bluuebunny/crossref_metadata_2025 huggingface dataset:
Multicolor emission based on a N, N′—Disubstituted dihydrodibenzo [a, c] phenazine crown ether macrocycle
Authors: Ma Chang-Shun , Yu Chengyuan , Zhao Cai-Xin , Zhou Shang-Wu , Gu Ruirui
Published: December 2022

Dynamic fluorophore 9,14-diphenyl-9,14-dihydrodibenzo[a,c]phenazine (DPAC) affords a new platform to produce diverse emission outputs. In this paper, a novel DPAC-containing crown ether macrocycle D-6 is synthesized and characterized. Host-guest interactions of D-6 with different ammonium guests produced a variety of fluorescence with hypsochromic shifts up to 130 nm, which are found to be affected by choice of solvent or guest and host/guest stoichiometry. Formation of supramolecular complexes were confirmed by UV-vis titration, 1H NMR and HRMS spectroscopy.
Avoiding the “Great Filter”: An assessment of climate change solutions and combinations for effective implementation
Authors: Zhang Junze , Zhang Kerry K. , Zhang Mary , Jiang Jonathan H. , Rosen Philip E. , Fahy Kristen A.
Published: December 2022

Global climate temperatures have unmistakably risen, and naturally occurring climate variability alone cannot account for this trend. Human activities are estimated to have caused about 1°C of global warming above the preindustrial baseline, and if left unchecked, it will continue to drastically damage the Earth and its inhabitants. Attempts toward alleviating the effects of global warming have often been at odds and remain divided among a multitude of strategies, reducing the overall effectiveness of these efforts. It is evident that collaborative action is required for avoiding the most severe consequences of climate change. This article evaluates the main strategies (industrial/energy, political, economic, agricultural, atmospheric, geological, coastal, and social) toward both mitigating and adapting to climate change. Also, it provides an optimal combination of seven solutions that can be implemented simultaneously, working in tandem to limit and otherwise accommodate the harmful effects of climate change. Previous legislation and deployment techniques are also discussed as guides for future endeavors.
Field observations and remote assessment identify climate change, recreation, invasive species, and livestock as top threats to critically imperiled rare plants in Nevada
Authors: McClinton Jamey D. , Kulpa Sarah M. , Grames Eliza M. , Leger Elizabeth A.
Published: December 2022

Introduction

Rare plant species comprise >36.5% of the world’s flora and disproportionately support ecosystem function and resilience. However, rare species also lead global plant extinctions, and unique ecological characteristics can make them vulnerable to anthropogenic pressure. Despite their vulnerability, many rare plants receive less monitoring than is needed to inform conservation efforts due to limited capacity for field surveys.

Methods

We used field observations and geospatial data to summarize how 128 imperiled, rare vascular plant species in Nevada are affected by various threats. We assessed correlations between threats predicted by geospatial data and threats observed on the ground and asked how historic and current threats compare.

Results

The most commonly observed threats were from recreation, invasive and non-native/alien species, and livestock farming and ranching. Threat prevalence varied by elevation (e.g., a greater variety of threats at lower elevations, greater threat from climate change observed at higher elevations) and land management. There was a 28.1% overall correlation between predicted and observed threats, which was stronger for some threats (e.g., development of housing and urban areas, livestock farming and ranching) than others. All species experienced extreme climatic differences during 1990-2020 compared to baseline conditions, with the most extreme change in southern Nevada. The average number of threats observed per occurrence increased by 0.024 each decade.

Discussion

While geospatial data did not perfectly predict observed threats, many of these occurrences have not been visited in over 30 years, and correlations may be stronger than we were able to detect here. Our approach can be used to help guide proactive monitoring, conservation, and research efforts for vulnerable species.
Development and validation of a novel risk model for predicting atrial fibrillation recurrence risk among paroxysmal atrial fibrillation patients after the first catheter ablation
Authors: Li Guangling , Wang Xiaomei , Han Jing-jing , Guo Xueya
Published: December 2022

Aims

Several models have been developed to predict the risk of atrial fibrillation (AF) recurrence after radiofrequency catheter ablation (RFCA). However, these models are of poor quality from the start. We, therefore, aimed to develop and validate a predictive model for post-operative recurrence of AF.

Materials and methods

In a study including 433 patients undergoing the first circumferential pulmonary vein isolation (CPVI) procedure, independent predictors of AF recurrence were retrospectively identified. Using the Cox regression of designated variables, a risk model was developed in a random sample of 70% of the patients (development cohort) and validated in the remaining (validation cohort) 30%. The accuracy and discriminative power of the predictive models were evaluated in both cohorts.

Results

During the established 12 months follow-up, 134 patients (31%) recurred. Six variables were identified in the model including age, coronary artery disease (CAD), heart failure (HF), hypertension, transient ischemic attack (TIA) or cerebrovascular accident (CVA), and left atrial diameter (LAD). The model showed good discriminative power in the development cohort, with an AUC of 0.77 (95% confidence interval [CI], 0.69–0.86). Furthermore, the model shows good agreement between actual and predicted probabilities in the calibration curve. The above results were confirmed in the validation cohort. Meanwhile, decision curve analysis (DCA) for this model also demonstrates the advantages of clinical application.

