bluuebunny/crossref_metadata_2025 huggingface dataset:
A New Wave of Industrialization of PHA Biopolyesters
Authors: Koller Martin , Mukherjee Anindya
Published: February 2022

The ever-increasing use of plastics, their fossil origin, and especially their persistence in nature have started a wave of new innovations in materials that are renewable, offer the functionalities of plastics, and are biodegradable. One such class of biopolymers, polyhydroxyalkanoates (PHAs), are biosynthesized by numerous microorganisms through the conversion of carbon-rich renewable resources. PHA homo- and heteropolyesters are intracellular products of secondary microbial metabolism. When isolated from microbial biomass, PHA biopolymers mimic the functionalities of many of the top-selling plastics of petrochemical origin, but biodegrade in soil, freshwater, and marine environments, and are both industrial- and home-compostable. Only a handful of PHA biopolymers have been studied in-depth, and five of these reliably match the desired material properties of established fossil plastics. Realizing the positive attributes of PHA biopolymers, several established chemical companies and numerous start-ups, brand owners, and converters have begun to produce and use PHA in a variety of industrial and consumer applications, in what can be described as the emergence of the “PHA industry”. While this positive industrial and commercial relevance of PHA can hardly be described as the first wave in its commercial development, it is nonetheless a very serious one with over 25 companies and start-ups and 30+ brand owners announcing partnerships in PHA production and use. The combined product portfolio of the producing companies is restricted to five types of PHA, namely poly(3-hydroxybutyrate), poly(4-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxybutyrate-co-4-hydroxybutyrate), and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), even though PHAs as a class of polymers offer the potential to generate almost limitless combinations of polymers beneficial to humankind. To date, by varying the co-monomer type and content in these PHA biopolymers, their properties emulate those of the seven top-selling fossil plastics, representing 230 million t of annual plastics production. Capacity expansions of 1.5 million t over the next 5 years have been announced. Policymakers worldwide have taken notice and are encouraging industry to adopt biodegradable and compostable material solutions. This wave of commercialization of PHAs in single-use and in durable applications holds the potential to make the decisive quantum leap in reducing plastic pollution, the depletion of fossil resources, and the emission of greenhouse gases and thus fighting climate change. This review presents setbacks and success stories of the past 40 years and the current commercialization wave of PHA biopolymers, their properties, and their fields of application.
Activation of Calcium-Activated Chloride Channels Suppresses Inherited Seizure Susceptibility in Genetically Epilepsy-Prone Rats
Authors: Thomas Miracle , Simms Mark , N’Gouemo Prosper
Published: February 2022

Inherited seizure susceptibility in genetically epilepsy-prone rats (GEPR-3s) is associated with increased voltage-gated calcium channel currents suggesting a massive calcium influx resulting in increased levels of intraneuronal calcium. Cytosolic calcium, in turn, activates many processes, including chloride channels, to restore normal membrane excitability and limit repetitive firing of the neurons. Here we used EACT and T16Ainh-A01, potent activator and inhibitor of calcium-activated channels transmembrane protein 16A (TMEM16A), respectively, to probe the role of these channels in the pathophysiology of acoustically evoked seizures in the GEPR-3s. We used adult male and female GEPR-3s. Acoustically evoked seizures consisted of wild running seizures (WRSs) that evolved into generalized tonic-clonic seizures (GTCSs) and eventually culminated into forelimb extension (partial tonic seizures). We found that acute EACT treatment at relatively higher tested doses significantly reduced the incidences of WRSs and GTCSs, and the seizure severity in male GEPR-3s. Furthermore, these antiseizure effects were associated with delayed seizure onset and reduced seizure duration. Interestingly, the inhibition of TMEM16A channels reversed EACT’s antiseizure effects on seizure latency and seizure duration. No notable antiseizure effects were observed in female GEPR-3s. Together, these findings suggest that activation of TMEM16A channels may represent a putative novel cellular mechanism for suppressing GTCSs.
Prevalence of Pruritus and Association with Anxiety and Depression in Patients with Nonalcoholic Fatty Liver Disease
Authors: Boehlig Albrecht , Gerhardt Florian , Petroff David , van Boemmel Florian , Berg Thomas , Blank Valentin , Karlas Thomas , Wiegand Johannes
Published: February 2022

Patient-reported outcomes are important in nonalcoholic fatty liver disease (NAFLD). Pruritus is of special interest for evolving therapies with farnesoid X receptor (FXR) agonists. The aim of this study was to investigate the prevalence of pruritus in a real-life NAFLD cohort and analyze associations with anxiety and depression. Pruritus was assessed using a visual analogue- (VAS) and 5-D itch-scale (5-D). Anxiety and depression were evaluated by Beck’s-Depression-Inventory (BDI) and the Hospital Anxiety and Depression Scale (HADS-A, HADS-D). An optimal logistic regression model was found with a stepwise procedure to investigate variables associated with pruritus. In total, 123 NAFLD patients were recruited. VAS and 5-D were highly correlated (Spearman’s correlation coefficient 0.89). Moderate/severe pruritus was reported in 19% (VAS) and 21% (5-D) of patients. Anxiety and depression were present in 12% and 4% (HADS-A and HADS-D, respectively) and 12% (BDI) of cases. There was a significant association between VAS and BDI (p = 0.019). The final multivariate model for 5-D included diabetes mellitus (OR 4.51; p = 0.01), BDI (OR 5.98; p = 0.024), and HADS-A (OR 7.75; p = 0.011). One-fifth of NAFLD patients reported moderate or severe pruritus. 5-D was significantly associated with diabetes mellitus, depression, and anxiety. These findings should be tested in larger populations and considered in candidates for treatment with FXR agonists.
Functional Phenotypes of Intraplaque Macrophages and Their Distinct Roles in Atherosclerosis Development and Atheroinflammation
Authors: Mushenkova Nataliya V. , Nikiforov Nikita G. , Melnichenko Alexandra A. , Kalmykov Vladislav , Shakhpazyan Nikolay K. , Orekhova Varvara A. , Orekhov Alexander N.
Published: February 2022

