bluuebunny/crossref_metadata_2025 huggingface dataset:
Classification and Assessment of Core Fractures in a Post-Fracturing Conglomerate Reservoir Using the AHP–FCE Method
Authors: Zhuo Renyan , Ma Xinfang , Zhang Shicheng , Ma Junxiu , Xiang Yuankai , Sun Haoran
Published: December 2022

To characterize the hydraulic fracture network of a conglomerate reservoir, a slant core well was drilled aimed to obtain direct information regarding hydraulic fractures through slant core at the conglomerate hydraulic fracturing test site (CHFTS). Core fracture classification was the fundamental issue of the project. In this study, three grade classifications for core fractures were proposed. Comprehensive classification of core fractures was carried out using the analytic hierarchy process (AHP)–fuzzy comprehensive evaluation (FCE) method. Finally, the fracture classification results were validated against numerical simulation. The grade-1 fracture classification included hydraulic fractures, drilling-induced fractures and core cutting-induced fractures. A total of 214 hydraulic fractures were observed. For the grade-2 classification, the hydraulic fractures were divided into 47 tensile fractures and 167 shear fractures. For the grade-3 classification, the shear fractures were subdivided into 45 tensile-shear fractures and 122 compression-shear fractures. Based on the numerical verification of the core fracture classifications, the dataset acquired was applied to analyze the spatial distribution of tensile and shear fractures. Results showed that the tensile fractures were mainly in the near-wellbore area with lateral distances of less than 20–25 m from the wellbore. The shear fractures were mainly in the far-wellbore area with lateral distances of 20–30 m from the wellbore. These results provide a basis for understanding the fracture types, density, and failure mechanisms of post-fracturing conglomerate reservoir.
Methods for Reducing Cogging Force in Permanent Magnet Machines: A Review
Authors: Hao Wenjuan , Zhang Gong , Liu Wenbo , Liu Hui , Wang Yu
Published: December 2022

Permanent magnet (PM) machines inevitably suffer from cogging force, which does not contribute to the average output torque (force) but contributes as a type of torque (force) ripple. This paper provides an overview of the cogging force reduction methods for different types of PM machines. First, a systematic and comprehensive categorization of different kinds of cogging force reduction methods is given according to the reduction principle. Then, the cogging force reduction methods for different types of PM machines are analyzed and discussed based on the categorization. Finally, according to the versatility and feasibility of the cogging force reduction methods, practical methods are recommended for different types of PM machines. The categorization, analyses, and recommendations presented in the paper are useful for the design of different types of PM machines with the requirement of cogging force reduction or output torque (force) ripple suppression.
Okara Waste as a Substrate for the Microalgae Phaeodactylum tricornutum Enhances the Production of Algal Biomass, Fucoxanthin, and Polyunsaturated Fatty Acids
Authors: Kim Jaejung , Lee Jaslyn , Voo Amanda Ying Hui , Tan Yong Xing , Mok Wai Kit , Li Aaron Zongwei , Chen Wei Ning
Published: December 2022

Despite the rich nutritional content of okara, the majority remains underutilized and discarded as food waste. In this study, solid-state fermentation of okara with food-grade fungi was performed to extract and solubilize any remnant nutrients locked within the lignocellulosic matrix to produce a nutrient-rich okara fermentate. Fermented okara media (FOM) was used as the sole nutrient source for growing marine diatom, Phaeodactylum tricornutum. Results have shown a two-fold increase in biomass production when grown on FOM (0.52 g L−1) as compared with conventional Guillard’s F/2 media (0.25 g L−1). Furthermore, cellular fucoxanthin content was enhanced significantly by two-fold to reach a final concentration of 15.3 mg g−1 compared to 7.3 mg g−1. Additionally, a significantly higher amount of polyunsaturated fatty acid (PUFA) was produced, particularly eicosapentaenoic acid (EPA) which yield has increased by nearly three-fold. Metabolomics analysis of intracellular contents in fermented okara culture revealed a significantly enhanced accumulation of nitrogenous metabolites, alongside the decrease in sugar metabolites as compared to F/2 culture, thus indicating metabolic flux towards pathways involved in cellular growth. This study demonstrated an innovative and low-cost strategy of using fermented okara as a nutritious substrate for achieving a sustainable media replacement for high density algal growth with a simultaneous enhancement of production in highly valued nutraceuticals, including fucoxanthin and EPA.
Evacuation Simulation in a Cultural Asset Fire: Impact of Fire Emergency Evacuation Facilities for People with Disabilities on Evacuation Time
Authors: Hu Jyh-Jye , Wu Hao-Yu , Chou Chung-Chyi
Published: December 2022

More cautious fire safety evacuation assessment and escape route assistance are required when people with disabilities encounter a fire hazard in a historical museum. This study uses the old Chiayi City Hall, which is mainly used for an exhibition space, as the example. The core of this study is the problem of fire evacuation that emerged after the historic building opened and was reused as a museum; it uses Pathfinder evacuation simulation software, discussing the difference between traditional and segregated evacuation and assuming the original evacuation, elevator emergency evacuation, and external ramp evaluation. There are three evacuation scenarios, and the number of accommodated people is set to 730, pursuant to the applicable law. Comparing the conditions of people with disabilities and ordinary members of the public during evacuation through the foregoing three scenarios, the overall evacuation time and survival rate of the original evacuation scenario are 440 s and 49.8%, respectively; and the overall evacuation time and survival rate of the barrier-free elevator emergency evacuation scenario are 332 s and 65.4%, respectively; the overall evacuation time and survival rate of the external ramp evaluation scenario are 320 s and 65.6%, respectively. The computer data analysis shows that the use of the external ramp evaluation gives people with disabilities the best evacuation time and survival rate because the architectural form of cultural heritage buildings is more fragile, specific, and fast-burning than that of ordinary buildings. As the global awareness of cultural asset preservation and revitalization is increasing, the evacuation of people with disabilities in the building in the case of fire is very important. The results of this study can be used as an emergency evacuation design recommendation for people with disabilities in the cultural assets through evacuation simulation analysis.
Patterning Behavior of Hybrid Buoyancy-Marangoni Convection in Inclined Layers Heated from Below
Authors: Waris Wasim , Lappa Marcello
Published: December 2022

