bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Study Comparing Effects of Microplastic Derived from Bottle Caps Collected in Two Cities on Triticum aestivum (Wheat)
Authors: Pflugmacher Stephan , Tallinen Saila , Mitrovic Simon M. , Penttinen Olli-Pekka , Kim Young-Jun , Kim Sanghun , Esterhuizen Maranda
Published: July 2021

As plastic has become an integral component of daily life, microplastic has become a ubiquitous, unavoidable constituent of nearly all ecosystems. Besides monitoring the amount and distribution of microplastic in the environment, it is necessary to understand the possible direct effects, especially toxicity and how it is affected by environmental factors where it is discarded. The present study investigated how microplastic derived from high-density polyethylene bottle caps collected in two climatically different cities, i.e., Singapore (tropical rainforest climate) and Lahti, Finland (continental climate), affected the essential agricultural grain crop, Triticum aestivum (L.). Wheat seedlings were exposed to microplastic derived from these collected bottle caps, as well as new and artificially aged caps, for seven days. Morphological parameters, such as root and shoot length and oxidative stress development, were measured. Exposure to microplastic derived from the caps resulted in reduced seedling root and shoot lengths compared to the controls, as well as enhanced lipid peroxidation and catalase activity. With all parameters tested, microplastic derived from Lahti bottle caps exhibited more severe effects than Singapore, which was similar to that elicited by new microplastic. The Singapore microplastic had possibly leached its toxic substances before collection due to accelerated degradation promoted by the prevailing warmer climate conditions.
Seismic Performance of RC Beam–Column Joints Designed According to Older and Modern Codes: An Attempt to Reduce Conventional Reinforcement Using Steel Fiber Reinforced Concrete
Authors: Tsonos Alexander-Dimitrios , Kalogeropoulos George , Iakovidis Pantelis , Bezas Marios-Zois , Koumtzis Michail
Published: July 2021

An analytical and experimental investigation was conducted herein to examine the cyclic load behavior of beam–column joint subassemblages, typical of both the modern reinforced concrete (RC) structures and of the pre-1960s–1970s existing ones. Seven exterior RC beam–column joint subassemblages were constructed and subjected to earthquake-type loading. Three specimens were designed according to the requirements of the Eurocode (EC) for ductility class medium (DCM), while the other three specimens possessed poor seismic details, conforming to past building codes. The hysteresis behavior of the subassemblages was evaluated. An analytical model was used to calculate the ultimate shear capacity of the beam–column joint area, while also predicting accurately the failure mode of the specimens. It was clearly demonstrated experimentally and analytically that it is possible for excessive seismic damage of the beam–column joint region to occur when designing according to the current European building codes. In addition, the proposed analytical model was found to be very satisfactory in accurately predicting seismic behavior and in preventing the premature brittle shear failure of the joints. The seventh subassemblage, constructed with steel fiber RC and significantly less transverse reinforcement than that required according to the EC, exhibited satisfactory ductile seismic performance, demonstrating the effectiveness of the proposed design solution.
Free Stream Behavior of Hydrogen Released from a Fluidic Oscillating Nozzle
Authors: Fink Anja , Nett Oliver , Schmidt Simon , Krüger Oliver , Ebert Thomas , Trottner Alexander , Jander Bojan
Published: July 2021

The H2 internal combustion engine (ICE) is a key technology for complete decarbonization of the transport sector. To match or exceed the power density of conventional combustion engines, H2 direct injection (DI) is essential. Therefore, new injector concepts that meet the requirements of a H2 operation have to be developed. The macroscopic free stream behavior of H2 released from an innovative fluidic oscillating nozzle is investigated and compared with that of a conventional multi-hole nozzle. This work consists of H2 flow measurements and injection tests in a constant volume chamber using the Schlieren method and is accompanied by a LES simulation. The results show that an oscillating H2 free stream has a higher penetration velocity than the individual jets of a multi-hole nozzle. This behavior can be used to inject H2 far into the combustion chamber in the vertical direction while the piston is still near bottom dead center. As soon as the oscillation of the H2 free stream starts, the spray angle increases and therefore H2 is also distributed in the horizontal direction. In this phase of the injection process, spray angles comparable to those of a multi-hole nozzle are achieved. This behavior has a positive effect on H2 homogenization, which is desirable for the combustion process.
Predicting the Properties of Industrially Produced Oat Flours by the Characteristics of Native Oat Grains or Non-Heat-Treated Groats
Authors: Jokinen Iina , Pihlava Juha-Matti , Puganen Anna , Sontag-Strohm Tuula , Linderborg Kaisa M. , Holopainen-Mantila Ulla , Hietaniemi Veli , Nordlund Emilia
Published: July 2021

The aim of this study was to determine whether the properties of the native oat grain or non-heat-treated groats (laboratory-scale dehulling) can be used to predict the quality of the industrially produced oat flour produced from heat-treated groats. Quality properties such as the color, hectoliter weight, thousand seed weight and hull content of Finnish native grains (n = 30) were determined. Furthermore, the relationship between the properties of the native grains and the chemical composition of the raw oat materials before and after the milling process were studied. A significant relationship (p < 0.01) was observed between the thousand seed weight of the native oat groats and the chemical composition of the industrially produced oat flour. Furthermore, the protein content of the native grains measured by NIT correlated with the chemical composition of the oat flours. These results suggest that the properties of oat flour produced on an industrial scale, including heat treatment, could be predicted based on the properties of native oat grains.
The Pro-Gly or Hyp-Gly Containing Peptides from Absorbates of Fish Skin Collagen Hydrolysates Inhibit Platelet Aggregation and Target P2Y12 Receptor by Molecular Docking
Authors: Tian Qi , Li Shi-Ming , Li Bo
Published: July 2021

