bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations of vulnerability to stressful life events with suicide attempts after active duty among high-risk soldiers: results from the Study to Assess Risk and Resilience in Servicemembers-longitudinal study (STARRS-LS)
Authors: Chu Carol , Stanley Ian H. , Marx Brian P. , King Andrew J. , Vogt Dawne , Gildea Sarah M. , Hwang Irving H. , Sampson Nancy A. , O'Brien Robert , Stein Murray B. , Ursano Robert J. , Kessler Ronald C.
Published: May 2022

Background

The transition from military service to civilian life is a high-risk period for suicide attempts (SAs). Although stressful life events (SLEs) faced by transitioning soldiers are thought to be implicated, systematic prospective evidence is lacking.

Methods

Participants in the Army Study to Assess Risk and Resilience in Servicemembers (STARRS) completed baseline self-report surveys while on active duty in 2011–2014. Two self-report follow-up Longitudinal Surveys (LS1: 2016–2018; LS2: 2018–2019) were subsequently administered to probability subsamples of these baseline respondents. As detailed in a previous report, a SA risk index based on survey, administrative, and geospatial data collected before separation/deactivation identified 15% of the LS respondents who had separated/deactivated as being high-risk for self-reported post-separation/deactivation SAs. The current report presents an investigation of the extent to which self-reported SLEs occurring in the 12 months before each LS survey might have mediated/modified the association between this SA risk index and post-separation/deactivation SAs.

Results

The 15% of respondents identified as high-risk had a significantly elevated prevalence of some post-separation/deactivation SLEs. In addition, the associations of some SLEs with SAs were significantly stronger among predicted high-risk than lower-risk respondents. Demographic rate decomposition showed that 59.5% (s.e. = 10.2) of the overall association between the predicted high-risk index and subsequent SAs was linked to these SLEs.

Conclusions

It might be possible to prevent a substantial proportion of post-separation/deactivation SAs by providing high-risk soldiers with targeted preventive interventions for exposure/vulnerability to commonly occurring SLEs.
Precaution and proportionality in pandemic politics: democracy, state capacity, and COVID-19-related school closures around the world
Authors: Cronert Axel
Published: May 2022

The COVID-19 pandemic triggered a globally spread—but differently timed—implementation of school closures and other disruptive containment measures as governments worldwide intervened to curb transmission of disease. This study argues that the timing of such disruptive interventions reflects how governments balance the principles of precaution and proportionality in their pandemic decision-making. A theory is proposed of how their trade-off is impacted by two interacting institutional factors: electoral democratic institutions, which incentivise political leaders to increasingly favour precaution, and high state administrative capacity, which instead makes a proportional strategy involving later containment measures more administratively and politically feasible. Global patterns consistent with this theory are documented among 170 countries in early 2020, using Cox models of school closures and other non-pharmaceutical interventions. Corroborating the theorised mechanisms, additional results indicate that electoral competition prompts democratic leaders’ faster response, and that this mechanism is weaker where professional state agencies have more influence over policymaking.
DEFINING RURALITY AT LA JOYANCA (PETEN, GUATEMALA): ARCHITECTURE, LAND USE, AND SOCIAL DYNAMICS
Authors: Lemonnier Eva , Arnauld M. Charlotte
Published: May 2022

Considering the Classic Maya lowlands as an intricate landscape of nested settlements with cities, villages, and farmsteads in the middle of agricultural land, the rural/urban conceptual contrast would apparently apply, yet, is still debated. By combining detailed studies of the relationships between populations of both categories, one can better understand what rurality and urbanity meant in ancient Maya societies, and evaluate the dichotomy. Judging by the spatial distribution of architecture and the social dynamics, rural/urban relationships would have reached beyond the scope of agro-economies. Based on the study of La Joyanca (Peten, Guatemala), a medium-sized settlement surrounded by villages and hamlets, this article explores the topic of rurality as contrasted with urbanity through the parameters of potential land use, visible architectural variation, and plausible population mobility. We aim at assessing the relationships between the center and its hinterland as an attempt at furthering the implied concepts.
HEALTH AMONG CLASSIC-PERIOD URBAN AND RURAL MAYA: A REGIONAL PERSPECTIVE
Authors: Tiesler Vera , López Pérez Raúl
Published: May 2022

Health benefits among the members of state-level societies may vary depending on sex, social privilege, and whether the individual resides in an urban or rural setting. Human skeletal remains are prone to express individual life experiences and, ultimately, well-being. This research elaborates on these correlates by contextualizing the physiological stresses among Classic Maya hinterland populations in comparison to their urban peers. Comparisons are made using the frequencies and expression of enamel hypoplasia, caries, porotic hyperostosis, infectious osteomyelitis/subperiosteal reaction and osteoporosis in 842 adult skeletons of both sexes from 63 peripheral and centric, inland, lowland settlements. The results suggest problematic inland weaning diets and higher infectious load among rural populations. While comparisons between urban and rural lifeways show inconsistent load differences, our results indicate repeated distinctions between the sexes. We cautiously interpret this pattern as an indication of a physically demanding regime of rural life compared to a more sedentary routine among urban peers and gendered lifestyles in general. We conclude that apart from these distinctions (and potential sample biases), the health costs versus benefits impacted rural lifestyles in a complex and non-uniform fashion during the first millenniuma.d., rejecting clear-cut hierarchical conceptualizations while inviting more nuanced causal explorations.
RURAL SOCIAL DIFFERENTIATION IN EARLY CLASSIC CHUNHUAYUM, YUCATAN, MEXICO
Authors: Lamb Céline
Published: May 2022

