bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Electrochemical Performances of Mn3O4/Heteroatom-Doped Reduced Graphene Oxide Aerogels as an Anode for Sodium-Ion Batteries
Authors: Mahamad Yusoff Nor Fazila , Idris Nurul Hayati , Md Din Muhamad Faiz , Majid Siti Rohana , Harun Noor Aniza
Published: October 2022

Owing to their high theoretical capacity, transition-metal oxides have received a considerable amount of attention as potential anode materials in sodium-ion (Na-ion) batteries. Among them, Mn3O4 has gained interest due to the low cost of raw materials and the environmental compatibility. However, during the insertion/de-insertion process, Mn3O4 suffers from particle aggregation, poor conductivity, and low-rate capability, which, in turn, limits its practical application. To overcome these obstacles, we have successfully prepared Mn3O4 nanoparticles distributed on the nitrogen (N)-doped and nitrogen, sulphur (N,S)-doped reduced graphene oxide (rGO) aerogels, respectively. The highly crystalline Mn3O4 nanoparticles, with an average size of 15–20 nm, are homogeneously dispersed on both sides of the N-rGO and N,S-rGO aerogels. The results indicate that the N-rGO and N,S-rGO aerogels could provide an efficient ion transport channel for electrolyte ion stability in the Mn3O4 electrode. The Mn3O4/N- and Mn3O4/N,S-doped rGO aerogels exhibit outstanding electrochemical performances, with a reversible specific capacity of 374 and 281 mAh g−1, respectively, after 100 cycles, with Coulombic efficiency of almost 99%. The interconnected structure of heteroatom-doped rGO with Mn3O4 nanoparticles is believed to facilitate fast ion diffusion and electron transfer by lowering the energy barrier, which favours the complete utilisation of the active material and improvement of the structure’s stability.
Formulation and Evaluation of Niosomal Alendronate Sodium Encapsulated in Polymeric Microneedles: In Vitro Studies, Stability Study and Cytotoxicity Study
Authors: Zaid Alkilani Ahlam , Abu-Zour Hana , Alshishani Anas , Abu-Huwaij Rana , Basheer Haneen A. , Abo-Zour Hadeel
Published: October 2022

The aim of this study is to design and evaluate a transdermal delivery system for alendronate sodium (ALS) loaded with nanocarrier to improve its permeability and prolong its release. This is due to its low bioavailability, potential gastrointestinal side effects, and the special administration needed for the oral dosage form of ALS. When using the ether injection method, various niosomal formulations were produced. Size of the particles, polydispersity index (PDI), surface charge (ZP), drug entrapment efficiency (EE), and in vitro release were used to characterize the resulting niosomes. The size of niosomes ranged between 99.6 ± 0.9 and 464.3 ± 67.6 nm, and ZP was from −27.6 to −42.27 mV. The niosomal formulation was then loaded to aqueous polymer solution of 30% polyvinyl pyrrolidone (PVP) (MN-1), 30% PVP with 15% poly(vinyl alcohol) (PVA) (2:1) (MN-2), and 30% PVP with 15% PVA (1:1) (MN-3). The cumulative amount of ALS (Q) was in the following order: MN-1 > MN-2 > MN-3. All formulations in this study were stable at room temperature over two months, in terms of moisture content and drug content. In conclusion, a transdermal delivery of ALS niosomes combined in microneedles (MNs) was successfully prepared to provide sustained release of ALS.
Fabrication of Ternary Nanoparticles for Catalytic Ozonation to Treat Parabens: Mechanisms, Efficiency, and Effects on Ceratophyllum demersum L. and Eker Leiomyoma Tumor-3 Cells
Authors: Pattanateeradetch Apiladda , Sakulthaew Chainarong , Angkaew Athaphon , Sutjarit Samak , Poompoung Thapanee , Lin Yao-Tung , Harris Clifford E. , Comfort Steve , Chokejaroenrat Chanat
Published: October 2022

The use of parabens in personal care products can result in their leakage into water bodies, especially in public swimming pools with insufficient water treatment. We found that ferrite-based nanomaterials could catalytically enhance ozone efficiency through the production of reactive oxygen species. Our objective was to develop a catalytic ozonation system using ternary nanocomposites that could minimize the ozone supply while ensuring the treated water was acceptable for disposal into the environment. A ternary CuFe2O4/CuO/Fe2O3 nanocomposite (CF) delivered excellent degradation performance in catalytic ozonation systems for butylparaben (BP). By calcining with melamine, we obtained the CF/g-C3N4 (CFM) nanocomposite, which had excellent magnetic separation properties with slightly lower degradation efficiency than CF, due to possible self-agglomeration that reduced its electron capture ability. The presence of other constituent ions in synthetic wastewater and actual discharge water resulted in varying degradation rates due to the formation of secondary active radicals. 1O2 and •O2− were the main dominant reactive species for BP degradation, which originated from the O3 adsorption that occurs on the CF≡Cu(I)–OH and CF≡Fe(III)–OH surface, and from the reaction with •OH from indirect ozonation. Up to 50% of O3-treated water resulted in >80% ELT3 cell viability, the presence of well-adhered cells, and no effect on the young tip of Ceratophyllum demersum L. Overall, our results demonstrated that both materials could be potential catalysts for ozonation because of their excellent degrading performance and, consequently, their non-toxic by-products.
Development and Perspectives of Thermal Conductive Polymer Composites
Authors: Wang Jiaqi , Hu Lin , Li Wenhao , Ouyang Yuge , Bai Liuyang
Published: October 2022

