bluuebunny/crossref_metadata_2025 huggingface dataset:
Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog
Authors: Chandrasekaran Abinaya , Thomsen Barbara Blicher , Agerholm Jørgen Steen , Pessôa Laís Vicari de Figueiredo , Godoy Pieri Naira Caroline , Sabaghidarmiyan Vahideh , Langley Katarina , Kolko Miriam , de Andrade André Furugen Cesar , Bressan Fabiana Fernandes , Hyttel Poul , Berendt Mette , Freude Kristine
Published: November 2021

Domestic dogs are superior models for translational medicine due to greater anatomical and physiological similarities with humans than rodents, including hereditary diseases with human equivalents. Particularly with respect to neurodegenerative medicine, dogs can serve as a natural, more relevant model of human disease compared to transgenic rodents. Herein we report attempts to develop a canine-derived in vitro model for neurodegenerative diseases through the generation of induced pluripotent stem cells from a 14-year, 9-month-old female West Highland white terrier with mild cognitive impairment (MCI). Canine induced pluripotent stem cells-like cells (ciPSCLC) were generated using human OSKM and characterized by positive expression of pluripotency markers. Due to inefficient viral vector silencing we refer to them as ciPSCLCs. Subsequently, the ciPSCLC were subjected to neural induction according to two protocols both yielding canine neural progenitor cells (cNPCs), which expressed typical NPC markers. The cNPCs were cultured in neuron differentiation media for 3 weeks, resulting in the derivation of morphologically impaired neurons as compared to iPSC-derived human counterparts generated in parallel. The apparent differences encountered in this study regarding the neural differentiation potential of ciPSCLC reveals challenges and new perspectives to consider before using the canine model in translational neurological studies.
Modelling of Microperforated Panel Absorbers with Circular and Slit Hole Geometries
Authors: Cobo Pedro
Published: November 2021

Although the original proposal of microperforated panels by Maa consisted of an array of minute circular holes evenly distributed in a thin plate, other hole geometries have been recently suggested that provide similar absorption curves to those of circular holes. With the arrival of modern machining technologies, such as 3D printing, panels microperforated with slit-shaped holes are being specially considered. Therefore, models able to predict the absorption performance of microperforated panels with variable hole geometry are needed. The aim of this article is to analyze three models for such absorbing systems, namely, the Maa model for circular holes, the Randeberg–Vigran model for slit-shaped holes, and the Equivalent Fluid model for both geometries. The absorption curves predicted for these three models are compared with the measured curves of three panels microperforated with spirally shaped slits.
Effects of Agricultural Use on Endangered Plant Taxa in Spain
Authors: Molina-Pardo José Luis , Rodríguez-Caballero Emilio , Cueto Miguel , Barranco Pablo , Sánchez-Robles Manuel , Laguía-Allué Azucena , Giménez-Luque Esther
Published: November 2021

Agriculture is one of the most widespread human activities and has the greatest impact on terrestrial ecosystems, as it transforms natural ecosystems into artificial landscapes using, in many cases, large amounts of pesticides as well as overexploiting natural resources. Therefore, for effective biodiversity conservation, it is necessary to include agricultural systems in conservation programs. In this work, the 50 plant taxa described for Spain as threatened by agricultural use were selected. These were divided according to the type of threat into those affected by crop extension, intensification, or abandonment. In addition, information was obtained concerning their conservation status, level of protection and functional traits (life form, pollination, and dispersal). Finally, the evolution of land use, in the areas near the populations of the selected species, was identified. The selected taxa belong to 21 families and present different life forms and modes of dispersal or pollination. Forty-six percent are endangered (EN) and most are included in legal protection lists. Nearly three-quarters are threatened by crop expansion and land use dynamics, reflecting an expansion of cultivated areas, which adds further pressure to these species. In addition to agricultural expansion, taxa are also at risk, due to important rates of agricultural land abandonment, and mention agricultural intensification. Nevertheless, conservation measures do exist to promote biodiversity in agricultural landscapes that may help to reverse the negative effect of land use dynamics on selected species, but few are specific to threatened flora. Therefore, if threatened plants are to be conserved in agricultural areas, it is necessary to promote a profound transformation of our socioecological systems. One of these transformative changes could come from the human-nature reconnection.
Fusarium Secondary Metabolite Content in Naturally Produced and Artificially Provoked FHB Pressure in Winter Wheat
Authors: Sunic Katarina , Kovac Tihomir , Loncaric Ante , Babic Jurislav , Sulyok Michael , Krska Rudolf , Drezner Georg , Spanic Valentina
Published: November 2021

