bluuebunny/crossref_metadata_2025 huggingface dataset:
Underwater Noise Monitoring with Real-Time and Low-Cost Systems, (The CORMA Experience)
Authors: Diviacco Paolo , Nadali Antonio , Iurcev Massimiliano , Burca Mihai , Carbajales Rodrigo , Gangale Matteo , Busato Alessandro , Brunetti Fabio , Grio Lorenzo , Viola Alberto , Potleca Nikolas
Published: April 2021

Marine life can be severely affected by anthropogenic underwater noise. This latter increased proportionally to the rise of human activities such as maritime traffic, marine civil engineering works, oil- and gas-related activities or offshore wind farms; so much so that, currently, it can be considered a threat to the environment. Assessing underwater noise requires quite some investments both in personnel and instrumentation. If this is affordable by several governmental and scientific organizations, this cannot be extended straightforwardly to all research initiatives or to developing countries. In addition, time and geographic coverage of monitoring can also be significantly limited by the costs of multiple installations. We explore the possibility to use a solution based on off-the-shelf and low-cost technologies combined with a scalable infrastructure developed with open-source tools only. The perspective to avoid proprietary solutions allows great flexibility in extending the current paradigm toward real-time transmission, processing, and web-based data access. Our solution has been deployed at sea in November 2020 and is providing data continuously ever since. First results from the analysis of these data allowed us to highlight several interesting abiotic and anthropogenic temporal patterns.
Invasive Species Control: Predation on the Alien Crab Percnon gibbesi (H. Milne Edwards, 1853) (Malacostraca: Percnidae) by the Rock Goby, Gobius paganellus Linnaeus, 1758 (Actinopterygii: Gobiidae)
Authors: Tiralongo Francesco , Messina Giuseppina , Lombardo Bianca Maria
Published: April 2021

Invasive alien species (IAS) are one of the greatest causes of native species extinction. Indeed, they represent a global threat for biodiversity and can also affect the economy and human health. The colonization success of IAS is presumably not only due to their biological and ecological characteristics, but also to the lack of predators and/or parasites in the invaded new areas. In the present work, we demonstrate evidence of predation of the invasive alien crab Percnon gibbesi (H. Milne Edwards, 1853) by the Rock Goby Gobius paganellus Linnaeus, 1758. The diet of G. paganellus was studied analyzing the stomach content of 162 specimens collected in the central Mediterranean Sea. The results obtained from the calculation of the diet indices, namely, frequency of occurrence (%F), percentage weight (%W), percentage abundance (%N), and the Index of Relative Importance (%IRI), showed that small benthic crustaceans were the main prey types. Additionally, these indices and the Levins’ index (Bi) clearly indicated that the invasive crab P. gibbesi was by far the most abundant prey type in the diet of G. paganellus. The relevance of this predator–prey interaction and the role of native species for the biological control of invasive ones are discussed. We also provide a general view on the diet of G. paganellus and other biological and ecological aspects of specimens studied from the central Mediterranean Sea.
Modeling Riverbank Slope Reinforcement Using Anti-Slide Piles with Geocells
Authors: Liu Zhen , Liu Pengzhen , Zhou Cuiying , Li Yasheng , Zhang Lihai
Published: April 2021

Geocells are increasingly used in engineering applications, but the design of riverbank slope reinforcements that use only geocells limits reinforcement performance. Moreover, the design and use of anti-slide piles with geocells are mainly based on experiences that are unsupported by theoretical models. In this paper, by combining the confinement effect and vertical action mechanism of geocells, the horizontal friction mechanism of the geocell layer and the vertical support mechanism of piles, a theoretical model of riverbank slope reinforced by anti-slide piles with geocells was constructed. In addition, to describe the mechanical behavior of a riverbank slope reinforced by anti-slide piles with geocells, the slip-resisting mechanism of the anti-slide pile with interaction between geocells and their internal filler is considered in the model. Furthermore, to investigate the influence of changes in water level on riverbank slope stability, the developed model takes into account settlement, lateral displacement, pile bending moment and pile axial force. The model predications were validated by the field measurement data. The results from a series of parametric studies show that the use of anti-slide pile and geocells can effectively reduce the settlement and the lateral displacement of a riverbank slope. The developed model could contribute to an optimal design of anti-slide pile with geocells for enhancing the stability of a riverbank slope.
Production–Living–Ecological Conflict Identification Using a Multiscale Integration Model Based on Spatial Suitability Analysis and Sustainable Development Evaluation: A Case Study of Ningbo, China
Authors: Lin Gang , Fu Jingying , Jiang Dong
Published: April 2021

Production–living–ecological space (PLES) basically covers the scope of spatial activities in people’s material production and spiritual life and is the basic carrier of human social development and economic activities. The coordinated development of PLES is an effective method to mitigate land-use conflicts to achieve balanced and coordinated development of the region. However, so far, compared with the single-scale study based on administrative unit, the PLES conflicts between microcosmic grid-scale receives less attention. Considering the important scale problems of the geographical study, this study aims to analyze the synergetic degree of PLES under different scales (administrative-unit, grid, and integrated multiscale) and to scientifically diagnose land use conflicts in Ningbo, China. Results indicated that production land and ecological land in Ningbo were continuously occupied by human activities from 2010 to 2018. The lowest and lower suitability areas of ecological space in Ningbo increased from 2010 to 2018. Land ecological suitability was seriously affected by urban expansion, its ecological value was reduced, and the PLES developed towards the trend of being uncoordinated. Multiscale coupling analysis showed that the PLES in Ningbo was in less conflict on the whole, but with the development of the economy, the coupling coordination degree of PLES was also damaged. This study establishes the different scales of a PLES coupling coordination development degree evaluation index system and enriches the methods of multiscale land use fusion conflict diagnosis and also provides a scientific reference for the optimized and sustainable development of regional territorial space.
Silane-Coating Strategy for Titanium Functionalization Does Not Impair Osteogenesis In Vivo
Authors: Senna Plinio Mendes , de Almeida Barros Mourão Carlos Fernando , Mello-Machado Rafael Coutinho , Javid Kayvon , Montemezzi Pietro , Del Bel Cury Altair Antoninha , Meirelles Luiz
Published: April 2021