With the development of electronic appliances and electronic equipment towards miniaturization, lightweight and high-power density, the heat generated and accumulated by devices during high-speed operation seriously reduces the working efficiency and service life of the equipment. The key to solving this problem is to develop high-performance thermal management materials and improve the heat dissipation efficiency of the equipment. This paper mainly summarizes the research progress of polymer composites with high thermal conductivity and electrical insulation, including the thermal conductivity mechanism of composites, the factors affecting the thermal conductivity of composites, and the research status of thermally conductive and electrical insulation polymer composites in recent years. Finally, we look forward to the research focus and urgent problems that should be addressed of high-performance thermal conductive composites, which will provide strategies for further development and application of advanced thermal and electrical insulation composites.
Sulfide-Doped Magnetic Carbon Nanotubes Developed as Adsorbent for Uptake of Tetracycline and Cefixime from Wastewater
Authors: Sereshti Hassan , Beyrak-Abadi Elahe , Esmaeili Bidhendi Mehdi , Ahmad Irfan , Shahabuddin Syed , Rashidi Nodeh Hamid , Sridewi Nanthini , Wan Ibrahim Wan Nazihah
Published: October 2022

In this study, a magnetic solid-phase extraction method was developed based on multi-wall carbon nanotubes decorated by magnetic nanoparticles (Fe3O4) and cadmium sulfide nanoparticles (Fe3O4@MWCNT-CdS) for trace extraction of cefixime and tetracycline antibiotics from urine and drug company wastewater. The adsorbent features were characterized by Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscope (FESEM), and energy dispersive X-ray analysis (EDX). Various effective parameters on the sorption and desorption cycle, such as sorption time, the mass of adsorbent, pH, salt addition, and material ratio, were investigated and optimized. The data were evaluated using isotherm models, and experimental data were well-fitted to both Langmuir (R2 = 0.975) and Freundlich (R2 = 0.985) models. Moreover, kinetic of reaction was agreement with pseudo-second-order (R2 = 0.999) as compared pseudo-first-order (R2 = 0.760). The maximum adsorption capacity for tetracycline and cefixime was achieved at 116.27 and 105.26 mg·g−1, respectively. Hence, the prepared adsorbent can be used as an alternative material for enhanced determination of pharmaceutical substances in biological fluids.
Enhanced Magnetic Hyperthermia Performance of Zinc Ferrite Nanoparticles under a Parallel and a Transverse Bias DC Magnetic Field
Authors: Lucaciu Constantin Mihai , Nitica Stefan , Fizesan Ionel , Filip Lorena , Bilteanu Liviu , Iacovita Cristian
Published: October 2022

The collective organization of magnetic nanoparticles (MNPs) influences significantly their hyperthermic properties, relevant for their in vitro and in vivo applications. We report a systematic investigation of the effects of the concentration and the static bias direct current (DC) magnetic field superposed over the alternating magnetic field (AMF), both in a parallel and perpendicular configuration, on the specific absorption rate (SAR) by using zinc ferrite MNPs. The nonmonotonic dependence of the SAR on the concentration, with a maximum at very small concentrations (c ≤ 0.1 mgFe/mL), followed by a minimum at 0.25 mgFe/mL, and the second maximum of 3.3 kW/gFe at around 1 mgFe/mL, was explained by the passage of the MNPs from a single particle behavior to a collective one and the role of the dipolar interactions. By superposing a static 10 kA/m bias DC field on the AMF we obtained an increase in the SAR for both parallel and perpendicular orientations, up to 4285 W/gFe and 4070 W/gFe, respectively. To the best of our knowledge, this is the first experimental proof of a significant enhancement of the SAR produced by a perpendicular DC field. The effect of the DC field to increase the SAR is accompanied by an increase in the hyperthermia coercive field (HcHyp) for both configurations. No enhancement of the DC fields was noticed for the MNPs immobilized in a solid matrix but the DC field increases the HcHyp only in the parallel configuration. This translates into a higher SAR value for the perpendicular configuration as compared to the parallel configuration. These results have practical applications for magnetic hyperthermia.
Oxygen-Sensitive Photo- and Radioluminescent Polyurethane Nanoparticles Modified with Octahedral Iodide Tungsten Clusters
Authors: Bardin Vyacheslav A. , Vorotnikov Yuri A. , Stass Dmitri V. , Vorotnikova Natalya A. , Shestopalov Michael A.
Published: October 2022

The development of cancer treatment techniques able to cure tumors located deep in the body is an urgent task for scientists and physicians. One of the most promising methods is X-ray-induced photodynamic therapy (X-PDT), since X-rays have unlimited penetration through tissues. In this work, octahedral iodide tungsten clusters, combining the properties of a scintillator and photosensitizer, are considered as a key component of nanosized polyurethane (pU) particles in the production of materials promising for X-PDT. Cluster-containing pU nanoparticles obtained here demonstrate bright photo- and X-ray-induced emission in both solid and water dispersion, great efficiency in the generation of singlet oxygen, and high sensitivity regarding photoluminescence intensity in relation to oxygen concentration. Additionally, incorporation of the cluster complex into the pU matrix greatly increases its stability against hydrolysis in water and under X-rays.
Pro-Inflammatory Cytokines Promote the Transcription of Circular RNAs in Human Pancreatic β Cells
Authors: Kaur Simranjeet , Frørup Caroline , Mirza Aashiq H. , Fløyel Tina , Yarani Reza , Colli Maikel L. , Johannesen Jesper , Størling Joachim , Eizirik Decio L. , Pociot Flemming
Published: October 2022

