bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydrolysis of Queensland Fruit Fly, Bactrocera tryoni (Froggatt), Attractants: Kinetics and Implications for Biological Activity
Authors: Park Soo J. , Siderhurst Matthew S. , Jamie Ian , Taylor Phillip W.
Published: May 2016

Queensland fruit fly (Q-fly), Bactrocera tryoni (Froggatt), is a major insect pest of horticultural crops in Australia. Cuelure is the most commonly used attractant for monitoring as well as for management of Q-fly populations through the male annihilation technique and mass trapping. There has been some concern that cuelure is susceptible to hydrolysis, which would limit its usefulness under conditions of high humidity and give rise to inconsistent fly population monitoring. To give some insight into the hydrolysis of cuelure and two closely related compounds, melolure and a newly developed lure, 4-(4-(2,2,2-trifluoroacetoxy)phenyl)-2-butanone (RKTA), the kinetics of hydrolysis of these compounds were quantitatively investigated by gas chromatography–flame ionization detection. From the experimental data, we found the hydrolysis half-lives of cuelure, melolure, and RKTA, at a water concentration of 25 mol L–1, to be ~20 days, 22 h, and 1.2 min respectively. When extrapolated to a water concentration of 1.3 mmol L–1, corresponding to atmospheric conditions of 100 % relative humidity at 25°C, the half-lives are ~1660, ~51, and ~1.4 years respectively.
‘Laudably Communicating to the World’: Science in Sydney’s Public Culture, 1788–1821
Authors: Orthia Lindy A.
Published: May 2016

It has long been held that the general population of the British colony of New South Wales before the 1820s was neither exposed to nor interested in science, and that there was little home-grown science in Sydney at this time. This prevailing view, however, is based on a definition of science as institutionalized knowledge producer. In this paper I examine the Sydney colony between 1788 and 1821 through the lens of recent historiographical developments that have redefined science as a form of communicative action, and that incorporate the study of popular discourse centrally within histories of science. Under this approach, an examination of Sydney's mass media and popular culture reveals a widespread, rich and invested fascination with science among the colony's general population, and active contributions to public science discourse by ordinary colony residents.
F. Mueller, ‘The Murray-scrub, Sketched Botanically’, 1850: A Humboldtian Description of Mallee Vegetation
Authors: Maroske Sara , Darragh Thomas A.
Published: May 2016

Although best known as a descriptive botanist, Ferdinand Mueller published an early account of the South Australian Mallee in the style of his scientific hero, Alexander von Humboldt. This vegetation type is found across southern arid Australia and includes several distinctive botanical features that Mueller sought to highlight. While his article was republished twice, each issue was in German and consequently this work has tended to be overlooked in scholarship on the history of Australian botany. Mueller's article is introduced here along with a translation into English for the first time.
Eight river principles for navigating the science–policy interface
Authors: Parsons Melissa , Thoms Martin C. , Flotemersch Joseph E.
Published: May 2016

Scientists and policymakers often work together to develop policy about the sustainable use of river ecosystems. River science plays an important role in developing river policy but how can key aspects of river science be conveyed as a heuristic to navigate the interface between river science and river policy? This paper introduces eight principles that encapsulate the key properties of rivers to consider during the development of river policy: (1) rivers are social–ecological systems; (2) river ecosystems provide valuable ecosystem services; (3) tools should support policy development; (4) knowledge of river ecosystems will always be incomplete; (5) social–ecological systems require interdisciplinary perspectives; (6) science is one of many inputs to be considered; (7) heterogeneity and variability are characteristic of river ecosystems; and (8) scale awareness is essential in river ecosystems. Whereas policy challenges are associated with each principle, consideration of principles in the context of the issue at hand may increase the robustness of river policy and enhance the sustainability of river ecosystems. The eight principles are evaluated in relation to the Water Act 2007 and the draft Murray–Darling Basin Plan to demonstrate how the principles can enhance policy development in the area of water allocation.
The distribution, significance and vulnerability of Australian rhodolith beds: a review
Authors: Harvey A. S. , Harvey R. M. , Merton E.
Published: May 2016

Rhodolith beds are major marine benthic macrophyte communities, comparable in size and significance to kelp beds, seagrass meadows and coralline reefs. Rhodolith beds are currently ‘at risk’, both around the world and in Australia, from anthropogenic disturbances such as ocean acidification, coastal degradation and sedimentation. However, knowledge of rhodolith distribution in Australia is limited and beds are currently largely considered to be uncommon and isolated. An extensive review was undertaken using herbarium collections, and relevant scientific and grey literature (journal publications, marine surveys, ships’ logs, reference books and websites) for references to rhodoliths and rhodolith beds. Our study has shown that rhodoliths are common throughout 70% of Australia’s coastline, ranging from tropical to cold-temperate waters, down to 117m, forming a vast natural resource in terms of area covered, biodiversity and carbonate production. This review has created a solid foundation for future rhodolith research in Australia by documenting the extent of known rhodolith distribution. It will help inform and influence future research and policy planning on these largely unexplored, highly diverse marine ecosystems.
A mixed-methods impact evaluation of the feasibility of an initiative in small rural stores to improve access to fruit and vegetables
Authors: Palermo Claire , Gardiner Breeana , Gee Carena , Charaktis Stella , Blake Miranda
Published: May 2016

