bluuebunny/crossref_metadata_2025 huggingface dataset:
THE TIME-SPACES OF CAPITALISM: SUZANNE DE BRUNHOFF AND MONETARY THOUGHT AFTER MARX
Authors: Baronian Laurent
Published: June 2021

The paper is dedicated to Suzanne de Brunhoff’s monetary thought and shows how her analysis of very concrete monetary and financial problems of her time led her to develop the most innovative contributions to Marxist theory of money since classical Marxism. Concepts such as non-contemporaneity of capitalism with itself, pseudo-social validation, conflict centralization, or State management of money and labor power reflect her profound analysis of the ways capitalism generates very particular relations to space and time. By looking at this spatio-temporal dimension of de Brunhoff’s concepts, this paper aims to reveal the novelty, power, and fruitfulness of her monetary analysis. The first part of the paper seeks to define the meaning of the concept of general equivalent, drawing on her reading of Karl Marx’s Capital, before situating her approach in relation to institutionalist theories of money. The second part considers de Brunhoff’s analysis of the particular time-spaces of capitalism.
Chorus and Hiss Scales in the Inner Magnetosphere: Statistics From High‐Resolution Filter Bank (FBK) Van Allen Proves Multi‐Point Measurements
Authors: Agapitov O. , Mourenas D. , Artemyev A. , Breneman A. , Bonnell J. W. , Hospodarsky G. , Wygant J.
Published: June 2021

The spatial scales of whistler‐mode waves, determined by their generation process, propagation, and damping, are important for assessing the scaling and efficiency of wave‐particle interactions affecting the dynamics of the radiation belts. We use multi‐point wave measurements by two Van Allen Probes in 2013–2019 covering all MLTs at L = 2–6 to investigate the spatial extent of active regions of chorus and hiss waves, their wave amplitude distribution in the source/generation region, and the scales of chorus wave packets, employing a time‐domain correlation technique to the spacecraft approaches closer than 1,000 km, which happened every 70 days in 2012–2018 and every 35 days in 2018–2019. The correlation of chorus wave power dynamics using is found to remain significant up to inter‐spacecraft separations of 400–750 km transverse to the background magnetic field direction, consistent with previous estimates of the chorus wave packet extent. Our results further suggest that the chorus source region can be slightly asymmetrical, more elongated in either the azimuthal or radial direction, which could also explain the aforementioned two different scales. An analysis of average chorus and hiss wave amplitudes at separate locations similarly shows the reveals different radial and azimuthal extents of the corresponding wave active regions, complementing previous results based on THEMIS spacecraft statistics mainly at larger L > 6. Both the chorus source region scale and the chorus active region size appear smaller inside the outer radiation belt (at L < 6) than at higher L‐shells.
Overcoming the Model‐Data‐Fit Problem in Porous Media: A Quantitative Method to Compare Invasion‐Percolation Models to High‐Resolution Data
Authors: Banerjee I. , Guthke A. , Van De Ven C. J. C. , Mumford K. G. , Nowak W.
Published: June 2021

Invasion percolation (IP) models offer a computationally inexpensive way to simulate multiphase flow in porous media, but only very few studies have compared their results to actual laboratory experimental image data. One reason might be the difficulty in quantitative assessment: IP models do not have a notion of experimental time but only have an integer counter for simulation steps that imply a time order. Previous experiments‐to‐model comparison studies have either used perceptual similarity or spatial moments as measures of comparison. In this work, we present an objective and quantitative comparison method that overcomes the limitations of the traditional approaches. First, we perform a volume‐based time matching between real‐time experiments and IP model results. Then, we evaluate the quality of fit using a diffused version of the so‐called Jaccard coefficient, which is known from image recognition. We demonstrate our method's applicability on a laboratory‐scale experimental video of gas injection in homogeneous, saturated sand, comparing it to a Macro‐IP model's simulation results. We consider random realizations of the initial entry pressure field to capture the sand's inherent pore‐scale heterogeneity. We find that our proposed method is reliable and intuitive in identifying realistic model realizations. The “strictness”of the method can be adjusted to relevant scales of interest via the blurring (diffusion) radius of the compared images. Beyond the application presented here, our comparison method can be used to compare any high‐resolution space‐time model output to experimental data given as raster images, thus providing valuable insights for model development in many research areas.
Avalanches and edge-of-chaos learning in neuromorphic nanowire networks
Authors: Hochstetter Joel , Zhu Ruomin , Loeffler Alon , Diaz-Alvarez Adrian , Nakayama Tomonobu , Kuncic Zdenka
Published: June 2021

The brain’s efficient information processing is enabled by the interplay between its neuro-synaptic elements and complex network structure. This work reports on the neuromorphic dynamics of nanowire networks (NWNs), a unique brain-inspired system with synapse-like memristive junctions embedded within a recurrent neural network-like structure. Simulation and experiment elucidate how collective memristive switching gives rise to long-range transport pathways, drastically altering the network’s global state via a discontinuous phase transition. The spatio-temporal properties of switching dynamics are found to be consistent with avalanches displaying power-law size and life-time distributions, with exponents obeying the crackling noise relationship, thus satisfying criteria for criticality, as observed in cortical neuronal cultures. Furthermore, NWNs adaptively respond to time varying stimuli, exhibiting diverse dynamics tunable from order to chaos. Dynamical states at the edge-of-chaos are found to optimise information processing for increasingly complex learning tasks. Overall, these results reveal a rich repertoire of emergent, collective neural-like dynamics in NWNs, thus demonstrating the potential for a neuromorphic advantage in information processing.
Impaired reach-to-grasp kinematics in parkinsonian patients relates to dopamine-dependent, subthalamic beta bursts
Authors: Vissani Matteo , Palmisano Chiara , Volkmann Jens , Pezzoli Gianni , Micera Silvestro , Isaias Ioannis U. , Mazzoni Alberto
Published: June 2021

