bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancement of Amorphous Silica Dissolution by Interaction with Six-Membered Ring Heterocyclic Compounds
Authors: Kawano Motoharu , Hwang Jinyeon
Published: January 2020

Six-membered ring heterocyclic compounds are widely present in the Earth's surface environments as biological organic molecules composed of soil organic matter including plant and microbial residues, while little is known about their effect on the dissolution of silicate minerals including amorphous silica. To evaluate the effect of these biological molecules on amorphous silica dissolution, dissolution experiments were carried out by the flow-through method using 0.1 g of amorphous silica and 0.1 mM NaCl electrolyte solutions containing 0.0, 0.1, 1.0, or 10.0 mM of the heterocyclic compounds, piperidine (pK = 11.12), pyridine (pK = 5.25), or pyridazine (pK = 2.33), at a pH of 6, 5, or 4. Additionally, adsorption experiments of the compounds on the amorphous silica surface were performed to confirm the adsorption affinity for the amorphous silica surface. The results demonstrated that these heterocyclic compounds enhance the dissolution rate of amorphous silica in the following order: piperidine > pyridine > pyridazine. When 10.0 mM solutions were used, the heterocyclic compounds enhanced greatly the dissolution rate up to enhancement factors of 6.0 to ~14.8, 5.0 to ~14.0, and 1.0 to ~2.6 through an interaction of piperidine, pyridine, and pyridazine, respectively, in the pH range of approximately 6 to ~ 4. The adsorption experiments indicated that the heterocyclic compounds exhibited significant adsorption affinity for the amorphous silica surface as follows: piperidine > pyridine > pyridazine, which was consistent with the order of their effects on the dissolution enhancement. The geochemical calculation revealed that this order of enhancement was in good agreement with the concentrations of cationic species of heterocyclic compounds at corresponding pH conditions. Consequently, the enhancement of amorphous silica dissolution is likely to be influenced by the electrostatic complexation of the cationic species of the heterocyclic compounds with the negative >SiO– sites on the amorphous silica surface.
Penetrating Thoracic Injury and Fatal Aortic Transection From the Barb of a Stingray
Authors: Lau Hong Khai , Chua Ivan Si Yong , Ponampalam R.
Published: January 2020

Stingrays are found in open waters and are also kept in exhibits in many aquariums throughout the world. They are generally nonaggressive creatures by nature, but they can inflict injuries with their spines if provoked. We present a case of a 62-y-old diver who was pierced in the chest by the barb of a stingray while transferring the animal to another tank as part of his work in a public aquarium. He was rescued immediately from the tank but was found to be in cardiac arrest. Bystander cardiopulmonary resuscitation was promptly initiated by his colleagues. He was rapidly evacuated to the nearest emergency department, where he was noted to be in pulseless electrical activity. A single puncture wound was noted over the right second intercostal space, with the spine of the stingray still impaled in the chest. Trauma surgeons were activated promptly, and resuscitation was continued based on advanced cardiac and trauma life support guidelines, which included ongoing cardiopulmonary resuscitation, securing the airway, and emergency blood transfusion. An emergency department thoracotomy was performed, but despite aggressive resuscitation the thoracic injury was fatal. An autopsy revealed transection of the aorta by an impaled barb. We present a review of stingray injuries and suggest a general approach to management.
Sea-ice freeboard and thickness in the Ross Sea from airborne (IceBridge 2013) and satellite (ICESat 2003–2008) observations
Authors: Tian Liuxi , Xie Hongjie , Ackley Stephen F. , Tang Jiakui , Mestas-Nuñez Alberto M. , Wang Xianwei
Published: January 2020

NASA's Operation IceBridge mission flew over the Ross Sea, Antarctica (20 and 27 November 2013) and collected data with Airborne Topographic Mapper (ATM) and Digital Mapping System (DMS). Using the DMS and reflectivity of ATM L1B, leads are detected to define local sea level height. The total freeboard is then obtained and converted to ice thickness. The estimated mean sea-ice thickness values are found to be in the 0.48–0.99 m range. Along the N-S track, sea ice was thinner southward rather than northward of the fluxgate, resulting in two peaks of modal thickness: 0.35 m (south) and 0.7 m (north). This supports that new ice produced in coastal polynyas is transported northward by katabatic winds off the ice-shelf. The lowest (2%) elevation method used for freeboard retrieval for ICESat is also tested for ATM data. It is found that the lowest elevation method tends to overestimate freeboard, but mean values are less affected than mode values. Using mean thickness values of ICESat and ATM along the ‘fluxgate’, separating the shelf from the deep ocean, the exported ice volume at this ‘fluxgate’ is found to be higher during the ICESat years (2003–2008) than during the IceBridge year (2013).
‘Are we losing touch?’ Mainstream parties' failure to represent their voters on immigration and its electoral consequences
Authors: Brady David W. , Ferejohn John A. , Paparo Aldo
Published: January 2020

In many advanced democracies, mainstream political parties have been disrupted either by the rise of new (populist) parties or by hostile takeovers. In this article we argue that immigration attitudes have had a powerful impact on the strategic environment of political parties and leaders. We show, based on evidence from a comparative study conducted by YouGov in spring of 2015, that immigration attitudes had, by that time, driven a wedge between mainstream parties – those that regularly play a role in government – and their partisans. This ‘immigration gap’ opened up enormous space for new political movements to form, either inside existing parties or outside. Furthermore, we show that the representation gap on immigration issues is a relevant predictor of vote choice, so that parties are particularly likely to lose votes when they are more distant from their supporters on immigration.
The association between milk consumption and the metabolic syndrome: a cross-sectional study of the residents of Suzhou, China and a meta-analysis
Authors: Hidayat Khemayanto , Yu Lu-Gang , Yang Jin-Rong , Zhang Xue-Ying , Zhou Hui , Shi Yu-Jie , Liu Biao , Qin Li-Qiang
Published: January 2020

