bluuebunny/crossref_metadata_2025 huggingface dataset:
Induksi Mutasi Monstera adansonii untuk Menghasilkan Variegata Secara Ex Vitro
Authors: Suhaimi Annisa , Umam Khotibul , Sandra Edhy
Published: February 2023

Monstera merupakan salah satu tanaman hias yang saat ini banyak diminati oleh pecinta tanaman karena memiliki nilai estetika tersendiri. Ditengah pandemi covid19 melanda dunia termasuk Indonesia, tanaman yang dijadikan primadona oleh pebisnis tanaman hias adalah genus Monstera, salah satunya Monstera adansonii. M. adansonii yang diminati saat ini adalah jenis variegata. Salah satu teknik untuk menghasilkan variegata pada M. adansonii adalah dengan melakukan mutasi pada bagian daun sehingga menghasilkan daun berwarna putih belang berbeda dari warna aslinya. Pada penelitian ini dilakukan mutasi pada M. adansonii menggunakan  empat zat kimia yaitu streptomisin, strepson, etil metil sulfonat (EMS), dan ekstrak rokok kretek dengan tujuan untuk mengetahui mutagen yang paling efektif membentuk variegata pada M. adansonii. Setelah perlakuan diberikan, kemudian dilakukan pengamatan terhadap jumlah tunas, warna dan jumlah daun. M. adansonii dengan perlakuan streptomisin menghasilkan warna hijau bercorak putih pada daun, sedangkan zat mutagen lainnya strepson, EMS dan ekstrak rokok kretek tidak dapat menghasilkan variegata pada M. adansonii.
Potensi Trichoderma asperellum TKD dalam Menghambat Phytophthora spp. pada Benih Kakao Selama Masa Penyimpanan
Authors: Lila Kadek Dian Lila Sawitri Kumala , Meitini Wahyuni Proborini  , Febri Eka Wijayanti
Published: February 2023

Benih berkualitas sangat diperlukan dalam perbanyakan tanaman kakao. Adanya infeksi Phytophthora spp. menjadikan kualitas benih kakao menurun yang berdampak pada kemampuan tumbuh tanaman. Cendawan T. asperellum TKD dapat menghambat pertumbuhan patogen. Tujuan penelitian ini yaitu menguji kemampuan T. asperellum TKD dalam menghambat Phytophthora spp. secara in vitro dan in vivo, menentukan konsentrasi spora T. asperellum TKD yang optimum dan uji viabilitas benih. Perlakuan in vitro dilakukan dengan kultur ganda, dan perlakuan in vivo dilakukan pada benih kakao. Parameter yang diamati meliputi persentase daya hambat dengan kultur ganda, daya kecambah, indeks kecepatan perkecambahan dan penurunan populasi Phytophtora spp. Hasil penelitian menunjukan T. asperellum TKD menghambat Phytophthora spp. secara in vitro dan in vivo (P≤0,05). Rerata daya hambat in vitro adalah 58,74±4,58%. Konsentrasi spora T. asperellum TKD hingga 104 efektif menurunkan populasi Phytophthora spp secara in vivo. Konsentrasi spora T. asperellum TKD yang dapat menghambat pertumbuhan Phytophthora spp.  yang optimum adalah konsentrasi T1,70x106. Mekanisme penghambat T. asperellum TKD adalah kompetisi dimana terjadi interaksi dengan mekanisme mikoparasit dan antibiosis. Pemberian T. asperellum TKD menyebabkan benih memiliki daya perkecambahan lebih dari 80% dengan diikuti indeks kecepatan kecambah yang tinggi. Pemberian T. asperellum TKD mampu menghambat pertumbuhan Phytophthora spp. secara in vitro dan in vivo dan benih kakao dapat berkecambah optimal hingga hari ke-7 penyimpanan setelah pemberian T. asperellum TKD.
The role of endothelial protection in the treatment of patients with arterial hypertension: the effectiveness of quercetin
Authors: Prokosa M.I.
Published: February 2023

The article presents the results of a clinical study of the effectiveness of the cytoprotective drug quercetin as a part of combined antihypertensive therapy on indices of endothelial function and daily blood pressure profile. The aim of the study. To conduct a comparative assessment of the dynamics of indices in 24-hour blood pressure monitoring (BPM) and cytokine levels in patients with hypertension, depending on using quercetin in treatment.Materials and methods. 120 patients with stage II hypertension of 2–3 degrees (66 female and 54 male) were examined. Patients were divided into 2 groups: Group I (study) – 58 patients who took quercetin (Corvitin®) in addition to basic therapy, mean age 57.87±13.6 years; II group (comparison) – 62 patients who underwent only basic antihypertensive therapy (Ramipril/Amlodipine), mean age 59.09±12.47 years. The results. During the administration of quercetin along with the standard basic therapy with the combination of Ramipril/Amlodipine in patients with stage II hypertension of 2-3 degrees of severity, more pronounced significant positive changes in the major indices of 24-hour BPM were observed, an increase in the ratio of people with a “dipper” profile due to a decrease in the number of patients with an insufficient reduction in nocturnal BP, an excessive drop in nocturnal BP or its persistent increase. The additional administration of quercetin contributes to the more effective correction of endothelial dysfunction indices: it significantly reduces the levels of soluble vascular cell adhesion molecules (s-VCAM), and soluble intercellular adhesion molecules 1 (s-ICAM-1), ET-1, IL-1, IL-6, TNF-a. During the administration of basic therapy, changes in IL-1 and ET-1 levels were less pronounced and insignificant. Correlation analysis shows the close relationship between a decrease in the main indices of 24-hour BPM and a decrease in the concentration of the cytokines, which characterize the state of endothelial dysfunction and systemic inflammation.Conclusion. The use of quercetin reliably reduces the levels of cytokines, which ensures a reduction in the manifestations of ED and contributes to better control of all blood pressure indices and normalization of the daily profile of blood pressure.
The effect of endogenous antimicrobial peptides (HNPs1-3 and LL-37) on the course of duodenal ulcer in children
Authors: Moldovan P. , Sorokman T.V.
Published: February 2023