Fusarium head blight (FHB) is an important disease of wheat and production of mycotoxins makes it a major threat in most wheat-producing areas worldwide. This study aimed to identify the impact of epidemic FHB conditions (usage of artificial Fusarium inoculation) on mycotoxin levels in unprocessed wheat. Fusarium levels were monitored at two locations in two treatments (natural infection and inoculation with Fusarium graminearum and F. culmorum) where 13 mycotoxins were evaluated by LC/MS-MS in six winter wheat varieties. Due to favorable conditions for infection with Fusarium fungi during the flowering period at location Tovarnik, wheat varieties had higher disease severity and increased mycotoxin accumulation, compared to Osijek. The most abundant mycotoxins in treatment with inoculation with Fusarium fungi were deoxynivalenol (DON), culmorin (CUL) and hydroxyculmorins. In treatment with natural infection, DON did not exceed maximum limits set by EU. Varieties with lower initial resistance accumulated DON even in naturally infected samples at Tovarnik. These results highlighted the impact of environment variation in the production of Fusarium mycotoxins where FHB initial resistance had a higher impact on the accumulation of mycotoxins than general resistance. Furthermore, wheat samples with higher DON concentration also contained elevated levels of CUL and hydroxyculmorins, showing that CUL can have a possible role in Fusarium virulence. The FHB evaluations provide important information about the genetic resistance of wheat varieties, as well as risk assessment considering mycotoxin accumulation in epidemic conditions.
Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
Authors: Jasiński Tomasz , Zdrojkowski Łukasz , Kautz Ewa , Juszczuk-Kubiak Edyta , Ferreira-Dias Graça , Domino Małgorzata
Published: November 2021

Endometrosis is an important mares’ disease which considerably decreases their fertility. As classic endometrial classification methods might be insufficient for tissue pathological evaluation, further categorization into active/inactive and destructive/non-destructive types was developed by Hoffmann and others. This study aimed to compare NF-κB pathway genes transcription among histopathological types of endometrosis, following Hoffmann and co-authors’ classification. Endometrial samples, collected postmortem from cyclic mares (n = 100) in estrus or diestrus, were classified histologically and used for gene transcription assessment. Gene transcription of NF-κB subunits (RelA, NF-κB1, NF-κB2), pro-inflammatory molecules (MCP-1, IL-6), and hyaluronan synthases (HAS 1, HAS 2, HAS 3) was compared among endometrosis types (active, non-active, destructive, non-destructive). Most individual mRNA samples showed high expression of RelA, NF-κB1, and MCP-1 gene transcripts and the destructive type of endometrosis, simultaneously. The expression of RelA and NF-κB1 genes was higher in active destructive group than in the other groups only in the follicular phase, as well as being higher in the inactive destructive group than in the others, only in the mid-luteal phase. The increase in gene transcription of the NF-κB canonical activation pathway in destructive endometrosis may suggest the highest changes in extracellular matrix deposition. Moreover, the estrous cycle phase might influence fibrosis pathogenesis.
Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
Authors: Fu Lin , Zhang Li , Liu Li , Yang Heng , Zhou Peng , Song Fan , Dong Guozhong , Chen Juncai , Wang Gaofu , Dong Xianwen
Published: November 2021

Heat stress (HS) is one of the most serious factors to negatively affect the lactation performance of dairy cows. Bovine mammary epithelial cells are important for lactation. It was demonstrated that HS decreases the lactation performance of dairy cows, partly through altering gene expression within bovine mammary epithelial tissue. However, the cellular metabolism mechanisms under HS remains largely unknown. The objective of this study was to determine whether HS induced changes in intracellular metabolites and gene transcription related to amino acid metabolism, amino acid transportation and the mTOR signaling pathway. Immortalized bovine mammary epithelial cell lines (MAC-T cells, n = 5 replicates/treatment) were incubated for 12 h at 37 °C (Control group) and 42 °C (HS group). Relative to the control group, HS led to a greater mRNA expression of heat shock protein genes HSF1, HSPB8, HSPA5, HSP90AB1 and HSPA1A. Compared with the control group, metabolomics using liquid chromatography tandem–mass spectrometry identified 417 differential metabolites with p < 0.05 and a variable importance in projection (VIP) score >1.0 in the HS group. HS resulted in significant changes to the intracellular amino acid metabolism of glutathione, phenylalanine, tyrosine, tryptophan, valine, leucine, isoleucine, arginine, proline, cysteine, methionine, alanine, aspartate and glutamate. HS led to a greater mRNA expression of the amino acid transporter genes SLC43A1, SLC38A9, SLC36A1, and SLC3A2 but a lower mRNA expression of SLC7A5 and SLC38A2. Additionally, HS influenced the expression of genes associated with the mTOR signaling pathway and significantly upregulated the mRNA expression of mTOR, AKT, RHEB, eIF4E and eEF2K but decreased the mRNA expression of TSC1, TSC2 and eEF2 relative to the control group. Compared with the control group, HS also led to greater mRNA expression of the CSN1S2 gene. Overall, our study indicates that bovine mammary epithelial cells may have the ability to resist HS damage and continue milk protein synthesis partly through enhanced intracellular amino acid absorption and metabolism and by activating the mTOR signaling pathway during HS.
Effects of Clostridium butyricum and a Bacteriophage Cocktail on Growth Performance, Serum Biochemistry, Digestive Enzyme Activities, Intestinal Morphology, Immune Responses, and the Intestinal Microbiota in Rabbits
Authors: Huang Pan , Cui Xuemei , Wang Zhipeng , Xiao Chenwen , Ji Quanan , Wei Qiang , Huang Yee , Bao Guolian , Liu Yan
Published: November 2021

