bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengembangan Sistem Online Pendaftaran Santri Baru untuk Penguatan Manajemen Informasi Pondok Pesantren Islamiyah Syafi’iyah Situbondo
Authors: Fajri Fathorazi Nur , Shudiq Wali Ja’far , Nasyihin Nurul Hidayattun
Published: January 2021

New Santri Admission (Penerimaan Santri Baru) is an administrative process for the selection of new student candidates. The PSB process is always carried out manually at each school institution, especially the Islamic Boarding Schools, which will cause various difficulties from several related parties. Therefore the New Santri Admission System was created online or the term can be called the online PSB. . An idea emerged to create a new student admission information system based on the SQL database. With this system, pesantren or administrators can manage the implementation of student registration for web technology using the PHP programming language and new languages. such as rapid registration and announcements.
A World on His Shoulders: Nat Cohen, Anglo-EMI and the British Film Industry
Authors: Mayne Laura
Published: January 2021

Despite being one of the most significant players in the British film industry of the 1960s and 1970s, Nat Cohen remains a curiously neglected figure in histories of that era. At Anglo-Amalgamated he oversaw a varied slate of productions, from B-movies and cheap programmers to box-office successes like Ken Loach’s Poor Cow. He greenlit some of the greatest commercial hits of the 1960, including New Wave dramas ( Billy Liar, A Kind of Loving), pop musicals ( Catch Us If You Can) and horror films now widely considered to be classics of British cinema ( Peeping Tom). After Anglo-Amalgamated was acquired as part of EMI’s takeover of the Associated British Pictures Corporation (ABPC), Cohen headed Anglo-EMI, where his business acumen and shrewd commercial instincts led to him being dubbed ‘King Cohen’ by the press and widely recognised as one of the most powerful men in the British film industry. Drawing on recent scholarly work on the role of the producer, this article will explore links between Anglo-ABPC and EMI through the lens of Cohen’s career and distinctive ‘movie mogul’ persona.
EMI and the ‘Pre-heritage’ Period Film
Authors: Monk Claire
Published: January 2021

First coined in the UK in the early 1990s as a new label for an ostensibly new, post-1979 kind and cycle of period cinema, the ‘heritage film’ is now firmly established as a widely used term and category in academic film studies. Although the heritage film’s defining features, ideological character and ontological coherence would remain debated, its status as a ‘new’ category hinges, self-evidently, on the presumption that the films of post-1979 culturally English heritage cinema marked a new departure and were clearly distinct from their pre-Thatcher-era precursors. Yet, paradoxically, the British period/costume films of the preceding decade, the 1970s, have attracted almost no scholarly attention, and none which connects them with the post-1979 British heritage film, nor the 1980s cultural and industry conditions said to have fostered these productions with those of the 1970s. This article pursues these questions through the prism of Britain’s largest film production and distribution entity throughout 1970–86, EMI, and EMI’s place as a significant and sustained, but little-acknowledged, force in British period film production throughout that time. In so doing, the article establishes the case for studying ‘pre-heritage’ period cinema. EMI’s period film output included early proto-heritage films but also ventured notably wider. This field of production is examined within the broader terrain of 1970s British and American period cinema and within wider 1970s UK cinema box-office patterns and cultural trends, attending to commercial logics as well as to genre and the films' positioning in relation to the later heritage film debates.
Association Between Obstructive Sleep Apnea, Its Treatment, and Alzheimer's Disease: Systematic Mini-Review
Authors: Kuo Chih-Yun , Hsiao Hung-Ta , Lo Ing-Hsien , Nikolai Tomas
Published: January 2021

Obstructive sleep apnea (OSA) and Alzheimer's disease (AD) are common in the elderly population. Obstructive sleep apnea that may cause significant changes in the cerebrospinal fluid β-amyloid and T-tau and/or P-tau protein levels is often identified as a risk factor for development of AD. Although the underlying mechanisms of AD are still not fully understood, a hypothesis associating OSA with AD has been already proposed. In this systematic mini-review, we first discuss the recent findings supporting the association of OSA with an increased risk of AD and then provide evidence suggesting the positive effect of OSA treatment on a reduced risk of AD.
Stroke Admissions, Stroke Severity, and Treatment Rates in Urban and Rural Areas During the COVID-19 Pandemic
Authors: Erdur Hebun , Siegerink Bob , Leithner Christoph , Franke Christiana , Lorenz-Meyer Irina , Theen Sarah , Angermaier Anselm , Kinze Stephan , Weber Joachim E. , Rohmann Jessica L. , Scheitz Jan F. , Nolte Christian H. , Endres Matthias , Audebert Heinrich J.
Published: January 2021

Background: Many regions worldwide reported a decline of stroke admissions during the early phase of the coronavirus disease 2019 (COVID-19) pandemic. It remains unclear whether urban and rural regions experienced similar declines and whether deviations from historical admission numbers were more pronounced among specific age, stroke severity or treatment groups.

Methods: We used registry datasets from (a) nine acute stroke hospitals in Berlin, and (b) nine hospitals from a rural TeleNeurology network in Northeastern Germany for primary analysis of 3-week-rolling average of stroke/TIA admissions before and during the COVID-19 pandemic. We compared course of stroke admission numbers with regional cumulative severe acute respiratory syndrome coronavirus 2 (Sars-CoV-2) infections. In secondary analyses, we used emergency department logs of the Berlin Charité University hospital to investigate changes in age, stroke severity, and thrombolysis/thrombectomy frequencies during the early regional Sars-CoV-2 spread (March and April 2020) and compared them with preceding years.

