bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Transcriptomics of Rice Genotypes with Contrasting Responses to Nitrogen Stress Reveals Genes Influencing Nitrogen Uptake through the Regulation of Root Architecture
Authors: Subudhi Prasanta K. , Garcia Richard S. , Coronejo Sapphire , Tapia Ronald
Published: August 2020

The indiscriminate use of nitrogenous fertilizers continues unabated for commercial crop production, resulting in air and water pollution. The development of rice varieties with enhanced nitrogen use efficiency (NUE) will require a thorough understanding of the molecular basis of a plant’s response to low nitrogen (N) availability. The global expression profiles of root tissues collected from low and high N treatments at different time points in two rice genotypes, Pokkali and Bengal, with contrasting responses to N stress and contrasting root architectures were examined. Overall, the number of differentially expressed genes (DEGs) in Pokkali (indica) was higher than in Bengal (japonica) during low N and early N recovery treatments. Most low N DEGs in both genotypes were downregulated whereas early N recovery DEGs were upregulated. Of these, 148 Pokkali-specific DEGs might contribute to Pokkali’s advantage under N stress. These DEGs included transcription factors and transporters and were involved in stress responses, growth and development, regulation, and metabolism. Many DEGs are co-localized with quantitative trait loci (QTL) related to root growth and development, chlorate-resistance, and NUE. Our findings suggest that the superior growth performance of Pokkali under low N conditions could be due to the genetic differences in a diverse set of genes influencing N uptake through the regulation of root architecture.
Potential Mechanisms of Bisphenol A (BPA) Contributing to Human Disease
Authors: Cimmino Ilaria , Fiory Francesca , Perruolo Giuseppe , Miele Claudia , Beguinot Francesco , Formisano Pietro , Oriente Francesco
Published: August 2020

Bisphenol A (BPA) is an organic synthetic compound serving as a monomer to produce polycarbonate plastic, widely used in the packaging for food and drinks, medical devices, thermal paper, and dental materials. BPA can contaminate food, beverage, air, and soil. It accumulates in several human tissues and organs and is potentially harmful to human health through different molecular mechanisms. Due to its hormone-like properties, BPA may bind to estrogen receptors, thereby affecting both body weight and tumorigenesis. BPA may also affect metabolism and cancer progression, by interacting with GPR30, and may impair male reproductive function, by binding to androgen receptors. Several transcription factors, including PPARγ, C/EBP, Nrf2, HOX, and HAND2, are involved in BPA action on fat and liver homeostasis, the cardiovascular system, and cancer. Finally, epigenetic changes, such as DNA methylation, histones modification, and changes in microRNAs expression contribute to BPA pathological effects. This review aims to provide an extensive and comprehensive analysis of the most recent evidence about the potential mechanisms by which BPA affects human health.
Vascular Remodeling in Moyamoya Angiopathy: From Peripheral Blood Mononuclear Cells to Endothelial Cells
Authors: Tinelli Francesca , Nava Sara , Arioli Francesco , Bedini Gloria , Scelzo Emma , Lisini Daniela , Faragò Giuseppe , Gioppo Andrea , Ciceri Elisa F. , Acerbi Francesco , Ferroli Paolo , Vetrano Ignazio G. , Esposito Silvia , Saletti Veronica , Pantaleoni Chiara , Zibordi Federica , Nardocci Nardo , Zedde Maria Luisa , Pezzini Alessandro , Di Lazzaro Vincenzo , Capone Fioravante , Dell’Acqua Maria Luisa , Vajkoczy Peter , Tournier-Lasserve Elisabeth , Parati Eugenio A. , Bersano Anna , Gatti Laura
Published: August 2020

The pathophysiological mechanisms of Moyamoya angiopathy (MA), which is a rare cerebrovascular condition characterized by recurrent ischemic/hemorrhagic strokes, are still largely unknown. An imbalance of vasculogenic/angiogenic mechanisms has been proposed as one possible disease aspect. Circulating endothelial progenitor cells (cEPCs) have been hypothesized to contribute to vascular remodeling of MA, but it remains unclear whether they might be considered a disease effect or have a role in disease pathogenesis. The aim of the present study was to provide a morphological, phenotypical, and functional characterization of the cEPCs from MA patients to uncover their role in the disease pathophysiology. cEPCs were identified from whole blood as CD45dimCD34+CD133+ mononuclear cells. Morphological, biochemical, and functional assays were performed to characterize cEPCs. A significant reduced level of cEPCs was found in blood samples collected from a homogeneous group of adult (mean age 46.86 ± 11.7; 86.36% females), Caucasian, non-operated MA patients with respect to healthy donors (HD; p = 0.032). Since no difference in cEPC characteristics and functionality was observed between MA patients and HD, a defective recruitment mechanism could be involved in the disease pathophysiology. Collectively, our results suggest that cEPC level more than endothelial progenitor cell (EPC) functionality seems to be a potential marker of MA. The validation of our results on a larger population and the correlation with clinical data as well as the use of more complex cellular model could help our understanding of EPC role in MA pathophysiology.
Application of Mesenchymal Stem Cell Therapy and Inner Ear Regeneration for Hearing Loss: A Review
Authors: Kanzaki Sho , Toyoda Masashi , Umezawa Akihiro , Ogawa Kaoru
Published: August 2020

