bluuebunny/crossref_metadata_2025 huggingface dataset:
The influence of signs of social class on compassionate responses to people in need
Authors: Callaghan Bennett , Delgadillo Quinton M. , Kraus Michael W.
Published: August 2022

A field experiment (N = 4,536) examined how signs of social class influence compassionate responses to those in need. Pedestrians in two major cities in the United States were exposed to a confederate wearing symbols of relatively high or low social class who was requesting money to help the homeless. Compassionate responding was assessed by measuring the donation amount of the pedestrians walking past the target. Pedestrians gave more than twice (2.55 times) as much money to the confederate wearing higher-class symbols than they did to the one wearing lower-class symbols. A follow-up study (N = 504) exposed participants to images of the target wearing the same higher- or lower-class symbols and examined the antecedents of compassionate responding. Consistent with theorizing, higher-class symbols elicited perceptions of elevated competence, trustworthiness, similarity to the self, and perceived humanity compared to lower-class symbols. These results indicate that visible signs of social class influence judgments of others’ traits and attributes, as well as in decisions to respond compassionately to the needs of those who are suffering.
Intervention effect of group counseling on social support and post-stress growth of orphans and vulnerable children in China
Authors: Zhang Lyuci , Wu Sumei , Roslan Samsilah , Zaremohzzabieh Zeinab , Chen Ye , Jiang Yuqin
Published: August 2022

Orphans and vulnerable children fall under the category of children who are at risk of exposure to more stressful circumstances and receive less social assistance compared to other children. This study aims to investigate the impact of group counseling based on social intervention and psychological therapy (SSGPC) on social support and the perceived stress growth of orphans and vulnerable children. In one special educational needs school in Nanning, China, the SSGPC was developed and implemented. Using the social support and post-stress growth scales, the researchers investigated the effects of SSGPC on orphans and vulnerable children. Twenty-seven orphans and vulnerable children between the ages of nine and 12 were arbitrarily assigned to the experimental and control groups. A pre-test post-test method of quasi-experimental design was applied, with 13 participants in the experimental group and 14 in the control group. The results revealed that the intervention group had significantly higher scores for social support and post-stress growth than the control group. The SSGPC had significantly improved the levels of social support for orphans. The findings indicated that the SSGPC provided an effective way to improve social support and post-stress growth of orphans and vulnerable children.
In search of well-established evaluative criteria for the emerging qualitative methods of L2 affective variables
Authors: Du Jiayi
Published: August 2022

Qualitative research is marked by context sensitivity with an inclusion of the participants’ points of view and setting, how they can affect how participants feel and how this feeling is captured and interpreted by researchers (Yardley, 2017). This is the value of exploring affective variables through qualitative research. The present study focuses on the qualitative studies of L2 affective variables in recent years led by the complexity dynamic systems theory (CDST). This new line of research has employed innovative research methods compatible with the CDST, and has had useful findings. Yet, they seem to lack rigor and systematicity of research. Thus, this observed lack of consistency is problematized in the present study and attempts are made to set evaluative criteria for the judgment of the burgeoning studies and guiding the future line of qualitative and dynamic inquiry in the L2 affective domain. To this aim, the different sets of evaluative criteria proposed for qualitative research are derived from the mostly cited scholars in the research methodology. The relevance of each to the dynamic qualitative investigations of L2 affective variables is discussed. Then these evaluative criteria are abstracted and put forth for the qualitative research in the dynamic phase of second language acquisition (SLA) research. The overall goal is to guide future researchers with an interest in investigating the dynamic and developmental nature of L2 affective variables qualitatively. These evaluative criteria can pave the way for the emergence of more rigorous and systematic qualitative studies of L2 affective factors in the future.
Factoring and correlation in sleep, fatigue and mental workload of clinical first-line nurses in the post-pandemic era of COVID-19: A multi-center cross-sectional study
Authors: Liu Yan , Xian Ji Shu , Wang Rui , Ma Kang , Li Fei , Wang Fei Long , Yang Xue , Mu Ning , Xu Kai , Quan Yu Lian , Wang Shi , Lai Ying , Yang Chuan Yan , Li Teng , Zhang Yanchun , Tan Binbin , Feng Hua , Chen Tu Nan , Wang Li Hua
Published: August 2022

Background

A better understanding of the factors and their correlation with clinical first-line nurses’ sleep, fatigue and mental workload is of great significance to personnel scheduling strategies and rapid responses to anti-pandemic tasks in the post-COVID-19 pandemic era.

Objective

This multicenter and cross-sectional study aimed to investigate the nurses’ sleep, fatigue and mental workload and contributing factors to each, and to determine the correlation among them.

Methods

A total of 1,004 eligible nurses (46 males, 958 females) from three tertiary hospitals participated in this cluster sampling survey. The Questionnaire Star online tool was used to collect the sociodemographic and study target data: Sleep quality, fatigue, and mental workload. Multi-statistical methods were used for data analysis using SPSS 25.0 and Amos 21.0.

Results

The average sleep quality score was 10.545 ± 3.399 (insomnia prevalence: 80.2%); the average fatigue score was 55.81 ± 10.405 (fatigue prevalence: 100%); and the weighted mental workload score was 56.772 ± 17.26. Poor sleep was associated with mental workload (r = 0.303, P < 0.05) and fatigue (r = 0.727, P < 0.01). Fatigue was associated with mental workload (r = 0.321, P < 0.05). COVID-19 has caused both fatigue and mental workload. As 49% of nurses claimed their mental workload has been severely affected by COVID-19, while it has done slight harm to 68.9% of nurses’ sleep quality.

