bluuebunny/crossref_metadata_2025 huggingface dataset:
Transition-Metal-Doping of CaO as Catalyst for the OCM Reaction, a Reality Check
Authors: Thum Lukas , Riedel Wiebke , Milojevic Natasa , Guan Chengyue , Trunschke Annette , Dinse Klaus-Peter , Risse Thomas , Schomäcker Reinhard , Schlögl Robert
Published: February 2022

In this study, first-row transition metal-doped calcium oxide materials (Mn, Ni, Cr, Co., and Zn) were synthesized, characterized, and tested for the OCM reaction. Doped carbonate precursors were prepared by a co-precipitation method. The synthesis parameters were optimized to yield materials with a pure calcite phase, which was verified by XRD. EPR measurements on the doped CaO materials indicate a successful substitution of Ca2+ with transition metal ions in the CaO lattice. The materials were tested for their performance in the OCM reaction, where a beneficial effect towards selectivity and activity effect could be observed for Mn, Ni, and Zn-doped samples, where the selectivity of Co- and Cr-doped CaO was strongly reduced. The optimum doping concentration could be identified in the range of 0.04-0.10 atom%, showing the strongest decrease in the apparent activation energy, as well as the maximum increase in selectivity.
A Prediction Model for Acute Kidney Injury After Pericardiectomy: An Observational Study
Authors: Wang Jin , Yu Chunhua , Zhang Yuelun , Huang Yuguang
Published: February 2022

Objectives

Acute kidney injury is a common complication after pericardiectomy for constrictive pericarditis, which predisposes patients to worse outcomes and high medical costs. We aimed to investigate potential risk factors and consequences and establish a prediction model.

Methods

We selected patients with constrictive pericarditis receiving isolated pericardiectomy from January 2013 to January 2021. Patients receiving concomittant surgery or repeat percardiectomy, as well as end-stage of renal disease were excluded. Acute kidney injury was diagnosed and classified according to the KDIGO criteria. Clinical features were compared between patients with and without postoperative acute kidney injury. A prediction model was established based on multivariable regression analysis.

Results

Among two hundred and eleven patients, ninety-five (45.0%) developed postoperative acute kidney injury, with fourty-three (45.3%), twenty-eight (29.5%), and twenty-four (25.3%) in mild, moderate and severe stages, respectively. Twenty-nine (13.7%) patients received hemofiltration. Nine (4.3%) patients died perioperatively and were all in the acute kidney injury (9.5%) group. Eleven (5.2%) patients were considered to have chronic renal dysfunction states at the 6-month postoperative follow-up, and eight (72.7%) of them experienced moderate to severe stages of postoperative acute kidney injury. Univariable analysis showed that patients with acute kidney injury were older (difference 8 years, P < 0.001); had higher body mass index (difference 1.68 kg·m−2, P = 0.002); rates of smoking (OR = 2, P = 0.020), hypertension (OR = 2.83, P = 0.004), and renal dysfunction (OR = 3.58, P = 0.002); higher central venous pressure (difference 3 cm H2O, P < 0.001); and lower cardiac index (difference −0.23 L·min−1·m−2, P < 0.001) than patients without acute kidney injury. Multivariable regression analysis showed that advanced age (OR 1.03, P = 0.003), high body mass index (OR 1.10, P = 0.024), preoperative atrial arrhythmia (OR 3.12, P = 0.041), renal dysfunction (OR 2.70 P = 0.043), high central venous pressure (OR 1.12, P = 0.002), and low cardiac index (OR 0.36, P = 0.009) were associated with a high risk of postoperative acute kidney injury. A nomogram was established based on the regression results. The model showed good model fitness (Hosmer-Lemeshow test P = 0.881), with an area under the curve value of 0.78 (95% CI: 0.71, 0.84, P < 0.001).

Conclusion

The prediction model may help with early recognition, management, and reduction of acute kidney injury after pericardiectomy.
Correlation Between Smoking Paradox and Heart Rhythm Outcomes in Patients With Coronary Artery Disease Receiving Percutaneous Coronary Intervention
Authors: Wu Han-Ping , Jan Sheng-Ling , Chang Shih-Lin , Huang Chia-Chen , Lin Mao-Jen
Published: February 2022

Background

The effect of smoking on short-term outcomes among patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI) is controversial. However, little is known about the impact of smoking on long-term outcomes in patients with stable coronary artery disease (CAD) who receive PCI.

Methods

A total of 2,044 patients with stable CAD undergoing PCI were evaluated. They were divided into two groups according to smoking status (current smokers vs. non-smokers). Baseline characteristics, exposed risk factors, angiographic findings, and interventional strategies were assessed to compare the long-term clinical outcomes between groups. Predictors for myocardial infarction (MI), all-cause death, cardiovascular (CV) death, and repeated PCI procedures were also analyzed.

Results

Compared with non-smokers, current smokers were younger and mostly male (both P < 0.01). They also had a lower prevalence of chronic kidney disease (CKD) and diabetes (both P < 0.01). Drugs including a P2Y12 receptor inhibitor of platelets (P2Y12 inhibitor), beta-blockers (BB), and statins were used more frequently in current smokers (P < 0.01, P < 0.01, P = 0.04, respectively). Freedom from all-cause death and CV death was lower in the non-smoker group (P < 0.001, P = 0.003, respectively). After adjustment, logistic regression revealed smoking was a major predictor for all-cause death and repeated PCI procedure [hazard ratio(HR): 1.71 and 1.46, respectively].

