bluuebunny/crossref_metadata_2025 huggingface dataset:
Differences in Self-Concept and Its Dimensions in Students of the Third Cycle of Primary School, Obligatory Secondary Education, and Baccalaureate
Authors: Denche-Zamorano Angel , Mayordomo-Pinilla Noelia , Galán-Arroyo Carmen , Mañanas-Iglesias Carlos , Adsuar Jose C. , Rojo-Ramos Jorge
Published: March 2023

Self-concept can be defined as a structured, multidimensional, and evolving construct that constitutes all the beliefs that an individual has about him/herself. Among its dimensions is the physical dimension that encompasses perceptions of physical attractiveness, self-esteem, and physical condition. The purpose of this cross-sectional study was to look for differences between the educational stages from the third cycle of primary school and high school, as well as to study the possible correlations between the age groups and the dimensions of the scale. The AF-5 scale was used to measure self-concept, the Kolmogoronov–Smirnov test was applied to determine the normality of the data, Kruskall–Wallis to identify the differences between the dimensions of the scale and the educational stages, and Spearman’s Rho for correlations between dimensions and age groups. Significant differences were found in the academic, emotional, family, and physical dimensions between educational stages and between the scale as a single construct. Significant inverse correlations were also found between age groups and dimensions. Overall self-concept decreases with age and varies according to the educational stage.
What’s New in the Classification, Diagnosis and Therapy of Myeloid Leukemias
Authors: Pizzi Marco , Gurrieri Carmela , Orazi Attilio
Published: March 2023

Myeloid leukemias are a broad group of hematological disorders, characterized by heterogeneous clinical and biological features. In recent years, unprecedented genetic discoveries and clinical–biological correlations have revolutionized the field of myeloid leukemias. The most relevant changes have specifically occurred in acute myeloid leukemia (AML), chronic myelomonocytic leukemia (CMML), chronic myeloid leukemia (CML) and myeloid neoplasms (MNs) with eosinophilia. The recently published International Consensus Classification (ICC) of myeloid neoplasms has addressed these changes, providing an updated framework and revised diagnostic criteria for such entities. This is also the aim of the 5th edition of the WHO classification of hematopoietic tumors, whose preliminary version was published in 2022. Parallel to this, new therapeutic options and novel molecular targets have changed the management of many myeloid entities, including AML and CML. This review aims to address the most relevant updates in the classification and diagnosis of AML, CMML, CML and MNs with eosinophilia. The state of the art of treatment and future therapeutic options for such disorders are also discussed.
Improvement of Nutritional Quality of Tomato Fruit with Funneliformis mosseae Inoculation under Greenhouse Conditions
Authors: Ullah Fazal , Ullah Habib , Ishfaq Muhammad , Gul Syeda Leeda , Kumar Tanweer , Li Zhifang
Published: March 2023

Long-term soil mining with extensive cultivation practices and traditional breeding methods have declined the flavor and nutritional value of tomatoes. Apart from important mineral nutrients (i.e., nitrogen, phosphorus, and potassium), fungi known as arbuscular mycorrhizae (AM) can considerably improve the quality of agricultural production through higher phosphate uptake. Using hydroponically cultured commercially available tomato cultivars, we investigated the possible effects of mycorrhizae in improving the nutritional quality of tomato fruit. Funneliformis mosseae (syn. Glomus mosseae)-inoculated tomato plants were grown on a 1:1 mixture of peat and vermiculite, and different phosphorus levels were applied. RNAseq and metabolites were studied to confirm the relative gene expression and metabolites in fruit tissues. The results showed that AM inoculation with low phosphorus can significantly improve important fruit-quality traits such as free amino acids, lycopene (47.9%), and β-carotene (29.6%) without compromising the yield. Further, differentially expressed genes (DEGs) were identified by comparing the nutritional and ripening potential of fruits produced by mycorrhizal and non-mycorrhizal plants. Notably, carotenoids and sugars (BRIX values) were found to be higher in mycorrhized plants in contrast to non-mycorrhized plants. Therefore, the current study suggests mycorrhization as a promising approach for the production of high-quality tomato fruit for human consumption.
A Systematic Review of Joint Spatial and Spatiotemporal Models in Health Research
Authors: Tesema Getayeneh Antehunegn , Tessema Zemenu Tadesse , Heritier Stephane , Stirling Rob G. , Earnest Arul
Published: March 2023

With the advancement of spatial analysis approaches, methodological research addressing the technical and statistical issues related to joint spatial and spatiotemporal models has increased. Despite the benefits of spatial modelling of several interrelated outcomes simultaneously, there has been no published systematic review on this topic, specifically when such models would be useful. This systematic review therefore aimed at reviewing health research published using joint spatial and spatiotemporal models. A systematic search of published studies that applied joint spatial and spatiotemporal models was performed using six electronic databases without geographic restriction. A search with the developed search terms yielded 4077 studies, from which 43 studies were included for the systematic review, including 15 studies focused on infectious diseases and 11 on cancer. Most of the studies (81.40%) were performed based on the Bayesian framework. Different joint spatial and spatiotemporal models were applied based on the nature of the data, population size, the incidence of outcomes, and assumptions. This review found that when the outcome is rare or the population is small, joint spatial and spatiotemporal models provide better performance by borrowing strength from related health outcomes which have a higher prevalence. A framework for the design, analysis, and reporting of such studies is also needed.
Quality-Adjusted Life Years in Erythropoietic Protoporphyria and Other Rare Diseases: A Patient-Initiated EQ-5D Feasibility Study
Authors: Barman-Aksözen Jasmin , Minder Anna-Elisabeth , Granata Francesca , Pettersson Mårten , Dechant Cornelia , Aksözen Mehmet Hakan , Falchetto Rocco
Published: March 2023

