bluuebunny/crossref_metadata_2025 huggingface dataset:
Toxicity and Availability of Manganese in the Growth of Tropical Grasses in Contaminated Soil
Authors: Silva Enilson de Barros , Souza Willian Cleisson Lopes de , Nardis Barbara Olinda , Alleoni Luís Reynaldo Ferracciú , Grazziotti Paulo Henrique , Santos Lauana Lopes dos , Bezerra Iracema Raquel Santos , Alves Flávio Antônio Fernandes , Silva Wesley Costa , Uane Bento Gil , Nascimento Sandra Antunes do
Published: June 2024

Phytoremediation is a technique with high viability due to its low cost and minimal environmental impact. However, there are a few results regarding the use of tropical grasses in the phytoremediation of manganese (Mn), and there is no clear definition of which extractor is more suitable for assessing Mn availability in soils. The objective of this study was to evaluate the growth potential and phytoremediation of five tropical grasses in Mn‐contaminated soil, as well as to assess the efficiency of four extractors in Mn extraction. The grass cultivars evaluated included Aruana and Tanzânia of Megathyrsus maximus, Xaraés and Marandu of Urochloa brizantha, and Basilisk of Urochloa decumbens grown in Typic Hapludox (Oxisol) amended with 0, 50, 150, and 450 mg of Mn per kg−1 of soil. The experiment was conducted in a greenhouse, using a completely randomized experimental design in a 5 × 4 factorial scheme, with five replications. The experimental period lasted for 90 days. The dry matter of the shoot and root of the grasses was evaluated. Mn concentration in dry matter and its availability in soil were extracted by Mehlich‐1 and DTPA pH 7.3, while the semitotal concentration was determined by USEPA 3051 and total Mn by USEPA 3052 method. Tropical grasses showed reduced growth with increasing Mn rates in the soil, with the following sequence in the reduction in growth: Aruana > Basilisk > Marandu > Xaraés and lastly the Tanzânia. The Aruana cultivar showed greater potential for phytoextraction due to its higher ability to accumulate Mn in its biomass, followed by the Basilisk cultivar. The four different evaluated extractors were efficient in measuring Mn concentrations in the soil, with higher toxicity critical levels for the USEPA 3051 method due to the semitotal extraction nature and USEPA 3052 due to total Mn extraction. The growth of tropical grasses is reduced with increasing Mn rates applied to the soil. The Aruana of M. maximus grass has greater potential for phytoextraction of Mn‐contaminated soils, followed by Basilisk of Urochloa decumbens, Xaraés and Marandu of U. brizantha, and Tanzânia of M. maximus. The Mehlich‐1, DTPA pH 7.3, USEPA 3051, and 3052 extractors were efficient in extracting and evaluating Mn availability in soil for tropical grasses. The USEPA 3051 and 3052 methods presented higher toxicity critical levels.
Food Security Determinants and Coping Strategies in Central Highlands of Ethiopia
Authors: Alemayehu Arragaw , Tesfaye Argaw
Published: June 2024

Food insecurity has emerged as a key problem and development challenge in Ethiopia even after the country has shown some economic recovery. The main objective of this study was to analyze food security determinants and coping strategies in central highlands of Ethiopia. The data were collected from 123 households from three Kebeles, nine key informant interviews, and three focus group discussions. The methods of analysis included household food balance model (HFBM), Foster–Greer–Thorbecke (FGT), household food consumption score (HFCS), and binary logistic regression model (BLR). The results indicated that 62% of the households were food insecure. The mean calorie intake of the households was 2051.02 kcal/person/day. A statistically significant difference across the agroecological zones was observed in terms of food insecurity at the p = 0.05 level. Empirically, landholding size and soil fertility were the significant determinants of food security status at p = 0.01 level. Changes in consumption patterns and the sale of livestock are the dominant coping strategies reported by 88% and 84% of households, respectively. This study recommends changes in government policies and strategies to sustainably mitigate food security challenges by focusing on the district’s potential.
Obesity Induces Temporally Regulated Alterations in the Extracellular Matrix That Drive Breast Tumor Invasion and Metastasis
Authors: Conner Sydney J. , Borges Hannah B. , Guarin Justinne R. , Gerton Thomas J. , Yui Anna , Salhany Kenneth J. , Mensah Diamond N. , Hamilton Grace A. , Le Giang H. , Lew Katherine C. , Zhang Crystal , Oudin Madeleine J.
Published: June 2024

