bluuebunny/crossref_metadata_2025 huggingface dataset:
Analyzing Characteristics of Grassland Gross Ecosystem Product to Inform Decision Making in the Karst Desertification Control
Authors: Li Yongyao , Xiong Kangning , Zhang Wenfang , Song Shuzhen , Luo Lu
Published: July 2023

Synergistically enhancing and realizing the value of grassland ecosystem services (ES) for economic activity is an important but challenging task for achieving sustainability in the karst desertification control (KDC). However, how to use grassland ES value characteristics in the KDC to make decisions on ES improvement, human well-being enhancement, and sustainable development remains unclear. In this paper, we took the contiguous region of karst desertification in Yunnan-Guangxi-Guizhou, China, a global hotspot, as the study area. Based on the valuation of the gross ecosystem product (GEP) and county economic intensity, we analyzed the structural and spatial characteristics of grassland GEP in the KDC using spatial analysis methods. We found that: (1) the grassland GEP in the KDC is mainly distributed in counties with low economic intensity (86.05% of the total number of counties) and vulnerable to losses caused by the livelihood of farmers; (2) the grassland GEP in the KDC is spatially small and scattered (the geographic concentration lies between 0.015 and 0.237), which makes it difficult to form industrial scale advantages; (3) the public product index (66.22–96.77%) and industry scale concentration (97.87–99.86%) of grassland GEP in the KDC are high, and most of the GEP is difficult to transform on the private market. Based on our findings, we proposed three corresponding recommendations for economic decision-making. The results of this study can provide a reference for economic decision-making regarding the management of grassland ES in karst areas with similar conditions and beyond.
Herbicide Resistance in Summer Annual Weeds of Australia’s Northern Grains Region
Authors: Broster John C. , Jalaludin Adam , Widderick Michael J. , Chambers Allison J. , Walsh Michael J.
Published: July 2023

For more than two decades, glyphosate has been relied on to control summer annual weeds in fallow systems in Australia’s northern grains region. With numerous cases of glyphosate resistance reported in weed species collected from this region, there are concerns about the future viability of this herbicide. A random seed collection survey of summer weeds was conducted between 2016 and 2018 with the aim of determining the frequency and distribution of resistance to glyphosate and other herbicides commonly used for summer weed control. Glyphosate resistance was ubiquitous in fleabane, with all collected populations resistant to this herbicide. Glyphosate resistance was also prevalent in feathertop Rhodes grass, windmill grass, and awnless barnyard grass, with resistance detected in 68%, 58%, and 36% of populations, respectively. Only 14% of sowthistle populations collected between 2013 and 2108 were resistant to glyphosate. Resistance to haloxyfop was detected in feathertop Rhodes grass, albeit at a low frequency (2%). Other herbicides, such as 2,4-D amine, propaquizafop, and clethodim, provided good control of the broadleaf and grass weeds tested. The results from these surveys conducted between 2013 and 2017 provide a first glimpse of the state of herbicide resistance in key crop weeds for Queensland and the northern region of New South Wales. It is clear that farmers and agronomists need to consider incorporating non-chemical weed management tactics to promote the sustainability of current herbicides.
Edamame Yield and Quality Response to Nitrogen and Sulfur Fertilizers
Authors: Brooks Keren , Reiter Mark , Zhang Bo , Mott Joshua
Published: July 2023

As United States farmers adapt soybean (Glycine max) production methods from oilseed to vegetable (edamame), key management practices will need to be considered. The key objective of this study was to determine the optimal nitrogen (N) rate and N application timing for edamame in the mid-Atlantic coastal plain system. The study was conducted for three years in Painter, VA, USA on sandy loam soils. A factorial arrangement of four N rates was applied with two application timing strategies: at-planting, and split application. Leaf tissue samples were collected and analyzed at R1. At harvest, the Normalized Difference Vegetation Index (NDVI) was measured, whole pods were mechanically collected, and yield was recorded. Additionally, pod and bean physical and chemical quality were assessed. Nitrogen fertilization significantly increased pod yield in two out of three years. R1 leaf N and sulfur (S) concentrations correlated to the yield, and R1 leaf and R6 whole-plant N concentrations correlated to the total N uptake. None of the tested parameters indicated that N fertilizer decreased yield or quality. In conclusion, we found that N fertilizer applied at planting may aid edamame yield and profit for sandy loam soils in the mid-Atlantic, USA.
Effects of the Changes of Particle Surface Electric Field and Interaction Force on the Reclaimed Soil Aggregate Structural Stability under the Application of Different Soil Conditioners
Authors: Liu Zhe , Zhang Yang , Sun Yingying , Han Jichang , Hu Feinan , Li Junchao , Li Xuxiang
Published: July 2023

