bluuebunny/crossref_metadata_2025 huggingface dataset:
IDENTIDADE DE GÊNERO E REPRESENTAÇÕES DE MINORIAS NO ROMANCE DO FUNDO DO POÇO SE VÊ A LUA, DE JOCA REINERS TERRON
Authors: ZIBETTI Marilise , Teixeira PORTO Ana Paula
Published: June 2020

Este trabalho busca refletir sobre como se configura a construção da identidade de gênero do personagem-narrador transexual Wilson a partir da análise de lembranças da infância e adolescência do narrador apresentadas no romance Do fundo do Poço se vê a Lua, de Joca Reiners Terron, o qual foi publicado em 2010. O livro abarca questões de memória, esquecimento, morte, violência e busca da identidade de gênero, além de apontar a infância como espaço mitológico nos anos finais da ditadura militar brasileira. Considerando esse contexto, o enfoque da pesquisa é discutir ainda conflitos de sujeitos que vivem à margem da sociedade, como Wilson, que, para se livrar da sombra do irmão gêmeo, o violento William, e da identidade masculina, se metamorfoseia em Cleópatra. Para alcançar esses objetos, o estudo, de natureza bibliográfica, ampara-se em pressupostos teóricos sobre representações de minorias e de gêneros na literatura, com contribuições de teóricos como David Foster e Guacira Louro.
A MATERIALIDADE DA FORÇA POÉTICA IN CONCERT: LITERATURA PURA
Authors: VENTURA Gilson
Published: June 2020

Resumo: Seja em um grande evento musical popular, um concerto de apresentações clássica das sonatas de Corelli, Couperin ou Bach, ou ainda nos sons da moda de viola em volta das fogueiras nos mais rigorosos invernos. Não importa. Qualquer que seja o estilo ou a dimensão musical vê-se que ao assistirmos uma música executada no momento mesmo em que a consumimo-la, se observarmos mais aprofundadamente, perceberemos que há sim uma diferença entre essa música, produzida no momento exato de sua audição e aquela apenas ouvida no aparelho do quarto ou no rádio do caminhão. Ou ainda essa música executada e consumida instantaneamente será diferente daquela assistida pela ajuda da tecnologia pelos práticos DVDs. Essas três formas de ouvir música é o tema desse artigo que procurará analisar a magia do momento experimentado pelos expectadores de uma música que não foi gravada em áudio, tampouco em imagens, mas a música criada no aqui e agora. Tal análise far-se-á em harmonia da relação que há entre tal fenômeno e a Literatura.
O sentido da vida dos enfermeiros no trabalho em cuidados paliativos: revisão integrativa de literatura
Authors: Rocha Renata Carla Nencetti Pereira , Ramos Pereira Eliane , Silva Rose Mary Costa Rosa Andrade , Medeiros Angelica Yolanda Bueno Bejarano Vale de , Marins Aline Miranda da Fonseca
Published: June 2020

Objetivou-se identificar evidências científicas acerca do sentido da vida dos enfermeiros no trabalho em cuidados paliativos no contexto hospitalar numa perspectiva existencialista. Revisão integrativa conduzida nas bases indexadas LILACS, MEDLINE, BDENF, CINAHL e SCOPUS no período de 2008 a junho de 2019, seguindo as recomendações da ferramenta PRISMA. Foram selecionados 12 artigos internacionais dos quais emergiram três categorias temáticas: “experiências existenciais de enfermeiros que trabalham aos cuidados paliativos”, “os sentidos e significados atribuídos pelos enfermeiros em cuidados paliativos”, e “estratégias de enfrentamento e intervenções apontadas como superação de questões existenciais”. Para que o trabalho dos enfermeiros tenha sentido e represente um valor importante em sua vidas, torna-se importante conhecer as fontes motivadoras que orientam suas decisões, bem como a criação de janelas estratégicas no cuidado que promovam satisfação e desempenho no processo de trabalho e favoreça a cultura do cuidado a pacientes em cuidados paliativos.
ESTUDO EXPERIMENTAL DE SISTEMA DE ANCORAGEM POR CORDÃO DE FIBRAS DE CARBONO EM VIGAS REFORÇADAS AO CISALHAMENTO COM PRFC
Authors: Risson Adriano Vieira , Menon Nara Villanova
Published: June 2020

RESUMO: O presente trabalho tem como objetivo avaliar o comportamento estrutural de vigas de concreto armado, reforçadas à flexão com tecido de fibras de carbono, utilizando mecanismo de ancoragem composto pela inserção de cordão de fibras de carbono no concreto. Foram utilizadas cinco vigas com o objetivo de avaliar o mecanismo aplicado em reforços de cisalhamento. Todas as vigas foram ensaiadas à aplicação de carregamentos transversais e instrumentadas para avaliar a carga de ruptura, deslocamento vertical, deformação específica no aço, concreto e reforço e o modo de ruptura. Através dos resultados, verificou-se que o mecanismo de ancoragem estudado atuou impedindo que o elemento estrutural chegasse a ruína prematuramente por descolamento ou desprendimento do reforço. Foram constatados ganhos principalmente na ductilidade das vigas e no aumento de sua capacidade resistente, que chegou a apresentar acréscimos superiores a 300% das vigas sem reforço. Através deste resultado, concluiu-se que o sistema de ancoragem por inserção de cordão de fibras de carbono se mostrou eficiente para ambos os tipos de reforço.

