bluuebunny/crossref_metadata_2025 huggingface dataset:
BIM to BEM for Building Energy Analysis: A Review of Interoperability Strategies
Authors: Ciccozzi Annamaria , de Rubeis Tullio , Paoletti Domenica , Ambrosini Dario
Published: November 2023

The main objective of this review is to summarize and thoroughly investigate the most popular and promising BIM (building information modeling) and BEM (building energy modeling) interoperability strategies employed in the last years (2004–2023), highlighting pros and cons of each strategy and trying to understand the reason for the still limited BIM–BEM interaction. This review summarizes the main countries, areas, modeling tools, and interoperability strategies, with the advantages and disadvantages of each one. The methodology is based on the PRISMA protocol, and two databases were used for the research: Scopus and Google Scholar. A total of 532 publications were selected and 100 papers were deemed useful for the purposes of this review. The main findings led to the identification of four different interoperability strategies between BIM and BEM tools: (1) real-time connection; (2) standardized exchange formats and middleware corrective tools; (3) adherence to model view definitions; (4) proprietary tool-chain. These strategies were found to be characterized by different degrees of complexity, time required for information exchange, proprietary or opensource software, ability to reduce information loss, and detailed energy results. The results of this study showed that, to date, there is no better interoperability strategy, and that further efforts are needed so that interoperability between the two tools can become commonplace.
Research Progress on CO2 Capture, Utilization, and Storage (CCUS) Based on Micro-Nano Fluidics Technology
Authors: Pan Xiuxiu , Sun Linghui , Huo Xu , Feng Chun , Zhang Zhirong
Published: November 2023

The research and application of CO2 storage and enhanced oil recovery (EOR) have gradually emerged in China. However, the vast unconventional oil and gas resources are stored in reservoir pores ranging from several nanometers to several hundred micrometers in size. Additionally, CO2 geological sequestration involves the migration of fluids in tight caprock and target layers, which directly alters the transport and phase behavior of reservoir fluids at different scales. Micro- and nanoscale fluidics technology, with their advantages of in situ visualization, high temperature and pressure resistance, and rapid response, have become a new technical approach to investigate gas–liquid interactions in confined domains and an effective supplement to traditional core displacement experiments. The research progress of micro–nano fluidics visualization technology in various aspects, such as CO2 capture, utilization, and storage, is summarized in this paper, and the future development trends and research directions of micro–nano fluidics technology in the field of carbon capture, utilization, and storage (CCUS) are predicted.
Hydrogen Cooling of Turbo Aggregates and the Problem of Rotor Shafts Materials Degradation Evaluation
Authors: Balitskii Alexander I. , Syrotyuk Andriy M. , Havrilyuk Maria R. , Balitska Valentina O. , Kolesnikov Valerii O. , Ivaskevych Ljubomyr M.
Published: November 2023

Changes in the properties of 38KhN3MFA steel, from which the rotor shaft is made, were investigated by comparing the hardness of the shaft surface and hydrogen concentration in the chips and analyzing changes in the morphology of the chips under the influence of various factors. The microstructures obtained from the surface of the rotor shaft samples are presented, and histograms reflecting the parameters of the structural components are constructed. An abbreviated diagram of the “life cycle” of the turbine rotor shaft is given. It was found that, during long-term operation (up to 250 thousand hours), the hardness of the rotor shaft surface decreases from 290 HB to 250 HB. It was recorded that, in the microstructure of the shaft during 250 thousand hours of operation, the amount of cementite decreased from 87% to 62%, and the proportion of free ferrite increased from 5% to 20%. The average values of ferrite microhardness decreased from 1.9 GPa to 1.5 GPa. An increase in the content of alloying elements in carbides was recorded: Cr and V—by 1.15–1.6 times; and Mo—by 2.2–2.8 times. With the help of the developed program (using computer vision methods), changes in their microrelief were detected to study photos of chips.
Comparative Study of Hydrogen Production from Organic Fraction of Municipal Solid Waste and Its Challenges: A Review
Authors: Haque Naimul , Azad Abul Kalam
Published: November 2023

The growing interest in hydrogen production arises from its higher energy density, making it an attractive option for energy storage and fuel applications. However, hydrogen production relies heavily on fossil fuels, producing substantial CO2 emissions. Meanwhile, the organic fraction of municipal solid waste (OFMSW), which constitutes a significant portion of solid waste, predominantly ends up in landfills, leading to methane emissions. Harnessing hydrogen from OFMSW offers an opportunity to offset methane emissions and promote cleaner hydrogen production compared to conventional methods. Various pretreatment methods and production techniques have been explored for hydrogen production from OFMSW, including bio-photolysis, photo-fermentation, microbial electrolysis, and dark fermentation. This study presents a comparative analysis of these methods, evaluating their efficiency, scalability, and potential challenges for hydrogen fuel production from OFMSW. By exploring these avenues, this study found the current hydrogen fuel production scenarios where OFMSW contributes a small portion due to the limited yield. Microbial electrolysis can help to improve the yield and feedstock quality. This study recommends further investigation into the advancement of sustainable hydrogen production and provides insights into overcoming the obstacles associated with this promising field.
Regional Heterogeneity Analysis of Residential Electricity Consumption in Chinese Cities: Based on Spatial Measurement Models
Authors: Sun Zhenhua , Du Lingjun , Long Houyin
Published: November 2023

