bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Chemotherapy Agents on Circulating Leukocyte Populations: Potential Implications for the Success of CAR-T Cell Therapies
Authors: Truong Nga T. H. , Gargett Tessa , Brown Michael P. , Ebert Lisa M.
Published: May 2021

Adoptive T-cell therapy using autologous T cells genetically modified to express cancer-specific chimeric antigen receptors (CAR) has emerged as a novel approach for cancer treatment. CAR-T cell therapy has been approved in several major jurisdictions for treating refractory or relapsed cases of B-cell precursor acute lymphoblastic leukaemia and diffuse large B-cell lymphoma. However, in solid cancer patients, several clinical studies of CAR-T cell therapy have demonstrated minimal therapeutic effects, thus encouraging interest in better integrating CAR-T cells with other treatments such as conventional cytotoxic chemotherapy. Increasing evidence shows that not only do chemotherapy drugs have tumoricidal effects, but also significantly modulate the immune system. Here, we discuss immunomodulatory effects of chemotherapy drugs on circulating leukocyte populations, including their ability to enhance cytotoxic effects and preserve the frequency of CD8+ T cells and to deplete immunosuppressive populations including regulatory T cells and myeloid-derived suppressor cells. By modulating the abundance and phenotype of leukocytes in the blood (the ‘raw material’ for CAR-T cell manufacturing), we propose that prior chemotherapy could facilitate production of the most effective CAR-T cell products. Further research is required to directly test this concept and identify strategies for the optimal integration of CAR-T cell therapies with cytotoxic chemotherapy for solid cancers.
Role of the Holoenzyme PP1-SPN in the Dephosphorylation of the RB Family of Tumor Suppressors During Cell Cycle
Authors: Verdugo-Sivianes Eva M. , Carnero Amancio
Published: May 2021

Cell cycle progression is highly regulated by modulating the phosphorylation status of the retinoblastoma protein (pRB) and the other two members of the RB family, p107 and p130. This process is controlled by a balance in the action of kinases, such as the complexes formed by cyclin-dependent kinases (CDKs) and cyclins, and phosphatases, mainly the protein phosphatase 1 (PP1). However, while the phosphorylation of the RB family has been largely studied, its dephosphorylation is less known. Phosphatases are holoenzymes formed by a catalytic subunit and a regulatory protein with substrate specificity. Recently, the PP1-Spinophilin (SPN) holoenzyme has been described as the main phosphatase responsible for the dephosphorylation of RB proteins during the G0/G1 transition and at the end of G1. Moreover, SPN has been described as a tumor suppressor dependent on PP1 in lung and breast tumors, where it promotes tumorigenesis by increasing the cancer stem cell pool. Therefore, a connection between the cell cycle and stem cell biology has also been proposed via SPN/PP1/RB proteins.
Evaluation of Serum Neurofilament Light Chain and Glial Fibrillary Acidic Protein as Screening and Monitoring Biomarkers for Brain Metastases
Authors: Kim Su-Hyun , Gwak Ho-Shin , Lee Youngjoo , Park Na-Young , Han Mira , Kim Yeseul , Kim So-Yeon , Kim Ho Jin
Published: May 2021

We evaluated the potential serum neurofilament light chain (sNfL) and serum glial fibrillary acidic protein (sGFAP) roles in diagnosing and monitoring brain metastases (BMs). We included 70 patients with newly diagnosed BMs, 71 age- and cancer type-matched patients without BMs, and 67 healthy controls (HCs). We compared sNfL and sGFAP levels among the groups using a single-molecule array immunoassay. We prospectively followed 26 patients with BMs every 2–3 months by measuring sNfL and sGFAP levels and performing magnetic resonance imaging (MRI) scans. The sNfL and the sGFAP levels were higher in patients with BMs (medians: sNfL, 63.7 µL; sGFAP, 819.5 pg/µL) than in those without BMs (sNfL, 13.3 µL; sGFAP, 154 pg/µL; p < 0.001) and HCs (sNfL, 12.5 µL; sGFAP, 135 pg/µL; p < 0.001). The sNfL and the sGFAP cutoff levels had a sensitivity and a specificity of 91%. The sGFAP cutoff level had a sensitivity of 91% and a specificity of 97%. The sNfL and the sGFAP levels were related to the BM size but not to the primary cancer type. After BM treatment, sNfL and sGFAP levels decreased with reduced BM lesions on MRI; however, they increased when BMs progressed. sNfL and sGFAP are potential biomarkers for BM screening in cancer patients.
3T MRI-Radiomic Approach to Predict for Lymph Node Status in Breast Cancer Patients
Authors: Santucci Domiziana , Faiella Eliodoro , Cordelli Ermanno , Sicilia Rosa , de Felice Carlo , Zobel Bruno Beomonte , Iannello Giulio , Soda Paolo
Published: May 2021

