bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Nanocellulose to Produce Water-Based Conductive Inks with Ag NPs for Printed Electronics
Authors: Martinez-Crespiera Sandra , Pepió-Tàrrega Belén , González-Gil Rosa M. , Cecilia-Morillo Francisco , Palmer Javier , Escobar Ana M. , Beneitez-Álvarez Sirio , Abitbol Tiffany , Fall Andreas , Aulin Christian , Nevo Yuval , Beni Valerio , Tolin Enrico , Bahr Achim
Published: March 2022

The need for more sustainable printed electronics has emerged in the past years. Due to this, the use of nanocellulose (NC) extracted from cellulose has recently been demonstrated to provide interesting materials such as functional inks and transparent flexible films due to its properties. Its high specific surface area together with the high content of reactive hydroxyl groups provide a highly tailorable surface chemistry with applications in ink formulations as a stabilizing, capping, binding and templating agent. Moreover, NC mechanical, physical and thermal properties (high strength, low porosity and high thermal stability, respectively) provide an excellent alternative for the currently used plastic films. In this work, we present a process for the production of water-based conductive inks that uses NC both as a template for silver nanoparticles (Ag NPs) formation and as an ink additive for ink formulation. The new inks present an electrical conductivity up to 2 × 106 S/m, which is in the range of current commercially available conductive inks. Finally, the new Ag NP/NC-based conductive inks have been tested to fabricate NFC antennas by screen-printing onto NC-coated paper, demonstrating to be operative.
Mitochondrial Implications in Cardiovascular Aging and Diseases: The Specific Role of Mitochondrial Dynamics and Shifts
Authors: Poznyak Anastasia V. , Kirichenko Tatiana V. , Borisov Evgeny E. , Shakhpazyan Nikolay K. , Kartuesov Andrey G. , Orekhov Alexander N.
Published: March 2022

Cardiovascular disease has been, and remains, one of the leading causes of death in the modern world. The elderly are a particularly vulnerable group. The aging of the body is inevitably accompanied by the aging of all its systems, and the cardiovascular system is no exception. The aging of the cardiovascular system is a significant risk factor for the development of various diseases and pathologies, from atherosclerosis to ischemic stroke. Mitochondria, being the main supplier of energy necessary for the normal functioning of cells, play an important role in the proper functioning of the cardiovascular system. The functioning of each individual cell and the organism as a whole depends on their number, structure, and performance, as well as the correct operation of the system in removing non-functional mitochondria. In this review, we examine the role of mitochondria in the aging of the cardiovascular system, as well as in diseases (for example, atherosclerosis and ischemic stroke). We pay special attention to changes in mitochondrial dynamics since the shift in the balance between fission and fusion is one of the main factors associated with various cardiovascular pathologies.
Bioinspired Polydopamine Coatings Facilitate Attachment of Antimicrobial Peptidomimetics with Broad-Spectrum Antibacterial Activity
Authors: Browne Katrina , Kuppusamy Rajesh , Chen Renxun , Willcox Mark D. P. , Walsh William R. , Black David StC. , Kumar Naresh
Published: March 2022

The prevention and treatment of biofilm-mediated infections remains an unmet clinical need for medical devices. With the increasing prevalence of antibiotic-resistant infections, it is important that novel approaches are developed to prevent biofilms forming on implantable medical devices. This study presents a versatile and simple polydopamine surface coating technique for medical devices, using a new class of antibiotics—antimicrobial peptidomimetics. Their unique mechanism of action primes them for activity against antibiotic-resistant bacteria and makes them suitable for covalent attachment to medical devices. This study assesses the anti-biofilm activity of peptidomimetics, characterises the surface chemistry of peptidomimetic coatings, quantifies the antibacterial activity of coated surfaces and assesses the biocompatibility of these coated materials. X-ray photoelectron spectroscopy and water contact angle measurements were used to confirm the chemical modification of coated surfaces. The antibacterial activity of surfaces was quantified for S. aureus, E. coli and P. aeruginosa, with all peptidomimetic coatings showing the complete eradication of S. aureus on surfaces and variable activity for Gram-negative bacteria. Scanning electron microscopy confirmed the membrane disruption mechanism of peptidomimetic coatings against E. coli. Furthermore, peptidomimetic surfaces did not lyse red blood cells, which suggests these surfaces may be biocompatible with biological fluids such as blood. Overall, this study provides a simple and effective antibacterial coating strategy that can be applied to biomaterials to reduce biofilm-mediated infections.
The Impact of Sub-Millisecond Damage Fixation Kinetics on the In Vitro Sparing Effect at Ultra-High Dose Rate in UNIVERSE
Authors: Liew Hans , Mein Stewart , Tessonnier Thomas , Abdollahi Amir , Debus Jürgen , Dokic Ivana , Mairani Andrea
Published: March 2022

