bluuebunny/crossref_metadata_2025 huggingface dataset:
Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation
Authors: Cicova Zdenka , Dejung Mario , Skalicky Tomas , Eisenhuth Nicole , Hanselmann Steffen , Morriswood Brooke , Figueiredo Luisa M. , Butter Falk , Janzen Christian J.
Published: October 2016

Trypanosomes are masters of adaptation to different host environments during their complex life cycle. Large-scale proteomic approaches provide information on changes at the cellular level, and in a systematic way. However, detailed work on single components is necessary to understand the adaptation mechanisms on a molecular level. Here, we have performed a detailed characterization of a bloodstream form (BSF) stage-specific putative flagellar host adaptation factor Tb927.11.2400, identified previously in a SILAC-based comparative proteome study. Tb927.11.2400 shares 38% amino acid identity with TbFlabarin (Tb927.11.2410), a procyclic form (PCF) stage-specific flagellar BAR domain protein. We named Tb927.11.2400 TbFlabarin-like (TbFlabarinL), and demonstrate that it originates from a gene duplication event, which occurred in the African trypanosomes. TbFlabarinL is not essential for the growth of the parasites under cell culture conditions and it is dispensable for developmental differentiation from BSF to the PCF in vitro. We generated TbFlabarinL-specific antibodies, and showed that it localizes in the flagellum. Co-immunoprecipitation experiments together with a biochemical cell fractionation suggest a dual association of TbFlabarinL with the flagellar membrane and the components of the paraflagellar rod.
Comparative venomics of Psyttalia lounsburyi and P. concolor, two olive fruit fly parasitoids: a hypothetical role for a GH1 β-glucosidase
Authors: Mathé-Hubert Hugo , Colinet Dominique , Deleury Emeline , Belghazi Maya , Ravallec Marc , Poulain Julie , Dossat Carole , Poirié Marylène , Gatti Jean-Luc
Published: October 2016

Venom composition of parasitoid wasps attracts increasing interest – notably molecules ensuring parasitism success on arthropod pests – but its variation within and among taxa is not yet understood. We have identified here the main venom proteins of two braconid wasps, Psyttalia lounsburyi (two strains from South Africa and Kenya) and P. concolor, olive fruit fly parasitoids that differ in host range. Among the shared abundant proteins, we found a GH1 β-glucosidase and a family of leucine-rich repeat (LRR) proteins. Olive is extremely rich in glycoside compounds that are hydrolyzed by β-glucosidases into defensive toxic products in response to phytophagous insect attacks. Assuming that Psyttalia host larvae sequester ingested glycosides, the injected venom GH1 β-glucosidase could induce the release of toxic compounds, thus participating in parasitism success by weakening the host. Venom LRR proteins are similar to truncated Toll-like receptors and may possibly scavenge the host immunity. The abundance of one of these LRR proteins in the venom of only one of the two P. lounsburyi strains evidences intraspecific variation in venom composition. Altogether, venom intra- and inter-specific variation in Psyttalia spp. were much lower than previously reported in the Leptopilina genus (Figitidae), suggesting it might depend upon the parasitoid taxa.
Variation in structure of proteins by adjusting reactive oxygen and nitrogen species generated from dielectric barrier discharge jet
Authors: Park Ji Hoon , Kim Minsup , Shiratani Masaharu , Cho Art. E. , Choi Eun Ha , Attri Pankaj
Published: October 2016

Over the last few years, the variation in liquid chemistry due to the development of radicals generated by cold atmospheric plasma (CAP) has played an important role in plasma medicine. CAP direct treatment or CAP activated media treatment in cancer cells shows promising anticancer activity for both in vivo and in vitro studies. However, the anticancer activity or antimicrobial activity varies between plasma devices due to the different abilities among plasma devices to generate the reactive oxygen and nitrogen species (RONS) at different ratios and in different concentrations. While the generation of RONS depends on many factors, the feeding gas plays the most important role among the factors. Hence, in this study we used different compositions of feeding gas while fixing all other plasma characteristics. We used Ar, Ar-O2 (at different ratios), and Ar-N2 (at different ratios) as the working gases for CAP and investigated the structural changes in proteins (Hemoglobin (Hb) and Myoglobin (Mb)). We then analyzed the influence of RONS generated in liquid on the conformations of proteins. Additionally, to determine the influence of H2O2 on the Hb and Mb structures, we used molecular dynamic simulation.
Crystal structures and inhibition of Trypanosoma brucei hypoxanthine–guanine phosphoribosyltransferase
Authors: Terán David , Hocková Dana , Česnek Michal , Zíková Alena , Naesens Lieve , Keough Dianne T. , Guddat Luke W.
Published: October 2016

Human African Trypanosomiasis (HAT) is a life-threatening infectious disease caused by the protozoan parasite, Trypanosoma brucei (Tbr). Due to the debilitating side effects of the current therapeutics and the emergence of resistance to these drugs, new medications for this disease need to be developed. One potential new drug target is 6-oxopurine phosphoribosyltransferase (PRT), an enzyme central to the purine salvage pathway and whose activity is critical for the production of the nucleotides (GMP and IMP) required for DNA/RNA synthesis within this protozoan parasite. Here, the first crystal structures of this enzyme have been determined, these in complex with GMP and IMP and with three acyclic nucleoside phosphonate (ANP) inhibitors. The Ki values for GMP and IMP are 30.5 μM and 77 μM, respectively. Two of the ANPs have Ki values considerably lower than for the nucleotides, 2.3 μM (with guanine as base) and 15.8 μM (with hypoxanthine as base). The crystal structures show that when two of the ANPs bind, they induce an unusual conformation change to the loop where the reaction product, pyrophosphate, is expected to bind. This and other structural differences between the Tbr and human enzymes suggest selective inhibitors for the Tbr enzyme can be designed.
Plasma Ang2 and ADAM17 levels are elevated during clinical malaria; Ang2 level correlates with severity and expression of EPCR-binding PfEMP1
Authors: Petersen Jens E. V. , Mkumbaye Sixbert I. , Vaaben Anna V. , Manjurano Alphaxard , Lyimo Eric , Kavishe Reginald A. , Mwakalinga Steven B. , Mosha Jacklin , Minja Daniel T. R. , Lusingu John P. A. , Theander Thor G. , Lavstsen Thomas , Wang Christian W.
Published: October 2016

