bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Vegetation Succession on Bearing Capacity of Forest Roads Made of Unbound Aggregates
Authors: Grajewski Sylwester M. , Czerniak Andrzej , Kurowska Ewa E.
Published: October 2020

The aim of the research was to verify a common opinion concerning a positive influence of plants on the bearing capacity and durability of forest roads made of unbound aggregates. The surface bearing capacity is defined as the ability to transfer traffic loads without any excessive deformations which would hinder regular use of the surface and shorten its durability. It is a significant functional feature of any road. The article analyzed the influence of road surface plant succession on its bearing parameters. The research was conducted on sections of experimental road constructed using macadam technology and reinforced partly with a biaxial geogrid. Measurements were taken with a lightweight Zorn ZFG 3000 GPS type deflectometer with a 300 mm pressure plate radius and 10 kg drop weight which allowed to measure dynamic deformation modulus (Evd) and s/v parameter regarded as an indicator of compaction accuracy of the studied layer. Evd values and s/v parameters, which were obtained by measuring the road pavement covered in vegetation and after having it mechanically removed (mowed), were submitted to the analysis; next, they were compared with the results of an analysis done on areas naturally deprived of the plant cover and located in the immediate vicinity of the measuring points. The conducted research has indicated unfavorable influence of vegetation succession on the bearing parameters of the analyzed sections. The greatest drop in the mean Evd value was 39%, and s/v parameter deteriorated as much as 9%. Hence, a regular mowing of the road surface (including the maneuvering, storage and passing areas) should be taken as standard and mandatory procedures of forest road maintenance.
Temperature Reduction in Urban Surface Materials through Tree Shading Depends on Surface Type Not Tree Species
Authors: T.U.N. Kaluarachichi , M.G. Tjoelker , S. Pfautsch
Published: October 2020

Trees play a vital role in urban cooling. The present study tested if key canopy characteristics related to tree shade could be used to predict the cooling potential across a range of urban surface materials. During the austral summer of 2018–2019, tree and canopy characteristics of 471 free-standing trees from 13 species were recorded across Greater Sydney, Australia. Stem girth and tree height, as well as leaf area index and ground-projected crown area was measured for every tree. Surface temperatures were recorded between noon (daylight saving time) and 3:00 p.m. under the canopy of each tree in the shade and in full sun to calculate the temperature differential between adjacent sunlit and shaded surfaces (∆Ts). The limited control over environmental parameters was addressed by using a large number of randomly selected trees and measurement points of surface temperatures. Analyses revealed that no systematic relationship existed among canopy characteristics and ∆Ts for any surface material. However, highly significant differences (p < 0.001) in ∆Ts existed among surface materials. The largest cooling potential of tree shade was found by shading bark mulch (∆Ts = −24.8 °C ± 7.1), followed by bare soil (∆Ts = −22.1 °C ± 5.5), bitumen (∆Ts = −20.9 °C ± 5.8), grass (∆Ts = −18.5 °C ± 4.8) and concrete pavers (∆Ts = −17.5 °C ± 6.0). The results indicate that surface material, but not the tree species, matters for shade cooling of common urban surfaces. Shading bark mulch, bare soil or bitumen will provide the largest reductions in surface temperature, which in turn results in effective mitigation of radiant heat. This refined understanding of the capacity of trees to reduce thermal loads in urban space can increase the effectiveness of urban cooling strategies.
A Knowledge-Driven Multimedia Retrieval System Based on Semantics and Deep Features
Authors: Rinaldi Antonio Maria , Russo Cristiano , Tommasino Cristian
Published: October 2020

In recent years the information user needs have been changed due to the heterogeneity of web contents which increasingly involve in multimedia contents. Although modern search engines provide visual queries, it is not easy to find systems that allow searching from a particular domain of interest and that perform such search by combining text and visual queries. Different approaches have been proposed during years and in the semantic research field many authors proposed techniques based on ontologies. On the other hand, in the context of image retrieval systems techniques based on deep learning have obtained excellent results. In this paper we presented novel approaches for image semantic retrieval and a possible combination for multimedia document analysis. Several results have been presented to show the performance of our approach compared with literature baselines.
Terrestrial Laser Scanner for the Formal Assessment of a Roman-Medieval Structure—The Cloister of the Cathedral of Tarragona (Spain)
Authors: Buill Felipe , Núñez-Andrés M. Amparo , Costa-Jover Agustí , Moreno David , Puche Josep M. , Macias Josep M.
Published: October 2020

The use of massive data capture techniques in architectural heritage has enhanced the development of new methodologies that have an important impact on their conservation and understanding. The research proposes the study of formal anomalies in the cloister of the Cathedral of Tarragona (c. 12th century). It is a relevant Gothic construction in Catalonia, with the special singularity that part of its structure is raised over an important pre-existing Roman wall. The investigation is based on a point cloud obtained with a terrestrial laser scanner (TLS) and the systematization of the 3D analysis methodology of the point cloud through different reference shapes. In general terms, the construction is in good condition, so the discrepancies between real construction and the shapes of reference are small, with some exceptions. Nevertheless, the different approximations used allowed us to identify some relevant features, such as the variability of the form of rib vaults, and the influence of the criteria used in the definition and location of reference forms.
Ionophore Toxin Maduramicin Produces Haff Disease-Like Rhabdomyolysis in a Mouse Model
Authors: Gao Xiuge , Song Xinhao , Zuo Runan , Yang Dan , Ji Chunlei , Ji Hui , Peng Lin , Qiu Yawei , Guo Dawei , Jiang Shanxiang
Published: October 2020

