bluuebunny/crossref_metadata_2025 huggingface dataset:
Light-Induced Activation of a Specific Type-5 Metabotropic Glutamate Receptor Antagonist in the Ventrobasal Thalamus Causes Analgesia in a Mouse Model of Breakthrough Cancer Pain
Authors: Notartomaso Serena , Antenucci Nico , Liberatore Francesca , Mascio Giada , Boccadamo Pompili Stefano Vito , Font Joan , Scioli Mariarosaria , Luongo Livio , Arcella Antonietta , Gradini Roberto , Llebaria Amadeu , Nicoletti Ferdinando
Published: July 2022

Breakthrough cancer pain (BTcP) refers to a sudden and transient exacerbation of pain, which develops in patients treated with opioid analgesics. Fast-onset analgesia is required for the treatment of BTcP. Light-activated drugs offer a novel potential strategy for the rapid control of pain without the typical adverse effects of systemic analgesic drugs. mGlu5 metabotropic glutamate receptor antagonists display potent analgesic activity, and light-induced activation of one of these compounds (JF-NP-26) in the thalamus was found to induce analgesia in models of inflammatory and neuropathic pain. We used an established mouse model of BTcP based on the injection of cancer cells into the femur, followed, 16 days later, by systemic administration of morphine. BTcP was induced by injection of endothelin-1 (ET-1) into the tumor, 20 min after morphine administration. Mice were implanted with optic fibers delivering light in the visible spectrum (405 nm) in the thalamus or prelimbic cortex to locally activate systemically injected JF-NP-26. Light delivery in the thalamus caused rapid and substantial analgesia, and this effect was specific because light delivery in the prelimbic cortex did not relieve BTcP. This finding lays the groundwork for the use of optopharmacology in the treatment of BTcP.
Digital Cognitive Biomarker for Mild Cognitive Impairments and Dementia: A Systematic Review
Authors: Ding Zihan , Lee Tsz-lok , Chan Agnes S.
Published: July 2022

The dementia population is increasing as the world’s population is growing older. The current systematic review aims to identify digital cognitive biomarkers from computerized tests for detecting dementia and its risk state of mild cognitive impairment (MCI), and to evaluate the diagnostic performance of digital cognitive biomarkers. A literature search was performed in three databases, and supplemented by a Google search for names of previously identified computerized tests. Computerized tests were categorized into five types, including memory tests, test batteries, other single/multiple cognitive tests, handwriting/drawing tests, and daily living tasks and serious games. Results showed that 78 studies were eligible. Around 90% of the included studies were rated as high quality based on the Newcastle–Ottawa Scale (NOS). Most of the digital cognitive biomarkers achieved comparable or even better diagnostic performance than traditional paper-and-pencil tests. Moderate to large group differences were consistently observed in cognitive outcomes related to memory and executive functions, as well as some novel outcomes measured by handwriting/drawing tests, daily living tasks, and serious games. These outcomes have the potential to be sensitive digital cognitive biomarkers for MCI and dementia. Therefore, digital cognitive biomarkers can be a sensitive and promising clinical tool for detecting MCI and dementia.
Simultaneous Presbyopia and Astigmatism Correction with a Novel Trifocal Toric Intraocular Lens—A One-Year Follow-Up
Authors: Nováček Ladislav Viktor , Němcová Marie , Sičová Kristýna , Tyx Kateřina , Rozsíval Pavel , Němčanský Jan , Studený Pavel
Published: July 2022

The current investigation evaluates the efficiency of the trifocal toric Liberty 677MTY intraocular lens (IOL) in correcting preoperative corneal astigmatism in cataract patients demanding spectacle independence. The retrospective evaluation included 28 eyes of 15 patients with preoperative corneal astigmatism of at least 1.0 Dioptre (D). All patients were followed up for one year postoperatively. Residual refractive errors and visual acuities at multiple distances were measured. Binocular visual acuity and contrast sensitivity defocus curves were plotted. Visual functions and patient satisfaction were assessed. The efficiency of astigmatism correction was determined using the vector analysis method. The mean spherical equivalent refraction (SEQ) improved from 2.72 ± 1.62 D to 0.10 ± 0.48 D. The cylindric refraction decreased from 1.18 ± 0.45 D to 0.16 ± 0.31 D. Vector analysis proved efficient astigmatism correction with a centroid of 0.10 ± 0.34 D at 161°. Ninety-two percent of eyes resulted within 0.5 D from the target refraction. Visual acuities were 0.1 logMAR or better from +1.0 to −3.5 D defocus values. Visual tasks could be performed without major difficulties. Our patients were highly satisfied. Refractive and visual outcomes with the investigated presbyopia-correcting toric IOL are predictable and the lens provides excellent trifocal vision.
More than Antibiotics: Latest Therapeutics in the Treatment and Prevention of Ocular Surface Infections
Authors: Chiang Ming-Cheng , Chern Edward
Published: July 2022