The impact of the exact temporal pulse structure on the potential cell and tissue sparing of ultra-high dose-rate irradiation applied in FLASH studies has gained increasing attention. A previous version of our biophysical mechanistic model (UNIVERSE: UNIfied and VERSatile bio response Engine), based on the oxygen depletion hypothesis, has been extended in this work by considering oxygen-dependent damage fixation dynamics on the sub-milliseconds scale and introducing an explicit implementation of the temporal pulse structure. The model successfully reproduces in vitro experimental data on the fast kinetics of the oxygen effect in irradiated mammalian cells. The implemented changes result in a reduction in the assumed amount of oxygen depletion. Furthermore, its increase towards conventional dose-rates is parameterized based on experimental data from the literature. A recalculation of previous benchmarks shows that the model retains its predictive power, while the assumed amount of depleted oxygen approaches measured values. The updated UNIVERSE could be used to investigate the impact of different combinations of pulse structure parameters (e.g., dose per pulse, pulse frequency, number of pulses, etc.), thereby aiding the optimization of potential clinical application and the development of suitable accelerators.
Mechanical-Stress-Related Epigenetic Regulation of ZIC1 Transcription Factor in the Etiology of Postmenopausal Osteoporosis
Authors: Datta Harish K. , Kringen Marianne K. , Tuck Stephen P. , Salpingidou Georgia , Olstad Ole K. , Gautvik Kaare M. , Cockell Simon J. , Gautvik Vigdis T. , Prediger Michael , Wu Jun Jie , Birch Mark A. , Reppe Sjur
Published: March 2022

Mechanical loading exerts a profound influence on bone density and architecture, but the exact mechanism is unknown. Our study shows that expression of the neurological transcriptional factor zinc finger of the cerebellum 1 (ZIC1) is markedly increased in trabecular bone biopsies in the lumbar spine compared with the iliac crest, skeletal sites of high and low mechanical stress, respectively. Human trabecular bone transcriptome analyses revealed a strong association between ZIC1 mRNA levels and gene transcripts characteristically associated with osteoblasts, osteocytes and osteoclasts. This supposition is supported by higher ZIC1 expression in iliac bone biopsies from postmenopausal women with osteoporosis compared with age-matched control subjects, as well as strongly significant inverse correlation between ZIC1 mRNA levels and BMI-adjusted bone mineral density (BMD) (Z-score). ZIC1 promoter methylation was decreased in mechanically loaded vertebral bone compared to unloaded normal iliac bone, and its mRNA levels correlated inversely with ZIC1 promoter methylation, thus linking mechanical stress to epigenetic control of gene expression. The findings were corroborated in cultures of rat osteoblast progenitors and osteoblast-like cells. This study demonstrates for the first time how skeletal epigenetic changes that are affected by mechanical forces give rise to marked alteration in bone cell transcriptional activity and translate to human bone pathophysiology.
Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?
Authors: Foulon Arthur , Rybarczyk Pierre , Jonckheere Nicolas , Brabencova Eva , Sevestre Henri , Ouadid-Ahidouch Halima , Rodat-Despoix Lise
Published: March 2022

Breast cancer is the leading cause of cancer death among women in worldwide and France. The disease prognosis and treatment differ from one breast cancer subtype to another, and the disease outcome depends on many prognostic factors. Deregulation of ion flux (especially Ca2+ flux) is involved in many pathophysiology processes, including carcinogenesis. Inside the cell, the inositol-trisphosphate receptor (IP3R) is a major player in the regulation of the Ca2+ flux from the endoplasmic reticulum to the cytoplasm. The IP3Rs (and particularly the IP3R3 subtype) are known to be involved in proliferation, migration, and invasion processes in breast cancer cell lines. The objective of the present study was to evaluate the potential value of IP3Rs as prognostic biomarkers in breast cancer. We found that expression levels of IP3R3 and IP3R1 (but not IP3R2) were significantly higher in invasive breast cancer of no special type than in non-tumor tissue from the same patient. However, the IP3R3 subtype was expressed more strongly than the IP3R1 and IP3R2 subtypes. Furthermore, the expression of IP3R3 (but not of IP3R1 or IP3R2) was positively correlated with prognostic factors such as tumor size, regional node invasion, histologic grade, proliferation index, and hormone receptor status. In an analysis of public databases, we found that all IP3Rs types are significantly associated with overall survival and progression-free survival in patients with breast cancer. We conclude that relative to the other two IP3R subtypes, IP3R3 expression is upregulated in breast cancer and is correlated with prognostic factors.
A Possible Pathogenic PSEN2 Gly56Ser Mutation in a Korean Patient with Early-Onset Alzheimer’s Disease
Authors: Shim Kyu-Hwan , Kang Min-Ju , Bae Heewon , Kim Danyeong , Park Jiwon , An Seong-Soo , Jeong Da-Eun
Published: March 2022

Early-onset Alzheimer’s disease (EOAD) is characterized by the presence of neurological symptoms in patients with Alzheimer’s disease (AD) before 65 years of age. Mutations in pathological genes, including amyloid protein precursor (APP), presenilin-1 (PSEN1), and presenilin-2 (PSEN2), were associated with EOAD. Seventy-six mutations in PSEN2 have been found around the world, which could affect the activity of γ-secretase in amyloid beta processing. Here, a heterozygous PSEN2 point mutation from G to A nucleotide change at position 166 (codon 56; c.166G>A, Gly56Ser) was identified in a 64-year-old Korean female with AD with progressive cognitive memory impairment for the 4 years prior to the hospital visit. Hippocampal atrophy was observed from magnetic resonance imaging-based neuroimaging analyses. Temporal and parietal cortex hypometabolisms were identified using fluorodeoxyglucose positron emission tomography. This mutation was at the N-terminal portion of the presenilin 2 protein on the cytosolic side. Therefore, the serine substitution may have promoted AD pathogenesis by perturbing to the mutation region through altered phosphorylation of presenilin. In silico analysis revealed that the mutation altered protein bulkiness with increased hydrophilicity and reduced flexibility of the mutated region of the protein. Structural changes were likely caused by intramolecular interactions between serine and other residues, which may have affected APP processing. The functional study will clarify the pathogenicity of the mutation in the future.
Micro-Osteoperforations Induce TNF-α Expression and Accelerate Orthodontic Tooth Movement via TNF-α-Responsive Stromal Cells
Authors: Kinjo Ria , Kitaura Hideki , Ogawa Saika , Ohori Fumitoshi , Noguchi Takahiro , Marahleh Aseel , Nara Yasuhiko , Pramusita Adya , Ma Jinghan , Kanou Kayoko , Mizoguchi Itaru
Published: March 2022