Conclusion

In the post-COVID-19 pandemic era, the high prevalence of sleep disorders and fatigue emphasizes the importance of paying enough attention to the mental health of nurses in first-class tertiary hospitals. Efficient nursing strategies should focus on the interaction of sleep, fatigue and mental workload in clinical nurses. In that case, further research on solutions to the phenomenon stated above proves to be of great significance and necessity.

Clinical trial registration

[https://clinicaltrials.gov/], identifier [ChiCTR2100053133].
Medical-legal partnerships: An integrated approach to advance health equity and improve health outcomes for people living with HIV
Authors: Martinez Omar , Munoz-Laboy Miguel , Davison Robin
Published: August 2022

Medical Legal Partnerships (MLPs) offer a structural integrated intervention that could facilitate improvements in medical and psychosocial outcomes among people living with HIV (PLWH). Through legal aid, MLPs can ensure that patients are able to access HIV services in a culturally sensitive environment. We conducted organizational-level qualitative research rooted in grounded theory, consisting of key informant interviews with MLP providers (n = 19) and members of the Scientific Collaborative Board (SCB; n = 4), site visits to agencies with MLPs (n = 3), and meetings (n = 4) with members of the SCB. Four common themes were identified: (1) availability and accessibility of legal and social services support suggest improvements in health outcomes for PLWH; (2) observations and experiences reveal that MLPs have a positive impact on PLWH; (3) 3 intersecting continua of care exist within MLPs: HIV care continuum; legal continuum of care; and social services continuum; and (4) engagement in care through an MLP increases patient engagement and community participation. The MLP approach as a structural intervention has the potential to alleviate barriers to HIV/AIDS treatment and care and thus dramatically improve health outcomes among PLWH.
Parent perspectives about pediatric urology telemedicine visits before and after COVID-19-driven rapid implementation
Authors: Walton Ryan F. , Arora Hans C. , Berkowitz Rachel J. , Rosoklija Ilina , Cheng Earl Y. , Johnson Emilie K.
Published: August 2022

Research Objective

Telemedicine is now a critical healthcare delivery modality. Prior studies of telemedicine in pediatric urology are lacking parent/patient perspectives. This study aims to assess interest in and factors associated with willingness to participate in telemedicine, and to compare perceptions of telemedicine before and after pandemic-related implementation.

Study Design

This was a cross-sectional study of parents of pediatric urology patients that attended in-person clinic visits (Pre-Telemedicine cohort; January-March 2020) and telemedicine visits (Post-Telemedicine cohort; April-July 2020).

Principal Findings

The response rates were 83.4% (Pre-Telemedicine, N =205) and 32.5% (Post-Telemedicine, N =89). Overall, most parents had a college or graduate level degree (71.9%), private insurance (71.6%), and a plurality were 36-40 years old (30.7%). Their children were mostly white (68.9%), 23.7% were Hispanic/Latino, and the median patient age was 3.3 years (range: 0.1–32.1). There were no demographic differences between the Pre-and-Post-Telemedicine sub-groups.

Pre-Telemedicine

Most (84.3%) were willing to participate in a new, follow-up, or post-operative telemedicine visit. There were no associations between demographics and willingness. Comfort in video visits without a physical exam (3.23% Unwilling vs 38.2% Willing, = <0.001), comfort with technology (22.6% vs 71.6%, p = <0.001), belief that quality of care is similar between telemedicine and in-person visits (3.23% vs 24.0%, p = <0.001), and estimated costs from in-person visits (6.67% vs 30.3%, p = 0.004) were all positively associated with willingness.

Post-Telemedicine

Most indicated that telemedicine met their needs (88.6%), and that they were satisfied with the both the quality of technology used (83%) and the interaction with the urologist (89.9%) during the telemedicine visit.

Comparison of Pre-and-Post-Telemedicine

In reference to the Pre-Telemedicine respondents, Post-Telemedicine respondents more frequently strongly agreed that telemedicine is as private (51.5% vs 77.5%, p = <0.001), secure (49.5% vs 66.3%, p = 0.02), and complete (33.2% vs 51.1%, p = 0.02) as in-person visits.

Conclusions

Most parents had high willingness to participate and positive perceptions of telemedicine prior to the COVID-19 era. Factors associated with willingness were elucidated. Families that participated in telemedicine reduced direct costs and saved time. Lastly, these results suggest that confidence in telemedicine had increased after experience with telemedicine. These data support continued telemedicine access and coverage/reimbursement beyond the pandemic.
Primary breast lymphoma in males: Incidence, demographics, prognostic factors, survival, and comparisons with females
Authors: Zhang Jie , Ma Binbin , Ji Hong , Guo Rong
Published: August 2022

Background

Primary breast lymphoma (PBL) is a rare disease condition and is mainly observed in females. No male PBL cohorts were reported previously. This study aims to investigate the incidence, clinical characteristics, prognostic factors, and survival outcomes among male PBL patients and also to perform comparisons between males and females.

Methods

Patients diagnosed with PBL between 2000 and 2019 from the Surveillance, Epidemiology, and End Results (SEER) database were identified. Age-adjusted incidence rates were calculated by year and age for trend analysis. Univariate and multivariate Cox hazard proportional regression analyses were performed to identify prognostic factors. Survival comparisons were conducted using the Kaplan–Meier method and the log-rank test. The propensity matching score (PSM) method was used to balance demographics.