Circular RNAs (circRNAs) have recently been implicated in impaired β-cell function in diabetes. Using microarray-based profiling of circRNAs in human EndoC-βH1 cells treated with pro-inflammatory cytokines, this study aimed to investigate the expression and possible regulatory roles of circRNAs in human β cells. We identified ~5000 β-cell-expressed circRNAs, of which 84 were differentially expressed (DE) after cytokine exposure. Pathway analysis of the host genes of the DE circRNAs revealed the enrichment of cytokine signaling pathways, indicative of circRNA transcription from inflammatory genes in response to cytokines. Multiple binding sites for β-cell-enriched microRNAs and RNA-binding proteins were observed for the highly upregulated circRNAs, supporting their function as ‘sponges’ or ‘decoys’. We also present evidence for circRNA sequence conservation in multiple species, the presence of cytokine-induced regulatory elements, and putative protein-coding potential for the DE circRNAs. This study highlights the complex regulatory potential of circRNAs, which may play a crucial role during immune-mediated β-cell destruction in type 1 diabetes.
The Extent of Burn Injury Significantly Affects Serum Micro- and Macroelement Concentrations in Patients on the First Day of Hospitalisation
Authors: Gutowska Izabela , Żwierełło Wojciech , Piorun Krzysztof , Skórka-Majewicz Marta , Maciejewska-Markiewicz Dominika , Kupnicka Patrycja , Baranowska-Bosiacka Irena , Dalewski Bartosz , Chlubek Dariusz
Published: October 2022

Burns exceeding 30% of total body surface area (TBSA) result in considerable hypovolemia coupled with the formation and release of inflammatory mediators, leading to subsequent systemic effects known as burn shock. Because of plasma exudation and the associated losses of large quantities of minerals, severe burns can lead to nutritional deficiencies and consequently disrupt homeostasis and metabolism of the entire body. The study group comprised 62 patients, who were divided into 3 groups according to the severity of burns. Serum samples were tested for concentrations of Ca, Mg, Mn, P, K, Zn, Cu, Fe, Se, Na, Cr, Ni, and Al. The mineral concentrations in serum of patients with burn injuries differ significantly from reference values, but this is not affected by the extent of the body burn. There are statistically significant decreases in serum concentrations of elements important for antioxidant protection (Zn, Cu, Se), and significant increases in the concentrations of toxic elements (Al and Ni), which may aggravate the effects associated with the state of burn shock. The Spearman rank correlation analysis did not reveal any statistically significant relationships between the serum concentrations of Mn, Ni, Al, K, Na, P, Mg, Zn, Se, Cr and the affected body surface area and severity of the burn—the values were at the lower end of the reference range. The obtained results indicate that proper nutrition, including elements replenishment, is extremely important in the recovery process of burn patients and time to nutrition is an important factor affecting patient survival after severe burn.
Polypharmacy and Its Association with Dysphagia and Malnutrition among Stroke Patients with Sarcopenia
Authors: Matsumoto Ayaka , Yoshimura Yoshihiro , Nagano Fumihiko , Bise Takahiro , Kido Yoshifumi , Shimazu Sayuri , Shiraishi Ai
Published: October 2022

Evidence on polypharmacy in patients with sarcopenia is lacking. We aimed to examine the association of polypharmacy with swallowing function and nutritional risk in post-stroke patient with sarcopenia. This retrospective cohort study included hospitalized patients diagnosed with sarcopenia who needed convalescent rehabilitation following stroke onset. Study outcomes were the Food Intake Level Scale (FILS) as dysphagia assessment and geriatric nutritional risk index (GNRI) as nutritional status at hospital discharge, respectively. To examine the impact of admission polypharmacy, multivariate linear regression analyses were used to determine whether the number of drugs prescribed at hospital admission was associated with these outcomes. As a result, of the 586 patients enrolled, 257 (mean age 79.3 years, 44.0% male) were diagnosed with sarcopenia and were finally analyzed high admission drug prescription numbers were independently associated with FILS (β = −0.133, p = 0.017) and GNRI (β = −0.145, p = 0.003) at hospital discharge, respectively. Polypharmacy is associated with dysphagia and malnutrition in post-stroke patients with sarcopenia. In addition to the combination of nutritional and exercise therapies, review and optimization of prescription medications may be warranted to treat sarcopenia in order to maximize improvement in outcomes for these patients.
Calf Circumference, a Valuable Tool to Predict Sarcopenia in Older People Hospitalized with Hip Fracture
Authors: Borges Keith , Artacho Reyes , Jodar-Graus Rosa , Molina-Montes Esther , Ruiz-López María Dolores
Published: October 2022

