bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity, Distribution and Abundance of Scleractinian Coral in District-based Marine Protected Area Olele, Bone Bolango, Gorontalo - Indonesia
Authors: Reza Akbar , Peni Sancayaningsih Retno
Published: May 2017

Despite its status as District-Based Marine Protected Area, studies pertaining coral reef biodiversity never been conducted in Olele that located in the Gulf of Tomini, biodiversity hotspot in the Coral Triangle. Hence, we aims to investigate diversity, distribution and abundance of scleractinian coral, underpinning the health of reef ecosystems. This study was conducted using Line Intercept Transect on three research stations which were divided into three main zonations, reef flat (3 m depth), upper reef slope (10 m depth) and reef slope (18 m to 20 m depth). Fifty meters long transect were laid in each zonation, thus total transect were nine. The results showed that there were 35 species of scleractinian coral from 12 families. Each zonation has a different pattern of abundance and dominant life form. Based on coral health assessment using Coral Mortality Index (CMI) and the ratio between living and dead coral coverage, coral reef ecosystems in Olele were in healthy condition with the range value 0.29 ± 0.1 and 3.60 ± 1.14. This preliminary study should be used as a basic reference for database and novel direction of conservation management strategy in District-Based Marine Protected Area Olele.

Keywords: district-based marine protected area; olele; lit; sclecartinia;life form; zonation.
Isolation Hope and Threat of Mangrove Restoration Program in Bogowonto Lagoon, Yogyakarta - Indonesia (2002-2014)
Authors: Firaswan Abdullah , Aryandari Rindra , Reza Akbar , Faizah Nur , Rachman Nur , Fitri Ramadhani Aqmarina , Sulthon Andalas Muhammad
Published: May 2017

Bogowonto Lagoon  has  the  largest  mangrove ecosystem in  southern coast  of  Yogyakarta, Indonesia. Several activities on mangrove restoration have been conducted to escalate this remnant mangrove forest area. Conversely, rapid increase of shrimp pond area become threat for mangrove ecosystem in Bogowonto lagoon since the early of 21st century. Hence, This study is aimed to investigate mangrove composition, distribution and abundance, progress of mangrove restoration program and shrimp pond extensification in 2002 to 2014. Conducted from April 2015 to May 2015, this research consists of several steps namely survey, vegetation analysis, literature study, sociological study using questionnaire, measurement of mangrove and shrimp pond area using Landsat 8 imageries and Arc GIS 10.1. We determined 15 sites along Bogowonto Lagoon using random sampling by quadratic plot (10 m × 10 m for tree, 5 m ×5  m for sapling) and total counts (for seedling). The result showed that there were seven species of mangrove and six species of associate mangrove. Rhizopora mucronata Lamb. was dominant in each growthform with clumped distribution. R. mucronata and Avicennia marina (Forssk.) Vierh. also dominate from backswamp to rivermouth. Satellite imageries revealed that there was a hope due to upward trends of mangrove area in last 12 yr (approximately 5 ha). However, the significant increase sof shrimp pond area (approximately 34 ha) surrounding mangrove area become current and future threat. Based on sociological study, most of people around Bogowonto Lagoon have high awareness, but less participated on mangrove restoration program. Besides, the river mouth covered by sand become another obstacle of it.

 

Keywords: Bogowonto lagoon, mangrove restoration, shrimp pond, vegetation analysis
Pun1 Gene Isolation from Capsicum frutescens L. cv Cakra Hijau
Authors: Khasna Elhah Nailul , Zairina Shelly , Juliandari Ria Reinnata , Sulasmi Eko Sri , Listyorini Dwi
Published: May 2017

Capsicum frutescens L. is one of chili peppers with high pungency. Capsicum frutescens has several cultivars, one of those is C. frutescens cv. Cakra Hijau. This cultivars is known resistance to pests and diseases as well. Pungency is due to the accumulation of capsaicinoids. Pun1 is an important gene responsible for pungency. The full-leght genomic sequence of Pun1 is 1897 bp, containing two exons of 738 bp and 590 bpand one intron of 348 bp in between. This study was aimed to isolate Pun1 gene that free from intron. mRNA was isolated with TriReagentâ furthermore RT-PCR method used Qiagent One-Step RT-PCR and two pairs of primer : F1/R1 (F15’-ATG-GCT-TTT-GCA-TTA-CCA-TCA-3’ / R15’- CTT-AGC-TCG-AAG-TGC-ATC-TA-3’) and F2/R2 (F25’-GAA-GGT-GGC-AGA-AGA-ATC-AG-3’/R25’-TTA-GGC-AAT-GAA-CTC-AAG-GA-3’). The result of this study are isolated 738 bp exon-1 and 590 bp exon 2 of Pun1 gene.

 

Keywords: Capsaicin; Capsicum frutescens L. cv. Cakra Hijau; exon; Pun1 gene 
Sembur Karo: Karo’s Traditional Medicine as Burns Injury Alternative Treatment with Rattus norvegicus as Model
Authors: . Hennisa , Risa Novia , . Juliany , Kelvin Shawn , Kalati Tyas Delilla , Lukitaningsih Raden Rara Endang , Burlakovs Juris
Published: May 2017

Burns injury can be caused by flame, heat, chemical and electricity and it remains as one of the most serious problems in the medical world. Burns injury can be treated with traditional medicine. One of them is Sembur Karo. The objective of this research was to investigate the Sembur Karo potency for burns injury treatment. Sembur Karo obtained from North Sumatera was identified its plant composition and analyzed for bioactive compounds; flavonoid, steroid, and tannin. Twenty five rats (Rattus norvegicus) were used. The rats were divided into five groups of five. The first group used as a control without treatment, the second group are sprayed with Sembur Karo, while the third and the fourth groups were applied with Sembur Karo added with water and human saliva respectively. The last group were treated with Bioplacentone. All treatments were done once a day for               3 wk. Each week, one rat from all group were sacrificed with skins were taken for histology specimen. From this research, it was concluded that bioactive compounds in Sembur Karo were isoquersetin, sterol, and condensed tannin. The skin histology showed that the group which sprayed with Sembur Karo had the most effective effect among all groups.