Obesity is associated with increased incidence and metastasis of triple-negative breast cancer, an aggressive breast cancer subtype. The extracellular matrix (ECM) is a major component of the tumor microenvironment that drives metastasis. To characterize the temporal effects of age and high-fat diet (HFD)–driven weight gain on the ECM, we injected allograft tumor cells at 4-week intervals into mammary fat pads of mice fed a control or HFD, assessing tumor growth and metastasis and evaluating the ECM composition of the mammary fat pads, lungs, and livers. Tumor growth was increased in obese mice after 12 weeks on HFD. Liver metastasis increased in obese mice only at 4 weeks, and elevated body weight correlated with increased metastasis to the lungs but not the liver. Whole decellularized ECM coupled with proteomics indicated that early stages of obesity were sufficient to induce changes in the ECM composition. Obesity led to an increased abundance of the proinvasive ECM proteins collagen IV and collagen VI in the mammary glands and enhanced the invasive capacity of cancer cells. Cells of stromal vascular fraction and adipose stem and progenitor cells were primarily responsible for secreting collagen IV and collagen VI, not adipocytes. Longer exposure to HFD increased the invasive potential of ECM isolated from the lungs and liver, with significant changes in ECM composition found in the liver with short-term HFD exposure. Together, these data suggest that changes in the breast, lungs, and liver ECM underlie some of the effects of obesity on triple-negative breast cancer incidence and metastasis.

Significance: Organ-specific extracellular matrix changes in the primary tumor and metastatic microenvironment are mechanisms by which obesity contributes to breast cancer progression.
Chronic Health Conditions, Disability, and Physical and Cognitive Limitations among LGBTQ+ Cancer Survivors
Authors: Waters Austin R. , Jin Mu , Jones Shaun R. , Datta Geetanjali D. , Butler Eboneé N. , Kent Erin E. , Nichols Hazel B. , Tan Kelly
Published: June 2024

Background:

Cancer survivors are at high risk for chronic health conditions and physical and cognitive limitations. However, few studies have explored these outcomes among Lesbian, Gay, Bisexual, Transgender, Queer, Plus (LGBTQ+) survivors.

Methods:

We used pooled, weighted Behavioral Risk Factor Surveillance System data from 23 states that completed two specific modules at least once from 2020 to 2022. We calculated age-adjusted prevalence for heart disease, asthma, chronic obstructive pulmonary disease, depressive disorders, myocardial infarction, kidney disease, stroke, diabetes, hearing disability, vision disability, cognitive limitations, and difficulty walking, dressing, and running errands in LGBTQ+, lesbian, gay, or bisexual, transgender or gender nonconforming (TGNC), and non-LGBTQ+ cancer survivors. Four multivariable logistic regression models controlling for different factors were run for each outcome.

Results:

Of 40,990 cancer survivors, 1,715 were LGBTQ+. LGBTQ+ survivors had significantly higher age-adjusted prevalence of all outcomes. The prevalence of all outcomes was the highest among TGNC survivors, except for depressive disorders and cognitive limitations. LGBTQ+ survivors had higher odds of reporting asthma [adjusted OR (aOR): 1.5; 95% confidence interval (CI), 1.2–1.9], depressive disorders (aOR: 1.9; 95% CI, 1.6–2.4), kidney disease (aOR: 1.5; 95% CI, 1.1–2.1), stroke (aOR: 1.7; 95% CI, 1.3–2.3), diabetes (aOR: 1.3; 95% CI, 1.0–1.6), vision disability (aOR: 1.6; 95% CI, 1.2–2.2), cognitive limitations (aOR: 2.3; 95% CI, 1.8–2.9), difficulty walking (aOR: 1.7; 95% CI, 1.3–2.0), dressing (aOR: 2.0; 95% CI, 1.5–2.7), and running errands (aOR: 1.6; 95% CI, 1.3–2.1). In TGNC models, TGNC cancer survivors had increased odds of most outcomes in comparison to cisgender survivors.

Conclusions:

LGBTQ+ cancer survivors have an elevated burden of all chronic health conditions, disabilities, and limitations assessed. TGNC cancer survivors experience even higher burden of the same outcomes.

Impact:

Findings highlight substantial disparities regarding the health of LGBTQ+ cancer survivors.

See related In the Spotlight, p. 1395
Use of Nonsteroidal Anti-Inflammatory Drugs and Pancreatic Cancer Risk in the Women’s Health Initiative
Authors: Brasky Theodore M. , Jager Leah R. , Newton Alison M. , Li Xilin , Loomans-Kropp Holli A. , Hays John L. , Margolis Karen L. , Luo Juhua
Published: June 2024

Background:

Pancreatic cancer is among the most fatal human cancers and the fourth leading cause of cancer death in the United States. Evidence suggests that chronic inflammation may play a role in pancreatic carcinogenesis and its inhibition through nonsteroidal anti-inflammatory drugs (NSAID) may reduce pancreatic cancer incidence.