Previous studies found that the collagen hydrolysates of fish skin have antiplatelet activity, but this component remained unknown. In this study, eleven peptides were isolated and identified in the absorbates of Alcalase-hydrolysates and Protamex®-hydrolysates of skin collagen of H. Molitrix by reverse-phase C18 column and HPLC-MS/MS. Nine of them contained a Pro-Gly (PG) or Hyp-Gly (OG) sequence and significantly inhibited ADP-induced platelet aggregation in vitro, which suggested that the PG(OG) sequence is the core sequence of collagen peptides with antiplatelet activity. Among them, OGSA has the strongest inhibiting activities against ADP-induced platelet aggregation in vitro (IC50 = 0.63 mM), and OGSA inhibited the thrombus formation in rats at a dose of 200 μM/kg.bw with no risk of bleeding. The molecular docking results implied that the OG-containing peptides might target the P2Y12 receptor and form hydrogen bonds with the key sites Cys97, Ser101, and Lys179. As the sequence PG(OG) is abundant in the collagen amino acid sequence of H. Molitrix, the collagen hydrolysates of H. Molitrix might have great potential for being developed as dietary supplements to prevent cardiovascular diseases in the future.
A Predictive Strategy Based on Volatile Profile and Chemometric Analysis for Traceability and Authenticity of Sugarcane Honey on the Global Market
Authors: Silva Pedro , Freitas Jorge , Nunes Fernando M. , Câmara José S.
Published: July 2021

Sugarcane honey (SCH) is a syrup produced on Madeira Island and recognized by its unique aroma, a complex attribute of quality with an important influence on the final consumer’s acceptance of the product, and determined by a complex mixture of a large number of volatile organic compounds (VOCs) generated during its traditional making process and storage. Therefore, the purpose of this study was to establish the volatile profile of genuine SCH produced by a regional certified producer for seven years and compare it with syrups from non-certified regional producers and with producers from different geographical regions (Spain, Egypt, Brazil and Australia), as a powerful strategy to define the volatomic fingerprint of SCH. Different volatile profiles were recognized for all samples, with 166 VOCs being identified belonging to different chemical classes, including furans, ketones, carboxylic acids, aldehydes and alcohols. Chemometric analysis allowed (i) the differentiation between all syrups, being more pronounced between SCH and other syrups; and (ii) the identification of 32 VOCs as potential markers for the traceability and authenticity of SCH on the global market.
Natural Bioactive Compounds from Food Waste: Toxicity and Safety Concerns
Authors: Vilas-Boas Ana A. , Pintado Manuela , Oliveira Ana L. S.
Published: July 2021

Although synthetic bioactive compounds are approved in many countries for food applications, they are becoming less and less welcome by consumers. Therefore, there has been an increasing interest in replacing these synthetic compounds by natural bioactive compounds. These natural compounds can be used as food additives to maintain the food quality, food safety and appeal, and as food supplements or nutraceuticals to correct nutritional deficiencies, maintain a suitable intake of nutrients, or to support physiological functions, respectively. Recent studies reveal that numerous food wastes, particularly fruit and vegetables byproducts, are a good source of bioactive compounds that can be extracted and reintroduced into the food chain as natural food additives or in food matrices for obtaining nutraceuticals and functional foods. This review addresses general questions concerning the use of fruit and vegetables byproducts as new sources of natural bioactive compounds that are being addressed to foods as natural additives and supplements. Those bioactive compounds must follow the legal requirements and evaluations to assess the risks for human health and their toxicity must be considered before being launched into the market. To overcome the potential health risk while increasing the biological activity, stability and biodistribution of the supplements’ technological alternatives have been studied such as encapsulation of bioactive compounds into micro or nanoparticles or nanoemulsions. This will allow enhancing the stability and release along the gastrointestinal tract in a controlled manner into the specific tissues. This review summarizes the valorization path that a bioactive compound recovered from an agro-food waste can face from the moment their potentialities are exhibited until it reaches the final consumer and the safety and toxicity challenges, they may overcome.
In Vitro Screening Potential Antibacterial Properties of the Greek Oregano Honey against Clinical Isolates of Helicobacter pylori
Authors: Voidarou Chrysoula (Chrysa) , Rozos Georgios , Alexopoulos Athanasios , Plessas Stavros , Mantzourani Ioanna , Stavropoulou Elisavet , Tzora Athina , Bezirtzoglou Eugenia
Published: July 2021