Introduction. Duodenal ulcer in children is characterized by a significant prevalence, frequent development of serious complications and the need for large financial allocations for the implementation of treatment programs. Antimicrobial peptides have an immunomodulatory function and can be used as markers of the severity of the inflammatory process.Aim. To investigate the concentration of HNPs 1-3 and LL-37 in children with duodenal ulcer.Methods. A study of 47 children with duodenal ulcer, aged 7–18 years, was conducted with the determination of the CagA HR antigen in feces by enzyme-linked immunosorbent assay, the levels of HNPs 1-3 and LL-37 in blood plasma by enzyme-linked immunosorbent assay on the "PrisMatic" IN013 model 4301 analyzer test systems "MyBioSource, Inc.", USA. All children underwent a complex clinical and instrumental laboratory examination (topographic intragastric pH-metry, endoscopic examination with targeted biopsy for morphological examination and determination of HP). Results. 76.6% of people with DU were infected with HP. 80% had a toxigenic CagA strain of HP. In the vast majority of children with DU, small ulcerative defects were diagnosed. II and III degrees of inflammatory activity occurred with almost the same frequency (44.6% and 34% of people), while I degree occurred only in 21.3% of cases. The average level of HNP1-3 in children with DU was 46.56 ± 8.52 ng/ml, LL-37 - 269.9 ± 26.53 ng/ml. Direct probable connections between the levels of HNP1-3 and LL-37 and the severity of the course and the activity of the inflammatory process were established.Conclusion. Elevated levels of LL-37 and HNPs 1-3 were found in the blood plasma of children with duodenal ulcers associated with CagA strains of Helicobacter pylori. Significantly higher levels of LL-37 and HNPs 1-3 were registered with a severe course of the disease and an active inflammatory process, which indicates a direct influence of antimicrobial peptides on the course of the pathological process.
History of the development of the lymphatic system (part one)
Authors: Kryvetskyi V.V. , Proniaiev D.V. , Protsak T.V. , Banul B.Y. , Yemelianenko N.R. , Voloshyn V.L.
Published: February 2023

The history of lymphatic system research goes back to ancient times. Lymph nodes were likely first mentioned in the hieroglyphs of ancient Egypt. Hippocrates (5th century BC) in the 5th century BC was one of the first to mention the lymphatic system. One of the first descriptions of what can be attributed to lymphatic vessels can be found in Aristotle. The Byzantine physician Pavlo Aeginsky was a famous surgeon who illustrated the tonsils and performed the first tonsillectomy, which allowed him to identify and describe infected cervical lymph nodes. Indian and Islamic medicine, especially Avicenna, gave interesting descriptions of lymphedema (elephant disease) due to frequent parasitic infections which are more common in eastern regions. Rufus of Ephesus, a Roman physician, discovered the axillary, inguinal, and mesenteric lymph nodes, as well as the thymus, in the 1st-2nd century AD. The first mention of lymphatic vessels was in the 3rd century BC by Herophilus, a Greek anatomist who lived in Alexandria. The Alexandrian school made significant contributions to the study of the lymphatic system stemming from the works of Galen. However, whether the structures described were lymphatic vessels is still debated. Erasistratus, during the dissection of a dairy lamb, showed that the abdominal arteries are filled with milk. Very likely, this is the first misinterpreted study of mesenteric lymphatic vessels. Based on the first observations made by the medieval Arab anatomist ibn Al-Nafis, the Spanish scientist and theologian Miguel Servetus, and the Italian anatomist Realdo Colombo, who described pulmonary circulation, and Andrea Cesalpino, who first introduced the term "circulation" in relation to the cardiovascular system, it was established basic regularities of the structure of the lymphatic system. In the middle of the 16th century, Gabriele Fallopio (researcher of fallopian tubes) described the vessels now known as "mammary glands". Based on all these discoveries, the Italian surgeon and anatomist Giovanni Guglielmo Riva was the first to present a graphic representation of the lymphatic system in two of his four oil paintings, which are now kept in the Academy of History "Arte Sanitaria" in Rome.
Bibliometric analysis of scientific literature on biopsy of sentinel lymph nodes in patients with thyroid gland cancer
Authors: Moskalenko Y.V. , Kurochkin A.V.
Published: February 2023

Biopsy of sentinel lymphatics has reliably entered the guidelines for diagnosing and treating many malignant neoplasms, but the situation in patients with thyroid cancer remains debatable.Objective. The purpose of the work is the search, bibliometric analysis, and generalization of scientific literature data to study the prognostic value and features of regional metastasis of highly differentiated forms of thyroid cancer, as well as the study of sentinel lymph node biopsy techniques.Materials and methods. Two hundred forty-two literature sources containing information on sentinel lymph node biopsy in patients with papillary and follicular thyroid cancer were examined. The data search was conducted in the electronic databases of PubMed, Scopus, Web of Science, and Google Scholar using the following key terms: "thyroid cancer," "sentinel lymph node biopsy," and "dyes." Using visualization tools and modern citation metrics SciVal (Scopus) with the application of an online platform for monitoring and analyzing international scientific research, it was possible to perform a bibliometric analysis. Bibliometric networks were constructed and visualized using VOSviewer.Results and conclusions. Regional metastasis can occur both in stages and with the omission of the predicted department. Lymph nodes of the ipsilateral central department occupy the first place in damage frequency.Regional metastasis is recognized as a negative prognostic factor for the development of locoregional recurrence of the disease. The mortality rate increases only in risk factors such as age <45 years, bulky thyroid tumor, and involvement of up to 6 metastatic lymph nodes. The main criterion for the effectiveness of the contrast during sentinel lymph node biopsy is the negative predictive value and the false negative rate. According to the bibliographic analysis, interest in sentinel lymph node biopsy in patients with thyroid cancer continues to grow from 1998 to the present. With the help of the VOSviewer bibliometric network visualization tool, it was possible to analyze 242 scientific articles.
Current state of the problem of forensic medical determination of the time since death (literature review)
Authors: Sokol V.K.
Published: February 2023

