bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiphase Acoustic Channel Characterization for Downhole Communication
Authors: Ahmad Talha J , Arsalan Muhammad , Noui-Mehidi Mohamed N , Black Michael
Published: December 2015

Reservoir management and production optimization relies on acquiring reservoir data captured by different sensors, and adjusting fluid flow rate in laterals using flow control devices, all of which are permanently installed as part of a smart completion. One of the important challenges for the next generation of smart completions for advanced well architectures is downhole wireless communication, since umbilicals are undesirable because of high cost, reliability issues, complicated installation and maintenance. Although a number of technologies exist for wireless communication in vertical sections of the well using a tubular conduit (production tubing, casing) as the transmission medium, there is an absence of wireless communication method in the horizontal openhole section of the well.

This study investigates acoustics for wireless communication through multiphase flow in a pipeline. A laboratory test setup was built to investigate acoustic propagation through two-phase flow. The setup consists of a laboratory multiphase flow rig, acoustic transceivers, a digital communication system, and required electronics circuitry. The effect of various flow and communication parameters on acoustic data communication is studied.

The study shows that different multiphase flow parameters affect various distortion phenomena, including absorption, dispersion and ambient noise, which significantly impacts acoustic communication. Also the frequency content and spectrum of the transmitted signal affects the bit rate and communication range. The acoustic beam width and beam angle also has an important effect on dispersion and multipath propagation. Multiphase channel has a dispersive and non-linear nature and to reduce distortion phenomena and achieve better communication in flow, a number of advanced signal processing and communication techniques are proposed.
Taking Advantage of Web-GIS Technologies to Globally Manage Petroleum Asset Information
Authors: Amouroux Jean-Michel , Leclerc Jean-Charles
Published: December 2015

The ability to access and share one valid and up-to-date technical information is a key element of our efficiency with the follow-up of our projects and operations. This will have a critical impact in case of incidents and/or crisis situations, as this could happen in sensitive areas like in the Middle-East or West Africa.

In case of a major crisis, we must be able to restore the access to the GIS data identified as critical within a time limit of 4 to 8 hours!
Amplification strategy to enhance speech perception in individuals with auditory neuropathy spectrum disorder
Authors: Barman Animesh , Sinha Sujeet Kumar , Prabhu Prashanth
Published: December 2015

Objective:

The present study was conducted to determine if low-cut modified amplification improved aided speech perception compared to standard NAL-NL1 prescriptive amplification in individuals with ANSD. The study further aimed to check the influence of the number of channels in a hearing aid on speech perception with low-cut modification of amplification.

Study design:

A total of 22 individuals with ANSD in the age range 15–42 years were recruited for the study. The unaided and aided speech identification scores were obtained with standard amplification and low-cut amplification settings in both a four- and a 16-channel digital hearing aid.

Results:

The results showed that low-cut amplification was slightly better than standard amplification for aided speech perception. Such an improvement could be attributed to the elimination of upward spread of masking during low-cut modification of amplification. In addition, improvement was greater with a four-channel hearing aid compared to a 16-channel hearing aid.

Conclusions:

The use of low-cut amplification with a low number of channels in the hearing aid for individuals with ANSD is recommended. However, further studies are essential to determine whether the slight improvement in aided scores with a low-cut fitting strategy leads to actual improvement in quality of life.
Christianity and Sports: Religious Functionaries and Charismatic Prophets in Ghana Soccer
Authors: Asamoah-Gyadu J. Kwabena
Published: December 2015

This paper examines the way in which, within an African religious and spiritual context, athletes – and in particular footballers of Ghana – employ religious functionaries and religious means from a variety of traditions in an attempt to achieve sporting success. Specific examples and case studies illustrate and contextualise this search. The connections of this mode of searching for success with traditional African views of causality and with a Pentecostalist/charismatic prosperity ethic are explored, and its consequences are assessed.
VGLUTs and Glutamate Synthesis—Focus on DRG Neurons and Pain
Authors: Malet Mariana , Brumovsky Pablo
Published: December 2015

The amino acid glutamate is the principal excitatory transmitter in the nervous system, including in sensory neurons that convey pain sensation from the periphery to the brain. It is now well established that a family of membrane proteins, termed vesicular glutamate transporters (VGLUTs), serve a critical function in these neurons: they incorporate glutamate into synaptic vesicles. VGLUTs have a central role both under normal neurotransmission and pathological conditions, such as neuropathic or inflammatory pain. In the present short review, we will address VGLUTs in the context of primary afferent neurons. We will focus on the role of VGLUTs in pain triggered by noxious stimuli, peripheral nerve injury, and tissue inflammation, as mostly explored in transgenic mice. The possible interplay between glutamate biosynthesis and VGLUT-dependent packaging in synaptic vesicles, and its potential impact in various pain states will be presented.
Unit Commitment Model Considering Flexible Scheduling of Demand Response for High Wind Integration
Authors: Wang Beibei , Liu Xiaocong , Zhu Feng , Hu Xiaoqing , Ji Wenlu , Yang Shengchun , Wang Ke , Feng Shuhai
Published: December 2015

