bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensor based Dance Coherent Action Generation Model using Deep Learning Framework
Authors: Jiang Hanzhen , Yan Yingdong
Published: February 2024

Dance Coherent Action Generation is a popular research task in recent years to generate movements and actions for computer-generated characters in a simulated environment. It is sometimes referred to as "Motion Synthesis". Motion synthesis algorithms are used to generate physically believable, visually compelling, and contextually appropriate movement using motion sensors. The Dance Coherent Action Generation Model (DCAM) is a generative framework for producing aesthetically pleasing movements using deep learning from small amounts of data. By learning an internal representation of motion dynamics, DCAM can synthesize long sequences of movements in which coherent patterns can be created through latent space interpolation. This framework provides a mechanism for varying the amplitude of the generated motion, allowing further realistic thinking and expression. The proposed model obtained 93.79% accuracy, 93.79% precision, 97.75% recall and 92.92% F1 score. DCAM exploits the balance between imitation and creativity by enabling the production of novel outputs from limited input data and can be trained in an unsupervised manner or fine-tuned with sparse supervision. Furthermore, the framework is easily extended to handle multiple layers of abstraction and can be further personalized to a particular type of movement, enabling the generation of highly individualized outputs.
Optimizing Multichannel Path Scheduling in Cognitive Radio Ad Hoc Networks using Differential Evolution
Authors: Dasari Ramesh , N Venkatram
Published: February 2024

One important area of study in cognitive radio ad hoc networks is multi-channel path scheduling. Cognitive radio networks have trouble communicating and using the spectrum effectively because of weather and dispersion. Optimizing multichannel path scheduling enhances network performance and reliability in a cognitive radio ad hoc net. The Optimizing Multichannel Path Scheduling (OMPS) model methodically tackles various scheduling problems. For the OMPS model, this domain is new. It effectively resolves multichannel path scheduling. The computer method used in the study is called Differential Evolution. During optimization, several factors are carefully considered, including Channel Fade Margin, Cross-Correlation and Coherence Time, Spectral Efficiency, Interference Level, Power Consumption, Retransmission Rate, Access Probability, and Propagation Delay. To increase the scheduling efficiency of the DE algorithm, many steps are meticulously planned: initialization, mutation, crossover, fitness evaluation, selection for iteration evolution, and termination. Latency, Packet Delivery Ratio (PDR), Spectrum Utilization, Interference Level, Energy Efficiency, and Established Path Success Rate are all assessed by the OMPS model. These indicators assess the effectiveness and dependability of the network. OMPS performs better in crucial simulations than the existing model. The demonstration demonstrates decreased latency for real-time applications, greater packet delivery ratios (PDRs), improved spectrum efficiency, channel interference, energy efficiency, and connection formation odds, as well as increased throughput that enhances network resource utilization. To do this, a variety of multichannel path scheduling situations are simulated.
Intelligent Navigation System based on Big Data Traffic System
Authors: Zhang Xiu , Kang Jian , Yu Haicun
Published: February 2024

This paper studies the navigation technology of the On-Orbit Servicing Spacecraft (OSS) and proposes an overall solution for the OSS navigation system. The composition of the OSS satellite navigation simulation system and simulation supporting environment are studied. Then a new SSUKF navigation filter is proposed based on the hyper spherical distributed feature sampling point transform algorithm (SSUT) and the non-scanning Kalman filter (UKF). The numerical simulation of the OSS system is studied based on MATLAB/RTW. Finally, the SSUKF algorithm and the conventional UKF algorithm are simulated digitally. The effectiveness and advanced nature of the hybrid Kalman filter applied to spacecraft autonomous navigation are verified.
Optimal Usage of Resources through Quality Aware Scheduling in Containers based Cloud Computing Environment
Authors: S.A. Poojitha , K Ravindranath
Published: February 2024

For cloud computing, the Quality Aware Scheduling of Containers (QASC) model has been proposed for delay-sensitive tasks. Plan your cloud tasks. Typically, time constraints are met while resources are used effectively. It's a really difficult undertaking. In order to distribute containers more effectively, QASC takes into account a number of performance factors. Containers and their make-span logs, as well as input quality metrics like I/O-intensive workload, startup time, hot standby failure rate, and inter-container dependencies, are collected by the QASC model. A metric coefficient that indicates each container's overall rating is calculated by normalizing and averaging these values to determine it. In order to determine how well scheduling performed, the model also includes a quality coefficient that calculates this metric-coefficient threshold. It's also critical for QASC to be able to determine the remaining energy in each container, which represents its request capacity. In order to optimize cloud resources, energy use is also taken into account by the model. From the cloud-sim simulation, an experimental dataset including 50 containers and 1,200 internet protocol-capable users was employed. For the make-span ratio, round-trip time, and energy consumption analysis, this produced 20,000 data points. The RLSched, DSTS, and ADATSA models were contrasted with the QASC model. The outcomes showed that QASC performed better than these models in a number of crucial areas. Tasks may be managed better with the higher average make-span ratio and lower volatility. Its superior job scheduling and resource use were further demonstrated by its shorter round-trip durations and lower energy usage across loads. The QASC model is an extremely complex scheduling method for container-based systems and a significant advancement in cloud computing research. Its approaches and methods enable for more intelligent energy use as well as high-quality services while also improving system performance, particularly for tasks that are delay-sensitive.
Quality Enhancement with Frame-wise DLCNN using High Efficiency Video Coding in 5G Networks
Authors: Dommeti Vijaya Saradhi , Dharani M. , Shasidhar K. , Rami Reddy Y Dasaratha , Moorthy T. Venkatakrishna
Published: February 2024

