bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Quality of Life Related to Health and Vision in a Group of Patients with Diabetic Retinopathy
Authors: Roberts-Martínez Aguirre Ian , Rodríguez-Fernández Paula , González-Santos Josefa , Aguirre-Juaristi Nerea , Alonso-Santander Nuria , Mielgo-Ayuso Juan , González-Bernal Jerónimo J.
Published: January 2022

(1) Background: Visual impairment of people with diabetic retinopathy (DR) and its high impact on different dimensions of their lives can cause a significant deterioration in the quality of life. The aim of this study was to examine the association and relationship between quality of life related to vision and the relevant clinical and sociodemographic variables in a group of patients with DR in Spain. (2) Methods: A descriptive cross-sectional study was conducted in all patients with DR over 18 years under follow-up in the Retina Service of the University Hospital of Burgos (HUBU), recruited during the months of January and February 2020. The main study variable was quality of life related to health and vision, obtained using the National Eye Institute Visual Function Questionnaire 25 (NEI-VFQ-25). (3) Results: In total 87 participants made up the sample, and significant differences were found in the NEI-VFQ-25 according to gender, type of diabetes, episodes of decompensated diabetes and high blood pressure (HBP) (p < 0.05). Best-corrected visual acuity (BCVA) was also correlated with the NEI-VFQ-25 (p < 0.05). (4) Conclusions: These data could facilitate the design of action protocols focused on the well-being of the patient, in addition to considering the clinical characteristics. Further studies are needed to help understand the causal relationship between variables and that includes a wider variety of factors.
Poor Diet, Long Sleep, and Lack of Physical Activity Are Associated with Inflammation among Non-Demented Community-Dwelling Elderly
Authors: Basta Maria , Belogianni Christina , Yannakoulia Mary , Zaganas Ioannis , Panagiotakis Symeon , Simos Panagiotis , Vgontzas Alexandros N.
Published: January 2022

Inflammation in elderly is associated with physical and cognitive morbidity and mortality. We aimed to explore the association of modifiable lifestyle parameters with inflammation among non-demented, community-dwelling elderly. A sub-sample of 117 patients with mild cognitive impairment (MCI, n = 63) and cognitively non-impaired controls (CNI, n = 54) were recruited from a large, population-based cohort in Crete, Greece, of 3140 elders (>60 years old). All participants underwent assessment of medical history/physical examination, extensive neuropsychiatric/neuropsychological evaluation, diet, three-day 24-h actigraphy, subjective sleep, physical activity, and measurement of IL-6 and TNFα plasma levels. Associations between inflammatory markers and diet, objective sleep duration, subjective sleep quality, and lack of physical activity were assessed using multivariate models. Regression analyses in the total group revealed significant associations between TNF-α and low vegetable consumption (p = 0.003), and marginally with objective long nighttime sleep duration (p = 0.04). In addition, IL-6 was associated with low vegetable consumption (p = 0.001) and lack of physical activity (p = 0.001). Poor diet and lack of physical activity appear to be modifiable risk factors of inflammation, whereas long sleep appears to be a marker of increased inflammatory response in elderly. Our findings may have clinical implications given the association of inflammatory response with morbidity, including cognitive decline, and mortality in elderly.
Sleep Medication in Older Adults: Identifying the Need for Support by a Community Pharmacist
Authors: Masse Morgane , Henry Héloïse , Cuvelier Elodie , Pinçon Claire , Pavy Margot , Beeuwsaert Audrey , Barthélémy Christine , Cuny Damien , Gautier Sophie , Kambia Nicolas , Lefebvre Jean-Marc , Mascaut Daniel , Mitoumba Fabrice , Puisieux François , Standaert Annie , Wierre Patrick , Beuscart Jean-Baptiste , Roche Jean , Décaudin Bertrand
Published: January 2022

Many older adults take benzodiazepines and sedative-hypnotics for the treatment of sleep disorders. With a view to considering the possible discontinuation of hypnotics, the objectives of the present study were to describe bedtime habits and sleep patterns in older adults and to identify the sleep medications taken. An expert group developed a structured interview guide for assessing the patients’ bedtime habits, sleep patterns, and medications. During an internship in a community pharmacy, 103 sixth-year pharmacy students conducted around 10 interviews each with older adults (aged 65 or over) complaining of sleep disorders and taking at least one of the following medications: benzodiazepines, benzodiazepine derivatives (“Z-drugs”), antihistamines, and melatonin. A prospective, observational study was carried out from 4 January to 30 June 2016. The pharmacy students performed 960 interviews (with 330 men and 630 women; mean ± standard deviation age: 75.1 ± 8.8). The most commonly taken hypnotics were the Z-drugs zolpidem (n = 465, 48%) and zopiclone (n = 259, 27%). The vast majority of patients (n = 768, 80%) had only ever taken a single hypnotic medication. The median [interquartile range] prescription duration was 120 (48–180) months. About 75% (n = 696) of the patients had at least 1 poor sleep habit, and over 41% (n = 374) had 2 or more poor sleep habits. A total of 742 of the patients (77%) reported getting up at night—mainly due to nycturia (n = 481, 51%). Further, 330 of the patients (35%) stated that they were keen to discontinue their medication, of which 96 (29%) authorized the pharmacist to contact their family physician and discuss discontinuation. In France, pharmacy students and supervising community pharmacists can identify problems related to sleep disorders by asking simple questions about the patient’s sleep patterns. Together with family physicians, community pharmacists can encourage patients to discuss their hypnotic medications.
Using Heat as a Tracer to Detect the Development of the Recharge Bulb in Managed Aquifer Recharge Schemes
Authors: Caligaris Esteban , Agostini Margherita , Rossetto Rudy
Published: January 2022

