bluuebunny/crossref_metadata_2025 huggingface dataset:
Physical Therapy as Part of a Telehealth Multidisciplinary Lifestyle Modification Program (IM FIT): Preliminary Outcomes and Program Feedback From Cancer Survivors
Authors: Brehm Christin A. , Christie Aimee J. , Cohen Lorenzo , Lilly Susan , Powers-James Catherine , Lopez Gabriel
Published: November 2024

Background:

Physical therapy (PT) has an important role in Integrative Oncology programs, addressing the unique physical health needs of individuals with cancer through exercise counseling. We share our experience with the PT component of our telehealth comprehensive lifestyle modification program including data from our first cohort of patients.

Methods:

The PT component of our lifestyle modification program included 6 one-hour synchronous video sessions (30 minutes exercise education, 30 minutes exercise) over the 12-week program. Participants received handouts electronically that included information regarding each week’s exercise-related topic, as well as weekly exercise assignments. Outcomes included weight, days of aerobic exercise, minutes of aerobic exercise, and days of strengthening exercise. Six patients completed the program and submitted an anonymous exit survey providing qualitative feedback on the program.

Results:

On average, patients reported engaging in aerobic exercise 4 days per week and strength training 2 days per week after the intervention. There were notable increases in days of strength training. Patients lost an average of 4.2% of their body weight over the course of the 12-week program. Qualitative feedback showed that the first cohort of patients described the telehealth program as enjoyable, valuable, and convenient.

Discussion:

Physical therapy exercise programs can be modified and tailored for delivery in a telehealth environment. Increased patient access remains a strength of this model, serving to equip patients with the tools needed for increasing aerobic and strength training activity. Overall, the program was implemented successfully, and the anonymous feedback survey completed after program completion showed high patient satisfaction.
Assessing the Use of Virtual Pointers in Laparoscopic Surgery Training Activities
Authors: Jiménez-Ruescas Javier , Celdrán Fco J. , Salazar Lucía , Sánchez-Margallo Juan A. , Sánchez-Margallo Francisco M. , González Pascual
Published: November 2024

Background

Laparoscopic surgery has advanced significantly; however, effective communication within the surgical team remains challenging, particularly in training scenarios where misinterpretation of instructions can lead to errors. Virtual pointers, indicating the instructor’s gaze through eye-tracking devices and overlaying it onto the screen using augmented reality (AR), can enhance understanding and reduce errors.

Methods

In a study involving 10 participants divided into experts and novices in laparoscopic surgery, various pointers (differing in shape, size and colour) were analysed in an AR environment using the HoloLens2 device. The aim was to assess the acceptance of these pointers by both groups, understand how their characteristics affect perception, and evaluate the AR device’s usefulness.

Results

Initial results showed an 80% acceptance rate. After analysing the pointers, this decreased to 60%, as some experts changed their opinion from positive to neutral. The study revealed that the characteristics of the pointers significantly affect their perception, with large, thick-rimmed pointers being preferred. Orange was the best-performing colour. The device used also influenced perception, as experts who changed their perception criticised certain aspects of the head-mounted display.

Conclusions

The study highlights the potential of virtual pointers to improve communication in laparoscopic training, particularly when their size, shape, and colour are optimised. Further research with a larger sample size is needed to confirm these results and explore the HoloLens2’s influence on users’ perceptions. These insights could guide the development of more effective training tools for minimally invasive surgery.
Unreal Games
Authors: Therrien Carl , Ray Jean-Charles , Ross Dionne Laurie-Mei , Sacy Fabienne
Published: November 2024

Throughout the history of game studies, scholars have reflected on the essential features of games. In the past decade, these discussions have extended beyond academia to the broader gaming community, where certain genres have been elevated as “real games.” Walking simulators, dating sims, visual novels, and hybrid forms are often at the center of these debates, criticized for their simplicity, lack of challenge, or sometimes overtly sexual content. Despite this, the popularity of these genres is undeniable: since 2007, over 50,000 titles have been documented on the Visual Novel Database, and thousands of walking simulators are available on Steam and other platforms. Many of these titles also appear on MobyGames and have been referenced as “games” throughout history. In this article, we delve into this vast and often overlooked corpus to examine the qualities that can elevate gameplay to unreal levels.
Thirty-day outcomes of endovascular repair of Stanford type B aortic dissection in patients with chronic obstructive pulmonary disease
Authors: Li Renxi , Sidawy Anton , Nguyen Bao-Ngoc
Published: November 2024

Background

Thoracic Endovascular Aortic Repair (TEVAR) has revolutionized the surgical treatment for Stanford type B aortic dissection (TBAD). While chronic obstructive pulmonary disease (COPD) is associated with worse outcomes in major surgeries, the specific outcomes of TEVAR in patients with COPD have not been extensively explored. This study aimed to evaluate the 30-day postoperative outcomes of COPD patients undergoing TEVAR for TBAD utilizing data from a multi-institutional national registry.

Methods

Patients who underwent TEVAR for TBAD were identified in the ACS-NSQIP database from 2005 to 2022. A 1:3 propensity-score matching was used to match demographics and preoperative characteristics between patients with and without COPD. Thirty-day postoperative outcomes were compared.