In this paper, a two-stage stochastic unit commitment (UC) model considering flexible scheduling of demand response (DR) is proposed. In the proposed UC model, the DR resources can be scheduled: (1) in the first stage, as resources on a day-ahead basis to integrate the predicted wind fluctuation with lower uncertainty; (2) in the second stage, as resources on an intra-day basis to compensate for the deviation among multiple wind power scenarios considering the coupling relationship of DR on available time and capacity. Simulation results on the Pennsylvania-New Jersey-Maryland (PJM) 5-bus system and IEEE 118-bus system indicate that the proposed model can maximize the DR value with lower cost. Moreover, different types of DR resources may vary in the contract costs (capacity costs), the responsive costs (energy costs), the time of advance notice, and the minimum on-site hours. The responsive cost is considered as the most important factor affecting DR scheduling. In addition, the first-stage DR is dispatched more frequently when transmission constraints congestion occurs.
Awareness of Cytomegalovirus Infection among Pregnant Women in Geneva, Switzerland: A Cross-sectional Study
Authors: Willame Alexia , Blanchard-Rohner Geraldine , Combescure Christophe , Irion Olivier , Posfay-Barbe Klara , Martinez de Tejada Begoña
Published: December 2015

Background: Cytomegalovirus (CMV) is the most frequent cause of congenital infection and commonly associated with sensorineural deficit. At present, there is neither prophylaxis nor treatment during pregnancy. The objective of this study was to evaluate the level of awareness regarding CMV infection and its consequences in women delivering at the University of Geneva Hospitals (Geneva, Switzerland). Methods: The study consisted of a validated questionnaire completed by women in the immediate postpartum period. Results: The questionnaire was completed by 59% (314/528) of delivering women. Only 39% (123/314) knew about CMV and 19.7% (62/314) had received information about preventive measures. Women were more aware about other congenital diseases, such as toxoplasmosis (87%); human immunodeficiency virus (99%); syphilis (85.5%); rubella (92.3%); and group B Streptococcus (63%). Factors associated with CMV awareness were Swiss nationality, high education level, employment in health care or with children, and being followed by an obstetrician. Regarding quality of information, few were aware of the main CMV complications (deafness, 25.2%; mental retardation, 34.5%). Among those informed about CMV, most (74.6%) knew about preventive measures. Among these, 82.5% thought that these were easily applicable. Conclusions: Most women were unaware of CMV infection and its potential risks during pregnancy. It is crucial to improve CMV information given to pregnant women to prevent the risks for the fetus/newborn.
Effects of 90-Day Feeding of Transgenic Maize BT799 on the Reproductive System in Male Wistar Rats
Authors: Guo Qian-ying , He Li-xia , Zhu Han , Shang Jun-li , Zhu Ling-yan , Wang Jun-bo , Li Yong
Published: December 2015

BT799 is a genetically modified (GM) maize plant that expresses the Cry1Ac gene from Bacillus thuringiensis (Bt). The Cry1Ac gene was introduced into maize line Zhen58 to encode the Bt crystal protein and thus produce insect-resistant maize BT799. Expression of Bt protein in planta confers resistance to Lepidopteran pests and corn rootworms. The present study was designed to investigate any potential effects of BT799 on the reproductive system of male rats and evaluate the nutritional value of diets containing BT799 maize grain in a 90-day subchronic rodent feeding study. Male Wistar rats were fed with diets containing BT799 maize flours or made from its near isogenic control (Zhen58) at a concentration of 84.7%, nutritionally equal to the standard AIN-93G diet. Another blank control group of male rats were treated with commercial AIN-93G diet. No significant differences in body weight, hematology and serum chemistry results were observed between rats fed with the diets containing transgenic BT799, Zhen58 and the control in this 13-week feeding study. Results of serum hormone levels, sperm parameters and relative organ/body weights indicated no treatment-related side effects on the reproductive system of male rats. In addition, no diet-related changes were found in necropsy and histopathology examinations. Based on results of the current study, we did not find any differences in the parameters tested in our study of the reproductive system of male rats between BT799 and Zhen58 or the control.
Parallel Reaction Monitoring: A Targeted Experiment Performed Using High Resolution and High Mass Accuracy Mass Spectrometry
Authors: Rauniyar Navin
Published: December 2015

The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review discusses the label-based and label-free methods of quantification that can be performed with the targeted approach.
Plants as Factories for Human Pharmaceuticals: Applications and Challenges
Authors: Yao Jian , Weng Yunqi , Dickey Alexia , Wang Kevin
Published: December 2015

Plant molecular farming (PMF), defined as the practice of using plants to produce human therapeutic proteins, has received worldwide interest. PMF has grown and advanced considerably over the past two decades. A number of therapeutic proteins have been produced in plants, some of which have been through pre-clinical or clinical trials and are close to commercialization. Plants have the potential to mass-produce pharmaceutical products with less cost than traditional methods. Tobacco-derived antibodies have been tested and used to combat the Ebola outbreak in Africa. Genetically engineered immunoadhesin (DPP4-Fc) produced in green plants has been shown to be able to bind to MERS-CoV (Middle East Respiratory Syndrome), preventing the virus from infecting lung cells. Biosafety concerns (such as pollen contamination and immunogenicity of plant-specific glycans) and costly downstream extraction and purification requirements, however, have hampered PMF production from moving from the laboratory to industrial application. In this review, the challenges and opportunities of PMF are discussed. Topics addressed include; transformation and expression systems, plant bioreactors, safety concerns, and various opportunities to produce topical applications and health supplements.
Current Status of Herbal Medicines in Chronic Liver Disease Therapy: The Biological Effects, Molecular Targets and Future Prospects
Authors: Hong Ming , Li Sha , Tan Hor , Wang Ning , Tsao Sai-Wah , Feng Yibin
Published: December 2015

