bluuebunny/crossref_metadata_2025 huggingface dataset:
Probable tirofiban-induced thrombotic microangiopathy after stent thrombosis: a case report
Authors: Mattioli Maria , Paoli Giorgia , Cambò Benedetta , Bonura Rosario
Published: January 2023

Background

Glycoprotein (GP) IIb–IIIa inhibitors are antithrombotic drugs used in selected patients during and after percutaneous coronary interventions (PCIs), usually as a bail-out in the setting of no-reflow or thrombotic complications. A notorious life-threatening adverse effect of this drug class is immune-mediated drug-induced thrombocytopenia (DITP). Thrombotic microangiopathy (TMA) induced by GP IIb–IIIa inhibitors has never been reported.

Case summary

A 72-year-old woman admitted for anterior myocardial infarction treated with primary PCI and stent implantation underwent a first tirofiban infusion as a bail-out strategy. After a new procedure for stent thrombosis, she received a second tirofiban infusion and developed sudden severe thrombocytopenia (platelet count <20 000/µL). Tirofiban was stopped but no observed increase in platelet count. Acute kidney injury due to renal ischaemia and left ventricular thrombosis followed. Unexpectedly, evidence for haemolysis and schistocytosis at peripheral blood smear prompted a diagnosis of TMA. Plasma exchange was immediately started with evidence for initial increase in platelet count, but the patient died due to sudden haemodynamic and respiratory deterioration.

Discussion

Tirofiban is known to rarely cause immune-dependent DITP. However, it has never been associated with TMA. This case report not only describes the first case of probable tirofiban-induced TMA, but also highlights the importance of a systematic approach to severe thrombocytopenia, even in the setting of low platelet count from a known DITP-related drug. Treatment of TMA in the difficult context of recent myocardial infarction and stent thrombosis requires a complex interplay between cardiologist, haematologist, transfusionist, and nephrologist, carefully balancing thrombotic and haemorrhagic risk.
Electrical storm in hypertrophic cardiomyopathy: Cardiac magnetic resonance and sudden cardiac death risk stratification: a case report
Authors: Garcia Brás Pedro , Rosa Sílvia Aguiar , Portugal Guilherme , Oliveira Mário Martins
Published: January 2023

Background

Risk stratification for sudden cardiac death (SCD) is a key factor in the management of patients with hypertrophic cardiomyopathy (HCM). Cardiac magnetic resonance (CMR) has a unique role in the evaluation of HCM and offers superior diagnostic and prognostic information to assess the indication for a prophylactic implantable cardioverter-defibrillator (ICD).

Case summary

A 39-year-old patient with non-obstructive HCM with a low ESC HCM Risk-SCD score underwent a CMR revealing a left ventricular apical aneurysm and extensive late gadolinium enhancement; a prophylactic ICD was thus implanted. A month later, the patient was admitted in refractory electrical storm with over 50 appropriate ICD shocks due to sustained ventricular tachycardia. Despite anti-arrhythmic therapy and mechanical ventilation, the evolution was unfavourable with haemodynamic instability; veno-arterial extracorporeal membrane oxygenation was implanted. The patient was submitted to CMR-guided epicardial VT catheter ablation with complications of LV thrombus and severe pericardial effusion.

Discussion

This case details the complex risk stratification for SCD in patients with HCM, highlighting the important role of CMR in the integrated approach to risk stratification.
Proteinuria is independently associated with heart failure events in patients with atrial fibrillation: the Fushimi AF registry
Authors: Ikeda Syuhei , An Yoshimori , Iguchi Moritake , Ogawa Hisashi , Nakanishi Yumiko , Minami Kimihito , Ishigami Kenjiro , Aono Yuya , Doi Kosuke , Hamatani Yasuhiro , Yoshizawa Takashi , Ide Yuya , Fujino Akiko , Ishii Mitsuru , Masunaga Nobutoyo , Esato Masahiro , Tsuji Hikari , Wada Hiromichi , Hasegawa Koji , Abe Mitsuru , Akao Masaharu
Published: January 2023

Aims

Previous studies have shown that proteinuria is independently associated with the incidence of atrial fibrillation (AF), and is also associated with the incidence of cardiovascular events such as stroke and thromboembolism in patients with AF. However, the association of proteinuria with heart failure (HF) events in patients with AF remains unclear.

Methods and results

The Fushimi AF Registry is a community-based prospective study of patients with AF. Of the entire cohort of 4489 patients, 2164 patients had available data of proteinuria. We compared the clinical background and outcomes between patients with proteinuria (n = 606, 28.0%) and those without (n = 1558, 72.0%). Patients with proteinuria were older and had a higher prevalence of major co-morbidities. During the median follow-up of 5.0 years, the incidence rates of HF events (composite of cardiac death or HF hospitalization) were higher in patients with proteinuria than those without (4.1% vs. 2.1% person-year, P < 0.01). Multivariate analyses revealed that proteinuria was an independent risk factor of the incidence of HF events [adjusted hazard ratio (HR): 1.40, 95% confidence interval (CI): 1.13–1.74]. This association was consistent among the various subgroups, except for the age subgroup in which there was a significant interaction (P < 0.01) between younger (<75 years) (unadjusted HR: 3.03, 95% CI: 2.12–4.34) and older (≥75 years) patients (unadjusted HR: 1.59, 95% CI: 1.23–2.05).