 

Keywords: burn injury; burn injury recovery; Sembur Karo.
The Effect of Light Treatment and Media Combination on Luminescence Endurance of Bioluminescent Bacteria Isolated from Squid [Loligo duvacelli ( D'Orbigny, 1835)]
Authors: Werdani Ika Diana , Choirunnisa Hesti Nur , Sholikah Nur Hidayatus , Prabaningtyas Sitoresmi
Published: May 2017

Some bacteria emits light in the dark. The aim of this research is to find lighting duration and medium composition which produce the most enduring luminescence. The results showed that one of the bacteria isolated from light organ of Loligo duvacelli (D'Orbigny, 1835) squid are able to emit light. The isolation use trisalt solution and cultured into nutrient agar with addition of NaCl emits the longest blue-green light duration under a 1:1 dark-light shift incubation 8.3 d. Meanwhile under a total light, total dark, and in the oven incubator it illuminate for 4.2 d, 3.6 d and 2.6 d, respectively. The combination of beef extract + peptone + a commercial agar + NaCl generate the longest duration of luminescence when incubate in the oven incubator (3,2 d). Meanwhile it illuminated for 2.6 d and 1.7 d on NA + NaCl and NC + NaCl media, respectively, but it failed to illuminate on PCA, NA, and green beans extract + commercial agar + NaCl media. Based on this research we conclude that a commercial agar is potential to replace a technical bacterial agar function.

 

Keywords: bioluminescence; commercial agar; light treatments;  Loligo duvaceli  (D'Orbigny, 1835); medium treatment.
Potency of Endo-Exopolysaccharide from Porphyridium cruentum (S.F.Gray) Nägeli as Antioxidant (DPPH) and Biological Toxicity (BSLT)
Authors: Agustini Ni Wayan Sri , . Kusmiati
Published: May 2017

Sources of antioxidants are abundant in nature, one of which is derived from microalgae. Microalgae species that have potentially to be developed as a producer of antioxidant compounds is Porphyridium cruentum (S.F.Gray) Nägeli. P. cruentum is a red microalga (Rhodophyceae) and has been known as polysaccharides producer (exopolysaccharide and endopolysaccharide) and as an antioxidant. This study aims to determine antioxidant activity of the extracts and biological toxicity of endo- and exopolysaccharide P. cruentum. The antioxidant activity use reduction method of free radicals (DPPH), whereas toxicity uses Brine Shrimp Lethality Test. (BSLT). The result shows that there is an antioxidant activity with IC50 value of 50.586 5 mg · kg–1 (exopolysaccharide) and 145.998 8 mg · kg–1 (endopolysaccharide). In addition, the result indicates the toxic nature of the Artemia salina Leach. with LC50 values of 513.175 1 mg · kg–1 (exopolysaccharide) and 521.823 3 mg · kg–1  (endopolysaccharide). Therefore, endo- and exopolysaccharide produced by P. cruentum suitable to be used as an alternative source of natural antioxidants and potential for further developed as antitumor drugs.

Keywords: antioxidant, biological toxixity, endo-exopolysaccharide, Porphyridium cruentum (S.F.Gray) Nägeli.
The Growth and Antioxidative Responses of Sonchus oleraceus (Linnaeus, 1753) Under Cu(II), Pb(II), Cd(II) and Cr(VI) Stress Condition
Authors: Hidayaturrohmah Nur , Kasmiyati Sri , . Sucahyo , Vincēviča-Gaile Zane
Published: May 2017

Plants growing in soil containing heavy metal  polutan such as chromium (Cr), lead (Pb), cadmium (Cd) and copper (Cu)  will be stunted, and  increase production of Reactive Oxygen Species (ROS). In dealing with the excess amount of ROS, plants have an enzymatic defense system, using superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX). The aim of this study was to determine and analyze the Sonchus oleraceus (Linnaeus, 1753) plant response to heavy metals stress, seen from the growth and antioxidative defense enzymatically. Research carried out using a Completely Randomized Design (CRD) with four treatments and five replicates. The metal treatment was 10 mg · L–1. The presence of heavy metals in the growing medium significantly decreased the plant height and leaf area, so the impact is on the weight of wet and dry weight. The metal treatments of Cr, Cd, Pb and Cu increase the activity of SOD and APX enzymes but decrease the activity of the CAT enzyme. Chromium is a metal that has a significant influence on the growth and activity of SOD, APX, and CAT enzymes in S. oleraceus.

Keywords: antioxidative responses; growth; heavy metal; Sonchus oleraceus; toxicity.
Production and Characterization of Extracellular Protease from Bacillus sp. 140-B Isolated from Pineapple Plantation in Lampung, Indonesia
Authors: Laili Nur , Antonius Sarjiya
Published: May 2017

Bacillus sp. being industrially important organisms produces a wide variety of extracellular enzymes including protease. Bacillus sp. 140-B isolated from rhizosphere area of pineapple plantation in Lampung Province, Indonesia was tested for production of protease on skim milk agar medium. The aims of this study were to characterize and investigate some properties of  protease activity from Bacillus sp. 140-B. Bacillus sp. 140-B showed  protease activity qualitatively by clear zone diameter of 15.0 mm and the highest protease activity was 35.02 Unit · mg–1 protein at 14 h after incubation. Some properties of protease activities from Bacillus sp. 140-B including effects of temperature, pH and several metals were observed in this experiment. The protease activity from Bacillus sp. 140-B had optimum pH of 7,0 and the optimum temperature was 60 °C. Several metals which were evaluated on protease activity showed that Mn could increase protease activity, while other metals (Ca, Co, and Hg) showed decreasing protease activity of Bacillus sp. 140-B.

