bluuebunny/crossref_metadata_2025 huggingface dataset:
Executive Functions of Adults with Binge-Eating Disorder: The Role of Weight Status and Psychopathology
Authors: Busch Nele , Schmidt Ricarda , Hilbert Anja
Published: December 2021

Findings on executive functions (EFs) in binge-eating disorder (BED) are inconsistent and possibly biased by associated comorbidities. This study aimed to identify whether distinct levels of physical and mental comorbidity are related to EFs in BED. General and food-specific EFs in n = 77 adults with BED were compared to population-based norms and associations with weight status, depressive symptoms, and eating disorder psychopathology were analyzed. To detect within-sample patterns of EF performance, k-means clustering was applied. The results indicated that participants’ general EFs were within the average range with slight deficits in alertness. While depression and eating disorder psychopathology were unrelated to EFs, weight status was associated with food-specific attentional bias that was significantly higher in obesity class 2 than in overweight/obesity class 1 and obesity class 3. Four meaningful clusters with distinct strengths and impairments in general and food-specific EFs but without differences in clinical variables were identified. Altogether, adults with BED showed few specific deficits compared to normative data. Performance was unrelated to depression and eating disorder psychopathology, while weight status was associated with food-specific EFs only. The results highlight the need for longitudinal studies to evaluate the relevance of EFs in BED development and maintenance in neurologically healthy adults.
The Role of Dopaminergic Genes in Probabilistic Reinforcement Learning in Schizophrenia Spectrum Disorders
Authors: Frydecka Dorota , Misiak Błażej , Piotrowski Patryk , Bielawski Tomasz , Pawlak Edyta , Kłosińska Ewa , Krefft Maja , Al Noaimy Kamila , Rymaszewska Joanna , Moustafa Ahmed A. , Drapała Jarosław
Published: December 2021

Schizophrenia spectrum disorders (SZ) are characterized by impairments in probabilistic reinforcement learning (RL), which is associated with dopaminergic circuitry encompassing the prefrontal cortex and basal ganglia. However, there are no studies examining dopaminergic genes with respect to probabilistic RL in SZ. Thus, the aim of our study was to examine the impact of dopaminergic genes on performance assessed by the Probabilistic Selection Task (PST) in patients with SZ in comparison to healthy control (HC) subjects. In our study, we included 138 SZ patients and 188 HC participants. Genetic analysis was performed with respect to the following genetic polymorphisms: rs4680 in COMT, rs907094 in DARP-32, rs2734839, rs936461, rs1800497, and rs6277 in DRD2, rs747302 and rs1800955 in DRD4 and rs28363170 and rs2975226 in DAT1 genes. The probabilistic RL task was completed by 59 SZ patients and 95 HC subjects. SZ patients performed significantly worse in acquiring reinforcement contingencies during the task in comparison to HCs. We found no significant association between genetic polymorphisms and RL among SZ patients; however, among HC participants with respect to the DAT1 rs28363170 polymorphism, individuals with 10-allele repeat genotypes performed better in comparison to 9-allele repeat carriers. The present study indicates the relevance of the DAT1 rs28363170 polymorphism in RL in HC participants.
Architectural Significance of the Seokguram Buddhist Grotto in Gyeongju (Korea)
Authors: Salguero-Andújar Francisco , Prat-Hurtado Fulgencio , Rodriguez-Cunill Inmaculada , Cabeza-Lainez Joseph
Published: December 2021

The purpose of this article is to disclose the hidden architectural proportions and true nature of the Korean national treasure in Seokguram Grotto, Gyeongju. The authors compare its features with those of other ancient hypogeal or ashlar constructions with the intention of rediscovering its relevant configuration and latent structural properties in order to demonstrate its uniqueness. The methods employed in the research belong initially to architectural design and composition to advance in the later stages to the nuances of stone masonry, lighting effects and especially cohesive construction. In this discussion and thorough analysis diverse philosophical and scientific subtleties are brought to the surface. The results demonstrate significant potential thanks to recent architectural developments, such as Tadao Ando’s Buddha Hill in Hokkaido (2017) and the authors’ own proposal for a Buddhist monument.
Updates on Molecular Targeted Therapies for Intraparenchymal CNS Metastases
Authors: Sharma Akanksha , Singer Lauren , Kumthekar Priya
Published: December 2021

Central nervous system (CNS) metastases can occur in a high percentage of systemic cancer patients and is a major cause of morbidity and mortality in these patients. Almost any histology can find its way to the brain, but lung, breast, and melanoma are the most common pathologies seen in the CNS from metastatic disease. Identification of many key targets in the tumorigenesis pathway has been crucial to the development of a number of drugs that have demonstrated successful penetration of the blood–brain, blood–cerebrospinal fluid, and blood–tumor barriers. Targeted therapy and immunotherapy have dramatically revolutionized the field with treatment options that can provide successful and durable control of even CNS disease. In this review, we discuss major targets with successful treatment options as demonstrated in clinical trials. These include tyrosine kinase inhibitors, monoclonal antibodies, and antibody–drug conjugates. We also provide an update on the state of the field and highlight key upcoming trials. Patient-specific molecular information combined with novel therapeutic approaches and new agents has demonstrated and continues to promise significant progress in the management of patients with CNS metastases.
Promotion Effect of the Keggin Structure on the Sulfur and Water Resistance of Pt/CeTi Catalysts for CO Oxidation
Authors: Zhang Tong , Qiu Wenge , Zhu Hongtai , Ding Xinlei , Wu Rui , He Hong
Published: December 2021

