bluuebunny/crossref_metadata_2025 huggingface dataset:
Integration of Joint Power-Heat Flexibility of Oil Refinery Industries to Uncertain Energy Markets
Authors: Golmohamadi Hessam , Asadi Amin
Published: September 2020

This paper proposes a novel approach to optimize the main energy consumptions of heavy oil refining industries (ORI) in response to electricity price uncertainties. The whole industrial sub-processes of the ORI are modeled mathematically to investigate the joint power-heat flexibility potentials of the industry. To model the refinery processes, an input/output flow-based model is proposed for five main refining units. Moreover, the role of storage tanks capacity in the power system flexibility is investigated. To hedge against the electricity price uncertainty, an uncertain bound for the wholesale electricity price is addressed. To optimize the industrial processes, a dual robust mixed-integer quadratic program (R-MIQP) is adopted; therefore, the ORI’s operational strategies are determined under the worst-case realization of the electricity price uncertainty. Finally, the suggested approach is implemented in the south-west sector of the Iran Energy Market that suffers from a lack of electricity in hot days of summer. The simulation results confirm that the proposed framework ensures industrial demand flexibility to the external grids when a power shortage occurs. The approach not only provides demand flexibility to the power system, but also minimizes the operation cost of the industries.
Simulating Fracture Sealing by Granular LCM Particles in Geothermal Drilling
Authors: Lee Lu , Dahi Taleghani Arash
Published: September 2020

Lost circulation occurs when the returned fluid is less than what is pumped into the well due to loss of fluid to pores or fractures. A lost-circulation event is a common occurrence in a geothermal well. Typical geothermal reservoirs are often under-pressured and have larger fracture apertures. A severe lost-circulation event is costly and may lead to stuck pipe, well instability, and well abandonment. One typical treatment is adding lost-circulation materials (LCMs) to seal fractures. Conventional LCMs fail to properly seal fractures because their mechanical limit is exceeded at elevated temperatures. In this paper, parametric studies in numerical simulations are conducted to better understand different thermal effects on the sealing mechanisms of LCMs. The computational fluid dynamics (CFDs) and the discrete element method (DEM) are coupled to accurately capture the true physics of sealing by granular materials. Due to computational limits, the traditional Eulerian–Eulerian approach treats solid particles as a group of continuum matter. With the advance of modern computational power, particle bridging is achievable with DEM to track individual particles by modeling their interactive forces between each other. Particle–fluid interactions can be modeled by coupling CFD algorithms. Fracture sealing capability is investigated by studying the effect of four individual properties including fluid viscosity, particle size, friction coefficient, and Young’s modulus. It is found that thermally degraded properties lead to inefficient fracture sealing.
Compressed Machine Learning Models for the Uncertainty Quantification of Power Distribution Networks
Authors: Memon Zain Anwer , Trinchero Riccardo , Manfredi Paolo , Canavero Flavio , Stievano Igor S.
Published: September 2020

Today’s spread of power distribution networks, with the installation of a significant number of renewable generators that depend on environmental conditions and on users’ consumption profiles, requires sophisticated models for monitoring the power flow, regulating the electricity market, and assessing the reliability of power grids. Such models cannot avoid taking into account the variability that is inherent to the electrical system and users’ behavior. In this paper, we present a solution for the generation of a compressed surrogate model of the electrical state of a realistic power network that is subject to a large number (on the order of a few hundreds) of uncertain parameters representing the power injected by distributed renewable sources or absorbed by users with different consumption profiles. Specifically, principal component analysis is combined with two state-of-the-art surrogate modeling strategies for uncertainty quantification, namely, the least-squares support vector machine, which is a nonparametric regression belonging to the class of machine learning methods, and the widely adopted polynomial chaos expansion. Such methods allow providing compact and efficient surrogate models capable of predicting the statistical behavior of all nodal voltages within the network as functions of its stochastic parameters. The IEEE 8500-node test feeder benchmark with 450 and 900 uncertain parameters is considered as a validation example in this study. The feasibility and strength of the proposed method are verified through a systematic assessment of its performance in terms of accuracy, efficiency, and convergence, based on reference simulations obtained via classical Monte Carlo analysis.
A Practical Approach to Optimising Distribution Transformer Tap Settings
Authors: Paoli Joshua , Brinkmann Bernd , Negnevitsky Michael
Published: September 2020

This paper proposes a method of determining the optimal tap settings for no-load distribution transformers with tap-changing capabilities that is practical to apply in real distribution networks. The risk of low voltage distribution networks violating voltage constraints is impacted by the increasing uptake of distributed energy resources and embedded generation. Some of this risk can be alleviated by suitably setting no-load transformer tap settings, however, modifying these taps requires customer outages and must be infrequent. Hence, loading over the entire year must be considered to account for seasonal variations when setting these taps optimally. These settings are determined using evolution strategy optimisation based on an average loading case. Monte Carlo simulations are used to calculate the probability that the terminal voltages on the distribution transformer secondary terminals violate the network voltage limits when the optimal set of taps for the average case is applied over a whole year. This algorithm was tested on several cases of a real distribution feeder of varying complexity, and produces a sufficiently-optimal set of taps without significant computation time.
Temperature Effect of Nano-Structure Rebuilding on Removal of DWS mc-Si Marks by Ag/Cu MACE Process and Solar Cell
Authors: Pu Tian , Shen Honglie , Zheng Chaofan , Xu Yajun , Jiang Ye , Tang Quntao , Yang Wangyang , Rui Chunbao , Li Yufang
Published: September 2020