Determining the time since death is a key issue for forensic authorities when working with a corpse at the scene of death. After all, it is precisely the accurate data provided by the forensic medical expert that makes it possible to answer important questions during the investigation of a crime. Despite the enormous amount of fundamental research conducted over many decades and devoted to the exact establishment of the time since death, this problem has not been solved yet. Despite the obtained promising results, most of the proposed methods were characterized as unreliable due to sensitivity to the influence of many external and internal modifying factors. In our opinion, it is promising to combine different methods of postmortem interval diagnostics for the simultaneous analysis of several components, which will allow taking into account various causes of death, pathological conditions and other factors that can influence the obtained result.
Analysis of the manifestation frequency of different variants of calcifications in the mammary glands in women of mature and old age according to screening mammography data
Authors: Adamovych O.O. , Vasyliv M.A.L. , Masna Z.Z.
Published: February 2023

The relevance of the problem of early diagnosis of pathological changes in the mammary glands (MG) of women of different ages is due to the fact that in the world rankings of neoplasms localization, both benign and malignant, the MG occupies a leading position today. According to multiple information sources, more than 80% of women of reproductive age are diagnosed with various MG diseases. For the purpose of early detection of pathological changes developing in MG today, mammography is widely used, which allows easy visualization of calcifications, MG and obtaining their complete characteristics.The purpose of our study was to determine the frequency of detection and to study the age-related dynamics of different types of calcifications in the mammary glands of mature and elderly women according to the mammography results.Material and methods. In the course of the research, a randomized sample of archival anonymized mammograms of 100 women (50 mature and 50 elderly) who underwent screening or preventive examination was processed. Examinations were performed on the Fujifilm Amulet Innovality device (Japan) in direct (SS) and medial-lateral oblique (MLO) projections. Calcifications were assessed by shape, number, and size.Research results. It was determined that in a randomized sample of examined women, only 34% of adults and 28% of elderly people did not have calcifications in the mammary glands. In 24% of adults and 48% of elderly people, calcifications were detected in the mammary glands bilaterally, in another 42% of adults and 24% of elderly people - in only one of the the mammary glands. Among women of mature age, the largest specific share in the examined sample belongs to persons without calcifications in the mammary glands; among the elderly, the largest share is women with calcifications in both mammary glands. According to the results of the conducted research in a randomized sample of mature and elderly women, the following was found: isolated scattered punctate calcifications (35% of the examined); single punctate/round calcifications (23% of the examined); annular calcifications (11% of the examined); multiple calcifications (19% of the examined); calcifications larger than 1 mm (14% of the examined); calcified vessels (4% of the examined).Conclusions. Digital mammography is a highly informative method of examining the mammary glands, which allows us to visualize calcifications and differentiate them by size, shape and number. According to the results of the analysis of a randomized examination sample of 100 mature and elderly women, various variants of calcifications were found as single, diffuse, punctate; single punctate/round; annular; multiple; greater than 1 mm; as well as calcified vessels. With age, the frequency of mammary gland calcifications increases.
Forensic determination of the time of formation of damage to internal human organs using the method of reconstructing the optical activity of histological sections
Authors: Lytvynenko O.Y. , Vanchulyak O.Y.
Published: February 2023

Purpose of work. Development of a technique for determining the time of formation of damage to human internal organs by digital histological methods of polarization reconstruction of circular birefringence of molecular complexes.Materials and methods. The object of the study was the histology of samples of human internal organs (brain, kidney and liver) with different duration of damage from 1 to 120 hours. For control, we used biological tissue samples of those who died from coronary heart disease with different duration of damage from 1 to 120 hours. The studies were carried out using the technique of polarization reconstruction of circular birefringence of molecular complexes.Results. A set of diagnostically relevant relationships between temporal changes in the statistical structure of topographic maps of circular birefringence of optically active molecular complexes of histological sections of human internal organs with different duration of damage and variations in the mean value, dispersion, asymmetry and excess, characterizing the distribution of the value of this parameter of anisotropy, has been determined.Conclusions. A new original method has been developed for tomography of the optical activity of molecular complexes of tissues of human internal organs in a digital histological study of the age of damage to the tissues of the brain, liver and kidney, as well as the myocardium and lung tissue at a time interval of 1 up to 120 hours.
THE ROLE OF MONEY IN THE PURSUIT OF HAPPINESS
Authors: Stober Emmanuel Olusegun
Published: February 2023

Whosoever says money cannot buy you happiness does not know where to shop. Your stand concerning this statement will depend on your level of income and the importance of money for your well-being. Since some might argue that having money is not everything, it then raises the question of what does it take to be happy? What is the government's role in this endeavor? And how can happiness be sustained? This study presents some principles about money and happiness. It suggests that while being rich might not necessarily be the main or only path to happiness, having a higher income will guarantee access to homes in safer neighborhoods, better healthcare, and nutrition, provide access to higher education for your family, give you a sense of fulfillment, work satisfaction, and more leisure time. Note that how you spend, save, and think about money shapes how much joy you get from it. Above all, happiness is a subjective experience. It is about the satisfaction you derive from the way your life is going. Happiness is about personal freedom to make important life choices, such as shaping your life the way you want it.
CODE-SWITCHING IN MULTILINGUAL ROVING PEOPLE’S CONVERSATIONS
Authors: Bramantoko Beda , Ardi Priyatno
Published: February 2023