The objective of this study was to assess the effects of dietary supplementation with Clostridium butyricum (CB) and a bacteriophage cocktail (BP) on growth performance, serum biochemical parameters, intestinal digestive and oxidase enzymes, intestinal morphology, immune responses, and the cecum microbiota in rabbits. In total, 108 New Zealand rabbits (5 weeks old) were randomly and equally allotted into three dietary treatment groups (four replicates per treatment, n = 36/treatment): (1) the control (CN) group—rabbits fed the basal diet; (2) CB group—rabbits fed the basal diet supplemented with 100 mg/kg diet Clostridium butyricum; and (3) BP group—rabbits fed the basal diet supplemented with 200 mg/kg diet BP cocktail, respectively, for 6 weeks. Compared with the CN diet, dietary CB and BP inclusion increased the average daily gain (ADG) and average daily feed intake (ADFI) and decreased the feed/gain (F/G) ratio of rabbits. Furthermore, CB increased the digestive enzyme activity (α-amylase and trypsin in the ileum); the chymotrypsin activity was also significantly increased in the duodenum and jejunum. Supplementation with CB significantly enhanced antioxidant capacity (SOD and GSH-Px) in the jejunum and ileum and reduced MDA levels. Additionally, rabbits fed CB had significantly elevated villus height (V) and (V/C) ratios but reduced crypt depth (C). Moreover, dietary CB supplementation markedly increased the ileal expression of tight junction proteins (occludin, ZO-1, and claudin-1) and increased secretory immunoglobulin A (sIgA) production. High-throughput sequencing indicated that the microbiota in the rabbit intestine was altered by CB and BP. Venn diagrams and heatmap plots revealed that the gut microbial community composition varied obviously among rabbits fed different diets. Specifically, CB increased the relative abundance of beneficial bacteria to maintain intestinal barrier homeostasis, whereas BP decreased the relative abundance of Gammaproteobacteria, which included a plenty of pathogenic bacteria.
Presynaptic Release-Regulating Alpha2 Autoreceptors: Potential Molecular Target for Ellagic Acid Nutraceutical Properties
Authors: Romeo Isabella , Vallarino Giulia , Turrini Federica , Roggeri Alessandra , Olivero Guendalina , Boggia Raffaella , Alcaro Stefano , Costa Giosuè , Pittaluga Anna
Published: November 2021

Polyphenol ellagic acid (EA) possesses antioxidant, anti-inflammatory, anti-carcinogenic, anti-diabetic and cardio protection activities, making it an interesting multi-targeting profile. EA also controls the central nervous system (CNS), since it was proven to reduce the immobility time of mice in both the forced swimming and the tail-suspension tests, with an efficiency comparable to that of classic antidepressants. Interestingly, the anti-depressant-like effect was almost nulled by the concomitant administration of selective antagonists of the noradrenergic receptors, suggesting the involvement of these cellular targets in the central effects elicited by EA and its derivatives. By in silico and in vitro studies, we discuss how EA engages with human α2A-ARs and α2C-AR catalytic pockets, comparing EA behaviour with that of known agonists and antagonists. Structurally, the hydrophobic residues surrounding the α2A-AR pocket confer specificity on the intermolecular interactions and hence lead to favourable binding of EA in the α2A-AR, with respect to α2C-AR. Moreover, EA seems to better accommodate within α2A-ARs into the TM5 area, close to S200 and S204, which play a crucial role for activation of aminergic GPCRs such as the α2-AR, highlighting its promising role as a partial agonist. Consistently, EA mimics clonidine in inhibiting noradrenaline exocytosis from hippocampal nerve endings in a yohimbine-sensitive fashion that confirms the engagement of naïve α2-ARs in the EA-mediated effect.
Development Length of Fluids Modelled by the gPTT Constitutive Differential Equation
Authors: Bertoco Juliana , Leiva Rosalía T. , Ferrás Luís L. , Afonso Alexandre M. , Castelo Antonio
Published: November 2021

In this work, we present a numerical study on the development length (the length from the channel inlet required for the velocity to reach 99% of its fully-developed value) of a pressure-driven viscoelastic fluid flow (between parallel plates) modelled by the generalised Phan–Thien and Tanner (gPTT) constitutive equation. The governing equations are solved using the finite-difference method, and, a thorough analysis on the effect of the model parameters α and β is presented. The numerical results showed that in the creeping flow limit (Re=0), the development length for the velocity exhibits a non-monotonic behaviour. The development length increases with Wi. For low values of Wi, the highest value of the development length is obtained for α=β=0.5; for high values of Wi, the highest value of the development length is obtained for α=β=1.5. This work also considers the influence of the elasticity number.
State of the Art in Metal Matrix Composites Research: A Bibliometric Analysis
Authors: Sekhar Ravi , Sharma Deepak , Shah Pritesh
Published: November 2021

