bluuebunny/crossref_metadata_2025 huggingface dataset:
Turkish pre-service early childhood education teachers’ perceptions and practices of nature-based teaching on professional experience
Authors: Karaarslan Semiz Güliz , Temiz Zeynep
Published: June 2021

This paper considers the perceptions and teaching practices of Turkish pre-service early childhood education teachers with regard to nature-based teaching. Nature-based activities rarely take place in Turkish early childhood education settings; accordingly, there is a clear need to develop pre-service teachers’ professional experience related to nature-based teaching to empower them to support children’s learning in natural environments. In this study, eight pre-service teachers applied nature-based teaching in a professional experience placement over a period of 10 weeks. Clarke and Peterson’s (1986) model of teachers’ thoughts and actions guided this study in terms of understanding pre-service teachers’ related perceptions and practices. Qualitative data were collected through activity plans and semi-structured interviews, the findings from which revealed that the implementation of various activities using natural materials and the natural environment increased pre-service teachers’ self-confidence related to nature-based teaching. The primary barriers to the implementation of nature-based teaching activities were reported as being the attitudes of families and school management, weather conditions and pre-service teachers’ lack of knowledge of nature-based pedagogies. These barriers could be addressed by supporting pre-service early childhood teachers’ professional development and by affording them more opportunities to perform nature-related activities in early childhood settings.
Activating Animus: The Uniquely Social Roots of Trump Support
Authors: MASON LILLIANA , WRONSKI JULIE , KANE JOHN V.
Published: June 2021

Partisanship in American politics is inextricably linked with social identities, and sentiments toward party-aligned groups affect political orientations. However, out-group animosity may operate differently depending on the party or elite. We investigate the extent to which citizens’ animus toward (Democratically aligned) minority groups drove political support for Donald Trump, whose incendiary rhetoric regarding such groups is unique in modern presidential politics. Leveraging panel data beginning before Trump’s candidacy, we find that animus toward Democratic-linked groups in 2011 predicts future support for Trump regardless of party identity. This animus does not predict future support for other Republican or Democratic politicians or either party. Nor do we find that animus toward Republican groups predicts support for Democratic elites. Trump’s support is thus uniquely tied to animus toward minority groups. Our findings provide insights into the social divisions underlying American politics and the role of elite rhetoric in translating animus into political support.
“Greetings from the Apocalypse”: Race, Migration, and Fear after German Reunification
Authors: Molnar Christopher A.
Published: June 2021

In the early 1990s, reunified Germany faced surging numbers of asylum seekers and a wave of far-right violence against foreigners. In letters that German citizens wrote to President Richard von Weizsäcker, it is clear that some Germans feared that the arrival of large numbers of non-Germans would bring about the collapse of the state or the destruction of the German people. This article engages with the history of fear in postwar Germany by examining Germans’ post-reunification fears of migration and foreigners. I argue, first, that a biological understanding of race was still surprisingly widespread as late as the early 1990s. Second, I call for a substantially more nuanced understanding of German reunification, one that emphasizes uncertainty and the terrifying openness of the future. Finally, I highlight the intersection of race thinking and memory by showing that racial fears were frequently shaped by memories of Germany's dark past.
Detecting wildlife poaching: a rigorous method for comparing patrol strategies using an experimental design
Authors: van Doormaal Nick , Lemieux A. M. , Ruiter Stijn , Allin Paul M. R. R. , Spencer Craig R.
Published: June 2021

Many studies of wildlife poaching acknowledge the challenges of detecting poaching activities, but few address the issue. Data on poaching may be an inaccurate reflection of the true spatial distribution of events because of low detection rates. The deployment of conservation and law enforcement resources based on biased data could be ineffective or lead to unintended outcomes. Here, we present a rigorous method for estimating the probabilities of detecting poaching and for evaluating different patrol strategies. We illustrate the method with a case study in which imitation snares were set in a private nature reserve in South Africa. By using an experimental design with a known spatial distribution of imitation snares, we estimated the detection probability of the current patrol strategy used in the reserve and compared it to three alternative patrol strategies: spatially focused patrols, patrols with independent observers, and systematic search patterns. Although detection probabilities were generally low, the highest proportion of imitation snares was detected with systematic search strategies. Our study provides baseline data on the probability of detecting snares used for poaching, and presents a method that can be modified for use in other regions and for other types of wildlife poaching.
Classifications at Work: Social Categories and Dutch Bureaucracy in Colonial Sri Lanka
Authors: Lyna Dries , Bulten Luc
Published: June 2021

Feeding into current debates on ethnic identities in colonial South Asia, this article questions to what extent Dutch institutions articulated and impacted social categories of people living in coastal Sri Lanka during the eighteenth century. A thorough analysis of three spheres of Dutch bureaucracy (reporting, registering, and litigating) makes it clear that there was no uniform ideology that steered categorisation practices top-down throughout the studied colonial institutions. Rather, the rationale of the organisation as such affected the way people were classified, depending to a large extent on what level of bureaucracy individuals were dealing with, and what the possible negotiation strategies were for the people recorded. Future research should perhaps not ask “when” certain ethnicities were “made up,” but strive to understand the process in which they were created, the institutional contexts in which they were recorded, and how changing bureaucratic practices not only articulated, but also transformed, social categories in the long run.
Does the Presence of On-Site Medical Services at Outdoor Music Festivals Affect Attendees’ Planned Alcohol and Recreational Drug Use?
Authors: Hutton Alison , Munn Matthew Brendan , White Sydney , Kara Peter , Ranse Jamie
Published: June 2021

