bluuebunny/crossref_metadata_2025 huggingface dataset:
RGB-Based Triple-Dual-Path Recurrent Network for Underwater Image Dehazing
Authors: Alenezi Fayadh
Published: September 2022

In this paper, we present a powerful underwater image dehazing technique that exploits two image characteristics—RGB color channels and image features. In using RGB color channels, each color channel is decomposed into two units based on the similarities via the k-mean. This markedly improves the adaptability and identification of similar pixels, and thus reduces pixels with a weak correlation, leaving only pixels with a higher correlation. We use an infinite impulse response (IIR) in the triple-dual and parallel interaction structure to suppress hazed pixels via a pixel comparison and amplification to increase the visibility of even very minor features. This improves the visual perception of the final image, thus improving the overall usefulness and quality of the image. The softmax-weighted fusion is finally used to fuse the output color channel features to attain the final image. This preserves the color, leaving our proposed method’s output very true to the original scene’s. This is accomplished by taking advantage of adaptive learning based on the confidence levels of the pixel contribution variation in each color channel during subsequent fuses. The proposed technique both visually and objectively outperforms the existing methods in several rigorous tests.
A Low BER DB-PAM4 Adaptive Equalizer for Large Channel Loss in Wireline Receivers
Authors: Wang Zheng , Lai Mingche , Lyu Fangxu , Hu Xiaoyue
Published: September 2022

A high-speed serial interface is the core IP of a high-performance computer, data center and interconnection network; its bandwidth and bit error performance restrict the development of the system. With the evolution of high-speed serial interface line rates from 56 G to 112 G in high-end information systems, their bit errors increase sharply, limiting system performance. In order to solve the high-bit-error problem of the 112 Gb/s high-speed serial interface, this paper proposes a low-error Duo-binary PAM4 (DB-PAM) receive equalization technology. This technology utilizes the Duo-binary (DB) signal in which the channel and the transceiving equalizer work together to realize the low-error reception of the 112 Gb/s signal in the high-attenuation channel. To solve the problem of difficulty in generating high-speed DB-PAM4 signals and the complex adjustment of equalization parameters, this paper proposes a two-step progressive equalization technique. In the first step, the technology transmits not-return-to-zero (NRZ) signals at the transmitter (TX) and generates a Duo-binary (DB) signal path at the receiver using the least mean square error (LMS) algorithm. In the second step, the technology sends a precode-PAM4 (pre-PAM4) signal at TX; at the receiving end, the adaptive equalization algorithm is used to adjust the DSP equalization parameters to generate the optimal equalization parameters of the DB-PAM4 signal. This paper uses Cadence’s AMS simulation platform to verify the receive equalizer of DB-PAM4. Simulation results show: when a 112 Gb/s pre-PAM4 signal passes through the 35 dB@28 GHz channel, the receiver (RX) utilizes the adaptive equalizer to generate a 112 Gb/s DB-PAM4 signal, and the receiver bit error rate (BER) is less than 3e-9.
Reliability of Wide Band Gap Power Electronic Semiconductor and Packaging: A Review
Authors: Wang Yalin , Ding Yi , Yin Yi
Published: September 2022

Wide band gap (WBG) power electronic devices, such as silicon carbide metal–oxide–semiconductor field-effect transistors (SiC MOSFETs) and gallium–nitride high-electron-mobility transistors (GaN HEMTs) have been widely used in various fields and occupied a certain share of the market with rapid momentum, owing to their excellent electrical, mechanical, and thermal properties. The reliability of WBG power electronic devices is inseparable from the reliability of power electronic systems and is a significant concern for the industry and for academia. This review attempts to summarize the recent progress in the failure mechanisms of WBG power electronic semiconductor chips, the reliability of WBG power electronic packaging, and the reliability models for predicting the remaining life of WBG devices. Firstly, the typical structures and dominant failure mechanisms of SiC MOSFETs and GaN HEMTs are discussed. This is followed by a description of power electronic packaging failure mechanisms and available packaging materials for WBG power electronic devices. In addition, the reliability models based on physics-of-failure (including time-dependent dielectric breakdown models, stress–strain models, and thermal cycling models), and data-driven models are introduced. This review may provide useful references for the reliability research of WBG power devices.
Sustainability Impact Assessment of Forest Bioenergy Value Chains in Quebec (Canada)—A ToSIA Approach
Authors: Locoh Ayaovi , Thiffault Évelyne , Barnabé Simon
Published: September 2022