Managed Aquifer Recharge (MAR), the intentional recharge of aquifers, has surged worldwide in the last 60 years as one of the options to preserve and increase water resources availability. However, estimating the extent of the area impacted by the recharge operations is not an obvious task. In this descriptive study, we monitored the spatiotemporal variation of the groundwater temperature in a phreatic aquifer before and during MAR operations, for 15 days, at the LIFE REWAT pilot infiltration basin using surface water as recharge source. The study was carried out in the winter season, taking advantage of the existing marked difference in temperature between the surface water (cold, between 8 and 13 °C, and in quasi-equilibrium with the air temperature) and the groundwater temperature, ranging between 10 and 18 °C. This difference in heat carried by groundwater was then used as a tracer. Results show that in the experiment the cold infiltrated surface water moved through the aquifer, allowing us to identify the development and extension in two dimensions of the recharge plume resulting from the MAR infiltration basin operations. Forced convection is the dominant heat transport mechanism. Further data, to be gathered at high frequency, and modeling analyses using the heat distribution at different depths are needed to identify the evolution of the recharge bulb in the three-dimensional space.
Removal of Arsenic in Groundwater Using Fe(III) Oxyhydroxide Coated Sand: A Case Study in Mekong Delta, Vietnam
Authors: Kim Lavane , Thanh Nguyen Truong , Toan Pham Van , Minh Huynh Vuong Thu , Kumar Pankaj
Published: January 2022

Because of its threat to the quality of freshwater resources and human health, arsenic (As) pollution is important to scientific communities and policymakers around the world. The Mekong Delta, Vietnam, is one hotspot of As pollution. Its risk assessment of different environmental components has been well documented; however, very few studies focus on As removal techniques. Considering this information gap, this study aimed to investigate the performance of an innovative and low-cost treatment system using Fe(III)-oxyhydroxide (FeOOH) coated sand to remove As(III) from aqueous solution. Batch and column experiments were conducted at a laboratory scale in order to study removal kinetics and efficiency. Experimental results indicated that the adsorption isotherm of As(III) on FeOOH coated sand using Langmuir and Freundlich models have high regression factors of 0.987 and 0.991, respectively. The batch adsorption experiment revealed that contact time was approximately 8 h for rough saturation (kinetic test). The concentration of As(III) in effluents at flow rates of 0.6 L/h, 0.9 L/h, and 1.8 L/h ranged from 1.1 µg/L to 1.7 µg/L. Results from this study indicated that FeOOH coated sand columns were effective in removing As(III) from water, with a removal efficiency of 99.1%. Ultimately, FeOOH coated sand filtration could be a potential treatment system to reduce As(III) in the domestic water supply in remote areas of the Vietnamese Mekong Delta.
Does Overseas Investment Raise Corporate Environmental Protection? Evidence from Chinese A-List Companies
Authors: Wang Quan-Jing , Shen Qiong , Geng Yong , Li Dan-Yang
Published: January 2022

This paper uses the relevant data of China’s listed companies from 2010 to 2018 to test the impact of overseas investment on corporate environmental protection and further examines whether the heterogeneity of the company and the heterogeneity of the host country changes this effect. The research results show that the environmental protection of overseas investment companies is significantly higher than that of other companies. The impact of overseas investment on corporate environmental protection is dynamic, and it only helps improve corporate environmental protection after three years of investment. This article is conducive to causally identifying the logical relationship between overseas investment and corporate environmental protection. The policy significance is that the government can rationally guide companies to invest abroad, and oversea investment will help enhance corporate environmental protection.
External Exposure to BTEX, Internal Biomarker Response, and Health Risk Assessment of Nonoccupational Populations near a Coking Plant in Southwest China
Authors: Qin Ning , Zhu Yuanyuan , Zhong Yan , Tian Jing , Li Jihua , Chen Laiguo , Fan Ruifang , Wei Fusheng
Published: January 2022

Benzene, toluene, ethylbenzene and xylene isomers (BTEX) have raised increasing concern due to their adverse effects on human health. In this study, a coking factory and four communities nearby were selected as the research area. Atmospheric BTEX samples were collected and determined by a preconcentrator GC–MS method. Four biomarkers in the morning urine samples of 174 participants from the communities were measured by LC–MS. The health risks of BTEX exposure via inhalation were estimated. This study aimed to investigate the influence of external BTEX exposure on the internal biomarker levels and quantitatively evaluate the health risk of populations near the coking industry. The results showed that the average total BTEX concentration in residential area was 7.17 ± 7.24 μg m−3. Trans,trans-muconic acid (T,T-MA) was the urinary biomarker with the greatest average level (127 ± 285 μg g−1 crt). Similar spatial trends can be observed between atmospheric benzene concentration and internal biomarker levels. The mean values of the LCR for male and female residents were 2.15 × 10−5 and 2.05 × 10−5, respectively. The results of the risk assessment indicated that special attention was required for the non-occupational residents around the area.
Growing up with a Chronically Ill Family Member—The Impact on and Support Needs of Young Adult Carers: A Scoping Review
Authors: van der Werf Hinke M. , Luttik Marie Louise A. , de Boer Alice , Roodbol Petrie F. , Paans Wolter
Published: January 2022