Background:

Dedicated on-site medical services have long been recommended to improve health outcomes at mass-gathering events (MGEs). In many countries, they are being reviewed as a mandatory requirement. While it is known that perceptions of risk shape substance use plans amongst outdoor music festival (OMF) attendees, it is unclear if attendees perceive the presence of on-site medical services as a part of the safety net. The aim of this paper is to better understand whether attendees’ perceptions of on-site medical services influence high-risk behaviors like alcohol and recreational drug use at OMFs.

Method:

A questionnaire was distributed to a random sample of attendees entering and attending two separate 20,000-person OMFs; one in Canada (Festival A) and one in New Zealand (Festival B). Responses focused on demographics, planned alcohol and recreational drug use, perceptions of medical services, and whether the absence of medical services would impact attendees’ planned substance use.

Results:

A total of 851 (587 and 264 attendees for Festival A and Festival B, respectively) attendees consented and participated. Gender distribution was equal and average ages were 23 to 25. At Festival A, 48% and 89% planned to use alcohol and recreational drugs, respectively, whereas at Festival B, it was 92% and 44%. A great majority were aware and supportive of the presence of medical services at both festivals, and a moderate number considered them a factor in attendance and something they would not attend without. There was significant (>10%) agreement (range 11%-46%; or 2,200-9,200 attendees for a 20,000-person festival) at both festivals that the absence of medical services would affect attendees’ planned use of alcohol and recreational drugs.

Conclusions:

This study found that attendees surveyed at two geographically and musically distinct OMFs had high but differing rates of planned alcohol and recreational drug use, and that the presence of on-site medical services may impact attendees’ perceptions of substance use risk. Future research will aim to address the limitations of this study to clarify these findings and their implications.
THE TRANSFORMATION OF ECONOMIC ANALYSIS AT THE BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM DURING THE 1960S
Authors: Acosta Juan , Cherrier Beatrice
Published: June 2021

In this paper, we build on data on officials of the Federal Reserve System, oral history repositories, and hitherto underresearched archival sources to unpack the tortuous path toward crafting an institutional and intellectual space for postwar economic analysis within the Board of Governors of the Federal Reserve System. We show that growing attention to new macroeconomic research was a reaction to both mounting external criticisms against the Fed’s decision-making process and the spread of new macroeconomic theories and econometric techniques. We argue that the rise of the number of PhD economists working at the Fed is a symptom rather than a cause of this transformation. Key to our story are a handful of economists from the Board of Governors’ Division of Research and Statistics (DRS) who did not hold a PhD but envisioned their role as going beyond mere data accumulation and got involved in large-scale macroeconometric model building. We conclude that the divide between PhD and non-PhD economists may not be fully relevant to understand both the shift in the type of economics practiced at the Fed and the uses of this knowledge in the decision-making process. Equally important was the rift between different styles of economic analysis.
Impact of Fracture Geometry and Topology on the Connectivity and Flow Properties of Stochastic Fracture Networks
Authors: Zhu Weiwei , Khirevich Siarhei , Patzek Tad W.
Published: June 2021

In a low permeability formation, connectivity of natural and induced fractures determines overall hydraulic diffusivity in fluid flow through this formation and defines effective rock permeability. Efficient evaluation of fracture connectivity is a nontrivial task. Here, we utilize a topological concept of global efficiency to evaluate this connectivity. We address the impact of key geometrical properties of stochastic fracture networks (fracture lengths, orientations, apertures, and positions of fracture centers) on the macro‐scale flow properties of a shale‐like formation. Six thousand different realizations have been generated to characterize these properties for each fracture network. We find that a reduced graph of a fracture network, which consists of the shortest paths from the inlet nodes (fractures) to all outlet nodes, contributes most to fluid flow. Three‐dimensional (3D) fracture networks usually have higher global efficiency than two‐dimensional (2D) ones, because they have better connectivity. All geometrical properties of fractures influence quality of their connectivity. Aperture distribution impacts strongly global efficiency of a fracture network and its influence is more significant when the system is dominated by large fractures. Fracture clustering lowers global efficiency in both 2D and 3D fracture networks. Global efficiency of 2D and 3D fracture networks also decreases with the increasing exponent of the power‐law distribution of fracture lengths, which means that the connectivity of the system decreases with an increasing number of small fractures. Realistic fracture networks, composed of several sets of fractures with constrained preferred orientations, share all the characteristics of the stochastic fracture networks we have investigated.
Characteristics of Resonant Electrons Interacting With Whistler Waves in the Nearest Dipolarizing Magnetotail
Authors: Malykhin A. Y. , Grigorenko E. E. , Shklyar D. R. , Panov E. V. , Le Contel O. , Avanov L. , Giles B.
Published: June 2021

