bluuebunny/crossref_metadata_2025 huggingface dataset:
Licht, Getting Work - Philadelphia, 1840-1950
Authors: Mugleston William
Published: April 2024

In this monograph Walter Licht, labor historian at the University of Pennsylvania, explores the dynamics of seeking, getting, and losing employment in a major American city over a period of a century. Licht opens with a description of the nature of Philadelphia business and industry-product diversity; a variety of work sites, including factories, small manufacturers, artisan shops, sweatshops, and the putting out system; specialization in operations and products; and a preponderance of noncorporate small-to-medium-sized family-owned firms. He then traces the entrance of workers into the job market and the efficacy of various institutions in getting work-schools, unions, apprentice programs, employment bureaus, and help-wanted ads.
Wandycz, The Price Of Freedom
Authors: Gentry Robert
Published: April 2024

Here is a book for students and instructors alike who lack the necessary historical context to make some sense of the often bewildering contemporary events occurring in the erstwhile Soviet Union and its satellites since the fall of the Berlin Wall and the apparent collapse of communism. Piotr Wandycz, Bradford Curfee Professor of History at Yale University and author of numerous works on Poland and East Central Europe, offers a crisp, readable survey of the history of East Central Europe since the middle ages without resorting to historical clichés, oversimplification, or national stereotypes. Within the compass of not quite 300 pages of text, he narrates the political and diplomatic history of the area and at the same time devotes considerable attention to the cultural, social, and economic developments that make the historical events understandable.
Baird Ed., Thought And Action - Readings In Western Intellectual History
Authors: Jenkins Jan
Published: April 2024

The search for primary source materials for use in a team-taught general education course on the history of philosophy led Forrest E. Baird, professor of philosophy at Whitworth College, in Spokane, Washington, to compile the excerpts included in Human Thought and Action: Readings in Western Intellectual History. Though Baird's original intention was to focus upon the historical role of rationalism, or the origin of human knowledge and action, in the Western intellectual tradition, the criteria for selection were expanded to allow the inclusion of works from "those who have had a major impact on the development of Western thought."
National Archives, The Bill Of Rights - Evolution Of Personal Liberties Kit
Authors: Woestman Kelly
Published: April 2024

The Bill of Rights: Evolution of Personal Liberties kit is an excellent example of scholars and teachers combining their expertise to produce a superior product for classroom instruction. As the tenth unit in this series of primary source material assembled by the National Archives, this set contains a selection of ten exercises that a teacher can use when discussing the Bill of Rights. This collection of 46 documents allows students to take a hands-on approach to their study of history.
Martel, Ed., Modern Germany Reconsidered - 1870-1945
Authors: Stauff Jon
Published: April 2024

Modem Germany Reconsidered is a collection of introductory historiographical and synthetic essays written by top scholars especially for undergraduate students beginning their study of German history. Students receive introductions to the historical discussions and interpretations of major events and periods (Second Reich, World War I, Nazism, Holocaust) and topics (economic history, women's history) without the acrimony that often laces debates played out in the professional journals.
Wexler, Westward Expansion - An Eyewitness History, Riley, A Place To Grow - Women In The American West
Authors: McDonald Archie
Published: April 2024

Westward Expansion is a part of the Eyewitness History series of Facts on File Press intended as a chronicle of "significant historical events or periods." Volumes in the series present numerous excerpts from a variety of sources, including memoirs, diaries, letters, newspaper articles, official documents-you name it. This volume in the series joins other entries that deal with the French Revolution and Napoleon, the Civil War and Reconstruction, World War I, Women's Suffrage, America's Gilded Age, and Vietnam.
Return Of Martin Guerre, Teaching History In Images, History In Words
Authors: Voeltz Richard
Published: April 2024

The Return of Martin Guerre received rave reviews from historians and history teachers when it was first released in this country in 1983. The distinguished Princeton University historian Natalie Zemon Davis had acted as historical consultant to the producers of the film while at the same time researching and writing her book, The Return of Martin Guerre (Cambridge: Harvard University Press, 1983). Critics and historians especially praised the film for its sensitive renderings of daily life in sixteenth-century France, of the mentalité of the period, i.e., how people actually view their world. Therefore, if for no other reason, the film is a significant learning resource for students of history from secondary to upper-level college courses.1 By acquainting students with the concept of mentalités, in this instance common reference points and symbols of an historical era, the entire concept of periodization in history, with its inherent artificiality, becomes a subject of suspicion to inquiring minds. And no era in history needs further reconsideration in terms of periodization than the Renaissance and Reformation.
Using Databases In The Social Studies Classroom
Authors: Foyle Harvey , Yates Bill
Published: April 2024

The knowledge base in history and the social studies is increasing exponentially. For example, Bosnia-Herzegovina is usually thought of in the context of World War I. Yet with the dissolution of the Eastern European communist block and the disintegration of Yugoslavia, this region is headline news once again. New countries, new capitals, new leaders, and an ever growing array of data. This knowledge explosion necessitates that students learn to manipulate information and make sense out of that information.
Klapisch-Zuber, Ed., A History Of Women In The West
Authors: Theilmann John
Published: April 2024

