bluuebunny/crossref_metadata_2025 huggingface dataset:
Do disasters trigger protests? A conceptual view of the connection between disasters, injustice, and protests—The case of COVID-19
Authors: Vestergren Sara , Uysal Mete Sefa , Tekin Selin
Published: August 2022

People around the globe are affected by disasters far beyond the disaster properties. Given that certain social groups are affected disproportionately, disasters need to be considered as political events which may cause political actions. Therefore, we aim to discuss, from a social psychological perspective, how and why protests might occur during or after a disaster. We argue for an elaborated model of collective action participation suggesting that disasters enhance the predictors of protest mobilization and participation though emerged or enhanced social injustice. We also suggest that disaster properties can be used to delegitimise protests and social movements, limiting the mobilization and collective resilience during and after a disaster. Finally, we discuss the gaps in current research and emphasize the need for more attention to the disaster-protest link as we can expect more disasters due to climate crisis, likely to lead to more protests and political collective action.
Measuring students’ learning progressions in energy using cognitive diagnostic models
Authors: Zhou Shuqi , Traynor Anne
Published: August 2022

This study applied cognitive diagnostic models to assess students’ learning progressions in energy. A Q-matrix (i.e., an item attribute alignment table) was proposed based on existing literature about learning progressions of energy in the physical science domain and the Trends in International Mathematics and Science Study (TIMSS) assessment framework. The Q-matrix was validated by expert review and real data analysis. Then, the deterministic inputs, noisy ‘and’ gate (DINA) model with hierarchical relations was applied to data from three jurisdictions that had stable, defined science curricula (i.e., Australia, Hong Kong, and Ontario). The results suggested that the hypothesized learning progression was consistent with the observed progression in understanding the energy concept. We also found similarities in students’ attribute mastery across the three jurisdictions. In addition, we examined the instructional sensitivity of the selected item. We discuss several curriculum-related issues and student misconceptions that may affect students’ learning progressions and mastery patterns in different regions of the world.
COVID-19 in the United States as affective frame
Authors: Protevi John
Published: August 2022

In this paper I attempt to contribute to the developing field of “political philosophy of mind.” To render concrete the notion of “affective frame,” a social situation which pre-selects for salience and valence of environmental factors relative to a subject’s life, I conduct a case study of a deleterious socially instituted affective frame, which, during the early days of the COVID-19 pandemic in the United States, produced individuated circumstances that came crashing down on “essential workers” who were forced into a double bind. We saw here an untenable and ultimately fatal situation that forced a choice between, on the one hand, increasing the risk of their failing to provide financial support for their family if they quit their job or reduced their hours, and on the other, increasing their risk of contracting the virus by continuing to work. The case study will thus be itself an affective frame that will bring to the fore for its readers a nexus of harmful social practices of contemporary American society. Form is reinforced by content here, as this particular affective frame brings forth a further emphasis on affect when we focus on workers simultaneously socialized into roles as breadwinners and as members of the caring professions. For those people, quitting work becomes even more difficult as they come to affirm their self-identity of being providers of affective labor for those in their care at work and of being the affective anchor of family life at home, the one who financially helps keep a roof over the heads of their loved ones as well as being the emotional backbone of the family. Hence the affective frame of “essential workers in Covid times” renders salient and affirmatively valenced their affectively laden self-image as caring helpers of those in need, at home and at work.
Fostering Academic Engagement in Post-graduate Students: Assessing the Role of Positive Emotions, Positive Psychology, and Stress
Authors: Saleem Muhammad Shoaib , Isha Ahmad Shahrul Nizam , Awan Maheen Iqbal , Yusop Yuzana Binti , Naji Gehad Mohammed Ahmed
Published: August 2022

Aim

The current study attempted to assess the effect of positive emotion on post-graduate students’ psychological capital (PsyCap) as well as on their academic engagement behavior. Also, the direct relationship between PsyCap and academic engagement behavior was assessed alongside the presence of Stress as a moderating variable between PsyCap and academic engagement behavior amongst post-graduate students in Malaysia.

Materials and Methods

A self-administered questionnaire was used for data collection from 373 post-graduate Ph.D. students registered in various universities throughout Malaysia with a non-probabilistic sampling technique. Research respondents belonged to management, humanities, engineering, computer science, and health sciences domains, and they responded through a questionnaire copy. Statistically, structural equation modeling (SEM) was applied to evaluate confirmatory factor analysis (CFA), reliability analysis, validity analysis, measurement model, structural model, and path analysis. Furthermore, the (5000) bootstrapping approach was utilized to test the final model.

Results

For the hypothesized model, our results confirmed that positive emotions had a positive and significant effect on students’ psychological capita as well as on their academic engagement behavior. Further, PsyCap also had a positive and significant effect on academic engagement behavior. Our results also reported that stress as a moderating variable has a negative and deteriorating effect on the relationship between PsyCap and the academic engagement of students.

