bluuebunny/crossref_metadata_2025 huggingface dataset:
Anatomical, morphological, and physiological responses of two sugarcane genotypes of contrasting susceptibility toMahanarva fimbriolata(Hemiptera: Cercopidae)
Authors: Melo C.G. , Tomaz A.C. , Soares B.O. , Kuki K.N. , Peternelli L.A. , Barbosa M.H. Pereira
Published: November 2017

The purpose of this study was to investigate and compare root morpho-anatomical traits and physiological responses of susceptible (SP81–3250) and resistant (H. Kawandang) sugarcane genotypes exposed to the attack by nymphs of spittlebugMahanarva fimbriolata(Stål) (Hemiptera: Cercopidae). Two experiments were conducted to compare the damage caused by spittlebug nymphs on fresh and dry biomass weight; lignin content in stalks; root anatomy; chlorophyll content; photosynthetic rate (A); carboxylation efficiency (A/Ci); stomatal conductance (gS) and transpiration rate (E) of these genotypes. SP81–3250 consistently obtained significantly higher damage scores than H. Kawandang in both experiments, confirming the previously observed level of resistance in each genotype. Attack by spittlebug nymphs had a much higher effect on both fresh and dry biomass weight, chlorophyll content,A, A/Ci, gsandEof SP81–3250, than that on H. Kawandang. Anatomical studies indicated the presence of aerenchyma tissue in the root cortex of SP81–3250, a feature which may facilitate penetration of the nymph's stylet into the vascular cylinder. In contrast, roots of H. Kawandang are characterized by having more dense and compact parenchyma cells. In addition, infested plants of this genotype contained an unidentified mucilaginous compound in the vascular cylinder of the roots. We conclude that resistance of H. Kawandang to spittlebug is related to the ability of this genotype to maintain normal chlorophyll content, as well as stomatal conductance and photosynthesis, thus, allowing for biomass accumulation under spittlebug attack, in contrast to SP81–3250. In addition, the presence of more compact and denser parenchymal cells, as well as that of an induced mucilaginous compound in the root's vascular cylinder, are likely to hinder host-feeding activity in nymphs, causing higher nymph mortality and therefore, reduced damage in plants of this genotype.
Short-distance dispersal of Hypothenemus hampei (Ferrari) females (Coleoptera: Curculionidae: Scolytidae) during the coffee tree fruiting period
Authors: Román-Ruiz A.K. , Ribeyre F. , Rojas J.C. , Cruz-López L. , Barrera J.F. , Dufour B.P.
Published: November 2017

The coffee berry borer (CBB), Hypothenemus hampei (Ferrari), is a multivoltine species closely associated with coffee crops worldwide, causing severe damage to the bean. In Mexico, as in all tropical regions, CBB survives during the inter-harvest period in residual berries on the ground or in dry berries remaining on the branches, and then disperses in search of the first suitable berries. In this study, we investigated how CBB dispersed from the first infested nodes during the fruiting period of Coffea canephora Pierre, which provides a favourable trophic level for this insect. Forty-five branches equally distributed in 15 coffee trees, with one infested node and four uninfested nodes, were selected. The branches were subjected to three treatments over nine weeks: 1) glue between nodes with full protection, 2) glue between nodes without protection, and 3) no glue and no protection. In addition, 45 CBB-free branches were selected and subjected to the same three treatments. CBB colonization can occur in three ways: 1) from an infested node to an uninfested node on the same branch, 2) from infested berries to uninfested berries within the nodes, 3) from branches to other branches. We also found that CBB dispersal between nodes of the same branches never occurred by walking but by flying. Thus, in this context of coffee berry development and ripening, and unlike the phenological situation of the inter-harvest period, CBB continuously travels very short distances, thus limiting its control.
Ethnic Stratification and the Equilibrium of Inequality: Ethnic Conflict in Postcolonial States
Authors: Vogt Manuel
Published: November 2017

Why are ethnic movements more likely to turn violent in some multiethnic countries than in others? Focusing on the long-term legacies of European overseas colonialism, I investigate the effect of distinct ethnic cleavage types on the consequences of ethnic group mobilization. The colonial settler states and other stratified multiethnic states are characterized by an equilibrium of inequality in which historically marginalized groups lack both the organizational strength and the opportunities for armed rebellion. In contrast, ethnic mobilization in the decolonized states and other segmented multiethnic societies is more likely to trigger violent conflict. I test these arguments in a global quantitative study from 1946 to 2009, using new data on the linguistic and religious segmentation of ethnic groups. The results confirm that the extremely unequal colonial settler states experience less violence than the decolonized states and other multiethnic countries. Ethnic conflict is generally more likely the more segmented and less hierarchically structured multiethnic states are. Specifically, stable between-group hierarchies reduce the risk of governmental conflict, whereas segmentation affects secessionist violence.
IMPACT OF SEX COMPOSITION OF LIVING CHILDREN AND COUPLES’ AGREEMENT ON SUBSEQUENT FERTILITY IN INDIA
Authors: Kastor Anshul , Chatterjee Sayantani
Published: November 2017

