bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Mind Mapping Learning on Learning Interests Social Studies Fifth Grade
Authors: RiskhaYeni Refi , Raharja Hawwin Fitra , Salamah Evi Rizqi
Published: April 2024

Formal education at elementary school level appears several Problems related to social studies learning. The problems which often happened during social studies is about the standard of process. The goal of this research is to find out the interest of learning on social studies in type of economic action and in Indonesia on fifth grade students of Jombang before and after using mind mapping learning model. Also to understand any difference of mind mapping model. This research use quantitative approach by using pre-Experiental design method with one group pre-test and post-test design. Data collecting in this research in multiple choices question pre-test, post test and documentation.Based on t-test calculations which already decided based on pre-test and post-test result with t-test paired sample test then obtained t-calculate acquired value 3,341 and t-table is 2,093 and significant value (2 tailed) is 0,000 < 0,05 So result of t-calculate > t-table which means H accepted and H rejected. So, it can assumed that there is the effect of learning interest of social studies on fifth grade of Jatipelem state elementary school I diwelk, Jombang by using mind mapping learning model.
LIFE: A metric for quantitively mapping the impact of land-cover change on global extinctions
Authors: Eyres Alison , Ball Thomas , Dales Michael , Swinfield Tom , Arnell Andy , Baisero Daniele , Durán América Paz , Green Jonathan , Green Rhys E , Madhavapeddy Anil , Balmford Andrew
Published: April 2024

Human-driven habitat loss is recognised as the greatest cause of biodiversity loss yet to date we lack robust, spatially explicit metrics quantifying the impacts of anthropogenic changes in habitat extent on species’ extinctions. Existing metrics either fail to consider species identity or focus solely on recent habitat losses. The persistence score approach developed by Durán et al. (2020) (1) represented an important development by combining species’ ecologies and land-cover data whilst considering the cumulative and non-linear impact of past habitat loss on species’ probability of extinction. However, it is computationally demanding, limiting its global use and application. Here we couple the persistence score approach with high-performance computing to generate global maps of what we term the LIFE (Land-cover change Impacts on Future Extinctions) metric for 29772 species of terrestrial vertebrates at 1 arc-minute resolution (3.4km2 at the equator). These maps provide quantitative estimates, for the first time, of the marginal changes in the expected number of extinctions (both increases and decreases) caused by (1) converting remaining natural vegetation to agriculture, and (2) restoring farmland to natural habitat. We demonstrate statistically that this approach integrates information on species richness, endemism, and past habitat loss. Our resulting maps can be used to 10% certainty at scales from 0.5-1000km2, and offer unprecedented opportunities to estimate the impact on extinctions of diverse actions that change land cover, from individual dietary choices through to global protected area development.
Functional composition and generalization of natural numbers by decomposition of semiring structure
Authors: Sebata Junya
Published: April 2024

We improve semiring composition of natural numbers to functional composition of natural numbers. From this functional composition, we select one generalization of natural numbers and see a specificity of natural numbers with proving a combinatorial theorem which derives fundamental identities.
Experimental Techniques for the Characterization of Magnetic Thin Films
Authors: Singh Amit Kumar , Kumar Ashwani
Published: April 2024

This review article describes the various experimental techniques, which are required for the characterization of magnetic thin films. Characterization is an essential process to understand the properties of magnetic thin films. By knowing the properties of these materials, one can utilize them in various technological applications as per requirements. There are different characterization tools to study the different properties of nanostructured materials. Some important experimental techniques employed to characterize the magnetic thin films are: X-Ray Diffraction, X-Ray reflectivity, Field emission scanning electron microscopy, Energy dispersive spectrometer, Transmission electron microscopy, Superconducting quantum interference device, Raman spectroscopy and X-ray absorption spectroscopy etc. These techniques will develop a better understanding of the structural, morphological, and magnetic properties of the materials to the scientific community. The discussion made in the present review article would be extremely helpful to the researchers who are doing research in the various branches of science and engineering.
Robustness, redundancy, inclusivity, and integration of built environment systems: resilience quantification from stakeholders’ perspectives
Authors: Al-Humaiqani Mohammed M. , Al-Ghamdi Sami G.
Published: April 2024

The built environment faces a growing number of challenges due to changing climates. A resilient built environment system (BES) can withstand disruptions and shocks, and resilient design allows communities to bounce back quickly. Considering present and future needs, BESs can be oriented to adapt to new uses or modified to handle changing climates. This study examines the resilience qualities (RQs) of built environment systems (BESs) in responding to and recovering from climate change disruptions effectively. A survey was designed to capture the views of various stakeholders about the different indicators to assess the four RQs: robustness (Rb), redundancy (Rd), inclusivity (Ic), and integration (It). Regulatory and engineering stakeholders participated in the survey, and the results were analyzed using statistical methods. Stakeholders generally agree on the need to enhance transformative capacity for addressing uncertainties and climate challenges. While stakeholders trust the role of BESs’ robustness against climate impacts, some suggest improving standards for better resilience. There is consensus on the importance of regulatory measures mandating emergency resources in BESs. The study highlights the need to enhance adaptive capacities and tools within BESs. Incorporating reconfigurability and spare capacity in BESs is crucial to prevent disruptions. Participants tend to think promoting good practices at the community level is essential to address climate impacts effectively. The analysis highlights the importance of inclusive community consultation and involvement in fostering a shared responsibility for enhancing urban ecosystems against climate change impacts. This involves aligning processes across various city systems to support cohesive decision-making and strategic investments. The study suggests developing objective engineering techniques to establish a standardized approach for evaluating the RQs of BESs.
Isolation of mucosa-associated microbiota dysbiosis in the ascending colon in hepatitis C virus post-sustained virologic response cirrhotic patients
Authors: Midori Yohei , Nosaka Takuto , Hiramatsu Katsushi , Akazawa Yu , Tanaka Tomoko , Takahashi Kazuto , Naito Tatsushi , Matsuda Hidetaka , Ohtani Masahiro , Nakamoto Yasunari
Published: April 2024