This article examines rural social differentiation in Chunhuayum, Yucatan, a rural village continuously occupied from approximately 800 b.c.–a.d. 1000. Focusing on the late Early Classic (a.d. 400/500–600/630), a time when other settlements of the Uci polity experienced political and population disruptions, I examine how households shaped and expressed local social differentiation, particularly wealth, occupation, and social connectivity. Residential architecture provides the most salient marker of wealth differences at Chunhuayum, while ceramic, shell, and obsidian assemblages indicate that households also varied in terms of their occupations and external social networks. Within this predominantly agrarian village, two households attempted to improve their economic and immaterial well-being through locally innovative strategies—shell crafting and group-oriented ritual orchestration. Such strategies ultimately had different outcomes both for the household and community. These points underscore the heterogeneity of the rural ancient rural Maya, and that social differentiation was actively constructed by rural people rather than a trickling-down of the normative hierarchical social order. Through habituated practice and innovative action, Chunhuayum's Early Classic residents continued participating in external networks while shaping locally meaningful relations of differences.
Turning Science into Fiction? Censoring Population Research in the Soviet Union, 1964–1982
Authors: Lovett Jessica
Published: May 2022

Population statistics reflect a nation's quality of life and accordingly have the potential to be highly politically charged, with implications for a government's legitimacy. In the Brezhnev era Soviet Union, emerging negative trends regarding life expectancy, fertility and mortality had the potential to de-legitimise the Soviet regime just at the moment when population issues were taking the spotlight through the United Nations. For this reason, population statistics were subject to significant censorship. The article examines how this censorship worked for domestic and international audiences. I show the main form of censorship was an editorial review by trusted experts in the Party and argue that the process was defined by uncertainty and negotiation, with personal networks mediating the result. In general, the period was characterised by tension between the need to expand demographic research and leaders' desire to suppress knowledge of unfortunate demographic truths.
The Origins of Euratom's Research on Controlled Thermonuclear Fusion: Cold War Politics and European Integration, 1958–1968
Authors: Curli Barbara
Published: May 2022

The article traces the origins and early developments of European fusion research in the framework of Euratom and of postwar nuclear international institutionalism, and as an episode of both the technoscientific Cold War and the interaction between big science and politics in the history of European integration. Based on original Italian, French and European Union archival sources, the article deals with four main passages of Euratom's fusion history: the Euratom treaty and Euratom's first five-year programme (1958–62); the early attempts to establish a Euratom ‒ CERN Joint Study Group for Fusion Research (1958‒9); the launching of Euratom's first fusion programme; and the contribution of Euratom's ‘fusion association contracts’ with the member states to the creation and training of a European transnational epistemic community of fusion scientists and technocrats. The Merger Treaty of 1965, the ‘crisis’ of Euratom and the prospect of British entry in the Community, as well as the ‘tokamak revolution’ of the late 1960s, would contribute to substantially redefine the European fusion programme.
Effectiveness of a worksite lifestyle intervention to reduce BMI among farmworkers in California: a cluster randomised controlled trial
Authors: Matias Susana L , Riden Heather E , Lee Deandra S , Bang Heejung , Schenker Marc B
Published: May 2022

Objective:

To evaluate the effectiveness of PASOS SALUDABLES, a culturally tailored lifestyle intervention to prevent obesity and diabetes among Latino farmworkers, when implemented at large scale in the worksite.

Design:

This study was a two-arm parallel group, cluster randomised controlled trial, where participants received either a twelve-session lifestyle intervention (intervention) or six-session leadership training (control) at their worksite. The intervention was delivered by Promotoras in Spanish. All sessions were conducted at the worksites (ranches) during meal breaks. Blinded, trained research assistants collected socio-demographic and outcome data (i.e. BMI as primary outcome and waist circumference, glycated Hb (HbA1c), cholesterol and blood pressure as secondary outcomes) at baseline and follow-up assessments (i.e. 3 months, 6 months, 1 year and 1·5 years).

Setting:

Recruitment and intervention delivery occurred at twelve study ranches in Oxnard, California.

Participants:

We enrolled farmworkers hired by a large berry grower company, who were ≥18 years old, spoke Spanish and were free of diabetes at screening.

Results:

A total of 344 workers were enrolled in the intervention and 271 in the control group. The intervention resulted in attenuated increase of BMI over time; however, the difference in trend between groups was not significant (β = −0·01 for slope difference, P = 0·29). No significantly different trend by group was observed in secondary outcomes (P > 0·27).

