bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards an AI-driven soft toy for automatically detecting and classifying infant-toy interactions using optical force sensors
Authors: Udayagiri Rithwik , Yin Jessica , Cai Xinyao , Townsend William , Trivedi Varun , Shende Rohan , Sowande O. Francis , Prosser Laura A. , Pikul James H. , Johnson Michelle J.
Published: March 2024

Introduction: It is crucial to identify neurodevelopmental disorders in infants early on for timely intervention to improve their long-term outcomes. Combining natural play with quantitative measurements of developmental milestones can be an effective way to swiftly and efficiently detect infants who are at risk of neurodevelopmental delays. Clinical studies have established differences in toy interaction behaviors between full-term infants and pre-term infants who are at risk for cerebral palsy and other developmental disorders.

Methods: The proposed toy aims to improve the quantitative assessment of infant-toy interactions and fully automate the process of detecting those infants at risk of developing motor delays. This paper describes the design and development of a toy that uniquely utilizes a collection of soft lossy force sensors which are developed using optical fibers to gather play interaction data from infants laying supine in a gym. An example interaction database was created by having 15 adults complete a total of 2480 interactions with the toy consisting of 620 touches, 620 punches—“kick substitute,” 620 weak grasps and 620 strong grasps.

Results: The data is analyzed for patterns of interaction with the toy face using a machine learning model developed to classify the four interactions present in the database. Results indicate that the configuration of 6 soft force sensors on the face created unique activation patterns.

Discussion: The machine learning algorithm was able to identify the distinct action types from the data, suggesting the potential usability of the toy. Next steps involve sensorizing the entire toy and testing with infants.
Adequacy to immunosuppression management guidelines in kidney transplant recipients with severe COVID-19 pneumonia: a practice survey
Authors: Jacq Amélie , Auvray Christelle , Blot Mathieu , Bouhemad Belaïd , Casenaz Alice , Lamarthée Baptiste , Legendre Mathieu , Quenot Jean-Pierre , Zanetta Gilbert , Tinel Claire
Published: March 2024

Introduction

Coronavirus disease 2019 (COVID-19) poses an important risk of morbidity and of mortality, in patients after solid organ transplantation. Recommendations have been issued by various transplantation societies at the national and European level to manage the immunosuppressive (IS) regimen upon admission to intensive care unit (ICU).

Method

The aim of this study was to evaluate the adequacy of IS regimen minimization strategy in kidney transplant recipients hospitalized in an ICU for severe COVID-19, in relation to the issued recommendations.

Results

The immunosuppressive therapy was minimized in all patients, with respectively 63% and 59% of the patients meeting the local and european recommendations upon admission. During ICU stay, IS was further tapered leading to 85% (local) and 78% (european) adequacy, relative to the guidelines. The most frequent deviation was the lack of complete withdrawal of mycophenolic acid (22%). Nevertheless, the adequacy/inadequacy status was not associated to the ICU- or one-year-mortality.

Discussion

In this single-center cohort, the only variable associated with a reduction in mortality was vaccination, emphasizing that the key issue is immunization prior to infection, not restoration of immunity during ICU stay.
LoRa Communication Quality Optimization on Agriculture Based on the PHY Anti-Frame Loss Mechanism
Authors: Dai Qiufang , Chen Ziwei , Wu Guanfa , Li Zhen , Lv Shilei , Huang Weicheng
Published: March 2024

Agricultural environments are usually characterized by height differences and tree shading, which pose challenges for communication in smart agriculture. This study focuses on optimizing the packet loss rate and power consumption of LoRa’s practical communication quality. The research includes the investigation of the PHY anti-frame loss mechanism, encompassing PHY frame loss detection and the response mechanism between gateways and nodes. By implementing a closed loop for transmission and reception, the study enhances the communication network’s resistance to interference and security. Theoretical performance calculations for the SX1278 radio frequency chip were conducted under different parameters to determine the optimal energy efficiency, reducing unnecessary energy waste. An experimental assessment of the packet loss rate was conducted to validate the practical efficacy of the research findings. The results show that the LoRa communication with the anti-frame loss mechanism and the optimal energy ratio parameter exhibits an adequate performance. In the presence of strong and weak interferences, the reception rates are maximally improved by 37.8% and 53.4%, with effective distances of 250 m and 600 m, corresponding to enhancements of 100 m and 400 m, respectively. This research effectively reduces LoRa energy consumption, mitigates packet loss, and extends communication distances, providing insights for wireless transmission in agricultural contexts.
Environmental Performance of a Mixed Crop–Dairy Cattle Farm in Alexandria (Romania)
Authors: Ben Abdallah Saker , Gallego-Elvira Belén , Popa Dana Catalina , Maestre-Valero José Francisco , Imbernón-Mulero Alberto , Popa Razvan Alexandru , Bălănescu Mihaela
Published: March 2024

