bluuebunny/crossref_metadata_2025 huggingface dataset:
A multiple sclerosis lifestyle behavior online course: Qualitative analysis of participants' motivations, expectations and experiences
Authors: Neate Sandra L. , Bevens William , Jelinek Pia L. , Gray Kathleen M. , Weiland T. J. , Nag Nupur , Simpson-Yap Steve , Jelinek George A. , Yu M. , Reece Jeanette C.
Published: December 2022

Background

Modification of lifestyle-related risk factors for multiple sclerosis (MS) has been associated with improved health outcomes when compared with standard medical management alone. Based on an existing lifestyle modification program offered as a residential workshop, the MS Online Course (MSOC) was developed to translate the workshop into an online intervention. We performed a pilot randomized controlled trial (RCT), to assess the feasibility concepts of accessibility, learnability and desirability through quantitative and qualitative analyzes. In the present study, we performed additional qualitative analyzes to explore participants' motivations, expectations, and experiences of the MSOC. This study aims to complement prior feasibility analyzes and inform recruitment strategies and course content redevelopment so that its effectiveness may be assessed by examining behavior change and health outcomes in a future larger RCT.

Methods

Participants were recruited via online advertisements and randomized to either: the standard care course, containing material sourced from public facing MS websites; or the intervention course, based on an evidence-based lifestyle modification program for people with MS. Course completers were invited to participate in semi-structured interviews. Within a qualitative paradigm, reflexive thematic analysis of interviews was undertaken.

Results

Of 31 eligible participants, 17 completed the MSOC and 14 agreed to be interviewed. Four themes were identified in this analysis: (1) “Wanting to help others” (helping through volunteering, contributing to knowledge base, spreading the word; (2) “Seeking knowledge” (confirmation of existing knowledge; obtaining new knowledge, relevant, credible information); (3) “Doing what I can to help myself” (understanding lifestyle modification, changing my lifestyle, remaining well); and (4) “Changing attitudes” (finding positivity, feeling more confident and in control).

Conclusions

Participants were motivated to help others through research, help themselves by improving knowledge and to find ways to better manage their MS. Expectations included obtaining credible, reliable information, to substantiate existing knowledge, and to further understand lifestyle modification. Participants' experiences included confirmation of and obtaining new knowledge, and early implementation of modified lifestyle behaviors. These insights surrounding participants' motivations, expectations and experiences will assist in recruitment strategies, course redevelopment and outcome measures for the future RCT to examine the effectiveness of the MSOC.
Chronic kidney disease and its association with cataracts–A cross-sectional and longitudinal study
Authors: Huang Chun-Yen , Lee Jia-In , Chang Chia-Wen , Liu Yao-Hua , Huang Shu-Pin , Chen Szu-Chia , Geng Jiun-Hung
Published: December 2022

Introduction

We aim to explore the association between chronic kidney disease (CKD) and cataracts.

Methods

A total of 121,380 participants with adequate information collected from 29 community-based recruitment centers since 2008 were analyzed. The association between CKD and self-reported diagnosed cataracts was examined in a cross-sectional cohort and was validated in a longitudinal cohort of 25,263 participants without cataracts at baseline.

Results and discussion

Of all participants, cataracts occurred in 503/1,947 (26%) and 10,464/119,433 (9%) subjects in the CKD and non-CKD groups, respectively. Multivariate logistic regression showed that CKD was significantly associated with a higher prevalence of self-reported diagnosed cataracts. In the validation cohort, a higher incidence of cataracts was also noted in the CKD group (65/317, 21%) compared to the non-CKD group (1,964/24,252, 8%) during a mean 47-month follow-up. After adjusting for confounders, subjects with CKD had a 1.498-fold higher risk of incident cataracts than those without CKD (95% confidence interval = 1.114 to 2.013, p value = 0.007). We found that CKD was associated with a higher prevalence of cataracts as well as incident cataracts, which suggests CKD patients and their primary physicians should be aware of this disease and can provide a clue for further exploration of the possible mechanisms and treatments.
High relatedness of bioinformatic data and realistic experimental works on the potentials of Fasciola hepatica and F. gigantica cathepsin L1 as a diagnostic and vaccine antigen
Authors: Fereig Ragab M. , Metwally Samy , El-Alfy El-Sayed , Abdelbaky Hanan H. , Shanab Obeid , Omar Mosaab A. , Alsayeqh Abdullah F.
Published: December 2022

Introduction

Fascioliasis is a parasitic foodborne disease caused by the liver flukes, Fasciola hepatica and F. gigantica. Such parasites cause serious illness in numerous domestic animals and also in humans. Following infection, the parasite secretes a variety of molecules that immediately interact with the host immunity to establish successful infection. These molecules include cathepsin L peptidase 1 (CatL1); the highly investigated diagnostic and vaccine antigens using various animal models. However, a few studies have analyzed the potentials of FhCatL1 as a diagnostic or vaccine antigen using bioinformatic tools and much less for FgCatL1. The present study provides inclusive and exclusive information on the physico-chemical, antigenic and immunogenic properties of F. hepatica cathepsin L1 (FhCatL1) protein using multiple bioinformatic analysis tools and several online web servers. Also, the validation of our employed available online servers was conducted against a huge collection of previously published studies focusing on the properties of FhCatL1as a diagnostic and vaccine antigen.

