bluuebunny/crossref_metadata_2025 huggingface dataset:
When Does Da Vanci Robotic Surgical Systems Come Into Play?
Authors: Kao Hao-Yun , Yang Yi-Chen , Hung Yu-Han , Wu Yenchun Jim
Published: January 2022

The emergent of medical science and technology has risen the minimally invasive surgery. Da Vinci Robotic Surgical Systems (RSS) is the trend at present. Compared with the past surgical methods, many studies related to RSS tend to explore postoperative outcomes and quality of life or compare the advantages and disadvantages than the other surgery. Few studies to understand the patients' willing who use RSS. This study mainly explores the patients' willingness and adopts the Technology Acceptance Model (TAM) as the theoretical foundation, and appended the trust concept to discuss. The study was a retrospective study and used a structured questionnaire to conduct a survey. The subjects included the patients with single-disease who had used RSS in a Medical Center of Southern Taiwan but excluded the patients with multiple disorder. This study conducted SPSS 22.0 and Smart PLS 2.0 software for statistical analysis, which included descriptive statistical analysis and applied Partial Least Squares (PLS) analysis to test the research model and to examine the established hypotheses. A total of 136 cases were collected in this study. Study validation was tested. Trust positively affects Perceived Usefulness (β = 0.550) and Perceived Ease of Use (β = 0.300). Perceived Ease of Use positively affects Perceived Usefulness (β = 0.188). Perceived Usefulness positively affects Attitude Toward Using (β = 0.589. Attitude Toward Using positively affects Behavioral Intention (β = 0.446. The relationship between perceived Ease of Use and Attitude toward Using was insignificant. Additionally, the relationship between Perceived Usefulness and Behavioral Intention was insignificant. In the research results, we found that patients are mostly in the middle and high age groups, and if the patient himself feels that RSS is extremely helpful to his illness, the intensity of his choice of intention will be high. In comparison, the information related to RSS has been clearly known, it does not directly affect the selection intention. According to age, most of the choices of RSS is based on safety and risk considerations, and it is beneficial to the patient himself, but RSS is also more expensive. We recommended that the government consider ββ reimbursing the RSS process in health insurance programs to meet the needs and expectations of patients.
Territorialized Commons and Social Movements: Legal Appropriations of Collective Spaces in Berlin and Santiago de Chile
Authors: Amacher David , Kip Markus , Opazo Daniel
Published: January 2022

This article aims to explore the relationship between territorialized commons and movements and how the use of legal strategies of appropriation mediates this relationship. We study two recent commoning practices for collective space in Berlin and Santiago de Chile that have appropriated publicly owned land for their initiative, engaging with legal institutions to meet the ends of the movement. This type of relationship to movements has not been given serious consideration in the commons literature and we ask how these commoning initiatives make the appropriated land a common resource for the movement and resist privatization. Based on secondary sources and interviews with activists, we conclude that both initiatives in Berlin and Santiago de Chile have drawn on legal frameworks for transformative purposes while also politicizing the question of public land as an asset for civic and commoning use. However, said frameworks do not replace activist engagement and self-organization as the main element behind commoning processes.
Farming Households' Satisfaction With Quality of Agricultural Extension Services: A Case Study of Quang Binh Province, Vietnam
Authors: Truong Dinh Duc
Published: January 2022

Agriculture is an important sector of Vietnam, in which agricultural extension (AE) is a long-standing activity but was officially institutionalized in 1993 when Vietnam reformed its economic model. The AE system in Vietnam is organized quite closely from the central to local levels with various forms of AE. This study assesses the satisfaction of farmers with AE services in Quang Binh province, Vietnam. The results of a survey of 455 farmers show a positive relationship between quality and satisfaction. Factors such as assurance, reliability, and sympathy are important factors in AE service quality. The study also provides recommendations to strengthen AE services, including updating technical information, organizing demonstration models, and stronger investment in AE systems at all levels.
Heavy Metal Stress Alleviation Through Omics Analysis of Soil and Plant Microbiome
Authors: Phurailatpam Laccy , Dalal Vijay Kumar , Singh Namrata , Mishra Sushma
Published: January 2022

Heavy metal (HM) contamination of soil and water resources is a global concern, which not only limits crop yield and quality, but also has serious environmental effects. Due to the non-biodegradable nature and toxicity, high concentration of HMs in food and environment is a serious threat to the entire ecosystem. Moreover, the target of supplying safe and quality food to the rising human population (expected to reach ~9–10 bn by the year 2050), necessitates effective treatment of the HM-contaminated soil. Various microbe-mediated bioremediation strategies such as biosorption, bioprecipiation, biostimulation, etc., have been found to be effective in uptake and conversion of HMs to less toxic forms. Further, in the past few years, the use of soil and plant-associated microbiome for HM stress alleviation is gaining attention among the scientific community. In general, microbes are spectacular in being dynamic and more responsive to environmental conditions in comparison to their host plants. Moreover, with the advancements in high throughput sequencing technologies, the focus is eventually shifting from just structural characterization to functional insights into the microbiome. The microbes inhabiting the HM-contaminated environments or associated with HM-tolerant plants are a source for exploring HM-tolerant microbial communities, which could be used for enhancing bioremediation efficiency and conferring HM tolerance in plants. This review discusses the application of omics techniques including metagenomics, metatranscriptomics, metaproteomics, and metabolomics, for rapid and robust identification of HM-tolerant microbial communities, mining novel HM resistance genes, and fabricating the HM resistome.
Kinematic Analysis During Straight Line Free Swimming in Horses: Part 2 - Hindlimbs
Authors: Santosuosso Emma , Leguillette Renaud , Vinardell Tatiana , Filho Silvio , Massie Shannon , McCrae Persephone , Johnson Sarah , Rolian Campbell , David Florent
Published: January 2022

Background

Swimming is used for rehabilitation and conditioning purposes in equine sports medicine. We described the swimming kinematics of the equine forelimbs in Part 1. The aim of Part 2 is to assess stifle, tarsus, and hind fetlock joints kinematics in swimming horses. The objectives were 1- to calculate and compare joint angles during swimming against passive mobilizations (PM), 2- to determine joints angular velocities during a swimming stride cycle.

