bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role Of Psychological Capital Mediation On The Influence Of Auditor Experience On Audit Dysfunctional Behavior
Authors: Lismawati  , Nila Aprilla  , Riski Tri Septianita
Published: November 2024

This study aims to provide evidence of the effect of auditor experience on psychological capital, provide evidence of the effect of psychological capital on dysfunctional audit behaviour and provide evidence of the mediating effect of psychological capital on the effect of auditor experience on audit dysfunctional behaviour. This research includes quantitative descriptive research. Data collection in this study was carried out by distributing questionnaires to respondents online. The research respondents were internal auditors in Bengkulu Province. The number of auditors who became research respondents was 175 people, spread across 11 Inspectorate Offices in Bengkulu Province. The analysis method uses descriptive analysis and partial least square (PLS) analysis. The results showed that: (1) Auditor experience has a positive and significant influence on the psychological capital of auditors of the Inspectorate Office in Bengkulu Province; (2 Auditor psychological capital has a negative and significant influence on the dysfunctional behaviour of auditors of the Inspectorate Office in Bengkulu Province; and (3) Psychological capital has a mediating role in the effect of auditor experience on the dysfunctional behaviour of auditors of the Inspectorate Office in Bengkulu Province. Themediating role that occurs is partially mediation
Peningkatan Kualitas Kemasan Teh Bunga Telang Melalui Introduksi Teknologi Mesin Continuous Band Sealer
Authors: Khomah Isti , Kusnandar Kusnandar , Harisudin Mohamad , Riptanti Erlyna Wida , Setyowati Nuning , Qonita Raden Rara Aulia
Published: November 2024

This community service activities aim to introduce continuous band sealer machine technology to improve the quality of butterfly pea flower tea product packaging from Idako Sukses Mandiri MSMEs. The implementation of this community service uses participatory and mentoring methods. Activities are evaluated using before-after evaluation and descriptive analysis. The results of the service show that the Continous Band Sealer Machine has a positive impact on business sustainability. The Continous Band Sealer Machine can overcome the problems of MSMEs with limited production equipment in terms of packaging. Idako Sukses Mandiri MSMEs can use the machine well, and they will be more experienced and skilled. The positive impact can be seen from the quality of the packaging sealing; the sealing time becomes faster with a large amount, so the time used becomes efficient.
Pendampingan Optimalisasi Produksi pada Industri Rumah Tangga “Kerupuk Nasi Bersama” di Desa Pematang Baih Kabupaten Rokan Hulu
Authors: Daruwati Ika , Lesmana Andi , Anwar Saiful
Published: November 2024

This community service aims to increase the quality and quantity of rice cracker production in household households through the application of appropriate technology. The method of implementing this service used training and mentoring. The partners of this activity were members of the “Kerupuk Nasi Bersama” Home Industry in Pematang Baih Village, Rokan Hulu Regency, Riau Province. The results of this service showed that IRT partners had the ability to operate automatic cracker cutting equipment technology and dough mixer machines so that they could increase the quality and quantity of rice cracker production. This Appropriate Technology makes it easier for housewives to repair and increase product quality and quantity.
Pelatihan Pembuatan Pupuk Organik Cair Berbasis Bakteri Fotosintetik (PSB) bagi Kelompok Tani di Kabupaten Enrekang
Authors: Kining Ekajayanti , Ardat Muh. Achyar , Rusdin Rista Astari , Suharman Suharman , Assidiq Ilham , Badi Muslim , Basri Basri
Published: November 2024

This community service aims to transfer the technology of making Liquid Organic Fertilizer (POC) based on Photosynthetic Bacteria (PSB) for the Eran Batu Farmer Group in Pekalobean Village, Enrekang Regency. The method of implementing this community service uses Participatory Rural Appraisal (PRA). This program consists of several stages: (1) Socialization and training, (2) Practice of making POC, (3) Implementation of a demonstration plot for planting yard vegetables, (4) Counseling on product effectiveness, (5) Assistance in product marketing, and (6) Monitoring and evaluation of the program. The results of the community service showed a significant increase in participant knowledge and skills. Pre-test and post-test analysis showed an increase in understanding of 42.59%, with an average score increasing from 68.73 to 98.00. The practice of making POC achieved a success rate of 90%, with 95% of participants reporting a significant increase in independent production capabilities. The implementation of the demonstration plot showed an increase in crop productivity of 22% compared to conventional methods, accompanied by improvements in soil health and plant resistance to pests and diseases. This community service program has succeeded in transferring PSB-based POC technology and encouraging sustainable agricultural practices among farmers of the Eran Batu Farmers Group.
Analisis Kelimpahan Gastropoda Di Perairan Pantai Nguling Di Kecamatan Nguling Kabupaten Pasuruan
Authors: Lathifah Iffah , Laili Saimul , Syauqi Ahmad
Published: November 2024

