biotechnology v1
Authors: Murray Nancy 
Published: July 2019

What We Can Learn about Western Blot? Western blot, also known as protein immunoblot,is an analytical technique used to detect specific proteins in a given sample, such as cell lysates, tissue homogenate or extract, which is often used to isolate and identify proteins. It is widely used in molecular biology. Western blot refers to the transfer of proteins separated by gel electrophoresis to membranes and then detecting the proteins on the transfer membrane. As for its time for appearance, the Western blottechnique has grown for decades. Western blot was proposed in 1979 by Harry Towbin from Switzerland. It was first called Western Blot in the Analytical Biochemistry, which was written by Neal Burnette in 1981. The bases of western blot identification are two properties: molecular weight and antibody binding specificity. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The basic principle of western blot are protein electrophoresis andELISA. Electrophoresis is a common method of separating proteins by size, shape, or charge.Proteins are large, electrically charged molecules that can migrate through polyacrylamide gels under the action of an electric field. Like a long-distance runner running on a track, the polyacrylamide molecule in the gel acts like a pebble or other obstacles on the track, which may slow the runner down.Tall, strong athletes are more susceptible to obstacles than short ones, so they run slower. That's why we can isolate the proteins of those runners. As a mature technique in molecular biology, western blot has its own set of commercial reagents. Although western blot has been invented for a long time and has been relatively mature, there are still a steady stream of researchers to conduct further research and analysis on it, which also indicates that western blot is of great research value.Western blot technology has made great progress since its development. However, from the reports on Western blot materials in recent years, it is not difficult to find that the related research is still lagging behind, mainly because the nature of the protein itself is relatively complex, which led to most of the material application value is only limited to the analysis of the biological and chemical fields, and fewer researchers are committed to mining the application of Western blot in other fields.However, with the progress of times and the development and innovation of science and technology, people cannot easily judge the future development prospect of western blot technology. However, according to related research materials, once the development of western blot technology becomes more mature, researchers can try to use its specific recognition function to replace protein or polypeptide targeting cephalic groups in new drug targeting delivery systems.In this way, the application will greatly contribute to the long-term development of molecular biology, and will also leave an indelible contribution to innovative research in the field of biology and chemistry. If biological researchers focus on the development of alternative applications of western blot technology, this technology will not only play a less unique role, but also contribute to the overall trend and development of the biological industry.Of course, the development of western blot technology still has a long way to go. However, with the rapid development of science and technology, I believe that with the unremitting efforts of researchers, they will surely open up a broader application prospect. Now, anything else I can learn? As a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components and critical assay reagents, Creative Diagnostics has built a mature production technology service system for western blot and have perfect SOPs for efficient implementation. As part of the protein experiment, western blothas good technical support, sells well in many places and enjoys a high reputation in the industry. For additional Information. Please contact: Email: contact@creative-diagnostics.com Tel: 631-619-7922 Address: 45-1 Ramsey Road, Shirley, NY 11967, USAResource:https://www.creative-diagnostics.com/The-Basis-of-Western-Blot.htm