bluuebunny/crossref_metadata_2025 huggingface dataset:
Trophic ecology of mesopelagic fishes in the northern and southern Benguela Upwelling Systems revealed through stable isotope patterns
Authors: Duncan SE , Fock HO , Sell AF , Hagen W
Published: October 2023

Mesopelagic fishes are important components of marine food webs as trophic links between primary consumers and higher trophic levels, but their trophic ecology is poorly understood. We used stable isotopes (δ13C and δ15N) to compare the trophic position, trophic enrichment factor, and isotopic niche among feeding guilds of mesopelagic fishes (including myctophids, stomiids) during different seasons in the northern and southern Benguela Upwelling Systems. These subsystems are influenced by differing water masses with various physical and biological properties, which can lead to changes in the marine food web. We aimed to determine if and how the trophic ecology of mesopelagic fishes differs between seasons and subsystems. When separating fishes into guilds based on dietary preferences (zooplanktivores vs. piscivores) and migration patterns (migrating vs. non-migrating), the trophic position was highest during winter in the south for non-migrating piscivores (4.5), but the trophic positions of piscivores (3.2-4.4) were not always higher than those of zooplanktivores (3.1-4.5). This may indicate different isotopic enrichment between species or a very generalist diet from zooplanktivores such as myctophids and their prey. Trophic enrichment factors for δ15N among guilds ranged from 3.8 to 7.5‰ between the baseline and zooplanktivores and from -0.8 to 1.6‰ between zooplanktivores and piscivores. Previously, mesopelagic fishes have been incorporated into food web models in the Benguela Upwelling Systems, but only as a single entity. Elucidating the complex seasonal and regional trophodynamics of various mesopelagic feeding guilds may contribute to more accurate assessments of trophic transfer efficiencies and higher-resolution models in these systems.
SEKRETARIS DAN LINGKUNGAN KERJA
Authors: Risnawati Naniek
Published: October 2023

Abstract. In order to achieve the survival of the company, it is necessary to have a secretary as the right hand of the leader who is in charge of assisting the leadership tasks and being a person who can be relied upon in the company. In order for a company to have a stable and continuously increasing level of profitability, maximum quality work productivity is required. One of the factors that affect employee work productivity is the work environment that exists in a company both in terms of physical and non-physical. This is where the role of the secretary is needed in creating a conducive and supportive work environment and atmosphere..

Keyword: secretary, work environment, work atmosphere

Abstraksi. Untuk tercapainya keberlangsungan hidup perusahaan maka perlu seorang sekretaris sebagai tangan kanan pimpinan yang bertugas membantu tugas-tugas pimpinan serta menjadi orang yang bisa diandalkan dalam perusahaan. Agar perusahaan memiliki tingkat profitabilitas yang stabil dan terus meningkat, diperlukan kualitas produktivitas kerja yang maksimal. Salah satu faktor yang mempengaruhi produktivitas kerja karyawan adalah lingkungan kerja yang ada pada suatu perusahaan tersebut baik dalam hal fisik maupun non fisik. Disinilah peran sekretaris sangat dibutuhkan dalam menciptakan lingkungan dan suasana kerja yang kondusif dan mendukung.

Kata kunci: sekretaris, lingkungan kerja, suasana kerja
THE USE OF PERFORMANCE-BASED ASSESSMENT TOWARD EFL STUDENTSâ€™ LEARNING ATTITUDE
Authors: Siti Amalia Rachmawati
Published: October 2023

Abstract: This study aims to identify the use of performance-based assessment toward the EFL studentsâ€™ learning attitude of how it can be implemented for the EFL students and what are the impacts of using performance-based assessment toward the EFL studentsâ€™ learning attitude. This paper identifies the EFL teachersâ€™ opinions about the implementation and the impact of performance-based assessment for the EFL students. The data analysis of this study is based on the theory about performance-based assessment and studentsâ€™ learning attitudes. The method used in this research is descriptive qualitative research method which deals with data that are in the form of words and not showed as numeric data by using interview data toward two EFL teachers in different level. This study results show that the English teachers have many ways in implementing  performance-based assessment and this implementation brings positive and negative impacts. In clonclusion, each of English teacher has their own way to apply this kind assessment so that it can give the good effect to their studentsâ€™ learning attitude. 

Keywords: Alternative Assessment, Performance-Based Assessment, EFL studentsâ€™ learning Attitudes

 

 

Abstrak: Penelitian ini bertujuan untuk mengidentifikasi penggunaan penilaian berbasis kinerja terhadap sikap belajar siswa EFL tentang bagaimana penerapannya untuk siswa bahasa Inggris dan apa dampak penggunaan penilaian berbasis kinerja terhadap sikap belajar siswa tersebut. Makalah ini mengidentifikasi pendapat guru bahasa Inggris tentang penerapan dan dampak penilaian berbasis kinerja untuk para siswanya. Analisis data penelitian ini didasarkan pada teori tentang penilaian berbasis kinerja dan sikap belajar siswa. Metode yang digunakan dalam penelitian ini adalah metode penelitian kualitatif deskriptif yang berhubungan dengan data yang berupa kata-kata dan tidak ditampilkan sebagai data numerik dengan menggunakan data wawancara kepada dua orang guru bahasa Inggris di jenjang yang berbeda. Hasil penelitian ini menunjukkan bahwa guru bahasa Inggris memiliki banyak cara dalam menerapkan penilaian berbasis kinerja dan penerapan ini membawa dampak positif dan negatif. Kesimpulannya, setiap guru bahasa Inggris memiliki cara masing-masing dalam menerapkan asesmen semacam ini sehingga dapat memberikan pengaruh yang baik terhadap sikap belajar siswanya.

