bluuebunny/crossref_metadata_2025 huggingface dataset:
Holistic Energy Efficiency and Environmental Friendliness Model for Short-Sea Vessels with Alternative Power Systems Considering Realistic Fuel Pathways and Workloads
Authors: Perčić Maja , Vladimir Nikola , Fan Ailong , Jovanović Ivana
Published: May 2022

Energy requirements push the shipping industry towards more energy-efficient ships, while environmental regulations influence the development of environmentally friendly ships by replacing fossil fuels with alternatives. Current mathematical models for ship energy efficiency, which set the analysis boundaries at the level of the ship power system, are not able to consider alternative fuels as a powering option. In this paper, the energy efficiency and emissions index are formulated for ships with alternative power systems, considering three different impacts on the environment (global warming, acidification, and eutrophication) and realistic fuel pathways and workloads. Besides diesel, applications of alternative powering options such as electricity, methanol, liquefied natural gas, hydrogen, and ammonia are considered. By extending the analysis boundaries from the ship power system to the complete fuel cycle, it is possible to compare different ships within the considered fleet, or a whole shipping sector, from the viewpoint of energy efficiency and environmental friendliness. The applicability of the model is illustrated on the Croatian ro-ro passenger fleet. A technical measure of implementation of alternative fuels in combination with an operational measure of speed reduction results in an even greater emissions reduction and an increase in energy efficiency. Analysis of the impact of voluntary speed reduction for ships with different power systems resulted in the identification of the optimal combination of alternative fuel and speed reduction by a specific percentage from the ship design speed.
Experimental Study on the Probability of Different Wave Impact Types on a Vertical Wall with Horizontal Slab by Separation of Quasi-static Wave Impacts
Authors: Huang Jianjun , Chen Guoping , Lowe Ryan J.
Published: May 2022

When the fundamental natural frequency of marine structures is comparable to the dominant frequency of incident waves, the response of the load on the structure will be amplified. Accurately quantifying how wave loads can be amplified by incident wave conditions must thus be considered in any structural analysis, given how sensitive these characteristics are to different wave impact types. Systematic physical model tests of wave impacts on the simple horizontal plate and the vertical wall with a horizontal overhanging cantilever slab were performed. By first comparing quasi-static wave load estimates along a simple horizontal plate (obtained by low-pass filtering the pressure time series at different cut-off frequencies) with quasi-static uplift pressures from established predictive formulations, a cut-off frequency of 7 Hz was found to accurately separate the quasi-static component from impulsive wave impacts. By applying the low-pass filtering approach with the selected cut-off frequency to the pressure measurements for the vertical wall with a horizontal cantilever slab case, the impulsive and quasi-static peaks were attained, which were then used to quantify the probabilities of individual impulsive, dynamic, and quasi-static wave impacts. Incoming wave conditions and structural clearance had a significant effect on the probabilities of different wave impacts. With the increasing wave height and wave steepness, wave impacts on the horizontal slab and vertical wall were increasingly of the impulsive type and less frequently of the quasi-static type, while the probability of dynamic impact types were relatively stable. As the overhanging slab was shifted from elevated to submerged, the dominant type of wave impact on the structure was variable, ranging from impulsive to dynamic to quasi-static as its elevation was lowered. The results indicated that up to 90% of the impacts were of the impulsive type when the overhanging slab was on or slightly over the still water level. Moreover, the presence of the vertical wall increased the magnitude of wave loads and the occurring frequency of impulsive wave impacts for the horizontal slab.
Resilience Analysis of Maritime Silk Road Shipping Network Structure under Disruption Simulation
Authors: Yang Yanbin , Liu Wei
Published: May 2022

As an important hub in the maritime transportation system, ports are vulnerable to events such as terrorist attacks, security accidents and bad weather. The failure of port nodes to function effectively affects the connectivity and efficiency of the shipping network and impedes trade between countries. In view of this, in this paper, we constructed the Maritime Silk Road shipping network based on route data and used transmissibility and diversity to represent the resilience of the network and nodes. Then, we analyzed the variation characteristics of resilience using disruption simulation and identified 9 dominant nodes and 15 vulnerable nodes that could help to accurately determine the factors that affect the resilience of the MSR shipping network structure. The results show that the Maritime Silk Road shipping network structure is vulnerable, and the failure of ports to function has different effects on network transmissibility and diversity. In terms of node transmissibility and diversity, there are differences in the resistance of port nodes to interventions. In addition, the failure of dominant ports to function and the emergence of vulnerable ports are significant factors that weaken the resilience of the network structure. When dominant ports are interrupted, this greatly affects the resilience of the network structure. It is necessary to reduce the possibilities of the failure of dominant ports. Vulnerable ports are weaknesses in the resilience of the network structure, which weaken the ability of the network to function. The centrality of these ports should be strengthened, and their relation to regional and trans-regional links should be enriched. The research results provide a scientific basis for ensuring the structural resilience of the Maritime Silk Road shipping network.
Efficiency Studies of Fast Neutron Tracking Using MCNP
Authors: Chu Pinghan , James Michael R. , Wang Zhehui
Published: May 2022