Silane-coating strategy has been used to bind biological compounds to the titanium surface, thereby making implant devices biologically active. However, it has not been determined if the presence of the silane coating itself is biocompatible to osseointegration. The aim of the present study was to evaluate if silane-coating affects bone formation on titanium using a rabbit model. For this, titanium screw implants (3.75 by 6 mm) were hydroxylated in a solution of H2SO4/30% H2O2 for 4 h before silane-coating with 3-aminopropyltriethoxysilane (APTES). A parallel set of titanium screws underwent only the hydroxylation process to present similar acid-etched topography as a control. The presence of the silane on the surface was checked by x-ray photoelectron spectroscopy (XPS), with scanning electron microscopy (SEM) and atomic force microscopy (AFM). A total of 40 titanium screws were implanted in the tibia of ten New Zealand rabbits in order to evaluate bone-to-implant contact (BIC) after 3 weeks and 6 weeks of healing. Silane-coated surface presented higher nitrogen content in the XPS analysis, while micro- and nano-topography of the surface remained unaffected. No difference between the groups was observed after 3 and 6 weeks of healing (p > 0.05, independent t-test), although an increase in BIC occurred over time. These results indicate that silanization of a titanium surface with APTES did not impair the bone formation, indicating that this can be a reliable tool to anchor osteogenic molecules on the surface of implant devices.
Enhanced Structural Stability and Electrochemical Performance of LiNi0.6Co0.2Mn0.2O2 Cathode Materials by Ga Doping
Authors: Liu Zhibei , Li Jiangang , Zhu Meijie , Wang Li , Kang Yuqiong , Dang Zhaohan , Yan Jiasen , He Xiangming
Published: April 2021

Structural instability during cycling is an important factor affecting the electrochemical performance of nickel-rich ternary cathode materials for Li-ion batteries. In this work, enhanced structural stability and electrochemical performance of LiNi0.6Co0.2Mn0.2O2 cathode materials are achieved by Ga doping. Compared with the pristine electrode, Li[Ni0.6Co0.2Mn0.2]0.98Ga0.02O2 electrode exhibits remarkably improved electrochemical performance and thermal safety. At 0.5C rate, the discharge capacity increases from 169.3 mAh g−1 to 177 mAh g−1, and the capacity retention also rises from 82.8% to 89.8% after 50 cycles. In the charged state of 4.3 V, its exothermic temperature increases from 245.13 °C to more than 271.24 °C, and the total exothermic heat decreases from 561.7 to 225.6 J·g−1. Both AC impedance spectroscopy and in situ XRD analysis confirmed that Ga doping can improve the stability of the electrode/electrolyte interface structure and bulk structure during cycling, which helps to improve the electrochemical performance of LiNi0.6Co0.2Mn0.2O2 cathode material.
A Strategy for Dimensionality Reduction and Data Analysis Applied to Microstructure–Property Relationships of Nanoporous Metals
Authors: Huber Norbert
Published: April 2021

Nanoporous metals, with their complex microstructure, represent an ideal candidate for the development of methods that combine physics, data, and machine learning. The preparation of nanporous metals via dealloying allows for tuning of the microstructure and macroscopic mechanical properties within a large design space, dependent on the chosen dealloying conditions. Specifically, it is possible to define the solid fraction, ligament size, and connectivity density within a large range. These microstructural parameters have a large impact on the macroscopic mechanical behavior. This makes this class of materials an ideal science case for the development of strategies for dimensionality reduction, supporting the analysis and visualization of the underlying structure–property relationships. Efficient finite element beam modeling techniques were used to generate ~200 data sets for macroscopic compression and nanoindentation of open pore nanofoams. A strategy consisting of dimensional analysis, principal component analysis, and machine learning allowed for data mining of the microstructure–property relationships. It turned out that the scaling law of the work hardening rate has the same exponent as the Young’s modulus. Simple linear relationships are derived for the normalized work hardening rate and hardness. The hardness to yield stress ratio is not limited to 1, as commonly assumed for foams, but spreads over a large range of values from 0.5 to 3.
Evaluation of Cortical Bone Microdamage and Primary Stability of Orthodontic Miniscrew Using a Human Bone Analogue
Authors: Teekavanich Chutimont , Uezono Masayoshi , Takakuda Kazuo , Ogasawara Takeshi , Techalertpaisarn Paiboon , Moriyama Keiji
Published: April 2021