Kata kunci: Penilaian Alternatif, Penilaian Berbasis Kinerja, Sikap Belajar Siswa Bahasa Inggris
KEVALIDAN LKPD BERBASIS PICTURE AND PICTURE MATERI SISTEM PERNAPASAN MANUSIA KELAS VIII SMP/MTs
Authors: Supiandri  , Nur Kuswanti  , Lina Arifah Fitriyah
Published: October 2023

Abstract Science at the junior high school level can be learned as a provision of science knowledge, concepts and skills at the next level. The concept of science learning is attempted by students to understand so that maximum learning outcomes are obtained. The results of observations to students of class VIII SMP stated that teachers in studying students still used conventional methods, students liked group learning methods and discussions, students liked science learning if the teacher used pictures and writing media. The purpose of this study was to develop a cooperative student worksheet (LKPD) based on the picture and picture type on the human respiratory system material for grade VIII SMP / MTs based on validity. The use of this research instrument was in the form of a LKPD validation sheet. The assessment sheet is filled with three validators, namely expert lecturers and science teachers. The aspects assessed were didactic, construction, technical, and LKPD characteristics. The results of the validation of two material expert lecturers and junior high school science teachers showed that the picture and picture type cooperative-based LKPD was very valid to use in the human respiratory system material. The average score obtained from the two subject matter expert lecturers and junior high school science teachers is 3.63. So LKPD is suitable for use as teaching material on the human respiratory system material based on the validation results.

Keywords: Picture and Picture, Human respiratory system, LKPD, Valid

 

 

Abstrak: IPA pada jenjang sekolah menengah pertama dapat dibelajarkan sebagai bekal pengetahuan, konsep dan keterampilan IPA pada jenjang selanjutnya. Konsep pembelajaran IPA diusahakan peserta didik paham dan mengerti sehingga diperoleh hasil belajar maksimal. Hasil observasi kepada siswa kelas VIII SMP, menyatakan guru dalam membelajari peserta didik masih menggunakan metode  konvensional, peserta didik menyukai metode pembelajaran berkelompok dan diskusi, peserta didik menyukai pembelajaran IPA jika guru menggunakan media gambar dan tulisan. Tujuan penelitian ini adalah mengembangkan lembar kerja peserta didik (LKPD) berbasis kooperatif tipe picture and picture pada materi sistem pernapasan manusia kelas VIII SMP/MTs berdasarkan validitas. Penggunaan instrument penelitian ini berupa lembar validasi LKPD. Lembar penilaian diisi tiga validator yaitu dosen ahli dan guru IPA. Aspek yang dinilai yaitu didaktik,  konstruksi, teknis, dan karakteristik LKPD. Hasil validasi dari dua dosen ahli materi, dan guru IPA SMP bahwa LKPD berbasis kooperatif tipe picture and picture sangat valid digunakan pada materi sistem pernapasan manusia. Skor rata-rata yang di peroleh dari dua dosen ahli materi, dan guru IPA SMP yaitu 3,63. Jadi LKPD layak di gunakan sebagai bahan ajar pada materi sistem pernapasan manusia berdasarkan hasil validasi.

Kata kunci: Picture and picture, Sistem pernapasan manusia, LKPD, Valid
PENGEMBANGAN RENCANA PELAKSANAAN PEMBELAJARAN (RPP) MODEL PEMBELAJARAN INKUIRI TERBIMBING UNTUK MELATIHKAN SIKAP ILMIAH MATERI PEMANASAN GLOBAL KELAS VII SMP
Authors: Dian Masidayu Prahastuti  , Nur Kuswanti  , Andri Wahyu Wijayadi
Published: October 2023

Abstract: Based on questionnaires that have been distributed, it is known that the problem found in class VII SMP is a lack of interest in science subjects because the learning process is still a teacher-centered. Students are more interested in my practice-based learning. Referring to the results of the interview, it shows that one of the materials is with a low average value is Global Warming. In order to make it easier for educators to implement a learning system based on conditions and problems in the classroom, a learning device is needed that is in accordance with the conditions in the class. This study aims to develop a quality Guided Inquiry leson plan, as well as to explain the process of developing Guided Inquiry leson plan based on the ADDIE development procedure, and to describe the feasibility of the lesson plan developed based on the validity of the lesson plan. This research design uses ADDIE (Analyze, Design, Development, Implement, and Evaluate). The results of the validation of the validators obtained an average score of 4.7 and were categorized as very valid. So the lesson plan on global warming material based on guided inquiry learning models to train students' scientific attitudes is feasible to use in learning.

Keywords: Global Warming, Guided Inquiry Model, Lesson Plan.