Sarcopenia is an important risk factor for hip fracture in older people. Nevertheless, this condition is overlooked in clinical practice. This study aimed to explore the factors associated with sarcopenia among older patients hospitalized for hip fracture, to identify a predictive model of sarcopenia based on variables related to this condition, and to evaluate the performance of screening tools in order to choose the most suitable to be adopted in routine care of older people with hip fracture. A cross-sectional study was undertaken with 90 patients (mean age 83.4 ± 7.2 years), by assessing sociodemographic and clinical characteristics, anthropometric measures, such as body mass index (BMI) and calf circumference (CC), the functional status (Barthel Index), the nutritional status (MNA-SF), and the adherence to the Mediterranean Diet (MEDAS). Diagnosis of sarcopenia was established according to the criteria of the European Working Group on Sarcopenia in Older People (EWGSOP2). The analysis of variables associated with sarcopenia was performed using multivariate logistic regression models. Clusters of sarcopenia were explored with heatmaps and predictive risk models were estimated. Sarcopenia was confirmed in 30% of hip fracture patients. Variables with the strongest association with sarcopenia were BMI (OR = 0.79 [0.68–0.91], p < 0.05) and CC (OR = 0.64 [0.51–0.81], p < 0.01). CC showed a relatively high predictive capacity of sarcopenia (area under the curve: AUC = 0.82). Furthermore, CC could be a valuable tool to predict sarcopenia risk compared with the currently used screening tools, SARC-F and SARC-CalF (AUC, 0.819 vs. 0.734 and 0.576, respectively). More studies are needed to validate these findings in external study populations.
Proteins from Modern and Ancient Wheat Cultivars: Impact on Immune Cells of Healthy Individuals and Patients with NCGS
Authors: Dieterich Walburga , Schuster Charlotte , Gundel Paulina , Scherf Katharina A. , Pronin Darina , Geisslitz Sabrina , Börner Andreas , Neurath Markus F. , Zopf Yurdagül
Published: October 2022

In non-celiac gluten sensitivity (NCGS), the elimination of wheat results in a clear symptom improvement, but gluten has still not been proven as (the sole) trigger. Due to the increase in the prevalence of gluten-related diseases, the breeding of high-performance wheat cultivars is discussed as a trigger. To analyze the immune stimulation and signal pathways, the immune cells of healthy subjects and patients with NCGS were stimulated with gliadins from wheat, and the expression and secretion of interleukin 1ß (IL1ß) and interleukin 6 (IL6) were studied. To determine the impact of wheat breeding, the monocyte cell line THP1 and human immune cells were stimulated with gliadin, glutenin, and albumin/globulin fractions of ancient and modern cereals, and expression of inflammatory molecules was checked. Immune cells of patients with NCGS showed an increased expression of IL1ß and IL6 after stimulation with gliadins compared to immune cells of healthy controls. Gliadins caused a strong activation of P-STAT3 in immune cells of healthy controls, and inhibitors of JAK and NFκB pathways considerably reduced this response. In addition to gliadins, we further showed that glutenins and albumin/globulins from all wheat cultivars from the last century, and especially from einkorn and spelt, also markedly induced the expression of inflammatory genes in THP1 and human immune cells. There was no correlation between enhanced immune stimulation and ancient or modern cultivars. This does not support the hypothesis that modern wheat breeding is responsible for the increase in gluten-related diseases. An altered immune situation is suggested in patients with NCGS.
Dietary Interventions in Inflammatory Bowel Disease
Authors: Godala Małgorzata , Gaszyńska Ewelina , Zatorski Hubert , Małecka-Wojciesko Ewa
Published: October 2022

Inflammatory bowel disease, which primarily includes ulcerative colitis and Crohn’s disease, is a group of chronic diseases of the gastrointestinal tract. Mainly affecting young people, it is characterized by periods of exacerbation and remission. In recent years, there has been an increase in the prevalence of inflammatory bowel disease worldwide, including Poland. The potential impact of nutrition and selected dietary components that are directly or indirectly involved in the pathogenesis of intestinal lesions in IBD is not fully clear. Evaluating the impact of diet on the course of IBD is very complex due to the fact that regardless of a dietary model adopted, each one is based on consumption of many different food groups which affect one another. However, the growing need to produce dietary recommendations for these patients has prompted the International Organization for the Study of Inflammatory Bowel Disease (IOIBD) to develop nutrition guidelines for the patients. The present paper characterizes the dietary models most commonly discussed in research studies and their potential impact on IBD activity.
Trends in Nutrition Research for Sarcopenia: A Bibliometric Analysis
Authors: Wu Lei , He Kelin , Fang Dandan , Qiu Xiuyue , Xiao Wenhui , Lou Shuhui , Yong Rulin
Published: October 2022

Sarcopenia is age-related, pathophysiological muscle atrophy. Research regarding nutrition treatment of sarcopenia has developed rapidly, particularly as populations age. We evaluated the development of trends in this field using a bibliometric analysis. Articles up to July 2022 were searched in the Scopus database. Bibliographic information from the selected publications, such as countries, citations, world maps, institutions, authors, journals, and keywords, were converted and analyzed automatically using the “bibliometrix” package in R environment (version 4.2.0). We identified 368 Scopus articles from 1998–2021. According to citation analyses, 27 documents received more than 100 citations. Bibliometric analysis based on the literature included in this study revealed that South Korea (61 publications), United States (50), Japan (35), China (30), and Italy (20) contributed the most publications. Tehran University of Medical Science (19 records, 2.55% of articles) contributed the most publications. The most productive author was Landi, with eight articles (2.17% of articles). The publications were located in 196 journals, with Nutrients having the most publications (30, 8.15% of articles). The curves representing keywords “sarcopenia” and “aged” were the most apparent. Our analysis suggested that global nutrition and sarcopenia-related research increased rapidly from 2001 to 2021, demonstrating that this was a promising area of future research that could benefit from continued advances. Future research may focus on the effects of age and sex as well as intervention effectiveness, particularly exercise and nutrition supplementation.
Transfer Phenomena of Nanoliposomes by Live Imaging of Primary Cultures of Cortical Neurons
Authors: Passeri Elodie , Bun Philippe , Elkhoury Kamil , Linder Michel , Malaplate Catherine , Yen Frances T. , Arab-Tehrany Elmira
Published: October 2022

