bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectiveness, cost-utility and physiological underpinnings of the FIBROWALK multicomponent therapy in online and outdoor format in individuals with fibromyalgia: Study protocol of a randomized, controlled trial (On&Out study)
Authors: Serrat Mayte , Ferrés Sònia , Auer William , Almirall Míriam , Lluch Enrique , D’Amico Francesco , Maes Michael , Lorente Sonia , Navarrete Jaime , Montero-Marín Jesús , Neblett Randy , Nijs Jo , Borràs Xavier , Luciano Juan V. , Feliu-Soler Albert
Published: November 2022

Introduction: The On&Out study is aimed at assessing the effectiveness, cost-utility and physiological underpinnings of the FIBROWALK multicomponent intervention conducted in two different settings: online (FIBRO-On) or outdoors (FIBRO-Out). Both interventions have proved to be efficacious in the short-term but there is no study assessing their comparative effectiveness nor their long-term effects. For the first time, this study will also evaluate the cost-utility (6-month time-horizon) and the effects on immune-inflammatory biomarkers and Brain-Derived Neurotrophic Factor (BDNF) levels of both interventions. The objectives of this 6-month, randomized, controlled trial (RCT) are 1) to examine the effectiveness and cost-utility of adding FIBRO-On or FIBRO-Out to Treatment-As-Usual (TAU) for individuals with fibromyalgia (FM); 2) to identify pre–post differences in blood biomarker levels in the three study arms and 3) to analyze the role of process variables as mediators of 6-month follow-up clinical outcomes.

Methods and analysis: Participants will be 225 individuals with FM recruited at Vall d’Hebron University Hospital (Barcelona, Spain), randomly allocated to one of the three study arms: TAU vs. TAU + FIBRO-On vs. TAU + FIBRO-Out. A comprehensive assessment to collect functional impairment, pain, fatigue, depressive and anxiety symptoms, perceived stress, central sensitization, physical function, sleep quality, perceived cognitive dysfunction, kinesiophobia, pain catastrophizing, psychological inflexibility in pain and pain knowledge will be conducted pre-intervention, at 6 weeks, post-intervention (12 weeks), and at 6-month follow-up. Changes in immune-inflammatory biomarkers [i.e., IL-6, CXCL8, IL-17A, IL-4, IL-10, and high-sensitivity C-reactive protein (hs-CRP)] and Brain-Derived Neurotrophic Factor will be evaluated in 40 participants in each treatment arm (total n = 120) at pre- and post-treatment. Quality of life and direct and indirect costs will be evaluated at baseline and at 6-month follow-up. Linear mixed-effects regression models using restricted maximum likelihood, mediational models and a full economic evaluation applying bootstrapping techniques, acceptability curves and sensitivity analyses will be computed.

Ethics and dissemination: This study has been approved by the Ethics Committee of the Vall d’Hebron Institute of Research. The results will be actively disseminated through peer-reviewed journals, conference presentations, social media and various community engagement activities. Trial registration number NCT05377567 (clinicaltrials.gov).
Impact of single biochar application on maize growth and water-fertilizer productivity under different irrigation regimes
Authors: Yang Wei , Feng Gary , Jia Yonglin , Yang Yixuan , Gao Xiaoyu , Gao Lihua , Qu Zhongyi
Published: November 2022

The improvement of soil water and nutrient availability through soil management practices are crucial in promoting crop growth and obtaining high water-fertilizer productivity under limited irrigation. In this study, a 2×4 fully randomized factorial design with two drip-irrigation regimes and four biochar rates was performed during maize crop growing seasons for a semiarid region of China in 2015 and 2016. Irrigation regimes was applied on the basis of the water lower limit of -15 kPa soil matric potential as W15 and -35 kPa as W35. Maize straw-derived biochar application rate of 0 (B0), 15 (B15), 30 (B30), and 45 (B45) t ha-1 was once applied to sandy loam soil in the first growing season. Our results showed that the W15 and W35 regimes generally increased soil nutrient availability and organic matter content under all biochar treatment rates for the entire growth period. In comparison, the B45-induced increase in available P and K was higher in the W15 regime than in the W35 regime during the second growing season. Furthermore, biochar treatment improved the comprehensive fertility index (CFI), leaf area index, and yield of maize. Within the same biochar treatment, the CFI value was higher in the W15 regime than in the W35 regime during the first growing season. However, the opposite was observed in the second growing season. The average irrigation water productivity (IWP) increased by 11.6%, 8.8%, and 7.8% in the W35 regime and by 15.2%, 12.9%, and 10.2% in the W15 regime for the B15, B30, and B45 treatments, respectively. Moreover, biochar treatment enhanced maize grain yield and partial fertilizer productivity (PFP) of synthetic N, P, and K fertilizers under both irrigation regimes. The highest PFP values were observed in the B15 treatment under W15. In general, a one-time application of biochar treatment at a rate of 15 t ha-1 in the first growing season is recommended in terms of increasing the availability of N, P, K, and organic matter in sandy loam and also improve water-fertilizer productivity under irrigation water lower limit of -15 kPa soil matric potential.
A graph-based approach for simultaneous semantic and instance segmentation of plant 3D point clouds
Authors: Mirande Katia , Godin Christophe , Tisserand Marie , Charlaix Julie , Besnard Fabrice , Hétroy-Wheeler Franck
Published: November 2022