Conclusion

Our community-based large prospective cohort suggests that proteinuria is independently associated with the incidence of HF events in Japanese patients with AF.
Benignant and malignant epidemiology among surgical resections for suspicious solitary lung cancer without preoperative tissue diagnosis
Authors: Armand Elsa , Boulate David , Fourdrain Alex , Nguyen Ngoc-Anh-Thu , Resseguier Noémie , Brioude Geoffrey , Trousse Delphine , Doddoli Christophe , D’journo Xavier-Benoit , Thomas Pascal-Alexandre
Published: January 2023

OBJECTIVES

The aim of this study was to describe the epidemiology of patients undergoing diagnostic and/or curative surgical pulmonary resections for lung opacities suspected of being localized primary lung cancers without preoperative tissue confirmation.

METHODS

We performed a single-centre retrospective study of a prospectively implemented institutional database of all patients who underwent pulmonary resection between January 2010 and December 2020. Patients were selected when surgery complied with the Fleischner society guidelines. We performed a multivariable logistic regression to determine the preoperative variables associated with malignancy.

RESULTS

Among 1392 patients, 213 (15.3%) had a final diagnosis of benignancy. We quantified futile parenchymal resections in 29 (2.1%) patients defined by an anatomical resection of >2 lung segments for benign lesions that did not modified the clinical management. Compared with patients with malignancies, patients with benignancies were younger (57.5 vs 63.9 years, P < 0.001), had lower preoperative risk profile (thoracoscore 0.4 vs 2.1, P < 0.001), had a higher proportion of wedge resection (50.7% vs 12.2%, P < 0.01) and experienced a lower burden of postoperative complication (Clavien–Dindo IV or V, 0.4% vs 5.6%, P < 0.001). Preoperative independent variables associated with malignancy were (adjusted odd ratio [95% confident interval]) age 1.02 [1.00; 1.04], smoking (year-pack) 1.005 (1.00; 1.01), history of cardiovascular disease 2.06 [1.30; 3.30], history of controlled cancer 2.74 [1.30; 6.88] and clinical N involvement 4.20 [1.11; 37.44].

CONCLUSIONS

Futile parenchymal lung resection for suspicious opacities without preoperative tissue diagnosis is rare (2.1%) while surgery for benign lesions represented 15.3% and has a satisfactory safety profile with very low postoperative morbi-mortality.
Temporal changes in the surgical management of patients with tetralogy of Fallot in Denmark: a nationwide cohort study
Authors: Gröning Mathis , Smerup Morten Holdgaard , Nielsen Dorte Guldbrand , Nissen Henrik , Munk Kim , Mortensen Ulrik Markus , Andersen Helle , Engholm Morten , Bjerre Jesper , Vejlstrup Niels , Juul Klaus , Søndergaard Eva Vad , Jensen Annette Schophuus , Jørgensen Troels Højsgaard , Thyregod Hans Gustav Hørsted , Andersen Henrik Ørbæk , Jøns Christian , Helvind Morten , Sondergaard Lars
Published: January 2023

OBJECTIVES

To assess temporal changes in the surgical management of patients with tetralogy of Fallot including the timing of interventions, surgical techniques, reinterventions and survival in a nationwide cohort.

METHODS

Patients with tetralogy of Fallot in Denmark were divided into 3 eras based on their year of birth: early (1977–1991), intermediate (1992–2006) and late (2007–2021).

RESULTS

The cohort consisted of 745 patients. Median follow-up was 21.2 years (13.7–30.5). There was a temporal trend towards less shunt palliation (–0.3% per year, 95% CI –0.05 to –0.1). Median age at intracardiac repair was 2.9 years (1.8–5.0), 0.8 years (0.5–1.3) and 0.5 years (0.4–0.7) (P < 0.001) in the early, intermediate and late era, respectively. There was a temporal trend towards less valve-sparing repair (–0.7% per year, 95% CI –0.5 to –1.0) and more repair with transannular patches (0.7% per year, 95% CI 0.5–1.0). Survival at 10 years was 79% (64–76), 90% (87–93) and 95% (92–98) (P < 0.001) and pulmonary valve replacement within the first 10 years after intracardiac repair was performed in 3% (1–6), 12% (8–16) and 21% (13–29) (P < 0.001) in the early, intermediate and late era, respectively.

CONCLUSIONS

There was a temporal trend towards less shunt palliation and intracardiac repair at a younger age with more use of transannular patches. While survival throughout childhood and adolescence has improved, more patients undergo pulmonary valve replacement during the first 10 years after intracardiac repair.
Practising critical visual literacy through redesign in ELT classrooms
Authors: Brown Cecilie Waallann , Savić Milica
Published: January 2023

The aim of this article is to argue for the relevance of critical visual literacy (CVL) in relation to intercultural learning in ELT and present a redesign task that has been implemented with secondary school EFL learners in Norway. CVL is an approach to reading images which acknowledges that all texts are constructed, rather than neutral, and that they work to position the reader to accept a particular view of the world. Redesign tasks encourage learners to engage critically with these positions and create alternative texts that better reflect their personal worldviews. Through presenting examples of learner redesigns of a multimodal advertisement created in a different cultural context, we discuss how the task facilitated critical reading, exploration of multiple perspectives and engagement with sociopolitical issues. Such critical reading skills are crucial for navigating the increasingly more complex visual and intercultural landscapes the learners encounter in their everyday lives.
Teachers’ digital technology use after a period of online teaching
Authors: Moorhouse Benjamin Luke
Published: January 2023