ABSTRACT: This study aims to evaluate the structural behavior of reinforced concrete beams, reinforced with carbon fiber fabric, using an anchoring mechanism composed of carbon fiber strand inserted in concrete. Five beams were used in order to evaluate the mechanism applied in shear reinforcements. All beams were applied to the transverse loadings and instrumented to measure the breaking load, vertical displacement, deformation specifically in steel, concrete and reinforcement and failure mode. The results concluded that the studied anchoring mechanism acts to prevent the structural element from premature destruction through detachment or loosening of the reinforcement. The main improvements were made in the ductility of the beams and increase in their load capacity, which made an increase of more than 300% over unreinforced beams. Through this result, it was concluded that the mooring system by insertion of carbon fiber strand was efficient for both types of reinforcement.
VIGAS DE CONCRETO REFORÇADO COM FIBRAS DE AÇO SUBMETIDAS À FLEXÃO
Authors: Santos Valquiria Claret dos , GONÇALVES Paulo Cesar , Guimarães Adinele Gomes , Caballer Felipe Ott
Published: June 2020

RESUMO: O presente trabalho compara experimentalmente os resultados de ensaios à flexão a quatro pontos de vigas de concreto com e sem adição de fibras de aço. O objetivo principal do trabalho foi analisar as influências que as fibras de aço tendem em modificar o surgimento de patologias relacionadas à fissuração do concreto. Este trabalho se justifica pelo fato de que a implementação de elementos de reforço ao esforço de flexão não é amplamente explorada no Brasil, mesmo tendo ocorrido um crescimento considerável nas últimas décadas. Vigas com adição de fibras não é um método totalmente difundido no Brasil, entretanto pisos de concreto e lajes reforçados com fibras já são mais usuais nas construções do país Para análise do comportamento à flexão foram realizados ensaios de laboratório com oito corpos de prova, moldados com e sem fibras, a maioria com entalhe induzindo a propagação vertical da fissura na região central da viga. Foi utilizado um baixo teor de fibras de aço (0,25%) para não implicar na trabalhabilidade do concreto e, com isso, testar um material que pode ser confeccionado em situações usuais da construção civil. Para o teor de fibras estudado observou-se um acréscimo de 26,6% no valor da carga máxima atingida. Outro fator relevante foi que não houve dificuldades em relação a trabalhabilidade do concreto quando adicionado as fibras. Os resultados obtidos sobre a resistência comprovaram eficiência quando se usou as fibras no ensaio a flexão.
ABSTRACT: The present work compares the results experimentally of four-point bending tests of concrete beams with and without addition of steel fibers. The main objective of this work was to analyze the influence of steel fibers in modifying the appearance of pathologies related to concrete cracking. This work is justified by the fact that the implementation of reinforcement elements to the flexion effort is not widely explored in Brazil, even though there has been a considerable growth in the last decades. Beams with addition of steel fibers are not method used in Brazil, however, concrete floors and fiber-reinforced slabs are more common in the country's constructions. For bending behavior analysis, laboratory tests were performed with eight test specimens, molded with and without fibers, most of them with notch, inducing vertical propagation of the crack in the central region of the beam. It was use of a low content of steel fibers (0,25%) to avoid the workability of the concrete and, with this, to test a material that can be made in usual civil construction situations. For the fiber content studied, there an increase of 26,6% in the maximum load value. Another relevant factor was that there were no difficulties in relation to the workability of the concrete when added the fibers. The results obtained on the resistance proved efficiency when the fibers were used in the bending test.
O currículo cultural da Educação Física e os significados das práticas corporais: análise de uma prática pedagógica
Authors: De Castro Neta Abília Ana Ana , Boa Sorte Drieli Fernandes , Borges de Oliveira Glaurea Nádia , Costa Cardoso Berta Leni , Santana Cruz Marlon Messias
Published: June 2020

Esta investigação objetivou analisar as possíveis marcas do currículo cultural da Educação Física no modo como os alunos se relacionam com os significados das práticas corporais. Trata-se de uma pesquisa qualitativa, consubstanciada por uma intervenção pedagógica. Os sujeitos desta pesquisa foram os alunos do 4° Ano do Ensino Fundamental I, de uma escola pública do município de Guanambi-BA. É possível inferir que a problematização dos significados conferidos às práticas corporais tematizadas criou condições para hibridizar discursos e intercambiar conhecimentos. As significações inicialmente atribuídas às práticas investigadas foram sendo desestabilizadas, conquanto a legitimação dessa abertura dependa de circunstâncias que transcendem os limites da escola.
(In)visibilidades sociocomunicacionais na diáspora queer
Authors: Hadriel Theodoro , Cogo Denise
Published: June 2020