Background

Achieving sustained virologic response (SVR) in patients infected with hepatitis C virus (HCV) reduces all-cause mortality. However, the mechanisms and risk factors for liver fibrosis and portal hypertension post-SVR remain incompletely understood. In the gut-liver axis, mucosa-associated microbiota (MAM) substantially influence immune and metabolic functions, displaying spatial heterogeneity at the anatomical intestinal site. We analyzed MAM composition and function to isolate the locoregional MAM involved in chronic liver disease progression in HCV post-SVR patients.

Methods

We collected MAM samples from three intestinal sites (terminal ileum, ascending colon, and sigmoid colon) via brushing during colonoscopy in 23 HCV post-SVR patients and 25 individuals without liver disease (controls). The 16S rRNA of bacterial DNA in specimens collected with a brush and in feces was sequenced. The molecular expression of intestinal tissues and hepatic tissues were evaluated by quantitative real-time PCR.

Results

In the post-SVR group, the microbial β-diversity of MAM, especially in the ascending colon, differed from the control group and was associated with liver fibrosis progression. In PICRUSt analysis, MAM in the ascending colon in the liver cirrhosis (LC) group showed compromised functions associated with the intestinal barrier and bile acid production, and FGF19 expression was markedly decreased in the terminal ileum biopsy tissue in the LC group. At the genus level, six short-chain fatty acid (SCFA)-producing bacterial genera, Blautia, Alistipes, Roseburia, Agathobaculum, Dorea, and Pseudoflavonifractor were reduced in the ascending colon of post-SVR LC patients.

Conclusion

In patients of HCV post-SVR, we identified the association between the degree of liver fibrosis and dysbiosis of mucosa-associated SCFA-producing bacterial genera that may be related to intestinal barrier and bile acid production in the ascending colon.
CHD-CXR: a de-identified publicly available dataset of chest x-ray for congenital heart disease
Authors: Zhixin Li , Gang Luo , Zhixian Ji , Sibao Wang , Silin Pan
Published: April 2024

Congenital heart disease is a prevalent birth defect, accounting for approximately one-third of major birth defects. The challenge lies in early detection, especially in underdeveloped medical regions where a shortage of specialized physicians often leads to oversight. While standardized chest x-rays can assist in diagnosis and treatment, their effectiveness is limited by subtle cardiac manifestations. However, the emergence of deep learning in computer vision has paved the way for detecting subtle changes in chest x-rays, such as lung vessel density, enabling the detection of congenital heart disease in children. This highlights the need for further investigation. The lack of expert-annotated, high-quality medical image datasets hinders the progress of medical image artificial intelligence. In response, we have released a dataset containing 828 DICOM chest x-ray files from children with diagnosed congenital heart disease, alongside corresponding cardiac ultrasound reports. This dataset emphasizes complex structural characteristics, facilitating the transition from machine learning to machine teaching in deep learning. To ascertain the dataset's applicability, we trained a preliminary model and achieved an area under the receiver operating characteristic curve (ROC 0.85). We provide detailed introductions and publicly available datasets at: https://www.kaggle.com/competitions/congenital-heart-disease.
Research progress on the improvement of cardiovascular diseases through the autonomic nervous system regulation of the NLRP3 inflammasome pathway
Authors: Hu Yuchi , Dai Songyuan , Zhao Lulu , Zhao Ling
Published: April 2024

Cardiovascular disease stands as a leading global cause of mortality. Nucleotide-binding Oligomerization Domain-like Receptor Protein 3 (NLRP3) inflammasome is widely acknowledged as pivotal factor in specific cardiovascular disease progression, such as myocardial infarction, heart failure. Recent investigations underscore a close interconnection between autonomic nervous system (ANS) dysfunction and cardiac inflammation. It has been substantiated that sympathetic nervous system activation and vagus nerve stimulation (VNS) assumes critical roles withinNLRP3 inflammasome pathway regulation, thereby contributing to the amelioration of cardiac injury and enhancement of prognosis in heart diseases. This article reviews the nexus between NLRP3 inflammasome and cardiovascular disorders, elucidating the modulatory functions of the sympathetic and vagus nerves within the ANS with regard to NLRP3 inflammasome. Furthermore, it delves into the potential therapeutic utility of NLRP3 inflammasome to be targeted by VNS. This review serves as a valuable reference for further exploration into the potential mechanisms underlying VNS in the modulation of NLRP3 inflammasome.
The (misleading) role of animal models in drug development
Authors: Hartung Thomas
Published: April 2024

