bluuebunny/crossref_metadata_2025 huggingface dataset:
Sleep disturbance has the largest impact on children's behavior and emotions
Authors: Matsuoka Michiko , Matsuishi Toyojiro , Nagamitsu Shinichiro , Iwasaki Mizue , Iemura Akiko , Obara Hitoshi , Yamashita Yushiro , Maeda Masaharu , Kakuma Tatsuyuki , Uchimura Naohisa
Published: November 2022

Objective

Children's behavior and emotions are affected by sleep disturbances, the parent-child relationship, media viewing time, and the social status of parents and caregivers. We conducted a questionnaire survey to identify the factors that have the greatest impact on children's behavior and emotions and how these factors relate to each other.

Methods

A parental questionnaire survey was performed at a public elementary school. The questionnaire comprised questions on the family environment (e.g., family structure, media and game exposure, after-school lessons, and caregiver's work schedule) and physical information, the Strengths and Difficulties Questionnaire (SDQ), the Children's Sleep Habits Questionnaire (CSHQ), and the Pittsburgh Sleep Quality Index (PSQI) for parents' sleep condition. A path diagram was drawn to hypothesize the complex interrelationships among factors, and structural equation modeling was used to estimate the path coefficients.

Result

We identified several factors that significantly affected the SDQ score. The CSHQ total score had the largest impact, followed by after-school lessons, single-mother families, and children's sex. In addition, several indirect pathways that led to the CSHQ score (i.e., a pathway from time spent watching television to CSHQ score via children's bedtime and a pathway from single-mother family to CSHQ score via PSQI total score) significantly affected the SDQ score.

Conclusion

Children's sleep habits that were influenced by several environmental factors had the greatest impact on children's behavior and emotions, which suggested that children's behavioral problems can be improved by interventions focused on sleep habits, such as sleep hygiene instructions.
Chronic effects of different exercise types on brain activity in healthy older adults and those with Parkinson’s disease: A systematic review
Authors: Wang Leilei , Li Feiyue , Tang Lu
Published: November 2022

Objective: This study aimed to compare the regulation of brain activity by different kinds of long-term exercises (Tai Chi, treadmill training, and dancing) in healthy older adults and those with PD.

Methods: From January 2000 to October 2021, the electronic databases PubMed, Web of Science, and Scopus were searched. All articles were screened throughout the inclusion and exclusion criteria, which was followed by PICOS criteria. Finally, all articles were systematically reviewed with analyses.

Results: 29 studies were identified for this review, 24 of which were finally included in a group of healthy older adults, and five of which in a group of people with PD. All studies showed that significant changes were showed on people with PD and healthy older adults’ brain activity after three terms of the exercises we chose. An inverse change trend on the functional connectivity in people with PD was observed after treadmill training, whereas increased brain activity, cognitive function, memory, and emotion were noticed in healthy older adults.

Conclusion: Our findings suggest that different patterns of brain activity were also observed between healthy older adults and people with PD after treadmill training. However, more robust evidence and comprehensive studies are needed to determine if there is a difference between healthy older adults and people with PD.
Spraying sorbitol-chelated calcium affected foliar calcium absorption and promoted the yield of peanut (Arachis hypogaea L.)
Authors: Li Tengsheng , Wei Qianqian , Sun Wei , Tan Huiting , Cui Yuzhao , Han Chuanhao , Zhang Huanyang , Zeng Fanhe , Huang Mingli , Yan Dongyun
Published: November 2022

The prevalent use of foliar calcium fertilizers in peanut production is inorganic, but calcium absorbed from the foliar has poor availability. Sorbitol-chelated calcium is a novel organic foliar calcium fertilizer that has rarely been studied for application in peanut production. To explore whether calcium absorption and peanut yields can be affected by foliar application of sorbitol-chelated calcium, this study conducted two field experiments using Virginia peanut (Huayu-22) in 2020 and 2021. The five spray treatments included: deionized water (CK), sorbitol (Sor), calcium nitrate (CaN), a mixture of sorbitol and calcium nitrate (SN), and sorbitol-chelated calcium (SC). The yield of peanuts treated with sorbitol-chelated calcium was increased by 12.31-16.63%, 10.22-11.83%, 6.31-9.69%, and 4.18-6.99% compared to the CK, Sor, CaN, and SN treatments, respectively. Sorbitol-chelated calcium had the lowest contact angle due to the wetting effect of sorbitol, which promoted calcium absorption by leaves. Sorbitol-chelated calcium improved the leaf calcium concentration by 13.12-19.32% and kernel calcium concentration by 6.49-8.15% compared to the CK treatment. Foliar fertilization increased the calcium concentration of each subcellular fraction of leaves and changed the distribution of calcium in mesophyll cells. This change was directly observed by transmission electron microscopy. Additionally, spraying sorbitol alone obtained similar effects to spraying calcium nitrate alone, indicating that the benefits of sorbitol itself were not negligible. The results of the principal component and correlation analysis showed that the increase in calcium concentrations and the change in calcium distribution improved the pod traits of the peanut, thus affecting the peanut yield. The above results showed that from the perspective of calcium absorption and distribution, sorbitol-chelated calcium is a more effective foliar calcium fortifier for peanuts and effectively improves peanut yields.
Psychosocial safety climate (PSC) at middle management level in the healthcare sector: A contribution to the Italian validation of psychosocial safety climate-4
Authors: Fattori Alice , Comotti Anna , Bordini Lorenzo , Dollard Maureen F. , Bonzini Matteo
Published: November 2022

Introduction

Psychosocial safety climate (PSC) refers to workers’ shared perceptions of organizational policies, practices and procedures for the protection of psychological health and safety. PSC offers a multilevel organizational approach that expands traditional models of workplace stress, giving a more comprehensive understanding of occupational health and safety issues. Although considerable research on psychosocial risks in the healthcare sector has been conducted, few studies have explored the role of PSC among healthcare workers at middle management level. Additionally, no validated version of PSC is available in Italian language. The aim of this study is to contribute to the validation of the Italian 4-item version of the PSC and to explore this theory within the Job Demands-Resources model (JD-R) among a sample of Italian healthcare workers by testing PSC at the middle management level.

