bluuebunny/crossref_metadata_2025 huggingface dataset:
Stress Relaxing Simulation on Digital Rock: Characterize Attenuation Due To Wave‐Induced Fluid Flow and Scattering
Authors: Zhu Wei , Zhao Luanxiao , Yang Zhifang , Cao Hong , Wang Yirong , Chen Wei , Shan Rui
Published: January 2023

Digital rock physics (DRP) is essential to understand the wave elastic and attenuation characteristics of complex porous rocks, offering insights into interpreting seismic and sonic logging data. Most of the existing DRP technology can only simulate wave‐induced fluid flow (WIFF) or scattering effects separately in a relatively narrow or single‐frequency band. To incorporate both the effects of WIFF and elastic scattering simultaneously, we develop a stress relaxing simulation (SRS) technique on digital rocks to characterize the P wave dispersion and attenuation signatures in a broad frequency band. We propose to use the combined methodology of curve resampling and wavelet‐packet denoising to suppress the high‐frequency noise of the stress relaxation curve. For a digital rock containing pore‐crack elements, we demonstrate that SRS is capable of characterizing the attenuation due to squirt flow and scattering as well as their interactions. The simulation results of wave dispersion and attenuation using SRS are overall consistent with other well‐developed DRP technology of transmitted wave simulation (high‐frequency band), forced oscillating simulation (intermediate‐frequency band), and static simulation (low‐frequency band), suggesting the reliability and robustness of the developed methodology. We also apply the SRS on a realistic 2D digital rock of porous sandstone. It is found that the attenuation due to elastic scattering of the microscopic heterogeneities is significantly higher than that caused by squirt flow, which might be caused by the lack of pore connectivity impeding the fluid exchange at the microscopic scale due to the limitation of 2D digital porous rocks.
E‐W and S‐N Differences in the Sedimentary Cover and Crystalline Crust in the Cratonic Ordos Basin From Receiver Function Analysis
Authors: Wei Zigen , Chu Risheng , Wang Jianqiang , Ling Yuan , Hu Jintao , Wu Shanshan
Published: January 2023

Thicknesses and Vp/Vs ratios of sedimentary cover and crystalline crust are key parameters to study basin properties and evolution. In this paper, we develop a hi‐ki method of receiver functions to estimate the thickness (h) and Vp/Vs ratio (k) for each layer based on the arrival times of Ps converted and PpPs multiple phases of the layer, and apply the method to invert for h and k values of the sedimentary and bedrock layers in the Ordos Basin. S‐N and E‐W differences in structure across approximately 109°E are observed in both the sedimentary cover and crystalline crust. The sedimentary thickness generally increases from east to west and reaches 7.9 km on the southwestern edge. Vp/Vs ratios are complex with an average >1.9 and overall are lower than 1.75 in the northwestern part. The bedrock thickness and Vp/Vs ratio vary from 31.2 to 45.0 km and from 1.60 to 1.82, with average values of 39.62 km and 1.74, respectively. The crystalline crust still retains the long‐term cratonic stability and characteristics. The observed S‐N and E‐W differences in the sedimentary and bedrock layers may be the combined results of inhomogeneity in original nucleation and later complex and long‐term tectonic evolution.
Diverse Lava Flow Morphologies in the Stratigraphy of the Jezero Crater Floor
Authors: Alwmark S. , Horgan B. , Udry A. , Bechtold A. , Fagents S. , Ravanis E. , Crumpler L. , Schmitz N. , Cloutis E. , Brown A. , Flannery D. , Gasnault O. , Grotzinger J. , Gupta S. , Kah L. , Kelemen P. , Kinch K. , Núñez J.
Published: January 2023

We present a combined geomorphologic, multispectral, and geochemical analysis of crater floor rocks in Jezero crater based on data obtained by the Mast Camera Zoom and SuperCam instruments onboard the NASA Mars 2020 Perseverance rover. The combined data from this analysis together with the results of a comparative study with geologic sites on Earth allows us to interpret the origins of rocks exposed along the Artuby ridge, a ∼900 m long scarp of lower Máaz formation rocks. The ridge exposes rocks belonging to two morphologically distinct members, Artuby and Rochette, both of which have basaltic composition and are spectrally indistinguishable in our analysis. Artuby rocks consist of morphologically distinct units that alternate over the ridge, bulbous, hummocky, layers with varying thicknesses that in places appear to have flowed over underlying strata, and sub‐planar thinner laterally continuous layers with variable friability. The Rochette member has a massive appearance with pronounced pitting and sub‐horizontal partings. Our findings are most consistent with a primary igneous emplacement as lava flows, through multiple eruptions, and we propose that the thin layers result either from preferential weathering, interbedded ash/tephra layers, ʻaʻā clinker layers, or aeolian deposition. Our analyses provide essential geologic context for the Máaz formation samples that will be returned to Earth and highlight the diversity and complexity of geologic processes on Mars not visible from orbit.
Photoexcitation dynamics and energy engineering in supramolecular doping of organic conjugated molecules
Authors: An Xiang , Wei Chuanxin , Bai Lubing , Zhou Jun , Wang Le , Han Yamin , Sun Lili , Lin Jinyi , Liu Heyuan , Li Jiewei , Xu Man , Ling Haifeng , Xie Linghai , Huang Wei
Published: January 2023