The desire for children could be considered a reliable predictor of subsequent fertility. At the same time, the sex composition of surviving children, along with other demographic and socioeconomic factors, may affect a couple’s fertility desire and, therefore, their subsequent fertility. This study examined the impact of the sex composition of living children and a couple’s agreement on fertility desire on their subsequent fertility in India using data came from two rounds of nationally representative surveys: the India Human Development Survey (IHDS)-I (2004–05) and IHDS-II (2011–12). To understand which factors affect the chances of an additional pregnancy or childbirth, a random effects logistic regression model was applied to the panel data. It was found that the fertility desires of both marital partners were important in determining the chances of subsequent fertility. About 35% of the couples wanting to limit children had undergone pregnancy or childbirth, while 76% of the couples wanting more children had conceived or given birth to children. In the case of discordance between the spouses, subsequent fertility was found to remain intermediate between those agreeing to continue childbirth and those wanting to limit it. The findings also affirmed that child sex preference, specifically son preference, still persists in Indian society. More than 80% of the couples with only daughters in IHDS-I mutually wanted to have additional children, whereas in families that only had sons, the chance of a subsequent pregnancy was inversely associated with the number of sons. Strong patriarchal settings, combined with cultural and socioeconomic factors, affect the persistence of sex preference in India. Programmes aimed at increasing family planning use need to address son preference and should include components that promote the value of girl children.
An Empirical Analysis of Market Segmentation on U.S. Equity Markets
Authors: Hatheway Frank , Kwan Amy , Zheng Hui
Published: November 2017

We examine the impact of trading on markets partially exempt from National Market System requirements (“dark venues”) on equity-market quality. We find evidence consistent with the notion that dark venues rely on their special features to segregate order flow based on asymmetric information risk, which results in their transactions being less informed and contributing less to price discovery on the consolidated market. Except for the execution of large transactions and trading in small stocks, the effects of dark-venue order segmentation are damaging to overall market quality. Our results have important implications for the regulation of international equity markets.
Neither obedient nor resistant: state history as cultural resource in post-genocide Rwanda
Authors: Eramian Laura
Published: November 2017

Following the 1994 genocide, scholars have criticised the Rwandan government's official account of national history and its restrictions on competing historical narratives. But what might Rwandans be doing with that state narrativebesidesconforming to it out of fear of reprisal? I argue that to understand what sustains official narratives we must grasp not only their coercive aspects, but also how social actors put them to work for different reasons. I offer four possible forms of agency in which Rwandans engage when they reproduce official history to show how – while forcibly imposed – government narratives are nonetheless cultural resources that people can turn to personal and collective visions, projects and desires. The article aims to develop a more robust understanding of how people respond to imposed narratives of nationhood and history, since it is important to attend not only to resistance, but also conformity to them.
Brokers of crisis: the everyday uncertainty of Eastern Congo's mineral négociants
Authors: Vogel Christoph , Musamba Josaphat
Published: November 2017

Eastern Democratic Republic of the Congo's (DRC) artisanal mining sector is often linked to the violent conflicts that have beset Central Africa for over two decades. While many analyses emphasise its ‘criminal’ and ‘illegal’ nature, less attention has been paid to the ambiguity of this economy, most prominently incarnated by the intermediate mineral traders callednégociants. Focusing on their entrepreneurship, networks and everyday activities, this essay offers a more nuanced understanding of local mineral trade in the context of a ‘crisis economy’ framed by competing governable orders. It investigates the uncertainty along eastern DRC's mineral supply chains, that are undergoing major regulatory changes to curb the trade of so-called ‘conflict minerals’. Drawing from extensive fieldwork, this essay demonstrates how this uncertainty shapes the négociants’ role as brokers of socio-economic life in the provinces of North and South Kivu.
Illegal trade of the Psittacidae in Venezuela
Authors: Sánchez-Mercado Ada , Asmüssen Marianne , Rodríguez Jon Paul , Moran Lisandro , Cardozo-Urdaneta Arlene , Morales Lorena Isabel
Published: November 2017

Illegal wildlife trade is one of the major threats to Neotropical psittacids, with nearly 28% of species targeted for the illegal pet trade. We analysed the most comprehensive data set on illegal wildlife trade currently available for Venezuela, from various sources, to provide a quantitative assessment of the magnitude, scope and detectability of the trade in psittacids at the national level. We calculated a specific offer index (SO) based on the frequency of which each species was offered for sale. Forty-seven species of psittacids were traded in Venezuela during 1981–2015, of which 17 were non-native. At least 641,675 individuals were traded, with an overall extraction rate of 18,334 individuals per year (35 years of accumulated reports). Amazona ochrocephala was the most frequently detected species (SO = 3.603), with the highest extraction rate (10,544 individuals per year), followed by Eupsittula pertinax (SO = 1.357) and Amazona amazonica (SO = 1.073). Amazona barbadensis, Ara ararauna and Ara chloropterus were the fourth most frequently detected species (SO = 0.564–0.615). Eleven species were involved principally in domestic trade (> 60% of records). Our approach could be the first step in developing a national monitoring programme to inform national policy on the trade in psittacids. Patterns and numbers provided may be used to update the official list of threatened species, and could also be used in planning conservation actions.
The Vulnerable fishing cat Prionailurus viverrinus and other globally threatened species in Cambodia's coastal mangroves
Authors: Thaung Ret , Herranz Muñoz Vanessa , Holden Jeremy , Willcox Daniel , Souter Nicholas J.
Published: November 2017

