bluuebunny/crossref_metadata_2025 huggingface dataset:
Implications and Emerging Therapeutic Avenues of Inflammatory Response in HPV+ Head and Neck Squamous Cell Carcinoma
Authors: Castellano Lúcio Roberto Cançado , Cruz Sara Brito Silva Costa , Hier Michael , Bonan Paulo Rogério Ferreti , Alaoui-Jamali Moulay A. , da Silva Sabrina Daniela
Published: November 2022

Head and neck squamous cell carcinomas (HNSCC) are a heterogeneous group of malignancies which have shown exponential incidence in the last two decades especially due to human papillomavirus (HPV) infection. The HPV family comprises more than 100 types of viruses with HPV16 and HPV18 being the most prevalent strains in HNSCC. Literature data reveal that the mutation profile as well as the response to chemotherapy and radiotherapy are distinct among HPV+ versus HPV-negative tumors. Furthermore, the presence of the virus induces activation of an immune response, in particular the recruitment of specific antiviral T lymphocytes to tumor sites. These T cells when activated produce soluble factors including cytokines and chemokines capable of modifying the local immune tumor microenvironment and impact on tumor response to the treatment. In this comprehensive review we investigated current knowledge on how the presence of an HPV can modify the inflammatory response systemically and within the tumor microenvironment’s immunological responses, thereby impacting on disease prognosis and survival. We highlighted the research gaps and emerging approaches necessary to discover novel immunotherapeutic targets for HPV-associated HNSCC.
A Novel Potential Role for Monocytes Revealed by Single Cell Analysis of Immunotherapy Induced Immune Related Adverse Events
Authors: Garrison Zachary , Chang Matthew , Hornick Noah , Yu Wesley Y. , Cheng Jeffrey B. , Kulkarni Rajan P.
Published: November 2022

Immune related adverse events (irAEs) are one of the leading causes of discontinuation of cancer immunotherapy treatment. Despite extensive research into the frequency and types of irAEs, little is known about the cell types and pathways through which these drugs cause the observed side effects. To identify cell types and pathways of interest, we have analyzed single cell sequencing data of PBMCs from patients who developed skin irAEs as a result of their immunotherapy treatment. Using Azimuth’s cell type identification software for PBMCs and GSEA pathway analysis, we found macrophage cell populations and reactive oxygen species related pathways to be upregulated. These results provide important groundwork to build a complete picture of the mechanisms which cause irAEs and finding ways to more effectively treat them.
Optimization of Transpedicular Electrode Insertion for Electroporation-Based Treatments of Vertebral Tumors
Authors: Cindrič Helena , Miklavčič Damijan , Cornelis Francois H. , Kos Bor
Published: November 2022

Electroporation-based treatments such as electrochemotherapy and irreversible electroporation ablation have sparked interest with respect to their use in medicine. Treatment planning involves determining the best possible electrode positions and voltage amplitudes to ensure treatment of the entire clinical target volume (CTV). This process is mainly performed manually or with computationally intensive genetic algorithms. In this study, an algorithm was developed to optimize electrode positions for the electrochemotherapy of vertebral tumors without using computationally intensive methods. The algorithm considers the electric field distribution in the CTV, identifies undertreated areas, and uses this information to iteratively shift the electrodes from their initial positions to cover the entire CTV. The algorithm performs successfully for different spinal segments, tumor sizes, and positions within the vertebra. The average optimization time was 71 s with an average of 4.9 iterations performed. The algorithm significantly reduces the time and expertise required to create a treatment plan for vertebral tumors. This study serves as a proof of concept that electrode positions can be determined (semi-)automatically based on the spatial information of the electric field distribution in the target tissue. The algorithm is currently designed for the electrochemotherapy of vertebral tumors via a transpedicular approach but could be adapted for other anatomic sites in the future.
Stability Studies and the In Vitro Leishmanicidal Activity of Hyaluronic Acid-Based Nanoemulsion Containing Pterodon pubescens Benth. Oil
Authors: Kleinubing Sirlene Adriana , Outuki Priscila Miyuki , Santos Éverton da Silva , Hoscheid Jaqueline , Tominc Getulio Capello , Dalmagro Mariana , Silva Edson Antônio da , Lima Marli Miriam de Souza , Nakamura Celso Vataru , Cardoso Mara Lane Carvalho
Published: November 2022

The physicochemical and microbiological stability of a hyaluronic acid-based nanostructured topical delivery system containing P. pubescens fruit oil was evaluated, and the in vitro antileishmanial activity of the nanoemulsion against Leishmania amazonensis and the cytotoxicity on macrophages was investigated. The formulation stored at 5 ± 2 °C, compared with the formulation stored at 30 and 40 ± 2 °C, showed a higher chemical and physical stability during the period analyzed and in the accelerated physical stability study. The formulation stored at 40 °C presented a significant change in droplet diameter, polydispersity index, zeta potential, pH, active compound, and consistency index and was considered unstable. The microbiological stability of the formulations was confirmed. The leishmanicidal activity of the selected system against intracellular amastigotes was significantly superior to that observed for the free oil. However, further research is needed to explore the use of the hyaluronic acid-based nanostructured system containing P. pubescens fruit oil for the treatment of cutaneous leishmaniasis.
Morphological and Molecular Characterization Using Genitalia and CoxI Barcode Sequence Analysis of Afrotropical Mosquitoes with Arbovirus Vector Potential
Authors: Montalvo-Sabino Eddyson , Abílio Ana Paula , Guarido Milehna Mara , Valadas Vera , Novo Maria Teresa , Kampango Ayubo , Sousa Carla Alexandra , Fafetine José , Venter Marietjie , Thompson Peter N. , Braack Leo , Cornel Anthony John , Parreira Ricardo , de Almeida António Paulo Gouveia
Published: November 2022