Accurate simultaneous semantic and instance segmentation of a plant 3D point cloud is critical for automatic plant phenotyping. Classically, each organ of the plant is detected based on the local geometry of the point cloud, but the consistency of the global structure of the plant is rarely assessed. We propose a two-level, graph-based approach for the automatic, fast and accurate segmentation of a plant into each of its organs with structural guarantees. We compute local geometric and spectral features on a neighbourhood graph of the points to distinguish between linear organs (main stem, branches, petioles) and two-dimensional ones (leaf blades) and even 3-dimensional ones (apices). Then a quotient graph connecting each detected macroscopic organ to its neighbors is used both to refine the labelling of the organs and to check the overall consistency of the segmentation. A refinement loop allows to correct segmentation defects. The method is assessed on both synthetic and real 3D point-cloud data sets of Chenopodium album (wild spinach) and Solanum lycopersicum (tomato plant).
A story of resilience: Arctic diatom Chaetoceros gelidus exhibited high physiological plasticity to changing CO2 and light levels
Authors: Biswas Haimanti
Published: November 2022

Arctic phytoplankton are experiencing multifaceted stresses due to climate warming, ocean acidification, retreating sea ice, and associated changes in light availability, and that may have large ecological consequences. Multiple stressor studies on Arctic phytoplankton, particularly on the bloom-forming species, may help understand their fitness in response to future climate change, however, such studies are scarce. In the present study, a laboratory experiment was conducted on the bloom-forming Arctic diatom Chaetoceros gelidus (earlier C. socialis) under variable CO2 (240 and 900 µatm) and light (50 and 100 µmol photons m-2 s-1) levels. The growth response was documented using the pre-acclimatized culture at 2°C in a closed batch system over 12 days until the dissolved inorganic nitrogen was depleted. Particulate organic carbon and nitrogen (POC and PON), pigments, cell density, and the maximum quantum yield of photosystem II (Fv/Fm) were measured on day 4 (D4), 6 (D6), 10 (D10), and 12 (D12). The overall growth response suggested that C. gelidus maintained a steady-state carboxylation rate with subsequent conversion to macromolecules as reflected in the per-cell POC contents under variable CO2 and light levels. A substantial amount of POC buildup at the low CO2 level (comparable to the high CO2 treatment) indicated the possibility of existing carbon dioxide concentration mechanisms (CCMs) that needs further investigation. Pigment signatures revealed a high level of adaptability to variable irradiance in this species without any major CO2 effect. PON contents per cell increased initially but decreased irrespective of CO2 levels when nitrogen was limited (D6 onward) possibly to recycle intracellular nitrogen resources resulting in enhanced C: N ratios. On D12 the decreased dissolved organic nitrogen levels could be attributed to consumption under nitrogen starvation. Such physiological plasticity could make C. gelidus “ecologically resilient” in the future Arctic.
Molecular mechanism of Cu metal and drought stress resistance triggered by Porostereum spadiceum AGH786 in Solanum lycopersicum L.
Authors: Naz Falak , Hamayun Muhammad , Rauf Mamoona , Arif Muhammad , Afzal Khan Sumera , Ud-Din Jalal , Gul Humaira , Hussain Anwar , Iqbal Amjad , Kim Ho-Youn , Lee In-Jung
Published: November 2022

Rapid industrialization and global warming have threatened the plants with multiple abiotic stresses, such as heavy metals and drought stress. For crop cultivation, the conventional approach of cleaning the soils by excavation is very costly and not feasible for large scale. Establishing toxin-free and drought-resistant crops is a major challenge in the environment under natural and anthropogenic pressure. In the past decades, copper contamination of agricultural land has become an emerging concern. For dry land reclamation, several new strategies, including bioremediation (phytoremediation and microbial remediation), have been used. Owing to the potential of Cu hyperaccumulators, the current project aims to enhance the drought tolerance and the phytoremediation potential of Solanum lycopersicum L. with the inoculation of copper and 12% polyethylene glycol (PEG)–induced drought stress–tolerant endophytic fungus Porostereum spadiceum AGH786 under the combined stress of copper heavy metal and PEG-induced drought stress. When S. lycopersicum L. was watered with individual stress of copper (Cu) concentration (400 ppm) in the form of copper sulfate (CuSO4.5H2O), 12% PEG–induced drought stress and the combined stress of both negatively affected the growth attributes, hormonal, metabolic, and antioxidant potential, compared with control. However, the multistress-resistant AGH786 endophytic fungus ameliorated the multistress tolerance response in S. lycopersicum L. by positively affecting the growth attributes, hormonal, metabolic, and antioxidant potential, and by restricting the root-to-shoot translocation of Cu and inducing its sequestration in the root tissues of affected plants. AGH786-associated plants exhibited a reduction in the severity of copper (Cu) and drought stress, with higher levels of SlCOPT (Cu transporters) and SlMT (metallothionine) gene expressions in root and shoot tissues, indicating that AGH786 contributed to resistance to copper metal toxicity and drought stress in the host S. lycopersicum L.
The end-use quality of wheat can be enhanced by optimal water management without incurring yield loss
Authors: Sheng Kun , Xu Lina , Wang Mingxia , Lei Heng , Duan Aiwang
Published: November 2022

