bluuebunny/crossref_metadata_2025 huggingface dataset:
A Biological-Based Photovoltaic Electrochemical Cell: Modelling the Impedance Spectra
Authors: Alfinito Eleonora , Milano Francesco , Beccaria Matteo , Cataldo Rosella , Giotta Livia , Trotta Massimo , Guascito Maria Rachele
Published: March 2020

The impedance response of an electrochemical cell able to convert sunlight into electrical power is analyzed and discussed. Light conversion is due to a photosynthetic system known as reaction center, which is the core of photosynthesis in several living beings. Under illumination, an abrupt transformation drives the cell electrical response from insulator to conductor and a photocurrent is observed. The impedance spectrum shows a peculiar shape which significantly modifies after the protein activation. It has been analyzed by means of a graphical/analytical/numerical procedure. Some impedance graphical representations are indicated as the most appropriate to suggest the design of an equivalent electrical circuit. Then, the analytical expression of this circuit is formulated and used to set-up a custom Phyton code useful for fitting experimental data. Finally, an appropriate normalization procedure is proposed, which validates data in dark and light and can be useful as a fast screening of measurements.
Using the Maximal Entropy Modeling Approach to Analyze the Evolution of Sedentary Agricultural Societies in Northeast China
Authors: Wachtel Ido , Zidon Royi , Shelach-Lavi Gideon
Published: March 2020

The emergence of agriculture and the evolution of sedentary societies are among the most important processes in human history. However, although archeologists and social scientists have long been studying these processes, our understanding of them is still limited. This article focuses on the Fuxin area in present-day Liaoning province in Northeast China. A systematic archeological survey we conducted in Fuxin in recent years located sites from five successive stages of the evolution of agricultural sedentary society. We used the principles of Maximal Entropy to study changes in settlement patterns during a long-term local trajectory, from the incipient steps toward a sedentary agricultural way of life to the emergence of complex societies. Based on the detailed data collected in the field, we developed a geo-statistical model based on Maximal Entropy (MaxEnt) that characterizes the locational choices of societies during different periods. This combination of high-resolution information on the location and density of archeological remains, along with a maximal entropy-based statistical model, enabled us to chart the long-term trajectory of the interactions between human societies and their natural environment and to better understand the different stages of the transition to developed sedentary agricultural society.
Numerical Investigation on the Thermodynamic Characteristics of a Liquid Film upon Spray Cooling Using an Air-Blast Atomization Nozzle
Authors: Li Jia-Xin , Li Yun-Ze , Li En-Hui , Li Tong
Published: March 2020

This paper developed a three-dimensional model to simulate the process of atomization and liquid film formation during the air-blast spray cooling technological process. The model was solved using the discrete phase model method. Several factors including the thermodynamic characteristics of the liquid film as well as the spray quality with different spray mass flow rates under different spray heights were numerically investigated and discussed. The results show that the varied spray height has little effect on the Sauter Mean Diameter (d32) of the spray droplet, while the thermodynamic characteristics of liquid film including the liquid film height, the liquid film velocity, and the liquid film generation rate are sensitive to the change of the spray height. With the growth of spray mass flow rates, d32, the liquid film generation rate and liquid film height become larger, while the liquid film velocity with different spray mass flow rates has a similar velocity distribution, indicating that the spray mass flow rate has little effect on the liquid film velocity. The average d32 of droplet size shows a sharp drop when sprayed from the nozzle in a short period of time (<1.5 ms), then approaching smoothness, below a value of 40 μ m , the spray status tends to be stable.
Nonlinear Methods Most Applied to Heart-Rate Time Series: A Review
Authors: Henriques Teresa , Ribeiro Maria , Teixeira Andreia , Castro Luísa , Antunes Luís , Costa-Santos Cristina
Published: March 2020

The heart-rate dynamics are one of the most analyzed physiological interactions. Many mathematical methods were proposed to evaluate heart-rate variability. These methods have been successfully applied in research to expand knowledge concerning the cardiovascular dynamics in healthy as well as in pathological conditions. Notwithstanding, they are still far from clinical practice. In this paper, we aim to review the nonlinear methods most used to assess heart-rate dynamics. We focused on methods based on concepts of chaos, fractality, and complexity: Poincaré plot, recurrence plot analysis, fractal dimension (and the correlation dimension), detrended fluctuation analysis, Hurst exponent, Lyapunov exponent entropies (Shannon, conditional, approximate, sample entropy, and multiscale entropy), and symbolic dynamics. We present the description of the methods along with their most notable applications.
Combining Surveys and Sensors to Explore Student Behaviour
Authors: Kontro Inkeri , Génois Mathieu
Published: March 2020