Agricultural specialization has increased considerably in Europe over the last decades, leading to the separation of crop and livestock production at both farm and regional levels. Such a transformation is often associated with higher environmental burdens due to excessive reliance on exogenous inputs and manure management issues. Reconnecting crop and livestock production via mixed farming systems (MFSs) could improve circularity and resilience, leading to reduced environmental impacts. The objective of this study was to evaluate the life cycle environmental performance of a commercial mixed crop–dairy cattle farm in Romania and to compare it against the corresponding specialized systems. The evaluation covered both dairy cattle production (milk and meat) and cash crops. Overall, the results show that the coupled system improves environmental performance by reducing the over-reliance on high-impact inputs like synthetic fertilizers and exogenous feed. The carbon footprint for the milk production of the studied system (1.17 kg CO2 eq.) per kg of fat- and protein-corrected milk (FPCM) was 10% lower than the mean value of common intensive milk production systems. The eutrophication impacts (2.52 × 10−4 kg P eq and 2.67 × 10−4 kg N eq./kg of FPCM) presented values of one order of magnitude less than their specialized counterparts. However, the impacts of the studied MFS, albeit lower than those for comparable specialized systems, still remain relatively high. In particular, methane emissions from enteric fermentation (0.54 kg CO2 eq./kg FPCM) were a major contributor to the carbon footprint. This highlighted the need to address the elevated emissions from enteric fermentation with better feed management, as well as improving and reinforcing the system’s self-sufficiency.
Mechanical Analysis of Semi-Rigid Base Asphalt Pavement under the Influence of Groundwater with the Spectral Element Method
Authors: Zhang Bei , Wang Di , Zhong Yanhui , Li Xiaolong , Cai Hongjian , Wang Tao
Published: March 2024

Over prolonged exposure to groundwater conditions, semi-rigid base asphalt pavements can undergo significant changes in their internal moisture field, resulting in substantial variations in the pavement’s stiffness and, consequently, affecting the overall load-bearing capacity and stability of the road structure. This paper employs FWD non-destructive testing equipment to assess its mechanical performance and conduct data analysis and conducts a mechanical response study of asphalt road surfaces, considering the influence of roadbed moisture levels. Using the dynamic analytical theory, the fundamental equations and stiffness matrices for a linear elastic half-space model were established, leading to the development of a computational model for the mechanical response of semi-rigid base asphalt pavements under FWD dynamic loading, with an examination of the surface deflection in relation to changes in groundwater levels. Numerical examples and engineering applications were employed to validate the proposed model. The research findings indicate: With the passage of time, surface deflection values initially increase and then decrease, exhibiting a sinusoidal variation pattern similar to that of the applied load. As the distance from the loading center increases, the moment of peak deflection continually lags behind. The average absolute relative error between the results obtained using the method proposed in this study and the traditional ABAQUS finite element method was only 0.70%. The correlation coefficient between the theoretically computed deflection curve and the measured deflection curve using the spectral element method was greater than 0.9, with an average absolute relative error of 4.92% between the theoretical peak deflection and the measured peak deflection. As the groundwater level rises, surface deflection noticeably increases, with an approximately 40% increase in deflection values at the loading center. These research findings can be utilized to analyze the dynamic deflection of semi-rigid base asphalt pavements under various groundwater conditions, providing significant practical value for assessing road structural performance and serviceability.
Optimizing GANs for Cryptography: The Role and Impact of Activation Functions in Neural Layers Assessing the Cryptographic Strength
Authors: Singh Purushottam , Dutta Sandip , Pranav Prashant
Published: March 2024

Generative Adversarial Networks (GANs) have surfaced as a transformative approach in the domain of cryptography, introducing a novel paradigm where two neural networks, the generator (akin to Alice) and the discriminator (akin to Bob), are pitted against each other in a cryptographic setting. A third network, representing Eve, attempts to decipher the encrypted information. The efficacy of this encryption–decryption process is deeply intertwined with the choice of activation functions employed within these networks. This study conducted a comparative analysis of four widely used activation functions within a standardized GAN framework. Our recent explorations underscore the superior performance achieved when utilizing the Rectified Linear Unit (ReLU) in the hidden layers combined with the Sigmoid activation function in the output layer. The non-linear nature introduced by the ReLU provides a sophisticated encryption pattern, rendering the deciphering process for Eve intricate. Simultaneously, the Sigmoid function in the output layer guarantees that the encrypted and decrypted messages are confined within a consistent range, facilitating a straightforward comparison with original messages. The amalgamation of these activation functions not only bolsters the encryption strength but also ensures the fidelity of the decrypted messages. These findings not only shed light on the optimal design considerations for GAN-based cryptographic systems but also underscore the potential of investigating hybrid activation functions for enhanced system optimization. In our exploration of cryptographic strength and training efficiency using various activation functions, we discovered that the “ReLU and Sigmoid” combination significantly outperforms the others, demonstrating superior security and a markedly efficient mean training time of 16.51 s per 2000 steps. This highlights the enduring effectiveness of established methodologies in cryptographic applications. This paper elucidates the implications of these choices, advocating for their adoption in GAN-based cryptographic models, given the superior results they yield in ensuring security and accuracy.
A Study on the Surrogate-Based Optimization of Flexible Wings Considering a Flutter Constraint
Authors: Lunghitano Alessandra , Afonso Frederico , Suleman Afzal
Published: March 2024