Developing a catalyst with high SO2 and H2O resistance to achieve high-performance CO oxidation for specific industrial applications is highly desirable. Here, three catalysts were prepared using cerium titanium composite oxide (CeTi), molybdophosphate with Keggin structure-modified CeTi (Keg-CeTi), and molybdophosphate without Keggin structure-modified CeTi (MoP-CeTi) as supports, and their sulfur and water resistance in CO oxidation were tested. The characterization of XRD, BET, SO2/H2O-DRIFTS, XPS, TEM, SEM, NH3/SO2-TPD, H2-TPR, and ICP techniques revealed that the high SO2 and H2O resistance of Pt/Keg-CeTi in CO oxidation was related to its stronger surface acidity, better reduction of surface cerium and molybdenum species, and lower SO2 adsorption and transformation compared to Pt/CeTi and Pt/MoP-CeTi.
In Vitro and In Vivo Models to Study Nephropathic Cystinosis
Authors: Cheung Pang Yuk , Harrison Patrick T. , Davidson Alan J. , Hollywood Jennifer A.
Published: December 2021

The development over the past 50 years of a variety of cell lines and animal models has provided valuable tools to understand the pathophysiology of nephropathic cystinosis. Primary cultures from patient biopsies have been instrumental in determining the primary cause of cystine accumulation in the lysosomes. Immortalised cell lines have been established using different gene constructs and have revealed a wealth of knowledge concerning the molecular mechanisms that underlie cystinosis. More recently, the generation of induced pluripotent stem cells, kidney organoids and tubuloids have helped bridge the gap between in vitro and in vivo model systems. The development of genetically modified mice and rats have made it possible to explore the cystinotic phenotype in an in vivo setting. All of these models have helped shape our understanding of cystinosis and have led to the conclusion that cystine accumulation is not the only pathology that needs targeting in this multisystemic disease. This review provides an overview of the in vitro and in vivo models available to study cystinosis, how well they recapitulate the disease phenotype, and their limitations.
A Next Generation Sequencing-Based Protocol for Screening of Variants of Concern in Autism Spectrum Disorder
Authors: Huang Jie , Liu Jun , Tian Ruiyi , Liu Kevin , Zhuang Patrick , Sherman Hannah Tayla , Budjan Christoph , Fong Michelle , Jeong Min-Seo , Kong Xue-Jun
Published: December 2021

Autism spectrum disorder (ASD) is a neurodevelopmental disorder with strong genetic influences. There is an increasing demand for ASD genetic testing beyond the traditionally recommended microarray and syndromic autism testing; however, the current whole genome sequencing (WGS) and whole exome sequencing (WES) methods are lacking an academic standard for WGS variant annotation, reporting, and interpretation, tailored towards patients with ASD and offer very limited interpretation for clinical significance. Using WGS data from six family trios, we demonstrate the clinical feasibility and technical implementation of an evidence-based, fully transparent bioinformatics pipeline and report framework for an ASD-focused WGS genetic report. We confirmed a portion of the key variants with Sanger sequencing and provided interpretation with consideration of patients’ clinical symptoms and detailed literature review. Furthermore, we showed that identification of the genetic contributions of ASD core symptoms and comorbidities may promote a better understanding of the ASD pathophysiology, lead to early detection of associated comorbidities, and facilitate pharmacologic intervention based on pathological pathways inferred from the genetic information. We will make the bioinformatics pipeline and interpretation framework publicly available, in an easily accessible format, after validation with a larger cohort. We hope that the present proposed protocol can serve as a starting point to invite discourse and debate to further improve approaches in WGS-based genetic consultation for patients with ASD.
The Cytoplasmic Dynein Associated Protein NDE1 Regulates Osteoclastogenesis by Modulating M-CSF and RANKL Signaling Pathways
Authors: Das Bhaba K. , Gogoi Jyoti , Kannan Aarthi , Gao Ling , Xing Weirong , Mohan Subburaman , Zhao Haibo
Published: December 2021

Cytoskeleton organization and lysosome secretion play an essential role in osteoclastogenesis and bone resorption. The cytoplasmic dynein is a molecular motor complex that regulates microtubule dynamics and transportation of cargos/organelles, including lysosomes along the microtubules. LIS1, NDE1, and NDEL1 belong to an evolutionary conserved pathway that regulates dynein functions. Disruption of the cytoplasmic dynein complex and deletion of LIS1 in osteoclast precursors arrest osteoclastogenesis. Nonetheless, the role of NDE1 and NDEL1 in osteoclast biology remains elusive. In this study, we found that knocking-down Nde1 expression by lentiviral transduction of specific shRNAs markedly inhibited osteoclastogenesis in vitro by attenuating the proliferation, survival, and differentiation of osteoclast precursor cells via suppression of signaling pathways downstream of M-CSF and RANKL as well as osteoclast differentiation transcription factor NFATc1. To dissect how NDEL1 regulates osteoclasts and bone homeostasis, we generated Ndel1 conditional knockout mice in myeloid osteoclast precursors (Ndel1ΔlysM) by crossing Ndel1-floxed mice with LysM-Cre mice on C57BL/6J background. The Ndel1ΔlysM mice developed normally. The µCT analysis of distal femurs and in vitro osteoclast differentiation and functional assays in cultures unveiled the similar bone mass in both trabecular and cortical bone compartments as well as intact osteoclastogenesis, cytoskeleton organization, and bone resorption in Ndel1ΔlysM mice and cultures. Therefore, our results reveal a novel role of NDE1 in regulation of osteoclastogenesis and demonstrate that NDEL1 is dispensable for osteoclast differentiation and function.
Myeloid-Derived Suppressor Cells in Prostate Cancer: Present Knowledge and Future Perspectives
Authors: Koinis Filippos , Xagara Anastasia , Chantzara Evangelia , Leontopoulou Vassiliki , Aidarinis Chrissovalantis , Kotsakis Athanasios
Published: December 2021

