bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing potency and binding kinetics of soluble adenylyl cyclase (sAC) inhibitors to maximize therapeutic potential
Authors: Rossetti Thomas , Ferreira Jacob , Ghanem Lubna , Buck Hannes , Steegborn Clemens , Myers Robert W. , Meinke Peter T. , Levin Lonny R. , Buck Jochen
Published: September 2022

In mammalian cells, 10 different adenylyl cyclases produce the ubiquitous second messenger, cyclic adenosine monophosphate (cAMP). Amongst these cAMP-generating enzymes, bicarbonate (HCO3−)-regulated soluble adenylyl cyclase (sAC; ADCY10) is uniquely essential in sperm for reproduction. For this reason, sAC has been proposed as a potential therapeutic target for non-hormonal contraceptives for men. Here, we describe key sAC-focusedin vitroassays to identify and characterize sAC inhibitors for therapeutic use. The affinity and binding kinetics of an inhibitor can greatly influencein vivoefficacy, therefore, we developed improved assays for assessing these efficacy defining features.
Genome-wide identification and analysis of anthocyanin synthesis-related R2R3-MYB genes in Cymbidium goeringii
Authors: Chen Jiating , Bi Yuan-Yang , Wang Qian-Qian , Liu Ding-Kun , Zhang Diyang , Ding Xiangqing , Liu Zhong-Jian , Chen Shi-Pin
Published: September 2022

The MYB gene family plays a vital regulatory role in plant metabolism, stress response, and floral color. The R2R3-MYB gene family of C. goeringii was identified, and its expression was analyzed using bioinformatics in this article. The R2R3-MYB genes of Arabidopsis thaliana were used as a reference to determine 104 CgMYB genes and categorize them into 22 subfamilies. Exon/intron organizations and conserved motif analysis revealed that the majority of CgMYB genes were highly conserved, and chromosome localization and collinearity analysis provided evidence of tandem duplication and segmental duplication events, indicating the phenomenon of gene family expansion and contraction. The function of CgMYB genes was analyzed by cis-acting element and gene ontology (GO) enrichment. In addition, we selected CgMYB91 and CgMYB32 for RT–qPCR, suggesting that CgMYB91 and CgMYB32 are associated with anthocyanin formation. In short, this study provides a comprehensive and specific function of the R2R3-MYB transcription factors (TFs) in orchids.
A recombinant subunit vaccine candidate produced in plants elicits neutralizing antibodies against SARS-CoV-2 variants in macaques
Authors: Khorattanakulchai Narach , Srisutthisamphan Kanjana , Shanmugaraj Balamurugan , Manopwisedjaroen Suwimon , Rattanapisit Kaewta , Panapitakkul Chalisa , Kemthong Taratorn , Suttisan Nutchanat , Malaivijitnond Suchinda , Thitithanyanont Arunee , Jongkaewwattana Anan , Phoolcharoen Waranyoo
Published: September 2022

Since the outbreak of the coronavirus disease (COVID) pandemic in 2019, the development of effective vaccines to combat the infection has been accelerated. With the recent emergence of highly transmissible severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOC), there are concerns regarding the immune escape from vaccine-induced immunity. Hence an effective vaccine against VOC with a potent immune response is required. Our previous study confirmed that the two doses of the plant-produced receptor-binding domain (RBD) of SARS-CoV-2 fused with the Fc region of human IgG1, namely Baiya SARS-CoV-2 Vax 1, showed high immunogenicity in mice and monkeys. Here, we aimed to evaluate the immunogenicity of a three-dose intramuscular injection of Baiya SARS-CoV-2 Vax 1 on days 0, 21, and 133 in cynomolgus monkeys. At 14 days after immunization, blood samples were collected to determine RBD-specific antibody titer, neutralizing antibody, and pseudovirus neutralizing antibody titers. Immunized monkeys developed significantly high levels of antigen-specific antibodies against SARS-CoV-2 compared to the control group. Interestingly, the sera collected from immunized monkeys also showed a neutralizing antibody response against the SARS-CoV-2 VOCs; Alpha, Beta, Gamma, Delta, and Omicron. These findings demonstrate that a three-dose regimen of Baiya SARS-CoV-2 Vax 1 vaccine elicits neutralizing immune response against SARS-CoV-2 variants.
Optimal exogenous calcium alleviates the damage of Snow-melting agent to Salix matsudana seedlings
Authors: Li Hui , Huang Shenglan , Ren Chengshuai , Weng Xiaohang , Zhang Songzhu , Liu Liying , Pei Jiubo
Published: September 2022