The absence of an effective texturing technique for diamond-wire sawn multi-crystalline silicon (DWS mc-Si) solar cells has hindered commercial upgrading from traditional multi-wire slurry sawn silicon (MWSS mc-Si) solar cells. In this work, we present a novel method for the removal of diamond-wire-sawn marks in a multi-crystalline silicon wafer based on metal assisted chemical etching process with Cu/Ag dual elements and nano-structure rebuilding (NSR) treatment to make a uniform inverted pyramid textured structure. The temperature effect of NSR solution was systematically analyzed. It was found that the size of the inverted pyramid structure and the reflectance became larger with the increase of the NSR treatment temperature. Furthermore, the prepared unique inverted pyramid structure not only benefited light trapping, but also effectively removed the saw-marks of the wafer at the same time. The highest efficiency of 19.77% was obtained in solar cells with an inverted pyramid structure (edge length of 600 nm) fabricated by NSR treatment at 50 °C for 360 s, while its average reflectance was 16.50% at a 400–900 nm wavelength range.
Research on the Self-Repairing Model of Outliers in Energy Data Based on Regional Convergence
Authors: Li Nan , Zhao Xunwen , Mu Hailin , Li Yimeng , Pang Jingru , Jiang Yuqing , Jin Xin , Pei Zhenwei
Published: September 2020

The need for the statistical stability of data is increasing nowadays as the data resource has become a more and more important production factor. In this study, a set of general identification and correction models are established for data outlier modification. The research object we chose is the data of per capita energy consumption. Based on the joint diagnosis method of outliers and the regional convergence theory, the abrupt outliers are identified and corrected. The study finds that there is an outlier in the data of the Ningxia Hui Autonomous Region. According to the club grouping method, 30 provinces in China are divided into two clubs and the Ningxia Hui Autonomous Region is determined to be in the first club. We calculate the convergence rate and obtain the correction results combining the half-life cycle model.
An Ecological and Economical Assessment of Integrated Amaranth (Amaranthus hybridus) and Nile Tilapia (Oreochromis niloticus) Farming in Dar es Salaam, Tanzania
Authors: Mulokozi Deogratias Pius , Berg Håkan , Lundh Torbjörn
Published: September 2020

Organic wastes can be recycled in an ecologically sound way in fishponds by applying integrated agriculture and aquaculture systems (IAA). This kind of waste recycling can help to protect the environment from pollution and improve fishpond yields. Additionally, IAA provides an opportunity for diversification of the output from two or more existing subsystems leading to higher overall farm economic returns. This study explored the potential application of amaranth wastes (AW) as a dietary ingredient for tilapia in a tilapia-amaranths integrated system (ITA). An experimental diet (AD) contained 10% (based on the control diet, CD) inclusion of AW collected from a nearby vegetable market. The experiments included triplicate treatments with; (i) fish fed on AD, where the pond water was used for irrigating the amaranth plants (IAA-fish), and (ii) fish fed on CD, where no pond water was used for irrigating the amaranth plants (non-IAA fish). 90 days after fish stocking, eighteen 4 m2 amaranth plots were prepared and treated with (i) tap water without fertilization (control amaranths), (ii) water from IAA-fish pond and organically fertilized (IAA amaranths), and (iii) tap water and inorganically fertilized (non-IAA amaranths). The use of AW improved the fish feed conversion ratio. The overall net income from ITA was 3.2, 2.3, 2.6, and 1.8 higher than from non-IAA amaranths, IAA-amaranths, non-IAA fish, and IAA fish sub-systems respectively.
Influence of Wind Buffers on the Aero-Thermal Performance of Skygardens
Authors: Mohammadi Murtaza , Tien Paige Wenbin , Kaiser Calautit John
Published: September 2020

Many high-rise buildings have semi-enclosed landscaped spaces, which act as design elements to improve the social and environmental aspects of the building. Designs such as skygardens are open to outdoor airflow and allow occupants to observe the city skyline from a height. Due to their often high location, they are subjected to strong wind speeds and extreme environmental conditions. The current study investigates the effects of three common wind buffers (railing, hedges, and trees) located at a height of 92 m on the performance of a skygarden, in terms of occupants’ wind comfort. Computational fluid dynamics (CFD) simulations were carried out using the realisable k-epsilon method, where the vegetation was modelled as a porous zone with cooling capacity. The computational modelling of the high-rise building and vegetation were validated using previous works. The quality class (QC) of the Lawson comfort criteria was used for the evaluation of the wind comfort across the skygarden. The results indicate that, although the three wind buffers offer varying levels of wind reduction in the skygarden, the overall wind conditions generated are suitable for occupancy. Furthermore, vegetation is also able to offer slight temperature reductions in its wake. The right combination and dimension of these elements can greatly assist in generating aero-thermal comfort across skygardens.
Bioprospecting Cultivated Tropical Green Algae, Caulerpa racemosa (Forsskal) J. Agardh: A Perspective on Nutritional Properties, Antioxidative Capacity and Anti-Diabetic Potential
Authors: Qudus B Aroyehun Abdul , Abdul Razak Shariza , Palaniveloo Kishneth , Nagappan Thilahgavani , Suraiza Nabila Rahmah Nur , Wee Jin Gan , Chellappan Dinesh Kumar , Chellian Jestin , Kunnath Anil Philip
Published: September 2020