 

 

Abstrak: Bersumber pada angket yang telah disebar diketahui bahwa masalah yang ditemukan pada kelas VII SMP adalah kurangnya minat pada mata pelajaran IPA dikarenakan proses pembelajaran yang masih bersifat teacher center, peserta didik lebih berminat pada pembelajaran yang berbasis praktiku. Jika mengacu pada hasil wawancara menunjukkan bahwa salah satu materi yang memiliki nilai rata-rata rendah adalah materi Pemanasan Global. Guna memudahkan pendidik mengimplementasikan sistem pembelajaran berdasarkan kondisi dan permasalahan di kelas maka dibutuhkan suatu perangkat pembelajaran yang sesuai dengan kondisi di kelas tersebut. Penelitian ini bertujuan untuk mengembangkan RPP Inkuiri Terbimbing yang berkualitas, serta untuk menjelaskan proses pengembangan RPP Inkuiri Terbimbing berdasarkan prosedur pengembangan ADDIE, dan mendeskripsikan kelayakan RPP yang dikembangkan berdasarkan validitas RPP. Desain penelitian ini menggunakan ADDIE (Analyze, Design, Development, Implement, dan  Evaluate). Hasil validasi dari para validator, diperoleh rata-rata skor 4,7 dan dikatergorikan sangat valid. Jadi RPP pada materi pemanasan Global berbasis model pembelajaran inkuiri terbimbing untuk melatihkan sikap ilmiah peserta didik layak untuk digunakan dalam pembelajaran.

Kata Kunci: Pemanasan Global, Model Inkuiri Terbimbing, RPP.
THE USE OF ALTERNATIVE ASSESSMENT FOR UNDERGRADATE STUDENTS
Authors: Lailatul Fitriyah
Published: October 2023

Abstract In recent years, there are more choices to assess studentsâ€™ abilities andunderstanding in language skill. As assessment plays an important role as themeasurement in checking all steps and the results of language learning, this articledescribes a study that examine the use of alternative assessment for Indonesianundergraduate school students applied by English lectures from two differentuniversities. This study uses surveys and interviews to examine (a) the process ofalternative assessment for undergraduate students, (b) the studentsâ€™ response usingalternative assessment, (c) studentsâ€™ result after being assessed by alternativeassessment. So that, the conceptual frameworks taken from this study are: (a)alternative assessmentâ€™s process, (b) alternative assessmentâ€™s benefits, and (c)responsive pedagogy. This study also explores the impact of alternative assessment inassessing students in the classroom. The results of the survey revealed the majority ofteachers felt that the alternative assessment helped them in assessing the students, aswell as evaluating their beliefs and biases, thereby supporting the idea of incorporatingmore exercises and activities into English classes. The findings show that alternativeassessment is necessary to be applied in teaching and learning since by applying this kindof assessment, the students be able to understand and can practice it in their dailyactivity. Alternative assessment also has positive response from students since it isunderstandable to be practiced.
KECEMASAN SISWA SEKOLAH MENENGAH ATAS SAAT PEMBELAJARAN DARING DI TENGAH PANDEMI COVID-19
Authors: Mohammad Muhyidin
Published: October 2023

Abstract: In March 2020 the Indonesian government imposed online learning from home for all school students as a social restriction effort during the COVID-19 pandemic. This study aims to determine the anxiety experienced by students and their efforts to overcome this anxiety. Ten informants from high school students were included in this study. They were given a questionnaire form containing open-ended questions related to the topic of this research. Data from filling out the questionnaire showed that there was studentsâ€™ anxiety when learning online from home which included difficulties in understanding the material, difficulties in carrying out assignments, problems with the internet network, technical problems, and a sense of worry about the next task. In overcoming this anxiety, they do things like the following, namely studying independently, every time doing a task immediately, discussing with teachers and friends, encouraging themselves, also carrying out other activities, among others, watching movies, listening to music, eating and exercising.

Keywordsâ€‹: â€‹â€‹ Anxiety, Online Learning, Covid-19
OPTIMASI PENJADWALAN PERAWAT DI RUANG UGD RSUD LAPANGAN SAWANG MENGGUNAKAN METODE NON-PREEMPTIVE GOAL PROGRAMMING
Authors: Lowryk O. Lahunduitan  , James U.L Mangobi  , Vivian E. Regar
Published: October 2023

The emergency room which requires a full 24-hour nurse certainly needs to pay attention to the right schedule. To get an optimal schedule, it is necessary to divide nurses according to the needs of the right shift. Schedules that are made manually often violate or pay less attention to the applicable rules. Based on the existing rules in the hospital, constraints are divided into main constraints and additional constraints which are modeled and solved using the Non-Preemptive Goal Programming method with the objective function being to minimize the advantages or disadvantages of additional constraints. Furthermore, for the computational process assisted by the LINGO 19.0 application. From the results and discussion, it is found that the optimal schedule using the Non-Preemptive Goal Programming model is fulfilled for every existing constraint.

Keywords: Schedule, Nurse, Goal Programming, Non-Preemptive Goal Programming
OPTIMASI KEUNTUNGAN PADA HOME INDUSTRY ARYADY BAKERY MENGGUNAKAN LINEAR PROGRAMMING
Authors: Rangga R. Mandalika  , James U.L. Mangobi  , Marvel G. Maukar
Published: October 2023

Home industry Aryady Bakery produces three types of bread, namely brown bread, cheese chocolate bread, and moca bread, but the profits are not maximized. In addition, the process of purchasing raw materials for making bread is carried out with estimates without definite calculations. Based on this, a production model is given in the form of linear programming to optimize profits in the Aryady Bakery Home industry. After observing and collecting data, the decision variables from this linear programming problem are obtained, namely the amount of brown bread, cheese and moca to be produced. In addition, the constraint functions for the model are obtained, namely raw materials, electricity, working hours of production machines, and labor hours, and the coefficients of the function variables on the objective function using the advantages of one brown bread, chocolate cheese bread, and moca bread. Furthermore, the profit optimization calculation process uses a linear program with a simple method with the help of LINDO and TORA software. The maximum yield obtained by producing as many as 4260 brown bread, 1200 cheese chocolate bread, and 500 moca bread each month is Rp. 3,600,460.

