bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanism of Bed Separation Water Inrush during the Mining of Extra-Thick Coal Seam under Super-Thick Sandstone Aquifer
Authors: Shi Xiuchang , Lyu Guangluo
Published: July 2023

The bed separation water inrush disasters in the Jurassic coalfield of Huanglong, China occur frequently, and they seriously threaten the safety of coal mining. This study systematically collected data from multiple instances of bed separation water inrush (BSWI) in the Cuimu coal mine and summarized the characteristics of BSWI. Through the analysis of hydrogeological conditions, hydrochemical characteristics, field detection of a water-conducting fractured zone (WCFZ), and groundwater level monitoring, the water inrush source, water-conducting channel, and the dynamic response of the aquifer water level were studied. The results showed that the water inrush source was mainly Cretaceous groundwater. The height of WCFZ in the extra-thick coal seam mining in the study area was 239.62 m, forming a water-conducting channel of water accumulation in bed separation. There was a strong correlation between water level changes in the Luohe Formation and BSWI. On this basis, we have proposed that four conditions must be met simultaneously for BSWI, and a hydrogeological-mining coupling conceptual model was established to reveal the evolution process and the mechanism of BSWI. The research results are crucial for the prevention of BSWI disasters and for ensuring the safety of coal mine production.
The Role of Contract Farming in Green Smart Agricultural Technology
Authors: Chen Junjin , Zhou Hong
Published: July 2023

Promoting green agricultural production is becoming increasingly important in order to address resource and environmental issues and meet consumers’ demand for safe agricultural products. Green agriculture mainly refers to the adoption of green, smart agriculture technologies in agricultural production. Farmers are the main body of agricultural production, so guiding farmers to adopt green, smart agriculture technologies is of great significance for safeguarding the ecological environment. However, due to the combined influence of internal and external factors, the current level of adoption of green, smart agriculture technologies by farmers is not high. Contract farming can have an impact on farmers’ production behavior through various channels, such as guiding and standardizing production, and may become an internal driving force for improving the adoption of green, smart agriculture technologies. How do farmers make contract farming selection decisions? How does the choice of contract farming affect farmers’ adoption of green, smart agriculture technologies? Further research on the above issues can provide useful references for promoting the development of green agriculture and ensuring the quality of agricultural products in China. Against this backdrop, this paper, using research data about rice farmers in the Jiangsu Province of China, adopted a gradual regression method for checking the mediating and moderating effects to discover the mechanism of how contract farming influences rice farmers’ choice of green, smart agriculture technologies. The research results showed that: (1) contract farming has an evidently positive influence on farmers’ choice of green, smart agriculture technologies; (2) the high ecological value standard plays a complete mediating role in the process of contract farming influencing farmers’ choice of green, smart agriculture technologies; and (3) the moderating effect of planting rice income exists and is significant. When the income from cultivation is high, the positive relationship between the high ecological value standard and farmers’ choice of green, smart agriculture technologies is strengthened. Income from rice cultivation does have a moderating effect. Therefore, the government can actively guide farmers to participate in contract farming by increasing its publicity and support of contract farming. The implementation of the high ecological value standard in contract farming should be refined. The government should complete the mechanism for selling quality agricultural products at good prices. Contract farming can make farmers and corporations involved have deep cooperation, increase the non-agricultural income of farmers, and further enhance the overall income of their households. Through these measures, the development of green agriculture can be achieved.
Feminine vs. Masculine: Expectations of Leadership Styles in Hotels during the COVID-19 Pandemic
Authors: Kachniewska Magdalena , Para Anna
Published: July 2023

Tourism and hospitality were among the first sectors to be severely affected by the COVID-19 pandemic and are still recovering from the crisis. In the first wave of the COVID-19 pandemic, a package of precautionary measures taken to contain the virus brought tourist activities to a complete standstill and changed tourists’ expectations and behavioral patterns. The COVID-19 pandemic presented the ultimate test for organizational leaders, who had had no chance to prepare (themselves) for the challenges that were to come. The leaders had to face uncertainty and make decisions that impacted the companies’ future success, understood as their continued existence. This study provides an up-to-date and innovative contribution to the areas of leadership and staff turnover in the hospitality industry from the perspective of transformational leadership theory. This article explores the leadership adopted by hotel managers in Poland during the first wave of the COVID-19 pandemic from March to December 2020 and offers useful insights into employees’ expectations towards leaders. A mixed methods approach was applied in the study, including focus groups and semi-structured interviews. The interviews were conducted in 35 hotels with 204 participants. The results point out that respondents preferred a feminine leadership style, at least in times of crisis, and thus statistically they most often cited “inclusivity, collaboration and empowerment”, “empathy and care”, and “transparency and communication” as desirable examples of leadership attributes. On the other hand, masculine attributes of crisis leadership, i.e., “resilience and courage” and “decisiveness and risk-taking” were indicated less frequently. An interesting result was a significant pool of responses indicating the search for various ways of adaptive leadership. This study provides a more nuanced, comprehensive assessment of the quality of staff attitudes in off-chain hotels in Poland, taking into account the impact of leadership style on staff turnover at the business unit level. The leadership lessons learned from the COVID-19 pandemic could possibly shed light on some key actionable attributes and skills that future leaders will need to develop to overcome (at least in the initial phases) any future crisis.
Planning Recreation around Water Bodies in Two Hard Coal Post-Mining Areas in Southern Poland
Authors: Kantor-Pietraga Iwona , Krzysztofik Robert , Solarski Maksymilian
Published: July 2023