This article reports on a study that investigated primary-school English-language teachers’ use of digital technologies in their in-person classrooms after a period of online teaching necessitated by the COVID-19 pandemic. It also explored the perceived effects the period of online teaching had on the teachers’ practices. Data were collected through in-depth interviews with sixteen experienced Hong Kong primary-school English-language teachers approximately one year after they returned to in-person teaching. The findings suggest that the period of online teaching has led to a greater utilization of technology for teaching. In addition, they suggest digital technologies are now embedded in teachers’ regular teaching practices, with the digital technologies adopted primarily those that enhance existing practices. Common digital technologies include: (1) learning management systems; (2) digital noticeboards; (3) interactive digital worksheets and quizzes; and (4) interactive presentation software. Examples of how teachers use digital technologies, and considerations for their use, are provided.
Artificial intelligence to enhance clinical value across the spectrum of cardiovascular healthcare
Authors: Gill Simrat K , Karwath Andreas , Uh Hae-Won , Cardoso Victor Roth , Gu Zhujie , Barsky Andrey , Slater Luke , Acharjee Animesh , Duan Jinming , Dall'Olio Lorenzo , el Bouhaddani Said , Chernbumroong Saisakul , Stanbury Mary , Haynes Sandra , Asselbergs Folkert W , Grobbee Diederick E , Eijkemans Marinus J C , Gkoutos Georgios V , Kotecha Dipak ,   BigData@Heart Consortium and the cardAIc group, Bunting Karina V , Tica Otilia , Mobley Alastair R , Wang Xiaoxia , Champsi Asgher , Haider Nafeesah Ahmad , Ventura Maximina , Young Alice , McGreavy Paul , Castellani Gastone , Bradlow William , O'Regan Declan , Center Julius
Published: January 2023

Artificial intelligence (AI) is increasingly being utilized in healthcare. This article provides clinicians and researchers with a step-wise foundation for high-value AI that can be applied to a variety of different data modalities. The aim is to improve the transparency and application of AI methods, with the potential to benefit patients in routine cardiovascular care. Following a clear research hypothesis, an AI-based workflow begins with data selection and pre-processing prior to analysis, with the type of data (structured, semi-structured, or unstructured) determining what type of pre-processing steps and machine-learning algorithms are required. Algorithmic and data validation should be performed to ensure the robustness of the chosen methodology, followed by an objective evaluation of performance. Seven case studies are provided to highlight the wide variety of data modalities and clinical questions that can benefit from modern AI techniques, with a focus on applying them to cardiovascular disease management.

Despite the growing use of AI, further education for healthcare workers, researchers, and the public are needed to aid understanding of how AI works and to close the existing gap in knowledge. In addition, issues regarding data access, sharing, and security must be addressed to ensure full engagement by patients and the public. The application of AI within healthcare provides an opportunity for clinicians to deliver a more personalized approach to medical care by accounting for confounders, interactions, and the rising prevalence of multi-morbidity.
Causal associations of sleep apnea, snoring with cardiovascular diseases, and the role of body mass index: a two-sample Mendelian randomization study
Authors: Wang Jiao , Campos Adrian I , Rentería Miguel E , Xu Lin
Published: January 2023

Aims

Previously, observational studies have identified associations between sleep apnea (SA) and cardiovascular diseases (CVDs), whereas whether the associations are causal remain unclear.

Methods and results

We used the bi-directional, two-sample Mendelian randomization (MR) study to assess the existence and direction of the causal relationship between SA or snoring and CVDs. Multivariable MR (MVMR) was used to assess the direct effect of SA on CVDs after adjusting for body mass index (BMI). Single-nucleotide polymorphisms (SNPs) associated with SA and snoring were obtained from the latest genome-wide association study, which combined five cohorts with a total number of 25 008 SA cases and 172 050 snoring cases (total = 523 366). Among the analytic sample of 523 366 individuals (25 008 SA cases and 172 050 snoring cases), and after correcting for multiple testing, inverse-variance weighted (IVW) showed that SA and snoring increased the risk of hypertension [odds ratio (OR) = 1.03, 95% CI 1.02–1.05 and 1.05, 1.03–1.07], and coronary artery disease (CAD) (1.41,1.19–1.67 and 1.61,1.26–2.07) with all false-discovery rate (FDR) < 0.05, but such associations were decreased dramatically after adjusting for BMI using MVMR-IVW (0.06 < FDRBMI adjusted < 0.20). SA and snoring were not associated with atrial fibrillation (AF), heart failure (HF), or stroke. The presence of hypertension may increase the risk of SA (1.53, 1.04–2.25), but this association did not pass multiple comparisons (FDR > 0.05).