This paper aims at analyzing the types of code-switching in the conversation of multilingual roving people, who move from one country to another country. The participants of this qualitative research were five multilingual roving people. Audio recordings and interviews were used to collect the data. The results reveal that the types of code-switching employed by the multilingual roving people were intra-sentential switching, inter-sentential switching, situational switching, and metaphorical switching. The researchers also found that the factors influencing the multilingual roving people in switching the codes included the speaker, interlocutors, changes in the situation because of the third person, and changes in the topic. The researchers concluded that the type of code-switching that appeared the most was intra-sentential switching and the most influencing factor why the participants did code-switching was the interlocutors.
Ciencia y saberes en la edad moderna: un espejo para reflexionar sobre el panorama historiográfico en la era de la mundialización
Authors: Romano Antonella
Published: February 2023

Se presenta un minucioso recorrido de la recepción de las obras, escritas en el mundo anglófono, sobre la historia de las ciencias, puestas en relación con la propia producción de tradición francesa. Su artículo, que en un momento nos pone al día de la producción francesa sobre el tema, en su segunda parte es un diálogo con Simon Schaffer, quien, en sus últimos artículos, ha venido investigando sobre las conexiones, recepción y aportaciones de las culturas "colonizadas" (el subcontinente asiático, India, principalmente) a la ciencia europea. En suma, podríamos decir, que desde Europa hace una dura crítica al eurocentrismo que postula haber construido la ciencia de la modernidad (siglos XVII y XVIII) aisladamente. Al lado de la obra de Schaffer, la autora (especialista en historia de las ciencias en la modernidad temprana y las relaciones con el mundo chino) devela el intercambio con estos mundos y las abre a la perspectiva mundial.
Design for Health and Well-Being. The Housing Needs of Future Seniors
Authors: Gawlak Agata
Published: February 2023

This paper presents the results of the research made on a sample of 2,000 of young respondents surveyed in the scope of their housing needs, including their expectations as to the place of residence, form of residence and size of their living space. The methodology assumed for the presentation of results of quantitative variables included such statistical measures as the arithmetic mean, standard deviation or quantity. Whereas, the quantitative variables have been presented via quantity and percentages. To assess the percentage difference between the analysed parameters, the author has used Pearson's chi-squared test, chi-squared test and others. Comparative analyses drawn up on the basis of the data show the tendencies in the housing preferences among young people, both in the short and long time perspective. The research was intended to diagnose the housing needs of young people and to juxtapose their expectations with the tendencies. It can effectively help architects create solutions that properly meet the factual needs of the residents as well as improve the quality of designed housing for the future senior residents.
The Criterion of Access to the Sky View in Residential Environment Architectural Designing
Authors: Pelczarski Zdzisław
Published: February 2023

Standards specifying the requirements for daylight in shaping the interiors of residential buildings do not cover issues related to visual perception and the role of light as a carrier of images of the outside world. The general recommendations in this regard, given in the 2018 European Lighting Standard can hardly be considered an unambiguous, precise normative regulation. According to the author, the inalienable element of the view through the window is the image of the sky, which is the basic reference in human perception of the space of the external environment. The aim of the research is to identify the main determinants for ensuring access to the sky view from apartments in housing estates with high intensity of development. The analyzes are based on the author's simple graphical research methods capable of simulating the visual perception of images of the external environment space through a window. The key parameter in the analyzes is the vertical viewing angle, called the sky view entry angle. Its minimum value set at 7.5°C is of fundamental importance in shaping the geometric relations between neighbouring buildings. The research results oblige, in the context of the paradigm of sustainable housing development, to recognize the criterion of access to the sky view from residential interiors as a mandatory design guideline.
Hubungan Status Gizi dengan Power Atlet Tapak Suci Universitas Muhammadiyah Jakarta
Authors: Saraswati Nadya , Kusumaningati Walliyana
Published: February 2023

Latar belakang: Tapak Suci merupakan cabang olahraga yang menjadi warisan budaya Indonesia. Olahraga ini dipertandingkan berdasarkan kelas dengan kategori berat badan. Beberapa atlet mentargetkan lawan yang mempunyai berat badan lebih kecil agar dapat menggunakan power lebih baik dengan memanfaatkan masa otot yang lebih besar. Salah satu keberhasilan olahraga ini adalah power yang diperlukan oleh atlet tapak suci untuk melakukan pukulan, tendangan, tangkisan dan bantingan. Power dikaitkan dengan status yang baik untuk menjaga performance pada proses latihan maupun pertandingan. Tujuan: Penelitian ini bertujuan untuk melihat hubungan antara status gizi dengan power atlet tapak suci UMJ. Metode: Penelitian ini merupakan penelitian kuantitatif dengan desain cross-sectional, Responden penelitian adalah Atlet Tapak Suci UMJ sebesar 58 responden yang dipilih dengan teknik random sampling. Penelitian dilakukan pada bulan April-Juni tahun 2022. Status gizi dinilai dengan pengukuran antropometri dengan Indeks Massa Tubuh (IMT), sedangkan power dinilai menggunakan pengukuran vertical jump. Data dianalisis secara bivariat mengggunakan uji chi-square dengan SPSS. Hasil: Sebagian besar atlet memiliki kategori status gizi normal (69%), dan power berkategori baik (62,1%). Hasil uji chi square menyatakan status gizi secara bermakna (p=0,032) berhubungan dengan power atlet. Simpulan: Terdapat hubungan antara status gizi dan power atlet tapak suci UMJ dengan power atlet tapak suci UMJ.
Aktivitas Antioksidan dan Kandungan Gizi Biskuit Rempah dengan Penambahan Sari Kurma Sebagai Pangan Fungsional untuk Imunitas Tubuh
Authors: Yuliarti Nurul Azizah Tri , Dainy Nunung Cipta , Yunieswati Wilda
Published: February 2023