Methods:

We examined associations of total and individual NSAIDs with pancreatic cancer risk among postmenopausal women participating in the Women’s Health Initiative observational study and clinical trial cohorts. Among 117,452 women, aged 55 to 79 years, 727 incident pancreatic cancer cases were reported over 18 years of follow-up. Cox regression was used to estimate hazard ratio (HR) and 95% confidence interval (CI) for associations between NSAIDs and pancreatic cancer risk.

Results:

Relative to non-use, consistent use of any NSAID was inversely associated with pancreatic cancer risk (HR 0.71, 95% CI, 0.59–0.87), primarily driven by strong associations for aspirin use (HR 0.67, 95% CI, 0.52–0.86). Use of total or individual non-aspirin NSAIDs was not associated with pancreatic cancer. Upon stratified analysis, we observed stronger associations for NSAIDs among participants with prevalent diabetes (HR 0.28, 95% CI, 0.10–0.75) relative to those without (HR 0.75, 95% CI, 0.61–0.92; P-interaction = 0.03).

Conclusions:

Additional large prospective studies with careful measurement of NSAID type, dose, and frequency are needed to further investigate the possibility of added benefit among individuals diagnosed with diabetes.

Impact:

This study adds to existing evidence from prospective studies and clinical trials suggesting that use of aspirin may provide moderate benefit for pancreatic cancer prevention.
Supplementary Tables S1-S12 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Supp Table ST1 - ST12
Supplementary Figure 4 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Circulating ORF1p levels in patients with IPMN
Supplementary Figure 27 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Kaplan-Meier Survival Analysis by ORF1p.
Supplementary Figure 26 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Calibration curve for the large-volume (500 µL) MOSAIC assay.
Supplementary Figure 15 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Validation of monoclonal antibodies by western blotting.
Supplementary Figure 13 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Representative SPR sensorgrams of ORF1 monoclonal antibodies and “2nd Gen.” nanobody multimers.
Supplementary Figure 10 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Representative SPR sensorgrams of ORF1 nanobodies.
Supplementary Data 2 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

Contains all Tables and Figures in one file
Supplementary Data 1 from Ultrasensitive Detection of Circulating LINE-1 ORF1p as a Specific Multicancer Biomarker
Authors: Taylor Martin S. , Wu Connie , Fridy Peter C. , Zhang Stephanie J. , Senussi Yasmeen , Wolters Justina C. , Cajuso Tatiana , Cheng Wen-Chih , Heaps John D. , Miller Bryant D. , Mori Kei , Cohen Limor , Jiang Hua , Molloy Kelly R. , Chait Brian T. , Goggins Michael G. , Bhan Irun , Franses Joseph W. , Yang Xiaoyu , Taplin Mary-Ellen , Wang Xinan , Christiani David C. , Johnson Bruce E. , Meyerson Matthew , Uppaluri Ravindra , Egloff Ann Marie , Denault Elyssa N. , Spring Laura M. , Wang Tian-Li , Shih Ie-Ming , Fairman Jennifer E. , Jung Euihye , Arora Kshitij S. , Yilmaz Osman H. , Cohen Sonia , Sharova Tatyana , Chi Gary , Norden Bryanna L. , Song Yuhui , Nieman Linda T. , Pappas Leontios , Parikh Aparna R. , Strickland Matthew R. , Corcoran Ryan B. , Mustelin Tomas , Eng George , Yilmaz Ömer H. , Matulonis Ursula A. , Chan Andrew T. , Skates Steven J. , Rueda Bo R. , Drapkin Ronny , Klempner Samuel J. , Deshpande Vikram , Ting David T. , Rout Michael P. , LaCava John , Walt David R. , Burns Kathleen H.
Published: June 2024

De-identified patient information for survival analysis.
Endoplasmic Reticulum Stress Potentiates the Immunosuppressive Microenvironment in Hepatocellular Carcinoma by Promoting the Release of SNHG6-Enriched Small Extracellular Vesicles
Authors: Liu Chengdong , Zhou Xiaohan , Zeng Hanyi , Yu Jiaping , Li Wenwen , Zhang Wanli , Liao Yanxia , Wang Haijian , Liu Li
Published: June 2024

