bluuebunny/crossref_metadata_2025 huggingface dataset:
ISOLATION AND IDENTIFICATION OF A NEW ANTIPROLIFERATIVE INDOLOCARBAZOLE ALKALOID DERIVATIVE EXTRACTED FROM FARMED SHRIMP (Litopenaeus vannamei) MUSCLE
Authors: García-Romo Joel-Said , Hernández-Zazueta Martín-Samuel , Gálvez-Iriqui Alma-Carolina , Plascencia-Jatomea Maribel , Burboa-Zazueta María-Guadalupe , Sandoval-Petris Edgar , Robles-Sánchez Rosario-Maribel , Juárez-Onofre Josué-Elías , Hernández-Martínez Javier , Santacruz-Ortega Hisila del Carmen , López-Saiz Carmen-María , Burgos-Hernández Armando
Published: February 2022

Farmed shrimp Litopenaeus vannamei, one of the most consumed seafood in the planet, is a source of antiproliferative extracts still to be fully characterized. This study the isolation and identification of these antiproliferative compounds. From a chloroform-soluble extract from shrimp muscle, hexane- and methanol-soluble fractions were obtained and tested for antiproliferative activity (MTT), a bioassay that guided the fractionation and isolation of bioactive fractions using open column chromatography. MeOH-soluble fraction resulted bioactive and was subjected to further fractionation from which one subfraction outstand for being highly active against prostate cancer cell line. Antiproliferative effects were evaluated using colorimetric assays and cell morphology observations. Further chromatographic procedures resulted in sub-fractions from which one was effective in causing DNA damage and F-actin polymerization, which suggests cellular collapse and apoptosis. According to the structural chemical characterization carried out, dioctyl phthalate, eicosapentaenoic acid, and an indolocarbazole alkaloid type of compound were identified. This last compound, which resulted majorly responsible for the bioactivity, was not found reported in the available databases. Pure EPA control was used to compare it with the subfraction, observing greater activity in the subfraction than when EPA was used, suggesting that another compound different from EPA is providing the highest activity; but, more investigation is needed for a full chemical and structural characterization.
MICROPROPAGATION AND ORGANOGENESIS OF ASTROPHYTUM ASTERIAS (ZUCC.) LEM. (CACTACEAE JUSS.), BLOSSFELDIA LILIPUTIANA WERDERM. AND STROMBOCACTUS DISCIFORMIS (DC.) BRITTON & ROSE
Authors: Ivannikov Roman , Lobova Oksana , Ivannikova Natalia , Krasnienkova Iryna
Published: February 2022

The research reveals results of the creation of sterile cultures and micropropagation of the species of three unique genera of Cactaceae: Astrophytum asterias (Zucc.) Lem. (І CITES); Blossfeldia liliputiana Werderm. (ІІ CITES); Strombocactus disciformis (DC.) Britton & Rose. (І CITES). The solutions for sterilization were selected, the time of sterilization, and the sterilization procedure of seeds was approbated. The expedience of usage of stems of juvenile plants grown in vitro for propagation of these species of cacti was proved. The possibility of an obtaining of the necessary quantity of plant material by the way of indirect organogenesis is shown. The proper content of nutrient media and favourable conditions of the cultivation for all stages of the micropropagation were approved.
PROBIOTIC AND NUTRITIONAL CONTENT OF SOURO, AN IRANIAN TRADITIONAL FOOD
Authors: Mohkam Milad , Ghasemi Firoozabadi Shiva , Ghasemi Younes , Gholami Ahmad
Published: February 2022

Probiotic foods are products containing adequate amounts of live and specific microorganisms that confer benefit to human health. This research was performed to spotlight on the nutritional value evaluation as well as isolation and probiotic characterization of lactic acid bacteria (LAB) isolated from native food "Souro" belonging to Iran. The isolated LABs were investigated in vitro for potential probiotic features based on their acidic pH tolerance, bile-salt resistance, hydrophobicity, autoaggragation, production of amylase and protease as well as antibiotic susceptibility test. Results showed that Souro had high content of protein (51 ± 0.16%,) and total lipid was 11.18 ± 0.38%. Most of isolates were able to produce protease and amylase. From the best potential probiotic isolates, 16S rRNA gene sequencing disclosed Lactobacillus plantarum, L. hilgardii, L. casei, L. fermentum and Weissella paramesenteroides. Thus, good physiological traits and safety evaluation of the isolated LAB emphasized the benefits of Souro for further use in the human diet as well as the preparation of diverse probiotic food formulations.
SUBTILISIN AN EMINENT MICROBIAL PRODUCT AS A POTENTIAL SKIN CARE REGULATOR OF MELANOGENESIS –A PARADIGM SYNCHRONIZED WITH IN VITRO / IN SILICO APPROACH
Authors: Margret Arockiya Anita , Nivetha Irulandi , Jerley Arockiya Avila , Aishwarya S
Published: February 2022