Latar belakang: Imunitas tubuh yang kuat dibutuhkan agar terhindar dari paparan virus, bakteri dan parasit. Asupan gizi yang dapat meningkatkan imunitas tubuh yaitu antioksidan. Rempah dan kurma merupakan salah satu pangan sumber antioksidan. Tujuan: Menganalisis aktivitas antioksidan dan kandungan gizi biskuit rempah dengan penambahan sari kurma untuk imunitas tubuh. Metode: Penelitian ini merupakan studi eksperimental menggunakan Rancangan Acak Lengkap (RAL). Terdapat lima formula biskuit rempah dengan perlakuan yang berbeda yaitu F0 (tanpa sari kurma), F1 (20 gram sari kurma), F2 (30 gram sari kurma), F3 (40 gram sari kurma), F4 (50 gram sari kurma) yang diujikan kepada 30 panelis semi terlatih. Analisis data menggunakan uji Anova dan uji lanjut Duncan. Analisis aktivitas antioksidan menggunakan metode AEAC, dan analisis kandungan gizi menggunakan uji proksimat. Hasil: Hasil penelitian menunjukkan bahwa F2 dengan penambahan sari kurma 30gram merupakan formula yang paling disukai panelis dengan aktivitas antioksidan (IC50) 513,8 ppm, kandungan energi 478 kkal/100 g, kadar protein 4,69%, dan kadar lemak total 25,09%. Simpulan: Biskuit rempah terpilih dengan penambahan sari kurma 30 gram, memiliki aktivitas antioksidan sangat lemah (513,8 ppm) dengan kadar protein, kadar air, dan kadar abu yang belum memenuhi SNI biskuit.
Analisis Nilai Kemurnian DNA Menggunakan Nano Fotometer pada Rasio 260/230 yang Diisolasi dari Produk Nugget
Authors: Sophian Alfi , Yustina Yustina
Published: February 2023

Latar belakang: Parameter nilai kemurnian yang dianalisis dari panjang gelombang dengan rasio 260/230 merupakan parameter validasi sekunder yang digunakan untuk melakukan analisis mutu DNA hasil isolasi pada produk nugget ayam. Tujuan: Tujuan dari dilakukannya penelitian ini adalah untuk memberi informasi tentang nilai analisis kemurnian DNA hasil isolasi yang dibaca pada rasio 260/230.  Dengan diperolehnya informasi dari hasil penelitian ini diharapkan dapat memberi manfaat terhadap penelitian sejenis yang relevan. Metode: Metode yang digunakan dalam penelitian ini adalah metode isolasi DNA menggunakan teknik spin kolom, kemudian DNA hasil isolasi yang diperoleh dibaca menggunakan nano fotometer pada rasio 260/230. Hasil: Hasil pengukuran kemudian dihitung nilai rata-rata dan standar deviasinya.  Berdasarkan hasil pengukuran DNA hasil isolasi diperoleh hasil kemurnian yang dibaca pada rasio A260/A230 berada pada kisaran 1,98 – 2,10, dengan nilai rata-rata berada pada nilai 2,043. Simpulan: Berdasarkan hasil tersebut maka dapat disimpulkan bahwa DNA hasil isolasi yang diperoleh menunjukkan kualitas DNA yang baik berdasarkan syarat mutu nilai kemurnian DNA pada rasio 260/230 yaitu pada kisaran 2,0 – 2,2.
Karakteristik Lansia dan Kejadian Depresi di Panti Pelayanan Sosial Lanjut Usia (PPSLU) Sodagaran Banyumas
Authors: Septianawati Paramita , Mustikawati Irma Finurina , Kusuma Inggar Ratna , Maulana Andi Muh.
Published: February 2023

Latar belakang: Peningkatan jumlah penduduk lansia diperkirakan akan menjadi masalah Kesehatan di Indonesia. Salah satu masalah pada lansia adalah depresi. Penelitian ini diperlukan untuk menginvestigasi karakteristik lansia yang mengalami depresi. Tujuan: Melihat gambaran hubungan karakteristik lansia terhadap kejadian depresi di Panti Pelayanan Sosial Lanjut Usia (PPSLU) Sodagaran Banyumas. Metode: penelitian ini adalah cross-sectional dengan responden jumlah 42 orang dengan mengisi data pribadi, kuesioner Geriatric Depression Scale (GDS) dan mengikuti pemeriksaan fisik. Hasil: Hasil penelitian didapatkan 33 responden lansia tidak mengalami depresi serta 9 orang lansia mengalami depresi ringan berdasarkan GDS. Dalam analisis bivariat ditemukan adanya hubungan antara jenis kelamin (p=0,046), riwayat sekolah (p=0,032) dan riwayat bekerja (p=0,017) pada lansia yang mengalami depresi ringan dan tidak depresi. Selain itu tidak ada hubungan signifikan (p>0,05) antara usia, riwayat menikah, lama tinggal, tekanan darah, Body Mass Index (BMI) dan lingkar pinggang pada lansia yang tidak depresi dengan lansia yang depresi. Simpulan: Diperoleh laki-laki yang memiliki riwayat pernah sekolah dan riwayat pernah bekerja lebih cenderung mengalami depresi ringan.
Pengaruh Ekstrak Etanol Daun Kersen (Muntingia calabura L.) terhadap Peningkatan Kadar Insulin Tikus Putih Model Diabetes Melitus Tipe 2 setelah Induksi STZ-NA
Authors: Azhar Religia , Romdhoni Muhammad Fadhol , Karita Dewi , Bahar Yenni
Published: February 2023

