bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Anomalies and Neurodevelopment in Children Exposed to ZIKV during Pregnancy
Authors: Guardado Kathia , Varela-Cardoso Miguel , Pérez-Roa Verónica Ofelia , Morales-Romero Jaime , Zenteno-Cuevas Roberto , Ramos-Ligonio Ángel , Guzmán-Martínez Oscar , Sampieri Clara L. , Ortiz-Chacha Christian S. , Pérez-Varela Rosybet , Mora-Turrubiate Cristina Fernanda , Montero Hilda
Published: August 2022

Zika virus (ZIKV) infection in pregnancy is associated with birth and developmental alterations in infants. In this study, clinical records of 47 infants whose mothers had Zika during pregnancy or clinical manifestations compatible with Zika were reviewed. A description of the infants’ anomalies was established, and a neurodevelopmental assessment was performed on 18 infants, using the Evaluation of Infant Development (EDI for its initialism in Spanish) and DDST-II (Denver Developmental Screening Test II) tests. From his sample, 74.5% of the infants evaluated had major anomalies and 51.9% had minor anomalies. The incidence of major anomalies, related to trimester of pregnancy, was 84.2% for the first trimester, 77.8% for the second trimester, and 37.5% in the third trimester. A similar trend was observed in the frequency of infants without anomalies and was less evident in the incidence of minor anomalies (p = 0.016). Through neurodevelopmental assessments, EDI identified 27.8% of infants as having normal development, while 55.5% of affected infants had developmental delay, and 16.7% were at risk for developmental delay. The DDSST-II showed that 77.7% infants had delay in the gross motor and language area, 88.8% in the fine-adaptative motor area, and 72.2% in the personal–social area. In this work, children of mothers with ZIKV infection during pregnancy may have major or minor anomalies regardless of the trimester of pregnancy in which the infection occurred. The neurodevelopmental assessment shows that ZIKV can cause a developmental delay in infants with the fine-adaptative motor area being the most affected.
Subjective Experiences of At-Risk Children Living in a Foster-Care Village Who Participated in an Open Studio
Authors: Bat Or Michal , Zusman-Bloch Reut
Published: August 2022

The open studio art therapy model offers a space for free creation; in this space, the art therapist supports the participants’ art process. According to this model, the creative process is the central component of the therapeutic work. This qualitative study seeks to learn, through an analysis of interviews and artwork, about the subjective experiences of at-risk children living in a foster-care village who participated in an open studio. In addition, it seeks to identify changes in the artwork over time. This study involves a qualitative thematic analysis, while the analysis of visual data is based on the phenomenological approach to art therapy. The data include interviews and 82 artworks of five participants, aged 7–10 years. Five main themes emerged from the analysis of the visual and verbal data: (a) engaging in relationships; (b) moving along the continuum from basic, primary, art expressions (e.g., smearing, scribbling, etc.) to controlled expressions; (c) visibility, on a range between disclosure and concealment; (d) holding versus falling/instability; and (e) experiencing and expressions of change. The discussion expands on the themes in relation to key concepts in the field of psychodynamic psychotherapy and art therapy. It also examines the unique characteristics of this population in reference to empirical studies on developmental trauma and challenges of out-of-home placement. Finally, it discusses the study’s limitations and presents recommendations for further research.
It’s Not Just about Bicycle Riding: Sensory-Motor, Social and Emotional Benefits for Children with and without Developmental Disabilities
Authors: Schoen Sarah A. , Ferrari Vincentia , Valdez Andrea
Published: August 2022

Background: A developing area for therapy is teaching children to ride a bicycle. Little has been written about the effectiveness of these programs. This study explored outcomes from participation in a novel bicycle riding program for children with a wide array of developmental challenges. Method: Two studies were conducted; a nonconcurrent, multiple baseline design with four participants and a pretest–posttest single group with 15 children. Results: Study 1 participants improved on broad jump. Balance positions showed variable responses. Study 2 participants showed significant improvement on broad jump, and a trend toward significance walking forwards and backwards. Parents reported improvement in following rules, participating in daily routines, interacting with peers, and feeling good about him/herself and a change in child’s participation in community and extracurricular activities. All children improved in glide time or achieved independent riding. Conclusions: Preliminary evidence was found for the effectiveness of one approach for developing motor and social skills within the context of learning to ride a bicycle. Children over the age of 6 years were able to ride a two-wheeled bicycle at the end of the program. Participation suggested improvement in motor coordination and dynamic balance as well as changes in measures of social interaction and self-esteem.
Craniofacial Growth at Age 6–11 Years after One-Stage Cleft Lip and Palate Repair: A Retrospective Comparative Study with Historical Controls
Authors: Benitez Benito K. , Weibel Seraina K. , Halbeisen Florian S. , Lill Yoriko , Nalabothu Prasad , Tache Ana , Mueller Andreas A.
Published: August 2022

