bluuebunny/crossref_metadata_2025 huggingface dataset:
Physical Preparation in Female Rugby Codes: An Investigation of Current Practices
Authors: Heyward Omar , Nicholson Ben , Emmonds Stacey , Roe Gregory , Jones Ben
Published: November 2020

Female sports have recently seen a dramatic rise in participation and professionalism world-wide. Despite progress, the infrastructure and general sport science provisions in many female sports are behind their male counterparts. From a performance perspective, marked differences in physical and physiological characteristics can be seen between the sexes. Although physical preparation practices for male athletes are known, there are currently no published literature pertaining exclusively to female athletes. This information would provide invaluable data for both the researcher and practitioner alike. This survey therefore aimed to examine current practices utilized in female rugby codes (union, league, and sevens). A questionnaire assessing seasonal physical preparation practices, recovery, monitoring and sport science technology, and unique aspects in female rugby was developed. Thirty-seven physical preparation practitioners (32 males, 5 females) responded to the questionnaire. Most participants (78%) worked with national or regional/state level female athletes. Performance testing was more frequently assessed in the pre- (97%) and in-season (86%), than off-season (23%). Resistance, cardiovascular, sprint and plyometric training, and recovery sessions were all believed to be important to enhancing performance and implemented by most participants (≥ 89%). Sport science technologies were commonly (54%) utilized to inform current practice. Menstrual cycle phase was monitored by 22% of practitioners. The most frequently reported unique considerations in female rugby codes included psycho-social aspects (41%), the menstrual cycle (22%), and physical differences (22%). Practitioners working with female rugby can use the presented data to inform and develop current practices.
Complete Osseointegration of a Retrieved 3-D Printed Porous Titanium Cervical Cage
Authors: van den Brink Wimar , Lamerigts Nancy
Published: November 2020

Introduction: Porous 3D-printed titanium has only recently been introduced for spinal applications. Evidence around its use is currently limited to animal studies and only few human case series. This study describes the histological findings of a retrieved EIT cervical cage, explanted 2 years after insertion.

Materials and Methods: The patient underwent a double level C4/C5 & C5/C6 anterior cervical decompression using EIT cervical cages without an anterior plate. Two years later the C6/7 level degenerated and began to cause myelopathic symptoms. In order to address the kyphotic imbalance of the cervical spine and fix the C6/7 level, the surgeon decided to remove the C5/6 cervical cage and bridge the fusion from C4 to C7 inclusive. The retrieved cage was histologically evaluated for bone ingrowth and signs of inflammation.

Results: MRI demonstrated spinal canal stenosis at C6/C7. Plain radiographs confirmed well-integrated cervical cages at 2 years postoperative. The peroperative surgical need to use a chisel to remove the implant at C5/C6 reconfirmed the solid fusion of the segment. Macroscopically white tissue, indicative of bone, was present at both superior and inferior surfaces of the explanted specimen. Histological evaluation revealed complete osseointegration of the 5 mm high EIT Cellular Titanium® cervical cage, displaying mature lamellar bone in combination with bone marrow throughout the cage. Furthermore, a pattern of trabecular bone apposition (without fibrous tissue interface) and physiological remodeling activity was observed directly on the cellular titanium scaffold.

Conclusion: This histological retrieval study of a radiologically fused cervical EIT cage clearly demonstrates complete osseointegration within a 2-year time frame. The scaffold exhibits a bone in growth pattern and maturation of bone tissue similar of what has been demonstrated in animal studies evaluating similar porous titanium implants. The complete osseointegration throughout the cage indicates physiological loading conditions even in the central part of the cage. This pattern suggests the absence, or at least the minimization, of stress-shielding in this type of porous titanium cage.
Workaholism in IT: An Analysis of the Influence Factors
Authors: Dospinescu Octavian , Dospinescu Nicoleta
Published: November 2020

The purpose of this article is to make an exhaustive analysis of the influencing factors for the phenomenon of workaholism in IT. In contemporary economies, workaholism is an increasingly common phenomenon. Companies should evaluate the impact on workers of this trend, and note that knowledge of the factors influencing the decision to overwork can be a competitive advantage when implementing human resources policies. Our approach involved the use of multivariate analysis of variance combined with multiple linear regression analysis. The article analyzes the data from 178 respondents who are employed in the IT sector in Romania, taking into account seven potential factors of influence. The data and econometric analyses were processed in IBM SPSS Software. The results of the multivariate analysis of variance combined with multiple linear regression analysis show a significant relationship between workaholism and influencing factors such as the remunerative component of work, the intrinsic pleasure of working, the sense of responsibility towards family, and the desire to develop during a professional career. In this study, socio-demographic influences were found to have a weak influence on the decision to work excessively.
Study on Anti-Friction Mechanism of Canna-Leaf Biomimetic Micro-Textured Fruit Tree
Authors: Sun Jianfeng , Li Bo , Xing Kaifeng , Liu Zhu , Yang Zhou
Published: November 2020