The pathogenesis ofPlasmodium falciparummalaria involves a complex interplay between parasite adhesion and inflammatory response that includes release of cytokines and activation of the endothelium with accompanying release of Angiopoitin 2 (Ang2) to the plasma. A-disintegrin and metalloproteinase 17 (ADAM17) is a protein responsible for releasing cytokines, including Tumor Necrosis Factor α (TNFα), and shedding of adhesion proteins. In this study, we show that plasma levels of ADAM17 are increased in Tanzanian children hospitalized with a malaria infection compared with asymptomatic children but similar to children hospitalized with other infectious diseases. The plasma levels of ADAM17 decreased during recovery after an acute malaria episode. Plasma levels of Ang2 were associated with markers of malaria severity and levels ofvartranscripts encodingP. falciparumErythrocyte Membrane Protein 1 (PfEMP1) containing Cysteine Rich Inter Domain Region α1 (CIDRα1) domains predicted to bind Endothelial Protein C receptor (EPCR). ADAM17 levels were not associated with expression ofvargenes encoding different PfEMP1 types when controlling for age. These data are the first to report ADAM17 plasma levels in malaria-exposed individuals, and support the notion that parasite sequestration mediated by EPCR-binding PfEMP1 is associated with endothelial activation and pathology in severe paediatric malaria.
One compound of saponins from Disocorea zingiberensis protected against experimental acute pancreatitis by preventing mitochondria-mediated necrosis
Authors: Zhang Rui , Wen Li , Shen Yan , Shi Na , Xing Zhihua , Xia Qing , Niu Hai , Huang Wen
Published: October 2016

Acute pancreatitis (AP) is a painful inflammatory disorder of the exocrine pancreas, ranking as the most common gastrointestinal reasons for hospitalization with no specific therapy currently. Diosgenyl saponins extracted from natural products and diosgenin or its derivatives have been shown to exert anti-inflammatory effects in various diseases. However, the therapeutic effects of diosgenyl saponins from Dioscorea zingiberensis C. H. Wright in AP have not yet been determined. Five compounds were extracted and screened for taurocholate-induced necrosis in mouse pancreatic acinar cells. Particularly, 26-O-β-d-glucopyranosyl-3β, 22α, 26-trihydroxy-25(R)-furosta-5-en-3-O-[α-L-rhamnopyranosyl-(1 → 4)]-β-d-glucopyranoside (compound 1) exhibited the best protective effects with no toxicity observed. Next, we showed compound 1 concentration-dependently inhibited necrotic cell death pathway activation and 2.5 mM compound 1 also prevented the loss of mitochondrial membrane potential, adenosine triphosphate production, and reactive oxygen species generation in mouse pancreatic acinar cells. Finally, we showed compound 1 protected against three clinically representative murine models of AP and significantly improved pancreatitis-associated acute lung injury. These data provide in vitro and in vivo evidence that one compound of diosgenyl saponins can be potential treatment for AP. This study suggests natural saponins may serve as fruitful sources for exploring/identifying potential therapies for inflammatory diseases.
Critical scaling of icosahedral medium-range order in CuZr metallic glass-forming liquids
Authors: Wu Z. W. , Li F. X. , Huo C. W. , Li M. Z. , Wang W. H. , Liu K. X.
Published: October 2016

The temperature evolution of icosahedral medium-range order formed by interpenetrating icosahedra in CuZr metallic glassforming liquids was investigated via molecular dynamics simulations. Scaling analysis based on percolation theory was employed, and it is found that the size distribution of clusters formed by the central atoms of icosahedra at various temperatures follows a very good scaling law with the cluster number density scaled by S−τ and the cluster size S scaled by |1 − Tc/T|−1/σ, respectively. Here Tc is scaling crossover-temperature. τ and σ are scaling exponents. The critical scaling behaviour suggests that there would be a structural phase transition manifested by percolation of locally favoured structures underlying the glass transition, if the liquid could be cooled slowly enough but without crystallization intervening. Furthermore, it is revealed that when icosahedral short-range order (ISRO) extends to medium-range length scale by connection, the atomic configurations of ISROs will be optimized from distorted ones towards more regular ones gradually, which significantly lowers the energies of ISROs and introduces geometric frustration simultaneously. Both factors make key impacts on the drastic dynamic slow-down of supercooled liquids. Our findings provide direct structure-property relationship for understanding the nature of glass transition.
Advanced Raman Spectroscopy of Methylammonium Lead Iodide: Development of a Non-destructive Characterisation Methodology
Authors: Pistor Paul , Ruiz Alejandro , Cabot Andreu , Izquierdo-Roca Victor
Published: October 2016

In recent years, there has been an impressively fast technological progress in the development of highly efficient lead halide perovskite solar cells. However, the stability of perovskite films and respective solar cells is still an open point of concern and calls for advanced characterization methods. In this work, we identify appropriate measurement conditions for a meaningful analysis of spin-coated absorber-grade perovskite thin films based on methylammonium (MA) lead iodide (MAPbI3) by Raman spectroscopy. The material under investigation and its derivates is the most commonly used for high efficiency devices in the literatures and has yielded working solar cell devices with efficiencies around 10% in our laboratory. We report highly detailed Raman spectra obtained with excitation at 532 nm and 633 nm and their deconvolution taking advantage of the simultaneous fitting of spectra obtained with varying excitation wavelengths. Finally, we propose a fast and contactless methodology based on Raman to probe composition variations and/or degradation of these perovskite thin films and discuss the potential of the presented technique as quality control and degradation monitoring tool in other organic-inorganic perovskite materials and complete solar cell devices.
Network Consistency Projection for Human miRNA-Disease Associations Inference
Authors: Gu Changlong , Liao Bo , Li Xiaoying , Li Keqin
Published: October 2016

