bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolated Intramural Hematoma of Superior Mesenteric Artery: Case Reports and a Review of Literature
Authors: Ascione Marta , Cangiano Rocco , Mohseni Alireza , Molinari Andrea , Marzano Antonio , Di Girolamo Alessia , Di Marzo Luca , Mansour Wassim
Published: December 2023

(1) Background: Spontaneous isolated intramural hematoma of the superior mesenteric artery (SIHSMA) is a rare entity often considered as a subset of spontaneous isolated dissection of the superior mesenteric artery (SIDSMA). It is characterized by a completely thrombosed false lumen with or without an ulcer-like projection with computed tomography (CT) imaging. The recent literature describes few reports with a relatively short-term follow-up. The natural course, prognosis, and treatment options for SIHSMA still lack consensus. We present two cases of acute abdominal pain in a young man due to IMH of the superior mesenteric artery with an extensive literature review. (2) Case report: A 46-year-old male patient was submitted to an urgent CTA for acute abdominal pain, showing the presence of an isolated dissection of the superior mesenteric artery, determining significant stenosis of the vessel with collateral vessel patency. The patient referred to a recent COVID-19 infection, whose course was paucisymptomatic. He was conservatively treated with antiplatelet therapy and corticosteroid treatments, and, after a few days, the symptomatology completely regressed; also, the 2-month-control CTA showed complete IMH regression and the absence of any signs of residual stenosis. The second patient was a 61-year-old male patient who was submitted to an urgent CTA for acute abdominal pain, showing the presence of an isolated dissection of the superior mesenteric artery, not determining significant vessel stenosis. He was conservatively treated with antiplatelet therapy and corticosteroid treatment, and after a few days, the symptomatology completely regressed and the radiological control showed complete dissection regression. (3) Conclusion: SISHSMA is a rare entity of vascular pathology, and conservative management represents the best medical strategy. We propose corticosteroid treatment as one of the most appropriate tools in the conservative treatment of SISHSMA.
Imaging Evaluation of Platelet-Rich Fibrin in Post-Exodontic Bone Regeneration: A Systematic Review
Authors: Molina-Barahona Magdalena , Delgado-Gaete Bolívar , Morales-Navarro Denia , Urbizo-Vélez Joaquín , Avecillas-Rodas Renata
Published: November 2023

Tooth extraction is the most common procedure in dental practice. However, in the long term, it may cause alveolar ridge atrophy. This systematic review aimed to evaluate the role of platelet-rich fibrin (PRF) in post-exodontic alveolar ridge preservation in terms of its effectiveness in the regeneration of bone tissue as assessed by imaging and its efficacy compared to physiological bone healing. The study is presented in accordance with the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. This systematic review was conducted using electronic databases such as PubMed, Scopus, Web of Science, and Science Direct. The gray literature search was conducted in the New York Academy of Medicine Grey Literature Report. All the studies in this systematic review were randomized controlled trials (RCTs). The risk of bias was performed according to the Cochrane Handbook for Systematic Reviews of Interventions 6.2 (RevMan 6.2). Considering the inclusion and exclusion criteria, we included 17 randomized clinical trials published up to 2022 investigating the efficacy of PRF in post-exodontic bone regeneration. Based on the results of clinical studies, it can be stated that despite not being statistically significant, PRF promotes neoformation and prevents bone loss between three and four months post-extraction.
The Impact of Geopolitical Risk on Portuguese Exports
Authors: Leitão Nuno Carlos
Published: December 2023

This investigation evaluates the performance of Portuguese exports by focusing on the 11 main partners for 1990–2021, considering panel data. Country risk analysis has been frequently used to assess the determinants of international trade in recent years. Empirical studies demonstrate that country risk can affect bilateral relationships between economies, especially in economies with greater geopolitical risk. Next, we refer to the methodology used in this research. In this context, we assessed the stationarity of the variables used in this study. Subsequently, models were used to eliminate bias and endogeneity between the variables. The panel quantile regression model allows us to understand the behaviour of variables across different quartiles. The empirical study shows that countries with low country risk promote the performance of Portuguese exports. On the other hand, the size of the economies, both the exporting country (Portugal) and the importing countries (commercial partners), is decisive for increasing Portuguese exports. This finding can be explained as a monopolistic competition with the economy’s scale and industrial concentration serving as theoretical support. As noted with previous studies on the gravity model, the common language of Portuguese-speaking countries reduces communication costs and increases Portuguese exports. Furthermore, the econometric model also validates the issue of geographical distance, where this variable has a negative impact on exports, demonstrating that geographical proximity reduces transport costs.
Brain Science and Geographic Thinking: A Review and Research Agenda for K-3 Geography
Authors: Gersmehl Phil
Published: November 2023

How does a child learn to read a map? In 2007, the authors of an article in the Journal of Geography proposed a tentative list of eight “modes of spatial reasoning” that children may use to organize their perceptions of information on a map. As an update, this article has short descriptions of these modes, brief reviews of research since 2007, and some suggestions of topics for future investigation. This article includes a brief look at some implications for teaching math and reading, followed by an extended report about a classroom activity that underscores the main point about the parallel perception and processing of different kinds of spatial information. A technical appendix has a more detailed summary of the process used to identify and classify the modes of spatial reasoning.
Social Support and Its Impact on Job Satisfaction and Emotional Exhaustion
Authors: Garmendia Pablo , Fernández-Salinero Samuel , Holgueras González Ana Isabel , Topa Gabriela
Published: December 2023

