bluuebunny/crossref_metadata_2025 huggingface dataset:
Metastatic malignant struma ovarii to the pituitary presenting as a sellar mass and responding to total thyroidectomy with adjuvant radioactive iodine therapy
Authors: Dissanayake Ashini , Liu Angela Y. , Gooderham Peter A. , MacKenzie-Feder Jessica
Published: June 2024

Malignant struma ovarii (MSO) is a rare ovarian teratoma composed primarily of thyroid tissue. Common sites of metastasis include peritoneum, bone, liver, lung, gastrointestinal tract and omentum. We present a woman in her 50s with a history of remote oophorectomy presenting with hypopituitarism and a 2.7 cm sellar mass. Trans-sphenoidal surgery for presumed pituitary macroadenoma achieved near total resection and resultant pathology surprisingly showed ectopic thyroid tissue. The patient acquired her ovarian pathology report from Southeast Asia which showed struma ovarii of the left ovary. The pituitary mass was thus determined to be a metastatic lesion from MSO. She underwent total thyroidectomy and radioactive iodine ablation therapy with good initial response and no regrowth of the tissue or emergence of distant metastases after 5 years of annual follow-up. To our knowledge, this is the first reported case of MSO to the pituitary.
Poppers maculopathy missed in a patient with cataract highlights the importance of preoperative optical coherence tomography
Authors: Kanda Mumta , Malik Mohsan , Miller Michael , Deshmukh Rajesh
Published: June 2024

Poppers maculopathy is a complication of alkyl nitrate (poppers) inhalation. It presents with non-specific symptoms and variable signs, which can make it difficult to diagnose. We present a case of coexisting cataract and poppers maculopathy in a patient. He had vague visual symptoms that were attributed entirely to his cataract and he went on to have cataract surgery. Suboptimal postoperative visual acuity and normal clinical examination triggered further investigation with spectral-domain optical coherence tomography (SD-OCT), after which poppers maculopathy was diagnosed. We highlight the importance of performing OCT in the preoperative assessment of a cataract patient, especially where the cataract is mild and may not fully account for symptoms. The patient showed complete visual recovery on drug cessation despite ongoing maculopathy on OCT scans.
Geographical altitude and stunting among children aged under 5 years in India
Authors: Phad Santosh Bhagwanrao , Siddiqui Laeek Ahemad , Khan Kacho Amir , Sinha Ratnesh , Rahman Mohammad Hifz Ur
Published: June 2024

Introduction

Previous studies have found positive associations between higher geographical altitude and increased risk of stunting in children under 5 years old, but little evidence exists on this relationship in the Indian context specifically. Chronic exposure to high altitudes can impair food security, healthcare access, oxygen delivery and nutrient absorption, potentially increasing malnutrition.

Objective

To investigate the association between geographical altitude and stunting among children aged under 5 years in India.

Methods

Using data from the 2015–2016 National Family Health Survey, logistic regression was conducted to estimate the relationship between altitude and stunting, adjusting for child, maternal and household characteristics. The analysis included over 167 555 children under 5 years old.

Results

Children at higher altitudes had a significantly greater risk of stunting. Those at >2000+ metres had 40% higher adjusted odds of stunting than children below 1000 metres. The altitude–stunting association was stronger among rural children.

Conclusions

This study provides robust evidence that higher geographical altitude is an important risk factor for stunting among young children in India, especially those in rural areas. Targeted interventions to improve food security, healthcare access and nutrition in high-altitude regions could help to mitigate the higher burden of stunting in these areas.
Development of a questionnaire for assessing the impact of children’s food marketing exposure on diet-related outcomes
Authors: Jindarattanaporn Nongnuch , Kelly Bridget , Punpuing Sureeporn , Phulkerd Sirinya
Published: June 2024

Introduction

The Government of Thailand has drafted legislation to protect children from the harmful impact of unhealthy food (including beverages) marketing. Local evidence on Thai children’s exposure to, and the impact of, this marketing is necessary to, first, support the adoption of this Law and, second, to contribute to assessing policy implementation and effectiveness. This study aimed to develop and validate a questionnaire for examining Thai children’s exposure to unhealthy food marketing and its impact on diet-related outcomes.

Materials and methods

To design the questionnaire, we first conceptualised the range of impacts of unhealthy food marketing on children’s diet-related outcomes based on published frameworks. These outcomes related to food brand loyalty, preference, purchase and consumption. We conducted a literature review to gather related questions used in earlier surveys to assess these outcomes. Using these questions, we assessed content validity with five experts. Face validity and reliability were assessed for 32 children. Validity was assessed using Content Validity Index (CVI) and Kappa statistics. Reliability was assessed using Cronbach’s alpha and intraclass correlation coefficients (ICC).

Results

We identified 15 survey questions that had been used to assess the outcomes of interest. The CVI of all questions was 1.0, indicating perfect agreement with each question’s relevance by the experts. Most questions were perceived to be easy to read and comprehend, suggesting face validity. Cronbach’s alpha and ICC of all questions were both 0.75, demonstrating internal consistency across responses to questions about, separately, brand loyalty, preferences, purchase and consumption.