Colour is considered as a foremost parameter for physical appearance and personal identification. The distinct coloration of skin, hair and eyes of humas are categorized based on the natural pigment melanin which is the final product of melanogenesis. Melanin establishes a primary protection to the skin by inducing photo protection. But the rapid mechanism of absorbing free radicals from the cytoplasm is defensive against UV light, thereby its enormous production   and accretion leads to perturbing skin ailments. During the process of melanogenesis the core enzyme tyrosinase is employed and moderated by a main transcription factor called microphthalmia associated transcription factor (MITF), which is enhanced by both tyrosinase-related 1 and 2 proteins (TRP-1 /TRP-2). Amending this physiological process is considered to be the foremost mechanism in improving skin fairness. Conversely, there are numerous cosmetics commercially available to depigment skin colour, the alarm of adverse effects and reoccurrence of hyperpigmentation prevails as a hitch. Synthetic skin products cause remedy with adverse effects and hence there is a high demand for novel skin colouring agent. This work lays a pedal stone in promoting the appliance of a naturally derived protein subtilisin secluded from soil isolates of Bacillus sp. in cosmetic industry as skin whitening aspect. The extraction of subtilisin was detected by biochemical and HPLC assays coordinated with anti-melanogenesis activity by in vitro studies. Further, synchronization of   molecular docking studies hoisted the protein as an effective ligand targeting TRP1 and TRP2 with energy minimization of -675569 KJ/Mol and -36957KJ/Mol.
PECULIARITIES OF FORMATION AND FUNCTIONING OF THE SOYBEAN–BRADYRHIZOBIUM JAPONICUM SYMBIOTIC APPARATUS IN RELATION TO PHOTOSYNTHETIC ACTIVITY UNDER THE INFLUENCE OF SEED PROTECTANTS
Authors: Kots Serhii , Kiriziy Dmytro , Pavlyshche Anastasiia , Rybachenko Liliia
Published: February 2022

The aim of work was to investigate the influence of seed treatment with two preparations of fungicidal action, differing in the active substances composition, on the formation and functioning of legume-rhizobial symbiosis in interaction with the photosynthetic apparatus of soybean plants. It was established that in plants treated with preparation Fever, the nitrogen-fixing activity (NFA) difference with the control was insignificant, but under the impact of the preparation Standak Top NFA was by 63% less at the stage of three true leaves, by 48% – at the stage of budding, and by 34% – at the stage of budding–flowering. The net photosynthetic rate, calculated per leaf area unit, was lower than control at all investigated stages of soybean plants development in the variants with the seed protectants applying. A fairly close positive correlations (R2 = 0.79) were observed between the average weight of one nodule and its NFA, and between the last and total NFA of the whole plant (R2 = 0.78). Close correlations between plant NFA, chlorophyll content in leaves, and net photosynthetic rate were revealed. It was concluded that seed protectants significantly influenced on nitrogen fixation and photosynthesis.
The strength of manifestation, and the sign of this influence in comparison with the relevant parameters of the control plants depended on the preparation, the stage of plant development, as well as on the index (nodules formation, nitrogen-fixing activity, photosynthetic, chlorophyll content, chlorophyll index, biological productivity of soybean plants) under consideration.
FOOD PATTERN LINK TO COVID19: THE ROLE OF DIET AND NUTRITIONAL SUPPLEMENT DURING A WORLD WIDE HEALTH CATASTROPHE
Authors: Baisya Sanchita , Saha Abinit , Paul Joydeep , Saha Rudra , DAS ARPITA
Published: February 2022

Nutritional diet and essential bioactive supplements can shape out to be a vital technique to attain healthy and well-balanced body functioning. Unhealthy dietary habits can result into various physical ailment and one becomes prone to various disease because of the same. COVID-19 infection depends on a two-phase immune response and the risk of infection or overcoming the infection is higher for stronger immune system. In this piece of writing we have correlated the properties of certain immunity strengthening food against the risk of various viral infections. We had also conducted a survey with people living all across India to study the change in food pattern and preferences during this pandemic lockdown situation and have presented them in graphical format. A healthy diet consisting of various components like minerals, zinc, iron, vitamin C, vitamin D, vitamin E has been seen to improve immune system and strengthens it.
A REVIEW OF CURRENT KNOWLEDGE AND GAPS ABOUT CAMPYLOBACTER METHODS: FROM CULTURE TO CHARACTERIZATION
Authors: El Baaboua Aicha , El maadoudi Mohamed , Bouyahya Abdelhakim , Kounnoun Ayoub , Bougtaib Hajar , Omar Belmehdi , Boujida Nadia , Abrini Jamal
Published: February 2022

Campylobacteriosis is a zoonotic disease caused by Campylobacter, mostly associated with consumption of contaminated foodstuffs and water. Campylobacter jejuni and Campylobacter coli recognized as the leader of foodborne diarrheal illness in humans. The frequency of these microorganisms in poultry is fairly high than Salmonella and more challenging to measure, which represent an expensive burden charge on public health due to their difficulties to master them, especially with the fast increase rates of multidrug-resistant of thermophilic Campylobacter strains. It is well recognized that Campylobacter spp. is a fastidious cell, difficult to isolate in laboratories owing to their requirements and sensibility. That’s why; these factors must be taken into consideration during recovery protocols. A variety of phenotyping tests have been reported and widely used for confirmation and identification of Campylobacter species. Nonetheless, Whole Genome Sequencing (WGS) and Culture-Independent Diagnostic Tests (CIDTs) are new eras of hopeful technologies, mainly involved in the detection and characterization of threaten public health pathogens. This review aimed to describe the culture methods, phenotypic and genotypic schemes used to isolate, identify, and characterize Campylobacter isolates, through discussing the current knowledge and gaps related to the application of these techniques over others performed for typing this microaerophilic genus.
TRIAZOLE RESISTANCE IN ASPERGILLUS FUMIGATUS-A COMPREHENSIVE REVIEW
Authors: Wadhwa Khushbu , Kaur Hardeep
Published: February 2022