 

Keywords: Bacillus sp. 140-B; enzyme activity; enzyme properties; protease
Behavior and Ecological Study of Marine Birds in Alas Purwo National Park Southern Cost - Indonesia
Authors: Sholihah Raudatus
Published: May 2017

Alas Purwo National Park is habitat for marine birds and stopover for some the migratory birds. The purpose of this research was to determine behavior that could be observed in populations of marine birds and their habitat in the Alas Purwo National Park. The research location was in the Alas Purwo National Park southern coast, in region Plengkung to Cungur, Banyuwangi East Java. This research was done for 2 mo, July 21 to 30 and August 2015. The observations were observed in the morning (at 6:00 am to 9:00 am) and afternoon (15:00 pm to 18:00 pm). This study used a purposive sampling method to determine the location of the observations near the coast. The observations of marine birds habitat and behavior used survey methods and direct observation at the plot along the coastline. Identification and calculation of marine bird populations conducted in each observation plots. The plots exploration by foot and repeated three times. The behavior of marine birds that can be observed were birds clustered beach sunbathing on the beach, wallowing in the water, drinking and foraging in the surrounding water and flew low over the waters up to the mangrove areas for perch. Marine birds habitat are in mangrove and near the coast with moderate temperatures, high air humidity, low water temperature, and light intensity is sufficient.

Keywords: Alas Purwo national park; behavior; ecological study; habitat; marine bird.
SMART-RDA: A Galaxy Workflow for RNA-Seq Data Analysis
Authors: Aditama Redi , Tanjung Zulfikar Achmad , Sudania Widyartini Made , Liwang Toni
Published: May 2017

RNA-seq using the Next Generation Sequencing (NGS) approach is a common technology to analyze large-scale RNA transcript data for gene expression studies. However, an appropriate bioinformatics tool is needed to analyze a large amount of transcriptomes data from RNA-seq experiment. The aim of this study was to construct a system that can be easily applied to analyze RNA-seq data. RNA-seq analysis tool as SMART-RDA was constructed in this study. It is a computational workflow based on Galaxy framework to be used for analyzing RNA-seq raw data into gene expression information. This workflow was adapted from a well-known Tuxedo Protocol for RNA-seq analysis with some modifications. Expression value from each transcriptome was quantitatively stated as Fragments Per Kilobase of exon per Million fragments (FPKM). RNA-seq data of sterile and fertile oil palm (Pisifera) pollens derived from Sequence Read Archive (SRA) NCBI were used to test this workflow in local facility Galaxy server. The results showed that differentially gene expression in pollens might be responsible for sterile and fertile characteristics in palm oil Pisifera.

Keywords: FPKM; Galaxy workflow; Gene expression; RNA sequencing.
Gene Encoding Nitrilase from Soil Sample of Lombok Gold Mine Industry using Metagenomics Approach
Authors: Riffiani Rini , Sulistinah Nunik , Sunarko Bambang
Published: May 2017

This paper describes a efficient screening gene nitrilase from contaminated soil from Lombok gold mine industry. DNA was extracted directly from soil using the soil DNA isolation kit based on enzymatic, chemical and mechanical lysis. The existence of nitrilase gene in soil sample can be identified by nitrilase gene amplification using H1F˗H1R primer. BLASTN analysis result revealed that the nitrilase gene fragment which was amplified by H1F˗H1R primer has a high homology with Rhodococcus rhodochrous strain tg1˗A6 nitrilase gene. These amplification and DNA fragment sequencing results indicated that nitrilase gene existence on soil sample can be identified by metagenomic approach

 

Keywords: Metagenomics; nitrilase gene; Rhodococcus rhodochrous; soil.
Isolation of Dihydroflavonol-4-reductase (DFR) Gene in Dendrobium helix cv. Pomeo Brown
Authors: Anggraini Riska , Febriani Ayu Linda , Mazieda Mutia Naila , Al-Yamini Thobib Hasan , . Dahlia , Listyorini Dwi
Published: May 2017

Dendrobium helix cv. Pomeo Brown is one of the most attractive species in Spatulata section. This orchid is an epiphyte which has clustered stem, elliptic leaves, and erected flowers with brown petals. The brown color of D. helix cv. Pomeo Brown is due to the anthocyanin. Biosynthetic pathway of anthocyanin is regulated by several enzymes, including key enzyme dihydroflavonol-4-reductase (DFR) which is encoded by DFR gene. This study aimed to analyze the DFR gene from D. helix cv. Pomeo Brown by PCR using primer which were designed based on Dendrobium moniliforme. The sequences amplified using these primers could not be analyzed. Based on the sequence chromatograph, it can be suggested that the amplified DNA consists of several DFR fragments.

Keywords: Dendrobium helix cv. Pomeo Brown; anthocyanin; DFR gene.
Synthesis of cDNA Pun1 gene from Capsicum frutescens L. cv. Cakra Hijau
Authors: Zairina Shelly , Khasna Elhah Nailul , Juliandari Ria Reinnata , Sulasmi Eko Sri , Listyorini Dwi
Published: May 2017

Capsicum  frutescens L. cv. Cakra Hijau is a local cultivar that has been widely cultivated in Indonesia due to the pungency. Pungent on Capsicum is generated by capsaicin compound encoded by Pun1 gene. The sequences of Pun1 gene containing with two exons that located on the upstream and downstream, which are separated by introns in the middle. This study aimed to synthesis of cDNA of Pun1 gene from isolated total mRNA using two primers: F1/R1 (F1 5’- ATG GCT TTT GCA TTA CCA TCA -3’; and R1 5’-CTT AGC TCG AAG TGC ATC TA-3’) to synthesis the exon-1 sequences and F2/R2 (F2 5’-GAA GGT GGC AGA AGA ATC AG-3’; and R2 5’-TTA GGC AAT GAA CTC AAG GA-3’) to synthesis the exon-2 sequences. The cDNAs resulted from RT-PCR were visualized on 1.5 % agarose gel electrophoresis. From this study we obtained a 1323 bp fragment cDNA.

