bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Zinc Efficiency-Associated Loci (ZEALs) and Candidate Genes for Zn Deficiency Tolerance of Two Recombination Inbred Line Populations in Maize
Authors: Xu Jianqin , Qin Xiaoxin , Ni Zhongfu , Chen Fanjun , Fu Xiuyi , Yu Futong
Published: April 2022

Zinc (Zn) deficiency is one of the most common micronutrient disorders in cereal plants, greatly impairing crop productivity and nutritional quality. Identifying the genes associated with Zn deficiency tolerance is the basis for understanding the genetic mechanism conferring tolerance. In this study, the K22×BY815 and DAN340×K22 recombination inbred line (RIL) populations, which were derived from Zn-inefficient and Zn-efficient inbred lines, were utilized to detect the quantitative trait loci (QTLs) associated with Zn deficiency tolerance and to further identify candidate genes within these loci. The BLUP (Best Linear Unbiased Prediction) values under Zn-deficient condition (-Zn) and the ratios of the BLUP values under Zn deficient condition to the BLUP values under Zn-sufficient condition (-Zn/CK) were used to perform linkage mapping. In QTL analysis, 21 QTLs and 33 QTLs controlling the Zn score, plant height, shoot and root dry weight, and root-to-shoot ratio were detected in the K22×BY815 population and the DAN340×K22 population, explaining 5.5–16.6% and 4.2–23.3% of phenotypic variation, respectively. In addition, seventeen candidate genes associated with the mechanisms underlying Zn deficiency tolerance were identified in QTL colocalizations or the single loci, including the genes involved in the uptake, transport, and redistribution of Zn (ZmIRT1, ZmHMAs, ZmNRAMP6, ZmVIT, ZmNAS3, ZmDMAS1, ZmTOM3), and the genes participating in the auxin and ethylene signal pathways (ZmAFBs, ZmIAA17, ZmETR, ZmEIN2, ZmEIN3, ZmCTR3, ZmEBF1). Our findings will broaden the understanding of the genetic structure of the tolerance to Zn deficiency in maize.
Exosomal miRNAs as a Promising Source of Biomarkers in Colorectal Cancer Progression
Authors: Bakhsh Tahani , Alhazmi Safiah , Alburae Najla Ali , Farsi Ali , Alzahrani Faisal , Choudhry Hani , Bahieldin Ahmed
Published: April 2022

Colorectal cancer (CRC) is the third most common type of cancer worldwide amongst males and females. CRC treatment is multidisciplinary, often including surgery, chemotherapy, and radiotherapy. Early diagnosis of CRC can lead to treatment initiation at an earlier stage. Blood biomarkers are currently used to detect CRC, but because of their low sensitivity and specificity, they are considered inadequate diagnostic tools and are used mainly for following up patients for recurrence. It is necessary to detect novel, noninvasive, specific, and sensitive biomarkers for the screening and diagnosis of CRC at earlier stages. The tumor microenvironment (TME) has an essential role in tumorigenesis; for example, extracellular vesicles (EVs) such as exosomes can play a crucial role in communication between cancer cells and different components of TME, thereby inducing tumor progression. The importance of miRNAs that are sorted into exosomes has recently attracted scientists’ attention. Some unique sequences of miRNAs are favorably packaged into exosomes, and it has been illustrated that particular miRNAs can be directed into exosomes by special mechanisms that occur inside the cells. This review illustrates and discusses the sorted and transported exosomal miRNAs in the CRC microenvironment and their impact on CRC progression as well as their potential use as biomarkers.
The Genomic Landscape of Corticotroph Tumors: From Silent Adenomas to ACTH-Secreting Carcinomas
Authors: Andonegui-Elguera Sergio , Silva-Román Gloria , Peña-Martínez Eduardo , Taniguchi-Ponciano Keiko , Vela-Patiño Sandra , Remba-Shapiro Ilan , Gómez-Apo Erick , Espinosa-de-los-Monteros Ana-Laura , Portocarrero-Ortiz Lesly A. , Guinto Gerardo , Moreno-Jimenez Sergio , Chavez-Macias Laura , Saucedo Renata , Basurto-Acevedo Lourdes , Lopez-Felix Blas , Gonzalez-Torres Carolina , Gaytan-Cervantes Javier , Ayala-Sumuano Jorge T. , Burak-Leipuner Andres , Marrero-Rodríguez Daniel , Mercado Moisés
Published: April 2022

Corticotroph cells give rise to aggressive and rare pituitary neoplasms comprising ACTH-producing adenomas resulting in Cushing disease (CD), clinically silent ACTH adenomas (SCA), Crooke cell adenomas (CCA) and ACTH-producing carcinomas (CA). The molecular pathogenesis of these tumors is still poorly understood. To better understand the genomic landscape of all the lesions of the corticotroph lineage, we sequenced the whole exome of three SCA, one CCA, four ACTH-secreting PA causing CD, one corticotrophinoma occurring in a CD patient who developed Nelson syndrome after adrenalectomy and one patient with an ACTH-producing CA. The ACTH-producing CA was the lesion with the highest number of single nucleotide variants (SNV) in genes such as USP8, TP53, AURKA, EGFR, HSD3B1 and CDKN1A. The USP8 variant was found only in the ACTH-CA and in the corticotrophinoma occurring in a patient with Nelson syndrome. In CCA, SNV in TP53, EGFR, HSD3B1 and CDKN1A SNV were present. HSD3B1 and CDKN1A SNVs were present in all three SCA, whereas in two of these tumors SNV in TP53, AURKA and EGFR were found. None of the analyzed tumors showed SNV in USP48, BRAF, BRG1 or CABLES1. The amplification of 17q12 was found in all tumors, except for the ACTH-producing carcinoma. The four clinically functioning ACTH adenomas and the ACTH-CA shared the amplification of 10q11.22 and showed more copy-number variation (CNV) gains and single-nucleotide variations than the nonfunctioning tumors.
The Impact of Mouse Preterm Birth Induction by RU-486 on Microglial Activation and Subsequent Hypomyelination
Authors: Morin Cécile , Guenoun David , Sautet Irvin , Faivre Valérie , Csaba Zsolt , Schwendimann Leslie , Young-Ten Pierrette , Van Steenwinckel Juliette , Gressens Pierre , Bokobza Cindy
Published: April 2022