Social support at work has demonstrated itself to be an important variable for predicting desirable outcomes and helping to buffer the effects of adverse events. The main objective of this research is to understand the impact of social support on job satisfaction on the one hand and emotional exhaustion on the other. Furthermore, in order to gain a deeper understanding of intricate organizational relationships, the mediating effects of work recovery experiences are taken into consideration. The sample was composed of 496 workers (41.5% men and 58.5% women). The mean age was 42 years (SD = 9.82). A cross-sectional design was used. The results, both direct (r = 0.43; R2 = 0.19; p < 0.001) and indirect (B = 0.04; SE = 0.02; 95% C.I. = 0.01, 0.09), of the model relating social support to job satisfaction were statistically significant. On the other hand, in the model that links social support to emotional exhaustion, we observed statistically significant direct (r = 0.26; R2 = 0.07; p < 0.001) and indirect effects (B = −0.05; SE = 0.02; 95% C.I. = −0.10, −0.01). Only the relaxation factor was a significant mediator of these variables. Implications, limitations, and future research recommendations are discussed.
A Scoping Review of Potential Biological Mechanisms and Predictors of Interpersonal Psychotherapy
Authors: Papke Victoria , Hodges Hopewell , Reigstad Kristina , Gunlicks-Stoessel Meredith , Klimes-Dougan Bonnie
Published: December 2023

Social dysfunction plays a critical role in the development and maintenance of depression in both adolescents and adults. Interpersonal psychotherapy (IPT) and interpersonal psychotherapy for depressed adolescents (IPT-A) are effective, evidence-based, and time-limited treatments for depression that aim to mitigate depressive symptoms by strengthening an individual’s interpersonal relationships and skills. Though the efficacy of IPT/IPT-A has been well established, we are just beginning to know how biological systems are implicated in its success. In this scoping review, we examine the extant literature on biological mechanisms and predictors of IPT/IPT-A treatment efficacy. Overall, seven studies were identified that consider biological processes in the context of evaluating IPT/IPTA, and the studies that were conducted are typically preliminary in nature. Notably, there is some evidence showing that the hypothalamic–pituitary–adrenal axis, various frontal and limbic brain regions, and behavioral indexes that represent brain functioning are associated with changes in IPT/IPT-A or predictive of IPT/IPT-A outcomes. We also consider consequences for treatment and future research. The hope is that a better understanding of how and for whom IPT/IPT-A works can optimize the success of the treatment in reducing an individual’s depressive symptoms.
Numerical Investigation of the Seismic Performance of an Innovative Type of Buckling-Restrained Brace (BRB)
Authors: Naghshineh Ali , Fischer Oliver , Pathan Nasreen B. , Couch Logan , Tehrani Fariborz M.
Published: December 2023

Previous studies have demonstrated that the inclusion of tire-derived aggregate (TDA) enhances the damping, ductility, and toughness of concrete mixtures. The effectiveness of tire-derived aggregate as a ductile material with a higher damping ratio and lower density in buckling-restrained braces has been examined at California State University’s Structures Laboratory (CSU). Through experimental and theoretical investigations, this study compares the structural application of buckling-restrained braces with TDA and with conventional concrete infill subjected to various ground motions as well as artificial excitations. The evaluations include modeling a full-scale experimental setup equipped with a single-leg BRB utilizing ETABS 2016 and OpenSees 2000 software. The effectiveness of the application is demonstrated through a comparison of accelerations, displacements, stiffness, and damping ratios between TDA and concrete filling. Additionally, a design guideline for TDA-filled buckling-restrained braced frames is provided.
Effect of High-Pressure Micro-Fluidization on the Inactivation of Staphylococcus aureus in Liquid Food
Authors: Zhang Zequn , Cui Tianlin , Tai Luyang , Mu Kangyi , Shi Yicong , Chen Fang , Liao Xiaojun , Hu Xiaosong , Dong Li
Published: November 2023

High-pressure homogenization has been extensively studied for its excellent homogenization effect and the prospect of continuous liquid food production, but its sterilization ability still needs to be improved. In this study, we replaced the homogenization valve with two opposing diamond nozzles (0.05 mm inner diameter) so that the fluid collided at high velocity, corresponding to high-pressure micro-fluidization (HPM). Moreover, HPM treatment significantly inactivated Staphylococcus aureus ~7 log in the liquid with no detectable sub-lethal state at a pressure of 400 MPa and a discharge temperature of 50 °C. The sterilization effect of HPM on S. aureus subsp. aureus was attributed to a significantly disrupted cell structure and increased membrane permeability, which led to the leakage of intracellular proteins, resulting in bacterial death. At the same time, HPM treatment was able to significantly reduce the ability of S. aureus subsp. aureus to form biofilms, which, in turn, reduced its virulence. Finally, compared to the simulated system, more effective sterilization was observed in apple juice, with its color and pH remaining unchanged, which suggested that HPM can be used to process other liquid foods.
Insights into the Dynamic Succession of Microbial Community and Related Factors of Vanillin Content Change Based by High-Throughput Sequencing and Daqu Quality Drivers
Authors: Tong Wenhua , Wang Shuqin , Yang Ying , Huang Zhijiu , Li Yiyun , Huang Dan , Luo Huibo , Zhao Liming
Published: November 2023