Methods

For this purpose, the secondary, tertiary, and quaternary structure of FhCatL1 protein were also predicted and analyzed using the SWISS-MODEL server. Validation of the modeled structures was performed by Ramachandran plots. The antigenic epitopes of the protein were predicted by IEDB server.

Results and discussion

Our findings revealed the low similarity of FhCatL1 with mammalian CatL1, lacking signal peptides or transmembrane domain, and the presence of 33 phosphorylation sites. Also, the containment of FhCatL1 for many topological, physico-chemical, immunological properties that favored its function of solubility and interaction with the immune components were reported. In addition, the earlier worldwide reports documented the high efficacy of FhCatL1 as a diagnostic and vaccine antigen in different animals. Altogether, FhCatL1 is considered an excellent candidate for using in commercialized diagnostic assays or vaccine products against fascioliasis in different animal species. Our assessment also included FgCatL1 and reported very similar findings and outputs to those of FhCatL1.
Assessing COVID-19-related health literacy and associated factors among school teachers in Hong Kong, China
Authors: Lau Sam S. S. , Shum Eric N. Y. , Man Jackie O. T. , Cheung Ethan T. H. , Amoah Padmore Adusei , Leung Angela Y. M. , Dadaczynski Kevin , Okan Orkan
Published: December 2022

Objectives

The coronavirus disease 2019 (COVID-19) pandemic developed rapidly, with changing guidelines, misinformation, inaccurate health information and rumors. This situation has highlighted the importance of health literacy, especially among educators. The aims of this study were (i) to assess COVID-19-specific health literacy among school teachers in Hong Kong and (ii) to examine its association with demographic factors, self-endangering work behaviors (i.e., work intensification, work extensification and work quality reduction), secondary burnout symptoms (i.e., exhaustion related to work and psychosomatic complaints), the level of knowledge of COVID-19- or pandemic-related information and the level of confusion about COVID-19-related information.

Methods

A self-report survey was administered to 366 Hong Kong school teachers from April 2021 to February 2022. COVID-19-specific health literacy was measured using the HLS-COVID-Q22 instrument. Other instruments, including self-endangering work behavior scales (i.e., extensification of work, intensification of work and work quality reduction) and two dimensions of the Burnout Assessment Tool (i.e., psychosomatic complaints and exhaustion) were also used for assessment. Data were analyzed using an independent samples Student's t-test, analysis of variance, correlation analysis and adjusted multilinear regression models.

Results

The results showed that 50.8% of school teachers had sufficient health literacy, 38.3% had problematic health literacy and 10.9% had inadequate health literacy. The HLS-COVID score did not vary by sex, but varied according to the type of school, the number of working hours per week and the number of students attending the school. Teachers with sufficient health literacy scored significantly lower for two types of self-endangering work behavior–intensification of work (p = 0.003) and work quality reduction (p = 0.007)—than those with insufficient health literacy. After excluding those who had already been vaccinated, respondents with sufficient health literacy felt more positive about COVID-19 vaccination than those with insufficient health literacy (t[180] = 4.168, p < 0.001). In addition, teachers with sufficient health literacy felt more informed (p < 0.001) and less confused (p < 0.001) about COVID-19-related information than those with insufficient health literacy. Multiple linear regression analysis revealed that age (β = 0.14, p = 0.011) and the number of teaching hours per week (β = −0.206, p < 0.001) were significant predictors of the HLS-COVID score.

Conclusions

The findings of this study may serve as a guide for addressing health literacy gaps among school teachers.
Designing for the post-pandemic era: Trends, focuses, and strategies learned from architectural competitions based on a text analysis
Authors: Han Pei , Wang Lingju , Song Yufei , Zheng Xi
Published: December 2022

The COVID-19 pandemic has made the built environment an important source of prevention and control, architects and scholars have thus been seeking countermeasures since the beginning of the outbreak. As design and construction cycles are long, only a few completed cases and evidence-based studies are available for reference. However, massive architectural competition works have emerged, which always been the soil for discussion and practice of cutting-edge design issues. These contain a vast number of ideas for solutions from various design dimensions—including cities, buildings, and facilities—and provide a great deal of materials worth analyzing and summarizing. Therefore, the exploration of competitions will provide us with public health intervention directions, strategies and a rethinking of the built environment. Using a text-mining approach, we analyzed 558 winning entries in architectural competitions related to the pandemic response, exploring specific issues, populations involved, coping strategies, and trends that emerged as the pandemic evolved. Our results show that the strategies proposed can be grouped into 17 keywords, with modularization being the most frequent strategy and related strategies like rapid assembly, flexible space, etc. are also took a significant percentage of the use. Further, we explored the technical orientation, year, territory, target groups, and target problems of the works which lead to a series of cross-comparison relationships. The results indicate that indirect impacts caused by the pandemic gained more attention and flexible Solutions were used more often highlighted the consensus when adapting to the uncertainties. The focus on the spiritual dimension is increasing year by year reflected the spiritual influences were gaining traction and the indirect impacts gradually showed up over time. The research will provide a strategy reference for the design response to the pandemic, as well as help understand the influence and significance of social factors behind the divergence of issue focuses and strategic tendency in different regions and times.
Urbanization and human security in post-colonial Africa
Authors: Eyita-Okon Ekeminiabasi
Published: December 2022