Ocular surface infections have been common issues for ophthalmologists for decades. Traditional strategies for infection include antibiotics, antiviral agents, and steroids. However, multiple drug-resistant bacteria have become more common with the prevalence of antibiotic use. Furthermore, an ideal treatment for an infectious disease should not only emphasize eliminating the microorganism but also maintaining clear and satisfying visual acuity. Immunogenetic inflammation, tissue fibrosis, and corneal scarring pose serious threats to vision, and they are not attenuated or prevented by traditional antimicrobial therapeutics. Herein, we collected information about current management techniques including stem-cell therapy, probiotics, and gene therapy as well as preventive strategies related to Toll-like receptors. Finally, we will introduce the latest research findings in ocular drug-delivery systems, which may enhance the bioavailability and efficiency of ocular therapeutics. The clinical application of improved delivery systems and novel therapeutics may support people suffering from ocular surface infections.
Relationship between COVID-19 Mortality, Hospital Beds, and Primary Care by Italian Regions: A Lesson for the Future
Authors: Ferrara Nicola , Campobasso Carlo Pietro , Cocozza Sergio , Conti Valeria , Davinelli Sergio , Costantino Maria , Cannavo Alessandro , Rengo Giuseppe , Filippelli Amelia , Corbi Graziamaria
Published: July 2022

One of the characteristics of the SARS-CoV-2 infection in Italy is the significant regional difference in terms of lethality and mortality. These geographical variances were clear in the first wave and confirmed in the second one as well. The study aimed to analyze the correlation between regional differences in COVID-19 mortality and different regional care models, by retrospectively analyzing the association between the Italian COVID-19 deaths and the number of hospital beds, long-term care facilities, general practitioners (GPs), and the health expenditure per capita. The period considered was from 1 March 2020 to 1 March 2021. The number of hospital beds (p < 0.0001) and the number of GPs (p = 0.0094) significantly predicted the COVID-19 death rate. The Italian regions with a higher number of hospital beds and a lower number of GPs showed a higher number of deaths. Multivariate analyses confirmed the results. The Italian regions with a higher amount of centralized healthcare, as represented by the number of hospital beds, experienced a higher number of deaths, while the regions with greater community support, as exemplified by the number of the GPs, faced higher survival. These results suggest the need for a change in the current healthcare system organization.
Special Issue “Multimodality Imaging in Cardiomyopathies”
Authors: D’Andrea Antonello , Bossone Eduardo , Palermi Stefano
Published: July 2022

Multimodality imaging has a crucial role in the identification and management of patients with suspected cardiomyopathies [...]
Comparison of Hospital Readmission and Mortality between COVID-19 and Pneumonia Patients
Authors: Alyabsi Mesnad , Aldibasi Omar , Bosaeed Mohammad , Alanazi Maha , Alqarni Anwar , Albdah Bayan , Alharbi Naif Khalaf , Alghnam Suliman
Published: July 2022

Coronavirus disease 2019 (COVID-19) survivors can have lasting signs and symptoms, including various organ damage, indicating that COVID-19 can be a chronic illness. The current study aims to compare the 30-day hospital readmission and death rate of patients admitted to the hospital with COVID-19 and pneumonia due to other causes. A retrospective cohort study was conducted using data from the Saudi National Guard Health Affairs (NGHA). Records of patients admitted with COVID-19 between 1 March 202 and 31 December 2020 (n = 3597) and pneumonia during 2017 and 2019 (n = 6324) were retrieved and analyzed. We compared the likelihood of 30-day hospital readmission, intensive care unit (ICU) admission, and death between the two groups. Compared with the control group, COVID-19 patients had higher odds of 30-day readmission (odds ratio 1.90, 95% confidence interval 1.61–2.24), higher risk of ICU transfer (hazard ratio 1.85, 95% confidence interval 1.65–2.07), more extended hospital stay (7 vs. 4 days), but less risk of death (hazard ratio 0.18, 95% confidence interval 0.14–0.24). The findings that hospital readmission was higher in COVID-19 recovered patients than in other pneumonia patients inform the current discussion about readmission and death in COVID-19 patients.
Real-World Evidence of the Effectiveness and Safety of Ustekinumab for the Treatment of Crohn’s Disease: Systematic Review and Meta-Analysis of Observational Studies
Authors: Rubín de Célix Cristina , Chaparro María , Gisbert Javier P.
Published: July 2022