Animals like mice and rats have long been used in medical research to help understand disease and test potential new treatments before human trials. However, while animal studies have contributed to important advances, too much reliance on animal models can also mislead drug development. This article explains for a general audience how animal research is used to develop new medicines, its benefits and limitations, and how more accurate and humane techniques—alternatives to animal testing—could improve this process.
Construction of street tree risk assessment system and empirical analysis based on non-destructive testing technologies
Authors: He Kun , Wei Longlong , Wang Benyao
Published: April 2024

The traditional visual tree assessment method is subjective in evaluating tree risks and therefore not effective in precisely detecting internal decay in tree trunk and root systems. To improve the accuracy of street tree risk assessment, a new nondestructive testing method was proposed. This new tree risk assessment method combines different non-destructive testing technologies, such as sonic tomography and ground-penetrating radar, which could significantly increase the accuracy of risk assessment in tree trunks and roots. The method was applied to evaluate the risk of 1,001 street trees in Shanghai’s historical feature protection area. The results revealed that despite most street trees having low branch and trunk risk levels, more than one-third had high root risk levels. The risk factors of street trees were mainly in the trunk and root system, with a significant correlation between the street tree risk level and tree cavities, diseases, and insect pests, as well as the depth and range of the root distribution, leaning, and internal decay in trunks. With the help of non-destructive testing and risk assessment analysis, as well as targeted prevention measures, the possibility of street risk damage was largely reduced, including street trees tilting and collapsing during typhoons, etc.
Assessing climate impacts on slow-moving landslides in the western Alps of Piemonte: integration of monitoring techniques for detecting displacements
Authors: Narcisi Roberta , Pappalardo Salvatore Eugenio , Taddia Glenda , De Marchi Massimo
Published: April 2024

The influence of climatic factors on landslides triggers and displacement rates is a crucial research topic, especially due to the growing need to understand the evolution of climate change in historical periods of intense precipitation and anomalous temperature increases. Italy, highly prone to hydrogeological instability, extremely its mountainous regions such as the Alps, stands as a pertinent subject area for instability scenarios. However, the interpretation of climate effects on landslides is still an open issue. This work proposed a simplified methodology for investigating the displacements of three slow-moving landslides located in the Western Alps of Piemonte region, in response to significant meteorological events evaluated from reference normal of precipitation and temperature trends over the reference period 1991–2020. Another purpose is to emphasize the advantages of using different monitoring techniques by comparing displacement time series measured with in situ and remote sensing instruments, to detect ground deformation processes of these gravitational phenomena. The existence of a robust monitoring network, coupled with InSAR dataset support, has allowed detecting climatic factors’ impact on displacement rates for the outlined case studies. The results have demonstrated the relationships between the identified climatic events and variations in displacement time series, as well as the potential of integrating field observations and InSAR techniques to improve the interpretation of landslide dynamics. Although this study has laid the basis for understanding the influence of climatic factors on landslide displacements, there is still much to investigate and refine. The proposed preliminary analysis will further improve the ability to predict, monitor and mitigate landslide risk under changing climate conditions.
Mental health in times of pandemic from the perspective of professors and students
Authors: Campos Juliana Alvares Duarte Bonini , Campos Lucas Arrais , Azevedo Maria Antonia Ramos , Martins Bianca Gonzalez , Silva Bianca Núbia Souza , Marôco João
Published: April 2024

Introduction

The COVID-19 pandemic brought profound societal changes and disruptions, including in the education system, which underwent swift modifications. It presented unique challenges for both professors and students, contributing to an increase in the prevalence of mental health-related symptoms.

Objective

To determine the prevalence of mental health disorders symptoms, coping strategies, and concerns among students (≥18 years) and professors at different times during the COVID-19 pandemic.

Methods

This was an online cross-sectional study (Student: N = 6,609; Phase 1 = 3,325; Phase 2 = 1,402; Phase 3 = 1,882; Professor: N = 9,096; Phase 1 = 3,924; Phase 2 = 2,223; Phase 3 = 2,949). Depression, Anxiety, and Stress Scale (DASS-21), Impact of Event Scale-revised (IES-R), and BriefCOPE inventory were used. The probability of presenting the symptoms was calculated by multiple logistic regression and odds ratio (OR).

Results

The prevalence of depression, anxiety, stress, and distress symptoms among students and professors was high (≥59.5% and ≥ 33.5%, respectively). Students, women, and those diagnosed with a mental disorder were more likely to have symptoms or distress. In the face of the pandemic, professors used more adaptive coping strategies than students. Health was the focus of professors’ concerns, while for students, future and labor market uncertainty were concerns derived from the health core.

Conclusion

The results point to the need to strengthen psychosocial support for both professors and students.
Online or face-to-face problem-based learning tutorials? Comparing perceptions and preferences of students and tutors
Authors: Atwa Hani Salem , Nasr El-Din Wael Amin , Kumar Archana Prabu , Potu Bhagath Kumar , Tayem Yasin Ibrahim , Al-Ansari Ahmed Mohamed , Deifalla Abdelhalim Salem , Shehata Mohamed Hany
Published: April 2024

Introduction

Problem-based learning (PBL) tutorials are recognized as an effective strategy for building clinical and research skills in modern-day medical education. Despite the wide adoption of this instructional strategy, worldwide research findings reported noticeable differences in the perceptions towards online versus face-to-face modes of PBL conduction among students and their tutors.