Methods

Eleven elite endurance horses were used to swim in a 100-meter straight pool. Underwater (swimming) and overground PM videos were recorded from the horses' left side. Joint markers were applied on the lateral hoof wall, lateral metatarsal epicondyle, lateral aspect of the talus, lateral femoral epicondyle, and great trochanter of the femur. As a reference, maximal fetlock, tarsus, and stifle flexion/extension angles were determined during PM overground. Differences between angle extrema, angular velocities, and range of motion (ROM) were statistically compared.

Results

The tarsus ROM was similar during PM and swimming. The stifle and fetlock ROM were greater during PM, although the stifle flexion was greater during swimming. The stifle and tarsus had the greatest hindlimb angular velocity during the swimming cycle. Greater angular velocities were observed during the retraction phase for all the hindlimb joints.

Conclusion

A short retraction phase with great angular velocity for the joints of interest characterized the swimming pattern observed. Swimming may be beneficial in horses when an increased ROM of the tarsus and stifle or a reduced fetlock extension is indicated for rehabilitation purposes.
Using Extract From the Stems and Leaves of Yizhi (Alpiniae oxyphyllae) as Feed Additive Increases Meat Quality and Intestinal Health in Ducks
Authors: Ji Fengjie , Gu Lihong , Rong Guang , Hu Chengjun , Sun Weiping , Wang Dingfa , Peng Weiqi , Lin Dajie , Liu Quanwei , Wu Hongzhi , Dai Haofu , Zhou Hanlin , Xu Tieshan
Published: January 2022

Yizhi (Alpiniae Oxyphyllae, A. oxyphylla) has been widely used as an important traditional Chinese medicinal herb for centuries. Existing studies have shown that A. oxyphylla has numerous benefits in human and animal health. We hypothesized that extract from the stems and leaves of A. oxyphylla (AOE) as a feed additive may have positive effects on animal health and products. Thus, this study was conducted to evaluate the effects of AOE as a feed additive on growth performance, serum biochemical parameters, intestinal morphology, microbial composition, and meat quality in Jiaji ducks. A total of 240 Jiaji ducks of 42 days old (1675.8 ± 44.2 g, male: female ratio = 1:1) were blocked based on body weight and randomly allocated into four dietary treatments with three replicates that each had 20 duck individuals. The dietary treatments included: basal diet, control group (CK); basal diet supplementation with 30 mg/kg (Y1), 80 mg/kg (Y2), and 130 mg/kg (Y3) AOE, respectively, and lasted for 49 days. The results showed that average daily feed intake from day 42 to day 60 was decreased with the increasing level of AOE (P < 0.05). Compared with the CK group, the groups with AOE supplementation decreased serum LDL-C level (P < 0.05), the addition of 30 mg/kg AOE increased total amino acids, essential amino acids, branched-chain amino acids, nonessential amino acids, and umami taste amino acids (P < 0.05), but decreased selenium and zinc concentrations in breast muscle (P < 0.05). In addition, the supplementation of 30 or 130 mg/kg AOE significantly increased jejunal villus height (P < 0.05) and tended to increase the ratio of villus height to crypt depth in the jejunum (P = 0.092) compared to the CK group. Moreover, the addition of 30 mg/kg AOE showed a higher abundance of genus unclassified Bacteroidales and genus unclassified Ruminococcaceae than the CK group (P < 0.05). Therefore, dietary supplementation with 30 mg/kg AOE increased meat nutrition profile and flavor through promoting amino acid contents in breast muscle, as well as maintained intestine integrity and modulated the microbial composition. In conclusion, AOE as an antibiotic alternative displayed potential in maintaining intestinal health and improving meat quality.
Strategies to Reduce Crop Water Footprint in Intensive Wheat-Maize Rotations in North China Plain
Authors: Zhang Di , Li Dongxiao , Li Haoran , Wang Hongguang , Liu Jinna , Ju Hui , Batchelor William D. , Li Ruiqi , Li Yanming
Published: January 2022

The intensive use of groundwater and nitrogen fertilizer has led to serious negative impacts on the environment of the North China Plain (NCP). Water footprint is an emerging approach to assess the consumptive water use and the environmental impacts on winter wheat-summer maize systems. A seven-year rotation experiment was conducted to collect data on wheat and maize growth response to nitrogen rates and irrigation schedules and to explore how the water footprint was affected, using DSSAT 4.6 Wheat and Maize crop models. Results showed that the increase in farm inputs contributed greatly to the increase in water footprint, primarily through the grey water footprint. The water footprints of maize and wheat were more sensitive to the nitrogen rate and irrigation, respectively. An irrigation of 160 mm produced a minimum total water footprint and higher yield for wheat. The grey water footprint of maize increased linearly when the nitrogen rate applied to maize exceeded 60 kg ha−1. Water-saving irrigation with 300 kg ha−1 of nitrogen can maintain a higher grain yield and have little impact on the environment. The approach used and the results can help to develop management strategies to maintain high yields while minimizing the water footprint in NCP.
Comparison of the Intestinal Structure and Intestinal Microbiome between Two Geographically Isolated Populations of Culter alburnus
Authors: Wang Jun , Xu Bowen , Zhang Zhiyi , Zhou Lu , Zhang Guoqi , Zhang Youliang , Wang Chenghui
Published: January 2022