Forest bioenergy value chains can offer attractive opportunities to promote economic development and mitigate climate change. However, implementing profitable and efficient forest biomass value chains requires overcoming barriers that continue to hinder the development of bioenergy systems in several jurisdictions. The objective of this study was to compare the economic, social, and environmental sustainability of various potential configurations of forest bioenergy value chains, including forest biomass supply and bioenergy production chains, in the Capitale-Nationale region of Quebec (Canada), which is a jurisdiction that has considerable forest resources but makes little use of bioenergy. We based our study on the ToSIA model parameterization and compared various policy measures, biomass supply, and logistics scenarios for 2008 and 2030. Our results showed that wood chip and pellet value chains in the Capitale-Nationale region would positively contribute to the regional economy in 2030, even in the absence of subsidies. Moreover, actions to increase biomass feedstock mobilization in 2030 would lead to an increase in gross value added, employment, and energy production in the region compared with 2008 and a greater increase than other considered policy or logistical measures. However, increased biomass feedstock mobilization would also mean higher relative GHG emissions and more fossil fuel energy input per unit of bioenergy than in the other scenarios. Conversely, optimizing biomass feedstock and combustion technologies could help minimize the fossil fuel energy input needed and GHG and some non-GHG pollutant emissions. Overall, our study suggested that implementing policy and logistical measures for forest biomass value chains could make the significant mobilization of forest bioenergy attainable and, in turn, Quebec’s 2030 bioenergy target of 17 petajoules realistic.
A Method for Evaluating the Rock Breaking Efficiency of Cutters and Optimizing the PDC Cutter Profile—A Study of Igneous Rock Formations in Shunbei Oilfield
Authors: Dong Zhuoxin , Zhang Hui , Li Jun , Zhang Kuangsheng , Ou Yangyong , Lu Zongyu , Shi Jiangang
Published: September 2022

The Permian igneous rock in Shunbei Oilfield exhibits high rock strength, which results in a low rate of penetration (ROP) and shortens the cutter’s service life. It is necessary to analyze and evaluate the rock breaking effect of cutters. However, at this stage, the evaluation of the rock breaking effect has been limited to comparing the sizes of the mechanical specific energy (MSE), and the change in the rock breaking efficiency caused by the difference in the shape of the cutters’ surface has not been considered. Therefore, through the establishment of numerical simulation models of a circular cutter, bevel cutter, axe cutter, wedge cutter, and triangular cutter, the evaluation of the rock breaking efficiency of special-shaped cutters was completed. The results show that the triangular cutter and the wedge cutter are suitable for the front row cutter of the polycrystalline diamond compact bit (PDC); the triangular cutter is suitable for drilling into medium–hard formations, the wedge cutter is suitable for drilling into hard formations, and the bevel cutter is suitable for the back row cutter of the PDC, to assist other cutters in the process of rock breaking. The research results can provide the basis for the selection of PDC bit cutters and the design optimization of the bit.
Scenario Analysis of an Electric Power System in Colombia Considering the El Niño Phenomenon and the Inclusion of Renewable Energies
Authors: Restrepo-Trujillo Juliana , Moreno-Chuquen Ricardo , Jiménez-García Francy , Flores Wilfredo , Chamorro Harold
Published: September 2022

This paper develops and analyzes four energy scenarios for Colombia that consider the El Niño phenomenon and the inclusion of renewable energies in the energy generation matrix for the period 2020–2035. A comparative analysis is presented between the results of the different scenarios proposed. The most relevant finding is the use of the reserve margin as an indicator of system reliability. A scenario which included 7214 MW of large-scale non-conventional renewable energy, 10,000 MW of distributed generation, and 12,240 MW of hydroelectric power was assumed, with a reserve margin of over 50%. Additionally, it was found that for the scenarios in which a generation capacity with non-conventional renewable energies of less than 10,000 MW in 2034 was assumed, the reserve margin of the system in the seasons of the El Niño phenomenon will be less than historical records of the system. Alternatively, it was found that the scenarios in which the inclusion of at least 9600 MW of the electric power generation capacity of non-conventional renewable energies proposed by 2034 offer benefits in the reduction in greenhouse gas (GHG) emissions, which contributes to the achievement of the emission reduction objectives of the Paris Agreement.
Unsteady Effects of Wake on Downstream Rotor at Low Reynolds Numbers
Authors: Li Lin , Tan Wei , Zhang Jianshe , Han Ge , Zhang Yanfeng
Published: September 2022

In a compressor, the periodic wake is an inherently unsteady phenomenon that affects the downstream flow conditions and loading distribution. Thus, understanding the physical mechanisms of these unsteady effects is important for eliminating flow losses and improving compressor performance, particularly at low Reynolds numbers. To understand the influence of the upstream wake on the downstream flow field structure, this paper describes numerical simulations of a one-stage high-pressure compressor at altitudes of 10–20 km. The influence of the wake on rotor flow blockage at different Reynolds numbers is analyzed, and the unsteady interaction between the upstream wake and boundary layer or tip leakage flow is discussed. The results indicate that the wake has a beneficial effect on the efficiency of the rotor at high Reynolds numbers, but this weakens and becomes negative as the Reynolds number decreases. The wake can reduce the flow blockage in the mainflow region. Due to the wake, the length of the laminar separation bubble at high Reynolds numbers decreases and that at low Reynolds numbers increases. In addition, the unsteadiness of the wake causes separation bubbles to appear periodically at high Reynolds numbers and induces an open separation bubble at low Reynolds numbers. The Kelvin–Helmholtz instability can dominate the transition process of the boundary layer, which is also affected by the disturbance vortex induced by the wake. Regarding the tip leakage flow, the wake can reduce the flow blockage at high Reynolds numbers but increase the flow blockage at low Reynolds numbers. The interaction at low Reynolds numbers causes a double-leakage flow, which finally leads to the large-scale separation of the suction surface boundary layer. The large-scale separation causes flow blockage in the tip region and prevents the rotor wake from propagating downstream. On the contrary, the unsteady wake can pass through the tip clearance vortex and inhibit the separation of the suction boundary layer at high Reynolds numbers, which is reflected in a larger amplitude of one blade passage frequency. Therefore, the flow loss in the downstream flow field at high Reynolds numbers is significantly reduced at high Reynolds numbers.
Optimising Photovoltaic Farm Location Using a Capabilities Matrix and GIS
Authors: Kowalczyk Anna Maria , Czyża Szymon
Published: September 2022