Student belongingness is important for successful study paths, and group work forms an important part of modern university physics education. To study the group dynamics of introductory physics students at the University of Helsinki, we collected network data from seven laboratory course sections of approximately 20 students each for seven consecutive weeks. The data was collected via the SocioPatterns platform, and supplemented with students’ major subject, year of study and gender. We also collected the Mechanics Baseline Test to measure physics knowledge and the Colorado Learning Attitudes about Science Survey to measure attitudes. We developed metrics for studying the small networks of the laboratory sessions by using connections of the teaching assistant as a constant. In the network, we found both demographically homogeneous and heterogeneous groups that are stable. While some students are consistently loosely connected to their networks, we were not able to identify risk factors. Based on our results, the physics laboratory course is equally successful in building strongly connected groups regardless of student demographics in the sections or the formed small groups. SocioPatterns supplemented with surveys thus provides an opportunity to look into the dynamics of students’ social networks.
The Great Displacement: Reading Migration Fiction at the End of the World
Authors: De Bruyn Ben
Published: March 2020

This paper examines how contemporary works of fiction and nonfiction reflect on anticipated cases of climate dislocation. Building on existing research about migrant agency, climate fiction, and human rights, it traces the contours of climate migration discourse before analyzing how three twenty-first-century novels enable us to reimagine the “great displacement” beyond simplistic militarized and humanitarian frames. Zooming in on stories by Mohsin Hamid, John Lanchester, and Margaret Drabble that envision hypothetical calamities while responding to present-day refugee “crises”, this paper explains how these texts interrogate apocalyptic narratives by demilitarizing borderscapes, exploring survivalist mindsets, and interrogating shallow appeals to empathy.
Flow Modeling and Rendering to Support 3D River Shipping Based on Cross-Sectional Observation Data
Authors: Zhang Xuequan , Liu Jin , Hu Zihe , Zhong Ming
Published: March 2020

The flow in meandering rivers is characterized by rapid changes in flow velocity and water level, especially in flooded environments. Accurate cross-sectional observation data enable continuous monitoring of flow conditions, which is important for river navigation. In this paper, cross-sectional data based flow modeling and rendering methods are studied to build an interactive hybrid flow environment for three-dimensional river shipping. First, the sparse cross-sectional data are extrapolated and interpolated to provide dense sampling points. Then, the data are visualized separately by dynamic texture mapping, particle tracking, streamline rendering, and contour surface rendering. Finally, the rendering models are integrated with ship animation to build a comprehensive hybrid river navigation scenario. The proposed methods are tested by visualizing measured cross-sectional data in the Yangtze River using an open-source software, called World Wind. The experimental results demonstrate that the hybrid flow rendering achieves comprehensive visual effect and the rendering frame rate is greater than 30. The interactive hybrid flow visualization is beneficial to support river shipping analysis.
Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998
Authors: Wang Yajuan , Long Deyu , Wang Yanzhen , Wang Changyou , Liu Xinlun , Zhang Hong , Tian Zengrong , Chen Chunhuan , Ji Wanquan
Published: March 2020

Aegilops geniculata Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat (Triticum aestivum) improvement through distant hybridization. In this study, the wheat–Ae. geniculata alien disomic substitution line W16998 was obtained from the BC1F8 progeny of a cross between the common wheat ‘Chinese Spring’ (CS) and Ae. geniculata Roth (serial number: SY159//CS). This line was identified using cytogenetic techniques, analysis of genomic in situ hybridization (GISH), functional molecular markers (Expressed sequence tag-sequence-tagged site (EST–STS) and PCR-based landmark unique gene (PLUG), fluorescence in situ hybridization (FISH), sequential fluorescence in situ hybridization–genomic in situ hybridization (sequential FISH–GISH), and assessment of agronomic traits and powdery mildew resistance. During the anaphase of meiosis, these were evenly distributed on both sides of the equatorial plate, and they exhibited high cytological stability during the meiotic metaphase and anaphase. GISH analysis indicated that W16998 contained a pair of Ae. geniculata alien chromosomes and 40 common wheat chromosomes. One EST–STS marker and seven PLUG marker results showed that the introduced chromosomes of Ae. geniculata belonged to homoeologous group 7. Nullisomic–tetrasomic analyses suggested that the common wheat chromosome, 7A, was absent in W16998. FISH and sequential FISH–GISH analyses confirmed that the introduced Ae. geniculata chromosome was 7Mg. Therefore, W16998 was a wheat–Ae. geniculata 7Mg (7A) alien disomic substitution line. Inoculation of isolate E09 (Blumeria graminis f. sp. tritici) in the seedling stage showed that SY159 and W16998 were resistant to powdery mildew, indeed nearly immune, whereas CS was highly susceptible. Compared to CS, W16998 exhibited increased grain weight and more spikelets, and a greater number of superior agronomic traits. Consequently, W16998 was potentially useful. Germplasms transfer new disease-resistance genes and prominent agronomic traits into common wheat, giving the latter some fine properties for breeding.
Hibernator-Derived Cells Show Superior Protection and Survival in Hypothermia Compared to Non-Hibernator Cells
Authors: Hendriks Koen D.W. , Joschko Christian P. , Hoogstra-Berends Femke , Heegsma Janette , Faber Klaas-Nico , Henning Robert H.
Published: March 2020