Pantai merupakan satu di antara beberapa ekosistem yang telah lama dimanfaatkan oleh manusia untuk berbagai aktivitas dalam menunjang kehidupan. Fungsi pantai semakin beragam seiring dengan kemajuan peradaban dan kebudayaan manusia yang mengakibatkan fungsi dari pantai tidak sesuia dengan peruntukan unuk mendukung kehidupan organisme akuatik dan kebutuhan masyarakat sekitar pantai. Hal ini yang menyebabkan penurunan kualitas pantai disebabkan oleh masuknya berbagai buangan limbah dari berbagai aktivitas manusia sehingga menyebabkan terjadi perubahan kualitas fisika dan kimia pantai tersebut. Salah satu biota laut yang diduga secara langsung akan langsung terpengaruh keberadaannya akibat penurunan kualitas air dan sedimen di lingkungan pantai adalah gastropoda. Metode yang digunakan pada penelitian ini adalah metode deskriptif kualitatif, metode deskriptif dengan pengujian langsung di lakukan di lapangan dan di laboratorium. Hasil pada penelitian yang telah dilakukan diketahui bahwa kondisi perairan pantai berbeda antara tiap stasiun antar tercemar sedang dan tercemar ringan. Indeks Diversitas Gastropoda yang di teliti pada ketiga stasiun menunjuka perbedaan 1-2 (kategori tercemar sedang) dan pada stasiun 2 (kategori tercemar sangat ringan) dan stasiun 3 bernilai antar 2-3 (tercemar ringan).
Antibakteri Keracunan Bahan Pangan Pada Formulasi Sediaan Sabun Mandi Kombucha Bunga Telang Sebagai Produk Bioteknologi Farmasi Dari 3 Lokasi Budidaya
Authors: Fadhillah Maya , Rezaldi Firman , Yenny Ratna Fitry , Maritha Vevi , Ayuwardani Novi , Suminar Erni
Published: November 2024

Produk bioteknologi farmasi dalam bentuk formulasi dan sediaan sabun mandi cair kombucha bunga telang dapat dimanfaatkan sebagai salah satu sediaan farmasi atau kosmetik yang bersifat antibakteri penyebab keracunan bahan pangan dan sebelumnya telah teruji pada formulasi dan sediaan sabun cuci tangan berbahan aktif kombucha bunga telang, sehingga pada penelitian ini penulis tertarik untuk menggunakan 3 lokasi budidaya tanaman bunga telang. Penelitian ini bertujuan untuk membuat formulasi dan sediaan sabun mandi cair berbahan aktif kombucha bunga telang yang diperoleh dari tiga lokasi budidaya yaitu kampung pekuncen kota cilegon, kampung cinangka anyer kabupaten serang, dan desa banjarwangi kabupaten pandeglang banten. Penelitian ini bersifat eksperimental laboratorium yaitu dengan cara membuat basis sabun mandi cair tanpa zat aktif, membuat basis sabun mandi cair dengan bahan aktif kombucha bunga telang pada konsentrasi 20%, 30%, dan 40% yang diperoleh dari 3 lokasi budidaya, dan menyediakan sabun mandi pasaran sebagai kontrol positif. Metode dalam pengujian sediaan farmasi (kosmetik/sabun mandi cair) melalui difusi cakram. Data mengenai rata-rata diameter zona hambat dianalisis melalui ANOVA satu jalur jika terdapat perbedaan secara signifikan dilanjut melalui analisis pos hoc Hasil penelitian ini telah terbukti bahwa masing-masing bunga telang yang dibudidayakan dari 3 lokasi dan juga dibuat sebagai formulasi dan sediaan sabun mandi cair kombucha berkolerasi secara positif dalam menghambat keemepat pertumbuhan bakteri uji. Berdasarkan analisis ANOVA satu jalur dengan masing-masing nilai P<0,05, maka dapat dilakukan uji lanjut berupa analisis pos hoc dimana formulasi dan sediaan sabun mandi cair berbahan aktif kombucha bunga telang dari 3 lokasi budidaya pada konsentrasi 40% berbeda nyata dengan konsentrasi 20% dan 30% sebagai antibakteri penyebab keracunan bahan pangan baik yang dihasilkan dari Lokasi budidaya kampung pekucen, kota cilegon banten, kampung cinangka kabupaten serang, banten, dan desa banjarwangi, kabupaten pandeglang, banten.
Identifikasi Mikroorganisme Rendaman Cairan Pembersih Lensa Kontak
Authors: Larasati Marsella , Mahtuti Erni Yohani , Rahmawati Previta Zeizar
Published: November 2024

Satu diantara aspek risiko utama adanya kontaminasi mikroba pada lensa kontak yakni rendahnya kebersihan dan kepatuhan terhadap perawatan lensa yang apabila terkontaminasi akan menyebabkan infeksi pada mata. Tujuan penelitian adalah mengidentifikasi mikroorganisme pada cairan rendaman pembersih lensa kontak. Jenis penelitian berikut ialah studi deskriptif dengan analitik, menggunakan random sampling. Sampel berupa rendaman cairan lensa kontak setelah pakai sebanyak 10 sampel. Tahapan penelitian meliputi pengambilan sampel rendaman cairan pembersih lensa kontak, pembuatan isolasi koloni bakteri dengan media MSA dan MCA, pembuatan biakan murni pada media supaya miring dan observasi mikroskopis koloni bakteri pada media MCA dan MSA, di lanjutkan uji biokimia serta uji enzimatik. Hasil penelitian menunjukkan koloni bakteri adalah bentuk batang, gram-negatif, berwarna merah, menyebar dan bakteri gram positif, bentuk bulat atau coccus, berkelompok, berwarna ungu. Pengujian biokimia memaparkan laktosa (-), glukosa (+),maltosa (-), mannitol (-), H2S (+), sukrosa (-), Mr (+), indol (+),citrate (+), Vp (-), mengarah pada spesies Pseudomonas aeruginosa. Hasil uji enzimatik meliputi katalase didapatkan hasil positif dengan adanya gelembung udara yang menunjukkan bakteri tersebut adalah Staphylococcus aureus. Saran selanjutnya mengidentifikasi bakteri gram positif dengan uji koagulase agar lebih spesifik menentukan diferensiasi bakteri Staphylococcus aureus dari spesies Staphylococcus lainnya.
Aktivitas Antibakteri Staphylococcus aureus Sediaan Gel dengan Berbagai Jenis Gelling Agent
Authors: Widyaningrum Ike , Sukmana Achdan , Azmi Nur Iza Ulul
Published: November 2024

