bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Brosimum alicastrum Foliage on Intake, Kinetics of Fermentation and Passage and Microbial N Supply in Sheep Fed Megathyrsus maximus Hay
Authors: Valdivia-Salgado Vicente , Flores-Santiago Ever del Jesús , Ramírez-Avilés Luis , Segura-Correa José Candelario , Calzada-Marín Jesús Miguel , Ku-Vera Juan Carlos
Published: April 2024

An experiment was carried out to assess the effect of the incorporation of sun-dried foliage of Brosimum alicastrum into rations based on hay of Megathyrsus maximus on intake, rumen fermentation, kinetics of passage, microbial nitrogen supply to the small intestine, apparent digestibility in Pelibuey hair sheep. Four rations were randomly allotted to four rumen-cannulated lambs (BW = 37.4 ± 4.9 kg) using a 4 × 4 Latin square design to assess the effect of increasing levels (0, 15, 30 and 45% DM basis) of foliage of Brosimum alicastrum on a basal ration of M. maximus. Organic matter intake and water consumption increased linearly (p < 0.01) with increasing levels of B. alicastrum in the ration. The rate and potential extent of rumen fermentation of OM and CP of B. alicastrum were 10.6%/h and 86.6% and 11.4%/h and 95.2%, respectively, but no effect (p > 0.05) was found on the potential rumen degradation of DM (40.2%) or on the rate of degradation of DM (0.033%/h) of M. maximus, although a positive effect was found in the rumen degradation rate of NDF (p < 0.05). VFA and ammonia concentration in the rumen and the rate of passage of solids and liquids through the rumen (k1) increased linearly (p < 0.01) with increasing levels of B. alicastrum. Rumen pH was not affected by the incorporation of B. alicastrum (p > 0.05). Microbial nitrogen supply to the small intestine (p < 0.001), apparent digestibility of dry matter (p < 0.01) and NDF (p < 0.05) of the rations were also significantly increased as a result of the incorporation of B. alicastrum foliage. Results from this experiment suggest that the foliage of Brosimum alicastrum can be readily incorporated at around 30% of the ration of dry matter in hair sheep with beneficial effects on feed intake, rate of passage and microbial N supply to the lower tract.
Genome-Wide Association Study of Body Conformation Traits in Tashi Goats (Capra hircus)
Authors: Yang Rong , Zhou Di , Tan Xiaoshan , Zhao Zhonghai , Lv Yanli , Tian Xingzhou , Ren Liqun , Wang Yan , Li Jun , Zhao Yongju , Zhang Jipan
Published: April 2024

Identifying genetic markers of economically valuable traits has practical benefits for the meat goat industry. To better understand the genomic variations influencing body conformation traits, a genome-wide association study was performed on Tashi goats, an indigenous Chinese goat breed. A total of 155 Tashi goats were phenotyped for eight body conformation traits: body height, body length, chest depth, chest width, chest girth, rump width, rump height, and cannon bone circumference. Then, 100 Tashi goats were randomly selected for whole-genome sequencing and genotyped. We obtained 1676.4 Gb of raw data with an average sequencing depth of 6.2X. Clean reads were aligned to the ARS1.2 reference genome, and 11,257,923 single nucleotide polymorphisms (SNPs) were identified. The structure analysis showed that these Tashi goats were almost not genetically related. The 109, 20, 52, 14, 62, 51, 70, and 7 SNPs were significantly associated with body height, body length, chest depth, chest width, chest girth, rump width, rump height, and cannon bone circumference. Within the ±500 kb region of significant SNPs, 183 genes were annotated. The most significantly enriched KEGG pathway was “olfactory transduction”, and the most significantly enriched gene ontology (GO) terms were “cellular process”, “cellular anatomical entity”, and “molecular transducer activity”. Interestingly, we found several SNPs on chromosomes 10 and 11 that have been identified multiple times for all eight body conformation traits located in two fragments (114 kb and 1.03 Mb). In chr.10:25988403-26102739, the six SNPs were tightly linked, the TACTAG genotype was the highest at 91.8%, and the FNTB (Farnesyltransferase, CAAX Box Beta) and CHURC1 (Churchill Domain Containing 1) genes were located. In chr.11:88216493-89250659, ten SNPs were identified with several dependent linkage disequilibrium (LD) blocks, and seven related genes were annotated, but no significant SNP was located in them. Our results provide valuable biological information for improving growth performance with practical applications for genomic selection in goats.
Application of 3D Imaging for Analyzing the Chip Groove Shapes of Cutting Inserts
Authors: Struzikiewicz Grzegorz
Published: April 2024

