bluuebunny/crossref_metadata_2025 huggingface dataset:
Absent in Melanoma 2 (AIM2) Regulates the Stability of Regulatory T Cells
Authors: Lozano-Ruiz Beatriz , Tzoumpa Amalia , Martínez-Cardona Claudia , Moreno David , Aransay Ana M. , Cortazar Ana R. , Picó Joanna , Peiró Gloria , Lozano Juanjo , Zapater Pedro , Francés Rubén , González-Navajas José M.
Published: February 2022

Absent in melanoma 2 (AIM2) is a cytosolic dsDNA sensor that has been broadly studied for its role in inflammasome assembly. However, little is known about the function of AIM2 in adaptive immune cells. The purpose of this study was to investigate whether AIM2 has a cell-intrinsic role in CD4+ T cell differentiation or function. We found that AIM2 is expressed in both human and mouse CD4+ T cells and that its expression is affected by T cell receptor (TCR) activation. Naïve CD4+ T cells from AIM2-deficient (Aim2−/−) mice showed higher ability to maintain forkhead box P3 (FOXP3) expression in vitro, while their capacity to differentiate into T helper (Th)1, Th2 or Th17 cells remained unaltered. Transcriptional profiling by RNA sequencing showed that AIM2 might affect regulatory T cell (Treg) stability not by controlling the expression of Treg signature genes, but through the regulation of the cell’s metabolism. In addition, in a T cell transfer model of colitis, Aim2−/−-naïve T cells induced less severe body weight loss and displayed a higher ability to differentiate into FOXP3+ cells in vivo. In conclusion, we show that AIM2 function is not confined to innate immune cells but is also important in CD4+ T cells. Our data identify AIM2 as a regulator of FOXP3+ Treg cell differentiation and as a potential intervention target for restoring T cell homeostasis.
Molecular and Cellular Mechanisms of M. tuberculosis and SARS-CoV-2 Infections—Unexpected Similarities of Pathogenesis and What to Expect from Co-Infection
Authors: Starshinova Anna A. , Kudryavtsev Igor , Malkova Anna , Zinchenko Ulia , Karev Vadim , Kudlay Dmitry , Glushkova Angela , Starshinova Anastasiya Y. , Dominguez Jose , Villar-Hernández Raquel , Dovgalyk Irina , Yablonskiy Piotr
Published: February 2022

Tuberculosis is still an important medical and social problem. In recent years, great strides have been made in the fight against M. tuberculosis, especially in the Russian Federation. However, the emergence of a new coronavirus infection (COVID-19) has led to the long-term isolation of the population on the one hand and to the relevance of using personal protective equipment on the other. Our knowledge regarding SARS-CoV-2-induced inflammation and tissue destruction is rapidly expanding, while our understanding of the pathology of human pulmonary tuberculosis gained through more the 100 years of research is still limited. This paper reviews the main molecular and cellular differences and similarities caused by M. tuberculosis and SARS-CoV-2 infections, as well as their critical immunological and pathomorphological features. Immune suppression caused by the SARS-CoV-2 virus may result in certain difficulties in the diagnosis and treatment of tuberculosis. Furthermore, long-term lymphopenia, hyperinflammation, lung tissue injury and imbalance in CD4+ T cell subsets associated with COVID-19 could propagate M. tuberculosis infection and disease progression.
Potential Risk of Pollen from Genetically Modified MON 810 Maize Containing Cry1Ab Toxin to Protected Lepidopteran Larvae in the Pannonian Biogeographical Region—A Retrospective View
Authors: Darvas Béla , Gyurcsó Gergő , Takács Eszter , Székács András
Published: February 2022

A credible risk analysis of maize pollen containing Cry1Ab toxin must include the assessment of (i) pollen production and its Cry1 toxin content; (ii) distribution of the pollen grains in the surroundings; (iii) pollen-catching capacity of the weeds on field edges; (iv) the lifestyle of protected lepidopteran larvae living on weeds; (v) Cry1 toxin sensitivity of non-target caterpillars; and (vi) Cry1 toxin resistance of individual non-target populations. The concentration range of 5–4300 ng Cry1Ab toxin/g dry pollen determined in MON 810 pollen batches is too diverse for handling it as a single set in any mathematical modeling. Within the work carried out mainly with the DK-440 BTY cultivar, the seed samples officially received from the variety owner produced significantly different (250–470 vs. 5–15 ng/g) Cry1Ab toxin concentrations in the pollen. Nymphalis io L1-L3 larvae were nearly six times more sensitive for Dipel than Nymphalis c-album. Feeding on the back side and in a leaf nest, Vanessa atalanta may be subject to lower pollen exposures. N. io larvae may actively attempt to avoid patches with high pollen contamination. Cry1Ab toxin resistance also partially emerged in N. io populations reared in the Pannonian Biogeographical Region (Hungary).
Pathologic Complete Response in Urothelial Carcinoma Patients Receiving Neoadjuvant Immune Checkpoint Inhibitors: A Meta-Analysis
Authors: Rizzo Alessandro , Mollica Veronica , Santoni Matteo , Palmiotti Gennaro , Massari Francesco
Published: February 2022