Micro-osteoperforations (MOPs) have been reported to accelerate orthodontic tooth movement (OTM), and tumor necrosis factor (TNF)-α has been reported to play a crucial role in OTM. In this report, the influence of MOPs during OTM was analyzed. We evaluated the expression of TNF-α with and without MOPs by RT-PCR analysis. A Ni-Ti closed coil spring was fixed between the maxillary left first molar and the incisors as an OTM mouse model to move the first molar in the mesial direction. MOPs were prepared on the lingual side and mesial side of the upper first molars. Furthermore, to investigate the target cell of TNF-α for osteoclast formation during OTM with MOPs in vivo, we created four types of chimeric mice in which bone marrow of wild-type (WT) or TNF receptor 1- and 2-deficient mice (KO) was transplanted into lethally irradiated WT or KO mice. The results showed that MOPs increased TNF-α expression, the distance of tooth movement and osteoclast formation significantly. Furthermore, mice with TNF-α-responsive stromal cells showed a significant increase in tooth movement and number of osteoclasts by MOPs. We conclude that MOPs increase TNF-α expression, and tooth movement is dependent on TNF-α-responsive stromal cells.
Operational Rule Extraction and Construction Based on Task Scenario Analysis
Authors: Zhao Xinye , Wang Chao , Cui Peng , Sun Guangming
Published: March 2022

Changes in the information age have induced the necessity for a more efficient and effective self-decision-making requirement. A method of extracting and constructing naval operations decision-making rules based on scenario analysis is proposed. The template specifications of Event Condition Action (ECA) rules are defined, and a consistency detection method of ECA rules based on SWRL is proposed. The logical relationships and state transitions of the naval operational process is analyzed in detail, and the association of objects, events, and behaviors is realized. Finally, the operation of the proposed methods is illustrated through an example process, showing the method can effectively solve the problems of self-decision-making rule extraction and construction among naval battlefield decision environment, and avoid relying on artificial intelligence, which may have brought some uncertain factors.
A Method for Determining the Shape Similarity of Complex Three-Dimensional Structures to Aid Decay Restoration and Digitization Error Correction
Authors: Vasic Iva , Quattrini Ramona , Pierdicca Roberto , Frontoni Emanuele , Vasic Bata
Published: March 2022

This paper introduces a new method for determining the shape similarity of complex three-dimensional (3D) mesh structures based on extracting a vector of important vertices, ordered according to a matrix of their most important geometrical and topological features. The correlation of ordered matrix vectors is combined with perceptual definition of salient regions in order to aid detection, distinguishing, measurement and restoration of real degradation and digitization errors. The case study is the digital 3D structure of the Camino Degli Angeli, in the Urbino’s Ducal Palace, acquired by the structure from motion (SfM) technique. In order to obtain an accurate, featured representation of the matching shape, the strong mesh processing computations are performed over the mesh surface while preserving real shape and geometric structure. In addition to perceptually based feature ranking, the new theoretical approach for ranking the evaluation criteria by employing neural networks (NNs) has been proposed to reduce the probability of deleting shape points, subject to optimization. Numerical analysis and simulations in combination with the developed virtual reality (VR) application serve as an assurance to restoration specialists providing visual and feature-based comparison of damaged parts with correct similar examples. The procedure also distinguishes mesh irregularities resulting from the photogrammetry process.
No Sting in the Tail for Sterile Bisex Queensland Fruit Fly (Bactrocera tryoni Froggatt) Release Programs
Authors: Reynolds Olivia L. , Collins Damian , Dominiak Bernard C. , Osborne Terry
Published: March 2022

Global markets do not tolerate the presence of fruit fly (Tephritidae) in horticultural produce. A key method of control for tephritidae pests, is the sterile insect technique (SIT). Several countries release a bisex strain, i.e., males and females, however the sterile male is the only sex which contributes to wild population declines when released en masse. In commercial orchards, there are concerns that sterile females released as part of bisex strains, may oviposit, i.e., ‘sting’ and cause damage to fruit, rendering it unmarketable. Australia has released a bisex strain of sterile Queensland fruit fly, Bactrocera tryoni Froggatt, for several decades to suppress wild pest populations, particularly in peri-urban and urban environments. Here, we assessed fruit damage in two commercially grown stone fruit orchards where bisex sterile B. tryoni were released, and in an orchard that did not receive sterile flies. The number of detected stings were higher in only one SIT release orchard, compared with the control; however, there was no difference between SIT and control orchards in the number of larvae detected. We showed that there is no evidence that sterile female B. tryoni released in large numbers caused stings, or damage that led to downgraded or unsaleable fruit. The bisex strain of sterile B. tryoni is recommended for use in commercial stone-fruit orchards, under the conditions in which this trial was conducted.
AMD Genomics: Non-Coding RNAs as Biomarkers and Therapeutic Targets
Authors: Zhang Charles , Owen Leah A. , Lillvis John H. , Zhang Sarah X. , Kim Ivana K. , DeAngelis Margaret M.
Published: March 2022

