bluuebunny/crossref_metadata_2025 huggingface dataset:
Convergent functional changes of the episodic memory impairment in mild cognitive impairment: An ALE meta-analysis
Authors: Liang Xuhong , Yuan Qianqian , Xue Chen , Qi Wenzhang , Ge Honglin , Yan Zheng , Chen Shanshan , Song Yu , Wu Huimin , Xiao Chaoyong , Chen Jiu
Published: July 2022

Background

Mild cognitive impairment (MCI) is considered to be an intermediate stage between normal aging and Alzheimer's disease (AD). The earliest and most common symptom of MCI is impaired episodic memory. When episodic memory is impaired in MCI patients, specific functional changes occur in related brain areas. However, there is currently a lack of a unified conclusion on this change. Therefore, the purpose of this meta-analysis is to find MRI-specific functional changes in episodic memory in MCI patients.

Methods

Based on three commonly used indicators of brain function: functional connectivity (FC), the amplitude of low-frequency fluctuation /fractional amplitude of low-frequency fluctuation (ALFF/fALFF), and regional homogeneity (ReHo), we systematically searched PubMed, Web of Science and Ovid related literature and conducted the strict screening. Then we use the activation likelihood estimation (ALE) algorithm to perform the coordinate-based meta-analysis.

Results

Through strict screening, this meta-analysis finally included 21 related functional neuroimaging research articles. The final result displays that functional changes of episodic memory in MCI patients are mainly located in the parahippocampal gyrus, precuneus, posterior cingulate gyrus, cuneus, middle temporal gyrus, middle frontal gyrus, lingual gyrus, and thalamus.

Conclusions

There are specific functional changes in episodic memory brain regions in MCI patients, and the brain functional network can regulate episodic memory through these brain regions. And these specific changes can assist in the early diagnosis of MCI, providing new ideas and directions for early identification and intervention in the process of MCI.
Auditory Manifestations of Vestibular Migraine
Authors: Shi Suming , Wang Dan , Ren Tongli , Wang Wuqing
Published: July 2022

Objectives

To investigate the auditory features of patients with vestibular migraine (VM) and to analyze the possible relevant factors of hearing loss.

Methods

A total of 166 patients with VM were enrolled. Demographic variables, age of onset, disease course, distribution of vestibular attacks, characteristics of hearing loss, and the coexistence of related disorders, such as visual aura, familial history, motion sickness, nausea, headache, photophobia, otalgia, tinnitus, aural fullness, and phonophobia, were analyzed and compared.

Results

Patients with VM can manifest otalgia (8.4%), tinnitus (51.8%), aural fullness (41%), and phonophobia (31.9%). Of 166 patients, the prevalence of VMw was 21.1% (n = 35). Patients with VMw mainly manifested mild and easily reversible low-frequency hearing loss. The proportions of tinnitus and aural fullness were significantly larger in patients with VMw than that in patients with VMo (P < 0.05). The duration of vestibular symptoms was significantly shorter in patients with VMw (P < 0.05). However, the age of onset, disease course, gender, frequency of vestibular attacks, the coexistence of visual aura, familial history, motion sickness, nausea, headache, photophobia, otalgia, and phonophobia had no significant difference between the two groups.

Conclusion

Auditory symptoms were common in patients with VM. The hearing loss of VM was characterized by a mild and easily reversible low-frequency hearing loss, accompanied by higher proportions of tinnitus and aural fullness, and a shorter duration of vestibular symptoms compared with patients with VMo.
Alpha rhythm of electroencephalography was modulated differently by three transcranial direct current stimulation protocols in patients with ischemic stroke
Authors: Chen Yuanyuan , Wang Chunfang , Song Peiqing , Sun Changcheng , Zhang Ying , Zhao Xin , Du Jingang
Published: July 2022

The heterogeneity of transcranial direct current stimulation (tDCS) protocols and clinical profiles may explain variable results in modulating excitability in the motor cortex after stroke. However, the cortical electrical effects induced by different tDCS protocols remain unclear. Here, we aimed to compare rhythm changes in electroencephalography (EEG) induced by three tDCS position protocols and the association between tDCS effects and clinical factors in stroke. Nineteen patients with chronic ischemic stroke underwent four experimental sessions with three tDCS protocols [anodal (atDCS), cathodal (ctDCS), and bilateral (bi-tDCS)] and a sham protocol, according to a single-blind randomized crossover design. Resting-state EEG was acquired before and after each protocol. First, a paired-sample t-test was used to examine the difference in spectral power between pre- and post-stimulation. Then, linear and quadratic regression models were used separately to describe the association between the clinical factors of stroke and changes in spectral power which was significantly different between pre- and post-tDCS. Finally, repeated measures analysis of variance with lesion hemisphere, stimulation protocol, and the location was performed to investigate the effects of tDCS over time. The induced effect of tDCS was mainly reflected in the alpha rhythms. The alpha power was increased by atDCS, especially low-alpha (8–10 Hz), in localized areas of the central and distant areas of the frontal and parietal lobes. Bi-tDCS also affected alpha power but in a smaller area that mainly focused on high-alpha rhythms (10–13 Hz). However, ctDCS and sham had no significant effects on any EEG rhythm. The clinical factors of time since stroke and motor impairment level were related to the change in high-alpha induced by atDCS and bi-tDCS following quadratic regression models. The above-mentioned modulation effect lasted for 20 min without attenuation. In conclusion, our findings provide evidence that the alpha rhythm of EEG is modulated differently by different tDCS protocols and that high alpha is affected by clinical characteristics such as post-stroke time and motor deficits, which is of great significance for understanding the modulation effect of different tDCS protocols on stroke and the guidance of protocols to promote motor recovery following stroke.
Neurotrophin Crosstalk in the Etiology and Treatment of Neuropsychiatric and Neurodegenerative Disease
Authors: Joshi Rajeev , Salton Stephen R. J.
Published: July 2022