In the present situation, applications related to multimedia are discovered to be comfortable with the use of video. The number of end consumers who use video continues to rise every day. People are presently searching for videos with better quality among the ones that are currently available there. This results in the launch and dissemination of HD (high definition) videos. Ultra high-definition (UHD) videos are becoming more and more popular as a result of this advancement and need. However, as video communication keeps expanding, there is an upsurge in network traffic because of the limited bandwidth, especially among smart cities. Different advancement codecs have been suggested to deal with the data stream to overcome this hazardous circumstance. However, the fact that modern UHD videos have huge amounts of data makes the available codecs even more complicated. UHD videos can be processed with the latest improvement codec, H.265/High-efficiency video coding (HEVC). Nevertheless, it is impacted by increased power consumption and intricate calculations. Limitations in the codec's functionality confine its use to specific applications, preventing its application in wireless, mobile, or portable settings. Hence, this research concentrates on implementing frame-level quality enhancement through a deep learning network known as FQE-Net. The deep learning convolutional neural network (DLCNN) is specifically crafted to manage films with resolutions up to 16K. Its primary objectives include reducing complexity, minimizing artifacts, enhancing the efficiency of the HEVC codec, and compacting energy consumption. To achieve superior efficiency, it is imperative to replace the DWT transforms within the HEVC codec with a DLCNN model. Additionally, incorporating the Content Block Search Algorithm for Motion Estimation and Compensation, alongside filtering techniques like Sample Adaptive Filter and Deblocking Filter, becomes essential. The simulation results showed that the suggested FQE-Net performed better than the conventional techniques.
Determination of hand functionality in patients with rheumatoid arthritis based on the results of a physical therapy programme
Authors: Nogas A. , Grygus I.
Published: February 2024

At present, there is a significant increase in the prevalence of rheumatoid arthritis, which significantly affects the patient's quality of life and leads to a permanent loss of their ability to work, which is an important medical and social problem in Ukraine. The purpose of the paper is to determine the functional capabilities of the hand in patients with rheumatoid arthritis according to the results of the physical therapy programme. Materials and methods. The study examined 188 patients with rheumatoid arthritis, whose average age was 44.9±7.6 years. All patients were randomly assigned to the control (n=92) and intervention (n=96) groups. Patients in the control group underwent rehabilitation in accordance with the recommendations of the regulatory document of the Ministry of Health of Ukraine. For the patients of the main group, a rehabilitation intervention technology was scientifically substantiated and developed, including physical therapy means with a personalised approach to each patient, taking into account the factors affecting the level of functional impairment and quality of life. The physical therapy programme included the following elements: therapeutic exercises, therapeutic massage, physiotherapy, hydrotherapy, orthotics, kinesiotaping of the upper extremities, mechanotherapy and psychological support. The Sollerman hand function test was used to determine the functional capabilities of the hand. Results. Increased mobility in the joints of the upper extremities and muscle strength 3 months after both standard treatment and physical therapy contributed to the improvement of manipulative function and activity of the hand in everyday life, as evidenced by the Sollerman test. However, significantly better changes occurred in patients of the main group compared to the control group. Thus, in patients of the main group, after 3 months, the average Sollerman test scores increased to 65.1±5.3 points, in the control group this figure was significantly lower than 61.8±3.6 points (±S) (р<0,05). Accordingly, the average Sollerman test score in patients of the main group increased from 65.1±5.3 points to 70.2±6.3 points, which is significantly higher than in patients of the control group – from 61.8±3.6 points to 65.1±3.8 points (±S) (р<0,05). Conclusion. After a 6-month course of physical therapy, patients in the main group showed better results in the Sollerman test than in the control group, which indicates the effectiveness of the proposed technology of rehabilitation measures. Consequently, the functional capacity of the hands significantly increased in the patients of the main group, which improved self-care, performance, daily activity and quality of life.
case of a patient with type 1 diabetes mellitus and anorexia nervosa with changes to the central nervous system
Authors: Bajaka Armand
Published: February 2024

Co-occurrence of anorexia nervosa (AN) and diabetes mellitus type 1 (T1DM) is an uncommon disease combination in clinical practice.  Treatment of T1DM may affect outcomes in AN patients due to self-modification of insulin dosage. In the presented case 14-year-old patient diagnosed with both T1DM and AN has been modifying her insulin-dependent treatment to prevent gaining weight. Undercontrolled glucose levels resulted in early-stage diabetic vascular complications in the central nervous system found in MRI.  Due to higher T1DM morbidity of eating disorders patients, adjustments and special control of insulin treatment and blood glucose levels as well as HbA1c should be maintained in the prevention of diabetic complications, especially in individuals with lower treatment compliance. As AN may lead to structural changes and a decrease in cognitive functions,  increased supervision of AN patients with T1DM can lower the risk of cumulative negative effects on the brain.
The safety of tear trough deformity treatment: an overview
Authors: Ramadan Francesca
Published: February 2024