Discussion

Our results suggest that SA and snoring increased the risk of hypertension and CAD, and these associations may partly be driven by BMI. Conversely, no evidence of CVDs causally influencing SA or snoring was found.
Constraints to speciation despite divergence in an old haplochromine cichlid lineage
Authors: Müller Claudio , Moser Florian N , Frei David , Seehausen Ole
Published: January 2023

Most of the 500+ cichlid species of Lake Victoria evolved very rapidly in the wake of an adaptive radiation within the last 15,000 years. All 500 species have evolved from just one out of five old cichlid lineages that colonized the lake. Endemic to the Lake Victoria region, Astatoreochromis alluaudi is a member of an old haplochromine lineage that never speciated in the region. Even though the species occurs in a wide range of habitats, there were no indications of evolutionary diversification. Here, we tested predictions of several hypothetical mechanisms that might constrain speciation, including high dispersal rates, a generalist life style and the lack of behavioral assortative mating. Genomic analyses of individuals from 13 populations revealed several genomically distinct groups, associated with major habitat classes, indicating the existence of two distinct ecotypes. We found significant phenotypic differences between these ecotypes in the wild, which were retained under common-garden conditions, potentially indicating heritable phenotypic adaptations. Female mate choice experiments revealed the absence of behavioral assortative mating despite genetic and phenotypic differentiation between ecotypes. We suggest that the lack of coupling between behavioral mating preferences and phenotypic and genetic divergence constrains speciation in this cichlid.
Management of diabetes patients with comorbidity in primary care: a mixed-method study in Odisha, India
Authors: Pati Sandipana , van den Akker Marjan , Schellevis F (François) G , Sahoo Krushna Chandra , Burgers Jako S
Published: January 2023

Background

Diabetes patients with comorbidities need regular and comprehensive care for their disease management. Hence, it is essential to assess the primary care preparedness for managing diabetes patients and the perspectives of the diabetes patients on the care received at the primary care facilities.

Methods

All 21 Urban Primary Health Centres (UPHCs) in Bhubaneswar city of Odisha, India, were assessed using the modified Primary Care Evaluation Tool and WHO Package of Essential Non-communicable disease interventions questionnaire. Additionally, 21 diabetes patients with comorbidities were interviewed in-depth to explore their perception of the care received at the primary care facilities.

Results

All the UPHCs had provisions to meet the basic requirements for the management of diabetes and common comorbidities like hypertension. There were few provisions for chronic kidney illness, cardiovascular disease, mental health, and cancer. Diabetes patients felt that frequent change in primary care physicians at the primary care facilities affected their continuity of care. Easy accessibility, availability of free medicines, and provisions of basic laboratory tests at the facilities were felt to be necessary by the diabetes patients.

Conclusion

Our study highlights the existing gaps in India’s healthcare system preparedness and the needs of diabetes patients with comorbidity. The government of India’s Health and Wellness (HWC) scheme aims to deliver comprehensive healthcare to the population and provide holistic care at the primary care level for NCD patients. It is imperative that there is an early implementation of the various components of the HWC scheme to provide optimal care to diabetes patients.
Validation of a diagnostic prediction tool for colorectal cancer: a case–control replication study
Authors: Nemlander Elinor , Rosenblad Andreas , Abedi Eliya , Hasselström Jan , Sjövall Annika , Carlsson Axel C , Ewing Marcela
Published: January 2023

Background

Early detection of colorectal cancer (CRC) is crucial for survival. Primary care, the first point of contact in most cases, needs supportive risk assessment tools. We aimed to replicate the Swedish Colorectal Cancer Risk Assessment Tool (SCCRAT) for non-metastatic CRC in primary care and examine if risk factor patterns depend on sex and age.

Methods

2,920 adults diagnosed with non-metastatic CRC during the years 2015–2019 after having visited a general practitioner the year before the diagnosis were selected from the Swedish Cancer Register and matched with 11,628 controls, using the same inclusion criteria except for the CRC diagnosis. Diagnostic codes from primary care consultations were collected from a regional health care database. Positive predictive values (PPVs) were estimated for the same 5 symptoms and combinations thereof as in the baseline study.

Results

The results for patients aged ≥50 years old in the present study were consistent with the results of the SCCRAT study. All symptoms and combinations thereof with a PPV >5% in the present study had a PPV >5% in the baseline study. The combination of bleeding with abdominal pain (PPV 9.9%) and bleeding with change in bowel habit (PPV 7.8%) were the highest observed PPVs in both studies. Similar risk patterns were seen for all ages and when men and women were studied separately.

Conclusion

This external validation of the SCCRAT for non-metastatic CRC in primary care replicated the baseline study successfully and identified patients at high risk for CRC.
Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600
Authors: Oyarzun Mejia Alejandra P , Hyman Michael R
Published: January 2023