Conclusion

A simple risk model to predict AF recurrence after ablation was developed and validated, showing good discriminative power and calibration.
One-year results from the Assessing MICRO-vascular resistances via IMR to predict outcome in ST-elevation myocardial infarction patients with multivessel disease undergoing primary PCI (AMICRO) trial
Authors: Fineschi Massimo , Verna Edoardo , Barioli Alberto , Mezzapelle Giuseppe , Bartolini Davide , Turiano Giovanni , Guiducci Vincenzo , Manari Antonio , Lucarelli Katya , Uguccioni Lucia , Repetto Alessandra , Tarantini Giuseppe
Published: December 2022

Background

In ST-elevation myocardial infarction (STEMI) patients undergoing primary percutaneous coronary angioplasty (PPCI) the index of microcirculatory resistance (IMR) correlates to the extent of myocardial damage and left ventricular (LV) function recovery. Data on the IMR time-course and impact on clinical outcome in STEMI patients with multi-vessel disease (MVD) are scarce.

Aims

We designed a prospective, multicenter clinical trial to assess the infarct-related artery (IRA)-IMR in STEMI patients with MVD undergoing PPCI and to explore its potential in relationship with outcome and LV remodeling.

Methods

The study enrolled 242 STEMI patients with MVD. Both fractional flow reserve (FFR) and IMR of the IRA were assessed after successful PPCI. Then, FFR/IMR measurements were repeated in the IRA at a staged angiography, and FFR-guided angioplasty was performed in non-IRA lesions. The primary endpoint was the composite of cardiovascular death, re-infarction, re-hospitalization for heart failure, resuscitation or appropriate ICD shock at 1-year follow-up.

Results

A significant improvement of IRA-IMR values (from 47.9 to 34.2, p < 0.0001) was observed early after PPCI. Staged FFR-guided angioplasty was performed in 102 non-IRA lesions. We failed to find a correlation between IRA-IMR, clinical events and LV remodeling. Notwithstanding, in patients with anterior STEMI an inverse correlation between initial IMR values and LV function at follow-up was observed.

Conclusion

After successful PPCI, a significant proportion of patients with STEMI and MVD had coronary microvascular dysfunction as assessed by IMR that recovered early after reperfusion. Higher IMR values predicted lack of improvement of LV function only in anterior STEMI.

Clinical trial registration

https://clinicaltrials.gov/, identifier [NCT 02325973].
Tenuous transcriptional threshold of human sex determination. II. SRY exploits water-mediated clamp at the edge of ambiguity
Authors: Racca Joseph D. , Chatterjee Deepak , Chen Yen-Shan , Rai Ratan K. , Yang Yanwu , Georgiadis Millie M. , Haas Elisha , Weiss Michael A.
Published: December 2022

Y-encoded transcription factor SRY initiates male differentiation in therian mammals. This factor contains a high-mobility-group (HMG) box, which mediates sequence-specific DNA binding with sharp DNA bending. A companion article in this issue described sex-reversal mutations at box position 72 (residue 127 in human SRY), invariant as Tyr among mammalian orthologs. Although not contacting DNA, the aromatic ring seals the domain’s minor wing at a solvent-exposed junction with a basic tail. A seeming paradox was posed by the native-like biochemical properties of inherited Swyer variant Y72F: its near-native gene-regulatory activity is consistent with the father’s male development, but at odds with the daughter’s XY female somatic phenotype. Surprisingly, aromatic rings (Y72, F72 or W72) confer higher transcriptional activity than do basic or polar side chains generally observed at solvated DNA interfaces (Arg, Lys, His or Gln). Whereas biophysical studies (time-resolved fluorescence resonance energy transfer and heteronuclear NMR spectroscopy) uncovered only subtle perturbations, dissociation of the Y72F complex was markedly accelerated relative to wild-type. Studies of protein-DNA solvation by molecular-dynamics (MD) simulations of an homologous high-resolution crystal structure (SOX18) suggest that Y72 para-OH anchors a network of water molecules at the tail-DNA interface, perturbed in the variant in association with nonlocal conformational fluctuations. Loss of the Y72 anchor among SRY variants presumably “unclamps” its basic tail, leading to (a) rapid DNA dissociation despite native affinity and (b) attenuated transcriptional activity at the edge of sexual ambiguity. Conservation of Y72 suggests that this water-mediated clamp operates generally among SRY and metazoan SOX domains.
Circadian secretion rhythm of GLP-1 and its influencing factors
Authors: Liu Chuanfeng , Liu Yuzhao , Xin Yu , Wang Yangang
Published: December 2022

Circadian rhythm is an inherent endogenous biological rhythm in living organisms. However, with the improvement of modern living standards, many factors such as prolonged artificial lighting, sedentarism, short sleep duration, intestinal flora and high-calorie food intake have disturbed circadian rhythm regulation on various metabolic processes, including GLP-1 secretion, which plays an essential role in the development of various metabolic diseases. Herein, we focused on GLP-1 and its circadian rhythm to explore the factors affecting GLP-1 circadian rhythm and its potential mechanisms and propose some feasible suggestions to improve GLP-1 secretion.
The novel compound heterozygous rare variants may impact positively selected regions of TUBGCP6, a microcephaly associated gene
Authors: Chen Jianhai , Ying Lijuan , Zeng Li , Li Chunyu , Jia Yangying , Yang Hao , Yang Guang
Published: December 2022

Introduction

The microcephaly is a rare and severe disease probably under purifying selection due to the reduction of human brain-size. In contrast, the brain-size enlargement is most probably driven by positive selection, in light of this critical phenotypical innovation during primates and human evolution. Thus, microcephaly-related genes were extensively studied for signals of positive selection. However, whether the pathogenic variants of microcephaly-related genes could affect the regions of positive selection is still unclear.

