bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Chemistry Based Gold Nanoparticles Synthesis Using the Marine Bacterium Lysinibacillus odysseyi PBCW2 and Their Multitudinous Activities
Authors: Cherian Tijo , Maity Debasis , Rajendra Kumar Ramasamy T. , Balasubramani Govindasamy , Ragavendran Chinnasamy , Yalla Suneelkumar , Mohanraju Raju , Peijnenburg Willie J. G. M.
Published: August 2022

Green chemistry has paved an ‘avant-garde avenue’ in the production and fabrication of eco-friendly stable nanoparticles employing the utilization of biological agents. In the present study we present the first report on the potential of the marine bacterium Lysinibacillus odysseyi PBCW2 for the extracellular production of gold nanoparticles (AuNPs). Utilizing a variety of methods, AuNPs in the cell-free supernatant of L. odysseyi (CFS-LBOE) were identified and their antioxidant, antibacterial, and dye-degrading properties were examined. The visual coloring of the reaction mixture to a ruby red hue showed the production of LBOE-AuNPs; validated by means of XRD, TEM, SEM, XRD, DLS, TGA, and FT-IR analysis. Additionally, the 2,2-diphenyl-1-picrylhydrazyl technique and the well diffusion assay were used to examine their dose-dependent antioxidant and antibacterial activity. These biogenic LBOE-AuNPs showed 91% dye degradation efficiency during catalytic reduction activity on BTB dye, demonstrating their versatility as options for heterogeneous catalysis.
Tackling Structural Complexity in Li2S-P2S5 Solid-State Electrolytes Using Machine Learning Potentials
Authors: Staacke Carsten G. , Huss Tabea , Margraf Johannes T. , Reuter Karsten , Scheurer Christoph
Published: August 2022

The lithium thiophosphate (LPS) material class provides promising candidates for solid-state electrolytes (SSEs) in lithium ion batteries due to high lithium ion conductivities, non-critical elements, and low material cost. LPS materials are characterized by complex thiophosphate microchemistry and structural disorder influencing the material performance. To overcome the length and time scale restrictions of ab initio calculations to industrially applicable LPS materials, we develop a near-universal machine-learning interatomic potential for the LPS material class. The trained Gaussian Approximation Potential (GAP) can likewise describe crystal and glassy materials and different P-S connectivities PmSn. We apply the GAP surrogate model to probe lithium ion conductivity and the influence of thiophosphate subunits on the latter. The materials studied are crystals (modifications of Li3PS4 and Li7P3S11), and glasses of the xLi2S–(100 – x)P2S5 type (x = 67, 70 and 75). The obtained material properties are well aligned with experimental findings and we underscore the role of anion dynamics on lithium ion conductivity in glassy LPS. The GAP surrogate approach allows for a variety of extensions and transferability to other SSEs.
Box–Behnken Design: Optimization of Proanthocyanidin-Loaded Transferosomes as an Effective Therapeutic Approach for Osteoarthritis
Authors: Tamilarasan Neelakandan , Yasmin Begum M. , Anitha Posina , Umme Hani , Cheng Wan Hee , Mohan Sellapan , Ramkanth Sundarapandian , Janakiraman Ashok Kumar
Published: August 2022

Transferosomes are one of the vesicular carriers that have received extensive research and attention recently because of their capacity to get beyond the barriers posed by the stratum corneum to penetration. The intent of the current study is to optimize and evaluate proanthocyanidin (PAC) containing transferosomal transdermal gels. PAC-containing transferosomes were prepared using the film hydration method and then loaded into a 4% methylcellulose gel. A 23 Box–Behnken design was used to optimize the PAC-loaded transferosomal gel, where the effects of phospholipid 90 G (X1), Tween 80 (X2), and sonication time (X3) were evaluated. The formulation factors, such as the drug entrapment efficiency percentage (PEE) and in vitro drug release, were characterized. A PEE of 78.29 ± 1.43% and a drug release in vitro at 6 h of 24.2 ± 1.25% were obtained. The optimized transferosomal-loaded proanthocyanidin (OTP) formulation penetrated the porcine skin at an excellent rate (0.123 ± 0.0067 mg/cm2/h). Stability tests were conducted for OTP to predict the effects of various temperature conditions on the physical appearance, drug content, and PEE for periods of 15, 30, and 45 days. Finally, this transferosomal system for transdermal PAC delivery may be a suitable alternative to the conventional treatment for osteoarthritis.
A Review of Performance Prediction Based on Machine Learning in Materials Science
Authors: Fu Ziyang , Liu Weiyi , Huang Chen , Mei Tao
Published: August 2022

With increasing demand in many areas, materials are constantly evolving. However, they still have numerous practical constraints. The rational design and discovery of new materials can create a huge technological and social impact. However, such rational design and discovery require a holistic, multi-stage design process, including the design of the material composition, material structure, material properties as well as process design and engineering. Such a complex exploration using traditional scientific methods is not only blind but also a huge waste of time and resources. Machine learning (ML), which is used across data to find correlations in material properties and understand the chemical properties of materials, is being considered a new way to explore the materials field. This paper reviews some of the major recent advances and applications of ML in the field of properties prediction of materials and discusses the key challenges and opportunities in this cross-cutting area.
Influence of Grain Boundary Scattering on the Field-Effect Mobility of Solid-Phase Crystallized Hydrogenated Polycrystalline In2O3 (In2O3:H)
Authors: Magari Yusaku , Yeh Wenchang , Ina Toshiaki , Furuta Mamoru
Published: August 2022