Daqu is an important saccharifying starter in the fermentation of Nongxiangxing Baijiu in China. Vanillin is a health and flavor factor in Baijiu. However, only a few research studies on the vanillin content of Daqu are currently not systematic. In order to investigate the metabolic mechanism of vanillin in the fermentation process of Daqu, we analyzed the changes in microorganisms, influencing factors, and enzymes related to vanillin in Daqu. This research found that there were differences between bacterial and fungal genera in each sample, and the abundance of bacteria was greater than that of fungi. Among the microbial genera, Klebsiella, Escherichia, Acinetobacter, Saccharopolyspora, Aerococcus, and Puccinia were positively correlated with vanillin. Meanwhile, we also found that moisture and reducing sugar were the main physicochemical factors affecting the formation of vanillin. The functional annotation results indicate that carbohydrate metabolism and energy metabolism were important microbial metabolic pathways that impacted vanillin production in solid-state fermentation. The feruloyl-CoA hydratase/lyase (EC 4.1.2.61) and acylamidase (EC 3.5.1.4) were positively correlated with vanillin content (p ≤ 0.05) and promote the increase in vanillin content. These findings contribute to furthering our understanding of the functional microorganisms, physicochemical factors, and enzymes related to the change in vanillin content during the fermentation of Daqu and can help to further explore the flavor substances in Baijiu fermentation in the future.
US Consumers’ Awareness, Purchase Intent, and Willingness to Pay for Packaging That Reduces Household Food Waste
Authors: Fennell Korey , Lu Guanqi , Mahmoudi Monireh , Lee Euihark , Almenar Eva
Published: November 2023

Food waste is a barrier to the development of sustainable food systems, and a large portion of it occurs at the household level. Household food waste can be decreased by using appropriate packaging. Despite the high rate of food waste in US households, little is known about how packaging affects this. This study assessed US consumers’ awareness of how structural packaging designs and technologies affect food freshness and their willingness to purchase and to pay extra for packaging designed to reduce household food waste. To gather data, 1000 US consumers were surveyed online. Responses were analyzed overall and by population segments. The impacts of only 3 out of 15 structural packaging designs on maintaining food freshness were known by >50% of consumers. Regarding packaging technologies, while 78% of consumers knew about the impact of vacuum packaging on maintaining food freshness, just 27.6, 23, and 16% knew how modified atmosphere packaging, active packaging, and aseptic packaging affected food freshness. Only 32% of consumers knew that intelligent packaging provides information on food freshness. Just 9% of consumers recognized that foods in plastic pouches and cans possess the same food freshness. Approximately 91% of consumers will always/sometimes buy food in most of the above packaging technologies after learning about them. Half were willing to pay more for food in packages that reduce household food waste, and 40% may. Differences (p ≤ 0.05) and two-way interactions were observed between population segments. This study’s findings can help develop new packaging, education campaigns, and policies to reduce household food waste in the US.
Polymorphisms of CCSER1 Gene and Their Correlation with Milk Quality Traits in Gannan Yak (Bos grunniens)
Authors: Yang Guowu , Zhang Juanxiang , Ma Xiaoyong , Ma Rong , Shen Jinwei , Liu Modian , Yu Daoning , Feng Fen , Huang Chun , Ma Xiaoming , La Yongfu , Guo Xian , Yan Ping , Liang Chunnian
Published: November 2023

Coiled-coil serine-rich protein 1 (CCSER 1) gene is a regulatory protein gene. This gene has been reported to be associated with various economic traits in large mammals in recent years. The aim of this study was to investigate the association between CCSER1 gene single nucleotide polymorphisms (SNPs) and Gannan yaks and to identify potential molecular marker loci for breeding milk quality in Gannan yaks. We genotyped 172 Gannan yaks using Illumina Yak cGPS 7K liquid microarrays and analyzed the correlation between the three SNPs loci of the CCSER1 gene and the milk qualities of Gannan yaks, including milk fat, protein and casein. It was found that mutations at the g.183,843A>G, g.222,717C>G and g.388,723G>T loci all affected the fat, protein, casein and lactose traits of Gannan yak milk to varying extents, and that the milk quality of individuals with mutant phenotypes was significantly improved. Among them, the milk fat content of AG heterozygous genotype population at g.183,843A>G locus was significantly higher than that of AA and GG genotype populations (p < 0.05); the casein and protein content of mutant GG and CG genotype populations at g.222,717C>G locus was significantly higher than that of wild-type CC genotype population (p < 0.05); and the g.388,723G>T locus of the casein and protein contents of the mutant TT genotype population were significantly higher (p < 0.05) than those of the wild-type GG genotype population. These results provide potential molecular marker sites for Gannan yak breeding.
Novel Efficient Physical Technologies for Enhancing Freeze Drying of Fruits and Vegetables: A Review
Authors: Yao Jianhua , Chen Wenjuan , Fan Kai
Published: November 2023