Retail stores are a promising setting for improving access to nutritious food. This study opportunistically evaluated an initiative that supported stores in small rural Victorian towns to sell fresh fruit and vegetables. It aimed to measure whether the initiative showed a trend towards improved access to fruit and vegetables in these rural communities. A mixed-methods, pre-post evaluation was employed to measure the range and price of 39 fruits and 45 vegetables, together with 15 interviews with stakeholders 2.5 years after the commencement of the initiative. Twenty-one of 35 eligible stores took up the initiative. Analysis of qualitative and quantitative data showed that the initiative improved the availability of and access to fruit and vegetables, and that stores have a role in improving access to fruit and vegetables. The overall range of fruit and vegetables increased over 18 months from a median of 10 varieties (n=10) to 17 varieties (n=15) (P=0.028) and the prices decreased over 12 months in five out of seven stores where data was available. The capacity to influence availability of fruit and vegetables was affected by time, human resources and community support. Sustaining change to fruit and vegetables access is challenging. Using stores for health promotion may be an effective strategy for improving rural populations’ fruit and vegetable intake.
Connecting Care in the Community: what works and what doesn't
Authors: Lucas Elizabeth , Halcomb Elizabeth , McCarthy Sandra
Published: May 2016

As the burden of chronic and complex disease grows, there is an emphasis on programs that enhance the quality of care within primary care. The Connecting Care in the Community (CCC) program is an example of the implementation of care integration. This qualitative study sought to explore the experiences general practice staff face in managing clients with chronic and complex care issues, and their perceptions of the contribution of the CCC program to this care. Seventeen general practice staff from 11 practices throughout the Illawarra/Shoalhaven region participated in semistructured interviews. Five main themes emerged: (1) awareness of the CCC program; (2) varying program exposure and value placed on the program; (3) practice ‘busyness’ and role confusion; (4) communication and information sharing; and (5) the need for staff education and knowledge of local resources. If policymakers and healthcare organisations can gain a better understanding of the experiences of general practice staff, they will be able to design and implement programs that best meet the needs of the providers that they are attempting to integrate.
Delphinidin prevents disuse muscle atrophy and reduces stress-related gene expression
Authors: Murata Motoki , Kosaka Reia , Kurihara Kana , Yamashita Shuya , Tachibana Hirofumi
Published: May 2016

Delphinidin is a member of the anthocyanidin class of plant pigments. We examined the effects of delphinidin on muscle atrophy. Oral administration of delphinidin suppressed the muscle weight loss induced by mechanical unloading. Microarray analysis showed that delphinidin suppresses the upregulation of oxidative stress-related gene expression, including the expression of Cbl-b. Thus, delphinidin may prevent unloading-mediated muscle atrophy.
Tumor-suppressive activity of 1,25-dihydroxyvitamin D3 against kidney cancer cells via up-regulation of FOXO3
Authors: Lee Jongsung , Park See-Hyoung
Published: May 2016

1,25-Dihydroxyvitamin D3 has been known to have the tumor-suppressive activity in various kinds of tumors. However, the exact effect and working mechanism of 1,25-dihydroxyvitamin D3 on the tumor-suppressive activity in human kidney cancer cells remains poorly understood. 1,25-Dihydroxyvitamin D3 has cytotoxicity to ACHN cells and inhibited ACHN cell proliferation compared to the vehicle control. 1,25-Dihydroxyvitamin D3 increased the expression of the cleaved PARP1, active Caspase3, Bax, and Bim but decreased the expression of Bcl2 in ACHN cells. Moreover, 1,25-dihydroxyvitamin D3 down-regulated the phosphorylated Akt and Erk which might lead to apoptosis through activation of FOXO3 in ACHN cells. Transfection of siRNA against FOXO3 attenuated the pro-apoptotic BimEL expression in ACHN cells treated with 1,25-dihydroxyvitamin D3. These results suggest that FOXO3 is involved in the apoptosis induced by 1,25-dihydroxyvitamin D3.
Mesp1 controls the speed, polarity, and directionality of cardiovascular progenitor migration
Authors: Chiapparo Giuseppe , Lin Xionghui , Lescroart Fabienne , Chabab Samira , Paulissen Catherine , Pitisci Lorenzo , Bondue Antoine , Blanpain Cédric
Published: May 2016