Oregano honey is an exceedingly rare and distinct product, not commercially available, produced by bees bred in oregano fields of alpine altitudes at the mountainous area of Epirus, Greece. In ethnic popular medicine, this product is used as a therapeutic in various gastric diseases. To test this hypothesis, 14 strains of Helicobacter pylori (H. pylori), 6 isolated from gastric ulcers and 8 from cases of clinical gastritis, were employed in the present study. The above bacterial strains were exposed to various concentrations (75% v/v, 50% v/v, 25% v/v, 12.5% v/v, and 6% v/v) of 50 oregano honey samples by using the agar well method and the inhibition zones observed around each well were recorded. Although the inhibitory zones of the H. pylori isolated from the gastric ulcers were wide enough (0–34 mm), those strains, in general, appeared more resistant than the other eight (0–58 mm). The same result was observed when the same strains were tested against six antibiotics used in clinical practice. Extracts of oregano honey were prepared by extraction with four different organic solvents. N-hexane and chloroform extracts had the most potent antibacterial action. Finally, pure oregano honey and diethyl ether extracts of honey showed significant inhibitory activity against urease secreted by the pathogen. These results strongly indicate the susceptibility of H. pylori strains to the oregano honey by more than one mode of action. Consequently, this variety of honey seems to have potential therapeutic properties against gastric ulcers and gastritis, thus explaining the preference of the locals towards this traditional remedy.
1H NMR Study of the In Vitro Digestion of Highly Oxidized Soybean Oil and the Effect of the Presence of Ovalbumin
Authors: Martin-Rubio Ana S. , Sopelana Patricia , Ibargoitia María L. , Guillén María D.
Published: July 2021

Oxidized lipids containing a wide variety of potentially toxic compounds can be ingested through diet. However, their transformations during digestion are little known, despite this knowledge being essential in understanding their impact on human health. Considering this, the in vitro digestion process of highly oxidized soybean oil, containing compounds bearing hydroperoxy, aldehyde, epoxy, keto- and hydroxy groups, among others, is studied by 1H nuclear magnetic resonance. Lipolysis extent, oxidation occurrence and the fate of oxidation products both present in the undigested oil and formed during digestion are analyzed. Furthermore, the effect during digestion of two different ovalbumin proportions on all the aforementioned issues is also addressed. It is proved that polyunsaturated group bioaccessibility is affected by both a decrease in lipolysis and oxidation occurrence during digestion. While hydroperoxide level declines throughout this process, epoxy-compounds, keto-dienes, hydroxy-compounds, furan-derivatives and n-alkanals persist to a great extent or even increase. Conversely, α,β-unsaturated aldehydes, especially the very reactive and toxic oxygenated ones, diminish, although part of them remains in the digestates. While a low ovalbumin proportion hardly affects oil evolution during digestion, at a high level it diminishes oxidation and reduces the concentration of potentially bioaccessible toxic oxidation compounds.
Online Suicide Identification in the Framework of Rhetorical Structure Theory (RST)
Authors: Liu Xingyun , Liu Xiaoqian
Published: July 2021

Background: Suicide is a serious social problem. Substantial efforts have been made to prevent suicide for many decades. The internet has become an important arena for suicide prevention and intervention. However, to the best of our knowledge, only one study has analyzed suicidal comments online from the perspective of rhetorical structure with incomplete rhetorical relations. We aimed to examine the rhetorical differences between Chinese social media users who died by suicide and those without suicidal ideation. Methods: The posts of 15 users who died by suicide and 15 not suffering from suicide ideation were annotated by five postgraduates with expertise in analyzing suicidal posts based on rhetorical structure theory (RST). Group differences were compared via a chi-square test. Results: Results showed that users who died by suicide posted significantly more posts and used more rhetorical relations. Moreover, the two groups displayed significant differences in 17 out of 23 rhetorical relations. Limitations: Because this study is largely exploratory and tentative, caution should be taken in generalizing our findings. Conclusions: Our results expand the methods of RST to the online suicidal identification field. There are implications for population-based suicide prevention by combining rhetorical structures with context analysis.
Quality of Life, Depression, and Anxiety in Survivors of Critical Illness from a Greek ICU. A Prospective Observational Study
Authors: Vrettou Charikleia S. , Mantziou Vassiliki , Ilias Ioannis , Vassiliou Alice G. , Orfanos Stylianos E. , Kotanidou Anastasia , Dimopoulou Ioanna
Published: July 2021

The physical and psychological consequences of critical illness may affect intensive care unit survivors for up to five years, and hence, health-related quality of life has emerged as an important measure of outcome in this population. We aimed at investigating the quality of life, the presence of anxiety and depression symptoms, and the correlations of clinical and psychological parameters with the quality of life scores in survivors of critical illness one year after discharge from intensive care. Widely used scales that have been validated for the Greek population were used. One thousand two hundred and seventy (1270) patients were assessed for eligibility. Inclusion criteria were age between 18 and 68 years and the presence of critical illness requiring endotracheal intubation and mechanical ventilation for more than three days. One hundred and four (104) patients were included in the final analysis; forty-nine age and sex-matched healthy volunteers were included for comparison. One year following intensive care unit discharge, survivors of critical illness had impaired quality of life scores, as measured with the WHOQOL-Bref, compared to healthy subjects (p < 0.001 for the physical and psychological domains, and p = 0.006 for the domain of social relationships). In addition, we found that quality of life scores were significantly lower in patients with comorbidities (p < 0.01), and correlated with the duration of ICU stay (r = −0.19, p = 0.04) and with the presence of symptoms suggestive of depression (r = −0.66, p < 0.0001) and post-traumatic stress disorder (r = −0.61, p < 0.0001). Approximately one-third of our patients scored high in the CES-D scale for depression, while the majority of them scored high in the STAI scale for anxiety symptoms.
The Effect of Polarized Training on the Athletic Performance of Male and Female Cross-Country Skiers during the General Preparation Period
Authors: Kim Tae Ho , Han Joung Kyue , Lee Ji Young , Choi Yong Chul
Published: July 2021