Erythropoietic protoporphyria (EPP) is an ultra-rare inborn error of metabolism characterised by painful phototoxic burn injuries after short exposure times to visible light. Patients with EPP are highly adapted to their condition which makes the quantification of their health-related quality of life (QoL) challenging. In the presented patient-initiated feasibility study, we describe a new approach to assess treatment benefits in EPP by measuring QoL with the generic EQ-5D instrument in five patients under long-term (≥two years) treatment with afamelanotide, the first approved therapy for EPP. For the study, we selected patients with EPP who in addition were affected by an involuntary treatment interruption (caused by a temporary reimbursement suspension) because we hypothesized that individuals who had previously unlearned their adaptation are better able to assess their life without treatment than treatment-naïve patients. QoL under treatment was comparable to the age-matched population norm, and retrospective results for a treatment interruption and phototoxic reaction time point were comparable to the QoL of patients with chronic neuropathic pain and acute burn injuries, respectively. The results were accepted by the National Institute for Health and Care Excellence in England for their evaluation of the cost-effectiveness of afamelanotide, i.e., the calculation of quality-adjusted life years.
Changes in Alcohol Consumption during the COVID-19 Pandemic: Evidence from Wisconsin
Authors: Pomazal Rachel , Malecki Kristen M. C. , McCulley Laura , Stafford Noah , Schowalter Mikayla , Schultz Amy
Published: March 2023

Alcohol consumption often increases in times of stress such as disease outbreaks. Wisconsin has historically ranked as one of the heaviest drinking states in the United States with a persistent drinking culture. Few studies have documented the impact of the COVID-19 pandemic on alcohol consumption after the first few months of the pandemic. The primary aim of this study is to identify factors related to changes in drinking at three timepoints during the first eighteen months of the pandemic. Survey data was collected from May to June 2020 (Wave 1), from January to February 2021 (Wave 2), and in June 2021 (Wave 3) among past participants of the Survey of the Health of Wisconsin. Study participants included 1290, 1868, and 1827 participants in each survey wave, respectively. Participants were asked how their alcohol consumption changed in each wave. Being younger, having anxiety, a bachelor’s degree or higher, having higher income, working remotely, and children in the home were significantly associated with increased drinking in all waves. Using logistic regression modeling, younger age was the most important predictor of increased alcohol consumption in each wave. Young adults in Wisconsin may be at higher risk for heavy drinking as these participants were more likely to increase alcohol use in all three surveys.
Managing Accident Prevention in Ski Resorts: Participants’ Actual Velocities in Slow Zones
Authors: Carus Luis , Mamaqi-Kapllani Xhevrije
Published: March 2023

Velocity is one of the main factors affecting the kinematic of snow sports’ accidents and the severity of resulting injuries. The aims of the present study were to measure the actual maximum velocities attained by a sample of snow sports participants in slow zones, to compare them to the recommended velocity limits and to assess whether their velocities were in any way related to their personal characteristics and to environmental conditions. Data were drawn from a sample of 1023 recreational skiers and snowboarders during the 2021–2022 winter season at four ski resorts located in the Spanish Pyrenees. Maximum velocity measurements were taken by the authors with a radar speed gun whose precision had been previously validated. Bivariate analysis tests were used to compare the influence that personal characteristics and environmental conditions had on the participants’ maximum velocities. Furthermore, a multivariate analysis was performed. The binary logistic regression was used to distinguish the categories of personal and environmental factors that have the highest probabilities of impact on different segments of velocity. As generally accepted, probability values were two-tailed, and values of 0.05 or less were regarded as statistically significant. Participants’ mean measured maximum velocity (±SD) was 51.61 (±16.14) km/h. A vast majority of the participants in this study traveled in slow zones at actual maximum velocities well over the recommended limits. Multivariate analysis showed that modality and both environmental conditions (visibility and snow quality) were highly significant and can be used to explain the chances of an increase in velocity in slow zones. Further research is needed to investigate causal relationships between skiers and snowboarders’ accidents, injuries and disrespect for velocity limits.
Dissemination and Implementation of a Text Messaging Campaign to Improve Health Disparities among Im/Migrant Workers
Authors: Cherryhomes Ellie , Guillot-Wright Shannon
Published: March 2023