Maduramicin is a toxic ionophore antibiotic that is isolated from Streptomyces, frequently occurring in an aquatic environment. To understand the potential role of maduramicin in crayfish consumption related Haff disease, a mouse model was established in this study. Two exposure routes of maduramicin in the abdominal muscle and the hepatopancreas tissue homogenates of crayfish were given intragastrically to mice in different doses for seven days. Action changes, clinical symptoms, feed consumption, body weight, blood biochemistry, and histopathology examination of mice were observed and analyzed. In the natural exposure group, relatively low concentration of maduramicin in crayfish muscle and hepatopancreas had no obvious effects on mental state, body weight, blood biochemical indexes, or histologic appearance. However, in the artificial exposure group, with increasing concentrations, maduramicin in crayfish muscle and hepatopancreas homogenates both induced mental sluggishness and weight loss of mice. Blood biochemical examination showed that 3.5 mg·kg−1 and 7 mg·kg−1 maduramicin in crayfish tissue homogenates significantly increased levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), and creatine kinase (CK). Additionally, histopathological examination showed that multiple organs were damaged by maduramicin, including degeneration of liver cells, shedding of renal epithelial cells, and disturbance and partial lysis of myocardial and skeletal muscle filaments in the mice. In summary, maduramicin may not cause Haff disease through contamination of the aquatic environment under normal conditions. Maduramicin can be used as a potential toxin tool to establish a rhabdomyolysis disease animal model for drug development.
Heat, Heatwaves and Cardiorespiratory Hospital Admissions in Helsinki, Finland
Authors: Sohail Hasan , Kollanus Virpi , Tiittanen Pekka , Schneider Alexandra , Lanki Timo
Published: October 2020

Background: There is a lack of knowledge concerning the effects of ambient heat exposure on morbidity in Northern Europe. Therefore, this study aimed to evaluate the relationships of daily summertime temperature and heatwaves with cardiorespiratory hospital admissions in the Helsinki metropolitan area, Finland. Methods: Time series models adjusted for potential confounders, such as air pollution, were used to investigate the associations of daily temperature and heatwaves with cause-specific cardiorespiratory hospital admissions during summer months of 2001–2017. Daily number of hospitalizations was obtained from the national hospital discharge register and weather information from the Finnish Meteorological Institute. Results: Increased daily temperature was associated with a decreased risk of total respiratory hospital admissions and asthma. Heatwave days were associated with 20.5% (95% CI: 6.9, 35.9) increased risk of pneumonia admissions and during long or intense heatwaves also with total respiratory admissions in the oldest age group (≥75 years). There were also suggestive positive associations between heatwave days and admissions due to myocardial infarction and cerebrovascular diseases. In contrast, risk of arrhythmia admissions decreased 20.8% (95% CI: 8.0, 31.8) during heatwaves. Conclusions: Heatwaves, rather than single hot days, are a health threat affecting morbidity even in a Northern climate.
Youth Resilience to Drought: Learning from a Group of South African Adolescents
Authors: Theron Linda , Ruth Mampane Motlalepule , Ebersöhn Liesel , Hart Angie
Published: October 2020

Exposure to drought is on the increase, also in sub-Saharan Africa. Even so, little attention has been paid to what supports youth resilience to the stressors associated with drought. In response, this article reports a secondary analysis of qualitative data generated in a phenomenological study with 25 South African adolescents (average age 15.6; majority Sepedi-speaking) from a drought-impacted and structurally disadvantaged community. The thematic findings show the importance of personal, relational, and structural resources that fit with youths’ sociocultural context. Essentially, proactive collaboration between adolescents and their social ecologies is necessary to co-advance socially just responses to the challenges associated with drought.
Overweight and Obesity in Children of Immigrant Versus Native Parents: Exploring a Local Setting in Portugal
Authors: Moreira Susana , Gonçalves Luzia
Published: October 2020

In Portugal, the majority of immigrants come from Portuguese-speaking countries. Among children, overweight and obesity are serious public health concerns, but a few studies include children with immigrant background. This study aimed to estimate the prevalence of overweight and obesity and risk of abdominal obesity in school-age children and to explore potential determinants, considering the origin of the children’s parents (both mother and father are non-Portuguese, only one Portuguese, and both are Portuguese). A cross-sectional study included a random sample of 789 children (5–11 years old, 49.2% of males and 50.8% female) attending public primary schools in Barreiro, district of Setubal, Portugal. Fieldwork occurred from 20th April to 3rd July 2009. Data concerning socioeconomic, eating habits, and sports outside school were obtained through a questionnaire applied to the children’s person in charge. Anthropometric measures were collected by certified technicians. One-third of the children presented overweight and obesity (33.7%, 95% CI [30.0; 37.7]) and risk of abdominal obesity (16.4%, 95% CI [13.6; 19.7]) without differences according to parents’ origin. Children with immigrant background presented higher consumption of low-priced, high-sugar, and high-fat foods, with a worse situation for girls. Females from both non-Portuguese parents also practiced less sports outside school than those where one or two parents are Portuguese. Thus, promoting and monitoring a healthy diet and physical activity in this group should be prioritized in this local setting.
An Experimental Investigation into Promoting Mental Health Service Use on Social Media: Effects of Source and Comments
Authors: Niu Zhaomeng , Hu Lun , Jeong David C. , Brickman Jared , Stapleton Jerod L.
Published: October 2020