Macrophages are the key inflammatory cell type involved in all stages of atherosclerosis development and progression, as demonstrated by numerous studies. Correspondingly, macrophages are currently regarded as a promising therapeutic target for the development of new treatment approaches. The macrophage population is heterogeneous and dynamic, as these cells can switch between a number of distinct functional states with pro- and anti-atherogenic activity in response to various stimuli. An atherosclerotic plaque microenvironment defined by cytokine levels, cell-to-cell interactions, lipid accumulation, hypoxia, neoangiogenesis, and intraplaque haemorrhage may guide local macrophage polarization processes within the lesion. In this review, we discuss known functional phenotypes of intraplaque macrophages and their distinct contribution to ahteroinflammation.
Study of Damage Quantification of Concrete Drainage Pipes Based on Point Cloud Segmentation and Reconstruction
Authors: Pang Gaozhao , Wang Niannian , Fang Hongyuan , Liu Hai , Huang Fan
Published: February 2022

The urban drainage system is an important part of the urban water cycle. However, with the aging of drainage pipelines and other external reasons, damages such as cracks, corrosion, and deformation of underground pipelines can cause serious consequences such as urban waterlogging and road collapse. At present, the detection of underground drainage pipelines mostly focuses on the qualitative identification of pipeline damage, and it is impossible to quantitatively analyze pipeline damage. Therefore, a method to quantify the damage volume of concrete pipes that combines surface segmentation and reconstruction is proposed. An RGB-D sensor is used to collect the damage information of the drainage pipeline, and the collected depth frame is registered to generate the pipeline’s surface point cloud. Voxel sampling and Gaussian filtering are used to improve data processing efficiency and reduce noise, respectively, and the RANSAC algorithm is used to remove the pipeline’s surface information. The ball-pivoting algorithm is used to reconstruct the surface of the segmented damage data and pipe’s surface information, and finally to obtain the damage volume. In order to evaluate, we conducted our research on real-world materials. The measurement results show that the method proposed in this paper measures an average relative error of 7.17% for the external damage volume of concrete pipes and an average relative error of 5.22% for the internal damage measurements of concrete pipes.
An Improved Model for Design Fatigue Load of Highway Bridges Considering Damage Equivalence
Authors: Fu Huawei , Zhou Xuhong , Zhou Qishi , Xiang Ping , Zhou Zhibin , Fu Qiang
Published: February 2022

Reasonable fatigue load should be determined before bridge fatigue analysis. However, the design frequency calculation method of the standard vehicle does not always make sense when the standard vehicle load model that is provided by existing standards is adopted, as the weights (equivalent coefficients) variation of different vehicle types are not considered from the perspective of damage equivalence. The method through direct damage calculation is workable but the process is usually laborious and time-consuming. To solve this problem, the traffic data of 35 highway sites involving 15 provinces in China were collected and the fatigue load spectrum were derived. The equivalent coefficients of each vehicle type at all of the 35 locations were calculated directly and the relationship with the corresponding gross vehicle weight was obtained formulaically through statistical analysis. Therefore, the design frequency of the standard vehicle can be calculated by the product of the actual frequency for a certain type of vehicle and the corresponding equivalent coefficient. The effectiveness of the proposed method was verified from the perspective of damage equivalence compared with the existing-standard method, and its flexibility and applicability for complex traffic conditions such as China were also demonstrated. In addition, three grades for the design frequency were put forward as references in relevant project design.
Treatment of Classic Hairy Cell Leukemia: Targeting Minimal Residual Disease beyond Cladribine
Authors: Bohn Jan-Paul , Dietrich Sascha
Published: February 2022

Classic hairy cell leukemia (HCL) is a rare indolent B-cell lymphoproliferative disorder characterized by profound pancytopenia and frequent infectious complications due to progressive infiltration of the bone marrow and spleen. Lacking effective treatment options, affected patients were confronted with a dismal survival prognosis of less than 5 years when the disease was first described in 1958. Tremendous therapeutic advances were accomplished with the introduction of purine analogues such as cladribine in the 1990s, facilitating a near-normal life expectancy in most HCL patients. Nevertheless, nearly all patients eventually relapse and require successive retreatments, while drug-associated myelotoxicity may accumulate and secondary malignancies may evolve. Detection of minimal residual disease (MRD) in a substantial portion of treated patients has become a surrogate for this still limited treatment efficacy. In the last decade, novel biologic insights such as identification of the driver mutation BRAF V600E have initiated the development and clinical investigation of new, chemotherapy-free, targeted drugs in HCL treatment, with encouraging efficacy in early clinical trials aimed at boosting eradication of MRD while optimizing drug tolerability. This review summarizes current clinical trials investigating treatment strategies beyond purine analogues in HCL and discusses clinically relevant obstacles still to overcome.
MicroRNA-101-3p Suppresses Cancer Cell Growth by Inhibiting the USP47-Induced Deubiquitination of RPL11
Authors: Park Jinyoung , Cho Moonsoo , Cho Jinhong , Kim Eunice EunKyeong , Song Eun Joo
Published: February 2022

MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that regulate a countless number of genes in the cell, and the aberrant expression of miRNA can lead to cancer. Here, we demonstrate that miR-101-3p regulates the RPL11–MDM2–p53 pathway by targeting ubiquitin-specific peptidase 47 (USP47), consequently inhibiting cancer cell proliferation. We confirm that miR-101-3p directly binds to the 3′-UTR region of the USP47 gene and inhibits USP47 expression. In addition, the overexpression of miR-101-3p suppresses cell proliferation in a p53-dependent manner. MiR-101-3p promotes interaction between RPL11 and MDM2 by inducing the translocation of RPL11 from the nucleolus to the nucleoplasm, thus preventing the MDM2-mediated proteasomal degradation of p53. However, these phenomena are restored by the overexpression of USP47, but not by its catalytically inactive form. Indeed, miR-101-3p regulates RPL11 localization and its interaction with MDM2 by inhibiting the USP47-induced deubiquitination of RPL11. Finally, the expression of miR-101-3p is downregulated in lung cancer patients, and the patients with low miR-101-3p expression exhibit a lower survival rate, indicating that miR-101-3p is associated with tumorigenesis. Together, our findings suggest that miR-101-3p functions as a tumor suppressor by targeting USP47 and could be a potential therapeutic target for cancers.
Allergen Immunotherapy: New Insights into an Old Treatment
Authors: Pitsios Constantinos
Published: February 2022

The continuous advancement of biomedical sciences has offered an extreme boost in improving the diagnosis and therapy of allergic diseases [...]
Dimerization of the Glucocorticoid Receptor and Its Importance in (Patho)physiology: A Primer
Authors: Timmermans Steven , Vandewalle Jolien , Libert Claude
Published: February 2022

The glucocorticoid receptor (GR) is a very versatile protein that comes in several forms, interacts with many proteins and has multiple functions. Numerous therapies are based on GRs’ actions but the occurrence of side effects and reduced responses to glucocorticoids have motivated scientists to study GRs in great detail. The notion that GRs can perform functions as a monomeric protein, but also as a homodimer has raised questions about the underlying mechanisms, structural aspects of dimerization, influencing factors and biological functions. In this review paper, we are providing an overview of the current knowledge and insights about this important aspect of GR biology.
Phylogenetics and Biogeography of Lilium ledebourii from the Hyrcanian Forest
Authors: Shokrollahi Shekoofeh , Yousefzadeh Hamed , Parisod Christian , Heshmati Gholamali , Bina Hamid , Ali Shujait , Amirchakhmaghi Narjes , Song Yigang
Published: February 2022

Lilium ledebourii (Baker) Boiss is one of the most endangered lilies, restricted to only a few small and fragmented areas in the Hyrcanian forest. This study aimed at evaluating the taxonomy of this unique Iranian lily and reconstructing divergence time from other species of the genus Lilium to address the role of this region in its diversification. Phylogenetic trees based on nuclear ITS and chloroplastic matK strongly supported the monophyly of the genus Lilium and division into subclades hardly matching prior morphological classifications. Biogeographic analyses using S-DIVA revealed East Asia as the ancestral range from where Lilium presented a multidirectional expansion towards North America, West-Central Asia, North Asia, and Europe. Diverging from ancestral Lilium during the beginning of Eocene (50 Ma; 95% HDP: 68.8–36.8). Specific members of Lilium colonized Iran (Western Asia) separated from the Clade IV (West-Central Asia and Europe lineage), and then yielded the Iranian L.ledebourri. Accordingly, the north of Iran appears to have promoted both long-term persistence and migration of Lily species from Asia to the Europe.
Bytecode Similarity Detection of Smart Contract across Optimization Options and Compiler Versions Based on Triplet Network
Authors: Zhu Di , Yue Feng , Pang Jianmin , Zhou Xin , Han Wenjie , Liu Fudong
Published: February 2022