Background: Primary alveolar bone grafting inhibits craniofacial growth. However, its effect on craniofacial growth in one-stage cleft lip and palate protocols is unknown. This study investigated whether primary alveolar bone grafting performed during one-stage unilateral cleft lip and palate repair negatively affects growth up to 6–11 years old. Methods: The craniofacial growth, dental arch relationship and palatal morphology at 6–11 years old in children with unilateral cleft lip and palate were compared retrospectively. Two cohorts after a one-stage protocol without (Group A) and with (Group B) primary bone grafting at the same center were compared. Further, cephalometric measurements for growth were compared with an external cohort of a one-stage protocol and a heathy control. Results: Group A comprised 16 patients assessed at 6.8 years (SD 0.83), and Group B comprised 15 patients assessed at 9 years (SD 2.0). Cephalometric measurements indicated similar sagittal maxillary growth deficits and a significant deviation in maxillary inclination in both groups compared to the healthy group. Moderate to severe changes in palatal morphology were observed in 70% of the members in both groups. Conclusion: Omitting primary alveolar bone grafting under the one-stage protocol with two-flap palatoplasty studied did not improve growth at 6–11 years. The results implicate two-flap palatoplasty with secondary healing as having greater adverse effects on growth than primary alveolar bone grafting. Dental and palatal morphology was considerably compromised regardless of primary alveolar bone grafting.
Pine-Fiber-Derived Carbon@MnO@rGO as Advanced Anodes for Improving Lithium Storage Properties
Authors: Huang Qinyuan , Feng Bing , Deng Wentao , Zhang Xiang , Liu Gonggang , Su Geng , Chang Shanshan , Hu Jinbo , Liu Yuan , Xiao Gang
Published: August 2022

Fiber carbon (FC) is a potential fast-chargeable anode, which is attributable to the many nanopores in the cell wall. Herein, a strategy for an FC@MnO@rGO composite is proposed to combine pine-based FCs with suitable MnO nanoparticles, which are enfolded by the reduced graphene oxide (rGO). The magic conception is performed to join the advantages of conductive FC skeleton, high theoretical capacity of MnO and high flexibility and conductivity of rGO. Compared to FC, FC@MnO@rGO composite materials have presented superior lithium storage properties, exhibiting an initial discharge capacity of 1191.8 mAh g−1 and charge capacity of 643.5 mAh g−1. It is worth attention that the FC@MnO@rGO shows a reversible capacity of 304.2 mAh g−1 at a current density of 2 A g−1. Furthermore, it delivers an improved capacity retention of 67% at 400 mA g−1 over 400 cycles.
Influence of the Retrograde Filling Material on the Success of Periapical Surgery. Systematic Review and Meta-Analysis by Groups
Authors: Amador-Cabezalí Alicia , Pardal-Peláez Beatriz , Quispe-López Norberto , Lobato-Carreño María , Sanz-Sánchez Ángela , Montero Javier
Published: August 2022

Periapical surgery is a procedure indicated for the treatment of persistent apical periodontitis in an endodontically treated tooth and when retreatment has not been successful or not feasible. Various materials have been suggested and tested for this purpose: amalgam, MTA, and zinc-eugenol oxide cements, among others. The objective of the present study was to evaluate the results regarding the success of the periapical surgery and if the retrograde filling material used influences this success rate. A systematic review of the literature on the prognosis of periapical surgery was performed. The following PICO question was asked: “In patients who require periapical surgery (patient), is the retrograde filling (comparison) material used in apicoectomy (intervention) determining for the success of the treatment (outcome)?” Periapical surgery achieves successful results in 84% of cases. All the materials compared obtain satisfactory results. The results of the meta-analysis show that there are no statistically significant differences between MTA compared to SuperEBA (1.37 (95% CI: 0.87, 2.15)) or RRM (0.99 (95% CI: 0.51, 1.94)) but with MRI (1.93 (95% CI: 1.22, 3.05)), where the results favour MTA. In general, the most modern materials obtain better results than amalgam. Surgical techniques with magnification obtain better results (12.3% failure) than conventional techniques (22.93% failure). Periapical surgery is a procedure with a high success rate. The use of all the materials compared obtains satisfactory results. Magnification surgery techniques perform better than conventional techniques.
Evaluating Strip and No-Till Maintenance of Perennial Groundcovers for Annual Grain Production
Authors: Bartel Cynthia A. , Moore Kenneth J. , Fei Shui-zhang , Lenssen Andrew W. , Hintz Roger L. , Kling Samantha M.
Published: August 2022

Perennial groundcover (PGC) merges scalable soil and water conservation with high-yielding row crops, enhancing ecosystem services of annual grain crop production. However, ineffective groundcover suppression increases competition between the groundcover and row crop, reducing row crop grain yield. The objective of this study was to assess the effectiveness of three Kentucky bluegrass (KBG) groundcover suppression methods each at narrow and wide widths on maize (Zea mays L.) growth and development in evenly spaced PGC, compared to alternating PGC swards and a no-PGC conventional tillage control. Suppression methods for evenly spaced PGC included two different strip tillage implements, completing either shallow or deep soil fracture, for mechanical suppression and a banded sprayer for no-tillage chemical suppression. We measured weekly for maize plant height, phenological stage of development, reflected red:far-red (R:FR) ratio, early vegetative and final plant density, grain and stover yield, yield components of kernel rows ear−1, kernels row−1, kernels ear−1, ear length, kernel weight, grass frequency, and weed community. In 2020, maize grain yield in the alternating PGC swards (11.38 Mg ha−1) was similar to the control (12.78 Mg ha−1) and greater than in the evenly spaced groundcover (9.62 Mg ha−1). Maize grain yield was similar for systems in 2021 (7.41 Mg ha−1), due to drought and high coefficient of variation. Weed community was similar for systems in both years. A maize competition response was observed for both suppression widths across methods. Groundcover dormancy may be needed in conjunction with effective chemical and/or mechanical groundcover suppression to support maize production in PGC.
Synthesis and Characterization of Silver Nanoparticle-Polydimethylsiloxane (Ag-NP-PDMS) Stretchable Conductive Nanocomposites
Authors: Jamali Abdul Rauf , Bhatti Jahanzeb , Khan Waseem , Akther Faheem , Batool Madiha , Batool Razia , Daoush Walid M.
Published: August 2022