Sediaan gel adalah jenis sediaan semipadat yang memiliki sifat bening, dingin, serta mudah diaplikasikan terutama untuk sediaan topikal. Bahan penyusun yang dibutuhkan untuk membuat sediaan tersebut adalah basis gel atau gelling agent. Berdasarkan asalnya gelling agent dibagi menjadi beberapa golongan yakni, sintetik, semisintetik, dan alami. Maisng-masing golongan terdapat contoh bahan yang kerap digunakan untuk membuat sediaan gel, yakni karbopol dari golongan sintetik, HPMC dari golongan semisintetaik, dan Xanthan gum dari golongan alami. Pada penelitian ini akan dilakukan pengamatan pengaruh dari berbagai jenis gelling agent terhadap aktifitas antibekateri. Bahan aktif yang digunakan sebagai model penelitian ini adalah asam salisilat. Asam salisilat secara teori disebutkan bahwa memiliki aktifitas antibakteri Bakteri yang digunakan pada penelitian ini adalah Staphylococcus aureus. Diketahui bahwa bakteri tersebut merupakan flora normal yang apabila pada jumlah berlebih akan menyebabkan infeksi pada kulit seperti jerawat, bisul, selulits dan lain lain.
DESIGNING 'KIDDOCARE' APPLICATION FOR PEDIATRIC NURSING PRACTICE WITH USER CENTERED DESIGN (UCD)
Authors: Yanti Rifa , Aldo Dasril , Putra Nursaka , Adi Dading Qolbu , Ilmi Miftahul
Published: November 2024

This research aims to develop KiddoCare, a mobile-based pediatric nursing application designed using the User Centered Design (UCD) method. The methodology used in this study included interviews, questionnaire distribution, and documentation with the aim of filling the gap of tools and applications that specifically meet the unique needs in pediatric care. Interviews with health workers showed that one of the biggest challenges was the lack of efficient communication between nurses and parents, as well as difficult access to pediatric health information. Therefore, this app is designed to improve communication between nurses and pediatric patients and facilitate access to important health information. The UCD process includes understanding the user context, determining user needs, designing and producing solutions, and evaluating those needs. From the initial survey of 74 respondents, 50.56% agreed and 46.02% strongly agreed with the need for easier access to pediatric health information. A total of 74 respondents evaluated the app and gave positive feedback; 47.01% 'agreed', 41.17% 'strongly agreed' with the functionality of the app, and 11.82% 'moderately'. No respondents expressed 'disagree' or 'strongly disagree'. In conclusion, KiddoCare was rated as an effective and appropriate pediatric nursing tool, supporting flexible care that is adaptive to each child's individual needs.
Shading from topographical complexity mitigates bleaching severity of Pocillopora spp. during thermal stress
Authors: Liesegang MB , Edwards CB , Pedersen NE , Petrovic V , Sandin SA
Published: November 2024

Thermally induced bleaching susceptibility of coral varies among species, regional environmental conditions, and local spatial patterns on a reef. As bleaching events become more frequent and severe, understanding the factors that increase resiliency against thermal stress is critical. Here, we used mapping products from large-area imagery collected at the remote atoll of Palmyra in the central Pacific during the 2015 marine heatwave to investigate the role of topographically produced shade in modifying bleaching severity. Change in planar area over 2 yr, bleaching severity, and estimated amount of shade provided by neighboring reef structures were measured for the common coral genus Pocillopora. Bleaching severity was correlated with the estimated irradiance, as colonies in more exposed areas (and thus receiving more daily sunlight) experienced more severe bleaching than corals in more shaded areas. Structural complexity and variability in reef topography provide regions of shade where the compound impact of temperature and irradiance is alleviated, resulting in less severe bleaching outcomes for populations of this cosmopolitan coral taxon. While some studies have considered artificially shading reefs during warm water events, we highlight the importance of existing structural complexity in providing shade, ultimately contributing to the mitigation of thermal impacts in the face of a changing climate.
PERANCANGAN SISTEM PENUNJANG KEPUTUSAN PENENTUAN JURUSAN DENGAN METODE SMART BERBASIS WEBSITE
Authors: Fidianto Muhammad Risqi
Published: November 2024

Salah satu kegiatan yang harus dilakukan oleh Mahasiswa yaitu pemilihan jurusan. Pemilihan jurusan merupakan salah satu proses penempatan atau penyaluran dalam pemilihan program pengajaran pada Mahasiswa/i di jenjang Penguruan tinggi.pada proses Jurusan pada tingkat pengguruan tinggi membuat calon mahasiswa baru sulit menentukan pilihan yang sesuai dengan kemampuan yang dimiliki.pada proses pendaftaran mahasiswa baru pada universitas narotama sendiri masih menggunakan metode manul berupa form pendaftaran. Maka dari itu terciptalah penelitian ini yang bertujuan untuk membuat sebuah aplikasi sistem penunjang keputusan dengan menggunakan metode Simple Multi Attribute Rating Technique (SMART) dalam pemilihan jurusan mahasiswa baru.Sistem Pendukung Keputusan yang dirancang mampu memberikan keputusan untuk penentuan jurusan secara cepat dan tepat melalui alternatif dan kriteria yang telah ditentukan sehingga dapat membantu siswa dalam memilih jurusan [1] sehingga hasilnya lebih optimal. Diharapkan penelitian ini dapat memberikan dampak terhadap sistem rekomendasi jurusan agar bisa menjadi lebih optimal dan efisien
Model Naïve Bayes dan Decision Tree untuk Prediksi Penerimaan Spesifikasi Mobil
Authors: Whidhiasih Retno Nugroho
Published: November 2024