Preterm birth (PTB) represents 15 million births every year worldwide and is frequently associated with maternal/fetal infections and inflammation, inducing neuroinflammation. This neuroinflammation is mediated by microglial cells, which are brain-resident macrophages that release cytotoxic molecules that block oligodendrocyte differentiation, leading to hypomyelination. Some preterm survivors can face lifetime motor and/or cognitive disabilities linked to periventricular white matter injuries (PWMIs). There is currently no recommendation concerning the mode of delivery in the case of PTB and its impact on brain development. Many animal models of induced-PTB based on LPS injections exist, but with a low survival rate. There is a lack of information regarding clinically used pharmacological substances to induce PTB and their consequences on brain development. Mifepristone (RU-486) is a drug used clinically to induce preterm labor. This study aims to elaborate and characterize a new model of induced-PTB and PWMIs by the gestational injection of RU-486 and the perinatal injection of pups with IL-1beta. A RU-486 single subcutaneous (s.c.) injection at embryonic day (E)18.5 induced PTB at E19.5 in pregnant OF1 mice. All pups were born alive and were adopted directly after birth. IL-1beta was injected intraperitoneally from postnatal day (P)1 to P5. Animals exposed to both RU-486 and IL-1beta demonstrated microglial reactivity and subsequent PWMIs. In conclusion, the s.c. administration of RU-486 induced labor within 24 h with a high survival rate for pups. In the context of perinatal inflammation, RU-486 labor induction significantly decreases microglial reactivity in vivo but did not prevent subsequent PWMIs.
Galaxy Dnpatterntools for Computational Analysis of Nucleosome Positioning Sequence Patterns
Authors: Pranckeviciene Erinija , Hosid Sergey , Maziukas Indiras , Ioshikhes Ilya
Published: April 2022

Nucleosomes are basic units of DNA packing in eukaryotes. Their structure is well conserved from yeast to human and consists of the histone octamer core and 147 bp DNA wrapped around it. Nucleosomes are bound to a majority of the eukaryotic genomic DNA, including its regulatory regions. Hence, they also play a major role in gene regulation. For the latter, their precise positioning on DNA is essential. In the present paper, we describe Galaxy dnpatterntools—software package for nucleosome DNA sequence analysis and mapping. This software will be useful for computational biologists practitioners to conduct more profound studies of gene regulatory mechanisms.
Comparison of BMIPP-SPECT/CT to 18FDG-PET/CT for Imaging Brown or Browning Fat in a Preclinical Model
Authors: Frankl Joseph A. , An Yu , Sherwood Amber , Hao Guiyang , Huang Feng-Yun , Thapa Pawan , Clegg Deborah J. , Sun Xiankai , Scherer Philipp E. , Öz Orhan K.
Published: April 2022

Obesity is a leading cause of preventable death and morbidity. To elucidate the mechanisms connecting metabolically active brown adipose tissue (BAT) and metabolic health may provide insights into methods of treatment for obesity-related conditions. 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18FDG-PET/CT) is traditionally used to image human BAT activity. However, the primary energy source of BAT is derived from intracellular fatty acids and not glucose. Beta-methyl-p-iodophenylpentadecanoic acid (BMIPP) is a fatty acid analogue amenable to in vivo imaging by single photon emission computed tomography/CT (SPECT/CT) when radiolabeled with iodine isotopes. In this study, we compare the use of 18FDG-PET/CT and 125I-BMIPP-SPECT/CT for fat imaging to ascertain whether BMIPP is a more robust candidate for the non-invasive evaluation of metabolically active adipose depots. Interscapular BAT, inguinal white adipose tissue (iWAT), and gonadal white adipose tissue (gWAT) uptake of 18FDG and 125I-BMIPP was quantified in mice following treatment with the BAT-stimulating drug CL-316,243 or saline vehicle control. After CL-316,243 treatment, uptake of both radiotracers increased in BAT and iWAT. The standard uptake value (SUVmean) for 18FDG and 125I-BMIPP significantly correlated in these depots, although uptake of 125I-BMIPP in BAT and iWAT more closely mimicked the fold-change in metabolic rate as measured by an extracellular flux analyzer. Herein, we find that imaging BAT with the radioiodinated fatty acid analogue BMIPP yields more physiologically relevant data than 18FDG-PET/CT, and its conventional use may be a pivotal tool for evaluating BAT in both mice and humans.
NAD+ Precursors Repair Mitochondrial Function in Diabetes and Prevent Experimental Diabetic Neuropathy
Authors: Chandrasekaran Krish , Najimi Neda , Sagi Avinash R. , Yarlagadda Sushuma , Salimian Mohammad , Arvas Muhammed Ikbal , Hedayat Ahmad F. , Kevas Yanni , Kadakia Anand , Russell James W.
Published: April 2022