Methods

We used cross-sectional data from 276 employees working in 17 different wards in a large Italian hospital. Intra-class coefficient (ICC) coefficient and agreement index were used to test PSC as a climate construct (data nested to hospital ward level). We performed hierarchical linear models to test mediation and moderation effects.

Results

The Italian version of PSC-4 proved to have good psychometric properties and confirmed its role as a group-level construct (α = 0.84; ICC = 0.16). Multilevel random coefficient models showed PSC was associated with Job demands (Effort: B = −0.36, SE = 0.07; Emotional demands: B = −0.03, SE = 0.01) and Job resources (Reward: B = 1.16, SE = 0.01; Physical work environment: B = 0.06, SE = 0.01). Results confirmed the indirect effect of PSC on Psychological (Burnout) and Occupational health (Job satisfaction) outcomes supporting the role of Job resources and Job demands as mediators. The multilevel analysis did not find a significant interaction terms between PSC and Job demands on Burnout therefore the moderation hypothesis was not supported.

Discussion

The Italian version of PSC-4 is a valid tool to evaluate PSC. These findings sustain the multilevel framework of PSC and the significant role played by mid-leaders in both the health impairment and motivational path. Further studies should explore the buffering effect of PSC at higher baseline levels as well as the adoption of PSC as a target for occupational health intervention the Italian context.
Mediation effects of post-series depression on the relationship between life satisfaction and positive mental health of Vietnamese: A cross-sectional study in COVID-19 pandemic context
Authors: Ngoc Nguyen Be Thi , Huynh Son Van , Nguyen Trong Nguyen , Nguyen-Duong Bao-Tran , Ngo-Thi Thuy-Trinh , Tran-Chi Vinh-Long
Published: November 2022

Vietnam, a middle-income country, has been suffering four waves of the Coronavirus disease 2019 (COVID-19) pandemic and a massive lockdown to suppress the spread of this infectious disease. Consequently, COVID-19 has caused psychological ramifications and affected humankind’s life satisfaction. Because of the lockdown period, numerous people had plentiful time. Hence, they found solace in excessive watching of television and movies, which could lead to post-series depression. The purpose of this study is to investigate the relationship between life satisfaction (LS), post-series depression (PSD), and positive mental health (PMH) and inquire about the mediation effect of satisfaction of life and PSD. A total of 2,572 participants who were voluntarily recruited from various media platforms completed self-report questionnaires, including the Satisfaction with life scale, Post-series depression scale, and Positive Mental Health Scale. This study was assessed using the PLS-SEM approach. The findings of this research discovered (i) a significantly positive effect of LS on PMH; (ii) a significantly negative effect of PSD on PMH; (iii) a significantly negative effect of LS on PSD, and (iv) a significant indirect effect of LS on PMH through PSD. The study provided additional evidence to the relationship between life satisfaction and PMH of individuals. Besides, the negative effects of PSD, which is a non-clinical term for feeling down that frequently appears after individuals finish their much-loved film and TV series, on individuals’ PMH is proved, especially in the COVID-19 pandemic context in which Vietnamese people must remain in their current location.
Spatio-temporal evolution of the COVID-19 across African countries
Authors: Naffeti Bechir , Bourdin Sebastien , Ben Aribi Walid , Kebir Amira , Ben Miled Slimane
Published: November 2022

The aim of this study is to make a comparative study on the reproduction number R0 computed at the beginning of each wave for African countries and to understand the reasons for the disparities between them. The study covers the two first years of the COVID-19 pandemic and for 30 African countries. It links pandemic variables, reproduction number R0, demographic variable, median age of the population, economic variables, GDP and CHE per capita, and climatic variables, mean temperature at the beginning of each waves. The results show that the diffusion of COVID-19 in Africa was heterogeneous even between geographical proximal countries. The difference of the basic reproduction number R0 values is very large between countries and is significantly correlated with economic and climatic variables GDP and temperature and to a less extent with the mean age of the population.
“A passion to change the landscape and drive a renaissance”: The mRNA Hub at Afrigen as decolonial aspiration
Authors: Paremoer Lauren , Pollock Anne
Published: November 2022

In global conversations about COVID-19 vaccine access, Africa has overwhelmingly been characterized as a site of lack. However, the successful reverse engineering of the Moderna vaccine by Afrigen at the mRNA Hub in Cape Town marks a more hopeful path forward. This paper introduces the mRNA Hub and puts it into context of broader decolonial aspirations for African futures in the face of rich countries' disregard. Highlighting ways in which Afrigen's Managing Director's articulations of the endeavor resonate with historical and contemporary calls to dismantle the unequal global order, we argue that the mRNA Hub provides an example of the potential for decolonial solidarity in the post/pandemic period.
Methodological heterogeneity biases physical activity metrics derived from the Actigraph GT3X in multiple sclerosis: A rapid review and comparative study
Authors: Polhemus Ashley , Haag Christina , Sieber Chloé , Sylvester Ramona , Kool Jan , Gonzenbach Roman , von Wyl Viktor
Published: November 2022

Background

Physical activity (PA) is reduced in persons with multiple sclerosis (MS), though it is known to aid in symptom and fatigue management. Methods for measuring PA are diverse and the impact of this heterogeneity on study outcomes is unclear. We aimed to clarify this impact by comparing common methods for deriving PA metrics in MS populations.