This scoping review provides an overview of the impact of growing up with a chronically ill family member on young adults (18–25 years old), and their specific needs. Young adults represent an important life stage involving a transition to adulthood, during which individuals’ family situations can affect their future. We searched relevant studies following the guideline of Arskey and O’Mailley’s methodological framework and the PRISMA statement guidelines for scoping reviews in PubMed, PsychInfo and reference lists to identify articles for inclusion. Studies from 2005 to 2020 were included in this review. Of the 12 studies, six qualitative studies, five quantitative studies and one mixed method study were included. Eight studies discussed the impact, including consequences at a physical and mental level, at their personal development and future perspectives, but also positive effects, such as being capable of organizing their lives. Four studies discussed the needs of young adult carers, including emotional needs, support needs with regard to stimulating autonomy (arising from internal conflicts) and developing their own identity, and the concerned attitude of involved professionals. An unambiguous definition of the target group and further well-designed research are needed to improve clarity about the role of support, so that future professionals can adequately address the needs and wishes of young adults who grow up with an ill family member.
Preparation and Characterization of a Novel Amidoxime-Modified Polyacrylonitrile/Fly Ash Composite Adsorbent and Its Application to Metal Wastewater Treatment
Authors: Sun Yan , Song Xiaojun , Ma Jing , Yu Haochen , Liu Gangjun , Chen Fu
Published: January 2022

The polyacrylonitrile/fly ash composite was synthesized through solution polymerization and was modified with NH2OH·HCl. The amidoxime-modified polyacrylonitrile/fly ash composite demonstrated excellent adsorption capacity for Zn2+ in an aqueous medium. Fourier transform-Infrared spectroscopy, thermogravimetric analysis, nitrogen adsorption, X-ray diffraction, and scanning electron microscopy were used to characterize the prepared materials. The results showed that the resulting amidoxime-modified polyacrylonitrile/fly ash composite was able to effectively remove Zn2+ at pH 4–6. Adsorption of Zn2+ was hindered by the coexisting cations. The adsorption kinetics of Zn2+ by Zn2+ followed the pseudo-second order kinetic model. The adsorption process also satisfactorily fit the Langmuir model, and the adsorption process was mainly single layer. The Gibbs free energy ΔG0, ΔH0, and ΔS0 were negative, indicating the adsorption was a spontaneous, exothermic, and high degree of order in solution system.
More than Mental Illness: Experiences of Associating with Stigma of Mental Illness for Chinese College Students
Authors: Yu Miao , Cheng Shengli , Fung Kenneth Po-Lun , Wong Josephine Pui-Hing , Jia Cunxian
Published: January 2022

From existing empirical research, we identified that Chinese college students commonly experience stigma surrounding mental illness and found some factors that support them in resisting the stigma and achieving psychological health. However, less research provides qualitative data involving individual experiences and insights on mental illness within this group of college students. This study, based on Linking Hearts (an internationally cooperative research-sharing project between China and Canada), was conducted in Shandong, Jinan, and aims to promote the mental health of college students by empowering interdisciplinary professionals and students. Through the research project, this study analyzed the materials from 24 focus groups, explored the understanding of mental illness and prevalence of mental illness stigma in Chinese colleges at the present time, administered a background questionnaire, and provided statistical support for some revealed themes. The final themes are as follows: mental illness is stereotyped as “severe, pathetic, and complicated”; the misconception of “visiting a psychological counselor is scary”; from public stigma to self-stigma; barriers deterring students from seeking help or accessing services; two sides of the same coin: peer support versus peer pressure.
Food Purchase Behavior during The First Wave of COVID-19: The Case of Hungary
Authors: Madarász Tamás , Kontor Enikő , Antal Emese , Kasza Gyula , Szakos Dávid , Szakály Zoltán
Published: January 2022

Coronavirus disease (SARSCoV-2) appeared in 2019 was confirmed as pandemic by the WHO on 11 March 2020. Stay-at-home order had an impact on consumers’ food purchase habits, as people around the world were able to leave their homes solely in extremely severe or urgent cases. In our research, we delve into the impact of COVID-19 pandemic on consumers’ food purchase habits. The research involved 3000 consumers during the first wave of coronavirus. The sample represents the Hungarian population by gender and age. To achieve the research goals, we applied multivariate statistical tools. The findings suggest that the pandemic could not change consumer attitude significantly, but the order of factors influencing purchases changed. Consumer motivation factors were organized into four well-distinguished factors: Healthy, domestic, and environmentally friendly choice; Usual taste and quality; Reasonable price; Shelf life. Due to the lack of outstanding data during segmentation, we developed four segments by hierarchical cluster analysis: Health- and environment-conscious women; Price sensitive young people; Taste-oriented men; Quality-oriented intellectuals. The results confirm that food manufacturers and traders need to be prepared for further restrictions in the future.
Contribution of Leisure Context, Motivation and Experience to the Frequency of Participation in Structured Leisure Activities among Adolescents
Authors: Belošević Matea , Ferić Martina
Published: January 2022