An effective chip formation process is significant for an efficient metal-cutting process. Long continuous chips can lead to scratches on the machined surface, increasing the risk to operator safety and stability of the machining process. The use of chip grooves on cutting inserts allows for control of the chip formation and breaking process during machining. The shape of the rake surface and the design of the chip groove also affect the efficiency of the machining process. The article presents the use of 3D imaging to analyze changes in the selected chip groove shapes depending on the cutting depth ap = 0.10, 0.25, and 0.50 mm and the angular location of the cutting insert relative to the machined surface of the workpiece (i.e., major cutting-edge angle K = 60° and K = 90°). The analysis methodology was based on the use of 3D image registration and surface shape modeling. In the analysis based on the 3D imaging presented, the novelty was the adaptation of methods typically used to map and model the terrain surface, which have not been used previously in cutting processes. The evaluation of the shape of the chip groove surface was carried out using, e.g., watershed maps and 3D surface maps. The obtained results indicated a significant influence of the cutting depth and major cutting-edge angle on the surface shape, profile, and length of the chip former; chip groove volume; and the theoretical contact area of the formed chip with the cutting insert. It was observed that for small depths of cut, i.e., ap < 0.25 mm, the chip-curling process may be difficult due to the flattened shape of the rake surface. In addition, the influence of the convexity of the rake surface of the cutting insert on the chip formation process was demonstrated. The results of the experimental research that verified the conclusions are presented. The developed results may be useful in the process of selecting the parameters and conditions of the metal finishing through use of tools with a shaped rake surface.
Identification of the Surface Cracks of Concrete Based on ResNet-18 Depth Residual Network
Authors: Wang Rong , Zhou Xinyang , Liu Yi , Liu Dongqi , Lu Yu , Su Miao
Published: April 2024

To ensure the safety and durability of concrete structures, timely detection and classification of concrete cracks using a low-cost and high-efficiency method is necessary. In this study, a concrete surface crack damage detection method based on the ResNet-18 residual network was developed. This method was implemented by training a model with images to extract the cracks, where the image processing algorithms and deep learning were combined. The results show that the computational accuracy can meet the requirements by utilizing the established image dataset and appropriate model hyperparameters. The trained model had high recognition accuracy when the 256 × 256 resolution images were adopted, and the worst accuracy of crack recognition in the test set was over 90%. The average accuracy in the test set was 91.3% when considering environmental interference generated by processing the images with a brightness adjustment, salt-and-pepper noise, and localized interference. Then, it was demonstrated that the present model possesses good robustness for crack identification in different environments. The average recognition accuracy when dealing with images of a real bridge, which are outside the training dataset, was 99.7%. The residual network model developed in this study has the advantages of low cost, high efficiency, and practicality compared to traditional detection methods. Compared to the existing deep learning methods, the model created in this study requires less computational resources and storage space, and shows a faster training speed and higher accuracy.
Cognitive and Motor Capacities Are Poorly Correlated with Agility in Early Pubertal Children: Gender-Stratified Analysis
Authors: Pavlinović Vladimir , Foretić Nikola , Kovačević Neven , Galić Tea , Lušić Kalcina Linda , Mihanović Frane , Modric Toni
Published: April 2024

This research aimed to identify relations of cognitive and power capacities with reactive agility in pubescent boys (n = 55) and girls (n = 46). Cognitive abilities were evaluated by the Stroop test, while the BlazePod system was used to evaluate agility performance conducting 20 yard shuttle and triangle tests of non-reactive (TCODS) and reactive agility (TRAG), respectively. Performance in jumping power was assessed through the squat jump (SJ), countermovement jump (CMJ), and drop jump (DHJ) utilising the Opto Jump system (Microgate, Bolzano, Italy), while sprinting ability over distances of 10 and 20 m was measured using a photocells system. A principal component was extracted from the four Stroop test variables using factor analysis. Forward stepwise multiple regression analysis was conducted separately for boys and girls to evaluate the multivariate relationships among the predictors and the criterion. Among boys, 80% of the TRAG variance was explained (MultipleR = 0.9), with TCODS and SJ as significant predictors (β = 0.53 and −1.01, respectively). For girls, the TCODS was the significant predictor (β = 0.65), explaining 43% of the variance (MultipleR = 0.65). These results show that (i) cognitive abilities measured with the Stroop test were not a reliable tool for predicting TRAG, (ii) jumping power was a significant predictor of TRAG in boys, and (iii) TCODS was a significant predictor of TRAG in girls. The findings indicated that cognitive abilities do not significantly influence reactive agility in pubescent children. It seems that power features have a greater influence on reactive agility, particularly in boys who have more developed motor skills at this age compared to girls.
A New Approach to Understanding Quantum Mechanics: Illustrated Using a Pedagogical Model over ℤ2
Authors: Ellerman David
Published: April 2024

The new approach to quantum mechanics (QM) is that the mathematics of QM is the linearization of the mathematics of partitions (or equivalence relations) on a set. This paper develops those ideas using vector spaces over the field Z2={0.1} as a pedagogical or toy model of (finite-dimensional, non-relativistic) QM. The 0,1-vectors are interpreted as sets, so the model is “quantum mechanics over sets” or QM/Sets. The key notions of partitions on a set are the logical-level notions to model distinctions versus indistinctions, definiteness versus indefiniteness, or distinguishability versus indistinguishability. Those pairs of concepts are the key to understanding the non-classical ‘weirdness’ of QM. The key non-classical notion in QM is the notion of superposition, i.e., the notion of a state that is indefinite between two or more definite- or eigen-states. As Richard Feynman emphasized, all the weirdness of QM is illustrated in the double-slit experiment, so the QM/Sets version of that experiment is used to make the key points.
Sums of Independent Circular Random Variables and Maximum Likelihood Circular Uniformity Tests Based on Nonnegative Trigonometric Sums Distributions
Authors: Fernández-Durán Juan José , Gregorio-Domínguez María Mercedes
Published: April 2024