The Magnetospheric Multiscale spacecraft observations in the burst mode allow the determination of the characteristics of resonant electrons interacting with quasi‐parallel whistler waves during prolonged dipolarizations in the near Earth magnetotail at −22 RE < X ≤ −8 RE. We have detected 163 whistler bursts observed during 48 dipolarization events. The bursts were registered within ∼13 min following the dipolarization onset when the burst mode observations were available. In the majority of events, electrons with energies Wres ≥ 10 keV and pitch angles αres ∼ 100°–130° and αres ∼ 50°–80°made the maximum positive contribution to the growth rate of whistler waves propagating quasi‐parallel and antiparallel to the ambient magnetic field, respectively. Our analysis shows that electrons with Wres ∼ 10–20 keV could potentially be scattered into the loss cone by the low frequency whistler waves with fw ∼ (0.05–0.2)fce (fw is the wave frequency and fce is the electron gyrofrequency). The electrons that are scattered into the loss cone can contribute to electron precipitation within ∼13 min following the dipolarization onset. We suggest that whistler waves that are excited due to cyclotron instability driven by the temperature anisotropy of suprathermal electrons (≥2 keV) may, in turn, affect electron distribution in this energy range. Specifically, lower energy resonant electrons transfer a part of their kinetic energy to waves, while more energetic electrons absorb wave energy increasing their kinetic energy. This may lead to the transformation of higher‐energy part of electron distribution from Maxwellian to the power law shape.
Observational Evidence of Ventilation Hotspots in the Southern Ocean
Authors: Dove Lilian A. , Thompson Andrew F. , Balwada Dhruv , Gray Alison R.
Published: June 2021

Standing meanders are a key component of the Antarctic Circumpolar Current (ACC) circulation system, and numerical studies have shown that these features may locally enhance subduction, upwelling, as well as lateral and vertical tracer transport. Yet, observational data from these regions remain sparse. Here, we present results based on measurements made by a group of autonomous platforms sampling an ACC standing meander formed due to the interaction of the Polar Front with the Southwest Indian Ridge. Two Seagliders were deployed alongside a Biogeochemical‐Argo float that was advected through the standing meander. In the high eddy kinetic energy region of the standing meander, the glider observations reveal enhanced submesoscale frontal gradients as well as heightened tracer variability at depth, as compared to the more quiescent region further downstream. Vertical gradients in spice and apparent oxygen utilization are reduced in the standing meander despite similarities in the large‐scale vertical stratification, suggesting greater ventilation of the surface ocean. These observations are consistent with numerical studies that highlight standing meanders as hotspots for ventilation and subduction due to enhanced mesoscale stirring and submesoscale vertical velocities. Our results emphasize the need to account for spatial heterogeneity in processes influencing air‐sea exchange, carbon export, and biogeochemical cycling in the Southern Ocean.
Subtropical Eastern North Pacific SST Bias in Earth System Models
Authors: Balaguru Karthik , Van Roekel Luke P. , Leung L. Ruby , Veneziani Milena
Published: June 2021

This study systematically evaluates the warm sea surface temperature (SST) bias in the Subtropical Eastern North Pacific, a problem plaguing most Coupled Model Intercomparison Project Phase 6 models, using the Energy Exascale Earth System Model version 1 (E3SM). In the model at its standard resolution (1° atmosphere, 30–60 km ocean), the SST bias, exceeding several degrees, is mainly concentrated along the coast between 25°N and 40°N. In the high‐resolution (0.25° atmosphere, 18–6 km ocean) version of the model, the nearshore SST bias improves considerably with a better representation of coastal upwelling. However, the offshore SST bias, approximately centered at 125°W and 25°N, is relatively stronger in the high‐resolution version. To better understand the offshore warm bias in the model, a mixed‐layer heat budget analysis is performed. While errors in surface radiative fluxes occur at both resolutions, positive biases in horizontal heat advection also play a role in the SST bias at high‐resolution. Analysis of HighResMIP models indicates that the shift in the location of the prominent SST bias from nearshore to offshore with an increase in model spatial resolution, is not native to E3SM alone.
Insights Into Water Mass Origins in the Central Arctic Ocean From In‐Situ Dissolved Organic Matter Fluorescence
Authors: Stedmon Colin A. , Amon Rainer M. W. , Bauch Dorothea , Bracher Astrid , Gonçalves‐Araujo Rafael , Hoppmann Mario , Krishfield Richard , Laney Samuel , Rabe Benjamin , Reader Heather , Granskog Mats A.
Published: June 2021