This study aimed to analyze the effect of 12 weeks of polarized training on body composition, cardiorespiratory function, and upper-body power of male and female cross-country skiers during the general preparation period. A total of 16 national cross-country skiers (8 male and 8 female; 8 national cross-country skiers and 8 national biathlon athletes) participated. Polarization training was conducted for 12 weeks from May to July in 2019 during the general preparation period for cross-country skiers. The low-weight, high-repetition method was used for strength training. The effect of the polarized training on body composition, maximum oxygen intake (VO2max), respiratory exchange rate, all-out time, and ski ergometer exercise time was assessed. There was no change in weight, BMI, and muscle mass in male and female cross-country skiers following the 12 weeks of polarized training (p > 0.05). Male body fat percentage (pre 18.1%, post 12.7%) and female body fat percentage (pre 29.1%, post 21.4%) showed a significant decrease (p < 0.05). After training, VO2max increased by 7.72% in male athletes (pre 71.05 mL/kg/min, post 77.0 mL/kg/min) and 6.32% in female athletes (pre 60.26 mL/kg/min, post 64.33 mL/kg/min). Treadmill exercise time increased by 5.39% for male athletes (pre 1038 s, post 1064 s) and 2.23% for female athletes (pre 855 s, post 874 s). However, there was no significant difference between male and female athletes (p > 0.05). The 50% recovery time from the maximum heart rate to the target heart rate decreased by 64.52% in males (pre 168.8 s, post 102.6 s) and 6.48% in females (pre 135 s, post 129.6 s). Significant differences were found only in male athletes (p < 0.05). The double-pole 500 m exercise duration for the ski ergometer significantly decreased after the training for both sexes (p < 0.05). In this study, the 12 weeks of polarized training improved the body composition and athletic performance of all cross-country skiers. Interestingly, in this study, we confirmed that polarized training had a better effect on cardiorespiratory function in male cross-country skiers than in female cross-country skiers. Conversely, we found that the outcomes of the ski ergometer exercise factors were more effective in female athletes than in male athletes. Therefore, we insist that when applying a polarized training program to athletes, it should be planned in detail by sex, exercise amount, intensity, and type of training.
The Digital Divide: A Retrospective Survey of Digital Rectal Examinations during the Workup of Rectal Cancers
Authors: Farooq Omar , Farooq Ameer , Ghosh Sunita , Qadri Raza , Steed Tanner , Quinton Mitch , Usmani Nawaid
Published: July 2021

Background: Digital rectal examination (DRE) is considered an important part of the physical examination. However, it is unclear how many patients have a DRE performed at the primary care level in the work-up of rectal cancer, and if the absence of a DRE causes a delay to consultation with a specialist. Methods: A retrospective patient questionnaire was sent to 1000 consecutive patients with stage II or stage III rectal cancer. The questionnaire asked patients to recall if they had a DRE performed by their general practitioner (GP) when they first presented with symptoms or a positive FIT test. Demographic data, staging data, and time to consultation with a specialist were also collected. Results: A thousand surveys were mailed out, and a total of 262 patients responded. Of the respondents, 46.2% did not recall undergoing a digital rectal examination by their primary care provider. Women were less likely to undergo a DRE than men (28.6% vs. 44.3%, p = 0.019). While there was a trend towards longer times to specialist consultation in patients who did not undergo a DRE (27.0 vs. 12.2 weeks), this was not statistically significant (p = 0.121). Conclusion: A significant proportion of patients who are FIT positive or have symptomatic rectal bleeding do not recall having a DRE by their primary care provider. Barriers may include lack of comfort with performing DRE or lack of time. Clearer guidelines and more support for GP’s may increase uptake of DRE.
Relationship between Training Load Management and Immunoglobulin A to Avoid Immunosuppression after Soccer Training and Competition: A Theoretical Framework Based on COVID-19 for Athletes’ Healthcare
Authors: Rico-González Markel , Pino-Ortega José , Clemente Filipe , Bustamante-Hernández Naia
Published: July 2021

Immunoglobulin A (IgA), which is the main effector against upper respiratory tract viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been related to training load management. The aim of this systematic review was to establish the relationship between training load and salivary IgA based on current evidence in order to avoid immunosuppression after exercise and players´ vulnerability to virus contagion. A systematic review of relevant articles was carried out using two electronic databases (PubMed and Web of Science) until 19 May 2021. From a total of 127 studies initially found, 23 were included in the qualitative synthesis. These studies were clustered depending on stress level. The salivary IgA was analysed considering soccer-specific treadmill exercise and repeated sprint drills (n = 5), matches (n = 7), and during certain periods during the season or pre-season (n = 11). Repeated sprint ability tests and treadmill exercises are suitable exercises for the first steps on return to play periods yet still maintain social distance. A rest or moderate training sessions (technical/tactical) are suggested after official matches to ensure 16–18 h to recover IgA levels, while periods with multiple matches per week with limited recovery time should be avoided. Weekly training load should assume a small increment (<10%) to ensure IgA immune responses, especially, during the post coronavirus disease 2019 (COVID-19) season.
Influence of Berry Ripening Stages over Phenolics and Volatile Compounds in Aged Aglianico Wine
Authors: Genovese Alessandro , Basile Boris , Lamorte Simona Antonella , Lisanti Maria Tiziana , Corrado Giandomenico , Lecce Lucia , Strollo Daniela , Moio Luigi , Gambuti Angelita
Published: July 2021