Methods

Here, we conducted whole genome sequencing (WGS) and positive selection analysis.

Results

We identified novel compound heterozygous variants, p.Y613* and p.E1368K in TUBGCP6, related to microcephaly in a Chinese family. The genotyping and the sanger sequencing revealed the maternal and the paternal origin for the first and second variant, respectively. The p.Y613* occurred before the second and third domain of TUBGCP6 protein, while p.E1368K located within the linker region of the second and third domain. Interestingly, using multiple positive selection analyses, we revealed the potential impacts of these variants on the regions of positive selection of TUBGCP6. The truncating variant p.Y613* could lead to the deletions of two positively selected domains DUF5401 and Spc97_Spc98, while p.E1368K could impose a rare mutation burden on the linker region between these two domains.

Discussion

Our investigation expands the list of candidate pathogenic variants of TUBGCP6 that may cause microcephaly. Moreover, the study provides insights into the potential pathogenic effects of variants that truncate or distribute within the positively selected regions.
Male sex pheromone in the parasitoid wasp Nasonia longicornis: Chemical and behavioral analyses
Authors: Kurtanovic Alisa , Hofferberth John , Ruther Joachim
Published: December 2022

The use of sex pheromones for the attraction of potential mating partners is widespread in insects. Species-specificity of these chemical signals is essential, particularly in closely related species with overlapping habitats. In parasitoid wasps of the genus Nasonia, it is the males that produce sex pheromones in their rectal vesicles. The genus consists of four species: N. vitripennis (Nv), N. giraulti (Ng), N. oneida (No), and N. longicornis (Nl). The cosmopolitan species Nv is sympatric with Ng and No in eastern North America and with Nl in the west. Interspecific courtship is common in Nasonia although hybridization is prevented in most combinations by Wolbachia-mediated cytoplasmic incompatibility. The pheromone of Nv males differs from all Nasonia congeners by an additional component that is believed to ensure precopulatory isolation from the sympatric congeners. Detailed investigations on these interactions, however, exist only for the species combination Nv/Ng. Here, we report the results of chemical and behavioral investigations on the sex pheromone of Nl males. The pheromone consists of (4R,5S)-5-hydroxy-4-decanolide (RS) and 4-methylquinazoline (MQ) as a minor component which are produced only after eclosion. Pheromone titers peaked 2 days after eclosion and remained constant on day three. The pheromone is deposited by abdomen dipping which males increasingly exhibited after mating or brief contact with a female. The presence of hosts containing females about to emerge did not increase marking behavior. Site fidelity of males at their own pheromone markings is mediated by MQ. Both natural and synthetic pheromone attracted virgin but not mated females and both RS and MQ are required for female attraction. Females collected during emergence from the host responded likewise to the pheromone and 84% of them produced mixed-sex offspring showing that most of them emerge unmated. Nl females preferred pheromone extract from conspecific males to extract from Nv males, and the addition of the Nv-specific component (4R,5R)-5-hydroxy-4-decanolide (RR) to the Nl pheromone made them unresponsive. The present study demonstrates that Nl uses the male sex pheromone in a similar way as previously shown for Nv and Ng. Furthermore, it shows that Nl females use the Nv-specific pheromone component RR to avoid costly sexual interactions with Nv males in regions of sympatry.
Extent of male involvement and associated factors in antenatal care service utilization in Bench Sheko zone, Southwest Ethiopia: A community-based cross-sectional study
Authors: Mekonen Mengistu , Shifera Nigusie , Bogale Biruk , Assefa Ashenafi
Published: December 2022

Background

In many low- and middle-income countries (LMIC), men are the key decision-makers and chief providers, often determining women's access to economic resources and maternal health services. Despite the important role of men in maternal healthcare, the involvement of male partners in maternal and child health in LMIC, including Ethiopia, is low.

Objectives

This study aims to assess the extent of male involvement and associated factors in antenatal care (ANC) service utilization in the Bench Sheko zone, Southwest, Ethiopia.

Methods

A community-based, cross-sectional study was conducted in the Bench Sheko zone from February to May 2021. A multistage sampling technique was employed to select study participants. Variables with p-values <0.25 in binary logistic regression were selected as candidates for multiple logistic regression to determine independent factors associated with male involvement adjusting for sociodemographic, health service, personal, environmental, and knowledge-related factors.

Results

A total of 816 men took part in the study, with a 98.2% response rate. The prevalence of male involvement in ANC utilization was 38.2%. In multivariate analyses, residence (urban), age (25–34), education (diploma and above), income (≥2,500 Ethiopian Birr, ETB), and high knowledge of the advantages of ANC were shown to be positively associated with male involvement in ANC utilization.

Conclusion

Male involvement in ANC utilization was low. Considering the importance of male involvement in maternal healthcare, we advocate for policies and strategies that can improve knowledge of the advantages of ANC among men and can enhance their engagement in maternal care. Special attention should be given to younger partners and those partners who live in rural areas.
Bidirectional two-sample Mendelian randomization study of causality between rheumatoid arthritis and myocardial infarction
Authors: Guo Hao-Yang , Wang Wei , Peng Hui , Yuan Hui
Published: December 2022

Background

Epidemiological evidence suggests an association between rheumatoid arthritis (RA) and myocardial infarction (MI). However, causality remains uncertain. Therefore, this study aimed to explore the causal association between RA and MI.

Methods

Using publicly available genome-wide association study summary datasets, bidirectional two-sample Mendelian randomization (TSMR) was performed using inverse-variance weighted (IVW), weighted median, MR-Egger regression, simple mode, and weighted mode methods.