Keywords: Linear programming, Simpleks, Profit Optimization
LKPD SISTEM PEREDARAN DARAH BERBASIS PENDEKATAN SAINTIFIK
Authors: Qurrata Aâ€™yun  , Lina Arifah Fitriyah
Published: October 2023

The circulatory system is closely related to human life. In this material, students are expected to know the parts of the heart organ related to activities with the frequency of the heartbeat. Characteristics of SMP/MTs class VIII students are able to use logic so that to study the material of the circulatory system, images that represent the existence of the circulatory system are needed, namely in the form of teaching materials using a scientific approach. The use of a scientific approach in the 2013 curriculum aims to create learning according to process standards. The purpose of this study was to describe the LKPD of the circulatory system based on a scientific approach based on its validity and practicality. The method used in this research is the ADDIE development model. The data of this study consisted of data on the validity and practicality of the developed LKPD. The results showed that the LKPD of the circulatory system based on a scientific approach was declared valid and practical.

Keywords: LKPD, Scientific Approaach, Circulatory System
VALIDITAS MEDIA TUAS PADA PEMBELAJARAN IPA MATERI PESAWAT SEDERHANA
Authors: Salafudin  , Noer Afâ€™idah
Published: October 2023

Learning media is an important part of learning activities that serves to help convey messages from various learning sources in an effective and planned manner, so that learning objectives will be achieved. But so far, teachers rarely use learning media, so they have difficulty in conveying material to students. This causes learning activities to seem boring and students often have difficulty understanding the material. This problem usually occurs in materials that require a high level of understanding, such as simple plane materials, especially the discussion of levers. This study aims to develop lever media as a learning medium for VIII grade junior high school students on simple planes. This study uses the ADDIE development method which includes the analysis stage, the design stage, the development stage, the implementation stage, and the evaluation stage. The feasibility of the product developed in this study was obtained from the evaluation of the validation sheet by media expert validators and material experts, namely two science education lecturers at Hasyim Asy'ari University Tebuireng Jombang, and the practitioner validator, a science teacher from SMP Negeri 1 Jombang. From the assessment of the validation sheet obtained results in the form of qualitative and quantitative data. Qualitative data in the form of suggestions and input are used as a reference to improve and revise the lever media developed. While the quantitative data is processed to get the value of the validity of the developed lever media. Based on the results of the lever media validation assessment, it was obtained an average score of 3.82 in the very good category and a validity score of 95.5% in the very valid category.

Keywordsâ€‹: â€‹â€‹ Learning Media, Lever Media, Simple Plane
IMPLEMENTASI MODEL THINK TALK WRITE (TTW) DALAM PENINGKATAN HASIL BELAJAR SISWA MATERI PROCEDURE TEXT
Authors: Lies Muhsonati
Published: October 2023

The purposes of this study are to describe (1) the   implementation   of   the   Learning Implementation Plan during the learning process, (2) the learning outcomes of the product after the implementation of Think Talk Write, (3) student activities during the implementation of the Think Talk Write, and (4) students' responses to learning by applying the Think Talk Write learning model. This classroom action research was carried out for two cycles in class X MIPA 2 SMA Negeri 1 Puri involving 35 students. Each cycle consists of stages of planning, implementation, observation and reflection.  The results  showed  that  (1)  the  learning process by applying the Think Talk Write learning model could be carried out in good categories, namely in the first cycle with a value of 3.03 and the second cycle of 3.27., (2) student activities during the application of the Think Talk Write learning model showed students active with an activeness of 87.15% in the first cycle and 93.48% in the second cycle, (3) character behavior in both cycles showed good behavior with an average score of 3.23 (good) in the first cycle and 3.45 (good) in the second cycle, (4) results student learning after the implementation of the Think Talk Write learning model has increased with the percentage of completeness 77.14% in the first cycle increasing to 91.4% in the second cycle, and (5) student responses to learning by applying the Think Talk Write learning model are positive
Differentiation in Listening Activities: Doing What We Can with What We Have
Authors: Rodríguez-Clavero Raúl
Published: October 2023

This article summarizes the first action-research intervention conducted by a Chilean pre-service English as a Foreign Language (EFL) teacher in 2016. The main aim of this study was to measure the impact of implementing differentiated listening activities on the test results of a 7th grade classroom at a local private school. The class was divided into two groups based on their previous results of listening activities, and were assigned two different activities from their own coursebook. The lower proficiency learners were assigned a discrete-point testing task, whereas the higher achievers worked with an integrative testing activity. The two groups listened to the same audio while responding to different tasks. Test results were analyzed using quantitative methods. Results revealed that the use of differentiated activities had a significant impact on the listening tests scores of both groups when compared to their previous results. However, it could not be fully asserted if the changes on the results could be attributed only to the intervention applied. Finally, some reflections regarding the practicality of an intervention like this for a novice or pre-service EFL teacher needing to differentiate their listening activities will be shared.
Restaurant Liquid Waste Treatment into Clean Water Using Graded Filter-Ultrafiltration Membrane Polyethersulfone (PES) Method
Authors: Fadhilah Intan , Hamdan Abd Mujahid , Razi Fachrul
Published: October 2023