 

Keywords: Capsicum frutescens L. cv.Cakra Hijau; Pun1 gene; synthesis of cDNA.
The Processing Model in Making Tempeh Benguk [Mucuna pruriens (L.) DC] Containing High L-Dopa
Authors: Winarni Sri , Dharmawan Yudhy
Published: May 2017

“Koro benguk” [Mucuna pruriens (L.) DC] seed contains L-dopa which is able to repair spermatozoa quality.  The aim of this experiment is to analyze L-Dopa using HPLC in engineering manufacture of tempeh benguk. There are four engineering model’s variants of making tempeh benguk. The engineering process begins with selection of koro benguk seed, tools required, immersion, boiling, humidity, temperature and pH. The result shows that the process in making tempeh benguk which contains the highest concentration of L-Dopa (11 346.85 mg · kg–1) is white-colored  and intact koro benguk seed; one time immersion with replacing the water for 4  to 5 times/day (two days and two nights); one time boiling (< 1 h  until koro benguk seed can be opened); mixed with 10 mL lime juice/1.5 kg, with concentration of 0.83 % until pH 5, added with mashed teak leaves, and ended with the process of making tempeh benguk at room temperature (30 ºC), relative humidity room (65 %). Tempeh benguk which is produced is cleaner, whiter, nonperishable, and more tender.

Keywords: HPLC; L-dopa; modified process of making; seeds benguk [Mucuna pruriens (L.) DC]; tempeh.
Molecular Identification of Indigenous Lactic Acid Bacteria, Partial Purification and Characterization of β-Galactosidase Produced
Authors: Khusniati Tatik , . Sulistiania , Pamela Fury Bellen Nastitie , Falah Syamsul , Choliq Abdul
Published: May 2017

β-Galactosidase is enzyme which hydrolyze lactose to glucose and galactose, as lactose hydrolyzer. To know indigenous lactic acid bacteria (LAB) characteristics, indigenous LAB identification, partial purification and characterization of β-galactosidase produced was researched. LAB was molecularly identified, β-galactosidase partial purification was conducted by precipitation followed dialysis. β-Galactosidase  characterization was based on optimum activities of pH and temperature. The results show that LAB was identified as Lactobacillus plantarum B123. Optimum activity of precipited β-galactosidase was reached at 50 % ammonium sulphate. Activity and specific activity of 50 % precipited β-galactosidase were 95.675 U · mg–1 and 32.268 U · mg–1, respectively. Precipited β-galactosidase resulted a purification level of 3.99 fold, a yield of 38.73 %, and a specific activity of 32.27 U · mg–1 protein, while dialyzed β-galactosidase resulted 7.61 fold, 10.67 %, and 61.53 U· mg–1 protein. Optimum temperature and pH for crude β-galactosidase were found at 55 °C and 7.0, respectively, while that dialyzed β-galactosidase were optimized at 50 °C and 7.0. Based on partial purification and characterization, Lactobacillus plantarum B123 is indigenous LAB which good for production of β-galactosidase.

 

Keywords: characterization; dialysis; lactic acid bacteria; Lactobacillus plantarum B 123; β-galactosidase.
Secondary Metabolites Production by Actinomycetes and their Antifungal Activity
Authors: Dewi Tirta Kumala , Agustiani Dwi , Antonius Sarjiya
Published: May 2017

Wilt desease of banana caused by Fusarium oxysporum f.sp. cubense (FOC) is one of the most destructive deseases of banana in the tropics. Actinomycetes are the most economically and biotechnologically valuable prokaryotes able to produce wide range of bioactive secondary metabolites. The aims of the present study are to isolate and screen the actinomycetes with high potential ability to produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, Fusarium oxysporum f. sp. cubense. Two isolates from Lampung and Cianjur showed activity against fungi. The isolates designed as L.3.1 and CiIA5b. The metabolites from potent stain was produced by extraction of culture filtrate with ethyl acetate : methanol (4:1), it was tested for their antifungal activity by well diffusion method. Evidence for in vitro antibiosis of L.3.1 and CiIA5b isolates was demonstrated by the zone of fungal-growth inhibition. Production of secondary metabolites was analysis by thin layer chromatography (TLC) and bioautography assays. In this study, the metabolites from L.3.1 and CiIA5b have showed good antifungal activity.

 

Keywords: Actinomycetes; antifungal activity; bioautography; secondary metabolites; thin layer chromatography.
The Effect of Papaya (Carica papaya L.) Leaf Extract to the Number of Trypanosoma evansi Steel in Liver and Kidney of Mice (Mus musculus L. 1758)
Authors: Yahya Yudi , Nurliani Anni , Santoso Heri Budi
Published: May 2017

The purpose of this study proved the activity of the ethanolic extract of papaya leaf as anti-trypanosomiasis agent, determined the dose of extract which having an effect in inhibiting growth of T. evansi, and evaluated the histopathology of liver and kidney infected by Trypanosoma evansi Steel. Male Balb-c mice were infected by 3 × 107 T. evansi isolates. The native method was used to determine the level of parasitemia.The preparation was made using cytological smear method and paraffin method. The results showed that ethanolic extract of papaya leaf decreased the number of T. evansi. Ethanolic extract of papaya leaf at a dose of 300 mg · kg–1 BW is the most influential dose to reduce the number of T. evansi in liver and kidney of mice. Liver reveals degenerative change varying such as congestion, lipid degeneration, vacuolar degeneration, necrosis, and cellular infiltration. Kidney reveals tubular degeneration, necrosis, cellular infiltration, and glomerulonephritis.