Background: axillary lymph node (LN) status is one of the main breast cancer prognostic factors and it is currently defined by invasive procedures. The aim of this study is to predict LN metastasis combining MRI radiomics features with primary breast tumor histological features and patients’ clinical data. Methods: 99 lesions on pre-treatment contrasted 3T-MRI (DCE). All patients had a histologically proven invasive breast cancer and defined LN status. Patients’ clinical data and tumor histological analysis were previously collected. For each tumor lesion, a semi-automatic segmentation was performed, using the second phase of DCE-MRI. Each segmentation was optimized using a convex-hull algorithm. In addition to the 14 semantics features and a feature ROI volume/convex-hull volume, 242 other quantitative features were extracted. A wrapper selection method selected the 15 most prognostic features (14 quantitative, 1 semantic), used to train the final learning model. The classifier used was the Random Forest. Results: the AUC-classifier was 0.856 (label = positive or negative). The contribution of each feature group was lower performance than the full signature. Conclusions: the combination of patient clinical, histological and radiomics features of primary breast cancer can accurately predict LN status in a non-invasive way.
Cell-Free DNA Analysis by Whole-Exome Sequencing for Hepatocellular Carcinoma: A Pilot Study in Thailand
Authors: Kunadirek Pattapon , Chuaypen Natthaya , Jenjaroenpun Piroon , Wongsurawat Thidathip , Pinjaroen Nutcha , Sirichindakul Pongserath , Nookaew Intawat , Tangkijvanich Pisit
Published: May 2021

Cell-free DNA (cfDNA) has been used as a non-invasive biomarker for detecting cancer-specific mutations. However, the mutational profile of cfDNA in Thai patients with hepatocellular carcinoma (HCC) has not been investigated. Here, we demonstrated the utility of using whole-exome sequencing (WES) of cfDNA to define the somatic mutation profiles of HCC in Thai patients. The comprehensive profile of cfDNA was determined with WES to identify variants in matched cfDNA and germline DNA from 30 HCC patients in Thailand who underwent nonoperative therapies. The level of cfDNA was higher in HCC patients compared with chronic hepatitis patients (p-value < 0.001). Single nucleotide variants were present in somatic genes in cfDNA, including in ZNF814 (27%), HRNR (20%), ZNF492 (20%), ADAMTS12 (17%), FLG (17%), OBSCN (17%), TP53 (17%), and TTN (17%). These same mutations were matched to HCC mutation data from The Cancer Genome Atlas (TCGA) and a previous Thai HCC study. The co-occurrence of HRNR and TTN mutations in cfDNA was associated with shorter overall survival in HCC patients (hazard ratio = 1.60, p-value = 0.0196). These findings indicate that the mutational profile of cfDNA accurately reflected that of HCC tissue and suggest that cfDNA could serve as a useful biomarker for diagnosis and prognosis in Thai HCC patients. In addition, we demonstrated the use of the pocket-sized sequencer of Oxford Nanopore Technology to detect copy-number variants in HCC tissues that could be applied for onsite clinical detection/monitoring of HCC.
Psychological Resilience and Health-Related Quality of Life in 418 Swedish Women with Primary Breast Cancer: Results from a Prospective Longitudinal Study
Authors: Mohlin Åsa , Bendahl Pär-Ola , Hegardt Cecilia , Richter Corinna , Hallberg Ingalill Rahm , Rydén Lisa
Published: May 2021

Psychological resilience is considered a major protective psychological mechanism that enables a person to successfully handle significant adversities, e.g., a cancer diagnosis. Higher levels of resilience have been associated with higher levels of health-related quality of life (HRQoL) in breast cancer (BC) patients, but research examining the longitudinal process of resilience is limited. The aim of this population-based longitudinal study was to investigate resilience and HRQoL from diagnosis to one year later in 418 Swedish women with primary BC. Resilience was measured with the Connor–Davidson Resilience Scale 25, and HRQoL was measured with the Short Form Health Survey. The participants responded to questions regarding demographic and study-specific variables. Clinicopathological variables were collected from the Swedish National Quality Register for Breast Cancer. The mean score for resilience was 70.6 (standard deviation, SD = 13.0) at diagnosis and 68.9 (SD = 14.0) one year later, p < 0.001. The level of trust in the treatment and financial situation demonstrated the greatest association with the change in resilience levels. No oncological treatment modality was associated with a change in resilience levels. HRQoL decreased over time in the cohort. Resilience was positively associated with HRQoL at one year post diagnosis, which demonstrates that resilience is an important factor in maintaining HRQoL.
The Ubiquitin Proteasome System in Genome Stability and Cancer
Authors: Morgan Jonathan J. , Crawford Lisa J.
Published: May 2021

Faithful DNA replication during cellular division is essential to maintain genome stability and cells have developed a sophisticated network of regulatory systems to ensure its integrity. Disruption of these control mechanisms can lead to loss of genomic stability, a key hallmark of cancer. Ubiquitination is one of the most abundant regulatory post-translational modifications and plays a pivotal role in controlling replication progression, repair of DNA and genome stability. Dysregulation of the ubiquitin proteasome system (UPS) can contribute to the initiation and progression of neoplastic transformation. In this review we provide an overview of the UPS and summarize its involvement in replication and replicative stress, along with DNA damage repair. Finally, we discuss how the UPS presents as an emerging source for novel therapeutic interventions aimed at targeting genomic instability, which could be utilized in the treatment and management of cancer.
Metronomic Chemotherapy
Authors: Cazzaniga Marina Elena , Cordani Nicoletta , Capici Serena , Cogliati Viola , Riva Francesca , Cerrito Maria Grazia
Published: May 2021