Methods

This cross-sectional analytical study was conducted at the College of Medicine and Medical Sciences, Arabian Gulf University (CMMS-AGU), Kingdom of Bahrain, during the academic year 2022-2023. The study examined the differences in perceptions and preferences among students and tutors regarding online and face-to-face modes of PBL tutorials. The study also compared the performance of 3rdand 4thyear medical students who were exposed to PBL tutorials through both modes.

Results

The performance of students in tutorials in both modes revealed no statistically significant difference. The study found that tutors reported more positive perception toward the face-to-face mode compared to the online mode of conducting PBL tutorials than students, while students reported more positive perception toward the online mode compared to the face-to-face mode of conducting PBL tutorials than tutors. Several advantages and challenges of both modes were recounted by the students and tutors.

Conclusion

Our study concludes that students were in favor of the online mode of conducting PBL sessions, while tutors were in favor of the face-to-face mode. Comparable performance of students in PBL tutorials implies that the online mode of conducting PBL tutorials might be as effective as the face-to-face mode for meeting teaching objectives and students’ learning outcomes. The difference between perceptions of students and faculty can be attributed to multiple factors including generation difference, previous experiences, and digital literacy. It is recommended that faculty receive proper training for effectively adopting online modes of learning.
Elevated sperm DNA fragmentation is correlated with an increased chromosomal aneuploidy rate of miscarried conceptus in women of advanced age undergoing fresh embryo transfer cycle
Authors: Fu Wanting , Cui Qiuying , Bu Zhiqin , Shi Hao , Yang Qingling , Hu Linli
Published: April 2024

Background

Male sperm DNA fragmentation (SDF) may be associated with assisted reproductive technology (ART) outcomes, but the impact of SDF on the occurrence of aneuploid-related miscarriage remains controversial.

Methods

Genome-wide single-nucleotide polymorphism-based chromosomal microarray analysis was performed on 495 miscarried chorionic villus samples undergone IVF/ICSI treatment from the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University. SDF was assessed using sperm chromatin structure assay. Patients were divided into four groups according to embryo transfer cycle type and maternal age, and the correlation between SDF and chromosome aberration was analyzed. A receiver operating characteristic (ROC) curve was utilized to find the optimal threshold.

Results

Total chromosomal aneuploidy rate was 54.95%, and trisomy was the most common abnormality (71.32%). The chromosomally abnormal group had higher SDF than the normal group (11.42% [6.82%, 16.54%] vs. 12.95% [9.61%, 20.58%], P = 0.032). After grouping, elevated SDF was significantly correlated with an increasing chromosome aneuploidy rate only in women of advanced age who underwent fresh embryo transfer (adjusted odds ratio:1.14 [1.00–1.29], adjusted-P = 0.045). The receiver operating characteristic curve showed that SDF can predict the occurrence of chromosomal abnormality of miscarried conceptus in this group ((area under the curve = 0.76 [0.60–0.91], P = 0.005), and 8.5% was the optimum threshold. When SDF was ≥ 8.5%, the risk of such patients increased by 5.76 times (adjusted odds ratio: 6.76 [1.20–37.99], adjusted-P = 0.030).

Conclusion

For women of advanced maternal age undergoing fresh embryo transfer, older oocytes fertilized using sperm with high SDF in IVF/ICSI treatment might increase the risk of chromosomal abnormality in miscarried conceptus.
Free triiodothyronine to free thyroxine ratio as a marker of poor prognosis in euthyroid patients with acute coronary syndrome and diabetes after percutaneous coronary intervention
Authors: Wang Shen , Wang Yue , Sun Shuaifeng , Li Fadong , Zhao Wenxin , Li Xinjian , Ye Maomao , Niu Yufei , Wu Xiaofan
Published: April 2024

Objectives

In recent years, the free triiodothyronine/free thyroxine (FT3/FT4) ratio, a new comprehensive index for evaluating thyroid function, which could reflect thyroid function more stably and truly than serum thyroid hormone level, has been demonstrated to correlate with the risks of diabetes and cardiovascular disease in euthyroid adults. However, the correlation between thyroid hormone sensitivity and long-term prognosis in euthyroid patients with acute coronary syndrome (ACS) and diabetes after percutaneous coronary intervention (PCI) remains unclear.

Methods

A total of 1,786 euthyroid patients with ACS who successfully underwent PCI at Beijing Anzhen Hospital from August 2021 to April 2022 were included in our study, which was divided into three groups according to tertiles of thyroid hormone sensitivity index. Cox regression, Kaplan–Meier, and receiver operating characteristic analyses were applied to analyze the associations between the FT3/FT4 ratio with ACS and diabetes after PCI.

Results

Our analysis indicated that a lower level of FT3/FT4 ratio in euthyroid patients with acute coronary syndrome (ACS) and diabetes after PCI showed significantly higher incidences of major adverse cardiac and cerebrovascular events (MACCE) when compared with a higher level of FT3/FT4 ratio. After adjusting for other covariates, patients with a lower level of FT3/FT4 ratio were negatively associated with the risk of MACCE than those with a higher level of FT3/FT4 ratio (adjusted OR =1.61, 95% CI 1.05–2.47, P = 0.028). In subgroup analyses, individuals were stratified by age, sex, BMI, ACS type, hypertension, and dyslipidemia, showing that there were no significant interactions between the FT3/FT4 ratio and all subgroups for MACCE. In addition, the FT3/FT4 ratio performed better on ROC analyses for cardiac death prediction [area under the curve (AUC), 0.738].