Mobil merupakan kebutuhan setiap orang sebagai alat transportasi. Seseorang yang telah berkeluarga akan mempunyai kebutuhan yang lebih terhadap sebuah mobil, terlebih jika mempunyai keluarga besar. Jika mempunyai keluarga besar, memilih sebuah mobil yang tepat untuk keluarga sangatlah penting, dan banyak hal yang harus diperhatikan dalam memilih sebuah mobil. Ketepatan memilih mobil untuk suatu keluarga besar akan berpengaruh terhadap pemenuhan semua kebutuhan transportasi dari setiap anggota keluarga. Dalam penelitian ini dilakukan evaluasi terhadap variabel-variabel yang dipertimbangkan dalam memilih sebuah mobil, yaitu berdasarkan harga beli, biaya maintenance, faktor kenyamanan yang meliputi jumlah pintu, kapasitas orang yang bisa diangkut, ukuran bagasi dan perkiraan keamanan mobil dari data car evaluation. Evaluasi penerimaan dilakukan dengan melakukan prediksi penerimaan menggunakan algoritma naïve bayes dan decision tree classifier. Output dari evaluasi ini adalah penerimaan terhadap variabel-variabel yang dipertimbangkan dalam memilih sebuah mobil tersebut, yang dikategorikan kedalam empat kelas kategori, yaitu unacc, acc, good dan vgood. Kedua algoritma classifier tersebut menghasilkan kinerja yang berbeda, Naïve bayes menghasilkan akurasi 84,1% dan decision tree menghasilkan akurasi yang lebih tinggi yaitu 91,91%.
Aplikasi Daftar Kehadiran Mahasiswa Berbasis Kartu RFID Di Universitas Tanri Abeng
Authors: . Muhtar
Published: November 2024

Memasuki Era Pandemi dari Covid-19 membuat perguruan tinggi perlu mengikuti protokol kesehatan yang telah diberikan oleh pemerintah. Salah satu kegiatan yang dilakukan penyesuaian untuk menghindari kontak fisik adalah saat pengisian daftar hadir tanpa melakukan kontak fisik. Pada beberapa perguruan tinggi masih melakukan pengisian daftar hadir secara konvensional, yaitu dengan memanggil satu persatu mahasiswanya atau dengan menandatangani daftar kehadiran pada secarik kertas secara bergilir.  Selain menjaga jarak dengan tidak melakukan kontak fisik, tindakan pencegahan awal dari Covid-19 adalah pengecekan suhu tubuh. Suhu tubuh normal manusia terletak pada rentang 36,5 derajat Celsius hingga 37 derajat Celsius. Dalam penelitian ini penulis merancang dan menguji prototipe sistem daftar hadir menggunakan RFID dan sensor suhu Non-Contactless yang terhubung dengan web. RFID dan sensor suhu Non-Contactless digunakan untuk membatasi kontak fisik pada perguruan tinggi, sehingga penularan Covid-19 bisa dicegah.  Berdasarkan hasil penelitian ini, pembaca RFID dapat membaca kartu mahasiswa (RFID Tag) pada jarak optimal 3 cm, sedangkan pembacaan suhu pada jarak optimal 1-5 cm. Ketika RFID Tag didekatkan pada pembaca RFID, kecepatan waktu pembacaan yang diperlukan adalah 1-10 detik, tergantung dengan kecepatan server web dan koneksi internet pada Wifi yang terhubung pada NodeMCU.
IMPLEMENTASI CHATBOT DIAGNOSA PENYAKIT PADA KUCING DAN ANJING DENGAN METODE FORWARD CHAINING
Authors: Putra Bagas Krinasia
Published: November 2024

Currently, many people have made pets as pets and even treat them like humans, even considering pets as family. Among the various kinds of pets, cats and dogs are the most common pets. Cats and Dogs are animals that are popular among the public, their cute physical shape and adorable behavior is one of the reasons why many people like these pets. in the current technological developments that can provide information related to animal diseases, especially dogs and cats and how to provide first aid if the two animals are sick and provide a means for pet owners to get information quickly by implementing a chatbot based on Natural Language Processing and the application of an expert system which is a sub-field of artificial intelligence (Artificial Intelligence) with the forward chaining method. It is hoped that with the chatbot pet owners can find it easier to get answers quickly about their dog and cat diseases.
Keywords : Artificial Intelligence, Chatbot, Forward Chaining , NLP (Natural Language Processing).
Pengaruh Perancangan Gate Driver SiC Mosfet Terhadap Keandalan Sistem
Authors: Pradipta Doni
Published: November 2024

Pengendalian komponen elektronika daya diperlukannya driver pada gate sehingga diperlukannya perancangan gate driver bertujuan untuk memperkuat sinyal transmisi yang berasal dari control stage untuk memastikan switching yang sesuai dari power device, dalam perancangan harus memperhatikan rugi-rugi switching yang terjadi pada kondisi rise dan fall pada proses switching SiC Mosfet karena jika tidak dilakukan pemasangan gate resistance pada SiC Mosfet pada saat menjelang kondisi steady state terdapat lonjakan tegangan serta ripple yang cukup tinggi sehingga rugi-rugi daya semakin tinggi, oleh karena itu dilakukanny perlunya dilakukan penyesuaian terhadap nilai resistor yang dipasang pada gate SiC Mosfet baik dalam sisi on maupun off karena semakin kecil rugi-rugi switching maka perancangan gate driver semakin baik. Dalam penelitian ini digunakan model perancangan gate driver menggunakan analisis beban resistif dan beban induktif dengan rangkaian simulasi pabrik ROHM yang tertera dalam datasheet SiC MOSFET tipe sct2160ke yang disimulasikan menggunakan software TINA-TI. Dalam paper ini akan ditampilan hasil simulasi dan akan dilakukan analisis pengaruh gate resistance terhadap delay time on, delay time off, disipasi daya, dan switching energy measurement.
‘We/One devastate(s) the world’: Of Deserts, Chaos & Khōra
Authors: Martell James
Published: November 2024