A number of different research methodologies have been developed to increase the conductivity and mechanical properties of stretchable or flexible conductors. One of the promising techniques recommended for applying metallic nanoparticles (NPs) to PDMS (polydimethylsiloxane) substrate is to develop a thin-film that gives possible conductivity and good mechanical strain. This article discusses the preparation of silver nanoparticles using the chemical reduction method with silver nitrate as the precursor, and uses glucose as a reducing agent. In addition, polyvinyl pyrrolidone (PVP) is used to prevent the nanoparticles’ oxidation and agglomeration once they have been synthesized successfully. Moreover, we utilize the power of diethylamine to accelerate the evolution of nanoparticles, and deionized water is used to prevent any possible contamination. The prepared Ag-NPs are then deposited on the solidified PDMS substrate through sintering. A multimeter is used to measure the electrical resistance. Ag-NPs are confirmed by UV-Vis at a 400-nm peak. Furthermore, we discuss the surface morphologies, particle sizes and thicknesses of the film and substrate when studied using different microscopy techniques. The prepared stretchable conductor is found to be suitable to use in biosensing and electronic devices.
Metastable Crystallization by Drop Impact
Authors: Nishigaki Akari , Maruyama Mihoko , Tanaka Shun-ichi , Yoshikawa Hiroshi Y. , Imanishi Masayuki , Yoshimura Masashi , Mori Yusuke , Takano Kazufumi
Published: August 2022

It has been reported that cavitation bubbles (air–liquid interface) by femtosecond laser and ultrasonic irradiations are effective for metastable phase crystallization in polymorph control. It has also been noted that cavitation bubbles are generated by mechanical shock when dropping a vial. Here we describe the crystallization of acetaminophen by drop impact. In the condition where spontaneous nucleation did not occur, the drop impact produced the metastable form (form II) and trihydrate. This supports the potency of the air–liquid interface in metastable phase formation. Furthermore, crystallization by drop impact is a completely new phenomenon, and new developments are expected in the future.
The Creation of the Suppressive Cancer Microenvironment in Patients with HPV-Positive Cervical Cancer
Authors: Chaberek Katarzyna , Mrowiec Martyna , Kaczmarek Magdalena , Dutsch-Wicherek Magdalena
Published: August 2022

The development of malignancy is closely connected with the process of cancer microenvironment remodeling. As a malignancy develops, it stimulates the creation of the suppressive microenvironment of the tumor through the presence of cells that express membrane proteins. These proteins are secreted into the cancer microenvironment, where they enable tumor growth. In patients with cancer of the cervix, the development of the disease is also linked to high-risk HPV (hr-HPV) infection. Such infections are common, and most clear spontaneously; however, a small percentage of these infections can persist and progress into precancerous cervical intraepithelial neoplasia and invasive cervical carcinoma. Consequently, it is assumed that the presence of hr-HPV infection alone is not sufficient for the development of cancer. However, chronic HPV infection is associated with the induction of the remodeling of the microenvironment of the epithelium. Furthermore, the local microenvironment is recognized as a cofactor that participates in the persistence of the HPV infection and disease progression. This review presents the selected immune evasion mechanisms responsible for the persistence of HPV infection, beginning with the delay in the virus replication process prior to the maturation of keratinocytes, the shift to the suppressive microenvironment by a change in keratinocyte immunomodulating properties, the alteration of the Th1/Th2 polarization of the immune response in the microenvironment, and, finally, the role of HLA-G antigen expression.
Lowering of the Neonatal Lung Ultrasonography Score after nCPAP Positioning in Neonates over 32 Weeks of Gestational Age with Neonatal Respiratory Distress
Authors: Perri Alessandro , Fattore Simona , D’Andrea Vito , Sbordone Annamaria , Patti Maria Letizia , Nobile Stefano , Tirone Chiara , Giordano Lucia , Tana Milena , Priolo Francesca , Serrao Francesca , Riccardi Riccardo , Prontera Giorgia , Vento Giovanni
Published: August 2022

Respiratory distress (RD) is one of the most common causes of admission to the neonatal intensive care unit. Correct diagnosis and timely intervention are crucial. Lung ultrasonography (LU) is a useful diagnostic tool for the neonatologist in the diagnosis of RD; the neonatal lung ultrasonography score (nLUS) can be used in the diagnostic process, but some authors hypothesise that it is also useful for the management of some neonatal RD. The aim of this study is to analyse the changes in nLUS score before (T0) and after (T1) the start of respiratory support with nasal CPAP in neonates over 32 weeks of age with RD. Thirty-three newborns were enrolled in this retrospective study. LU was performed before and after the start of CPAP. The median nLUS scores at T0 and T1 were 9 (IQR 7–12) and 7 (IQR 4–10), respectively, and showed a significant difference (p < 0.001). The magnitude of reduction in nLUS score, expressed as a percentage, was inversely related to the need for subsequent administration of exogenous surfactant. The study suggests the usefulness of the nLUS score in assessing the response to CPAP in neonates over 32 weeks gestational age.
Nutritional Evaluation of Sea Buckthorn “Hippophae rhamnoides” Berries and the Pharmaceutical Potential of the Fermented Juice
Authors: El-Sohaimy Sobhy A. , Shehata Mohamed G. , Mathur Ashwani , Darwish Amira G. , Abd El-Aziz Nourhan M. , Gauba Pammi , Upadhyay Pooja
Published: August 2022