Conclusion

The final 15-item questionnaire provides a valid and reliable survey instrument for measuring the impact of unhealthy food marketing on children’s diet-related outcomes. This instrument will be useful for gathering local evidence on the need for policy reform to protect children from unhealthy food marketing in Thailand. The instrument also provides a cost-effective approach for generating evidence in other jurisdictions to propel policy actions. This is a pilot study and the validity and reliability needs further testing after a larger-scale roll-out.
A study of self-adjointness, Lie analysis, wave structures, and conservation laws of the completely generalized shallow water equation
Authors: Ansari Ali R. , Jhangeer Adil , Imran Mudassar , Beenish  , Inc Mustafa
Published: June 2024

This article explores the analysis of the completely generalized Hirota–Satsuma–Ito equation through Lie symmetry analysis. The equation under consideration represents a more comprehensive form of the (2+1)-dimensional HSI equation, encompassing four additional second-order derivative terms: $$\Delta _{3}H_{\varpi \varpi }$$

Δ
3

H

ϖ
ϖ

, $$\Delta _{4}H_{\varpi \iota }$$

Δ
4

H

ϖ
ι

, $$\Delta _{3}H_{\varpi \varpi }$$

Δ
3

H

ϖ
ϖ

, $$\Delta _{4}H_{\varpi \iota }$$

Δ
4

H

ϖ
ι

, and $$\Delta _{6}H_{\iota \iota }$$

Δ
6

H

ι
ι

, emerging from the inclusion of second-order dissipative-type elements. We calculate the infinitesimal generators and determine the symmetry group for each generator using the Lie group invariance condition. Employing the conjugacy classes of the Abelian algebra, we transform the considered equation into an ordinary differential equation through similarity reduction. Subsequently, we solve these ordinary differential equations to derive closed-form solutions for the completely generalized Hirota–Satsuma–Ito equation under certain conditions. For other scenarios, we utilize the extended direct algebraic method to obtain soliton solutions. Furthermore, we rigorously calculated the conserved quantities corresponding to each symmetry generator, the conservation laws of the model are established using the multiplier approach. Additionally, we present the graphical representation of selected solutions for specific values of the physical parameters of the equation under scrutiny.
A note on Murasugi sum of trivially extendable relative trisections
Authors: Ghanwat Abhijeet , Pandit Suhas , Selvakumar A.
Published: June 2024

A relative trisection of a compact [Formula: see text]-manifold with connected boundary is said to be a Trivially Extendable (TE)-relative trisection if the boundary monodromy of the relative trisection is the identity map. We call a relative trisection diagram associated to a [Formula: see text]-relative trisection of a compact [Formula: see text]-manifold, a [Formula: see text]-relative trisection diagram. Due to a work of Castro and Ozbagci, there is a natural way to associate a trisection diagram to a [Formula: see text]-relative trisection diagram. In fact, every closed oriented [Formula: see text]-manifold can be realized as a [Formula: see text]-relative trisection.

In this paper, we study the notion of Murasugi sum of [Formula: see text]-relative trisection diagrams. We show that a trisection diagram associated to a Murasugi sum of two [Formula: see text]-relative trisection diagrams [Formula: see text] and [Formula: see text] is diffeomorphism and handle slide equivalent to the connected sum of the trisection diagrams associated to [Formula: see text] and [Formula: see text]. This implies that the closed oriented [Formula: see text]-manifold associated to the Murasugi sum of [Formula: see text]-relative trisection diagrams [Formula: see text] and [Formula: see text] is the connected sum of the closed [Formula: see text]-manifolds associated to [Formula: see text] and [Formula: see text]. Thus, the notion of Murasugi sum can be considered for closed [Formula: see text]-manifolds in terms of [Formula: see text]-relative trisections.

Finally, we discuss an external stabilization of a [Formula: see text]-relative trisection of a closed oriented [Formula: see text]-manifold. We prove that the trisection diagram associated to a stabilization of a [Formula: see text]-relative trisection diagram is diffeomorphism and handle slide equivalent to a stabilization of the trisection diagram associated to the [Formula: see text]-relative trisection diagram.
On two-bridge ribbon knots
Authors: Horigome Sayo , Ichihara Kazuhiro
Published: June 2024

We show that a two-bridge ribbon knot [Formula: see text] with [Formula: see text] and [Formula: see text] admits a symmetric union presentation with partial knot which is a two-bridge knot [Formula: see text]. Similar descriptions for all the other two-bridge ribbon knots are also given.
Free Vibration of Ogival Circular Arch
Authors: Lee Joon Kyu , Lee Byoung Koo , Yoon Hee Min , Lee Jong Cheon
Published: June 2024

This study aims to analyze the free vibration of an ogival arch in which two symmetrical arched shapes are discontinuously met at the mid-arc. Differential equations governing the free vibration of an ogival circular arch were derived, where the rotatory inertia and shear deformation effects are included. The governing equations were numerically solved to calculate natural frequencies and mode shapes. In numerical solution methods, the symmetric and anti-symmetric boundary conditions at the mid-arc were focused rather than the boundary conditions of supported end due to the discontinuity of the mid-arc. For the first time, the free vibration problem for discontinuous arches, such as ogival arches, is solved in this study. Calculation results of this study for natural frequencies are compared well with those of the finite element method. The effects of various arch parameters on natural frequencies were highlighted and discussed in detail.
Novel Design of Double Touch Plano-Convex MEMS Capacitive Pressure Sensor: Robust Design, Theoretical Modeling, Numerical Simulation and Performance Comparison
Authors: Rukshana Bi Gajula , Kumar Jindal Sumit
Published: June 2024

Due to advancements in Micro-Electro-Mechanical Systems (MEMS) fabrication technologies, researchers are incorporating numerous innovations in the design of capacitive pressure sensors (CPS). This work aims to present a novel CPS design using a plano-convex substrate to enhance the capacitance and sensitivity. Diaphragm deflection occurs due to its elastic property when pressure is applied to the diaphragm. This deflection reduces the distance between the diaphragm and substrate, thereby remarkably increasing capacitance. The Plano-Convex design offers added advantages of increased contact area between the diaphragm and substrate under applied pressure, hence significantly enhancing sensor sensitivity and range. More efficient and miniaturized CPSs are in high demand in medical instrumentation, aerospace, aviation, power plants and automotive industries. This work presents all the required mathematical calculations, modeling and simulations to support the proposed design. The diaphragm deflection simulation concerning pressure is conducted using COMSOL Multiphysics, while MATLAB is employed for analytical simulations related to changes in capacitance and capacitive sensitivity.
Intraovarian Injection of Lyophilized Platelet-Rich Plasma in Patients with Poor Ovarian Response
Authors: Karino Aya , Matsubayashi Hidehiko , Saito Saki , Ishibashi Yurino , Usui Kento , Doshida Masakazu , Takeuchi Takumi , Suzuki Yosuke , Ohara Yasuhiro , Ishikawa Tomomoto
Published: June 2024

Objective: To demonstrate the effect of intraovarian injection of lyophilized platelet-rich plasma (PRP) in poor ovarian responders.