Accounting for aeroelastic phenomena, such as flutter, in the conceptual design phase is becoming more important as the trend toward increasing the wing aspect ratio forges ahead. However, this task is computationally expensive, especially when utilizing high-fidelity simulations and numerical optimization. Thus, the development of efficient computational strategies is necessary. With this goal in mind, this work proposes a surrogate-based optimization (SBO) methodology for wing design using a predefined machine learning model. For this purpose, a custom-made Python framework was built based on different open-source codes. The test subject was the classical Goland wing, parameterized to allow for SBO. The process consists of employing a Latin Hypercube Sampling plan and subsequently simulating the resulting wing on SHARPy to generate a dataset. A regression-based machine learning model is then used to build surrogate models for lift and drag coefficients, structural mass, and flutter speed. Finally, after validating the surrogate model, a multi-objective optimization problem aiming to maximize the lift-to-drag ratio and minimize the structural mass is solved through NSGA-II, considering a flutter constraint. This SBO methodology was successfully tested, reaching reductions of three orders of magnitude in the optimization computational time.
Physical and Finite Element Models for Determining the Capacity and Failure Mechanism of Helical Piles Placed in Weak Soil
Authors: Emirler Buse
Published: March 2024

The foundations of particular engineering structures, including marine and jetty structures, mooring systems for submerged platforms or those on the ocean surface, and transmission towers, are subjected to various external loads including compression, uplift, and lateral loads. In such cases, to improve the soil resistance below foundations, pile foundations such as helical piles, anchored piles, and batter piles are commonly preferred, depending on the in situ conditions. Helical piles, increasingly used as an alternative foundation to conventional piles, are placed in the soil body by rotating with torque. This paper deals with the contribution of a helical pile in improving loose sandy soil, and the main purpose is to study the effect of the helix-buried depth on the load-bearing capacity and failure mechanism. The investigated variables include the distance between helixes, the number of helixes, and the diameter of the upper helix. Physical model tests were conducted, and two- and three-dimensional numerical analyses were performed by using the finite element method with an advanced soil model to illustrate the failure mechanisms of helical piles. The aim was to reveal the efficiency of the finite element method in modelling helical piles placed in weak sandy soil. A simplified linear geometry for helixes was established in a two-dimensional finite element model whereas a real geometry for helixes, which was a more realistic approach, was created in a three-dimensional finite element model. The results show that the three-dimensional model indicates better agreement with the physical model compared to the two-dimensional model, and all investigated variables highly affect the load-bearing capacity of helical piles.
Zinc Oxide-Based Rotational–Linear Triboelectric Nanogenerator
Authors: Bardakas Achilleas , Segkos Apostolos , Tsamis Christos
Published: March 2024

In this study, we evaluate a prototype harvesting device that converts rotational motion to linear motion for harvesting rotational energy. Triboelectric materials are attached to the parts of the device that execute linear motion, resulting in a contact-separation mode of operation of triboelectric generators. As a triboelectric material, thin layers of ZnO nanoparticles deposited on Kapton films are evaluated. The design of the rotational–linear triboelectric nanogenerator (RL-TENG) exhibits several advantages since it does not suffer from the issues related to rotational tribogenerators such as wear and increased temperature during operation. Moreover, our approach can result in the modular design of energy-harvesting devices for a variety of applications. As a demonstrator, cups were attached to the rotating axis of the RL-TENG to harvest wind energy that is suitable for maritime applications.
Critical Ergonomics and (Dis)Comfort Factors While Performing Tasks with Hand Tools on a Ladder: A Pilot Study
Authors: Califano Rosaria , Auricchio Antonio , Carbone Mario , Dessì Lucio , Frasci Valentino , Landi Angelo , Naddeo Alessandro
Published: March 2024

Falls at height are among the major causes of fatal accidents at work, especially in the construction sector. Maintaining balance while performing a tool with both hands generates physical efforts and mental loading that needs to be managed for workers’ safety and health. The purpose of the study is to compare perceived (dis)comfort (overall and localized), the ergonomic risk, and the perceived effort through the acquired postures that subjects assumed during the execution of simple and common tasks both on a ladder and on the ground. 26 subjects were asked to perform four tasks in both conditions: top screwing, frontal screwing, object picking from the bottom forward, and object picking from the bottom sideways. Subjective and objective data were gathered for assessing subjective experience (discomfort and effort, perceived) and ergonomic risk. Results highlighted few ergonomic risk differences among “on ladder” and “on ground” tasks, while subjective data reveal significant differences in different scenarios. Furthermore, the knees and neck areas are the most critical ones.
Bridging the Vantage Point of Distance: Reynaldo Rivera and the Visual Legacies of Queer Spectacle across Time and Space
Authors: Vélez Estefanía
Published: March 2024