Aspergillus fumigatus is a common ubiquitous fungal pathogen present in the environment including soil, decaying leaves, compost, grain crops and household dust. It can turn opportunistic in immunocompromised individuals causing allergic diseases, respiratory problems and invasive blood stream infections. Being saprophytic, it propagates by producing minute grey-green conidia that can become airborne and reach humans. In majority of cases, aspergillosis due to this organism can heal with treatment by antifungals. The azole compounds, one of the most important antifungal drugs, generally target the 14-α sterol demethylase enzyme, encoded by the cyp51A gene of the fungus, causing impaired membrane ergosterol synthesis. However in recent years the situation has turned grave due to development of azole resistant fungal species both in the environment as well as in the clinics. The main mechanism of resistance in A. fumigatus involves incorporation of tandem repeats in the promoter region of cyp51A, along with point mutations in the gene sequence. Resistant isolates tend to arise either due to excessive use of azole fungicides in the agricultural fields, from where they reach human through sporulation; or by prolonged treatment of patients with azole drugs. The early diagnosis of aspergillosis is very crucial for successful treatment of a patient. The article brings forth a comprehensive study of this organism, the mechanism of development and spread of azole resistant fungal isolates, genetic basis of development of azole resistance, detection of resistant strains and finally drugs used to combat the fungal infection.
THE EFFECTS OF ORIENTIN ON THP-1 CELLS INFECTED BY DENV2
Authors: Tune Bernadette Xin-Jie , Ling Anna PK , Voon Kenny Gah Leong , Wong Ying
Published: February 2022

Orientin, a flavonoid known for its significant antioxidative properties, has also shown to possess antiviral properties against some viruses. Nevertheless, up to now, there was no report on its ability against dengue virus. This study investigated the effects of orientin against DENV2 infecting human monocyte, THP-1 cells. THP-1 cells infected with DENV2 (3.16×102 TCID50/mL) were treated with a maximum non-toxic dose (MNTD) of orientin (0.053 ± 0.006 µM) for two days. Cytopathic effect (CPE) formation was observed after two days, followed by measurement of the percentage of cell viability using 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The treatment was compared to cells infected with DENV2 only, cells treated with orientin at MNTD only, and control cells. The MNTD of orientin on THP-1 cells were determined to be 0.053 ± 0.006 µM. Treatment of DENV2 infected THP-1 cells with the MNTD of orientin showed CPE formation similar to the THP-1 cells infected with DENV2 only. The MTT assay showed that orientin did not exhibit antiviral activity against DENV2 infecting THP-1 cells. Percentage of cell viability of the orientin treated cells and DENV2-infected cells were not significantly different. Orientin at MNTD does not exhibit antiviral effects on THP-1 cells infected with DENV2.
EFFECTS OF WHOLE FRUIT EXTRACT OF ELAEAGNUS ANGUSTIFOLIA L. ON GLIOBLASTOMA CELL LINES
Authors: Arab Samaneh , Bahraminasab Marjan , Yazdani Alireza , Abdolshahi Anna
Published: February 2022

Elaeagnus. angustifolia L. (EA) is a natural plant food with therapeutic and medicinal properties for treating various diseases such as rheumatoid arthritis and osteoporosis. Since herbal agents are origins of bioactive compounds, they can be applied in complementary treatment protocols. In this study, EA whole fruit extract's cytotoxic effects were investigated on both animal and human glioblastoma cancer cell lines. The extract of whole fruit of EA was obtained by maceration in ethanol. The phytochemical compounds were identified using high-performance liquid chromatography (HPLC). The MTT and LDH assays were performed to evaluate the cytotoxicity effect of the extract and the determination of cell death. Wound-healing assay and colony formation analysis were employed for migration and proliferation evaluation. Based on HPLC analysis, the main flavonoid components of the extract were included rutin and apigenin. The results demonstrated that EA extract at the dose of 125 to 2000 µg/ml and 61.5 to 2000 µg/ml inhibited C6 and U87 cells' viability and induced significant cell cytotoxicity at both 48 and 72 h incubation times. Besides, EA extract significantly inhibited cell migration and colony formation at 250 to 1000 µg/ml concentration. Overall, the results showed that EA extract could inhibit several stages in glioblastoma carcinogenesis in vitro. Therefore, it can be suggested as an anticancer in the clinical treatment approaches of glioblastoma cancer.
BACTERIAL INHIBITORY EFFECT OF ALGERIAN POMEGRANATE (Punica Granatum L.) EXTRACTS (PEEL, JUICE, AND SEED) AGAINST MULTIDRUG RESISTANT BACTERIA
Authors: DEBIB Aicha , Menadi Soumaya , SAHNOUNI‎ Fatima , BOUKHATEM Mohamed Nadjib , OUAHCHIA Celia , KACED Amel
Published: February 2022

Punica granatum L. (pomegranate) is known popularly by its nutritional values and healthy benefits. The current study is aimed the evaluation of antibacterial effects of Punica granatum fruit extracts that cultivated in Algeria against environmental and clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamase ESBL-producing Enterobacteriaceae by using agar diffusion methods and minimum inhibitory concentration (MIC)-determination. Moreover, the extracts were investigated for their phenolic content by the Folin-Ciocalteu method. The quantitative assays of total polyphenols revealed a richness of the different pomegranate extract in polyphenols, peel and seed ethanol extracts contain the highest values (880.06 ± 11.2 mg GAE/100 g and 1160.1 ± 45.20 mg GAE/100 g, respectively). Flavonoid contents were high in both of acetone, ethanol extracts of peel, followed by ethanol, and acetone extracts of juice.  According to the disc diffusion data, all phenolic extracts had a bacterial inhibitory effect against all extended-spectrum beta-lactamase ESBL-producing Enterobacteriaceae with a strong inhibitor with MIC 512 g/mL strength. The pericarp extract is particularly effective against Escherichia coli, Citrobacter freundii, and Klebseilla pneumoniae. However, the extract Punica granatum (pericarp and juice) revealed broad-spectrum antibacterial activity with an inhibitory diameter zone size of 11 0.9 mm to 29 1.12 mm against β-lactamase generating methicillin resistant Staphylococcus aureus (MRSA) species.These results were similar in both of agar diffusion and MIC methods. From the above study, it can be concluded that Algerian pomegranate extracts possess remarkable antibacterial activity. This result could be related to the presence of bioactive metabolites in this fruit. Therefore, intensive future studies should be performed to characterize the antibacterial components that are behind for this biological property.
DEVELOPMENT AND CHARACTERIZATION OF PHYSICO-CHEMICAL AND FUNCTIONAL PROPERTIES OF GREEN TEA YOGHURT
Authors: Agarwal Aparna , Gupta Manya , Kumari Anjana , Gupta Abhishek
Published: February 2022

