bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Scale Analysis of Homologous Genes among Subgenomes of Bread Wheat (Triticum aestivum L.)
Authors: Zhou Caie , Dong Zhaonian , Zhang Ting , Wu Jianhui , Yu Shizhou , Zeng Qingdong , Han Dejun , Tong Wei
Published: April 2020

Determining the distribution and correspondence of genome-scale homologous genes in wheat are effective ways to uncover chromosome rearrangement that has occurred during crop evolution and domestication, which can contribute to improvements in crop breeding. High-resolution and comprehensive analysis of the wheat genome by the International Wheat Genome Sequencing Consortium (IWGSC) revealed a total of 88,733 high-confidence homologous genes of four major types (1:1:1, 1:1:0, 0:1:1 and 1:0:1) among the A, B and D subgenomes of wheat. This data was used to compare homologous gene densities among chromosomes, clarify their distribution and correspondence relationship, and compare their functional enrichment. The average density of 1:1:1 homologous genes was about 10 times more than the density of the other three types of homologous genes, although the homologous gene densities of the various chromosomes were similar within each homologous type. Three regions of exceptional density were detected in 1:1:1 homologous genes, the isolate peak on the tail of chromosome 4A, and the desert regions at the start of chromosome 7A and 7D. The correspondence between homologous genes of the wheat subgenomes demonstrated translocation between the tail segments of chromosome 4A and 5A, and the inversion of the segment of original 5A and 7B into the tail of 4A. The homologous genes on the inserting segments of 5A and 7B to 4A were highly enriched in nitrogen, primary metabolite and small molecular metabolism processes, compared with genes on other regions of the original 4A chromosome. This study provides a refined genome-scale reference of homologous genes for wheat molecular research and breeding, which will help to broaden the application of the wheat genome and can be used as a template for research on other polyploid plants.
CDKs in Sarcoma: Mediators of Disease and Emerging Therapeutic Targets
Authors: Kohlmeyer Jordan L , Gordon David J , Tanas Munir R , Monga Varun , Dodd Rebecca D , Quelle Dawn E
Published: April 2020

Sarcomas represent one of the most challenging tumor types to treat due to their diverse nature and our incomplete understanding of their underlying biology. Recent work suggests cyclin-dependent kinase (CDK) pathway activation is a powerful driver of sarcomagenesis. CDK proteins participate in numerous cellular processes required for normal cell function, but their dysregulation is a hallmark of many pathologies including cancer. The contributions and significance of aberrant CDK activity to sarcoma development, however, is only partly understood. Here, we describe what is known about CDK-related alterations in the most common subtypes of sarcoma and highlight areas that warrant further investigation. As disruptions in CDK pathways appear in most, if not all, subtypes of sarcoma, we discuss the history and value of pharmacologically targeting CDKs to combat these tumors. The goals of this review are to (1) assess the prevalence and importance of CDK pathway alterations in sarcomas, (2) highlight the gap in knowledge for certain CDKs in these tumors, and (3) provide insight into studies focused on CDK inhibition for sarcoma treatment. Overall, growing evidence demonstrates a crucial role for activated CDKs in sarcoma development and as important targets for sarcoma therapy.
Hox Gene Collinearity May Be Related to Noether Theory on Symmetry and Its Linked Conserved Quantity
Authors: Papageorgiou Spyros
Published: April 2020

Hox Gene Collinearity (HGC) is a fundamental property that controls the development of many animal species, including vertebrates. In the Hox gene clusters, the genes are located in a sequential order Hox1, Hox2, Hox3, etc., along the 3’ to 5’ direction of the cluster in the chromosome. During Hox cluster activation, the Hox genes are expressed sequentially in the ontogenetic units D1, D2, D3, etc., along the anterior–posterior axis (A-P) of the early embryo. This collinearity, first observed by E.B. Lewis, is surprising because the spatial collinearity of these structures (Hox clusters and embryos) correlates entities that differ by about four orders of magnitude. Biomolecular mechanisms alone cannot explain such correlations. Long-range physical interactions, such as diffusion or electric attractions, should be involved. A biophysical model (BM) was formulated, which, in alignment with the biomolecular processes, successfully describes the existing vertebrate genetic engineering data. One hundred years ago, Emmy Noether made a fundamental discovery in mathematics and physics. She proved, rigorously, that a physical system obeying a symmetry law (e.g., rotations or self-similarity) is followed by a conserved physical quantity. It is argued here that HGC obeys a ‘primitive’ self-similarity symmetry. In this case, the associated primitive conserved quantity is the irreversibly increasing ‘ratchet’-like Hoxgene ordering where some genes may be missing. The genes of a vertebrate Hox clusterare located along a finite straight line. The same order follows the ontogenetic unitsof the vertebrate embryo. Therefore, HGC is a manifestation of a primitive Noether Theory (NT). NT may be applied to other than the vertebrate case, for instance, to animals with a circular topological symmetry. For example, the observed abnormal Hox gene ordering of the echinoderm Hox clusters may be reproduced by a double-strand break of the circular Hox gene ordering and its subsequent incorporation in the flanking chromosome.
A DES-SST Based Assessment of Hydrodynamic Performances of the Wetted and Cavitating PPTC Propeller
Authors: Lungu Adrian
Published: April 2020