Methods

First, a rapid review of existing literature identified methods for calculating PA in studies which used the Actigraph GT3X in populations with MS. We then compared methods in a prospective study on 42 persons with MS [EDSS 4.5 (3.5–6)] during a voluntary course of inpatient neurorehabilitation. Mixed-effects linear regression identified methodological factors which influenced PA measurements. Non-parametric hypothesis tests, correlations, and agreement statistics assessed overall and pairwise differences between methods.

Results

In the rapid review, searches identified 421 unique records. Sixty-nine records representing 51 eligible studies exhibited substantial heterogeneity in methodology and reporting practices. In a subsequent comparative study, multiple methods for deriving six PA metrics (step count, activity counts, total time in PA, sedentary time, time in light PA, time in moderate to vigorous PA), were identified and directly compared. All metrics were sensitive to methodological factors such as the selected preprocessing filter, data source (vertical vs. vector magnitude counts), and cutpoint. Additionally, sedentary time was sensitive to wear time definitions. Pairwise correlation and agreement between methods varied from weak (minimum correlation: 0.15, minimum agreement: 0.03) to perfect (maximum correlation: 1.00, maximum agreement: 1.00). Methodological factors biased both point estimates of PA and correlations between PA and clinical assessments.

Conclusions

Methodological heterogeneity of existing literature is high, and this heterogeneity may confound studies which use the Actigraph GT3X. Step counts were highly sensitive to the filter used to process raw accelerometer data. Sedentary time was particularly sensitive to methodology, and we recommend using total time in PA instead. Several, though not all, methods for deriving light PA and moderate to vigorous PA yielded nearly identical results. PA metrics based on vertical axis counts tended to outperform those based on vector magnitude counts. Additional research is needed to establish the relative validity of existing methods.
Transition of bioeconomy as a key concept for the agriculture and agribusiness development: An extensive review on ASEAN countries
Authors: Wang Tiantian , Yu Zhongkang , Ahmad Riaz , Riaz Samina , Khan Kashif Ullah , Siyal Saeed , Chaudhry Muhammad Asif , Zhang Taiming
Published: November 2022

Transition of bioeconomy plays an important role in the development of any economy. Therefore, the purpose of the current paper is to review the key concept of transition of the bioeconomy in the ASEAN economies that is considered to be an innovative collective method for observing the operation of national economies' numerous sectors that produce and process biological wealth. Based on current literature, it has been reviewed that the transition of bioeconomy is being presented with respect to the theoretical concept, and their essence and bioeconomy size, and the challenges and risks which are related to the bioeconomy transition. The various ways and the area of action along with present strategies which are supporting the development of bio economies and their relationship with the circular economies model had been presented. It was seen in the literature that ASEAN agricultures sectors had been contributing their major part in the development of value-added products and employment, except for Malaysia, Thailand, and Laos. Moreover, the analysis results had also shown that the transition of bioeconomy is considered to be an important concept in the growth of agriculture, forestry, agribusiness, and various sectors that produce and use bio-based raw materials. It was further found that for real bio-economy opportunities, it is essential that there should be a proper national and regional strategy for the bioeconomy development in place and for the development of a proper design and proper management coordination at every level of sectors, enterprises, and provincial systems.
Expression of matrix metalloproteinases (MMPs)−2/-7/-9/-14 and tissue inhibitors of MMPs (TIMPs)−1/-2 in bovine cutaneous fibropapillomas associated with BPV-2 infection
Authors: Daraban Bocaneti Florentina , Altamura Gennaro , Corteggio Annunziata , Tanase Oana Irina , Dascalu Mihaela Anca , Pasca Sorin Aurelian , Hritcu Ozana , Mares Mihai , Borzacchiello Giuseppe
Published: November 2022

Introduction

Bovine papillomaviruses −1/−2 (BPVs) are small non-enveloped double-stranded DNA viruses able to infect the skin of bovids and equids, causing development of neoplastic lesions such as bovine cutaneous fibropapillomas and equine sarcoid. Matrix metalloproteinases (MMPs) are a group of zinc-dependent endopeptidases that degrade basal membrane and extracellular matrix, whose function is essential in physiological processes such as tissue remodeling and wound healing. MMPs activity is finely regulated by a balancing with expression of tissue inhibitors of MMPs (TIMPs), a process that is impaired during tumour development. BPV infection is associated with upregulation of MMPs and /or their unbalancing with TIMPs, contributing to local invasion and impairment of extracellular matrix remodeling in equine sarcoid; however, studies regarding this topic in bovine fibropapillomas are lacking.

Methods

The aim of this study was to perform an immunohistochemical and biochemical analysis on a panel of MMPs and TIMPs in BPV-2 positive bovine cutaneous fibropapillomas vs. normal skin samples.