During embryonic development, Mesp1 marks the earliest cardiovascular progenitors (CPs) and promotes their specification, epithelial–mesenchymal transition (EMT), and cardiovascular differentiation. However, Mesp1 deletion in mice does not impair initial CP specification and early cardiac differentiation but induces cardiac malformations thought to arise from a defect of CP migration. Using inducible gain-of-function experiments during embryonic stem cell differentiation, we found that Mesp2, its closest homolog, was as efficient as Mesp1 at promoting CP specification, EMT, and cardiovascular differentiation. However, only Mesp1 stimulated polarity and directional cell migration through a cell-autonomous mechanism. Transcriptional analysis and chromatin immunoprecipitation experiments revealed that Mesp1 and Mesp2 activate common target genes that promote CP specification and differentiation. We identified two direct Mesp1 target genes, Prickle1 and RasGRP3, that are strongly induced by Mesp1 and not by Mesp2 and that control the polarity and the speed of cell migration. Altogether, our results identify the molecular interface controlled by Mesp1 that links CP specification and cell migration.
DSCR1 is required for both axonal growth cone extension and steering
Authors: Wang Wei , Rai Asit , Hur Eun-Mi , Smilansky Zeev , Chang Karen T. , Min Kyung-Tai
Published: May 2016

Local information processing in the growth cone is essential for correct wiring of the nervous system. As an axon navigates through the developing nervous system, the growth cone responds to extrinsic guidance cues by coordinating axon outgrowth with growth cone steering. It has become increasingly clear that axon extension requires proper actin polymerization dynamics, whereas growth cone steering involves local protein synthesis. However, molecular components integrating these two processes have not been identified. Here, we show that Down syndrome critical region 1 protein (DSCR1) controls axon outgrowth by modulating growth cone actin dynamics through regulation of cofilin activity (phospho/dephospho-cofilin). Additionally, DSCR1 mediates brain-derived neurotrophic factor–induced local protein synthesis and growth cone turning. Our study identifies DSCR1 as a key protein that couples axon growth and pathfinding by dually regulating actin dynamics and local protein synthesis.
Diaphanous formin mDia2 regulates CENP-A levels at centromeres
Authors: Liu Chenshu , Mao Yinghui
Published: May 2016

Centromeres of higher eukaryotes are epigenetically defined by centromere protein A (CENP-A), a centromere-specific histone H3 variant. The incorporation of new CENP-A into centromeres to maintain the epigenetic marker after genome replication in S phase occurs in G1 phase; however, how new CENP-A is loaded and stabilized remains poorly understood. Here, we identify the formin mDia2 as essential for stable replenishment of new CENP-A at centromeres. Quantitative imaging, pulse-chase analysis, and high-resolution ratiometric live-cell studies demonstrate that mDia2 and its nuclear localization are required to maintain CENP-A levels at centromeres. Depletion of mDia2 results in a prolonged centromere association of holiday junction recognition protein (HJURP), the chaperone required for CENP-A loading. A constitutively active form of mDia2 rescues the defect in new CENP-A loading caused by depletion of male germ cell Rac GTPase-activating protein (MgcRacGAP), a component of the small GTPase pathway essential for CENP-A maintenance. Thus, the formin mDia2 functions downstream of the MgcRacGAP-dependent pathway in regulating assembly of new CENP-A containing nucleosomes at centromeres.
How Mesp1 makes a move
Authors: Kelly Robert G.
Published: May 2016

The transcription factors Mesp1 and Mesp2 have essential roles in the migration and specification of multipotent progenitor cells at the onset of cardiogenesis. Chiapparo et al. (2016. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201505082) identify common Mesp functions in fate specification and Mesp1-specific targets controlling the speed and direction of progenitor cell migration.
The transcription factor Zeb2 regulates development of conventional and plasmacytoid DCs by repressing Id2
Authors: Scott Charlotte L. , Soen Bieke , Martens Liesbet , Skrypek Nicolas , Saelens Wouter , Taminau Joachim , Blancke Gillian , Van Isterdael Gert , Huylebroeck Danny , Haigh Jody , Saeys Yvan , Guilliams Martin , Lambrecht Bart N. , Berx Geert
Published: May 2016

Plasmacytoid dendritic cells (DCs [pDCs]) develop from pre-pDCs, whereas two lineages of conventional DCs (cDCs; cDC1s and cDC2s) develop from lineage-committed pre-cDCs. Several transcription factors (TFs) have been implicated in regulating the development of pDCs (E2-2 and Id2) and cDC1s (Irf8, Id2, and Batf3); however, those required for the early commitment of pre-cDCs toward the cDC2 lineage are unknown. Here, we identify the TF zinc finger E box–binding homeobox 2 (Zeb2) to play a crucial role in regulating DC development. Zeb2 was expressed from the pre-pDC and pre-cDC stage onward and highly expressed in mature pDCs and cDC2s. Mice conditionally lacking Zeb2 in CD11c+ cells had a cell-intrinsic reduction in pDCs and cDC2s, coupled with an increase in cDC1s. Conversely, mice in which CD11c+ cells overexpressed Zeb2 displayed a reduction in cDC1s. This was accompanied by altered expression of Id2, which was up-regulated in cDC2s and pDCs from conditional knockout mice. Zeb2 chromatin immunoprecipitation analysis revealed Id2 to be a direct target of Zeb2. Thus, we conclude that Zeb2 regulates commitment to both the cDC2 and pDC lineages through repression of Id2.
“That British Sound”: Talk of Nationalness in Global Capitalism
Authors: Ng Kwai Hang , Skotnicki Tad
Published: May 2016