Background. Immune checkpoint inhibitors (ICIs) have been evaluated as neoadjuvant treatment in urothelial carcinoma (UC) patients, with these agents reporting encouraging pathologic complete response (pCR) rates. Herein, we performed a systematic review and meta-analysis aimed at evaluating the incidence of pCR in UC patients treated with neoadjuvant ICI. Moreover, we investigated the impact of PD-L1 expression in this patient population, exploring the possible role of PD-L1 status as predictive biomarker. Materials and Methods. We retrieved all the relevant trials through PubMed/Medline, Cochrane Library and EMBASE; moreover, proceedings of the main international oncological meetings were also searched for relevant abstracts. Eligible trials assessed pre-operative ICI in UC patients. Results. Our meta-analysis has highlighted a pooled pCR rate of 36.6% in the overall population; interestingly, pCR was higher in PD-L1 positive compared with PD-L1 negative UCs (49.5% versus 35.1%, respectively). Conclusions. Positive signals emanating from neoadjuvant immunotherapy should encourage the scientific community to persist in the long road toward finding more effective treatments for UC patients.
Description of a Cohort of Type 1 Diabetes Patients: Analysis of Comorbidities, Prevalence of Complications and Risk of Hypoglycemia
Authors: Martínez-Ortega Antonio J. , Muñoz-Gómez Cristina , Gros-Herguido Noelia , Remón-Ruiz Pablo Jesús , Acosta-Delgado Domingo , Losada-Viñau Fernando , Pumar-López Alfonso , Mangas-Cruz Miguel Ángel , González-Navarro Irene , López-Gallardo Gema , Bellido Virginia , Soto-Moreno Alfonso Manuel
Published: February 2022

Background: Despite major medical advances, Type 1 Diabetes (T1D) patients still have greater morbimortality than the general population. Our aim was to describe our cohort of T1D patients and identify potential risk factors susceptible to prevention strategies. Methods: Cross-sectional, observational study, including T1D patients treated at our center, from 1 March 2017 to 31 March 2020. Inclusion criteria: T1D, age > 14 years and signed informed consent. Exclusion criteria: diabetes other than T1D, age < 14 years and/or refusal to participate. Results: Study population n = 2181 (49.8% females, median age at enrollment 41 years, median HbA1c 7.7%; 38.24% had at least one comorbidity). Roughly 7.45% had severe hypoglycemia (SH) within the prior year. Macro/microvascular complications were present in 42.09% (5.83% and 41.14%, respectively). The most frequent microvascular complication was diabetic retinopathy (38.02%), and coronary disease (3.21%) was the most frequent macrovascular complication. The risk of complications was higher in males than in females, mainly macrovascular. Patients with SH had a higher risk of complications (OR 1.42; 1.43 in males versus 1.42 in females). Conclusions: Our T1D population is similar to other T1D populations. We should minimize the risk of SH, and male patients should perhaps be treated more aggressively regarding cardiovascular risk factors.
Effect of Cervical Transcutaneous Spinal Cord Stimulation on Sensorimotor Cortical Activity during Upper-Limb Movements in Healthy Individuals
Authors: McGeady Ciarán , Alam Monzurul , Zheng Yong-Ping , Vučković Aleksandra
Published: February 2022

Transcutaneous spinal cord stimulation (tSCS) can improve upper-limb motor function after spinal cord injury. A number of studies have attempted to deduce the corticospinal mechanisms which are modulated following tSCS, with many relying on transcranial magnetic stimulation to provide measures of corticospinal excitability. Other metrics, such as cortical oscillations, may provide an alternative and complementary perspective on the physiological effect of tSCS. Hence, the present study recorded EEG from 30 healthy volunteers to investigate if and how cortical oscillatory dynamics are altered by 10 min of continuous cervical tSCS. Participants performed repetitive upper-limb movements and resting-state tasks while tSCS was delivered to the posterior side of the neck as EEG was recorded simultaneously. The intensity of tSCS was tailored to each participant based on their maximum tolerance (mean: 50 ± 20 mA). A control session was conducted without tSCS. Changes to sensorimotor cortical activity during movement were quantified in terms of event-related (de)synchronisation (ERD/ERS). Our analysis revealed that, on a group level, there was no consistency in terms of the direction of ERD modulation during tSCS, nor was there a dose-effect between tSCS and ERD/ERS. Resting-state oscillatory power was compared before and after tSCS but no statistically significant difference was found in terms of alpha peak frequency or alpha power. However, participants who received the highest stimulation intensities had significantly weakened ERD/ERS (10% ERS) compared to when tSCS was not applied (25% ERD; p = 0.016), suggestive of cortical inhibition. Overall, our results demonstrated that a single 10 min session of tSCS delivered to the cervical region of the spine was not sufficient to induce consistent changes in sensorimotor cortical activity among the entire cohort. However, under high intensities there may be an inhibitory effect at the cortical level. Future work should investigate, with a larger sample size, the effect of session duration and tSCS intensity on cortical oscillations.
The Efficacy of Mebeverine in the Treatment of Irritable Bowel Syndrome—A Systematic Review
Authors: Daniluk Jaroslaw , Malecka-Wojciesko Ewa , Skrzydlo-Radomanska Barbara , Rydzewska Grazyna
Published: February 2022