Results

The MR results for the causal effect of RA on MI (IVW, odds ratio [OR] = 1.041, 95% confidence interval [CI]: 1.007–1.076, P = 0.017; weighted median, OR = 1.027, 95% CI: 1.006–1.049, P = 0.012) supported a causal association between genetic susceptibility to RA and an increased risk of MI. MR results for the causal effect of MI on RA (IVW, OR = 1.012, 95% CI: 0.807–1.268, P = 0.921; weighted median, OR = 1.069, 95% CI: 0.855–1.338, P = 0.556) indicated that there was no causal association between genetic susceptibility to MI and an increased risk of RA.

Conclusion

Bidirectional TSMR analysis supports a causal association between genetic susceptibility to RA and an increased risk of MI but does not support a causal association between genetic susceptibility to MI and an increased risk of RA.
Autoimmunity in monogenic combined immune deficiencies with associated or syndromic features
Authors: Sharifinejad Niusha , Azizi Gholamreza , Chavoshzadeh Zahra , Mahdaviani Seyed Alireza , Alan Mahnaz Seifi , Tavakol Marzieh , Sadri Homa , Nabavi Mohammad , Ebrahimi Sareh Sadat , Shirkani Afshin , Vosughi Motlagh Ahmad , Safarirad Molood , Aghamahdi Fatemeh , Nazari Farzad , Delavari Samaneh , Jamee Mahnaz , Fayyaz Farimah , Samimisedeh Parham , Matani Rahman , Esmaeili Marzie , Yazdani Reza , Rezaei Nima , Abolhassani Hassan
Published: December 2022

Background

Combined immune deficiencies (CIDs) with associated or syndromic features are a highly heterogeneous subgroup of inherited immune disorders. These patients represent specific clinical complications with an increased risk of autoimmune conditions.

Methods

We analyzed data of monogenic patients with syndromic CIDs adopted from the Iranian inborn errors of immunity registry up to January 2022. A comprehensive comparison in terms of demographic, clinical, and immunological features was performed between patients with and without autoimmunity and also among four mutation groups with the most registered cases including ATM, STAT3 (AD-LOF), DNMT3B/ZBTB24, and WAS mutations.

Results

A total of 137 patients with monogenic syndromic CIDs were included. Most commonly mutated genes were the ATM [80 (58.4%)] and STAT3 (AD-LOF) [19 (13.9%)], followed by DNMT3B [11 (8%)], and WAS [11 (8%)]. More than 18% of all patients with syndromic CIDs, including most DNMT3B/ZBTB24 mutations patients, were clinically diagnosed with antibody deficiencies before genetic evaluation. Patients with ATM and WAS mutations had the latest age of onset and the lowest age of diagnosis, respectively. Autoimmune disorders were diagnosed in 24 patients at a median age of 3.5 (2.6-6.0) years, 70.6% of which were diagnosed prior to the diagnosis of immunodeficiency. Lymphoproliferation, particularly hepatosplenomegaly, was significantly higher in patients with autoimmunity (p=0.004). Syndromic CID patients with autoimmunity had significantly lower IgG levels. Hematologic autoimmunity mainly immune thrombocytopenic purpura was the most frequent autoimmunity among major groups of ATM, STAT3 (AD-LOF), DNMT3B/ZBTB24, and WAS mutations, however ATM-mutated patients present more diversified involved organs including rheumatologic, gastrointestinal and dermatologic autoimmunity.

Conclusion

About 18% of patients with monogenic syndromic CIDs developed autoimmunity, mainly in the form of hematological immune diseases. Autoimmunity could be an early-onset involvement with a potential diagnostic impact on suspicious cases of syndromic CIDs.
Sensitivity analysis of seismic attributes and oil reservoir predictions based on jointing wells and seismic data – A case study in the Taoerhe Sag, China
Authors: Li Na , Zhang Jinliang , Matsushima Jun , Song Cheng , Luan Xuwei , Dou Ming , Chen Tao , Wang Lingling
Published: December 2022

It is important to identify the location of gravelly sandstone in Taoerhe Sag, an oil target area in the Chezhen Depression. To date, most attention has focused on sedimentary characteristics; hence, information regarding a clear sensitivity analysis of seismic attributes is incomplete. To address this, we used well analysis and seismic attribute interpretation to find sensitive seismic attributes and sand bodies. Based on the well analysis and core data, a geologicald model of mudstone and sandstone was established, and the electrical characteristics of three kinds of lithology were identified. We used correlation analysis to select the optimal logging parameters and seismic attributes to identify sandstone. We used multi-attribute fusion technology and stratigraphic slices to characterize the spatial distribution characteristics of gravelly sandstone in the target area and used genetic algorithm inversion volume to evaluate the prediction results. The results indicate that the three main lithologies of the lower submember of Shahejie Formation 3 (P2S3X) in the Taoerhe Sag can be distinguished by natural gamma (GR), acoustic time difference (AC) and saturated hydrocarbon content (SH) logging curves. The seismic attribute characteristics of gravelly sandstone of P2S3X are high root mean square amplitude (RMS) values, high instantaneous bandwidth (BW) values, low 3D curvature (Curv) values, medium-high instantaneous phase (Phase) values and high instantaneous frequency (Freq) values. In this study, we found two nearshore underwater fans in P2S3X. Gravelly sandstone is located along the slope zone and the lake bottom, with a total sedimentary area of 57.9 km2. The method summarized in this paper can be applied to other similar deep-water basins.
Analysis and prediction of marine heatwaves in the Western North Pacific and Chinese coastal region
Authors: Yang Yifei , Sun Wenjin , Yang Jingsong , Lim Kam Sian Kenny T. C. , Ji Jinlin , Dong Changming
Published: December 2022