In China, water-saving irrigation is playing important roles in ensuring food security, and improving wheat quality. A barrel experiment was conducted with three winter wheat (Triticum aestivum L.) genotypes and two irrigation pattens to examine the effects of regulated deficit irrigation (RDI) on wheat grain yield, water-use efficiency (WUE), and grain quality. In order to accurately control the soil water content, wheat was planted in the iron barrels set under a rainproof shelter, and the soil water content in the iron barrel was controlled by gravity method. The mechanisms whereby water management influences the end-use functional properties of wheat grain were also investigated. The results revealed that RDI improved the end-use functional properties of wheat and WUE, without significant yield loss (less than 3%). Moderate water deficit (60% to 65% field capacity) before jointing and during the late grain-filling stage combined with a slight water deficit (65% to 70% field capacity) from jointing to booting increased grain quality and WUE. The observed non-significant reduction in wheat yield associated with RDI may be attributed to higher rate of photosynthesis during the early stage of grain development and higher rate of transfer of carbohydrates from vegetative organs to grains during the later stage. By triggering an earlier rapid transfer of nitrogen deposited in vegetative organs, RDI enhances grain nitrogen content, which in turn could enhance dough elasticity, given the positive correlation between grain nitrogen content and dough midline peak value. Our results also indicate that the effects of RDI on grain quality are genotype dependent. Therefore, the grain end-use quality of some specific wheat genotypes may be enhanced without incurring yield loss by an optimal water management.
A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening
Authors: Xiao Yunyi , Li Ying , Ouyang Lejun , Yin Aiguo , Xu Bo , Zhang Ling , Chen Jianye , Liu Jinfeng
Published: November 2022

Fruit postharvest ripening is a crucial course for many fruits with significant conversion of biosubstance, which forms an intricate regulatory network. Ethylene facilitates the ripening process in banana with a remarkable change of fruit starch, but the mechanism adjusting the expression of starch degradation-related enzyme genes is incompletely discovered. Here, we describe a banana APETALA2 transcription factor (MaAP2a) identified as a transcriptional repressor with its powerful transcriptional inhibitory activity. The transcriptional level of MaAP2a gradually decreased with the transition of banana fruit ripening, suggesting a passive role of MaAP2a in banana fruit ripening. Moreover, MaAP2a is a classic nucleoprotein and encompasses transcriptional repressor domain (EAR, LxLxLx). More specifically, protein–DNA interaction assays found that MaAP2a repressed the expression of 15 starch degradation-related genes comprising MaGWD1, MaPWD1, MaSEX4, MaLSF1, MaBAM1-MaBAM3, MaAMY2B/2C/3A/3C, MaMEX1/2, and MapGlcT2-1/2-2 via binding to the GCC-box or AT-rich motif of their promoters. Overall, these results reveal an original MaAP2a-mediated negative regulatory network involved in banana postharvest starch breakdown, which advances our cognition on banana fruit ripening and offers additional reference values for banana varietal improvement.
Mechanisms involved in drought stress tolerance triggered by rhizobia strains in wheat
Authors: Barquero Marcia , Poveda Jorge , Laureano-Marín Ana M. , Ortiz-Liébana Noemí , Brañas Javier , González-Andrés Fernando
Published: November 2022

Rhizobiumspp. is a well-known microbial plant biostimulant in non-legume crops, but little is known about the mechanisms by which rhizobia enhance crop productivity under drought stress. This work analyzed the mechanisms involved in drought stress alleviation exerted byRhizobium leguminosarumstrains in wheat plants under water shortage conditions. Two (LBM1210 and LET4910) of the fourR.leguminosarumstrains significantly improved the growth parameters (fresh and dry aerial weight, FW and DW, respectively), chlorophyll content, and relative water content (RWC) compared to a non-inoculated control under water stress, providing values similar to or even higher for FW (+4%) and RWC (+2.3%) than the non-inoculated and non-stressed control. Some other biochemical parameters and gene expression explain the observed drought stress alleviation, namely the reduction of MDA, H2O2(stronger when inoculating with LET4910), and ABA content (stronger when inoculating with LBM1210). In agreement with these results, inoculation with LET4910 downregulatedDREB2andCAT1genes in plants under water deficiency and upregulated theCYP707A1gene, while inoculation with LBM1210 strongly upregulated theCYP707A1gene, which encodes an ABA catabolic enzyme. Conversely, from our results, ethylene metabolism did not seem to be involved in the alleviation of drought stress exerted by the two strains, as the expression of theCTR1gene was very similar in all treatments and controls. The obtained results regarding the effect of the analyzed strains in alleviating drought stress are very relevant in the present situation of climate change, which negatively influences agricultural production.
LjNRT2.3 plays a hierarchical role in the control of high affinity transport system for root nitrate acquisition in Lotus japonicus
Authors: Rogato Alessandra , Valkov Vladimir Totev , Chiurazzi Maurizio
Published: November 2022