The present study aimed at developing a low-fat, low-sugar green tea yoghurt. The final acidities of the green tea and plain yoghurts were similar, but the lactic acid count in green tea yoghurt (5.8*107 cfu/g) was twice of that in the plain yoghurt (2.7*107 cfu/g). This suggested that the tea extract buffered pH lowering during fermentation. Sensorially, the green tea yoghurt was at par acceptable with the plain yoghurt. The total phenolic content of green tea yoghurt was significantly higher (397.9 µg GAE/ mL) than that of the plain yoghurt (81.0 µg GAE/ mL). These polyphenols have proven health benefits like gut health, weight loss, and bone health.
MONITORING OF ORGANIC VOLATILE COMPOUNDS IN CRAFT BEERS DURING FERMENTATIVE PROCESS
Authors: Gonçalves Bortoleto Gisele , Pinheiro Claro Gomes Winston
Published: February 2022

The present study aimed to evaluate the formation of volatile organic compounds (VOCs) during the fermentation process of three different craft beers, using gas chromatography with headspace sampling. The production of VOCs, which are largely responsible for the flavors of the drink, makes up unique organoleptic characteristics for each beer. In this work, it was possible to observe the different profiles for the Pilsen, Witbier and IPA style beers, when quantifying the analytes methanol, ethanol, n-propanol, iso-butanol, 3-methyl-1-butanol (iso-amyl alcohol), acetaldehyde, ethyl acetate and iso-amyl acetate. Pilsen beer, with low fermentation, presented the ethanol content in the same concentration range as Witbier beer, as expected, but with higher alcohols and lower esters than the other two. Witbier and IPA beers, both of high fermentation, presented isoamyl alcohol concentration above expectations, while IPA beer, present higher concentrations of n-propanol, ethyl acetate and isoamyl acetate in relation to Pilsen and Witibier, which is related to the complexity of its recipe.
GENOME-PLASMON INTERACTION EFFECT ON RESISTANCE TO SEPTORIOSIS IN WINTER TRITICUM AESTIVUM L ALLOPLASMATIC LINES
Authors: Li Yonchao , Vlasenko Volodymyr , Bakumenko Olha , Kovalenko Ihor , Bilokopytov Vasyl , Li Dongxiao , Li Gan
Published: February 2022

The results of studies on the resistance control to Septoria tritici with alloplasmic lines of bread winter wheat with 14 cytoplasms. The effect of the Triticum aestivum L nuclear genome on its interactions with the cytoplasms of related species on wheat resistance was found out. Analyzing the obtained resistance evaluation results of the genotypes involved in the pathogen Septoria tritici experiment showed the absence of immune forms under the conditions of the Forest-Steppe of Ukraine. The affection intensity of septoria within the control variants over the years was relatively equal from score 5.0 to 4.0 (average - 4.43). Analyzing the nuclear-plasmon effect and genome-plasmon interaction for resistance to septoria, we found out that different sources of alloplasm and the nuclear genome interact differently and provide different resistance effects to septoria. Two nuclear genomes – Donskaia Poluintensivaia and Myronivs’ka Yuvileina were identified with the highest score (5.0). On average, over the years of study, statistically significant data on the response of different plasmotypes to the septoria phytopathogen elicited high variability in both plasmon and genome. The proportion of the genome (p-level - 4.97-09) compared with plasmon (p-level - 1.35-05), in the trait variability was almost twice as large. Evaluation of the variability level (according to the results of the sum of squares allocation) caused by the study showed that the factor B input (nuclear genome) was 10.7%, and the factor A input (cytoplasm) was 28.1%. The interaction of nuclear and cytoplasmic factors accounts 12.1%, and the share of random deviations - 49.1%.
WGS CHARACTERIZATION OF ENTEROCOCCUS FAECALIS H1041 ISOLATED FROM THE TRADITIONAL BULGARIAN GREEN CHEESE
Authors: Dimov Svetoslav , Georgieva-Miteva Dimitrina , Dekova Tereza , Kitanova Meglena , Peykov Slavil , Strateva Tanya
Published: February 2022

In this study, Enterococcus faecalis H1041 which was isolated from the traditional Bulgarian green cheese was characterized in depth by a whole-genome sequencing performed on the Illumina HiSeq platform using 2 x 150 bases paired-end reads mode. The bioinformatic analysis performed only with free online tools revealed that the strain resembles other cheese-born E. faecalis strains by its antibiotics’ resistances and virulence genotype, thus its presence within the green cheese should not be considered as a health risk. Only two antibiotic resistance genetic determinants were found, but they both carry mutations, and probably are not functional. Furthermore, 18 virulence factor genetic determinants were found, but only 6 of them possessed 100% identities with the canonical sequences, and being more or less intrinsic for the Enterococcus genus. None of them were found on contigs that contain sequences with known plasmid origin of replication. Enterolysin A gene was also detected which most probably plays role in ripening process of the Bulgarian green cheese.
PREVENTIVE EFFECTS OF CITRULLUS COLOCYNTHIS. L PLANT EXTRACT ON DELTAMETHRIN PESTICIDE INDUCED PNEUMOTOXICITY IN WISTER RATS
Authors: Gasmi Salim , Benaicha Brahim , Kebieche Mohamed , Mennai Imad
Published: February 2022