Gender impersonators and trans gender-nonconforming people have long been a source of fascination within the visual arts. Nevertheless, illustrators and photographers alike have perpetually instrumentalized the image of the queer subject as a visual shorthand for criminality, freakishness, and deception. Beginning with the broadside illustrations of José Guadalupe Posada, this article examines how visual representations of Latinx queerness and gender nonconformity shifted across the Americas and throughout the late nineteenth century into the late twentieth century. Ultimately, I contend that Reynaldo Rivera’s photography of late-twentieth-century ballroom culture provides a substantial departure from these speculatory conventions by visually legitimizing the lived authenticity of the queer Latinx people who populate his work.
Biocontrol Potential of Sodin 5, Type 1 Ribosome-Inactivating Protein from Salsola soda L. Seeds
Authors: Novak Babič Monika , Ragucci Sara , Leonardi Adrijana , Pavšič Miha , Landi Nicola , Križaj Igor , Gunde-Cimerman Nina , Sepčić Kristina , Di Maro Antimo
Published: March 2024

Sodin 5 is a type 1 ribosome-inactivating protein isolated from the seeds of Salsola soda L., an edible halophytic plant that is widespread in southern Europe, close to the coast. This plant, known as ‘agretti’, is under consideration as a new potential crop on saline soils. Considering a possible defence role of sodin 5 in the plant, we report here its antifungal activity against different halophilic and halotolerant fungi. Our results show that sodin 5 at a concentration of 40 µg/mL (1.4 µM) was able to inhibit the growth of the fungi Trimmatostromma salinum (35.3%), Candida parapsilosis (24.4%), Rhodotorula mucilaginosa (18.2%), Aspergillus flavus (12.2%), and Aureobasidium melanogenum (9.1%). The inhibition observed after 72 h was concentration-dependent. On the other hand, very slight growth inhibition was observed in the fungus Hortaea werneckii (4.2%), which commonly inhabits salterns. In addition, sodin 5 showed a cytotoxic effect on the Sf9 insect cell line, decreasing the survival of these cells to 63% at 1.0 µg/mL (34.5 nM). Structural analysis of sodin 5 revealed that its N-terminal amino acid residue is blocked. Using mass spectrometry, sodin 5 was identified as a homologous to type 1 polynucleotide:adenosine glycosylases, commonly known as ribosome-inactivating proteins from the Amaranthaceae family. Twenty-three percent of its primary structure was determined, including the catalytic site.
Co-Culture Models: Key Players in In Vitro Neurotoxicity, Neurodegeneration and BBB Modeling Studies
Authors: Monteiro Ana Rita , Barbosa Daniel José , Remião Fernando , Silva Renata
Published: March 2024

The biological barriers existing in the human body separate the blood circulation from the interstitial fluid in tissues. The blood–brain barrier (BBB) isolates the central nervous system from the bloodstream, presenting a dual role: the protection of the human brain against potentially toxic/harmful substances coming from the blood, while providing nutrients to the brain and removing metabolites. In terms of architectural features, the presence of junctional proteins (that restrict the paracellular transport) and the existence of efflux transporters at the BBB are the two major in vivo characteristics that increase the difficulty in creating an ideal in vitro model for drug permeability studies and neurotoxicity assessments. The purpose of this work is to provide an up-to-date literature review on the current in vitro models used for BBB studies, focusing on the characteristics, advantages, and disadvantages of both primary cultures and immortalized cell lines. An accurate analysis of the more recent and emerging techniques implemented to optimize the in vitro models is also provided, based on the need of recreating as closely as possible the BBB microenvironment. In fact, the acceptance that the BBB phenotype is much more than endothelial cells in a monolayer has led to the shift from single-cell to multicellular models. Thus, in vitro co-culture models have narrowed the gap between recreating as faithfully as possible the human BBB phenotype. This is relevant for permeability and neurotoxicity assays, and for studies related to neurodegenerative diseases. Several studies with these purposes will be also presented and discussed.
Immunological Similarities and Differences between Post-COVID-19 Lung Sequelae and Idiopathic Pulmonary Fibrosis
Authors: Gangi Sara , Bergantini Laura , Cameli Paolo , Paggi Irene , Spalletti Marco , Mezzasalma Fabrizio , Bargagli Elena , d’Alessandro Miriana
Published: March 2024