Inner and middle ear disorders are the leading cause of hearing loss, and are said to be among the greatest risk factors of dementia. The use of regenerative medicine for the treatment of inner ear disorders may offer a potential alternative to cochlear implants for hearing recovery. In this paper, we reviewed recent research and clinical applications in middle and inner ear regeneration and cell therapy. Recently, the mechanism of inner ear regeneration has gradually been elucidated. “Inner ear stem cells,” which may be considered the precursors of various cells in the inner ear, have been discovered in the cochlea and vestibule. Research indicates that cells such as hair cells, neurons, and spiral ligaments may form promising targets for inner ear regenerative therapies by the transplantation of stem cells, including mesenchymal stem cells. In addition, it is necessary to develop tests for the clinical monitoring of cell transplantation. Real-time imaging techniques and hearing rehabilitation techniques are also being investigated, and cell therapy has found clinical application in cochlear implant techniques.
Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to Blumeria graminis f. sp. tritici in Wheat
Authors: Hu Yuting , Zhong Shengfu , Zhang Min , Liang Yinping , Gong Guoshu , Chang Xiaoli , Tan Feiquan , Yang Huai , Qiu Xiaoyan , Luo Liya , Luo Peigao
Published: August 2020

Photosynthesis is not only a primary generator of reactive oxygen species (ROS) but also a component of plant defence. To determine the relationships among photosynthesis, ROS, and defence responses to powdery mildew in wheat, we compared the responses of the Pm40-expressing wheat line L658 and its susceptible sister line L958 at 0, 6, 12, 24, 48, and 72 h post-inoculation (hpi) with powdery mildew via analyses of transcriptomes, cytology, antioxidant activities, photosynthesis, and chlorophyll fluorescence parameters. The results showed that H2O2 accumulation in L658 was significantly greater than that in L958 at 6 and 48 hpi, and the enzymes activity and transcripts expression of peroxidase and catalase were suppressed in L658 compared with L958. In addition, the inhibition of photosynthesis in L658 paralleled the global downregulation of photosynthesis-related genes. Furthermore, the expression of the salicylic acid-related genes non-expressor of pathogenesis related genes 1 (NPR1), pathogenesis-related 1 (PR1), and pathogenesis-related 5 (PR5) was upregulated, while the expression of jasmonic acid- and ethylene-related genes was inhibited in L658 compared with L958. In conclusion, the downregulation of photosynthesis-related genes likely led to a decline in photosynthesis, which may be combined with the inhibition of peroxidase (POD) and catalase (CAT) to generate two stages of H2O2 accumulation. The high level of H2O2, salicylic acid and PR1 and PR5 in L658 possible initiated the hypersensitive response.
Design, Engineering and Discovery of Novel α-Helical and β-Boomerang Antimicrobial Peptides against Drug Resistant Bacteria
Authors: Bhattacharjya Surajit , Straus Suzana K.
Published: August 2020

In an era where the pipeline of new antibiotic development is drying up, the continuous rise of multi-drug resistant (MDR) and extensively drug resistant (XDR) bacteria are genuine threats to human health. Although antimicrobial peptides (AMPs) may serve as promising leads against drug resistant bacteria, only a few AMPs are in advanced clinical trials. The limitations of AMPs, namely their low in vivo activity, toxicity, and poor bioavailability, need to be addressed. Here, we review engineering of frog derived short α-helical AMPs (aurein, temporins) and lipopolysaccharide (LPS) binding designed β-boomerang AMPs for further development. The discovery of novel cell selective AMPs from the human proprotein convertase furin is also discussed.
Erratum: Skowron Volponi, M. A Vivid Orange New Genus and Species of Braconid-Mimicking Clearwing Moth (Lepidoptera: Sesiidae) Found Puddling on Plecoptera Exuviae. Insects 2020, 11, 425
Authors: Skowron Volponi Marta
Published: August 2020

The author wishes to make the following corrections to this paper [...]
Effects of 12-Week Methylphenidate Treatment on Neurometabolism in Adult Patients with ADHD: The First Double-Blind Placebo-Controlled MR Spectroscopy Study
Authors: Maier Simon , Tebartz van Elst Ludger , Philipsen Alexandra , Lange Thomas , Feige Bernd , Glauche Volkmar , Nickel Kathrin , Matthies Swantje , Alm Barbara , Sobanski Esther , Domschke Katharina , Perlov Evgeniy , Endres Dominique
Published: August 2020