Doping and blending strategies are crucial means to precisely control the excited states and energy level in conjugated molecular systems. However, effective models and platforms are rarely proposed to systematically explore the effects of the formation of trapped doped centers on heterogeneous structures, energy level and ultrafast photophysical process. Herein, for deeply understanding the impact of molecular doping in film energy levels and photoexcitation dynamics, we set a supramolecular N-B coordination composed by the conjugated molecules of pyridine functionalized diarylfluorene (host material), named as ODPF-Phpy and ODPF-(Phpy)2, and the molecule of tris(perfluorophenyl)borane (BCF) (guest material). The generation of the molecular-level coordination bond increased the binding energy of N atoms and tuned the band-gap, leading to a new fluorescent emission center with longer excitation wavelength and emission wavelength. The intermolecular Förster resonance energy transfer (FRET) in blending films make it present inconsistent fluorescent behaviors compared to that in solution. The charge transfer (CT) state of N-B coordinated compounds and the changed dielectric constant of blending films resulted in a large PL spectra red-shift with the increased dopant ratio, causing a wide-tunable fluorescent color. The excited state behaviors of two compounds in blending system was further investigated by the transient absorption (TA) spectroscopy. Finally, we found supramolecular coordination blending can effectively improve the films’ photoluminescence quantum yield (PLQY) and conductivity. We believe this exploration in the internal coordination mechanisms would deepen the insights about doped semiconductors and is helpful in developing novel high-efficient fluorescent systems.
SGK2 promotes prostate cancer metastasis by inhibiting ferroptosis via upregulating GPX4
Authors: Cheng Lulin , He Qingliu , Liu Bing , Chen Liang , Lv Fang , Li Xuexiang , Li Yunxue , Liu Chunyu , Song Yarong , Xing Yifei
Published: January 2023

Recent research has shown that ferroptosis, the iron-dependent accumulation of lipid peroxides that leads to cell death, suppresses cancer metastasis. However, the role of ferroptosis in prostate cancer metastasis has not been completely elucidated. In the current study, we identified the essential role of serum/glucocorticoid regulated kinase 2 (SGK2) in promoting prostate cancer metastasis by inhibiting ferroptosis. We found that the expression of SGK2 was higher in metastatic prostate cancer and predicted poor clinical outcomes. SGK2 knockdown inhibited the metastatic capacity of prostate cancer cells in vivo and in vitro, while SGK2 overexpression inhibited ferroptosis and facilitated prostate cancer metastasis by phosphorylating the Thr-24 and Ser-319 sites of forkhead box O1 (FOXO1). This process induced the translocation of FOXO1 from the nucleus to the cytoplasm, relieving the inhibitory effect of FOXO1 on glutathione peroxidase 4 (GPX4). These findings delineated a novel role of SGK2 in ferroptosis regulation of prostate cancer metastasis, identifying a new key pathway driving prostate cancer metastasis and potentially providing new treatment strategies for metastatic prostate cancer.
Entorhinal grid-like codes and time-locked network dynamics track others navigating through space
Authors: Wagner Isabella C. , Graichen Luise P. , Todorova Boryana , Lüttig Andre , Omer David B. , Stangl Matthias , Lamm Claus
Published: January 2023

Navigating through crowded, dynamically changing environments requires the ability to keep track of other individuals. Grid cells in the entorhinal cortex are a central component of self-related navigation but whether they also track others’ movement is unclear. Here, we propose that entorhinal grid-like codes make an essential contribution to socio-spatial navigation. Sixty human participants underwent functional magnetic resonance imaging (fMRI) while observing and re-tracing different paths of a demonstrator that navigated a virtual reality environment. Results revealed that grid-like codes in the entorhinal cortex tracked the other individual navigating through space. The activity of grid-like codes was time-locked to increases in co-activation and entorhinal-cortical connectivity that included the striatum, the hippocampus, parahippocampal and right posterior parietal cortices. Surprisingly, the grid-related effects during observation were stronger the worse participants performed when subsequently re-tracing the demonstrator’s paths. Our findings suggests that network dynamics time-locked to entorhinal grid-cell-related activity might serve to distribute information about the location of others throughout the brain.
Single-cell analysis reveals prognostic fibroblast subpopulations linked to molecular and immunological subtypes of lung cancer
Authors: Hanley Christopher J. , Waise Sara , Ellis Matthew J. , Lopez Maria A. , Pun Wai Y. , Taylor Julian , Parker Rachel , Kimbley Lucy M. , Chee Serena J. , Shaw Emily C. , West Jonathan , Alzetani Aiman , Woo Edwin , Ottensmeier Christian H. , Rose-Zerilli Matthew J. J. , Thomas Gareth J.
Published: January 2023

Fibroblasts are poorly characterised cells that variably impact tumour progression. Here, we use single cell RNA-sequencing, multiplexed immunohistochemistry and digital cytometry (CIBERSORTx) to identify and characterise three major fibroblast subpopulations in human non-small cell lung cancer: adventitial, alveolar and myofibroblasts. Alveolar and adventitial fibroblasts (enriched in control tissue samples) localise to discrete spatial niches in histologically normal lung tissue and indicate improved overall survival rates when present in lung adenocarcinomas (LUAD). Trajectory inference identifies three phases of control tissue fibroblast activation, leading to myofibroblast enrichment in tumour samples: initial upregulation of inflammatory cytokines, followed by stress-response signalling and ultimately increased expression of fibrillar collagens. Myofibroblasts correlate with poor overall survival rates in LUAD, associated with loss of epithelial differentiation, TP53 mutations, proximal molecular subtypes and myeloid cell recruitment. In squamous carcinomas myofibroblasts were not prognostic despite being transcriptomically equivalent. These findings have important implications for developing fibroblast-targeting strategies for cancer therapy.
ViralCC retrieves complete viral genomes and virus-host pairs from metagenomic Hi-C data
Authors: Du Yuxuan , Fuhrman Jed A. , Sun Fengzhu
Published: January 2023