Prediction and confirmation of the presence of disease-related miRNAs is beneficial to understand disease mechanisms at the miRNA level. However, the use of experimental verification to identify disease-related miRNAs is expensive and time-consuming. Effective computational approaches used to predict miRNA-disease associations are highly specific. In this study, we develop the Network Consistency Projection for miRNA-Disease Associations (NCPMDA) method to reveal the potential associations between miRNAs and diseases. NCPMDA is a non-parametric universal network-based method that can simultaneously predict miRNA-disease associations in all diseases but does not require negative samples. NCPMDA can also confirm the presence of miRNAs in isolated diseases (diseases without any known miRNA association). Leave-one-out cross validation and case studies have shown that the predictive performance of NCPMDA is superior over that of previous method.
Linearized texture of three-dimensional extracellular matrix is mandatory for bladder cancer cell invasion
Authors: Alfano Massimo , Nebuloni Manuela , Allevi Raffaele , Zerbi Pietro , Longhi Erika , Lucianò Roberta , Locatelli Irene , Pecoraro Angela , Indrieri Marco , Speziali Chantal , Doglioni Claudio , Milani Paolo , Montorsi Francesco , Salonia Andrea
Published: October 2016

In the fields of biomaterials and tissue engineering simulating the native microenvironment is of utmost importance. As a major component of the microenvironment, the extracellular matrix (ECM) contributes to tissue homeostasis, whereas modifications of native features are associated with pathological conditions. Furthermore, three-dimensional (3D) geometry is an important feature of synthetic scaffolds favoring cell stemness, maintenance and differentiation. We analyzed the 3D structure, geometrical measurements and anisotropy of the ECM isolated from (i) human bladder mucosa (basal lamina and lamina propria) and muscularis propria; and, (ii) bladder carcinoma (BC). Next, binding and invasion of bladder metastatic cell line was observed on synthetic scaffold recapitulating anisotropy of tumoral ECM, but not on scaffold with disorganized texture typical of non-neoplastic lamina propria. This study provided information regarding the ultrastructure and geometry of healthy human bladder and BC ECMs. Likewise, using synthetic scaffolds we identified linearization of the texture as a mandatory feature for BC cell invasion. Integrating microstructure and geometry with biochemical and mechanical factors could support the development of an innovative synthetic bladder substitute or a tumoral scaffold predictive of chemotherapy outcomes.
S100A8 promotes migration and infiltration of inflammatory cells in acute anterior uveitis
Authors: Wang Yuqin , Zhang Zuhui , Zhang Laihe , Li Xinxin , Lu Rui , Xu Peipei , Zhang Xuhong , Dai Mali , Dai Xiaodan , Qu Jia , Lu Fan , Chi Zailong
Published: October 2016

Uveitis, the pathologic condition of inflammation of the uvea, frequently leads to severe vision loss and blindness. S100A8 is a calcium-binding protein which mainly expresses in granulocytes and monocytes and plays a prominent role in the regulation of inflammatory processes and immune response. Here, we determined the role of S100A8-positive cells in acute anterior uveitis (AAU) and keratitis. In rat models of endotoxin (lipopolisaccharide, LPS) -induced uveitis (EIU) and keratitis, S100A8-positive granulocytes and monocytes increased significantly in the iris-ciliary body and cornea as well as in the blood. Interestingly, Glucocorticoids slightly increased S100A8 levels in leukocytes, but reduced its presence significantly in the iris-ciliary body after LPS injection. Moreover, inhibition of NF-kB activation remarkably suppressed both progression of AAU and total S100A8 levels in leukocytes and the iris-ciliary body after LPS administration. Additionally, S100A8 protein level was also found to be elevated in the serum of AAU patients parallel with the progression of AAU through the designated clinical stages. Thus, S100A8 plays a pivotal role in the processes of AAU through involvement in migration and infiltration of S100A8-positive cells. Our findings suggest that serum levels of S100A8 protein can be used to monitor inflammatory activity in AAU.
SNP-based heritability estimates of the personality dimensions and polygenic prediction of both neuroticism and major depression: findings from CONVERGE
Authors: Docherty A R , Moscati A , Peterson R , Edwards A C , Adkins D E , Bacanu S A , Bigdeli T B , Webb B T , Flint J , Kendler K S
Published: October 2016