O objetivo deste artigo é refletir acerca das dinâmicas de (in)visibilidade inscritas nas experiências de sujeitos LGBTIQ+ (lésbicas, gays, bissexuais, travestis, transgêneros, transexuais, intersexo, queer e outras minorias de gênero e/ou sexuais) migrantes e como podem influir em suas interações sociocomunicacionais e no exercício de cidadania. Assim, elabora-se uma revisão de literatura com o intuito de contextualizar as particularidades do que se pode nomear de “diáspora queer” e sua interface com o binômio comunicação-cidadania. Em articulação com as reflexões teóricas desenvolvidas, propõem-se a sistematização e a análise de dados parciais de uma incursão empírica relacionada à imigração LGBTIQ+ na cidade de São Paulo, Brasil. A partir dessas duas vertentes (teórica e analítica), verifica-se a constituição de espaços e de dinâmicas de (in)visibilidade — institucionais e não institucionais — acerca da migração de sujeitos LGBTIQ+, o que possibilita pensar sobre sua existência e sobre as articulações para a garantia de direitos e para uma maior participação cidadã.
Part 1, Learning to Be Black: Unreconciled Strivings of DL and Black Gay Male Identity
Authors: Grant Alphonso Walter
Published: June 2020

While I was growing up, conversations about homosexual relationships were a taboo subject for as long as I can remember. It is a subject that burns deep within the heart and soul of the United States. For years, some DL and Black gay men have struggled to understand the Black community’s objection to the union of homosexual relationships. The term “DL,” which is short for “on the down low,” is a common way to refer to Black men who live their everyday lives in the guise of heterosexuals, are often married to women, yet also engage in discreet relationships with other men. This first-person narrative focuses on a time when I was employed with the Centers for Disease Control and Prevention (CDC) in New York City. I reflect on some of my interactions with DL and Black gay men. I discuss how some Black men who identify as gay are oftentimes hesitant to adopt the label “queer,” and to some extent even “gay,” due to the association of these terms with whiteness. This article is Part 1 in a series of two, both published in the same issue of Visual Arts Research. It is in direct conversation with the second article, “What It Means to Be Black: DL, Black, and Black Gay Male Images in Media—A Television Criticism. “ It is my intention to start this journey with “Learning to Be Black: Unreconciled Strivings of DL and Black Gay Male Identity” and end with an echoing negativity about how images of Black men are viewed and perceived in social media through a television criticism. These two articles are companion pieces, which feed off each other and rhythmically flow into a cohesive union of these most poignant and pressing topics of being DL, Black, and a gay man in the United States.

The history of the American Negro is the history of this strife,—this longing to attain self-conscious manhood, to merge his double self into a better and truer self. In this merging he wishes neither of the older selves to be lost. —W. E. B. Du Bois (1903)
Exploring the Implications of Melanie Yergeau’s Neuroqueer for Art Education
Authors: Attias Michelle
Published: June 2020

Yergeau’s concept of neurological queerness disrupts dominant cultural expectations of prosocial behaviors in the classroom, questioning how intention and sociality operate to remove the voices of neurodiverse individuals. Considering haptic engagement and material investigation in the arts classroom as potential sites for neurodiverse rhetorics to emerge challenges art educators to think about what access means within the classroom environment, as well as the narratives they ascribe to students’ asocial or antisocial behaviors. The neurologically queer have their own rhetorics of expression, which, if permitted to emerge within the arts classroom, create possibility for agency and expression. Art is a powerful rhetorical tool for self-advocacy and legibility, as it demonstrates what cannot be expressed through prosocial means. Counter-rhetorics of art and art making dismantle normative assumptions that privilege speaking and writing.
Part 2, What It Means to Be Black: DL, Black, and Black Gay Male Images in Media—A Television Criticism
Authors: Grant Alphonso Walter
Published: June 2020

This paper builds on the discourse in some of my previous research surrounding destabilizing whiteness—which is both a distinctiveness and a configuration together with who a white person is and the system of power that benefits white people and disadvantages Black people. In the intersection of Black male emasculation and sexuality, I focus on the sustained interaction of these two concepts with contemporary racial identity politics, masculinity, and media representation. I also reflect on categorization and institutionalization of Black men in visual culture. I discuss how power plays of hegemonic U.S. culture are structured and regulated by means of social dynamics. I ultimately provide insights into these things by investigating them and looking closely at their social constructs. I also focus on the social critique of power and marginality in terms of U.S. Black male culture. This manuscript is Part 2 in a series of two articles to show that a primary reason as to why DL and Black gay men and Black people are hated and feared is due to the ways in which we are negatively presented in television and media. These two manuscripts are companion pieces which feed off and rhythmically flow into each other to show a cohesive union. This manuscript sets the background out of concern and fear for the lives of DL, Black, and Black gay men in America in the 21st century.
The Influence of Technology on English Language and Literature
Authors: Abbasi Irum
Published: June 2020

In the current global scenario, the Internet is increasingly becoming a central informational medium that is transforming the way we learn, teach, and communicate. Social media offers a public platform that allows an exchange of thoughts and ideas through posts, tweets, and comments, albeit with word or character count restrictions. Evidently, creativity cannot be curtailed through content length restrictions. The emergence of a new genre of short-stories called short-short stories and the birth of a new English dialect called Text-speak prove that every cloud indeed has a silver lining. The popularity of social media exchanges signify that technology users have accepted quick social media interactions as a new way of life and have also adjusted their writing to match the content restrictions. Educators and parents are concerned that the attitudes and habits of tech-savvy generation are muddying Standard English as Text-speak is infiltrating students assignments blurring the distinction between formal and informal writing. The phenomenal popularity of short stories that can fit in a tweet or text is an example of how adversity can be turned into an opportunity. Literary purists, however, are concerned that digital literature is shrinking and short-stories are severing their characteristic elements to comply with the restrictions. This paper delineates the impact of technology on daily English writing and literature.
The Effect of Project-Based Learning Model on Persuasive Writing Skills of Saudi EFL Secondary School Students
Authors: Alotaibi Majed Ghazai
Published: June 2020