Soft nanoparticles, and in particular, nanoliposomes (NL), have attracted increasing interest for their use in food, nutraceuticals, and in particular, in pharmaceutics for drug delivery. Recent data using salmon lecithin NL suggest that these NL, rich in omega-3 (n-3) fatty acids, can improve the bioavailability and transport of molecules through the blood brain barrier (BBB) to target the brain for the prevention and treatment of neurodegenerative diseases. The objective of this study was to characterize the physicochemical properties and analyze the transfer phenomena of salmon lecithin NL over time in neurons to better understand the behavior of NL in an intracellular environment. To test this, primary cultures of cortical neurons from rat embryos were incubated with salmon lecithin NL from day 3 after cell culture, for up to 104 h. The physicochemical properties of NL such as size, speed, morphology and the diffusion coefficient in the live cultures, were studied over time. Image analysis of cell morphology showed dendritic growth and neuronal arborization after 48 h of exposure to NL, for up to 104 h. Results showed an NL stability in size, speed and diffusion coefficient over time, with a peak at 48 h, and then a return to baseline value at the end of incubation. The average speed and diffusion coefficient achieved provided important information on the mode of entry of NL into neurons, and on the slow diffusion rate of NL into the cells. Analysis of videos from 2 h to 104 h showed that significant levels of NL were already internalized by neurons after 3 h incubation. NL appearance and intracellular distribution indicated that they were packed in intracellular compartments similar to endocytic vesicles, suggesting internalization by an active endocytic-like process. The results obtained here demonstrate internalization of NL by cortical neurons by an active endocytic-like process, and suggest the potential use of NL for time-release of therapeutics aimed towards prevention or treatment of neurodegenerative diseases.
The Uncertainty Principle and the Minimal Space–Time Length Element
Authors: Escors David , Kochan Grazyna
Published: October 2022

Quantum gravity theories rely on a minimal measurable length for their formulations, which clashes with the classical formulation of the uncertainty principle and with Lorentz invariance from general relativity. These incompatibilities led to the development of the generalized uncertainty principle (GUP) from string theories and its various modifications. GUP and covariant formulations of the uncertainty principle are discussed, together with implications for space–time quantization.
Cytogenetics and Consequences of Polyploidization on Different Biotic-Abiotic Stress Tolerance and the Potential Mechanisms Involved
Authors: Islam Md Mazharul , Deepo Deen Mohammad , Nasif Saifullah Omar , Siddique Abu Bakar , Hassan Oliul , Siddique Abu Bakar , Paul Narayan Chandra
Published: October 2022

The application of polyploidy in sustainable agriculture has already brought much appreciation among researchers. Polyploidy may occur naturally or can be induced in the laboratory using chemical or gaseous agents and results in complete chromosome nondisjunction. This comprehensive review described the potential of polyploidization on plants, especially its role in crop improvement for enhanced production and host-plant resistance development against pests and diseases. An in-depth investigation on techniques used in the induction of polyploidy, cytogenetic evaluation methods of different ploidy levels, application, and current research trends is also presented. Ongoing research has mainly aimed to bring the recurrence in polyploidy, which is usually detected by flow cytometry, chromosome counting, and cytogenetic techniques such as fluorescent in situ hybridization (FISH) and genomic in situ hybridization (GISH). Polyploidy can bring about positive consequences in the growth and yield attributes of crops, making them more tolerant to abiotic and biotic stresses. However, the unexpected change in chromosome set and lack of knowledge on the mechanism of stress alleviation is hindering the application of polyploidy on a large scale. Moreover, a lack of cost–benefit analysis and knowledge gaps on the socio-economic implication are predominant. Further research on polyploidy coupling with modern genomic technologies will help to bring real-world market prospects in the era of changing climate. This review on polyploidy provides a solid foundation to do next-generation research on crop improvement.
Essential Oil Composition and Stable Isotope Profile of Osmorhiza occidentalis Torr. (Apiaceae) from Utah
Authors: Wilson Tyler M. , Murphy Brett J. , Ziebarth Emma A. , Poulson Ariel , Packer Chris , Carlson Richard E.
Published: October 2022

Osmorhiza occidentalis Torr. is an essential-oil-bearing plant in the Apiaceae family. Volatile oil was produced through steam distillation (n = 3) of the above ground plant parts and was analyzed by gas chromatography (GC/FID, GC/MS), and gas chromatography/isotope ratio mass spectrometry (GC/IRMS) to establish the essential oil composition and stable isotope profile. The resulting essential oils were found to be comprised of 33 volatile compounds. Prominent volatile compounds include methyl chavicol (avg. 61.6%), (Z)-β-ocimene (avg. 14.7%), sabinene (avg. 10.5%), and γ-terpinene (avg. 2.8%). Stable isotope values were determined for prominent volatile compounds, including methyl chavicol, (Z)-β-ocimene, sabinene, and γ-terpinene. Values for δ2H range from −393.479 (avg. sabinene) to −171.516 (avg. methyl chavicol). Those for δ13C range from −35.957 (avg. methyl chavicol) to −30.820 (avg. (Z)-β-ocimene). The essential oil yield was 0.12% (w/w). The current study establishes for the first time, to the best knowledge of the authors, the essential oil yield, essential oil composition, and stable isotope profile of prominent volatile compounds extracted from the above-ground portions of O. occidentalis. These results provide insight into the volatile chemical composition produced by the plant and provide fundamental data for substantiation of ethnobotanical applications.
Biotechnological Potential of Different Organs of Mistletoe (Viscum album L.) Collected from Various Host Tree Species in an Urban Area
Authors: Skrypnik Liubov , Feduraev Pavel , Golovin Anton , Maslennikov Pavel , Belov Nikolay , Matveev Matvei , Pungin Artem
Published: October 2022