Nitrate is a key mineral nutrient required for plant growth and development. Plants have evolved sophisticated mechanisms to respond to changes of nutritional availability in the surrounding environment and the optimization of root nitrate acquisition under nitrogen starvation is crucial to cope with unfavoured condition of growth. In this study we present a general description of the regulatory transcriptional and spatial profile of expression of theLotus japonicusnitrate transporterNRT2family. Furthermore, we report a phenotypic characterization of two independentLjnrt2.3knock out mutants indicating the involvement of theLjNRT2.3gene in the root nitrate acquisition and lateral root elongation pathways occurring in response to N starvation conditions. We also report an epistatic relationship betweenLjNRT2.3andLjNRT2.1suggesting a combined mode of action of these two genes in order to optimize theLotusresponse to a prolonged N starvation.
A plant growth-promoting bacteria Priestia megaterium JR48 induces plant resistance to the crucifer black rot via a salicylic acid-dependent signaling pathway
Authors: Li Qi , Hou Zhaoqi , Zhou Dongqin , Jia Mingyun , Lu Shipeng , Yu Jinping
Published: November 2022

Xanthomonas campestris pv. campestris (Xcc)-induced black rot is one of the most serious diseases in cruciferous plants. Using beneficial microbes to control this disease is promising. In our preliminary work, we isolated a bacterial strain (JR48) from a vegetable field. Here, we confirmed the plant-growth-promoting (PGP) effects of JR48 in planta, and identified JR48 as a Priestia megaterium strain. We found that JR48 was able to induce plant resistance to Xcc and prime plant defense responses including hydrogen peroxide (H2O2) accumulation and callose deposition with elevated expression of defense-related genes. Further, JR48 promoted lignin biosynthesis and raised accumulation of frees salicylic acid (SA) as well as expression of pathogenesis-related (PR) genes. Finally, we confirmed that JR48-induced plant resistance and defense responses requires SA signaling pathway. Together, our results revealed that JR48 promotes plant growth and induces plant resistance to the crucifer black rot probably through reinforcing SA accumulation and response, highlighting its potential as a novel biocontrol agent in the future.
Exploring and exploiting cuticle biosynthesis for abiotic and biotic stress tolerance in wheat and barley
Authors: Wang Xiaoyu , Chang Cheng
Published: November 2022

Wheat and barley are widely distributed cereal crops whose yields are adversely affected by environmental stresses such as drought, salinity, extreme temperatures, and attacks of pathogens and pests. As the interphase between aerial plant organs and their environments, hydrophobic cuticle largely consists of a cutin matrix impregnated and sealed with cuticular waxes. Increasing evidence supports that the cuticle plays a key role in plant adaptation to abiotic and biotic stresses, which could be harnessed for wheat and barley improvement. In this review, we highlighted recent advances in cuticle biosynthesis and its multifaceted roles in abiotic and biotic stress tolerance of wheat and barley. Current strategies, challenges, and future perspectives on manipulating cuticle biosynthesis for abiotic and biotic stress tolerance in wheat and barley are discussed.
Measurement of morphological changes of pear leaves in airflow based on high-speed photography
Authors: Zhang Chao , Zhou Hongping , Xu Linyun , Ru Yu , Ju Hao , Chen Qing
Published: November 2022

The morphological changes of leaves under the airflow have a significant effect on the deposition of pesticide droplets on the leaves, but the wind-induced vibration of the leaves is complicated to measure. In this study, an aerodynamic test of the pear leaf was conducted in the wind tunnel, and binocular high-speed photography was used to record the deformation and vibration of the leaves under various airflow velocities. Experiments showed that air velocity (v) had a significant effect on the morphological response of the leaf. As v increased, the leaf was in three states, including static deformation, low-frequency vibration, and reconfiguration of airfoil steady state. The mutation from one state to another occurred at the critical velocity of vcr1and vcr2. By tracking the leaf marker point, various morphological parameters were calculated, including the bending angle of the petiole, the wind deflection angle, and the twist angle of leaves under different air velocities. When vcr1 ≤v ≤vcr2, the parameters changed periodically. When v< vcr1, the petiole and the leaf bent statically, and the bending angle of the petiole and the wind deflection angle of the leaf gradually increased. When v >vcr2, the morphology of the leaf and the petiole was stable. Besides, this study tracked and measured the wind deflection area of leaf, which was consistent with the theoretical calculation results. The measurement of the leaf morphological parameters can reflect the morphological changes of leaves under airflow, thus providing a basis for the decision-making of air-assisted spray airflow.
Stronger wind, smaller tree: Testing tree growth plasticity through a modeling approach
Authors: Wang Haoyu , Hua Jing , Kang Mengzhen , Wang Xiujuan , Fan Xing-Rong , Fourcaud Thierry , de Reffye Philippe
Published: November 2022