Restaurant liquid waste contains elevated pollutant levels and is commonly discharged directly into the environment, leading to adverse impacts on ecosystems and aquatic life. The traditional methods for processing such wastewater often involve extensive land usage and complicated operations, posing challenges for effective treatment. Therefore, there is a need for a more practical technology to manage restaurant liquid waste, and one promising approach is the utilization of membrane technology for wastewater treatment. In this study, the researchers employed a combination process of multilevel filters with downflow flow and PES membrane ultrafiltration to treat the restaurant liquid waste. The multilevel filter comprised several layers of filter media, including silica sand, activated carbon, zeolite, and gravel, with specific thicknesses on a PVC pipe. For the PES membrane, two different concentrations were tested: 15% and 20% PES. To evaluate the efficiency of the treatment process, pollutant parameters such as pH, TSS (Total Suspended Solids), COD (Chemical Oxygen Demand), and turbidity were measured. The results indicated significant pollutant reduction: pH levels could reach 6.9, TSS degradation achieved up to 98.37%, COD degradation up to 88.14%, and turbidity degradation up to 97.03%. Based on the outcomes, the most effective treatment for converting restaurant wastewater into clean water involved the combined use of multilevel filters and a 20% PES membrane ultrafiltration system.
Pengembangan Trainer Kit Berbasis Arduino Sebagai Media Pembelajaran Pada Mata Kuliah Robotika
Authors: Alamsyah Nur
Published: October 2023

Mata kuliah robotika merupakan salah satu mata kuliah wajib yang ada di Prodi Teknik Informatika Universitas Islam Makassar. Proses pembelajaran pada mata kuliah robotika berupa teori yang tidak diimplementasikan dengan alat peraga robotika, sehingga pemahaman pembelajaran robotika masih dikategorikan rendah. Tujuan penelitian menerapkan trainer kit berbasis arduino sebagai media pembelajara mata kuliah robotika guna memudahkan mahasiswa dan dosen dalam mengaplikasikan materi perkuliahan robotika. Metode penelitian Research and development (RD) dengan prosedur pengembangan mengacu pada model ADDIE yang bertujuan mengetahui unjuk kerja serta tingkat kelayakan trainer sebagai media pembelajaran. Hasil pengujian trainer ini terdiri dari beberapa komponen seperti arduino sebagai alat pengendali, sensor ultrasonik sebagai input pendeteksi jarak suatu objek, Motor DC sebagai output penggerak yang memiliki rotasi 3600, motor servo dengan rotasi putaran aksimal sebesar 1800 sebagai sendi penggerak dari robot arm LCD 16x2 sebagai output yang menampilkan teks, push button sebagai pengontrol suatu kondisi, joystik sebagai remot penggerak dari robot arm, dan Step down DC sebagai penyuplai tegangan dengan besaran 5 volt dan 12 volt, semua komponen dikemas dalam Box Aluminium dilengkapi dengan kabel USB yang berfungsi untuk mengirimkan program, serta adapter yang berfungsi sebagai power supply pada perangkat arduino uno. Hasil validasi uji coba pemakaian oleh dosen ahli memperoleh persentase sebesar 93,7% pada aspek edukatif, 87,5% pada aspek teknis, dan 85% pada aspek estetika. Kesimpulan media pembelajaran berupa trainet kit dapat dikategorikan sangat layak digunakan sebagai alat penunjang pembelajaran pada mata kuliah robotika teknik informatika universitas islam Makassar.
Perawatan Mesin SNS Cutter di PT.XYZ
Authors: Daniel Irfan
Published: October 2023

Penggunaan produk dari kaca di sektor industri mengalami peningkatan dikarenakan kaca banyak digunakan pada bangunan,  dan  automotive. Produksi kaca pada PT. XYZ memiliki sistem yang baik dari segi sumber daya  manusia  dan produk.  Kaca  yang  mengalami  kecacatan   juga dapat  diolah  kembali  sehingga  mengurangi kerugian  pada  produksi  dalam  perusahaan   kaca ini. Produksi yang baik juga disebabkan oleh mesin yang baik pula, sehingga mesin yang ada pada perusahaan ini harus di rawat  dengan  baik  agar tidak  terjadi  hambatan  proses  produksi   oleh karena  mesin  rusak  dan   lain   sebagainya. Penelitian ini berfokus  pada  mesin  sns  cutter karena mesin ini memiliki peran penting dalam pemotongan kaca untuk memenuhi kepuasaan konsumen  dalam  berbagai  ukuran.  Mesin   sns cutter ini memiliki macam-macam cara perawatan, memiliki berbagai spare part yang ada pada mesin sehingga harus dilakukan  pengecekan  secara berkala. Jenis-jenis perawatan yang ada pada mesin sns cutter, proses produksi dari kaca terbentuk sampai dipotong sesuai dengan keinginan konsumen, penyebab kerusakan mesin. Berdasarkan penelitian ini diketahui penyebab seringnya mesin mengalami kerusakan jika minyak tidak optimal sehingga pisau pada mesin cutter mengalami tumpul dan menyebabkan potongan kaca tidak maksimal. Perawatan pada mesin ini dilakukan dalam harian, mingguan, dan bulanan.
Efficiency of Monitoring Household Electricity With ZMCT103C
Authors: Suteja I Wayan Arsa , Antara Adi Surya , Wiantara Putra Gede Eka , Putu Ida Bagus
Published: October 2023