Conclusion

A reduced level of FT3/FT4 ratio was a potential marker of poor prognosis in euthyroid patients with ACS and diabetes after PCI.
Bi-isotype immunoglobulins enhance antibody-mediated neutrophil activity against Plasmodium falciparum parasites
Authors: Ogwang Rodney , Murugu Lewis , Nkumama Irene N. , Nyamako Lydia , Kai Oscar , Mwai Kennedy , Murungi Linda , Idro Richard , Bejon Philip , Tuju James , Kinyanjui Sam Muchina , Osier Faith H. A.
Published: April 2024

Background

Malaria remains a major global health priority, and monoclonal antibodies (mAbs) are emerging as potential new tools to support efforts to control the disease. Recent data suggest that Fc-dependent mechanisms of immunity are important mediators of protection against the blood stages of the infection, but few studies have investigated this in the context of mAbs. We aimed to isolate mAbs agnostic to cognate antigens that target whole merozoites and simultaneously induce potent neutrophil activity measured by the level of reactive oxygen species (ROS) production using an antibody-dependent respiratory burst (ADRB) assay.

Methods

We used samples from semi-immune adults living in coastal Kenya to isolate mAbs that induce merozoite-specific ADRB activity. We then tested whether modifying the expressed IgG1 isotype to an IgG–IgA Fc region chimera would enhance the level of ADRB activity.

Results

We isolated a panel of nine mAbs with specificity to whole merozoites. mAb J31 induced ADRB activity in a dose-dependent fashion. Compared to IgG1, our modified antibody IgG–IgA bi-isotype induced higher ADRB activity across all concentrations tested. Further, we observed a negative hook effect at high IgG1 mAb concentrations (i.e., >200 µg/mL), but this was reversed by Fc modification. We identified MSP3.5 as the potential cognate target of mAb J31.

Conclusions

We demonstrate an approach to engineer mAbs with enhanced ADRB potency against blood-stage parasites.
The role of dendritic cells in MASH: friends or foes?
Authors: Pinto Antonio T. , Lukacs-Kornek Veronika
Published: April 2024

Dendritic cells (DCs) are major antigen-presenting cells that connect innate and adaptive immunity. Hepatic DCs are less activated and contribute to maintain the tolerogenic environment of the liver under steady state. Several studies indicated DCs in metabolic dysfunction-associated steatohepatitis (MASH), representing a substantial burden on healthcare systems due to its association with liver-related morbidity and mortality. Studies highlighted the potential disease-promoting role of liver DCs in the development of MASH while other experimental systems suggested their protective role. This review discusses this controversy and the current understanding of how DCs affect the pathogenesis of MASH.
Changes in size-dependent Chlorophyll a concentration and group-specific picophytoplankton abundance in short-term nutrient-addition experiments in the Equatorial Eastern Indian Ocean
Authors: Sun Ping , Sun Kai-Ming , Zhou Yuping , Liu Lu , Yuan Chao , Xu Zongjun , Li Yan , Zhang Xuelei , Wang Zongling
Published: April 2024

To clarify the changes in phytoplankton community and influencing factors in short-term nutrient-addition experiments in the Equatorial Eastern Indian Ocean, we conducted three experiments (one in situ-like experiment, one on-deck experiment with deep seawater, and one on-deck experiment with surface seawater). Our findings indicate that when nutrients were added, there was a more significant increase in the chlorophyll a (chl a) concentrations of microphytoplankton (>20 μm) compared to those of nanophytoplankton (2-20 μm) and picophytoplankton (<2 μm). The chl a concentrations for phytoplankton <20 μm only exhibited significant increases in the on-board incubation of surface seawater collected at 1300 hr when grazing stress have likely been weak. In picophytoplankton, occasional increases in the abundances of Synechococcus were found, while the abundances of Prochlorococcus and eukaryotic picophytoplankton (Peuk) did not increase significantly. It results likely from the preference of grazing effect by herbivores and bottle effects. Additionally, the Prochlorococcus from 75 m was more adapted to weak light, thus its abundance sharply decreased when incubated under high light. We suggest that the nutrient effects have greater influence on microphytoplankton, but other factors, such as grazing and light, might contribute more to <20 μm phytoplankton. Furthermore, bottle effects should be considered when conducting incubation experiments.
Case report: Cerebral sinus vein thrombosis in VEXAS syndrome
Authors: Zisapel Michael , Seyman Estelle , Molad Jeremy , Hallevi Hen , Mauda-Havakuk Michal , Jonas-Kimchi Tali , Elkayam Ori , Eviatar Tali
Published: April 2024

