bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Novel Target DCTPP1 for Colorectal Cancer Therapy with the Natural Small-molecule Inhibitors Regulating Metabolic Reprogramming
Authors: Feng Li , Wang Xinjia , Guo Xinrui , Shi Liyuan , Su Shihuang , Li Xinjing , Wang Jia , Tan Ninghua , Ma Yi , Wang Zhe
Published: May 2024

Colorectal cancer (CRC) is one of the most common malignant tumors. Identification of new effective drug targets for CRC and exploration of bioactive small-molecules are clinically urgent. The human dCTP pyrophosphatase 1 (DCTPP1) is a newly identified pyrophosphatase regulating the cellular nucleotide pool but remains unexplored as potential target for CRC treatment. Here, twelve unprecedented chemical architectures terpene-nonadride heterodimers (1–12) and their monomers (13–20) were isolated from endophyte Bipolaris victoriae S27. Compounds 1–12 represented the first example of terpene-nonadride heterodimers, in which nonadride monomers of 1 and 2 were also first example of 5/6 bicyclic nonadrides. A series of assays showed that 2 could repress proliferation and induce cell cycle arrest, apoptotic and autophagic CRC cell death in vitro and in vivo. Clinical cancer samples data revealed that DCTPP1 was a novel target associated with poor survival in CRC. DCTPP1 was also identified as a new target protein of 2. Mechanistically, compound 2 bound to DCTPP1, inhibited its enzymatic activity, intervened with amino acid metabolic reprogramming, and exerted anti-CRC activity. Our study demonstrates that DCTPP1 was a novel potential biomarker and therapeutic target in CRC, and 2 was the first natural anti-CRC drug candidate targeting DCTPP1.
M2ara: unraveling metabolomic drug responses in whole-cell MALDI mass spectrometry bioassays
Authors: Enzlein Thomas , Geisel Alexander , Hopf Carsten , Schmidt Stefan
Published: May 2024

Fast computational evaluation and classification of concentration responses for hundreds of metabolites represented by their mass-to-charge (m/z) ratios is indispensable for unraveling complex metabolomic drug actions in label-free, whole-cell Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI MS) bioassays. In particular, the identification of novel pharmacodynamic biomarkers to determine target engagement, potency and potential polypharmacology of drug-like compounds in high-throughput applications requires robust data interpretation pipelines. Given the large number of mass features in cell-based MALDI MS bioassays, reliable identification of true biological response patterns and their differentiation from potentially present measurement artefacts is critical. To facilitate the exploration of metabolomic responses in complex MALDI MS datasets, we present a novel software tool, M2ara. Implemented as a user-friendly R-based shiny application, it enables rapid evaluation of Molecular High Content Screening (MHCS) assay data. Furthermore, we introduce the concept of Curve Response Score (CRS) and CRS fingerprints to enable rapid visual inspection and ranking of mass features. In addition, these CRS fingerprints allow direct comparison of cellular effects among different compounds. Beyond cellular assays, our computational framework can also be applied to MALDI MS-based (cell-free) biochemical assays in general.
Inner-bond-cleavage approach to figure-eight macrocycles from planar aromatic hydrocarbons
Authors: Yoshina Reiji , Hirano Junichiro , Nishimoto Emiko , Sakamoto Yuki , Tajima Keita , Minabe Shunsuke , Uyanik Muhammet , Ishihara Kazuaki , Ikai Tomoyuki , Yashima Eiji , Omine Takuya , Ishiwari Fumitaka , Saeki Akinori , Kim Jinseok , Oh Juwon , Kim Dongho , Liu Guanting , Yasuda Takuma , Shinokubo Hiroshi , Fukui Norihito
Published: May 2024

Contemporary synthetic strategies in organic chemistry rely heavily on the stepwise formation of bonds between fragments of the target compound (‘bottom-up’ approach). Figure-eight aromatic macrocycles represent a considerable challenge for the bottom-up approach, and the lack of versatile synthetic routes to such macrocycles prevents the use of their distinctive chiral D2-symmetric structures in advanced materials. Herein, we demonstrate the oxidative cleavage of internal double bonds of planar polycyclic aromatic hydrocarbons enables the scalable, catalytic, and enantioselective synthesis of figure-eight macrocycles. These macrocycles, with incorporated carbonyl groups, serve as a chiral electron-acceptor unit to furnish a thermally activated delayed fluorescence material with efficient circularly polarized luminescence. The inner-bond-cleavage approach is thus an effective alternative to the bottom-up approach, proving access to hitherto inaccessible materials.
Feature attributions for water-solubility predictions obtained by artificial intelligence methods and chemists
Authors: Sadakane Teruhisa , Nakata Koki , Suda Kayo , Yokogawa Daisuke
Published: May 2024

Recently, the field of explainable artificial intelligence has attracted significant research interest, with a particular focus on “feature attribution” in the field of chemistry. However, studies comparing the relationship between artificial-intelligence- and human-based feature attributions when predicting the same outcome are scarce. Hence, the current study aims to investigate this relationship by comparing machine-learning-based feature attributions (graph neural networks and integrated gradients) with those of chemists (Hansch–Fujita method) when predicting water solubility. The findings reveal that the artificial-intelligence-based attributions are similar to those of chemists despite their distinct origins.
The central role of oxo clusters in zirconium- and hafnium-based esterification catalysis.
Authors: Pulparayil Mathew Jikson , Seno Carlotta , Jaiswal Mohit , Simms Charlotte , Reichholf Nico , Van den Eynden Dietger , Parac-Vogt Tatjana N. , De Roo Jonathan
Published: May 2024