The aim of this study was to investigate the effect of project-based learning model on persuasive writing skills of Saudi EFL secondary students. The design of the study was quasi-experimental approach using pre and posttest for control and experimental groups. Participants were consisted of (59) students of third grade secondary school, randomly divided into two groups: experimental group consisted of (30) students and control group consisted of (29) students. Experimental group was taught using project-based learning while control group was taught using the traditional method. All participants were tested using the pre/post persuasive writing performance test before and after the intervention. The difference between the posttest's mean scores of the control and experimental groups were calculated using independent samples T-test. The result showed that there was a significant difference between the posttest's mean scores of the control and the experimental groups in favor of the experimental group. It was concluded that participants’ level of persuasive writing performance significantly developed after receiving the explicit project-based learning model.
Effects of Integrating Movie-Based Mobile Learning Instruction for Enhancing Thai University Students’ Speaking Skills and Intercultural Communicative Competence
Authors: Chaya Piyaporn , Inpin Bhornsawan
Published: June 2020

This research aimed to: 1) investigate the effects of Movie-Based Mobile Learning (MBML) instruction in enhancing Thai EFL university students’ speaking skills; 2) investigate effects of Movie-Based Mobile Learning instruction in enhancing Thai EFL university students’ intercultural communicative competence (ICC); and 3) examine the students’ attitude towards integrating Movie-Based Mobile Learning instruction for enhancing students’ speaking skills and intercultural communicative competence. This study employed a mixed-methods approach and a quasi-experimental study with a non-equivalent pretest-posttest control group design. The participants were the first year university students learning General English in the Faculty of Nursing at a private university in Bangkok. The students in the experimental group were taught through Movie-Based Mobile Learning instruction while the control group was taught following the university-based instruction. Research instruments consisted of MBML instruction with teaching materials, speaking test, the Intercultural Communicative Competence Questionnaire, Technology Acceptance Model (TAM) questionnaire, and the interview. The results revealed that Movie-Based Mobile Learning instruction developed Thai university students’ English speaking skills and Intercultural Communicative Competence, and that the students had positive attitude towards the implementing of MBML instruction in their English classroom.  The findings implied that MBML instruction is effective in enhancing EFL students’ speaking skills and intercultural communicative competence. The study suggests that relevant English language education should emphasize the potential of using the English movies and Mobile Learning in EFL curricula and that MBML instruction can be applied to promote learning in other similar EFL contexts.
The Construction of College English Online Learning Community under ADDIE Model
Authors: Zhang Jianfeng
Published: June 2020

Based on collaborative communication, reciprocal learning, and knowledge sharing, the college English online learning community, which is a kind of effective online learning method, can help to build knowledge construction and innovation. Therefore, from analysis, design, development, implementation and evaluation, ADDIE model is utilized to construct college online learning community that is fully combined with network environment, learners, teachers and learning resources, so that English learners’ integrative competences and the level of knowledge construction can be developed; meanwhile, the performance of college English online teaching will be promoted.
The Impact of Value-Belief-Norm Theory and Technology Acceptance Model on Use Intention of Green Design Packaging
Authors: Liu ChinHung , Wu YaHui
Published: June 2020

This study mainly investigates the relationships among VBN value-belief-norm (environmental moral obligation), technology acceptance model (perceived ease-of-use, perceived usefulness), and the intention to use designing sustainable packaging. A questionnaire survey was administered to consumers in various areas of Taiwan, and the responses were analyzed using statistical methods. The study results indicated the following: 1. The significant and positive impacts existed among VBN value-belief-norm (environmental moral obligation), perceived ease-of-use, perceived usefulness, and the intention to use sustainable packaging, with the influence of biospheric value in VBN on the new environmental paradigm being the most significant. 2. The consumers with various age and marital status had a significantly different perception on their intention to use designing sustainable packaging; the consumers with various age and occupation had a significantly different perception on their norms (environmental moral obligation), and when consumers purchased products that require packaging, whether they chose packaging with recyclable or reusable marks and functions had a significantly different perception on their VBN value-belief-norm, perceived ease-of-use, perceived usefulness, and intention to use designing sustainable packaging. Based on the analysis results above, we put forward suggestions regarding the recycling and reuse of resources, sustainable development technologies, and the cherishing of the ecological value of nature from the perspectives of consumers, companies, and the government.
Naoorlogs universalisme in het huidige socialezekerheidsdebat
Authors: Brink Barbara , Vonk Gijsbert
Published: June 2020