This article proposes the concept of “nationalness” to account for the persistent circulation of national labels as a tool of distinction. We argue that the concept expands on the traditional conception of nation as country of origin to include cultural notions of sensuous and aesthetic qualities that are semiotically tied to national “essence.” Nationalness connects products to nations not only through indexicality but also iconicity. It is made possible through “rhematization,” the process of appropriating signs whose interpretants are taken to be iconic. Talk of nationalness enables consumers to make sense of national labels in today’s world where the both ends of capitalism—manufacturing and consumption—are thoroughly global. Instead of confining our analytical focus to top-down national branding projects, we examine how consumers invoke the idea of a nation to describe their uses of industrial products. Our case study is the popular concept of “British sound” circulated among audiophiles. Drawing on online discussions of an internet community, the article shows that audiophiles refer to Britishness as a key mechanism to distinguish their consumption experiences from mass market consumption. We further show how the label “British sound” can be used to describe the experiences of consumer products that are no longer manufactured in Britain.
On Not Being a Sign: Gold’s Semiotic Claims
Authors: Ferry Elizabeth
Published: May 2016

In this essay I examine gold’s semiotic claims in the context of perceptions of gold in global financial markets, beginning with an intriguing parallel drawn by my informants between gold and Bitcoin. I make several interrelated arguments: (1) that in a variety of cultural contexts, gold has become a site for a very particular semiotic claim—that of not signifying at all; (2) that in the contemporary instance of physical gold in relation to other assets based on gold, this is expressed in an attention to gold’s material, tactile qualities (represented both iconically and indexically), and its extrasocial character; (3) that similar claims are made for Bitcoin, often drawing on a “digital metallism,” and idiom of gold; and (4) that the self-reflexive semiotic properties of gold have a long and diverse cultural history. In concluding, I speculate on how gold’s particular semiotic stance reflects one possible articulation of nature and convention as sources of meaning.
Discourses, Legitimization, and the Construction of Acadianité
Authors: LeBlanc Mélanie , Boudreau Annette
Published: May 2016

This article examines how a discourse of nationhood has developed and evolved in Acadie since the nineteenth century. It describes the important role played by language ideologies in the construction of competing discourses in the Francophone world. The discursive changes that have occurred since the 1960s in relation to the distribution of material and symbolic resources will be explored, with a specific focus on the resemioticization of French vernaculars in artistic creation and tourism. Finally, the authors will illustrate how the Acadians have transformed a discourse of stigmatization into a discourse of differentiation by rejecting the standard language ideology that has traditionally defined the Franco-Canadian community. The examples of artistic production and tourism will be used to illustrate how Acadians have profited from the adoption of this discourse in national and international markets.
The Late-Night Infomercial as an Electronic Site of Ritual Self-Transformation: The Case of “Personal Power”
Authors: Glick Douglas J.
Published: May 2016

This article argues that a late-night television infomercial displays the structure of a ritual rite of passage. It tracks how the infomercial creates a state of ideological liminality that is redressed by a series of poetic (linguistic) parallelisms. This culminates in a trope of incorporation that transforms the social identity of the viewer from “unsuccessful” to “successful.” Ritual in form yet electronic in medium, the conclusion relates the ritual merging of consumer and new product type in processes of commodification to the ways in which cultural analogy, ideological and otherwise, moves certain kinds of social meanings into new arenas of practice.
The Offline and Online Mediatization of Psychoanalysis in Buenos Aires
Authors: Marsilli-Vargas Xochitl
Published: May 2016

This article explores the mediatized nature of the circulation of psychoanalysis in Buenos Aires, Argentina, by focusing on psychoanalytic talk and listening practices, inside and outside the clinical setting. It shows that mediatization processes, which link institutional practices to processes of communication and commoditization (Agha 2011a), are key for understanding how psychoanalytic knowledge (including its lexical register) and its therapeutic practice get reproduced in fractionally congruent forms within everyday interactions across Buenos Aires. Specific emphasis is placed on the many uptake formulations of psychoanalytic practice that are observed today, on the scale-changing effects of mediatization, and on changes in the propinquity of interactants in psychoanalytic encounters.
Resistance in Strawberry Isolates of Colletotrichum acutatum from Florida to Quinone-Outside Inhibitor Fungicides
Authors: Forcelini Bruna B. , Seijo Teresa E. , Amiri Achour , Peres Natalia A.
Published: May 2016