Oxo clusters are a unique link between oxide nanocrystals and MOFs, representing the limit of downscaling each of the respective crystals. Here, we show the superior catalytic activity of Zr12O8(OH)8(OOCR)24 clusters, compared to zirconium MOF UiO-66 and ZrO2 nanocrystals. We focus on esterification reactions given their general importance in consumer products and the challenge of converting large substrates. Oxo clusters have a higher surface-to-volume ratio than nanocrystals, rendering them more active. For large substrates, e.g., oleic acid, MOF UiO-66 has negligible catalytic activity while clusters provide almost quantitative conversion, a fact we ascribe to limited diffusion of large substrates through the MOF pores. Cluster do not suffer from limited mass transfer and we also obtain high conversion in solvent-free reactions with sterically hindered alcohols (hexanol, 2-ethylhexanol, benzyl alcohol and neopentyl alcohol). The cluster catalyst can be recovered and shows identical activity. The structural integrity of the cluster is confirmed using X-ray total scattering and Pair Distribution Function analysis. Even more, when homogeneous zirconium (or hafnium) alkoxides are used as catalyst, the same oxo cluster is retrieved, showing that oxo clusters are the active catalytic species, even in previously assumed homogeneously catalyzed reactions.
What is preventing individual climate action? Impact awareness and perceived difficulties in changing transport and food behaviour
Authors: Timmons Shane , Andersson Ylva , Lee Maria , Lunn Pete
Published: May 2024

Mitigating climate change requires large and, by historical standards, rapid changes to policy, business processes and individual behaviour. This report examines awareness of and perceived difficulty with individual behaviour change with respect to two actions associated with high levels of emissions: transport and food choice. A nationally representative sample of 1,200 adults completed an online study about everyday transport and food behaviour, run in September 2023. After completing a diary task about the previous day, participants identified the parts of their day that mattered most for their carbon footprint. Answers were elicited via incentivised, open text questions to prevent pre-set options biasing responses. Participants also answered standard survey questions about their use of various modes of transport in a typical week and their consumption of various foods. Participants who reported wanting to change their transport behaviour and diet ‘to reduce their carbon footprint’ (47 per cent and 42 per cent, respectively) listed reasons why it is difficult for them to do so, again via open text questions.
Big Data no Turismo: conceitos e aplicações
Authors: Ruas Rayane
Published: May 2024

Rayane Ruas explica de forma clara que “o Big Data está relacionado com o ato de gerar, capturar e processar uma grande quantidade de dados”, e explica que esta é uma ferramenta essencial para a revolução do processo de tomada de decisão em turismo. De fato, o Big Data aproveita grandes volumes de dados que, em muitos casos, estão disponíveis na internet e são de fácil acesso, colocando-se como alternativa a outras formas de coleta de dados (por exemplo, formulários de pesquisa) bastante mais onerosas e demoradas. Um dos caminhos para inovar – bem como para antecipar tendências, compreender o mercado e, consequentemente, ter ganhos de rentabilidade –, é precisamente aproveitar a capacidade que nos dá o contexto digital para coletar dados com um esforço mínimo, para informar as decisões dos gestores. Como explica Rayane Ruas com rigor e detalhe, em uma linguagem simples e acessível, o Big Data é uma matéria-prima essencial para a decisão dos gestores turísticos. Permite compreender tanto a jornada do turista como a eficácia das estratégias dos concorrentes. Permite tomar decisões informadas e precisas, criando claras vantagens competitivas em relação às empresas que baseiam o seu marketing e as suas estratégias em formas convencionais de obtenção de dados. Apesar de a literatura sobre Big Data estar finalmente a crescer, faltava ainda um livro que desmistificasse o conceito e guiasse a sua aplicação no setor do turismo. Especialmente, uma obra com exemplos inspiradores e uma clara identificação dos requisitos para uma implementação bemsucedida. Este livro é, assim, uma ferramenta para gestores do setor do turismo com curiosidade sobre o tema e que pretendam trabalhar Big Data, não sabendo por onde começar. Em um meio digital pleno de oportunidades e desafios, e em que a relevância da comunicação e do marketing digital fazem toda a diferença para ser encontrado e ser ouvido, o Big Data permite, em última análise, identificar de forma rigorosa quem são os nossos clientes ideais e definir estratégias eficazes para os alcançar, encantar e envolver. E para ganhar vantagens competitivas, rentabilidade e diferenciação. É, por isso, uma ferramenta urgente para se compreender e ser adotada no caminho da transformação digital.
Adaptive Workload Scheduling Design in Parallel Computational Framework
Authors:   Assistant Professor, Department of CSE East Point College of Engineering and technology Bengaluru, Karnataka, INDIA, Bakka Jagadevi , Lingareddy Sanjeev ,   Principal Vijaya Vittala Institute of Technology, Bangalore
Published: May 2024

In the recent years, many researchers have showed a great deal of interest to improve the scheduling of workload in the cloud platforms. On the other hand, to carry out the execution of the scientific workloads in the cloud environment, it consumes much time and is expensive; hence, it is neither time efficient nor cost-efficient. Due to this reason, many research studies have been carried, by which the researchers tend to reduce the processing time and make a cost-efficient method as the users are charged based on the usage. Very few studies have been done to optimize the cost with processing time and energy parameters together in order to meet the Service Level Agreement (SLA) and Quality of Service (QoS) of the workload task. Hence, in this paper, we present an Adaptive Workload Scheduling (AWS) model that ensures the Task Level SLA prerequisites in a heterogeneous distributed-computing environment. This AWS- model approach reduces the amount of energy and time needed to execute a given workloads. Cybershake scientific workload has been utilized for the studying proposed AWS model. When model was compared with the standard workload scheduling approach, our model reduced the consumption of cost, energy and time.
Generative Adversarial Networks for Malware Detection in Cloud Computing Environments
Authors:   Assistant Professor, Department of CSE East Point College of Engineering and Technology Bangalore, INDA, L Shammi , Kousar Dr.Heena ,   Associate Professor, Department of CSE East Point College of Engineering and Technology Bangalore, INDA
Published: May 2024