The use of minimally invasive cosmetic interventions is constantly growing, and tear trough deformity treatment using dermal fillers is one of the non-surgical procedures that is in increasing demand. However, there is also growing public concern about the safety of this treatment option, and patients may be particularly anxious due to the proximity of the eyeball to the injection site. In this article, Francesca Ramadan outlines the risks associated with this procedure and the elements required for careful patient consideration and selection.
Resolving problems at work
Authors: Bernstein Adam
Published: February 2024

Adam Bernstein explains why mediation is a useful rescoure for those looking to preserve professional relationships and deal with work-related issues amicably
Static and Dynamic Analysis of Coupled Vibration of Suspension Bridge Structure Under Earthquake Action
Authors: XiaoJian Xu , GuangTao Xin , Jian Zhao , Yu Chen
Published: February 2024

This article investigates the coupled vibration problem of suspension bridge structures under earthquake action, and conducts static and dynamic analysis using computer algorithms. In terms of static analysis, complex structures are divided into many small elements, and the stress and deformation of each element are calculated through finite element analysis, thereby obtaining the static characteristics of the entire structure. In terms of dynamic analysis, the seismic response analysis method based on time history considers seismic action as a time-varying external excitation, and calculates and simulates the dynamic response of the suspension bridge structure. In order to achieve efficient and accurate calculations, this article also adopts optimization algorithms and numerical calculation methods. Through a comprehensive study of statics and dynamics analysis, the key characteristics and regularities of coupled vibration of suspension bridge structures under earthquake action have been obtained. These research results not only provide important theoretical guidance and technical support for related engineering practices, but also provide useful references and inspirations for dynamic analysis and optimization design of similar structures. Meanwhile, using computer algorithms and simulation software for static and dynamic analysis can significantly reduce analysis costs and time, improve analysis accuracy and efficiency, and have broad application prospects.
Analysis on Mechanical Properties of FRP Constrained Concrete Core Reinforcement Under Cyclic Load
Authors: Zhenlong Tong , Zenglin Wu , Dongyu Yang , Zhongya Han
Published: February 2024

This article aims to analyse the mechanical properties of FRP confined concrete core rods under cyclic loading. By observing the behaviour characteristics of FRP confined concrete core rods under different cyclic loads, the influence of FRP confinement on concrete cyclic loading is analysed. Based on theoretical analysis, this article establishes a corresponding mechanical model and analyses the response mechanism of FRP constraints to concrete cyclic loads through simulation calculations. Through experimental research and theoretical analysis, it is possible to fully understand the performance of new composite structural materials. The research results of this article indicate that the confinement of FRP bars can significantly improve the compressive strength of concrete specimens. At a constraint ratio of 20%, the compressive strength of the specimen increased by 30% compared to the unconstrained ratio; At 40%, the compressive strength increased by 50%, therefore, FRP confinement can significantly improve the ductility of concrete and reduce fatigue damage under cyclic loading. Through this research method, important evidence can be provided for the application of concrete in practical engineering.
Analysis on Shear Resistance of Joint and Structural Mechanical Response of Assembled Composite Beam Joints
Authors: Zhifang Ma , Shihao Zhang , Xiaoguang Guo
Published: February 2024

This article systematically studies the shear characteristics and structural mechanical response characteristics of composite beam joints. The results indicate that the shear stiffness of the connector is between 1000 N/mm and 2500 N/mm, with high stiffness performance. Meanwhile, as the stiffness increases, the frequency of the structure shows a significant increasing trend, indicating that high stiffness helps to improve the stability of the structure. In the trend chart of shear characteristics and performance of connectors over time, the shear characteristics of connectors significantly increased between 2018 and 2023, with an average shear strength increasing from 1500 N to 2100 N, an increase of 40%. By studying the static and dynamic response characteristics, as well as the bearing capacity, stability, and fatigue performance of composite beams. The results indicate that the response characteristics of the new connector are significantly better than those of traditional connectors. Based on the research results, this article proposes strategies and suggestions for optimizing connector design and improving overall structural performance, providing important theoretical support and practical guidance for practical engineering applications. This study not only enriches the theoretical system of composite beams, but also has important significance in promoting technological progress in related fields.
Extracorporeal Shock Wave Treatment for Calcifying Tendinitis of the Shoulder: A Case Report and Literature Review
Authors:   Peking University Health Science Center, China-Japan Friendship School of Clinical Medicine, Beijing, China,, Song Dehui , Li Chengxin ,   Department of Orthopedic, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China,, Sun Wei ,   [3] Department of Orthopedics, Shockwave Center, China-Japan Friendship Hospital, Beijing, China, [4] Department of Orthopedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Zhou Yu ,   Peking University Health Science Center, China-Japan Friendship School of Clinical Medicine, Beijing, China,, Gao Fuqiang ,   Department of Orthopedics, Shockwave Center, China-Japan Friendship Hospital, Beijing, China,, Zirong Li ,   Peking University Health Science Center, China-Japan Friendship School of Clinical Medicine, Beijing, China,
Published: February 2024