The association between milk consumption and the metabolic syndrome remains inconclusive, and data from Chinese populations are scarce. We conducted a cross-sectional study to investigate the association between milk consumption and the metabolic syndrome and its components among the residents of Suzhou Industrial Park, Suzhou, China. A total of 5149 participants were included in the final analysis. A logistic regression model was applied to estimate the OR and 95 % CI for the prevalence of the metabolic syndrome and its components according to milk consumption. In addition, the results of our study were further meta-analysed with other published observational studies to quantify the association between the highestv. lowest categories of milk consumption and the metabolic syndrome and its components. There was no significant difference in the odds of having the metabolic syndrome between milk consumers and non-milk consumers (OR 0·86, 95 % CI 0·73, 1·01). However, milk consumers had lower odds of having elevated waist circumference (OR 0·78, 95 % CI 0·67, 0·92), elevated TAG (OR 0·83, 95 % CI 0·70, 0·99) and elevated blood pressure (OR 0·85, 95 % CI 0·73, 0·99). When the results were pooled together with other published studies, higher milk consumption was inversely associated with the risk of the metabolic syndrome (relative risk 0·80, 95 % CI 0·72, 0·88) and its components (except elevated fasting blood glucose); however, these results should be treated with caution as high heterogeneity was observed. In summary, the currently available evidence from observational studies suggests that higher milk consumption may be inversely associated with the metabolic syndrome.
Daily intake of non-fried potato does not affect markers of glycaemia and is associated with better diet quality compared with refined grains: a randomised, crossover study in healthy adults
Authors: Johnston E. A. , Petersen K. S. , Kris-Etherton P. M.
Published: January 2020

Epidemiological studies suggest that consumption of potatoes is associated with increased risk of cardiometabolic diseases. However, few clinical trials have empirically tested this. The aim of this single-blind, randomised, crossover study was to evaluate the effect of daily potato consumption, compared with refined grains, on risk factors for cardiometabolic diseases. It was hypothesised that no difference in cardiometabolic endpoints would be detected between conditions, but diet quality would improve with potato consumption. Healthy participants on self-selected diets received one potato-based side dish or one refined grain-based side dish daily, for 4 weeks, separated by a minimum 2-week break. Dishes were isoenergetic, carbohydrate-matched and prepared without excess saturated fat or Na. Participants were instructed to consume the side dish with a meal in place of carbohydrates habitually consumed. Lipids/lipoproteins, markers of glycaemic control, blood pressure, weight and pulse wave velocity were measured at baseline and condition endpoints. Diet quality was calculated, based on 24-h recalls, using the Healthy Eating Index (HEI)-2015. Fifty adults (female n 34; age 40 (sd 13) years; BMI 24·5 (sd 3·6) kg/m2) completed the present study. No between-condition differences were detected for fasting plasma glucose (–0·05 mmol/l, 95 % CI –0·14, 0·04; P = 0·15), the primary outcome or any other outcomes. Compared with refined grains, the HEI-2015 score (3·5, 95 % CI 0·6, 6·4; P = 0·01), K (547 mg, 95 % CI 331, 764, P < 0·001) and fibre (2·4 g, 95 % CI 0·6, 4·2, P = 0·01) were higher following the potato condition. Consuming non-fried potatoes resulted in higher diet quality, K and fibre intake, without adversely affecting cardiometabolic risk.
Partial replacement of nitrogen fertilization with legumes in tropical pasture overseeded with temperate species for the production of steers
Authors: Schmitz G. R. , Paris W. , Biesek R. R. , Costa O. A. D. , Mafioletti R. D. , Umezaki A. M. , Menezes L. F. G.
Published: January 2020

Using legumes in forage mixes can help decrease the use of nitrogen fertilizers and possibly increase the nutritive value of pasture. The aim of the current study was to determine animal production and behavioural and ingestion parameters by evaluating the production and nutritive value of Aruana grass (Panicum maximum‘Aruana’) intercropped with forage peanut (Arachis pintoi‘Amarillo’) or fertilized with nitrogen. The treatments were N200 (200 kg N/ha per season – summer and winter), 100N + PE (100 kg N/ha per season plus pasture mixture with forage peanut) and N100 (100 kg N/ha per season). The presence of forage peanut in the pasture did not replace nitrogen fertilization as the pasture fertilized with 200 kg of nitrogen had a greater leaf/stem ratio (0.66v.0.54), stocking rate (2600v.2290 kg live weight/ha), average daily gain (0.880v.0.700 kg/day) and live weight gain (LWG) (652v.468 kg/ha) during summer. During winter, no effect of increased nitrogen fertilization on pasture and animal production was observed. In the total study period (summer + winter), a greater LWG (897v.741 kg/ha) occurred when a higher quantity of nitrogen (N200) was placed in the pasture compared to the insertion of forage peanuts in the system.
Assessing diet in European populations using national dietary surveys
Authors: Rippin Holly L. , Hutchinson Jayne , Jewell Jo , Breda Joao J. , Cade Janet E.
Published: January 2020