In recent years, the proliferation of sophisticated malware threats has necessitated the development of advanced detection techniques to safeguard computer systems and networks. Generative Adversarial Networks (GANs), a class of deep learning models comprising a generator and a discriminator trained in an adversarial manner, have emerged as a promising tool for improving malware detection capabilities. Traditional signature-based malware detection struggles to keep pace with the ever-evolving threat landscape in cloud computing environments. This paper proposes a novel approach utilizing Generative Adversarial Networks (GANs) for enhanced malware detection. The GAN architecture generates realistic malware samples, refining the discriminator's ability to differentiate between legitimate and malicious software. This fosters a model adept at identifying zero-day attacks and unseen malware variants. Evaluation in a cloud environment assesses detection accuracy, efficiency, and generalizability. Findings underscore GANs' potential to enhance cloud-based malware detection, securing the future of cloud computing.
Development of an Android Application for Smart Parking System
Authors:   Associate Professor, Dept. of CSE East Point College of Engineering and Technology, Bangalore, INDIA, Gopal Neha
Published: May 2024

Now-a-days, vehicle parking has become a major problem in urban areas with the shortage of parking spaces. It is very difficult and frustrating to find a parking space in most metropolitan areas, especially during the rush hours to solve this problem. The project entitled smart parking system using android application, the major motivation of this project is to reduce the traffic congestion in roads, multi storied buildings and malls due to unavailability of parking spaces. The proposed application provides an easy way for reservation of parking slot. In this application user can view various parking areas and also view whether space is available or not. The project displays the nearest empty slot if present with respect to user location. This system gives a further feature of cancelling the bookings. Users may even make price online primarily based totally at the time taken for the reserved area the quantity might be calculated and the person can make charge. This project provides a good insight into the guidance, monitoring and reservations components of the smart parking and directions to the future development.
Prediction of Air Quality Data with Feature Selection and Machine Learning Algorithms
Authors:   Assistant Professor, Department of CSE East Point College of Engineering and Technology,  Bangalore, INDIA, Nivedita Pandey
Published: May 2024

Predicting air quality is essential for managing public health and the environment. This study looks into how well machine learning (ML) algorithms forecast air quality data and incorporates feature selection strategies for better model performance. Air pollution is one of the biggest issues facing humanity today. Every year, air pollution causes millions of deaths due to direct or indirect effects. These days, there is a critical need for effective measures to mitigate the negative impacts of air pollution. Usually, the answers to the issues with air pollution are hasty decisions that don't end up helping. It is vital to concentrate efforts on the pollutants that cause the majority of air pollution in order to create an efficient counter-strategy. In many industrial and urban regions today, monitoring and safeguarding air quality has emerged as one of the government's top priorities. The combustion of fossil fuels, traffic and weather conditions, and industrial parameters
Enhancing Signature Forgery Detection System Using CNN-SVM
Authors:   Assistant Professor, Department of CSE East Point College of Engineering and Technology, Bangalore, INDIA, A Manimegalai
Published: May 2024

Handwritten signatures are widely used as a means of personal identification and authentication. Many documents like bank cheques and legal transactions require signature verification. But considering a large number of documents, it is a very difficult and time-consuming task. Therefore, ensuring the necessity for a robust automatic signature verification tool that aims to reduce fraud in all related financial transaction sectors. The current visual verification depends mainly on the experience, mood, and working environment of the verifier which ultimately wastes both time and money. Moreover, it is difficult for the eyes of any experts to precisely verify the ratios between lines and angles of a genuine signature to a fraud signature. Therefore, we propose an automatic signature verification technique using the recent advances in image processing and machine learning.
The Synergy of Artificial Intelligence and the Internet of Things: Transforming Connectivity and Intelligence
Authors:   Assistant Professor/ Department of CSE East Point College of Engineering and Technology,  Bangalore, INDIA, Kumar Pavan
Published: May 2024

Artificial Intelligence (AI) and the Internet of Things (IoT) represent two powerful technological forces that, when combined, have the potential to revolutionize numerous aspects of our daily lives. This research article explores the transformative impact of AI on the IoT domain, examining how AI techniques enhance connectivity, intelligence, and decision-making capabilities within IoT systems. Through a comprehensive analysis of current research, applications, and future trends, this article illuminates the synergistic relationship between AI and IoT, offering insights into its implications for various industries and society as a whole.
Historical Review and Theoretical Thinking on Platforms, Labor and Media Studies
Authors: 孙 萍 , 廖 文钰 , 陈 欣欣
Published: May 2024

半个世纪后的今天,传播学语境下的劳动议题不再是达拉斯·斯迈兹笔下的“盲点”,而是成为“热点”。但是当下以平台化劳动为主的研究成果虽然丰硕,却也存在框架单一和概念混乱等问题。本研究尝试从媒介发展史的角度探究劳动形态与媒介发展形态之间的关系,以此理解平台化劳动的特点并尝试突破当下劳动研究“控制—反抗”的二元理论框架。传播学语境下的劳动生产伴随着不同的媒介技术发展而呈现出不同的样态;与此同时,劳动类别随着媒介的发展呈现“叠加状态”,即前一媒介发展阶段的劳动样态会延续并以新的形式在当今媒介技术生态下继续存在。现阶段平台化劳动的具身性、数据化、关系性特征正是媒介与劳动历史互动而成的结果。传播学视野下的数字劳动研究需要更多地观照媒介技术发展的底层逻辑并增加历史和对比的视野。
Towards a Regionally Responsive Network implementation challenges in New Zealand’s reforms to vocational education
Authors: Brownie Sharon , Broman Patrick , Comer Leith , Cooney Catherine , De Wet Leon
Published: May 2024