Geographical populations of Culter alburnus inhabiting different regions of China present substantial differences in their reproduction and development characters. This study compared the intestinal structures, digestive enzyme activities, and intestinal microbiomes in the Xingkai (XK) Lake and the Dianshan (DS) Lake populations of C. alburnus collected in two isolated and contrasting river systems. We wanted to discover whether the intestinal structure and functional divergence were formed in the two populations due to adaptive evolution caused by geographical isolation. Our study indicated that higher intestinal villi, thicker intestinal mucosa layer and intestinal muscle layer, and significantly higher activity of α-amylase were identified in the XK population. Moreover, quite different intestinal microbiomes were presented in the two populations, with the higher abundance of Bacteroidetes and Firmicutes in the XK population. The significantly different intestinal microbiome in the XK population was functionally enriched in carbohydrate, lipid, and amino acid metabolism by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Our findings indicated that substantial adaptative divergence in the intestinal structures and intestine microbiomes was formed in the two populations due to long-term geographical isolation, which may have strongly affected the digestion and absorption ability of the XK population compared with the DS population.
Assessment of ERA-5 Temperature Variability in the Middle Atmosphere Using Rayleigh LiDAR Measurements between 2005 and 2020
Authors: Mariaccia Alexis , Keckhut Philippe , Hauchecorne Alain , Claud Chantal , Le Pichon Alexis , Meftah Mustapha , Khaykin Sergey
Published: January 2022

In this study, the temperature biases and the ability of the ERA-5 product to reproduce the LiDAR variability in the 30–80 km altitude range were evaluated for the period 2005–2020, both for the winter and the summer months. During winter, temperatures from the ERA-5 dataset were in good agreement with LiDAR observations up to 45 km, while in the mesosphere, almost 70% of the ERA-5 profiles were cooler than those from LiDAR, except around 65 km. During summer, negative biases of −3 K were observed up to the stratopause, while significant positive biases of more than +10 K were found in the mesosphere. For the winter months, the variability observed by LiDAR, even during sudden stratospheric warming (SSWs) events, was reproduced accurately by the model in the upper stratosphere, but not in the mesosphere. Surprisingly, the LiDAR variability mainly due to propagating gravity waves in the summertime was also not reproduced by ERA-5 in the whole middle atmosphere. The model uncertainty associated with this variability, evaluated afterward with a new method, grew as expected with altitude and was more significant in winter than summer. A principal component analysis of the fluctuations of the temperature differences between the LiDAR and ERA-5 was performed to investigate the vertical coupling between 30 km and 70 km. The three first vertical modes illustrated 76% and 78% of the fluctuations of the temperature difference profiles in summer and winter, respectively, confirming the connection between the studied layers. The leading modes of the summer (49%) and winter (42%) possessed an anti-correlation between the upper stratosphere and the mesosphere, where fluctuations increased (at least ±5 K at 65 km) for both seasons due to the coarse vertical resolution in the model. The other modes showed an agreement between the LiDAR and ERA-5 fluctuations in the upper stratosphere and had a wave-like structure mainly located in the mesosphere, confirming that the model either overlooked or simulated imprecisely the gravity waves, leading to mesospheric inversions. Finally, SSWs impacted the ERA-5 temperature (deviation of ±3 K) some days before and after its trigger around the stratopause.
South America Seasonal Precipitation Prediction by Gradient-Boosting Machine-Learning Approach
Authors: Monego Vinicius Schmidt , Anochi Juliana Aparecida , de Campos Velho Haroldo Fraga
Published: January 2022

Machine learning has experienced great success in many applications. Precipitation is a hard meteorological variable to predict, but it has a strong impact on society. Here, a machine-learning technique—a formulation of gradient-boosted trees—is applied to climate seasonal precipitation prediction over South America. The Optuna framework, based on Bayesian optimization, was employed to determine the optimal hyperparameters for the gradient-boosting scheme. A comparison between seasonal precipitation forecasting among the numerical atmospheric models used by the National Institute for Space Research (INPE, Brazil) as an operational procedure for weather/climate forecasting, gradient boosting, and deep-learning techniques is made regarding observation, with some showing better performance for the boosting scheme.
Laser-Engraved Liquid Metal Circuit for Wearable Electronics
Authors: Liang Shuting , Chen Xingyan , Li Fengjiao , Song Na
Published: January 2022