The WHO encourages countries to conduct national dietary surveys (NDS) to inform preventative policies targeting malnutrition and noncommunicable diseases. Previous reviews have found inadequate nutrient intakes and survey provision across Europe. This research is the first to provide an updated review of NDS provision within the whole WHO European Region, across the lifecourse, with reference to disadvantaged groups, obesity and nutrients of concern. Over a third of WHO European countries, mainly Central and Eastern European countries (CEEC), had no identifiable NDS. Where countries reported nutrient intakes, poor WHO recommended nutrient intake attainment was Europe-wide across the lifecourse, particularly in CEEC. Lower educated individuals had poorer diet quality. However, heterogeneity in age group sampled, dietary assessment method, nutrient composition database and under-reporting hindered inter-country comparisons. Average population trans fatty acid intakes below WHO recommended limits may hide inequalities in disadvantaged groups; legislative bans may help alleviate this. There were few associations between NDS-derived consumed food portion size (FPS) and BMI. However, consumed FPS was greater than on-pack serving-size in the majority of foods studied. This review illustrates how NDS can generate information on diet, nutrient intakes and the food environment. However, to enable valid inter-country comparisons, countries should be encouraged to conduct and report harmonised NDS, particularly in the age groups sampled, dietary assessment methodology, nutrient range, underpinning food composition database and treatment of under-reporters. This will aid effective, coordinated policy development that can have a real impact on dietary improvement, on a population and subgroup level, throughout Europe.
Spatial dynamics and activity patterns of the fosa Cryptoprocta ferox in Ankarafantsika National Park, Madagascar: carnivores navigating a human-influenced landscape
Authors: Wyza Eileen , Dollar Luke , Rahajanirina Leon Pierrot , Popescu Viorel , Stevens Nancy J.
Published: January 2020

The Vulnerable fosa Cryptoprocta ferox is the largest native carnivore in Madagascar, fulfilling a unique ecological niche in the island's remaining forests. Negative interactions with humans threaten the long-term viability of most remaining fosa populations across Madagascar. Threats to the fosa include habitat loss and persecution by humans resulting from perceived predation on domestic animals. We used GPS collars to record space use and activity patterns of five fosas in Ankarafantsika National Park, Madagascar, during the dry seasons of 2016 and 2017. The results, with up to 2,110 recorded locations per individual, indicated fosas’ home ranges and movements were not limited to the forest, and all collared individuals used networks of habitat patches and corridors to navigate deforested areas. The fosas studied in Ankarafantsika National Park had significantly larger home ranges than those reported in previous studies in other protected areas. They were rarely found within village boundaries and appeared to avoid areas of human habitation, suggesting that during the study period livestock was not a significant component of the fosas’ diet in this Park. Our results suggest that fosas have some flexibility that enables them to adapt to living near deforested and human-dominated areas by altering their space-use patterns, but they are compensating by increasing their home range size.
Schistosomiasis then and now: what has changed in the last 100 years?
Authors: Wilson R. Alan
Published: January 2020

Only with the completion of the life cycles of Fasciola hepatica in 1883 and 30 years later those of Schistosoma japonicum (1913), Schistosoma haematobium and Schistosoma mansoni (1915) did research on schistosomiasis really get underway. One of the first papers by Cawston in 1918, describing attempts to establish the means of transmission of S. haematobium in Natal, South Africa, forms the historical perspective against which to judge where we are now. Molecular biology techniques have produced a much better definition of the complexity of the schistosome species and their snail hosts, but also revealed the extent of hybridization between human and animal schistosomes that may impact on parasite adaptability. While diagnostics have greatly improved, the ability to detect single worm pair infections routinely, still falls short of its goal. The introduction of praziquantel ~1982 has revolutionized the treatment of infected individuals and led directly to the mass drug administration programmes. In turn, the severe pathological consequences of high worm burdens have been minimized, and for S. haematobium infections the incidence of associated squamous cell carcinoma has been reduced. In comparison, the development of effective vaccines has yet to come to fruition. The elimination of schistosomiasis japonica from Japan shows what is possible, using multiple lines of approach, but the clear and present danger is that the whole edifice of schistosome control is balanced on the monotherapy of praziquantel, and the development of drug resistance could topple that.
Introduction: special issue on the economics of climate change and sustainability (Part B)
Authors: Agliardi Elettra , Casari Marco , Xepapadeas Anastasios
Published: January 2020

Climate change is considered to be one of the most significant and complex challenges facing the world in the twenty-first century. As such, it is essential to increase the knowledge base regarding climate change and how best to address its impacts through efficient policies. This special issue, which is divided into two parts – published in the previous and current issues of Environment and Development Economics – is a selection of papers related to ‘The Economics of Climate Change and Sustainability’, the topic of an international workshop organized by the Economics Department of the University of Bologna in April 2018. The papers in this special issue cover a wide range of climate-change-related topics, including endogenous growth and overlapping generation models; climate-related financing and green bonds; demography; location decisions and technology diffusion; quantitative relationships and experimental approaches. They aim at providing new insights into the economics of climate change and help to identify new directions for future research.
Trainee self-assessment of cognitive behaviour therapy competence during and after training
Authors: Beale Sarah , Liness Sheena , Hirsch Colette R.
Published: January 2020

