bluuebunny/crossref_metadata_2025 huggingface dataset:
The Application of Cameras in Precision Pig Farming: An Overview for Swine-Keeping Professionals
Authors: Arulmozhi Elanchezhian , Bhujel Anil , Moon Byeong-Eun , Kim Hyeon-Tae
Published: August 2021

Pork is the meat with the second-largest overall consumption, and chicken, pork, and beef together account for 92% of global meat production. Therefore, it is necessary to adopt more progressive methodologies such as precision livestock farming (PLF) rather than conventional methods to improve production. In recent years, image-based studies have become an efficient solution in various fields such as navigation for unmanned vehicles, human–machine-based systems, agricultural surveying, livestock, etc. So far, several studies have been conducted to identify, track, and classify the behaviors of pigs and achieve early detection of disease, using 2D/3D cameras. This review describes the state of the art in 3D imaging systems (i.e., depth sensors and time-of-flight cameras), along with 2D cameras, for effectively identifying pig behaviors and presents automated approaches for the monitoring and investigation of pigs’ feeding, drinking, lying, locomotion, aggressive, and reproductive behaviors.
Adaptation Mechanisms of Yak (Bos grunniens) to High-Altitude Environmental Stress
Authors: Ayalew Wondossen , Chu Min , Liang Chunnian , Wu Xiaoyun , Yan Ping
Published: August 2021

Living at a high altitude involves many environmental challenges. The combined effects of hypoxia and cold stress impose severe physiological challenges on endothermic animals. The yak is integral to the livelihood of the people occupying the vast, inhospitable Qinghai–Tibetan plateau and the surrounding mountainous region. Due to long-term selection, the yak exhibits stable and unique genetic characteristics which enable physiological, biochemical, and morphological adaptations to a high altitude. Thus, the yak is a representative model for mammalian plateau-adaptability studies. Understanding coping mechanisms provides unique insights into adaptive evolution, thus informing the breeding of domestic yaks. This review provides an overview of genetic adaptations in Bos grunniens to high-altitude environmental stress. Combined genomics and theoretical advances have informed the genetic basis of high-altitude adaptations.
A Comparison of Key Essential Nutrients in Commercial Plant-Based Pet Foods Sold in Canada to American and European Canine and Feline Dietary Recommendations
Authors: Dodd Sarah A. S. , Shoveller Anna K. , Fascetti Andrea J. , Yu Zengshou Z. , Ma David W. L. , Verbrugghe Adronie
Published: August 2021

Plant-based foods intended for feeding dogs and cats are available in Canada, though few studies have examined the suitability of plant-based foods for dogs and cats. All commercial plant-based extruded and wet pet food products available in Ontario, Canada, in 2018 (n = 26) were acquired and analysed for energy, crude protein, crude fat, crude fibre, ash, amino acids, fatty acids, minerals and vitamins A, B12, D2 and D3. Results were compared with recommendations of the Association of American Feed Control Officials (AAFCO) and the European Pet Food Industry Federation (FEDIAF). Thirteen products were labelled for adult canine maintenance, four for canine all life stages, one for puppy growth, two for adult feline maintenance, three for feline all life stages, one for adult maintenance of dogs and cats and two for all life stages of dogs and cats. Four products met AAFCO and one product met FEDIAF nutrient recommendations for canine maintenance. No diets met AAFCO or FEDIAF recommendations for feline maintenance or growth for either species. Nutrients most commonly found insufficient were: sulfur amino acids, taurine, arachidonic acid, EPA and DHA, calcium phosphorus and vitamin D. There were no nutrients unable to be provided from non-animal sources. Compliance with labelling guidelines was also poor, similar to other findings with commercial animal-based pet products. The results from this study indicate areas where producers of plant-based pet foods must improve to meet the industry recommended nutrient profiles and labelling requirements.
Comparison of Antioxidant Properties of a Conjugate of Taxifolin with Glyoxylic Acid and Selected Flavonoids
Authors: Shubina Victoria S. , Kozina Victoria I. , Shatalin Yuri V.
Published: August 2021

It is known that flavonoids can react with toxic carbonyl compounds in the process of the storage, aging, and digestion of flavonoid-rich foods and beverages. However, the effect of these reactions on the antioxidant properties of the polyphenolic fraction and the properties of the resulting products remain poorly studied. The aim of the present work was to study the antioxidant activity of quercetin, taxifolin, catechin, eriodictyol, hesperetin, naringenin and a product of the condensation of taxifolin with glyoxylic acid, as well as to reveal the structure–activity relationship of these polyphenols. It was found that flavonoids containing the catechol moiety exhibited higher antioxidant activity than hesperetin and naringenin. The product showed the highest hydrogen peroxide scavenging activity, a lower metal-reducing and a higher iron-binding ability than catechol-containing flavonoids, and a lipid peroxidation inhibitory activity comparable with that of taxifolin. Thus, the condensation of flavonoids with toxic carbonyl compounds might lead to the formation of products exhibiting high antioxidant activity. Meanwhile, the conditions under which parent flavonoids and their products exhibit the maximal antioxidant activity may differ. The data suggest that the antioxidant profile of the polyphenolic fraction and bioavailability of polyphenols, carbonyl compounds, and metal ions may change when these reactions occur.
Oxidative Stress and Antioxidant Pathway in Allergic Rhinitis
Authors: Han Munsoo , Lee Dabin , Lee Sang Hag , Kim Tae Hoon
Published: August 2021