Renewable energy sources provide an important solution in environmental protection activities and in the process of shaping sustainable development. The search for optimal locations enabling full exploitation of the energy intensity of real estate presents a significant challenge in terms of geoinformation analysis methods in a GIS environment. The aim of the study was to develop a capabilities matrix for the location of photovoltaic farms and, based on this, to compile a map of decision alternatives for these locations. The first stage involved the determination of the spatial features (stimulants and destimulants), which were significant in the context of photovoltaic (PV) farm location. During the analysis, the scope of the necessary data and their sources, which included topographic vector studies, aerial images, and the OpenStreetMap open data, were determined. The next stage was to determine the weights of the features which were significant in the context of photovoltaic (PV) farm location. To this end, the Multicriteria Decision Making (MCDM) method, including the Analytic Hierarchy Process (AHP) method, was employed. For the verification of the results, the entropy measure was also used. Entropy was calculated based on the diversity of previously identified geospatial features that shape the optimum conditions for their location, based on the photovoltaic farms already existing in Poland. A total of 555 photovoltaic farms were analysed. The next stage assumed the performance of geoinformation analyses using GIS tools and the development of a capabilities matrix for the PV farm location for the selected commune in Poland. The final stage involved the compilation of a PV decision alternative map for the selected commune based on the capabilities matrix. As a result, as an example, a ranking of plots was developed as decision-making alternatives for the municipality of Czarnia located in the northeastern part of Poland. It shows which parcels of land primarily have the dimension of spatial features that are favourable for the location of PV. More than 6900 parcels were analysed, in which 176 presented the highest value of the index of optimal PV location generated using the AHP method. This method provides a basis for further work by identifying optimal locations taking into account existing spatial conditions. The analyses carried out can be an important document in the future for spatial management, in particular for the location of new PV farms. As a continuation of the research, the authors will work on expanding the scope of the analyses and automating the entire process.
Modeling and Control Strategy of Wind Energy Conversion System with Grid-Connected Doubly-Fed Induction Generator
Authors: Chhipą Abrar Ahmed , Chakrabarti Prąsun , Bolshev Vadim , Chakrabarti Tulika , Samarin Gennady , Vasilyev Alexey N. , Ghosh Sandeep , Kudryavtsev Alexander
Published: September 2022

The most prominent and rapidly increasing source of electrical power generation, wind energy conversion systems (WECS), can significantly improve the situation with regard to remote communities’ power supply. The main constituting elements of a WECS are a wind turbine, a mechanical transmission system, a doubly-fed induction generator (DFIG), a rotor side converter (RSC), a common DC-link capacitor, and a grid-side converter. Vector control is center for RSC and GSC control techniques. Because of direct and quadrature components, the active and reactive power can also be controller precisely. This study tracks the maximum power point (MPP) using a maximum power point tracking (MPPT) controller strategy. The MPPT technique provides a voltage reference to control the maximum power conversion at the turbine end. The performance and efficiency of the suggested control strategy are validated by WECS simulation under fluctuating wind speed. The MATLAB/Simulink environment using simpower system toolbox is used to simulate the proposed control strategy. The results reveal the effectiveness of the proposed control strategy under fluctuating wind speed and provides good dynamic performance. The total harmonic distortions are also within the IEEE 519 standard’s permissible limits which is also an advantage of the proposed control approach.
Interleukin-17 Family Cytokines in Metabolic Disorders and Cancer
Authors: Meehan Eileen Victoria , Wang Kepeng
Published: September 2022

Interleukin-17 (IL-17) family cytokines are potent drivers of inflammatory responses. Although IL-17 was originally identified as a cytokine that induces protective effects against bacterial and fungal infections, IL-17 can also promote chronic inflammation in a number of autoimmune diseases. Research in the last decade has also elucidated critical roles of IL-17 during cancer development and treatment. Intriguingly, IL-17 seems to play a role in the risk of cancers that are associated with metabolic disorders. In this review, we summarize our current knowledge on the biochemical basis of IL-17 signaling, IL-17′s involvement in cancers and metabolic disorders, and postulate how IL-17 family cytokines may serve as a bridge between these two types of diseases.
Research on the Dynamic Evolution of the Landscape Pattern in the Urban Fringe Area of Wuhan from 2000 to 2020
Authors: Long Yan , Luo Shiqi , Liu Xi , Luo Tianyue , Liu Xuejun
Published: September 2022