Metal matrix composites (MMC) are the materials of tomorrow. This paper presents an in-depth analysis of the MMC research articles published in Web of Science (WoS) during 2001–2020. The study firstly included year on year publications, publication types, sources, research directions as well as the most productive researchers, organizations and nations. Secondly, a detailed analysis of collaborations among various MMC researchers, organizations and countries has been presented. Thirdly, citations based linkages among the published articles, sources, researchers, institutions and places have been discussed relative to their respective collaborative link strengths. A co-occurrence analysis of MMC keywords was also conducted to highlight the most important keywords trending in this area. Finally, burst detection analyses of keywords and references were carried out to unearth sudden citation spikes of keywords and documents. Primary results indicate that research articles formed 80.54% of all MMC publications in WoS. The journal ‘Materials Science and Engineering A: Structural Materials, Properties, Microstructure and Processing’ published maximum MMC articles. Collaboration analysis results indicate that Zhang D, the Chinese Academy of Science and People’s Republic of China, attained topmost collaboration based total link strengths (TLS). Citations based analysis showed that Zhang D, the Shanghai Jiao Tong University (China), People’s Republic of China and the journal ‘Materials Science and Engineering A: Structural Materials, Properties, Microstructure and Processing’ received highest citation TLS values. Keyword ‘Graphene’ scored the highest citation burst strength (2018–2020). The future of MMC research lies in processing and characterization of novel nanocomposites with reinforcements such as graphene and boron carbide for various applications.
How Does Learning Analytics Contribute to Prevent Students’ Dropout in Higher Education: A Systematic Literature Review
Authors: de Oliveira Catarina Félix , Sobral Sónia Rolland , Ferreira Maria João , Moreira Fernando
Published: November 2021

Retention and dropout of higher education students is a subject that must be analysed carefully. Learning analytics can be used to help prevent failure cases. The purpose of this paper is to analyse the scientific production in this area in higher education in journals indexed in Clarivate Analytics’ Web of Science and Elsevier’s Scopus. We use a bibliometric and systematic study to obtain deep knowledge of the referred scientific production. The information gathered allows us to perceive where, how, and in what ways learning analytics has been used in the latest years. By analysing studies performed all over the world, we identify what kinds of data and techniques are used to approach the subject. We propose a feature classification into several categories and subcategories, regarding student and external features. Student features can be seen as personal or academic data, while external factors include information about the university, environment, and support offered to the students. To approach the problems, authors successfully use data mining applied to the identified educational data. We also identify some other concerns, such as privacy issues, that need to be considered in the studies.
Patient-Specific Analysis of Ascending Thoracic Aortic Aneurysm with the Living Heart Human Model
Authors: Cutugno Salvatore , Agnese Valentina , Gentile Giovanni , Raffa Giuseppe M. , Wisneski Andrew D. , Guccione Julius M. , Pilato Michele , Pasta Salvatore
Published: November 2021

In ascending thoracic aortic aneurysms (ATAAs), aneurysm kinematics are driven by ventricular traction occurring every heartbeat, increasing the stress level of dilated aortic wall. Aortic elongation due to heart motion and aortic length are emerging as potential indicators of adverse events in ATAAs; however, simulation of ATAA that takes into account the cardiac mechanics is technically challenging. The objective of this study was to adapt the realistic Living Heart Human Model (LHHM) to the anatomy and physiology of a patient with ATAA to assess the role of cardiac motion on aortic wall stress distribution. Patient-specific segmentation and material parameter estimation were done using preoperative computed tomography angiography (CTA) and ex vivo biaxial testing of the harvested tissue collected during surgery. The lumped-parameter model of systemic circulation implemented in the LHHM was refined using clinical and echocardiographic data. The results showed that the longitudinal stress was highest in the major curvature of the aneurysm, with specific aortic quadrants having stress levels change from tensile to compressive in a transmural direction. This study revealed the key role of heart motion that stretches the aortic root and increases ATAA wall tension. The ATAA LHHM is a realistic cardiovascular platform where patient-specific information can be easily integrated to assess the aneurysm biomechanics and potentially support the clinical management of patients with ATAAs.
Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions
Authors: Tamburrini Ketty C. , Terrapon Nicolas , Lombard Vincent , Bissaro Bastien , Longhi Sonia , Berrin Jean-Guy
Published: November 2021

Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses. LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9–11, AA13–17) within the Auxiliary Activity enzyme class of the CAZy database. LPMOs minimal architecture encompasses a catalytic domain, to which can be appended a carbohydrate-binding module. Intriguingly, we observed that some LPMO sequences also display a C-terminal extension of varying length not associated with any known function or fold. Here, we analyzed 27,060 sequences from different LPMO families and show that 60% have a C-terminal extension predicted to be intrinsically disordered. Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition. Noteworthily, these dCTRs have so far only been observed in LPMOs. LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications. Interestingly, dCTRs from AA11 and AA15 are enriched in redox-sensitive, conditionally disordered regions. The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
An Epigenetic Insight into NLRP3 Inflammasome Activation in Inflammation-Related Processes
Authors: Raneros Aroa Baragaño , Bernet Cristian Ruiz , Flórez Aida Bernardo , Suarez-Alvarez Beatriz
Published: November 2021