Endoplasmic reticulum (ER) stress leads to hepatocellular carcinoma (HCC) progression. Small extracellular vesicles (sEV) play a crucial role in modulating the tumor microenvironment (TME) by influencing cellular communication and immune responses. However, it is unclear whether ER stress modulates the TME through sEVs. In the current study, we investigated the effects and underlying mechanisms of ER stress on the HCC TME. In vivo and in vitro experiments showed that overactivated ER stress was a salient attribute of the immunosuppressive HCC TME. This was caused by the ATF4-promoted release of small nucleolar RNA host gene 6 (SNHG6)-carrying sEVs, which attenuated T cell-mediated immune responses. Overall, SNHG6 modulated the immunosuppressive TME and aggravated ER stress. Meanwhile, targeting SNHG6 facilitated M1-like macrophage and CD8+ T-cell infiltration and decreased the proportion of M2-like macrophages. In addition, SNHG6 knockdown enhanced anti-PD1 immunotherapeutic efficacy. Moreover, in HCC patients, overexpression of SNHG6 was associated with a lack of response to anti-PD1 therapy and poor prognosis, whereas low SNHG6 expression was associated with improved therapeutic efficacy and prognoses. These data indicate that a correlation exists among ER stress, sEVs, immunosuppressive HCC TME, and immunotherapeutic efficacy. Hence, SNHG6-targeted therapy may represent an effective strategy for patients with HCC.
FIGURE 4 from The TERT Promoter is Polycomb-Repressed in Neuroblastoma Cells with Long Telomeres
Authors: Graham Mindy K. , Xu Beisi , Davis Christine , Meeker Alan K. , Heaphy Christopher M. , Yegnasubramanian Srinivasan , Dyer Michael A. , Zeineldin Maged
Published: June 2024

TERT is a bona fide MYCN target. A, Alignments of DNA methylation from WGBS and MYCN-ChIP-seq in three MYCN-amplified neuroblastoma O-PDX models and cell line (SKNBE2) showing that MYCN binding overlaps with the hypomethylated region in the TERT promoter. The core promoter is shaded in red and THOR is shaded in yellow. B, Western blot analysis for MYCN and GAPDH (loading control) in different indicated doxycycline-inducible engineered cell lines to ectopically express MYCN in non–MYCN-amplified cells (left) or to express three different MYCN-shRNAs (indicated as A, B, C) in MYCN-amplified neuroblastoma cells (right). Relative MYCN band intensities in cells expressing MYCN-shRNA are indicated on top of the blots. C, A schematic representation of Luciferase reporter construct. The long construct contains the luciferase gene, and the TERT promoter core and THOR sequences, while the short construct does not contain the THOR promoter sequence. D, Fold change of Luciferase luminescence for the short construct (top) and the long construct (bottom) compared with control vehicle-treated controls. E, Fold change of TERT expression in the same cells measured by qRT-PCR. *, P < 0.05, error bars represent SD.
FIGURE 2 from The TERT Promoter is Polycomb-Repressed in Neuroblastoma Cells with Long Telomeres
Authors: Graham Mindy K. , Xu Beisi , Davis Christine , Meeker Alan K. , Heaphy Christopher M. , Yegnasubramanian Srinivasan , Dyer Michael A. , Zeineldin Maged
Published: June 2024

Molecular characterization of telomere maintenance in neuroblastoma cell lines. Telomere maintenance mechanism was assessed in eight neuroblastoma cell lines used in this study: MYCN-amplified (NB5, SKNBE2, IMR32), moderate level of MYCN without amplification (NBL-S), ATRX-mutant (SKNMM, CHLA90), low-DAXX (SKNFI) and ever-shorter telomere (LAN6) neuroblastoma cells. A, Representative images of Telomere FISH (green) showing brighter signal in cell lines with long telomeres. B, Telomeric repeats in neuroblastoma cell lines relative to those in the telomerase-positive 293T cells using qPCR. Telomerase-negative, ALT-positive U2OS cells were used as a positive control for long telomeres. C, Representative blot of C-circle assay. U2OS cells were used as a positive control and PC3 cells were used as a negative control. The amount of DNA in nanograms (ng) is indicated on the blot. All reactions were done in the presence or absence of the Phi 29 (Phi) DNA polymerase. qRT-PCR was performed in neuroblastoma cell lines to measure TERT (D) and TERC (E) expression relative to the telomerase-positive 293T cell line. Gene expression was normalized to HPRT expression. The U2OS cell line was used as an ALT-positive control and does not express TERC.
Tecnologia e escravocracia no Brasil durante o século XIX: uma reinterpretação
Authors: Pang Eul-Soo
Published: June 2024