An activity-based labeling (ABL) approach was investigated for the phenol-oxidizing bacterium, Pseudomonas sp. CF600. Phenol-grown cells were exposed to several different terminal diynes, and following cell breakage, extracts of these cells were added to copper-catalyzed alkyne/azide cycloaddition reactions containing Alexa Fluor 647 azide. Analysis of total cell proteins by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and near-infrared scanning demonstrated covalent fluorescent labeling of a 58- and a 34-kDa polypeptide in all diyne-treated cell types. Further studies using 1,4-diethynylbenzene (DEB) demonstrated that these labeled polypeptides were consistently detected in cells grown on substrates that exhibited phenol-dependent O2 uptake activity but not observed when cells were grown on substrates such as dextrose or catechol that did not support this activity. Fluorescent labeling of the two polypeptides in DEB-treated, phenol-grown cells was time dependent and was inhibited by several known substrates for phenol hydroxylase. These results suggest that diverse diynes act as mechanism-based inactivators of phenol hydroxylase in Pseudomonas sp. CF600 and that this effect can be exploited by ABL approaches to selectively label the major 58- and 34-kDa subunits of the hydroxylase component of this complex enzyme.
Taper equations for eight major forest tree species in flat land Ukraine
Authors: Myroniuk Viktor , Bilous Andrii , Lakyda Petro , Lesnik Oleksandr , Burianchuk Maksym , Svynchuk Viktor , Bychenko Volodymyr , Tyshchenko Oleksandr , Zadorozhniuk Roman , Soshenskyi Oleksandr , Matushevych Liubov , Diachuk Petro , Bala Oleksandr , Smolin Vitaliy , Yaroshchuk Mykola , Hrytsenko Oleksandr , Matsala Maksym
Published: January 2023

Stem taper equations are crucial for forest management allowing to reliably estimate merchantable wood volume. Their main benefit is the ability to predict stem diameters at a certain height of the stem. Ukraine has recently adopted European Union standards for round wood classification, which prompted the necessity to model stem taper and updates all reference data to conform with the new standards. This study is a systematic attempt to develop a set of taper equations for the most common forest tree species in the Polissia and Forest steppe of Ukraine. For this purpose, we used a data set of 1994 sample trees representing eight tree species collected on 238 sample plots. The Kozak A. (2004, My last words on taper equations. For. Chron.  80, 507–515) model was chosen to fit the taper equations. To characterize the variability in stem shape among tree species, mixed-effect models were calibrated for this equation. In this model, random-effect parameters were selected based on their coefficients of variation through a bootstrapping process. This is a novel feature we suggest for the process of calibrating taper models. The Kozak A. (2004, My last words on taper equations. For. Chron.  80, 507–515) equation showed a good performance in predicting diameters outside bark and estimating the total stem volume. Our mixed-effect modelling approach accurately characterizes the variation in stem form for different tree species based on adequately chosen random-effect parameters. The stem volumes derived from the developed taper models were compared with existing volume equations outputs (divergence up to 0.5 per cent). A deviation up to 5 per cent was found between the values of fitted and observed cylindrical form factors for the studied tree species. We expect that our taper equations will complement the future steps towards the development of reliable merchantable volume distribution models for the main tree species in the forests of flat land Ukraine, thus, contributing to transparency, reliability and sustainability of forest management and markets in Europe.
Evaluating the impact of prescribed surface fire on seedlings in the Central Hardwood Region, USA
Authors: Cuprewich Sarah A , Saunders Mike R
Published: January 2023

Oak (Quercus) is being successionally replaced by maple (Acer) across much of the eastern deciduous forest. Past research on the close relationship between oak and fire has led forest managers to use prescribed surface fires to deter this replacement. However, there has not been a comprehensive evaluation of the effectiveness of prescribed fire by managers, particularly in the Central Hardwood Region. For example, it is not known how many repeat applications of prescribed fire are needed to accumulate enough oak reproduction to successfully re-establish oak-dominated stands. Tree reproduction and midstory composition were systematically surveyed across 63 mature, oak-dominated stands ranging in prescribed fire histories and aspects on the Hoosier and Wayne National Forests in southern Indiana and Ohio, respectively. These stands represent the vast majority of stands burned to promote oak regeneration since 1990 in these two National Forests. Across all sites, seedlings in the white oak group (section Quercus) and red oak group (section Lobatae) comprised 47.8 ± 3.3 per cent and 8.2 ± 1.6 per cent (mean ± SE), respectively, of all seedlings. In comparison, common competing species (maples and American beech) comprised 5.4 ± 1.4 per cent of all seedlings. Mid- and overstory basal area, percent slope, years since last burn, and total number of burns significantly affected oak seedling density. Greater increases in taller, competitive oak seedling density occurred after sites had been burned three times and with high frequency (≤4 years between burns). However, results also suggest that if an established midstory already exists or fire has not been used on the landscape for almost a decade, repeat applications of prescribed fire will not likely increase oak regeneration in the short term without concurrent treatment of midstory stems.
Effects of lactic acid bacteria fermentation on the bioactive composition, volatile compounds and antioxidant activity of Huyou (Citrus aurantium‘Changshan-huyou’) peel and pomace
Authors: He Yake , Zhu Yanyun , Lv Jimin , Gu Ye , Wang Ting , Chen Jianchu
Published: January 2023

Using lactic acid bacteria (LAB) to ferment Huyou peel and pomace could provide a reference for making full use of Huyou resources and provide valuable suggestions for the industrial production of functional foods. The effects of LAB fermentation on the bioactive composition, volatile compounds and antioxidant activity of Huyou peel and pomace were studied. Results showed that both Huyou peel and pomace were good substrates for LAB growth. LAB had remarkable influence on bioconversion of organic acids, among which ascorbic acid content was increased significantly. In addition, LAB could produce flavonoids with enhanced bioactivity and absorption. In particular, the contents of rhoifolin, quercitrin and quercetin were increased by over 100%. Besides, the volatile compounds contents and aroma complexity were improved. Furthermore, the antioxidant activity was enhanced. Thus, our study provides high-value applications of Huyou, and fermented products with improved nutritional value and flavor are potentially beneficial to human health.
Re-recognition of BMPR1A-related polyposis: beyond juvenile polyposis and hereditary mixed polyposis syndrome
Authors: Zhao Zi-Ye , Lei Ye , Wang Zhao-Ming , Han Huan , Xing Jun-Jie , Xu Xiao-Dong , Gao Xian-Hua , Zhang Wei , Yu En-Da
Published: January 2023

Background

Bone morphogenetic protein receptor type 1A (BMPR1A) is responsible for two individual Mendelian diseases: juvenile polyposis syndrome and hereditary mixed polyposis syndrome 2, which have overlapping phenotypes. This study aimed to elucidate whether these two syndromes are just two subtypes of a single syndrome rather than two isolated syndromes.