The urban fringe area is a discontinuous spatial phenomenon that refers to the urban-rural interlacing zone which is undergoing urbanization on the fringe of the core built-up area of a large city after the emergence of industrialization. Dynamic, ambiguous, and complex interlacing of various types of lands make urban planners and managers fuzzy about the spatial scope of the urban fringe and it is difficult to control its evolution patterns scientifically. Based on remote sensing data from 2000 to 2020, the range of Wuhan’s urban fringe was extracted from the surface impermeability ratio mutation points, landscape flocculation, and population density. On this basis, the dynamic evolution characteristics of land-use and landscape patterns in the urban fringe area of Wuhan City were analyzed by using dynamic change and landscape pattern index analysis. The results show that: Wuhan City shows a clear “urban core area-urban fringe area-rural hinterland” circle structure, and the urban fringe area continuously extends to the rural hinterland. Moreover, most of the rural hinterland, in the process of moving to the urban core area, has gone through the process of the urban fringe. By comparison with other cities, it is found that the expansion of large cities is generally influenced by policies, topography, and traffic arteries, and gradually shifts from expansion to infill, with the urban core of Wuhan continuously extending and the urban fringe rapidly expanding from 2000 to 2010, and gradually entering a stable development state from 2010 to 2020. The future urban construction of Wuhan should pay attention to the influences of these characteristics on the implementation of urban territorial spatial planning.
Nanotechnology Meets Oncology: A Perspective on the Role of the Personalized Nanoparticle-Protein Corona in the Development of Technologies for Pancreatic Cancer Detection
Authors: Caputo Damiano , Quagliarini Erica , Pozzi Daniela , Caracciolo Giulio
Published: September 2022

In recent years nanotechnology has opened exciting opportunities in the struggle against cancer. In 2007 Dawson and coworkers demonstrated that nanomaterials exposed to biological fluids are coated with plasma proteins that form the so-called “protein corona”. A few years later our joint research team made of physicists, chemists, biotechnologists, surgeons, oncologists, and bioinformaticians introduced the concept of “personalized protein corona” and demonstrated that it is unique for each human condition. This concept paved the way for the development of nano-enabled blood (NEB) tests for the diagnosis of pancreatic ductal adenocarcinoma (PDAC). These studies gave an impetus to serious work in the field that came to maturity in the late 2010s. In this special issue, we provide the reader with a comprehensive overview of the most significant discoveries of our research team in the field of PDAC detection. We focus on the main achievements with an emphasis on the fundamental aspects of this arena and how they shaped the integration of different scientific backgrounds towards the development of advanced diagnostic technologies. We conclude the review by outlining future perspectives and opportunities to transform the NEB tests into a reliable clinical diagnostic technology for early diagnosis, follow-up, and management of PDAC patients.
Integrated Analysis of Transcriptome and Small RNAome Reveals the Regulatory Network for Rapid Growth in Mikania micrantha
Authors: Mo Xiaowei , Chen Haolang , Yang Xiaolan , Mo Beixin , Gao Lei , Yu Yu
Published: September 2022

M. micrantha has caused huge ecological damage and economic losses worldwide due to its rapid growth and serious invasion. However, the underlying molecular mechanisms of its rapid growth and environmental adaption remain unclear. Here, we performed transcriptome and small RNA sequencing with five tissues of M. micrantha to dissect miRNA-mediated regulation in M. micrantha. WGCNA and GO enrichment analysis of transcriptome identified the gene association patterns and potential key regulatory genes for plant growth in each tissue. The genes highly correlated with leaf and stem tissues were mainly involved in the chlorophyll synthesis, response to auxin, the CAM pathway and other photosynthesis-related processes, which promoted the fast growth of M. micrantha. Importantly, we identified 350 conserved and 192 novel miRNAs, many of which displayed differential expression patterns among tissues. PsRNA target prediction analysis uncovered target genes of both conserved and novel miRNAs, including GRFs and TCPs, which were essential for plant growth and development. Further analysis revealed that miRNAs contributed to the regulation of tissue-specific gene expression in M. micrantha, such as mmi-miR396 and mmi-miR319. Taken together, our study uncovered the miRNA-mRNA regulatory networks and the potential vital roles of miRNAs in modulating the rapid growth of M. micrantha.
MC180295 Inhibited Epstein–Barr Virus-Associated Gastric Carcinoma Cell Growth by Suppressing DNA Repair and the Cell Cycle
Authors: Fujii Tomohiro , Nishikawa Jun , Fukuda Soichiro , Kubota Naoto , Nojima Junzo , Fujisawa Koichi , Ogawa Ryo , Goto Atsushi , Hamabe Koichi , Hashimoto Shinichi , Wai Aung Phyo , Iizasa Hisashi , Yoshiyama Hironori , Sakai Kohei , Suehiro Yutaka , Yamasaki Takahiro , Takami Taro
Published: September 2022

DNA methylation of both viral and host DNA is one of the major mechanisms involved in the development of Epstein–Barr virus-associated gastric carcinoma (EBVaGC); thus, epigenetic treatment using demethylating agents would seem to be promising. We have verified the effect of MC180295, which was discovered by screening for demethylating agents. MC180295 inhibited cell growth of the EBVaGC cell lines YCCEL1 and SNU719 in a dose-dependent manner. In a cell cycle analysis, growth arrest and apoptosis were observed in both YCCEL1 and SNU719 cells treated with MC180295. MKN28 cells infected with EBV were sensitive to MC180295 and showed more significant inhibition of cell growth compared to controls without EBV infection. Serial analysis of gene expression analysis showed the expression of genes belonging to the role of BRCA1 in DNA damage response and cell cycle control chromosomal replication to be significantly reduced after MC180295 treatment. We confirmed with quantitative PCR that the expression levels of BRCA2, FANCM, RAD51, TOP2A, and CDC45 were significantly decreased by MC180295. LMP1 and BZLF1 are EBV genes with expression that is epigenetically regulated, and MC180295 could up-regulate their expression. In conclusion, MC180295 inhibited the growth of EBVaGC cells by suppressing DNA repair and the cell cycle.
Impact of Acetate in Reduction of Perchlorate by Mixed Microbial Culture under the Influence of Nitrate and Sulfate
Authors: Yu Hosung , Lee Kang Hoon , Park Jae-Woo
Published: September 2022