Several lines of research are being investigated to better understand mechanisms implicated in response or resistance to immune checkpoint blockade in prostate cancer (PCa). Myeloid-derived suppressor cells (MDSCs) have emerged as a major mediator of immunosuppression in the tumor microenvironment that promotes progression of various tumor types. The main mechanisms underlying MDSC-induced immunosuppression are currently being explored and strategies to enhance anti-tumor immune response via MDSC targeting are being tested. However, the role of MDSCs in PCa remains elusive. In this review, we aim to summarize and present the state-of-the-art knowledge on current methodologies to phenotypically and metabolically characterize MDSCs in PCa. We describe how these characteristics may be linked with MDSC function and may influence the clinical outcomes of patients with PCa. Finally, we briefly discuss emerging strategies being employed to therapeutically target MDSCs and potentiate the long-overdue improvement in the efficacy of immunotherapy in patients with PCa.
Cynanchum atratum Alleviates Non-Alcoholic Fatty Liver by Balancing Lipogenesis and Fatty Acid Oxidation in a High-Fat, High-Fructose Diet Mice Model
Authors: Wang Jing-Hua , Hwang Seung-Ju , Lim Dong-Woo , Son Chang-Gue
Published: December 2021

Cynanchum atratum, a medicinal herb, is traditionally used as an antidote, diuretic, and antipyretic in eastern Asia. The current study aimed to investigate the anti-fatty liver capacity of the ethanol extract of Cynanchum atratum (CAE) using a 10-week high-fat, high-fructose diet mouse model. A six-week treatment of CAE (from the fifth week) significantly attenuated the weights of the body, liver, and mesenteric fat without a change in diet intake. CAE also considerably restored the alterations of serum aminotransferases and free fatty acid, fasting blood glucose, serum and hepatic triglyceride, and total cholesterol, as well as platelet and leukocyte counts. Meanwhile, CAE ameliorated hepatic injury and lipid accumulation, as evidenced by histopathological and immunofluorescence observations. Additionally, CAE significantly lowered the elevation of hepatic TNF-α, the TNF-α/IL-10 ratio, fecal endotoxins, and the abundance of Gram-negative bacteria. Hepatic lipogenesis and β-oxidation-related proteins and gene expression, including PPAR-α, SREBP-1, SIRT1, FAS, CTP1, etc., were normalized markedly by CAE. In particular, the AMPK, a central regulator of energy metabolism, was phosphorylated by CAE at an even higher rate than metformin. Overall, CAE exerts anti-hepatic steatosis effects by reducing lipogenesis and enhancing fatty acid oxidation. Consequently, Cynanchum atratum is expected to be a promising candidate for treating chronic metabolic diseases.
Fluorescent Chemosensors Based on Polyamine Ligands: A Review
Authors: Verdejo Begoña , Inclán Mario , Clares María Paz , Bonastre-Sabater Irene , Ruiz-Gasent Mireia , García-España Enrique
Published: December 2021

Polyamine ligands are water-soluble receptors that are able to coordinate, depending on their protonation degree, either metal ions, anionic, or neutral species. Furthermore, the presence of fluorescent signaling units allows an immediate visual response/signal. For these reasons, they can find applications in a wide variety of fields, mainly those where aqueous media is necessary, such as biological studies, wastewater analysis, soil contamination, etc. This review provides an overview of the recent developments in the research of chemosensors based on polyamine ligands functionalized with fluorescent signaling units. The discussion focuses on the design, synthesis, and physicochemical properties of this type of fluorescent chemosensors in order to analyze the applications associated to the sensing of metal ions, anions, and neutral molecules of environmental and/or biological interest. To facilitate a quick access and overview of all the chemosensors covered in this review, a summary table of the chemosensor structures and analytes, with all the corresponding references, is also presented.
Optical and Recombination Parameters of CdS1−xTex Thin Films Obtained by the CMBD Method
Authors: Razykov Takhir M. , Patryn Aleksy , Maliński Mirosław , Bychto Leszek , Ergashev Bobur , Kouchkarov Kudratulia , Shukurov Akbarjon , Makhmudov Mirzavkiy , Isaqov Diyorbek
Published: December 2021

