bluuebunny/crossref_metadata_2025 huggingface dataset:
Sistema de simulación de realidad virtual para iniciar tempranamente en los procedimientos quirúrgicos en la carrera de Medicina Humana en la Clínica Avansalud en el 2019
Authors:   , Espinoza Gutierrez Hegel
Published: March 2023

El proyecto de investigación tiene la finalidad de desarrollar un software de Realidad virtual que beneficie a la plana estudiantil de la carrera de Medicina humana con entrenamientos de diferentes casos en procedimientos quirúrgicos donde el estudiante podrá acceder a un ambiente virtual y realizar sus prácticas con la idea principal de que el alumno desarrolle su potencial en los primeros años de la carrera facilitando sus intervenciones en los casos de la vida real. La problemática inicia cuando la gran mayoría de alumnos que rotan por clínicas y hospitales llegan sin conocimientos prácticos o cuando son puestos a casos su solución no satisface los requerimientos mínimos que debería tener un alumno de medicina humana promedio haciendo referencia a su nivel de instrucción. Sin embargo, la falta de práctica no significa que el alumno no conozca la teoría, sino que simplemente no tiene un ambiente en donde aplicarlo. Por ello al optar por un desarrollo de realidad virtual se busca que la gran mayoría de los estudiantes tengan acceso a dicha herramienta, para ello se piensa implementar un sistema de realidad virtual al alcance económico de ellos. De tal forma que los alumnos puedan practicar desde su dispositivo móvil Android utilizando periféricos costo- eficientes que permitan la fluidez de las interacciones dentro del ambiente virtual. En los periféricos podemos encontrar las gafas producidas por Samsung “Gear VR” o en su defecto algún otro casco que el alumno pueda adquirir y sea compatible con su dispositivo móvil. Lo que si se recomienda es la compra del dispositivo “Leap Motion” que permite emular con sensores de proximidad los movimientos de las manos ya que para cualquier cirujano el éxito de un procedimiento es el buen uso de sus elementos.
Plan de negocios para la venta de servicios domésticos en Lima Moderna
Authors:   , Angoma Flores Rocio Socorro , Carbonell Navarrete Piero Alexis , Muñoz Lara Gaby , Navarrete Urquizo Karla Gianella , Vera Tudela Tenorio Cristhian Vicente
Published: March 2023

Hace más de 1 año el mundo ha cambiado y Perú no fue la excepción. A causa del Covid-19, la rutina de adquisición de los consumidores cambió en el trabajo, estudio, diversión, etc. Las personas pasan mucho más tiempo en su hogar realizando un sinfín de actividades; ante este nuevo estilo de vida, es normal que las personas realicen compras por internet, para recibir los productos en casa. Hoy en día, la mayor parte del tiempo se pasa en casa y para sacar mayor provecho a su hogar los consumidores han repotenciado sus espacios para poder trabajar de manera más cómoda y que sus hijos puedan estudiar mejor. A su vez los consumidores buscan recomendaciones sobre técnicos para el hogar, para mejorar sus casas o para solucionar un desperfecto de emergencia. Por todo lo antes mencionado nace nuestra propuesta de negocio, ante la necesidad de los consumidores de encontrar personal calificado, de confianza y disponible las 24 horas del día ante una eventual emergencia. nuestra propuesta de negocio es recopilar todas estas necesidades para poder cubrir estas demandas. TDateo Soluciones es una empresa de servicios para el hogar mediante una web, donde el cliente pueda encontrar alguna alternativa de solución a su necesidad, ya sea gasfitería, electricista, cerrajero, pintor, carpintero, técnico en computación, albañil, etc. Es importante encontrar en un solo lugar el servicio que necesita, contando con personal calificado, con altos estándares de calidad, siendo puntuales y marcando una diferencia de disponibilidad las 24 hora del día. hoy en día no se encuentra en el mercado una empresa con los diversos servicios que ofrecemos en un solo lugar.
Aspectos fisiológicos, epidemiológicos y clasificación de las garrapatas (Familia Ixodidae) que afectan a animales de compañía
Authors:   , Jiménez Segura Julio André
Published: March 2023

Las garrapatas de la familia Ixodidae, denominadas garrapatas duras, son vectores de enfermedades con importancia en el área de salud pública; por lo que se suelen encontrar con mayor frecuencia en la práctica veterinaria. Estas garrapatas están conformadas por diversos géneros y especies, diferenciándose por características morfoanatómicas inherentes a cada especie, actualmente se cuenta con pruebas moleculares que facilitan la identificación y clasificación de estas, ayudando, además, a conocer la distribución de las garrapatas en gran parte del mundo. Sin embargo, la biología de las garrapatas sigue siendo aún complicado de entender, sumado a que nuevos hallazgos están contribuyendo a conocer mejor su fisiología y el efecto del cambio climático, que amplía la distribución de ciertas especies de garrapatas alrededor del mundo. Por lo tanto esta revisión presenta aspectos relacionados a la clasificación de las especies de las garrapatas basado en estudios morfológicos y moleculares, fisiología y epidemiología en animales de compañía. Contribuyendo además, con evidenciar la situación epidemiológica en el país, que es aún poco investigada, pero que, con las herramientas que se cuentan en la actualidad, se podrán tomar decisiones para el control del vector y de las enfermedades que estas transmiten. Las conclusiones resumen el estado de la investigación y los ámbitos más importantes para el estudio de las garrapatas.
Plan de negocios para venta de productos agrícolas obtenidos en un invernadero en Lima Metropolitana
Authors:   , Quispe Motta Daniel Jesús , Vera Calero Daniel Alejandro
Published: March 2023