Conclusions

Smoker's paradox extends to long-term outcome in patients with stable CAD undergoing PCI, which is partially explained by differences in baseline characteristics. However, smoking strongly predicted all-cause mortality and repeated PCI procedures in patients with stable CAD undergoing PCI.
Regression and Classification With Spline-Based Separable Expansions
Authors: Govindarajan Nithin , Vervliet Nico , De Lathauwer Lieven
Published: February 2022

We introduce a supervised learning framework for target functions that are well approximated by a sum of (few) separable terms. The framework proposes to approximate each component function by a B-spline, resulting in an approximant where the underlying coefficient tensor of the tensor product expansion has a low-rank polyadic decomposition parametrization. By exploiting the multilinear structure, as well as the sparsity pattern of the compactly supported B-spline basis terms, we demonstrate how such an approximant is well-suited for regression and classification tasks by using the Gauss–Newton algorithm to train the parameters. Various numerical examples are provided analyzing the effectiveness of the approach.
Experimental Study on Thermal Regime and Frost Jacking of Pile Foundation During Operation Period in Permafrost Regions
Authors: Shang Yunhu , Niu Fujun , Fang Jianhong , Wu Libo
Published: February 2022

The stability of a cast-in-place pile foundation in permafrost region is primarily subject to the thermal regime and tangential frost-heave forces (TFF) during the operation period. However, studies focusing on the thermal and mechanical characteristics of pile foundation during runtime are rare. To investigate the effect of pile foundation on the thermal regime and quantify the magnitude of unit tangential frost-heave forces (UTFF), a field experiment was conducted on the Qinghai–Tibet Plateau, China. Results showed that the cast-in-place pile foundation enhanced the heat exchange between the atmosphere and soil, which expanded the annual range of the surrounding ground temperature. Furthermore, the permafrost table depth was increased by 0.4–0.7 m (0.33–0.58 times the pile diameter). The TFF increased significantly when the soil temperature decreased from 0 to −0.5°C. Meanwhile, the thickness of the frost heaving layer was approximately double that of the active layer, in which the maximum UTFF was higher than 52.04 kPa. The adfreezing bond force of permafrost to pile shaft burdened most of the applied load, and the end bearing contributed relatively little. Findings from this study will benefit the stability maintenance and structure design of pile foundation in permafrost regions.
Designing Heritage Itineraries in Trainee Teachers Through Virtual Inter-University and Collaboration Groups: The Examples of Barcelona and La Laguna in Social Sciences Teaching
Authors: Farrujia de la Rosa A. José , Martínez-Gil Tània , Gómez Cristo Manuel Hernández , Sáez-Rosenkranz Isidora
Published: February 2022

In the following paper, we present some general results of an innovative teaching experience carried out by the University of La Laguna (ULL) and the University of Barcelona (UB). The project took place during a 6-week period by applying for cooperative work among early childhood (n = 109) and primary (n = 86) education trainee teachers in a selected virtual environment (ULL). One of the key aspects was the inter-university student’s grouping among both bachelor programs (early childhood and primary). The project had two main aims, which are as follows: (1) to familiarize students with heritage itineraries as key tools for the teaching of Social Sciences and (2) to develop professional competencies related to the coordination of teachers among educational levels, teaching, and learning strategies when using historical heritage and improving digital competencies. To achieve these aims, a three-phase intervention was designed (planning, design, and evaluation), all including active learning and the use of local environments as a teaching resource. To investigate the outcomes of the project, two sources of data were analyzed, namely, the results of a rubric and an opinion questionnaire (Cronbach’s α = 0.693), applied pre- (n = 185), and post- (n = 152) intervention. The descriptive statistical analysis was carried out with the Statistical Package for the Social Sciences (SPSS; v. 22) showing the positive learning outcomes of the participants. The rubric’s results showed that collaborative work and skills development scored higher, while contents of Social Sciences and the handling of heritage itineraries as teaching strategies were correct but lower than the other parameters analyzed. The questionnaire showed a positive perception of working together in consecutive educational levels and of the uses of digital technology for collaborative work but also indicated some difficulties regarding group organization, especially in the first weeks. The main results of the project highlight several aspects. (1) The need to apply pedagogical, technological, and educational resources to promote active and meaningful learning in future teachers. (2) The need to use virtual environments as learning and communication spaces in inter-university contexts, and (3) the importance of using local environments as scenarios for teaching Social Sciences.
J-Shaped Relationship Between Serum Prolactin and Metabolic-Associated Fatty Liver Disease in Female Patients With Type 2 Diabetes
Authors: Zhu Cuiling , Ma Huihui , Huang Dongdong , Li Guifang , Gao Jingyang , Cai Meili , You Hui , Bu Le , Qu Shen
Published: February 2022

Background

Metabolic-associated fatty liver disease (MAFLD) has become a worldwide epidemic. Prolactin (PRL), a pituitary hormone, has been linked to MAFLD. As a result, we set out to look into the relationship between serum PRL and the risk of MAFLD in patients with type 2 diabetes mellitus (T2DM).

Methods

A total of 724 adults with T2DM were enrolled and categorized as MAFLD and non-MAFLD groups. Anthropometric data, biochemical parameters, and serum PRL levels were collected. Liver steatosis and fibrosis were assessed using FibroScan. Patients were stratified into normal PRL (NP) and high PRL (HP) groups and divided into four groups based on serum PRL quartiles. Multivariate logistic regression analysis was performed to evaluate the association between serum PRL and MAFLD risk.