Oxidative stress is the cause and consequence of redox metabolism in various physiological and pathological conditions. Understanding the molecular pathways underlying oxidative stress and the role of antioxidants could serve as the key to helping treat associated diseases. Allergic rhinitis is a condition that deteriorates the daily function and quality of life of afflicted individuals and is associated with a high socioeconomic burden and prevalence. Recent studies have focused on the role of oxidative stress and antioxidants in allergic rhinitis. This review discusses animal and clinical studies on oxidative markers and the potential therapeutic dietary antioxidants for allergic rhinitis.
Reducing Soil Permeability Using Bacteria-Produced Biopolymer
Authors: Mendonça Amanda , Morais Paula V. , Pires Ana Cecília , Chung Ana Paula , Oliveira Paulo J. Venda
Published: August 2021

The building of civil engineering structures on some soils requires their stabilisation. Although Portland cement is the most used substance to stabilise soils, it is associated with a lot of environmental concerns. Therefore, it is very pertinent to study more sustainable alternative methodologies to replace the use of cement. Thus, this work analyses the ability of the more sustainable xanthan-like biopolymer, produced by Stenotrophomonas maltophilia Faro439 strain (LabXLG), to reduce the permeability of a sandy soil. Additionally, the effectiveness of this LabXLG is compared with the use of a commercial xanthan gum (XG) and cement for various hydraulic gradients and curing times. The results show that a treatment with either type of XG can be used to replace the cement over the short term (curing time less than 14 days), although a greater level of effectiveness is obtained with the use of the commercial XG, due to its higher level of purity. The soil treatment with LabXLG creates a network of fibres that link the soil particles, while the commercial XG fills the voids with a homogeneous paste.
Are We Able to Match Non Sport-Specific Strength Training with Endurance Sports? A Systematic Review and Meta-Analysis to Plan the Best Training Programs for Endurance Athletes
Authors: Ambrosini Luca , Presta Valentina , Goldoni Matteo , Galli Daniela , Mirandola Prisco , Vitale Marco , Gobbi Giuliana
Published: August 2021

Non-sport-specific strength training is a way to increase endurance performance; however, which kind of exercise (maximal, plyometric, explosive or resistance strength training) gives the best results is still under debate. Scientific publications were analyzed according to the PRISMA checklist and statement. The initial search yielded 500 studies, 17 of which were included in this review using the PEDro Scale. Maximal strength training boosted the ability to express strength particularly in cross-country skiing and cycling, increasing endurance performance, measured as a decrease of the endurance performance tests. In running, explosive strength training did not generate advantages, whereas plyometric strength training led to an improvement in the endurance performance tests and work economy. In running it was possible to compare different types of non sport-specific strength training and the plyometric one resulted the best training methodology to enhance performance. However, studies on other sports only investigated the effects of maximal strength training. It resulted more effective in cross-country skiing (although only one study was eligible according to the inclusion criteria) and in the cycling component of the triathlon and, by contrast, induced modest effects on cyclists’ performance, suggesting different type of strength would probably be more effective. In conclusion, each sport might optimize performance by using appropriate non sport-specific strength training, which, however, should be studied individually.
Effect of Freeze–Thaw Cycles on the Performance of Concrete Containing Water-Cooled and Air-Cooled Slag
Authors: Lee Seung-Tae , Park Se-Ho , Kim Dong-Gyou , Kang Jae-Mo
Published: August 2021

An experimental study on the resistance of concrete containing air-cooled slag (AS) and water-cooled slag (WS) against freeze–thaw cycles was conducted. For comparison, the durability of ASTM Type I ordinary Portland cement (OPC) concrete exposed to the same freeze–thaw environment was examined. To evaluate the durability of concrete exposed to the freeze–thaw environment, an experiment was conducted according to ASTM C 666 procedure A. Furthermore, the relative dynamic modulus of elasticity, surface electrical resistivity, and compressive strength of concrete specimens were measured after exposing them to freeze–thaw cycles for a predetermined period, and the results were compared with those of OPC concrete. The relationship between the freeze and thaw resistances of concrete and the air-void system (spacing factor and specific surface area) was identified. Furthermore, the microstructure of concrete exposed to freeze–thaw cycles was observed using scanning electron microscopy to identify the interfacial transition zone, cracks, and micropores. Experimental results showed that the resistance of blended cement concrete containing WS and AS against freeze–thaw cycles was significantly higher than that of OPC concrete. The concrete in which 10% of OPC was replaced by AS exhibited a similar durability as that of the concrete in which 40% of OPC was replaced only by WS. Therefore, it is expected that blended cement concrete containing WS and AS based on an appropriate mix proportion design will exhibit excellent durability in regions experiencing freezing temperatures.
A Novel Sol-Gel Bi2-xHfxO3+x/2 Radiopacifier for Mineral Trioxide Aggregates (MTA) as Dental Filling Materials
Authors: Yang Tzu-Sen , Chen May-Show , Huang Cheng-Jyun , Chen Chin-Yi , Brangule Agnese , Zarkov Aleksej , Kareiva Aivaras , Lin Chung-Kwei , Yang Jen-Chang
Published: August 2021