Plants exhibit plasticity in response to various external conditions, characterized by changes in physiological and morphological features. Although being non-negligible, compared to the other environmental factors, the effect of wind on plant growth is less extensively studied, either experimentally or computationally. This study aims to propose a modeling approach that can simulate the impact of wind on plant growth, which brings a biomechanical feedback to growth and biomass distribution into a functional–structural plant model (FSPM). Tree reaction to the wind is simulated based on the hypothesis that plants tend to fit in the environment best. This is interpreted as an optimization problem of finding the best growth-regulation sink parameter giving the maximal plant fitness (usually seed weight, but expressed as plant biomass and size). To test this hypothesis in silico, a functional–structural plant model, which simulates both the primary and secondary growth of stems, is coupled with a biomechanical model which computes forces, moments of forces, and breakage location in stems caused by both wind and self-weight increment during plant growth. The Non-dominated Sorting Genetic Algorithm II (NSGA-II) is adopted to maximize the multi-objective function (stem biomass and tree height) by determining the key parameter value controlling the biomass allocation to the secondary growth. The digital trees show considerable phenotypic plasticity under different wind speeds, whose behavior, as an emergent property, is in accordance with experimental results from works of literature: the height and leaf area of individual trees decreased with wind speed, and the diameter at the breast height (DBH) increased at low-speed wind but declined at higher-speed wind. Stronger wind results in a smaller tree. Such response of trees to the wind is realistically simulated, giving a deeper understanding of tree behavior. The result shows that the challenging task of modeling plant plasticity may be solved by optimizing the plant fitness function. Adding a biomechanical model enriches FSPMs and opens a wider application of plant models.
Effects of HIV-related worries on fertility motivation moderated by living children among couples living with HIV: A dyadic analysis
Authors: Guo Yingwu , Du Yingrong , Liu Jun , Bai Jingsong , Gao Jianpeng , Wu Lei , Yang Yongrui , Wang Weibo , Chen Jie , Xu Zhaoyuan , Yan Junfang , Lu Nihong , Li Chongxi , Chongsuvivatwong Virasakdi
Published: November 2022

Introduction

HIV-related worries are a major barrier to achieving fertility goals for couples living with HIV (CLWH). We examined the moderating role of living children in the association between HIV-related worries and fertility motivation in CLWH including happiness, well-being, identity, and continuity.

Methods

The data of 322 reproductive-aged CLWH were collected for this cross-sectional study from a referral antiretroviral therapy clinic in Kunming, China between October and December 2020. Intra- and interpersonal mechanisms of association between HIV-related worries and fertility motivation moderated by the number of living children in husband-wife dyads were analyzed by the actor-partner interdependence moderation model.

Results

The high-level HIV-related worries of the wives and husbands were associated with the spouses’ fertility motivation. Having at least one child helped to ameliorate the negative association between one’s own HIV-related worries and fertility motivation. However, there was no evidence of such moderation in the spouse.

Conclusion

Whether the CLWH has at least one living child should be taken into account in counseling. Childless families should be counseled on HIV-related worries as those worries have a greater negative effect on fertility motivation than couples who have a child.
Early-childhood temperament moderates the prospective associations of coping with adolescent internalizing and externalizing symptoms
Authors: Smith Michele R. , Parrish Krystal H. , Shimomaeda Lisa , Zalewski Maureen , Rosen Maya L. , Rodman Alexandra , Kasparek Steven , Mayes Makeda , Meltzoff Andrew N. , McLaughlin Katie A. , Lengua Liliana J.
Published: November 2022

While appraisal and coping are known to impact adolescent psychopathology, more vulnerable or resilient responses to stress may depend on individual temperament. This study examined early life temperament as a moderator of the prospective relations of pre-adolescent appraisal and coping with adolescent psychopathology. The sample included 226 (62% female, 14–15 years) adolescents with assessments starting at 3 years of age. Adolescents were predominately White (12% Black 9% Asian, 11% Latinx, 4% Multiracial, and 65% White). Observed early-childhood temperament (fear, frustration, executive control, and delay ability) were tested as moderators of pre-adolescent coping (active and avoidant) and appraisal (threat, positive) on internalizing and externalizing symptoms during the pandemic. Interaction effects were tested using regression in R. Sex and family context of stress were covariates. Early-childhood temperament was correlated with pre-adolescent symptoms, however, pre-adolescent appraisal and coping but not temperament predicted adolescent psychopathology. Frustration moderated the relations of active and avoidant coping and positive appraisal to symptoms such that coping and appraisal related to lower symptoms only for those low in frustration. Executive control moderated the associations of avoidant coping with symptoms such that avoidance reduced the likelihood of symptoms for youth low in executive control. Findings underscore the role of emotionality and self-regulation in youth adjustment, with the impact of coping differing with temperament. These findings suggest that equipping youth with a flexible assortment of coping skills may serve to reduce negative mental health outcomes.
A motivational approach to perfectionism and striving for excellence: Development of a new continuum-based scale for post-secondary students
Authors: Lasalle Marie , Hess Ursula
Published: November 2022