Mental health is an increasingly prevalent topic of public interest, but remains a complex area requiring focused research that must account for negative perceptions surrounding mental health issues. The current work explores the roles of social media information source credibility and valence of social media comments on health outcomes in such a mental health context. We used a 2 (message source: professional vs. layperson) × 3 (valence of comments: positive vs. negative vs. mixed) online experiment to examine the effects of source and valence of comments on trust, attitudes and intentions related to mental health information and services among 422 undergraduate students. Results supported the hypothesized model in which source influenced cognitive trust while comments influenced affective trust. Cognitive and affective trust both impacted attitudes towards mental health information which encourages the intention to share such information on social media. Additionally, affective trust impacted attitudes towards mental services which influenced intentions to seek them out. Source and valence of comments on social media impact different behavioral intentions regarding the use of mental health services. This study provides insights for future social media campaigns promoting mental health service use.
The Effects of Resistance Training on Blood Pressure in Preadolescents and Adolescents: A Systematic Review and Meta-Analysis
Authors: Guillem Carles Miguel , Loaiza-Betancur Andrés Felipe , Rebullido Tamara Rial , Faigenbaum Avery D. , Chulvi-Medrano Iván
Published: October 2020

The aim was to systematically review and meta-analyze the current evidence for the effects of resistance training (RT) on blood pressure (BP) as the main outcome and body mass index (BMI) in children and adolescents. Two authors systematically searched the PubMed, SPORTDiscus, Web of Science Core Collection and EMBASE electronic databases. Inclusion criteria were: (1) children and adolescents (aged 8 to 18 years); (2) intervention studies including RT and (3) outcome measures of BP and BMI. The selected studies were analyzed using the Cochrane Risk-of-Bias Tool. Eight articles met inclusion criteria totaling 571 participants. The mean age ranged from 9.3 to 15.9 years and the mean BMI of 29.34 (7.24) kg/m2). Meta-analysis indicated that RT reduced BMI significantly (mean difference (MD): −0.43 kg/m2 (95% CI: −0.82, −0.03), P = 0.03; I2 = 5%) and a non-significant decrease in systolic BP (SBP) (MD: −1.09 mmHg (95% CI: −3.24, 1.07), P = 0.32; I2 = 67%) and diastolic BP (DBP) (MD: −0.93 mmHg (95% CI: −2.05, 0.19), P = 0.10; I2 = 37%). Limited evidence suggests that RT has no adverse effects on BP and may positively affect BP in youths. More high-quality studies are needed to clarify the association between RT and BP in light of body composition changes throughout childhood and adolescence.
Maternal Dietary Patterns during Pregnancy and Their Association with Gestational Weight Gain and Nutrient Adequacy
Authors: Cano-Ibáñez Naomi , Martínez-Galiano Juan Miguel , Luque-Fernández Miguel Angel , Martín-Peláez Sandra , Bueno-Cavanillas Aurora , Delgado-Rodríguez Miguel
Published: October 2020

Several epidemiologic studies have shown an association between Gestational Weight Gain (GWG) and offspring complications. The GWG is directly linked to maternal dietary intake and women’s nutritional status during pregnancy. The aim of this study was (1) to assess, in a sample of Spanish pregnant women, the association between maternal dietary patterns and GWG and (2) to assess maternal dietary patterns and nutrient adequate intake according to GWG. A retrospective study was conducted in a sample of 503 adult pregnant women in five hospitals in Eastern Andalusia (Spain). Data on demographic characteristics, anthropometric values, and dietary intake were collected from clinical records by trained midwives. Usual food intake was gathered through a validated Food Frequency Questionnaire (FFQ), and dietary patterns were obtained by principal component analysis. Nutrient adequacy was defined according to European dietary intake recommendations for pregnant women. Regression models adjusted by confounding factors were constructed to study the association between maternal dietary pattern and GWG, and maternal dietary patterns and nutritional adequacy. A negative association was found between GWG and the Mediterranean dietary pattern (crude β = −0.06, 95% CI: −0.11, −0.04). Independent of maternal dietary pattern, nutrient adequacy of dietary fiber, vitamin B9, D, E, and iodine was related to a Mediterranean dietary pattern (p < 0.05). A Mediterranean dietary pattern is related to lower GWG and better nutrient adequacy. The promotion of healthy dietary behavior consistent with the general advice promoted by the Mediterranean Diet (based on legumes, vegetables, nuts, olive oil, and whole cereals) will offer healthful, sustainable, and practical strategies to control GWG and ensure adequate nutrient intake during pregnancy.
“Kept in Check”: Representations and Feelings of Social and Health Professionals Facing Intimate Partner Violence (IPV)
Authors: Di Napoli Immacolata , Carnevale Stefania , Esposito Ciro , Block Roberta , Arcidiacono Caterina , Procentese Fortuna
Published: October 2020

