bluuebunny/crossref_metadata_2025 huggingface dataset:
Transverse waves in nonlinearly elastic solids and the Milne—Pinney (or Ermakov) equation
Authors: Destrade Michel , Saccomandi Giuseppe
Published: February 2011

We establish a connection between the general equations of nonlinear elastodynamics and the nonlinear ordinary differential equation of Pinney [ Proc Amer Math Soc 1950; 1: 681]. As a starting point, we use the exact travelling wave solutions of nonlinear elasticity discovered by Carroll [ Acta Mechanica 1967; 3: 167]. The connection provides a method for finding new exact and approximate dynamic solutions for neo-Hookean and Mooney—Rivlin solids, and for the general third- and fourth-order elasticity models of incompressible solids.
Thermo-mechanical aspects of thermal flying-height control sliders for hard disk drives
Authors: Liu Nan , Zheng Jinglin , Bogy David B.
Published: February 2011

Thermal flying-height control sliders are currently used in most hard disk drives as an approach to increase the capacity and reliability. This paper discusses our recent numerical studies of the sliders’ flying performances, namely, the heat transfer between the slider and the disk, the flyability of the sliders at different altitudes, and the effect of the disk’s lubricant and roughness on the slider’s flying stability. We validate previous investigations based on a heat transfer model derived from the first-order slip theory and identify the instability regimes for a slider flying over a lubricated rough disk.
Assessing the instructional effectiveness of problem-based management education in Thailand: A longitudinal evaluation
Authors: Hallinger Philip , Jiafang Lu
Published: February 2011

Problem-based learning (PBL) has attracted increased interest in higher education due to claims that it provides a more active and productive learning environment.Yet, to date, most empirical research on the instructional effectiveness of PBL has been conducted in medical education. This article examines the instructional effectiveness of a problem-based curriculum at a business school in Thailand. The quasi-experimental study draws on seven years of student evaluation data to compare the instructional effectiveness of courses offered in a PBL track with other courses taught in the college. The results suggest that students perceived PBL as an effective approach to learning. PBL courses fostered a more active, engaging classroom environment that helped graduate management students understand how to apply theory to practice. The findings offer initial empirical support for the use of PBL in management education and counter the belief that Asian students are not responsive to learner centred approaches to education.
Teaching children with autism how to respond to the lures of strangers
Authors: Akmanoglu Nurgul , Tekin-Iftar Elif
Published: February 2011

A multiple probe design was used to assess the combined effects of videomodeling, graduated guidance and community-based instruction for teaching children with autism how to protect themselves from the lures of strangers. Parental opinions were also assessed in the study. Three children with autism (aged 6 to 11 years) participated in the study. Data indicated that videomodeling with graduated guidance and community-based instruction was effective for teaching children with autism how to protect themselves from the lures of strangers. Also it can be seen that participants maintained the acquired skill over time and applied it to novel settings. Furthermore, generalization was maintained over time. The parents of the participants reported positive opinions regarding the aims, procedures, and results of the study. Based on an evaluation of the findings, implications and future research needs are discussed.
Use of the Autism Diagnostic Observation Schedule (ADOS) in a clinical setting
Authors: Molloy Cynthia A. , Murray Donna S. , Akers Rachel , Mitchell Terry , Manning-Courtney Patricia
Published: February 2011

The aim of this study was to examine the Autism Diagnostic Observation Schedule (ADOS) as it is commonly used in clinical practice. ADOS classifications were compared to final diagnoses given by a multidisciplinary team to 584 children referred for evaluation for possible autism spectrum disorder (ASD) at the Cincinnati Children’s Hospital Medical Center. A total of 177 children were evaluated with a Module 1 (87 No Words), 198 with a Module 2 (90 < 5 years) and 209 with a Module 3. Of these, 142 (26%) were diagnosed with autism, 185 (32%) with non-autism ASD, and 257 (44%) with non-spectrum disorders. Sensitivities were moderate to high on both original and revised algorithms, while specificities were substantially lower than those previously reported. This difference is likely attributable to the composition of the sample that included many children with a broad array of developmental and behavioral disorders. The clinical impression of the team member who administered the ADOS was critical to the accuracy of the overall diagnosis. Using numeric scores alone resulted in misclassification from false positive results. The study highlights the importance of the qualitative interactions of the ADOS activities as well as the score in diagnostic decision making.
World history, civilizational analysis and historical sociology: Interpretations of non-Western civilizations in the work of Johann Arnason
Authors: Spohn Willfried
Published: February 2011

The aim of this article is to assess Arnason’s civilizational theory and methodology and their application to non-Western civilizations from a historical-comparative sociological perspective. Although civilizational analysis and historical sociology as historical-comparative orientations in sociology are closely connected, civilizational analysis concentrates particularly on the macro-history of civilizations, whereas historical-comparative sociology (particularly in its American variety) is orientated rather to a meso- and micro-analytical foundation of societal developments and therefore is more time- and context-sensitive. From such a perspective, the article reconstructs, first, Arnason’s theoretical and methodological approach to civilizational analysis and discusses his contribution to the civilizational origins and dynamics of the West as a measuring rod for non-European societies. Second, it then assesses Arnason’s two major exemplary civilizational studies: the Soviet model in Russia, Eastern Europe and the non-European world as well as the Japanese civilization in the broader East Asian civilizational context. The article concludes with a critical summary of Arnason’s highly innovative approach from the vantage point of a recently developing global orientation in historical and comparative sociology.
Eccentric modernity? An Islamic perspective on the civilizing process and the public sphere
Authors: Salvatore Armando
Published: February 2011