Urbanization has been unprecedented in post-colonial Africa. While cities and towns (urban areas) are crucial to bolstering economic activities, they are equally perceived as the scene for social and economic problems. This in turn exacerbates urban human insecurity. The paper draws on qualitative research method in exploring the challenges and opportunities that undermine or facilitate achieving sustainable and inclusive cities in post-colonial Africa. Particularly, the role of the African Union through Agenda 2063 in materializing sustainable and efficient cities, against the backdrop of climate change, in post-colonial Africa. The study present statistics on urbanization and human security trends in the region such as the rate of urbanization, quality of life in informal settlements and slums, the share of urban population living in slums, and a correlation matrix on the quality of human development in slums. The study finds that while varied strides have been taken across the African continent on a national level, the problem of coordination, structural/systemic violence, prevalence of external vision for Africa's development, and gaps in policy implementation pose a threat to adequately addressing the perils of urbanization on human security in post-colonial Africa. Irrespective, Agenda 2063 offers the continent the opportunity to prioritize urban human insecurity issues as an emerging non-conventional security threat requiring securitization. Also, it offers an opportunity to re-center people at the heart of policy decisions while creating incentives for member states to meet the aspirations, goals, and targets set out in Agenda 2063, as it pertains to sustainable and inclusive cities in post-colonial Africa.
The perception of injury risk and prevention among football players: A systematic review
Authors: Cardoso-Marinho Beatriz , Barbosa Ana , Bolling Caroline , Marques José Pedro , Figueiredo Pedro , Brito João
Published: December 2022

Football is associated with a certain risk of injury, leading to short- and long-term health consequences. However, the perception of football players about injury risk and prevention strategies is poorly documented. The present article reviewed the literature about perceptions, beliefs, attitudes and knowledge toward injury risk and prevention strategies in football players. An electronic search was performed in PubMed, Scopus, Web of Science, and APA PsychINFO until July 2022. Studies were eligible if they included the perceptions, beliefs, attitudes, and knowledge about injury risk and prevention in football players from any competitive level. The risk of bias was assessed in included studies using the Joanna Briggs Institute critical appraisal checklist. A total of 14 studies were included. Most football players agreed that their risk of injury is high and prevention strategies are important, however they do not intend to use some of these strategies. The most frequent perceived injury risk factors were low muscle strength, lack of physical fitness, fatigue, excessive training and type and condition of surfaces. The most frequent perceived injury prevention factors were warm-up, workload monitoring and strength and conditioning training. It is essential to acknowledge perceived injury risk factors, as well as a better understanding of how coaching and medical departments' perceptions match with players' perceptions, and a modification in the perceptions of the several stakeholders at different levels of action.
Cropping system intensification for smallholder farmers in coastal zone of West Bengal, India: A socio-economic evaluation
Authors: Mandal Subhasis , Sarangi Sukanta Kumar , Mainuddin M. , Mahanta K. K. , Mandal Uttam Kumar , Burman D. , Digar S. , Sharma P. C. , Maji B.
Published: December 2022

Introduction

It is estimated that five out of six farms in the world are operating less than two hectares of land, called smallholder farmers, and they are producing over one third of the global food. Cropping system intensification research and interventions at farmers' fields could be one of the ways to improve the prevailing cropping systems. Understanding socio-economic issues are important for the successful implementation of improved or new cropping systems and for increasing farmers' income in the coastal zone of the Ganges delta. A socio-economic evaluation study was carried out to understand how far the suggested cropping options were feasible to smallholder farmers in the coastal zone; quantify the benefits due to the adoption of new cropping systems; how far those options were socio-economically suitable for the targeted smallholder farmers; and to identify the key factors that might be affecting the out-scaling of the evolved options to a larger group of farmers.

Methods

Baseline and endline surveys were conducted with 90 farmers before and after the demonstration of various cropping systems at farmers' fields. Techno-economic suitability of new crops and management options were evaluated through accounting benefits of adoption and identifying various constraints in adoption. Behavioral analysis was carried out to identify factors affecting large-scale adoption of the new/improved cropping systems evolved.