This work attempts to revise the existing historiographical assumption that slavery and capitalism were not compatible and that for this reason the slavocracy lacked a modernizing mentality. It isargued that the Brazilian sugar plantation system was a variant of the capitalist agriculture of the larger Atlantic world-economy. Furthermore, it is argued that the Brazilian slavocracy embodied certain characteristics which have traditionally been ignored by historians but should now be viewed as integral features of the Brazilian capitalist agriculture: a desire to invest in new projects, an ability to accumulate capital, and a willingness to risk capital in order to maximize economic gains. Admittedly, not all planters developed such a mentality. Between 1790 and 1840 Brazil went through a host of crises, especially in its export agriculture, which provided both incentives and the necessity for selective agroindustrialization in the succeeding decades. Two cases of slavocratic clans, one in Bahia and another in Rio province, were chosen here to observe the regional variations as well as to generalize the attitudes of the hegemonic elite at large. The Bahian and Fluminense cases were also contrasted, when and where schematically desirable, with the experiences in other provinces such as Alagoas, Maranhão, and São Paulo. It is hypothesized that capitalist agriculture based on a coercive labor regime as in the case of Brazil did not necessarily impede a modernizing process, but rather placed limits on its growth and diversification.
Dinámicas agroindustriales en el Gran Chaco: una aproximación al espacio transfronterizo Argentina-Paraguay
Authors: Salizzi Esteban
Published: June 2024

Este trabalho contribui para o estudo dos vínculos mantidos pela expansão territorial do modelo produtivo do agronegócio na América do Sul com as continuidades e descontinuidades que organizam os bordes dos territórios nacionais. Para tanto, aborda as singularidades apresentadas pelo avanço da fronteira agrária moderna sobre a área transfronteiriça que se estrutura no Gran Chaco, concentrando a atenção no segmento definido pelo limite internacional entre Argentina e Paraguai localizado dentro de sua área de influência. Em termos temporais, o período analisado se estende entre a década de 2000 e a atualidade, em estreita relação com a disseminação dos cultivos geneticamente modificados no Cone Sul. O espaço transfronteiriço de referência, depois de ter constituído um eixo de conflito entre ambos países, representa hoje um recurso que procura ser capitalizado tanto pelas corporações agroindustriais como pelos respectivos governos. Desta forma, o Gran Chaco configura um espaço transfronteiriço, geralmente considerado marginal, que se torna global e é cada vez mais utilizado para garantir a acumulação e circulação de capital.
Freedom is a place. Ruth Wilson Gilmore e a Geografia Abolicionista
Authors: Gilmore Ruth Wilson , Antipon Livia Cangiano , Alves Cristiano Nunes , Novo Maria Fernanda
Published: June 2024

Ruth Wilson Gilmore, geógrafa estadunidense, é diretora do Center for Place, Culture and Politics (CPCP), e professora nos departamentos “Earth and Environmental Sciences”, “Africana Studies” and “American Studies” da City University of New York (CUNY – Graduate Center). Reconhecida como uma das intelectuais mais proeminentes da atualidade, dedica seu pensamento e militância ao entendimento e recusa do capitalismo racial, propondo caminhos cotidianos rumo a futuros libertários. Por meio de um rigoroso trabalho comprometido com a Tradição Radical Negra ela estrutura sua pesquisa-militante, e ampara-se em leituras de mundo que possibilitam a formação e transformação da consciência.
The Discovery of Extracellular Vesicles and Their Emergence as a Next-Generation Therapy
Authors: Rai Alin , Claridge Bethany , Lozano Jonathan , Greening David W.
Published: June 2024

From their humble discovery as cellular debris to cementing their natural capacity to transfer functional molecules between cells, the long-winded journey of extracellular vesicles (EVs) now stands at the precipice as a next-generation cell-free therapeutic tool to revolutionize modern-day medicine. This perspective provides a snapshot of the discovery of EVs to their emergence as a vibrant field of biology and the renaissance they usher in the field of biomedical sciences as therapeutic agents for cardiovascular pathologies. Rapid development of bioengineered EVs is providing innovative opportunities to overcome biological challenges of natural EVs such as potency, cargo loading and enhanced secretion, targeting and circulation half-life, localized and sustained delivery strategies, approaches to enhance systemic circulation, uptake and lysosomal escape, and logistical hurdles encompassing scalability, cost, and time. A multidisciplinary collaboration beyond the field of biology now extends to chemistry, physics, biomaterials, and nanotechnology, allowing rapid development of designer therapeutic EVs that are now entering late-stage human clinical trials.
Emerging Roles and Therapeutic Applications of Arachidonic Acid Pathways in Cardiometabolic Diseases
Authors: Hu Yufeng , Li Wei , Cheng Xu , Yang Hailong , She Zhi-Gang , Cai Jingjing , Li Hongliang , Zhang Xiao-Jing
Published: June 2024