Large-scale cognitive behavioural therapy (CBT) training and implementation programmes, such as the pioneering Improving Access to Psychological Therapies (IAPT) initiative in the UK, aim to develop a workforce of competent therapists who can deliver evidence-based interventions skilfully. Self-awareness of competence enables CBT therapists to accurately evaluate their clinical practice and determine professional development needs. The accuracy of self-assessed competence, however, remains unclear when compared with assessments conducted by markers with expertise in CBT practice and evaluation. This study investigated the relationship between self- and expert-rated competence – assessed via therapy recordings rated on the Cognitive Therapy Scale Revised (CTS-R) scale – for a large sample of IAPT CBT trainees during training and, for the first time, at post-training follow-up. CBT trainees (n = 150) submitted therapy recordings at baseline, mid-training and end-of-training. At 12+ month follow-up, a subset of former trainees (n = 30) submitted recordings from clinical practice. There were positive relationships (r = .27 to .56) between self and expert CTS-R scores at all time points. The proportion of tapes demonstrating significant agreement between self and expert ratings (CTS-R difference <5 points) increased significantly across training and remained stable at follow-up. Findings indicate that accurate self-awareness of competence can be developed during structured CBT training and retained in the workplace. These outcomes are encouraging given the importance of self-awareness to CBT practice and accreditation. Future investigation into the development and maintenance of accurate self-awareness of competence is warranted.

Key learning aims

(1)

What is the relationship between self-ratings and expert ratings of CBT competence during training and at post-training follow-up?

(2)

Does agreement between self and expert competence ratings improve with CBT training?

(3)

How does agreement between self and expert ratings change across training for more- and less-competent trainees?

(4)

Can accurate self-awareness of competence be retained post-training in the workplace?
Cluster Observations on Time‐of‐Flight Effect of Oxygen Ions in Magnetotail Reconnection Exhaust Region
Authors: Wu T. , Fu S. Y. , Xie L. , Zong Q.‐G. , Zhou X. Z. , Yue C. , Sun W. J. , Pu Z. Y. , Xiong Y. , Zhao S. J. , Zhang H. , Yu F. B.
Published: January 2020

The D‐shaped ion velocity distribution generated by the time‐of‐flight (ToF) effect can be considered as an important characteristic of reconnection exhausts in the magnetopause and magnetotail reconnection processes. In this study, we reported the D‐shaped velocity distribution of O+ ions produced by the ToF effect in the magnetotail reconnection exhaust based on the observations from the Cluster spacecraft. The observed cutoff velocity of O+ ions is smaller than that of H+ ions at the same time, which is different from the previous theoretical prediction. We suggested that the difference between the two cutoff velocities is probably due to O+ ions having a larger reconnection diffusion region than H+ ions. We also demonstrated a remote‐sensing method to estimate the spatial scale ratio between the O+ and H+ diffusion regions and the distance from the observation site to the center of the magnetotail reconnection region.
Deformation of the Baige Landslide, Tibet, China, Revealed Through the Integration of Cross‐Platform ALOS/PALSAR‐1 and ALOS/PALSAR‐2 SAR Observations
Authors: Liu Xiaojie , Zhao Chaoying , Zhang Qin , Lu Zhong , Li Zhenhong
Published: January 2020

Twenty‐eight ALOS/PALSAR‐1 and ALOS/PALSAR‐2 images acquired from January 2007 to August 2018 were exploited to characterize the deformation history and temporal evolution of the Baige landslide, China, which successively occurred on 11 October and 3 November 2018. To mitigate the errors caused by the topographic relief and to conduct offset estimates between the SAR images from different platforms, a novel offset‐tracking method is proposed. The SAR images were ortho‐rectified firstly. Then two‐dimensional deformations were retrieved successfully from SAR images acquired at an identical platform and different platforms. Our results indicate that the maximum cumulative deformation in the line‐of‐sight direction of the Baige landslide reached about −60 m between January 2007 and August 2018. Furthermore, correlation between time series deformation and monthly precipitation and soil moisture derived from SAR intensity images suggests that the failure of the Baige landslide was closely related to the heavy rainfall in the summer of 2018.
Mycelial network-mediated rhizobial dispersal enhances legume nodulation
Authors: Zhang Wei , Li Xiao-Gang , Sun Kai , Tang Meng-Jun , Xu Fang-Ji , Zhang Ming , Dai Chuan-Chao
Published: January 2020

The access of rhizobia to legume host is a prerequisite for nodulation. Rhizobia are poorly motile in soil, while filamentous fungi are known to grow extensively across soil pores. Since root exudates-driven bacterial chemotaxis cannot explain rhizobial long-distance dispersal, mycelia could constitute ideal dispersal networks to help rhizobial enrichment in the legume rhizosphere from bulk soil. Thus, we hypothesized that mycelia networks act as vectors that enable contact between rhizobia and legume and influence subsequent nodulation. By developing a soil microcosm system, we found that a facultatively biotrophic fungus, Phomopsis liquidambaris, helps rhizobial migration from bulk soil to the peanut (Arachis hypogaea) rhizosphere and, hence, triggers peanut–rhizobium nodulation but not seen in the absence of mycelia. Assays of dispersal modes suggested that cell proliferation and motility mediated rhizobial dispersal along mycelia, and fungal exudates might contribute to this process. Furthermore, transcriptomic analysis indicated that genes associated with the cell division, chemosensory system, flagellum biosynthesis, and motility were regulated by Ph. liquidambaris, thus accounting for the detected rhizobial dispersal along hyphae. Our results indicate that rhizobia use mycelia as dispersal networks that migrate to legume rhizosphere and trigger nodulation. This work highlights the importance of mycelial network-based bacterial dispersal in legume–rhizobium symbiosis.
Rabbit ATG/ATLG in preventing graft-versus-host disease after allogeneic stem cell transplantation: consensus-based recommendations by an international expert panel
Authors: Bonifazi Francesca , Rubio Marie-Thérèse , Bacigalupo Andrea , Boelens Jaap Jan , Finke Jürgen , Greinix Hildegard , Mohty Mohamad , Nagler Arnon , Passweg Jakob , Rambaldi Alessandro , Socie Gérard , Solano Carlos , Walker Irwin , Barosi Giovanni , Kröger Nicolaus
Published: January 2020