The harvest time of grapes is a major determinant of berry composition and of the wine quality, and it is usually established through empirical testing of main biochemical parameters of the berry. In this work, we studied how the ripening stage of Aglianico grapes modulates key secondary metabolites of wines, phenolics and volatile compounds. Specifically, we analyzed and compared four berry ripening stages corresponding to total soluble solids of 18, 20, 22, and 25 °Brix and related aged wines. Wine color intensity, anthocyanins level and total trans-resveratrol (free + glycosidic form) increased with grape maturity degree. Wines obtained from late-harvested grapes significantly differed from the others for a higher content of aliphatic alcohols, esters, acetates, α-terpineol and benzyl alcohol. The content of glycosidic terpene compounds, such as nerol, geraniol and α-terpineol, was higher in wines obtained with grapes harvested at 25 °Brix compared to the earlier harvests. Our work indicated that the maturity of the grape is a determining factor in phenolic and volatile compounds of red Aglianico wines. Moreover, extending grape ripening to a sugar concentration higher than 22 °Brix improves the biochemical profile of aged wine in terms of aroma compounds and of phytochemicals with known health-related benefits.
Attachment, Emotion Dysregulation, and Physical IPV in Predominantly Hispanic, Young Adult Couples
Authors: Pollard Deanna L. , Cantos Arthur L.
Published: July 2021

Insecure attachment has been found to be a risk factor for perpetrating physical intimate partner violence (IPV). However, this association is likely exacerbated by additional factors, such as conflicting insecure attachment in one’s partner and difficulties with overall emotion regulation and impulse control. The present study aimed to examine the associations between insecure attachment and physical IPV perpetration in male and female partners, as well as to examine whether these associations are exacerbated by involvement with a partner with opposing attachment needs and overall emotion dysregulation and impulsivity. Additionally, this study examined whether partners’ emotion dysregulation interacted to predict IPV. Two hundred eight heterosexual couples primarily recruited from a Hispanic-serving university completed questionnaires on attachment, emotion dysregulation, and one’s own and one’s partner’s perpetration. Results revealed that attachment anxiety, impulsivity, and an interaction effect between attachment avoidance and partner’s attachment anxiety were associated with self-reported, but not partner-reported, male perpetration. For females, attachment anxiety was associated with female IPV (self-reported and partner-reported), and impulsivity was associated with self-reported female IPV. Overall, results underscore how relationships between known risk factors and IPV perpetration may differ depending on if IPV perpetration is measured using self-reported or partner-reported data. Additional results and implications are discussed.
Physical Fitness among Community-Dwelling Older Women with and without Risk of Falling—The Taipei Study, Taiwan
Authors: Song Chen-Yi , Tsauo Jau-Yih , Fang Pei-Hsin , Fang I-Yao , Chang Shao-Hsi
Published: July 2021

The purposes of this study were to compare the differences in physical fitness between community-dwelling older women fallers and non-fallers, with and without a risk of falling, and to investigate the relation between physical fitness and falling risk factors. This study was a secondary data analysis from a community- and exercise-based fall-prevention program. Baseline assessments pertaining to body weight and height, self-reported chronic diseases, the 12-item fall risk questionnaire (FRQ), senior fitness test, single-leg stand test, and handgrip strength test were extracted. Participants (n = 264) were classified into fallers and non-fallers, and sub-classified according to the risk of falling (FRQ ≥4 and <4). While controlling for the effect of age, body mass index (BMI), and multimorbidity, one-way analysis of covariance indicated that older women with a risk of falling showed poorer performances of the 8-foot up-and-go, 2-min step and 30-s chair stand compared with those without a risk of falling, regardless of the history of falls. Additionally, weaker grip strength was found in non-fallers with falling risk. Some significant, but low-to-moderate, correlations were found between physical fitness tests and fall risk factors in the FRQ, particularly in gait/balance problem and leg muscle weakness. Proactive efforts are encouraged to screen and manage deterioration in the identified physical fitness.
An “Animated Spatial Time Machine” in Co-Creation: Reconstructing History Using Gamification Integrated into 3D City Modelling, 4D Web and Transmedia Storytelling
Authors: Matthys Mario , De Cock Laure , Vermaut John , Van de Weghe Nico , De Maeyer Philippe
Published: July 2021

More and more digital 3D city models might evolve into spatiotemporal instruments with time as the 4th dimension. For digitizing the current situation, 3D scanning and photography are suitable tools. The spatial future could be integrated using 3D drawings by public space designers and architects. The digital spatial reconstruction of lost historical environments is more complex, expensive and rarely done. Three-dimensional co-creative digital drawing with citizens’ collaboration could be a solution. In 2016, the City of Ghent (Belgium) launched the “3D city game Ghent” project with time as one of the topics, focusing on the reconstruction of disappeared environments. Ghent inhabitants modelled in open-source 3D software and added animated 3D gamification and Transmedia Storytelling, resulting in a 4D web environment and VR/AR/XR applications. This study analyses this low-cost interdisciplinary 3D co-creative process and offers a framework to enable other cities and municipalities to realise a parallel virtual universe (an animated digital twin bringing the past to life). The result of this co-creation is the start of an “Animated Spatial Time Machine” (AniSTMa), a term that was, to the best of our knowledge, never used before. This research ultimately introduces a conceptual 4D space–time diagram with a relation between the current physical situation and a growing number of 3D animated models over time.
Role of Maximum Entropy and Citizen Science to Study Habitat Suitability of Jacobin Cuckoo in Different Climate Change Scenarios
Authors: Singh Priyanka , Saran Sameer , Kocaman Sultan
Published: July 2021