This article reviews the current progress in our understanding of the mechanisms by which growth factors, including brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF), and select neurotrophin-regulated gene products, such as VGF (non-acronymic) and VGF-derived neuropeptides, function in the central nervous system (CNS) to modulate neuropsychiatric and neurodegenerative disorders, with a discussion of the possible therapeutic applications of these growth factors to major depressive disorder (MDD) and Alzheimer’s disease (AD). BDNF and VEGF levels are generally decreased regionally in the brains of MDD subjects and in preclinical animal models of depression, changes that are associated with neuronal atrophy and reduced neurogenesis, and are reversed by conventional monoaminergic and novel ketamine-like antidepressants. Downstream of neurotrophins and their receptors, VGF was identified as a nerve growth factor (NGF)- and BDNF-inducible secreted protein and neuropeptide precursor that is produced and trafficked throughout the CNS, where its expression is greatly influenced by neuronal activity and exercise, and where several VGF-derived peptides modulate neuronal activity, function, proliferation, differentiation, and survival. Moreover, levels of VGF are reduced in the CSF of AD subjects, where it has been repetitively identified as a disease biomarker, and in the hippocampi of subjects with MDD, suggesting possible shared mechanisms by which reduced levels of VGF and other proteins that are similarly regulated by neurotrophin signaling pathways contribute to and potentially drive the pathogenesis and progression of co-morbid neuropsychiatric and neurodegenerative disorders, particularly MDD and AD, opening possible therapeutic windows.
Biopolymeric nanoparticles based effective delivery of bioactive compounds toward the sustainable development of anticancerous therapeutics
Authors: Pathak Neelam , Singh Pankaj , Singh Pradeep Kumar , Sharma Swati , Singh Rajat Pratap , Gupta Anmol , Mishra Richa , Mishra Vivek Kumar , Tripathi Manikant
Published: July 2022

Nowadays, effective cancer therapy is a global concern, and recent advances in nanomedicine are crucial. Cancer is one of the major fatal diseases and a leading cause of death globally. Nanotechnology provides rapidly evolving delivery systems in science for treating diseases in a site-specific manner using natural bioactive compounds, which are gaining widespread attention. Nanotechnology combined with bioactives is a very appealing and relatively new area in cancer treatment. Natural bioactive compounds have the potential to be employed as a chemotherapeutic agent in the treatment of cancer, in addition to their nutritional benefits. Alginate, pullulan, cellulose, polylactic acid, chitosan, and other biopolymers have been effectively used in the delivery of therapeutics to a specific site. Because of their biodegradability, biopolymeric nanoparticles (BNPs) have received a lot of attention in the development of new anticancer drug delivery systems. Biopolymer-based nanoparticle systems can be made in a variety of ways. These systems have developed as a cost-effective and environmentally friendly solution to boost treatment efficacy. Effective drug delivery systems with improved availability, increased selectivity, and lower toxicity are needed. Recent research findings and current knowledge on the use of BNPs in the administration of bioactive chemicals in cancer therapy are summarized in this review.
Glucometabolic reprogramming: From trigger to therapeutic target in hepatocellular carcinoma
Authors: Xia Haoming , Huang Ziyue , Wang Zhensheng , Liu Shuqiang , Zhao Xudong , You Junqi , Xu Yi , Yam Judy Wai Ping , Cui Yunfu
Published: July 2022

Glucose, the central macronutrient, releases energy as ATP through carbon bond oxidation and supports various physiological functions of living organisms. Hepatocarcinogenesis relies on the bioenergetic advantage conferred by glucometabolic reprogramming. The exploitation of reformed metabolism induces a uniquely inert environment conducive to survival and renders the hepatocellular carcinoma (HCC) cells the extraordinary ability to thrive even in the nutrient-poor tumor microenvironment. The rewired metabolism also confers a defensive barrier which protects the HCC cells from environmental stress and immune surveillance. Additionally, targeted interventions against key players of HCC metabolic and signaling pathways provide promising prospects for tumor therapy. The active search for novel drugs based on innovative mutation targets is warranted in the future for effectively treating advanced HCC and the preoperative downstage. This article aims to review the regulatory mechanisms and therapeutic value of glucometabolic reprogramming on the disease progression of HCC, to gain insights into basic and clinical research.
Comparison of high flow nasal cannula and non-invasive positive pressure ventilation in children with bronchiolitis: A meta-analysis of randomized controlled trials
Authors: Zhong Zhaoshuang , Zhao Long , Zhao Yan , Xia Shuyue
Published: July 2022

Background

The effects of high-flow nasal cannula (HFNC) compared to non-invasive positive pressure ventilation (NIPPV) on children with bronchiolitis remain unclear.