The use of short message service (SMS) text messaging technology has grown in popularity over the last twenty years, but there is limited data on the design and feasibility of campaigns to reduce work-related injury, particularly among rural workers, non-native English speakers, and illiterate or low-literacy populations. Although there is a critical need for tech equity or ‘TechQuity’ interventions that reduce injury and enhance the wellbeing of under-reached communities, the barriers and benefits to implementation must be empirically and systematically examined. Thus, our team used D&I science to design and implement an 18-week texting campaign for under-reached workers with a higher-than-average risk of fatal and non-fatal injury. The experimental project was conducted with English-, Spanish-, and Vietnamese-speaking commercial fishermen in the Gulf of Mexico to test the design and feasibility, and messaging focused on preventing injury from slips, trips, and falls, as well as hurricane preparedness. The ubiquity of mobile devices and the previous success of texting campaigns made this a promising approach for enhancing health and preventing injury among an under-reached population. However, the perceived benefits were not without their barriers. The lessons learned included the difficulty of navigating federal regulations regarding limits for special characters, enrolling migratory participants, and navigating areas with limited cellular service or populations with limited accessibility to technology. We conclude with short- and long-term suggestions for future technology interventions for under-reached worker populations, including ethical and policy regulations.
Predictors of Mental Health Service Utilization among Frontline Healthcare Workers during the COVID-19 Pandemic
Authors: Starkweather Sydney , DePierro Jonathan M. , Akhtar Saadia , de Guillebon Eleanore , Kaplan Carly , Kaplan Sabrina , Ripp Jonathan , Peccoralo Lauren , Feingold Jordyn , Feder Adriana , Murrough James W. , Pietrzak Robert H.
Published: March 2023

(1) Background: This study examined the prevalence and correlates of factors associated with self-reported mental health service use in a longitudinal cohort of frontline health care workers (FHCWs) providing care to patients with COVID-19 throughout 2020. (2) Methods: The study comprised a two-wave survey (n = 780) administered in April–May 2020 (T1) and November 2020–January 2021 (T2) to faculty, staff, and trainees in a large urban medical center. Factors associated with initiation, cessation, or continuation of mental health care over time were examined. (3) Results: A total of 19.1% of FHCWs endorsed currently utilizing mental health services, with 11.4% continuing, 4.2% initiating, and 3.5% ceasing services between T1 and T2. Predisposing and need-related factors, most notably a history of a mental health diagnosis and distress related to systemic racism, predicted service initiation and continuation. Among FHCWs with a prior mental health history, those with greater perceived resilience were less likely to initiate treatment at T2. Descriptive data highlighted the importance of services around basic and safety needs (e.g., reliable access to personal protective equipment) relative to mental health support in the acute phase of the pandemic. (4) Conclusions: Results may be helpful in identifying FHCWs who may benefit from mental health services.
A Spatio-Temporal Dynamic Visualization Method of Time-Varying Wind Fields Based on Particle System
Authors: Chu Lele , Ai Bo , Wen Yubo , Shi Qingtong , Ma Huadong , Feng Wenjun
Published: March 2023

The particle system is widely used in vector field feature visualization due to its dynamics and simulation. However, there are some defects of the vector field visualization method based on the Euler fields, such as unclear feature expression and discontinuous temporal expression, so the method cannot effectively express the characteristics of wind field on the temporal scale. We propose a Lagrangian visualization method based on spatio-temporal interpolation to solve these problems, which realizes the fusion and expression of the particle system and the time-varying wind data based on the WebGL shader. Firstly, the linear interpolation algorithm is used to interpolate to obtain continuous and dense wind field data according to the wind field data at adjacent moments. Then, we introduce the Lagrangian analysis method to study the structure of the wind field and optimize the visualization effect of the particle system based on Runge–Kutta algorithms. Finally, we adopt the nonlinear color mapping method with double standard deviation (2SD) to improve the expression effect of wind field features. The experimental results indicate that the wind visualization achieves a comprehensive visual effect and the rendering frame rate is greater than 45. The methods can render the particles smoothly with stable and outstanding uniformity when expressing continuous spatio-temporal dynamic visualization characteristics of the wind field.
Interaction of Terminal Oxidases with Amphipathic Molecules
Authors: Azarkina Natalia V. , Borisov Vitaliy B. , Oleynikov Ilya P. , Sudakov Roman V. , Vygodina Tatiana V.
Published: March 2023

The review focuses on recent advances regarding the effects of natural and artificial amphipathic compounds on terminal oxidases. Terminal oxidases are fascinating biomolecular devices which couple the oxidation of respiratory substrates with generation of a proton motive force used by the cell for ATP production and other needs. The role of endogenous lipids in the enzyme structure and function is highlighted. The main regularities of the interaction between the most popular detergents and terminal oxidases of various types are described. A hypothesis about the physiological regulation of mitochondrial-type enzymes by lipid-soluble ligands is considered.
Nd-Doped ZnO Nanostructures with Enhanced Photocatalytic Performance for Environmental Protection
Authors: Pascariu Petronela , Cojocaru Corneliu , Samoila Petrisor , Romanitan Cosmin
Published: March 2023

Neodymium (Nd)-doped ZnO nanostructures with different amounts of Nd were obtained by the electrospinning–calcination method. X-ray diffraction measurements indicated that the prepared nanostructures have a wurtzite structure without undesirable impurities. Nd doping changes the mean crystallite size as well the lattice strain, as proved by Williamson–Hall plots. The ZnO-based nanostructures were tested as photocatalysts for methylene blue (MB) dye and ciprofloxacin (CIP) drug pollutant degradations under visible light irradiation. Corroborating the obtained results, it was found that the reaction rate constant increased almost linearly with the mean crystallite size (from 2.235 × 10−2 to 3.482 × 10−2 min−1) with a variation in the mean crystallite size from 24.2 to 42.1 nm. Furthermore, the best catalyst sample (0.1% Nd-doped ZnO) was used to optimize the photodegradation process of ciprofloxacin, taking into account the pollutant concentration as well as the catalyst dose. The removal efficiency after 120 min was about 100%, with the rate constant of k = 5.291·10−2 min−1 (CIP) and k = 4.780·10−2 min−1 (MB) for the established optimal conditions. Considering the value of the rate constant, the half-life of the reaction (τ1/2 = ln2/k) was evaluated to be about τ1/2 =13 min for CIP and 14.5 min corresponding to MB. Several catalytic cycles were successfully performed without any loss of photocatalytic activity using these nanostructures, demonstrating that the obtained nanostructures have good stability in the leaching processes.
A Leucine-Rich Repeat Receptor-like Kinase TaBIR1 Contributes to Wheat Resistance against Puccinia striiformis f. sp. tritici
Authors: Sun Yingchao , Wang Xiaojie , Liu Feiyang , Guo Haoyu , Wang Jianfeng , Wei Zetong , Kang Zhensheng , Tang Chunlei
Published: March 2023