The biological reduction of slow degradation contaminants such as perchlorate (ClO4−) is considered to be a promising water treatment technology. The process is based on the ability of a specific mixed microbial culture to use perchlorate as an electron acceptor in the absence of oxygen. In this study, batch experiments were conducted to investigate the effect of nitrate on perchlorate reduction, the kinetic parameters of the Monod equation and the optimal ratio of acetate to perchlorate for the perchlorate reducing bacterial consortium. The results of this study suggest that acclimated microbial cultures can be applied to treat wastewater containing high concentrations of perchlorate. Reactor experiments were carried out with different hydraulic retention times (HRTs) to determine the optimal operating conditions. A fixed optimal HRT and the effect of nitrate on perchlorate reduction were investigated with various concentrations of the electron donor. The results showed that perchlorate reduction occurred after nitrate removal. Moreover, the presence of sulfate in wastewater had no effect on the perchlorate reduction. However, it had little effect on biomass concentration in the presence of nitrate during exposure to a mixed microbial culture, considering the nitrate as the inhibitor of perchlorate reduction by reducing the degradation rate. The batch scale experiment results illustrated that for efficient operation of perchlorate reduction, the optimal acetate to perchlorate ratio of 1.4:1.0 would be enough. Moreover, these experiments found the following results: the kinetic parameters equivalent to Y = 0.281 mg biomass/mg perchlorate, Ks = 37.619 mg/L and qmax = 0.042 mg perchlorate/mg biomass/h. In addition, anoxic–aerobic experimental reactor results verify the optimal HRT of 6 h for continuous application. Furthermore, it also illustrated that using 600 mg/L of acetate as a carbon source is responsible for 100% of nitrate reduction with less than 50% of the perchlorate reduction, whereas at 1000 mg/L acetate, approximately 100% reduction was recorded.
Green Synthesis of Endolichenic Fungi Functionalized Silver Nanoparticles: The Role in Antimicrobial, Anti-Cancer, and Mosquitocidal Activities
Authors: Mohanta Yugal Kishore , Nayak Debasis , Mishra Awdhesh Kumar , Chakrabartty Ishani , Ray Manjit Kumar , Mohanta Tapan Kumar , Tayung Kumananda , Rajaganesh Rajapandian , Vasanthakumaran Murugan , Muthupandian Saravanan , Murugan Kadarkarai , Sharma Gouridutta , Dahms Hans-Uwe , Hwang Jiang-Shiou
Published: September 2022

Green nanotechnology is currently a very crucial and indispensable technology for handling diverse problems regarding the living planet. The concoction of reactive oxygen species (ROS) and biologically synthesized silver nanoparticles (AgNPs) has opened new insights in cancer therapy. The current investigation caters to the concept of the involvement of a novel eco-friendly avenue to produce AgNPs employing the wild endolichenic fungus Talaromyces funiculosus. The synthesized Talaromyces funiculosus–AgNPs were evaluated with the aid of UV visible spectroscopy, dynamic light scattering (DLS), Fourier infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The synthesized Talaromyces funiculosus–AgNPs (TF-AgNPs) exhibited hemo-compatibility as evidenced by a hemolytic assay. Further, they were evaluated for their efficacy against foodborne pathogens Staphylococcus aureus, Streptococcus faecalis, Listeria innocua, and Micrococcus luteus and nosocomial Pseudomonas aeruginosa, Escherichia coli, Vibrio cholerae, and Bacillus subtilis bacterial strains. The synthesized TF-AgNPs displayed cytotoxicity in a dose-dependent manner against MDA-MB-231 breast carcinoma cells and eventually condensed the chromatin material observed through the Hoechst 33342 stain. Subsequent analysis using flow cytometry and fluorescence microscopy provided the inference of a possible role of intracellular ROS (OH−, O−, H2O2, and O2−) radicals in the destruction of mitochondria, DNA machinery, the nucleus, and overall damage of the cellular machinery of breast cancerous cells. The combined effect of predation by the cyclopoid copepod Mesocyclops aspericornis and TF-AgNPS for the larval management of dengue vectors were provided. A promising larval control was evident after the conjunction of both predatory organisms and bio-fabricated nanoparticles. Thus, this study provides a novel, cost-effective, extracellular approach of TF-AgNPs production with hemo-compatible, antioxidant, and antimicrobial efficacy against both human and foodborne pathogens with cytotoxicity (dose dependent) towards MDA-MB-231 breast carcinoma.
Adiponectin Intervention to Regulate Betatrophin Expression, Attenuate Insulin Resistance and Enhance Glucose Metabolism in Mice and Its Response to Exercise
Authors: Guo Qi , Cao Shicheng , Wang Xiaohong
Published: September 2022