Methods

We sequenced the BMPR1A gene in 186 patients with polyposis and colorectal cancer, and evaluated the clinicopathological features and phenotypes of the probands and their available relatives with BMPR1A mutations.

Results

BMPR1A germline mutations were found in six probands and their three available relatives. The numbers of frameshift, nonsense, splice-site, and missense mutations were one, one, two, and two, respectively; two of the six mutations were novel. Typical juvenile polyps were found in only three patients. Two patients had colorectal cancer rather than any polyps.

Conclusions

Diseases in BMPR1A germline mutation carriers vary from mixed polyposis to sole colorectal cancer, and typical juvenile polyps do not always occur in these carriers. The variety of phenotypes reflected the features of BMPR1A-mutation carriers, which should be recognized as a spectrum of one syndrome. Genetic testing may be a good approach to identifying BMPR1A-related syndromes.
Qualitative and quantitative comparison of the genetic and hybrid genetic algorithm to estimate acoustic impedance from post-stack seismic data of Blackfoot field, Canada
Authors: Maurya S P , Singh R , Mahadasu P , Singh U P , Singh K H , Singh R , Kumar R , Kushwaha P K
Published: January 2023

In this study, seismic inversion is carried out using a genetic algorithm (GA) as well as a hybrid genetic algorithm (HGA) approach to optimize the objective function designed for the inversion. An HGA is a two steps coupled process, where a local optimization algorithm is applied to the best model obtained from each generation of the GA. The study aims to compare the qualitative as well as the quantitative performance of both methods to delineate the reservoir zone from the non-reservoir zone. Initially, the developed algorithm is tested on synthetic data followed by its application to real data. It is found that the HGA for synthetic data is providing more accurate and high-resolution subsurface information as compared with the conventional GA although the time taken later is less as compared with the former methods. The application to real data also shows very high-resolution subsurface acoustic impedance information. The interpretation of the impedance section shows a low impedance anomaly zone at (1055–1070) ms time interval with impedance ranging from (7500 to 9500) m s−1*g cc−1. The correlation between seismic and well data shows that the low impedance zone is characterized as a clastic glauconitic sand channel (reservoir zone). In seismic inversion using an HGA, one can delineate the areal extent of the reservoir zone from the non-reservoir zone more specifically as compared to the GA-derived impedance. The convergence time of HGA is 4.4 per cent more than GA and can be even more for larger seismic reflection data sets. Further, for a more detailed analysis of the reservoir zone and to cross-validate inverted results, an artificial neural network (ANN) is applied to data, and porosity volume is predicted. The analysis shows that the low impedance zone interpreted in inversion results are correlating with the high porosity zone found in ANN methods and confirm the presence of the glauconitic sand channel. This study is important in the aspect of qualitative as well as quantitative comparison of the performance of the GA and HGA to delineate sand channels.
The influence of physical and algorithmic factors on simulated far-field waveforms and source–time functions of underground explosions using unsupervised machine learning
Authors: Harding Jennifer L , Preston Leiph A , Eliassi Mehdi
Published: January 2023

Characterizing explosion sources and differentiating between earthquake and underground explosions using distributed seismic networks becomes non-trivial when explosions are detonated in cavities or heterogeneous ground material. Moreover, there is little understanding of how changes in subsurface physical properties affect the far-field waveforms we record and use to infer information about the source. Simulations of underground explosions and the resultant ground motions can be a powerful tool to systematically explore how different subsurface properties affect far-field waveform features, but there are added variables that arise from how we choose to model the explosions that can confound interpretation. To assess how both subsurface properties and algorithmic choices affect the seismic wavefield and the estimated source functions, we ran a series of 2-D axisymmetric non-linear numerical explosion experiments and wave propagation simulations that explore a wide array of parameters. We then inverted the synthetic far-field waveform data using a linear inversion scheme to estimate source–time functions (STFs) for each simulation case. We applied principal component analysis (PCA), an unsupervised machine learning method, to both the far-field waveforms and STFs to identify the most important factors that control variance in the waveform data and differences between cases. For the far-field waveforms, the largest variance occurs in the shallower radial receiver channels in the 0–50 Hz frequency band. For the STFs, both peak amplitude and rise times across different frequencies contribute to the variance. We find that the ground equation of state (i.e. lithology and rheology) and the explosion emplacement conditions (i.e. tamped versus cavity) have the greatest effect on the variance of the far-field waveforms and STFs, with the ground yield strength and fracture pressure being secondary factors. Differences in the PCA results between the far-field waveforms and STFs could possibly be due to near-field non-linearities of the source that are not accounted for in the estimation of STFs and could be associated with yield strength, fracture pressure, cavity radius and cavity shape parameters. Other algorithmic parameters are found to be less important and cause less variance in both the far-field waveforms and STFs, meaning algorithmic choices in how we model explosions are less important, which is encouraging for the further use of explosion simulations to study how physical Earth properties affect seismic waveform features and estimated STFs.
Reply to comment on Geophysical inversion and Optimal Transport, 231, 172–198, by Okazaki & Ueda
Authors: Sambridge Malcolm , Jackson Andrew , Valentine Andrew P
Published: January 2023