This article engages with Johann Arnason’s approach to the entanglements of culture and power in comparative civilizational analysis by simultaneously reframing the themes of the civilizing process and the public sphere. It comments and expands upon some key insights of Arnason concerning the work of Norbert Elias and Jürgen Habermas by adopting an ‘Islamic perspective’ on the processes of singularization of power from its cultural bases and of reconstruction of a modern collective identity merging the steering capacities and the participative ambitions of an emerging urban intelligentsia. The Islamic perspective provides insights into the interplay between civilizing processes and the modes through which cultural traditions innervate a modern public sphere. By revisiting key remarks of Arnason on Elias and Habermas, the Islamic perspective gains original contours, reflecting the search for a type of modernity that is eccentric to the mono-civilizational axis of the Western-led, global civilizing process. While this eccentric positioning entails a severe imbalance of power, it also relativizes the centrality of the modern state in the civilizing process and evidences some original traits of the public sphere in a non-Western context.
Infectious immune status in an obstetric population of Pakistani immigrants in Norway
Authors: Bjerke Soen Eng Yap , Vangen Siri , Holter Ellen , Stray-Pedersen Babill
Published: February 2011

Aim: To investigate cytomegalovirus, rubella, varicella, toxoplasma, and hepatitis B immune status and factors associated with susceptibility for infections among Pakistani pregnant women in Norway. Methods: A total of 206 pregnant Pakistani women living in Norway participated in the evaluation. Blood samples were collected during pregnancy and tested for IgG antibodies against cytomegalovirus, rubella virus, varicella-zoster virus, Toxoplasma gondii, and hepatitis B (HB) virus. Results: All women had IgG antibodies against cytomegalovirus. Positivity for rubella IgG was 92%, 93% had varicella IgG antibodies, while 17% had toxoplasma IgG. Eleven per cent were anti-HBc positive, one of whom was HBsAg positive, which means that blood and cervix secretions are infectious with risk of virus transmission to the baby at the time of birth. Six women were only anti-HBc positive, they may have low-level HB infection, and risk of transmission cannot be excluded. Age younger than 25 years, having less than two children, and having lived less than 5 years in Norway were factors significantly associated with varicella-seronegative status, and thus susceptible for primary infection. Conclusions: To decrease the incidence of neonatal and maternal morbidity related to rubella, varicella, toxoplasma, and hepatitis B in our Pakistani immigrant population, we should intensify our rubella antenatal screening programme and focus upon rubella vaccination postpartum. We should offer varicella-seronegative women immunisation, advise toxoplasma-seronegative women to avoid visit to their home country during pregnancy, and give hepatitis B vaccine to all newborns regardless of maternal HBsAg status.
Exploring social workers’ personal domains in rural practice
Authors: Wendt Sarah , Schiller Wendy , Cheers Brian , Francis Karen , Lonne Bob
Published: February 2011

• Summary: This article is grounded on Cheers, Darracott, and Lonne’s (2007) framework for conceptualizing the factors that influence rural social care practitioners in their work. Focusing on the ‘personal domain’ we report results of qualitative interviews with 22 social workers who were part of a larger sample of 82 Australian rural social practitioners. As part of the larger study, practitioners were asked how they defined social care, whether they practised it and if so how, factors influencing their practice, and the information bases they used.

• Findings: Analysis of the social work interviews identified that the personal domain had considerable influence on their day-to-day practice. Five themes emerged describing the personal domain: ‘life experiences’, ‘beliefs and values’, ‘ideas and theories’, ‘personal relationships’, and ‘personal characteristics’. We call for further research to conceptualize the domain more clearly, identify factors within it, and investigate how they influence practice, with special focus on how social workers’ personal moral-ethical frameworks influence their practice decisions.

• Application: Opening space to explore the personal domain challenges social work practitioners and students to critically reflect on how their life experiences, beliefs and values, ideas and theories and personal relationships and characteristics influence their practice. It also provides practitioners, employers, and professional organizations with knowledge they need to help social workers cope with the demands of practice.
The distributive justice of a global basic structure: A category mistake?
Authors: Follesdal Andreas
Published: February 2011

The present article explores ‘anti-cosmopolitan’ arguments that shared institutions above the state, such as there are, are not of a kind that support or give rise to distributive claims beyond securing minimum needs. The upshot is to rebut certain of these ‘anti-cosmopolitan’ arguments. Section 1 asks under which conditions institutions are subject to distributive justice norms. That is, which sound reasons support claims to a relative share of the benefits of institutions that exist and apply to individuals? Such norms may require strict equality, Rawls’ Difference Principle, or other constraints on inequality. Section 2 considers, and rejects, several arguments why existing international institutions are not thought to meet these conditions.
Allostatic Load and Child Maltreatment in Infancy
Authors: Katz Debra A. , Sprang Ginny , Cooke Circe
Published: February 2011