Methods: This retrospective cohort study evaluated data for 262 women who underwent intraovarian injection of lyophilized PRP at two private fertility clinics. Women treated from December 2021 to September 2022 and who met the Bologna criteria were included. Intraovarian injection of lyophilized PRP prepared from 50[Formula: see text]mL of autologous peripheral blood was followed by two centrifugations and activation with 2% CaCl2, in which cells were removed with a filter and stored at room temperature. The differences in the follicle-stimulating hormone and antral follicle count (AFC), and the number of retrieved oocytes, mature oocytes, fertilized embryos, cleavage embryos, and blastocysts were measured before and 3 months after a single injection. A biological pregnancy with embryos generated after injection was also examined.

Results: Patients’ median age and anti-Mullerian hormone (AMH) levels were 44 years and 0.38[Formula: see text]ng/mL, respectively. The number of retrieved oocytes or mature oocytes and fertilized embryos or cleavage embryos was higher after injection. The number of cleavage embryos in women [Formula: see text] years significantly increased after injection. Of the 262 patients who underwent intraovarian injection of lyophilized PRP, 71 underwent embryo transfers using embryos obtained 3 months after injection, and 14 had biological pregnancies. Seven of these patients were in their 40s. Out of 14, one had a live birth, six are ongoing pregnancies, six had chemical abortions, and one had a miscarriage.

Conclusions: Intraovarian injection of lyophilized PRP improved egg retrieval results, especially in patients older than 40 years. Furthermore, this treatment may contribute to biological pregnancy in patient with poor ovarian response but needs to be further investigated in more cases in the future.
Evolutionary Seeding of Diverse Structural Design Solutions via Topology Optimization
Authors: Xie Yue , Pinskier Josh , Wang Xing , Howard David
Published: June 2024

Topology optimization is a powerful design tool in structural engineering and other engineering problems. The design domain is discretized into elements, and a finite element method model is iteratively solved to find the element that maximizes the structure's performance. Although gradient-based solvers have been used to solve topology optimization problems, they may be susceptible to suboptimal solutions or difficulty obtaining feasible solutions, particularly in non-convex optimization problems. The presence of non-convexities can hinder convergence, leading to challenges in achieving the global optimum. With this in mind, we discuss in this article the application of the quality diversity approach to topological optimization problems. Quality diversity (QD) algorithms have shown promise in the research field of optimization and have many applications in engineering design, robotics, and games. MAP-Elites is a popular QD algorithm used in robotics. In soft robotics, the MAP-Elites algorithm has been used to optimize the shape and control of soft robots, leading to the discovery of new and efficient motion strategies. This article introduces an approach based on MAP-Elites to provide diverse designs for structural optimization problems. Three fundamental topology optimization problems are used for experimental testing, and the results demonstrate the ability of the proposed algorithm to generate diverse, high-performance designs for those problems. Furthermore, the proposed algorithm can be a valuable engineering design tool capable of creating novel and efficient designs.
Supplementary Fig. S2 from Whole-Genome DNA Methylation Profiling of Intrahepatic Cholangiocarcinoma Reveals Prognostic Subtypes with Distinct Biological Drivers
Authors: Liao Haotian , Chen Xing , Wang Haichuan , Lin Youpei , Chen Lu , Yuan Kefei , Liao Mingheng , Jiang Hanyu , Peng Jiajie , Wu Zhenru , Huang Jiwei , Li Jiaxin , Zeng Yong
Published: June 2024

Supplementary Fig. S2
Supplementary Table S1 from ACSL4-Mediated Membrane Phospholipid Remodeling Induces Integrin β1 Activation to Facilitate Triple-Negative Breast Cancer Metastasis
Authors: Qiu Yuxiang , Wang Xing , Sun Yan , Jin Ting , Tang Rui , Zhou Xinyue , Xu Ming , Gan Yubi , Wang Rui , Luo Haojun , Liu Manran , Tang Xi
Published: June 2024

Supplementary Table S1
Supplementary Table S7 from The Deep Learning Framework iCanTCR Enables Early Cancer Detection Using the T-cell Receptor Repertoire in Peripheral Blood
Authors: Cai Yideng , Luo Meng , Yang Wenyi , Xu Chang , Wang Pingping , Xue Guangfu , Jin Xiyun , Cheng Rui , Que Jinhao , Zhou Wenyang , Pang Boran , Xu Shouping , Li Yu , Jiang Qinghua , Xu Zhaochun
Published: June 2024

Details of performance comparison results
Table S11 from Cigarette Smoking and E-cigarette Use Induce Shared DNA Methylation Changes Linked to Carcinogenesis
Authors: Herzog Chiara , Jones Allison , Evans Iona , Raut Janhavi R. , Zikan Michal , Cibula David , Wong Andrew , Brenner Hermann , Richmond Rebecca C. , Widschwendter Martin
Published: June 2024

Supplementary Table 11
Figure 4 from Cigarette Smoking and E-cigarette Use Induce Shared DNA Methylation Changes Linked to Carcinogenesis
Authors: Herzog Chiara , Jones Allison , Evans Iona , Raut Janhavi R. , Zikan Michal , Cibula David , Wong Andrew , Brenner Hermann , Richmond Rebecca C. , Widschwendter Martin
Published: June 2024