 

Keywords: Carica papaya L.; kidney; liver; mice; Trypanosoma evansi Steel
Mus musculus (Linnaeus, 1758) Immune Responses Caused by Escherichia coli (Migula, 1895) Infection
Authors: Mitasari Zuni , Gofur Abdul , Listyorini Dwi
Published: May 2017

Escherichia coli (Migula, 1895) is a negative gram bacteria which have an ability to cause some deseases, such as meningitis, urinary tract infection and digestive tract infection. Lipopolysaccharide (LPS) in its cell wall induces immune responses in many ways. The objective of this study was to investigate the effect of E. coli infection on lymphocyte numbers and spleen weight in mice [Mus musculus (Linnaeus, 1758)]. Twenty five male mice were grouped into negative control, positive control group treated with PBS, and three experimental groups injected intraperitonially with E. coli 1.5 × 103 cfu/mL,1.5 × 105 cfu/mL or 1.5 × 107 cfu/mL respectively.  About 5 d after the injection, the spleen were collected. Spleen were weighed using digital balance, number of lymphocyte were counted using hemocytometer. E. coli infection significantly increase both weight of the spleen and number lymphocyte. In conclusion,  mice responded to E. coli infection by increasing the number of its lymphocyte.

 

Keywords: Escerechia coli (Migula, 1895); immune respone; infection; Mus musculus (Linnaeus, 1758).
STUDY OF THE EFFECT OF MEDICINAL PLANTS EXTRACTS ON THE INFECTED BLOOD
Authors: Kate Supriya , Chatrath Harsha M
Published: May 2017

Medicinal plants are used for treating and preventing the specific disease that affects the human being. There are several species of medicinal plants available in nature; amongst those researchers selected Basil, Zingiber, Azadirachtaindica, Curcuma longa, Carica Papaya, Allium sativum for research purpose. Natural compounds have been isolated from various parts such as leaves, fruits, stem, roots, and seeds of these plants. The present paper is the comparative study of variation in the physical properties like pH, critical solution temperature, and boiling point, etc. of these medicinal plants, individually, in combination ratios and also with the infected blood doped samples.
DIREITOS HUMANOS SOB AMEAÇA: ORGANIZAÇÕES PATOGÊNICAS, TRABALHO E SUBJETIVIDADE
Authors: Silva Eduardo Pinto e , Heloani Roberto , Piolli Evaldo
Published: May 2017

Objetiva-se, neste artigo, discutir os Direitos Humanos no processo civilizatório atual, e em que medida ele estaria fragilizado, no campo do trabalho, e caracterizado por quantofrenia e desqualificação dos que resistem ou não alcançam metas desumanas de produtividade. Consideramos a universalização dos Direitos Humanos um sinal evidente do processo civilizatório e condição para a democracia e concretização de uma ética balizada na tolerância e prática dialógica. No entanto, ao analisarmos as relações entre direitos humanos, ética e trabalho, nas organizações públicas e educacionais inclusas, e suas influências sobre a subjetividade, apontamos que a barbárie se inscreve no processo civilizatório. De forma contraditória aos princípios éticos e democráticos, são produzidas situações de assédio moral e formas de solidariedade mórbidas no trabalho, calcadas na humilhação e estigmatização.

Palavras-chave: Direitos Humanos. Assédio moral. Ttrabalho. Ssubjetividade. Gerencialismo.
SISTEMA DE ENSINO APOSTILADO: UM “CAVALO DE TROIA”?
Authors: Giacomini Raquel de Melo , Otto Clarícia
Published: May 2017

Neste artigo, o objetivo é compreender a política utilizada para a implantação do Sistema Educacional Família e Escola (SEFE), em escolas da rede municipal de ensino de Florianópolis (SC), entre 2009 e 2012. O método utilizado foi observação e participação nos cursos de formação continuada de professores, ministrados pelo SEFE, aplicação de 82 questionários e realização de oito entrevistas com professores de escolas que utilizam o SEFE. Como aportes teóricos, tomamos o conceito de estratégias, de acordo com Michel de Certeau; a produção de subjetividades, na acepção de Michel Foucault; e, a governamentalidade neoliberal, conforme indicado por Karla Saraiva e Alfredo Veiga-Neto. Dentre os resultados, inferimos que o SEFE adota estratégias de governamentalidade com características neoliberais, tais como: estímulo à competição, busca pela eficiência e qualidade, cumprimento de metas e práticas de meritocracia. Além disso, é possível identificar que a utilização do material didático do SEFE propicia a emergência de um profissional que atua de forma mecânica, o que pode conduzir a um paulatino processo de perda da autonomia docente.

Palavras-chave: Sistema Educacional Família e Escola. Estratégias. Governamentalidade neoliberal.
O USO DA (AUTO)BIOGRAFIA EM PESQUISAS BRASILEIRAS (2001 – 2010): A CONSOLIDAÇÃO DE UMA TENDÊNCIA METODOLÓGICA
Authors: Da Silva Marilda , Sgobbi Isabela Vicenzo , Carlindo Eva Poliana
Published: May 2017

O objetivo desta pesquisa é apresentar um mapeamento/balanço quantitativo que possibilita dar visibilidade ao uso que foi feito do recurso teórico-metodológico (auto)biografia por pesquisadores brasileiros no período de 2001 - 2010. Busca-se, assim, mostrar o movimento que consolidou esse recurso como tendência metodológica no referido período no âmbito da história epistemológica produzida em tal tempo no Brasil, nas Ciências Humanas e no Campo Educacional. Trata-se de uma pesquisa documental. As fontes foram 742 pesquisas: 514 mestrados e 228 doutorados. Usou-se o Banco de dados da CAPES. Os dados apontam que o uso do referido recurso metodológico consolidou uma tendência metodológica no âmbito da história epistemológica produzida no Brasil em tal tempo, abarcando as Ciências Humanas de modo geral e o Campo Educacional em particular. Nesse sentido, na década em questão, a média anual de produção foi de 74 pesquisas. Outrossim, os dados evidenciaram os nichos institucionais nucleares da consolidação da tendência.