Hydrogenated polycrystalline In2O3 (In2O3:H) thin-film transistors (TFTs) fabricated via the low-temperature solid-phase crystallization (SPC) process with a field-effect mobility (μFE) exceeding 100 cm2 V−1 s−1 are promising candidates for future electronics applications. In this study, we investigated the effects of the SPC temperature of Ar + O2 + H2-sputtered In2O3:H films on the electron transport properties of In2O3:H TFTs. The In2O3:H TFT with an SPC temperature of 300 °C exhibited the best performance, having the largest µFE of 139.2 cm2 V−1 s−1. In contrast, the µFE was slightly degraded with increasing SPC temperature (400 °C and higher). Extended X-ray absorption fine structure analysis revealed that the medium-range ordering in the In2O3:H network was further improved by annealing up to 600 °C, while a large amount of H2O was desorbed from the In2O3:H films at SPC temperatures above 400 °C, resulting in the creation of defects at grain boundaries. The threshold temperature of H2O desorption corresponded well with the carrier transport properties; the µFE of the TFTs started to deteriorate at SPC temperatures of 400 °C and higher. Thus, it was suggested that the hydrogen remaining in the film after SPC plays an important role in the passivation of electron traps, especially for grain boundaries, resulting in an enhancement of the µFE of In2O3:H TFTs.
Recent Progress on Triboelectric Nanogenerators for Vibration Energy Harvesting and Vibration Sensing
Authors: Haroun Ahmed , Tarek Mohamed , Mosleh Mohamed , Ismail Farouk
Published: August 2022

The triboelectric nanogenerator (TENG) is a recent technology that reforms kinetic energy generation and motion sensing. A TENG comes with variety of structures and mechanisms that make it suitable for wide range of applications and working conditions. Since mechanical vibrations are abundant source of energy in the surrounding environment, the development of a TENG for vibration energy harvesting and vibration measurements has attracted a huge attention and great research interest through the past two decades. Due to the high output voltage and high-power density of a TENG, it can be used as a sustainable power supply for small electronics, smart devices, and wireless sensors. In addition, it can work as a vibration sensor with high sensitivity. This article reviews the recent progress in the development of a TENG for vibration energy harvesting and vibration measurements. Systems of only a TENG or a hybrid TENG with other transduction technologies, such as piezoelectric and electromagnetic, can be utilized for vibrations scavenging. Vibration measurement can be done by measuring either vibration displacement or vibration acceleration. Each can provide full information about the vibration amplitude and frequency. Some TENG vibration-sensing architectures may also be used for energy harvesting due to their large output power. Numerous applications can rely on TENG vibration sensors such as machine condition monitoring, structure health monitoring, and the Internet of things (IoT).
Analytical Model of CVD Growth of Graphene on Cu(111) Surface
Authors: Popov Ilya , Bügel Patrick , Kozlowska Mariana , Fink Karin , Studt Felix , Sharapa Dmitry I.
Published: August 2022

Although the CVD synthesis of graphene on Cu(111) is an industrial process of outstanding importance, its theoretical description and modeling are hampered by its multiscale nature and the large number of elementary reactions involved. In this work, we propose an analytical model of graphene nucleation and growth on Cu(111) surfaces based on the combination of kinetic nucleation theory and the DFT simulations of elementary steps. In the framework of the proposed model, the mechanism of graphene nucleation is analyzed with particular emphasis on the roles played by the two main feeding species, C and C2. Our analysis reveals unexpected patterns of graphene growth, not typical for classical nucleation theories. In addition, we show that the proposed theory allows for the reproduction of the experimentally observed characteristics of polycrystalline graphene samples in the most computationally efficient way.
Nematicidal Potential of Green Silver Nanoparticles Synthesized Using Aqueous Root Extract of Glycyrrhiza glabra
Authors: Rani Kanika , Devi Nisha , Banakar Prakash , Kharb Pushpa , Kaushik Prashant
Published: August 2022

Meloidogyne incognita (root-knot nematode) is a devastating soil-borne pathogen which can infect almost all cultivated plants around the globe, expediting huge pecuniary losses. The purpose of current study was to use the aqueous root extract of Glycyrrhiza glabra for synthesizing silver nanoparticles (GRAgNPs) and assess their nematicidal potential against M. incognita by in vitro methods, including hatching inhibition and mortality assays. The active uptake of FITC labeled GRAgNPs by the nematode and their effect on the expression of selected genes involved in oxidative stress and DNA damage repair were also studied. An HRTEM micrograph confirmed their spherical morphology with sizes ranging from 9.61 nm to 34.735 nm. Complete inhibition of egg-hatching was observed after 48 h of treatment with as low as 10.0 ppm of GRAgNPs. In addition, 100% mortality was recorded at the lowest dose of 6.0 ppm, after 12 h of treatment. The LC-50 for GRAgNPs was found to be 0.805 ± 0.177 ppm at p < 0.0001, R2 = 0.9930, and α = 0.05. The expression of targeted genes (skn-1, mev-1, sod-3, dhs-23, cyp-450, xpa, cpr-1, gst-n, and ugt) was significantly enhanced (1.09–2.79 folds), at 1.0 ppm (α = 0.05, 95% CI) GRAgNPs treatment. In conclusion, GRAgNPs performed efficaciously and considerably in contrast to chemical nematicide and commercial silver nanoparticles (CAgNPs) and might be used as a promising alternative as relatively lower concentration and short exposure time were enough to cause higher mortality and nanotoxicity in nematodes.
N-Doped Honeycomb-like Ag@N-Ti3C2Tx Foam for Electromagnetic Interference Shielding
Authors: Wang Xiaohan , Zhang Fan , Hu Feiyue , Li Yaya , Chen Yongqiang , Wang Hailong , Min Zhiyu , Zhang Rui
Published: August 2022