(1) Background: Evidence on the outcomes of ustekinumab treatment in real-world Crohn’s disease (CD) patients is needed. Our aim was to evaluate the effectiveness and safety of ustekinumab in CD, reported by observational studies. (2) Methods: bibliographical searches were performed (PubMed, EMBASE). Selection: observational studies assessing the effectiveness and safety of ustekinumab in CD. Exclusion criteria: studies using ustekinumab as a prophylaxis for postoperative recurrence or perianal disease. Data synthesis: effectiveness by intention-to-treat (random-effects model). Data were stratified by study design, population included, administered dose, and prior biologic exposure. (3) Results: A total of 63 studies (8529 patients) were included. Response was achieved in 60% (95% CI, 54–67%) in the short term (8–14 weeks); 64% (57–71%) in the medium term (16–24 weeks); and 64% (52–74%) in the long term (48–52 weeks). Remission was achieved in 37% (28–46%) in the short term; 42% (36–49%) in the medium term; and 45% (37–53%) in the long term. The endoscopic remission rate was 33% (25–40%) in the long term. Eighteen percent of patients lost response during follow-up. Nearly one-third of the patients needed dose optimisation, and in 59% of them it was effective. Twenty-five percent of patients developed adverse events, leading to treatment withdrawal in seven percent of the cases. (4) Conclusions: Ustekinumab is an effective and safe therapy in real-world refractory CD patients. Dose optimisation is frequently required, being effective in a high percentage of cases.
New Strategies to Improve the Quality of Life for Normal Aging versus Pathological Aging
Authors: Bacanoiu Manuela Violeta , Danoiu Mircea
Published: July 2022

In the context of the manifestation of the phenomenon of normal aging and functional decline at older adults with neurodegenerative pathology, the development of physical activities and healthy lifestyle has become a priority that involves many decisions and responsibilities. Therefore, the study of the quality of life of the elderly in terms of delaying early aging and improving the lifestyle of patients with neurodegenerative diseases is a scientific challenge representing research of great interest and relevance. By promoting physical activity based on telerehabilitation programs or performed according to coordinated training either in the community or at home for both study groups, significant improvements have been obtained. The aim of this paper was to evaluate the intervention suitable patterns, surveys delivered through variables online platforms and tools to reflect the stagnation of early aging and the evolution of patients with PD and dementia. Our study involved selected original studies, intensively processed, which demonstrated through intervention specific tools, such as quantitative, qualitative, socio-economics, physical, and cognitive indicators, that significant improvements can be achieved in the process of early aging, but also significant progress in patients with neurodegenerative diseases. By searching the last five years of papers, our review, presents the importance of intervention by telerehabilitation or by scheduled physical exercises quantified by specific indicators.
Fatal Acute Heart Failure in the Course of Macrophage Activation Syndrome: Case Report and Literature Review
Authors: Kuna Jakub , Chmielewski Grzegorz , Gruchała Marcin , Szade Jolanta , Mikiewicz Mateusz , Ręcki Przemysław , Krajewska-Włodarczyk Magdalena
Published: July 2022

Macrophage activation syndrome is a severe and potentially fatal condition in rheumatology. It can involve many different organs and systems, including the cardiovascular system, but heart failure due to its course is a relatively rare occurrence. In the following paper, we present a case of a young woman with newly diagnosed systemic lupus erythematosus who, in the span of two months, developed macrophage activation syndrome and acute heart failure, which caused her death. We analyze potential causes that may have led to that outcome, and present a brief review of the current literature concerning different macrophage groups in the heart and their potential involvement in the development of heart failure.
TNF-α Inhibitors in Combination with MTX Reduce Circulating Levels of Heparan Sulfate/Heparin and Endothelial Dysfunction Biomarkers (sVCAM-1, MCP-1, MMP-9 and ADMA) in Women with Rheumatoid Arthritis
Authors: Szeremeta Anna , Jura-Półtorak Agnieszka , Zoń-Giebel Aleksandra , Olczyk Krystyna , Komosińska-Vassev Katarzyna
Published: July 2022