Drying is the main technical means of fruit and vegetable processing and storage; freeze drying is one of the best dehydration processes for fruit and vegetables, and the quality of the final product obtained is the highest. The process is carried out under vacuum and at low temperatures, which inhibits enzymatic activity and the growth and multiplication of micro-organisms, and better preserves the nutrient content and flavor of the product. Despite its many advantages, freeze drying consumes approximately four to ten times more energy than hot-air drying, and is more costly, so freeze drying can be assisted by means of highly efficient physical fields. This paper reviews the definition, principles and steps of freeze drying, and introduces the application mechanisms of several efficient physical fields such as ultrasonic, microwave, infrared radiation and pulsed electric fields, as well as the application of efficient physical fields in the freeze drying of fruits and vegetables. The application of high efficiency physical fields with freeze drying can improve drying kinetics, increase drying rates and maintain maximum product quality, providing benefits in terms of energy, time and cost. Efficient physical field and freeze drying technologies can be well linked to sustainable deep processing of fruit and vegetables and have a wide range of development prospects.
Influence of Particle Size and Extraction Methods on Phenolic Content and Biological Activities of Pear Pomace
Authors: Ferreira Joana , Tkacz Karolina , Turkiewicz Igor Piotr , Santos Maria Isabel , Belas Adriana , Lima Ana , Wojdyło Aneta , Sousa Isabel
Published: November 2023

The main goal of this research was to investigate how particle size influences the characteristics of pear (Pyrus Communis L.) pomace flour and to examine the impact of different pre-treatment methods on the phenolic content and associated bioactivities. Pear pomace flour was fractionated into different particle sizes, namely 1 mm, 710 µm, 180 µm, 75 µm and 53 µm. Then two extraction methods, namely maceration with methanol and two-step extraction with hexane via Soxhlet followed by ultrasound extraction with methanol, were tested. Total phenolic and total flavonoid contents ranged from 375.0 to 512.9 mg gallic acid/100 g DW and from 24.7 to 34.6 mg quercetin/100 g DW, respectively. Two-step extraction provided antioxidant activity up to 418.8 (in FRAP assay) and 340.0 mg Trolox/100 g DW (in DPPH assay). In order to explore various bioactive properties, this study assessed the inhibitory effects of enzymes, specifically α-amylase and β-glucosidase (associated with antidiabetic effects), as well as angiotensin-converting enzyme (linked to potential antihypertensive benefits). Additionally, the research investigated antibacterial potential against both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria, revealing significant results (p < 0.05), particularly in the case of the two-step extraction method. This investigation underscores the substantial value of certain food industry wastes, highlighting their potential as bioactive ingredients within the framework of a circular economy.
Hemp Seed Cake Flour as a Source of Proteins, Minerals and Polyphenols and Its Impact on the Nutritional, Sensorial and Technological Quality of Bread
Authors: Capcanari Tatiana , Covaliov Eugenia , Negoița Cătălina , Siminiuc Rodica , Chirsanova Aurica , Reșitca Vladislav , Țurcanu Dinu
Published: November 2023

Hemp (Cannabis sativa L.) seeds contain a high concentration of proteins and biologically active compounds. The protein content is even higher in case of lipid part removal in oil production. The remaining part is considered a leftover, usually being used in animal feed. The aim of this study was to investigate the physicochemical composition of hemp seed cake flour, its nutritional quality and its impact on bread quality parameters. The properties of hemp seed cake flour were assessed in terms of protein quality, mineral composition, polyphenols and antioxidant activity. Hemp seed cake proved to be an important source of high-quality protein (31.62% d.m.) with the presence of eight essential amino acids. The biologically active potential of hemp seed cake has been demonstrated by the high content of polyphenols, especially those from the Cannabisin group. Hemp seed cake flour was incorporated in wheat flour at levels from 5 to 40% (w/w) to investigate its influence on bread quality parameters. The addition of hemp seed cake flour increased the total phenol content of bread, thus greatly enhancing the antioxidant activity. The protein content of bread was found to be enhanced from 11.11% d.m (control sample) to 18.18% d.m (for sample with 40% hemp seed cake flour). On the other hand, the addition of hemp seed cake flour led to decreased bread porosity, increased hardness and decreased resilience in the seed cake. Although, all bread samples recorded sensorial attributes ranging between “slightly like” and “like it very much”.
Overview of Pharmacological Therapies for Diffuse Tenosynovial Giant Cell Tumor
Authors: Stamatiou Antonia , Nguyen-Ngoc Tu , Wetterwald Laureline , Dolcan Ana-Maria , Dei Tos Giovanni , Cherix Stephane , Omoumi Patrick , Digklia Antonia
Published: December 2023