Results

Immunohistochemistry revealed a cytoplasmic expression of MMP-2 (15/19), a cytoplasmic and perinuclear immunoreactivity of MMP-7 (19/19) and MMP-9 (19/19), along with a cytoplasmic and nuclear pattern of MMP-14 (16/19), accompanied by a cytoplasmic expression of TIMP-1 (14/19) and TIMP-2 (18/19) in tumour samples; western blotting revealed an overexpression of MMP-2 (8/9), MMP-7 (9/9) and MMP-9 (9/9), and a decreased level of MMP-14 (9/9), TIMP-1 (9/9) and TIMP-2 (9/9) in tumour versus normal skin samples. Moreover, gelatine zymography confirmed the expression of pro-active MMP-2 (9/9) and MMP-9 (9/9) and, most importantly, indicated the presence and increased activity of their active forms (82 and 62 kDa, respectively) in tumour samples.

Discussion

This is the first study describing MMPs and TIMPs in bovine cutaneous fibropapillomas and our results suggest that their unbalanced expression in presence of BPV-2 may play a significant role in tumour development. A further analysis of supplementary MMPs and TIMPs could bring new important insights into the papillomavirus induced tumours.
Measuring the Image of Private University as a Generic Product: Validation of a Scale
Authors: Alcaide-Pulido Purificación , Gutiérrez-Villar Belén , Carbonero-Ruz Mariano
Published: November 2022

The compulsory nature of online training in university education, brought about by COVID-19, has opened the door to the emergence of several potential competitors in the university space. Thus, measuring a university’s image may have even greater importance for the management and differentiation of universities in the new post-COVID-19 horizon. This study aims to test whether a standardized scale of brand image measurement is valid for measuring the image of the “private university” product. A non-probabilistic convenience sample was chosen, collecting information from 728 citizens from the same territory (Andalusia). The procedure to validate the scale involves dividing the sample (728) into two sub-sets: one to establish the scale, and the other to validate the results. The methodology applied is Confirmatory Factor Analysis using EQS 6.3 software. The scale was validated, and the main results show that people favor the quality of private universities, their commitment to society, and the perfect option that they are. Additionally, results show the idea that private universities present characteristics absent from public ones as non-significant, and do not agree that private universities provide a high value concerning the price that has to be paid.
Sliding Mode Backstepping Control for the Ascent Phase of Near-Space Hypersonic Vehicle Based on a Novel Triple Power Reaching Law
Authors: Huang Shutong , Jiang Ju , Li Ouxun
Published: November 2022

This paper presents a novel sliding mode backstepping control scheme for the ascent phase of a near-space hypersonic vehicle (NSHV) base on a triple power reaching law (TPRL). A new model transformation is proposed for NSHV with uncertain parameters subject to uncertainties during ascent phase. To shorten the reaching time and reduce the chattering in sliding mode scheme, TPRL is proposed. Then, based on TPRL, a sliding mode backstepping control scheme is proposed, which is combined with new adaptive laws to further reduce the adverse impact of uncertainties. Simulation results demonstrate that TPRL is effective, and the proposed controller for the ascent phase of NSHV is robust with respect to uncertainties.
Precision Feedback Control Design of Miniature Microwave Discharge Ion Thruster for Space Gravitational Wave Detection
Authors: Zhou Junjie , Pang Aiping , Liu Hui , Zhou Hongbo , Niu Xiang
Published: November 2022

The space gravitational wave detection mission has put forward multiple performance requirements for micro-newton thrusters, such as “fast response, high resolution, and low noise”. In order to solve the problem that the traditional open-loop propulsion system can hardly meet the high-precision performance, the microwave ion thruster with high accuracy and controllability of thrust estimation is adopted as the research object, and the precision feedback control method combining analog circuit and digital control technology is adopted to achieve a resolution of less than 0.1 μN, fast response of 20 ms, and 10−3~1 Hz frequency band noise less than 0.05 μN/Hz1/2 of the thrust performance. Experimental results show that, compared with open-loop regulation, the feedback control can effectively suppress various noises and disturbances caused by device temperature drift and complex operating characteristics of the thruster, and the anti-aliasing filter scheme adopted in this paper can suppress the folding of high-frequency data in the low- and medium-frequency bands during the digital control process, while it can effectively improve the system performance and reduce the impact of thrust noise. The feedback control strategy proposed in this paper verifies the possibility of a microwave ion thruster as an actuator for super “static and precise” space experiment satellites and provides a feasible solution for the application of a microwave ion thruster in deep space science experiment missions such as gravitational wave detection.
Potassium Application Increases Cotton (Gossypium hirsutum L.) Fiber Length by Improving K+/Na+ Homeostasis and Potassium Transport Capacity in the Boll-Leaf System under Moderate Salinity
Authors: Zhu Junjun , Sun Liyuan , Ju Feiyan , Wang Zhuo , Xiong Cai , Yu Huilian , Yu Kai , Huo Yuyang , Khattak Wajid Ali , Hu Wei , Wang Shanshan , Zhou Zhiguo , Chen Binglin
Published: November 2022

