bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunotherapy for sarcomas
Authors: Nakata Eiji , Fujiwara Tomohiro , Kunisada Toshiyuki , Ito Tastuo , Takihira Shota , Ozaki Toshifumi
Published: February 2021

Sarcomas are a heterogeneous group of malignancies of mesenchymal origin; their molecular and genomic mechanisms differ with regard to histology. These characteristics lead to the presentation of varied immunological profiles based on the tumor microenvironment. Various immunotherapies are considered for the treatment of sarcoma. These treatments are performed either in isolation or in combination with other methods such as cytotoxic chemotherapy or the use of molecular target agents. Among these, two recently emerging immunotherapies include T-cell receptor gene therapy and immune checkpoint inhibitor therapy, which are expected to be effective for many types of sarcoma. A sarcoma with a disease-specific translocation and a limited number of mutations, such as synovial sarcoma, expresses high levels of self-antigens, like the New York esophageal squamous cell carcinoma 1, which has been targeted in T-cell receptor gene therapy. On the other hand, sarcomas with a greater number of mutations, such as undifferentiated pleomorphic sarcomas, myxofibrosarcoma and dedifferentiated liposarcomas, can be good candidates for immune checkpoint inhibitors. Among immune checkpoint inhibitor therapies, programmed cell death-1 blockade (nivolumab and pembrolizumab) and cytotoxic T-lymphocyte–associated antigen 4 blockade (ipilimumab) have been investigated most often in sarcoma. Although the sole use of immune checkpoint inhibitors provides limited efficacy, combined immunotherapy with immune checkpoint inhibitors or molecular target agents, especially antiangiogenic agents, has shown moderate results against some types of sarcoma, such as the alveolar soft part sarcoma. Several clinical trials utilizing immunotherapy, including T-cell receptor gene therapy and immune checkpoint inhibitors, in sarcomas are under progress. By clarifying the tumor microenvironment and biomarker-predictive capacity of immunotherapy in sarcomas, better clinical trials can be designed; this could lead to improved outcomes for immunotherapy in sarcoma.
Adverse Events and Perception of Benefit From Duloxetine for Treating Aromatase Inhibitor-Associated Arthralgias
Authors: Schnell Patrick M , Lustberg Maryam B , Henry N Lynn
Published: February 2021

Background

Duloxetine effectively treats aromatase inhibitor-associated musculoskeletal symptoms (AIMSS) in women with breast cancer but causes low-grade toxicities. This secondary analysis examines the relationship between adverse events (AE) and patient-perceived benefit, based on patient self-report that the treatment received was beneficial despite side effects. We hypothesized that duloxetine had a favorable effect on patient-perceived benefit, even among duloxetine-treated patients who experienced AEs and who, had they been treated with placebo, would have experienced none.

Methods

Principal stratification was used to estimate the effect of duloxetine vs placebo on patient-perceived benefit and Functional Assessment of Cancer Therapy-Endocrine Scale functional quality of life in the randomized, double-blind trial SWOG S1202 (n = 289). Subgroups of patients were defined by observed and counterfactual (what would have occurred had they been randomly assigned to the opposite study arm) experiences of AEs and the original primary outcome, reduction of average pain after 12 weeks of at least 2 points on the Brief Pain Inventory-Short Form.

Results

Duloxetine caused an estimated 23.4% (95% credible interval [CI] = 13.4% to 33.7%) of patients to experience an AE even though they would have experienced none on placebo. Those patients remained more likely to report that their received treatment was beneficial than comparable patients assigned placebo (73.3% vs 41.8%, respectively; 95% CI for difference = 15.4 to 47.2 percentage points), although there was no statistically significant effect of duloxetine on functional quality of life (11.3 vs 9.0, 95% CI for difference = -2.2 to +6.7).

Conclusion

Duloxetine resulted in higher patient-perceived benefit, even among those who would have an AE on duloxetine but none on placebo. Treatment of AIMSS with duloxetine should be considered for appropriate patients.
Theoretical study on dynamic responses of an unlined circular tunnel subjected to blasting P-waves
Authors: Lu Shiwei , Zhou Chuanbo , Zhang Zhen , Ji Ling , Jiang Nan
Published: February 2021

In conventional studies, the blasting seismic wave is always treated as a time-harmonic wave, which is not suitable in some situations due to its short duration. In this paper, the blasting P-wave is simplified as a triangular impulse. The distribution functions of dynamic stress concentration factor (DSCF) and the radial and hoop vibration velocity scaling factors (RVSF and HVSF) around the circular tunnel are derived based on the Fourier–Bessel expansion method. Then, the effects of the rising duration, the total duration and Poisson's ratio on DSCF, RVSF and HVSF are discussed. Results show that (1) the maximum RVSF and HVSF are located at the incident side, and the location of the maximum DSCF will move from the incident side to the shadow side when the total duration increases; (2) the maximum DSCF increases with the increasing total duration, decreases with the increasing Poisson's ratio, but is immune to the ratio of the rising to total duration; (3) the maximum RVSF decreases with the increase of the total duration and the ratio of the rising to total duration, but increases with the increasing Poisson's ratio; (4) the maximum HVSF decreases with the increase of the total duration and the ratio of the rising to total duration, but is immune to Poisson's ratio; (5) the RVSF and HVSF under triangular impulses gradually change from less than those under time-harmonic waves to greater than those under time-harmonic waves with the increasing total duration, but the DSCF shows the opposite trend.
Effect of total glucosides of paeony and Tripterygium wilfordii polyglycosides on erythrocyte methotrexate polyglutamates in rats, analysed using ultra-high-performance liquid chromatography-tandem mass spectrometry
Authors: Wu Shulong , Mo Liqian , Ye Chunxiao , Xun Tianrong , Wang Xiaokang , Lv Bin , Zhan Xia , Liu Bin , Ding Qing , Peng Jun , Chen Congyan , Yang Xixiao
Published: February 2021

Objectives

The aim of the study was to explore the effect of total glucosides of paeony (TGP) and Tripterygium wilfordii polyglycosides (TWP) on erythrocyte methotrexate polyglutamates (MTXPGs), the metabolites of methotrexate (MTX).