Fruit tree pruning is an important part of orchard management. In this paper, the force on and the wear of the pruner in the pruning process were studied with a canna-leaf biomimetic convex-hull pruner. The pruner was formed by laser etching technology. The influence of laser power and scanning speed on the geometric dimensioning of the micro texture was analyzed. The shear force calculation model was built to obtain the positive pressure load during the pruning process, while the model accuracy was verified in the static pressure shear test, and the wear mechanism was analyzed in the wear test. The real pruning process was simulated to compare the worn areas of the textured and non-textured pruners and the number of cuts in fixed wear condition, for proving the wear reduction characteristics of the micro-textured pruner. The results show that: the optimal forming parameters are 70 W 1.6 mm/s (10 mm-diameter branches), 80 W 2.4 mm/s (15 mm-diameter branches) and 80 W 1.6 mm/s (20 mm-diameter branches), and the convex hull spacing is 300 μm. Laser power affects the depth and width of the texture, while scanning speed affects the depth of the texture. The positive pressure on the pruner is proportional to the modulus of elasticity, moment of inertia, cut depth, and bevel angle, whilst it is inversely proportional to the distance from the fixed point of the blade to the positive pressure. The wear test shows that the anti-wear performance of the textured pruner is not obvious at the load of 300 g, while the anti-wear performance of the textured pruner is significant at the loads of 1000 g and 2000 g. The wear mechanism shifts from the abrasive wear in the early stage to more complex oxidative wear and adhesive wear. The actual shear test shows that the textured pruner wears less than the non-textured pruner and enters the stable shear faster.
Genome-Wide DNA Methylation Changes of Perirenal Adipose Tissue in Rabbits Fed a High-Fat Diet
Authors: Shao Jiahao , Bai Xue , Pan Ting , Li Yanhong , Jia Xianbo , Wang Jie , Lai Songjia
Published: November 2020

DNA methylation is an epigenetic mechanism that plays an important role in gene regulation without an altered DNA sequence. Previous studies have demonstrated that diet affects obesity by partially mediating DNA methylation. Our study investigated the genome-wide DNA methylation of perirenal adipose tissue in rabbits to identify the epigenetic changes of high-fat diet-mediated obesity. Two libraries were constructed pooling DNA of rabbits fed a standard normal diet (SND) and DNA of rabbits fed a high-fat diet (HFD). Differentially methylated regions (DMRs) were identified using the option of the sliding window method, and online software DAVID Bioinformatics Resources 6.7 was used to perform Gene Ontology (GO) terms and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis of DMRs-associated genes. A total of 12,230 DMRs were obtained, of which 2305 (1207 up-regulated, 1098 down-regulated) and 601 (368 up-regulated, 233 down-regulated) of identified DMRs were observed in the gene body and promoter regions, respectively. GO analysis revealed that the DMRs-associated genes were involved in developmental process (GO:0032502), cell differentiation (GO:0030154), and lipid binding (GO:0008289), and KEGG pathway enrichment analysis revealed the DMRs-associated genes were enriched in linoleic acid metabolism (KO00591), DNA replication (KO03030), and MAPK signaling pathway (KO04010). Our study further elucidates the possible functions of DMRs-associated genes in rabbit adipogenesis, contributing to the understanding of HFD-mediated obesity.
Social Context Influences Resting Physiology in Dogs
Authors: Kortekaas Kim , Kotrschal Kurt
Published: November 2020

Domestication has affected the social life of dogs. They seem to be less dependent on their pack members than wolves, potentially causing dogs to be more alert towards their environment, especially when resting. Such a response has been found in dogs resting alone compared to wolves in the same situation. However, as this may be influenced by social context, we compared alertness (i.e., degree of activation along the sleep–wake continuum—measured via cardiac parameters) of pack-living and enclosure-kept dogs in two conditions: (1) alone, and (2) with pack members, and in two states of activation: (1) inactive wakefulness, and (2) resting. We found that when dogs were resting alone, alertness was higher than when resting in the pack; individual alertness was potentially influenced by social rank. However, alertness was similar in the two conditions during inactive wakefulness. Thus, depending on social context, familiar conspecifics may still provide support in dogs; i.e., domestication has probably only partly shifted the social orientation of dogs from conspecifics to humans. We suggest that cardiac responses of dogs may be more flexible than those of wolves because of their adaptation to the more variable presence of humans and conspecifics in their environment.
A Comparison of the Quality of Meat from Female and Male Californian and Flemish Giant Gray Rabbits
Authors: Daszkiewicz Tomasz , Gugołek Andrzej
Published: November 2020

This study aimed to compare the quality of meat (Longissimus thoracis et lumborum—LTL, hind legs—HL) from female and male Californian (CAL) and Flemish Giant Gray (FG) rabbits. The animals were kept outdoor, in a roofed pavilion, in wire mesh cages with a slatted floor and were fed ad libitum a complete pelleted diet. All rabbits were slaughtered at 91 days of age. The meat of CAL rabbits had a higher content of dry matter (p < 0.001), protein (p < 0.001) and unsaturated fatty acids (p = 0.002), higher pH (p < 0.001), and higher taste desirability (p = 0.021) and tenderness (p = 0.046). CAL rabbit meat had also a lower (p < 0.001) water to protein (W/P) ratio, lower (p < 0.001) shear force values, and lower (p = 0.042) taste intensity. The meat of FG rabbits was characterized by lower (p < 0.001) water-holding capacity (WHC). The LTL muscle had a higher content of dry matter (p = 0.003) and protein (p < 0.001), higher L* (lightness) (p = 0.011), b* (yellowness) (p < 0.001), and C* (chroma) (p = 0.010) values, and lower (p = 0.015) WHC. Hind leg muscles had higher (p < 0.001) fat content, a higher (p < 0.001) W/P ratio, and pH (p < 0.001). Sex had no effect (p > 0.05) on the analyzed meat quality attributes, excluding vitamin A content which was higher (p = 0.041) in females. In conclusion, the meat of CAL rabbits slaughtered at 91 days of age can be more mature than the meat of FG rabbits slaughtered at the same age. Furthermore, quality of meat from rabbits of both breeds depends on the anatomical origin of muscles. Further research is needed to identify the possible reasons for the higher vitamin A content of meat from female rabbits which did not differ from the meat of males in terms of other characteristics.
Investigation of Different Storage Systems for Solar-Driven Organic Rankine Cycle
Authors: Bellos Evangelos , Sarakatsanis Ioannis , Tzivanidis Christos
Published: November 2020