Results and discussion

The socio-economic survey quantified the increase in cropping intensity higher than the baseline level (123–142%) and reduced the rabi (winter/dry) season fallow area by 30–35%. The study identified farmers' preferred interventions were low-cost drip irrigation and mulching, zero-tillage (ZT) potato with straw mulching, improving soil quality with lime and green manuring, and vegetable-based cropping systems interventions. Although the economics of the evolved cropping systems were favorable, however, availability of freshwater stored in ponds/canals, and income from on and off-farm were the most important factors determining the adoption of new systems on a larger scale.
An Improved Dynamic Model and Matrix Displacement Model for Distributed-Compliance Bridge-Type Amplification Mechanism
Authors: Li Peixing , Zhu Helei , Lai Leijie
Published: December 2022

This paper establishes a matrix displacement model and an improved dynamic model for the static and dynamic performances analysis for a kind of bridge-type displacement amplification mechanism with distributed-compliance, which has better performances than traditional lumped-compliance bridge-type mechanisms. In the matrix displacement model, the stiffness matrix for two rigid bodies connected by flexures is first obtained by regarding the displacements and the forces on two mass centers of the rigid bodies as the node displacements and node forces. By extending and superimposing each elemental stiffness matrix, the global stiffness matrix for the flexure mechanism can be obtained to calculate the displacement amplification ratio and input stiffness of the bridge-type mechanism. In the improved dynamic model, in order to establish the Lagrangian dynamic model more accurately, the deflectional, axial, and rotational velocities of any point on the beam flexure are calculated by solving the derivatives of the deformation curves of beam flexures versus time to obtain the expression of the kinetic energy in the vibrating beams. On this basis, the three-degree-of-freedom vibration differential equation for the bridge-type mechanism is established by using the Lagrange method, and the natural frequency in the working direction is obtained accurately. The presented models are compared with the finite element analysis, and experiments for two case studies of the bridge-type distributed-compliance mechanism are presented. The comparisons results demonstrate the high prediction accuracy of the improved dynamic model.
Flight Time Optimization and Modeling of a Hybrid Gasoline–Electric Multirotor Drone: An Experimental Study
Authors: Jarrah Khaled , Alali Yazen , Lalko Alyssa , Rawashdeh Osamah
Published: December 2022

Drones have evolved rapidly over the decades, but the limited flight time inhibits multirotor drones from performing long-duration tasks. Batteries that power drones are considered an inadequate power source due to their low energy density. As gasoline is an energy-dense source, combining an electric propulsion system with gasoline engines should be considered. This paper proposes a novel hybrid multirotor drone design using two gasoline engines to provide the majority of the lift force and four electric motors to stabilize the drone. These propulsion systems have been characterized and optimized to exploit their respective advantages, which reduce the total energy consumption rate and increase flight time. Simulation and experimental results show that the hybrid gas–electric multirotor drone can achieve more than three times the flight time of the fully electric drone.
Assessing the Sustainability of Liquid Hydrogen for Future Hypersonic Aerospace Flight
Authors: Mohammad Abdalrahman Khaled , Sumeray Charles , Richmond Maximilian , Hinshelwood Justin , Ghosh Aritra
Published: December 2022

This study explored the applications of liquid hydrogen (LH2) in aerospace projects, followed by an investigation into the efficiency of ramjets, scramjets, and turbojets for hypersonic flight and the impact of grey, blue, and green hydrogen as an alternative to JP-7 and JP-8 (kerosene fuel). The advantage of LH2 as a propellant in the space sector has emerged from the relatively high energy density of hydrogen per unit volume, enabling it to store more energy compared to conventional fuels. Hydrogen also has the potential to decarbonise space flight as combustion of LH2 fuel produces zero carbon emissions. However, hydrogen is commonly found in hydrocarbons and water and thus it needs to be extracted from these molecular compounds before use. Only by considering the entire lifecycle of LH2 including the production phase can its sustainability be understood. The results of this study compared the predicted Life Cycle Assessment (LCA) emissions of the production of LH2 using grey, blue, and green hydrogen for 2030 with conventional fuel (JP-7 and JP-8) and revealed that the total carbon emissions over the lifecycle of LH2 were greater than kerosene-derived fuels.
Stability Study of Time Lag Disturbance in an Automatic Tractor Steering System Based on Sliding Mode Predictive Control
Authors: Miao Hequan , Diao Peisong , Yao Wenyan , Li Shaochuan , Wang Wenjun
Published: December 2022

To improve the working accuracy and anti-interference capability of the steering operation of an automatic tractor, this paper investigates the tractor steering system. In response to the current problems of high steering resistance during tractor field operations and the low service life of the drive shaft of conventional electric steering wheel solutions, an electro-hydraulic coupled power-assisted solution combining EPS and HPS is proposed. The combination of the EPS system’s high control accuracy and sensitive steering operation and the hydraulic power system’s large steering torque greatly reduces the power of the power motor and battery performance requirements, optimizing the power transmission scheme to achieve green and energy-saving purposes. Secondly, the research is focused on the influence of external disturbances on the stability of the steering system during tractor operation, and a combination of model predictive control and sliding mode control is used to study the steering system control strategy. It is finally demonstrated through simulations and experiments that it can compensate for the disturbance of the system control parameters by external disturbances, has the ability of MPC to handle input constraints and maintains the advantages of SMC robustness.
Simulation Study for Overturning and Rollover Characteristics of a Tractor with an Implement on a Hard Surface
Authors: Jang Moon-Kyeong , Hwang Seok-Joon , Nam Ju-Seok
Published: December 2022