Methods

This meta-analysis was performed following the preferred reporting items for systematic reviews and meta-analysis (PRISMA) statement. Randomized controlled trials (RCTs) were identified from a comprehensive search in PubMed, EMBASE, Cochrane Library, and Web of Science without time and language limitations. Primary endpoints include the rate of treatment failure, the rate of need for intubation, and the pediatric intensive care unit (PICU) length of stay.

Results

Five RCTs including 541 children of less than 24 months were enrolled in the meta-analysis. Compared to the NIPPV group, the rate of treatment failure was significantly higher in the HFNC treatment group (I2 = 0.0%, P = 0.574; RR 1.523, 95% CI 1.205 to 1.924, P < 0.001). No significant difference was noted in the need for intubation (I2 = 0.0%, P = 0.431; RR 0.874, 95% CI 0.598 to 1.276, P = 0.485) and the PICU length of stay (I2 = 0.0%, P = 0.568; WMD = –0.097, 95% CI = –0.480 to 0.285, P = 0.618) between the HFNC group and the NIPPV treatment.

Conclusion

Compared to the NIPPV group, HFNC therapy was associated with a significantly higher treatment failure rate in children suffering from bronchiolitis. The intubation rate and the PICU length of stay were comparable between the two approaches.
Zi Shen Wan Fang Attenuates Neuroinflammation and Cognitive Function Via Remodeling the Gut Microbiota in Diabetes-Induced Cognitive Impairment Mice
Authors: Shi Jiangwei , Yin Qingsheng , Zhang Lin , Wu Yu , Yi Pengrong , Guo Mengqing , Li Huhu , Yuan Liuyi , Wang Zixuan , Zhuang Pengwei , Zhang Yanjun
Published: July 2022

Background: Cognitive dysfunction is a critical complication of diabetes mellitus, and there are still no clinically approved drugs. Zi Shen Wan Fang (ZSWF) is an optimized prescription composed of Anemarrhenae Rhizoma, Phellodendri Chinensis Cortex, and Cistanches Herba. The purpose of this study is to investigate the effect of ZSWF on DCI and explore its mechanism from the perspective of maintaining intestinal microbial homeostasis in order to find an effective prescription for treating DCI.

Methods: The diabetes model was established by a high-fat diet combined with intraperitoneal injections of streptozotocin (STZ, 120 mg/kg) and the DCI model was screened by Morris water maze (MWM) after 8 weeks of continuous hyperglycemic stimulation. The DCI mice were randomly divided into the model group (DCI), the low- and high-ZSWF–dose groups (9.63 g/kg, 18.72 g/kg), the mixed antibiotic group (ABs), and the ZSWF combined with mixed antibiotic group (ZSWF + ABs). ZSWF was administered orally once a day for 8 weeks. Then, cognitive function was assessed using MWM, neuroinflammation and systemic inflammation were analyzed by enzyme-linked immunosorbent assay kits, intestinal barrier integrity was assessed by hematoxylin-eosin (HE) staining and Western blot and high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Furthermore, the alteration to intestinal flora was monitored by 16S rDNA sequencing.

Results: ZSWF restored cognitive function in DCI mice and reduced levels of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α. Moreover, ZSWF protected the integrity of the intestinal barrier by increasing intestinal ZO-1 and occludin protein expression and decreasing urinary lactulose to mannitol ratio. In addition, ZSWF reshaped the imbalanced gut microbiota in DCI mice by reversing the abundance changes of a wide range of intestinal bacteria at the phyla and genus levels. In contrast, removing gut microbiota with antibiotics partially eliminated the effects of ZSWF on improving cognitive function and reducing inflammation, confirming the essential role of gut microbiota in the improvement of DCI by ZSWF.

Conclusion: ZSWF can reverse cognitive impairment in DCI mice by remolding the structure of destructed gut microbiota community, which is a potential Chinese medicine prescription for DCI treatment.
Tubeimoside-1: A review of its antitumor effects, pharmacokinetics, toxicity, and targeting preparations
Authors: Wang Chang-Lin , Gao Ming-Zhou , Gao Dong-Mei , Guo Ying-Hui , Gao Zhan , Gao Xiang-Ju , Wang Jie-Qiong , Qiao Ming-Qi
Published: July 2022

Tubeimoside-1 (TBMS-1), a natural triterpenoid saponin found in traditional Chinese herbal medicine Bolbostemmatis Rhizoma, is present in numerous Chinese medicine preparations. This review aims to comprehensively describe the pharmacology, pharmacokinetics, toxicity and targeting preparations of TBMS-1, as well the therapeutic potential for cancer treatement. Information concerning TBMS-1 was systematically collected from the authoritative internet database of PubMed, Web of Science, and China National Knowledge Infrastructure applying a combination of keywords involving “tumor,” “pharmacokinetics,” “toxicology,” and targeting preparations. New evidence shows that TBMS-1 possesses a remarkable inhibitory effect on the tumors of the respiratory system, digestive system, nervous system, genital system as well as other systems in vivo and in vitro. Pharmacokinetic studies reveal that TBMS-1 is extensively distributed in various tissues and prone to degradation by the gastrointestinal tract after oral administration, causing a decrease in bioavailability. Meanwhile, several lines of evidence have shown that TBMS-1 may cause adverse and toxic effects at high doses. The development of liver-targeting and lung-targeting preparations can reduce the toxic effect of TBMS-1 and increase its efficacy. In summary, TBMS-1 can effectively control tumor treatment. However, additional research is necessary to investigate in vivo antitumor effects and the pharmacokinetics of TBMS-1. In addition, to reduce the toxicity of TBMS-1, future research should aim to modify its structure, formulate targeting preparations or combinations with other drugs.
Changing Characteristics of Pharmaceutical Prices in China Under Centralized Procurement Policy: A Multi-Intervention Interrupted Time Series
Authors: Long Hongfei , Yang Ying , Geng Xin , Mao Zongfu , Mao Zhenhua
Published: July 2022