Anthracnose fruit rot of strawberry, caused by Colletotrichum acutatum, is a major disease in Florida and frequent quinone-outside inhibitor (QoI) fungicide applications are needed for disease control. From 1994 to 2014, 181 C. acutatum isolates were collected from multiple strawberry fields in Florida with or without QoI spray history. Sensitivity to azoxystrobin and pyraclostrobin was tested based upon mycelial growth and germ tube elongation inhibition. Mean effective concentration where growth was reduced by 50% (EC50) values for isolates collected prior to 2013 based upon mycelial growth were 0.22 and 0.013 μg/ml and upon germ tube elongation were 0.57 and 0.03 μg/ml for azoxystrobin and pyraclostrobin, respectively. Mycelial growth and germ tube elongation of 48 isolates collected in 2013 and 2014 were not inhibited with azoxystrobin at 3 μg/ml and pyraclostrobin at 0.110 μg/ml. A fungicide discriminatory dose assay indicated that 43 of the 48 isolates had EC50 values higher than 100 and 10 μg/ml for azoxystrobin and pyraclostrobin, respectively. Azoxystrobin and pyraclostrobin sprayed preventively on strawberry fruit inoculated with C. acutatum failed to control resistant isolates. Sequencing of the cytochrome b gene of sensitive and resistant isolates showed that QoI-resistant isolates contained either G143A or F129L amino acid substitutions.
The Effect of Different Temperatures and Moisture Levels on Survival of Calonectria pseudonaviculata in Boxwood Leaves and Twigs and as Microsclerotia Produced in Culture
Authors: Shishkoff Nina , Camp Mary J.
Published: May 2016

Leaves and twig sections of boxwood infected with Calonectria pseudonaviculata were incubated in sand at two moisture levels (36% [carrying capacity] and 5% water [vol/vol]) and at five temperatures (–10, 0, 10, 20, and 30°C). Percent sporulation from monthly tissue samples plated on glucose yeast-extract tyrosine media declined to zero after 5 months at 30°C and after 7 months at –10°C. At 0, 10, and 20°C, sporulation was observed through 30 months. Statistical analysis of data collected over 16 months of sampling showed a significant effect for temperature in all sample types, with maximum survival at 10°C. For discrete microsclerotia grown on cellophane sheets, sporulation was not observed after 2 months at 30°C or after7 months at –10°C. At all other temperatures, they continued to germinate over the 30 months of sampling. Statistical analysis showed significant effects for temperature and moisture level, with maximum survival at 0°C in moist soil over 16 months. These results suggest that extremes of heat and cold will kill the pathogen in plant debris but, at moderate temperatures, it will remain in soil for long periods, making replanting in affected sites in well-moderated climates difficult.
Zika virus infection as an unexpected finding in a Leptospirosis patient
Authors: Biron Antoine , Cazorla Cécile , Amar Julien , Pfannstiel Anne , Dupont-Rouzeyrol Myrielle , Goarant Cyrille
Published: May 2016

Introduction:

Areas where leptospirosis and arboviruses are endemic largely overlap in the tropics. However, the number of arbovirus infections is usually much higher. The initial clinical presentation can be highly confusing; therefore, laboratory confirmation is key to an accurate diagnosis.

Case Presentation:

A 19–year–old man presented to a peripheral health centre with an acute febrile illness. Dengue was initially suspected, but the patient deteriorated to a shock syndrome. Leptospirosis as well as a co-infection with Zika virus were both confirmed in the laboratory, the latter being clinically masked in this dual infection.

Conclusion:

This case highlights the importance of not only considering the differential diagnosis of acute febrile syndromes, but also to consider the possibility of dual infections in the context of global spread of arboviruses. The specific context of travellers returning from endemic areas and pregnant women is also highlighted and discussed.
Utilizing Social Media and Video Games to Control #DIY Microscopes
Authors: Leblanc-Latour Maxime , Bryan Craig , Pelling Andrew
Published: May 2016

Open-source lab equipment is becoming more widespread with the popularization of fabrication tools such as 3d-printers, laser cutters, CNC machines, open source microcontrollers and open source software. Although many pieces of common laboratory equipment have been developed, software control of these items is sometimes lacking. Specifically, control software that can be easily implemented and enable user-input and control over multiple platforms (PC, smartphone, web, etc.). The aim of this proof-of-principle study was to develop and implement software for the control of a low-cost, 3d-printed microscope. Here, we present two approaches, which enable microscope control by exploiting the functionality of the social media platform Twitter or player actions inside of the videogame Minecraft. The microscope was constructed from a modified web-camera and implemented on a Raspberry Pi computer. Four aspects of microscope control were tested, including single image capture, focus control and time-lapse imaging. The Twitter-embodiment enabled users to send “tweets” directly to the microscope. Image data acquired by the microscope was then returned to the user through a Twitter reply and stored permanently on the photo-sharing platform Flickr, along with any relevant metadata. Local control of the microscope was also implemented by utilizing the video game Minecraft, in situations where Internet connectivity is not present or stable. A virtual laboratory was constructed inside the Minecraft world and player actions inside the laboratory were linked to specific microscope functions. Here, we present the methodology and results of these experiments and discuss possible limitations and future extensions of this work.
Figure-ground organization in visual cortex for natural scenes
Authors: Williford Jonathan R. , von der Heydt Rüdiger
Published: May 2016

Figure-ground organization and border-ownership assignment are essential for understanding natural scenes. It has been shown that many neurons in the macaque visual cortex signal border-ownership in displays of simple geometric shapes such as squares, but how well these neurons resolve border-ownership in natural scenes is not known. We studied area V2 neurons in behaving macaques with static images of complex natural scenes. We found that about half of the neurons were border-ownership selective for contours in natural scenes and this selectivity originated from the image context. The border-ownership signals emerged within 70 ms after stimulus onset, only~30 ms after response onset. A substantial fraction of neurons were highly consistent across scenes. Thus, the cortical mechanisms of figure-ground organization are fast and efficient even in images of complex natural scenes. Understanding how the brain performs this task so fast remains a challenge.