Plant cell surface-localized receptor-like kinases (RLKs) recognize invading pathogens and transduce the immune signals inside host cells, subsequently triggering immune responses to fight off pathogen invasion. Nonetheless, our understanding of the role of RLKs in wheat resistance to the biotrophic fungus Puccinia striiformis f. sp. tritici (Pst) remains limited. During the differentially expressed genes in Pst infected wheat leaves, a Leucine-repeat receptor-like kinase (LRR-RLK) gene TaBIR1 was significantly upregulated in the incompatible wheat-Pst interaction. qRT-PCR verified that TaBIR1 is induced at the early infection stage of Pst. The transient expression of TaBIR1-GFP protein in N. bentamiana cells and wheat mesophyll protoplasts revealed its plasma membrane location. The knockdown of TaBIR1 expression by VIGS (virus induced gene silencing) declined wheat resistance to stripe rust, resulting in reduced reactive oxygen species (ROS) production, callose deposition, and transcripts of pathogenesis-related genes TaPR1 and TaPR2, along with increased Pst infection area. Ectopic overexpression of TaBIR1 in N. benthamiana triggered constitutive immune responses with significant cell death, callose accumulation, and ROS production. Moreover, TaBIR1 triggered immunity is dependent on NbBAK1, the silencing of which significantly attenuated the defense response triggered by TaBIR1. TaBIR1 interacted with the NbBAK1 homologues in wheat, co-receptor TaSERK2 and TaSERK5, the transient expression of which could restore the impaired defense due to NbBAK1 silencing. Taken together, TaBIR1 is a cell surface RLK that contributes to wheat stripe rust resistance, probably as a positive regulator of plant immunity in a BAK1-dependent manner.
Complexity of the Immune Response Elicited by Different COVID-19 Vaccines, in the Light of Natural Autoantibodies and Immunomodulatory Therapies
Authors: Böröcz Katalin , Kinyó Ágnes , Simon Diana , Erdő-Bonyár Szabina , Németh Péter , Berki Timea
Published: March 2023

Despite the abundance of data on the COVID-19 vaccine-induced immune activation, the impact of natural autoantibodies (nAAbs) on these processes is less well defined. Therefore, we investigated potential connections between vaccine efficacy and nAAb levels. We were also interested in the impact of immunomodulatory therapies on vaccine efficacy. Clinical residual samples were used for the assessment of the COVID-19 vaccine-elicited immune response (IR) (n=255), as well as for the investigation of the immunization-associated expansion of the nAAb pool (n=185). In order to study the potential interaction between immunomodulatory therapies and the vaccine-induced IR, untreated, healthy individuals and patients receiving anti-TNFα or anti-IL-17 therapies were compared (n total =45). In-house ELISAs (anticitrate synthase, anti-HSP60 and-70) and commercial ELISAs (anti-SARS-CoV-2 ELISAs IgG, IgA, NeutraLISA and IFN-γ release assay ‘IGRA’) were applied. We found significant differences in the IR given to different vaccines. Moreover, nAAb levels showed plasticity in response to anti-COVID-19 immunization. We conclude that our findings may support the theorem about the non-specific beneficial ‘side effects’ of vaccination, including the broadening of the nAAb repertoire. Considering immunomodulation, we suggest that anti-TNFα and anti-IL17 treatments may interfere negatively with MALT-associated IR, manifested as decreased IgA titers; however, the modest sample numbers of the herein presented model might be a limiting factor of reaching a more comprehensive conclusion.
Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs
Authors: Sulaiman Raed , De Pradip , Aske Jennifer C. , Lin Xiaoqian , Dale Adam , Gaster Kris , Espaillat Luis Rojas , Starks David , Dey Nandini
Published: March 2023

Cancer-associated fibroblasts (CAFs) within a solid tumor can support the progression of cancer. We studied the identification and characterization of patient-derived endometrial CAFs in the context of their clinical relevance in endometrial cancers. We established patient-derived primary cultures of CAFs from surgically resected tumors (TCAF) and tumor-adjacent normal (NCAF) tissues in 53 consented patients with success rates of 97.7% and 75%, respectively. A passage of CAF was qualified by the (1) absence of CK 8,18,19, EpCAM, CD45, and CD31, and (2) presence of SMAalpha, S100A4, CD90, FAP, TE-7, CD155, PD-L1, TGFB, PDGFRA (qRT-PCR, flow cytometry, Western blot, ICC). Out of the 44 established CAFs, 31 were aggressive (having an early, i.e., 4–7 week, establishment time and/or >3 passages) compared to 13 which were non-aggressive. A post-surgery-event (PSE) was observed in 7 out of 31 patients bearing aggressive CAFs, 2 of whom were also positive for CTCs, while none of the 13 patients bearing non-aggressive CAFs had events. A positive correlation was found between patients with grade 3 (p = 0.025) as well as stage 3/4 diseases (p = 0.0106) bearing aggressive CAFs and the PSE. Finally, aggressive TCAFs from patients with PSE resisted the effects of paclitaxel and lenvatinib on the growth of HUVEC and endometrial tumor cells. Our study is the first to report a correlation between the PSE and the aggressive nature of CAFs in endometrial cancers and provides an undeniable reason to study the in-depth mechanism of CAF function towards the development of treatment resistance in endometrial cancers.
Atrial Natriuretic Peptides as a Bridge between Atrial Fibrillation, Heart Failure, and Amyloidosis of the Atria
Authors: Rahbar Kouibaran Farzad , Sabatino Mario , Barozzi Chiara , Diemberger Igor
Published: March 2023