Introduction: Pulmonary fibrosis is an irreversible condition that may be caused by known (including viral triggers such as SARS-CoV-2) and unknown insults. The latter group includes idiopathic pulmonary fibrosis (IPF), which is a chronic, progressive fibrosing interstitial pneumonia of unknown cause. The longer the insult acts on lung tissue, the lower the probability of a complete resolution of the damage. An emerging clinical entity post-COVID-19 is pulmonary fibrosis (PCPF), which shares many pathological, clinical, and immunological features with IPF. The fibrotic response in both diseases—IPF and PCPF—is orchestrated in part by the immune system. An important role regarding the inhibitory or stimulatory effects on immune responses is exerted by the immune checkpoints (ICs). The aim of the present study was to analyse the similarities and differences between CD4+, CD8+, and NK cells in the peripheral blood of patients affected by fibrotic disease, IPF, and PCPF compared with sarcoidosis patients and healthy controls. The second aim was to evaluate the expression and co-expression of PD-1 and TIGIT on CD4, CD8, and NK cells from our patient cohort. Methods: One hundred and fifteen patients affected by IPF, PCPF, and sarcoidosis at the rare pulmonary disease centre of the University of Siena were enrolled. Forty-eight patients had an IPF diagnosis, 55 had PCPF, and 12 had sarcoidosis. Further, ten healthy controls were enrolled. PCPF patients were included between 6 and 9 months following hospital discharge for COVID-19. The peripheral blood samples were collected, and through flow cytometric analysis, we analysed the expression of CD4, CD8, NK cells, PD-1, and TIGIT. Results: The results show a greater depletion of CD4 and NK cells in IPF patients compared to other groups (p = 0.003), in contrast with CD8 cells (p < 001). Correlation analysis demonstrated an indirect correlation between CD4 and CD8 cells in IPF and sarcoidosis patients (p < 0.001 = −0.87 and p = 0.042; r = −0.6, respectively). Conversely, PCPF patients revealed a direct correlation between CD4 and CD8 cells (p < 0.001; r = 0.90) accentuating an immune response restoration. The expression of PD-1 and TIGIT was abundant on T and NK cell subsets of the two lung fibrotic groups, IPF and PCPF. Analogously, the co-expression of PD-1 and TIGIT on the surfaces of CD4 and CD8 were increased in such diseases. Conclusions: Our study shines a spotlight on the immune responses involved in the development of pulmonary fibrosis, idiopathic and secondary to SARS-CoV-2 infection. We observed a significant imbalance not only in CD4, CD8, and NK blood percentages in IPF and PCPF patients but also in their functional phenotypes evaluated through the expression of ICs.
Fatal Deterioration of a Respiratory Syncytial Virus Infection in an Infant with Abnormal Muscularization of Intra-Acinar Pulmonary Arteries: Autopsy and Histological Findings
Authors: Salfi Nunzio Cosimo Mario , Vergine Gianluca , Poloni Maurizio , Metalli Sara , Bigucci Barbara , Facondini Francesca , Pedrazzi Gianmatteo , Masciopinto Francesca , Bernabè Laura , Sambri Vittorio , Bonasoni Maria Paola
Published: March 2024

Respiratory syncytial virus (RSV) infection represents a global and noteworthy cause of hospitalization and death in infants of less than 1 year of age. The typical clinical manifestation is bronchiolitis, an inflammatory process of the small airways. The symptoms are usually a brief period of low-grade fever, cough, coryza, breathing difficulties, and reduced feeding. The progression of the disease is difficult to predict, even in previous healthy subjects. Symptoms may also be subtle and underestimated, thus leading to sudden unexpected infant death (SUID). In these cases, RSV infection is discovered at autopsy, either histologically or through real-time reverse transcription polymerase chain reaction (RT-PCR) performed on nasopharyngeal swabs. Herein, we describe a case of RSV infection in a 6-month-old infant with no risk factors, who rapidly deteriorated and unexpectedly died of respiratory insufficiency in a hospital setting. RT-PCR on nasopharyngeal swabs revealed RSV. The autopsy showed diffuse lymphogranulocytic bronchitis and bronchiolitis, and multiple foci of acute pneumonia. Abnormal muscularization of the intra-acinar pulmonary arteries was also observed, which likely contributed to worsening the lung impairment.
Comparison of Transient Elastography and Point Shear Wave Elastography for Analysis of Liver Stiffness: A Prospective Study
Authors: Losurdo Giuseppe , Ditonno Ilaria , Novielli Domenico , Celiberto Francesca , Iannone Andrea , Castellaneta Antonino , Dell’Aquila Paola , Ranaldo Nunzio , Rendina Maria , Barone Michele , Ierardi Enzo , Di Leo Alfredo
Published: March 2024

Liver stiffness measurement (LSM) by Fibroscan is the most used non-invasive method to assess liver fibrosis. Recently, point-shear wave elastography (pSWE) has been introduced as a simple alternative non-invasive test. Therefore, we aimed to compare the results of these two techniques. One hundred and eighty-four consecutive patients attending our outpatient ultrasound clinic were recruited. LSM was performed by both Fibroscan and pSWE. Statistical analysis was conducted by Spearman’s test for correlation and linear regression. Bland–Altman graphs and ROC curves were drawn with area under the curve (AUC). Overall, the correlation of LS between Fibroscan and pSWE was substantial (r = 0.68, p < 0.001). Linear regression showed a coefficient b= 0.94 ± 0.02. The Bland–Altman plot found a bias of −0.10, with only 11 values exceeding the 95% confidence interval. When only considering patients with a LSM of > 10 kPa (n = 31), we found an excellent r = 0.79 (0.60–0.90, p < 0.001). A cutoff of 12.15 kPa for pSWE had sensitivity = 74.2% and specificity = 99.3% to detect relevant fibrosis, with an AUC = 0.98. The highest correlation was observed for hepatitis C (r = 0.91) and alcoholic liver disease (ALD)(r = 0.99). In conclusion, pSWE shows LSM estimation in agreement with Fibroscan in most cases, and the best concordance was observed for hepatitis C and ALD, and for higher ranges of LS.
Exploring New Horizons for Wine Grapes: Modulating Functional Effects by Varying Harvest Timing and Solar Exposure
Authors: Rico Daniel , Schorn-García Daniel , Aceña Laura , García-Casas María Jesús , Busto Olga , Boqué Ricard , Mestres Montserrat , Martín-Diana Ana Belén
Published: March 2024