Biometrical genetic studies suggest that the personality dimensions, including neuroticism, are moderately heritable (~0.4 to 0.6). Quantitative analyses that aggregate the effects of many common variants have recently further informed genetic research on European samples. However, there has been limited research to date on non-European populations. This study examined the personality dimensions in a large sample of Han Chinese descent (N=10 064) from the China, Oxford, and VCU Experimental Research on Genetic Epidemiology study, aimed at identifying genetic risk factors for recurrent major depression among a rigorously ascertained cohort. Heritability of neuroticism as measured by the Eysenck Personality Questionnaire (EPQ) was estimated to be low but statistically significant at 10% (s.e.=0.03, P=0.0001). In addition to EPQ, neuroticism based on a three-factor model, data for the Big Five (BF) personality dimensions (neuroticism, openness, conscientiousness, extraversion and agreeableness) measured by the Big Five Inventory were available for controls (n=5596). Heritability estimates of the BF were not statistically significant despite high power (>0.85) to detect heritabilities of 0.10. Polygenic risk scores constructed by best linear unbiased prediction weights applied to split-half samples failed to significantly predict any of the personality traits, but polygenic risk for neuroticism, calculated with LDpred and based on predictive variants previously identified from European populations (N=171 911), significantly predicted major depressive disorder case–control status (P=0.0004) after false discovery rate correction. The scores also significantly predicted EPQ neuroticism (P=6.3 × 10−6). Factor analytic results of the measures indicated that any differences in heritabilities across samples may be due to genetic variation or variation in haplotype structure between samples, rather than measurement non-invariance. Findings demonstrate that neuroticism can be significantly predicted across ancestry, and highlight the importance of studying polygenic contributions to personality in non-European populations.
Organocatalytic asymmetric formal arylation of benzofuran-2(3H)-ones with cooperative visible light photocatalysis
Authors: Liu Yang , Li Jiangtao , Ye Xinyi , Zhao Xiaowei , Jiang Zhiyong
Published: October 2016

An asymmetric arylation of benzofuran-2(3H)-ones with 1-naphthols has been developed through a cooperative visible light photocatalysis with asymmetric hydrogen-bond catalysis.
Duplications of an iron–sulphur tripeptide leads to the formation of a protoferredoxin
Authors: Scintilla Simone , Bonfio Claudia , Belmonte Luca , Forlin Michele , Rossetto Daniele , Li Jingwei , Cowan James A. , Galliani Angela , Arnesano Fabio , Assfalg Michael , Mansy Sheref S.
Published: October 2016

Based on UV-Vis, NMR, and EPR spectroscopies and DFT and molecular dynamics calculations, a model prebiotic [2Fe–2S] tripeptide was shown to accept and donate electrons.
Impact and importance of electrostatic potential calculations for predicting structural patterns of hydrogen and halogen bonding
Authors: Perera M. D. , Desper J. , Sinha A. S. , Aakeröy C. B.
Published: October 2016

Calculated molecular electrostatic potential difference (ΔMEP) of acceptor atoms in a multi component system will lead to different supramolecular architectures.
Ethynyl hydrogen bonds and iodoethynyl halogen bonds: a case of synthon mimicry
Authors: Aakeröy Christer B. , Welideniya Dhanushi , Desper John
Published: October 2016

The common electrostatic features of ethynyl and iodoethynyl hydrogen- and halogen-bond donors, respectively, lead to synthon mimicry which can be employed in synthetic crystal engineering for the construction of identical supramolecular assemblies in the solid-state.
Spin dynamics in HeH2+ molecular ion in intense laser fields
Authors: Korani Youssef , Sabzyan Hassan
Published: October 2016

A theoretical study is carried out on the effect of non-dipole interactions on the electron spin dynamics in the asymmetric diatomic HeH2+ in its first excited state in intense linearly polarized laser fields.
High color rendering index warm white light emitting diodes fabricated from AgInS2/ZnS quantum dot/PVA flexible hybrid films
Authors: Wang Lan , Kang Xiaojiao , Pan Daocheng
Published: October 2016

A high color rendering warm-white LED is fabricated by fixing suitable thicknesses of the green and red AIS/ZnS QD/PVA luminescent films on top of a conventional blue InGaN chip.
Poly(3,4-dioxythiophene) soft nano-network with a compatible ion transporting channel for improved electrochromic performance
Authors: Qin Leiqiang , Ding Zhiqiu , Hanif Muddasir , Jiang Jianxia , Liu Linlin , Mo Yueqi , Xie Zengqi , Ma Yuguang
Published: October 2016

A novel nano-soft network of poly(3,4-dioxythiophene) dimer (PM-BTE) was electropolymerized, which greatly promoted electrochromic performance compared with that of PEDOT by a compatible ion transporting channel.
Nanoparticles highly loaded with gentamicin sulfate by a combination of polyhydroxylated macromonomers and ROMP for the synthesis of bioactive biomaterials
Authors: Pichavant L. , Carrié H. , Durrieu M. C. , Héroguez V.
Published: October 2016

Nanoparticles highly loaded with gentamicin sulfate were synthesized by ring-opening metathesis copolymerization in a dispersion of norbornene with modified polyhydroxylated macromonomers.
Electrochemically synthesized sulfur-doped graphene as a superior metal-free cathodic catalyst for oxygen reduction reaction in microbial fuel cells
Authors: Han Thi Hiep , Parveen Nazish , Ansari Sajid Ali , Shim Jun Ho , Nguyen Anh Thi Nguyet , Cho Moo Hwan
Published: October 2016

Electrochemically synthesized S-GN was proved to be an efficient alternative cathode catalyst to Pt/C in microbial fuel cell.
An FT-IR spectroscopic study of the role of hydrogen bonding in the formation of liquid crystallinity for mixtures containing bipyridines and 4-pentoxybenzoic acid
Authors: Martinez-Felipe Alfonso , Cook Andrew G. , Abberley Jordan P. , Walker Rebecca , Storey John M. D. , Imrie Corrie T.
Published: October 2016

The relationships between liquid crystallinity and hydrogen bonding are studied in mixtures containing 4-pentoxybenzoic acid, 5OBA, and five bipyridines, XBiPy, with spacers having different flexibilities, by Fourier transform infrared spectroscopy.
Novel synthesis of an iron oxalate capped iron oxide nanomaterial: a unique soil conditioner and slow release eco-friendly source of iron sustenance in plants
Authors: Das Pallabi , Sarmah Kasturi , Hussain Nazneen , Pratihar Sanjay , Das Subhasish , Bhattacharyya Pradip , Patil Supriya A. , Kim Hak-Sung , Khazi Mohammed Iqbal A. , Bhattacharya Satya Sundar
Published: October 2016