El presente trabajo pretende ser el Business Plan que valide la viabilidad del proyecto Bioinvernadero financiado con recursos propios y por parte de entidades terceras; consideramos la localidad de Santa María del Mar para este fin. El objetivo de dicho proyecto es contribuir con la producción de frutas y vegetales de invernadero con fines comerciales, potenciando el sector agroindustrial y proporcionando al mercado opciones saludables de alimentos libres de contaminantes. Este proyecto ha sido desarrollado bajo el análisis respectivo y considerando las aristas necesarias para optimizar los procesos que permitan su puesta en marcha y sostenibilidad en el tiempo. En este convergen aspectos de estudio de mercado, estratégicos, operacionales, de recursos humanos, de marketing y aspectos financieros. Consideramos que este modelo de negocio es viable y puede replicarse en diversos proyectos relacionados. Asimismo, es de carácter ambiental y contribuirá con el entorno local compartiendo buenas prácticas agrícolas con la comunidad interesada.
Frecuencia de neumonia complicada por Neumococo Serotipo19 A en pacientes menores de 05 años anteriormente vacunados con la vacuna trecevalente en el 2017 al 2020 en la DIRIS Lima Este
Authors:   , Constantini Gallo Marianna Nancy
Published: March 2023

La neumonía es un factor predominante como causal de morbilidad y mortalidad en países de menor desarrollo, como el nuestro, ya que se ha evidenciado que en estas poblaciones la tasa de letalidad puede llegar hasta un 60%. El principal causante de neumonía en edad pediátrica se debe a la bacteria Estreptococo Pneumoniae (EP). Una buena cantidad de programas de vacunación han incorporado con acierto la vacuna antineumocócica, contribuyendo así a la disminución de la incidencia de enfermedad neumocócica invasiva, neumonía, otitis media y enfermedad neumocócica asociada a resistencia antibiótica especialmente en niños. Sin embargo, será necesario observar en las vacunas si se produce aumento en los serotipos no presentes inicialmente. Objetivo: Conocer la frecuencia de Neumonías complicadas por Neumococo 19 A en pacientes menores de 5 años, anteriormente vacunados con la vacuna Trece Valente entre el 2017 y 2020 en la DIRIS Lima Este, tomando como referencia los casos de neumonía complicada registrado en la SE4, meses de Enero de los respectivos años del periodo elegido en el presente estudio. Metodología: Estudio observacional, descriptivo, transversal y retrospectivo. Se revisarán 143 Historias clínicas de niños menores de 5 años en condición de pacientes con neumonía complicada por neumococo 19A, anteriormente vacunados con la vacuna Trece Valente entre el 2017 y 2020 en los siete distritos de la DIRIS Lima Este.
Asociación entre el patrón de resistencia a los antimicrobianos en pacientes con urocultivo positivo para Escherichia coli y la recurrencia de infecciones del tracto urinario en el Hospital Nacional Edgardo Rebagliati Martins durante los años 2013-2016
Authors:   , Ormeño Román María José , Ormeño Román María de los Ángeles
Published: March 2023

Objetivo: determinar la asociación entre la resistencia a antibióticos y la ITU recurrente (rUTI) en pacientes con urocultivos positivos por Escherichia coli en un hospital peruano. Pacientes y métodos: Utilizando un diseño de estudio de cohorte retrospectivo, analizamos todos los casos de pacientes con urocultivo positivo por E. coli que acudieron al Hospital Nacional Edgardo Rebagliati Martins (HNERM), Lima, Perú, del año 2013 a 2016. Los niveles de susceptibilidad antimicrobiana fueron testados por Microscan e interpretados según la guía CLSI. Para evaluar la asociación entre la resistencia a los antibióticos y la rUTI, modelamos el tiempo hasta la recurrencia, ajustando dos modelos de regresión de Cox, uno usando el recuento de familias resistentes a los antibióticos y otro usando la resistencia a múltiples fármacos (MDR) como predictores de interés, respectivamente. Resultados: Analizamos un total de 8553 casos positivos a al menos un episodio de ITU, incluidos 963 con rITU. Entre ellos, estimamos la frecuencia de rUTI del 11,3% (intervalo de confianza [IC] del 95%: 10,6% -12,0%). La frecuencia estimada de MDR fue del 46,5% (IC del 95%: 45,5% -47,6), extensamente resistente a los medicamentos (XDR) del 24,3% (IC del 95%: 23,4% –25,2%) y, finalmente, la presencia de productores de betalactamasas de espectro extendido (BLEE) del 42,5% (IC del 95%: 41,9% –44,0%)...
Desempeño laboral y calidad de atención en los servicios de publicidad Registral N° IX Sede Lima de la Superintendencia Nacional de los Registros Públicos
Authors:   , Estrada Tuero Marisol Janet
Published: March 2023