The paper describes an investigation of the hydrodynamic performances of a five-bladed controllable pitch propeller, whose geometry was provided by Schiffbau-Versuchsanstalt (SVA) Potsdam GmbH Model Basin. Both cavitating and non-cavitating regimes are numerically simulated for different advance ratio coefficients. The numerical approach is based on a finite volume approach in which closure to the turbulence is achieved through detached eddy simulation (DES). Propeller open water (POW) characteristics are computed, and the numerical solutions are validated through extensive comparisons with experimental data. In addition, the bi-phasic flow for the cavitating regime is simulated, for which comparisons with the cavitation sketches are performed to check the ability of the solver to estimate the cavitation extent. Grid convergence tests are performed for both working regimes together with validation and verification checks, not only to size the level of the numerical errors, but also to prove the robustness of the chosen numerical approach. Finally, a set of final remarks will conclude the present research.
Effects of China’s Collective Forestland Tenure Reform Policies on Forest Product Firm Values
Authors: Zhang Tingting , Yao Shunbo , Yu Jinna , Abu Hatab Assem , Liu Zhen
Published: April 2020

China’s collective forestland tenure reform has dramatically affected the business environment of domestic forest product firms. This study examines the impact of the said reform on the expected values of these firms, via the reaction of investors (as seen on the stock markets) towards the issuance of related policies. Based on signaling theory and the assumption that the Chinese stock markets are efficient in terms of work form, this study adopts an event study method and examines five policies during the 2003–2009 period. The numbers of forest product firms used in the examinations herein differ among the policies and range from 21 to 29. This study found that the policies have differentially affected the expected values of forest product firms and that the impact on firms lacking forestland holdings is generally more significant than that on firms that hold forestland. The findings of this study enhance our understanding of the effect of collective forestland tenure reform on the value of forest product firms; they also have implications on forest product firms as they work to adapt to the reform.
Enigmatic Histamine Receptor H4 for Potential Treatment of Multiple Inflammatory, Autoimmune, and Related Diseases
Authors: Mehta Pakhuri , Miszta Przemysław , Rzodkiewicz Przemysław , Michalak Olga , Krzeczyński Piotr , Filipek Sławomir
Published: April 2020

The histamine H4 receptor, belonging to the family of G-protein coupled receptors, is an increasingly attractive drug target. It plays an indispensable role in many cellular pathways, and numerous H4R ligands are being studied for the treatment of several inflammatory, allergic, and autoimmune disorders, including pulmonary fibrosis. Activation of H4R is involved in cytokine production and mediates mast cell activation and eosinophil chemotaxis. The importance of this receptor has also been shown in inflammatory models: peritonitis, respiratory tract inflammation, colitis, osteoarthritis, and rheumatoid arthritis. Recent studies suggest that H4R acts as a modulator in cancer, neuropathic pain, vestibular disorders, and type-2 diabetes, however, its role is still not fully understood.
A Method for Instant Estimation of the Temperature Experienced by Fire-Damaged Reinforced Concrete Structures Using Titanium
Authors: Sim Sang-Rak , Ryu Dong-Woo
Published: April 2020

When a concrete structure is exposed to fire, its structural safety is significantly compromised due to the spalling of members and scaling of concrete. In addition, its durability is substantially reduced due to certain chemical changes such as the dehydration of Ca(OH)2, the main hydration product of concrete, and the rehydration of CaO. Therefore, when fire damage occurs to a reinforced concrete (RC) building, rapid diagnosis and evaluation techniques are required for immediate repair and reinforcement, requiring a crucial step of quantitatively determining the heating temperature. This study aims to demonstrate a method of estimating the heating temperature experienced by fire damaged RC buildings. The experiments utilized two short RC column specimens with embedded titanium strips. The discoloration characteristics of titanium at high temperatures provided a quick, accurate, and simple mechanism for the estimation of the heating temperature by depth. Empirical equations were derived to estimate the heating temperature as a function of the discoloration characteristics of titanium. Thereafter, a comparison of this estimated temperature with the actual heating temperature measured using thermocouples revealed an average error of less than 20 °C, thereby demonstrating a significantly good correlation and an extremely high reliability of the proposed method.
In Situ Ternary Boride: Effects on Densification Process and Mechanical Properties of WC-Co Composite Coating
Authors: Bao Junfeng , Yu Yueguang , Liu Bowen , Jia Chengchang , Wu Chao
Published: April 2020

New coatings resistant to corrosion in high-temperature molten zinc aluminum were prepared by supersonic flame spraying of various composite powders. These composite powders were prepared by mixing, granulation, and heat treatment of various proportions of Mo–B4C powder and WC and Co powder. X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), high-angle annular dark-field scanning transmission electron microscopy (HAADF–STEM), energy dispersive X-ray spectroscopy (EDS), and mechanical analysis were used to study the effects of Mo–B4C on the microstructure, phase, porosity, bonding strength, and elastic modulus of the composite powder and coating. Results show that the addition of an appropriate quantity of Mo–B4C reacts with Co to form ternary borides CoMo2B2 and CoMoB. Ternary boride forms a perfect continuous interface, improving the mechanical properties and corrosion resistance property of the coating. When the amount of Mo–B4C added was 35.2%, the mechanical properties of the prepared coating reached optimal values: minimum porosity of 0.31 ± 0.15%, coating bonding strength of 77.81 ± 1.77 MPa, nanoindentation hardness of 20.12 ± 1.85 GPa, Young’s modulus of 281.52 ± 30.22 GPa, and fracture toughness of 6.38 ± 0.45 MPa·m1/2.
Vanadium-Oxide-Based Thin Films with Ultra-High Thermo-Optic Coefficients at 1550 nm and 2000 nm Wavelengths
Authors: Abdel-Rahman Mohamed , Bahidra Esam , Abas Ahmed Fauzi
Published: April 2020