Green and blue infrastructure is a unique aspect of sustainable development in post-industrial and post-mining cities. The article examines the issue of the characteristic post-mining lake district in one of Europe’s largest urban and hard coal mining regions—the Katowice Conurbation in Southern Poland. This article aims to clarify the conditions and problems of developing post-mining water bodies as an element of blue and green infrastructure, with a particular focus on the issue of developing recreational functions. The latter aspect was analyzed using extensive CAVI (Computer-Assisted Web Interview)/CATI (Computer-Assisted Telephone Interviewing) surveys conducted among residents, in-depth stakeholder interviews, and strategic and planning document analysis. This research confirmed that residents and local authorities treat post-mining reservoirs as necessary for creating green and blue infrastructure. Recreational functions were perceived as one of the elements of urban policy, in which the preservation of valuable ecosystems created around reservoirs has the same place. An essential element of the research findings is also the typology of post-mining reservoirs presented here, which allows for a better understanding of the development dilemmas of these areas in a highly urbanized and post-mining area.
Disentangling Decarbonisation Ambidexterity: An Analysis of European Companies
Authors: Zomer Thayla , Savaget Paulo
Published: July 2023

Corporate strategies to tackle climate change have become a topic of much debate. Yet, limited attention has been paid to understanding how companies exploit and explore decarbonisation opportunities. We posit that the risks imposed by climate change and the opportunities that emerge under such a context require companies to develop a decarbonisation ambidexterity capability to reconcile the exploration of more radical, long-term eco-innovation opportunities, while also being eco-efficient in their current activities. Based on the ambidexterity literature, we ask: How do companies explore and exploit to reduce their carbon footprint? Through an inductive analysis of the climate change reports of 410 European companies from different sectors, we identify seven core exploitative and explorative initiatives adopted by companies in response to climate change. We argue that, to reach the established targets, companies must adopt multiple initiatives focused on both exploring new knowledge and technologies and exploiting improvements in existing routines and processes, and we show how companies have combined these efforts.
Does the Digital Economy Improve the Innovation Efficiency of the Manufacturing Industry? Evidence in Provincial Data from China
Authors: Hui Ning , Yu Qian , Gu Yu
Published: July 2023

This paper selects panel data on 30 Chinese provinces from 2011 to 2020 and uses an econometric approach to empirically examine the impact of development in the digital economy on the innovation efficiency of China’s manufacturing industry. The results of this study show that during the period under investigation, the innovation efficiency of China’s manufacturing industry is in a stage of continuous improvement, and the development in the digital economy significantly promotes the level of manufacturing innovation efficiency, becoming an important driving force for the improvement in manufacturing innovation efficiency in the digital era. Moreover, the digital economy not only directly promotes manufacturing innovation efficiency but also has an indirect impact on such efficiency with industrial structure upgrading and technology spillover. With upgrading industrial structure and technology spillover, the digital economy has an enhanced effect on the innovation efficiency of the manufacturing industry. Using an analysis with the spatial Durbin model, it is found that the digital economy has a positive impact on the innovation efficiency of the manufacturing industry with spatial spillover characteristics.
Transforming Waste into Value: Eco-Friendly Synthesis of MOFs for Sustainable PFOA Remediation
Authors: Jery Atef El , Pecho Renzon Daniel Cosme , Tania Churampi Arellano Meryelem , Aldrdery Moutaz , Elkhaleefa Abubakr , Wang Chongqing , Sammen Saad Sh. , Tizkam Hussam H.
Published: July 2023

In response to the need for sustainable solutions to address perfluorooctanoic acid (PFOA) contamination, we have developed an eco-friendly approach for synthesizing two types of metal-organic frameworks (MOFs) using waste polyethylene terephthalate (PET) bottles via a one-pot microwave-assisted strategy. Our innovative method not only avoids the initial depolymerization of PET bottles but also promotes environmental conservation by recycling waste materials. The La-MOF and Zr-MOF materials exhibit remarkable surface areas of 76.90 and 293.50 m2/g, respectively, with La-MOF demonstrating greater thermal stability than Zr-MOF. The maximum experimental PFOA adsorption for La-MOF and Zr-MOF was obtained at 310 and 290 mg/g, respectively. Both MOFs follow the Langmuir isotherm closely, with the adsorption of PFOA following a pseudo-2nd-order kinetic model. In packed-bed column tests, breakthrough positions of 174 and 150 min were observed for La-MOF and Zr-MOF, respectively, with corresponding bed volumes of 452 mL and 522 mL based on the PFOA limit of 0.07 µg/L in drinking water. These findings indicate that these MOFs can be used in industrial packed-bed columns to remove PFOA from contaminated water sources in an efficient and cost-effective manner. Importantly, the sorption performance of the fabricated MOFs for PFOA remained stable, decreasing by less than 10% over seven cycles. This study underscores the potential of recycled PET bottles and the one-pot microwave-assisted synthesis of MOFs as an effective and environmentally friendly solution for PFOA remediation. This innovative approach has several managerial implications, such as the use of waste materials as a feedstock, which can reduce the cost of production and minimize environmental impact by promoting recycling and repurposing, enhancing the reputation of companies operating in the chemical industry, and improving their sustainability metrics. By integrating sustainability principles and waste recycling, our approach offers promising avenues for addressing PFOA contamination while promoting resource efficiency and environmental conservation.
Green Development Policies for China’s Manufacturing Industry: Characteristics, Evolution, and Challenges
Authors: Jin Weibo , Xu Lijie , Wu Shiping , Xu Yao , Han Shiwen
Published: July 2023