Chronic liver dysfunction or injury is a serious health problem worldwide. Chronic liver disease involves a wide range of liver pathologies that include fatty liver, hepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. The efficiency of current synthetic agents in treating chronic liver disease is not satisfactory and they have undesirable side effects. Thereby, numerous medicinal herbs and phytochemicals have been investigated as complementary and alternative treatments for chronic liver diseases. Since some herbal products have already been used for the management of liver diseases in some countries or regions, a systematic review on these herbal medicines for chronic liver disease is urgently needed. Herein, we conducted a review describing the potential role, pharmacological studies and molecular mechanisms of several commonly used medicinal herbs and phytochemicals for chronic liver diseases treatment. Their potential toxicity and side effects were also discussed. Several herbal formulae and their biological effects in chronic liver disease treatment as well as the underlying molecular mechanisms are also summarized in this paper. This review article is a comprehensive and systematic analysis of our current knowledge of the conventional medicinal herbs and phytochemicals in treating chronic liver diseases and on the potential pitfalls which need to be addressed in future study.
The Biological Diversity and Production of Volatile Organic Compounds by Stem-Inhabiting Endophytic Fungi of Ecuador
Authors: Rundell Susan , Spakowicz Daniel , Narváez-Trujillo Alexandra , Strobel Scott
Published: December 2015

Fungal endophytes colonize every major lineage of land plants without causing apparent harm to their hosts. Despite their production of interesting and potentially novel compounds, endophytes—particularly those inhabiting stem tissues—are still a vastly underexplored component of microbial diversity. In this study, we explored the diversity of over 1500 fungal endophyte isolates collected from three Ecuadorian ecosystems: lowland tropical forest, cloud forest, and coastal dry forest. We sought to determine whether Ecuador’s fungal endophytes are hyperdiverse, and whether that biological diversity is reflected in the endophytes’ chemical diversity. To assess this chemical diversity, we analyzed a subset of isolates for their production of volatile organic compounds (VOCs), a representative class of natural products. This study yielded a total of 1526 fungal ITS sequences comprising some 315 operational taxonomic units (OTUs), resulting in a non-asymptotic OTU accumulation curve and characterized by a Fisher’s α of 120 and a Shannon Diversity score of 7.56. These figures suggest that the Ecuadorian endophytes are hyperdiverse. Furthermore, the 113 isolates screened for VOCs produced more than 140 unique compounds. These results present a mere snapshot of the remarkable biological and chemical diversity of stem-inhabiting endophytic fungi from a single neotropical country.
4-[3-(Pyridin-3-yl)prop-2-enoyl]phenyl 4-dodecyloxybenzoate
Authors: Ha Sie-Tiong , Lau Yi-Jun
Published: December 2015

Steglich esterification between 1-(4-hydroxyphenyl)-3-(pyridin-3-yl)prop-2-en-1-one and 4-dodecyloxybenzoic acid has produced a new compound, 4-[3-(pyridin-3-yl) prop-2-enoyl]phenyl 4-dodecyloxybenzoate. The title compound was characterized by IR and NMR analysis.
An Investigation of the Microstructure of an Intermetallic Layer in Welding Aluminum Alloys to Steel by MIG Process
Authors: Nguyen Quoc , Huang Shyh-Chour
Published: December 2015

Butt joints of A5052 aluminum alloy and SS400 steel, with a new type of chamfered edge, are welded by means of metal inert gas welding and ER4043 Al-Si filler metal. The microhardness and microstructure of the joint are investigated. An intermetallic layer is found on the surface of the welding seam and SS400 steel sheet. The hardness of the intermetallic layer is examined using the Vickers hardness test. The average hardness values at the Intermetallic (IMC) layer zone and without the IMC layer zone were higher than that of the welding wire ER4043. The tensile strength test showed a fracture at the intermetallic layer when the tensile strength is 225.9 MPa. The tensile value test indicated the average of welds was equivalent to the 85% tensile strength of the A5052 aluminum alloy. The thickness of the intermetallic layers is non-uniform at different positions with the ranges from 1.95 to 5 μm. The quality of the butt joint is better if the intermetallic layer is minimized. The Si crystals which appeared at the welding seam, indicating that this element participated actively during the welding process, also contributed to the IMC layer’s formation.
Physical Characteristics of Medical Textile Prostheses Designed for Hernia Repair: A Comprehensive Analysis of Select Commercial Devices
Authors: Miao Linli , Wang Fang , Wang Lu , Zou Ting , Brochu Gaétan , Guidoin Robert
Published: December 2015