Impact of e-cigarette and smokeless use on cell type–specific epigenetic smoking signatures. A, Mean beta values (corrected) in each of the four sets in saliva samples of never or current smokers or e-cigarette users, corrected for cell type–specific effects. B, AUC of corrected values in each of the four sets comparing smokers or e-cigarette users with controls in the e-cigarette use dataset. C, Mean beta values in each of the four sets in never smokers (control) or smokers, stratified by categorial smoking duration information. D, Mean beta values in each of the four sets in never smokers (control) or e-cigarette users, stratified by categorial e-cigarette duration information. The legend is identical to C. E, Mean beta values (corrected) in each of the four sets in saliva samples of current nonsmokers (prior smoking history not known), smokeless tobacco users, or smokers in the smokeless tobacco use set. F, AUC of corrected values in each of the four sets of CpGs comparing nonsmokers with smokeless tobacco users or smokers in the smokeless tobacco use set. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 in Wilcoxon test compared with relevant controls (never or nonsmokers, respectively, for A, C, D, and E).
Figure 2 from Cigarette Smoking and E-cigarette Use Induce Shared DNA Methylation Changes Linked to Carcinogenesis
Authors: Herzog Chiara , Jones Allison , Evans Iona , Raut Janhavi R. , Zikan Michal , Cibula David , Wong Andrew , Brenner Hermann , Richmond Rebecca C. , Widschwendter Martin
Published: June 2024

Combined methylation scores of CpGs in the four sets and annotation. A, Association of mean methylation β values in each of the sets described in Fig. 1C with immune cell proportion in buccal and cervical samples and lymphoid proportion in blood samples in the discovery set. B, Venn Diagram of genes associated with CpGs in each of the four smoking-associated sets of CpG indicates little overlap between involved genes. C–F, Gene ontology (C–E) and Reactome pathway enrichment (F) for the four sets of smoking-associated CpGs reveals different pathways.
Racialized Economic Segregation and Treatment and Outcomes of Small Cell Lung Cancer
Authors: Bekele Bayu B. , Lian Min , Shrestha Pratibha , Nabi Oumarou , Kozower Benjamin , Baggstrom Maria Q. , Liu Ying
Published: June 2024

Background:

Little is known about the role of residential segregation in the treatment and outcomes of small cell lung cancer (SCLC), a highly recalcitrant disease, among non-Hispanic White (NHW) and non-Hispanic Black (NHB) patients.

Methods:

We used the Surveillance, Epidemiology, and End Results database to identify men and women diagnosed with SCLC from January 2007 to December 2015 (n = 38,393). An Index of Concentration at the Extremes was computed to measure county-level racialized economic segregation and categorized into Quartile 1 (most privileged: highest concentration of high-income NHW residents) through Quartile 4 (least privileged: highest concentration of low-income NHB residents). Multilevel logistic regression was used to estimate the ORs for extensive-stage diagnosis and nonadherence to guideline-recommended treatment. HRs for lung cancer–specific and overall mortalities were computed using multilevel Cox regression.

Results:

Patients in the least privileged counties had higher risks of nonadherence to guideline-recommended treatment [OR = 1.23; 95% confidence interval (CI): 1.08–1.40; Ptrend < 0.01], lung cancer–specific mortality (HR = 1.08; 95% CI: 1.04–1.12; Ptrend < 0.01), and all-cause mortality (HR = 1.13; 95% CI: 1.09–1.17; Ptrend < 0.0001) compared with patients in the most privileged counties. Adjustment for treatment did not significantly reduce the association with mortality. These associations were comparable between NHB and NHW patients. Segregation was not significantly associated with extensive-stage diagnosis.

Conclusions:

The results suggest that living in the neighborhoods with higher proportions of low-income households and Black residents had adverse impacts on stage-appropriate treatment of and survival from SCLC.

Impact:

This highlights the need for improving the access to quality lung cancer care in the less privileged neighborhoods.
Spatially Informed Gene Signatures for Response to Immunotherapy in Melanoma
Authors: Aung Thazin N. , Warrell Jonathan , Martinez-Morilla Sandra , Gavrielatou Niki , Vathiotis Ioannis , Yaghoobi Vesal , Kluger Harriet M. , Gerstein Mark , Rimm David L.
Published: June 2024

Purpose:

We aim to improve the prediction of response or resistance to immunotherapies in patients with melanoma. This goal is based on the hypothesis that current gene signatures predicting immunotherapy outcomes show only modest accuracy due to the lack of spatial information about cellular functions and molecular processes within tumors and their microenvironment.

Experimental Design:

We collected gene expression data spatially from three cellular compartments defined by CD68+ macrophages, CD45+ leukocytes, and S100B+ tumor cells in 55 immunotherapy-treated melanoma specimens using Digital Spatial Profiling–Whole Transcriptome Atlas. We developed a computational pipeline to discover compartment-specific gene signatures and determine if adding spatial information can improve patient stratification.

Results:

We achieved robust performance of compartment-specific signatures in predicting the outcome of immune checkpoint inhibitors in the discovery cohort. Of the three signatures, the S100B signature showed the best performance in the validation cohort (N = 45). We also compared our compartment-specific signatures with published bulk signatures and found the S100B tumor spatial signature outperformed previous signatures. Within the eight-gene S100B signature, five genes (PSMB8, TAX1BP3, NOTCH3, LCP2, and NQO1) with positive coefficients predict the response, and three genes (KMT2C, OVCA2, and MGRN1) with negative coefficients predict the resistance to treatment.

Conclusions:

We conclude that the spatially defined compartment signatures utilize tumor and tumor microenvironment–specific information, leading to more accurate prediction of treatment outcome, and thus merit prospective clinical assessment.
Table S5 from A Novel Dual ATM/DNA-PK Inhibitor, XRD-0394, Potently Radiosensitizes and Potentiates PARP and Topoisomerase I Inhibitors
Authors: Gilmer Tona M. , Lai Chun-Hsiang , Guo Kexiao , Deland Katherine , Ashcraft Kathleen A. , Stewart Amy E. , Wang Yaode , Fu Jianmin , Wood Kris C. , Kirsch David G. , Kastan Michael B.
Published: June 2024

Clonogenic survival treated with ATMi, DNA-PKi, the combination and XRD-0394
Kinase Panel 1 from A Novel Dual ATM/DNA-PK Inhibitor, XRD-0394, Potently Radiosensitizes and Potentiates PARP and Topoisomerase I Inhibitors
Authors: Gilmer Tona M. , Lai Chun-Hsiang , Guo Kexiao , Deland Katherine , Ashcraft Kathleen A. , Stewart Amy E. , Wang Yaode , Fu Jianmin , Wood Kris C. , Kirsch David G. , Kastan Michael B.
Published: June 2024