New Zealand has been undertaking major reform of its vocational education sector since 2019. Changes have been extensive, including the establishment of a national delivery body (Te Pūkenga), and the formation of workforce development councils, regional skills leadership groups, centres of vocational excellence, and a coalition of expert Māori advisors (Te Taumata Aronui). While a new government is reconsidering these structures, including stating a commitment to disestablish Te Pūkenga, it is timely to explore the key policy issues facing the vocational education sector. Accordingly, this case study considers how best to balance local and national interests. We suggest changes to ensure that the sector is well structured, governed and funded, and meets the needs of the communities it serves.
Can We Have the Population Statistics We Need Without a Regular Census?
Authors: Cook Len
Published: May 2024

As the New Zealand government is progressing plans for substituting the census as we know it for information held in state agency records, the huge gap between what we need population statistics for, and the scope and quality of existing statistics stays under the radar. The scale and volatility of migration, the impact of increased longevity and the different demographic dynamics of fast-growing ethnic communities must be continually measured in ways we can trust. Confidence that the statistical qualities of the census would be replicated requires transparency, validation and independent peer review if trust is to be maintained.
Evaluation of the Clinical Advantages of Laparoscopic Transverse Abdominal Wall Suspension in the Treatment of Pelvic Organ Prolapse
Authors: Wang Chao , Feng Shuo
Published: May 2024

Objective: To evaluate the clinical advantages of laparoscopic transverse abdominal wall suspension in treating pelvic organ prolapse. Methods: Sixty patients diagnosed with moderate to severe pelvic organ prolapse and underwent surgical treatment in our hospital between January 2022 and December 2023 were selected. According to different surgical methods, they were divided into an observation group (given laparoscopic transverse abdominal wall suspension) and a control group (given transvaginal mesh implantation), with 30 subjects/group. The data on perioperative-related indicators, quality of life scores, postoperative recurrence, and complications of the two groups of patients were collected. Results: The postoperative hospitalization days and intraoperative bleeding volume of the observation group were significantly lower than those of the control group, but had longer operation time than that of the control group (P < 0.05). The differences between the two groups were statistically significant 6 months after surgery, and the Pelvic Floor Disease Quality of Life Impact Questionnaire (PFIQ-7) score of the observation group was significantly higher than the control group (P < 0.05). Both groups of patients completed 12 months of follow-up without any postoperative recurrence. The number of complications in the observation group was slightly lower than that of the control group (P > 0.05). Conclusion: Laparoscopic transverse abdominal wall suspension was more effective in treating pelvic organ prolapse and is an ideal surgical procedure.
Evaluation of the Effectiveness and Efficiency of Extracorporeal Shock Wave Combined with Rehabilitation Training in the Treatment of Muscle Articulation Chronic Pain
Authors: Han Dongchou , Feng Qiya , Fu Yingmei , Zhang Feijian , Chen Dazhen , Wu Junmei
Published: May 2024

Objective: To analyze the effect of combined extracorporeal shock wave and rehabilitation training treatment in patients with muscle articulation chronic pain (MACP). Methods: Ninety-seven MACP patients admitted to our hospital from September 2021 to September 2023 were randomly selected and were divided into Group A (control group, 46 cases, rehabilitation training treatment) and Group B (observation group, 51 cases, extracorporeal shock wave with rehabilitation training treatment), and outcomes of the two groups were compared. Results: The treatment efficiency, post-treatment clinical indexes (upper and lower limb function scores, activities of daily living (ADL) scores, visual analog scale (VAS) scores), and short-form 36 (SF-36) scores of Group B were better than those of Group A (P < 0.05). Conclusion: Combined extracorporeal shock wave and rehabilitation training treatment for MACP patients improved their limb function, daily activities, quality of life, and reduced pain.
Analysis of The Correlation Between inhA Gene Mutation and Resistance to Protionamide in Drug-Resistant Mycobacterium Tuberculosis
Authors: Huang Xulin , Zheng Tian , Li Shutao
Published: May 2024

Objective: To investigate the characteristics of katG and inhA gene mutations in multidrug-resistant tuberculosis (MDR-TB), pre-extensively drug-resistant tuberculosis (preXDR-TB), and their correlation with resistance to protionamide (Pto). Methods: A total of 229 patients with MDR-TB and pre-XDR-TB diagnosed in the Eighth Affiliated Hospital of Xinjiang Medical University from January 2020 to February 2024 were selected to analyze the characteristics of katG and inhA mutations in MTB clinical isolates and their correlation with Pto resistance. Results: The mutation rate of katG (with or without inhA mutation) was 85.2%. The mutation rates in MDR-TB and pre-XDR-TB were 87.4% (125/143) and 81.4% (70/86), respectively. The mutation rate of inhA (including katG mutation) was 14.8% (34/229), which was 12.6% (18/143) and 18.6% (16/86) in MDR-TB and pre-XDR-MTB, respectively. There was no difference in mutation (P > 0.05). Conclusion: The total resistance rate to Pto in 229 strains was 8.7% (20/229), which was 8.4% (12/143) and 9.3% (8/86) in MDR-TB and pre-XDR-TB, respectively. Among the inhA mutant strains, 13 were resistant to the Pto phenotype, and the resistance rate was 65% (13/20). In MDR-TB and pre-XDR-TB strains resistant to Pto, inhA gene mutations occurred in 66.7% (6/9) and 63.6% (7/11), respectively. The resistance rates of MDR-MTB and pre-XDR-TB strains without inhA gene mutation to Pto were 2.4% (3/125) and 5.7% (4/70), respectively.
Observation on the Clinical Effect of Applying Vinaclat Combined with Demethylation Drug Therapy in Patients with Acute Myeloid Leukemia
Authors: Niu Ben , Hou Limin
Published: May 2024