Iron (Fe) is a vital plant-derived micronutrient in the human diet.
Antifungal ouzo nanoparticles from guar gum propionate
Authors: Kundu Sonia , Abdullah Md. Farooque , Das Aatrayee , Basu Aalok , Halder Asim , Das Mousumi , Samanta Amalesh , Mukherjee Arup
Published: October 2016

Synthesis of high DS guar gum propionate esters and the formation of self-assembly nanoparticles for fungus contact killing.
Constructing H+-triggered bubble generating nano-drug delivery systems using bicarbonate and carbonate
Authors: Wen Zuhuang , Long Yijuan , Yang Lili , Hu Jiangang , Huang Ning , Cheng Yuan , Zhao Li , Zheng Huzhi
Published: October 2016

Schematic illustration of the formation of bubble-generating nanosystems and killing of cancer cells by the quick release of CO2 bubbles.
3D reticulate CoxNi3−xS2 nanostructure on nickel foam as a new type of electroactive material for high-performance supercapacitors
Authors: Zhang Enping , Ni Yonghong
Published: October 2016

3D reticulate CoxNi3−xS2 nanostructures were grown on nickel foam using a simple precursor sulfuration route.
Synthesis of small diameter silver nanowires via a magnetic-ionic-liquid-assisted polyol process
Authors: Jo Hyun-Ah , Jang Hae-Won , Hwang Bu-Yeon , Kim Yong-Hoe , Kim Jin-Yeol
Published: October 2016

Silver nanowires (Ag NWs) with a small diameter of 20 nm and aspect ratio of 1000 were synthesized using the polyol method in the presence of a magnetic ionic liquid: 1-butyl-3-methylimidazolium tetrachloroferrate.
An improved model for the surface potential and drain current in negative capacitance field effect transistors
Authors: Xiao Y. G. , Ma D. B. , Wang J. , Li G. , Yan S. A. , Zhang W. L. , Li Z. , Tang M. H.
Published: October 2016

An improved model for the surface potential and drain current in negative capacitance ferroelectric field effect transistors (NC-FeFETs) was presented by introducing the doping concentration.
Heat transfer enhancement of water-based highly crumpled few-layer graphene nanofluids
Authors: Amiri Ahmad , Ahmadi Goodarz , Shanbedi Mehdi , Etemadi Mohammadmahdi , Mohd Zubir Mohd Nashrul , Chew B. T. , Kazi S. N.
Published: October 2016

Backward-facing step heat transfer of transitional and turbulent flows investigates in the presence of water-based HCFLG nanofluids.
Hydrosoluble aliphatic tertiary amine-containing hyperbranched polysiloxanes with bright blue photoluminescence
Authors: Niu Song , Yan Hongxia , Chen Zhengyan , Du Yuqun , Huang Wei , Bai Lihua , Lv Qing
Published: October 2016

Two hydrosoluble aliphatic tertiary amine-containing hyperbranched polysiloxanes are explored. The resulting conventional chromophore-free polymers, without any treatment, such as acidification or oxidation, can emit bright blue fluorescence.
Biodegradable poly(butylene succinate-co-terephthalate) nanofibrous membranes functionalized with cyclodextrin polymer for effective methylene blue adsorption
Authors: Wei Zhenzhen , Liu Yinli , Hu Hongmei , Yu Jianyong , Li Faxue
Published: October 2016

PBST and PBST/CDP nanofibrous membranes were prepared for the first time. PBST/CDP membranes were fabricated by means ofin situpolymerization. The morphologies of membranes were dependent on the CDP content. PBST/CDP membranes exhibited excellent adsorption capacity.
A recyclable and water soluble copper(i)-catalyst: one-pot synthesis of 1,4-disubstituted 1,2,3-triazoles and their biological evaluation
Authors: Nekkanti Shalini , Veeramani Karuna , Sujana Kumari S. , Tokala Ramya , Shankaraiah Nagula
Published: October 2016

A protocol for the synthesis of 1,4-disubstituted 1,2,3-triazoles via three-component reaction by a water soluble copper(i) complex has been developed.
White light emission from quantum dot and a UV-visible emitting Pd-complex on its surface
Authors: Gogoi Madhulekha , Chattopadhyay Arun
Published: October 2016

Near white light emission (CIE 0.35, 0.29) has been achieved as a combination of intraligand transition, aggregate induced emission and dopant emission followed by surface complexation on Qdot surface.
Time-dependent evolution of the nitrogen configurations in N-doped graphene films
Authors: Matsoso Boitumelo J. , Ranganathan Kamalakannan , Mutuma Bridget K. , Lerotholi Tsenolo , Jones Glenn , Coville Neil J.
Published: October 2016

Large-area time-controlled N-doped graphene films were grown on a Cu foil using an ammonia-assisted atmospheric pressure chemical vapour deposition (APCVD) technique.
Structure advantage and peroxidase activity enhancement of deuterohemin-peptide–inorganic hybrid flowers
Authors: Zhao Zijian , Zhang Ji , Wang Mingyang , Wang Zhi , Wang Liping , Ma Li , Huang Xuri , Li Zhengqiang
Published: October 2016

Hybridizing deuterohemin-peptide (DhHP-6) with copper phosphate to form hybrid flowers was prepared for preventing DhHP-6 aggregation.
Microstructural morphologies of CTAB micelles modulated by aromatic acids
Authors: Padsala Shailesh , Dharaiya Nilesh , Sastry Nandhibatla V. , Aswal Vinod K. , Bahadur Pratap
Published: October 2016

pH and temperature-dependent aggregation behaviour of CTAB with solubilized aromatic acids.
TiO2 nanoparticles vs. TiO2 nanowires as support in hydrogen peroxide direct synthesis: the influence of N and Au doping
Authors: Gemo Nicola , Menegazzo Federica , Biasi Pierdomenico , Sarkar Anjana , Samikannu Ajaikumar , Raut Dilip G. , Kordás Krisztián , Rautio Anne-Riikka , Mohl Melinda , Boström Dan , Shchukarev Andrey , Mikkola Jyri-Pekka
Published: October 2016