In this paper, the temperature-dependent dielectric properties of vanadium-sesquioxide-based thin films are studied to assess their suitability for thermally tunable filters at optical communication wavelengths. Spectroscopic ellipsometry is utilized to measure the optical constants of vanadium oxide thin films at temperatures ranging from 25 °C to 65 °C. High thermo-optic coefficients (dn/dTs) were observed. The highest dn/dTs, measured at approximately 40 °C, were −8.4 × 10−3/°C and −1.05 × 10−2/°C at 1550 nm and 2000 nm, respectively.
On the Exponents of Exponential Dichotomies
Authors: Battelli Flaviano , Fečkan Michal
Published: April 2020

An exponential dichotomy is studied for linear differential equations. A constructive method is presented to derive a roughness result for perturbations giving exponents of the dichotomy as well as an estimate of the norm of the difference between the corresponding two dichotomy projections. This roughness result is crucial in developing a Melnikov bifurcation method for either discontinuous or implicit perturbed nonlinear differential equations.
Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity
Authors: Alhakamy Nabil A. , Fahmy Usama A. , Ahmed Osama A. A. , Caruso Giuseppe , Caraci Filippo , Asfour Hani Z. , Bakhrebah Muhammed A. , N. Alomary Mohammad , Abdulaal Wesam H. , Okbazghi Solomon Z. , Abdel-Naim Ashraf B. , Eid Basma G. , Aldawsari Hibah M. , Kurakula Mallesh , Mohamed Amir I.
Published: April 2020

This work aimed at improving the targeting and cytotoxicity of simvastatin (SMV) against colon cancer cells. SMV was encapsulated in chitosan polymers, followed by eudragit S100 microparticles. The release of SMV double coated microparticles was dependent on time and pH. At pH 7.4 maximum release was observed for 6 h. The efficiency of the double coat to target colonic tissues was confirmed using real-time X-ray radiography of iohexol dye. Entrapment efficiency and particle size were used in the characterization of the formula. Cytotoxicity of SMV microparticles against HCT-116 colon cancer cells was significantly improved as compared to raw SMV. Cell cycle analysis by flow cytomeric technique indicated enhanced accumulation of colon cancer cells in the G2/M phase. Additionally, a significantly higher cell fraction was observed in the pre-G phase, which highlighted enhancement of the proapoptotic activity of SMV prepared in the double coat formula. Assessment of annexin V staining was used for confirmation. Cell fraction in early, late and total cell death were significantly elevated. This was accompanied by a significant elevation of cellular caspase 3 activity. In conclusion, SMV-loaded chitosan coated with eudragit S100 formula exhibited improved colon targeting and enhanced cytotoxicity and proapoptotic activity against HCT-116 colon cancer cells.
Prevalence of Periodontal Disease among Obese Young Adult Population in Saudi Arabia—A Cross-Sectional Study
Authors: Thomas Julie Toby , Thomas Toby , Ahmed Masood , Kannan S Karthiga , Abdullah Zoha , Alghamdi Sara Ayid , Joseph Betsy
Published: April 2020

Background and objectives: We aimed to assess the prevalence of periodontal disease among obese young adults in Saudi Arabia and to analyze the association between different body mass indexes and the severity of periodontal disease. Materials and methods: This descriptive cross-sectional study consisted of 307 obese patients aged 18–39 years, with body mass index (BMI) ≥30. Demographic variables for periodontal disease, anthropometric parameters such as BMI along with clinical parameters such as oral hygiene index-simplified, community periodontal index (CPI) score and loss of attachment (LOA), were assessed. Multivariate binary logistic regression analysis was used to identify the predictors for chronic periodontitis in obese young adults between 18–40 years of age. Results: The majority of the participants (71.3%) had periodontal disease. Obese and extremely obese patients together showed a statistically significant difference in the age group of 21-30 years in terms of CPI score for inflammation (p < 0.05) and LOA (p < 0.001). Logistic regression analysis showed age (OR: 3.180; 95%CL: 1.337–7.561; p <.001), occasional dental visit (OR: 5.965; 95%CL: 3.130–11.368; p < 0.001), smoking >10 cigarettes (OR: 11.868; 95%CL: 3.588–39.254; p < 0.001) and poor oral hygiene status (OR: 17.250; 95%CL: 6.958–42.764; p < 0.001) were associated with a significantly higher risk of having periodontal disease. Conclusions: This study showed a high prevalence of periodontal disease in obese patients among the Saudi Arabian population.
Palatal Wound Healing with Primary Intention in a Rat Model—Histology and Immunohistomorphometry
Authors: Chaushu Liat , Rahmanov Gavrielov Marina , Chaushu Gavriel , Vered Marilena
Published: April 2020