Social and health professionals facing gender-based violence in Intimate Partner Violence (IPV) express feelings and thoughts closely connected to their place of work and the users of their services. However, research on professionals’ reflexivity and their implications has not been closely investigated. Therefore, this article will describe representations of IPV among social and health professionals facing gender-based violence as well as their personal feelings in accomplishing their job. Fifty interviews with health and social professionals were analyzed using grounded theory methodology supported by Atlas.ti 8.4. Five macrocategories will describe this phenomenon, leading to the final explicative core category that summarizes professionals’ attitudes toward it. Being “kept in check” among partners, partners and families, services, and institutional duties is the core category that best expressed their feelings. Therefore, implications for services and training will be further discussed.
Perceived Risk and Intentions to Practice Health Protective Behaviors in a Mining-Impacted Region
Authors: Cooper Courtney M. , Langman Jeff B. , Sarathchandra Dilshani , Vella Chantal A. , Wardropper Chloe B.
Published: October 2020

Effective risk communication strategies are critical to reducing lead exposure in mining-impacted communities. Understanding the strength of the associations between perceived risk and individuals’ behavioral intentions to protect their health is important for developing these strategies. We conducted a survey within three communities of northern Idaho, USA (n = 306) in or near a Superfund Megasite with legacy mining contamination. Survey data were used to test a theoretical model based on the Health Belief Model. Respondents had higher intentions to practice health protective behaviors when they perceived the risk of lead contamination as severe and recognized the benefits of practicing health protective behaviors. Women reported higher behavioral intentions than men, but age and mining affiliation were not significantly associated with behavioral intentions. Although managing lead hazards in communities impacted by mining is challenging due to widely distributed contamination, effective health risk messages, paired with remediation, are powerful tools to protect the health and safety of residents.
The Effect of Smoking during Pregnancy on Severity and Directionality of Externalizing and Internalizing Symptoms: A Genetically Informed Approach
Authors: Ekblad Mikael O. , Marceau Kristine , Rolan Emily , Palmer Rohan H. C. , Todorov Alexandre , Heath Andrew C. , Knopik Valerie S.
Published: October 2020

The objective was to examine the association between maternal smoking during pregnancy (SDP) and (I) severity and (II) directionality of externalizing and internalizing symptoms in a sample of sibling pairs while rigorously controlling for familial confounds. The Missouri Mothers and Their Children Study is a family study (N = 173 families) with sibling pairs (aged 7 to 16 years) who are discordant for exposure to SDP. This sibling comparison study is designed to disentangle the effects of SDP from familial confounds. An SDP severity score was created for each child using a combination of SDP indicators (timing, duration, and amount). Principal component analysis of externalizing and internalizing behavior, assessed with the Child Behavior Checklist and Teacher Report Form, was used to create symptom severity and directionality scores. The variance in severity and directionality scores was primarily a function of differences between siblings (71% and 85%, respectively) rather than differences across families (29% and 15%, respectively). The severity score that combines externalizing and internalizing symptom severity was not associated with SDP. However, a significant within-family effect of SDP on symptom directionality (b = 0.07, p = 0.04) was observed in the sibling comparison model. The positive directionality score indicates that SDP is associated with differentiation of symptoms towards externalizing rather than internalizing symptoms after controlling for familial confounds with a sibling comparison model. This supports a potentially causal relationship between SDP and externalizing behavior.
Generalized Hyperbolic Distribution and Portfolio Efficiency in Energy and Stock Markets of BRIC Countries
Authors: Núñez-Mora José Antonio , Sánchez-Ruenes Eduardo
Published: October 2020

Oil, also called black gold, is considered as the commodity which has the greatest impact on the world’s economy, and it has been studied in terms of its relationship and effects on macroeconomic variables such as Gross Domestic Product (GDP), inflation, trade balance, exchange rate and some others. Likewise, the relationship of oil with the financial market has been deepened and is very interesting in the case of emergent economies such as Brazil, Russia, India and China (BRIC) countries. There are many studies and approaches to this topic, but few of them focus on seeking investment opportunities through the diversification of these variables and therefore creating efficient portfolios using other distribution from the norm. This research proposes the construction of diversified portfolios with the returns of the indexes and oil mixes of the BRIC countries modeled under a Normal Inverse Gaussian (NIG) distribution, which is a notable member of the Generalized Hyperbolic (GH) family, and analyzing the effect on investment, by the inclusion of each variable into the portfolio. An important property of the GH family is that the correlations matrix of the returns is obtained from estimation of the parameters of empirical distribution through maximum likelihood. The results show in an optimal configuration, that each instrument of India, China and Brazil, contributes to the portfolio efficiency, in contrast to the index and oil mix of Russia, that do not contribute significantly.
Urban Water Demand Simulation in Residential and Non-Residential Buildings Based on a CityGML Data Model
Authors: Bao Keyu , Padsala Rushikesh , Thrän Daniela , Schröter Bastian
Published: October 2020