Latar Belakang: Diabetes melitus tipe 2 adalah kelainan metabolisme yang terjadi secara kronis, ditandai dengan kenaikan gula darah akibat penurunan sekresi dan fungsi insulin oleh sel beta pankreas atau terjadinya resistensi insulin. Diabetes Melitus (DM) dapat diobati secara medis dengan obat antidiabetik oral atau suntikan insulin. Namun karena mahalnya biaya, perawatan medis terkadang sulit dilakukan sehingga pengobatan DM juga dapat diatasi menggunakan tanaman obat berkhasiat, salah satunya daun kersen yang mengandung senyawa antidiabetik. Tujuan: mengetahui pengaruh ekstrak ethanol daun kersen terhadap kadar insulin pada tikus putih galur wistar penderita DM tipe 2 yang diinduksi STZ-NA. Metode: Penelitian ini berjenis eksperimental laboratorium dengan teknik simple random sampling, post-test only control group design. Analisis data menggunakan uji statistik parametrik One Way ANOVA dilanjutkan dengan post hoc LSD. Hasil: Rata-rata kadar insulin tertinggi adalah KI (0,3 mg/gBB) yaitu 7,43925 mIU/L. Uji one-way ANOVA menunjukkan perbedaan yang signifikan (p<0,05). Uji post hoc LSD menunjukkan perbedaan bermakna antara kelompok perlakuan dan kontrol negatif (p<0,05). Simpulan: Terdapat pengaruh ekstrak ethanoldaun kersen terhadap peningkatan kadar insulin pada tikus putih galur wistar penderita DM Tipe 2 yang diinduksi STZ-NA dengan dosis yang paling efektif 0,3 mg/gBB.
Karakteristik Pasien Osteoartritis Genu di Poli Rehabilitasi Medik RS Setia Mitra Tahun 2020
Authors: Ghassani Fildzah Siti , Idris Ferial Hadipoetro
Published: February 2023

Latar belakang: Osteoartritis (OA) merupakan penyakit kronis yang ditandai dengan kerusakan tulang rawan pada persendian dan menimbulkan kekakuan, nyeri, dan gangguan gerakan. OA merupakan bentuk paling umum dari arthritis, terdapat peningkatan YLD (Years with Disability) sebesar 9,6%. Prevalensi OA di Indonesia pada pria sebesar 5% dan pada wanita 12,7% berbanding lurus dengan pertambahan usia, yaitu  sebesar 5% pada individu berusia kurang dari 40 tahun, 30% pada usia 40–60 tahun, dan 65% pada usia di atas 60 tahun. Rumah sakit memerlukan data karakteristik pasien OA agar lebih siap dalam melayani   pasien OA. Tujuan: Diketahuinya karakteristik pasien OA genu di Poli Rehabilitasi Medik Rumah Sakit Setia Mitra  pada tahun  2020. Metode: Studi Deskriptif dengan pendekatan cross-sectional melalui total sampling ini melibatkan 38 data pasien dari rekam medik dengan analisis univariat. Hasil: Karakteristik pasien usia >60 tahun  60,5 %, jenis kelamin perempuan 86,8%, berat badan 54-60 kg sebesar 39,5%, dengan pekerjaan pensiunan sebesar 57,9%. Simpulan: Karakteristik pasien OA genu  di Poli Rehabilitasi Medik Rumah Sakit Setia Mitra  pada tahun  2020 berdasarkan usia terbanyak adalah >60 tahun (60,5 %), jenis kelamin  terbanyak  perempuan (86,8%), berat badan 54-60 kg (39,5%), dan pekerjaan terbanyak merupakan pensiunan (57,9%).
Kehamilan Tidak Diinginkan di Usia Remaja
Authors: Fauziah Putri Salmah , Hamidah Hamidah , Subiyatin Aning
Published: February 2023

Latar belakang: Kehamilan tidak diinginkan sudah banyak dialami oleh remaja perempuan akibat aktivitas seksual pranikah. Kehamilan tidak diinginkan pada remaja dapat menimbulkan tingginya aborsi yang dapat memicu pada peningkatan angka kematian ibu di Indonesia. Tujuan: Penelitian ini bertujuan untuk mendapatkan informasi secara mendalam tentang kehamilan tidak diinginkan pada remaja di Yayasan Rumah Tumbuh Harapan Kota Bandung. Metode: Penelitian ini menggunakan metode kualitatif dengan pendekatan fenomenologi. Pengambilan sampel dengan purposive sampling. Pengumpulan data dilakukan melalui Focus Group Discussion (FGD) dengan lima remaja, kemudian dilakukan wawancara mendalam dengan salah satu pengurus Yayasan Rumah Tumbuh Harapan dan studi dokumentasi sebagai bentuk triangulasi. Hasil FGD dan wawancara dituliskan dalam bentuk transkrip verbatim lalu dilakukan analisis tematik dan koding, lalu hasil studi dokumentasi dalam bentuk data demografi dari masing-masing remaja, profil Yayasan dan foto kegiatan. Selanjutnya dilakukan reduksi dan penyajian data. Hasil: Didapatkan empat tema yang mempengaruhi terjadinya kehamilan tidak diinginkan pada remaja yaitu pengetahuan, kekerasan dalam pacaran, pergaulan bebas dan peran keluarga, dimana kurangnya pengetahuan, kurangnya peran keluarga serta pergaulan yang bebas membuat remaja berperilaku diluar batas yang mengakibatkan kehamilan tidak diinginkan. Simpulan: Kehamilan tidak diinginkan pada remaja disebabkan kurangnya peran orang tua dalam pola asuh untuk memberikan pendidikan, perhatian dan pengawasan yang baik pada anaknya agar tidak berperilaku diluar kendali orang tuanya.
Efektivitas Lembar Balik Terhadap Pengetahuan dan Sikap Ibu Hamil Tentang Sunat Perempuan Tahun 2022
Authors: Winancy Winancy , Mustofani Ramadhani , Jehanara Jehanara
Published: February 2023