This collaborative initiative aimed to provide recommendations on the use of polyclonal antithymocyte globulin (ATG) or anti-T lymphocyte globulin (ATLG) for the prevention of graft-versus-host disease (GvHD) after allogeneic hematopoietic stem cell transplantation (HSCT). A comprehensive review of articles released up to October, 2018 was performed as a source of scientific evidence. Fourteen clinically relevant key questions to the domains indication, administration, and post-transplant management were developed and recommendations were produced using the Delphi technique involving a Panel of 14 experts. ATG/ATLG was strongly recommended as part of myeloablative conditioning regimen prior to matched or mismatched unrelated bone marrow or peripheral blood allogeneic HSCT in malignant diseases to prevent severe acute and chronic GvHD. ATG/ATLG was also recommended prior to HLA-identical sibling peripheral HSCT with good but lesser bulk of evidence. In reduced intensity or nonmyeloablative conditioning regimens, ATG/ATLG was deemed appropriate to reduce the incidence of acute and chronic GvHD, but a higher risk of relapse should be taken into account. Recommendations regarding dose, application, and premedication were also provided as well as post-transplant infectious prophylaxis and vaccination. Overall, these recommendations can be used for a proper and safe application of polyclonal ATG/ATLG to prevent GvHD after allogeneic HSCT.
Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses
Authors: Hutchinson Luke D. , Darling Nicola J. , Nicolaou Stephanos , Gori Ilaria , Squair Daniel R. , Cohen Philip , Hill Caroline S. , Sapkota Gopal P.
Published: January 2020

The signalling pathways initiated by members of the transforming growth factor-β (TGFβ) family of cytokines control many metazoan cellular processes, including proliferation and differentiation, epithelial–mesenchymal transition (EMT) and apoptosis. TGFβ signalling is therefore strictly regulated to ensure appropriate context-dependent physiological responses. In an attempt to identify novel regulatory components of the TGFβ signalling pathway, we performed a pharmacological screen by using a cell line engineered to report the endogenous transcription of the TGFβ-responsive target gene PAI-1. The screen revealed that small molecule inhibitors of salt-inducible kinases (SIKs) attenuate TGFβ-mediated transcription of PAI-1 without affecting receptor-mediated SMAD phosphorylation, SMAD complex formation or nuclear translocation. We provide evidence that genetic inactivation of SIK isoforms also attenuates TGFβ-dependent transcriptional responses. Pharmacological inhibition of SIKs by using multiple small-molecule inhibitors potentiated apoptotic cell death induced by TGFβ stimulation. Our data therefore provide evidence for a novel function of SIKs in modulating TGFβ-mediated transcriptional and cellular responses.
Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis
Authors: Fu Rong , Lv Wen-Cong , Xu Ying , Gong Mu-Yun , Chen Xiao-Jie , Jiang Nan , Xu Yan , Yao Qing-Qiang , Di Lei , Lu Tao , Wang Li-Ming , Mo Ran , Wu Zhao-Qiu
Published: January 2020

Recent interest in the control of bone metabolism has focused on a specialized subset of CD31hiendomucinhi vessels, which are reported to couple angiogenesis with osteogenesis. However, the underlying mechanisms that link these processes together remain largely undefined. Here we show that the zinc-finger transcription factor ZEB1 is predominantly expressed in CD31hiendomucinhi endothelium in human and mouse bone. Endothelial cell-specific deletion of ZEB1 in mice impairs CD31hiendomucinhi vessel formation in the bone, resulting in reduced osteogenesis. Mechanistically, ZEB1 deletion reduces histone acetylation on Dll4 and Notch1 promoters, thereby epigenetically suppressing Notch signaling, a critical pathway that controls bone angiogenesis and osteogenesis. ZEB1 expression in skeletal endothelium declines in osteoporotic mice and humans. Administration of Zeb1-packaged liposomes in osteoporotic mice restores impaired Notch activity in skeletal endothelium, thereby promoting angiogenesis-dependent osteogenesis and ameliorating bone loss. Pharmacological reversal of the low ZEB1/Notch signaling may exert therapeutic benefit in osteoporotic patients by promoting angiogenesis-dependent bone formation.
Prenatal dietary supplements influence the infant airway microbiota in a randomized factorial clinical trial
Authors: Hjelmsø Mathis H. , Shah Shiraz A. , Thorsen Jonathan , Rasmussen Morten , Vestergaard Gisle , Mortensen Martin S. , Brejnrod Asker , Brix Susanne , Chawes Bo , Bønnelykke Klaus , Sørensen Søren J. , Stokholm Jakob , Bisgaard Hans
Published: January 2020