Conventional patterning methods for producing liquid metal (LM) electronic circuits, such as the template method, use chemical etching, which requires long cycle times, high costs, and multiple-step operations. In this study, a novel and reliable laser engraving micro-fabrication technology was introduced, which was used to fabricate personalized patterns of LM electronic circuits. First, by digitizing the pattern, a laser printing technology was used to burn a polyethylene (PE) film, where a polydimethylsiloxane (PDMS) or paper substrate was used to produce grooves. Then, the grooves were filled with LM and the PE film was removed; finally, the metal was packaged with PDMS film. The experimental results showed that the prepared LM could fabricate precise patterned electronic circuits, such as golden serpentine curves and Peano curves. The minimum width and height of the LM circuit were 253 μm and 200 μm, respectively, whereas the printed LM circuit on paper reached a minimum height of 26 μm. This LM flexible circuit could also be adapted to various sensor devices and was successfully applied to heart rate detection. Laser engraving micro-processing technologies could be used to customize various high-resolution LM circuit patterns in a short time, and have broad prospects in the manufacture of flexible electronic equipment.
Management of Oligoprogression in Patients with Metastatic NSCLC Harboring ALK Rearrangements
Authors: Pisano Chiara , De Filippis Marco , Jacobs Francesca , Novello Silvia , Reale Maria Lucia
Published: January 2022

Personalized treatment based on driver molecular alterations, such as ALK rearrangement, has revolutionized the therapeutic management of advanced oncogene-addicted NSCLC patients. Multiple effective ALK tyrosine kinase inhibitors (TKIs), with the amelioration of the activity at central nervous system level, are now available, leading to substantial prognosis improvement. The exposure to TKIs triggers resistance mechanisms and the sequential administration of other TKIs and chemotherapy is, for the most part, not targeted. In this context, extending the benefit deriving from precision medicine is paramount, above all, when disease progression occurs in a limited number of sites. Retrospective data indicate that, in oligoprogressive disease, targeted therapy beyond progression combined with definitive local treatment of the progressing site(s) is an effective alternative. In these cases, a multidisciplinary approach becomes essential for an integrated treatment strategy, depending on the site of disease progression, in order to improve not only survival, but also quality of life. In this review we provide an updated and comprehensive overview of the main treatment strategies in cases of ALK rearranged oligoprogression, including systemic treatment as well as local therapy, and report a real-world clinical story, with the final aim of identifying the most promising management for this subset of patients.
The Role of Sarcopenia and Myosteatosis in Short- and Long-Term Outcomes Following Curative-Intent Surgery for Hepatocellular Carcinoma in a European Cohort
Authors: Meister Franziska Alexandra , Lurje Georg , Verhoeven Suekran , Wiltberger Georg , Heij Lara , Liu Wen-Jia , Jiang Decan , Bruners Philipp , Lang Sven Arke , Ulmer Tom Florian , Neumann Ulf Peter , Bednarsch Jan , Czigany Zoltan
Published: January 2022

Alterations of body composition, especially decreased muscle mass (sarcopenia) and impaired muscle quality (myosteatosis), are associated with inferior outcomes in various clinical conditions. The data of 100 consecutive patients who underwent partial hepatectomy for hepatocellular carcinoma (HCC) at a German university medical centre were retrospectively analysed (May 2008–December 2019). Myosteatosis and sarcopenia were evaluated using preoperative computed-tomography-based segmentation. We investigated the predictive role of alterations in body composition on perioperative morbidity, mortality and long-term oncological outcome. Myosteatotic patients were significantly inferior in terms of major postoperative complications (Clavien–Dindo ≥ 3b; 25% vs. 5%, p = 0.007), and myosteatosis could be confirmed as an independent risk factor for perioperative morbidity in multivariate analysis (odds ratio: 6.184, confidence interval: 1.184–32.305, p = 0.031). Both sarcopenic and myosteatotic patients received more intraoperative blood transfusions (1.6 ± 22 vs. 0.3 ± 1 units, p = 0.000; 1.4 ± 2.1 vs. 0.3 ± 0.8 units, respectively, p = 0.002). In terms of long-term overall and recurrence-free survival, no statistically significant differences could be found between the groups, although survival was tendentially worse in patients with reduced muscle density (median survival: 41 vs. 60 months, p = 0.223). This study confirms the prognostic role of myosteatosis in patients suffering from HCC with a particularly strong value in the perioperative phase and supports the role of muscle quality over quantity in this setting. Further studies are warranted to validate these findings.
Primary Effusion Lymphoma: A Clinicopathologic Perspective
Authors: Gathers Diamone , Galloway Emily , Kelemen Katalin , Rosenthal Allison , Gibson Sarah , Munoz Javier
Published: January 2022

Primary effusion lymphoma (PEL) is a rare, aggressive B-cell lymphoma that usually localizes to serous body cavities to subsequently form effusions in the absence of a discrete mass. Although some tumors can develop in extracavitary locations, the areas most often affected include the peritoneum, pleural space, and the pericardium. PEL is associated with the presence of human herpesvirus 8 (HHV8), also called the Kaposi sarcoma-associated herpesvirus (KSHV), with some variability in transformation potential suggested by frequent coinfection with the Epstein-Barr virus (EBV) (~80%), although the nature of the oncogenesis is unclear. Most patients suffering with this disease are to some degree immunocompromised (e.g., Human immunodeficiency virus (HIV) infection or post-solid organ transplantation) and, even with aggressive treatment, prognosis remains poor. There is no definitive guideline for the treatment of PEL, although CHOP-like regimens (cyclophosphamide, doxorubicin, vincristine, and prednisone) are frequently prescribed and, given the rarity of this disease, therapeutic focus is being redirected to personalized and targeted approaches in the experimental realm. Current clinical trials include the combination of lenalidomide and rituximab into the EPOCH regimen and the treatment of individuals with relapsed/refractory EBV-associated disease with tabelecleucel.
MR Fingerprinting—A Radiogenomic Marker for Diffuse Gliomas
Authors: Springer Elisabeth , Cardoso Pedro Lima , Strasser Bernhard , Bogner Wolfgang , Preusser Matthias , Widhalm Georg , Nittka Mathias , Koerzdoerfer Gregor , Szomolanyi Pavol , Hangel Gilbert , Hainfellner Johannes A. , Marik Wolfgang , Trattnig Siegfried
Published: January 2022