Mitochondrial failure is recognized to play an important role in a variety of diseases. We previously showed hibernating species to have cell-autonomous protective mechanisms to resist cellular stress and sustain mitochondrial function. Here, we set out to detail these mitochondrial features of hibernators. We compared two hibernator-derived cell lines (HaK and DDT1MF2) with two non-hibernating cell lines (HEK293 and NRK) during hypothermia (4 °C) and rewarming (37 °C). Although all cell lines showed a strong decrease in oxygen consumption upon cooling, hibernator cells maintained functional mitochondria during hypothermia, without mitochondrial permeability transition pore (mPTP) opening, mitochondrial membrane potential decline or decreased adenosine triphosphate (ATP) levels, which were all observed in both non-hibernator cell lines. In addition, hibernator cells survived hypothermia in the absence of extracellular energy sources, suggesting their use of an endogenous substrate to maintain ATP levels. Moreover, hibernator-derived cells did not accumulate reactive oxygen species (ROS) damage and showed normal cell viability even after 48 h of cold-exposure. In contrast, non-hibernator cells accumulated ROS and showed extensive cell death through ferroptosis. Understanding the mechanisms that hibernators use to sustain mitochondrial activity and counteract damage in hypothermic circumstances may help to define novel preservation techniques with relevance to a variety of fields, such as organ transplantation and cardiac arrest.
Homoarginine- and Creatine-Dependent Gene Regulation in Murine Brains with l-Arginine:Glycine Amidinotransferase Deficiency
Authors: Jensen Märit , Müller Christian , Schwedhelm Edzard , Arunachalam Priyadharshini , Gelderblom Mathias , Magnus Tim , Gerloff Christian , Zeller Tanja , Choe Chi-un
Published: March 2020

l-arginine:glycine amidinotransferase (AGAT) and its metabolites homoarginine (hArg) and creatine have been linked to stroke pathology in both human and mouse studies. However, a comprehensive understanding of the underlying molecular mechanism is lacking. To investigate transcriptional changes in cerebral AGAT metabolism, we applied a transcriptome analysis in brains of wild-type (WT) mice compared to untreated AGAT-deficient (AGAT−/−) mice and AGAT−/− mice with creatine or hArg supplementation. We identified significantly regulated genes between AGAT−/− and WT mice in two independent cohorts of mice which can be linked to amino acid metabolism (Ivd, Lcmt2), creatine metabolism (Slc6a8), cerebral myelination (Bcas1) and neuronal excitability (Kcnip3). While Ivd and Kcnip3 showed regulation by hArg supplementation, Bcas1 and Slc6a8 were creatine dependent. Additional regulated genes such as Pla2g4e and Exd1 need further evaluation of their influence on cerebral function. Experimental stroke models showed a significant regulation of Bcas1 and Slc6a8. Together, these results reveal that AGAT deficiency, hArg and creatine regulate gene expression in the brain, which may be critical in stroke pathology.
Assessing the Effects of Redox Modifier MnTnBuOE-2-PyP 5+ on Cognition and Hippocampal Physiology Following Doxorubicin, Cyclophosphamide, and Paclitaxel Treatment
Authors: McElroy Taylor , Brown Taurean , Kiffer Fred , Wang Jing , Byrum Stephanie D. , Oberley-Deegan Rebecca E. , Allen Antiño R.
Published: March 2020

Background: Chemotherapy treatment for breast cancer can induce cognitive impairments often involving oxidative stress. The brain, as a whole, is susceptible to oxidative stress due to its high-energy requirements, limited anaerobic respiration capacities, and limited antioxidant defenses. The goal of the current study was to determine if the manganese porphyrin superoxide dismutase mimetic MnTnBuOE-2-PyP (MnBuOE) could ameliorate the effects of doxorubicin, cyclophosphamide, and paclitaxel (AC-T) on mature dendrite morphology and cognitive function. Methods: Four-month-old female C57BL/6 mice received intraperitoneal injections of chemotherapy followed by subcutaneous injections of MnBuOE. Four weeks following chemotherapy treatment, mice were tested for hippocampus-dependent cognitive performance in the Morris water maze. After testing, brains were collected for Golgi staining and molecular analyses. Results: MnBuOE treatment preserved spatial memory during the Morris water-maze. MnBuOE/AC-T showed spatial memory retention during all probe trials. AC-T treatment significantly impaired spatial memory retention in the first and third probe trial (no platform). AC-T treatment decreased dendritic length in the Cornu Ammonis 1 (CA1) and dentate gyrus (DG) areas of the hippocampus while AC-T/MnBuOE maintained dendritic length. Comparative proteomic analysis revealed affected protein networks associated with cell morphology and behavior functions in both the AC-T and AC-T/MnBuOE treatment groups.
Differential Abilities of Mammalian Cathelicidins to Inhibit Bacterial Biofilm Formation and Promote Multifaceted Immune Functions of Neutrophils
Authors: Xie Fang , Zan Yanan , Zhang Xinyuan , Zhang Huihui , Jin Mingjie , Zhang Wanjiang , Zhang Yueling , Liu Siguo
Published: March 2020