The introduction of high-throughput chromosome conformation capture (Hi-C) into metagenomics enables reconstructing high-quality metagenome-assembled genomes (MAGs) from microbial communities. Despite recent advances in recovering eukaryotic, bacterial, and archaeal genomes using Hi-C contact maps, few of Hi-C-based methods are designed to retrieve viral genomes. Here we introduce ViralCC, a publicly available tool to recover complete viral genomes and detect virus-host pairs using Hi-C data. Compared to other Hi-C-based methods, ViralCC leverages the virus-host proximity structure as a complementary information source for the Hi-C interactions. Using mock and real metagenomic Hi-C datasets from several different microbial ecosystems, including the human gut, cow fecal, and wastewater, we demonstrate that ViralCC outperforms existing Hi-C-based binning methods as well as state-of-the-art tools specifically dedicated to metagenomic viral binning. ViralCC can also reveal the taxonomic structure of viruses and virus-host pairs in microbial communities. When applied to a real wastewater metagenomic Hi-C dataset, ViralCC constructs a phage-host network, which is further validated using CRISPR spacer analyses. ViralCC is an open-source pipeline available athttps://github.com/dyxstat/ViralCC.
Dual hydrogen production from electrocatalytic water reduction coupled with formaldehyde oxidation via a copper-silver electrocatalyst
Authors: Li Guodong , Han Guanqun , Wang Lu , Cui Xiaoyu , Moehring Nicole K. , Kidambi Piran R. , Jiang De-en , Sun Yujie
Published: January 2023

The broad employment of water electrolysis for hydrogen (H2) production is restricted by its large voltage requirement and low energy conversion efficiency because of the sluggish oxygen evolution reaction (OER). Herein, we report a strategy to replace OER with a thermodynamically more favorable reaction, the partial oxidation of formaldehyde to formate under alkaline conditions, using a Cu3Ag7electrocatalyst. Such a strategy not only produces more valuable anodic product than O2but also releases H2at the anode with a small voltage input. Density functional theory studies indicate the H2C(OH)O intermediate from formaldehyde hydration can be better stabilized on Cu3Ag7than on Cu or Ag, leading to a lower C-H cleavage barrier. A two-electrode electrolyzer employing an electrocatalyst of Cu3Ag7(+)||Ni3N/Ni(–) can produce H2at both anode and cathode simultaneously with an apparent 200% Faradaic efficiency, reaching a current density of 500 mA/cm2with a cell voltage of only 0.60 V.
COx hydrogenation to methanol and other hydrocarbons under mild conditions with Mo3S4@ZSM-5
Authors: Liu Gui , Liu Pengfei , Meng Deming , Zhao Taotao , Qian Xiaofeng , He Qiang , Guo Xuefeng , Qi Jizhen , Peng Luming , Xue Nianhua , Zhu Yan , Ma Jingyuan , Wang Qiang , Liu Xi , Chen Liwei , Ding Weiping
Published: January 2023

The hydrogenation of CO2 or CO to single organic product has received widespread attentions. Here we show a highly efficient and selective catalyst, Mo3S4@ions-ZSM-5, with molybdenum sulfide clusters ([Mo3S4]n+) confined in zeolitic cages of ZSM-5 molecular sieve for the reactions. Using continuous fixed bed reactor, for CO2 hydrogenation to methanol, the catalyst Mo3S4@NaZSM-5 shows methanol selectivity larger than 98% at 10.2% of carbon dioxide conversion at 180 °C and maintains the catalytic performance without any degeneration during continuous reaction of 1000 h. For CO hydrogenation, the catalyst Mo3S4@HZSM-5 exhibits a selectivity to C2 and C3 hydrocarbons stably larger than 98% in organics at 260 °C. The structure of the catalysts and the mechanism of COx hydrogenation over the catalysts are fully characterized experimentally and theorectically. Based on the results, we envision that the Mo3S4@ions-ZSM-5 catalysts display the importance of active clusters surrounded by permeable materials as mesocatalysts for discovery of new reactions.
Irisin exhibits neuroprotection by preventing mitochondrial damage in Parkinson’s disease
Authors: Zhang Xi , Xu Sutong , Hu Yong , Liu Qiulu , Liu Chenming , Chai Huazhen , Luo Yuping , Jin Lingjing , Li Siguang
Published: January 2023