Background: Calcifying tendinitis of the shoulder (CTS), also known as rotator cuff calcific tendinitis, is a self-limiting shoulder disorder primarily characterized by inflammation surrounding hydroxyapatite crystal deposits in the tendons of the rotator cuff. Given the specific characteristics of the shoulder joint and the uncertainty regarding the efficacy of various treatments for CTS, no standardized treatment protocol has been established. However, numerous studies have demonstrated that extracorporeal shock wave therapy (ESWT) is effective in alleviating pain and improving joint function in patients with calcifying tendinitis of the shoulder. The therapeutic process works using high-energy shock waves to break down deposits of calcification, reduce local inflammation, and promote tissue healing. The purpose of this article is to present a case of symptomatic calcifying tendinopathy involving the rotator cuff and further demonstrate that ESWT has good efficacy in the treatment of musculoskeletal diseases. Results: In this case, a 39-year-old woman with a 6-month history of shoulder pain and limited range of motion (ROM) was diagnosed with calcifying tendinitis of the shoulder. A single session of shock wave therapy resulted in the complete resolution of calcific deposits and the patient’s symptoms, leading to a return to her normal ROM and improved quality of life. Conclusion: ESWT has been shown to be a good alternative to surgery for the treatment of calcifying tendinitis of the shoulder (CTS). It can significantly alleviate pain, improve shoulder function, and reduce the average size of calcium deposits. It might be the first choice for treating shoulder tendinopathy due to its effectiveness and safety. Keywords: Calcifying tendinitis, Shoulder pain, Extracorporeal shock wave treatment, Case report
Chinese expert consensus on clinical drug prevention and treatment of osteonecrosis of the femoral head（2023)
Authors:   [1] Centre for Osteonecrosis and Joint-Preserving & Reconstruction, Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China. [2] Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Sun Wei , Gao Fuqiang ,   Centre for Osteonecrosis and Joint-Preserving & Reconstruction, Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China., Li Zirong ,   Centre for Osteonecrosis and Joint-Preserving & Reconstruction, Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China., Yang Xu ,   Centre for Osteonecrosis and Joint-Preserving & Reconstruction, Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China., Lu Jike ,   Centre for Osteonecrosis and Joint-Preserving & Reconstruction, Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China.
Published: February 2024

The in-depth understanding of osteonecrosis of the femoral head (ONFH), has lead more and more patients to seek for medical treatment in the early stage of the disease. Surgical treatment of femoral head necrosis alone is no longer sufficient for the current patients’ demand. The rational and effective use of drugs to strengthen the prevention and early treatment of femoral head necrosis delaying the progression of the disease, is becoming more and more important. This article combines the latest expert consensus and evidence-based medical research on the principles of ONFH diagnosis and treatment according to Chinese and Western medicine and is organized by Chinese experts from the Association Related to Osseous Circulation and the Chinese Microcirculation Society (CSM-ARCO). This consensus was formulated with focus on drug types, characteristics, and safety. Rationality and consideration of basic principles of drug use will provide safe, reasonable, standardized, and effective drug use in medical institutions at all levels. This consensus is only an expert guideline based on literature and clinical experience, not a requirement for mandatory implementation. The clinical practice can be tailored to the actual local conditions to develop appropriate prevention and treatment measures for patients. Keywords: Osteonecrosis of the femoral head; Expert consensus; Drug prevention and treatment
Imaging of Isolated Exosomes by Correlative Microscopy
Authors: Demir Şeyda , Erdal Esra , Bagriyanik Hüsnü Alper
Published: February 2024

Correlative microscopy is a sophisticated imaging technique that combines optical and electron microscopes, with the most common approach being the integration of light microscopy and electron microscopy, known as correlative light and electron microscopy (CLEM). While CLEM provides a comprehensive view of biological samples, it presents a significant challenge in sample preparation due to the distinct processes involved in each technique. Striking a balance between these methods is crucial. Despite numerous approaches, achieving seamless imaging with CLEM remains a complex task. Exosomes, nanovesicles ranging from 30 to 150 nm in size, are enclosed by a lipid bilayer and released by various cell types. Visualizing exosomes poses difficulties due to their small size and minimal electric charge. However, imaging exosomes at high resolution offers a direct method to understand their morphology and functions. In this study, we evaluated exosome imaging with CLEM using a combination of confocal, transmission electron microscope, and scanning electron microscope (SEM). In addition, we conducted a comparative analysis of these two techniques, evaluating their suitability and efficiency in imaging nanoscale structures. In this study, we found that confocal-SEM correlation is more applicable for imaging exosomes. Moreover, we observed that exosomes were found in clusters in confocal-SEM correlation.
Farmers’ perceptions of bovine brucellosis in Benin
Authors: Djibril Adeyemi Sharafa Dine , Bothon Fifa Théomaine Diane , Boko Kadoeto Cyrille , Koutinhouin Bénoit Gbetondjingninougbo , Farougou Souaibou
Published: February 2024

Background and Aim: Cattle are the main source of meat in Benin. To improve the attitudes and practices of cattle breeders in relation to bovine brucellosis, a study has been carried out in Benin according to different agroecological zones. This study aimed to assess farmers’ knowledge and practices concerning bovine brucellosis to generate essential information for control programs and public health interventions.