Caulerpa racemosa (Forsskal) J. Agardh is a green seaweed used as food and folk medicine since ancient times in the Indo-Pacific region, particularly in southeast Asia. In this study, the proximate nutrient composition, phytochemical, anti-oxidant and anti-diabetic properties of sea grape C. racemosa collected from culture fishponds in Johor, Malaysia were analysed. The contents (dry weight basis) of carbohydrate, crude protein, crude lipids, ash and caloric value obtained were 33.42 ± 1.34%, 20.27 ± 0.14%, 4.20 ± 0.32%, 28.25 ± 0.27% and 2544.67 ± 7.04 cal g−1, respectively. The amino acid score (AAs) and biological protein value (213.43 mg g−1) indicated that C. racemosa presented a better protein quality. The most abundant fatty acids were C16:0 (palmitic acid: 63.27%), followed by C18:1 (oleic acid: 5.80%), and C18:2 ῳ6 (linoleic acid: 5.33%). The analysis of the ash content indicated that essential minerals and trace elements, such as Ca, Fe, and Mn, were present in the seaweed. The total phenolic content (TPC) and total flavonoid content (TFC) observed in the ethyl acetate extract were 17.88 ± 0.78 mg GAE g−1 and 59.43 ± 2.45 mg QE g−1, respectively. The ethyl acetate extract of C. racemosa demonstrated notable anti-diabetic activity in diabetic induced rats. The low (100 mg kg−1) and high (200 mg kg−1) doses of cultivated C. racemosa extract exhibited a significant decrease (p < 0.05) in blood glucose levels while preventing weight loss, reducing plasma AST, ALT levels as a sign of hepatoprotective effect and recording albumin levels similar to positive control in diabetic induced rats. The results support the usefulness of cultivated C. racemosa as a potential functional food.
Fabrication of Spray-Dried Microcapsules Containing Noni Juice Using Blends of Maltodextrin and Gum Acacia: Physicochemical Properties of Powders and Bioaccessibility of Bioactives during In Vitro Digestion
Authors: Zhang Chuang , Khoo Siew Lin Ada , Swedlund Peter , Ogawa Yukiharu , Shan Yang , Quek Siew Young
Published: September 2020

Microencapsulation of fermented noni juice (FNJ) into powder format could protect bioactive compounds, reduce the unpleasant odour and improve the acceptability for consumers. Blends of maltodextrin (MD) and gum acacia (GA) were used to achieve spray-drying microencapsulation of noni juice at different blending ratios. The physicochemical properties including microstructure, moisture content, water activity, particle size, bulk/tapped density, dissolution rate, ATR-FTIR and the bioaccessibility of bioactive compounds in powders during in vitro digestion were examined. Results showed that blends produced with more GA produced microcapsules with lower moisture content, water activity and bulk/tapped density, but slower powder dissolution. The ATR-FTIR results suggested that there were no significant chemical interactions between the core material and carrier or between the MD and GA in the blend powders. The spray-dried noni juice powder produced using the blends with higher ratio of GA to MD showed a better protection on the bioactive compounds, resulting in a higher bioaccessibility of powders during in vitro digestion. This study provides insights into microencapsulation of noni juice using blends of MD and GA and examines the physicochemical properties and bioaccessibilities of spray-dried powders as affected by the selected carriers.
Standardization of a Radiofrequency Ablation Tool in an Ex-Vivo Porcine Liver Model
Authors: Rossi Gemma , Petrone Maria Chiara , Capurso Gabriele , Albarello Luca , Testoni Sabrina Gloria Giulia , Archibugi Livia , Schiavo Lena Marco , Doglioni Claudio , Arcidiacono Paolo Giorgio
Published: September 2020

(1) Background: Preclinical and clinical data about a novel radiofrequency ablation (RFA) system (STARmed Co, Ltd.; Koyang, Korea) designed to be used under endoscopic ultrasound (EUS) control for pancreatic lesion ablation, are limited, obtained with non-standardized procedures and heterogeneous results. The aim of this study is to standardize the RFA procedure of this system in order to define the optimal ablation power and time. (2) Methods: RFA was performed on an ex-vivo porcine liver at different powers (40, 30, 20, 10 Watts (W)) and times (1, 3, 5, 7, 15 min) with a 1-centimeter monopolar electrode (perfused by chilled solution) positioned on the distal tip of a 19-Gauge needle. A blinded expert pathologist histologically analyzed each ablation area. (3) Results: The size of the total macroscopic ablated area was negatively correlated with ablation power (R −0.74): the largest was obtained at 10 W (p = 4.7 × 10−4) for longer times (R 0.92; p = 8.9 × 10−8). Central histologic coagulative necrosis did not differ among ablation settings (mean size 3.25 mm). External “parenchymal hypochromia” or “diaphanization” resulted the widest at 10 W, for longer times (R 0.8, p = 3.6 × 10−4). (4) Conclusions: The RFA system can produce small sizes of coagulative necrosis, regardless of the setting. Larger areas of diaphanization surrounding the necrosis can be produced at lower powers for longer times.
Sedentary Behavior in Older Patients before and after Total Hip Arthroplasty: A Prospective Cohort Study
Authors: Moellenbeck Burkhard , Horst Frank , Gosheger Georg , Theil Christoph , Seeber Leonie , Kalisch Tobias
Published: September 2020