Latar belakang: Persentase anak perempuan usia 0-11 tahun di Indonesia tahun 2013 yang pernah disunat sebesar 51,2 %, sunat perempuan tidak memiliki manfaat kesehatan dan dapat membahayakan menurut World Health Organization. Praktik sunat perempuan masih dilakukan di wilayah Jatimakmur Bekasi dengan kejadian 4 dari 5 ibu anaknya dilakukan sunat perempuan. Tujuan: Untuk mengetahui efektivitas media lembar balik terhadap pengetahuan dan sikap ibu hamil tentang sunat perempuan. Metode: Penelitian ini menggunakan desain kuantitatif pre eksperimental dengan rancangan one group pretest-posttest. Teknik pengambilan sampel menggunakan accidental sampling di posyandu wilayah Jatimakmur. Jumlah sampel penelitian sebanyak 55 responden. Analisis data menggunakan analisis univariat dan analisis bivariat (Wilcoxon Test). Hasil: Terdapat peningkatan pengetahuan dan sikap ibu hamil sebelum dan setelah diberikan edukasi tentang sunat perempuan menggunakan media lembar balik, nilai rerata skor pengetahuan sebelum diberikan intervensi sebesar 57,80 dan setelah intervensi sebesar 77,93. Rerata skor sikap sebelum intervensi sebesar 54,29 dan setelah intervensi sebesar 70,29. Nilai P value=0.0001 (p<0,05). Simpulan: Media lembar balik dapat digunakan sebagai media pendidikan kesehatan tentang sunat perempuan dan dapat dilakukan penelitian lebih lanjut terkait faktor yang mempengaruhi serta variabel lainya.
Analisis Tindakan untuk Ibu Menyusui Terkait Sikap dan Social Distancing pada Masa Pandemi COVID-19
Authors: Sari Fitria , Aprillia Yuna Trisuci , Mawarni Endang Siti
Published: February 2023

Latar belakang: Pandemi COVID-19 mengakibatkan kekhawatiran penularan virus oleh ibu kepada bayinya, sedangkan menyusui memberikan banyak manfaat bagi bayi. Virus tersebut belum terdeteksi pada ASI ibu yang positif COVID-19. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan antara sikap, social distancing dengan tindakan ibu menyusui selama pandemi COVID-19. Metode: Penelitian ini menggunakan desain cross sectional dan teknik sampling aksidental. Pengumpulan data dilakukan dengan mengisi kuesioner gform. Total subjek sebanyak 152 responden yang merupakan ibu menyusui di Indonesia. Analisis data menggunakan univariat dan bivariat dengan kai kuadrat. Hasil: Analisis univariat,menunjukkan hasil yaitu responden yang tindakan menyusuinnya baik sebesar 90 (59,2%), responden yang sikapnya baik sebesar 91 (59.9%) dan responden yang tidak melakukan social distancing dengan bayinya sebesar 94 (61,8%), pada analisa bivariat menunjukkan bahwa variabel sikap tidak berhubungan dengan tindakan ibu menyusui (p=0,586). Sedangkan variabel social distancing ada hubungan dengan tindakan untuk ibu menyusui (p=0,006). Simpulan: Ada hubungan yang signifikan antara jaga jarak (social distancing) dengan tindakan bagi ibu menyusui pada masa pandemi COVID-19, sedangkan sikap tidak ada hubungan yang signifikan.
Controllable Synthesis of Heterofunctionalized Polyethers via an Intramolecular Phosphonium Bisborane Lewis Pair Catalysed Immortal Ring Opening Polymerization
Authors: Wang Xiaowu , Shi Minmin , Hui Jiwen , Kou Xinhui , Wang Jiansen , Zhong Linrong , Li Zhibo
Published: February 2023

A metal-free phosphonium bisborane Lewis pair (PBB-Br) was demonstrated to realize the immortal ring-opening polymerization (iROP) of propylene oxide (PO) under mild conditions with alcohols as chain transfer agents (CTAs). Narrow dispersed poly(propylene oxide) (PPO) with controllable molecular weights predicted from [PO]0/([PBB-Br]0+[CTAs]0) molar ratio were obtained in quantitative conversion. These indicated the iROP feature of PBB-Br-catalysed PO polymerization. The rapid, reversible and quantitative chain transfer assured the iROP characterisitc for intramolecular Lewis pair catalyst PBB-Br, so various well-defined heterofunctionalized PPOs were easily produced. All the α,ω-difunctionalized PPOs were carefully characterized. Density functional theory (DFT) calculations reveal the chain transfer to CTA process is almost barrierless (0.8 kcal. mol-1) and thermodynamically favorable as compared to chain propagation. Moreover, PPO-based block copolyethers were easily obtained in one-pot using epoxide mixtures. This research demonstrated that the delicately designed intramolecular synergistic Lewis pair offered a powerful and controllable method to prepare various heterofunctionalized PPO samples with high values.
Self-assembly of insulin-derived chimeric peptides into two-component amyloid fibrils: the role of Coulombic interactions
Authors: Fortunka Mateusz , Dec Robert , Puławski Wojciech , Guza Marcin , Dzwolak Wojciech
Published: February 2023

Canonical amyloid fibrils are composed of covalently identical polypeptide chains. Here, we employ kinetic assays, atomic force microscopy (AFM), infrared spectroscopy, circular dichroism (CD), and molecular dynamics (MD) to study fibrillization patterns of two chimeric peptides, ACC1-13E8 and ACC1-13K8, in which potent amyloidogenic stretch derived from the N-terminal segment of insulin A-chain (ACC1-13) is coupled to octaglutamate or octalysine segments, respectively. While the large electric charges on monomers of either peptide prevent aggregation at neutral pH, stoichiometric mixing of ACC1-13E8 and ACC1-13K8 triggers rapid self-assembly of two-component fibrils driven by favorable Coulombic interactions. The role of low-symmetry non-polar ACC1-13 pilot sequence is crucial in enforcing the amyloidal parallel -sheet motif as self-assembly of free poly-E and poly-K chains under similar conditions results in amorphous antiparallel -sheet conformation. Interestingly, the pathway to highly ordered fibrils is accessible to ACC1-13E8 also when paired with non-polypeptide polycationic amines such as branched poly-ethylenimine, PEI, instead of ACC1-13K8. Remarkably, such synthetic polycations are more effective in triggering fibrillization of ACC1-13E8 than poly-K (or poly-E in the case of ACC1-13K8). High conformational flexibility of these polyamines makes up for the apparent mismatch in periodicity of charged groups. The results are discussed in the context of mechanisms of heterogenous disease-related amyloidogenesis.
CRIMES AGAINST THE CIVIL POPULATION IN THE TERRITORY OF THE OCCUPIED REGIONS OF THE KFSSR DURING THE GREAT PATRIOTIC WAR (BY THE EXAMPLE OF KINDASOVSKY CONCENTRATION CAMP)
Authors: Зеленская Юлия Николаевна
Published: February 2023