Materials and Methods: The study was conducted from February to May 2022, during which 608 farmers were interviewed using a structured questionnaire that provided information on socioeconomic characteristics, knowledge, and practices related to bovine brucellosis. Analysis of variance , Poisson regression, and the proportion comparison test were used to compare these characteristics in the different agroecological zones. At the end of the surveys, three distinct and homogeneous groups of perceptions (hierarchical classification of Multiple Correspondence Analysis components of R software) of bovine brucellosis were identified (these groups only consider farmers who declared knowledge of the disease). Groups were formed by applying the multiple correspondence analysis function of the FactoMineR library in R software, followed by a hierarchical ascending classification using the hierarchical clustering on principal component function of the same software (Agrocampus Rennes, France).

Results: Only 38% of respondents were aware of brucellosis. Knowledge of brucellosis was not related to sex or education level but was higher among farmers in agroecological Zones 1 and 4. Ethnic Dendi herders (62.16%) had better knowledge of the disease than those from other sociolinguistic groups (Somba: 50%, Fulani: 40.91%, Baribas: 26.97%, and others: 8.82%). Reduced milk production (98.29%), presence of hygroma (87.18%), and abortion (56.84%) are the main signs reported by herders familiar with the disease. All three groups had good knowledge of the disease and its zoonotic nature. Groups 1 (96% of breeders) and 2 (2.14%) were aware of the risk factors (contact with affected animals, the consumption of raw milk, the handling of runts, and reproductive rejection). In the case of Brucella, they prefer to treat animals rather than sell them and use both traditional and modern medicines. Group 3 (1.71%) did not know the risk factors and preferred to sell animals in the event of illness.

Conclusion: Pastoralists need to be made aware of the mode of transmission of bovine brucellosis, its clinical manifestations, its impact on animal health, and the zoonotic nature of the disease (impact on public health) so that bovine brucellosis can be rapidly detected in herds.

Keywords: Benin, brucellosis risk factors, public health, knowledge.
Effect of probiotics and acidifiers on feed intake, egg mass, production performance, and egg yolk chemical composition in late-laying quails
Authors: Lokapirnasari Widya Paramita , Al-Arif Mohammad Anam , Hidayatik Nanik , Safiranisa Aldhia , Arumdani Dynda Febriana , Zahirah Amadea Inas , Yulianto Andreas Berny , Lamid Mirni , Marbun Tabita Dameria , Lisnanti Ertika Fitri , Baihaqi Zein Ahmad , Khairullah Aswin Rafif , Kurniawan Shendy Canadya , Pelawi Erlycasna Beru Sembiring , Hasib Abdullah
Published: February 2024

Background and Aim: Probiotics can be used as an alternative to antibiotic growth promoters because antibiotics are prohibited worldwide. This study investigated the potential combination of probiotics and acidifiers to improve feed intake, productive performance, egg mass, and egg yolk chemical composition of late-laying quail for the health of humans who consume quail products.

Materials and Methods: One hundred laying quails were divided into 4 × 5 treatments, with each group consisting of five replications. The adaptation period was 2 weeks, and the treatment was continued for 4 weeks. Probiotics and acidifiers were added to drinking water and incorporated into the diet. Feed and water were provided ad libitum. Treatment duration (1 week, 2 weeks, 3 weeks, and 4 weeks) and additional feed treatment (control, probiotic 2% + 0.5% acidifier, probiotic 2% + 1% acidifier, probiotic 4% + 0.5% acidifier, and probiotic 4% + 1% acidifier, respectively).

Results: Significant differences (p < 0.05) were observed in feed intake, quail day production, feed efficiency, egg mass in laying quails, and the chemical composition of egg yolk with probiotics and acidifiers in late-laying quails.

Conclusion: The combination of probiotics and acidifiers can improve feed intake, production performance, egg mass, and egg yolk chemical composition in late-laying quails.

Keywords: acidifiers, feed additive, health, performance, probiotic.
Valproic Acid Decreases the Nuclear Localization of MDT-28, the Nematode Orthologue of MED28
Authors: Kostrouchová Markéta , Kostrouchová V. , Yilma P. , Benda A. , Mandys V. , Kostrouchová Marta
Published: February 2024

Mediator is a multiprotein complex that connects regulation mediated by transcription factors with RNA polymerase II transcriptional machinery and integrates signals from the cell regulatory cascades with gene expression. One of the Mediator subunits, Mediator complex subunit 28 (MED28), has a dual nuclear and cytoplasmic localization and function. In the nucleus, MED28 functions as part of Mediator and in the cytoplasm, it interacts with cytoskeletal proteins and is part of the regulatory cascades including that of Grb2. MED28 thus has the potential to bring cytoplasmic regulatory interactions towards the centre of gene expression regulation. In this study, we identified MDT-28, the nematode orthologue of MED28, as a likely target of lysine acetylation using bioinformatic prediction of posttranslational modifications. Lysine acetylation was experimentally confirmed using anti-acetyl lysine antibody on immunoprecipitated GFP::MDT-28 expressed in synchronized C. elegans. Valproic acid (VPA), a known inhibitor of lysine deacetylases, enhanced the lysine acetylation of GFP::MDT-28. At the subcellular level, VPA decreased the nuclear localization of GFP::MDT-28 detected by fluorescencelifetime imaging microscopy (FLIM). This indicates that the nuclear pool of MDT-28 is regulated by a mechanism sensitive to VPA and provides an indirect support for a variable relative proportion of MED28 orthologues with other Mediator subunits.
Extracellular DNA as a Prognostic and Therapeutic Target in Mouse Colitis under DNase I Treatment
Authors: Bábíčková J. , Čonka J. , Janovičová L. , Boriš M. , Konečná B. , Gardlík Roman
Published: February 2024