Results

There were 172 (9.56%) and 1628 (90.44%) COPD and non-COPD patients who underwent TEVAR for TBAD, respectively. Patients with COPD had a higher comorbidity burden. After the propensity-score matching, all 172 COPD patients were matched to 440 non-COPD patients. COPD and non-COPD patients had comparable mortality rates (10.12% vs 6.82%, p = .18). However, COPD patients had a higher risk of pulmonary complications (20.83% vs 13.18%, p = .02). All other 30-day outcomes were similar between the two groups.

Conclusion

COPD patients had 58.04% higher pulmonary complications while all other 30-day outcomes were comparable to their non-COPD counterparts. Therefore, close monitoring and timely intervention for pulmonary complications in COPD patients can be important after TEVAR for TBAD. Future studies should investigate long-term outcomes among these COPD patients.
Prevalence of urinary tract infections in pregnancy in rural Andean communities of Peru
Authors: Venkatesh Apoorva , Sanchez-Samaniego Giuliana , Mäusezahl Daniel , Hattendorf Jan , Hartinger Stella Maria
Published: November 2024

Background:

Urinary tract infections (UTIs) can adversely affect pregnancy, yet their prevalence in rural communities remains poorly understood.

Objective:

For an initial evaluation of the UTI burden among pregnant women in the San Marcos province of rural Andean Peru, we aimed to determine the UTI prevalence in the region.

Design:

A cross-sectional study was conducted in a subsample of 250 pregnant women enrolled in the Peruvian Andes Multigenerational High Altitude Cohort (ALTO) from 2021 to 2022.

Methods:

Structured questionnaires were administered to collect demographic, socioeconomic, maternal health and behavioural data. Urine samples were obtained for dipstick analyses. Descriptive and logistic regression analyses were performed to investigate the association between risk factors and UTI.

Results:

The study participants had a median age of 28 years (IQR: 22–33). A UTI prevalence of 27.6% (CI: 22.4%–33.5%) was observed, surpassing estimates from other regions of Peru. Notably, nearly all pregnant women (96%) utilised antenatal care (ANC) services at least once, primarily visiting health centres and community health posts where doctors and obstetricians were the main care providers. In this population, none of the risk factors exhibited statistically significant associations with UTIs.

Conclusion:

Our study highlights the prevalence of UTIs among Andean pregnant women in San Marcos and underscores the critical need for routine UTI screening and treatment during ANC visits as recommended by national guidelines. While accessibility to ANC services is not a barrier in this region, enhancing the availability and quality of UTI screening services is crucial.
“Unwanted guests” or welcomed neighbors? Portrayals of Ukrainian refugees in Russian, Polish, and UK news coverage
Authors: Roman Nataliya , Myslik Barbara , Beasley Berrin
Published: November 2024

The 2022 Russian invasion of Ukraine forced more than eight million Ukrainians to seek refuge outside their home country. This study analyzed the press coverage of displaced Ukrainians during the first year of the war in three countries that resettled some of the largest numbers of Ukrainian refugees: Poland, Russia, and the UK. The researchers found important differences in how news organizations from the three countries portrayed Ukrainian refugees. Polish and UK coverage was mainly neutral and sympathetic toward Ukrainian refugees, while Russian coverage depended on where stories were set geographically.
Considerations for regulation and evaluation of digital mental health technologies
Authors: Hopkin Gareth , Branson Richard , Campbell Paul , Coole Holly , Cooper Sophie , Edelmann Francesca , Salmon Mark
Published: November 2024

Digital mental health technologies (DMHTs) are becoming well established within mental health services and through direct-to-consumer models. Due to their scalable nature, DMHTs may support services to bridge the gap between demand and the available workforce, particularly where existing pathways have long delays or restricted capacity. Challenges and risks associated with DMHTs also need consideration. Regulatory and health technology assessment (HTA) agencies play a key role in ensuring the potential of DMHTs is achieved and their risks are mitigated. However, the nature of DMHTs and advances in digital technology present challenges for regulation and evaluation.

In this paper, we describe eight key considerations across the regulatory and HTA pathway for DMHTs. These relate to 1) intended purpose, 2) qualification and classification, 3) risk management, 4) clinical evidence, 5) resource requirements and economic evidence, 6) post-market surveillance and life cycle assessment, 7) replicability and equity, and 8) wider responsibilities. Ensuring clarity within these considerations and addressing outstanding uncertainties is necessary to ensure that the benefits of DMHTs are unlocked, while also ensuring that people have access to high quality and safe tools.
A Rare Case of Solitary Cardiac Myofibroma in an Infant
Authors: Yu Liwei , O’Leary Patrick W. , Qureshi M. Yasir , Maleszewski Joseph J. , Stephens Elizabeth H. , Dearani Joseph A. , Ameduri Rebecca K.
Published: November 2024

Solitary myofibromas are rare, benign, mesenchymal tumors typically found in the head and neck regions. We report an exceptionally rare case of a solitary cardiac myofibroma and its short-term management and outcome. A two-month-old infant was referred due to a murmur. Echocardiography revealed a 2 × 1 cm solitary mass originating from a mitral papillary muscle, causing severe regurgitation. Magnetic resonance imaging showed the mass to be homogeneous on T1, hyperintense on T2, and hypointense on first-pass perfusion imaging with no enhancement on delayed postcontrast imaging. The mass was surgically excised partially due to its involvement with the mitral valve. Postoperative recovery was uneventful, with echocardiography four months later showing no tumor recurrence and trivial mitral regurgitation. This report provides valuable imaging and pathology examples to aid in the recognition of this rare tumor and demonstrates that successful surgical excision is possible, even with significant cardiac involvement.
Are radical changes to lifestyles necessary for mitigating climate change?
Authors: Whitmarsh Lorraine , Hampton Sam
Published: November 2024