As the main component of snowmelt agents, NaCl is widely used in northern winters and significantly impacts the expected growth of garden plants in north China. Salix matsudana is also faced with salt stress caused by snowmelt, which seriously affects its development as the main tree species in the northern landscape. However, how exogenous calcium alleviates salt stress in Salix matsudana is not yet clear. In this study, the indicators of growth indices, photosynthetic characteristics and stress resistance were measured by hydroponic assays in combination with three NaCl conditions (0, 50 and 200 mmol·L-1) and five calcium concentrations (0, 2.5, 5, 10 and 20 mmol·L-1). The study’s results indicated that the application of exogenous calcium remarkably promoted the growth of Salix matsudana seedlings under NaCl stress. When the exogenous calcium concentration was 10 mmol·L-1, the plant height and basal diameter of Salix matsudana seedlings increased significantly, and the biomass of all parts reached the maximum (P< 0.05). Exogenous calcium can substantially improve the photosynthesis of Salix matsudana seedlings under salt stress. The photosynthetic parameters, photosynthetic pigment content and photosynthetic product synthesis of Salix matsudana seedlings were significantly increased at an exogenous calcium concentration of 10 mmol·L-1, and the photosynthetic level of Salix matsudana seedlings reached the highest value. The chlorophyll fluorescence parameters (Fv/Fm, Fv/F0) of Salix matsudana seedlings were significantly decreased under different concentrations of NaCl stress. The maximum photochemical efficiency (Fv/Fm) and potential photochemical efficiency (Fv/F0) of Salix matsudana seedlings peaked when the exogenous calcium concentration was 10 mmol·L-1, which was significantly higher than that of the other treatments (P< 0.05). The water use efficiency of Salix matsudana was affected considerably by NaCl stress. The WUE and iWUE peak values of Salix matsudana were significantly higher than those of other calcium concentrations at 10 mmol·L-1 (P< 0.05). Exogenous calcium can increase the activities of CAT, SOD and POD enzymes in Salix matsudana seedlings under different NaCl concentrations. Under NaCl stress, adding exogenous calcium promoted the survival rate and growth of Salix matsudana seedlings. In conclusion, the optimum exogenous calcium concentration for Salix matsudana seedlings was 10 mmol·L-1. High or low concentrations of exogenous calcium did not achieve the best results in alleviating salt stress in Salix matsudana.
Online users’ donation behavior to medical crowdfunding projects: Mediating analysis of social presence and perceived differences in trust
Authors: Zhang Tao , Zhang Qianyu , Jiang Rong , Gao Tilei , Yang Ming
Published: September 2022

Perceived trust is a key factor affecting the behavior to donate online. In order to further explore the factors and influencing mechanisms that affect the success of medical crowdfunding projects, this paper, combined with the Stimulus-Organism-Response (S-O-R) theory, introduces the mediating variable of social presence and perceived differences in trust, and constructs a model of online users’ donation behavior to medical crowdfunding projects. We collected 437 valid samples through a questionnaire survey, and processed the data with SPSS and Amos software to test and analyze the theoretical model. The research results showed that project description and user participation have a significant positive effect on social presence; project transparency and patient identity have a significant negative effect on perceived differences in trust; social presence has a positive effect on donation behavior, while the perceived difference in trust has a negative effect; social presence and perceived differences in trust play a mediating role respectively; there is no significant effect of patient status on social presence. This study further expands the application of social presence and perceived differences in trust in medical crowdfunding, and provides a theoretical basis for the success of medical crowdfunding projects.
Unsaid thoughts: Thinking in the absence of verbal logical connectives
Authors: Lobina David J. , Demestre Josep , García-Albea José E. , Guasch Marc
Published: September 2022

Combining two thoughts into a compound mental representation is a central feature of our verbal and non-verbal logical abilities. We here approach this issue by focusing on the contingency that while natural languages have typically lexicalised only two of the possible 16 binary connectives from formal logic to express compound thoughts—namely, the coordinatorsandandor—some of the remainder appear to be entertainable in a non-verbal, conceptual representational system—alanguage of thought—and this suggests a theoretical split between the “lexicalisation” of the connectives and the “learnability” of invented words corresponding to unlexicalised connectives. In avisual worldexperiment aimed at tracking comprehension-related as well as reasoning-related aspects of the capacity to represent compound thoughts, we found that participants are capable of learning and interpreting a made-up word standing for logic's NAND operator, a result that indicates that unlexicalised logical connectives are not only conceptually available, but can also be mapped onto new function words, as in the case of coordinators, or connectives, a class of words that do not usually admit new coinages.
Centers of value and the quest for meaning in faith development: A measurement approach
Authors: Mallery Suzanne T. , Mallery Paul
Published: September 2022

James Fowler’s model of faith development conceptualized “faith” as the quest for and maintenance of meaning oriented around centers of value which may or may not be religious or spiritual in nature. Although this model foreshadowed later work in meaning in life, substantial bodies of literature have developed in each area, almost entirely independently of the other. Integration has been hindered by measurement difficulties in faith development work. Fowler’s stages of faith development and their reformulation as Streib’s religious styles are usually measured through either a lengthy Faith Development Interview or short measures that do not assess the breadth of domains covered in the interview. These short measures are in many cases oriented around religious faith and impossible for a non-believer to answer. Embedded within the original model and the interview are aspects of faith development including perspective taking, social horizon, morality, locus of authority, form of world coherence, and symbolic function. A new Centers of Value and Quest for Meaning Scale is proposed to assess the aspects, allow non-believers to respond, tap centers of value that are not religious, and eventually address the theoretical assumption of structural wholeness across aspects. In a series of exploratory factor analyses, factors for each adult stage/style emerged for most of the aspects. This supports the potential importance of assessing the aspects and allows for more than one methodology to assess them.
What are the essential components to implement individual-focused interventions for well-being and burnout in critical care healthcare professionals? A realist expert opinion
Authors: Adnan Nurul B. B. , Baldwin Claire , Dafny Hila A. , Chamberlain Diane
Published: September 2022

Background

This study aimed to determine what, how, and under what circumstances individual-focused interventions improve well-being and decrease burnout for critical care healthcare professionals.