ANP is mainly synthesized by the atria, and upon excretion, it serves two primary purposes: vasodilation and increasing the renal excretion of sodium and water. The understanding of ANP’s role in cardiac systems has improved considerably in recent decades. This review focuses on several studies demonstrating the importance of analyzing the regulations between the endocrine and mechanical function of the heart and emphasizes the effect of ANP, as the primary hormone of the atria, on atrial fibrillation (AF) and related diseases. The review first discusses the available data on the diagnostic and therapeutic applications of ANP and then explains effect of ANP on heart failure (HF) and atrial fibrillation (AF) and vice versa, where tracking ANP levels could lead to understanding the pathophysiological mechanisms operating in these diseases. Second, it focuses on conventional treatments for AF, such as cardioversion and catheter ablation, and their effects on cardiac endocrine and mechanical function. Finally, it provides a point of view about the delayed recovery of cardiac mechanical and endocrine function after cardioversion, which can contribute to the occurrence of acute heart failure, and the potential impact of restoration of the sinus rhythm by extensive ablation or surgery in losing ANP-producing sites. Overall, ANP plays a key role in heart failure through its effects on vasodilation and natriuresis, leading to a decrease in the activity of the renin-angiotensin-aldosterone system, but it is crucial to understand the intimate role of ANP in HF and AF to improve their diagnosis and personalizing the patients’ treatment.
Zirconia-Toughened Alumina Ceramic Wear Particles Do Not Elicit Inflammatory Responses in Human Macrophages
Authors: Porporati Alessandro Alan , Mödinger Yvonne , Fischer Sarah , Polajžer Sara , Mettang Melanie , Deisinger Ulrike , Podlogar Matejka , Trebše Rihard , Lovšin Nika
Published: March 2023

Ten percent of patients undergoing total hip arthroplasty (THA) require revision surgery. One of the reasons for THA are wear particles released from the implants that can activate the immune defense and cause osteolysis and failure of the joint implant. The discrepancies between reports on toxicity and immunogenicity of the implant materials led us to this study in which we compared toxicity and immunogenicity of well-defined nanoparticles from Al2O3, zirconia-toughened alumina (ZTA), and cobalt chrome (CoCr), a human THP-1 macrophage cell line, human PBMCs, and therefrom-derived primary macrophages. None of the tested materials decreased the viability of THP-1 macrophages nor human primary macrophages at the 24 h time point, indicating that at concentrations from 0.05 to 50 µm3/cell the tested materials are non-toxic. Forty-eight hours of treatment of THP-1 macrophages with 5 µm3/cell of CoCr and Al2O3 caused 8.3-fold and 4.6-fold increases in TNF-α excretion, respectively, which was not observed for ZTA. The comparison between THP-1 macrophages and human primary macrophages revealed that THP-1 macrophages show higher activation of cytokine expression in the presence of CoCr and Al2O3 particles than primary macrophages. Our results indicate that ZTA is a non-toxic implant material with no immunogenic effects in vitro.
Ion Channels as a Potential Target in Pharmaceutical Designs
Authors: Wu Sheng-Nan
Published: March 2023

Voltage-gated ion channels are integral membrane proteins that respond to changes in membrane potential with rapid variations in membrane permeability to ions [...]
Thalidomide Attenuates Epileptogenesis and Seizures by Decreasing Brain Inflammation in Lithium Pilocarpine Rat Model
Authors: Cumbres-Vargas Irán M. , Zamudio Sergio R. , Pichardo-Macías Luz A. , Ramírez-San Juan Eduardo
Published: March 2023

Thalidomide (TAL) has shown potential therapeutic effects in neurological diseases like epilepsy. Both clinical and preclinical studies show that TAL may act as an antiepileptic drug and as a possible treatment against disease development. However, the evidence for these effects is limited. Therefore, the antiepileptogenic and anti-inflammatory effects of TAL were evaluated herein. Sprague Dawley male rats were randomly allocated to one of five groups (n = 18 per group): control (C); status epilepticus (SE); SE-TAL (25 mg/kg); SE-TAL (50 mg/kg); and SE-topiramate (TOP; 60mg/kg). The lithium-pilocarpine model was used, and one day after SE induction the rats received pharmacological treatment for one week. The brain was obtained, and the hippocampus was micro-dissected 8, 18, and 28 days after SE. TNF-α, IL-6, and IL-1β concentrations were quantified. TOP and TAL (50 mg/kg) increased the latency to the first of many spontaneous recurrent seizures (SRS) and decreased SRS frequency, as well as decreasing TNF-α and IL-1β concentrations in the hippocampus. In conclusion, the results showed that both TAL (50 mg/kg) and TOP have anti-ictogenic and antiepileptogenic effects, possibly by decreasing neuroinflammation.
The Cracking Behavior of Two Dental Composite Materials Validated through Multifractal Analyzes
Authors: Nica Irina , Nedeff Florin , Nedeff Valentin , Popa Cristina , Toma Ștefan Lucian , Agop Maricel , Vasincu Decebal
Published: March 2023