Metronomic chemotherapy treatment (mCHT) refers to the chronic administration of low doses chemotherapy that can sustain prolonged, and active plasma levels of drugs, producing favorable tolerability and it is a new promising therapeutic approach in solid and in hematologic tumors. mCHT has not only a direct effect on tumor cells, but also an action on cell microenvironment, by inhibiting tumor angiogenesis, or promoting immune response and for these reasons can be considered a multi-target therapy itself. Here we review the state of the art of mCHT use in some classical tumour types, such as breast and no small cell lung cancer (NSCLC), see what is new regarding most recent data in different cancer types, such as glioblastoma (GBL) and acute myeloid leukemia (AML), and new drugs with potential metronomic administration. Finally, a look at the strategic use of mCHT in the context of health emergencies, or in low –and middle-income countries (LMICs), where access to adequate healthcare is often not easy, is mandatory, as we always need to bear in in mind that equity in care must be a compulsory part of our medical work and research.
Synthesis of Highly Porous Cu2O Catalysts for Efficient Ozone Decomposition
Authors: Jiang Yishan , Chen Juna , Zhao Xin , Ma Guojun
Published: May 2021

At present, it is urgent to synthesize highly active ozone decomposition catalysts to cope with the ever-increasing ozone concentration in the atmosphere. In this study, a highly porous Cu2O catalyst was prepared by using combined surfactants of triblock copolymer P123 and n-butanol through a simple solution reduction method by ascorbic acid. Transmittance electron microscopy, X-ray diffraction, and N2 adsorption–desorption characterizations verify the highly porous structure with a relatively high surface area of 79.5 m2·g−1 and a small crystallite size of 2.7 nm. The highly porous Cu2O shows 90% ozone conversion activity in harsh conditions, such as a high space velocity of 980,000 cm3·g−1·h−1, or a high relative humidity of 90% etc., which is not only attributable to the high surface area but also to the high concentration of surface oxygen vacancy. The results show the promising prospect of the easily synthesized, highly porous Cu2O for effective ozone decomposition applications.
Heterogeneous Catalysts for Petrochemical Synthesis and Oil Refining
Authors: Glotov Aleksandr , Karakhanov Eduard
Published: May 2021

In modern industry, more than 90% of processes are catalytic [...]
Chronic Alcohol Exposure of Cells Using Controlled Alcohol-Releasing Capillaries
Authors: Kim Wanil , Jeong Hye-Seon , Kim Sang-Chan , Choi Chang-Hyung , Lee Kyung-Ha
Published: May 2021

Alcohol is one of the main causes of liver diseases such as fatty liver, alcoholic hepatitis, and chronic hepatitis with liver fibrosis or cirrhosis. To reproduce the conditions of alcohol-induced liver diseases and to identify the disease-causing mechanisms at the cellular level, several methods have been used to expose the cells to ethanol. As ethanol evaporates easily, it is difficult to mimic chronic alcohol exposure conditions at the cellular level. In this study, we developed a glass capillary system containing ethanol, which could steadily release ethanol from the polyethylene tubing and hydrogel portion at both sides of the capillary. The ethanol-containing capillary could release ethanol in the cell culture medium for up to 144 h, and the concentration of ethanol in the cell culture medium could be adjusted by controlling the number of capillaries. A long-term exposure to ethanol by the capillary system led to an increased toxicity of cells and altered the cellular physiologies, such as increasing the lipid accumulation and hepatic transaminase release in cells, as compared to the traditional direct ethanol addition method. Ethanol capillaries showed different gene expression patterns of lipid accumulation- or chronic alcoholism-related genes. Our results suggest that our ethanol-containing capillary system can be used as a valuable tool for studying the mechanism of chronic alcohol-mediated hepatic diseases at the cellular level.
Assessment of Computational Tools for Predicting Supramolecular Synthons
Authors: Sandhu Bhupinder , McLean Ann , Sinha Abhijeet S. , Desper John , Aakerӧy Christer B.
Published: May 2021

The ability to predict the most likely supramolecular synthons in a crystalline solid is a valuable starting point for subsequently predicting the full crystal structure of a molecule with multiple competing molecular recognition sites. Energy and informatics-based prediction models based on molecular electrostatic potentials (MEPs), hydrogen-bond energies (HBE), hydrogen-bond propensity (HBP), and hydrogen-bond coordination (HBC) were applied to the crystal structures of twelve pyrazole-based molecules. HBE, the most successful method, correctly predicted 100% of the experimentally observed primary intermolecular-interactions, followed by HBP (87.5%), and HBC = MEPs (62.5%). A further HBC analysis suggested a risk of synthon crossover and synthon polymorphism in molecules with multiple binding sites. These easy-to-use models (based on just 2-D chemical structure) can offer a valuable risk assessment of potential formulation challenges.
Attitude Changes of Stakeholders towards Climate Change Adaptation Policies in Agricultural Sector by Online Deliberation
Authors: Baba Kenshi , Amanuma Eri , Kosugi Motoko
Published: May 2021