Medieval sources often ignored women or viewed them through male eyes, creating problems for historians. This collection of integrated essays, first published in Italy in 1990, is a new entry into the growing number of works dealing with women in the medieval West. Unlike some collections of essays, Silences of the Middle Ages was prepared so that the chapters fit together as a coherent whole.
Hoyt, Hirohito - The Emperor And The Man
Authors: Rines Lawrence
Published: April 2024

Since the end of World War II and the subsequent American occupation of Japan, an Imperial conspiracy behind Japanese militarism and expansionism in East Asia prior to and during World War II has always played well in American academia, the press, and in the mind of the general public. The publication of David Bergamini's popular 1300-page tome, Japan's Imperial Conspiracy (1971), served to reinforce this theory. Bergamini's analysis of Hirohito essentially agrees with the findings of the International Military Tribunal for the Far East that sat between 1946-1948, which convicted and sentenced some twenty-five well-known Japanese officials for war crimes, the best known being the infamous General Hideki Tojo.
Moses, Alexander Crummell - A Study Of Civilization And Discontent; Moses, Ed., Destiny And Race - Selected Writings, 1840-1898 - Alexander Crummell; Meier, A White Scholar And A Black Community, 1945-1965 - Essays And Reflections
Authors: Armstrong Thomas
Published: April 2024

In the tradition of biography, few would question the persistence of a Eurocentric and male bias. With the desirable goal of resurrecting otherwise unrepresented lives, biographers have increasingly presented us with individuals who might have been better known were it not for their gender or race. From this we have learned of the lives of many individuals, T. Thomas Fortune and Robert Vann, to name but two, who have been the subject of biography in recent years. A biography of Jackie Robinson gave us a much richer glimpse of mid-twentieth-century black and white lives. Our understanding of the black community and of America is the better for these efforts.
COMMUNICATION FOR DIGITAL LEADERSHIP: AN EXPLORATION TO THE FACTORS THAT CONTRIBUTE TO LOW INDEX
Authors: ABDUL MUSID NURHAFIZAH , MOHD MATORE MOHD EFFENDI@EWAN , A. HAMID AIDA HANIM
Published: April 2024

Digital leadership is important today because leadership development is a key element of sustainability education. To achieve the Sustainable Development Goals (SDGs), leaders should deploy resources, be visionary and ethical, and focus on long-term goals without neglecting values and principles. There are nine elements to assess the digital leadership of teachers. One of the crucial elements in digital leadership of teachers is communication. However, previous studies show that communication has a low index in digital leadership compared to the other elements. Therefore, this study aims to explore the factors that contribute to a low index of communication in digital leadership. In this paper, a qualitative approach using a semi-structured interview is adopted. The interview protocol consists of basic information about the participants, guidelines for the participants, interview questions, and appreciation for the participants. The interviews were conducted thoroughly, although there was only one interview question. The interviews were conducted online via the Zoom application. The interview participants were selected by using purposive sampling that involved four teachers from primary and secondary schools in Perak, Selangor and Sabah. The data was analysed using thematic analysis. The analysis revealed five factors: inefficient use of communication channels, inappropriate attitude of parents, lack of face-to-face communication, inappropriate teaching methods of teachers and limited internet access. The implications of this study point to the possibility of improving the use of communication channels in an efficient way, improving teaching methods so that students can understand what is being taught by teachers, and improving Internet access to increase the frequency of communication and diversify the methods of online communication between teachers and students. Stakeholders, administration and teachers should be more proactive in seeking solutions to overcome these challenges. It is suggested that a further study on communication in digital leadership be conducted, involving teachers from all states and using a quantitative approach and interviews among teachers in all types of Malaysian schools.
KUḺANTAI TATTEṬUPPUT TOṬARPĀṈA CAṬṬA NIYĀYAVIYAL ORU VARALĀṞṞUP PAKUPPĀYVU [THE JURISPRUDENCE OF CHILD ADOPTATION: A HISTORICAL REVIEW]
Authors: A.L. FATHIMA ZAMEETHA  , M.I.M. JAZEEL
Published: April 2024

Child adoption has been a longstanding practice in several legal traditions throughout human history. The national progression of child adoption has influenced the corresponding legislative modifications. Examining the Legal Framework of Child Adoption within the Context of Jurisprudence This article provides a historical analysis of the progression toward different legal frameworks. The qualitative examination of pertinent literature is predicated on investigating its content. Both individual countries and international organizations are actively engaged in developing legal frameworks that infringe upon children's rights and compromise their best interests. The adoption laws rooted in the penal code within the common law system have limited efficacy in mitigating instances of child abuse. In addition, international declarations, treaties, legal actions, and established norms protect the rights and interests of adopted children. Utilizing these tools is particularly noteworthy within the context of the intercountry adoption procedure. The legal laws pertaining to adoption have undergone significant development in various aspects, including the criteria for adopter eligibility, the requirement of adopter permission, the determination of adoptive family rights, and the establishment of support obligations.
Penerapan Teknik Self Management Untuk Menurunkan Kejenuhan Belajar Siswa Kelas VII
Authors: Faradisa Nidia Fullah
Published: April 2024