Aggregate stability is a key factor in the evaluation of soil structure and erosion resistance, which is largely influenced by soil electric field and particle interaction. However, there are few studies on how different organic and inorganic soil conditioners change the surface electric field and interaction force of reclaimed soil to improve the aggregate stability. Therefore, a five-year field experiment was conducted to quantitatively study the effects of FeSO4 (TM), organic fertilizer (TO), fly ash (TF), maturing agent + organic fertilizer (TMO) and fly ash + organic fertilizer (TFO), compared with control (CK) treatment, on the reclaimed soil internal force and the aggregate crushing strength. The results showed that the reclaimed soil surface potential and electric field intensity increased after 5 years of application of organic and inorganic soil conditioners. Under the same electrolyte concentration and electric field conditions, the crushing strength of aggregates (<5 μm) treated with TFO, TMO, TO, TF and TM decreased by 43.70%, 35.51%, 25.97%, 8.28% and 5.49%, respectively, compared with the control treatment, and the combination of organic and inorganic treatment (TFO and TMO) had a better effect on improving the aggregate crushing resistance. With the application of soil conditioners, the reclaimed soil DLVO force and net resultant force gradually decreased, and the order of magnitude was TFO < TMO < TO < TF < TM < CK, indicating that the application of organic and inorganic soil conditioners enhanced the van der Waals attractive force and net attractive force between reclaimed soil particles, and reduced the net repulsive force between particles. The theoretical calculation results of the reclaimed soil internal force well explain the experimental results of aggregate stability against crushing, and the relationship between aggregate crushing strength and net resultant force is exponential (p < 0.01). Generally speaking, the soil conditioners increase the net attractive force between particles, reduce the possibility of violent crushing of aggregates due to the increase of electric field intensity and improve the aggregate structural stability, among which the combined application of organic and inorganic soil conditioners has a better improvement effect. The results of this study will lay a theoretical foundation for clarifying the improvement of different soil conditioners on the reclaimed soil structural stability and erosion resistance.
The Role of Preventative Medicine Programs in Animal Welfare and Wellbeing in Zoological Institutions
Authors: Martelli Paolo , Krishnasamy Karthiyani
Published: July 2023

The overarching goal of a preventative medicine program is to minimize the chances of health problems developing and to maximize the chances of detecting health problems early, in a manner that best benefits the animals and the organization. The traditional paradigms of animal welfare, stemming from the five freedoms and being progressively fleshed out to five domains, the 24/7 approach and so forth do not apply perfectly to zoological collections and less so to animals undergoing veterinary treatments. The physiology and behaviour of animals undergoing veterinary treatments, including therapeutic, quarantine and preventative medicine, are derailed from their normal states and their choices and comfort are de facto limited. A paradigm separating animal wellbeing from animal welfare is necessary to instil clarity of thought and to guide actions in regard to the welfare of animals under human care. Using such a model, preventative medicine programs emerge as a cornerstone of zoo and aquarium animal welfare, all the more if it incorporates modern veterinary and husbandry techniques, including operant conditioning.
Non-Invasive Biomarkers in Saliva and Eye Infrared Thermography to Assess the Stress Response of Calves during Transport
Authors: Lei Mariana Caipira , Félix Luís , Cardoso Ricardo , Monteiro Sandra Mariza , Silva Severiano , Venâncio Carlos
Published: July 2023

Animal transport is currently a stressful procedure. Therefore, animal-based indicators are needed for reliable and non-invasive welfare assessment. Saliva is a biospecimen with potential validity for the determination of cortisol and oxidative stress, although its use to assess calf welfare during transport has never been tested. Similarly, the applicability and reliability of infrared thermography to assess temperature change during calves’ transport have never been evaluated. These objectives were outlined following the known and growing need to identify non-invasive methodologies for stress assessment in bovines. This study was conducted on 20 calves of the Arouquesa autochthone breed, at about nine months of age, during their transport to slaughter. For each animal, saliva samples and thermographic images of the eye were collected at three time points: before transport, after transport, and at slaughter. The saliva was then processed to measure cortisol levels and oxidative stress parameters (reactive oxygen species, thiobarbituric acid reactive substance, carbonyls, and advanced oxidation protein products), and the images were analyzed using FLIR Tools+ software. There was an increase in cortisol concentration and oxidative stress parameters (reactive oxygen species, thiobarbituric acid reactive substance, carbonyls, and advanced oxidation protein products) in saliva after transport. An increase in eye temperature triggered by transport was also observed. The cortisol and eye temperature results at slaughter were returned to values similar to those before transport; however, the values of oxidative stress remained increased (mainly TBARS values). These non-invasive techniques seem to be reliable indicators of stress in bovine transport, and oxidative stress parameters in saliva may be a persistent marker for welfare assessment.
Phylogenetic Analysis of G and P Genotypes of Bovine Group A Rotavirus Strains Isolated from Diarrheic Vietnam Cows in 2017 and 2018
Authors: Shin Jihye , Park Gyu-Nam , Choe SeEun , Cha Ra Mi , Kim Ki-Sun , An Byung-Hyun , Kim Song Yi , Moon Soo Hyun , Hyun Bang-Hun , An Dong-Jun
Published: July 2023