This study set up online virtual communities consisting of farmers and stakeholders involved in agriculture and nonfarmers living in rural areas interested in agricultural production. We conducted a deliberation within the communities for 14 days on identifying important climate change adaptation policies for 30 years later under climate change impacts with the relevant knowledge from experts. During the deliberation, after self-introduction took place including the realization of climate change impacts, the participants were provided with the expert knowledge on impacts of climate change, adaptation policies in agricultural sector and so on, then the following discussions covered issues such as the distribution of agricultural produce, insufficient successors, and support for farmers, such as impacts on crops during disasters concerning future scenarios. Attitude changes before and after deliberation were observed in terms of the pros and cons of climate change adaptation policies in agriculture and rural areas, but statistically significant differences were not observed. On the other hand, a statistically significant change was observed in some determinants of the pros and cons, such as the perceived effectiveness and goal intention. This structural change results from that the participants became aware of a different perspective through deliberation. Thus, the online deliberation process was effective to some extent in increasing knowledge and promoting deeper understanding among participants during inquiry and reasoning was deepened in the process as they listened to the opinions of others in a different position with a different idea as well as read and search for scientific findings and information provided by experts.
SARS-CoV-2-Associated Obliterative Arteritis Causing Massive Testicular Infarction
Authors: Parrón Dámaso , Gartzia Ane , Iturregui Ane M. , Imaz Igone , Manini Claudia , García-Olaverri Jorge , López José I.
Published: May 2021

A 26-year-old man with symptomatic SARS-CoV-2 infection developed a sudden-onset acute testicular pain. The echo-doppler images showed massive testicular infarction, so orchiectomy was performed. On gross examination, the surgical specimen showed complete testicular necrosis and diffuse thickening of the testicular coverings. Under the microscope, a severe obliterative arteritis was evidenced. SARS-CoV-2 spike antibody was detected by immunohistochemistry in the arterial endothelium. Electron microscopy displayed intracytoplasmic spiky viral particles in endothelial cells. The patient was treated with corticoids and was asymptomatic at last contact.
Surface and Interface Investigation of a 15th Century Wall Painting Using Multispectral Imaging and Pulse-Compression Infrared Thermography
Authors: Ricci Marco , Laureti Stefano , Malekmohammadi Hamed , Sfarra Stefano , Lanteri Luca , Colantonio Claudia , Calabrò Giuseppe , Pelosi Claudia
Published: May 2021

Before starting the restoration of an artwork, good practice involves the evaluation of the item healthiness possibly carried out through non-invasive techniques. In the specific case of painting inspection, hyper- and multi- spectral techniques are commonly used to analyze the outer layers (varnish, pictorial, and drawing), while X-ray, tomography, and many others can be employed to investigate its inner structure. Although highly desirable, a single technique providing all the information about a painting is still not available. Thus, it is of great interest to define the analysis’ protocols that could optimally exploit the complementarities of a minimal set of techniques. To this aim, the present paper shows the combined use of the hypercolorimetric multispectral imaging and pulse-compression thermography on a 15th century wall painting attributed to the Italian artist Antonio del Massaro, also known as Pastura, representing the Madonna with the Child and the Saints Jerome and Francis. The capabilities and the complementarities of the two techniques, whose information can also be fused through post-processing, are illustrated in detail in this paper. In addition, a false-colour imaging approach is proposed to improve the readability and analysis of the thermography results.
Effect of Carrier Gas Flow Field on Chemical Vapor Deposition of 2D MoS2 Crystal
Authors: Bai Minyu , Wen Shuai , Zhao Jijie , Du Yuxuan , Xie Fei , Liu Huan
Published: May 2021

The carrier gas flow field plays a vital role in the chemical vapor deposition (CVD) process of two dimensional (2D) MoS2 crystal, which was studied by simulations and experiments. Different carrier gas flow fields were studied by utilizing three types of precursor carrier which affected the local gas flow field significantly. The experiment results showed that the appropriate precursor vapor concentration could be achieved by local carrier gas flow field conditioning, resulting in single 2D MoS2 crystals of a large size and a high coating rate of 2D MoS2 crystal on the target substrate surface. The carrier gas flow also contributed to the growth of the 2D MoS2 crystal when it flew towards the target surface. The size of deposited single 2D MoS2 crystal reached tens of micrometers and a few layers of 2D MoS2 crystal were characterized and confirmed.
Effect of Synthesis Method of Nickel–Samarium-Doped Ceria Anode on Distribution of Triple-Phase Boundary and Electrochemical Performance
Authors: Shaikh Abdul Muhammed Ali , Yahaya Ahmad Zubair , Anwar Mustafa , Soo Mun Teng , Muchtar Andanastuti , Kovrugin Vadim M.
Published: May 2021

Two-dimensional (2D) electron back scattered diffraction (EBSD) is a powerful tool for microstructural characterization of crystalline materials. EBSD enables visualization and quantification of the effect of synthesis methods on the microstructure of individual grains, thus correlating the microstructure to mechanical and electrical efficiency. Therefore, this work was designed to investigate the microstructural changes that take place in the Ni-SDC cermet anode under different synthesis methods, such as the glycine–nitrate process (GNP) and ball-milling. EBSD results revealed that different grain size and distribution of Ni and SDC phases considerably influenced the performance of the Ni–SDC cermet anodes. The performance of the Ni–SDC cermet anode from GNP was considerably higher than that of Ni-SDC from ball-milling, which is attributed to the triple-phase boundary (TPB) density and phase connectivity. Due to the poor connectivity between the Ni and SDC phases and the development of large Ni and SDC clusters, the Ni-SDC cermet anode formed by ball milling had a lower mechanical and electrical conductivity. Moreover, the Ni–SDC cermet anode sample obtained via GNP possessed sufficient porosity and did not require a pore former. The length and distribution of the active TPB associated with phase connectivity are crucial factors in optimizing the performance of Ni-SDC cermet anode materials. The single cell based on the Ni–SDC composite anode prepared through GNP exhibited a maximum power density of 227 mW/cm2 and 121 mW/cm2 at 800 °C in H2 and CH4, respectively.
Electrically Tunable Terahertz Focusing Modulator Enabled by Liquid Crystal Integrated Dielectric Metasurface
Authors: Shen Yanchun , Shen Zhixiong , Wang Yuye , Xu Degang , Hu Wei
Published: May 2021