La presente investigación formula el problema sobre la relación presente entre el desempeño laboral de los trabajadores y la calidad de atención en el servicio de publicidad registral de la Zona Registral N° IX oficina que integra la Superintendencia Nacional de los Registros Públicos, 2019. Y como objetivo general se planteó identificar el vínculo entre la variable independiente desempeño laboral y la variable dependiente calidad de atención en el servicio de publicidad registral de la Zona Registral No IX perteneciente a la Superintendencia Nacional de los Registros Públicos, 2019. La investigación se ha realizado mediante un planteamiento no experimental, con nivel correlacional, de corte transversal, con el propósito de identificar el vínculo entre la variable independiente desempeño laboral y la variable dependiente calidad de atención y si esta es significativa. Asimismo, se trata de una investigación tipo básica, y enfoque cuantitativo. La población objetivo está conformada por 315 empleados del servicio de publicidad registral y el grupo de muestra es 173 personas. En el estudio, se ha identificado una dependencia alta y significativa que incluye un coeficiente, Rho de Spearman = .882 y además el nivel de relevancia es p= .000 < .05.
Diagnostic and Therapeutic Approaches for Endometriosis: A Patent Landscape
Authors: Singh Maninder , Jassal Reena , Khetarpal Preeti
Published: March 2023

Objective

The aim of this review is to analyse the patent filings and to systematize the main technological trends in patent protection for the diagnosis and therapeutics for Endometriosis. Patent literature has also been explored to find out active inventors and applicants in this field.

Methodology

Patent Search is carried in the freely accessible Patent search databases namely Patentscope using Various combinations of the key words “Endometriosis OR Adenomyosis” AND “Diagnostic OR Therapeutics”, were used along with wildcard search query in the “Title”, “Abstract” and “Descriptions” fields.

Results

Patent search revealed one hundred forty-four patents which describes invention for endometriosis diagnostic and therapeutic purposes. These patents includes twenty six patent applications in diagnostic utility, one hundred sixteen patents applications under the therapeutic approaches and forty three patent applications describes under traditional medicines for endometriosis.

Conclusion

Hormonal alteration is the most active field of invention followed by surgical interventions. Although there is a general trend of increase in patent applications has been observed but there is slight decrease in the recent years has also been observed.
The effect of manipulating glucuronic acid biosynthetic pathway in Bacillus subtilis strain on hyaluronic acid production
Authors: Afrasiabi Shadi , Zanjani Fatemeh Sadat Amjad , Ahmadian Gholamreza , Cohan Reza Ahangari , keramati malihe
Published: March 2023

Hyaluronic acid (HA), composed of glucuronic acid (GlcUA) and N-acetyl glucoseamine (GlcNAc), is a versatile biopolymer with high commercial value and innumerous physiological roles and pharmaceutical applications. The hasA gene is responsible for HA biosynthesis by Streptococcusstrain as a natural producer. The hasB and hasC genes are also responsible for GlcUA precursor biosynthesis. In the present study, S. equisimilis hasA gene; B. subtilis tuaD and gtaB genes for GlcUA precursorsenhancement, and vgb gene coding bacterial hemoglobin as an oxygen provider were used to engineer the B. subtilis strainfor HA production. RBSHA (hasA), RBSHA2 (hasA/tuaD/gtaB), and RBSHA3 (hasA/tuaD/gtaB/vgb) strains were developed and confirmed through genotype and phenotype analysis. After HA production and purification, FTIR spectroscopy confirmed the HA structures produced by the strains. HA assay showed the highest HA titer for RBSHA3 (2.1 ± 0.18 mg/ml) and then RBSHA2 (1.9 ± 0.03 mg/ml), and RBSHA (0.6 ± 0.14 mg/ml). Statistical analysis indicated there is no significant difference in HA titer between RBSHA2 and RBSHA3 strains (p-value > 0.05), however, these strains produced HA approximately 4-fold higher than that of RBSHA strain. Agarose gel electrophoresis showed the same molecular weight (< 30 kDa) of produced HA. Dynamic light scattering (DLS) revealed all HA polymers had a relatively low polydispersity index (PDI < 0.5). Our study demonstrates the successful GlcUA biosynthetic pathway engineering strategy in improving HA yield by recombinant B.subtilis, metabolically-robust, and industrially potential strain.
To assess the impact of problem lists' accuracy in electronic health records on clinical decision-making: protocol for a randomized controlled trial
Authors: Klappe Eva Susanne , Heijmans Jarom , Groen Kaz , Cornet Ronald , de Keizer Nicolette F
Published: March 2023

Background Problem-oriented electronic health record (EHR) systems, with complete, coded and up-to-date (i.e. accurate) problem lists, aid healthcare providers to track patient’s health status and make better decisions, as problem lists can provide useful summaries of important health issues. Unfortunately, problem lists are still incomplete and out-of-date (i.e. inaccurate). That is, among others, because providers are under time pressure, prefer writing free-text notes and are often unwilling to update problem lists, unless receiving returns for their effort. This study aims to assess the impact of accuracy of problem lists in EHRs on clinical decision-making.

Methods In a laboratory setting we will perform a crossover randomized controlled trial in which we will recruit individual Dutch healthcare providers on-site at Amsterdam University Medical Centers. Participants will be presented with two records of two patients (A and B): one with an accurate and one with an inaccurate problem list, created in a training environment of the EPIC EHR in agreement with clinical experts. Randomization determines which record will have the accurate problem list. Participants are informed that EHR-usage is investigated and do not know which record has an accurate or inaccurate problem list. Participants will provide a motivated (Yes/No) answer on whether prescribing medication X and medication Y is appropriate. Medication Y cannot be prescribed in both patient records, due to contraindicated diagnosis (A) and diagnosis-related medical history (B). Medication X serves as a control question, and is contraindicated based on an allergy, which is equally documented in the records. The primary outcome measure is the correctness of the motivation for the correct answer of medication Y. Secondary outcome measures are: correctness of X (and Y) with the right motivation(s); total time to answer X and Y where the motivation for Y (and X) is correct. Time stamps are registered between opening the question and confirming two Yes/No answers. Proportion of retrieved correct answers and time to answer(s) retrieval will be compared by Chi-square, McNemar tests and Log Rank survival analyses. Alternative analytical models will be applied if necessary.