Background: Irritable bowel syndrome (IBS) is a common gastrointestinal tract disorder, affecting 10–20% of adults worldwide. Mebeverine is an antispasmodic agent indicated for the symptomatic treatment of abdominal pain caused by intestinal smooth muscle spasms and intestinal functional disorders in the course of IBS. The aim of this article was to perform a systematic literature review and update previous overviews of the efficacy and safety of mebeverine treatment in IBS. Methods: Major electronic medical databases, PubMed, EMBASE and Cochrane, were systematically searched from January 1965 to January 2021. Results: Twenty-two studies met our inclusion criteria, including 19 randomised trials, two observational retrospective studies, and one non-randomised, single-blinded study. Six studies reported a significant decrease in abdominal pain after mebeverine treatment (p-values ranging from <0.05 to <0.001). Only three studies showed no improvement after mebeverine treatment in terms of the severity of abdominal pain or discomfort. Some of the included studies also showed significant improvements in abnormal bowel habits, abdominal distension, as well as stool frequency and consistency. Adverse events were rare and associated mainly with IBS symptoms. Conclusions: Mebeverine is an effective treatment option in IBS, with a good safety profile and low frequency of adverse effects.
Ablation of Left Atrial Tachycardia following Catheter Ablation of Atrial Fibrillation: 12-Month Success Rates
Authors: Luik Armin , Schmidt Kerstin , Haas Annika , Unger Laura , Tzamalis Panagiotis , Brüggenjürgen Bernd
Published: February 2022

The treatment of atrial tachycardia following catheter ablation of atrial fibrillation is often challenging. Electrophysiological studies using high-resolution 3D mapping systems have contributed significantly to their understanding, and new ablation approaches have shown high rates of acute terminations with low recurrences for the clinical AT. However, patient populations are very heterogeneous, and long-term data of the freedom from any atrial tachycardia or any arrhythmia are still sparse. To evaluate long-term success, a unified patient population and predefined ablation strategies are preferred. In this study, we present 12-month success and mean 30 month follow-up data of catheter ablation of left atrial tachycardia. All 35 patients had a history of pulmonary vein isolation (PVI), 71% of which had a previous substrate modification. A total of 54 ATs, with a mean cycle length 297 ± 86 ms, 31 macro-reentries, and 4 localized reentries, were targeted. The ablation strategy to be used was given by the study protocol, depending on the type of reentry and the number of critical isthmuses. All available ablation strategies were included: standard (anatomical) lines, individual lines, critical isthmuses, and focal ablation. All ATs were terminated by ablation. A total of 91% terminated upon the first ablation strategy. Freedom from any AT after 12 months was 82%, and from any arrhythmia, it was 77%. The multi-procedure success after 30 months was 65% for any AT and 55% for any arrhythmia. In conclusion, individual ablation strategies based on the reentry mechanism and the number of critical isthmuses seems promising and demonstrates a high long-term clinical success. Tachycardia comprising a single critical isthmus can be ablated by critical isthmus ablation only. These patients present with the highest 12-month and long-term success rates.
Prediction Model of Acute Respiratory Failure in Patients with Acute Pesticide Poisoning by Intentional Ingestion: Prediction of Respiratory Failure in Pesticide Intoxication (PREP) Scores in Cohort Study
Authors: Cho Nam-Jun , Park Samel , Lyu Jiwon , Lee HwaMin , Hong Min , Lee Eun-Young , Gil Hyo-Wook
Published: February 2022

Acute respiratory failure is the primary cause of mortality in patients with acute pesticide poisoning. The aim of the present study was to develop a new and efficient score system for predicting acute respiratory failure in patients with acute pesticide poisoning. This study was a retrospective observational cohort study comprised of 679 patients with acute pesticide poisoning by intentional poisoning. We divided this population into a ratio of 3:1; training set (n = 509) and test set (n = 170) for model development and validation. Multivariable logistic regression models were used in developing a score-based prediction model. The Prediction of Respiratory failure in Pesticide intoxication (PREP) scoring system included a summation of the integer scores of the following five variables; age, pesticide category, amount of ingestion, Glasgow Coma Scale, and arterial pH. The PREP scoring system developed accurately predicted respiratory failure (AUC 0.911 [0.849−0.974], positive predictive value 0.773, accuracy 0.873 in test set). We came up with four risk categories (A, B, C and D) using PREP scores 20, 40 and 60 as the cut-off for mechanical ventilation requirement risk. The PREP scoring system developed in the present study could predict respiratory failure in patients with pesticide poisoning, which can be easily implemented in clinical situations. Further prospective studies are needed to validate the PREP scoring system.
Impact of the Type of Dialysis on Time to Transplantation: Is It Just a Matter of Immunity?
Authors: Righini Matteo , Capelli Irene , Busutti Marco , Raimondi Concettina , Comai Giorgia , Donati Gabriele , Cappuccilli Maria Laura , Ravaioli Matteo , Chieco Pasquale , La Manna Gaetano
Published: February 2022