To solve the pollution problem of electromagnetic waves, new electromagnetic shielding materials should meet the requirements of being lightweight with high electrical conductivity. In this work, the combination of silver (Ag) nanoparticles and nitrogen doping (N-doping) was expected to tune the electromagnetic and physical properties of Ti3C2Tx MXene, and the Ag@N-Ti3C2Tx composites were fabricated through the hydrothermal reactions. The nitrogen doped (N-doped) Ag@Ti3C2Tx composites showed a hollow structure with a pore size of 5 μm. The influence of N-doped degrees on the electromagnetic interference (EMI) shielding performance was investigated over 8–18 GHz. Therefore, the controlled N-doping composites exhibited reflection-based EMI shielding performance due to the electrical conductivity and the special three-dimensional (3D) honeycomb-like structure. The achieved average EMI shielding values were 52.38 dB at the X-band and 72.72 dB at the Ku-band. Overall, the Ag@N-Ti3C2Tx foam, due to its special 3D honeycomb-like structure, not only meets the characteristics of light weight, but also exhibits ultra-high-efficiency EMI shielding performance, revealing great prospects in the application of electromagnetic wave shielding field.
Nanoencapsulation of Acetamiprid by Sodium Alginate and Polyethylene Glycol Enhanced Its Insecticidal Efficiency
Authors: Ebadollahi Asgar , Valizadeh Bita , Panahandeh Saleh , Mirhosseini Hadiseh , Zolfaghari Maryam , Changbunjong Tanasak
Published: August 2022

Nanoformulation has been considered one of the newly applied methods in integrated pest management strategies. In this research, a conventional neonicotinoid insecticide acetamiprid was nanoencapsulated via AL (Sodium Alginate) and PEG (Polyethylene Glycol) and tested against the elm leaf beetle Xanthogaleruca luteola. The synthesized particles had spherical-like morphology and nanoscale based on TEM (Transmission Electron Microscopy) and DLS (Dynamic Light Scattering). The encapsulation efficiency and loading percentages of acetamiprid in AL and PEG were 92.58% and 90.15%, and 88.46% and 86.79%, respectively. Leaf discs treated with different formulations by the leaf-dipping method were used for oral toxicity assays. The LC50 values (Lethal Concentration to kill 50% of insect population) of acetamiprid and Al- and PEG-nanoencapsulated formulations on third-instar larvae were 0.68, 0.04, and 0.08 ppm, respectively. Based on the highest relative potency, AL-encapsulated acetamiprid had the most toxicity. The content of energy reserve protein, glucose, and triglyceride and the activity of detoxifying enzymes esterase and glutathione S-transferase of the larvae treated by LC50 values of nanoformulations were also decreased. According to the current findings, the nanoencapsulation of acetamiprid by Al and PEG can increase its insecticidal performance in terms of lethal and sublethal toxicity.
Milk–Ta2O5 Hybrid Memristors with Crossbar Array Structure for Bio-Organic Neuromorphic Chip Applications
Authors: Min Jin-Gi , Park Hamin , Cho Won-Ju
Published: August 2022

In this study, a high-performance bio-organic memristor with a crossbar array structure using milk as a resistive switching layer (RSL) is proposed. To ensure compatibility with the complementary metal oxide semiconductor process of milk RSL, a high-k Ta2O5 layer was deposited as a capping layer; this layer enables high-density, integration-capable, photolithography processes. The fabricated crossbar array memristors contain milk–Ta2O5 hybrid membranes, and they exhibit bipolar resistance switching behavior and uniform resistance distribution across hundreds of repeated test cycles. In terms of the artificial synaptic behavior and synaptic weight changes, milk–Ta2O5 hybrid crossbar array memristors have a stable analog RESET process, and the memristors are highly responsive to presynaptic stimulation via paired-pulse facilitation excitatory post-synaptic current. Moreover, spike-timing-dependent plasticity and potentiation and depression behaviors, which closely emulate long-term plasticity and modulate synaptic weights, were evaluated. Finally, an artificial neural network was designed and trained to recognize the pattern of the Modified National Institute of Standards and Technology (MNIST) digits to evaluate the capability of the neuromorphic computing system. Consequently, a high recognition rate of over 88% was achieved. Thus, the milk–Ta2O5 hybrid crossbar array memristor is a promising electronic platform for in-memory computing systems.
Using Femtosecond Laser Pulses to Explore the Nonlinear Optical Properties of Au NP Colloids That Were Synthesized by Laser Ablation
Authors: Ashour Mohamed , G. Faris Hameed , Ahmed Hanan , Mamdouh Samar , Thambiratnam Kavintheran , Mohamed Tarek
Published: August 2022

In this study, we experimentally investigated the nonlinear optical properties of Au nanoparticles (Au NPs) that were prepared in pure distilled water using the laser ablation method. The Au NPs were prepared using a nanosecond Nd:YAG laser with an ablation time of 5 or 10 min at a constant laser energy of 100 mJ. The structure and the linear optical properties of the Au NPs were investigated using a transmission electron microscope (TEM) and UV-visible spectrophotometer analysis, respectively. The TEM measurements showed that the average size of the Au NPs varied from 20.3 to 14.1 nm, depending on the laser ablation time. The z-scan technique was used to investigate the nonlinear refractive index (n2) and nonlinear absorption coefficient (γ) of the Au NPs, which were irradiated at different excitation wavelengths that ranged from 740 to 820 nm and at different average powers that ranged from 0.8 to 1.6 W. The Au NP samples exhibited a reverse saturable absorption (RSA) behavior that increased when the excitation wavelength and/or incident laser power increased. In addition, the Au NPs acted as a self-defocusing material whenever the excitation wavelength or incident power were modified.
Electronic Structures and Photoelectric Properties in Cs3Sb2X9 (X = Cl, Br, or I) under High Pressure: A First Principles Study
Authors: Wu Yanwen , Xiang Guangbiao , Zhang Man , Wei Dongmei , Cheng Chen , Leng Jiancai , Ma Hong
Published: August 2022