Inguinal hernia repairs are among the most frequent operations performed worldwide. This study aims to provide further understanding of structural characteristics of hernia prostheses, and better comprehensive evaluation. Weight, porosity, pore size and other physical characteristics were evaluated; warp knitting structures were thoroughly discussed. Two methods referring to ISO 7198:1998, i.e., weight method and area method, were employed to calculate porosity. Porosity ranged from 37.3% to 69.7% measured by the area method, and 81.1% to 89.6% by the weight method. Devices with two-guide bar structures displayed both higher porosity (57.7%–69.7%) and effective porosity (30.8%–60.1%) than single-guide bar structure (37.3%–62.4% and 0%–5.9%, respectively). Filament diameter, stitch density and loop structure combined determined the thickness, weight and characteristics of pores. They must be well designed to avoid zero effective porosity regarding a single-bar structure. The area method was more effective in characterizing flat sheet meshes while the weight method was perhaps more accurate in describing stereoscopic void space for 3D structure devices. This article will give instructive clues for engineers to improve mesh structures, and better understanding of warp knitting meshes for surgeons.
Electrical Properties and Interfacial Studies of HfxTi1–xO2 High Permittivity Gate Insulators Deposited on Germanium Substrates
Authors: Lu Qifeng , Mu Yifei , Roberts Joseph , Althobaiti Mohammed , Dhanak Vinod , Wu Jingjin , Zhao Chun , Zhao Ce , Zhang Qian , Yang Li , Mitrovic Ivona , Taylor Stephen , Chalker Paul
Published: December 2015

In this research, the hafnium titanate oxide thin films, TixHf1–xO2, with titanium contents of x = 0, 0.25, 0.9, and 1 were deposited on germanium substrates by atomic layer deposition (ALD) at 300 °C. The approximate deposition rates of 0.2 Å and 0.17 Å per cycle were obtained for titanium oxide and hafnium oxide, respectively. X-ray Photoelectron Spectroscopy (XPS) indicates the formation of GeOx and germanate at the interface. X-ray diffraction (XRD) indicates that all the thin films remain amorphous for this deposition condition. The surface roughness was analyzed using an atomic force microscope (AFM) for each sample. The electrical characterization shows very low hysteresis between ramp up and ramp down of the Capacitance-Voltage (CV) and the curves are indicative of low trap densities. A relatively large leakage current is observed and the lowest leakage current among the four samples is about 1 mA/cm2 at a bias of 0.5 V for a Ti0.9Hf0.1O2 sample. The large leakage current is partially attributed to the deterioration of the interface between Ge and TixHf1–xO2 caused by the oxidation source from HfO2. Consideration of the energy band diagrams for the different materials systems also provides a possible explanation for the observed leakage current behavior.
Phononic Band Gaps in 2D Quadratic and 3D Cubic Cellular Structures
Authors: Warmuth Franziska , Körner Carolin
Published: December 2015

The static and dynamic mechanical behaviour of cellular materials can be designed by the architecture of the underlying unit cell. In this paper, the phononic band structure of 2D and 3D cellular structures is investigated. It is shown how the geometry of the unit cell influences the band structure and eventually leads to full band gaps. The mechanism leading to full band gaps is elucidated. Based on this knowledge, a 3D cellular structure with a broad full band gap is identified. Furthermore, the dependence of the width of the gap on the geometry parameters of the unit cell is presented.
Novel Topologies in Vanadium-bis-β-Diketone Chemistry: A [V4] and a [V6] Metallacyclophane
Authors: Borilovic Ivana , Roubeau Olivier , Fernández Vidal Irene , Teat Simon , Aromí Guillem
Published: December 2015

Exploring the chemistry of vanadyl ions (VO2+) with bis-β-diketone ligands, in pyridine reactions of vanadyl sulfate with 1,3-bis-(3-oxo-3-(2-hydroxyphenyl)-propionyl)-benzene (H4L1) and 1,3-bis-(3-oxo-3-(2-hydroxyphenyl)-propionyl)-pyridine (H4L2), two novel clusters, [(VIVO)4(H2L1)4(py)4] (1) and [(VVO)4(VIVO)2 (O)4(L2)2(py)6] (2) were prepared and characterized. Due to the conformational flexibility of the ligands, both entities exhibit very peculiar metal topologies and composition, differing significantly from structural patterns established in the related chemistry of divalent 3d metals. Structural analysis also unveils the existence of the most complex metallamacrocycles from this family to date. Studies of the magnetic properties via bulk magnetization measurements and EPR spectroscopy confirmed the existence of uncoupled long-distant S = 1/2 metal centers and the spin ground states S = 2 and S = 1 of the clusters.
Susceptibility of Select Agents to Predation by Predatory Bacteria
Authors: Russo Riccardo , Chae Richard , Mukherjee Somdatta , Singleton Eric , Occi James , Kadouri Daniel , Connell Nancy
Published: December 2015

Select Agents are microorganisms and toxins considered to be exploitable as biological weapons. Although infections by many Select Agents can be treated by conventional antibiotics, the risk of an emerging or engineered drug resistant strain is of great concern. One group of microorganisms that is showing potential to control drug resistant Gram-negative bacteria are the predatory bacteria from the genera Bdellovibrio spp. and Micavibrio spp. In this study, we have examined the ability of Bdellovibrio bacteriovorus (B. bacteriovorus) strain 109J, HD100 and Micavibrio aeruginosavorus (M. aeruginosavorus) ARL-13 to prey on a variety of Select Agents. Our findings demonstrate that B. bacteriovorus and M. aeruginosavorus are able to prey efficiently on Yersinia pestis and Burkholderia mallei. Modest predation was also measured in co-cultures of B. bacteriovorus and Francisella tularensis. However, neither of the predators showed predation when Burkholderia pseudomallei and Brucella melitensis were used as prey.
Soluble Epoxide Hydrolase Inhibitory Activity of Selaginellin Derivatives from Selaginella tamariscina
Authors: Kim Jang , Cho Chong , Tai Bui , Yang Seo , Choi Gug-seoun , Kang Jong , Kim Young
Published: December 2015