Objective: To investigate the therapeutic effect of applying venetoclax combined with demethylating drugs in treating patients with acute myeloid leukemia (AML). Methods: Eighty cases of AML patients treated with venetoclax combined with demethylating drugs in our hospital were selected from March 2021 to March 2024, including 40 cases of primary treatment patients and 40 cases of relapsed and refractory patients. The efficacy and safety of the combined drug therapy was analyzed. Results: The primary treatment group was presented with a complete remission (CR) rate of 40.5%, partial remission (PR) rate of 47.50%, no response (NR) rate of 12.50%, and a remission rate of 87.50%. The relapsed- refractory group was presented with a CR rate of 37.50%, PR rate of 42.50%, NR rate of 17.50%, and a remission rate of 87.50%. There was no statistical significance between the groups (P > 0.05). The hematological adverse reactions of the combined treatment for AML were leukopenia and the non-hematological adverse reactions were mainly infections, with an incidence rate of 87.50%. Conclusion: The efficacy of venetoclax combined with demethylating drugs in AML was remarkable and the treatment regimen can be adjusted according to the treatment-resistant response.
Spatial analysis of fire sevetiry and its relationship with landscape configuration in savanna areas of Sempre Vivas National Park, Brazil
Authors: Salomão Natália Viveiros , Fernandes Geraldo Wilson , Gonzaga Anne Priscila Dias , Baronío Gudryan Jackson , Mucida Danielle Piuzana , Moraes Marcelino Santos , Silva Marcelo Dutra , Silva Leovandes Soares , Pereira Israel Marinho , Machado Evandro Luiz Mendonça
Published: May 2024

Fire is a natural disturbance in the Cerrado (Brazilian savanna) but its effects on the landscape are capable of altering its pattern of severity and frequency. Because of that, some areas of the Biome can be in dangerous in the National Park of Sempre Vivas, in Minas Gerais State. Within this context, we spatially analyzed fires in areas of Cerrado during the years 2003 and 2017 according to severity, frequency and the influence of the spatial configuration of the area. We used pre- and post-fire Landsat satellite images to calculate the dNBR index to analyze fire severity per year and used fire metrics to analyze the relationship between fire severity and the spatial configuration of the landscape. Fire-scar severity was considered low, probably due to the density and structure of the typical Cerrado vegetation and the change in annual precipitation. Thus, fires in the Cerrado can be intense and spread quickly if no proper control is implemented.
UPREGULATION OF miR-155 IMPAIRS WHITE MATTER SPARING AT THE INJURY AREA FOLLOWING CONTUSIVE SPINAL CORD INJURY IN MICE
Authors: Hussein Hayam A. , Gomaa Mohamed , Deibert Rochelle J. , Fisher Lesley C. , Amici Stephanie A. , Guerau-de-Arellano Mireia , Basso Michele D. , Behery Ahmed E.
Published: May 2024

This study was conducted to characterize the effect of miR-155 overexpression on white matter sparing and lesion size following contusion injury of spinal cord in mice. 60 C57BL/6J wild-type and 60 B6.Cg miR-155 knockout mice were used to induce moderate to severe contusive spinal cord injury at T9 segment. All used mice were female, 8–20 weeks old and weighing 18+ gm. Mice were divided into two main groups; for Real-time reverse transcriptase polymerase chain reaction (RT-PCR) and histology, then subdivided into five subgroups; 1, 3, 7, 14 and 42 days after the contusion according to time point, each was compared to naive control group. Fresh and fixed tissue were taken from thoracic segments (lesion area) from all time points, dissected and then evaluated using RT-PCR and histology (Eriochrome stain), respectively. We identified significant upregulation of miR-155 at the lesion site by 3 days and continued up to 6 weeks after the injury. Following spinal cord injury, the miR-155 overexpression was accompanied with severe cord damage and less tissue repair while more white matter sparing and smaller lesion size were detected in miR-155 knockout group.
EVALUATION OF SPIRULINA PLATENSIS ENRICHED DIET ON GROWTH PERFORMANCE, BIOCHEMICAL PARAMETERS AND IMMUNE RESPONSE OF OREOCHROMIS NILOTICUS
Authors: Abd-El Alim Abd-El Alim F. , Khalifa Hesham A. , El-Murr Abdelhakeem , Magdy Menna Allah
Published: May 2024

Two hundred and twenty five Oreochromis niloticus with average body weight 36±1 g, were distributed randomly into five groups in triplicates. The control group was fed on basal diet, while the other groups were supplemented with various levels of Spirulina platensis (0.5, 1, 1.5 and 2%) for 90 days. The growth performance, biochemical parameters (Alanine aminotransferase activity, Aspartate aminotransferase, Creatinine and cortisol), antioxidant effect (Reduced Glutathion enzyme), IgM and lysozyme were determined. Also, investigating the differences between fish fed the control diet and diet supplemented with Spirulina (1%) against the infection with pathogenic strain of Aeromonas hydrophila. The final body weight, gain percent and specific growth rate was significantly (P˂0.05) increased in fish fed on supplemented diet with Spirulina comparable with that fed on the control diet. The highest final body weight (49.60±0.52g), body gain (11.70±1.04g) and body gain (30.87±4.10%) were recorded in fish fed on diet supplemented with 2% Spirulina. Biochemical, immunological parameters and antioxidant enzyme (GSH) were improved in the group supplemented with Spirulina in comparing with fish fed on basal diet. The best result was recorded in-group fed on Spirulina supplemented diet by level of 1% at which the levels of IgM was 42.40 ±0.66 μg /ml, lysozyme level was 31.33 ±0.44 μg /ml and the level of GSH was 11.7 ±0.39 mg/g tissues. Moreover, Spirulina supplemented with (1%) in diet enhanced fish protection against A. hydrophila infection. The survival rate was (80%) in fish fed on diet supplemented with 1% Spirulina.
INFLUENCE OF GAMETE CO-INCUBATION TIME, SIRE AND SPECIAL ADDITIVES ON THE IN VITRO FERTILIZATION OF CUMULUS-ENCLOSED OR DENUDED BUFFALO OOCYTES
Authors: EL Gebaly Maha , Abdalla Hany , Amer Hussein , Hazza Abu Bakr
Published: May 2024