Over the past decade, marine heatwaves (MHWs) research has been conducted in almost all of the world’s oceans, and their catastrophic effects on the marine environment have gradually been recognized. Using the second version of the Optimal Interpolated Sea Surface Temperature analysis data (OISSTV2) from 1982 to 2014, this study analyzes six MHWs characteristics in the Western North Pacific and Chinese Coastal region (WNPCC, 100°E ∼ 180°E, 0° ∼ 65°N). MHWs occur in most WNPCC areas, with an average frequency, duration, days, cumulative intensity, maximum intensity, and mean intensity of 1.95 ± 0.21 times/year, 11.38 ± 1.97 days, 22.06 ± 3.84 days, 18.06 ± 7.67 °Cdays, 1.84 ± 0.50°C, and 1.49 ± 0.42 °C, respectively, in the historical period (1982 ~ 2014). Comparing the historical simulation results of 19 models of the Coupled Model Intercomparison Project Phase 6 (CMIP6) with the OISSTV2 observations, five best-performing models (GFDL-CM4, GFDL-ESM4, AWI-CM-1-1-MR, EC-Earth3-Veg, and EC-Earth3) are selected for MHWs projection (2015 ~ 2100). The MHWs characteristics projections from these five models are analyzed in detail under the Shared Socio-economic Pathway (SSP) 1-2.6, 2-4.5 and 5-8.5 scenarios. The projected MHWs characteristics under SSP5-8.5 are more considerable than those under SSP1-2.6 and 2-4.5, except for the MHWs frequency. The MHWs cumulative intensity is 96.36 ± 56.30, 175.44 ± 92.62, and 385.22 ± 168.00 °Cdays under SSP1-2.6, 2-4.5 and 5-8.5 scenarios, respectively. This suggests that different emission scenarios have a crucial impact on MHW variations. Each MHWs characteristic has an obvious increasing trend except for the annual occurrences. The increase rate of MHWs cumulative intensity for these three scenarios is 1.02 ± 0.83, 3.83 ± 1.43, and 6.70 ± 2.61 °Cdays/year, respectively. The MHWs occurrence area in summer is slightly smaller than in winter, but the MHWs average intensity is stronger in summer than in winter.
Spatial–temporal distribution of large-size light falling-net fisheries in the South China Sea
Authors: Qian Jing , Li Jiajun , Zhang Kui , Qiu Yongsong , Cai Yancong , Wu Qiaer , Tian Han , Ma Shengwei , Chen Zuozhi
Published: December 2022

With the rapid modernization of methods for monitoring fishing vessels, different ways of obtaining data on large-size light falling-net vessels have developed. In particular, the vessel monitoring system (VMS) may be used to track fishing activities in the South China Sea (SCS). In this study, we used VMS data to show the characterize and differences in the information transmitted when monitoring large-size falling-net fishing vessels, as well as their advantages and disadvantages. We investigated the spatial distribution of large-size light falling-net fisheries in the SCS using Beidou VMS data from 2017 to 2020. We also compared trajectories of fishing vessels from various provinces. According to these data, vessels operate in the Nansha Islands and the waters of the Pearl River Estuary, Beibu Gulf, Zhongsha, and Xisha Islands, and there is a clear seasonal pattern of fishing in the SCS. There are the differences of vessels in terms of distribution areas and ports of entry and exit. Additionally, we found the operation area of the large-size falling-net fishing vessels divided into four main fishing zones. The analysis compares the number of days of operation in the different fishing areas as well as seasonal variations. Our study shows that the operating area of large-size light falling-net vessels has extended southwards and that the number of days for which fishing vessels operate has increased compared with the last decade. Using the Beidou VMS to study the large-size light falling-net vessels in the SCS can provide more detailed scientific reference data for the SCS fisheries.
Integrative proteomics and metabolomics reveal the stress response of semicarbazide in the sea cucumber Apostichopus japonicus
Authors: Lu Lixin , Ren Lihua , Jiang Lisheng , Xu Xiaohui , Wang Weijun , Feng Yanwei , Li Zan , Yang Jianmin , Sun Guohua
Published: December 2022

Semicarbazide (SMC), also known as carbamoyl hydrazide, is a key intermediate for the organic synthesis of drugs, pesticides, and a panoply of other applications. It is also regarded as a landmark metabolite of nitrofurazone, a banned veterinary drug. SMC produced in different ways will eventually enter the ocean and become an emerging marine pollutant, affecting the physiological metabolism, behavioral activities, and even survival of aquatic organisms. Sea cucumbers are sediment-feeding organisms, and their risk of exposure to pollutants has attracted increasing attention. In this study, an integrated proteomic and metabolomic approach was used to investigate the responses of Apostichopus japonicus treated with SMC (3.72 g/L) for 72 h. After SMC treatment, the proteins and metabolites of A. japonicus intestine changed significantly. The results showed that 342 differentially expressed proteins were identified, of which 174 were upregulated, 168 were downregulated, and 74 differentially expressed metabolites, of which 62 were upregulated and 12 were downregulated. These differential proteins and metabolites were primarily involved in energy metabolism, lipid metabolism, signal transduction, immune regulation, autophagy, and apoptosis. On the basis of a combination of proteomic and metabolomic data, a hypothetical network of proteins, metabolites, and pathways in sea cucumbers was also described; the resulting network indicated several significant biological activities in response to SMC. This work offers a thorough analysis of the intricate mechanisms by which sea cucumbers respond to SMC stress and indicates numerous possible indicators for further research on creatures exposed to SMC. Further, our results provide scientific guidance for pollution control of Apostichopus japonicus culture to ensure healthy breeding.
Application of three-dimensional printing technology in renal diseases
Authors: Dai Shuxin , Wang Qi , Jiang Zhiwei , Liu Chang , Teng Xiangyu , Yan Songbai , Xia Dian , Tuo Zhouting , Bi Liangkuan
Published: December 2022