Tenosynovial giant cell tumor (TGCT) is a rare and locally aggressive benign tumor arising from the synovium of joints, bursae, and tendon sheaths. It is classified into localized (L-TGCT) and diffuse (D-TGCT) forms based on the extent of involvement. Surgical resection is the primary treatment, though achieving a definitive cure remains challenging due to the high recurrence rates, especially in D-TGCT. Systemic therapies targeting the CSF1-CSF1R axis have emerged as promising treatment options. CSF1R tyrosine kinase inhibitors (TKIs) such as imatinib, nilotinib, pexidartinib, and vimseltinib, alongside anti-CSF1R antibodies like emactuzumab, cabiralizumab, and lacnotuzumab, have shown encouraging results in managing TGCT, particularly when surgery is not feasible or poses significant morbidity. Other potential therapies, including local treatments and anti-inflammatory drugs, are being explored for TGCT management. This review provides an overview of systemic treatment options for D-TGCT, highlighting emerging therapeutic modalities and their potential implications. Effective management is crucial due to TGCT’s significant morbidity despite its non-life-threatening nature, necessitating novel approaches to improve patient prognosis and quality of life.
A Network of Sites and Upskilled Therapists to Deliver Best-Practice Stroke Rehabilitation of the Arm: Protocol for a Knowledge Translation Study
Authors: Carey Leeanne M. , Cahill Liana S. , Blennerhassett Jannette M. , Nilsson Michael , Lannin Natasha A. , Thijs Vincent , Hillier Susan , Cadilhac Dominique A. , Donnan Geoffrey A. , Morris Meg E. , Churilov Leonid , Walker Marion , Ramanathan Shanthi , Pollack Michael , May Esther , Cloud Geoffrey C. , McGowan Sharon , Wijeratne Tissa , Budge Marc , McKinnon Fiona , Olver John , Hogg Toni , Murray Michael , Haslam Brendon , Koukoulas Irene , Nielsen Brittni , Mak-Yuen Yvonne , Turville Megan , Neilson Cheryl , Butler Anna , Kim Joosup , Matyas Thomas A.
Published: December 2023

Implementation of evidence-informed rehabilitation of the upper limb is variable, and outcomes for stroke survivors are often suboptimal. We established a national partnership of clinicians, survivors of stroke, researchers, healthcare organizations, and policy makers to facilitate change. The objectives of this study are to increase access to best-evidence rehabilitation of the upper limb and improve outcomes for stroke survivors. This prospective pragmatic, knowledge translation study involves four new specialist therapy centers to deliver best-evidence upper-limb sensory rehabilitation (known as SENSe therapy) for survivors of stroke in the community. A knowledge-transfer intervention will be used to upskill therapists and guide implementation. Specialist centers will deliver SENSe therapy, an effective and recommended therapy, to stroke survivors in the community. Outcomes include number of successful deliveries of SENSe therapy by credentialled therapists; improved somatosensory function for stroke survivors; improved performance in self-selected activities, arm use, and quality of life; treatment fidelity and confidence to deliver therapy; and for future implementation, expert therapist effect and cost-effectiveness. In summary, we will determine the effect of a national partnership to increase access to evidence-based upper-limb sensory rehabilitation following stroke. If effective, this knowledge-transfer intervention could be used to optimize the delivery of other complex, evidence-based rehabilitation interventions.
Variables Associated with Attitudes toward Biodebridement Using Lucilia sericata Larvae in a Group of Nurses
Authors: Bazaliński Dariusz , Pytlak Kamila , Przybek-Mita Joanna , Szymańska Paulina , Wójcik Anna , Zymon Aneta , Sherman Ronald , Nguyen Albert , Sałacińska Izabela , Więch Paweł
Published: December 2023

Despite numerous studies and recommendations, the acceptance of treatments involving medicinal maggots in many clinics has been slow. Several factors may account for this, including the gender of nurses administering the treatment, their level of work experience, and their perceived level of personal stress. The aim of the study was to assess the impact of selected variables (gender, work experience, stress level) on the readiness of nurses to administer maggot debridement therapy (MDT), which is a form of biodebridement. The study population was a cohort of 290 wound care nurses providing specialist care for patients with chronic wounds. It was assumed that the identified variables may determine the implementation of larval therapy in everyday professional practice. A subsample of 35 men and 35 women was further analyzed to determine if gender, work experience, and/or personal stress levels were correlated with attitudes towards the utilization of maggots in biodebridement. Assessment tools included the Perceived Stress Scale (PSS-10) and the MDT 10 Perception Assessment Questionnaire, a protocol by which the subject ranked six wound photographs in order of repulsiveness and responded to questions regarding demographic variables, which include education and work experience. The visual perception of pictures of a wound with larvae is indirectly an indicator of the attitude towards larval therapy. Selection of the photograph with maggots on the wound as the most repulsive image was associated with a personal appraisal of not being ready to implement maggot therapy (chi-square = 8.430, p = 0.015). Low work experience (chi-square = 14.039, df = 4, p = 0.007), and low readiness for MDT (chi-square = 8.430, df = 2, p = 0.015) were also associated with unpreparedness to administer maggot therapy. Neither gender nor perceived stress level were exclusively associated with disgust for maggots or lack of readiness to implement MDT. Low professional experience and a deficit of knowledge in maggot therapy may negatively affect the readiness of nurses to administer biodebridement. Gender and personal stress levels do not affect nurses’ readiness to utilize larval therapy.
The Challenges in Managing Peripheral Arterial Disease Complications
Authors: Biscetti Federico , Flex Andrea
Published: December 2023

Peripheral arterial disease (PAD) afflicts millions of people across the globe, with the severe form often culminating in chronic limb-threatening ischemia (CLTI) [...]
Acidovorax citrulli Type IV Pili PilR Interacts with PilS and Regulates the Expression of the pilA Gene
Authors: Yang Yuwen , Ji Weiqin , Qiao Pei , Fei Nuoya , Yang Linlin , Guan Wei , Zhao Tingchang
Published: December 2023