The present study was designed to investigate the influence of cumulus removal before the in vitro fertilization step and the impact of co-incubation time, sire, and additives to the fertilization medium on the efficiency of in vitro fertilization of buffalo oocytes. In vitro matured oocytes were fertilized either as cumulus-oocyte complex (COCs) or after removal of cumulus cells (denuded). Cumulus-enclosed or denuded oocytes were co-incubated with sperm cells for 6, 12 or 18 h (experiment 1), fertilized with sperm cells from one of three sires (experiment 2) or fertilized in medium supplemented with 20μg∕ml heparin, 5 mM theophylline or a mixture 20μM penicillamine, 10μM hypotaurine and 1 μM epinephrine (PHE) (experiment 3). In all experiments, a group of oocytes was fixed and stained to evaluate the fertilization pattern (penetration, normal and abnormal fertilization rates) and the rest were cultured up to 8 days to assess the developmental competence (cleavage and blastocyst yield). In all experiments, removal of cumulus cells before fertilization step significantly retarded the fertilization pattern and the developmental competence. Various co-incubation times did not significantly influence the fertilization pattern or the developmental competence of denuded or COCs. However, 6 h tended to decrease the abnormal fertilization (15.74±1.70 vs 28.46±6.06, P=0.069) and to improve the blastocyst/oocyte (11.70±3.41 vs 5.53±1.75) and the blastocyst/cleavage (27.14±6.19 vs 11.98±3.81, P=0.082) when compared with 18 h. Sperm cells from the three sires resulted in similar fertilization pattern and developmental competence in COCs and denuded oocytes. In COCs and denuded oocytes, PHE tended to improve the blastocyst/oocyte (7.44±2.58 vs 14.67±4.29) and blastocyst/cleavage (18.43±6.08 vs 33.13±9.27) in comparison to heparin (P˃0.05). Thus, none of the investigated factors could counteract the adverse effect of cumulus removal otherwise, the addition of PHE showed promising results but it need further investigations.
ASPROSIN: A NOVEL BIOMARKER OF TYPE 2 DIABETES MELLITUS
Authors: Elnagar Asmaa , El-Belbasi Hussein I. , Rehan Ibrahim F. , El-Dawy Khalifa
Published: May 2024

Type 2 diabetes mellitus has a deadly toll on human health. Therefore, more attention needs to be paid for the creation of biomarker to indicate the newly diagnostic type 2 diabetes and effective therapy. It well known that adipose tissue of mammals can store energy and secrete such hormones. Therefore, obesity is associated with hyperinsulinemia and insulin resistance. Recently, newly identified glucogenic hormone named “asprosin” has been developed in white adipose tissue, encoded by the gene Fibrillin 1 (Fbn1). Fbn1 is a 230-kb gene with 65 coding exons. These exons encode a 2,871-amino-acid long proprotein called proFibrillin which is proteolytically cleaved near its C-terminus by the enzyme furin convertase to give Fbn1, a member of the fibrillin family, in addition the 140-amino-acid long protein hormone asprosin. It has a metabolic role during fasting condition as it induces liver to secrete glucose for maintaining homeostasis as well as centrally stimulates appetite. It was reported that plasma asprosin concentrations increased in human/mice with type 2 diabetes compared with controls. However, it was significantly reduced in patients with neonatal progeriod syndrome who had characteristic features of low appetite and extreme leanness. This syndrome is due to the truncated mutation in Fbn1. Although the great biological role of asprosin in vivo, it still limited in research, particularly in the therapy of type 2 diabetes. This study aimed to provide an overview of asprosin and its possibility to be used as a novel biomarker of type 2 diabetes and obesity.
Gry cyfrowe i e-sport jako istotny komponent życia współczesnej młodzieży
Authors: Rukat Marta
Published: May 2024

Przedmiotem niniejszego artykułu jest zjawisko angażowania się w gry komputerowe oraz e-sport przez młodych ludzi – a celem wiodącym poszukiwanie odpowiedzi na pytanie o to, co sprawia, że gry komputerowe i e-sport są tak atrakcyjne dla młodzieży? Przedstawiono definicyjne ujęcia e-sportu, opisano pokrótce jego historię oraz przeanalizowano różne aspekty atrakcyjności gamingu - znaczące z perspektywy adolescentów i stanowiące ważny komponent ich życia. Poddano również analizie korzyści, jakie przynosi granie w gry oraz angażowanie się w e-sportowe rozgrywki.
Nowe wyzwania dla teorii edukacji-integralna edukacja oraz mindfulness w edukacji
Authors: Marzec Jarosław
Published: May 2024

Prezentowany tekst odnosi się do tekstu Ewy Rodziewicz, który zawiera upomnienie się o humanistyczna pedagogikę i filozofię edukacji. Zgodnie z tą intencją staram się rozwiązać kwestię odpowiedzialności jako kategorie wiodącą dla humanistycznej edukacji.Moja próba polega na tworzeniu mapy humanizmu i wprowadzeniu koncepcji Kena Wilbera ,,ukrytej gramatyki" oraz mapy dominujących dyskursów edukacyjnych zwłaszcza tych związanych z neoliberalnym podejściem do edukacji. Wprowadzam dwa podejścia, integralną edukację oraz mindfulness w edukacji jako dwa humanistyczne podejścia w odpowiedzi na kulturę ryzyka i kryzys odpowiedzialności w studiach edukacyjnych. Krótko, chce zaproponować te dwa podejścia jako odpowiedź na kwestię podnoszone przez Ewę Rodziewicz.
Analisis Emosi Wisatawan Menggunakan Metode Lexicon Text Analysis
Authors: Rahmadani Dea Caesy , Khomsah Siti , Fathoni M Yoka
Published: May 2024