(1) Background: Advanced MR imaging (MRI) of brain tumors is mainly based on qualitative contrast images. MR Fingerprinting (MRF) offers a novel approach. The purpose of this study was to use MRF-derived T1 and T2 relaxation maps to differentiate diffuse gliomas according to isocitrate dehydrogenase (IDH) mutation. (2) Methods: Twenty-four patients with histologically verified diffuse gliomas (14 IDH-mutant, four 1p/19q-codeleted, 10 IDH-wildtype) were enrolled. MRF T1 and T2 relaxation times were compared to apparent diffusion coefficient (ADC), relative cerebral blood volume (rCBV) within solid tumor, peritumoral edema, and normal-appearing white matter (NAWM), using contrast-enhanced MRI, diffusion-, perfusion-, and susceptibility-weighted imaging. For perfusion imaging, a T2* weighted perfusion sequence with leakage correction was used. Correlations of MRF T1 and T2 times with two established conventional sequences for T1 and T2 mapping were assessed (a fast double inversion recovery-based MR sequence (‘MP2RAGE’) for T1 quantification and a multi-contrast spin echo-based sequence for T2 quantification). (3) Results: MRF T1 and T2 relaxation times were significantly higher in the IDH-mutant than in IDH-wildtype gliomas within the solid part of the tumor (p = 0.024 for MRF T1, p = 0.041 for MRF T2). MRF T1 and T2 relaxation times were significantly higher in the IDH-wildtype than in IDH-mutant gliomas within peritumoral edema less than or equal to 1cm adjacent to the tumor (p = 0.038 for MRF T1 mean, p = 0.010 for MRF T2 mean). In the solid part of the tumor, there was a high correlation between MRF and conventionally measured T1 and T2 values (r = 0.913, p < 0.001 for T1, r = 0.775, p < 0.001 for T2), as well as between MRF and ADC values (r = 0.813, p < 0.001 for T2, r = 0.697, p < 0.001 for T1). The correlation was weak between the MRF and rCBV values (r = −0.374, p = 0.005 for T2, r = −0.181, p = 0.181 for T1). (4) Conclusions: MRF enables fast, single-sequence based, multi-parametric, quantitative tissue characterization of diffuse gliomas and may have the potential to differentiate IDH-mutant from IDH-wildtype gliomas.
CONUT Score: A New Tool for Predicting Prognosis in Patients with Advanced Thyroid Cancer Treated with TKI
Authors: Dalmiglio Cristina , Brilli Lucia , Campanile Michele , Ciuoli Cristina , Cartocci Alessandra , Castagna Maria Grazia
Published: January 2022

(1) Background: The Controlling Nutritional Status (CONUT) score is an immuno-nutritional screening tool based on serum albumin, total cholesterol, and lymphocyte count. The aim of the study was to assess the CONUT score as a potential prognostic factor of response to therapy in patients with advanced thyroid cancer treated with tyrosine kinase inhibitors (TKIs). (2) Methods: We retrospectively evaluated 42 metastatic thyroid cancer patients (54.8% female). The median age at the time of TKI treatment was 69 years. Histological diagnosis was differentiated thyroid cancer in 66.7%, poorly differentiated thyroid cancer in 21.4%, and medullary thyroid cancer in 11.9% of patients. CONUT score was assessed before starting TKI therapy. (3) Results: Progression-free survival (PFS) and overall survival (OS) were significantly influenced by baseline CONUT score. The best CONUT cut-off able to predict the response to treatment was 3. Both PFS and OS were better in patients with CONUT score <3 than in those with CONUT score ≥3 (p < 0.0001). CONUT score was the only independent prognostic factor associated with PFS (p = 0.021) and OS (p = 0.007). (4) Conclusions: CONUT score represents a relatively new screening tool, easily applicable in clinical practice and potentially useful in predicting prognosis in thyroid cancer patients treated with TKIs.
Surgery for Hereditary Diffuse Gastric Cancer: Long-Term Outcomes
Authors: Forrester Joseph D. , Foster Deshka , Ford James M. , Longacre Teri A. , Ladabaum Uri , Fry Sara , Norton Jeffrey A.
Published: January 2022