Results

The incidence rate of 122 male PBL patients diagnosed in the period of 2000 to 2019 was 0.169 (95% CI: 0.140–2.203) per million persons, which was much lower than that of 2,543 females (1.59, 95% CI: 1.53–1.65). Clinical demographics were similar between females and males, except for lymphoma subtype distribution (P = 0.025). A higher age [hazard ratio (HR) = 1.08, 95% CI: 1.05–1.12, P < 0.001] and not receiving radiotherapy (receiving vs. not receiving: HR = 0.41, 95% CI: 0.21–0.78, P = 0.007) were significant risk factors associated with overall survival (OS) in males. Radiotherapy (OS: P = 0.023) can offer benefits in OS. Using the PSM method, we also revealed that male PBL patients had significantly worse OS and cancer-specific survival rates than females.

Conclusions

This study first analyzed male patients with PBL involving incidence, clinical characteristics, and survival data. Sex disparity was also observed in the survival outcome of the disease.
Case report: Infratentorial Embryonal Tumor with Abundant Neuropil and True Rosettes (ETANTR) in an 8-month-old Maine Coon
Authors: Foiani Greta , Mandara Maria Teresa , Carminato Antonio , Melchiotti Erica , Corrò Michela , Vascellari Marta
Published: August 2022

An 8-month-old female Main Coon with a history of recurrent behavioral changes and anorexia was presented with sternal recumbency and depression. Within 5 days, the cat progressively worsened with symptoms of stupor and coma and was euthanized. At post-mortem examination, a solid, grayish infratentorial mass located in the midline rostrally to the cerebellum, was observed. Histologically, highly cellular clusters of small-to-medium undifferentiated cells were intermingled with paucicellular areas with fibrillary eosinophilic (neuropil-like) appearance. Numerous multilayered (ependymoblastic) true rosettes were present. The mitotic activity was frequent (up to 15 mitoses/HPF), involving both undifferentiated cells and rosettes. By immunohistochemistry (IHC), tumor cells were diffusely positive for vimentin, variably for synaptophysin, S-100, and NSE, and focally for NeuN; they were negative for GFAP and CK AE1/AE3. The histological and IHC aspects were consistent with an Embryonal Tumor with Abundant Neuropil and True Rosettes (ETANTR). Embryonal neoplasms of the central nervous system (CNS) are characterized by primitive undifferentiated cells, able to develop toward neuronal, glial, ependymal, and mesenchymal lines. Although extremely rare, juvenile embryonal tumors should be considered in the differentials of CNS disorders in young cats.
Substance Use Among Residents of Homeless Shelters During the COVID-19 Pandemic: Findings From France
Authors: Scarlett Honor , Melchior Maria , Davisse-Paturet Camille , Aarbaoui Tarik El. , Longchamps Cécile , Figueiredo Natasha , Ducarroz Simon
Published: August 2022

Objectives: To record the prevalence and risk factors of substance use amongst homeless persons during the COVID-19 pandemic.

Methods: The ECHO study consisted in two independent cross-sectional waves of data collection in the regions of Paris, Lyon, and Strasbourg during the Spring of 2020 (n = 530) and 2021 (n = 319). Factors associated with substance use were explored using generalised logistic regression models.

Results: The most prevalent substance used was tobacco (38%–43%), followed by alcohol (26%–34%). The use of both substances positively associated with each other, although risk factors varied depending on the substance. The only factors consistently associated with alcohol and tobacco use were being male, exposure to theft/assault and participants’ region of origin. Whilst the rate of tobacco use was relatively stable between Spring 2020 and 2021, alcohol use was more common in 2021.

Conclusion: These findings highlight a high prevalence of substance use amongst homeless persons. People experiencing homelessness face specific challenges in the context of the pandemic, alongside greater vulnerability to illness and low healthcare access, therefore the need to improve prevention and support services for substance abuse within this population is vital.
A Multi-Center Study on the Negative Psychological Impact and Associated Factors in Chinese Healthcare Workers 1 Year After the COVID-19 Initial Outbreak
Authors: Gonzalez Mendez Maria Jose , Ma Li , Alvarado Ruben , Ramirez Jorge , Xu Kun-Peng , Xu Hui-Fang , Zhang Shao-Kai , Bangura Mohamed S. , Yang Ying , Yu Yan-Qin , Zhang Xi , Wang Wenjun , Gu Xiaofen , Li Li , Salah Didier Sama , Qiao Youlin
Published: August 2022

Objectives: The study aimed at analyzing the prevalence of five psychological outcomes (depression, anxiety, stress, post-traumatic stress disorder (PTSD), and suicidal ideation) among Chinese healthcare workers (HCWs), and measured the total possible negative psychological impact 1 year after the COVID-19 initial outbreak.

Methods: A cross-sectional nationwide multi-center study was performed between November 2020 and March 2021 in China. A self-report questionnaire was applied, and three psychological scales were used. Binary logistic regression was performed to analyze the risk factors associated with each psychological outcome.

Results: The findings demonstrated that the COVID-19 pandemic had a negative psychological impact on HCWs, which was still evident 1 year after the initial outbreak. Nurses showed higher depression and anxiety than other HCWs. Female gender, passive coping, long working hours, having a chronic disease, and experiencing violence, among other factors, were all risk factors for psychological impairment.