Palavras-chave: (Auto)biografia. Produção. Ciências Humanas. Campo Educacional.
ASSOCIAÇÃO ENTRE AFETOS E REPRESENTAÇÕES ENVOLVIDAS NO AMBIENTE DE APRENDIZAGEM ESCOLAR
Authors: Osti Andreia , Noronha Ana Paula Porto
Published: May 2017

O presente estudo parte da premissa de que as dimensões afetivas envolvem o processo de planejamento e desenvolvimento das práticas pedagógicas e constituem fator importante na determinação da natureza das relações estabelecidas entre os estudantes e os diversos objetos de conhecimento. Nesse sentido, objetivou-se analisar como os alunos representam os aspectos afetivos do processo de ensino e aprendizagem e quais suas percepções sobre o ambiente da aprendizagem. Participaram da pesquisa 120 estudantes do 5° ano do ensino fundamental de escolas públicas municipais da Região Metropolitana de Campinas, sendo 60 alunos com satisfatório desempenho acadêmico e 60 com dificuldades de aprendizagem. Metodologicamente foram utilizados três instrumentos: Prova Psicopedagógica Par Educativo, Escala de Afetos Zanon e Escala de Expectativas do Professor. Os resultados revelaram, que alunos com dificuldade de aprendizagem se diferenciam significativamente daqueles com adequado desempenho. Alunos com dificuldades estabelecem menor vínculo com a aprendizagem escolar formal e com seus professores e por isso retratam situações não escolares, enquanto que alunos com desempenho satisfatório têm uma melhor percepção das expectativas de seus professores e demonstram ter uma relação mais afetiva com o ambiente escolar. Acredita-se que essa pesquisa contribui para o entendimento sobre a relação entre os sentimentos vivenciados por alunos no contexto da sala de aula e suas implicações para o desempenho acadêmico dos mesmos.

Palavras-chave: Psicologia positiva. Relações interpessoais. Experiências de aprendizagem.
Precarious Care Labor: Contradictory Work Regulations and Practices for Au Pairs in Sweden
Authors: Anving Terese , Eldén Sara
Published: May 2017

This paper focuses on the rules and regulations relating to au pair work in Sweden, and how these rules correlate with au pairing practices. The precarious position of au pairs has been highlighted before, but in addition, au pairs coming to Sweden also find themselves in an unclear work situation due to contradictory rules and regulations. While au pairs from outside the EU must apply for a work permit that defines their work as cultural exchange, this regulation does not apply to EU au pairs. As a consequence, we currently see the emergence of an almost completely unregulated— and growing—market for au pairing in Sweden. Drawing on a qualitative study of the private child care market in Sweden, this paper analyzes rules and regulations for au pairing, as well as how au pair working conditions are understood, negotiated, and realized by employing parents, and au pairs themselves. This is analyzed in relation to theoretical elaborations of paid and unpaid work, as well as discussions of care as a practice where ‘work’ and ‘emotion’ is inherently intertwined.
Thermal Signature Based Sleep Analysis Sensor
Authors: Seba Ali , Istrate Dan , Guettari Toufik , Ugon Adrien , Pinna Andrea , Garda Patrick
Published: May 2017

This paper address the development of a new technic in the sleep analysis domain. Sleep is defined as a periodic physiological state during which vigilance is suspended and reactivity to external stimulations diminished. We sleep on average between six and nine hours per night and our sleep is composed of four to six cycles of about 90-minutes each. Each of these cycles is composed of a succession of several stages of sleep, more or less deep. The analysis of sleep is usually done using a polysomnography. This examination consists of recording, among other things, electrical cerebral activity by electroencephalography (EEG), ocular movements by electrooculography (EOG) and chin muscle tone by electromyography (EMG). The recording is done mostly in a hospital, more specifically in a service for monitoring the pathologies related to sleep. The readings are then interpreted manually by an expert to generate a hypnogram, a curve showing the succession of sleep stages during the night in 30-second epochs. The proposed method is based on the follow-up of the thermal signature that makes it possible to classify the activity into three classes: "awakening", "calm sleep" and "agitated sleep". The contribution of this non-invasive method is part of the screening of sleep disorders, to be validated by a more complete analysis of the sleep. The measure provided by this new system, based on temperature monitoring (patient and ambient), aims to be integrated into the tele-medicine platform developed within the framework of the Smart-EEG project by the SYEL - SYstèmes ELectroniques team. Analysis of the data collected during the first surveys carried out with this method showed a correlation between thermal signature and activity during sleep. The advantage of this method lies in its simplicity and the possibility of carrying out measurements of activity during sleep and without direct contact with the patient at home or hospitals.
Cellular Morphology-Mediated Proliferation and Drug Sensitivity of Breast Cancer Cells
Authors: Domura Ryota , Sasaki Rie , Ishikawa Yuma , Okamoto Masami
Published: May 2017

The interpretation of the local microenvironment of extracellular matrix for malignant tumor cells is in intimate relation with metastatic spread of cancer cells involving the associated issues of cellular proliferation and drug responsiveness. This study was aimed to assess the combination of both surface topographies (fiber alignments) and different stiffness of the polymeric substrates (PLLA and PCL) and collagen substrates (coat and gel) to elucidate the effect of the cellular morphology on cellular proliferation and drug sensitivities of two different types of breast cancer cells (MDA-MB-231 and MCF-7). The morphological spreading parameter of (Nuclear/Cytoplasm) induced by the anthropogenic substrates has correlated intimately with the cellular proliferation and the drug sensitivity (IC50) of cancer cells. This study demonstrated the promising results of the parameter for the evaluation of cancer cell malignancy.
Mechanical Properties and Microstructure Improvement of a Two-Step Strain Induced Melt Activation Processed 7075 Aluminum Alloy Compared to that SIMA Process
Authors: Karamouz Mostafa , Alizadeh Mostafa , Ahmadi Ali Reza
Published: May 2017