Fast neutron identification and spectroscopy is of great interest to nuclear physics experiments. Using the neutron elastic scattering, the fast neutron momentum can be measured. Wang and Morris introduced the theoretical concept that the initial fast neutron momentum can be derived from up to three consecutive elastic collisions between the neutron and the target, including the information of two consecutive recoil ion tracks and the vertex position of the third collision or two consecutive elastic collisions with the timing information. Here, we also include the additional possibility of measuring the deposited energies from the recoil ions. In this paper, we simulate the neutron elastic scattering using the Monte Carlo N-Particle Transport Code (MCNP) and study the corresponding neutron detection and tracking efficiency. The corresponding efficiency and the scattering distances are simulated with different target materials, especially natural silicon (92.23%28Si, 4.67%29Si, and 3.1%30Si) and helium-4 (4He). The timing of collision and the recoil ion energy are also investigated, which are important characters for the detector design. We also calculate the ion traveling range for different energies using the software, “The Stopping and Range of Ions in Matter (SRIM)”, showing that the ion track can be most conveniently observed in 4He unless sub-micron spatial resolution can be obtained in silicon.
Severe Sporotrichosis Treated with Amphotericin B: A 20-Year Cohort Study in an Endemic Area of Zoonotic Transmission
Authors: Fichman Vivian , Freitas Dayvison Francis Saraiva , do Valle Antonio Carlos Francesconi , de Souza Rogerio Valls , Curi André Luiz Land , Valete-Rosalino Cláudia Maria , de Macedo Priscila Marques , Varon Andréa Gina , Figueiredo-Carvalho Maria Helena Galdino , Almeida-Silva Fernando , Zancopé-Oliveira Rosely Maria , Oliveira Raquel de Vasconcelos Carvalhaes , Almeida-Paes Rodrigo , Gutierrez-Galhardo Maria Clara
Published: May 2022

Although rare, disseminated sporotrichosis is increasing in several countries. Despite its limiting toxic potential, amphotericin B is the only intravenous antifungal available to treat severe sporotrichosis. We aimed to describe the effectiveness and safety of amphotericin B treatment for severe sporotrichosis. Clinical records of patients with disseminated sporotrichosis at a reference center were reviewed. This study included 73 patients. Most (53.4%) were men and non-white. HIV coinfection was the main comorbidity (52.1%). Most reported contact with cats (76.7%). Sporothrix brasiliensis was the causative species. Affected sites were skin (98.6%), osteoarticular system (64.4%), upper airway (42.5%), central nervous system (20.5%), eyes (12.3%), and lungs (8.2%). Median doses of amphotericin B used were 750 mg and 4500 mg for deoxycholate and lipid complex formulations, respectively. Amphotericin B discontinuation occurred in 20.5% due to adverse events, mainly azotemia. The outcomes included cure (52.1%), death due to sporotrichosis (21.9%), death due to other causes (9.6%), and loss to follow-up (8.2%). Survival analysis showed an association between cure and the absence of bone, upper airway, and central nervous system involvement. Amphotericin B is the first-choice treatment for disseminated sporotrichosis; however, the severity of systemic dissemination might predict its response. Favorable clinical results depend on prompt diagnosis, investigation of fungal dissemination, and early therapy initiation.
Squalene Monooxygenase Gene SsCI80130 Regulates Sporisorium scitamineum Mating/Filamentation and Pathogenicity
Authors: Cai Yichang , Zhang Yi , Bao Han , Chen Jiaoyun , Chen Jianwen , Shen Wankuan
Published: May 2022

Sugarcane is an important sugar crop and energy crop worldwide. Sugarcane smut caused by Sporisorium scitamineum is a serious fungal disease that occurs worldwide, seriously affecting the yield and quality of sugarcane. It is essential to reveal the molecular pathogenesis of S. scitamineum to explore a new control strategy of sugarcane smut. Based on transcriptome sequencing data of two S. scitamineum strains Ss16 and Ss47, each with a different pathogenicity, our laboratory screened out the SsCI80130 gene predicted to encode squalene monooxygenase. In this study, we obtained the knockout mutants (ΔSs80130+ and ΔSs80130−) and complementary mutants (COM80130+ and COM80130−) of this gene by the polyethylene glycol-mediated (PEG-mediated) protoplast transformation technology, and then performed a functional analysis of the gene. The results showed that the deletion of the SsCI80130 gene resulted in the increased content of squalene (substrate for squalene monooxygenase) and decreased content of ergosterol (the final product of the ergosterol synthesis pathway) in S. scitamineum. Meanwhile, the sporidial growth rate of the knockout mutants was significantly slower than that of the wild type and complementary mutants; under cell-wall stress or oxidative stress, the growth of the knockout mutants was significantly inhibited. In addition, the sexual mating ability and pathogenicity of knockout mutants were significantly weakened, while the sexual mating ability could be restored by adding exogenous small-molecular signal substance cAMP (cyclic adenosine monophosphate) or tryptophol. It is speculated that the SsCI80130 gene was involved in the ergosterol biosynthesis in S. scitamineum and played an important role in the sporidial growth, stress response to different abiotic stresses (including cell wall stress and oxidative stress), sexual mating/filamentation and pathogenicity. Moreover, the SsCI80130 gene may affect the sexual mating and pathogenicity of S. scitamineum by regulating the ergosterol synthesis and the synthesis of the small-molecular signal substance cAMP or tryptophol required for sexual mating. This study reveals for the first time that the gene encoding squalene monooxygenase is involved in regulating the sexual mating and pathogenicity of S. scitamineum, providing a basis for the molecular pathogenic mechanism of S. scitamineum.
Highly Diverse Phytophthora infestans Populations Infecting Potato Crops in Pskov Region, North-West Russia
Authors: Runno-Paurson Eve , Agho Collins A. , Zoteyeva Nadezda , Koppel Mati , Hansen Merili , Hallikma Tiit , Cooke David E. L. , Nassar Helina , Niinemets Ülo
Published: May 2022