Introduction: Gastric cancer is inherited as an autosomal dominant condition in hereditary diffuse gastric cancer (HDGC). The gene associated with HDGC is an E-cadherin gene CDH1. At the time of initiation of this study, it was estimated that 70% of patients who inherited the CDH1 gene mutation would develop gastric cancer. We hypothesized that the rate of signet ring cell cancer in asymptomatic patients with CDH1 mutations may be higher than anticipated and that the surgery could be conducted with acceptable short-term and long-term complications suggesting that the quality of life with the surgery is acceptable. Methods: We prospectively studied the role of total gastrectomy in symptomatic and asymptomatic patients with CDH1 mutations. A total of 43 patients with mutations of the CDH1 gene were studied prospectively, including 8 with symptoms and 35 without symptoms. Total gastrectomy was recommended to each. Quality of life was assessed in patients who underwent prophylactic gastrectomy. Proportions are compared with Fisher’s exact test. Results: In total, 13 (30%) asymptomatic patients declined surgery. Total gastrectomy was performed in 8 symptomatic patients and 22 asymptomatic patients of whom only 3 asymptomatic patients (14%) had endoscopically proven signet ring cell cancer preoperatively, while 21 of 22 (95%) had it on final pathology (p = 0.05). Each asymptomatic patient was T1, N0, while seven out of eight symptomatic patients had T3-T4 tumors and six had positive lymph nodes. None had operative complications or operative death. The median follow-up was 7 years. Five (63%) symptomatic patients died, while only one (95%) prophylactic patient died of a non-gastric cancer- or surgery-related issue (p = 0.05). A total of 15 prophylactic patients had long-term follow-up. Each had significant weight loss (mean 23%) but all had a normal body mass index. In total, 40% had bile reflux gastritis controlled with sucralfate. Each returned to work and, if given the choice, said that they would undergo the surgery again. Conclusions: Total gastrectomy is indicated for patients who have an inherented CDH1 mutation. Endoscopic screening is not reliable for diagnosing signet ring cell stomach cancer. If patients wait for symptoms, they will have a more advanced disease and significantly reduced survival. Operative complications of prophylactic gastrectomy are minimal, and long-term quality of life is acceptable.
Loss of MAGEC3 Expression Is Associated with Prognosis in Advanced Ovarian Cancers
Authors: Ellegate James , Mastri Michalis , Isenhart Emily , Krolewski John J. , Chatta Gurkamal , Kauffman Eric , Moffitt Melissa , Eng Kevin H.
Published: January 2022

Rare variants in MAGEC3 are associated with BRCA negative, early-onset ovarian cancers. Given this association, we evaluated the impact of MAGEC3 protein expression on prognosis and transcription. We quantified normal and tumor protein expression of MAGEC3 via immunohistochemistry in n = 394 advanced ovarian cancers, assessed the correlation of these values with clinicopathologic and immunological features and modeled survival using univariate and multivariate models. To extend these results, we quantified MAGEC3 protein expression in n = 180 cancers and used matching RNA sequencing data to determine MAGEC3-associated differentially expressed genes and to build an RNA-based model of MAGEC3 protein levels. This model was tested in a third independent cohort of patients from TCGA’s OV dataset (n = 282). MAGEC3 protein was sporadically lost in ovarian cancers, with half of the cases falling below the 9.5th percentile of normal tissue expression. Cases with MAGEC3 loss demonstrated better progression-free survival [HR = 0.71, p = 0.004], and analyses performed on predicted protein scores were consistent [HR = 0.57 p = 0.002]. MAGEC3 protein was correlated with CD8 protein expression [Pearson’s r = 0.176, p = 0.011], NY-ESO-1 seropositivity, and mRNA expression of tumor antigens at Xq28. Results of gene set enrichment analysis showed that genes associated with MAGEC3 protein expression cluster around G2/M checkpoint (NES = 3.20, FDR < 0.001) and DNA repair (NES = 2.28, FDR < 0.001) hallmark pathways. These results show that MAGEC3 is a prognostic biomarker in ovarian cancer.
Utility of Cell-Free DNA Detection in Transplant Oncology
Authors: Reddy Tejaswini , Esmail Abdullah , Chang Jenny C. , Ghobrial Rafik Mark , Abdelrahim Maen
Published: January 2022

Transplant oncology is an emerging field in cancer treatment that applies transplant medicine, surgery, and oncology to improve cancer patient survival and quality of life. A critical concept that must be addressed to ensure the successful application of transplant oncology to patient care is efficient monitoring of tumor burden pre-and post-transplant and transplant rejection. Cell-free DNA (cfDNA) detection has emerged as a vital tool in revolutionizing the management of cancer patients who undergo organ transplantation. The advances in cfDNA technology have provided options to perform a pre-transplant evaluation of minimal residual disease (MRD) and post-transplant evaluation of cancer recurrence and transplant rejection. This review aims to provide a comprehensive overview of the history and emergence of cfDNA technology, its applications to specifically monitor tumor burden at pre-and post-transplant stages, and evaluate transplant rejection.
Male Pheromones Induce Ovulation in Female Honeycomb Groupers (Epinephelus merra): A Comprehensive Study of Spawning Aggregation Behavior and Ovarian Development
Authors: Amagai Takafumi , Izumida Daisuke , Murata Ryosuke , Soyano Kiyoshi
Published: January 2022

This study characterizes the spawning phenomena of the honeycomb grouper (Epinephelus merra), which is a lunar-synchronized spawner that spawns a few days after full moon. To elucidate the aggregation characteristics of wild honeycomb groupers, the numbers of males and females at the spawning grounds were counted before and after the full moon. Approximately 20 males were consistently observed at the spawning grounds throughout the study period. Females appeared several days after full moon and rapidly increased in number, peaking four days after full moon (41 individuals). The maturation status of the females aggregating at the spawning grounds was investigated. The gonadosomatic index increased rapidly three days after full moon, and ovulation was confirmed. Individuals with ovulatory eggs were present for three days, after which the number of females at the spawning grounds decreased. Additionally, the role of males in final oocyte maturation (FOM) and ovulation in females during the spawning phase was investigated in captivity. FOM was induced in females reared in water with mature males, suggesting that male pheromones in the water induced FOM via activation of the hypothalamic–pituitary–gonadal axis. This suggests that spawning at the natural spawning grounds was the result of male–female interactions via pheromones.
Detection of Single Nucleotide Polymorphism (SNP) Variation of a Gene Sequence on Membrane-Based Lateral-Flow Strips
Authors: Wu Jui-Chuang , Yang Jia-Cheng , Hsieh Yi-Jing
Published: January 2022