Objective: To compare the habitual sedentary behavior (SB) and physical activity (PA) of older hip osteoarthritis patients before and after elective arthroplasty. Methods: SB, PA and joint-specific disability of 16 patients (68.9 ± 6.8 years) were assessed by accelerometry and questionnaires before and 9 months after arthroplasty. Results: All patients reported substantial postoperative improvements of their joint-related complaints (p ≤ 0.001). Accelerometry showed changes in neither daily SB (10–60 min sedentary bouts, p ≥ 0.569) nor in PA (steps, time in mild-to-vigorous activity and energy expenditure, p ≥ 0.255). Correlation analyses revealed that patients with severe preoperative disability showed a decrease in sedentary time, which was the opposite in patients with mild preoperative disability. Conclusion: SB and PA do not necessarily change after arthroplasty in older orthopedic patients. Even longer bouts of uninterrupted sitting, which are detrimental to health, do not decrease. Preoperative patient education is recommended to foster behavioral changes following elective arthroplasty.
Visit Probability in Space–Time Prisms Based on Binomial Random Walk
Authors: Elias Deepak , Kuijpers Bart
Published: September 2020

Space–time prisms are used to model the uncertainty of space–time locations of moving objects between (for instance, GPS-measured) sample points. However, not all space–time points in a prism are equally likely and we propose a simple, formal model for the so-called “visit probability” of space–time points within prisms. The proposed mathematical framework is based on a binomial random walk within one- and two-dimensional space–time prisms. Without making any assumptions on the random walks (we do not impose any distribution nor introduce any bias towards the second anchor point), we arrive at the conclusion that binomial random walk-based visit probability in space–time prisms corresponds to a hypergeometric distribution.
Sex-Specific Effects of Microglia-Like Cell Engraftment during Experimental Autoimmune Encephalomyelitis
Authors: Han Jinming , Zhu Keying , Zhou Kai , Hakim Ramil , Sankavaram Sreenivasa Raghavan , Blomgren Klas , Lund Harald , Zhang Xing-Mei , Harris Robert A.
Published: September 2020

Multiple sclerosis (MS) is a chronic neuroinflammatory disorder of the central nervous system (CNS) that usually presents in young adults and predominantly in females. Microglia, a major resident immune cell in the CNS, are critical players in both CNS homeostasis and disease. We have previously demonstrated that microglia can be efficiently depleted by the administration of tamoxifen in Cx3cr1CreER/+Rosa26DTA/+ mice, with ensuing repopulation deriving from both the proliferation of residual CNS resident microglia and the engraftment of peripheral monocyte-derived microglia-like cells. In this study, tamoxifen was administered to Cx3cr1CreER/+Rosa26DTA/+ and Cx3cr1CreER/+ female and male mice. Experimental autoimmune encephalomyelitis (EAE), a widely used animal model of MS, was induced by active immunization with myelin oligodendrocyte glycoprotein (MOG) one month after tamoxifen injections in Cx3cr1CreER/+Rosa26DTA/+ mice and Cx3cr1CreER/+ mice, a time point when the CNS niche was colonized by microglia derived from both CNS microglia and peripherally-derived macrophages. We demonstrate that engraftment of microglia-like cells following microglial depletion exacerbated EAE in Cx3cr1CreER/+Rosa26DTA/+ female mice as assessed by clinical symptoms and the expression of CNS inflammatory factors, but these findings were not evident in male mice. Higher major histocompatibility complex class II expression and cytokine production in the female CNS contributed to the sex-dependent EAE severity in mice following engraftment of microglia-like cells. An underestimated yet marked sex-dependent microglial activation pattern may exist in the injured CNS during EAE.
Antimicrobial Effects of Thymosin Beta-4 and Ciprofloxacin Adjunctive Therapy in Pseudomonas aeruginosa Induced Keratitis
Authors: Carion Thomas W. , Ebrahim Abdul Shukkur , Alluri Spandana , Ebrahim Thanzeela , Parker Tressa , Burns Julia , Sosne Gabriel , Berger Elizabeth A.
Published: September 2020

Prior work has indicated that thymosin beta 4 (Tβ4) administered with ciprofloxacin markedly improves disease outcome for Pseudomonas aeruginosa (PA)-induced keratitis. As a result, the goal of the current study was to elucidate mechanisms by which Tβ4 mitigates the corneal response; specifically, regarding its bactericidal influence and potential synergy with ciprofloxacin. An in vitro approach was carried out using minimum inhibitory concentration (MIC) assays to assess bactericidal activity against PA. In addition, antimicrobial peptide (AMP) production was evaluated at the mRNA levels using human corneal epithelial cells in response to lipopolysaccharide (LPS) challenge. The results of the MIC assays did not show direct bactericidal activity with Tβ4 alone, although ciprofloxacin exhibited significant killing at concentrations far lower than clinically dosed. Tβ4, however, displayed an indirect effect on bacterial killing, as shown by an upregulation of AMPs and related molecules. The cumulative data from this study indicate an indirect bactericidal role of Tβ4, as well as a synergistic relationship with ciprofloxacin. Furthermore, ciprofloxacin alone was found to influence cellular functions that otherwise have yet to be reported. These results highlight a mechanism of intracellular communication for Tβ4 and further strengthen its development as an adjunct therapy with antibiotics for corneal infections.
Involvement of Capsaicin-Sensitive Lung Vagal Neurons and TRPA1 Receptors in Airway Hypersensitivity Induced by 1,3-β-D-Glucan in Anesthetized Rats
Authors: Lin You Shuei , Huang I-Hsuan , Lan Sheng-Hsuan , Chen Chia-Ling , Chen Yueh-Yin , Chan Nai-Ju , Hsu Chun-Chun
Published: September 2020