Humans’ activities in urban areas put a strain on local water resources. This paper introduces a method to accurately simulate the stress urban water demand in Germany puts on local resources on a single-building level, and scalable to regional levels without loss of detail. The method integrates building geometry, building physics, census, socio-economy and meteorological information to provide a general approach to assessing water demands that also overcome obstacles on data aggregation and processing imposed by data privacy guidelines. Three German counties were used as validation cases to prove the feasibility of the presented approach: on average, per capita water demand and aggregated water demand deviates by less than 7% from real demand data. Scenarios applied to a case region Ludwigsburg in Germany, which takes the increment of water price, aging of the population and the climate change into account, show that the residential water demand has the change of −2%, +7% and −0.4% respectively. The industrial water demand increases by 46% due to the development of economy indicated by GDP per capita. The rise of precipitation and temperature raise the water demand in non-residential buildings (excluding industry) of 1%.
Detecting Destroyed Communities in Remote Areas with Personal Electronic Device Data: A Case Study of the 2017 Puebla Earthquake
Authors: Marx Andrew , Poynor Mia , Kim Young-Kyung , Oberreiter Lauren
Published: October 2020

Large-scale humanitarian disasters often disproportionately damage poor communities. This effect is compounded when communities are remote with limited connectivity and response is slow. While humanitarian response organizations are increasingly using a wide range of satellites to detect damaged areas, these images can be delayed days or weeks and may not tell the story of how many or where people are affected. In order to address the need of identifying severely damaged communities due to humanitarian disasters, we present an algorithmic approach to leverage pseudonymization locational data collected from personal cell phones to detect the depopulation of localities severely affected by the 2017 Puebla earthquake in Mexico. This algorithm capitalizes on building a pattern of life for these localities, first establishing which pseudonymous IDs are a resident of the locality and then establishing what percent of those residents leave those localities after the earthquake. Using a study of 15 localities severely damaged and 15 control localities unaffected by the earthquake, this approach successfully identified 73% of severely damaged localities. This individual-focused system provides a promising approach for organizations to understand the size and severity of a humanitarian disaster, detect which localities are most severely damaged, and aid them in prioritizing response and reconstruction efforts.
Virus–Host Protein–Protein Interactions between Human Papillomavirus 16 E6 A1 and D2/D3 Sub-Lineages: Variances and Similarities
Authors: Dayer Guillem , Masoom Mehran L. , Togtema Melissa , Zehbe Ingeborg
Published: October 2020

High-risk strains of human papillomavirus are causative agents for cervical and other mucosal cancers, with type 16 being the most frequent. Compared to the European Prototype (EP; A1), the Asian-American (AA; D2/D3) sub-lineage seems to have increased abilities to promote carcinogenesis. Here, we studied protein–protein interactions (PPIs) between host proteins and sub-lineages of the key transforming E6 protein. We transduced human keratinocyte with EP or AA E6 genes and co-immunoprecipitated E6 proteins along with interacting cellular proteins to detect virus–host binding partners. AAE6 and EPE6 may have unique PPIs with host cellular proteins, conferring gain or loss of function and resulting in varied abilities to promote carcinogenesis. Using liquid chromatography-mass spectrometry and stringent interactor selection criteria based on the number of peptides, we identified 25 candidates: 6 unique to AAE6 and EPE6, along with 13 E6 targets common to both. A novel approach based on pathway selection discovered 171 target proteins: 90 unique AAE6 and 61 unique EPE6 along with 20 common E6 targets. Interpretations were made using databases, such as UniProt, BioGRID, and Reactome. Detected E6 targets were differentially implicated in important hallmarks of cancer: deregulating Notch signaling, energetics and hypoxia, DNA replication and repair, and immune response.
Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections
Authors: Avendaño Catalina , Patarroyo Manuel Alfonso
Published: October 2020

The World Health Organisation (WHO) has placed twenty diseases into a group known as neglected tropical diseases (NTDs), twelve of them being parasitic diseases: Chagas’ disease, cysticercosis/taeniasis, echinococcosis, food-borne trematodiasis, human African trypanosomiasis (sleeping sickness), leishmaniasis, lymphatic filariasis, onchocerciasis (river blindness), schistosomiasis, soil-transmitted helminthiasis (ascariasis, hookworm, trichuriasis), guinea-worm and scabies. Such diseases affect millions of people in developing countries where one of the main problems concerning the control of these diseases is diagnosis-based due to the most affected areas usually being far from laboratories having suitable infrastructure and/or being equipped with sophisticated equipment. Advances have been made during the last two decades regarding standardising and introducing techniques enabling diagnoses to be made in remote places, i.e., the loop-mediated isothermal amplification (LAMP) technique. This technique’s advantages include being able to perform it using simple equipment, diagnosis made directly in the field, low cost of each test and the technique’s high specificity. Using this technique could thus contribute toward neglected parasite infection (NPI) control and eradication programmes. This review describes the advances made to date regarding LAMP tests, as it has been found that even though several studies have been conducted concerning most NPI, information is scarce for others.
Growth and Photosynthetic Activity of Selected Spelt Varieties (Triticum aestivum ssp. spelta L.) Cultivated under Drought Conditions with Different Endophytic Core Microbiomes
Authors: Ratajczak Karolina , Sulewska Hanna , Błaszczyk Lidia , Basińska-Barczak Aneta , Mikołajczak Katarzyna , Salamon Sylwia , Szymańska Grażyna , Dryjański Leszek
Published: October 2020