The Vulnerable fishing cat Prionailurus viverrinus faces a perilous future in South-east Asia. It was last sighted in Cambodia in 2003. We deployed 16 camera traps at four sites in southern Cambodia during January–May 2015 to determine if the fishing cat was still present in the country. Eight photograph/video captures of fishing cats were recorded from the mangroves in Peam Krasop Wildlife Sanctuary and one from Ream National Park, but there were no records from Botum Sakor National Park or Prey Nup. A number of other globally threatened species were also photographed in Peam Krasop Wildlife Sanctuary: the Sunda pangolin Manis javanica, the hog deer Axis porcinus and the large-spotted civet Viverra megaspila. We learnt of the killing of an alleged fishing cat at the Sanctuary in July 2015 in retaliation for raiding fishing nets. Illegal hunting and capture of fishing cats for the wildlife trade were reported by local informants at all sites. We provide photographic and video evidence of the fishing cats and highlight the importance of Cambodia's mangroves for threatened species conservation.
Diversity, prevalence and host specificity of avian parasites in southern Tunisian oases
Authors: Ayadi Tasnim , Selmi Slaheddine , Hammouda Abdessalem , Reis Sandra , Boulinier Thierry , Loiseau Claire
Published: November 2017

Oases are semi-natural woodlots surrounded by an inhospitable desert environment. This insular-like habitat system is known to support a mixture of sedentary and migratory bird species originating from different areas. However, little is known about the interactions between these birds and parasites. In this study, we investigated the diversity, prevalence and host specificity of avian haemosporidian parasites in southern Tunisian oases in two sedentary and common bird species, the laughing doveSpilopelia senegalensisand hybrid sparrowPasser domesticus × hispaniolensis, in six sites that differ regarding vegetation structure and distance to the coast. Two newHaemoproteuslineages, related to otherHaemoproteustransmitted by biting midges, were detected in doves. With regard to sparrows, all detected parasites have previously been reported in other sparrow populations, except for one newHaemoproteuslineage. Our results also showed that densely vegetated sites were characterized by the higher prevalence ofPlasmodiumbut a lower prevalence ofHaemoproteuscompared with less-vegetated sites. This is the first study aiming to explore avian parasites in the oasis habitat. Gathering data on a larger sample of oases with different sizes and isolation levels will be the next step to better understand factors shaping the transmission dynamics of avian parasites in such ecosystems.
Non-treaty Claims in Investment Treaty Arbitration
Authors: DEMIRKOL BERK
Published: November 2017

This article explores the conditions under which it is possible to bring claims based on non-international investment agreement (IIA) norms of international law in investment treaty arbitration. For that purpose, it analyzes in the first instance broad dispute settlement clauses incorporated in IIAs that make reference to the settlement of ‘any investment dispute’. Such clauses grant jurisdiction to investment treaty tribunals to hear non-IIA claims. However, at least two additional conditions need to be satisfied for the investor to bring a self-standing claim based on a non-IIA norm of international law. First, the non-IIA instrument (a contract or another international treaty) may include a dispute settlement clause envisaging exclusive jurisdiction in favour of another forum. Second, the investor's standing to bring a claim based on a non-IIA norm of international law depends on whether this norm attributes any legal entitlement in the benefit of the investor.
Estimates of the burden of illness for eight enteric pathogens associated with animal contact in Canada
Authors: MURRAY R. , TATARYN J. , PINTAR K. , THOMAS M. K.
Published: November 2017

Enteric pathogens are commonly known to be transmitted through food or water; however, contact with animals is another important transmission route. This study estimated the annual burden of illness attributable to animal contact for eight enteric pathogens in Canada. Using data from a Canadian expert elicitation on transmission routes, the proportion of enteric illnesses attributable to animal contact was estimated for each pathogen to estimate the annual number of illnesses, hospitalizations and deaths in Canada. For each estimate, a mean and probability intervals were generated. Of all illnesses caused by these eight pathogens, 16% were estimated attributable to animal contact. This estimate translates to 86 000 (31 000–166 000) illnesses, 488 (186–890) hospitalizations and 12 (2–28) deaths annually for the eight pathogens combined.Campylobacterspp. is the leading cause of illnesses annually, with an estimated 38 000 (14 000–71 000) illnesses occurring each year, followed by non-typhoidalSalmonellaspp. (17 000, 6000–32 000). The majority of hospitalizations were attributable to non-typhoidalSalmonellaspp. (36%) andCampylobacterspp. (31%). Non-typhoidalSalmonellaspp. (28%) andListeria monocytogenes(31%) were responsible for the majority of the estimated deaths. These results identify farm animal and pet/pet food exposure as key pathways of transmission for several pathogens. The estimated burden of illness associated with animal contact is substantial.
Seasonality of urinary tract infections in the United Kingdom in different age groups: longitudinal analysis of The Health Improvement Network (THIN)
Authors: ROSELLO A. , POUWELS K. B. , DOMENECH DE CELLÈS M. , VAN KLEEF E. , HAYWARD A. C. , HOPKINS S. , ROBOTHAM J. V. , SMIESZEK T. , OPATOWSKI L. , DEENY S. R.
Published: November 2017