There is limited understanding of the genetic variability in Phytophthora infestans in the major potato cultivation region of north-western Russia, where potato is grown primarily by small households with limited chemical treatment of late blight. In this study, the mating type, sensitivity to metalaxyl, and genotype and population genetic diversity (based on 12 simple sequence repeat (SSR) markers) of 238 isolates of P. infestans from the Pskov region during the years 2010–2013 were characterized. The aim was to examine the population structure, phenotypic and genotypic diversity, and the prevalent reproductive mode of P. infestans, as well as the influence of the location, time, and agricultural management practices on the pathogen population. The frequency of the A2 mating was stable over the four seasons and ranged from 33 to 48% of the sampled population. Both mating types occurred simultaneously in 90% of studied fields, suggesting the presence of sexual reproduction and oospore production in P. infestans in the Pskov region. Metalaxyl-sensitive isolates prevailed in all four years (72%), however, significantly fewer sensitive isolates were found in samples from large-scale conventional fields. A total of 50 alleles were detected in the 141 P. infestans isolates analyzed for genetic diversity. Amongst the 83 SSR multilocus genotypes (MLGs) detected, 65% were unique and the number of MLGs varied between locations from 3 to 20. These results, together with the high genotypic diversity observed in all the locations and the lack of significance of linkage disequilibrium, suggest that sexual recombination is likely responsible for the unique MLGs and the high genetic diversity found in the Pskov region population, resembling those of north-eastern European populations.
Postharvest Rot of Pomegranate Fruit in Southern Italy: Characterization of the Main Pathogens
Authors: Mincuzzi Annamaria , Sanzani Simona Marianna , Palou Lluís , Ragni Marco , Ippolito Antonio
Published: May 2022

Pomegranate (Punica granatum L.) is an emerging crop in Italy and particularly in southern regions, such as Apulia, Basilicata, and Sicily, due to favorable climatic conditions. The crop is affected by several pathogenic fungi, primarily in the field, but also during postharvest phases. The most important postharvest fungal diseases in pomegranate are gray and blue molds, black heart and black spot, anthracnose, dry rot, and various soft rots. The limited number of fungicides allowed for treatment in the field and the lack of postharvest fungicides make it difficult to control latent, quiescent, and incipient fungal infections. Symptomatic pomegranates from southern Italy were sampled and isolated fungi were morphologically and molecularly characterized. The data obtained revealed that various species of Penicillium sensu lato (including Talaromyces genus), Alternaria spp., Coniella granati, and Botrytis cinerea were the principal etiological agents of postharvest pomegranate fruit diseases; other relevant pathogens, although less represented, were ascribable to Aspergillus sect. nigri, Colletotrichum acutatum sensu stricto, and Cytospora punicae. About two thirds of the isolated pathogens were responsible for latent infections. The results obtained may be useful in planning phytosanitary control strategies from the field to storage, so as to reduce yield losses.
The Category of Conventional Physiotherapy: The Case of Parkinson’s Disease Guidelines
Authors: Hoskovcová Martina , Růžička Evžen , Gál Ota ,   on behalf of the iCARE-PD Consortium
Published: May 2022

This opinion paper reviews the use of the category of “conventional physiotherapy” (CPT) in Parkinson’s disease (PD)-relevant reviews and meta-analyses and points out serious inconsistencies within and among them. These are first discussed in general, leading to the conclusion that, in most cases, the category of CPT encompasses a range of incompatible interventions. This undermines previous conclusions about their superiority or inferiority relative to various other treatment modalities. Next, the update to the European Physiotherapy Guidelines is discussed in detail, since it treats CPT as a global and time-independent category per se, ascribing effects in various domains to it. This introduces several important biases into the findings presented in this publication. These are subsequently discussed, and it is concluded that the categorisation of various physiotherapy techniques under the umbrella term of CPT is empty, or even dangerous, and should be abandoned. Other categories are suggested as a replacement, including “Other Physiotherapy Techniques” and “Multimodal Training”.
Efficacy of Cryoballoon Ablation for Atrial Fibrillation and Recurrence Predictors in an Asian Cohort
Authors: Wu Shang-Ju , Li Cheng-Hung , Weng Chi-Jen , Lin Jiunn-Cherng , Chien Yu-Shan , Chen Yi-Huei , Lin Ching-Heng , Hsieh Yu-Cheng , Huang Jin-Long , Lo Li-Wei , Lin Yenn-Jiang , Chen Shih-Ann
Published: May 2022