Since the start of the industrial revolution, the manufacturing industry has been essential for economic growth but has also contributed to environmental pollution problems. The United Nations declared the 2030 Sustainable Development Goals (SDG) agenda to make sure that the well-being of the global environment is taken care of alongside the expansion of the world economies. As the leading manufacturing country worldwide, studying the evolution of China’s green development policies in manufacturing has significant implications for pollution management in manufacturing in other countries. This research analyzes China’s legal and policy documents on green development in the manufacturing industry based on planning objectives and actual effects with qualitative content analysis. It divides them into four periods: the exploring period (1949–1977), the formal establishment period (1978–2001), the improvement and strengthening period (2002–2011), and the comprehensive improvement period (2012 to present). Although the Chinese government has made progress in implementing green development policies, it still faces many challenges: (1) compatibility between economic development and environmental protection needs to be strengthened; (2) primarily command-and-control based policy structure needs to be reformed; (3) collaboration of multi-departmental management system needs to be enhanced. These challenges are the primary obstacles to China’s manufacturing industry achieving its environmental goals. The future policies for the green development of the manufacturing industry should focus on three aspects: (1) aligning environmental and manufacturing policies in setting strategic objectives and benchmarks; (2) concentrating on the systemic nature of policies and the interdependence of policy tools; (3) enhancing processes for policy creation, implementation, monitoring, and evaluation.
A Study on Technology Use for Sustainable Graduate Education Internationalization at Home: Chinese Teachers’ Experiences and Perspectives
Authors: Xu Qian , Boudouaia Azzeddine
Published: July 2023

Diverse innovative strategies for achieving sustainable internationalization have emerged as a consequence of the continuing development of education and technology. Several academic institutions in China have made the pursuit of international recognition a central policy objective in an effort to achieve global prominence in the coming decades. This study aimed to explore the use of technology in promoting the sustainable internationalization of graduate education in China using teachers’ experiences and perspectives. In this regard, an assessment was conducted of the teachers’ experiences and perspectives on the aspects involved in the use of technology-based education for sustainable internationalization as well as the effect of these aspects on the internationalization of graduate education. The sample consisted of 806 teachers from different universities in China. A questionnaire was used to collect the data. The data analysis was carried out using SPSS 22, JASP and SmartPLS 4. The results showed that Chinese teachers had satisfactory experiences regarding the use of technology in internationalizing graduate education. Furthermore, teachers were highly satisfied with using technology to support collaborative online international learning, research cooperation, intercultural competence development and virtual mobility. The results also revealed that teachers were less likely to be satisfied with using technology to support open exchange programs and the internationalization of the curriculum. In addition, the results demonstrated that collaborative online international learning, research cooperation and intercultural competence development had a direct effect on the internationalization of graduate education. Based on the findings, it was concluded that the situation regarding graduate education in China is acceptable, while at the same time, support and more effort from the government and teachers are required.
Teleworkability, Preferences for Telework, and Well-Being: A Systematic Review
Authors: Urien Begoña
Published: July 2023

Telework has grown exponentially due to COVID-19, and has revealed itself as a useful work condition with a largely positive impact on employees’ well-being. Since many variables are involved in determining the relationships between telework and well-being, this paper clarifies the role of teleworkability, employees’ preference for telework, and telework intensity; specifically, how the first two variables impact on well-being through telework intensity. A systematic review was carried out between 2012 and 2022 to analyze how these variables relate. Scarce literature connecting these subjects showed that teleworkability and the preference for telework influence the amount of time employees wish to telework. Teleworkability and preference for telework need to be studied from a multilevel perspective since country-, company-, and individual-level characteristics impact on them. The results also confirmed that telework intensity establishes direct relationships with well-being, and it is essential to predict it. Hybrid work emerged as a new concept which captures the best combination between on-site work and telework. Based on their employees’ preferences, companies can introduce “hybrid-work flexible programs” to maximize its positive effects on well-being as well as being able to re-design their jobs to better fit their employees’ levels of telework expectations.
Does the Construction of High-Speed Rail Change the Development of Regional Finance?
Authors: Li Dongmei , Jiang Renai , Lu Zheyuan , Sun Shanghong , Wang Longguo
Published: July 2023