This paper presents the results of the photoacoustic, SEM, and surface photovoltage experiments performed on the series of CdS1−xTex thin films. These CdS1−xTex (0 ≤ x ≤ 1) thin films were obtained on the glass substrate by the chemical molecular beam deposition (CMBD) method. The polycrystalline character of these films was revealed by SEM pictures. From the experimental optical characteristics, the optical absorption coefficient spectra of the samples and values of their energy gaps vs. their composition were determined. From the surface photovoltage characteristics, the diffusion lengths of the carriers were also determined.
Solution-Processable Growth and Characterization of Dandelion-like ZnO:B Microflower Structures
Authors: Erat Selma , Braun Artur , Çetinkaya Samed , Yildirimcan Saadet , Kasapoğlu Ahmet Emre , Gür Emre , Harputlu Ersan , Ocakoglu Kasım
Published: December 2021

Intrinsic and dandelion-like microflower nano-rod structures of boron-doped ZnO thin films were synthesized with an ecofriendly and cost-effective chemical bath deposition technique from an aqueous solution of zinc nitrate hexahdyrate [Zn(NO3)2.6H2O] as a precursor solution and boric acid as a doping solution. The boron concentrations were 0.1, 0.3, 0.5, 1.0, 3.0, 5.0, and 7.0 by volume. Scanning electron micrographs showed that doping with boron appears to hinder the vertical alignment of crystallites. Additionally, independent hexagonal nano-rod structures were observed to coalesce together to form dandelion-like structures on the film’s surface. The atomic ratio of the elements was determined via the X-ray photoemission spectrum technique. There were no substantial changes in the vibration structure of the film upon doping in terms of the Raman spectra. The optical band gap of ZnO (3.28 eV) decreased with B doping. The band gap of the ZnO:B film varied between 3.18 and 3.22 eV. The activation energy of the ZnO was calculated as 0.051 eV, whereas that of the ZnO:B film containing 1.0% B was calculated as 0.013 eV at low temperatures (273–348 K), versus 0.072 eV and 0.183 eV at high temperatures (348–523 K), respectively. Consequently, it can be interpreted that the 1% B-doped ZnO, which has the lowest activation energy at both low and high temperatures, may find some application areas such as in sensors for gases and in solar cells.
Self-Localized Liquid Crystal Micro-Droplet Arrays on Chemically Patterned Surfaces
Authors: Kołacz Jakub , Wei Qi-Huo
Published: December 2021

Liquid crystal (LC) micro-droplet arrays are elegant systems that have a range of applications, such as chemical and biological sensing, due to a sensitivity to changes in surface properties and strong optical activity. In this work, we utilize self-assembled monolayers (SAMs) to chemically micro-pattern surfaces with preferred regions for LC occupation. Exploiting discontinuous dewetting, dragging a drop of fluid over the patterned surfaces demonstrates a novel, high-yield method of confining LC in chemically defined regions. The broad applicability of this method is demonstrated by varying the size and LC phase of the droplets. Although the optical textures of the droplets are dictated by topological constraints, the additional SAM interface is shown to lock in inhomogeneous alignment. The surface effects are highly dependent on size, where larger droplets exhibit asymmetric director configurations in nematic droplets and highly knotted structures in cholesteric droplets.
Depicting the DNA Binding and Cytotoxicity Studies against Human Colorectal Cancer of Aquabis (1-Formyl-2-Naphtholato-k2O,O′) Copper(II): A Biophysical and Molecular Docking Perspective
Authors: Alolayqi Ebtisam , Afzal Mohd , Alarifi Abdullah , Beagan Abeer , Muddassir Mohd
Published: December 2021

In this study, we attempted to examine the biological activity of the copper(II)–based small molecule aquabis (1-formyl-2-naphtholato-k2O,O′)copper(II) (1) against colon cancer. The characterization of complex 1 was established by analytical and spectral methods in accordance with the single-crystal X-ray results. A monomeric unit of complex 1 exists in an O4 (H2O) coordination environment with slightly distorted square pyramidal geometry (τ = ~0.1). The interaction of complex 1 with calf thymus DNA (ctDNA) was determined by employing various biophysical techniques, which revealed that complex 1 binds to ctDNA at the minor groove with a binding constant of 2.38 × 105 M–1. The cytotoxicity of complex 1 towards human colorectal cell line (HCT116) was evaluated by the MTT assay, which showed an IC50 value of 11.6 μM after treatment with complex 1 for 24 h. Furthermore, the apoptotic effect induced by complex 1 was validated by DNA fragmentation pattern, which clarified that apoptosis might be regulated through the mitochondrial-mediated production of reactive oxygen species (ROS) causing DNA damage pathway. Additionally, molecular docking was also carried out to confirm the recognition of complex 1 at the minor groove.
Lymphatic Endothelial Markers and Tumor Lymphangiogenesis Assessment in Human Breast Cancer
Authors: Chen Jia-Mei , Luo Bo , Ma Ru , Luo Xi-Xi , Chen Yong-Shun , Li Yan
Published: December 2021