Many policy-makers and commentators propose that technological change alone will solve climate change. In this commentary, we argue that this techno-optimism is unrealistic, risky, and misses opportunities to improve well-being: radical behavioural change is essential for tackling climate change. While some behaviour change involves adopting green technologies, some are about changing how (much) we consume. Change is needed in our travel and dietary habits, material, energy and water use, in how we work and play. But not everyone needs to make radical lifestyle changes – the wealthiest need to change most. Achieving this will require policies that make low-carbon behaviours easier, cheaper, and more attractive. Tackling climate change requires a shift to a more equitable society and one in which happiness is prioritised over consumption.
High-Speed and Low-Noise Gear Finishing by Gear Grinding and Honing: A Review
Authors: Tian Xiaoqing , Sun Yukai , Mu Weihao , Xia Lian , Han Jiang
Published: November 2024

Gears are important mechanical parts for transmitting power and play an increasingly important role in the machinery industry. The electric vehicle industry developed rapidly and became a vital development field of the automotive industry over the past few years. The conversion of energy increases the requirements for electric vehicle transmissions, which promotes the development of high-speed and low-noise transmission gear. This paper elaborates on the research progress of high-speed and low-noise gear finishing methods. Firstly, this review analyses the machining requirements, finishing, and specific machining technologies of high-speed and low-noise gears. The importance of gear grinding and honing in high-speed and low-noise gear machining is highlighted. Secondly, the applications of gear grinding and honing in gear modification, error compensation, and texture control are analyzed. Furthermore, the role of precision machining technology in improving gear performance is clarified. Finally, the design and processing methods of the modified tooth flank for gear modification are summarized. In addition, the influence of tooth surface texture on noise is described, and the texture change methods are explored. The well-known open problems of gear finishing are finally identified, and some new research interests are also pointed out. This review will provide valuable references for further research on gear finishing.
The effect of anxiety and autism symptom severity on restricted and repetitive behaviors over time in children with fragile X syndrome
Authors: Moskowitz Lauren J. , Will Elizabeth A. , Black Conner J. , Roberts Jane E.
Published: November 2024

Background

Restricted and repetitive behaviors (RRBs) are highly prevalent and reduce function in individuals with fragile X syndrome (FXS). As transdiagnostic features of intellectual disability, elevated rates of RRBs in FXS could represent various underlying known co-occurring conditions in FXS such as anxiety or autism spectrum disorder (ASD), yet this distinction has not been investigated. Further, delineating whether RRBs are more indicative of anxiety or ASD in FXS may clarify phenotypic profiles within FXS and improve differential assessment.

Methods

We longitudinally examined the potentially independent or multiplicative effect of ASD and anxiety symptom severity on RRBs in 60 children with FXS. Anxiety was measured using the Child Behavior Checklist (CBCL), ASD severity was measured using the Childhood Autism Rating Scale (CARS), and RRBs were measured using the Repetitive Behavior Scale – Revised (RBS-R). We estimated a series of moderated regression models with anxiety and ASD symptoms at the initial assessment (Time 1) as predictors of RRBs at the outcome assessment two years later (Time 2), along with an anxiety-by-ASD interaction term to determine the potential multiplicative effect of these co-occurring conditions on RRBs.

Results

Results identified a significant interaction between ASD and anxiety symptom severity at the initial assessment that predicted elevated sensory-motor RRBs two years later. Increased sensory-motor RRBs were predicted by elevated ASD symptoms only when anxiety symptom severity was low. Likewise, increased sensory-motor RRBs were predicted by elevated anxiety symptoms only when ASD symptom severity was low. Interestingly, this relationship was isolated to Sensory-Motor RRBs, with evidence that it could also apply to total RRBs.

Conclusions

Findings suggest that ASD and anxiety exert independent and differential effects on Sensory-Motor RRBs when at high severity levels and a multiplicative effect when at moderate levels, which has important implications for early and targeted interventions.
Co-design and evaluation of an audio podcast about sustainable development goals for undergraduate nursing and midwifery students
Authors: Anderson Tara , Stark Patrick , Craig Stephanie , McMullan Johanna , Anderson Gail , Hughes Clare , Gormley Kevin , Killough Jane , McLaughlin-Borlace Nuala , Steele Laura , Lynch Sara , Sánchez-Martín Jesús , Zamora-Polo Francisco , Rodman Adam , Li Rongrong , Mitchell Gary
Published: November 2024

Background

Sustainable Development Goals (SDGs) are universally recognised targets designed to combat poverty, inequality, and climate change. However, there exists limited awareness and understanding of these goals among nursing and midwifery students. To address this knowledge gap, a co-designed audio podcast was introduced as an educational tool to enhance students’ comprehension of SDGs and their relevance to the healthcare profession.