Sulfated glycosaminoglycans (sGAGs) are likely to play an important role in the development and progression of rheumatoid arthritis (RA)-associated atherosclerosis. The present study investigated the effect of anti-tumor necrosis factor-α (anti-TNF-α) therapy in combination with methotrexate on plasma sGAG levels and serum markers of endothelial dysfunction. Among sGAG types, plasma chondroitin/dermatan sulfate (CS/DS) and heparan sulfate/heparin (HS/H) were characterized using electrophoretic fractionation. Serum levels of soluble vascular cell adhesion molecule-1 (sVCAM-1), monocyte chemoattractant protein-1 (MCP-1), matrix metalloproteinase-9 (MMP-9) and asymmetric dimethylarginine (ADMA) were measured by immunoassays. The measurements were carried out four times: at baseline and after 3, 9 and 15 months of anti-TNF-α therapy. All analyzed parameters, excluding ADMA, were significantly elevated in patients with RA before the implementation of biological therapy compared to healthy subjects. Performed anti-TNF-α treatment led to a successive decrease in HS/H levels toward normal values, without any effect on CS/DS levels in female RA patients. The treatment was also effective at lowering the serum levels of sVCAM-1, MCP-1, MMP-9 and ADMA. Moreover, a significant positive correlation was found between the circulating HS/H and the 28 joint disease activity score based on the erythrocyte sedimentation rate (DAS28-ESR, r = 0.408; p <0.05), MCP-1 (r = 0.398; p <0.05) and ADMA (r = 0.396; p <0.05) in patients before the first dose of TNF-α inhibitor. In conclusion, a beneficial effect of anti-TNF-α therapy on cell-surface heparan sulfate proteoglycans (HSPGs)/HS turnover and endothelial dysfunction was observed in this study. This was manifested by a decrease in blood HS/H levels and markers of endothelial activation, respectively. Moreover, the decrease in the concentration of HS/H in the blood of patients during treatment, progressing with the decline in disease activity, indicates that the plasma HS/H profile may be useful for monitoring the efficacy of anti-TNF-α treatment in patients with RA.
Endovascular Treatment of Intracranial Vein and Venous Sinus Thrombosis—A Systematic Review
Authors: Bücke Philipp , Hellstern Victoria , Cimpoca Alexandru , Cohen José E. , Horvath Thomas , Ganslandt Oliver , Bäzner Hansjörg , Henkes Hans
Published: July 2022

Background: Cerebral venous sinus or vein thromboses (SVT) are treated with heparin followed by oral anticoagulation. Even after receiving the best medical treatment, numerous patients experience neurological deterioration, intracerebral hemorrhage or brain edema. Debate regarding whether endovascular treatment (EVT) is beneficial in such severe cases remains ongoing. This systematic review summarizes the current evidence supporting the use of EVT for SVT on the basis of case presentations, with a focus on patient selection, treatment strategies and the effects of the COVID-19 pandemic. Methods: This systemic literature review included randomized controlled trials (RCTs) and retrospective observational data analyzing five or more patients. Follow-up information (modified Rankin scale (mRS)) was required to be provided (individual patient data). Results: 21 records (n = 405 patients; 1 RCT, 20 observational studies) were identified. EVT was found to be feasible and safe in a highly selected patient cohort but was not associated with an increase in good functional outcomes (mRS 0–2) in RCT data. In observational data, good functional outcomes were frequently observed despite an anticipated poor prognosis. Conclusion: The current evidence does not support the routine incorporation of EVT in SVT treatment. However, in a patient cohort prone to poor prognosis, EVT might be a reasonable therapeutic option. Further studies determining the patients at risk, choice of methods and devices, and timing of treatment initiation are warranted.
Optimization of Friction Stir Welding Parameters in Hybrid Additive Manufacturing: Weldability of 3D-Printed Poly(methyl methacrylate) Plates
Authors: Vidakis Nectarios , Petousis Markos , Mountakis Nikolaos , Kechagias John D.
Published: July 2022

In this work, the expansion of friction stir welding (FSW) in parts made via material extrusion (MEX) 3D printing was investigated. Poly(methyl methacrylate) (PMMA) plates were joined in a full factorial experimental design. The effects of three FSW parameters (weld tool pin geometry, rotating speed, and travel speed) on the weld results were studied. The tensile strength was investigated using statistical modeling tools. A morphological characterization study was also conducted on the weld zone, with microscopy. The state of the material during the FSW process was monitored via real-time temperature measurements. The feasibility of the process was verified. The results show high industrial merit for the process. The highest tensile strength was reported for the sample welded with the frustum tool, at 1400 rpm and a 9 mm/min travel speed (the highest studied), with a welding efficiency > 1. This can be attributed to the reduced porosity of the weld area compared to the 3D printed structure, and indicates a high potential for joining 3D-printed PMMA sheets via the FSW process.
Numerical Study of Wave Drift Load and Turning Characteristics of KVLCC2 Ship in Regular Waves Based on TEBEM
Authors: Chen Ji-Kang , Zhang Guo-Dong , Duan Wen-Yang
Published: July 2022