This study aimed to investigate the genetic diversity of G- and P-type bovine RVAs (BoRVAs) prevalent in Vietnam. Between 2017 and 2018, the prevalence of BoRVAs detected in diarrhea samples from 8 regions was as low as 1.9% (11/582). The prevalence of the G-type was 45.5% for G6 and 18.2% for G10; however, 36.3% remain unidentified. Interestingly, all BoRVAs were investigated as P[11], and there was no diversity within this P-type. Geographically, the G6 and G10 types were not identified in any specific area; rather, they occurred in both Northern and Southern Vietnam. G6P[11] and G10P[11], which are combined G- and P-types, were identified in 71.4% and 28.6% of BoRVA-positive samples, respectively. Phylogenetic tree analysis revealed that the G6-type detected in Vietnamese cows is similar to strains derived from China, Japan, and Korea, whereas the G10 type is closely related to the Chinese strain. In addition, the P11 strain detected in Vietnamese cows is similar to the Spanish and Chinese strains. The BoRVA-positive rate was higher in cows aged less than 2 months (3.2%, 3/94) than in those aged 2 months or more (1.6%, 8/488). In summary, we detected the presence of G6P11 and G10P11 BoVRAs on Vietnamese cow farms, and found that they were more predominant in young calves than in older cows.
Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene CKM and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts
Authors: Chen Bingjie , Wang Yanxing , Hou Dan , Zhang Yushi , Zhang Bochun , Niu Yufang , Ji Haigang , Tian Yadong , Liu Xiaojun , Kang Xiangtao , Cai Hanfang , Li Zhuanjian
Published: July 2023

Skeletal muscle is an essential tissue in meat-producing animals, and meat-producing traits have been a hot topic in chicken genetic breeding research. Current research shows that creatine kinase M-type-like (CKM) is one of the most abundant proteins in skeletal muscle and plays an important role in the growth and development of skeletal muscle, but its role in the development of chicken skeletal muscle is still unclear. Via RNA sequencing (RNA-seq), we found that CKM was highly expressed in chicken breast muscle tissue. In this study, the expression profile of CKM was examined by quantitative real-time PCR (qPCR), and overexpression and RNA interference techniques were used to explore the functions of CKM in the proliferation, apoptosis and differentiation of chicken primary myoblasts (CPMs). It was shown that CKM was specifically highly expressed in breast muscle and leg muscle and was highly expressed in stage 16 embryonic muscle, while CKM inhibited proliferation, promoted the apoptosis and differentiation of CPMs and was involved in regulating chicken myogenesis. Transcriptome sequencing was used to identify genes that were differentially expressed in CPMs after CKM disruption, and bioinformatics analysis showed that CKM was involved in regulating chicken myogenesis. In summary, CKM plays an important role in skeletal muscle development during chicken growth and development.
The Prevalence and Risk Factors of Acute Kidney Injury during Colistin Therapy: A Retrospective Cohort Study from Lebanon
Authors: Moghnieh Rima , Husni Rola , Helou Mariana , Abdallah Dania , Sinno Loubna , Jadayel Marwa , Diab Kawsar , Chami Carmen , Al Rachid Marah , Awad Diana Caroline , Zaiter Aline , Sayegh Mohamed H.
Published: July 2023

Introduction: The current study aimed to determine the prevalence, risk factors, and stages of severity of acute kidney injury (AKI) caused by colistimethate sodium (CMS) treatment in patients diagnosed with systemic antibiotic-resistant Gram-negative bacterial infections. The predictors of all-cause mortality in this patient population were also examined. Methods: This retrospective cohort study included patients who were admitted to a university-affiliated hospital and acute tertiary care referral center in Beirut, Lebanon between January 2015 and December 2018 and underwent CMS treatment for a period of 48 h or more. Results: The study sample included 298 adult patients, of which 46.3% (n = 138/298) developed AKI (assessed using the Kidney Disease Improving Global Outcomes (KDIGO) criteria). Of these, 37.7% (n = 51/138) were diagnosed with stage 1 AKI, 23.9% with stage 2 (n = 33/138), and 38.4% with stage 3 (n = 53/138). Nephrotoxicity was reversed in 87.5% of AKI patients who survived until hospital discharge. Independent risk factors for AKI included patient age ≥ 75 years (aOR = 1.854; 95% CI: 1.060–3.241; p-value = 0.03); underlying chronic kidney disease (aOR = 4.849; 95% CI: 2.618–9.264; p-value < 0.0001); and concomitant use of vasopressors (aOR = 4.305; 95% CI: 2.517–7.456; p-value < 0.0001). Multivariate analysis showed that the predictors of severe AKI (stage 2 or 3) included baseline hypoalbuminemia (aOR = 2.542; 95% CI: 1.000–6.564; p-value = 0.05); concomitant use of vasopressors (aOR = 6.396; 95% CI: 2.741–15.87; p-value < 0.0001); and CMS days of therapy (DOT) prior to development of AKI ≥ 7 days (aOR = 4.728; 95% CI: 2.069–11.60; p-value < 0.0001). All-cause mortality was recorded in 51.3% of patients (n = 153/298), and this was significantly higher in patients with AKI (76.8%; n = 106/138) compared to those without (29.4%; n = 47/160; OR = 7.964; 95% CI: 4.727–13.417; p-value < 0.0001). Independent predictors of all-cause mortality included a baseline Charlson comorbidity index score ≥5 (aOR = 4.514; 95% CI: 2.443–8.530; p-value < 0.0001); concomitant use of vasopressors (aOR = 7.76; 95% CI: 4.238–14.56; p-value < 0.0001); and CMS-induced AKI (aOR = 4.117; 95% CI: 2.231–7.695; p-value < 0.0001). Conclusions: The findings of this study suggest that old age, history of chronic kidney disease, and concomitant vasopressor treatment are all independent predictors of CMS-induced AKI. The risk of developing severe AKI significantly increases with CMS DOT. Understanding the risk factors of nephrotoxicity is essential for improving prognosis and treatment outcomes.
Comparative Transcriptomic Profiling of Pellicle and Planktonic Cells from Carbapenem-Resistant Acinetobacter baumannii
Authors: Ng Heng Kang , Puah Suat Moi , Teh Cindy Shuan Ju , Idris Nuryana , Chua Kek Heng
Published: July 2023