Conclusion

The study’s findings support the theoretical assumption that positive emotions help individuals generate cognitive resources, which in turn help them manage their engagement behavioral requirements. However, the stress caused by their study needs may deplete their psychological resources, consequently influencing their academic engagement behavior. Interventions like personal coaching/counseling, appropriate follow-up, and flexible goal settings with other measures may help post-graduate students in achieving their daunting tasks.
Role of social capital in adolescents’ online gaming: A longitudinal study focused on the moderating effect of social capital between gaming time and psychosocial factors
Authors: Kim Gyoung Mo , Jeong Eui Jun , Lee Ji Young , Yoo Ji Hye
Published: August 2022

Adolescents often create social relationships with their gaming peers who take on the role of offline friends and peer groups. Through collaboration and competition in the games, the social relationships of adolescents are becoming broader and thicker. Although this is a common phenomenon in online games, few studies have focused on the formation and roles of social capital among adolescent gamers. In particular, longitudinal research that examines the role of social capital in terms of influencing gaming time on adolescent gamers’ psychosocial factors has been minimal. This study was designed to fill this gap to see the long-term effect of social capital among adolescent gamers. Specifically, by using the three-year longitudinal data involving 403 adolescents, we analyzed the effect of gaming time on psychological factors (i.e., loneliness, depression, self-esteem, and life satisfaction) with the moderating role of social capital. Results showed that social capital played a crucial moderating role. In the higher social capital group, gaming time enhanced the degree of self-esteem and life satisfaction. However, a vicious circle was found in the lower social capital group: Gaming time increased the degree of depression but decreased self-esteem, which in turn led to increase in gaming time. These results indicate that games work as an important tool for social capital cultivation among adolescent gamers, which imply successful cultivation of social capital is a key to positive gaming effects. Theoretical and practical implications are discussed.
A study of the impacts of motivational regulation and self-regulated second-language writing strategies on college students’ proximal and distal writing enjoyment and anxiety
Authors: Zhang Yining , Dong Lianqi
Published: August 2022

Motivational regulation is crucial to explaining autonomous self-regulated learning, yet has received relatively little empirical attention. This study therefore examined how 230 college students’ motivational-regulation strategies affected their proximal and distal second-language writing-achievement emotions (i.e., enjoyment and anxiety), and sought evidence of interactive effects of such strategies and self-regulated learning strategies on each of these two types of emotions. All the studied types of motivational-regulation strategy were found to directly predict both proximal and distal writing enjoyment, under a “the more the happier” principle, but only a performance-oriented motivational regulation strategy predicted proximal or distal writing anxiety. A social-behavior learning strategy was found to counteract the high proximal anxiety caused by heavy use of the performance self-talk motivational regulation strategy; and motivational-regulation predictors also emerged as stable predictors of both proximal and distal writing well-being. These findings are expected to be both theoretically valuable to the study of motivational regulation under the self-regulated learning framework, and of practical value to educators, learners, and curriculum designers.
The effects of expectancies and patriotism on Chinese use intention of 5G network
Authors: Ke Zhang , Jiayi Hou , Long Chen
Published: August 2022

China leads the world in the development and rollout of 5G network, yet less research has been done on the drivers of Chinese people’s adoption of 5G network, especially the specific role of national sentiments, such as the influence of patriotism on their attitude toward 5G network. The study obtained 804 effective online questionnaires from the respondents of various ages, genders, areas, and educational levels. The results based on the structural equation modeling (SEM) analysis showed that patriotism was an antecedent to Chinese users’ cognition of the conditions provided by the government and telecom operators and the extent that users were influenced by media and surrounding people, which in turn increased users’ expectations of the performance of 5G network and their confidence in adapting to 5G network, and resulted in an increased willingness to use 5G network. This research helps to understand the role of patriotism, which is an emotional factor, in stimulating Chinese users’ attitudes toward 5G network at the time when 5G network is putting into large-scale commercial use in China.
Combined balance and plyometric training enhances knee function, but not proprioception of elite male badminton players: A pilot randomized controlled study
Authors: Zhou Limingfei , Gong Wangcheng , Wang Shixian , Guo Zhenxiang , Liu Meng , Chuang Samuel , Bao Dapeng , Zhou Junhong
Published: August 2022

Objectives

To investigate the effect of combined balance and plyometric training on knee function and proprioception of elite badminton athletes.

Methods

Sixteen elite male badminton players (age: 20.5 ± 1.1 years, height: 177.8 ± 5.1 cm, weight: 68.1 ± 7.2 kg, and training experience: 11.4 ± 1.4 years) volunteered to participate and were randomly assigned to a combined balance and plyometric training (CT) (n = 8) and plyometric (PT) group (n = 8). The CT group performed balance combined with plyometric training three times a week over 6 weeks (40 min of plyometrics and 20 min of balance training); while the PT group undertook only plyometric training for the same period (3–4 sets × 8–12 reps for each exercise). Both groups had the same technical training of badminton.

Results

The knee function and proprioception were assessed at baseline and after the intervention by measuring the performance of single-legged hop tests (LSIO, LSIT, LSIC, LSIS), standing postural sway (COPAP, COPML), and LSI of dominant leg and non-dominant leg. The results showed that as compared to PT, CT induced significantly greater improvements in LSIT and LSIS (p < 0.001) and significant greater percent increase in NAP (p = 0.011). The changes in LSIO, LSIC, DAP, NAP, LSIAP, DML, NML, and LSIML induced by CT did not differ from that induced by PT (p > 0.213).