Mammalian cathelicidins act as the potent microbicidal molecules for controlling bacterial infection, and are considered promising alternatives to traditional antibiotics. Their ability to modulate host immune responses, as well as their bactericidal activities, is essential for therapeutic interventions. In this study, we compared the bactericidal activities, antibiofilm activities and immune-modulatory properties of cathelicidins BMAP-27, BMAP-34, mCRAMP, and LL-37, and evaluated the therapeutic efficacy of the combination of BMAP-27 and LL-37 using a mouse pulmonary infection model. Our results showed that all of the four cathelicidins effectively killed bacteria via rapid induction of membrane permeabilization, and BMAP-27 exhibited the most excellent bactericidal activity against diverse bacterial pathogens. BMAP-27, mCRAMP, and LL-37 effectively inhibited biofilm formation, while BMAP-34, mCRAMP and LL-37 exerted immunomodulatory functions with varying degrees of efficacy by stimulating the chemotaxis of neutrophils, inducing the production of reactive oxygen species, and facilitating the formation of neutrophil extracellular traps. Of note, the combination of BMAP-27 and LL-37 effectively enhanced the clearance of Pseudomonas aeruginosa and reduced the organ injury in vivo. Together, these findings highlight that identifying the appropriate synergistic combination of mammalian cathelicidins with different beneficial properties may be an effective strategy against bacterial infection.
Comparative Analysis of Complete Mitochondrial Genomes of Three Gerres Fishes (Perciformes: Gerreidae) and Primary Exploration of Their Evolution History
Authors: Ruan Huiting , Li Min , Li Zhenhai , Huang Jiajie , Chen Weiyuan , Sun Jijia , Liu Li , Zou Keshu
Published: March 2020

Mitochondrial genome is a powerful molecule marker to explore phylogenetic relationships and reveal molecular evolution in ichthyological studies. Gerres species play significant roles in marine fishery, but its evolution has received little attention. To date, only two Gerres mitochondrial genomes were reported. In the present study, three mitogenomes of Gerres (Gerres filamentosus, Gerres erythrourus, and Gerres decacanthus) were systemically investigated. The lengths of the mitogenome sequences were 16,673, 16,728, and 16,871 bp for G. filamentosus, G. erythrourus, and G. decacanthus, respectively. Most protein-coding genes (PCGs) were initiated with the typical ATG codon and terminated with the TAA codon, and the incomplete termination codon T/TA could be detected in the three species. The majority of AT-skew and GC-skew values of the 13 PCGs among the three species were negative, and the amplitude of the GC-skew was larger than the AT-skew. The genetic distance and Ka/Ks ratio analyses indicated 13 PCGs were suffering purifying selection and the selection pressures were different from certain deep-sea fishes, were which most likely due to the difference in their living environment. The phylogenetic tree was constructed by molecular method (Bayesian Inference (BI) and maximum Likelihood (ML)), providing further supplement to the scientific classification of fish. Three Gerres species were differentiated in late Cretaceous and early Paleogene, and their evolution might link with the geological events that could change their survival environment.
Major Latex Protein MdMLP423 Negatively Regulates Defense against Fungal Infections in Apple
Authors: He Shanshan , Yuan Gaopeng , Bian Shuxun , Han Xiaolei , Liu Kai , Cong Peihua , Zhang Caixia
Published: March 2020

Major latex proteins (MLPs) play critical roles in plants defense and stress responses. However, the roles of MLPs from apple (Malus × domestica) have not been clearly identified. In this study, we focused on the biological role of MdMLP423, which had been previously characterized as a potential pathogenesis-related gene. Phylogenetic analysis and conserved domain analysis indicated that MdMLP423 is a protein with a ‘Gly-rich loop’ (GXGGXG) domain belonging to the Bet v_1 subfamily. Gene expression profiles showed that MdMLP423 is mainly expressed in flowers. In addition, the expression of MdMLP423 was significantly inhibited by Botryosphaeria berengeriana f. sp. piricola (BB) and Alternaria alternata apple pathotype (AAAP) infections. Apple calli overexpressing MdMLP423 had lower expression of resistance-related genes, and were more sensitive to infection with BB and AAAP compared with non-transgenic calli. RNA-seq analysis of MdMLP423-overexpressing calli and non-transgenic calli indicated that MdMLP423 regulated the expression of a number of differentially expressed genes (DEGs) and transcription factors, including genes involved in phytohormone signaling pathways, cell wall reinforcement, and genes encoding the defense-related proteins, AP2-EREBP, WRKY, MYB, NAC, Zinc finger protein, and ABI3. Taken together, our results demonstrate that MdMLP423 negatively regulates apple resistance to BB and AAAP infections by inhibiting the expression of defense- and stress-related genes and transcription factors.
Bacteriophage-Insensitive Mutants of Antimicrobial-Resistant Salmonella Enterica are Altered in their Tetracycline Resistance and Virulence in Caco-2 Intestinal Cells
Authors: Fong Karen , Mu Kaiwen , Rheault Jean-Guillaume , Levesque Roger C. , Kitts David D. , Delaquis Pascal , Goodridge Lawrence , Wang Siyun
Published: March 2020