Concerns raised by Okazaki & Ueda (2022) on the paper by Sambridge et al. (2022) are addressed. Two issues are discussed and some new numerical results presented. The first concerns whether the properties of the Wasserstein time-series misfit introduced in our earlier paper will translate to model space non-uniqueness in a seismic waveform inversion setting. It is argued that this is unlikely, given the special conditions, which must exist between all observed/predicted seismic waveform pairs for non-uniqueness to result. The second issue discussed is the efficacy of using the Sliced Wasserstein algorithm of Bonneel et al. (2015) as an alternate to the marginal Wasserstein algorithm, as proposed by Okazaki & Ueda (2022). It is argued that for optimization-based waveform fitting, the Sliced Wasserstein algorithm is a viable alternate provided care is taken to ensure that conditions arise which do invalidate analytical derivative expressions of the resulting Wasserstein misfit. In practice, this would likely mean recasting the 2D Optimal Transport problem posed in our earlier paper onto unstructured grids.
Key changes in bovine milk immunoglobulin G during lactation: NeuAc sialylation is a hallmark of colostrum immunoglobulin GN-glycosylation
Authors: Gazi Inge , Reiding Karli R , Groeneveld André , Bastiaans Jan , Huppertz Thom , Heck Albert J R
Published: January 2023

We monitored longitudinal changes in bovine milk IgG in samples from four cows at 9 time points in between 0.5 and 28 days following calving. We used peptide-centric LC–MS/MS on proteolytic digests of whole bovine milk, resulting in the combined identification of 212 individual bovine milk protein sequences, with IgG making up >50 percent of the protein content of every 0.5 d colostrum sample, which reduced to ≤3 percent in mature milk. In parallel, we analyzed IgG captured from the bovine milk samples to characterize its N-glycosylation, using dedicated methods for bottom-up glycoproteomics employing product ion-triggered hybrid fragmentation; data are available via ProteomeXchange with identifier PXD037755. The bovine milk IgG N-glycosylation profile was revealed to be very heterogeneous, consisting of >40 glycoforms. Furthermore, these N-glycosylation profiles changed substantially over the period of lactation, but consistently across the four individual cows. We identified NeuAc sialylation as the key abundant characteristic of bovine colostrum IgG, significantly decreasing in the first days of lactation, and barely detectable in mature bovine milk IgG. We also report, for the first time to our knowledge, the identification of subtype IgG3 in bovine milk, alongside the better-documented IgG1 and IgG2. The detailed molecular characteristics we describe of the bovine milk IgG, and their dynamic changes during lactation, are important not only for the fundamental understanding of the calf’s immune development, but also for understanding bovine milk and its bioactive components in the context of human nutrition.
Barrier profiles in workplace health promotion in Germany
Authors: Noehammer Elisabeth , Amler Nadja , Fischmann Wolfgang
Published: January 2023

Lay Summary

Workplace health promotion (WHP) is often faced with low-participation rates despite high relevance. This limits the potential for creating positive effects for the organization and its staff. Therefore, we investigated the barriers perceived by employees themselves using a representative sample (regarding age, gender and education) in Germany. Data were collected using a quantitative online questionnaire and then analyzed regarding underlying patterns. We found that there are different barrier types, and their importance differs depending on demographic criteria of the participants and the organizations they work for. These results can help organizations to reduce participation barriers to WHP for their staff.
Qualitative exploration of the reasons for not using nutritional warnings after policy implementation in Uruguay
Authors: Ares Gastón , Antúnez Lucía , Curutchet María Rosa , Galicia Luis , Natero Virginia , Giménez Ana , Otterbring Tobias
Published: January 2023

Nutritional warnings have gained popularity, particularly in the region of the Americas, to facilitate the identification of products with excessive content of nutrients associated with non-communicable diseases and encourage healthier food choices. Although warnings have been shown to be effective, an in-depth understanding of the reasons why some consumers do not use them is still lacking. The aim of the present work was to explore self-reported use of nutritional warnings and to identify the reasons for not considering nutritional warnings for making food purchase decisions after policy implementation in Uruguay. A non-probabilistic sample of 858 Uruguayan participants was recruited using an advertisement on Facebook and Instagram. Through an online survey, self-reported use of nutritional warnings was asked using a closed-open ended questions. Participants who reported not considering warnings to make their purchase decisions were asked to explain the reasons why using an open-ended question. Responses were analysed using deductive coding, based on the Behavioural Drivers Model. Thirty seven percent of the participants stated that the warnings had not influenced their purchase decisions. Motives for not being influenced by the warnings were related to lack of interest, attitudes, lack of perceived self-efficacy, cognitive biases and limited rationality when making purchase decisions. In addition, structural barriers, such as availability, cost and trust in the food industry also emerged from participants’ responses. Strategies to encourage the use of warnings should include communication campaigns and policies to address structural barriers related to the perceived availability and affordability of healthy foods.
Attending sporting mega events during COVID-19: mitigation and messaging at UK EURO 2020 matches
Authors: Purves Richard I , Maclean Jordan , Rocha Claudio , Philpott Matthew , Fitzgerald Niamh , Piggin Joe , Hunt Kate
Published: January 2023