Maternal dietary interventions during pregnancy with fish oil and high dose vitamin D have been shown to reduce the incidence of asthma and wheeze in offspring, potentially through microbial effects in pregnancy or early childhood. Here we analyze the bacterial compositions in longitudinal samples from 695 pregnant women and their children according to intervention group in a nested, factorial, double-blind, placebo-controlled, randomized trial of n-3 long-chain fatty acids and vitamin D supplementation. The dietary interventions affect the infant airways, but not the infant fecal or maternal vaginal microbiota. Changes in overall beta diversity are observed, which in turn associates with a change in immune mediator profile. In addition, airway microbial maturation and the relative abundance of specific bacterial genera are altered. Furthermore, mediation analysis reveals the changed airway microbiota to be a minor and non-significant mediator of the protective effect of the dietary interventions on risk of asthma. Our results demonstrate the potential of prenatal dietary supplements as manipulators of the early airway bacterial colonization.
Persistent activation of central amygdala CRF neurons helps drive the immediate fear extinction deficit
Authors: Jo Yong S. , Namboodiri Vijay Mohan K. , Stuber Garret D. , Zweifel Larry S.
Published: January 2020

Fear extinction is an active learning process whereby previously established conditioned responses to a conditioned stimulus are suppressed. Paradoxically, when extinction training is performed immediately following fear acquisition, the extinction memory is weakened. Here, we demonstrate that corticotrophin-releasing factor (CRF)-expressing neurons in the central amygdala (CeA) antagonize the extinction memory following immediate extinction training. CeA-CRF neurons transition from responding to the unconditioned stimulus to the conditioned stimulus during the acquisition of a fear memory that persists during immediate extinction training, but diminishes during delayed extinction training. Inhibition of CeA-CRF neurons during immediate extinction training is sufficient to promote enhanced extinction memories, and activation of these neurons following delay extinction training is sufficient to reinstate a previously extinguished fear memory. These results demonstrate CeA-CRF neurons are an important substrate for the persistence of fear and have broad implications for the neural basis of persistent negative affective behavioral states.
Giant virus diversity and host interactions through global metagenomics
Authors: Schulz Frederik , Roux Simon , Paez-Espino David , Jungbluth Sean , Walsh David A. , Denef Vincent J. , McMahon Katherine D. , Konstantinidis Konstantinos T. , Eloe-Fadrosh Emiley A. , Kyrpides Nikos C. , Woyke Tanja
Published: January 2020

Our current knowledge about nucleocytoplasmic large DNA viruses (NCLDVs) is largely derived from viral isolates that are co-cultivated with protists and algae. Here we reconstructed 2,074 NCLDV genomes from sampling sites across the globe by building on the rapidly increasing amount of publicly available metagenome data. This led to an 11-fold increase in phylogenetic diversity and a parallel 10-fold expansion in functional diversity. Analysis of 58,023 major capsid proteins from large and giant viruses using metagenomic data revealed the global distribution patterns and cosmopolitan nature of these viruses. The discovered viral genomes encoded a wide range of proteins with putative roles in photosynthesis and diverse substrate transport processes, indicating that host reprogramming is probably a common strategy in the NCLDVs. Furthermore, inferences of horizontal gene transfer connected viral lineages to diverse eukaryotic hosts. We anticipate that the global diversity of NCLDVs that we describe here will establish giant viruses—which are associated with most major eukaryotic lineages—as important players in ecosystems across Earth’s biomes.
The Effect of a Substrate Material on Composition Gradients of Fe-Ni Films obtained by Electrodeposition
Authors: Białostocka Anna Maria , Klekotka Urszula , Kalska-Szostko Beata
Published: January 2020

The electrodeposition of FeNi alloy films was performed galvanostatically in the sulfate solution (Fe2+/Ni2+ mass ratio 1:2) in order investigate their co-deposition mechanism. The FeNi layers were deposited at variable substrates (copper, brass, silver) under the same chemical (electrolyte composition) and electric plating (current density value) conditions. After applying various time, substrates and external magnetic field orientation, the quality of the obtained film was examined. The surface morphology and crystallographic texture variation were investigated by the Scanning Electron Microscope (SEM), Energy Dispersive X-ray Spectroscopy (EDX), Wavelength Dispersive X-ray Fluorescence (WDXRF) and Laser Confocal Scanning Microscope (LCSM). The anomalous co-deposition of iron group metals is evidently dependent on the substrate.
Experimental Realization of Zenneck Type Wave-based Non-Radiative, Non-Coupled Wireless Power Transmission
Authors: Oruganti Sai Kiran , Liu Feifei , Paul Dipra , Liu Jun , Malik Jagannath , Feng Ke , Kim Haksun , Liang Yuming , Thundat Thomas , Bien Franklin
Published: January 2020

A decade ago, non-radiative wireless power transmission re-emerged as a promising alternative to deliver electrical power to devices where a physical wiring proved impracticable. However, conventional “coupling-based” approaches face performance issues when multiple devices are involved, as they are restricted by factors like coupling and external environments. Zenneck waves are excited at interfaces, like surface plasmons and have the potential to deliver electrical power to devices placed on a conducting surface. Here, we demonstrate, efficient and long range delivery of electrical power by exciting non-radiative waves over metal surfaces to multiple loads. Our modeling and simulation using Maxwell’s equation with proper boundary conditions shows Zenneck type behavior for the excited waves and are in excellent agreement with experimental results. In conclusion, we physically realize a radically different class of power transfer system, based on a wave, whose existence has been fiercely debated for over a century.
Efficacy of pregabalin for the treatment of chronic pruritus of unknown origin, assessed based on electric current perception threshold
Authors: Lee JaeIn , Jang DongHyek , Bae JooYoon , Jung HyeJung , Park MiYoun , Ahn JiYoung
Published: January 2020