This study used appropriate primers to distinguish the gene model, HLA-A31:01, on membrane-based lateral-flow (MBLF) strips from its allele, which is with an SNP. Using primers designed with a mismatch base on one or two sides next to the SNP spot was verified as a good approach. In the optimal condition, the detection limits of 1~0.1 ng/μL nucleotides were in agreement with reports in the literature, and the intra- and inter-assay tests ensured the detection reproducibility of this approach with CV% of 2.5~15.9% and 1.7~14.7%, respectively. The detection specificity was also validated by the tests on the selected negative-control genes. The tests on MBLF strips in this study showed an easy, robust, reproducible, and reliable detection methodology for untrained personnel at care points with limited instrument and particularly for avoiding medications from faulty prescriptions.
Advanced Ultrasound Techniques for Neuroimaging in Pediatric Critical Care: A Review
Authors: Freeman Colbey W. , Hwang Misun
Published: January 2022

Because of its portability, safety profile, and accessibility, ultrasound has been integral in pediatric neuroimaging. While conventional B-mode and Doppler ultrasound provide anatomic and limited flow information, new and developing advanced ultrasound techniques are facilitating real-time visualization of brain perfusion, microvascular flow, and changes in tissue stiffness in the brain. These techniques, which include contrast-enhanced ultrasound, microvascular imaging, and elastography, are providing new insights into and new methods of evaluating pathologies affecting children requiring critical care, including hypoxic–ischemic encephalopathy, stroke, and hydrocephalus. This review introduces advanced neurosonography techniques and their clinical applications in pediatric neurocritical care.
Patterns of Medication Prescription among Children and Adolescents with Attention-Deficit/Hyperactivity Disorder in the United States
Authors: Meraya Abdulkarim M.
Published: January 2022

The objectives of this study are to: (1) quantify the difference in the annual number of prescription medications (total and unique) between children and adolescents with ADHD and those without ADHD; and (2) identify the most prescribed medication classes and unique medications among children and adolescents with ADHD. A retrospective cross-sectional study design was employed using data from the 2015 and 2017 Medical Expenditure Panel Survey. The study sample comprised children and adolescents (5–17 years). In the 5–12-year age group, those with ADHD were 2.4%, 17%, and 15% significantly more likely to have one, 2–4, and ≥5 prescription medications, respectively. Similarly, those in the 13–17-year age group were more likely to have one prescription medication (3%), 2–4 prescription medications (15%), and ≥5 prescription medications (12%) than those without ADHD. The most prescribed medications among children and adolescents with ADHD were methylphenidate and amphetamine-dextroamphetamine. ADHD was associated with both higher annual total and unique prescription medications. Additionally, concurrent use of prescription medications was more prevalent among children and adolescents with ADHD. High-quality randomized clinical trials on the safety and efficacy of combinations of multiple psychotherapeutics and stimulants’ agents are required to guide the evidence-based practices.
Microstructural Effects on Thermal-Mechanical Alleviation of Cold Dwell Fatigue in Titanium Alloys
Authors: Shen Songlin , Zhan Mei , Gao Pengfei , Hao Wenshuo , Dunne Fionn P. E. , Zheng Zebang
Published: January 2022

Cold dwell fatigue is a well-known problem in the titanium components of aircraft engines. The high temperature and low dwell stress of in-service conditions have been reported to give rise to dwell fatigue resistance through a thermal-mechanical alleviation process. Here, dwell fatigue tests at room temperature and the component operating temperature were performed on IMI834 titanium alloy to assess the microstructural effects on thermal-mechanical alleviation of cold dwell fatigue while eliminating the effect of chemical composition. The ratcheting strain rates under different loading conditions were quantitatively investigated to aid the understanding of thermal-mechanical alleviation.
Microstructure, Mechanical and Thermal Properties of Mg-0.5Ca-xZr Alloys
Authors: Zhou Ying-Long , Liu Jie , Luo Dong-Mei
Published: January 2022

To evaluate the potential values in heat dissipation applications, this study investigated the microstructure, mechanical and thermal properties of the Mg-0.5Ca-xZr alloys (x = 0.5 and 1 wt.%) under the as-cast, as-extruded and aged states. The phase constituents of the Mg alloys were examined by X-ray diffraction analysis, and the microstructure was inspected by optical microscopy and scanning electron microscopy. The thermal conductivity and mechanical properties of the Mg alloys were measured by the laser flash method and tensile tests, respectively. The results showed that the Mg alloys exhibited the equiaxed microstructure which is composed of α-Mg, Zr and compound Mg2Ca. Both the extrusion process and increase of Zr content remarkably enhanced the mechanical strength of the Mg alloys and deteriorated the thermal performance simultaneously. It was also found that the thermal conductivity and mechanical strength of the Mg alloys increased gradually with the increase of aging time due to the higher precipitation of Zr and compound Mg2Ca during the aging treatment. TheMg-0.5Ca-0.5Zr alloy aged at 473 K for 48 h demonstratedhigher thermal conductivity than the required values of the Mg alloys used as heat dissipation materials. Moreover, theMg-0.5Ca-0.5Zr alloy exhibited similar mechanical strength to the commonly-used Mg alloys, highlighting its potential become a potential heat dissipation material in the future due to its good combination of high thermal performance and mechanical strength.
Substantially Enhanced Stereocomplex Crystallization of Poly(L-lactide)/Poly(D-lactide) Blends by the Formation of Multi-Arm Stereo-Block Copolymers
Authors: Diao Xingyuan , Chen Xiaonan , Deng Shihao , Bai Hongwei
Published: January 2022