Selaginellin derivatives 1–3 isolated from Selaginella tamariscina were evaluated for their inhibition of soluble epoxide hydrolase (sEH) to demonstrate their potential for the treatment of cardiovascular disease. All selaginellin derivatives (1–3) inhibited sEH enzymatic activity and PHOME hydrolysis, in a dose-dependent manner, with IC50 values of 3.1 ± 0.1, 8.2 ± 2.2, and 4.2 ± 0.2 μM, respectively. We further determined that the derivatives function as non-competitive inhibitors. Moreover, the predicted that binding sites and interaction between 1–3 and sEH were solved by docking simulations. According to quantitative analysis, 1–3 were confirmed to have high content in the roots of S. tamariscina; among them, selaginellin 3 exhibited the highest content of 189.3 ± 0.0 μg/g.
Accurate Mass GC/LC-Quadrupole Time of Flight Mass Spectrometry Analysis of Fatty Acids and Triacylglycerols of Spicy Fruits from the Apiaceae Family
Authors: Nguyen Thao , Aparicio Mario , Saleh Mahmoud
Published: December 2015

The triacylglycerol (TAG) structure and the regio-stereospecific distribution of fatty acids (FA) of seed oils from most of the Apiaceae family are not well documented. The TAG structure ultimately determines the final physical properties of the oils and the position of FAs in the TAG molecule affects the digestion; absorption and metabolism; and physical and technological properties of TAGs. Fixed oils from the fruits of dill (Anethum graveolens), caraway (Carum carvi), cumin (Cuminum cyminum), coriander (Coriandrum sativum), anise (Pimpinella anisum), carrot (Daucus carota), celery (Apium graveolens), fennel (Foeniculum vulgare), and Khella (Ammi visnaga), all from the Apiaceae family, were extracted at room temperature in chloroform/methanol (2:1 v/v) using percolators. Crude lipids were fractionated by solid phase extraction to separate neutral triacylglycerols (TAGs) from other lipids components. Neutral TAGs were subjected to transesterification process to convert them to their corresponding fatty acids methyl esters (FAMES) using 1% boron trifluoride (BF3) in methanol. FAMES were analyzed by gas chromatography-quadrupole time of flight (GC-QTOF) mass spectrometry. Triglycerides were analyzed using high performance liquid chromatography-quadrupole time of flight (LC-QTOF) mass spectrometry. Petroselinic acid was the major fatty acid in all samples ranging from 57% of the total fatty acids in caraway up to 82% in fennel. All samples contained palmitic (16:0), palmitoleic (C16:1n-9), stearic (C18:0), petroselinic (C18:1n-12), linoleic (C18:2n-6), linolinic (18:3n-3), and arachidic (C20:0) acids. TAG were analyzed using LC-QTOF for accurate mass identification and mass spectrometry/mass spectrometry (MS/MS) techniques for regiospesific elucidation of the identified TAGs. Five major TAGs were detected in all samples but with different relative concentrations in all of the tested samples. Several other TAGs were detected as minor components and were present in some samples and absence in the others. Regiospecific analysis showed a non-random fatty acids distribution. Petroselinic acid was predominantly located at the sn-1 and sn-3 positions.
DFT Studies on the Stereoselectivity of α-Silyloxy Diazoalkane Cycloadditions
Authors: O’Connor Matthew , Liu Huaqing , Lee Daesung , Zhou Tao , Xia Yuanzhi
Published: December 2015

The intramolecular [3+2] cycloaddition (32CA) of alkene-tethered α-silyloxydiazoalkanes provides variable stereoselectivity in generating bicyclic pyrazolines where the silyloxy group is either syn or anti to the newly formed pyrazoline ring. To elucidate the origin of the stereoselectivity, density functional theory (DFT) calculations were carried out for the energy of each transition state structure (TSs) and product. Steric effects were identified as the major determining factors in the diastereoselectivity of the 32CA reaction with regards to substrate structure (cyclic or acyclic α-silyloxydiazoalkanes).
Copper-2 Ingestion, Plus Increased Meat Eating Leading to Increased Copper Absorption, Are Major Factors Behind the Current Epidemic of Alzheimer’s Disease
Authors: Brewer George
Published: December 2015