Objective: National centralized drug procurement organized by the Chinese government currently represents the largest group purchasing organization worldwide, to establish a reasonable price formation mechanism. This study aimed to evaluate the effects of centralized procurement policy on drug price and price ratio in China.

Method: Monthly drug procurement data of public medical institutions were extracted from the national procurement database, including 11 pilot cities and 36 months from January 2018 to December 2020. Centralized procured INNs (International Nonproprietary Names) (n= 25) and their alternative INNs (n= 96) were selected as study samples. Centralized procured INNs were divided into bid-winning and non-winning products according to the bidding results. Drug price, price distribution, and price ratio were measured. Multi-intervention interrupted time series analysis was performed to estimate the policy impacts in two centralized procurement periods.

Results: The price of centralized procured INNs showed an immediate drop of 44.57% (β= -0.59,p< 0.001) at the policy implementation, among which bid-winning drugs decreased by 61.71% (β= -0.96,p< 0.001). No significant change in the price level or trends was found for non-winning products and alternative drugs in the first-year procurement period (allp-values > 0.05). During the second-year procurement period, alternative drugs in four therapeutic categories detected significant increases in the price level (allp-values < 0.05). The overall coefficient of variation of price distribution exhibited upward trends after policy implementation. Among the most centralized procured INNs, the price ratio between certificated generics (generics that have passed the consistency evaluation) and original drugs declined significantly after policy intervention (p< 0.05), whereas the price ratio between uncertificated and certificated generics increased significantly (p< 0.05).

Conclusion: Chinese government-organized group purchasing resulted in prominent price reduction of bid-winning drugs. The policy observed a short-term “spillover” effect of synergistic price reduction, while the effect wore off after 1-year procurement period. The extremely dispersed price distribution, as well as unreasonable price ratios, requires further effective price regulation means.
Monascin abrogates RANKL-mediated osteoclastogenesis in RAW264.7 cells via regulating MAPKs signaling pathways
Authors: Cheng Yin , Liu Haixia , Li Jing , Ma Yujie , Song Changheng , Wang Yuhan , Li Pei , Chen Yanjing , Zhang Zhiguo
Published: July 2022

Osteoclasts (OCs) are multinucleated cells that play a major role in osteolytic diseases such as osteoporosis. Monascin (Ms) is one of the active substances in the traditional Chinese medicine red yeast rice. Studies have found that red yeast rice can maintain bone health. In this study, the anti-osteoclastogenesis effects of Ms on RANKL-induced RAW264.7 cells were assessed, and the underlying mechanism was investigated. Ms exhibited inhibitory effects on OC differentiation and formation in a dose-dependent manner and suppressed the bone-resorbing activity of mature OCs. Ms blocked OCs-typical genes (c-Fos, NFATc1, CSTK, MMP-9, TRAP, ITG-β3, OSCAR and DC-STAMP). Furthermore, Ms treatment considerably inhibited the activation of MAPKs, JNK and p38. Taken together, Ms suppresses RANKL-induced osteoclastogenesis of RAW264.7 cells by restraining MAPKs signaling pathways and is a potential therapeutic option as a novel OC inhibitor to mitigate bone erosion.
Certain Aspects of Quantum Transport in Zigzag Graphene Nanoribbons
Authors: Pratap Surender , Kumar Sandeep , Singh Ravi Pratap
Published: July 2022

We have investigated the Fano factor and shot noise theoretically in the confined region of the potential well of zigzag graphene nanoribbon (ZGNR). We have found that the Fano factor is approximately 1, corresponding to the minimum conductivity (σ) for both symmetrical and asymmetrical potential wells. The conductivity plot with respect to Fermi energy appears as symmetrical plateaus on both sides of zero Fermi energy. Moreover, a peak observed at zero Fermi energy in the local density of states (LDOS) confirms the edge states in the system. The transmission properties of ZGNR in the confined region of the potential well are examined using the standard tight-binding Green’s function approach. The perfect transmission observed in the confined region of the potential well shows that pnp type transistors can be made with ZGNR. We have discussed the Fano factor, shot noise, conductivity, and nanohub results in the continuation of previous results. Our results show that the presence of van-Hove singularities in the density of states (DOS) matters in the presence of edge states. The existence of these edge states is sensitive to the number of atoms considered and the nature of the potential wells. We have compared our numerical results with the results obtained from the nanohub software (CNTbands) of Purdue University.
Drosophila Populations Reared Under Tropical Semi-natural Conditions Evolve Season-dependent Differences in Timing of Eclosion
Authors: Dani Chitrang , Sheeba Vasu
Published: July 2022