Axon degeneration in diabetic peripheral neuropathy (DPN) is associated with impaired NAD+ metabolism. We tested whether the administration of NAD+ precursors, nicotinamide mononucleotide (NMN) or nicotinamide riboside (NR), prevents DPN in models of Type 1 and Type 2 diabetes. NMN was administered to streptozotocin (STZ)-induced diabetic rats and STZ-induced diabetic mice by intraperitoneal injection at 50 or 100 mg/kg on alternate days for 2 months. mice The were fed with a high fat diet (HFD) for 2 months with or without added NR at 150 or 300 mg/kg for 2 months. The administration of NMN to STZ-induced diabetic rats or mice or dietary addition of NR to HFD-fed mice improved sensory function, normalized sciatic and tail nerve conduction velocities, and prevented loss of intraepidermal nerve fibers in skin samples from the hind-paw. In adult dorsal root ganglion (DRG) neurons isolated from HFD-fed mice, there was a decrease in NAD+ levels and mitochondrial maximum reserve capacity. These impairments were normalized in isolated DRG neurons from NR-treated mice. The results indicate that the correction of NAD+ depletion in DRG may be sufficient to prevent DPN but does not significantly affect glucose tolerance, insulin levels, or insulin resistance.
Calprotectin and Imbalances between Acute-Phase Mediators Are Associated with Critical Illness in COVID-19
Authors: Kassianidis Georgios , Siampanos Athanasios , Poulakou Garyphalia , Adamis George , Rapti Aggeliki , Milionis Haralampos , Dalekos George N. , Petrakis Vasileios , Sympardi Styliani , Metallidis Symeon , Alexiou Zoi , Gkavogianni Theologia , Giamarellos-Bourboulis Evangelos J. , Theoharides Theoharis C.
Published: April 2022

The trajectory from moderate and severe COVID-19 into acute respiratory distress syndrome (ARDS) necessitating mechanical ventilation (MV) is a field of active research. We determined serum levels within 24 h of presentation of 20 different sets of mediators (calprotectin, pro- and anti-inflammatory cytokines, interferons) of patients with COVID-19 at different stages of severity (asymptomatic, moderate, severe and ARDS/MV). The primary endpoint was to define associations with critical illness, and the secondary endpoint was to identify the pathways associated with mortality. Results were validated in serial measurements of mediators among participants of the SAVE-MORE trial. Levels of the proinflammatory interleukin (IL)-8, IL-18, matrix metalloproteinase-9, platelet-derived growth factor (PDGF)-B and calprotectin (S100A8/A9) were significantly higher in patients with ARDS and MV. Levels of the anti-inflammatory IL-1ra and IL-33r were also increased; IL-38 was increased only in asymptomatic patients but significantly decreased in the more severe cases. Multivariate ordinal regression showed that pathways of IL-6, IL-33 and calprotectin were associated with significant probability for worse outcome. Calprotectin was serially increased from baseline among patients who progressed to ARDS and MV. Further research is needed to decipher the significance of these findings compared to other acute-phase reactants, such as C-reactive protein (CRP) or ferritin, for the prognosis and development of effective treatments.
Annoying Psoriasis and Atopic Dermatitis: A Narrative Review
Authors: Chen Wei-Yu , Chen Shao-Chuan , Hsu Shou-Yi , Lin Yu-An , Shih Chun-Ming , Huang Chun-Yao , Wang Kuo-Hsien , Lee Ai-Wei
Published: April 2022

Skin is an important organ that mainly functions as a barrier. Skin diseases can damage a person’s self-confidence and reduce their willingness to socialize, as well as their social behavior and willingness. When the skin appearance is abnormal, in addition to affecting the quality of life, it often leads to personal, social, and psychological dysfunction and even induces depression. Psoriasis and atopic dermatitis are common chronic skin diseases. Their prevalence in the world is 3–10%, and there is an increasing trend year by year. These congenital or acquired factors cause the dysfunction of the immune system and then destroy the barrier function of the skin. Because these patients are flooded with a variety of inflammatory mediators, this causes skin cells to be in chronic inflammation. Therefore, psoriasis and atopic dermatitis are also considered systemic chronic inflammatory diseases. In the healthcare systems of developed countries, it is unavoidable to spend high costs to relieve symptoms of psoriasis and atopic dermatitis patients, because psoriasis and atopic dermatitis have a great influence on individuals and society. Giving a lot of attention and developing effective treatment methods are the topics that the medical community must work on together. Therefore, we used a narrative review manuscript to discuss pathogenesis, clinical classification, incidence, and treatment options, including topical medication, systemic therapeutics, immunosuppressive medication for psoriasis, and atopic dermatitis, as well as also comparing the differences between these two diseases. We look forward to providing readers with comprehensive information on psoriasis and atopic dermatitis through this review article.
Trappc9 Deficiency Impairs the Plasticity of Stem Cells
Authors: Usman Muhammad , Li Yan , Ke Yuting , Chhetri Gaurav , Islam Md Ariful , Wang Zejian , Li Xueyi
Published: April 2022

Genetic mutations of trappc9 cause intellectual disability with the atrophy of brain structures and variable obesity by poorly understood mechanisms. Trappc9-deficient mice develop phenotypes resembling pathological changes in humans and appear overweight shortly after weaning, and thus are useful for studying the pathogenesis of obesity. Here, we investigated the effects of trappc9 deficiency on the proliferation and differentiation capacity of adipose-derived stem cells (ASCs). We isolated ASCs from mice before overweight was developed and found that trappc9-null ASCs exhibited signs of premature senescence and cell death. While the lineage commitment was retained, trappc9-null ASCs preferred adipogenic differentiation. We observed a profound accumulation of lipid droplets in adipogenic cells derived from trappc9-deficient ASCs and marked differences in the distribution patterns and levels of calcium deposited in osteoblasts obtained from trappc9-null ASCs. Biochemical studies revealed that trappc9 deficiency resulted in an upregulated expression of rab1, rab11, and rab18, and agitated autophagy in ASCs. Moreover, we found that the content of neural stem cells in both the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus vastly declined in trappc9-null mice. Collectively, our results suggest that obesity, as well as brain structure hypoplasia induced by the deficiency of trappc9, involves an impairment in the plasticity of stem cells.
The Genome-Wide Identification of Long Non-Coding RNAs Involved in Floral Thermogenesis in Nelumbo nucifera Gaertn
Authors: Jin Jing , Zou Yu , Wang Ying , Sun Yueyang , Peng Jing , Ding Yi
Published: April 2022