Potential arboviral Afrotropical mosquito vectors are underrepresented in public databases of CoxI barcode sequences. Furthermore, available CoxI sequences for many species are often not associated with voucher specimens to match the corresponding fine morphological characterization of specimens. Hence, this study focused on the characterization of Culicine mosquitoes from South Africa, Mozambique, and Angola and their classification using a complementary approach including a morphological analysis of specimens’ genitalia and phylogenetic study based on the analysis of CoxI barcode sequences using maximum likelihood and Bayesian phylogenetic inference methods, alongside Median-Joining Network and PCOORD analyses. Overall, 800 mosquitoes (652 males and 148 females) from 67 species, were analyzed. Genitalia from 663 specimens allowed the identification of 55 species of 10 genera. A total of 247 CoxI partial gene sequences corresponding to 65 species were obtained, 11 of which (Aedes capensis, Ae. mucidus, Culex andersoni, Cx. telesilla, Cx. inconspicuosus, Eretmapodites subsimplicipes, Er. quinquevittatus, Ficalbia uniformis, Mimomyia hispida, Uranotaenia alboabdominalis, and Ur. mashonaensis) are, to the best of our knowledge, provided here for the first time. The presence of Cx. pipiens ecotypes molestus and pipiens and their hybrids, as well as Cx. infula, is newly reported in the Afrotropical region. The rates of correct sequence identification using BOLD and BLASTn (≥95% identity) were 64% and 53%, respectively. Phylogenetic analysis revealed that, except for subgenus Eumelanomyia of Culex, there was support for tribes Aedini, Culicini, Ficalbiini, and Mansoniini. A divergence >2% was observed in conspecific sequences, e.g., Aedeomyia africana, Ae. cumminsii, Ae. unilineatus, Ae. metallicus, Ae. furcifer, Ae. caballus, and Mansonia uniformis. Conversely, sequences from groups and species complexes, namely, Ae. simpsoni, Ae. mcintoshi, Cx. bitaeniorhynchus, Cx. simpsoni, and Cx. pipiens were insufficiently separated. A contribution has been made to the barcode library of Afrotropical mosquitoes with associated genitalia morphological identifications.
Effect of Polytomy on the Parameter Estimation and Goodness of Fit of Phylogenetic Linear Regression Models for Trait Evolution
Authors: Jhwueng Dwueng-Chwuan , Liu Feng-Chi
Published: November 2022

Phylogenetic regression models for trait evolution incorporate phylogenetic trees for the analysis of comparative data, in order to study trait relationships among a group of related species. However, as such trees are estimated, it is unlikely that there will be no errors when describing the relationships among species. In particular, for polytomy trees, where the relationships within a particular clade of species are more/less certainly determined (hard/soft polytomy, respectively), results of comparative analyses obtained from models based on those phylogenetic trees may also be affected. In this study, through extensive simulations, the performances of several popular Gaussian process-based regression models (Brownian motion, BM; Ornstein–Uhlenbeck process, OU; early burst, EB), as well as branch-stretching models (Pagel’s λ,δ,κ), were evaluated by assessing their fit and parameter estimation performance when soft polytomies are presented on either the root or a clade with insufficient phylogenetic information. Comparisons of the models are conducted by either assessing the accuracy of the estimator of regression and model parameters, or using a measure of fit (AIC, r2, and mean square error). It is found that, although polytomy does not significantly impact the fit and parameter estimate within a specified model, distinguishable differences and effects may be observed among trees and models. In particular, Pagel λ model and the OU model yield more accurate estimates and provide better fitting effects than the other models (BM, EB, δ, κ). While correcting phylogeny is an essential step prior to analysis, users may also consider using more appropriate models when encountering the polytomy issue.
Augmentation and Evaluation of an Olive Oil Based Polyherbal Combination against Diabetic Cardiomyopathy in Experimental Model of Rodents
Authors: Shamim Arshiya , Siddiqui Hefazat H. , Mahmood Tarique , Wani Tanveer A. , Zargar Seema , Siddiqui Mohammad Haris , Farooqui Alvina , Ahsan Farogh , Shariq Mohammad , Parveen Saba , Wahajuddin Muhammad , Wal Pranay , Ved Akash
Published: November 2022