Results

Female but not male patients with MAFLD, liver steatosis, and fibrosis had significantly lower PRL levels in the NP group but higher PRL levels in the HP group than their counterparts. The proportions of MAFLD, liver steatosis, and fibrosis were significantly decreased in the NP group but increased in the HP group across the PRL quartiles in females but not in males. After multivariate adjustment, the adjusted ORs (AORs) and 95% CI for MAFLD among females were 18.165 (3.425–96.336), 1.784 (0.658–5.002), 1.744 (0.608–4.832), and 1.00 (reference) in the NP group (Q1–Q4, P-trend < 0.001) and 1.00 (reference), 11.098 (1.819–110.356), 15.225 (1.996–116.112), and 18.211 (2.579–128.568) in the HP group (Q1–Q4, P-trend = 0.020). Such associations were also found between serum PRL and liver fibrosis in females but not in males.

Conclusion

We observed a J-shaped association between serum PRL and the risk of MAFLD and liver fibrosis in females but not in males with T2DM, indicating that PRL may be relevant to MAFLD and its progression in a gender-specific manner.

Clinical Trial Registration

Chinese Clinical Trial Registry, number ChiCTR-OCS-12002381.
Financial Development, Institutional Quality, and the Influence of Various Environmental Factors on Carbon Dioxide Emissions: Exploring the Nexus in China
Authors: Amin Azka , Ameer Waqar , Yousaf Hazrat , Akbar Muhammad
Published: February 2022

Carbon dioxide (CO2) emissions have been the key source of extreme environmental degradation and have an adverse impact on climate and human activities. Although a large number of studies have explored the determinants of CO2 emissions, the role of institutional quality has not been fully studied. Our study contributes to the existing literature by examining the influence of financial development, institutional quality, foreign direct investment, trade openness, urbanization, and renewable energy consumption on CO2 emissions over the period 1996–2020 by utilizing the dynamic autoregressive distributed lag simulations. The empirical findings of the study indicate that the indicators of governance, trade, financial development, and renewable energy consumption adversely affect CO2 emissions, while urbanization and foreign direct investment contribute to environmental degradation. The empirical results of this study indicate that in order to mitigate environmental degradation and to achieve environmental sustainability, the government should establish consistency between environmental and economic policies. Moreover, in order to achieve low carbon emissions and sustainable development, countries need viable financial institutions that focus on green growth by promoting clean production process strategies to ensure the reduction of CO2 emissions.
Grouped SPME Comparison of Floral Scent as a Method of Unlocking Phylogenetic Patterns in Volatiles
Authors: Latinovic Adelina , Nichols David S. , Adams Vanessa M. , McQuillan Peter B.
Published: February 2022

Global crop production rate has exceeded the availability of pollination services provided by managed honeybees, and habitat loss remains a key factor in the loss of wild pollinators. Revegetation of agricultural land and wild pollination may provide a solution; however, the collection of floral trait data that are correlated to pollinator preferences remains an under studied and complex process. Here, we demonstrate a method for scent analysis, ordination [non-metric dimensional scaling (NMDS)], and clustering outputs that provides a fast and reproducible procedure for a broad grouping of flora based on scent and unlocking characteristic inter-floral patterns. We report the floral profiles of 15 unstudied native Australian plant species and the extent to which they match the commonly cultivated seed crops of Daucus carota L and Brassica rapa L. Through solid-phase microextraction (SPME) paired with gas chromatography–mass spectrometry (GC-MS), we identify a set of inter-family shared, common floral volatiles from these plant species as well as unique and characteristic patterns.
Less Is More: Lowering Cattle Stocking Rates Enhances Wild Herbivore Habitat Use and Cattle Foraging Efficiency
Authors: Wells Harry B. M. , Crego Ramiro D. , Ekadeli Jackson , Namoni Mathew , Kimuyu Duncan M. , Odadi Wilfred O. , Porensky Lauren M. , Dougill Andrew J. , Stringer Lindsay C. , Young Truman P.
Published: February 2022

Over a quarter of the world’s land surface is grazed by cattle and other livestock, which are replacing wild herbivores and widely regarded as drivers of global biodiversity declines. The effects of livestock presence versus absence on wild herbivores are well documented. However, the environmental context-specific effects of cattle stocking rate on biodiversity and livestock production are poorly understood, precluding nuanced rangeland management recommendations. To address this, we used a long term exclosure experiment in a semi-arid savanna ecosystem in central Kenya that selectively excludes cattle (at different stocking rates), wild mesoherbivores, and megaherbivores. We investigated the individual and interactive effects of cattle stocking rate (zero/moderate/high) and megaherbivore (>1,000 kg) accessibility on habitat use (measured as dung density) by two dominant wild mesoherbivores (50–1,000 kg; zebraEquus quaggaand elandTaurotragus oryx) across the “wet” and “dry” seasons. To explore potential tradeoffs or co-benefits between cattle production and wildlife conservation, we tested for individual and interactive effects of cattle stocking rate and accessibility by wild mesoherbivores and megaherbivores (collectively, large wild herbivores) on the foraging efficiency of cattle across both seasons. Eland habitat use was reduced by cattle at moderate and high stocking rates across both dry and wet seasons and regardless of megaherbivore accessibility. We observed a positive effect of megaherbivores on zebra habitat use at moderate, but not high, stocking rates. Cattle foraging efficiency (g dry matter step–1min–1) was lower in the high compared to moderate stocking rate treatments during the dry season, and was non-additively reduced by wild mesoherbivores and high cattle stocking rates during the wet season. These results show that high stocking rates are detrimental to wild mesoherbivore habitat use and cattle foraging efficiency, while reducing to moderate stocking rates can benefit zebra habitat use and cattle foraging efficiency. Our findings demonstrate that ecosystem management and restoration efforts across African rangelands that involve reducing cattle stocking rates may represent a win-win for wild herbivore conservation and individual performance of livestock.
HIV-Sheltering Platelets From Immunological Non-Responders Induce a Dysfunctional Glycolytic CD4+ T-Cell Profile
Authors: Zhu Aiwei , Real Fernando , Zhu Jaja , Greffe Ségolène , de Truchis Pierre , Rouveix Elisabeth , Bomsel Morgane , Capron Claude
Published: February 2022