Aberrant NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) inflammasome activation in innate immune cells, triggered by diverse cellular danger signals, leads to the production of inflammatory cytokines (IL-1β and IL-18) and cell death by pyroptosis. These processes are involved in the pathogenesis of a wide range of diseases such as autoimmune, neurodegenerative, renal, metabolic, vascular diseases and cancer, and during physiological processes such as aging. Epigenetic dynamics mediated by changes in DNA methylation patterns, chromatin assembly and non-coding RNA expression are key regulators of the expression of inflammasome components and its further activation. Here, we review the role of the epigenome in the expression, assembly, and activation of the NLRP3 inflammasome, providing a critical overview of its involvement in the disease and discussing how targeting these mechanisms by epigenetic treatments could be a useful strategy for controlling NLRP3-related inflammatory diseases.
Microbiota: An Emerging Biomarker in Colorectal Cancer
Authors: Tsiaoussis John , Souglakos John
Published: November 2021

This series of 10 articles (four original articles, five literature reviews, and one systematic review) is presented by international experts in the study of microbiota and its relation to colorectal cancer (CRC) [...]
Epithelial to Mesenchymal Transition: Key Regulator of Pancreatic Ductal Adenocarcinoma Progression and Chemoresistance
Authors: Palamaris Kostas , Felekouras Evangelos , Sakellariou Stratigoula
Published: November 2021

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest malignancies, characterized by aggressive biological behavior and a lack of response to currently available chemotherapy. Emerging evidence has identified epithelial to mesenchymal transition (EMT) as a key driver of PDAC progression and a central regulator in the development of drug resistance. EMT is a reversible transdifferentiation process controlled by complex interactions between multiple signaling pathways such as TGFb, Wnt, and Notch, which converge to a network of specific transcription factors. Activation of EMT transcriptional reprogramming converts cancer cells of epithelial differentiation into a more mesenchymal phenotypic state. EMT occurrence in pre-invasive pancreatic lesions has been implicated in early PDAC dissemination. Moreover, cancer cell phenotypic plasticity driven by EMT contributes to intratumoral heterogeneity and drug tolerance and is mechanistically associated with the emergence of cells exhibiting cancer stem cells (CSCs) phenotype. In this review we summarize the available data on the signaling cascades regulating EMT and the molecular isnteractions between pancreatic cancer and stromal cells that activate them. In addition, we provide a link between EMT, tumor progression, and chemoresistance in PDAC.
BTLA Expression in CLL: Epigenetic Regulation and Impact on CLL B Cell Proliferation and Ability to IL-4 Production
Authors: Karabon Lidia , Andrzejczak Anna , Ciszak Lidia , Tomkiewicz Anna , Szteblich Aleksandra , Bojarska-Junak Agnieszka , Roliński Jacek , Wołowiec Dariusz , Wróbel Tomasz , Kosmaczewska Agata
Published: November 2021

In our previous study, while chronic lymphocytic leukemia (CLL) cases showed higher levels of B and T lymphocyte attenuator (BTLA) mRNA compared to controls, lower BTLA protein expression was observed in cases compared to controls. Hence we hypothesize that micro RNA (miR) 155-5p regulates BTLA expression in CLL. In line with earlier data, expression of BTLA mRNA and miR-155-5p is elevated in CLL (p = 0.034 and p = 0.0006, respectively) as well as in MEC-1 cell line (p = 0.009 and 0.016, respectively). Inhibition of miR-155-5p partially restored BTLA protein expression in CLL patients (p = 0.01) and in MEC-1 cell lines (p = 0.058). Additionally, we aimed to evaluate the significance of BTLA deficiency in CLL cells on proliferation and IL-4 production of B cells. We found that secretion of IL-4 is not dependent on BTLA expression, since fractions of BTLA positive and BTLA negative B cells expressing intracellular IL-4 were similar in CLL patients and controls. We demonstrated that in controls the fraction of proliferating cells is lower in BTLA positive than in BTLA negative B cells (p = 0.059), which was not observed in CLL. However, the frequency of BTLA positive Ki67+ B cells in CLL was higher compared to corresponding cells from controls (p = 0.055) while there were no differences between the examined groups regarding frequency of BTLA negative Ki67+ B cells. Our studies suggest that miR-155-5p is involved in BTLA deficiency, affecting proliferation of CLL B cells, which may be one of the mechanisms responsible for CLL pathogenesis.
Impact of SOCE Abolition by ORAI1 Knockout on the Proliferation, Adhesion, and Migration of HEK-293 Cells
Authors: Bokhobza Alexandre , Ziental-Gelus Nathalie , Allart Laurent , Iamshanova Oksana , Vanden Abeele Fabien
Published: November 2021

Store-operated calcium entry (SOCE) provided through channels formed by ORAI proteins is a major regulator of several cellular processes. In immune cells, it controls fundamental processes such as proliferation, cell adhesion, and migration, while in cancer, SOCE and ORAI1 gene expression are dysregulated and lead to abnormal migration and/or cell proliferation. In the present study, we used the CRISPR/Cas9 technique to delete the ORAI1 gene and to identify its role in proliferative and migrative properties of the model cell line HEK-293. We showed that ORAI1 deletion greatly reduced SOCE. Thereby, we found that this decrease and the absence of ORAI1 protein did not affect HEK-293 proliferation. In addition, we determined that ORAI1 suppression did not affect adhesive properties but had a limited impact on HEK-293 migration. Overall, we showed that ORAI1 and SOCE are largely dispensable for cellular proliferation, migration, and cellular adhesion of HEK-293 cells. Thus, despite its importance in providing Ca2+ entry in non-excitable cells, our results indicate that the lack of SOCE does not deeply impact HEK-293 cells. This finding suggests the existence of compensatory mechanism enabling the maintenance of their physiological function.
Single Fiber Reflectance Spectroscopy for the Monitoring of Cement Paste
Authors: da Silva Pedro M. , Coelho Luís C. C. , Almeida José M. M. M. de
Published: November 2021