The share of electricity consumption by urban and rural residents in China’s overall electricity consumption is very close to that of the tertiary sector, which has become an important driver of China’s electricity consumption growth. At the same time, due to the mismatch between China’s regional resource endowments and the level of regional development, the regional supply and demand situation for electricity varies. Therefore, it is urgent to clarify the regional differences in residential electricity consumption and the factors affecting it, and accordingly adopt targeted and feasible measures to regulate residential electricity consumption. This article includes data from 285 Chinese prefecture-level cities from 2006 to 2019, and adopts a “three lines” method of region-partitioning (Qinling–Huaihe line, Huhuanyong line, and Shanhaiguan line) to divide four regions. We used spatial econometric models to examine residential electricity consumption and its influencing factors in China from the standpoint of regional heterogeneity. The results show that there is significant regional heterogeneity in residential electricity consumption in China, and the difference between the north of the Shanhaiguan line and other areas is significant. Moreover, there is a positive spatial correlation in the per capita domestic electricity consumption of urban residents, and each influencing factor has obvious regional heterogeneity, among which household appliances are the significant influencing factor. Based on the regional heterogeneity of residential electricity consumption, management measures should be formulated according to local conditions, and the supply of electricity should be ensured by strengthening multidimensional initiatives.
Cooling Strategy Optimization of Cylindrical Lithium-Ion Battery Pack via Multi-Counter Cooling Channels
Authors: Jeon Hyeonchang , Hong Seokmoo , Yun Jinwon , Han Jaeyoung
Published: November 2023

This study focused on the design of a battery pack cooling channel based on a Tesla Model S electric car. This study aimed to achieve a balance between cooling efficiency and pressure drop while maintaining safe and optimal operating temperatures for the batteries. A cooling channel design similar to the basic type employed in the Tesla Model S using 448 cylindrical Li-ion batteries was considered. Consequently, important parameters, such as the maximum temperature and temperature difference in the battery cells in a module, as well as the pressure drop of the coolant, were analyzed. In addition, the characteristics of the temperature changes in each cooling channel shape were investigated. The temperature limit for the battery in a module and the temperature limit difference were set to 40 °C and 5 °C, respectively, to evaluate the performance of the cooling system. Further, the effects of discharge rates (3C and 5C), cooling channel shapes (counter flow and parallel types), and coolant inlet velocities (0.1, 0.2, 0.3, and 0.4 m/s) on battery thermal management were analyzed. The results revealed that the parallel type channel yielded a lower pressure drop than the basic type channel; however, it was not as effective in removing heat from the battery. In contrast, the counter flow type channel effectively removed heat from the batteries with a higher coolant pressure drop in the channel. Therefore, a multi-counter flow type cooling channel combining the advantages of both these channels was proposed to decrease the pressure drop while maintaining appropriate operating temperatures for the battery module. The proposed cooling channel exhibited an excellent cooling performance with lower power consumption and better heat transfer characteristics. However, relatively minimal differences were confirmed for the maximum temperature and temperature difference in the battery module compared with the counter flow type. Therefore, the proposed cooling channel type can be implemented to ensure the optimal temperature operation of the battery module and to decrease system power consumption.
Modulating the Acoustic Vibration Performance of Wood by Introducing a Periodic Annular Groove Structure
Authors: Zhang Liang , He Lan , Liang Yuwei , Zhang Juncheng , Zhang Haiyang , Zhou Jing , Cui Haotian , Li Mingrui , Miao Yuanyuan , Liu Zhenbo
Published: November 2023

The acoustic vibration performance of wood affects the quality of many musical instruments, and the variability of wood causes obvious differences between individual timber samples. To mitigate the variations among the individual timber samples intended for musical instruments, in this study, we combined finite element simulation with experimental testing to investigate the effect of the periodic annular groove structure on the comprehensive acoustic vibration characteristics of wood. The results revealed that there are discernible correlations between the structural parameters of the periodic annular groove and the key acoustic parameters of wood, including the resonant frequency, equivalent dynamic modulus of elasticity, equivalent specific dynamic modulus of elasticity, equivalent acoustic radiation quality constant, and equivalent acoustic impedance. These relationships can be used to fine-tune the overall acoustic vibration performance of wood and harmonize the acoustic vibration characteristics among different timber specimens. The effects of the periodic annular groove structure on the five acoustic vibration parameters obtained through finite element simulations exhibited minimal differences to the corresponding results from experimental tests. Furthermore, there was a remarkably strong correlation between the outcomes of the finite element simulations and the experimental test results, with the coefficient of determination exceeding 0.99.
Evaluation of Optical Genome Mapping in Clinical Genetic Testing of Facioscapulohumeral Muscular Dystrophy
Authors: Kovanda Anja , Lovrečić Luca , Rudolf Gorazd , Babic Bozovic Ivana , Jaklič Helena , Leonardis Lea , Peterlin Borut
Published: November 2023