Immunological non-responders (InRs) are HIV-infected individuals in whom the administration of combination antiretroviral therapy (cART), although successful in suppressing viral replication, cannot properly reconstitute patient circulating CD4+ T-cell number to immunocompetent levels. The causes for this immunological failure remain elusive, and no therapeutic strategy is available to restore a proper CD4+ T-cell immune response in these individuals. We have recently demonstrated that platelets harboring infectious HIV are a hallmark of InR, and we now report on a causal connection between HIV-containing platelets and T-cell dysfunctions. We show here that in vivo, platelet–T-cell conjugates are more frequent among CD4+ T cells in InRs displaying HIV-containing platelets (<350 CD4+ T cells/μl blood for >1 year) as compared with healthy donors or immunological responders (IRs; >350 CD4+ T cells/μl). This contact between platelet containing HIV and T cell in the conjugates is not infectious for CD4+ T cells, as coculture of platelets from InRs containing HIV with healthy donor CD4+ T cells fails to propagate infection to CD4+ T cells. In contrast, when macrophages are the target of platelets containing HIV from InRs, macrophages become infected. Differential transcriptomic analyses comparing InR and IR CD4+ T cells reveal an upregulation of genes involved in both aerobic and anaerobic glycolysis in CD4+ T cells from InR vs. IR individuals. Accordingly, InR platelets containing HIV induce a dysfunctional increase in glycolysis-mediated energy production in CD4+ T cells as compared with T cells cocultured with IR platelets devoid of virus. In contrast, macrophage metabolism is not affected by platelet contact. Altogether, this brief report demonstrates a direct causal link between presence of HIV in platelets and T-cell dysfunctions typical of InR, contributing to devise a platelet-targeted therapy for improving immune reconstitution in these individuals.
Hypoxia Promotes Neutrophil Survival After Acute Myocardial Infarction
Authors: Dölling Maximilian , Eckstein Markus , Singh Jeeshan , Schauer Christine , Schoen Janina , Shan Xiaomei , Bozec Aline , Knopf Jasmin , Schett Georg , Muñoz Luis E. , Herrmann Martin
Published: February 2022

Phagocytosis, degranulation, and neutrophil extracellular traps (NETs) formation build the armory of neutrophils for the first line of defense against invading pathogens. All these processes are modulated by the microenvironment including tonicity, pH and oxygen levels. Here we investigated the neutrophil infiltration in cardiac tissue autopsy samples of patients with acute myocardial infarction (AMI) and compared these with tissues from patients with sepsis, endocarditis, dermal inflammation, abscesses and diseases with prominent neutrophil infiltration. We observed many neutrophils infiltrating the heart muscle after myocardial infarction. Most of these had viable morphology and only few showed signs of nuclear de-condensation, a hallmark of early NET formation. The abundance of NETs was the lowest in acute myocardial infarction when compared to other examined diseases. Since cardiac oxygen supply is abruptly abrogated in acute myocardial infarction, we hypothesized that the resulting tissue hypoxia increased the longevity of the neutrophils. Indeed, the viable cells showed increased nuclear hypoxia inducible factor-1α (HIF-1α) content, and only neutrophils with low HIF-1α started the process of NET formation (chromatin de-condensation and nuclear swelling). Prolonged neutrophil survival, increased oxidative burst and reduced NETs formation were reproduced under low oxygen tensions and by HIF-1α stabilization in vitro. We conclude that nuclear HIF-1α is associated with prolonged neutrophil survival and enhanced oxidative stress in hypoxic areas of AMI.
Dental and Orthopaedic Implant Loosening: Overlap in Gene Expression Regulation
Authors: Schluessel Sabine , Hartmann Eliza S. , Koehler Miriam I. , Beck Felicitas , Redeker Julia I. , Saller Maximilian M. , Akova Elif , Krebs Stefan , Holzapfel Boris M. , Mayer-Wagner Susanne
Published: February 2022

Objectives

Endoprosthetic loosening still plays a major role in orthopaedic and dental surgery and includes various cellular immune processes within peri-implant tissues. Although the dental and orthopaedic processes vary in certain parts, the clinical question arises whether there are common immune regulators of implant loosening. Analyzing the key gene expressions common to both processes reveals the mechanisms of osteoclastogenesis within periprosthetic tissues of orthopaedic and dental origin.