Method

This realist approach, expert opinion interview, was guided by the Realist And Meta-narrative Evidence Synthesis: Evolving Standards II (RAMESES II) guidelines. Semi-structured interviews with critical care experts were conducted to ascertain current and nuanced information on a set of pre-defined individual interventions summarized from a previous umbrella review. The data were appraised, and relationships between context, mechanisms, and outcomes were extracted, which created theory prepositions that refined the initial program theory.

Results

A total of 21 critical care experts were individually interviewed. By understanding the complex interplay between organizational and personal factors that influenced intervention uptake, it was possible to decipher the most likely implementable intervention for critical care healthcare professionals. The expert recommendation suggested that interventions should be evidence-based, accessible, inclusive, and collaborative, and promote knowledge and skill development. Unique mechanisms were also required to achieve the positive effects of the intervention due to the presence of contextual factors within critical care settings. Mechanisms identified in this study included the facilitation of self-awareness, self-regulation, autonomy, collaboration, acceptance, and inclusion (to enable a larger reach to different social groups).

Conclusion

This validation of a theoretical understanding of intervention that addressed well-being and burnout in critical care healthcare professionals by expert opinion demonstrated essential mechanisms and contextual factors to consider when designing and implementing interventions. Future research would benefit by piloting individual interventions and integrating these new theoretical findings to understand better their effectiveness for future translation into the “real-world” setting.
Factors that determine mental health professionals’ decision to support home-based video consultations – A qualitative study
Authors: Moeller Anne Marie , Hansen Jens Peter , Andersen Pernille Tanggaard
Published: September 2022

Introduction

Using videoconferencing for consulting with patients in the mental health services has been shown in interventions to be similarly effective as when meeting in person. In practice, it often makes more sense to use video consultations with patients in a more flexible way than interventions permit. The aim of this study was to investigate what constitutes a professional video consultation from the perspectives of mental health professionals and explore what is of importance for the establishment and realization of video consultations in practice.

Materials and methods

A Grounded Theory methodology approach based on Corbin and Strauss was used. Data collection consisted of participant observations of introductory events followed by individual interviews with mental health professionals who had used video consultations with patients.

Findings

Mental health professionals believed that a professional video consultation was one that was not inferior to an in-person consultation but offered something else, such as more and easier access, accommodating patients’ needs and wishes. At the same time, it should not interfere with the treatment quality, e.g., by hampering communication and therapeutic tasks. The expected treatment quality was based on an individual assessment of the patient and varied from clinician to clinician. The implementation process and support which the organization provided affected the clinicians’ attitudes as well as the clinicians’ experiences and hence how the clinicians assessed the quality of the service.

Conclusion

Perceived usefulness, patient demands, and close IT support will positively impact the establishment and realization of video consultations whereas high workload and technical problems would hamper it.
The impact of the COVID-19 pandemic on job satisfaction among professionally active nurses in five European countries
Authors: Makowicz Dawid , Lisowicz Katarzyna , Bryniarski Krzysztof , Dziubaszewska Renata , Makowicz Natalia , Dobrowolska Beata
Published: September 2022

The COVID-19 pandemic has negatively affected the work of many medical professionals, including the group of nurses. This study aimed at assessing the impact of the COVID-19 pandemic on job satisfaction of nursing staff in five European countries. The study was conducted using the Job Satisfaction Scale (SSP) and original questions on the job satisfaction. The cross-sectional online study was conducted with a sample of 1,012 professionally active nurses working in Poland, Germany, Italy, Great Britain and Sweden, who assessed their job satisfaction before (retrospectively) and during the pandemic. The results showed a significant decrease in job satisfaction due to the need to perform it during the pandemic caused by the SARS-CoV-2 virus. In 8 out of 10 examined parameters of job satisfaction, a statistically significant decrease in job satisfaction was observed at the level of p < 0.05. Among the examined factors influencing job satisfaction, the highest decrease was recorded based on the assessment of working conditions (1,480). A high level of satisfaction with the work of nurses has a significant impact on providing better patient care as well as reducing the risk of professional burnout of nurses.
Work/household, transport, and leisure domains account for the sex gap in physical activity in Chile
Authors: Suárez-Reyes Mónica , Fernández-Verdejo Rodrigo
Published: September 2022

Background

Women usually have lower levels of moderate-vigorous physical activity (MVPA) than men. This sex gap can be accounted for by differences in MVPA in the work/household, transport, and/or leisure domains. Identifying where the differences lay in a context-specific manner may help close the gap. We aimed to compare MVPA by domain, and the relative contribution of each domain to total MVPA, between men and women in Chile.

Methods

We analyzed the cross-sectional National Health Survey of Chile 2016–2017 (n = 5,056, 64% women, ≥18 years old). MVPA was estimated with the Global Physical Activity Questionnaire. MVPA was expressed in MET × min/week, and the relative contribution to total MVPA by each domain was expressed as percentage. Analyses were conducted including all participants, and also including participants reporting >0 MET × min/week of MVPA (relative contributions can only be computed in the latter).