The objective of the present work is the study of different thermal storage systems for a solar-fed organic Rankine cycle (ORC) system that operates with parabolic trough collectors. The conventional design with sensible thermal oil storage is compared with a storage configuration with thermal oil and ceramic rocks, as well as the use of latent storage with phase change materials (PCMs) is investigated. The initial system is studied parametrically, and it is properly designed to order for the cycle to have high performance. Different organic fluids are studied in the organic Rankine cycle and different rocks are investigated as storage materials. Toluene is found to be the best candidate in the cycle and ceramic rocks are found to be the best candidate energetically and financially. The final results proved that both the thermal oil–ceramic rocks and the PCM are better technologies than the simple sensible thermal oil storage. For the design with a 180 m2 collecting area and 8 m3 storage tank volume, the thermal oil–ceramic rocks design leads to 13.89% system efficiency and net present value (NPV) to 129.73 k€, the PCM storage to 13.97% and 128.66 k€, respectively, while the pure thermal oil case leads to 12.48% and 105.32 k€, respectively. Moreover, it is useful to state that when the collecting area is varied from 160 m2 to 200 m2 with the tank volume at 8 m3, the efficiency enhancement with ceramic rocks compared to pure oil ranges from 8.99% up to 12.39%, while the enhancement with PCM ranges from 7.96% to 13.26%. For the same conditions, the NPV is improved with ceramic rocks from 18.35% to 25.79%, while with PCM from 14.17% to 25.29%.
Review of Biometeorology of Heatwaves and Warm Extremes in Europe
Authors: Basarin Biljana , Lukić Tin , Matzarakis Andreas
Published: November 2020

Numerous extreme heatwaves producing large impacts on human health, agriculture, water resources, energy demand, regional economies, and forest ecosystems occurred during the first twenty years of the 21st century. The present study strives to provide a systematic review of recent studies of warm biometeorological extremes in Europe. The main aim of this paper is to provide a methodical summary of the observed changes in warm extremes, duration, and variability in different parts of Europe. During the last decade, much attention has been paid to the negative impacts of heat and humidity on human health. Therefore, the human biometeorology is required to appraise the human thermal environment in a way that human thermoregulation is taken into account. In many European countries and regions, future heat exposure will indeed exceed critical levels, and a steep increase in biometeorological heatwaves and warm extremes are expected. The indices that take into account human energy balance along with weather conditions should be used to examine the impacts of extreme heatwaves on human health and should be used as a basis for the determination of acclimatization to high-heat-stress conditions. A detailed description of recent studies that have used biometeorological indices such as Physiological Equivalent Temperature (PET) and Universal Thermal Climate Index (UTCI) for the estimation of warm extremes and their influence on human health is provided. Additionally, a short overview of the existence of the heat-health warning systems (HHWS), their conceptualization, and implementation across the European continent is considered, as well as the possibilities for further investigations and implementation of effective measures and programs that could reduce the adverse health impacts.
Measuring N2O Emissions from Multiple Sources Using a Backward Lagrangian Stochastic Model
Authors: Lin Cheng-Hsien , Grant Richard H. , Johnston Cliff T.
Published: November 2020

Nitrous oxide (N2O) emissions from agricultural soil are substantially influenced by nitrogen (N) and field management practices. While routinely soil chambers have been used to measure emissions from small plots, measuring field-scale emissions with micrometeorological methods has been limited. This study implemented a backward Lagrangian stochastic (bLS) technique to simultaneously and near-continuously measure N2O emissions from four adjacent fields of approximately 1 ha each. A scanning open-path Fourier-transform infrared spectrometer (OP-FTIR), edge-of-field gas sampling and measurement, locally measured turbulence, and bLS emissions modeling were integrated to measure N2O emissions from four adjacent fields of maize production using different management in 2015. The maize N management treatments consisted of 220 kg NH3-N ha−1 applied either as one application in the fall after harvest or spring before planting or split between fall after harvest and spring before planting. The field preparation treatments evaluated were no-till (NT) and chisel plow (ChP). This study showed that the OP-FTIR plus bLS method had a minimum detection limit (MDL) of ±1.2 µg m−2 s−1 (3σ) for multi-source flux measurements. The average N2O emission of the four treatments ranged from 0.1 to 2.3 µg m−2 s−1 over the study period of 01 May to 11 June after the spring fertilizer application. The management of the full-N rate applied in the fall led to higher N2O emissions than the split-N rates applied in the fall and spring. Based on the same N application, the ChP practice tended to increase N2O emissions compared with NT. Advection of N2O from adjacent fields influenced the estimated emissions; uncertainty (1σ) in emissions was 0.5 ± 0.3 µg m−2 s−1 if the field of interest received a clean measured upwind background air, but increased to 1.1 ± 0.5 µg m−2 s−1 if all upwind sources were advecting N2O over the field of interest. Moreover, higher short-period emission rates (e.g., half-hour) were observed in this study by a factor of 1.5~7 than other micrometeorological studies measuring N2O-N loss from the N-fertilized cereal cropping system. This increment was attributed to the increase in N fertilizer input and soil temperature during the measurement. We concluded that this method could make near-continuous “simultaneous” flux comparisons between treatments, but further studies are needed to address the discrepancies in the presented values with other comparable N2O flux studies.
DKC1 Overexpression Induces a More Aggressive Cellular Behavior and Increases Intrinsic Ribosomal Activity in Immortalized Mammary Gland Cells
Authors: Guerrieri Ania Naila , Zacchini Federico , Onofrillo Carmine , Di Viggiano Sara , Penzo Marianna , Ansuini Alessio , Gandin Ilaria , Nobe Yuko , Taoka Masato , Isobe Toshiaki , Treré Davide , Montanaro Lorenzo
Published: November 2020