Conclusions:

The worksite intervention, implemented during meal breaks, did not reduce BMI or other clinical indicators. Nevertheless, this study supports the feasibility of recruiting and engaging the Latino farmworker population in workplace health promotion interventions.
Novellas and Free Voluntary Reading: an overview and some starting points for further research into practice
Authors: Hunt Steven
Published: May 2022

In the US the use of extensive reading in Latin for school students to develop vocabulary growth and attain reading fluency is an emerging classroom practice. To meet the demand for resources, a small but growing number of teachers have started to make available self-published short Latin stories or ‘novellas’, designed to provide students with extensive reading material in Latin at an age-appropriate level for novice and intermediate level students. There are now over 100 such short books, written in Latin, on a variety of topics, suitable for beginner and intermediate students (typically of between one and two years taught Latin). This article contains three areas of interest: the rationale, challenges and successes of writing and using these resources in the school classroom; a snapshot survey of a range of publications, to give readers a ‘flavour’ of the materials; and some further reading.
Younger and Older Adult Reports of Affect in Familiar and Unfamiliar Persons
Authors: Ready Rebecca E. , Santorelli Gennarina D. , Mather Molly A. , Laws Holly
Published: May 2022

Abstract. Little is known about how people perceive the affective well-being of older and younger adults. In four studies, we compare other- and self-reported trait affect from participants who varied in adult age and familiarity. Our prediction that older adults would be rated by other persons as having poorer well-being than their self-report was not supported. Rather, we found that younger persons were thought by familiar older adults to have better well-being than the young persons reported about themselves; this was not the case for younger familiar raters. Unfamiliar younger adults were rated by younger and older participants as having greater arousal affect than unfamiliar older adults. Misperceptions of well-being may interfere with cross-generation communication and social interactions.
Predictive Skill of Teleconnection Patterns in Twentieth Century Seasonal Hindcasts and Their Relationship to Extreme Winter Temperatures in Europe
Authors: Schuhen Nina , Schaller Nathalie , Bloomfield Hannah C. , Brayshaw David J. , Lledó Llorenç , Cionni Irene , Sillmann Jana
Published: May 2022

European winter weather is dominated by several low‐frequency teleconnection patterns, the main ones being the North Atlantic Oscillation, East Atlantic, East Atlantic/Western Russia, and Scandinavian patterns. We analyze the century‐long ERA‐20C reanalysis and ASF‐20C seasonal hindcast data sets and find that these patterns are subject to decadal variability and fluctuations in predictive skill. Using indices for determining periods of extreme cold or warm temperatures, we establish that the teleconnection patterns are, for some regions, significantly correlated or anti‐correlated to cold or heat waves. The seasonal hindcasts are however only partly able to capture these relationships. There do not seem to be significant changes to the observed links between large‐scale circulation patterns and extreme temperatures between periods of higher and lower predictive skill.
The Influence of Dust‐Smoke Mixtures on Boundary Layer Processes and Nocturnal Warming in the Sahel
Authors: Phillips Christopher , Nair Udaysankar , Christopher Sundar
Published: May 2022

Using a single column model with ground‐based, aircraft, and satellite data sets we assess the combined role of smoke and dust aerosols, land degradation/aridization (LDA), and their impact on the planetary boundary layer (PBL) in influencing near‐surface air temperature over the Sahel. Our study is unique because it assesses the combined role of smoke and dust aerosols on PBL evolution and near‐surface air temperatures during both day and nighttime. More importantly, using a theoretical framework, we provide a careful explanation of the geophysical processes responsible for the changes in PBL and near‐surface air temperature. Our results indicate that during northern hemisphere winter months, dust, and smoke over Sahel radiatively combine to impact the PBL. We show that aerosol mixtures dominated by dust modify PBL height in a manner that minimizes/maximizes surface layer cooling/warming at times when daytime maximum/nocturnal minimum temperatures occur. Furthermore, we find that increasing smoke contribution to total column aerosol optical extinction counteracts nighttime warming through daytime cooling. When smoke constitutes half or more of to the total column aerosol optical extinction, the ratio of longwave to shortwave radiative forcing is less than 10%, and nighttime cooling ensues. Minimum temperature is most sensitive to changes in mid‐visible aerosol optical depth (AOD) values <1 and doubling of dust AOD within this range during the 1950–1980 Sahelian LDA event is estimated to have a nocturnal warming potential of 0.6°C.
The Role of Clouds in Shaping Tropical Pacific Response Pattern to Extratropical Thermal Forcing
Authors: Hsiao Wei‐Ting , Hwang Yen‐Ting , Chen Yong‐Jhih , Kang Sarah M.
Published: May 2022

Extratropical influences on tropical sea surface temperature (SST) have implications for decadal predictability. We implement a cloud‐locking technique to highlight the critical role of clouds in shaping the tropical SST response to extratropical thermal forcing. With heating imposed over either the extratropical Northern Atlantic or Pacific, Hadley Cells respond similarly that the trades strengthen south of the rainband. The wind‐evaporation‐SST (WES) feedback leads to cooling over the southern subtropics, which is enhanced in the southeastern Pacific due to the positive feedback between SST and stratiform clouds. Cloud‐locking experiments show that zonal contrasts in SST and cloud feedbacks in the Pacific enhance the zonal surface winds, leading to increased evaporation and strengthens zonal SST difference. We propose that the meridional and zonal SST gradients are tightly linked via WES effects and the cloud‐radiative‐SST feedbacks, which are largely determined by the climatological rainband position and the spatial distribution of cloud properties.
Sensitive Dependence of Global Climate to Continental Geometry
Authors: Baum Mark , Fu Minmin , Bourguet Stephen
Published: May 2022