Exercise has been proposed as an effective non-pharmacological management for Parkinson’s disease (PD) patients. Irisin, a recently identified myokine, is increased by exercise and plays pivotal roles in energy metabolism. However, it remains unknown whether irisin has any protective effects on PD. Here, we found that serum irisin levels of PD patients were markedly elevated after 12-week regular exercise, which had a positive correlation with improved balance function scored by Berg Balance Scale. Treatment with exogenous irisin could improve motor function, and reduce dopaminergic neurodegeneration in PD models. Meanwhile, irisin could reduce cell apoptosis by renovating mitochondrial function in PD models, which was reflected in decreased oxidative stress, increased mitochondrial complex I activity and mitochondrial content, increased mitochondrial biogenesis, and repaired mitochondrial morphology. Furthermore, irisin regulated the aforementioned aspects by upregulating downstream Akt signaling pathway and ERK1/2 signaling pathway through integrin receptors rather than directly targeting mitochondria. With the use of small-molecule inhibitors, it was found that irisin can reduce apoptosis, restore normal mitochondrial biogenesis, and improve mitochondrial morphology and dynamic balance in PD models by activating Akt signaling pathway and ERK1/2 signaling pathway. And irisin reduced oxidative stress via activating ERK1/2 signaling pathway. The results revealed that exogenous irisin conferred neuroprotection relieving apoptosis and oxidative stress, restraining mitochondrial fragmentation, and promoting mitochondrial respiration and biogenesis in PD models, and irisin exerted the aforementioned effects by activating Akt signaling pathway and ERK1/2 signaling pathway. Thus, peripherally delivered irisin might be a promising candidate for therapeutic targeting of PD.
Geostrophic flows control future changes of oceanic eastern boundary upwelling
Authors: Jing Zhao , Wang Shengpeng , Wu Lixin , Wang Hong , Zhou Shenghui , Sun Bingrong , Chen Zhaohui , Ma Xiaohui , Gan Bolan , Yang Haiyuan
Published: January 2023

Equatorward alongshore winds over major eastern boundary upwelling systems (EBUSs) drive intense upwelling via Ekman dynamics, surfacing nutrient-rich deep waters and promoting marine primary production and fisheries. It is generally thought, dating back to Bakun’s hypothesis, that greenhouse warming should enhance upwelling in EBUSs by intensifying upwelling-favourable winds; yet this has not been tested. Here, using an ensemble of high-resolution climate simulations with improved EBUS representation, we show that long-term upwelling changes in EBUSs differ substantially, under a high-emission scenario, from those inferred by the wind-based upwelling index. Specifically, weakened or unchanged upwelling can coincide with intensified upwelling-favourable winds. These differences are linked to long-term changes of geostrophic flows that dominate upwelling changes in the Canary and Benguela currents and strongly offset wind-driven changes in the California and Humboldt currents. Our results highlight the controlling role of geostrophic flows in upwelling trends in EBUSs under greenhouse warming, which Bakun’s hypothesis overlooked.
Forest edges increase pollinator network robustness to extinction with declining area
Authors: Ren Peng , Didham Raphael K. , Murphy Mark V. , Zeng Di , Si Xingfeng , Ding Ping
Published: January 2023

Edge effects often exacerbate the negative effects of habitat loss on biodiversity. In forested ecosystems, however, many pollinators actually prefer open sunny conditions created by edge disturbances. We tested the hypothesis that forest edges have a positive buffering effect on plant-pollinator interaction networks in the face of declining forest area. In a fragmented land-bridge island system, we recorded ~20,000 plant-pollinator interactions on 41 islands over 3 yr. We show that plant richness and floral resources decline with decreasing forest area at both interior and edge sites, but edges maintain 10-fold higher pollinator abundance and richness regardless of area loss. Edge networks contain highly specialized species, with higher nestedness and lower modularity than interior networks, maintaining high robustness to extinction following area loss while forest interior networks collapse. Anthropogenic forest edges benefit community diversity and network robustness to extinction in the absence of natural gap-phase dynamics in small degraded forest remnants.
Chromosome fusions repatterned recombination rate and facilitated reproductive isolation during Pristionchus nematode speciation
Authors: Yoshida Kohta , Rödelsperger Christian , Röseler Waltraud , Riebesell Metta , Sun Simo , Kikuchi Taisei , Sommer Ralf J.
Published: January 2023

Large-scale genome-structural evolution is common in various organisms. Recent developments in speciation genomics revealed the importance of inversions, whereas the role of other genome-structural rearrangements, including chromosome fusions, have not been well characterized. We study genomic divergence and reproductive isolation of closely related nematodes: the androdioecious (hermaphroditic) modelPristionchus pacificusand its dioecious sister speciesPristionchus exspectatus. A chromosome-level genome assembly ofP. exspectatususing single-molecule and Hi-C sequencing revealed a chromosome-wide rearrangement relative toP. pacificus. Strikingly, genomic characterization and cytogenetic studies including outgroup speciesPristionchus occultusindicated two independent fusions involving the same chromosome, ChrIR, between these related species. Genetic linkage analysis indicated that these fusions altered the chromosome-wide pattern of recombination, resulting in large low-recombination regions that probably facilitated the coevolution between some of the ~14.8% of genes across the entire genomes. Quantitative trait locus analyses for hybrid sterility in all three sexes revealed that major quantitative trait loci mapped to the fused chromosome ChrIR. While abnormal chromosome segregations of the fused chromosome partially explain hybrid female sterility, hybrid-specific recombination that breaks linkage of genes in the low-recombination region was associated with hybrid male sterility. Thus, recent chromosome fusions repatterned recombination rate and drove reproductive isolation duringPristionchusspeciation.
Quality control and removal of technical variation of NMR metabolic biomarker data in ~120,000 UK Biobank participants
Authors: Ritchie Scott C. , Surendran Praveen , Karthikeyan Savita , Lambert Samuel A. , Bolton Thomas , Pennells Lisa , Danesh John , Di Angelantonio Emanuele , Butterworth Adam S. , Inouye Michael
Published: January 2023