Discussion If accurate problem lists lead to faster and better decision-making resulting in better patient outcomes, one may be motivated to develop future policies for this area and healthcare providers may be persuaded to use and update problem lists, also leading to improved data quality and opportunities for reuse.
Plastic mulching reduces surface-soil microbial biomass carbon and structural stability in a pear orchard
Authors: Wu Yang , Zhao Zhi , Sun Mingde , Liu Songzhong
Published: March 2023

Background and aims Plastic mulching (PM) can improve soil aggregate stability and microbial activity, thereby preventing soil degradation in crop fields, which are different from the conditions in the orchard. However, little is known about the response of surface-soil microbial and structural stability to PM applied in orchards. Methods An experiment was conducted from 2017 to 2020 in a pear orchard. Two treatments consisted of no mulching and plastic (black woven polypropylene fabric) lying under a pear tree canopy. Results Results showed that the soil microbial biomass carbon content (MBC), organic carbon content (SOC), and ratio of MBC/SOC of PM treatment declined by 49.2%, 13.4%, and 41.3%, respectively. In particular, the significant decline in ratio of MBC/SOC indicated that SOC content would decrease in the future. Mean weight diameter and geometric diameter of soil aggregates in PM treatment decreased by approximately 20%, as compared with no mulching. The decrease in MBC, SOC and soil aggregates stability under PM were mainly resulted from the reduction in root exudate production and root-associated symbionts, causing by the absence of grass roots. These can be proved by the soil gas exchange and concentration. Soil respiration and mineralization were decreased by PM, but soil oxygen concentration at 10 cm depth in PM were improved, due to the oxygen consumed by microbial in no mulching treatment and low gas exchange at this depth. Conclusions Unlike the crop field, protecting the surface soil in orchard from degradation under long-term plastic mulching application is worth considering.
Dual trigger for final oocyte maturation in expected normal responders with a high immature oocyte rate: A randomized controlled trial
Authors: Yan Meng-Han , Zhang Qian-Qian , Yang Wen-Xiu , Sun Zhen-Gao , Song Jing-Yan
Published: March 2023

Purpose
To evaluate whether dual trigger could improve reproductive outcomes in women with low oocyte maturation rates compare to human chorionic gonadotropin (hCG) trigger.
Methods
This study included expected normal ovarian responders younger than 40 years old whose immature oocyte rate in the previous cycle was more than 50% at the reproductive center from July 2021 to January 2023. A total of 73 patients were enrolled at trigger, including 34 in the hCG trigger group and 39 in the dual trigger group (co-administration of gonadotrophin releasing hormone (GnRH) agonist and hCG, 40 and 34 hours prior to oocyte retrieval, respectively). The primary outcome was oocyte maturation rate.
Results
There was no significant difference in the number of oocytes retrieved between the two study groups, but the oocyte maturation rate was higher in dual trigger group (84.0% [14.0%] vs. 55.5% [19.8%], P < 0.001). Moreover, there were also higher cumulative pregnancy rate (69.4% vs. 40.0%, P = 0.035) and cumulative live birth rate (66.7% vs. 36.0%, P = 0.022) in dual trigger group.
Conclusion
For normal responders with low oocyte maturation rates, the dual trigger may be more effective than the conventional hCG trigger.
Trial Registration
Chictr.gov.cn, identifier: ChiCTR2100049292
MiRNA-625-3p as novel biomarker promotes cell proliferation and metastasis of lung adenocarcinoma by targeting KLF9
Authors: Qu Yunhui , Si Qiufang , Yang Ting , Liu Man , Du Renle , Ji Longtao , Zhang Xue , Su Jiao , Sun Hao , Wang Longzhi , Zhao Chunling , Liu Jingjing , Dai Liping , Ouyang Songyun
Published: March 2023

Background. MicroRNAs (miRNAs) are emerging as potential blood-based biomarkers and involved carcinogenesis for various cancers, including lung adenocarcinoma (LUAD).
Methods. In the present study, microarray was used to screen 2,549 miRNAs in serum samples (7 LUAD and 7 healthy samples). qRT-PCR was used to validate the expression of miRNA in serum samples from 30 LUAD and 30 normal individuals. Area under the receiver operating characteristic curve (AUC) was performed to evaluate diagnostic capability of miR-625-3p. Cell counting kit-8 assay (CCK-8) and Transwell assays were used to explore cell proliferation, migration and invasion. Bioinformatics prediction was applied in the search for the target genes of miR-625-3p. qRT-PCR, western blot and dual luciferase assay were used to validate the target gene of miR-625-3p. A xenograft tumor model was established to evaluate cell proliferation in vivo.
Results. MiR-625-3p was the most increased miRNA expressed in LUAD serum samples according to microarray analysis, which was verified in an independent serum sample, as well as in tissues based on TCGA database. And serum miR-625-3p provided a high diagnostic accuracy of LUAD (AUC=0.790, 95%CI:0.6640-0.9152). Functionally, miR-625-3p promoted LUAD cell proliferation, migration and invasion both in vivo and in vitro. Mechanistically, we identified KLF9 as an essential direct target of miR-625-3p, miR-625-3p promoted cell proliferation and metastasis of LUAD by targeting KLF9.
Conclusions. Our study identified that miR-625-3p plays an oncogenic role in LUAD, which promoted proliferation and migration through targeting KLF9. MiR-625-3p might be a potential novel diagnostic biomarker and target for LUAD therapy.
Delineation of Functional Changes and Associated Cortical Transcriptomic Profiles for Autism Spectrum Disorders
Authors: Wang Jiaojian , Li Yanling , Li Rui , Yang Jia , Ma Yingzi , Su Jing , Yu Xiaohui , Chen Kexuan , Lu Fengmei , Chen Yongchang
Published: March 2023