Acidovorax citrulli can cause bacterial fruit blotch of watermelon, melon, and other cucurbits, and has the potential to cause severe economic losses to growers throughout the world. This article investigated the functions and interactions of the pilR and pilS genes, two important genes in bacterial type IV pili systems, in A. citrulli. For each gene, deletion mutants and complementary strains were constructed via homologous recombination, and their phenotypes were determined. The results showed that the absence of pilR and pilS could significantly reduce the pathogenicity and twitching motility of A. citrulli while increasing the swimming motility, biofilm formation, and in vitro growth. Conversely, complementary strains were no different than the wild-type strain. Using quantitative reverse transcription PCR and promoter activity assays, we confirmed that the deletion of pilR and pilS genes leads to a significant decrease in the transcription level of pilA. Meanwhile, three methods including yeast two-hybrid, glutathione S-transferase pull-down, and luciferase complementation imaging assays were used to verify the direct interaction between the PilR and PilS proteins. These findings revealed the biological function of the pilR and pilS and confirms their regulatory role on pilA.
Study of Molecular Biodiversity and Population Structure of Vitis vinifera L. ssp. vinifera on the Volcanic Island of El Hierro (Canary Islands, Spain) by Using Microsatellite Markers
Authors: Fort Francesca , Lin-Yang Qiying , Suárez-Abreu Luis Ricardo , Sancho-Galán Pau , Canals Joan Miquel , Zamora Fernando
Published: December 2023

El Hierro island is postulated as the most biodiverse of the archipelago. To verify this hypothesis, the 87 individuals collected throughout the island were genotyped with 20 SSRs. As a result of this study, 28 varieties were described, 6 of which were new (Uval piñero, Uvalero volcánico, Pinar negro, Seis de Carlos, Tesoro blanco, Uval negro), and the first rose sport of the local Canary Islands variety Bermejuela was also found. Fifteen errors were detected in total. Eleven varieties were identified that were unknown to the vine growers and twenty individuals with variations (mutations) were found, of which two had already been described in a previous prospection in Lanzarote Island (intra-varietal variability). From this study, it is also proposed to incorporate 33 new names into the world database, corresponding mostly to the individuals and variations described for the first time, which represents a lexicographic enrichment. Finally, the singularity of the population of vines adapted to El Hierro island is demonstrated, not only with respect to the population of Canary Islands vines, but also with respect to the world population. The biodiversity and uniqueness of El Hierro and the Canary Archipelago reaffirm the proposal that the Canary Islands should be considered a world biodiversity centre.
Assessing Groundwater Quality and Diagnosing Nitrate Pollution in the Sidi Allal Region: A GIS-Based Approach Utilizing the Groundwater Pollution Index
Authors: Al-Aizari Hefdhallah S. , Ghfar Ayman A. , Al-Aizari Ali R. , Al-Aizari Abdul-Jaleel M. , Moshab Mohamed Sheikh , Sillanpää Mika
Published: December 2023

Groundwater is a critical resource for various human activities, yet it faces contamination risks from agricultural, industrial, and domestic sources. This study aimed to evaluate groundwater in Morocco’s Sidi Allal region using the groundwater pollution index (GPI) and diagnose nitrate pollution. The study included 45 groundwater wells from the study area, and physicochemical parameters such as pH, electrical conductivity, cations, and anions were examined in the laboratory. The geographic information system (GIS) was used to determine the spatial distribution of groundwater quality parameters. The groundwater pollution index and nitrate pollution index (NPI) were determined. The inverse distance weighting method (IDW) was used to create a spatial distribution map. The results indicated that the calculated GPI values ranged from 0.856 to 7.416, with an average of 2.06. About 40% of groundwater samples were highly polluted and unsuitable for drinking. The NPI values ranged between −0.74 and 10.5, with an average of 5.1. About 64% of the total groundwater samples were considered highly polluted according to the NPI classification, suggesting that the groundwater was unsuitable for drinking purposes. The spatial distribution map revealed the availability of appropriate groundwater in the central area of the study area and inappropriate groundwater near the Esbou River and Nassour Canal. The findings of this study revealed high concentrations of nitrates in groundwater samples in the central part of the study area, indicating that this increase in nitrates may be due to intensive use of nitrogen fertilizers in agricultural activities and sewage waste.
Effect of Porosity and Injection Ratio on the Performance of Transpiration Cooling through Gyroids
Authors: Brimacombe Benjamin J. , Scobie James A. , Flynn Joseph M. , Sangan Carl M. , Pountney Oliver J.
Published: December 2023

This paper presents experimental measurements of adiabatic effectiveness for three transpiration cooling porosities (ϕ= 0.3, 0.4, and 0.5) constructed from gyroid lattice structures. To the authors’ knowledge, this is the first use of a Triply Periodic Minimal Surface (TPMS) function to produce transpiration test coupons of varying porosity. Polymer gyroid lattice structures were successfully printed using Stereolithography (SLA) down to ϕ= 0.3 for a print resolution of 25 microns and unit cell size of 2 mm. Cooling performance was measured in a small-scale wind tunnel. High-resolution Infrared Thermography was used to determine wall temperatures downstream of the porous section. When tested at both common blowing ratios (M = 0.029, 0.048, and 0.062) and common injection ratios (F = 0.010, 0.017, and 0.022) the cooling performance was found to be dependent on porosity for constant M but not for constant F. Having determined F as the more important parameter for comparison, results are presented alongside transpiration and effusion data from literature.
Preparing Enteral Formulas for Adult Patients with Phenylketonuria: A Minor Necessity but Major Challenge—A Case Report
Authors: Pané Adriana , Carrasco-Serrano Marcos , Milad Camila , Leyes Pere , Moreno-Lozano Pedro Juan , Ventura Roser , Milisenda José Cesar , García-García Francesc Josep , Garrabou Glòria , García-Villoria Judit , López-Galera Rosa Maria , Ribes Antonia , Grau-Junyent Josep Maria , Forga-Visa Maria de Talló , Montserrat-Carbonell Cristina ,   on behalf of PKU.CAT Consortium
Published: December 2023