Orthodontic miniscrews have gained popularity; however, they have some drawbacks, including screw loosening that results from bone resorption caused by excess microdamage created during screw insertion. Pilot hole preparation through the cortical bone is considered beneficial to avoid such microdamage, while an overly large pilot hole impairs primary stability. Hence, we used a human bone analogue to evaluate the microdamage and primary stability to estimate the optimal pilot hole size that would minimize the screw loosening risk. Ti6Al4V orthodontic miniscrews and 1.0-mm-thick synthetic cortical bone pieces were prepared. Various compressive loads were applied in indentation tests to test pieces’ surfaces, and the microdamaged areas were confirmed as stress-whitening zones. Screw insertion tests were performed in which a miniscrew was inserted into the test pieces’ pilot hole with a diameter of 0.7–1.2 mm in 0.1-mm intervals, and the stress-whitening area was measured. The insertion and removal torque were also measured to evaluate primary stability. The stress-whitening areas of the 1.0–1.2 mm pilot hole diameter groups were significantly smaller than those of the other groups (p < 0.05), whereas the 0.9 and 1.0 mm pilot hole diameter groups showed higher primary stability than other groups. In conclusion, the bone analogue could be utilized to evaluate microdamage in cortical bones and the primary stability of miniscrews.
Histological and Histomorphometric Comparison of Innovative Dental Implants Laser Obtained: Animal Pilot Study
Authors: Filiberto Mastrangelo , Daniele Botticelli , Franco Bengazi , Antonio Scarano , Adriano Piattelli , Giovanna Iezzi , Raimondo Quaresima
Published: April 2021

Objective: Evaluation of the in vivo bone response of two innovative titanium surfaces ytterbium laser active fiber obtained (L1-L2) compared to a sandblasted and acid etched (SBAE) during early phase of osseointegration. Material and Methods: Three implant groups with the same macroscopic features were obtained (L1-L2-SBAE) to promote specific surface characteristics. Scanning electron microscopy, profilometric evaluation, X-ray spectrometry, and diffraction analysis were performed. For each group, six implants were placed in the tibiae of three Peli Buey sheep, and histologic, histomorphometric analysis, bone to implant contact (BIC), and the Dynamic Osseointegration index (DOI) were performed. Results: During the early phases of osseointegration, the histological and histomorphometric results showed significant differences between L1-L2-SBAE implants. At 15 and 30 days, histological analysis detected a newly bone formation around all specimens with an higher vital bone in L2 compared to L1 and SBAE both in cortical and in poor-quality marrow bone. At same time, histomorphometric analysis showed significantly higher BIC values in L2 (42.1 ± 2.6 and 82.4 ± 2.2) compared to L1 (5.2 ± 3.1 and 56.2 ± 1.3) and SBAE (23.3 ± 3.9 and 77.3 ± 0.4). DOI medium value showed a higher rate in L2 (2.83) compared to SBAE (2.60) and L1 (1.91). Conclusions: With the limitations of this pilot study, it is possible to assess that the titanium surface characteristics, and not the technologies used to obtain the modification, played a crucial role during the osseointegration process. Histological, histomorphometric, BIC, and DOI evaluation showed a significantly higher rate in L2 specimens compared to others, confirming that the implant surface could increase the bone response in cortical or marrow poor quality bone during the initial phases of osseointegration.
Carbon Fiber Reinforced Polymer Composites Doped with Graphene Oxide in Light of Spectroscopic Studies
Authors: Florek Paulina , Król Magdalena , Jeleń Piotr , Mozgawa Włodzimierz
Published: April 2021

Carbon fiber reinforced polymer composites are a dynamically developing group of lightweight composites for applications in the automotive, wind energy, aerospace, and sports sectors. Interfacial connection is the weakest place in these materials. In this study, an attempt was made to improve adhesion between carbon fiber and epoxy resin. For this purpose, nanoparticles of graphene oxide were added to a polymer matrix. The results of the three-point bend test showed that the strength of samples with added graphene oxide increased. Improvement of adhesion between components, reduction of the pull-out effect and change in the method of crack propagation were observed. An attempt was made to explain this effect using spectroscopic methods, both IR and Raman. On the basis of the obtained results, chemical bonds between the individual components of the composites were identified.
Caustic Frequency in 2D Stochastic Flows Modeling Turbulence
Authors: Piterbarg Leonid I.
Published: April 2021

Stochastic flows mimicking 2D turbulence in compressible media are considered. Particles driven by such flows can collide and we study the collision (caustic) frequency. Caustics occur when the Jacobian of a flow vanishes. First, a system of nonlinear stochastic differential equations involving the Jacobian is derived and reduced to a smaller number of unknowns. Then, for special cases of the stochastic forcing, upper and lower bounds are found for the mean number of caustics as a function of Stokes number. The bounds yield an exact asymptotic for small Stokes numbers. The efficiency of the bounds is verified numerically. As auxiliary results we give rigorous proofs of the well known expressions for the caustic frequency and Lyapunov exponent in the one-dimensional model. Our findings may also be used for estimating the mean time when a 2D Riemann type partial differential equation with a stochastic forcing loses uniqueness of solutions.
Temporomandibular Disorders Slow Down the Regeneration Process of Masticatory Muscles: Transcriptomic Analysis
Authors: Sindona Cinzia , Runci Anastasi Michele , Chiricosta Luigi , Gugliandolo Agnese , Silvestro Serena , Bramanti Placido , Cascone Piero , Mazzon Emanuela
Published: April 2021