Circadian clocks are considered an evolutionary adaptation to environmental cycles, helping organisms to adapt to daily and seasonal changes. However, most studies on the evolution of circadian rhythms have been carried out in controlled laboratory conditions; hence evolution of circadian clocks and rhythms in organisms reared under the influence of naturally varying time cues is not well understood. To address this, we reared large outbred fly populations in an outdoor enclosure on our institutional grounds in Bengaluru, southern India for about 150 generations, at the same time maintaining their ancestral control populations under standard laboratory conditions. Studying their rhythms in eclosion, a vital behavior for Drosophila, in the laboratory and semi-natural environments revealed that flies reared under semi-natural conditions differed in the timing of eclosion under semi-natural conditions in a season-dependent manner from their laboratory-reared counterparts. These differences were manifested under harsh semi-natural environments but not under mild ones or in standard laboratory conditions. Further analysis revealed that this phenotype might be responsive to seasonal changes in temperature cycles which was confirmed in the laboratory with simulated light and temperature cycles that approximated semi-natural conditions. Our results highlight key intricacies on the relative impact of intensity and timing of environmental cues for predicting the timing of Drosophila eclosion under tropical naturalistic conditions. Overall, our research uncovers previously unexplored aspects of adaptive circadian timekeeping in complex natural conditions, offering valuable insight into the evolution of clocks.
Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense
Authors: Wu Jing , Meng Xiaoxi , Jiang Weimin , Wang Zhaojian , Zhang Jing , Meng Fei , Yao Xiaoyan , Ye Mengjuan , Yao Liang , Wang Longhai , Yu Nianjun , Peng Daiyin , Xing Shihai
Published: July 2022

The lysine crotonylation of histone proteins is a newly identified posttranslational modification with diversified cellular functions. However, there are few reports on lysine crotonylation of non-histone proteins in medicinal plant cells. By using high-resolution liquid chromatography–mass spectrometry (LC-MS) coupled with highly sensitive-specific immune-affinity antibody analysis, a whole crotonylation proteome analysis of Dendrobium huoshanense was performed. In total, 1,591 proteins with 4,726 lysine crotonylation sites were identified; among them, 11 conserved motifs were identified. Bioinformatic analyses linked crotonylated proteins to the drought stress response and multiple metabolic pathways, including secondary metabolite biosynthesis, transport and catabolism, energy production and conversion, carbohydrate transport and metabolism, translation, and ribosomal structure and biogenesis. This study contributes toward understanding the regulatory mechanism of polysaccharide biosynthesis at the crotonylation level even under abiotic stress.
Physiological Measurements and Transcriptome Survey Reveal How Semi-mangrove Clerodendrum inerme Tolerates Saline Adversity
Authors: Liang Minting , Hu Feng , Xie Dongsheng , Chen Zhibin , Zheng Qingzhi , Xie Qiyun , Zheng Feng , Liu Dongming , Jian Shuguang , Chen Hongfeng , Liu Xuncheng , Wang Faguo
Published: July 2022

Salinity adversity has been a major environmental stressor for plant growth and reproduction worldwide. Semi-mangrove Clerodendrum inerme, a naturally salt-tolerant plant, can be studied as a successful example to understand the biological mechanism of saline resistance. Since it is a sophisticated and all-round scale process for plants to react to stress, our greenhouse study interpreted the response of C. inerme to salt challenge in the following aspects: morphology, osmotic protectants, ROS production and scavenging, ion homeostasis, photosynthetic efficiency, and transcriptome reprogramming. The results drew an overview picture to illustrate the tolerant performance of C. inerme from salt acclimatization (till medium NaCl level, 0.3 mol/L) to salinity stress (high NaCl level, 0.5 mol/L). The overall evaluation leads to a conclusion that the main survival strategy of C. inerme is globally reshaping metabolic and ion profiles to adapt to saline adversity. These findings uncover the defense mechanism by which C. inerme moderates its development rate to resist the short- and long-term salt adversity, along with rebalancing the energy allocation between growth and stress tolerance.
A Chromosome Level Genome Assembly of a Winter Turnip Rape (Brassica rapa L.) to Explore the Genetic Basis of Cold Tolerance
Authors: Wu Junyan , Xu Xin-Dong , Liu Lijun , Ma Li , Pu Yuanyuan , Wang Wangtian , Hua Xue-Yang , Song Jia-Ming , Liu Kede , Lu Guangyuan , Fang Yan , Li Xuecai , Sun Wancang
Published: July 2022

Winter rapeseed (Brassica rapa L.) is an important overwintering oilseed crop that is widely planted in northwest China and suffers chronic low temperatures in winter. So the cold stress becomes one of the major constraints that limit its production. The currently existing genomes limit the understanding of the cold-tolerant genetic basis of rapeseed. Here we assembled a high-quality long-read genome of B. rapa “Longyou-7” cultivar, which has a cold-tolerant phenotype, and constructed a graph-based pan-genome to detect the structural variations within homologs of currently reported cold-tolerant related genes in the “Longyou-7” genome, which provides an additional elucidation of the cold-tolerant genetic basis of “Longyou-7” cultivar and promotes the development of cold-tolerant breeding in B. rapa.
Root-Associated Microbiota Response to Ecological Factors: Role of Soil Acidity in Enhancing Citrus Tolerance to Huanglongbing
Authors: Li Bo , Wang Yanan , Hu Tongle , Qiu Dewen , Francis Frédéric , Wang Shuangchao , Wang Shutong
Published: July 2022