Alongside classical effects driven by gravity or surface tension in non-isothermal fluids, the present experimental study concentrates on other exotic (poorly known) modes of convection, which are enabled in a fluid layer delimited from below by a hot plate and unbounded from above when its container is inclined to the horizontal direction. By means of a concerted approach based on the application of a thermographic visualization technique, multiple temperature measurements at different points and a posteriori computer-based reconstruction of the spatial distribution of wavelengths, it is shown that this fluid-dynamic system is prone to develop a rich set of patterns. These include (but are not limited to), spatially localized (compact) cells, longitudinal wavy rolls, various defects produced by other instabilities and finger-like structures resulting from an interesting roll pinching mechanism (by which a single longitudinal roll can be split into two neighboring rolls with smaller wavelength). Through parametric variation of the tilt angle, the imposed temperature difference and the volume of liquid employed, it is inferred that the observable dynamics are driven by the ability of gravity-induced shear flow to break the in-plane isotropy of the system, the relative importance of surface-tension-driven and buoyancy effects, and the spatially varying depth of the layer. Some effort is provided to identify universality classes and similarities with other out-of-equilibrium thermal systems, which have attracted significant attention in the literature.
Influence of Anticaking Agents and Storage Conditions on Quality Characteristics of Spray Dried Apricot Powder: Shelf Life Prediction Studies Using Guggenheim-Anderson-de Boer (GAB) Model
Authors: Bashir Omar , Hussain Syed Zameer , Ameer Kashif , Amin Tawheed , Beenish  , Ahmed Isam A. Mohamed , Aljobair Moneera O. , Gani Gousia , Mir Shakeel Ahmad , Ayaz Qudsiya , Nazir Nowsheen
Published: December 2022

Apricot powder was developed through spray drying using gum arabic as an encapsulating material at a concentration of 19%. Inlet air temperature, feed total soluble solids (TSS), feed flow rate, and atomization speed were 190 °C, 23.0 °C, 300.05 mL/h, and 17,433 rpm, respectively. This study was therefore conducted to investigate the influence of anticaking agents (tricalcium phosphate and silicon dioxide) and storage conditions (ambient and accelerated) on physicochemical, micrometric, and thermal characteristics of spray-dried apricot powder (SDAP) packaged in aluminum laminates. Both tricalcium phosphate (TCP) and silicon dioxide (SiO2) improved the shelf life and quality of SDAP, with TCP being more effective, since a lower increase in water activity (aw), moisture content, degree of caking, hygroscopicity, and rehydration time was observed in TCP-treated samples followed by SiO2-treated samples than the control. Furthermore, flowability, glass transition temperature (Tg), and sticky-point temperature (Ts) of SDAP tended to decrease in a significant manner (p < 0.05) under both storage conditions. However, the rate of decrease was higher during accelerated storage. The water activity of treated samples under ambient conditions did not exceed 0.60 and had a total plate count within the permissible range of 40,000 CFU/g, indicating shelf stability of the powder. The predicted shelf life of powder obtained from the Guggenheim–Anderson–de Boer (GAB) model and experimental values were very similar, with TCP-treated samples having a predicted shelf life of 157 days and 77 days under ambient and accelerated storage conditions, respectively. However, the respective experimental shelf life under the same conditions was 150 and 75 days, respectively. Similarly, the predicted shelf life of SiO2-treated samples under ambient and accelerated storage was 137 and 39 days, respectively, whereas the experimental values were 148 and 47 days, respectively. In conclusion, TCP proved more effective than SiO2 at preserving shelf life by preventing moisture ingress.
The Right Equivalent Integral Equation of Impulsive Caputo Fractional-Order System of Order ϵ∈(1,2)
Authors: Zhang Xianmin , Liu Zuohua , Yang Shixian , Peng Zuming , He Yali , Wei Liran
Published: December 2022

For the impulsive fractional-order system (IFrOS) of order ϵ∈(1,2), there have appeared some conflicting equivalent integral equations in existing studies. However, we find two fractional-order properties of piecewise function and use them to verify that these given equivalent integral equations have some defects to not be the equivalent integral equation of the IFrOS. For the IFrOS, its limit property shows the linear additivity of the impulsive effects. For the IFrOS, we use the limit analysis and the linear additivity of the impulsive effects to find its correct equivalent integral equation, which is a combination of some piecewise functions with two arbitrary constants; that is, the solution of the IFrOS is a general solution. Finally, a numerical example is given to show the equivalent integral equation and the non-uniqueness of the solution of the IFrOS.
New Results for Homoclinic Fractional Hamiltonian Systems of Order α∈(1/2,1]
Authors: Moumen Abdelkader , Boulares Hamid , Alzabut Jehad , Khelifi Fathi , Imsatfia Moheddine
Published: December 2022