Age-related macular degeneration (AMD) is a progressive neurodegenerative disease that is the world’s leading cause of blindness in the aging population. Although the clinical stages and forms of AMD have been elucidated, more specific prognostic tools are required to determine when patients with early and intermediate AMD will progress into the advanced stages of AMD. Another challenge in the field has been the appropriate development of therapies for intermediate AMD and advanced atrophic AMD. After numerous negative clinical trials, an anti-C5 agent and anti-C3 agent have recently shown promising results in phase 3 clinical trials, in terms of slowing the growth of geographic atrophy, an advanced form of AMD. Interestingly, both drugs appear to be associated with an increased incidence of wet AMD, another advanced form of the disease, and will require frequent intravitreal injections. Certainly, there remains a need for other therapeutic agents with the potential to prevent progression to advanced stages of the disease. Investigation of the role and clinical utility of non-coding RNAs (ncRNAs) is a major advancement in biology that has only been minimally applied to AMD. In the following review, we discuss the clinical relevance of ncRNAs in AMD as both biomarkers and therapeutic targets.
Impact of the Organizational Model Adopted during the COVID-19 Pandemic on the Perceived Safety of Intensive Care Unit Staff
Authors: Conoscenti Elena , Campanella Maria , Sala Antonino , Di Stefano Maria Cristina , Vinci Dario , Lombardo Rosario , Arena Giuseppe , Ginestra Angelo , Fiolo Rosario , Tuzzolino Fabio , Ippolito Alessia , Martucci Gennaro , Enea Giuseppe , Luca Angelo
Published: March 2022

Background: The SARS-CoV-2 pandemic had a devastating health, social, and economic effect on the population. Organizational, technical and structural operations aimed at protecting staff, outpatients and inpatients were implemented in an Italian hospital with a COVID-19 dedicated intensive care unit. The impact of the organizational model adopted on the perceived safety among staff was evaluated. Methods: Descriptive, structured and voluntary, anonymous, non-funded, self-administered cross-sectional surveys on the impact of the organizational model adopted during COVID-19 on the perceived safety among staff. Results: Response rate to the survey was 67.4% (153 completed surveys). A total of 91 (59%) of respondents had more than three years of ICU experience, while 16 (10%) were employed for less than one year. Group stratification according to profession: 74 nurses (48%); 12 medical-doctors (7%); 11 physiotherapists (7%); 35 nurses-aides (22%); 5 radiology-technicians (3%); 3 housekeeping (1%); 13 other (8%). The organizational model implemented at ISMETT made them feel safe during their workday. A total of 113 (84%) agreed or strongly agreed with the sense of security resulting from the implemented measures. A vast majority of respondents perceived COVID-19 as a dangerous and deadly disease (94%) not only for themselves but even more as vectors towards their families (79%). A total of 55% of staff took isolation measures and moved away from their home by changing personal habits. The organizational model was perceived overall as appropriate (91%) to guarantee their health. Conclusion: The vast majority of respondents perceived the overall model applied during an unexpected, emergency situation as appropriate.
Long Term Impact of Epicardial Left Atrial Appendage Ligation on Systemic Hemostasis: LAA HOMEOSTASIS-2
Authors: Bartus Krzysztof , Kanuri Sri Harsha , Litwinowicz Radoslaw , Ali Elbey Mehmet , Natorska Joanna , Zabczyk Michal , Bartus Magdalena , Kapelak Boguslaw , Gopinnathannair Rakesh , Garg Jalaj , Turagam Mohit K. , Malecki Maciej T. , Lee Randall J. , Lakkireddy Dhanunjaya
Published: March 2022

Background: Recent data suggest that epicardial left atrial appendage closure (LAAC) is associated with several short-term neurohormonal effects. However, the long-term effects are currently unknown. Objective: To investigate the effects of percutaneous epicardial left atial appendage (LAA) exclusion using LARIAT on neurohormonal profiles at long-term follow-up. Methods: In a prospective single centre study, 60 patients with long-standing, persistent atrial fibrillation (AF) LARIAT were treated. The major hormones of the adrenergic system, renin-angiotensin-aldosterone system (RAAS), and natriuretic peptides were assessed before the intervention and at regular intervals during the following two years. Results: In patients with epicardial LAAC, atrial natriuretic peptide (ANP) levels were significantly increased from baseline at 24 h and decreased at 7 days, 1 month, and 3 months, while remaining unchanged at 12 and 24 months. Noradrenaline levels were significantly lower at 24 h, 7 days, 1 month, 6 months, 12 months, and 24 months, while epinephrine levels decreased significantly at 1 month, 6 months, 12 months, and 24 months. Plasma renin activity significantly decreased at 7 days, 1 month, 6 months, 12 months, and 24 months, while aldosterone levels significantly decreased at 6 months, 12 months, and 24 months. Endothelin-1 and vasopressin showed a significant increase and decrease, respectively, at 24 h, 7 days, 1 month, 6 months, 12 months, and 24 months. There was also a significant decrease in systolic and diastolic blood pressure at 3 months, 6 months, 1 year, and 2 years after the intervention. Conclusions: Epicardial LAAC in AF patients is associated with persistent neurohormonal changes favouring blood pressure reduction.
Estimating Renal Function Following Lung Transplantation
Authors: Hornum Mads , Houlind Morten Baltzer , Iversen Esben , Porrini Esteban , Luis-Lima Sergio , Oturai Peter , Iversen Martin , Bredahl Pia , Carlsen Jørn , Møller Christian Holdflood , Andersen Mads Jønsson , Feldt-Rasmussen Bo , Perch Michael
Published: March 2022