Excessive beta-band oscillations in the subthalamic nucleus are key neural features of Parkinson’s disease. Yet the distinctive contributions of beta low and high bands, their dependency on striatal dopamine, and their correlates with movement kinematics are unclear. Here, we show that the movement phases of the reach-to-grasp motor task are coded by the subthalamic bursting activity in a maximally-informative beta high range. A strong, three-fold correlation linked beta high range bursts, imbalanced inter-hemispheric striatal dopaminergic tone, and impaired inter-joint movement coordination. These results provide new insight into the neural correlates of motor control in parkinsonian patients, paving the way for more informative use of beta-band features for adaptive deep brain stimulation devices.
Endocrine and molecular milieus of ovarian follicles are diversely affected by human chorionic gonadotropin and gonadotropin-releasing hormone in prepubertal and mature gilts
Authors: Ziecik Adam J. , Klos Jan , Gromadzka-Hliwa Katarzyna , Dietrich Mariola A. , Slowinska Mariola , Likszo Pawel , Knapczyk-Stwora Katarzyna , Gajewski Zdzislaw , Kaczmarek Monika M.
Published: June 2021

Different strategies are used to meet optimal reproductive performance or manage reproductive health. Although exogenous human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH) agonists (A) are commonly used to trigger ovulation in estrous cycle synchronization, little is known about their effect on the ovarian follicle. Here, we explored whether hCG- and GnRH-A-induced native luteinizing hormone (LH) can affect the endocrine and molecular milieus of ovarian preovulatory follicles in pigs at different stages of sexual development. We collected ovaries 30 h after hCG/GnRH-A administration from altrenogest and pregnant mare serum gonadotropin (eCG)-primed prepubertal and sexually mature gilts. Several endocrine and molecular alternations were indicated, including broad hormonal trigger-induced changes in follicular fluid steroid hormones and prostaglandin levels. However, sexual maturity affected only estradiol levels. Trigger- and/or maturity-dependent changes in the abundance of hormone receptors (FSHR and LHCGR) and proteins associated with lipid metabolism and steroidogenesis (e.g., STAR, HSD3B1, and CYP11A1), prostaglandin synthesis (PTGS2 and PTGFS), extracellular matrix remodeling (MMP1 and TIMP1), protein folding (HSPs), molecular transport (TF), and cell function and survival (e.g., VIM) were observed. These data revealed different endocrine properties of exogenous and endogenous gonadotropins, with a potent progestational/androgenic role of hCG and estrogenic/pro-developmental function of LH.
Haptic awareness changes when lying down
Authors: Unwalla Kaian , Cadieux Michelle L. , Shore David I.
Published: June 2021

Accurate localization of touch requires the integration of two reference frames—an internal (e.g., anatomical) and an external (e.g., spatial). Using a tactile temporal order judgement task with the hands crossed over the midline, we investigated the integration of these two reference frames. We manipulated the reliability of the visual and vestibular information, both of which contribute to the external reference frame. Visual information was manipulated between experiments (Experiment 1 was done with full vision and Experiment 2 was done while wearing a blindfold). Vestibular information was manipulated in both experiments by having the two groups of participants complete the task in both an upright posture and one where they were lying down on their side. Using a Bayesian hierarchical model, we estimated the perceptual weight applied to these reference frames. Lying participants on their side reduced the weight applied to the external reference frame and produced a smaller deficit; blindfolding resulted in similar reductions. These findings reinforce the importance of the visual system when weighting tactile reference frames, and highlight the importance of the vestibular system in this integration.
Assessment of image generation by quantum annealer
Authors: Sato Takehito , Ohzeki Masayuki , Tanaka Kazuyuki
Published: June 2021

Quantum annealing was originally proposed as an approach for solving combinatorial optimization problems using quantum effects. D-Wave Systems has released a production model of quantum annealing hardware. However, the inherent noise and various environmental factors in the hardware hamper the determination of optimal solutions. In addition, the freezing effect in regions with weak quantum fluctuations generates outputs approximately following a Gibbs–Boltzmann distribution at an extremely low temperature. Thus, a quantum annealer may also serve as a fast sampler for the Ising spin-glass problem, and several studies have investigated Boltzmann machine learning using a quantum annealer. Previous developments have focused on comparing the performance in the standard distance of the resulting distributions between conventional methods in classical computers and sampling by a quantum annealer. In this study, we focused on the performance of a quantum annealer as a generative model from a different aspect. To evaluate its performance, we prepared a discriminator given by a neural network trained on an a priori dataset. The evaluation results show a higher performance of quantum annealer compared with the classical approach for Boltzmann machine learning in training of the generative model. However the generation of the data suffers from the remanent quantum fluctuation in the quantum annealer. The quality of the generated images from the quantum annealer gets worse than the ideal case of the quantum annealing and the classical Monte-Carlo sampling.
Functionally significant polymorphisms of the MMP-9 gene are associated with peptic ulcer disease in the Caucasian population of Central Russia
Authors: Minyaylo Oksana , Ponomarenko Irina , Reshetnikov Evgeny , Dvornyk Volodymyr , Churnosov Mikhail
Published: June 2021