Post-war universalism in the current social security debate

The Western European social security systems are founded on the need to offer universal social protection, as was for example advocated in the Beveridge report of 1942. The universalistic endeavour has led to the development of the all-embracing welfare states of today, but already for many decades dissatisfaction with the direction of the welfare state has led to a diversion of the universalistic pretention. In the current debate, universalism seems to be on the rise again. The Dutch think tanks CPB, WRR and SCP increasingly pay attention to the divide that is becoming manifest between those with better chances in the society and who are left behind. The think tanks have all formulated policy options in order to address this divide by offering better social security protection for excluded groups. In this article we discuss whether the options presented fall back upon the post-war notion of universality.
Naar nieuwe scheidslijnen op de Nederlandse arbeidsmarkt? Een pleidooi voor uitvoerbaarheid van beleid
Authors: Versantvoort Maroesjka , Putters Kim
Published: June 2020

Towards new dividing lines on the Dutch labour market? A plea for policy feasibility

Recent developments on the Dutch labour market raise questions about the emergence of new social dividing lines. In this paper we discuss two cases. Both address labour market disadvantages which can easily deepen and sharpen with unchanged policies. The first case shows that there are groups that are less able to respond to the defined trends in the labour market due to a lack of education or disabilities. In the second case we focus on the question of sustainable employability as such that creates dividing lines. A first conclusion from our contribution is that a thorough analysis of existing dividing lines in the labour market is crucial for effective policy, but that knowledge about deepening old and creating new dividing lines is at least as relevant. A second conclusion is that the outlined assumptions in policies around self-reliance of, for example, people with a disability or the possibilities of employers to realize sheltered work and to focus on retraining, for example outside working hours, are not always realistic. Much more will have to be done to match the real possibilities of people and organizations to achieve this. Financial, but also organizational and personal. The motivations and behaviors of employees, employers and educational authorities appear to be very relevant explanatory factors in practice, which are often not sufficiently taken into account. Recent experiences with the Participation Act and lifelong learning show us that. More interaction is needed in the coming years between research, policy and the practice of the labour market.
Het betere werk: inzetten op de kwaliteit van de arbeid
Authors: Kremer Monique , Went Robert
Published: June 2020

Better work: society’s new mission

New technology, flexibilization and the intensification of work could have significant consequences for those who still have work in the future, and for the quality of that work. In a new report, the Netherlands Scientific Council for Government Policy (WRR) is therefore arguing that good work for everyone should now be seen as an important aspiration for companies, institutions, social partners and the government. Good work is essential for general well-being: for the individual’s quality of life, for the economy and for society as a whole. We make nine policy proposals to promote and facilitate good work for more people.
Schaal en invloed
Authors: Steur Boudewijn
Published: June 2020

Scale and influence – Plea for a symbiosis of indirect and direct democracy

In a democracy citizens should actually have influence on the choices that directly influence their lives. Citizens have two ways for this influence: directly by participating in the policy process (in its formulation, its decision making or its implementation) or indirectly by voting for a political party or representatives through which citizens have influence on the outcomes. These two are not opposite to each other, but rather complementary. My main argument in this article is that the smaller the scale, the greater the possibilities for citizens to exert direct influence. The larger the scale, the more important it is that this influence runs through their representative institutions
Zaken oplossen met behulp van de DNA-databank: een pleidooi voor een brede inhoudelijke discussie over de voorgenomen wijziging van de Wet DNA-V
Authors: Goos M. , Stevens L.
Published: June 2020

Zaken oplossen met behulp van de DNA-databank: een pleidooi voor een brede inhoudelijke discussie over de voorgenomen wijziging van de Wet DNA-V

Op grond van de Wet DNA-onderzoek bij veroordeelden (Wet DNA-V) worden DNA-celmateriaal en DNA-profielen van veroordeelden bewaard. Omdat sommige veroordeelden onvindbaar blijken voor de afname van hun celmateriaal zal vermoedelijk de wet worden aangepast opdat conservatoire afname mogelijk wordt. DNA wordt dan reeds afgenomen tijdens het opsporingsonderzoek maar pas verwerkt na veroordeling. Deze bijdrage plaatst de discussie over de juridische en praktische houdbaarheid van de conservatoire afname in een breder perspectief, namelijk die van de oorspronkelijke doelstellingen van de Wet DNA-V en de vraag naar de mogelijkheden van het afnemen én het reeds verwerken van DNA-celmateriaal tijdens het opsporingsonderzoek.
Herstelbeslissingen in het nieuwe Wetboek van Strafvordering nader beschouwd
Authors: Haentjens R.C.P.
Published: June 2020

Herstelbeslissingen in het nieuwe Wetboek van Strafvordering nader beschouwd

De voorstellen tot modernisering van het nieuwe Wetboek van Strafvordering betreffen onder meer nieuwe bepalingen aangaande herstelbeslissingen door de strafrechter na sluiting van het onderzoek ter terechtzitting. De voorstellen codificeren voor een groot gedeelte de reeds tot ontwikkeling gekomen jurisprudentie. Nieuw is het gemarkeerde onderscheid tussen het aanbrengen van verbeteringen en het aanvullen van de rechterlijke beslissing. Dit onderscheid werkt door in de uitwerking wat betreft de procedurele bevoegdheden van de bij het strafproces betrokken partijen: de strafrechter, de Officier van Justitie, de verdachte, de benadeelde partij en in het voorkomende geval de (voogd van de) minderjarige. De voorgestelde nieuwe regeling roept vragen op met betrekking tot de beginselen van concentratie en geslotenheid van rechtsmiddelen. Tevens vraagt de regeling voor de aanvulling nadere bezinning: tot welk moment is aanvulling toegelaten met het oog op het aanweneden van rechtsmiddelen? De vraag is gerechtvaardigd of de regeling zoals voorgesteld niet nog eens kritisch moet worden bezien. In het hierna volgende artikel wordt die vraag bevestigend beantwoord.
The evaluation of environmental and socio-cultural impacts of tourism on the Khorgo-Terkhyn Tsagaan Nuur National Park
Authors: Ulaankhuu Khishigdalai
Published: June 2020