In recent years, the number of smart contracts running in the blockchain has increased rapidly, accompanied by many security problems, such as vulnerability propagation caused by code reuse or vicious transaction caused by malicious contract deployment, for example. Most smart contracts do not publish the source code, but only the bytecode. Based on the research of bytecode similarity of smart contract, smart contract upgrade, vulnerability search and malicious contract analysis can be carried out. The difficulty of bytecode similarity research is that different compilation versions and optimization options lead to the diversification of bytecode of the same source code. This paper presents a solution, including a series of methods to measure the similarity of smart contract bytecode. Starting from the opcode of smart contract, a method of pre-training the basic block sequence of smart contract is proposed, which can embed the basic block vector. Positive samples were obtained by basic block marking, and the negative sampling method is improved. After these works, we put the obtained positive samples, negative samples and basic blocks themselves into the triplet network composed of transformers. Our solution can obtain evaluation results with an accuracy of 97.8%, so that the basic block sequence of optimized and unoptimized options can be transformed into each other. At the same time, the instructions are normalized, and the order of compiled version instructions is normalized. Experiments show that our solution can effectively reduce the bytecode difference caused by optimization options and compiler version, and improve the accuracy by 1.4% compared with the existing work. We provide a data set covering 64 currently used Solidity compilers, including one million basic block pairs extracted from them.
Stand-Age Derived Competition Indices Influence Individual Tree Mortality Model Prediction for Naturally Occurring Even-Aged Shortleaf Pine Stands
Authors: Saud Pradip , Lynch Thomas B. , Guldin James M. , Shrestha Sudip
Published: February 2022

To understand the influence of competition indices and post-thinning effects in predicting individual tree mortality, we developed two models, one without the effect of thinning and another, with the effect of thinning for naturally occurring even-aged natural shortleaf pine (Pinus echinata Mill.) stands in the Ozark and Ouachita National Forests in Oklahoma and Arkansas, USA. Over the period of 25 years, six re-measurements of an individual tree from each plot were collected between 1985 and 2014. The logistic function was used to model the probability of mortality for which the binary response variable was, ‘0’ for living and ‘1’ for dead trees, using iteratively weighted regression and mixed-effects model. Stand-age derived competition indices such as, the ratio of stand basal area to stand age (SBAG), ratio of individual diameter to stand age (DAG), and the quadratic mean diameter (QMD), were found significant in predicting the probability of mortality. These variables were also found to be effective in the thinning effect model. However, excluding the thinning variable resulted in better performance with the chi-square test based on mortality within mid-diameter classes. Thus, the mortality model suggests that over time, individual tree mortality is influenced more greatly by competition modified by stand age rather than by a post-thinning effect in even-aged naturally occurring shortleaf pine trees.
Physiological Performance and Biomass Growth of Different Black Locust Origins Growing on a Post-Mining Reclamation Site in Eastern Germany
Authors: Lange Christian A. , Knoche Dirk , Hanschke Robin , Löffler Sonja , Schneck Volker
Published: February 2022

Black Locust/Robinia can play an important role in land reclamation due to its pronounced nitrogen fixation capability, fast initial growth and relative high drought tolerance. Hence, we set up a trial to test 12 Black Locust clones and three provenances growing on sandy overburden material within the open cast lignite mine Welzow-Süd (South Brandenburg) in March 2014. Since then, biomass growth of the Black Locust trees was examined and physiological performance was studied on several occasions using chlorophyll a fluorescence and Dualex® measuring technique. Plant physiological measurements revealed differences in photosynthetic vitality (PIABS), although the PIABS values followed a similar pattern and sequences across the plot. While the genotypes Fra3 and Roy show the highest photosynthetic vitality, the clones Rog and Rob display the lowest PIABS mean values. Chlorophyll and phenol content as well as the nutrition supply of the test trees vary depending on their origin and site conditions. The annual biomass growth rate corresponds to photosynthetic vitality and both depend on weather conditions during the growing season. After six years, the growing biomass amounts to 14.7 Mg d.m. ha−1 for clone Rob and 44.8 Mg d.m. ha−1 for clone Fra3, i.e., 2.5 to 7.5 Mg d.m. ha−1 year−1. Our data demonstrate a good correlation between biophysical parameters and biomass growth. We, thus, infer that physiological measuring methods can be combined to strengthen predictions regarding the physiological performance of Black Locust origins.
The Nature and Size Fractions of Particulate Matter Deposited on Leaves of Four Tree Species in Beijing, China
Authors: Wang Huixia , Xing Yan , Yang Jia , Xie Binze , Shi Hui , Wang Yanhui
Published: February 2022

Particulate matter (PM) in different size fractions (PM0.1–2.5, PM2.5–10 and PM>10) accumulation on four tree species (Populus tomentosa, Platanus acerifolia, Fraxinus chinensis, and Ginkgo biloba) at two sites with different pollution levels was examined in Beijing, China. Among the tested tree species, P. acerifolia was the most efficient species in capturing PM, followed by F. chinensis, G. biloba, and P. tomentosa. The heavily polluted site had higher PM accumulation on foliage and a higher percentage of PM0.1–2.5 and PM2.5–10. Encapsulation of PM within cuticles was observed on leaves of F. chinensis and G. biloba, which was further dominated by PM2.5. Leaf surface structure explains the considerable differences in PM accumulation among tree species. The amounts of accumulated PM (PM0.1–2.5, PM2.5–10, and PM>10) increased with the increase of stomatal aperture, stomatal width, leaf length, leaf width, and stomatal density, but decreases with contact angle. Considering PM accumulation ability, leaf area index, and tolerance to pollutants in urban areas, we suggest P. acerifolia should be used more frequently in urban areas, especially in “hotspots” in city centers (e.g., roads/streets with heavy traffic loads). However, G. biloba and P. tomentosa should be installed in less polluted areas.
Methane Contributions of Different Components of Kandelia candel–Soil System under Nitrogen Supplementation
Authors: Feng Chen , You Huiming , Tan Fanglin , Han Jianliang , Yu Xiaoxue , You Weibin , He Dongjin
Published: February 2022