An electric load monitoring system is a tool designed to monitor and measure electricity usage. The process of monitoring the electrical load uses a current sensor model ZMCT103C which is connected to the electrical load. The microcontroller used is an Arduino Uno microcontroller which functions as the main control system which will be connected to a 16x2 LCD monitor with the aim that in the future it can be monitored in real time and can be read directly on the LCD. By carrying out experiments on assembling the ZMCT103C sensor module with Arduino Uno which produces a monitoring feature for current measurement readings. The result of this research is a household electricity measurement monitoring system through a prototype made using the ZMCT103C sensor model with an Arduino Uno which is equipped with a 16x2 LCD monitor to make it easier to monitor the electrical energy load from household electronic devices in the form of current readings. The magnitude of the current measurement obtained from the reading of the ZMCT103C model current sensor will be compared to the ampere meter measuring instrument. By conducting this research, we can obtain data on the percentage of error obtained from the application of the ZMCT103C sensor, so that later from the results of this experiment the ZMCT103C sensor will be able to properly and correctly monitor electricity usage for household loads.
Expanding structural diversity of 5′-aminouridine moiety of sansanmycin via mutational biosynthesis
Authors: Lu Yuan , Li Yihong , Fan Jiahui , Li Xingxing , Sun Hongmin , Wang Lifei , Han Xingli , Zhu Yuting , Zhang Tianyu , Shi Yuanyuan , Xie Yunying , Hong Bin
Published: October 2023

Sansanmycins represent a family of uridyl peptide antibiotics with antimicrobial activity specifically against Mycobacterium tuberculosis (including drug-resistant M. tuberculosis) and Pseudomonas aeruginosa. They target translocase I (MraY) to inhibit bacterial cell wall assembly. Given the unique mechanism of action, sansanmycin has emerged as a potential lead compound for developing new anti-tuberculosis drugs, while the 5′-aminouridine moiety plays a crucial role in the pharmacophore of sansanmycin. For expanding the structural diversity of the 5′-aminouridine moiety of sansanmycin through biosynthetic methods, we firstly demonstrated that SsaM and SsaK are responsible for the biosynthesis of the 5′-aminouridine moiety of sansanmycin in vivo. Using the ssaK deletion mutant (SS/KKO), we efficiently obtained a series of new analogues with modified 5′-aminouridine moieties through mutational biosynthesis. Based on molecular networking analysis of MS/MS, twenty-two new analogues (SS-KK-1 to -13 and SS-KK-A to -I) were identified. Among them, four new analogues (SS-KK-1 to -3 and SS-KK-C) were purified and bioassayed. SS-KK-2 showed better antibacterial activity against E. coli ΔtolC than the parent compound sansanmycin A. SS-KK-3 showed the same anti-TB activity as sansanmycin A against M. tuberculosis H37Rv as well as clinically isolated, drug-sensitive and multidrug-resistant M. tuberculosis strains. Furthermore, SS-KK-3 exhibited significantly improved structural stability compared to sansanmycin A. The results suggested that mutasynthesis is an effective and practical strategy for expanding the structural diversity of 5′-aminouridine moiety in sansanmycin.
Exploring stimuli-responsive elastin-like polypeptide for biomedicine and beyond: potential application as programmable soft actuators
Authors: Noh Yeongjin , Son Eunjoo , Cha Chaenyung
Published: October 2023

With the emergence of soft robotics, there is a growing need to develop actuator systems that are lightweight, mechanically compliant, stimuli-responsive, and readily programmable for precise and intelligent operation. Therefore, “smart” polymeric materials that can precisely change their physicomechanical properties in response to various external stimuli (e.g., pH, temperature, electromagnetic force) are increasingly investigated. Many different types of polymers demonstrating stimuli-responsiveness and shape memory effect have been developed over the years, but their focus has been mostly placed on controlling their mechanical properties. In order to impart complexity in actuation systems, there is a concerted effort to implement additional desired functionalities. For this purpose, elastin-like polypeptide (ELP), a class of genetically-engineered thermoresponsive polypeptides that have been mostly utilized for biomedical applications, is being increasingly investigated for stimuli-responsive actuation. Herein, unique characteristics and biomedical applications of ELP, and recent progress on utilizing ELP for programmable actuation are introduced.
Novel role for alpha-2-macroglobulin (A2M) as a disease modifying protein in senile osteoporosis
Authors: Boregowda Siddaraju V. , Haga Christopher L. , Supper Valentina M. , Booker Cori N. , Phinney Donald G.
Published: October 2023

Introduction: In the rapidly aging U.S. population, age-induced bone loss (senile osteoporosis) represents a major public health concern that is associated with a significant increased risk for low trauma fragility fractures, which are debilitating to patients, cause significant morbidity and mortality, and are costly to treat and manage. While various treatments exist to slow bone loss in osteoporosis patients, these suffer from poor tolerability and label restrictions that limit their overall effectiveness. Over the past decade, skeletal stem/progenitor cells (SSPCs), which are the main precursor of osteoblasts and adipocytes in adult bone marrow (BM), have emerged as important players in osteoporosis.

Methods: Age-induced skeletal pathology was quantified in elderly (24-month-old) vs. mature (3-month-old) mice by micro-CT and changes in SSPC abundance in the BM of these mice was quantified by fluorescence-activated cell sorting (FACS). SSPCs from elderly vs. mature mice were also analyzed by RNA-Seq to identify differentially expressed genes (DEGs), and gain and loss-of-function studies were performed in human BM-derived mesenchymal stromal cells (BM-MSCs) to assess A2M function.

Results: Elderly mice were shown to exhibit significant age-induced skeletal pathology, which correlated with a significant increase in SSPC abundance in BM. RNA-seq analysis identified alpha-2-macroglobulin (A2M), a pan-protease inhibitor that also binds inflammatory cytokines, as one of the most downregulated transcripts in SSPCs isolated from the BM of elderly vs. mature mice, and silencing of A2M expression in human BM-MSCs induced their proliferation and skewed their lineage bifurcation toward adipogenesis at the expense of osteogenesis thereby recapitulating critical aspects of age-induced stem cell dysfunction.