Maritime traffic has increased considerably in recent years, making energy efficiency and navigation safety of ships more crucial than ever. Hence, a two-time scale model based on the Taylor expansion boundary element method (TEBEM) is proposed to predict ship turning trajectories in regular waves. The maneuvering motion is calculated using a three degrees of freedom MMG model that considers the wave drift loads. TEBEM overcomes the shortcomings of the constant panel method in solving tangential induced velocity at a non-smooth boundary and that of the high-order boundary element method in dealing with a high-order derivative of the velocity potential at the corner. This significantly improves the calculation accuracy of the induced velocity and high-order derivative of velocity potential. Firstly, based on the TEBEM, the surge and sway wave drift forces and yaw moment of the KVLCC2 model with drift angle under full wave headings are calculated and compared with computational fluid dynamics results, using which the calculation accuracy of TEBEM is verified. Subsequently, the two-time scale model is used to calculate the turning trajectories of the KVLCC2 model in regular waves with different wave headings, wave frequencies, and wave steepness. The numerical results show that the drift angle has a certain effect on the wave drift loads of the ship, and the proposed model can effectively predict the ship’s turning motion in regular waves.
Predicting Loss of Efficacy after Non-Medical Switching: Correlation between Circulating TNF-α Levels and SB4 in Etanercept to SB4 Switchers and Naïve Patients with Rheumatic Disease
Authors: Benucci Maurizio , Damiani Arianna , Bandinelli Francesca , Russo Edda , Li Gobbi Francesca , Grossi Valentina , Amedei Amedeo , Infantino Maria , Manfredi Mariangela
Published: July 2022

Background: We investigated how the non-medical switching (NMS) between Etanercept (ETN)/originator and SB4/biosimilar affects treatment efficacy in a rheumatic disease (RD) cohort, evaluating some laboratory parameters as loss of efficacy predictors after NMS. Methods: We enrolled 124 patients with RD (rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis): 79 switchers from ETN/originator to SB4 and 45 naïve patients receiving SB4 (first biological treatment). At baseline, 6 (T1), and 12 months (T2), clinical and laboratory parameters were evaluated. Results: In naïve patients, TNF-α significantly increased at T1 in responders (NR) and non-responders (NNR). TNF-α was lower in NNR than in NR at T1 and T2. In NR and NNR, drug levels (DL) increased between T1 and T2. However, DLs were lower in NNR than in NR at T1 and T2. TNF-α was higher in switcher responders (SR) than in non-responders (SNR) at T1 and T2. In SNR, DLs were higher at baseline than in SR, but they decreased significantly at T1 and T2. Conclusions: We observed a decrease in DL and TNF-α levels after NMS in SNR. Moreover, in naïve patients, DL and TNF-α levels were higher in NR than in NNR. Monitoring DL and TNF-α levels may represent a future precision medicine approach to predict loss of efficacy after NMS.
Cardiovascular Risk Prediction Models and Scores in the Era of Personalized Medicine
Authors: Sofogianni Areti , Stalikas Nikolaos , Antza Christina , Tziomalos Konstantinos
Published: July 2022

Cardiovascular disease (CVD) is the leading cause of death worldwide. Management of cardiovascular risk factors, particularly hypertension and dyslipidemia, has been shown to reduce cardiovascular morbidity and mortality. However, current guidelines recommend adjusting the intensity of blood pressure- and lipid-lowering treatment according to the cardiovascular risk of the patient. Therefore, cardiovascular risk prediction is a sine qua non for optimizing cardiovascular prevention strategies, particularly in patients without established CVD or type 2 diabetes mellitus (T2DM). As a result, several cardiovascular risk prediction equations have been developed. Nevertheless, it is still unclear which is the optimal prediction risk equation. In the present review, we summarize the current knowledge regarding the accuracy of the most widely used cardiovascular risk prediction equations. Notably, most of these risk scores have not been validated in external cohorts or were shown to over- or underestimate risk in populations other than those in which they derive. Accordingly, country-specific risk scores, where available, should be preferred for cardiovascular risk stratification.
The Medical, Clinical, and Radiographic Aspects of Multiple Idiopathic Tooth Resorption: A Systematic Review
Authors: Richert Raphaël , Santamaria Julie , Laforest Laurent , Maurin Jean-Christophe
Published: July 2022