Results

Including all participants, women (vs. men) had lower MVPA (median [25–75th percentile]) for work/household (0 [0–960] vs. 0 [0–5,760] MET × min/week), for transport (360 [0–1,200] vs. 600 [0–1,680] MET × min/week), and for leisure domains (0 [0–0] vs. 0 [0–480] MET × min/week). Including only participants with >0 MET × min/week of MVPA, women (vs. men) had lower mean relative contributions to total MVPA from work/household (31.3 vs. 35.9%) and leisure domains (10.8 vs. 16.3%, respectively), but higher from the transport domain (57.9 vs. 47.8%).

Conclusion

In Chile, differences in all physical activity domains account for the sex gap in MVPA. Strategies to break job stereotypes, increase opportunities for leisure, and ease active transport are required to encourage MVPA in women.
Lay HIV counselors' knowledge and attitudes toward depression: A mixed-methods cross-sectional study at primary healthcare centers in Mozambique
Authors: Mandlate Flavio , Greene M. Claire , Pereira Luis F. , Sweetland Annika C. , Kokonya Donald , Duarte Cristiane S. , Cournos Francine , Oquendo Maria A. , Wainberg Milton L. , Sidat Mohsin , Sevene Esperança , Mello Marcelo F.
Published: September 2022

Introduction

Depression is the most common mental disorder among people living with HIV/AIDS and has a negative impact on HIV treatment outcomes. Training lay HIV counselors to identify and manage depression may contribute to improved patient access and adherence to treatment, and reduce stigma and discrimination among lay health workers toward both HIV and depression. The purpose of this study was to assess the current knowledge and attitudes of lay HIV counselors toward managing depression in primary care in Mozambique.

Methods

We conducted a mixed-methods cross-sectional study to assess depression-related knowledge and attitudes among lay HIV counselors in 13 primary healthcare facilities in Mozambique. We used the quantitative Depression Attitude Questionnaire (DAQ) scale, followed by open-ended questions to further explore three key DAQ domains: the nature of depression, treatment preferences, and professional attitudes or reactions.

Results

The sample included 107 participants (77.6% female, mean age: 32.3 years, sd = 7.4). Most (82.2%) had less than a high/technical school education. Findings suggested that some HIV counselors had knowledge of depression and described it as a cluster of psychological symptoms (e.g., deep sadness, anguish, apathy, isolation, and low self-esteem) sometimes leading to suicidal thoughts, or as a consequence of life stressors such as loss of a loved one, abuse, unemployment or physical illness, including being diagnosed with HIV infection. HIV counselors identified talking to trusted people about their problems, including family and/or counseling with a psychotherapist, as the best way for patients to deal with depression. While acknowledging challenges, counselors found working with patients with depression to be rewarding.

Conclusion

Lay health counselors identified HIV and psychosocial issues as key risk factors for depression. They believed that the treatment approach should focus on social support and psychotherapy.
Do patients with and survivors of COVID-19 benefit from telerehabilitation? A meta-analysis of randomized controlled trials
Authors: Huang Jiapeng , Fan Ye , Zhao Kehong , Yang Chunlan , Zhao Ziqi , Chen Yin , Yang Jiaen , Wang Tingting , Qu Yun
Published: September 2022

Background

Coronavirus disease 2019 (COVID-19) significantly impacts physical, psychological, and social functioning and reduces quality of life, which may persist for at least 6 months. Given the fact that COVID-19 is a highly infectious disease and therefore healthcare facilities may be sources of contagion, new methods avoiding face-to-face contact between healthcare workers and patients are urgently needed. Telerehabilitation is the provision of rehabilitation services to patients at a distanceviainformation and communication technologies. However, high-quality evidence of the efficacy of telerehabilitation for COVID-19 is still lacking. This meta-analysis aimed to investigate the efficacy of telerehabilitation for patients with and survivors of COVID-19.

Methods

We searched the Cochrane Library, EMBASE, Medline (viaPubMed), PEDro,ClinicalTrials.gov, and WHO International Clinical Trials Registry Platform from January 1st, 2020 to April 30th, 2022 for randomized controlled trials published in English, which aimed to evaluate the efficacy of telerehabilitation vs. face-to-face rehabilitation, usual care, or no treatment for COVID-19. Methodological quality and overall evidence quality of the included studies were assessed. The statistical reliability of the data was quantified using the trial sequential analysis.

Results

Seven randomized controlled trials with eight comparisons were included and all of them were used for meta-analysis. The meta-analyses of absolute values showed the superiority of telerehabilitation over no treatment or usual care for dyspnea (Borg scale: mean difference = −1.88, −2.37 to −1.39; Multidimensional dyspnea-12: mean difference = −3.70, −5.93 to −1.48), limb muscle strength (mean difference = 3.29; 2.12 to 4.47), ambulation capacity (standardized mean difference = 0.88; 0.62 to 1.14), and depression (mean difference = −5.68; −8.62 to −2.74). Significant improvement in these variables persisted in the meta-analyses of change scores. No significant difference was found in anxiety and quality of life. No severe adverse events were reported in any of the included studies.

Conclusions

Moderate- to very low-quality evidence demonstrates that telerehabilitation may be an effective and safe solution for patients with and survivors of COVID-19 in dyspnea, lower limb muscle strength, ambulation capacity, and depression. Further well-designed studies are required to evaluate the long-term effects, cost-effectiveness, and satisfaction in larger samples.
The relationship between anthropometric indicators and health-related quality of life in a community-based adult population: A cross-sectional study in Southern China
Authors: Fan Yu-Jun , Feng Yi-Jin , Meng Ya , Su Zhen-Zhen , Wang Pei-Xi
Published: September 2022

Background

This study was designed to analyze the relationship of waist circumference (WC), body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), relative fat mass (RFM), lipid accumulation product (LAP) and health-related quality of life (HRQoL) in the community-dwelling population of southern China and to explore the independent contribution of socio-demographic characteristics, number of chronic diseases and anthropometric indicators to HRQoL in that population.