Conclusion

In elite badminton players, intervention using CT holds great promise to augment the benefits for knee function compared to the intervention using PT only, and at the same time, with at least comparable benefits for proprioception. Future studies are needed to examine and confirm the results of this study.
Quality of life changes and their predictors in young adult narcolepsy patients after treatment: A real-world cohort study
Authors: Chin Wei-Chih , Wang Chih-Huan , Huang Yu-Shu , Hsu Jen-Fu , Chu Kuo-Chung , Tang I , Paiva Teresa
Published: August 2022

Background

We conducted a five-year prospective follow-up study to track the real-world quality of life of patients with narcolepsy after medication and analyzed predictors.

Methods

The study ultimately included 157 participants who completed 5-year follow-up, 111 had type 1 narcolepsy (NT1) and 46 had type 2 narcolepsy (NT2). Polysomnography, multiple sleep latency test, actigraphy and HLA-typing were conducted. The Short Form 36 Health Survey Questionnaire (SF-36), the Stanford Center for Narcolepsy Sleep Inventory, the Epworth Sleepiness Scale (ESS), the visual analog for hypersomnolence (VAS), and Conners' Continuous Performance Test were used. Descriptive statistics, repeated measures, and hierarchical linear models were applied for analysis.

Results

Most demographic and clinical data did not significantly differ between groups, but the NT1 group had significantly more overweight, more severe narcoleptic symptoms, more positive HLA typing, shorter mean sleep latency, and more sleep onset rapid eye movement periods. No significant change to the physical domains of SF-36 was found in the total group, but we observed significant changes in emotional role functioning and social function. The NT1 group showed significant improvements in physical role functioning, emotional role functioning, and social function. The NT2 group demonstrated significant improvements in emotional role functioning. At the baseline, the NT2 group had significantly better scores, but there was no significant group difference after treatment, except for physical and social function. ESS and VAS were significantly improved during follow-up. At the baseline, the NT1 group had significantly higher ESS and VAS scores, and continuously significantly higher ESS scores during follow-up. Narcolepsy types, HLA typing, age of onset, symptom severity, attention and vigilance were significantly correlated with SF-36.

Conclusion

Symptom control greatly associates with the quality of life in narcoleptic patients, and medication can play the most important role. Management targeting narcoleptic symptoms, attention impairment, and drug adherence should be provided.
The association of COVID-19 vaccine availability with mental health among adults in the United States
Authors: Shen Chan , Rashiwala Lucy , Wiener R. Constance , Findley Patricia A. , Wang Hao , Sambamoorthi Usha
Published: August 2022

Objective

To assess whether COVID-19 vaccine approval and availability was associated with reduction in the prevalence of depression and anxiety among adults in the United States.

Methods

We adopted cross sectional and quasi-experimental design with mental health measurements before vaccine availability (June 2020, N = 68,009) and after vaccine availability (March 2021, N = 63,932) using data from Census Pulse Survey. Depression and anxiety were derived from PHQ-2 and GAD-2 questionnaires. We compared rates of depression and anxiety between June 2020 and March 2021. Unadjusted and adjusted analysis with replicate weights were conducted.

Results

Depression prevalence was 25.0% in June 2020 and 24.6% in March 2021; anxiety prevalence was 31.7% in June 2020 and 30.0% in March 2021 in the sample. In adjusted analysis, there were no significant differences in likelihood of depression and anxiety between June 2020 and March 2021.

Conclusion

Depression and anxiety were not significantly different between June 2020 and March 2021, which suggests that the pandemic effect continues to persist even with widespread availability of vaccines.
Association between gestational depression and weight management behaviors during pregnancy: A cross-sectional study in Eastern China
Authors: Zhao Shiqi , Peng Xueqing , Zhou Hua , Ge Jinjin , Zhou Meng , Walker Anita Nyarkoa , You Hua
Published: August 2022

An undesirable psychological state may deteriorate individual's weight management-related behaviors. This study aims to see if ineffective weight control measures were linked to depressive symptoms during pregnancy. We conducted a cross-sectional questionnaire survey of 784 pregnant women and collected information on sociodemographic factors, maternal characteristics, depression, and weight management activities throughout pregnancy (exercise management, dietary management, self-monitoring regulation, and management objectives). About 17.5% of pregnant women exhibited depressive symptoms. The mean score on dietary management was upper-middle, exercise management and self-monitoring regulation were medium, and management objectives were lower-middle. Multivariable linear regression analysis revealed that pregnant women with depressive symptoms had lower levels of exercise management (β = −1.585, p = 0.005), dietary management (adjusted β = −0.984, p = 0.002), and management objectives (adjusted β = −0.726, p = 0.009). However, there was no significant relationship between depressive symptoms and pregnant women's self-monitoring regulating behavior (p > 0.05). The findings indicated the inverse association between depressive symptoms and gestational weight management behaviors. These results offer important indications for pregnancy weight management professionals by highlighting the need for mental health interventions for pregnant women experiencing depressive symptoms.
Activity system, schizotypal personality, and mentalization: A study between halted activity and COVID-19 conducted in Henan, China
Authors: El Maouch Mohamad , Wang Yile , Jin Zheng , Tamunang Tamutana Timothy , Zhao Kaibin , Liu Yu
Published: August 2022