From an economic and ecological standpoint, it is crucial to investigate the biologically active compounds of mistletoe plants, which are currently discarded by pruning urban mistletoe-infested trees. In the present study, the content of phenolic compounds, triterpenic and organic acids, as well as the antioxidant activity of the extracts of various mistletoe organs (leaves, stems, and fruits) collected from the most infested tree species were investigated. The mistletoe samples collected from Betula pendula, Acer platanoides, Crataegus monogyna, and Sorbus aucuparia showed the highest content of phenolic acids and flavonoids as well as antioxidant activity, as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing/antioxidant power (FRAP) assays. The leaves and stems of mistletoe from Tilia cordata were characterized by a high content of triterpenic acids (oleanolic, ursolic, and betulinic). The leaves and fruits of mistletoe plants from Populus nigra and Salix alba contained a high concentration of organic acids, particularly succinic and citric acids. Compared to stem and leaf extracts, the antioxidant activity of the mistletoe fruit extracts was 1.5–3 times higher. The obtained results indicate that mistletoe is a valuable raw material and can be used as a source of phenolic compounds and triterpenic and organic acids, as well as for producing extracts with antioxidant properties.
UAV Image-Based Crop Growth Analysis of 3D-Reconstructed Crop Canopies
Authors: Nielsen Karsten M. E. , Duddu Hema S. N. , Bett Kirstin E. , Shirtliffe Steve J.
Published: October 2022

Plant growth rate is an essential phenotypic parameter for quantifying potential crop productivity. Under field conditions, manual measurement of plant growth rate is less accurate in most cases. Image-based high-throughput platforms offer great potential for rapid, non-destructive, and objective estimation of plant growth parameters. The aim of this study was to assess the potential for quantifying plant growth rate using UAV-based (unoccupied aerial vehicle) imagery collected multiple times throughout the growing season. In this study, six diverse lines of lentils were grown in three replicates of 1 m2 microplots with six biomass collection time-points throughout the growing season over five site-years. Aerial imagery was collected simultaneously with each manual measurement of the above-ground biomass time-point and was used to produce two-dimensional orthomosaics and three-dimensional point clouds. Non-linear logistic models were fit to multiple data collection points throughout the growing season. Overall, remotely detected vegetation area and crop volume were found to produce trends comparable to the accumulation of dry weight biomass throughout the growing season. The growth rate and G50 (days to 50% of maximum growth) parameters of the model effectively quantified lentil growth rate indicating significant potential for image-based tools to be used in plant breeding programs. Comparing image-based groundcover and vegetation volume estimates with manually measured above-ground biomass suggested strong correlations. Vegetation area measured from a UAV has utility in quantifying lentil biomass and is indicative of leaf area early in the growing season. For mid- to late-season biomass estimation, plot volume was determined to be a better estimator. Apart from traditional traits, the estimation and analysis of plant parameters not typically collected in traditional breeding programs are possible with image-based methods, and this can create new opportunities to improve breeding efficiency mainly by offering new phenotypes and affecting selection intensity.
Morphological and Physiological Responses of Hybrid Aspen (Populus tremuloides Michx. × Populus tremula L.) Clones to Light In Vitro
Authors: Kondratovičs Toms , Zeps Mārtiņš , Rupeika Diāna , Zeltiņš Pauls , Gailis Arnis , Matisons Roberts
Published: October 2022

Micropropagation of fast-growing tree genotypes such as the hybrid aspen (Populus tremuloides Michx. × Populus tremula L.) is increasing. The efficiency of micropropagation depends on the luminaires, hence luminescent electric diodes (LED), which emit light of a narrow spectrum, are gaining popularity. Mostly, different LEDs are combined to increase the photosynthetic efficiency. However, light also acts as an environmental signal, which triggers specific responses in plants, which are genotype specific, and regarding hybrid aspen, are likely affected by heterosis. In this study, morphological and physiological responses of clones of hybrid aspen with contrasting field performance to the spectral composition of illumination were studied in vitro. Among the 15 variables measured, area of leaves and concentration and ratio of chlorophyll a and b explained most of the variance (58.6%), thereby linking a specific combination of traits to productivity. These traits and their responses to light were affected by heterosis, as indicated by the clone-treatment interaction, particularly for the clone’s moderate productivity. The top-performing clones were little sensitive to illumination due to efficient photosystems. Nevertheless, illumination with wider spectral composition had generally positive effects on plantlet performance. Accordingly, clone-specific illumination protocols and luminaries capable of it are advantageous for the efficiency of micropropagation of hybrid aspen.
Biological and Physicochemical Characterization of Self-Adhesive Protective Coating Dental Restorative Material after Incorporation of Antibacterial Nanoparticles
Authors: Gul Nazish , Idrees Qura Tul Ain , Fareed Muhammad Amber , Mian Salman Aziz , Nasim Hafiz Muhammad Owais , Naz Fariha , Aldahlan Bashayer , Khan Abdul Samad
Published: October 2022