Reinforced concrete structures are an essential part of our modern society, and monitoring their structural health is of paramount importance. Early detection of decay allows for the reduction of repair costs and, more importantly, the prevention of catastrophic failure. For this purpose, a single fiber reflectance spectrometer was embedded in cement paste samples for the monitoring of water at the fiber tip through its sensitivity to changes in the refractive index. It monitored the curing of samples with different water-to-cement ratios (w/c), between 0.45 and 0.60, measuring the water exhaust during the hardening of the cement paste. It also measured the capillary coefficient from cement paste samples of 0.50, 0.55 and 0.60 w/c: 0.668 ± 0.002 mm/√h, 1.771 ± 0.052 mm/√h and 6.360 ± 0.269 mm/√h, respectively. The capillary coefficient values agree with gravimetric measurements of sorptivity and are further confirmed through porosity measurements made with a scanning electron microscope. Thus, single fiber reflectance spectroscopy can be a gateway to inexpensively measure the entire life cycle of cement, from its curing until its eventual decay, assessing, in situ, its durability through the capillary coefficient.
Mesonephric-like Adenocarcinoma of the Uterine Corpus: Comprehensive Immunohistochemical Analyses Using Markers for Mesonephric, Endometrioid and Serous Tumors
Authors: Kim Hyunjin , Na Kiyong , Bae Go Eun , Kim Hyun-Soo
Published: November 2021

Mesonephric-like adenocarcinoma (MLA) of the uterine corpus is a rare but distinct malignant tumor of the female genital tract, demonstrating a characteristic morphology and unique immunohistochemical profiles and molecular alterations. We conducted immunohistochemical staining (IHC) to make precise differential diagnoses of uterine MLAs from common histological subtypes of endometrial carcinomas. We collected 25 uterine MLAs and performed IHC for GATA3, TTF1, CD10, ER, PR, p16, p53, and HER2. Seventeen cases (68.0%) showed at least moderate nuclear GATA3 immunoreactivity in ≥25% of tumor cells. Most cases expressed TTF1 (17/21, 81.0%) and CD10 (luminal; 17/21, 81.0%). Heterogeneous TTF1 expression was noted in 12 cases. An inverse pattern of GATA3 and TTF1 staining was observed in eight cases (32.0%). Three cases (12.0%) showed moderate-to-strong ER expression in ≥25% of tumor cells, and two cases (8.0%) showed moderate-to-strong PR expression in ≥5% of tumor cells. These hormone receptor-positive MLAs varied in intensity and proportion of GATA3 staining. None of the 25 cases exhibited either diffuse and strong p16 expression or aberrant p53 expression. Five cases (20.0%) showed equivocal HER2 immunoreactivity (score 2+), but HER2 FISH confirmed that none of them exhibited HER2 gene amplification. In summary, a small subset of uterine MLAs displayed atypical IHC results: focal but strong expression of ER or PR, the complete absence of GATA3 immunoreactivity, the concurrent expression of mesonephric and hormone receptors, and the inverse pattern of GATA3 and TTF1 staining. These unusual immunophenotypes may complicate the differential diagnosis of MLA. Moreover, pathologists should be encouraged to interpret the IHC results cautiously.
COVID-19 Diagnostics Outside and Inside the National Health Service: A Single Institutional Experience
Authors: Fulawka Lukasz , Kuzan Aleksandra
Published: November 2021

The COVID-19 epidemic has been going on continuously for more than 1.5 years. Fast and reliable diagnosis is a key component of an outbreak response strategy. Our goal is to present the statistics from one of the diagnostic points of a large city in Poland. Swabs of the throat or nasopharynx of people reporting for molecular diagnostics of SARS-CoV-2 presence were taken. CE-IVD-certified RNA isolation and RT-PCR assays were used. According to our data, the prevalence of SARS-CoV-2 infection in the examined population equaled 14.7%; however, large differences were observed depending on where the sampling point was located: as much as 50.3% of positive results for samples collected at a stationary point, 36.2% for samples from inpatients and hospital staff, and only 8.9% for samples from patients whose test was paid by their employer. The age structure of the infected population was fairly even, with a slightly higher number of people over 50 years of age. Men were examined more often, but it was among women that a higher percentage of infection was recorded. Every fifth test was performed for a foreigner, but compared to Poles, a much lower incidence of infection was found in these samples. We conclude that due to the high prevalence of infection in patients from social care centers and in those referred to hospitals, it is recommended that a special sanitary regime is followed in those settings. We will evaluate the effectiveness of vaccinations, expecting that the coming months bring positive changes in the statistics on prevalence.
The Month-of-the-Year Effect in the European, American, Australian and Asian Markets
Authors: Couto Gualter , Pimentel Pedro , Barbosa Catarina , Castanho Rui Alexandre
Published: November 2021