Recent advancements in spatial modelling and mapping methods have opened up new horizons for monitoring the migration of bird species, which have been altered due to the climate change. The rise of citizen science has also aided the spatiotemporal data collection with associated attributes. The biodiversity data from citizen observatories can be employed in machine learning algorithms for predicting suitable environmental conditions for species’ survival and their future migration behaviours. In this study, different environmental variables effective in birds’ migrations were analysed, and their habitat suitability was assessed for future understanding of their responses in different climate change scenarios. The Jacobin cuckoo (Clamator jacobinus) was selected as the subject species, since their arrival to India has been traditionally considered as a sign for the start of the Indian monsoon season. For suitability predictions in current and future scenarios, maximum entropy (Maxent) modelling was carried out with environmental variables and species occurrences observed in India and Africa. For modelling, the correlation test was performed on the environmental variables (bioclimatic, precipitation, minimum temperature, maximum temperature, precipitation, wind and elevation). The results showed that precipitation-related variables played a significant role in suitability, and through reclassified habitat suitability maps, it was observed that the suitable areas of India and Africa might decrease in future climatic scenarios (SSPs 2.6, 4.5, 7.0 and 8.5) of 2030 and 2050. In addition, the suitability and unsuitability areas were calculated (in km2) to observe the subtle changes in the ecosystem. Such climate change studies can support biodiversity research and improve the agricultural economy.
Cytokine/Chemokine Release Patterns and Transcriptomic Profiles of LPS/IFNγ-Activated Human Macrophages Differentiated with Heat-Killed Mycobacterium obuense, M-CSF, or GM-CSF
Authors: Bazzi Samer , El-Darzi Emale , McDowell Tina , Modjtahedi Helmout , Mudan Satvinder , Achkar Marcel , Akle Charles , Kadara Humam , Bahr Georges M.
Published: July 2021

Macrophages (Mφs) are instrumental regulators of the immune response whereby they acquire diverse functional phenotypes following their exposure to microenvironmental cues that govern their differentiation from monocytes and their activation. The complexity and diversity of the mycobacterial cell wall have empowered mycobacteria with potent immunomodulatory capacities. A heat-killed (HK) whole-cell preparation of Mycobacterium obuense (M. obuense) has shown promise as an adjunctive immunotherapeutic agent for the treatment of cancer. Moreover, HK M. obuense has been shown to trigger the differentiation of human monocytes into a monocyte-derived macrophage (MDM) type named Mob-MDM. However, the transcriptomic profile and functional properties of Mob-MDMs remain undefined during an activation state. Here, we characterized cytokine/chemokine release patterns and transcriptomic profiles of lipopolysaccharide (LPS)/interferon γ (IFNγ)-activated human MDMs that were differentiated with HK M. obuense (Mob-MDM(LPS/IFNγ)), macrophage colony-stimulating factor M-MDM(LPS/IFNγ)), or granulocyte/macrophage colony-stimulating factor (GM-MDM(LPS/IFNγ)). Mob-MDM(LPS/IFNγ) demonstrated a unique cytokine/chemokine release pattern (interleukin (IL)-10low, IL-12/23p40low, IL-23p19/p40low, chemokine (C-x-C) motif ligand (CXCL)9low) that was distinct from those of M-MDM(LPS/IFNγ) and GM-MDM(LPS/IFNγ). Furthermore, M-MDM(LPS/IFNγ) maintained IL-10 production at significantly higher levels compared to GM-MDM(LPS/IFNγ) and Mob-MDM(LPS/IFNγ) despite being activated with M1-Mφ-activating stimuli. Comparative RNA sequencing analysis pointed to a distinct transcriptome profile for Mob-MDM(LPS/IFNγ) relative to both M-MDM(LPS/IFNγ) and GM-MDM(LPS/IFNγ) that comprised 417 transcripts. Functional gene-set enrichment analysis revealed significant overrepresentation of signaling pathways and biological processes that were uniquely related to Mob-MDM(LPS/IFNγ). Our findings lay a foundation for the potential integration of HK M. obuense in specific cell-based immunotherapeutic modalities such as adoptive transfer of Mφs (Mob-MDM(LPS/IFNγ)) for cancer treatment.
Metabolite Profiling and Transcriptome Analysis Provide Insight into Seed Coat Color in Brassica juncea
Authors: Shen Shulin , Tang Yunshan , Zhang Chao , Yin Nengwen , Mao Yuanyi , Sun Fujun , Chen Si , Hu Ran , Liu Xueqin , Shang Guoxia , Liu Liezhao , Lu Kun , Li Jiana , Qu Cunmin
Published: July 2021