If the word ‘devastated’ feels most appropriate when thinking of the passing of a beloved one, it also undergirds, through the waste of its desert, two essential notions of Western philosophy and literature: chaos and khōra. Looking at our world twenty years after Derrida’s passing, and at how for more than five decades he thought and wrote on the notion of world, this essay examines the radicality of Derrida’s deconstruction for our past and future conceptions of a world, of ourselves, and of each and any threshold between these terms.
Ceterum censemus Carthaginem Nouam esse legendam: Roman Poetry as Cultural History
Authors: González Berdús Victoria , Cenati Chiara , Kruschwitz Peter
Published: November 2024

This paper updates the carmina Latina epigraphica corpus of Carthago Nova (Hispania Citerior). The beginning of the arrival of families of Italic origin around the middle of the first century B.C. seems to be of crucial importance, as it definitely influenced the development of both trade and mining in the region and verse epigraphy. The study of the onomastics of these texts allows us to trace their origins and social positions, as well as to provide key elements of the social and cultural history of Carthago Nova.
El imperfecto (HES. Th. 269): un reto interpretativo
Authors: García Zamora Patricia , Jiménez Delgado José Miguel
Published: November 2024

Este trabajo aborda la interpretación del imperfecto ".." en la Teogonía de Hesíodo (Th. 269), que ha planteado dos problemas principales: en primer lugar, su transitividad, si se trata de un ejemplo intransitivo del verbo ".." desconocido fuera de este pasaje: y en segundo lugar, su temporalidad, dado que muchos comentaristas lo consideran un antiguo resto del injuntivo de presente indoeuropeo.
Olympica Pindarica (II)
Authors: Liberman Gauthier
Published: November 2024

Remarques critiques, exégétiques et métriques sur les plus longues Olympiques de Pindare, incluant des propositions sur les autres poèmes de Pindare et sur le texte d’autres auteurs.
A RNA-seq-based study on differentially expressed genes related to the Inner Mongolia EV71 C33λ strain that invades Vero cells
Authors: Lai Xiong , Fan Yaochun , Li Hongxia , Tian Xiaoling , Yue Xuanzhi , Gao Sheng , Lei Xia , Qin Yuhan , Zhang Xiangnan , Mei Jun , Shi Yu , Li Huan , Wu Duoduo , Zhang Wenyu , Jia Xinrui , Fan Ruichun , Li Bin , Xing Wanjin
Published: November 2024

Enterovirus 71 (EV71) is a significant causative agent of hand, foot and mouth disease (HFMD). However, the precise mechanism by which EV71 infection leads to alterations in the immune response remain elusive. To address this knowledge gap, we conducted a study where we introduced the Inner Mongolia EV71 C33λ strain into Vero cells, derived from African green monkey kidney cells. Subsequently, we performed RNA sequencing (RNA-Seq) to investigate the changes in the transcriptome of these infected Vero cells. Our primary objective was to establish a foundational understanding that could inform future research on EV71-associated immune factors. In our study, we identified a total of 942 differentially expressed genes (DEGs) in Vero cells infected with Enterovirus 71 (EV71), with 568 gene exhibiting increased expression and 374 gene showing decreased expression. To elucidate the functional implications of these DEGs, we conducted a comprehensive functional enrichment analysis using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. This analysis revealed three genes that were significantly upregulated, which we subsequently validated using reverse transcription polymerase chain reaction technology (RT-qPCR). The RT-qPCR results were in strong agreement with our RNA-Seq data, confirming the reliability of our findings. This study represents the pioneering RNA-Seq analysis that delves into the cellular response of Vero cells to EV71 infection. Our results not only provide a foundational understanding of the molecular changes induced by EV71 but also offer crucial insights into the mechanisms by which EV71 modulates the host immune system. These insights are pivotal for future research endeavors aimed at developing effective therapeutic strategies against EV71 and related pathogens, as well as for understanding the broader implications of viral infections on host immunity.
Anaerobic membrane bioreactor (AnMBR) with external ultrafiltration membrane for the treatment of sugar beet vinasse
Authors: Bueno Beatriz Egerland , Muniz Brito André Luiz , Garcia Rea Victor. S. , Kurnianto Rifki Wahyu , Zaiat Marcelo , van Lier Jules. B.
Published: November 2024

Vinasse, a by-product of ethanol production, is generated at significant rates. While rich in nutrients such as calcium, magnesium, and potassium, its high solids, organic matter, acidity, and sulfate content pose challenges when disposed directly on soil, necessitating treatment. Anaerobic digestion is a viable solution, reducing organic pollution while recovering energy in the form of biogas, aligning with the biorefinery concept. Traditionally, sludge bed reactors and anaerobic contact reactors are utilized for vinasse processing, with sludge granulation being vital for treatment success. However, challenges such as sludge wash-out due to recalcitrant compounds, high solids concentration in the influent, low pH, salinity, and temperature hinder granule formation. Anaerobic membrane bioreactors (AnMBR) offer an alternative, simplifying treatment by integrating intensified pre- and post-treatment units. Due to complete sludge retention, AnMBRs achieve high COD removal efficiencies, yielding a suspended solids-free and largely disinfected effluent. Therefore, AnMBRs show promise for vinasse treatment, eliminating the need for sludge granulation and producing nutrient-rich effluent with minimal residual organics and suspended solids. In this study, an AnMBR equipped with an inside-out external crossflow ultrafiltration membrane was proposed for the treatment of vinasse. The AnMBR reached a COD removal efficiency of 95% ± 2.6% and produced 0.3 CH4 L. g COD removed-1 working at organic loading rates of 8 g COD. L-1 d-1 and membrane fluxes of 10 LMH. At organic loading rates of 10 g COD. L-1 d-1 and fluxes of 12 and 14 LMH, the COD removal efficiency decreased to 77% ± 11% and 73% ± 7.9%, respectively. The AnMBR technology represents an innovation for wastewater treatment, however, more research using the cross-flow configuration and different types of effluents is needed. Literature studies that address the treatment of sugar beet or sugarcane vinasse using AnMBR are still scarce. This study explored the potentials of AnMBR technology for vinasse treatment and contributes to the dissemination of this technology, opening new possibilities for vinasse processing.
Impacts of a warming climate on the non-breeding distribution of a classic differential migrant
Authors: O’Neal Dawn M. , Austin Suzanne H. , Ketterson Ellen D.
Published: November 2024