Pesticides are chemicals used to fight agricultural pests. Their use causes extensive damage to both humans and animals, resulting in diseases mainly affecting the respiratory and digestive systems. Through this study, we evaluated the pneumotoxicity caused by a deltamethrin (DM) insecticide at a dose of 0.64mg.kg-1.day-1. We also evaluated the preventive and curative effect of the medicinal plant "citrullus colocynthis. L" antagonist of this toxicity at a dose of 150mg.kg-1.day-1. We carried out the experimental application on Wister rats as an animal model of exhalation. Several experiments were carried out on the activity of enzymes and vital indicators. This study revealed a decrease in the concentration of the protein accompanied by a decrease in the means of development of the body [25±0.23]. Then, our study showed that DM has an oxidative effect, resulting in a decrease in the level of GSH [0.05 ±0.00] and the enzymatic activity of GPx [0.34±0.05]. In addition to an increase in the level of MDA [4.90±0.72] and the enzymatic activity of GST [0.84±0.11]. In addition, the histological study carried out on a fragment of lung of rats treated with this pesticide showed changes in the histological structure of the lungs resulting in vascular congestion, infiltration of inflammatory cells, thickening of the walls of blood vessels, as we do. Observed intra-alveolar hemorrhages and hyperplasia of the alveolar lining. On the other hand, we have confirmed the preventive effect of the citrullus colocynthis. L after treatment with this pesticide, which has an anti-oxidant activity, which can reduce the intensity of the oxidative stress resulting from deltamethrin and its effectiveness. In the treatment of lung, tissue effected by this pesticide and it is stored in its natural state.
FERMENTABLE OLIGOSACCHARIDES, DISACCHARIDES, MONOSACCHARIDES AND POLYOLS AND THEIR ROLE IN FOOD DIGESTION
Authors: Urminská Dana , Haring Nora , Fábry Vladimír , Urminská Jana
Published: February 2022

Oligosaccharides containing fructose (fructans, inulin), galactose (galactans, stachyose, raffinose), disaccharides enclosing galactose (lactose), monosaccharides and mixtures of monosaccharides encompassing fructose (fruit sugar, honey) and saccharide polyols (mannitol, malbitol, sorbitol), are considered as safe to be consumed and beneficial to health. Gradually, however, their presence in the human diet reaches amounts that are indigestible by the small intestine, causing an osmotic load on the intestinal lumen. Subsequently, they pass into the large intestine, where they are fermented by intestinal bacteria to undesirable products such as gaseous carbon dioxide, hydrogen, methane, sulfane, and short chain fatty acids. Indigestible fermentable oligosaccharides, disaccharides, monosaccharides, and polyols may cause undesirable symptoms such as an increased flatulence, bloating, abdominal pain, alterations to the intestinal physiology, cessation of peristalsis, or diarrhea, which are manifested by a wide array of diseases of the gastrointestinal tract, such as the irritable bowel syndrome or small intestinal bacterial overgrowth. Fruits, vegetables, dairy products, legumes, some cereals, as well as honey or various sweeteners are among the most common food sources of these carbohydrates.
Analysis and Development of Education and Training Management Information Systems at the Jambi Province Human Resources Development Agency
Authors: Bramana Jaya Safutra
Published: February 2022

The Jambi Province Human Resources Development Agency has created a training information system in an effort to manage human resource management in regencies, cities and throughout the province of Jambi. However, along with the implementation of the use of this training system, several problems were still found in the use of the system, both from the apparatus in the BPSDM agency and the apparatus as training participants. There are some obstacles to the implementation of the system, such as the lack of consistency of the organizing committee in using the education information system on the grounds that it is still difficult to understand the interface pattern in the application system, so the committee is reluctant to input data and use the application system as a registration facility for training participants. So in the implementation of activities, there are often errors in data and information of training participants which result in errors in the training implementation report. To solve this problem, the researchers conducted an analysis and development of the education management information system at the Jambi Province Human Resources Development Agency with the aim of making users it easier forto easily understand the use of these web-based applications.
Participants' Perceptions of the Implementation of Basic Training (LAatsar) for Civil Servants with the Blended Learning Model in Batanghari Regency in 2021
Authors: Musfarita Affiani
Published: February 2022

One of the program activities of BPSDM Jambi Province which is carried out every year is a training program for retired personnel, the aim is to prepare a number of alternative activities for officials who will enter retirement. This research is about the implementation process of retired entrepreneurial training. This research is a qualitative descriptive study, the informants in this study were the Head of the Jambi Province Human Resources Development Agency, the Head of the Core Technical Competency Development Division, the Heads of Sub-Heads, and the organizing committee. Data were obtained through interviews and activity report books and observations. The data analysis method is to use the data triangulation method, namely by making comparisons and discussions between the data obtained in the field. This research resulted that the implementation of entrepreneurial training Pra Purnabakti at BPSDM Jambi Province has been going well and quite systematically. The implementation process is divided into two parts, namely the planning process and the implementation process. This training planning activity is divided into two parts of planning, namely program planning, and implementation planning. Program planning consists of analyzing the needs of prospective participants of the Retired Entrepreneurship Training and compiling a module for the Retired Entrepreneurship Training program. Meanwhile, the planning for the implementation consists of determining the partners for the implementation of the training, the setting of the place, and the time of the implementation. For more success in the implementation of the training, it is hoped that the BPSDM will budget an adequate budget, due to the implementation of activities in the field.
In vitro rooting of Nepenthes truncata Macf.
Authors: Rasco Eufemio T. , Oguis Georgianna Kae R. , Silvosa Cyrose Suzie C.
Published: February 2022