Perfectionism has been the object of many disputes. One such debate pertains to the nature of perfectionistic strivings. Whereas perfectionistic concerns (PC) have been shown to correlate with negative outcomes, perfectionistic strivings (PS) have been associated with mixed outcomes. This view of perfectionism assumes a motivational perspective; however, commonly used questionnaires assess motivation only implicitly. To create a more explicit measure of motivation as regard perfectionism, we aimed to assess perfectionism in post-secondary education based on Deci and Ryan’s self-determination continuum. We posit that introjected motivation represents the variance common to both dimensions of perfectionism. External motivation is considered to be specific to PC and identified motivation to PS. Amotivation represents a lack of meaningful striving. Intrinsic motivation, lacking perfectionism’s pressure, is conceptualized to be a self-determined form of striving for excellence. We further posited that this continuum is implicitly underlain by a continuum of self-definition as defined by Blatt. The resulting questionnaire showed an adequate structure with ESEM, followed a simplex structure, and had adequate reliabilities (Study 1a/Study 2). It also showed adequate convergent validity (Study 1b/Study 2). Finally, the questionnaire proved to be invariant over a 6-week period (Study 2). Results suggest that the degree of active goal pursuit, in addition to standards setting, could be a distinguishing characteristic between dimensions of perfectionism as well as striving for excellence.
Nature play in early childhood education: A systematic review and meta ethnography of qualitative research
Authors: Prins Jannette , van der Wilt Femke , van der Veen Chiel , Hovinga Dieuwke
Published: November 2022

Play in nature-based environments in childhood education has positive benefits for child development. Although previous reviews showed the benefits of play in nature-based environments for child development they did not attempt to understand how and why nature-based environments contribute to play quality. This review aims to explore the value of play in nature-based environments compared to non-nature-based environments for developmental outcomes of young children (2–8 year). We searched for studies that investigated the relation between play and nature-based environments on the databases PsycINFO, ERIC, and Web of Science. Inclusion/exclusion criteria were: (1) the study focused on play in/on a nature based environment, (2) the study included participants between the age of 2–8 years, (3) it was an empirical study, (4) the study was conducted in the context of early childhood education (ECE), and (5) the study included participants without special needs or disabilities. Using these criteria we selected 28 qualitative studies with an overall sample size of N = 998 children aged 2–8 years. The studies were synthesized using an adaptation of Noblit and Hare’s meta-ethnographic approach. Three overarching themes were found: (1) the aspects of play quality that are related to nature-based environments, (2) the aspects of nature-based environments that support play, and (3) the aspects of teacher-child interactions that contribute to nature play quality. The meta themes resonate with play theories and theories of the restorative value of nature. We draw on the qualitative data to refine and extend these theories, and to come up with a definition of the concept “nature play.” This systematic review also sets a base for future research on play interventions in nature-based environments. We argue that (1) research will benefit from thoroughly conceptualizing the role of play in the development of young children, (2) using the affordances theory research will benefit from moving beyond the individual play actions as a unit of analysis, and (3) from an educational perspective it is important to shift the focus of nature play to its benefits for children’s cognitive development.
Case report: Delusional infestation in dementia with Lewy bodies
Authors: Taomoto Daiki , Kanemoto Hideki , Satake Yuto , Yoshiyama Kenji , Iwase Masao , Hashimoto Mamoru , Ikeda Manabu
Published: November 2022

Background

Delusional infestation is characterized by delusions of being infested with parasites, vermin, or small insects and is frequently accompanied by tactile and visual hallucinations. Herein, we report two cases of dementia with Lewy bodies (DLB) with delusional infestation.

Case presentation

Case 1 was an 83-year-old man. At the age of 75, he began to show symptoms of rapid eye movement sleep behavior disorder. At the age of 83, he began to complain of visual hallucinations of people and delusional infestation with tactile and visual hallucinations of insects, resulting in the use of insecticides for non-existent insects. He also complained of mild amnesia and was admitted to our psychiatric ward for evaluation and treatment. After admission, the delusional infestation disappeared without any new medication. Based on our examinations, he was diagnosed with probable DLB with delusional infestation. He was treated with 5 mg/day of donepezil hydrochloride; his visual and tactile hallucinations disappeared, and the delusional infestation had not recurred at the 1-year follow-up. Case 2 was a 69-year-old woman. At the age of 60, she underwent clipping for subarachnoid hemorrhage (SAH). At the age of 65, she began to have visual hallucinations of people. At the age of 67, she began to complain of visual illusions in which she mistook lint for insects. At the age of 69, she developed delusional infestation and mild amnesia. She took various actions to get rid of these non-existent insects, including insecticide use, consulting an exterminator, and visiting several dermatologists. She eventually burnt her leg in an attempt to kill the non-existent insects. Based on our examinations, she was diagnosed with prodromal DLB in addition to SAH sequelae. We determined that her delusional infestation was caused by DLB rather than SAH sequelae based on the course of her symptoms. She was treated with a combination of 3 mg/day of donepezil hydrochloride and 12.5 mg/day of quetiapine. Thereafter, the delusional infestation partially improved, and she took no further action against non-existent insects.