The allotetraploid species Brassica juncea (mustard) is grown worldwide as oilseed and vegetable crops; the yellow seed-color trait is particularly important for oilseed crops. Here, to examine the factors affecting seed coat color, we performed a metabolic and transcriptomic analysis of yellow- and dark-seeded B. juncea seeds. In this study, we identified 236 compounds, including 31 phenolic acids, 47 flavonoids, 17 glucosinolates, 38 lipids, 69 other hydroxycinnamic acid compounds, and 34 novel unknown compounds. Of these, 36 compounds (especially epicatechin and its derivatives) accumulated significantly different levels during the development of yellow- and dark-seeded B. juncea. In addition, the transcript levels of BjuDFR, BjuANS,BjuBAN, BjuTT8, and BjuTT19 were closely associated with changes to epicatechin and its derivatives during seed development, implicating this pathway in the seed coat color determinant in B. juncea. Furthermore, we found numerous variations of sequences in the TT8A genes that may be associated with the stability of seed coat color in B. rapa, B. napus, and B. juncea, which might have undergone functional differentiation during polyploidization in the Brassica species. The results provide valuable information for understanding the accumulation of metabolites in the seed coat color of B. juncea and lay a foundation for exploring the underlying mechanism.
Ginsenoside Re Protects against Serotonergic Behaviors Evoked by 2,5-Dimethoxy-4-iodo-amphetamine in Mice via Inhibition of PKCδ-Mediated Mitochondrial Dysfunction
Authors: Shin Eun-Joo , Jeong Ji Hoon , Nguyen Bao-Trong , Sharma Naveen , Nah Seung-Yeol , Chung Yoon Hee , Lee Yi , Byun Jae Kyung , Nabeshima Toshitaka , Ko Sung Kwon , Kim Hyoung-Chun
Published: July 2021

It has been recognized that serotonin 2A receptor (5-HT2A) agonist 2,5-dimethoxy-4-iodo-amphetamine (DOI) impairs serotonergic homeostasis. However, the mechanism of DOI-induced serotonergic behaviors remains to be explored. Moreover, little is known about therapeutic interventions against serotonin syndrome, although evidence suggests that ginseng might possess modulating effects on the serotonin system. As ginsenoside Re (GRe) is well-known as a novel antioxidant in the nervous system, we investigated whether GRe modulates 5-HT2A receptor agonist DOI-induced serotonin impairments. We proposed that protein kinase Cδ (PKCδ) mediates serotonergic impairments. Treatment with GRe or 5-HT2A receptor antagonist MDL11939 significantly attenuated DOI-induced serotonergic behaviors (i.e., overall serotonergic syndrome behaviors, head twitch response, hyperthermia) by inhibiting mitochondrial translocation of PKCδ, reducing mitochondrial glutathione peroxidase activity, mitochondrial dysfunction, and mitochondrial oxidative stress in wild-type mice. These attenuations were in line with those observed upon PKCδ inhibition (i.e., pharmacologic inhibitor rottlerin or PKCδ knockout mice). Furthermore, GRe was not further implicated in attenuation mediated by PKCδ knockout in mice. Our results suggest that PKCδ is a therapeutic target for GRe against serotonergic behaviors induced by DOI.
MCPIP1/Regnase-1 Expression in Keratinocytes of Patients with Hidradenitis Suppurativa: Preliminary Results
Authors: Krajewski Piotr K. , Szukała Weronika , Lichawska-Cieślar Agata , Matusiak Łukasz , Jura Jolanta , Szepietowski Jacek C.
Published: July 2021

The pathogenesis of hidradenitis suppurativa (HS) is yet to be fully understood. However, inflammation is a key element in the development of skin lesions. The aim of this study was to evaluate the expression of monocyte chemotactic protein-1-induced protein-1 (MCPIP1) in the skin of patients suffering from HS. Skin biopsies of 15 patients with HS and 15 healthy controls were obtained and processed for immunohistochemistry, western blot, and real time PCR. The highest mean MCPIP1 mRNA expression was found in the inflammatory lesional skin of HS patients. It was significantly higher than MCPIP1 mRNA expression in the biopsies from both healthy controls and non-lesional skin of HS patients. Western blot analysis indicated that expression of MCPIP1 was elevated within both lesional and non-lesional skin compared to the healthy control. The increased MCPIP1 mRNA and protein expression level in HS lesions may indicate its possible role in the disease pathogenesis.
MSI Analysis in Solid and Liquid Biopsies of Gastroesophageal Adenocarcinoma Patients: A Molecular Approach
Authors: Boldrin Elisa , Piano Maria Assunta , Alfieri Rita , Mazza Marcodomenico , Vassallo Loretta , Scapinello Antonio , Pilati Pierluigi , Curtarello Matteo
Published: July 2021

Gastroesophageal adenocarcinoma (GEA) patients with the microsatellite instability (MSI) subtype emerged as optimal candidates for immunotherapy. To date, immunohistochemistry (IHC) is the gold standard for MSI assessment in formalin-fixed paraffin-embedded (FFPE) specimens. However, IHC, although useful for diagnostic typing, cannot be used to analyze cell-free DNA (cfDNA) in liquid biopsy, a tool that could overcome tumor heterogeneity and enable longitudinal monitoring. In order to find an alternative diagnostic method to IHC, we analyzed 86 retrospective GEAs FFPE samples with multiplex PCR. Moreover, to verify the feasibility of MSI detection in liquid biopsy, cfDNA samples of five patients that resulted in having MSI in a prospective cohort of 35 patients were evaluated by multiplex PCR, real-time PCR and droplet digital PCR (ddPCR). Analysis of FFPE showed 100% concordance between multiplex PCR and IHC (Cohen’s Kappa agreement = 1). On the contrary, only ddPCR was able to detect MSI in cfDNAs of T3/T4 GEA patients. In conclusion, data highlight the molecular analysis as an optimal alternative to IHC for the diagnostic typing and suggest that the ddPCR assay can be considered as the most reliable and promising molecular approach to detect MSI in the cfDNA of GEA patients.
The Receptor-Like Kinase ERECTA Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density
Authors: Li Huiguang , Yang Yanli , Wang Houling , Liu Sha , Jia Fuli , Su Yanyan , Li Shuang , He Fang , Feng Conghua , Niu Mengxue , Wang Jie , Liu Chao , Yin Weilun , Xia Xinli
Published: July 2021