Methods

Donor peripheral blood mononuclear cells (PBMCs) and intraoperatively obtained periprosthetic fibroblast-like cells (PPFs) were (co-)cultured with [± macrophage-colony stimulating factor (MCSF) and Receptor Activator of NF-κB ligand (RANKL)] in transwell and monolayer culture systems and examined for osteoclastogenic regulations [MCSF, RANKL, osteoprotegerin (OPG), and tumor necrosis factor alpha (TNFα)] as well as the ability of bone resorption. Sequencing analysis compared dental and orthopaedic (co-)cultures.

Results

Monolayer co-cultures of both origins expressed high levels of OPG, resulting in inhibition of osteolysis shown by resorption assay on dentin. The high OPG-expression, low RANKL/OPG ratios and a resulting inhibition of osteolysis were displayed by dental and orthopaedic PPFs in monolayer even in the presence of MCSF and RANKL, acting as osteoprotective and immunoregulatory cells. The osteoprotective function was only observed in monolayer cultures of dental and orthopaedic periprosthetic cells and downregulated in the transwell system. In transwell co-cultures of PBMCs/PPFs profound changes of gene expression, with a significant decrease of OPG (20-fold dental versus 100 fold orthopaedic), were identified. Within transwell cultures, which offer more in vivo like conditions, RANKL/OPG ratios displayed similar high levels to the original periprosthetic tissue. For dental and orthopaedic implant loosening, overlapping findings in principal component and heatmap analysis were identified.

Conclusions

Thus, periprosthetic osteoclastogenesis may be a correlating immune process in orthopaedic and dental implant failure leading to comparable reactions with regard to osteoclast formation. The transwell cultures system may provide an in vivo like model for the exploration of orthopaedic and dental implant loosening.
Role of Serine Proteases at the Tumor-Stroma Interface
Authors: Tagirasa Ravichandra , Yoo Euna
Published: February 2022

During tumor development, invasion and metastasis, the intimate interaction between tumor and stroma shapes the tumor microenvironment and dictates the fate of tumor cells. Stromal cells can also influence anti-tumor immunity and response to immunotherapy. Understanding the molecular mechanisms that govern this complex and dynamic interplay, thus is important for cancer diagnosis and therapy. Proteolytic enzymes that are expressed and secreted by both cancer and stromal cells play important roles in modulating tumor-stromal interaction. Among, several serine proteases such as fibroblast activation protein, urokinase-type plasminogen activator, kallikrein-related peptidases, and granzymes have attracted great attention owing to their elevated expression and dysregulated activity in the tumor microenvironment. This review highlights the role of serine proteases that are mainly derived from stromal cells in tumor progression and associated theranostic applications.
Human CD4 T Cells From Thymus and Cord Blood Are Convertible Into CD8 T Cells by IL-4
Authors: Nunes-Cabaço Helena , Ramalho-dos-Santos Andreia , Pires Ana R. , Martins Leila R. , Barata João T. , Sousa Ana E.
Published: February 2022

Commitment to the CD4+ or CD8+ T cell lineages is linked to the acquisition of a functional program broadly defined by helper and cytotoxic properties, respectively. The mechanisms underlying these processes in the human thymus remain largely unclear. Moreover, recent thymic emigrants are thought to have some degree of plasticity, which may be important for the shaping of the immune system and adjustment to specific peripheral needs. We show here that IL-4 induces proliferation-independentde novosynthesis of CD8αβ in human CD4 single-positive (SP) thymocytes, generating a stable CD8SP population that features a diverse TCRαβ repertoire, CD4 expression shut-down and ThPOK downregulation. IL-4 also promotes an innate-like program in both CD4SP and CD8SP thymocytes, characterized by Eomes upregulation in the absence of T-bet, in line with its recognized role in the generation of thymic innate-like CD8+ T cells. The clinical relevance of these findings is further supported by the profile of IL-4 production and IL-4 receptor expression that we identified in the human thymus. Importantly, human cord blood CD4+ T cells preserve the ability to generate Eomes+ CD8+ T cells in the presence of IL-4, with implications in neonatal immunity. Our results support a role for IL-4 in the dynamic regulation of human thymocyte plasticity and identify novel strategies to modulate immune responses.
Localized Intra-Cavitary Therapy to Drive Systemic Anti-Tumor Immunity
Authors: Donnenberg Vera S. , Wagner Patrick L. , Luketich James D. , Bartlett David L. , Donnenberg Albert D.
Published: February 2022

Metastasis to the pleural and peritoneal cavities is a common terminal pathway for a wide variety of cancers. This article explores how these unique environments both promote aggressive tumor behavior and suppresses anti-tumor immunity, and ways in which local delivery of protein therapeutics can leverage the contained nature of these spaces to a therapeutic advantage, achieving high intra-cavital concentrations while minimizing systemic toxicity.
Characterization of Geochemistry in Hydrothermal Sediments From the Newly Discovered Onnuri Vent Field in the Middle Region of the Central Indian Ridge
Authors: Lim Dhongil , Kim Jihun , Kim Wonnyon , Kim Jonguk , Kim Dongsung , Zhang Le , Kwack Kyungun , Xu Zhaokai
Published: February 2022