Methods

An ultra-high-performance liquid chromatography (UPLC)-tandem mass spectrometry (MS/MS) method was developed to determine MTXPGs. The effects of MTXPGs were analysed using 24 male Sprague-Dawley rats that were randomly divided into the MTX alone, MTX-TGP combined, and MTX-TWP combined groups. Rats were administered MTX at a dose of 0.9 mg/kg once a week, TGP at 0.054 g/kg and TWP at 1.8 mg/kg three times a day. Venous blood (1.0 ml) was collected at weeks 2, 4, 6, 9, 12 and 15 and then analysed using the developed UPLC-MS/MS method.

Key findings

Specificity, linear range, inter-and intra-day precision, recovery, matrix effect and stability of MTXPGs met the standard regulations. This method was successfully used for the detection of MTXPGs. After administration of MTX alone, erythrocyte MTXPGs increased and accumulated in a time- and dose-dependent manner. Compared to MTX alone, the combination with TGP significantly decreased the content of total MTXPGs and short-chain MTXPGs (Methotrexate [MTX/MTXPG1] and 4-amino-10-methylpteroyldiglutamic acid [MTXPG2], P < 0.05), but had no significant effect on long-chain MTXPGs (4-amino-10-methylpteroyltriglutamic acid [MTXPG3], P > 0.05) and very long-chain MTXPGs (4-amino-10-methylpteroyltetraglutamic acid [MTXPG4] and 4-amino-10-methylpteroylpentaglutamic acid [MTXPG5], P > 0.05) at week 15. The combination of MTX with TWP had no significant effect on the content of total MTXPGs, short-chain MTXPGs and long-chain MTXPGs (P > 0.05), but it significantly decreased the content of very long-chain MTXPGs (P < 0.05) at week 15.

Conclusions

The UPLC-MS/MS method was successfully used to determine MTXPGs in rat erythrocytes. TGP and TWP in combination with MTX affected the production of MTXPGs of different chain lengths in erythrocytes.
The Zhenhua Case: the emergence of civil environmental public interest litigation in China
Authors: Yan Wenjun
Published: February 2021

In 2015, the All-China Environment Federation v Dezhou Jinghua Group Zhenhua Corporation Limited case was the first civil environmental public interest litigation (CEPIL) against air pollution in China. Constituting a milestone in the field of air pollution control in China, this case (i) confirms the eligibility of a non-governmental organisation (NGO) to file civil public interest litigations; (ii) discusses remedies for the ecological destruction caused by air pollution; (iii) assesses the ecological and environmental damage using the ‘virtual restoration cost’ method; and (iv) uses public apology as an innovative way for Zhenhua to assume liability. By applying and interpreting several important rules under the Environmental Protection Law of China (EPLC) for the first time, this case sets an example for future CEPILs against air pollution in China.
Ammonium supply induces differential metabolic adaptive responses in tomato according to leaf phenological stage
Authors: Poucet Théo , González-Moro María Begoña , Cabasson Cécile , Beauvoit Bertrand , Gibon Yves , Dieuaide-Noubhani Martine , Marino Daniel
Published: February 2021

Nitrate (NO3−) and ammonium (NH4+) are the main inorganic nitrogen sources available to plants. However, exclusive ammonium nutrition may lead to stress characterized by growth inhibition, generally associated with a profound metabolic reprogramming. In this work, we investigated how metabolism adapts according to leaf position in the vertical axis of tomato (Solanum lycopersicum cv. M82) plants grown with NH4+, NO3−, or NH4NO3 supply. We dissected leaf biomass composition and metabolism through an integrative analysis of metabolites, ions, and enzyme activities. Under ammonium nutrition, carbon and nitrogen metabolism were more perturbed in mature leaves than in young ones, overall suggesting a trade-off between NH4+ accumulation and assimilation to preserve young leaves from ammonium stress. Moreover, NH4+-fed plants exhibited changes in carbon partitioning, accumulating sugars and starch at the expense of organic acids, compared with plants supplied with NO3−. We explain such reallocation by the action of the biochemical pH-stat as a mechanism to compensate the differential proton production that depends on the nitrogen source provided. This work also underlines that the regulation of leaf primary metabolism is dependent on both leaf phenological stage and the nitrogen source provided.
Clinical Predictors of SARS-CoV-2 Testing Pressure on Clinical Laboratories: A Multinational Study Analyzing Google Trends and Over 100 Million Diagnostic Tests
Authors: Lippi Giuseppe , Mattiuzzi Camilla , Santos de Oliveira Maria Helena , Henry Brandon M
Published: February 2021

Objective

Evidence has shown that Google searches for clinical symptom keywords correlates with the number of new weekly patients with COVID-19. This multinational study assessed whether demand for SARS-CoV-2 tests could also be predicted by Google searches for key COVID-19 symptoms.