The pandemic-related lifestyle has potentially imposed crucial disturbances on daily and long-term activities, which, in turn, were associated with thought disturbance. This study investigates how the characteristics of the activity system during pandemic-related restrictions are associated with other psychomental aspects. By focusing on PTSD, mentalization, and schizotypal personality, and by inquiring about the main components of the activity system of 852 college students (Zhengzhou, Henan, China)- including the goals orienting their activity, goals' terms and types, the motivation levels and sources, the activity type and engagement time, the flow of the activity, and how due to pandemic lifestyle-results revealed that the activity system's components have significant associations with PTSD, reflective function, and schizotypal traits. Additionally, some of the activity system's elements have a significant predictive role regarding schizotypal traits. The study considered that the life narrative during the pandemic has been disturbed; hence, this may have a crucial effect on mind coherence. Additionally, the outcomes from the pandemic context will support mental health interventions in other similar contexts where the life narrative is severely affected.
Menstrual cycles during COVID-19 lockdowns: A systematic review and meta-analysis
Authors: Chao Melissa , Menon Carlo , Elgendi Mohamed
Published: August 2022

Coronavirus disease 2019 lockdowns produced psychological and lifestyle consequences for women of reproductive age and changes in their menstrual cycles. To our knowledge, this is the first systematic review to characterize changes in menstrual cycle length associated with lockdowns compared to non-lockdown periods. A search on 5 May 2022 retrieved articles published between 1 December 2019, and 1 May 2022, from Medline, Embase, and Web of Science. The included articles were peer-reviewed observational studies with full texts in English, that reported menstrual cycle lengths during lockdowns and non-lockdowns. Cross-sectional and cohort studies were appraised using the Appraisal tool for Cross-Sectional Studies and the Cochrane Risk of Bias Tool for Cohort Studies, respectively. Review Manager was used to generate a forest plot with odds ratios (OR) at the 95% confidence interval (CI), finding a significant association between lockdown and menstrual cycle length changes for 21,729 women of reproductive age (OR = 9.14, CI: 3.16–26.50) with a significant overall effect of the mean (Z = 4.08, p < 0.0001). High heterogeneity with significant dispersion of values was observed (I2 = 99%, τ = 1.40, χ2 = 583.78, p < 0.0001). This review was limited by the availability of published articles that favored high-income countries. The results have implications for adequately preparing women and assisting them with menstrual concerns during lockdown periods.
The pattern of lymph node metastasis in peripheral pulmonary nodules patients and risk prediction models
Authors: Ke Lei , Ma Honghai , Zhang Qingyi , Wang Yiqing , Xia Pinghui , Yu Li , Lv Wang , Hu Jian
Published: August 2022

Background

For peripheral pulmonary nodules, the regularity of lymph node (LN) metastasis has not been studied. This study aimed to evaluate the metastasis pattern of intrapulmonary and relevant mediastinal lymph nodes in early-stage lung cancer, and further selected patients who were of low risk of LN metastasis as potential population to receive sub-lobectomy.

Methods

This study prospectively included consecutive patients with peripheral clinical T1N0M0 disease who underwent complete resection with LN dissection or sampling from August 2014 to July 2015. The patients were followed up to 15, May 2021. Univariable or multivariable Logistic analysis was used to identify the risk factors. Models predicting LN metastasis risk were conducted. The area under the curve for the receiver operating characteristic curves was used to evaluate the diagnostic value. Disease-free survival and overall survival were compared between groups.

Results

Finally, 201 patients were included in this study. For patients with negative tumor-bearing (TB) 13 and 14 station LNs, the positive rate of other lymph node stations was extremely low. Maximum CT value, pleural indentation and CEA level were risk factors for N1 station LNs metastasis. Besides, the factors above and lobulation sign were risk factors for skip metastasis beyond TB 13 and 14 station LNs. We constructed two scoring tables to predict N1 station metastasis and skip metastasis beyond TB 13 and 14 station. The AUC were 0·837 and 0·823, respectively. Based on the first table, 40·9% of patients suffered N1 station LNs metastasis and 27·3% had N2 disease in “high risk group” while the proportion was only 5·7% and 4·5% in “low risk group”. For patients with negative TB13 and TB14 station LNs, based on the latter table, 11·1% of patients had N1 stations LNs metastasis and 16·7% had pN2 disease in “high risk group” while only 2·3% patients in “low risk group” suffered this kind of metastasis.

Conclusion

For peripheral pulmonary nodules patients, stations 13 and 14 LNs may be the sentinel nodes. For patients with low risk of N1 metastasis and skip metastasis, sub-lobar resection might be sufficient for those who were of negative TB 13 and 14 station LNs.
The role of postoperative radiotherapy in patients with uterine sarcomas: A PSM-IPTW analysis based on SEER database
Authors: Hao Zhimin , Yang Sufen
Published: August 2022

Objective

The optimal adjuvant therapy for uterine sarcomas remains poorly determined due to its rarity and histological diversity. The purpose of the study is to explore and characterize the association between utilization of radiotherapy and survival outcome in patients with surgically resected uterine sarcomas.

