl-α,β-ethylenediamines and α-aryl-β-aminoalcohols, prominent motifs in pharmaceuticals, bioactive natural products and ligands for transition metals, are thereby accessible with excellent levels of regio-, relative and absolute stereocontrol.
Programmable Heisenberg interactions between Floquet qubits
Authors: Nguyen Long B. , Kim Yosep , Hashim Akel , Goss Noah , Marinelli Brian , Bhandari Bibek , Das Debmalya , Naik Ravi K. , Kreikebaum John Mark , Jordan Andrew N. , Santiago David I. , Siddiqi Irfan
Published: January 2024

The trade-off between robustness and tunability is a central challenge in the pursuit of quantum simulation and fault-tolerant quantum computation. In particular, quantum architectures are often designed to achieve high coherence at the expense of tunability. Many current qubit designs have fixed energy levels and consequently limited types of controllable interactions. Here by adiabatically transforming fixed-frequency superconducting circuits into modifiable Floquet qubits, we demonstrate an XXZ Heisenberg interaction with fully adjustable anisotropy. This interaction model can act as the primitive for an expressive set of quantum operations, but is also the basis for quantum simulations of spin systems. To illustrate the robustness and versatility of our Floquet protocol, we tailor the Heisenberg Hamiltonian and implement two-qubit iSWAP, CZ and SWAP gates with good estimated fidelities. In addition, we implement a Heisenberg interaction between higher energy levels and employ it to construct a three-qubit CCZ gate, also with a competitive fidelity. Our protocol applies to multiple fixed-frequency high-coherence platforms, providing a collection of interactions for high-performance quantum information processing. It also establishes the potential of the Floquet framework as a tool for exploring quantum electrodynamics and optimal control.
Hourly values of an advanced human-biometeorological index for diverse populations from 1991 to 2020 in Greece
Authors: Giannaros Christos , Agathangelidis Ilias , Galanaki Elissavet , Cartalis Constantinos , Kotroni Vassiliki , Lagouvardos Konstantinos , Giannaros Theodore M. , Matzarakis Andreas
Published: January 2024

Existing assessments of the thermal-related impact of the environment on humans are often limited by the use of data that are not representative of the population exposure and/or not consider a human centred approach. Here, we combine high resolution regional retrospective analysis (reanalysis), population data and human energy balance modelling, in order to produce a human thermal bioclimate dataset capable of addressing the above limitations. The dataset consists of hourly, population-weighted values of an advanced human-biometeorological index, namely the modified physiologically equivalent temperature (mPET), at fine-scale administrative level and for 10 different population groups. It also includes the main environmental drivers of mPET at the same spatiotemporal resolution, covering the period from 1991 to 2020. The study area is Greece, but the provided code allows for the ease replication of the dataset in countries included in the domains of the climate reanalysis and population data, which focus over Europe. Thus, the presented data and code can be exploited for human-biometeorological and environmental epidemiological studies in the European continent.
Micro computed tomography images of capillary actions in rough and irregular granular materials
Authors: Nadimi Sadegh , Mendes Joao , López Alejandro , Schröer Laurenz , Manoorkar Sojwal , Ellman Sharon , Cnudde Veerle , Bruno Agostino Walter
Published: January 2024

The present work investigates the effect of both surface roughness and particle morphology on the retention behaviour of granular materials via X-ray micro-computed tomography (µCT) observations. X-ray µCT images were taken on two types of spherical glass beads (i.e. smooth and rough) and two different sands (i.e. natural and roughened). Each sample was subjected to drainage and soaking paths consisting in a multiphase ‘static’ flow of potassium iodine (KI) brine (wetting phase) and dry air (non-wetting phase). Tomograms were obtained at different saturation states ranging from fully brine saturated to air dry conditions with 6.2 μm voxel size resolution. The data acquisition and pre-processing are here described while all data, a total of 48 tomograms, are made publicly available. The combined dataset offers new opportunities to study the influence of surface roughness and particle morphology on capillary actions as well as supporting validation of pore-scale models of multiphase flow in granular materials.
Intimate partner violence during pregnancy and risks of low birth weight and preterm birth in hospitals of Tigray, Northern Ethiopia
Authors: Gebreslasie Kahsay Zenebe , Weldemariam Solomon , Gebre Gelawdyos , Zenebe Dawit , Mehari Mihreteab , Birhane Aklil , Dewey Rebecca Susan
Published: January 2024

Intimate partner Violence (IPV) can affect any woman, irrespective of their economic status, religion, or culture. This is a human-rights issue and due to its prevalence and adverse effects on pregnancy and birth, it must be given greater attention. Further, there is a lack of data in the Tigray region about adverse birth outcomes due to intimate partner violence during pregnancy. The aim of this study was to assess intimate partner violence during pregnancy and its association with low birth weight and preterm birth in Tigray region. Across-sectional study design was used. 647 women were involved in the study. Simple random sampling techniques were employed to select health facilities and systematic sampling was used to select study participants. Data were entered using Epi info version 3.5.1 and was analyzed using SPSSversion 20. Logistic regression analysis was conducted to assess the association between exposure to intimate partner violence during pregnancy and preterm birth and low birth weight while adjusting for possible confounders. The prevalence of intimate partner violence during pregnancy was 7.3% and the prevalence of low birth weight and preterm birth were 18.5% and 10.8% respectively. There was a statistically significant association between exposure to intimate partner violence during pregnancy and low birth weight. After adjustment for socioeconomic status, women’s habits and obstetric factors, the pregnant women who were exposed to intimate partner violence during pregnancy were two times more likely to have a child with a low birth weight (2.39 (95% CI: 1.26–4.55)). The prevalence of intimate partner violence during pregnancy, low birth weight, and preterm birth in this study was high. Women who experienced intimate partner violence during pregnancy had an increased risk of low birth weight. These findings justify a call to the federal minster of health to take measures aimed at avoiding intimate partner violence during pregnancy to reduce adverse birth outcomes.