It has become clear that copper toxicity is playing a major role in Alzheimer’s disease; but why is the brain copper toxicity with cognition loss in Alzheimer’s disease so much different clinically than brain copper toxicity in Wilson’s disease, which results in a movement disorder? Furthermore, why is the inorganic copper of supplement pills and in drinking water so much more damaging to cognition than the organic copper in food? A recent paper, which shows that almost all food copper is copper-1, that is the copper-2 of foods reverts to the reduced copper-1 form at death or harvest, gives new insight into these questions. The body has an intestinal transport system for copper-1, Ctr1, which channels copper-1 through the liver and into safe channels. Ctr1 cannot absorb copper-2, and some copper-2 bypasses the liver, ends up in the blood quickly, and is toxic to cognition. Humans evolved to handle copper-1 safely, but not copper-2. Alzheimer’s is at least in part, a copper-2 toxicity disease, while Wilson’s is a general copper overload disease. In this review, we will show that the epidemiology of the Alzheimer’s epidemic occurring in developed, but not undeveloped countries, fits with the epidemiology of exposure to copper-2 ingestion leached from copper plumbing and from copper supplement pill ingestion. Increased meat eating in developed countries is also a factor, because it increases copper absorption, and thus over all copper exposure.
Protective Effect of Vanillic Acid against Hyperinsulinemia, Hyperglycemia and Hyperlipidemia via Alleviating Hepatic Insulin Resistance and Inflammation in High-Fat Diet (HFD)-Fed Rats
Authors: Chang Wen-Chang , Wu James , Chen Chen-Wen , Kuo Po-Ling , Chien Hsu-Min , Wang Yuh-Tai , Shen Szu-Chuan
Published: December 2015

Excess free fatty acid accumulation from abnormal lipid metabolism results in the insulin resistance in peripheral cells, subsequently causing hyperinsulinemia, hyperglycemia and/or hyperlipidemia in diabetes mellitus (DM) patients. Herein, we investigated the effect of phenolic acids on glucose uptake in an insulin-resistant cell-culture model and on hepatic insulin resistance and inflammation in rats fed a high-fat diet (HFD). The results show that vanillic acid (VA) demonstrated the highest glucose uptake ability among all tested phenolic acids in insulin-resistant FL83B mouse hepatocytes. Furthermore, rats fed HFD for 16 weeks were orally administered with VA daily (30 mg/kg body weight) at weeks 13–16. The results show that levels of serum insulin, glucose, triglyceride, and free fatty acid were significantly decreased in VA-treated HFD rats (p < 0.05), indicating the protective effects of VA against hyperinsulinemia, hyperglycemia and hyperlipidemia in HFD rats. Moreover, VA significantly reduced values of area under the curve for glucose (AUCglucose) in oral glucose tolerance test and homeostasis model assessment-insulin resistance (HOMA-IR) index, suggesting the improving effect on glucose tolerance and insulin resistance in HFD rats. The Western blot analysis revealed that VA significantly up-regulated expression of hepatic insulin-signaling and lipid metabolism-related protein, including insulin receptor, phosphatidylinositol-3 kinase, glucose transporter 2, and phosphorylated acetyl CoA carboxylase in HFD rats. VA also significantly down-regulated hepatic inflammation-related proteins, including cyclooxygenase-2 and monocyte chemoattractant protein-1 expressions in HFD rats. These results indicate that VA might ameliorate insulin resistance via improving hepatic insulin signaling and alleviating inflammation pathways in HFD rats. These findings also suggest the potential of VA in preventing the progression of DM.
Gliadin-Specific T-Cells Mobilized in the Peripheral Blood of Coeliac Patients by Short Oral Gluten Challenge: Clinical Applications
Authors: Picascia Stefania , Mandile Roberta , Auricchio Renata , Troncone Riccardo , Gianfrani Carmen
Published: December 2015

Celiac disease (CD) is a common lifelong food intolerance triggered by dietary gluten affecting 1% of the general population. Gliadin-specific T-cell lines and T-cell clones obtained from intestinal biopsies have provided great support in the investigation of immuno-pathogenesis of CD. In the early 2000 a new in vivo, less invasive, approach was established aimed to evaluate the adaptive gliadin-specific T-cell response in peripheral blood of celiac patients on a gluten free diet. In fact, it has been demonstrated that three days of ingestion of wheat-containing food induces the mobilization of memory T lymphocytes reactive against gliadin from gut-associated lymphoid tissue into peripheral blood of CD patients. Such antigen-specific T-cells releasing interferon-γ can be transiently detected by using the enzyme-linked immunospot (ELISPOT) assays or by flow cytometry tetramer technology. This paper discusses the suitability of this in vivo tool to investigate the repertoire of gluten pathogenic peptides, to support CD diagnosis, and to assess the efficacy of novel therapeutic strategies. A systematic review of all potential applications of short oral gluten challenge is provided.
Seismic Behavior of Substandard RC Columns Retrofitted with Embedded Aramid Fiber Reinforced Polymer (AFRP) Reinforcement
Authors: Seyhan Engin , Goksu Caglar , Uzunhasanoglu Ahmet , Ilki Alper
Published: December 2015

Many existing reinforced concrete structures were constructed with substandard characteristics. Low quality concrete, poor transverse reinforcement details and insufficient flexural strength are among the most common deficiencies. While substandard structures are in need of retrofitting, particularly in seismic areas, problems such as high costs and disturbance to occupants are major obstacles for retrofit interventions. Fiber reinforced polymers can provide feasible retrofit solutions with minimum disturbance to occupants. In this study, the basic aim is to investigate the flexural seismic performance of substandard reinforced concrete columns retrofitted with embedded longitudinal fiber reinforced polymer reinforcement without increasing the original dimensions of the columns. In the experimental study, the reference and retrofitted columns were tested under constant vertical and reversed cyclic lateral loads. Three different connection methods of aramid fiber reinforced polymer reinforcement to the footing were investigated experimentally. A significant enhancement was obtained in lateral flexural strength through the proposed retrofitting method. Furthermore, it was observed that the cyclic lateral drift capacities of the retrofitted columns were as high as 3%, which can be deemed as quite satisfactory against seismic actions. The comparison of the experimental data with analytical calculations revealed that a conventional design approach assuming composite action between concrete and fiber reinforced polymer reinforcement can be used for flexural retrofit design. Experimental results also demonstrated that strain limit for longitudinal fiber reinforced polymer (FRP) reinforcement should be remarkably lower in case of reversed cyclic loading conditions.
Synthesis of ABA Tri-Block Co-Polymer Magnetopolymersomes via Electroporation for Potential Medical Application
Authors: Bain Jennifer , Berry Matthew , Dirks Catherine , Staniland Sarah
Published: December 2015