Methods

A prospective study was conducted at Queen’s University Belfast, involving 566 first-year nursing and midwifery students. A 60-minute SDG podcast, co-designed with students and stakeholders, was made accessible within the university’s learning management system. Pre- and post-test questionnaires were administered to assess changes in students’ knowledge levels and attitudes toward SDGs. Additionally, 37 participants engaged in focus group interviews six months after listening to the podcast to explore their experiences and reflections. Quantitative data was analysed using paired t-tests and descriptive statistics, while qualitative data was analysed thematically.

Results

The podcast significantly increased students’ awareness of SDGs and their understanding of the goals’ relevance to their profession and personal lives. Post-test scores showed substantial improvements across all three sub-scales: knowledge, professional relevance, and personal relevance. Moreover, participants rated the podcast as a valuable learning resource with high acceptability, although some expressed uncertainty about replay intentions. Focus group interviews revealed three themes, including 1) More than you know’, which described how participants developed new knowledge and understanding about SDGs, 2) ‘Nurse-Midwife Nudges’, which illuminated how participants made minor changes to their behaviour and 3) ‘Fitting Format’, which highlighted how participants favoured the use of an audio podcast to learn about SDGs.

Discussion

This study demonstrates the potential of audio podcasts as an effective and engaging tool for increasing awareness and understanding of SDGs among nursing and midwifery students. The results suggest that such interventions can positively impact students’ knowledge, attitudes, and behavioural intentions regarding the SDGs. The findings also emphasise the importance of co-design in developing educational resources tailored to the specific needs and preferences of students.
Exploiting acquired vulnerability to develop novel treatments for cholangiocarcinoma
Authors: Areewong Sirayot , Suppramote Orawan , Prasopporn Sunisa , Jirawatnotai Siwanon
Published: November 2024

Cholangiocarcinoma (CCA) presents a formidable therapeutic challenge due to its extensive heterogeneity and plasticity, which inevitably lead to acquired resistance to current treatments. However, recent evidence suggests that acquired drug resistance is associated with a fitness cost resulting from the myriad of acquired alterations under the selective pressure of the primary treatment. Consequently, CCA patients with acquired resistance are more susceptible to alternative therapies that are ineffective as monotherapies. This phenomenon, termed “acquired vulnerability,” has garnered significant interest in drug development, as the acquired alterations could potentially be exploited therapeutically. This review elucidates the modes of acquired vulnerability, methods for identifying and exploiting acquired vulnerabilities in cancer (particularly in CCA), and strategies to enhance the clinical efficacy of drug combinations by leveraging the principle of acquired vulnerability. Identifying acquired vulnerabilities may pave the way for novel drug combinations to effectively treat highly heterogeneous and adaptable malignancies such as CCA.
Duration of onset, body temperature and C-reactive protein can be used to predict the results of pus culture in children with acute osteomyelitis of long bones
Authors: Jia Haiting , Liu Yanan , Liu Tao
Published: November 2024

Background

With the application of PCR testing and Metagenomic Next-Generation Sequencing(mNGS), the detection rate of causative organisms in paediatric bone and joint infections has been greatly improved. The aim of our study is to identify some indicators that could be used to distinguish the culture results to optimize the use of PCR and mNGS.

Methods

In this study, a total of 117 cases of acute osteomyelitis of long bones in children who underwent pus culture were included. Patients were grouped as culture-negative (n:21) and culture-positive (n:96) groups according to the results of pus culture. Age, sex, duration of onset, maximum body temperature at onset, inflammatory indicators and D-dimer after admission were systematically collected for all patients and were compared for both groups. ROC curve (ROC) was used to evaluate the diagnostic efficiency of culture-negative. Logistic regression analysis was conducted to determine independent risk factors for culture-negative.

Results

There was no significant difference in age, sex and erythrocyte sedimentation rate between culture-negative group and culture-positive group (P > 0.05). The duration of onset was longer, and the temperature, white blood cells, neutrophils count, C-reactive protein and D-dimer were less elevated in culture-negative acute osteomyelitis (P < 0.05). Duration of onset, maximum body temperature at onset, white blood cell count, neutrophil count, C-reactive protein, and D-dimer have certain diagnostic efficacy in judging the efficacy of negative culture. Logistic regression analysis indicated that the duration of onset more than 6.5 days, the maximum body temperature at onset lower than 38.35℃ and C-reactive protein lower than 78.40 mg/L were independent risk factors for negative culture (P < 0.05).

Conclusions

Our study revealed that duration of onset more than 6.5 days, maximum body temperature at onset lower than 38.35℃ and C-reactive protein lower than 78.40 mg/L were independent risk factors for predicting negative culture. In children with this type of acute osteomyelitis, we recommend that the pus be tested by PCR or mNGS as a priority.
Distribution and abundance of Aedes caspius (Pallas, 1771) and Aedes vexans (Meigen, 1830) in the Po Plain (northern Italy)
Authors: Calzolari Mattia , Mosca Andrea , Montarsi Fabrizio , Grisendi Annalisa , Scremin Mara , Roberto Paolo , Tessarolo Carlotta , Defilippo Francesco , Gobbo Federica , Casalone Cristina , Lelli Davide , Albieri Alessandro
Published: November 2024

Background

Knowledge of the distribution and abundance of disease-causing mosquito vectors is fundamental for assessing the risk of disease circulation and introduction. Aedes caspius (Pallas, 1771) and Aedes vexans (Meigen, 1830) have been implicated, to different extents, in the circulation of several arthropod-borne viruses (arboviruses). These two mosquitoes are vectors of Tahyna virus in Europe and are considered potential vectors of Rift Valley fever virus, a virus not present but at risk of introduction on the continent.