This study analyzed the association of functionally significant SNPs of matrix metalloproteinase (MMP) genes in the development of peptic ulcer disease (PUD) in Caucasians from Central Russia. Ten SNPs of theMMP-1, MMP-2, MMP-3, MMP-8, andMMP-9genes were analyzed for association with PUD in a cohort of 798 patients with PUD (including 404H. pylori-positive and 394H. pylori-negative) and 347H. pylori-negative controls using logistic regression and assuming the additive, recessive, and dominant genetic models. The variants ofMMP-1,MMP-2,MMP-3, andMMP-8did not manifest any significant associations with the diseases. Five SNPs of theMMP-9gene demonstrated such association. Allele G of the rs17576MMP-9locus conferred a higher risk for PUD (ORadj = 1.31, pperm = 0.016), haplotype AACG of loci rs17576-rs3787268-rs2250889-rs17577 of theMMP-9gene decreased risk for PUD (ORadj = 0.17, pperm = 0.003). Also, allele C of rs3918249, allele G of rs17576 and haplotype CG of rs3918249-rs17576 of theMMP-9gene increased risk forH. pylori-positive PUD (ORadj = 1.82, pperm = 0.002; ORadj = 1.53–1.95 pperm = 0.001–0.013 and ORadj = 1.49 pperm = 0.009 respectively). The above loci and 50 linked to them possess significant regulatory effects and may affect the alternative splicing of four genes and the expression of 17 genes in various organs and tissues related to the PUD pathogenesis.
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs
Authors: Smolinska Agnieszka , Jessop David S. , Pappan Kirk L. , De Saedeleer Alexandra , Kang Amerjit , Martin Alexandra L. , Allsworth Max , Tyson Charlotte , Bos Martine P. , Clancy Matt , Morel Mike , Cooke Tony , Dymond Tom , Harris Claire , Galloway Jacqui , Bresser Paul , Dijkstra Nynke , Jagesar Viresh , Savelkoul Paul H. M. , Beuken Erik V. H. , Nix Wesley H. V. , Louis Renaud , Delvaux Muriel , Calmes Doriane , Ernst Benoit , Pollini Simona , Peired Anna , Guiot Julien , Tomassetti Sara , Budding Andries E. , McCaughan Frank , Marciniak Stefan J. , van der Schee Marc P.
Published: June 2021

Face masks and personal respirators are used to curb the transmission of SARS-CoV-2 in respiratory droplets; filters embedded in some personal protective equipment could be used as a non-invasive sample source for applications, including at-home testing, but information is needed about whether filters are suited to capture viral particles for SARS-CoV-2 detection. In this study, we generated inactivated virus-laden aerosols of 0.3–2 microns in diameter (0.9 µm mean diameter by mass) and dispersed the aerosolized viral particles onto electrostatic face mask filters. The limit of detection for inactivated coronaviruses SARS-CoV-2 and HCoV-NL63 extracted from filters was between 10 to 100 copies/filter for both viruses. Testing for SARS-CoV-2, using face mask filters and nasopharyngeal swabs collected from hospitalized COVID-19-patients, showed that filter samples offered reduced sensitivity (8.5% compared to nasopharyngeal swabs). The low concordance of SARS-CoV-2 detection between filters and nasopharyngeal swabs indicated that number of viral particles collected on the face mask filter was below the limit of detection for all patients but those with the highest viral loads. This indicated face masks are unsuitable to replace diagnostic nasopharyngeal swabs in COVID-19 diagnosis. The ability to detect nucleic acids on face mask filters may, however, find other uses worth future investigation.
The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
Authors: Oliveira Cleide , Valois Mayara Vioto , Ottaiano Tatiana Fontes , Miranda Antonio , Hansen Daiane , Sampaio Misako Uemura , Oliva Maria Luiza Vilela , de Abreu Maffei Francisco Humberto
Published: June 2021

The anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His(6) (containing a histidine tail) in an experimental venous thrombosis model of vena cava (VC) ligature in rats, comparing to heparin. We evaluate the effects of the inhibitors (native or recombinant) or heparin on the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in human and rat plasmas. The rats undergoing treatment received a saline solution or increasing concentrations of rBbCI-His(6), heparin, or a mixture of both. After 4 h of ligature VC, thrombus, if present was removed and weighed. aPTT, PT, and cytokines were measured in blood collected by cardiac puncture. aPTT, PT, and bleeding time (BT) were also measured at the time of VC (vena cava) ligature. rBbCI-His(6) (0.45 or 1.40 mg/kg) does not alter aPTT, PT or BT. No differences in coagulation parameters were detected in rBbCI-His(6) treated rats at the time of VC ligature or when the thrombus was removed. There was a significant decrease in the weight of thrombus in the animals of the groups treated with the rBbCI-His(6) (1.40 mg/kg), with the rBbCI-His(6) mixture (1.40 mg/kg) + heparin (50 IU/kg) and heparin (100 IU/kg) in relation to control group (saline). The growth-related oncogene/keratinocyte chemoattractant (GRO/KC) serum levels in rats treated with rBbCI-His(6) (1.40 mg/kg) or heparin (200 IU/kg) were reduced. In the experimental model used, rBbCI-His(6) alone had an antithrombotic effect, not altering blood clotting or bleeding time.
The risk of major bleeding event in patients with chronic kidney disease on pentoxifylline treatment
Authors: Fang Jing-Hung , Chen Yi-Chen , Ho Chung-Han , Chen Jui-Yi , Hsing Chung-Hsi , Liang Fu-Wen , Wu Chia-Chun
Published: June 2021