Background: Many causes of resorption remain unclear and are thus identified as idiopathic. In such cases, management is difficult, especially when multiple teeth are involved. The aim of the present study was to assess the literature regarding the medical, clinical, and radiographic aspects of multiple idiopathic resorptions (MIR) and to examine the factors associated with the risk of extraction. Methods: The title and protocol were registered a priori in PROSPERO (CRD42020191564), and the study followed the PRISMA methodology. Four electronic databases were searched to include reviews and case reports on MIR in permanent dentition. Results: Among the 1035 articles identified, 31 case reports were included. The mean age of the patients was 32 years (SD = 16.4). MIR were consistently diagnosed after radiographic evaluation and were undetected during intra-oral examination in 62% of cases. The treatment involved extraction in 77% of cases. The risk of extraction increased in the presence of periodontal inflammation. Conclusions: MIR are aggressive forms of resorption requiring routine visits. MIR mostly involve extraction and lead to a challenging prosthetic rehabilitation due to severely damaged abutment teeth. However, the current knowledge on MIR remains fragmental and based on a limited number of case reports.
The Impact of Vertical Theories of Harm on Investor Returns: An Event Study of US Vertical Mergers
Authors: Sonenshine Ralph , Da Seyni
Published: July 2022

The welfare implications of vertical mergers have been a subject of disagreement for decades. Similar to horizontal mergers, economists need to weigh the efficiency gains relative to the market power concerns when considering the competitive effects of vertical mergers. However, in vertical mergers, regulators are also concerned with other potential harmful effects, such as input and customer foreclosure. Using an event style technique, this paper explores these vertical theories of harm by comparing the abnormal returns of acquirers, targets, and the two combined in vertical and horizontal mergers that were challenged by regulators as potentially anticompetitive. Our results indicate that abnormal returns to targets were similar between vertical and horizontal mergers, but the gains to targets relative to acquirers were far higher in vertical versus horizontal mergers (53.6% versus 39.5%). In addition, we found that exclusionary effects have a positive impact (0.24% of the dollar abnormal return) on the bargaining position of targets. In contrast, acquirers gain 0.45% and 0.39% of the dollar abnormal return relative to targets when the antitrust concern entails collusive effects or elimination of potential competition, respectively.
Landscape Pattern Vulnerability of the Eastern Hengduan Mountains, China and Response to Elevation and Artificial Disturbance
Authors: Sun Jiarui , Zhou Lu , Zong Hua
Published: July 2022

The eastern Hengduan Mountains are located in the transition zone between the Qinghai-Tibet Plateau and the Sichuan Basin and are important for global biodiversity and water conservation in China. However, their landscape pattern vulnerability index (LVI) and its influencing factors have not been systematically studied. Therefore, the spatial distribution patterns, LVI, and the landscape artificial disturbance intensity (LHAI) of Ganzi Prefecture were analyzed using ArcGIS software based on landscape data and Digital Elevation Model (DEM) digital elevation data. Then, the LVI response to LHAI and elevation was discussed. The results showed that Ganzi Prefecture was dominated by low- and middle-LVI areas, together accounting for 56.45% of the total area. LVI values were highest in the northern regions, followed by the southern and eastern regions. Batang and Derong counties had the highest LVI values. Most areas in Ganzi Prefecture had very low- or low-LHAI values, accounting for 81.48% of the total area, whereas high-LHAI areas accounted for 2.32% of the total area. Both the LVI and LHAI of Ganzi Prefecture had clustered distributions. Spearman analysis indicated that when elevation exceeded 4500 m, it was the most important factor affecting LVI and LHAI. In the range of 4500–5400 m, the relationship between elevation and LVI shifted from a weak positive correlation to a negative correlation, whereas LHAI was positively correlated with elevation. In addition, LVI also responded significantly to LHAI. However, the relationship kept changing as elevation increased. Hence, the ecological vulnerability of high elevation areas above 4500 m deserves greater attention. In addition, pasture areas in the upstream reaches of the Yalong River in the northern region, the coastal area in the downstream reaches of the Jinsha River in the southern region, and the eastern mining area, should be prioritized for protection and restoration. This research provides a basis for appropriate environmental planning mechanisms and policy protections at the landscape level.
Contact Characteristics at Interface in Three-Body Contact Conditions with Rough Surfaces and Foreign Particles
Authors: Chern Shin-Yuh , Chen Yang-Yuan , Liu Wei-Lun , Horng Jeng-Haur
Published: July 2022