Background: Although abnormal functional activities or connectivities in individuals with autism spectrum disorders (ASD) have been reported, yet, there is still lack of consistent findings of altered functional activities or connectivities and its associated genetic basis for ASD.
Methods: Here, we aimed to identify consistent brain functional alterations and the biological genetic mechanisms in children and adults with ASD using a large sample of resting-state functional magnetic resonance imaging data from the Autism Brain Image Data Exchange dataset (ABIDE). Multi-site based meta-analyses were first employed and identified abnormal brain activity, local and global functional connectivities primarily localizing in frontal-temporal-occipital regions and default mode network (DMN) in individuals with ASD.
Results: The significant correlations between the aberrant brain regions and clinical phenotypes related to social functions in ASD were found. Importantly, brain-wide transcriptome-neuroimaging spatial association analyses and the follow-up enrichment, tissue-specific expression, and protein-protein interaction analyses were conducted and identified the underlying cellular and molecular mechanisms of ASD.
Conclusions: Collectively, our findings link molecular and functional changes in ASD and provide new evidence for neuropathology and a potential precious treatment target for ASD.
Uptake and Barriers to Cervical Cancer Screening among Human Immunodeficiency Virus-Positive Women in Sub Saharan Africa: A Systematic Review and Meta-Analysis
Authors: Mengesha Meresa Berwo , Chekole Tesfaye Temesgen , Hidru Hagos Degefa
Published: March 2023

Background
Cervical cancer is a leading cause of disability and mortality among women in Africa. Despite significant correlation between HIV/AIDS and cervical cancer, unacceptably low coverage of uptake of cervical cancer screening among Human Immunodeficiency Virus-positive women in Sub-Saharan Africa. Individual primary studies are limited in explaining the patterns of uptake of cervical cancer screening. Hence, this review considers the uptake of cervical cancer screening and its barriers among Human Immunodeficiency Virus-positive women in Sub-Saharan Africa.
Methods
We systematically searched articles published up to December 31st, 2019 from databases of PubMed, Cochrane Library, POP LINE, Google Scholar, African Journals Online and JURN. Quality of included articles was assessed by using the Newcastle-Ottawa Scale and the coverage of uptake of cervical cancer screening was pooled after checking for heterogeneity and publication bias. The random effect model was used and sub-group analysis estimate was done by countries.
Results
Twenty-one studies comprised of 20,672 Human Immunodeficiency Virus-Positive women were included. Applying random effect model, the overall cervical cancer screening uptake among this group of women in Sub Saharan Africa was estimated to be 30% (95% CI: 19, 41, I2 = 100%). The main barriers to uptake of cervical screening to include: poor knowledge about cervical cancer and screening, low risk perception of cervical cancer, fear of test result and fear of screening as painful, lack of access to screening services, high cost of screening service, and poor partner attitude and acceptance of the service. The perception of an additional burden of having a cervical cancer diagnosis was found to be a unique barrier among this population of women.
Conclusion
The review revealed that cervical cancer screening uptake is low due to poor knowledge about cervical cancer and screening, low risk perception of cervical cancer, Fear of test result and fear of screening, lack of access to screening services, high cost of screening services and poor partner attitude and acceptance of the service. Besides the above, perception of an additional burden of having a cervical cancer was found to be a unique barrier for these group of population.
The efficacy and safety of conscious sedation applied with midazolam in the transesophageal echocardiography procedure
Authors: Harbalıoğlu Hazar , Yıldırım Abdullah , Koca Fadime , Nacar Halil , Ucak Dilek , Koca Hasan
Published: March 2023

Objective The aim of this study was to compare the anaesthesia parameters, procedure duration, and the effect on hemodynamics and clinical parameters during the procedure in patients undergoing transesophageal echocardiography (TEE) with conscious sedation applied with midazolam or propofol. Methods This cross-sectional study included 401 patients (198 males, 203 females, mean age 52.9 ± 14.8 years) applied with TEE in our clinic. The demographic, clinical and laboratory parameters of the patients were recorded before the procedure. A record was made of pre-procedure ASA score and basal SaO2, the time to sedation to TEE, TEE duration, time to recovery, and during the procedure the minimum SaO2, the need for non-invasive mechanical ventilation (NIMV), the change in systolic and diastolic blood pressure (SBP and DBP), O2saturation change, and pulse change. The data were compared between the patients in two groups according to the conscious sedation agent used; midazolam and propofol. Results The demographic, clinical, and laboratory data of the midazolam and propofol groups were found to be similar (p > 0.05 for each). The procedure duration, and time to recovery were determined to be significantly shorter in the midazolam group than in the propofol group, and the time to onset of the sedation effect was significantly longer (p < 0.05 for each). Of the respiratory parameters, the minimum SaO2during the procedure, the absolute change in O2saturation, and the need for NIMV were determined to be significantly lower in the midazolam group (p < 0.05 for each). The absolute pulse and change in SBP and DBP values were found to be significantly higher in the propofol group (p < 0.05 for each). Conclusions The study results demonstrated that conscious sedation applied with midazolam during TEE can be applied with a shorter procedure duration and better hemodynamic and clinical results compared to sedation with propofol.
Enumeration module for human visual system: a recurrent on-center off-surround neural network approach
Authors: Verma Bhavesh , Sengupta Rakesh
Published: March 2023