Sea buckthorn is a temperate bush plant native to Asian and European countries, explored across the world in traditional medicine to treat various diseases due to the presence of an exceptionally high content of phenolics, flavonoids and antioxidants. In addition to the evaluation of nutrients and active compounds, the focus of the present work was to assess the optimal levels for L. plantarum RM1 growth by applying response surface methodology (RSM), and to determine the impact of juice fermentation on antioxidant, anti-hypertension and anticancer activity, as well as on organoleptic properties. Sea buckthorn berries were shown to contain good fiber content (6.55%, 25 DV%), high quality of protein (3.12%, 6.24 DV%) containing: histidine, valine, threonine, leucine and lysine (with AAS 24.32, 23.66, 23.09, 23.05 and 21.71%, respectively), and 4.45% sugar that provides only 79 calories. Potassium was shown to be the abundant mineral content (793.43%, 22.66 DV), followed by copper and phosphorus (21.81 and 11.07 DV%, respectively). Sea buckthorn juice exhibited a rich phenolic, flavonoid and carotenoid content (283.58, 118.42 and 6.5 mg/g, respectively), in addition to a high content of vitamin C (322.33 mg/g). The HPLC profile indicated that benzoic acid is the dominant phenolic compound in sea buckthorn berries (3825.90 mg/kg). Antioxidant potentials (DPPH and ABTS) of sea buckthorn showed higher inhibition than ascorbic acid. Antimicrobial potentials were most pronounced against Escherichia coli BA12296 (17.46 mm). The probiotic growth was 8.5 log cfu/mL, with juice concentration, inoculum size and temperature as the main contributors to probiotic growth with a 95% confidence level. Fermentation of sea buckthorn juice with L. plantarum RM1 enhanced the functional phenolic and flavonoid content, as well as antioxidant and antimicrobial activities. The fermentation with L. plantarum RM1 enhanced the anti-hypertension and anticancer properties of the sea buckthorn juice and gained consumers’ sensorial overall acceptance.
Dietary Protein Modifies Hepatic Glycolipid Metabolism, Intestinal Immune Response, and Resistance to Streptococcus agalactiae of Genetically Improved Farmed Tilapia (GIFT: Oreochromis niloticus) Exposed to High Temperature
Authors: Huang Dongyu , Liang Hualiang , Zhu Jian , Ren Mingchun , Ge Xianping
Published: August 2022

The present study investigates the effects of dietary protein levels on glucolipid metabolism, immune function, and resistance to Streptococcus agalactiae of genetically improved farmed tilapia (GIFT) exposed to high temperature. Six practical diets were prepared to feed 360 fish (initial weight 43.78 ± 0.12 g) with graded protein levels (26.45%, 29.28%, 31.69%, 33.68%, 36.18%, and 38.75% dry matter). The results showed that 26.45% dietary protein significantly improved glycolysis by increasing PK mRNA levels, while the 29.28% and 31.69% dietary protein levels promoted gluconeogenesis by increasing PEPCK and G6Pase mRNA levels. For lipid metabolism, 26.45% dietary protein enhanced lipid synthesis by increasing PPAR-γ, SREBP1c, and FAS mRNA levels, while 31.69% dietary protein enhanced the level of lipolysis by increasing the PPAR-α and CPT1 mRNA levels. The highest plasma TG and TC contents were observed in the 29.28% and 31.69% dietary protein groups, respectively. In terms of antioxidants and immunity, the 31.69% dietary protein level activated the expression levels of HSP90 mRNA, thus increasing the expression levels of antioxidant-related genes (CAT, SOD, and GPx), and upregulating the anti-inflammatory factor IL-10 mRNA levels. In addition, regarding the antioxidant enzymes, the highest GSH content was found in the 29.28% dietary protein group, while the 31.69% dietary protein group had the maximum GSH-Px activity. The lowest plasma ALT and AST activities were observed in the 31.69% dietary protein group. Ultimately, the survival rate of juvenile GIFT fed 31.69% dietary protein was highest after a Streptococcus agalactiae challenge. Overall, 29.28–31.69% dietary protein was recommended in the diet of GIFT in a high-temperature environment.
Limits on the Rate of Conversion of Potential to Kinetic Energy in Quasigeostrophic Turbulence
Authors: Grooms Ian
Published: August 2022

Flow configurations that maximize the instantaneous rate of conversion from potential to kinetic energy are sought using a combination of analytical and numerical methods. A hydrostatic model is briefly investigated, but the presence of unrealistic ageostrophic flow configurations renders the results unrealistic. In the quasigeostrophic (QG) model, flow configurations that locally optimize the conversion rate are found, but it remains unclear if these flow configurations produce the global maximum conversion rate. The difficulty is associated with the fact that in the QG model, the vertical velocity is a quadratic function of the QG streamfunction, which renders the conversion rate a cubic function of the QG streamfunction. For these locally maximal conversion rates, the rate of conversion depends on the horizontal length scale of the flow: for scales larger than the deformation radius, the maximal rates are small and decrease as the horizontal scale increases; for scales smaller than the deformation radius, the maximal conversion rate rises until it becomes comparable to the maximal rate at which potential energy can be extracted from the mean flow.
Multi-Target Alternative Approaches to Promoting Fresh-Cut Carrots’ Bioactive and Fresh-like Quality
Authors: Alegria Carla , Gonçalves Elsa M. , Moldão-Martins Margarida , Abreu Marta
Published: August 2022