The sacred lotus (Nelumbo nucifera Gaertn.) can maintain a stable floral chamber temperature when blooming, despite ambient temperature fluctuations; however, the long non-coding RNAs (lncRNAs) involved in floral thermogenesis remain unclear. In the present study, we obtain comprehensive lncRNAs expression profiles from receptacles at five developmental stages by strand-specific RNA sequencing to reveal the lncRNAs regulatory mechanism of the floral thermogenesis of N. nucifera. A total of 22,693 transcripts were identified as lncRNAs, of which approximately 44.78% had stage-specific expression patterns. Subsequently, we identified 2579 differential expressed lncRNAs (DELs) regulating 2367 protein-coding genes mainly involved in receptacle development and reproductive process. Then, lncRNAs with floral thermogenesis identified by weighted gene co-expression network analysis (WGCNA) were mainly related to sulfur metabolism and mitochondrial electron transport chains. Meanwhile, 70 lncRNAs were predicted to act as endogenous target mimics (eTMs) for 29 miRNAs and participate in the regulation of 16 floral thermogenesis-related genes. Our dual luciferase reporter assays indicated that lncRNA LTCONS_00068702 acted as eTMs for miR164a_4 to regulate the expression of TrxL2 gene. These results deepen our understanding of the regulation mechanism of floral thermogenesis by lncRNAs and accumulate data for further research.
The Anti-Atherosclerosis Effect of Anakinra, a Recombinant Human Interleukin-1 Receptor Antagonist, in Apolipoprotein E Knockout Mice
Authors: Ku Eu Jeong , Kim Bo-Rahm , Lee Jee-In , Lee Yun Kyung , Oh Tae Jung , Jang Hak C. , Choi Sung Hee
Published: April 2022

Interleukin (IL)-1β plays an important role in atherosclerosis pathogenesis. We aimed to investigate the effect of anakinra, a recombinant human IL-1 receptor antagonist, on the progression of atherosclerosis in apolipoprotein E knockout (ApoE–/–) mice. ApoE–/– mice (8-week male) were treated with saline (control), anakinra 10, 25, and 50 mg/kg, respectively (n = 10 in each group). Mice were fed a standard chow (4 weeks) followed by an atherogenic diet (35kcal% fat, 1.25% cholesterol, 12 weeks). Atheromatous plaques in ApoE–/– mice and the expression of inflammatory genes and signaling pathways in human umbilical vein endothelial cells (HUVECs), rat aortic smooth muscle cells (RAOSMCs), and 3T3-L1 adipocytes were assessed. Anakinra reduced the plaque size of the aortic arch (30.6% and 25.2% at the 25 mg/kg and 50 mg/kg doses, both p < 0.05) and serum triglyceride in ApoE–/– mice and suppressed inflammatory genes (IL-1β and IL-6) expressions in HUVECs and RAOSMCs (all p < 0.05). In RAOSMCs, anakinra reduced metalloproteinase-9 expression in a dose-dependent manner and inhibited cell migration. Anakinra-treated mice exhibited trends of lower CD68+ macrophage infiltration in visceral fat and monocyte chemoattractant protein-1 expression was reduced in 3T3-L1 adipocytes. Anakinra could be a useful component for complementary treatment with a standard regimen to reduce the residual cardiovascular risk.
Macrophage Migration Inhibitory Factor (MIF) as a Stress Molecule in Renal Inflammation
Authors: Kong Yao-Zhong , Chen Qiyan , Lan Hui-Yao
Published: April 2022

Renal inflammation is an initial pathological process during progressive renal injury regardless of the initial cause. Macrophage migration inhibitory factor (MIF) is a truly proinflammatory stress mediator that is highly expressed in a variety of both inflammatory cells and intrinsic kidney cells. MIF is released from the diseased kidney immediately upon stimulation to trigger renal inflammation by activating macrophages and T cells, and promoting the production of proinflammatory cytokines, chemokines, and stress molecules via signaling pathways involving the CD74/CD44 and chemokine receptors CXCR2, CXCR4, and CXCR7 signaling. In addition, MIF can function as a stress molecule to counter-regulate the immunosuppressive effect of glucocorticoid in renal inflammation. Given the critical position of MIF in the upstream inflammatory cascade, this review focuses on the regulatory role and molecular mechanisms of MIF in kidney diseases. The therapeutic potential of targeting MIF signaling to treat kidney diseases is also discussed.
Risk Assessment and Area-Wide Crop Rotation to Keep Western Corn Rootworm below Damage Thresholds and Avoid Insecticide Use in European Maize Production
Authors: Furlan Lorenzo , Chiarini Francesca , Contiero Barbara , Benvegnù Isadora , Horgan Finbarr G. , Kos Tomislav , Lemić Darija , Bažok Renata
Published: April 2022