Students with learning boredom feel that the knowledge and skills obtained through learning are not progressing. KSaturation can also occur because students' learning processes have reached the limits of their physical abilities due to boredom and fatigue. One of the efforts through individual counseling with self-management techniques is expected to be able to reduce learning boredom for class VII students in Gresik. The technique for implementing this activity uses individual counseling with self-management techniques for related parties. Observations were carried out by digging up information and observing students' conditions in class by distributing questionnaires for the pre-test. From the results of interviews and observations with guidance and counseling teachers and homeroom teachers, there were several categories of students selected. Each class is represented by 6 students who experience learning boredom. There are 36 people from 3 classes. The decrease in learning boredom of selected class VII students can be observed through student learning outcomes each semester.
Peningkatan Kinerja Layanan dan Pengolahan Data Perangkat Desa Ulak Kembahang Melalui Pelatihan Aplikasi Komputer Ekonomi dan Bisnis
Authors: Imelda  , Yuniarti Emylia , Febrina Shelly , Novi Yarsah Waldi , Teguh Muhammad
Published: April 2024

The model of community service activities (PKM) is assistance in the form of technical guidance with direct practice in the Economics and Business Application Laboratory through the MS. Word and MS. Excel. PKM participants were 25 people, namely village officials including representatives of the Village Government, Village Representative Council (BPD), Community Empowerment Agency (LPM), PKK and Posyandu administrators and Youth Organization, Service activities in collaboration with the Economics and Business Application Laboratory. The results of the activity evaluation show that the average skills of 74.5 percent of participants are skilled and 20.5 percent are quite skilled for 8 statement items about skills using MS. Word, MS Excell, MS Power Point, creating Gmail and saving files on G-Drive.
Praktik Pemotongan Berat Timbangan pada Akad Jual Beli Cengkeh Kering: Perspektif Bisnis Islam
Authors: Umagapi Aidia , Hamizar Arizal , Karnudu Fitria
Published: April 2024

Penelitian ini bertujuan melihat lebih dalam mengenai fenomena jual beli cengkeh kering di Desa Fagudu. Sebagian dari pembeli cengkeh memotong berat timbangan yang sudah ditimbang yang telah menjadi kebiasaan di pasaran. Jenis penelitian yang digunakan dalam penelitian ini adalah jenis penelitian lapangan dengan pendekatan kualitatif. Sebagai single case study, penelitian ini dilaksanakan di Desa Fagudu Kabupaten Kepulauan Sula Provinsi Maluku Utara dengan metode pengumpulan data berupa wawancara, observasi maupun dokumentasi. Data yang diperoleh dianalisis dengan mereduksi data, display data hingga penarikan kesimpulan. Berdasarkan hasil penelitian diketahui bahwa praktik pemotongan berat timbangan pada akad jual beli cengkeh kering di Desa Fagudu dilakukan setelah adanya kesepakatan dari pihak pedagang dan penjual dan dilakukan atas dasar suka sama suka. Dari sudut pandang Ekonomi Islam, praktik pemotongan berat timbangan yang sudah menjadi kebiasaan masyarakat Desa Fagudu ini tidak bertentangan dengan ajaran Islam, karena potongan dilakukan setelah adanya persetujuan atau kerelaan dari kedua belah pihak sehingga tidak ada unsur paksaan di dalamnya.
The Role of the Halal Label in Purchasing Bottled Drinking Water: A Sharia Business Management Perspective
Authors: Hatapayo Siti Nur F. , Karnudu Fitria , Hamizar Arizal
Published: April 2024

The research aims to determine the role of the halal label in purchasing decisions for Ayudes Packaged Drinking Water (AMDK) products in Ambon City and what the role of the halal label is in reviewing sharia business management. This research was carried out in the Ambon City community and at the Ayudes AMDK company using qualitative methods. The data collection techniques used were observation, interviews, and documentation with a number of informants who were directly involved in the research. The results of this research show that the decision to purchase Ayudes bottled drinking water in Ambon City based on the role of the halal label shows that consumers do not pay attention to the inclusion of a halal label on the product packaging and pay more attention to the quality and location of the bottled water factory.
A relação entre o esgotamento emocional com os possíveis transtornos psiquiátricos: Ansiedade, depressão e burnout em estudantes de Medicina
Authors: Araújo João Pedro Gama , Silva Rafael Correia de Sousa da
Published: April 2024

Ao ingressar na faculdade de Medicina, observa-se um declínio significativo na saúde mental, e piora com o avançar da graduação. Diversos fatores são descritos como potencialmente estressantes em estudantes de Medicina, como o ambiente acadêmico altamente estressante, competitividade, privação de sono, carga de trabalho excessiva, pressão de colegas, fatores curriculares, afetivos e institucionais. O presente estudo, trata-se de uma revisão bibliográfica que tem como objetivo analisar o espectro das manifestações psiquiátricas em estudantes de Medicina e a relação com a exaustão mental destes indivíduos. O esgotamento não é dependente, puramente, de fatores predisponentes individuais, como expressão emocional ou meios de enfrentamento de estresse, mas também, está interligado a fatores externos, como o molde do sistema educacional e pode gerar impactos negativos na vida acadêmica e pessoal dos alunos. Entre os alunos do ciclo clínico, representados como sendo do 3°, 4° e 5° anos, a prevalência de burnout foi maior entre os alunos do terceiro e quarto anos, e esta maior prevalência pode ser devida à transição das ciências básicas para as ciências clínicas. É necessário a intervenção institucional, do corpo docente e de programas de apoio educacional, para o reconhecimento precoce, abordagem prática e direcionada como mecanismos de contenção de repercussões clínicas e prejuízos pessoais aos estudantes de Medicina.
Evidence and Transnational Punitive Enforcement Proceedings in the European Union
Authors:   , de Vries Aart Jacob
Published: April 2024

In many fields, enforcement of EU policies is performed by the Member States. In principle, Member States are free to decide how and by which means they enforce such policies, albeit under the observance of EU legal principles and harmonised rules. While differences between Member States are inevitable, especially at the interface of criminal and administrative enforcement, they should not create legal obstacles to effective and rule of law-based enforcement in cross-border cases.