The Arctic Ocean receives a large supply of dissolved organic matter (DOM) from its catchment and shelf sediments, which can be traced across much of the basin's upper waters. This signature can potentially be used as a tracer. On the shelf, the combination of river discharge and sea‐ice formation, modifies water densities and mixing considerably. These waters are a source of the halocline layer that covers much of the Arctic Ocean, but also contain elevated levels of DOM. Here we demonstrate how this can be used as a supplementary tracer and contribute to evaluating ocean circulation in the Arctic. A fraction of the organic compounds that DOM consists of fluoresce and can be measured using in‐situ fluorometers. When deployed on autonomous platforms these provide high temporal and spatial resolution measurements over long periods. The results of an analysis of data derived from several Ice Tethered Profilers (ITPs) offer a unique spatial coverage of the distribution of DOM in the surface 800 m below Arctic sea‐ice. Water mass analysis using temperature, salinity and DOM fluorescence, can clearly distinguish between the contribution of Siberian terrestrial DOM and marine DOM from the Chukchi shelf to the waters of the halocline. The findings offer a new approach to trace the distribution of Pacific waters and its export from the Arctic Ocean. Our results indicate the potential to extend the approach to separate freshwater contributions from, sea‐ice melt, riverine discharge and the Pacific Ocean.
A narrative overview of utilizing biomaterials to recapitulate the salient regenerative features of dental-derived mesenchymal stem cells
Authors: Sevari Sevda Pouraghaei , Ansari Sahar , Moshaverinia Alireza
Published: June 2021

Tissue engineering approaches have emerged recently to circumvent many limitations associated with current clinical practices. This elegant approach utilizes a natural/synthetic biomaterial with optimized physiomechanical properties to serve as a vehicle for delivery of exogenous stem cells and bioactive factors or induce local recruitment of endogenous cells for in situ tissue regeneration. Inspired by the natural microenvironment, biomaterials could act as a biomimetic three-dimensional (3D) structure to help the cells establish their natural interactions. Such a strategy should not only employ a biocompatible biomaterial to induce new tissue formation but also benefit from an easily accessible and abundant source of stem cells with potent tissue regenerative potential. The human teeth and oral cavity harbor various populations of mesenchymal stem cells (MSCs) with self-renewing and multilineage differentiation capabilities. In the current review article, we seek to highlight recent progress and future opportunities in dental MSC-mediated therapeutic strategies for tissue regeneration using two possible approaches, cell transplantation and cell homing. Altogether, this paper develops a general picture of current innovative strategies to employ dental-derived MSCs combined with biomaterials and bioactive factors for regenerating the lost or defective tissues and offers information regarding the available scientific data and possible applications.
Direct observation of chaotic resonances in optical microcavities
Authors: Wang Shuai , Liu Shuai , Liu Yilin , Xiao Shumin , Wang Zi , Fan Yubin , Han Jiecai , Ge Li , Song Qinghai
Published: June 2021

Optical microcavities play a significant role in the study of classical and quantum chaos. To date, most experimental explorations of their internal wave dynamics have focused on the properties of their inputs and outputs, without directly interrogating the dynamics and the associated mode patterns inside. As a result, this key information is rarely retrieved with certainty, which significantly restricts the verification and understanding of the actual chaotic motion. Here we demonstrate a simple and robust approach to directly and rapidly map the internal mode patterns in chaotic microcavities. By introducing a local index perturbation through a pump laser, we report a spectral response of optical microcavities that is proportional to the internal field distribution. With this technique, chaotic modes with staggered mode spacings can be distinguished. Consequently, a complete chaos assisted tunneling (CAT) and its time-reversed process are experimentally verified in the optical domain with unprecedented certainty.
Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide
Authors: Ng Sheung Chun , Güttler Thomas , Görlich Dirk
Published: June 2021

The permeability barrier of nuclear pore complexes (NPCs) controls nucleocytoplasmic transport. It retains inert macromolecules while allowing facilitated passage of importins and exportins, which in turn shuttle cargo into or out of cell nuclei. The barrier can be described as a condensed phase assembled from cohesive FG repeat domains. NPCs contain several distinct FG domains, each comprising variable repeats. Nevertheless, we now found that sequence heterogeneity is no fundamental requirement for barrier function. Instead, we succeeded in engineering a perfectly repeated 12mer GLFG peptide that self-assembles into a barrier of exquisite transport selectivity and fast transport kinetics. This barrier recapitulates RanGTPase-controlled importin- and exportin-mediated cargo transport and thus represents an ultimately simplified experimental model system. An alternative proline-free sequence forms an amyloid FG phase. Finally, we discovered that FG phases stain bright with ‘DNA-specific’ DAPI/ Hoechst probes, and that such dyes allow for a photo-induced block of nuclear transport.
Higher cost of finance exacerbates a climate investment trap in developing economies
Authors: Ameli Nadia , Dessens Olivier , Winning Matthew , Cronin Jennifer , Chenet Hugues , Drummond Paul , Calzadilla Alvaro , Anandarajah Gabrial , Grubb Michael
Published: June 2021

Finance is vital for the green energy transition, but access to low cost finance is uneven as the cost of capital differs substantially between regions. This study shows how modelled decarbonisation pathways for developing economies are disproportionately impacted by different weighted average cost of capital (WACC) assumptions. For example, representing regionally-specific WACC values indicates 35% lower green electricity production in Africa for a cost-optimal 2 °C pathway than when regional considerations are ignored. Moreover, policy interventions lowering WACC values for low-carbon and high-carbon technologies by 2050 would allow Africa to reach net-zero emissions approximately 10 years earlier than when the cost of capital reduction is not considered. A climate investment trap arises for developing economies when climate-related investments remain chronically insufficient. Current finance frameworks present barriers to these finance flows and radical changes are needed so that capital is more equitably distributed.
The rate and molecular spectrum of mutation are selectively maintained in yeast
Authors: Liu Haoxuan , Zhang Jianzhi
Published: June 2021