Mineral trioxide aggregate (MTA) is well known as an effective root canal filling material for endodontics therapy. Within MTA, bismuth oxide (Bi2O3) serving as the radiopacifier still has biocompatibility concerns due to its mild cytotoxicity. In the present study, we tried to modify the Bi2O3 radiopacifier by doping hafnium ions via the sol-gel process and investigated the effects of different doping ratios (Bi2-xHfxO3+x/2, x = 0–0.3) and calcination temperatures (400–800 °C). We mixed various precursor mixtures of bismuth nitrate (Bi(NO3)3·5H2O) and hafnium sulfate (Hf(SO4)2) and controlled the calcination temperatures. The as-prepared Hf-doped Bi2O3 radiopacifier powders were investigated by thermogravimetric analysis (TGA), X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). Portland cement/radiopacifier/calcium sulfate (75/20/5) were mixed and set by deionized water (powder to water ratio = 3:1). Changes in radiopacity, diametral tensile strength (DTS), and in vitro cell viability of the hydrated MTA-like cement were carried out. The experimental results showed that the group containing radiopacifier from sol-gelled Bi/Hf (90/10) exhibited significantly higher radiopacity (6.36 ± 0.34 mmAl), DTS (2.54 ± 0.29 MPa), and cell viability (84.0±8.1%) (p < 0.05) when compared to that of Bi/Hf (100/0) powders. It is suggested that the formation of β-Bi7.78Hf0.22O12.11 phase with hafnium addition and calcining at 700 °C can prepare novel bismuth/hafnium composite powder that can be used as an alternative radiopacifier for root canal filling materials.
The Effect of the Selection of Three-Dimensional Random Numerical Soil Models on Strip Foundation Settlements
Authors: Żyliński Kamil , Winkelmann Karol , Górski Jarosław
Published: August 2021

This paper delivers a probabilistic attempt to prove that the selection of a random three-dimensional finite element (FE) model of a subsoil affects the computed settlements. Parametric analysis of a random soil block is conducted, assuming a variable subsoil Young’s modulus in particular finite elements. The modulus is represented by a random field or different-sized sets of random variables; in both cases, the same truncated Gaussian model is assumed. Mean values and standard deviations of random soil settlement are estimated by a Monte Carlo simulation procedure. With regard to the adopted FE model, the estimated settlement mean values do not vary significantly, but standard deviations do strongly. Similarities also appear in the diagrams of random field correlation length versus settlement standard deviation and the diagrams displaying a total number of model random variables versus settlement standard deviation. Thus, relevant single random variable models represent the random field approach well with regard to settlement parameter estimation. This remark is verified upon a settlement analysis of a three-dimensional FE model of a hypothetical strip foundation. Following the preliminary model observations, various probabilistic geotechnical analyses may be supported, e.g., continuous footing design, slope stability analysis, and foundation reliability assessment.
Development of Multifunctional Myoelectric Hand Prosthesis System with Easy and Effective Mode Change Control Method Based on the Thumb Position and State
Authors: Jung Sung-Yoon , Kim Seung-Gi , Kim Joo-Hyung , Park Se-Hoon
Published: August 2021

Commercial multi-degrees-of-freedom (multi-DOF) myoelectric hand prostheses can perform various hand gestures and grip motions using multiple DOFs. However, as most upper limb amputees have less than two electromyogram (EMG) signals generated at the amputation site, it is difficult to control various hand gestures and grip motions using multi-DOF myoelectric hand prostheses. This paper proposes a multifunctional myoelectric hand prosthesis system that uses only two EMG sensors while improving the convenience of upper limb amputees in everyday life. The proposed system comprises a six-DOF myoelectric hand prosthesis and an easy and effective control algorithm that enables upper limb amputees to perform various hand gestures and grip motions. More specifically, the hand prosthesis has a multi-DOF five-finger mechanism and a small controller that can be mounted inside the hand, allowing it to perform various hand gestures and grip motions. The control algorithm facilitates four grip motions and four gesture motions using the adduction and abduction positions of the thumb, the flexion and extension state of the thumb, and three EMG signals (co-contraction, flexion, and extension) generated using the two EMG sensors. Experimental results indicate that the proposed system is a versatile, flexible, and effective hand prosthesis system for upper limb amputees.
Ossification of the Anterior Longitudinal Ligament with Dysphagia as the First Symptom: Rehabilitation of Two Cases
Authors: Nishida Norihiro , Ogasa Hiroyoshi , Seki Kazushige , Kato Tomohiro , Imajo Yasuaki , Suzuki Hidenori , Funaba Masahiro , Yukata Kiminori , Sakai Takashi
Published: August 2021

Dysphagia is associated with poor quality of life, and pneumonia due to aspiration is life-threatening. Cervical ossification of the anterior longitudinal ligament (C-OALL) is one of the causes of dysphagia, and we report two cases in which dysphagia improved after surgery. Case 1: A 76-year-old man had C-OALL of greater than 16 mm and dysphagia and developed myelopathy. A fall resulted in upper and lower limb insufficiency paralysis, and posterior decompression fixation was performed. Pressure on the pharynx by C-OALL remained, but dysphagia improved. Improvement in this case was considered to be due to the loss of intervertebral mobility. Case 2: A 62-year-old man developed dysphagia 6 years ago. It gradually exacerbated, and the C-OALL increased. Laryngeal fiberscope and swallowing angiography revealed that the pharyngeal cavity was compressed and narrowed anteriorly due to ossification. Resection of the ossification was performed, and the patient’s symptoms improved. Direct decompression was successful in this case. Several evaluation methods for dysphagia have been reported, including screening tests, endoscopy, contrast studies, and radiological evaluation. In case 1, extensive ossification was improved by posterior fixation, albeit incidentally, whereas in case 2, a patient with extensive ossification exhibited symptoms. It is necessary to examine the cervical mobility, extent and morphology of ossification, and timing of surgery stenosis to determine the risk factors and treatment options, including rehabilitation.
Modeling a Pre-Touch Reaction Distance around Socially Touchable Upper Body Parts of a Robot
Authors: Cuello Mejía Dario Alfonso , Sumioka Hidenobu , Ishiguro Hiroshi , Shiomi Masahiro
Published: August 2021