Stereocomplex-type polylactide (SC-PLA) created by alternate packing of poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA) chains in a crystalline state has emerged as a growingly popular engineering bioplastic that possesses excellent hydrolytic stability and thermomechanical properties. However, it is extremely difficult to acquire high-performance SC-PLA products via melt-processing of high-molecular-weight PLLA/PDLA blends because both SC crystallites and homocrystallites (HCs) are competitively formed in the melt-crystallization. Herein, a facile yet powerful way was employed to boost SC formation by introducing trace amounts of some epoxy-functionalized small-molecule modifiers into the enantiomeric blends during reactive melt-blending. The results show that the SC formation is considerably enhanced with the in situ generation of multi-arm stereo-block PLA copolymers, based on the reaction between epoxy groups of the modifiers and hydroxyl end groups of PLAs. More impressively, it is intriguing to find that the introduction of only 0.5 wt% modifiers can induce exclusive SC formation in the blends upon isothermal and non-isothermal melt-crystallizations. The outstanding SC crystallizability might be attributed to the suppressing effect of such unique copolymers on the separation of the alternately arranged PLLA/PDLA chain segments in molten state as a compatibilizer. Furthermore, the generation of these copolymers does not result in a significant increase in melt viscosity of the blends. These findings suggest new opportunities for the high-throughput processing of SC-PLA materials into useful products.
Passivation of Surface States in GaN by NiO Particles
Authors: Velazquez-Rizo Martin , Kirilenko Pavel , Iida Daisuke , Zhuang Zhe , Ohkawa Kazuhiro
Published: January 2022

GaN and NiO/GaN electrodes were characterized by impedance spectroscopy measurements in 0.1 M NaOH. We observed the suppression of the surface states capacitance due to the modification of the chemical state of superficial Ga atoms by NiO. This result suggests that the carriers involved in the photocorrosion of GaN in alkaline conditions originate in its surface states. In addition, we characterized the epitaxial relationship between the NiO particles deposited on GaN by transmission electron microscopy, finding the NiO{111}||GaN{0002} and NiO[220] ||GaN[112¯0] symmetry constraints.
Performance Enhancement of Self-Cleaning Cotton Fabric with ZnO NPs and Dicarboxylic Acids
Authors: Ji Xinlei , Li Hong , Qin Yuan , Yan Jun
Published: January 2022

In this paper, we explore the self-cleaning and washing durability of green-prepared ZnO NPs combined with cotton fabrics. Honeysuckle extract was used to prepare ZnO NPs with an average particle size of 15.3 nm. Cotton fabrics were then treated with oxalic acid (OA), tartaric acid (TA), and succinic acid (SA) as cross-linking agents, sodium hypophosphite as a catalyst, and after that, the ZnO NPs were applied to the cross-linked cotton fabrics by the padding to prepare the self-cleaning cotton fabrics. The morphology and structure of the fabric samples were characterized using FTIR, scanning electron microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDS), and XRD. The optical properties of the cotton fabric samples were discussed by UV-vis diffuse reflectance spectrum, and the self-cleaning performance, wrinkle recovery angle and ultraviolet protection performance of the cotton fabric samples were analyzed. The results showed that the carboxyl groups of TA, OA, and SA were esterified with hydroxyl groups of the cotton fiber and formed a film on the surface of the cotton fabrics. ZnO NPs were successfully loaded onto the cotton fabrics by strong electrostatic interaction, causing the improvement of the washing resistance of the cross-linked fabrics. In addition, compared with uncross-linked fabrics, the wrinkle recovery performance of the cross-linked fabrics had also been greatly improved, and the UV protection factor reached 50+, thus obtaining an excellent self-cleaning, multifunctional cotton-based textile with anti-wrinkle and anti-ultraviolet properties.
Endometrial Cancer: Transitioning from Histology to Genomics
Authors: Mitric Cristina , Bernardini Marcus Q.
Published: January 2022

Endometrial carcinoma (EC) is traditionally treated with surgery and adjuvant treatment depending on clinicopathological risk factors. The genomic analysis of EC in 2013 and subsequent studies using immunohistochemistry have led to the current EC molecular classification into: polymerase epsilon mutated (POLEmut), p53 abnormal (p53abn), mismatch repair deficient (MMRd), and no specific molecular profile (NSMP). The four groups have prognostic value and represent a promising tool for clinical decision-making regarding adjuvant treatment. Molecular classification was integrated into the recent European Society of Gynecologic Oncology (ESGO) management guidelines. POLEmut EC has favorable outcomes and retrospective studies found that omitting adjuvant treatment is safe in this group; two prospective clinical trials, PORTEC-4 and TAPER, are ongoing to assess this. p53 abn is associated with increased recurrence, decreased survival, and benefitted from chemotherapy in the PORTEC-3 subgroup molecular analysis. The clinical trials PORTEC-4a and CANSTAMP will prospectively assess this. MMRd and NSMP groups have intermediate prognosis and will likely continue to rely closely on clinicopathological features for adjuvant treatment decisions. In addition, the molecular classification has led to exploring novel treatments such as checkpoint inhibitors. Overall, the molecular perspective on EC and associated clinical trials will likely refine EC risk stratification to optimize care and avoid overtreatment.