Evidence regarding the seasonality of urinary tract infection (UTI) consultations in primary care is conflicting and methodologically poor. To our knowledge, this is the first study to determine whether this seasonality exists in the UK, identify the peak months and describe seasonality by age. The monthly number of UTI consultations (N = 992 803) and nitrofurantoin and trimethoprim prescriptions (N = 1 719 416) during 2008–2015 was extracted from The Health Improvement Network (THIN), a large nationally representative UK dataset of electronic patient records. Negative binomial regression models were fitted to these data to investigate seasonal fluctuations by age group (14–17, 18–24, 25–45, 46–69, 70–84, 85+) and by sex, accounting for a change in the rate of UTI over the study period. A September to November peak in UTI consultation incidence was observed for ages 14–69. This seasonality progressively faded in older age groups and no seasonality was found in individuals aged 85+, in whom UTIs were most common. UTIs were rare in males but followed a similar seasonal pattern than in females. We show strong evidence of an autumnal seasonality for UTIs in individuals under 70 years of age and a lack of seasonality in the very old. These findings should provide helpful information when interpreting surveillance reports and the results of interventions against UTI.
PhD Abstracts
Authors: HUTTON GRAHAM
Published: November 2017

Many students complete PhDs in functional programming each year. As a service to the community, the Journal of Functional Programming publishes the abstracts from PhD dissertations completed during the previous year.
Prothymosin alpha expression in the vertebrate testis: a comparative review
Authors: Venditti Massimo , Minucci Sergio
Published: November 2017

Prothymosin alpha (PTMA) is a highly acidic, intrinsically disordered protein that was first extracted from rat thymus and characterized as an immunogenic factor but soon detected in a variety of mammalian tissues. The presence of a nuclear localization signal and the adoption of a peculiar random-coil conformation are among the reasons behind its interaction with several molecular partners, hence at this time PTMA is known to be a very conserved and widely expressed molecule, involved in numerous and diverse biological processes. Only few studies have tried to weigh its possible involvement in reproduction, specifically in male gametogenesis: first reports have suggested that PTMA might be associated with the proliferative and early-meiotic phases of mammal spermatogenesis. Some years later, a comparative project on vertebrate spermatogenesis reported the isolation, for the first time, of prothymosin in a non-mammalian species, the amphibianPelophylax esculentus. PTMA transcript and protein are localized in the germinal compartment, from spermatocytes to spermatozoa. A congruent pattern has been highlighted in studies on the fishTorpedo marmorataandDanio rerio, and in the mammalRattus norvegicus, in which the expression of PTMA has been found in meiotic and post-meiotic germ cells inside testicular cysts and tubules. Moreover, its presence has been confirmed in rat and human spermatozoa (associated with the acrosome); its retention in the apical region of the head after the acrosome reaction revealed a striking conservation of the pattern during phylogenesis and suggested a possible role for the protein in gametogenesis and in fertilization.
Pulsar positioning system: a quest for evidence of extraterrestrial engineering
Authors: Vidal Clément
Published: November 2017

Pulsars have at least two impressive applications. First, they can be used as highly accurate clocks, comparable in stability to atomic clocks; secondly, a small subset of pulsars, millisecond X-ray pulsars, provide all the necessary ingredients for a passive galactic positioning system. This is known in astronautics as X-ray pulsar-based navigation (XNAV). XNAV is comparable to GPS, except that it operates on a galactic scale. I propose a SETI-XNAV research program to test the hypothesis that this pulsar positioning system might be an instance of galactic-scale engineering by extraterrestrial beings. The paper starts by exposing the basics of pulsar navigation, continues with a critique of the rejection of the extraterrestrial hypothesis when pulsars were first discovered. The core section of the paper proposes lines of inquiry for SETI-XNAV, related to the pulsar distribution and power in the galaxy; their population; their evolution; possible pulse synchronizations; pulsar usability when navigating near the speed of light; decoding galactic coordinates; directed panspermia; and information content in pulses. Even if pulsars are natural, they are likely to be used as standards by ETIs in the galaxy. I discuss possible objections and potential benefits for humanity, whether the research program succeeds or not.
Editorial for Special Issue: Complexity within Cognitive Behaviour Therapy
Authors: Lomax Claire , Barton Stephen
Published: November 2017

As therapists we frequently use and hear the term ‘complexity’ in relation to clients, situations and settings. Although we may assume there is a shared understanding of what is meant by complexity, is this true? Do we really know what we mean by describing someone, or something, as complex? If we define complexity as ‘consisting of many different and connected parts, not easy to analyse or understand’ (Oxford English Dictionary, 2017), then we are probably describing intersections and interactions between different elements that can influence each other. Interestingly, the origin of the term derives from the Latin past participle plexus, meaning braided or entwined, which captures neatly the sense of the term ‘complex’ as meaning literally braided together. The breadth of this definition therefore may help to account for the diversity of the ways in which the term complexity is used in clinical settings. Continuing with the idea of the plait or braid, it also gives a sense of the number of threads or strands that could be incorporated within such a system. Complexity can derive from any source, and can interact with any part, so it can derive from the patient, the therapist, the therapeutic relationship or the healthcare setting; and each of these may interact with one or more parts. So from any source, complexity can affect processes and outcomes of care.
Socialization to the model in adolescent cognitive behavioural therapy: measurement and insights
Authors: Mahoney-Davies Gerwyn , Roberts-Collins Cara , Russell Ailsa , Loades Maria
Published: November 2017