Airway exposure to 1,3-β-D-glucan (β-glucan), an essential component of the cell wall of several pathogenic fungi, causes various adverse responses, such as pulmonary inflammation and airway hypersensitivity. The former response has been intensively investigated; however, the mechanism underlying β-glucan-induced airway hypersensitivity is unknown. Capsaicin-sensitive lung vagal (CSLV) afferents are very chemosensitive and stimulated by various insults to the lungs. Activation of CSLV afferents triggers several airway reflexes, such as cough. Furthermore, the sensitization of these afferents is known to contribute to the airway hypersensitivity during pulmonary inflammation. This study was carried out to determine whether β-glucan induces airway hypersensitivity and the role of the CSLV neurons in this hypersensitivity. Our results showed that the intratracheal instillation of β-glucan caused not only a distinctly irregular pattern in baseline breathing, but also induced a marked enhancement in the pulmonary chemoreflex responses to capsaicin in anesthetized, spontaneously breathing rats. The potentiating effect of β-glucan was found 45 min later and persisted at 90 min. However, β-glucan no longer caused the irregular baseline breathing and the potentiating of pulmonary chemoreflex responses after treatment with perineural capsaicin treatment that blocked the conduction of CSLV fibers. Besides, the potentiating effect of β-glucan on pulmonary chemoreflex responses was significantly attenuated by N-acetyl-L-cysteine (a ROS scavenger), HC-030031 (a TRPA1 antagonist), and Laminarin (a Dectin-1 antagonist). A combination of Laminarin and HC-030031 further reduced the β-glucan-induced effect. Indeed, our fiber activity results showed that the baseline fiber activity and the sensitivity of CSLV afferents were markedly elevated by β-glucan instillation, with a similar timeframe in anesthetized, artificially ventilated rats. Moreover, this effect was reduced by treatment with HC-030031. In isolated rat CSLV neurons, the β-glucan perfusion caused a similar pattern of potentiating effects on capsaicin-induced Ca2+ transients, and β-glucan-induced sensitization was abolished by Laminarin pretreatment. Furthermore, the immunofluorescence results showed that there was a co-localization of TRPV1 and Dectin-1 expression in the DiI-labeled lung vagal neurons. These results suggest that CSLV afferents play a vital role in the airway hypersensitivity elicited by airway exposure to β-glucan. The TRPA1 and Dectin-1 receptors appear to be primarily responsible for generating β-glucan-induced airway hypersensitivity.
Prognostic Value of the Overexpression of Fatty Acid Metabolism-Related Enzymes in Squamous Cell Carcinoma of the Head and Neck
Authors: Su Ying-Wen , Wu Pao-Shu , Lin Sheng-Hsiang , Huang Wen-Yu , Kuo Yu-Shao , Lin Hung-Pin
Published: September 2020

Reprogramming of cellular energy metabolism, such as lipid metabolism, is a hallmark of squamous cell carcinoma of the head and neck (SCCHN). However, whether protein expression related to fatty acid oxidation (FAO) affects survival in SCCHN remains unclear. We aimed to investigate FAO-related enzyme expression and determine its correlation with clinicopathological variables in SCCHN patients. Immunohistochemical analysis (IHC) of FAO-related protein expression, including carnitine palmitoyltransferase 1 (CPT1), the acyl-CoA dehydrogenase family, and fatty acid synthase (FAS), was performed using tissue microarrays from 102 resected SCCHN tumors. Expressions were categorized according to IHC scores, and the statistical association with clinicopathological factors was determined. Moderate-to-high expression of long-chain acyl-CoA dehydrogenase (LCAD) had a protective role against cancer-related death (adjusted hazard ratio (HR), 0.2; 95% confidence interval (CI), 0.05–0.87) after covariate adjustment. Age and clinical stage remained independent predictors of survival (adjusted HR, 1.75; 95% CI, 1.22–2.49 for age; adjusted HR, 14.33; 95% CI, 1.89–108.60 for stage III/IV disease). Overexpression of medium-chain acyl-CoA dehydrogenase and FAS correlated with advanced tumor stage (T3/T4); however, none of these factors were independent predictors of survival. Several FAO-related enzymes were upregulated and LCAD overexpression had a protective effect on overall survival in advanced SCCHN patients. FAO-related-enzyme expression might have a prognostic impact on survival outcomes in SCCHN.
Advantages and Pitfalls of Capillary Electrophoresis of Pharmaceutical Compounds and Their Enantiomers in Complex Samples: Comparison of Hydrodynamically Opened and Closed Systems
Authors: Masár Marián , Hradski Jasna , Schmid Martin G. , Szucs Roman
Published: September 2020