The sum of independent circular uniformly distributed random variables is also circular uniformly distributed. In this study, it is shown that a family of circular distributions based on nonnegative trigonometric sums (NNTS) is also closed under summation. Given the flexibility of NNTS circular distributions to model multimodality and skewness, these are good candidates for use as alternative models to test for circular uniformity to detect different deviations from the null hypothesis of circular uniformity. The circular uniform distribution is a member of the NNTS family, but in the NNTS parameter space, it corresponds to a point on the boundary of the parameter space, implying that the regularity conditions are not satisfied when the parameters are estimated by using the maximum likelihood method. Two NNTS tests for circular uniformity were developed by considering the standardised maximum likelihood estimator and the generalised likelihood ratio. Given the nonregularity condition, the critical values of the proposed NNTS circular uniformity tests were obtained via simulation and interpolated for any sample size by the fitting of regression models. The validity of the proposed NNTS circular uniformity tests was evaluated by generating NNTS models close to the circular uniformity null hypothesis.
Phylogeography of a Widely Distributed Atlantic Species: The Case of the Ghost Crab Ocypode quadrata (Fabricius, 1787) (Decapoda: Brachyura: Ocypodidae)
Authors: Tamburus Ana Francisca , Miranda Ivana , Naves Bárbara Benati , Mantelatto Fernando Luis
Published: April 2024

Ocypode Weber, 1795 (Brachyura: Ocypodidae) is popularly known as ghost crab, and encompasses 21 valid species, including Ocypode quadrata (Fabricius, 1787). This species has wide distribution along the Atlantic coast of America, from the USA (Massachusetts) to Brazil (Rio Grande do Sul), Central America, and Antilles. Such distribution, along with some biological characteristics of its life cycle and the presence of geographic barriers, could lead to genetic structuring. Herein, we evaluate the hypothesis of the presence of geographic barriers using COI and 16S partial gene fragments. The Maximum Likelihood tree suggests the monophyly of O. quadrata, while the values of intraspecific genetic distance along with the star-shaped haplotype network suggested a lack of genetic structure in Brazilian, Panama, and French Guiana populations, probably caused by larval dispersion. USA and Mexico populations may be a new lineage, but we cannot say it with few sequences and with no morphological characters.
A Comprehensive Analysis of Trapezius Muscle EMG Activity in Relation to Stress and Meditation
Authors: Ahmed Mohammad , Grillo Michael , Taebi Amirtaha , Kaya Mehmet , Thibbotuwawa Gamage Peshala
Published: April 2024

Introduction: This study analyzes the efficacy of trapezius muscle electromyography (EMG) in discerning mental states, namely stress and meditation. Methods: Fifteen healthy participants were monitored to assess their physiological responses to mental stressors and meditation. Sensors were affixed to both the right and left trapezius muscles to capture EMG signals, while simultaneous electroencephalography (EEG) was conducted to validate cognitive states. Results: Our analysis of various EMG features, considering frequency ranges and sensor positioning, revealed significant changes in trapezius muscle activity during stress and meditation. Notably, low-frequency EMG features facilitated enhanced stress detection. For accurate stress identification, sensor configurations can be limited to the right trapezius muscle. Furthermore, the introduction of a novel method for determining asymmetry in EMG features suggests that applying sensors on bilateral trapezius muscles can improve the detection of mental states. Conclusion: This research presents a promising avenue for efficient cognitive state monitoring through compact and convenient sensing.
Experimental Investigation on Aerodynamic Performance of Inclined Hovering with Asymmetric Wing Rotation
Authors: Zheng Mengzong , Peng Liansong , Su Guanting , Pan Tianyu , Li Qiushi
Published: April 2024

This study presents a model experiment method that can accurately reproduce the flapping motion of insect wings and measure related unsteady aerodynamic data in real time. This method is applied to investigate the aerodynamic characteristics of inclined hovering, which distinguishes it from normal hovering by having asymmetric wing rotation during the two half strokes. In the study of the aerodynamic influence of the downstroke rotational angle, it is found that the rotational angle affects lift generation by changing the angle between the wing surface and the horizontal plane in the mid-downstroke. When the wing is almost parallel to the horizontal plane in the mid-downstroke, the vortex structure can maintain structural integrity and a large magnitude, which is conducive to the generation of high lift. In the study of the aerodynamic effect of the upstroke rotational angle, the windward conversion mechanism is proposed to explain the influence of the upstroke rotational angle on the direction and magnitude of thrust. Obtaining the rotational angle that is most conducive to maintaining the flight state of hovering in the present study can provide guidance for the structural design and kinematic control of micro aerial vehicles.
A Study of Soundscape Restoration in Office-Type Pocket Parks
Authors: Wang Yaping , Deng Xin , Cui Yanqiu , Zhao Xin
Published: April 2024