Nitrogen doping is a new strategy to improve catalysts for H2O2 direct synthesis.
Cycloelatanene A and B: absolute configuration determination and structural revision by the crystalline sponge method
Authors: Lee Shoukou , Hoshino Manabu , Fujita Makoto , Urban Sylvia
Published: October 2016

The crystalline sponge method revealed the absolute configuration of cycloelatanene A and B.
Calix[4]resorcinarene-based branched macromolecules for all-optical photorefractive applications
Authors: Liu Wei , Yang Haitao , Wu Wenbo , Gao Hongyan , Xu Shidang , Guo Qing , Liu Yingliang , Xu Shengang , Cao Shaokui
Published: October 2016

A simple design strategy for all-optical photorefractive (AOPR) materials is proposed and a high photorefractive effect is achieved without any external electric field.
Getting into mitochondria
Authors: Miernyk Ján A.
Published: October 2016

The human mitochondrial glutamate dehydrogenase isoenzymes (hGDH1 and hGDH2) are abundant matrix-localized proteins encoded by nuclear genes. The proteins are synthesized in the cytoplasm, with an atypically long N-terminal mitochondrial targeting sequence (MTS). The results of secondary structure predictions suggest the presence of two α-helices within the N-terminal region of the MTS. Results from deletion analyses indicate that individual helices have limited ability to direct protein import and matrix localization, but that there is a synergistic interaction when both helices are present [Biochem. J. (2016) 473, 2813–2829]. Mutagenesis of the MTS cleavage sites blocked post-import removal of the presequences, but did not impede import. The authors propose that the high matrix levels of hGDH can be attributed to the unusual length and secondary structure of the MTS.
Disulphide bond restrains the C-terminal region of thermostable direct hemolysin during folding to promote oligomerization
Authors: Kundu Nidhi , Tichkule Swapnil , Pandit Shashi Bhushan , Chattopadhyay Kausik
Published: October 2016

Pore-forming toxins (PFTs) are typically produced as water-soluble monomers, which upon interacting with target cells assemble into transmembrane oligomeric pores. Vibrio parahaemolyticus thermostable direct hemolysin (TDH) is an atypical PFT that exists as a tetramer in solution, prior to membrane binding. The TDH structure highlights a core β-sandwich domain similar to those found in the eukaryotic actinoporin family of PFTs. However, the TDH structure harbors an extended C-terminal region (CTR) that is not documented in the actinoporins. This CTR remains tethered to the β-sandwich domain through an intra-molecular disulphide bond. Part of the CTR is positioned at the inter-protomer interface in the TDH tetramer. Here we show that the truncation, as well as mutation, of the CTR compromise tetrameric assembly, and the membrane-damaging activity of TDH. Our study also reveals that intra-protomer disulphide bond formation during the folding/assembly process of TDH restrains the CTR to mediate its participation in the formation of inter-protomer contact, thus facilitating TDH oligomerization. However, once tetramerization is achieved, disruption of the disulphide bond does not affect oligomeric assembly. Our study provides critical insights regarding the regulation of the oligomerization mechanism of TDH, which has not been previously documented in the PFT family.
Continuity From Prelinguistic Communication to Later Language Ability: A Follow-Up Study From Infancy to Early School Age
Authors: Määttä Sira , Laakso Marja-Leena , Ahonen Timo , Tolvanen Asko , Westerholm Jari , Aro Tuija
Published: October 2016

Purpose

This longitudinal study examined the development of prelinguistic skills and the continuity of communication and language from the prelinguistic stage to school age.

Method

Prelinguistic communication of 427 Finnish children was followed repeatedly from 6 to 18 months of age (
n
= 203–322 at ages 6, 9, 12, 15, and 18 months), and its associations with language ability at ages 2;0 (
n
= 104), 3;0 (
n
= 112), 4;7 (
n
= 253), 5;3 (
n
= 102), and 7;9 (
n
= 236) were examined using latent growth curve modeling.

Results

Prelinguistic development across several skills emerged as a rather stable intraindividual characteristic during the first 2 years of life. Continuity from prelinguistic development to later language ability was indicated. The common level and growth of prelinguistic skills were significant predictors of language ability between ages 2;0 (years;months) and 7;9; the percentage explained varied between 10.5% and 53.3%. A slow pace of development across multiple skills, in particular, led to weaker language skills.

Conclusions

The results support (a) the idea of a developmental continuum from prelinguistic to linguistic ability and (b) the dimensional view of language ability by indicating that individual variations in early communication skills show consistency that extends beyond the toddler years. Our results also advocate developmental surveillance of early communication by emphasizing the significance of growth in predicting language development.

Supplemental Material

https://doi.org/10.23641/asha.14963937
Heterogeneous domain adaptation method for video annotation
Authors: Wang Han , Wu Xinxiao , Jia Yunde
Published: October 2016

In this study, the authors study the video annotation problem over heterogeneous domains, in which data from the image source domain and the video target domain is represented by heterogeneous features with different dimensions and physical meanings. A novel feature learning method, called heterogeneous discriminative analysis of canonical correlation (HDCC), is proposed to discover a common feature subspace in which heterogeneous features can be compared. The HDCC utilises discriminative information from the source domain as well as topology information from the target domain to learn two different projection matrices. By using these two matrices, heterogeneous data can be projected onto a common subspace and different features can be compared. They additionally design a group weighting learning framework for multi‐domain adaptation to effectively leverage knowledge learned from the source domain. Under this framework, source domain images are organised in groups according to their semantic meanings, and different weights are assigned to these groups according to their relevancies to the target domain videos. Extensive experiments on the Columbia Consumer Video and Kodak datasets demonstrate the effectiveness of their HDCC and group weighting methods.
Analytical modelling and prototyping a slotless surface‐inset PM machine
Authors: Boroujeni Samad Taghipour , Naghneh Hossein Bagheri
Published: October 2016