Facioscapulohumeral muscular dystrophy (FSHD) is the third most common hereditary muscular dystrophy, caused by the contraction of the D4Z4 repeats on the permissive 4qA haplotype on chromosome 4, resulting in the faulty expression of the DUX4 gene. Traditional diagnostics are based on Southern blotting, a time- and effort-intensive method that can be affected by single nucleotide variants (SNV) and copy number variants (CNV), as well as by the similarity of the D4Z4 repeats located on chromosome 10. We aimed to evaluate optical genome mapping (OGM) as an alternative molecular diagnostic method for the detection of FSHD. We first performed optical genome mapping with EnFocus™ FSHD analysis using DLE-1 labeling and the Saphyr instrument in patients with inconclusive diagnostic Southern blot results, negative FSHD2 results, and clinically evident FSHD. Second, we performed OGM in parallel with the classical Southern blot analysis for our prospectively collected new FSHD cases. Finally, panel exome sequencing was performed to confirm the presence of FSHD2. In two patients with diagnostically inconclusive Southern blot results, OGM was able to identify shortened D4Z4 repeats on the permissive 4qA alleles, consistent with the clinical presentation. The results of the prospectively collected patients tested in parallel using Southern blotting and OGM showed full concordance, indicating that OGM is a useful alternative to the classical Southern blotting method for detecting FSHD1. In a patient showing clinical FSHD but no shortened D4Z4 repeats in the 4qA allele using OGM or Southern blotting, a likely pathogenic variant in SMCHD1 was detected using exome sequencing, confirming FSHD2. OGM and panel exome sequencing can be used consecutively to detect FSHD2.
Safety of Janssen Ad26.COV.S and Astra Zeneca AZD1222 COVID-19 Vaccines among Mobile Phone Users in Malawi: Findings from a National Mobile-Based Syndromic Surveillance Survey, July 2021 to December 2021
Authors: Makonokaya Lucky , Kapanda Lester , Woelk Godfrey B. , Chauma-Mwale Annie , Kalitera Louiser Upile , Nkhoma Harrid , Zimba Suzgo , Chamanga Rachel , Golowa Cathy , Machekano Rhoderick , Maphosa Thulani
Published: November 2023

The safety profiles of the Ad26.COV2.S and AZD1222 COVID-19 vaccines have not been described in the general population in Malawi. We present self-reported adverse events (AE) following the receipt of these vaccines in Malawi as part of a national syndromic surveillance survey. We conducted phone-based syndromic surveillance surveys among adults (≥18 years) with verbal consent. We used secure tablets through random digit dialing to select mobile phone numbers and collected data electronically. Survey questions included whether the respondent had received the COVID-19 vaccines, whether they had experienced any AE following vaccination, and the severity of the AE. We used multivariable analysis to identify factors associated with self-reported AE post-COVID-19 vaccination. A total of 11,924 (36.0%) out of 33,150 respondents reported receiving at least one dose of either Ad26.COV2.S or AZD1222 between July–December 2021; of those, 65.1% were female. About 49.2% of the vaccine recipients reported at least one AE, 90.6% of which were mild, and 2.6% were severe. Higher education level and concern about the safety of COVID-19 vaccines were associated with AE self-report (Adjusted Odds Ratio [AOR] 2.63 [95% CI 1.96–3.53] and 1.44, [95% CI 1.30–1.61], respectively), while male gender and older age were associated with reduced likelihood of AE self-report (AORs 0.81, [95% CI 0.75–0.88], 0.62 [95% CI 0.50–0.77], respectively). Ad26.COV2.S and AZD1222 vaccines are well-tolerated, with primarily mild and few severe AE among adults living in Malawi. Self-reporting of AE following COVID-19 vaccination is associated with gender, age, education, and concern about the safety of the vaccines. Recognizing these associations is key when designing and implementing COVID-19 vaccination communication messages to increase vaccination coverage.
Assessing the Ecotoxicity of Eight Widely Used Antibiotics on River Microbial Communities
Authors: Pino-Otín María Rosa , Lorca Guillermo , Langa Elisa , Roig Francisco , Terrado Eva M. , Ballestero Diego
Published: November 2023

Global prevalence of antibiotic residues (ABX) in rivers requires ecotoxicological impact assessment. River microbial communities serve as effective bioindicators for this purpose. We quantified the effects of eight commonly used ABXs on a freshwater river microbial community using Biolog EcoPlates™, enabling the assessment of growth and physiological profile changes. Microbial community characterization involved 16S rRNA gene sequencing. The river community structure was representative of aquatic ecosystems, with the prevalence of Cyanobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes. Our findings reveal that all ABXs at 100 µg/mL reduced microbial community growth and metabolic capacity, particularly for polymers, carbohydrates, carboxylic, and ketonic acids. Chloramphenicol, erythromycin, and gentamicin exhibited the highest toxicity, with chloramphenicol notably impairing the metabolism of all studied metabolite groups. At lower concentrations (1 µg/mL), some ABXs slightly enhanced growth and the capacity to metabolize substrates, such as carbohydrates, carboxylic, and ketonic acids, and amines, except for amoxicillin, which decreased the metabolic capacity across all metabolites. We explored potential correlations between physicochemical parameters and drug mechanisms to understand drug bioavailability. Acute toxicity effects at the river-detected low concentrations (ng/L) are unlikely. However, they may disrupt microbial communities in aquatic ecosystems. The utilization of a wide array of genetically characterized microbial communities, as opposed to a single species, enables a better understanding of the impact of ABXs on complex river ecosystems.
Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii
Authors: Budhwani Zafina , Buragina Jenna T. , Lang Jen , Acedo Jeella Z.
Published: November 2023