Background: Renal transplantation represents the therapeutic gold standard in patients with end stage renal disease (ESRD). Still the role of pre-transplant dialysis in affecting time to transplantation has yet to be determined. We wanted to verify whether the type of renal replacement therapy (hemodialysis vs. peritoneal dialysis) affects time to transplantation and to identify clinical features related to the longer time to transplantation. Methods: We performed a retrospective single-center observational study on patients who had received a transplant in the Bologna Transplant Unit from 1991 to 2019, described through the analysis of digital transplant list documents for sex, age, body mass index (BMI), blood group, comorbidities, underlying disease, serology, type of dialysis, time to transplantation, Panel Reactive Antibodies (PRA) max, number of preformed anti Human Leukocyte Antigens (HLA) antibodies. A p-value < 0.05 was considered statistically significant. Results: In the 1619 patients analyzed, we observed a significant difference in time to transplant, PRA max and Preformed Antibodies Number between patients who received Hemodialysis (HD) and Peritoneal dialysis (PD). Then we performed a multiple regression analysis with all the considered factors in order to identify features that support these differences. The clinical variables that independently and directly correlate with longer time to transplantation are PRA max (p < 0.0001), Antibodies number (p < 0.0001) and HD (p < 0.0001); though AB blood group (p < 0.0001), age (p < 0.003) and PD (p < 0.0001) inversely correlate with time to transplantation. Conclusions: In our work, PD population received renal transplants in a shorter period of time compared to HD and turned out to be less immunized. Considering immunization, the type of dialysis impacts both on PRA max and on anti HLA antibodies.
C-Reactive Protein (CRP) Blocks the Desensitization of Agonistic Stimulated G Protein Coupled Receptors (GPCRs) in Neonatal Rat Cardiomyocytes
Authors: Wallukat Gerd , Mattecka Stephan , Wenzel Katrin , Schrödl Wieland , Vogt Birgit , Brunner Patrizia , Sheriff Ahmed , Kunze Rudolf
Published: February 2022

Recently, C-reactive protein (CRP) was shown to affect intracellular calcium signaling and blood pressure in vitro and in vivo, respectively. The aim of the present study was to further investigate if a direct effect on G-protein coupled receptor (GPCR) signaling by CRP can be observed by using CRP in combination with different GPCR agonists on spontaneously beating cultured neonatal rat cardiomyocytes. All used agonists (isoprenaline, clenbuterol, phenylephrine, angiotensin II and endothelin 1) affected the beat rate of cardiomyocytes significantly and after washing them out and re-stimulation the cells developed a pronounced desensitization of the corresponding receptors. CRP did not affect the basal beating-rate nor the initial increase/decrease in beat-rate triggered by different agonists. However, CRP co-incubated cells did not exhibit desensitization of the respective GPCRs after the stimulation with the different agonists. This lack of desensitization was independent of the GPCR type, but it was dependent on the CRP concentration. Therefore, CRP interferes with the desensitization of GPCRs and has to be considered as a novel regulator of adrenergic, angiotensin-1 and endothelin receptors.
A Tree-Based Architecture for High-Performance Ultra-Low-Voltage Amplifiers
Authors: Centurelli Francesco , Della Sala Riccardo , Monsurrò Pietro , Scotti Giuseppe , Trifiletti Alessandro
Published: February 2022

In this paper, we introduce a novel tree-based architecture which allows the implementation of Ultra-Low-Voltage (ULV) amplifiers. The architecture exploits a body-driven input stage to guarantee a rail-to-rail input common mode range and body-diode loading to avoid Miller compensation, thanks to the absence of high-impedance internal nodes. The tree-based structure improves the CMRR of the proposed amplifier with respect to the conventional OTA architectures and allows achievement of a reasonable CMRR even at supply voltages as low as 0.3 V and without tail current generators which cannot be used in ULV circuits. The bias currents and the static output voltages of all the stages implementing the architecture are accurately set through the gate terminals of biasing transistors in order to guarantee good robustness against PVT variations. The proposed architecture and the implementing stages are investigated from an analytical point of view and design equations for the main performance metrics are presented to provide insight into circuit behavior. A 0.3 V supply voltage, subthreshold, ultra-low-power (ULP) OTA, based on the proposed tree-based architecture, was designed in a commercial 130 nm CMOS process. Simulation results show a dc gain higher than 52 dB with a gain-bandwidth product of about 35 kHz and reasonable values of CMRR and PSRR, even at such low supply voltages and considering mismatches. The power consumption is as low as 21.89 nW and state-of-the-art small-signal and large-signal FoMs are achieved. Extensive parametric and Monte Carlo simulations show the robustness of the proposed circuit to PVT variations and mismatch. These results confirm that the proposed OTA is a good candidate to implement ULV, ULP, high performance analog building blocks for directly harvested IoT nodes.
Buckling and Post-Buckling Behavior of Perfect/Perforated Composite Cylindrical Shells under Hydrostatic Pressure
Authors: Shen Ke-Chun , Yang Zhao-Qi , Jiang Lei-Lei , Pan Guang
Published: February 2022

In this paper, the buckling and post-buckling behavior of perfect and perforated composite cylindrical shells subjected to external hydrostatic pressure was experimentally investigated. Three filament wound composite cylindrical shells were fabricated from T700-12K Carbon fiber/Epoxy, two of which were perforated and reinforced. A test platform was established that allows researchers to observe the deformation of composite cylindrical shells under hydrostatic pressure in real-time during test. According to experimental observation, strain response and buckling deformation wave were discussed. Comparative analysis was carried out based on the experimental observation and finite element prediction. Results show that the deformation of composite cylindrical shell under hydrostatic pressure included linear compression, buckling and post-buckling processes. The buckling behavior was a progressive evolution process which accounted for 20% of the load history, and strain reversal phenomenon generally occurred at the trough of the buckling wave. As for the postbuckling deformation, the load carrying capacity of the shell gradually decreased while the magnitude of strain continued increasing. Both the perfect and perforated composite cylindrical shells collapsed at the trough of the buckling wave. Comparing with the perfect shell, it was validated the reinforcement design could effectively ensure the load carrying capacity of the perforated composite cylindrical shell.
COVID-19 Vaccination Intention Associated with Behaviors towards Protection and Perceptions Regarding the Pandemic
Authors: Dafogianni Chrysoula , Mangoulia Polyxeni , Pappa Despoina , Xanthopoulou Panagiota , Koutelekos Ioannis , Zografakis-Sfakianakis Mixalis , Ferentinou Eftychia , Fountouki Antigoni , Drakopoulou Marianna , Giga Anna , Anastasiou Nikos , Margari Nikoletta , Fasoi Georgia
Published: February 2022