An analytical model is proposed for the prediction of the no‐load air gap magnetic flux density and the armature reaction in the slotless surface‐inset PM machines. For this purpose, the exact 2D solution of the Poisson equation is derived. In the modelling process, the rotor salient poles are taken away and some surface magnetisation currents are considered at the borders of the removed salient poles. The contribution of this work is finding the value of the surface magnetisation currents such that the rotor saliency is accurately considered. The field solution in the provided surface‐mounted PM machine is simply obtained by the separation of variables method. The machine back‐EMF and its inductances is obtained by the predicted flux density due to the PMs and the stator currents, respectively. In addition, using the resultant air gap flux density in the Maxwell stress tensor, the developed electromagnetic torque is computed. Finally, the ability of the proposed model is evaluated by finite element analysis as well as experimental tests.
Dipole modes with depressed amplitudes in red giants are mixed modes
Authors: Mosser B. , Belkacem K. , Pinçon C. , Takata M. , Vrard M. , Barban C. , Goupil M.-J. , Kallinger T. , Samadi R.
Published: October 2016

Context. Seismic observations with the space-borne Kepler mission have shown that a number of evolved stars exhibit low-amplitude dipole modes, which is referred to as depressed modes. Recently, these low amplitudes have been attributed to the presence of a strong magnetic field in the stellar core of those stars. Subsequently, and based on this scenario, the prevalence of high magnetic fields in evolved stars has been inferred. It should be noted, however, that this conclusion remains indirect.

Aims. We intend to study the properties of mode depression in evolved stars, which is a necessary condition before reaching conclusions about the physical nature of the mechanism responsible for the reduction of the dipole mode amplitudes.

Methods. We perform a thorough characterization of the global seismic parameters of depressed dipole modes and show that these modes have a mixed character. The observation of stars showing dipole mixed modes that are depressed is especially useful for deriving model-independent conclusions on the dipole mode damping. We use a simple model to explain how mode visibilities are connected to the extra damping seen in depressed modes.

Results. Observations prove that depressed dipole modes in red giants are not pure pressure modes but mixed modes. This result, observed in more than 90% of the bright stars (mV ≤ 11), invalidates the hypothesis that depressed dipole modes result from the suppression of the oscillation in the radiative core of the stars. Observations also show that, except for visibility, seismic properties of the stars with depressed modes are equivalent to those of normal stars. The measurement of the extra damping that is responsible for the reduction of mode amplitudes, without any prior on its physical nature, potentially provides an efficient tool for elucidating the mechanism responsible for the mode depression.

Conclusions. The mixed nature of the depressed modes in red giants and their unperturbed global seismic parameters carry strong constraints on the physical mechanism responsible for the damping of the oscillation in the core. This mechanism is able to damp the oscillation in the core but cannot fully suppress it. Moreover, it cannot modify the radiative cavity probed by the gravity component of the mixed modes. The recent mechanism involving high magnetic fields proposed for explaining depressed modes is not compliant with the observations and cannot be used to infer the strength and prevalence of high magnetic fields in red giants.
Endovascular Neurosurgery in the Northern Macro- region of Rio Grande do Sul: Part I
Authors: Vanzin José , Azambuja Jr Nério , Frighetto Leonardo , Frohlich Alan , Varela Daniel , Mesquita Filho Paulo , Roman Alex , Manzato Luciano
Published: October 2016

Introduction The city of Passo Fundo, in the north of the Rio Grande do Sul state, has been standing out in the health care field for many years. The state has become a reference in endovascular interventional neuroradiology. We will cover 10 years of experience in this area and divide our observations in 3 parts: cerebral angiograms (part I), carotid angioplasties (part II) and intracranial aneurysms (part III). The goal of part I is to statistically assess the cerebral angiograms, their indications, risks and complications, as well as to do a technical review.

Materials and Methods A retrospective study from 2005 to 2015 with a total of 5,567 interventional neuroradiology procedures performed. A total of 4,114 angiograms, 639 embolizations of intracranial aneurysms, 414 carotid angioplasties, 143 embolizations of cerebral arteriovenous malformations, 32 embolizations of dural arteriovenous fistulas, 102 cerebral vasospasm treatments, 21 treatments of epistaxis, 36 embolizations of craniocervical tumor, 25 thrombolysis of ischemic stroke, 18 vertebroplasties and 13 embolizations of arteriovenous malformations of the face.

Results A total of 4,084 procedures performed, 21,811 vessels studied, average vase 7.62/2.82 vessel and patient/procedure. Of these, 2,536 were diagnostic procedures and 1,548 angiographic controls. Of the total, 1,188 patients received only an angiogram, 27.14% of which were therapeutic procedures. We obtained a total of 3.89% complications: 2.33% reflection vasovagal, 0.56% allergic skin reaction, anaphylactic shock 0.07%, 0.27% femoral hematoma, 0.26% transient neurological deficit, 0.12% permanent neurological deficit and no case of death.