Allostatic load refers to the persistent dysregulation in physiologic systems that occurs in response to severe, frequent or chronic stressors. Childhood maltreatment has been associated with physical health problems, neurobiological changes and long-term emotional and behavioral difficulties. This article utilizes the framework of allostatic load to examine the impact of maltreatment in infancy through the case of an infant who experienced severe abuse and neglect by her biological mother and a traumatic separation from her foster mother. Children involved in the child welfare system are especially vulnerable to allostatic load because of disruptions in caretaking, frequent abuse or neglect and inadequate medical and mental health care. Allostatic load provides a way of conceptualizing the impact of severe maltreatment and disrupted attachment on neuroendocrine, cardiovascular and immune function and how these experiences may contribute to impaired physical health and premature death in very young children.
Chronic exertional compartment syndrome of the forearm: a case series of 12 patients treated with fasciotomy
Authors: Brown J. S. , Wheeler P. C. , Boyd K. T. , Barnes M. R. , Allen M. J.
Published: February 2011

Chronic exertional compartment syndrome of the forearm is rare in the published literature. We report the outcome of a series of 12 patients treated with fasciotomy over a 14 year period. All patients underwent dynamic intra-compartmental pressure testing using a slit catheter technique before surgery. Raised intra-compartmental pressures on exercise, typical symptoms and the absence of other diagnoses were criteria for offering surgical intervention. The superficial flexor, deep flexor and extensor compartments were released. Median follow-up was 9.5 years (range 7 months to 12 years). Median patient-reported percentage improvement after surgery was 88% (range 0%–100%). Median time to return to full activity was 9 weeks. Eleven out of 12 patients were satisfied, very satisfied or extremely satisfied with the outcome of surgery. Fasciotomy can be an effective treatment for chronic exertional compartment syndrome of the forearm.
Bevacizumab in metastatic breast cancer: when may it be used?
Authors: Goldfarb Shari B. , Hudis Clifford , Dickler Maura N.
Published: February 2011

Tumor angiogenesis, which is necessary for breast cancer growth, invasion and metastases, is regulated by pro-angiogenic factors such as vascular endothelial growth factor (VEGF). Bevacizumab is a recombinant humanized monoclonal antibody that targets VEGF. The addition of bevacizumab to chemotherapy has improved progression-free survival in the first- and second-line treatment of patients with advanced-stage breast cancer. In this article we review the clinical trials testing the utility of bevacizumab for the treatment of metastatic disease.
Sipuleucel-T in patients with metastatic castration-resistant prostate cancer: an insight for oncologists
Authors: Garcia Jorge A.
Published: February 2011

Sipuleucel-T represents a novel immunotherapeutic compound designed to stimulate an immune response against castration-resistant prostate cancer (CRPC). Sipuleucel-T is an autologous active cellular immunotherapy product, which includes autologous dendritic cells pulsed ex vivo with PAP2024, a recombinant fusion protein made of prostatic acid phosphatase and granulocyte-macrophage colony-stimulating factor. Despite the lack of prostate-specific antigen and objective response, a recent phase III randomized trial demonstrated a significant improvement in overall survival in asymptomatic and minimally symptomatic CRPC patients. This review summarizes the clinical development of Sipuleucel-T in CRPC that led to the regulatory approval of this compound in the USA.
Elevation of surfactant protein A in plasma and sputum in cigarette smokers
Authors: Mazur W. , Toljamo T. , Ohlmeier S. , Vuopala K. , Nieminen P. , Kobayashi H. , Kinnula V.L.
Published: February 2011

Serum surfactant protein (SP)-A has been postulated to associate with pulmonary fibrosis, but its role in cigarette smoking-related lung diseases is undefined.

SP-A levels in plasma and induced sputum in nonsmokers, smokers with respiratory symptoms (cough and/or phlegm) and symptom-free smokers were assessed using a validated EIA method. A total of 474 current smokers without any diseases or medications were enrolled and followed for 2 yrs with 111 of them succeeding in stopping.

Plasma SP-A level was detectable in all subjects and elevated in smokers independently of the symptoms compared to nonsmokers (p = 0.001). After 2 yrs of follow-up, the SP-A level was higher in those who continued smoking compared to the quitters (p<0.001). Plasma SP-A levels were associated with age, smoking history and lung function. Sputum (n = 109) SP-A was nondetectable in most nonsmokers, whereas smoking and symptoms increased sputum SP-A highly significantly (p = 0.001).

In conclusion, SP-A may be involved in pathogenesis of cigarette smoking-related lung diseases. Further studies are needed to elucidate the role of SP-A in chronic obstructive pulmonary disease.
Comparison of cardiac and pulmonary-specific quality-of-life measures in pulmonary arterial hypertension
Authors: Chen H. , De Marco T. , Kobashigawa E.A. , Katz P.P. , Chang V.W. , Blanc P.D.
Published: February 2011

Studies systematically comparing the performance of health-related quality-of-life (HRQoL) instruments in pulmonary arterial hypertension (PAH) are lacking. We sought to address this by comparing cardiac and respiratory-specific measures of HRQoL in PAH.

We prospectively assessed HRQoL in 128 patients with catheterisation-confirmed PAH at baseline and at 6, 12 and post-24 month follow-up visits. Cardiac-specific HRQoL was assessed using the Minnesota Living with Heart Failure Questionnaire (LHFQ); respiratory-specific HRQoL was assessed using the Airways Questionnaire 20 (AQ20); and general health status was assessed using the 36-item Short Form physical component summary (SF-36 PCS).