Grenache (GN) and Cabernet Sauvignon (CS) are two traditional red grape varieties widely cultivated in the Mediterranean area and both late-ripening cultivars, which makes them less sensitive to global warming conditions and more stable to harvest timing. Although different studies have evaluated the final antioxidant properties of grapes and pomaces, few studies have explored the effect of sun exposure and harvest on the nutritional and antioxidant properties of these products. This study investigates the control of sunlight and ripening as tools to tailor nutritional and antioxidant properties of grape juices (GJ) and their byproducts (pomace GP). The compositional analysis showed no significant (p ≥ 0.05) differences associated to either harvesting timing or exposure to sunlight for either of the two studied varieties. However, differences (p ≤ 0.05) were observed between varieties of protein and total dietary fibre (TDF). CS protein content ranged from 0.52 to 3.88 (g 100 g−1) in GJ and from 1.0 to 1.32 (g 100 g−1) in GP; meanwhile, GN had higher protein values in GJ (from 2.11 to 4.77 g 100 g−1) and GP (from 5.11 to 6.75 g 100 g−1). The opposite behaviour was observed in TDF; CS grape had higher values for juice (from 11.43 to 19.53 g 100 g−1) and pomace (from 42.20 to 65.80 g 100 g−1) than GN (from 11.43 to 17.22 g 100 g−1 in juice and from 25.90 to 54.0 g 100 g−1 in pomace). The total phenolic content (TP) in GP was 100 times higher than in the juices and showed a much less pronounced evolution compared to the GJ during the harvesting time. GN TP values ranged from 5835 to 8772 mg GAE 100 g−1; meanwhile, CS values ranged from 7637 to 9040 mg GAE 100 g−1. A significant (p ≤ 0.05) correlation between the TP total antioxidant capacity (TAC) results was observed, regardless of variety, harvesting time, and sunlight exposure. These findings show how the control of different factors can contribute to obtain modified grape-derived products from conventional varieties beyond the wine market.
Lithium-Associated Hypothyroidism in a Specialized Lithium Clinic at a Tertiary Care Hospital in Riyadh, Saudi Arabia: A Retrospective Study
Authors: Almadani Ahmad H. , Algazlan Maha S. , Alfraiji Abdulaziz F. , Almalki Nawaf Y.
Published: March 2024

Hypothyroidism is a common side effect of lithium use and is associated with a slower response to treatment and poorer long-term remission in patients with bipolar disorder. No studies have examined the prevalence of lithium-associated hypothyroidism or its associated factors in Saudi Arabia. We aimed to estimate the prevalence of lithium-associated hypothyroidism among psychiatric patients in a specialized lithium clinic at a tertiary care hospital in Saudi Arabia and to examine the possible risk factors for its development. This retrospective observational study included 117 participants. The prevalence of secondary hypothyroidism was 15%. The median duration between the start of lithium therapy and the first abnormal thyroid test result was 341 days. The multiple logistic regression showed that none of the studied variables, namely, sex, current age, age of lithium initiation, continuity on lithium, lithium level (≤0.5 mmol/L or >0.5 mmol/L), and prescription of as-needed medications, was significantly associated with secondary hypothyroidism. Our study also shed light on the possible clinical significance of baseline TSH levels in developing hypothyroidism secondary to lithium. Further multicenter studies with larger sample sizes are warranted to examine the generalizability of these results.
Positive and Negative Effects of Administering a Magnetic Field to Patients with Rheumatoid Arthritis (RA)
Authors: Zwolińska Jolanta , Kasprzak Marta , Kielar Aleksandra , Prokop Michał
Published: March 2024

Background: Magnetotherapy applied to patients with rheumatoid arthritis (RA) produces anti-inflammatory, analgesic and antioedema effects. Observations suggest that the beneficial and adverse effects of magnetotherapy are related to the parameters of the magnetic field applied. This study aimed to assess the positive and negative effects of magnetotherapy, taking into account the type of the field. Methods: This study involved 39 patients with RA, who were randomly assigned to two groups: SMF—static magnetic field (n = 18) and PEMF—low-frequency pulsed electromagnetic field (n = 21). The examinations carried out before and after the therapy included a general assessment of the functional status, assessment of pain severity, measurement of the duration and severity of morning stiffness, computer-aided measurement of the range of motion of the hand joints and measurement of the hand volume using water displacement method. The patients received kinesiotherapy and magnetotherapy, as determined by the randomisation. Results: The findings show improved functional status by 0.26 points on average (p = 0.0166) measured with the Health Assessment Questionnaire (HAQ-20), reduced pain by 2.2 points on average (p = 0.0000) on the Visual Analogue Scale (VAS), decreased duration of morning stiffness by 23.2 min on average (p = 0.0010) and reduced severity of morning stiffness by 15.2 points on average (p = 0.0010). The assessment of the dominant hand showed improved range of motion by 1.9 mm on average (p = 0.0036) and reduced volume by 0.9 mm3 on average (p = 0.0230). A significantly reduced duration and severity of morning stiffness was observed in the SMF group. Statistically significant changes in the HAQ-20 scores, range of motion and the volume of the dominant hand were identified in the PEMF group. Conclusions: Magnetic fields improved the functional status and reduced pain, morning stiffness and swelling in the hand. A static magnetic field may be more effective in reducing morning stiffness, whereas a pulsed magnetic field may, to a greater extent, improve function and reduce swelling in the rheumatoid hand. The effects of magnetotherapy reported so far require further observation.
Implementation of the Care Bundle for the Management of Chronic Obstructive Pulmonary Disease with/without Heart Failure
Authors: Bianco Andrea , Canepa Marco , Catapano Giosuè Angelo , Marvisi Maurizio , Oliva Fabrizio , Passantino Andrea , Sarzani Riccardo , Tarsia Paolo , Versace Antonio Giovanni
Published: March 2024