Nanoparticles as lubricant additives under a certain average diameter and concentration may reduce wear, friction and scuffing damage. However, atmospheric dust particles affect not only human health but also the efficiency of components, and even cause component failures. Therefore, the contact characteristics at interfaces with foreign particles require careful investigation. In this work, a 3-body microcontact mechanics concept is used to analyze the effects of wear debris and foreign particles on real contact area, contact mode, asperity deformation type and separation at interface. The results show that the relationship profile between dimensionless real contact area (At*) and dimensionless normal contact load (Ft*) is wedge-shaped in a 3-body contact interface. Using surface-to-surface 2-body contact area as upper bound and surface-to-particle 3-body contact as lower bound, the 3-body hybrid contact situation is in between upper and lower bounds. As the dimensionless normal contact load increases, At* increases gradually as well. The order of contact mode is p-s contact, hybrid contact and then s-s contact. If the 3-body contact interface is in hybrid contact mode, the decrease in the hardness and average third body diameter will cause the At* to increase significantly at the same Ft*. Conversely, the separation and real contact area ratio of plastic deformation decrease gradually. The turning point of contact area (TPCA) occurs when the contact mode is within hybrid contact mode and the ratio of average third body diameter to the composite equivalent surface RMS roughness is about 50–70% for foreign particles and wear debris. When the Ft* is slightly larger than Ftpca*, the third body and surface share the total interface load approximately equally which will help reduce the real contact pressure and plastic contact area to improve surface performance.
Shear Bond Strength of a Direct Resin Composite to CAD-CAM Composite Blocks: Relative Contribution of Micromechanical and Chemical Block Surface Treatment
Authors: Fouquet Vincent , Lachard François , Abdel-Gawad Sarah , Dursun Elisabeth , Attal Jean-Pierre , François Philippe
Published: July 2022

This study aims to compare the shear bond strength (SBS) of a direct resin composite to CAD-CAM resin composite blocks treated with different surface treatments: micromechanical, chemical or a combination of both. Eight CAD-CAM resin composite blocks, namely Brilliant Crios, Cerasmart 270, Vita Enamic, Grandio block, Katana Avencia, Lava Ultimate, Tetric CAD and Shofu Block HC were chosen. The micromechanical surface treatment protocols tested were hydrofluoric acid, polyacrylic acid or sandblasting, and the chemical one was a universal primer. These treated CAD-CAM blocks were tested to determine the SBS of a light-curing composite resin Z100 bonded to their surface. Two-way ANOVA followed by Tukey’s post hoc test was used to investigate the difference in SBS. Failures were analyzed by Fisher’s exact test. Bonding interfaces were examined by scanning electron microscopy. The micromechanical surface treatments give the highest SBS values: sandblasting appears to be the most efficient procedure for dispersed filler composite blocks, while hydrofluoric acid etching is preferable for polymer-infiltrated ceramic network (PICN) blocks. The use of universal primer does not improve SBS values on dispersed filler composite blocks. For PICN blocks, the use of universal primer significantly increases SBS values when combined with hydrofluoric acid etching.
Cu and As(V) Adsorption and Desorption on/from Different Soils and Bio-Adsorbents
Authors: Cela-Dablanca Raquel , Barreiro Ana , Ferreira-Coelho Gustavo , Campillo-Cora Claudia , Pérez-Rodríguez Paula , Arias-Estévez Manuel , Núñez-Delgado Avelino , Álvarez-Rodríguez Esperanza , Fernández-Sanjurjo María J.
Published: July 2022

This research is concerned with the adsorption and desorption of Cu and As(V) on/from different soils and by-products. Both contaminants may reach soils by the spreading of manure/slurries, wastewater, sewage sludge, or pesticides, and also due to pollution caused by mining and industrial activities. Different crop soils were sampled in A Limia (AL) and Sarria (S) (Galicia, NW Spain). Three low-cost by-products were selected to evaluate their bio-adsorbent potential: pine bark, oak ash, and mussel shell. The adsorption/desorption studies were carried out by means of batch-type experiments, adding increasing and individual concentrations of Cu and As(V). The fit of the adsorption data to the Langmuir, Freundlich, and Temkin models was assessed, with good results in some cases, but with high estimation errors in others. Cu retention was higher in soils with high organic matter and/or pH, reaching almost 100%, while the desorption was less than 15%. The As(V) adsorption percentage clearly decreased for higher As doses, especially in S soils, from 60–100% to 10–40%. The As(V) desorption was closely related to soil acidity, being higher for soils with higher pH values (S soils), in which up to 66% of the As(V) previously adsorbed can be desorbed. The three by-products showed high Cu adsorption, especially oak ash, which adsorbed all the Cu added in a rather irreversible manner. Oak ash also adsorbed a high amount of As(V) (>80%) in a rather non-reversible way, while mussel shell adsorbed between 7 and 33% of the added As(V), and pine bark adsorbed less than 12%, with both by-products reaching 35% desorption. Based on the adsorption and desorption data, oak ash performed as an excellent adsorbent for both Cu and As(V), a fact favored by its high pH and the presence of non-crystalline minerals and different oxides and carbonates. Overall, the results of this research can be relevant when designing strategies to prevent Cu and As(V) pollution affecting soils, waterbodies, and plants, and therefore have repercussions on public health and the environment.
The Antibacterial and Cytotoxic Effects of Silver Nanoparticles Coated Titanium Implants: A Narrative Review
Authors: Haugen Håvard J. , Makhtari Soukayna , Ahmadi Sara , Hussain Badra
Published: July 2022