Three-dimensional (3D) printing technology involves the application of digital models to create 3D objects. It is used in construction and manufacturing and has gradually spread to medical applications, such as implants, drug development, medical devices, prosthetic limbs, and in vitro models. The application of 3D printing has great prospects for development in orthopedics, maxillofacial plastic surgery, cardiovascular conditions, liver disease, and other fields. With in-depth research on 3D printing technology and the continuous update of printing materials, this technology also shows broad development prospects in renal medicine. In this paper, the author mainly summarizes the basic theory of 3D printing technology, its research progress, application status, and development prospect in renal diseases.
Characterizing corn-straw-degrading actinomycetes and evaluating application efficiency in straw-returning experiments
Authors: Gong Xiujie , Yu Yang , Hao Yubo , Wang Qiuju , Ma Juntao , Jiang Yubo , Lv Guoyi , Li Liang , Qian Chunrong
Published: December 2022

Corn straw is an abundant lignocellulose resource and by-product of agricultural production. With the continuous increase in agricultural development, the output of corn straw is also increasing significantly. However, the inappropriate disposal of straw results in wasting of resources, and also causes a serious ecological crisis. Screening microorganisms with the capacity to degrade straw and understanding their mechanism of action is an efficient approach to solve such problems. For this purpose, our research group isolated three actinomycete strains with efficient lignocellulose degradation ability from soil in the cold region of China: Streptomyces sp. G1T, Streptomyces sp. G2T and Streptomyces sp. G3T. Their microbial properties and taxonomic status were assessed to improve our understanding of these strains. The three strains showed typical characteristics of the genus Streptomyces, and likely represent three different species. Genome functional annotation indicated that most of their genes were related to functions like carbohydrate transport and metabolism. In addition, a similar phenomenon also appeared in the COG and CAZyme analyses, with a large number of genes encoding carbohydrate-related hydrolases, such as cellulase, glycosidase and endoglucanase, which could effectively destroy the structure of lignocellulose in corn straw. This unambiguously demonstrated the potential of the three microorganisms to hydrolyze macromolecular polysaccharides at the molecular level. In addition, in the straw-returning test, the decomposing consortium composed of the three Streptomyces isolates (G123) effectively destroyed the recalcitrant bonds between the various components of straw, and significantly reduced the content of active components in corn straw. Furthermore, microbial diversity analysis indicated that the relative abundance of Proteobacteria, reportedly associated with soil antibiotic resistance and antibiotic degradation, was significantly improved with straw returning at both tested time points. The microbial diversity of each treatment was also dramatically changed by supplementing with G123. Taken together, G123 has important biological potential and should be further studied, which will provide new insights and strategies for appropriate treatment of corn straw.
Characterizing a subtropical hypereutrophic lake: From physicochemical variables to shotgun metagenomic data
Authors: Díaz-Torres Osiris , Lugo-Melchor Ofelia Yadira , de Anda José , Orozco-Nunnelly Danielle A. , Gradilla-Hernández Misael Sebastián , Senés-Guerrero Carolina
Published: December 2022

Lake Cajititlán is a subtropical and endorheic lake, which is heavily impacted by nutrient pollution. Agricultural runoff and poorly treated wastewater have entered this reservoir at alarming rates during past rainy seasons, causing the cultural eutrophication of this body of water and resulting in several massive fish kill events. In this study, shotgun metagenomic sequencing was used to examine the taxonomic and functional structure of microbial communities in Lake Cajititlán during the rainy season. Several water quality features and their interactions with microbial communities were also assessed to identify the major factors affecting the water quality and biota, specifically fish species. According to current water quality regulations, most of the physicochemical variables analyzed (dissolved oxygen, pH, Secchi disk, NH4+, NO3−, blue-green algae, total phosphorus, and chlorophyll-a) were outside of the permissible limits.Planktothrix agardhiiandMicrocystis aeruginosawere the most abundant phytoplankton species, and the dominant bacterial genera werePseudomonas,Streptomyces, andFlavobacterium, withPseudomonas fluorescens,Stenotrophomonas maltophilia, andAeromonas veroniirepresenting the most abundant bacterial species. All of these microorganisms have been reported to be potentially harmful to fish, and the latter three (P. fluorescens,S. maltophilia,A. veronii) also contain genes associated with pathogenicity in fish mortality (fur,luxS,aer,act,aha,exu,lip,ser). Genetic evidence from the microbial communities analyzed herein reveals that anthropogenic sources of nutrients in the lake altered genes involved in nitrogen, phosphorus, sulfur, and carbon metabolism, mainly at the beginning of the rainy season. These findings suggest that abiotic factors influence the structure of the microbial communities, along with the major biogeochemical cycles of Lake Cajititlán, resulting in temporal variations and an excess of microorganisms that can thrive in high-nutrient and low-oxygen environments. After reviewing the literature, this appears to be the first study that focuses on characterizing the water quality of a subtropical hypereutrophic lake through associations between physicochemical variables and shotgun metagenomic data. In addition, there are few studies that have coupled the metabolism of aquatic ecosystems with nutrient cycles.
Positive effect of Bifidobacterium animalis subsp. lactis VHProbi YB11 in improving gastrointestinal movement of mice having constipation
Authors: Cui Hongchang , Wang Qian , Feng Congrui , Guo Chaoqun , Zhang Jingyan , Bu Xinping , Duan Zhi
Published: December 2022