High-density building environments and fast-paced working conditions in cities pose health challenges for office workers. Office-type pocket parks assume the social responsibility of providing restorative environments for office workers, and the soundscape is an essential element of such environments. However, there is limited research on soundscape restoration in office-type pocket parks. Therefore, this study focused on soundscape restoration in office-type pocket parks. First, on-site investigations explored the spatial characteristics of 55 office-type pocket parks while analysing the soundscape features of 12 representative parks. Notably, significant correlations emerged among the perceptual parameters of the soundscape. Subsequently, three dimensions were extracted through an experimental study on the restoration levels of soundscape elements in office-based pocket parks: attractiveness, coordination, and disengagement. Finally, this study explored the impact of spatial enclosures and interface characteristics on soundscape restoration levels. This revealed that the restorative effect of negative sounds, such as traffic, air-conditioning, and speech, is significantly negatively correlated with spatial enclosure. Therefore, the greater the enclosure, the lower the restorative effect of the soundscape. Birdsongs significantly enhance the attractiveness of grey spaces, whereas small fountain sounds are most coordinated in blue spaces. This study provides a reference for the design of soundscapes in pocket parks to build healthy, restorative urban environments.
The Prognostic Impact of the Surgical Margin in Renal Cell Carcinoma Treated with Partial Nephrectomy: A Multi-Center Study
Authors: Mosholt Karina Sif Søndergaard , Aagaard Mark , Røder Andreas , Azawi Nessn
Published: April 2024

Background: Partial nephrectomy (PN) is the preferred treatment for small, localized kidney tumors. Incomplete resection resulting in positive surgical margins (PSM) can occur after PN. The impact of PSM on the risk of recurrence and survival outcomes is not fully understood. We aimed to explore the relationship between PSM, the risk of recurrence and impact on survival after PN in a large multicenter cohort from Denmark. Methods: This was a retrospective cohort study including patients who underwent PN for renal cell carcinoma (RCC) at three departments in Denmark between 2010 and 2016. Data including pathological features, surgical techniques, and patient follow-up was retrieved from electronic medical health records and national databases. We used a combination of descriptive statistics, comparative analysis (comparisons were carried out by Mann–Whitney Test, independent Student’s t-test, or Pearson’s chi-Square Test), univariate and multivariate logistic regression analyses, and survival analysis methods. Results: A total of 523 patients were included, of which 48 (9.1%) had a PSM. Recurrence was observed in 55 patients (10.5%). Median follow-up time was 75 months. We found a lower incidence of PSM with robot-assisted PN (p = 0.01) compared to open or laparoscopic PN. PSM was associated with a higher risk of recurrence compared to negative margins in univariate analysis, but not multivariate analysis. However, the study was underpowered to describe this association with other risk factors. Overall survival did not differ between patients with PSM and negative margins. Conclusions: Our study presents further evidence on the negative impact of PSM on recurrence after PN for RCC, highlighting the importance of achieving NSM, thus potentially improving clinical outcomes. A surgical approach was found to be the only predictive factor influencing the risk of PSMs, with a reduced risk observed with robot-assisted laparoscopy.
Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater
Authors: Masood Thannoon , Lakatos Szandra , Kis Gyöngyi , Ignácz Melissza , Domoki Ferenc , Rosta Judit
Published: April 2024

Subarachnoid hemorrhage (SAH) remains a major cause of cerebrovascular morbidity, eliciting severe headaches and vasospasms that have been shown to inversely correlate with vasodilator calcitonin gene-related peptide (CGRP) levels. Although dura mater trigeminal afferents are an important source of intracranial CGRP, little is known about the effects of SAH on these neurons in preclinical models. The present study evaluated changes in CGRP levels and expression in trigeminal primary afferents innervating the dura mater 72 h after experimentally induced SAH in adult rats. SAH, eliciting marked damage revealed by neurological examination, significantly reduced the density of CGRP-immunoreactive nerve fibers both in the dura mater and the trigeminal caudal nucleus in the medulla but did not affect the total dural nerve fiber density. SAH attenuated ex vivo dural CGRP release by ~40% and in the trigeminal ganglion, reduced both CGRP mRNA levels and the number of highly CGRP-immunoreactive cell bodies. In summary, we provide novel complementary evidence that SAH negatively affects the integrity of the CGRP-expressing rat trigeminal neurons. Reduced CGRP levels suggest likely impaired meningeal neurovascular functions contributing to SAH complications. Further studies are to be performed to reveal the importance of impaired CGRP synthesis and its consequences in central sensory processing.
Sit4 Genetically Interacts with Vps27 to Regulate Mitochondrial Function and Lifespan in Saccharomyces cerevisiae
Authors: Martins Telma S. , Correia Miguel , Pinheiro Denise , Lemos Carolina , Mendes Marta Vaz , Pereira Clara , Costa Vítor
Published: April 2024

The Sit4 protein phosphatase plays a key role in orchestrating various cellular processes essential for maintaining cell viability during aging. We have previously shown that SIT4 deletion promotes vacuolar acidification, mitochondrial derepression, and oxidative stress resistance, increasing yeast chronological lifespan. In this study, we performed a proteomic analysis of isolated vacuoles and yeast genetic interaction analysis to unravel how Sit4 influences vacuolar and mitochondrial function. By employing high-resolution mass spectrometry, we show that sit4Δ vacuolar membranes were enriched in Vps27 and Hse1, two proteins that are part of the endosomal sorting complex required for transport-0. In addition, SIT4 exhibited a negative genetic interaction with VPS27, as sit4∆vps27∆ double mutants had a shortened lifespan compared to sit4∆ and vps27∆ single mutants. Our results also show that Vps27 did not increase sit4∆ lifespan by improving protein trafficking or vacuolar sorting pathways. However, Vps27 was critical for iron homeostasis and mitochondrial function in sit4∆ cells, as sit4∆vps27∆ double mutants exhibited high iron levels and impaired mitochondrial respiration. These findings show, for the first time, cross-talk between Sit4 and Vps27, providing new insights into the mechanisms governing chronological lifespan.
The Temporal Relationship between Blood–Brain Barrier Integrity and Microglial Response following Neonatal Hypoxia Ischemia
Authors: Jithoo Arya , Penny Tayla R. , Pham Yen , Sutherland Amy E. , Smith Madeleine J. , Petraki Maria , Fahey Michael C. , Jenkin Graham , Malhotra Atul , Miller Suzanne L. , McDonald Courtney A.
Published: April 2024