The recently discovered Onnuri hydrothermal vent field (OVF) is a typical off-axis ultramafic-hosted vent system, located on the summit of the dome-like ocean core complex (OCC) at a distance of ∼12 km from the ridge axis along the middle region of the Central Indian Ridge (CIR). The plume chemistry with high methane anomaly was consistent with the precursor of hydrothermal activity; however, the fundamental characteristic of the OVF system, such as the hydrothermal circulation process and source of heat, remains poorly understood. Here, we focus on the geochemical features of surface sediments and minerals collected at and around the OVF region in order to better understand this venting system. The results reveal that the OVF sediments are typified by remarkably high concentrations of Fe, Si, Ba, Cu, and Zn, derived from hydrothermal fluid and S and Mg from seawater; depleted C-S isotope compositions; and abundant hydrothermally precipitated minerals (i.e., Fe–Mn hydroxides, sulfide and sulfate minerals, and opal silica). Notably, the occurrence of pure talc and barite bears witness to strong hydrothermal activity in the OVF, and their sulfur and strontium isotope geochemistry agree with extensive mixing of the unmodified seawater with high-temperature fluid derived from the gabbroic rock within the ultramafic-dominated ridge segment. The findings reveal that the OVF is a representative example of an off-axis, high-temperature hydrothermal circulation system, possibly driven by the exothermic serpentinization of exposed peridotites. Given the widespread distribution of OCC with detachment faults, furthermore, the OVF may be the most common type of hydrothermal activity in the CIR, although the paucity of data precludes generalizing this result. This study provides important information contributing to our understanding of the ultramafic-hosted hydrothermal vent system with a non-magmatic heat source along mid-ocean ridges.
Aquaporins in Pacific White Shrimp (Litopenaeus vannamei): Molecular Characterization, Expression Patterns, and Transcriptome Analysis in Response to Salinity Stress
Authors: Wang Zhongkai , Chen Yigeng , Wang Cong , Zhao Nannan , Zhang Zhihao , Deng Zhitong , Cui Yanting , Wang Renjie , Li Yuquan
Published: February 2022

Aquaporins (AQPs) are integral membrane proteins that facilitate the transport of water and small solutes across cell membranes. These proteins are vital for maintaining water homeostasis in living organisms. In mammals, thirteen aquaporins have been characterized, but in crustaceans, especially penaeid shrimp, the diversity, structure, and substrate specificity of these membrane channel proteins are largely unknown. We here presented the three types of AQPs from Litopenaeus vannamei based on genome and transcriptome sequencing. Phylogenetic analysis showed that each AQP separately represented members of aquaglyceroporins, classical aquaporins, and unorthodox aquaporins, thus they were named as LvAQP3, LvAQP4, and LvAQP11. The LvAqp4 was mostly expressed in hepatopancreas, stomach, and gill, meanwhile LvAqp3 and LvAqp11 were separately predominantly expressed in intestine and muscle, respectively. To investigate possible roles of aquaporins in osmoregulation, mRNA expression changes in mainly expressed tissues were analyzed after acute exposure or long-term acclimation to different salinities. The results revealed that the expression levels of aquaporins genes were significantly decreased in most tissues (except hepatopancreas) under salinity stress, though the expression patterns were variable among isoforms and tissues. Moreover, comparative transcriptome analysis indicated the combination roles of aquaglyceroporin and amino acid metabolism related genes and pathways in response to acute salinity changes in the intestine. This study opened new windows for future investigations and provided new insights into the role of aquaporins in osmoregulation in L. vannamei.
Accurate Sensorless Multistable Microsystem With a Single Actuator
Authors: Hussein Hussein , Fariborzi Hossein
Published: February 2022

A compact and monolithic multistable microsystem was developed to realize the accurate positioning on the microscale by using a single actuator, without any positioning sensors. The mobile part can be maintained in several stable positions at rest with accurate intermediate steps and a high stability margin at each position. Switching between stable positions can be performed in both directions on one axis by using a single actuator. Using the mechanical intelligence encoded in the internal mechanisms, the mobile part position can be switched one step forward or back to the initial position through a single activation of the actuator. Multistable devices were designed and fabricated based on the working principle, and their satisfactory operation, with effective holding at stable positions and switching in two directions, was experimentally demonstrated.
Short-Course of Methylprednisolone Improves Respiratory Functional Parameters After 120 Days in Hospitalized COVID-19 Patients (Metcovid Trial): A Randomized Clinical Trial
Authors: Barros Camila Miriam Suemi Sato , Freire Raissa Soares , Frota Elisângela , Rezende Santos Anna Gabriela , Farias Maria Eduarda Leão , Rodrigues Maria Gabriela Almeida , Silva Bernardo Maia , Prado Jeronimo Christiane Maria , Netto Rebeca Linhares Abreu , Silva Borba Mayla Gabriela , Baía-da-Silva Djane , Brito-Sousa José Diego , Xavier Mariana Simão , Araújo-Alexandre Marcia Almeida , Sampaio Vanderson Souza , Melo Gisely Cardoso , Arêas Guilherme Tinoco , Hajjar Ludhmila Abrahão , Monteiro Wuelton Marcelo , Gomes Naveca Felipe , Costa Fábio Trindade Maranhão , Val Fernando Fonseca Almeida , Lacerda Marcus Vinícius Guimarães ,   The Metcovid team
Published: February 2022

Background: The use of corticosteroids may help control the cytokine storm occurring in acute respiratory failure due to the severe form of COVID-19. We evaluated the postacute effect of corticosteroids used during the acute phase, such as impairment in pulmonary function parameters, on day 120 (D120)-follow-up, in participants who survived over 28 days.

Methods: This is a parallel, double-blind, randomized, placebo-controlled phase IIb clinical trial carried out between April 18 and October 9, 2020, conducted in hospitalized patients with clinical–radiological suspicion of COVID-19, aged 18 years or older, with SpO2 ≤ 94% on room air or requiring supplementary oxygen, or under invasive mechanical ventilation (IMV) in a referral center in Manaus, Western Brazilian Amazon. Intravenous methylprednisolone (MP) (0.5 mg/kg) was given two times daily for 5 days to these patients. The primary outcome used for this study was pulmonary function testing at day 120 follow-up visit.