Methods

The weekly number of SARS-CoV-2 tests performed in Italy and the United States was retrieved from official sources. A concomitant electronic search was performed in Google Trends, using terms for key COVID-19 symptoms.

Results

The model that provided the highest coefficient of determination for the United States (R2 = 82.8%) included a combination of searching for cough (with a time lag of 2 weeks), fever (with a time lag of 2 weeks), and headache (with a time lag of 3 weeks; the time lag refers to the amount of time between when a search was conducted and when a test was administered). In Italy, headache provided the model with the highest adjusted R2 (86.8%), with time lags of both 1 and 2 weeks.

Conclusion

Weekly monitoring of Google Trends scores for nonspecific COVID-19 symptoms is a reliable approach for anticipating SARS-CoV-2 testing demands ~2 weeks in the future.
Multiplicity among the cool supergiants in the Magellanic Clouds
Authors: Dorda R , Patrick L R
Published: February 2021

The characterization of multiplicity of high-mass stars is of fundamental importance to understand their evolution, the diversity of observed core-collapse supernovae and the formation of gravitational wave progenitor systems. Despite that, until recently, one of the final phases of massive star evolution – the cool supergiant phase – has received comparatively little attention. In this study, we aim to explore the multiplicity among the cool supergiant (CSG) population in the Large and Small Magellanic Clouds (LMC and SMC, respectively). To do this we compile extensive archival radial velocity (RV) measurements for over 1000 CSGs from the LMC and SMC, spanning a baseline of over 40 yr. By statistically correcting the RV measurements of each stellar catalogue to the Gaia DR2 reference frame we are able to effectively compare these diverse observations. We identify 45 CSGs where RV variations cannot be explained through intrinsic variability, and are hence considered binary systems. We obtain a minimum binary fraction of $15\pm 4{{\ \rm per\ cent}}$ for the SMC and of $14\pm 5{{\ \rm per\ cent}}$ for the LMC, restricting our sample to objects with at least 6 and 5 observational epochs, respectively. Combining these results, we determine a minimum binary fraction of $15\pm 3{{\ \rm per\ cent}}$ for CSGs. These results are in good agreement with previous results which apply a correction to account for observational biases. These results add strength to the hypothesis that the binary fraction of CSGs is significantly lower than their main-sequence counterparts. Going forward, we stress the need for long-baseline multi-epoch spectroscopic surveys to cover the full parameter space of CSG binary systems.
The relationship between photometric and spectroscopic oscillation amplitudes from 3D stellar atmosphere simulations
Authors: Zhou Yixiao , Nordlander Thomas , Casagrande Luca , Joyce Meridith , Li Yaguang , Amarsi Anish M , Reggiani Henrique , Asplund Martin
Published: February 2021

We establish a quantitative relationship between photometric and spectroscopic detections of solar-like oscillations using ab initio, 3D, hydrodynamical numerical simulations of stellar atmospheres. We present a theoretical derivation as a proof of concept for our method. We perform realistic spectral line formation calculations to quantify the ratio between luminosity and radial velocity amplitude for two case studies: the Sun and the red giant ϵ Tau. Luminosity amplitudes are computed based on the bolometric flux predicted by 3D simulations with granulation background modelled the same way as asteroseismic observations. Radial velocity amplitudes are determined from the wavelength shift of synthesized spectral lines with methods closely resembling those used in Birmingham Solar Oscillations Network (BiSON) and Stellar Oscillations Network Group (SONG) observations. Consequently, the theoretical luminosity to radial velocity amplitude ratios are directly comparable with corresponding observations. For the Sun, we predict theoretical ratios of 21.0 and 23.7 ppm [m s−1]−1 from BiSON and SONG, respectively, in good agreement with observations 19.1 and 21.6 ppm [m s−1]−1. For ϵ Tau, we predict K2 and SONG ratios of 48.4 ppm [m s−1]−1, again in good agreement with observations 42.2 ppm [m s−1]−1, and much improved over the result from conventional empirical scaling relations that give 23.2 ppm [m s−1]−1. This study thus opens the path towards a quantitative understanding of solar-like oscillations, via detailed modelling of 3D stellar atmospheres.
White dwarf–main sequence star collisions from wide triples in the field
Authors: Michaely Erez , Shara Michael M
Published: February 2021

Multiple star systems interact strongly with galactic field stars when the outer semi-major axis of a triple or multiple star is >103 AU. Stable triples composed of two white-dwarfs (WD) and a low-mass main sequence (MS) star in a wide outer orbit can thus be destabilized by gravitational interactions with random field stars. Such interactions excite the eccentricity of the distant third star sufficiently so that it begins to interact significantly with the inner binary. When this occurs, the triple undergoes multiple binary-single resonant encounters. These encounters may result either in a collision between the non-degenerate component and a WD, or the breakup of the triple into a compact binary and a third object which is ejected. The compact binary can be either a MS–WD pair which survives, or collides or a double WD (DWD), which may inspiral through gravitational wave emission. We calculate the collision rate between a MS and WD star, and the merger rate of DWDs. Additionally, we describe the prospects of detectability of such a collision, which may resemble a sub-luminous supernovae event.
AlFoCS + F3D – II. Unexpectedly low gas-to-dust ratios in the Fornax galaxy cluster
Authors: Zabel Nikki , Davis Timothy A , Smith Matthew W L , Sarzi Marc , Loni Alessandro , Serra Paolo , Lara-López Maritza A , Cigan Phil , Baes Maarten , Bendo George J , De Looze Ilse , Iodice Enrichetta , Kleiner Dane , Koribalski Bärbel S , Peletier Reynier , Pinna Francesca , de Zeeuw P Tim
Published: February 2021