Results: Compared to past years, stroke admissions decreased by 20% in urban and 20-25% in rural hospitals. Deviations from historical averages were observable starting in early March and peaked when numbers of regional Sars-CoV-2 infections were still low. At the same time, average admission stroke severity and proportions of moderate/severe strokes (NIHSS >5) were 20 and 20–40% higher, respectively. There were no relevant deviations observed in proportions of younger patients (<65 years), proportions of patients with thrombolysis, or number of thrombectomy procedures. Stroke admissions at Charité subsequently rebounded and reached near-normal levels after 4 weeks when the number of new Sars-CoV-2 infections started to decrease.

Conclusions: During the early pandemic, deviations of stroke-related admissions from historical averages were observed in both urban and rural regions of Northeastern Germany and appear to have been mainly driven by avoidance of admissions of mildly affected stroke patients.
Exploring Movement Impairments in Patients With Parkinson's Disease Using the Microsoft Kinect Sensor: A Feasibility Study
Authors: Rudå Ditte , Einarsson Gudmundur , Andersen Anne Sofie Schott , Matthiassen Jannik Boll , Correll Christoph U. , Winge Kristian , Clemmensen Line K. H. , Paulsen Rasmus R. , Pagsberg Anne Katrine , Fink-Jensen Anders
Published: January 2021

Background:Current assessments of motor symptoms in Parkinson's disease are often limited to clinical rating scales.

Objectives:To develop a computer application using the Microsoft Kinect sensor to assess performance-related bradykinesia.

Methods:The developed application (Motorgame) was tested in patients with Parkinson's disease and healthy controls. Participants were assessed with the Movement Disorder Society Unified Parkinson's disease Rating Scale (MDS-UPDRS) and standardized clinical side effect rating scales, i.e., UKU Side Effect Rating Scale and Simpson-Angus Scale. Additionally, tests of information processing (Symbol Coding Task) and motor speed (Token Motor Task), together with a questionnaire, were applied.

Results:Thirty patients with Parkinson's disease and 33 healthy controls were assessed. In the patient group, there was a statistically significant (p< 0.05) association between prolonged time of motor performance in theMotorgameand upper body rigidity and bradykinesia (MDS-UPDRS) with the strongest effects in the right hand (p< 0.001). In the entire group, prolonged time of motor performance was significantly associated with higher Simson-Angus scale rigidity score and higher UKU hypokinesia scores (p< 0.05). A shortened time of motor performance was significantly associated with higher scores on information processing (p< 0.05). Time of motor performance was not significantly associated with Token Motor Task, duration of illness, or hours of daily physical activity. TheMotorgamewas well-accepted.

Conclusions:In the present feasibility study theMotorgamewas able to detect common motor symptoms in Parkinson's disease in a statistically significant and clinically meaningful way, making it applicable for further testing in larger samples.
Effect of Oxygen Extraction (Brush-Sign) on Baseline Core Infarct Depends on Collaterals (HIR)
Authors: Guenego Adrien , Leipzig Matthew , Fahed Robert , Sussman Eric S. , Faizy Tobias D. , Martin Blake W. , Marcellus David G. , Wintermark Max , Olivot Jean-Marc , Albers Gregory W. , Lansberg Maarten G. , Heit Jeremy J.
Published: January 2021

Objectives: Baseline-core-infarct volume is a critical factor in patient selection and outcome in acute ischemic stroke (AIS) before mechanical thrombectomy (MT). We determined whether oxygen extraction efficiency and arterial collaterals, two different physiologic components of the cerebral ischemic cascade, interacted to modulate baseline-core-infarct volume in patients with AIS-LVO undergoing MT triage.

Methods: Between January 2015 and March 2018, consecutive patients with an AIS and M1 occlusion considered for MT with a baseline MRI and perfusion-imaging were included. Variables such as baseline-core-infarct volume [mL], arterial collaterals (HIR: TMax > 10 s volume/TMax > 6 s), high oxygen extraction (HOE, presence of the brush-sign on T2*) were assessed. A linear-regression was used to test the interaction of HOE and HIR with baseline-core-infarct volume, after including potential confounding variables.

Results: We included 103 patients. Median age was 70 (58–78), and 63% were female. Median baseline-core-infarct volume was 32 ml (IQR 8–74.5). Seventy six patients (74%) had HOE. In a multivariate analysis both favorable HIR collaterals (p = 0.02) and HOE (p = 0.038) were associated with lower baseline-core-infarct volume. However, HOE significantly interacted with HIR (p = 0.01) to predict baseline-core-infarct volume, favorable collaterals (low HIR) with HOE was associated with small baseline-core-infarct whereas patients with poor collaterals (high HIR) and HOE had large baseline-core-infarct.