Dyskerin is a nucleolar protein involved in the small nucleolar RNA (snoRNA)-guided pseudouridylation of specific uridines on ribosomal RNA (rRNA), and in the stabilization of the telomerase RNA component (hTR). Loss of function mutations in DKC1 causes X-linked dyskeratosis congenita, which is characterized by a failure of proliferating tissues and increased susceptibility to cancer. However, several tumors show dyskerin overexpression. We observed that patients with primary breast cancers with high dyskerin levels are more frequently characterized by shorter survival rates and positive lymph node status than those with tumors with a lower dyskerin expression. To functionally characterize the effects of high dyskerin expression, we generated stably overexpressing DKC1 models finding that increased dyskerin levels conferred a more aggressive cellular phenotype in untransformed immortalized MCF10A cells. Contextually, DKC1 overexpression led to an upregulation of some snoRNAs, including SNORA67 and a significantly increased U1445 modification on 18S rRNA, the known target of SNORA67. Lastly, we found that dyskerin overexpression strongly enhanced the synthetic activity of ribosomes increasing translational efficiency in MCF10A. Altogether, our results indicate that dyskerin may sustain the neoplastic phenotype from an early stage in breast cancer endowing ribosomes with an augmented translation efficiency.
Ga-Substituted Cobalt-Chromium Spinels as Ceramic Pigments Produced by Sol–Gel Synthesis
Authors: Grazenaite Egle , Garskaite Edita , Stankeviciute Zivile , Raudonyte-Svirbutaviciene Eva , Zarkov Aleksej , Kareiva Aivaras
Published: November 2020

For the first time to the best of our knowledge, cobalt-chromium spinels CoCr2−xGaxO4 with different amounts of gallium (x = 0–2 with a step of 0.5) were synthesized via the aqueous sol–gel route as ceramic pigments. The phase composition, crystallite size, morphological features, and color parameters of new compositions and their corresponding ceramic glazes were investigated using XRD, CIELab, SEM, and optical microscopy. It was demonstrated that the formation of single-phase CoCr2−xGaxO4 samples was problematic. Full substitution of Cr3+ by Ga3+ ion in the spinel resulted in the formation of light blue powders, which yielded violetish blue color for the corresponding ceramic glaze.
Recent Advancements of N-Doped Graphene for Rechargeable Batteries: A Review
Authors: Ikram Rabia , Mohamed Jan Badrul , Atif Pervez Syed , Papadakis Vassilis M. , Ahmad Waqas , Bushra Rani , Kenanakis George , Rana Masud
Published: November 2020

Graphene, a 2D carbon structure, due to its unique materials characteristics for energy storage applications has grasped the considerable attention of scientists. The highlighted properties of this material with a mechanically robust and highly conductive nature have opened new opportunities for different energy storage systems such as Li-S (lithium-sulfur), Li-ion batteries, and metal-air batteries. It is necessary to understand the intrinsic properties of graphene materials to widen its large-scale applications in energy storage systems. In this review, different routes of graphene synthesis were investigated using chemical, thermal, plasma, and other methods along with their advantages and disadvantages. Apart from this, the applications of N-doped graphene in energy storage devices were discussed.
True Amplitude Angle Gathers from Reverse Time Migration by Wavefield Decomposition at Excitation Amplitude Time
Authors: Yan Zhe , Yang Yonglong , Liu Shaoyong
Published: November 2020