What determines the rate (μ) and molecular spectrum of mutation is a fundamental question. The prevailing hypothesis asserts that natural selection against deleterious mutations has pushed μ to the minimum achievable in the presence of genetic drift, or the drift barrier. Here we show that, contrasting this hypothesis, μ substantially exceeds the drift barrier in diverse organisms. Random mutation accumulation (MA) in yeast frequently reduces μ, and deleting the newly discovered mutator gene PSP2 nearly halves μ. These results, along with a comparison between the MA and natural yeast strains, demonstrate that μ is maintained above the drift barrier by stabilizing selection. Similar comparisons show that the mutation spectrum such as the universal AT mutational bias is not intrinsic but has been selectively preserved. These findings blur the separation of mutation from selection as distinct evolutionary forces but open the door to alleviating mutagenesis in various organisms by genome editing.
SARS-CoV-2-specific T cell memory is sustained in COVID-19 convalescent patients for 10 months with successful development of stem cell-like memory T cells
Authors: Jung Jae Hyung , Rha Min-Seok , Sa Moa , Choi Hee Kyoung , Jeon Ji Hoon , Seok Hyeri , Park Dae Won , Park Su-Hyung , Jeong Hye Won , Choi Won Suk , Shin Eui-Cheol
Published: June 2021

Memory T cells contribute to rapid viral clearance during re-infection, but the longevity and differentiation of SARS-CoV-2-specific memory T cells remain unclear. Here we conduct ex vivo assays to evaluate SARS-CoV-2-specific CD4+ and CD8+ T cell responses in COVID-19 convalescent patients up to 317 days post-symptom onset (DPSO), and find that memory T cell responses are maintained during the study period regardless of the severity of COVID-19. In particular, we observe sustained polyfunctionality and proliferation capacity of SARS-CoV-2-specific T cells. Among SARS-CoV-2-specific CD4+ and CD8+ T cells detected by activation-induced markers, the proportion of stem cell-like memory T (TSCM) cells is increased, peaking at approximately 120 DPSO. Development of TSCM cells is confirmed by SARS-CoV-2-specific MHC-I multimer staining. Considering the self-renewal capacity and multipotency of TSCM cells, our data suggest that SARS-CoV-2-specific T cells are long-lasting after recovery from COVID-19, thus support the feasibility of effective vaccination programs as a measure for COVID-19 control.
Tropical deforestation induces thresholds of reproductive viability and habitat suitability in Earth’s largest eagles
Authors: Miranda Everton B. P. , Peres Carlos A. , Carvalho-Rocha Vítor , Miguel Bruna V. , Lormand Nickolas , Huizinga Niki , Munn Charles A. , Semedo Thiago B. F. , Ferreira Tiago V. , Pinho João B. , Piacentini Vítor Q. , Marini Miguel Â. , Downs Colleen T.
Published: June 2021

Apex predators are threatened globally, and their local extinctions are often driven by failures in sustaining prey acquisition under contexts of severe prey scarcity. The harpy eagleHarpia harpyjais Earth’s largest eagle and the apex aerial predator of Amazonian forests, but no previous study has examined the impact of forest loss on their feeding ecology. We monitored 16 active harpy eagle nests embedded within landscapes that had experienced 0 to 85% of forest loss, and identified 306 captured prey items. Harpy eagles could not switch to open-habitat prey in deforested habitats, and retained a diet based on canopy vertebrates even in deforested landscapes. Feeding rates decreased with forest loss, with three fledged individuals dying of starvation in landscapes that succumbed to 50–70% deforestation. Because landscapes deforested by > 70% supported no nests, and eaglets could not be provisioned to independence within landscapes > 50% forest loss, we established a 50% forest cover threshold for the reproductive viability of harpy eagle pairs. Our scaling-up estimate indicates that 35% of the entire 428,800-km2Amazonian ‘Arc of Deforestation’ study region cannot support breeding harpy eagle populations. Our results suggest that restoring harpy eagle population viability within highly fragmented forest landscapes critically depends on decisive forest conservation action.
Identification of potential microRNAs and KEGG pathways in denervation muscle atrophy based on meta-analysis
Authors: Gu Xinyi , Jin Bo , Qi Zhidan , Yin Xiaofeng
Published: June 2021

The molecular mechanism of muscle atrophy has been studied a lot, but there is no comprehensive analysis focusing on the denervated muscle atrophy. The gene network that controls the development of denervated muscle atrophy needs further elucidation. We examined differentially expressed genes (DEGs) from five denervated muscle atrophy microarray datasets and predicted microRNAs that target these DEGs. We also included the differentially expressed microRNAs datasets of denervated muscle atrophy in previous studies as background information to identify potential key microRNAs. Finally, we compared denervated muscle atrophy with disuse muscle atrophy caused by other reasons, and obtained the Den-genes which only differentially expressed in denervated muscle atrophy. In this meta-analysis, we obtained 429 up-regulated genes, 525 down-regulated genes and a batch of key microRNAs in denervated muscle atrophy. We found eight important microRNA-mRNA interactions (miR-1/Jun, miR-1/Vegfa, miR-497/Vegfa, miR-23a/Vegfa, miR-206/Vegfa, miR-497/Suclg1, miR-27a/Suclg1, miR-27a/Mapk14). The top five KEGG pathways enriched by Den-genes are Insulin signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway and B cell receptor signaling pathway. Our research has delineated the RNA regulatory network of denervated muscle atrophy, and uncovered the specific genes and terms in denervated muscle atrophy.
Identifying ENSO-related interannual and decadal variability on terrestrial water storage
Authors: Cheon Se-Hyeon , Hamlington Benjamin D. , Reager John T. , Chandanpurkar Hrishikesh A.
Published: June 2021