Background: The impressively rapid availability of different types of COVID-19 vaccines and, on the other hand, the degree of their effectiveness as opposed to the likelihood of serious or non-serious side effects place a fairly large percentage of the population at a crossroads regarding the choice to get vaccinated or not, hence threatening achievement of total immunization coverage and full immunity. This study aimed to assess COVID-19 vaccination intention in Greece regarding protection behaviors and perceptions of the pandemic. Methods: A total of 3753 participants completed a specially designed electronic questionnaire anonymously and voluntarily. The study population consisted of healthcare workers, students, members of professional societies, teachers, and professors. The questionnaire was composed of four parts pertaining to demographic data and possible changes in hygiene attitudes during the COVID-19 pandemic. Results: In total, 43.3% of the participants stated that SARS-CoV-2 poses a significant risk. The most widespread protection practice was avoiding crowded places (90.1%), followed by reducing the use of public transport (86.1%) and washing their hands with soap and water more often than usual (84.2%). Women undertook significantly more behavioral changes than men and participants of other nationalities. About half of the participants (44%) implemented seven behavioral changes. Lower personal and general perceived risk due to COVID-19 was significantly associated with lower intention to get vaccinated. Conclusion: Strong hesitancy was observed towards COVID-19 vaccination. There is a need for further studies to be conducted to investigate the benefits and safety of vaccines for the purpose of better informing the public. Healthcare personnel can and should play a key role in this process.
Prediction of Gastric Gastrointestinal Stromal Tumors before Operation: A Retrospective Analysis of Gastric Subepithelial Tumors
Authors: Lin Yu-Ning , Chen Ming-Yan , Tsai Chun-Yi , Chou Wen-Chi , Hsu Jun-Te , Yeh Chun-Nan , Yeh Ta-Sen , Liu Keng-Hao
Published: February 2022

Gastrointestinal stromal tumors (GISTs), leiomyomas, and schwannomas are the most common gastric subepithelial tumors (GSETs) with similar endoscopic findings. Preoperative prediction of GSETs is difficult. This study analyzed and predicted GSET diagnosis through a retrospective review of 395 patients who underwent surgical resection of GISTs, leiomyomas, and schwannomas measuring 2–10 cm. GSETs were divided by size (group 2–5, >2 and ≤5 cm; group 5–10, >5 and ≤10 cm) for analysis. Demographics, clinical symptoms, and images were analyzed. A recursive partitioning analysis (RPA) was used to identify optimal classifications for specific GSET diagnoses. GIST patients were relatively older than other patients. Both groups had higher proportions of UGI bleeding, lower hemoglobin (Hb) levels, and a higher ratio of necrosis on their computed tomography (CT) scans. The RPA tree showed that (a) age ≤ 55, Hb ≥ 10.7, and CT necrosis; (b) age ≤ 55 and Hb < 10.7; (c) age >55 and Hb < 12.9; and (d) age >55 and CT hetero-/homogeneity can predict high GIST risk in group 2–5. Positive or negative CT necrosis, with age >55, can predict high GIST risk in group 5–10. GIST patients were older and presented with low Hb levels and tumor necrosis. In RPA, the accuracy reached 85% and 89% in groups 2–5 and 5–10, respectively.
Personalized Nutrition in Patients with Type 2 Diabetes and Chronic Kidney Disease: The Two-Edged Sword of Dietary Protein Intake
Authors: Oosterwijk Milou M. , Navis Gerjan , Bakker Stephan J. L. , Laverman Gozewijn D.
Published: February 2022

In type 2 diabetes (T2D), there is a general and strong focus on carbohydrate restriction. However, this may have unwarranted consequences for those with concomitant chronic kidney disease (CKD) since decreasing intake of carbohydrates implies a higher proportion of dietary protein, which is of critical debate in patients with CKD due to its ambiguous implications in maintaining either kidney function or nutritional status. We evaluated adherence to the protein recommendations, taking into account the nutritional status of patients with T2D with or without CKD. Patients were divided in three groups according to their estimated Glomerular Filtration Rate (eGFR): mild to no CKD (eGFR > 60 mL/min/1.73 m2), moderate CKD (eGFR 30–60 mL/min/1.73 m2), or advanced CKD (eGFR < 30 mL/min/1.73 m2). Regarding adherence to the protein recommendations, 17% of the patients without advanced CKD consumed < 0.8 g/kg/day, 29% of the patients with moderate CKD consumed > 1.3 g/kg/day, and 60% of the patients with advanced CKD consumed > 1.0 g/kg/day. In addition, patients with moderate- or advanced CKD tend to have a lower muscle mass, normalized by height, compared to patients with mild to no CKD (p < 0.001), while body mass index was not significantly different between patients with or without CKD (p = 0.44). We found that although dietary protein restriction has not been indicated in either of the CKD stages, approximately 10% had a dietary protein intake < 0.8 g/kg/day, with accompanying risks of malnourishment and sarcopenia. Our main advice is to maintain a dietary protein intake of at least 0.8 g/kg/day in order to prevent patients from becoming malnourished and sarcopenic.
The Efficacy of Thrombin-Gelatin Matrix in Hemostasis for Large Breast Tumor after Vacuum-Assisted Breast Biopsy
Authors: Tzeng Yen-Dun Tony , Liu Shiuh-Inn , Wang Being-Whey , Chen Yu-Chia , Chang Po-Ming , Chen I-Shu , Sheu Jim Jinn-Chyuan , Hsiao Jui-Hu
Published: February 2022