Background and Objectives: Musculoskeletal injuries represent a pathological condition due to limited joint motility and morphological and functional alterations of the muscles. Temporomandibular disorders (TMDs) are pathological conditions due to alterations in the musculoskeletal system. TMDs mainly cause temporomandibular joint and masticatory muscle dysfunctions following trauma, along with various pathologies and inflammatory processes. TMD affects approximately 15% of the population and causes malocclusion problems and common symptoms such as myofascial pain and migraine. The aim of this work was to provide a transcriptomic profile of masticatory muscles obtained from TMD migraine patients compared to control. Materials and Methods: We used Next Generation Sequencing (NGS) technology to evaluate transcriptomes in masseter and temporalis muscle samples. Results: The transcriptomic analysis showed a prevalent downregulation of the genes involved in the myogenesis process. Conclusions: In conclusion, our findings suggest that the muscle regeneration process in TMD migraine patients may be slowed, therefore therapeutic interventions are needed to restore temporomandibular joint function and promote healing processes.
Ventricular Fibrillation Recurrences in Successfully Shocked Out-of-Hospital Cardiac Arrests
Authors: Aschieri Daniela , Guerra Federico , Pelizzoni Valentina , Paolini Enrico , Stronati Giulia , Moderato Luca , Losi Giulia , Compagnucci Paolo , Coccia Michela , Casella Michela , Dello Russo Antonio , Bardy Gust H. , Capucci Alessandro
Published: April 2021

Background and Objectives: The prognostic impact of ventricular fibrillation (VF) recurrences after a successful shock in out-of-hospital cardiac arrest (OOHCA) is still poorly understood, and some evidence suggests a potential pro-arrhythmic effect of chest compressions in this setting. In the present analysis, we looked at the short-term and long-term prognosis of VF recurrences in OOHCA. And their potential association with chest compressions. Materials and Methods: The Progetto Vita, prospectively collecting data on all resuscitation efforts in the Piacenza province (Italy), was used for the present analysis. From the 461 OOHCAs found in a shockable rhythm, only those with optimal ECG tracings and good audio recordings (160) were assessed. Rhythms other than VF post-shock were analyzed five seconds after shock delivery and survival to hospital admission, hospital discharge, and long-term survival data over a 14-year follow-up were collected. Results: Population mean age was 64.4 ± 16.9 years, and 31.9% of all patients were female. Mean time to EMS arrival was 5.9 ± 4.5 min. Short- and long-term survival without neurological impairment were higher in patients without VF recurrence when compared to patients with VF recurrence, independently from the pre-induction rhythm (p < 0.001). After shock delivery, VF recurrence was higher when chest compressions were resumed early after discharge and more vigorously. Conclusions: VF recurrences after a shock could worsen short and long-term survival. The potential pro-arrhythmic effect of chest compressions should be factored in when considering the real risks and benefits of this procedure.
Numerical and Experimental Evaluation of a CFRP Fatigue Strengthening for Stringer-Floor Beam Connections in a 19th Century Riveted Railway Bridge
Authors: Jimenez-Vicaria J. David , Gomez-Pulido M. Dolores , Castro-Fresno Daniel
Published: April 2021

A local and global finite element analysis of the stringer-floor beam connection of a 19th century riveted railway bridge in Spain made of puddle iron were performed to obtain the maximum principal strains in the riveted connecting angles corresponding to bending moments from train loading on the bridge. Due to the anisotropic nature of puddle iron, the connecting angles were modelled using Hill anisotropic plasticity potential and a parametric study in the local FE model of the connection was performed. A laboratory specimen fabricated with original stringers dismantled from the railway bridge was tested to calibrate the numerical models, so the yield stress ratio that best fitted experimental results was obtained. Based on the method of constant fatigue-life diagram and modified Goodman fatigue failure criterion, it was detected that the connecting angles were prone to fatigue crack initiation, as the combination of mean stress and alternating stress amplitude at the toe of the angle fillet remained outside the infinite fatigue-life region. An innovative strengthening system based on adhesively-bonded carbon-fiber reinforced polymer (CFRP) angles was designed to prevent fatigue crack initiation in the connecting angles of the stringer-floor beam connection. Different CFRP laminate layouts were numerically evaluated and a proper configuration was obtained that reduced both the mean stress and the alternating stress amplitude in the connecting angle to shift from finite fatigue-life region to infinite fatigue-life region in the constant fatigue-life diagram. To validate the effectiveness of the proposed CFRP strengthening method, its application on a second laboratory specimen fabricated with original stringers was evaluated experimentally and compared with numerical results. The research study conducted showed that the use of adhesively-bonded CFRP angles was an effective strengthening system in reducing the stress level in the fillet region of the puddle iron connecting angles (where fatigue cracks are prone to initiate) and consequently could increase fatigue life of the stringer-floor beam connection.
13C Metabolic Flux Analysis Indicates Endothelial Cells Attenuate Metabolic Perturbations by Modulating TCA Activity
Authors: Moiz Bilal , Garcia Jonathan , Basehore Sarah , Sun Angela , Li Andrew , Padmanabhan Surya , Albus Kaitlyn , Jang Cholsoon , Sriram Ganesh , Clyne Alisa Morss
Published: April 2021