Kandelia candel is the most widely distributed tree species on the southeast coast of China and is also the main afforestation tree species along the coastal wetland. In recent years, inorganic nitrogen pollution has become increasingly severe, and investigating the effects of nitrogen input on methane emissions in Kandelia candel–soil systems has become significant from a global change perspective. However, the effect of nitrogen input on methane emissions in coastal wetland systems is still uncertain. The field tidal environment is complex and varied, and thus it is difficult to accurately control the amount of nitrogen in the system. Therefore, in order to accurately assess the effects of different concentrations of foreign nitrogen input on methane emission fluxes in a Kandelia candel–soil system, we use indoor tidal simulation experimental devices and design two simulation systems with and without plant planting to explore the difference of methane emission flux in this system under five nitrogen input concentrations: N0 (0 g N·m−2·a−1), N1 (5 g N·m−2·a−1), N2 (10 g N·m−2·a−1), N3 (20 g N·m−2·a−1), and N4 (30 g N·m−2·a−1). The results showed that: (1) The introduction of Kandelia candel promoted methane emissions in coastal wetland ecosystem. Under each nitrogen application concentration, the mean CH4 emission flux in the planting group was 42.98%, 65.59%, 40.87%, 58.93% and 39.23% higher than that in the non-planting group, respectively. (2) Nitrogen input significantly promoted methane emissions in both planted and non-planted environments, and the promoting effect showed as follows: N4 > N3 > N2 > N1 > N0. (3) After the introduction of Kandelia candel, the contribution of Kandelia candel and soil microorganisms to methane emissions was different under different concentrations of nitrogen addition. The contribution rate of Kandelia candel to CH4 emission flux of Kandelia candel–soil system ranged from 10.74% to 60.25%, with an average contribution rate of 37.30%. The changed soil microbes contributed 39.75% to 89.26% to the CH4 emission flux in the Kandelia candel–soil system, with an average contribution rate of 62.60%. Under N3 nitrogen application concentration, the emission flux of plant was the largest, which was significantly higher than that of the soil microbial pathway; at other concentrations, the methane emissions from the soil microbial pathway were greater than that of the plant pathway, and the contribution rate to the plant–soil system reached 60.25%. The results of this study provide an important basis for improving the estimation accuracy of carbon emissions in coastal waters and formulating policies for the restoration and protection of coastal wetlands.
Low-Cost Resin 3-D Printing for Rapid Prototyping of Microdevices: Opportunities for Supporting Aquatic Germplasm Repositories
Authors: Zuchowicz Nikolas C. , Belgodere Jorge A. , Liu Yue , Semmes Ignatius , Monroe William Todd , Tiersch Terrence R.
Published: February 2022

Germplasm repositories can benefit sustainable aquaculture by supporting genetic improvement, assisted reproduction, and management of valuable genetic resources. Lack of reliable quality management tools has impeded repository development in the past several decades. Microfabricated open-hardware devices have emerged as a new approach to assist repository development by providing standardized quality assessment capabilities to enable routine quality control. However, prototyping of microfabricated devices (microdevices) traditionally relies on photolithography techniques that are costly, time intensive, and accessible only through specialized engineering laboratories. Although resin 3-D printing has been introduced into the microfabrication domain, existing publications focus on customized or high-cost (>thousands of USD) printers. The goal of this report was to identify and call attention to the emerging opportunities to support innovation in microfabrication by use of low-cost (<USD 350) resin 3-D printing for rapid prototyping. We demonstrate that low-cost mask-based stereolithography (MSLA) 3-D printers with straightforward modifications can provide fabrication quality that approaches traditional photolithography techniques. For example, reliable feature sizes of 20 µm with dimensional discrepancy of <4% for lateral dimensions and <5% for vertical dimensions were fabricated with a consumer-level MSLA printers. In addition, alterations made to pre-processing, post-processing, and printer configuration steps improved print quality as demonstrated in objects with sharper edges and smoother surfaces. The prototyping time and cost of resin 3-D printing (3 h with USD 0.5/prototype) were considerably lower than those of traditional photolithography (5 d with USD 80/prototype). With the rapid advance of consumer-grade printers, resin 3-D printing can revolutionize rapid prototyping approaches for microdevices in the near future, facilitating participation in interdisciplinary development of innovative hardware to support germplasm repository development for aquatic species.
Experimental Techniques against RANS Method in a Fully Developed Turbulent Pipe Flow: Evolution of Experimental and Computational Methods for the Study of Turbulence
Authors: Lopez-Santana Gabriela , Kennaugh Andrew , Keshmiri Amir
Published: February 2022