Blood–brain barrier (BBB) dysfunction and neuroinflammation are key mechanisms of brain injury. We performed a time-course study following neonatal hypoxia–ischemia (HI) to characterize these events. HI brain injury was induced in postnatal day 10 rats by single carotid artery ligation followed by hypoxia (8% oxygen, 90 min). At 6, 12, 24, and 72 h (h) post-HI, brains were collected to assess neuropathology and BBB dysfunction. A significant breakdown of the BBB was observed in the HI injury group compared to the sham group from 6 h in the cortex and hippocampus (p < 0.001), including a significant increase in albumin extravasation (p < 0.0033) and decrease in basal lamina integrity and tight-junction proteins. There was a decrease in resting microglia (p < 0.0001) transitioning to an intermediate state from as early as 6 h post-HI, with the intermediate microglia peaking at 12 h (p < 0.0001), which significantly correlated to the peak of microbleeds. Neonatal HI insult leads to significant brain injury over the first 72 h that is mediated by BBB disruption within 6 h and a transitioning state of the resident microglia. Key BBB events coincide with the appearance of the intermediate microglial state and this relationship warrants further research and may be a key target for therapeutic intervention.
Radiogenomics and Texture Analysis to Detect von Hippel–Lindau (VHL) Mutation in Clear Cell Renal Cell Carcinoma
Authors: Greco Federico , D’Andrea Valerio , Beomonte Zobel Bruno , Mallio Carlo Augusto
Published: April 2024

Radiogenomics, a burgeoning field in biomedical research, explores the correlation between imaging features and genomic data, aiming to link macroscopic manifestations with molecular characteristics. In this review, we examine existing radiogenomics literature in clear cell renal cell carcinoma (ccRCC), the predominant renal cancer, and von Hippel–Lindau (VHL) gene mutation, the most frequent genetic mutation in ccRCC. A thorough examination of the literature was conducted through searches on the PubMed, Medline, Cochrane Library, Google Scholar, and Web of Science databases. Inclusion criteria encompassed articles published in English between 2014 and 2022, resulting in 10 articles meeting the criteria out of 39 initially retrieved articles. Most of these studies applied computed tomography (CT) images obtained from open source and institutional databases. This literature review investigates the role of radiogenomics, with and without texture analysis, in predicting VHL gene mutation in ccRCC patients. Radiogenomics leverages imaging modalities such as CT and magnetic resonance imaging (MRI), to analyze macroscopic features and establish connections with molecular elements, providing insights into tumor heterogeneity and biological behavior. The investigations explored diverse mutations, with a specific focus on VHL mutation, and applied CT imaging features for radiogenomic analysis. Moreover, radiomics and machine learning techniques were employed to predict VHL gene mutations based on CT features, demonstrating promising results. Additional studies delved into the relationship between VHL mutation and body composition, revealing significant associations with adipose tissue distribution. The review concludes by highlighting the potential role of radiogenomics in guiding targeted and selective therapies.
Extracellular Vesicles Isolated from Equine Adipose-Derived Stromal Stem Cells (ASCs) Mitigate Tunicamycin-Induced ER Stress in Equine Corneal Stromal Stem Cells (CSSCs)
Authors: Meissner Justyna M. , Chmielińska Aleksandra , Ofri Ron , Cisło-Sankowska Anna , Marycz Krzysztof
Published: April 2024

Corneal ulcers, characterized by severe inflammation of the cornea, can lead to serious, debilitating complications and may be vision-threatening for horses. In this study, we aimed to investigate the role of endoplasmic reticulum (ER) stress in corneal stem progenitor cell (CSSC) dysfunction and explore the potential of equine adipose-derived stromal stem cell (ASC)-derived extracellular vesicles (EVs) to improve corneal wound healing. We showed that CSSCs expressed high levels of CD44, CD45, and CD90 surface markers, indicating their stemness. Supplementation of the ER-stress-inducer tunicamycin to CSSCs resulted in reduced proliferative and migratory potential, accumulation of endoplasmic reticulum (ER)-stressed cells in the G0/G1 phase of the cell cycle, increased expression of proinflammatory genes, induced oxidative stress and sustained ER stress, and unfolded protein response (UPR). Importantly, treatment with EVs increased the proliferative activity and number of cells in the G2/Mitosis phase, enhanced migratory ability, suppressed the overexpression of proinflammatory cytokines, and upregulated the anti-inflammatory miRNA-146a-5p, compared to control and/or ER-stressed cells. Additionally, EVs lowered the expression of ER-stress master regulators and effectors (PERK, IRE1, ATF6, and XBP1), increased the number of mitochondria, and reduced the expression of Fis-1 and Parkin, thereby promoting metabolic homeostasis and protecting against apoptosis in equine CSSCs. Our findings demonstrate that MSCs-derived EVs represent an innovative and promising therapeutic strategy for the transfer of bioactive mediators which regulate various cellular and molecular signaling pathways.
The Vitamin K-Dependent Anticoagulant Factor, Protein S, Regulates Vascular Permeability
Authors: Joussaume Aurélie , Kanthou Chryso , Pardo Olivier E. , Karayan-Tapon Lucie , Benzakour Omar , Dkhissi Fatima
Published: April 2024