Background: Cryoballoon ablation (CBA) for atrial fibrillation (AF) is a rhythm control procedure used in clinical trials, mostly in Western countries. Its efficacy and the predictors of AF recurrence after CBA remain unclear for Asian populations. We aimed to investigate the efficacy of CBA and the predictors of AF recurrence after CBA in Asian AF patients. Methods: We included consecutive AF patients undergoing CBA for rhythm control between 2014 and 2020. The baseline characteristics, including AF types, symptom severity, and left atrial diameter (LAD), were analyzed. Holter’s monitoring and 12-lead ECG were performed to document AF recurrence. A multivariate Cox hazards regression model was used to evaluate the risk of AF recurrence. Results: A total of 120 AF patients (aged 61.9 ± 9.3 years) were included. The percentage of patients free from AF in the year following CBA was 74.2%. Among the three independent predictors of AF recurrence within one year were the presence of persistent AF (p = 0.025), an LAD ≥ 4.75 cm (p = 0.016), and pre-procedural cardioversion (p = 0.025). All patients survived and none had a stroke after CBA. Conclusion: CBA for AF is an effective and safe procedure in Asian populations. The presence of persistent AF, an LAD ≥ 4.75 cm, and severe symptoms are predictors of AF recurrence in the year following CBA.
Signal-to-Noise Analysis Can Inform the Likelihood That Incidentally Identified Variants in Sarcomeric Genes Are Associated with Pediatric Cardiomyopathy
Authors: Kurzlechner Leonie M. , Jones Edward G. , Berkman Amy M. , Tadros Hanna J. , Rosenfeld Jill A. , Yang Yaping , Tunuguntla Hari , Allen Hugh D. , Kim Jeffrey J. , Landstrom Andrew P.
Published: May 2022

Background: Hypertrophic cardiomyopathy (HCM) is the most common heritable cardiomyopathy and can predispose individuals to sudden death. Most pediatric HCM patients host a known pathogenic variant in a sarcomeric gene. With the increase in exome sequencing (ES) in clinical settings, incidental variants in HCM-associated genes are being identified more frequently. Diagnostic interpretation of incidental variants is crucial to enhance clinical patient management. We sought to use amino acid-level signal-to-noise (S:N) analysis to establish pathogenic hotspots in sarcomeric HCM-associated genes as well as to refine the 2015 American College of Medical Genetics (ACMG) criteria to predict incidental variant pathogenicity. Methods and Results: Incidental variants in HCM genes (MYBPC3, MYH7, MYL2, MYL3, ACTC1, TPM1, TNNT2, TNNI3, and TNNC1) were obtained from a clinical ES referral database (Baylor Genetics) and compared to rare population variants (gnomAD) and variants from HCM literature cohort studies. A subset of the ES cohort was clinically evaluated at Texas Children’s Hospital. We compared the frequency of ES and HCM variants at specific amino acid locations in coding regions to rare variants (MAF < 0.0001) in gnomAD. S:N ratios were calculated at the gene- and amino acid-level to identify pathogenic hotspots. ES cohort variants were re-classified using ACMG criteria with S:N analysis as a correlate for PM1 criteria, which reduced the burden of variants of uncertain significance. In the clinical validation cohort, the majority of probands with cardiomyopathy or family history hosted likely pathogenic or pathogenic variants. Conclusions: Incidental variants in HCM-associated genes were common among clinical ES referrals, although the majority were not disease-associated. Leveraging amino acid-level S:N as a clinical tool may improve the diagnostic discriminatory ability of ACMG criteria by identifying pathogenic hotspots.
Goal-Directed Fluid Therapy Enhances Gastrointestinal Recovery after Laparoscopic Surgery: A Systematic Review and Meta-Analysis
Authors: Virág Marcell , Rottler Máté , Gede Noémi , Ocskay Klementina , Leiner Tamás , Tuba Máté , Ábrahám Szabolcs , Farkas Nelli , Hegyi Péter , Molnár Zsolt
Published: May 2022

(1) Background: Whether goal-directed fluid therapy (GDFT) provides any outcome benefit as compared to non-goal-directed fluid therapy (N-GDFT) in elective abdominal laparoscopic surgery has not been determined yet. (2) Methods: A systematic literature search was conducted in MEDLINE, Embase, CENTRAL, Web of Science, and Scopus. The main outcomes were length of hospital stay (LOHS), time to first flatus and stool, intraoperative fluid and vasopressor requirements, serum lactate levels, and urinary output. Pooled risks ratios (RRs) with 95% confidence intervals (CI) were calculated for dichotomous outcomes and weighted mean difference (WMD) with 95% CI for continuous outcomes. (3) Results: Eleven studies were included in the quantitative, and fifteen in the qualitative synthesis. LOHS (WMD: −1.18 days, 95% CI: −1.84 to −0.53) and time to first stool (WMD: −9.8 h; CI −12.7 to −7.0) were significantly shorter in the GDFT group. GDFT resulted in significantly less intraoperative fluid administration (WMD: −441 mL, 95% CI: −790 to −92) and lower lactate levels at the end of the operation: WMD: −0.25 mmol L−1; 95% CI: −0.36 to −0.14. (4) Conclusions: GDFT resulted in enhanced recovery of the gastrointestinal function and shorter LOHS as compared to N-GDFT.
Initiation Timing of Continuous Interscalene Brachial Plexus Blocks in Patients Undergoing Shoulder Arthroplasty: A Retrospective Before-and-After Study
Authors: Kim Ha-Jung , Kim Hyojune , Koh Kyoung Hwan , Jeon In-Ho , Kim Hyungtae , Ro Young-Jin , Koh Won Uk
Published: May 2022