Bacteriophages have shown promise as therapeutic alternatives to antibiotics for the control of infectious bacteria, including the human pathogen Salmonella. However, the development of effective phage-based applications requires the elucidation of key interactions between phages and target hosts, particularly since host resistance to phage is inevitable. Little is known about the alteration of host phenotypes following the development of resistance to phage. The aim of this study is to evaluate the antibiotic susceptibility and virulence of a Salmonella isolate following the development of resistance to bacteriophage SI1. We observed enhanced susceptibility to tetracycline and decreased invasion capacity in a differentiated Caco-2 intestinal cell line. Whole genome sequence analysis revealed an array of mutations, most notably, truncations in vgrG1_2, a core gene involved in Type VI secretion and mutations in the lipopolysaccharide, thereby indicating the plausible attachment site of phage SI1. These findings shed light on understanding the underlying mechanism for phage immunity within the host. Importantly, we reveal an associated genetic cost to the bacterial host with developing resistance to phages. Taken together, these results will aid in advancing strategies to delay or eliminate the development of host resistance when designing informed phage-based antimicrobials.
Promoting Roles of Embryonic Signals in Embryo Implantation and Placentation in Cooperation with Endocrine and Immune Systems
Authors: Fujiwara Hiroshi , Ono Masanori , Sato Yukiyasu , Imakawa Kazuhiko , Iizuka Takashi , Kagami Kyosuke , Fujiwara Tomoko , Horie Akihito , Tani Hirohiko , Hattori Akira , Daikoku Takiko , Araki Yoshihiko
Published: March 2020

Embryo implantation in the uterus is an essential process for successful pregnancy in mammals. In general, the endocrine system induces sufficient embryo receptivity in the endometrium, where adhesion-promoting molecules increase and adhesion-inhibitory molecules decrease. Although the precise mechanisms remain unknown, it is widely accepted that maternal–embryo communications, including embryonic signals, improve the receptive ability of the sex steroid hormone-primed endometrium. The embryo may utilize repulsive forces produced by an Eph–ephrin system for its timely attachment to and subsequent invasion through the endometrial epithelial layer. Importantly, the embryonic signals are considered to act on maternal immune cells to induce immune tolerance. They also elicit local inflammation that promotes endometrial differentiation and maternal tissue remodeling during embryo implantation and placentation. Additional clarification of the immune control mechanisms by embryonic signals, such as human chorionic gonadotropin, pre-implantation factor, zona pellucida degradation products, and laeverin, will aid in the further development of immunotherapy to minimize implantation failure in the future.
Epigenetic Regulation of WNT3A Enhancer during Regeneration of Injured Cortical Neurons
Authors: Chang Chu-Yuan , Hung Jui-Hung , Huang Liang-Wei , Li Joye , Fung Ka Shing , Kao Cheng-Fu , Chen Linyi
Published: March 2020

Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration.
Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat (Avena sativa L.) Seeds
Authors: Yan Huifang , Jia Shangang , Mao Peisheng
Published: March 2020

Although melatonin has been reported to play an important role in regulating metabolic events under adverse stresses, its underlying mechanisms on germination in aged seeds remain unclear. This study was conducted to investigate the effect of melatonin priming (MP) on embryos of aged oat seeds in relation to germination, ultrastructural changes, antioxidant responses, and protein profiles. Proteomic analysis revealed, in total, 402 differentially expressed proteins (DEPs) in normal, aged, and aged + MP embryos. The downregulated DEPs in aged embryos were enriched in sucrose metabolism, glycolysis, β-oxidation of lipid, and protein synthesis. MP (200 μM) turned four downregulated DEPs into upregulated DEPs, among which, especially 3-ketoacyl-CoA thiolase-like protein (KATLP) involved in the β-oxidation pathway played a key role in maintaining TCA cycle stability and providing more energy for protein translation. Furthermore, it was found that MP enhanced antioxidant capacity in the ascorbate-glutathione (AsA-GSH) system, declined reactive oxygen species (ROS), and improved cell ultrastructure. These results indicated that the impaired germination and seedling growth of aged seeds could be rescued to a certain level by melatonin, predominantly depending on β-oxidation, protein translation, and antioxidant protection of AsA-GSH. This work reveals new insights into melatonin-mediated mechanisms from protein profiles that occur in embryos of oat seeds processed by both aging and priming.
Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality
Authors: Mańkowska Anna , Brym Paweł , Paukszto Łukasz , Jastrzębski Jan P. , Fraser Leyland
Published: March 2020