Protein S (PROS1) is a vitamin K-dependent anticoagulant factor, which also acts as an agonist for the TYRO3, AXL, and MERTK (TAM) tyrosine kinase receptors. PROS1 is produced by the endothelium which also expresses TAM receptors, but little is known about its effects on vascular function and permeability. Transwell permeability assays as well as Western blotting and immunostaining analysis were used to monitor the possible effects of PROS1 on both endothelial cell permeability and on the phosphorylation state of specific signaling proteins. We show that human PROS1, at its circulating concentrations, substantially increases both the basal and VEGFA-induced permeability of endothelial cell (EC) monolayers. PROS1 induces p38 MAPK (Mitogen Activated Protein Kinase), Rho/ROCK (Rho-associated protein kinase) pathway activation, and actin filament remodeling, as well as substantial changes in Vascular Endothelial Cadherin (VEC) distribution and its phosphorylation on Ser665 and Tyr685. It also mediates c-Src and PAK-1 (p21-activated kinase 1) phosphorylation on Tyr416 and Ser144, respectively. Exposure of EC to human PROS1 induces VEC internalization as well as its cleavage into a released fragment of 100 kDa and an intracellular fragment of 35 kDa. Using anti-TAM neutralizing antibodies, we demonstrate that PROS1-induced VEC and c-Src phosphorylation are mediated by both the MERTK and TYRO3 receptors but do not involve the AXL receptor. MERTK and TYRO3 receptors are also responsible for mediating PROS1-induced MLC (Myosin Light Chain) phosphorylation on a site targeted by the Rho/ROCK pathway. Our report provides evidence for the activation of the c-Src/VEC and Rho/ROCK/MLC pathways by PROS1 for the first time and points to a new role for PROS1 as an endogenous vascular permeabilizing factor.
Influence of the Nature of Cement on the Physical and Mechanical Properties of Soil Concretes from Sandy Clay and Laterite
Authors: Kamdem Alain , Elat Emmanuel , Eslami Javad , Amba Jean Chills , Sali Moussa , Mbessa Michel , Noumowé Albert
Published: April 2024

Soil concrete is a material produced by mixing the soil at the site with a hydraulic binder. This paper aims to study the influence of the nature of binder on the physical and mechanical properties of soil concrete. For the mixtures, three types of soil were chosen and studied: sandy clay with a granular class of 0/5 (SA5), laterite with a granular class of 0/5 (LA5), and laterite with a granular class of 0/10 (LA10). Three different cements were used: CEM I 52.5, CEM II 42.5, and CEM III 32.5, with cement contents of 150 and 250 kg/m3. The soil concretes were designed for a constant spread of 32–33 cm measured on a mini-slump. The results showed that LA5-based soil concrete has a higher water content of about 8.8% more than SA5 and LA10-based soil concretes. For all the mixtures, the lowest porosity values were obtained with CEM III 32.5, followed by CEM I 52.5, and finally CEM II 42.5. For the three types of cement and the same soil granular size, the compressive strength, static, and dynamic modulus of SA5-based soil concretes are higher than LA5. It was noted that the mechanical properties of soil concretes made with CEM III 32.5 are higher than those made with CEM I 52.5 and CEM II 42.5. Regardless of the type of cement used, the mechanical properties obtained on LA10-based soil concrete are higher than those on LA5-based soil concrete.
Neighborhood Light at Night and Noise Levels, and Long-Term Sleep Trajectories in the Southern Community Cohort Study
Authors: Nyarko Samuel H. , Xiao Qian
Published: April 2024

While light at night (LAN) and noise levels have been linked to suboptimal sleep outcomes, little is known about the link between these factors and long-term suboptimal sleep trajectories. The current study examined the association of neighborhood LAN and nighttime noise with long-term sleep trajectories in a cohort of Black individuals and White individuals predominantly from low-income communities. We used data from the Southern Community Cohort Study (N = 28,759 Black individuals and 16,276 White individuals). Sleep duration was self-reported at baseline and after an average of five years of follow-up, based on which we constructed nine sleep trajectories: normal–normal (optimal, reference), short–short, long–long, short–long, long–short, normal–short, normal–long, short–normal, and long–normal. LAN and nighttime noise were derived from satellite imagery and model-based estimates, respectively. Multinomial logistic regression was used to determine the relationship between LAN and noise exposures and sleep trajectories. Higher exposures to LAN and nighttime noise were associated with multiple suboptimal long-term sleep trajectories. In the total sample, higher LAN was associated with higher odds of long–long (OR Q5 vs. Q1 = 1.23 (CI = 1.02, 1.48)) and long–short (OR = 1.35 (CI = 1.06, 1.72)) trajectories, while higher nighttime noise was associated with short–short (1.19 (1.07, 1.31)), long–short (1.31 (1.05, 1.64)), and normal–song (1.16 (1.01, 1.34)) trajectories. Black and White individual-specific results showed qualitatively similar patterns between Black individuals and White individuals, although we also observed suggestive evidence for Black–White individual differences. In conclusion, elevated LAN and nighttime noise levels were associated with various suboptimal long-term sleep trajectories. However, it is noteworthy that the light and noise measures in our study may not accurately reflect individual-level exposures, and residual confounding from other factors is a concern. Future studies should use more accurate exposure measurements, collect information on and control for a wider range of factors, and examine whether reductions in neighborhood light and noise levels may contribute to improved long-term sleep health.
Influence of Cylindrical Cells Surface Cleaning by Means of Laser Ablation on Wedge Wire Bonding Process
Authors: Bieliszczuk Krzysztof , Zręda Jakub , Chmielewski Tomasz M.
Published: April 2024