Reservoir parameter estimation is one of the goals of amplitude-versus-angle (AVA) inversion and angle-domain common image gathers are the basis of AVA inversion. Therefore, the accuracy of kinematic and kinetic information on angle gathers is very important for reservoir characterization. Reverse time migration is one of the most physically accurate migration method. Generating angle gathers from reverse time migration with the Poynting vector method is very efficient. However, due to inaccurate angle measurement and uneven illumination, angle gathers calculated by the Poynting vector method are often not suitable for AVA inversion. In this paper, we propose an efficient method of angle gathers with accurate angular information and amplitude from reverse time migration. We firstly decompose source and receiver wavefield to their up-going and down-going parts by using analytic wavefield. We calculate propagation directions for source down-going wavefield and receiver up-going wavefield by the Poynting vector method and form the angle gathers with these angle information and decomposed wavefield. To reduce memory storage and improve computational efficiency, we decompose wavefield at excitation amplitude time by using a local spatial Fourier transform. We also use a spatial smoothed Poynting vector to improve the stability of angle measurement. We apply an illumination compensation image condition to recover the true amplitude. Numerical examples on Marmousi model and the SEAM two-dimensional (2D) model demonstrate the advantages of our proposed method. The angle gathers based on our method are cleaner with more focus on events energy and better continuity, suffering from less low-frequency noise in the shallow parts and with a distinct cutoff at large angle where reflection terminates. At last, we demonstrate the effectiveness of proposed method on a 2D marine field data example.
Joint Study of Impingement Combustion Simulation and Diesel Visualization Experiment of Variable Injection Pressure in Constant Volume Vessel
Authors: Tang Yuanzhi , Lou Diming , Wang Chengguan , Tan Piqiang , Hu Zhiyuan , Zhang Yunhua , Fang Liang
Published: November 2020

In this paper, the visualization experiments of spray, ignition, and combustion of diesel under variable injection pressure (from 90 to 130 MPa) were studied by using a constant volume vessel and impinging combustion plate system. With the development of the down-sizing of diesel engines, the wall impinging combustion without liquid spray collision will be the research focus in the diesel engine combustion process. The flame natural luminosity in the experiment represents the soot formation of diesel combustion. Besides, the detailed information of diesel spray mixing combustion was obtained by using the CFD (Computational Fluid Dynamics) simulation of alternative fuels in CONVERGE™. The specific conclusions are as follows. The high velocity of the spray under the higher injection pressure could reduce the low-mixing area near the impinging wall by entraining more air. Under higher injection pressure in simulation, the gas diffused more extensively, and more heat was released after combustion. Therefore, a large amount of soot formed in the early stage of combustion and then oxidized in high-temperature regions, which agreed with the conclusions in the experiments. Under the influence of the superposition of image pixels of the flame, the change of soot generation with injection pressure is smaller than the actual value, so the visualization experiment can be used as the basis of combustion prediction.
A Methodology to Investigate the Deviations between Simple and Detailed Dynamic Methods for the Building Energy Performance Assessment
Authors: Ballarini Ilaria , Costantino Andrea , Fabrizio Enrico , Corrado Vincenzo
Published: November 2020

The research investigates the validity of the simple hourly method, as introduced by the EN ISO 52016-1 standard, for the assessment of the building energy demand for heating and cooling, by comparing it with a detailed dynamic model (EnergyPlus). A new methodology is provided to identify and quantify the causes of deviations between the models. It consists in the split of the contributions of the air heat balance (AHB) equation by dynamic driving force, and in the adoption of consistency options of the modeling parameters related to specific physical phenomena. A case study approach is adopted in the article to achieve the research objective. The results show that the deviations in the heating and cooling loads between the two calculation methods can be mainly ascribed to the use of different surface heat transfer coefficients, and to a different modeling of the extra thermal radiation to the sky. Providing a methodology to validate the calculation method, this work is intended to contribute to the enhancement of the use of simple dynamic models and to the improvement of the standardization activity.
Tequila Still Distillation Fractioned Residual Streams for Use in Biorefinery
Authors: Martinez-Orozco Edgardo , Gortares-Moroyoqui Pablo , Santiago-Olivares Norberto , Napoles-Armenta Juan , Ulloa-Mercado Ruth Gabriela , De la Mora-Orozco Celia , Leyva-Soto Luis Alonso , Alvarez-Valencia Luis Humberto , Meza-Escalante Edna Rosalba
Published: November 2020

Tequila vinasses is a mixture made from up to six still distillation two-stage process residual effluents. First stage fractions: residual must (60%), heads (0.9%) and tails (20.0%); second stage fractions: non-evaporated (8.0%), heads (0.1%) and tails (1.0%); the result is a more complex effluent for its treatment or biorefining. The objectives of this study were to: (a) characterize the five still distillation volatile streams in the Tequila 100% Agave processing; compounds: methanol, ethanol, acetaldehyde, ethyl acetate, sec-butanol, n-propanol, iso-butanol, n-butanol, iso-amyl, n-amyl, and ethyl lactate were detected by gas chromatography; calculated chemical oxygen demand from chemical composition had very high values (53,760–1,239,220 mg/L); measurement of pH (3.24–4.80), color (38.6 UC Pt-Co max), turbidity (46.1 max), electrical conductivity (3.30–172.20 μS/cm), and solid content (0 mg/L) was also made; (b) report an energy analysis (2.02 × 109 KWh) and CO2 production (429 × 106 kg) in the Tequila industry during 2019; (c) up to date residues (365.2 × 106 kg agave bagasse, 1146.1 × 106 kg agave leaves and 3300.0 × 106 L agave vinasse) in 2019; (d) economic analysis, current tequila vinasses treatment price is 16.00 USD/m3 but could reach a considerable fraction value if is bio-refined, a break down component analysis reach for five volatile streams $51.23–$140.00 USD/m3.
Optimal Sizing of On-Board Energy Storage Systems and Stationary Charging Infrastructures for a Catenary-Free Tram
Authors: Yang Ying , Zhang Weige , Wei Shaoyuan , Wang Zhenpo
Published: November 2020