Methods

We collected data regarding uterine sarcomas which were confirmed after total hysterectomy between 2010 and 2018 period from the latest version of the Surveillance, Epidemiology, and End Results (SEER) database. Initially, 1-, 3- and 5-year survival rate were calculated to predict potential risk factors and possible role of adjuvant chemotherapy and radiotherapy. Propensity score matching (PSM) and inverse probability treatment weighting (IPTW) technique were employed to balance confounding factors in the utilization of additional therapy. Multivariate and exploratory subgroup analyses were respectively conducted to evaluate the impact of adjuvant therapy on overall survival (OS) and cause-specific survival (CSS).

Results

A total of 2897 patients were enrolled in the analysis. Survival benefit at 1-, 3-and 5-year after initial treatment was observed in the group of radiotherapy given, however, poorer prognosis in the group of chemotherapy administration. Accordingly, chemotherapy was enrolled as a confounding factor when stratifying and matching patients by receipt of radiotherapy. Prior to and after PSM-IPTW adjustment, radiotherapy both demonstrated beneficial effect on OS and CSS based on multivariate analysis. Further subgroup analysis indicated radiotherapy improved OS and CSS among a subset of patients in stage II-IV, particularly with uterine leiomyosarcoma, tumor grade IV, bigger tumor size than 100 mm and even with chemotherapy administration.

Conclusions

Adjuvant radiotherapy in uterine sarcomas after hysterectomy might be underutilized, and proper use of adjuvant radiotherapy combined with chemotherapy after surgery in advanced-stage and high-risk patients might improve survival.
Thermography for disease detection in livestock: A scoping review
Authors: McManus Rosemary , Boden Lisa A. , Weir William , Viora Lorenzo , Barker Robert , Kim Yunhyong , McBride Pauline , Yang Shufan
Published: August 2022

Infra-red thermography (IRT) offers potential opportunities as a tool for disease detection in livestock. Despite considerable research in this area, there are no common standards or protocols for managing IRT parameters in animal disease detection research. In this review, we investigate parameters that are essential to the progression of this tool and make recommendations for their use based on the literature found and the veterinary thermography guidelines from the American Academy of Thermology. We analyzed a defined set of 109 articles concerned with the use of IRT in livestock related to disease and from these articles, parameters for accurate IRT were identified and sorted into the fields of camera-, animal- or environment-related categories to assess the practices of each article in reporting parameters. This review demonstrates the inconsistencies in practice across peer-reviewed articles and reveals that some important parameters are completely unreported while others are incorrectly captured and/or under-represented in the literature. Further to this, our review highlights the lack of measured emissivity values for live animals in multiple species. We present guidelines for the standards of parameters that should be used and reported in future experiments and discuss potential opportunities and challenges associated with using IRT for disease detection in livestock.
COVID-19 Information-Seeking, Health Literacy, and Worry and Anxiety During the Early Stage of the Pandemic in Switzerland: A Cross-Sectional Study
Authors: Ilic Anica , Roser Katharina , Sommer Grit , Baenziger Julia , Mitter Vera Ruth , Mader Luzius , Dyntar Daniela , Michel Gisela
Published: August 2022

Objectives: To describe COVID-19 information-seeking behavior (CISB) during the first stage of the pandemic in Switzerland and identify its determinants.

Methods: We conducted an online cross-sectional survey (4 May to 6 July 2020). Participants self-reported their CISB (information sources and frequency), personal COVID-19 situation (e.g., perception about having had COVID-19), sociodemographic information, and completed validated measures of health literacy, and worry and anxiety. Data were analyzed using descriptive statistics and logistic regressions.

Results: We included 1,505 participants (24.7% male; mean age = 43.0 years, SD = 13.9). Most participants reported searching for information daily (n = 1,023, 68.0%) and referring to multiple information sources (mean 3.7, SD = 1.5). Commonly used sources were official websites (n = 1,129, 75.0%) and newspapers (n = 997, 66.2%). Participants with higher health literacy were more likely to seek information daily and use online resources, but less likely to use personal networks than those with lower health literacy. We did not find any association between CISB and worry and anxiety.

Conclusion: More opportunities for personal dialogue and education about reliable online information resources should be encouraged to optimize the CISB of groups with lower health literacy.
Dielectric Elastomer-Driven Bionic Inchworm Soft Robot Realizes Forward and Backward Movement and Jump
Authors: Jing Zeying , Li Qingzhong , Su Wentai , Chen Yuan
Published: August 2022