Wisatawan sering menulis komentar di internet, biasanya tentang pengalaman, pendapat, bahkan keluhan. Banyaknya data komentar wisatawan diinternet dapat memberikan informasi berguna bagi stakeholder. Informasi dari kumpulan komentar dapat diekstrak menggunakan metode text analysis, salah satunya polaritas atau sentimen, umumnya berbentuk sentiment positif dan negatif. Sentimen dapat didetilkan kedalam 8 jenis emosi. Penelitian ini bertujuan mengetahui emosi wisatawab berdasarkan komentar- komentar mereka diakun Google Map objek wisata. Dataset penelitian diambil dari sepuluh objek wisata di BARLINGMASCAKEB. Metode yang digunakan adalah Lexicon Emotion Anlysis. Hasil analisis menunjukan mayoritas wisatawan dari sepuluh objek wisata tersebut menunjukan emosi “bahagia” dan “yakin”. Kata “bahagia” dan “yakin” bermakna positif sehingga bisa dikatakan mayoritas wisatawan menunjukan emosi positif. Objek wisata yang menghadirkan emosi terbanyak pada emosi “bahagia” antara lain: Aquarium Purbasari Pancuran Mas dengan 33,52%,  Lembah Asri Serang  dengan 30,85%, Taman Reptil Sanggaluri sebanyak 30, 27%, Kebun  Raya Baturaden dengan 27, 67%, dan Curug Jenggala sebesar 23,4%.  Sedangkan emosi bahagia yang tertinggi yaitu Benteng Pandem dengan 27, 41%, Candi Arjuna dengan 26,6%, Kawah Sikidang sebesar 20,71%, dan Pantai Menganti sebanyak 25, 74%.  Hanya satu objek wisata yang tanggapi dengan emosi “antisipatif” tertinggi yaitu Taman Miniatur Dunia. Emosi antisipatif diwakili oleh kata-kata tanggapan atau perhatian sehingga masih bisa kategorikan kedalam emosi positif. Ekstraksi emosi sangat ditentukan pada proses pelabelan emosi setiap komentar, maka disarankan mengembangkan kamus emosi lexicon yang digunakan. Hasil penelitian ini diharapkan dapat memberikan manfaat bagi perkembangan industri pariwisata di wilayah BARLINGMASCAKEB dan bagi dunia akademik terutama tentang penerapan text mining pada bidang pariwisata.
Prototype Alat Pemberitahuan Kecelakaan Menggunakan Microcontroller Node MCU dan Sensor Crash
Authors: Natzir Sadam Muhammad , Malahina Edwin Ariesto Umbu
Published: May 2024

Penelitian ini berhasil menghasilkan prototipe alat pemberitahuan kecelakaan otomatis menggunakan Microcontroller Node MCU dan Sensor Crash. Alat ini dirancang untuk mendeteksi insiden kecelakaan dan memberikan respons cepat dengan mengirim notifikasi berisi lokasi kejadian kecelakaan melalui Pesan Singkat (SMS/Telegram) ke unit terkait secara real-time. Sensor Crash dan Node MCU berkolaborasi untuk mendeteksi dan melacak kejadian, sementara sistem penandaan GPS dan modul komunikasi seluler digunakan untuk melacak dan mengirimkan koordinat lokasi kecelakaan. Pengujian prototipe dilakukan dalam berbagai skenario kecelakaan untuk menguji akurasi dan responsifitas sistem. Hasilnya menunjukkan bahwa prototipe ini mampu mendeteksi dan mengirim notifikasi tentang insiden dengan kecepatan dan akurasi yang tinggi. Modul SIM800l digunakan dalam prototipe ini, memiliki tingkat keberhasilan pengiriman pesan sebesar 90%. Penerapan teknologi ini berpotensi meningkatkan efisiensi dan efektivitas respons darurat terhadap kecelakaan, mengurangi waktu penanganan dan potensi kerugian lebih lanjut. Selanjutnya, penelitian ini menunjukkan potensi untuk integrasi lebih lanjut dengan sistem kontrol lalu lintas atau layanan darurat, yang akan membentuk respons yang lebih terkoordinasi dan efektif terhadap insiden kecelakaan.
Effectiveness of Prophylactic Ketamine on Shivering in Cesarean Section after Spinal Anesthesia
Authors: Farooqi Muhammad Hamood , Naeem Kashif , Zafar Aimen , Phull Qamar Zaman , Khan Hira Saeed , Talib Shujaullah
Published: May 2024