Background and objectives: Subepithelial connective tissue graft (SCTG) from the palate has been considered as the “gold standard” for the treatment of deep gingival recessions. A single-incision technique was reported to allow primary wound healing. A palatal single incision was performed in a rat model. The present study assessed the histology and histomorphometry of palatal wound healing following surgical closure with primary intention. Materials and Methods: Twenty-six 6-month-old male Wistar rats weighing 427–650 g. An incision was made on the maxillary palate. A full thickness flap was raised palatally, and then repositioned and sutured. Two experimental groups: S—Study group, I—Intact control group. Half of the animals were sacrificed 7 days and the remaining 14 days postoperatively. Outcome parameters included—epithelial gap; inflammatory infiltration; vascular fraction, expression of myofibroblasts and stem cell markers within the oral epithelium and stromal cells and physical properties of stromal collagen fibers. Investigations were performed at two time-points (7 and 14 days) during the wound healing process. Results: The epithelial gap closed completely after 14 days. The inflammatory reaction and vascular fraction were relatively low. Surgical trauma downregulated the expression of cytokeratin (CK) 14 and CK 15, which returned to normal after 14 days. Epithelial differentiation was mediated through upregulation of connective tissue sex- determining-region-Y-box2 (SOX2). Epithelial SOX2, CD34, alpha smooth muscle actin (αSMA) and physical properties of stromal collagen fibers were not influenced by the surgical trauma. Conclusions: Surgical trauma followed by palatal wound healing with primary intention in a rat model heals within 14 days. It induces minimal inflammatory infiltration and vascular proliferation. Epithelization is exerted through promotion of epithelial differentiation from stem cells by connective tissue SOX2.
Pediatric Scoliosis Surgery—A Comprehensive Analysis of Treatment-Specific Variables and Trends in Latvia
Authors: Rüwald Julian M. , Upenieks Janis , Ositis Janis , Pycha Alexander , Avidan Yuval , Rüwald Anna L. , Eymael Robin L. , Schildberg Frank A.
Published: April 2020

Background and Objectives: There are currently no data available regarding pediatric scoliosis surgery in Latvia. The aim of this article is to present treatment specific variables, investigate their interrelation, and identify predictors for the length of stay after surgical pediatric scoliosis correction. Materials and Methods: This retrospective study included all surgical pediatric scoliosis corrections in Latvia for the years 2012 to 2016. Analyzed parameters were chosen to portray the patients’ demographics, pathology, as well as treatment specific variables. Descriptive, inferential, and linear regression statistics were calculated. Results: A total of 69 cases, 74% female and 26% male, were identified. The diagnostic subgroups consisted of 62% idiopathic (IDI) and 38% non-idiopathic (non-IDI) scoliosis cases. Non-IDI cases had significantly increased operation time, hospital stay, Cobb angle before surgery, and instrumented levels, while IDI cases showed significantly higher Cobb angle percentage correction. For all operated cases, the operation time and the hospital stay decreased significantly over the investigated time period. Early post-operative complications (PCs) occurred in 15.9% of the cases and were associated with increased hospital stay, instrumented levels, and Cobb angle before surgery. The linear regression analysis revealed that operation time and the presence of PCs were significant predictors for the length of the hospital stay. Conclusions: This is the first study to provide comprehensive insight into pediatric scoliosis surgery since its establishment in Latvia. Our regression model offers clinically applicable predictors and further underlines the significance of the operation length on the hospital stay. These results build the foundation for international comparison and facilitate improvement in the field.
Active Pulmonary Tuberculosis Triggered by Interferon Beta-1b Therapy of Multiple Sclerosis: Four Case Reports and a Literature Review
Authors: Sirbu Carmen Adella , Dantes Elena , Plesa Cristina Florentina , Docu Axelerad Any , Ghinescu Minerva Claudia
Published: April 2020

In this paper, we reported on four cases of severe pulmonary active tuberculosis in patients with multiple sclerosis (MS) undergoing interferon beta-1b (IFNβ-1b) therapy. Disease-modifying therapies (DMTs) in MS may increase the risk of developing active tuberculosis (TB) due to their impact on cellular immunity. Screening for latent infection with Mycobacterium tuberculosis (LTBI) should be performed, not only for the newer DMTs (alemtuzumab, ocrelizumab) but also for IFNβ-1b, alongside better supervision of these patients.
Timing of Endotracheal Intubation in Patients with Fulminant Enterovirus 71 Infection
Authors: Chen Shen-Dar , Ju Ying-Tzu , Wei Yu-Jen , Hsieh Min-Ling , Liu Ching-Chuan , Wu Jing-Ming , Wang Jieh-Neng
Published: April 2020