This paper introduces an optimal sizing method for a catenary-free tram, in which both on-board energy storage systems and charging infrastructures are considered. To quantitatively analyze the trade-off between available charging time and economic operation, a daily cost function containing a whole life-time cost of energy storage and an expense of energy supplies is formulated for the optimal sizing problem. A mixed particle swarm optimization algorithm is utilized to find optimal solutions for three schemes: (1) ultracapacitors storage systems with fast-charging at each station; (2) battery storage systems with slow-charging at starting and final stations; (3) battery storage systems with fast-swapping at swapping station. A case study on an existing catenary-free tramline in China is applied to verify the effectiveness of the proposed method. Results show that a daily-cost reduction over 30% and a weight reduction over 40% can be achieved by scheme 2, and a cost saving of 34.23% and a weight reduction of 32.46% can be obtained by scheme 3.
Estimation of Energy Efficiency Class Limits for Multi-Family Residential Buildings in Poland
Authors: Kwiatkowski Jerzy , Rucińska Joanna
Published: November 2020

The need to achieve energy consumption and greenhouse gas emission decreases within the building sector requires the implementation of a supportive legal framework. To fulfil this requirement, a building certification system must be developed that is easily understandable for end users. In Poland, assessments of the energy performance of buildings are based on primary energy indicator verification. However, this parameter is difficult to understand for building owners or for tenants. Therefore, in this study, energy efficiency class limits and a heating indicator for energy needs indicator have been estimated for multi-family buildings in Poland. To achieve this purpose, a reference building was used and 180 calculation variants were developed, which considered the following variables: 3 types of climate data, 4 values of building shape ratios, 3 types of ventilation, 3 thermal transmittance standards and 3 windows area ratios to the external wall. The results showed that the greatest influences on the need for energy used for heating were the type of ventilation used and the local climate. This study shows that the methodology used is adequate for energy efficiency class limits estimations involving multi-family buildings in Poland.
Buds, Bugs and Bienniality: The Floral Biology of Eschweilera tenuifolia (O. Berg) Miers in a Black-Water Flooded Forest, Central Amazonia
Authors: Barnett Adrian A. , Boyle Sarah A. , Kinap Natalia M. , Santos-Barnett Tereza Cristina dos , Camilo Thiago Tuma , Parolin Pia , Fernandez Piedade Maria Teresa , Bezerra Bruna M.
Published: November 2020

Research Highlights: Our study establishes the biennial nature of flowering intensity as a life-time energy-conserving strategy; we show unexpectedly high flower:fruit ratios despite extensive predation of buds and flowers by insect larvae; ‘selective’ bud abortion may be a key annual energy-saving strategy. Background and Objectives: We aim to explain the strongly biennial flowering pattern of Eschweilera tenuifolia, an ecologically key tree species of Amazon blackwater-flooded forest, inundated for up to nine months annually, and with large flowers (6 cm in width). Materials and Methods: We quantified the insect infestation of central Amazonian Eschweilera tenuifolia buds and flowers; we measured nectar production from flower opening onwards, examined flower duration and monitored pollen theft. We tested the role of infestation in bud abortion, nectar production and fruit production initiation. Results: Our study shows extensive predation of buds and flowers by insect larvae, as well as selective abortion of heavily infested buds, and limited loss to pollen thieves which fed largely on infertile fodder pollen. Nectar production peaked in the morning, with no nocturnal nectar production recorded. Sucrose levels were similar to congeneric values (mean 37.4%), and near-constant during production. Flower duration (4–5 days) was longer than reported for other congenerics. Conclusions: Insect infestation of buds can play an important role in regulating flower:fruit ratios, thus setting limits on individual total seed set. Individual Eschweilera tenuifolia appear to invest highly in reproduction every second year. Extended flower duration may be a strategy to enhance pollination success, but increases overall reproductive investment. Abortion of heavily infested buds may minimize allocation of energy to malformed flowers, which have a lower chance of attracting pollinators, thus functioning as a short-term energy-saving strategy. Additionally, biennial flowering in E. tenuifolia is likely to be an energy-conserving response in a highly physiologically-challenging environment. Thus, E. tenuifolia exhibits energy-conservation strategies at two divergent temporal scales.
Monitoring and Management of the Pine Processionary Moth in the North-Western Italian Alps
Authors: Ferracini Chiara , Saitta Valerio , Pogolotti Cristina , Rollet Ivan , Vertui Flavio , Dovigo Luca
Published: November 2020