The aim of this in vitro study was to analyze, both experimentally and theoretically, the mechanical behavior of two types of composite materials used in restoring dental integrity. The samples of each composite resin, namely Filtek Supreme XT (3M ESPE, St. Paul, MN, USA) and Filtek Z250 (3M ESPE, St. Paul, MN, USA), were experimentally analyzed by determining their compressive strength and fracture behavior. The fractured fragments of the samples were subjected to surface evaluation by scanning electron microscopy. The compressive stress—compressive strain dependencies revealed stronger cracking of the Filtek Supreme XT composite than Filtek Z250 prior to fracture. Theoretically, the evaluation was made by means of holographic implementations of such types of composite materials. A Hooke-type equation in a differential form is presented, which links the proposed theoretical model with the experimentally obtained data.
Impact of Environmental and Epigenetic Changes on Mesenchymal Stem Cells during Aging
Authors: Smith Nicholas , Shirazi Suzanna , Cakouros Dimitrios , Gronthos Stan
Published: March 2023

Many crucial epigenetic changes occur during early skeletal development and throughout life due to aging, disease and are heavily influenced by an individual’s lifestyle. Epigenetics is the study of heritable changes in gene expression as the result of changes in the environment without any mutation in the underlying DNA sequence. The epigenetic profiles of cells are dynamic and mediated by different mechanisms, including histone modifications, non-coding RNA-associated gene silencing and DNA methylation. Given the underlining role of dysfunctional mesenchymal tissues in common age-related skeletal diseases such as osteoporosis and osteoarthritis, investigations into skeletal stem cells or mesenchymal stem cells (MSC) and their functional deregulation during aging has been of great interest and how this is mediated by an evolving epigenetic landscape. The present review describes the recent findings in epigenetic changes of MSCs that effect growth and cell fate determination in the context of aging, diet, exercise and bone-related diseases.
Adaptive Kernel Graph Nonnegative Matrix Factorization
Authors: Li Rui-Yu , Guo Yu , Zhang Bin
Published: March 2023

Nonnegative matrix factorization (NMF) is an efficient method for feature learning in the field of machine learning and data mining. To investigate the nonlinear characteristics of datasets, kernel-method-based NMF (KNMF) and its graph-regularized extensions have received much attention from various researchers due to their promising performance. However, the graph similarity matrix of the existing methods is often predefined in the original space of data and kept unchanged during the matrix-factorization procedure, which leads to non-optimal graphs. To address these problems, we propose a kernel-graph-learning-based, nonlinear, nonnegative matrix-factorization method in this paper, termed adaptive kernel graph nonnegative matrix factorization (AKGNMF). In order to automatically capture the manifold structure of the data on the nonlinear feature space, AKGNMF learned an adaptive similarity graph. We formulated a unified objective function, in which global similarity graph learning is optimized jointly with the matrix decomposition process. A local graph Laplacian is further imposed on the learned feature subspace representation. The proposed method relies on both the factorization that respects geometric structure and the mapped high-dimensional subspace feature representations. In addition, an efficient iterative solution was derived to update all variables in the resultant objective problem in turn. Experiments on the synthetic dataset visually demonstrate the ability of AKGNMF to separate the nonlinear dataset with high clustering accuracy. Experiments on real-world datasets verified the effectiveness of AKGNMF in three aspects, including clustering performance, parameter sensitivity and convergence. Comprehensive experimental findings indicate that, compared with various classic methods and the state-of-the-art methods, the proposed AKGNMF algorithm demonstrated effectiveness and superiority.
Spider Ecology and Behaviour—Spiders as Model Organisms
Authors: Hesselberg Thomas , Gálvez Dumas
Published: March 2023

Spiders are versatile and ubiquitous generalist predators that can be found in all terrestrial ecosystems except for Antarctica [...]
Imaging Approaches and the Quantitative Analysis of Heart Development
Authors: Raiola Morena , Sendra Miquel , Torres Miguel
Published: March 2023

Heart morphogenesis is a complex and dynamic process that has captivated researchers for almost a century. This process involves three main stages, during which the heart undergoes growth and folding on itself to form its common chambered shape. However, imaging heart development presents significant challenges due to the rapid and dynamic changes in heart morphology. Researchers have used different model organisms and developed various imaging techniques to obtain high-resolution images of heart development. Advanced imaging techniques have allowed the integration of multiscale live imaging approaches with genetic labeling, enabling the quantitative analysis of cardiac morphogenesis. Here, we discuss the various imaging techniques used to obtain high-resolution images of whole-heart development. We also review the mathematical approaches used to quantify cardiac morphogenesis from 3D and 3D+time images and to model its dynamics at the tissue and cellular levels.
Effects of Probiotics on Intermediate Cardiovascular Outcomes in Patients with Overweight or Obesity: A Systematic Review and Meta-Analysis
Authors: Mayta-Tovalino Frank , Diaz-Arocutipa Carlos , Piscoya Alejandro , Hernandez Adrian V.
Published: March 2023