Background and objective: Enterovirus 71 (EV 71) infections may result in the rapid progression of cardiopulmonary failure. Early endotracheal intubation is considered to be of primary importance. However, the appropriate timing for this is still not known. The aim of this study is to investigate the timing of intubation of children with fulminant EV71 infection. Material and Methods: From March 1998 to May 2012, patients with severe EV71 infection who were admitted to the pediatric intensive care unit of the National Cheng Kung University Hospital were enrolled in this study. Medical records were retrospectively reviewed. The patients were classified into three groups in accordance with the outcome of intubation. We used rhombencephalitis grading to describe the neurological presentation of these patients. The study was approved by the institutional review board. Results: There were a total of 105 patients enrolled. Of these, 77 patients were in Grade I, and only three of them needed intubation, who were, however, soon extubated within 24 h. There were 10 patients in Grade II; nine of them needed intubation. In total, 18 patients belonged to Grade III, and all of them need to be intubated. We then compared the outcome of intubation of grades II and III. There was only one patient out of the nine patients in grade II who experienced failed extubation due to the progression of the disease. Among grade III patients, only four patients were successfully extubated. We also listed clinical parameters to determine which one could be a sign that indicated intubation. Comparing the favorable outcomes, cranial nerve involvement was a good indicator for the timing of intubation. Conclusions: This study showed that early intubation in Grade II provides favorable outcomes and improves morbidity and mortality. We also found that if cranial nerve involvement was present, then early intubation is indicated.
Cytomegalovirus Strain TB40/E Restrictions and Adaptations to Growth in ARPE-19 Epithelial Cells
Authors: Vo Mai , Aguiar Alexis , McVoy Michael A. , Hertel Laura
Published: April 2020

Despite displaying broad tropism in vivo, human cytomegalovirus (CMV) contained in bodily fluids replicates inefficiently in most cultured cell types except fibroblasts. As propagation in fibroblasts leads to the accumulation of genomic changes, a number of strains were generated by serial passaging on endothelial cells. One of these, TB40/E, was shown to contain a mixture of genetically distinct virus variants, and to retain tropism for fibroblasts, endothelial and epithelial cells. Cloning of an endotheliotropic subpopulation produced the TB40-BAC4 variant, extensively used in CMV tropism studies. Because TB40-BAC4 represents only one of the different variants comprising TB40/E, we generated a series of epithelial-cell adapted stocks derived from a TB40/E mixed stock, rather than from TB40-BAC4. Within two passages on ARPE-19 cells, virus populations were produced with the ability to enter and initiate replication with similar efficiencies in both epithelial cells and fibroblasts. Although the ability to release progeny also increased, cell-free virus yields from ARPE-19 cells remained consistently two to three-logs lower than from fibroblasts, hinting at the existence of a post-entry and post-genome synthesis block in epithelial cells. Multinucleated syncytia also rapidly appeared exclusively in ARPE-19 cell cultures, where their numbers and dimensions increased with virus passage. Irrespective of the number of infected nuclei comprising each syncytium, however, only one cytoplasmic virion assembly compartment was consistently observed, leading us to speculate that improvements in entry efficiency associated with ARPE-19 cell adaptation lead to the development of syncytia, which may negatively affect progeny release by limiting the amount of resources available to maturing virions.
Recent Advances in Strategies for Activation and Discovery/Characterization of Cryptic Biosynthetic Gene Clusters in Streptomyces
Authors: Nguyen Chung Thanh , Dhakal Dipesh , Pham Van Thuy Thi , Nguyen Hue Thi , Sohng Jae-Kyung
Published: April 2020

Streptomyces spp. are prolific sources of valuable natural products (NPs) that are of great interest in pharmaceutical industries such as antibiotics, anticancer chemotherapeutics, immunosuppressants, etc. Approximately two-thirds of all known antibiotics are produced by actinomycetes, most predominantly by Streptomyces. Nevertheless, in recent years, the chances of the discovery of novel and bioactive compounds from Streptomyces have significantly declined. The major hindrance for obtaining such bioactive compounds from Streptomyces is that most of the compounds are not produced in significant titers, or the biosynthetic gene clusters (BGCs) are cryptic. The rapid development of genome sequencing has provided access to a tremendous number of NP-BGCs embedded in the microbial genomes. In addition, the studies of metabolomics provide a portfolio of entire metabolites produced from the strain of interest. Therefore, through the integrated approaches of different-omics techniques, the connection between gene expression and metabolism can be established. Hence, in this review we summarized recent advancements in strategies for activating cryptic BGCs in Streptomyces by utilizing diverse state-of-the-art techniques.
Genetic Variation in Caenorhabditis elegans Responses to Pathogenic Microbiota
Authors: Huang Yuqing , Kammenga Jan E.
Published: April 2020

The bacterivorous nematode Caenorhabditis elegans is an important model species for understanding genetic variation of complex traits. So far, most studies involve axenic laboratory settings using Escherichia coli as the sole bacterial species. Over the past decade, however, investigations into the genetic variation of responses to pathogenic microbiota have increasingly received attention. Quantitative genetic analyses have revealed detailed insight into loci, genetic variants, and pathways in C. elegans underlying interactions with bacteria, microsporidia, and viruses. As various quantitative genetic platforms and resources like C. elegans Natural Diversity Resource (CeNDR) and Worm Quantitative Trait Loci (WormQTL) have been developed, we anticipate that expanding C. elegans research along the lines of genetic variation will be a treasure trove for opening up new insights into genetic pathways and gene functionality of microbiota interactions.
Kaempferide Enhances Chemosensitivity of Human Lung Adenocarcinoma A549 Cells Mediated by the Decrease in Phosphorylation of Akt and Claudin-2 Expression
Authors: Eguchi Hiroaki , Matsunaga Toshiyuki , Endo Satoshi , Ichihara Kenji , Ikari Akira
Published: April 2020