Fully developed turbulent flow in a pipe was studied by considering experimental and computational methods. The aim of this work was to build on the legacy of the University of Manchester, which is widely regarded as the birthplace of turbulence due to the pioneering work of the prominent academic Professor Osborne Reynolds (1842–1912), by capturing the evolution of fluid turbulence analysis tools over the last 100 years. A classical experimental apparatus was used to measure the mean velocity field and wall shear stress through four historical techniques: static pressure drop; mean square signals measured from a hot-wire; Preston tube; and Clauser plot. Computational Fluid Dynamics (CFD) was used to simulate the pipe flow, utilizing the Reynolds-averaged Navier–Stokes (RANS) method with different two-equation turbulence models. The performance of each approach was assessed to compare the experimental and computational methods. This comparison revealed that the numerical results produced a close agreement with the experiments. The finding shows that, in some cases, CFD simulations could be used as alternative or complementary methods to experimental techniques for analyzing fully developed turbulent pipe flow.
Genome-Wide Association Study Reveals Genetic Architecture and Candidate Genes for Yield and Related Traits under Terminal Drought, Combined Heat and Drought in Tropical Maize Germplasm
Authors: Osuman Alimatu Sadia , Badu-Apraku Baffour , Karikari Benjamin , Ifie Beatrice Elohor , Tongoona Pangirayi , Danquah Eric Yirenkyi
Published: February 2022

Maize (Zea mays L.) production is constrained by drought and heat stresses. The combination of these two stresses is likely to be more detrimental. To breed for maize cultivars tolerant of these stresses, 162 tropical maize inbred lines were evaluated under combined heat and drought (CHD) and terminal drought (TD) conditions. The mixed linear model was employed for the genome-wide association study using 7834 SNP markers and several phenotypic data including, days to 50% anthesis (AD) and silking (SD), husk cover (HUSKC), and grain yield (GY). In total, 66, 27, and 24 SNPs were associated with the traits evaluated under CHD, TD, and their combined effects, respectively. Of these, four single nucleotide polymorphism (SNP) markers (SNP_161703060 on Chr01, SNP_196800695 on Chr02, SNP_195454836 on Chr05, and SNP_51772182 on Chr07) had pleiotropic effects on both AD and SD under CHD conditions. Four SNPs (SNP_138825271 (Chr03), SNP_244895453 (Chr04), SNP_168561609 (Chr05), and SNP_62970998 (Chr06)) were associated with AD, SD, and HUSKC under TD. Twelve candidate genes containing phytohormone cis-acting regulating elements were implicated in the regulation of plant responses to multiple stress conditions including heat and drought. The SNPs and candidate genes identified in the study will provide invaluable information for breeding climate smart maize varieties under tropical conditions following validation of the SNP markers.
Work-Related Health Burdens of Nurses in Germany: A Qualitative Interview Study in Different Care Settings
Authors: Lützerath Jasmin , Bleier Hannah , Schaller Andrea
Published: February 2022

Background: The growing need for nursing care is offset by a shortage of nurses, who are exposed to high physical and psychological demands in their daily work and have above-average absences that vary between different care settings. Based on the data on sick days, the question arises: What are the subjective work-related health burdens of nurses in acute care hospitals, inpatient care facilities, and outpatient care services? Methods: Sixteen semi-structured interviews were conducted in different care settings between May and September 2021. Questions about the professional career, everyday working life and personal health, violence in the workplace, and organizational framework conditions were asked. Results: The experiences of the nurses highlighted that health-related burdens have varying manifestations in different care settings. This was reflected in three main categories: health, everyday working life, and experiences of violence. In particular, the different structural framework conditions that trigger stress and the occurrence of violence are important from the perspective of the nurses. Conclusions: The results of this exploratory study can serve as a baseline for obtaining further setting-specific quantitative data that can contribute to the development, implementation, and evaluation of target group-specific health promotion programs.
Racial/Ethnic Disparities in Health and Life Insurance Denial Due to Cancer among Cancer Survivors
Authors: Lent Adrienne B. , Garrido Carlos O. , Baird Emily H. , Viela Ruta , Harris Robin B.
Published: February 2022

This study examined racial/ethnic differences in health/life insurance denial due to cancer among cancer survivors after the passage of the Affordable Care Act (ACA). Behavioral Risk Factor Surveillance System data were obtained from 2012–2020. The dependent variable asked: “Were you ever denied health insurance or life insurance coverage because of your cancer?” Cancer survivors were included if they were diagnosed with cancer after the Affordable Care Act (N = 14,815). Unadjusted and adjusted logistic regressions for age, sex, income, and employment provided odds ratios of insurance denial due to cancer across racial/ethnic groups: Non-Hispanic White, Black, and Other/mixed race; and Hispanic. Statistically significant differences (p < 0.05) were found between those who were denied or not denied insurance across sex, age, race/ethnicity, income, and employment. Adjusted regressions found significantly higher odds ratios of insurance denial for Blacks (OR: 3.00, 95% CI: 1.77, 5.08), Other/mixed race (OR: 2.16, 95% CI: 1.16, 4.02), and Hispanics (OR: 2.13, 95% CI: 1.02, 4.42) compared to Whites. Differences were observed across sex, income, and employment. Cancer survivors report racial/ethnic disparities in health and life insurance denial due to their cancer despite policy changes. This may be harmful for those who are already financially vulnerable due to their cancer diagnosis and exacerbate racial/ethnic cancer disparities.
Health Misinformation Detection in the Social Web: An Overview and a Data Science Approach
Authors: Di Sotto Stefano , Viviani Marco
Published: February 2022