Although before-touch situations are essential to achieve natural touch interactions between people and robots, they receive less attention than after-touch situations. This study reports pre-touch reaction distance analysis results around touchable upper body parts, i.e., shoulders, elbows, and hands, based on human–human pre-touch interaction. We also analyzed the effects of gender, approach side, speed, and acclimation in modeling the pre-touch reaction distance, and found that the distance around the hands is smaller than the distance around the shoulders and elbows, and speed and acclimation affect the distance. On the other hand, gender and approach side do not significantly affect the pre-touch reaction distance. Finally, we implemented the results in a male-looking android and confirmed that it reacted toward pre-touch based on the obtained model.
Multilateration Approach for Wide Range Visible Light Indoor Positioning System Using Mobile CMOS Image Sensor
Authors: Rahman Md Habibur , Sejan Mohammad Abrar Shakil , Chung Wan-Young
Published: August 2021

Visible light positioning (VLP) is a cost-effective solution to the increasing demand for real-time indoor positioning. However, owing to high computational costs and complicated image processing procedures, most of the existing VLP systems fail to deliver real-time positioning ability and better accuracy for image sensor-based large-area indoor environments. In this study, an effective method is proposed to receive coordinate information from multiple light-emitting diode (LED) lights simultaneously. It provides better accuracy in large experimental areas with many LEDs by using a smartphone-embedded image sensor as a terminal device and the existing LED lighting infrastructure. A flicker-free frequency shift on–off keying line coding modulation scheme was designed for the positioning system to ensure a constant modulated frequency. We tested the performance of the decoding accuracy with respect to vertical and horizontal distance, which utilizes a rolling shutter mechanism of a complementary metal-oxide-semiconductor image sensor. The experimental results of the proposed positioning system can provide centimeter-level accuracy with low computational time, rendering it a promising solution for the future direction of large-area indoor positioning systems.
Volatile Organic Compounds of the Glandular Trichomes of Ocimum basilicum and Artifacts during the Distillation of the Leaves
Authors: Tirillini Bruno , Maggi Filippo
Published: August 2021

Focusing on volatile organic compounds (VOC) of Ocimum basilicum, this study aims to determine the chemical composition of VOC in secretory trichomes and compare it with that of essential oil obtained by hydrodistillation of leaves. The technique of extracting the content of glandular trichomes refers to the microneedle shuttle analysis. Hydrodistillation of fresh leaves was done with a Clevenger distiller (EO). The chemical compositions were determined by GC/FID and GC/MS. The head of the capitate trichomes does not contain volatile compounds. Fifty volatile compounds were detected in the EO, and twenty-four volatile compounds were detected in the VOC; the main components were eugenol (from 15.47 ± 1.05% to 41.89 ± 2.83%) and linalool (from 32.05 ± 2.57% to 28.99 ± 2.32%), respectively. During the distillation of the basil leaves 26 artifacts are formed. The composition of the essential oil of O. basilicum therefore depends not only on the plant but also on the method used to obtain it.
An Intelligent Error Correction Algorithm for Elderly Care Robots
Authors: Zhang Xin , Feng Zhiquan , Yang Xiaohui , Xu Tao , Qiu Xiaoyu , Hou Ya
Published: August 2021

With the development of deep learning, gesture recognition systems based on the neural network have become quite advanced, but the application effect in the elderly is not ideal. Due to the change of the palm shape of the elderly, the gesture recognition rate of most elderly people is only about 70%. Therefore, in this paper, an intelligent gesture error correction algorithm based on game rules is proposed on the basis of the AlexNet. Firstly, this paper studies the differences between the palms of the elderly and young people. It also analyzes the misread gesture by using the probability statistics method and establishes a misread-gesture database. Then, based on the misreading-gesture library, the maximum channel number of different gestures in the fifth layer is studied by using the similar curve algorithm and the Pearson algorithm. Finally, error correction is completed under the game rule. The experimental results show that the gesture recognition rate of the elderly can be improved to more than 90% by using the proposed intelligent error correction algorithm. The elderly-accompanying robot can understand people’s intentions more accurately, which is well received by users.
A Novel Anatomy Education Method Using a Spatial Reality Display Capable of Stereoscopic Imaging with the Naked Eye
Authors: Itamiya Tomoki , To Masahiro , Oguchi Takeshi , Fuchida Shinya , Matsuo Masato , Hasegawa Iwao , Kawana Hiromasa , Kimoto Katsuhiko
Published: August 2021