Attention deficit hyperactivity disorder (ADHD) is a frequent neurodevelopmental disorder that often persists into adulthood. Methylphenidate (MPH) is the first-line treatment for ADHD; however, despite its wide usage, little is known about its neurometabolic effects. Until now, no randomized and blinded clinical trials have been conducted addressing the neurometabolic signals of MPH administration in adults with ADHD. In the current study, the authors investigated how MPH intake and group psychotherapy (GPT) influence brain neurometabolism over the course of three months. The authors hypothesized a decrease in the anterior cingulate cortex (ACC) glutamate concentration following MPH administration. This study was part of a double-blind multicenter trial (Comparison of Methylphenidate and Psychotherapy in Adult ADHD Study (COMPAS)) investigating the effects of MPH and GPT in patients with adult ADHD. Using single-voxel magnetic resonance spectroscopy (MRS) of the pregenual ACC and the left cerebellar hemisphere (CHL), we investigated the concentration of glutamate plus glutamine (Glx), N-acetyl-aspartate, creatine, total choline containing compounds, and myo-inositol in patients before and after 12 weeks of treatment. Neither MPH nor GPT significantly influenced the Glx concentration or any of the other metabolite concentrations in the ACC and CHL after 12 weeks. Therefore, contrary to the hypothesis, no change in the prefrontal Glx signal was detected after MPH treatment. Given that MRS does not differentiate between glutamate in the synaptic cleft and in neuronal tissue, MPH-induced down-regulation of glutamatergic neurotransmission in the ACC might only affect the concentration of glutamate in the synaptic cleft, while the general availability of glutamate in the respective neuronal tissue might be unaffected by MPH intake. The observed lack of any MPH-induced normalization in metabolite concentrations is less surprising, considering that the baseline sample did not significantly differ from a healthy control group. Future studies of other regions, such as the basal ganglia, and the use of novel methods, such as whole brain MRS and multimodal imaging approaches, are necessary.
A Computational Study of Executive Dysfunction in Amyotrophic Lateral Sclerosis
Authors: Steinke Alexander , Lange Florian , Seer Caroline , Petri Susanne , Kopp Bruno
Published: August 2020

Executive dysfunction is a well-documented, yet nonspecific corollary of various neurological diseases and psychiatric disorders. Here, we applied computational modeling of latent cognition for executive control in amyotrophic lateral sclerosis (ALS) patients. We utilized a parallel reinforcement learning model of trial-by-trial Wisconsin Card Sorting Test (WCST) behavior. Eighteen ALS patients and 21 matched healthy control participants were assessed on a computerized variant of the WCST (cWCST). ALS patients showed latent cognitive symptoms, which can be characterized as bradyphrenia and haphazard responding. A comparison with results from a recent computational Parkinson’s disease (PD) study (Steinke et al., 2020, J Clin Med) suggests that bradyphrenia represents a disease-nonspecific latent cognitive symptom of ALS and PD patients alike. Haphazard responding seems to be a disease-specific latent cognitive symptom of ALS, whereas impaired stimulus-response learning seems to be a disease-specific latent cognitive symptom of PD. These data were obtained from the careful modeling of trial-by-trial behavior on the cWCST, and they suggest that computational cognitive neuropsychology provides nosologically specific indicators of latent facets of executive dysfunction in ALS (and PD) patients, which remain undiscoverable for traditional behavioral cognitive neuropsychology. We discuss implications for neuropsychological assessment, and we discuss opportunities for confirmatory computational brain imaging studies.
Unraveling Misunderstandings about Desertification: The Paradoxical Case of the Tabernas-Sorbas Basin in Southeast Spain
Authors: Martínez-Valderrama Jaime , Guirado Emilio , Maestre Fernando
Published: August 2020

From its origins, the concept of desertification has been controversial. The prevailing confusion between two desertification visions, one that considers it as the expansion of deserts and another that emphasizes its anthropogenic component, has been transferred to society. Here we illustrate misunderstandings about desertification using a very illustrative case from the Tabernas-Sorbas Basin (Almeria, Spain), where striking badlands that are often used as an image of desertification coexist with an intensive olive agriculture that is irreversibly deteriorating the only oasis in continental Europe (Los Molinos spring). The olive tree is a traditional Mediterranean dryland crop and until the 1950s only about 200 ha were irrigated in this area. However, the profitability of the crop has caused irrigation to expand to 4400 ha in the last two decades. The process of intensification has been reinforced giving way to super-intensive irrigation, which involves going from 210 to 1550 trees/ha, which in a few years already occupies more than 1500 ha. The effects on the water balance of the aquifer feeding these crops have been severe, and the flow of the Los Molinos spring has gone from more than 40 L/s for the period 1970–2000 to the current 7.28 L/s. Unraveling the mechanisms of land degradation and its main drivers are the first step to propose management actions to achieve a more sustainable use of resources and to combat desertification.
In-Memory Logic Operations and Neuromorphic Computing in Non-Volatile Random Access Memory
Authors: Ou Qiao-Feng , Xiong Bang-Shu , Yu Lei , Wen Jing , Wang Lei , Tong Yi
Published: August 2020