Background: Patients undergoing lung transplantation (LTx) experience a rapid decline in glomerular filtration rate (GFR) in the acute postoperative period. However, no prospective longitudinal studies directly comparing the performance of equations for estimating GFR in this patient population currently exist. Methods: In total, 32 patients undergoing LTx met the study criteria. At pre-LTx and 1-, 3-, and 12-weeks post-LTx, GFR was determined by 51Cr-EDTA and by equations for estimating GFR based on plasma (P)-Creatinine, P-Cystatin C, or a combination of both. Results: Measured GFR declined from 98.0 mL/min/1.73 m2 at pre-LTx to 54.1 mL/min/1.73 m2 at 12-weeks post-LTx. Equations based on P-Creatinine underestimated GFR decline after LTx, whereas equations based on P-Cystatin C overestimated this decline. Overall, the 2021 CKD-EPI combination equation had the lowest bias and highest precision at both pre-LTx and post-LTx. Conclusions: Caution must be applied when interpreting renal function based on equations for estimating GFR in the acute postoperative period following LTx. Simplified methods for measuring GFR may allow for more widespread use of measured GFR in this vulnerable patient population.
Adherence and Persistence to Biological Drugs for Psoriasis: Systematic Review with Meta-Analysis
Authors: Piragine Eugenia , Petri Davide , Martelli Alma , Janowska Agata , Dini Valentina , Romanelli Marco , Calderone Vincenzo , Lucenteforte Ersilia
Published: March 2022

Despite the large number of biologics currently available for moderate-to-severe psoriasis, poor adherence and persistence to therapy represent the main issues for both the clinical and economic management of psoriasis. However, the data about adherence and persistence to biologics in psoriasis patients are conflicting. Our aim was to produce summary estimates of adherence and persistence to biologics in adult patients with psoriasis. We performed a systematic review and meta-analysis of observational studies, searching two databases (PubMed and Embase). Sixty-two records met the inclusion criteria, and a meta-analysis was conducted on fifty-five studies. Overall, the proportion of adherent and persistent patients to biological therapy was 0.61 (95% confidence interval: 0.48–0.73) and 0.63 (0.57–0.68), respectively. The highest proportions were found for ustekinumab, while the lowest ones were found for etanercept. The proportions of adherence and persistence to biological drugs in psoriasis patients are sub-optimal. Notably, both proportions largely differ between drugs, suggesting that a more rational use of biologics might ensure better management of psoriasis.
Two-Dimensional Nanomaterials beyond Graphene for Biomedical Applications
Authors: Derakhshi Maryam , Daemi Sahar , Shahini Pegah , Habibzadeh Afagh , Mostafavi Ebrahim , Ashkarran Ali Akbar
Published: March 2022

Two-dimensional (2D) nanomaterials (e.g., graphene) have shown to have a high potential in future biomedical applications due to their unique physicochemical properties such as unusual electrical conductivity, high biocompatibility, large surface area, and extraordinary thermal and mechanical properties. Although the potential of graphene as the most common 2D nanomaterials in biomedical applications has been extensively investigated, the practical use of other nanoengineered 2D materials beyond graphene such as transition metal dichalcogenides (TMDs), topological insulators (TIs), phosphorene, antimonene, bismuthene, metal–organic frameworks (MOFs) and MXenes for biomedical applications have not been appreciated so far. This review highlights not only the unique opportunities of 2D nanomaterials beyond graphene in various biomedical research areas such as bioelectronics, imaging, drug delivery, tissue engineering, and regenerative medicine but also addresses the risk factors and challenges ahead from the medical perspective and clinical translation of nanoengineered 2D materials. In conclusion, the perspectives and future roadmap of nanoengineered 2D materials beyond graphene are outlined for biomedical applications.
A Comparative Study of Two Bone Graft Substitutes—InterOss® Collagen and OCS-B Collagen®
Authors: Jain Gaurav , Blaauw Dylan , Chang Steve
Published: March 2022

Bone is a complex hierarchical tissue composed of organic and inorganic materials that provide structure, support, and protection to organs. However, there are some critical size defects that are unable to regenerate on their own and therefore require clinical repair. Bone graft substitutes allow repair by providing a temporary resorbable device. Among the common filler materials that aid in regeneration is hydroxyapatite particles of either animal or human origin which is used to fill or reconstruct periodontal and bony defects in the mouth. However, particulate graft substitutes suffer from localized migration away from the implantation site, necessitating the use of a barrier membrane. In this study, we designed InterOss Collagen, combining bovine hydroxyapatite granules with porcine-skin derived collagen to form a bone filler composite. Physiochemical properties of InterOss Collagen and a commercially available product, OsteoConductive Substitute-Bovine (OCS-B) Collagen, referred to as OCS-B Collagen, were examined. We found two bone graft substitutes to be mostly similar, though InterOss Collagen showed comparatively higher surface area and porosity. We conducted an in vivo study in rabbits to evaluate local tissue responses, percent material resorption and bone formation and showed that the two materials exhibited similar degradation profiles, inflammatory and healing responses following implantation. Based on these results, InterOss Collagen is a promising dental bone grafting material for periodontal and maxillofacial surgeries.
Rubinosporus auriporus gen. et sp. nov. (Boletaceae: Xerocomoideae) from Tropical Forests of Thailand, Producing Unusual Dark Ruby Spore Deposits
Authors: Vadthanarat Santhiti , Raspé Olivier , Lumyong Saisamorn
Published: March 2022