This study evaluated the physicochemical and antibacterial properties of EQUIATM coat liquid (E) after incorporation of zinc oxide (ZnO) and titanium dioxide (TiO2) nanoparticles. ZnO and TiO2 (1 wt.% and 2 wt.%) were dispersed in EQUIA coat. Principal component analysis (PCA) and cluster analysis were performed to visualize systemic variation. Antibacterial activity was evaluated by colony-forming units and crystal violet staining using Streptococcusmutans and Lactobacillusacidophilus after 24 h, 48 h, and 72 h, and the microstructure was studied by scanning electron microscopy. The weight change was analyzed at 1 and 21 days. The PCA for TiO2- and ZnO-based groups showed 100% variance at all spectral ranges at 600–800/cm and 800–1200/cm, whereas 1200–1800/cm and 2700–3800/cm spectral regions demonstrated 99% variance. The absorbance values were significant (p < 0.05) for both nanoparticles-based adhesives, and the specimens with 2 wt.% ZnO showed the maximum response by minimum bacterial attachment, and the control group showed the least response by maximum attachment. The weight change percentage was reduced after the incorporation of antibacterial nanoparticles. It is suggested that EQUIATM coat containing nanoparticles exhibits promising results, and it may be recommended to clinically use as an improved coating material.
Advances in the Measurement of Polymeric Colorimetric Sensors Using Portable Instrumentation: Testing the Light Influence
Authors: Martínez-Aviño Adria , de Diego-Llorente-Luque Maria , Molins-Legua Carmen , Campíns-Falcó Pilar
Published: October 2022

Sustainable and green sensors based on polydimethyl siloxane (PDMS) or cellulose polymers, as a case of study of the use of portable instrumentation joined to a smartphone, have been tested. A smartphone camera was used to obtain images and was also coupled to a minispectrometer, without and with an optical fiber probe to register spectra. To study light influence on the analytical signal, light-emitting diode (LED), halogen light and daylight have been assayed. A corrective palette of 24 colors and a set with 45 colors from different color ranges were used as the validation set. The results indicated that halogen light was the best option to obtain the spectra. However, for digital image analysis, it was the LED light that gave a greater approximation of the RGB values of the real colors. Based on these results, the spectra and the RGB components of PDMS solid sensors doped with 1,2-naphtoquinone-4-sulfonate (NQS) for the determination of ammonium in water or urea in urine, PDMS doped with Griess reagent for developing the assay of nitrite in waters and cellulose sensors for the determination of hydrogen sulfide in the atmospheres have been obtained. The results achieved were good in terms of sensitivity and linearity and were comparable to those obtained using a laboratory benchtop instrument. Several rules for selecting the most suitable light source to obtain the spectra and/or images have been established and an image correction method has been introduced.
Adhesive Strength in Dentin Conditioned with 18% Ethylenediaminetetraacetic Acid versus 35% Phosphoric Acid: In Vitro Study with 1-Year Artificial Aging
Authors: Alcántara-Obispo Esther , Santander-Rengifo Flor , Ladera-Castañeda Marysela , López-Gurreonero Carlos , Castro Pérez-Vargas Antonieta , Cornejo-Pinto Alberto , Cervantes-Ganoza Luis , Cayo-Rojas César
Published: October 2022

The success and longevity of a resin composite restoration is determined by its good bonding to the tooth structure, with the adhesion being a challenge to dentin due to its complexity and structural heterogeneity. The present study aimed to compare the adhesive strength of dentin conditioned with 18% ethylenediaminetetraacetic acid (EDTA) versus 35% phosphoric acid (H3PO4) in human premolars. Materials and Methods: This in vitro experimental study was performed on 40 human premolars. The occlusal thirds were sectioned and randomly placed into four groups according to the type of dentin conditioning: Group 1 (control), Group 2 (18% EDTA), Group 3 (35% H3PO4) and Group 4 (18% EDTA plus 35% H3PO4). Then, 10,000 thermocycles between 5 +/− 2 °C and 55 +/− 2 °C were applied. Adhesive strength was tested by shearing with a digital universal testing machine at a crosshead speed of 0.75 mm/min. The values obtained were analyzed in megapascals (MPa). The mean and standard deviation were used as measures of central tendency and dispersion. In addition, a one-factor intergroup ANOVA test was applied with Tukey’s post hoc test considering a significance level of p < 0.05. Results: The 18% EDTA and 18% EDTA plus 35% H3PO4 showed significantly higher adhesive strength compared to the control group that did not receive dentin conditioning (p = 0.047 and p < 0.001, respectively). However, the group conditioned with 18% EDTA did not present significant differences compared to the group conditioned with 35% H3PO4 (p = 0.997). In addition, the group conditioned with 18% EDTA plus 35% H3PO4 showed significantly higher adhesive strength compared to the groups conditioned with 18% EDTA (p = 0.002) and 35% H3PO4 (p = 0.001). Conclusion: The adhesion of bulk fill resin composite to dentin was favorable when preconditioning was performed using 18% EDTA followed by 35% H3PO4. In contrast, when both etchants were used separately, the bulk fill resin composite showed similar bond strength values in both cases, but significantly lower compared to their sequential application.
Enhanced Optical Confinement Enriching the Power Conversion Efficiency of Integrated 3D Grating Organic Solar Cell
Authors: Zohar Moshe , Avrahamy Roy , Hava Shlomo , Milgrom Benny , Rimon Evyatar
Published: October 2022