Socializing a client to the cognitive behavioural model is advised in almost every cognitive behavioural therapy (CBT) textbook, but there is limited evidence for whether socialization is measurable or important. The aim of the study was to pilot a written and interview-based measure of socialization to investigate whether socialization to the model can be measured in a sample of young people who have completed CBT. Sixteen participants (mean age 14.9 years, 75% female) completed a semi-structured socialization interview and a novel written measure of socialization. Treating clinicians were asked to provide subjective ratings of participant socialization. The structure and content of these measures was examined. A moderate but non-significant correlation was found between the novel written measure of socialization and clinician rating of socialization (r = .37). The concept of ‘socialization’ is not well understood and the socialization interview presented mixed, unclear results. This may be due to issues with the design, but may also be that socialization, as currently understood, is more complex than can be captured in this way. The important aspect of this study is introducing the concept of measuring socialization and factors that may be important in future research. Socialization to the model is an important construct within CBT but at present is a challenging concept to measure. Future research will need to focus on operationalizing the concept further and refining measures so that it can be accurately captured. Understanding which therapist and client behaviours contribute to the process of socialization could conceivably improve outcomes, but this cannot be done until this area is understood more fully.
TDOA-based microwave imaging algorithm for real-time microwave ablation monitoring
Authors: Kidera Shouhei , Neira Luz Maria , Van Veen Barry D. , Hagness Susan C.
Published: November 2017

Microwave ablation is widely recognized as a promising minimally invasive tool for treating cancer. Real-time monitoring of the dimensions of the ablation zone is indispensable for ensuring an effective and safe treatment. In this paper, we propose a microwave imaging algorithm for monitoring the evolution of the ablation zone. Our proposed algorithm determines the boundary of the ablation zone by exploiting the time difference of arrival (TDOA) between signals received before and during the ablation at external antennas surrounding the tissue, using the interstitial ablation antenna as the transmitter. A significant advantage of this method is that it requires few assumptions about the dielectric properties of the propagation media. Also the simplicity of the signal processing, wherein the TDOA is determined from a cross-correlation calculation, allows real-time monitoring and provides robust performance in the presence of noise. We investigate the performance of this approach for the application of breast tumor ablation. We use simulated array measurements obtained from finite-difference time-domain simulations of magnetic resonance imaging-derived numerical breast phantoms. The results demonstrate that our proposed method offers the potential to achieve millimeter-order accuracy and real-time operation in estimating the boundary of the ablation zone in heterogeneous and dispersive breast tissue.
The Mere Exposure Instruction Effect
Authors: Van Dessel Pieter , Mertens Gaëtan , Smith Colin Tucker , De Houwer Jan
Published: November 2017

Abstract. The mere exposure effect refers to the well-established finding that people evaluate a stimulus more positively after repeated exposure to that stimulus. We investigated whether a change in stimulus evaluation can occur also when participants are not repeatedly exposed to a stimulus, but are merely instructed that one stimulus will occur frequently and another stimulus will occur infrequently. We report seven experiments showing that (1) mere exposure instructions influence implicit stimulus evaluations as measured with an Implicit Association Test (IAT), personalized Implicit Association Test (pIAT), or Affect Misattribution Procedure (AMP), but not with an Evaluative Priming Task (EPT), (2) mere exposure instructions influence explicit evaluations, and (3) the instruction effect depends on participants’ memory of which stimulus will be presented more frequently. We discuss how these findings inform us about the boundary conditions of mere exposure instruction effects, as well as the mental processes that underlie mere exposure and mere exposure instruction effects.
The histone code reader Spin1 controls skeletal muscle development
Authors: Greschik Holger , Duteil Delphine , Messaddeq Nadia , Willmann Dominica , Arrigoni Laura , Sum Manuela , Jung Manfred , Metzger Daniel , Manke Thomas , Günther Thomas , Schüle Roland
Published: November 2017

While several studies correlated increased expression of the histone code reader Spin1 with tumor formation or growth, little is known about physiological functions of the protein. We generated Spin1M5 mice with ablation of Spin1 in myoblast precursors using the Myf5-Cre deleter strain. Most Spin1M5 mice die shortly after birth displaying severe sarcomere disorganization and necrosis. Surviving Spin1M5 mice are growth-retarded and exhibit the most prominent defects in soleus, tibialis anterior, and diaphragm muscle. Transcriptome analyses of limb muscle at embryonic day (E) 15.5, E16.5, and at three weeks of age provided evidence for aberrant fetal myogenesis and identified deregulated skeletal muscle (SkM) functional networks. Determination of genome-wide chromatin occupancy in primary myoblast revealed direct Spin1 target genes and suggested that deregulated basic helix-loop-helix transcription factor networks account for developmental defects in Spin1M5 fetuses. Furthermore, correlating histological and transcriptome analyses, we show that aberrant expression of titin-associated proteins, abnormal glycogen metabolism, and neuromuscular junction defects contribute to SkM pathology in Spin1M5 mice. Together, we describe the first example of a histone code reader controlling SkM development in mice, which hints at Spin1 as a potential player in human SkM disease.
Doxycycline induces apoptosis via ER stress selectively to cells with a cancer stem cell-like properties: importance of stem cell plasticity
Authors: Matsumoto Takashi , Uchiumi Takeshi , Monji Keisuke , Yagi Mikako , Setoyama Daiki , Amamoto Rie , Matsushima Yuichi , Shiota Masaki , Eto Masatoshi , Kang Dongchon
Published: November 2017