In his dissertation, Aart de Vries analyses the legal principles and rules that determine the gathering, sharing, and use of evidence by EU Member States’ administrative and judicial authorities in cross-border enforcement cases to answer an important and current question: do these legal principles and rules enable effective and rule of law-based transnational punitive enforcement of EU policies?

De Vries looks into supranational EU law, and national law in The Netherlands, Germany, and France to provide a detailed analysis of the applicable legal framework. On the basis of his findings, De Vries presents a convincing answer to the aforementioned question, and puts forward various recommendations to enhance transnational punitive enforcement in the EU. In this light, the dissertation provides insights for academics and practitioners with an interest in punitive (criminal and administrative) enforcement of EU law.
Rebalancing treatment outcomes in rectal cancer
Authors:   , Verweij Maria Elisabet
Published: April 2024

Risks and benefits of short course radiotherapy with prolonged interval to
surgery
In order to counsel intermediate risk rectal cancer patients on the choice
between short course radiotherapy with immediate surgery versus short course
radiotherapy with prolonged interval to surgery, information on risks and benefits
of both treatment strategies is needed. Chapter 2 provides detailed information
on the toxicity during short course radiotherapy with prolonged interval to surgery.
Chapter 3 aims to confirm the advantage in the postoperative complication and
pathological complete response rate following SCRT with a prolonged interval
compared to direct surgery, that was demonstrated by the Stockholm III trial, in a
Dutch nationwide database.
Radiotherapy dose-escalation for improving organ preservation rates
The majority of patients would prefer organ preservation over surgical management,
but only a small proportion of patients reaches a cCR after standard neoadjuvant
treatments. The randomised RECTAL-BOOST trial aimed to increase the proportion
of pCR by administering dose-escalated CRT for locally advanced rectal cancer. In
chapter 4 the 2-year follow-up data on quality of life and oncological outcomes
of participants to the RECTAL-BOOST trial are analysed. The RECTAL-BOOST trial
followed the trials within cohorts (TwiCs) design. The experience of control patients
with this trial design is evaluated in chapter 5. Chapter 6 describes the protocol
of the preRADAR trial, wherein SCRT will be dose-escalated for intermediate risk
rectal cancer using the new technique of magnetic resonance-guided radiotherapy.
Combine and conquer as a strategy to treat RAS-driven cancers
Authors:   , Jansen Robin Alexandra
Published: April 2024

Cancer is a collective term for many different diseases. There are more than a 100 different types of cancer, each of which can be considered a different disease that requires its own treatment due to its unique characteristics. What all cancers have in common, however, is that cancer is primarily a genetic disease that can be caused by changes in the DNA, also known as mutations, that eventually lead to the production of mutated proteins and the dysregulation of cellular processes such as cell division, growth, migration, and survival. Such mutations in the DNA can occur 'spontaneously' as well as due to external factors such as smoking and UV radiation. Cancer cells that contain such mutations in specific genes have generally become dependent on these mutations for their disrupted cell division and survival.

The discovery that cancer cells have generally become dependent on these mutations has led to the development of a whole new class of drugs and a breakthrough in cancer treatment. 'Precision medicine' or 'targeted therapy' uses targeted cancer drugs ('magic bullets') that specifically block the mutated proteins on which the cancer cell depends. As a result of this personalized approach to cancer, cancer patients can receive individual treatment based on the unique mutation profile of their tumor. This increases the chance of success of the treatment and reduces the risk of side effects. In addition, targeted therapy is generally less harmful and results in superior progression-free survival compared to conventional chemotherapy.

One of the first examples, and also the most successful example of this concept, is the specific blocking of the fused BCR-ABL protein by the drug imatinib in patients with chronic myeloid leukemia. This targeted approach has resulted in a massive increase in 10-year survival rate. Before the targeted cancer drug imatinib was used, less than 20% of patients were still alive 10 years after diagnosis. This survival rate has increased to about 83% with the imatinib treatment. As a result, the use of targeted cancer drugs seemed like a 'miracle cure' for cancer. Unfortunately, this approach was not as successful for most other cancers, and the initial enthusiasm and expectations of the use and development of targeted cancer drugs had to be adjusted. Cancer patients generally experience short-term relief due to resistance to the targeted cancer drugs which inevitably develops. By understanding how cancer cells are and/or become resistant to targeted cancer drugs, it is possible to significantly improve cancer treatment. Cancer cells often use so-called resistance pathways to survive despite inhibition of the mutant protein. By also blocking this resistance pathway with a second cancer drug in combination with the first targeted drug, the cancer cell can no longer survive. The focus of this thesis is to 'combine and conquer' as a strategy for the treatment of cancer and to study why cancer cells are resistant to certain targeted therapies.
CORRIGENDUM FOR: Healthy Microbiome - a Mere Idea or a Sound Concept?
Authors: Najmanová L , Vídeňská P , Cahová M
Published: April 2024