Many changes in species’ geographic distributions have been attributed to recent climate warming. One understudied phenomenon is the effect of climate change on differential migrants, species in which the sexes differ in distance migrated to and from the breeding range. We evaluated the impact of climate change on differential migration in the dark-eyed junco (Junco hyemalis) by assessing temporal and geographic changes in overall population abundance throughout the winter range over the past 60 years. We also compared the abundance of females in two wintering populations studied 15 years ago with historical abundances studied 45 years ago We observed a northward movement of the population as a whole and an increase in female abundance at higher latitudes that correlated with recent changes in winter climate. These findings suggest that climate change has reduced distance migrated in this species and increased the proportion of females wintering at higher latitudes, providing new insights into the impact of climate warming on migratory distance and winter distributions.
Research progress on FSH-FSHR signaling in the pathogenesis of non-reproductive diseases
Authors: Li Chenhe , Ling Yan , Kuang Haibin
Published: November 2024

Follicle-stimulating hormone (FSH), a glycoprotein hormone synthesized and secreted by the anterior pituitary gland, plays a critical role in reproductive development and regulation by binding to FSH receptor (FSHR). Beyond reproductive tissue, FSHRs have been identified in various non-reproductive tissues, indicating broader functions. FSH levels chronically rise during menopause and remain elevated in postmenopausal life. This increase in FSH level has been indicated to be associated with heightened risk of several non-reproductive diseases, including osteoporosis, hypercholesterolemia, type 2 diabetes mellitus, obesity, cardiovascular disease, Alzheimer’s disease, and certain cancers. In this review, we will examine the role of FSH-FSHR signaling in the pathogenesis of these non-reproductive diseases and explore therapeutic strategies targeting FSH-FSHR signaling pathways.
Association of cumulative non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio with the risk of cardiometabolic disease
Authors: Liu Luqing , Liu Shihe , Liao Yicheng , Zhang Xiaoxue , Wang Meixiao , Lin Liming , Zhu Chenrui , Wu Shouling , Wu Yuntao
Published: November 2024

Background

One measurement of non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) is considered to be associated with insulin resistance and cardiovascular disease (CVD) risk. This study aimed to exploring the association between cumulative non-high-density lipoprotein cholesterol/high-density lipoprotein cholesterol (Cum NHHR) exposure levels and the risk of cardiometabolic disease (CMD).

Methods

This prospective cohort study included 43,735 participants, who participated in three consecutive health examinations in 2006, 2008, 2010 and had no history of CMD or cancer. The participants were divided into quartiles bases on their cum NHHR. Multivariate Cox proportional hazards model was used to assess the association between cum NHHR and the risk of CMD. Additionally, the direct method of standardized ratios was employed to calculate the absolute risk of CMD attributable to cum NHHR.

Results

Over a median follow-up period of 10.92 years (IQR: 10.22–11.26 years), 7,388 participants were newly diagnosed with CMD. In the multivariate-adjusted model, participants in quartiles Q2, Q3 and Q4 showed a progressively increased relative risk of CMD compared to those in Q1, The fully adjusted hazard ratios (95% confidence intervals) for the risk of CMD in the Q2, Q3, and Q4 groups were 1.11 (1.04–1.20), 1.23 (1.14–1.32), and 1.29 (1.20–1.38), respectively, compared with the Q1 group. This association remained significant even after further adjustment for single measurements of NHHR. Moreover, cum NHHR was positively correlated with the absolute risk of CMD, cardiovascular diseases (CVD), and type 2 diabetes (T2DM).

Conclusions

Higher cum NHHR is significantly associated with an increased risk of CMD, independent of single-point NHHR level. Additionally, there are significant different strengths of correlations between cum NHHR and different diseases.
The diurnal pattern of cytokines, chemokines and growth factors in human saliva—a pilot study
Authors: Jasim Hajer , Ghafouri Bijar , Ernberg Malin
Published: November 2024

Background

Understanding of possible periodicity of cytokines, chemokines and growth factors is of great interest and provide valuable information for research into pathophysiological mechanism of inflammatory disease and chronic pain. Significant efforts have been made to identify different analytes in saliva. For precision and accuracy in measurement and interpretation of results, it is crucial to know the source of variability, especially the circadian variation for the analytes.

Objective

The study aimed to analyze circadian variation in 71 inflammatory markers in both unstimulated and stimulated saliva, as well as plasma, from a sample of healthy individuals.

Methods

Ten young adults participated. Unstimulated and stimulated whole saliva were collected at 3-h intervals between between 7:30 am and 7:30 pm. Blood samples were drawn in connection with the first and last saliva collection. All samples were analyzed using the U-PLEX 71-Plex assay.

Results

The analysis showed distinct clustering of the 71 inflammatory mediators between plasma and saliva. Furthermore, differences were also observed between stimulated and unstimulated saliva. The proteins were clustered into three groups that expressed different circadian rhythms. These clusters were stable over time in stimulated saliva but showed significant variability in unstimulated saliva (P < 0.05).