Cardiometabolic disease has become a major health burden worldwide, with sharply increasing prevalence but highly limited therapeutic interventions. Emerging evidence has revealed that arachidonic acid derivatives and pathway factors link metabolic disorders to cardiovascular risks and intimately participate in the progression and severity of cardiometabolic diseases. In this review, we systemically summarized and updated the biological functions of arachidonic acid pathways in cardiometabolic diseases, mainly focusing on heart failure, hypertension, atherosclerosis, nonalcoholic fatty liver disease, obesity, and diabetes. We further discussed the cellular and molecular mechanisms of arachidonic acid pathway–mediated regulation of cardiometabolic diseases and highlighted the emerging clinical advances to improve these pathological conditions by targeting arachidonic acid metabolites and pathway factors.
A new species of Psychomyia (Trichoptera, Psychomyiidae) from China
Authors: TAO ZHULIN , QIU SHUANG
Published: June 2024

The male and female of a new species of the genus Psychomyia Latreille 1829, Psychomyia tahmanica sp. nov., is described based on morphological characters and molecular data (COI). Images of the adults and genitalia are provided.
3. Aesthetic Experiences of Contemporary Music from the Perspectives of a Composer, a Performer, and a Musicologist
Authors: Nikolić Sanela , Miladinović Prica Ivana
Published: June 2024

This chapter addresses the music-related knowledge and aesthetic experience of contemporary classical music from the perspectives of three music experts involved in a specific piece of music―performer, composer, and musicologist. Participants reflected on their emotional and cognitive processes and aesthetic responses to the music they are intimately familiar with and their narratives were analysed using Interpretative phenomenological analysis. The findings are congruent with previous research, where the aesthetic experiences induced by contemporary music were related to the cognitive mastering of properties of the musical structure. Furthermore, the analysis showed two types of expertise contributing to the aesthetic experience of contemporary music: theoretical knowledge―learning and acquisition of knowledge about music structure and style; and experiential knowledge―which comes from a person’s mastery of skills in appreciating music as a result of long-term, in-depth involvement with music.
14. The Personality of Music Students with Diverse Vocal and Instrumental Skills
Authors: Butković Ana
Published: June 2024

This chapter presents the results of a study that examined personality differences in a sample of music students (N = 246) with diverse vocal and instrumental skills. Personality data, measured with the Big Five Inventory (BFI), from voice, keyboard, string, and wind instrument students of the Academy of Music in Zagreb were analysed. The results indicate personality differences between different job specialisations among musicians, but they also suggest that the differences are limited to one personality trait, Openness. Individual differences in the other four personality traits were not found across music student groups.
17. Conclusion: Progressing the State of the Art of Music Psychology
Authors: Timmers Renee , Bogunović Blanka , Nikolić Sanela
Published: June 2024

Research from Western Europe tends to be better known and more frequently published than research from the Western Balkans. Various factors contribute to such imbalances of publication and recognition, including socio-economic, political, linguistic, and psychological, in the case of implicit biases. This book demonstrates the rich history of research in the psychology of music in the Western Balkans and aims to address power imbalances by explicitly promoting research from this region. In this concluding chapter, we argue for the opportunities offered by collaborative, interdisciplinary research to enable a more inclusive discipline of the psychology of music, with greater equality of contributions and embracing the diversity of knowledge and understanding. We reflect on the overlaps and differences between the research captured in this book, which originated in Western Europe and the Western Balkans. Whilst similarities and overlaps are most prevalent, subtle differences are also apparent in specific specialisms in knowledge development. Furthermore, contexts such as variations in systems and practices of music education and variations in musical preferences demonstrate the need for research to be situated rather than being assumed to be context-independent. This offers a tension for researchers to add to generalisable knowledge or develop context-dependent insights. Such tensions need to be first of all acknowledged in mainstream music psychology, highlighting the limitations of published research, and opening a larger platform for research from a diversity of regions, and cultural and research practices.
Prevalence of the generalized anxiety disorder in Tunisia: A study of 707 primary care outpatients
Authors: Ben Thabet Jihene , Turki Mariem , Mezghani Molka , Guermazi Asma , Mâalej Mohamed , Charfi Nada , Maalej Manel
Published: June 2024

Objective

This study examined the prevalence and correlates of generalized anxiety disorder (GAD) among primary care outpatients.

Method

A multicenter, cross-sectional study was conducted that included 707 primary care outpatients being seen at 20 primary healthcare centers in Tunis and Sfax, Tunisia. The Mini International Neuropsychiatric Interview was used to identify a diagnosis of GAD. The Beck Depression Inventory was used to assess depressive symptom severity, and the Sheehan Disability Scale (SDS) was used to measure functional impairment.

Results

The prevalence of GAD was 11%. GAD was positively correlated with female gender ( P = .005), family history of psychiatric disorder ( P < .001), personal history of suicide attempt ( P = .019), and depressive symptoms ( P < .001). Based on the SDS, severe to very severe functional limitations were reported at work (56.4%), in social life (60.2%), and family life (73.1%) among GAD patients.