This study aims to examine the relationship between the high-speed rail (HSR) and regional financial development, employing 270 prefecture-level cities in China. With the increase in traffic lines, the network effect of the HSR gradually amplifies, and its impact is felt on both the “quality” and “quantity” of financial development. Different from using a single indicator, this paper comprehensively measures urban financial development from the perspectives of financial broadening and deepening based on financial indicators from six dimensions. By constructing a two-way fixed-effect regression model of urban panels, this paper presents that the HSR affects financial broadening. Specifically, the HSR reduces the excessive expansion of financial scale, the degree of financial agglomeration, and the financial risks in cities. Meanwhile, the HSR also promotes financial deepening, meaning the enhancement of urban financial efficiency and financial depth, as well as changes in financial structure. An indirect mechanism analysis demonstrates that population migration caused by the HSR motivates the transformation of financial elements among cities, which has varying degrees of impact on urban financial development. Overall, local governments should seize the opportunity of densifying the HSR network to promote the sustainable development of urban finance, adjust the structure, improve efficiency, reduce risks and gradually support urban finance to become better and stronger.
Symmetric and Asymmetric Crisscross Augmented Ladder Multilevel Inverter with Fuzzy Logic Controller Optimization
Authors: Arumugam Parimala , Subbaraman Srinath , Chandrasekaran Kannan
Published: July 2023

This research article presents a modified novel crisscross augmented ladder (CCAL) structured multilevel inverter (MLI). MLIs can be operated in symmetric and asymmetric binary and ternary voltage ratios. The modified structure comprises a generalized unit (CCAL) and an extendable structure; this structure can be extended to generate more stair case waveform. The foremost benefit of this modified structure is to curb the conduction path of active switches. The utilized structure uses only four active conduction paths in all modes. However, an MLI has complexity, such as a higher number of switches and bulky controlling driver circuits which need superior controls. This article suggests a prominent solution for the above issues. The subtle CCAL is a governed multicarrier pulse width modulation scheme with the savvy fuzzy logic controller and, therefore, added benefits, such as lower switch stress, lower switching loss, and lower dv/dt stress. Hitherto, many topologies are emerging to curb the component count reduction structure; among them it is an attempt to curtail the active conduction path. The working capability of the presented system is substantiated with a simulation study carried out in MATLAB R2017a and viability hardware (Xilinx FPGA) proof of concept to validate the effectiveness.
Symmetry in Privacy-Based Healthcare: A Review of Skin Cancer Detection and Classification Using Federated Learning
Authors: Yaqoob Muhammad Mateen , Alsulami Musleh , Khan Muhammad Amir , Alsadie Deafallah , Saudagar Abdul Khader Jilani , AlKhathami Mohammed , Khattak Umar Farooq
Published: July 2023

Skin cancer represents one of the most lethal and prevalent types of cancer observed in the human population. When diagnosed in its early stages, melanoma, a form of skin cancer, can be effectively treated and cured. Machine learning algorithms play a crucial role in facilitating the timely detection of skin cancer and aiding in the accurate diagnosis and appropriate treatment of patients. However, the implementation of traditional machine learning approaches for skin disease diagnosis is impeded by privacy regulations, which necessitate centralized processing of patient data in cloud environments. To overcome the challenges associated with data privacy, federated learning emerges as a promising solution, enabling the development of privacy-aware healthcare systems for skin cancer diagnosis. This paper presents a comprehensive review that examines the obstacles faced by conventional machine learning algorithms and explores the integration of federated learning in the context of privacy-conscious skin cancer prediction healthcare systems. It provides discussion on the various datasets available for skin cancer prediction and provides a performance comparison of various machine learning and federated learning techniques for skin lesion prediction. The objective is to highlight the advantages offered by federated learning and its potential for addressing privacy concerns in the realm of skin cancer diagnosis.
Toxicity and Lethal Effect of Greenhouse Insecticides on Coccinella septempunctata (Coleoptera: Coccinellidae) as Biological Control Agent of Myzus persicae (Hemiptera: Aphididae)
Authors: Skouras Panagiotis J. , Karanastasi Eirini , Lycoskoufis Ioannis , Demopoulos Vasilis , Darras Anastasios I. , Tsafouros Athanasios , Tsalgatidou Polina C. , Margaritopoulos John T. , Stathas George J.
Published: July 2023

Deltamethrin and imidacloprid are commonly used insecticides for controlling sub-sucking insects in greenhouses. However, their application may cause sublethal effects on the aphid coccinellid predator Coccinella septempunctata (Coleoptera: Coccinellidae). Here, we study (i) the toxicity and the effect of two sublethal doses (LD10 and LD30) of deltamethrin and imidacloprid on C. septempunctata in a laboratory microcosm and (ii) the residual toxicity of the two insecticides in a greenhouse. The results showed that both insecticides reduced fecundity, longevity, the intrinsic rate of increase, the finite rate of increase and the net reproductive rate. However, the developmental time of the fourth instar larvae was prolonged by both insecticides at LD10 and LD30. Deltamethrin residues were toxic 21 DAT (days after treatment) to C. septempunctata fourth instar larvae. In contrast, imidacloprid began in the slightly harmful category (75%) 1 DAT and declined to the harmless category (18.33%) 21 DAT. These results indicate that deltamethrin and imidacloprid have potential risks to C. septempunctata. This study provides information to guide the development of integrated pest management (IPM) strategies in greenhouses.
CdTe-QDs Affect Reproductive Development of Plants through Oxidative Stress
Authors: Wang Jianhua , Gong Yan , Yan Xiaoyan , Han Rong , Chen Huize
Published: July 2023