Aims: Adiponectin stimulates mitochondrial biogenesis through peroxisome proliferator-activated receptor-coactivator1α (PGC-1α), a major regulator of mitochondrial biogenesis, and its effect on the genesis of insulin resistance is organ-specific. Expressed predominantly in fat and liver tissues, betatrophin is primarily involved in lipid metabolism, and could be a putative therapeutic target in metabolic syndrome and T2D. We hypothesized that the adiponectin pathway may regulate the production and/or secretion of betatrophin in liver. We aimed to determine whether exercise and adiponectin affect betatrophin to improve insulin resistance in mice. Methods: To investigate this hypothesis, we used wild-type C57BL/6 mice subjected to a high-fat diet, an exercise regimen, and i.p. injection of recombinant mouse adiponectin (Acrp30), and adiponectin knockout (Adipoq−/−) mice (C57BL/6 background) subjected to i.p. injection of Acrp30. Results: In Adipoq–/– mice, betatrophin levels in the plasma and liver were upregulated. In mice, plasma and liver betatrophin levels were significantly upregulated following a high-fat diet. Exercise and i.p. Acrp30 downregulated betatrophin levels and increased adiponectin mRNA and protein expression in the plasma and liver. The trend of change in PGC-1α and betatrophin levels in the liver was consistent. Conclusions/interpretation: Exercise reverses pathogenic changes in adiponectin and betatrophin levels in insulin-resistant mice. Exercise increased adiponectin levels and reduced betatrophin levels. Furthermore, exercise reduced betatrophin levels via adiponectin, which modulated the LKB1/AMPK/PGC-1α signaling axis but was not solely dependent on it for exerting its effects.
The Plastidial DIG5 Protein Affects Lateral Root Development by Regulating Flavonoid Biosynthesis and Auxin Transport in Arabidopsis
Authors: Liu Wei , Chen Tao , Liu Yajie , Le Quang Tri , Wang Ruigang , Lee Hojoung , Xiong Liming
Published: September 2022

To reveal the mechanisms underlying root adaptation to drought stress, we isolated and characterized an Arabidopsis mutant, dig5 (drought inhibition of lateral root growth 5), which exhibited increased sensitivity to the phytohormone abscisic acid (ABA) for the inhibition of lateral root growth. The dig5 mutant also had fewer lateral roots under normal conditions and the aerial parts were yellowish with a lower level of chlorophylls. The mutant seedlings also displayed phenotypes indicative of impaired auxin transport, such as abnormal root curling, leaf venation defects, absence of apical hook formation, and reduced hypocotyl elongation in darkness. Auxin transport assays with [3H]-labeled indole acetic acid (IAA) confirmed that dig5 roots were impaired in polar auxin transport. Map-based cloning and complementation assays indicated that the DIG5 locus encodes a chloroplast-localized tRNA adenosine deaminase arginine (TADA) that is involved in chloroplast protein translation. The levels of flavonoids, which are naturally occurring auxin transport inhibitors in plants, were significantly higher in dig5 roots than in the wild type roots. Further investigation showed that flavonoid biosynthetic genes were upregulated in dig5. Introduction of the flavonoid biosynthetic mutation transparent testa 4 (tt4) into dig5 restored the lateral root growth of dig5. Our study uncovers an important role of DIG5/TADA in retrogradely controlling flavonoid biosynthesis and lateral root development. We suggest that the DIG5-related signaling pathways, triggered likely by drought-induced chlorophyll breakdown and leaf senescence, may potentially help the plants to adapt to drought stress through optimizing the root system architecture.
The TGF-β/SMAD Signaling Pathway Prevents Follicular Atresia by Upregulating MORC2
Authors: Liu Jiying , Qi Nannan , Xing Wenwen , Li Mengxuan , Qian Yonghang , Luo Gang , Yu Shali
Published: September 2022

In mammals, female fertility is determined by the outcome of follicular development (ovulation or atresia). The TGF-β/SMAD signaling pathway is an important regulator of this outcome. However, the molecular mechanism by which the TGF-β/SMAD signaling pathway regulates porcine follicular atresia has not been fully elucidated. Microrchidia family CW-type zinc finger 2 (MORC2) is anovel epigenetic regulatory protein widely expressed in plants, nematodes, and mammals. Our previous studies showed that MORC2 is a potential downstream target gene of the TGF-β/SMAD signaling pathway. However, the role of MORC2 in porcine follicular atresia is unknown. To investigate this, qRT-PCR, western blotting, and TdT-mediated dUTP nick-end labeling were performed. Additionally, the luciferase activity assay was conductedto confirm that the TGF-β/SMAD signaling pathway regulates MORC2. Our results demonstrate that MORC2 is animportant anti-apoptotic molecule that prevents porcine follicular atresia via a pathway involving mitochondrial apoptosis, not DNA repair. Notably, this studyrevealsthat the TGF-β/SMAD signaling pathway inhibits porcine granulosa cell apoptosis by up-regulating MORC2. The transcription factor SMAD4 regulated the expression of MORC2 by binding to its promoter. Our results will help to reveal the mechanism underlying porcine follicular atresia and improve the reproductive efficiency of sows.
Parameters of Flow through Paravalvular Leak Channels from Computational Fluid Dynamics Simulations—Data from Real-Life Cases and Comparison with a Simplified Model
Authors: Kozłowski Michał , Wojtas Krzysztof , Orciuch Wojciech , Smolka Grzegorz , Wojakowski Wojciech , Makowski Łukasz
Published: September 2022