Rubinosporus, a new bolete genus from tropical forests of Thailand is introduced with R. auriporus as the type species. The genus is unique among Xerocomoideae in producing dark ruby spore deposits. It can be differentiated from all other Boletaceae genera by the following combination of characters: pileus surface evenly covered with matted tomentum; stipe surface with evenly scattered minute squamules; golden yellow tubular hymenophore, which is relatively thin especially when young; unchanging surfaces and context when bruised or cut; smooth, broadly ellipsoid basidiospores; and dark ruby spore deposits. The Boletaceae-wide and Xerocomoideae-wide phylogenetic analyses based on four-gene data sets (atp6, cox3, rpb2, and tef1) support Rubinosporus as monophyletic and places it in Boletaceae subfamily Xerocomoideae. Full descriptions and illustrations of the new genus and species are presented.
The Primacy of Secondary Things: A Sustained Scientific Dialogue on Three Edges of the Journalistic Field
Authors: Ferron Benjamin , Kotišová Johana , Smith Simon
Published: March 2022

While the sociology of journalism has traditionally granted epistemic privilege to the mainstream news media, professional elites and their dominant meta-discourse, there is a recent trend to research journalism from other points of view. Researchers have investigated alternative and community media, journalists with low visibility or legitimacy in the workplace, as well as heterodox conceptions of professional excellence. These studies shed new light on little studied sub-groups and practices. How can we integrate them into a sustained scientific dialogue between researchers? This paper presents a methodologically original attempt to do so by dialogically re-interrogating material from studies of three situations where journalism absorbs precarious and politicised agents in the field (media activists), new practices and tasks that need doing (online discussion administration) and unusual kinds of professional attributes (experiencing emotionality in crisis reporting). In each case journalism’s pursuit of professional autonomy is at stake, since conditions of production clash with established professional myths and practices or generate incompatibilities with institutionalised expectations. Our three-way exchange using the bridging/sensitising concept of edge focuses on the conversion or convertibility of external forms of capital (legitimacies, resources and experiences) into forms redeemable and tradeable in a professional field. It exhibits how forces external to the journalistic profession are (made) present in each case but refracted differently in each local configuration. We call for a more systematic and relational study of these kinds of localised refractions.
The Free Myocutaneous Tensor Fasciae Latae Flap—A Workhorse Flap for Sternal Defect Reconstruction: A Single-Center Experience
Authors: Bigdeli Amir Khosrow , Falkner Florian , Thomas Benjamin , Hundeshagen Gabriel , Mayer Simon Andreas , Risse Eva-Maria , Harhaus Leila , Gazyakan Emre , Kneser Ulrich , Radu Christian Andreas
Published: March 2022

Introduction: Deep sternal wound infections (DSWI) after cardiac surgery pose a significant challenge in reconstructive surgery. In this context, free flaps represent well-established options. The objective of this study was to investigate the clinical outcome after free myocutaneous tensor fasciae latae (TFL) flap reconstruction of sternal defects, with a special focus on surgical complications and donor-site morbidity. Methods: A retrospective chart review focused on patient demographics, operative details, and postoperative complications. Follow-up reexaminations included assessments of the range of motion and muscle strength at the donor-site. Patients completed the Quality of Life 36-item Short Form Health Survey (SF-36) as well as the Lower Extremity Functional Scale (LEFS) questionnaire and evaluated aesthetic and functional outcomes on a 6-point Likert scale. The Vancouver Scar Scale (VSS) and the Patient and Observer Scar Assessment Scales (POSAS) were used to rate scar appearance. Results: A total of 46 patients (mean age: 67 ± 11 years) underwent sternal defect reconstruction with free TFL flaps between January 2010 and March 2021. The mean defect size was 194 ± 43 cm2. The mean operation time was 387 ± 120 min with a flap ischemia time of 63 ± 16 min. Acute microvascular complications due to flap pedicle thromboses occurred in three patients (7%). All flaps could be salvaged without complete flap loss. Partial flap loss of the distal TFL portion was observed in three patients (7%). All three patients required additional reconstruction with pedicled or local flaps. Upon follow-up, the range of motion (hip joint extension/flexion (p = 0.73), abduction/adduction (p = 0.29), and internal/external rotation (p = 0.07)) and muscle strength at the donor-sites did not differ from the contralateral sides (p = 0.25). Patient assessments of aesthetic and functional outcomes, as well as the median SF-36 (physical component summary (44, range of 33 to 57)) and LEFS (54, range if 35 to 65), showed good results with respect to patient comorbidities. The median VSS (3, range of 2 to 7) and POSAS (24, range of 18 to 34) showed satisfactory scar quality and scar appearance. Conclusion: The free TFL flap is a reliable, effective, and, therefore, valuable option for the reconstruction of extensive sternal defects in critically ill patients suffering from DSWIs. In addition, the TFL flap shows satisfactory functional and aesthetic results at the donor-site.
Genetic Polymorphisms in a Familial Hypercholesterolemia Population from North-Eastern Europe
Authors: Maștaleru Alexandra , Cojocariu Sabina Alexandra , Oancea Andra , Constantin Maria Magdalena Leon , Roca Mihai , Zota Ioana Mădălina , Abdulan Irina , Rusu Cristina , Popescu Roxana , Antoci Lucian Mihai , Ciobanu Cristian Gabriel , Costache Alexandru Dan , Cojocaru Elena , Mitu Florin
Published: March 2022