The Western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, has been a serious quarantine pest to maize in Europe since the mid-1990s. The integrated pest management of WCR requires an accurate knowledge of the factors that contribute most to risks of crop damage, as well as knowledge of effective area-wide strategies based on agronomic measures, such as crop rotation. In Italy and Croatia, agronomic and cultural factors in fields damaged by WCR were evaluated through a long-term survey. Based on the survey results, high-WCR densities contribute most to risks of damage to maize. Extensive field research in north-eastern Italy compared large areas of continuous maize production with areas under different crop rotation systems (i.e., a structural one with one-time maize planting in a three-year rotation and a flexible one with continuous maize planting interrupted when beetle populations exceed the threshold). The objective was to evaluate the effectiveness of different rotation regimes as possible best practices for WCR management. Captures of beetles in yellow sticky traps, root damage, larval densities, and damage to maize plants (e.g., lodging) were assessed at the center of each area. The results demonstrated the both structural and flexible crop rotation systems were effective strategies for maintaining WCR below damage threshold densities without the need for insecticides.
A Rare Case of Polysplenia Syndrome Associated with Severe Cardiac Malformations and Congenital Alveolar Dysplasia in a One-Month-Old Infant: A Complete Macroscopic and Histopathologic Study
Authors: Mohor Cosmin Ioan , Fleaca Sorin Radu , Oprinca Muja Alexandra , Oprinca George Calin , Roman Mihai Dan , Chicea Radu , Boicean Adrian Gheorghe , Dura Horatiu , Tanasescu Ciprian , Ion Nicolas Catalin Ionut , Faur Mihai , Bacila Ciprian Ionut , Batar Florina , Mohor Calin Ilie
Published: April 2022

Polysplenia syndrome represents a type of left atrial isomerism characterized by multiple small spleens, often associated with cardiac malformations and with situs ambiguus of the abdominal organs. The case presented is of a one-month-old male infant, weighing approximately 3000 g, born at the County Clinical Emergency Hospital of Sibiu, who was hospitalized from birth until death. The patient suffered cardio-respiratory arrest due to severe hypoxia and septicemia on the background of a series of complex cardiac malformations associated with congenital abdominal organ anomalies. Examination of the body revealed a common atrium with complete atrioventricular canal defect, left ventricular hypertrophy, right ventricle hypoplasia, truncus arteriosus, superior vena cava duplication, bilobation of the lungs, situs ambiguous of the abdominal organs with right-sided stomach, a midline liver, gall bladder agenesis, multiple right-sided spleens and complete inversion of the intestines and pancreas. Histopathology concluded that the patient suffered cardiac lesions consistent with infantile lactic acidosis, as well as pulmonary modifications suggesting congenital alveolar dysplasia and altered hepatic architecture compatible with fibrosis.
Three-Dimensional Adaptive Image Compression Concept for Medical Imaging: Application to Computed Tomography Angiography for Peripheral Arteries
Authors: Fahrni Guillaume , Rotzinger David C. , Nakajo Chiaki , Dehmeshki Jamshid , Qanadli Salah Dine
Published: April 2022

Advances in computed tomography (CT) have resulted in a substantial increase in the size of datasets. We built a new concept of medical image compression that provides the best compromise between compression rate and image quality. The method is based on multiple contexts and regions-of-interest (ROI) defined according to the degree of clinical interest. High priority areas (primary ROIs) are assigned a lossless compression. Other areas (secondary ROIs and background) are compressed with moderate or heavy losses. The method is applied to a whole dataset of CT angiography (CTA) of the lower extremity vasculature. It is compared to standard lossy compression techniques in terms of quantitative and qualitative image quality. It is also compared to standard lossless compression techniques in terms of image size reduction and compression ratio. The proposed compression method met quantitative criteria for high-quality encoding. It obtained the highest qualitative image quality rating score, with a statistically significant difference compared to other methods. The average compressed image size was up to 61% lower compared to standard compression techniques, with a 9:1 compression ratio compared with original non-compressed images. Our new adaptive 3D compression method for CT images can save data storage space while preserving clinically relevant information.
Insights into the Role of Neutrophils and Neutrophil Extracellular Traps in Causing Cardiovascular Complications in Patients with COVID-19: A Systematic Review
Authors: Nappi Francesco , Bellomo Francesca , Avtaar Singh Sanjeet Singh
Published: April 2022

Background: The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV-2 virus has resulted in significant mortality and burdening of healthcare resources. While initially noted as a pulmonary pathology, subsequent studies later identified cardiovascular involvement with high mortalities reported in specific cohorts of patients. While cardiovascular comorbidities were identified early on, the exact manifestation and etiopathology of the infection remained elusive. This systematic review aims to investigate the role of inflammatory pathways, highlighting several culprits including neutrophil extracellular traps (NETs) which have since been extensively investigated. Method: A search was conducted using three databases (MEDLINE; MEDLINE In-Process & Other Non-Indexed Citations and EMBASE). Data from randomized controlled trials (RCT), prospective series, meta-analyses, and unmatched observational studies were considered for the processing of the algorithm and treatment of inflammatory response during SARS-CoV-2 infection. Studies without the SARS-CoV-2 Infection period and case reports were excluded. Results: A total of 47 studies were included in this study. The role of the acute inflammatory response in the propagation of the systemic inflammatory sequelae of the disease plays a major part in determining outcomes. Some of the mechanisms of activation of these pathways have been highlighted in previous studies and are highlighted. Conclusion: NETs play a pivotal role in the pathogenesis of the inflammatory response. Despite moving into the endemic phase of the disease in most countries, COVID-19 remains an entity that has not been fully understood with long-term effects remaining uncertain and requiring ongoing monitoring and research.
The Effects of COVID-19 on Clinical Outcomes of Non-COVID-19 Patients Hospitalized for Upper Gastrointestinal Bleeding during the Pandemic
Authors: Pausawasdi Nonthalee , Manomaiwong Ekawat , Kaosombatwattana Uayporn , Karaketklang Khemajira , Charatcharoenwitthaya Phunchai
Published: April 2022