Conclusion

Delusional infestation may be caused by DLB. Acetylcholinesterase inhibitors (AChEI) may be effective for delusional infestation in DLB, although antipsychotics may also be needed in severe cases.
Awareness of age-related changes in Norwegian individuals 50+. Short form questionnaire validation
Authors: Testad Ingelin , Ushakova Anastasia , Aakre Jon Arild , Sabatini Serena , Gjestsen Martha Therese
Published: November 2022

Background

A questionnaire assessing awareness of positive and negative age-related changes (AARC gains and losses) was developed in the US and Germany, and validated for the UK and Brazilian populations. In this study, we validated the short-form measure (AARC-10 SF) in the Norwegian population aged 50 and over. In addition, the relationship between cognitive variables and AARC was examined.

Methods

Cross-sectional analyses of data from 1,510 participants in the ongoing online PROTECT Norge study were used to explore and confirm the two-factor structure of AARC gains and AARC losses; reliability; measurement invariance across different population groups defined by sex, education level, employment, and in middle age, early old age, and advanced old age. We explored the relationship between AARC and demographic variables (defined in the same way as the population groups).

Results

We confirmed the two-factor structure (gains and losses) of the Norwegian translation of the AARC-10 SF. We did not find mutual correlations between related items in gains and losses, except for the physical health item from the gains dimension, which was positively correlated with all items of the losses dimension. Age, sex, marital status, employment, and university education predicted AARC gains and losses.

Conclusion

The Norwegian translation of the AARC-10 SF captures individuals’ positive and negative self-perceptions of age-related changes in their mental, physical, and cognitive health.
A genetic association study reveals the relationship between the oral microbiome and anxiety and depression symptoms
Authors: Li Chun'e , Chen Yujing , Wen Yan , Jia Yumeng , Cheng Shiqiang , Liu Li , Zhang Huijie , Pan Chuyu , Zhang Jingxi , Zhang Zhen , Yang Xuena , Meng Peilin , Yao Yao , Zhang Feng
Published: November 2022

Background

Growing evidence supports that alterations in the gut microbiota play an essential role in the etiology of anxiety, depression, and other psychiatric disorders. However, the potential effect of oral microbiota on mental health has received little attention.

Methods

Using the latest genome-wide association study (GWAS) summary data of the oral microbiome, polygenic risk scores (PRSs) of 285 salivary microbiomes and 309 tongue dorsum microbiomes were conducted. Logistic and linear regression models were applied to evaluate the relationship between salivary-tongue dorsum microbiome interactions with anxiety and depression. Two-sample Mendelian randomization (MR) was utilized to compute the causal effects between the oral microbiome, anxiety, and depression.

Results

We observed significant salivary-tongue dorsum microbiome interactions related to anxiety and depression traits. Significantly, one common interaction was observed to be associated with both anxiety score and depression score, Centipeda periodontii SGB 224 × Granulicatella uSGB 3289 (P depressionscore = 1.41 × 10−8, P anxietyscore = 5.10 × 10−8). Furthermore, we detected causal effects between the oral microbiome and anxiety and depression. Importantly, we identified one salivary microbiome associated with both anxiety and depression in both the UKB database and the Finngen public database, Eggerthia (P IVW − majordepression − UKB = 2.99 × 10−6, P IVW − Self − reportedanxiety/panicattacks − UKB = 3.06 × 10−59, P IVW − depression − Finngen = 3.16 × 10,-16 P IVW − anxiety − Finngen = 1.14 × 10−115).

Conclusion

This study systematically explored the relationship between the oral microbiome and anxiety and depression, which could help improve our understanding of disease pathogenesis and propose new diagnostic targets and early intervention strategies.
MDMA for the treatment of misophonia, a proposal
Authors: Webb Jadon , Keane Shannon
Published: November 2022

Misophonia is a disorder characterized by negative physical and emotional reactions to certain trigger sounds, such as chewing food. Up to 50% of population samples endorse some symptoms of misophonia, with about 20% having symptoms that impair normal life functioning. Most misophonia patients exhibit intense negative emotions and autonomic arousal (the fight-flight-freeze response) in response to a trigger, similarly to how someone with post-traumatic stress disorder (PTSD) might respond to a trauma trigger. Curiously, misophonia trigger sounds are often most distressing when coming from a specific person, suggesting the disorder may be responsive to interpersonal relationship factors. Treatment of misophonia is currently limited to the use of hearing modifications (e.g., earplugs or headphones) and psychotherapy, but many patients continue to suffer despite these best efforts. Phase 3 clinical trials suggest that MDMA is efficacious at treating the symptoms of autonomic arousal, negative emotions, and interpersonal suffering found in PTSD. As such, we propose that MDMA may represent an ideal treatment for some suffering from severe misophonia. In this perspective article, we review the symptoms of misophonia, and outline how MDMA may be uniquely suited for treating it, perhaps using a protocol analogous to the MAPS Phase 3 studies for PTSD.
The major risk factor for depression in the Chinese middle-aged and elderly population: A cross-sectional study
Authors: Ni Xiaolin , Su Huabin , Lv Yuan , Li Rongqiao , Chen Chen , Zhang Di , Chen Qing , Zhang Shenqi , Yang Ze , Sun Liang , Zhou Qi , Zhu Xiaoquan , Gao Danni , Fang Sihang , Hu Caiyou , Pang Guofang , Yuan Huiping
Published: November 2022

Background

The number of patients suffering from depression is continuously increasing in China. Demographic characteristics, physical health levels, and individual lifestyles/healthy behaviors are associated with the severity of depression. However, the major risk factor for depression remains unclear.