On the basis of author’s request the publisher of Physiological Research decided to change the license of the article to CC BY license.
Changing ideologies about Arabic in Turkey and the consequences in Antioch
Authors: Istanbullu Suat
Published: April 2024

Arabic was unwanted by the designers and founders of the Republic of Turkey (founded in 1923) and prohibited in public space in the 1980s. Its status started changing in the late 1990s and 2000s, when it became increasingly offered in state education as a foreign language. By looking at the place of Arabic and the impact of the language policies of the Republic of Turkey then and now, the aim of this article is to investigate the consequences of these recent changes on the language ideologies of Turkish citizens in southern Turkey whose inherited familial language is Arabic but who are experiencing language shift to Turkish, the national language. Rooted in the sociolinguistics of multilingualism, this article, based on ethnographic fieldwork and interviews conducted in Antioch, shows that due to their religious and language background these citizens have lengthy experience of minoritization. Qualitative analysis of their language ideologies shows on the one hand a desire to maintain Arabic, but on the other hand conflicting views about the variety of Arabic they speak and a tendency to be influenced by pro-Turkish language ideologies. The paper concludes by re-evaluating the language shift they have experienced.
GAİBİN ÖLÜMÜ HAKKINDA KUVVETLİ OLASILIK BULUNMASININ GAİPLİK KARARININ VERİLEBİLMESİ İÇİN ARANMASI GEREKEN ÖZEL BİR KOŞUL OLUP OLMADIĞI KONUSUNDA BİR DEĞERLENDİRME
Authors: Bayram Aziz Erman
Published: April 2024

Bu çalışmanın amacı gaiplik durumlarını düzenleyen Türk Medenî Kanunu’nun 32. maddesinde yer alan “ölümü hakkında kuvvetli olasılık varsa” ifadesinin anlamının belirlenmesi ve gaiplik kararı verilebilmesi için kişinin ölümü hakkında kuvvetli olasılık bulunmasının, aranması gereken özel bir koşul olup olmadığının değerlendirilmesidir. Türk ve İsviçre hukuk öğretisinde konuya ilişkin başlıca eserlerin bazılarında kişinin ölümü hakkında kuvvetli olasılık bulunması, gaiplik kararının verilebilmesinin koşulları arasında özel olarak sayılmakta bazılarında ise sayılmamaktadır. Yargıtay uygulamasında ise kişinin ölümü hakkında kuvvetli olasılık bulunması, gaiplik kararı verilebilmesi için aranması gereken özel bir koşul olarak kabul edilmektedir. Bu çalışmada ölüm tehlikesi içinde kaybolma ve kendisinden uzun zamandan beri haber alınamama durumlarının tek başına, kişinin ölümü hakkında kuvvetli olasılık doğurabilen durumlar olduğu savunulmaktadır. Bu bağlamda ölüm tehlikesi içinde kaybolma ve kişiden uzun süredir haber alınamama ile kişinin ölümü hakkında kuvvetli olasılık bulunması arasında bir neden sonuç ilişkisi olduğu ileri sürülmektedir. Ayrıca Kanun’un 32. maddesinde yer alan “ölümü hakkında kuvvetli olasılık varsa” ifadesinin ölüm tehlikesi içinde kaybolma durumu bakımından gaiplik kararıyla ölüm karinesinin ayırt edilmesinde kullanılan; uzun süredir haber alınamama durumu bakımından ise haber alınamamanın makul bir gerekçeye dayanması hâlinde aradaki neden sonuç ilişkisinin kesilmesi sebebiyle gaiplik kararı verilmesini engelleyen bir ölçüt olarak değerlendirilmesi gerektiği sonucuna varılmaktadır.
A Digital Instructional Book: A Tool For Improving Students' Learning Outcomes on the Redox Reaction
Authors:   Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Pendidikan Ganesha, Indonesia, Redhana I Wayan , Sudria Ida Bagus Nyoman ,   Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Pendidikan Ganesha, Indonesia, Suardana I Nyoman ,   Department of Physics and Science Learning, Faculty of Mathematics and Natural Sciences, Universitas Pendidikan Ganesha, Indonesia
Published: April 2024

The rapid development of information and communication technology demands the production of digital products. This study aims to produce a digital instructional book based on Balinese local cultures to enhance the learning outcomes of high school students. The development of this book used the ADDIE model. The analysis phase included an analysis of the chemistry curriculum, teachers’ and students’ opinions about digital books, digital learning, and Balinese local cultures related to the chemistry content. The design phase included creating a framework and preparing a digital instructional book based on Balinese local cultures. The development phase included validation by content, language, and media experts. The implementation phase included testing the practicality and effectiveness of the book. The evaluation phase was carried out at all phases of the ADDIE model. The characteristics of this book were that it was made in a digital form and integrated with local Balinese cultures. It met the criteria of being a very valid category based on content, language, and media aspects. It also met a practical and effective category to improve students’ learning outcomes. It is very useful to enhance chemistry learning outcomes in the era of the Industrial Revolution 4.0.
Perbandingan Teknik Isolasi DNA pada Daun dan Kayu Sonokeling (Dalbergia latifolia)
Authors: Susila  , I Putu Gede P. Damayanto  , Kusumadewi Sri Yulita  , Nawwall Arrofaha
Published: April 2024