The aim of this study was to investigate the potential of extracellular DNA as a prognostic and/or therapeutic target in inflammatory bowel disease. Fifty male C57BL/6J mice were used in the experiment. Acute colitis was induced by intake of 2% dextran sulphate sodium (DSS) for seven days followed by three days of water intake. DNase I was injected intravenously on days 3 and 7. Plasmatic levels of extracellular DNA (ecDNA) were measured on days 6 and 10. Weight loss, stool consistency and liquid intake were monitored throughout the experiment. Colon length and weight, myeloperoxidase activity and tumour necrosis factor α (TNF-α) levels were measured at sacrifice. DSS-treated mice displayed severe colitis, as shown by disease activity parameters. Both groups with colitis (DNase treated and untreated) had significantly poorer weight loss, colon length and stool consistency compared with control groups on water. No differences between the DNasetreated and untreated DSS groups were recorded. Myeloperoxidase activity and levels of TNF-α in colonic tissue were notably greater in both groups with colitis compared to controls. In addition, both biochemical markers were improved in the DNasetreated group with colitis compared to the untreated group. Although the disease activity was proved by several independent parameters in both groups with colitis, levels of ecDNA did not show any difference between the groups throughout or at the end of experiment. The role of ecDNA in experimental colitis has not been confirmed. However, DNase I injection resulted in some improvement, and thus should be studied in more detail.
Pyrogallol Induces Apoptosis in Human Platelets
Authors: Bruges G. , Venturini W. , Crespo G. , López Zambrano Mercedes
Published: February 2024

Pyrogallol is a polyphenol that generates the superoxide anion. In this study, we investigated the influence of pyrogallol on human platelets. Our data showed that exposure of platelets to pyrogallol induced numerous manifestations of apoptosis including depolarization of mitochondrial inner membrane and release of cytochrome c from the mitochondria. Pyrogallol also induced downstream extra-mitochondrial apoptotic responses, including activation of caspase-3 and phosphatidylserine exposure on the outer leaflet of the plasma membrane. Addition of glutathione significantly rescued cells from pyrogallol- induced apoptosis, as evidenced by a decrease of all markers of apoptosis. Thus, pyrogallol appears to produce depletion of intracellular glutathione content in platelets, the main non-protein antioxidant in the cells. Furthermore, inhibition of γ-glutamyl transpeptidase, an enzyme that plays the main role in the cellular supply of glutathione, reverted the glutathione (GSH) protection over platelet apoptosis. Our results indicate that pyrogallol induces apoptosis by suppressing the natural anti-oxidation in human platelets.
Buccal Respiratory Chain Complexes I and IV Quantities in Huntington’s Disease Patients
Authors: Dušek P. , Rodinová M. , Lišková I. , Klempíř J. , Zeman J. , Roth J. , Hansíková Hana
Published: February 2024

Alterations in mitochondrial parameters are an important hallmark of Huntington’s disease (HD). The ubiquitous expression of mutant huntingtin raises the prospect that mitochondrial disturbances can also be detected and monitored through buccal epithelial cells. In a group of 34 patients with Huntington’s disease and a group of 22 age-related healthy volunteers, respiratory complex I and IV protein quantities in buccal epithelial cells were measured using the dipstick immunocapture assay. The protein quantity of respiratory complex I correlates with age (r = 0.427, P = 0.026, FWE-P = 0.156) in the patient group, but not in the group of healthy subjects. Our non-invasive approach allows us to obtain valuable information for the studies of mitochondrial biochemical parameters in patients with neurodegenerative diseases and could also be useful in epidemiological studies.
Vibration acceleration enhances proliferation, migration, and maturation ofC2C12cells and promotes regeneration of muscle injury in male rats
Authors: Sato Seira , Hanai Tatsuhiro , Kanamoto Takashi , Kawano Fuminori , Hikida Minami , Yokoi Hiroyuki , Take Yasuhiro , Magome Takuya , Ebina Kosuke , Mae Tatsuo , Tanaka Hiroyuki , Nakata Ken
Published: February 2024

Vibration acceleration (VA) using a whole‐body vibration device is beneficial for skeletal muscles. However, its effect at the cellular level remains unclear. We aimed to investigate the effects of VA on muscles in vitro and in vivo using the C2C12 mouse myoblast cell line and cardiotoxin‐induced injury in male rat soleus muscles. Cell proliferation was evaluated using the WST/CCK‐8 assay and proportion of Ki‐67 positive cells. Cell migration was assessed using wound‐healing assay. Cell differentiation was examined by the maturation index in immunostained cultured myotubes and real‐time polymerase chain reaction. Regeneration of soleus muscle in rats was assessed by recruitment of satellite cells, cross‐sectional area of regenerated muscle fibers, number of centrally nucleated fibers, and conversion of regenerated muscle from fast‐ to slow‐twitch. VA at 30 Hz with low amplitude for 10 min promoted C2C12 cell proliferation, migration, and myotube maturation, without promoting expression of genes related to differentiation. VA significantly increased Pax7‐stained satellite cells and centrally nucleated fibers in injured soleus muscles on Day 7 and promoted conversion of fast‐ to slow‐twitch muscle fibers with an increase in the mean cross‐sectional area of regenerated muscle fibers on Day 14. VA enhanced the proliferation, migration, and maturation of C2C12 myoblasts and regeneration of injured rat muscles.
Pheochromocytoma-induced myocardial infarction: A case report
Authors: Tang Haixia , Liu Jichun , Hu Bangsheng , Yang Yuwen , Xie Xiangrong , Wei Youquan
Published: February 2024