The role of the microbiome in the root zone is critically important for plants. However, the mechanism by which plants can adapt to environmental constraints, especially water deficit, has not been fully investigated to date, while the endophytic core microbiome of the roots of spelt (Triticum aestivum ssp. spelta L.) grown under drought conditions has received little attention. In this study, we hypothesize that differences in the endophytic core of spelt and common wheat root microbiomes can explain the variations in the growth and photosynthetic activity of those plants, especially under drought conditions. Our greenhouse experimental design was completely randomized in a 2 × 4 × 3 factorial scheme: two water regime levels (well-watered and drought), three spelt varieties (T. aestivum ssp. spelta L.: ‘Badenstern’, ‘Badenkrone’ and ‘Zollernspelz’ and one wheat variety: T. aestivum ssp. vulgare L: ‘Dakotana’) and three mycorrhizal levels (autoclaved soil inoculation with Rhizophagus irregularis, control (autoclaved soil) and natural inoculation (non-autoclaved soil—microorganisms from the field). During the imposed stress period, relative water content (RWC), leaf chlorophyll fluorescence, gas exchange and water use efficiency (WUE) were measured. Microscopic observations of the root surface through fungi isolation and identification were conducted. Our results indicate that ‘Badenstern’ was the most drought tolerant variety, followed by ‘Zollernspelz’ and ‘Badenkrone,’ while the common wheat variety ‘Dakotana’ was the most drought sensitive. Inoculation of ‘Badenstern’ with the mycorrhizal fungi R. irregularis contributed to better growth performance as evidenced by increased whole plant and stalk dry matter accumulation, as well as greater root length and volume. Inoculation of ‘Zollernspelz’ with arbuscular mycorrhizal fungi (AMF) enhanced the photochemical efficiency of Photosystem II and significantly improved root growth under drought conditions, which was confirmed by enhanced aboveground biomass, root dry weight and length. This study provides evidence that AMF have the potential to be beneficial for plant growth and dry matter accumulation in spelt varieties grown under drought conditions.
Transcriptomic Changes in Endothelial Cells Triggered by Na,K-ATPase Inhibition: A Search for Upstream Na+i/K+i Sensitive Genes
Authors: Klimanova Elizaveta A. , Sidorenko Svetlana V. , Abramicheva Polina A. , Tverskoi Artem M. , Orlov Sergei N. , Lopina Olga D.
Published: October 2020

Stimulus-dependent elevation of intracellular Ca2+ affects gene expression via well-documented calmodulin-mediated signaling pathways. Recently, we found that the addition of extra- and intracellular Ca2+ chelators increased, rather than decreased, the number of genes expressed, and that this is affected by the elevation of [Na+]i/[K+]i-ratio. This assumes the existence of a novel Na+i/K+i-mediated Ca2+i-independent mechanism of excitation-transcription coupling. To identify upstream Na+i/K+i-sensitive genes, we examined the kinetics of transcriptomic changes in human umbilical vein endothelial cells (HUVEC) subjected to Na,K-ATPase inhibition by ouabain or K+-free medium. According to our data, microRNAs, transcription factors, and proteins involved in immune response and inflammation might be considered as key components of Na+i/K+i-mediated excitation-transcription coupling. Special attention was focused on the FOS gene and the possible mechanism of transcription regulation via G-quadruplexes, non-canonical secondary structures of nucleic acids, whose stability depends on [Na+]i/[K+]i-ratio. Verification of the [Na+]i/[K+]i-sensitive transcription regulation mechanism should be continued in forthcoming studies.
Are Metal Ions That Make up Orthodontic Alloys Cytotoxic, and Do They Induce Oxidative Stress in a Yeast Cell Model?
Authors: Kovač Vito , Poljšak Borut , Primožič Jasmina , Jamnik Polona
Published: October 2020

Compositions of stainless steel, nickel-titanium, cobalt-chromium and β-titanium orthodontic alloys were simulated with mixtures of Fe, Ni, Cr, Co, Ti and Mo metal ions as potential oxidative stress-triggering agents. Wild-type yeast Saccharomyces cerevisiae and two mutants ΔSod1 and ΔCtt1 were used as model organisms to assess the cytotoxicity and oxidative stress occurrence. Metal mixtures at concentrations of 1, 10, 100 and 1000 µM were prepared out of metal chlorides and used to treat yeast cells for 24 h. Every simulated orthodontic alloy at 1000 µM was cytotoxic, and, in the case of cobalt-chromium alloy, even 100 µM was cytotoxic. Reactive oxygen species and oxidative damage were detected for stainless steel and both cobalt-chromium alloys at 1000 µM in wild-type yeast and 100 µM in the ΔSod1 and ΔCtt1 mutants. Simulated nickel-titanium and β-titanium alloy did not induce oxidative stress in any of the tested strains.
Twisted Intramolecular Charge Transfer State of a “Push-Pull” Emitter
Authors: Lee Sebok , Jen Myungsam , Pang Yoonsoo
Published: October 2020