The effects of the slope of the ground and the obstacle conditions on the lateral overturning/backward rollover of a tractor with an implement were analyzed through dynamic simulation. The tractor and implement’s 3D simulation model was constructed. As for simulation conditions, four heights and three shapes were set for obstacles, and eight slopes were set for the ground to be traveled by the implemented tractor. Under each condition, the critical speed at which the tractor begins to overturn and roll over was derived, and factors that caused the overturn and rollover were analyzed. As a result of instability types, backward rollover happens when the ground slope is low and lateral overturning happens at a specific slope or higher regardless of the obstacle conditions. In the case of the tractor and implement under study, the tendency changed at a slope of 25°. As the obstacle height increased, overturning and rollover safety decreased. In the case of the obstacle shape, safety was lowest for the rectangular obstacle and highest for the right-side triangular obstacle. The driving safety of the tractor with the implement was lower than that of the tractor with no implement. This appears to be mainly due to the change in the position of the center of gravity caused by the attached implement. The critical speed of the tractor with the implement was 3.26 times lower than that of the tractor with no implement on average. It is judged that the safety of the implemented tractor can be identified by using this study.
Experimental Study of a 4HLB-4 Half-Feed Four-Row Peanut Combine Harvester
Authors: Wang Qinghua , Yu Zhaoyang , Zhang Yanhua , Li Junhao , Peng Baoliang , Wang Bing , Hu Zhichao
Published: December 2022

In order to improve the operational performance of the self-developed 4HLB-4 half-feed four-row peanut combine harvester, single-factor and multifactor field tests were conducted on key operational parameters affecting the quality of harvesting. The response surface methodology was used to study the effects of the forward speed, clamping height, peanut picking roller rotational speed, and cleaning sieve vibrational frequency on the loss rate and impurity rate. A response surface regression model of the loss rate and impurity rate was constructed. A multi-objective integrated optimization was carried out for each factor. The results showed that, in terms of the order of significance, the influence of each factor on the impurity rate was as follows: cleaning sieve vibrational frequency > clamping height > forward speed > peanut picking roller rotational speed. The order of significance of the influence of each factor on the loss rate was as follows: peanut picking roller rotational speed > forward speed > clamping height > cleaning sieve vibrational frequency. The optimal combination of parameters was a forward speed of 0.85 m/s, clamping height of 190 mm, peanut picking roller rotational speed of 550 rpm, and cleaning sieve vibrational frequency of 590 cpm. Under these conditions, the impurity rate of 2.62% and the loss rate of 2.05% were obtained, which effectively reduced the impurity rate and loss rate and met the quality requirements of Chinese peanut mechanized harvesting operations. The results of this study can provide a basis for the improvement and optimization of the 4HLB-4 half-feed four-row peanut combine harvester and the optimization of its operating parameters.
Adsorption of Atrazine by Fe-Mn-Modified Biochar: The Dominant Mechanism of π–π Interaction and Pore Structure
Authors: Liang Yuan , Zhao Ben , Yuan Chuqi
Published: December 2022

It is important to explore efficient materials to alleviate the negative effect of atrazine in soils or water. In this paper, four Fe/Mn-modified biochars were prepared to enhance atrazine removal. The batch adsorption experiment was conducted to explore the adsorption ability of biochar(DBC) and modified biochars (F3M1DBC, F1M3DBC, MnDBC, and FeDBC) on atrazine, and the adsorption mechanism was conducted by XRD, XPS, and FTIR. The modified biochar showed larger specific surface areas and zero-point charge than those of the original biochar. The increased oxygen functional groups (OH, C=C, and C=O) and the formation of Fe3O4, Mn3O4, and FeMnO3 on modified biochar improved atrazine removal. The maximum atrazine adsorption by F3M1DBC was 4.3 times higher than that of DBC. The atrazine adsorption by modified biochar was not pH-dependent, and their removal of atrazine was dominated by adsorption rather than degradation. The desorption rate was 8.61% for F3M1DBC, 15.95% for F1M3DBC, 26.19% for MnDBC, and 29.83% for FeDBC, which were 29.1–79.5% lower than that of DBC, accordingly decreased the environmental risk. XPS and FTIR analysis proved that the adsorption mechanisms of Fe/Mn-modified biochars were mainly attributed to their strong π–π interactions between atrazine and oxygen functional groups, graphitic carbon, and Fe/Mn-oxides on the surface of biochar. In addition, the larger surface area and pore structure of modified biochar contributed to the adsorption and pore filling of atrazine on biochar. In general, the Fe/Mn-modified biochars can be used as effective adsorbents to remove atrazine from soils and waters.
Combined Physio-Biochemical and Transcriptome Analyses Illuminate the Resistance Response of Rice Priming with Decoyinine against Nilaparvata lugens
Authors: Ma Chao , Gui Wei , Zhang Yuanyuan , Shah Amir Zaman , Xu Gang , Yang Guoqing
Published: December 2022