The ABA tri-block copolymer poly(2-methyloxazoline)–poly(dimethylsiloxane)–poly(2-methyloxazoline) (PMOXA–PDMS–PMOXA) is known for its capacity to mimic a bilayer membrane in that it is able to form vesicular polymersome structures. For this reason, it is the subject of extensive research and enables the development of more robust, adaptable and biocompatible alternatives to natural liposomes for biomedical applications. However, the poor solubility of this polymer renders published methods for forming vesicles unreproducible, hindering research and development of these polymersomes. Here we present an adapted, simpler method for the production of PMOXA–PDMS–PMOXA polymersomes of a narrow polydispersity (45 ± 5.8 nm), via slow addition of aqueous solution to a new solvent/polymer mixture. We then magnetically functionalise these polymersomes to form magnetopolymersomes via in situ precipitation of iron-oxide magnetic nanoparticles (MNPs) within the PMOXA–PDMS–PMOXA polymersome core and membrane. This is achieved using electroporation to open pores within the membrane and to activate the formation of MNPs. The thick PMOXA–PDMS–PMOXA membrane is well known to be relatively non-permeable when compared to more commonly used di-block polymer membranes due a distinct difference in both size and chemistry and therefore very difficult to penetrate using standard biological methods. This paper presents for the first time the application of electroporation to an ABA tri-block polymersome membrane (PMOXA–PDMS–PMOXA) for intravesicular in situ precipitation of uniform MNPs (2.6 ± 0.5 nm). The electroporation process facilitates the transport of MNP reactants across the membrane yielding in situ precipitation of MNPs. Further to differences in length and chemistry, a tri-block polymersome membrane structure differs from a natural lipid or di-block polymer membrane and as such the application and effects of electroporation on this type of polymersome is entirely novel. A mechanism is hypothesised to explain the final structure and composition of these biomedically applicable tri-block magnetopolymersomes.
Addendum: Li, Y.; Jing, J.; Jin, H.; Qiao, W. Building Keypoint Mappings on Multispectral Images by a Cascade of Classifiers with a Resurrection Mechanism. Sensors 2015, 15, 11769–11786
Authors: Li Yong , Jing Jing , Jin Hongbin , Qiao Wei
Published: December 2015

The authors wish to update the Acknowledgments in their paper published in Sensors [1], [...]
A Primer on Autonomous Aerial Vehicle Design
Authors: Coppejans Hugo , Myburgh Herman
Published: December 2015

There is a large amount of research currently being done on autonomous micro-aerial vehicles (MAV), such as quadrotor helicopters or quadcopters. The ability to create a working autonomous MAV depends mainly on integrating a simultaneous localization and mapping (SLAM) solution with the rest of the system. This paper provides an introduction for creating an autonomous MAV for enclosed environments, aimed at students and professionals alike. The standard autonomous system and MAV automation are discussed, while we focus on the core concepts of SLAM systems and trajectory planning algorithms. The advantages and disadvantages of using remote processing are evaluated, and recommendations are made regarding the viability of on-board processing. Recommendations are made regarding best practices to serve as a guideline for aspirant MAV designers.
Improving GLONASS Precise Orbit Determination through Data Connection
Authors: Liu Yang , Ge Maorong , Shi Chuang , Lou Yidong , Wickert Jens , Schuh Harald
Published: December 2015

In order to improve the precision of GLONASS orbits, this paper presents a method to connect the data segments of a single station-satellite pair to increase the observation continuity and, consequently, the strength of the precise orbit determination (POD) solution. In this method, for each GLONASS station-satellite pair, the wide-lane ambiguities derived from the Melbourne–Wübbena combination are statistically tested and corrected for phase integer offsets and then the same is carried out for the narrow-lane ambiguities calculated from the POD solution. An experimental validation was carried out using one-month GNSS data of a global network with 175 IGS stations. The result shows that, on average, 27.1% of the GLONASS station-satellite pairs with multiple data segments could be connected to a single long observation arc and, thus, only one ambiguity parameter was estimated. Using the connected data, the GLONASS orbit overlapping RMS at the day boundaries could be reduced by 19.2% in ideal cases with an averaged reduction of about 6.3%.
Google TV or Apple TV?—The Reasons for Smart TV Failure and a User-Centered Strategy for the Success of Smart TV
Authors: Shin Jungwoo , Park Yuri , Lee Daeho
Published: December 2015