Diabetes mellitus is a metabolic disorder that is prima facie a cause for numerous macro and micro vascular complications. A common macroscopic complication associated with diabetes is cardiomyopathy. Cardiomyopathy refers to diseases of the heart muscle, where the heart muscle becomes enlarged, thick, or rigid. As cardiomyopathy worsens, the heart becomes weaker and is unable to conduct the right amount of blood through the body and maintain a normal electrical rhythm. This can lead to heart failure or arrhythmias. Chronic diabetes is one of the instigating factors behind the etiology of this cardiac complication. Type-II diabetes is associated with impaired glucose metabolism that increases the dependence of a diabetic heart on fatty acid oxidation to meet its functional demands, resulting in mitochondrial uncoupling, glucotoxicity, lipotoxicity and initially subclinical cardiac dysfunction that finally gives way to heart failure. The increasing diabetic population with cardiac disorders and the ironically decreasing trend in newer medications to counter this complication leave us at a crossroads for pharmacological management of diabetic cardiomyopathy. Keeping this in view, the present study proclaims a newly developed polyherbal combination (PHC) with three herbs, namely Tinospora cordifolia, Withania somnifera and Boerhavia diffusa based in olive oil and administered in fixed dose (PHC-6 and PHC-10) to screen its cardioprotective potential against a well-established experimental model for diabetic cardiomyopathy. The three herbs mentioned have been known through the traditional literature for their antidiabetic and cardioprotective roles, hence they became the obvious choice. The study follows an experimental model proposed by Reed et al., where the capacity of the β-cell is unobtrusively impeded without totally compromising insulin release, bringing about a moderate disability in glucose resilience. Various sophisticated parameters, namely intraventricular septum thickness of hearts, Western blot of α/β- MHC monoclonal antibody (Ab), cardiac pyruvate dehydrogenase (PDH) activity, medium chain acyl coenzyme A dehydrogenase (MCAD) enzyme, etc. showed promising results where treatment with PHC (PHC-6 and PHC-10) significantly (*** p < 0.001 and **** p < 0.0001) prevented the symptoms of cardiomyopathy in subsequent groups when compared to disease control group.
On Applicability of Quantum Formalism to Model Decision Making: Can Cognitive Signaling Be Compatible with Quantum Theory?
Authors: Khrennikov Andrei
Published: November 2022

This note is devoted to the problem of signaling (marginal inconsistency) in the Bell-type experiments with physical and cognitive systems. It seems that in quantum physics, this problem is still not taken seriously. Only recently have experimenters started to check the signaling hypothesis for their data. For cognitive systems, signaling was statistically significant in all experiments (typically for decision making) performed up to today. Here, one cannot simply ignore this problem. Since signaling contradicts the quantum theory of measurement for compatible observables, its statistical significance in experiments with humans can be considered as an objection for quantum-like modeling—applications of quantum theory to cognition, decision making, psychology, economics and finance, social and political science. In this paper, we point to two possible sources of signaling generation that are consistent with quantum measurement theory. Thus, the signaling objection for quantum-like modeling is not catastrophic. One of these sources is the direct physical signaling about selection of experimental settings, questions or tasks in quantum-like studies. Another possible source is a state modification dependent on experimental settings. The latter was a rather common source of signaling in quantum physics. Since the physical size of the brain is very small comparing with the light velocity, it seems to be impossible to prevent the direct physical signaling (with electromagnetic waves) between the brain’s areas processing two questions a and b. However, if, for these questions, not the electromagnetic waves, but electrochemical communication plays the crucial role, the experimenter may hope to make signaling weaker by answering the questions faster. The problem of question-dependent mental state modification seems to be solvable via smarter experimental design. This paper can be useful both for physicists interested in quantum foundations and for researchers working in quantum-like studies, e.g., applying the quantum theory to model decision making or psychological effects. This paper is solely about quantum theory. Thus, we do not consider general contextual probabilistic models.
Albic Podzols of Boreal Pine Forests of Russia: Soil Organic Matter, Physicochemical and Microbiological Properties across Pyrogenic History
Authors: Dymov Alexey A. , Grodnitskaya Irina D. , Yakovleva Evgenia V. , Dubrovskiy Yuri A. , Kutyavin Ivan N. , Startsev Viktor V. , Milanovsky Evgeni Yu. , Prokushkin Anatoly S.
Published: November 2022

Albic podzols under pine forests are more prone to fires on the planet. The influence of fire extends to all soil components, including chemical properties, microbiological characteristics, and the composition and structure of soil organic matter, which persists for a long time. Here, we present the results of a study of the morphological, physicochemical, and microbiological properties and features of soil organic matter (SOM) in the albic podzols of pine forests (Pinus sylvestris L.) not exposed to fires for a long time (from 45 to 131 years). The study areas are characterized by different numbers of old fires (from four to five) that occurred over the previous several centuries in various territories of the Russian Federation such as Central Siberia (CS) and the European North (EN). In general, the albic podzols developing in CS and EN are characterized by similar morphological and physicochemical properties, with high acidity and poor mineral horizons. In terms of the lower vegetation layer and stand parameters, forest communities at the CS sites have a lower density and species diversity than those in EN. The ground cover is almost completely restored 45 years after the surface fire. The upper mineral horizon of albic podzols in EN contains higher PAHs in comparison with similar horizons of the CS sites. In the soil of EN pine forests, the MB content in the mineral horizons is, on average, three times higher than those in CS. Differences were also found in the qualitative composition of the studied soils’ microbiomes. The EN soil communities are represented by a wide variety of bacteria and fungi. The presented soil parameters can be used as a reference in assessing the increasing impact of fires on pine forests and podzols.
Evaluation of Anaesthetic Effect of Commercial Basil Ocimum basilicum on Zebrafish (Danio rerio) Embryos
Authors: Capparucci Fabiano , De Benedetto Giovanni , Natale Sabrina , Pecoraro Roberta , Iaria Carmelo , Marino Fabio
Published: November 2022