Humans have an inherent ability to visually perceive small numerosities (the cardinality of a set). According to several studies, the LIP and other IPS regions of the brain include the neurological substrates for the processes related to numbers. Computational models play a crucial role in investigating the computations and dynamics behind the perception of numbers. Existing models of number perception simulate a few of the fundamental aspects of number perception, such as size and distance effects. However, most models usually work for a limited range of numbers and lack explanations for important behavioral characteristics like adaptation effects.In this study, we use a network of neurons with self-excitatory and mutual inhibitory properties to build a computational model of number perception. We assume that the network's mean activation at steady-state can encode numerosity when it increases monotonically with Setsize (the input to the network). By optimizing the total number of inhibition strengths required so that the combined monotonic regions cover the full stretch of numbers, we get three ranges of numbers (1:4, 5:17, and 21:50). This division of numbers into three parts closely matches the elbows in numerosity perception discovered in behavioral studies. Later in the study, we devised a method to decode the mean activation into a continuous scale of numbers ranging from 1 to 50. Furthermore, we suggest a mechanism for selecting inhibition strength based on current inputs, allowing the network to work for the entire range of numerosities. Our model provides novel perspectives on how our brain can generate various behavioral phenomena, such as the influences of continuous visual attributes and adaptation effects on perceived numerosity
Purification of Acetylcholinesterase from Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) and Management of Control of The Pest Through Inhibition of The Enzyme
Authors: Dinçer Barbaros , AKPINAR Havva
Published: March 2023

Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) has brought about agricultural harm throughout the Eastern Black Sea coastline since 2017. It continues to come to a threat because there are no adequate studies on managing this pest. One of the major rein strategies of insecticides is acetylcholinesterase (ACHE) inhibition. Therefore, this study aims to investigate an alternative way to struggle H. halys by inhibiting ACHE. The ACHE was purified from H. halys using edrophonium-Sepharose 6B affinity chromatography and characterized by examining some kinetic properties. The molecular weight of the purified enzyme was determined by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and natural polyacrylamide gel electrophoresis. In addition, the inhibitory effects of tacrine and edrophonium chloride, and water extracts of olive leaf, walnut leaf and alder leaf on this ACHE were investigated. The acetylcholinesterase was purified 403-fold with an 83.3% yield. H. halys ACHE was found to have six subunits and a molecular weight of approximately 350 kDa. The ACHE’s Km, Vmax, and kcat values were assigned to be 0.02 ± 0.006 mM, 3,333.3 ± 481 EU.mg protein− 1, and 1070.2 ± 184 min− 1, respectively. All inhibitors highly inhibited of activity of h.halys ACHE. Especially, the fact that the water extracts of these plants are effective in ACHE inhibition is significant due to being environmentally amicable pesticides that may be benefited in the struggle with the pest.
Metabolomic Evaluation of Air Pollution-related Bone Damage and Potential Mediation
Authors: Prada Diddier , Rexrode Kathryn , Kalia Vrinda , Kooperberg Charles , Reiner Alexander , Balasubramanian Raji , Wu Hui-Chen , Miller Gary , lonita-Laza Iuliana , Crandall Carolyn , Cantu-de-Leon David , Liao Duanping , Yanosky Jeff , Stewart James , Whitsel Eric , Baccarelli Andrea
Published: March 2023

Ambient air pollution has been associated with bone damage. However, no studies have evaluated the metabolomic response to air pollutants and its potential influence on bone health in postmenopausal women. We analyzed data from WHI participants with plasma samples. Whole-body, total hip, femoral neck, and spine BMD at enrollment and follow-up (Y1, Y3, Y6). Daily particulate matter NO, NO2, PM10 and SO2 were averaged over 1-, 3-, and 5-year periods before metabolomic assessments. Statistical analyses included multivariable-adjusted linear mixed models, pathways analyses, and mediation modeling. NO, NO2, and SO2, but not PM10, were associated with taurine, inosine, and C38:4 phosphatidylethanolamine (PE), at all averaging periods. We found a partial mediation of C38:4 PE in the association between 1-year average NO and lumbar spine BMD (p-value: 0.032). This is the first study suggesting that a PE may partially mediate air pollution-related bone damage in postmenopausal women.
Protein extraction from excess sludge by barium hydroxide hydrolysis process
Authors: Hui Wenlong , Zhou Jiti , Jin Ruofei
Published: March 2023

Protein could be extracted effectively from excess sludge by barium hydroxide (Ba(OH)2) hydrolysis process. During the extraction process, insoluble protein in sludge was turned into soluble protein, and part of the soluble protein were hydrolyzed into peptide, amino acids, water, carbon dioxide and ammonium. To obtain the maximum concentration of soluble protein, the effects of barium hydroxide, temperature and time on the hydrolysis degree of the sludge protein and protein concentration were investigated. Under optimal conditions of 0.2 M Ba(OH)2, 110 ℃ of the reaction temperature, and 4 h of hydrolysis time, the degree of sludge protein hydrolysis and the protein concentration was 79.10% and 1106.11 mg/L, respectively. Through building a SPSS multiple nonlinear regression model, the optimal protein concentration was calculated. Barium ions were converted into barium sulfate precipitates by adding an equal amount of sulfuric acid in the hydrolysate. Then the precipitates were washed with 0.1 M NaOH to obtain pure barium sulfate and protein solution. Protein was separated and obtained from sludge hydrolysate by centrifugation and filtration, which could be used as a wood binder or foaming agent.
The Mathematical Relationship Between Certainty and Probability: The Invert of The Shannon Information Entropy Curve
Authors: Khalsa Sundri
Published: March 2023