We combine observations from Atacama Large Millimeter/submillimeter Array (ALMA), Australia Telescope Compact Array, Multi Unit Spectroscopic Explorer (MUSE), and Herschel to study gas-to-dust ratios in 15 Fornax cluster galaxies detected in the FIR/sub-mm by Herschel and observed by ALMA as part of the ALMA Fornax Cluster Survey. The sample spans a stellar mass range of 8.3 ≤  log(M⋆/M⊙) ≤ 11.16, and a variety of morphological types. We use gas-phase metallicities derived from MUSE observations (from the Fornax3D survey) to study these ratios as a function of metallicity, and to study dust-to-metal ratios, in a sub-sample of nine galaxies. We find that gas-to-dust ratios in Fornax galaxies are systematically lower than those in field galaxies at fixed stellar mass/metallicity. This implies that a relatively large fraction of the metals in these Fornax systems is locked up in dust, which is possibly due to altered chemical evolution as a result of the dense environment. The low ratios are not only driven by H i deficiencies, but H2-to-dust ratios are also significantly decreased. This is different in the Virgo cluster, where low gas-to-dust ratios inside the virial radius are driven by low H i-to-dust ratios, while H2-to-dust ratios are increased. Resolved observations of NGC 1436 show a radial increase in H2-to-dust ratio, and show that low ratios are present throughout the disc. We propose various explanations for the low H2-to-dust ratios in the Fornax cluster, including the more efficient stripping of H2 compared to dust, more efficient enrichment of dust in the star formation process, and altered interstellar medium physics in the cluster environment.
Eigenstates of quasi-Keplerian self-gravitating particle discs
Authors: Melton Walker , Batygin Konstantin
Published: February 2021

Although quasi-Keplerian discs are among the most common astrophysical structures, computation of secular angular momentum transport within them routinely presents a considerable practical challenge. In this work, we investigate the secular small-inclination dynamics of a razor-thin particle disc as the continuum limit of a discrete Lagrange–Laplace secular perturbative theory and explore the analogy between the ensuing secular evolution – including non-local couplings of self-gravitating discs – and quantum mechanics. We find the ‘quantum’ Hamiltonian that describes the time evolution of the system and demonstrate the existence of a conserved inner product. The lowest-frequency normal modes are numerically approximated by performing a Wick rotation on the equations of motion. These modes are used to quantify the accuracy of a much simpler local-coupling model, revealing that it predicts the shape of the normal modes to a high degree of accuracy, especially in narrow annuli, even though it fails to predict their eigenfrequencies.
Distances to Galactic X-ray binaries with Gaia DR2
Authors: Arnason R M , Papei H , Barmby P , Bahramian A , D. Gorski M
Published: February 2021

Precise and accurate measurements of distances to Galactic X-ray binaries (XRBs) reduce uncertainties in the determination of XRB physical parameters. We have cross-matched the XRB catalogues of Liu, van Paradijs & van den Heuvel to the results of Gaia Data Release 2. We identify 86 XRBs with a Gaia candidate counterpart, of which 32 are low-mass X-ray binaries (LMXBs) and 54 are high-mass X-ray binaries (HMXBs). Distances to Gaia candidate counterparts are, on average, consistent with those measured by Hipparcos and radio parallaxes. When compared to distances measured by Gaia candidate counterparts, distances measured using Type I X-ray bursts are systematically larger, suggesting that these bursts reach only 50 per cent of the Eddington limit. However, these results are strongly dependent on the prior assumptions used for estimating distance from the Gaia parallax measurements. Comparing positions of Gaia candidate counterparts for XRBs in our sample to positions of spiral arms in the Milky Way, we find that HMXBs exhibit mild preference for being closer to spiral arms; LMXBs exhibit mild preference for being closer to interarm regions. LMXBs do not exhibit any preference for leading or trailing their closest spiral arm. HMXBs exhibit a mild preference for trailing their closest spiral arm. The lack of a strong correlation between HMXBs and spiral arms may be explained by star formation occurring closer to the mid-point of the arms, or a time delay between star formation and HMXB formation manifesting as a spatial separation between HMXBs and the spiral arm where they formed.
Ionic liquid-based electrolytes for CO2 electroreduction and CO2 electroorganic transformation
Authors: Tan Xingxing , Sun Xiaofu , Han Buxing
Published: February 2021

CO2 is an abundant and renewable C1 feedstock. Electrochemical transformation of CO2 can integrate CO2 fixation with renewable electricity storage, providing an avenue to close the anthropogenic carbon cycle. As a new type of green and chemically tailorable solvent, ionic liquids (ILs) have been proposed as highly promising alternatives for conventional electrolytes in electrochemical CO2 conversion. This review summarizes major advances in the electrochemical transformation of CO2 into value-added carbonic fuels and chemicals in IL-based media in the past several years. Both the direct CO2 electroreduction (CO2ER) and CO2-involved electroorganic transformation (CO2EOT) are discussed, focusing on the effect of electrocatalysts, IL components, reactor configurations and operating conditions on catalytic activity, selectivity and reusability. The reasons for the enhanced CO2 conversion performance by ILs are also discussed, providing guidance for the rational design of novel IL-based electrochemical processes for CO2 conversion. Finally, the critical challenges remaining in this research area and promising directions for future research are proposed.
An evaluation of REACTMH mental health training for healthcare supervisors
Authors: Akhanemhe R , Wallbank S , Greenberg N
Published: February 2021

Background

Healthcare workers (HCWs) are vulnerable to developing mental ill-health. Prior research has shown those in managerial or supervisory positions have pivotal roles in creating a healthy work environment, as well as recognizing and supporting HCWs with mental health symptoms to improve occupational outcomes and reduce sickness absence.