A continuous interscalene brachial plexus block (CIBPB) is usually administered before surgery in awake patients. However, the use of CIBPB before surgery could hinder the identification of nerve injuries after total shoulder arthroplasty (TSA). This study aimed to compare the analgesic effects of preoperatively and postoperatively initiated CIBPBs in patients undergoing TSA. The medical records of patients who underwent TSA between January 2016 and August 2020 were retrospectively reviewed. The following analgesic phases were used: intravenous (IV) patient-controlled analgesia (PCA) phase (IV PCA group, n = 40), preoperative block phase (PreBlock group, n = 44), and postoperative block phase (PostBlock group, n = 33). The postoperative initiation of CIBPB after a neurologic exam provided better analgesia than IV PCA and had no differences with the preoperative initiation of CIBPB, except for the worst pain at the postanesthetic care unit. Opioid consumption was significantly greater in the IV PCA group, but there were no differences between the PreBlock and PostBlock groups on operation day after the transfer to the general ward. The initiation of CIBPB after a patient’s emergence from general anesthesia had comparable analgesic efficacy with preoperative CIBPB but offered the chance of a postoperative neurologic exam.
An Investigation of the Link between Major Shareholders’ Behavior and Corporate Governance Performance before and after the COVID-19 Pandemic: A Case Study of the Companies Listed on the Iranian Stock Market
Authors: Pourmansouri Rezvan , Mehdiabadi Amir , Shahabi Vahid , Spulbar Cristi , Birau Ramona
Published: May 2022

One of the basic functions of establishing corporate governance (CG) in companies is improving performance and increasing value for shareholders. Expanding the company’s value will ultimately increase the shareholders’ wealth. Therefore, it is natural for shareholders to seek to improve their performance and increase the company’s value. If CG mechanisms cannot perform this function in companies, they do not have the necessary efficiency and effectiveness and, therefore, cannot improve the efficiency of companies. This article investigated the connection between the power of major shareholders and the modality of CG of companies listed on the Iranian capital market before and after the COVID-19 pandemic. The statistical sample of the research included 120 companies listed on the Tehran Stock Exchange for the selected period from 2011 to 2021. The results showed that the concentration of ownership is harmful to adopting corporate governance (GCG) practices. In particular, the high level of voter ownership concentration weakens the corporate governance system (CGS). The results of this study, which was conducted using panel analysis, revealed that the concentration of ownership impairs the quality of CGS, and major shareholders cannot challenge the power of the main shareholder; it alsonegatively affected the quality of business boards, both during and before the COVID-19 pandemic. The competitiveness and voting rights of the major shareholders negatively affected the quality of board composition before and after the COVID-19 pandemic. The concentration of voter ownership also negatively affected the quality of CGS, both during and before COVID-19, and the competitiveness and voting rights of major shareholders before COVID-19. This concentration positively affected the quality of CGS after the COVID-19 pandemic.
Spatiotemporal Dynamics of Grasslands Using Landsat Data in Livestock Micro-Watersheds in Amazonas (NW Peru)
Authors: Marin Nilton Atalaya , Barboza Elgar , López Rolando Salas , Vásquez Héctor V. , Gómez Fernández Darwin , Terrones Murga Renzo E. , Rojas Briceño Nilton B. , Oliva-Cruz Manuel , Gamarra Torres Oscar Andrés , Silva López Jhonsy O. , Cayo Efrain Turpo
Published: May 2022

In Peru, grasslands monitoring is essential to support public policies related to the identification, recovery and management of livestock systems. In this study, therefore, we evaluated the spatial dynamics of grasslands in Pomacochas and Ventilla micro-watersheds (Amazonas, NW Peru). To do this, we used Landsat 5, 7 and 8 images and vegetation indices (normalized difference vegetation index (NDVI), enhanced vegetation index (EVI) and soil adjusted vegetation index (SAVI). The data were processed in Google Earth Engine (GEE) platform for 1990, 2000, 2010 and 2020 through random forest (RF) classification reaching accuracies above 85%. The application of RF in GEE allowed surface mapping of grasslands with pressures higher than 85%. Interestingly, our results reported the increase of grasslands in both Pomacochas (from 2457.03 ha to 3659.37 ha) and Ventilla (from 1932.38 ha to 4056.26 ha) micro-watersheds during 1990–2020. Effectively, this study aims to provide useful information for territorial planning with potential replicability for other cattle-raising regions of the country. It could further be used to improve grassland management and promote semi-extensive livestock farming.
Integration of Three-Dimensional Liver Models in a Multimodal Image-Guided Robotic Liver Surgery Cockpit
Authors: Bijlstra Okker D. , Broersen Alexander , Oosterveer Timo T. M. , Faber Robin A. , Achterberg Friso B. , Hurks Rob , Burgmans Mark C. , Dijkstra Jouke , Mieog J. Sven D. , Vahrmeijer Alexander L. , Swijnenburg Rutger-Jan
Published: May 2022

Background: Robotic liver surgery represents the most recent evolution in the field of minimally-invasive liver surgery. For planning and guidance of liver resections, surgeons currently rely on preoperative 2-dimensional (2D) CT and/or MR imaging and intraoperative ultrasonography. Translating 2D images into digital 3-dimensional (3D) models may improve both preoperative planning and surgical guidance. The da Vinci® robotic surgical system is a platform suitable for the integration of multiple imaging modalities into one single view. In this study, we describe multimodal imaging options and introduce the Robotic Liver Surgery Cockpit; Methods: in-house developed software was used and validated for segmentation and registration to create a virtual reality 3D model of the liver based on preoperative imaging. The accuracy of the 3D models in the clinical setting was objectively assessed in 15 patients by measuring tumor diameters and subjectively with a postoperative conducted questionnaire; Results: Implementation and applicability of the 3D model in the surgical cockpit was feasible in all patients and the quality of the 3D reconstructions was high in 14 (93%) of cases. Tumor diameters measured on CT and/or MR imaging were comparable to automated measurements using the segmentation software and 3D models; Conclusions: the 3D model was successfully incorporated in the robotic surgery console as part of a multimodality imaging platform and aided the surgeon in planning and guidance of the resection. Future studies should focus on further automation of 3D rendering and progress into augmented reality.
Systems-Based Approach for Optimization of Assembly-Free Bacterial MLST Mapping
Authors: Pavlovikj Natasha , Gomes-Neto Joao Carlos , Deogun Jitender S. , Benson Andrew K.
Published: May 2022