Conclusion: While HOE under effective collateral blood-flow has the lowest baseline-core-infarct volume of all patients, the protective effect of HOE reverses under poor collateral blood-flow and may be a maladaptive response to ischemic stroke as measured by core infarctions in AIS-LVO patients undergoing MT triage.
Decoding Three Different Preference Levels of Consumers Using Convolutional Neural Network: A Functional Near-Infrared Spectroscopy Study
Authors: Qing Kunqiang , Huang Ruisen , Hong Keum-Shik
Published: January 2021

This study decodes consumers' preference levels using a convolutional neural network (CNN) in neuromarketing. The classification accuracy in neuromarketing is a critical factor in evaluating the intentions of the consumers. Functional near-infrared spectroscopy (fNIRS) is utilized as a neuroimaging modality to measure the cerebral hemodynamic responses. In this study, a specific decoding structure, called CNN-based fNIRS-data analysis, was designed to achieve a high classification accuracy. Compared to other methods, the automated characteristics, constant training of the dataset, and learning efficiency of the proposed method are the main advantages. The experimental procedure required eight healthy participants (four female and four male) to view commercial advertisement videos of different durations (15, 30, and 60 s). The cerebral hemodynamic responses of the participants were measured. To compare the preference classification performances, CNN was utilized to extract the most common features, including the mean, peak, variance, kurtosis, and skewness. Considering three video durations, the average classification accuracies of 15, 30, and 60 s videos were 84.3, 87.9, and 86.4%, respectively. Among them, the classification accuracy of 87.9% for 30 s videos was the highest. The average classification accuracies of three preferences in females and males were 86.2 and 86.3%, respectively, showing no difference in each group. By comparing the classification performances in three different combinations (like vs. so-so, like vs. dislike, and so-so vs. dislike) between two groups, male participants were observed to have targeted preferences for commercial advertising, and the classification performance 88.4% between “like” vs. “dislike” out of three categories was the highest. Finally, pairwise classification performance are shown as follows: For female, 86.1% (like vs. so-so), 87.4% (like vs. dislike), 85.2% (so-so vs. dislike), and for male 85.7, 88.4, 85.1%, respectively.
The Interplay Between Chamber Musicians During Two Public Performances of the Same Piece: A Novel Methodology Using the Concept of “Flow”
Authors: Horwitz Eva Bojner , Harmat László , Osika Walter , Theorell Töres
Published: January 2021

The purpose of the study is to explore a new research methodology that will improve our understanding of “flow” through indicators of physiological and qualitative state. We examine indicators of “flow” experienced by musicians of a youth string quartet, two women (25, 29) and two men (23, 24). Electrocardiogram (ECG) equipment was used to record heart rate variability (HRV) data throughout the four movements in one and the same quartet performed during two concerts. Individual physiological indicators of flow were supplemented by assessments of group “state flow” (means from standardized questionnaires) and a group interview in which the musicians provided qualitative data. A matrix was constructed for the characterization of different kinds of demands in the written music in each one of the four movements for each one of the musicians. HRV derived from ECG data showed non-significant trends for group state flow across the eight musical episodes. Individual-level analysis showed that compared to the other players the first violin player had the highest mean heart rate and the lowest increase in high frequency (HF) power in HRV during this particular movement, particularly during the second concert. The qualitative data illustrated how an interplay of synchronized social interactions between this player and their colleagues during the musical performance was associated with a feeling of group state flow and served to support the first violinist. The case illustrates that the proposed mixed methodology drawing on physiological and qualitative data, has the potential to provide meaningful information about experiences of a flow state, both at individual and group levels. Applications in future research are possible.
Salinity, Temperature and Ammonia Acute Stress Response in Seabream (Sparus aurata) Juveniles: A Multidisciplinary Study
Authors: Zarantoniello Matteo , Bortoletti Martina , Olivotto Ike , Ratti Stefano , Poltronieri Carlo , Negrato Elena , Caberlotto Stefano , Radaelli Giuseppe , Bertotto Daniela
Published: January 2021

The present study aimed to investigate the acute response of gilthead seabream (Sparus aurata) juveniles exposed to temperature, salinity and ammonia stress. Radioimmunoassay was used to evaluate cortisol levels, whereas insulin-like growth factors (igf1 and igf2), myostatin (mstn), heat-shock protein 70 (hsp70) and glucocorticoid receptor (gr) gene expression was assessed trough Real-Time PCR. The presence and localization of IGF-I and HSP70 were investigated by immunohistochemistry. In all the stress conditions, a significant increase in cortisol levels was observed reaching higher values in the thermic and chemical stress groups. Regarding fish growth markers, igf1 gene expression was significantly higher only in fish subjected to heat shock stress while, at 60 min, igf2 gene expression was significantly lower in all the stressed groups. Temperature and ammonia changes resulted in a higher mstn gene expression. Molecular analyses on stress response evidenced a time dependent increase in hsp70 gene expression, that was significantly higher at 60 min in fish exposed to heat shock and chemical stress. Furthermore, the same experimental groups were characterized by a significantly higher gr gene expression respect to the control one. Immunostaining for IGF-I and HSP70 antibodies was observed in skin, gills, liver, and digestive system of gilthead seabream juveniles.
Spatial Characteristics of PM2.5 Pollution among Cities and Policy Implication in the Northern Part of the North China Plain
Authors: Wang Yangjun , Li Hongli , Feng Jin , Wang Wu , Liu Ziyi , Huang Ling , Yaluk Elly , Lu Guibin , Manomaiphiboon Kasemsan , Gong Youguo , Traore Dramane , Li Li
Published: January 2021