Sonokeling (Dalbergia latifolia) is a hardwood plant, resistant to termite attack, its wood has beautiful patterned fibers and textures, so it is widely used for furniture in luxury categories with high economic value. According to CITES (Convention on International Trade in Endagered Species of Wild Fauna and Flora), rosewood trade status is included in appendix II. In the trade of rosewood in both domestic and international markets, there are relatively many obstacles and difficulties in identifying the origin of rosewood. It is very important to establish a reference on the identity of wood based on the location of origin as an effort to prevent illegal trade, namely by using a molecular-based identification approach (DNA fingerprinting). In the isolation of rosewood DNA, special techniques are required due to its hard wood characteristics. This study aims to compare DNA isolation techniques from rosewood and rosewood leaves using the CTAB (Cetyl Trimethyl Ammonium Bromide) protocol and the DNA Mini Kit Plant protocol. The research used molecular biology-based identification methods. PCR results showed that the DNA isolation protocol using the commercial kit outperformed the CTAB protocol for both leaf and wood samples. DNA isolation from wood samples was more difficult than leaf samples. Overall, the best protocol for DNA isolation from rosewood leaves and wood was to use a commercial kit.
New-onset egg allergy in an adult: A case report
Authors: Fujiwara Michimasa , Kimura Takashi , Ohira Junya , Inotani Motohiro , Sakane Tomoko , Iwase Mizue , Yamashita Sadanori , Araki Tooru
Published: April 2024

Most adult cases of hen's egg allergy are carried over from childhood, and new-onset adult cases are rare. Such cases may result from cross-reactivity or sensitization by inhalation. Here we present a rare case of adult-onset egg allergy due to monosensitization to ovalbumin (Gal d 2) with an unclear sensitization pathway. A 27-year-old woman developed recurrent gastrointestinal symptoms after ingestion of raw and under-cooked eggs. She had never suffered from atopic dermatitis or food allergies. She had never kept birds as pets and had no history of exposure to egg allergens. Prick to prick testing was positive only with raw egg white. Specific IgE testing revealed monosensitization to Gal d 2. She was advised to avoid raw and undercooked eggs and her symptoms resolved. In the management of adult-onset egg allergy, evaluation of allergen components will lead to appropriate elimination guidelines, and investigation of sensitization pathways may help identify the cause of this disease.
Analyzing cooperative game theory solutions: core and Shapley value in cartesian product of two sets
Authors: Slime Mekdad , El Kamli Mohammed , Ould Khal Abdellah
Published: April 2024

The core and the Shapley value stand out as the most renowned solutions for addressing sharing problems in cooperative game theory. These concepts are widely acknowledged for their effectiveness in tackling negotiation, resource allocation, and power dynamics. The present study aims to extend various notions of cooperative games from the standard set N to a new class of cooperative games defined on the cartesian product N×N′ (utilizing the specific coalition A*B). This extension encompasses fundamental concepts such as rationality, core, and Shapley value. The findings presented in this study demonstrate that the core concept as a solution yields a set of imputations without favoring any specific point within the set, in contrast to the Shapley value, which offers a singular solution. Moreover, the results confirm that the Shapley value satisfies the conditions defining the core of a game. Through both theoretical analysis and numerical findings, employing a practical example, it becomes evident that the Shapley value offers a more distinct solution to the sharing problem compared with the core solution.
Development and application of one-step multiplex Real-Time PCR for detection of three main pathogens associated with bovine neonatal diarrhea
Authors: Wang Chaonan , Wang Fang , Chang Jitao , Jiang Zhigang , Han Yuxin , Wang Meixi , Jing Bo , Zhao Aiyun , Yin Xin
Published: April 2024

Introduction

Neonatal calf diarrhea (NCD) is one of the most common diseases in calves, causing huge economic and productivity losses to the bovine industry worldwide. The main pathogens include bovine rotavirus (BRV), bovine coronavirus (BCoV), and Enterotoxigenic Escherichia coli (ETEC) K99. Since multiple infectious agents can be involved in calf diarrhea, detecting each causative agent by traditional methods is laborious and expensive.

Methods

In this study, we developed a one-step multiplex Real-Time PCR assay to simultaneously detect BRV, BCoV, and E. coli K99+. The assay performance on field samples was evaluated on 1100 rectal swabs of diseased cattle with diarrhea symptoms and compared with the conventional gel-based RT-PCR assay detect BRV, BCoV, and E. coli K99+.

Results

The established assay could specifically detect the target pathogens without cross-reactivity with other pathogens. A single real-time PCR can detect ~1 copy/µL for each pathogen, and multiplex real-time PCR has a detection limit of 10 copies/µL. Reproducibility as measured by standard deviation and coefficient of variation were desirable. The triple real-time PCR method established in this study was compared with gel-based PT-PCR. Both methods are reasonably consistent, while the real-time PCR assay was more sensitive and could rapidly distinguish these three pathogens in one tube. Analysis of surveillance data showed that BRV and BCoV are major enteric viral pathogens accounting for calves’ diarrhea in China.