Chronic pruritus of unknown origin (CPUO) is defined as itching lasting more than 6 weeks in the absence of discernible skin lesions. Pregabalin is used to treat patients with CPUO. In this study, we aimed to investigate differences in the perception threshold of itch sensation between patients with CPUO and healthy individuals and to evaluate the efficacy of pregabalin for CPUO. At baseline, week 2, and week 4 after treatment initiation, the visual analogue scale (VAS) score was measured to assess pruritus severity, and electric current perception threshold (CPT) was measured at 250 and 5 Hz using a NEUROMETER CPT/C stimulator. Twenty healthy individuals and 41 patients with CPUO were enrolled in this study. The patients with CPUO were categorised as those who responded to antihistamines (Antihistamine group), were not improved by antihistamines (Pregabalin group), and were not improved by antihistamines and pregabalin (Refractory group). The baseline CPT values were not significantly different between patients with CPUO and healthy control. Pruritus was improved in 7 of 10 patients in the Pregabalin group after treatment with pregabalin, showing decreased CPT at 5 Hz. The sensitive C-fibres presented a high threshold to detect itch sensation, and this sensitivity decreased in response to treatment with pregabalin.
Rotenone-induced inner retinal degeneration via presynaptic activation of voltage-dependent sodium and L-type calcium channels in rats
Authors: Sasaoka Masaaki , Ota Takashi , Kageyama Masaaki
Published: January 2020

Rotenone, a mitochondrial complex I inhibitor, causes retinal degeneration via unknown mechanisms. To elucidate the molecular mechanisms of its action, we further characterized a rat model of rotenone-induced retinal degeneration. Intravitreal injection of rotenone (2 nmol/eye) damaged mainly the inner retinal layers, including cell loss in the ganglion cell and inner nuclear layers, which were very similar to those induced by 10 nmol/eye N-methyl-D-aspartate (NMDA). These morphological changes were accompanied by the reduced b-wave amplitude of electroretinogram, and increased immunostaining of 2,4-dinitrophenyl, an oxidative stress marker. Rotenone also downregulated expression of neurofilament light-chain gene (Nfl) as a retinal ganglion cell (RGC) marker. This effect was prevented by simultaneous injection of rotenone with antioxidants or NMDA receptor antagonists. More importantly, voltage-dependent sodium and L-type calcium channel blockers and intracellular calcium signaling modulators remarkably suppressed rotenone-induced Nfl downregulation, whereas none of these agents modified NMDA-induced Nfl downregulation. These results suggest that rotenone-induced inner retinal degeneration stems from indirect postsynaptic NMDA stimulation that is triggered by oxidative stress-mediated presynaptic intracellular calcium signaling via activation of voltage-dependent sodium and L-type calcium channels.
Fluctuation of photon-releasing with ligand coordination in polyacrylonitrile-based electrospun nanofibers
Authors: Yu Zhimin , Shen Lifan , Li Desheng , Pun Edwin Yue Bun , Zhao Xin , Lin Hai
Published: January 2020

Multivariate terbium-complexes were incorporated into polyacrylonitrile (PAN) and electrospun into flexible multifunctional nanofibers with a uniform diameter of ~200 nm. Fluorescence comparison in multi-ligand-binding nanofibers under ultraviolet (UV) radiation verifies that the differentiated β-diketone ligands with dual functions are the primary cause of the spectral fluctuation, adequately illustrating the available methods for the quantification of intermolecular reciprocities between organic ligands and central Tb3+ions. Especially under 308 nm UVB-LED pumping, the total emission spectral power of supramolecular Tb-complexes/PAN nanofibers are identified to be 2.88 µW and the total emission photon number reaches to 7.94 × 1012 cps which are nearly six times higher than those of the binary complex ones in the visible region, respectively. By modifying the sorts of organic ligands, the luminous flux and luminous efficacy of multi-ligand Tb-complexes/PAN nanofibers are up to 1553.42 μlm and 13.72 mlm/W, respectively. Efficient photon-releasing and intense green-emission demonstrate that the polymer-capped multi-component terbium-complexes fibers have potential prospects for making designable flexible optoelectronic devices.
Author Correction: Expression analysis of cellulose synthase-like genes in durum wheat
Authors: Marcotuli Ilaria , Colasuonno Pasqualina , Blanco Antonio , Gadaleta Agata
Published: January 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Being Born Large for Gestational Age is Associated with Increased Global Placental DNA Methylation
Authors: Dwi Putra S. E. , Reichetzeder C. , Hasan A. A. , Slowinski T. , Chu C. , Krämer B. K. , Kleuser B. , Hocher B.
Published: January 2020