Significance Statement

Here we show, for the first time, that neurons in primate visual area V2 signal border-ownership for objects in complex natural scenes. Surprisingly, these signals appear as early as the border-ownership signals for simple figure displays. In fact, they emerge well before object selective activity appears in infero-temporal cortex, which rules out feedback from that region as an explanation. Thus, “objectness” is detected by extremely fast mechanisms that do not depend on feedback from the known object-recognition centers.
Stable host gene expression in the gut of adultDrosophila melanogasterwith different bacterial mono-associations
Authors: Elya Carolyn , Zhang Vivian , Ludington Will , Eisen Michael B
Published: May 2016

There is growing evidence that the microbes found in the digestive tracts of animals influence host biology, but we still do not understand how this comes about. Here, we evaluated how different microbial species commonly associated with laboratory-rearedDrosophila melanogasterimpact host biology at the level of gene expression in the dissected adult gut or the entire adult organism. We observed that guts from gnotobiotic animals associated from the embryonic stage with either zero, one or three bacterial species demonstrated indistinguishable transcriptional profiles. Additionally, we found that the gut transcriptional profiles of animals reared in the presence of the yeastSaccharomyces cerevisiaealone or in combination with bacteria could recapitulate those of conventionally-reared animals. In contrast, we found whole body transcriptional profiles of conventionally-reared animals were distinct from all of the gnotobiotic treatments tested. Our data suggest that adult flies are insensitive to the ingestion of different bacterial species but that prior to adulthood, different microbes impact the host in ways that lead to global transcriptional differences observable across the whole adult body.
Advances in the integration of transcriptional regulatory information into genome-scale metabolic models
Authors: Vivek-Ananth R.P. , Samal Areejit
Published: May 2016

A major goal of systems biology is to build predictive computational models of cellular metabolism. Availability of complete genome sequences and wealth of legacy biochemical information has led to the reconstruction of genome-scale metabolic networks in the last 15 years for several organisms across the three domains of life. Due to paucity of information on kinetic parameters associated with metabolic reactions, the constraint-based modelling approach, flux balance analysis (FBA), has proved to be a vital alternative to investigate the capabilities of reconstructed metabolic networks. In parallel, advent of high-throughput technologies has led to the generation of massive amounts ofomicsdata on transcriptional regulation comprising mRNA transcript levels and genome-wide binding profile of transcriptional regulators. A frontier area in metabolic systems biology has been the development of methods to integrate the available transcriptional regulatory information into constraint-based models of reconstructed metabolic networks in order to increase the predictive capabilities of computational models and understand the regulation of cellular metabolism. Here, we review the existing methods to integrate transcriptional regulatory information into constraint-based models of metabolic networks.
Rapid automated superposition of shapes and macromolecular models using spherical harmonics
Authors: Konarev Petr V. , Petoukhov Maxim V. , Svergun Dmitri I.
Published: May 2016

A rapid algorithm to superimpose macromolecular models in Fourier space is proposed and implemented (SUPALM). The method uses a normalized integrated cross-term of the scattering amplitudes as a proximity measure between two three-dimensional objects. The reciprocal-space algorithm allows for direct matching of heterogeneous objects including high- and low-resolution models represented by atomic coordinates, beads or dummy residue chains as well as electron microscopy density maps and inhomogeneous multi-phase models (e.g.of protein–nucleic acid complexes). Using spherical harmonics for the computation of the amplitudes, the method is up to an order of magnitude faster than the real-space algorithm implemented inSUPCOMBby Kozin & Svergun [J. Appl. Cryst.(2001),34, 33–41]. The utility of the new method is demonstrated in a number of test cases and compared with the results ofSUPCOMB. The spherical harmonics algorithm is best suited for low-resolution shape models,e.g. those provided by solution scattering experiments, but also facilitates a rapid cross-validation against structural models obtained by other methods.
The vacuum ultraviolet beamline/endstations at NSRL dedicated to combustion research
Authors: Zhou Zhongyue , Du Xuewei , Yang Jiuzhong , Wang Yizun , Li Chaoyang , Wei Shen , Du Liangliang , Li Yuyang , Qi Fei , Wang Qiuping
Published: May 2016