Traditional television (TV) has evolved into smart TV in terms of both hardware and software. However, compared with smart phones and tablet PCs, which are huge successes in the market, smart TV has grown more slowly than the market expected and has not really changed the TV market. In this study, we investigate reasons for the failure of smart TV from consumer perspectives. We use conjoint analysis to collect stated preference data from consumers. Our analysis consists of two parts: analyzing consumer preferences for six attributes of smart TVs and examining the effects of socio-demographic and behavioral information on purchase intention for a smart TV. Based on the estimation results from the first part, we find that consumers set a higher value on the traditional characteristics of TV than on the functions of smart TV. Thus, smart TV does not have key functions to encourage its adoption over traditional TV. From the second part of our analysis, we identify which factor is most important to increase purchase intention for a smart TV. Based on our results, we can suggest the direction of market strategies about how to cross the chasm of smart TV.
A Scorecard Framework Proposal for Improving Software Factories’ Sustainability: A Case Study of a Spanish Firm in the Financial Sector
Authors: Álvarez César , Rodríguez Vicente , Ortega Francisco , Villanueva Joaquín
Published: December 2015

Financial institutions and especially banks have always been at the forefront of innovation in management policies in order to improve their performance, and banking is probably one of the sectors that more effectively measures productivity and efficiency in virtually all aspects of its business. However, there is one area that still fails: the productivity of its software development projects. For years banking institutions have chosen to outsource their software projects using software firms created by them for this purpose, but up until a few years ago, the deadline for the delivery of the projects was more important than the efficiency with which they were developed. The last economic crisis has forced financial institutions to review and improve the software development efficiency related to their software factories to achieve a sustainable and feasible model. The sustainability of these software factories can be achieved by improving their strategic management, and the Balanced Scorecard (BSC) framework can be very useful in order to obtain this. Based on the concepts and practices of the BSC, this paper proposes a specific model to establish this kind of software factory as a way of improving their sustainability and applies it to a large Spanish firm specializing in financial sector software. We have included a preliminary validation plan as well as the first monitoring results. The adoption is still very recent and more data are needed to measure all the perspectives so no definitive conclusions can be drawn.
When and Why Did Brains Break Symmetry?
Authors: Rogers Lesley , Vallortigara Giorgio
Published: December 2015

Asymmetry of brain function is known to be widespread amongst vertebrates, and it seems to have appeared very early in their evolution. In fact, recent evidence of functional asymmetry in invertebrates suggests that even small brains benefit from the allocation of different functions to the left and right sides. This paper discusses the differing functions of the left and right sides of the brain, including the roles of the left and right antennae of bees (several species) in both short- and long-term recall of olfactory memories and in social behaviour. It considers the likely advantages of functional asymmetry in small and large brains and whether functional asymmetry in vertebrates and invertebrates is analogous or homologous. Neural or cognitive capacity can be enhanced both by the evolution of a larger brain and by lateralization of brain function: a possible reason why both processes occur side-by-side is offered.
The Role of Rho GTPases in Toxicity of Clostridium difficile Toxins
Authors: Chen Shuyi , Sun Chunli , Wang Haiying , Wang Jufang
Published: December 2015

Clostridium difficile (C. difficile) is the main cause of antibiotic-associated diarrhea prevailing in hospital settings. In the past decade, the morbidity and mortality of C. difficile infection (CDI) has increased significantly due to the emergence of hypervirulent strains. Toxin A (TcdA) and toxin B (TcdB), the two exotoxins of C. difficile, are the major virulence factors of CDI. The common mode of action of TcdA and TcdB is elicited by specific glucosylation of Rho-GTPase proteins in the host cytosol using UDP-glucose as a co-substrate, resulting in the inactivation of Rho proteins. Rho proteins are the key members in many biological processes and signaling pathways, inactivation of which leads to cytopathic and cytotoxic effects and immune responses of the host cells. It is supposed that Rho GTPases play an important role in the toxicity of C. difficile toxins. This review focuses on recent progresses in the understanding of functional consequences of Rho GTPases glucosylation induced by C. difficile toxins and the role of Rho GTPases in the toxicity of TcdA and TcdB.
Mass Spectrometry-Based Method of Detecting and Distinguishing Type 1 and Type 2 Shiga-Like Toxins in Human Serum
Authors: Silva Christopher , Erickson-Beltran Melissa , Skinner Craig , Patfield Stephanie , He Xiaohua
Published: December 2015

Shiga-like toxins (verotoxins) are responsible for the virulence associated with a variety of foodborne bacterial pathogens. Direct detection of toxins requires a specific and sensitive technique. In this study, we describe a mass spectrometry-based method of analyzing the tryptic decapeptides derived from the non-toxic B subunits. A gene encoding a single protein that yields a set of relevant peptides upon digestion with trypsin was designed. The 15N-labeled protein was prepared by growing the expressing bacteria in minimal medium supplemented with 15NH4Cl. Trypsin digestion of the 15N-labeled protein yields a set of 15N-labeled peptides for use as internal standards to identify and quantify Shiga or Shiga-like toxins. We determined that this approach can be used to detect, quantify and distinguish among the known Shiga toxins (Stx) and Shiga-like toxins (Stx1 and Stx2) in the low attomole range (per injection) in complex media, including human serum. Furthermore, Stx1a could be detected and distinguished from the newly identified Stx1e in complex media. As new Shiga-like toxins are identified, this approach can be readily modified to detect them. Since intact toxins are digested with trypsin prior to analysis, the handling of intact Shiga toxins is minimized. The analysis can be accomplished within 5 h.