With the continuous development of industry, an increasing number of nanomaterials are widely used. CdTe-QDs is a nanomaterial with good optical properties, but its release into the natural environment may pose a potential threat. The toxicity of nanoparticles in plants is beginning to be questioned, and the effect on phytotoxicity is unclear. In this study, we simulated air pollution and soil pollution (CdTe-QDs concentrations of 0, 0.2, 0.4, 0.8 mmol/L) by spraying and watering the seedlings, respectively. We determined the transport pathways of CdTe-QDs in Arabidopsis thaliana and their effects on plant reproductive growth. Spraying CdTe-QDs concentration >0.4 mmol/L significantly inhibited the formation of fruit and decreased the number of seeds. Observation with a laser confocal scanning microscope revealed that CdTe-QDs were mainly transported in plants through the vascular bundle, and spraying increased their accumulation in the anthers and ovaries. The expression level of genes associated with Cd stress was analyzed through RT-qPCR. CdTe-QDs significantly increased the expression levels of 10 oxidative stress-related genes and significantly decreased the expression levels of four cell-proliferation-related genes. Our results reveal for the first time the transport of CdTe-QDs in Arabidopsis flowers and demonstrate that QDs can cause abnormal pollen morphology, form defects of pollen vitality, and inhibit pollen tube growth in Arabidopsis through oxidative damage. These phenomena ultimately lead to the inability of Arabidopsis to complete the normal fertilization process and affect the reproductive growth of the plant.
Integrative Chemical–Biological Grouping of Complex High Production Volume Substances from Lower Olefin Manufacturing Streams
Authors: Cordova Alexandra C. , Klaren William D. , Ford Lucie C. , Grimm Fabian A. , Baker Erin S. , Zhou Yi-Hui , Wright Fred A. , Rusyn Ivan
Published: July 2023

Human cell-based test methods can be used to evaluate potential hazards of mixtures and products of petroleum refining (“unknown or variable composition, complex reaction products, or biological materials” substances, UVCBs). Analyses of bioactivity and detailed chemical characterization of petroleum UVCBs were used separately for grouping these substances; a combination of the approaches has not been undertaken. Therefore, we used a case example of representative high production volume categories of petroleum UVCBs, 25 lower olefin substances from low benzene naphtha and resin oils categories, to determine whether existing manufacturing-based category grouping can be supported. We collected two types of data: nontarget ion mobility spectrometry-mass spectrometry of both neat substances and their organic extracts and in vitro bioactivity of the organic extracts in five human cell types: umbilical vein endothelial cells and induced pluripotent stem cell-derived hepatocytes, endothelial cells, neurons, and cardiomyocytes. We found that while similarity in composition and bioactivity can be observed for some substances, existing categories are largely heterogeneous. Strong relationships between composition and bioactivity were observed, and individual constituents that determine these associations were identified. Overall, this study showed a promising approach that combines chemical composition and bioactivity data to better characterize the variability within manufacturing categories of petroleum UVCBs.
Contamination Status and Ecological Security Thresholds of Fluoride in Farmland around a Phosphorus Chemical Plant in a Karst Area of Southwestern China
Authors: Guo Ziyu , Wang Min , Dai Hengmei , Pan Sha
Published: July 2023

The phosphorus chemical plant (PCP) production process leads to the substantial discharge of fluoride into the surrounding environment. However, there is limited research data regarding the pollution levels and ecological safety thresholds of farmland fluoride around PCPs in karst areas. This study evaluates the local contamination status and health risks by determining the fluoride content in farmland and vegetables in vicinity of a PCP in a karst area of Southwestern China. Farmland ecological security threshold was derived based on the species sensitivity distribution (SSD) model. Results showed that the fluoride contents in the soil ranged from 529.36 to 1496.02 mg kg−1, with the median value of 823.93 mg kg−1, which was higher than the national background value in China (478 mg kg−1) by 172.37%. Fluoride contents in vegetables ranged from 0.01 to 25.21 mg kg−1, with the median value of 1.29 mg kg−1, which was higher than the limits of contaminants in food (1 mg kg−1) by 129.00%, and 53.85% of vegetable samples were contaminated. Leafy vegetables showed a higher tendency of fluoride enrichment compared to non-leafy vegetables. Despite there being no significant health risk to the residents via the intake of vegetables overall, there may be potential health risks from the intake of sweet potato leaves. Based on the SSD model, the ecological safety thresholds of fluoride in local farmland was classified into the suitable for planting category (≤174.13 mg kg−1), safe plant utilization category (174.13–4005.42 mg kg−1), and strict control of planting category (≥4005.42 mg kg−1). These findings will provide valuable insights to facilitate the safe cultivation of local agricultural products and optimize the utilization of soil resources.
High-Level Expression of Recombinant VHSV Glycoprotein Using Transformed C. vulgaris and Verification of Vaccine Efficacy
Authors: Kim Min-Jeong , Kim Seon-Young , Kim Ki-Hong , Yoo Sung-Sik , Lee Taek-Kyun , Choi Tae-Jin
Published: July 2023