This study aims to investigate the effects of COVID-19 on clinical outcomes of non-COVID-19 patients hospitalized for upper gastrointestinal bleeding (UGIB) during the pandemic. A retrospective review is conducted. We recruited patients with UGIB admitted during the pandemic’s first wave (April 2020 to June 2020), and the year before the pandemic. The outcomes between the two groups were compared using propensity score matching (PSM). In total, 60 patients (pandemic group) and 460 patients (prepandemic group) are included. Patients admitted during the pandemic (mean age of 67 ± 14 years) had a mean Glasgow–Blatchford score of 10.8 ± 3.9. They were older (p = 0.045) with more underlying malignancies (p = 0.028), had less history of NSAID use (p = 0.010), had a lower platelet count (p = 0.007), and had lower serum albumin levels (p = 0.047) compared to those admitted before the pandemic. Esophagogastroduodenoscopy (EGD) was performed less frequently during the pandemic (43.3% vs. 95.4%, p < 0.001). Furthermore, the procedure was less likely to be performed within 24 h after admission (p < 0.001). After PSM, admissions during the pandemic were significantly associated with decreased chances of receiving an endoscopy (adjusted odds Ratio (OR), 0.02; 95% CI, 0.003–0.06, p < 0.001) and longer hospital stay (adjusted OR, 2.17; 95% CI, 1.13–3.20, p < 0.001). Additionally, there was a slight increase in 30-day mortality without statistical significance (adjusted OR, 1.92; 95% CI, 0.71–5.19, p = 0.199) and a marginally higher rebleeding rate (adjusted OR, 1.34; 95% CI, 0.44–4.03, p = 0.605). During the pandemic, the number of EGDs performed in non-COVID-19 patients with UGIB decreased with a subsequent prolonged hospitalization and potentially increased 30-day mortality and rebleeding rate.
Towards a Didactic Concept for Heterogeneous Target Groups in Digital Learning Environments—First Course Implementation
Authors: Katzensteiner Matthias , Vogel Stefan , Hüsers Jens , Richter Jendrik , Bott Oliver J.
Published: April 2022

In the context of the ongoing digitization of interdisciplinary subjects, the need for digital literacy is increasing in all areas of everyday life. Furthermore, communication between science and society is facing new challenges, not least since the COVID-19 pandemic. In order to deal with these challenges and to provide target-oriented online teaching, new educational concepts for the transfer of knowledge to society are necessary. In the transfer project “Zukunftslabor Gesundheit” (ZLG), a didactic concept for the creation of E-Learning classes was developed. A key factor for the didactic concept is addressing heterogeneous target groups to reach the broadest possible spectrum of participants. The concept has already been used for the creation of the first ZLG E-Learning courses. This article outlines the central elements of the developed didactic concept and addresses the creation of the ZLG courses. The courses created so far appeal to different target groups and convey diverse types of knowledge at different levels of difficulty.
Tumor Stimulus-Responsive Biodegradable Diblock Copolymer Conjugates as Efficient Anti-Cancer Nanomedicines
Authors: Šubr Vladimír , Pola Robert , Gao Shanghui , Islam Rayhanul , Hirata Takuma , Miyake Daiki , Koshino Kousuke , Zhou Jian-Rong , Yokomizo Kazumi , Fang Jun , Etrych Tomáš
Published: April 2022

Biodegradable nanomedicines are widely studied as candidates for the effective treatment of various cancerous diseases. Here, we present the design, synthesis and evaluation of biodegradable polymer-based nanomedicines tailored for tumor-associated stimuli-sensitive drug release and polymer system degradation. Diblock polymer systems were developed, which enabled the release of the carrier drug, pirarubicin, via a pH-sensitive spacer allowing for the restoration of the drug cytotoxicity solely in the tumor tissue. Moreover, the tailored design enables the matrix-metalloproteinases- or reduction-driven degradation of the polymer system into the polymer chains excretable from the body by glomerular filtration. Diblock nanomedicines take advantage of an enhanced EPR effect during the initial phase of nanomedicine pharmacokinetics and should be easily removed from the body after tumor microenvironment-associated biodegradation after fulfilling their role as a drug carrier. In parallel with the similar release profiles of diblock nanomedicine to linear polymer conjugates, these diblock polymer conjugates showed a comparable in vitro cytotoxicity, intracellular uptake, and intratumor penetration properties. More importantly, the diblock nanomedicines showed a remarkable in vivo anti-tumor efficacy, which was far more superior than conventional linear polymer conjugates. These findings suggested the advanced potential of diblock polymer conjugates for anticancer polymer therapeutics.
Human Papillomavirus Infection and the Risk of Erectile Dysfunction: A Nationwide Population-Based Matched Cohort Study
Authors: Juang Sin-Ei , Ma Kevin Sheng-Kai , Kao Pei-En , Wei James Cheng-Chung , Yip Hei-Tung , Chou Mei-Chia , Hung Yao-Min , Chin Ning-Chien
Published: April 2022