Genetic markers have been used to assess the freezability of semen. With the advancement in molecular genetic techniques, it is possible to assess the relationships between sperm functions and gene polymorphisms. In this study, variant calling analysis of RNA-Seq datasets was used to identify single nucleotide polymorphisms (SNPs) in boar spermatozoa and to explore the associations between SNPs and post-thaw semen quality. Assessment of post-thaw sperm quality characteristics showed that 21 boars were considered as having good semen freezability (GSF), while 19 boars were classified as having poor semen freezability (PSF). Variant calling demonstrated that most of the polymorphisms (67%) detected in boar spermatozoa were at the 3’-untranslated regions (3’-UTRs). Analysis of SNP abundance in various functional gene categories showed that gene ontology (GO) terms were related to response to stress, motility, metabolism, reproduction, and embryo development. Genomic DNA was isolated from sperm samples of 40 boars. Forty SNPs were selected and genotyped, and several SNPs were significantly associated with motility and membrane integrity of frozen-thawed (FT) spermatozoa. Polymorphism in SCLT1 gene was associated with significantly higher motility and plasma membrane integrity of FT spermatozoa from boars of the GSF group compared with those of the PSF group. Likewise, polymorphisms in MAP3K20, MS4A2, and ROBO1 genes were significantly associated with reduced cryo-induced lipid peroxidation and DNA damage of FT spermatozoa from boars of the GSF group. Candidate genes with significant SNP associations, including APPL1, PLBD1, FBXO16, EML5, RAB3C, OXSR1, PRICKLE1, and MAP3K20 genes, represent potential markers for post-thaw semen quality, and they might be relevant for future improvement in the selection procedure of boars for cryopreservation. The findings of this study provide evidence indicating that polymorphisms in genes expressed in spermatozoa could be considered as factors associated with post-thaw semen quality.
Exposure to Dichlorodiphenyldichloroethylene (DDE) and Metallothionein Levels in Rats Fed with Normocaloric or High-Fat Diet: A Review
Authors: Migliaccio Vincenzo , Lionetti Lillà , Putti Rosalba , Scudiero Rosaria
Published: March 2020

The growing number of studies on metallothioneins (MTs), cysteine-rich metal-binding proteins, have been disclosing new functions of these proteins. Thanks to their inducibility, they were considered to play a pivotal role in regulating trace metals homeostasis and in detoxification from heavy metals; nowadays, it is known that they are involved in various physiological and pathological processes, such as regulation of apoptosis, elimination of free radicals, and protection of nucleic acids against toxic insults. MT induction has been demonstrated following stress factors other than heavy metals, such as endocrine-disrupting chemicals, insecticides, and herbicides. However, retrieved data are often controversial: in some cases, xenobiotics elicit MT expression and synthesis; under different conditions, they lead to a decrease in cellular MT content. This review describes the MT response to dichlorodiphenyltrichloroethane (DDT) contamination in mammalian tissues. In particular, attention focuses on changes in MT expression, synthesis, and localization in rat liver, kidneys, and testes following oral administration of dichlorodiphenyldichloroethylene (DDE), the main metabolite of DDT, under normal dietary conditions or in combination with a high fat diet potentially able to increase the cellular uptake of this lipophilic pesticide. The potential connection between MT expression and synthesis, lipophilic substances and trace metals availability is also discussed.
T-Coin: Dynamic Traffic Congestion Pricing System for the Internet of Vehicles in Smart Cities
Authors: Aung Nyothiri , Zhang Weidong , Dhelim Sahraoui , Ai Yibo
Published: March 2020

Alleviating traffic congestion is one of the main challenges for the Internet of Vehicles (IoV) in smart cities. Many congestion pricing systems have been proposed recently. However, most of them focus on punishing the vehicles that use certain roads during peak hours, neglecting the proven fact that rewards can encourage drivers to follow the rules. Therefore, in this paper, we propose a new congestion pricing system based on reward and punishment policies for the IoV in a smart city environment, where the vehicles are rewarded for voluntarily choosing to take an alternative path to alleviate traffic congestion. The proposed system is implemented using vehicular ad hoc networks, which eliminate the need for installing a costly electronic toll collection system. We propose a new virtual currency called T-Coin (traffic coin), that is used to reward the vehicles for their positive attitude. T-Coin is also used in the tender between vehicles to manage the road reservation process. The proposed system uses dynamic pricing to adapt to peak-hour traffic congestion. Using simulated traffic on a real map of Beijing city, we prove the usefulness of T-Coin as a traffic congestion pricing system.
Using the 16PF to Test the Differentiation of Personality by Intelligence Hypothesis
Authors: Schermer Julie Aitken , Krammer Georg , Goffin Richard D. , Biderman Michael D.
Published: March 2020

The differentiation of personality by intelligence hypothesis suggests that there will be greater individual differences in personality traits for those individuals who are more intelligent. Conversely, less intelligent individuals will be more similar to each other in their personality traits. The hypothesis was tested with a large sample of managerial job candidates who completed an omnibus personality measure with 16 scales and five intelligence measures (used to generate an intelligence g-factor). Based on the g-factor composite, the sample was split using the median to conduct factor analyses within each half. A five-factor model was tested for both the lower and higher intelligence halves and were found to have configural invariance but not metric or scalar invariance. In general, the results provide little support for the differentiation hypothesis as there was no clear and consistent pattern of lower inter-scale correlations for the more intelligent individuals.
Advances in Understanding the Acyl-CoA-Binding Protein in Plants, Mammals, Yeast, and Filamentous Fungi
Authors: Qiu Shangkun , Zeng Bin
Published: March 2020

Acyl-CoA-binding protein (ACBP) is an important protein with a size of about 10 kDa. It has a high binding affinity for C12–C22 acyl-CoA esters and participates in lipid metabolism. ACBP and its family of proteins have been found in all eukaryotes and some prokaryotes. Studies have described the function and structure of ACBP family proteins in mammals (such as humans and mice), plants (such as Oryza sativa, Arabidopsis thaliana, and Hevea brasiliensis) and yeast. However, little information on the structure and function of the proteins in filamentous fungi has been reported. This article concentrates on recent advances in the research of the ACBP family proteins in plants and mammals, especially in yeast, filamentous fungi (such as Monascus ruber and Aspergillus oryzae), and fungal pathogens (Aspergillus flavus, Cryptococcus neoformans). Furthermore, we discuss some problems in the field, summarize the binding characteristics of the ACBP family proteins in filamentous fungi and yeast, and consider the future of ACBP development.
The Potential Supply and Demand of Farmers’ Land Contract Rights-Based on 697 Households in Four Provinces of China
Authors: Wang Wujing , Ye Xingqing
Published: March 2020