The LHFQ and AQ20 were highly intercorrelated. Both demonstrated strong internal consistency and converged with the SF-36 PCS. Both discriminated patients based on World Health Organization (WHO) functional class, 6-min walking distance (6MWD) and Borg dyspnoea index (BDI), with the exception of a potential floor effect associated with low 6MWD. The LHFQ was more responsive than the AQ20 to changes over time in WHO functional class, 6MWD and BDI. In multivariate analyses, the LHFQ and AQ20 were each longitudinal predictors of general health status, independent of functional class, 6MWD and BDI.

In conclusion, both cardiac-specific and respiratory-specific measures appropriately assess HRQoL in most patients with PAH. Overall, the LHFQ demonstrates stronger performance characteristics than the AQ20.
Mycobacterial hypersensitivity pneumonitis requires TLR9–MyD88 in lung CD11b+ CD11c+ cells
Authors: Daito H. , Kikuchi T. , Sakakibara T. , Gomi K. , Damayanti T. , Zaini J. , Tode N. , Kanehira M. , Koyama S. , Fujimura S. , Ebina M. , Ishii K.J. , Akira S. , Takai T. , Watanabe A. , Nukiwa T.
Published: February 2011

Mycobacteria are among the most common causes of hypersensitivity pneumonitis (HP), but controversy persists with regard to the involvement of the infectious potency of the organism in mycobacterial HP (hot tub lung). This study aimed to establish a mouse model of hot tub lung to clarify its pathophysiology.

Mice were exposed intranasally to formalin-killedMycobacterium aviumfrom a patient with hot tub lung (HP strain) or chronic pulmonary infection (non-HP strain), and bronchoalveolar lavage fluids and lung tissues were evaluated for allergic inflammation.

DeadM. aviumHP strain, but not non-HP strain, elicited marked HP-like pulmonary inflammation in wild-type mice. Although the inflammation was induced in mice lacking CD4 or CD8, the induction of HP-like responses was prevented in mice lacking myeloid differentiation factor (MyD)88 or Toll-like receptor (TLR)9. Cultured lung CD11c+ cells responded toM. aviumin a TLR9-dependent manner, and reconstitution of TLR9-/- mice with lung CD11c+ cells from wild-type mice restored the inflammatory responses. Further investigation revealed that pulmonary exposure toM. aviumHP strain increased the number of lung CD11b+ CD11c+ cells (dendritic cells) through TLR9 signalling.

Our results provide evidence that hot tub lung developsviathe mycobacterial engagement of TLR9–MyD88 signalling in lung CD11b+ dendritic cells independent of the mycobacterial infectious capacity.
The nephrogenic potential of the transcription factors osr1, osr2, hnf1b, lhx1 and pax8 assessed in Xenopus animal caps
Authors: Drews Christiane , Senkel Sabine , Ryffel Gerhart U
Published: February 2011

Background

The three distinct types of kidneys, pronephros, mesonephros and metanephros, develop consecutively in vertebrates. The earliest form of embryonic kidney, the pronephros, is derived from intermediate mesoderm and the first expressed genes localized in the pronephros anlage are the transcription factors osr1, osr2, hnf1b, lhx1 and pax8, here referred to as the early nephrogenic transcription factors. However, the pathway inducing nephrogenesis and the network of theses factors are poorly understood. Treatment of the undifferentiated animal pole explant (animal cap) of Xenopus with activin A and retinoic acid induces pronephros formation providing a powerful tool to analyze key molecular events in nephrogenesis.

Results

We have investigated the expression kinetics of the early nephrogenic transcription factors in activin A and retinoic acid treated animal caps and their potential to induce pronephric differentiation. In treated animal caps, expression of osr1, osr2, hnf1b and lhx1 are induced early, whereas pax8 expression occurs later implying an indirect activation. Activin A alone is able to induce osr2 and lhx1 after three hours treatment in animal caps while retinoic acid fails to induce any of these nephrogenic transcription factors. The early expression of the five transcription factors and their interference with pronephros development when overexpressed in embryos suggest that these factors potentially induce nephrogenesis upon expression in animal caps. But no pronephros development is achieved by either overexpression of OSR1, by HNF1B injection with activin A treatment, or the combined application of LHX1 and PAX8, although they influenced the expression of several early nephrogenic transcription factors in some cases. In an additional approach we could show that HNF1B induces several genes important in nephrogenesis and regulates lhx1 expression by an HNF1 binding site in the lhx1 promoter.

Conclusions

The early nephrogenic transcription factors play an important role in nephrogenesis, but have no pronephros induction potential upon overexpression in animal caps. They activate transcriptional cascades that partially reflect the gene activation initiated by activin A and retinoic acid. Significantly, HNF1B activates the lhx1 promoter directly, thus extending the known activin A regulation of the lhx1 gene via an activin A responsive element.
Sodium selectivity of Reissner's membrane epithelial cells
Authors: Yamazaki Muneharu , Kim Kyunghee X , Marcus Daniel C
Published: February 2011

Background

Sodium absorption by Reissner's membrane is thought to contribute to the homeostasis of the volume of cochlear endolymph. It was previously shown that the absorptive transepithelial current was blocked by amiloride and benzamil. The most commonly-observed target of these drugs is the epithelial sodium channel (ENaC), which is composed of the three subunits α-,β- and γ-ENaC. However, other less-selective cation channels have also been observed to be sensitive to benzamil and amiloride. The aim of this study was to determine whether Reissner's membrane epithelial cells could support parasensory K+ absorption via amiloride- and benzamil-sensitive electrogenic pathways.