The pheochromocytoma is an uncommon endocrine neoplasm that originates from chromaffin cells and causes significant cardiovascular effects through the intermittent or sustained release of catecholamines. In this report, we present a rare case of myocardial infarction (MI) induced by pheochromocytoma. A 53-year-old female presented to the emergency department with a history of intermittent palpitations, back pain, and sweating for over 10 years, which had worsened over the past 2 days. The patient’s cardiac enzymes and troponin levels were significantly elevated, and the electrocardiogram (ECG) showed ST-segment elevation, leading to an initial diagnosis of acute myocardial infarction. Echocardiography revealed apical ballooning, indicative of stress cardiomyopathy. Emergency coronary angiography revealed no significant stenosis, and the patient’s blood pressure was fluctuating. Computerized tomography (CT) scan of the adrenal gland revealed a bilateral adrenal mass, with the left adrenal mass being larger in size after contrast-enhanced CT scan. The patient’s left adrenal gland was successfully removed through laparoscopic adrenalectomy, and histopathology results confirmed the presence of adrenal pheochromocytoma. Follow-up for 3 months after discharge showed the patient had no symptoms and good prognosis. The abnormal findings on echocardiography and ECG resolved. Prompt diagnosis and management of pheochromocytoma are crucial for a favorable prognosis.
Comparing Emergency Remote Learning with Traditional Learning in Primary Education: Primary School Student Perspectives
Authors: Lampropoulos Georgios , Admiraal Wilfried
Published: February 2024

This study aims to explore primary school students’ perspectives of emergency remote teaching and learning during the COVID-19 pandemic and their preferences when comparing it with face-to-face learning. Data from 114 Greek primary students showed that students did not find online learning particularly intriguing, enjoyable, and interactive and felt social isolation due to the lack of communication and interactions. Most students preferred traditional learning to online learning as they found it more enjoyable and interactive, and made it easier to learn, focus on, comprehend, and perform better. They greatly valued the class atmosphere and the ability to actively participate and freely communicate, and stated that it was easier to devote time to studying, complete their assignments, and get answers to their question when learning in person. Students believed that despite its disadvantages, online learning might be more broadly used in the future. Finally, no significant differences were found regarding students’ gender, age, and class level.
Random forest and artificial neural network-based tsunami forests classification using data fusion of Sentinel-2 and Airbus Vision-1 satellites: A case study of Garhi Chandan, Pakistan
Authors: Mateen Shabnam , Nuthammachot Narissara , Techato Kuaanan
Published: February 2024

This article proposes random forest algorithm (RFA), multi-layer perception (MLP) artificial neural network (ANN), and support vector machine (SVM) method for classifying the fused data of Sentinel-2, Landsat-8, and Airbus Vision-1 satellites for the years 2016 and 2023. The first variant of fusion is performed for Sentinel-2 and Landsat-8 data to sharpen it to 10 m spatial resolution, while in the second case, Sentinel-2 and Airbus Vision-1 data are fused together to achieve a spatial resolution of 3.48 m. MLP-ANN, SVM, and RFA methods are applied to the sharpened dataset for the years 2023 and 2016 having spatial resolutions of 3.48 and 10 m, respectively, and a detailed comparative analysis is performed. Google earth engine is utilized for ground data validation of the classified samples. An enhanced convergence time of 100 iterations was achieved using MLP-ANN for the classification of the dataset at 3.48 m spatial resolution, while the same method took 300 iterations with the dataset at 10 m spatial resolution to achieve a minimum limit Kappa hat score of 0.85. With 10 m spatial resolution, the MLP-ANN achieved an overall accuracy of 96.6% and a Kappa hat score of 0.94, while at 3.48 m spatial resolution, the aforementioned scores are enhanced to 98.5% and 0.97, respectively. Similarly, with 10 m spatial resolution, the RFA achieved an overall accuracy of 92.6% and a Kappa hat score of 0.88, while at 3.48 m spatial resolution, the abovementioned scores are enhanced to 96.5 and 0.95% respectively. In view of the forgoing, the MLP-ANN showed better performance as compared to the RFA method.
Crystal structure of (6-chloropyridin-3-yl)methyl 2-(6-methoxynaphthalen-2-yl)propanoate, C20H18ClNO3
Authors: Bai Jing , Huo Rui-Li , Li Dong-Fei , Yang Ying
Published: February 2024

C20H18ClNO3, monoclinic, P21 (no. 4), a = 11.6954(2) Å, b = 5.7274(10) Å, c = 26.5974(5) Å, β = 102.177(10)°, V = 1741.52(5) Å3, Z = 4, Rgt
(F) = 0.0282, wRref
(F
2) = 0.0728, T = 170(2) K.
The diameter of the Birkhoff polytope
Authors: Bouthat Ludovick , Mashreghi Javad , Morneau-Guérin Frédéric
Published: February 2024