Background: Regional anesthesia, including spinal or epidural techniques, can disrupt the body's ability to regulate temperature, leading to a heightened risk of shivering. Studies have indicated that shivering occurs in up to 55% of patients undergoing procedures under regional anesthesia. To address this issue, numerous medications have been explored for their potential to prevent post-anesthetic shivering. Objective: To assess the effectiveness of prophylactic ketamine on shivering in cesarean section after spinal anesthesia. Study Design: Prospective, randomized, double blind, clinical trial.
Settings: Department of Anesthesiology, Surgical Intensive Care Unit and Pain Management, Civil Hospital Karachi Pakistan. Duration: Six months from August 2019 to January 2020.  Methods: A total of 60 patients undergoing spinal anesthesia for elective cesarean section were included in this study. Two groups A and B were formed and patients were equally divided into these groups by lottery method. Group A were treated with ketamine 0.5 mg/kg I/V and group B treated with normal saline 0.05ml/kg/I/V.  Grade 0-1 shivering was labeled as effective and shivering grade 2, 3 or 4 at 15 minutes after administration of the drug, was considered ineffective. Results: The average age of the patients was 28.33 ± 5.67 Years. Rate of shivering was significantly high in patients who were treated with normal saline than ketamine in which shivering was not observed (53.3 vs. 0% respectively; P value of 0.0005). This implies that effectiveness was significantly high in groups A than group B (100% vs. 46.7%). Conclusion: Ketamine observed to be the effective drug for the treatment of postanesthetic shivering. It should be considered prophylactically in any patient undergoing spinal anesthesia.
Impact of Work Overload on Nurses Performance in A Tertiary Care Hospital Lahore
Authors: Naveed Azil , Farooq Zambeel , Tariq Mariam , Siddique Alia , Altaf Hira , Noreen Naila , Zafar Misbah
Published: May 2024

Background: Workload is increased by nurses' intellectual obligations as well as physical ones. Workload compromises worth of life in nurses. Nurses' performance needs up-to-date knowledge and virtuous managerial skills for patient safety and along with compassionate nursing care. Objective: To determine impact of work overload on nurses performance in a tertiary care hospital Lahore. Study Design: Quantitative descriptive study design was applied to determine impact of workload on nurses’ performances. Settings: Services Institute of Medical Sciences (SIMS), Lahore Pakistan. Duration: 6 moths from June 2022 to December 2022. Methods: 146 nurses participated and data collection was done by performance questionnaire. Nurses Age>25 and who were working in all departments of SIMS, Services Hospital Lahore. Nursing students were excluded. Facts collection permission was taken from the nursing superintendent and medical superintendent of Services Institute of Medical Sciences (SIMS) Services Hospital Lahore. Informed consent was taken by the participants in the study. Data was gathered by questionnaires of workload and nurses performances. Results: Results revealed that above 70% of participants showed that their performance increased during work related stress although quality care compromised but reflexes increased. They were able to manage the patient in very short time. Conclusion: This study concluded that there is positive, constructive and unidirectional impact of workload on nurses’ performance in a tertiary care hospital Lahore.
delito de cohecho en el sistema penal ecuatoriano
Authors: Aguilera Quezada Jorge
Published: May 2024

El presente trabajo tiene como objeto realizar un estudio pormenorizado del tipo penal de cohecho, delito que se encuentra previsto en el artículo 280 del Código Orgánico Integral Penal como una de las conductas que atentan contra la eficiencia de la administración pública, bajo la luz de la doctrina autorizada y de acuerdo a la normativa ecuatoriana. En el último tiempo, en el país se ha debatido arduamente en torno a esta figura, debido a casos que involucran a altos funcionarios de gobiernos pasados y el actual, sin que se conozca verdaderamente su naturaleza y características especiales, por lo que este artículo tiene como propósito desentrañar sus particularidades para una mejor comprensión de esta.
responsabilidad sobre la reparación del daño por el delito de femicidio en casos de fallecimiento de victimarios en el Ecuador
Authors: Urresta Merino Andrés Sebastián
Published: May 2024

.Según las estadísticas oficiales de 2022, Ecuador enfrenta la cruda realidad de un feminicidio que ocurre aproximadamente cada 26 horas, lo que indica una urgente necesidad de políticas públicas efectivas y un sistema de justicia sólido. Las recientes crisis carcelarias y la violencia creciente subrayan la urgencia de abordar este problema, lo que lleva a un examen exhaustivo de la responsabilidad por el daño y la compensación a las víctimas, especialmente en casos donde los perpetradores evaden la justicia. A nivel internacional, la reparación integral, según la Comisión Interamericana de Derechos Humanos (CIDH), va más allá de la mera compensación económica, abarcando medidas tangibles y simbólicas para mitigar el daño. En Ecuador, los mecanismos de reparación integral sirven como herramientas vitales a las autoridades judiciales para atender las necesidades de las víctimas, independientemente de las circunstancias o disposición del perpetrador para ofrecer restitución. Como se detalla en el artículo 78 del Código Orgánico Integral Penal y la Constitución, la compensación emerge como un aspecto crucial, centrándose principalmente en pérdidas tangibles y reparaciones monetarias cuantificables. Los tribunales judiciales distinguen entre indemnización, dirigida a daños materiales y pérdidas de ganancias, y compensación, que aborda el daño intangible como el sufrimiento psicológico y emocional. Este enfoque dual garantiza una respuesta integral a los impactos multifacéticos de la violencia, abarcando tanto aspectos medibles como subjetivos de la victimización.
Practice of work of a nurse in organizing care for patients with diabetes mellitus: training programs «from innovation to quality of life» in the conditions of the Union State
Authors: Petryaykina E.E. , Grigoriev K.I. , Matveychik T.V. , Vykhristyuk O.F.
Published: May 2024

Help and treatment of children and adolescents with diabetes is an important part of the health care system. A socially oriented state is obliged to provide this category of patients with the development and application of the latest methods in the field of preventive diabetology and medical demography, and is obliged to form and educate the correct behavior of children and adolescents, which must be implemented in the family, school, and educational organizations. Of great importance is the use of standards/algorithms for specialized medical care for patients with diabetes, educational programs designed not only to provide knowledge about the disease, but also to teach patients the skills of self-monitoring of the disease, the principles of independent decision-making on treatment and prevention. The article outlines the modern principles of organizing work in “Health Schools” and “Schools of Diabetes Mellitus”, educational programs used by medical workers, and the achieved results of activities in the field of preventing exacerbations of diabetes mellitus in parallel mode both in Russia and Belarus.