Being born small (SGA) or large for gestational age (LGA) is associated with adverse birth outcomes and metabolic diseases in later life of the offspring. It is known that aberrations in growth during gestation are related to altered placental function. Placental function is regulated by epigenetic mechanisms such as DNA methylation. Several studies in recent years have demonstrated associations between altered patterns of DNA methylation and adverse birth outcomes. However, larger studies that reliably investigated global DNA methylation are lacking. The aim of this study was to characterize global placental DNA methylation in relationship to size for gestational age. Global DNA methylation was assessed in 1023 placental samples by LC-MS/MS. LGA offspring displayed significantly higher global placental DNA methylation compared to appropriate for gestational age (AGA; p < 0.001). ANCOVA analyses adjusted for known factors impacting on DNA methylation demonstrated an independent association between placental global DNA methylation and LGA births (p < 0.001). Tertile stratification according to global placental DNA methylation levels revealed a significantly higher frequency of LGA births in the third tertile. Furthermore, a multiple logistic regression analysis corrected for known factors influencing birth weight highlighted an independent positive association between global placental DNA methylation and the frequency of LGA births (p = 0.001).
Quantitative monitoring of leaf area index in wheat of different plant types by integrating NDVI and Beer-Lambert law
Authors: Tan Chang-Wei , Zhang Peng-Peng , Zhou Xin-Xing , Wang Zhi-Xiang , Xu Zi-Qiang , Mao Wei , Li Wen-Xi , Huo Zhong-Yang , Guo Wen-Shan , Yun Fei
Published: January 2020

Normalized difference vegetation index (NDVI) is one of the most important vegetation indices in crop remote sensing. It features a simple, fast, and non-destructive method and has been widely used in remote monitoring of crop growing status. Beer-Lambert law is widely used in calculating crop leaf area index (LAI), however, it is time-consuming detection and low in output. Our objective was to improve the accuracy of monitoring LAI through remote sensing by integrating NDVI and Beer-Lambert law. In this study, the Beer-Lambert law was firstly modified to construct a monitoring model with NDVI as the independent variable. Secondly, experimental data of wheat from different years and various plant types (erectophile, planophile and middle types) was used to validate the modified model. The results showed that at 130 DAS (days after sowing), the differences in NDVI, leaf area index (LAI) and extinction coefficient (k) of the three plant types with significantly different leaf orientation values (LOVs) reached the maximum. The NDVI of the planophile-type wheat reached saturation earlier than that of the middle and erectophile types. The undetermined parameters of the model (LAI = −ln (a1 × NDVI + b1)/(a2 × NDVI + b2)) were related to the plant type of wheat. For the erectophile-type cultivars (LOV ≥ 60°), the parameters for the modified model were, a1 = 0.306, a2 = −0.534, b1 = −0.065, and b2 = 0.541. For the middle-type cultivars (30° < LOV < 60°), the parameters were, a1 = 0.392, a2 = −0.881, b1 = 0.028, and b2 = 0.845. And for the planophile-type cultivars (LOV ≤ 30°), those parameters were, a1 = 0.596, a2 = −1.306, b1 = 0.014, and b2 = 1.130. Verification proved that the modified model based on integrating NDVI and Beer-Lambert law was better than Beer-Lambert law model only or NDVI-LAI direct model only. It was feasible to quantitatively monitor the LAI of different plant-type wheat by integrating NDVI and Beer-Lambert law, especially for erectophile-type wheat (R2 = 0.905, RMSE = 0.36, RE = 0.10). The monitoring model proposed in this study can accurately reflect the dynamic changes of plant canopy structure parameters, and provides a novel method for determining plant LAI.
Evolutionary history of DNA methylation related genes in chordates: new insights from multiple whole genome duplications
Authors: Liu Jingwei , Hu Huihua , Panserat Stéphane , Marandel Lucie
Published: January 2020

DNA methylation is an important epigenetic mechanism involved in many biological processes, i.e. gametogenesis and embryonic development. However, increased copy numbers of DNA methylation related genes (dnmt, tet and tdg) have been found during chordate evolution due to successive whole genome duplication (WGD) events. Their evolutionary history and phylogenetic relationships remain unclear. The present study is the first to clarify the evolutionary history of DNA methylation genes in chordates. In particular, our results highlight the fixation of several dnmt3-related genes following successive WGD throughout evolution. The rainbow trout genome offered a unique opportunity to study the early evolutionary fates of duplicated genes due to a recent round of WGD at the radiation of salmonids. Differences highlighted in transcriptional patterns of these genes during gametogenesis and ontogenesis in trout indicated that they might be subjected to sub- or neo-functionalisation after WDG. The fixation of multiple dnmt3 genes in genomes after WGD could contribute to the diversification and plastic adaptation of the teleost.
Enhancing biosensing sensitivity of metal nanostructures through site-selective binding
Authors: Jo Na rae , Shin Yong-Beom
Published: January 2020

The localised surface plasmon resonance (LSPR) at the surface of metal nanostructures can induce a highly intense electromagnetic (EM) field, which is confined to the edges with big curvature or at narrow gaps between nanostructures. Therefore, the localisation of target molecules at these sites is crucial to achieve high sensitivity in LSPR-based biosensors. To this end, we fabricated a 40 nm high gold nano-truncated cone (GNTC) array using thermal nanoimprint lithography. As the EM field is most intense at the side surface and relatively weak at the top surface of GNTC, we improved the detection sensitivity by blocking the top surface with oxides to limit adsorption of antibodies and antigens to the top surface. We observed the difference in sensitivity by detecting α-fetoprotein (AFP) on the oxide-capped and uncapped GNTC arrays through sandwich immunoassay and enzymatic precipitation. The capped GNTC array exhibited higher detection sensitivity than the uncapped one. Particularly, six-fold enhancement of sensitivity was achieved in the serum sample. We used atomic force microscopy and electron microscopy to validate that the deposition of the oxides on the top surface of GNTC effectively blocked the adsorption of the biomolecules and the target molecules were preferentially adsorbed on the side surfaces.