Active lenses with focal tunable properties are highly desired in the modern imaging systems from the visible to the microwaves. In this paper, we demonstrate a terahertz (THz) lens with electrically switchable focal length. It is composed of a large-birefringence liquid crystal (LC) layer infiltrating a dielectric metasurface. When the birefringence of LC is tuned with an external bias, the phase shift of a single meta-unit will change. With parameter sweep using the finite-different time-domain (FDTD) simulation method, meta-units with varying geometries are optimized to achieve a focal length switchable metalens. The numerical results show that the focal length can be switched between 8.3 mm and 10.5 mm at bias OFF and ON states, respectively, which is consistent with the design. A feasible fabrication procedure of the lens is further discussed. Such a device can be designed beyond the THz band to the visible or the microwaves, and may be widely applied in integrated imaging systems.
Cancer Screening Interventions in Indigenous Populations: A Rapid Review
Authors: Bryant Janell , Patterson Kara , Vaska Marcus , Chiang Bonnie , Letendre Angeline , Bill Lea , Yang Huiming , Kopciuk Karen
Published: May 2021

Cancer screening is an important component of a cancer control strategy. Indigenous people in Canada have higher incidence rates for many types of cancer, including those that can be detected early or prevented through organized screening programs. Increased participation and retention in cancer screening is critical to improved population health outcomes amongst Indigenous people. This rapid review evaluates cancer screening interventions published in the last six years. Included studies demonstrated increased participation in breast, colorectal, or cervical cancer screening programs in Indigenous populations or showed promise of increased participation based on the factors that influence people’s screening practices, such as knowledge, attitude, or intent to screen. The Preferred Reporting Items for Systematic Reviews guided the search strategy. The review identified 85 articles with 12 meeting the specified criteria: seven studies reported an increase in cancer screening participation and five studies reported improved knowledge, attitude, or intent to screen. The use of multiple culturally appropriate strategies in co-designed studies were the most effective. This review will be used to inform First Nations (FN) populations and Screening Programs in Alberta of potential strategies to address disparities identified through a recent data analysis comparing cancer screening and outcomes between FN and non-FN people.
Current Challenges of Cardiac Amyloidosis Awareness among Romanian Cardiologists
Authors: Adam Robert , Neculae Gabriela , Stan Claudiu , Jurcut Ruxandra
Published: May 2021

Cardiac amyloidosis (CA) is a restrictive cardiomyopathy characterized by deposition of amyloid in the myocardium and recent studies revealed it is more frequently seen than we thought. Advances in diagnosis and treatment have been made over the last few years that make it desirable to diagnose CA without delay, and that may require extra education. An online survey was conducted among cardiologists from Romania, representing the first assessment of the knowledge of CA among them, with 195 cardiologists answering the questionnaire. There was a wide variation in their knowledge regarding CA. Our participants had limited experience with CA and reported a significant delay between first cardiac symptoms and diagnosis. We address the gaps in knowledge that were identified as educational opportunities in the main identified areas: prevalence and treatment of wild type transthyretin amyloidosis (ATTRwt), prevalence of variant transthyretin amyloidosis (ATTRv) in Romania, diagnosis of CA, the delay in CA diagnosis and available treatment options. Awareness among cardiologists is the most important challenge in diagnosing CA. Romanian cardiologists are partially aware of this topic, but there are still gaps in their knowledge. Educational programs can improve screening of patients with a high suspicion for this progressive condition the prognosis of which has been dramatically changed by the new treatment options.
Cancer-Associated Thrombosis: A New Light on an Old Story
Authors: Shah Sidrah , Karathanasi Afroditi , Revythis Antonios , Ioannidou Evangelia , Boussios Stergios
Published: May 2021

Cancer-associated thrombosis (CAT) is a rising and significant phenomenon, becoming the second leading cause of death in cancer patients. Pathophysiology of CAT differs from thrombosis in the non-cancer population. There are additional risk factors for thrombosis specific to cancer including cancer type, histology, and treatment, such as chemotherapy. Recently developed scoring systems use these risk factors to stratify the degree of risk and encourage thromboprophylaxis in intermediate- to high-risk patients. Anticoagulation is safely used for prophylaxis and treatment of CAT. Both of these have largely been with low-molecular-weight heparin (LMWH), rather than the vitamin K antagonist (VKA); however, there has been increasing evidence for direct oral anticoagulant (DOAC) use. Consequently, international guidelines have also adapted to recommend the role of DOACs in CAT. Using DOACs is a turning point for CAT, but further research is warranted for their long-term risk profile. This review will discuss mechanisms, risk factors, prophylaxis and management of CAT, including both LMWH and DOACs. There will also be a comparison of current international guidelines and how they reflect the growing evidence base.
Alignment of Qx100/Qx200 Droplet Digital (Bio-Rad) and QuantStudio 3D (Thermofisher) Digital PCR for Quantification of BCR-ABL1 in Ph+ Chronic Myeloid Leukemia
Authors: Fava Carmen , Bernardi Simona , Gottardi Enrico Marco , Lorenzatti Roberta , Galeotti Laura , Ceccherini Francesco , Cordoni Francesco , Daraio Filomena , Giugliano Emilia , Jovanovski Aleksandar , Petiti Jessica , Varotto Marta , Barberio Davide , Rege-Cambrin Giovanna , Berchialla Paola , Sciannameo Veronica , Malagola Michele , Saglio Giuseppe , Russo Domenico
Published: May 2021