Introduction

The aim of this study was to investigate the effects of Bifidobacterium animalis subsp. lactis VHProbi® YB11 (YB11) on attenuating sucralfate-induced constipation in BALB/c mice. The strain of YB11 exhibited favorable tolerance of simulated gastrointestinal (GI) juice. Only 0.42 Log value declined when the live cells of YB11 were co-incubated with simulated GI juice. Meanwhile, this strain also displayed perfect ability to adhere the intestinal epithelium Caco-2 cells with adhesion index of 18.5. 24 of female mice were randomized into four groups.

Methods

The normal group (NOR) was fed with a normal diet, whereas the placebo group (PLA), positive group (POS), and probiotic group (PRO) were fed with sucralfate to induce constipation. After first successfully establishing the constipation model, groups NOR and PLA received the oral administration of saline solutions. Meanwhile, the POS and PRO groups were orally administered phenolphthalein and YB11 suspensions, respectively. Several indices, including fecal water content, GI transit time, short-chain fatty acids (SCFAs), intestinal neuropeptides level, and histopathology of colonic tissues, were investigated.

Results and Discussion

Compared with PLA, YB11 had a positive effect in increasing the fecal water content and intestinal peristalsis. Some positive trends, including the acetic and total acids level of fecal samples, and the colonic tissue histopathology, were also observed. Furthermore, YB11 had an ability to upregulate the levels of gut excitatory neuropeptides including motilin, gastrin, and substance P, whereas it downregulated the levels of inhibitory neuropeptides including endothelin-1, somatostatin, and vasoactive intestinal peptide. We conclude that the strain YB11 has a positive impact on improving gastrointestinal mobility and reducing the severity of constipation.
Prevalence of malaria resistance-associated mutations in Plasmodium falciparum circulating in 2017–2018, Bo, Sierra Leone
Authors: Leski Tomasz A. , Taitt Chris Rowe , Colston Sophie M. , Bangura Umaru , Holtz Andrew , Yasuda Chadwick Y. , Reynolds Nathanael D. , Lahai Joseph , Lamin Joseph M. , Baio Victoria , Ansumana Rashid , Stenger David A. , Vora Gary J.
Published: December 2022

Introduction

In spite of promising medical, sociological, and engineering strategies and interventions to reduce the burden of disease, malaria remains a source of significant morbidity and mortality, especially among children in sub-Saharan Africa. In particular, progress in the development and administration of chemotherapeutic agents is threatened by evolved resistance to most of the antimalarials currently in use, including artemisinins.

Methods

This study analyzed the prevalence of mutations associated with antimalarial resistance in Plasmodium falciparum from 95 clinical samples collected from individuals with clinically confirmed malaria at a hospital in Bo, Sierra Leone between May 2017 and December 2018. The combination of polymerase chain reaction amplification and subsequent high throughput DNA sequencing was used to determine the presence of resistance-associated mutations in five P. falciparum genes – pfcrt, pfmdr1, pfdhfr, pfdhps and pfkelch13. The geographic origin of parasites was assigned using mitochondrial sequences.

Results

Relevant mutations were detected in the pfcrt (22%), pfmdr1 (>58%), pfdhfr (100%) and pfdhps (>80%) genes while no resistance-associated mutations were found in the pfkelch13 gene. The mitochondrial barcodes were consistent with a West African parasite origin with one exception indicating an isolate imported from East Africa.

Discussion

Detection of the pfmdr1 NFSND haplotype in 50% of the samples indicated the increasing prevalence of strains with elevated tolerance to artemeter + lumefantrine (AL) threatening the combination currently used to treat uncomplicated malaria in Sierra Leone. The frequency of mutations linked to resistance to antifolates suggests widespread resistance to the drug combination used for intermittent preventive treatment during pregnancy.
Influence of dietary n-3 long-chain fatty acids on microbial diversity and composition of sows’ feces, colostrum, milk, and suckling piglets’ feces
Authors: Llauradó-Calero Eudald , Climent Eric , Chenoll Empar , Ballester Maria , Badiola Ignacio , Lizardo Rosil , Torrallardona David , Esteve-Garcia Enric , Tous Núria
Published: December 2022

Introduction

Very little is known about the impact of n-3 long-chain fatty acids (n-3 LCFAs) on the microbiota of sows and their piglets. The aim of this study was to evaluate the effect of n-3 LCFA in sow diets on the microbiota composition of sows’ feces, colostrum, and milk as well as that of piglets’ feces.

Methods

Twenty-two sows were randomly assigned to either a control or an n-3 LCFA diet from service to weaning. Sows’ and piglets’ performance was monitored. The gestating and lactating sows’ microbiomes in feces, colostrum, and milk were characterized by 16s ribosomal RNA gene sequencing. The fecal microbiome from the two lowest (>800 g) and the two highest birth weight piglets per litter was also characterized, and the LPS levels in plasma were analyzed at weaning.