Discussion

The established assay has excellent specificity and sensitivity and was suitable for clinical application. The robustness and high-throughput performance of the developed assay make it a powerful tool in diagnostic applications and calf diarrhea research. ​
Cardioprotective effects of high-altitude adaptation in cardiac surgical patients: a retrospective cohort study with propensity score matching
Authors: Lei Li , Liu Mengxue , Ma Die , Lei Xia , Zeng Si , Li Peng , Huang Keli , Lyu Juanjuan , Lei Qian
Published: April 2024

Background

The cardioprotective effect of remote ischemia preconditioning in clinical studies is inconsistent with experimental results. Adaptation to high-altitude hypoxia has been reported to be cardioprotective in animal experiments. However, the clinical significance of the cardioprotective effect of high-altitude adaptation has not been demonstrated.

Methods

A retrospective cohort study with propensity score matching was designed to compare the outcomes of cardiac surgery between highlanders and lowlanders in a tertiary teaching hospital. The data of adult cardiac surgical patients from January 2013 to December 2022, were collected for analysis. Patients with cardiopulmonary bypass and cardioplegia were divided into a low-altitude group (<1,500 m) and a high-altitude group (≥1,500 m) based on the altitude of their place of residence.

Results

Of 3,020 patients, the majority (87.5%) permanently lived in low-altitude regions [495 (435, 688) m], and there were 379 patients (12.5%) in the high-altitude group [2,552 (1,862, 3,478) m]. The 377 highlander patients were matched with lowlander patients at a ratio of 1:1. The high-altitude group exhibited a 44.5% reduction in the incidence of major adverse cardiovascular events (MACEs) compared with the low-altitude group (6.6% vs. 11.9%, P = 0.017). The patients in the moderate high-altitude subgroup (2,500–3,500 m) had the lowest incidence (5.6%) of MACEs among the subgroups. The level of creatinine kinase muscle-brain isoenzymes on the first postoperative morning was lower in the high-altitude group than in the low-altitude group (66.5 [47.9, 89.0] U/L vs. 69.5 [49.3, 96.8] U/L, P = 0.003).

Conclusions

High-altitude adaptation exhibits clinically significant cardioprotection in cardiac surgical patients.
Left atrial remodeling and voltage-guided ablation outcome in obese patients with persistent atrial fibrillation
Authors: Marzak Halim , Fitouchi Simon , Labani Aïssam , Hammann Justine , Ringele Romain , Kanso Mohamad , Cardi Thomas , Schatz Alexandre , Ohlmann Patrick , Morel Olivier , Jesel Laurence
Published: April 2024

Background

Obesity is a risk factor for atrial fibrillation (AF). Data regarding left atrial (LA) remodeling in obese patients are scarce. Whether obesity favors AF recurrence after catheter ablation (CA) is still controversial. We assessed the distribution of epicardial atrial fat on computed tomography (CT), LA bipolar voltage, low-voltage zone (LVZ) extent, and the outcome of voltage-guided ablation of persistent AF in obese and non-obese patients.

Methods

A total of 139 patients with persistent AF undergoing a first voltage-guided ablation were enrolled and divided into two groups: 74 were non-obese and 65 were obese. Epicardial adipose tissue (EAT) was assessed on a CT scanner. LA endocardial voltage maps were obtained using a 3D mapping system in sinus rhythm. LVZ was defined as a bipolar peak-to-peak voltage amplitude <0.5 mV.

Results

LA volume, voltage, and EAT amount were similar in the two groups. LVZ was less frequent in obese patients [12 (18.8%) vs. 26 (35.1%), p = 0.05], particularly on the anterior wall. The posterior and lateral EATs were correlated with posterior and lateral LVZ extent, respectively, in obese patients. After 36 months of follow-up, the AF-free survival rate was similar. Lateral EAT [odds ratio (OR) 1.21, 95% confidence interval (CI) 1–1.4, p = 0.04] and P-wave duration (OR 1.03, 95% CI 1–1.05, p = 0.03), but not body mass index (BMI), were predictors of AF recurrence after CA.

Conclusion

In obese patients, LVZ was less marked than in non-obese patients with similar LA volumes, voltage, and EAT amounts. In obese patients, posterior and lateral EATs were correlated with posterior and lateral LVZ extents. Obese patients had a similar and favorable 36-month outcome after AF ablation. BMI was not predictive of AF recurrence.
MicroRNA analysis of medium/large placenta extracellular vesicles in normal and preeclampsia pregnancies
Authors: Awoyemi Toluwalase , Jiang Shuhan , Rahbar Maryam , Logentherian Prasanna , Collett Gavin , Zhang Wei , Cribbs Adam , Cerdeira Sofia , Vatish Manu
Published: April 2024

Background

Preeclampsia (PE) is a hypertensive disorder of pregnancy, affecting 2%–8% of pregnancies worldwide, and is the leading cause of adverse maternal and fetal outcomes. The disease is characterized by oxidative and cellular stress and widespread endothelial dysfunction. While the precise mechanisms are not entirely understood, the pathogenesis of PE is closely linked to placental dysfunction and, to some extent, syncytiotrophoblast extracellular vesicle release (STB-EVs). These vesicles can be divided into the less well-studied medium/large EVs (220–1,000 nm) released in response to stress and small EVs (<220 nm) released as a component of intercellular communication. The previously described production of m/lSTB-EVs in response to cellular stress combined with the overwhelming occurrence of cellular and oxidative stress in PE prompted us to evaluate the microRNAome of PE m/lSTB-EVs. We hypothesized that the microRNAome profile of m/lSTB-EVs is different in PE compared to normal pregnancy (NP), which might permit the identification of potential circulating biomarkers not previously described in PE.