Fresh-cut fruits and vegetables, as near-fresh foods, are a quick and easy solution to a healthy and balanced diet. The rapid degradation of nutritional and sensory quality during the processing and storage of a product is critical and plant-type-dependent. The introduction of disruptive technological solutions in fresh-cut processing, which could maintain fresh-like quality with less environmental impact, is an emerging research concept. The application of abiotic stress treatments (heat shock and UV-C) induces metabolic responses and microbial effects in plant tissues, potentially slowing down several quality senescence pathways. The previously selected combined and single effects of heat shock (100 °C/45 s; in the whole root) and UV-C (2.5 kJ/m2) treatments and two packaging conditions (oriented polypropylene (OPP) vs. micro-perforated OPP films) on controlling critical degradation pathways of fresh-cut carrots and on promoting bioactive and sensory quality during storage (5 °C, 14 days) were studied. Among the tested combinations, synergistic effects on the quality retention of fresh-cut carrots were only attained for applying heat shock associated with micro-perforated OPP film packaging. Its effects on reducing (3.3 Log10 CFU/g) the initial contamination and controlling microbiological spoilage (counts below the threshold limit of 7.5 Log10 CFU/g), increasing the bioactive content (38% and 72% in total phenolic content and chlorogenic acid, respectively), and preserving fresh quality attributes prove to be a viable alternative technology for shredded carrot processing.
Manufacture of Whey Protein Hydrolysates Using Plant Enzymes: Effect of Processing Conditions and Simulated Gastrointestinal Digestion on Angiotensin-I-Converting Enzyme (ACE) Inhibitory Activity
Authors: Peslerbes Marie , Fellenberg Angélica , Jardin Julien , Deglaire Amélie , Ibáñez Rodrigo A.
Published: August 2022

Hydrolysis of proteins leads to the release of bioactive peptides with positive impact on human health. Peptides exhibiting antihypertensive properties (i.e., inhibition of angiotensin-I-converting enzyme) are commonly found in whey protein hydrolysates made with enzymes of animal, plant or microbial origin. However, bioactive properties can be influenced by processing conditions and gastrointestinal digestion. In this study, we evaluated the impact of three plant enzymes (papain, bromelain and ficin) in the manufacture of whey protein hydrolysates with varying level of pH, enzyme-to-substrate ratio and time of hydrolysis, based on a central composite design, to determine the degree of hydrolysis and antihypertensive properties. Hydrolysates made on laboratory scales showed great variation in the type of enzyme used, their concentrations and the pH level of hydrolysis. However, low degrees of hydrolysis in papain and bromelain treatments were associated with increased antihypertensive properties, when compared to ficin. Simulated gastrointestinal digestion performed for selected hydrolysates showed an increase in antihypertensive properties of hydrolysates made with papain and bromelain, which was probably caused by further release of peptides. Several peptides with reported antihypertensive properties were found in all treatments. These results suggest plant enzymes used in this study can be suitable candidates to develop ingredients with bioactive properties.
Insight into the Influence of Lactic Acid Bacteria Fermentation on the Variations in Flavor of Chickpea Milk
Authors: Zhang Xue , Tian Wenli , Xie Bijun , Sun Zhida
Published: August 2022

This study aimed to evaluate the influence of fermentation on the levels of free amino acids (FAAs) and variations of volatile odorants in four groups of chickpea milk. Electronic nose (E-nose) and gas chromatography–mass spectrometry (GC-MS) data were subjected to mutual validation. W2S and W3S sensors of E-nose were sensitive to volatile constituents in the four groups of unfermented and fermented specimens. After fermentation, the levels of FAAs in the four groups of specimens decreased to varying degrees. Additionally, there were remarkable differences in the types and contents of volatile odor substances in all specimens before and after fermentation. The principal component analysis findings based on E-nose identified the changes of volatile odorants in all specimens before and after fermentation. GC-MS identified 35 and 55 volatile flavor substances in unfermented and fermented specimens, respectively. The varieties of volatile odor substances in fermented chickpea milk (FCM) with papain treatment plus yam addition (38) were more than those in FCM (24), indicating that the coupled treatment of enzymolysis and yam addition could enrich the volatile odorants in fermented specimens. After probiotic fermentation, the contents of off-flavor substances decreased to a certain extent, and key aroma substances such as 2,3-pentanedione, 2,3-butanedione, and heptyl formate were detected. These results demonstrated that lactic acid bacterial fermentation on the basis of enzymolysis and yam addition could be utilized as a feasible approach to improve the flavor of plant-based products adopting chickpea as the original ingredient.
Elucidation of the Flavor Aspects and Flavor-Associated Genomic Regions in Bottle Gourd (Lagenaria siceraria) by Metabolomic Analysis and QTL-seq
Authors: Wang Ying , Li Yanwei , Wu Xiaohua , Wu Xinyi , Feng Zishan , Wang Jian , Wang Baogen , Lu Zhongfu , Li Guojing
Published: August 2022