The Khorgo-Terkhiin Tsagaan Nuur National Park is one of the major tourist destinations in Mongolia. The Park, in recent years, has witnessed a significant increase in the number of visiting local tourists. The present research was conducted to assess the environmental, social and cultural impacts of tourism in the region particularly today when we talk about sustainable tourism. To assess these impacts, as assessment method was used which is applied internationally. These impacts were based on three criterion for sustainable development-environmental impacts, social and cultural impacts. The National Park has been facing environmental impacts such as poor waste management, soil erosion, overgrazing, and water pollution. Even though it has some great impacts on the social, cultural and environmental aspects and its products and services, preserving traditional and cultural heritages and improving the skills of local and tourist workers, it continues to have a negative affect on the traditional customs, national arts and culture. Therefore, it is important that the means and methods of reducing these negative impacts should be planned and developed in the near future.
Estimation of the Active Social Network of Street Children in Shiraz (A City of Iran)
Authors: Motazedian Nasrin , Sayadi Mehrab , Oboodi Somaye , Joulaei Hassan
Published: June 2020

: Street children are a hard-to-reach population. Since the direct method is not feasible and has some limitations, we utilized the network scale-up (NSU) as an indirect method. This study was conducted in Shiraz municipalities. Our target population was Iranian boys between 10 to 18 years of age during the years 2014 to 2016. Three trained psychologists conducted face to face interviews with 86 street children (boys) on the streets, through the convenience sampling technique. The social network size of street boys in Shiraz was estimated at 17 persons (ranging from zero to 92 people, mean = 17 ± 17, median = 13). Overall, the network size of the hidden population might be smaller than the general population, due to the stigmatized nature of their behavior and place of work.
Emergency Field Hospital Following Kermanshah Earthquake in 2017: A Report of an Experience
Authors: Farahmand Faramarz , Hajipour Saeid , Mousavi-Roknabadi Razieh Sadat , Sajjadi Seyed Mahmoudreza , Dehbozorgi Afsaneh , Sharifi Mehrdad
Published: June 2020

Background: Following an earthquake with a magnitude of 7.3 on the Richter scale took place in the west of Iran, on November 12th, 2017, numerous state and community response teams were dispatched for a variety of purposes. Objectives: This study aimed to describe the experiences of the medical team dispatched from Shiraz University of Medical Sciences (SUMS) in an emergency field hospital in the city of Sarpol-e-Zahab following the Kermanshah earthquake. Methods: In this cross-sectional study, the data collected by SUMS’s team were used. In this regard, the condition of the only local hospital in Sarpol-e-Zahab after the earthquake, the actions taken by SUMS’s team, the existing problems, as well as characteristics of the visited patients, were described. Finally, the satisfaction of the medical staff at the hospital under the study with the performance of the SUMS’s team was analyzed. Results: Following the earthquake, the only hospital in Sarpol-e-Zahab became semi-open. An emergency field hospital was partially established in the hospital’s parking lot. The SUMS’s team changed the arrangement of the field hospital, and during eight days, visited more than 9,000 patients. The most frequent chief complaints of patients were signs of the flu and respiratory infections, various types of trauma, and gastrointestinal problems. Conclusions: Our results indicated that the data were not adequate to cover all the aspects involved. This shows the necessity of developing a registry system for better management of healthcare at times of the crisis.
Modulation of dopamine D1 receptors via histamine H3 receptors is a novel therapeutic target for Huntington's disease
Authors: Moreno-Delgado David , Puigdellívol Mar , Moreno Estefanía , Rodríguez-Ruiz Mar , Botta Joaquín , Gasperini Paola , Chiarlone Anna , Howell Lesley A , Scarselli Marco , Casadó Vicent , Cortés Antoni , Ferré Sergi , Guzmán Manuel , Lluís Carmen , Alberch Jordi , Canela Enric I , Ginés Silvia , McCormick Peter J
Published: June 2020

Early Huntington’s disease (HD) include over-activation of dopamine D1 receptors (D1R), producing an imbalance in dopaminergic neurotransmission and cell death. To reduce D1R over-activation, we present a strategy based on targeting complexes of D1R and histamine H3 receptors (H3R). Using an HD mouse striatal cell model and HD mouse organotypic brain slices we found that D1R-induced cell death signaling and neuronal degeneration, are mitigated by an H3R antagonist. We demonstrate that the D1R-H3R heteromer is expressed in HD mice at early but not late stages of HD, correlating with HD progression. In accordance, we found this target expressed in human control subjects and low-grade HD patients. Finally, treatment of HD mice with an H3R antagonist prevented cognitive and motor learning deficits and the loss of heteromer expression. Taken together, our results indicate that D1R - H3R heteromers play a pivotal role in dopamine signaling and represent novel targets for treating HD.
Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma
Authors: Hastings Jordan F , Gonzalez Rajal Alvaro , Latham Sharissa L , Han Jeremy ZR , McCloy Rachael A , O'Donnell Yolande EI , Phimmachanh Monica , Murphy Alexander D , Nagrial Adnan , Daneshvar Dariush , Chin Venessa , Watkins D Neil , Burgess Andrew , Croucher David R
Published: June 2020