Methods

In this work, we analysed abundance data collected during West Nile virus (WNV) surveillance in northern Italy (Po Plain) via 292 CO2-baited traps to evaluate the distribution and density of these two non-target mosquitoes. We modelled the distribution and abundance of these two mosquito species in the surveyed area using two distinct spatial analysis approaches (geostatistical and machine learning), which yielded congruent results.

Results

Both species are more abundant close to the Po River than elsewhere, but Ae. caspius is present in the eastern and western parts of the plain, linked with the occurrence of rice fields and wetlands, while Ae. vexans is observed in the middle area of the plain.

Conclusions

Presence and abundance data at the municipality level were obtained and made available through this work. This work demonstrates the importance of maintaining and improving entomological surveillance programs with an adequate sampling effort.

Graphical Abstract
Compatibility and antimicrobial activity of silver nanoparticles synthesized using Lycopersicon esculentum peels
Authors: Ali Esraa , Abu-Hussien Samah H. , Hesham Esraa , Ahmed Shimaa , Mostafa Habiba , Gamal Ahmed , El-Sayed Salwa M. , Hemdan Bahaa , Bakry Ashraf , Ebeed Naglaa M. , Elhariry Hesham , Galal Ahmed , Abd-Elhalim Basma T.
Published: November 2024

Nanoparticles have gained worldwide attention as a new alternative to chemical control agents due to their special physiochemical properties. The current study focused on the environmentally friendly synthesis of silver nanoparticles (AgNPs) using Lycopersicon esculentum peel. In addition to studying the intrinsic cytotoxic effectiveness of Le-AgNPs contribute to their antibacterial, and antifungal activities and the effect of nanoparticles on the integrity of their morphological behavior. The initiative biosynthesis of L. esculentum silver nanoparticles (Le-AgNPs) was indicated by the color change of L. esculentum (Le) extract mixed with silver nitrate (AgNO3) solution from faint pink to faint brown. UV–visible spectroscopy, Dynamic light scattering (DLS), Fourier-transform infrared spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and X-ray diffraction techniques were used to characterize biosynthesized Le-AgNPs. Results of UV–visible spectroscopy recorded surface plasmon resonance at 310 nm for SPR of 2.5. The DLS results showed particles of 186 nm with a polydispersity index of 0.573. The FTIR spectrum indicated the existence of carboxyl, hydroxyl, phenolic, and amide functional groups. The HR-TEM analysis revealed quasi-spherical crystal particles of Le-AgNPs. Le-AgNPs had a negative zeta potential of − 68.44 mV, indicating high stability. Bacillus subtilis ATCC 6633 and Escherichia coli ATCC 8739 were the most susceptible pathogens to Le-AgNPs inhibition, with inhibition zone diameters (IZDs) of 4.0 and 0.92 cm, respectively. However, Listeria monocytogenes NC 013768 and Shigella sonnei DSM 5570 were the most resistant pathogens, with IZDs of 0.92 and 0.90 cm, respectively. Le-AgNPs demonstrated good inhibitory potential against pathogenic fungi, with IZDs of 3.0 and 0.92 cm against Alternaria solani ATCC 62102 and Candida albicans DSM 1386, respectively. The cytotoxicity effect was observed at a half-maximal inhibitory concentration (IC50) of 200.53 μg/ml on human colon NCM460D normal cells.
Best practices for germline variant and DNA methylation analysis of second- and third-generation sequencing data
Authors: Bonfiglio Ferdinando , Legati Andrea , Lasorsa Vito Alessandro , Palombo Flavia , De Riso Giulia , Isidori Federica , Russo Silvia , Furini Simone , Merla Giuseppe , Coppedè Fabio , Tartaglia Marco ,   Omics Sciences - Bioinformatics and Epigenetics Working Groups of the Italian Society of Human Genetics (SIGU), Bruselles Alessandro , Pippucci Tommaso , Ciolfi Andrea , Pinelli Michele , Capasso Mario
Published: November 2024

This comprehensive review provides insights and suggested strategies for the analysis of germline variants using second- and third-generation sequencing technologies (SGS and TGS). It addresses the critical stages of data processing, starting from alignment and preprocessing to quality control, variant calling, and the removal of artifacts. The document emphasized the importance of meticulous data handling, highlighting advanced methodologies for annotating variants and identifying structural variations and methylated DNA sites. Special attention is given to the inspection of problematic variants, a step that is crucial for ensuring the accuracy of the analysis, particularly in clinical settings where genetic diagnostics can inform patient care. Additionally, the document covers the use of various bioinformatics tools and software that enhance the precision and reliability of these analyses. It outlines best practices for the annotation of variants, including considerations for problematic genetic alterations such as those in the human leukocyte antigen region, runs of homozygosity, and mitochondrial DNA alterations. The document also explores the complexities associated with identifying structural variants and copy number variations, underscoring the challenges posed by these large-scale genomic alterations. The objective is to offer a comprehensive framework for researchers and clinicians, ensuring that genetic analyses conducted with SGS and TGS are both accurate and reproducible. By following these best practices, the document aims to increase the diagnostic accuracy for hereditary diseases, facilitating early diagnosis, prevention, and personalized treatment strategies. This review serves as a valuable resource for both novices and experts in the field, providing insights into the latest advancements and methodologies in genetic analysis. It also aims to encourage the adoption of these practices in diverse research and clinical contexts, promoting consistency and reliability across studies.
Catalytic hairpin assembly-triggered endonuclease-mediated cascade signal recycling for sensitive and colorimetric miRNA detection
Authors: Xiong Xinkui , Chen Chen , Zang Bao
Published: November 2024