Metabolic biomarker data quantified by nuclear magnetic resonance (NMR) spectroscopy in approximately 121,000 UK Biobank participants has recently been released as a community resource, comprising absolute concentrations and ratios of 249 circulating metabolites, lipids, and lipoprotein sub-fractions. Here we identify and characterise additional sources of unwanted technical variation influencing individual biomarkers in the data available to download from UK Biobank. These included sample preparation time, shipping plate well, spectrometer batch effects, drift over time within spectrometer, and outlier shipping plates. We developed a procedure for removing this unwanted technical variation, and demonstrate that it increases signal for genetic and epidemiological studies of the NMR metabolic biomarker data in UK Biobank. We subsequently developed an R package, ukbnmr, which we make available to the wider research community to enhance the utility of the UK Biobank NMR metabolic biomarker data and to facilitate rapid analysis.
An RNA-seq time series of the medaka pituitary gland during sexual maturation
Authors: Ager-Wick Eirill , Maugars Gersende , von Krogh Kristine , Fontaine Romain , Weltzien Finn-Arne , Henkel Christiaan
Published: January 2023

Directing both organismal homeostasis and physiological adaptation, the pituitary is a key endocrine gland in all vertebrates. One of its major tasks is to coordinate sexual maturation through the production and release of hormones stimulating gonad development. In order to study its developmental dynamics in the model fish medaka (Oryzias latipes), we sampled both the pituitary and the ovaries of 68 female fish. Of these, 55 spanned the entire course of sexual maturation from prepubertal juveniles to spawning adults. An additional 13 showed either considerably faster or slower growth and development than the majority of fish. We used histological examination of the ovaries to determine a histological maturation stage, and analyzed the pituitary glands using RNA-seq optimized for low input. Taken together, these data reveal the timing of hormone production priorities, and form a comprehensive resource for the study of their regulation.
Investigation of respirable coal mine dust (RCMD) and respirable crystalline silica (RCS) in the U.S. underground and surface coal mines
Authors: Rahimi Elham , Shekarian Younes , Shekarian Naser , Roghanchi Pedram
Published: January 2023

Dust is an inherent byproduct of mining activities that raises notable health and safety concerns. Cumulative inhalation of respirable coal mine dust (RCMD) and respirable crystalline silica (RCS) can lead to obstructive lung diseases. Despite considerable efforts to reduce dust exposure by decreasing the permissible exposure limits (PEL) and improving the monitoring techniques, the rate of mine workers with respiratory diseases is still high. The root causes of the high prevalence of respiratory diseases remain unknown. This study aimed to investigate contributing factors in RCMD and RCS dust concentrations in both surface and underground mines. To this end, a data management approach is performed on MSHA’s database between 1989 and 2018 using SQL data management. In this process, all data were grouped by mine ID, and then, categories of interests were defined to conduct statistical analysis using the generalized estimating equation (GEE) model. The total number of 12,537 and 9050 observations for respirable dust concentration are included, respectively, in the U.S. underground and surface mines. Several variables were defined in four categories of interest including mine type, geographic location, mine size, and coal seam height. Hypotheses were developed for each category based on the research model and were tested using multiple linear regression analysis. The results of the analysis indicate higher RCMD concentration in underground compared to RCS concentration which is found to be relatively higher in surface coal mines. In addition, RCMD concentration is seen to be higher in the Interior region while RCS is higher in the Appalachia region. Moreover, mines of small sizes show lower RCMD and higher RCS concentrations. Finally, thin-seam coal has greater RCMD and RCS concentrations compared to thicker seams in both underground and surface mines. In the end, it is demonstrated that RCMD and RCS concentrations in both surface and underground mines have decreased. Therefore, further research is needed to investigate the efficacy of the current mass-concentration-based monitoring system.
Biodegradable polymer everolimus-eluting stents versus contemporary drug-eluting stents: a systematic review and meta‑analysis
Authors: Yin Juntao , Li Yang , Chen Yangyang , Wang Chaoyang , Song Xiaoyong
Published: January 2023

In spite of similar efficacy and safety in pilot studies, compared with the contemporary durable polymer drug-eluting stent (DP-DES), the bioabsorbable polymer drug-eluting stent (BP-DES) may be more superior in promoting blood vessel healing. We sought to compare the safety and efficacy of everolimus-eluting BP-DES (BP-EES) with contemporary DP-DES through a meta-analysis. We performed this meta-analysis to provide further evidence of the safety and efficacy of BP-EES. Medline, Embase and the Cochrane library databases were searched for randomized controlled trials comparing clinical efficacy and safety of BP-EES versus contemporary DP-DES. Fifteen RCTs with a total of 15,572 patients were selected. The rate of MACE was 9.4% in patients receiving BP-EES and 7.3% receiving DP-EES (RR 1.13, 95% CI 0.99–1.29, p = 0.05; I2 = 46%). TLF and MI were also similar in both groups. Based on the available data, this review demonstrates that BP-EES displays a clinically comparable efficacy and safety profile to that of contemporary DP-DES at years of follow-up in patients undergoing PCI.
Exploring the hub mechanisms of ischemic stroke based on protein-protein interaction networks related to ischemic stroke and inflammatory bowel disease
Authors: Hu Wei , Li Ping , Zeng Nianju , Tan Sheng
Published: January 2023