Results

We determined the molecular and functional expression of candidate cation channels with gene array (GEO GSE6196), RT-PCR, and whole-cell patch clamp. Transcript expression analysis of Reissner's membrane detected no amiloride-sensitive acid-sensing ion channels (ASIC1a, ASIC2a, ASIC2b) nor amiloride-sensitive cyclic-nucleotide gated channels (CNGA1, CNGA2, CNGA4, CNGB3). By contrast, α-,β- and γ-ENaC were all previously reported as present in Reissner's membrane. The selectivity of the benzamil-sensitive cation currents was observed in whole-cell patch clamp recordings under Cl--free conditions where cations were the only permeant species. The currents were carried by Na+ but not K+, and the permeability of Li+ was greater than that of Na+ in Reissner's membrane. Complete replacement of bath Na+ with the inpermeable cation NMDG+ led to the same inward current as with benzamil in a Na+ bath.

Conclusions

These results are consistent with the amiloride/benzamil-sensitive absorptive flux of Reissner's membrane mediated by a highly Na+-selective channel that has several key characteristics in common with αβγ-ENaC. The amiloride-sensitive pathway therefore absorbs only Na+ in this epithelium and does not provide a parasensory K+ efflux route from scala media.
Selective patterning of ZnO nanorods on silicon substrates using nanoimprint lithography
Authors: Jung Mi-Hee , Lee Hyoyoung
Published: February 2011

In this research, nanoimprint lithography (NIL) was used for patterning crystalline zinc oxide (ZnO) nanorods on the silicon substrate. To fabricate nano-patterned ZnO nanorods, patterning of an n-octadecyltrichlorosilane (OTS) self-assembled monolayers (SAMs) on SiO2 substrate was prepared by the polymer mask using NI. The ZnO seed layer was selectively coated only on the hydrophilic SiO2 surface, not on the hydrophobic OTS SAMs surface. The substrate patterned with the ZnO seed layer was treated with the oxygen plasma to oxidize the silicon surface. It was found that the nucleation and initial growth of the crystalline ZnO were proceeded only on the ZnO seed layer, not on the silicon oxide surface. ZnO photoluminescence spectra showed that ZnO nanorods grown from the seed layer treated with plasma showed lower intensity than those untreated with plasma at 378 nm, but higher intensity at 605 nm. It is indicated that the seed layer treated with plasma produced ZnO nanorods that had a more oxygen vacancy than those grown from seed layer untreated with plasma. Since the oxygen vacancies on ZnO nanorods serve as strong binding sites for absorption of various organic and inorganic molecules. Consequently, a nano-patterning of the crystalline ZnO nanorods grown from the seed layer treated with plasma may give the versatile applications for the electronics devices.
Reported analgesic administration to rabbits undergoing experimental surgical procedures
Authors: Coulter Claire A , Flecknell Paul A , Leach Matthew C , Richardson Claire A
Published: February 2011

Background

It has become widely accepted that whenever animals are used in scientific procedures, the 3Rs principle of replacement, reduction and refinement described by William Russell and Rex Burch should be adhered to. Animals should be replaced with non-sentient alternatives if possible, the number of animals used should be reduced and experimental procedures should be refined to minimise pain, suffering and distress. Administration of analgesic agents to animals undergoing surgical procedures is a refinement used to alleviate pain. In this study, a structured literature review was carried out to examine current trends in analgesic administration to rabbits undergoing experimental surgical procedures.

Results

128 papers from 51 peer-reviewed journals were selected for inclusion in this review. Reporting administration of systemic analgesia to rabbits in peer-reviewed scientific papers increased significantly from 16% to 50% between 1995-1997 and 2005-2007 (P < 0.001). Papers that reported ethical approval were more likely than papers that did not specify approval to report systemic analgesic administration (P < 0.001). When systemic analgesics were administered, buprenorphine was the most frequently used agent and non-steroidal anti-inflammatory drugs were used less frequently than opioids in both time periods.

Conclusions

Although this review provides evidence that systemic analgesic administration to rabbits undergoing surgical procedures is increasing, rabbits do not always receive analgesia when they undergo experimental surgery. Other practices in rabbit perioperative care that could be improved, highlighted by this survey include: 1) changing the timing of analgesic administration by giving systemic analgesics pre- or perioperatively rather than only postoperatively, 2) using multimodal analgesia when pain is likely to be moderate to severe and 3) increasing the use of non-steroidal anti-inflammatory drugs and use of other techniques such as epidural analgesia particularly for orthopaedic procedures.
Combined subsegmentectomy: postoperative pulmonary function compared to multiple segmental resection
Authors: Yoshimoto Kentaro , Nomori Hiroaki , Mori Takeshi , Ohba Yasuomi , Shiraishi Kenji , Ikeda Koei
Published: February 2011

Background

For small peripheral c-T1N0M0 non-small cell lung cancers involving multiple segments, we have conducted a resection of subsegments belonging to different segments, i.e. combined subsegmentectomy (CSS), to avoid resection of multiple segments or lobectomy. Tumor size, location of tumor, and forced expiratory volume in 1 second (FEV1) of each preserved lobe were compared among the CSS, resection of single segment, and that of multiple segments.