Over its multibillion‐year history, the Earth has experienced a wide range of climates. The long‐term climate is controlled by the atmospheric carbon dioxide concentration, which is regulated by marine sequestration through chemical weathering. This chemical weathering sink is strongly linked to the distribution and composition of the continents. However, the effect of continental distribution has never been studied within a general framework. Here we show that the global weathering rate is sensitive to the size and shape of the continents, but is not well explained by the amount of land in the tropics. We construct synthetic continental configurations and use an ensemble of global climate model simulations to isolate the expected effect of continental arrangement on weathering and carbon burial. Runoff patterns are complex, sensitive to detailed features of continental geometry, and poorly predicted by continental latitude. These results help explain the long‐term variability and irregularity of Earth's climate.
Intense Equatorial Electrojet and Counter Electrojet Caused by the 15 January 2022 Tonga Volcanic Eruption: Space‐ and Ground‐Based Observations
Authors: Le Guan , Liu Guiping , Yizengaw Endawoke , Englert Christoph R.
Published: May 2022

We present space‐ and ground‐based multi‐instrument observations demonstrating the impact of the 2022 Tonga volcanic eruption on dayside equatorial electrodynamics. A strong counter electrojet (CEJ) was observed by Swarm and ground‐based magnetometers on 15 January after the Tonga eruption and during the recovery phase of a moderate geomagnetic storm. Swarm also observed an enhanced equatorial electrojet (EEJ) preceding the CEJ in the previous orbit. The observed EEJ and CEJ exhibited complex spatiotemporal variations. We combine them with the Ionospheric Connection Explorer neutral wind measurements to disentangle the potential mechanisms. Our analysis indicates that the geomagnetic storm had minimal impact; instead, a large‐scale atmospheric disturbance propagating eastward from the Tonga eruption site was the most likely driver for the observed intensification and directional reversal of the equatorial electrojet. The CEJ was associated with strong eastward zonal winds in the E‐region ionosphere, as a direct response to the lower atmosphere forcing.
The role of autophagy in cadmium-induced acute toxicity in glomerular mesangial cells and tracking polyubiquitination of cytoplasmic p53 as a biomarker
Authors: Jung Ki-Tae , Oh Seon-Hee
Published: May 2022

Cadmium (Cd) is a highly toxic environmental pollutant that can severely damage the kidneys. Here, we show that Cd-induced apoptosis is promoted by the cytoplasmic polyubiquitination of p53 (polyUb-p53), which is regulated by the polyubiquitination of SQSTM1/p62 (polyUb-p62) and autophagy in mouse kidney mesangial cells (MES13E cells). p53 was detected in monomeric and different high-molecular-weight (HMW) forms after Cd exposure. Monomeric p53 levels decreased in a concentration- and time-dependent manner. HMW-p53 transiently accumulated in the cytoplasm independent of proteasome inhibition. The expression patterns of p53 were similar to those of p62 upon Cd exposure, and the interactions between polyUb-p53 and polyUb-p62 were observed using immunoprecipitation. P62 knockdown reduced polyUb-p53 and upregulated nuclear monomeric p53, whereas p53 knockdown reduced polyUb-p62. Autophagy inhibition induced by ATG5 knockdown reduced Cd-induced polyUb-p62 and polyUb-p53 but upregulated the levels of nuclear p53. Pharmacological inhibition of autophagy by bafilomycin A1 increased polyUb-p62 and polyUb-p53 in the cytoplasm, indicating that p53 protein levels and subcellular localization were regulated by polyUb-p62 and autophagy. Immunoprecipitation and immunofluorescence revealed an interaction between p53 and LC3B, indicating that p53 was taken up by autophagosomes. Cd-resistant RMES13E cells and kidney tissues from mice continuously injected with Cd had reduced polyUb-p53, polyUb-p62, and autophagy levels. Similar results were observed in renal cell carcinoma cell lines. These results indicate that cytoplasmic polyUb-p53 is a potential biomarker for Cd-induced acute toxicity in mesangial cells. In addition, upregulation of nuclear p53 may protect cells against Cd cytotoxicity, but abnormal p53 accumulation may contribute to tumor development.
Bringing chiral functionality to in vivo applications of nanomaterials
Authors: Mukhina Maria
Published: May 2022

Chirality is a universal property of an endless number of objects in the universe. Nanotechnology is rapidly expanding to find ways to introduce chirality to artificial nanostructures. In a recent publication in Light: Science and Applications, Das et al. have successfully used capping with chiral ligand molecules to obtain chiral carbon dots. The authors provide a theoretical model to describe the origin of chirality in carbon dots as arising due to exciton coupling in a pair of chiral chromophores. Due to non-toxic chemical composition and sizes as small as 2–5 nm, the chiral carbon dots have the potential to outperform other chiral nanostructures in numerous biomedical applications. However, similarly to chiral drugs, their chiral toxicity must be well understood before the carbon dots are brought to living systems.
Metasurface-enabled on-chip multiplexed diffractive neural networks in the visible
Authors: Luo Xuhao , Hu Yueqiang , Ou Xiangnian , Li Xin , Lai Jiajie , Liu Na , Cheng Xinbin , Pan Anlian , Duan Huigao
Published: May 2022