The citrus orchards in southern China are widely threatened by low soil pH and Huanglongbing (HLB) prevalence. Notably, the lime application has been used to optimize soil pH, which is propitious to maintain root health and enhance HLB tolerance of citrus; however, little is known about the interactive effects of soil acidity on the soil properties and root-associated (rhizoplane and endosphere) microbial community of HLB-infected citrus orchard. In this study, the differences in microbial community structures and functions between the acidified and amended soils in the Gannan citrus orchard were investigated, which may represent the response of the host-associated microbiome in diseased roots and rhizoplane to dynamic soil acidity. Our findings demonstrated that the severity of soil acidification and aluminum toxicity was mitigated after soil improvement, accompanied by the increase in root activity and the decrease of HLB pathogen concentration in citrus roots. Additionally, the Illumina sequencing-based community analysis showed that the application of soil amendment enriched functional categories involved in host-microbe interactions and nitrogen and sulfur metabolisms in the HLB-infected citrus rhizoplane; and it also strongly altered root endophytic microbial community diversity and structure, which represented by the enrichment of beneficial microorganisms in diseased roots. These changes in rhizoplane-enriched functional properties and microbial composition may subsequently benefit the plant's health and tolerance to HLB disease. Overall, this study advances our understanding of the important role of root-associated microbiota changes and ecological factors, such as soil acidity, in delaying and alleviating HLB disease.
Innovating Empirical Research on Legitimacy: Repertory Grid Analysis
Authors: Osterberg-Kaufmann Norma
Published: July 2022

This article uses a study on the impact of political (EU-specific) knowledge on perceptions of the legitimacy of the European Union to demonstrate the potential of the repertory grid method in the social sciences. The first objective of the study is methodological. The aim is to test the added value of the repertory grid method for surveying attitudes toward contested concepts, such as legitimacy. To this end, the influence of political knowledge on the perception of the legitimacy of the European Union is surveyed with the help of repertory grid interviews. Second, possible research questions on the effect of political knowledge on perceptions of legitimacy are developed, using the results of the present repertory grid study as a guidepost in this still underdeveloped field of research. The data collected in this preliminary study provide evidence that the importance of democratic norms and values for the evaluation of the legitimacy of national and European institutions is increasing, but at the same time critique of the political system is also increasing and the perception of legitimacy is thus decreasing.
Coping With the Emotional Impact of Working in Cancer Care: The Importance of Team Working and Collective Processing
Authors: Cotter Pádraig , Holden Anneka , Johnson Caroline , Noakes Sarah , Urch Catherine , King Alex
Published: July 2022

Hospitals provide the vast majority of cancer care. A necessary focus on survival has meant that they are less well-developed in terms of supporting patients with the emotional impact of cancer; and in supporting the frontline staff who contend with this. An integration of psychotherapeutic and neurobiological findings is used to develop an understanding of the patient-staff relationship and impact of high levels of distress within it. This includes reference to Transference and Countertransference, Mirror Neurons and Poly Vagal Theory. This paper considers how patients can unconsciously “transfer” emotional distress on to healthcare practitioners; and how this evokes an emotional response from the practitioner via the mirror neuron system (MNS). This can allow the practitioner to “feel into” the patient’s experience and develop a more nuanced understanding. However, it may also activate emotions connected to the practitioner’s life and can leave them feeling overwhelmed. The practitioner’s capacity to regulate their own emotional arousal, via the vagus nerve, has a significant impact on their ability to support the patient and themselves within emotionally distressing interactions. This dynamic often unfolds without either party having significant awareness of it. A Systemic and Process-Oriented perspective is taken to understand this within the broader context of a hospital-based structure; and consider how practitioners on frontline teams may or may not support each other in working collectively with high levels of distress. A team’s level of understanding and attunement to emotional experiences as well their primary relational and communication style has significant bearing on capacity for emotion-and-relationship focused coping. A failure to work with the emotional and relational interconnection between patients and staff can contribute to isolated patients, disconnected staff, conflict within teams and an overarching system lacking in compassion. However, due to the often unconscious nature of such processes and limited understanding or training on them, they are regularly left unaddressed. Over time, this can have an accumulated effect on everyone. Group-based collective processing is considered in terms of how it can be used in supporting practitioners to integrate an emotional and relational way of working with a problem-focused approach and integrated into regular daily working.
Determinants of Unverified News Sharing on Social Media and Its Effects on Corporate Image
Authors: Zhang Zhe , Akhter Shamim , Al-Abyadh Mohammed Ali , Cong Phan The
Published: July 2022