Results of Kinase Panel Screen-1
Figure S2 from A Novel Dual ATM/DNA-PK Inhibitor, XRD-0394, Potently Radiosensitizes and Potentiates PARP and Topoisomerase I Inhibitors
Authors: Gilmer Tona M. , Lai Chun-Hsiang , Guo Kexiao , Deland Katherine , Ashcraft Kathleen A. , Stewart Amy E. , Wang Yaode , Fu Jianmin , Wood Kris C. , Kirsch David G. , Kastan Michael B.
Published: June 2024

toxicity and KEAP1 cell lines
Supplemental Figure S4 from Mitochondrial and Cytosolic One-Carbon Metabolism Is a Targetable Metabolic Vulnerability in Cisplatin-Resistant Ovarian Cancer
Authors: Wallace-Povirk Adrianne , O'Connor Carrie , Dekhne Aamod S. , Bao Xun , Nayeen Md. Junayed , Schneider Mathew , Katinas Jade M. , Wong-Roushar Jennifer , Kim Seongho , Polin Lisa , Li Jing , Back Jessica B. , Dann Charles E. , Gangjee Aleem , Hou Zhanjun , Matherly Larry H.
Published: June 2024

This figure shows generation of SKOV3 SHMT1 and SHMT2 knockdown (KD) cell lines.
Supplementary Data from 89Zr-ImmunoPET for the Specific Detection of EMP2-Positive Tumors
Authors: Chan Ann M. , Olafsen Tove , Tsui Jessica , Salazar Felix B. , Aguirre Brian , Zettlitz Kirstin A. , Condro Michael , Wu Anna M. , Braun Jonathan , Gordon Lynn K. , Ashki Negin , Whitelegge Julian , Xu Shili , Ikotun Oluwatayo , Lee Jason Thanh , Wadehra Madhuri
Published: June 2024

Supplemental Figure Legends
Supplementary Table 7 from Thio-2 Inhibits Key Signaling Pathways Required for the Development and Progression of Castration-resistant Prostate Cancer
Authors: Neeb Antje , Figueiredo Ines , Bogdan Denisa , Cato Laura , Stober Jutta , Jiménez-Vacas Juan M. , Gourain Victor , Lee Irene I. , Seeger Rebecca , Muhle-Goll Claudia , Gurel Bora , Welti Jonathan , Nava Rodrigues Daniel , Rekowski Jan , Qiu Xintao , Jiang Yija , Di Micco Patrizio , Mateos Borja , Bielskutė Stasė , Riisnaes Ruth , Ferreira Ana , Miranda Susana , Crespo Mateus , Buroni Lorenzo , Ning Jian , Carreira Suzanne , Bräse Stefan , Jung Nicole , Gräßle Simone , Swain Amanda , Salvatella Xavier , Plymate Stephen R. , Al-Lazikani Bissan , Long Henry W. , Yuan Wei , Brown Myles , Cato Andrew C.B. , de Bono Johann S. , Sharp Adam
Published: June 2024

Synthego BAG-1 sgRNA for BAG-1 CRISPR knockout clones.
Supplementary Figure 4 from Thio-2 Inhibits Key Signaling Pathways Required for the Development and Progression of Castration-resistant Prostate Cancer
Authors: Neeb Antje , Figueiredo Ines , Bogdan Denisa , Cato Laura , Stober Jutta , Jiménez-Vacas Juan M. , Gourain Victor , Lee Irene I. , Seeger Rebecca , Muhle-Goll Claudia , Gurel Bora , Welti Jonathan , Nava Rodrigues Daniel , Rekowski Jan , Qiu Xintao , Jiang Yija , Di Micco Patrizio , Mateos Borja , Bielskutė Stasė , Riisnaes Ruth , Ferreira Ana , Miranda Susana , Crespo Mateus , Buroni Lorenzo , Ning Jian , Carreira Suzanne , Bräse Stefan , Jung Nicole , Gräßle Simone , Swain Amanda , Salvatella Xavier , Plymate Stephen R. , Al-Lazikani Bissan , Long Henry W. , Yuan Wei , Brown Myles , Cato Andrew C.B. , de Bono Johann S. , Sharp Adam
Published: June 2024

Mouse organ hematoxylin and eosin, and BAG-1 immunohistochemistry, in BAG-1 knockout mice.
Figure S5 from Evaluation of the Role of AXL in Fusion-positive Pediatric Rhabdomyosarcoma Identifies the Small-molecule Inhibitor Bemcentinib (BGB324) as Potent Chemosensitizer
Authors: Danielli Sara G. , Wurth Jakob , Morice Sarah , Kisele Samanta , Surdez Didier , Delattre Olivier , Bode Peter K. , Wachtel Marco , Schäfer Beat W.
Published: June 2024

Effect of in vivo treatment on mouse weight.
Supplementary Figure S6 from Systematic Characterization of p53-Regulated Long Noncoding RNAs across Human Cancers Reveals Remarkable Heterogeneity among Different Tumor Types
Authors: Regunath Kausik , Fomin Vitalay , Liu Zhaoqi , Wang Pingzhang , Hoque Mainul , Tian Bin , Rabadan Raul , Prives Carol
Published: June 2024

Supplementary Figure S6 shows amplified and deleted lncRNAs from TCGA cancer datasets that are correlated with better or worse survival
Supplementary Figure S13 from Serotonin Receptor HTR2B Facilitates Colorectal Cancer Metastasis via CREB1–ZEB1 Axis–Mediated Epithelial–Mesenchymal Transition
Authors: Li Tao , Wei Lei , Zhang Xin , Fu Bin , Zhou Yunjiang , Yang Mengdi , Cao Mengran , Chen Yaxin , Tan Yingying , Shi Yongwei , Wu Leyin , Xuan Chenyuan , Du Qianming , Hu Rong
Published: June 2024