Recent progress in the development of artificial intelligence technologies, aided by deep learning algorithms, has led to an unprecedented revolution in neuromorphic circuits, bringing us ever closer to brain-like computers. However, the vast majority of advanced algorithms still have to run on conventional computers. Thus, their capacities are limited by what is known as the von-Neumann bottleneck, where the central processing unit for data computation and the main memory for data storage are separated. Emerging forms of non-volatile random access memory, such as ferroelectric random access memory, phase-change random access memory, magnetic random access memory, and resistive random access memory, are widely considered to offer the best prospect of circumventing the von-Neumann bottleneck. This is due to their ability to merge storage and computational operations, such as Boolean logic. This paper reviews the most common kinds of non-volatile random access memory and their physical principles, together with their relative pros and cons when compared with conventional CMOS-based circuits (Complementary Metal Oxide Semiconductor). Their potential application to Boolean logic computation is then considered in terms of their working mechanism, circuit design and performance metrics. The paper concludes by envisaging the prospects offered by non-volatile devices for future brain-inspired and neuromorphic computation.
Concrete Mix Design for Completely Recycled Fine Aggregate by Modified Packing Density Method
Authors: Yang Yibo , Chen Baixi , Su Yan , Chen Qianpu , Li Zhiji , Guo Wenying , Wang Hengchang
Published: August 2020

The undesirable properties of conventional recycled fine aggregate (RFA) often limit its application in the construction industry. To overcome this challenge, a method for preparing completely recycled fine aggregate (CRFA), which crushes all concrete waste only into fine aggregate, was proposed. The obtained CRFA had high apparent density, and its water absorption was lower than that of the conventional RFA. To take advantage of the CRFA, this paper introduced the modified packing density method for the CRFA concrete mix design. The modified packing density method took account of the powder with a particle size of smaller than 75 μm in the CRFA and balanced both the void ratio and the specific surface area of the aggregate system. Concrete (grade C55) was prepared using the CRFA to validate the feasibility of the proposed method. The unit price of the prepared CRFA concrete was around 12.7% lower than that of the natural aggregate concrete. Additionally, the proposed procedure for the concrete mixture design could recycle all concrete waste into the new concrete and replace all the natural fine aggregate in the concrete mixture.
Possibility of Utilization of Gate Geometry to Modify the Mechanical and Structural Properties of Castings on the Al-Si Basis
Authors: Majernik Jan , Gaspar Stefan , Kmec Jan , Karkova Monika , Mascenik Jozef
Published: August 2020

The mechanical properties of die castings correlate with the inner structure of a casting, distribution of the eutectic phases, and with the content and distribution of porous cavities in the casting volume. This submitted paper deals with the issue of the possibility to influence the basic parameters affecting the quality of castings through structural modifications of the gating system. The structural parameter under assessment is the gate height. In the case of the diverse height of the gate, five sets of castings were produced. The individual sets of castings were subjected to examination of selected mechanical properties, i.e., of permanent deformation and surface hardness. At the same time, the individual sets of castings were subjected to metallographic examination of the eutectic structure of the casting. It was proved that the gate height influences the aforementioned properties of the castings and significantly affects the ratio of the eutectic phases in the volume of the casting. The conclusion describes the mutual correlation between the gate structure, the mechanical properties of the casting, and its structural composition.
Feasibility of Using a Prosthetic-Based Impression Template to Improve the Trueness and Precision of a Complete Arch Digital Impression on Four and Six Implants: An In Vitro Study
Authors: Tallarico Marco , Lumbau Aurea , Scrascia Roberto , Demelas Gianluca , Sanseverino Franco , Amarena Rocco , Meloni Silvio
Published: August 2020

Background: Intraoral scanners (IOSs) in implantology represent a viable approach for single teeth or partial arches. However, when used for complete edentulous arches or long-span edentulous areas, it has been demonstrated that there is a need for improvement of IOS-related techniques. Therefore, the aim of this in vitro study was to assess the trueness and precision of a complete arch digital impression on four and six implants taken with or without a customized, prosthetic-based impression template. Materials and Methods: Two experimental models were prepared, representative of a complete edentulous mandible restored with four and six implants with built-in scan abutments. Models were scanned with (test group, TG) or without (control group, CG) the prosthetic-based impression template. Eight scans were taken for each model. The time needed to take impressions, error, trueness, and precision were evaluated. A statistical analysis was performed. Results: In the case of four implants, the time needed for the impression was 128.7 ± 55.3 s in the TG and 81.0 ± 23.5 s in the CG (p = 0.0416). With six scan abutments, the time was 197.5 ± 26.8 and 110.6 ± 25.2 s in the TG and CG, respectively (p = 0.0000). In the TG, no errors were experienced, while in the CG, 13 impressions were retaken due to incorrect stitching processes. In the four-implant impression, the mean angle deviation was 0.252 ± 0.068° (95% CI 0.021–0.115°) in the CG and 0.134 ± 0.053° (95% CI 0.016–0.090°) in the TG. The difference was statistically significant (p = 0.002). In the six-implant impression, the mean angle deviation was 0.373 ± 0.117° (95% CI 0.036–0.198°) in the CG and 0.100 ± 0.029° (95% CI 0.009–0.049°) in the TG (p = 0.000). In the TG, there were no statistically significant differences in the mean angle deviation within the group (p > 0.05), but there were in the CG. A colorimetric analysis showed higher deviations from the original model for the six-implant impression without a prosthetic template. Conclusions: Although all of the impressions exhibited deviation from the original model in the range of clinical acceptability, the prosthetic-based impression template significantly improved the trueness and precision of complete edentulous arches rehabilitated with four or six implants, making the complete arch digital impression more predictable.
Additive Re-Manufacturing of Mechanically Recycled End-of-Life Glass Fiber-Reinforced Polymers for Value-Added Circular Design
Authors: Romani Alessia , Mantelli Andrea , Suriano Raffaella , Levi Marinella , Turri Stefano
Published: August 2020