Social media channels are interactive channels that let users spread content, participate, and generate positive or negative news. In this era of social media (SM), organizations run structured and systematic campaigns to shape their corporate images. The present study examines the role of social consciousness (SC) of employees, entertainment (Ent), and altruism (Alt) on unverified news sharing (UVN) with the mediation of corporate image (CI) and the moderation of workplace stress (WS). We conducted the study on 375 employees of the social media teams in the corporate sector in China. The sampling technique used in this study is convenience sampling. We carried out data analysis using structural equation modeling (SEM) with the help of Smart PLS (Partial Least Square) software. The results reveal that the entertainment and altruism of employees affect UVN. However, the direct negative effect of social consciousness of employees and CI on UVN came out to be insignificant. The findings also show that CI mediates the relationship between the social consciousness (SC) of employees and UVN, altruism and UVN, and entertainment and UVN. The moderating role of WS between CI and UVN is significant. Theoretically, the study contributes to the literature by examining the effect of different determinants of UVN on SM on the role of CI and WS. Practically, the present study provides implications for the managers and the organizations. The study finds that Alt is an essential factor that fosters UVN and CI; therefore, altruistic values of the employees should be instigated to reduce the flow of UVN.
Employee Engagement and Innovative Work Behavior Among Chinese Millennials: Mediating and Moderating Role of Work-Life Balance and Psychological Empowerment
Authors: Ali Hazem , Li Min , Qiu Xunmin
Published: July 2022

Given the ever-changing business environment, organizations are forced to consider innovation as an essential prerequisite to enhance their efficiency, productivity, and sustainability. In this regard, organizations pay increased attention to enhancing employees' engagement (EE) and stimulating their innovative work behaviors (IWBs). Research emphasizes the importance of employees' IWBs in achieving competitive advantages and organizational sustainability. In this research, we address the question of whether employee engagement leads to stimulating IWBs of the Chinese millennial workforce in service industries. In addition, we explore the potential mediating effect of work-life balance (WLB) and the moderating influence of psychological empowerment (PE) on the relationship between EE and IWBs. Self-administered questionnaires were used to collect data from 372 Chinese senior employees working in the IT, trade, real estate, financial, and telecommunication industries. Our empirical findings showed that highly engaged employees are most likely to exhibit IWBs and maintain a WLB. In addition, the relationship between EE and IWB was partially mediated by WLB. Moreover, the interaction between EE and PE was found to strengthen employees' IWBs. Our study contributes to understanding the importance of EE as an essential prerequisite for millennials' IWBs and provides new insights for service organizations to encourage employees' IWBs. This study contributes to the human resource management field by offering valuable implications vis-à-vis how service organizations operating in a turbulent business environment stimulate the IWBs of their millennial workforce.
Influence of Workload, Personality, and Psychological Flexibility on Occupational Stress Among Medical Staff: A Fuzzy-Set Qualitative Comparative Analysis
Authors: Quan Liming , Zhang Yang , Jiang Fugui , Liu Ying , Lan Yajia , Huang Lei
Published: July 2022

Objective

During the COVID-19 pandemic, the occupational stress of medical staff has been a major issue. This study aimed to suggest a new strategy to identify high-risk factor sets of occupational stress in medical staff using fuzzy-set qualitative comparative analysis (fs-QCA) and provide ideas for the prevention and intervention of occupational stress.

Methods

A total of 1,928 medical staff members were surveyed and tested using the Acceptance and Action Questionnaire-II (AAQ-II), Occupational Stress Inventory-Revised edition (OSI-R), and Eysenck Personality Questionnaire-Revised Short Scale (EPQ-RSC). The fs-QCA was used to explore the high-risk factors for occupational stress among medical staff.

Results

The psychological strain (PSY) score of the medical staff was 26.8 ± 7.13, and the physical strain (PHS) score was 24.3 ± 6.50. Low psychological flexibility score-introversion-high role overload, introversion-neuroticism-high role overload, and low psychological flexibility score-neuroticism were high-risk factor sets for PSY. Low psychological flexibility score-introversion-high role overload, low psychological flexibility score-introversion-neuroticism, low psychological flexibility score-neuroticism-high role overload, low psychological flexibility score-psychoticism-neuroticism, and psychoticism-neuroticism-high role overload were high-risk factor sets for PHS.

Conclusion

There are different combinations of high-risk factors for occupational stress among the medical staff. For occupational stress intervention and psychological counseling, targeted and individualized health intervention measures should be implemented according to specific characteristic combinations of different individuals.
Consumers' Perception About Front of Package Food Labels (FOPL) in India: A Survey of 14 States
Authors: Bhattacharya Sudip , Bera Om Prakash , Shah Vandana
Published: July 2022

Introduction

Front of Package Food Labels (FoP) help consumers make healthier food choices at the point of purchase by giving details about the nutrients available in the packaged food items.

Aim and Objective

A prospective multi-centric cross-sectional study was conducted in 2021 across India to evaluate the existing knowledge and attitude regarding food labels on packaged foods and beverages. Also, the objective understanding of the consumers' knowledge on different types of FoP label practiced across the world was determined.

Methodology

A self-administered questionnaire was given to the respondents to gather their attitudes regarding the FoP label. Besides, they were given colored pictures of different FoP labels to seek their perception and preference for different FoP label designs.

Results

Results found that packaged food and beverages were consumed by 91.3% of the participants. Awareness about the food package labeling was widely held by 95% of the participants and 88.6% of them considered this information helpful. Over half (55.4%) of the respondents considered packaged foods as healthy. Warning Labels (WL) were the most preferred food labels (93%), followed by Multiple Traffic Lights (MTL) and the difference between the two was statistically significant (p-value < 0.05).

Conclusions

The awareness about FoP labels is low among the consumers.