The rise of drug-resistant bacteria is a major threat to public health, highlighting the urgent need for new antimicrobial compounds and treatments. Bacteriocins, which are ribosomally synthesized antimicrobial peptides produced by bacteria, hold promise as alternatives to conventional antibiotics. In this study, we identified and characterized a novel leaderless bacteriocin, bawcin, the first bacteriocin to be characterized from a Bacillus wiedmannii species. Chemically synthesized and purified bawcin was shown to be active against a broad range of Gram-positive bacteria, including foodborne pathogens Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes. Stability screening revealed that bawcin is stable over a wide range of pH (2.0–10.0), temperature conditions (25–100 °C), and against the proteases, papain and pepsin. Lastly, three-dimensional structure homology modeling suggests that bawcin contains a saposin-fold with amphipathic helices and a highly cationic surface that may be critical for membrane interaction and the subsequent cell death of its targets. This study provides the foundational understanding of the activity and properties of bawcin, offering valuable insights into its applications across different antimicrobial uses, including as a natural preservative in food and livestock industries.
Genome-Wide Analysis of the Polygalacturonase Gene Family Sheds Light on the Characteristics, Evolutionary History, and Putative Function of Akebia trifoliata
Authors: Yi Xiaoxiao , Chen Wei , Guan Ju , Zhu Jun , Zhang Qiuyi , Yang Huai , Yang Hao , Zhong Shengfu , Chen Chen , Tan Feiquan , Ren Tianheng , Luo Peigao
Published: November 2023

Polygalacturonase (PG) is one of the largest families of hydrolytic enzymes in plants. It is involved in the breakdown of pectin in the plant cell wall and even contributes to peel cracks. Here, we characterize PGs and outline their expression profiles using the available reference genome and transcriptome of Akebia trifoliata. The average length and exon number of the 47 identified AktPGs, unevenly assigned on 14 chromosomes and two unassembled contigs, were 5399 bp and 7, respectively. The phylogenetic tree of 191 PGs, including 47, 57, 51, and 36 from A. trifoliata, Durio zibethinus, Actinidia chinensis, and Vitis vinifera, respectively, showed that AktPGs were distributed in all groups except group G and that 10 AktPGs in group E were older, while the remaining 37 AktPGs were younger. Evolutionarily, all AktPGs generally experienced whole-genome duplication (WGD)/segmental repeats and purifying selection. Additionally, the origin of conserved domain III was possibly associated with a histidine residue (H) substitute in motif 8. The results of both the phylogenetic tree and expression profiling indicated that five AktPGs, especially AktPG25, could be associated with the cracking process. Detailed information and data on the PG family are beneficial for further study of the postharvest biology of A. trifoliata.
SMALL PLANT AND ORGAN 1 (SPO1) Encoding a Cellulose Synthase-like Protein D4 (OsCSLD4) Is an Important Regulator for Plant Architecture and Organ Size in Rice
Authors: Qiao Lei , Wu Qilong , Yuan Liuzhen , Huang Xudong , Yang Yutao , Li Qinying , Shahzad Nida , Li Haifeng , Li Wenqiang
Published: November 2023

Plant architecture and organ size are considered as important traits in crop breeding and germplasm improvement. Although several factors affecting plant architecture and organ size have been identified in rice, the genetic and regulatory mechanisms remain to be elucidated. Here, we identified and characterized the small plant and organ 1 (spo1) mutant in rice (Oryza sativa), which exhibits narrow and rolled leaf, reductions in plant height, root length, and grain width, and other morphological defects. Map-based cloning revealed that SPO1 is allelic with OsCSLD4, a gene encoding the cellulose synthase-like protein D4, and is highly expressed in the roots at the seedling and tillering stages. Microscopic observation revealed the spo1 mutant had reduced number and width in leaf veins, smaller size of leaf bulliform cells, reduced cell length and cell area in the culm, and decreased width of epidermal cells in the outer glume of the grain. These results indicate the role of SPO1 in modulating cell division and cell expansion, which modulates plant architecture and organ size. It is showed that the contents of endogenous hormones including auxin, abscisic acid, gibberellin, and zeatin tested in the spo1 mutant were significantly altered, compared to the wild type. Furthermore, the transcriptome analysis revealed that the differentially expressed genes (DEGs) are significantly enriched in the pathways associated with plant hormone signal transduction, cell cycle progression, and cell wall formation. These results indicated that the loss of SPO1/OsCSLD4 function disrupted cell wall cellulose synthase and hormones homeostasis and signaling, thus leading to smaller plant and organ size in spo1. Taken together, we suggest the functional role of SPO1/OsCSLD4 in the control of rice plant and organ size by modulating cell division and expansion, likely through the effects of multiple hormonal pathways on cell wall formation.
MicroRNAs as Potential Biomarkers of Environmental Exposure to Polycyclic Aromatic Hydrocarbons and Their Link with Inflammation and Lung Cancer
Authors: Letelier Pablo , Saldías Rolando , Loren Pía , Riquelme Ismael , Guzmán Neftalí
Published: November 2023

Exposure to atmospheric air pollution containing volatile organic compounds such as polycyclic aromatic hydrocarbons (PAHs) has been shown to be a risk factor in the induction of lung inflammation and the initiation and progression of lung cancer. MicroRNAs (miRNAs) are small single-stranded non-coding RNA molecules of ~20–22 nucleotides that regulate different physiological processes, and their altered expression is implicated in various pathophysiological conditions. Recent studies have shown that the regulation of gene expression of miRNAs can be affected in diseases associated with outdoor air pollution, meaning they could also be useful as biomarkers of exposure to environmental pollution. In this article, we review the published evidence on miRNAs in relation to exposure to PAH pollution and discuss the possible mechanisms that may link these compounds with the expression of miRNAs.
Adhesion G Protein-Coupled Receptor G2 Promotes Hepatocellular Carcinoma Progression and Serves as a Neutrophil-Related Prognostic Biomarker
Authors: Wu Qian , Wang Pei , Peng Qihang , Kang Zhongcui , Deng Yiting , Li Jiayi , Chen Ying , Li Jin , Ge Feng
Published: November 2023