To produce multi-modal mobility in complicated situations is a significant issue for soft robots. In this study, we show the conception, construction, and operation of an inchworm-impersonating dielectric elastomer-activated soft robot. The robot is small and lightweight, weighing only 3.5 g, and measuring an overall 110 mm by 50 mm by 60 mm (length, width, and height). The three mobility modes for the robot are each equipped with a detailed mechanism. When the excitation voltage is 5 kV, the robot runs forward under a frequency of stimulation of 1–9 Hz, and its direction of motion changes to a backwards motion at >10 Hz. When the excitation voltage of 5.5 kV is applied to the robot, the robot runs forward at 1–12 Hz frequency and moves in the opposite direction at 13 Hz, reaching the fastest reverse speed of 240 mm/s. When the excitation voltage rises to 6 kV, the robot reaches its fastest running speed of 270 mm/s at 14 Hz. Motivated by high voltage and high duty cycle, the robot can jump over obstacles of 5 mm. In order to assess the performance of backward running, the speed achieved by the robot under a 30% duty cycle and a 50% duty cycle was compared, as well as the speed of the robot with or without the use of a counterweight. The robot has a simpler design and construction than earlier soft robots of the same kind, as well as a quicker speed, a wider variety of movement modes, and other notable advantages.
3DPhenoMVS: A Low-Cost 3D Tomato Phenotyping Pipeline Using 3D Reconstruction Point Cloud Based on Multiview Images
Authors: Wang Yinghua , Hu Songtao , Ren He , Yang Wanneng , Zhai Ruifang
Published: August 2022

Manual phenotyping of tomato plants is time consuming and labor intensive. Due to the lack of low-cost and open-access 3D phenotyping tools, the dynamic 3D growth of tomato plants during all growth stages has not been fully explored. In this study, based on the 3D structural data points generated by employing structures from motion algorithms on multiple-view images, we proposed a 3D phenotyping pipeline, 3DPhenoMVS, to calculate 17 phenotypic traits of tomato plants covering the whole life cycle. Among all the phenotypic traits, six of them were used for accuracy evaluation because the true values can be generated by manual measurements, and the results showed that the R2 values between the phenotypic traits and the manual ones ranged from 0.72 to 0.97. In addition, to investigate the environmental influence on tomato plant growth and yield in the greenhouse, eight tomato plants were chosen and phenotyped during seven growth stages according to different light intensities, temperatures, and humidities. The results showed that stronger light intensity and moderate temperature and humidity contribute to a higher biomass and higher yield. In conclusion, we developed a low-cost and open-access 3D phenotyping pipeline for tomato and other plants, and the generalization test was also complemented on other six species, which demonstrated that the proposed pipeline will benefit plant breeding, cultivation research, and functional genomics in the future.
Transcriptome and Re-Sequencing Analyses Reveal Photosynthesis-Related Genes Involvement in Lutein Accumulation in Yellow Taproot Mutants of Carrot
Authors: Wu Zhe , Xu Hui , Yang Xuan , Li Lixia , Luo Dan , Liu Zhenzhen , Jia Li
Published: August 2022

Carrots accumulate numerous carotenoids in the root, resulting in different colors. Orange carrots are primarily high in α- and β-carotene, while yellow carrots are packed with lutein. This study was designed to explore the molecular mechanism underlying the yellow mutation involving lutein using a recently obtained yellow root mutant carrot (ym) via mutagenesis of an orange root wild type (wt). Microscopes were used to observe the variations in histological and cellular structures, and transcriptome and resequencing analyses were conducted for ym and wt. The root callus of ym contained fewer colored crystals and globular chromoplasts than those of wt. Based on ribonucleic acid sequencing (RNA-seq) data analysis, 19 photosynthesis-related differentially expressed genes (DEGs) were enriched. Among them, there were 6 photosynthesis-related genes experiencing nonsynonymous mutations, including PSAL, PSB27-1, psbB, and three homologs of LHCB1.3, and Lut 5, the mapped gene regulating lutein content in carrot root, also had nonsynonymous mutations in ym. These 7 genes were shown to be significantly differently expressed at one or more time points during the lutein accumulation process. It is predicted that the 6 photosynthesis-related genes and Lut 5 are candidate genes for lutein accumulation, which results in root color mutation. The candidate genes identified in this study can provide a new insight into the molecular mechanism of lutein modulation.
Unravelling the Endophytic Virome Inhabiting Maize Plant
Authors: Fadiji Ayomide Emmanuel , Galeemelwe Onalenna , Babalola Olubukola Oluranti
Published: August 2022

Endophytes are well-known for their symbiotic interaction with plants and their ability to promote plant growth by producing various metabolites. The most well-studied endophytes are bacteria and fungi. For generations, viruses were misnamed, and their symbiotic associations were ambiguous. Recent advances in omics techniques, particularly next-generation sequencing, have given rise to novel developments in the mutualistic relationships that exist between plants and viruses. Endogenous viruses have received a lot of attention in the animal world, but limited information exists on their functions and importance to plants. Therefore, endophytic viral populations inhabiting the root of a maize plant were assessed in this study for the first time using shotgun metagenomics. Complete DNA was extracted and sequenced using shotgun metagenomics from the maize roots in farming sites where organic fertilization (FZ), inorganic fertilization (CZ), and maize planted with no fertilization (NZ) are being practised in an experimental field. Our results identified 2 orders namely: Caudovirales (67.5%) and Herpesvirales (28.5%) which dominated the FZ site, although they do not show any significant difference (p > 0.05) across the sites. At the class level Microviridae, Phycodnaviridae, Podoviridae, Phycodnaviridae, and Poxviridae dominated the FZ site. Myoviridae and Podoviridae were more abundant in the CZ site, while only Siphoviridae predominated the inorganic fertiliser site (NZ). Diversity analysis revealed that viral populations were more abundant in organic fertilization (FZ). Taken together, this research adds to our understanding of the symbiotic integration of endophytic viruses with maize plants and that their abundance is affected by farming practices. In addition, their potential can be exploited to solve a variety of agronomic issues.
Evaluation of Morpho-Physiological Traits in Rice Genotypes for Adaptation under Irrigated and Water-Limited Environments
Authors: Gaballah Mahmoud M. , Ghoneim Adel M. , Rehman Hafeez Ur , Shehab Mohamed M. , Ghazy Mohamed I. , El-Iraqi Ahmed S. , Mohamed Abdelwahed E. , Waqas Muhammad , Shamsudin Noraziyah Abd Aziz , Chen Yaning
Published: August 2022