VEXAS (vacuoles, E1 enzyme, X-linked, autoinflammatory, and somatic) syndrome is a newly described hemato-inflammatory acquired monogenic entity that presents in adulthood. One of the main features of VEXAS syndrome is a high venous thromboembolism (VTE) burden, with approximately 30–40% experiencing lower extremity deep vein thrombosis and a lower incidence of pulmonary embolism at approximately 10%. To date, VEXAS syndrome has not been associated with rarer forms of VTE such as cerebral sinus vein thrombosis (CSVT) and Budd–Chiari syndrome, which are well-recognized vascular manifestations in Behcet’s disease, another autoinflammatory vasculitic disease. Herein, we describe a case of acute severe extensive and fatal CSVT in a patient with VEXAS syndrome. The event occurred during a period of apparently quiescent inflammatory status, while the patient was receiving tocilizumab and a low dose of glucocorticoids. Despite treatment with anticoagulation, high-dose glucocorticoids, endovascular thrombectomy, and intracranial pressure-lowering agents, the patient suffered severe neurologic damage and ultimately succumbed to the condition 3 weeks after the onset of CSVT. To the best of our knowledge, this is the first reported case of CVST in a patient with VEXAS syndrome.
Bibliometric and visualized analysis of posterior chamber phakic intraocular lens research between 2003 and 2023
Authors: Ning Jiliang , Zhang Qiaosi , Liang Wei , Zhang Rui , Xing Zequn , Jin Lin , Zhang Lijun
Published: April 2024

Introduction

Myopia is causing a major public health concern, with its prevalence increasing globally. This study aimed to discuss posterior chamber phakic intraocular lens (pIOL) research publication trends and hotspots over the past 20 years.

Methods

Bibliometric analysis was performed using the Web Science Core Collection to investigate posterior-chamber pIOL research publication trends. The extracted records were analyzed, and a knowledge map was built using VOSviewer v.1.6.20. The analysis included visualizing the annual publication count, countries/regions distribution, international and institutional collaborations, author productivity, and journal contribution, in addition to identifying knowledge bases and hotspots. Burst keywords were extracted using CiteSpace v.6.1.R.

Results

In total, 791 articles on posterior chamber pIOLs published between 2003 and 2023 were retrieved. China had the highest number of publications, whereas Japanese papers received the most citations. Fudan University had the highest number of publications, with articles from Kitasato University having the highest number of citations. Regarding individual research, Xingtao Zhou has published the most significant number of articles, and Shimizu Kimiya had the highest number of citations. The top productive/influential journal was ‘Journal of Cataract & Refractive Surgery’. The top cited references primarily focused on reporting the clinical outcomes of implantable collamer lens (ICL) for individuals with moderate to high myopia. The keywords primarily formed four clusters: posterior chamber pIOL clinical outcomes for myopic astigmatism correction, posterior chamber pIOL implantation complications, ICL size selection and postoperative vault predictions, and postoperative visual quality following posterior chamber pIOL implantation.

Conclusion

This study presents the first bibliometric analysis of research trends in posterior chamber pIOL over the past two decades. We investigated the current state and emerging trends of global collaboration and research focal points in this field, offering fresh insights and guidance for researchers.
Antibacterial efficacy of novel bismuth-silver nanoparticles synthesis on Staphylococcus aureus and Escherichia coli infection models
Authors: Castro-Valenzuela Beatriz Elena , Franco-Molina Moisés Armides , Zárate-Triviño Diana Ginette , Villarreal-Treviño Licet , Kawas Jorge R. , García-Coronado Paola Leonor , Sobrevilla-Hernández Gustavo , Rodríguez-Padilla Cristina
Published: April 2024

Introduction

The emergence of multi-drug-resistant bacteria is one of the main concerns in the health sector worldwide. The conventional strategies for treatment and prophylaxis against microbial infections include the use of antibiotics. However, these drugs are failing due to the increasing antimicrobial resistance. The unavailability of effective antibiotics highlights the need to discover effective alternatives to combat bacterial infections. One option is the use of metallic nanoparticles, which are toxic to some microorganisms due to their nanometric size.

Methods

In this study we (1) synthesize and characterize bismuth and silver nanoparticles, (2) evaluate the antibacterial activity of NPs against Staphylococcus aureus and Escherichia coli in several infection models (in vivo models: infected wound and sepsis and in vitro model: mastitis), and we (3) determine the cytotoxic effect on several cell lines representative of the skin tissue.

Results and discussion

We obtained bimetallic nanoparticles of bismuth and silver in a stable aqueous solution from a single reaction by chemical synthesis. These nanoparticles show antibacterial activity on S. aureus and E. coli in vitro without cytotoxic effects on fibroblast, endothelial vascular, and mammary epithelium cell lines. In an infected-wound mice model, antibacterial effect was observed, without effect on in vitro mastitis and sepsis models.
The emerging roles of microbiota-derived extracellular vesicles in psychiatric disorders
Authors: Guo Chuang , Bai Yulong , Li Pengfei , He Kuanjun
Published: April 2024

Major depressive disorder, schizophrenia, and bipolar disorder are three major psychiatric disorders that significantly impact the well-being and overall health of patients. Some researches indicate that abnormalities in the gut microbiota can trigger certain psychiatric diseases. Microbiota-derived extracellular vesicles have the ability to transfer bioactive compounds into host cells, altering signaling and biological processes, ultimately influencing the mental health and illness of the host. This review aims to investigate the emerging roles of microbiota-derived extracellular vesicles in these three major psychiatric disorders and discusses their roles as diagnostic biomarkers and therapies for these psychiatric disorders.
Botulinum toxin A injection for post-stroke upper limb spasticity and rehabilitation practices from centers across Asian countries
Authors: Rosales Raymond L. , Chia Nicholas V. C. , Kumthornthip Witsanu , Goh Khean Jin , Mak Choon Soon , Kong Keng He , Ng Yee Sien , Chou Li Wei , Flordelis M. Jeanne , Do Thuy , Maisonobe Pascal , Li Leonard S. W. , Suputtitada Areerat
Published: April 2024

Purpose

Describe real-life practice and outcomes in the management of post-stroke upper limb spasticity with botulinum toxin A (BoNT-A) in Asian settings.