Adhesion G protein-coupled receptor G2 (ADGRG2) is an orphan adhesion G protein-coupled receptor (GPCR), which performs a tumor-promoting role in certain cancers; however, it has not been systematically investigated in hepatocellular carcinoma (HCC). In the current study, we utilized multiple databases to analyze the expression and diagnostic and prognostic value of ADGRG2 in HCC and its correlation with immune infiltration and inflammatory factors. The function and upstream regulatory miRNA of ADGRG2 were validated through qPCR, Western blot, CCK8, wound healing, and dual luciferase assays. It turned out that ADGRG2 was significantly higher in HCC and had a poor survival rate, especially in AFP ≤ 400 ng/mL subgroups. Functional enrichment analysis suggested that ADGRG2 may be involved in cancer pathways and immune-related pathways. In vitro, siRNA-mediated ADGRG2 silencing could inhibit the proliferation and migration of Huh7 and HepG2 cells. There was a highly significant positive correlation between ADGRG2 and neutrophils. Moreover, NET-related genes were filtered and confirmed, such as ENO1 and S100A9. Meanwhile, the high expression of ADGRG2 was also accompanied by the highest number of inflammatory cytokines, chemokines, and chemokine receptors and good immunotherapy efficacy. Finally, AGDGR2 may be sensitive to two drugs (PIK-93 and NPK76-II-72-1) and can be targeted by miR-326. In conclusion, ADGRG2 may serve as a novel biomarker and drug target for HCC diagnosis, immunotherapy, and prognosis and was related to neutrophils and the inflammatory process of liver cancer development.
Albumin-Based Liver Reserve Models vs. MELD 3.0 in Prognostic Prediction for Hepatocellular Carcinoma Patients with Renal Insufficiency
Authors: Ho Shu-Yein , Liu Po-Hong , Hsu Chia-Yang , Tseng Hung-Ting , Huang Yi-Hsiang , Su Chien-Wei , Hou Ming-Chih , Huo Teh-Ia
Published: November 2023

The severity of liver functional reserve is an important prognostic predictor in hepatocellular carcinoma (HCC). The albumin–bilirubin (ALBI), easy (EZ)-ALBI, platelet-albumin–bilirubin (PALBI), platelet–albumin (PAL) score, and MELD 3.0 score are used to evaluate the severity of liver dysfunction. However, their prognostic role in HCC patients, specifically with renal insufficiency (RI), is unclear. We aimed to investigate the predictive accuracy of the five models in these patients. A total of 1120 newly diagnosed HCC patients with RI were enrolled. A multivariate Cox proportional analysis was used to identify independent predictors associated with survival. In the Cox model, older age, an α-fetoprotein ≥20 ng/mL, vascular invasion, a medium and high tumor burden score, poor performance status, a higher ALBI grade, an EZ-ALBI grade, a PALBI grade, a PAL grade, and MELD 3.0 score were all independently associated with decreased overall survival (all p < 0.001). Among the five liver reserve models, the ALBI grade is the best surrogate marker to represent liver functional reserve in terms of outcome prediction. The albumin-based liver reserve models (ALBI, EZ-ALBI, PALBI, and PAL) and MELD 3.0 are all feasible prognostic markers to indicate liver injury, specifically in HCC patients with RI. Among them, the ALBI grade is the most robust tool for survival prediction in these patients.
Transcriptome Analysis of mRNA and lncRNA Related to Muscle Growth and Development in Gannan Yak and Jeryak
Authors: Wei Yali , Guo Dashan , Bai Yanbin , Liu Zhanxin , Li Jingsheng , Chen Zongchang , Shi Bingang , Zhao Zhidong , Hu Jiang , Han Xiangmin , Wang Jiqing , Liu Xiu , Li Shaobin , Zhao Fangfang
Published: November 2023

The production performance of Jeryak, resulting from the F1 generation of the cross between Gannan yak and Jersey cattle, exhibits a significantly superior outcome compared with that of Gannan yak. Therefore, we used an RNA-seq approach to identify differentially expressed mRNAs (DEMs) and differentially expressed lncRNAs (DELs) influencing muscle growth and development in Gannan yaks and Jeryaks. A total of 304 differentially expressed lncRNAs and 1819 differentially expressed mRNAs were identified based on the screening criteria of |log 2 FC| > 1 and FDR < 0.05. Among these, 132 lncRNAs and 1081 mRNAs were found to be down-regulated, while 172 lncRNAs and 738 mRNAs were up-regulated. GO and KEGG analyses showed that the identified DELs and DEMs were enriched in the entries of pathways associated with muscle growth and development. On this basis, we constructed an lncRNA–mRNA interaction network. Interestingly, two candidate DELs (MSTRG.16260.9 and MSTRG.22127.1) had targeting relationships with 16 (MYC, IGFBP5, IGFBP2, MYH4, FGF6, etc.) genes related to muscle growth and development. These results could provide a basis for further studies on the roles of lncRNAs and mRNAs in muscle growth in Gannan yaks and Jeryak breeds.
Changes in the Phenotype of Intramural Inhibitory Neurons of the Porcine Descending Colon Resulting from Glyphosate Administration
Authors: Bulc Michał , Całka Jarosław , Palus Katarzyna
Published: November 2023