Several efforts have been made to use virtual reality (VR) and augmented reality (AR) for medical and dental education and surgical support. The current methods still require users to wear devices such as a head-mounted display (HMD) and smart glasses, which pose challenges in hygiene management and long-term use. Additionally, it is necessary to measure the user’s inter-pupillary distance and to reflect it in the device settings each time to accurately display 3D images. This setting is difficult for daily use. We developed and implemented a novel anatomy education method using a spatial reality display capable of stereoscopic viewing with the naked eye without an HMD or smart glasses. In this study, we developed two new applications: (1) a head and neck anatomy education application, which can display 3D-CG models of the skeleton and blood vessels of the head and neck region using 3D human body data available free of charge from public research institutes, and (2) a DICOM image autostereoscopic viewer, which can automatically convert 2D CT/MRI/CBCT image data into 3D-CG models. In total, 104 students at the School of Dentistry experienced and evaluated the system, and the results suggest its usefulness. A stereoscopic display without a head-mounted display is highly useful and promising for anatomy education.
Purification and In Vitro Evaluation of an Anti-HER2 Affibody-Monomethyl Auristatin E Conjugate in HER2-Positive Cancer Cells
Authors: Damiani Isabella , Castiglioni Silvia , Sochaj-Gregorczyk Alicja , Bonacina Fabrizia , Colombo Irma , Rusconi Valentina , Otlewski Jacek , Corsini Alberto , Bellosta Stefano
Published: August 2021

A promising approach for the development of high-affinity tumor targeting ADCs is the use of engineered protein drugs, such as affibody molecules, which represent a valuable alternative to monoclonal antibodies (mAbs) in cancer-targeted therapy. We developed a method for a more efficient purification of the ZHER2:2891DCS affibody conjugated with the cytotoxic antimitotic agent auristatin E (MMAE), and its efficacy was tested in vitro on cell viability, proliferation, migration, and apoptosis. The effects of ZHER2:2891DCS-MMAE were compared with the clinically approved monoclonal antibody trastuzumab (Herceptin®). To demonstrate that ZHER2:2891DCS-MMAE can selectively target HER2 overexpressing tumor cells, we used three different cell lines: the human adenocarcinoma cell lines SK-BR-3 and ZR-75-1, both overexpressing HER2, and the triple-negative breast cancer cell line MDA-MB-231. MTT assay showed that ZHER2:2891DCS-MMAE induces a significant time-dependent toxic effect in SK-BR-3 cells. A 30% reduction of cell viability was already found after 10 min exposure at a concentration of 7 nM (IC50 of 80.2 nM). On the contrary, MDA-MB-231 cells, which express basal levels of HER2, were not affected by the conjugate. The cytotoxic effect of the ZHER2:2891DCS-MMAE was confirmed by measuring apoptosis by flow cytometry. In SK-BR-3 cells, increasing concentrations of conjugated affibody induced cell death starting from 10 min of treatment, with the strongest effect observed after 48 h. Overall, these results demonstrate that the ADC, formed by the anti-HER2 affibody conjugated to monomethyl auristatin E, efficiently interacts with high affinity with HER2 positive cancer cells in vitro, allowing the selective and specific delivery of the cytotoxic payload.
Diabetic Foot: The Role of Fasciae, a Narrative Review
Authors: Pirri Carmelo , Fede Caterina , Pirri Nina , Petrelli Lucia , Fan Chenglei , De Caro Raffaele , Stecco Carla
Published: August 2021

Wound healing is an intricate, dynamic process, in which various elements such as hyperglycemia, neuropathy, blood supply, matrix turnover, wound contraction, and the microbiome all have a role in this “out of tune” diabetic complex symphony, particularly noticeable in the complications of diabetic foot. Recently it was demonstrated that the fasciae have a crucial role in proprioception, muscular force transmission, skin vascularization and tropism, and wound healing. Indeed, the fasciae are a dynamic multifaceted meshwork of connective tissue comprised of diverse cells settled down in the extracellular matrix and nervous fibers; each constituent plays a particular role in the fasciae adapting in various ways to the diverse stimuli. This review intends to deepen the discussion on the possible fascial role in diabetic wounds. In diabetes, the thickening of collagen, the fragmentation of elastic fibers, and the changes in glycosaminoglycans, in particular hyaluronan, leads to changes in the stiffness, gliding, and the distribution of force transmission in the fasciae, with cascading repercussions at the cellular and molecular levels, consequently feeding a vicious pathophysiological circle. A clear scientific perception of fascial role from microscopic and macroscopic points of view can facilitate the identification of appropriate treatment strategies for wounds in diabetes and create new perspectives of treatment.
Routes for Potassium Ions across Mitochondrial Membranes: A Biophysical Point of View with Special Focus on the ATP-Sensitive K+ Channel
Authors: Kravenska Yevheniia , Checchetto Vanessa , Szabo Ildiko
Published: August 2021

Potassium ions can cross both the outer and inner mitochondrial membranes by means of multiple routes. A few potassium-permeable ion channels exist in the outer membrane, while in the inner membrane, a multitude of different potassium-selective and potassium-permeable channels mediate K+ uptake into energized mitochondria. In contrast, potassium is exported from the matrix thanks to an H+/K+ exchanger whose molecular identity is still debated. Among the K+ channels of the inner mitochondrial membrane, the most widely studied is the ATP-dependent potassium channel, whose pharmacological activation protects cells against ischemic damage and neuronal injury. In this review, we briefly summarize and compare the different hypotheses regarding the molecular identity of this patho-physiologically relevant channel, taking into account the electrophysiological characteristics of the proposed components. In addition, we discuss the characteristics of the other channels sharing localization to both the plasma membrane and mitochondria.
The Interaction of Hemin, a Porphyrin Derivative, with the Purified Rat Brain 2-Oxoglutarate Carrier
Authors: Miniero Daniela Valeria , Spagnoletta Anna , Gambacorta Nicola , Scalera Vito , Pierri Ciro Leonardo , Nicolotti Orazio , De Palma Annalisa
Published: August 2021