Methods

FEV1 of each preserved lobe were examined in 17 patients who underwent CSS, 56 who underwent resection of single segment, and 41 who underwent resection of multiple segments, by measuring pulmonary function and lung-perfusion single-photon-emission computed tomography and computed tomography before and after surgery.

Results

Tumor size in the CSS was significantly smaller than that in the resection of multiple segments (1.4 ± 0.5 vs. 2.0 ± 0.8 cm, p = 0.002). Tumors in the CSS were located in the right upper lobe more frequently than those in the resection of multiple segments (53% vs. 5%, p < 0.001). Postoperative of FEV1 of each lobe after the CSS was higher than that after the resection of multiple segments (0.3 ± 0.2 vs. 0.2 ± 0.2 l, p = 0.07). Mean FEV1 of each preserved lobe per subsegment after CSS was significantly higher than that after resection of multiple segments (0.05 ± 0.03 vs. 0.03 ± 0.02 l, p = 0.02). There was no significant difference of these factors between the CSS and resection of single segment.

Conclusions

The CSS is effective for preserving pulmonary function of each lobe, especially for small sized lung cancer involving multiple segments in the right upper lobe, which has fewer segments than other lobes.
The reliability and validity of the Japanese version of the Levels of Emotional Awareness Scale (LEAS-J)
Authors: Igarashi Tetsuya , Komaki Gen , Lane Richard D , Moriguchi Yoshiya , Nishimura Hiroki , Arakawa Hiromi , Gondo Motoharu , Terasawa Yuri , Sullivan Corbet V , Maeda Motonari
Published: February 2011

Background

The Levels of Emotional Awareness Scale (LEAS) was developed to assess five levels of emotional awareness: bodily sensations, action tendencies, single emotions, blends of emotion, and combinations of blends. It is a paper and pencil performance questionnaire that presents 20 emotion-evoking scenes. We developed a Japanese version of the LEAS (LEAS-J), and its reliability and validity were examined.

Methods

The LEAS-J level was independently assessed by two researchers who scored each response according to the LEAS scoring manual. High inter-rater reliability and internal consistency were obtained for the LEAS-J. Measures were socioeconomic status, LEAS-J, Toronto Alexithymia Scale-20 (TAS-20), Interpersonal Reactivity Index (IRI), and NEO Five-Factor Inventory (NEO-FFI). TAS-20, IRI and NEO-FFI were the measures used to explore the construct validity of LEAS-J, as it was predicted that higher scores on the LEAS-J would be related to fewer alexithymic features, greater empathetic ability, and a greater sense of cooperation with others. Questionnaires were completed by 344 university students.

Results

The criterion-referenced validity was determined: a significant negative relationship was found with the externally-oriented thinking scores of TAS-20, and positive relationships were found with fantasy, perspective taking, and empathic concern on IRI and with extraversion, openness to experience, and agreeableness on NEO-FFI.

Conclusions

Consistent with our expectations, the findings provide evidence that the LEAS-J has good reliability and validity. In addition, women had significantly higher scores than men on LEAS-J, showing that the gender difference identified in the original LEAS was cross-culturally consistent.
A simple work flow for biologically inspired model reduction - application to early JAK-STAT signaling
Authors: Quaiser Tom , Dittrich Anna , Schaper Fred , Mönnigmann Martin
Published: February 2011

Background

Modeling of biological pathways is a key issue in systems biology. When constructing a model, it is tempting to incorporate all known interactions of pathway species, which results in models with a large number of unknown parameters. Fortunately, unknown parameters need not necessarily be measured directly, but some parameter values can be estimated indirectly by fitting the model to experimental data. However, parameter fitting, or, more precisely, maximum likelihood parameter estimation, only provides valid results, if the complexity of the model is in balance with the amount and quality of the experimental data. If this is the case the model is said to be identifiable for the given data. If a model turns out to be unidentifiable, two steps can be taken. Either additional experiments need to be conducted, or the model has to be simplified.

Results

We propose a systematic procedure for model simplification, which consists of the following steps: estimate the parameters of the model, create an identifiability ranking for the estimated parameters, and simplify the model based on the identifiability analysis results. These steps need to be applied iteratively until the resulting model is identifiable, or equivalently, until parameter variances are small. We choose parameter variances as stopping criterion, since they are concise and easy to interpret. For both, the parameter estimation and the calculation of parameter variances, multi-start parameter estimations are run on a parallel cluster. In contrast to related work in systems biology, we do not suggest simplifying a model by fixing some of its parameters, but change the structure of the model.

Conclusions

We apply the proposed approach to a model of early signaling events in the JAK-STAT pathway. The resulting model is not only identifiable with small parameter variances, but also shows the best trade-off between goodness of fit and model complexity.
Contribution of large genomic BRCA1 alterations to early-onset breast cancer selected for family history and tumour morphology: a report from The Breast Cancer Family Registry
Authors: Smith Letitia D , Tesoriero Andrea A , Wong Ee M , Ramus Susan J , O'Malley Frances P , Mulligan Anna Marie , Terry Mary Beth , Senie Ruby T , Santella Regina M , John Esther M , Andrulis Irene L , Ozcelik Hilmi , Daly Mary B , Godwin Andrew K , Buys Saundra S , Fox Stephen , Goldgar David E , Giles Graham G , Hopper John L , Southey Melissa C
Published: February 2011

Introduction

Selecting women affected with breast cancer who are most likely to carry a germline mutation in BRCA1 and applying the most appropriate test methodology remains challenging for cancer genetics services. We sought to test the value of selecting women for BRCA1 mutation testing on the basis of family history and/or breast tumour morphology criteria as well as the value of testing for large genomic alterations in BRCA1 .

