Cemaran Mikroba Pada Telur Penyu Sisik (Eretmochelys imbricata) di Pulau Kelapa Dua, Taman Nasional Laut Kepulauan Seribu, DKI Jakarta
Authors: Wicaksono Mukti Ageng , Nurhasanah Fitriani , Elfidasari Dewi , Sugoro Irawan 
Published: November 2019

<div class="WordSection1"><p><em>Abstrak</em> – <strong>Penyu sisik (<em>Eretmochelysimbricata</em>) merupakan</strong><strong> </strong><strong>salah</strong><strong> </strong><strong>satu</strong><strong> jenis penyu yang hidup di perairan Indonesia. Populasi penyu sisik saat ini terus mengalami penurunan. </strong><strong>Salah satu</strong><strong> </strong><strong>fa</strong><strong>k</strong><strong>tor</strong><strong> </strong><strong>penyebab</strong><strong>nya </strong><strong>adalah</strong><strong> </strong><strong>keberadaan</strong><strong> </strong><strong>mikroba</strong><strong> </strong><strong>pencemar</strong><strong> </strong><strong>pada</strong><strong> </strong><strong>telur</strong><strong> </strong><strong>penyu.</strong><strong> </strong><strong>Tujuan</strong><strong> </strong><strong>dari</strong><strong> </strong><strong>penelitian</strong><strong> </strong><strong>ini</strong><strong> </strong><strong>adalah</strong><strong> </strong><strong>mengetahui</strong><strong> </strong><strong>cemaran</strong><strong> </strong><strong>mikroba</strong><strong> </strong><strong>pada</strong><strong> </strong><strong>telur</strong><strong> </strong><strong>penyu</strong><strong> </strong><strong>sisik di Pulau</strong><strong> </strong><strong>Kelapa</strong><strong> </strong><strong>Dua.</strong><strong> </strong><strong>Analisis</strong><strong> </strong><strong>secara</strong><strong> </strong><strong>mikrobiologi</strong><strong> meliputi </strong><strong>total bakteri, jamur, </strong><strong>c</strong><strong>oliform, <em>Enterobacter</em>, dan</strong><strong> </strong><strong><em>Salmonella-Shigella</em></strong><strong><em> </em></strong><strong>dilakukan</strong><strong> </strong><strong>terhadap</strong><strong> </strong><strong>sampel</strong><strong> </strong><strong>telur</strong><strong> </strong><strong>segar</strong><strong> </strong><strong>dan</strong><strong> telur </strong><strong>gagal</strong><strong> </strong><strong>menetas.</strong><strong> </strong><strong>Hasil</strong><strong> </strong><strong>analisis</strong><strong> </strong><strong>menunjukkan</strong><strong> </strong><strong>bahwa</strong><strong> </strong><strong>semua</strong><strong> </strong><strong>sampel</strong><strong> </strong><strong>tercemar</strong><strong> </strong><strong>mikroba</strong><strong> yang mempengaruhi daya tetas telur. </strong><strong>Sampel</strong><strong> </strong><strong>cangkang</strong><strong> </strong><strong>telur</strong><strong> </strong><strong>segar</strong><strong> </strong><strong>memiliki total bakteri</strong><strong> </strong><strong>9,60x10<sup>5</sup> CFU/butir</strong><strong>,</strong><strong> </strong><strong> jamur</strong><strong> </strong><strong>2,35x10<sup>5</sup> </strong><strong>propagul</strong><strong>/butir</strong><strong>, coliform</strong><strong> </strong><strong>3,96x10<sup>5</sup> CFU/butir</strong><strong>, <em>Enterobacter</em></strong><strong><em> </em></strong><strong>3,02x10<sup>5</sup> CFU/butir</strong><strong> </strong><strong>d</strong><strong>an</strong><strong> </strong><strong><em>Salmonella-Shigella</em></strong><strong><em> </em></strong><strong>1,68x10<sup>5</sup> CFU/butir</strong><strong>, sedangkan</strong><strong> </strong><strong>hasil</strong><strong> </strong><strong>analisis</strong><strong> </strong><strong>dari</strong><strong> </strong><strong>sampel</strong><strong> </strong><strong>isi</strong><strong> </strong><strong>telur</strong><strong> </strong><strong>segar</strong><strong> </strong><strong>diperoleh</strong><strong> </strong><strong>total bakteri</strong><strong> </strong><strong>1,60x10<sup>4</sup></strong><strong></strong><strong>CFU/ml</strong><strong>,</strong><strong> </strong><strong>jamur</strong><strong> </strong><strong>3,00x10<sup>2</sup></strong><strong></strong><strong>propagul</strong><strong>/ml</strong><strong>, coliform</strong><strong> </strong><strong>2,00x10<sup>5</sup></strong><strong></strong><strong>CFU/ml</strong><strong>, <em>Enterobacter</em></strong><strong><em> </em></strong><strong>1,20x10<sup>5</sup></strong><strong></strong><strong>CFU/ml</strong><strong> </strong><strong>d</strong><strong>an</strong><strong> </strong><strong><em>Salmonella-Shigella</em></strong><strong><em> </em></strong><strong>1,46x10<sup>5</sup></strong><strong></strong><strong>CFU/ml.