Lead-free perovskites of Cs3Sb2X9 (X = Cl, Br, or I) have attracted wide attention owing to their low toxicity. High pressure is an effective and reversible method to tune bandgap without changing the chemical composition. Here, the structural and photoelectric properties of Cs3Sb2X9 under high pressure were theoretically studied by using the density functional theory. The results showed that the ideal bandgap for Cs3Sb2X9 can be achieved by applying high pressure. Moreover, it was found that the change of the bandgap is caused by the shrinkage of the Sb-X long bond in the [Sb2X9]3− polyhedra. Partial density of states indicated that Sb-5s and X-p orbitals contribute to the top of the valence band, while Sb-5p and X-p orbitals dominate the bottom of the conduction band. Moreover, the band structure and density of states showed significant metallicity at 38.75, 24.05 GPa for Cs3Sb2Br9 and Cs3Sb2I9, respectively. Moreover, the absorption spectra showed the absorption edge redshifted, and the absorption coefficient of the Cs3Sb2X9 increased under high pressure. According to our calculated results, the narrow bandgap and enhanced absorption ability under high pressure provide a new idea for the design of the photovoltaic and photoelectric devices.
Temperature-Dependent Self-Powered Solar-Blind Photodetector Based on Ag2O/β-Ga2O3 Heterojunction
Authors: Park Taejun , Park Sangbin , Park Joon Hui , Min Ji Young , Jung Yusup , Kyoung Sinsu , Kang Tai Young , Kim Kyunghwan , Rim You Seung , Hong Jeongsoo
Published: August 2022

In this study, a high-photoresponsivity self-powered deep ultraviolet (DUV) photodetector based on an Ag2O/β-Ga2O3 heterojunction was fabricated by depositing a p-type Ag2O thin film onto an n-type β-Ga2O3 layer. The device characteristics after post-annealing at temperatures ranging from 0 to 400 °C were investigated. Our DUV devices exhibited typical rectification characteristics. At a post-annealing temperature of 300 °C, the as-fabricated device had a low leakage current of 4.24 × 10−11 A, ideality factor of 2.08, and a barrier height of 1.12 eV. Moreover, a high photo-responsivity of 12.87 mA/W was obtained at a 100 μW/cm2 light intensity at a 254 nm wavelength at zero bias voltage, the detectivity was 2.70 × 1011 Jones, and the rise and fall time were 29.76, 46.73 ms, respectively. Based on these results, the Ag2O/β-Ga2O3 heterojunction photodetector operates without an externally applied voltage and has high responsivity, which will help in the performance improvement of ultraviolet sensing systems.
Evaluation of Dosimetric Effect of Bone Scatter on Nanoparticle-Enhanced Orthovoltage Radiotherapy: A Monte Carlo Phantom Study
Authors: Sadiq Afia , Chow James C. L.
Published: August 2022

In nanoparticle (NP)-enhanced orthovoltage radiotherapy, bone scatter affected dose enhancement at the skin lesion in areas such as the forehead, chest wall, and knee. Since each of these treatment sites have a bone, such as the frontal bone, rib, or patella, underneath the skin lesion and this bone is not considered in dose delivery calculations, uncertainty arises in the evaluation of dose enhancement with the addition of NPs in radiotherapy. To investigate the impact of neglecting the effect of bone scatter, Monte Carlo simulations based on heterogeneous phantoms were carried out to determine and compare the dose enhancement ratio (DER), when a bone was and was not present underneath the skin lesion. For skin lesions with added NPs, Monte Carlo simulations were used to calculate the DER values using different elemental NPs (gold, platinum, silver, iodine, as well as iron oxide), in varying NP concentrations (3–40 mg/mL), at two different photon beam energies (105 and 220 kVp). It was found that DER values at the skin lesion increased with the presence of bone when there was a higher atomic number of NPs, a higher NP concentration, and a lower photon beam energy. When comparing DER values with and without bone, using the same NP elements, NP concentration, and beam energy, differences were found in the range 0.04–3.55%, and a higher difference was found when the NP concentration increased. By considering the uncertainty in the DER calculation, the effect of bone scatter became significant to the dose enhancement (>2%) when the NP concentration was higher than 18 mg/mL. This resulted in an underestimation of dose enhancement at the skin lesion, when the bone underneath the tumour was neglected during orthovoltage radiotherapy.
Trends in the Development of Electronic Noses Based on Carbon Nanotubes Chemiresistors for Breathomics
Authors: Freddi Sonia , Sangaletti Luigi
Published: August 2022

The remarkable potential of breath analysis in medical care and diagnosis, and the consequent development of electronic noses, is currently attracting the interest of the research community. This is mainly due to the possibility of applying the technique for early diagnosis, screening campaigns, or tracking the effectiveness of treatment. Carbon nanotubes (CNTs) are known to be good candidates for gas sensing, and they have been recently considered for the development of electronic noses. The present work has the aim of reviewing the available literature on the development of CNTs-based electronic noses for breath analysis applications, detailing the functionalization procedure used to prepare the sensors, the breath sampling techniques, the statistical analysis methods, the diseases under investigation, and the population studied. The review is divided in two main sections: one focusing on the e-noses completely based on CNTs and one reporting on the e-noses that feature sensors based on CNTs, along with sensors based on other materials. Finally, a classification is presented among studies that report on the e-nose capability to discriminate biomarkers, simulated breath, and animal or human breath.
Cognitive Relapse in Multiple Sclerosis: New Findings and Directions for Future Research
Authors: Weinstock Zachary L. , Benedict Ralph H. B.
Published: August 2022