The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a notorious pest in rice production. The microbial secondary metabolite, decoyinine (DCY), is produced by Streptomyces hygroscopicus. Recent studies found that seed priming with DCY could enhance rice resistance to BPH and Laodelphax striatellus; however, the mechanism of enhancing insect resistance in rice remains unclear. Here, an integrated physio-biochemical and transcriptome analysis was performed on rice priming with DCY after BPH infestation. Defense-related enzymes activities such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione S transferase (GST), and polyphenol oxidase (PPO), concentrations of hydrogen peroxide (H2O2), callose, and free amino acids in rice priming with DCY were significantly increased than in control plants after BPH infestation. Concentrations of soluble sugars, sucrose, and glucose were higher in rice treated with DCY than in the control after BPH infestation; however, the concentration of malondialdehyde (MDA) was significantly decreased in rice treated with DCY after BPH infestation. In the transcriptome analysis, GO functional annotation and KEGG pathway analysis were enriched in defense response, transcription factors, secondary metabolites, reactive oxygen species, and cell wall organization and these data also support physio-biochemical results. The qRT-PCR results further verified the differential expressed genes related to DCY-treated rice responding to BPH. Meanwhile, it indicated that DCY might enhance the resistance of rice to BPH by regulating the rice WRKY transcription factor genes. Our results provide a basis for further exploring the molecular mechanism of the defense response of rice priming with DCY against BPH infestation and could provide valuable resources to control insect pests.
Growth, Leaf Pigment Content, and Antioxidant Potential of Ferns Grown in Peat Substrate Amended with Camelina Press Cake
Authors: Pietrak Anna , Łopusiewicz Łukasz , Salachna Piotr
Published: December 2022

Using natural waste as a source of minerals and biostimulants can offer many advantages in the production of plants in containers. The research aimed to evaluate the usefulness of cakes obtained from the production of camelina seed oil (Camelina sativa) in the cultivation of ferns. The greenhouse experiment used ornamental ferns rich in health-promoting compounds: Onoclea sensibilis, Polypodium vulgare, and Polystichum polyblepharum. Plants were planted in pots filled with peat substrate mixed with camelina press cake in the form of pellets at a dose of 1 g/L or 5 g/L. Plants grown in 100% peat substrate constituted the control. It was found that the plants grown in the medium enriched with press cake were higher (P. vulgare and P. polyblepharum), wider (O. sensibilis and P. polyblepharum), and had increased dry weight (O. sensibilis and P. polyblepharum) regardless of the dose of the cake. Moreover, in all species, the addition of press cake significantly increased total chlorophyll content by 23.5–32.5% and carotenoids by 17.7–68.7% compared to the control. The effect of cake on the level of total polyphenols and flavonoids as well as antioxidant activity was unequal and depended mainly on the species and the waste dose. Overall, developing sustainable fern cultivation principles and understanding their nutritional properties could contribute to the broader use of ferns in green spaces, medicine, pharmaceuticals, and cosmetics.
Ecomorphology and Morphological Disparity of Caquetaia Kraussii (Perciformes: Cichlidae) in Colombia
Authors: Hernandez Jordan , Villalobos-Leiva Amado , Bermúdez Adriana , Ahumada-C Daniela , Suazo Manuel J. , Correa Margarita , Díaz Angie , Benítez Hugo A.
Published: December 2022

Understanding the interspecific morphological variability of Caquetaia kraussii (Perciformes: Cichlidae) between different localities in its distribution range is becoming essential, as this species constitutes a valuable resource for the economy and subsistence of the local human communities where it is endemic in Colombia and Venezuela. In order to develop efficient farming and handling plans for this species, a deep understanding of the factors and mechanisms generating morphological variability is crucial. This study analyzes the morphological variability of C. kraussii by using geometric morphometrics in four localities distributed between the Dique and North channels, which are part of the Bolívar department in Colombia. Likewise, the effect of environmental variables such as temperature (T°), dissolved oxygen (OD) and pH on morphological variability was analyzed using a partial least squares approach. The results show that environmental stress has an influence on ~10% of the body shape of C. kraussii, whereas ~90% of the body shape is not directly influenced by environmental parameters, suggesting an effect from stress related to sexual dimorphism. Similarly, the analyses show shape variation among localities, mainly between populations of lotic environments and those of lentic environments. This morphological disparity seems to be subject to environmental and sexual stresses in the different localities.
The Use of Prefemoral Endoscope-Assisted Surgery and Transplastron Coeliotomy in Chelonian Reproductive Disorders
Authors: Hellebuyck Tom , Solanes Vilanova Ferran
Published: December 2022