The excited state Raman spectra of 4-dicyanomethylene-2-methyl-6-(p-dimethylaminostyryl)-4H-pyran (DCM) in the locally-excited (LE) and the intramolecular charge transfer (ICT) states have been separately measured by time-resolved stimulated Raman spectroscopy. In a polar dimethylsulfoxide solution, the ultrafast ICT of DCM with a time constant of 1.0 ps was observed in addition to the vibrational relaxation in the ICT state of 4–7 ps. On the other hand, the energy of the ICT state of DCM becomes higher than that of the LE state in a less polar chloroform solution, where the initially-photoexcited ICT state with the LE state shows the ultrafast internal conversion to the LE state with a time constant of 300 fs. The excited-state Raman spectra of the LE and ICT state of DCM showed several major vibrational modes of DCM in the LE and ICT conformer states coexisting in the excited state. Comparing to the time-dependent density functional theory simulations and the experimental results of similar push-pull type molecules, a twisted geometry of the dimethylamino group is suggested for the structure of DCM in the S1/ICT state.
Osteoporosis: From Molecular Mechanisms to Therapies 2.0
Authors: Tang Chih-Hsin
Published: October 2020

Osteoporosis is a common skeletal disorder, occurring as a result of an imbalance between bone resorption and bone formation, with bone breakdown exceeding bone building. Bone resorption inhibitors, e.g., bisphosphonates, have been designed to treat osteoporosis. Teriparatide, an anabolic agent, stimulates bone formation and corrects the characteristic changes in the trabecular microarchitecture. However, these drugs are associated with significant side effects. It is therefore crucial that we continue to research the pathogenesis of osteoporosis and seek novel modes of therapy. This editorial summarizes and discusses the themes of the ten articles published in our Special Issue “Osteoporosis: From Molecular Mechanisms to Therapies 2.0”, a continuation of our 2019 Special Issue "Osteoporosis: From Molecular Mechanisms to Therapies" (https://www.mdpi.com/journal/ijms/special_issues/osteoporosis_ijms). These Special Issues detail important global scientific findings that contribute to our current understanding of osteoporosis.
The Stimulation of Neurogenesis Improves the Cognitive Status of Aging Rats Subjected to Gestational and Perinatal Deficiency of B9–12 Vitamins
Authors: Pourié Grégory , Martin Nicolas , Daval Jean-Luc , Alberto Jean-Marc , Umoret Rémy , Guéant Jean-Louis , Bossenmeyer-Pourié Carine
Published: October 2020

A deficiency in B-vitamins is known to lead to persistent developmental defects in various organs during early life. The nervous system is particularly affected with functional retardation in infants and young adults. In addition, even if in some cases no damage appears evident in the beginning of life, correlations have been shown between B-vitamin metabolism and neurodegenerative diseases. However, despite the usual treatment based on B-vitamin injections, the neurological outcomes remain poorly rescued in the majority of cases, compared with physiological functions. In this study, we explored whether a neonatal stimulation of neurogenesis could compensate atrophy of specific brain areas such as the hippocampus, in the case of B-vitamin deficiency. Using a physiological mild transient hypoxia within the first 24 h after birth, rat-pups, submitted or not to neonatal B-vitamin deficiency, were followed until 330-days-of-age for their cognitive capacities and their hippocampus status. Our results showed a gender effect since females were more affected than males by the deficiency, showing a persistent low body weight and poor cognitive performance to exit a maze. Nevertheless, the neonatal stimulation of neurogenesis with hypoxia rescued the maze performance during adulthood without modifying physiological markers, such as body weight and circulating homocysteine. Our findings were reinforced by an increase of several markers at 330-days-of-age in hypoxic animals, such as Ammon’s Horn 1hippocampus (CA1) thickness and the expression of key actors of synaptic dynamic, such as the NMDA-receptor-1 (NMDAR1) and the post-synaptic-density-95 (PSD-95). We have not focused our conclusion on the neonatal hypoxia as a putative treatment, but we have discussed that, in the case of neurologic retardation associated with a reduced B-vitamin status, stimulation of the latent neurogenesis in infants could ameliorate their quality of life during their lifespan.
The Loss of HLA-F/KIR3DS1 Ligation Is Mediated by Hemoglobin Peptides
Authors: Hò Gia-Gia T. , Hiemisch Wiebke , Pich Andreas , Behrens Georg M. N. , Blasczyk Rainer , Bade-Doeding Christina
Published: October 2020

The human leukocyte antigen (HLA)-Ib molecule, HLA-F, is known as a CD4+ T-cell protein and mediator of HIV progression. While HLA-Ia molecules do not have the chance to select and present viral peptides for immune recognition due to protein downregulation, HLA-F is upregulated. Post HIV infection, HLA-F loses the affinity to its activating receptor KIR3DS1 on NK cells leading to progression of the HIV infection. Several studies aimed to solve the question of the biophysical interface between HLA ligands and their cognate receptors. It became clear that even an invariant HLA molecule can be structurally modified by the variability of the bound peptide. We recently discovered the ability of HLA-F to select and present peptides and the HLA-F allele-specific peptide selection from the proteomic content using soluble HLA (sHLA) technology and a sophisticated MS method. We established recombinant K562 cells that express membrane-bound HLA-F*01:01, 01:03 or 01:04 complexes. While a recombinant soluble form of KIR3DS1 did not bind to the peptide-HLA-F complexes, acid elution of the peptides resulted in the presentation of HLA-F open conformers, and the binding of the soluble KIR3DS1 receptor increased. We used CD4+/HIV− and CD4+/HIV+ cells and performed an MS proteome analysis. We could detect hemoglobin as significantly upregulated in CD4+ T-cells post HIV infection. The expression of cellular hemoglobin in nonerythroid cells has been described, yet HLA-Ib presentation of hemoglobin-derived peptides is novel. Peptide sequence analysis from HLA-F allelic variants featured hemoglobin peptides as dominant and shared. The reciprocal experiment of binding hemoglobin peptide fractions to the HLA-F open conformers resulted in significantly diminished receptor recognition. These results underpin the molecular involvement of HLA-F and its designated peptide ligand in HIV immune escape.
Associations of TIMP-3 Genetic Polymorphisms with EGFR Statuses and Cancer Clinicopathologic Development in Lung Adenocarcinoma Patients
Authors: Chang Jer-Hwa , Lai Tsung-Ching , Yang Po-Jen , Shih Pei-Chun , Yang Yi-Chieh , Lee Kai-Ling , Liu Tu-Chen , Tsao Thomas Chang-Yao , Yang Shun-Fa , Chien Ming-Hsien
Published: October 2020