Patients with chronic kidney diseases (CKD) are often treated with antiplatelets due to aberrant haemostasis. This study aimed to evaluate the bleeding risk with CKD patients undergoing pentoxifylline (PTX) treatment with/without aspirin. In this retrospective study, we used Taiwan’s National Health Insurance Research Database to identify PTX treated CKD patients. Patients undergoing PTX treatment after CKD diagnosis were PTX group. A 1:4 age, sex and aspirin used condition matched CKD patients non-using PTX were identified as controls. The outcome was major bleeding event (MBE: intracranial haemorrhage (ICH) and gastrointestinal tract bleeding) during 2-year follow-up period. Risk factors were estimated using Cox regression for overall and stratified analysis. The PTX group had higher MBE risk than controls (hazard ratio (HR) 1.19; 95% confidence interval (CI) 0.94–1.50). In stratified analysis, hyperlipidaemia was a significant risk factor (HR: 1.42; 95% CI 1.01–2.01) of MBE. A daily PTX dose larger than 800 mg, females, non-regular aspirin usage, and ischaemic stroke were risk factors for MBE in PTX group. When prescribing PTX in CKD patients, bleeding should be closely monitored, especially in those with daily dose more than 800 mg, aspirin users, and with a history of ischaemic stroke.
Evolution, heterogeneity and global dispersal of cosmopolitan genotype of Dengue virus type 2
Authors: Yenamandra Surya Pavan , Koo Carmen , Chiang Suzanna , Lim Han Shi Jeri , Yeo Zhen Yuan , Ng Lee Ching , Hapuarachchi Hapuarachchige Chanditha
Published: June 2021

Dengue virus type 2 (DENV-2) contributes substantially to the dengue burden and dengue-related mortality in the tropics and sub-tropics. DENV-2 includes six genotypes, among which cosmopolitan genotype is the most widespread. The present study investigated the evolution, intra-genotype heterogeneity and dispersal of cosmopolitan genotype to understand unique genetic characteristics that have shaped the molecular epidemiology and distribution of cosmopolitan lineages. The spatial analysis demonstrated a wide geo-distribution of cosmopolitan genotype through an extensive inter-continental network, anchored in Southeast Asia and Indian sub-continent. Intra-genotype analyses using 3367 envelope gene sequences revealed six distinct lineages within the cosmopolitan genotype, namely the Indian sub-continent lineage and five other lineages. Indian sub-continent lineage was the most diverged among six lineages and has almost reached the nucleotide divergence threshold of 6% within E gene to qualify as a separate genotype. Genome wide amino acid signatures and selection pressure analyses further suggested differences in evolutionary characteristics between the Indian sub-continent lineage and other lineages. The present study narrates a comprehensive genomic analysis of cosmopolitan genotype and presents notable genetic characteristics that occurred during its evolution and global expansion. Whether those characteristics conferred a fitness advantage to cosmopolitan genotype in different geographies warrant further investigations.
Identification of small compounds regulating the secretion of extracellular vesicles via a TIM4-affinity ELISA
Authors: Ma Yunfei , Yoshida Takeshi , Matoba Kazutaka , Kida Katsuhiko , Shintani Rito , Piao Yingshi , Jin Jingchun , Nishino Taito , Hanayama Rikinari
Published: June 2021

Extracellular vesicles (EVs) are secreted from most cells and play important roles in cell–cell communication by transporting proteins, lipids, and nucleic acids. As the involvement of EVs in diseases has become apparent, druggable regulators of EV secretion are required. However, the lack of a highly sensitive EV detection system has made the development of EV regulators difficult. We developed an ELISA system using a high-affinity phosphatidylserine-binder TIM4 to capture EVs and screened a 1567-compound library. Consequently, we identified one inhibitor and three activators of EV secretion in a variety of cells. The inhibitor, apoptosis activator 2, suppressed EV secretion via a different mechanism and had a broader cellular specificity than GW4869. Moreover, the three activators, namely cucurbitacin B, gossypol, and obatoclax, had broad cellular specificity, including HEK293T cells and human mesenchymal stem cells (hMSCs). In vitro bioactivity assays revealed that some regulators control EV secretion from glioblastoma and hMSCs, which induces angiogenesis and protects cardiomyocytes against apoptosis, respectively. In conclusion, we developed a high-throughput method to detect EVs with high sensitivity and versatility, and identified four compounds that can regulate the bioactivity of EVs.
Concurrent YAP/TAZ and SMAD signaling mediate vocal fold fibrosis
Authors: Nakamura Ryosuke , Hiwatashi Nao , Bing Renjie , Doyle Carina P. , Branski Ryan C.
Published: June 2021