Throughout the last decades, the increased popularity of the keeping of reptiles has led to a better understanding of the captive needs of a wide variety of species. Although this is reflected by the successful captive reproduction in many of those species, reproductive disorders such as preovulatory follicular stasis, postovulatory dystocia, secondary yolk coelomitis, and prolapse of the oviduct and male copulatory organ are commonly encountered in veterinary practice. In comparison to squamates, chelonians with postovulatory dystocia seem to be more responsive to oxytocin treatment, even in cases of chronic dystocia. There are various conditions, however, that necessitate the use of surgical procedures for the treatment of dystocia and other reproductive disorders in chelonians. Although restrictions may be encountered, the endoscope-assisted prefemoral approach is the least invasive and thus preferred technique instead of the ventral transplastron coeliotomy. The present report describes the diagnostic and surgical approach applied in seven cases of female chelonians with reproductive disorders. The therapeutic efficacy largely relied on the choice of minimally invasive endoscope-assisted surgery versus transplastron coeliotomy that was primarily dictated by the involved species, etiology, and associated pathology.
Effects of Landscape Features on Bird Community in Winter Urban Parks
Authors: Huang Peilin , Zheng Dulai , Yan Yijing , Xu Weizhen , Zhao Yujie , Huang Ziluo , Ding Yinghong , Lin Yuxin , Zhu Zhipeng , Chen Ziru , Fu Weicong
Published: December 2022

Urban parks, as critical components of the urban green space, have practical significance in studying the influence of landscape characteristics on birds. Nine urban parks in Fuzhou, China, were used as study objects to explore the influence of landscape features (patch, landscape, and surrounding environment indices) on bird communities. The results showed that (1) from December 2021 to February 2022, we found a total of 2874 individuals belonging to 61 species of 9 orders, 32 families, which were dominated by the birds of Passeriformes (37 species of 24 families, accounting for 89.91% of the total number of individuals) and resident birds in Fuzhou urban parks (n = 30; 85.46%); (2) The park area, park perimeter, woodland area, grassland area, and the park shape index increased as the distance to the city center increases; (3) Bird diversity responds differently to different landscape features. The total abundance of birds, the abundance of winter migrant birds, and the richness of winter migrant birds increased with the park area. And the park shape index affects positively for the the α-diversity of birds and the abundance of resident birds. Woodland proportion and waterbody shape index affected positively on the richness and α-diversity of resident birds. To promote the diversity of regional birds, it is recommended that the construction and planning of urban parks should enlarge the park area as much as possible, increase the proportion of woodland, and make shorelines more irregular. Our study could serve as a reference for the construction of biodiversity enhancements in core green areas of urban parks.
Growth Characteristics of Long-Nosed Skate Dipturus oxyrinchus (Linnaeus, 1758) Inhabiting the Northeastern Mediterranean Sea
Authors: Başusta Nuri , Ozel Fatih Volkan
Published: December 2022

This study aims to determine the age and growth characteristics of Dipturus oxyrinchus living in the Northeastern Mediterranean Sea and to present data that can provide a comparison with previous studies on the same subject. A total of 255 long-nose skates at a total length of 12.2–93.5 cm and weight of 8.34–3828 g were collected as non-target species from a commercial fishing boat. The male−female ratio was determined as 1:1.27. Using the von Bertalanffy equation and the Gompertz or logistic growth models, the growth parameters of Dipturus oxyrinchus were estimated as L∞ = 154.0, K = 0.064, t0 = −1.622; L∞ = 104.0, K = 0.35, I = 4.99; L∞ = 128.40, K = 0.19, I = 4.39 for all individuals, respectively. Maximum absolute growth was calculated as 9.33 cm at 5–6 years of age. Maximum relative growth at 1–2 years of age was estimated as 36.39%. Both absolute and relative growth were minimal in the 11–12 age group. The highest condition factor value was estimated as 0.416 in the 8-year-old group. As a result, the growth data of long-nose skates were obtained for the first time in the Northeastern Mediterranean Sea.
Ultrasound Guided Surgery as a Refinement Tool in Oncology Research
Authors: Camara Serrano Juan Antonio
Published: December 2022

Refinement is one of the ethical pillars of the use of animals in research. Ultrasonography is currently used in human medicine as a surgical tool for guided biopsies and this idea can be applied to preclinical research thanks to the development of specific instruments. This will eliminate the necessity of a surgical opening for implanting cells in specific organs or taking samples from tissues. The approach for the injection will depend on the target but most of the case is going to be lateral, with the probe in a ventral position and the needle going into from the lateral. This is the situation for the thyroid gland, heart, liver, spleen, kidney, pancreas, uterus, and testicles. Other approaches, such as the dorsal, can be used in the spleen or kidney. The maximum injected volume will depend on the size of the structure. For biopsies, the technical protocol is similar to the injection knowing that in big organs such as the liver, spleen, or kidney we can take several samples moving slightly the needle inside the structure. In all cases, animals must be anesthetized and minimum pain management is required after the intervention.
Distinct Changes in Microbiota-Mediated Intestinal Metabolites and Immune Responses Induced by Different Antibiotics
Authors: Yoon Sunghyun , Lee Giljae , Yu Junsun , Lee Kiuk , Lee Kyeongju , Si Jiyeon , You Hyun Ju , Ko GwangPyo
Published: December 2022