In the recent decade, the North China Plain (NCP) has been among the region’s most heavily polluted by PM2.5 in China. For the nonattainment cities in the NCP, joint pollution control with related cities is highly needed in addition to the emission controls in their own cities. However, as the basis of decision-making, the spatial characteristics of PM2.5 among these cities are still insufficiently revealed. In this work, the spatial characteristics among all nonattainment cities in the northern part of the North China Plain (NNCP) region were revealed based on data mining technologies including clustering, coefficient of divergence (COD), network correlation model, and terrain and meteorology analysis. The results indicate that PM2.5 pollution of cities with a distance of less than 180 km exhibits homogeneity in the NCP region. Especially, the sub-region, composed of Xinxiang, Hebi, Kaifeng, Zhengzhou, and Jiaozuo, was strongly homogeneous and a strong correlation exists among them. Compared with spring and summer, much stronger correlations of PM2.5 between cities were found in autumn and winter, indicating a strong need for joint prevention and control during these periods. All nonattainment cities in this region were divided into city-clusters, depending on the seasons and pollution levels to further helping to reduce their PM2.5 concentrations effectively. Air stagnation index (ASI) analysis indicates that the strong correlations between cities in autumn were more attributed to the transport impacts than those in winter, even though there were higher PM2.5 concentrations in winter. These results provided an insight into joint prevention and control of pollution in the NCP region.
Prediction of Acoustic Residual Inhibition of Tinnitus Using a Brain-Inspired Spiking Neural Network Model
Authors: Sanders Philip J. , Doborjeh Zohreh G. , Doborjeh Maryam G. , Kasabov Nikola K. , Searchfield Grant D.
Published: January 2021

Auditory Residual Inhibition (ARI) is a temporary suppression of tinnitus that occurs in some people following the presentation of masking sounds. Differences in neural response to ARI stimuli may enable classification of tinnitus and a tailored approach to intervention in the future. In an exploratory study, we investigated the use of a brain-inspired artificial neural network to examine the effects of ARI on electroencephalographic function, as well as the predictive ability of the model. Ten tinnitus patients underwent two auditory stimulation conditions (constant and amplitude modulated broadband noise) at two time points and were then characterised as responders or non-responders, based on whether they experienced ARI or not. Using a spiking neural network model, we evaluated concurrent neural patterns generated across space and time from features of electroencephalographic data, capturing the neural dynamic changes before and after stimulation. Results indicated that the model may be used to predict the effect of auditory stimulation on tinnitus on an individual basis. This approach may aid in the development of predictive models for treatment selection.
BCI Training Effects on Chronic Stroke Correlate with Functional Reorganization in Motor-Related Regions: A Concurrent EEG and fMRI Study
Authors: Yuan Kai , Chen Cheng , Wang Xin , Chu Winnie Chiu-wing , Tong Raymond Kai-yu
Published: January 2021

Brain–computer interface (BCI)-guided robot-assisted training strategy has been increasingly applied to stroke rehabilitation, while few studies have investigated the neuroplasticity change and functional reorganization after intervention from multimodality neuroimaging perspective. The present study aims to investigate the hemodynamic and electrophysical changes induced by BCI training using functional magnetic resonance imaging (fMRI) and electroencephalography (EEG) respectively, as well as the relationship between the neurological changes and motor function improvement. Fourteen chronic stroke subjects received 20 sessions of BCI-guided robot hand training. Simultaneous EEG and fMRI data were acquired before and immediately after the intervention. Seed-based functional connectivity for resting-state fMRI data and effective connectivity analysis for EEG were processed to reveal the neuroplasticity changes and interaction between different brain regions. Moreover, the relationship among motor function improvement, hemodynamic changes, and electrophysical changes derived from the two neuroimaging modalities was also investigated. This work suggested that (a) significant motor function improvement could be obtained after BCI training therapy, (b) training effect significantly correlated with functional connectivity change between ipsilesional M1 (iM1) and contralesional Brodmann area 6 (including premotor area (cPMA) and supplementary motor area (SMA)) derived from fMRI, (c) training effect significantly correlated with information flow change from cPMA to iM1 and strongly correlated with information flow change from SMA to iM1 derived from EEG, and (d) consistency of fMRI and EEG results illustrated by the correlation between functional connectivity change and information flow change. Our study showed changes in the brain after the BCI training therapy from chronic stroke survivors and provided a better understanding of neural mechanisms, especially the interaction among motor-related brain regions during stroke recovery. Besides, our finding demonstrated the feasibility and consistency of combining multiple neuroimaging modalities to investigate the neuroplasticity change.
SURINPARK: Safinamide for Urinary Symptoms in Parkinson’s Disease
Authors: Gómez-López Ana , Sánchez-Sánchez Arantxa , Natera-Villalba Elena , Ros-Castelló Victoria , Beltrán-Corbellini Álvaro , Fanjul-Arbós Samira , Pareés Moreno Isabel , López-Sendon Moreno José Luis , Martínez Castrillo Juan Carlos , Alonso-Canovas Araceli
Published: January 2021