Acinetobacter baumannii forms air–liquid interface pellicles that boost its ability to withstand desiccation and increase survival under antibiotic pressure. This study aims to delve into the transcriptomic profiles of pellicle cells from clinical strains of carbapenem-resistant A. baumannii (CRAB). The total RNA was extracted from pellicle cells from three pellicle-forming CRAB strains and planktonic cells from three non-pellicle-forming CRAB strains, subject to RNA sequencing using Illumina HiSeq 2500 system. A transcriptomic analysis between pellicle and planktonic cells, along with differential expression genes (DEGs) analysis and enrichment analysis of annotated COGs, GOs, and KEGGs, was performed. Our analysis identified 366 DEGs in pellicle cells: 162 upregulated genes and 204 downregulated genes. The upregulated ABUW_1624 (yiaY) gene and downregulated ABUW_1550 gene indicated potential involvement in fatty acid degradation during pellicle formation. Another upregulated ABUW_2820 (metQ) gene, encoding the D-methionine transporter system, hinted at its contribution to pellicle formation. The upregulation of two-component systems, CusSR and KdpDE, which implies the regulation of copper and potassium ions in a CRAB pellicle formation was also observed. These findings provide valuable insights into the regulation of gene expression during the formation of pellicles in CRAB, and these are potential targets that may aid in the eradication of CRAB infections.
Effect of N-Acetyl-L-cysteine on Activity of Doxycycline against Biofilm-Forming Bacterial Strains
Authors: Petkova Tsvetelina , Rusenova Nikolina , Danova Svetla , Milanova Aneliya
Published: July 2023

Biofilm-forming bacteria are associated with difficult-to-cure bacterial infections in veterinary patients. According to previous studies, N-acetyl-L-cysteine (NAC) showed an inhibitory effect on biofilm formation when it was applied in combination with beta-lactam antibiotics and fluoroquinolones. The lack of information about the effect of NAC on doxycycline activity against biofilm-forming strains was the reason for conducting this study. Staphylococcus aureus (S. aureus) ATCC 25923, Staphylococcus aureus O74, Escherichia coli (E. coli) ATCC 25922 and Pseudomonas aeruginosa (P. aeruginosa) ATCC 27853 were used to evaluate the activity of doxycycline with and without addition of NAC on planktonic bacteria and on biofilm formation. The minimum inhibitory concentrations (MICs) of doxycycline were not affected by NAC for Gram-negative strains and were found to be two times higher for the strains of S. aureus. The minimum biofilm inhibitory concentrations (MBICs) for Gram-negative bacteria (2 μg/mL for E. coli ATCC 25922 and 32 μg/mL for P. aeruginosa ATCC 27853), determined using a standard safranin colorimetric assay, were higher than the MICs (0.5 and 4 μg/mL, respectively). The data suggest that the combinations of doxycycline and NAC could stimulate the growth of planktonic cells of S. aureus and biofilm-forming E. coli ATCC 25922. NAC did not affect the strong inhibitory effect of doxycycline on the biofilm formation by the strains of S. aureus.
Use of Winemaking By-Products for the Functionalization of Polylactic Acid for Biomedical Applications
Authors: Verano-Naranjo Lidia , Cejudo-Bastante Cristina , Casas Lourdes , Martínez de la Ossa Enrique , Mantell Casimiro
Published: July 2023

The addition of naturally active compounds to implantable polymers is an efficient strategy against inflammation issues that might lead to rejection, while promoting controlled re-endothelialization of the tissues. This work proposes the use of winemaking by-products with high active properties of biomedical interest to obtain bioactive PLA by using supercritical technologies. First, two red grape pomace extracts, obtained by high-pressure extraction with supercritical CO2 and cosolvents (either ethanol or water–ethanol), have been studied. Second, two impregnation methods have been studied with both extracts, traditional supercritical CO2-assisted impregnation (SSI) and a novel pressurized soaking method (PSI). The amount of extract impregnated as well as the bioactivity levels achieved—i.e., antioxidant, antimicrobial, and anti-inflammatory properties— have been determined for each extract and impregnation method at different pressure and temperature conditions. Both extracts obtained had good antioxidant, anti-inflammatory, and antibacterial capacities, especially the hydroethanolic one (0.50 ± 0.03 mg TE/g versus 0.24 ± 0.03 mg TE/g, respectively). Regarding impregnated filaments, impregnation loadings depended especially on the extract and P/T conditions, providing up to 8% (extract mass/polymer mass) of impregnation. The antioxidant capacity increased noteworthily by using the ethanolic extract by PSI, with values near 100 µg TE/g PLA.
PrC-210 Protects against Radiation-Induced Hematopoietic and Intestinal Injury in Mice and Reduces Oxidative Stress
Authors: Kumar Vidya P. , Biswas Shukla , Holmes-Hampton Gregory P. , Goesch Torsten , Fahl William , Ghosh Sanchita P.
Published: July 2023