Essential oils (EOs) are natural compounds used in various fields such as traditional medicine, gastronomy, and agriculture, often used against plant and animal parasites. In the present study, the toxic and pathological effects of basil (Ocimum basilicum) essential oil on the development of zebrafish embryos was investigated. The manufacturer datasheet reported linalool and eugenol as major compounds. The effects of the essential oil were evaluated through a zebrafish embryo toxicity test (ZFET) following the OECD n°236 guidelines. Embryos were exposed to different essential oil concentrations (50, 100, and 200 μL/L). Results showed mortality effects in the experimental groups in a dose-dependent manner. Moreover, zebrafish exposed to the higher concentration showed several pathological alterations; particularly, delayed hatching, pericardial edema, and a significant reduction of heart rate after 96 h post-fertilization (hpf) compared to the control group. Results reported here suggest an anaesthetic effect of the O. basilicum at low concentrations, due to the possible synergist effect of the main components such as linalool and eugenol which act on the GABAergic system evoking bradycardia.
Radiation-Induced Intestinal Normal Tissue Toxicity: Implications for Altered Proteome Profile
Authors: Larrey Enoch K. , Pathak Rupak
Published: November 2022

Radiation-induced toxicity to healthy/normal intestinal tissues, especially during radiotherapy, limits the radiation dose necessary to effectively eradicate tumors of the abdomen and pelvis. Although the pathogenesis of intestinal radiation toxicity is highly complex, understanding post-irradiation alterations in protein profiles can provide crucial insights that make radiotherapy safer and more efficient and allow for increasing the radiation dose during cancer treatment. Recent preclinical and clinical studies have advanced our current understanding of the molecular changes associated with radiation-induced intestinal damage by assessing changes in protein expression with mass spectrometry-based approaches and 2-dimensional difference gel electrophoresis. Studies by various groups have demonstrated that proteins that are involved in the inflammatory response, the apoptotic pathway, reactive oxygen species scavenging, and cell proliferation can be targeted to develop effective radiation countermeasures. Moreover, altered protein profiles serve as a crucial biomarkers for intestinal radiation damage. In this review, we present alterations in protein signatures following intestinal radiation damage as detected by proteomics approaches in preclinical and clinical models with the aim of providing a better understanding of how to accomplish intestinal protection against radiation damage.
A Pilot Study on the Prediction of Non-Contact Muscle Injuries Based on ACTN3 R577X and ACE I/D Polymorphisms in Professional Soccer Athletes
Authors: de Almeida Kathleen Y. , Cetolin Tiago , Marrero Andrea Rita , Aguiar Junior Aderbal Silva , Mohr Pedro , Kikuchi Naoki
Published: November 2022

Muscle injuries are among the main reasons for medical leavings of soccer athletes, being a major concern within professional teams and their prevention associated with sport success. Several factors are associated with a greater predisposition to injury, and genetic background is increasingly being investigated. The aim of this study was to analyze whether ACTN3 R577X and ACE I/D polymorphisms are predictors of the incidence and severity of muscle injury in professional soccer athletes from Brazil, individually and in association. Eighty-three professional athletes from the first and second divisions of the Brazilian Championship were evaluated regarding the polymorphisms through blood samples. Nighty-nine muscle injuries were identified during the seasons of 2018, 2019 and 2020 and categorized according to severity. ACTN3 XX individuals had a higher frequency of severe injuries compared to the RX and RR genotypes (p = 0.001), and in the dominant model (compared to RX+RR), with p < 0.001. The trend p-value test showed an increased number of injuries/season following the order XX > RX > RR (p = 0.045). Those with the ACE II genotype had almost 2 fold the number of injuries per season compared to those with the ID+DD genotypes (p = 0.03). Logistic regression showed that the polymorphisms are predictors of the development of severe injury (ACTN3 R577X model with p = 0.004, R2: 0.259; ACE I/D model with p = 0.045, R2: 0.163), where ACTN3 XX individuals were more likely to suffer from severe injury (OR: 5.141, 95% CI: 1.472–17.961, p = 0.010). The combination of the ACTN3 577X allele and the ACE II genotype showed an increased number of injuries per season, enhanced by 100% (1.682 injuries/season versus 0.868 injuries/season, p = 0.016). Our findings suggest that both polymorphisms ACTN3 R577X and ACE I/D (and their interaction) are associated with the susceptibility and severity of non-contact muscle injury in soccer players.
Analysis of Heat Shock Proteins Based on Amino Acids for the Tomato Genome
Authors: Almutairi Meshal M. , Almotairy Hany M.
Published: November 2022