Background: Urinary symptoms are common, disabling and generally unresponsive to treatment in Parkinson´s disease (PD). Safinamide is approved as an add-on therapy to levodopa to improve fluctuations. Methods: Retrospective analysis of electronic records of nondemented PD patients seen consecutively in a Movement Disorders Unit (November 2018–February 2019). All were assessed with Scale for Outcomes in Parkinson’s disease for Autonomic Symptoms-Urinary subscale (SCOPA-AUT-U) by the attending neurologist, and a month afterwards by an independent researcher blinded to treatment and clinical records in a routine clinical practice setting. Clinical variables were compared among patients who were prescribed safinamide (SA+) for the treatment of motor fluctuations and those with different treatment regimes (SA−). Results: From 169 patients screened initially, 54 were excluded due to severe incontinence, absence of urinary symptoms or previous safinamide treatment. Thirty-five patients were included in SA+ and 79 in SA−. Both groups were comparable in terms of clinical variables, except in basal urinary symptoms, with more severity in the SA+ group. In the follow-up assessment, total SCOPA-AUT-U, as well as urgency, incontinence, frequency and nocturia subscales improved significantly in the SA+ group, while the SA− group remained unchanged. Conclusions: Safinamide could be helpful in the improvement of urinary symptoms in PD.
Cranio-Vertebral Junction Triangular Area: Quantification of Brain Stem Compression by Magnetic Resonance Images
Authors: Chang Chih-Chang , Wu Ching-Lan , Tu Tsung-Hsi , Wu Jau-Ching , Chang Hsuan-Kan , Chang Peng-Yuan , Fay Li-Yu , Huang Wen-Cheng , Cheng Henrich
Published: January 2021

(1) Background: Most of the currently used radiological criteria for craniovertebral junction (CVJ) were developed prior to the popularity of magnetic resonance images (MRIs). This study aimed to evaluate the efficacy of a novel triangular area (TA) calculated on MRIs for pathologies at the CVJ. (2) Methods: A total of 702 consecutive patients were enrolled, grouped into three: (a) Those with pathologies at the CVJ (n = 129); (b) those with underlying rheumatoid arthritis (RA) but no CVJ abnormalities (n = 279); and (3) normal (control; n = 294). TA was defined on T2-weighted MRIs by three points: The lowest point of the clivus, the posterior-inferior point of C2, and the most dorsal indentation point at the ventral brain stem. Receiver operating characteristic (ROC) analysis was used to correlate the prognostic value of the TA with myelopathy. Pre- and post-operative TA values were compared for validation. (c) Results: The CVJ-pathology group had the largest mean TA (1.58 ± 0.47 cm2), compared to the RA and control groups (0.96 ± 0.31 and 1.05 ± 0.26, respectively). The ROC analysis calculated the cutoff-point for myelopathy as 1.36 cm2 with the area under the curve at 0.93. Of the 81 surgical patients, the TA was reduced (1.21 ± 0.37 cm2) at two-years post-operation compared to that at pre-operation (1.67 ± 0.51 cm2). Moreover, intra-operative complete reduction of the abnormalities could further decrease the TA to 1.03 ± 0.39 cm2. (4) Conclusions: The TA, a valid measurement to quantify compression at the CVJ and evaluate the efficacy of surgery, averaged 1.05 cm2 in normal patients, and 1.36 cm2 could be a cutoff-point for myelopathy and of clinical significance.
The Role of Cellular Prion Protein in Promoting Stemness and Differentiation in Cancer
Authors: Ryskalin Larisa , Biagioni Francesca , Busceti Carla L. , Giambelluca Maria A. , Morelli Luca , Frati Alessandro , Fornai Francesco
Published: January 2021

Cellular prion protein (PrPC) is seminal to modulate a variety of baseline cell functions to grant homeostasis. The classic role of such a protein was defined as a chaperone-like molecule being able to rescue cell survival. Nonetheless, PrPC also represents the precursor of the deleterious misfolded variant known as scrapie prion protein (PrPSc). This variant is detrimental in a variety of prion disorders. This multi-faceted role of PrP is greatly increased by recent findings showing how PrPC in its folded conformation may foster tumor progression by acting at multiple levels. The present review focuses on such a cancer-promoting effect. The manuscript analyzes recent findings on the occurrence of PrPC in various cancers and discusses the multiple effects, which sustain cancer progression. Within this frame, the effects of PrPC on stemness and differentiation are discussed. A special emphasis is provided on the spreading of PrPC and the epigenetic effects, which are induced in neighboring cells to activate cancer-related genes. These detrimental effects are further discussed in relation to the aberrancy of its physiological and beneficial role on cell homeostasis. A specific paragraph is dedicated to the role of PrPC beyond its effects in the biology of cancer to represent a potential biomarker in the follow up of patients following surgical resection.
Snail Overexpression Alters the microRNA Content of Extracellular Vesicles Released from HT29 Colorectal Cancer Cells and Activates Pro-Inflammatory State In Vivo
Authors: Papiewska-Pająk Izabela , Przygodzka Patrycja , Krzyżanowski Damian , Soboska Kamila , Szulc-Kiełbik Izabela , Stasikowska-Kanicka Olga , Boncela Joanna , Wągrowska-Danilewicz Małgorzata , Kowalska M. Anna
Published: January 2021

During metastasis, cancer cells undergo phenotype changes in the epithelial-mesenchymal transition (EMT) process. Extracellular vesicles (EVs) released by cancer cells are the mediators of intercellular communication and play a role in metastatic process. Knowledge of factors that influence the modifications of the pre-metastatic niche for the migrating carcinoma cells is important for prevention of metastasis. We focus here on how cancer progression is affected by EVs released from either epithelial-like HT29-cells or from cells that are in early EMT stage triggered by Snail transcription factor (HT29-Snail). We found that EVs released from HT29-Snail, as compared to HT29-pcDNA cells, have a different microRNA profile. We observed the presence of interstitial pneumonias in the lungs of mice injected with HT29-Snail cells and the percent of mice with lung inflammation was higher after injection of HT29-Snail-EVs. Incorporation of EVs released from HT29-pcDNA, but not released from HT29-Snail, leads to the increased secretion of IL-8 from macrophages. We conclude that Snail modifications of CRC cells towards more invasive phenotype also alter the microRNA cargo of released EVs. The content of cell-released EVs may serve as a biomarker that denotes the stage of CRC and EVs-specific microRNAs may be a target to prevent cancer progression.
Immunotherapy as a Precision Medicine Tool for the Treatment of Prostate Cancer
Authors: Adamaki Maria , Zoumpourlis Vassilios
Published: January 2021