Wire bonding is a method of connecting two or more surfaces by the means of a thin wire which is ultrasonically bonded to those surfaces and provides an electrical connection. While this method is well established in the microelectronics industry its popularity is rising in the area of cylindrical lithium-ion battery pack manufacturing. Previous studies have shown that even in experimental conditions this process might be unstable which was indicated by the high standard deviation of the bonds shear test results. This might have been related to contamination of the interface area between the joined materials. The aim of this study was to determine the impact of surface laser cleaning on the properties of the wire-bonded joint. The results have shown that laser cleaning with 40% power of the 30 W ATMS4060 laser marker helps to reduce the standard deviation of the shear test results from 16.1% for the uncleaned sample down to 2.6% and greatly reduces the number of oxides within the interface area of the bond cross section. Cleaning with 80% of the laser power did not have a further impact on shear test results and almost completely eliminated oxides from the bonded materials interface.
Cu/Mn Synergy Catalysis-Based Colorimetric Sensor for Visual Detection of Hydroquinone
Authors: Xing Ningning , Yang Lilin , Wang Li , Lu Yanxiang , Zhang Hongkun , Sun Xijun , Zhao Min , Tan Wenjie , Yang Jie
Published: April 2024

The reliable detection of environmental contaminants can correctly forecast the degree of environmental pollution that has occurred, which contributes to improving the environmental purification rate and maintaining the ecological balance. Herein, a novel hierarchical biomimetic catalysis MnO2@CuAl-CLDHs was designed and synthesized using a facile method, which exhibited significantly enhanced peroxidase-like activity due to the unique composition and hierarchical mesoporous structure. Under optimized operational conditions, a visible colorimetric array based on the superior nanozyme activity of MnO2@CuAl-CLDHs was developed for the quantitative determination of hydroquinone with a wide linear detection range (1–100 μM) and a low detection limit (0.183 μM). Simultaneously, our presented strategy could analyze hydroquinone in real water samples with high accuracy. Therefore, the bimetallic co-catalyzed nanozymes are expected to be the perfect replacement for natural enzymes to develop convenient and efficient sensors.
Interdisciplinary Collaboration in Head and Neck Cancer Care: Optimizing Oral Health Management for Patients Undergoing Radiation Therapy
Authors: Kutuk Tugce , Atak Ece , Villa Alessandro , Kalman Noah S. , Kaiser Adeel
Published: April 2024

Radiation therapy (RT) plays a crucial role in the treatment of head and neck cancers (HNCs). This paper emphasizes the importance of effective communication and collaboration between radiation oncologists and dental specialists in the HNC care pathway. It also provides an overview of the role of RT in HNC treatment and illustrates the interdisciplinary collaboration between these teams to optimize patient care, expedite treatment, and prevent post-treatment oral complications. The methods utilized include a thorough analysis of existing research articles, case reports, and clinical guidelines, with terms such as ‘dental management’, ‘oral oncology’, ‘head and neck cancer’, and ‘radiotherapy’ included for this review. The findings underscore the significance of the early involvement of dental specialists in the treatment planning phase to assess and prepare patients for RT, including strategies such as prophylactic tooth extraction to mitigate potential oral complications. Furthermore, post-treatment oral health follow-up and management by dental specialists are crucial in minimizing the incidence and severity of RT-induced oral sequelae. In conclusion, these proactive measures help minimize dental and oral complications before, during, and after treatment.
Symptom Burden and Time from Symptom Onset to Cancer Diagnosis in Patients with Early-Onset Colorectal Cancer: A Multicenter Retrospective Analysis
Authors: Baronas Victoria A. , Arif Arif A. , Bhang Eric , Ladua Gale K. , Brown Carl J. , Donnellan Fergal , Gill Sharlene , Stuart Heather C. , Loree Jonathan M.
Published: April 2024

Background: The incidence of colorectal cancer (CRC) is decreasing in individuals >50 years due to organised screening but has increased for younger individuals. We characterized symptoms and their timing before diagnosis in young individuals. Methods: We identified all patients diagnosed with CRC between 1990–2017 in British Columbia, Canada. Individuals <50 years (n = 2544, EoCRC) and a matched cohort >50 (n = 2570, LoCRC) underwent chart review to identify CRC related symptoms at diagnosis and determine time from symptom onset to diagnosis. Results: Across all stages of CRC, EoCRC presented with significantly more symptoms than LoCRC (Stage 1 mean ± SD: 1.3 ± 0.9 vs. 0.7 ± 0.9, p = 0.0008; Stage 4: 3.3 ± 1.5 vs. 2.3 ± 1.7, p < 0.0001). Greater symptom burden at diagnosis was associated with worse survival in both EoCRC (p < 0.0001) and LoCRC (p < 0.0001). When controlling for cancer stage, both age (HR 0.87, 95% CI 0.8–1.0, p = 0.008) and increasing symptom number were independently associated with worse survival in multivariate models. Conclusions: Patients with EoCRC present with a greater number of symptoms of longer duration than LoCRC; however, time from patient reported symptom onset was not associated with worse outcomes.
The Evaluation and Management of Lung Metastases in Patients with Giant Cell Tumors of Bone in the Denosumab Era
Authors: Trovarelli Giulia , Rizzo Arianna , Cerchiaro Mariachiara , Pala Elisa , Angelini Andrea , Ruggieri Pietro
Published: April 2024