The identification of clinically viable strategies for overcoming resistance to platinum chemotherapy in lung adenocarcinoma has previously been hampered by inappropriately tailored in vitro assays of drug response. Therefore, using a pulse model that closely mimics the in vivo pharmacokinetics of platinum therapy, we profiled cisplatin-induced signalling, DNA-damage and apoptotic responses across a panel of human lung adenocarcinoma cell lines. By coupling this data to real-time, single-cell imaging of cell cycle and apoptosis we provide a fine-grained stratification of response, where a P70S6K-mediated signalling axis promotes resistance on a TP53 wildtype or null background, but not a mutant TP53 background. This finding highlights the value of in vitro models that match the physiological pharmacokinetics of drug exposure. Furthermore, it also demonstrates the importance of a mechanistic understanding of the interplay between somatic mutations and the signalling networks that govern drug response for the implementation of any consistently effective, patient-specific therapy.
Existence and functions of a kisspeptin neuropeptide signaling system in a non-chordate deuterostome species
Authors: Wang Tianming , Cao Zheng , Shen Zhangfei , Yang Jingwen , Chen Xu , Yang Zhen , Xu Ke , Xiang Xiaowei , Yu Qiuhan , Song Yimin , Wang Weiwei , Tian Yanan , Sun Lina , Zhang Libin , Guo Su , Zhou Naiming
Published: June 2020

The kisspeptin system is a central modulator of the hypothalamic-pituitary-gonadal axis in vertebrates. Its existence outside the vertebrate lineage remains largely unknown. Here, we report the identification and characterization of the kisspeptin system in the sea cucumber Apostichopus japonicus. The gene encoding the kisspeptin precursor generates two mature neuropeptides, AjKiss1a and AjKiss1b. The receptors for these neuropeptides, AjKissR1 and AjKissR2, are strongly activated by synthetic A. japonicus and vertebrate kisspeptins, triggering a rapid intracellular mobilization of Ca2+, followed by receptor internalization. AjKissR1 and AjKissR2 share similar intracellular signaling pathways via Gαq/PLC/PKC/MAPK cascade, when activated by C-terminal decapeptide. The A. japonicus kisspeptin system functions in multiple tissues that are closely related to seasonal reproduction and metabolism. Overall, our findings uncover for the first time the existence and function of the kisspeptin system in a non-chordate species and provide new evidence to support the ancient origin of intracellular signaling and physiological functions that are mediated by this molecular system.
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons
Authors: Vevea Jason D , Chapman Edwin R
Published: June 2020

The success of comparative cell biology for determining protein function relies on quality disruption techniques. Long-lived proteins, in postmitotic cells, are particularly difficult to eliminate. Moreover, cellular processes are notoriously adaptive; for example, neuronal synapses exhibit a high degree of plasticity. Ideally, protein disruption techniques should be both rapid and complete. Here, we describe knockoff, a generalizable method for the druggable control of membrane protein stability. We developed knockoff for neuronal use but show it also works in other cell types. Applying knockoff to synaptotagmin 1 (SYT1) results in acute disruption of this protein, resulting in loss of synchronous neurotransmitter release with a concomitant increase in the spontaneous release rate, measured optically. Thus, SYT1 is not only the proximal Ca2+ sensor for fast neurotransmitter release but also serves to clamp spontaneous release. Additionally, knockoff can be applied to protein domains as we show for another synaptic vesicle protein, synaptophysin 1.
Cohesion is established during DNA replication utilising chromosome associated cohesin rings as well as those loaded de novo onto nascent DNAs
Authors: Srinivasan Madhusudhan , Fumasoni Marco , Petela Naomi J , Murray Andrew , Nasmyth Kim A
Published: June 2020

Sister chromatid cohesion essential for mitotic chromosome segregation is thought to involve the co-entrapment of sister DNAs within cohesin rings. Although cohesin can load onto chromosomes throughout the cell cycle, it only builds cohesion during S phase. A key question is whether cohesion is generated by conversion of cohesin complexes associated with un-replicated DNAs ahead of replication forks into cohesive structures behind them, or from nucleoplasmic cohesin that is loaded de novo onto nascent DNAs associated with forks, a process that would be dependent on cohesin’s Scc2 subunit. We show here that in S. cerevisiae, both mechanisms exist and that each requires a different set of replisome-associated proteins. Cohesion produced by cohesin conversion requires Tof1/Csm3, Ctf4 and Chl1 but not Scc2 while that created by Scc2-dependent de novo loading at replication forks requires the Ctf18-RFC complex. The association of specific replisome proteins with different types of cohesion establishment opens the way to a mechanistic understanding of an aspect of DNA replication unique to eukaryotic cells.
An ECF-type transporter scavenges heme to overcome iron-limitation in Staphylococcus lugdunensis
Authors: Jochim Angelika , Adolf Lea , Belikova Darya , Schilling Nadine Anna , Setyawati Inda , Chin Denny , Meyers Severien , Verhamme Peter , Heinrichs David E , Slotboom Dirk J , Heilbronner Simon
Published: June 2020