Disrupted endothelial metabolism is linked to endothelial dysfunction and cardiovascular disease. Targeted metabolic inhibitors are potential therapeutics; however, their systemic impact on endothelial metabolism remains unknown. In this study, we combined stable isotope labeling with 13C metabolic flux analysis (13C MFA) to determine how targeted inhibition of the polyol (fidarestat), pentose phosphate (DHEA), and hexosamine biosynthetic (azaserine) pathways alters endothelial metabolism. Glucose, glutamine, and a four-carbon input to the malate shuttle were important carbon sources in the baseline human umbilical vein endothelial cell (HUVEC) 13C MFA model. We observed two to three times higher glutamine uptake in fidarestat and azaserine-treated cells. Fidarestat and DHEA-treated HUVEC showed decreased 13C enrichment of glycolytic and TCA metabolites and amino acids. Azaserine-treated HUVEC primarily showed 13C enrichment differences in UDP-GlcNAc. 13C MFA estimated decreased pentose phosphate pathway flux and increased TCA activity with reversed malate shuttle direction in fidarestat and DHEA-treated HUVEC. In contrast, 13C MFA estimated increases in both pentose phosphate pathway and TCA activity in azaserine-treated cells. These data show the potential importance of endothelial malate shuttle activity and suggest that inhibiting glycolytic side branch pathways can change the metabolic network, highlighting the need to study systemic metabolic therapeutic effects.
Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5
Authors: Kopejtka Karel , Zeng Yonghui , Kaftan David , Selyanin Vadim , Gardian Zdenko , Tomasch Jürgen , Sommaruga Ruben , Koblížek Michal
Published: April 2021

An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Tyrolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus Sphingomonas and has the highest pairwise 16S rRNA gene sequence similarity with Sphingomonas glacialis (98.3%), Sphingomonas psychrolutea (96.8%), and Sphingomonas melonis (96.5%). Its genomic DNA G + C content is 65.9%. Further, in silico DNA-DNA hybridization and calculation of the average nucleotide identity speaks for the close phylogenetic relationship of AAP5 and Sphingomonas glacialis. The high percentage (76.2%) of shared orthologous gene clusters between strain AAP5 and Sphingomonas paucimobilis NCTC 11030T, the type species of the genus, supports the classification of the two strains into the same genus. Strain AAP5 was found to contain C18:1ω7c (64.6%) as a predominant fatty acid (>10%) and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, six unidentified glycolipids, one unidentified phospholipid, and two unidentified lipids. The main respiratory quinone was ubiquinone-10. Strain AAP5 is a facultative photoheterotroph containing type-2 photosynthetic reaction centers and, in addition, contains a xathorhodopsin gene. No CO2-fixation pathways were found.
CD4+ and CD8+ Circulating Memory T Cells Are Crucial in the Protection Induced by Vaccination with Salmonella Typhi Porins
Authors: Ontiveros-Padilla Luis , García-Lozano Alberto , Tepale-Segura Araceli , Rivera-Hernández Tania , Pastelin-Palacios Rodolfo , Isibasi Armando , Arriaga-Pizano Lourdes A. , Bonifaz Laura C. , López-Macías Constantino
Published: April 2021

Salmonella enterica serovar Typhi (S. Typhi) porins, OmpC and OmpF, are potent inducers of the immune response against S. Typhi in mice and humans. Vaccination with porins induces the protection against 500 LD50 of S. Typhi, life-lasting bactericidal antibodies and effector T cell responses in mice; however, the nature of the memory T cell compartment and its contribution to protection remains unknown. In this work, we firstly observed that vaccination with porins induces in situ (skin) CD4+ and CD8+ T cell responses. Analysis of the porin-specific functional responses of skin CD4+ and CD8+ T cells showed IFN-gamma- and IL-17-producing cells in both T cell populations. The memory phenotype of porin-specific T cells indicated the presence of resident and effector memory phenotypes in the skin, and a central memory phenotype in the skin-draining lymph node. In addition, we demonstrated that vaccination with porins via skin reduces the bacterial burden following challenge. Finally, evaluating the role of the circulating T cell memory population in protection, we showed that circulating memory CD4+ and CD8+ T cells are crucial in porin-mediated protection against S. Typhi. Overall, this study highlights the importance of inducing circulating memory T cell responses in order to achieve the optimal protection provided by porins, showing a mechanism that could be sought in the rational development of vaccines.
Screening of Lactic Acid Bacteria for the Bio-Control of Botrytis cinerea and the Potential of Lactiplantibacillus plantarum for Eco-Friendly Preservation of Fresh-Cut Kiwifruit
Authors: De Simone Nicola , Capozzi Vittorio , de Chiara Maria Lucia Valeria , Amodio Maria Luisa , Brahimi Samira , Colelli Giancarlo , Drider Djamel , Spano Giuseppe , Russo Pasquale
Published: April 2021

Botrytis cinerea, responsible for grey mold, represents the first biological cause of fruit and vegetable spoilage phenomena in post-harvest. Kiwifruit is a climacteric fruit particularly prone to this mold infestation during storage. Lactic acid bacteria (LAB) are food-grade bacteria that can synthesize several metabolites with antimicrobial activity and are, therefore, suggested as promising and eco-friendly resources for the bio-control of molds on fruits and vegetables. In this work, we propose the screening of a collection of 300 LAB previously isolated from traditional sourdoughs for their ability to counteract in vitro the growth of Botrytis cinerea CECT 20973. Only 2% of tested LAB strains belonging to Lactiplantibacillus plantarum species, exerted a strong antagonism against B. cinerea. The cell-free supernatants were partially characterized and results clearly indicated that high levels of lactic acid contributed to the antagonistic activity. PAN01 and UFG 121 cell-free supernatants were investigated as potential bio-control agents in a preliminary in vivo assay using freshly cut kiwifruits as a food model. The application of cell-free supernatants allowed to delay the growth of B. cinerea on artificially contaminated kiwifruits until two weeks. The antagonistic activity was greatly affected by the storage temperature (25 °C and 4 °C) selected for the processed fruits, suggesting the importance to include microbial-based solution in a broader framework of hurdle technologies.
Editorial for Special Issue “Reutilization and Valorization of Mine Waste”
Authors: Demers Isabelle , Coudert Lucie
Published: April 2021