Conclusion

This study indicated a relatively high prevalence of GAD among primary care patients in Tunisia. Risk factors were also determined that may help to identify these patients. This information may help primary care physicians recognize this diagnosis and provide timely referrals to mental health professionals to prevent complications and maximize quality of life.
Using Visual Scene Displays With Young Children: An Evidence-Based Practice Synthesis
Authors: Patenaude Dana , McNaughton David , Liang Zhigao
Published: June 2024

We used the Council for Exceptional Children (CEC) Standards for evidence-based practices (EBP) in Special Education to evaluate the effectiveness research on the use of visual scene displays (VSDs) and video VSDs with young children with autism spectrum disorder and/or intellectual and developmental disability (ASD/IDD). VSDs and video VSDs are designed to support communication for individuals with speech or speech-related disabilities. In a high-tech VSD approach, images are programmed (on a tablet computer) with “hotspots” that produce speech when touched. Twelve of the 14 articles located for the review met all eight quality indicators as identified by the CEC Standards for evidence-based practices, and presented positive results for 37 of the 42 participants. We conclude that the use of VSDs and video VSDs can be considered an EBP in communication intervention for young children with ASD/IDD during social interaction activities commonly observed in early childhood settings.
Intergenerational Relations and Well-being Among Older Middle Eastern/Arab American Immigrants During the COVID-19 Pandemic
Authors: Sayed Linda , Alanazi Mohammed , Ajrouch Kristine J.
Published: June 2024

This study aimed to examine the consequences of COVID-19 socialization restrictions on familial and social support systems of older Middle Eastern/Arab immigrants in Michigan, home to the largest, most visible concentration of Middle Eastern/Arab Americans in the United States. Six focus group ( N = 45) interviews were conducted with Middle Eastern/Arab American immigrants aged 60 and older to assess difficulties faced during the pandemic as it related to familial, social, and medical care. Inductive analysis identified two major themes to advance meanings of intergenerational relations among older immigrants (a) the breakdown of family relations, which describes a shift in both the experience and expectations of intergenerational relations in Middle Eastern/Arab families; and (b) cultural sources of increased stress, illustrating how the pandemic interfered with valued family interactions to affect well-being. These findings indicated social and cultural sources of heightened stress linked to shifts in intergenerational relations among Middle Eastern/Arab American older immigrants.
‘Involuntary exit for personal reasons’ – A gendered critique of the business exit decision
Authors: Swail Janine , Marlow Susan
Published: June 2024

Women are more likely to exit from entrepreneurship for personal reasons; such exits are deemed uneventful and voluntary. We critically evaluate such assumptions by adopting a gendered critique to unpack the nature of such personal reasons arguing that they largely relate to the stress of accommodating incompatible caring/domestic and entrepreneurial labour demands. This, we argue, leads to forced voluntarism which, for many women, instigates conflicting notions of regret, relief and resentment. To explore these arguments, we draw upon in-depth interviews with 16 United Kingdom women entrepreneurs who had voluntarily exited from their ventures citing personal reasons. The evidence describes how women made sense of the decision-making process to exit from entrepreneurship questioning the voluntary nature of the exit decision. We conclude by noting how once again, gendered assumptions conceal the contradictory demands women encounter when trying to reconcile the needs of the household and of their ventures analysing the implications this has for how exit decisions are categorised and the false picture this presents of alleged voluntarism.
Clinical Guidelines for the Treatment of Depressive Disorders I. Definitions Prevalence Health Burden
Authors: Parikh Sagar V , Lam Raymond W ,   the CANMAT Depression Work Group
Published: June 2024

Background: The Canadian Psychiatric Association and the Canadian Network for Mood and Anxiety Treatments partnered to produce clinical guidelines for psychiatrists for the treatment of depressive disorders.

Methods: A standard guidelines development process was followed. Relevant literature was identified using a computerized Medline search supplemented by review of bibliographies. Operational criteria were used to rate the quality of scientific evidence, and the line of treatment recommendations included consensus clinical opinion. This section on "Definitions, Prevalence, and Health Burden” was 1 of 7 articles drafted and reviewed by clinicians. Revised drafts underwent national and international expert peer review.

Results: The 1-year prevalence rate of major depressive disorder (MDD) in Canada is 3.2% to 4.6%, similar to the rates in other countries. MDD frequently runs a chronic or recurrent course and carries high risks for mortality and morbidity. The significant economic costs and disability associated with depressive illness are reduced by effective treatment.