An undulator-based vacuum ultraviolet (VUV) beamline (BL03U), intended for combustion chemistry studies, has been constructed at the National Synchrotron Radiation Laboratory (NSRL) in Hefei, China. The beamline is connected to the newly upgraded Hefei Light Source (HLS II), and could deliver photons in the 5–21 eV range, with a photon flux of 1013 photons s−1at 10 eV when the beam current is 300 mA. The monochromator of the beamline is equipped with two gratings (200 lines mm−1and 400 lines mm−1) and its resolving power is 3900 at 7.3 eV for the 200 lines mm−1grating and 4200 at 14.6 eV for the 400 lines mm−1grating. The beamline serves three endstations which are designed for respective studies of premixed flame, fuel pyrolysis in flow reactor, and oxidation in jet-stirred reactor. Each endstation contains a reactor chamber, an ionization chamber where the molecular beam intersects with the VUV light, and a home-made reflectron time-of-flight mass spectrometer. The performance of the beamline and endstations with some preliminary results is presented here. The ability to detect reactive intermediates (e.g.H, O, OH and hydroperoxides) is advantageous in combustion chemistry research.
Characterization of an X-ray mirror mechanical bender for the European XFEL
Authors: Vannoni Maurizio , Freijo Martín Idoia , Sinn Harald
Published: May 2016

One of the classical devices used to tune a mirror on an X-ray optical setup is a mechanical bender. This is often designed in such a way that the mirror is held with clamps on both ends; a motor is then used to put a torque on the clamps, inducing a cylindrical shape of the mirror surface. A mechanical bender with this design was recently characterized, to bend a 950 mm-long mirror up to a radius of curvature of 10 km. The characterization was performed using a large-aperture Fizeau interferometer with an angled incidence setup. Some particular and critical effects were investigated, such as calibration, hysteresis, twisting and long-term stability.
Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging
Authors: Newman Justin A. , Zhang Shijie , Sullivan Shane Z. , Dow Ximeng Y. , Becker Michael , Sheedlo Michael J. , Stepanov Sergey , Carlsen Mark S. , Everly R. Michael , Das Chittaranjan , Fischetti Robert F. , Simpson Garth J.
Published: May 2016

Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation, two-photon-excited fluorescence and bright field by laser transmittance were all acquired simultaneously with perfect image registry at up to video-rate (15 frames s−1). A simple change in the incident wavelength enabled simultaneous imaging by two-photon-excited ultraviolet fluorescence, one-photon-excited visible fluorescence and laser transmittance. Development of an analytical model for the signal-to-noise enhancement afforded by synchronous digitization suggests a 15.6-fold improvement over previous photon-counting techniques. This improvement in turn allowed acquisition on nearly an order of magnitude more pixels than the preceding generation of instrumentation and reductions of well over an order of magnitude in image acquisition times. These improvements have allowed detection of protein crystals on the order of 1 µm in thickness under cryogenic conditions in the beamline. These capabilities are well suited to support serial crystallography of crystals approaching 1 µm or less in dimension.
Generation of ultra-large-bandwidth X-ray free-electron-laser pulses with a transverse-gradient undulator
Authors: Prat Eduard , Calvi Marco , Reiche Sven
Published: May 2016

A new and simple method to generate X-ray free-electron-laser radiation with unprecedented spectral bandwidth above the 10% level is presented. The broad bandwidth is achieved by sending a transversely tilted beam through a transverse-gradient undulator. The extent of the bandwidth can easily be controlled by variation of the beam tilt or the undulator gradient. Numerical simulations confirm the validity and feasibility of this method.
Using reasoned imagination to learn about cascading hazards: a pilot study
Authors: Mignan Arnaud , Scolobig Anna , Sauron Anne
Published: May 2016

Purpose

– The purpose of this paper is to present the results of a pilot study involving high school teachers in natural sciences. The aim was to foster critical thinking about cascading hazards via the use of reasoned imagination. Cascading phenomena can lead to extreme catastrophes and are thus a challenge for disaster prevention and management.

Design/methodology/approach

– Following a presentation listing some known cascading phenomena, the participants completed a questionnaire consisting of a blank hazard correlation matrix (HCM) and some open-ended questions. The HCM qualitatively described possible interactions between 16 different perils selected from a large spectrum of natural, technological and socio-economic hazards.

Findings

– Most participants were able to describe cascading phenomena within the HCM by reducing them into sets of one-to-one interactions. Based on their experience and imagination, the participants foresaw additional interactions that were not discussed, never observed but are scientifically plausible. The majority of the respondents reported that they learnt something new and wanted to learn more about cascading hazards.

Originality/value

– The HCM is especially effective in translating complex hazard scenarios into basic interactions and vice versa. Being imaginative (here via the use of reasoned imagination) and accessible, the HCM could be used as basis for transformative learning in the education of the public and of practitioners on the role of cascading hazards in catastrophes.
Competence-based system development for post-disaster project management
Authors: von Meding Jason , Wong Joel , Kanjanabootra Sittimont , Taheri Tafti Mojgan
Published: May 2016

Purpose

– One of the key elements contributing to successful post-disaster project teams is individual competence. Each project participant brings his or her own knowledge, experience and ideas to the collective. The kind of chaotic and fragmented environment that is common in post-disaster scenarios presents specific barriers to the success of projects, which can be mitigated by ensuring that staff members possess competencies appropriate for their deployment to particular contexts. The paper aims to discuss these issues.