Results and Discussion

n-3 LCFA increased microbiota alpha diversity in suckling piglets’ and gestating sows’ feces. However, no effects were observed in colostrum, milk, or lactating sows’ feces. Dietary n-3 LCFA modified the microbiota composition of gestating sows’ feces, milk, and suckling piglets’ feces, without affecting lactating sows’ feces or colostrum. In gestating sows’ feces and milk, the decrease in genus Succinivibrio and the increase of Proteobacteria phylum, due to the increased genera Brenneria and Escherichia, respectively, stand out. In the feces of suckling piglets, the higher abundance of the beneficial genus Akkermansia and Bacteroides, and different species of Lactobacillus are highlighted. In addition, positive correlations for families and genera were found between lactating sows’ feces and milk, milk and suckling piglets’ feces, and lactating sows’ feces and suckling piglets’ feces. To conclude, dietary n-3 LCFA had a positive impact on the microbiome of suckling piglet’s feces by increasing microbial diversity and some beneficial bacteria populations, had a few minor modifications on the microbiome of milk and gestating sows’ feces and did not change the microbiome in lactating sows’ feces or colostrum. Therefore, this study shows the effect of dietary n-3 LCFA on the microbiota of sows, colostrum, milk, and suckling piglets during the lactation period providing crucial information on the microbiota status at the early stages of life, which have an impact on the post-weaning.
Application of the robot-assisted implantation in deep brain stimulation
Authors: Ma Fang-Zhou , Liu De-Feng , Yang An-Chao , Zhang Kai , Meng Fan-Gang , Zhang Jian-Guo , Liu Huan-Guang
Published: December 2022

Introduction

This work aims to assess the accuracy of robotic assistance guided by a videometric tracker in deep brain stimulation (DBS).

Methods

We retrospectively reviewed a total of 30 DBS electrode implantations, assisted by the Remebot robotic system, with a novel frameless videometric registration workflow. Then we selected 30 PD patients who used stereotactic frame surgery to implant electrodes during the same period. For each electrode, accuracy was assessed using radial and axial error.

Results

The average radial error of the robot-assisted electrode implantation was 1.28 ± 0.36 mm, and the average axial error was 1.20 ± 0.40 mm. No deaths or associated hemorrhages, infections or poor incision healing occurred.

Conclusion

Robot-assisted implantation guided by a videometric tracker is accurate and safe.
EEG as an indispensable tool during and after the COVID-19 pandemic: A review of tribulations and successes
Authors: Freund Brin E. , Feyissa Anteneh M.
Published: December 2022

During the coronavirus disease 2019 (COVID-19) pandemic, elective and non-emergent tests and procedures were delayed or suspended in lieu of diverting resources to more emergent treatment of critically ill patients and to avoid the spread and contraction of COVID-19. Further, the workforce was stretched thin, and healthcare facilities saw high turnover rates for full-time and contract employees, which strained the system and reduced the ability to provide clinical services. One of the casualties of these changes was electroencephalography (EEG) procedures, which have been performed less frequently throughout the world since the pandemic. Whether considered routine or emergent, the deferral of EEG studies can cause downstream effects, including a delay in diagnosis and initiation of treatment for epilepsy and non-epileptic seizures resulting in a higher risk of morbidity and mortality. Despite these limitations, the importance and utility of EEG and EEG technologists have been reinforced with the development of COVID-related neurological complications, including encephalopathy and seizures, which require EEG for diagnosis and treatment. Since the pandemic, reliance on remote telemonitoring has further highlighted the value and ease of using EEG. There has also been a heightened interest in rapid EEG devices that non-technologist professionals can attach quickly, allowing minimum patient contact to avoid exposure to COVID-19 and taking advantage of remote EEG monitoring. This review discusses the acute and potential long-term effects of the COVID-19 pandemic on the use and performance of EEG.
Multicenter, prospective feasibility study of Nano-Pulse Stimulation™ technology for the treatment of both nodular and superficial low-risk basal cell carcinoma
Authors: Ross Amy S. , Schlesinger Todd , Harmon Christopher B. , Moy Ronald L. , Rohrer Thomas E. , Mehregan Darius R. , Nuccitelli Richard , Johnston Lauren Jauregui , Knape William A.
Published: December 2022

Background

Nano-Pulse Stimulation™ (NPS™) therapy is a new, non-thermal bioelectric modality that applies ultrashort pulses of electric energy to trigger regulated cell death (RCD) in treated tissues. Instead of initiating necrosis by heating or freezing, NPS therapy permeabilizes intracellular organelles to activate the cell’s own self-destruct pathway of programmed or regulated cell death. Unlike cryotherapeutic procedures that can both damage structural tissues and diffuse into the periphery beyond the margins of the lesion, NPS therapy only affects cells within the treated zone leaving surrounding tissue and acellular components unaffected.

Methods

In this study we treated 37 basal cell carcinoma lesions on 30 subjects (NCT04918381). The treated lesions were photographed on 3-, 7-, 14-, 30- and 60-days after treatment. All subjects then underwent surgical excision for histological examination of the treated tissue.

Results

92% of the BCC lesions (34 of 37) showed complete histological clearance of BCC. Histologic analysis of the 3 cases where residual BCC was noted indicated that full energy coverage was not achieved, which could be remedied with an improved treatment guide to standardize and optimize the CellFX® procedure based on NPS technology.

Conclusion

The CellFX procedure was shown to be safe and effective for the treatment of low-risk nodular and superficial BCC lesions.