In recent years, the digital polymerase chain reaction has received increasing interest as it has emerged as a tool to provide more sensitive and accurate detection of minimal residual disease. In order to start the process of data alignment, we assessed the consistency of the BCR-ABL1 quantification results of the analysis of 16 RNA samples at different levels of disease. The results were obtained by two different laboratories that relied on The Qx100/Qx200 Droplet Digital PCR System (Bio-Rad) and Quant Studio 3D dPCR System (Thermofisher) platforms. We assessed the compatibility between the estimated values by linear regression, Bland–Altman bias-plot, and Mann–Whitney nonparametric test. The results confirmed the compatibility of the measures, allowing us tocompute an ‘alignment factor’ (AF), equal to 1.41, which was further validated by a different series of experiments. We conclude that the performed measurements by the two laboratories are comparable, and also equalized through the introduction of an alignment factor.
Electrophysiological Properties from Computations at a Single Voltage: Testing Theory with Stochastic Simulations
Authors: Wilson Michael A. , Pohorille Andrew
Published: May 2021

We use stochastic simulations to investigate the performance of two recently developed methods for calculating the free energy profiles of ion channels and their electrophysiological properties, such as current–voltage dependence and reversal potential, from molecular dynamics simulations at a single applied voltage. These methods require neither knowledge of the diffusivity nor simulations at multiple voltages, which greatly reduces the computational effort required to probe the electrophysiological properties of ion channels. They can be used to determine the free energy profiles from either forward or backward one-sided properties of ions in the channel, such as ion fluxes, density profiles, committor probabilities, or from their two-sided combination. By generating large sets of stochastic trajectories, which are individually designed to mimic the molecular dynamics crossing statistics of models of channels of trichotoxin, p7 from hepatitis C and a bacterial homolog of the pentameric ligand-gated ion channel, GLIC, we find that the free energy profiles obtained from stochastic simulations corresponding to molecular dynamics simulations of even a modest length are burdened with statistical errors of only 0.3 kcal/mol. Even with many crossing events, applying two-sided formulas substantially reduces statistical errors compared to one-sided formulas. With a properly chosen reference voltage, the current–voltage curves can be reproduced with good accuracy from simulations at a single voltage in a range extending for over 200 mV. If possible, the reference voltages should be chosen not simply to drive a large current in one direction, but to observe crossing events in both directions.
Asymptotic and Finite Sample Properties for Multivariate Rotated GARCH Models
Authors: Asai Manabu , Chang Chia-Lin , McAleer Michael , Pauwels Laurent
Published: May 2021

This paper derives the statistical properties of a two-step approach to estimating multivariate rotated GARCH-BEKK (RBEKK) models. From the definition of RBEKK, the unconditional covariance matrix is estimated in the first step to rotate the observed variables in order to have the identity matrix for its sample covariance matrix. In the second step, the remaining parameters are estimated by maximizing the quasi-log-likelihood function. For this two-step quasi-maximum likelihood (2sQML) estimator, this paper shows consistency and asymptotic normality under weak conditions. While second-order moments are needed for the consistency of the estimated unconditional covariance matrix, the existence of the finite sixth-order moments is required for the convergence of the second-order derivatives of the quasi-log-likelihood function. This paper also shows the relationship between the asymptotic distributions of the 2sQML estimator for the RBEKK model and variance targeting quasi-maximum likelihood estimator for the VT-BEKK model. Monte Carlo experiments show that the bias of the 2sQML estimator is negligible and that the appropriateness of the diagonal specification depends on the closeness to either the diagonal BEKK or the diagonal RBEKK models. An empirical analysis of the returns of stocks listed on the Dow Jones Industrial Average indicates that the choice of the diagonal BEKK or diagonal RBEKK models changes over time, but most of the differences between the two forecasts are negligible.
India’s Road to Independence in Manufacturing Active Pharmaceutical Ingredients: Focus on Essential Medicines
Authors: Cherian Jerin Jose , Rahi Manju , Singh Shubhra , Reddy Sanapareddy Eswara , Gupta Yogendra Kumar , Katoch Vishwa Mohan , Kumar Vijay , Selvaraj Sakthivel , Das Payal , Gangakhedkar Raman Raghunathrao , Dinda Amit Kumar , Sarkar Swarup , Vaghela Puroshottambhai Devshibhai , Bhargava Balram
Published: May 2021