Ischemic stroke is highly concerning because it often leads to severe long-term neurological disability. Among clinical trials, ischemic stroke and inflammatory bowel disease interactions have been increasingly reported in recent years. Therefore, using bioinformatics approaches to explore novel protein interactions between them is of interest. We performed this exploratory analysis by using bioinformatics tools such as string to analyze gene data downloaded from NHGRI-GWAS data related to ischemic stroke and inflammatory bowel disease. We constructed a prospective protein interaction network for ischemic stroke and inflammatory bowel disease, identifying cytokine and interleukin-related signaling pathways, Spliceosome, Ubiquitin–Proteasome System (UPS), Thrombus, and Anticoagulation pathways as the crucial biological mechanisms of the network. Furthermore, we also used data-independent acquisition mass spectrometry (DIA-MS) to detect differential protein expression in eight samples, which also suggested that immune system, signal transduction, and hemostasis-related pathways are key signaling pathways. These findings may provide a basis for understanding the interaction between these two states and exploring possible molecular and therapeutic studies in the future.
Effect of bismuth oxide nanoparticle on the electromagnetic interference shielding and thermal stability of industrial waste based-geopolymer composites
Authors: Maestre Christian V. , Santos Gil Nonato
Published: January 2023

Gold mine tailings, fly ash, and bagasse ash has been repurposed to produce geopolymer (GP) with enhanced electromagnetic interference shielding efficiency (EMI-SE) and high thermal property. GP has low shielding efficiency compared to concrete. Due to this, an appropriate filler must be incorporated into its matrix to enhance its EMI-SE. For this study, bismuth oxide nanomaterial (BiNP) was utilized as the additive filler. The percent content of BiNP was varied to evaluate its influence on the EMI-SE of GP. Morphology shows that Bi2O3 was embedded in the matrix of GP, and no new aluminu-phyllosilicate minerals were formed. This indicates that some minerals acted only as internal fillers in the matrix. Compressive strength shows synthesized GP composites were more than 20 MPa, with neat GP reaching the maximum strength. Moreover, the EMI-SE of neat GP was 21.2 dB for 20–4500 MHz range. This indicates that GP alone has sufficient characteristics to attenuate EMI radiation. Addition of 5%, 10% and 15% weight of BiNP improves EMI-SE by 4–10%, with 5% BiNP shown to be the optimum ratio. Lastly, the addition of BiNP improves the thermal stability of GP. This study shows that GP incorporated with Bi2O3 can be recommended for small-scale construction and small residential building.
Production of zirconium-88 via proton irradiation of metallic yttrium and preparation of target for neutron transmission measurements at DICER
Authors: Matyskin Artem V. , Stamatopoulos Athanasios , O’Brien Ellen M. , DiGiovine Brad J. , Mocko Veronika , Fassbender Michael E. , Vermeulen C. Etienne , Koehler Paul E.
Published: January 2023

A process for the production of tens to hundreds of GBq amounts of zirconium-88 (88Zr) using proton beams on yttrium was developed. For this purpose, yttrium metal targets (≈20 g) were irradiated in a ~16 to 34 MeV proton beam at a beam current of 100–200 µA at the Los Alamos Isotope Production Facility (IPF). The 88Zr radionuclide was produced and separated from the yttrium targets using hydroxamate resin with an elution yield of 94(5)% (1σ). Liquid DCl solution in D2O was selected as a suitable 88Zr sample matrix due to the high neutron transmission of deuterium compared to hydrogen and an even distribution of 88Zr in the sample matrix. The separated 88Zr was dissolved in DCl and 8 µL of the obtained solution was transferred to a tungsten sample can with a 1.2 mm diameter hole using a syringe and automated filling station inside a hot cell. Neutron transmission of the obtained 88Zr sample was measured at the Device for Indirect Capture Experiments on Radionuclides (DICER).
Food protein-derived amyloids do not accelerate amyloid β aggregation
Authors: Rahman M. Mahafuzur , Pires Rodrigo Sanches , Herneke Anja , Gowda Vasantha , Langton Maud , Biverstål Henrik , Lendel Christofer
Published: January 2023

The deposition of proteins in the form of amyloid fibrils is closely associated with several serious diseases. The events that trigger the conversion from soluble functional proteins into insoluble amyloid are not fully understood. Many proteins that are not associated with disease can form amyloid with similar structural characteristics as the disease-associated fibrils, which highlights the potential risk of cross-seeding of disease amyloid by amyloid-like structures encountered in our surrounding. Of particular interest are common food proteins that can be transformed into amyloid under conditions similar to cooking. We here investigate cross-seeding of amyloid-β (Aβ), a peptide known to form amyloid during the development of Alzheimer’s disease, by 16 types of amyloid fibrils derived from food proteins or peptides. Kinetic studies using thioflavin T fluorescence as output show that none of the investigated protein fibrils accelerates the aggregation of Aβ. In at least two cases (hen egg lysozyme and oat protein isolate) we observe retardation of the aggregation, which appears to originate from interactions between the food protein seeds and Aβ in aggregated form. The results support the view that food-derived amyloid is not a risk factor for development of Aβ pathology and Alzheimer’s disease.
Development and evaluation of deep learning algorithms for assessment of acute burns and the need for surgery
Authors: Boissin Constance , Laflamme Lucie , Fransén Jian , Lundin Mikael , Huss Fredrik , Wallis Lee , Allorto Nikki , Lundin Johan
Published: January 2023