The mitochondrial 2-oxoglutarate carrier (OGC), isolated and purified from rat brain mitochondria, was reconstituted into proteoliposomes to study the interaction with hemin, a porphyrin derivative, which may result from the breakdown of heme-containing proteins and plays a key role in several metabolic pathways. By kinetic approaches, on the basis of the single binding centre gated pore mechanism, we analyzed the effect of hemin on the transport rate of OGC in uptake and efflux experiments in proteoliposomes reconstituted in the presence of the substrate 2-oxoglutarate. Overall, our experimental data fit the hypothesis that hemin operates a competitive inhibition in the 0.5–10 µM concentration range. As a consequence of the OGC inhibition, the malate/aspartate shuttle might be impaired, causing an alteration of mitochondrial function. Hence, considering that the metabolism of porphyrins implies both cytoplasmic and mitochondrial processes, OGC may participate in the regulation of porphyrin derivatives availability and the related metabolic pathways that depend on them (such as oxidative phosphorylation and apoptosis). For the sake of clarity, a simplified model based on induced-fit molecular docking supported the in vitro transport assays findings that hemin was as good as 2-oxoglutarate to bind the carrier by engaging specific ionic hydrogen bond interactions with a number of key residues known for participating in the similarly located mitochondrial carrier substrate binding site.
Role of Epigenetics in Type 2 Diabetes and Obesity
Authors: Capparelli Rosanna , Iannelli Domenico
Published: August 2021

Epigenetic marks the genome by DNA methylation, histone modification or non-coding RNAs. Epigenetic marks instruct cells to respond reversibly to environmental cues and keep the specific gene expression stable throughout life. In this review, we concentrate on DNA methylation, the mechanism often associated with transgenerational persistence and for this reason frequently used in the clinic. A large study that included data from 10,000 blood samples detected 187 methylated sites associated with body mass index (BMI). The same study demonstrates that altered methylation results from obesity (OB). In another study the combined genetic and epigenetic analysis allowed us to understand the mechanism associating hepatic insulin resistance and non-alcoholic disease in Type 2 Diabetes (T2D) patients. The study underlines the therapeutic potential of epigenetic studies. We also account for seemingly contradictory results associated with epigenetics.
Calculation of Fetal Fraction for Non-Invasive Prenatal Testing
Authors: Cserhati Matthew
Published: August 2021

Estimating the fetal fraction of DNA in a pregnant mother’s blood is a risk-free, non-invasive way of predicting fetal aneuploidy. It is a rapidly developing field of study, offering researchers a plethora of different complementary methods. Such methods include examining the differences in methylation profiles between the fetus and the mother. Others include calculating the average allele frequency based on the difference in genotype of a number of single-nucleotide polymorphisms. Differences in the length distribution of DNA fragments between the mother and the fetus as well as measuring the proportion of DNA reads mapping to the Y chromosome also constitute fetal fraction estimation methods. The advantages and disadvantages of each of these main method types are discussed. Moreover, several well-known fetal fraction estimation methods, such as SeqFF, are described and compared with other methods. These methods are amenable to not only the estimation of fetal fraction but also paternity, cancer, and transplantation monitoring studies. NIPT is safe, and should aneuploidy be detected, this information can help parents prepare mentally and emotionally for the birth of a special needs child.
Neuroprotective Potential of Mild Uncoupling in Mitochondria. Pros and Cons
Authors: Zorov Dmitry B. , Andrianova Nadezda V. , Babenko Valentina A. , Pevzner Irina B. , Popkov Vasily A. , Zorov Savva D. , Zorova Ljubava D. , Plotnikov Egor Yu. , Sukhikh Gennady T. , Silachev Denis N.
Published: August 2021

There has been an explosion of interest in the use of uncouplers of oxidative phosphorylation in mitochondria in the treatment of several pathologies, including neurological ones. In this review, we analyzed all the mechanisms associated with mitochondrial uncoupling and the metabolic and signaling cascades triggered by uncouplers. We provide a full set of positive and negative effects that should be taken into account when using uncouplers in experiments and clinical practice.
The Effects of the COVID19-Related Lockdown Are Modulated by Age: An Italian Study in Toddlers and Pre-Schoolers
Authors: Cerasuolo Mariangela , Malloggi Serena , Conte Francesca , Albinni Benedetta , De Rosa Oreste , Rescott Marissa Lynn , Giganti Fiorenza , Ficca Gianluca
Published: August 2021