Solid waste management is the most important environmental challenge of mining operations worldwide [...]
UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities
Authors: Parra Claudio , Muñoz Patricio , Bustos Luis , Parra Felipe , Simirgiotis Mario J. , Escobar Hugo
Published: April 2021

The Lamiaceae family is an important source of species among medicinal plants highly valued for their biological properties and numerous uses in folk medicine. Origanum is one of the main genera that belong to this family. The purpose of the study was to determine the phenolic composition of the Origanum vulgare extract and evaluate the antimicrobial, antioxidant, and inhibitory activities of this species that grows in the Andean region of the Atacama Desert. High-performance liquid chromatography was performed to determine the main phenols. Rosmarinic acid was identified as the predominant phenolic compound in this species (76.01 mg/100 g DW), followed by protocatechuic acid, which to our knowledge, no previous study reported similar concentrations in O. vulgare. The oregano extract exhibited a content of total phenolic (3948 mg GAE/100 g DW) and total flavonoid (593 mg QE/100 g DW) with a higher DPPH antioxidant activity (IC50 = 40.58 µg/mL), compared to the same species grown under other conditions. Furthermore, it was found to inhibit α-glucosidase activity with an IC50 value (7.11 mg/mL) lower than acarbose (129.32 mg/mL). Pseudomonas syringae and Pantoea agglomerans (both MIC 0.313 mg/mL and MBC 1.25 mg/mL) were the bacteria most susceptible to oregano extract with the lowest concentration necessary to inhibit bacterial growth. These results open the door for the potential use of this plant to manage chronic diseases, and they expand the knowledge of the species cultivated in arid environmental conditions.
Interaction of Squid (Dosidicus giga) Mantle Protein with a Mixtures of Potato and Corn Starch in an Extruded Snack, as Characterized by FTIR and DSC
Authors: Valenzuela-Lagarda José Luis , Pacheco-Aguilar Ramón , Gutiérrez-Dorado Roberto , Mendoza Jaime Lizardi , López-Valenzuela Jose Ángel , Mazorra-Manzano Miguel Ángel , Muy-Rangel María Dolores
Published: April 2021

The majority of snacks expanded by extrusion (SEE) are made with vegetable sources, to improve their nutritional content; it has been proposed to incorporate squid (Dosidicus gigas), due to its high protein content, low price and high availability. However, the interaction of proteins of animal origin with starch during extrusion causes negative effects on the sensory properties of SEE, so it is necessary to know the type of protein–carbohydrate interactions and their effect on these properties. The objective of this research was to study the interaction of proteins and carbohydrates of SEE elaborated with squid mantle, potato and corn. The nutritional composition and protein digestibility were evaluated, Fourier transform infrared (FTIR) and Differential Scanning Calorimetry (DSC) were used to study the formation of protein–starch complexes and the possible regions responsible for their interactions. The SEE had a high protein content (40–85%) and biological value (>93%). The melting temperature (Tm) was found between 145 and 225 °C; the Tm values in extruded samples are directly proportional to the squid content. The extrusion process reduced the amine groups I and II responsible for the protein–protein interaction and increased the O-glucosidic bonds, so these bonds could be responsible for the protein–carbohydrate interactions.
Hydroxytyrosol and Oleuropein-Enriched Extracts Obtained from Olive Oil Wastes and By-Products as Active Antioxidant Ingredients for Poly (Vinyl Alcohol)-Based Films
Authors: Luzi Francesca , Pannucci Elisa , Clemente Mariangela , Grande Edoardo , Urciuoli Silvia , Romani Annalisa , Torre Luigi , Puglia Debora , Bernini Roberta , Santi Luca
Published: April 2021

Oxidative stability of food is one of the most important parameters affecting integrity and consequently nutritional properties of dietary constituents. Antioxidants are widely used to avoid deterioration during transformation, packaging, and storage of food. In this paper, novel poly (vinyl alcohol) (PVA)-based films were prepared by solvent casting method adding an hydroxytyrosol-enriched extract (HTyrE) or an oleuropein-enriched extract (OleE) in different percentages (5, 10 and 20% w/w) and a combination of both at 5% w/w. Both extracts were obtained from olive oil wastes and by-products using a sustainable process based on membrane technologies. Qualitative and quantitative analysis of each sample carried out by high performance liquid chromatography (HPLC) and nuclear resonance magnetic spectroscopy (NMR) proved that the main components were hydroxytyrosol (HTyr) and oleuropein (Ole), respectively, two well-known antioxidant bioactive compounds found in Olea europaea L. All novel formulations were characterized investigating their morphological, optical and antioxidant properties. The promising performances suggest a potential use in active food packaging to preserve oxidative-sensitive food products. Moreover, this research represents a valuable example of reuse and valorization of agro-industrial wastes and by-products according to the circular economy model.
Distinguished Contributions in the Fields of Biomedical and Environmental Applications Incorporating Nanostructured Materials and Composites in Journal Molecules
Authors: Stylianakis Minas M.
Published: April 2021