Vocal fold (VF) fibrosis is a major cause of intractable voice-related disability and reduced quality of life. Excision of fibrotic regions is suboptimal and associated with scar recurrence and/or further iatrogenic damage. Non-surgical interventions are limited, putatively related to limited insight regarding biochemical events underlying fibrosis, and downstream, the lack of therapeutic targets. YAP/TAZ integrates diverse cell signaling events and interacts with signaling pathways related to fibrosis, including the TGF-β/SMAD pathway. We investigated the expression of YAP/TAZ following vocal fold injury in vivo as well as the effects of TGF-β1 on YAP/TAZ activity in human vocal fold fibroblasts, fibroblast-myofibroblast transition, and TGF-β/SMAD signaling. Iatrogenic injury increased nuclear localization of YAP and TAZ in fibrotic rat vocal folds. In vitro, TGF-β1 activated YAP and TAZ in human VF fibroblasts, and inhibition of YAP/TAZ reversed TGF-β1-stimulated fibroplastic gene upregulation. Additionally, TGF-β1 induced localization of YAP and TAZ in close proximity to SMAD2/3, and nuclear accumulation of SMAD2/3 was inhibited by a YAP/TAZ inhibitor. Collectively, YAP and TAZ were synergistically activated with the TGF-β/SMAD pathway, and likely essential for the fibroplastic phenotypic shift in VF fibroblasts. Based on these data, YAP/TAZ may evolve as an attractive therapeutic target for VF fibrosis.
Identifying aphid resistance in the ancestral wheat Triticum monococcum under field conditions
Authors: Simon Amma L. , Caulfield John C. , Hammond-Kosack Kim E. , Field Linda M. , Aradottir Gudbjorg I.
Published: June 2021

Wheat is an economically, socially, and nutritionally important crop, however, aphid infestation can often reduce wheat yield through feeding and virus transmission. Through field phenotyping, we investigated aphid resistance in ancestral wheatTriticum monococcum(L.). Aphid (Rhopalosiphum padi(L.), Sitobion avenae(F.) andMetopolophium dirhodum(Wlk.)) populations and natural enemy presence (parasitised mummified aphids, ladybird adults and larvae and lacewing eggs and larvae) on two naturally susceptible wheat varieties,Triticum aestivum(L.) var. Solstice andT. monococcumMDR037, and three potentially resistant genotypesT. monococcumMDR657, MDR045 and MDR049 were monitored across three years of field trials.Triticum monococcumMDR045 and MDR049 had smaller aphid populations, whereas MDR657 showed no resistance. Overall, natural enemy presence was positively correlated with aphid populations; however, MDR049 had similar natural enemy presence to MDR037 which is susceptible to aphid infestation. It is hypothesised that alongside reducing aphid population growth, MDR049 also confers indirect resistance by attracting natural enemies. The observed resistance to aphids in MDR045 and MDR049 has strong potential for introgression into commercial wheat varieties, which could have an important role in Integrated Pest Management strategies to reduce aphid populations and virus transmission.
The reliability and validity of DSM 5 diagnostic criteria for neurocognitive disorder and relationship with plasma neurofilament light in a down syndrome population
Authors: Pape Sarah E. , al Janabi Tamara , Ashton Nicholas J. , Hye Abdul , Sheehan Rory , Gallagher Paul , Knight Bernice , Prins Anne-Marije , Courtenay Ken , Jordanova Vesna , Thomas Bini , Perumal Nagarajan , Forbes Craig , Hassiotis Angela , Strydom Andre
Published: June 2021

The validity of dementia diagnostic criteria depends on their ability to distinguish dementia symptoms from pre-existing cognitive impairments. The study aimed to assess inter-rater reliability and concurrent validity of DSM-5 criteria for neurocognitive disorder in Down syndrome. The utility of mild neurocognitive disorder as a distinct diagnostic category, and the association between clinical symptoms and neurodegenerative changes represented by the plasma biomarker neurofilament light were also examined. 165 adults with Down syndrome were included. Two clinicians independently applied clinical judgement, DSM-IV, ICD-10 and DSM-5 criteria for dementia (or neurocognitive disorder) to each case. Inter-rater reliability and concurrent validity were analysed using the kappa statistic. Plasma neurofilament light concentrations were measured for 55 participants as a marker of neurodegeneration and between group comparisons calculated. All diagnostic criteria showed good inter-rater reliability apart from mild neurocognitive disorder which was moderate (k = 0.494). DSM- 5 criteria had substantial concurrence with clinical judgement (k = 0.855). When compared to the no neurocognitive disorder group, average neurofilament light concentrations were higher in both the mild and major neurocognitive disorder groups. DSM-5 neurocognitive disorder criteria can be used reliably in a Down syndrome population and has higher concurrence with clinical judgement than the older DSM-IV and ICD-10 criteria. Whilst the inter-rater reliability of the mild neurocognitive disorder criteria was modest, it does appear to identify people in an early stage of dementia with underlying neurodegenerative changes, represented by higher average NfL levels.
Uncovering complexity details in actigraphy patterns to differentiate the depressed from the non-depressed
Authors: George Sandip Varkey , Kunkels Yoram K , Booij Sanne , Wichers Marieke
Published: June 2021

While the negative association between physical activity and depression has been well established, it is unclear what precise characteristics of physical activity patterns explain this association. Complexity measures may identify previously unexplored aspects of objectively measured activity patterns, such as the extent to which individuals show repetitive periods of physical activity and the diversity in durations of such repetitive activity patterns. We compared the complexity levels of actigraphy data gathered over 4 weeks ($$\sim 40000$$