Conclusions

These results suggest that time of the day could influence the detection and interpretation of inflammatory markers and collecting saliva samples at consistent times across participants will help control for the natural fluctuations in salivary composition. The results encourage further exploration of salivary diagnostics, particularly in understanding circadian rhythms and localized immune responses.
Joint association of METS-IR and uric acid with stoke, mediated by C-reactive protein
Authors: Jiang Shan , Zhang Xinyi , Song Chengning , Wu Guangfu , Yang Aicheng
Published: November 2024

Objectives

To explore the dose-response relationship between the Metabolic Score of Insulin Resistance (METS-IR), uric acid (UA) and the risk of stroke incidence, the mediating role of C-reactive protein (CRP) in the above relationship, as well as the joint effect of METS-IR and UA on the risk of stroke incidence.

Methods

Participants from the CHARLS study were included in this cohort study. Logistic regression models were used to estimate the odds ratios (ORs) and corresponding 95% confidence intervals (CIs) for the associations of METS-IR and UA with the risk of incident stroke. The dose-response relationships of METS-IR and UA with stroke risk were assessed by restricted cubic spline regression. The mediation models were employed to estimate the potential mediating effects of CRP on the associations of METS-IR and UA with stroke risk. Logistic regression analysis was carried out to analyse the association of stroke and MRTS-IR combined with UA.

Result

During a 9-year follow-up from 2011 to 2018, 570 incident cases of stroke were documented among 7,343 total participants. Per interquartile range increases in METS-IR and UA were associated with the increased risk of incident stroke, with the OR (95% CI) of 1.61 (1.44, 1.80) and 1.18 (1.05, 1.32) respectively. A dose-response function showed that METS-IR had a nonlinear relationship (P for nonlinear=0.047) and UA had a linear relationship (P for nonlinear=0.247) with the stroke risk. CRP had significant mediated effects on the associations of METS-IR and UA with stroke risk, and the proportion of mediation was 9.01% and 26.34% respectively (all P < 0.05). The results of joint effect showed that participants with high levels of METS-IR and UA had the highest increased risk of stroke compared to the participants with low levels of METS-IR and UA.

Conclusion

METS-IR and UA levels were positively associated with an increased risk of stroke onset, and CRP mediated these relationships. Improving insulin sensitivity and regulating CRP and uric acid levels may be important for preventing the risk of stroke occurrence.
Polystyrene microplastics exposition on human placental explants induces time-dependent cytotoxicity, oxidative stress and metabolic alterations
Authors: de Sousa Ashelley Kettyllem Alves , Pires Keyla Silva Nobre , Cavalcante Isadora Hart , Cavalcante Iasmin Cristina Lira , Santos Julia Domingues , Queiroz Maiara Ingrid Cavalcante , Leite Ana Catarina Rezende , Crispim Alessandre Carmo , da Rocha Junior Edmilson Rodrigues , Aquino Thiago Mendonça , Weingrill Rodrigo Barbano , Urschitz Johann , Ospina-Prieto Stephanie , Borbely Alexandre Urban
Published: November 2024

Introduction

Microplastics (MPs) are environmental pollutants that pose potential risks to living organisms. MPs have been shown to accumulate in human organs, including the placenta. In this study, we investigated the biochemical impact of 5 μm polystyrene microplastics (PS-MPs) on term placental chorionic villi explants, focusing on cytotoxicity, oxidative stress, metabolic changes, and the potential for MPs to cross the placental barrier.

Methods

Term placental chorionic explants were cultured for 24 hours with varying concentrations of PS-MPs, with MTT assays used to determine the appropriate concentration for further analysis. Cytotoxicity was assessed using the lactate dehydrogenase (LDH) release assay over a period of up to 72 hours. Reactive oxygen species formation and antioxidant activity were evaluated using biochemical assays. Metabolomic profiling was performed using proton nuclear magnetic resonance (1H NMR).

Results

Placental explants exposed to 100 μg/mL of PS-MPs showed a significant increase in cytotoxicity over time (p < 0.01). Levels of mitochondrial and total superoxide anion (p < 0.01 and p < 0.05, respectively) and hydrogen peroxide (p < 0.001) were significantly elevated. PS-MP exposure resulted in a reduction in total sulfhydryl content (p < 0.05) and the activities of antioxidant enzymes superoxide dismutase (p < 0.01) and catalase (p < 0.05), while glutathione peroxidase activity increased (p < 0.05), and the oxidized/reduced glutathione ratio decreased (p < 0.05). Markers of oxidative damage, such as malondialdehyde and carbonylated proteins, also increased significantly (p < 0.001 and p < 0.01, respectively), confirming oxidative stress. Metabolomic analysis revealed significant differences between control and PS-MP-exposed groups, with reduced levels of alanine, formate, glutaric acid, and maltotriose after PS-MP exposure.

Discussion

This study demonstrates that high concentrations of PS-MPs induce time-dependent cytotoxicity, oxidative stress, and alterations in the TCA cycle, as well as in folate, amino acid, and energy metabolism. These findings highlight the need for further research to clarify the full impact of MP contamination on pregnancy and its implications for future generations.
The therapeutic effects of curcumin on polycystic ovary syndrome by upregulating PPAR-γ expression and reducing oxidative stress in a rat model
Authors: Zhang Wei , Peng Cong , Xu Lei , Zhao Yutai , Huang Chaolin , Lu Ling
Published: November 2024

Objective

Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder that impacts 8-13% of women in their reproductive years. However, the drugs commonly used to treat PCOS are often prescribed off-label and may carry potential side effects. This study aimed to investigate the therapeutic effects of curcumin in a PCOS rat model.