Replacing electrons with photons is a compelling route toward high-speed, massively parallel, and low-power artificial intelligence computing. Recently, diffractive networks composed of phase surfaces were trained to perform machine learning tasks through linear optical transformations. However, the existing architectures often comprise bulky components and, most critically, they cannot mimic the human brain for multitasking. Here, we demonstrate a multi-skilled diffractive neural network based on a metasurface device, which can perform on-chip multi-channel sensing and multitasking in the visible. The polarization multiplexing scheme of the subwavelength nanostructures is applied to construct a multi-channel classifier framework for simultaneous recognition of digital and fashionable items. The areal density of the artificial neurons can reach up to 6.25 × 106 mm−2 multiplied by the number of channels. The metasurface is integrated with the mature complementary metal-oxide semiconductor imaging sensor, providing a chip-scale architecture to process information directly at physical layers for energy-efficient and ultra-fast image processing in machine vision, autonomous driving, and precision medicine.
An investigation into the reliability of a mobile app designed to assess orthodontic treatment need and severity
Authors: Nandra Sukbir , Crawford Nicola , Burford Daniel , Pandis Nikolaos , Cobourne Martyn T. , Seehra Jadbinder
Published: May 2022

Aim To investigate reliability of the Easy IOTN app between clinicians with different levels of experience in determining Index of Orthodontic Treatment Need (IOTN) Dental Health Component (DHC) and Aesthetic Component (AC) scores from study models. The accuracy of each clinician in discriminating treatment need using the app against the 'gold standard' conventional assessment at the threshold of treatment acceptance criteria was also explored.

Materials and methods In total, 150 sets of pre-treatment study models were assessed by six clinicians using the app on two separate occasions (T1 and T2). A single IOTN-calibrated clinician also scored the models using the conventional technique. Clinician scores for both intra- and inter-rater reliability were assessed using Cohen's Kappa. The performance of each clinician in discriminating treatment need using the app against the conventional assessment method at the threshold of treatment acceptance criteria was also assessed using the area under the curve-receiver operating characteristic.

Results The intra-rater agreement for the clinician undertaking the conventional assessment of the models was 1.0. Intra-rater agreement scores for clinicians using the Easy IOTN app ranged between 0.37-0.87 (DHC) and 0.22-0.44 (AC). Inter-rater agreement scores at T2 were 0.59 (DHC) and 0.23 (AC). Based on the IOTN DHC, all clinicians displayed an excellent level of accuracy in determining malocclusions qualifying for treatment (range 81.7-90.0%). Based on the IOTN AC, all clinicians showed an acceptable level of accuracy in determining malocclusions qualifying for treatment (range 71.9-79.2%).

Conclusions The Easy IOTN app was shown to have moderate inter-rater reliability. Variation in the intra-rater reliability was evident between clinicians of different grades/level of experience. Importantly, the diagnostic accuracy of the app to discriminate between malocclusions that qualify for NHS treatment was rated as excellent (IOTN DHC) and acceptable (IOTN AC) and independent of clinician grade or level of experience.
Prosocial correlates of transformative experiences at secular multi-day mass gatherings
Authors: Yudkin Daniel A. , Prosser Annayah M. B. , Heller S. Megan , McRae Kateri , Chakroff Aleksandr , Crockett M. J.
Published: May 2022

Humans have long sought experiences that transcend or change their sense of self. By weakening boundaries between the self and others, such transformative experiences may lead to enduring changes in moral orientation. Here we investigated the psychological nature and prosocial correlates of transformative experiences by studying participants before (n = 600), during (n = 1217), 0–4 weeks after (n = 1866), and 6 months after (n = 710) they attended a variety of secular, multi-day mass gatherings in the US and UK. Observations at 6 field studies and 22 online followup studies spanning 5 years showed that self-reported transformative experiences at mass gatherings were common, increased over time, and were characterized by feelings of universal connectedness and new perceptions of others. Participants’ circle of moral regard expanded with every passing day onsite—an effect partially mediated by transformative experience and feelings of universal connectedness. Generosity was remarkably high across sites but did not change over time. Immediately and 6 months following event attendance, self-reported transformative experience persisted and predicted both generosity (directly) and moral expansion (indirectly). These findings highlight the prosocial qualities of transformative experiences at secular mass gatherings and suggest such experiences may be associated with lasting changes in moral orientation.
Dynamic hetero-metallic bondings visualized by sequential atom imaging
Authors: Inazu Minori , Akada Yuji , Imaoka Takane , Hayashi Yoko , Takashima Chinami , Nakai Hiromi , Yamamoto Kimihisa
Published: May 2022

Traditionally, chemistry has been developed to obtain thermodynamically stable and isolable compounds such as molecules and solids by chemical reactions. However, recent developments in computational chemistry have placed increased importance on studying the dynamic assembly and disassembly of atoms and molecules formed in situ. This study directly visualizes the formation and dissociation dynamics of labile dimers and trimers at atomic resolution with elemental identification. The video recordings of many homo- and hetero-metallic dimers are carried out by combining scanning transmission electron microscopy (STEM) with elemental identification based on the Z-contrast principle. Even short-lived molecules with low probability of existence such as AuAg, AgCu, and AuAgCu are directly visualized as a result of identifying moving atoms at low electron doses.
Fluent molecular mixing of Tau isoforms in Alzheimer’s disease neurofibrillary tangles
Authors: Dregni Aurelio J. , Duan Pu , Xu Hong , Changolkar Lakshmi , El Mammeri Nadia , Lee Virginia M.-Y. , Hong Mei
Published: May 2022