The geometry of the compact convex set of alln×nn\times ndoubly stochastic matrices, a structure frequently referred to as the Birkhoff polytope, has been an active subject of research as of late. Geometric characteristics such as the Chebyshev center and the Chebyshev radius with respect to the operator norms fromℓnp{\ell }_{n}^{p}toℓnp{\ell }_{n}^{p}and the Schattenpp-norms, both for the range1≤p≤∞1\le p\le \infty, have only recently been studied in depth. In this article, we continue in this vein by determining the diameter of the Birkhoff polytope with respect to the metrics induced by the aforementioned matrix norms.
Protocol for a Single-Center Randomized Controlled Trial of Percutaneous Coronary Intervention Via Distal Transradial Access Versus Transradial Access
Authors: Liu Minghao , Wang Huanhuan , Gao Lijian , Chen Jue , Cheng Cui , Song Ying , Zhang Hao , Yu Wei , Lang Xinyue , Zhao Honghui , Zhai Jinwei , Tong Yana , Liu Yan , Duan Fujian , Li Hui , Song Yiying , Ye Shaodong , Liu Xiaoning , Wang Yong
Published: February 2024

Background: Although transradial access (TRA) has become the main vascular access for coronary intervention, its high radial artery occlusion rate limits its application in some patients. Studies have shown that compared with TRA, distal transradial access (dTRA) with the snuffbox area or the Hegu acupoint area as the puncture point significantly decreases the incidence of radial artery occlusion. However, no randomized controlled study has confirmed the safety and efficacy of coronary artery intervention via dTRA in China.

Methods and analyses: This single-center, prospective, randomized controlled, superiority open-label study will enroll 428 consecutive patients with coronary heart disease undergoing percutaneous coronary intervention as the study population. After preoperative evaluation, the participants will be randomly divided into a study group (dTRA) and control group (TRA) in a 1:1 ratio. The primary endpoint (radial artery occlusion at 24 hours after operation) and secondary endpoint events will be evaluated and recorded.

Study registration: This study has been registered in the Chinese Clinical Trial Registry (registration number: ChiCTR2300073902).
Evaluation of Real Time PCR Detection of the 47 kDa Gene for Reliable Diagnosis of Pediatric Scrub Typhus
Authors: Chandy Sara , Peddi Sudheer Kumar , Gurumoorthy M. , Ramachandran Hemalatha , Ramya M. S. , Balasubramanian S. , Manoharan Anand
Published: February 2024

Objective:

Diagnosis of scrub typhus is difficult, because of its non-specific clinical features and a lack of reliable serological assays. This study assessed real time PCR detection of the 47 kDa gene along with routine IgM ELISA for confirmatory diagnosis of scrub typhus.

Methods:

This observational study was conducted between March 2021 and February 2023. Real time PCR detection of the 47 kDa gene was used as a diagnostic assay. Confirmed scrub typhus cases were defined by IgM ELISA and/or real time PCR positivity.

Results:

A total of 105 children (0–18 years of age) were enrolled, 30 (28.57%) of whom were confirmed to have scrub typhus, according to IgM ELISA and/or real time PCR positivity. The clinical symptoms of scrub typhus positive children included fever, diarrhea, abdominal pain, breathing difficulties, lymphadenopathy, rash, headache, edema, seizure, and poor intake. Good statistical correlations were observed among real time scrub positivity, high grade fever of 8.2 days’ duration, the presence of eschar and thrombocytopenia. Non-specific respiratory symptoms; cough, and cold were more severe in patients with scrub typhus. The real time PCR test showed good specificity in discriminating scrub typhus from enteric fever, a common pediatric disease.

Conclusion:

Detection of the 47 kDa gene with real time PCR has good sensitivity and specificity. The number of days between fever onset and sampling, as well as the administration of antibiotics before sampling, influence the performance of real-time PCR.
Effects of Furnish-related Factors on Tension and Relaxation of Wet Webs
Authors:   VTT Technical Research Centre of Finland, P.O. Box 1603, FI-40101 Jyväskylä, Finland, Retulainen Elias , Salminen Kristian ,   VTT Technical Research Centre of Finland, P.O. Box 1603, FI-40101 Jyväskylä, Finland
Published: February 2024

A smooth web transfer in a paper machine requires sufﬁcient tension. It is well known that excessive tension leads to web breaks. However, too low a tension can also be fatal. Particularly with wet web, the maintenance of the tension is challenging due to the fast relaxation of the tension. Some factors that affect the tension and relaxation of wet web were studied in this paper. The initial tension and the tension after constant relaxation time, called residual tension, was found to depend on factors such as the applied strain, straining rate, dry solids content, ﬁbre and ﬁnes properties, substances in the white water, and the dry strength chemicals. The residual tension was reduced by increased straining rate, addition of TMP ﬁltrate, and addition of cationic starch. The tension and residual tension seemed to be dependent on both the properties of the ﬁbre fraction, such as in- and out-of plane stiffness, and on the factors affecting the stress transfer conditions at the inter-ﬁbre contact areas.