В статье на основе рассекреченных документов архива Управления Федеральной службы безопасности Российской Федерации по Республике Карелия и Национального архива Республики Карелия представлена картина лагерного быта заключенных Киндасовского концентрационного лагеря тюремного типа. Лагерь находился на территории Пряжинского района Карело-Финской ССР в годы Великой Отечественной войны и предназначался для «неблагонадежных» советских граждан. На основании протоколов допросов и воспоминаний узников удалось установить распорядок дня, условия труда и отдыха, рацион питания, отношение руководства и лагерной охраны к заключенным. Киндасовский лагерь отличался особенно жестоким режимом. Лагерная администрация имела всеобъемлющую власть и не боялась наказания за совершенные преступления против личности. На примере лагеря в Киндасово в статье показаны проявления преступной политики администрации Военного Управления Восточной Карелии на территории оккупированных районов КФССР.

Based on declassified sources of the archive of the Federal Security Service of the Russian Federation for the Republic of Karelia and the National Archive of the Republic of Karelia, the article presents a picture of the camp inmates of the Kindasovskoye prison-type concentration camp. The camp was located on the territory of the Pryazhinsky district of the KFSSR during the Great Patriotic War and was intended for «unreliable» Soviet citizens. Based on the protocols of interrogations and memoirs of prisoners, it was possible to establish the daily routine, working and rest conditions, diet, attitude of the leadership and camp guards towards prisoners. Kindasovskogo camp was distinguished by a particularly cruel regime. The camp administration had allencompassing power and was not afraid of punishment for committed crimes against the person. The camp in Kindasovo is the example for the article to show the manifestations of the criminal policy of the administration of the Military Directorate of Eastern Karelia in the territory of the occupied regions of the KFSSR.
ACTIVE CHURCHES OF THE KALININ DIOCESE, 1948-1949 (ACCORDING TO THE REPORTS OF THE DEANS AND THE ADMINISTRATOR OF THE DIOCESE)
Authors: Леонтьева Татьяна Геннадьевна
Published: February 2023

В публикации представлены предварительные результаты исследования в рамках проекта «Церковная жизнь в советском обществе в 1940-1950-гг.: религиозные практики населения в Калининской области в воспоминаниях “детей войны”». Публикация подготовлена на основании анализа и систематизации материалов, отложившихся в Архивном отделе научной библиотеки Тверской епархии (Тверская митрополия). Выявлена численность действующих и закрытых православных храмов, служащего духовенства и нештатных сотрудников (псаломщиков, регентов, певчих, сторожей, уборщиц). Данные из отчетов управляющего епархией епископа Арсения, благочинных церковных округов и приходских священников, писем уполномоченного Совета по делам Русской православной церкви при Совете министров СССР по Калининской области характеризуют негативные изменения во внутрицерковной жизни, которые просматриваются с 1948-1949 гг.

The publication presents the preliminary results in the project: «Church life in Soviet society in the 1940s-1950s: religious practices of the population in the Kalinin region in the memoirs of “war children”». The publication based on the analysis of the systemized materials deposited in the Archival Department of the Ecclesiastic Library of the Tver Diocese (Tver Metropolia). So there are revealed the whole number of active and closed orthodox churches, serving clergy and non-staff employees (psalmists, regents, singers, watchmen, cleaners). Data from reports of Bishop Arseny, the administrator of the diocese, deanery of church districts and parish priests, letters of the authorized Council for the Affairs of the Russian Orthodox Church under the Council of Ministers of the USSR in the Kalinin region characterize negative changes in church life, which are viewed from 1948-1949.
WEDDING RITES AT THE RUSSIAN NOBLES IN THE 18TH - THE MIDDLE OF THE 19TH CENTURIES: RITUALS AND PRACTICES
Authors: Белова Анна Валерьевна
Published: February 2023

Статья посвящена свадебной обрядности у российских дворян как традиционного сообщества в XVIII - середине XIX в. На основе исследования неопубликованных архивных документов из личных фондов дворянских родов и опубликованных автодокументальных свидетельств реконструируются основные элементы свадебного обряда, включая длительную процедуру, предшествовавшую непосредственному заключению брака во время церковного таинства венчания. В статье анализируются культурно-антропологический характер и функциональное предназначение социального ритуала, выясняется сопряженность коллективных практик и индивидуальных предпочтений. Особое внимание уделяется проблемам дворянского этоса и воспроизводству этических норм, зафиксированных в эгодокументах, гендерным аспектам обрядовых действий. В заключении делается вывод о том, что свадебные ритуалы относятся к сфере мужских престижных взаимодействий и практикам экспрессивного порядка, в которых женская роль сводится к инструментальному посредничеству в скреплении межродовых связей.

The article is devoted to the wedding rites at the Russian nobles as a traditional community in the 18th - the middle of the 19th centuries. Based on the study of unpublished archival documents from the personal funds of noble families and published autodocumentary evidence, the author reconstructs the main elements of the wedding ceremony, including the lengthy procedure that preceded the direct marriage during the church sacrament of the wedding. The article analyzes the cultural and anthropological nature and functional purpose of the social ritual, reveals the conjugation of collective practices and individual preferences. Particular attention is paid to the problems of the noble ethos and the reproduction of ethical norms recorded in egodocuments, as well as the gender aspects of ritual actions. In conclusion the author claims that wedding rituals belong to the sphere of men’s prestigious interactions and practices of an expressive order, in which the women’s role is reduced to instrumental mediation in strengthening of the interclan ties.