In this manuscript, we are interested in studying the homoclinic solutions of fractional Hamiltonian system of the form −D∞ας(Dςα−∞Z(ς))−A(ς)Z(ς)+∇ω(ς,Z(ς))=0, where α∈(12,1], Z∈Hα(R,RN) and ω∈C1(R×RN,R) are not periodic in ς. The characteristics of the critical point theory are used to illustrate the primary findings. Our results substantially improve and generalize the most recent results of the proposed system. We conclude our study by providing an example to highlight the significance of the theoretical results.
Existence Results for Nonlinear Fractional Differential Inclusions via q-ROF Fixed Point
Authors: Shahid Lariab , Rashid Maliha , Azam Akbar , Ali Faryad
Published: December 2022

Fractional Differential inclusions, the multivalued version of fractional differential equations, yellow play a vital role in various fields of applied sciences. In the present article, a class of q-rung orthopair fuzzy (q-ROF) set valued mappings along with q-ROF upper/lower semi-continuity have been introduced. Based on these ideas, existence theorems for a numerical solution of a distinct class of fractional differential inclusions have been achieved with the help of Schaefer type and Banach contraction fixed point theorems. A physical example is also provided to validate the hypothesis of the main results. The notion of q-rung orthopair fuzzy mappings along with the use of fixed point techniques and a new-fangled Caputo type fractional derivative are the principal novelty of this article.
Comparative Effects of Mulligan’s Mobilization, Spinal Manipulation, and Conventional Massage Therapy in Cervicogenic Headache—A Prospective, Randomized, Controlled Trial
Authors: Nambi Gopal , Alghadier Mshari , Ebrahim Elturabi Elsayed , Vellaiyan Arul , Tedla Jaya Shanker , Reddy Ravi Shankar , Kakaraparthi Venkata Nagaraj , Aldhafian Osama R. , Alshahrani Naif N. , Saleh Ayman K.
Published: December 2022

Background: There is ample evidence supporting the use of manual therapy techniques for the treatment of cervicogenic headache (CGH). Objective: The objective of this study was to find and compare the effects of different manual therapy approaches to cervicogenic headache. Methods: A randomized, controlled study was conducted on 84 CGH participants at the university hospital. The participants were divided into a Mulligan mobilization therapy group (MMT; n = 28), a spinal manipulation therapy group (SMT; n = 28), and a control group (Control; n = 28); they received the respective treatments for four weeks. The primary outcome (CGH frequency) and secondary outcomes (CGH pain intensity, CGH disability, neck pain frequency, pain intensity, pain threshold, flexion rotation (right and left), neck disability index, and quality of life scores) were measured at baseline, after 4 weeks, after 8 weeks, and at a 6-month follow-up. The one-way ANOVA test and repeated measures analysis of variance (rANOVA) test were performed to find the difference between the inter- and intra-treatment group effects. Results: Four weeks following training, the MMT group showed a statistically significant difference in the primary (CGH frequency) and secondary (CGH pain intensity, CGH disability, neck pain frequency, neck pain intensity, flexion rotation test, neck disability index, and quality of life) scores than those of the SMT and control groups (p < 0.001). The same difference was seen in the above variables at 8 weeks and at the 6-month follow-up. At the same time, the neck pain threshold level did not show any difference at the 4-week and the 8-week follow-up (p ≥ 0.05) but showed statistical difference at the 6-month follow-up. Conclusion: The study concluded that Mulligan’s mobilization therapy provided better outcomes in cervicogenic headache than those of spinal manipulation therapy and conventional massage therapy.
Perspectives of Healthcare Providers towards Remote Medical Interpreting Services in Japan
Authors: Saeki Soichiro , Minamitani Kaori , Iwaoka Fumika , Shirai Kokoro
Published: December 2022

Language support is necessary for effective healthcare as language obstacles have a negative impact on patient outcomes. Medical facilities dealing with novel coronavirus disease (COVID-19) were forced to restrict the number of healthcare professionals on the field, and medical interpreters were no exception. This has prompted the introduction of remote medical interpreting systems, which do not necessitate the presence of an interpreter onsite. However, as the dominant trend in offering linguistic help was face-to-face medical interpreting, healthcare staff are also battling with its utilization. We conducted a single-centered, retrospective study by examining written responses taken from April 2018 to March 2020 and a total of 236 healthcare employees in Japan, to identify the primary reasons of such challenges. Remote medical interpreting was frequently employed by a range of professions in many departments across various languages, and the majority of users were satisfied with the experience. The qualitative analysis based on the free opinions of the healthcare professionals unraveled three main concerns towards remote medical interpreting: connection to the interpreting providers; coordination of the remote interpreting coordinators, and quality of interpreting. Therefore, increasing the use of remote medical interpreting while simultaneously training interpreters by enhancing the skills required in Japanese medical facilities would be necessary.
Artificial-Intelligence-Based Decision Making for Oral Potentially Malignant Disorder Diagnosis in Internet of Medical Things Environment
Authors: Alabdan Rana , Alruban Abdulrahman , Hilal Anwer Mustafa , Motwakel Abdelwahed
Published: December 2022