This file contains Supplementary Figure S13 and its legend.
Table S3 from Circulating miRNA Signature Predicts Cancer Incidence in Lynch Syndrome—A Pilot Study
Authors: Sievänen Tero , Jokela Tiina , Hyvärinen Matti , Korhonen Tia-Marje , Pylvänäinen Kirsi , Mecklin Jukka-Pekka , Karvanen Juha , Sillanpää Elina , Seppälä Toni T. , Laakkonen Eija K.
Published: June 2024

Table S3: Differentially expressed c-miRs between cancer-free LS carriers and healthy non-carrier controls. Lasso-selected c- miRs used for computing c-miR risk sum score are bolded. SE = standard error, Wald = Wald-test statistic, FDR = False discovery rate.
Data from Translational and Therapeutic Evaluation of RAS-GTP Inhibition by RMC-6236 in RAS-Driven Cancers
Authors: Jiang Jingjing , Jiang Lingyan , Maldonato Benjamin J. , Wang Yingyun , Holderfield Matthew , Aronchik Ida , Winters Ian P. , Salman Zeena , Blaj Cristina , Menard Marie , Brodbeck Jens , Chen Zhe , Wei Xing , Rosen Michael J. , Gindin Yevgeniy , Lee Bianca J. , Evans James W. , Chang Stephanie , Wang Zhican , Seamon Kyle J. , Parsons Dylan , Cregg James , Marquez Abby , Tomlinson Aidan C.A. , Yano Jason K. , Knox John E. , Quintana Elsa , Aguirre Andrew J. , Arbour Kathryn C. , Reed Abby , Gustafson W. Clay , Gill Adrian L. , Koltun Elena S. , Wildes David , Smith Jacqueline A.M. , Wang Zhengping , Singh Mallika
Published: June 2024

Abstract
RAS-driven cancers comprise up to 30% of human cancers. RMC-6236 is a RAS(ON) multi-selective noncovalent inhibitor of the active, GTP-bound state of both mutant and wild-type variants of canonical RAS isoforms with broad therapeutic potential for the aforementioned unmet medical need. RMC-6236 exhibited potent anticancer activity across RAS-addicted cell lines, particularly those harboring mutations at codon 12 of KRAS. Notably, oral administration of RMC-6236 was tolerated in vivo and drove profound tumor regressions across multiple tumor types in a mouse clinical trial with KRASG12X xenograft models. Translational PK/efficacy and PK/PD modeling predicted that daily doses of 100 mg and 300 mg would achieve tumor control and objective responses, respectively, in patients with RAS-driven tumors. Consistent with this, we describe here objective responses in two patients (at 300 mg daily) with advanced KRASG12X lung and pancreatic adenocarcinoma, respectively, demonstrating the initial activity of RMC-6236 in an ongoing phase I/Ib clinical trial (NCT05379985).
Significance:
The discovery of RMC-6236 enables the first-ever therapeutic evaluation of targeted and concurrent inhibition of canonical mutant and wild-type RAS-GTP in RAS-driven cancers. We demonstrate that broad-spectrum RAS-GTP inhibition is tolerable at exposures that induce profound tumor regressions in preclinical models of, and in patients with, such tumors.

This article is featured in Selected Articles from This Issue, p. 897
Figure S3 from The ω-3 Polyunsaturated Fatty Acid Docosahexaenoic Acid Enhances NK-Cell Antitumor Effector Functions
Authors: Wu Shuting , Peng Hongyan , Li Songyang , Huang Lanlan , Wang Xiangyu , Li Yana , Liu Yongjie , Xiong Peiwen , Yang Qinglan , Tian Kunpeng , Wu Weiru , Pu Rongxi , Lu Xiulan , Xiao Zhenghui , Yang Jian , Zhong Zhaoyang , Gao Yuan , Deng Yafei , Deng Youcai
Published: June 2024

Figure. S3. DHA-enriched diet withdrawal at the beginning of tumour injection had a decreased antitumour effect.
Supplemental Table 6 from Randomized Phase II Study Evaluating the Addition of Pembrolizumab to Radium-223 in Metastatic Castration-resistant Prostate Cancer
Authors: Choudhury Atish D. , Kwak Lucia , Cheung Alexander , Allaire Kathryn M. , Marquez Jaqueline , Yang David D. , Tripathi Abhishek , Kilar Jacqueline M. , Flynn Meredith , Maynard Brianna , Reichel Rebecca , Pace Amanda F. , Chen Brandon K. , Van Allen Eliezer M. , Kilbridge Kerry , Wei Xiao X. , McGregor Bradley A. , Pomerantz Mark M. , Bhatt Rupal S. , Sweeney Christopher J. , Bubley Glenn J. , Jacene Heather A. , Taplin Mary-Ellen , Huang Franklin W. , Harshman Lauren C. , Fong Lawrence
Published: June 2024

Time to event (months) clinical endpoints by baseline and change in CD4+/FoxP3- (effector) proportion, CD8+ proportion, CD8+ / CD4+FoxP3- (Treg) ratio
Supplemental Table 5 from Randomized Phase II Study Evaluating the Addition of Pembrolizumab to Radium-223 in Metastatic Castration-resistant Prostate Cancer
Authors: Choudhury Atish D. , Kwak Lucia , Cheung Alexander , Allaire Kathryn M. , Marquez Jaqueline , Yang David D. , Tripathi Abhishek , Kilar Jacqueline M. , Flynn Meredith , Maynard Brianna , Reichel Rebecca , Pace Amanda F. , Chen Brandon K. , Van Allen Eliezer M. , Kilbridge Kerry , Wei Xiao X. , McGregor Bradley A. , Pomerantz Mark M. , Bhatt Rupal S. , Sweeney Christopher J. , Bubley Glenn J. , Jacene Heather A. , Taplin Mary-Ellen , Huang Franklin W. , Harshman Lauren C. , Fong Lawrence
Published: June 2024