Aims

To evaluate the effectiveness of active listening skills training (REACTMH) for UK healthcare managers.

Methods

Managers of HCWs were voluntarily enrolled on a REACTMH active listening skills training package. Attendees anonymously completed questionnaires before and immediately after attending the 1-h remote training session and again 1 month later. The questionnaire asked about confidence in recognizing, conversing with and supporting distressed colleagues as well as perceived usefulness and importance of the training and how often they had used the taught active listening skills.

Results

Fifty-eight healthcare managers enrolled onto the REACTMH programme in July 2020 and 32 (55%) completed the 1-month follow-up. Just less than half reported feeling confident in being able to identify, speak to and support potentially distressed staff before the training; significantly more (>80%) reported feeling confident 1 month afterwards (P < 0.001). Around three-quarters of attendees reported the REACTMH programme as highly useful, highly important and said they had frequently used the learned skills.

Conclusions

A brief active listening skills training package was associated with significant improvements healthcare manager’s confidence in recognizing, speaking with and supporting HCWs with suspected mental health conditions. Further research is needed to investigate the longer term effectiveness and acceptability of REACTMH training.
Nasopharyngeal Panbio COVID-19 Antigen Performed at Point-of-Care Has a High Sensitivity in Symptomatic and Asymptomatic Patients With Higher Risk for Transmission and Older Age
Authors: Masiá Mar , Fernández-González Marta , Sánchez Manuel , Carvajal Mar , García José Alberto , Gonzalo-Jiménez Nieves , Ortiz de la Tabla Victoria , Agulló Vanesa , Candela Inmaculada , Guijarro Jorge , Gutiérrez José Antonio , de Gregorio Carlos , Gutiérrez Félix
Published: February 2021

Background

Performance of point-of-care tests in different clinical scenarios and on different samples remains undetermined. We comprehensively evaluated the performance of the nasopharyngeal Panbio COVID-19 Ag Rapid Test Device.

Methods

This is a prospective study that includes consecutive patients attending 3 primary care centers (PCCs) and an emergency department. The antigen test was performed at point-of-care in nasopharyngeal and nasal swabs and in saliva. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated with the reverse-transcription polymerase chain reaction (RT-PCR) assay as reference standard.

Results

Of 913 patients included, 296 (32.3%) were asymptomatic and 690 (75.6%) came from the PCC. Nasopharyngeal swabs were collected from 913 patients, nasal swabs were collected from 659 patients, and saliva was collected from 611 patients. The RT-PCR was positive in 196 (21.5%) nasopharyngeal samples (NPS). Overall, PPA (95% CI) in NPS was 60.5% (53.3–67.4), and it was lower in nasal swabs (44.7%) and saliva (23.1%). Test performance in NPS was largely dependent on the cycle threshold (Ct) in RT-PCR, with PPA of 94% for Ct ≤25 and 80% for Ct <30. In symptomatic patients, the PPA was 95% for Ct ≤25, 85% for Ct <30, and 89% for the symptom triad of fever, cough, and malaise. Performance was also dependent on age, with a PPA of 100% in symptomatic patients >50 years with Ct <25. In asymptomatic patients, the PPA was 86% for Ct <25. In all cases, NPA was 100%.

Conclusions

The nasopharyngeal Panbio COVID-19 Ag test performed at point-of-care has a good sensitivity in symptomatic patients with Ct <30 and older age. The test was useful to identify asymptomatic patients with lower Ct values.
Cephalopod paralarval species richness, abundance and size structure during the 2014–2017 anomalous warm period in the southern Gulf of California
Authors: Martínez-Soler Elizabeth , Gómez-Gutiérrez Jaime , de Silva-Dávila Roxana , González-Rodríguez Eduardo , Aburto-Oropeza Octavio
Published: February 2021

Cephalopod paralarval species richness, abundance and size structure were surveyed wduring an anomalous warm period (2014–2017) in the Cabo Pulmo National Park (CPNP), Gulf of California, Mexico. Paralarval taxa from six families (Cranchiidae, Enoploteuthidae, Ommastrephidae, Onychoteuthidae, Argonautidae and Octopodidae) were identified. Most taxa were of tropical biogeographic affinity and oceanic habitats. Highest paralarval richness occurred during spring associated with the northward movement of Tropical Surface Water, while the lowest was recorded during autumn with the southward flow of the Gulf of California water mass. Although 89% of the paralarvae were collected at or close to their species hatching size, none of the paralarval taxa showed a consistent seasonal spawning period. A canonical correspondence analysis showed three taxonomic assemblages: Argonauta and Helicocranchia–Onychoteuthis groups correlated with northward currents and high zooplankton biovolumes (ZB) and SD complex–Abraliopsis group with southward currents and intermediate ZB. Our Helicocranchia pfefferi paralarvae are the first recorded for the Gulf of California. At least 11 cephalopod taxa reproduce in CPNP. This area represents a suitable spawning habitat for cephalopods of socio-economic value.
Container volume may affect growth rates of ciliates and clearance rates of their microcrustacean predators in microcosm experiments
Authors: Weisse Thomas , Lukić Dunja , Lu Xiaoteng
Published: February 2021