Despite the large use of composites for industrial applications, their end-of-life management is still an open issue for manufacturing, especially in the wind energy sector. Additive manufacturing technology has been emerging as a solution, enhancing circular economy models, and using recycled composites for glass fiber-reinforced polymers is spreading as a new additive manufacturing trend. Nevertheless, their mechanical properties are still not comparable to pristine materials. The purpose of this paper is to examine the additive re-manufacturing of end-of-life glass fiber composites with mechanical performances that are comparable to virgin glass fiber-reinforced materials. Through a systematic characterization of the recyclate, requirements of the filler for the liquid deposition modeling process were identified. Printability and material surface quality of different formulations were analyzed using a low-cost modified 3D printer. Two hypothetical design concepts were also manufactured to validate the field of application. Furthermore, an understanding of the mechanical behavior was accomplished by means of tensile tests, and the results were compared with a benchmark formulation with virgin glass fibers. Mechanically recycled glass fibers show the capability to substitute pristine fillers, unlocking their use for new fields of application.
Chitosan-Hydrogel Polymeric Scaffold Acts as an Independent Primary Inducer of Osteogenic Differentiation in Human Mesenchymal Stromal Cells
Authors: Bernardi Simona , Re Federica , Bosio Katia , Dey Kamol , Almici Camillo , Malagola Michele , Guizzi Pierangelo , Sartore Luciana , Russo Domenico
Published: August 2020

Regenerative medicine aims to restore damaged tissues and mainly takes advantage of human mesenchymal stromal cells (hMSCs), either alone or combined with three-dimensional scaffolds. The scaffold is generally considered a support, and its contribution to hMSC proliferation and differentiation is unknown or poorly investigated. The aim of this study was to evaluate the capability of an innovative three-dimensional gelatin–chitosan hybrid hydrogel scaffold (HC) to activate the osteogenic differentiation process in hMSCs. We seeded hMSCs from adipose tissue (AT-hMSCs) and bone marrow (BM-hMSCs) in highly performing HC of varying chitosan content in the presence of growing medium (GM) or osteogenic medium (OM) combined with Fetal Bovine Serum (FBS) or human platelet lysate (hPL). We primarily evaluated the viability and the proliferation of AT-hMSCs and BM-hMSCs under different conditions. Then, in order to analyse the activation of osteogenic differentiation, the osteopontin (OPN) transcript was absolutely quantified at day 21 by digital PCR. OPN was expressed under all conditions, in both BM-hMSCs and AT-hMSCs. Cells seeded in HC cultured with OM+hPL presented the highest OPN transcript levels, as expected. Interestingly, both BM-hMSCs and AT-hMSCs cultured with GM+FBS expressed OPN. In particular, BM-hMSCs cultured with GM+FBS expressed more OPN than those cultured with GM+hPL and OM+FBS; AT-hMSCs cultured with GM+FBS presented a lower expression of OPN when compared with those cultured with GM+hPL, but no significant difference was detected when compared with AT-hMSCs cultured with OM+FBS. No OPN expression was detected in negative controls. These results show the capability of HC to primarily and independently activate osteogenic differentiation pathways in hMCSs. Therefore, these scaffolds may be considered no more as a simple support, rather than active players in the differentiative and regenerative process.
Analysis of a Mathematical Model. Opportunities for the Training of Food Engineering Students
Authors: Sepúlveda Edwin , González-Gómez Difariney , Villa-Ochoa Jhony Alexander
Published: August 2020

International research has revealed different roles of mathematics in the practices of engineers and some implications of mathematics teaching for engineering students. Modeling and mathematical models have proven to be valuable tools for their professional work and for their teaching process. This study identifies opportunities offered by a process of analysis of a mathematical model in the training of engineers. For this analysis, an interpretation of mathematical models as an object–user–representation triad was used; mathematical models were also considered a pedagogical approach to mathematics teaching. Based on this approach, a qualitative study was developed. A teaching experiment was designed, in which, through a set of tasks, the analysis of a model describing the percentage of moisture removed in a radial airflow food dryer is considered. Results show that students evidenced a comprehension of the model function as a covariation relationship and implemented strategies for understanding it through the graphs in the model. The situated character of students’ reasoning and their experience with professional practices of engineers are also highlighted.
Decentralized Adaptive Tracking of Interconnected Nonlinear Systems by Corrupted Output Feedback
Authors: Jeong Dong Min , Yoo Sung Jin
Published: August 2020

A decentralized adaptive resilient output-feedback stabilization strategy is presented for a class of uncertain interconnected nonlinear systems with unknown time-varying measurement sensitivities. In the concerned problem, the main difficulty is to achieve the decentralization of interconnected output nonlinearities unmatched to the control input by using only local output information corrupted by measurement sensitivity, namely the exact output information cannot be used to design the decentralized output-feedback control scheme. Thus, a decentralized output-feedback stabilizer design using only the corrupted output of each subsystem is developed where the adaptive control technique is employed to compensate for the effects of unknown measurement sensitivities. The stability of the resulting decentralized control scheme is analyzed based on the Lyapunov stability theorem.
Tracking Control Strategy Using Filter-Based Approximation for the Unknown Control Direction Problem of Uncertain Pure-Feedback Nonlinear Systems
Authors: Choi Yun Ho , Yoo Sung Jin
Published: August 2020