Conclusion: These findings identify A2M as a novel disease modifying protein in osteoporosis, downregulation of which in bone marrow promotes SSPC dysfunction and imbalances in skeletal homeostasis.
Rapid diagnosis of Mycobacterium marinum infection using targeted nanopore sequencing: a case report
Authors: Huang Yan-Ying , Li Qiu-Shi , Li Zhao-Dong , Sun Ai-Hua , Hu Sheng-Ping
Published: October 2023

Mycobacterium marinum (M. marinum) is a non-tuberculous mycobacterium (NTM) that can cause infectious diseases in aquatic animals and humans. Culture-based pathogen detection is the gold standard for diagnosing NTM infection. However, this method is time-consuming and has low positivity rates for fastidious organisms. Oxford Nanopore MinION sequencing is an emerging third-generation sequencing technology that can sequence DNA or RNA directly in a culture-independent manner and offers rapid microbial identification. Further benefits include low cost, short turnaround time, long read lengths, and small equipment size. Nanopore sequencing plays a crucial role in assessing drug resistance, clinical identification of microbes, and monitoring infectious diseases. Some reports on Mycobacterium tuberculosis (MTB) using nanopore sequencing have been published, however, there are few reports on NTM, such as M. marinum. Here, we report the use of nanopore sequencing for the diagnosis of M. marinum.
Drug-resistant strains of Mycobacterium tuberculosis: cell envelope profiles and interactions with the host
Authors: Schami Alyssa , Islam M. Nurul , Belisle John T. , Torrelles Jordi B.
Published: October 2023

In the past few decades, drug-resistant (DR) strains of Mycobacterium tuberculosis (M.tb), the causative agent of tuberculosis (TB), have become increasingly prevalent and pose a threat to worldwide public health. These strains range from multi (MDR) to extensively (XDR) drug-resistant, making them very difficult to treat. Further, the current and future impact of the Coronavirus Disease 2019 (COVID-19) pandemic on the development of DR-TB is still unknown. Although exhaustive studies have been conducted depicting the uniqueness of the M.tb cell envelope, little is known about how its composition changes in relation to drug resistance acquisition. This knowledge is critical to understanding the capacity of DR-M.tb strains to resist anti-TB drugs, and to inform us on the future design of anti-TB drugs to combat these difficult-to-treat strains. In this review, we discuss the complexities of the M.tb cell envelope along with recent studies investigating how M.tb structurally and biochemically changes in relation to drug resistance. Further, we will describe what is currently known about the influence of M.tb drug resistance on infection outcomes, focusing on its impact on fitness, persister-bacteria, and subclinical TB.
Comparative analysis of clinical and immunological profiles across Omicron BA.5.2 subvariants using next-generation sequencing in a Chinese cohort
Authors: Huang Jianliang , Xia Mingkai , Liu Rangjiao , Wang Shaobo , Duan Xinyi , Peng Jiong , Li Enping , Zhou Yanping , Li Chengyou , Zhang Quan , Tian Jixian , Wang Xinjian , Su Zhongrui , Tan Jun , Peng Bo , Zhang Jianhui , Li Jin , Dai Lizhong , Lei Mingsheng
Published: October 2023

Objective

The Omicron BA.5.2 variant of SARS-CoV-2 has undergone several evolutionary adaptations, leading to multiple subvariants. Rapid and accurate characterization of these subvariants is essential for effective treatment, particularly in critically ill patients. This study leverages Next-Generation Sequencing (NGS) to elucidate the clinical and immunological features across different Omicron BA.5.2 subvariants.

Methods

We enrolled 28 patients infected with the Omicron variant, hospitalized in Zhangjiajie People’s Hospital, Hunan, China, between January 20, 2023, and March 31, 2023. Throat swabs were collected upon admission for NGS-based identification of Omicron subvariants. Clinical data, including qSOFA scores and key laboratory tests, were collated. A detailed analysis of lymphocyte subsets was conducted to ascertain the extent of immune cell damage and disease severity.

Results

Patients were infected with various Omicron subvariants, including BA.5.2.48, BA.5.2.49, BA.5.2.6, BF.7.14, DY.1, DY.2, DY.3, and DY.4. Despite having 43 identical mutation sites, each subvariant exhibited unique marker mutations. Critically ill patients demonstrated significant depletion in total lymphocyte count, T cells, CD4, CD8, B cells, and NK cells (P < 0.05). However, there were no significant differences in clinical and immunological markers across the subvariants.

Conclusion

This study reveals that critically ill patients infected with different Omicron BA.5.2 subvariants experience similar levels of cellular immune dysfunction and inflammatory response. Four mutations - ORF1a:K3353R, ORF1a:L3667F, ORF1b:S997P, S:T883I showed correlation with immunological responses although this conclusion suffers from the small sample size. Our findings underscore the utility of NGS in the comprehensive assessment of infectious diseases, contributing to more effective clinical decision-making.
“Talk to the hand”: handling peer conflict through gestural socialization in an elementary classroom
Authors: Corella Meghan
Published: October 2023