Assessment of burn extent and depth are critical and require very specialized diagnosis. Automated image-based algorithms could assist in performing wound detection and classification. We aimed to develop two deep-learning algorithms that respectively identify burns, and classify whether they require surgery. An additional aim assessed the performances in different Fitzpatrick skin types. Annotated burn (n = 1105) and background (n = 536) images were collected. Using a commercially available platform for deep learning algorithms, two models were trained and validated on 70% of the images and tested on the remaining 30%. Accuracy was measured for each image using the percentage of wound area correctly identified and F1 scores for the wound identifier; and area under the receiver operating characteristic (AUC) curve, sensitivity, and specificity for the wound classifier. The wound identifier algorithm detected an average of 87.2% of the wound areas accurately in the test set. For the wound classifier algorithm, the AUC was 0.885. The wound identifier algorithm was more accurate in patients with darker skin types; the wound classifier was more accurate in patients with lighter skin types. To conclude, image-based algorithms can support the assessment of acute burns with relatively good accuracy although larger and different datasets are needed.
Candidatus Neoehrlichia mikurensis and Hepatozoon sp. in voles (Microtus spp.): occurrence and evidence for vertical transmission
Authors: Tołkacz Katarzyna , Kowalec Maciej , Alsarraf Mohammed , Grzybek Maciej , Dwużnik-Szarek Dorota , Behnke Jerzy M. , Bajer Anna
Published: January 2023

Candidatus Neoehrlichia mikurensis (CNM) and Hepatozoon spp. are important vector-borne parasites of humans and animals. CNM is a relatively recently discovered pathogen of humans. Hepatozoon are parasites of reptiles, amphibians and mammals, commonly found in rodents and carnivores worldwide. The present study aimed to determine the prevalence of CNM and Hepatozoon spp. in three species of Microtus and to assess the occurrence of vertical transmission in naturally-infected voles. Molecular techniques were used to detect pathogen DNA in blood and tissue samples of captured voles and their offspring. The prevalence of CNM in the vole community ranged 24–47% depending on Microtus species. The DNA of CNM was detected in 21% of pups from three litters of six infected Microtus dams (two Microtus arvalis and one M. oeconomus) and in 3/45 embryos (6.6%) from two litters of eight CNM-infected pregnant females. We detected Hepatozoon infection in 14% of M. arvalis and 9% of M. oeconomus voles. Hepatozoon sp. DNA was detected in 48.7% of pups from seven litters (6 M. arvalis and 1 M. oeconomus) and in two embryos (14.3%) obtained from one M. arvalis litter. The high prevalence of CNM infections in the Microtus spp. community may be a result of a relatively high rate of vertical transmission among naturally infected voles. Vertical transmission was also demonstrated for Hepatozoon sp. in M. arvalis and M. oeconomus. Our study underlines the significance of alternative routes of transmission of important vector-borne pathogens.
Accuracy of breathing and radial pulse assessment by non-medical persons: an observational cross-sectional study
Authors: Suzuki Kensuke , Sakaniwa Ryoto , Endo Nobuko , Kubota Miho , Nakazawa Mayumi , Narikawa Kenji , Ogawa Satoo , Yokota Hiroyuki
Published: January 2023

Early recognition of cardiopulmonary arrest (CPA) expedites emergency calls and resuscitation and improves the survival rate of unresponsive individuals. However, the accuracy of breathing and radial artery pulse assessment by non-medical persons is poorly understood. The aim of this study was to determine the accuracy of breathing assessment and radial pulse palpation among 450 non-medical personnel using a high-fidelity simulator. We examined the accuracy of 10 second’s assessment for breathing and radial pulse using a high-fidelity mannequin simulator, included 496 non-medical participants (school teachers) between 2016–2018. For a primary results, the sensitivity for the detection of the presence of the breathing and radial pulse was 96.2% (97.5% for sensitivity and 92.0% for specificity) and 91.7% (99.1% for sensitivity and 56.8% for specificity), respectively. Futher, breathing rate and radial pulse rate were strongly correlated with the assessments, with Spearman’s correlation coefficients of 0.813 (P < 0.001) and 0.719 (P < 0.001), respectively. In contrast, radial pulse strength was weakly correlated with the assessment (coefficient of 0.288, P < 0.001). Our results suggested that non-medical persons would show high accuracy in detecting and measuring respiration and radial pulse, although they did not accurately determine radial pulse strength for the early recognition of CPA.
A novel shape-based approach to identify gestational age-adjusted growth patterns from birth to 11 years of age
Authors: López-Domínguez Lorena , Bassani Diego G. , Bourdon Celine , Massara Paraskevi , Santos Iná S. , Matijasevich Alicia , Barros Aluísio. J. D. , Comelli Elena M. , Bandsma Robert H. J.
Published: January 2023