Methods

This community-based cross-sectional survey studied 2,663 adults aged 18 years and older. HRQoL was assessed by the 3-level EuroQol 5-dimensional scale (EQ-5D-3L), and HRQoL were calculated using the Chinese EQ-5D-3L value set. The outcome variable was the EQ-5D-3L score (HRQoL). Cluster regression was used to analyse the independent contribution of each obesity indicator to HRQoL.

Results

A total of 2,663 people participated in this study, and their mean EQ-5D-3L score was 0.938 ± 0.072. In this study, according to the results of the one-way ANOVA, HRQoL was significantly different between the groups of WHtR, WHR, RFM and LAP, respectively. The independent contributions of socio-demographic factors, number of chronic diseases and anthropometric measures to HRQoL in the whole population accounted for 76.2, 7.9, and 15.9% of the total effect, respectively.

Conclusion

RFM and LAP were found to have a previously unreported negative impact on HRQoL in a community-dwelling population. In future studies, RFM and LAP could be used as new indicators of obesity to predict quality of life in humans.
Clinicopathological and surgical comparisons of differentiated thyroid cancer between China and the USA: A multicentered hospital-based study
Authors: Zhu Juan , Sun Kexin , Wang Jian , He Yutong , Li Daojuan , Liu Shuzheng , Huang Yunchao , Zhang Min , Song Bingbing , Liao Xianzhen , Liang He , Zhang Qian , Shi Mumu , Guo Lanwei , Zhou Yongchun , Lin Yanping , Lu Yanni , Tuo Jiyu , Xia Yafen , Sun Huixin , Xiao Haifan , Ji Yong , Yan Ci , Qiao Jinwan , Zeng Hongmei , Zheng Rongshou , Zhang Siwei , Liu Shaoyan , Chang Sheng , Wei Wenqiang
Published: September 2022

Background

Thyroid cancer (TC), was the fastest-rising tumor of all malignancies in the world and China, predominantly differentiated thyroid cancer (DTC). However, evidence on TC stage distribution and influencing factors of late-stage were limited in China.

Methods

We carried out a retrospective study and enrolled TC patients who were first diagnosed and hospitalized in 8 hospitals in China in 2017. Logistic regression was used to evaluate associations between influencing factors and DTC stage. We extracted eligible primary DTC records newly diagnosed in 2017 from the USA's Surveillance, Epidemiology, and End Results (SEER) database. We compared clinicopathological features and surgical treatment between our DTC records and those from the SEER database.

Results

A total of 1970 eligible patients were included, with 1861 DTC patients with known stage. Among patients ≥45 years old, males (OR = 1.76, 95%CI 1.17–2.65) and those with new rural cooperative medical scheme insurance (NCMS) (OR = 1.99, 95%CI 1.38–2.88) had higher risks of late-stage DTC (stage III-IV). Compared with SEER database, over-diagnosis is more common in China [more DTC patients with onset age< 45 years old (50.3 vs. 40.7%, P < 0.001), with early-stage (81.2 vs. 76.0%, P < 0.001), and with tumors<2cm (74.9 vs. 63.7%, P < 0.001)]. Compared with the USA, TC treatment is more conservative in China. The proportion of lobectomy in our database was significantly higher than that in the SEER database (41.3 vs. 17.0%, P < 0.001).

Conclusions

Unique risk factors are found to be associated with late-stage DTC in China. The differences in the aspect of clinicopathological features and surgical approaches between China and the USA indicate that potential over-diagnosis and over-surgery exist, and disparities on surgery extent may need further consideration. The findings provided references for other countries with similar patterns.
Clinical characteristics and pathogen spectra of parasitic infections in a tertiary hospital of Shanghai: A 13-year retrospective study
Authors: Zhang Jinming , Xu Jing , Tang Weiliang , Mo Ruidong , Shang Dabao , Lu Jie , Li Ziqiang , Wang Xiaolin , Shi Dongmei , Xie Qing , Xiang Xiaogang
Published: September 2022

Background

This study performed a follow-up investigation of parasitic infections and the evolution of the infection spectra in Shanghai and its surrounding areas in Eastern China. The current study was conducted in the Shanghai Ruijin Hospital, a tertiary hospital affiliated with Shanghai Jiao Tong University School of Medicine.

Methods

This retrospective investigation reviewed a total of 412 parasitic infections in patients admitted to the Department of Infectious Diseases, Ruijin Hospital from January 1, 2010 to July 31, 2022. Detailed information for these patients was retrieved from the Electronic Medical Record System. Analysis was performed using GraphPad Prism 5.0 and SPSS Statistics 26.

Results

Overall, 17 species of parasites were detected from the 412 admissions. Over the 13 years, the number of patients peaked in 2021 and food-born parasites (FBPs) were the primary species. During the most recent 5 years, Clonorchis sinensis, replacing Paragonimus westermani, has become the primary parasite detected among the patients, consistent with the observation that eating uncooked fish has turned into the most common route of transmission. Paragonimus westermani infections declined with age, but Cysticercus increased with age. The periods from the onset of symptoms to definite diagnosis for some patients infected with Sparganum mansoni, Paragonimus westermani, and Cysticercus were more than 6 months. Interestingly, eosinophilia was only detected in 51.83% of parasite-infected patients. In addition, superinfections of parasites were common in our study.