We studied the effect of volume in small containers (microcosms) on five common planktonic freshwater ciliates and three zooplankton species, namely Daphnia sp., the calanoid copepod Eudiaptomus sp., and the cyclopoid copepod Cyclops sp. We measured ciliate specific growth rates and their loss rates due to microcrustacean predation in short-term experiments. We hypothesized that container volume ranging from 10 to 200 mL would not affect the activity of our prey and predator species. We found that the response to volume was species-specific; growth rates of three ciliate species were sensitive to volume. However, the volume effect was not unequivocal because different timing of the microcosm experiments (block effects) may have caused random bias due to varying morphological and/or physiological conditions of the ciliates and their predators. For predator clearance rate, the volume effect was insignificant in the filter-feeding Daphnia and Eudiaptomus but was significant for the predatory copepod Cyclops, which was hampered in the smallest experimental containers. Total crustacean clearance rates averaged over all treatments appeared unaffected by predator species, while ciliate species significantly affected the results. Our growth and clearance rates are close to previous findings with the same or similar planktonic prey and predator species.
Estimating the population health burden of musculoskeletal conditions using primary care electronic health records
Authors: Yu Dahai , Peat George , Jordan Kelvin P , Bailey James , Prieto-Alhambra Daniel , Robinson Danielle E , Strauss Victoria Y , Walker-Bone Karen , Silman Alan , Mamas Mamas , Blackburn Steven , Dent Stephen , Dunn Kate , Judge Andrew , Protheroe Joanne , Wilkie Ross
Published: February 2021

Objectives

Better indicators from affordable, sustainable data sources are needed to monitor population burden of musculoskeletal conditions. We propose five indicators of musculoskeletal health and assessed if routinely available primary care electronic health records (EHR) can estimate population levels in musculoskeletal consulters.

Methods

We collected validated patient-reported measures of pain experience, function and health status through a local survey of adults (≥35 years) presenting to English general practices over 12 months for low back pain, shoulder pain, osteoarthritis and other regional musculoskeletal disorders. Using EHR data we derived and validated models for estimating population levels of five self-reported indicators: prevalence of high impact chronic pain, overall musculoskeletal health (based on Musculoskeletal Health Questionnaire), quality of life (based on EuroQoL health utility measure), and prevalence of moderate-to-severe low back pain and moderate-to-severe shoulder pain. We applied models to a national EHR database (Clinical Practice Research Datalink) to obtain national estimates of each indicator for three successive years.

Results

The optimal models included recorded demographics, deprivation, consultation frequency, analgesic and antidepressant prescriptions, and multimorbidity. Applying models to national EHR, we estimated that 31.9% of adults (≥35 years) presenting with non-inflammatory musculoskeletal disorders in England in 2016/17 experienced high impact chronic pain. Estimated population health levels were worse in women, older aged and those in the most deprived neighbourhoods, and changed little over 3 years.

Conclusion

National and subnational estimates for a range of subjective indicators of non-inflammatory musculoskeletal health conditions can be obtained using information from routine electronic health records.
What is the meaning of ANCA positivity in IgG4-related disease?
Authors: Martín-Nares Eduardo , Hernandez-Molina Gabriela
Published: February 2021

Objectives

To evaluate the prevalence and meaning of antineutrophil cytoplasmic antibodies (ANCA) positivity in a cohort of IgG4-related disease (IgG4-RD).

Methods

We identified patients with ANCA determination from a retrospective cohort of 69 patients with IgG4-RD. ANCA were measured by indirect immunofluorescence microscopy (IIF) and/or proteinase 3 (PR3)-ANCA and MPO-ANCA by ELISA. IIF patterns were classified as perinuclear (P-ANCA), cytoplasmic (C-ANCA) and atypical (X-ANCA). We compared the ANCA-positive vs the ANCA-negative IgG4-RD group.

Results

Out of 69 patients, 31 IgG4-RD patients had an ANCA determination. Four patients with concomitant systemic autoimmune diseases were excluded. We found positive ANCA by IIF in 14 (56%) of 25 patients tested. The most common IIF pattern was C-ANCA in eight (57.1%), followed by dual C-ANCA/X-ANCA in four (28.6%) and P-ANCA and dual C-ANCA/P-ANCA in one each (7.1%). Of the 20 patients with ANCA determination by both IIF and ELISA, four have positive ANCA by ELISA (three for MPO-ANCA and one for PR3-ANCA). Of the two patients with only ELISA determination, one was positive for MPO-ANCA. The prevalence of ANCA positivity by ELISA was 22.7% (5 out of 22 patients). ANCA was more frequent in the Mikulizc/systemic phenotype (42.9%) compared with other phenotypes (P = 0.04). ANCA-positive IgG4-RD patients had more frequently lymph node and kidney involvement, high IgG1 levels and erythrocyte sedimentation rate, and positive antinuclear antibodies.