Leisure time is considered an important context for adolescent development. The purpose of this article is to investigate what contributes to the frequency of adolescents’ participation in structured leisure activities (SLA). Participants were aged 14–21 years (M = 18.87, SD = 1.23) and 44.8% of participants were female. Hierarchical regression analyses were conducted. Results indicate that boys and adolescents who perceive the context of participation in SLA as safe, are externally or intrinsically motivated, and perceive that participation has contributed to their identity development and experiences of initiative, as well as their experiences of stress, are more likely to participate frequently in SLA. On the other hand, these findings indicate that girls and adolescents who are unmotivated to participate in SLA and who experience negative peer influences while participating in SLA are less likely to frequently participate in SLA. It can be concluded that it is important to think much more broadly than just the setting of the activities themselves when promoting young people’s participation in SLA. Some of the features of SLA that promote positive youth development are presented in this paper.
Elite Triathlete Profiles in Draft-Legal Triathlons as a Basis for Talent Identification
Authors: Cuba-Dorado Alba , Álvarez-Yates Tania , García-García Oscar
Published: January 2022

Draft-legal triathlons are the main short-distance races worldwide and are those on which talent-identification programs are usually focused. Performance in these races depends on multiple factors; however, many investigations do not focus on elite triathletes. Therefore, the aim of this narrative review was to carry out a systematic literature search to define the elite female and male triathlete profiles and their competition demands in draft-legal triathlons. This will allow us to summarize the main determinant factors of high-level triathletes as a basis for talent detection. A comprehensive review of Web of Science and Scopus was performed using the search strategy: Triathl* and (performance or competition or profile) and (elite or professional or “high performance” or “high level” or talent). A total of 1325 research documents were obtained, and after screening following the criteria, only 83 articles were selected. After data synthesis, elite triathlete aspects such as age, physiological, anthropometric, and psychosocial profile or competition demands were studied in the scientific literature. Thus, it is essential that when implementing talent identification programs, these factors must be considered. However, constant updating is needed due the continuous regulatory changes and the need of triathletes to adapt to these new competition demands.
Development and Evaluation of Cultural Competence Course on Undergraduate Nursing Students in Vietnam
Authors: Ho Trang-Thi-Thuy , Oh Jina
Published: January 2022

Cultural competence is a crucial requirement of nursing to promote caring for patients with diverse backgrounds. The purpose of this study was to develop a cultural competence course and to evaluate the effects of the course on undergraduate nursing students in Vietnam. A concurrent triangulation mixed-methods study was adopted using quantitative and qualitative data sources. Sixty-six nursing students were recruited for the following groups: cultural competence course with field experience (n = 22), stand-alone cultural competence course (n = 22), and a control group (n = 22). The findings indicated that significant group by time interactions in total cultural competence score (F = 66.73, p < 0.001) were found. Participants’ perceptions reflected on three categories: (a) journey to cultural competence, (b) satisfaction of cultural competence course, and (c) suggestions for improvements. No statistically significant differences between the two experimental groups were revealed, but “obtaining cultural experiences” and “expanding understanding of cultural competence through field experience” were immersed from participants having field experience. It is vital to expand cultural competency education into nursing curricula to enhance nursing students’ perspective of culturally competent care.
Textural and Thermal Properties of the Novel Fucoidan/Nano-Oxides Hybrid Materials with Cosmetic, Pharmaceutical and Environmental Potential
Authors: Matusiak Jakub , Maciołek Urszula , Kosińska-Pezda Małgorzata , Sternik Dariusz , Orzeł Jolanta , Grządka Elżbieta
Published: January 2022

The main purpose of the research was to obtain and study hybrid materials based on three different nano-oxides commonly used in the cosmetic and pharmaceutical industries: Al2O3, TiO2, and ZnO, with the natural bioactive polysaccharide fucoidan. Since the mentioned oxides are largely utilized by industry, there is no doubt that the presented studies are important from an environmental point of view. On the basis of the textural studies (dynamic light scattering DLS, low temperature nitrogen adsorption, X-ray diffraction analysis XRD, scanning electron microscopy SEM) it was proved that the properties of the hybrid materials differ from the pure components of the system. Moreover, the advanced thermal analysis (TG-DTG-DSC) combined with the evolved gas analysis using Fourier transformed infrared spectroscopy (FTIR) and mass spectrometry were applied to describe the thermal decomposition of fucoidan, oxides and hybrid materials. It was found that the interactions between the polymer and the oxides results in the formation of the hybrid materials due to the functionalization of the nanoparticles surface, and that their thermal stability increased when compared to the pure substrates. Such findings definitely fill the literature void regarding the fucoidan based hybrid materials and help the industrial formulators in the preparation of new products.
Erinacine A Prevents Lipopolysaccharide-Mediated Glial Cell Activation to Protect Dopaminergic Neurons against Inflammatory Factor-Induced Cell Death In Vitro and In Vivo
Authors: Lee Shou-Lun , Hsu Jing-Ya , Chen Ting-Chun , Huang Chun-Chih , Wu Tzong-Yuan , Chin Ting-Yu
Published: January 2022