Multiple sclerosis (MS) is a chronic inflammatory, demyelinating disease of the central nervous system, often presenting with brain atrophy and cognitive impairment (CI). In the relapsing–remitting phenotype, cognitive performance is increasingly recognized to decline acutely during MS relapse, with varying degrees of recovery afterwards. Therefore, CI in MS may result from incomplete recovery from episodes of so-called “cognitive relapse”, gradual neurodegeneration, or both. Among a variety of validated measures of cognitive performance, the Symbol Digit Modalities Test (SDMT) represents the most sensitive measure of cognitive decline and is easily translated to clinical practice. In fact, cognitive relapse identified using the SDMT has been reported in clinically relapsing cohorts as well as in individuals with no other neurological signs, suggesting that routine cognitive assessment may be necessary to fully appreciate the extent of a patient’s disease activity. The aim of this narrative review is as follows: (1) to provide the historical context for neuropsychological assessment in MS, (2) to provide a summation of key studies describing the cognitive relapse phenomenon, and (3) to discuss current gaps in our knowledge and highlight avenues for future research.
WIC Participants’ Perceptions of the Cash-Value Benefit Increase during the COVID-19 Pandemic
Authors: Halverson McKenna , Karpyn Allison
Published: August 2022

Recent changes to the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) Cash-Value Benefit (CVB), which provides participants with money to spend on fruits and vegetables, have the potential to reduce disparities in healthy food access and food insecurity that were exacerbated by the COVID-19 pandemic. However, few studies have examined how the changes to the CVB allotment that occurred during the pandemic influenced WIC participants’ perceptions of the benefit or their fruit and vegetable purchasing and consumption. To address this gap, we conducted semi-structured interviews with 51 WIC participants in Wilmington, Delaware. Survey measures included demographic questions, the Hunger Vital Sign food insecurity screener, and open-ended questions regarding perceptions of the CVB increase and its influence on participants’ fruit and vegetable purchasing and consumption. Data were analyzed using a hybrid inductive and deductive coding approach. The results demonstrate that higher CVB allotments increased WIC participants’ purchasing and consumption of fruits and vegetables, increased the frequency of their shopping occasions, and enhanced their dietary variety. Our findings also suggest that WIC participants highly value the increased CVB. Consequently, maintaining the increased CVB allotment could improve the nutritional outcomes of low-income mothers, infants, and children participating in WIC.
In Vitro Digestibility of Minerals and B Group Vitamins from Different Brewers’ Spent Grains
Authors: Fărcaș Anca Corina , Socaci Sonia Ancuța , Chiș Maria Simona , Martínez-Monzó Javier , García-Segovia Purificación , Becze Anca , Török Anamaria Iulia , Cadar Oana , Coldea Teodora Emilia , Igual Marta
Published: August 2022

Brewers’ spent grain (BSG), the main by-product of the brewing industry, is a rich source of minerals and water-soluble vitamins such as thiamine, pyridoxine, niacin, and cobalamin. Bioaccessibility through in vitro digestion is an important step toward the complete absorption of minerals and B group vitamins in the gastrointestinal system. Inductively coupled plasma optical emission spectrometry (ICP-OES) together with inductively coupled plasma quadrupole mass spectrometry (ICP-MS) was used for the quantification of the macro- and micro-minerals. An ultra-high performance liquid chromatography (UHPLC) system coupled with a diode array detector (DAD) was used for B group vitamin identification. Four different industrial BSG samples were used in the present study, with different percentages of malted cereals such as barley, wheat, and degermed corn. Calcium’s bioaccessibility was higher in the BSG4 sample composed of 50% malted barley and 50% malted wheat (16.03%), while iron presented the highest bioaccessibility value in the BSG2 sample (30.03%) composed of 65% Pale Ale malt and 35% Vienna malt. On the other hand, vitamin B1 had the highest bioaccessibility value (72.45%) in the BSG3 sample, whilst B6 registered the lowest bioaccessibility value (16.47%) in the BSG2 sample. Therefore, measuring the bioaccessibilty of bioactive BSG compounds before their further use is crucial in assessing their bioavailability.
Effect of Peripheral Magnetic Stimulation for Dysphagia Rehabilitation: A Systematic Review
Authors: Hwang Na-Kyoung , Park Ji-Su , Choi Jong-Bae , Jung Young-Jin
Published: August 2022