Tumor heterogeneity can be traced back to a small subset of cancer stem cells (CSCs), which can be derived from a single stem cell and show chemoresistance. Recent studies showed that CSCs are sensitive to mitochondrial targeting antibiotics such as doxycycline. However, little is known about how cancer cells undergo sphere formation and how antibiotics inhibit CSC proliferation. Here we show that under sphere-forming assay conditions, prostate cancer cells acquired CSC-like properties: promoted mitochondrial respiratory chain activity, expression of characteristic CSC markers and resistance to anticancer agents. Furthermore, those CSC-like properties could reversibly change depending on the culture conditions, suggesting some kinds of CSCs have plasticity in tumor microenvironments. The sphere-forming cells (i.e. cancer stem-like cells) showed increased contact between mitochondria and mitochondrial associated-endoplasmic reticulum (ER) membranes (MAM). Mitochondrial targeting doxycycline induced activating transcription factor 4 (ATF4) mediated expression of ER stress response and led to p53-upregulated modulator of apoptosis (PUMA)-dependent apoptosis only in the cancer stem-like cells. We also found that doxycycline effectively suppressed the sphere formation in vitro and blocked CD44v9-expressing tumor growth in vivo. In summary, these data provide new molecular findings that monolayer cancer cells acquire CSC-like properties in a reversible manner. These findings provide important insights into CSC biology and a potential new treatment of targeting mitochondria dependency.
HUWE1 variants cause dominant X-linked intellectual disability: a clinical study of 21 patients
Authors: Moortgat Stéphanie , Berland Siren , Aukrust Ingvild , Maystadt Isabelle , Baker Laura , Benoit Valerie , Caro-Llopis Alfonso , Cooper Nicola S. , Debray François-Guillaume , Faivre Laurence , Gardeitchik Thatjana , Haukanes Bjørn I. , Houge Gunnar , Kivuva Emma , Martinez Francisco , Mehta Sarju G. , Nassogne Marie-Cécile , Powell-Hamilton Nina , Pfundt Rolph , Rosello Monica , Prescott Trine , Vasudevan Pradeep , van Loon Barbara , Verellen-Dumoulin Christine , Verloes Alain , Lippe Charlotte von der , Wakeling Emma , Wilkie Andrew O. M. , Wilson Louise , Yuen Amy , Study DDD , Low Karen J. , Newbury-Ecob Ruth A.
Published: November 2017

Whole-gene duplications and missense variants in the HUWE1 gene (NM_031407.6) have been reported in association with intellectual disability (ID). Increased gene dosage has been observed in males with non-syndromic mild to moderate ID with speech delay. Missense variants reported previously appear to be associated with severe ID in males and mild or no ID in obligate carrier females. Here, we report the largest cohort of patients with HUWE1 variants, consisting of 14 females and 7 males, with 15 different missense variants and one splice site variant. Clinical assessment identified common clinical features consisting of moderate to profound ID, delayed or absent speech, short stature with small hands and feet and facial dysmorphism consisting of a broad nasal tip, deep set eyes, epicanthic folds, short palpebral fissures, and a short philtrum. We describe for the first time that females can be severely affected, despite preferential inactivation of the affected X chromosome. Three females with the c.329 G  >  A p.Arg110Gln variant, present with a phenotype of mild ID, specific facial features, scoliosis and craniosynostosis, as reported previously in a single patient. In these females, the X inactivation pattern appeared skewed in favour of the affected transcript. In summary, HUWE1 missense variants may cause syndromic ID in both males and females.
Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair
Authors: Dogra Deepika , Ahuja Suchit , Kim Hyun-Taek , Rasouli S. Javad , Stainier Didier Y. R. , Reischauer Sven
Published: November 2017

Zebrafish regenerate damaged myocardial tissue very effectively. Hence, insights into the molecular networks underlying zebrafish heart regeneration might help develop alternative strategies to restore human cardiac performance. While TGF-β signaling has been implicated in zebrafish cardiac regeneration, the role of its individual ligands remains unclear. Here, we report the opposing expression response during zebrafish heart regeneration of two genes, mstnb and inhbaa, which encode TGF-β family ligands. Using gain-of-function (GOF) and loss-of-function (LOF) approaches, we show that these ligands mediate inverse effects on cardiac regeneration and specifically on cardiomyocyte (CM) proliferation. Notably, we find that Inhbaa functions as a CM mitogen and that its overexpression leads to accelerated cardiac recovery and scar clearance after injury. In contrast, mstnb GOF and inhbaa LOF both lead to unresolved scarring after cardiac injury. We further show that Mstnb and Inhbaa inversely control Smad2 and Smad3 transcription factor activities through alternate Activin type 2 receptors.
Synergistic gene expression during the acute phase response is characterized by transcription factor assisted loading
Authors: Goldstein Ido , Paakinaho Ville , Baek Songjoon , Sung Myong-Hee , Hager Gordon L.
Published: November 2017

The cytokines interleukin 1β and 6 (IL-1β, IL-6) mediate the acute phase response (APR). In liver, they regulate the secretion of acute phase proteins. Using RNA-seq in primary hepatocytes, we show that these cytokines regulate transcription in a bifurcated manner, leading to both synergistic and antagonistic gene expression. By mapping changes in enhancer landscape and transcription factor occupancy (using ChIP-seq), we show that synergistic gene induction is achieved by assisted loading of STAT3 on chromatin by NF-κB. With IL-6 treatment alone, STAT3 does not efficiently bind 20% of its coordinated binding sites. In the presence of IL-1β, NF-κB is activated, binds a subset of enhancers and primes their activity, as evidenced by increasing H3K27ac. This facilitates STAT3 binding and synergistic gene expression. Our findings reveal an enhancer-specific crosstalk whereby NF-κB enables STAT3 binding at some enhancers while perturbing it at others. This model reconciles seemingly contradictory reports of NF-κB-STAT3 crosstalk.
Chiral transport along magnetic domain walls in the quantum anomalous Hall effect
Authors: Rosen Ilan T. , Fox Eli J. , Kou Xufeng , Pan Lei , Wang Kang L. , Goldhaber-Gordon David
Published: November 2017