Environmental contamination and the resulting food contamination represent a serious problem and pose a major threat to animal and human health. The gastrointestinal tract is directly exposed to a variety of substances. One is glyphosate, whose presence in the soil is commonly observed. This study demonstrates the effects of low and high glyphosate doses on the populations of intramural neurons of the porcine descending colon. An analysis was performed on neurons ex-pressing the vasoactive intestinal peptide, pituitary adenylate cyclase-activating peptide, a neuronal isoform of nitrogen oxide synthase, and galanin. Even a low dose of glyphosate increased the number of neurons immunoreactive against the studied substances. However, the changes depended on both the plexus analysed and the substance tested. Meanwhile, a high glyphosate dose resulted in quantitative changes (an increase in the number) within neurons immunoreactive against all the studied neuropeptides/enzymes in the myenteric plexus and both submucosal plexuses. The response of the enteric nervous system in the form of an increase in the number of neurons immunoreactive against neuroprotective substances may suggest that glyphosate has a toxic effect on enteric neurons which attempt to increase their survivability through the released neuroprotective substances.
Rare A360T Mutation Alters GSK3β(Ser9) Binding in the Cytosolic Loop of Presenilin 1, Influencing β-Catenin Nuclear Localization and Pro-Death Gene Expression in Alzheimer’s Disease Case
Authors: Wężyk Michalina , Berdyński Mariusz , Figarski Adam , Skrzypczak Magdalena , Ginalski Krzysztof , Zboch Marzena , Winkel Izabela , Żekanowski Cezary
Published: November 2023

Presenilin 1 (PS1) forms, via its large cytosolic loop, a trimeric complex with N-cadherin and β-catenin, which is a key component of Wnt signaling. PS1 undergoes phosphorylation at 353 and 357 serines upon enhanced activity and elevated levels of the GSK3β isoform. PS1 mutations surrounding these serines may alter the stability of the β-catenin complex. Such mutations are found in some cases of familial early-onset Alzheimer’s disease (fEOAD), but their functional impact remains obscure. One of such variants of PS1, the A360T substitution, is located close to GSK3β-targeted serine residues. This variant was recently demonstrated in the French population, but more detail is needed to understand its biological effects. To assess the significance of this variant, we employed functional studies using a fibroblast cell line from an Alzheimer’s disease case (a female proband) carrying the A360T mutation. Based on functional transcriptomic, cellular, and biochemical assays, we demonstrated atypically impaired β-catenin/GSK3β signaling in the A360T patient’s fibroblasts. In detail, this was characterized by a decreased level of active cytosolic β-catenin and bound by PS1, an increased level of nuclear β-catenin, an increased level of inhibited GSK3β phosphorylated on Ser9, and enhanced interaction of GSK3β(Ser9) with PS1. Based on the transcriptomic profile of the A360T fibroblasts, we proposed a dysregulated transcriptional activity of β-catenin, exemplified by increased expression of various cyclin-dependent kinases and cyclins, such as cyclin D1, potentially inducing neurons’ cell cycle re-entry followed by apoptosis. The A360T cells did not exhibit significant amyloid pathology. Therefore, cell death in this PS1 cytosolic loop mutation may be attributed to impaired β-catenin/GSK3β signaling rather than amyloid deposition per se. We further estimated the biological and clinical relevance of the A360T variant by whole exome sequencing (WES). WES was performed on DNA from the blood of an A360T female proband, as well as an unrelated male patient carrying the A360T mutation and his mutation-free daughter (both unavailable for the derivation of the fibroblast cell lines). WES confirmed the highest-priority AD causality of the A360T variant in PS1 and also profiled the pathways and processes involved in the A360T case, highlighting the greatest importance of altered Wnt signaling.
Studies with Human-Induced Pluripotent Stem Cells Reveal That CTNS Mutations Can Alter Renal Proximal Tubule Differentiation
Authors: Thiyagarajan Ramkumar , Taub Mary
Published: November 2023

Cystinosis is an autosomal recessive disease resulting from mutations in ctns, which encodes for cystinosin, a proton-coupled cystine transporter that exports cystine from lysosomes. The major clinical form, infantile cystinosis, is associated with renal failure due to the malfunctioning of the renal proximal tubule (RPT). To examine the hypothesis that the malfunctioning of the cystinotic RPT arises from defective differentiation, human-induced pluripotent stem cells (hiPSCs) were generated from human dermal fibroblasts from an individual with infantile cystinosis, as well as a normal individual. The results indicate that both the cystinotic and normal hiPSCs are pluripotent and can form embryoid bodies (EBs) with the three primordial germ layers. When the normal hiPSCs were subjected to a differentiation regime that induces RPT formation, organoids containing tubules with lumens emerged that expressed distinctive RPT proteins, including villin, the Na+/H+ Exchanger (NHE) isoform 3 (NHE3), and the NHE Regulatory Factor 1 (NHERF1). The formation of tubules with lumens was less pronounced in organoids derived from cystinotic hiPSCs, although the organoids expressed villin, NHE3, and NHERF1. These observations can be attributed to an impairment in differentiation and/or by other defects which cause cystinotic RPTs to have an increased propensity to undergo apoptosis or other types of programmed cell death.
Mechanisms That Protect Mammalian Sperm from the Spontaneous Acrosome Reaction
Authors: Breitbart Haim , Grinshtein Elina
Published: November 2023