(1) Background: Familial hypercholesterolemia (FH) is one of the most prevalent inherited metabolic disorders. The purpose of the study was to investigate the role in cardiovascular disease (CVD) of PAI-1, ACE, ApoB-100, MTHFR A1298C, and C677T. (2) Methods: From a group of 1499 patients, we included 52 patients diagnosed with FH phenotype and 17 patients in a control group. (3) Results: Most of the FH patients had multiple comorbidities compared to the control group, such as atherosclerosis (48.1% vs. 17.6%), atherosclerotic cardiovascular disease (ASCVD 32.7% vs. 11.8%), and metabolic syndrome (MetS, 40.4% vs. 11.8%). In total, 66.7% of the FH patients had PAI-1 4G/5G genotype and MetS. Between 4G/5G and 4G/4G, a statistically significant difference was observed (p = 0.013). FH patients with ApoB R3500Q polymorphism were correlated with ASCVD (p = 0.031). Both MTHFR C677T and A1298C polymorphisms had a significant correlation with gender, alcohol consumption, and smoking status. ACE polymorphism was associated with ATS in FH patients, statistically significant differences being observed between heterozygous and homozygous D genotype (p = 0.036) as well as between heterozygous and homozygous I genotype (p = 0.021). (4) Conclusions: A link between these polymorphisms was demonstrated in the FH group for ATS, ASCVD, and MetS.
Long-Term Comparative Study on the Local Tumour Control of Different Ablation Technologies in Primary and Secondary Liver Malignancies
Authors: Kovács Attila , Bischoff Peter , Haddad Hathal , Zhou Willi , Temming Susanne , Schäfer Andreas , Spallek Hannah , Kaupe Lucas , Kovács György , Pinkawa Michael
Published: March 2022

Purpose: To evaluate local tumour control (LTC) by local ablation techniques (LAT) in liver malignancies. Materials and methods: In patients treated with LAT between January 2013 and October 2020 target lesions were characterised by histology, dimensions in three spatial axes, volume, vascularisation and challenging (CL) location. LAT used were: Radiofrequency Ablation (RFA), Microwave Ablation (MWA), Cryoablation (CRYO), Electrochemotherapy (ECT), and Interstitial Brachytherapy (IBT). Results: 211 LAT were performed in 155 patients. Mean follow-up including MRI for all patients was 11 months. Lesions treated with ECT and IBT were significantly larger and significantly more often located in CL in comparison to RFA, MWA and CRYO. Best LTC (all data for 12 months are given below) resulted after RFA (93%), followed by ECT (81%), CRYO (70%), IBT (68%) and MWA (61%), and further, entity-related for HCC (93%), followed by CRC (83%) and BrC (72%), without statistically significant differences. LTC in hypovascular lesions was worse (64%), followed by intermediate (82% p = 0.01) and hypervascular lesions (92% p = 0.07). Neither diameter (<3 cm: 81%/3–6 cm: 74%/>6 cm: 70%), nor volume (<10 cm3: 80%/10–20 cm3: 86%/>20 cm3: 67%), nor CL (75% in CL vs. 80% in non CL) had a significant impact on LTC. In CL, best LTC resulted after ECT (76%) and IBT (76%). Conclusion: With suitable LAT, similarly good local tumour control can be achieved regardless of lesion size and location of the target.
ELBA-IoT: An Ensemble Learning Model for Botnet Attack Detection in IoT Networks
Authors: Abu Al-Haija Qasem , Al-Dala’ien Mu’awya
Published: March 2022

Due to the prompt expansion and development of intelligent systems and autonomous, energy-aware sensing devices, the Internet of Things (IoT) has remarkably grown and obstructed nearly all applications in our daily life. However, constraints in computation, storage, and communication capabilities of IoT devices has led to an increase in IoT-based botnet attacks. To mitigate this threat, there is a need for a lightweight and anomaly-based detection system that can build profiles for normal and malicious activities over IoT networks. In this paper, we propose an ensemble learning model for botnet attack detection in IoT networks called ELBA-IoT that profiles behavior features of IoT networks and uses ensemble learning to identify anomalous network traffic from compromised IoT devices. In addition, our IoT-based botnet detection approach characterizes the evaluation of three different machine learning techniques that belong to decision tree techniques (AdaBoosted, RUSBoosted, and bagged). To evaluate ELBA-IoT, we used the N-BaIoT-2021 dataset, which comprises records of both normal IoT network traffic and botnet attack traffic of infected IoT devices. The experimental results demonstrate that our proposed ELBA-IoT can detect the botnet attacks launched from the compromised IoT devices with high detection accuracy (99.6%) and low inference overhead (40 µ-seconds). We also contrast ELBA-IoT results with other state-of-the-art results and demonstrate that ELBA-IoT is superior.
Changes in Soil Quality of an Urban Wetland as a Result of Anthropogenic Disturbance
Authors: Clunes John , Valle Susana , Dörner Jose , Campos Marco , Medina Jorge , Zuern Sarah , Lagos Lorena
Published: March 2022