The demand for aquaculture is increasing, but production is declining due to high feed costs and disease outbreaks. Viral hemorrhagic septicemia (VHS) is a viral disease that seriously affects seawater and freshwater fish in aquaculture, including the olive flounder (Paralichthys olivaceus), a major aquaculture fish in Korea. However, very few vaccines are currently available for viral hemorrhagic septicemia virus (VHSV). The nutrient-rich microalga Chlorella vulgaris has been used as a feed additive in aquaculture and as a host for the industrial production of recombinant VHSV glycoprotein as an oral vaccine. In this study, VHSV glycoprotein was cloned with a salt-inducible promoter, and high levels of expression up to 41.1 mg/g wet C. vulgaris, representing 27.4% of total extracted soluble protein, were achieved by growing the transformed C. vulgaris for 5 days in the presence of 250 mM NaCl. The production of a neutralizing antibody was detected in the serum of fish given feed containing 9% VHSV glycoprotein-expressing C. vulgaris. Furthermore, relative survival rates of 100% and 81.9% were achieved following challenges of these fish with VHSV at 106 and 107 pfu/fish, respectively, indicating that C. vulgaris could be used as a platform for the production of recombinant proteins for use as oral vaccines in the control of viral diseases in aquaculture.
Effects of Anemoside B4 on Plasma Metabolites in Cows with Clinical Mastitis
Authors: Shen Liuhong , Shen Yu , Zhang Yue , Cao Suizhong , Yu Shumin , Zong Xiaolan , Su Zhetong
Published: July 2023

Anemoside B4 has a good curative effect on cows with CM; however, its impact on their metabolic profiles is unclear. Based on similar somatic cell counts and clinical symptoms, nine healthy dairy cows and nine cows with CM were selected, respectively. Blood samples were collected from cows with mastitis on the day of diagnosis. Cows with mastitis were injected with anemoside B4 (0.05 mL/kg, once daily) for three consecutive days, and healthy cows were injected with the same volume of normal saline. Subsequently, blood samples were collected. The plasma metabolic profiles were analyzed using untargeted mass spectrometry, and the concentrations of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) in serum were evaluated via ELISA. The cows with CM showed increased concentrations of IL-1β, IL-6, and TNF-α (p < 0.05). After treatment with anemoside B4, the concentrations of IL-1β, IL-6, and TNF-α were significantly decreased (p < 0.01). Untargeted metabolomics analysis showed that choline, glycocholic acid, PC (18:0/18:1), 20-HETE, PGF3α, and oleic acid were upregulated in cows with CM. After treatment with anemoside B4, the concentrations of PC (16:0/16:0), PC (18:0/18:1), linoleic acid, eicosapentaenoic acid, phosphorylcholine, and glycerophosphocholine were downregulated, while the LysoPC (14:0), LysoPC (18:0), LysoPC (18:1), and cis-9-palmitoleic acid were upregulated. This study indicated that anemoside B4 alleviated the inflammatory response in cows with CM mainly by regulating lipid metabolism.
Development and Application of nanoPCR Method for Detection of Feline Panleukopenia Virus
Authors: Xue Haowen , Liang Yang , Gao Xu , Song Yanhao , Zhu Kunru , Yang Meng , Hao Jingrui , Ma Haoyuan , Yu Kai
Published: July 2023

Feline panleukopenia (FP) is a severe viral illness caused by the feline panleukopenia virus (FPV), putting sectors like companion cat breeding and endangered feline conservation at risk. The virus has a high morbidity and fatality rate and is found all over the world. We created a novel FPV assay using nanoPCR technology and assessed the method’s specificity and sensitivity. The approach amplified a 345 bp nucleic acid fragment with a minimum detection limit of 7.97 × 102 copies/μL, which is about 100 times greater than traditional PCR. We collected anal swabs from 83 cats suspected of FPV infection for practical application, and the FPV-positive rate determined by the nanoPCR approach was 77.1%. In conclusion, the approach is more sensitive than conventional PCR and more convenient and cost-effective than qPCR methodology and may be utilized for the clinical detection of FPV.
Runoff Prediction Based on Dynamic Spatiotemporal Graph Neural Network
Authors: Yang Shuai , Zhang Yueqin , Zhang Zehua
Published: July 2023

Runoff prediction plays an important role in the construction of intelligent hydraulic engineering. Most of the existing deep learning runoff prediction models use recurrent neural networks for single-step prediction of a single time series, which mainly model the temporal features and ignore the river convergence process within a watershed. In order to improve the accuracy of runoff prediction, a dynamic spatiotemporal graph neural network model (DSTGNN) is proposed considering the interaction of hydrological stations. The sequences are first input to the spatiotemporal block to extract spatiotemporal features. The temporal features are captured by the long short-term memory network (LSTM) with the self-attention mechanism. Then, the upstream and downstream distance matrices are constructed based on the river network topology in the basin, the dynamic matrix is constructed based on the runoff sequence, and the spatial dependence is captured by combining the above two matrices through the diffusion process. After that, the residual sequences are input to the next layer by the decoupling block, and, finally, the prediction results are output after multi-layer stacking. Experiments are conducted on the historical runoff dataset in the Upper Delaware River Basin, and the MAE, MSE, MAPE, and NSE were the best compared with the baseline model for forecasting periods of 3 h, 6 h, and 9 h. The experimental results show that DSTGNN can better capture the spatiotemporal characteristics and has higher prediction accuracy.
Impact of Different Design Rainfall Pattern Peak Factors on Urban Flooding
Authors: Chen Jian , Li Yaowei , Zhang Shanju
Published: July 2023