Methods/study design

We performed small RNA sequencing on medium/large STB-EVs isolated from PE and NP placentae using dual-lobe ex vivo perfusion. The sequencing data was bioinformatically analyzed to identify differentially regulated microRNAs. Identified microRNAs were validated with quantitative PCR analysis. We completed our analysis by performing an in-silico prediction of STB-EV mechanistic pathways.

Results

We identified significant differences between PE and NP in the STB-EVs micro ribonucleic acid (microRNA) profiles. We verified the differential expression of hsa-miR-193b-5p, hsa-miR-324-5p, hsa-miR-652-3p, hsa-miR-3196, hsa-miR-9-5p, hsa-miR-421, and hsa-miR-210-3p in the medium/large STB-EVs. We also confirmed the differential abundance of hsa-miR-9-5p in maternal serum extracellular vesicles (S EVs). In addition, we integrated the results of these microRNAs into the previously published messenger RNA (mRNA) data to better understand the relationship between these biomolecules.

Conclusions

We identified a differentially regulated micro-RNA, hsa-miR-9-5p, that may have biomarker potential and uncovered mechanistic pathways that may be important in the pathophysiology of PE.
Low rates of myocardial fibrosis and ventricular arrhythmias in recreational athletes after SARS-CoV-2 infection
Authors: Miljoen Hielko , Favere Kasper , Van De Heyning Caroline , Corteville Ben , Dausin Christophe , Herbots Lieven , Teulingkx Tom , Bekhuis Youri , Lyssens Malou , Bogaert Jan , Heidbuchel Hein , Claessen Guido
Published: April 2024

Introduction

High rates of cardiac involvement were reported in the beginning of the coronavirus disease 2019 (COVID-19) pandemic. This led to anxiety in the athletic population. The current study was set up to assess the prevalence of myocardial fibrosis and ventricular arrhythmias in recreational athletes with the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

Methods

Consecutive adult recreational athletes (≥18 years old, ≥4 h of mixed type or endurance sports/week) underwent systematic cardiac evaluation after a prior confirmed COVID-19 infection. Evaluation included clinical history, electrocardiogram (ECG), 5-day Holter monitoring, and cardiac magnetic resonance (CMR) imaging with simultaneous measurement of high-sensitive cardiac Troponin I. Data from asymptomatic or mildly symptomatic athletes (Group 1) were compared with those with moderate to severe symptoms (Groups 2–3). Furthermore, a comparison with a historical control group of athletes without COVID-19 (Master@Heart) was made.

Results

In total, 35 athletes (18 Group 1, 10 female, 36.9 ± 2.2 years, mean 143 ± 20 days following diagnosis) were evaluated. The baseline characteristics for the Group 1 and Groups 2–3 athletes were similar. None of the athletes showed overt myocarditis on CMR based on the updated Lake Louise criteria for diagnosis of myocarditis. The prevalence of non-ischemic late gadolinium enhancement [1 (6%) Group 1 vs. 2 (12%) Groups 2–3; p = 0.603] or ventricular arrhythmias [1 Group 1 athlete showed non-sustained ventricular tachycardia (vs. 0 in Groups 2–3: p = 1.000)] were not statistically different between the groups. When the male athletes were compared with the Master@Heart athletes, again no differences regarding these criteria were found.

Conclusion

In our series of recreational athletes with prior confirmed COVID-19, we found no evidence of ongoing myocarditis, and no more detection of fibrosis or ventricular arrhythmias than in a comparable athletic pre-COVID cohort. This points to a much lower cardiac involvement of COVID-19 in athletes than originally suggested.
Pima County COVID-19 vaccine solutions dashboard project: lessons learned
Authors: Damalas Tina , Penney Eamon , Cullen Theresa , Dibner-Dunlap Aaron , English Cecelia , Gomez Jacob , Sapp Amanda , Selig Sara , Sutermaster Staci
Published: April 2024

Recent improvements in the accessibility of mapping tools and an increased recognition of the importance of leveraging data to inform public health operations has led to enthusiasm among public health departments to rapidly evolve their ability to analyze and apply data to programs. As the COVID-19 pandemic made evident, many health department data systems have been neglected for decades and data literacy among staff low. Significant federal dollars have been allocated to local health departments to modernize health systems. This case study recounts the effort to equip the Pima County Health Department with a highly sophisticated “COVID-19 Vaccines Solutions Dashboard” in 2021–2022, quantifying community vulnerability in the midst of the COVID-19 pandemic and shares key successes and challenges in process and outcomes that can guide other such dashboard initiatives. The experience informed the development of Pima' County Health Department's Data & Informatics Team as well as efforts to cultivate a more robust data culture throughout the department. Many health departments around the United States are in a similar position, and these lessons learned are widely applicable.