The quantum anomalous Hall effect in thin film magnetic topological insulators (MTIs) is characterized by chiral, one-dimensional conduction along the film edges when the sample is uniformly magnetized. This has been experimentally confirmed by measurements of quantized Hall resistance and near-vanishing longitudinal resistivity in magnetically doped (Bi,Sb)2Te3. Similar chiral conduction is expected along magnetic domain walls, but clear detection of these modes in MTIs has proven challenging. Here, we intentionally create a magnetic domain wall in an MTI, and study electrical transport along the domain wall. In agreement with theoretical predictions, we observe chiral transport along a domain wall. We present further evidence that two modes equilibrate while co-propagating along the length of the domain wall.
Hierarchically porous, and Cu- and Zn-containing γ-AlOOH mesostrands as adjuvants for cancer immunotherapy
Authors: Li Xia , Shenashen Mohamed A. , Wang Xiupeng , Ito Atsuo , Taniguchi Akiyoshi , EI-Safty Sherif A.
Published: November 2017

Alum is the only licensed adjuvant by Food and Drug Administration of USA used in many human vaccines and has excellent safety record in clinical applications. However, alum hardly induces T helper 1 (Th1) immune responses that are required for anti-tumor immunity. In the present study, we fabricated hierarchical copper- and zinc- buds dressing γ-AlOOH mesostrands (Cu- and Zn-AMSs) with randomly wrinkled morphology, mesoscale void- or cave-like pockets, high-exposed surface coverage sites, and positive charge streams in saline. We confirmed that Cu- and Zn-AMSs promoted intracellular uptake of model cancer antigen (ovalbumin, OVA) by THP-1-differentiated macrophage-like cellsin vitro. Moreover, Cu- and Zn-AMSs enhanced maturation and cytokine release of bone marrow dendritic cellsin vitro.In vivostudy demonstrated that Cu- and Zn-AMSs markedly induced anti-tumor-immunity and enhanced CD4+and CD8+T cell populations in splenocytes of mice. These findings demonstrated that hierarchical copper- and zinc- buds dressing γ-AlOOH mesostrands, which are oriented in randomly wrinkled matrice, are suitable platforms as novel adjuvants for cancer immunotherapy.
Tabby graphene: Dimensional magnetic crossover in fluorinated graphite
Authors: Makarova T. L. , Shelankov A. L. , Shames A. I. , Zyrianova A. A. , Komlev A. A. , Chekhova G. N. , Pinakov D. V. , Bulusheva L. G. , Okotrub A. V. , Lähderanta E.
Published: November 2017

Tabby is a pattern of short irregular stripes, usually related to domestic cats. We have produced Tabby patterns on graphene by attaching fluorine atoms running as monoatomic chains in crystallographic directions. Separated by non-fluorinated sp2 carbon ribbons, sp3-hybridized carbon atoms bonded to zigzag fluorine chains produce sp2-sp3 interfaces and spin-polarized edge states localized on both sides of the chains. We have compared two kinds of fluorinated graphite samples C2F
x
, with x near to 1 and x substantially below 1. The magnetic susceptibility of C2F
x
(x < 1) shows a broad maximum and a thermally activated spin gap behaviour that can be understood in a two-leg spin ladder model with ferromagnetic legs and antiferromagnetic rungs; the spin gap constitutes about 450 K. Besides, stable room-temperature ferromagnetism is observed in C2F
x
(x < 1) samples: the crossover to a three-dimensional magnetic behaviour is due to the onset of interlayer interactions. Similarly prepared C2F
x
(x ≈ 1) samples demonstrate features of two-dimensional magnetism without signs of high-temperature magnetic ordering, but with transition to a superparamagnetic state below 40 K instead. The magnetism of the Tabby graphene is stable until 520 K, which is the temperature of the structural reconstruction of fluorinated graphite.
DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling
Authors: Badawy Shaymaa Mohamed Mohamed , Okada Taro , Kajimoto Taketoshi , Ijuin Takeshi , Nakamura Shun-ichi
Published: November 2017

Sphingosine 1-phosphate (S1P) is a pleiotropic lipid mediator involved in the regulation of immune cell trafficking and vascular permeability acting mainly through G-protein-coupled S1P receptors (S1PRs). However, mechanism underlying how S1PRs are coupled with G-proteins remains unknown. Here we have uncovered that palmitoylation of a prototypical subtype S1P1R is prerequisite for subsequent inhibitory G-protein (Gi) coupling. We have identified DHHC5 as an enzyme for palmitoylation of S1P1R. Under basal conditions, S1P1R was functionally associated with DHHC5 in the plasma membranes (PM) and was fully palmitoylated, enabling Gi coupling. Upon stimulation, the receptor underwent internalisation leaving DHHC5 in PM, resulting in depalmitoylation of S1P1R. We also revealed that while physiological agonist S1P-induced endocytosed S1P1R readily recycled back to PM, pharmacological FTY720-P-induced endocytosed S1P1R-positive vesicles became associated with DHHC5 in the later phase, persistently transmitting Gi signals there. This indicates that FTY720-P switches off the S1P signal in PM, while switching on its signal continuously inside the cells. We propose that DHHC5-mediated palmitoylation of S1P1R determines Gi coupling and its signalling in a spatio/temporal manner.
Evolving autonomous learning in cognitive networks
Authors: Sheneman Leigh , Hintze Arend
Published: November 2017