Conclusion

Our study demonstrates the transitional change in the prevalence of parasitic infection over the latest 13 years in a single center in Eastern China. The incidence of parasitic infections peaked in 2021, and the dominant parasitic species switched from a soil origin to foodborne. The direction for the diagnosis and prevention of parasitic infection among different age groups should alter according to age. It is difficult to diagnose parasitic infections and superinfections that occur in some patients. Thus, more sensitive and efficient detection methods should be developed. In addition, although eosinophilia and elevated IgE are still reliable indicators for initiating screening of parasitic infection, the development of novel parasitic diagnostic kits is still in urgent need for occult infection.
Palladium-coated kapton for use on dust detectors in low earth orbit: Performance under hypervelocity impact and atomic oxygen exposure
Authors: Dignam Aishling , Wozniakiewicz Penelope J. , Burchell Mark J. , Alesbrook Luke S. , Tighe Adrian , Suliga Agnieszka , Wessing Johanna , Kearsley Anton , Bridges John , Holt John , Howie Stuart , Peatman Libby , Fitzpatrick Dennis
Published: September 2022

Observation of dust and debris in the near Earth environment is a field of great commercial and scientific interest, vital to maximising the operational and commercial life-cycle of satellites and reducing risk to increasing numbers of astronauts in Low Earth Orbit (LEO). To this end, monitoring and assessment of the flux of particles is of paramount importance to the space industry and wider socio-economic interests that depend upon data products/services from orbital infrastructure. We have designed a passive space dust detector to investigate the dust environment in LEO—the Orbital Dust Impact Experiment (ODIE). ODIE is designed for deployment in LEO for ∼1 year, whereupon it would be returned to Earth for analysis of impact features generated by dust particles. The design emphasises the ability to distinguish between the orbital debris (OD) relating to human space activity and the naturally occurring micrometeoroid (MM) population at millimetre to submillimetre scales. ODIE is comprised of multiple Kapton foils, which have shown great potential to effectively preserve details of the impacting particles’ size and chemistry, with residue chemistry being used to interpret an origin (OD vs. MM). LEO is a harsh environment—the highly erosive effects of atomic oxygen damage Kapton foil—requiring the use of a protective coating. Common coatings available for Kapton (e.g., Al, SiO2, etc.) are problematic for subsequent analysis and interpretation of OD vs. MM origin, being a common elemental component of MM or OD, or having X-ray emission peaks overlapping with those of elements used to distinguish MM from OD. We thus propose palladium coatings as an alternative for this application. Here we report on the performance of palladium as a protective coating for a Kapton-based passive dust detector when exposed to atomic oxygen and impact. When subjected to impact, we observe that thicker coatings suffer delamination such that a coating of <50 nm is recommended. Analysis of atomic oxygen exposed samples shows a thin 10 nm coating of palladium significantly reduces the mass loss of Kapton, while coatings of 25 nm and over perform as well as or better than other commonly used coatings.
Cost-Effectiveness of Robotic vs. Laparoscopic Surgery for Different Surgical Procedures: Protocol for a Prospective, Multicentric Study (ROBOCOSTES)
Authors: Ielpo Benedetto , Podda Mauro , Burdio Fernando , Sanchez-Velazquez Patricia , Guerrero Maria-Alejandra , Nuñez Javier , Toledano Miguel , Morales-Conde Salvador , Mayol Julio , Lopez-Cano Manuel , Espín-Basany Eloy , Pellino Gianluca ,   The ROBOCOSTES Study Collaborators
Published: September 2022

Background

The studies which address the impact of costs of robotic vs. laparoscopic approach on quality of life (cost-effectiveness studies) are scares in general surgery.

Methods

The Spanish national study on cost-effectiveness differences among robotic and laparoscopic surgery (ROBOCOSTES) is designed as a prospective, multicentre, national, observational study. The aim is to determine in which procedures robotic surgery is more cost-effective than laparoscopic surgery. Several surgical operations and patient populations will be evaluated (distal pancreatectomy, gastrectomy, sleeve gastrectomy, inguinal hernioplasty, rectal resection for cancer, Heller cardiomiotomy and Nissen procedure).

Discussion

The results of this study will demonstrate which treatment (laparoscopic or robotic) and in which population is more cost-effective. This study will also assess the impact of previous surgical experience on main outcomes.
A Survey on Fault Diagnosis of Rolling Bearings
Authors: Peng Bo , Bi Ying , Xue Bing , Zhang Mengjie , Wan Shuting
Published: September 2022