Epidemiological surveillance of bacterial pathogens requires real-time data analysis with a fast turnaround, while aiming at generating two main outcomes: (1) species-level identification and (2) variant mapping at different levels of genotypic resolution for population-based tracking and surveillance, in addition to predicting traits such as antimicrobial resistance (AMR). Multi-locus sequence typing (MLST) aids this process by identifying sequence types (ST) based on seven ubiquitous genome-scattered loci. In this paper, we selected one assembly-dependent and one assembly-free method for ST mapping and applied them with the default settings and ST schemes they are distributed with, and systematically assessed their accuracy and scalability across a wide array of phylogenetically divergent Public Health-relevant bacterial pathogens with available MLST databases. Our data show that the optimal k-mer length for stringMLST is species-specific and that genome-intrinsic and -extrinsic features can affect the performance and accuracy of the program. Although suitable parameters could be identified for most organisms, there were instances where this program may not be directly deployable in its current format. Next, we integrated stringMLST into our freely available and scalable hierarchical-based population genomics platform, ProkEvo, and further demonstrated how the implementation facilitates automated, reproducible bacterial population analysis.
Characteristics of the Cardiosplenic Axis in Patients with Fatal Myocardial Infarction
Authors: Kercheva Maria , Ryabov Vyacheslav , Trusov Andrey , Stepanov Ivan , Kzhyshkowska Julia
Published: May 2022

Myocardial ischemia triggers neurohumoral activation of the cardiosplenic axis. In rodents, adverse outcomes occur upon prolonged entrance of mononuclear cells from the spleen into myocardial tissue. The purpose of this study is to assess the features of spleen structure in patients with fatal myocardial infarction (MI), the dynamics of macrophage infiltration of the spleen and its relationship with cardiac macrophage infiltration and unfavorable outcomes. Using immunohistochemistry techniques, we analyzed the macrophage infiltration of the spleen and myocardium sections collected from patients (n = 30) with fatal MI. The spleen of the patients was decreased and showed a predominance of red pulp with a high concentration of CD68+ and stabilin-1+ cells. The white pulp contained many medium and small follicles and a lower concentration of CD68+ and stabilin-1+ cells, which was comparable to that in the infarct area of the myocardium. The concentration of CD68+ and stabilin-1+ cells increased in the myocardium in the late period of MI, but did not show any dynamics in the spleen. A high number of CD68+ cells in the red pulp and reduced concentration of stabilin-1+ cells in the white pulp were associated with unfavorable post-infarction outcomes. These fundamental findings could be a basis for the development of new personalized therapeutic and diagnostic approaches for the treatment of MI and its complications.
Apocynin Attenuates Diabetes-Induced Skeletal Muscle Dysfunction by Mitigating ROS Generation and Boosting Antioxidant Defenses in Fast-Twitch and Slow-Twitch Muscles
Authors: Sánchez-Duarte Sarai , Montoya-Pérez Rocío , Márquez-Gamiño Sergio , Vera-Delgado Karla S. , Caudillo-Cisneros Cipriana , Sotelo-Barroso Fernando , Sánchez-Briones Luis A. , Sánchez-Duarte Elizabeth
Published: May 2022

In response to diabetes mellitus, skeletal muscle is negatively affected, as is evident by reduced contractile force production, increased muscle fatigability, and increased levels of oxidative stress biomarkers. Apocynin is a widely used NADPH oxidase inhibitor, with antioxidant and anti-inflammatory potential. It has been effective for amelioration of a variety of disorders, including diabetic complications. Therefore, the present study was conducted to evaluate the effects and action mechanisms of apocynin in slow- and fast-twitch diabetic rat muscles. Male Wistar rats were rendered diabetic by applying intraperitoneally a single dose of streptozotocin (45 mg/kg). Apocynin treatment (3 mg/kg/day) was administered over 8 weeks. Fasting blood glucose (FBG), insulin tolerance and body weight gain were measured. Both slow (soleus) and fast (extensor digitorum longus, EDL) skeletal muscles were used for muscle function evaluation, oxidative stress markers, and evaluating gene expression using qRT-PCR. Treatment with apocynin significantly reduced FBG levels and enhanced insulin tolerance. Apocynin also prevented muscle contractile dysfunction in EDL muscle but had no significant effect on this parameter in soleus muscles. However, in both types of muscles, apocynin mitigated the oxidative stress by decreasing ROS levels and increasing total glutathione levels and redox state. Concomitantly, apocynin also statistically enhanced Nrf-2 and GLU4 mRNA expression and downregulated NOX2, NOX4, and NF-κB mRNA. Collectively, apocynin exhibits properties myoprotective in diabetic animals. These findings indicate that apocynin predominantly acts as an antioxidant in fast-twitch and slow-twitch muscles but has differential impact on contractile function.
Relating Dry Friction to Interdigitation of Surface Passivation Species: A Molecular Dynamics Study on Amorphous Carbon
Authors: Falk Kerstin , Reichenbach Thomas , Gkagkas Konstantinos , Moseler Michael , Moras Gianpietro
Published: May 2022