Several research disciplines require fast, reliable and highly automated determination of pharmaceutically active compounds and their enantiomers in complex biological matrices. To address some of the challenges of Capillary Electrophoresis (CE), such as low concentration sensitivity and performance degradation linked to the adsorption and interference of matrix components, CE in a hydrodynamically closed system was evaluated using the model compounds Pindolol and Propranolol. Some established validation parameters such as repeatability of injection efficiency, resolution and sensitivity were used to assess its performance, and it was found to be broadly identical to that of hydrodynamically opened systems. While some reduction in separation efficiency was observed, this was mainly due to dispersion caused by injection and it had no impact on the ability to resolve enantiomers of model compounds even when spiked into complex biological matrix such as blood serum. An approximately 18- to 23-fold increase in concentration sensitivity due to the employment of wide bore capillaries was observed. This brings the sensitivity of CE to a level similar to that of liquid chromatography techniques. In addition to this benefit and unlike in hydrodynamically opened systems, suppression of electroosmotic flow, which is essential for hydrodynamically closed systems practically eliminates the matrix effects that are linked to protein adsorption.
Preventive Triple Gene Therapy Reduces the Negative Consequences of Ischemia-Induced Brain Injury after Modelling Stroke in a Rat
Authors: Markosyan Vage , Safiullov Zufar , Izmailov Andrei , Fadeev Filip , Sokolov Mikhail , Kuznetsov Maksim , Trofimov Dmitry , Kim Evgeny , Kundakchyan Grayr , Gibadullin Airat , Salafutdinov Ilnur , Nurullin Leniz , Bashirov Farid , Islamov Rustem
Published: September 2020

Currently, the main fundamental and clinical interest for stroke therapy is focused on developing a neuroprotective treatment of a penumbra region within the therapeutic window. The development of treatments for ischemic stroke in at-risk patients is of particular interest. Preventive gene therapy may significantly reduce the negative consequences of ischemia-induced brain injury. In the present study, we suggest the approach of preventive gene therapy for stroke. Adenoviral vectors carrying genes encoding vascular endothelial growth factor (VEGF), glial cell-derived neurotrophic factor (GDNF) and neural cell adhesion molecule (NCAM) or gene engineered umbilical cord blood mononuclear cells (UCB-MC) overexpressing recombinant VEGF, GDNF, and NCAM were intrathecally injected before distal occlusion of the middle cerebral artery in rats. Post-ischemic brain recovery was investigated 21 days after stroke modelling. Morphometric and immunofluorescent analysis revealed a reduction of infarction volume accompanied with a lower number of apoptotic cells and decreased expression of Hsp70 in the peri-infarct region in gene-treated animals. The lower immunopositive areas for astrocytes and microglial cells markers, higher number of oligodendrocytes and increased expression of synaptic proteins suggest the inhibition of astrogliosis, supporting the corresponding myelination and functional recovery of neurons in animals receiving preventive gene therapy. In this study, for the first time, we provide evidence of the beneficial effects of preventive triple gene therapy by an adenoviral- or UCB-MC-mediated intrathecal simultaneous delivery combination of vegf165, gdnf, and ncam1 on the preservation and recovery of the brain in rats with subsequent modelling of stroke.
Extracellular Vesicles as Innovative Tool for Diagnosis, Regeneration and Protection against Neurological Damage
Authors: Andjus Pavle , Kosanović Maja , Milićević Katarina , Gautam Mukesh , Vainio Seppo J. , Jagečić Denis , Kozlova Elena N. , Pivoriūnas Augustas , Chachques Juan-Carlos , Sakaj Mirena , Brunello Giulia , Mitrecic Dinko , Zavan Barbara
Published: September 2020

Extracellular vesicles (EVs) have recently attracted a great deal of interest as they may represent a new biosignaling paradigm. According to the mode of biogenesis, size and composition, two broad categories of EVs have been described, exosomes and microvesicles. EVs have been shown to carry cargoes of signaling proteins, RNA species, DNA and lipids. Once released, their content is selectively taken up by near or distant target cells, influencing their behavior. Exosomes are involved in cell–cell communication in a wide range of embryonic developmental processes and in fetal–maternal communication. In the present review, an outline of the role of EVs in neural development, regeneration and diseases is presented. EVs can act as regulators of normal homeostasis, but they can also promote either neuroinflammation/degeneration or tissue repair in pathological conditions, depending on their content. Since EV molecular cargo constitutes a representation of the origin cell status, EVs can be exploited in the diagnosis of several diseases. Due to their capability to cross the blood–brain barrier (BBB), EVs not only have been suggested for the diagnosis of central nervous system disorders by means of minimally invasive procedures, i.e., “liquid biopsies”, but they are also considered attractive tools for targeted drug delivery across the BBB. From the therapeutic perspective, mesenchymal stem cells (MSCs) represent one of the most promising sources of EVs. In particular, the neuroprotective properties of MSCs derived from the dental pulp are here discussed.
Optimization for Liquid-Liquid Extraction of Cd(II) over Cu(II) Ions from Aqueous Solutions Using Ionic Liquid Aliquat 336 with Tributyl Phosphate
Authors: Lee Lai Yee , Morad Norhashimah , Ismail Norli , Talebi Amir , Rafatullah Mohd
Published: September 2020