Chronic obstructive pulmonary disease (COPD) is often part of a more complex cardiopulmonary disease, especially in older patients. The differential diagnosis of the acute exacerbation of COPD and/or heart failure (HF) in emergency settings is challenging due to their frequent coexistence and symptom overlap. Both conditions have a detrimental impact on each other’s prognosis, leading to increased mortality rates. The timely diagnosis and treatment of COPD and coexisting factors like left ventricular overload or HF in inpatient and outpatient care can improve prognosis, quality of life, and long-term outcomes, helping to avoid exacerbations and hospitalization, which increase future exacerbation risk. This work aims to address existing gaps, providing management recommendations for COPD with/without HF, particularly when both conditions coexist. During virtual meetings, a panel of experts (the authors) discussed and reached a consensus on the differential and paired diagnosis of COPD and HF, providing suggestions for risk stratification, accurate diagnosis, and appropriate therapy for inpatients and outpatients. They emphasize that when COPD and HF are concomitant, both conditions should receive adequate treatment and that recommended HF treatments are not contraindicated in COPD and have favorable effects. Accurate diagnosis and therapy is crucial for effective treatment, reducing hospital readmissions and associated costs. The management considerations discussed in this study can potentially be extended to address other cardiopulmonary challenges frequently encountered by COPD patients.
Fall Treatment with Fumagillin Contributes to an Overwinter Shift in Vairimorpha Species Prevalence in Honey Bee Colonies in Western Canada
Authors: Biganski Sarah , Obshta Oleksii , Kozii Ivanna , Koziy Roman , Zabrodski Michael W. , Jose Midhun S. , Thebeau Jenna M. , Silva Marina C. B. , Raza Muhammad F. , Masood Fatima , Wood Sarah C. , Simko Elemir
Published: March 2024

(1) Background: Microsporidiosis (nosemosis) is an intestinal disorder of adult honey bees caused by the microsporidian pathogens Vairimorpha apis and Vairimorpha ceranae. In Canada, fumagillin is an approved antibiotic used to treat this disease. However, the recommended dosage is based on efficacy studies for V. apis, the native pathogen in European honey bees. Since the detection of V. ceranae in Apis mellifera, V. ceranae became more prevalent in managed European honey bees and seems to have replaced V. apis due to yet unknown reasons. (2) Methods: This colony study investigated the efficacy of fumagillin administered in the fall to colonies infected with both V. apis and V. ceranae and its effects on the Vairimorpha species’ prevalence overwinter. Spore loads in control and fumagillin-treated colonies were analysed by microscopy; Vairimorpha species prevalence was determined molecularly and infection and treatment effects on colony productivity were assessed. (3) Results: Fall fumagillin treatment was associated with a temporary reduction in spore load, but there was no difference in spore loads between treated and control colonies the following spring. Interestingly, fumagillin-treated colonies had a significantly greater prevalence of V. ceranae relative to V. apis the following spring, suggesting fumagillin is less effective in controlling V. ceranae.
In Vitro Study of Interleukin-6 when Used at Low Dose and Ultra-Low Dose in Micro-Immunotherapy
Authors: Jacques Camille , Marchand Flora , Chatelais Mathias , Brulefert Adrien , Riffault Mathieu , Floris Ilaria
Published: March 2024

As one of the major cytokines implicated in the orchestration of immune responses, interleukin 6 (IL-6) can either act as a pro- or an anti-inflammatory factor, depending on the micro-environment. In micro-immunotherapy (MI) medicines, IL-6 is employed at low doses (LD) and ultra-low doses (ULD), expressed in centesimal Hahnemannian (CH), and used alone or in combination with other immune regulators to modulate patients’ immune responses. The present study focused on assessing the in vitro immune-modulatory effects of two IL-6-containing MI products: (i) the unitary IL-6 (4 CH) and (ii) the complex MI-medicine (MIM) 2LALERG®, which includes IL-6 (17 CH) in association with other actives in its formulation. Our results showed that IL-6 (4 CH) activated granulocytes under basal conditions, and natural killer cells in the presence of an anti-CD3 signal, as assessed by their CD69 expression. In addition, IL-6 (4 CH) balanced the macrophages’ differentiation toward a M2a profile. On the other hand, the tested 2LALERG® capsule inhibited the histamine degranulation of rats’ peritoneal mast cells and reduced the release of IL-6 itself in inflamed human macrophages. Altogether, these data provide novel pieces of evidence on the double-edged potential of the LD and ULD of IL-6 in immune responses modulation, when employed in MI.
Machine Learning Application of Generalized Gaussian Radial Basis Function and Its Reproducing Kernel Theory
Authors: Singh Himanshu
Published: March 2024