The relationship between certainty-level and probability-level has not been defined in mathematics literature. Some say it is pointless to define because certainty is absolute. Others imply certainty-level and confidence level are interchangeable and consequently imply certainly-level is the probability that a randomly-produced range of values called “confidence interval” contains the true value of a population parameter. Many erroneously imply certainty-level and probability-level are interchangeable. Per the following-survey question given to approximately 50 Ivy-League-graduate students, “If someone [is] 50% certain of something, what probability percentage would that mean?”—the nearly-universal answer was 50% probability. No one chose the correct answer— 89% probability. Others offer an absurd definition that would mean a ‘reasonable degree of medical/scientific certainty’ is any value greater than 0% certainty. I demonstrate the relationship is defined by the invert of the Shannon-information-entropy-curve/formula. The definitions of certainty-level (and uncertainty-level) are important to acknowledge and formalize in mathematics literature because professions that make life-and-death decisions based-on the terms have demonstrated confusion and erroneous understanding of them. If the mathematics community chooses to define certainty-level as the mathematics-community definition of confidence level, that definition would defy both the rational-language meaning of certainty-level and the meaning of the invert of the Shannon-information-entropy-formula.
Bioinformatics-Based Identification of Tumor Immune Invasion- Related prognostic Biomarkers in pancreatic cancer
Authors: Chen Minxue , Wang Xingang , Li Xiaojun , Wei Qiuya , Wang Yuebin , Zhou Xinyuan , Fan Yong , Wang Chen
Published: March 2023

Background: The diagnosis and treatment of pancreatic adenocarcinoma (PAAD) remain clinically challenging, and new molecular markers for prognostic assessment and targeted therapy are urgently needed. The tumor microenvironment (TME) and immune invasion play an important role in pancreatic cancer development and progression. Therefore, immunotherapeutic strategies based on the TME and immune invasion may have important clinical value.
Methods: In this study, We extracted transcriptome and clinicopathological data for 179 PAAD samples from the TCGA database and evaluated the immune composition, stromal composition, and infiltrating immune cell landscape in the tumor samples using the Estimation of Stromal and Immune cells in malignant Tumors using Expression data (ESTIMATE) and CIBERSORT algorithms. Then, we identified relevant differentially expressed genes (DEGs) and performed functional annotation and prognostic correlation analysis to identify prognostic biomarkers for pancreatic cancer. Finally, the correlation between biomarkers and tumor immune invasion was analyzed to reveal the molecular immune mechanism of pancreatic cancer.
Results: As the results, we discovered The immune score, stromal score and ESTIMATE score were correlated with the clinicopathological characteristics of patients with PAAD, The results of DEGs, protein-protein interaction (PPI) network and Cox regression analyses confirmed that CXCL10 and CXCL11 were clinically relevant prognostic factors involved in the PAAD TME and that the content and activity of various immune cells were correlated with the expression levels of CXCL10 and CXCL11, CXCL10 and CXCL11 are novel biomarkers of the TME and immune cell infiltration in pancreatic cancer
Conclusions: This study systematically elucidates the possible immune-related molecular mechanisms of pancreatic cancer for the first time. It also provides prognostic clues for early diagnosis of pancreatic cancer, thus providing assistance for immunotherapy of pancreatic cancer.
The prognosis of elderly patients with acute ST-segment elevation myocardial infarction in Northeast China is poor
Authors: Li Bing , Zhang Yaoting , Cai He , Zheng Yang
Published: March 2023

This study evaluated the prognosis in elderly patients with ST-segment elevation myocardial infarction (STEMI) in Northeast China and found key factors to improve prognosis. A total of 480 STEMI patients who underwent primary percutaneous coronary intervention (PCI) were enrolled. Outcomes of in-hospital and long-term were collected. Patients were assigned into younger group (below 60 years old) and elderly group (equal or above 60 years old). Elderly group had higher rates of females and hypertension, lower incidences of dyslipidemia and smoking than the younger group. Elderly patients were more likely to have complications of heart failure, longer length of stay and less liable to receive β-blockers. Clinical outcomes showed the rate of 6-month, 12-month, and 18-month MACEs were higher in elderly group (P < 0.05 for all comparisons). Post - procedure TIMI 3 flow was an independent factor and was positively correlated with favorable outcomes. The elderly group presented significantly reduced MACEs-free survival and death-free survival than the younger group (Log-rank test, P < 0.05). We found advanced age was associated with a significantly increased risk of MACEs during follow-up than the younger patients and post-procedure TIMI 3 flow was positively related to favorable long-term outcomes in elderly patients.
Enhancing Ceria Slurry Performance for Shallow Trench Isolation Chemical Mechanical Polishing through Non-Ionic Surfactant Addition
Authors: Zhang Lifei , Xie Lile , Lu Xinchun
Published: March 2023