Materials and methods

A PCOS rat model was established through daily subcutaneous injection of 60 mg/kg body weight of dehydroepiandrosterone (DHEA) for 21 days. The PCOS rats received a daily intragastric dose of 50 mg/kg body weight of curcumin for another 21 days. Ovarian morphological changes, estrous cycle changes, and hormone level changes were measured to evaluate the therapeutic effectiveness of curcumin in PCOS rats. Oxidative stress markers in the ovaries were analyzed to explore the mechanisms of curcumin in PCOS rats.

Results

This study demonstrated that curcumin alleviated insulin resistance and significantly reduced serum levels of estradiol (p = 0.02), luteinizing hormone (p = 0.009), testosterone (p = 0.003), and the LH/FSH ratio (p = 0.008) in PCOS rats. Curcumin also restored normal ovarian morphology and the estrous cycle in these rats. Furthermore, curcumin treatment significantly decreased levels of oxidative stress markers, including malondialdehyde (p = 0.004) and reactive oxygen species (p = 0.005), while increasing antioxidant levels such as superoxide dismutase (p = 0.04), glutathione peroxidase (p = 0.002), and glutathione (p = 0.02) in ovarian tissues. Additionally, curcumin significantly upregulated PPAR-γ in the ovarian tissues of PCOS rats.

Conclusion

This study demonstrates that curcumin effectively restores ovarian morphology, hormone levels, and estrous cycles in PCOS rats. These effects may be linked to its ability to reduce oxidative stress in ovaries via the upregulation of PPAR-γ. Curcumin shows promise as a potential drug for the treatment of PCOS.
Identification and spatial pattern analysis of abandoned farmland in Jiangxi Province of China based on GF-1 satellite image and object-oriented technology
Authors: Liang Yang , Liang Yiwen , Tu Xiaosong
Published: November 2024

Introduction

Industrialization, urbanization, wars, and conflicts have caused farmland abandonment and exacerbated food security issues, posing a major challenge to global food security. Therefore, it is of great significance to monitor the status of crop abandonment in major grain-producing areas. Most of previous studies using remote sensing technology to extract abandoned farmland have small scale and low accuracy, and there was lack of large-scale studies using GF-1 image. Particularly in the Jiangxi Province, as the main grain-producing area of China, the situation of farmland abandonment is still unknown.

Methods

In this paper, GF-1 WFV remote sensing images are used as the main data source. A binary decision tree process based on the object-oriented technology classification and vector similarity function change detection methods are adopted to extract abandoned farmland information in Jiangxi Province during 2020–2022 and to describe its spatial pattern.

Results

The results show that the overall accuracy of GF-1 remote sensing image extraction based on object-oriented technology is 93%, and the Kappa coefficient is 0.89. The abandoned farmland in Jiangxi Province covers an extensive area of 3.41 × 105 hm2, with an abandonment rate of 9.87%. Abandonment is greater in the north and less in the south, with a spatial distribution pattern characterized by sparse coverage in mountainous areas and aggregation in plains areas. Farmland abandonment is most severe in the areas surrounding the northern Poyang Lake Plain, and the degree of farmland abandonment varies significantly among various prefecture cities as well as among different counties. The highest rate of farmland abandonment in prefecture cities was 13.18% and the lowest was 7.13%. The highest rate of farmland abandonment in the county was 24.22%, and the lowest was 1.99%.

Discussion

The results are helpful in understanding the status of abandoned farmland in major grain-producing areas. It is believed they are significant for farmland protection and real-time national food security strategy.
Identifying climatically-compatible seedlots for the eastern US: building the predictive tools and knowledge to enable forest assisted migration
Authors: Adams Bryce T. , Royo Alejandro A. , Kern Christel C. , Bronson Dustin R. , Matthews Stephen N. , Gougherty Andrew V. , Prasad Anantha M. , Iverson Louis R. , Rehm Evan M. , Raymond Patricia , Périé Catherine , Miesel Jessica R. , McGraw Amanda M.
Published: November 2024

Introduction

Global climate change and associated stressors threaten forest ecosystems due to the rapid pace of climate change, which could exceed the natural migration rate of some tree species. In response, there is growing interest to research and implement forest assisted migration (FAM). Here, we used a species-independent indicator based on climate analogy, according to the sigma (dis)similarity (σd) index, to match planting sites across the eastern US with (future) climatically-compatible seedlots (CCS).

Methods

We developed CCS for a grid composed of 1 × 1° of latitude and longitude. CCS were based on future climate analogs with ≤2σd analogy to ensure CCS were representative of future climate change. CCS were located for three time periods, 2030's, 2050's, and 2090's and three emissions scenarios (SSP2-4.5, SSP3-7.0, and SSP5-8.5) from the Coupled Model Intercomparison Project phase 6 database, using 12 climate variables.

Results

CCS were identified for the majority of 1 × 1° grids based on the SSP3-7.0 scenario. Approximately 28% of 1 × 1° grid's 2090's projections included future climate novelty. The 2030's, 2050's, and 2090's CCS were located on average 222, 358, and 662 km or 1, 2, and 3 eastern seed zones away from the 1 × 1° grids, respectively. CCS were also located further south-southwest (188–197°). In addition, the average forest cover of CCS was approximately 2%, 5%, and 10% less than that of the 1 × 1° grids.

Discussion

Our development and synthesis of CCS emphasized four key results: (i) average distances to 2030's and 2050's CCS were similar to seed-transfer guidelines for some tree species, but 2090's CCS exceeded current recommendations; (ii) south-southwesterly locations of CCS aligned with tree species habitat distribution dynamics; (iii) future climate novelty potentially challenges the conceptual basis of FAM if tree species are not adapted to climate change; and (iv) variation in forest cover among CCS presents potential opportunities and challenges due the presence or absence of forestland to source seed. Ultimately, our goal was to locate and synthesize CCS that could enable FAM decision support.