Background: Male patients with genital warts are known for higher rates of sexual dysfunction. This study was conducted to investigate whether human papillomaviruses (HPV) infection is associated with an increased risk of erectile dysfunction (ED). Methods: Patients aged over 18 with HPV infection (n = 13,296) and propensity score-matched controls (n = 53,184) were recruited from the Longitudinal Health Insurance Database (LHID). The primary endpoint was the diagnosis of ED. Chi-square tests were used to analyze the distribution of demographic characteristics. The Cox proportional hazards regression was used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) for the development of ED in both groups, after adjusting for sex, age, relevant comorbidities, co-medication, and surgery. Results: ED developed in 181 patients of the study group. The incidence density of ED was 2.53 per 1000 person-years for the HPV group and 1.51 per 1000 person-years for the non-HPV group, with an adjusted HR (95% CI) of 1.63 (1.37–1.94). In stratification analysis, adjusted HR of diabetes-, chronic obstructive pulmonary disease (COPD-), and stroke-subgroup were 2.39, 2.51, and 4.82, with significant p values for interaction, respectively. Sensitivity analysis yields consistent findings. Conclusions: The patients with HPV infection had a higher risk of subsequent ED in comparison to the non-HPV controls. The mechanism behind such association and its possible role in ED prevention deserves further study in the future.
Implementation of Green Infrastructure in Existing Urban Structures: Tracking Changes in Ferencváros, Budapest
Authors: Dantas Gabriel Silva , Nagy Ildikó Réka Báthoryné , Nogueira Pedro Brizack
Published: April 2022

Understanding the resilience of urban forms as a latent force that drives a place’s physical characterization and social cohesion is essential for defining successful adaptive processes of pre-existing urban fabrics. Budapest’s ninth district (Ferencváros) is an outstanding example of transforming a complex historical urban context, which underwent renovation strategies guided by maintaining and enhancing essential morphological elements. Courtyards have great relevance in conditioning the well-being in areas of high occupational density, especially in terms of accessibility to urban green infrastructure. In the case of Ferencváros, they were reframed to add new layers of use and to improve territorial integration by unifying smaller private courtyard unities into more extensive communal areas, creating a comprehensive urban green network, preserving urban heritage, and increasing green coverage. This study assesses how this recent re-urbanization phenomenon is related to political changes in a post-socialist city. The conjuncture found in Ferencváros is unique, yet it can be applied in other similar contexts. The methodology applied to this study is supervised classification for the quantitative analysis of remote-sensing image data with GIS software assistance—a procedure rarely applied in medium-scale urban analysis. However, it was verified to be precise and effective in tracking morphological changes. The preliminary results indicate a significant intensification in greenery in the urban pattern, especially in the core areas of the blocks: the courtyards. After the intervention, green areas became more predominant, cohesive, and articulated.
Approach for Village Carbon Emissions Index and Planning Strategies Generation Based on Two-Stage Optimization Models
Authors: Huang Zishuo , Liu Yingfang , Gao Jing , Peng Zhenwei
Published: April 2022

With the implementation of China’s rural revitalization strategy, the social economy of villages is expected to fully develop; however, their carbon emissions must be controlled within a reasonable range. Realization of this goal is part of the guidance and control of village planning. Clarifying the coupling relationship between village land uses and rural carbon emissions is fundamental for low-carbon village planning. In this study, by exploring the relationships between carbon emissions factors, land-use types, and human activities, the reference range of carbon emissions coefficients for various land-use types in rural areas is obtained. Then, based on the interval values of carbon emissions coefficients, a two-stage optimization model for village carbon emissions analysis is established, which is used to generate the minimal value of village carbon emissions and planning schemes to achieve different carbon emissions target values. First, the smallest carbon emissions value for a certain village is obtained based on a linear programming model. Then, to analyze the planning scheme possibilities under different carbon emissions targets, an objective planning model (including various parameters) is constructed. Through this two-stage optimization model, the optimal planning scheme is set and corresponding planning indicators under different scenarios are obtained through a sensitivity analysis. Combined with a case study in Dongzhuang Village, Shanghai, the results indicate that, with continuous improvement of the basic national carbon emissions database, the range of carbon emissions coefficients for typical local land uses can be determined, and the carbon emissions and land-use types of villages can be co-planned using the two-stage optimization model. With the proposed model, the range of carbon emissions for villages and scenario analysis results considering carbon emissions values associated with various land-use planning schemes can be obtained, contributing greatly to low-carbon village planning.
Serum C-X-C Chemokine Ligand 1 Levels in Patients with Systemic Sclerosis: Relationship of Clinical and Laboratory Observations to Anti-CD20 Monoclonal Antibody Administration
Authors: Kawanabe Ruriko , Yoshizaki Ayumi , Matsuda Kazuki M. , Kotani Hirohito , Hisamoto Teruyoshi , Norimatsu Yuta , Kuzumi Ai , Fukasawa Takemichi , Ebata Satoshi , Yoshizaki-Ogawa Asako , Sato Shinichi
Published: April 2022

Objectives: To determine whether C-X-C chemokine ligand 1 (CXCL1), which is a potent neutrophil chemoattractant and activator that plays important role in inflammation, is elevated in patients with systemic sclerosis (SSc) and whether it is associated with the clinical features and disease activity of patients with SSc. In addition, to determine whether the changes in serum CXCL1 levels before and after treatment correlate with changes in disease activity in SSc patients who received an anti-CD20 monoclonal antibody drug. Patients and method: We examined patient serum collected in the DesiReS trial, which was a double-blind, parallel-group, randomized, placebo-controlled, multicenter, phase II clinical trial. In the trial, patients were randomly allocated to the drug or placebo group and received 375 mg/m2 of an anti-CD20 antibody, rituximab, or placebo once a week for four weeks. We obtained serum samples from 47 patients administered at our hospital, including 3 males and 44 females, the median age of 48 years, range 27–71 years, with 42 diffuse cutaneous SSc and 5 with limited cutaneous SSc. Serum CXCL1 levels were measured using multiplex immunoassay in patient serum before and 24 weeks after administration and also in serum from 33 healthy controls. Results: Serum CXCL1 levels were significantly higher in SSc patients (mean 25.70 ng/mL; 95% confidence interval (CI) 18.35–33.05 ng/mL) than in the healthy controls (15.61 ng/mL; 95% CI 9.73–21.51 ng/mL). In addition, SSc patients with elevated CXCL1 levels had a significantly higher percentage of area occupied with interstitial shadows (p < 0.05), increased serum levels of surfactant protein (SP)-A (p < 0.05), SP-D (p < 0.05), Krebs von den Lungen-6 (p < 0.01), and C-reactive protein (p < 0.05) compared to those with normal levels. Furthermore, defining Δ as the value after rituximab administration minus the value before rituximab administration, baseline serum CXCL1 levels correlated with Δ percent predicted diffusing capacity for carbon monoxide (p < 0.01). In addition, ΔCXCL1 correlated with ΔSP-A (p < 0.05). Similarly, serum CXCL1 levels after rituximab administration correlated with percent predicted forced vital capacity (p < 0.05) and serum SP-D levels (p < 0.05) after rituximab. Conclusions: Our results suggest that serum CXCL1 is associated with the disease activity of SSc-ILD, and high serum CXCL1 levels are one of the predictors of improvement in SSc-ILD with rituximab.
Facile Preparation of a Novel Vanillin-Containing DOPO Derivate as a Flame Retardant for Epoxy Resins
Authors: Chen Liping , Luo Zhonglin , Wang Biaobing
Published: April 2022