Background: Vacuum-assisted breast biopsy (VABB) for benign breast tumor excision is a developing trend in breast surgery. The most common complication of VABB is hematoma. We assessed the efficiency of the thrombin-gelatin matrix (TGM) for hemostasis after VABB. Methods: From December 2013 to June 2017, 147 patients with breast tumors > 2 cm in size were treated with a 7-gauge ultrasound-guided EnCor EnSpire® breast biopsy system. After VABB, the TGM was applied using an iron-tube device. After injection, brief external compression for 15 min and postoperative bandage compression for approximately 12 h were applied. The medical records were reviewed and analyzed for hematoma and acute bleeding at 1 and 3 months after VABB. Results: A total of 72 patients received hemostasis via TGM, and 75 patients received hemostasis by compression. The rates of postoperative acute bleeding in the TGM group were significantly lower than those in the non-TGM group (5.5% vs. 22.7%, p = 0.003). Among patients with hematoma, there was no statistically significant difference between the two groups (25% vs. 26.7%, p = 0.85). Conclusions: This is the first cohort study to apply the TGM hemostatic matrix for post-VABB hemostasis. The TGM hemostatic matrix could be an option for patients with large breast tumors.
Effects of Pain Neuroscience Education Combined with Lumbar Stabilization Exercise on Strength and Pain in Patients with Chronic Low Back Pain: Randomized Controlled Trial
Authors: Kim Ki-Sang , An Jungae , Kim Ju-O , Lee Mi-Young , Lee Byoung-Hee
Published: February 2022

Chronic low back pain that lasts more than 12 weeks causes mental and physical distress. This study investigated the effects of pain neuroscience education combined with lumbar stabilization exercises on strength, pain, flexibility, and activity disorder index in female patients with chronic low back pain. Thirty-five female patients with chronic low back pain were randomly divided into two groups: the pain neuroscience education (PNE) combined with lumbar stabilization exercises (LSEs) group (n = 18, experimental group) and the lumbar stabilization exercises alone group (n = 17, control group). The experimental group underwent PNE combined with LSEs for 30 min per session, twice per week for 8 weeks, and the control group underwent LSEs only. The primary outcomes were strength (sit-up and back-up movements), Numerical Pain Rating Scale (NPRS), Korean Pain Catastrophizing Scale (K-PCS), and Tampa Scale of Kinesio-phobia-11 (TSK-11) for pain. The secondary outcomes were modified–modified Schober’s test (MMST) and finger to floor test (FFT) for flexibility and activity disorder (Roland–Morris Disability Questionnaire index). A significant difference was observed in the primary outcomes after intervention in the abdominal muscle strength (group difference, mean, −7.50; 95% CI, −9.111 to –5.889, F = 9.598; ANCOVA p = 0.005), the back muscle strength (group difference, mean, −9.722; 95% CI, −10.877 to –8.568, F = 7.102; ANCOVA p = 0.014), the NPRS (group difference, mean, 1.89; 95% CI,1.65 to 2.12, F = 24.286; ANCOVA p < 0.001), K-PCS (group difference, mean, 7.89; 95% CI, 7.02 to 8.76, F = 11.558; ANCOVA p = 0.003), and TSK-11 (group difference, mean, 16.79; 95% CI, 13.99 to 19.59, F = 13.179; ANCOVA p = 0.014) for pain. In the secondary outcomes, there was a significant difference in the FFT (group difference, mean, −0.66; 95%CI, −0.99 to −0.33, F = 4.327; ANCOVA p = 0.049), whereas the difference in flexibility (MMST) and activity disorder index of the secondary outcomes did not reach significance. Therefore, this study confirmed that PNE combined with LSEs is an effective intervention compared to LSE alone in improving muscle strength and pain in female patients with chronic low back pain.
Strategies to Mitigate the Deteriorating Habitat Quality in Dong Trieu District, Vietnam
Authors: Vu Thi Thu , Shen Yuan , Lai Hung-Yu
Published: February 2022

Dong Trieu district is a vital connection for territorial ecological security and human welfare between Hanoi (the capital of Vietnam) and Quang Ninh province. Therefore, habitat quality (HQ) is of extraordinary importance to the area’s sustainable development. The ArcGIS platform, Dyna-CLUE, and InVEST models were utilized in this study to assess the spatial and temporal transformations of land use and the changes of HQ in 2030 under various scenarios, with intentions to find strategies that may mitigate the HQ’s deteriorating trend in the district. Simulated results indicated that, assuming the development is maintained as usual, the average HQ of the District at 2030 could diminish by 0.044 from that of 2019 (a four-times decrease compared to the previous decade). Cases comprised of four basic scenarios, including development as usual, built-up expansion slowdown, forest protection emphasized, and agricultural land conversion, were used to identify potential strategies to mitigate the deteriorating trend. Simulated results revealed that keeping the built-up expansion rate lower than 100 ha y−1, the deforestation rate lower than 20 ha y−1, and preferring orchards over agricultural land conversion is required to limit the drop in HQ to within 0.01 in the next decade. Other than the existing population growth control policy, new guidelines such as (1) changing urban expansion type from outward to upward to control the built-up expansion rate, (2) substituting forest-harming industries to forest-preservation industries to reduce deforestation rate, (3) encouraging orchards preferred over agricultural land conversion to increase incomes while maintaining higher habitat quality, (4) practicing better farming technologies to improve crop production and to alleviate potential food security issues due to considerable reduction in cropland, and (5) promoting Green Infrastructure and the Belt and Road Initiative to increase urban green cover and raise residents’ income should be considered in designing the new mitigation strategies.
Impact of Local Grasslands on Wild Grass Pollen Emission in Bavaria, Germany
Authors: Jung Stephan , Yuan Ye , Stange Del Carpio Maria , Pawlik Thomas , Hartmann Stephan , Estrella Nicole , Oteros Jose , Traidl-Hoffmann Claudia , Damialis Athanasios , Buters Jeroen , Menzel Annette
Published: February 2022