This paper examines the existence of the month-of-the-year effects in four different continents, namely Europe, Asia, America, and Oceania. Nine indexes were analyzed in order to verify differences between monthly returns from January 1990 to December 2013, followed by an examination of the January effect, Halloween effect, and the October effect, testing for statistical significance using an OLS linear regression in order to verify whether those effects offer consistent opportunities for investors. Investors with globally diversified portfolios benefit from the Halloween effect, with a 1.2% average monthly excess return in winter and spring, while the pre-dotcom-bubble period had a better performance than the post-dotcom-bubble period. In the global post-dotcom-bubble period, there is statistical evidence for 1.60% and 1% lower average monthly returns in January (the January effect) and in months other than October (the October effect), respectively, contradicting the literature. The dotcom bubble seems to be responsible for the January effect differing from what might otherwise have been expected in the later period. There is no consistent and clear impact on continental incidence. The Halloween effect is revealed to be a fruitful strategy in the FTSE, DAX, Dow Jones, BOVESPA, and N225 indexes taken one-by-one. The January effect excess average return was only statistically significative for the pre-dotcom-bubble period for globally diversified portfolios. This paper contributes to a wider global and comparable view upon month-of-the-year effect.
Multi-Task Learning with Task-Specific Feature Filtering in Low-Data Condition
Authors: Lee Sang-woo , Lee Ryong , Seo Min-seok , Park Jong-chan , Noh Hyeon-cheol , Ju Jin-gi , Jang Rae-young , Lee Gun-woo , Choi Myung-seok , Choi Dong-geol
Published: November 2021

Multi-task learning is a computationally efficient method to solve multiple tasks in one multi-task model, instead of multiple single-task models. MTL is expected to learn both diverse and shareable visual features from multiple datasets. However, MTL performances usually do not outperform single-task learning. Recent MTL methods tend to use heavy task-specific heads with large overheads to generate task-specific features. In this work, we (1) validate the efficacy of MTL in low-data conditions with early-exit architectures, and (2) propose a simple feature filtering module with minimal overheads to generate task-specific features. We assume that, in low-data conditions, the model cannot learn useful low-level features due to the limited amount of data. We empirically show that MTL can significantly improve performances in all tasks under low-data conditions. We further optimize the early-exit architecture by a sweep search on the optimal feature for each task. Furthermore, we propose a feature filtering module that selects features for each task. Using the optimized early-exit architecture with the feature filtering module, we improve the 15.937% in ImageNet and 4.847% in Places365 under the low-data condition where only 5% of the original datasets are available. Our method is empirically validated in various backbones and various MTL settings.
Challenges in Treatment of Digestate Liquid Fraction from Biogas Plant. Performance of Nitrogen Removal and Microbial Activity in Activated Sludge Process
Authors: Chuda Aleksandra , Ziemiński Krzysztof
Published: November 2021

Even thoughdigestate, which is continually generated in anaerobic digestion process, can only be used as fertilizer during the growing season, digestate treatment is still a critical, environmental problem. That is why the present work aims to develop a method to manage digestate in agricultural biogas plant in periods when its use as fertilizer is not possible. A lab-scale system for the biological treatment of the digestate liquid fraction using the activated sludge method with a separate denitrification chamber was constructed and tested. The nitrogen load that was added tothe digestate liquid fraction accounted for 78.53% of the total nitrogen load fed into the reactor. External carbon sources, such as acetic acid, as well as flume water and molasses, i.e., wastewater and by-products from a sugar factory, were used to support the denitrification process. The best results were obtained using an acetic acid and COD (Chemical Oxygen Demand)/NO3–N (Nitrate Nitrogen) ratio of 7.5. The removal efficiency of TN (Total Nitrogen), NH4–N (Ammonia Nitrogen) and COD was 83.73%, 99.94%, 86.26%, respectively. It was interesting to see results obtained that were similar to those obtained when using flume water and COD/NO3–N at a ratio of 8.7. This indicates that flume water can be used as an alternative carbon source to intensify biological nitrogen removal from digestate.
Modified Beetle Annealing Search (BAS) Optimization Strategy for Maxing Wind Farm Power through an Adaptive Wake Digraph Clustering Approach
Authors: Chen Yanfang , Joo Young-Hoon , Song Dongran
Published: November 2021

Owing to scale-up and complex wake effects, the centralized control that processes the command from turbines may be unsuitable, as it incurs high communication overhead and computational complexity for a large offshore wind farm (OWF). This paper proposes a novel decentralized non-convex optimization strategy for maxing power conversion of a large OWF based on a modified beetle antennae search (BAS) algorithm. First, an adaptive threshold algorithm which to establish a pruned wake direction graph while preserving the most critical wake propagation relationship among wind turbines are presented. The adaptive graph constraints were used to create wake sub-digraphs that split the wind farm into nearly uncoupled clustering communication subsets. On this basis, a Monte Carlo-based beetle annealing search (MC-BAS) nonlinear optimization strategy was secondly designed to adjust the yaw angles and axial factors for the maximum power conversion of each turbine subgroup. Finally, the simulation results demonstrated that a similar gain could be achieved as a centralized control method at power conversion and reduces the computational cost, allowing it to solve the nonlinear problem and real-time operations of the OWF.
Optimization of Biochar Production by Co-Torrefaction of Microalgae and Lignocellulosic Biomass Using Response Surface Methodology
Authors: Viegas Catarina , Nobre Catarina , Correia Ricardo , Gouveia Luísa , Gonçalves Margarida
Published: November 2021