Energy-coupling factor type transporters (ECF) represent trace nutrient acquisition systems. Substrate binding components of ECF-transporters are membrane proteins with extraordinary affinity, allowing them to scavenge trace amounts of ligand. A number of molecules have been described as substrates of ECF-transporters, but an involvement in iron-acquisition is unknown. Host-induced iron limitation during infection represents an effective mechanism to limit bacterial proliferation. We identified the iron-regulated ECF-transporter Lha in the opportunistic bacterial pathogen Staphylococcus lugdunensis and show that the transporter is specific for heme. The recombinant substrate-specific subunit LhaS accepted heme from diverse host-derived hemoproteins. Using isogenic mutants and recombinant expression of Lha, we demonstrate that its function is independent of the canonical heme acquisition system Isd and allows proliferation on human cells as sources of nutrient iron. Our findings reveal a unique strategy of nutritional heme acquisition and provide the first example of an ECF-transporter involved in overcoming host-induced nutritional limitation.
Relationship between the average slope in the active commuting to and from school and fitness in adolescents: the mediator role of fatness
Authors: Sánchez Miguel Pedro Antonio , Sánchez Oliva David , Vaquero Solís Mikel , Pulido J. J. , Tapia Serrano Miguel Angel
Published: June 2020

Active commuting to and from school (ACS) has been recognized as a potential tool to improve physical fitness. Thus, this study aims to test the relationships between the average slope in the ACS and physical fitness, as well as to verify the mediator role of fatness in the relationship between average slope and physical fitness. A total of 257 participants, 137 boys and 120 girls, from 22 schools belonged to first and second High School grades participated in this study. Based on self-reported measure and Google Earth, participants were grouped into the active commuter (number of trips was ≥5, and the time of the trip was ≥15 min), mixed commuter (number of weekly trips was <5, and the time spent on the trip was <15 min) and passive commuter groups (those who reported traveling regularly by car, motorcycle, or bus). Specifically, in the active commuter group, a positive association between the average slope in the ACS with fatness was found, which in turn was positively related to strength lower limbs and cardiorrespiratory fitness. The average slope was not significantly associated with physical fitness indicators. Furtheremore, fatness did not mediate the relathionship between average slope and physical fitness. This research concluded positive associations between average slope and the body fat in the ACS. The tendency of findings signal that the average slope should be taken into account along with the distance, time and frequency of the active commuting.
Validation of a general subjective well-being factor using Classical Test Theory
Authors: Nima Ali Al , Cloninger Kevin M. , Lucchese Franco , Sikström Sverker , Garcia Danilo
Published: June 2020

Background

Subjective Well-Being (SWB) is usually conceptualized in terms of an affective (i.e., judgements of biological emotional reactions and experiences) and a cognitive component (i.e., judgements of life satisfaction in relation to a psychological self-imposed ideal). Recently, researchers have suggested that judgements of harmony in life can replace or at least complement the cognitive component of SWB. Here, however, we go beyond that suggestion and propose that harmony in life should be seen as SWB’s social component since it is the sense of balance between the individual and the world around her—a process that comprises acceptance, adaptation, and balance. By adding judgements of one’s social interactions (i.e., harmony in life) to judgments of one’s life satisfaction (psycho) and judgements of one’s emotional reactions (bio), we propose a tentatively biopsychosocial model of SWB. As a first step, we used different factorial models in order to determine if both a general factor and specific sub-factors contribute to the biopsychosocial model of SWB.

Method

A total of 527 participants responded to the Positive Affect Negative Affect Schedule (PANAS; 20 items), the Satisfaction with Life Scale (SWLS; five items), and the Harmony in life Scale (HILS; five items). We conducted exploratory and confirmatory factor analyses to validate the biopsychosocial model of subjective well-being and a general factor (SWBS).

Results

The 20 PANAS items reflected a mixture of general latent structure saturation and specific latent structure saturation, but contributed to their respective specific latent factor (PA: 48%; NA: 49%) more than to the general latent SWBS factor (positive affect: 25%; negative affect: 32%). The five SWLS items contributed to a larger degree to the general SWBS factor (72%) than to life satisfaction itself (22%), while the five HILS items contributed to even a larger degree to the general SWBS factor (98%) than to harmony in life (0%). The bifactor model was the best model compared with all other models we tested (χ2= 1,660.78, df = 375,p< 0.001); Satorra Bentler χ2= 1,265.80, df = 375,p< 0.001; CFI = 0.92; Tucker–Lewis Index = 0.91; RMSEA = 0.067. This model of a general SWBS factor explained about 64% of the total variance in the model, while specific SWBS components together explained 15% of the total variance.

Conclusion

Our study suggests SWB as a general factor in a multidimensional biopsychosocial model. Indeed, as much as 64% of the variance of SWB was explained by this general factor. The SWB components, however, contributed to a different degree to each corresponding factor in the model. For instance, while the affective and cognitive components seem to be their own constructs and also part of the general SWB factor, the social component tested here contributed 0% to its own variance but 98% to the general factor.