</strong><strong> </strong><strong>Hasil</strong><strong> </strong><strong>analisis</strong><strong> </strong><strong>dari</strong><strong> </strong><strong>sampel</strong><strong> </strong><strong>cangkang</strong><strong> </strong><strong>telur</strong><strong> </strong><strong>gagal</strong><strong> </strong><strong>menetas</strong><strong> </strong><strong>diperoleh</strong><strong> </strong><strong>total bakteri 7,20x10<sup>6</sup></strong><strong></strong><strong>CFU/butir</strong><strong>,</strong><strong> </strong><strong>jamur</strong><strong> </strong><strong>1,92x10<sup>5</sup></strong><strong> </strong><strong>propagul</strong><strong>/butir</strong><strong>, coliform</strong><strong> </strong><strong>4,08x10<sup>5</sup></strong><strong> </strong><strong>CFU/butir</strong><strong>, <em>Enterobacter</em></strong><strong><em> </em></strong><strong>2,59x10<sup>5</sup></strong><strong></strong><strong>CFU/butir</strong><strong> </strong><strong>d</strong><strong>an</strong><strong> </strong><strong><em>Salmonella-Shigella</em></strong><strong><em> </em></strong><strong>3,31x10<sup>5</sup></strong><strong></strong><strong>CFU/butir</strong><strong>, sedangkan</strong><strong> </strong><strong>hasil</strong><strong> </strong><strong>analisis</strong><strong> </strong><strong>dari</strong><strong> </strong><strong>sampel</strong><strong> </strong><strong>isi</strong><strong> </strong><strong>telur</strong><strong> </strong><strong>gagal</strong><strong> </strong><strong>menetas</strong><strong> </strong><strong>diperoleh total bakteri</strong><strong> </strong><strong>1,00x10<sup>8</sup></strong><strong></strong><strong>CFU/ml</strong><strong>, jamur</strong><strong> </strong><strong>3,50x10<sup>5</sup></strong><strong></strong><strong>propagul</strong><strong>/ml</strong><strong>, coliform</strong><strong> </strong><strong>5,00x10<sup>3</sup></strong><strong></strong><strong>CFU/ml</strong><strong>, <em>Enterobacter</em></strong><strong><em> </em></strong><strong>4,00x10<sup>3</sup></strong><strong></strong><strong>CFU/ml d</strong><strong>an</strong><strong> </strong><strong><em>Salmonella-Shigella</em></strong><strong><em> </em></strong><strong>3,00x10<sup>3</sup></strong><strong></strong><strong>CFU/ml.</strong><strong></strong></p><p><strong><em>Kata Kunci</em></strong> - <em>Penyu</em><em> </em><em>sisik,</em><em> </em><em>telur, Pulau</em><em> K</em><em>elapa</em><em> </em><em>Dua, cemaran</em><em> </em><em>mikroba.</em></p><p> </p><p><em>Abstract</em> - <strong>Hawksbill</strong><strong> (<em>Eretmochelysimbricata</em>) is one of the turtle species that live in Indonesian waters. The current population of hawksbill continues to decline</strong><strong>. One of the contributing factors is the presence of contaminant microbes in turtle eggs. The purpose of this research is to know microbial contamination on hawksbill eggs in Kelapa Dua Island. Microbiological analyzes included total bacteria, fungi, coliform, <em>Enterobacter</em>, and <em>Salmonella-Shigella</em> were performed on fresh egg samples and eggs failed to hatch. The results showed that all samples were contaminated with microbes affecting the hatchability of the eggs. Fresh eggshell samples had total bacteria of 9.60x105 CFU/grains, 2.35x105 fungus propagules/grains, coliform 3.96x105 CFU/grains, <em>Enterobacter</em> 3.02x105 CFU/grains and <em>Salmonella-Shigella</em> 1.68x105 CFU/grains, while yield analysis of fresh egg contents samples obtained total bacteria 1.60x104 CFU/ml, mushrooms 3,00x102 propagules/ml, coliform 2.00x105 CFU/ml, <em>Enterobacter</em> 1,20x105 CFU/ml and <em>Salmonella-Shigella</em> 1,46x105 CFU/ml. Results of analysis of eggshell samples failed to hatch obtained total bacteria 7.20x106 CFU/grains, 1.92x105 fungi propagules/grains, coliform 4.08x105 CFU/grains, <em>Enterobacter</em> 2.59x105 CFU/grains and <em>Salmonella-Shigella</em> 3.31x105 CFU/while the results of the analysis of the sample of egg contents failed to hatch obtained total bacteria 1.00x108 CFU/ml, mushrooms 3,50x105 propagules/ml, coliform 5.00x103 CFU/ml, <em>Enterobacter </em>4,00x103 CFU/ml and <em>Salmonella-Shigella</em> 3, 00x103 CFU/ml. </strong></p><p><strong> </strong></p><p><strong><em>Keywords</em></strong> – <em>Hawksbill, Egg, Kelapa Dua Island, microbial contamination</em></p></div>