Co-torrefaction of microalgae and lignocellulosic biomass was evaluated as a method to process microalgae sludge produced from various effluents and to obtain biochars with suitable properties for energy or material valorization. The influence of four independent variables on biochar yield and properties was evaluated by a set of experiments defined by response surface methodology (RSM). The biochars were characterized for proximate and ultimate composition, HHV, and methylene blue adsorption capacity. HHV of the biochars was positively correlated with carbonization temperature, residence time, and lignocellulosic biomass content in the feed. Co-torrefaction conditions that led to a higher yield of biochar (76.5%) with good calorific value (17.4 MJ Kg−1) were 250 °C, 60 min of residence time, 5% feed moisture, and 50% lignocellulosic biomass. The energy efficiency of the process was higher for lower temperatures (92.6%) but decreased abruptly with the increase of the moisture content of the feed mixture (16.9 to 57.3% for 70% moisture). Biochars produced using algal biomass grown in contaminated effluents presented high ash content and low calorific value. Dye removal efficiency by the produced biochars was tested, reaching 95% methylene blue adsorption capacity for the biochars produced with the least severe torrefaction conditions.
Energy Analysis of an Integrated Plant: Fluidized Bed Steam Gasification of Hydrothermally Treated Biomass Coupled to Solid Oxide Fuel Cells
Authors: Papa Alessandro Antonio , Di Carlo Andrea , Bocci Enrico , Taglieri Luca , Del Zotto Luca , Gallifuoco Alberto
Published: November 2021

An innovative process based on hydrothermal carbonization, gasification, and solid oxide fuel cells (SOFCs) technologies was developed using a commercial process simulation software called ASPEN Plus. The object of this work is to study plant efficiency under various operating conditions. The hydrothermal pre-treatment (HTC) at 200 and 250 °C was modelled as a black box based on the experimental results. The gasifier was modelled as a single reactor vessel with both the fluidized bed steam gasification of solid fuel and the hot gas cleaning system. The SOFC was modelled as a simple grey box with the ASPEN Plus blocks. The effect of HTC temperature and steam/carbon (S/C) ratio on the syngas composition and yield and plant efficiency was studied. The results show that the gasification of hydrochar obtained at 200 °C with S/C ratio of 0.6 gives the best results, namely an energy output of SOFC equal to 1.81 kW/kgBiomass, and overall process efficiency of 36%.
Challenges in the Electromagnetic Design of Multiphase Machines: Winding and Equivalent Circuit Parameters
Authors: Laksar Jan , Cermak Radek , Hruska Karel
Published: November 2021

The usage of multiphase electrical drives expands the operation possibilities of electrical machines and opens new directions of research on inverter-fed electrical machines. With an increasing number of phases, the standard approach of the electromagnetic design of machines has to be generalized to m-phase systems, which is not usually respected in the literature focused on electric machine design, and it is rarely published. This paper summarizes the specific problems linked with the design of machines with different numbers of phases, focusing on the winding design and the calculation of equivalent circuit parameters. In addition to the direct effect of different numbers of phases, the impact of injecting higher order time harmonic components on the electromagnetic design of electric machines is analyzed. The obtained analytical results are verified by the measurement of a nine-phase experimental induction motor.
Technical and Economic Aspects of Electric Energy Storage in a Mine Shaft—Budryk Case Study
Authors: Kulpa Jarosław , Kamiński Paweł , Stecuła Kinga , Prostański Dariusz , Matusiak Piotr , Kowol Daniel , Kopacz Michał , Olczak Piotr
Published: November 2021

The transformation of the energy sector towards an increased share of renewable energy sources in the energy mix requires attention in the area of electricity storage. Renewable energy sources (photovoltaics or wind energy) are marked by the intermittency of electricity production and require the construction of energy storage to adapt the energy supply to the demand, providing greater stability. The authors focused on verifying the solution of gravitational energy storage in existing shafts of hard coal mines in Poland. The issue is significant for Poland, as a country with an extensive mining infrastructure, which is searching for new scientific and practical solutions to utilize disused mining shafts for new purposes. In the analysis, the focus was on one shaft located within the Upper Silesian Coal Basin (Górnośląskie Zagłębie Węglowe), maintained for the drainage of the neighboring deposit. The article presents the calculation of energy that can be stored and an analysis of the effectiveness of energy storage in the shaft. The basic assumption of the analysis was 1 cycle of work per day (charging and discharging) in order to use the effect of low prices at night and high prices during peak hours (according to Towarowa Giełda Energii (TGE) quotations). Although energy storage already functions around the world, the studied case is new, because it refers to the usage of existing shafts which makes it a non-investment case. The results of the study showed that the obtained economic effects of the analyzed solution are low, therefore there is no economic justification of activities related to its implementation, taking into account the current price conditions.