Conclusion Cerebral angiography in adults, children and infants is a safe procedure with low risk of permanent neurological complications.
Factors Associated with Knee Stiffness following Surgical Management of Multiligament Knee Injuries
Authors: Westermann Robert , Cook Shane , Glass Natalie , Amendola Ned , Wolf Brian , Bollier Matthew , Hanley Jessica
Published: October 2016

Postoperative knee stiffness can influence outcomes following operative treatment of multiligament knee injuries (MLKIs). The purpose of this study was to evaluate patient and surgical factors that may potentially contribute to stiffness following surgery for MLKIs. All surgically managed MLKIs involving two or more ligaments over a 10-year period at a single level one trauma center were included in this study. A retrospective review was performed to gather objective data related to the development of knee stiffness after surgery. Patients were classified as “stiff” postoperatively if they (1) had a flexion contracture greater than 10 degrees, (2) failed to reach 120 degrees of flexion at final follow-up, or (3) underwent a manipulation under anesthesia with or without arthroscopic lysis of adhesions to improve range of motion. Patient and surgical factors were evaluated systematically to determine factors associated with stiffness. The mean age of the cohort was 27.6 years at the time of surgery and mean follow-up was 50 weeks. Overall, 26/121 (21.5%) knees were diagnosed with postoperative stiffness. In the acute postoperative phase, 17 patients underwent manipulation under anesthesia. There were no significant differences in age, body mass index, associated injuries, mechanism, external fixation use or surgical timing (acute vs. chronic) between stiff and normal knees. Factors associated with the development of postoperative stiffness included knee dislocation (p = 0.04) and surgical intervention on three or more ligaments (p = 0.04). Careful attention to postoperative rehabilitation regimens should be given to patients with knee dislocations and/or those undergoing reconstruction or repair of three or more injured ligaments. Surgeons may utilize spanning external fixation if necessary without increasing the rate of long-term stiffness. Further, acute surgery does not appear to influence rates of postoperative stiffness or the need for manipulation.
Zehn Jahre Qualitätsmanagement: Wahrnehmung und Stellenwert aus Sicht von Hausärzten in einem Ärztenetz
Authors: Kühlein T. , Madlo-Thiess F. , Wambach V. , Schaffer S.
Published: October 2016

Hintergrund: Vor 10 Jahren wurde Qualitätsmanagement (QM) für den ambulanten Sektor verpflichtend eingeführt.

Ziel der Arbeit: Wir wollten wissen, wie die Einführung von QM von Hausärzten erlebt wurde, welchen Stellenwert es heute für sie hat und wie sie die Zukunft von QM einschätzen.

Material und Methoden: In einem qualitativen Studiendesign wurden Ärzte eines Ärztenetzes in Experteninterviews anhand eines semistrukturierten Interviewleitfadens befragt. Die verschriftlichten Interviews wurden in einem zunächst induktiven, dann deduktiven Prozess in Triangulation kodiert. In einem Diskussions- und Einigungsprozess konnten Themenfelder und Kodefamilien identifiziert werden. Die Befragung endete nach Informationssättigung.

Ergebnisse: Für die Standardisierung einiger Basisprozesse wie Hygiene bestand Einverständnis. QM ließe sich jedoch kaum auf einen Bereich übertragen, der wesentlich von zwischenmenschlicher Beziehung und Kommunikation geprägt sei. Die Ärzte gaben an, QM auf ein erträgliches und für sie sinnvolles Maß reduziert zu haben. Zertifizierung wurde weiterestgehend abgelehnt. Die Erwartungen für die nächsten 10 Jahre waren im Sinne immer mehr bürokratischer Vorgaben eher pessimistisch.

Schlussfolgerung: Die gesetzliche Einführung von QM war ein Eingriff der Gesellschaft in den professionellen Bereich der Ärzte. Statt passivem Widerstand und Beschränkung von QM auf ein erträgliches Minimum könnte eine engagierte eigenständige Qualitätsarbeit helfen, gesellschaftliches Vertrauen zurückzugewinnen, um die notwendige professionelle Autonomie zu erhalten.
Ergebnisse strukturierter Fokusgruppenarbeit zur Professionalisierung des zahnärztlichen Gutachterwesens in Deutschland
Authors: Brauer H. , Walther W. , Dick M.
Published: October 2016

Hintergrund: Im Mittelpunkt der professionellen Selbstkontrolle steht in der Medizin das Gutachterwesen. Um im zahnärztlichen Gutachterwesen einen gezielten Entwicklungsimpuls zu setzen, wurde eine Reflexion aus der Perspektive der Gutachter initiiert.

Methodik: 5 Gruppendiskussionen zum Thema „Professionalisierung des zahnärztlichen Gutachterwesens“ wurden mit 32 erfahrenen Gutachtern als Diskutanten durchgeführt und mittels qualitativer Inhaltsanalyse ausgewertet.

Ergebnisse: Die Auswertung ergab einen Katalog mit 68 Ideen, welche in 15 Kategorien eingeteilt wurden. Diskutierte Lösungsansätze waren z. B. Gutachter-Qualitätszirkel, kollegialer/interprofessioneller Austausch, Supervision bei Neugutachtern und Feedback für Gutachter/Zahnärzte.

Schlussfolgerung: Mehr Reflexivität trägt zu einer Verbesserung des Gutachterwesens sowie zur Qualitätssicherung bei der Begutachtung bei.
Errata: Selbstmanagementförderung bei chronischer Krankheit für Menschen mit Migrationshintergrund – ein Gruppenkurs mit Peer-Ansatz als Beitrag zu gesundheitlicher Chancengerechtigkeit
Authors: Zanoni S. , Gabriel E. , Salis Gross C. , Deppeler M. , Haslbeck J.
Published: October 2016

Erratum

Zanoni S, Gabriel E, Salis Gross C, Deppeler M, Haslbeck J. Selbstmanagementförderung bei chronischer Krankheit für Menschen mit Migrationshintergrund – ein Gruppenkurs mit Peer-Ansatz als Beitrag zu gesundheitlicher Chancengerechtigkeit. Gesundheitswesen 2016;

Bei diesem Beitrag wurde der engl. Titel korrigiert. Korrekt ist: Chronic Disease Self-management Support for People with a Migrant Background:
towards a Peer-led Group Program to Improve Equity in Health