Summary of genetic findings from targeted sequencing of ctDNA and whole exome sequencing (WES) from bone biopsy specimen.
Data from An IRF2-Expressing Oncolytic Virus Changes the Susceptibility of Tumor Cells to Antitumor T Cells and Promotes Tumor Clearance
Authors: Shao Lulu , Srivastava Rashmi , Delgoffe Greg M. , Thorne Stephen H. , Sarkar Saumendra N.
Published: June 2024

Abstract
IFN regulatory factor 1 (IRF1) can promote antitumor immunity. However, we have shown previously that in the tumor cell, IRF1 can promote tumor growth, and IRF1-deficient tumor cells exhibit severely restricted tumor growth in several syngeneic mouse tumor models. Here, we investigate the potential of functionally modulating IRF1 to reduce tumor progression and prolong survival. Using inducible IRF1 expression, we established that it is possible to regulate IRF1 expression to modulate tumor progression in established B16-F10 tumors. Expression of IRF2, which is a functional antagonist of IRF1, downregulated IFNγ-induced expression of inhibitory ligands, upregulated MHC-related molecules, and slowed tumor growth and extended survival. We characterized the functional domain(s) of IRF2 needed for this antitumor activity, showing that a full-length IRF2 was required for its antitumor functions. Finally, using an oncolytic vaccinia virus as a delivery platform, we showed that IRF2-expressing vaccinia virus suppressed tumor progression and prolonged survival in multiple tumor models. These results suggest the potency of targeting IRF1 and using IRF2 to modulate immunotherapy.
Data from Randomized Phase II Study Evaluating the Addition of Pembrolizumab to Radium-223 in Metastatic Castration-resistant Prostate Cancer
Authors: Choudhury Atish D. , Kwak Lucia , Cheung Alexander , Allaire Kathryn M. , Marquez Jaqueline , Yang David D. , Tripathi Abhishek , Kilar Jacqueline M. , Flynn Meredith , Maynard Brianna , Reichel Rebecca , Pace Amanda F. , Chen Brandon K. , Van Allen Eliezer M. , Kilbridge Kerry , Wei Xiao X. , McGregor Bradley A. , Pomerantz Mark M. , Bhatt Rupal S. , Sweeney Christopher J. , Bubley Glenn J. , Jacene Heather A. , Taplin Mary-Ellen , Huang Franklin W. , Harshman Lauren C. , Fong Lawrence
Published: June 2024

Abstract
The checkpoint immunotherapeutic pembrolizumab induces responses in a small minority of patients with metastatic castration-resistant prostate cancer (mCRPC). Radium-223 (R223) may increase immunogenicity of bone metastases and increase pembrolizumab (P) activity. In a randomized phase II study, we assessed the effect of R223+P compared with R223 on tumor immune infiltration, safety, and clinical outcomes in patients with mCRPC. The primary endpoint was differences in CD4+ and CD8+ T-cell infiltrate in 8-week versus baseline bone metastasis biopsies; secondary endpoints were safety, radiographic progression-free survival (rPFS), and overall survival (OS). Of the 42 treated patients (29 R223+P, 13 R223), 18 R223+P and 8 R223 patients had evaluable paired tumor biopsies. Median fold-change of CD4+ T cells was −0.7 (range: −9.3 to 4.7) with R223+P and 0.1 (−11.1 to 3.7) with R223 (P = 0.66); for CD8+ T cells, median fold-change was −0.6 (−7.4 to 5.3) with R223+P and −1.3 (−3.1 to 4.8) with R223 (P = 0.66). Median rPFS and OS was 6.1 (95% confidence interval: 2.7–11.0) and 16.9 months [12.7–not reached (NR)], respectively, with R223+P and 5.7 (2.6–NR) and 16.0 (9.0–NR), respectively, with R223. Although R223+P was well tolerated with no unexpected toxicity, the combination did not improve efficacy. High-dimensional flow cytometry demonstrated minimal immune modulation with R223, whereas R223+P induced CTLA-4 expression on circulating CD4+ T cells. Clinical responders possessed lower circulating frequencies of Ki67+ T and myeloid cells at baseline and higher circulating frequencies of TIM-3+ T and myeloid cells by week 9. Although R223+P did not induce T-cell infiltration into the tumor microenvironment, exhaustion of induced peripheral T-cell immune responses may dampen the combination's clinical activity.
Pitfalls in Gynecological Cytology: Review of the Common and Less Frequent Entities in Pap Test
Authors: Vrdoljak-Mozetič Danijela , Štemberger-Papić Snježana , Verša Ostojić Damjana , Rubeša Roberta , Klarić Marko , Eminović Senija
Published: June 2024

Background: Pitfalls in Pap test could be defined as false positive, false negative, or underdiagnosed results which can lead to unnecessary diagnostic procedures or delayed and inadequate treatment. It can be a consequence of misinterpretation of certain morphological entities which are described in this paper. Summary: The paper presents an overview of the morphological features and look-alikes of the common sources of pitfalls such as atrophy, repair, intrauterine device change, tubal metaplasia, hyperchromatic crowded groups, and radiation changes. Rare causes of pitfalls such as Arias-Stella changes, pemphigus, tumor diathesis per se, rare types of cervical cancer, including verrucous and papillary squamous cell cancer, gastric type, and endometrioid adenocarcinoma are also described. Key Messages: The awareness of pitfalls in cervical cytology is important for cytopathologists and clinicians to avoid future errors. Review of Pap tests with erroneous diagnosis is important for quality control in cytology laboratory, and it must be considered an educational- and experience-building procedure. Cytopathologist should not pull back in significant diagnoses, especially in human papillomavirus-negative cases.
Utilizing ChatGPT to Facilitate Referrals for Fetal Echocardiography
Authors: Gordin Kopylov Lital , Goldrat Itai , Maymon Ron , Svirsky Ran , Wiener Yifat , Klang Eyal
Published: June 2024