Recently, a therapeutic method to stimulate the suprahyoid muscle using peripheral magnetic stimulation for dysphagia rehabilitation has been reported. However, clinical evidence, application protocol, and intervention method remain unclear. Therefore, a systematic review of the published literature is needed. The objective of this study was to systematically review clinical studies of peripheral magnetic stimulation applied for rehabilitation of dysphagia. Issues to be considered in future studies are also suggested. This systematic review performed a literature search of four databases (Medline, Embase, CINAHL, and Web of Science) to identify relevant studies published on the application of repetitive peripheral magnetic stimulation (rPMS) for swallowing-related muscles between 2010 and 2022. Seven studies were reviewed. Randomized controlled trials and one-group pre–post, case study designs were included. In the included studies, rPMS was applied to strengthen the submental suprahyoid muscles. The intervention regime varied. The rPMS was applied at a frequency of 30 Hz for 2 s. Rest time ranged from 8 s to 27–28 s. The number of intervention sessions ranged from 2–3 to 30. The intensity ranged from pain-inducing minimum intensity (90% of maximum stimulus output) to non-painful intensity (70–80% of maximum intensity). The rPMS on the suprahyoid muscles had positive effects on physiological changes in the swallowing function, such as displacement of the hyoid bone, muscle strength (cervical flexor, jaw-opening force), swallowing safety, swallowing performance, and swallowing-related quality of life. Participants also reported little pain and adverse reactions during rPMS. Although rPMS is a therapeutic option that can help improve the swallowing function as a non-invasive stimulation method in the rehabilitation of dysphagia, clinical evidence is needed for the development of clear stimulation protocols and guidelines.
Validation of the Visual/Verbal Analogue Scale of Food Ingesta (Ingesta-VVAS) in Oncology Patients Undergoing Chemotherapy
Authors: Wijnhoven Hanneke A. H. , van der Velden Loïs , Broek Carolina , Broekhuizen Marleen , Bruynzeel Patricia , van Breen Antoinette , van Oostendorp Nanda , de Heer Koen
Published: August 2022

This study aimed to: (1) externally validate the Visual/Verbal Analogue Scale of food ingesta (ingesta-VVAS) that previously showed good discrimination between oncology patients who ingest more or less energy than required; (2) explore the discriminative properties of other questions. Dietitians performed 322 interviews in 206 adult oncology patients undergoing chemotherapy in two Dutch hospitals, including a 24-h dietary recall, assessment of the ingesta-VVAS and 12 additional questions related to reduced food intake. The ingesta-VVAS score was linearly associated with energy intake as % of Total Energy Expenditure (TEE) (standardized beta = 0.39, p < 0.001), with no differences between groups based on use of oral nutritional supplements, body mass index, in/outpatient setting or sex. The accuracy of the ingesta-VVAS score to predict low energy intake (<75% of TEE) was poor (Area Under the Receiver Operating Characteristic curve (AUC) = 0.668, 95% CI 0.603–0.733). The optimal multivariate model included the ingesta-VVAS score and a question on ‘feeling sick’ (AUC = 0.680, 95% CI 0.615–0.746). In conclusion, in our study the ingesta-VVAS discriminates poorly between oncology patients undergoing chemotherapy who ingest more or less energy than required. Adding a question on feeling sick only slightly improved model performance. Further external validation is warranted.
Association of Pyridoxal 5′-Phosphate with Sleep-Related Problems in a General Population
Authors: Ge Lin , Luo Jia , Zhang Liming , Kang Xiao , Zhang Dongfeng
Published: August 2022

The evidence on the relationship of pyridoxal 5′-phosphate (PLP) with sleep-related problems is limited and controversial. Notably, there is a lack of studies on the general population and studies of the dose–response relationship. Therefore, we conducted a cross-sectional study to examine the associations between serum PLP concentration and sleep-related problems (sleep quality and sleep duration) in adults, using the data of the National Health and Nutrition Examination Survey 2005–2010. High-performance liquid chromatography (HPLC) was used to test PLP in blood samples. Sleep quality and sleep duration were based on self-reported data, with sleep quality categorized as sleep disorder, trouble falling asleep, waking up during the night, and daytime sleepiness. The primary analyses utilized logistic regression models and restricted cubic spline. Compared with the first quartile (Q1), the odds ratios (ORs) and 95% confidence intervals (CIs) of daytime sleepiness for the Q2 and Q3 of serum PLP concentrations were 0.76 (0.59–0.99) and 0.78 (0.62–0.98), respectively. The relationship was only significant for males. Furthermore, a non-linear dose–response relationship was observed between serum PLP concentration and the risk of daytime sleepiness. Compared with the normal sleep duration group, serum PLP concentrations were negatively associated with the risks of very short, short, and long sleep duration, with relative risk ratios (RRRs) of 0.58 (0.43–0.81) (Q4), 0.71 (0.61–0.83) (Q4) and 0.62 (0.34–0.94) (Q3), respectively. The average serum PLP concentrations were higher in people with normal sleep duration, suggesting a non-linear dose–response relationship. Our study indicated that serum PLP concentrations were negatively associated with daytime sleepiness, and this association may only exist in males. Moreover, it was also inversely related to abnormal sleep duration (very short, short, long) compared to normal sleep duration.
Quantitative Structure–Activity Relationship Analysis of Isosteviol-Related Compounds as Activated Coagulation Factor X (FXa) Inhibitors
Authors: Gackowski Marcin , Szewczyk-Golec Karolina , Mądra-Gackowska Katarzyna , Pluskota Robert , Koba Marcin
Published: August 2022

Stevioside, one of the natural sweeteners extracted from stevia leaves, and its derivatives are considered to have numerous beneficial pharmacological properties, including the inhibition of activated coagulation factor X (FXa). FXa-PAR signaling is a possible therapeutic target to enhance impaired metabolism and insulin resistance in obesity. Thus, the goal of the investigation was a QSAR analysis using multivariate adaptive regression splines (MARSplines) applied to a data set of 20 isosteviol derivatives bearing thiourea fragments with possible FXa inhibitory action. The best MARS submodel described a strong correlation between FXa inhibitory activity and molecular descriptors, such as: B01[C-Cl], E2m, L3v, Mor06i, RDF070i and HATS7s. Five out of six descriptors included in the model are geometrical descriptors quantifying three-dimensional aspects of molecular structure, which indicates that the molecular three-dimensional conformation is of high significance for the MARSplines modeling procedure and obviously for FXa inhibitory activity. High model performance was confirmed through an extensive validation protocol. The results of the study not only confirmed the enhancement in pharmacological activity by the presence of chlorine in a phenyl ring, but also, and primarily, may provide the basis for searching for new active isosteviol analogues, which may serve as drugs or health-beneficial food additives in patients suffering from obesity and comorbidities.
Gut Microbiota Changes by an SGLT2 Inhibitor, Luseogliflozin, Alters Metabolites Compared with Those in a Low Carbohydrate Diet in db/db Mice
Authors: Hata Shinnosuke , Okamura Takuro , Kobayashi Ayaka , Bamba Ryo , Miyoshi Tomoki , Nakajima Hanako , Kitagawa Nobuko , Hashimoto Yoshitaka , Majima Saori , Senmaru Takafumi , Okada Hiroshi , Ushigome Emi , Nakanishi Naoko , Takakuwa Hiroshi , Sasano Ryoichi , Hamaguchi Masahide , Fukui Michiaki
Published: August 2022