Strain induced melt activation process (SIMA) creates a globular microstructure which improves the hardness and ultimate tensile stress (UTS) of 7075 aluminum alloys. But, at the grain boundaries of SIMA processed 7075 aluminum alloy, presence of continuous and brittle intermetallic compounds and the eutectic structure result in decreasing mechanical properties, such as elongation, formability and etc. Hence, in order to improve microstructure and formability of 7075 aluminum alloy respect to SIMA process, a new process was done that is called two-step strain induced melt activation (TSSIMA). This process, which has been mentioned in the experimental procedure section, both organized globular structure and significantly modified the microstructure of the alloy compared to that of the SIMA method. It promoted discontinuity and more homogeneity in distribution of the precipitates and, approximately removed the eutectic mixture. Performing the rolling process on the alloy also revealed that it is more effective in formability enhancement in comparison with SIMA process. Also, it increased the hardness of 7075 aluminum alloy respect to that of SIMA process.
Anderson Insulators in Self-Assembled Gold Nanoparticles Thin Films: Single Electron Hopping between Charge Puddles Originated from Disorder
Authors: Jiang Cheng-Wei , Ni I-Chih , Hsieh Yun-Lian , Tzeng Shien-Der , Wu Cen-Shawn , Kuo Watson
Published: May 2017

The Anderson insulating states in Au nanoparticle assembly are identified and studied under the application of magnetic fields and gate voltages. When the inter-nanoparticle tunneling resistance is smaller than the quantum resistance, the system showing zero Mott gap can be insulating at very low temperature. In contrast to Mott insulators, Anderson insulators exhibit great negative magnetoresistance, inferring charge de-localization in a strong magnetic field. When probed by the electrodes spaced by ~200 nm, they also exhibit interesting gate-modulated current similar to the multi-dot single electron transistors. These results reveal the formation of charge puddles due to interplay of disorder and quantum interference at low temperatures.
Theoretical and Experimental Studies of a Switched Inertance Hydraulic System in a Four-Port High-Speed Switching Valve Configuration  
Authors: Pan Min , Plummer Andrew , El Agha Abdullah
Published: May 2017

The switched inertance hydraulic system (SIHS) is a novel high-bandwidth and energy-efficient digital device which can adjust or control flow and pressure by a means that does not rely on throttling the flow and dissipation of power. An SIHS can provide an efficient step-up or step-down of pressure or flow rate by using a digital control signal. In this article, analytical models of an SIHS in a four-port high-speed switching valve configuration are proposed, and the system dynamics and performance are investigated theoretically and experimentally. The flow responses, system characteristics and power consumption can be predicted effectively and accurately by using the proposed models, which were validated by comparing with experiments and with numerical simulation. The four-port configuration is compared with the three-port configuration, and it is concluded that the former one is less efficient for valves of the same size, but provides a bi-direction control capability. As bi-direction control is a common requirement, this constitutes an important contribution to the development of efficient digital hydraulics.
Quantum Fisher Information of Decohered W and GHZ Superposition States with Arbitrary Relative Phase
Authors: Erol Volkan
Published: May 2017

Quantum Fisher Information (QFI) is a very useful concept for analyzing situations that require phase sensitivity. It become a popular topic especially in Quantum Metrology domain. In this work, we study the changes in quantum Fisher information (QFI) values for one relative arbitrary phased quantum system consisting of a superposition of N Qubits W and GHZ states. In a recent work [7], QFI values of this mentioned system for N qubits were studied. In this work, we extend this problem for the changes of QFI values in some noisy channels for the studied system. We show the changes in QFI depending on noise parameters. We report interesting results for different type of decoherence channels. We show the general case results for this problem.
Lempel-Ziv Complexity of Photonic Quasicrystals
Authors: Monzon Juan J. , Felipe Angel , Sanchez-Soto Luis L.
Published: May 2017

The properties of photonic quasicrystals ultimate rely on their inherent long-range order, a hallmark that can be quantified in many ways depending on the specific aspects to be studied. We use the Lempel-Ziv measure, a basic tool for information theoretic problems, to characterize the complexity of the specific structure under consideration. Using the generalized Fibonacci quasicrystals as our thread, we adress the relation between the optical response and the associated complexity.
Quantifying the National Significance of Local Areas for Regional Conservation Planning: North Carolina’s Mountain Treasures
Authors: Belote R. Travis , Irwin G. Hugh
Published: May 2017

Conservation scientists recognize that additional protected areas are needed to maintain biological diversity and ecological processes. As regional conservation planners embark on recommending additional areas for protection in formal conservation reserves, it is important to evaluate candidate lands for their role in building a resilient protected areas system of the future. Here, we evaluate North Carolina’s Mountain Treasures with respect to their (i) ecological integrity, (ii) role in connecting existing core protected areas, (iii) potential to diversify the ecosystem representation of reserves, and (iv) role in maintaining hotspots of biologically-rich areas not well protected. Mountain Treasures represent a citizen inventory of roadless areas and serve as candidates for elevated levels of conservation protection on U.S. federal lands. We compared Mountain Treasures to other candidate lands throughout the country to evaluate their potential national significance. While the Mountain Treasures tended to be more impacted by human modifications than other roadless areas, they are as important as other roadless areas with respect to their role in connecting existing protected areas and diversifying representation of ecosystems in conservation reserves. However, Mountain Treasures tended to have a much higher biodiversity priority index than other roadless areas leading to an overall higher composite score compared to other roadless areas. Our analysis serves as an example of how using broad-scale datasets can help conservation planners assess the national significance of local areas.
Tratamento cirúrgico de fratura severa de ossos próprios do nariz: relato de caso
Authors: Santos Gabriel Mulinari dos , Pires William Ricardo , Silva Leonardo Freitas , Deus Ciro Borges Duailibe de , Momesso Gustavo Antonio Correa , Polo Tárik Ocon Braga , Souza Francisley Ávila , Garcia Júnior Idelmo Rangel , Faverani Leonardo Perez
Published: May 2017