The catalytic hairpin assembly (CHA)-based miRNA detection methods have garnered significant attention due to their simplicity and acceptable amplification efficiency. However, these methods necessitate improved sensitivity. In this work, we present a colorimetric and ultrasensitive approach for the detection of cancer-related miRNAs which is initiated by CHA-mediated nicking endonuclease-assisted signals recycling. The target initiates the CHA process to expose the functional section in the P2 probe. This section can activate cascaded recycling cycles to produce numerous linker sequences by Nt.AlwI endonuclease-assisted cleavage of two hairpin signal probes. The 3,3’,5,5’-tetramethylbenzidine sulfate (TMB)/H2O2-based color reaction is induced by the fixation of the cDNA-HRP on the surface of magnetic beads, which is mediated by the linker sequence. The proposed method demonstrates a sensitivity that is either comparable to or superior to that of previous colorimetric miRNA detection methods, as a result of this design. Furthermore, the method demonstrated a promising potential for clinical applications and a high selectivity to target miRNA. Consequently, it provides a colorimetric assay that is both ultrasensitive and dependable for the visual detection of miRNA, which has the potential to revolutionize the early diagnosis of cancer.
Diagnostic and prognostic value of plasma lipocalin-2 levels in patients with metabolic dysfunction–associated steatotic liver disease
Authors: ElGhandour Ahmed Mohamed , Teama Nahla Mohamed , Kamal Marwa Abdullah , Nashaat Ehab Hassan , Ghani Amani Mohamed Abdel , Abdo Ahmad Abbas
Published: November 2024

Background

Non-Alcoholic Fatty Liver Disease, recently better recognised as Metabolic Dysfunction–Associated Steatotic Liver Disease, is the most prevalent form of chronic liver disease at present time. It is estimated to impact 32% of the world's population, hence representing a significant health burden.

Aim of the work

To assess the significance of plasma Lipocalin-2 (LCN2) levels in the diagnosis and prognosis of NAFLD patients.

Patients and methods

In this retrospective case–control study we recruited 102 subjects aged between 18 and 70 years. The included participants were split into two study groups. Group I: 51 NAFLD patients (61% men, 39% females) and Group II: 51 healthy controls (51% men and 49% females), for whom plasma LCN2 levels were assessed and correlated with NAFLD fibrosis score, FIB4 and fatty liver index.

Results

In this study, LCN2 levels in NAFLD patients were significantly greater compared to individuals in the control group (p < 0.001), with a mean of 1893.214 ± 1002.852 ng/dL in the cases and a mean of 466.020 ± 397.699 ng/dL in the controls. This suggests the use of LCN2 as a possible diagnostic marker of NAFLD. The mean LCN2 levels in this study also significantly increased as the grade of fatty liver increased from I to III (p < 0.001). This in turn proposes the use of LCN2 as a prognostic marker for NAFLD progression. LCN2 also significantly correlated with the fatty liver index and NAFLD Fibrosis scoring systems, but not with Fib-4. With an area under the ROC of 0.906, it demonstrated excellent diagnostic performance with 84% sensitivity, 90% specificity, 89.6% PPV and 85.2% NPV for the prediction of NAFLD patients.

Conclusion

Lipocalin-2 performs as a diagnostic and a possible prognostic marker for metabolic dysfunction-associated steatotic liver disease.
G9a promotes muscular atrophy in chronic aging and acute denervation
Authors: Jin Ying , Kang Wei , Ji Xiaoge , Zhou Yihao , Zheng Ling
Published: November 2024

Muscular atrophy accompanied by neuromuscular junction (NMJ) denervation is often observed after long-term chronic diseases and aging and is associated with substantial morbidity and mortality. Here, we report that histone methyltransferase G9a is elevated in the muscle of muscular atrophy model mice and that muscle-specific deficiency of G9a (Ehmt2Ckmm−KO) alleviates muscular atrophy in both aged and denervated mice. Moreover, increased nerve-to-myofiber ratios and increased Agrin-Lrp4-MuSK signaling, which maintains NMJ, are found in aged Ehmt2Ckmm−KO mice. Together, these data suggest that G9a promotes muscular atrophy and disrupts NMJ; thus, inhibiting the G9a level may be a potential therapy for muscular atrophy.
Integral equation-based wellbore preconditioner for 3D electromagnetic response modeling
Authors: Elliott Marcus S. , Everett Mark E.
Published: November 2024