In recent years, sarcopenic obesity has been considered central pathological factors in diabetes. This study aimed to compare the effect of luseogliflozin, a sodium-glucose co-transporter-2 inhibitor (SGLT2i), on sarcopenic obesity in comparison to that of a low-carbohydrate diet (LCD). Twenty-week-old male db/db mice were fed a normal diet (Ctrl), LCD, and normal diet with 0.01% w/w luseogliflozin (SGLT2i) for eight weeks. Skeletal muscle mass and grip strength decreased in the LCD group mice compared to those in the control group, while they increased in the SGLT2i group mice. The amino acid content in the liver, skeletal muscle, and serum were lower in the LCD group than those in the Ctrl group but increased in the SGLT2i group mice. Short-chain fatty acids in rectal feces were lower in the LCD group mice than those in the Ctrl group, whereas they were higher in the SGLT2i group mice. The abundance of Gammaproteobacteria, Enterobacteriaceae, Escherichia, Enterobacterales, and Bacteroides caccae species increased in the LCD group compared to the other two groups, whereas the abundance of Syntrophothermus lipocalidus, Syntrophomonadaceae family, Parabacteroidesdistasonis distasonis, and the genus Anaerotignum increased in the SGLT2i group. Luseogliflozin could prevent sarcopenic obesity by improving amino acid metabolism.
One-Carbon Metabolism Biomarkers and Risks of Incident Neurocognitive Disorder among Cognitively Normal Older Adults
Authors: Przybycien-Gaweda Paulina Maria , Lee Tih Shih , Lim Wee Shiong , Chong Mei Sian , Yap Philip , Cheong Chin Yee , Rawtaer Iris , Liew Tau Ming , Gwee Xinyi , Gao Qi , Yap Keng Bee , Ng Tze Pin
Published: August 2022

There is a lack of evidence supporting an association between folate and vitamin B12 exposure with cognitive outcomes. We examined serum folate and vitamin B12 and plasma homocysteine in 690 cognitively-normal adults (aged ≥ 55) from the Singapore Longitudinal Aging Study (SLAS-2) followed-up over 4.5 years on incident neurocognitive disorder (NCD): mild cognitive impairment (MCI) and dementia. At follow-up, 5.7% (39) of participants developed NCD (34 MCI and 5 dementia). Comparing with those who remained cognitively-normal, participants progressed to NCD had significantly lower mean baseline vitamin B12 (420 [SD ± 221] vs. 510 [SD ± 290] pmol/L, p = 0.026), higher homocysteine (14.6 [SD ± 4.2] vs. 12.9 [SD ± 4.3], p = 0.018) and lower one-carbon index (Z-scores: −0.444 [SD ± 0.819] vs. −0.001 [SD ± 0.990], p = 0.006). Adjusted for confounders, significant associations with incident NCD were found for lower vitamin B12 (per-SD OR = 2.10, 95%CI = 1.26–3.52), higher homocysteine (per-SD OR = 1.96, 95%CI = 1.18–3.24) and lower one-carbon index (per-SD OR = 1.67, 95%CI = 1.06–2.64). Folate was not significantly associated with progression to NCD. Notably, low B12 in the presence of high folate was significantly associated with incident NCD (adjusted OR = 3.81, 95%CI = 1.04–13.9). Low B12, high homocysteine, low B12 in the presence of high folate, and a one-carbon index of hypo-methylation were independently associated with progression to NCD among cognitively normal.
Comparing the Effects of Concord Grape (Vitis labrusca L.) Puree, Juice, and Pomace on Intestinal Morphology, Functionality, and Bacterial Populations In Vivo (Gallus gallus)
Authors: Agarwal Nikita , Shukla Viral , Kolba Nikolai , Jackson Cydney , Cheng Jacquelyn , Padilla-Zakour Olga I. , Tako Elad
Published: August 2022

This is a preliminary study evaluating the effect of different fractions of Concord grapes (Vitis labrusca L.) on the brush border membrane (BBM) morphology, duodenal gene expression, and specific gut bacterial populations. For this study, we utilized a unique intraamniotic approach, wherein, the test substances are administered into the amnion of the Gallus gallus egg (on day 17). The embryo orally consumes the amniotic fluid along with the injected test substance before the hatch. We randomly divided ~50 fertilized eggs into 5 groups including 6% grape (juice, puree, and pomace) along with controls (no injection and diluent—H2O). The grape juice was prepared by crushing the grapes; the grape residues were used as pomace. The grape puree included the grape skin, endocarp, mesocarp, and juice but not the seeds. On day 21, the hatch day, the blood, pectoral muscle, liver, duodenum, and large intestine were harvested. Our results showed no significant differences in blood glucose, pectoral glycogen level, or body weight. However, significant (p < 0.05) differences in duodenal and liver gene expression were observed between the treatment groups. The grape puree treatment resulted in higher Clostridium numbers and lower Bifidobacterium numbers when compared to all other groups. In summary, the dietary consumption of grape polyphenols has the potential to beneficially modulate aspects of intestinal health provided their concentration is limited.
Chemosensory Functions in Patients with Inflammatory Bowel Disease and Their Association with Clinical Disease Activity
Authors: Han Xingyu , Ordouie Ayda-Ayleen , Schmelz Renate , Hummel Thomas
Published: August 2022