Prostate cancer (PCa) is the most frequently diagnosed type of cancer among Caucasian males over the age of 60 and is characterized by remarkable heterogeneity and clinical behavior, ranging from decades of indolence to highly lethal disease. Despite the significant progress in PCa systemic therapy, therapeutic response is usually transient, and invasive disease is associated with high mortality rates. Immunotherapy has emerged as an efficacious and non-toxic treatment alternative that perfectly fits the rationale of precision medicine, as it aims to treat patients on the basis of patient-specific, immune-targeted molecular traits, so as to achieve the maximum clinical benefit. Antibodies acting as immune checkpoint inhibitors and vaccines entailing tumor-specific antigens seem to be the most promising immunotherapeutic strategies in offering a significant survival advantage. Even though patients with localized disease and favorable prognostic characteristics seem to be the ones that markedly benefit from such interventions, there is substantial evidence to suggest that the survival benefit may also be extended to patients with more advanced disease. The identification of biomarkers that can be immunologically targeted in patients with disease progression is potentially amenable in this process and in achieving significant advances in the decision for precision treatment of PCa.
SLC35F2, a Transporter Sporadically Mutated in the Untranslated Region, Promotes Growth, Migration, and Invasion of Bladder Cancer Cells
Authors: Kotolloshi Roland , Hölzer Martin , Gajda Mieczyslaw , Grimm Marc-Oliver , Steinbach Daniel
Published: January 2021

Bladder cancer is a very heterogeneous disease and the molecular mechanisms of carcinogenesis and progression are insufficiently investigated. From the DNA sequencing analysis of matched non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC) samples from eight patients, we identified the tumour-associated gene SLC35F2 to be mutated in the 5′ and 3′ untranslated region (UTR). One mutation in 3′UTR increased the luciferase activity reporter, suggesting its influence on the protein expression of SLC35F2. The mRNA level of SLC35F2 was increased in MIBC compared with NMIBC. Furthermore, in immunohistochemical staining, we observed a strong intensity of SLC35F2 in single tumour cells and in the border cells of solid tumour areas with an atypical accumulation around the nucleus, especially in the MIBC. This suggests that SLC35F2 might be highly expressed in aggressive and invasive tumour cells. Moreover, knockdown of SLC35F2 repressed the growth of bladder cancer cells in the monolayer and spheroid model and suppressed migration and invasion of bladder cancer cells. In conclusion, we suggest that SLC35F2 is involved in bladder cancer progression and might provide a new therapeutic approach, for example, by the anti-cancer drug YM155, a cargo of the SLC35F2 transporter.
Numerical Insights into the Influence of Electrical Properties of n-CdS Buffer Layer on the Performance of SLG/Mo/p-Absorber/n-CdS/n-ZnO/Ag Configured Thin Film Photovoltaic Devices
Authors: Najm Asmaa Soheil , Chelvanathan Puvaneswaran , Tiong Sieh Kiong , Ferdaous Mohammad Tanvirul , Shahahmadi Seyed Ahmad , Yusoff Yulisa , Sopian Kamaruzzaman , Amin Nowshad
Published: January 2021

A CdS thin film buffer layer has been widely used as conventional n-type heterojunction partner both in established and emerging thin film photovoltaic devices. In this study, we perform numerical simulation to elucidate the influence of electrical properties of the CdS buffer layer, essentially in terms of carrier mobility and carrier concentration on the performance of SLG/Mo/p-Absorber/n-CdS/n-ZnO/Ag configured thin film photovoltaic devices, by using the Solar Cell Capacitance Simulator (SCAPS-1D). A wide range of p-type absorber layers with a band gap from 0.9 to 1.7 eV and electron affinity from 3.7 to 4.7 eV have been considered in this simulation study. For an ideal absorber layer (no defect), the carrier mobility and carrier concentration of CdS buffer layer do not significantly alter the maximum attainable efficiency. Generally, it was revealed that for an absorber layer with a conduction band offset (CBO) that is more than 0.3 eV, Jsc is strongly dependent on the carrier mobility and carrier concentration of the CdS buffer layer, whereas Voc is predominantly dependent on the back contact barrier height. However, as the bulk defect density of the absorber layer is increased from 1014 to 1018 cm−3, a CdS buffer layer with higher carrier mobility and carrier concentration is an imperative requirement to a yield device with higher conversion efficiency and a larger band gap-CBO window for realization of a functional device. Most tellingly, simulation outcomes from this study reveal that electrical properties of the CdS buffer layer play a decisive role in determining the progress of emerging p-type photo-absorber layer materials, particularly during the embryonic device development stage.
Determining Interface Fracture Toughness in Multi Layered Environmental Barrier Coatings with Laser Textured Silicon Bond Coat
Authors: Wolf Markus , Kakisawa Hideki , Süß Fabia , Mack Daniel Emil , Vaßen Robert
Published: January 2021