Results: Out of the total of surviving patients at day 28 (n = 246) from the Metcovid study, a total of 118 underwent satisfactory pulmonary function testing (62 in the placebo arm and 56 in the MP arm). The supportive treatment was similar between the placebo and MP groups (seven [11%] vs. four [7%]; P = 0.45). At hospital admission, IL-6 levels were higher in the MP group (P < 0.01). Also, the need for ICU (P = 0.06), need for IMV (P = 0.07), and creatine kinase (P = 0.05) on admission also tended to be higher in this group. In the univariate analysis, forced expiratory volume on 1st second of exhalation (FEV1) and forced vital capacity (FVC) at D120 follow-up were significantly higher in patients in the MP arm, being this last parameter also significantly higher in the multivariate analysis independently of IMV and IL-6 levels on admission.

Conclusion: The use of steroids for at least 5 days in severe COVID-19 was associated with a higher FVC, which suggests that hospitalized COVID-19 patients might benefit from the use of MP in its use in the long-term, with less pulmonary restrictive functions, attributed to fibrosis.

Trial Registration:ClinicalTrials.gov, Identifier: NCT04343729.
Complementing Tissue Testing With Plasma Mutation Profiling Improves Therapeutic Decision-Making for Patients With Lung Cancer
Authors: Choudhury Yukti , Tan Min-Han , Shi Jun Li , Tee Augustine , Ngeow Kao Chin , Poh Jonathan , Goh Ruth Rosalyn , Mong Jamie
Published: February 2022

Background

Tissue biopsy is an integral part of the diagnostic approach to lung cancer. It is however invasive and limited by heterogeneity. Liquid biopsies may complement tissue testing by providing additional molecular information and may be particularly helpful in patients from whom obtaining sufficient tissue for genomic profiling is challenging.

Methods

Patients with suspected lung cancer (n = 71) were prospectively recruited. Blood and diagnostic tissue samples were collected within 48 h of each other. Plasma cell-free DNA (cfDNA) testing was done using an ultrasensitive amplicon-based next-generation sequencing (NGS) panel (plasma NGS testing). For cases diagnosed as non-small cell lung carcinoma (NSCLC) via histology or cytology, targeted testing for epidermal growth factor receptor (EGFR) mutations was performed using tissue biopsy samples (tissue EGFR testing), where available. Concordance of clinically actionable mutations between methods and sample types was assessed.

Results

For confirmed NSCLC cases (n = 54), tissue EGFR test results were available only for 70.3% (38/54) due to sample inadequacies, compared to blood samples for 98.1% (53/54) cases. Tissue EGFR testing identified sensitizing EGFR (L858R or exon 19 deletion) mutation in 31.6% (12/38) of cases. Plasma NGS identified clinically actionable mutations in 37.7% (20/53) of cases, including EGFR mutations in two cases with no tissue EGFR results, and mutations in KRAS, BRAF, and MET. The overall sensitivity of sensitizing EGFR mutation detection by plasma NGS was 75% (9/12), and specificity was 100% (25/25) in patients tested in both tissue EGFR and plasma NGS (n = 37). In this cohort of patients, tissue EGFR testing alone informed clinical decisions in 22.2% (12/54) of cases. Adding plasma NGS to tissue EGFR testing increased the detection rate of actionable mutations to 42.6% (23/54), representing a 1.9-fold increase in clinically relevant findings. The average turnaround time of plasma NGS was shorter than standard tissue testing (10 vs. 29.9 days, p < 0.05).

Conclusions

In the first-line setting, plasma NGS was highly concordant with tissue EGFR testing. Plasma NGS increases the detection of actionable findings with a shorter time to results. This study outlines the clinical utility of complementary plasma mutation profiling in the routine management of lung cancer patients.
DIC Score Combined With CLIF-C OF Score Is More Effective in Predicting Prognosis in Patients With Hepatitis B Virus Acute-on-Chronic Liver Failure
Authors: Zhang Xueyun , Han Jiajia , Qi Xun , Zhang Yao , Zhou Pu , Liu Xiaoqin , Ying Yue , Zhang Wenhong , Zhang Jiming , Huang Yuxian
Published: February 2022

Coagulation and fibrinolysis disorders are major prognostic factors in hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) patients. Here, we aimed to clarify the role of disseminated intravascular coagulation (DIC) scores in predicting HBV-ACLF patient prognosis. We assessed the DIC score from HBV-ACLF patients at Huashan Hospital in Shanghai, China from June 2013 to May 2021 and evaluated it in relation to short-term mortality, clinical course, and infection. A novel prognostic scoring model was proposed based on DIC scores. A total of 163 transplant-free HBV-ACLF patients were enrolled. DIC scores were higher in non-survivors than survivors (6 vs. 4, P = 0.000) and were independently associated with short-term mortality [hazard ratio (HR): 1.397, 95% confidence interval (95% CI): 1.040–1.875, P = 0.026]. DIC scores were associated with ACLF grade, clinical course, and infection. Moreover, they were correlated with model for end-stage liver disease (MELD) scores (r = 0.521, P < 0.001). The area under the receiver operating curve (auROC) of CLIF-C OF-DICs [a novel prognostic score based on age, DIC score, and Chronic liver failure-consortium organ function score (CLIF-C OFs)] for 90-day mortality was 0.936, which was higher than six other generic prognostic scoring models. These results were confirmed in a validation cohort (n = 82). In conclusion, elevated DIC score is associated with poor prognosis in HBV-ACLF patients, and can be used jointly with CLIF-C OFs to improve the accuracy of prognosis prediction.
Blood Group Testing
Authors: Li Hong-Yang , Guo Kai
Published: February 2022