Purpose: Decreased olfactory and gustatory functions are present in various systemic autoimmune diseases. However, little is known about the chemosensory functions of patients with inflammatory bowel disease (IBD). The present study aimed to investigate olfactory and gustatory functions in patients with IBD and their correlation with clinical disease activity. Methods: A total of 103 patients with IBD were included (52 men, 51 women, mean age 40.3 ± 1.2 years) in the present study. Chemosensory functions were assessed utilizing the “Sniffin’ Sticks” olfactory function test and “taste sprays” gustatory function test. The clinical disease activity of patients was graded as remission, mild, and moderate–severe. In addition, inflammatory markers (fecal calprotectin, C-reactive protein and blood leucocyte count) were recorded. Results: In total, 70% of IBD patients were normosmic, 30% were hyposmic, and none of them was functionally anosmic; 6% of the patients showed signs of hypogeusia. Patients with moderate–severe IBD reached a higher olfactory threshold score compared with patients with remission (p = 0.011) and mild IBD (p < 0.001). The BMI of IBD patients was inversely correlated with their olfactory threshold (r = −0.25, p = 0.010). Olfactory and gustatory function in IBD patients did not correlate with duration of disease, blood leucocyte count, CRP level, or fecal calprotectin level. However, patients’ olfactory function significantly increased after 4 months of TNF-α inhibitor treatment (p = 0.038). Conclusions: IBD patients are more likely to present with hyposmia. Olfactory thresholds were mainly affected. They were significantly associated with clinical disease activity and BMI. As shown in a subgroup, treatment with TNF-α inhibitors appeared to improve olfactory function.
Validation of the Diet Quality Questionnaire in Chinese Children and Adolescents and Relationship with Pediatric Overweight and Obesity
Authors: Wang Huan , Herforth Anna W. , Xi Bo , Zou Zhiyong
Published: August 2022

The low-burden Diet Quality Questionnaire (DQQ) has been developed to rapidly assess diet quality globally. Poor diet is often correlated with body size, and certain dietary risk factors can result in overweight and obesity. We aimed to examine the extent to which the DQQ captured food group consumption among children and adolescents in China, and to understand the association of several new indicators of diet quality scores derived from the DQQ with overweight and obesity, using the 2011 wave of the China Health and Nutrition Survey. The DQQ questions are constructed using sentinel foods—that is, food items that are intended to capture a large proportion of the population consuming the food groups. The overall Global Dietary Recommendations (GDR) score, GDR-Healthy score, and GDR-Limit score are novel indicators of diet quality that reflect dietary risk factors for non-communicable diseases derived from the DQQ questions. Multivariable logistic regression analysis was used to examine the associations of the GDR scores with overweight and obesity in the sample. The DQQ questions captured over 95% of children who consumed the food groups. Additionally, we found that the GDR-Limit score was positively associated with general obesity (odds ratio (OR) = 1.43, 95% confidence interval (CI): 1.17–1.74) and abdominal obesity (OR = 1.22, 95% CI: 1.05–1.43), whereas the overall GDR score was negatively related to general obesity (OR = 0.85, 95% CI: 0.74–0.97). The low-burden DQQ could be a valid tool to assess diet quality for the Chinese pediatric population aged 7–18 years. Poor diet quality, as determined by the GDR-Limit score, is associated with the increased risk of obesity in Chinese children and adolescents.
Exploring the Fibrin(ogen)olytic, Anticoagulant, and Antithrombotic Activities of Natural Cysteine Protease (Ficin) with the κ-Carrageenan-Induced Rat Tail Thrombosis Model
Authors: Yang Hye Ryeon , Hwang Du Hyeon , Prakash Ramachandran Loganathan Mohan , Kim Jong-Hyun , Hong Il-Hwa , Kim Suk , Kim Euikyung , Kang Changkeun
Published: August 2022

Although fibrinolytic enzymes and thrombolytic agents help in cardiovascular disease treatment, those currently available have several side effects. This warrants the search for safer alternatives. Several natural cysteine protease preparations are used in traditional medicine to improve platelet aggregation and thrombosis-related diseases. Hence, this study aimed to investigate the effect of ficin, a natural cysteine protease, on fibrin(ogen) and blood coagulation. The optimal pH (pH 7) and temperature (37 °C) for proteolytic activity were determined using the azocasein method. Fibrinogen action and fibrinolytic activity were measured both electrophoretically and by the fibrin plate assay. The effect of ficin on blood coagulation was studied by conventional coagulation tests: prothrombin time (PT), activated partial thromboplastin time (aPTT), blood clot lysis assay, and the κ-carrageenan thrombosis model. The Aα, Bβ, and γ bands of fibrinogen are readily cleaved by ficin, and we also observed a significant increase in PT and aPTT. Further, the mean length of the infarcted regions in the tails of Sprague–Dawley rats was shorter in rats administered 10 U/mL of ficin than in control rats. These findings suggest that natural cysteine protease, ficin contains novel fibrin and fibrinogenolytic enzymes and can be used for preventing and/or treating thrombosis-associated cardiovascular disorders.