This research aimed to investigate heat shock proteins in the tomato genome through the analysis of amino acids. The highest length among sequences was found in seq19 with 3534 base pairs. This seq19 was reported and contained a family of proteins known as HsfA that have a domain of transcriptional activation for tolerance to heat and other abiotic stresses. The values of the codon adaptation index (CAI) ranged from 0.80 in Seq19 to 0.65 in Seq10, based on the mRNA of heat shock proteins for tomatoes. Asparagine (AAT, AAC), aspartic acid (GAT, GAC), phenylalanine (TTT, TTC), and tyrosine (TAT, TAC) have relative synonymous codon usage (RSCU) values bigger than 0.5. In modified relative codon bias (MRCBS), the high gene expressions of the amino acids under heat stress were histidine, tryptophan, asparagine, aspartic acid, lysine, phenylalanine, isoleucine, cysteine, and threonine. RSCU values that were less than 0.5 were considered rare codons that affected the rate of translation, and thus selection could be effective by reducing the frequency of expressed genes under heat stress. The normal distribution of RSCU shows about 68% of the values drawn from the standard normal distribution were within 0.22 and −0.22 standard deviations that tend to cluster around the mean. The most critical component based on principal component analysis (PCA) was the RSCU. These findings would help plant breeders in the development of growth habits for tomatoes during breeding programs.
Strategies for Bottlenecks of rAAV-Mediated Expression in Skeletal and Cardiac Muscle of Duchenne Muscular Dystrophy
Authors: Li Na , Song Yafeng
Published: November 2022

Gene therapy using the adeno-associated virus (rAAV) to deliver mini/micro- dystrophin is the current promising strategy for Duchenne Muscular Dystrophy (DMD). However, the further transformation of this strategy still faces many “bottlenecks”. Most gene therapies are only suitable for infants with strong muscle cell regeneration and immature immune system, and the treatment depends heavily on the high dose of rAAV. However, high-dose rAAV inevitably causes side effects such as immune response and acute liver toxicity. Therefore, how to reduce the degree of fibrosis and excessive immune response in older patients and uncouple the dependence association between therapeutic effect and high dose rAAV are crucial steps for the transformation of rAAV-based gene therapy. The article analyzes the latest research and finds that the application of utrophin, the homologous protein of dystrophin, could avoid the immune response associated with dystrophin, and the exploration of methods to improve the expression level of mini/micro-utrophin in striated muscle, combined with the novel MyoAAV with high efficiency and specific infection of striated muscle, is expected to achieve the same therapeutic efficacy under the condition of reducing the dose of rAAV. Furthermore, the delivery of allogeneic cardio sphere-derived cells (CDCs) with anti-inflammatory and anti-fibrotic characteristics combined with immune suppression can provide a continuous and appropriate “window period” for gene therapy. This strategy can expand the number of patients who could benefit from gene therapy.
Chemical Composition, Sources, and Health Risk Assessment of PM2.5 and PM10 in Urban Sites of Bangkok, Thailand
Authors: Ahmad Mushtaq , Manjantrarat Thanaphum , Rattanawongsa Wachiraya , Muensri Phitchaya , Saenmuangchin Rattaporn , Klamchuen Annop , Aueviriyavit Sasitorn , Sukrak Kanokwan , Kangwansupamonkon Wiyong , Panyametheekul Sirima
Published: November 2022

Of late, air pollution in Asia has increased, particularly in built-up areas due to rapid industrialization and urbanization. The present study sets out to examine the impact that pollution can have on the health of people living in the inner city of Bangkok, Thailand. Consequently, in 2021, fine particulate matter (PM2.5) and coarse particulate matter (PM10) chemical composition and sources are evaluated at three locations in Bangkok. To identify the possible sources of such particulates, therefore, the principal component analysis (PCA) technique is duly carried out. As determined via PCA, the major sources of air pollution in Bangkok are local emission sources and sea salt. The most significant local sources of PM2.5 and PM10 in Bangkok include primary combustion, such as vehicle emissions, coal combustion, biomass burning, secondary aerosol formation, industrial emissions, and dust sources. Except for the hazard quotient (HQ) of Ni and Mn of PM2.5 for adults, the HQ values of As, Cd, Cr, Mn, and Ni of both PM2.5 and PM10 were below the safe level (HQ = 1) for adults and children. This indicates that exposure to these metals would have non-carcinogenic health effects. Except for the carcinogenic risk (HI) value of Cr of PM2.5 and PM10, which can cause cancer in adults, at Bangna and Din Daeng, the HI values of Cd, Ni, As, and Pb of PM2.5 and PM10 are below the limit set by the U.S. Environmental Protection Agency (U.S. EPA). Ni and Mn pose non-carcinogenic risks, whereas Cr poses carcinogenic risks to adults via inhalation, a serious threat to the residents of Bangkok.
The Carbon Emissions Trading Policy of China: Does It Really Promote the Enterprises’ Green Technology Innovations?
Authors: Li Xiaoqi , Guo Dingfei , Feng Chao
Published: November 2022

The carbon emissions trading policy has profound impacts on the production and operation of enterprises. The aim of this study is to examine the effects of the carbon emissions trading policy on enterprises’ green technology innovations by using PSM−DID models. The results showed that: (1) the carbon emissions trading policy has a facilitating effect on green technology innovation of China’s enterprises in pilot cities; (2) there is significant spatial heterogeneity in this effect and it is extremely beneficial to enterprises’ green technology innovations in eastern China; and (3) the trading policy is proved to have significant positive effects on green technology innovations of non-state and non-high-tech enterprises, while it has no effects on that of state-owned and high-tech enterprises. The above findings were corroborated by the placebo test and other methods.
Dorsolateral Prefrontal Activation in Emotional Autobiographical Task in Depressed and Anxious College Students: An fNIRS Study
Authors: Zhang Yan , Li Xiaoqin , Guo Ying , Zhang Zhe , Xu Fang , Xiang Nian , Qiu Min , Xiao Qiang , Wang Pu , Shi Hui
Published: November 2022