Recommendations

Evidence-based research is recommended regarding the knowledge and perception of people on the feasibility of FoP label design which may lay a foundation to formulate laws and policies regarding the front of pack labeling.
Politics by Automatic Means? A Critique of Artificial Intelligence Ethics at Work
Authors: Cole Matthew , Cant Callum , Ustek Spilda Funda , Graham Mark
Published: July 2022

Calls for “ethical Artificial Intelligence” are legion, with a recent proliferation of government and industry guidelines attempting to establish ethical rules and boundaries for this new technology. With few exceptions, they interpret Artificial Intelligence (AI) ethics narrowly in a liberal political framework of privacy concerns, transparency, governance and non-discrimination. One of the main hurdles to establishing “ethical AI” remains how to operationalize high-level principles such that they translate to technology design, development and use in the labor process. This is because organizations can end up interpreting ethics in an ad-hoc way with no oversight, treating ethics as simply another technological problem with technological solutions, and regulations have been largely detached from the issues AI presents for workers. There is a distinct lack of supra-national standards for fair, decent, or just AI in contexts where people depend on and work in tandem with it. Topics such as discrimination and bias in job allocation, surveillance and control in the labor process, and quantification of work have received significant attention, yet questions around AI and job quality and working conditions have not. This has left workers exposed to potential risks and harms of AI. In this paper, we provide a critique of relevant academic literature and policies related to AI ethics. We then identify a set of principles that could facilitate fairer working conditions with AI. As part of a broader research initiative with the Global Partnership on Artificial Intelligence, we propose a set of accountability mechanisms to ensure AI systems foster fairer working conditions. Such processes are aimed at reshaping the social impact of technology from the point of inception to set a research agenda for the future. As such, the key contribution of the paper is how to bridge from abstract ethical principles to operationalizable processes in the vast field of AI and new technology at work.
Geo-Environmental Spatial Imaginaries: Reframing Nature Using Soft Spaces and Hybrid Rationalities
Authors: Tippett Joanne , Deas Iain , Haughton Graham
Published: July 2022

This article introduces the idea of hybrid rationalities as a complement to and extension of existing scholarship on hybrid governance and hybrid infrastructure. The research presented here also contributes to work on soft spaces and spatial imaginaries, which has mainly focused on planning and regeneration, by extending consideration to geo-environmental imaginaries and environmental soft spaces. A case study of the Mersey Belt region, which stretches between Manchester and Liverpool in England, reveals the ways in which multiple forms of new rationalities have been absorbed into the work of those looking to promote strategic environmental thinking that works at landscape scale, that is above the level of the individual site. In the process, multiple new geo-environmental spatial imaginaries have been created as part of the process of attracting funders and stakeholders. These new spatial imaginaries have been accompanied by experiments in creating new environmental soft spaces, supported by increasingly hybridized forms of governance in which the roles and rationalities of different stakeholders have to some extent blurred. In the process, actors have shaped, and shared distinctive understandings of how projects to support nature can be used to support wider goals such as addressing climate change, economic regeneration and social well-being.
What Are Medusozoans, and Why Do They Sting?
Authors: Agüero José , Estrada-González Mariae C. , Rosales-Catalán Leonela , Molina-Alonso Ariadne , Mendoza-Becerril María A.
Published: July 2022

Medusozoans are marine animals without backbones that live near the coasts, or in the depths of the oceans. Some of them are large, but others are even tinier than a seed. Medusozoans have been living on Earth for more than 600 million years. Their bodies are 95% water. Some medusozoans live attached to a surface, but others can swim freely. All medusozoans have tentacles, armed with stinging cells called nematocysts. When the nematocysts are triggered, the tiny stingers inside them can travel at speeds up to 67 km per hour. Around 3% of medusozoans are harmful to humans. Therefore, when visiting beaches where these harmful medusozoans live, it is necessary to take some precautions. Wearing protective clothing and not touching a medusozoan stranded on the beach will help you stay safe and healthy, so you can enjoy your beach day!
Expression of CD44, Transforming Growth Factor-β, and Matrix Metalloproteinases in Women With Pelvic Organ Prolapse
Authors: Ying Weiwei , Hu Yanping , Zhu Haibin
Published: July 2022

Background

Defects in the pelvic floor connective tissue may underlie the etiology of pelvic organ prolapse (POP). We hypothesized that the expression of proteins regulating extracellular matrix turnover is altered in the uterosacral ligament of women with POP. We compared the expression of CD44, transforming growth factor (TGF)-β, and matrix metalloproteinases (MMPs) 2/9 in women with and without POP.

Methods and Results

This matched case-control study included 30 postmenopausal women, with POP stage 2 and higher according to the POP quantification system, and 30 postmenopausal women without POP. Immunohistochemical analyses of the uterosacral ligament specimens obtained after hysterectomy were performed to determine CD44, TGF-β, MMP-2, and MMP-9 expression. The expression was quantified using ImageJ software, and the association between prolapse occurrence and risk factors was evaluated using Spearman's correlation analysis. CD44 expressions were significantly lower (p < 0.05), whereas MMP-2 and MMP-9 expression was higher (p < 0.0001 and p < 0.05, respectively), in the POP group than in the control group. The expression of TGF-β was similar in both groups. The occurrence of uterine prolapse was positively correlated with age, postmenopausal age, and MMP-2 and MMP-9 expression (p < 0.01) and negatively correlated with CD44 expression (p < 0.05).

Conclusion

CD44, MMP-2, and MMP-9 may play critical roles in the pathogenesis of POP and may be candidate biomarkers of POP progression.