Meteorological conditions and the distribution of pollen sources are the two most decisive factors influencing the concentration of airborne grass pollen. However, knowledge about land-use types, their potential pollen emission, and the importance of local sources remains limited. In this study, wild grass pollen concentrations from 27 stations in Bavaria, Germany, were linked to potential pollen within a 30 km radius. Agricultural grass pollen sources were derived from the InVeKos database, which contains detailed information on agricultural land-use types and their spatial distribution. Non-agricultural grassland was identified by OpenStreetMap. Further source classification was conducted using a cultivation intensity indicator and wind direction. We show that the grassland percentage and pollen concentrations, specified as annual pollen integral and pollen peak vary strongly between pollen stations. Correlation analyses indicated that the impact of the grassland on pollen concentration was greater within 10 km of the pollen traps. At greater distances, the correlation coefficient between the grassland percentage and pollen indicators steadily declined.
Comparing Teacher Priorities and Student Uptake in EMI Lectures: An Exploratory Study
Authors: Siegel Joseph
Published: February 2022

English medium instruction (EMI) has been increasing in higher education with broad intentions of stimulating internationalization and cross-cultural learning experiences. This form of education presents opportunities and challenges for teachers and students alike. Key challenges involve various levels of second language (L2) speaking and listening abilities among teachers and students operating in EMI contexts. This exploratory study therefore examines the relationship between the main ideas two EMI lecturers in Sweden intended for their students to learn during lectures and the main ideas that EMI students report learning in the same lectures. Prior to six lectures, the teachers summarized to the researcher the main ideas to be included in the respective lecture. Immediately following the lecture, students provided their own summaries of the main ideas. A keyword analysis comparing the teachers’ intended messages and students’ reports shows that students may not be recognizing and acquiring the main ideas that the teacher intends. Further analysis distinguished two sub-groups of students: those with self-reported Swedish as a first language (L1) and those with self-reported L1s other than Swedish. A binomial proportion test showed that L1 impacted the amount of lecture main idea key words reported by the students in this study. The paper closes with a pedagogic perspective encouraging EMI lecturers to monitor student uptake on a regular basis and adjust their lecture delivery to support better learning and retention of content delivered via EMI.
Potassium at the Origins of Life: Did Biology Emerge from Biotite in Micaceous Clay?
Authors: Hansma Helen Greenwood
Published: February 2022

Intracellular potassium concentrations, [K+], are high in all types of living cells, but the origins of this K+ are unknown. The simplest hypothesis is that life emerged in an environment that was high in K+. One such environment is the spaces between the sheets of the clay mineral mica. The best mica for life’s origins is the black mica, biotite, because it has a high content of Mg++ and because it has iron in various oxidation states. Life also has many of the characteristics of the environment between mica sheets, giving further support for the possibility that mica was the substrate on and within which life emerged. Here, a scenario for life’s origins is presented, in which the necessary processes and components for life arise in niches between mica sheets; vesicle membranes encapsulate these processes and components; the resulting vesicles fuse, forming protocells; and eventually, all of the necessary components and processes are encapsulated within individual cells, some of which survive to seed the early Earth with life. This paper presents three new foci for the hypothesis of life’s origins between mica sheets: (1) that potassium is essential for life’s origins on Earth; (2) that biotite mica has advantages over muscovite mica; and (3) that micaceous clay is a better environment than isolated mica for life’s origins.
The p53-Driven Anticancer Effect of Ribes fasciculatum Extract on AGS Gastric Cancer Cells
Authors: Kim Myeong-Jin , Kawk Hye-Won , Kim Sang-Hyeon , Lee Hyo-Jae , Seo Ji-Won , Lee Chang-Yeol , Kim Young-Min
Published: February 2022

Cancer metastasis is directly related to the survival rate of cancer patients. Although cancer metastasis proceeds by the movement of cancer cells, it is fundamentally caused by its resistance to anoikis, a mechanism of apoptosis caused by the loss of adhesion of cancer cells. Therefore, it was found that inhibiting cancer migration and reducing anoikis resistance are important for cancer suppression, and natural compounds can effectively control it. Among them, Ribes fasciculatum, which has been used as a medicinal plant, was confirmed to have anticancer potential, and experiments were conducted to prove various anticancer effects by extracting Ribes fasciculatum (RFE). Through various experiments, it was observed that RFE induces apoptosis of AGS gastric cancer cells, arrests the cell cycle, induces oxidative stress, and reduces mobility. It was also demonstrated that anoikis resistance was attenuated through the downregulation of proteins, such as epidermal growth factor receptor (EGFR). Moreover, the anticancer effect of RFE depends upon the increase in p53 expression, suggesting that RFE is suitable for the development of p53-targeted anticancer materials. Moreover, through xenotransplantation, it was found that the anticancer effect of RFE confirmed in vitro was continued in vivo.
The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity
Authors: Timofeeva Anna , Sedykh Sergey , Maksimenko Lada , Sedykh Tatyana , Skudarnov Sergey , Ostapova Tatyana , Yaschenko Svetlana , Gashnikova Natalya , Nevinsky Georgy
Published: February 2022

Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific κλ-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.
Research on Hot Corrosion Behavior of DZ40M and K452 Superalloys in NaCl Molten Salt
Authors: Wan Lei , Zhang Zeyu , Wang Wenquan , Xue Yunpeng , Shen Jubao , Sun Tao , Sun Haiou
Published: February 2022

The corrosion of cobalt-based DZ40M and nickel-based K452 superalloy at 900 °C was investigated by NaCl salt coating. Accordingly, the differences in hot corrosion behavior were analyzed considering the development methods and elementary composition by comparing the two alloys’ failure. Then, the corrosion mechanism induced by NaCl was proposed by comparing oxidation and hot corrosion behavior. The relatively continuous Al2O3 and TiO2 formed on K452 superalloy with higher content of Al and Ti have lower solubility and less damage in Na2O. Thus, the hot corrosion rate of K452 is lower than that of DZ40M with higher content of C, Cr, and W.
Study of Impact Characteristics of ZrO2 Ceramic Composite Projectiles on Ceramic Composite Armor
Authors: Wang Weizhan , Zhao Taiyong , Meng Fangao , Tian Peng , Li Guanglei , Chen Zhigang
Published: February 2022

Exploring new armor-piercing materials is crucial for improving the penetrative ability of projectiles. Based on the process of in situ solidification injection molding through ceramic dispersant hydrolytic degradation, a ZrO2 ceramic material suitable for use as the tip of a 12.7 mm kinetic energy (KE) projectile was prepared. The ZrO2 ceramic tip can be matched with the metal core of a conventional projectile to form a ceramic composite projectile, increasing the damage to the Al2O3 ceramic composite armor. Specifically, the ZrO2 ceramic tip can increase the impact load on the Al2O3 ceramic panel, prolonging the pre-damage phase and reducing the stable penetration phase, shortening the mass erosion time of the metal core compared with a 12.7 mm metal KE projectile tip. The ceramic composite projectile with the ZrO2 ceramic tip has a lower critical penetration velocity than a 12.7 mm metal KE projectile for Al2O3 ceramic composite armor. Furthermore, the residual velocity, residual length, and residual mass of the metal core of the ceramic composite projectile that penetrated the Al2O3 ceramic composite armor are greater than those of a 12.7 mm metal KE projectile.
A Review of Key Technologies for High-Speed Motorized Spindles of CNC Machine Tools
Authors: Dai Ye , Tao Xueshi , Li Zhaolong , Zhan Shiqiang , Li Yang , Gao Yanhua
Published: February 2022

The high-speed and high-precision motorized spindle is the future development trend of the CNC machine tool field, and has become the focus of research in the world. High-speed motorized spindles tend to develop in the direction of high precision, high speed, low energy consumption, high efficiency, and high reliability. We undertake a through, systematic review of the development history perspective of the research on precision bearing technology, dynamic balancing technology, thermal error measurement and compensation technology with regard to the key technologies of high-speed motorized spindles. On this basis, the current level of development of key technologies for high-speed motorized spindles is analyzed, and the objective advantages and disadvantages of existing technologies are summarized. Finally, the development tendency of high-speed motorized spindle technology is predicted and foreseen.
Predictive Ability of Machine-Learning Methods for Vitamin D Deficiency Prediction by Anthropometric Parameters
Authors: Patino-Alonso Carmen , Gómez-Sánchez Marta , Gómez-Sánchez Leticia , Sánchez Salgado Benigna , Rodríguez-Sánchez Emiliano , García-Ortiz Luis , Gómez-Marcos Manuel A.
Published: February 2022

Background: Vitamin D deficiency affects the general population and is very common among elderly Europeans. This study compared different supervised learning algorithms in a cohort of Spanish individuals aged 35–75 years to predict which anthropometric parameter was most strongly associated with vitamin D deficiency. Methods: A total of 501 participants were recruited by simple random sampling with replacement (reference population: 43,946). The analyzed anthropometric parameters were waist circumference (WC), body mass index (BMI), waist-to-height ratio (WHtR), body roundness index (BRI), visceral adiposity index (VAI), and the Clinical University of Navarra body adiposity estimator (CUN-BAE) for body fat percentage. Results: All the anthropometric indices were associated, in males, with vitamin D deficiency (p < 0.01 for the entire sample) after controlling for possible confounding factors, except for CUN-BAE, which was the only parameter that showed a correlation in females. Conclusions: The capacity of anthropometric parameters to predict vitamin D deficiency differed according to sex; thus, WC, BMI, WHtR, VAI, and BRI were most useful for prediction in males, while CUN-BAE was more useful in females. The naïve Bayes approach for machine learning showed the best area under the curve with WC, BMI, WHtR, and BRI, while the logistic regression model did so in VAI and CUN-BAE.