The cocktails of antibiotics are utilized to study the functions of microbiota. There have been studies on the alteration of not only the microbiota composition but also the host’s metabolism or immunity. However, the bacterial species associated with these altered physiologic markers are still unclear. Therefore, we supplied mice with drinking water containing ampicillin (AMP), vancomycin (VAN), neomycin (NEO), or metronidazole (MET) to observe the effect of each antibiotic on helper T cells and inflammation-related gene expression and metabolism, including amino acid metabolism and changes in gut microbiota. We observed major changes in gut microbiota in mice treated with AMP and VAN, respectively, immediately after administration. The abundance of the genera Parabacteroides and Akkermansia increased in the AMP and VAN groups, while Prevotella almost disappeared from both groups. The compositional changes in intestinal metabolites in the AMP and VAN groups were more distinct than those in the NEO and MET groups, which was similar to the microbiome results. In particular, the most distinct changes were observed in amino acid related metabolism in AMP and VAN groups; the amounts of phenylalanine and tyrosine were increased in the AMP group while those were decreased in the VAN group. The changed amounts of intestinal amino acids in each of the AMP and VAN groups were correlated with increases in the abundance of the genera Parabacteroides and Akkermansia in the AMP and VAN groups, respectively. The most distinctive changes in intestinal gene expression were observed in the ileum, especially the expression Th17-related genes such as rorgt, il17a, and il17f, which decreased dramatically in the guts of most of the antibiotic-treated groups. These changes were also associated with a significant decrease in Prevotella in both the AMP and VAN groups. Taken together, these findings indicate that changes in gut microbiota as well as host physiology, including host metabolism and immunity, differ depending on the types of antibiotics, and the antibiotic-induced gut microbiota alteration has a correlation with host physiology such as host metabolic or immunological status. Thus, the immune and metabolic status of the host should be taken into account when administering antibiotics.
Improved Extraction Efficiency and Antioxidant Activity of Defatted Canola Meal Extract Phenolic Compounds Obtained from Air-Fried Seeds
Authors: Fadairo Olamide S. , Nandasiri Ruchira , Nguyen Thu , Eskin N. A Michael , Aluko Rotimi E. , Scanlon Martin G.
Published: December 2022

This study investigated the efficacy of roasting pre-treatment by air frying to enhance the extraction and recovery of the predominant sinapic acid derivatives (SADs) from roasted canola meal and the antioxidant potential of the methanolic extracts. Canola meal was obtained by air frying canola seed at 160, 170, 180 or 190 °C for 5, 10, 15 or 20 min. Oil was extracted using the Soxhlet method, and the de-oiled meal fraction was air-dried. Phenolic compounds were isolated using ultrasound-assisted extraction with 70% (v/v) methanol and then quantified by high-performance liquid chromatography-diode array detection. The antioxidant potential of the defatted meal methanolic extracts was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and metal ion-chelating activity (MIC) assays. The highest total phenolic content of 3.15 mg gallic acid equivalent/g dry weight was recorded in the defatted meal extract from seeds pre-treated with air frying at 190 °C for 15 min. Sinapine, sinapic acid and an unknown compound at a retention time (RT) of 26.6 min were the major sinapates identified in the defatted meal with the highest concentrations of 7572 ± 479.2 µg/g DW, 727 ± 43.45 µg/g DW and 1763 ± 73.5 µg/g DW, respectively, obtained at 160 °C for 5 min. Canolol (151.35 ± 7.65 µg/g DW) was detected after air frying at a temperature of 170 °C for 20 min. The FRAP and MIC correlated positively (r = 0.85) and generally decreased with increased air frying temperature-time conditions. The highest FRAP and MIC values of 0.53 mM and 80% were obtained at 160 °C for 5 and 20 min, respectively. The outcome of this study will contribute new knowledge that could improve the value addition and by-product utilization of canola seeds.
Doxycycline Attenuated Ethanol-Induced Inflammaging in Endothelial Cells: Implications in Alcohol-Mediated Vascular Diseases
Authors: Li Xuanchen , Khan Dilaware , Rana Majeed , Hänggi Daniel , Muhammad Sajjad
Published: December 2022

Excess alcohol consumption is a potential risk factor for cardiovascular diseases and is linked to accelerated aging. Drug discovery to reduce toxic cellular events of alcohol is required. Here, we investigated the effects of ethanol on human umbilical vein endothelial cells (HUVECs) and explored if doxycycline attenuates ethanol-mediated molecular events in endothelial cells. Initially, a drug screening using a panel of 170 drugs was performed, and doxycycline was selected for further experiments. HUVECs were treated with different concentrations (300 mM and 400 mM) of ethanol with or without doxycycline (10 µg/mL). Telomere length was quantified as telomere to single-copy gene (T/S) ratio. Telomere length and the mRNA expression were quantified by qRT-PCR, and protein level was analyzed by Western blot (WB). Ethanol treatment accelerated cellular aging, and doxycycline treatment recovered telomere length. Pathway analysis showed that doxycycline inhibited mTOR and NFκ-B activation. Doxycycline restored the expression of aging-associated proteins, including lamin b1 and DNA repair proteins KU70 and KU80. Doxycycline reduced senescence and senescence-associated secretory phenotype (SASP) in ethanol-treated HUVECs. In conclusion, we report that ethanol-induced inflammation and aging in HUVECs were ameliorated by doxycycline.