Background: Clinical trials evaluating the effect of probiotics on cardiovascular intermediate outcomes have been scarce in recent years. We systematically evaluated the efficacy of probiotics on intermediate cardiovascular outcomes in patients with overweight or obesity. Methods: We searched for randomized controlled trials (RCTs) in four databases (until August 2021) that evaluated the effects of probiotics versus controls on intermediate cardiovascular outcomes. The outcomes were body mass index (BMI), weight, systolic blood pressure (SBP), diastolic blood pressure (DBP), glucose, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels. Inverse variance random effects meta-analyses were used. The effects were reported as mean difference (MD), with their 95% confidence intervals (95% CI). The quality of evidence (QoE) was assessed with GRADE (grading of recommendations, assessment, development and evaluations) methodology. Results: A total of 25 RCTs were included (n = 2170), with a range of follow-up from two to six months. Probiotics likely reduced BMI (MD −0.27 kg/m2, 95%CI: −0.35 to −0.19; 17 RCTs; I2 = 26%, QoE: moderate), as well as likely reduced weight (MD −0.61 kg, 95%CI: −0.89 to −0.34; 15 RCTs; I2 = 0%, QoE: moderate), and may have slightly reduce LDL (MD −4.08 mg/dL; 95%CI: −6.99 to −1.17; 9 RCTs; I2 = 87%, QoE: low) in comparison to the controls. However, probiotics had no effect on SBP (MD −0.40 mmHg; 95%CI: −5.04 to 4.25; 7 RCTs; I2 = 100%, QoE: very low), DBP (MD −1.73 mmHg; 95%CI: −5.29 to 1.82; 5 RCTs; I2 = 98%, QoE: very low), glucose (MD −0.07 mg/dL; 95%CI −0.89 to 0.75; I2 = 96%, QoE: very low), HDL (MD −1.83 mg/dL; 95%CI: −4.14 to 2.47; 14 RCTs; I2 = 98%, QoE: very low), or triglycerides (MD −3.29 mg/dL, 95%CI −17.03 to 10.45; 14 RCTs, I2 = 95%, QoE: very low) compared to control arms, and the evidence was very uncertain. Conclusions: In obese or overweight patients, BMI, weight, and LDL were lower in patients who received probiotics compared to those who received controls. Other lipids, glucose, and blood pressure were not affected by the probiotics.
The Prevention, Diagnosis, and Treatment of Rapid Oral Health Deterioration (ROHD) among Older Adults
Authors: Marchini Leonardo , Ettinger Ronald L.
Published: March 2023

The world’s population is aging. Older adults are at risk for multiple chronic medical problems as they age. The management of these diseases requires these people to take a variety of medications, which may have undesired side effects. These medical issues can impact oral healthcare and result in a precipitous decline in oral health. A standardized teaching model has been developed to help novice dental practitioners learn how to access and treat oral health problems in older adults. This model is called rapid oral health deterioration (ROHD) risk assessment. The model has four steps for assessment and four categories of risk. This paper describes the components of the ROHD risk assessment, and how it can be used to prevent, diagnose and treat ROHD among older adults.
Challenges of the Effectiveness of Traumatic Brain Injuries Biomarkers in the Sports-Related Context
Authors: Tomaiuolo Rossella , Zibetti Martina , Di Resta Chiara , Banfi Giuseppe
Published: March 2023

Traumatic brain injury affects 69 million people every year. One of the main limitations in managing TBI patients is the lack of univocal diagnostic criteria, including the absence of standardized assessment methods and guidelines. Computerized axial tomography is the first-choice examination, despite the limited prevalence of positivity; moreover, its performance is undesirable due to the risk of radiological exposure, prolonged stay in emergency departments, inefficient use of resources, high cost, and complexity. Furthermore, immediacy and accuracy in diagnosis and management of TBIs are critically unmet medical needs. Especially in the context of sports-associated TBI, there is a strong need for prognostic indicators to help diagnose and identify at-risk subjects to avoid their returning to play while the brain is still highly vulnerable. Fluid biomarkers may emerge as new prognostic indicators to develop more accurate prediction models, improving risk stratification and clinical decision making. This review describes the current understanding of the cellular sources, temporal profile, and potential utility of leading and emerging blood-based protein biomarkers of TBI; its focus is on biomarkers that could improve the management of mild TBI cases and can be measured readily and directly in the field, as in the case of sports-related contexts.
Sleep Bruxism in Children—What Can Be Learned from Anamnestic Information
Authors: Emodi-Perlman Alona , Shreiber-Fridman Yarden , Kaminsky-Kurtz Shani , Eli Ilana , Blumer Sigalit
Published: March 2023

Sleep bruxism (SB) is a masticatory muscle activity during sleep, and its clinical manifestation in young children is still unclear. The aim of the present study was to evaluate the role of anamnestic information in predicting possible SB in children aged 4–12 years. In a cross-sectional retrospective exploratory study, the dental files of 521 children were examined with regard to the following anamnestic information: gender, age, medical conditions associated with ear, nose, and throat (ENT), respiratory disorders, use of methylphenidate (Ritalin), oral habits, and bruxing during sleep. A child was defined as presenting possible SB when a positive report was received from parents regarding such behavior (SB positive, No. = 84). There were no age- and/or gender-wise differences between SB-positive children and children whose parents did not report SB behavior (SB negative). SB-positive children suffered more from ENT and respiratory disorders than children without SB. Additionally, the use of pacifiers/finger sucking, as well as snoring, were more common among SB-positive children as compared to their SB-negative counterparts (Chi-square). The variables which were found to significantly increase the odds of possible SB in children were mouth breathing, ENT problems, and use of a pacifier or finger sucking (forward stepwise logistic regression). Clinicians should look for clinical signs of possible SB in children whose anamnesis reveals one or more of these anamnestic signals.
Alcohol Consumption within 48 hours before Onset Is Associated with Adverse Clinical Outcomes in Hypertriglyceridemic Pancreatitis
Authors: Lai Tianming , Zhu Yin , Lu Nonghua , He Wenhua
Published: March 2023