The development of safe, orally available, and effective prophylactic countermeasures to protect our warfighters is an unmet need because there is no such FDA-approved countermeasure available for use. Th 1-Propanethiol, 3-(methylamino)-2-((methylamino)methyl) (PrC-210), a synthetic small molecule, is a member of a new family of aminothiols designed to reduce toxicity while scavenging reactive oxygen species (ROS). Our study investigated the protective role of a single oral administration of PrC-210 against radiation-induced hematopoietic and intestinal injury in mice. Pre-treatment with PrC-210 significantly improved the survival of mice exposed to a lethal dose of radiation. Our findings indicated that the radioprotective properties of PrC-210 are achieved by accelerating the recovery of the hematopoietic system, stimulating bone marrow progenitor cells, and ameliorating additional biomarkers of hematopoietic injury. PrC-210 pre-treatment reduced intestinal injury in mice exposed to a lethal dose of radiation by restoring jejunal crypts and villi, reducing translocation of bacteria to the spleen, maintaining citrulline levels, and reducing the sepsis marker serum amyloid A (SAA) in serum. Finally, PrC-210 pre-treatment led to a significant reduction (~10 fold) of Nos2 expression (inducible nitric oxide) in the spleen and decreased oxidative stress by enhancing the antioxidant defense system. These data support the further development of PrC-210 to receive approval from the FDA to protect warfighters and first responders from exposure to the harmful effects of ionizing radiation.
Exploitation of Quercetin’s Antioxidative Properties in Potential Alternative Therapeutic Options for Neurodegenerative Diseases
Authors: Rarinca Viorica , Nicoara Mircea Nicusor , Ureche Dorel , Ciobica Alin
Published: July 2023

Oxidative stress (OS) is a condition in which there is an excess of reactive oxygen species (ROS) in the body, which can lead to cell and tissue damage. This occurs when there is an overproduction of ROS or when the body’s antioxidant defense systems are overwhelmed. Quercetin (Que) is part of a group of compounds called flavonoids. It is found in high concentrations in vegetables, fruits, and other foods. Over the past decade, a growing number of studies have highlighted the therapeutic potential of flavonoids to modulate neuronal function and prevent age-related neurodegeneration. Therefore, Que has been shown to have antioxidant, anticancer, and anti-inflammatory properties, both in vitro and in vivo. Due to its antioxidant character, Que alleviates oxidative stress, thus improving cognitive function, reducing the risk of neurodegenerative diseases. On the other hand, Que can also help support the body’s natural antioxidant defense systems, thus being a potentially practical supplement for managing OS. This review focuses on experimental studies supporting the neuroprotective effects of Que in Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), and epilepsy.
Counteracting Roles of Lipidic Aldehydes and Phenolic Antioxidants on Soy Protein Oxidation Defined by a Chemometric Survey of Solvent and Mechanically Extracted Soybean Meals
Authors: Zhang Junwei , Urriola Pedro E. , Naeve Seth L. , Shurson Gerald C. , Chen Chi
Published: July 2023

Soybean meal (SBM) is a premier source of protein for feeding food-producing animals. However, its nutritional value can be compromised by protein oxidation. In this study, a total of 54 sources of solvent extracted SBM (SSBM) and eight sources of mechanically extracted SBM (MSBM), collected from different commercial producers and geographic locations in the United States during the years 2020 and 2021, were examined by chemometric analysis to determine the extent of protein oxidation and its correlation with soybean oil extraction methods and non-protein components. The results showed substantial differences between SSBM and MSBM in the proximate analysis composition, protein carbonyl content, lipidic aldehydes, and antioxidants, as well as subtle differences between 2020 SSBM and 2021 SSBM samples in protein oxidation and moisture content. Correlation analysis further showed positive correlations between protein carbonyl content and multiple lipid parameters, including the ether extract, p-anisidine value, individual aldehydes, and total aldehydes. Among the antioxidants in SBM, negative correlations with protein carbonyl content were observed for total phenolic content and isoflavone glycoside concentrations, but not for Trolox equivalent antioxidant capacity (TEAC), α-tocopherol, and γ-tocopherol. Overall, soybean oil extraction methods, together with other factors such as enzyme treatment and environmental conditions, can significantly affect the proximate analysis composition, the protein and lipid oxidation status, and the antioxidant profile of SBM. Lipidic aldehydes and phenolic antioxidants play counteracting roles in the oxidation of soy protein. The range of protein carbonyl content measured in this study could serve as a reference to evaluate the protein quality of SBM from various sources used in animal feed.
Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from Cannabis sativa
Authors: Perini Matteo , Gaggiotti Alessio , Pianezze Silvia , Ziller Luca , Larcher Roberto
Published: July 2023