Methods

We studied women participating in the Breast Cancer Family Registry (BCFR), recruited via population-based sampling, who had been diagnosed with breast cancer before the age of 40 years who had a strong family history of breast or ovarian cancer (n = 187) and/or a first primary breast tumour with morphological features consistent with carrying a BRCA1 germline mutation (n = 133; 37 met both criteria). An additional 184 women diagnosed before the age of 40 years who had a strong family history of breast or ovarian cancer and who were not known to carry a germline BRCA1 mutation were selected from among women who had been recruited into the BCFR from clinical genetics services. These 467 women had been screened for BRCA1 germline mutations, and we expanded this testing to include a screen for large genomic BRCA1 alterations using Multiplex Ligation-dependent Probe Amplification.

Results

Twelve large genomic BRCA1 alterations were identified, including 10 (4%) of the 283 women selected from among the population-based sample. In total, 18 (12%), 18 (19%) and 16 (43%) BRCA1 mutations were identified in the population-based groups selected on the basis of family history only (n = 150), the group selected on the basis of tumour morphology only (n = 96) and meeting both criteria (n = 37), respectively.

Conclusions

Large genomic alterations accounted for 19% of all BRCA1 mutations identified. This study emphasises the value of combining information about family history, age at diagnosis and tumour morphology when selecting women for germline BRCA1 mutation testing as well as including a screen for large genomic alterations.
Manual muscle strength testing of critically ill patients: feasibility and interobserver agreement
Authors: Hough Catherine L , Lieu Binh K , Caldwell Ellen S
Published: February 2011

Introduction

It has been proposed that intensive care unit (ICU)-acquired weakness (ICUAW) should be assessed using the sum of manual muscle strength test scores in 12 muscle groups (the sum score). This approach has been tested in patients with Guillain-Barré syndrome, yet little is known about the feasibility or test characteristics in other critically ill patients. We studied the feasibility and interobserver agreement of this sum score in a mixed cohort of critically ill and injured patients.

Methods

We enrolled patients requiring more than 3 days of mechanical ventilation. Two observers performed systematic strength assessments of each patient. The primary outcome measure was interobserver agreement of weakness as a binary outcome (ICUAW is sum score less than 48; "no ICUAW" is a sum score greater than or equal to 48) using the Cohen's kappa statistic.

Results

We identified 135 patients who met the inclusion criteria. Most were precluded from study participation by altered mental status or polytrauma. Thirty-four participants were enrolled, and 30 of these individuals completed assessments conducted by both observers. Six met the criteria for ICUAW recorded by at least one observer. The observers agreed on the diagnosis of ICUAW for 93% of participants (Cohen's kappa = 0.76; 95% confidence interval (CI), 0.44 to 1.0). Observer agreement was fair in the ICU (Cohen's kappa = 0.38), and agreement was perfect after ICU discharge (Cohen's kappa = 1.0). Absolute values of sum scores were similar between observers (intraclass correlation coefficient 0.83; 95% CI, 0.67 to 0.91), but they differed between observers by six points or more for 23% of the participants.

Conclusions

Manual muscle testing (MMT) during critical illness was not possible for most patients because of coma, delirium and/or injury. Among patients who were able to participate in testing, we found that interobserver agreement regarding ICUAW was good, particularly when evaluated after ICU discharge. MMT is insufficient for early detection of ICU-acquired neuromuscular dysfunction in most patients and may be unreliable during critical illness.
Prognostic value of circulating amino-terminal pro-C-type natriuretic peptide in critically ill patients
Authors: Koch Alexander , Voigt Sebastian , Sanson Edouard , Dückers Hanna , Horn Andreas , Zimmermann Henning W , Trautwein Christian , Tacke Frank
Published: February 2011

Introduction

C-type natriuretic peptide (CNP) is a paracrine molecule which is mainly synthesized in the vasculature. High levels have been reported in sepsis, and CNP has been proposed as a biomarker predicting sepsis in traumatized patients. We aimed at evaluating the diagnostic and prognostic value of N-terminal pro-CNP (NT-proCNP) for predicting sepsis, disease severity and mortality in critically ill medical patients.

Methods

273 critically ill patients (197 patients with sepsis or septic shock, 76 without evidence of sepsis) and 43 healthy controls were consecutively included in a prospective clinical single-center non-interventional study at the Medical Intensive Care Unit, RWTH-University Aachen, Germany. Patients' outcome was followed for about 1 year. NT-proCNP serum concentrations were determined upon ICU admission, as well as in the mornings of day 3 and day 7 after admission. Intensive care treatment measures as well as routine and experimental laboratory parameters were recorded and analyzed.