Methods

Subgroup analysis of a prospective, observational study (NCT01020500) of adult patients (≥18 years) with post-stroke upper limb spasticity presenting for routine spasticity management, including treatment with BoNT-A. The primary outcome was goal attainment as assessed using goal-attainment scaling (GAS). Patients baseline clinical characteristics and BoNT-A injection parameters are also described.

Results

Overall, 51 patients from Asia were enrolled. Rates of comorbid cognitive and emotional problems were relatively low. Patients tended to have more severe distal limb spasticity and to prioritize active over passive function goals. Most (94.1%) patients in the subgroup were treated with abobotulinumtoxinA. For these patients, the median total dose was 500 units, and the most frequently injected muscles were the biceps brachii (83.3%), flexor carpi radialis (72.9%), and flexor digitorum profundus (66.7%). Overall, 74.5% achieved their primary goal and the mean GAS T score after one treatment cycle was 56.0 ± 13.0, with a change from baseline of 20.9 ± 14.3 (p < 0.001). The majority (96.1%) of Asian patients were rated as having improved.

Conclusion

In the Asian treatment setting, BoNT-A demonstrated a clinically significant effect on goal attainment for the real-life management of upper limb spasticity following stroke.
Correlation between obstructive sleep apnea and hypoperfusion in patients with acute cerebral infarction
Authors: Zhou Yi , Jin Xiaomei , Liu Xiaorong , Tang Jiafan , Song Liyan , Zhu Yu , Zhai Wanqing , Wang Xianhui
Published: April 2024

Purpose

To explore the relationship between obstructive sleep apnea (OSA) and hypoperfusion during ultra-early acute cerebral infarction.

Patients and methods

Data were retrospectively collected from patients admitted to our hospital with acute cerebral infarction between January 2020 and January 2022, who underwent comprehensive whole-brain computed tomography perfusion imaging and angiography examinations within 6 h of onset. The F-stroke software automatically assessed and obtained relevant data (Tmax). The patients underwent an initial screening for sleep apnea. Based on their Apnea-Hypopnea Index (AHI), patients were categorized into an AHI ≤15 (n = 22) or AHI >15 (n = 25) group. The pairwise difference of the time-to-maximum of the residue function (Tmax) > 6 s volume was compared, and the correlation between AHI, mean pulse oxygen saturation (SpO2), oxygen desaturation index (ODI), percentage of time with oxygen saturation < 90% (T90%), and the Tmax >6 s volume was analyzed.

Results

The Tmax >6 s volume in the AHI > 15 group was significantly larger than that in the AHI ≤ 15 group [109 (62–157) vs. 59 (21–106) mL, p = 0.013]. Spearman’s correlation analysis revealed Tmax >6 s volume was significantly correlated with AHI, mean SpO2, ODI, and T90% in the AHI > 15 group, however, no significant correlations were observed in the AHI ≤ 15 group. Controlling for the site of occlusion and Multiphase CT angiography (mCTA) score, AHI (β = 0.919, p < 0.001), mean SpO2 (β = −0.460, p = 0.031), ODI (β = 0.467, p = 0.032), and T90% (β =0.478, p = 0.026) remained associated with early hypoperfusion in the AHI > 15 group.

Conclusion

In patients with acute cerebral infarction and AHI > 15, AHI, mean SpO2, ODI and T90% were associated with early hypoperfusion. However, no such relationship exists among patients with AHI ≤ 15.
Intraductal magnetic resonance imaging of cholangiocarcinoma - a practical possibility
Authors: Syms Richard R. A. , Wadsworth Christopher A. , Kardoulaki Evdokia , Titapun Attapol , Boonphongsathien Wuttisak , Sa-Ngiamwibool Prakasit , Zhang Shuo , Taylor-Robinson Simon D. , Chamadol Nittaya , Loilome Watcharin
Published: April 2024

Intraductal T2 mapping based on a catheter receiver is proposed as a method of visualizing the extent of intraductal and periductal cholangiocarcinoma (CCA). Compared to external receivers, internal receivers provide locally enhanced signal-to-noise ratios by virtue of their lower field-of-view for body noise, allowing smaller voxels and higher resolution. However, inherent radial sensitivity variation and segmentation for patient safety both distort image brightness. We discuss simulated T2 weighted images and T2 maps, and in vitro images obtained using a thin film catheter receiver of a freshly resected liver specimen containing a polypoid intraductal tumor from a patient with CCA. T2 mapping provides a simple method of compensating non-uniform signal reception patterns of catheter receivers, allowing the visualization of tumor extent without contrast enhancement and potentially quantitative tissue characterization. Potential advantages and disadvantages of in vivo intraductal imaging are considered.
Efficacy and safety of empiric treatment with omeprazole continuous infusion in critically ill children with gastrointestinal bleeding
Authors: Taher Khalid W. , Yaseen Rahaf , Alnan Mayas , Aburas Wejdan , Khalil Hala , Alabdulsalam Moath
Published: April 2024