A novel bio-based flame retardant designated AVD has been synthesized in a one-pot process via the reaction of 9,10-dihydro-9-oxa-10-phospha-phenanthrene-10-oxide (DOPO), vanillin (VN), and 2- aminobenzothiazole (ABT). The structure of AVD was confirmed using Fourier transform infrared spectroscopy (FTIR), and 1H and 31P nuclear magnetic resonance spectroscopy (NMR). The curing process, thermal stability, flame retardancy, and mechanical properties of the epoxy resin (EP) modified with AVD have been investigated comprehensively. The extent of curing, the glass transition temperature and the crosslinking density of the blend decreased gradually with increasing AVD content. The thermogravimetric analysis (TGA) was used to demonstrate that the presence of AVD reduced the thermal decomposition rate for EP and enhanced the formation of carbon residue during resin decomposition. A blend of 7.5 wt% AVD (0.52% phosphorus) displays a UL-94V-0 rating and a LOI of 31.1%. Reduction of the peak heat release rate, total heat release rate and total smoke production was 41.26%, 35.70%, and 24.03%, respectively, as compared to the values for pure EP. The improved flame retardancy of the flame retardant epoxy (FREP) may be attributed to the formation of a compact and continuous protective char layer into the condensed phase as well as the release of non-combustible gases and phosphorus-containing radicals from the decomposition of AVD in the gas phase. AVD is a new and efficient biobased flame retardant for epoxy with great prospects for industrial applications.
Static Electro-Mechanical Response of Axisymmetric One-Dimensional Piezoelectric Quasicrystal Circular Actuator
Authors: Zhang Linyan , Zhang Hongliang , Li Yang , Wang Jingbo , Lu Changguo
Published: April 2022

The piezoelectric effect of piezoelectric quasicrystalline materials is coexcited by phonon and phason fields. Piezoelectric quasicrystalline materials have excellent properties of both piezoelectric materials and quasicrystalline materials, which are expected to be used as actuators in the fields of aerospace, automotive, and intelligent manufacturing. Based on the three-dimensional elastic theory of piezoelectric quasicrystals, the state space equation for axisymmetric piezoelectric quasicrystal circular plate actuators is derived by using the state space method. Afterwards, the finite Hankel transformation is performed on the state equation, and a system of ordinary differential equations and corresponding boundary conditions are obtained. Finally, the exact solution of axisymmetric bending of one-dimensional hexagonal piezoelectric quasicrystal circular actuators under generalized elastic simply supported boundary conditions is obtained by using the propagator matrix method. Numerical results are given to compare the degradation results in this paper with those in the literature, and present the influences of the thickness-to-span ratio and stacking sequence on the phonon, phason, and electric fields when the surface of the laminated circular actuators is subjected to mechanical load. The exact solution obtained does not introduce any deformation assumption; therefore, the exact solution can provide references for numerical calculations of the mechanical behavior of piezoelectric quasicrystals.
Numerical and Experimental Studies of Mechanical Performance and Structural Enhancement of Industrial Building SSMRs
Authors: Ji Koochul , Choi Hyok Chu , Kwon Kyungrok , Kong Jung Sik
Published: April 2022

In response to the increasing demands of high-technology industrial buildings, renovated standing seam metal roofs (SSMRs) are widely used in the construction of such buildings due to their superior performance regarding heat insulation and waterproofing. However, studies to identify realistic mechanical performance and structural defects in newly applied SSMRs are still limited due to their recent development. In our previous full-scale experiment, the ultimate failure of the roof under wind pressure corresponded to mid-clip failure rather than end clip failure and seam separation; therefore, in this study, the lab-scale experimental programs mainly focused on the mid-clip and the metal roof sheet. Here, the plastic saddle type of the SSMR was chosen as the lab-scale experiment specimen under various loading speeds and angled plastic saddle conditions. The JC material properties were calibrated against experimental results and simulated to predict the dynamic failure response of SSMRs. An additional experimental study was conducted to identify the effect of strengthening SSMRs with wind clips, which showed that 20.77% of the peak load was enhanced after reinforcing the SSMR with wind clips. On the basis of this result, the failure wind speed was computed according to ASCE 7–10 standards with the assumption of a wind clip installed on the corner and edge of the roof panel, indicating that the failure wind speed increased with the wind clip by about 6 to 7 m/s. The current research results suggest a methodology for enhancing the structural performance of renovated industrial building SSMRs.