We apply two statistical techniques to satellite measurements to identify a relationship between terrestrial water storage (TWS) and El Niño-Southern Oscillation (ENSO). First, we modified and used the least-squares regression of a previous study using longer records. Second, we applied a cyclostationary empirical orthogonal function analysis (CSEOF). Although the CSEOF technique is distinct from the least-squares regression in that it does not consider proxies, each method produces two modes (decadal and interannual), showing consistency with each technique in spatial pattern and its evolution amplitudes. We also compared the results obtained by the two methods for thirty watersheds, of which five watersheds were compared with previous studies. The combination of the two modes explains the total variance in most watersheds showing the role that interannual and decadal ENSO-related signals in understanding terrestrial water storage variability. The results show that the decadal mode, along with the interannual mode, also plays an important role in describing the local TWS.
Combining microfluidics with machine learning algorithms for RBC classification in rare hereditary hemolytic anemia
Authors: Rizzuto Valeria , Mencattini Arianna , Álvarez-González Begoña , Di Giuseppe Davide , Martinelli Eugenio , Beneitez-Pastor David , Mañú-Pereira Maria del Mar , Lopez-Martinez Maria José , Samitier Josep
Published: June 2021

Combining microfluidics technology with machine learning represents an innovative approach to conduct massive quantitative cell behavior study and implement smart decision-making systems in support of clinical diagnostics. The spleen plays a key-role in rare hereditary hemolytic anemia (RHHA), being the organ responsible for the premature removal of defective red blood cells (RBCs). The goal is to adapt the physiological spleen filtering strategy for in vitro study and monitoring of blood diseases through RBCs shape analysis. Then, a microfluidic device mimicking the slits of the spleen red pulp area and video data analysis are combined for the characterization of RBCs in RHHA. This microfluidic unit is designed to evaluate RBC deformability by maintaining them fixed in planar orientation, allowing the visual inspection of RBC’s capacity to restore their original shape after crossing microconstrictions. Then, two cooperative learning approaches are used for the analysis: the majority voting scheme, in which the most voted label for all the cell images is the class assigned to the entire video; and the maximum sum of scores to decide the maximally scored class to assign. The proposed platform shows the capability to discriminate healthy controls and patients with an average efficiency of 91%, but also to distinguish between RHHA subtypes, with an efficiency of 82%.
Contrasting alien effects on native diversity along biotic and abiotic gradients in an arid protected area
Authors: El-Barougy Reham F. , Elgamal Ibrahim A. , Khedr Abdel-Hamid A. , Bersier Louis-Félix
Published: June 2021

Alien impact on native diversity could be a function of both the relatedness of alien species to native community and resources availability. Here, we investigated whether alien plants expand or decrease the functional and phylogenetic space of native plant communities, and how this is affected by alien relatedness to natives and by resources availability. We used a trait-environment dataset of 33 alien and 130 native plants in 83 pairs of invaded and non-invaded plots, covering a gradient of soil resources (organic matter-nitrogen) in Saint-Katherine-Protectorate, Egypt. First, we compared the changes in native composition and calculated alien relatedness to natives within each pair of plots. Second, we tested the effects of resources availability and relatedness on the magnitude of alien impact (defined as a change in native diversity). We found that native composition was phylogenetically less but functionally more diverse in invaded plots compared to non-invaded ones. Moreover, in resources-rich plots, dissimilar aliens to natives significantly increased native diversity, while in resource-limited ones, similar aliens to natives declined native diversity. These results suggest that the assessment of alien impacts in arid-regions is significantly linked to resources-availability and relatedness to natives. Hence, future studies should test the generality of our findings in different environments.
Controlled creation and annihilation of isolated robust emergent magnetic monopole like charged vertices in square artificial spin ice
Authors: Keswani Neeti , Lopes Ricardo J. C. , Nakajima Yoshikata , Singh Ranveer , Chauhan Neha , Som Tapobrata , Kumar D. Sakthi , Pereira Afranio R. , Das Pintu
Published: June 2021