Urban wetland soil provides ecosystem services (ES) through their functions. Changes in soil properties due to anthropogenic disturbances lead to a loss of soil quality. The aim of this research was to evaluate the effect of nearby anthropic disturbance on the chemical, physical and biological properties of the urban wetland soil. Soil samples were collected from four sites (P1, P2, P3 and P4) located in the Angachilla urban wetland, Chile, according to the magnitude of anthropogenic disturbance. An assessment of the physical and chemical properties of the soil profile was carried out in two sites, P1 and P4. Additionally, chemical and biological properties of the soil were evaluated in the four sites selected. Results from the soil profiles showed that Hz1 of P4 had a higher levels of soil fertility as a result of low anthropogenic disturbance in contrast to Hz1 of P1 (p < 0.05). Relevant differences among sites were observed for P-Olsen, pH NaF, nosZ gene, Nitrate and Na (PC1: 50.5%). Composition of the soil bacterial community in P1 and P4 showed higher richness and diversity. Anthropogenic disturbance on the urban wetland soil leads to a loss of the soil’s organic horizon, as well as its soil quality and, subsequently, its capacity to provide ES through its functions.
Tree Advisor: A Novel Woody Plant Selection Tool to Support Multifunctional Objectives
Authors: Bentrup Gary , Dosskey Michael G.
Published: March 2022

Purposefully planted trees and shrubs can provide multiple benefits when appropriately planned and designed. Tools to help select species that will function more effectively than other species for ecosystem services, production, and aesthetic purposes are generally lacking. To address this challenge, we developed an interactive plant selection tool entitled Tree Advisor that rates woody species for a wide range of different purposes based on plant attributes. In this prototype decision support tool, 90 species of trees and shrubs are rated for 14 different purposes in the northern and central Great Plains region of the United States. A rating algorithm was developed based on the scientific literature regarding plant functions and related attributes that determine relative performance of a species for each purpose. User input and best practices for developing effective decision support tools informed the tool development process. Based on user feedback, the tool supports multifunctional planning and enables a user to quickly develop a short list of the better species to use which can then be refined by the user based on suitability under local site conditions, commercial availability, and availability of locally adapted cultivars and hybrids. This tool development approach can serve as a model for producing multifunctional woody plant selection tools for other ecoregions.
Quantifying Land Fragmentation in Northern Irish Cattle Enterprises
Authors: Milne Georgina , Byrne Andrew William , Campbell Emma , Graham Jordon , McGrath John , Kirke Raymond , McMaster Wilma , Zimmermann Jesko , Adenuga Adewale Henry
Published: March 2022

Farmland fragmentation is considered to be a defining feature of Northern Ireland’s (NI) agricultural landscape, influencing agricultural efficiency, productivity, and the spread of livestock diseases. Despite this, the full extent of farmland fragmentation in cattle farms in NI is not well understood, and little is known of how farmland fragmentation either influences, or is influenced by, different animal production types. Here, we describe and quantify farmland fragmentation in cattle farms for all of NI, using GIS processing of land parcel data to associate individual parcels with data on the cattle business associated with the land. We found that 35% of farms consisted of five or more fragments, with dairy farms associated with greater levels of farmland fragmentation, fragment dispersal and contact with contiguous neighbours compared to other production types. The elevated levels of farmland fragmentation in dairy production compared to non-dairy, may be associated with the recent expansion of dairy farms by land acquisition, following the abolition of the milk quota system in 2015. The comparatively high levels of farmland fragmentation observed in NI cattle farms may also have important implications for agricultural productivity and epidemiology alike. Whilst highly connected pastures could facilitate the dissemination of disease, highly fragmented land could also hamper productivity via diseconomies of scale, such as preventing the increase of herd sizes or additionally, adding to farm costs by increasing the complexity of herd management.
Research into Satisfaction with Industrial Heritage Renewal Based on the SEM-IPA Model: A Case Study of the Dongguan Jianyuzhou Park
Authors: Huang Wenwei , Xiong Gui , Zhong Lei , Li Keqing , Li Hongyang , Skitmore Martin , Talebian Nima
Published: March 2022

The objective of this study was to establish a satisfaction evaluation model of industrial heritage renewal by evaluating the performance of old industrial buildings after renewal, then judging the adaptability and accuracy of the transformation strategy and putting forward targeted improvement suggestions. The data were collected through a questionnaire survey and analyzed by the statistical package for social science (SPSS) software using a structural equation model (SEM) and importance–performance analysis (IPA). The results show that (1) history, culture, and placeness have the largest average satisfaction and a positive and significant impact on overall satisfaction; (2) the building regeneration effect has a significant impact on overall satisfaction; and (3) social influence, external environmental perception, and economic benefits have no significant effect on overall satisfaction, indicating the need to strengthen public participation for further research into environmental creation, market-based operation, and land conservation. This case study reflects the distinctive features of internationalization and localization, and provides an indication of local practice in China for world urban renewal and architectural heritage theories.