Drought is one of the major limitations to rice productivity worldwide. The present study compared variation in seventeen rice genotypes of Egyptian origin for morpho-physiological traits to identify the best genotypes with combination of adaptive traits under water-limited condition (DS). The DS reduced days to heading (DTH), plant height (PH), flag leaf angle (FLA), flag leaf area (FLAR), chlorophyll content (CHC), relative water content (RWC), grain yield (GY), and its components. Among genotypes, Hybrid 2 expressed the highest GY, panicle length (PL), number of tillers (NT), panicles per plant (NPP), and harvest index (HI) with maximum spikelet sterility (SS) under non-stress condition (NS), while the same genotype expressed ≈ 41% yield reduction under DS. The genotype Giza 179 had earlier DTH, higher and stable GY, FLAR, and yield component traits such as NPP, PW, and HI across the water regimes with least yield reduction (30.5%) under DS. The GY and FLAR, RWC, PL, NT, NPP, PW, and HI were positively correlated under DS. The cluster analysis showed a similarity index of 25% among genotypes. The high yielding genotypes Giza 179, IET 1444, and IRAT 170 had also increased yield components (PL, NT, NPP, PW, TGW and HI) under DS that were attributed to highest FLAR, RWC, and PH, while having reduced LR, FLA, TR, and SS; therefore, these genotypes were categorized as drought-tolerant. The Hybrid 2 and Giza 179 genotypes can perform well under NS; however, the cultivation of Giza 179, Sakha 107, IET 1444, and IRAT 170 would give an advantage in DS-prone areas, hence, these can be used as a donor parental line in future rice breeding programs.
Soil Quality Index as Affected by Integrated Nutrient Management in the Himalayan Foothills
Authors: Shah Tajamul Islam , Shah Aanisa Manzoor , Bangroo Shabir Ahmed , Sharma Manbir Pal , Aezum Aziz Mujtaba , Kirmani Nayar Afaq , Lone Aabid Hussain , Jeelani Mohammad Iqbal , Rai Ajai Pratap , Wani Fehim Jeelani , Bhat Mohammad Iqbal , Malik Abdul Raouf , Biswas Asim , Ahmad Latief
Published: August 2022

Soil quality assessment serves as an index for appraising soil sustainability under varied soil management approaches. Our current investigation was oriented to establish a minimum data set (MDS) of soil quality indicators through the selection of apt scoring functions for each indicator, thus evaluating soil quality in the Himalayan foothills. The experiment was conducted during two consecutive years, viz. 2016 and 2017, and comprised of 13 treatments encompassing different combinations of chemical fertilizers, organic manure, and biofertilizers, viz. (i) the control, (ii) 20 kg P + PSB (Phosphorus solubilizing bacteria), (iii) 20 kg P + PSB + Rhizobium, (iv) 20 kg P + PSB + Rhizobium+ FYM, (v) 20 kg P + 0.5 kg Mo + PSB, (vi) 20 kg P + 0.5 kg Mo + PSB + Rhizobium, (vii) 20 kg P + 0.5 kg Mo + PSB + Rhizobium + FYM, (viii) 40 kg@ P + PSB, (ix) 40 kg P + PSB + Rhizobium, (x) 40 kg P + PSB + Rhizobium+ FYM, (xi) 40 kg P + 0.5 kg Mo + PSB, (xii) 40 kg P + 0.5 kg Mo + PSB + Rhizobium, and (xiii) 40 kg P + 0.5 kg Mo + PSB + Rhizobium + FYM. Evaluating the physical, chemical, and biological indicators, the integrated module of organic and inorganic fertilization reflected a significant improvement in soil characteristics such as the water holding capacity, available nitrogen, phosphorus, potassium, and molybdenum, different carbon fractions and soil biological characteristics encircling microbial biomass carbon (MBC), and total bacterial and fungal count. A principal component analysis (PCA) was executed for the reduction of multidimensional data ensued by scoring through the transformation of selected indicators. The soil quality index (SQI) established for different treatments exhibited a variation of 0.105 to 0.398, while the magnitude of share pertaining to key soil quality indicators for influencing soil quality index encircled the water holding capacity (WHC), the dehydrogenase activity (DHA), the total bacteria count, and the available P. The treatments that received an integrated nutrient package exhibited a higher SQI (T10—0.398; T13—0.372; T7—0.307) in comparison to the control treatment (T1—0.105). An enhanced soil quality index put forth for all organic treatments reflected an edge of any conjunctive package of reduced synthetic fertilizers with prime involvement of organic fertilizers over the sole application of inorganic fertilizers.
Comparison of Aboveground Vegetation and Soil Seed Bank Composition among Three Typical Vegetation Types in the Karst Regions of Southwest China
Authors: Guo Yili , Li Yufei , Li Jianxing , Li Jiaqi , Wen Shujun , Huang Fuzhao , He Wen , Wang Bin , Lu Shuhua , Li Dongxing , Xiang Wusheng , Li Xiankun
Published: August 2022