Conclusions: MDD is a prevalent medical condition that results in a significant health burden in the world. Vigorous efforts to improve diagnosis, treatment, and prevention are indicated to reduce the societal and personal costs of depressive disorders.
Role of bone single photon emission computed tomography/computed tomography in managing chronic fracture-related infections: Determining the depth of infection and avoiding unnecessary bone procedures
Authors: Lee Seung Hoo , Kim Min Bom , Jeon Yeong June
Published: June 2024

Purpose

Fracture-related infections (FRIs) encompass a broad range of infections associated with bone fractures; they remain a significant clinical challenge. Here, we aimed to investigate the viability of focusing on soft-tissue management in patients suspected of chronic FRI, who exhibit no significant bony uptake on bone single photon emission computed tomography (SPECT)/computed tomography (CT) scans.

Methods

Between January 2016 and January 2022, we managed 25 patients with chronic FRI or post-traumatic osteomyelitis using technetium 99m-methyl diphosphonate bone SPECT/CT to assess infection depth. Among them, 13 patients showing negligible bony uptake were included and categorized into two groups based on wound discharge reaching the bone/implant (Criteria 1, n = 6) or not (Criteria 2, n = 7).

Results

Patients in the Criteria 1 group were treated with antibiotics and soft tissue debridement without bony procedure. The average duration of antibiotic therapy was 6.7 weeks. Treatments were individualized, including implant changes, local flaps, skin grafts, and negative pressure wound therapy. No recurrence was reported in the mean follow-up of 21.3 months. Patients in the Criteria 2 group were treated with oral antibiotics (mean duration: 5.9 weeks) and daily wound dressings. No recurrence was reported in the mean follow-up of 26.0 months, and no surgical interventions were required.

Conclusion

This study demonstrates the feasibility of focusing on soft-tissue management in patients with chronic FRI showing minimal bony uptake on bone SPECT/CT. Our treatment protocol avoided unnecessary surgical bone procedures, resulting in successful clinical outcomes with no recurrences.
Safety Assessment of a Novel Intravenous Diclofenac Sodium Formulation Following 28-Day Repeated Administration in Wistar Rats
Authors: Bhatt Laxit K. , Shah Chitrang R. , Patel Jitendra H. , Rajwadi Viral I. , Dwivedi Pankaj , Patel Rajesh J. , Ranvir Ramchandra K. , Patel Harilal , Sundar Rajesh , Jain Mukul R.
Published: June 2024

These toxicity studies aimed to assess the safety and tolerability of a novel intravenous diclofenac sodium (37.5 mg/mL) formulation containing povidone K12 (80 mg/mL) as the key excipient in Wistar rats. This formulation was tested at doses of 3, 7, and 15 mg/kg/day and was administered daily for 28 days by intravenous route. Toxicokinetic estimation revealed a dose-proportional increase in plasma exposure to diclofenac. The formulation was well tolerated in males; however, mortality was observed in females (2/15) at the highest dose (15 mg/kg/day). Adverse gastrointestinal events related to NSAIDS and a few other treatment-related effects on clinical and anatomic pathology were noted at the 15 mg/kg/day dose, which normalized at the end of the 2-week recovery period. In addition, the excipient povidone K12 was present in a higher amount than the approved Inactive Ingredient Database (IID) limit in the proposed novel formulation. It was qualified through a separate 28-day repeated dose toxicity study by intravenous route in Wistar rats. Povidone K12 was found to be well tolerated and safe up to a dose of 165 mg/kg/day. No treatment-related adverse effects were observed in this study. In conclusion, repeated administration of a novel intravenous formulation containing diclofenac sodium was found to be safe up to the dose of 7 mg/kg/day in female rats and 15 mg/kg/day in male rats.
Risk of cancer-related distress by age in colorectal cancer survivors: The modulatory role of unmet support needs
Authors: Andreu Y. , Soto-Rubio A. , Picazo C. , Gil-Juliá B. , Fernández S. , Chulvi R.
Published: June 2024

To explore the modulating role of unmet support needs on the relationship between age and the prevalence of cancer-related distress in colorectal cancer (CRC) survivors. Two hundred and forty four participants completed the questionnaires; linear regression and odd ratios were calculated. Both the prevalence of needs and their interaction with age were predictors of cancer-related distress. The risk of significant clinically distress associated with physical and socio/family needs was high in both age subgroups. Higher risk of clinical distress was associated with life perspective, sexual, occupational and health care needs in the younger subgroup and with needs for specific support resources in the older subgroup. In reducing cancer-related distress, two key issues arise: (i) the importance of managing the persistent negative symptoms following CRC treatment in survivors of any age and (ii) the need for a differentiated attention to other care needs based on the survivor’s age.