In order to investigate the influence of different design rainfall peaks on urban flooding characteristics based on the MIKE hydrodynamic model, the inundation process scenarios were extrapolated for different recurrence periods and three single- and double-peak rainfall types in Zhoukou city as an example, and the equivalent values of total inundation and inundation area were compared and analysed. The results show that bimodal rainfall has a higher risk of inundation than unimodal rainfall for the same rainfall ephemeris and return period. For unimodal rainfall, when the return period is less than 20 years, the more advanced the rainfall peak, the more severe the design rainfall inundation. When the return period is greater than 20 years, the further back the rainfall peak, the more severe the inundation of the design rainfall. The difference between the risk of inundation due to single- and double-peaked rainfall decreases as the return period increases.
Modeling BTEX Multiphase Partitioning with Soil Vapor Extraction under Groundwater Table Fluctuation Using the TMVOC Model
Authors: Yang Yang , Zheng Jingwei , Li Juan , Huan Huan , Zhao Xiaobing , Lv Ningqing , Ma Yan , Zhang Hao
Published: July 2023

The effects of groundwater table fluctuation (GTF) on the remediation of a petrochemically polluted riverside using soil vapor extraction (SVE) were investigated. The migration and transformation of benzene, toluene, ethylbenzene, and o-xylene (BTEX) in cases of natural attenuation, SVE without GTF, and SVE with GTF were simulated using the TMVOC model. The results showed that the optimized extraction well pressure and influencing radius of the target site were 0.90 atm and 8 m, respectively. The removal rates of BTEX in cases of natural attenuation, SVE without GTF, and SVE with GTF were 11.49%, 85.16%, and 97.33%, respectively. The removal rate of BTEX was maximized in the case of SVE with a GTF amplitude of 0.5 m to 1 m. The removal rates of benzene (99.99%), toluene (99.74%), ethylbenzene (96.37%), and o-xylene (94.72%) were maximized in the case of SVE with GTF. For the cases of SVE without GTF and SVE with GTF, mass losses of BTEX in gaseous (0.05 kg, 0.05 kg, respectively) and aqueous phases (5.46 kg, 5.87 kg, respectively) were consistent. However, the mass loss of BTEX in the non-aqueous phase liquid (NAPL) phase in the case of SVE with GTF (155.13 kg) exceeded that in the case of SVE without GTF (135.41 kg). This is because GTF positively affected both the solubility and volatility of BTEX in the NAPL phase. With the groundwater table decreasing, flows of gas and gaseous pollutants increased by 25% along the vertical section. At this stage, the removal rates of volatile organic compounds can be further improved by increasing the flow of the extraction well.
Guarantees of protection of individual labor rights of employees in the labor legislation of Russia and Kazakhstan
Authors:   Akmola Regional Court, Republic of Kazakhstan, Ospanova B.A.
Published: July 2023

The article is devoted to the protection of individual labor rights of employees in the presence of a status «preponderance » of the employer. The article examines the relationship between the protection of individual labor rights of an employee and guarantees of such protection under the legislation of Russia and Kazakhstan, suggests ways to improve the list of ways to protect workers’ labor rights.
Comprehensive assessment of the performance results of organizations of the Agricultural Industrial Complex
Authors:   Russian Timiryazev State Agrarian University, Moscow, Russia, Khoruzhy L. I. , Tryastsina N. Yu. ,   Russian Timiryazev State Agrarian University, Moscow, Russia, Chernyshev A. S. ,   Russian Timiryazev State Agrarian University, Moscow, Russia, Durneva О. V. ,   Russian Timiryazev State Agrarian University, Moscow, Russia
Published: July 2023

The article is devoted to the issues of analysis, evaluation and accounting of factors influencing the occurrence of agro-industrial complex and the effectiveness of their activities. Based on a survey of systemic application, a methodology for a comprehensive assessment of the performance of economic entities is proposed. The results were obtained, which were used to assess the reserves of the efficiency of the economic activity of agribusiness organizations, develop a development strategy, justify business plans and management decisions at various levels.
Self-Diffusion Coefficient of Water in the Presence of Alkali Halide Ions
Authors: Baranyai András
Published: July 2023