Conclusion

ANCA are found in a significant number of patients with IgG4-RD and differed from the ANCA-negative group in terms of clinical and serological features.
MINIMIZING THE EXPOSURE TO THE EYE LENS OF RADIOLOGIC TECHNOLOGISTS ASSISTING PATIENTS DURING CHEST X RAYS: A PHANTOM STUDY
Authors: Mitsuyoshi Yasuda , Tomoya Funada , Hisaya Sato , Kyoichi Kato
Published: February 2021

As chest x rays involve risks of patients falling, radiologic technologists (technologists) commonly assist patients, and as the assistance takes place near the patients, the eye lenses of the technologists are exposed to radiation. The recommendations of the International Commission on Radiological Protection suggest that the risk of developing cataracts due to lens exposure is high, and this makes it necessary to reduce and minimize the exposure. The present study investigated the positions of technologists assisting patients that will minimize exposure of the eye lens to radiation. The results showed that it is possible to reduce the exposure by assisting from the following positions: 50% at the sides rather than diagonally behind, 10% at the right side of the patient rather than the left and 40% at 250 mm away from the patient. The maximum reduction with radiation protection glasses was 54% with 0.07 mmPb and 72% with 0.88 mmPb.
Physical Activity Interventions for Individuals With a Mild Traumatic Brain Injury:
Authors: Alarie Christophe , Gagnon Isabelle , Quilico Enrico , Teel Elizabeth , Swaine Bonnie
Published: February 2021

Objective:

To document the characteristics, measured outcomes, and effectiveness of physical activity (PA) interventions designed to improve health-related outcomes in individuals with a mild traumatic brain injury (mTBI) to assist in rehabilitation quality improvement efforts of a TBI rehabilitation program.

Methods:

A scoping review following a 6-step iterative framework search across 5 databases (MEDLINE, CINAHL, PsycINFO, SPORTDiscuss, and EMBASE) and the gray literature (Google) was performed. Selected PA interventions were designed for individuals of all ages and any mechanism of injury (eg, sports-related and falls). Data were charted, collated, and summarized according to the Consensus on Exercise Reporting Template checklist and domains of the International Classification of Functioning, Disability and Health. Involvement of clinical experts ensured tailoring of the knowledge synthesis to meet clinical needs.

Results:

Thirty-five articles and 14 gray literature records were retained. Five types of PA interventions were identified with the majority being multimodal. Reporting of PA intervention characteristics was highly variable across studies; many details necessary for intervention replication are missing. Study outcomes focused primarily on improving body functions and symptoms of mTBI, and less frequently on activities, participation, and health-related quality of life. The methodological quality of studies varies.

Conclusions:

Identified PA intervention types offer various management options for healthcare providers. PA interventions may improve a wide range of health-related outcomes supporting the inclusion of PA in the management of individuals of all ages with mTBI. Higher-quality research and better reporting about intervention characteristics is however needed.
Efficacy of Ivermectin in COVID-19 Patients with Mild to Moderate Disease
Authors: Shah Bukhari Karamat Hussain , Asghar Asma , Perveen Najma , Hayat Arshad , Mangat Sermad Ahmad , Butt Kamil Rehman , Abdullah Mohammad , Fatima Tehreem , Mustafa Ahmad , Iqbal Talal
Published: February 2021

Objective

To evaluate the efficacy of ivermectin (IVM) as an addition to the standard of care (SOC) treatment in COVID-19 patients with mild and moderate disease

Materials and Methods

A randomized clinical trial (Trial registration # NCT04392713) was carried out at Combined Military Hospital Lahore from March 15, 2020, to June 15, 2020. Eighty-six patients with reverse transcriptase-polymerase chain reaction (RT-PCR) proven SARS-CoV-2 infection completed the trial protocol. Patients were stratified via the lottery method into two groups. Group A was administered standard of care (SOC) treatment as per existing hospital guidelines whereas group B was given ivermectin (single dose of 12 milligrams) along with SOC treatment. PCR was repeated at 72 hours, 7th day, and at 14th day of admission for both the groups and the point at which the PCR became negative was noted. Complete blood counts, liver function tests and renal function tests were done at recruitment, 7th day, and 14th day. The primary outcome was the viral clearance, measured as days to achieve PCR negativity. The secondary outcome was the development of any adverse side effects pertinent to ivermectin or derangement in baseline laboratory parameters.

Results

In group A, 36 (80%) participants were males, and 9 (20%) were females, whereas in group B, 37 (90.2%) were males and 4 (9.8%) were females. Mean age was 39.0± 12.6 and 42.2 ± 12.0 years for groups A and B, respectively (p= 0.394). There was early viral clearance in group B as compared to group A (p=0.001). No adverse reaction or derangements in laboratory parameters was noted in the intervention arm during the trial period.

Conclusion

In the intervention arm, early viral clearance was observed and no side effects were documented. Therefore ivermectin is a potential addition to the standard care of treatment in COVID-19 patients.
Thromboembolic risk in hospitalised and non-hospitalised Covid-19 patients: A self-controlled case series analysis of a nation-wide cohort
Authors: Ho Frederick K , Man Kenneth KS , Toshner Mark , Church Colin , Celis-Morales Carlos , Wong Ian CK , Berry Colin , Sattar Naveed , Pell Jill P
Published: February 2021

Objective

An unexpectedly large number of people infected with Covid-19 had experienced a thrombotic event. This study aims to assess the associations between Covid-19 infection and thromboembolism including myocardial infarction (MI), ischaemic stroke, deep-vein thrombosis (DVT), and pulmonary embolism (PE).