There are two common approaches for optimizing the performance of a machine: genetic algorithms and machine learning. A genetic algorithm is applied over many generations whereas machine learning works by applying feedback until the system meets a performance threshold. These methods have been previously combined, particularly in artificial neural networks using an external objective feedback mechanism. We adapt this approach to Markov Brains, which are evolvable networks of probabilistic and deterministic logic gates. Prior to this work MB could only adapt from one generation to the other, so we introduce feedback gates which augment their ability to learn during their lifetime. We show that Markov Brains can incorporate these feedback gates in such a way that they do not rely on an external objective feedback signal, but instead can generate internal feedback that is then used to learn. This results in a more biologically accurate model of the evolution of learning, which will enable us to study the interplay between evolution and learning and could be another step towards autonomously learning machines.
Risk of mortality during and after the 2011 Great East Japan Earthquake and Tsunami among older coastal residents
Authors: Aida Jun , Hikichi Hiroyuki , Matsuyama Yusuke , Sato Yukihiro , Tsuboya Toru , Tabuchi Takahiro , Koyama Shihoko , Subramanian S. V. , Kondo Katsunori , Osaka Ken , Kawachi Ichiro
Published: November 2017

The Japan Gerontological Evaluation Study is a nationwide cohort study of individuals aged 65 years and older established in July 2010. Seven months later, one of the study field sites was directly in the line of the 2011 Great East Japan Earthquake and Tsunami. Despite the 1-hour warning interval between the earthquake and tsunami, many coastal residents lost their lives. We analyzed the risk of all-cause mortality on the day of the disaster as well as in the 38-month interval after the disaster. Among 860 participants, 33 (3.8%) died directly because of the tsunami and an additional 95 people died during the 38-month follow-up period. Individuals with depressive symptoms had elevated risk of mortality on the day of the disaster (odds ratio = 3.90 [95% CI: 1.13, 13.47]). More socially connected people also suffered increased risk of mortality, although these estimates were not statistically significant. In contrast, after the disaster, frequent social interactions reverted back to predicting improved survival (hazard ratio = 0.46 (95% CI: 0.26, 0.82)). Depressive symptoms and stronger social connectedness were associated with increased risk of mortality on the day of the disaster. After the disaster, social interactions were linked to improved survival.
Estrogen receptor 1 (ESR1) regulates VEGFA in adipose tissue
Authors: Fatima L. A. , Campello R. S. , Santos R. de Souza , Freitas H. S. , Frank A. P , Machado U. F. , Clegg D. J.
Published: November 2017

Vascular endothelial growth factor A (VEGFA) is a key factor in the regulation of angiogenesis in adipose tissue. Poor vascularization during adipose tissue proliferation causes fibrosis and local inflammation, and is associated with insulin resistance. It is known that 17-beta estradiol (E2) regulates adipose tissue function and VEGFA expression in other tissues; however, the ability of E2 to regulate VEGFA in adipose tissue is currently unknown. In this study, we showed that, in 3T3-L1 cells, E2 and the estrogen receptor 1 (ESR1) agonist PPT induced VEGFA expression, while ESR1 antagonist (MPP), and selective knockdown of ESR1 using siRNA decreased VEGFA and prevented the ability of E2 to modulate its expression. Additionally, we found that E2 and PPT induced the binding of hypoxia inducible factor 1 alpha subunit (HIF1A) in the VEGFA gene promoter. We further found that VEGFA expression was lower in inguinal and gonadal white adipose tissues of ESR1 total body knockout female mice compared to wild type mice. In conclusion, our data provide evidence of an important role for E2/ESR1 in modulating adipose tissue VEGFA, which is potentially important to enhance angiogenesis, reduce inflammation and improve adipose tissue function.
Consistency and flexibility in solving spatial tasks: different horses show different cognitive styles
Authors: Baragli Paolo , Vitale Valentina , Sighieri Claudio , Lanata Antonio , Palagi Elisabetta , Reddon Adam R.
Published: November 2017

Individual animals vary in their behaviour and reactions to novel situations. These differences may extend to differences in cognition among individuals. We tested twenty-six horses for their ability to detour around symmetric and asymmetric obstacles. All of the animals were able to get around the barrier to reach a food target, but varied in their approach. Some horses moved slowly but were more accurate in choosing the shortest way. Other horses acted quickly, consistently detoured in the same direction, and did not reliably choose the shortest way. The remaining horses shifted from a faster, directionally consistent response with the symmetric barrier, to a slower but more accurate response with the asymmetric barrier. The asymmetric barrier induced a reduction in heart rate variability, suggesting that this is a more demanding task. The different approaches used to solve the asymmetric task may reflect distinct cognitive styles in horses, which vary among individuals, and could be linked to different personality traits. Understanding equine behaviour and cognition can inform horse welfare and management.