Giant cell tumor of bone (GCTB) is characterized by uncertain biological behavior due to its local aggressiveness and metastasizing potential. In this study, we conducted a meta-analysis of the contemporary literature to evaluate all management strategies for GCTB metastases. A combination of the terms “lung metastases”, “giant cell tumor”, “bone”, “treatment”, and “oncologic outcomes” returned 133 patients meeting our inclusion criteria: 64 males and 69 females, with a median age of 28 years (7–63), at the onset of primary GCTB. Lung metastases typically occur at a mean interval of 26 months (range: 0–143 months) after treatment of the primary site, commonly presenting as multiple and bilateral lesions. Various treatment approaches, including surgery, chemotherapy, radiotherapy, and drug administration, were employed, while 35 patients underwent routine monitoring only. Upon a mean follow-up of about 7 years (range: 1–32 years), 90% of patients were found to be alive, while 10% had died. Death occurred in 25% of patients who had chemotherapy, whereas 96% of those not treated or treated with Denosumab alone were alive at a mean follow-up of 6 years (range: 1–19 years). Given the typically favorable prognosis of lung metastases in patients with GCTB, additional interventions beyond a histological diagnosis confirmation may not be needed. Denosumab, by reducing the progression of the disease, can play a pivotal role in averting or delaying lung failure.
Real-World Treatment Patterns and Clinical Outcomes among Patients Receiving CDK4/6 Inhibitors for Metastatic Breast Cancer in a Canadian Setting Using AI-Extracted Data
Authors: Moulson Ruth , Feugère Guillaume , Moreira-Lucas Tracy S. , Dequen Florence , Weiss Jessica , Smith Janet , Brezden-Masley Christine
Published: April 2024

Cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) are widely used in patients with hormone receptor-positive (HR+)/human epidermal growth factor receptor 2 negative (HER2−) advanced/metastatic breast cancer (ABC/MBC) in first line (1L), but little is known about their real-world use and clinical outcomes long-term, in Canada. This study used Pentavere’s previously validated artificial intelligence (AI) to extract real-world data on the treatment patterns and outcomes of patients receiving CDK4/6i+endocrine therapy (ET) for HR+/HER2− ABC/MBC at Sinai Health in Toronto, Canada. Between 1 January 2016 and 1 July 2021, 48 patients were diagnosed with HR+/HER2− ABC/MBC and received CDK4/6i + ET. A total of 38 out of 48 patients received CDK4/6i + ET in 1L, of which 34 of the 38 (89.5%) received palbociclib + ET. In 2L, 12 of the 21 (57.1%) patients received CDK4/6i + ET, of which 58.3% received abemaciclib. In 3L, most patients received chemotherapy (10/12, 83.3%). For the patients receiving CDK4/6i in 1L, the median (95% CI) time to the next treatment was 42.3 (41.2, NA) months. The median (95% CI) time to chemotherapy was 46.5 (41.4, NA) months. The two-year overall survival (95% CI) was 97.4% (92.4, 100.0), and the median (range) follow-up was 28.7 (3.4–67.6) months. Despite the limitations inherent in real-world studies and a limited number of patients, these AI-extracted data complement previous studies, demonstrating the effectiveness of CDK4/6i + ET in the Canadian real-world 1L, with most patients receiving palbociclib as CDK4/6i in 1L.
Dissimilarity among Species and Higher Taxa of Amphibians in a Hotspot of Biodiversity and Endemism in the Neotropics
Authors: Calderón-Patrón Jaime Manuel , Peña-Joya Karen Elizabeth , Téllez-López Jorge , Canales-Gómez Eréndira Patricia
Published: April 2024

The Mexican Republic ranks fifth in the world in terms of amphibian diversity, and within Mexico, the state of Oaxaca has the greatest amphibian richness and endemism. Unfortunately, various factors, such as land use change and global warming, have caused a global crisis that threatens the conservation of this class. In the face of these threats, an analysis of beta diversity provides information that can be applied to conservation strategies, since its study reveals the spatial scaling of diversity loss and clarifies the mechanisms of regional diversity maintenance. In this work, we analyzed the beta diversity at the species and higher taxa level (order, family, subfamily, genus and species) for the amphibians of Oaxaca and their replacement components and the differences in richness for anurans and caudates separately between physiographic subprovinces. Very high beta diversity was recorded, with higher diversity occurring among caudates (0.92) than among anurans (0.84). Species replacement was the component that most contributed to this result, and the subprovinces with substantial environmental differences had the most dissimilar amphibian communities. The results of this study show the need to implement conservation strategies in subprovinces with high amphibian richness and endemism levels, following the example of Sierra Madre de Oaxaca (SMO), where local communities have developed conservation actions in most of the territory.