As fraturas nasais são as fraturas mais frequentes em face, esse fato ocorre devido a proeminência dos ossos próprios do nariz (OPN) em relação a face, que faz com que eles possam receber o primeiro impacto frente a um trauma. As fraturas nasais (FN) podem ter seu tratamento cirúrgico ou não, de acordo com a condição estética e também funcional do paciente. O presente trabalho tem como objetivo relatar um caso clínico de um paciente de 30 anos, sexo masculino, leucoderma, vítima de agressão física, admitido na Santa Casa de Misericórdia de Araçatuba relatando sangramento nasal abundante, dificuldade respiratória e dor em face. Ao exame clínico notou-se rinoescoliose significativa á direita e impermeabilidade das vias aéreas, e no exame de imagem por tomografia computadorizada observou fratura OPN envolvendo fratura e desvio de septo nasal. Pelas condições apresentadas no exame clínico e de imagem, foi proposto tratamento cirúrgico sob anestesia geral. A primeira etapa cirúrgica foi realizada com reposição dos fragmentos, seguida do tamponamento para sustentação com tamponamento, porém sem sucesso estéticos e funcionais. A segunda etapa cirúrgica foi realizada sob anestesia geral, com redução da fratura, do desvio de septo e tamponamento. No pós-operatório foi possível notar um resultado satisfatório funcional com patência das vias aéreas e também estético, quando comparado com imagens fotográficas do paciente prévias ao trauma.Descritores: Fraturas Ósseas; Fixação de Fratura; Traumatismos Faciais.
Habilitação em acupuntura para cirurgiões-dentistas no Brasil: uma análise por estados e regiões
Authors: Fernandes Neto José de Alencar , Silva Maria das Graças Barbosa da , Simões Thamyres Maria Silva , Andrade Flávio de Almeida , Batista Ana Luzia Araújo , Catão Maria Helena Chaves de Vasconcelos
Published: May 2017

Introdução: A prática da acupuntura na Odontologia ainda é pouco utilizada, apesar de ter se mostrado um método terapêutico que agrega benefícios aos tratamentos odontológicos convencionais. Objetivo: avaliar a quantidade de cirurgiões-dentistas habilitados em acupuntura e de cursos da habilitação anteriormente oferecidos, de acordo com os estados e regiões brasileiras. Material e método: Os números totais de cirurgiões-dentistas no Brasil, dos habilitados em acupuntura e as informações sobre os cursos de habilitação já ministrados no país foram coletados diretamente do site do CFO. Todos os dados utilizados nesta pesquisa são de acesso público. Resultados: São Paulo é o estado brasileiro com o maior número de habilitados em acupuntura (n=246), enquanto que nove estados ainda não possuem profissional habilitado nesta prática. O Sudeste detém o maior número de habilitados em acupuntura (n=305), correspondendo a 74,4% do total encontrado em todo o Brasil (n=410). Apenas quatro profissionais (1,0%) estão localizados no Norte, região onde para cada habilitado em acupuntura existem 3.667,8 cirurgiões-dentistas (1/3.667,8). Quanto aos cursos da habilitação oferecidos no país, todos (n=12) foram realizados em dois estados: Paraná (n=8) e São Paulo. Conclusão: Conclui-se que há uma pequena quantidade de cirurgiões-dentistas habilitados e de cursos de habilitação em acupuntura em todo o Brasil, principalmente nas regiões Norte, Nordeste e Centro-Oeste.Descritores: Acupuntura; Terapia por Acupuntura; Odontologia; Odontólogos; Credenciamento; Currículo.
Specific Targeting of HER2-Positive Head and Neck Squamous Cell Carcinoma Line HN5 by Idarubicin-ZHER2 Affibody Conjugate
Authors: Ghanemi Marzieh , Pourshohod Aminollah , Ghaffari Mohammad Ali , kheirollah Alireza , Amin Mansour , Zeinali Majid , Jamalan Mostafa
Published: May 2017

Background:

Expression of human epidermal growth factor receptor type 2 (HER2) in head and neck squamous cell carcinoma (HNSCC) cell line HN5 can be employed with great opportunities of success for specific targeting of anti-cancer chemotherapeutic agents.

Objective:

In the current study, HER2-specific affibody molecule, ZHER2:342 (an engineered protein with great affinity for HER2 receptors) was selected for conjugation to idarubicin (an anti-neoplastic antibiotic).

Method:

ZHER2:342 affibody gene with one added cysteine code at the its 5′ end was synthesized de novo and then inserted into pET302 plasmid and transferred to E. Coli BL21 hosting system. After induction of protein expression, the recombinant ZHER2 affibody molecules were purified using Ni- NTA resin and purity was analyzed through SDS-PAGE. Affinity-purified affibody molecules were conjugated to idarubicin through a heterobifunctional crosslinker, sulfosuccinimidyl 4-(Nmaleimidomethyl) cyclohexane-1-carboxylate (Sulfo-SMCC). Specific toxicity of idarubicin-ZHER2 affibody conjugate against two HER2-positive cells, HN5 and MCF-7 was assessed through MTT assay after an exposure time of 48 hours with different concentrations of conjugate.

Results:

Idarubicin in the non-conjugated form showed potent toxic effects against both cell lines, while HN5 cells were significantly more sensitive compared to MCF-7 cells. Dimeric ZHER2 affibody showed a mild decreasing effect on growth of both HN5 and MCF-7 cells at optimum concentration. Idarubicin-ZHER2 affibody conjugate at an optimum concentration reduced viability of HN5 cell line more efficiently compared to MCF-7 cell line.

In conclusion, idarubicin-ZHER2 affibody conjugate in optimum concentrations can be used for specific targeting and killing of HN5 cells.