A filter-based recursive tracker design approach is presented for the problem of unknown control directions of pure-feedback systems with completely unknown non-affine nonlinearities. In the controller design procedure, the first-order filters for error surfaces, a control input, and state variables are employed to design nonadaptive virtual and actual control laws independent of adaptive function approximators. In addition, for the unknown control direction problem, the filtering signals are incorporated with Nussbaum functions. Different from existing adaptive approximation-based control schemes in the presence of unknown control directions, the proposed approach does not require any adaptive technique regardless of completely unknown nonlinear functions. Therefore, a simplified tracking structure can be constructed. Using the Lyapunov stability analysis, it is shown that the tracking error is reduced within an adjustable neighborhood of the origin while ensuring all the closed-loop signals are bounded.
Application of Improved Best Worst Method (BWM) in Real-World Problems
Authors: Pamučar Dragan , Ecer Fatih , Cirovic Goran , Arlasheedi Melfi A.
Published: August 2020

The Best Worst Method (BWM) represents a powerful tool for multi-criteria decision-making and defining criteria weight coefficients. However, while solving real-world problems, there are specific multi-criteria problems where several criteria exert the same influence on decision-making. In such situations, the traditional postulates of the BWM imply the defining of one best criterion and one worst criterion from within a set of observed criteria. In this paper, an improvement of the traditional BWM that eliminates this problem is presented. The improved BWM (BWM-I) offers the possibility for decision-makers to express their preferences even in cases where there is more than one best and worst criterion. The development enables the following: (1) the BWM-I enables us to express experts’ preferences irrespective of the number of the best/worst criteria in a set of evaluation criteria; (2) the application of the BWM-I reduces the possibility of making a mistake while comparing pairs of criteria, which increases the reliability of the results; and (3) the BWM-I is characterized by its flexibility, which is expressed through the possibility of the realistic processing of experts’ preferences irrespective of the number of the criteria that have the same significance and the possibility of the transformation of the BWM-I into the traditional BWM (should there be a unique best/worst criterion). To present the applicability of the BWM-I, it was applied to defining the weight coefficients of the criteria in the field of renewable energy and their ranking.
Modeling of Fluid Flow and Residence-Time Distribution in a Five-Strand Tundish
Authors: Sheng Dong-Yuan , Yue Qiang
Published: August 2020

Quantified residence-time distribution (RTD) provides a numerical characterization of mixing in the continuous casting tundish-thus allowing the engineer to better understand the metallurgical performance of the reactor. This study describes a computational fluid dynamic (CFD) modeling study for analyzing the flow pattern and the residence-time distribution in a five-strand tundish. Two passive scalar-transport equations were applied to separately calculate the E-curve and F-curve in the tundish. The numeric modeling result were compared to water-modeling results to validate the mathematical model. The volume fraction of different flow regions (plug, mixed and dead) and the intermixing time during the ladle changeover were calculated to study the effects of the flow control device (FCD) on the tundish performance. From the results of CFD calculations, it can be stated that a combination of the U-baffle with deflector holes and the turbulence inhibitor had three major effects on the flow characteristics in the tundish: (i) to reduce the extent of the dead volume; (ii) to evenly distribute the liquid streams to each strand and (iii) to shorten the intermixing time during the ladle changeover operation.
A Novel Stick-Slip Nanopositioning Stage Integrated with a Flexure Hinge-Based Friction Force Adjusting Structure
Authors: Zhu Junhui , Pan Peng , Wang Yong , Gu Sen , Zhai Rongan , Pang Ming , Liu Xinyu , Ru Changhai
Published: August 2020

The piezoelectrically-actuated stick-slip nanopositioning stage (PASSNS) has been applied extensively, and many designs of PASSNSs have been developed. The friction force between the stick-slip surfaces plays a critical role in successful movement of the stage, which influences the load capacity, dynamic performance, and positioning accuracy of the PASSNS. Toward solving the influence problems of friction force, this paper presents a novel stick-slip nanopositioning stage where the flexure hinge-based friction force adjusting unit was employed. Numerical analysis was conducted to estimate the static performance of the stage, a dynamic model was established, and simulation analysis was performed to study the dynamic performance of the stage. Further, a prototype was manufactured and a series of experiments were carried out to test the performance of the stage. The results show that the maximum forward and backward movement speeds of the stage are 1 and 0.7 mm/s, respectively, and the minimum forward and backward step displacements are approximately 11 and 12 nm, respectively. Compared to the step displacement under no working load, the forward and backward step displacements only increase by 6% and 8% with a working load of 20 g, respectively. And the load capacity of the PASSNS in the vertical direction is about 72 g. The experimental results confirm the feasibility of the proposed stage, and high accuracy, high speed, and good robustness to varying loads were achieved. These results demonstrate the great potential of the developed stage in many nanopositioning applications.
Ultrasound-Attenuated Microorganisms Inoculated in Vegetable Beverages: Effect of Strains, Temperature, Ultrasound and Storage Conditions on the Performances of the Treatment
Authors: Campaniello Daniela , Corbo Maria Rosaria , Speranza Barbara , Sinigaglia Milena , Bevilacqua Antonio
Published: August 2020