Cotton has a high salt tolerance. However, due to the high salt content and low K+/Na+ ratio in saline soils, cotton yield and fiber quality are difficult to improve. To investigate the effects of potassium (K) on cotton fiber length under salt stress, a two-year bucket-based field experiment was conducted using two different cultivars (CCRI 79, salt tolerant, and Simian 3, salt sensitive). Three K rates (K0, 0 kg K2O ha−1; K150, 150 kg K2O ha−1; and K300, 300 kg K2O ha−1) were applied at low, middle, and high soil electrical conductivities (S1, 1.7–1.8 dS m−1; S2, 6.4–6.9 dS m−1; and S3, 10.6–11.8 dS m−1) to investigate the absorption, transport, and distribution characteristics of K+ and Na+ in the boll-leaf system (including the leaf subtending the cotton boll (LSCB), fruiting branch, boll shell, and fiber) of both cotton cultivars, as well as the relationship with fiber length. The results showed that K application (K150 and K300) significantly increased the cotton fiber length under salt stress, with the largest fiber length alleviation coefficients (AC) in the middle fruiting branches. The AC decreased with an increase in salt stress and was greater in CCRI 79 than in Simian 3. The K150 treatment (soil K+/Na+ = 1/13) completely mitigated the reduction in fiber length caused by S2 salt stress in CCRI 79, whereas the K300 treatment (soil K+/Na+ = 1/10) completely eased the reduction in fiber length caused by S2 salt stress in Simian 3. An application of K under salt stress increased the K+ content and K+/Na+ ratio in the soil and the organs of the boll-leaf system, regulated the K+/Na+ homeostasis in the boll-leaf system, enhanced the K+-selective transport coefficient (SK-Na) in the LSCB, maintained a high K+/Na+ ratio in the fiber, and mitigated the fiber length reduction. In conclusion, the fiber length reduction in salt-tolerant cultivars was completely mitigated by K150 (i.e., soil K+/Na+ = 1/13) under moderate salt stress; however, it was not completely mitigated by K application under high salt stress.
Optimizing Phosphorus Application for Winter Wheat Production in the Coastal Saline Area
Authors: Liu Lu , Miao Qi , Wang Hongye , Xue Yanfang , Qi Shijun , Zhang Jishi , Li Junchao , Meng Qingfeng , Cui Zhenling
Published: November 2022

Phosphorous (P) fixation in saline soils is a concern worldwide. To investigate optimization strategies for P fertilizer application that improve P use efficiency (PUE) and crop yield in saline soil, in terms of P sources and rates, we conducted a two year field experiment in the coastal saline area of China to investigate the effects of P rates and sources, including superphosphate (SSP), monoammonium phosphate (MAP) and ammonium polyphosphate (APP) on yield, aboveground P uptake, agronomy efficiency (AEP), and soil available P of winter wheat (Triticum aestivum L.). Wheat yield, under the three P sources, increased with P rates and reached a plateau under 20 kg P ha−1 SSP,18 kg P ha−1 MAP, and 17 kg P ha−1 MAP, respectively. The application of SSP increased the wheat yield by 9–11% compared to MAP and APP. The aboveground P uptake of winter wheat under SSP was 14% and 13% higher than MAP and APP, respectively, under the optimal P application rate. The AEP under SSP was higher than the other two P sources under the same P rate. SSP increased the soil Ca2+ concentration by 20–42%, but decreased the Na+ concentration by 14–18% at the P rate of 26 kg P ha−1 in all soil layers, including 0–20, 20–40, 40–60 cm, compared to CK (0 kg P ha−1). The soil Olsen-P concentration under APP was higher than the other P sources. This study suggests that optimizing P sources and rates can improve wheat yield, PUE, and soil quality in the coastal saline soil.
Complete Chloroplast Genome Sequence of a New Variety of Brasenia schreberi: Genome Characteristics, Comparative Analysis, and Phylogenetic Relationships
Authors: Sun Yue , Li Mengyao , Ma Junying , He Maolin , Zheng Yangxia
Published: November 2022

This study sequenced and assembled the chloroplast (cp) genome of Brasenia schreberi cv. ‘Mahu Chuncai’, a novel variety of B. schreberi rich in nutrients with distinctive characteristics, unlike other varieties in China. The cpDNA genome of ‘Mahu Chuncai’ has a typical quadripartite structure, with a full length of 158,973 bp, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The phylogenetic analysis revealed that all species can be divided into three main clades. Results from inverted repeats (IR) boundary analysis revealed substantial differences between Brasenia and Cabomba species. The cpDNA genome of B. schreberi identified was strongly related to Brasenia species but appeared to be a distant relative of Cabomba aquatica more than other species in Cabombaceae. In contrast with the species from Cabombaceae, ‘Mahu Chuncai’ was a close relative of B. schreberi MN315507.1, which was a distant relative of C. aquatica MG720559.1. Furthermore, we found four potential molecular markers, i.e., ycf1 in the IR region, psbT in the LSC region, and ndhF and rps15 in the SSC region. Collectively, our findings confirm the phylogenetic evolution and cultivation origin of B. schreberi. We identified genetic characteristics and nucleotide diversity hotspots, which provides a theoretical basis for additional research on variety identification, germplasm resources, and molecular breeding of the precious vegetable.
Greenhouse Gas Emissions with Low Disturbance Liquid Dairy Manure Incorporation into a Live Winter Cereal Cover Crop-Corn System
Authors: Sherman Jessica , Young Eric
Published: November 2022