Hericium erinaceus (HE) is a common edible mushroom consumed in several Asian countries and considered to be a medicinal mushroom with neuroprotective effects. Erinacine A (EA) is a bioactive compound in Hericium erinaceus mycelium (HEM) that has been shown to have a neuroprotective effect against neurodegenerative diseases, e.g., Parkinson’s disease (PD). Although the etiology of PD is still unclear, neuroinflammation may play an important role in causing dopaminergic neuron loss, which is a pathological hallmark of PD. However, glial cell activation has a close relationship with neuroinflammation. Thus, this study aimed to investigate the anti-neuroinflammatory and neuroprotective effects of EA on lipopolysaccharide (LPS)-induced glial cell activation and neural damage in vitro and in vivo. For the in vitro experiments, glial cells, BV-2 microglial cells and CTX TNA2 astrocytes were pretreated with EA and then stimulated with LPS and/or IFN-γ. The expression of proinflammatory factors in the cells and culture medium was analyzed. In addition, differentiated neuro-2a (N2a) cells were pretreated with EA or HEM and then stimulated with LPS-treated BV-2 conditioned medium (CM). The cell viability and the amount of tyrosine hydroxylase (TH) and mitogen-activated protein kinases (MAPKs) were analyzed. In vivo, rats were given EA or HEM by oral gavage prior to injection of LPS into the substantia nigra (SN). Motor coordination of the rats and the expression of proinflammatory mediators in the midbrain were analyzed. EA pretreatment prevented LPS-induced iNOS expression and NO production in BV-2 cells and TNF-α expression in CTX TNA2 cells. In addition, both EA and HEM pretreatment significantly increased cell viability and TH expression and suppressed the phosphorylation of JNK and NF- κB in differentiated N2a cells treated with CM. In vivo, both EA and HEM significantly improved motor dysfunction in the rotarod test and the amphetamine-induced rotation test and reduced the expression of TNF-α, IL-1β and iNOS in the midbrain of rats intranigrally injected with LPS. The results demonstrate that EA ameliorates LPS-induced neuroinflammation and has neuroprotective properties.
Control of Expression of Key Cell Cycle Enzymes Drives Cell Line-Specific Functions of CDK7 in Human PDAC Cells
Authors: Kolloch Lina , Kreinest Teresa , Meisterernst Michael , Oeckinghaus Andrea
Published: January 2022

Inhibition of the dual function cell cycle and transcription kinase CDK7 is known to affect the viability of cancer cells, but the mechanisms underlying cell line-specific growth control remain poorly understood. Here, we employed a previously developed, highly specific small molecule inhibitor that non-covalently blocks ATP binding to CDK7 (LDC4297) to study the mechanisms underlying cell line-specific growth using a panel of genetically heterogeneous human pancreatic tumor lines as model system. Although LDC4297 diminished both transcription rates and CDK T-loop phosphorylation in a comparable manner, some PDAC lines displayed significantly higher sensitivity than others. We focused our analyses on two well-responsive lines (Mia-Paca2 and Panc89) that, however, showed significant differences in their viability upon extended exposure to limiting LDC4297 concentrations. Biochemical and RNAseq analysis revealed striking differences in gene expression and cell cycle control. Especially the downregulation of a group of cell cycle control genes, among them CDK1/2 and CDC25A/C, correlated well to the observed viability differences in Panc89 versus Mia-Paca2 cells. A parallel downregulation of regulatory pathways supported the hypothesis of a feedforward programmatic effect of CDK7 inhibitors, eventually causing hypersensitivity of PDAC lines.
The Emerging Role of Non-Coding RNAs in the Regulation of Virus Replication and Resultant Cellular Pathologies
Authors: Ghafouri-Fard Soudeh , Hussen Bashdar Mahmud , Jamal Hazha Hadayat , Taheri Mohammad , Sharifi Guive
Published: January 2022

Non-coding RNAs, particularly lncRNAs and miRNAs, have recently been shown to regulate different steps in viral infections and induction of immune responses against viruses. Expressions of several host and viral lncRNAs have been found to be altered during viral infection. These lncRNAs can exert antiviral function via inhibition of viral infection or stimulation of antiviral immune response. Some other lncRNAs can promote viral replication or suppress antiviral responses. The current review summarizes the interaction between ncRNAs and herpes simplex virus, cytomegalovirus, and Epstein–Barr infections. The data presented in this review helps identify viral-related regulators and proposes novel strategies for the prevention and treatment of viral infection.
A New RING Finger Protein, PLANT ARCHITECTURE and GRAIN NUMBER 1, Affects Plant Architecture and Grain Yield in Rice
Authors: Yan Peiwen , Zhu Yu , Wang Ying , Ma Fuying , Lan Dengyong , Niu Fuan , Dong Shiqing , Zhang Xinwei , Hu Jian , Liu Siwen , Guo Tao , Xin Xiaoyun , Zhang Shiyong , Yang Jinshui , Cao Liming , Luo Xiaojin
Published: January 2022