This study investigates the separation of two heavy metals, Cd(II) and Cu(II), from the mixed synthetic feed using a liquid-liquid extraction. The current study uses tri-octyl methylammonium chloride (Aliquat 336) as the extractant (with tributyl phosphate (TBP) as a phase modifier), diluted in toluene, in order to investigate the selective extraction of Cd(II) over Cu(II) ions. We investigate the use of ethylenediaminetetraacetic acid (EDTA) as a masking agent for Cu(II), when added in aqueous feed, for the selective extraction of Cd(II). Five factors that influence the selective extraction of Cd(II) over Cu(II) (the equilibrium pH (pHeq), Aliquat 336 concentration (Aliquat 336), TBP concentration (TBP), EDTA concentration (EDTA), and organic to aqueous ratio (O:A)) were analyzed. Results from a 25–1 fractional factorial design show that Aliquat 336 significantly influenced Cd(II) extraction, whereas EDTA was statistically significant for the antagonistic effect on the E% of Cu(II) in the same system. Moreover, results from optimization experiment showed that the optimum conditions are Aliquat 336 concentration of 99.64 mM and EDTA concentration of 48.86 mM—where 95.89% of Cd(II) was extracted with the least extracted Cu(II) of 0.59%. A second-order model was fitted for optimization of Cd(II) extraction with a R2 value of 0.998, and ANOVA results revealed that the model adequately fitted the data at a 5% significance level. Interaction between Aliquat 336 and Cd(II) has been proven via FTIR qualitative analysis, whereas the addition of TBP does not affect the extraction mechanism.
Obinutuzumab-Induced B Cell Depletion Reduces Spinal Cord Pathology in a CD20 Double Transgenic Mouse Model of Multiple Sclerosis
Authors: Breakell Thomas , Tacke Sabine , Schropp Verena , Zetterberg Henrik , Blennow Kaj , Urich Eduard , Kuerten Stefanie
Published: September 2020

B cell-depleting therapies have recently proven to be clinically highly successful in the treatment of multiple sclerosis (MS). This study aimed to determine the effects of the novel type II anti-human CD20 (huCD20) monoclonal antibody (mAb) obinutuzumab (OBZ) on spinal cord degeneration in a B cell-dependent mouse model of MS. Double transgenic huCD20xHIGR3 (CD20dbtg) mice, which express human CD20, were immunised with the myelin fusion protein MP4 to induce experimental autoimmune encephalomyelitis (EAE). Both light and electron microscopy were used to assess myelination and axonal pathology in mice treated with OBZ during chronic EAE. Furthermore, the effects of the already established murine anti-CD20 antibody 18B12 were assessed in C57BL/6 wild-type (wt) mice. In both models (18B12/wt and OBZ/CD20dbtg) anti-CD20 treatment significantly diminished the extent of spinal cord pathology. While 18B12 treatment mainly reduced the extent of axonal pathology, a significant decrease in demyelination and increase in remyelination were additionally observed in OBZ-treated mice. Hence, the data suggest that OBZ could have neuroprotective effects on the CNS, setting the drug apart from the currently available type I anti-CD20 antibodies.
Psoriasis and Its Impact on In-Hospital Outcome in Patients Hospitalized with Acute Kidney Injury
Authors: Wild Johannes , Hobohm Lukas , Münzel Thomas , Wenzel Philip , Steinbrink Kerstin , Karbach Susanne , Keller Karsten
Published: September 2020

Background: Psoriasis is a chronic inflammatory disease which affects the body far beyond the skin. Whereas there is solid evidence that chronic skin inflammation in psoriasis drives cardiovascular disease, the impact on renal impairment and acute kidney injury (AKI) is still unclear. We aimed to analyze the impact of psoriasis on the in-hospital outcome of patients hospitalized with AKI. Methods: In this retrospective database study, we investigated data on characteristics, comorbidities, and in-hospital outcomes for all hospitalized patients with AKI stratified for concomitant psoriasis, which were collected by the Federal Office of Statistics in Germany between 2005 and 2016. Results: Among the 3,162,449 patients treated for AKI in German hospitals between 2005 and 2016, 11,985 patients (0.4%) additionally suffered from psoriasis. While the annual number of AKI patients with psoriasis increased significantly from 485 cases (4.0%) in 2005 to 1902 (15.9%) in 2016 (p < 0.001), the in-hospital mortality decreased substantially (from 24.9% in 2005 to 17.4% in 2016; p < 0.001). AKI patients with concomitant psoriasis were younger (70 (IQR; 60–78) vs. 76 (67–83) years; p < 0.001) and were more often treated with dialysis (16.3% vs. 13.6%, p < 0.001). Presence of psoriasis in AKI patients was associated with reduced prevalence of myocardial infarction (OR 0.62; p < 0.001), stroke (OR 0.85; p = 0.013), and in-hospital mortality (OR 0.75; p < 0.001). Conclusions: AKI patients with psoriasis were hospitalized in median 6 years earlier than those without. Despite younger age, we detected higher use of kidney replacement therapy in patients with psoriasis, indicating a more severe course of AKI. Our findings might improve management of these patients and contribute evidence for extracutaneous, systemic manifestations of psoriasis.
Patients with Initial Negative RT-PCR and Typical Imaging of COVID-19: Clinical Implications
Authors: Baicry Florent , Le Borgne Pierrick , Fabacher Thibaut , Behr Martin , Lemaitre Elena Laura , Gayol Paul-Albert , Harscoat Sébastien , Issur Nirvan , Garnier-Kepka Sabrina , Ohana Mickael , Bilbault Pascal , Oberlin Mathieu
Published: September 2020