Dairy manure is an important nutrient source for crops but can also contribute to ammonia (NH3) and greenhouse gas (GHG) emissions. While incorporating manure into the soil reduces nutrient loss potential in surface runoff, impacts on GHGs are unclear. Here, our objective was to quantify NH3, nitrous oxide (N2O), methane (CH4), and carbon dioxide (CO2) fluxes for two seasons after liquid dairy manure was spring-applied to a live winter cereal cover crop-corn system with different incorporation methods. Broadcast application and no manure controls were compared to manure incorporated by vertical tillage (VT) or chisel plowing (CP). Corn yields did not differ in 2018 but were greater for CP in 2019. Mean NH3 emissions for VT were 70 and 23% of broadcast and 7 and 11% of broadcast for CP in 2018 and 2019, respectively. While VT N2O-N fluxes were also about 70% lower than broadcast both years, CO2 fluxes were larger for VT. On average, CP and VT had 16 and 4% lower global warming potential (GWP) index values than broadcast, respectively. Despite differing effects on N2O, our results showed that CP more effectively conserved NH3 while reducing GWP from liquid manure compared to VT, stressing the importance of site-specific soil-manure-tillage interactions when quantifying dairy system GHG fluxes.
Leaf Color Chart (LCC)-Based Precision Nitrogen Management for Assessing Phenology, Agrometeorological Indices and Sustainable Yield of Hybrid Maize Genotypes under Temperate Climate
Authors: Fayaz Suhail , Kanth Raihana Habib , Bhat Tauseef Ahmad , Valipour Mohammad , Iqbal Rashid , Munir Awais , Nazir Aijaz , Mir Mohd Salim , Ahanger Shafat Ahmad , Al-Ashkar Ibrahim , Sabagh Ayman El
Published: November 2022

Excessive nitrogenous fertilization in years resulted in larger nitrogen and profit losses. This problem can be reduced by using need-based and time-specific nitrogen management. Therefore, a field experiment was carried out during the Kharif season of 2019 and 2020 in order to evaluate the impact of precision nitrogen management on the phenology, yield and agrometeorological indices of hybrid maize genotypes at the Agronomy Research Farm, FoA Wadura, Sopore, SKUAST-Kashmir. The experiment was carried out in split-plot design consisting of maize hybrids (Shalimar Maize Hybrid-2 Vivek-45 and Kanchan-517) as main plot treatments and precision nitrogen management (T1: Control, T2: Recommended N, T3: 25% N as basal ≤ LCC 3@20 kg N ha−1, T4: 25% N as basal ≤ LCC 3@30 kg N ha−1, T5: 25% N as basal ≤ LCC 4@20 kg N ha−1, T6: 25% N as basal ≤ LCC 4@30 kg N ha−1, T7: 25% N as basal ≤ LCC 5@20 kg N ha−1 and T8: 25% N as basal ≤ LCC 5@30 kg N ha−1) as sub-plot treatments. Results demonstrated that maize hybrids showed a non-significant difference in attaining different phenophases during both years. However, Shalimar Maize Hybrid-2 demonstrated higher grain (62.35 and 60.65 q ha−1) and biological yield (170.26 and 165.86 q ha−1), a higher number of days to attain different phenological stages in comparison to Vivek-45 and Kanchan-517 thereby achieved higher heat units, PTUs, HTUs, PTI. The application of nitrogen through LCC ≤ 5@30 kg N ha−1 noted higher grain yield (61.27 and 59.13 q ha−1) and biological yield (171.30 and 166.13 q ha−1) during 2019 and 2020 respectively. Higher values of Growing degree days (GDD), Heliothermal units (HTU), Photothermal units (PTU), Phenothermal index (PTI), heat use efficiency (HUE) and radiation use efficiency (RUE) were observed in the application of nitrogen through LCC ≤ 5@30 kg N ha−1 and required the highest number of days to reach different phenophases than other treatments during crop growing seasons of 2019 and 2020. The results demonstrated that Nitrogen application based on LCC ≤ 5@30 proved effective and should be adopted in maize hybrids especially in Shalimar Maize Hybrid-2 to attain higher yield under the temperate climate of Kashmir Valley.
Identification of Key Regulatory Factors of Molecular Marker TGS377 on Chromosome 1 and Its Response to Cold Stress in Tomato
Authors: Zhang Jia-Qi , Tao Jian-Ping , Song Liu-Xia , Zhang Rong-Rong , Liu Hui , Zhao Tong-Min , Zhu Wei-Min , Xiong Ai-Sheng
Published: November 2022

Low temperature, as a kind of stress factor, often leads to tomato growth stagnation or yield reduction or even no harvest in production. At present, numerous genes have been shown to be involved in the regulation of cold resistance in the tomato. Complex regulatory mechanisms responding to low temperature stress in the tomato are still unclear in their details. In this study, six accessions of tomato (‘NL-7’, ‘NL-15’, ‘NL-18’, ‘NL-21, ‘NL-37’, and ‘NL-67’) with different cold tolerance were selected to detect the response to low temperature. The results showed that ‘NL-15’, ‘NL-18’, and ‘NL-21’ tomato accessions had cold tolerance under 8 °C/6 °C (day/night) for 15-day treatments. The TGS377 molecular marker, closely related to cold tolerance, was located on chromosome 1. The potential factors were identified and bioinformatics analysis within 50 kb upstream and downstream of TGS377. Fifteen genes were identified, and their structural analysis and functional annotation were also performed. The expression levels of Solyc01g008480 and Solyc01g150104 in the cold-sensitive tomato accessions (‘NL-7’, ‘NL-37’, and ‘NL-67’) were higher than that in the cold-tolerant accessions (‘NL-15’, ‘NL-18’ and ‘NL-21’). The expression levels of Solyc01g008390 and Solyc01g008410 in the cold-tolerant tomato ‘NL-18’ accession was significantly higher than that in the cold-sensitive accessions (‘NL-15’, ‘NL-18’, and ‘NL-21’). The results suggested that these genes may be involved in regulating low temperature response in the tomato, which lays a foundation for the search of potential cold response regulators in the tomato.
Diagnosing Intramammary Infection: Meta-Analysis and Mapping Review on Frequency and Udder Health Relevance of Microorganism Species Isolated from Bovine Milk Samples
Authors: Kurban Daryna , Roy Jean-Philippe , Kabera Fidèle , Fréchette Annie , Um Maryse Michèle , Albaaj Ahmad , Rowe Sam , Godden Sandra , Adkins Pamela R. F. , Middleton John R. , Gauthier Marie-Lou , Keefe Greg P. , DeVries Trevor J. , Kelton David F. , Moroni Paolo , Veiga dos Santos Marcos , Barkema Herman W. , Dufour Simon
Published: November 2022