Materials and methods

In this investigation, 16,512 patients were screened using the CHARLS (China Health and Retirement Longitudinal Study) database after being determined to be eligible based on the inclusion criteria. Depressive symptoms were evaluated through the CESD-10 (10-item Center for Epidemiological Studies Depression Scale). Consequently, various models were developed based on potential predictive factors, employing stepwise LR (Logistic Regression)/RF (Random Forests) models to examine the influence and weighting of candidate factors that affect depression.

Results

Gender, residential address location, changes in health status following last interview, physical disabilities, chronic pain, childhood health status, ADL (activity of daily living), and social activity were all revealed to be independent risk factors for depression (p < 0.05) in this study. Depression has a synergic effect (across chronic pain and age groups). In comparison to other factors, RF results showed that chronic pain had a stronger impact on depression.

Conclusion

This preliminary study reveals that chronic pain is a major risk factor for depression.
Legal responses to child endangerment on farms: Research methods
Authors: Benny Christopher P. , Beyer Dorianne , Krolczyk Madeline , Lee Barbara C.
Published: November 2022

In the US agriculture (including ranching) is among the most dangerous industries and it is the only one where children of any age are permitted in the worksite. Whether working or not, children are at risk of serious injury or death when present among the many hazards associated with agricultural work. In most cases the proximate cause of a traumatic incident involving a child (<18 years) is an adult's choice to allow the child's presence in a high-risk situation. Yet, little is known about the legal repercussions for a responsible adult when such events occur. With an overarching goal to enhance the culture of safety for children in agricultural settings, this project includes three phases: (1) identification and collection of public records and news reports regarding legal action following a childhood agricultural injury or fatality; (2) analysis of the proposed or imposed legal responses following these agricultural injuries and fatalities; and (3) development of recommendations for public agencies responding to events that lead to a criminal complaint or the imposition of non-criminal child welfare or other civil measures. This paper describes the project's mixed methods study design that yielded extensive details on 12 legal cases as well as perspectives from key informants on the strengths and limitations of legal responses to child endangerment on farms. Integration and analyses of data from quantitative and qualitative sources will be used to generate recommendations, including guidelines and protocols, for key stakeholder groups.
Genome-wide SNPs reveal novel patterns of spatial genetic structure in Aedes albopictus (Diptera Culicidae) population in China
Authors: Wei Yong , He Song , Wang Jiatian , Fan Peiyang , He Yulan , Hu Ke , Chen Yulan , Zhou Guofa , Zhong Daibin , Zheng Xueli
Published: November 2022

Introduction

Since the second half of the 20th century, Aedes albopictus, a vector for more than 20 arboviruses, has spread worldwide. Aedes albopictus is the main vector of infectious diseases transmitted by Aedes mosquitoes in China, and it has caused concerns regarding public health. A comprehensive understanding of the spatial genetic structure of this vector species at a genomic level is essential for effective vector control and the prevention of vector-borne diseases.

Methods

During 2016–2018, adult female Ae. albopictus mosquitoes were collected from eight different geographical locations across China. Restriction site-associated DNA sequencing (RAD-seq) was used for high-throughput identification of single nucleotide polymorphisms (SNPs) and genotyping of the Ae. albopictus population. The spatial genetic structure was analyzed and compared to those exhibited by mitochondrial cytochrome c oxidase subunit 1 (cox1) and microsatellites in the Ae. albopictus population.

Results

A total of 9,103 genome-wide SNP loci in 101 specimens and 32 haplotypes of cox1 in 231 specimens were identified in the samples from eight locations in China. Principal component analysis revealed that samples from Lingshui and Zhanjiang were more genetically different than those from the other locations. The SNPs provided a better resolution and stronger signals for novel spatial population genetic structures than those from the cox1 data and a set of previously genotyped microsatellites. The fixation indexes from the SNP dataset showed shallow but significant genetic differentiation in the population. The Mantel test indicated a positive correlation between genetic distance and geographical distance. However, the asymmetric gene flow was detected among the populations, and it was higher from south to north and west to east than in the opposite directions.

Conclusions

The genome-wide SNPs revealed seven gene pools and fine spatial genetic structure of the Ae. albopictus population in China. The RAD-seq approach has great potential to increase our understanding of the spatial dynamics of population spread and establishment, which will help us to design new strategies for controlling vectors and mosquito-borne diseases.