Metastasis via lymphatic vessels or blood vessels is the leading cause of death for breast cancer, and lymphangiogenesis and angiogenesis are critical prerequisites for the tumor invasion–metastasis cascade. The research progress for tumor lymphangiogenesis has tended to lag behind that for angiogenesis due to the lack of specific markers. With the discovery of lymphatic endothelial cell (LEC) markers, growing evidence demonstrates that the LEC plays an active role in lymphatic formation and remodeling, tumor cell growth, invasion and intravasation, tumor–microenvironment remodeling, and antitumor immunity. However, some studies have drawn controversial conclusions due to the variation in the LEC markers and lymphangiogenesis assessments used. In this study, we review recent findings on tumor lymphangiogenesis, the most commonly used LEC markers, and parameters for lymphangiogenesis assessments, such as the lymphatic vessel density and lymphatic vessel invasion in human breast cancer. An in-depth understanding of tumor lymphangiogenesis and LEC markers can help to illustrate the mechanisms and distinct roles of lymphangiogenesis in breast cancer progression, which will help in exploring novel potential predictive biomarkers and therapeutic targets for breast cancer.
Menopausal Status Combined with Serum CA125 Level Significantly Predicted Concurrent Endometrial Cancer in Women Diagnosed with Atypical Endometrial Hyperplasia before Surgery †
Authors: Lou Yaochen , Liao Jiongbo , Shan Weiwei , Xu Zhiying , Chen Xiaojun , Guan Jun
Published: December 2021

About 10–66% of patients with atypical endometrial hyperplasia diagnosed before surgery (preoperative-AEH) are found to have concurrent endometrial cancer (EC) at definitive hysterectomy, leading to incomplete primary surgery and delayed adjuvant treatment. This study aims to investigate the potential risk factors of concurrent EC in preoperative-AEH patients in a clinical setting with a gynecological pathology review. All patients diagnosed with AEH by endometrial biopsy or curettage that then underwent definitive hysterectomy from January 2016 to December 2019 in a tertiary hospital were retrospectively analyzed. All diagnoses were reviewed by gynecological pathologists. A total of 624 preoperative-AEH patients were included, 30.4% of whom had concurrent EC. In multivariate analysis, postmenopausal status and CA125 ≥ 35 U/mL significantly correlated with concurrent EC (OR = 3.57; 95% CI = 1.80–7.06; OR = 2.15; 95% CI = 1.15–4.03). This risk was remarkably increased in patients with both postmenopausal status and CA125 ≥ 35 U/mL (OR = 16.20; 95% CI = 1.73–151.44). Notably, concurrent EC seemed to occur more frequently in women with postmenopausal time ≥ 5 years (OR = 4.04, 95% CI = 1.80–5.85). In addition, CA125 ≥ 35 U/mL seemed to be an independent risk factor (OR = 5.74; 95% CI = 1.80–18.27) for concurrent intermediate-high-risk EC. Intermediate-high-risk EC was also more commonly seen in preoperative-AEH women with postmenopausal time ≥ 5 years (OR = 5.52, 95% CI = 1.21–25.19, p = 0.027). In conclusion, preoperative-AEH patients with postmenopausal status or elevated level of CA125 might have a high risk of concurrent EC. Adequate pre-surgical evaluation might be suggested for such patients.
RT-PCR Detection of SARS-CoV-2 among Individuals from the Upper Silesian Region—Analysis of 108,516 Tests
Authors: Konka Adam , Lejawa Mateusz , Gaździcka Jadwiga , Bochenek Aneta , Fronczek Martyna , Strzelczyk Joanna Katarzyna
Published: December 2021

Background: The COVID-19 pandemic triggered by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has left a huge mark on everyday lives, introducing restrictions and plunging the global economy. This study aimed to analyze the available epidemiological data from the register of one of the largest laboratories testing for SARS-CoV-2 in the Silesian voivodship of Poland. Methods: This analysis is based upon the epidemiological records collected between 30 March 2020, and 30 April 2021, by the Silesian Park of Medical Technology Kardio-Med Silesia (Zabrze, Poland). In addition, we performed SARS-CoV-2 variant detection in samples from patients reinfected with SARS-CoV-2. Results: Our results confirm that SARS-CoV-2 infections are more common in urban areas. Laboratory-confirmed COVID-19 cases represent 13.21% of all RT-PCR test results during the 13 months of our laboratory diagnostics for SARS-CoV-2 infections. Detection of SARS-CoV-2 variants in samples of potentially reinfected patients showed discrepancies in the results. Conclusions: Due to the higher risk of SARS-CoV-2 infection among the Upper Silesian population, the region is at greater risk of deteriorating economic situation and healthcare as compared to other areas of Poland. RT-PCR methods are inexpensive and suitable for large-scale screening, but they can be untrustworthy so detection of SARS-CoV-2 variants in samples should be confirmed by sequencing.
Tourism Demand Elasticities by Income and Prices of International Market Regions: Evidence Using Vietnam’s Data
Authors: Nguyen Quang Hai
Published: December 2021

This study aims to determine tourism demand elasticities by income and prices of regions, as well as total international markets. It is deployed to a total of 10 major source markets and two separate regions, from Asia and intercontinental to Vietnam. The results of data analysis for the period 1995–2019 and using a nonlinear panel ARDL approach show that tourism demand from major Asian markets to Vietnam is strongly income elastic, but tourism demand from major intercontinental markets to Vietnam is relatively price inelastic. Tourism demand in intercontinental markets is less elastic to price, but in Asian markets it is quite price sensitive, especially own price elasticities. In addition, different effects of income and prices are found in most of the major markets. Study results have provided useful insights into different types of tourism goods and price sensitivity between market regions, as well as the degree of substitute destinations.
Computer Science beyond Coding: Partnering to Create Teacher Cybersecurity Microcredentials
Authors: Burrows Andrea C. , Borowczak Mike , Mugayitoglu Bekir
Published: December 2021