The UEFA EURO 2020 football tournament was one of the largest Sporting Mega Events (SMEs) to take place during the COVID-19 pandemic. Mitigating the risk of virus transmission requires a multi-layered approach for any large event, more so in this case due to staging the tournament across eleven host countries. Yet, little is known about COVID-19 risks and mitigation from attending an event of this scale and nature. We examined the implementation of mitigation and messaging at EURO 2020 matches hosted at venues in the UK. The tournament was postponed from the summer of 2020 and played in June and July of 2021. Structured observations were conducted by 11 trained fieldwork-supporters at 10 matches played at Wembley Stadium, London, or Hampden Park, Glasgow. Fieldwork-supporters observed one-way systems and signage, and hand sanitizing stations inside the stadia, but reported significant variation in the implementation of staggered timeslots, testing upon entry, and procedures for exit. Adherence to planned measures by ticket holders and implementation by stewards waned as the tournament progressed culminating in an absence of enforced measures at the final. The non-compliance with COVID-19 mitigation measures was likely to have led to a significantly increased risk of transmission. Future events should consider how COVID-19 mitigation measures could become ‘new norms’ of fan behaviour, learning from what is already known about football fandom. Tournament organizers of SMEs can use these findings to promote clearer messaging on pandemic-driven changes in fan behaviour and best practices in mitigating risk at future sporting and cultural events.
Health education in conservatoires: what should it consist of? Findings from workshops with experts (Part II)
Authors: Matei Raluca , Phillips Keith
Published: January 2023

Although health education programmes have been implemented in higher music education (HME) and their evaluations published in peer-reviewed journals, guidelines as to what ought to be included in these programmes are still missing. This study aimed to document expert discussions on the content of an ideal health education curriculum for HME students in the UK, integrating critical thinking. Four interdisciplinary workshops were conducted, where 67 experts in relevant fields took part, and were asked to discuss four lists of topics and concepts created based on literature reviews (cognitive biases, logical fallacies, critical appraisal tools and health topics). Only the list on health topics is relevant here. Notes taken by the participants and ourselves were thematically analysed. Four themes were identified, two of which are reported in this paper: (i) The health education curriculum and (ii) A settings-based approach to health. Part I of this project (published elsewhere) is focussed on the critical thinking content of health education for conservatoire students. The present paper focusses on the ideal health education curriculum and its implications for the wider context of health promotion.
Associations of health literacy with missed free influenza immunization in people with chronic diseases
Authors: Arsenović Sladjana , Trajković Goran , Pekmezović Tatjana , Gazibara Tatjana
Published: January 2023

There is a gap in understanding specific features of health literacy that might be associated with adherence to influenza immunization. The aim of this study was to examine the association of health literacy with avoidance of influenza immunization and beliefs about the influenza vaccine among people with chronic diseases. Selection of study participants was based on a case–control study design in a population of people with chronic illnesses living in the Foča region (Republic of Srpska, Bosnia and Herzegovina) and who were entitled to receive a free influenza shot in season 2017/2018. The cases represented all non-vaccinated people (a total of 146) and the controls were vaccinated people (a total of 149) matched according to town of residence. Participants were interviewed in person from April to December 2018 using a socio-demographic questionnaire, Health Literacy Questionnaire (HLQ) and Health Belief Model Applied to Influenza (HBMAI). Better score on ‘Feeling understood and supported by healthcare providers’, ‘Social support for health’ and ‘Ability to actively engage with healthcare providers’ was associated with more perceived benefits of influenza immunization in men, but not in women. Perceiving more Barriers to influenza immunization was associated with lower scores on ‘Feeling understood and supported by healthcare providers’, ‘Have sufficient information to manage health’, ‘Social support for health’ and ‘Ability to actively engage with healthcare providers’ in both men and women. Health literacy may be associated with the perception of susceptibility to influenza, benefits and barriers to influenza immunization and motivations to receive influenza vaccine.
Culture-centric narratives of influenza vaccination among high-risk groups in Hong Kong
Authors: Ka Lai Lee Danielle , Jiang Crystal Li
Published: January 2023

Despite the effectiveness of influenza vaccination, the vaccine coverage rate among high-risk groups in Hong Kong is less than optimal. Guided by the Health Belief Model (HBM) and culture-centric narrative approach, we examined the role of cultural specificities in implicit assumptions held by at-risk individuals when the individuals decided whether to receive the vaccine. Data were collected from 29 in-depth interviews with people from high-risk groups in Hong Kong. From their decision narratives, it is evident that the local socio-cultural characteristics and collectivistic ideology are useful in understanding the perceptions of influenza severity, susceptibility to infection, perceived barriers and benefits, and self-efficacy of accepting or rejecting the vaccine among the high-risk individuals. Implications of vaccination message designs are discussed.