Background: Active Pharmaceutical Ingredient (API) manufacturing is an important segment of the Indian pharma industry. India ranks third in terms of volume of medicines produced, and is a major global supplier of generic medicines. However, India depends heavily on the import of several raw materials used to produce some of these medicines. Main Body: This paper highlights how the situation emerged, as well as the various steps taken by the government to reduce import dependency and promote domestic manufacturing of APIs. Through various consultations with government officials, policy analysts and academicians, and interactions with key industry experts, the paper also highlights the public health perspectives of such dependence, especially regarding the secure access to essential medicines. India needs a conducive ecosystem to capitalize on the full potential of its API manufacturing capabilities, which may require some short-term and some mid/long-term interventions. Conclusions: It concludes by providing certain short-, medium- and long-term recommendations in the policy, fiscal and technology domains, to promote API independence. It also summarizes the various steps taken by the government in moving towards domestic manufacturing of APIs.
Polymer Additive Assisted Fabrication of Compact and Ultra-Smooth Perovskite Thin Films with Fast Lamp Annealing
Authors: Shaik Shoieb , Zhou Ziyou , Ouyang Zhongliang , Han Rebecca , Li Dawen
Published: May 2021

Perovskite solar cells (PVSC) have drawn increasing attention due to their high photovoltaic performance and low-cost fabrication with solution processability. A variety of methods have been developed to make uniform and dense perovskite thin films, which play a critical role on device performance. Herein, we demonstrate a polymer additive assisted approach with Polyamidoamine (PAMAM) dendrimers to facilitate the growth of uniform, dense, and ultra-smooth perovskite thin films. Furthermore, a lamp annealing approach has been developed to rapidly anneal perovskite films using an incandescent lamp, resulting in comparable or even better device performance compared to the control hotplate annealing. The facile polymer additive assisted method and the rapid lamp annealing technique offer a clue for the large-scale fabrication of efficient PVSCs.
Exploring the Research Regarding Energy–Economic Growth Relationship
Authors: Cicea Claudiu , Ciocoiu Carmen Nadia , Marinescu Corina
Published: May 2021

The purpose of this study is to analyze the evolution of the scientific research regarding the relationship between energy and economic growth, in order to reveal preferred topics and less approached themes. We conducted an occurrence and cluster analysis, followed by a correspondence analysis using articles published between 1979 and 2019 in journals indexed in the Web of Science. The analysis was split into three periods taking into account the major economic and energetic milestones. The analysis focused on distribution of the topics studied both by years and by journals. The research revealed some major trends: there has been an explosive increase in studies based on Asian countries over the three periods as concerns for sustainable development intensified, and environmental issues were associated with research on the relationship between energy and economic growth. Even if the journals cover different scientific areas, during the last 10 years they contain articles with very similar topics.
Constraints of Parametrically Defined Guide Vanes for a High-Head Francis Turbine
Authors: Stojkovski Filip , Lazarevikj Marija , Markov Zoran , Iliev Igor , Dahlhaug Ole Gunnar
Published: May 2021

This paper is focused on the guide vane cascade as one of the most crucial stationary sub-systems of the hydraulic turbine, which needs to provide efficient inflow hydraulic conditions to the runner. The guide vanes direct the flow from the spiral casing and the stay vanes towards the runner, regulating the desired discharge. A parametric design tool with normalized geometrical constraints was created in MATLAB, suitable for generating guide vane cascade geometries for Francis turbines. The goal is to determine the limits of these constraints, which will lead to future faster prediction of initial guide vane configurations in the turbine optimal operating region. Several geometries are developed using preliminary design data of the turbine and are investigated using CFD simulations close to the best efficiency point (BEP) of the turbine. This research is part of the Horizon-2020—HydroFlex project led by the Norwegian University of Science and Technology (NTNU), focusing on the development of a flexible hydropower generation.
Optimisation of Operation of Adsorption Chiller with Desalination Function
Authors: Sztekler Karol
Published: May 2021

The demand for electricity is growing rapidly along with economic development and increasing population. At present, its production is mainly based on non-renewable sources, which has negative impacts on the environment and contributes to global warming. A large proportion of the produced electricity is consumed by refrigeration equipment. Climate change and the progress of civilisation are additionally increasing the demand for cooling, with increasing electricity consumption as a consequence. One of the options for obtaining eco-friendly cooling is the use of adsorption chillers. These devices are powered by low-temperature heat and their operation only requires a small amount of electrical energy. The source of low-temperature heat can be, e.g., waste heat generated in many industrial processes. Its use allows one to increase energy efficiency and achieve additional financial benefits. However, adsorption chillers are characterised by low coefficients of performance. This paper presents possibilities to improve their performance. It also presents the results of tests carried out on a three-bed adsorption chiller with desalination function. The aim of the investigation was to determine the effect of the cycle time on the coefficient of performance (COP) and specific cooling power (SCP). The working pair was silica gel and water. The results confirmed the effect of the duration of adsorption and desorption on the COP and SCP of the adsorption chiller. Increasing the duration of the cycle led to an increase in the COP.
Experimental Study on Reservoir Physical Properties and Formation Blockage Risk in Geothermal Water Reinjection in Xining Basin: Taking Well DR2018 as an Example
Authors: Zhao Zhen , Qin Guangxiong , Luo Yinfei , Geng Songhe , Yang Linchao , Wen Ronghua , Chao Jiahao , Zhang Liang
Published: May 2021