The self-diffusion coefficient of water in aqueous solutions of alkali halides at different concentrations was studied using the polarizable BK3 model of water [1]. As ion potentials, initially the alkali halide force field fitted to this water potential by the solvation free energy, ion-water clusters as well as, for the purpose of crosschecking, to the ambient crystal energy and density was investigated. It was found that the strength of attraction between the central ion and the water molecules in the hydration shell is stronger than in reality, which manifests itself in the reduced mobility of water. For small ions, this discrepancy is small. By omitting the dipolar polarization of larger ions, the estimates could be improved. Similarly, increasing the size of the anions within reasonable limits also enhances the results but the structural breaking effect is insufficient. If the relative permittivity of water is smaller than that measured experimentally, typically ionic charges in non-polarizable water models can be decreased to recover the correct semi-macroscopic Coulomb energy. Since our polarizable water model reasonably estimates the relative permittivity, smaller ionic charges were used to quantify the differences in the strengths of ion-water attraction. Our estimates of self-diffusion in aqueous solutions by varying the concentration of ions are presented and discussed for LiCl, NaCl, NaBr, NaI, RbF and RbI.
Evaluación del Rendimiento de una red IPv6 utilizando IPSec en Modo Túnel
Authors: Ponce Loor Yumber Alejandro , Herrera Tapia Jorge Sergio
Published: July 2023

El espacio de direcciones IPv4 está a punto de llegar a su límite y podría colapsar, para corregir este evento muchos proponen que las redes existentes deberán realizar la migración definitiva a IPv6, tomando como referencia que este protocolo permitirá un mayor espacio de direcciones IP, brindando nuevas oportunidades y corrigiendo los problemas existentes en el protocolo de la versión anterior. Sin embargo, debido al aumento de la sobrecarga en IPv6 y su interacción con routers de borde y sistemas operativos que alojan este protocolo de comunicación, se pueden ocasionar evidentemente problemas de rendimiento en la red. El presente artículo se basa en una investigación cuantitativa utilizando un método que permite analizar el rendimiento de redes basadas en IPv6 con IPSec haciendo uso de la simulación con GNS3, con dicha herramienta compara resultados con tres escenarios típicos de implementación en relación a la topología original del presente trabajo, su evaluación determina la diferencia de rendimiento entre escenarios cuando se genera tráfico de una red a otra y se obtiene de entre ellos el un escenario con mejor estabilidad y rendimiento utilizando una metodología experimental, con lo que logramos constatar que su escenario homólogo con utilización de túneles en más óptimo en relación al propuesto en esta investigación, tomando en consideración que las métricas analizadas son ancho de banda, latencia y jitter.
Essential characteristics of threats and challenges to innovation and investment development in the system of regional economic security
Authors: Bank O.
Published: July 2023

In the article, the author, based on studying the problem of ensuring the economic security of the region, based on the consideration of innovative and investment aspects according to the proposed author's classification, substantiates the possibility of solving it at the level of individual subjects of the federation through their detailed study, systematization and assessment of the impact of their destructive consequences on the qualitative state of the economic systems as a whole.
Methodological approaches in the development of proposals for the formation of a tax expenditure management system
Authors: Mikhina E.
Published: July 2023

The article provides a systematic analysis of methodological approaches that allow developing proposals on the formation of a tax expenditure management system on a scientific basis. The author substantiates the need for a comprehensive study of the formation of a tax expenditure management system using complementary methods of different levels, formulates the principles, conditions and possibilities of their application, focuses on achieving the expected results. The main trends of tax expenditures in modern Russia, which determine the need to create a system for managing them, are considered.
Sustainability of budgets of the subjects of the Russian Federation: Problems of interpretation and approaches to its definition
Authors: Masterov A.
Published: July 2023

The article deals with the problems of determining budget sustainability, regarding the interpretation of which there is no consensus. The existing basic approaches to determining the budgetary sustainability of the subjects of the Russian Federation are disclosed.
PELATIHAN APLIKASI AKUNTASNI (USAHA JASA) UNTUK MENINGKATKAN KOMPETENSI SISWA JURUSAN IPS DI SMA NEGERI 11 KOTA JAMBI
Authors: Meisak Despita , Feranika Ayu , Rosario Maria , Masgo
Published: July 2023

Permasalahan yang sering terjadi akibat dari faktor akademik dibidang pendidikan akuntansi bagi siswa-siswi SMA khususnya jurusan IPS, yaitu hanya mempelajari dasar pembukuan akuntansi secara manual, hal ini membuat guru ekonomi khawatir dari hasil pembelajaran yang kurang optimal terhadap soft skill yang dimiliki jurusan IPS padahal identik keahliannya dibidang ekonomi. Pada kegiatan pengabdian kepada masyarakat ini peserta nya adalah siswa siswi SMAN 11 Kota Jambi jurusan IPS, dimana pihak sekolah belum menerapkan aplikasi akutansi pada mata pelajaran ekonomi yaitu ilmu akutansi, Oleh karena itu, pengabdian ini bertujuan memberikan solusi dengan memilih sistem informasi akuntansi dengan menggunakan aplikasi sederhana usaha jasa sehingga dapat meningkatkan kompetensi Siswa Jurusan IPS di SMAN 11 Kota Jambi dalam penyajian laporan keuangan Jasa. Metode pelaksanaan untuk kegiatan pengabdian ini dengan langkah-langkah berikut; (1) melakukan sosialisasi, pengenalan aplikasi akuntansi, (2) simulasi dan praktek bagaimana melakukan pencatatan setiap transaksi yang diberikan dengan aplikasi akutansi, (3) pendampingan penggunaan aplikasi akuntansi, (4) evaluasi