Claudins (CLDNs) play crucial roles in the formation of tight junctions. We have reported that abnormal expression of CLDN2 confers chemoresistance in the spheroids of human lung adenocarcinoma A549 cells. A food composition, which can reduce CLDN2 expression, may function to prevent the malignant progression. Here, we found that ethanol extract of Brazilian green propolis (EBGP) and kaempferide, a major component of EBGP, decrease CLDN2 expression. In the two-dimensional culture model, EBGP decreased the tight junctional localization of CLDN2 without affecting that of zonula occludens-1, an adaptor protein, and enhanced paracellular permeability to doxorubicin, a cytotoxic anticancer drug. EBGP reduced hypoxic stress, and enhanced the accumulation and sensitivity of doxorubicin in the spheroid of A549 cells. Kaempferide dose-dependently decreased CLDN2 expression, although dihydrokaempferide and pinocembrin did not. The phosphorylation of Akt, a regulatory factor of CLDN2 expression, was inhibited by kaempferide but not by dihydrokaempferide. The 2,3-double bond in the C ring may be important to inhibit Akt. Kaempferide decreased the mRNA level and promoter activity of CLDN2, indicating that it inhibits the transcription of CLDN2. In accordance with EBGP, kaempferide decreased the tight junctional localization of CLDN2 and increased a paracellular permeability to doxorubicin, suggesting that it diminished the paracellular barrier to small molecules. In addition, kaempferide reduced hypoxic stress, and enhanced the accumulation and sensitivity of doxorubicin in the spheroids. In contrast, dihydrokaempferide did not improve the sensitivity to doxorubicin. Further study is needed using an animal model, but we suggest that natural foods abundantly containing kaempferide are candidates for the prevention of the chemoresistance of lung adenocarcinoma.
Proinflammatory Dietary Intake is Associated with Increased Risk of Metabolic Syndrome and Its Components: Results from the Population-Based Prospective Study
Authors: Khan Imran , Kwon Minji , Shivappa Nitin , R. Hébert James , Kim Mi Kyung
Published: April 2020

Metabolic syndrome (MetS) is a major public health challenge throughout the world, although studies on its association with the inflammatory potential of diet are inconsistent. The aim of this prospective study was to assess the association between the Dietary Inflammatory Index (DII®) and the risk of MetS and its components in a Korean population. Data from 157,812 Korean adults (mean age 52.8 years; 53,304 men and 104,508 women with mean follow-up of 7.4 years) collected by members of the Korean Genome and Epidemiology Study form the basis for this report. DII scores were calculated based on Semi-Quantitative Food-Frequency Questionnaire data. Multivariable-adjusted Cox proportional hazard models were used to estimate the association between DII scores and MetS. In women, higher DII scores (pro-inflammatory diet) increased the risk of MetS (hazard ratio [HR]quintile5 v. 1 1.43; 95% confidence interval (CI) 1.21–1.69; p for trend ≤ 0.0001) and its five components. A positive association was observed for postmenopausal women, with a 50% higher risk of developing MetS (HRquintile5 v. 1 1.50; 95% CI 1.23–1.83; p for trend = 0.0008) after fully adjusting for potential confounders. Irrespective of the menopausal status of women, higher DII (=Q5) scores were positively associated with all 5 components of MetS (p < 0.05). In men, higher DII scores significantly increased the risk of low HDL cholesterol [HR]quintile5 v. 1 1.59 (1.27–1.99); p for trend = 0.0001], elevated waist circumferences [HR]quintile5 v. 1 1.28 (1.08–1.52); p for trend = 0.01], and high blood pressure [HR]quintile5 v. 1 1.17 (1.03–1.32); p for trend = 0.05]. These results indicate that diet with pro-inflammatory potential, as represented by higher DII scores, is prospectively associated with increased risk of MetS, and the relationship is stronger in women than in men.
Oral Microbiota: Discovering and Facing the New Associations with Systemic Diseases
Authors: Patini Romeo
Published: April 2020

The economic crisis of the first decades of the 2000s had serious repercussions on the economy of individual countries, producing a gradual impoverishment of populations. The reduction in financial resources has significantly reduced citizens’ access to care, forcing them to abandon preventive medicine treatments and check-ups. The health of the oral cavity, which had long been considered of secondary importance when compared with systemic pathologies whose course can be potentially fatal for the patient, has therefore been strongly neglected. In recent years, however, new mechanisms of etiology of systemic diseases have been studied with the aim of evaluating some aspects still unknown. The microbiota, whose interest has grown considerably in the national scientific community, was immediately considered as a key factor in the pathogenesis of some disorders. These analyses have also benefited from numerous advances in the field of crop and molecular diagnostics in the microbiological field. Although pioneering studies have focused on the microbiota of the gastro-intestinal system, subsequent evidence has also been drawn from various studies conducted on the oral microbiota. What emerged is that oral microbiota dysbiosis has been associated with numerous systemic diseases. Therefore, the purpose of this Special Issue is to encourage scientific research on the topic of the relationship between the oral microbiota and systemic diseases, also inviting the use of new techniques for culture and molecular diagnosis. Particular attention will be given to original works in vivo and to literature reviews provided they are carried out with a systematic approach and, if possible, supported by additional quantitative analyses.
Venereal Transmission of Vesicular Stomatitis Virus by Culicoides sonorensis Midges
Authors: Rozo-Lopez Paula , Londono-Renteria Berlin , Drolet Barbara S.
Published: April 2020