Child growth patterns assessment is critical to design public health interventions. However, current analytical approaches may overlook population heterogeneity. To overcome this limitation, we developed a growth trajectories clustering pipeline that incorporates a shape-respecting distance, baseline centering (i.e., birth-size normalized trajectories) and Gestational Age (GA)-correction to characterize shape-based child growth patterns. We used data from 3945 children (461 preterm) in the 2004 Pelotas Birth Cohort with at least 3 measurements between birth (included) and 11 years of age. Sex-adjusted weight-, length/height- and body mass index-for-age z-scores were derived at birth, 3 months, and at 1, 2, 4, 6 and 11 years of age (INTERGROWTH-21st and WHO growth standards). Growth trajectories clustering was conducted for each anthropometric index using k-means and a shape-respecting distance, accounting or not for birth size and/or GA-correction. We identified 3 trajectory patterns for each anthropometric index: increasing (High), stable (Middle) and decreasing (Low). Baseline centering resulted in pattern classification that considered early life growth traits. GA-correction increased the intercepts of preterm-born children trajectories, impacting their pattern classification. Incorporating shape-based clustering, baseline centering and GA-correction in growth patterns analysis improves the identification of subgroups meaningful for public health interventions.
An automated micro solid phase extraction gas chromatography–mass spectrometry (μSPE-GC–MS) detection method for geosmin and 2-methylisoborneol in drinking water
Authors: Bristow R. L. , Haworth-Duff A. , Young I. S. , Myers P. , Hampson M. R. , Williams J. , Maher S.
Published: January 2023

Geosmin and 2-methylisoborneol (2-MIB) are amongst the most common earthy and musty taste and odour (T&O) compounds found in drinking water. With low odour threshold detection limits below 10 ng L−1, and the complexity of raw water matrices, these two compounds provide a significant challenge for water companies globally. In this research, for the first time, a novel and fully automated micro-solid phase-extraction (μSPE) method coupled with gas chromatography (GC)–mass spectrometry (MS) has been developed for the detection of geosmin and 2-MIB for drinking water analysis. The new automated method described herein is environmentally friendly requiring low raw water sample volumes, of 25 mL, and only 50 μL of elution solvent. Our μSPE-GC–MS method exhibits excellent linearity for both compounds (R2 > 0.999) and low limits of detection of 2.0 ng L−1and 4.3 ng L−1for geosmin and 2-MIB, respectively. The method showed excellent recovery rates (95.1–100.1%) and good precision (RSD < 7%) in raw sample matrices. Our approach is fully automated onto a robotic workstation which can be readily integrated into a laboratory workflow for routine water analysis. Furthermore, the method has excellent potential to be incorporated within a portable system for onsite analysis.
Estimation of groundwater storage loss for the Indian Ganga Basin using multiple lines of evidence
Authors: Janardhanan Sreekanth , Nair Akhilesh S. , Indu J. , Pagendam Dan , Kaushika G. S.
Published: January 2023

We used remote sensing data, field observations and numerical groundwater modelling to investigate long-term groundwater storage losses in the regional aquifer of the Ganga Basin in India. This comprised trend analysis for groundwater level observations from 2851 monitoring bores, groundwater storage anomaly estimation using GRACE and Global Land Data Assimilation System (GLDAS) data sets and numerical modelling of long-term groundwater storage changes underpinned by over 50,000 groundwater level observations and uncertainty analysis. Three analyses based on different methods consistently informed that groundwater storage in the aquifer is declining at a significant rate. Groundwater level trend indicated storage loss in the range − 1.1 to − 3.3 cm year−1 (median − 2.6 cm year−1) while the modelling and GRACE storage anomaly methods indicated the storage loss in the range of − 2.1 to − 4.5 cm year−1 (median − 3.2 cm year−1) and − 1.0 to − 4.2 cm year−1 (median − 1.7 cm year−1) respectively. Probabilistic modelling analysis also indicated that the average groundwater storage is declining in all the major basin states, the highest declining trend being in the western states of Rajasthan, Haryana and Delhi. While smaller compared to the western states, average groundwater storage in states further towards east—Uttar Pradesh, Bihar and West Bengal within the basin are also declining. Time series of storage anomalies obtained from the three methods showed similar trends. Probabilistic storage analysis using the numerical model vetted by observed trend analysis and GRACE data provides the opportunity for predictive analysis of storage changes for future climate and other scenarios.
Digital twin-based multi-level task rescheduling for robotic assembly line
Authors: Yao Bitao , Xu Wenjun , Shen Tong , Ye Xun , Tian Sisi
Published: January 2023

Assembly is a critical step in the manufacturing process. Robotic assembly technology in automatic production lines has greatly improved the production efficiency. However, in assembly process, dynamic disturbances such as processing time change and advance delivery may occur, which cause the scheduling deviation. Traditional scheduling methods are not sufficient to meet the real-time and adaptive requirements in smart manufacturing. Digital twin (DT) has the characteristics of virtual-reality interaction and real-time mapping. In this paper, we propose a DT-based framework of task rescheduling for robotic assembly line (RAL) and its key methodologies, thus to realize the timely and dynamic adjustment of scheduling plan under uncertain interferences. First, a DT model of RAL task rescheduling composed of physical entity (PE), virtual entity (VE), and virtual-reality interaction mechanism is proposed. Then, a mathematical model is established. By analyzing the adaptive objective thresholds from the perspectives of event trigger and user demand trigger, a DT-driven multi-level (production unit level and line level) rescheduling strategy is proposed. Taking both the computing time and solution quality into consideration, the precedence graph is introduced to propose a rescheduling approach based on an improved discrete fireworks algorithm. Finally, the effectiveness of the proposed model and approach are verified by task scheduling experiments of RAL.