The pine processionary moth (PPM), Thaumetopoea pityocampa (Denis and Schiffermüller, 1775) (Lepidoptera, Notodontidae), is considered one of the main insect defoliators of conifers in Southern Europe and North Africa. The species is oligophagous on pines and cedars in Mediterranean countries. This 6-year investigation (2014–2019), carried out in Aosta Valley (NW Italy), on Pinus sylvestris L. aimed to: (i) Monitor the PPM population with pheromone-baited funnel traps; (ii) assess the infestation index (0–5 classes) according to the degree of defoliation; and (iii) apply control strategies, namely Bacillus thuringiensis var. kurstaki (Btk) and mating disruption (MD). In total, 9618 ha were visually monitored and assigned an infestation index. The percentage of woodland stands that were strongly defoliated by PPM (infestation index ≥ 3) increased progressively between 2015 and 2016, affecting from 8% to 19% of the total area monitored; this area decreased to 16% and 13% in 2017 and 2018, respectively, followed by an abrupt decline to 4% in 2019. Both Btk applications and mating disruption significantly reduced the infestation. Where Btk was applied, the rate of larval mortality ranged from 79.47% to 98.43%; conversely, in the control plots, the larval mortality was, on average, 1.56%. The mean number of PPM males captured in traps was significantly lower in the plots where MD was performed, ranging from 8.36 ± 2.37 to 13.47 ± 4.68. The mean number of males captured in the control plots was, on average, 119.16 ± 12.68. The total number of nests recorded per tree was significantly lower in MD plots, ranging from 0.41 ± 0.05 to 0.94 ± 0.14. In the control plots, there were, on average, 4.37 ± 0.76 nests per tree. As already documented for several defoliating forestry insect pests, both Btk and mating disruption proved effective in controlling PPM infestations, and thus, microbial insecticides should be strongly encouraged and adopted by forest managers.
Effects of Bt-Cry1Ah1 Transgenic Poplar on Target and Non-Target Pests and Their Parasitic Natural Enemy in Field and Laboratory Trials
Authors: Wang Pu , Wei Hui , Sun Weibo , Li Lingling , Zhou Peijun , Li Dawei , Qiang Zhuge
Published: November 2020

Increasing areas of artificial afforestation and poplar monoculture in China have led to serious problems with insect pests. The development of genetic engineering technology, such as transgenic modification with Bacillus thuringiensis (Bt) genes, provides novel solutions to the pest problem. We generated a Bt-Cry1Ah1 gene incorporating codon optimization and transferred it into Populus deltoides × P. euramericana cv “Nanlin895” using an Agrobacterium-mediated method. The resulting Bt-Cry1Ah1 transgenic poplars were planted in the field with permission from the State Forestry Administration in 2017. Field and laboratory studies were conducted in Jiangsu, China, to investigate the effects of these transgenic poplars expressing the Cry1Ah1 protein on target and non-target pests and their parasitic natural enemy. Target pests included Hyphantria cunea (Lepidoptera, Arctiidae), Micromelalopha troglodyta (Lepidoptera, Notodontidae), and Clostera anachoreta (Lepidoptera, Notodontidae). Plagiodera versicolora (Coleoptera, Chrysomelidae) served as the non-target pest. Laboratory trials showed that the six transgenic poplar lines exhibited resistance against the target insects. The corrected mortality rates of the target pest larvae fed leaves from the six lines were as high as 87.0%, significantly higher than that of the control. However, the corrected mortality rate of the non-target pest larvae was markedly lower and did not differ significantly from that of the control. Field experiments showed that transgenic poplar exhibited resistance against H. cunea and M. troglodyta. Field mortality rates were slightly higher than laboratory mortality rates. In addition, we investigated Chouioia cunea (Hymenoptera, Eulophidae) as a parasitoid of H. cunea pupae that had been fed transgenic poplar leaves. The emergence time, parasitism rate, and abundance of C. cunea did not differ significantly from those of the control. Therefore, Bt-Cry1Ah1 transgenic poplar can be used to effectively control damage by target insect pests without negatively affecting non-target insects and parasitoids.
Discriminant Analysis of the Damage Degree Caused by Pine Shoot Beetle to Yunnan Pine Using UAV-Based Hyperspectral Images
Authors: Liu Mengying , Zhang Zhonghe , Liu Xuelian , Yao Jun , Du Ting , Ma Yunqiang , Shi Lei
Published: November 2020

Due to the increased frequency and intensity of forest damage caused by diseases and pests, effective methods are needed to accurately monitor the damage degree. Unmanned aerial vehicle (UAV)-based hyperspectral imaging is an effective technique for forest health surveying and monitoring. In this study, a framework is proposed for identifying the severity of damage caused by Tomicus spp. (the pine shoot beetle, PSB) to Yunnan pine (Pinus yunnanensis Franch) using UAV-based hyperspectral images. Four sample plots were set up in Shilin, Yunnan Province, China. A total of 80 trees were investigated, and their hyperspectral data were recorded. The spectral data were subjected to a one-way ANOVA. Two sensitive bands and one sensitive parameter were selected using Pearson correlation analysis and stepwise discriminant analysis to establish a diagnostic model of the damage degree. A discriminant rule was established to identify the degree of damage based on the median value between different degrees of damage. The diagnostic model with R690 and R798 as variables had the highest accuracy (R2 = 0.854, RMSE = 0.427), and the test accuracy of the discriminant rule was 87.50%. The results are important for forest damage caused by the PSB.
Rootlets, a Malting By-Product with Great Potential
Authors: Neylon Emma , Arendt Elke K. , Lynch Kieran M. , Zannini Emanuele , Bazzoli Paolo , Monin Thomas , Sahin Aylin W.
Published: November 2020

Barley rootlets are the most abundant by-product from the malting industry. Due to the inherent association of the malting industry with brewing and distilling industries, it is also considered a by-product of these industries. Barley rootlets are produced during the germination step of malting. These rootlets are a valuable source of nutrition, with protein and fibre holding a large proportion of their composition. Barley rootlets are generally pelletised and used as animal fodder; however, their usage may not be limited to this. Efforts have been made to utilise barley rootlets as food ingredients, sources of enzymes, antioxidants, raw materials in fermentations, and in biochar production. Conversion of this by-product into other/new applications would reduce waste production from their industry origin and reduce some of the impending environmental concerns associated with by-product production. The current review focuses on providing information on the formation, production, and processing of barley rootlets, while also highlighting the composition, quality, and potential applications of barley rootlets.
Risk Perceptions on Social Media Use in Norway
Authors: Nyblom Philip , Wangen Gaute , Gkioulos Vasileios
Published: November 2020