The failure of a rolling bearing may cause the shutdown of mechanical equipment and even induce catastrophic accidents, resulting in tremendous economic losses and a severely negative impact on society. Fault diagnosis of rolling bearings becomes an important topic with much attention from researchers and industrial pioneers. There are an increasing number of publications on this topic. However, there is a lack of a comprehensive survey of existing works from the perspectives of fault detection and fault type recognition in rolling bearings using vibration signals. Therefore, this paper reviews recent fault detection and fault type recognition methods using vibration signals. First, it provides an overview of fault diagnosis of rolling bearings and typical fault types. Then, existing fault diagnosis methods are categorized into fault detection methods and fault type recognition methods, which are separately revised and discussed. Finally, a summary of existing datasets, limitations/challenges of existing methods, and future directions are presented to provide more guidance for researchers who are interested in this field. Overall, this survey paper conducts a review and analysis of the methods used to diagnose rolling bearing faults and provide comprehensive guidance for researchers in this field.
Compensation of Modeling Errors for the Aeroacoustic Inverse Problem with Tools from Deep Learning
Authors: Raumer Hans-Georg , Ernst Daniel , Spehr Carsten
Published: September 2022

In the field of aeroacoustic source imaging, one seeks to reconstruct acoustic source powers from microphone array measurements. For most setups, one cannot expect a perfect reconstruction. The main effects that contribute to this reconstruction error are data noise and modeling errors. While the data noise is accounted for in most advanced reconstruction methods, e.g., by a proper regularization strategy, the modeling error is usually neglected. This article proposes an approach that extends regularized inverse methods with a mechanism that takes the modeling error into account. The presented algorithmic framework utilizes the representation of the Fast Iterative Shrinkage Thresholding Algorithm (FISTA) algorithm by a neural network and uses standard gradient schemes from the field of deep learning. It is directly applicable to a single measurement, i.e., a prior training phase on previously generated data is not required. The capabilities of the method are illustrated by several numerical examples.
Unravelling the Efficient Applications of Zinc and Selenium for Mitigation of Abiotic Stresses in Plants
Authors: Ganguly Retwika , Sarkar Anik , Dasgupta Disha , Acharya Krishnendu , Keswani Chetan , Popova Victoria , Minkina Tatiana , Maksimov Aleksey Yu , Chakraborty Nilanjan
Published: September 2022

Abiotic stress factors are considered a serious threat to various growth parameters of crop plants. Stressors such as drought, salinity, and heavy metals (HMs) hamper the chlorophyll content in plants, resulting in low photosynthesis, hinder the integrity of cell membranes, reduce biomass, and overall growth and development of crops which ultimately results in the sharp decline of crop yield. Under such stressful conditions, various strategies are employed to overcome hazardous effects. Application of Zinc (Zn) or Selenium (Se) in different forms is an effective way to alleviate the abiotic stresses in plants. Zn and Se play a pivotal role in enhancing the chlorophyll level to improve photosynthesis, reducing oxidative stress by limiting reactive oxygen species (ROS) production, controlling HMs absorption by plant roots and their accumulation in the plant body, maintaining homeostasis, and alleviating all the detrimental effects caused by abiotic stress factors. The current review is focused on the usefulness of Zn and Se application, their uptake, sensitization, and different defence mechanisms to relieve adverse effects of abiotic stresses (such as drought, salinity, and HMs) on crops. In this connection, research gaps have also been highlighted.
Using RGB Imaging, Optimized Three-Band Spectral Indices, and a Decision Tree Model to Assess Orange Fruit Quality
Authors: Galal Hoda , Elsayed Salah , Elsherbiny Osama , Allam Aida , Farouk Mohamed
Published: September 2022

Point samples and laboratory testing have historically been used to evaluate fruit quality criteria. Although this method is precise, it is slow, expensive, and destructive, making it unsuitable for large-scale monitoring of these parameters. The main objective of this research was to develop a non-invasive protocol by combining color RGB indices (CIs) and previously published and newly developed three-band spectral reflectance indices (SRIs) with a decision tree (DT) model to evaluate the fruit quality parameters of navel orange. These parameters were brightness (L*), red–green (a*), blue–yellow (b*), chlorophyll meter (Chlm), total soluble solids (TSS), and TSS/acid ratio. The characteristics of fruit quality of navel orange samples were measured at various stages of ripening. The outcomes demonstrated that at various levels of ripening, the fruit quality parameters, RGB imaging indices, and published and newly developed three-band SRIs differed. The newly developed three-band SRIs based on the wavelengths of blue, green, red, red-edge, and NIR are most effective for estimating the six measured parameters in this study. For example, NDI574,592,724, NDI572,584,724, and NDI574,722,590 had the largest R2 value (0.90) with L*, whereas NDI526,664,700 and NDI524,700,664 exhibited the highest R2 value (0.97) with a*. Moreover, integrating CIs and SRIs with the DT model has provided a potentially useful tool for the accurate measurement of the six studied parameters. For instance, the DT-SRIs-CIs-30 model performed better in terms of measuring a* using 30 various indices. The R2 value was 0.98 and RMSE = 1.121 in the cross-validation, while R2 value was 0.964 and RMSE = 2.604 in the test set. Otherwise, based on the fusion of five various indices, the DT-SRIs-CIs-5 model was the most precise for recognizing b* (R2 = 0.957 and 0.929, with RMSE = 1.713 and 3.309 for cross-validation and test set, respectively). Overall, this work proves that integrating the different characteristics of proximal reflectance sensing systems such as color RGB indices and SRIs via the DT model may be considered a reliable instrument for evaluating the quality of different fruits.
Content of Heavy Metals in the Lichens of Winter Reindeer Pastures of the Timan and Bolshezemelskaya Tundras
Authors: Menshakova Marija , Huber Miłosz , Gainanova Ramziya , Surovets Valeriia , Moiseeva Nina , Nizikova Anastasiia , Mashinets Marina
Published: September 2022