During the last two years, over 10,000 papers (articles, reviews, communications etc.) were published in Molecules [...]
Identification of Flavonoids as Putative ROS-1 Kinase Inhibitors Using Pharmacophore Modeling for NSCLC Therapeutics
Authors: Parate Shraddha , Kumar Vikas , Hong Jong Chan , Lee Keun Woo
Published: April 2021

Non-small cell lung cancer (NSCLC) is a lethal non-immunogenic malignancy and proto-oncogene ROS-1 tyrosine kinase is one of its clinically relevant oncogenic markers. The ROS-1 inhibitor, crizotinib, demonstrated resistance due to the Gly2032Arg mutation. To curtail this resistance, researchers developed lorlatinib against the mutated kinase. In the present study, a receptor-ligand pharmacophore model exploiting the key features of lorlatinib binding with ROS-1 was exploited to identify inhibitors against the wild-type (WT) and the mutant (MT) kinase domain. The developed model was utilized to virtually screen the TimTec flavonoids database and the retrieved drug-like hits were subjected for docking with the WT and MT ROS-1 kinase. A total of 10 flavonoids displayed higher docking scores than lorlatinib. Subsequent molecular dynamics simulations of the acquired flavonoids with WT and MT ROS-1 revealed no steric clashes with the Arg2032 (MT ROS-1). The binding free energy calculations computed via molecular mechanics/Poisson-Boltzmann surface area (MM/PBSA) demonstrated one flavonoid (Hit) with better energy than lorlatinib in binding with WT and MT ROS-1. The Hit compound was observed to bind in the ROS-1 selectivity pocket comprised of residues from the β-3 sheet and DFG-motif. The identified Hit from this investigation could act as a potent WT and MT ROS-1 inhibitor.
A Versatile Solid-Phase Approach to the Synthesis of Oligonucleotide Conjugates with Biodegradable Hydrazone Linker
Authors: Meschaninova Mariya I. , Entelis Nina S. , Chernolovskaya Elena L. , Venyaminova Alya G.
Published: April 2021

One of the ways to efficiently deliver various drugs, including therapeutic nucleic acids, into the cells is conjugating them with different transport ligands via labile or stable bonds. A convenient solid-phase approach for the synthesis of 5′-conjugates of oligonucleotides with biodegradable pH-sensitive hydrazone covalent bonds is proposed in this article. The approach relies on introducing a hydrazide of the ligand under aqueous/organic media to a fully protected support-bound oligonucleotide containing aldehyde function at the 5′-end. We demonstrated the proof-of-principle of this approach by synthesizing 5′-lipophilic (e.g., cholesterol and α-tocopherol) conjugates of modified siRNA and non-coding RNAs imported into mitochondria (antireplicative RNAs and guide RNAs for Mito-CRISPR/system). The developed method has the potential to be extended for the synthesis of pH-sensitive conjugates of oligonucleotides of different types (ribo-, deoxyribo-, 2′-O-methylribo-, and others) with ligands of different nature.
Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes
Authors: Fical Luboš , Khalikova Maria , Kočová Vlčková Hana , Lhotská Ivona , Hadysová Zuzana , Vokřál Ivan , Červený Lukáš , Švec František , Nováková Lucie
Published: April 2021

Two new ultra-high performance liquid chromatography (UHPLC) methods for analyzing 21 selected antivirals and their metabolites were optimized, including sample preparation step, LC separation conditions, and tandem mass spectrometry detection. Micro-solid phase extraction in pipette tips was used to extract antivirals from the biological material of Hanks balanced salt medium of pH 7.4 and 6.5. These media were used in experiments to evaluate the membrane transport of antiviral drugs. Challenging diversity of physicochemical properties was overcome using combined sorbent composed of C18 and ion exchange moiety, which finally allowed to cover the whole range of tested antivirals. For separation, reversed-phase (RP) chromatography and hydrophilic interaction liquid chromatography (HILIC), were optimized using extensive screening of stationary and mobile phase combinations. Optimized RP-UHPLC separation was carried out using BEH Shield RP18 stationary phase and gradient elution with 25 mmol/L formic acid in acetonitrile and in water. HILIC separation was accomplished with a Cortecs HILIC column and gradient elution with 25 mmol/L ammonium formate pH 3 and acetonitrile. Tandem mass spectrometry (MS/MS) conditions were optimized in both chromatographic modes, but obtained results revealed only a little difference in parameters of capillary voltage and cone voltage. While RP-UHPLC-MS/MS exhibited superior separation selectivity, HILIC-UHPLC-MS/MS has shown substantially higher sensitivity of two orders of magnitude for many compounds. Method validation results indicated that HILIC mode was more suitable for multianalyte methods. Despite better separation selectivity achieved in RP-UHPLC-MS/MS, the matrix effects were noticed while using both chromatographic modes leading to signal enhancement in RP and signal suppression in HILIC.
Phytochemical Composition, Antioxidant and Antimicrobial Activity of the Balkan Endemic Micromeria frivaldszkyana (Degen) Velen. (Lamiaceae)
Authors: Mladenova Tsvetelina , Stoyanov Plamen , Denev Petko , Dimitrova Stela , Katsarova Mariana , Teneva Desislava , Todorov Krasimir , Bivolarska Anelia
Published: April 2021