Although the issue has been repeatedly explored, data on the impact of the COVID-19 pandemic on children’s sleep quality are inconsistent. To clarify these discrepancies, here we investigate possible age-related differences. During the lockdown, 112 parents of toddlers (0–3 years, N = 61) and pre-schoolers (4–5 years, n = 51) completed an online survey including the Children’s Sleep Habits Questionnaire (CSHQ). Sleep-related items required an additional retrospective judgment, referring to the pre-pandemic period. During the lockdown, sleep schedules were delayed in both age groups whereas sleep quality (CSHQ total scores) improved in pre-schoolers but not in toddlers. Between-groups comparisons revealed that, prior to the lockdown, pre-schoolers showed worse sleep quality than toddlers, whereas this difference disappeared during home confinement. Also, pre-schoolers’ sleep timing was advanced before the lockdown and delayed during the lockdown relative to toddlers’. Our data highlight a significant modulation of age on the impact of the pandemic crisis on sleep, with pre-schoolers experiencing greater effects than toddlers. This profile suggests that factors affecting sleep features have different weights at different ages: sleep patterns would be mainly determined by developmental factors (i.e., biological drive) in younger children, whereas environmental factors (e.g., major lifestyle changes) would have a stronger effect on older ones.
Comparison of Wind Tunnel Test Data for Low-Rise Buildings with Main Wind Force Resisting System Design Procedures
Authors: Hoq S. M. Ashfaqul , Judd Johnn P.
Published: August 2021

The adequacy of the directional and envelope procedures for the design of the main wind force resisting system is not well understood. The purpose of this study is to evaluate the directional and envelope procedures based on wind tunnel test data for a set of low-rise enclosed buildings with gable-shaped roofs in open terrain (Exposure C). The base shear force and the conditional reliability index are used to determine the adequacy of the procedures. The base shear was compared to the design base shear in each direction based on the horizontal component of the wind load on the wall and roof. The reliability index, β conditional on the occurrence of the design wind speed was computed for a range of system capacities. The main findings are (1) the directional procedure produced a larger design base shear compared to the envelope procedure, primarily due to the difference in external pressure coefficients, (2) the directional procedure provided a higher β, and (3) the envelope procedure provided a β that did not meet the standard target β equal to 3.0 for the main wind force resisting systems with low variability in capacity, but neither procedure met the standard target β for the main wind force resisting systems with high variability in capacity.
Current Treatment Strategies for Non-Small-Cell Lung Cancer with Comorbid Interstitial Pneumonia
Authors: Ikeda Satoshi , Kato Terufumi , Kenmotsu Hirotsugu , Sekine Akimasa , Baba Tomohisa , Ogura Takashi
Published: August 2021

Of patients with advanced non-small-cell lung cancer (NSCLC), 5–10% have interstitial pneumonia (IP) at the time of diagnosis. To avoid fatal acute exacerbations of pre-existing IP, appropriate patient selection and low-risk treatment choices are warranted. Risk factors for acute exacerbation of pre-existing IP with cytotoxic drugs include honeycomb lungs on computed tomography (CT) and low forced vital capacity, but risk factors with immune checkpoint inhibitors (ICIs) have not been fully investigated. For advanced or recurrent NSCLC with comorbid IP, carboplatin plus nanoparticle albumin-bound paclitaxel is the standard of care for first-line treatment, but second-line or later treatment has not been established. ICI holds great promise for long-term survival, but many challenges remain, including safety and appropriate patient selection. Since the indications for pharmacotherapy and radiotherapy for NSCLC with comorbid IP are quite limited, surgical resection should be considered as much as possible for patients with operable stages. A scoring system has been reported to predict the risk of postoperative acute exacerbation of pre-existing IP, but perioperative treatment has not been established. In the future, it is necessary to accumulate more cases and conduct further research, not only in Japan but also worldwide.
Somatostatin Therapy Improves Stellate Cell Activation and Early Fibrogenesis in a Preclinical Model of Extended Major Hepatectomy
Authors: Hessheimer Amelia J. , Vengohechea Jordi , Martínez de la Maza Lilia , Muñoz Javier , Vendrell Marina , Sanahuja Josep Martí , Torroella Alba , Adel Al Shwely Farah , Riquelme Francisco , Muñoz César , García Rocío , Taurá Pilar , Fondevila Constantino
Published: August 2021

Liver resection treats primary and secondary liver tumors, though clinical applicability is limited by the remnant liver mass and quality. Herein, major hepatic resections were performed in pigs to define changes associated with sufficient and insufficient remnants and improve liver-specific outcomes with somatostatin therapy. Three experimental groups were performed: 75% hepatectomy (75H), 90% hepatectomy (90H), and 90% hepatectomy + somatostatin (90H + SST). Animals were followed for 24 h (N = 6) and 5 d (N = 6). After hepatectomy, portal pressure gradient was higher in 90H versus 75H and 90H + SST (8 (3–13) mmHg vs. 4 (2–6) mmHg and 4 (2–6) mmHg, respectively, p < 0.001). After 24 h, changes were observed in 90H associated with stellate cell activation and collapse of sinusoidal lumen. Collagen chain type 1 alpha 1 mRNA expression was higher, extracellular matrix width less, and percentage of collagen-staining areas greater at 24 h in 90H versus 75H and 90H + SST. After 5 d, remnant liver mass was higher in 75H and 90H + SST versus 90H, and Ki-67 immunostaining was higher in 90H + SST versus 75H and 90H. As well, more TUNEL-staining cells were observed in 90H versus 75H and 90H + SST at 5 d. Perioperative somatostatin modified portal pressure, injury, apoptosis, and stellate cell activation, stemming changes related to hepatic fibrogenesis seen in liver remnants not receiving treatment.
The 3.0 Cell Communication: New Insights in the Usefulness of Tunneling Nanotubes for Glioblastoma Treatment
Authors: Taiarol Lorenzo , Formicola Beatrice , Fagioli Stefano , Sierri Giulia , D’Aloia Alessia , Kravicz Marcelo , Renda Antonio , Viale Francesca , Dal Magro Roberta , Ceriani Michela , Re Francesca
Published: August 2021