Friction in boundary lubrication is strongly influenced by the atomic structure of the sliding surfaces. In this work, friction between dry amorphous carbon (a-C) surfaces with chemisorbed fragments of lubricant molecules is investigated employing molecular dynamic simulations. The influence of length, grafting density and polarity of the fragments on the shear stress is studied for linear alkanes and alcohols. We find that the shear stress of chain-passivated a-C surfaces is independent of the a-C density. Among all considered chain-passivated systems, those with a high density of chains of equal length exhibit the lowest shear stress. However, shear stress in chain-passivated a-C is consistently higher than in a-C surfaces with atomic passivation. Finally, surface passivation species with OH head groups generally lead to higher friction than their non-polar analogs. Beyond these qualitative trends, the shear stress behavior for all atomic- and chain-passivated, non-polar systems can be explained semi-quantitatively by steric interactions between the two surfaces that cause resistance to the sliding motion. For polar passivation species electrostatic interactions play an additional role. A corresponding descriptor that properly captures the interlocking of the two surfaces along the sliding direction is developed based on the maximum overlap between atoms of the two contacting surfaces.
Cu(C3H3N3S3)3 Adsorption onto ZnTiO3/TiO2 for Coordination-Complex Sensitized Photochemical Applications
Authors: Jaramillo-Fierro Ximena , Hernández Karol , González Silvia
Published: May 2022

Currently, the design of highly efficient materials for photochemical applications remains a challenge. In this study, an efficient semiconductor was prepared, based on a coordination complex (Cu-TTC) of Cu(I) and trithiocyanuric acid on ZnTiO3/TiO2 (ZTO/TO). The Cu-TTC/ZTO/TO composite was prepared by the solvothermal method at room temperature. The structural, optical, and electrochemical characteristics, as well as the photocatalytic performance of the composite, were experimentally and computationally studied. The results show that the Cu-TTC/ZTO/TO composite efficiently extended its photoresponse in the visible region of the electromagnetic spectrum. The electrochemistry of the proposed tautomeric architecture (s-Cu-TTC) clearly reveals the presence of metal–ligand charge-transfer (MLCT) and π → π* excitations. The maximum methylene blue (MB) dye photodegradation efficiency of 95% in aqueous solutions was achieved under the illumination of simulated solar light. Finally, computational calculations based on the Density Functional Theory (DFT) method were performed to determine the electronic properties of the s-Cu-TTC tautomeric structure and clarify the adsorption mechanism of this complex on the surface (101) of both ZnTiO3 and TiO2 oxides. The results obtained allow us to suggest that the Cu-TTC complex is an effective charge carrier and that the Cu-TTC/ZTO/TO composite can be used efficiently for photochemical applications.
Snail Based Carbonated-Hydroxyapatite Material as Adsorbents for Water Iron (II)
Authors: Asimeng Bernard Owusu , Amenyaglo Edward Kwame , Dodoo-Arhin David , Efavi Johnson Kwame , Kwakye-Awuah Bright , Tiburu Elvis Kwason , Foster E. Johan , Czernuska Jan
Published: May 2022

Carbonated hydroxyapatite (CHAp) adsorbent material was prepared from Achatina achatina snail shells and phosphate-containing solution using a wet chemical deposition method. The CHAp adsorbent material was investigated to adsorb aqua Fe(II) complex; [Fe(H2O)6]2+ from simulated iron contaminated water for potential iron remediation application. The CHAp was characterized before and after adsorption using infrared (IR) and Raman spectroscopy. The IR and the Raman data revealed that the carbonate functional groups of the CHAp adsorbent material through asymmetric orientation in water bonded strongly to the aqua Fe(II) complex adsorbate. The adsorption behaviour of the adsorbate onto the CHAp adsorbent correlated well to pseudo-second-order kinetics model, non-linear Langmuir and Freundlich model at room temperature of a concentration (20–100 mg L−1) and contact time of 180 min. The Langmuir model estimated the maximum adsorption capacity to be 45.87 mg g−1 whereas Freundlich model indicated an S-type isotherm curvature which supported the spectroscopy revelation.
Experimental and Numerical Investigations on the Damage Induced in the Shearing Process for QP980 Steel
Authors: Han Shuo , Chang Ying , Wang Cunyu , Han Yun , Dong Han
Published: May 2022

Ultra-high-strength quenching and partitioning (Q&P) steels have achieved remarkable lightweight effect in automotive manufacture due to the excellent mechanical performances. However, the problem of sheared-edge cracking greatly limits their application. In this work, the damage generated in the shearing process of QP980 steel is experimentally investigated via microstructure characterization and micro-/macromechanical property evaluation. Moreover, the shearing deformation is simulated with six widely used damage models. The experimental results reveal that microvoids, microcracks, and work-hardening behavior are the main damage factors affecting the formability of sheared edges. Microvoids mainly formed at phase interfaces have a small size (≤5 μm), while microvoids generated from inclusions with a small number have a large size (>5 μm). As deformation continuously grows, microvoids distributed around the sheared surface are split into microcracks, which act as crack initiators in the subsequent forming step. Additionally, the highest microhardness in the fracture zone further enhances the susceptibility of edge cracking. Furthermore, the optimum damage model for QP980 steel was determined by developing user-defined subroutine VUSDFLD in Abaqus, which can be used in the prediction of fracture behavior of QP980 steel to reduce the risk of edge cracking.
Accelerated Aging of Epoxy Biocomposites Filled with Cellulose
Authors: Busiak Radosław , Masek Anna , Węgier Aleksandra , Rylski Adam
Published: May 2022