This article is devoted to the study of the contents of zinc, cadmium, copper, and lead in lichen in the pastures of reindeer studs in the Timan and Bolshezemelskaya tundras. These areas are located in the Arctic part of Russia, to the west of the Polar Urals. These are areas where carbonate and sandstone rocks of the Permian–Mesozoic age dominate under the soil cover, as well as older deposits located in the western part of the research area (dated to the Cambrian–Devonian period). In these rocks, there is mineralization with metals, including copper. Research carried out in 2018 showed that in the surface layer of lichens, the concentration of metals was assessed differently in the upper, middle, and lower parts of the hill. On this basis, it was possible to identify clean and contaminated pastures. The high copper content in some pastures can be explained by the migration of metal ions from the parent rock. Due to the similar ionic radius of copper and the higher electrocativity in relation to zinc, the metal was probably displaced in the lichen. The observed concentrations can cause high levels of metals in the tissues and organs of deer. A high content of metals in lichens was found in samples collected near industrial enterprises, as well as at a considerable distance from them. At the same time, lichens at some locations near boiler houses or oil rigs are quite pure. In this regard, it has been suggested that the source of pollutants can be either natural factors (copper-bearing sands) or the ingress of metals from a remotely located source.
Exploring Antimicrobial Stewardship Influential Interventions on Improving Antibiotic Utilization in Outpatient and Inpatient Settings: A Systematic Review and Meta-Analysis
Authors: Sadeq Ahmed A. , Hasan Syed Shahzad , AbouKhater Noha , Conway Barbara R. , Abdelsalam Abeer E. , Shamseddine Jinan M. , Babiker Zahir Osman Eltahir , Nsutebu Emmanuel Fru , Bond Stuart E. , Aldeyab Mamoon A.
Published: September 2022

Antimicrobial stewardship interventions are targeted efforts by healthcare organizations to optimize antimicrobial use in clinical practice. The study aimed to explore effective interventions in improving antimicrobial use in hospitals. Literature was systemically searched for interventional studies through PubMed, CINAHL, and Scopus databases that were published in the period between January 2010 to April 2022. A random-effects model was used to pool and evaluate data from eligible studies that reported antimicrobial stewardship (AMS) interventions in outpatient and inpatient settings. Pooled estimates presented as proportions and standardized mean differences. Forty-eight articles were included in this review: 32 in inpatient and 16 in outpatient settings. Seventeen interventions have been identified, and eight outcomes have been targeted. AMS interventions improved clinical, microbiological, and cost outcomes in most studies. When comparing non-intervention with intervention groups using meta-analysis, there was an insignificant reduction in length of stay (MD: −0.99; 95% CI: −2.38, 0.39) and a significant reduction in antibiotics’ days of therapy (MD: −2.73; 95% CI: −3.92, −1.54). There were noticeable reductions in readmissions, mortality rates, and antibiotic prescriptions post antimicrobial stewardship multi-disciplinary team (AMS-MDT) interventions. Studies that involved a pharmacist as part of the AMS-MDT showed more significant improvement in measured outcomes than the studies that did not involve a pharmacist.
Local Treatment of Driveline Infection with Bacteriophages
Authors: Püschel Anja , Skusa Romy , Bollensdorf Antonia , Gross Justus
Published: September 2022

Drive line infections (DLI) are common infectious complications after left ventricular assist devices (LVAD) implantation. In case of severe or persistent infections, when conservative management fails, the exchange of the total LVAD may become necessary. We present a case of successful treatment of DL infection with a combination of antibiotics, debridement and local bacteriophage treatment.
Extended-Spectrum Beta-Lactamases Producing Enterobacteriaceae in the USA Dairy Cattle Farms and Implications for Public Health
Authors: Gelalcha Benti Deresa , Kerro Dego Oudessa
Published: September 2022

Antimicrobial resistance (AMR) is one of the top global health threats of the 21th century. Recent studies are increasingly reporting the rise in extended-spectrum beta-lactamases producing Enterobacteriaceae (ESBLs-Ent) in dairy cattle and humans in the USA. The causes of the increased prevalence of ESBLs-Ent infections in humans and commensal ESBLs-Ent in dairy cattle farms are mostly unknown. However, the extensive use of beta-lactam antibiotics, especially third-generation cephalosporins (3GCs) in dairy farms and human health, can be implicated as a major driver for the rise in ESBLs-Ent. The rise in ESBLs-Ent, particularly ESBLs-Escherichia coli and ESBLs-Klebsiella species in the USA dairy cattle is not only an animal health issue but also a serious public health concern. The ESBLs-E. coli and -Klebsiella spp. can be transmitted to humans through direct contact with carrier animals or indirectly through the food chain or via the environment. The USA Centers for Disease Control and Prevention reports also showed continuous increase in community-associated human infections caused by ESBLs-Ent. Some studies attributed the elevated prevalence of ESBLs-Ent infections in humans to the frequent use of 3GCs in dairy farms. However, the status of ESBLs-Ent in dairy cattle and their contribution to human infections caused by ESBLs-producing enteric bacteria in the USA is the subject of further study. The aims of this review are to give in-depth insights into the status of ESBL-Ent in the USA dairy farms and its implication for public health and to highlight some critical research gaps that need to be addressed.