Rooting is an important stage in propagation of in vitro-grown delicate plants such as Nepenthes truncata. With well-developed root systems, plants are hardy enough to withstand changes in the environment during transfer from laboratory to nursery, thereby ensuring high survival rates.
Many triggerplants (Stylidium spp.; Stylidiaceae) form arbuscular mycorrhizal associations
Authors: Moberly Stephen P. , Watson Maxine , Darnowski Douglas W.
Published: February 2022

Triggerplants (Stylidium spp.; Stylidiaceae) grow in Australia and a few areas to its north, though >95% of the >300 species in the genus Stylidium are endemic to Australia. Their name comes from the extremely rapid, active, and resettable pollination mechanism which they use. In addition, they have been recently recognized as possessing at least some of the traits of carnivorous plants, specifically the ability to trap and digest prey insects, as well as the more recently-demonstrated ability to transport and, therefore, benefit, from nutrients obtained in that way. Generally, they trap prey which are much smaller than their pollinators. Triggerplants typically are found in extremely nutrient poor conditions where genera such as Drosera and Utricularia are usually found in the same places.
Prey capture by Dionaea muscipula A review of scientific literature with supplementary original research
Authors: Williams Stephen E. , Hartmeyer Siegfried R. H.
Published: February 2022

Many descriptions of prey capture by Dionaea muscipula (Venus flytrap) in popular publications and educational literature are inaccurate. Here we review well documented literature on prey capture in this plant’s natural habitat and add observations on prey capture and attraction mechanisms we have observed in plants cultivated in a greenhouse and garden.
Variations of a Story: A Narrative and Literary Comparison of Johann Peter Hebel’s Unexpected Meeting and E.T.A. Hoffmann’s The Mines of Falun
Authors: Scullion Val
Published: February 2022

This article, through close textual analysis, compares the oral and literary dynamics of two narrative pieces: Johann Peter Hebel’s Unexpected Meeting (1811) and E.T.A. Hoffmann’s The Mines of Falun (1819). Against a background of their almost coterminous birth and death dates, respectively 1766-1826 and 1776-1822, and the close publication dates of these narratives, the line of argument explores the individual approaches of Hebel and Hoffmann as they flesh out the same story in completely different ways. It argues that both authors follow their own aesthetic principles, the former influenced by Enlightenment values and the latter mediating the preoccupations of German Romanticism.
On the Sustainability of Fiscal Policy in Sierra Leone
Authors: Bonzu Samuel
Published: February 2022

The aim of this paper is to empirically investigates whether Sierra Leone fiscal policy is sustainability. In this regard, I employ different econometric methodologies used in the empirical literature to investigates the sustainability of fiscal policy. The empirical findings that emerged from this study are useful on one hand to creditors, serving as a guide for lending to the government, and, on the other hand to the government, cautioning policymakers to avoid public debt from exploding that could possibly lead to fiscal insolvency and/or debt distress. I start by testing for the stationarity properties of the primary balance, the necessary condition for a sustainable fiscal policy. The findings indicated that Sierra Leone fiscal policy is sustainable under the review period. Next, I test for cointegration relationship between government revenue and government expenditure, the alternative approach to test for a sustainable fiscal policy. On this note, I employ both the Dynamic Ordinary Least Square (DOLS) and the Johansen cointegration techniques. Both approaches confirmed the existence of a cointegration relationship between government revenue and government expenditure. The estimated cointegration coefficients show that fiscal policy during the review period is weakly sustainable and the cointegration between government spending and revenue is positive (but less than one) and statistically significant. This implies that for each percentage point of GDP increase in government expenditure, government revenues increase by less than one percentage point of GDP. Additionally, I proceeded to endogenously account for structural breaks in the cointegration relationship, which is relevant for Sierra Leone, a country that has witnessed significant changes over the years, including the Structural Adjustments Programme (SAP) in the 1980s, tax reforms in the 1990s and 2000s, etc. I found evidence of a significant structural break occurring in 1984. There also exists uni-directional causality running from government revenue to government expenditure. This causality result is in line with the tax-and-spend hypothesis as proposed by Friedman (1978).Finally, I estimate an error correction model and the error correction term shows that the speed of adjustment from the expenditure side works faster than that of the revenue side to correct the fiscal disequilibrium.
PERFORMA BETON ABU CANGKANG TELUR SEBAGAI BAHAN TAMBAH SEMEN
Authors:   Mahasiswa Manajemen Rekayasa Konstruksi, Teknik Sipil, Politeknik Negeri Malang, Abidin Mochamad Nur , Qomariah  ,   Dosen Jurusan Teknik Sipil, Politeknik Negeri Malang, Naibaho Armin ,   Dosen Jurusan Teknik Sipil, Politeknik Negeri Malang
Published: February 2022