The increasing availability of online content these days raises several questions about effective access to information. In particular, the possibility for almost everyone to generate content with no traditional intermediary, if on the one hand led to a process of “information democratization”, on the other hand, has negatively affected the genuineness of the information disseminated. This issue is particularly relevant when accessing health information, which impacts both the individual and societal level. Often, laypersons do not have sufficient health literacy when faced with the decision to rely or not rely on this information, and expert users cannot cope with such a large amount of content. For these reasons, there is a need to develop automated solutions that can assist both experts and non-experts in discerning between genuine and non-genuine health information. To make a contribution in this area, in this paper we proceed to the study and analysis of distinct groups of features and machine learning techniques that can be effective to assess misinformation in online health-related content, whether in the form of Web pages or social media content. To this aim, and for evaluation purposes, we consider several publicly available datasets that have only recently been generated for the assessment of health misinformation under different perspectives.
Physical Inactivity and Possible Sarcopenia in Rural Community Daycare Stations of Taiwan: A Cross-Sectional Study
Authors: Wu Yu-Zu , Loh Ching-Hui , Hsieh Jyh-Gang , Lin Shinn-Zong
Published: February 2022

Physical inactivity and possible sarcopenia pose a challenge for long-term care, especially in rural areas. We aimed to examine the prevalence of and associated factors for physical inactivity and possible sarcopenia in rural community daycare stations. A total of 275 adults aged 55–98 years (75% women) were recruited from all 11 rural community daycare stations in Northern Hualien, Taiwan. Physical inactivity was defined as less than 150 min/week of moderate-intensity aerobic physical activity. Possible sarcopenia was defined according to the Asian-specific criteria from 2019. Multiple linear and logistic regression analyses were used to determine associated factors for physical inactivity and possible sarcopenia. The prevalence of physical inactivity and possible sarcopenia was 29.1% and 68.7%, respectively. About 86.8% of possible sarcopenia were ascribed to poor five-times-sit-to-stand performance. After adjusting for covariates, poor lower-limb muscle function, e.g., slow gait speed, was associated with possible sarcopenia and physical inactivity. However, physical inactivity was not independently associated with possible sarcopenia (adjusted odds ratio 1.95, 95% confidence interval 0.88–4.30, p = 0.100). Our results indicated that individuals with poor lower-limb muscle function were more likely to have possible sarcopenia and physical inactivity. Improving lower-limb muscle function would be a priority task in rural community daycare stations.
Mobile Applications in Mood Disorders and Mental Health: Systematic Search in Apple App Store and Google Play Store and Review of the Literature
Authors: Eis Sophie , Solà-Morales Oriol , Duarte-Díaz Andrea , Vidal-Alaball Josep , Perestelo-Pérez Lilisbeth , Robles Noemí , Carrion Carme
Published: February 2022

Objectives: The main objective of this work was to explore and characterize the current landscape of mobile applications available to treat mood disorders such as depression, bipolar disorder, and dysthymia. Methods: We developed a tool that makes both the Apple App Store and the Google Play Store searchable using keywords and that facilitates the extraction of basic app information of the search results. All app results were filtered using various inclusion and exclusion criteria. We characterized all resultant applications according to their technical details. Furthermore, we searched for scientific publications on each app’s website and PubMed, to understand whether any of the apps were supported by any type of scientific evidence on their acceptability, validation, use, effectiveness, etc. Results: Thirty apps were identified that fit the inclusion and exclusion criteria. The literature search yielded 27 publications related to the apps. However, these did not exclusively concern mood disorders. 6 were randomized studies and the rest included a protocol, pilot-, feasibility, case-, or qualitative studies, among others. The majority of studies were conducted on relatively small scales and 9 of the 27 studies did not explicitly study the effects of mobile application use on mental wellbeing. Conclusion: While there exists a wealth of mobile applications aimed at the treatment of mental health disorders, including mood disorders, this study showed that only a handful of these are backed by robust scientific evidence. This result uncovers a need for further clinically oriented and systematic validation and testing of such apps.
The Influence of Environmental Polycyclic Aromatic Hydrocarbons (PAHs) Exposure on DNA Damage among School Children in Urban Traffic Area, Malaysia
Authors: Hisamuddin Nur Hazirah , Jalaludin Juliana , Abu Bakar Suhaili , Latif Mohd Talib
Published: February 2022

This study aimed to investigate the association between particulate PAHs exposure and DNA damage in Malaysian schoolchildren in heavy traffic (HT) and low traffic (LT) areas. PAH samples at eight schools were collected using a low volume sampler for 24 h and quantified using Gas Chromatography-Mass Spectrometry. Two hundred and twenty-eight buccal cells of children were assessed for DNA damage using Comet Assay. Monte-Carlo simulation was performed to determine incremental lifetime cancer risk (ILCR) and to check the uncertainty and sensitivity of the estimated risk. Total PAH concentrations in the schools in HT area were higher than LT area ranging from 4.4 to 5.76 ng m−3 and 1.36 to 3.79 ng m−3, respectively. The source diagnostic ratio showed that PAHs in the HT area is pyrogenic, mainly from diesel emission. The 95th percentile of the ILCR for children in HT and LT area were 2.80 × 10−7 and 1.43 × 10−7, respectively. The degree of DNA damage was significantly more severe in children in the HT group compared to LT group. This study shows that total indoor PAH exposure was the most significant factor that influenced the DNA damage among children. Further investigation of the relationship between PAH exposure and genomic integrity in children is required to shed additional light on potential health risks.
Compact Urban Form and Human Development: Retest Based on Heterogeneous Effects
Authors: Liu Lu , Tian Yu
Published: February 2022