Culicoides sonorensis biting midges are well-known agricultural pests and transmission vectors of arboviruses such as vesicular stomatitis virus (VSV). The epidemiology of VSV is complex and encompasses a broad range of vertebrate hosts, multiple routes of transmission, and diverse vector species. In temperate regions, viruses can overwinter in the absence of infected animals through unknown mechanisms, to reoccur the next year. Non-conventional routes for VSV vector transmission may help explain viral maintenance in midge populations during inter-epidemic periods and times of adverse conditions for bite transmission. In this study, we examined whether VSV could be transmitted venereally between male and female midges. Our results showed that VSV-infected females could venereally transmit virus to uninfected naïve males at a rate as high as 76.3% (RT-qPCR), 31.6% (virus isolation) during the third gonotrophic cycle. Additionally, VSV-infected males could venereally transmit virus to uninfected naïve females at a rate as high as 76.6% (RT-qPCR), 49.2% (virus isolation). Immunofluorescent staining of micro-dissected reproductive organs, immunochemical staining of midge histological sections, examination of internal reproductive organ morphology, and observations of mating behaviors were used to determine relevant anatomical sites for virus location and to hypothesize the potential mechanism for VSV transmission in C. sonorensis midges through copulation.
Enhancing Precipitation Estimates Through the Fusion of Weather Radar, Satellite Retrievals, and Surface Parameters
Authors: Wehbe Youssef , Temimi Marouane , Adler Robert F.
Published: April 2020

Accurate and timely monitoring of precipitation remains a challenge, particularly in hyper-arid regions such as the United Arab Emirates (UAE). The aim of this study is to improve the accuracy of the Integrated Multi-satellitE Retrievals for the Global Precipitation Measurement (GPM) mission’s latest product release (IMERG V06B) locally over the UAE. Two distinct approaches, namely, geographically weighted regression (GWR), and artificial neural networks (ANNs) are tested. Daily soil moisture retrievals from the Soil Moisture Active Passive (SMAP) mission (9 km), terrain elevations from the Advanced Spaceborne Thermal Emission and Reflection digital elevation model (ASTER DEM, 30 m) and precipitation estimates (0.5 km) from a weather radar network are incorporated as explanatory variables in the proposed GWR and ANN model frameworks. First, the performances of the daily GPM and weather radar estimates are assessed using a network of 65 rain gauges from 1 January 2015 to 31 December 2018. Next, the GWR and ANN models are developed with 52 gauges used for training and 13 gauges reserved for model testing and seasonal inter-comparisons. GPM estimates record higher Pearson correlation coefficients (PCC) at rain gauges with increasing elevation (z) and higher rainfall amounts (PCC = 0.29 z0.12), while weather radar estimates perform better for lower elevations and light rain conditions (PCC = 0.81 z−0.18). Taylor diagrams indicate that both the GWR- and the ANN-adjusted precipitation products outperform the original GPM and radar estimates, with the poorest correction obtained by GWR during the summer period. The incorporation of soil moisture resulted in improved corrections by the ANN model compared to the GWR, with relative increases in Nash–Sutcliffe efficiency (NSE) coefficients of 56% (and 25%) for GPM estimates, and 34% (and 53%) for radar estimates during summer (and winter) periods. The ANN-derived precipitation estimates can be used to force hydrological models over ungauged areas across the UAE. The methodology is expandable to other arid and hyper-arid regions requiring improved precipitation monitoring.
Coastal Wind Measurements Using a Single Scanning LiDAR
Authors: Shimada Susumu , Goit Jay Prakash , Ohsawa Teruo , Kogaki Tetsuya , Nakamura Satoshi
Published: April 2020

A wind measurement campaign using a single scanning light detection and ranging (LiDAR) device was conducted at the Hazaki Oceanographical Research Station (HORS) on the Hazaki coast of Japan to evaluate the performance of the device for coastal wind measurements. The scanning LiDAR was deployed on the landward end of the HORS pier. We compared the wind speed and direction data recorded by the scanning LiDAR to the observations obtained from a vertical profiling LiDAR installed at the opposite end of the pier, 400 m from the scanning LiDAR. The best practice for offshore wind measurements using a single scanning LiDAR was evaluated by comparing results from a total of nine experiments using several different scanning settings. A two-parameter velocity volume processing (VVP) method was employed to retrieve the horizontal wind speed and direction from the radial wind speed. Our experiment showed that, at the current offshore site with a negligibly small vertical wind speed component, the accuracy of the scanning LiDAR wind speeds and directions was sensitive to the azimuth angle setting, but not to the elevation angle setting. In addition to the validations for the 10-minute mean wind speeds and directions, the application of LiDARs for the measurement of the turbulence intensity (TI) was also discussed by comparing the results with observations obtained from a sonic anemometer, mounted at the seaward end of the HORS pier, 400 m from the scanning LiDAR. The standard deviation obtained from the scanning LiDAR measurement showed a greater fluctuation than that obtained from the sonic anemometer measurement. However, the difference between the scanning LiDAR and sonic measurements appeared to be within an acceptable range for the wind turbine design. We discuss the variations in data availability and accuracy based on an analysis of the carrier-to-noise ratio (CNR) distribution and the goodness of fit for curve fitting via the VVP method.
Covert Timing Channel Analysis Either as Cyber Attacks or Confidential Applications
Authors: Al-Eidi Shorouq , Darwish Omar , Chen Yuanzhu
Published: April 2020