Dunia konstruksi telah mengalami kemajuan yang sangat pesat. Semakin tingginya kebutuhan bahan pembangunan infrastruktur, penyerapan energi untuk mendukung aktivitas tersebut tentunya akan semakin besar.Untuk sebuah negara seperti Indonesia, oleh karena itu, perlu adanya alternatif untuk memanfaatkan limbah yang terbuang seperti cangkang telur.Dengan jumlah limbah cangkang telur yang banyak dengan menggilingnya bisa digunakan sebagai pengganti sebagian atau keseluruhan semen.Penelitian ini bertujuan menganalisis beton subtitusi abu cangkang telur terhadap semen tinjauan kuat tarik belah dan kuat tekan.Metode penelitian ini menggunakan perancangan campuran beton menggunakan SNI 03-2834-2000.Sampel benda uji setiap variasi 0%, 5%, 7,5%, dan 10% berjumlah 8 benda uji silinder untuk pengujian kuat tarik belah dan 72 benda uji silinder untuk pengujian kuat tekan. Hasil pengujian kuat tarik belah umur 28 hari secara berturut-turut untuk variasi 0%, 5%, 7,5%, dan 10% sebesar 13,33 kg/cm2; 12,98kg/cm2; 10,18 kg/cm2; dan 10,33 kg/cm2. Hasil pengujian kuat tekan umur 28 hari secara berturut-turut untuk variasi 0%,5%, 7,5%, dan 10% sebesar 37,97 kg/cm2; 36,59 kg/cm2; 22,63 kg/cm2; dan 28,64 kg/cm2. Biaya rata-rata pengujian secara berturut-turut untuk variasi 0%, 5%, 7,5%, dan 10% sebesar Rp 1,040.217,00/m3; Rp 1.163.669,00/m3; Rp 1.225.398,67/m3; dan Rp 1.287.125,9/m3. Substitusi abu cangkang telur menurunkan nilai kuat tarik belah rata-rata, nilai kuat tekan rata-rata dan semakin banyak subtitusi abu cangkang telur terhadap semen maka pengeluaran biaya anggaran semakin tinggi.
PENGARUH PENAMBAHAN SIKACIM PADA CAMPURAN BETON POROUS UNTUK PERKERASAN JALAN TERHADAP KUAT GESER DAN INFILTRASI
Authors:   Mahasiswa Manajemen Rekayasa Konstruksi, Jurusan Teknik Sipil, Politeknik Negeri Malang, Ramadhani Dinda Awalia , Marjono  ,   Dosen Jurusan Teknik Sipil, Politeknik Negeri Malang, Qomariah  ,   Dosen Jurusan Teknik Sipil, Politeknik Negeri Malang
Published: February 2022

Aplikasi beton porous atau beton berpori untuk perkerasan jalan semakin mendapat perhatian di bidang Teknik Sipil sebagai solusi alternatif karena mampu mengurangi limpasan air hujan serta mencegah banjir. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan SikaCim Bonding Adhesive terhadap karakteristik beton porous berdasarkan kuat geser yang didapat dari pengujian kuat tarik belah dan infiltrasi beton porous dengan campuran normal komposisi semen:agregat 1:5 dan FAS = 0,5. Pengujian kuat geser dan infiltrasi dilakukan pada beton porous umur 21 dan 28 hari. Hasil penelitian kuat geser pada beton porous rata-rata tertinggi dicapai pada variasi 1:1,5 pada umur 28 hari sebesar 1,604 Mpa dengan persamaan Y= -6.7401x² + 16.999x - 9.145. Y = Kuat Geser (Mpa), X = Beban Geser (kN). Penelitian Infiltrasi dengan penambahan variasi SikaCim 1:1,5 pada beton porous diperoleh sebesar 0,123 lt/dt pada umur 28 hari dengan persamaan Y= 8.4975x² - 18.582x + 10.225. Y = Infiltrasi (lt/dt), X = waktu aliran (dt). Hal ini dapat disimpulkan bahwa penambahan SikaCim memiliki pengaruh dalam meningkatkan ikatan antara agregat dan pasta semen sehingga memperbaiki karakteristik Kuat Geser Beton Porous dan meningkatkan Infiltrasinya. Biaya Konstruksi yang diperlukan adalah Rp 2.622.006,-
STRATEGI DAN METODE PELAKSANAAN PEKERJAAN STRUKTUR ATAS PROYEK APARTEMEN ADHIGRYA PANGESTU MARGONDA RAYA DEPOK
Authors:   Mahasiswa Manajemen Rekayasa Konstruksi, Jurusan Teknik Sipil, Politeknik Negeri Malang, Afandi Muhammad Zulfikri , Khamim Moch. ,   Dosen Jurusan Teknik Sipil, Politeknik Negeri Malang, Naibaho Armin ,   Dosen Jurusan Teknik Sipil, Politeknik Negeri Malang
Published: February 2022

Proyek pembangunan Apartemen Adhigrya Pangestu Margonda Raya Kota Depok memiliki luas bangunan 30.200 m2 berada di atas lahan seluas 4.500 m2 dan memiliki kontrak 500 hari kalender. Proyek high rise building ini terletak di tengah Kota Depok dan terdiri dari 22 lapis lantai. Pekerjaan struktur merupakan salah satu pekerjaan yang paling utama dalam sebuah proyek pembangunan high rise building, dengan demikian perencanaan strategi dan metode pelaksanaan yang tepat akan menunjang proses pekerjaan sehingga dapat berjalan sesuai dengan target waktu, biaya, dan mutu yang telah disyaratkan. Perencanaan strategi dan metode pelaksanaan bertujuan untuk mengetahui bagaimana suatu proyek dilaksanakan dengan pembuatan langkah–langkah berupa flowchart tahap pelaksanaan yang disertai dengan deskripsi dan diilustrasikan menggunakan gambar visual rencana sehingga dapat mengetahui kebutuhan komponen proyek berupa alat, material, dan tenaga kerja yang akan dipakai secara sequence untuk setiap pekerjaan. Item pekerjaan struktur pada proyek Apartemen Adhigrya Pangestu ini adalah pekerjaan kolom, balok, pelat, shear wall, dan tangga. Dari hasil studi, jam kerja pada proyek ini direncanakan selama 12 jam dengan rincian pekerjaan struktur dibagi menjadi dua zona kerja berdasarkan kapasitas layanan alat berat yaitu tower crane. Durasi pelaksanaan direncanakan 420 hari kerja dengan total anggaran biaya pelaksanaan sebesar Rp. 137.702.963.000,00.
Indefinite determiners in two northern Italian dialects
Authors: Lebani Gianluca E. , Giusti Giuliana
Published: February 2022