In the high temperature combustion atmosphere inside of aircraft turbines, the currently used ceramic matrix composites require a protective environmental barrier coating (EBC) to mitigate corrosion of the turbine parts. Besides thermomechanical and thermochemical properties like matching thermal expansion coefficient (CTE) and a high resistance against corrosive media, mechanical properties like a high adhesion strength are also necessary for a long lifetime of the EBC. In the present work, the adhesion between an air plasma sprayed silicon bond coat and a vacuum plasma sprayed ytterbium disilicate topcoat was aimed to be enhanced by a laser surface structuring of the Si bond coat. An increase in interface toughness was assumed, since the introduction of structures would lead to an increased mechanical interlocking at the rougher bond coat interface. The interface toughness was measured by a new testing method, which allows the testing of specific interfaces. The results demonstrate a clear increase of the toughness from an original bond coat/topcoat interface (8.6 J/m2) compared to a laser structured interface (14.7 J/m2). Observations in the crack propagation indicates that the laser structuring may have led to a strengthening of the upper bond coat area by sintering. Furthermore, in addition to cohesive failure components, adhesive components can also be observed, which could have influenced the determined toughness.
Description of Echolocation Call Parameters for Urban Bats in Vietnam as a Step Towards a More Integrated Acoustic Monitoring of Urban Wildlife in Southeast Asia
Authors: Pham Long Kim , Tran Bang Van , Le Quy Tan , Nguyen Trung Thanh , Voigt Christian C.
Published: January 2021

This study is the first step towards more systematic monitoring of urban bat fauna in Vietnam and other Southeast Asian countries by collecting bat echolocation call parameters in Ho Chi Minh and Tra Vinh cities. We captured urban bats and then recorded echolocation calls after releasing in a tent. Additional bat’s echolocation calls from the free-flying bats were recorded at the site where we captured bat. We used the obtained echolocation call parameters for a discriminant function analysis to test the accuracy of classifying these species based on their echolocation call parameters. Data from this pilot work revealed a low level of diversity for the studied bat assemblages. Additionally, the discriminant function analysis successfully classified bats to four bat species with an accuracy of >87.4%. On average, species assignments were correct for all calls from Taphozous melanopogon (100% success rate), for 70% of calls from Pipistrellus javanicus, for 80.8% of calls from Myotis hasseltii and 67.3% of calls from Scotophilus kuhlii. Our study comprises the first quantitative description of echolocation call parameters for urban bats of Vietnam. The success in classifying urban bats based on their echolocation call parameters provides a promising baseline for monitoring the effect of urbanization on bat assemblages in Vietnam and potentially also other Southeast Asian countries.
Algorithm to Generate Trajectories in a Robotic Arm Using an LCD Touch Screen to Help Physically Disabled People
Authors: Quiñonez Yadira , Mejía Jezreel , Zatarain Oscar , Lizarraga Carmen , Peraza Juan , Estrada Rogelio
Published: January 2021

In the last two-decade, robotics has attracted a lot of attention from the biomedical sectors, to help physically disabled people in their quotidian lives. Therefore, the research of robotics applied in the control of an anthropomorphic robotic arm to people assistance and rehabilitation has increased considerably. In this context, robotic control is one of the most important problems and is considered the main part of trajectory planning and motion control. The main solution for robotic control is inverse-kinematics, because it provides the angles of robotic arm joints. However, there are disadvantages in the algorithms presented by several authors because the trajectory calculation needs an optimization process which implies more calculations to generate an optimized trajectory. Moreover, the solutions presented by the authors implied devices where the people are dependent or require help from other people to control these devices. This article proposes an algorithm to calculate an accuracy trajectory in any time of interest using an LCD touch screen to calculate the inverse-kinematics and get the end-point of the gripper; the trajectory is calculated using a novel distribution function proposed which makes an easy way to get fast results to the trajectory planning. The obtained results show improvements to generate a safe and fast trajectory of an anthropomorphic robotic arm using an LCD touch screen allowed calculating short trajectories with minimal fingers moves.
Liquid Air as an Energy Carrier for Liquefied Natural Gas Cold Energy Distribution in Cold Storage Systems
Authors: Che Sanghyun , Kim Juwon , Chang Daejun
Published: January 2021

Liquid air can be employed as a carrier of cold energy obtained from liquefied natural gas (LNG) and surplus electricity. This study evaluates the potential of liquid air as a distributed source with a supply chain for a cold storage system using liquid air. Energy storing and distributing processes are conceptually designed and evaluated considering both the thermodynamic and economic aspects. Further, the proposed supply chain is compared with a conventional NH3/CO2 cascade refrigeration system. The thermodynamic analysis demonstrates that the exergy efficiency and the coefficient of performance of the proposed supply chain are 22% and 0.56, respectively. Economic analysis is based on a life cycle cost (LCC) evaluation. From the economic analysis, the liquid air production cost and the LCC of a liquid air cold storage system (LACS) are estimated to be 40.4 USD/ton and 34.2 MMUSD, respectively. The LCC is reduced by 19% in the LACS compared with the conventional refrigeration system. The proposed supply chain is economically feasible, although its thermodynamic performances are lower than those of the conventional system. The sensitivity analysis indicates that LNG mass flow rate in the air liquefaction system and the cold storage operating time are dominant parameters affecting the economic performance.
Supplements for Smoking-Related Lung Diseases
Authors: Alsharairi Naser A.
Published: January 2021