Bottle gourd (Lagenaria siceraria) is a commercially important cucurbitaceous vegetable with health-promoting properties whose collections and cultivars differ considerably in their flavor aspects. However, the metabolomic profile related to flavor has not yet been elucidated. In the present study, a comprehensive metabolite analysis revealed the metabolite profile of the strong-flavor collection “J120” and weak-flavor collection “G32”. The major differentially expressed metabolites included carboxylic acids, their derivatives, and organooxygen compounds, which are involved in amino acid biosynthesis and metabolism. QTL-seq was used to identify candidate genomic regions controlling flavor in a MAGIC population comprising 377 elite lines. Three significant genomic regions were identified, and candidate genes likely associated with flavor were screened. Our study provides useful information for understanding the metabolic causes of flavor variation among bottle gourd collections and cultivars. Furthermore, the identified candidate genomic regions may facilitate rational breeding programs to improve bottle gourd quality.
An Amateur-Radio-Based Open-Source (HW/SW) VLF/LF Receiver for Lower Ionosphere Monitoring, Examples of Identified Perturbations
Authors: Malkotsis Filopimin , Politis Dimitrios Z. , Dimakos Dionisis , Potirakis Stelios M.
Published: August 2022

The ground-based monitoring of the lower ionosphere by studying the perturbations of the subionospheric propagation of very-low-frequency/low-frequency (VLF/LF) signals is important in the research of a wide variety of geophysical and Sun/space extreme phenomena. Such perturbations are identified as anomalies in the signal received from the VLF/LF transmitters operating worldwide for military purposes, time code broadcasting, etc. Especially for the study of local ionosphere-influencing phenomena, such as earthquakes, volcanoes, typhoons, etc., the monitoring of several subionospheric propagation paths is necessary. However, it is very difficult to find in the market (or reproduce) hardware (HW) for wide-band VLF/LF receivers that could receive many different transmitters, while the involved software (SW) is mainly proprietary. Aiming to provide a low-cost and easy-to-build alternative for the scientists involved in this research field, we suggest a VLF/LF receiver setup based on amateur radio open-source HW and SW. Its key components are the so-called “mini-whip” active antenna and the freeware “SpectrumLab” and “GPS2Time”. The full HW schematics and all settings of the employed SW configuration for the proposed VLF/LF receiver setup are provided in the article. To check the reliability of the proposed receiver setup, two almost identical VLF/LF radio receivers were installed in the prefecture of Attica in Greece, in June and September of 2021, respectively. Examples of ionospheric perturbations due to different phenomena (solar flares, earthquakes, and a magnetic storm) are provided to show the ability of the proposed receiver setup to provide reliable data for ionosphere-related research.
Operating Room Usage Time Estimation with Machine Learning Models
Authors: Chu Justin , Hsieh Chung-Ho , Shih Yi-Nuo , Wu Chia-Chun , Singaravelan Anandakumar , Hung Lun-Ping , Hsu Jia-Lien
Published: August 2022

Effectively handling the limited number of surgery operating rooms equipped with expensive equipment is a challenging task for hospital management such as reducing the case-time duration and reducing idle time. Improving the efficiency of operating room usage via reducing the idle time with better scheduling would rely on accurate estimation of surgery duration. Our model can achieve a good prediction result on surgery duration with a dozen of features. We have found the result of our best performing department-specific XGBoost model with the values 31.6 min, 18.71 min, 0.71, 28% and 27% for the metrics of root-mean-square error (RMSE), mean absolute error (MAE), coefficient of determination (R2), mean absolute percentage error (MAPE) and proportion of estimated result within 10% variation, respectively. We have presented each department-specific result with our estimated results between 5 and 10 min deviation would be more informative to the users in the real application. Our study shows comparable performance with previous studies, and the machine learning methods use fewer features that are better suited for universal usability.
Osteopathic Treatment for Gastrointestinal Disorders in Term and Preterm Infants: A Systematic Review and Meta-Analysis
Authors: Buffone Francesca , Monacis Domenico , Tarantino Andrea Gianmaria , Dal Farra Fulvio , Bergna Andrea , Agosti Massimo , Vismara Luca
Published: August 2022

The aim of this systematic review and meta-analysis is to evaluate the effectiveness of osteopathic manipulative treatment (OMT) for gastrointestinal disorders in term and preterm infants. Eligible studies were searched on PubMed, Scopus, Embase, Cochrane, Cinahl, and PEDro. Two reviewers independently assessed if the studies were randomized controlled trials (RCTs) and retrospective studies with OMT compared with any kind of control in term or preterm infants to improve gastrointestinal disorders. Nine articles met the eligibility criteria, investigating OMT compared with no intervention, five involving term infants, and the remaining treating preterm infants. Five studies showed low risk of bias. In the meta-analysis, two studies were included to analyze the hours of crying due to infantile colic, showing statistically significant results (ES = −2.46 [−3.05, −1.87]; p < 0.00001). The quality of evidence was “moderate”. The other outcomes, such as time to oral feeding, meconium excretion, weight gain, and sucking, were presented in a qualitative synthesis. OMT was substantially safe, and showed efficacy in some cases, but the conflicting evidence and lack of high-quality replication studies prevent generalization. High-quality RCTs are recommended to produce better-quality evidence.
Effects of Plasticulture and Conservation Tillage on Nematode Assemblage and Their Relationships with Nitrous Oxide Emission following a Winter Cover Cropping and Vegetable Production System
Authors: Wang Koon-Hui , Waisen Philip , Paudel Roshan , Chen Guihua , Meyer Susan Lynn Fricke , Hooks Cerruti R. R.
Published: August 2022