Computer science, cybersecurity education, and microcredentials are becoming more pervasive in all levels of the educational system. The purpose of this study was partnering with precollegiate teachers: (1) to investigate the self-efficacy of 30 precollegiate teacher participants towards computer science before, during, and after three iterations of a cybersecurity microcredential, and (2) to make changes to the cybersecurity microcredential to improve its effectiveness. The authors explored what teachers need in a microcredential. The first Cohort (n = 5) took the microcredential sequence over 28 days in the summer of 2020, the second Cohort (n = 16) took it over 42 days in the fall of 2020, and the third Cohort (n = 9) took it over 49 days in the summer of 2021. The authors investigated three research questions and used a systems thinking approach while developing, evaluating, and implementing the research study. The researchers used quantitative methods in the collection of a self-efficacy subscale survey to assess whether the precollegiate teachers’ beliefs about computer science changed, and then used qualitative methods when conducting semi-structured teacher participant interviews to address the research questions. The findings show that the precollegiate teachers’ self-efficacy scores towards computer science increased, and that there are areas in need of attention, such as resources and implementation, when creating microcredentials. The implications of this research include the importance of purposefully crafting microcredentials and professional developments, including aspects of creating effective partnerships.
Optimized Operation of Integrated Energy Microgrid with Energy Storage Based on Short-Term Load Forecasting
Authors: Dong Hanlin , Fang Zhijian , Ibrahim Al-wesabi , Cai Jie
Published: December 2021

This research proposes an optimization technique for an integrated energy system that includes an accurate prediction model and various energy storage forms to increase load forecast accuracy and coordinated control of various energies in the current integrated energy system. An artificial neural network is utilized to create an accurate short-term load forecasting model to effectively predict user demand. The 0–1 mixed integer linear programming approach is used to analyze the optimal control strategy for multiple energy systems with storage, cold energy, heat energy, and electricity to solve the problem of optimal coordination. Simultaneously, a precise load forecasting method and an optimal scheduling strategy for multienergy systems are proposed. The equipment scheduling plan of the integrated energy system of gas, heat, cold, and electricity is proposed after researching the operation characteristics and energy use process of the equipment in the combined power supply system. A system economic operation model is created with profit maximization in mind, while also taking into account energy coordination between energy and the power grid. The rationality of the algorithm and model is verified by analyzing the real data of a distributed energy station in Wuhan for two years.
Peak Shaving and Frequency Regulation Coordinated Output Optimization Based on Improving Economy of Energy Storage
Authors: Liu Daobing , Jin Zitong , Chen Huayue , Cao Hongji , Yuan Ye , Fan Yu , Song Yingjie
Published: December 2021

In this paper, a peak shaving and frequency regulation coordinated output strategy based on the existing energy storage is proposed to improve the economic problem of energy storage development and increase the economic benefits of energy storage in industrial parks. In the proposed strategy, the profit and cost models of peak shaving and frequency regulation are first established. Second, the benefits brought by the output of energy storage, degradation cost and operation and maintenance costs are considered to establish an economic optimization model, which is used to realize the division of peak shaving and frequency regulation capacity of energy storage based on peak shaving and frequency regulation output optimization. Finally, the intra-day model predictive control method is employed for rolling optimization. An intra-day peak shaving and frequency regulation coordinated output optimization strategy of energy storage is proposed. Through the example simulation, the experiment results show that the electricity cost of the whole day is reduced by 10.96% by using the coordinated output strategy of peak shaving and frequency regulation. The obtained further comparative analysis results and the life cycle economic analysis show that the profit brought by the proposed coordinated output optimization strategy is greater than that for separate peak shaving or frequency modulation of energy storage under the same capacity.
Inorganic Geochemistry of Crude Oils of Northern Eurasia after ICP-MS Data as Clear Evidence for Their Deep Origin
Authors: Ivanov Kirill Svyatoslavich , Erokhin Yuriy Viktorovich , Kudryavtsev Daniil Aleksandrovich
Published: December 2021

The emergence of mass-spectroscopy with inductively-coupled plasma (ICP-MS) made it possible to study the microelement composition of crude oil and its derivatives with the limit of detection (LOD) at the ppt level. We have studied the crudes from West Siberian (Shaimsky, Sredneobsky and Oktyabrsky regions) and Tatarstan Romashinskoye oilfields with the ICP-MS method to detect 50 rare, rare-earth, and other microelements. The elemental composition is reasonably comparable to their concentrations in ultrabasites whereas the contents of most of the elements are low to the limit. On the diagrams of rare-earth elements, one can see the prevalence of light lanthanides and positive europium anomaly. Increased content of platinoids was found in Tatar oils; in some cases, the concentration of ruthenium reaches 0.06 g/t. The study shows that studied crudes have a specific microelement composition. Based on the elevated contents of transit elements and platinoids, a conclusion was made about the “ultrabasic” geochemical–metallogenic specialization of studied petroleum systems and the assumption about its origin was proposed.
Impact of Forest Fires on Air Quality in Wolgan Valley, New South Wales, Australia—A Mapping and Monitoring Study Using Google Earth Engine
Authors: Singh Sachchidanand , Singh Harikesh , Sharma Vishal , Shrivastava Vaibhav , Kumar Pankaj , Kanga Shruti , Sahu Netrananda , Meraj Gowhar , Farooq Majid , Singh Suraj Kumar
Published: December 2021