Rural agricultural activity generates cropland, secondary vegetation and straggling primary forest and can modify the soil seed bank (SSB), potentially impacting the restoration of preferred species. The interaction between vegetation and seed banks during the recovery process is dependent on management practices and recovery pathways. This study was carried out in Guilin of southwest China to assess the variation in plant diversity and species composition of both aboveground and soil seed banks across three typical vegetation types with different human interventions: orchard, bamboo shrub and primary forest. The results show that there were significant differences in the species composition and diversity of aboveground vegetation and SSB, as well as in soil properties among three typical vegetation types. The primary forest had the highest aboveground species diversity, while the orchard had the highest species diversity and seed density of SSB. In addition, principal component analysis (PCA) and canonical correspondence analyses (CCAs) showed that the species composition and plant life forms of the three typical vegetation types were significantly influenced by soil properties. Based on these findings, the characteristics of aboveground vegetation and the soil seed bank and their correlations with soil properties are expected to drastically change with human intervention. These results imply that unsustainable land use has greatly impacted soil properties, and consequently, the aboveground vegetation and SSB. Nevertheless, vegetation will recover quickly after farming is abandoned. The successful restoration of fragmented ecosystems requires the addition of seeds and seedlings of target species, especially perennial woody plants from the relevant natural ecosystems, to accelerate succession from bamboo shrub to forest.
Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection
Authors: Hu Xi , Jiang Nan , Li Yiqun , Zhou Yong , Fan Yuding , Xue Mingyang , Zeng Lingbing , Liu Wenzhi , Meng Yan
Published: August 2022

Molecular diagnostic testing for viral pathogens is crucial in aquaculture. The efficient and convenient preparation of pathogenic microbial nucleic acids is the basis of molecular diagnosis. Here, we developed a simplified deoxyribonucleic acid (DNA) extraction method from aquatic animal DNA viruses using the Chelex 100 resin. The nucleic acid was extracted from infected tissues and cell culture for the detection of three common aquatic viral pathogens (CEV, CyHV-2, and GSIV). We compared the extraction effects of a current commercial kit extraction method and the Chelex 100 resin extraction method according to nucleic acid concentration, conventional polymerase chain reaction (PCR), and digital droplet PCR (ddPCR). The results indicated that both extraction procedures could obtain high-quality nucleotide samples. Extracting DNA using the Chelex 100 resin led to better detective efficiency for ddPCR molecular diagnostic testing. The whole process took less than 20 min, and only Chelex 100 resin solution was added to the tissues or cells without multiple tubes being transferred several times. The extracted DNA concentration and the detection sensitivity were high. These results indicated that the Chelex 100 resin solution has the advantages of speed, efficiency, and economy compared to the commercial kit. In addition, the higher pH value (10–11) of the Chelex 100 resin solution markedly improved the detection sensitivity compared to a lower pH value (9–10). In conclusion, the comparison of the Chelex 100 Resin and commercial viral DNA extraction kits revealed the good performance of the Chelex 100 resin solution at pH 10–11 in DNA extraction for PCR amplification from aquatic animal viral samples of tissues and cells in molecular diagnostic testing. It is both rapid and cost-effective.
Accuracy to Predict the Onset of Calving in Dairy Farms by Using Different Precision Livestock Farming Devices
Authors: Szenci Ottó
Published: August 2022

Besides traditional methods such as evaluation of the external preparatory and behavioral signs, which even presently are widely used also in large dairy farms, there are several new possibilities such as measuring body (intravaginal, ventral tail-base surface, ear surface, or reticulo-ruminal) temperature, detecting behavioral signs (rumination, eating, activity, tail raising) or detecting the expulsion of the device inserted into the vagina or fixed to the skin of the vulva when allantochorion appears in the vulva to predict the onset of the second stage of calving. Presently none of the single sensors or a combination of sensors can predict the onset of calving with acceptable accuracy. At the same time, with the exception of the iVET® birth monitoring system, not only the imminent onset of calving could be predicted with high accuracy, but a significantly lower prevalence rate of dystocia, stillbirth, retained fetal membranes, uterine diseases/clinical metritis could be reached while calving-to-conception interval was significantly shorter compared with the control groups. These results may confirm the use of these devices in dairy farms by allowing appropriate intervention during calving when needed. In this way, we can reduce the negative effect of dystocia on calves and their dams and improve their welfare.