Covert timing channels are an important alternative for transmitting information in the world of the Internet of Things (IoT). In covert timing channels data are encoded in inter-arrival times between consecutive packets based on modifying the transmission time of legitimate traffic. Typically, the modification of time takes place by delaying the transmitted packets on the sender side. A key aspect in covert timing channels is to find the threshold of packet delay that can accurately distinguish covert traffic from legitimate traffic. Based on that we can assess the level of dangerous of security threats or the quality of transferred sensitive information secretly. In this paper, we study the inter-arrival time behavior of covert timing channels in two different network configurations based on statistical metrics, in addition we investigate the packet delaying threshold value. Our experiments show that the threshold is approximately equal to or greater than double the mean of legitimate inter-arrival times. In this case covert timing channels become detectable as strong anomalies.
Fast Screening of Whole Blood and Tumor Tissue for Bladder Cancer Biomarkers Using Stochastic Needle Sensors
Authors: Stefan-van Staden Raluca-Ioana , Gheorghe Damaris-Cristina , Jinga Viorel , Sima Cristian Sorin , Geanta Marius
Published: April 2020

Bladder cancer is one of the most common urologic malignancies, which is more frequent in men than in women. The early diagnosis for this type of cancer still remains a challenge, therefore, the development of a fast screening test for whole blood and tumor tissue samples may save lives. Four biomarkers, p53, E-cadherin, bladder tumor antigen (BTA), and hyaluronic acid were considered for the screening tests using stochastic needle sensors. Three stochastic needle sensors, based on graphite powder and modified with three types of chitosan, were designed and characterized for the screening test. The proposed sensors showed low limits of quantification, and high sensitivity and selectivity levels. The recoveries of p53, E-cadherin, BTA, and hyaluronic acid in whole blood samples and tissue samples were higher than 95.00% with a relative standard deviation lower than 1.00%.
Perception of a Haptic Stimulus Presented Under the Foot Under Workload
Authors: Chapwouo Tchakoute Landry Delphin , Menelas Bob-Antoine J.
Published: April 2020

It is clear that the haptic channel can be exploited as a communication medium for several tasks of everyday life. Here we investigated whether such communication can be altered in a cognitive load situation. We studied the perception of a vibrotactile stimulus presented under the foot when the attention is loaded by another task (cognitive load). The results demonstrated a significant influence of workload on the perception of the vibrotactile stimulus. Overall, we observed that the average score in the single-task (at rest) condition was greater than the overall mean score in the dual-task conditions (counting forwards, counting backwards, and walking). The walking task was the task that most influenced the perception of the vibrotactile stimulus presented under the foot.
Adversarial Networks for Scale Feature-Attention Spectral Image Reconstruction from a Single RGB
Authors: Liu Pengfei , Zhao Huaici
Published: April 2020

Hyperspectral images reconstruction focuses on recovering the spectral information from a single RGBimage. In this paper, we propose two advanced Generative Adversarial Networks (GAN) for the heavily underconstrained inverse problem. We first propose scale attention pyramid UNet (SAPUNet), which uses U-Net with dilated convolution to extract features. We establish the feature pyramid inside the network and use the attention mechanism for feature selection. The superior performance of this model is due to the modern architecture and capturing of spatial semantics. To provide a more accurate solution, we propose another distinct architecture, named W-Net, that builds one more branch compared to U-Net to conduct boundary supervision. SAPUNet and scale attention pyramid WNet (SAPWNet) provide improvements on the Interdisciplinary Computational Vision Lab at Ben Gurion University (ICVL) datasetby 42% and 46.6%, and 45% and 50% in terms of root mean square error (RMSE) and relative RMSE, respectively. The experimental results demonstrate that our proposed models are more accurate than the state-of-the-art hyperspectral recovery methods
Implementation of System Operation Modes for Health Management and Failure Prognosis in Cyber-Physical Systems
Authors: Ruiz-Arenas Santiago , Rusák Zoltán , Mejía-Gutiérrez Ricardo , Horváth Imre
Published: April 2020

Cyber-physical systems (CPSs) have sophisticated control mechanisms that help achieve optimal system operations and services. These mechanisms, imply considering multiple signal inputs in parallel, to timely respond to varying working conditions. Despite the advantages that control mechanisms convey, they bring new challenges in terms of failure prevention. The compensatory action the control exerts cause a fault masking effect, hampering fault diagnosis. Likewise, the multiple information inputs CPSs have to process can affect the timely system response to faults. This article proposes a failure prognosis method, which combines time series-based forecasting methods with statistically based classification techniques in order to investigate system degradation and failure forming on system levels. This method utilizes a new approach based on the concept of the system operation mode (SOM) that offers a novel perspective for health management that allows monitoring the system behavior, through the frequency and duration of SOMs. Validation of this method was conducted by systematically injecting faults in a cyber-physical greenhouse testbed. The obtained results demonstrate that the degradation and fault forming process can be monitored by analyzing the changes of the frequency and duration of SOMs. These indicators made possible to estimate the time to failure caused by various failures in the conducted experiments.