Forests are an important natural resource and are instrumental in sustaining environmental sustainability. Burning biomass in forests results in greenhouse gas emissions, many of which are long-lived. Precise and consistent broad-scale monitoring of fire intensity is a valuable tool for analyzing climate and ecological changes related to fire. Remote sensing and geographic information systems provide an opportunity to improve current practice’s accuracy and performance. Spectral indices techniques such as normalized burn ratio (NBR) have been used to identify burned areas utilizing satellite data, which aid in distinguishing burnt areas using their standard spectral responses. For this research, we created a split-panel web-based Google Earth Engine app for the geo-visualization of the region severely affected by forest fire using Sentinel 2 weekly composites. Then, we classified the burn severity in areas affected by forest fires in Wolgan Valley, New South Wales, Australia, and the surrounding area through Difference Normalized Burn Ratio (dNBR). The result revealed that the region’s burnt area increased to 6731 sq. km in December. We also assessed the impact of long-term rainfall and land surface temperature (LST) trends over the study region to justify such incidents. We further estimated the effect of such incidents on air quality by analyzing the changes in the column number density of carbon monoxide and nitrogen oxides. The result showed a significant increase of about 272% for Carbon monoxide and 45% for nitrogen oxides. We conclude that, despite fieldwork constraints, the usage of different NBR and web-based application platforms may be highly useful for forest management to consider the propagation of fire regimes.
Spider Assemblages of Tree Branches in Managed and Primeval Deciduous Stands of the Białowieża Forest
Authors: Stańska Marzena , Stański Tomasz , Bartos Maciej
Published: December 2021

The Białowieża Forest is an important biodiversity hotspot on a European scale, and therefore its preservation should have a high priority. However, forest management conducted over a large area of the forest, intensive logging, and elimination of dead trees pose serious threats to many species in the forest. The main aim of this study was to determine the species composition of spider assemblages inhabiting tree branches of the Białowieża Forest and to compare their species richness and the abundance of individuals (adults and juveniles) between managed and primeval stands. Between April and November 2000, we sampled three forest types (oak–lime–hornbeam forest, ash–alder riparian forest, and alder carr) in protected primeval stands within the Białowieża National Park and in managed stands. We collected 1761 specimens from 14 families and identified 41 species. Tree branches were inhabited mainly by juveniles. Species richness was smaller in managed stands compared to primeval stands. The highest number of species was found in primeval alder carr. Our study shows a negative effect of forest management on spider assemblages in terms of species richness. We emphasize the important role of alder carr forests as potential biodiversity hotspots.
Differences in Airborne Particulate Matter Concentration in Urban Green Spaces with Different Spatial Structures in Xi’an, China
Authors: Jiang Bo , Sun Chang , Mu Sen , Zhao Zixin , Chen Yingyuan , Lin Yiwei , Qiu Ling , Gao Tian
Published: December 2021

With the acceleration of urbanization and industrialization, air pollution is becoming one of the most serious problems in cities. Urban green spaces, as “green infrastructure”, are an important part of urban ecosystems for air purification. Therefore, 10 typical green spaces of urban parks in the city of Xi’an, China, were selected as study areas according to vegetation structure and species composition. Considering meteorological factors and time changes, the effects of the selected green spaces with different vegetation structures of different heights on the reduction in airborne particulate matter concentration were explored. The results showed that the following: (1) Temperature, relative humidity, wind speed, and air pressure had significant correlation with the concentration of airborne particulate matter at the different heights, and the correlations were the same at 1.5 m and 5 m. (2) After heating in winter, the concentration of airborne particulate matter with different particle sizes increased significantly. The concentration of airborne particulate matter showed different trends throughout the day, and the small particles (PM1 and PM2.5) had a trend of “lower in the morning and evening, and higher at noon”, while the large particles (PM10 and TSP) gradually decreased over time. (3) In the selected green spaces with different vegetation structure types, the concentration of airborne particulate matter below the canopy (1.5 m) was generally higher than that in the middle of the canopy (5 m), but the effects of reducing the concentration of airborne particulate matter were consistent at the different heights. (4) The adsorption capacity of PM1 and PM2.5 concentration was strong in the partially closed broad-leaved one-layered forest (PBO), and poor in the partially closed broad-leaved multi-layered forest (PBM). Partially closed broad-leaved multi-layered forest (PBM) and partially closed coniferous and broad-leaved mixed multi-layered forest (PMM) also had strong dust-retention effect on PM10 and TSP, while closed broad-leaved one-layered forest (CBO) had a poor dust-retention effect. The results showed that the reduction effects of urban green spaces with different spatial structures on air particles were different, and were restricted by various environmental factors, which could provide a theoretical basis for the optimization of urban green space structure and the improvement of urban air quality.
Velocity Field Measurements of the California Sea Lion Propulsive Stroke Using Bubble PIV
Authors: Perrotta Gino , Fish Frank E. , Adams Danielle S. , Leahy Ariel M. , Downs Abigal M. , Leftwich Megan C.
Published: December 2021