(1) Background: Some patients with hypertriglyceridemic pancreatitis (HTGP) drink occasionally or moderately, but do not meet the diagnostic criteria for alcoholic pancreatitis. This study aims to investigate whether occasional or moderate alcohol consumption affects the clinical outcomes of patients with HTGP. (2) Methods: This retrospective study included 373 patients with HTGP from January 2007 to December 2021. HTGP patients with occasional or moderate alcohol (OMA) consumption before onset were divided into the OMA group, and HTGP patients without alcohol (WA) consumption were divided into the WA group. The OMA group was further divided into two groups: the drinking within 48 h before onset (DW) group, and the without drinking within 48 h before onset (WDW) group. The clinical data of the two groups were compared and multivariable logistic regression was used to analyze independent risk factors for the primary outcomes. (3) Results: The proportion of men (95.7% vs. 67.6%, p < 0.001) and smoking history (61.7% vs. 15.1%, p < 0.001) in the OMA group were higher than those in the WA group. Occasional or moderate alcohol consumption was independently associated with a high incidence of SAP (adjusted odds ratio (AdjOR), 1.57; 95% CI, 1.02–2.41; p = 0.041), and necrotizing pancreatitis (AdjOR, 1.60; 95% CI, 1.04–2.48; p = 0.034). After dividing the OMA group into two subgroups, we found that drinking within 48 h before onset was independently associated with a high incidence of SAP (AdjOR, 3.09; 95% CI, 1.66–5.77; p < 0.001), and necrotizing pancreatitis (AdjOR, 2.71; 95% CI, 1.46–5.05; p = 0.002). (4) Conclusion: Occasional or moderate alcohol consumption is associated with poor clinical outcomes in patients with HTGP, particularly if they drank alcohol within 48 h before the onset of the disease.
A Comparative Study of Temporomandibular Joints in Adults with Definite Sleep Bruxism on Magnetic Resonance Imaging and Cone-Beam Computer Tomography Images
Authors: Zhang Juan , Yu Wenjuan , Wang Jianghong , Wang Sijia , Li Yifan , Jing Huimin , Li Zekui , Li Xin , Liang Meng , Wang Yonglan
Published: March 2023

Objective: The aim was to study the imaging characteristics of the temporomandibular joint (TMJ) of definite sleep bruxers through magnetic resonance imaging (MRI) and cone-beam computer tomography (CBCT). Methods: Nineteen definite sleep bruxers diagnosed by polysomnography and twenty asymptomatic non-bruxers matched by age, gender, and education level participated in this study. After obtaining MRI and CBCT images of all TMJs of the subjects, evaluation and measurement were conducted, respectively. The analyzed parameters included disc position, disc configuration, joint effusion (JE), joint space or condyle position, and condylar bony changes. Results: Of the 38 joints in the study group, disc deformity and disc displacement of TMJs were both 57.9% when the mouth was closed, and 76.3% showed condylar bony changes, while when the mouth was open, 82% of all TMJs showed physiological biconcave discs. Comparison of joint space revealed that the anterior space was larger in the study group. There was no significant difference between the mild and the moderate to severe sleep bruxism subgroups in the changes of TMJ. Conclusion: The results demonstrated that a higher prevalence of disc deformity, disc displacement, JE, and condylar bony changes occurred in temporomandibular joints of sleep bruxers. These changes were not related to the severity of sleep bruxism.
Arrhythmic Manifestations of Cardiac Amyloidosis: Challenges in Risk Stratification and Clinical Management
Authors: Laptseva Natallia , Rossi Valentina A. , Sudano Isabella , Schwotzer Rahel , Ruschitzka Frank , Flammer Andreas J. , Duru Firat
Published: March 2023

Amyloidosis is a systemic disease characterized by extracellular deposits of insoluble amyloid in various tissues and organs. Cardiac amyloidosis is a frequent feature of the disease, causing a progressive, restrictive type of cardiomyopathy, and is associated with adverse clinical outcomes and increased mortality. The typical clinical presentation in patients with cardiac amyloidosis is heart failure (HF) with preserved ejection fraction. Most patients present with typical symptoms and signs of HF, such as exertional dyspnea, pretibial edema, pleural effusions and angina pectoris due to microcirculatory dysfunction. However, patients may also frequently encounter various arrhythmias, such as atrioventricular nodal block, atrial fibrillation and ventricular tachyarrhythmias. The management of arrhythmias in cardiac amyloidosis patients with drugs and devices is often a clinical challenge. Moreover, predictors of life-threatening arrhythmic events are not well defined. This review intends to give a deepened insight into the arrhythmic features of cardiac amyloidosis by discussing the pathogenesis of these arrhythmias, addressing the challenges in risk stratification and strategies for management in these patients.