Cannabidiol (CBD) is a non-psychoactive cannabinoid of Cannabis sativa that exhibits several beneficial pharmacological effects, including anti-inflammatory and antioxidant properties. The molecule can be obtained via extraction from the plant or through a biosynthetic route. The two products have both advantages and disadvantages, thus necessitating the development of methods capable of distinguishing between the two products. In this study, for the first time, the analysis of the stable isotope ratios of oxygen and hydrogen demonstrated high efficiency in the discrimination of CBD of a totally natural origin from that obtained through chemical synthesis. Considering a probability level of 95%, it was possible to identify threshold values for δ2H and δ18O of the totally natural CBD of −215‰ and +23.4‰, respectively. Higher values may indicate a non-entirely natural origin of CBD (i.e., a biosynthetic molecule).
Manoalide Induces Intrinsic Apoptosis by Oxidative Stress and Mitochondrial Dysfunction in Human Osteosarcoma Cells
Authors: Yao Zhi-Kang , Jean Yen-Hsuan , Lin Sung-Chun , Lai Yu-Cheng , Chen Nan-Fu , Tseng Chung-Chih , Chen Wu-Fu , Wen Zhi-Hong , Kuo Hsiao-Mei
Published: July 2023

Osteosarcoma (OS) is the most common primary malignant bone tumor that produces immature osteoid. Metastatic OS has a poor prognosis with a death rate of >70%. Manoalide is a natural sesterterpenoid isolated from marine sponges. It is a phospholipase A2 inhibitor with anti-inflammatory, analgesic, and anti-cancer properties. This study aimed to investigate the mechanism and effect of manoalide on OS cells. Our experiments showed that manoalide induced cytotoxicity in 143B and MG63 cells (human osteosarcoma). Treatment with manoalide at concentrations of 10, 20, and 40 µM for 24 and 48 h reduced MG63 cell viability to 45.13–4.40% (p < 0.01). Meanwhile, manoalide caused reactive oxygen species (ROS) overproduction and disrupted antioxidant proteins, activating the apoptotic proteins caspase-9/-3 and PARP (Poly (ADP-ribose) polymerase). Excessive levels of ROS in the mitochondria affected oxidative phosphorylation, ATP generation, and membrane potential (ΔΨm). Additionally, manoalide down-regulated mitochondrial fusion protein and up-regulated mitochondrial fission protein, resulting in mitochondrial fragmentation and impaired function. On the contrary, a pre-treatment with n-acetyl-l-cysteine ameliorated manoalide-induced apoptosis, ROS, and antioxidant proteins in OS cells. Overall, our findings show that manoalide induces oxidative stress, mitochondrial dysfunction, and apoptosis, causing the cell death of OS cells, showing potential as an innovative alternative treatment in human OS.
Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
Authors: Kostić Aleksandar Ž. , Milinčić Danijel D. , Špirović Trifunović Bojana , Nedić Nebojša , Gašić Uroš M. , Tešić Živoslav Lj. , Stanojević Sladjana P. , Pešić Mirjana B.
Published: July 2023

The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
Autophagy, Oxidative Stress, and Alcoholic Liver Disease: A Systematic Review and Potential Clinical Applications
Authors: Salete-Granado Daniel , Carbonell Cristina , Puertas-Miranda David , Vega-Rodríguez Víctor-José , García-Macia Marina , Herrero Ana Belén , Marcos Miguel
Published: July 2023

Ethanol consumption triggers oxidative stress by generating reactive oxygen species (ROS) through its metabolites. This process leads to steatosis and liver inflammation, which are critical for the development of alcoholic liver disease (ALD). Autophagy is a regulated dynamic process that sequesters damaged and excess cytoplasmic organelles for lysosomal degradation and may counteract the harmful effects of ROS-induced oxidative stress. These effects include hepatotoxicity, mitochondrial damage, steatosis, endoplasmic reticulum stress, inflammation, and iron overload. In liver diseases, particularly ALD, macroautophagy has been implicated as a protective mechanism in hepatocytes, although it does not appear to play the same role in stellate cells. Beyond the liver, autophagy may also mitigate the harmful effects of alcohol on other organs, thereby providing an additional layer of protection against ALD. This protective potential is further supported by studies showing that drugs that interact with autophagy, such as rapamycin, can prevent ALD development in animal models. This systematic review presents a comprehensive analysis of the literature, focusing on the role of autophagy in oxidative stress regulation, its involvement in organ–organ crosstalk relevant to ALD, and the potential of autophagy-targeting therapeutic strategies.
Globalized Knowledge-Based, Simulation-Driven Antenna Miniaturization Using Domain-Confined Surrogates and Dimensionality Reduction
Authors: Koziel Slawomir , Pietrenko-Dabrowska Anna , Golunski Lukasz
Published: July 2023