The Human Development Index does not follow a normal distribution. For skewed distributions, finite mixture models can provide better estimates than fixed-effects models. In this paper, the relationship between compact cities and human development is investigated by employing a finite mixture model using panel data of Chinese prefecture-level cities. In contrast to the majority of the literature, which focuses exclusively on economic density, this article examines the impact of economic and morphological density on the level of human development. The results show that the compact development model has a negative impact on the level of human development and that the intensity of the impact varies for cities with different characteristics.
An Updated Review of Cardiovascular Events in Giant Cell Arteritis
Authors: de Boysson Hubert , Aouba Achille
Published: February 2022

Giant cell arteritis (GCA) is a systemic vasculitis with a direct and indirect increased risk of acute and chronic vascular events, affecting large and medium vessels, and responsible for most of the morbidity and mortality of this disease. We aimed in this review to provide an updated synthesis of knowledge regarding cardiovascular events observed in GCA. By definition, GCA patients are over 50 and often over 70 years old, and subsequently also present age-related cardiovascular risk factors. In addition, the systemic and vascular inflammation as well as glucocorticoids (GC) probably contribute to an accelerated atherosclerosis and to vascular changes leading to arterial stenoses and aortic dilations and/or dissections. GCA-related ischemic complications, especially ophthalmologic events, stroke or myocardial infarcts are mostly observed within the first months after the diagnosis, being mainly linked to the vasculitic process. Conversely, aortic complications, including dilations or dissections, generally occur several months or years after the diagnosis, mainly in patients with large-vessel vasculitis. In these patients, other factors such as atherosclerosis, GC-related endothelial damage and vascular wall remodeling/healing probably contribute to the vascular events. GCA management includes the detection and treatment of these previous and newly induced cardiovascular risk factors. Hence, the use of cardiovascular treatments (e.g., aspirin, anticoagulation, statins, anti-hypertensive treatments) should be evaluated individually. Aortic structural changes require regular morphologic evaluations, especially in patients with previous aortitis. The initial or secondary addition of immunosuppressants, especially tocilizumab, an anti-IL-6 receptor antibody, is discussed in patients with GCA-related cardiovascular complications and, more consensually, to limit GC-mediated comorbidities.
Improvement of Rudder Cavitation Performance Using Rudder Inflow Measurements in Large Cavitation Tunnel
Authors: Paik Bu-Geun , Jeong So-Won , Park Young-Ha , Ahn Jong-Woo , Park Ilryong , Kim Jein
Published: February 2022

One of the important things in a ship’s rudder design is the incidence angle of the rudder inflow to improve rudder cavitation performance. In the present study, the rudder inflow is measured by a 3-D LDV (laser Doppler velocimetry) system in a large cavitation tunnel, where the ship model tests are performed at a rather high Reynolds number of 107~108. Through the evaluation of the transverse velocity component using water injection experiments in the test section, the reliability of the 3-D LDV measurements is confirmed. The three-dimensional velocity components of rudder inflow between the rudder and propeller are successfully measured and are compared with numerical simulation results to see good agreement. The incidence angle distribution of the rudder inflow is obtained from the 3-D velocity components and shows a large angle at the range of 0.5 < Z/Span < 0.9, where the cavitation mainly occurred. The asymmetry-type rudder proposed based on rudder inflow measurements showed outstanding improvement of cavitation performance, compared with the flat-type rudder.
The First Population Simulation for the Zalophus japonicus (Otariidae: Sea Lions) on Dokdo, Korea
Authors: Lee Yoon-Ji , Cho Giphil , Kim Sangil , Hwang Inseo , Im Seong-Oh , Park Hye-Min , Kim Na-Yeong , Kim Myung-Joon , Lee Dasom , Kwak Seok-Nam , Lee Sang-Heon
Published: February 2022

The Japanese sea lion (Z. japonicus) has been regarded as an extinct species since the last report on Dokdo in 1951. Not much ecological information on the Z. japonicus on Dokdo (hereafter Dokdo sea lion) is currently available. Using a discrete time stage-structured population model, we reconstructed the Dokdo sea lion population to explore the effect of human hunting pressure on them. This study provides the first estimate for the Dokdo sea lion population from 1900 to 1951. The reconstructed capture numbers of the Dokdo sea lion and the parameters estimated in this study were well matched with the recorded numbers and ecological parameters reported previously for the Californian sea lion. Based on the reconstructed population, their number rapidly declined after hunting started and it took less than 10 years for a 70% decline of the initial population, which would be considered to be an extinction risk. Since some caveats exist in this study, some caution about our results is necessary. However, this study demonstrates how rapidly human over-hunting can cause the extermination of a large local population. This study will be helpful to raise people’s awareness about endangered marine animals such as local finless porpoises in Korea.