Conclusion: Developing and promoting programs to improve mental health among HCWs, and identifying those who might need psychological support is still relevant 1 year after the initial outbreak.
The Impact of Formality, Integration, and Commitment on the Performance of Latino-Owned Small Businesses
Authors: Starbird S. Andrew , Martin Jill M. , Posadas Yacanex , Gutiérrez Alma
Published: August 2022

Supporting the growth of minority-owned small- and medium-sized enterprises (SMEs) is an important goal in many countries. In the US, Latinos start businesses faster than any other demographic group, yet Latino wealth and income have not kept pace with the broader society. In this study, we explore the impact of different business and owner characteristics on the performance of Latino-owned small businesses. Using a survey of over 200 Latino small business owners in Silicon Valley, we apply ordered logistic regression to measure the effect of several variables on firm revenue. Our findings suggest that in addition to the gender and education of the owner, the most important factors impacting business performance are the owner’s commitment to the business, formal registration of the business, and the degree to which the business is integrated into the broader business community. This research adds to our understanding of the factors that significantly impact the economic success of Latino-owned small businesses. The results will be helpful to organizations that support the development and sustainability of Latino entrepreneurship.
Employee Compensation and Benefits Pre and Post COVID-19
Authors: Shtembari Eriona , Kufo Andromahi , Haxhinasto Dea
Published: August 2022

The Compensation and Benefits Package is considered the main employee’s motivator. As such, it plays a crucial role in determining successful recruiting, engagement, and retention strategies. Failing to offer the right package, will be translated into additional costs. This study aimed to identify what type of compensation and benefits Albanian employees receive and their relationship with job satisfaction, before and after COVID-19. We surveyed 127 employees and conducted 10 in-depth interviews, in different institutions in Albania. Descriptive and inferential statistics were used to analyze the data. The findings showed that most received benefits are medical insurance and bonuses, and employees are less than considerably satisfied with their package. The majority stated that it had great importance in their job satisfaction, and most would leave their current job for another one that offers more benefits. Findings related to the COVID-19 impact showed that employees’ lifestyle has changed and flexible working hours are the top benefit, followed by more paid time-off options. The conclusions of the study indicate that Compensations and Benefits packages should be redesigned to meet the needs of working in the “new normal”, for current and future employees, paying close attention to their preferences.
Effects of Dietary Supplementation with Glutamine on the Immunity and Intestinal Barrier Gene Expression in Broiler Chickens Infected with Salmonella Enteritidis
Authors: Wu Qiujue , Wang Cong , Liao Jiahui , Hu Naizhi , Cheng Binyao , Ma Yan , Wang Yuqin
Published: August 2022

The effects of glutamine (Gln) on immunity and intestinal barrier gene expression levels in broilers challenged with Salmonella Enteritidis were evaluated. A total of 400 1-day-old broilers were randomly assigned to four groups, 10 repetition treatments per group with 10 broiler chickens for a 21-day feeding trial. The groups were the normal control group (CON, no infected group, fed with a basal diet); the S. Enteritidis-infected control group (SCC, infected with 2.0 × 104 CFU/mL of S. Enteritidis, fed a basal diet); and the Gln 1 and 2 groups, who were challenged with S. Enteritidis and fed a basal diet plus Gln at 0.5% and 1.0%, respectively. The results show that S. Enteritidis had adverse effects on the average daily feed intake, average daily gain, and the feed conversion ratio of infected broilers compared with those of CON broilers on d 7 (p < 0.05); decreased serum immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) concentrations, and intestinal mucosa Bcl-2 mRNA expression levels (p < 0.05); increased the Lysozyme (LZM, only serum), NO, inducible NO synthase (iNOS) (except at 4 d), and total nitric oxide synthase (TNOS) (except at 4 d) activities in serum and the intestinal mucosa; and increased intestinal mucosa polymeric immunoglobulin receptor (pIgR) (except at 21 d), Avian beta-defensin 5 (AvBD5), AvBD14, Bax, and Bak mRNA expression levels during the experimental period (p < 0.05). Supplementation with Gln improved growth performance; increased serum IgA, IgG, and IgM concentrations and intestinal mucosa Bcl-2 mRNA expression levels (p < 0.05); decreased the LZM (only serum), NO, iNOS (except at 4 d), and TNOS (except at 4 d) activities in serum and the intestinal mucosa; and decreased intestinal mucosa pIgR (except at 21 d), AvBD5, AvBD14, Bax, and Bak mRNA expression levels during the experimental period (p < 0.05). These results suggest that Gln might lessen the inflammatory reaction of the small intestine and enlarge the small bowel mucosa immune and barrier function in broiler chickens challenged with S. Enteritidis.
Salivary Gland Adaptation to Dietary Inclusion of Hydrolysable Tannins in Boars
Authors: Mavri Maša , Čandek-Potokar Marjeta , Fazarinc Gregor , Škrlep Martin , Rutland Catrin S. , Potočnik Božidar , Batorek-Lukač Nina , Kubale Valentina
Published: August 2022