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry provides accurate species-level identification of many, microorganisms retrieved from bovine milk samples. However, not all those microorganisms are pathogenic. Our study aimed to: (1) determine the species-specific prevalence of microorganisms identified in bovine milk of apparently healthy lactating quarters vs. quarters with clinical mastitis (CM); and (2) map current information and knowledge gaps on udder health relevance of microorganisms retrieved from bovine milk samples. A mixed study design (meta-analysis and mapping review) was chosen. We gathered several large Canadian, US and Brazilian data sets of MALDI-TOF results for organisms cultured from quarter milk samples. For meta-analysis, two datasets (apparently healthy quarters vs. CM samples) were organized. A series of meta-analyses was conducted to determine microorganisms’ prevalence. Then, each species reported was searched through PubMed to investigate whether inflammation (increased somatic cell count (SCC) or signs of CM) was associated with microorganism’s recovery from milk. A total of 294 different species of microorganisms recovered from milk samples were identified. Among 50,429 quarter-milk samples from apparently healthy quarters, the 5 most frequent species were Staphylococcus chromogenes (6.7%, 95% CI 4.5–9.2%), Aerococcus viridans (1.6%, 95% CI 0.4–3.5%), Staphylococcus aureus (1.5%, 95% CI 0.5–2.8%), Staphylococcus haemolyticus (0.9%, 95% CI 0.4–1.5%), and Staphylococcus epidermidis (0.7%, 95% CI 0.2–1.6%). Among the 43,924 quarter-milk CM samples, the 5 most frequent species were Escherichia coli (11%, 95% CI 8.1–14.3%), Streptococcus uberis (8.5%, 95% CI 5.3–12.2%), Streptococcus dysgalactiae (7.8%, 95% CI 4.9–11.5%), Staphylococcus aureus (7.8%, 95% CI 4.4–11.9%), and Klebsiella pneumoniae (5.6%, 95% CI 3.4–8.2%). When conducting the PubMed literature search, there were 206 species identified by MALDI-TOF for which we were not able to find any information regarding their association with CM or SCC. Some of them, however, were frequently isolated in our multi-country dataset from the milk of quarters with CM (e.g., Citrobacter koseri, Enterococcus saccharolyticus, Streptococcus gallolyticus). Our study provides guidance to veterinarians for interpretation of milk bacteriology results obtained using MALDI-TOF and identifies knowledge gaps for future research.
Longitudinal Analysis of Variability in Fecal Glucocorticoid Metabolite Concentrations in Three Orangutans (Pongo pygmaeus pygmaeus and Pongo pygmaeus abelii) before, during, and after Transition from a Regular Habitat Environment to Temporary Housing in Indoor Holding Facilities
Authors: Fink Laurel B. , Mukobi Asaba , Gruber Lindsey , Reed Colleen , DeLibero Jason , Jackson Scott , Neill Sierra , Walz Julia , Sines Cydney , VanBeek Becca , Scarlata Candace D. , Wielebnowski Nadja
Published: November 2022

Considerable research has been conducted on the effects of inter-institutional transfers, but far less consideration has been given to intra-institutional transfers and extended housing in off-habitat holding. On 15 May 2018, The Oregon Zoo’s orangutans (n = 3) were moved from the Red Ape Reserve (RAR) to the Veterinary Medical Center (VMC) indoor holding areas and remained there until 22 December 2020, resulting in over two years of housing in a facility not specifically designed for orangutans. This study aimed to quantify potential changes in fecal glucocorticoid metabolites (fGM) typically associated with increased adrenal activity as a result of transfers, as well as potential differences in fGM concentrations associated with housing in the two different types of locations. We collected fecal samples from all orangutans during three time periods: the initial housing at RAR (RAR1), the time spent at VMC holding (VMC), and the return to RAR (RAR2). Samples were analyzed using enzyme-immunoassay (EIA) analyses and compared using two-way ANOVA tests with Games–Howell post-hoc evaluations. The results of our analyses showed the following: (1) significant differences in fGM concentrations based on location in two orangutans, with the highest fGM concentration occurring in fecal samples collected at the VMC; and (2) a lack of significant fGM peaks following multiple intra-institutional transfers for all three orangutans. Though requiring further corroboration through future studies, we speculated that pre-transfer behavior training and intensive, continued care by familiar animal care staff may have helped to mitigate the stress responses commonly associated with transfers and major changes in housing. Furthermore, this study highlights the individualistic nature of the stress response, as illustrated by the substantial variation in fGM concentrations across different housing regimens in the three orangutans.
Positive Airway Cultures in Dogs and Cats Receiving Mechanical Ventilation for Tick Paralysis
Authors: Tso Suzanne Suk Kwan , Leister Ellie , Sharp Claire Rebecca , Heller Jane , Gibson Justine S.
Published: November 2022