A new urbanization and rural revitalization strategy has been implemented in China over a number of years, under which farmers’ land contract rights (LCRs) flow inevitably through various means. The practice in reform pilot areas indicates that government funds cannot meet all the needs, so exploring market-based LCR payout paths is important for rural land tenure system reform. The purpose of this study is to answer questions such as the following: How would farmers respond if they were allowed to trade LCRs? Is there an equilibrium point between the potential supply and demand of LCRs? Which factors would affect the potential supply and demand of LCRs? In this study, 697 valid questionnaires from Ningxia, Hebei, Henan, and Shandong provinces, China, were used for analysis by the multiple bounded discrete choice (MBDC) method and MBDC-Tobit model. The results show that there is a potential market among rural collective households in China, with an equilibrium price of ¥27,800/mu ($59,714.4/ha), and a proportion of farmers who are willing to buy or sell LCRs is around 10.0%. The factors affecting the potential supply and demand of LCRs include land grade, average agricultural income per unit, total money to buy urban houses and cars, age, number of household members with a college education or above, and risk appetite. If the institutional barriers that hinder LCR transactions were eliminated, the potential supply and demand of LCRs would be matched, and the market would provide funds for next-stage reforms.
Effect of PBO–FRCM Reinforcement on Stiffness of Eccentrically Compressed Reinforced Concrete Columns
Authors: Trapko Tomasz , Musiał Michał
Published: March 2020

This paper examines the effect of PBO (P-phenylene benzobisoxazole)–FRCM (Fabric Reinforced Cementitious Matrix) reinforcement on the stiffness of eccentrically compressed reinforced concrete columns. Reinforcement with FRCM consists of bonding composite meshes to the concrete substrate by means of mineral mortar. Longitudinal and/or transverse reinforcements made of PBO (P-phenylene benzobisoxazole) mesh were applied to the analyzed column specimens. When assessing the stiffness of the columns, the focus was on the effect of the composite reinforcement itself, the value and eccentricity of the longitudinal force and the decrease in the modulus of elasticity of the concrete with increasing stress intensity in the latter. Dependences between the change in the elasticity modulus of the concrete and the change in the stiffness of the tested specimens were examined. The relevant standards, providing methods of calculating the stiffness of composite columns, were used in the analysis. For columns, which were strengthened only transversely with PBO mesh, reinforcement increases their load capacity, and at the same time, the stiffness of the columns increases due to the confinement of the cross-section. The stiffness depends on the destruction of the concrete core inside its composite jacket. In the case of columns with transverse and longitudinal reinforcement, the presence of longitudinal reinforcement reduces longitudinal deformations. The columns failed at higher stiffness values in the whole range of the eccentricities.
Regeneration Mechanism of Sulfur Absorption Via Samarium-doped Cerium Adsorbents in the Gas Atmosphere of O2/N2
Authors: Hu Xuechao , Dong Junhui
Published: March 2020

Sulfides existing in many high-temperature gas mixtures have a negative effect on various industrial applications. Ce-based adsorbents are becoming a hotspot in the high-temperature desulfurization process owing to their excellent thermal stability at high temperatures and regeneration capacity. In this study, we investigate the regeneration path of samarium-doped cerium (SDC) sorbent at high temperature. The SDC adsorbent showed a good sulfur removal ability and excellent regeneration capacity. Ce2O2S and Ce(SO4)2 are observed in the used adsorbent, and Ce2O2S is the main sulfur-containing species. The regeneration path of the Ce2O2S is the key to the regeneration mechanism of the adsorbent. There are two regeneration paths for the Ce2O2S at high temperature in O2/N2 gas mixture. In air stream, the Ce2O2S is oxidized to Ce2O2SO4 and then decomposes into CeO2 and SO2. In a 2% O2/N2 gas condition, the Ce2O2S directly generates CeO2 and elemental sulfur with O2 assistance.
Wash Testing of Electronic Yarn
Authors: Hardy Dorothy Anne , Rahemtulla Zahra , Satharasinghe Achala , Shahidi Arash , Oliveira Carlos , Anastasopoulos Ioannis , Nashed Mohamad Nour , Kgatuke Matholo , Komolafe Abiodun , Torah Russel , Tudor John , Hughes-Riley Theodore , Beeby Steve , Dias Tilak
Published: March 2020