The ingestion of hydrolysable tannins as a potential nutrient to reduce boar odor in entire males results in the significant enlargement of parotid glands (parotidomegaly). The objective of this study was to characterize the effects of different levels of hydrolysable tannins in the diet of fattening boars (n = 24) on salivary gland morphology and proline-rich protein (PRP) expression at the histological level. Four treatment groups of pigs (n = 6 per group) were fed either a control (T0) or experimental diet, where the T0 diet was supplemented with 1% (T1), 2% (T2), or 3% (T3) of the hydrolysable tannin-rich extract Farmatan®. After slaughter, the parotid and mandibular glands of the experimental pigs were harvested and dissected for staining using Goldner’s Trichrome method, and immunohistochemical studies with antibodies against PRPs. Morphometric analysis was performed on microtome sections of both salivary glands, to measure the acinar area, the lobular area, the area of the secretory ductal cells, and the sizes of glandular cells and their nuclei. Histological assessment revealed that significant parotidomegaly was only present in the T3 group, based on the presence of larger glandular lobules, acinar areas, and their higher nucleus to cytoplasm ratio. The immunohistochemical method, supported by color intensity measurements, indicated significant increases in basic PRPs (PRB2) in the T3 and acidic PRPs (PRH1/2) in the T1 groups. Tannin supplementation did not affect the histo-morphological properties of the mandibular gland. This study confirms that pigs can adapt to a tannin-rich diet by making structural changes in their parotid salivary gland, indicating its higher functional activity.
The Consistent Variations of Precipitable Water and Surface Water Vapor Pressure at Interannual and Long-Term Scales: An Examination Using Reanalysis
Authors: Hao Jiawei , Lu Er
Published: August 2022

Water vapor (WV) is a vital basis of water and energy cycles and varies with space and time. When researching the variations of moisture in the atmosphere, it is intuitive to think about the total WV of the atmosphere column, precipitable water (PW). It is an element that needs high-altitude observations. A surface quantity, surface WV pressure (SVP), has a close relationship to PW because of the internal physical linkage between them. The stability of their linkage at climatic scales is verified using monthly mean data from 1979 to 2021, while studies before mainly focused on daily and annual cycles in local areas. The consistency of their variations is checked with three reanalysis datasets from three angles, the interannual variations, the long-term trends, and the empirical orthogonal function (EOF) modes. Results show that the interannual correlation of SVP and PW can reach a level that is quite high and are significant in most areas, and the weak correlation mainly exists over low-latitude oceans. The long-term trends, as well as the first EOF modes of these two quantities, also show that their variations are consistent, with spatial correlation coefficients between the long-term trends of two variables that are generally over 0.6, but specific differences appearing in some regions including the Tropical Indian Ocean and Middle Africa. With the correspondence of PW and SVP, the variations of total column WV can be indicated by surface elements. The correspondence is also meaningful for the analysis of the co-variation in total column vapor and temperature. For example, we could research the relations between SVP and air temperature, and they can reflect the co-variance of total column vapor and near-surface air temperature, which can avoid analyzing the relation between column-integrated moisture content and surface air temperature directly.
Immune Response to Biofilm Growing Pulmonary Pseudomonas aeruginosa Infection
Authors: Thomsen Kim , Høiby Niels , Jensen Peter Østrup , Ciofu Oana , Moser Claus
Published: August 2022

Biofilm infections are tolerant to the host responses and recalcitrance to antibiotic drugs and disinfectants. The induced host-specific innate and adaptive immune responses by established biofilms are significantly implicated and contributes to the course of the infections. Essentially, the host response may be the single one factor impacting the outcome most, especially in cases where the biofilm is caused by low virulent opportunistic bacterial species. Due to the chronicity of biofilm infections, activation of the adaptive immune response mechanisms is frequently experienced, and instead of clearing the infection, the adaptive response adds to the pathogenesis. To a high degree, this has been reported for chronic Pseudomonas aeruginosa lung infections, where both a pronounced antibody response and a skewed Th1/Th2 balance has been related to a poorer outcome. In addition, detection of an adaptive immune response can be used as a significant indicator of a chronic P. aeruginosa lung infection and is included in the clinical definitions as such. Those issues are presented in the present review, along with a characterization of the airway structure in relation to immune responses towards P. aeruginosa pulmonary infections.
Nanosensor Applications in Plant Science
Authors: Shaw Daniel S. , Honeychurch Kevin C.
Published: August 2022

Plant science is a major research topic addressing some of the most important global challenges we face today, including energy and food security. Plant science has a role in the production of staple foods and materials, as well as roles in genetics research, environmental management, and the synthesis of high-value compounds such as pharmaceuticals or raw materials for energy production. Nanosensors—selective transducers with a characteristic dimension that is nanometre in scale—have emerged as important tools for monitoring biological processes such as plant signalling pathways and metabolism in ways that are non-destructive, minimally invasive, and capable of real-time analysis. A variety of nanosensors have been used to study different biological processes; for example, optical nanosensors based on Förster resonance energy transfer (FRET) have been used to study protein interactions, cell contents, and biophysical parameters, and electrochemical nanosensors have been used to detect redox reactions in plants. Nanosensor applications in plants include nutrient determination, disease assessment, and the detection of proteins, hormones, and other biological substances. The combination of nanosensor technology and plant sciences has the potential to be a powerful alliance and could support the successful delivery of the 2030 Sustainable Development Goals. However, a lack of knowledge regarding the health effects of nanomaterials and the high costs of some of the raw materials required has lessened their commercial impact.
Experimental Investigation of a New Design of Insulation Gypsum Plaster Blocks
Authors: Binti Katman Herda Yati , Khai Wong Jee , Benjeddou Omrane , Mashaan Nuha
Published: August 2022