To acquire the capacity to fertilize the oocyte, mammalian spermatozoa must undergo a series of biochemical reactions in the female reproductive tract, which are collectively called capacitation. The capacitated spermatozoa subsequently interact with the oocyte zona-pellucida and undergo the acrosome reaction, which enables the penetration of the oocyte and subsequent fertilization. However, the spontaneous acrosome reaction (sAR) can occur prematurely in the sperm before reaching the oocyte cumulus oophorus, thereby jeopardizing fertilization. One of the main processes in capacitation involves actin polymerization, and the resulting F-actin is subsequently dispersed prior to the acrosome reaction. Several biochemical reactions that occur during sperm capacitation, including actin polymerization, protect sperm from sAR. In the present review, we describe the protective mechanisms that regulate sperm capacitation and prevent sAR.
Col1A-2 Mutation in Osteogenesis Imperfecta Mice Contributes to Long Bone Fragility by Modifying Cell-Matrix Organization
Authors: André Grégoire , Chretien Antoine , Demoulin Antoine , Beersaerts Mélanie , Docquier Pierre-Louis , Behets Catherine
Published: November 2023

Osteogenesis imperfecta (OI) is a rare congenital bone dysplasia generally caused by a mutation of one of the type I collagen genes and characterized by low bone mass, numerous fractures, and bone deformities. The collagen organization and osteocyte lacuna arrangement were investigated in the long bones of 17-week-old wildtype (WT, n = 17) and osteogenesis imperfecta mice (OIM, n = 16) that is a validated model of severe human OI in order to assess their possible role in bone fragility. Fractures were counted after in vivo scanning at weeks 5, 11, and 17. Humerus, femur, and tibia diaphyses from both groups were analyzed ex vivo with pQCT, polarized and ordinary light histology, and Nano-CT. The fractures observed in the OIM were more numerous in the humerus and femur than in the tibia, whereas the quantitative bone parameters were altered in different ways among these bones. Collagen fiber organization appeared disrupted, with a lower birefringence in OIM than WT bones, whereas the osteocyte lacunae were more numerous, more spherical, and not aligned in a lamellar pattern. These modifications, which are typical of immature and less mechanically competent bone, attest to the reciprocal alteration of collagen matrix and osteocyte lacuna organization in the OIM, thereby contributing to bone fragility.
The Role of the Cumulative Illness Rating Scale (CIRS) in Estimating the Impact of Comorbidities on Chronic Obstructive Pulmonary Disease (COPD) Outcomes: A Pilot Study of the MACH (Multidimensional Approach for COPD and High Complexity) Study
Authors: Di Raimondo Domenico , Pirera Edoardo , Pintus Chiara , De Rosa Riccardo , Profita Martina , Musiari Gaia , Siscaro Gherardo , Tuttolomondo Antonino
Published: November 2023

Background. Chronic obstructive pulmonary disease (COPD) is a heterogeneous systemic syndrome that often coexists with multiple comorbidities. In highly complex COPD patients, the role of the Cumulative Illness Rating Scale (CIRS) as a risk predictor of COPD exacerbation is not known. Objective. The objective of this study was determine the effectiveness of the CIRS score in detecting the association of comorbidities and disease severity with the risk of acute exacerbations in COPD patients. Methods. In total, 105 adults with COPD (mean age 72.1 ± 9.0 years) were included in this prospective study. All participants at baseline had at least two moderate exacerbations or one leading to hospitalization. The primary outcome was a composite of moderate or severe COPD exacerbation during the 12 months of follow-up. Results. The CIRS indices (CIRS total score, Severity Index and Comorbidity Index) showed a positive correlation with modified Medical Research Council (mMRC), COPD assessment test (CAT) and a negative correlation with forced expiratory volume in the first second (FEV1), Forced Vital Capacity (FVC), and FEV1/FVC. The three CIRS indices were able to predict the 12-month rate of moderate or severe exacerbation (CIRS Total Score: Hazard Ratio (HR) = 1.12 (95% CI: 1.08–1.21); CIRS Severity Index: HR = 1.21 (95% CI: 1.12–1.31); CIRS Comorbidity Index = 1.58 (95% CI: 1.33–1.89)). Conclusions. Among patients with COPD, the comorbidity number and severity, as assessed by the CIRS score, influence the risk in moderate-to-severe exacerbations. The CIRS score also correlates with the severity of respiratory symptoms and lung function.
The Effect of Changes in the Aging Temperature Combined with Deep Cryogenic Treatment on the Structure, Phase Composition, and Micromechanical Properties of the WE43 Magnesium Alloy
Authors: Barylski Adrian , Aniołek Krzysztof , Dercz Grzegorz , Matuła Izabela , Rak Jan , Mazur Izabela
Published: November 2023

This paper examines the optimal aging temperature of WE43 alloy that has undergone precipitation hardening in conjunction with deep cryogenic treatment. The microstructure and phase composition were investigated, a microanalysis of the chemical composition was performed, and instrumental indentation tests were performed to determine the parameters of the micro-mechanical properties of the alloy after different heat treatment variants. It has been proven that a decrease in the aging temperature from 250 °C to 225 °C and the introduction of a deep cryogenic treatment lead to favorable changes in the microstructure of the alloy (reduction in grain size, increase in the number, and change in the type of β-phase precipitates). The changes in the alloy structure achieved by lowering the aging temperature contribute to the improvement of the micromechanical properties of the test material. The most advantageous results were recorded for an alloy subjected to solution treatment and aged at 225 °C for 24 h with deep cryogenic treatment: a 30% increase in hardness, a 10% increase in Young’s modulus, an improvement in elastic properties, and increased resistance to deformation of the alloy were shown compared to the initial (as-received) state. Raising the aging temperature to 250 °C leads to a phenomenon known as alloy overaging for both alloys after classical precipitation hardening and after deep cryogenic treatment. The results indicate the significant effectiveness of the proposed heat treatment in improving the service life of the Mg-Y-Nd-Zr (WE43) alloy.
Enhanced Anti-Corrosion Performance of Co-Cr-Mo Alloy in Molten Al by Prior Oxidation Treatment
Authors: Shang Rongrong , Yang Biaobiao , Li Yunping
Published: November 2023