The sensitivity of reverse transcriptase polymerase chain reaction (RT-PCR) has been questioned due to negative results in some patients who were strongly suspected of having coronavirus disease 2019 (COVID-19). The aim of our study was to analyze the prognosis of infected patients with initial negative RT-PCR in the emergency department (ED) during the COVID-19 outbreak. This study included two cohorts of adult inpatients admitted into the ED. All patients who were suspected to be infected with SARS-CoV-2 and who underwent a typical chest CT imaging were included. Thus, we studied two distinct cohorts: patients with positive RT-PCR (PCR+) and those with negative initial RT-PCR (PCR–). The data were analyzed using Bayesian methods. We included 66 patients in the PCR– group and 198 in the PCR+ group. The baseline characteristics did not differ except in terms of a proportion of lower chronic respiratory disease in the PCR– group. We noted a less severe clinical presentation in the PCR– group (lower respiratory rate, lower oxygen need and mechanical ventilation requirement). Hospital mortality (9.1% vs. 9.6%) did not differ between the two groups. Despite an initially less serious clinical presentation, the mortality of patients infected by SARS-CoV-2 with a negative RT-PCR did not differ from those with positive RT-PCR.
The Mechanical and Material Designs of Composite Ju|’hoansi Arrowheads
Authors: Archer Courtney , Campbell Iain , Cheyne Fraser , Meng Amos Lim Jun , Senanszky Csongor , Mamalis Dimitrios , Robert Colin , Alam Parvez
Published: September 2020

In this paper, we elucidate the composite engineering design skills of the Kalahari Ju|’hoansi (San) people, developed over at least tens of thousands of years. In particular, we show that the mechanical and physical properties of materials used by the Ju|’hoansi in the design of arrowheads are intimately linked to their unique geometrical and composite designs. The Ju|’hoansi arrowheads have evolved to become complex engineered units with distinct function-specific purpose. We demonstrate herein that the geometrical designs of the arrowheads are optimised with respect to the material used. We furthermore verify the veracity of the Ju|’hoansi claim that their composite link-shafts are designed to break off leaving the arrowhead in the animal and the remaining parts of the arrow intact, reusable and easily retrievable.
Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis
Authors: Ha Seungshin , Tripathi Prem P. , Daza Ray A. , Hevner Robert F. , Beier David R.
Published: September 2020

We have previously described hypomorphic reelin (Reln) mutant mice, RelnCTRdel, in which the morphology of the dentate gyrus is distinct from that seen in reeler mice. In the RelnCTRdel mutant, the infrapyramidal blade of the dentate gyrus fails to extend, while the suprapyramidal blade forms with a relatively compact granule neuron layer. Underlying this defect, we now report several developmental anomalies in the RelnCTRdel dentate gyrus. Most strikingly, the distribution of Cajal-Retzius cells was aberrant; Cajal-Retzius neurons were increased in the suprapyramidal blade, but were greatly reduced along the subpial surface of the prospective infrapyramidal blade. We also observed multiple abnormalities of the fimbriodentate junction. Firstly, progenitor cells were distributed abnormally; the “neurogenic cluster” at the fimbriodentate junction was absent, lacking the normal accumulation of Tbr2-positive intermediate progenitors. However, the number of dividing cells in the dentate gyrus was not generally decreased. Secondly, a defect of secondary glial scaffold formation, limited to the infrapyramidal blade, was observed. The densely radiating glial fibers characteristic of the normal fimbriodentate junction were absent in mutants. These fibers might be required for migration of progenitors, which may account for the failure of neurogenic cluster formation. These findings suggest the importance of the secondary scaffold and neurogenic cluster of the fimbriodentate junction in morphogenesis of the mammalian dentate gyrus. Our study provides direct genetic evidence showing that normal RELN function is required for Cajal-Retzius cell positioning in the dentate gyrus, and for formation of the fimbriodentate junction to promote infrapyramidal blade extension.
An Agent-Based System for Automated Configuration and Coordination of Robotic Operations in Real Time—A Case Study on a Car Floor Welding Process
Authors: Makris Sotiris , Alexopoulos Kosmas , Michalos George , Sardelis Andreas
Published: September 2020

This paper investigates the feasibility of using an agent-based framework to configure, control and coordinate dynamic, real-time robotic operations with the use of ontology manufacturing principles. Production automation agents use ontology models that represent the knowledge in a manufacturing environment for control and configuration purposes. The ontological representation of the production environment is discussed. Using this framework, the manufacturing resources are capable of autonomously embedding themselves into the existing manufacturing enterprise with minimal human intervention, while, at the same time, the coordination of manufacturing operations is achieved without extensive human involvement. The specific framework was implemented, tested and validated in a feasibility study upon a laboratory robotic assembly cell with typical industrial components, using real data derived from a car-floor welding process.
Electronic Constant Twist Angle Control System Suitable for Torsional Vibration Tuning of Propulsion Systems
Authors: Homišin Jaroslav , Kaššay Peter , Urbanský Matej , Puškár Michal , Grega Robert , Krajňák Jozef
Published: September 2020

Currently, great emphasis on reducing energy consumption and harmful emissions of internal combustion engines is placed. Current control technology allows us to customize the operating mode according to the currently required output parameters, while the tuning of mechanical systems in terms of torsional vibration is often ignored. This article deals with a semi-active torsional vibroisolation system using pneumatic flexible shaft coupling with constant twist angle control. This system is suitable, as it is specially designed, for the tuning of mechanical systems where the load torque has fan characteristics (fans, ship propellers, pumps). The main goal of this research is to verify the ability of an electronic control system developed by us to maintain the pre-set constant twist angle of the used pneumatic flexible shaft coupling during operation. The constant twist angle control function was tested on a laboratory torsional oscillating mechanical system. Presented results show that the proposed electronic control system meets the requirements for its function, namely that it can achieve, sufficiently accurately and quickly, the desired constant twist angle of the pneumatic flexible shaft coupling. It is possible to assume that the presented system will increase the technical level of the equipment where it will be applied.