California sea lions are among the most agile of swimming mammals. Most marine mammals swim with their hind appendages—flippers or flukes, depending on the species—whereas sea lions use their foreflippers for propulsion and maneuvering. The sea lion’s propulsive stroke generates thrust by forming a jet between the flippers and the body and by dragging a starting vortex along the suction side of the flipper. Prior experiments using robotic flippers have shown these mechanisms to be possible, but no flow measurements around live sea lions previously existed with which to compare. In this study, the flow structures around swimming sea lions were observed using an adaptation of particle imaging velocimetry. To accommodate the animals, it was necessary to use bubbles as seed particles and sunlight for illumination. Three trained adult California sea lions were guided to swim through an approximately planar sheet of bubbles in a total of 173 repetitions. The captured videos were used to calculate bubble velocities, which were processed to isolate and inspect the flow velocities caused by the swimming sea lion. The methodology will be discussed, and measured flow velocities will be presented.
Phenolics Profile, Antioxidant Activity and Flavor Volatiles of Pear Juice: Influence of Lactic Acid Fermentation Using Three Lactobacillus Strains in Monoculture and Binary Mixture
Authors: Wang Leyu , Zhang Hexin , Lei Hongjie
Published: December 2021

The aim of this study was to evaluate the effects of lactic acid fermentation using three Lactobacillus strains (Lactiplantibacillus plantarum 90, Lactobacillus helveticus 76, and Lacticaseibacillus casei 37) in monoculture and binary mixture on phenolics profile, antioxidant activity and flavor volatiles in pear juice. Results showed that the colony counts of binary mixture were higher than monoculture in fermented pear juice. The total content of phenols was increased, while that of flavonoids was decreased significantly during fermentation (p < 0.05). Antioxidant activities in fermented peer juice including DPPH and ABTS radical scavenging abilities and ferric reducing antioxidant power (FRAP) were significantly improved (p < 0.05). Binary mixture of Lactiplantibacillus plantarum 90 and Lacticaseibacillus casei 37 fermentation exhibited strong DPPH radical scavenging ability, due to the increase in vanillic acid and arbutin contents. Furthermore, lactic acid fermentation improved the formation of alcohols, esters, acids and terpenoids, and reduced the contents of aldehydes and ketones. Thirty new compounds including 15 alcohols, seven esters, five acids, and three terpenoids were observed in fermented pear juice. Hierarchical cluster revealed that flavor volatiles in pear juice were improved dramatically by Lactobacillus strains fermentation, and there were dramatic differences between monoculture and binary mixture.
Does the Food Ingredient Pectin Provide a Risk for Patients Allergic to Non-Specific Lipid-Transfer Proteins?
Authors: Steigerwald Hanna , Blanco-Perez Frank , Albrecht Melanie , Bender Caroline , Wangorsch Andrea , Endreß Hans-Ulrich , Bunzel Mirko , Mayorga Cristobalina , Torres Maria José , Scheurer Stephan , Vieths Stefan
Published: December 2021

Pectin, a dietary fiber, is a polysaccharide that is widely used in food industry as a gelling agent. In addition, prebiotic and beneficial immunomodulatory effects of pectin have been demonstrated, leading to increased importance as food supplement. However, as cases of anaphylactic reactions after consumption of pectin-supplemented foods have been reported, the present study aims to evaluate the allergy risk of pectin. This is of particular importance since most of the pectin used in the food industry is extracted from citrus or apple pomace. Both contain several allergens such as non-specific lipid transfer proteins (nsLTPs), known to induce severe allergic reactions, which could impair the use of pectins in nsLTP allergic patients. Therefore, the present study for the first time was performed to analyze residual nsLTP content in two commercial pectins using different detection methods. Results showed the analytical sensitivity was diminished by the pectin structure. Finally, spiking of pectin with allergenic peach nsLTP Pru p 3 led to the conclusion that the potential residual allergen content in both pectins is below the threshold to induce anaphylactic reactions in nsLTP-allergic patients. This data suggests that consumption of the investigated commercial pectin products provides no risk for inducing severe reactions in nsLTP-allergic patients.
Assessment of Human Mycotoxin Exposure in Hungary by Urinary Biomarker Determination and the Uncertainties of the Exposure Calculation: A Case Study
Authors: Szabó-Fodor Judit , Szeitzné-Szabó Mária , Bóta Brigitta , Schieszl Tamás , Angeli Cserne , Gambacorta Lucia , Solfrizzo Michele , Szabó András , Kovács Melinda
Published: December 2021

Urinary biomarkers of mycotoxin exposure were evaluated in the case of healthy people (n = 41) and coeliac patients (n = 19) by using a multi-biomarker LC-MS/MS immunoaffinity based method capable to analyse biomarkers of nine mycotoxins, i.e., fumonisin B1 (FB1), fumonisin B2 (FB2), deoxynivalenol (DON), zearalenone (ZEN), ochratoxin A (OTA), Aflatoxin B1 (AFB1), T-2 toxin, HT-2 toxin and Nivalenol (NIV). Urinary biomarker concentrations were used to calculate the probable daily intake (PDI) of fumonisin B1, deoxynivalenol, zearalenone and ochratoxin A and compared with their tolerable daily intake (TDI). The human urinary excretion rate values reported in the literature and the 24 h excretion rate measured in piglets were used to estimate and compare the PDI values of the four mycotoxins. The highest mean biomarker concentrations were found for DON (2.30 ng/mL for healthy people and 2.68 ng/mL for coeliac patients). Mean OTA concentration was significantly higher (p < 0.001) in healthy people compared to coeliac patients. PDI calculated with piglets excretion data exceeded the TDI values by a much smaller percentage than when they were calculated from human data, especially for FB1. The uncertainties arising from the different calculations can be well perceived on the basis of these data.