Modeling subsurface controlled-source electromagnetic (CSEM) responses using the finite-element (FE) method is challenging in the presence of highly conductive wellbore casing. The very large conductivity contrast between the casing and the host formation leads to increased computation time and potentially unstable solutions. We address this difficulty by preconditioning an FE solver with an integral equation (IE) primary solution that captures the CSEM response of a realistic-sized steel wellbore casing. Our hybrid IE-FE approach determines the primary field using 2D IE forward modeling and then interpolates the IE-computed solution onto the nodes. Then, using an existing FE simulator, we solve for the secondary electric and magnetic fields. This approach removes the need for an ultrafine FE mesh around the wellbore, thereby improving the FE solution stability while greatly reducing the FE computation time. Our method is illustrated by modeling the CSEM responses of idealized fluid-bearing zones.
Mental disorders may prevent, not cause, suicide
Authors: Swanepoel Annie , Soper C. A.
Published: November 2024

We challenge a prevalent belief that depression causes suicide and propose that certain symptoms of depression and other psychopathologies may function to prevent lethal self-injury. Theoretical and empirical evidence supports this position. As suicide posed an extreme fitness hazard throughout human evolution, our species evolved special-purpose psychological defences that continuously monitor and manage this danger. Last-ditch protections may present as diverse psychiatric phenomena. Mobilising in adolescence and adulthood in response to chronic distress, these usually stop suicidal thoughts from escalating into deadly actions. The theory is testable. We point to important implications for the clinical management of suicide and psychopathology.
Suicide-related internet use of mental health patients: what clinicians know
Authors: Bojanić Lana , Kenworthy Jessica , Moon Tamara , Turnbull Pauline , Ibrahim Saied , Kapur Navneet , Appleby Louis , Hunt Isabelle M. , Flynn Sandra
Published: November 2024

Background

Suicide-related internet use (SRIU), defined as internet use related to one's own feelings of suicide, can be both a risk and protective factor, especially for isolated individuals. Despite its influence on suicidality, clinicians face challenges in assessing SRIU because of the private nature of internet usage. Current recommendations on enquiring about SRIU in a clinical setting concern mostly young people.

Aims

To address the gap in understanding SRIU among patients of all ages, this study aims to explore mental health clinicians’ experiences, attitudes and beliefs regarding enquiring about SRIU, as well as the risks and benefits it presents in the assessment and management of patients. Finally, the study aims to establish the role SRIU potentially plays in the assessment and management of patients.

Method

Twelve clinicians practising at secondary mental health services in England participated in interviews. Thematic analyses were used for data interpretation.

Results

Clinicians who participated in interviews rarely initiate discussions on SRIU with their patients despite considering this an important factor in suicidality. Age of both patients and clinicians has the potential to influence enquiry into SRIU. Clinicians recognise the potential benefits of patients finding supportive online communities but also express concerns about harmful and low-quality online content related to suicide.

Conclusions

Integrating SRIU enquiry into standard clinical practice, regardless of the patient's age, is an important step towards comprehensive patient care. Broader training for clinicians on enquiring about online behaviours is essential to mitigate potential risks and harness the benefits of SRIU in mental health patients.
Feminism and the Cinema of Experience
Authors: Marso Lori Jo
Published: November 2024

From popular films like Greta Gerwig’s Barbie (2023) to Chantal Akerman’s avant-garde classic Jeanne Dielman (1975), feminist cinema can provoke discomfort. Ambivalence, stasis, horror, cringe—these and other affects refuse the resolution of feeling good or bad, leaving viewers questioning and disoriented. In Feminism and the Cinema of Experience, Lori Jo Marso examines how filmmakers scramble our senses to open up space for encountering and examining the political conditions of patriarchy, racism, and existential anxiety. Building on Akerman’s cinematic lexicon and Simone de Beauvoir’s phenomenological attention to the lives of girls and women, Marso analyzes film and television by directors ranging from Akerman, Gerwig, Mati Diop, Catherine Breillat, and Joey Soloway to Emerald Fennell, Michaela Coel, Audrey Diwan, Alice Diop, and Julia Ducournau. Through their innovative and intentional uses of camera, sound, editing, and new forms of narrative, these directors use discomfort in order to invite viewers to feel like feminists and to sense the possibility of freedom.
Influence of acupuncture and acupressure on labor duration
Authors: Meneses Neves Nathália , Augustus de Oliveira Silva Leonardo , Aguilar de Oliveira Isabela , Annunciato Biondi Lucca , Pitta Costa Luz Roberta
Published: November 2024

Objective: in this regard, the main goal of this systematic review is to identify and compare results that can build different perspectives on the efficacy of acupuncture using work time. Methods: the model followed for the construction of this systematic review was the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The keywords chosen were "Acupuncture" and "Labor Time". Studies originally published were analyzed in English and Portuguese between 1987 and 2020. The selected articles were those including acupuncture and acupressure techniques during labor. Results: 17 articles were in eligibility criteria. In six studies, the use of a standard questionnaire was not described. Sixteen were randomized clinical trials. Fourteen articles had the size of the sample equal to or less than one hundred, and only two exceeded three hundred participants. In most studies (n = 12), the early average acupuncture was between 37 ° and 42 ° week of pregnancy. Ten studies used acupuncture as the main technique. All cited the types of points used, including more present: SP6 and LI4. Conclusion: Acupuncture can be considered an effective technique in reducing the duration of work. However, more high-quality articles are needed to consolidate these positive results.
Maternal and perinatal death surveillance and response in Bangladesh: A case study on measuring impact through health information systems
Authors: Sayem Abu Sadat Mohammad , Kaasbøll Jens Johan , Halim Abul , Abdullah Dr. Abu Sayeed Md.
Published: November 2024