Although researchers of language and communication have become increasingly interested in both embodiment and conflict in recent years, little is known about how elementary students use embodied actions modeled by their teachers as they engage in peer conflicts. This paper addresses such questions, focusing on the “quiet coyote” gesture and the “open hand prone” gesture, two emblems commonly used as classroom management strategies in elementary grades. Building on work in language socialization, gesture studies, and other areas of discourse analysis, I propose what I call a gestural socialization perspective for analyzing the nuanced ways the US second-grade children in this study use and socialize one another to use these gestures, as well as other semiotic resources, to handle peer disputes. An ethnographically informed, multimodal discourse analysis centering on a multiracial group of girls reveals how students' gesture practices draw on their teacher's gestural socialization practices while also diverging from them, especially with regard to gestural form, stance object, intended recipient, and accompanying metapragmatic commentary. These aspects of the participants' appropriations of the “open hand prone” and “quiet coyote” emblems, together with their use of gestural innovations, metagestures, and other semiotic resources, allow them to take more oppositional stances than those made relevant by the teacher's practices. Through these multimodal stances, students take a hands-on approach to starting, continuing, and closing peer disputes on their own terms. Overall, the study highlights how participants' handling of disputes often subverted a local emphasis on conflict avoidance, efficiency, and appropriateness and the developmentalist, neoliberal, and standard language ideologies underpinning these norms. The paper closes with a discussion of implications for research and pedagogy, emphasizing the importance of closely attending to the multimodal, interactionally emergent, and culturally situated nature of conflicts among children and people of all ages.
Symptomatic periesophageal vagal nerve injury by different energy sources during atrial fibrillation ablation
Authors: Miyazaki Shinsuke , Kobori Atsushi , Jo Hikari , Keida Takehiko , Yoshitani Kazuyasu , Mukai Moe , Sagawa Yuichiro , Asakawa Tetsuya , Sato Eiji , Yamao Kazuya , Horie Tomoki , Manita Mamoru , Fukaya Hidehira , Hayashi Hidemori , Tanimoto Kojiro , Iwayama Tadateru , Chiba Suguru , Sato Akinori , Sekiguchi Yukio , Sugiura Kenta , Iwai Shinsuke , Isonaga Yuhei , Miwa Naoyuki , Kato Nobutaka , Inaba Osamu , Hirota Takayoshi , Nagata Yasutoshi , Ono Yuichi , Hachiya Hitoshi , Yamauchi Yasuteru , Goya Masahiko , Nitta Junichi , Tada Hiroshi , Sasano Tetsuo
Published: October 2023

Background

Symptomatic gastric hypomotility (SGH) is a rare but major complication of atrial fibrillation (AF) ablation, but data on this are scarce.

Objective

We compared the clinical course of SGH occurring with different energy sources.

Methods

This multicenter study retrospectively collected the characteristics and clinical outcomes of patients with SGH after AF ablation.

Results

The data of 93 patients (67.0 ± 11.2 years, 68 men, 52 paroxysmal AF) with SGH after AF ablation were collected from 23 cardiovascular centers. Left atrial (LA) ablation sets included pulmonary vein isolation (PVI) alone, a PVI plus a roof-line, and an LA posterior wall isolation in 42 (45.2%), 11 (11.8%), and 40 (43.0%) patients, respectively. LA ablation was performed by radiofrequency ablation, cryoballoon ablation, or both in 38 (40.8%), 38 (40.8%), and 17 (18.3%) patients, respectively. SGH diagnoses were confirmed at 2 (1–4) days post-procedure, and 28 (30.1%) patients required re-hospitalizations. Fasting was required in 81 (92.0%) patients for 4 (2.5–5) days; the total hospitalization duration was 11 [7–19.8] days. After conservative treatment, symptoms disappeared in 22.3% of patients at 1 month, 48.9% at 2 months, 57.6% at 3 months, 84.6% at 6 months, and 89.7% at 12 months, however, one patient required surgery after radiofrequency ablation. Symptoms persisted for >1-year post-procedure in 7 patients. The outcomes were similar regardless of the energy source and LA lesion set.

Conclusions

The clinical course of SGH was similar regardless of the energy source. The diagnosis was often delayed, and most recovered within 6 months, yet could persist for over 1 year in 10%.
Plant communities and food webs
Authors: Dobson Andy , Hutchinson Matthew C. , Batterman Sarah
Published: October 2023

Recent theoretical work has provided major new insights into the ways that species interactions in food webs are organized in ways that permit the coexistence of significant numbers of species. But, we seem to have forgotten about trees! Not the phylogenetic ones that are increasingly important for dissecting the evolutionary structure of food webs, but the trees, shrubs and grasses that are the basal species in all terrestrial ecosystems. Many of the food webs available for analysis over the last 30 years were based on freshwater or marine systems where algae were the main plants. Trees are very different from algae; they can live for centuries, while annually producing leaves, fruits and seeds that provide nutrients for a diversity of species on higher trophic levels. In sharp contrast to algae, they are only partly consumed by herbivores and usually compensate or recover from herbivory. Most of the biomass in terrestrial systems is in the plants, this again contrasts with aquatic systems, where most of the biomass is in primary and secondary consumers. Moreover, each individual tree supports its own food web of species that are only partially coupled to those of surrounding trees. If we are going to apply our theoretical understanding of food-web structure to species-rich terrestrial ecosystems in ways that are insightful for conservation, then we need a deeper examination of the role that higher plants play in food webs. While community ecology has developed an increasingly detailed understanding of the ways plant communities are organized, this seems to have evolved almost independently of the food-web literature. In this article, we make a plea to more sharply consider higher plants in food webs and to do this by combining recent theoretical work on food webs, with recent empirical and theoretical work on plant communities. Ultimately, we argue for a deeper integration of plant community ecology into studies of food webs.