The design of contemporary antenna systems encounters multifold challenges, one of which is a limited size. Compact antennas are indispensable for new fields of application such as the Internet of Things or 5G/6G mobile communication. Still, miniaturization generally undermines electrical and field performance. When attempted using numerical optimization, it turns into a constrained problem with costly constraints requiring electromagnetic (EM) simulations. At the same time, due to the parameter redundancy of compact antennas, size reduction poses a multimodal task. In particular, the achievable miniaturization rate heavily depends on the starting point, while identifying a suitable starting point is a challenge on its own. These issues indicate that miniaturization should be addressed using global optimization methods. Unfortunately, the most popular nature-inspired algorithms cannot be applied for solving size reduction tasks because of their inferior computational efficacy and difficulties in handling constraints. This work proposes a novel methodology for the globalized size reduction of antenna structures. Our methodology is a multi-stage knowledge-based procedure, initialized with the detection of the approximate location of the feasible region boundary, followed by the construction of a dimensionality-reduced metamodel and global optimization thereof; the last stage is the miniaturization-oriented local refinement of geometry parameters. For cost reduction, the first stages of the procedure are realized with the use of a low-fidelity EM antenna model. Our approach is verified using four broadband microstrip antennas and benchmarked against multi-start local search as well as nature-inspired methods. Superior size reduction rates are demonstrated for all considered cases while maintaining reasonably low computational costs.
Three-Dimensional Joint Inversion of the Resistivity Method and Time-Domain-Induced Polarization Based on the Cross-Gradient Constraints
Authors: Zhu Depeng , Tan Handong , Peng Miao , Wang Tao
Published: July 2023

The resistivity method and time-domain-induced polarization (TDIP) are two branches of electric exploration that are used to solve problems in mineral exploration, hydrogeology and engineering geology. In recent years, integrating different physical parameters for joint inversion to improve the accuracy of inversion results has been extensively examined; however, three-dimensional joint inversion of the two methods above has not been realized. To further address this issue, in this research, we used the limited-memory BFGS (L-BFGS) method to develop a three-dimensional joint inversion algorithm of the resistivity method and TDIP based on the cross-gradient constraints. In the new algorithm, the resistivity method and TDIP inversion were iteratively updated alternately to ensure that the inversion results can simultaneously meet the two conditions of obtaining minimum data misfits and finding structural similarity. The three-dimensional synthetic dataset inversion results showed that the models obtained by joint inversion are more accurate in the recovery of both the boundaries and the values of the anomalies. Especially in the background of high noise, joint inversion has higher resolution for the target body. The joint inversion algorithm was also successfully applied to a groundwater detection practice in Beijing, China, in which the practicability of the algorithm was confirmed.
Finite Element Analysis (FEA) of the Stress and Strain Distribution in Cone-Morse Implant–Abutment Connection Implants Placed Equicrestally and Subcrestally
Authors: Di Pietro Natalia , Ceddia Mario , Romasco Tea , De Bortoli Junior Nilton , Mello Bruno Freitas , Tumedei Margherita , Specchiulli Alessandro , Piattelli Adriano , Trentadue Bartolomeo
Published: July 2023

Peri-implant bone resorption has been reported around some implants after loading, which could create problems for the peri-implant soft and hard tissues’ long-term stability. The reasons for this are still not known. However, relevant importance could be given to this due to the presence of a bacterial contamination at the micro-gap level between implant and abutment. In this regard, external and internal implant–abutment assemblies have been shown to be much more permeable to bacterial colonization than Cone-Morse or conical connections. The placement of a subcrestal implant could have aesthetic advantages, therefore allowing a better prosthetic emergence profile. In literature, controversial experimental and clinical results have been reported on bone resorption around implants placed equicrestally and subcrestally. Interestingly, Finite Element Analysis (FEA) studies revealed to be extremely useful for assessing the peri-implant bone strain and stress. Thus, this study conducted a FEA evaluation of implants with a Cone-Morse implant–abutment assembly inserted into a bone block model mimicking equicrestal (0 mm) and subcrestal placements (−1 and −2 mm). Results demonstrated that maximum stresses were observed in the cortical bone around equicrestally placed implants, with the lowest in the 2 mm subcrestally placed implant and intermediate stresses within the 1 mm subcrestally placed implant. The cortical bone resulted more stressed under lateral loads than axial loads. In conclusion, this FEA study suggested a subcrestal implant placement ranging between −1 and −2 mm to obtain an adequate peri-implant stress pattern.
The Contribution of Ski Poles to Aerodynamic Drag in Alpine Skiing
Authors: Supej Matej , Kalén Anton , Verdel Nina , Ogrin Jan , Holmberg Hans-Christer
Published: July 2023

The present study was designed to determine the contribution of the cross-sectional area of the ski poles (Sp) to the total aerodynamic drag during alpine skiing. At three different wind speeds in a wind tunnel, 10 skiers assumed typical alpine skiing postures (high, middle, and tuck), and their frontal aerodynamic drag was assessed with a force plate and their cross-sectional area, along with that of their ski poles, determined by interactive image segmentation. The data collected were utilized to examine intra-subject variation in Sp, the effects of Sp on the coefficient of aerodynamic drag (Cd), and the product of Cd and total cross-sectional area (Cd∙S. The major findings were as follows: (i) Sp ranged from 0.0067 (tuck position) to 0.0262 m2 (middle position), contributing 2.2–4.8% of the total cross-sectional area, respectively; (ii) Sp was dependent on wind speed in the high and middle positions; (iii) intra-subject variations ranged from 0.0018 m2 (27.6%) in the tuck position to 0.0072 m2 (30.5%) in the high position; (iv) Sp exerted a likely effect on Cd and Cd∙S. The extensive intra- and inter-skier variability in Sp can account for as much as ~5% of the total frontal cross-sectional area and future investigations on how elite skiers optimize their positioning of the poles in a manner that reduces aerodynamic drag are warranted.
The Low-Loss Spot Size Converter for Alignment with Cleaved Single Mode Fiber
Authors: Zhao Jinyang , Wang Zhutian , Ye Nan , Pang Fufei , Song Yingxiong
Published: July 2023