∼
40000

data points each) for every individual, from non-depressed ($$n=25$$

n
=
25

) and depressed ($$n=21$$

n
=
21

) groups using recurrence plots. Significantly lower levels of complexity were detected in the actigraphy data from the depressed group as compared to non-depressed controls, both in terms of lower mean durations of periods of recurrent physical activity and less diversity in the duration of these periods. Further, diagnosis of depression was not significantly associated with mean activity levels or measures of circadian rhythm stability, and predicted depression status better than these.
SpO2/FiO2 as a predictor of high flow nasal cannula outcomes in children with acute hypoxemic respiratory failure
Authors: Kim Ga Eun , Choi Sun Ha , Park Mireu , Jung Jae Hwa , Lee Myeongjee , Kim Soo Yeon , Kim Min Jung , Kim Yoon Hee , Kim Kyung Won , Sohn Myung Hyun
Published: June 2021

The high-flow nasal cannula (HFNC) is a useful treatment modality for acute hypoxemic respiratory failure (AHRF) in children. We compared the ability of the oxygen saturation to fraction of inspired oxygen ratio (S/F) and arterial oxygen partial pressure to fraction of inspired oxygen ratio (P/F) to predict HFNC outcomes in children with AHRF. This study included children treated with HFNC due to AHRF from April 2013 to March 2019 at the Severance Children’s Hospital. HFNC failure was defined as the need for mechanical ventilation. Trends of S/F and P/F during HFNC were analyzed. To predict HFNC outcomes, a nomogram was constructed based on predictive factors. A total of 139 patients with arterial blood gas data were included in the S/F and P/F analyses. S/F < 230 at initiation showed high prediction accuracy for HFNC failure (area under the receiver operating characteristic curve: 0.751). Univariate analyses identified S/F < 230 at HFNC initiation and < 200 at 2 h (odds ratio [OR] 12.83, 95% CI 5.06–35.84), and hemato-oncologic disease (OR 3.79, 95% CI 1.12–12.78) as significant predictive factors of HFNC failure. The constructed nomogram had a highly predictive performance, with a concordance index of 0.765 and 0.831 for the exploratory and validation groups, respectively. S/F may be used as a predictor of HFNC outcomes. Our nomogram with S/F for HFNC failure within 2 h may prevent delayed intubation in children with AHRF.
Hangboard training in advanced climbers: A randomized controlled trial
Authors: Mundry Saskia , Steinmetz Gino , Atkinson Elizabeth J. , Schilling Arndt F. , Schöffl Volker R. , Saul Dominik
Published: June 2021

Improving climbing performance strongly depends upon effective training methods. Hangboard training is one of the most popular approaches to increase finger and forearm strength. Training protocols are based on maximizing weight or minimizing edges. We aimed to evaluate which of these protocols was superior. We prospectively analyzed 30 intermediate to advanced climbing athletes [Union Internationale des Associations d'Alpinisme (UIAA) VI–VIII] and randomized them into three groups: control group C (Control, normal climbing training), hangboard group HE (Hang endurance, grips to hold for a determined time decreased every week), and hangboard group HW (Hang weight, + 1.25 kg weight were added each week to hold for a determined time). As endpoints, we measured the grip strength before and after an 8-week training protocol in seven different pinches. Over the 8-week training period, HW hangboard training significantly improved the climbers’ grip strength compared to C [p = 0.032, effect size (ES) 0.36]. Maximizing weight improved the strength in I/II + III, I/II + III + IV and fist significantly. HW was superior compared to C in terms of grip strength improvement in three out of seven pinches and overall grip strength. The overall changes in the HE group did not differ significantly from the C group. An 8-week training protocol with increasing weights (HW) significantly improved overall grip strength more than a regular climbing training without the use of a hangboard.
Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
Authors: Iqbal Muhammad Arsalan , Ali Asghar , Hadlich Frieder , Oster Michael , Reyer Henry , Trakooljul Nares , Sommerfeld Vera , Rodehutscord Markus , Wimmers Klaus , Ponsuksili Siriluck
Published: June 2021

Phosphorus (P) and calcium (Ca) are critical for egg production in laying hens. Most of P in plant-based poultry diet is bound as phytic acid and needs to be hydrolysed before absorption. To increase P bioavailability, exogenous phytases or bioavailable rock phosphate is added in feed. There is growing evidence of the importance of miRNAs as the epicentre of intestinal homeostasis and functional properties. Therefore, we demonstrated the expression of miRNA profiles and the corresponding target genes due to the different levels of P (recommended vs. 20% reduction) and/or Ca (recommended vs. 15% reduction) in feed. Jejunal miRNA profiles of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens strains were used (n = 80). A total of 34 and 76 miRNAs were differentially expressed (DE) in the different diet groups within LSL and LB strains respectively. In LSL, the DE miRNAs and their targets were involved in calcium signaling pathway, inositol phosphate metabolism, and mitochondrial dysfunction. Similarly, in LB miRNAs targets were enriched in metabolic pathways such as glutathione metabolism, phosphonate metabolism and vitamin B6 metabolism. Our results suggest that both strains employ different intrinsic strategies to cope with modulated P and Ca supply and maintain mineral homeostasis.
Unraveling regulatory divergence, heterotic malleability, and allelic imbalance switching in rice due to drought stress
Authors: Ereful Nelzo C. , Laurena Antonio , Liu Li-Yu , Kao Shu-Min , Tsai Eric , Greenland Andy , Powell Wayne , Mackay Ian , Leung Hei
Published: June 2021