Oral cancer is considered one of the most common cancer types in several counties. Earlier-stage identification is essential for better prognosis, treatment, and survival. To enhance precision medicine, Internet of Medical Things (IoMT) and deep learning (DL) models can be developed for automated oral cancer classification to improve detection rate and decrease cancer-specific mortality. This article focuses on the design of an optimal Inception-Deep Convolution Neural Network for Oral Potentially Malignant Disorder Detection (OIDCNN-OPMDD) technique in the IoMT environment. The presented OIDCNN-OPMDD technique mainly concentrates on identifying and classifying oral cancer by using an IoMT device-based data collection process. In this study, the feature extraction and classification process are performed using the IDCNN model, which integrates the Inception module with DCNN. To enhance the classification performance of the IDCNN model, the moth flame optimization (MFO) technique can be employed. The experimental results of the OIDCNN-OPMDD technique are investigated, and the results are inspected under specific measures. The experimental outcome pointed out the enhanced performance of the OIDCNN-OPMDD model over other DL models.
Early-Life Exposure to Traffic-Related Air Pollutants Induced Anxiety-like Behaviors in Rats via Neurotransmitters and Neurotrophic Factors
Authors: Kyi-Tha-Thu Chaw , Fujitani Yuji , Hirano Seishiro , Win-Shwe Tin-Tin
Published: December 2022

Recent epidemiological studies have reported significantly increasing hospital admission rates for mental disorders such as anxiety and depression, not only in adults but also in children and adolescents, indicating more research is needed for evaluation of the etiology and possible reduction and prevention of these disorders. The aim of the present study was to examine the associations between perinatal exposure to traffic-related air pollutants and anxiety-like behaviors and alterations in neurological and immunological markers in adulthood using a rat model. Sprague Dawley pregnant rats were exposed to clean air (control), diesel exhaust (DE) 101 ± 9 μg/m3 or diesel exhaust origin secondary organic aerosol (DE-SOA) 118 ± 23 μg/m3 from gestational day 14 to postnatal day 21. Anxiety-related behavioral tests including open field tests, elevated plus maze, light/dark transition tests and novelty-induced hypophagia were performed on 10-week-old rats. The hippocampal expression of neurotransmitters, neurotrophic factors, and inflammatory molecular markers was examined by real-time RT-PCR. Anxiety-like behaviors were observed in both male and female rat offspring exposed to DE or DE-SOA. Moreover, serotonin receptor (5HT1A), dopamine receptor (Drd2), brain-derived neurotrophic factor and vascular endothelial growth factor A mRNAs were significantly decreased, whereas interleukin-1β, cyclooxygenase-2, heme oxygenase-1 mRNAs and microglial activation were significantly increased in both male and female rats. These findings indicate that brain developmental period exposure to traffic-related air pollutants may induce anxiety-like behaviors via modulation of neurotransmitters, neurotrophic factors, and immunological molecular markers, triggering neuroinflammation and microglia activation in rats.
Characterization of Hyaluronic Acid-Coated PLGA Nanoparticles by Surface-Enhanced Raman Spectroscopy
Authors: La Verde Giuseppe , Sasso Antonio , Rusciano Giulia , Capaccio Angela , Fusco Sabato , Mayol Laura , Biondi Marco , Silvestri Teresa , Netti Paolo A. , La Commara Marco , Panzetta Valeria , Pugliese Mariagabriella
Published: December 2022

Nanoparticles (NPs) coated with hyaluronic acid (HA) seem to be increasingly promising for targeted therapy due to HA chemical versatility, which allows them to bind drugs of different natures, and their affinity with the transmembrane receptor CD-44, overexpressed in tumor cells. However, an essential aspect for clinical use of NPs is formulation stability over time. For these reasons, analytical techniques capable of characterizing their physico-chemical properties are needed. In this work, poly(lactide-co-glycolide) (PLGA) NPs with an average diameter of 100–150 nm, coated with a few 10 s of nm of HA, were synthesized. For stability characterization, two complementary investigative techniques were used: Dynamic Light Scattering (DLS) and Surface-Enhanced Raman Scattering (SERS) spectroscopy. The first technique provided information on size, polidispersity index, and zeta-potential, and the second provided a deeper insight on the NP surface chemicals, allowing distinguishing of HA-coated NPs from uncoated ones. Furthermore, in order to estimate formulation stability over time, NPs were measured and monitored for two weeks. SERS results showed a progressive decrease in the signal associated with HA, which, however, is not detectable by the DLS measurements.
Response to Brigatinib Targeted Therapy in Non-Small Cell Lung Cancer Harboring Epidermal Growth Factor Receptor Exon 19 Deletion, T790M, and cis-C797S Triple Mutations: A Case Report
Authors: Chen Zi-Wei , Lin Gigin , Shih Hsuan-Jen , Wu Chiao-En
Published: December 2022

Epidermal growth factor receptor (EGFR) triple mutations with exon 19 deletion (del19), T790M, and cis-C797S (del19/T790M/cis-C797S mutations) frequently occur in patients with non-small cell lung cancer (NSCLC), while progression to frontline EGFR-tyrosine kinase inhibitors (TKIs) and osimertinib was resistant to all clinically available EGFR-TKIs. Brigatinib monotherapy may be a potential treatment for NSCLC harboring del19/T790M/cis-C797S mutations based on preclinical studies; however, no clinical report has evaluated its efficacy on EGFR del19/T790M/cis-C797S mutations. Herein, we present a case of a female patient with EGFR del19-mutated NSCLC treated with afatinib followed by osimertinib due to acquired T790M mutation. The EGFR del19/T790M/cis-C797S mutations were detected following osimertinib treatment. Complete response of skull metastasis was confirmed after brigatinib treatment (90 mg daily). Unfortunately, she experienced intolerable adverse events; therefore, brigatinib was discontinued after three-month usage. This report provides the first reported evidence for the use of brigatinib monotherapy in patients with NSCLC harboring EGFR del19/T790M/cis-C797S mutations after progression to previous EGFR-TKIs.
Involvement of CXCL17 and GPR35 in Gastric Cancer Initiation and Progression
Authors: Li Yizhi , Liu Aoran , Liu Songyi , Yan Lirong , Yuan Yuan , Xu Qian
Published: December 2022