Developing methods for increasing the biomass and improving the plant architecture is important for crop improvement. We herein describe a gene belonging to the RING_Ubox (RING (Really Interesting New Gene) finger domain and U-box domain) superfamily, PLANT ARCHITECTURE and GRAIN NUMBER 1 (PAGN1), which regulates the number of grains per panicle, the plant height, and the number of tillers. We used the CRISPR/Cas9 system to introduce loss-of-function mutations to OsPAGN1. Compared with the control plants, the resulting pagn1 mutant plants had a higher grain yield because of increases in the plant height and in the number of tillers and grains per panicle. Thus, OsPAGN1 may be useful for the genetic improvement of plant architecture and yield. An examination of evolutionary relationships revealed that OsPAGN1 is highly conserved in rice. We demonstrated that OsPAGN1 can interact directly with OsCNR10 (CELL NUMBER REGULATOR10), which negatively regulates the number of rice grains per panicle. A transcriptome analysis indicated that silencing OsPAGN1 affects the levels of active cytokinins in rice. Therefore, our findings have clarified the OsPAGN1 functions related to rice growth and grain development.
Immunologic Effects of Stereotactic Body Radiotherapy in Dogs with Spontaneous Tumors and the Impact of Intratumoral OX40/TLR Agonist Immunotherapy
Authors: Boss Mary-Keara , Watts Remy , Harrison Lauren G. , Hopkins Sophie , Chow Lyndah , Trageser Erin , Easton Carina , LaRue Susan M. , Regan Daniel , Dewhirst Mark W. , Dow Steven
Published: January 2022

Stereotactic body radiotherapy (SBRT) is known to induce important immunologic changes within the tumor microenvironment (TME). However, little is known regarding the early immune responses within the TME in the first few weeks following SBRT. Therefore, we used the canine spontaneous tumor model to investigate TME responses to SBRT, and how local injection of immune modulatory antibodies to OX40 and TLR 3/9 agonists might modify those responses. Pet dogs with spontaneous cancers (melanoma, carcinoma, sarcoma, n = 6 per group) were randomized to treatment with either SBRT or SBRT combined with local immunotherapy. Serial tumor biopsies and serum samples were analyzed for immunologic responses. SBRT alone resulted at two weeks after treatment in increased tumor densities of CD3+ T cells, FoxP3+ Tregs, and CD204+ macrophages, and increased expression of genes associated with immunosuppression. The addition of OX40/TLR3/9 immunotherapy to SBRT resulted in local depletion of Tregs and tumor macrophages and reduced Treg-associated gene expression (FoxP3), suppressed macrophage-associated gene expression (IL-8), and suppressed exhausted T cell-associated gene expression (CTLA4). Increased concentrations of IL-7, IL-15, and IL-18 were observed in serum of animals treated with SBRT and immunotherapy, compared to animals treated with SBRT. A paradoxical decrease in the density of effector CD3+ T cells was observed in tumor tissues that received combined SBRT and immunotherapy as compared to animals treated with SBRT only. In summary, these results obtained in a spontaneous large animal cancer model indicate that addition of OX40/TLR immunotherapy to SBRT modifies important immunological effects both locally and systemically.
Pharmacological Dissection of the Crosstalk between NaV and CaV Channels in GH3b6 Cells
Authors: Réthoré Léa , Park Joohee , Montnach Jérôme , Nicolas Sébastien , Khoury Joseph , Le Seac’h Elodie , Mabrouk Kamel , De Pomyers Harold , Tricoire-Leignel Hélène , Mattei César , Henrion Daniel , Fajloun Ziad , De Waard Michel , Legendre Claire , Legros Christian
Published: January 2022

Thanks to the crosstalk between Na+ and Ca2+ channels, Na+ and Ca2+ homeostasis interplay in so-called excitable cells enables the generation of action potential in response to electrical stimulation. Here, we investigated the impact of persistent activation of voltage-gated Na+ (NaV) channels by neurotoxins, such as veratridine (VTD), on intracellular Ca2+ concentration ([Ca2+]i) in a model of excitable cells, the rat pituitary GH3b6 cells, in order to identify the molecular actors involved in Na+-Ca2+ homeostasis crosstalk. By combining RT-qPCR, immunoblotting, immunocytochemistry, and patch-clamp techniques, we showed that GH3b6 cells predominantly express the NaV1.3 channel subtype, which likely endorses their voltage-activated Na+ currents. Notably, these Na+ currents were blocked by ICA-121431 and activated by the β-scorpion toxin Tf2, two selective NaV1.3 channel ligands. Using Fura-2, we showed that VTD induced a [Ca2+]i increase. This effect was suppressed by the selective NaV channel blocker tetrodotoxin, as well by the selective L-type CaV channel (LTCC) blocker nifedipine. We also evidenced that crobenetine, a NaV channel blocker, abolished VTD-induced [Ca2+]i elevation, while it had no effects on LTCC. Altogether, our findings highlight a crosstalk between NaV and LTCC in GH3b6 cells, providing a new insight into the mode of action of neurotoxins.
Ovarian Cancer Cells in Ascites Form Aggregates That Display a Hybrid Epithelial-Mesenchymal Phenotype and Allows Survival and Proliferation of Metastasizing Cells
Authors: Capellero Sonia , Erriquez Jessica , Battistini Chiara , Porporato Roberta , Scotto Giulia , Borella Fulvio , Di Renzo Maria F. , Valabrega Giorgio , Olivero Martina
Published: January 2022