Patients and Methods

A self-controlled case-series study was conducted covering the whole of Scotland’s general population. The study population comprised individuals with confirmed (positive test) Covid-19 and at least one thromboembolic event between March 2018 and October 2020. Their incidence rates during the risk interval (5 days before to 56 days after the positive test) and the control interval (the remaining periods) were compared intra-personally.

Results

Across Scotland, 1,449 individuals tested positive for Covid-19 and experienced a thromboembolic event. The risk of thromboembolism was significantly elevated over the whole risk period but highest in the 7 days following the positive test (IRR 12.01, 95% CI 9.91-14.56) in all included individuals. The association was also present in individuals not originally hospitalised for Covid-19 (IRR 4.07, 95% CI 2.83-5.85). Risk of MI, stroke, PE and DVT were all significantly higher in the week following a positive test. The risk of PE and DVT was particularly high and remained significantly elevated even 56 days following the test.

Conclusion

Confirmed Covid-19 infection was associated with early elevations in risk with MI, ischaemic stroke, and substantially stronger and prolonged elevations with DVT and PE both in hospital and community settings. Clinicians should consider thromboembolism, especially PE, among people with Covid-19 in the community.
Effectiveness of early versus delayed rehabilitation following rotator cuff repair: systematic review and meta-analyses
Authors: Mazuquin Bruno , Moffatt Maria , Gill Peter , Selfe James , Rees Jonathan , Drew Steve , Littlewood Chris
Published: February 2021

Objective

To investigate the effectiveness of early rehabilitation compared with delayed/standard rehabilitation after rotator cuff repair for pain, function, range of movement, strength, and repair integrity.

Design

Systematic review and meta-analyses.

Methods

We searched databases and included randomised controlled trials (RCTs) comparing early with delayed/standard rehabilitation for patients undergoing rotator cuff repair surgery. We assessed risk of bias of the RCTs using the Cochrane RoB 2 tool.

Results

Twenty RCTs, with 1841 patients, were included. The majority of the RCTs were of high or unclear risk of overall bias. We found substantial variations in the rehabilitation programmes, time in the sling and timing of exercise progression. We found no statistically significant differences for pain and function at any follow-up except for the outcome measure Single Assessment Numeric Evaluation at six months (MD: 6.54; 95%CI: 2.24-10.84) in favour of early rehabilitation. We found statistically significant differences in favour of early rehabilitation for shoulder flexion at six weeks (MD: 7.36; 95%CI: 2.66-12.06), three (MD: 8.45; 95%CI: 3.43-13.47) and six months (MD: 3.57; 95%CI: 0.81-6.32) and one year (MD: 1.42; 95%CI: 0.21-2.64) and similar differences for other planes of movement. In terms of repair integrity at one year, regarding early mobilisation, the number needed to harm was 651.

Discussion

Current approaches to early mobilisation, based largely on early introduction of passive movement, did not demonstrate significant differences in most clinical outcomes, although we found statistically significant differences in favour of early rehabilitation for range of movement. Importantly, there were no differences in repair integrity between early and delayed/standard rehabilitation. Most rehabilitation programmes did not consider early active movement as soon as the patient feels able. With ongoing variation in rehabilitation protocols there remains a need for large high quality RCT to inform the optimal approach to rehabilitation after rotator cuff repair surgery.
ACTIVE SURVEILLANCE OF THE SPUTNIK V VACCINE IN HEALTH WORKERS
Authors: Pagotto Vanina , Ferloni Analia , Soriano María Mercedes , Díaz Morena , Golde Manuel Braguisnky , González María Isabel , Asprea Valeria , Staneloni Inés , Vidal Gustavo , Silveira Martin , Zingoni Paula , Aliperti Valeria , Michelangelo Hernan , Figar Silvana
Published: February 2021

Introduction

The World Health Organization (WHO) recommends vaccination against Sars Cov-2 coronavirus to mitigate COVID-19 pandemic. On December 29th, the Argentine Ministry of Health started a vaccination plan with the Sputnik V vaccine emphasizing the registration of the Events Supposedly Attributed to Vaccines and Immunizations (ESAVI) in the National Surveillance System. The aim of this study is to determine the safety of this vaccine.

Methods

In an ongoing cohort study, health professionals from Hospital Italiano de Buenos Aires vaccinated with the first component of the Sputnik V vaccine (a rAd26 vector-based) were followed up. Safety at 72 hs was analysed from a self-report form. Local and systemic reactions were characterized as mild, moderate and severe. Incident rates were calculated per 1000 person-hours by age groups and gender. Adjusted hazard ratio and 95% Confidence Interval (HR; 95%CI) is obtained by Cox Regression Model.

Results

707 health professionals (mean age 35, 67% female) were vaccinated, response rate was 96,6% and 71,3% reported at least one ESAVI. Rate was 6.3 per 1.000 person-hours. Among local reactions, 54% reported pain at the injection site, 11% redness and swelling. Among systemic reactions 40% reported fever, 5% diarrhea and 68% new or worsened muscle pain. Five percent had serious adverse events that required medical evaluation and one inpatient.

ESAVI rate was higher among females (65.4% vs 50%; HR 1.38, 95%CI 1.13-5.38) and in younger than 55 years-old (72.8% vs 32%; HR 2.66, 95%CI 1.32-1.68).

Conclusion

Active surveillance on safety for vaccines with emergency approval is mandatory. This study shows high rates of local and systemic reactions however early serious events were rare. Short term safety is supported by these preliminary findings. Studies on long term safety and efficacy, accoding sex and age, are needed.