Supplements for smoking-related lung diseases are considered as nonfood products and thought to improve health. Multivitamins and antioxidants are the most commonly dietary supplements used by cancer and asthma patients. There are currently no clear regulatory guidelines that include dietary supplements and their effect on lung cancer and asthma patients, particularly in smokers. Several countries have taken steps to overcome challenges in regulating dietary supplements in the marketplace. These challenges include inadequate assurance of safety/efficacy, inaccuracy of product labeling, misleading health claims, and lack of analytical techniques for dietary supplements. There is a need to establish standards and regulation of dietary supplement use in patients with lung cancer and asthma. The aim of this entry is to expand knowledge on dietary supplements use and smoking-related lung diseases (lung cancer and asthma).
The Impact of Anomalous Diffusion on Action Potentials in Myelinated Neurons
Authors: Drapaca Corina S.
Published: January 2021

Action potentials in myelinated neurons happen only at specialized locations of the axons known as the nodes of Ranvier. The shapes, timings, and propagation speeds of these action potentials are controlled by biochemical interactions among neurons, glial cells, and the extracellular space. The complexity of brain structure and processes suggests that anomalous diffusion could affect the propagation of action potentials. In this paper, a spatio-temporal fractional cable equation for action potentials propagation in myelinated neurons is proposed. The impact of the ionic anomalous diffusion on the distribution of the membrane potential is investigated using numerical simulations. The results show spatially narrower action potentials at the nodes of Ranvier when using spatial derivatives of the fractional order only and delayed or lack of action potentials when adding a temporal derivative of the fractional order. These findings could reveal the pathological patterns of brain diseases such as epilepsy, multiple sclerosis, and Alzheimer’s disease, which have become more prevalent in the latest years.
The LDLR, APOB, and PCSK9 Variants of Index Patients with Familial Hypercholesterolemia in Russia
Authors: Meshkov Alexey , Ershova Alexandra , Kiseleva Anna , Zotova Evgenia , Sotnikova Evgeniia , Petukhova Anna , Zharikova Anastasia , Malyshev Pavel , Rozhkova Tatyana , Blokhina Anastasia , Limonova Alena , Ramensky Vasily , Divashuk Mikhail , Khasanova Zukhra , Bukaeva Anna , Kurilova Olga , Skirko Olga , Pokrovskaya Maria , Mikova Valeriya , Snigir Ekaterina , Akinshina Alexsandra , Mitrofanov Sergey , Kashtanova Daria , Makarov Valentin , Kukharchuk Valeriy , Boytsov Sergey , Yudin Sergey , Drapkina Oxana
Published: January 2021

Familial hypercholesterolemia (FH) is a common autosomal codominant disorder, characterized by elevated low-density lipoprotein cholesterol levels causing premature atherosclerotic cardiovascular disease. About 2900 variants of LDLR, APOB, and PCSK9 genes potentially associated with FH have been described earlier. Nevertheless, the genetics of FH in a Russian population is poorly understood. The aim of this study is to present data on the spectrum of LDLR, APOB, and PCSK9 gene variants in a cohort of 595 index Russian patients with FH, as well as an additional systematic analysis of the literature for the period of 1995–2020 on LDLR, APOB and PCSK9 gene variants described in Russian patients with FH. We used targeted and whole genome sequencing to search for variants. Accordingly, when combining our novel data and the data of a systematic literature review, we described 224 variants: 187 variants in LDLR, 14 variants in APOB, and 23 variants in PCSK9. A significant proportion of variants, 81 of 224 (36.1%), were not described earlier in FH patients in other populations and may be specific for Russia. Thus, this study significantly supplements knowledge about the spectrum of variants causing FH in Russia and may contribute to a wider implementation of genetic diagnostics in FH patients in Russia.
Multi-Omics Approach Reveals miR-SNPs Affecting Muscle Fatty Acids Profile in Nelore Cattle
Authors: Cardoso Tainã Figueiredo , Coutinho Luiz Lehmann , Bruscadin Jennifer Jessica , da Silva Diniz Wellison Jarles , Petrini Juliana , Andrade Bruno Gabriel Nascimento , de Oliveira Priscila Silva Neubern , Poleti Mirele Daiana , Cesar Aline Silva Mello , da Silveira Juliano Coelho , Chiaratti Marcos Roberto , Zerlotini Adhemar , Mourão Gerson Barreto , de Almeida Regitano Luciana Correia
Published: January 2021

MicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration of single nucleotide polymorphisms (SNP) and miRNAs can explain individual differences in economic traits. To provide new insights into the effects of SNPs on miRNAs and their related target genes, we carried out a multi-omic analysis to identify SNPs in miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition in the Nelore cattle. As a result, we identified 3 miR-SNPs in different miRNAs (bta-miR-2419-3p, bta-miR-193a-2, and bta-miR-1291) significantly associated with FA traits (p-value < 0.02, Bonferroni corrected). Among these, the rs110817643C>T, located in the seed sequence of the bta-miR-1291, was associated with different ω6 FAs, polyunsaturated FA, and polyunsaturated:saturated FA ratios. Concerning the other two miR-SNPs, the rs43400521T>C (located in the bta-miR-2419-3p) was associated with C12:0 and C18:1 cis-11 FA, whereas the rs516857374A>G (located in the bta-miR-193a-2) was associated with C18:3 ω6 and ratio of ω6/ω3 traits. Additionally, to identify potential biomarkers for FA composition, we described target genes affected by these miR-SNPs at the mRNA or protein level. Our multi-omics analysis outlines the effects of genetic polymorphism on miRNA, and it highlights miR-SNPs and target candidate genes that control beef fatty acid composition.