Four microorganisms (Lactobacillus acidophilus LA5, Bifidobacterium animalis subsp. lactis DSM 10140 and Lactiplantibacillus plantarum c16 and c19) were attenuated through ultrasound (US) treatments (40% of power for 2, 4 and 6 min; and 60% for 2 min; pulses were set at 2 s) inoculated in rice–oats–almond–soy-based beverages and stored at 4 °C for eight days. All strains were able to survive throughout the storage independently by the food matrix. Concerning the effect on acidification, the results were analyzed through multifactorial analysis of variance (MANOVA) and the key-findings of this were: (i) The treatment with 40% of power for 6 min was the most efficient at delaying acidification; (ii) Lb. acidophilus LA5 showed the best capacity to delay acidification; (iii) in the soy-based beverage a lower acidification was found. In a second step, L. plantarum c16 and c19 were attenuated, inoculated in rice beverage, stored under a thermal abuse (for 4 and 24 h) and then at 4, 15 and 20 °C. The results showed that only when US were combined with refrigeration temperatures were they efficient at delaying acidification. Thus, a perspective for attenuation could be the optimization of the treatment to design an effective way to counteract acidification also under a thermal abuse.
Organic Carbon and Nitrogen Isoscapes of Reef Corals and Algal Symbionts: Relative Influences of Environmental Gradients and Heterotrophy
Authors: Fujii Takanori , Tanaka Yasuaki , Maki Koh , Saotome Nobue , Morimoto Naoko , Watanabe Atsushi , Miyajima Toshihiro
Published: August 2020

The elemental (C/N) and stable isotopic (δ13C, δ15N) compositions and compound-specific δ15N values of amino acids (δ15NAA) were evaluated for coral holobionts as diagnostic tools to detect spatiotemporal environmental heterogeneity and its effects on coral health. Hermatypic coral samples of eight species were collected at 12 reef sites with differing levels of pollution stress. The C/N ratios, δ13C values, and δ15N values of coral tissues and endosymbiotic algae were determined for 193 coral holobionts, and the amino acid composition and δ15NAA values of selected samples were analyzed. δ15N values were influenced most by pollution stress, while C/N ratios and δ13C values depended most strongly on species. The results imply that δ13C and δ15N values are useful indicators for distinguishing the ecological niches of sympatric coral species based on microhabitat preference and resource selectivity. Using δ15NAA values, the trophic level (TL) of the examined coral samples was estimated to be 0.71 to 1.53, i.e., purely autotrophic to partially heterotrophic. Significant portions of the variation in bulk δ15N and δ13C values could be explained by the influence of heterotrophy. The TL of symbionts covaried with that of their hosts, implying that amino acids acquired through host heterotrophy are translocated to symbionts. Dependence on heterotrophy was stronger at polluted sites, indicating that the ecological role of corals changes in response to eutrophication.
High-Resolution Magic Angle Spinning (HR-MAS) NMR-Based Fingerprints Determination in the Medicinal Plant Berberis laurina
Authors: Ali Sher , Badshah Gul , Da Ros Montes D’Oca Caroline , Ramos Campos Francinete , Nagata Noemi , Khan Ajmir , de Fátima Costa Santos Maria , Barison Andersson
Published: August 2020

Berberis laurina (Berberidaceae) is a well-known medicinal plant used in traditional medicine since ancient times; however, it is scarcely studied to a large-scale fingerprint. This work presents a broad-range fingerprints determination through high-resolution magical angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy, a well-established flexible analytical method and one of most powerful “omics” platforms. It had been intended to describe a large range of chemical compositions in all plant parts. Beyond that, HR-MAS NMR allowed the direct investigation of botanical material (leaves, stems, and roots) in their natural, unaltered states, preventing molecular changes. The study revealed 17 metabolites, including caffeic acid, and berberine, a remarkable alkaloid from the genus Berberis L. The metabolic pattern changes of the leaves in the course of time were found to be seasonally dependent, probably due to the variability of seasonal and environmental trends. This metabolites overview is of great importance in understanding plant (bio)chemistry and mediating plant survival and is influenceable by interacting environmental means. Moreover, the study will be helpful in medicinal purposes, health sciences, crop evaluations, and genetic and biotechnological research.
Evaluation of a Stable Isotope-Based Direct Quantification Method for Dicamba Analysis from Air and Water Using Single-Quadrupole LC–MS
Authors: Ghaste Manoj , Hayden Nicholas C. , Osterholt Matthew J. , Young Julie , Young Bryan , Widhalm Joshua R.
Published: August 2020