The current study investigates the content of sugars, organic acids, phenolic acids and flavonoids, as well as antioxidant and antimicrobial activity of Balkan-endemic Micromeria frivaldszkyana. Glucose was the most abundant sugar in the plant (2.77%), followed by fructose (1.18%) and galactose (0.82%). Eight organic acids were detected with quinic acid being in the highest content—556.3 mg/100 g DW. From the individual phenolic acids, rosmarinic acid was found in the most significant amounts (2040.1 ± 1.97 mg/100 g) and hesperidin was the major representative of flavonoids with content 131.2 ± 5.6 mg/100 g DW. The antioxidant activity of the plant was studied by six methods: 2,2′-diphenylpicrylhydrazyl (DPPH)—286.4 ± 10.43 mM TE/g, 2,2′azinobis (3)-ethylbenzthiazoline-6-sulfonic acid (ABTS)—358.4 ± 10.4 mM TE/g, ferric reducing antioxidant power (FRAP)—388.0 ± 32.4 mM TE/g, cupric reducing antioxidant capacity (CUPRAC)—905.6 ± 19.2 mM TE/g, Oxygen Radical Absorbance Capacity (ORAC)—3250.5 ± 208.1 µmol TE/g and Hydroxyl Radical Averting Capacity (HORAC)—306.1 ± 23.5 µmol GAE/g. In vitro antimicrobial activity against nine microorganism was evaluated but the extract displayed antimicrobial activity only against Listeria monocytogenes ATCC 19111 with inhibition zone diameter 9 mm and minimal inhibitory concentration (MIC) 10 mg/mL.
WUSCHEL Overexpression Promotes Callogenesis and Somatic Embryogenesis in Medicago truncatula Gaertn
Authors: Kadri Aline , Grenier De March Ghislaine , Guerineau François , Cosson Viviane , Ratet Pascal
Published: April 2021

The induction of plant somatic embryogenesis is often a limiting step for plant multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones and the induction of specific genes. The WUSCHEL gene (WUS) is required for the production of plant embryogenic stem cells. To explore a different approach to induce somatic embryogenesis, we have investigated the effect of the heterologous ArabidopsisWUS gene overexpression under the control of the jasmonate responsive vsp1 promoter on the morphogenic responses of Medicago truncatula explants. WUS expression in leaf explants increased callogenesis and embryogenesis in the absence of growth regulators. Similarly, WUS expression enhanced the embryogenic potential of hairy root fragments. The WUS gene represents thus a promising tool to develop plant growth regulator-free regeneration systems or to improve regeneration and transformation efficiency in recalcitrant crops.
Antioxidant Enzymatic Activities and Growth Response of Quinoa (Chenopodium quinoa Willd) to Exogenous Selenium Application
Authors: Khalofah Ahlam , Migdadi Hussein , El-Harty Ehab
Published: April 2021

Selenium is a trace element essential to many organisms, including higher plants. At low concentrations, it enhances growth and development; however, it is toxic at high concentrations. The development of crops with proper levels of selenium will be worth for both nutrition and Se-based therapeutics. This study aimed to investigate the morphological, physiological, and biochemical responses of the quinoa plant to 0, 2.5, 5, 10, and 20 mg/L of Na2SeO3·5H2O. Selenium at low concentrations (2.5 and 5 mg/L), quinoa plant showed a significant increase of growth parameters, relative water content, photosynthetic pigments, proline, total soluble sugars, and antioxidant enzymes activities as (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD, ascorbate peroxidase (APX), and glutathione reductase (GR)), and contents of malondialdehyde (MDA) and H2O2 were reduced. However, high concentrations (10 and 20) mg/L caused a decrease in plant growth parameters, relative water content, and photosynthetic pigments. In contrast, excess selenium increased the oxidative stress monitored by hydrogen peroxide and lipid peroxidation levels. The enzymatic antioxidant system responded to the selenium supply significantly increased. Osmolytes compounds, such as total sugars and proline, increased in selenium-treated plants. The increase in these osmolytes compounds may show a defense mechanism for the osmotic readjustment of quinoa plants to mitigate the toxicity caused by selenium. This study shows the morphological and physiological responses that must be considered for success in the sustainable cultivation of quinoa plants in environments containing excess selenium.
Moment Redistribution in Continuous Externally CFRP Prestressed Beams with Steel and FRP Rebars
Authors: Lou Tiejiong , Li Zhangxiang , Pang Miao
Published: April 2021

This paper assesses the impact of adopting carbon- or glass-fiber-reinforced polymer (CFRP or GFRP) instead of steel rebars on the redistribution of moments in prestressed concrete beams (PCBs) with external CFRP tendons. A numerical program is introduced, and numerical simulations are performed on two-span continuous beams with steel, CFRP or GFRP rebars of various areas, i.e., Ar2 = 360–3560 mm2, and Ar1/Ar2 = 1.5, where Ar1 and Ar2 are areas of tensile rebars over the positive and negative moment zones, respectively. The results show the moment redistribution is contributed by concrete cracking only for the beams with fiber-reinforced polymer (FRP) rebars, and by concrete cracking and steel yielding for the beams with steel rebars. As a result, the use of FRP rebars leads to a substantially lower moment redistribution than in steel rebars. It is also demonstrated that Eurocode 2, CSA A23.3-04 and ACI 318-19 fail to reflect the rebar influence on moment redistribution in PCBs with external tendons. A simplified equation for the quantification of moment redistribution in externally PCBs with steel and FRP rebars is recommended, which yields accurate and conservative predictions.