The expression of CXC motif chemokine 17 (CXCL17) and its reported membrane receptor G-protein-coupled receptor 35 (GPR35) in different gastric pathological lesions and their clinical implications are largely unknown. In this study, a total of 860 pathological sections were immune-stained with either anti-CXCL17 or anti-GPR35 antibodies. Their expression was scored within the area of the normal gastric gland of non-atrophic gastritis (NAG-NOR), intestinal metaplasia of atrophic gastritis (AG-IM), IM adjacent to GC (GC-IM), and GC tissue. The clinical significance and potential function of CXCL17 and GPR35 were explored using multiple methods. Our results suggested that CXCL17 expression was gradually upregulated during the pathological progress of gastric diseases (NAG-NOR < AG-IM < GC-IM), but significantly downregulated when GC occurred. GPR35 had a similar expression pattern but its expression in GC remained abundant. High CXCL17 expression in GC was associated with less malignant behavior and was an independent biomarker of favorable prognosis. Overexpressing CXCL17 in HGC27 cells significantly upregulated CCL20 expression. TCGA analysis identified that CXCL17 was negatively correlated with some cancer-promoting pathways and involved in inflammatory activities. CTRP analysis revealed that gastric cell lines expressing less CXCL17 and were more sensitive to the CXCR2 inhibitor SB-225002.
Induction of ATF4-Regulated Atrogenes Is Uncoupled from Muscle Atrophy during Disuse in Halofuginone-Treated Mice and in Hibernating Brown Bears
Authors: Cussonneau Laura , Coudy-Gandilhon Cécile , Deval Christiane , Chaouki Ghita , Djelloul-Mazouz Mehdi , Delorme Yoann , Hermet Julien , Gauquelin-Koch Guillemette , Polge Cécile , Taillandier Daniel , Averous Julien , Bruhat Alain , Jousse Céline , Papet Isabelle , Bertile Fabrice , Lefai Etienne , Fafournoux Pierre , Maurin Anne-Catherine , Combaret Lydie
Published: December 2022

Activating transcription factor 4 (ATF4) is involved in muscle atrophy through the overexpression of some atrogenes. However, it also controls the transcription of genes involved in muscle homeostasis maintenance. Here, we explored the effect of ATF4 activation by the pharmacological molecule halofuginone during hindlimb suspension (HS)-induced muscle atrophy. Firstly, we reported that periodic activation of ATF4-regulated atrogenes (Gadd45a, Cdkn1a, and Eif4ebp1) by halofuginone was not associated with muscle atrophy in healthy mice. Secondly, halofuginone-treated mice even showed reduced atrophy during HS, although the induction of the ATF4 pathway was identical to that in untreated HS mice. We further showed that halofuginone inhibited transforming growth factor-β (TGF-β) signalling, while promoting bone morphogenetic protein (BMP) signalling in healthy mice and slightly preserved protein synthesis during HS. Finally, ATF4-regulated atrogenes were also induced in the atrophy-resistant muscles of hibernating brown bears, in which we previously also reported concurrent TGF-β inhibition and BMP activation. Overall, we show that ATF4-induced atrogenes can be uncoupled from muscle atrophy. In addition, our data also indicate that halofuginone can control the TGF-β/BMP balance towards muscle mass maintenance. Whether halofuginone-induced BMP signalling can counteract the effect of ATF4-induced atrogenes needs to be further investigated and may open a new avenue to fight muscle atrophy. Finally, our study opens the way for further studies to identify well-tolerated chemical compounds in humans that are able to fine-tune the TGF-β/BMP balance and could be used to preserve muscle mass during catabolic situations.
Molecular Mechanisms Underlying IL-33-Mediated Inflammation in Inflammatory Bowel Disease
Authors: Aggeletopoulou Ioanna , Tsounis Efthymios P. , Triantos Christos
Published: December 2022

Interleukin-33 (IL-33) is a cytokine defined by its pleiotropic function, acting either as a typical extracellular cytokine or as a nuclear transcription factor. IL-33 and its receptor, suppression of tumorigenicity 2 (ST2), interact with both innate and adaptive immunity and are considered critical regulators of inflammatory disorders. The IL-33/ST2 axis is involved in the maintenance of intestinal homeostasis; on the basis of their role as pro- or anti-inflammatory mediators of first-line innate immunity, their expression is of great importance in regard to mucosal defenses. Mucosal immunity commonly presents an imbalance in inflammatory bowel disease (IBD). This review summarizes the main cellular and molecular aspects of IL-33 and ST2, mainly focusing on the current evidence of the pro- and anti-inflammatory effects of the IL-33/ST2 axis in the course of ulcerative colitis and Crohn’s disease, as well as the molecular mechanisms underlying the association of IL-33/ST2 signaling in IBD pathogenesis. Although IL-33 modulates and impacts the development, course, and recurrence of the inflammatory response, the exact role of this molecule is elusive, and it seems to be associated with the subtype of the disease or the disease stage. Unraveling of IL-33/ST2-mediated mechanisms involved in IBD pathology shows great potential for clinical application as therapeutic targets in IBD treatment.
Liraglutide Counteracts Endoplasmic Reticulum Stress in Palmitate-Treated Hypothalamic Neurons without Restoring Mitochondrial Homeostasis
Authors: Griffin Haven , Sullivan Sarah C. , Barger Steven W. , Phelan Kevin D. , Baldini Giulia
Published: December 2022