Phenylketonuria (PKU) is the most frequent of the congenital errors of amino acid (AA) metabolism worldwide. It leads to the accumulation of the essential AA phenylalanine (Phe) and it is associated with severe neurological defects. The early diagnosis and treatment of this rare disease, achieved through newborn screening and low-Phe diet, has profoundly changed its clinical spectrum, resulting in normal cognitive development. We face the first generation of PKU patients perinatally diagnosed and treated who have reached adulthood, whose special needs must be addressed, including feeding through enteral nutrition (EN). However, recommendations regarding EN in PKU constitute a gap in the literature. Although protein substitutes for patients with PKU are offered in multiple forms (Phe-free L-amino acid or casein glycomacropeptide supplements), none of these commercial formulas ensures the whole provision of daily total energy and protein requirements, including a safe amount of Phe. Consequently, the combination of different products becomes necessary when artificial nutrition via tube feeding is required. Importantly, the composition of these specific formulas may result in physicochemical interactions when they are mixed with standard EN products, leading to enteral feeding tubes clogging, and also gastrointestinal concerns due to hyperosmolality. Herein, we present the first reported case of EN use in an adult patient with PKU, where the separate administration of protein substitutes and the other EN products avoided physicochemical interactions.
Comparison of Phaco-Tanito Microhook Trabeculotomy between Propensity-Score-Matched 120-Degree and 240-Degree Incision Groups
Authors: Sugihara Kazunobu , Shimada Ayaka , Ichioka Sho , Harano Akiko , Tanito Masaki
Published: December 2023

This study compared the effectiveness and safety of 120-degree (nasal) and 240-degree (bilateral) incisions in Tanito Microhook Trabeculotomy (TMH) combined with cataract surgery in patients with open-angle glaucoma. From a pool of 185 eyes, 67 eyes from 67 subjects were selected for each incision group using propensity score matching to align age, sex, glaucoma type, and preoperative intraocular pressure (IOP). The study found that preoperative IOP, initially 18.6 mmHg in both groups, decreased to 13.2 mmHg in the nasal group and 12.8 mmHg in the bilateral group 12 months postoperatively, representing reductions of 29% and 31%, respectively. Similarly, medication scores decreased from 3.4 to 2.7 in the nasal group and from 3.1 to 2.5 in the bilateral group. Notably, the bilateral incision group exhibited a significantly higher hyphema red blood cell score compared to the nasal group (p < 0.0001). Across the study period, other parameters such as IOP, medication score, visual acuity, anterior chamber flare, corneal endothelial cell density, visual field mean deviation, and the frequency of surgical complications other than hyphema were similar between the groups. The study concluded that TMH combined with cataract surgery is equally effective and safe regardless of incision width, although narrower incisions resulted in reduced early postoperative hyphema.
Comparison of Clinical Outcomes of Tracheostomy between COVID-19 and Non-COVID-19 Patients
Authors: Jung Sung Ha , Park Joo Hyun , Yi HeeJun , Kim Heejung , Lee Gil Joon , Choi Nayeon
Published: December 2023

Background and Objectives: We compared decannulation-related factors between COVID-19 and non-COVID-19 patients who underwent tracheostomy. Subjects and Methods: We conducted a retrospective study of patients who underwent a tracheostomy. The clinical factors were compared between the successful (decannulation within 3 months) and failed decannulation (decannulation over 3 months) groups in COVID-19 and non-COVID-19 patients. Results: The successful decannulation rates were 41.1% in COVID-19 and 45.1% in non-COVID-19 patients, with no significant differences in demographic and clinical factors between the two groups. In the non-COVID-19 patients, the failed decannulation group had a higher proportion of cerebrovascular and pulmonary diseases. Ventilator dependency or increased oxygen demand was the primary cause of decannulation failure in both groups, with no significant differences except for a higher prevalence of swallowing problems in the COVID-19 group (42.4% vs. 20.0%). Conclusions: The predominant cause of decannulation failure was ventilator and oxygen demand in both the non-COVID-19 and COVID-19 patients. In the non-COVID-19 patients, underlying cerebrovascular diseases were considered to have a significant impact on the decannulation process. On the other hand, swallowing problems significantly influenced decannulation among the COVID-19 patients. Therefore, we should consider early and active respiratory and swallowing rehabilitation to facilitate successful decannulation in COVID-19 patients.
Reply to Eker et al. Comment on “Kilian et al. Comparing Characteristics and Treatment of Brain Vascular Malformations in Children and Adults with HHT. J. Clin. Med. 2023, 12, 2704”
Authors: Kilian Alexandra , Latino Giuseppe A. , White Andrew J. , Ratjen Felix , McDonald Jamie , Whitehead Kevin J. , Gossage James R. , Krings Timo , Lawton Michael T. , Kim Helen , Faughnan Marie E. ,   The Brain Vascular Malformation Consortium HHT Investigator Group
Published: December 2023