Green building materials are an alternative to ordinary materialsoffering multiple environmental benefits. This study consists of an experimental investigation of a new design of gypsum plaster blocks. First, a mix design of gypsum plaster and water mixture was prepared. The optimal mix composition was determined according to the mechanical and physical properties, such as the water absorption, the temperature of hydration, the density, and the compressive strength of different gypsum plaster and water mixtures made by varying the water dosage. The second part of this investigation aims to study a new design of green blocks prepared from the optimal water and gypsum plaster mixture. The new blocks are perforated to lighten them and to reduce their thermal conductivity in order to make them moreinsulate. Experimental tests were conducted on the block prototype, such as the measurement of dimensional tolerances, compressive strength, density, flatness, water absorption, residual moisture, surface hardness, and thermal conductivity. Experimental test results show that the new blocks have very low density, and their compressive strength is sufficient for wall construction. In addition, the manufacturing process of the new blocks is very easy and very fast. Finally, the obtained physical and mechanical properties of the new gypsum plaster blocks give it the opportunity to be used for interior walls for building constructions.
Cost-Effectiveness of an Organized Lung Cancer Screening Program for Asbestos-Exposed Subjects
Authors: Gendarme Sébastien , Pairon Jean-Claude , Andujar Pascal , Laurent François , Brochard Patrick , Delva Fleur , Clin Bénédicte , Gislard Antoine , Paris Christophe , Thaon Isabelle , Goussault Helene , Canoui-Poitrine Florence , Chouaïd Christos
Published: August 2022

Background: The National Lung Screening Trial (NLST) and NELSON study opened the debate on the relevance of lung cancer (LC) screening in subjects exposed to occupational respiratory carcinogens. This analysis reported the incremental cost-effectiveness ratios (ICER) of an organized LC screening program for an asbestos-exposed population. Methods: Using Markov modelization, individuals with asbestos exposure were either monitored without intervention or annual low-dose thoracic computed-tomography (LDTCT) scan LC screening. LC incidence came from a prospective observational cohort of subjects with occupational asbestos exposure. The intervention parameters were those of the NLST study. Utilities and LC-management costs came from published reports. A sensitivity analysis evaluated different screening strategies. Results: The respective quality-adjusted life year (QALY) gain, supplementary costs and ICER [95% confidence interval] were: 0.040 [0.010–0.065] QALY, 6900 [3700–11,800] € and 170,000 [75,000–645,000] €/QALY for all asbestos-exposed subjects; and 0.144 [0.071–0.216] QALY, 13,000 [5700–26,800] € and 90,000 [35,000–276,000] €/QALY for smokers with high exposure. When screening was based on biennial LDTCT scans, the ICER was 45,000 [95% CI: 15,000–116,000] €/QALY. Conclusions: Compared to the usual ICER thresholds, biennial LDTCT scan LC screening for smokers with high occupational exposure to asbestos is acceptable and preferable to annual scans.
Surgical Principles of Primary Retroperitoneal Sarcoma in the Era of Personalized Treatment: A Review of the Frontline Extended Surgery
Authors: Munoz Paula , Bretcha-Boix Pedro , Artigas Vicente , Asencio José Manuel
Published: August 2022

Surgery is the key treatment in retroperitoneal sarcoma (RPS), as completeness of resection is the most important prognostic factor related to treatment. Compartmental surgery/frontline extended approach is based on soft-tissue sarcoma surgical principles, and involves resecting adjacent viscera to achieve a wide negative margin. This extended approach is associated with improved local control and survival. This surgery must be tailored to tumor histology, tumor localization, and patient performance status. We herein present a review of compartmental surgery principles, covering the oncological and technical basis, and describing the tailored approach to each tumor subtype and localization in the retroperitoneum.
Functional Drug Screening of Small Molecule Inhibitors of Epigenetic Modifiers in Refractory AML Patients
Authors: Dennison Jessica L. , Al-Ali Hassan , Volmar Claude-Henry , Brothers Shaun , Watts Justin , Wahlestedt Claes , Lohse Ines
Published: August 2022

The use of inhibitors of epigenetic modifiers in the treatment of acute myeloid leukemia (AML) has become increasingly appealing due to the highly epigenetic nature of the disease. We evaluated a library of 164 epigenetic compounds in a cohort of 9 heterogeneous AML patients using an ex vivo drug screen. AML blasts were isolated from bone marrow biopsies according to established protocols and treatment response to the epigenetic library was evaluated. We find that 11 histone deacetylase (HDAC) inhibitors, which act upon mechanisms of cell cycle arrest and apoptotic pathways through inhibition of zinc-dependent classes of HDACs, showed efficacy in all patient-derived samples. Other compounds, including bromodomain and extraterminal domain (BET) protein inhibitors, showed efficacy in most samples. Specifically, HDAC inhibitors are already clinically available and can be repurposed for use in AML. Results in this cohort of AML patient-derived samples reveal several epigenetic compounds with high anti-blast activity in all samples, despite the molecular diversity of the disease. These results further enforce the notion that AML is a predominantly epigenetic disease and that similar epigenetic mechanisms may underlie disease development and progression in all patients, despite differences in genetic mutations.
Clinical Application of Pectoralis Nerve Block II for Flap Dissection-Related Pain Control after Robot-Assisted Transaxillary Thyroidectomy: A Preliminary Retrospective Cohort Study
Authors: Chae Min Suk , Park Youngkyung , Shim Jung-Woo , Hong Sang Hyun , Park Joonseon , Kang Il Ku , Bae Ja Seong , Kim Jeong Soo , Kim Kwangsoon
Published: August 2022