Background: Shear forces affecting erythrocytes in PVL channels can be calculated with computational fluid dynamics (CFD). The presence of PVLs is always associated with some degree of hemolysis in a simplified model of the left ventricle (LV); however, data from real-life examples is lacking. Methods: Blood flow through PVL channels was assessed in two variants. Firstly, a PVL channel, extracted from cardiac computed tomography (CCT), was placed in a simplified model of the LV. Secondly, a real-life model of the LV was created based on CCT data from a subject with a PVL. The following variables were assessed: wall shear stress (τw) shear stress in fluid (τ), volume of PVL channel with wall shear stress above 300 Pa (V300), duration of exposure of erythrocytes to shear stress above 300 Pa (Vt300) and compared with lactate dehydrogenase (LDH) activity levels. Results: τw and τ were higher in the simplified model. V300 and Vt300 were almost identical in both models. Conclusions: Parameters that describe blood flow through PVL channels can be reliably assessed in a simplified model. LDH levels in subjects with PVLs may be related to V300 and Vt300. Length and location of PVL channels may contribute to a risk of hemolysis in mitral PVLs.
The Use of Smart Devices for Mental Health Diagnosis and Care
Authors: Lautman Ziv , Lev-Ari Shahar
Published: September 2022

In 2019, more than 970 million people worldwide suffered from a mental disorder, with anxiety and depressive disorders as the leading culprits [...]
The Relationship between the Aqueous VEGF Level and the Severity of Type 1 Retinopathy of Prematurity
Authors: Liang Tianwei , Qian Zhuyun , Tao Yong , Peng Yaguang , Cui Yanhui , Zhang Chengyue , Peng Chunxia , Liu Lili , Hu Man , Li Li , Li Ningdong
Published: September 2022

Purpose: To analyze the relationship between the severity of type 1 retinopathy of prematurity (ROP) and the level of vascular endothelial growth factor (VEGF) in aqueous fluid. Methods: The aqueous VEGF levels of 49 patients (88 eyes) with type 1 ROP were retrospectively analyzed. These eyes were categorized into three groups according to the severity of disease: aggressive retinopathy of prematurity (A-ROP), threshold of ROP (T-ROP), and type 1 pre-threshold ROP (P-T-1). The differences in aqueous VEGF levels among these three groups were compared. The relationship between the aqueous VEGF level and the retinal changes of ROP, including the vessel tortuosity in zone I, and the location and stage of the ROP lesions, were also analyzed. Results: The aqueous VEGF level of the A-ROP group was the highest among the three groups, followed by those of the T-ROP and P-T-1 groups. The aqueous VEGF level was negatively correlated with the zone and the stage of the ROP diseases, while it was positively correlated with the venous tortuosity in zone I and had no relevance with the artery tortuosity in zone I. Conclusions: The aqueous VEGF level in A-ROP was the highest in type I ROP. The location of the ROP lesions and the venous tortuosity in zone I correlated with the aqueous VEGF level and could indicate the severity of ROP.
Dual Autoencoder Network with Separable Convolutional Layers for Denoising and Deblurring Images
Authors: Solovyeva Elena , Abdullah Ali
Published: September 2022

A dual autoencoder employing separable convolutional layers for image denoising and deblurring is represented. Combining two autoencoders is presented to gain higher accuracy and simultaneously reduce the complexity of neural network parameters by using separable convolutional layers. In the proposed structure of the dual autoencoder, the first autoencoder aims to denoise the image, while the second one aims to enhance the quality of the denoised image. The research includes Gaussian noise (Gaussian blur), Poisson noise, speckle noise, and random impulse noise. The advantages of the proposed neural network are the number reduction in the trainable parameters and the increase in the similarity between the denoised or deblurred image and the original one. The similarity is increased by decreasing the main square error and increasing the structural similarity index. The advantages of a dual autoencoder network with separable convolutional layers are demonstrated by a comparison of the proposed network with a convolutional autoencoder and dual convolutional autoencoder.
Development and Production of Artificial Test Swarf to Examine Wear Behavior of Running Engine Components—Geometrically Derived Designs
Authors: Brag Patrick , Piotter Volker , Plewa Klaus , Klein Alexander , Herzfeldt Mirko , Umbach Sascha
Published: September 2022

Subtractive manufacturing processes are usually accompanied by the occurrence of tiny flakes and swarf, which later on cause severe wear and damage, especially in moving components such as rolling or sliding bearings, pistons, etc. However, up until now, such detrimental effects have hardly been investigated. One reason is the lack of a definition of a typical design of debris particle. Therefore, the main goal of the project described in this paper was to elaborate a draft that defines standardized test particles. It had to be evaluated whether test particles could be adequately reproduced and whether they would reveal significant damage potential. Taking into account future mass fabrication, Micro Powder Injection Molding (MicroPIM) was chosen as a production method. Five different 3D designs of geometrically defined test particles were developed. The maximum size of each design was 1167 mm in green state; however, all samples shrank in size during sintering. Specially tailored feedstocks containing 42CrMo4 steel powders were used and the related molding, debinding and sintering procedures were developed. All particle geometries and related mold inserts were developed using a commercial software routine for the layout of runner systems, gate locations and ejector positions. The damage potential of the test particles was evaluated based on trials using journal bearing and shift valve test rigs. Although only a moderate degree of damage potential could be ascertained up until now, it can be expected that the artificial swarf will enable standardized wear test procedures to be developed.
The Effect of Intravenous Tranexamic Acid on Myomectomy: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Kathopoulis Nikolaos , Prodromidou Anastasia , Zacharakis Dimitrios , Chatzipapas Ioannis , Diakosavvas Michail , Kypriotis Konstantinos , Grigoriadis Themos , Protopapas Athanasios
Published: September 2022