Poplar is one of the most important tree species in the north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression of the PdERECTA gene, which is a member of the LRR-RLKs family from Populus nigra × (Populus deltoides × Populus nigra), improves water use efficiency and enhances drought tolerance in triploid white poplar. PdERECTA localizes to the plasma membrane. Overexpression plants showed lower stomatal density and larger stomatal size. The abaxial stomatal density was 24–34% lower and the stomatal size was 12–14% larger in overexpression lines. Reduced stomatal density led to a sharp restriction of transpiration, which was about 18–35% lower than the control line, and instantaneous water use efficiency was around 14–63% higher in overexpression lines under different conditions. These phenotypic changes led to increased drought tolerance. PdERECTA overexpression plants not only survived longer after stopping watering but also performed better when supplied with limited water, as they had better physical and photosynthesis conditions, faster growth rate, and higher biomass accumulation. Taken together, our data suggest that PdERECTA can alter the development pattern of stomata to reduce stomatal density, which then restricts water consumption, conferring enhanced drought tolerance to poplar. This makes PdERECTA trees promising candidates for establishing more water use efficient plantations.
Selection and Optimization of Reference Genes for MicroRNA Expression Normalization by qRT-PCR in Chinese Cedar (Cryptomeria fortunei) under Multiple Stresses
Authors: Zhang Yingting , Xue Jinyu , Zhu Lijuan , Hu Hailiang , Yang Junjie , Cui Jiebing , Xu Jin
Published: July 2021

MicroRNA (miRNA) expression analysis is very important for investigating its functions. To date, no research on reference genes (RGs) for miRNAs in gymnosperms, including Cryptomeria fortunei, has been reported. Here, ten miRNAs (i.e., pab-miR159a, cln-miR162, cas-miR166d, pab-miR395b, ppt-miR894, cln-miR6725, novel1, novel6, novel14 and novel16) and three common RGs (U6, 5S and 18S) were selected as candidate RGs. qRT-PCR was used to analyse their expressions in C. fortunei under various experimental conditions, including multiple stresses (cold, heat, drought, salt, abscisic acid and gibberellin) and in various tissues (roots, stems, tender needles, cones and seeds). Four algorithms (delta Ct, geNorm, NormFinder and BestKeeper) were employed to assess the stability of candidate RG expression; the geometric mean and RefFinder program were used to comprehensively evaluate RG stability. According to the results, novel16, cln-miR6725, novel1 and U6 were the most stable RGs for studying C. fortunei miRNA expression. In addition, the expression of three target miRNAs (aly-miR164c-5p, aly-miR168a-5p and smo-miR396) was examined to verify that the selected RGs are suitable for miRNA expression normalisation. This study may aid further investigations of miRNA expression/function in the response of C. fortunei to abiotic stress and provides an important basis for the standardisation of miRNA expression in other gymnosperm species.
CO2-Sensitive Connexin Hemichannels in Neurons and Glia: Three Different Modes of Signalling?
Authors: Hill Emily , Dale Nicholas , Wall Mark J.
Published: July 2021

Connexins can assemble into either gap junctions (between two cells) or hemichannels (from one cell to the extracellular space) and mediate cell-to-cell signalling. A subset of connexins (Cx26, Cx30, Cx32) are directly sensitive to CO2 and fluctuations in the level within a physiological range affect their open probability, and thus, change cell conductance. These connexins are primarily found on astrocytes or oligodendrocytes, where increased CO2 leads to ATP release, which acts on P2X and P2Y receptors of neighbouring neurons and changes excitability. CO2-sensitive hemichannels are also found on developing cortical neurons, where they play a role in producing spontaneous neuronal activity. It is plausible that the transient opening of hemichannels allows cation influx, leading to depolarisation. Recently, we have shown that dopaminergic neurons in the substantia nigra and GABAergic neurons in the VTA also express Cx26 hemichannels. An increase in the level of CO2 results in hemichannel opening, increasing whole-cell conductance, and decreasing neuronal excitability. We found that the expression of Cx26 in the dopaminergic neurons in the substantia nigra at P7-10 is transferred to glial cells by P17-21, displaying a shift from being inhibitory (to neuronal activity) in young mice, to potentially excitatory (via ATP release). Thus, Cx26 hemichannels could have three modes of signalling (release of ATP, excitatory flickering open and shut and inhibitory shunting) depending on where they are expressed (neurons or glia) and the stage of development.
Neutrophil Extracellular Traps in Colorectal Cancer Progression and Metastasis
Authors: Khan Umama , Chowdhury Sabrina , Billah Md Morsaline , Islam Kazi Mohammed Didarul , Thorlacius Henrik , Rahman Milladur
Published: July 2021

Neutrophils form sticky web-like structures known as neutrophil extracellular traps (NETs) as part of innate immune response. NETs are decondensed extracellular chromatin filaments comprising nuclear and cytoplasmic proteins. NETs have been implicated in many gastrointestinal diseases including colorectal cancer (CRC). However, the regulatory mechanisms of NET formation and potential pharmacological inhibitors in the context of CRC have not been thoroughly discussed. In this review, we intend to highlight roles of NETs in CRC progression and metastasis as well as the potential of targeting NETs during colon cancer therapy.