Peritoneal metastases are the leading cause of morbidity and mortality in ovarian cancer. Cancer cells float in peritoneal fluid, named ascites, together with a definitely higher number of non neo-neoplastic cells, as single cells or multicellular aggregates. The aim of this work is to uncover the features that make these aggregates the metastasizing units. Immunofluorescence revealed that aggregates are made almost exclusively of ovarian cancer cells expressing the specific nuclear PAX8 protein. The same cells expressed epithelial and mesenchymal markers, such as EPCAM and αSMA, respectively. Expression of fibronectin further supported a hybrid epithelia-mesenchymal phenotype, that is maintained when aggregates are cultivated and proliferate. Hematopoietic cells as well as macrophages are negligible in the aggregates, while abundant in the ascitic fluid confirming their prominent role in establishing an eco-system necessary for the survival of ovarian cancer cells. Using ovarian cancer cell lines, we show that cells forming 3D structures neo-expressed thoroughly fibronectin and αSMA. Functional assays showed that αSMA and fibronectin are necessary for the compaction and survival of 3D structures. Altogether these data show that metastasizing units display a hybrid phenotype that allows maintenance of the 3D structures and the plasticity necessary for implant and seeding into peritoneal lining.
Developmental Formation of the GABAergic and Glycinergic Networks in the Mouse Spinal Cord
Authors: Shimizu-Okabe Chigusa , Kobayashi Shiori , Kim Jeongtae , Kosaka Yoshinori , Sunagawa Masanobu , Okabe Akihito , Takayama Chitoshi
Published: January 2022

Gamma-aminobutyric acid (GABA) and glycine act as inhibitory neurotransmitters. Three types of inhibitory neurons and terminals, GABAergic, GABA/glycine coreleasing, and glycinergic, are orchestrated in the spinal cord neural circuits and play critical roles in regulating pain, locomotive movement, and respiratory rhythms. In this study, we first describe GABAergic and glycinergic transmission and inhibitory networks, consisting of three types of terminals in the mature mouse spinal cord. Second, we describe the developmental formation of GABAergic and glycinergic networks, with a specific focus on the differentiation of neurons, formation of synapses, maturation of removal systems, and changes in their action. GABAergic and glycinergic neurons are derived from the same domains of the ventricular zone. Initially, GABAergic neurons are differentiated, and their axons form synapses. Some of these neurons remain GABAergic in lamina I and II. Many GABAergic neurons convert to a coreleasing state. The coreleasing neurons and terminals remain in the dorsal horn, whereas many ultimately become glycinergic in the ventral horn. During the development of terminals and the transformation from radial glia to astrocytes, GABA and glycine receptor subunit compositions markedly change, removal systems mature, and GABAergic and glycinergic action shifts from excitatory to inhibitory.
Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2
Authors: Biswas Sudip , Wahl Nancy J. , Thomson Michael J. , Cason John M. , McCutchen Bill F. , Septiningsih Endang M.
Published: January 2022

The cultivated peanut (Arachis hypogaea L.) is a legume consumed worldwide in the form of oil, nuts, peanut butter, and candy. Improving peanut production and nutrition will require new technologies to enable novel trait development. Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR–Cas9) is a powerful and versatile genome-editing tool for introducing genetic changes for studying gene expression and improving crops, including peanuts. An efficient in vivo transient CRISPR–Cas9- editing system using protoplasts as a testbed could be a versatile platform to optimize this technology. In this study, multiplex CRISPR–Cas9 genome editing was performed in peanut protoplasts to disrupt a major allergen gene with the help of an endogenous tRNA-processing system. In this process, we successfully optimized protoplast isolation and transformation with green fluorescent protein (GFP) plasmid, designed two sgRNAs for an allergen gene, Ara h 2, and tested their efficiency by in vitro digestion with Cas9. Finally, through deep-sequencing analysis, several edits were identified in our target gene after PEG-mediated transformation in protoplasts with a Cas9 and sgRNA-containing vector. These findings demonstrated that a polyethylene glycol (PEG)-mediated protoplast transformation system can serve as a rapid and effective tool for transient expression assays and sgRNA validation in peanut.
Computational Design of Miniproteins as SARS-CoV-2 Therapeutic Inhibitors
Authors: Jawad Bahaa , Adhikari Puja , Cheng Kun , Podgornik Rudolf , Ching Wai-Yim
Published: January 2022

A rational therapeutic strategy is urgently needed for combating SARS-CoV-2 infection. Viral infection initiates when the SARS-CoV-2 receptor-binding domain (RBD) binds to the ACE2 receptor, and thus, inhibiting RBD is a promising therapeutic for blocking viral entry. In this study, the structure of lead antiviral candidate binder (LCB1), which has three alpha-helices (H1, H2, and H3), is used as a template to design and simulate several miniprotein RBD inhibitors. LCB1 undergoes two modifications: structural modification by truncation of the H3 to reduce its size, followed by single and double amino acid substitutions to enhance its binding with RBD. We use molecular dynamics (MD) simulations supported by ab initio density functional theory (DFT) calculations. Complete binding profiles of all miniproteins with RBD have been determined. The MD investigations reveal that the H3 truncation results in a small inhibitor with a −1.5 kcal/mol tighter binding to RBD than original LCB1, while the best miniprotein with higher binding affinity involves D17R or E11V + D17R mutation. DFT calculations provide atomic-scale details on the role of hydrogen bonding and partial charge distribution in stabilizing the minibinder:RBD complex. This study provides insights into general principles for designing potential therapeutics for SARS-CoV-2.
Cancer Therapy by Silver Nanoparticles: Fiction or Reality?
Authors: Kovács Dávid , Igaz Nóra , Gopisetty Mohana K. , Kiricsi Mónika
Published: January 2022