Myomectomy is the preferred surgical treatment for symptomatic women with uterine myomas who wish to preserve their fertility. The procedure may be associated with significant intraoperative blood loss, which predisposes to increased transfusion rates and morbidity. The objective of our systematic review and meta-analysis is to investigate whether intravenous (IV) use of tranexamic acid (TXA) may reduce blood loss during myomectomy. Three electronic databases were screened until June 2022. The eligible studies were assessed for risk of bias. Four randomized controlled trials that reported outcomes from a total of 310 women were finally included in the meta-analysis—155 patients received intravenous TXA while the remaining 155 received placebo injection with normal saline or water for injection. Total estimated blood loss was significantly lower in patients who received TXA before myomectomy compared to control (230 patients MD −227.09 mL 95% CI −426.26, −27.91, p = 0.03). This difference in favor of TXA group remained when intraoperative and postoperative blood loss was separately analyzed. Postoperative hematocrit values and hemoglobin levels did not differ among the two groups (180 patients MD 0.67% 95% CI −0.26, 1.59, p = 0.16 and 250 patients MD 0.17 mg/dL 95% CI 0.07, 0.41, p = 0.17, respectively). The number of patients that received blood transfusion was also not different (310 patients OR 0.46 95% CI −0.14, 1.49, p = 0.19). Total operative time was significantly prolonged in control group compared to TXA (310 patients MD −16.39 min 95% CI −31.44, −1.34 p = 0.03). Our data show that the IV use of TXA may significantly reduce intraoperative blood loss in patients undergoing myomectomy and contribute to reduced operative time.
Swept-Source OCT Angiography Features in Patients after Macular Hole Surgery
Authors: Hwang Sunjin , Kang Min-Ho , Seong Mincheol , Cho Heeyoon , Shin Yong-Un
Published: September 2022

This study aimed to compare findings of optical coherence tomography (OCT) angiography (OCTA) between eyes with nearly recovered and partially recovered outer retina after full-thickness macular hole (FTMH) surgery and to identify OCTA findings associated with visual acuity. We retrospectively reviewed 30 patients who underwent surgery for idiopathic FTMH. Swept-source OCT (SS-OCT) and OCTA were performed preoperatively and at three and six months postoperatively. Subgroups were divided according to the integrity of the external limiting membranes and ellipsoid zones postoperatively. Correlations of best-corrected visual acuity six months postoperatively with SS-OCT and OCTA measurements were analyzed. There was no difference in preoperative retinal or choroidal vascular index on OCTA between the nearly and partially recovered groups. Six months postoperatively, the choriocapillaris flow-void area was significantly higher in the partially recovered group than in the fellow eye. The nearly recovered group showed better choroidal vascular flow, and vision at six months postoperatively correlated with the ratio of the operated eye’s choriocapillaris flow-void area to the fellow eye. Sufficient choriocapillaris flow on OCTA is associated with better outer retinal recovery and visual outcomes after macular hole surgery.
Plasma Angiotensin Converting Enzyme 2 (ACE2) Activity in Healthy Controls and Patients with Cardiovascular Risk Factors and/or Disease
Authors: Lim Hui Yin , Patel Sheila K. , Huang Ping , Tacey Mark , Choy Kay Weng , Wang Julie , Donnan Geoffrey , Nandurkar Harshal H. , Ho Prahlad , Burrell Louise M.
Published: September 2022

Angiotensin converting enzyme 2 (ACE2) is an endogenous negative regulator of the renin-angiotensin system, a key factor in the development of cardiovascular disease (CVD). ACE2 is also used by SARS-CoV-2 for host cell entry. Given that COVID-19 is associated with hypercoagulability, it is timely to explore the potential relationship between plasma ACE2 activity and the coagulation profile. In this cross-sectional study, ACE2 activity and global coagulation assays (GCA) including thromboelastography, thrombin, and fibrin generation were measured in adult healthy controls (n = 123; mean age 41 ± 17 years; 35% male) and in patients with cardiovascular risk factors and/or disease (n = 258; mean age 65 ± 14 years; 55% male). ACE2 activity was significantly lower in controls compared to patients with cardiovascular risk factors and/or disease (median 0.10 (0.02, 3.33) vs. 5.99 (1.95, 10.37) pmol/mL/min, p < 0.001). Of the healthy controls, 48% had undetectable ACE2 activity. Controls with detectable ACE2 had lower maximum amplitude (p < 0.001). In patients with cardiovascular risk factors and/or disease, those in the 3rd tertile were older and male (p = 0.002), with a higher Framingham grade and increased number of cardiovascular risk factors (p < 0.001). In conclusion, plasma ACE2 activity is undetectable to very low in young healthy controls with minimal clinically relevant associations to GCA. Patients with cardiovascular risk factors and/or disease have increased plasma ACE2 activity, suggesting that it may be an important biomarker of endothelial dysfunction and atherosclerosis.