Animals with tick paralysis often require mechanical ventilation (MV) but previous publications have identified knowledge gaps regarding the development of bacterial pneumonia, and the specific pathogens involved. The objectives of this study were to describe the clinical course and culture and susceptibility profiles of bacteria isolated from airway samples of dogs and cats mechanically ventilated for tick paralysis that had positive airway cultures. Medical records were reviewed, and cases included if they had a positive airway sample culture during MV for tick paralysis. Twenty-four dogs and two cats were included. Most (85%) received empirical antimicrobials before airway sampling. The most common organisms isolated included Staphylococcus spp. (11), Klebsiella spp. (9), Enterococcus faecalis (8), Escherichia coli (6), Enterococcus faecium (3), Pseudomonas aeruginosa (4), and Mycoplasma spp. (3). Evidence of aspiration pneumonia was present in 22/25 (88%) cases that had thoracic radiographs performed. Seventy-seven percent of cases received antimicrobials to which the cultured bacteria were susceptible during hospitalisation. The median duration of MV was 4 days (range 1–10). Most (77%) survived to discharge, 19% were euthanised, and one died. In a multivariable logistic regression analysis it was identified that selection of antimicrobials to which the causative bacteria are susceptible was associated with survival to discharge (Odds ratio 45.8, p = 0.014; 95%CI 1.98–14,808), as was length of MV, with every day an animal is ventilated associated with a 4.7 times increased chance of survival (p = 0.015; 95% CI 1.21–78.4).
The Effect of the Inclusion of Different Concentrates in Feed Rations on the Contents of Tocopherols, β-Carotene and Retinol in the Livers and Longissimus dorsi Muscles of Farm-Raised Fallow Deer (Dama dama L.)
Authors: Czurgiel Sylwia , Antoszkiewicz Zofia , Mazur-Kuśnirek Magdalena , Bogdaszewski Marek
Published: November 2022

This study analyzed the chemical composition and contents of β-carotene, tocopherols and retinol in the longissimus dorsi (LD) muscles and livers of fallow deer (Dama dama L.) fed diets based on hay containing different concentrates (hay and oats—HO; hay, oats and protein concentrate—HOP; hay and pellets—HP). The provitamin and lipophilic vitamin contents in the samples of feed and animal tissues were determined by high-performance liquid chromatography (HPLC). The contents of retinol were nearly two-fold higher (p < 0.002) in the livers and many-fold higher in the LD muscles of fallow deer fed the HOP and HP diets. β-carotene was not identified in the livers or LD muscles of fallow deer. The concentrations of α-tocopherol and total tocopherols were higher in the livers and LD muscles of the animals that received the HO and HP diets, but the noted differences were not significant. The inclusion of various concentrates in the feed rations had no influence on the proximate chemical compositions of the livers or LD muscles of fallow deer.
First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques
Authors: Idris Sanaa M. , Ali Eva A. , Elmagzoub Wisal A. , Okuni Julius B. , Mukhtar Mohamed E. , Ojok Lonzy , Eltayeb ElSagad , Abd El Wahed Ahmed , Eltom Kamal H. , Gameel Ahmed A.
Published: November 2022

Paratuberculosis (PTB) has been reported in the Sudan in cattle and goats for more than 50 years but has never been reported in sheep. However, suspicion of the disease in a breeding flock of sheep in Khartoum North locality was made due to a history of unknown cause of loss of weight. Blood and faecal samples were collected from all animals (N = 59): harvested sera were tested for anti-Mycobacterium avium subsp. paratuberculosis (MAP) antibodies by Enzyme Linked Immunosorbent Assay (ELISA); faeces were screened for acid-fast bacilli by Ziehl–Neelsen staining, tested for MAP DNA by recombinase polymerase amplification (RPA) and some faecal samples were cultured for MAP isolation. Typical MAP acid-fast bacilli were seen in 10.2% (6/59) of the faecal smears, 37.5% of the tested faecal samples (12/32) were positive for MAP DNA and only 3 (5.1%) animals were seropositive for MAP. MAP positive cultures were obtained from 2 out the 6 samples showing typical MAP acid-fast bacilli; the isolates were confirmed by real-time PCR and sequencing. As sheep are animals of utmost economic importance as the main export animals for the country, this first report of ovine PTB warrants special considerations and more investigations for planning control programmes of the disease.
The Effect of Anthocyanins from Dioscorea alata L. on Antioxidant Properties of Perinatal Hainan Black Goats and Its Possible Mechanism in the Mammary Gland
Authors: Zhang Yuanxin , Shi Huiyu , Yun Yanhong , Feng Haibo , Wang Xuemei
Published: November 2022

(1) Background: The mammary glands of the perinatal goats are susceptible to reactive oxygen species (ROS) leading to oxidative injury. Although Dioscorea alata L. is rich in anthocyanins with high safety and excellent free-radical-scavenging ability, the effect and mechanism of Dioscorea alata L. anthocyanins (DAC) on the antioxidant capacity of the black Hainan goat has been the subject of few studies to date; (2) Methods: For this reason, feeding experiments were performed by feeding experimental diets, and the pre-protective capacity of DAC on goat mammary epithelial cells was explored on the basis of the established model of H2O2 injury; (3) Results: As well as altering rumen fermentation parameters in perinatal female goats, dietary challenge also improves antioxidant capacity in their blood and milk. thereby enhancing children’s antioxidant capacity and increasing their resistance to oxidative stress. However, we also found that DAC pretreatment was capable of activating both Nrf2 and MAPK/JNK pathways, which results in enhanced antioxidase activity and elimination of ROS; (4) Conclusions: Together, these findings suggest that DAC may have a pre-protective role on perinatal Hainan black goats through the regulation of Nrf2 and MAPK/JNK pathways in GMEC.