Chemical mechanical polishing (CMP) is a committed step in the manufacturing of integrated circuits, especially in the fabrication process of shallow trench isolation (STI) structures. Ceria (CeO2) slurry is widely used in the STI CMP process, while it is vulnerable to causing surface defects owing to particle agglomeration, such as scratches and abrasive residues. Furthermore, CeO2 slurry faces the challenge of low polishing removal selectivity between silicon dioxide (SiO2) and silicon nitride (Si3N4) surfaces. In this study, we investigated the effects of various non-ionic surfactants and different pH levels in CeO2 based slurries on material removal rates, removal selectivity, and surface qualities of polished wafers. Two of the studied non-ionic surfactants that make CeO2 slurries disperse better were selected through sedimentation experiments, which were polyethylpyrrolidone (PVP-K30) and polyethylene glycol, respectively. Subsequently, polishing experiments and atomic force microscopy characterization tests were conducted to illustrate the effects of the selected surfactants at different pH conditions. To further explore the underlying mechanism, the reaction of surfactants on SiO2 and Si3N4 wafers was explained using surface adsorption tests, thermogravimetry experiments, zeta potential measurements, and chemical valence bond structure analysis. As a result, it can be concluded that the performance of ceria slurries used in STI CMP process can be improved by the addition of non-ionic surfactant PVP-K30.
Research on liver cancer segmentation method based on PCNN image processing and SE-ResUnet
Authors: Zang Lan , Li Jianrong , Wu XinXin , Zhang Kun , Shen Chong
Published: March 2023

As one of the malignant tumors with high mortality, the initial symptoms of liver cancer are not obvious. In addition, the liver is the largest internal organ of the human body, and its structure and distribution are relatively complex. Therefore, in order to help doctors judge liver cancer more accurately, this paper proposes a variant model based on Unet network. Before segmentation, the image is preprocessed, and Pulse Coupled Neural Network(PCNN) algorithm is used to filterthe image adaptively to make the image clearer. For the segmentation model, the SE module is used as the input of the residual network, and then its output is connected to the Unet model through bilinear interpolation to perform the down-sampling and up-sampling operations. The Lits and collected liver cancer images are combined into a new data set for training. The results show that this method has better segmentation performance and accuracy than the original Unet method, and the dice coefficient, mIou and other evaluation indicators have increased by at least 2.1%, which is a method that can be applied to cancer segmentation.
An accuracy study of the AL/CR ratio in assessing different degrees of hyperopia in children aged 3-6 years
Authors: Wang Yang , Xia Yun , Xie Xuanxuan , Zhu Xiaohui , Yang Jing , Ouyang Lijuan , Pi Lianhong
Published: March 2023

Purpose: To assess the association between different hyperopia levels and the axial length/radius of corneal curvature (AL/CR) ratio and check if hyperopia levels can be assessed by the AL/CR ratio, and provide a basis for hyperopia screening in preschool children.
Methods: The spherical equivalent refraction (SER), axial length (AL), and corneal refractive power (K) were obtained through cycloplegia optometry, IOL-Master on children aged 3-6 years, and the AL/CR ratio was calculated. They were categorized into four groups based on the SER: physiological, mild, moderate, and high hyperopia. The data were analyzed utilizing SPSS 23.0.
Results: The AL/CR ratio in the high hyperopia group (2.60 ± 0.08) compared to the moderate hyperopia group (2.72 ± 0.08), and the mild hyperopia group (2.83 ± 0.07) was statistically significant (F = 508.125, P = 0.000). The association between SER and AL/CR ratio was higher than the AL and the CR (R = -0.873, R = -0.738, R = 0.374, P = 0.000), and it became greater with the increasing degree of hyperopia (R = -0.284, R = -0.478, R = -0.401, R = -0.637, P = 0.000), with a mild negative correlation in the physiological hyperopia group and a moderate negative correlation in the mild, moderate and high hyperopia groups. The linear regression equation showed that for every 0.1 unit decrease in AL/CR ratio, the SER changed by approximately 1.68 D in the hyperopic direction (R2 = 0.762, P = 0.000). In children aged 3-6 years, when the AL/CR ratio was ≤ 2.80, this suggested SER of approximately ≥ +4.0D.
Conclusions: The AL/CR ratio can be utilized to assess different degrees of hyperopia and as an objective indicator of hyperopia. The AL/CR ratio ≤ 2.80 indicates moderate hyperopia in preschool children.
Study on the relationship between Ferroptosis gene and immune microenvironment in ovarian cancer and related prognostic model construction
Authors: Li chunjin , Gao na , hua Yan rui
Published: March 2023

Ovarian cancer (OC) has a high recurrence rate and insufficient early screening methods, with a 5-year survival rate of less than 50% However, in many cases, simple standard treatment results are not satisfactory. Therefore, more effective treatments are being sought Ferroptosis is a recently discovered form of cell death associated with loss of glutathione peroxidase 4 (GPX 4) activity and accumulation of reactive lipid oxygen species (ROS), which shows great potential for anticancer therapy[1]. The results of immunotherapy for ovarian cancer are not ideal, but some studies have shown that the results of immunotherapy are significantly improved when combined with other treatments. Gene expression profiling allows researchers to explore and elucidate the molecular mechanisms underlying disease at the genomic and transcriptomic levels. integration of genomic technology and bioinformatics analysis Although these tools allow researchers to screen for tumor-associated genes and identify key prognostic factors, single-gene biomarkers that provide effective survival predictions are limited. Multigene-based risk models may provide better prognostic factors to predict patient survival[2]. identify multigene risk score characteristics by bioinformatic analysis and find more potential biomarkers for effective diagnosis and prognosis assessment of ovarian cancer patients. Based on TCGA and GEO transcript expression data and associated clinical data, a scoring system was developed using consensus cluster analysis, univariate Cox regression analysis, and lasso regression and validated against GEO database data. The results of univariate and multivariate risk analysis showed that the score can be used as an independent prognostic marker of ovarian cancer.