Design/methodology/approach

– The study utilizes a mixed-methods approach, incorporating unstructured interviews to extract key factors of competence, project barriers and strategy, and a subsequent questionnaire survey, designed to quantify the various elements. Interviews were undertaken and analysed using a cognitive mapping procedure, while survey data were processed using SPSS. The data were then utilized in the development of a software prototype using Design Science Research methodology, capable of modelling the deployment of staff under various disaster scenarios.

Findings

– Analysis of the survey and cognitive mapping data, in conjunction with relevant established frameworks, has allowed the classification of relevant competency elements. These elements have subsequently been measured and modelled into the competency-based tool and developed into a working prototype.

Originality/value

– The developed system offers novel disaster competency assessment criteria. The system contains a variety of real-life scenarios derived from extensive data collection. These multi-hazard scenarios are embedded with knowledge and competency valuation criteria that can facilitate actors to assess their team’s knowledge based on selective scenarios. In disaster response, time is a critical element, and this tool assists decision makers. It can enable disaster response actors to evaluate and assemble the appropriate personnel to deploy into disaster areas and into specific types of disaster environment.
A communications intervention to motivate disaster risk reduction
Authors: Sanquini Anne M. , Thapaliya Sundar M. , Wood Michele M.
Published: May 2016

Purpose

– The purpose of this paper is to apply social theory to the creation of a mass-media communications intervention designed to encourage earthquake-resistant construction in Nepal.

Design/methodology/approach

– A three-step process was employed in this study: first, a narrative literature review was completed regarding the motivation of protective action. Second, key informant elicitation interviews with 15 community members at five public schools who supported making their buildings earthquake-resistant informed the script for a documentary film. Finally, the film was reviewed with stakeholders, plus 16 community members associated with a school in need of seismic work. Sociograms were used to determine relative closeness of the study participants to the film role models.

Findings

– Motivating factors identified in the literature synthesis were included in the film, which focussed on effective actions taken by role models, and avoided the use of fear-based appeals. Key informant interviews yielded role-modeling details for the film script, including triggers and obstacles faced by the community members, and outcomes of their actions. Sociogram outcomes guided film editing and increased relative screen time for those community members with whom the study participants felt greater closeness. A pretest-posttest cluster randomized trial (details reported elsewhere) showed greater gains in knowledge, perceived outcome effectiveness, and intended behaviors among intervention film viewers than control participants.

Originality/value

– This three-step process yielded the information required by a practitioner to develop a theory-based, culturally appropriate mass-media intervention designed to motivate reduction of disaster risk.
The role of moral disengagement in supply chain management research
Authors: Eriksson David
Published: May 2016

Purpose

The study aims to explain the role of moral disengagement in supply chain management (SCM) research and the challenges that arise if the theory is used beyond its inherent limitations.

Design/methodology/approach

Conceptual paper based on how Bandura developed and used moral disengagement.

Findings

Moral disengagement can be used validly in SCM research. The theory should not to be applied to the supply chain itself, but SCM can be seen as an environment that is part of a reciprocal exchange, which shapes human behavior.

Research limitations/implications

The paper suggests a new theory for a better understanding of business ethics, corporate social responsibility and sustainability in SCM. Furthermore, the paper outlines how the theory should be used and some challenges that remain.

Originality/value

SCM researchers have shown how to apply a theory from psychology to SCM, which could progress to several areas of the research field. The paper also highlights an inconsistency in the use of the theory and explains how it should be used in SCM research.
Socioeconomic status and primary health service use for mental health problems among immigrants with short and long lengths of stay
Authors: Straiton Melanie Lindsay , Reneflot Anne , Diaz Esperanza
Published: May 2016

Purpose

– High socioeconomic status (SES) is associated with better health and lower use of health care services in the general population. Among immigrants, the relationship appears less consistent. The purpose of this paper is to determine if the relationship between income level (a proxy for SES) and use of primary health care services for mental health problems differs for natives and five immigrant groups in Norway. It also explores the moderating effect of length of stay (LoS) among immigrants.

Design/methodology/approach

– Using data from two registers with national-level coverage, logistic regression analyses with interactions were carried out to determine the association between income level and having used primary health care services for mental health problems.

Findings

– For Norwegian men and women there was a clear negative relationship between income and service use. Interaction analyses suggested that the relationship differed for all immigrant groups compared with Norwegians. When stratifying by LoS, income was not associated with service use among recently arrived immigrants but was negatively associated among immigrants staying more than two years (with the exception of Pakistani and Iraqi women).

Research limitations/implications

– Country of origin and LoS should be considered when applying measures of SES in immigrant health research.

Social implications

– There may be an initial transition period for recently arrived immigrants where competing factors mask the association between SES and service use.

Originality/value

– This study benefits from nationwide coverage, eliminating self-selection biases. It demonstrates the complexity of the relationship between SES and health care use.