Introduction

Gastrointestinal bleeding (GI) is a prevalent condition among pediatric patients, with a reported incidence of 6.4%, often severe enough to require admission to the pediatric intensive care unit (PICU). There are multiple therapies utilized in the management of GI bleeding in pediatrics, among which continuous intravenous (IV) infusion of omeprazole is used off-label without standard pediatric dosing recommendations. Reviewing the current literature reveals a lack of studies assessing the efficacy, safety, and appropriate dosing regimen of continuous omeprazole infusion in children with GI bleeding. This study aimed to evaluate the efficacy and safety of continuous IV omeprazole infusion in comparison to other therapeutic modalities in children.

Methods

This study is a single-center, retrospective chart review of children admitted to the PICU at King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. The treatment group included pediatric patients with GI bleeding and receiving omeprazole IV continuous infusion over ≥24 h while the control group included pediatric patients with GI bleeding managed using other therapies. Primary outcomes were the efficacy of omeprazole continuous infusion in stopping GI bleeding, and PICU length of stay (LOS). Secondary outcomes included instances of rebleeding post- therapy discontinuation, transfusion requirements, and the safety of omeprazole continuous infusion.

Results

The study included 81 critically ill pediatric patients, 22 of whom received continuous infusion omeprazole while 59 received other therapies. The results indicated that patients in the control group had a significantly shorter PICU LOS (8 vs. 18.5 days, p < 0.001) and bleeding episode (4 vs. 10.5 days, p < 0.001) than those in the treatment group. However, no significant differences were observed between the two groups regarding secondary outcomes. The treatment group had a significantly lower all-cause mortality rate during hospitalization compared to the control group (16 patients [72.7%] vs. 56 patients [94.9%], respectively, p = 0.005).

Conclusion

Empirical use of omeprazole continuous intravenous infusion in children with GI bleeding was not favorable in terms of shortening PICU LOS and duration of GI bleeding. Our study results provide evidence supporting the safety and tolerability of omeprazole continuous infusion. Additional larger studies are necessary to determine the implication of such results.
Isorhamnetin: what is the in vitro evidence for its antitumor potential and beyond?
Authors: Lei Jiaming , Yang Jianbao , Bao Cuiyu , Lu Feifei , Wu Qing , Wu Zihan , Lv Hong , Zhou Yanhong , Liu Yifei , Zhu Ni , Yu You , Zhang Zhipeng , Hu Meichun , Lin Li
Published: April 2024

Isorhamnetin (ISO) is a phenolic compound belonging to flavonoid family, showcasing important in vitro pharmacological activities such as antitumor, anti-inflammation, and organ protection. ISO is predominantly extracted from Hippophae rhamnoides L. This plant is well-known in China and abroad because of its “medicinal and food homologous” characteristics. As a noteworthy natural drug candidate, ISO has received considerable attention in recent years owing to its low cost, wide availability, high efficacy, low toxicity, and minimal side effects. To comprehensively elucidate the multiple biological functions of ISO, particularly its antitumor activities and other pharmacological potentials, a literature search was conducted using electronic databases including Web of Science, PubMed, Google Scholar, and Scopus. This review primarily focuses on ISO’s ethnopharmacology. By synthesizing the advancements made in existing research, it is found that the general effects of ISO involve a series of in vitro potentials, such as antitumor, protection of cardiovascular and cerebrovascular, anti-inflammation, antioxidant, and more. This review illustrates ISO’s antitumor and other pharmacological potentials, providing a theoretical basis for further research and new drug development of ISO.
Activation of p53 signaling and regression of breast and prostate carcinoma cells by spirooxindole-benzimidazole small molecules
Authors: Barakat Assem , Alshahrani Saeed , Al-Majid Abdullah Mohammed , Alamary Abdullah Saleh , Haukka Matti , Abu-Serie Marwa M. , Dömling Alexander , Domingo Luis R. , Elshaier Yaseen A. M. M.
Published: April 2024

This study discusses the synthesis and use of a new library of spirooxindole-benzimidazole compounds as inhibitors of the signal transducer and activator of p53, a protein involved in regulating cell growth and cancer prevention. The text includes the scientific details of the [3 + 2] cycloaddition (32CA) reaction between azomethine ylide 7a and ethylene 3a within the framework of Molecular Electron Density Theory. The mechanism of the 32CA reaction proceeds through a two-stage one-step process, with emphasis on the highly asynchronous transition state structure. The anti-cancer properties of the synthesized compounds, particularly 6a and 6d, were evaluated. The inhibitory effects of these compounds on the growth of tumor cells (MDA-MB 231 and PC-3) were quantified using IC50 values. This study highlights activation of the p53 pathway by compounds 6a and 6d, leading to upregulation of p53 expression and downregulation of cyclin D and NF-κB in treated cells. Additionally, we explored the binding affinity of spirooxindole analogs, particularly compound 6d, to MDM2, a protein involved in regulation of p53. The binding mode and position of compound 6d were compared with those of a co-crystallized standard ligand, suggesting its potential as a lead compound for further preclinical research.