Few studies have examined the clinical utility of ultrasonography-guided pectoralis nerve block II (PECS II) during wide flap dissection of a robot-assisted transaxillary thyroidectomy (RATT). We assessed the ability of PECS II to reduce postoperative pain. We retrospectively reviewed 62 patients who underwent elective RATT from December 2021 to April 2022 at Seoul St. Mary’s Hospital (Seoul, Korea). The patients were divided into a block group (n = 28, 50.9%) and no-block group (n = 27, 49.1%). Pain was measured using a visual analog scale (VAS) at 4, 10, 20, 25, 35, and 45 h after surgery, and the requirements for rescue painkillers in the post-anesthesia care unit and ward were recorded. The VAS scores did not differ significantly between the two groups at 4 h postoperatively. The block group had significantly lower VAS scores at 10 and 25 h (p = 0.017 and p = 0.034, respectively). The block group required fewer painkillers in the post-anesthesia care unit than the no-block group, although the difference was not statistically significant in the ward. PECS II may serve as a new pain relief modality and valuable addition to the current multimodal analgesic strategy for patients undergoing RATT.
Immunization Combined with Ferroptosis Related Genes to Construct a New Prognostic Model for Head and Neck Squamous Cell Carcinoma
Authors: Yang Linhui , Chen Zhiwei , Liu Yunliang , Wang Xiaoyan , Li Jing , Ye Qing
Published: August 2022

Ferroptosis is a new type of programmed cell death that plays a pivotal role in a variety of tumors. Moreover, immunity is closely related to ferroptosis. However, immune-ferroptosis-related mRNAs (IFRMs) are still not fully understood in the regulation of head and neck squamous cell carcinoma (HNSC). The purpose of this paper was to investigate the IFRMs prediction of HNSC and its possible molecular biological role. RNA-Seq and related clinical data were mined from the TCGA database, ImmPort database, GeneCards database, FerrDb database, and previous data. In R software, the “DESeq2” package was used to analyze the differential expression of IFRMs. We used univariate Cox analysis to judge the prognosis of the IFRMs. Using the least absolute shrinkage and selection operator (LASSO) and Cox regression, a prediction model for 12 IFRMs was established. In this study, the Kaplan–Meier survival curve and receiver operating characteristic (ROC) curve analysis were used to evaluate the prediction results. Moreover, factors such as immune landscape, somatic mutations, and drug susceptibility are also discussed. We successfully constructed the signature of 12-IFRMs. The two risk groups were classified according to the risk score obtained by this signature. Compared with conventional clinicopathological features, the characteristic-based risk score was more predictive of survival in patients with HNSC. Furthermore, the expression of CD8+T cells and macrophage M0 differed significantly between the two groups. Moreover, the expression of TNFSF9 and CD44 in high-risk groups was significantly increased compared with the low-risk groups. Then, we found a higher proportion of high-risk mutations than in the low-risk group. Next, the high-risk group was more sensitive to chemotherapy drugs such as bosutinib, docetaxel, erlotinib, gefitinib, imatinib, lapatinib, and sorafenib. Finally, an in-depth analysis of the association and potential value of the 12 genes was performed. In summary, the 12-IFRM signatures established in this paper had good application prospects and could be effectively used to predict the clinical outcome and treatment response of head and neck squamous cell carcinoma.
Ring Opening Polymerization of Lactides and Lactones by Multimetallic Titanium Complexes Derived from the Acids Ph2C(X)CO2H (X = OH, NH2)
Authors: Zhang Xin , Prior Timothy J. , Chen Kai , Santoro Orlando , Redshaw Carl
Published: August 2022

The reactions of the titanium alkoxide [Ti(OR)4] (R = Me, nPr, iPr, tBu) with the acids 2,2′-Ph2C(X)(CO2H), where X = OH and NH2, i.e., benzilic acid (2,2′-diphenylglycolic acid, L1H2), and 2,2′-diphenylglycine (L2H3), have been investigated. The variation of the reaction stoichiometry allows for the isolation of mono-, bi-, tri or tetra-metallic products, the structures of which have been determined by X-ray crystallography. The ability of the resulting complexes to act as catalysts for the ring opening polymerization (ROP) of ε-caprolactone (ε-CL) and r-lactide (r-LA) has been investigated. In the case of ε-CL, all catalysts except that derived from [Ti(OnPr)4] and L2H3, i.e., 7, exhibited an induction period of between 60 and 285 min, with 7 exhibiting the best performance (>99% conversion within 6 min). The PCL products are moderate- to high-molecular weight polymers. For r-LA, systems 1, 3, 4 and 7 afforded conversions of ca. 90% or more, with 4 exhibiting the fastest kinetics. The molecular weights for the PLA are somewhat higher than those of the PCL, with both cyclic and linear PLA products (end groups of OR/OH) identified. Comparative studies versus the [Ti(OR)4] starting materials were conducted, and although high conversions were achieved, the control was poor.
Microwave-Assisted Photocatalytic Degradation of Organic Pollutants via CNTs/TiO2
Authors: Ren Yuqing , Chen Yao , Li Qinyu , Li Hexing , Bian Zhenfeng
Published: August 2022