Glioblastoma (GBM) is a particularly challenging brain tumor characterized by a heterogeneous, complex, and multicellular microenvironment, which represents a strategic network for treatment escape. Furthermore, the presence of GBM stem cells (GSCs) seems to contribute to GBM recurrence after surgery, and chemo- and/or radiotherapy. In this context, intercellular communication modalities play key roles in driving GBM therapy resistance. The presence of tunneling nanotubes (TNTs), long membranous open-ended channels connecting distant cells, has been observed in several types of cancer, where they emerge to steer a more malignant phenotype. Here, we discuss the current knowledge about the formation of TNTs between different cellular types in the GBM microenvironment and their potential role in tumor progression and recurrence. Particularly, we highlight two prospective strategies targeting TNTs as possible therapeutics: (i) the inhibition of TNT formation and (ii) a boost in drug delivery between cells through these channels. The latter may require future studies to design drug delivery systems that are exchangeable through TNTs, thus allowing for access to distant tumor niches that are involved in tumor immune escape, maintenance of GSC plasticity, and increases in metastatic potential.
IL-27 Mediates PD-L1 Expression and Release by Human Mesothelioma Cells
Authors: Carbotti Grazia , Dozin Beatrice , Martini Stefania , Giordano Chiara , Scordamaglia Francesca , Croce Michela , Filaci Gilberto , Ferrini Silvano , Fabbi Marina
Published: August 2021

Malignant mesothelioma (MM) is a rare tumor with an unfavorable prognosis. MM genesis involves asbestos-mediated local inflammation, supported by several cytokines, including IL-6. Recent data showed that targeting PD-1/PD-L1 is an effective therapy in MM. Here, we investigated the effects of IL-6 trans-signaling and the IL-6-related cytokine IL-27 on human MM cells in vitro by Western blot analysis of STAT1/3 phosphorylation. The effects on PD-L1 expression were tested by qRT-PCR and flow-cytometry and the release of soluble (s)PD-L1 by ELISA. We also measured the concentrations of sPD-L1 and, by multiplexed immunoassay, IL-6 and IL-27 in pleural fluids obtained from 77 patients in relation to survival. IL-27 predominantly mediates STAT1 phosphorylation and increases PD-L1 gene and surface protein expression and sPD-L1 release by human MM cells in vitro. IL-6 has limited activity, whereas a sIL-6R/IL-6 chimeric protein mediates trans-signaling predominantly via STAT3 phosphorylation but has no effect on PD-L1 expression and release. IL-6, IL-27, and sPD-L1 are present in pleural fluids and show a negative correlation with overall survival, but only IL-27 shows a moderate albeit significant correlation with sPD-L1 levels. Altogether these data suggest a potential role of IL-27 in PD-L1-driven immune resistance in MM.
Functionalized and Platinum-Decorated Multi-Layer Oxidized Graphene as a Proton, and Electron Conducting Separator in Solid Acid Fuel Cells
Authors: Hatahet Mhamad Hamza , Wagner Maximilian , Prager Andrea , Helmstedt Ulrike , Abel Bernd
Published: August 2021

In the present article, electrodes containing a composite of platinum on top of a plasma-oxidized multi-layer graphene film are investigated as model electrodes that combine an exceptional high platinum utilization with high electrode stability. Graphene is thereby acting as a separator between the phosphate-based electrolyte and the platinum catalyst. Electrochemical impedance measurements in humidified hydrogen at 240 °C show area-normalized electrode resistance of 0.06 Ω·cm−2 for a platinum loading of ∼60 µgPt·cm−2, resulting in an outstanding mass normalized activity of almost 280 S·mgPt−1, exceeding even state-of-the-art electrodes. The presented platinum decorated graphene electrodes enable stable operation over 60 h with a non-optimized degradation rate of 0.15% h−1, whereas electrodes with a similar design but without the graphene as separator are prone to a very fast degradation. The presented results propose an efficient way to stabilize solid acid fuel cell electrodes and provide valuable insights about the degradation processes which are essential for further electrode optimization.
The Role of Galanin during Bacterial Infection in Larval Zebrafish
Authors: Nowik Natalia , Prajsnar Tomasz K. , Przyborowska Anna , Rakus Krzysztof , Sienkiewicz Waldemar , Spaink Herman P. , Podlasz Piotr
Published: August 2021

Galanin is a peptide that is conserved among different species and plays various roles in an organism, although its entire role is not completely understood. For many years, galanin has been linked mainly with the neurotransmission in the nervous system; however, recent reports underline its role in immunity. Zebrafish (Danio rerio) is an intensively developing animal model to study infectious diseases. In this study, we used larval zebrafish to determine the role of galanin in bacterial infection. We showed that knockout of galanin in zebrafish leads to a higher bacterial burden and mortality during Mycobacterium marinum and Staphylococcus aureus infection, whereas administration of a galanin analogue, NAX 5055, improves the ability of fish to control the infection caused by both pathogens. Moreover, the transcriptomics data revealed that a lower number of genes were regulated in response to mycobacterial infection in gal−/− mutants compared with their gal+/+ wild-type counterparts. We also found that galanin deficiency led to significant changes in immune-related pathways, mostly connected with cytokine and chemokine functions. The results show that galanin acts not only as a neurotransmitter but is also involved in immune response to bacterial infections, demonstrating the complexity of the neuroendocrine system and its possible connection with immunity.