Integrating the graded index (GRIN) waveguide with the 2-D inversely-tapered waveguide, a new design of spot size converter (SSC) has been proposed to couple the light beam between the cleaved single-mode fiber (SMF) and the silicon (Si) wire waveguide on the silicon-on-insulator (SOI) platform. The device demonstrates a low coupling loss of 0.27 dB when it is coupled to a cleaved SMF. The polarization-dependent loss (PDL) is less than 0.25 dB, and the 1-dB alignment tolerance is about −2.0~2.0 µm for both vertical and horizontal directions. At the same time, the starting tip width of the tapered waveguide is kept at the level of the lithography limitation at the current commercial silicon photonics fab. This integrated SSC could be an improved design for reducing the coupling loss between the cleaved SMF and the Si waveguide.
Rapid Classification and Quantification of Coal by Using Laser-Induced Breakdown Spectroscopy and Machine Learning
Authors: Zheng Yanning , Lu Qingmei , Chen Anqi , Liu Yulin , Ren Xiaohan
Published: July 2023

Coal is expected to be an important energy resource for some developing countries in the coming decades; thus, the rapid classification and qualification of coal quality has an important impact on the improvement in industrial production and the reduction in pollution emissions. The traditional methods for the proximate analysis of coal are time consuming and labor intensive, whose results will lag in the combustion condition of coal-fired boilers. However, laser-induced breakdown spectroscopy (LIBS) assisted with machine learning can meet the requirements of rapid detection and multi-element analysis of coal quality. In this work, 100 coal samples from 11 origins were divided into training, test, and prediction sets, and some clustering models, classification models, and regression models were established for the performance analysis in different application scenarios. Among them, clustering models can cluster coal samples into several clusterings only by coal spectra; classification models can classify coal with labels into different categories; and the regression model can give quantitative prediction results for proximate analysis indicators. Cross-validation was used to evaluate the model performance, which helped to select the optimal parameters for each model. The results showed that K-means clustering could effectively divide coal samples into four clusters that were similar within the class but different between classes; naive Bayesian classification can distinguish coal samples into different origins according to the probability distribution function, and its prediction accuracy could reach 0.967; and partial least squares regression can reduce the influence of multivariate collinearity on prediction, whose root mean square error of prediction for ash, volatile matter, and fixed carbon are 1.012%, 0.878%, and 1.409%, respectively. In this work, the built model provided a reference for the selection of machine learning methods for LIBS when applied to classification and qualification.
Semi-Automated Mapping of German Study Data Concepts to an English Common Data Model
Authors: Chechulina Anna , Carus Jasmin , Breitfeld Philipp , Gundler Christopher , Hees Hanna , Twerenbold Raphael , Blankenberg Stefan , Ückert Frank , Nürnberg Sylvia
Published: July 2023

The standardization of data from medical studies and hospital information systems to a common data model such as the Observational Medical Outcomes Partnership (OMOP) model can help make large datasets available for analysis using artificial intelligence approaches. Commonly, automatic mapping without intervention from domain experts delivers poor results. Further challenges arise from the need for translation of non-English medical data. Here, we report the establishment of a mapping approach which automatically translates German data variable names into English and suggests OMOP concepts. The approach was set up using study data from the Hamburg City Health Study. It was evaluated against the current standard, refined, and tested on a separate dataset. Furthermore, different types of graphical user interfaces for the selection of suggested OMOP concepts were created and assessed. Compared to the current standard our approach performs slightly better. Its main advantage lies in the automatic processing of German phrases into English OMOP concept suggestions, operating without the need for human intervention. Challenges still lie in the adequate translation of nonstandard expressions, as well as in the resolution of abbreviations into long names.
Individual Differences in Signal Perception for Takeover Request in Autonomous Driving
Authors: Lee Okkeun , Kang Hyunmin
Published: July 2023

In the context of partial autonomy, where autonomous vehicles and humans share control of the vehicle, bringing out-of-the-loop drivers back into the loop is a significant challenge. While warning signal design guidelines are commonly used to provide alerts, few studies have examined each signal in depth with an emphasis on the autonomous environment. This study aims to fill this gap by investigating visual, auditory, and tactile stimuli and modifying their sub-attributes to explore variations related to age, gender, and other individual backgrounds. For this objective, the research examined the correlations between age, gender, and individual backgrounds with reaction times to TOR signals, investigating the effects of sub-attribute variations on participants’ responses and exploring the subjective evaluations of the signals. A driving simulator was utilized to create a realistic driving environment and measure participants’ reaction times in takeover request situations. Analysis of the data revealed correlations between age and reaction times for auditory and tactile signals, with interaction effects observed between age and sub-attribute intensity. Additionally, participants exhibited varying reaction time patterns in response to different sub-attribute intensities. By evaluating individual differences in perception based on modality characteristics, often overlooked in prior research, this study serves as a foundational contribution to future research in the field.