Co-based alloys are promising alternatives to replace the currently used tool steels in aluminum die-casting molds for producing sophisticated products. Although the reaction is significantly less severe compared to that of tool steels, bare Co-29Cr-6Mo (CCM) alloy is still gradually corroded under molten Al, leading to the local failure of the alloy due to the formation of intermetallic compounds between the matrix and molten Al. This study indicated that prior oxidation treatment at 750 °C on CCM alloy is beneficial in enhancing the corrosion resistance of the alloy to molten Al. The is more pronounced in the alloy after a longer oxidation treatment. However, after oxidation for longer than 24 h, the protectiveness of the film cannot be enhanced anymore. In addition, even after the full failure of the oxide film, the thickness loss rate of a sample with prior oxidation treatment is much lower than that of a bare sample. This can be attributed to the fact that network-aligned oxide particles of the cone structure boundary inhibit both the outwards movements of alloying elements and the dissolution of the intermetallic layer.
Synthesis and Characterization of Bi4Ti3O12 Nanoparticles Obtained via Pulsed Laser Ablation in Liquids
Authors: García-Quiñonez Linda Viviana , Colorado-Garrido Dario , Collado-Hernández Adolfo , Acuña-Leal Daniel Arturo , Castillo-Rodríguez Guadalupe Alan , Fernández-González Daniel , Martínez-Guerra Eduardo , Mendívil-Palma María Isabel , Gomez-Rodríguez Cristian
Published: November 2023

Bismuth titanate (BTO) nanoparticles were obtained by pulsed laser ablation in liquid media (PLAL). Distilled water, ethanol, isopropanol, and acetone were used as media for laser ablation experiments, in which the colloidal solutions were obtained. Laser ablation was carried out using the second harmonic and fundamental wavelength of a pulsed Nd:YAG laser (532 nm and 1064 nm, respectively) with laser fluences of 25 and 12 mJ/cm2, respectively. Transmission electron microscopy was utilized for morphological characterization. BTO nanoparticles obtained have spherical shapes with orthorhombic structure and the average size distribution depended on the liquid media nature. In alcohols, BTO NPs were spherical with a carbon layer around them. X-ray diffraction, UV-Vis absorption spectra, and X-ray photoelectron spectroscopy were used to confirm the structural, optical, and elemental properties of the ablated products. The presented results show that PLAL is a viable technique for the synthesis of high-quality BTO nanoparticles with enhanced optical properties for possible applications in photocatalysis.
Synthesis of a Stable and High-Concentration BaHfxTi1−xO3 Sol–Gel for High Electromechanical Performance of Bulk Ceramics
Authors: Brault Damien , Richardot Thomas , Boy Philippe , Belleville Philippe , Levassort Franck , Bavencoffe Maxime
Published: November 2023

Lead-based materials are widely used in piezoceramics due to their high electromechanical properties. However, due to environmental protection and sustainable development, the use of the toxic element lead (Pb) in electronic devices is strictly restricted, therefore requiring the rapid development of piezoelectric-based devices with lead-free ceramics. In this context, a lead-free doped barium titanate was studied with a dual objective. First, a new sol–gel method to synthesize Hf4+-doped BaHfxTi1−xO (BHT) with x = 0.05, 0.075, and 0.10 is presented. Such BHT sols were prepared at high concentrations of up to 1 M. Dilution in ethylene glycol allowed parameters (viscosity, colloid sizes, etc.) to be controlled, which ensured a time-stable sol for several months at room temperature. Second, densified bulk ceramics with attrited powders were obtained from these sols and showed very good electromechanical properties, with a thickness coupling factor of kt = 47% (BaHf0.05Ti0.95O3 sintered at 1500 °C/6 h). These results are a first step that will allow the processing of lead-free piezoelectric thick films using a sol–gel composite method for vibrational energy harvesting applications.
Effect of Duty Cycle on Cutting Force for Ultrasonic Vibration-Assisted Milling Carbon Fiber-Reinforced Polymer Laminates
Authors: Zhang Yukun , Ren Junxue , Zhou Jinhua
Published: November 2023

Cutting force is an important factor that affects the surface quality of machining carbon fiber-reinforced polymer (CFRP). High cutting force can lead to surface damage such as the burrs and the delamination in the machining process of CFRP. Ultrasonic vibration-assisted machining (UVAM) can reduce the cutting force in the machining process. This work is focused on the relationship between the duty cycle and the cutting force in UVAM of CFRP. Based on the kinematics of UVAM, the movement of the cutting tool edge and the tool–workpiece separation in UVAM were analyzed, and a calculation formula for the duty cycle was obtained. The milling experiment of CFRP was conducted to compare the cutting force between UVAM and conventional machining (CM), and the relationship between the reduction in the cutting force in UVAM and the duty cycle was determined. The experimental results showed that when the duty cycle was 0.2916, the cutting force of UVAM was reduced by 7.4% to 27% compared with that of CM. When the duty cycle was 1, the cutting force of UVAM was reduced by −4.5% to 7.5% compared with that of CM. Therefore, the effect of reducing the cutting force of UVAM can be enhanced by adjusting the process parameters to reduce the duty cycle of UVAM, and a lower cutting force can be obtained.