The Xining Basin in Qinghai Province, China, is rich in mid–low temperature geothermal resources, but the reinjection of geothermal water has not yet started. In this paper, the physical properties of rocks sampled from the newly drilled geothermal well DR2018 were analyzed, and a series of core flooding experiments was conducted to assess the formation blockage risk during water reinjection. The experimental results show that the geothermal reservoir has a low porosity of 1.64–18.68% and a low permeability of 0.04–7.23 md. The rocks are weakly consolidated, and the movable clay and sand particles account for 0.18–23.42 wt %, which results in a significant rate and salinity sensitivity. Even at low water flow velocity of 0.31–1.64 cm/min, the core permeability will drop by 35–53% after 25-144PV injection. An obvious fluctuation and decline in core permeability can also be observed as the injected water salinity decreases. The blockage risk induced by the invasion of low-content scaling and suspended particles in injected water can be covered up by the migration of movable particles in cores. The particle migration and blockage in the near-wellbore formation will be the main reason to cause the decline in the well’s geothermal reinjection capacity.
Implementation of Distributed Autonomous Control Based Battery Energy Storage System for Frequency Regulation
Authors: Kim Hyung-Seung , Hong Junho , Choi In-Sun
Published: May 2021

It has been mandated that 5% of the generation capacity of conventional fossil fuel power plants shall be used exclusively for frequency regulation (FR) purposes in South Korea. However, the rotational speed of generators cannot be controlled quickly, and thus the variation in the power generation for FR takes some time. Even during this short period of time, frequency fluctuations may occur, and the frequency may be out of range of its reference value. In order to overcome the limitations of the existing FR method, 374 MW (103 MWh) battery energy storage systems (BESSs) for FR have been installed and are in operation at 13 sites in South Korea. When designing the capacity of BESS for FR, three key factors, i.e., the deployment time, duration of delivery, and end of delivery, are considered. When these times can be reduced, the required capacity for BESS installation can be decreased, achieving the same operational effects with minimal investment in the facilities. However, because a BESS for FR (FR BESS) needs to be installed under a large capacity, providing a single output, a centralized control method is employed. The centralized control method has the advantage of being able to view and check the entire system at once, although in the case of FR BESS, a novel system design that can optimize the above three factors through a faster and more accurate control is required. Therefore, this paper proposes the implementation of a distributed autonomous control-based BESS for frequency regulation. For the proposed FR BESS, the central control system is responsible for the determination of external factors, e.g., power generation/demand forecasting; and the system is designed such that the optimal control method of renewable energy sources and BESS according to real-time frequency variations during practical operation is determined and operated using a distributed autonomous control method. Furthermore, this study was verified through the simulation that the proposed distributed autonomous control method conducts FR faster than an FR BESS with conventional centralized control, leading to an increase in the FR success rate, and a decrease in the deployment time required (e.g., 200 ms).
Effects of Oxygen and Steam Equivalence Ratios on Updraft Gasification of Biomass
Authors: Cerone Nadia , Zimbardi Francesco
Published: May 2021

Several experimental datasets available on the gasification of different lignocellulosic feedstocks were used to correlate the flow of gasifying agents with the performance of updraft gasification in an autothermic 200 kWth pilot plant. The feedstocks used included eucalyptus wood chips, torrefied eucalyptus and spruce chips, lignin rich residues from biorefined straw and reed, shells of almond and hazelnut, which were gasified in flows of air, air and steam, oxygen, oxygen and steam. Thermal profiles inside the gasifier and gas quality in terms of incondensable gas and tar content were recorded and used to calculate the energy efficiency of converting solid feedstock into gaseous and liquid carriers. Common behaviors and parametric functionalities were identified to better understand the process and the most efficient tools to achieve the desired products. In analyzing data, the ratio steam to biomass was reported in terms of the equivalence ratio, ER(H2O) i.e., the fraction of the stoichiometric quantity required to convert the feedstock into H2 and CO2. The use of steam was useful to stabilize the process and to tune the H2/CO ratio in the syngas which reached the value of 2.08 in the case of oxy-steam gasification of lignin rich residues at ER(H2O) of 0.25. Larger use of steam depressed the process by lowering the average temperature of the bed, which instead increased steadily with ER(O2). The production of tar depends on the biomass type and a substantial reduction can be achieved with the torrefaction pretreatment. The same effect was observed increasing the residence time of the syngas in the reactor, typically achieved using oxygen instead of air as main gasification flow or reducing the ER(H2O). Oxy-steam gasification of torrefied wood led to the best results in terms of cold gas efficiency and low heating value when carried out in the ranger 0.23–0.27 of both the ERs.
An IoT-Based Traceability Platform for Wind Turbines
Authors: An Jinjing , Chen Guoping , Zou Zhuo , Sun Yaojie , Liu Ran , Zheng Lirong
Published: May 2021

In recent years, the application of traceability systems in the food and drug industry has developed rapidly, but it is rarely used for wind turbines. From the aspects of low information transparency and information islands in the supply chain process for wind turbines, a reliable traceability system is essential. However, the existing traceability systems are not suitable to be directly applied to wind turbines. Consequently, according to the characteristics of the wind power industry, a semi-centralized traceability architecture based on Internet of Things technology was proposed. Furthermore, a traceability platform was constructed by analyzing the information collected in each stage related to various user needs of wind turbines, and various applications, including manufacturing management and spare parts management, were developed. Compared with the existing systems, the proposed platform was wind-turbine-oriented, effectively improved traceability efficiency and enterprises’ information security, and extended the length of the traceability chain by integrating the after-sales information. The traceability of key components of wind turbines during their life cycle provides a useful reference for further improving the parts quality management system of the wind power industry.