Social media are getting more and more ingrained into everybody’s lives. With people’s more substantial presence on social media, threat actors exploit the platforms and the information that people share there to deploy and execute various types of attacks. This paper focuses on the Norwegian population, exploring how people perceive risks arising from the use of social media, focusing on the analysis of specific indicators such as age, sexes and differences among the users of distinct social media platforms. For data collection, a questionnaire was structured and deployed towards the users of multiple social media platforms (total n = 329). The analysis compares risk perceptions of using the social media platforms Facebook (n = 288), Twitter (n = 134), Reddit (n = 189) and Snapchat (n = 267). Furthermore, the paper analyses the differences between the sexes and between the digital natives and non-natives. Our sample also includes sufferers of ID theft (n = 50). We analyse how account compromise occurs and how suffering ID theft changes behaviour and perception. The results show significant discrepancies in the risk perception among the social media platform users across the examined indicators, but also explicit variations on how this affects the associated usage patterns. Based on the results, we propose a generic risk ranking of social media platforms, activities, sharing and a threat model for SoMe users. The results show the lack of a unified perception of risk on social media, indicating the need for targeted security awareness enhancement mechanisms focusing on this topic.
Transcriptome Dynamics during Black and White Sesame (Sesamum indicum L.) Seed Development and Identification of Candidate Genes Associated with Black Pigmentation
Authors: Wang Linhai , Dossou Senouwa Segla Koffi , Wei Xin , Zhang Yanxin , Li Donghua , Yu Jingyin , Zhang Xiurong
Published: November 2020

Seed coat color is a crucial agronomic trait in sesame (Sesamum indicum L.) since it is strongly linked to seed oil, proteins, and lignans contents, and also influences consumer preferences. In East Asia, black sesame seed is used in the treatment and the prevention of various diseases. However, in sesame, little is known about the establishment of the seed coat color, and only one gene has been reported to control black pigmentation. This study provides an overview of developing seeds transcriptome of two varieties of sesame “Zhongfengzhi No.1” (white seed) and “Zhongzhi No.33” (black seed) and shed light on genes involving in black seed formation. Until eight days post-anthesis (DPA), both the seeds of the two varieties were white. The black sesame seed turned to yellow between 9 and 11 DPA and then black between 12 and 14 DPA. The black and white sesame showed similar trend-expressed genes with the numbers increased at the early stages of seed development. The differentially expressed genes (DEGs) number increased with seed development in the two sesame varieties. We examined the DEGs and uncovered that more were up-regulated at the early stages. The DEGs between the black and white sesame were mainly enriched in 37 metabolic pathways, among which the flavonoid biosynthesis and biosynthesis of secondary metabolites were dominants. Furthermore, we identified 20 candidate genes associated with pigment biosynthesis in black sesame seed, among which 10 were flavonoid biosynthesis and regulatory genes. These genes also include isochorismate and polyphenol oxidase genes. By comparing the phenotypes and genes expressions of the black and white sesame seed at different development stages, this work revealed the important role of 8–14 DPA in black pigment biosynthesis and accumulation. Moreover, it unfolded candidate genes associated with black pigmentation in sesame. These findings provide a vast transcriptome dataset and list of genes that will be targeted for functional studies related to the molecular mechanism involved in biosynthesis and regulation of seed coat color in sesame.
MicroRNA-214 Inhibits Chicken Myoblasts Proliferation, Promotes Their Differentiation, and Targets the TRMT61A Gene
Authors: Duan Yanjun , Wu Yulin , Yin Xuemei , Li Tingting , Chen Fuxiang , Wu Pengfei , Zhang Shanshan , Wang Jinyu , Zhang Genxi
Published: November 2020

The proliferation and differentiation of myoblasts is an important process of skeletal muscle development. In this process, microRNAs (miRNAs) play an important role in the proliferation and differentiation of chicken primary myoblasts (CPMs). Our previous study found that miR-214 and the tRNA methyltransferase 61A (TRMT61A) gene were differentially expressed in different stages of proliferation and differentiation. Therefore, this study aimed to explore the effect of miR-214 on the proliferation and differentiation of CPMs and the functional relationship between miR-214 and TRMT61A. In this study, we detected the effect of miR-214 on the proliferation of CPMs by qPCR, flow cytometry, CCK-8, and EdU after the overexpression and interference of miR-214. qPCR, Western blotting, and indirect immunofluorescence were used to detect the effect of miR-214 on the differentiation of the CPMs. The expression patterns of miR-214 and TRMT61A were observed at different time points of differentiation induced by the CPMs. The results show that miR-214 inhibited the proliferation of the CPMs and promoted the differentiation of the CPMs. The Dual-Luciferase Reporter assay and the expression pattern of miR-214 and TRMT61A suggested that they had a negative regulatory target relationship. This study revealed the function and regulatory mechanism of miR-214 in the proliferation and differentiation of CPMs.