The presented research concerns the mechanochemical modification of a snap-cure type of epoxy resin, A.S. SET 1010, with the addition of different amounts of cellulose (0, 2, 5, 10, 15 and 20 per 100 resin), for a novel, controlled-degradation material with possible application in the production of passenger seats in rail transport. Composite samples were prepared on a hydraulic press in ac-cordance with the resin manufacturer’s recommendations, in the form of tiles with dimensions of 80 × 80 × 1 mm. The prepared samples were subjected to thermo-oxidative aging and weathering for a period of 336 h. Changes in the color and surface defects in the investigated composites were evaluated using UV-Vis spectrophotometry (Cie-Lab). The degree of degradation by changes in the chemical structure of the samples was analyzed using FTIR/ATR spectroscopy. Differential scan-ning calorimetry (DSC) and thermogravimetric analysis (TGA) tests were performed, and the sur-face energy of the samples was determined by measuring the contact angle of droplets. Tests were performed to determine changes in cellulose-filled epoxy resin composites after thermo-oxidative aging and weathering. It was found out that the addition of cellulose did not inflict sufficient changes to the properties within tested parameters. In the tested case, cellulose acted as a natural active biofiller. Our research is in line with the widespread pursuit of pro-ecological solutions in industry and the creation of materials with a positive impact on the natural environment.
The Auto-Combustion Method Synthesized Eu2O3- ZnO Nanostructured Composites for Electronic and Photocatalytic Applications
Authors: AlAbdulaal Thekrayat H. , Ganesh Vanga , AlShadidi Manal , Hussien Mai S. A. , Bouzidi Abdelfatteh , Algarni Hamed , Zahran Heba Y. , Abdel-wahab Mohamed Sh. , Yahia Ibrahim S. , Nasr Samia
Published: May 2022

An efficient and environmentally friendly combustion technique was employed to produce ZnO nanopowders with different Eu concentrations (from 0.001 g to 5 g). The structural morphology of the Eu2O3-ZnO nanocomposites was examined using XRD, SEM, and infrared spectroscopy (FT-IR). In addition, UV-Vis diffuse reflectance spectroscopy was also used to investigate the effects of europium (Eu) dopant on the optical behaviors and energy bandgaps of nano-complex oxides. The photocatalytic degradation efficiency of phenol and methylene blue was investigated using all the prepared Eu2O3-ZnO nanostructured samples. Photocatalytic effectiveness increased when europium (Eu) doping ratios increased. After adding moderate Eu, more hydroxyl radicals were generated over ZnO. The best photocatalyst for phenol degradation was 1 percent Eu2O3-ZnO, while it was 0.5 percent Eu2O3-ZnO for methylene blue solutions. The obtained Eu2O3-doped ZnO nanostructured materials are considered innovative, promising candidates for a wide range of nano-applications, including biomedical and photocatalytic degradation of organic dyes and phenol.
The Effect of the Heating Rate during Carbonization on the Porosity, Strength, and Electrical Resistivity of Graphite Blocks Using Phenolic Resin as a Binder
Authors: Lee Sang-Hye , Kim Jae-Hyun , Kim Woo-Seok , Roh Jae-Seung
Published: May 2022

In the present study, graphite blocks were fabricated using synthetic graphite scrap and phenolic resin, and the effect of the heating rate during carbonization on their mechanical and electrical characteristics was examined. While varying the heating rate from 1, 3, 5, and 7 to 9 °C/min, the microstructure, density, porosity, flexural strength, compressive strength, and electrical resistivity of the fabricated graphite blocks were measured. As the heating rate increased, the pores in the graphite blocks increased in size, and the shape of the gas release paths became more irregular. Overall, it was found that increases in the heating rate led to the degradation of the graphite blocks’ mechanical and electrical properties.
Investigation of the Fabrication Suitability, Structural Performance, and Sustainability of Natural Fibers in Coreless Filament Winding
Authors: Mindermann Pascal , Gil Pérez Marta , Knippers Jan , Gresser Götz T.
Published: May 2022

Coreless filament winding is an emerging fabrication technology in the field of building construction with the potential to significantly decrease construction material consumption, while being fully automatable. Therefore, this technology could offer a solution to the increasing worldwide demand for building floor space in the next decades by optimizing and reducing the material usage. Current research focuses mainly on the design and engineering aspects while using carbon and glass fibers with epoxy resin; however, in order to move towards more sustainable structures, other fiber and resin material systems should also be assessed. This study integrates a selection of potential alternative fibers into the coreless filament winding process by adapting the fabrication equipment and process. A bio-based epoxy resin was introduced and compared to a conventional petroleum-based one. Generic coreless wound components were created for evaluating the fabrication suitability of selected alternative fibers. Four-point bending tests were performed for assessing the structural performance in relation to the sustainability of twelve alternative fibers and two resins. In this study, embodied energy and global warming potential from the literature were used as life-cycle assessment indexes to compare the material systems. Among the investigated fibers, flax showed the highest potential while bio-based resins are advisable at low fiber volume ratios.