Alzheimer’s disease (AD) is defined by intracellular neurofibrillary tangles formed by the microtubule-associated protein tau and extracellular plaques formed by the β-amyloid peptide. AD tau tangles contain a mixture of tau isoforms with either four (4R) or three (3R) microtubule-binding repeats. Here we use solid-state NMR to determine how 4R and 3R tau isoforms mix at the molecular level in AD tau aggregates. By seeding differentially isotopically labeled 4R and 3R tau monomers with AD brain-derived tau, we measured intermolecular contacts of the two isoforms. The NMR data indicate that 4R and 3R tau are well mixed in the AD-tau seeded fibrils, with a 60:40 incorporation ratio of 4R to 3R tau and a small homotypic preference. The AD-tau templated 4R tau, 3R tau, and mixed 4R and 3R tau fibrils exhibit no structural differences in the rigid β-sheet core or the mobile domains. Therefore, 4R and 3R tau are fluently recruited into the pathological fold of AD tau aggregates, which may explain the predominance of AD among neurodegenerative disorders.
NLRP3 licenses NLRP11 for inflammasome activation in human macrophages
Authors: Gangopadhyay Anu , Devi Savita , Tenguria Shivendra , Carriere Jessica , Nguyen Huyen , Jäger Elisabeth , Khatri Hemisha , Chu Lan H. , Ratsimandresy Rojo A. , Dorfleutner Andrea , Stehlik Christian
Published: May 2022

Intracellular sensing of stress and danger signals initiates inflammatory innate immune responses by triggering inflammasome assembly, caspase-1 activation and pyroptotic cell death as well as the release of interleukin 1β (IL-1β), IL-18 and danger signals. NLRP3 broadly senses infectious patterns and sterile danger signals, resulting in the tightly coordinated and regulated assembly of the NLRP3 inflammasome, but the precise mechanisms are incompletely understood. Here, we identified NLRP11 as an essential component of the NLRP3 inflammasome in human macrophages. NLRP11 interacted with NLRP3 and ASC, and deletion of NLRP11 specifically prevented NLRP3 inflammasome activation by preventing inflammasome assembly, NLRP3 and ASC polymerization, caspase-1 activation, pyroptosis and cytokine release but did not affect other inflammasomes. Restored expression of NLRP11, but not NLRP11 lacking the PYRIN domain (PYD), restored inflammasome activation. NLRP11 was also necessary for inflammasome responses driven by NLRP3 mutations that cause cryopyrin-associated periodic syndrome (CAPS). Because NLRP11 is not expressed in mice, our observations emphasize the specific complexity of inflammasome regulation in humans.
Proteomic and transcriptomic profiles of human urothelial cancer cells with histone deacetylase 5 overexpression
Authors: Jaguva Vasudevan Ananda Ayyappan , Hoffmann Michèle J. , Poschmann Gereon , Petzsch Patrick , Wiek Constanze , Stühler Kai , Köhrer Karl , Schulz Wolfgang A. , Niegisch Günter
Published: May 2022

Urothelial carcinoma (UC) of the urinary bladder is a prevalent cancer worldwide. Because histone deacetylases (HDACs) are important factors in cancer, targeting these epigenetic regulators is considered an attractive strategy to develop novel anticancer drugs. Whereas HDAC1 and HDAC2 promote UC, HDAC5 is often downregulated and only weakly expressed in UC cell lines, suggesting a tumor-suppressive function. We studied the effect of stable lentiviral-mediated HDAC5 overexpression in four UC cell lines with different phenotypes (RT112, VM-Cub-1, SW1710, and UM-UC-3, each with vector controls). In particular, comprehensive proteomics and RNA-seq transcriptomics analyses were performed on the four cell line pairs, which are described here. For comparison, the immortalized benign urothelial cell line HBLAK was included. These datasets will be a useful resource for researchers studying UC, and especially the influence of HDAC5 on epithelial-mesenchymal transition (EMT). Moreover, these data will inform studies on HDAC5 as a less studied member of the HDAC family in other cell types and diseases, especially fibrosis.
Perceptions and behavior of clinical researchers and research support staff regarding data FAIRification
Authors: Kersloot Martijn G. , Abu-Hanna Ameen , Cornet Ronald , Arts Derk L.
Published: May 2022

The FAIR Data Principles are being rapidly adopted by many research institutes and funders worldwide. This study aimed to assess the awareness and attitudes of clinical researchers and research support staff regarding data FAIRification. A questionnaire was distributed to researchers and support staff in six Dutch University Medical Centers and Electronic Data Capture platform users. 164 researchers and 21 support staff members completed the questionnaire. 62.8% of the researchers and 81.0% of the support staff are currently undertaking at least some effort to achieve any aspect of FAIR, 11.0% and 23.8%, respectively, address all aspects. Only 46.6% of the researchers add metadata to their datasets, 39.7% add metadata to data elements, and 35.9% deposit their data in a repository. 94.7% of the researchers are aware of the usefulness of their data being FAIR for others and 89.3% are, given the right resources and support, willing to FAIRify their data. Institutions and funders should, therefore, develop FAIRification training and tools and should (financially) support researchers and staff throughout the process.
Multi sequence average templates for aging and neurodegenerative disease populations
Authors: Dadar Mahsa , Camicioli Richard , Duchesne Simon
Published: May 2022