Electronically active yarn (E-yarn) pioneered by the Advanced Textiles Research Group of Nottingham Trent University contains a fine conductive copper wire soldered onto a package die, micro-electro-mechanical systems device or flexible circuit. The die or circuit is then held within a protective polymer packaging (micro-pod) and the ensemble is inserted into a textile sheath, forming a flexible yarn with electronic functionality such as sensing or illumination. It is vital to be able to wash E-yarns, so that the textiles into which they are incorporated can be treated as normal consumer products. The wash durability of E-yarns is summarized in this publication. Wash tests followed a modified version of BS EN ISO 6330:2012 procedure 4N. It was observed that E-yarns containing only a fine multi-strand copper wire survived 25 cycles of machine washing and line drying; and between 5 and 15 cycles of machine washing followed by tumble-drying. Four out of five temperature sensing E-yarns (crafted with thermistors) and single pairs of LEDs within E-yarns functioned correctly after 25 cycles of machine washing and line drying. E-yarns that required larger micro-pods (i.e., 4 mm diameter or 9 mm length) were less resilient to washing. Only one out of five acoustic sensing E-yarns (4 mm diameter micro-pod) operated correctly after 20 cycles of washing with either line drying or tumble-drying. Creating an E-yarn with an embedded flexible circuit populated with components also required a relatively large micro-pod (diameter 0.93 mm, length 9.23 mm). Only one embedded circuit functioned after 25 cycles of washing and line drying. The tests showed that E-yarns are suitable for inclusion in textiles that require washing, with some limitations when larger micro-pods were used. Reduction in the circuit’s size and therefore the size of the micro-pod, may increase wash resilience.
The Oxidation Resistance of Nb-Si-Based Alloys at Intermediate and High Temperatures
Authors: Yang Jianyong , Zhuo Guanqun , Jiang Kaiyong , Zhu Xinghan , Su Linfen
Published: March 2020

The oxidation behavior of three Nb-Si-based alloys were evaluated at intermediate (800 °C) and high (1250 °C) temperatures for 100 h in air. At 800 °C, the Nb-24Ti-15Si-13Cr-2Al-2Hf (at. %) alloy suffered from catastrophic pest oxidation. This pest phenomenon was suppressed by the addition of Sn. However, Ta addition protected the Nb-Si-based alloys from pest oxidation for a short time. At 1250 °C, Sn could enhance the oxidation resistance of Nb-Si-based alloys due to the formation of a Sn-rich layer. In addition, the oxidation mechanisms of Nb-Si-based alloys at intermediate and high temperatures were discussed.
Tribological Performance of Micro-Groove Tools of Improving Tool Wear Resistance in Turning AISI 304 Process
Authors: Wu Jinxing , Zhan Gang , He Lin , Zou Zhongfei , Zhou Tao , Du Feilong
Published: March 2020

AISI 304 has good physical and chemical properties and thus is widely used. However, due to the low thermal diffusivity, the cutting temperature of AISI 304 is high accelerating the wear of the tool. Therefore, tool wear is a major problem in machining hard cutting materials. In this study, we developed a new type of micro-groove tool whose rake surface was distributed with micro-groove by powder metallurgy based on the finite element temperature field morphology. We compared the wear of the proposed micro-groove tool with an untreated one by using a scanning electron microscope (SEM) and an X-ray energy spectrum. The abrasive, adhesive, and oxidation wear of the rake and the flank face of the micro-groove tool were lower than that of the untreated one. Due to the micro-groove on the rake face of the tool, the contact length between the tool and chip was reduced, leaving more extension space. Furthermore, chip extrusion deformation was avoided, and the energy caused by chip deformation was reduced. After 70 min of cutting, the counterpart reached the specified wear amount while the main cutting force, the feed resistance, and the cutting depth resistance of the proposed micro-groove tool were reduced by 16.1%, 33.9%, and 40.1%, respectively. With regard to steady state, the cutting temperature was reduced by 17.2% and the wear width of the flank face was reduced by 36.7%.
Hydraulic Activity and Microstructure Analysis of High-Titanium Slag
Authors: Hou Xinkai , Wang Dan , Shi Yiming , Guo Haitao , He Yingying
Published: March 2020

To explain the relationship between the hydration activity of high-titanium slag and its microstructure, the hydration activity of high-titanium slag was determined, then the mineral phase and microstructure characteristics of high-titanium slag glass phase and blast furnace slag were investigated using a series of analytical methods, which contain X-Ray Diffraction (XRD), Scanning Electronic Microscope (SEM), Fourier Transform Infrared spectroscopy (FTIR), Raman spectroscopy, and Nuclear Magnetic Resonance spectroscopy (NMR). The results showed that in slow-cooled high-titanium slag, the hydration inert mineral content was about 98%, and the glass phase content was less than 2%, hence, the hydration activity of slow-cooled high titanium slag accounted for less than 25% of that of the blast furnace slag. The content of the glass phase in water-quenched high-titanium slag was 98%, but the microstructure of the glass phase was very different from that of the blast furnace slag. The glass phase of high-titanium slag has stable forms, which are TiO44− monomers, chain or sheet units O–Ti–O, and a small amount of 6-coordination Ti4+. The Ti makes the SiO4 tetrahedron in a glass phase network not only a monosilicate, but more stable forms of disilicates and chain middle groups also appeared. The relative bridge oxygen number increased to 0.2, hence, the hydration activity of water-quenched high-titanium slag took up less than 37% of that of the blast furnace slag.