We are grateful to Eker et al. for their thoughtful analysis and response to our publication titled Comparing Characteristics and Treatment of Brain Vascular Malformations in Children and Adults with HHT [...]
The Fate of Pre-Existing L5-S1 Degeneration following Oblique Lumbar Interbody Fusion of L4-L5 and Above
Authors: Kang Dong-Ho , Kim Ji Hwan , Chang Bong-Soon , Kim Hyoungmin , Kim Dongook , Park Sanghyun , Hong Seong Hwa , Chang Sam Yeol
Published: December 2023

Background: Previous studies have identified various risk factors for adjacent segment disease (ASD) at the L5-S1 level after fusion surgery, including preoperative sagittal imbalance, longer fusion, and preoperative disc degeneration. However, only a few studies have explored the risk factors for ASD at the L5-S1 level after oblique lumbar interbody fusion (OLIF) at the L4-L5 level and above. This study aimed to identify the risk factors for symptomatic ASD at the L5-S1 level in patients with pre-existing degeneration after OLIF at L4-L5 and above. Methods: We retrospectively reviewed the data of patients who underwent OLIF at L4-L5 and above, with a minimum follow-up period of 2 years. Patients with central stenosis or Lee grade 2 or 3 foraminal stenosis at L5-S1 preoperatively were excluded. Patients were divided into ASD and non-ASD groups based on the occurrence of new-onset L5 or S1 radicular pain requiring epidural steroid injection (ESI). The clinical and radiological factors were analyzed. Logistic regression was used to identify the risk factors for ASD of L5-S1. Results: A total of 191 patients with a mean age ± standard deviation of 68.6 ± 8.3 years were included. Thirty-four (21.7%) patients underwent ESI at the L5 root after OLIF. In the logistic regression analyses, severe disc degeneration (OR (95% confidence interval (CI)): 2.65 (1.16–6.09)), the presence of facet effusion (OR (95% CI): 2.55 (1.05–6.23)), and severe paraspinal muscle fatty degeneration (OR (95% CI): 4.47 (1.53–13.05)) were significant risk factors for ASD in L5-S1. Conclusions: In this study, the presence of facet effusion, severe disc degeneration, and severe paraspinal muscle fatty degeneration at the L5-S1 level were associated with the development of ASD at L5-S1 following OLIF at L4-L5 and above. For patients with these conditions, surgeons could consider including L5-S1 in the fusion when considering OLIF at the L4-L5 level and above.
The Effectiveness of Ultrasound-Guided, Continuous, Bilateral Erector Spinae Plane Block in Perioperative Pain Management of Patients Undergoing Colorectal Surgery: A Randomized, Controlled, Double Blind, Prospective Trial
Authors: Sifaki Freideriki , Vogiatzaki Theodosia , Mantzoros Ioannis , Koraki Eleni , Christidis Panagiotis , Pramateftakis Manousos-Georgios , Tsapara Vaia , Bagntasarian Stella , Ioannidis Orestis , Chloropoulou Pelagia-Paraskevi
Published: December 2023

Open and laparoscopic colorectal surgeries, while essential in the management of various colorectal pathologies, are associated with significant postoperative pain. Effective perioperative pain management strategies remain an anesthesiologic challenge. The erector spinae plane block (ESPB), a novel peripheral nerve block, has gained attention for its potential in providing analgesia for a wide variety of surgeries. This study aimed to evaluate the effectiveness of continuous, bilateral ultrasound-guided ESPB in perioperative pain management of patients undergoing colectomy. This prospective, randomized, controlled, double-blind trial included 40 adult patients scheduled for elective open or laparoscopic colectomy. Patients undergoing open colectomy as well as patients undergoing laparoscopic colectomy were randomly allocated into two groups: the ESPB group (n = 20) and the control group (n = 20). All patients received preoperatively ultrasound-guided, bilateral ESPB with placement of catheters for continuous infusion. Patients in the ESPB group received 0.375% ropivacaine, while patients in the control group received sham blocks. All patients received standardized general anesthesia and multimodal postoperative analgesia. Pain scores, perioperative opioid consumption, and perioperative outcomes were assessed. Patients in the ESPB group required significantly less intraoperative (p < 0.001 for open colectomies, p = 0.002 for laparoscopic colectomies) and postoperative opioids (p < 0.001 for open colectomies, p = 0.002 for laparoscopic colectomies) and had higher quality of recovery scores on the third postoperative day (p = 0.002 for open and laparoscopic colectomies). Patients in the ESPB group did not exhibit lower postoperative pain scores compared to those in the control group (p > 0.05 at various time points), while patients in both groups reported comparable satisfaction scores with their perioperative pain management (p = 0.061 for open colectomies, and p = 0.078 in laparoscopic colectomies). No complications were reported. ESPB is a novel and effective strategy in reducing perioperative opioid consumption in patients undergoing colectomy. This technique, as part of a multimodal analgesic plan and enhanced recovery after surgery protocols, can be proven valuable in improving the comfort and satisfaction of patients undergoing colorectal surgery.