Magnetic resonance image (MRI) processing pipelines use average templates to enable standardization of individual MRIs in a common space. MNI-ICBM152 is currently used as the standard template by most MRI processing tools. However, MNI-ICBM152 represents an average of 152 healthy young adult brains and is vastly different from brains of patients with neurodegenerative diseases. In those populations, extensive atrophy might cause inevitable registration errors when using an average template of young healthy individuals for standardization. Disease-specific templates that represent the anatomical characteristics of the populations can reduce such errors and improve downstream driven estimates. We present multi-sequence average templates for Alzheimer’s Dementia (AD), Fronto-temporal Dementia (FTD), Lewy Body Dementia (LBD), Mild Cognitive Impairment (MCI), cognitively intact and impaired Parkinson’s Disease patients (PD-CIE and PD-CI, respectively), individuals with Subjective Cognitive Impairment (SCI), AD with vascular contribution (V-AD), Vascular Mild Cognitive Impairment (V-MCI), Cognitively Intact Elderly (CIE) individuals, and a human phantom. We also provide separate templates for males and females to allow better representation of the diseases in each sex group.
Spatial and temporal data to study residential heat decarbonisation pathways in England and Wales
Authors: Canet Alexandre , Qadrdan Meysam , Jenkins Nick , Wu Jianzhong
Published: May 2022

The decarbonisation of residential heating is crucial if the net-zero target in the United Kingdom is going to be achieved. This paper describes methods to produce data to quantify the impacts of residential heat decarbonisation on the energy supply infrastructure across England and Wales. For the year 2018, annual heat demand for a range of dwellings was estimated for almost 35,000 local areas (known as Lower Layer Super Output Areas: LSOAs). Energy savings through implementing the potential energy efficiency measures and the indicative costs of the energy efficiency measures were quantified. Profiles were synthesised for heat production and energy demand of selected heating technologies using average daily temperature and data from trial projects. These profiles were created to study the impacts of different types of heating technology in each LSOA under user-defined heat decarbonisation pathways. Data describing the dwelling stock, heating technologies, annual heat demand for each LSOA, indicative costs of energy efficiency improvements for each local authority and the profiles for each technology were created.
Multi-cultural cities reduce disadvantages in recognizing naturalistic images of other-race faces: evidence from a novel face learning task
Authors: Zhou Xiaomei , Mondloch Catherine J. , Chien Sarina Hui-Lin , Moulson Margaret C.
Published: May 2022

People often find it more difficult to recognize other- than own-race faces. This other-race effect is robust across numerous ethnic groups. Yet, it remains unclear how this effect changes in people who live in a multiracial environment, and in immigrants whose lifetime perceptual experience changes over time. In the present study, we developed a novel face recognition test that approximates face recognition in the real world. We tested five groups of White and East Asian adults (n = 120) living in racially homogeneous versus heterogeneous cities and East Asians who immigrated to a multiracial city between infancy and adulthood. Multiracial cities reduce the other-race effect. The magnitude of the other-race effect changes as a function of experience, mirroring the racial diversity in perceivers’ living environment. Our study highlights the challenge of forming reliable face representations across naturalistic facial variability and suggests a facilitative role of multiracial environments in eliminating the other-race effect.
The erythrocyte membrane properties of beta thalassaemia heterozygotes and their consequences for Plasmodium falciparum invasion
Authors: Introini Viola , Marin-Menendez Alejandro , Nettesheim Guilherme , Lin Yen-Chun , Kariuki Silvia N. , Smith Adrian L. , Jean Letitia , Brewin John N. , Rees David C. , Cicuta Pietro , Rayner Julian C. , Penman Bridget S.
Published: May 2022

Malaria parasites such as Plasmodium falciparum have exerted formidable selective pressures on the human genome. Of the human genetic variants associated with malaria protection, beta thalassaemia (a haemoglobinopathy) was the earliest to be associated with malaria prevalence. However, the malaria protective properties of beta thalassaemic erythrocytes remain unclear. Here we studied the mechanics and surface protein expression of beta thalassaemia heterozygous erythrocytes, measured their susceptibility to P. falciparum invasion, and calculated the energy required for merozoites to invade them. We found invasion-relevant differences in beta thalassaemic cells versus matched controls, specifically: elevated membrane tension, reduced bending modulus, and higher levels of expression of the major invasion receptor basigin. However, these differences acted in opposition to each other with respect to their likely impact on invasion, and overall we did not observe beta thalassaemic cells to have lower P. falciparum invasion efficiency for any of the strains tested.
Children with autism spectrum disorder show atypical electroencephalographic response to processing contextual incongruencies
Authors: Márquez-García Amparo V. , Vakorin Vasily A. , Kozhemiako Nataliia , Magnuson Justine R. , Iarocci Grace , Ribary Urs , Moreno Sylvain , Doesburg Sam M.
Published: May 2022

Children with autism spectrum disorder (ASD) experience difficulties with social communication, making it challenging to interpret contextual information that aids in accurately interpreting language. To investigate how the brain processes the contextual information and how this is different in ASD, we compared event-related potentials (ERPs) in response to processing visual and auditory congruent and incongruent information. Two groups of children participated in the study: 37 typically developing children and 15 children with ASD (age range = 6 to 12). We applied a language task involving auditory sentences describing congruent or incongruent images. We investigated two ERP components associated with language processing: the N400 and P600. Our results showed how children with ASD present significant differences in their neural responses in comparison with the TD group, even when their reaction times and correct trials are not significantly different from the TD group.
Massive-mode polarization entangled biphoton frequency comb
Authors: Yamazaki Tomohiro , Ikuta Rikizo , Kobayashi Toshiki , Miki Shigehito , China Fumihiro , Terai Hirotaka , Imoto Nobuyuki , Yamamoto Takashi
Published: May 2022