One feature of high-fat diet-induced neurodegeneration in the hypothalamus is an increased level of palmitate, which is associated with endoplasmic reticulum (ER) stress, loss of CoxIV, mitochondrial fragmentation, and decreased abundance of MC4R. To determine whether antidiabetic drugs protect against ER and/or mitochondrial dysfunction by lipid stress, hypothalamic neurons derived from pre-adult mice and neuronal Neuro2A cells were exposed to elevated palmitate. In the hypothalamic neurons, palmitate exposure increased expression of ER resident proteins, including that of SERCA2, indicating ER stress. Liraglutide reverted such altered ER proteostasis, while metformin only normalized SERCA2 expression. In Neuro2A cells liraglutide, but not metformin, also blunted dilation of the ER induced by palmitate treatment, and enhanced abundance and expression of MC4R at the cell surface. Thus, liraglutide counteracts, more effectively than metformin, altered ER proteostasis, morphology, and folding capacity in neurons exposed to fat. In palmitate-treated hypothalamic neurons, mitochondrial fragmentation took place together with loss of CoxIV and decreased mitochondrial membrane potential (MMP). Metformin, but not liraglutide, reverted mitochondrial fragmentation, and both liraglutide and metformin did not protect against either loss of CoxIV abundance or MMP. Thus, ER recovery from lipid stress can take place in hypothalamic neurons in the absence of recovered mitochondrial homeostasis.
Structural and Functional Characterization of a New Bacterial Dipeptidyl Peptidase III Involved in Fruiting Body Formation in Myxobacteria
Authors: Chen Si-Bo , Zhang Han , Chen Si , Ye Xian-Feng , Li Zhou-Kun , Liu Wei-Dong , Cui Zhong-Li , Huang Yan
Published: December 2022

Dipeptidyl peptidase III (DPP III) is a zinc-dependent enzyme that specifically hydrolyzes dipeptides from the N-terminal of different-length peptides, and it is involved in a number of physiological processes. Here, DPP III with an atypical pentapeptide zinc binding motif (HELMH) was identified from Corallococcus sp. EGB. It was shown that the activity of recombined CoDPP III was optimal at 50 °C and pH 7.0 with high thermostability up to 60 °C. Unique to CoDPP III, the crystal structure of the ligand-free enzyme was determined as a dimeric and closed form. The relatively small inter-domain cleft creates a narrower entrance to the substrate binding site and the unfavorable binding of the bulky naphthalene ring. The ectopic expression of CoDPP III in M. xanthus DK1622 resulted in a 12 h head start in fruiting body development compared with the wild type. Additionally, the A-signal prepared from the starving DK1622-CoDPP III rescued the developmental defect of the asgA mutant, and the fruiting bodies were more numerous and closely packed. Our data suggested that CoDPP III played a role in the fruiting body development of myxobacteria through the accumulation of peptides and amino acids to act as the A-signal.
The Efficacy of Molecular Analysis in the Diagnosis of Bone and Soft Tissue Sarcoma: A 15-Year Mono-Institutional Study
Authors: Benini Stefania , Gamberi Gabriella , Cocchi Stefania , Magagnoli Giovanna , Fortunato Angela Rosa , Sciulli Enrica , Righi Alberto , Gambarotti Marco
Published: December 2022

The histological diagnosis of sarcoma can be difficult as it sometimes requires the combination of morphological and immunophenotypic analyses with molecular tests. A total of 2705 tissue samples of sarcoma consecutively collected from 2006 until 2020 that had undergone molecular analysis were assessed to evaluate their diagnostic utility compared with histological assessments. A total of 3051 molecular analyses were performed, including 1484 gene fusions tested by c/qRT–PCR, 992 gene rearrangements analysed by FISH, 433 analyses of the gene status of MDM2, 126 mutational analyses and 16 NGS analysis. Of the samples analysed, 68% were from formalin-fixed, paraffin-embedded tissue and 32% were from frozen tissue. C/qRT–PCR and FISH analyses were conclusive on formalin-fixed, paraffin-embedded tissue in 74% and 76% of samples, respectively, but the combination of the two methods gave us conclusive results in 96% and 89% of frozen and formalin-fixed, paraffin-embedded tissues, respectively. We demonstrate the utility of c/qRT–PCR and FISH for sarcoma diagnosis and that each has advantages in specific contexts. We conclude that it is possible to accurately predict the sarcoma subtype using a panel of different subtype-specific FISH probes and c/qRT–PCR assays, thereby greatly facilitating the differential diagnosis of these tumours.
Polypropylene and Graphene Nanocomposites: Effects of Selected 2D-Nanofiller’s Plate Sizes on Fundamental Physicochemical Properties
Authors: Patra Sarat Chandra , Swain Sumit , Senapati Pragyan , Sahu Himadri , Murmu Rabiranjan , Sutar Harekrushna
Published: December 2022