Agriculture production emits significant amounts of nitrous oxide (N2O), a greenhouse gas with high global warming potential. The objectives of this study were to examine whether different husbandry practices (tillage and plasticulture) following winter cover cropping would influence soil food web structure and whether a change in the soil community could help mitigate N2O emission in vegetable plantings. Three consecutive field trials were conducted. A winter cover crop mix of forage radish (Raphanus sativus), crimson clover (Trifolium incarnatum) and cereal rye (Secale cereale) were planted in all plots. Winter cover crop was terminated by flail mowing followed by (1) conventional till without surface residues [Bare Ground (BG)], (2) conventional till with black plastic mulch (BP) without surface residues, (3) strip-till (ST) with partial surface residues, or (4) no-till (NT) with surface residues. The cash crop planted subsequently were eggplant (Solanum melongena) in 2012 and 2014 and sweet corn (Zea mays) in 2013. The soil food web structure was consistently disturbed in the BP compared to other treatments as indicated by a reduction in the abundance of predatory nematodes in 2012 and 2014, and nematode maturity index in 2013 in BP. Changes in soil food web structure in the conservation tillage (NT or ST) treatments based on the weight abundance of nematode community analysis were not consistent and did not improve over the 3-year study; but were consistently improved based on functional metabolic footprint calculation at termination of cover crops of 2013 and 2014. None-the-less, the N2O emissions increased as the abundance of fungivorous nematodes increased during all three trials. It was also found that improved soil food web structure [higher abundance of omnivorous in 2012 or predatory nematodes in 2013 and 2014, and structure index (SI) in all 3 years] reduced N2O emissions. These findings suggested that proper soil husbandry practices following winter cover cropping could mitigate N2O emissions over time.
Emerging Technologies for Prolonging Fresh-Cut Fruits’ Quality and Safety during Storage
Authors: Iturralde-García Rey David , Cinco-Moroyoqui Francisco Javier , Martínez-Cruz Oliviert , Ruiz-Cruz Saúl , Wong-Corral Francisco Javier , Borboa-Flores Jesús , Cornejo-Ramírez Yaeel Isbeth , Bernal-Mercado Ariadna Thalia , Del-Toro-Sánchez Carmen Lizette
Published: August 2022

Fresh-cut fruits have been in great demand by consumers owing to the convenience of buying them in shopping centers as ready-to-eat products, and various advantages, such as the fact that they are healthy and fresh products. However, their shelf lives are brief due to their physiological changes and maturation. Therefore, this review includes information from the physicochemical, microbiological, nutritional, and sensory points of view on the deterioration mechanisms of fresh-cut fruits. In addition, updated information is presented on the different emerging technologies, such as active packaging (edible films, coatings, and modified atmospheres), natural preservatives (antioxidants and antimicrobials), and physical treatments (high hydrostatic pressure, UV-C radiation, and ozone). The benefits and disadvantages of each of these technologies and the ease of their applications are discussed. Having alternatives to preserve fresh-cut fruit is essential both for the consumer and the merchant, since the consumer could then obtain a high-quality product maintaining all its properties without causing any damage, and the merchant would receive economic benefits by having more time to sell the product.
The Impact of Mobile Phone Reminders on Perceived Self-Care Levels of Informal Caregivers
Authors: Peres Beatriz , Gouveia Élvio Rúbio , Campos Pedro F.
Published: August 2022

Informal caregivers play a fundamental role in caring for people that need assistance and provide an effective service in managing their loved ones’ health. Because of this, they have little time to attend to themselves and perform self-care practices. Some of these practices can improve mental health. By snowball sampling, we recruited 15 informal caregivers, 12 females and three males. Using the Wilcoxon signed-rank test, we analyzed the impact of the reminders on a self-care scale. We used the Mindful Self-Care Scale, comparing the same population without reminders and with reminders. Results indicated that total self-care scores with reminders increased statistically significantly, T = 13, Z = −2.481, p ≤ 0.013, with a large effect size (r = 0.64). This study shows a significant self-care increase in informal caregivers after using reminders. Future development of a reminders-based approach could focus on increasing self-care and the time caregivers take for themselves, empowering them to take a more active role in meeting their own needs.
Energy Levels in Pentacoordinate d5 to d9 Complexes
Authors: Titiš Ján , Rajnák Cyril , Boča Roman
Published: August 2022

Energy levels of pentacoordinate d5 to d9 complexes were evaluated according to the generalized crystal field theory at three levels of sophistication for two limiting cases of pentacoordination: trigonal bipyramid and tetragonal pyramid. The electronic crystal field terms involve the interelectron repulsion and the crystal field potential; crystal field multiplets account for the spin–orbit interaction; and magnetic energy levels involve the orbital– and spin–Zeeman interactions with the magnetic field. The crystal field terms are labelled according to the irreducible representations of point groups D3h and C4v using Mulliken notation. The crystal field multiplets are labelled with the Bethe notations for the respective double groups D’3 and C’4. The magnetic functions, such as the temperature dependence of the effective magnetic moment and the field dependence of the magnetization, are evaluated by employing the apparatus of statistical thermodynamics as derivatives of the field-dependent partition function. When appropriate, the formalism of the spin Hamiltonian is applied, giving rise to a set of magnetic parameters, such as the zero-field splitting D and E, magnetogyric ratio tensor, and temperature-independent paramagnetism. The data calculated using GCFT were compared with the ab initio calculations at the CASSCF+NEVPT2 level and those involving the spin–orbit interaction.
Virtual Reality Greenspaces: Does Level of Immersion Affect Directed Attention Restoration in VR Environments?
Authors: Dillon Denise , Cai Jiaying
Published: August 2022