Objective: The dorsolateral prefrontal cortex (dlPFC) is strongly associated with mood symptoms. This study used functional near-infrared spectroscopy (fNIRS) technology to explore the features of brain neural activity in the dlPFC of anxious and depressed college students, during an emotional autobiographical memory task, and to understand the differences in brain cognitive mechanisms caused by anxiety and depression. Methods: A simple random sampling method was used to test 440 college students at a university with a healthy control group (HC, 220 participants), a pure depression group (PD, 92 participants), and a pure anxiety group (PA, 128 participants). The average oxyhemoglobin in the dlPFC of the subjects during the emotional autobiographical memory task was collected by a 53-channel functional near-infrared spectroscopy imaging device. Results: The activation of the left dlPFC (ch13) in the pure depression group was significantly higher than in the pure anxiety group. The activation of the right dlPFC (ch48) was significantly higher under positive emotions than under negative emotions. The interaction between emotion valence and group was marginally significant, and the activation of the right dlPFC (ch41) in the pure depression group was significantly higher under positive emotion than in negative emotion. The activation of the pure depression group under positive emotions was significantly higher than that of the pure anxiety group. In comparison, the activation of the pure depression group under negative emotions was significantly lower than that of the healthy control group. The results of correlation analysis showed that the activation of the left dlPFC (ch13) was significantly negatively correlated with anxiety in positive emotions, but the activation of the right dlPFC (ch34, ch42) was significantly positively correlated with anxiety in positive and negative emotions. Conclusions: The right dlPFC was insensitive to positive emotions in college students with high-anxiety symptoms, whereas this region was insensitive to negative emotions in college students with high depressive symptoms, which might be one of the critical differences in the cognitive mechanisms of anxiety and depression. Furthermore, left and right dlPFC activation correlated differently with anxiety. The higher the anxiety level, the lower the activation on the left side, and the higher the activation on the right side. The results suggested that anxiety might reduce the function of the left dlPFC.
Nigerian Muslim’s Perceptions of Changes in Diet, Weight, and Health Status during Ramadan: A Nationwide Cross-Sectional Study
Authors: Sulaiman Sahabi Kabir , Tsiga-Ahmed Fatimah Isma’il , Faris MoezAlIslam E. , Musa Muhammad Sale , Akpan Udoyen Abasi-okot , Umar Abdullahi Muhammad , Abubakar Salisu Maiwada , Allaham Kholoud K. , Alyammahi Taif , Abdbuljalil Munther A. , Javaid Syed Fahad , Khan Moien AB
Published: November 2022

The Islamic month of Ramadan is marked by fasting, when Muslims refrain from eating and drinking from dawn to sunset, which has an impact on their dietary habits. The study aimed to assess Nigerian Muslims’ dietary modifications during Ramadan and their related changes in body weight and health status. A web-based cross-sectional study was conducted among Nigerian adult Muslims. The survey assessed sociodemographic, dietary habits, eating behaviors, food choices, perceived weight changes, and health status. The logistic regression model was used to assess the predictors of weight change and perceived health status. There were 770 participants, 62.9% of whom were women, ranging in age from 18 to 60 years with a mean age of 27.7 ± 6.4 years. Fruits, palm dates, homemade foods, milk products, and vegetables were more frequently consumed. There were fewer energy drinks, pastries, salty snacks, and carbonated or sugared drinks consumed during Ramadan than before. Over half (54.6%, 95% CI: 51.0–58.9%) of the respondents lost weight during Ramadan, 37.0% (95% CI: 17.4–38.6%) maintained their weight and 8.4% (95% CI: 6.6–10.6%) gained weight during the month. Nearly all (97.3%, 95% CI: 95.8–98.3%) reported having good health during Ramadan, and 2.7% (95% CI: 1.7–4.1%) reported having a poorer health state during Ramadan. There was a significant weight loss and healthy dietary change associated with Ramadan fasting in Nigeria. Public health measures must be in place to impart such positive health behaviors so that such healthy habits continue throughout the year.
Validation Parameters of the Magnetic Stirrer Method for Pooled Sample Digestion for Trichinella spp. in Horse Meat Based on Proficiency Tests Results
Authors: Różycki Mirosław , Korpysa-Dzirba Weronika , Bełcik Aneta , Bilska-Zając Ewa , Gontarczyk Aneta , Kochanowski Maciej , Samorek-Pieróg Małgorzata , Karamon Jacek , Rubiola Selene , Chiesa Francesco , Cencek Tomasz
Published: November 2022