Magnetic analogue of an isolated free electric charge, i.e., a magnet with a single north or south pole, is a long sought-after particle which remains elusive so far. In magnetically frustrated pyrochlore solids, a classical analogue of monopole was observed as a result of excitation of spin ice vertices. Direct visualization of such excitations were proposed and later confirmed in analogous artificial spin ice (ASI) systems of square as well as Kagome geometries. However, such magnetically charged vertices are randomly created as they are thermally driven and are always associated with corresponding equal and opposite emergent charges, often termed as monopole–antimonopole pairs, connected by observable strings. Here, we demonstrate a controlled stabilisation of a robust isolated emergent monopole-like magnetically charged vertices in individual square ASI systems by application of an external magnetic field. The excitation conserves the magnetic charge without the involvement of a corresponding excitation of opposite charge. Well supported by Monte Carlo simulations our experimental results enable, in absence of a true elemental magnetic monopole, creation of electron vortices and studying electrodynamics in presence of a monopole-like field in a solid state environment.
Examining early structural and functional brain alterations in postpartum depression through multimodal neuroimaging
Authors: Schnakenberg Patricia , Hahn Lisa , Stickel Susanne , Stickeler Elmar , Habel Ute , Eickhoff Simon B. , Chechko Natalia , Dukart Juergen
Published: June 2021

Postpartum depression (PPD) affects approximately 1 in 10 women after childbirth. A thorough understanding of a preexisting vulnerability to PPD will likely aid the early detection and treatment of PPD. Using a within-sample association, the study examined whether the brain’s structural and functional alterations predict the onset of depression. 157 euthymic postpartum women were subjected to a multimodal MRI scan within the first 6 days of childbirth and were followed up for 12 weeks. Based on a clinical interview 12 weeks postpartum, participants were classified as mentally healthy or having either PPD or adjustment disorder (AD). Voxel-based morphometry and resting-state functional connectivity comparisons were performed between the three groups. 13.4% of women in our study developed PPD (n = 21) and 12.1% (n = 19) adjustment disorder (AD). The risk factors for PPD were a psychiatric history and the experience and severity of baby blues and the history of premenstrual syndrome. Despite the different risk profiles, no differences between the PPD, AD and control group were apparent based on structural and functional neuroimaging data immediately after childbirth. At 12 weeks postpartum, a significant association was observed between Integrated Local Correlation (LCor) and the Edinburgh Postnatal Depression Score (EPDS). Our findings do not support the notion that the brain’s structural and resting-state functional alterations, if present, can be used as an early biomarker of PPD or AD. However, effects may become apparent if continuous measures of symptom severity are chosen.
Quantifying finer-scale behaviours using self-organising maps (SOMs) to link accelerometery signatures with behavioural patterns in free-roaming terrestrial animals
Authors: Galea Nicole , Murphy Fern , Gaschk Joshua L. , Schoeman David S. , Clemente Christofer J.
Published: June 2021

Collecting quantitative information on animal behaviours is difficult, especially from cryptic species or species that alter natural behaviours under observation. Using harness-mounted tri-axial accelerometers free-roaming domestic cats (Felis Catus) we developed a methodology that can precisely classify finer-scale behaviours. We further tested the effect of a prey–protector device designed to reduce prey capture. We aligned accelerometer traces collected at 50 Hz with video files (60 fps) and labelled 12 individual behaviours, then trained a supervised machine-learning algorithm using Kohonen super self-organising maps (SOM). The SOM was able to predict individual behaviours with a ~ 99.6% overall accuracy, which was slightly better than for random forest estimates using the same dataset (98.9%). There was a significant effect of sample size, with precision and sensitivity decreasing rapidly below 2000 1-s observations. We were also able to detect a behaviour specific reduction in the predictability when cats were fitted with the prey–protector device indicating it altered biomechanical gait. Our results can be applied in movement ecology, zoology and conservation, where habitat specific movement performance between predators or prey may be critical to managing species of conservation significance, or in veterinary and agricultural fields, where early detection of movement pathologies can improve animal welfare.
An empirical investigation of trust in AI in a Chinese petrochemical enterprise based on institutional theory
Authors: Li Jia , Zhou Yiwen , Yao Junping , Liu Xuan
Published: June 2021

Despite its considerable potential in the manufacturing industry, the application of artificial intelligence (AI) in the industry still faces the challenge of insufficient trust. Since AI is a black box with operations that ordinary users have difficulty understanding, users in organizations rely on institutional cues to make decisions about their trust in AI. Therefore, this study investigates trust in AI in the manufacturing industry from an institutional perspective. We identify three institutional dimensions from institutional theory and conceptualize them as management commitment (regulative dimension at the organizational level), authoritarian leadership (normative dimension at the group level), and trust in the AI promoter (cognitive dimension at the individual level). We hypothesize that all three institutional dimensions have positive effects on trust in AI. In addition, we propose hypotheses regarding the moderating effects of AI self-efficacy on these three institutional dimensions. A survey was conducted in a large petrochemical enterprise in eastern China just after the company had launched an AI-based diagnostics system for fault detection and isolation in process equipment service. The results indicate that management commitment, authoritarian leadership, and trust in the AI promoter are all positively related to trust in AI. Moreover, the effect of management commitment and trust in the AI promoter are strengthened when users have high AI self-efficacy. The findings of this study provide suggestions for academics and managers with respect to promoting users’ trust in AI in the manufacturing industry.
Angiopoietins stimulate pancreatic islet development from stem cells
Authors: Karanth Soujanya S. , Sun Shuofei , Bi Huanjing , Ye Kaiming , Jin Sha
Published: June 2021