Red blood cell (RBC) transfusion is one of the most frequently performed clinical procedures and therapies to improve tissue oxygen delivery in hospitalized patients worldwide. Generally, the cross-match is the mandatory test in place to meet the clinical needs of RBC transfusion by examining donor-recipient compatibility with antigens and antibodies of blood groups. Blood groups are usually an individual's combination of antigens on the surface of RBCs, typically of the ABO blood group system and the RH blood group system. Accurate and reliable blood group typing is critical before blood transfusion. Serological testing is the routine method for blood group typing based on hemagglutination reactions with RBC antigens against specific antibodies. Nevertheless, emerging technologies for blood group testing may be alternative and supplemental approaches when serological methods cannot determine blood groups. Moreover, some new technologies, such as the evolving applications of blood group genotyping, can precisely identify variant antigens for clinical significance. Therefore, this review mainly presents a clinical overview and perspective of emerging technologies in blood group testing based on the literature. Collectively, this may highlight the most promising strategies and promote blood group typing development to ensure blood transfusion safety.
Proposal of Two New Combinations, Twenty New Species, Four New Genera, One New Family, and One New Order for the Anamorphic Basidiomycetous Yeast Species in Ustilaginomycotina
Authors: Li Yao-Yao , Wang Man-Man , Groenewald Marizeth , Li Ai-Hua , Guo Yun-Tong , Wu Feng , Zhang Bing-Qian , Tanaka Eiji , Wang Qi-Ming , Bai Feng-Yan , Begerow Dominik
Published: February 2022

Two hundred and forty-four ustilaginomycetous yeast or yeast-like strains were isolated from the soil, skin of animals or humans and plant materials during the past 20 years. Among them, 203 strains represent 39 known species, whereas 41 strains represent several novel species based on the sequence analyses of the rDNA genes [18S rDNA, Internal Transcribed Spacer (ITS) regions, 26S rDNA D1/D2 domain] and three protein genes (RPB1, RPB2, and TEF1). In this study, one new order, one new family, four new genera, twenty new species, and two new combinations were proposed. They are Franziozymales ord. nov., Franziozymaceae fam. nov., Baueromyces gen. nov., Franziozyma gen. nov., Guomyces gen. nov., Yunzhangomyces gen. nov., Baueromyces planticola sp. nov., Franziozyma bambusicola sp. nov., Gjaerumia cyclobalanopsidis sp. nov., Gjaerumia pseudominor sp. nov., Jamesdicksonia aceris sp. nov., Jaminaea lantanae sp. nov., Kalmanozyma hebeiensis sp. nov., Langdonia ligulariae sp. nov., Meira hainanensis sp. nov., Meira pileae sp. nov., Meira plantarum sp. nov., Phragmotaenium parafulvescens sp. nov., Sporisorium cylindricum sp. nov., Sympodiomycopsis europaea sp. nov., Tilletiopsis lunata sp. nov., Tilletiopsis pinicola sp. nov., Yunzhangomyces clavatus sp. nov., Yunzhangomyces cylindricus sp. nov., Yunzhangomyces qinlingensis sp. nov., Yunzhangomyces orchidis sp. nov., Guomyces nicotianae comb. nov., and Yunzhangomces scirpi comb. nov.
Exploring the Addition of Herbal Residues on Fermentation Quality, Bacterial Communities, and Ruminal Greenhouse Gas Emissions of Paper Mulberry Silage
Authors: Li Xiaomei , Chen Fei , Xu Jingjing , Guo Linna , Xiong Yi , Lin Yanli , Ni Kuikui , Yang Fuyu
Published: February 2022

This study aimed to investigate the influence of herbal residues on the fermentation quality and ruminal fermentation of paper mulberry silage. Clove, mint, and purple perilla residues were used as additives. Silage treatments were designed as control (no additives), 5% of clove, 5% of mint, and 5% of purple perilla. After 21 and 75 days of fermentation, the fermentation characteristics, bacterial communities, and ruminal greenhouse gas emissions in vitro incubation of paper mulberry were analyzed. The results showed that the used herbal residues could reduce the protein losses in paper mulberry silage based on the lower contents of ammoniacal nitrogen and nonprotein nitrogen. Compared with control, higher lactic acid and propionic acid contents were observed in the silages treated with mint and purple perilla but with a higher acetic acid content in clove treatment. Real-time sequencing technology (single-molecule real-time) revealed that Lactobacillus was the dominant bacteria in all silages at the genus level, whereas the bacterial abundance in the treated silages differed greatly from control at the species level. Lactobacillus hammesii abundance was the highest in control, whereas Lactobacillus acetotolerans was the first predominant in the treated silages. All the additives enhanced the digestibility of in vitro dry matter significantly. However, purple perilla decreased the production of total gas, methane, and carbon dioxide. The findings discussed earlier suggested that herbal residues have potential effects in improving fermentation quality, reducing protein loss, and modulating greenhouse gas emissions in the rumen of paper mulberry silage by shifting bacterial community composition.