Gaussian Radial Basis Function Kernels are the most-often-employed kernel function in artificial intelligence for providing the optimal results in contrast to their respective counterparts. However, our understanding surrounding the utilization of the Generalized Gaussian Radial Basis Function across different machine learning algorithms, such as kernel regression, support vector machines, and pattern recognition via neural networks is incomplete. The results delivered by the Generalized Gaussian Radial Basis Function Kernel in the previously mentioned applications remarkably outperforms those of the Gaussian Radial Basis Function Kernel, the Sigmoid function, and the ReLU function in terms of accuracy and misclassification. This article provides a concrete illustration of the utilization of the Generalized Gaussian Radial Basis Function Kernel as mentioned earlier. We also provide an explicit description of the reproducing kernel Hilbert space by embedding the Generalized Gaussian Radial Basis Function as an L2−measure, which is utilized in implementing the analysis support vector machine. Finally, we provide the conclusion that we draw from the empirical experiments considered in the manuscript along with the possible future directions in terms of spectral decomposition of the Generalized Gaussian Radial Basis Function.
Hydrogel- and Nanocomposite-Based Drug-Delivery Strategies in the Treatment of Vaginal Infections
Authors: AlAnsari Renad , Hasan Bushra , Deen G. Roshan , Torsten Uwe
Published: March 2024

The reproductive health of women is governed by an optimal balance in the host–microbiota interaction. Depletion of the beneficial vaginal microflora caused by depletion of Lactobacillus species and increased proliferation of pathogens results in gynaecological infections. Among women of reproductive age, vaginal infections are increasingly prevalent. Attaining therapeutic efficacy using conventional formulations remains a challenge as vaginal fluids quickly remove or dilute the therapeutic formulations. Hydrogels have been widely exploited for targeted delivery of therapeutics directly into the vaginal mucus. With a careful choice of polymers (natural, synthetic, or semisynthetic), hydrogels with specific properties, such as stimuli responsiveness, antimicrobial, and muco-adhesiveness, can be tailored for higher therapeutic efficacy. In this review, the advances in hydrogel strategies for the treatment of vaginal infections are presented with emphasis on the types and properties that play a significant role in vaginal drug delivery systems.
Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production
Authors: Fontes Afonso , Francisco Ricardo , Ferreira Frederico Castelo , Faria Nuno Torres , Marques Susana , Reis Alberto , Moura Patrícia , Lukasik Rafal , Santos José , Lopes da Silva Teresa
Published: March 2024

Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further cultivated in a bench bioreactor, using the same culture medium, under a batch regime. Flow cytometry was used to monitor the T. oleaginosus culture using the dual staining technique (SYBR Green and PI) for cell membrane integrity detection. Values of 42.28% (w/w) and 0.06 g/Lh lipid content and lipid productivity, respectively, were recorded for T. oleaginosus cultivated in the bench bioreactor operated under a batch regime. During the cultivation, most of the yeast cells maintained their integrity. T. oleaginosus has the potential to be used as an oil microbial source for a wide range of industrial applications. In addition, it is robust in adverse conditions such as lignocellulosic hydrolysate exposure and oxygen-limiting conditions. Flow cytometry is a powerful and useful tool for monitoring yeast cultivations on lignocellulosic hydrolysates for cell count, size, granularity, and membrane integrity detection.
Inequalities in Academic Work during COVID-19: The Intersection of Gender, Class, and Individuals’ Life-Course Stage
Authors: Carreri Anna , Naldini Manuela , Tuselli Alessia
Published: March 2024

Research studies on academic work and the COVID-19 crisis have clearly shown that the pandemic crisis contributed to exacerbating pre-existing gender gaps. Although the research has been extensive in this regard, it has focused more on the widening of the “motherhood penalty”, while other groups of academics are blurred. Even more underinvestigated and not yet fully explained are the intersections between further axes of diversity, often because the research conducted during the pandemic was based on a small volume of in-depth data. By drawing on interview data from a wider national research project, this article aims to contribute to this debate by adopting an intersectional approach. In investigating daily working life and work–life balance during the pandemic of a highly heterogeneous sample of 127 Italian academics, this article sheds light on how gender combines with other axes of asymmetry, particularly class (precarious versus stable and prestigious career positions) and age (individuals’ life-course stage), to produce specific conditions of interrelated (dis)advantage for some academics. The analysis reveals three household and family life course types that embody the interlocking of gender, class, and age within a specific social location with unequal, and possibly long-term, consequences for the quality of working life, well-being, and careers of academics, living alone or with parents, couples without children or with grown-up children, and couples with young children and other family members in need of care.