As an emerging new class, metal nanoparticles and especially silver nanoparticles hold great potential in the field of cancer biology. Due to cancer-specific targeting, the consequently attenuated side-effects and the massive anti-cancer features render nanoparticle therapeutics desirable platforms for clinically relevant drug development. In this review, we highlight those characteristics of silver nanoparticle-based therapeutic concepts that are unique, exploitable, and achievable, as well as those that represent the critical hurdle in their advancement to clinical utilization. The collection of findings presented here will describe the features that distinguish silver nanoparticles from other anti-cancer agents and display the realistic opportunities and implications in oncotherapeutic innovations to find out whether cancer therapy by silver nanoparticles is fiction or reality.
Irreversible and Self-Healing Electrically Conductive Hydrogels Made of Bio-Based Polymers
Authors: Nada Ahmed Ali , Eckstein Andicsová Anita , Mosnáček Jaroslav
Published: January 2022

Electrically conductive materials that are fabricated based on natural polymers have seen significant interest in numerous applications, especially when advanced properties such as self-healing are introduced. In this article review, the hydrogels that are based on natural polymers containing electrically conductive medium were covered, while both irreversible and reversible cross-links are presented. Among the conductive media, a special focus was put on conductive polymers, such as polyaniline, polypyrrole, polyacetylene, and polythiophenes, which can be potentially synthesized from renewable resources. Preparation methods of the conductive irreversible hydrogels that are based on these conductive polymers were reported observing their electrical conductivity values by Siemens per centimeter (S/cm). Additionally, the self-healing systems that were already applied or applicable in electrically conductive hydrogels that are based on natural polymers were presented and classified based on non-covalent or covalent cross-links. The real-time healing, mechanical stability, and electrically conductive values were highlighted.
Is There a Link between Oropharyngeal Microbiome and Schizophrenia? A Narrative Review
Authors: Martin Stanislas , Foulon Audrey , El Hage Wissam , Dufour-Rainfray Diane , Denis Frédéric
Published: January 2022

The study aimed to examine the impact of the oropharyngeal microbiome in the pathophysiology of schizophrenia and to clarify whether there might be a bidirectional link between the oral microbiota and the brain in a context of dysbiosis-related neuroinflammation. We selected nine articles including three systemic reviews with several articles from the same research team. Different themes emerged, which we grouped into 5 distinct parts concerning the oropharyngeal phageome, the oropharyngeal microbiome, the salivary microbiome and periodontal disease potentially associated with schizophrenia, and the impact of drugs on the microbiome and schizophrenia. We pointed out the presence of phageoma in patients suffering from schizophrenia and that periodontal disease reinforces the role of inflammation in the pathophysiology of schizophrenia. Moreover, saliva could be an interesting substrate to characterize the different stages of schizophrenia. However, the few studies we have on the subject are limited in scope, and some of them are the work of a single team. At this stage of knowledge, it is difficult to conclude on the existence of a bidirectional link between the brain and the oral microbiome. Future studies on the subject will clarify these questions that for the moment remain unresolved.
Two-Dimensional Interfacial Exchange Diffusion Has the Potential to Augment Spatiotemporal Precision of Ca2+ Signaling
Authors: van Breemen Cornelis , Fameli Nicola , Groschner Klaus
Published: January 2022

Nano-junctions between the endoplasmic reticulum and cytoplasmic surfaces of the plasma membrane and other organelles shape the spatiotemporal features of biological Ca2+ signals. Herein, we propose that 2D Ca2+ exchange diffusion on the negatively charged phospholipid surface lining nano-junctions participates in guiding Ca2+ from its source (channel or carrier) to its target (transport protein or enzyme). Evidence provided by in vitro Ca2+ flux experiments using an artificial phospholipid membrane is presented in support of the above proposed concept, and results from stochastic simulations of Ca2+ trajectories within nano-junctions are discussed in order to substantiate its possible requirements. Finally, we analyze recent literature on Ca2+ lipid interactions, which suggests that 2D interfacial Ca2+ diffusion may represent an important mechanism of signal transduction in biological systems characterized by high phospholipid surface to aqueous volume ratios.
Novel Diagnostic Biomarkers in Colorectal Cancer
Authors: Zygulska Aneta L. , Pierzchalski Piotr
Published: January 2022

Colorectal cancer (CRC) is still a leading cause of cancer death worldwide. Less than half of cases are diagnosed when the cancer is locally advanced. CRC is a heterogenous disease associated with a number of genetic or somatic mutations. Diagnostic markers are used for risk stratification and early detection, which might prolong overall survival. Nowadays, the widespread use of semi-invasive endoscopic methods and feacal blood tests characterised by suboptimal accuracy of diagnostic results has led to the detection of cases at later stages. New molecular noninvasive tests based on the detection of CRC alterations seem to be more sensitive and specific then the current methods. Therefore, research aiming at identifying molecular markers, such as DNA, RNA and proteins, would improve survival rates and contribute to the development of personalized medicine. The identification of “ideal” diagnostic biomarkers, having high sensitivity and specificity, being safe, cheap and easy to measure, remains a challenge. The purpose of this review is to discuss recent advances in novel diagnostic biomarkers for tumor tissue, blood and stool samples in CRC patients.