In this paper, we examine the impact of three-dimensional grating layers embedded at selected locations in an organic solar cell structure to obtain enhanced efficiency. The design, simulations, and optimizations were carried out using an in-house tool based on the rigorous coupled-wave analysis (RCWA) method developed on the MATLAB R2019a platform. An optimal organic solar cell structure design with a top grating layer exhibited an increase of 7.47% in the short-circuit current density compared to an organic solar cell structure with a smooth top layer. The power conversion efficiency (PCE) increase was mainly due to increased light confinement in the thin absorbing layer. Adding an embedded grating layer in the absorption layer resulted in a significant increase in the absorptance spectral bandwidth, where the short-circuit current density increased by 10.88%. In addition, the grating cells yielded a substantial improvement in the cell’s conical absorptance since the existence of a surface plasmon polariton (SPP) in the back metal gratings increases the confinement properties. Further, the effect of a pyramid-shaped embedded grating array was a slight improvement in the PCE compared to the rectangular-shaped grating arrays. We showed that a pyramid-grating can act as a nano black-body layer, increasing the absorption for a wide range of azimuthal and polar incident angles.
The Total Low Frequency Oscillation Damping Method Based on Interline Power Flow Controller through Robust Control
Authors: Zhao Jingbo , Xu Ke , Li Zheng , Wu Shengjun , Wang Dajiang
Published: October 2022

The interline power flow controller (IPFC) can control the active power and reactive power of different lines in power system. To utilize the flexible control ability of IPFC and increase the damping characteristic of its controller AC system, this paper proposes a low-frequency oscillation (LFO) suppress method through IPFC. The LFO suppress method is designed by adding supplementary signals to the outer current control loop of IPFC. In addition to adding supplementary active power signals, the reactive supplementary signals are also added to related control loop, which is the total control scheme. To obtain the power system’s small signal model, the identification technology based on the PRONY algorithm is used. In addition, the robust control theory is also applied to make the controllers more adaptive. To verify the effectiveness of the proposed method, two controllers including both the active and reactive controllers are designed for in PSCAD software. Furthermore, the simulation results prove the proposed method can reach a better control effect and is also of robustness.
BRIDE v2: A Validated Collection of Genes Involved in the Mammalian Brain Response to Low-Dose Ionizing Radiation
Authors: Karapiperis Christos , Vasileiou Dimitrios , Angelis Lefteris , Ouzounis Christos A.
Published: October 2022

There is significant interest in the response of the mammalian brain to low-dose ionizing radiation (LDIR), mainly examined by gene or protein expression, with applications in radiation safety on Earth, the atmosphere and outer space. Potential associations of molecular-level responses with sensory or cognitive defects and neurodegenerative diseases are currently under investigation. Previously, we have described a light-weight approach for the storage, analysis and distribution of relevant datasets, with the platform BRIDE. We have re-implemented the platform as BRIDE v2 on the cloud, using the bioinformatics infrastructure ELIXIR. We connected the annotated list of 3174 unique gene records with modern omics resources for downstream computational analysis. BRIDE v2 is a cloud-based platform with capabilities that enable researchers to extract, analyze, visualize as well as export the gene collection. The resource is freely available online at .
Measuring Kinetics in Flow Using Isoperibolic Flow Calorimetry
Authors: Frede Timothy Aljoscha , Greive Moritz , Kockmann Norbert
Published: October 2022

Continuous flow calorimeters are a promising tool in process development and safety engineering, particularly for flow chemistry applications. An isoperibolic flow calorimeter is presented for the characterization of exothermic reactions. The calorimeter is adapted to commercially available plate microreactors made of glass and uses Seebeck elements to quantify the heat of reaction. For automation of calibration procedures and calorimetric measurements, the device is connected to a lab automation system. Reaction enthalpy of exothermic reactions is determined via an energy balance of the entire calorimeter. Characterization of reaction kinetics is carried out via a local balancing of the individual Seebeck elements without changing the experimental setup, while using the previous measurements and additional ones at higher flow rates. The calorimeter and the associated measurement procedures were tested with the oxidation of sodium thiosulfate using hydrogen peroxide. Reaction enthalpy was determined to be 594.3 ± 0.7 kJ mol−1, which is within the range of literature values.
Is Scripture a Gift? Reflections on the Divine-Ecclesial Provision of the Canon
Authors: East Brad
Published: October 2022

This article investigates whether the canon of Christian Holy Scripture is properly understood as a gift and, if so, what theological implications this might entail. Following the introduction, the article has three main sections. The first section proposes an expanded grammar by which to describe the production and reception of the canon in and by the church, under the superintending sovereignty of the divine will and action. The second section offers a guide to recent inquiry into “the gift” in the fields of philosophy and theology, particularly those theories that might prove useful for applying the concept of “gift” to Scripture. The third section unfolds a normative account of the Christian canon as a gift of the triune God to his people and through his people, thus making sense of the long-standing liturgical practice of responding to the reading of the sacred page in the public assembly with a cry of thanks to God.