The indica ecotypes, IR64, an elite drought-susceptible variety adapted to irrigated ecosystem, and Apo (IR55423-01 or NSIC RC9), a moderate drought-tolerant upland genotype together with their hybrid (IR64 × Apo) were exposed to non- and water-stress conditions. By sequencing (RNA-seq) these genotypes, we were able to map genes diverging in cis and/or trans factors. Under non-stress condition, cis dominantly explains (11.2%) regulatory differences, followed by trans (8.9%). Further analysis showed that water-limiting condition largely affects trans and cis + trans factors. On the molecular level, cis and/or trans regulatory divergence explains their genotypic differences and differential drought response. Between the two parental genotypes, Apo appears to exhibit more photosynthetic efficiency even under water-limiting condition and is ascribed to trans. Statistical analyses showed that regulatory divergence is significantly influenced by environmental conditions. Likewise, the mode of parental expression inheritance which drives heterosis (HET) is significantly affected by environmental conditions indicating the malleability of heterosis to external factors. Further analysis revealed that the HET class, dominance, was significantly enriched under water-stress condition. We also identified allelic imbalance switching in which several genes prefer IR64- (or Apo-) specific allele under non-stress condition but switched to Apo- (or IR64-) specific allele when exposed to water-stress condition.
Impact of proton therapy on antitumor immune response
Authors: Mirjolet Céline , Nicol Anaïs , Limagne Emeric , Mura Carole , Richard Corentin , Morgand Véronique , Rousseau Marc , Boidot Romain , Ghiringhelli François , Noel Georges , Burckel Hélène
Published: June 2021

Radiotherapy delivered using photons induces an immune response that leads to modulation of the tumor microenvironment. Clinical studies are ongoing to evaluate immune checkpoint inhibitors in association with photon radiotherapy. At present, there is no publication on the radio-induced immune response after proton therapy. Balb/c mice bearing subcutaneous CT26 colon tumors were irradiated by a single fraction of 16.4 Gy using a proton beam extracted from a TR24 cyclotron. RNA sequencing analysis was assessed at 3 days post-treatment. Proton therapy immune response was monitored by flow cytometry using several panels (lymphoid, myeloid cells, lymphoid cytokines) at 7 and 14 days post-irradiation. RNA-Seq functional profiling identified a large number of GO categories linked to “immune response” and “interferon signaling”. Immunomonitoring evaluation showed induced tumor infiltration by immune cells. This is the first study showing the effect of proton therapy on immune response. These interesting results provide a sound basis to assess the efficacy of a combination of proton therapy and immune checkpoint inhibitors.
De novo deoxyribonucleotide biosynthesis regulates cell growth and tumor progression in small-cell lung carcinoma
Authors: Maruyama Ami , Sato Yuzo , Nakayama Joji , Murai Junko , Ishikawa Takamasa , Soga Tomoyoshi , Makinoshima Hideki
Published: June 2021

Deoxyribonucleotide biosynthesis from ribonucleotides supports the growth of active cancer cells by producing building blocks for DNA. Although ribonucleotide reductase (RNR) is known to catalyze the rate-limiting step of de novo deoxyribonucleotide triphosphate (dNTP) synthesis, the biological function of the RNR large subunit (RRM1) in small-cell lung carcinoma (SCLC) remains unclear. In this study, we established siRNA-transfected SCLC cell lines to investigate the anticancer effect of silencing RRM1 gene expression. We found that RRM1 is required for the full growth of SCLC cells both in vitro and in vivo. In particular, the deletion of RRM1 induced a DNA damage response in SCLC cells and decreased the number of cells with S phase cell cycle arrest. We also elucidated the overall changes in the metabolic profile of SCLC cells caused by RRM1 deletion. Together, our findings reveal a relationship between the deoxyribonucleotide biosynthesis axis and key metabolic changes in SCLC, which may indicate a possible link between tumor growth and the regulation of deoxyribonucleotide metabolism in SCLC.
Reproducibility of automated habenula segmentation via deep learning in major depressive disorder and normal controls with 7 Tesla MRI
Authors: Lim Sang-Heon , Yoon Jihyun , Kim Young Jae , Kang Chang-Ki , Cho Seo-Eun , Kim Kwang Gi , Kang Seung-Gul
Published: June 2021

The habenula is one of the most important brain regions for investigating the etiology of psychiatric diseases such as major depressive disorder (MDD). However, the habenula is challenging to delineate with the naked human eye in brain imaging due to its low contrast and tiny size, and the manual segmentation results vary greatly depending on the observer. Therefore, there is a great need for automatic quantitative analytic methods of the habenula for psychiatric research purposes. Here we propose an automated segmentation and volume estimation method for the habenula in 7 Tesla magnetic resonance imaging based on a deep learning-based semantic segmentation network. The proposed method, using the data of 69 participants (33 patients with MDD and 36 normal controls), achieved an average precision, recall, and dice similarity coefficient of 0.869, 0.865, and 0.852, respectively, in the automated segmentation task. Moreover, the intra-class correlation coefficient reached 0.870 in the volume estimation task. This study demonstrates that this deep learning-based method can provide accurate and quantitative analytic results of the habenula. By providing rapid and quantitative information on the habenula, we expect our proposed method will aid future psychiatric disease studies.
Genetic diversity of Colletotrichum lupini and its virulence on white and Andean lupin
Authors: Alkemade J. A. , Messmer M. M. , Voegele R. T. , Finckh M. R. , Hohmann P.
Published: June 2021