Research on attention restoration theory has provided consistent results over the years, and exposure to nature has been found to significantly improve and restore directed attention fatigue. However, rapid urbanisation has made it increasingly difficult for human beings to interact with unpolluted nature. Countries such as Singapore began to integrate nature into city planning and into the designs of buildings to help resident satisfaction and well-being. Local studies have had contradictory outcomes; residents report significantly higher restoration and affect towards vertical integrated greeneries, but many would still prefer traditional on-the-ground foliage. Interest in outdoor greenspaces is variable because Singapore’s high heat and humid climate make it uncomfortable to interact with outside nature for prolonged periods. The current study tested alternative modalities that can provide residents with on-the-ground foliage interactions, without the discomfort of being outdoors. Virtual exposures with different levels of immersion (high and low) and types of scenes (nature and urban) were tested for effects on perceived presence, perceived restoration and objective restoration. A total of 120 adult participants completed the study, which revealed that a virtual reality-nature condition provided significantly superior directed attention restoration and higher presence ratings. Immersive tendency did not affect perceived presence; neither did it moderate the relationship between perceived presence and its corresponding perceived restoration.
The Coadministration of Levosimendan and Exenatide Offers a Significant Cardioprotective Effect to Isolated Rat Hearts against Ischemia/Reperfusion Injury
Authors: Leivaditis Vasileios , Koletsis Efstratios , Tsopanoglou Nikolaos , Charokopos Nikolaos , D’Alessandro Cristian , Grapatsas Konstantinos , Apostolakis Efstratios , Choleva Effrosyni , Plota Maria , Emmanuil Andreas , Dahm Manfred , Dougenis Dimitrios
Published: August 2022

(1) Background: The present study aims to investigate the effect of administration of Levosimendan and Exenatide in various concentrations, as well as of the coadministration of those agents in an ischemia–reperfusion injury isolated heart model. (2) Methods: After 30 min of perfusion, the hearts underwent a 30 min period of regional ischemia followed by a 120 min period of reperfusion. All animals were randomly divided into 12 experimental groups of nine animals in each group: (1) Control, (2) Sham, (3) Digox (Negative control, Digoxin 1.67 μg/min), (4) Levo 1 (Levosimendan 0.01 μg/min), (5) Levo 2 (Levosimendan 0.03 μg/mL), (6) Levo 3 (Levosimendan 0.1 μg/min), (7) Levo 4 (Levosimendan 0.3 μg/min), (8) Levo 5 (Levosimendan 1 μg/min), (9) Exen 1 (Exenatide 0.001 μg/min), (10) Exen 2 (Exenatide 0.01 μg/min), (11) Exen 3 (Exenatide 0.1 μg/min) and (12) Combi (Levosimendan 0.1 µg/mL + Exenatide 0.001 μg/min). The hemodynamic parameters were recorded throughout the experiment. Arrhythmias and coronary flow were also evaluated. After every experiment the heart was suitably prepared and infarct size was measured. Markers of myocardial injury were also measured. Finally, oxidative stress was evaluated measuring reactive oxygen species. (3) Results: A dose-dependent improvement of the haemodynamic response was observed after the administration of both Levosimendan and Exenatide. The coadministration of both agents presented an even greater effect, improving the haemodynamic parameters further than the two agents separately. Levosimendan offered an increase of the coronary flow and both agents offered a reduction of arrhythmias. A dose-dependent reduction of the size of myocardial infarction and myocardial injury was observed after administration of Levosimendan and Exenatide. The coadministration of both agents offered a further improving the above parameters. Levosimendan also offered a significant reduction of oxidative stress. (4) Conclusions: The administration of Levosimendan and Exenatide offers a significant benefit by improving the haemodynamic response, increasing the coronary flow and reducing the occurrence of arrhythmias, the size of myocardial injury and myocardial oxidative stress in isolated rat hearts.
Drone Model Classification Using Convolutional Neural Network Trained on Synthetic Data
Authors: Wisniewski Mariusz , Rana Zeeshan A. , Petrunin Ivan
Published: August 2022

We present a convolutional neural network (CNN) that identifies drone models in real-life videos. The neural network is trained on synthetic images and tested on a real-life dataset of drone videos. To create the training and validation datasets, we show a method of generating synthetic drone images. Domain randomization is used to vary the simulation parameters such as model textures, background images, and orientation. Three common drone models are classified: DJI Phantom, DJI Mavic, and DJI Inspire. To test the performance of the neural network model, Anti-UAV, a real-life dataset of flying drones is used. The proposed method reduces the time-cost associated with manually labelling drones, and we prove that it is transferable to real-life videos. The CNN achieves an overall accuracy of 92.4%, a precision of 88.8%, a recall of 88.6%, and an f1 score of 88.7% when tested on the real-life dataset.