Introduction: OpenAI’s GPT-4 (artificial intelligence [AI]) is being studied for its use as a medical decision support tool. This research examines its accuracy in refining referrals for fetal echocardiography (FE) to improve early detection and outcomes related to congenital heart defects (CHDs). Methods: Past FE data referred to our institution were evaluated separately by pediatric cardiologist, gynecologist (human experts [experts]), and AI, according to established guidelines. We compared experts and AI’s agreement on referral necessity, with experts addressing discrepancies. Results: Total of 59 FE cases were addressed retrospectively. Cardiologist, gynecologist, and AI recommended performing FE in 47.5%, 49.2%, and 59.0% of cases, respectively. Comparing AI recommendations to experts indicated agreement of around 80.0% with both experts (p < 0.001). Notably, AI suggested more echocardiographies for minor CHD (64.7%) compared to experts (47.1%), and for major CHD, experts recommended performing FE in all cases (100%) while AI recommended in majority of cases (90.9%). Discrepancies between AI and experts are detailed and reviewed. Conclusions: The evaluation found moderate agreement between AI and experts. Contextual misunderstandings and lack of specialized medical knowledge limit AI, necessitating clinical guideline guidance. Despite shortcomings, AI’s referrals comprised 65% of minor CHD cases versus experts 47%, suggesting its potential as a cautious decision aid for clinicians.
Evaluating machine learning models for precipitation prediction in Casablanca City
Authors: Tricha Abdelouahed , Moussaid Laila
Published: June 2024

Accurate precipitation forecasting is a vital task for many domains, such as agriculture, water management, flood prevention, and crop yield estimation. The use of machine learning (ML) approaches has improved precipitation forecasting accuracy, exhibiting promising results in capturing the intricate connections between various meteorological variables and precipitation patterns. However, given the vast array of available ML models, a comparative analysis is imperative for identifying the most effective models for precipitation prediction. This study aims to examine the capacities of ML algorithms to forecast precipitation based on weather data for the city of Casablanca, Morocco, which faces challenges in water management and climate change adaptation. Eight different ML models’ performances are compared: linear regression, polynomial regression, K-nearest neighbors (KNN), support vector machine (SVM), decision tree (DT), random forest (RF), XGBoost, and an ensemble learning model. These models are evaluated based on their mean absolute error (MAE), mean squared error (MSE), and R-squared (R2 ) value to determine their effectiveness. The study showcases the potential of ML models in predicting precipitation by utilizing meteorological parameters such as temperature, humidity, wind speed, and pressure.
Lifetime (Bx) improvement of PV inverter using Si-SiC H-IGBT/Diode: a reliability approach
Authors: Ramavath Muneeshwar , Puvvula Venkata Rama Krishna
Published: June 2024

Technological advancements have made it possible to harness the power of renewable energy sources. The efficiency of power electronic devices has increased to almost 98%. In order to reduce the risks of failure and maintain the operation of photovoltaic (PV)-based energy converters, reliable devices are needed. Due to the increasing number of wide-bandgap silicon in electronic converters, the need for more efficient and reliable devices has become more prevalent. However, the cost of these devices is a major issue. Hence, in this work extensive analysis of hybrid silicon (Si)-IGBT and silicon carbide (SiC) antiparallel Diode (H-IGBT/Diode) based PV inverter is proposed to improve the lifetime (Bx). A reliability oriented lifetime assessment is performed on a test case of single stage three kilowatt photovoltaic inverter with 600 V/30 A hybrid switch. Long term mission profile for one year is considered for evaluation at B. V. Raju Institute of Technology (BVRIT), Telangana, India. Finally, B10 lifetime is calculated, comparison analysis is presented between conventional Si-IGBT and proposed Si-SiC H-IGBT/Diode. The results of the study revealed that the H-IGBT exhibited a significant increase in PV inverter reliability.
Impacts of Eu2+ -doped K3LuSi2O7 phosphor and a scattering particle on conventional white light emitting diodes
Authors: Duy Le Doan , Thai Nguyen Le , Cong Pham Hong , Tran Thinh Cong
Published: June 2024

The K3LuSi2O7 phosphor doping Eu2+ rare-earth ions (KLS:Eu) was reported to possess broad emission band from near-ultraviolet to nearinfrared. Additionally, this phosphor showed a wide absorption band of 250-600 nm, allowing it to be excited by blue-light chip of 460 nm, making it one of the suitable phosphor materials for a light emitting diode (LED). Besides, the scattering particle material CaCO3 is incorporated into the yellow phosphor layer to serve the scattering-enhancement purpose. The combination of both materials aims at accomplishing improvements in performance of commercial LED package. The concentration of KLS:Eu is constant while that of CaCO3 is modified. As a result, the scattering factor is regulated and become the key factor influencing the optical outputs of the simulated LED. The increasing CaCO3 concentration enhances the phosphor scattering efficiency of light, helping to improve the lumen output and color-temperature consistency of the LED. However, the color rendering performance declines as a function of the CaCO3 growing amount, despite the presence of a KLS:Eu phosphor layer. Further works should be done to optimize the application of KLS:Eu in cooperation with scattering particles for a higher-quality LED device.
An autonomous robotic arm for efficient rock collection in uncharted territories
Authors: Deshmukh Sanjay , Thakker Bhaumik Hitesh , Gupte Vedangi Nilesh , Kapadia Taher Kutbuddin
Published: June 2024

The autonomous rock collector using robotic arm for exploration of unknown territories (ARCAxUT) is introduced as an innovative solution for the efficient retrieval of rock samples in unexplored space regions. Traditional, human-reliant methods are costly and hazardous, prompting the development of ARCAxUT. Equipped with a smart robotic arm, an RGB-D camera, and NUC computer, the system autonomously detects and estimates the mass of various rock samples. Validated in simulated and real-world environments, the algorithm ensures precise gripper control, achieving an impressive 95.4% accuracy in rock size estimation. This breakthrough offers transformative capabilities for space missions, revolutionizing celestial body sample collection and advancing broader societal implications in space exploration technologies.