The authors developed a nanocomposite using polypropylene (PP) and graphene nanoplatelets (GNPs) with a melt mixing method. Virgin PP was filled with three sets of GNPs with a fixed thickness (15 nm) and surface area (50–80 m2/g). The selected H-type GNPs had three different sizes of 5, 15 and 25 µm. The nanocomposites were made by loading GNPs at 1, 2 and 3 wt.%. Mechanical analysis was carried out by performing tensile, flexural and impact strength tests. The crystalline, micro-structural, thermal and dynamic mechanical properties were assessed through XRD, FESEM, PLM, DSC, TGA and DMA tests. It was observed that all three types of GNPs boosted the mechanical strength of the polymer composite. Increasing the nanofiller size decreased the tensile strength and the tensile modulus, increased the flexural strength and flexural modulus, and increased the impact strength. Maximum tensile strength (≈41.18 MPa) resulted for the composite consisting 3 wt.% H5, whereas maximum flexural (≈50.931 MPa) and impact (≈42.88 J/m) strengths were observed for nanocomposite holding 3 wt.% H25. Graphene induced the PP’s crystalline phases and structure. An improvement in thermal stability was seen based on the results of onset degradation (TD) and melting (Tm) temperatures. Graphene increased the crystallization (Tc) temperatures, and acted like a nucleating agent. The experimental analysis indicated that the lateral size of graphene plays an important role for the nanocomposite’s homogeneity. It was noted that the small-sized GNPs improved dispersion and decreased agglomeration. Thus overall, small-sized GNPs are preferable, and increasing the lateral size hardly establishes feasible characteristics in the nanocomposite.
Evaluation of Interference Analysis from 5G NR Networks to Aeronautical and Maritime Mobile Systems in the Frequency Band 4800–4990 MHz
Authors: Pastukh Alexander , Sorokin Vladislav
Published: December 2022

The current deployment of 5G NR in the frequency band 4800–4990 MHz in multiple countries near the coastlines indicated that there might be a potential risk of harmful interference impacting aeronautical and maritime systems located in international airspace and international waters. This raised numerous concerns about whether 5G NR system rollouts in that band need to be restricted by the power flux density limits created at the border of the territorial waters, which equals 12 nautical miles from the coastline. This work contains a case study based on the example of the Pacific region, where a simulation of aggregate interference from 5G NR base stations and user equipment deployed in the cities near the coastline using Monte Carlo analysis was conducted. The results of the study show that no harmful interference to the aeronautical and maritime services operating in international airspace and waters is expected even when the aircraft or vessels are located at the border of the territorial airspace and waters from the coastline. Therefore, no restriction to the 5G NR deployment in the coastline regions in the frequency band 4800–4990 MHz is required.
Ceramic Crowns and Sleep Bruxism: First Results from a Randomized Trial
Authors: Schmitter Marc , Bömicke Wolfgang , Behnisch Rouven , Lorenzo Bermejo Justo , Waldecker Moritz , Rammelsberg Peter , Ohlmann Brigitte
Published: December 2022

Background: This randomized clinical trial was conducted to assess whether sleep bruxism (SB) is associated with an increased rate of technical complications (ceramic defects) in lithium disilicate (LiDi) or zirconia (Z) molar single crowns (SCs). Methods: Adult patients were classified as affected or unaffected by SB based on structured questionnaires, clinical signs, and overnight portable electromyography (BruxOff) and block randomized into four groups according to SB status and crown material (LiDi or Z): LiDi-SB (n = 29), LiDi-no SB (n = 24), Z-SB (n = 23), and Z-no SB (n = 27). Differences in technical complications (main outcome) and survival and success rates (secondary outcomes) one year after crown cementation were assessed using Fisher’s exact test with significance level α = 0.05. Results: No technical complications occurred. Restoration survival rates were 100% in the LiDi-SB and LiDi-no SB groups, 95.7% in the Z-SB group, and 96.3% in the Z-no SB group (p > 0.999). Success rates were 96.6% in the LiDi-SB group, 95.8% in the LiDi-no SB group (p > 0.999), 91.3% in the Z-SB group, and 96.3% in the Z-no SB group (p ≥ 0.588). Conclusions: With a limited observation time and sample size, no effect of SB on technical complication, survival, and success rates of molar LiDi and Z SCs was detected.
In Search of an Imaging Classification of Adenomyosis: A Role for Elastography?
Authors: Guo Sun-Wei , Benagiano Giuseppe , Bazot Marc
Published: December 2022

Adenomyosis is a complex and poorly understood gynecological disease. It used to be diagnosed exclusively by histology after hysterectomy; today its diagnosis is carried out increasingly by imaging techniques, including transvaginal ultrasound (TVUS) and magnetic resonance imaging (MRI). However, the lack of a consensus on a classification system hampers relating imaging findings with disease severity or with the histopathological features of the disease, making it difficult to properly inform patients and clinicians regarding prognosis and appropriate management, as well as to compare different studies. Capitalizing on our grasp of key features of lesional natural history, here we propose adding elastographic findings into a new imaging classification of adenomyosis, incorporating affected area, pattern, the stiffest value of adenomyotic lesions as well as the neighboring tissues, and other pathologies. We argue that the tissue stiffness as measured by elastography, which has a wider dynamic detection range, quantitates a fundamental biologic property that directs cell function and fate in tissues, and correlates with the extent of lesional fibrosis, a proxy for lesional “age” known to correlate with vascularity and hormonal receptor activity. With this new addition, we believe that the resulting classification system could better inform patients and clinicians regarding prognosis and the most appropriate treatment modality, thus filling a void.
Acute Pancreatitis Severity Prediction: It Is Time to Use Artificial Intelligence
Authors: Tarján Dorottya , Hegyi Péter
Published: December 2022