Italian and Italian dialects express indefiniteness in different ways, among which with a null determiner (ZERO) like all other Romance languages, but also with the definite article (ART) unlike what is found in Romance. Italian and some northern Italian dialects also display the so-called “partitive determiner” DI+ART, which is present in French. Few northwestern Italian dialects display (bare) DI, parallel to French. We adopt Cardinaletti and Giusti’s (2015, 2016) unified analysis and build on Cardinaletti and Giusti’s (2018, 2020) hypothesis that the variation and optionality in the distribution of the four determiners in regional Italian mirror their distribution in Italian dialects along two isoglosses: the ART isogloss spreading from the center of Italy towards north-west and south-east; and the DI isogloss spreading from Piedmont eastwards. We conduct a quantitative analysis on the results of a questionnaire in Piacentino and Rodigino. We test the distribution of the four determiners with mass and count nouns in two dimensions: sentence type (positive vs. negative) and predicate type (telic vs. atelic). The results confirm the hypothesis that the complexity of the determiner is related to its distribution highlighting two hierarchies of contexts: NEG < POS and ATEL < TEL. It also confirms that Piacentino, located at the crossroads of the ART and DI isoglosses, has more optionality than Rodigino, located at their borders.
Interpretive asymmetries between null and overt PRO in complement and adjunct infinitives in (Colombian) Spanish
Authors: Gómez Kryzzya , Duguine Maia , Demirdache Hamida
Published: February 2022

Cross-linguistically, control complement clauses have been reported to allow overt pronominal subjects displaying the diagnostic properties of obligatory control (‘Overt PRO’; see Livitz (2011) and reference therein). Building on Gómez (2017), we extend the empirical range of the overt PRO phenomena to para finality adjunct clauses in (Colombian) Spanish. We show that the controlled subject of para-infinitives ―be it null PRO or overt PRO― has the same distribution and interpretive properties as that of complement infinitives. We bring to light unexpected asymmetries in the interpretive properties of overt vs. null PROs which we dub the Overt vs. covert PRO paradox: while they both only allow a bound variable reading under ellipsis, overt PRO, unlike null PRO, also allows a coreferential reading under association-with-focus. Here again, the data are identical in complement vs. adjunct control. We account for this paradox by putting forth the Anaphor Generalizations, which state that (i) both overt and null anaphors must be syntactically bound, and (ii) while null anaphors must be semantically bound, overt anaphors can but need not be semantically bound. We further show how the Anaphor Generalizations can be extended to account for similar patterns of interpretation reported for English and French reflexives.
Differences in Biochar Sources for Controlled Nitrogen Loss in a Hybrid Maize Agroforestry System with Melaleuca cajuputi
Authors: Kastono Dody , Suryanto Priyono , Rogomulyo Rohlan , Handayani Suci , Supriyanta Supriyanta , Habib Widyawan Muhammad , Alam Taufan
Published: February 2022

Biochar is used to improve soil fertility and control nitrogen loss in soil. This study aimed to evaluate the difference between biochar sources, namely Melaleuca cajuputi waste and rice husk, for controlled nitrogen loss in hybrid maize planted between Melaleuca cajuputi stands. A split-plot design with three replications was used. The main plot was composed of biochar sources (BS), i.e., without biochar application (WB), Melaleuca cajuputi biochar (MCB), and rice husk biochar (RHB). The subplot was the urea fertilizer dosage, i.e., 0, 150, 300, and 450 kg/ha. The observation parameters were nitrate reductase activity (NRA), total chlorophyll (TC), leaf photosynthesis rate (LPR), nitrogen loss (NL), nitrogen use efficiency (NUE), and seed yield per hectare (SY). The data were analyzed with ANCOVA and LS-means. The results showed that there was no significant difference between mixing MCB or RHB in UF for all hybrid maize parameters, whereas significant differences were observed with WB. The NL values ​​of MCB and RHB were 13.85 and 13.08 kg/ha N, i.e., NL was significantly reduced by 70.90% and 72.51%, and the percentage of SY increased by 28.60% and 37.94% compared to WB, respectively.
Pengaruh Konsumsi Ubi Cilembu Terhadap Penurunan Kadar Asam Laktat Pada Atlet Rugby
Authors: Syafriani Rini , Ayustin Yufiany , Bahri Samsul , Ramania Nia Sri , Winata Bagus
Published: February 2022

Dalam ubi terdapat beberapa kandungan Polyphenols yang dapat bermanfaat bagi sistem metabolisme tubuh. Oleh karena itu, tujuan daripada penelitian ini adalah untuk mengukur efektivitas konsumsi ubi pada penurunan asam laktat atlet rugby. Total 30 peserta dibagi ke dalam 3 group, yaitu: Group 1 berisikan (n = 10) yang mengonsumsi ubi cilembu, Group 2 berisikan (n = 10) yang mengonsumsi roti tawar, dan Group 3 (n = 10) yang mengonsumsi hanya air mineral saja. Seluruh peserta melakukan pengukuran anthropometry sebelum melakukan pengujian. Pengukuran Pre-test dan Post-test meliputi pengukuran asam laktat sebelum dan sesudah pertandingan rugby. Seluruh atlet diwajibkan untuk mengonsumsi sesuai dengan tugas yang diberikan oleh setiap group selama 7 hari lamanya. Hasil statistik menunjukkan bahwa hanya pada group 1 (konsumsi ubi cilembu) yang memiliki penurunan asam laktat yang signifikan antara pre-test dan post-test (p = 0.04), Sedangkan tidak ada perbedaan signifikan pada group 2 (p = 0.07) dan group 3 (p = 0.08) pada laktat setelah pertandingan. Penelitian ini menunjukkan bahwa konsumsi ubi Ipomoea batatas (L.) Lam yang berasal dari cilembu, dapat menurunkan kadar asam laktat setelah selesai bertanding lebih cepat dibandingkan dengan konsumsi roti tawar.