Meat of horses may be infested with nematodes of the genus Trichinella spp. and can cause serious disease in humans. Rules for the carcasses sampling of species susceptible to Trichinella spp. infection and examination are laid down in Commission Regulation 1375/2015, where the magnetic stirrer method for pooled-sample digestion is recommended (Commission Regulation 1478/2020). All personnel involved in the examination should be properly trained and participate in quality control programs. Proficiency tests (PTs) play a key role in the quality verification process. This paper presents the results of PTs organized for 68 Polish laboratories in 2014–2019. Results were assessed qualitatively at three levels of sample contamination (0, 3, 5 larvae) and quantitatively at one level (5 larvae). The laboratories have achieved the average correct qualitative results 100%, 96.2% and 96.8% for the samples contaminated with 0, 3 and 5 larvae, respectively. In the quantitative evaluation, an average 94.1% of the reported results were correct. The data from PTs enabled us to define, for the first time, validation parameters of the digestion method for the horse meat matrix in a large-scale experiment including: specificity (100%), sensitivity (95.6%), accuracy (97.1%), the limit of detection (LOD) (1.14 ≈ 1) and the limit of quantification (LOQ) (3.42 ≈ 3).
Opioid-Free Anaesthesia Effectiveness in Thoracic Surgery—Objective Measurement with a Skin Conductance Algesimeter: A Randomized Controlled Trial
Authors: Sadowska Dominika , Bialka Szymon , Palaczynski Piotr , Czyzewski Damian , Smereka Jacek , Szelka-Urbanczyk Anna , Misiolek Hanna
Published: November 2022

Background: Chest surgery is associated with significant pain, and potent opioid medications are the primary medications used for pain relief. Opioid-free anaesthesia (OFA) combined with regional anaesthesia is promoted as an alternative in patients with an opioid contraindication. Methods: Objective: To assess the efficacy of OFA combined with a paravertebral block in pain treatment during video-assisted thoracic surgery. Design: A randomized, open-label study. Setting: A single university hospital between December 2015 and March 2018. Participants: Sixty-six patients scheduled for elective video-assisted thoracic surgery were randomized into two groups. Of these, 16 were subsequently excluded from the analysis. Interventions: OFA combined with a paravertebral block with 0.5% bupivacaine in the OFA group; typical general anaesthesia with opioids in the control group. Main outcome measures: Intraoperative nociceptive intensity measured with a skin conductance algesimeter (SCA) and traditional intraoperative monitoring. Results: Higher mean blood pressure was observed in the control group before induction and during intubation (p = 0.0189 and p = 0.0095). During chest opening and pleural drainage, higher SCA indications were obtained in the control group (p = 0.0036 and p = 0.0253), while in the OFA group, the SCA values were higher during intubation (p = 0.0325). SCA during surgery showed more stable values in the OFA group. Pearson analysis revealed a positive correlation between the SCA indications and mean blood pressure in both groups. Conclusions: OFA combined with a paravertebral block provides effective nociception control during video-assisted thoracic surgery and can be an alternative for general anaesthesia with opioids. OFA provides a stable nociception response during general anaesthesia, as measured by SCA.
Spatio-Temporal Variability of Malaria Incidence in the Health District of Kati, Mali, 2015–2019
Authors: Katile Abdoulaye , Sagara Issaka , Cissoko Mady , Bationo Cedric Stephane , Dolo Mathias , Thera Ismaila , Traore Siriman , Kone Mamady , Dembele Pascal , Bocoum Djoouro , Sidibe Ibrahima , Simaga Ismael , Sissoko Mahamadou Soumana , Landier Jordi , Gaudart Jean
Published: November 2022

Introduction: Despite the implementation of control strategies at the national scale, the malaria burden remains high in Mali, with more than 2.8 million cases reported in 2019. In this context, a new approach is needed, which accounts for the spatio-temporal variability of malaria transmission at the local scale. This study aimed to describe the spatio-temporal variability of malaria incidence and the associated meteorological and environmental factors in the health district of Kati, Mali. Methods: Daily malaria cases were collected from the consultation records of the 35 health areas of Kati’s health district, for the period 2015–2019. Data on rainfall, relative humidity, temperature, wind speed, the normalized difference vegetation index, air pressure, and land use–land cover were extracted from open-access remote sensing sources, while data on the Niger River’s height and flow were obtained from the National Department of Hydraulics. To reduce the dimension and account for collinearity, strongly correlated meteorological and environmental variables were combined into synthetic indicators (SI), using a principal component analysis. A generalized additive model was built to determine the lag and the relationship between the main SIs and malaria incidence. The transmission periods were determined using a change-point analysis. High-risk clusters (hotspots) were detected using the SatScan method and were ranked according to risk level, using a classification and regression tree analysis. Results: The peak of the malaria incidence generally occurred in October. Peak incidence decreased from 60 cases per 1000 person–weeks in 2015, to 27 cases per 1000 person–weeks in 2019. The relationship between the first SI (river flow and height, relative humidity, and rainfall) and malaria incidence was positive and almost linear. A non-linear relationship was found between the second SI (air pressure and temperature) and malaria incidence. Two transmission periods were determined per year: a low transmission period from January to July—corresponding to a persisting transmission during the dry season—and a high transmission period from July to December. The spatial distribution of malaria hotspots varied according to the transmission period. Discussion: Our study confirmed the important variability of malaria incidence and found malaria transmission to be associated with several meteorological and environmental factors in the Kati district. The persistence of malaria during the dry season and the spatio-temporal variability of malaria hotspots reinforce the need for innovative and targeted strategies.
A Pilot Study on a Nurse Rehabilitation Program: Could It Be Applied to COVID-19 Patients?
Authors: Moreira José , Fonseca Pedro , Miguel Susana
Published: November 2022