Lung adenocarcinoma (LADC) is a major subtype of lung cancer, particularly among populations of East Asia. The epidermal growth factor receptor (EGFR) is the most frequently mutated oncogene promoting LADC progression and can serve as a therapeutic target in LADC. The tissue inhibitor of metalloproteinases (TIMP)-3 is a major regulator of extracellular matrix turnover via targeting of matrix metalloproteinases (MMPs), and thus, plays a critical role in tumor development and progression. The purpose of this study was to investigate potential associations among TIMP-3 genetic polymorphisms, EGFR statuses, and cancer clinicopathologic development in patients with LADC. In this study, 277 LADC patients with different EGFR statuses were recruited to dissect the allelic discrimination of TIMP-3 -1296 T>C (rs9619311), TIMP3 249T>C (rs9862), and TIMP3 261C>T (rs11547635) polymorphisms using a TaqMan allelic discrimination assay. Our data showed that compared to those LADC patients with wild-type CC homozygotes of TIMP-3 rs9862, patients harboring TT homozygotes of rs9862 were at a higher risk of developing mutant EGFR (adjusted odds ratio (AOR) = 2.530; 95% confidence interval (CI): 1.230–5.205; p = 0.012), particularly the EGFR L858R point mutation (AOR = 2.975; 95% CI: 1.182–7.488; p = 0.021). Moreover, we observed that TIMP-3 TT homozygotes of rs9862 were correlated with the incidence of EGFR mutations in patients with a smoking habit (p = 0.045). Within male patients harboring a mutant EGFR, TIMP-3 rs9862 T (CT+TT) allele carriers were at higher risk of developing an advanced stage (p = 0.025) and lymph node metastasis (p = 0.043). Further analyses of clinical datasets revealed correlations of TIMP-3 expression with a favorable prognosis in patients with LADC. In conclusion, the data suggest that TIMP-3 rs9862 polymorphisms may contribute to identify subgroups of lung cancer patients at high risk for tumor progression, among carriers of LADC-bearing mutant EGFR.
Transcriptomic Analysis of Human Astrocytes In Vitro Reveals Hypoxia-Induced Mitochondrial Dysfunction, Modulation of Metabolism, and Dysregulation of the Immune Response
Authors: Allen Scott P. , Seehra Rajpinder Singh , Heath Paul R. , Hall Benjamin P. C. , Bates Jessica , Garwood Claire J. , Matuszyk Martyna M. , Wharton Stephen B. , Simpson Julie E.
Published: October 2020

Hypoxia is a feature of neurodegenerative diseases, and can both directly and indirectly impact on neuronal function through modulation of glial function. Astrocytes play a key role in regulating homeostasis within the central nervous system, and mediate hypoxia-induced changes in response to reduced oxygen availability. The current study performed a detailed characterization of hypoxia-induced changes in the transcriptomic profile of astrocytes in vitro. Human astrocytes were cultured under normoxic (5% CO2, 95% air) or hypoxic conditions (1% O2, 5% CO2, 94% N2) for 24 h, and the gene expression profile assessed by microarray analysis. In response to hypoxia 4904 genes were significantly differentially expressed (1306 upregulated and 3598 downregulated, FC ≥ 2 and p ≤ 0.05). Analysis of the significant differentially expressed transcripts identified an increase in immune response pathways, and dysregulation of signalling pathways, including HIF-1 (p = 0.002), and metabolism, including glycolysis (p = 0.006). To assess whether the hypoxia-induced metabolic gene changes observed affected metabolism at a functional level, both the glycolytic and mitochondrial flux were measured using an XF bioanalyser. In support of the transcriptomic data, under physiological conditions hypoxia significantly reduced mitochondrial respiratory flux (p = 0.0001) but increased basal glycolytic flux (p = 0.0313). However, when metabolically stressed, hypoxia reduced mitochondrial spare respiratory capacity (p = 0.0485) and both glycolytic capacity (p = 0.0001) and glycolytic reserve (p < 0.0001). In summary, the current findings detail hypoxia-induced changes in the astrocyte transcriptome in vitro, identifying potential targets for modifying the astrocyte response to reduced oxygen availability in pathological conditions associated with ischaemia/hypoxia, including manipulation of mitochondrial function, metabolism, and the immune response.