Dicamba is a moderately volatile herbicide used for post-emergent control of broadleaf weeds in corn, soybean, and a number of other crops. With increased use of dicamba due to the release of dicamba-resistant cotton and soybean varieties, growing controversy over the effects of spray drift and volatilization on non-target crops has increased the need for quantifying dicamba collected from water and air sampling. Therefore, this study was designed to evaluate stable isotope-based direct quantification of dicamba from air and water samples using single-quadrupole liquid chromatography–mass spectrometry (LC–MS). The sample preparation protocols developed in this study utilize a simple solid-phase extraction (SPE) protocol for water samples and a single-step concentration protocol for air samples. The LC–MS detection method achieves sensitive detection of dicamba based on selected ion monitoring (SIM) of precursor and fragment ions and relies on the use of an isotopically labeled internal standard (IS) (D3-dicamba), which allows for calculating recoveries and quantification using a relative response factor (RRF). Analyte recoveries of 106–128% from water and 88–124% from air were attained, with limits of detection (LODs) of 0.1 ng mL−1 and 1 ng mL−1, respectively. The LC–MS detection method does not require sample pretreatment such as ion-pairing or derivatization to achieve sensitivity. Moreover, this study reveals matrix effects associated with sorbent resin used in air sample collection and demonstrates how the use of an isotopically labeled IS with RRF-based analysis can account for ion suppression. The LC–MS method is easily transferrable and offers a robust alternative to methods relying on more expensive tandem LC–MS/MS-based options.
Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil
Authors: Francioso Antonio , Federico Rodolfo , Maggiore Anna , Fontana Mario , Boffi Alberto , D’Erme Maria , Mosca Luciana
Published: August 2020

Extra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protective and anti-cancer agents. These compounds cannot be easily obtained via chemical synthesis, and their isolation and purification from EVOO is cumbersome. Indeed, both processes involve the use of large volumes of organic solvents, hazardous reagents and several chromatographic steps. In this work we propose a novel optimized procedure for the green extraction, isolation and purification of HTyr, Tyr, oleacein and oleocanthal directly from EVOO, by using a Natural Deep Eutectic Solvent (NaDES) as an extracting phase, coupled with preparative high-performance liquid chromatography. This purification method allows the total recovery of the four components as single pure compounds directly from EVOO, in a rapid, economic and ecologically sustainable way, which utilizes biocompatible reagents and strongly limits the use or generation of hazardous substances.
Titanium Dioxide Grafted on Graphene Oxide: Hybrid Nanofiller for Effective and Low-Cost Proton Exchange Membranes
Authors: Simari Cataldo , Lufrano Ernestino , Godbert Nicolas , Gournis Dimitrios , Coppola Luigi , Nicotera Isabella
Published: August 2020

A nanostructured hybrid material consisting of TiO2 nanoparticles grown and stabilized on graphene oxide (GO) platelets, was synthesized and tested as nanofiller in a polymeric matrix of sulfonated polysulfone (sPSU) for the preparation of new and low-cost nanocomposite electrolytes for proton exchange membrane fuel cell (PEMFC) applications. GO-TiO2 hybrid material combines the nanoscale structure, large interfacial area, and mechanical features of a 2D, layered material, and the hygroscopicity properties of ceramic oxides, able to maintain a suitable hydration of the membrane under harsh fuel cell operative conditions. GO-TiO2 was synthetized through a new, simple, one-pot hydrothermal procedure, while nanocomposite membranes were prepared by casting using different filler loadings. Both material and membranes were investigated by a combination of XRD, Raman, FTIR, thermo-mechanical analysis (TGA and Dynamic Mechanical Analysis) and SEM microscopy, while extensive studies on the proton transport properties were carried out by Electrochemical Impedance Spectroscopy (EIS) measurements and pulse field gradient (PFG) NMR spectroscopy. The addition of GO-TiO2 to the sPSU produced a highly stable network, with an increasing of the storage modulus three-fold higher than the filler-free sPSU membrane. Moreover, the composite membrane with 3 wt.% of filler content demonstrated very high water-retention capacity at high temperatures as well as a remarkable proton mobility, especially in very low relative humidity conditions, marking a step ahead of the state of the art in PEMs. This suggests that an architecture between polymer and filler was created with interconnected routes for an efficient proton transport.
Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
Authors: K. Anpalagan Karthiga , V. Karakkat Jimsheena , Truskewycz Adam , Saedi Ahmed Al , Joseph Paul , Apostolopoulos Vasso , Nurgali Kulmira , Cole Ivan , Cai Zibo , T. H. Lai Daniel
Published: August 2020

Biocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of concentrated mineral acids and other corrosive chemicals during the fabrication process curtails their biocompatibility and severely limits the utilization of the products in cell bio-imaging. In this study; a facile; fast; and cost-effective synthetic route is employed to fabricate CQDs from a natural organic resource; namely bread; where the use of any toxic chemicals is eliminated. Thus; the novel chemical-free technique facilitated the production of luminescent CQDs that were endowed with low cytotoxicity and; therefore; suitable candidates for bioimaging sensors. The above mentioned amorphous CQDs also exhibited fluorescence over 360–420 nm excitation wavelengths; and with a broad emission range of 360–600 nm. We have also shown that the CQDs were well internalized by muscle myoblasts (C2C12) and differentiated myotubes; the cell lines which have not been reported before.