(1) Background: An aging population, pollution and an increase in life habits that are harmful to respiratory health, and more recently the COVID-19 pandemic, have led to an increase in chronic respiratory diseases. Thus, this pilot study aims to describe an intervention program on the training of respiratory patients to maintain airway permeability and preventing complications. (2) Methods: An observational, analytical, and prospective study was proposed. After the initial evaluation of each user during hospitalization, the program started with two sessions, at discharge for the second phase, and fifteen days after discharge for the third phase. Throughout the program’s implementation, the modified Medical Research Council scale and the Barthel Index were applied. (3) Results: The population studied aged between 39 and 76 years, diagnosed with pneumonia or chronic obstructive pulmonary disease, showed a significant improvement in the degrees of dyspnea and levels of functionality, as well as an adequate level of learning. (4) Conclusions: This program positively impacted the patients’ independence by reducing dyspnea and increasing functionality of the patients included in this study. The set of exercises and techniques can be replicated at home and may be fundamental in the management of respective recovery, as well as in the COVID-19 pandemic.
Technology Advancements and Employees’ Qualitative Job Insecurity in the Republic of Korea: Does Training Help? Employer-Provided vs. Self-Paid Training
Authors: Lee Hyun Jung , Probst Tahira M. , Bazzoli Andrea , Lee Sunhee
Published: November 2022

While technological advancements have proliferated in our daily lives, they also pose threats to the job security of employees. Despite these growing concerns about technology-related job insecurity, little research has been carried out on the antecedents and outcomes of tech-related job insecurity. Using a cross-sectional, nationally representative survey sample of 28,989 Korean workers drawn from the Korean Working Conditions Survey, we examined the impacts of technology advancements on employee perceptions of technology-related qualitative job insecurity (i.e., perceived technology-related threat to the continued existence of valued job features) and subsequent effects on employees’ work (i.e., work engagement, job satisfaction), health (i.e., sleep), and life (i.e., work-to-family conflict) outcomes. Furthermore, we investigated the extent to which employer-provided (versus self-funded) training buffers the adverse impacts of technology advancements and associated job insecurity. The path analysis results showed more technology changes were associated with higher job insecurity, which subsequently related to adverse outcomes. While employer-provided training helped workers to reduce the negative impacts of tech changes on job insecurity, workers who paid for their training reported more adverse outcomes in face of job insecurity. We discuss these results in light of the job demands–resources theory and practical implications to buffer the adverse impacts of technology advancements.
Tamalin Function Is Required for the Survival of Neurons and Oligodendrocytes in the CNS
Authors: Seo Yongbo , Mo Seojung , Kim Suhyun , Kim Hyun , Park Hae-Chul
Published: November 2022

Tamalin is a post-synaptic scaffolding protein that interacts with group 1 metabotropic glutamate receptors (mGluRs) and several other proteins involved in protein trafficking and cytoskeletal events, including neuronal growth and actin reorganization. It plays an important role in synaptic plasticity in vitro by controlling the ligand-dependent trafficking of group 1 mGluRs. Abnormal regulation of mGluRs in the central nervous system (CNS) is associated with glutamate-mediated neurodegenerative disorders. However, the pathological consequences of tamalin deficiency in the CNS are unclear. In this study, tamalin knockout (KO) zebrafish and mice exhibited neurodegeneration along with oligodendrocyte degeneration in the post-embryonic CNS to adulthood without any developmental defects, thus suggesting the function of tamalin is more important in the postnatal stage to adulthood than that in CNS development. Interestingly, hypomyelination was independent of axonal defects in the CNS of tamalin knockout zebrafish and mice. In addition, the loss of Arf6, a downstream signal of tamalin scaffolding protein, synergistically induced neurodegeneration in tamalin KO zebrafish even in the developing CNS. Furthermore, tamalin KO zebrafish displayed increased mGluR5 expression. Taken together, tamalin played an important role in neuronal and oligodendrocyte survival and myelination through the regulation of mGluR5 in the CNS.
Impact of Melatonin on RAW264.7 Macrophages during Mechanical Strain
Authors: Paddenberg Eva , Forneck Anne , Widbiller Matthias , Smeda Martyna , Jantsch Jonathan , Proff Peter , Kirschneck Christian , Schröder Agnes
Published: November 2022

The concentration of melatonin is elevated during the night when patients mainly wear removable orthodontic appliances. Next to periodontal ligament fibroblasts and osteoblasts, macrophages react to mechanical strain with an increased expression of inflammatory mediators. Here, we investigated the impact of melatonin on RAW264.7 macrophages exposed to tensile or compressive strain occurring during orthodontic tooth movement in the periodontal ligament. Before exposure to mechanical strain for 4 h, macrophages were pre-incubated with different melatonin concentrations for 24 h, to determine the dependence of melatonin concentration. Afterwards, we performed experiments with and without mechanical strain, the most effective melatonin concentration (25 µM), and the melatonin receptor 2 (MT2) specific antagonist 4P-PDOT. The expression of inflammatory genes and proteins was investigated by RT-qPCR, ELISAs, and immunoblot. Both tensile and compressive strain increased the expression of the investigated inflammatory factors interleukin-1-beta, interleukin-6, tumor necrosis factor alpha, and prostaglandin endoperoxide synthase-2. This effect was inhibited by the addition of melatonin. Incubation with 4P-PDOT blocked this anti-inflammatory effect of melatonin. Melatonin had an anti-inflammatory effect on macrophages exposed to mechanical strain, independent of the type of mechanical strain. As inhibition was possible with 4P-PDOT, the MT2 receptor might be involved in the regulation of the observed effects.