The health sector in Bangladesh is thriving, and the Maternal and Perinatal Death Surveillance and Response (MPDSR) program has been in place to investigate the causes of maternal and perinatal death. This study aimed to investigate the availability of information systems for measuring the status of an MPDSR program and the planning process and actions developed based on real-time information. This study delved into innovative information systems that can be used to monitor and measure the impact of health programs. The study included both the primary and secondary data. A total of six key informant interviews were conducted to explore the enablers and strengths of information systems. Secondary data were collected from government websites and MPDSR action plans. This study highlights the potential and challenges of governmental health information systems in notifying maternal and neonatal deaths. While the current notification rates are not up to national standards, it is encouraging to see health managers develop action plans and address this issue. The MPDSR information system is a valuable tool to help health managers measure changes in maternal and perinatal mortality rates and design locally targeted interventions. However, several obstacles must be addressed, such as insufficient data, lack of monitoring, and irregularities in MPDSR review meetings. By finding ways to overcome these challenges, we can unlock the full potential of MPDSR and improve maternal and neonatal health outcomes. The success of an MPDSR program relies on how the information is utilized to take action and measure the outcome. Advanced information systems, such as health dashboards, scorecards, and administrative data, can play a vital role in measuring the progress and impact of a program. The primary challenge lies in capturing all deaths, including their causes, and having the capacity to analyze the data effectively to develop action plans for health managers.
Unveiling Efficiency: A Research Inquiry into Technical Staff Utilization in South Indian Clinical Laboratory using Workload Indicators Staffing Need (WSN) Analysis.
Authors: Thomas Shalet , G Somu , Belurkar Sushma , Bari Tarushree , Patil Asha
Published: November 2024

Background Effective clinical laboratories are the need of the hour, they play a significant role in healthcare, providing tests for diagnosis, treatment monitoring. Having skilled and adequate staffing is crucial for clinical laboratories to operate efficiently, manage their workload effectively, and deliver test results in a timely manner. Despite being critical for healthcare delivery, ensuring adequate staffing levels in clinical laboratories remains a complex challenge for healthcare providers worldwide due to resource constraints and workforce shortages. Methods This study evaluates the effectiveness of clinical laboratory staff utilization by considering workload, work pressure, and staffing requirements. We utilized the Workload Indicators of Staffing Need (WISN) method to assess the staffing in the clinical laboratory of a tertiary care hospital. Annual hospital statistics were collected for two years from June 2021 to May 2023 to calculate the average number of days (234) worked annually, percentage of workload, distribution of activity time measurement units, and the ratio of needed, surplus, and existing staff. The findings aim to provide valuable insights for optimizing staffing levels and ensuring an efficient laboratory environment. Result The analysis found a significant workforce gap in the laboratory between the current staff numbers and the calculated staffing needs. The WISN ratios ranged from 0.2 to 0.7, indicating the existing 33 staff members face a high workload burden. The calculated ideal staffing level was 46.33 personnel. These results demonstrate staff shortage and excess workload pressure on the current laboratory employees. Conclusion The results highlight the importance of optimizing staffing levels in clinical laboratories to ensure quality service delivery. The WISN methodology can be a useful tool in healthcare facilities for making evidence -based decisions for staff allocation, maximizing the utilization of employee skill sets, and establishing standard staffing benchmarks tailored to the needs of clinical laboratories.
Types of refractive errors in a sample of Iraqi children with Intermittent exotropia
Authors: K. Mohammad Najah , Ali Rajab Ibrahim
Published: November 2024

Background One of the most common strabismus types in children is intermittent exotropia, which masks the deviation of one eye outward, mostly in children aged 2-4 years. It may affect visual development and often coexists with refractive mistakes. Unlike esotropia, which usually links to hyperopia, intermittent exotropia might have a different relationship with refractive errors and thus lead to myopia. Methods In this cross-sectional study from August 2021 to December 2023, 179 patients diagnosed with intermittent exotropia were recruited via an outpatient clinic of Najah Al-Quraishi, Baghdad, Iraq. The refractive errors were compared by autorefractometry and retinoscopy after cycloplegic dilation. Data analysis was constructed under the use of IBM SPSS V.26 for the determination of emmetropia, myopia, and hyperopia prevalence. Results The most expected refractive error among the 179 patients was emmetropia, which occurred in 68 patients (38%). Low hyperopia and low myopia were also common, with 64 and 40, respectively. A limited number of patients had moderate/high myopia and moderate/high hyperopia; in detail, the prevalence was following: 5% of patients had moderate myopia, while 0.5% of patients suffered from high myopia; symmetrically, 5% were moderate to high hyperopia. From the data collected, a trend emerged for a low refractive error and symmetric refractive error in both eyes. Conclusion Contrasted to prior conventional wisdom about refractive error in strabismus, it established the greater incidence of emmetropia in patients with intermittent exotropia. The findings call for specific management strategies to be applied in this population.