A frequency-multiplexed entangled photon pair and a high-dimensional hyperentangled photon pair are useful to realize a high-capacity quantum communication. A biphoton frequency comb (BFC) with entanglement can be used to prepare both states. We demonstrate polarization entangled BFCs with over 1400 frequency modes, which is approximately two orders of magnitude larger than those of earlier entangled BFCs, by placing a singly resonant periodically poled LiNbO3 waveguide resonator within a Sagnac loop. The BFCs are demonstrated by measuring the joint spectral intensity, cross-correlation, and autocorrelation. Moreover, the polarization entanglement at representative groups of frequency modes is verified by quantum state tomography, where each fidelity is over 0.7. The efficient generation of a massive-mode entangled BFC is expected to accelerate the increase of capacity in quantum communication.
Differences in composition of interdigital skin microbiota predict sheep and feet that develop footrot
Authors: Clifton Rachel , Monaghan Emma M. , Green Martin J. , Purdy Kevin J. , Green Laura E.
Published: May 2022

Footrot has a major impact on health and productivity of sheep worldwide. The current paradigm for footrot pathogenesis is that physical damage to the interdigital skin (IDS) facilitates invasion of the essential pathogen Dichelobacter nodosus. The composition of the IDS microbiota is different in healthy and diseased feet, so an alternative hypothesis is that changes in the IDS microbiota facilitate footrot. We investigated the composition and diversity of the IDS microbiota of ten sheep, five that did develop footrot and five that did not (healthy) at weekly intervals for 20 weeks. The IDS microbiota was less diverse on sheep 2 + weeks before they developed footrot than on healthy sheep. This change could be explained by only seven of > 2000 bacterial taxa detected. The incubation period of footrot is 8–10 days, and there was a further reduction in microbial diversity on feet that developed footrot in that incubation period. We conclude that there are two stages of dysbiosis in footrot: the first predisposes sheep to footrot and the second occurs in feet during the incubation of footrot. These findings represent a step change in our understanding of the role of the IDS microbiota in footrot pathogenesis.
Effects of humic acid on Pb2+ adsorption onto polystyrene microplastics from spectroscopic analysis and site energy distribution analysis
Authors: Lu Xiaotian , Zeng Feng , Wei Shuyin , Gao Rui , Abdurahman Abliz , Wang Hao , Liang Weiqian
Published: May 2022

Microplastics (MPs), act as vectors of heavy metal pollutants in the environment, is of practical significance to study the adsorption process and mechanism on heavy metals. In this study, polystyrene microplastics (PSMPs) were used as model MPs to study the adsorption of Pb2+ on PSMPs and the effects of humic acid (HA) on the adsorption process. The results showed that HA promoted the adsorption of Pb2+ on PSMPs, and the higher the concentration of HA, the greater the adsorption of Pb2+. With the increase of pH value and decrease of ionic strength, the adsorption capacity of PSMPs for Pb2+ increased. The scanning electron microscope equipped with the energy dispersive spectroscope (SEM–EDS), fourier transform-infrared spectra (FT-IR) and X-ray photoelectron spectroscopy (XPS) analysis showed that Pb2+ could be adsorbed directly onto PSMPs and also indirectly by HA. The higher KSV values in the PSMPs-HA-Pb2+ system than PSMPs-HA system by fluorescence analysis of HA suggested that HA acted as a bridging role in the adsorption of Pb2+ on PSMPs. The site energy distribution analysis further revealed that HA increased the average site energy μ(E*) and its standard deviation σe* of PSMPs by introducing more adsorption sites, thus enhanced the adsorption affinity of PSMPs. This study provided more thoughts and insights into the adsorption behavior and mechanism of MPs for Pb2+ in aquatic environments.
A cross-sectional survey on attitudes of men who have sex with men towards anal self-examination for detection of anal syphilis
Authors: Aung Ei T. , Fairley Christopher K. , Ong Jason J. , Phillips Tiffany R. , Chen Marcus Y. , Tran Julien , Maddaford Kate , Rodriguez Elena R. , Chow Eric P. F.
Published: May 2022

Studies suggest men who have sex with men (MSM) practising receptive anal sex are more likely to present with secondary syphilis, implying primary anorectal lesions are likely to be missed. If men could detect anorectal lesions in the primary stage by regular anal self-examination (ASE), transmission could be reduced by early diagnosis and treatment. We aimed to explore the attitudes of MSM on performing ASE to detect primary anorectal syphilis. An online anonymous cross-sectional survey among MSM over 18 years of age living in Australia, was conducted between July and November 2020 and recruitment was from a sexual health clinic and social media. A total of 568 MSM completed the survey (median age: 34 [IQR 27–45]): 32% (183) had previously performed ASE. Among 66% (374) who had never performed ASE, 68% (250) would consider performing ASE in the future with a preferred median frequency of 2 times per 4 weeks (IQR 1–4), whilst men who were already performing ASE were performing it at median 1 per 4 weeks (IQR 0.2–3). Almost two-thirds of MSM who had never performed ASE were willing to adopt ASE practice in the future. Studies are required to determine the effectiveness of ASE for syphilis detection.