Results

NT-proCNP serum concentrations upon admission to the ICU were elevated in critically ill patients as compared with healthy controls. Patients with sepsis had significantly higher NT-proCNP levels than non-sepsis patients. NT-proCNP was strongly associated with inflammatory parameters (i.e. C-reactive protein, procalcitonin and TNF-α), biomarkers of organ dysfunction and clinical composite scores (APACHE-II, SOFA, SAPS2). NT-proCNP levels at admission and day 3 were found to be a strong predictive marker for ICU- and overall survival. Moreover, a decline of serum NT-proCNP after admission to the ICU was associated with reduced mortality. The predictive power of serum NT-proCNP was similar to 'conventional' prognostic tools such as clinical scores.

Conclusions

NT-proCNP is significantly elevated in critically ill patients, with highest levels in sepsis. Inflammation as well as organ function are strongly associated with NT-proCNP serum concentrations. Low initial NT-proCNP levels and a decline during initial treatment indicate a favourable ICU- and long-term outcome.
A simple functional marker to predict the need for prolonged mechanical ventilation in patients with Guillain-Barré syndrome
Authors: Fourrier François , Robriquet Laurent , Hurtevent Jean-François , Spagnolo Shirley
Published: February 2011

Introduction

Patients suffering from Guillain-Barré syndrome (GBS) may frequently develop an acute respiratory failure and need ventilatory support. Immune therapy using plasma exchange or immunoglobulins has modified the natural course of the disease and by decreasing the length of the plateau phase, may induce a rapid improvement in ventilatory function. However a substantial proportion of patients still require prolonged mechanical ventilation (MV) and tracheotomy. The present study was designed to search for simple functional markers that could predict the need for prolonged MV just after completion of immune therapy.

Methods

We analyzed the data collected in a cohort of patients with GBS admitted to the intensive care unit (ICU) of our university hospital between 1996 and 2009. Demographic, clinical, biological and electrophysiologic data, results of sequential spirometry, and times of endotracheal intubation, tracheotomy, and MV weaning were prospectively collected for all patients. Sequential daily neurological testing used standardized data collection by the same investigators all along the study period. Results were compared by single and multiple regression analysis at admission to ICU and at the end of immune therapy, according to the need and duration of MV (≤ or > 15 days).

Results

Sixty-one patients with severe GBS were studied. Sixty-six percent required MV (median length: 24 days). The lack of foot flexion ability at ICU admission and at the end of immunotherapy was significantly associated with MV length > 15 days (positive predictive value: 82%; odds ratio: 5.4 [1.2 - 23.8] and 82%; 6.4 [1.4 - 28.8], respectively). The association of a sciatic nerve motor conduction block with the lack of foot flexion at the end of immunotherapy was associated with prolonged MV with a 100% positive predictive value.

Conclusions

In patients admitted to ICU with Guillain-Barré syndrome and acute respiratory failure, the lack of foot flexion ability at the end of immune therapy predicts a prolonged duration of MV. Combined with a sciatic motor conduction block, it may be a strong argument to perform an early tracheotomy.
Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
Authors: Aird Daniel , Ross Michael G , Chen Wei-Sheng , Danielsson Maxwell , Fennell Timothy , Russ Carsten , Jaffe David B , Nusbaum Chad , Gnirke Andreas
Published: February 2011

Despite the ever-increasing output of Illumina sequencing data, loci with extreme base compositions are often under-represented or absent. To evaluate sources of base-composition bias, we traced genomic sequences ranging from 6% to 90% GC through the process by quantitative PCR. We identified PCR during library preparation as a principal source of bias and optimized the conditions. Our improved protocol significantly reduces amplification bias and minimizes the previously severe effects of PCR instrument and temperature ramp rate.
Embryonic origin of the adult hematopoietic system: advances and questions
Authors: Medvinsky Alexander , Rybtsov Stanislav , Taoudi Samir
Published: February 2011

Definitive hematopoietic stem cells (HSCs) lie at the foundation of the adult hematopoietic system and provide an organism throughout its life with all blood cell types. Several tissues demonstrate hematopoietic activity at early stages of embryonic development, but which tissue is the primary source of these important cells and what are the early embryonic ancestors of definitive HSCs? Here, we review recent advances in the field of HSC research that have shed light on such questions, while setting them into a historical context, and discuss key issues currently circulating in this field.
Neuronal remodeling and apoptosis require VCP-dependent degradation of the apoptosis inhibitor DIAP1
Authors: Rumpf Sebastian , Lee Sung Bae , Jan Lily Yeh , Jan Yuh Nung
Published: February 2011

The regulated degeneration of axons or dendrites (pruning) and neuronal apoptosis are widely used during development to determine the specificity of neuronal connections. Pruning and apoptosis often share similar mechanisms; for example, developmental dendrite pruning of Drosophila class IV dendritic arborization (da) neurons is induced by local caspase activation triggered by ubiquitin-mediated degradation of the caspase inhibitor DIAP1. Here, we examined the function of Valosin-containing protein (VCP), a ubiquitin-selective AAA chaperone involved in endoplasmic reticulum-associated degradation, autophagy and neurodegenerative disease, in Drosophila da neurons. Strong VCP inhibition is cell lethal, but milder inhibition interferes with dendrite pruning and developmental apoptosis. These defects are associated with impaired caspase activation and high DIAP1 levels. In cultured cells, VCP binds to DIAP1 in a ubiquitin- and BIR domain-dependent manner and facilitates its degradation. Our results establish a new link between ubiquitin, dendrite pruning and the apoptosis machinery.