Introducing microwave fields into photocatalytic technology is a promising strategy to suppress the recombination of photogenerated charge carriers. Here, a series of microwave-absorbing photocatalysts, xCNTs/TiO2, were prepared by combining titanium dioxide (TiO2) with carbon nanotubes (CNTs) using a typical alcoholic thermal method to study the promotion of microwave-generated thermal and athermal effects on the photocatalytic oxidation process. As good carriers that are capable of absorbing microwaves and conducting electrons, CNTs can form hot spots and defects under the action of the thermal effect from microwaves to capture electrons generated on the surface of TiO2 and enhance the separation efficiency of photogenerated electrons (e−) and holes (h+). Excluding the influence of the reaction temperature, the athermal effect of the microwave field had a polarizing effect on the catalyst, which improved the light absorption rate of the catalyst. Moreover, microwave radiation also promoted the activation of oxygen molecules and hydroxyl groups on the catalyst surface to generate more reactive oxygen radicals. According to the mechanism analysis, the microwave effect significantly improved the photocatalytic advanced oxidation process, which lays a solid theoretical foundation for practical application.
Beyond SMARCB1 Loss: Recent Insights into the Pathobiology of Epithelioid Sarcoma
Authors: Del Savio Elisa , Maestro Roberta
Published: August 2022

Epithelioid sarcoma (ES) is a very rare and aggressive mesenchymal tumor of unclear origin and uncertain lineage characterized by a prevalent epithelioid morphology. The only recurrent genetic alteration reported in ES as yet is the functional inactivation of SMARCB1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1), a key component of the SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling complexes. How SMARCB1 deficiency dictates the clinicopathological characteristics of ES and what other molecular defects concur to its malignant progression is still poorly understood. This review summarizes the recent findings about ES pathobiology, including defects in chromatin remodeling and other signaling pathways and their role as therapeutic vulnerabilities.
IRF8: Mechanism of Action and Health Implications
Authors: Moorman Hannah R. , Reategui Yazmin , Poschel Dakota B. , Liu Kebin
Published: August 2022

Interferon regulatory factor 8 (IRF8) is a transcription factor of the IRF protein family. IRF8 was originally identified as an essentialfactor for myeloid cell lineage commitment and differentiation. Deletion of Irf8 leads to massive accumulation of CD11b+Gr1+ immature myeloid cells (IMCs), particularly the CD11b+Ly6Chi/+Ly6G− polymorphonuclear myeloid-derived suppressor cell-like cells (PMN-MDSCs). Under pathological conditions such as cancer, Irf8 is silenced by its promoter DNA hypermethylation, resulting in accumulation of PMN-MDSCs and CD11b+ Ly6G+Ly6Clo monocytic MDSCs (M-MDSCs) in mice. IRF8 is often silenced in MDSCs in human cancer patients. MDSCs are heterogeneous populations of immune suppressive cells that suppress T and NK cell activity to promote tumor immune evasion and produce growth factors to exert direct tumor-promoting activity. Emerging experimental data reveals that IRF8 is also expressed in non-hematopoietic cells. Epithelial cell-expressed IRF8 regulates apoptosis and represses Osteopontin (OPN). Human tumor cells may use the IRF8 promoter DNA methylation as a mechanism to repress IRF8 expression to advance cancer through acquiring apoptosis resistance and OPN up-regulation. Elevated OPN engages CD44 to suppress T cell activation and promote tumor cell stemness to advance cancer. IRF8 thus is a transcription factor that regulates both the immune and non-immune components in human health and diseases.
PD-L1 Over-Expression Varies in Different Subtypes of Lung Cancer: Will This Affect Future Therapies?
Authors: Ullah Asad , Pulliam Steven , Karki Nabin Raj , Khan Jaffar , Jogezai Sana , Sultan Sandresh , Muhammad Lal , Khan Marjan , Jamil Nimra , Waheed Abdul , Belakhlef Sami , Ghleilib Intisar , Vail Eric , Heneidi Saleh , Karim Nagla Abdel
Published: August 2022

Programmed death-ligand (PD-L) 1 and 2 are ligands of programmed cell death 1 (PD-1) receptor. They are members of the B7/CD28 ligand-receptor family and the most investigated inhibitory immune checkpoints at present. PD-L1 is the main effector in PD-1-reliant immunosuppression, as the PD-1/PD-L pathway is a key regulator for T-cell activation. Activation of T-cells warrants the upregulation of PD-1 and production of cytokines which also upregulate PD-L1 expression, creating a positive feedback mechanism that has an important role in the prevention of tissue destruction and development of autoimmunity. In the context of inadequate immune response, the prolonged antigen stimulation leads to chronic PD-1 upregulation and T-cell exhaustion. In lung cancer patients, PD-L1 expression levels have been of special interest since patients with non-small cell lung cancer (NSCLC) demonstrate higher levels of expression and tend to respond more favorably to the evolving PD-1 and PD-L1 inhibitors. The Food and Drug Administration (FDA) has approved the PD-1 inhibitor, pembrolizumab, alone as front-line single-agent therapy instead of chemotherapy in patients with NSCLC and PD-L1 ≥1% expression and chemoimmunotherapy regimens are available for lower stage disease. The National Comprehensive Cancer Network (NCCN) guidelines also delineate treatment by low and high expression of PD-L1 in NSCLC. Thus, studying PD-L1 overexpression levels in the different histological subtypes of lung cancer can affect our approach to treating these patients. There is an evolving role of immunotherapy in the other sub-types of lung cancer, especially small cell lung cancer (SCLC). In addition, within the NSCLC category, squamous cell carcinomas and non-G12C KRAS mutant NSCLC have no specific targetable therapies to date. Therefore, assessment of the PD-L1 expression level among these subtypes of lung cancer is required, since lung cancer is one of the few malignances wherein PD-L1 expression levels is so crucial in determining the role of immunotherapy. In this study, we compared PD-L1 expression in lung cancer according to the histological subtype of the tumor.