In vitro differentiation of human induced pluripotent stem cells (iPSCs) into functional islets holds immense potential to create an unlimited source of islets for diabetes research and treatment. A continuous challenge in this field is to generate glucose-responsive mature islets. We herein report a previously undiscovered angiopoietin signal for in vitro islet development. We revealed, for the first time, that angiopoietins, including angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) permit the generation of islets from iPSCs with elevated glucose responsiveness, a hallmark of mature islets. Angiopoietin-stimulated islets exhibited glucose synchronized calcium ion influx in repetitive glucose challenges. Moreover, Ang2 augmented the expression of all islet hormones, including insulin, glucagon, somatostatin, and pancreatic polypeptide; and β cell transcription factors, including NKX6.1, MAFA, UCN3, and PDX1. Furthermore, we showed that the Ang2 stimulated islets were able to regulate insulin exocytosis through actin-filament polymerization and depolymerization upon glucose challenge, presumably through the CDC42-RAC1-gelsolin mediated insulin secretion signaling pathway. We also discovered the formation of endothelium within the islets under Ang2 stimulation. These results strongly suggest that angiopoietin acts as a signaling molecule to endorse in vitro islet development from iPSCs.
Cardiac stress T1-mapping response and extracellular volume stability of MOLLI-based T1-mapping methods
Authors: Burrage Matthew K. , Shanmuganathan Mayooran , Zhang Qiang , Hann Evan , Popescu Iulia A. , Soundarajan Rajkumar , Chow Kelvin , Neubauer Stefan , Ferreira Vanessa M. , Piechnik Stefan K.
Published: June 2021

Stress and rest T1-mapping may assess for myocardial ischemia and extracellular volume (ECV). However, the stress T1 response is method-dependent, and underestimation may lead to misdiagnosis. Further, ECV quantification may be affected by time, as well as the number and dosage of gadolinium (Gd) contrast administered. We compared two commonly available T1-mapping approaches in their stress T1 response and ECV measurement stability. Healthy subjects (n = 10, 50% female, 35 ± 8 years) underwent regadenoson stress CMR (1.5 T) on two separate days. Prototype ShMOLLI 5(1)1(1)1 sequence was used to acquire consecutive mid-ventricular T1-maps at rest, stress and post-Gd contrast to track the T1 time evolution. For comparison, standard MOLLI sequences were used: MOLLI 5(3)3 Low (256 matrix) & High (192 matrix) Heart Rate (HR) to acquire rest and stress T1-maps, and MOLLI 4(1)3(1)2 Low & High HR for post-contrast T1-maps. Stress and rest myocardial blood flow (MBF) maps were acquired after IV Gd contrast (0.05 mmol/kg each). Stress T1 reactivity (delta T1) was defined as the relative percentage increase in native T1 between rest and stress. Myocardial T1 values for delta T1 (dT1) and ECV were calculated. Residuals from the identified time dependencies were used to assess intra-method variability. ShMOLLI achieved a greater stress T1 response compared to MOLLI Low and High HR (peak dT1 = 6.4 ± 1.7% vs. 4.8 ± 1.3% vs. 3.8 ± 1.0%, respectively; both p < 0.0001). ShMOLLI dT1 correlated strongly with stress MBF (r = 0.77, p < 0.001), compared to MOLLI Low HR (r = 0.65, p < 0.01) and MOLLI High HR (r = 0.43, p = 0.07). ShMOLLI ECV was more stable to gadolinium dose with less time drift (0.006–0.04% per minute) than MOLLI variants. Overall, ShMOLLI demonstrated less intra-individual variability than MOLLI variants for stress T1 and ECV quantification. Power calculations indicate up to a fourfold (stress T1) and 7.5-fold (ECV) advantage in sample-size reduction using ShMOLLI. Our results indicate that ShMOLLI correlates strongly with increased MBF during regadenoson stress and achieves a significantly higher stress T1 response, greater effect size, and greater ECV measurement stability compared with the MOLLI variants tested.
Natural variability in air–sea gas transfer efficiency of CO2
Authors: Yang Mingxi , Smyth Timothy J. , Kitidis Vassilis , Brown Ian J. , Wohl Charel , Yelland Margaret J. , Bell Thomas G.
Published: June 2021

The flux of CO2between the atmosphere and the ocean is often estimated as the air–sea gas concentration difference multiplied by the gas transfer velocity (K660). The first order driver forK660over the ocean is wind through its influence on near surface hydrodynamics. However, field observations have shown substantial variability in the wind speed dependencies ofK660. In this study we measuredK660with the eddy covariance technique during a ~ 11,000 km long Southern Ocean transect. In parallel, we made a novel measurement of the gas transfer efficiency (GTE) based on partial equilibration of CO2using a Segmented Flow Coil Equilibrator system. GTE varied by 20% during the transect, was distinct in different water masses, and related toK660. At a moderate wind speed of 7 m s−1,K660associated with high GTE exceededK660with low GTE by 30% in the mean. The sensitivity ofK660towards GTE was stronger at lower wind speeds and weaker at higher wind speeds. Naturally-occurring organics in seawater, some of which are surface active, may be the cause of the variability in GTE and inK660. Neglecting these variations could result in biases in the computed air–sea CO2fluxes.