The clinical course of acute pancreatitis (AP) can be variable depending on the severity of the disease, and it is crucial to predict the probability of organ failure to initiate early adequate treatment and management. Therefore, possible high-risk patients should be admitted to a high-dependence unit. For risk assessment, we have three options: (1) There are univariate biochemical markers for predicting severe AP. One of their main characteristics is that the absence or excess of these factors affects the outcome of AP in a dose-dependent manner. Unfortunately, all of these parameters have low accuracy; therefore, they cannot be used in clinical settings. (2) Score systems have been developed to prognosticate severity by using 4–25 factors. They usually require multiple parameters that are not measured on a daily basis, and they often require more than 24 h for completion, resulting in the loss of valuable time. However, these scores can foresee specific organ failure or severity, but they only use dichotomous parameters, resulting in information loss. Therefore, their use in clinical settings is limited. (3) Artificial intelligence can detect the complex nonlinear relationships between multiple biochemical parameters and disease outcomes. We have recently developed the very first easy-to-use tool, EASY-APP, which uses multiple continuous variables that are available at the time of admission. The web-based application does not require all of the parameters for prediction, allowing early and easy use on admission. In the future, prognostic scores should be developed with the help of artificial intelligence to avoid information loss and to provide a more individualized risk assessment.
Joint Replacement Surgeries Can Be Safely Performed in HIV Patients
Authors: Zhang Chaofan , Li Yongbin , Lin Yunzhi , Zhang Xuehui , Huang Zida , Bai Guochang , Wan Yao , Zhang Wenming , Fang Xinyu , Li Wenbo
Published: December 2022

Whether joint replacement surgery can be performed safely on HIV patients is still a matter of debate. This study aimed to report the surgical efficacy and complications of joint replacement surgery in HIV patients. A total of 21 HIV patients and 27 non-HIV patients who underwent arthroplasties in our hospital were retrospectively reviewed. The 21 HIV patients received 29 joint replacement surgeries including 27 cases of total hip arthroplasty (THA) and 2 cases of total knee arthroplasty (TKA). The non-HIV patients received 16 THA, 10 TKA, and 3 unicompartmental arthroplasty (UKA). The length of hospital stay of HIV patients was significantly lower than that of non-HIV patients. At the last follow-up, there were no significant complications both in the HIV group and the non-HIV groups. No medical staff had any occupational exposure. We concluded that joint replacement surgery in HIV patients is safe and effective. Optimization of patients is key to treatment success. Strictly following the standardized protection protocol can prevent the risk of occupational exposure.
The Periodic Replacement of Adhesive Setae in Pad Lamellae of Climbing Lizards Is Driven by Patterns of Corneous Layer Growth
Authors: Alibardi Lorenzo
Published: December 2022

The adhesive digital pads in some gecko and anoline lizards are continuously utilized for movements on vertical surfaces that may determine wear and a decrease of adhesion efficiency. The pads are formed by lamellae bearing adhesive setae that are worn out following frequent usage and are replaced by new inner setae that maintain an efficient adhesion. Whether the extensive usage of adhesive setae determines a higher shedding frequency in the digital pads with respect to other body regions remains unknown. Setae replacement has been analyzed in embryos and adult lizards using autoradiography and 5BrdU-immunohistochemistry. The observation strongly suggests that during development and epidermal renewal in adult lamellae, there is a shifting of the outer setae toward the apex of the lamella. This movement is likely derived from the continuous addition of proteins in the beta- and alpha-layers sustaining the outer setae while the inner setae are forming. Ultrastructural and in situ hybridization studies indicate that the thin outer beta- and alpha-layers still contain mRNAs and ribosomes that may contribute to the continuous production of corneous beta proteins (CBPs) and keratins for the growth of the free margin at the apex of the lamella. This process determines the apical shifting and release of the old setae, while the new inner setae formed underneath becomes the new outer setae.
Effective Chlorella vulgaris Biomass Harvesting through Sulfate and Chloride Flocculants
Authors: Ma Wei , Feng Chenchen , Guan Fachun , Ma Dianrong , Cai Jinling
Published: December 2022

Efficient microalgae harvesting is a great challenge hindering diverse industrial applications of microalgae. Flocculation is regarded as an effective and promising technology for microalgae harvesting. In this study, sulfate (Al2(SO4)3 and Fe2(SO4)3) and chloride flocculants (AlCl3 and FeCl3) were used to harvest Chlorella vulgaris. Flocculation conditions, including flocculant dose, flocculation time, stirring speed, stirring time, and flocculation pH, were optimized, and flocculant effects on microalgal cell status, floc characteristics, biomass composition, algal cell re-culture, and media recycling were investigated. All flocculants exhibited efficient flocculation efficiency (93.5–98.8%) with lower doses of sulfate salts (60 mg/L algal culture) and higher doses of chloride salts (100 mg/L algal culture). The tested flocculants had no obvious influence on biomass composition (including lipids, carbohydrates, proteins, and carotenoids), and microalgal cells in flocs could efficiently regrow. The spent medium of all treatments was successfully recycled for subsequent cell growth, thus reducing dependency on fresh medium.