Lupin cultivation worldwide is threatened by anthracnose, a destructive disease caused by the seed- and air-borne fungal pathogen Colletotrichum lupini. In this study we explored the intraspecific diversity of 39 C. lupini isolates collected from different lupin cultivating regions around the world, and representative isolates were screened for their pathogenicity and virulence on white and Andean lupin. Multi-locus phylogeny and morphological characterizations showed intraspecific diversity to be greater than previously shown, distinguishing a total of six genetic groups and ten distinct morphotypes. Highest diversity was found across South America, indicating it as the center of origin of C. lupini. The isolates that correspond to the current pandemic belong to a genetic and morphological uniform group, were globally widespread, and showed high virulence on tested white and Andean lupin accessions. Isolates belonging to the other five genetic groups were mostly found locally and showed distinct virulence patterns. Two highly virulent strains were shown to overcome resistance of advanced white lupin breeding material. This stresses the need to be careful with international seed transports in order to prevent spread of currently confined but potentially highly virulent strains. This study improves our understanding of the diversity, phylogeography and pathogenicity of a member of one of the world’s top 10 plant pathogen genera, providing valuable information for breeding programs and future disease management.
Younger age of onset and uveitis associated with HLA-B27 and delayed diagnosis in Thai patients with axial spondyloarthritis
Authors: Limsakul Naphruet , Chiowchanwisawakit Praveena , Permpikul Parichart , Thanaketpaisarn Yubolrat
Published: June 2021

To identify characteristics associated with HLA-B27, and to identify factors associated with delayed diagnosis in Thai patients with axial spondyloarthritis (axSpA). This cross-sectional study included Thai patients were diagnosed with axSpA by a rheumatologist at Siriraj Hospital. Clinical data were collected. Regression analyses were employed to identify factors associated with study outcomes. Of total 177 patients, 127 (72%) were positive HLA-B27. Uveitis [Odds ratio (OR) 4.0], age at onset of the first musculoskeletal symptom of ≤ 28 years [OR 3.5], female [OR 0.4], and psoriasis [OR 0.4] were significantly associated with HLA-B27 in multiple regression analysis. Those with positive HLA-B27 had less spinal flexibility. Elevated C-reactive protein (p = 0.012) was associated with shorter delay in diagnosis, while uveitis (p < 0.001) and younger age at onset of the first symptom (p = 0.002) were associated with longer delay in diagnosis in multiple regression analysis. Younger age at onset of the first musculoskeletal symptom and uveitis were associated with HLA-B27 and delayed diagnosis in axSpA patients. Young people with musculoskeletal symptom and uveitis should be referred to a rheumatologist to rule out or make a timely diagnosis of axSpA.
Radiomics is feasible for prediction of spread through air spaces in patients with nonsmall cell lung cancer
Authors: Onozato Yuki , Nakajima Takahiro , Yokota Hajime , Morimoto Jyunichi , Nishiyama Akira , Toyoda Takahide , Inage Terunaga , Tanaka Kazuhisa , Sakairi Yuichi , Suzuki Hidemi , Uno Takashi , Yoshino Ichiro
Published: June 2021

Tumor spread through air spaces (STAS) in non-small-cell lung cancer (NSCLC) is known to influence a poor patient outcome, even in patients presenting with early-stage disease. However, the pre-operative diagnosis of STAS remains challenging. With the progress of radiomics-based analyses several attempts have been made to predict STAS based on radiological findings. In the present study, patients with NSCLC which is located peripherally and tumors ≤ 2 cm in size on computed tomography (CT) that were potential candidates for sublobar resection were enrolled in this study. The radiologic features of the targeted tumors on thin-section CT were extracted using the PyRadiomics v3.0 software package, and a predictive model for STAS was built using the t-test and XGBoost. Thirty-five out of 226 patients had a STAS histology. The predictive model of STAS indicated an area under the receiver-operator characteristic curve (AUC) of 0.77. There was no significant difference in the overall survival (OS) for lobectomy between the predicted-STAS (+) and (−) groups (p = 0.19), but an unfavorable OS for sublobar resection was indicated in the predicted-STAS (+) group (p < 0.01). These results suggest that radiomics with machine-learning helped to develop a favorable model of STAS (+) NSCLC, which might be useful for the proper selection of candidates who should undergo sublobar resection.
Transmission of bee-like vibrations in buzz-pollinated plants with different stamen architectures
Authors: Nevard Lucy , Russell Avery L. , Foord Karl , Vallejo-Marín Mario
Published: June 2021

In buzz-pollinated plants, bees apply thoracic vibrations to the flower, causing pollen release from anthers, often through apical pores. Bees grasp one or more anthers with their mandibles, and vibrations are transmitted to this focal anther(s), adjacent anthers, and the whole flower. Pollen release depends on anther vibration, and thus it should be affected by vibration transmission through flowers with distinct morphologies, as found among buzz-pollinated taxa. We compare vibration transmission between focal and non-focal anthers in four species with contrasting stamen architectures: Cyclamen persicum, Exacum affine, Solanum dulcamara and S. houstonii. We used a mechanical transducer to apply bee-like vibrations to focal anthers, measuring the vibration frequency and displacement amplitude at focal and non-focal anther tips simultaneously using high-speed video analysis (6000 frames per second). In flowers in which anthers are tightly arranged (C. persicum and S. dulcamara), vibrations in focal and non-focal anthers are indistinguishable in both frequency and displacement amplitude. In contrast, flowers with loosely arranged anthers (E. affine) including those with differentiated stamens (heterantherous S. houstonii), show the same frequency but higher displacement amplitude in non-focal anthers compared to focal anthers. We suggest that stamen architecture modulates vibration transmission, potentially affecting pollen release and bee behaviour.