Nanotechnology has become an emerging research field with numerous biomedical scientific applications. Silver possesses bactericidal activities that have been harnessed for centuries; however, there is a concern about the toxic effects of silver nanoparticles. This paper aims to provide an overview of silver-treated dental implants and discuss their potential to reduce the prevalence of peri-implant diseases. An electronic search was performed using PubMed. After screening, data extraction was performed on the 45 remaining articles using inclusion and exclusion criteria. Most of the articles demonstrated that silver nanoparticles embedded in a coating layer and/or on surface-treated titanium exhibit sound antibacterial effects and biocompatibility. Most of the reviewed studies revealed that silver nanoparticles on dental implant surfaces reduced cytotoxicity but provided a prolonged antibacterial effect. The cytotoxicity and antibacterial effect are closely linked to how the silver nanoparticles are released from the titanium surfaces, where a slower release increases cell viability and proliferation. However, to improve the clinical translation, there is still a need for more studies, especially evaluating the long-term systemic effects and studies recreating the conditions in the oral cavity.
Mesenchymal Stem Cell-Derived Extracellular Vesicles for Bone Defect Repair
Authors: Wang Dongxue , Cao Hong , Hua Weizhong , Gao Lu , Yuan Yu , Zhou Xuchang , Zeng Zhipeng
Published: July 2022

The repair of critical bone defects is a hotspot of orthopedic research. With the development of bone tissue engineering (BTE), there is increasing evidence showing that the combined application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) (MSC-EVs), especially exosomes, with hydrogels, scaffolds, and other bioactive materials has made great progress, exhibiting a good potential for bone regeneration. Recent studies have found that miRNAs, proteins, and other cargo loaded in EVs are key factors in promoting osteogenesis and angiogenesis. In BTE, the expression profile of the intrinsic cargo of EVs can be changed by modifying the gene expression of MSCs to obtain EVs with enhanced osteogenic activity and ultimately enhance the osteoinductive ability of bone graft materials. However, the current research on MSC-EVs for repairing bone defects is still in its infancy, and the underlying mechanism remains unclear. Therefore, in this review, the effect of bioactive materials such as hydrogels and scaffolds combined with MSC-EVs in repairing bone defects is summarized, and the mechanism of MSC-EVs promoting bone defect repair by delivering active molecules such as internal miRNAs is further elucidated, which provides a theoretical basis and reference for the clinical application of MSC-EVs in repairing bone defects.
Phase-Field Simulation of Spinodal Decomposition in Mn-Cu Alloys
Authors: Sigala-García Darío A. , López-Hirata Víctor M. , Saucedo-Muñoz Maribel L. , Dorantes-Rosales Héctor J. , Villegas-Cárdenas José D.
Published: July 2022

The spinodal decomposition was studied in the aged Mn-40 at. %Cu, Mn-30 at. %Cu, Mn-20 at. %Cu alloys using a phase-field model based on the Cahn–Hillard equation, considering a subregular solution model and the energy contribution of the magnetic behavior. The simulations were performed at aging temperatures of 300, 400, and 500 °C for times from 1 to 240 min. The growth kinetics of the Mn concentration profiles with time indicated clearly that the phase decomposition of the supersaturated solid solution γ into a mixture of Mn-rich γ′ and Cu-rich γ phases occurred by the spinodal decomposition mechanism. Moreover, the phase decomposition at the early stages of aging exhibited the characteristic morphology of spinodal decomposition, an interconnected and percolated microstructure of the decomposed phases. The most rapid growth kinetics of spinodal decomposition occurred for the aging of Mn-20 and 30 at. %Cu alloys because of the higher driving force. The presence of the phase decomposition is responsible for the increase in hardness, as well as the improvement of the damping capacity of Mn-Cu alloys.
In-Production Rheometry of Semi-Solid Metal Slurries
Authors: Jarfors Anders E. W. , Jafari Mahdi , Aqeel Muhammed , Liljeqvist Patrik , Jansson Per
Published: July 2022

Semi-solid aluminium alloy processing (SSM) has advanced into a more mature process with many applications. The current paper aims to investigate the in-process behaviour of a production slurry using an engineering approach to estimate the properties. A method to assess the rheological properties of a semi-solid metal slurry was tested and found capable of producing meaningful measurements. The foundations of this were to use a Rushton turbine setup for the assessment through a model to optimize the factors included in the slurry rheology. In the analysis of the static and dynamic part of the viscosity, it was concluded that the slurry turns increasingly into a solid with increasing solid fractions where the static shear strength dominates the rheology more and more compared to the dynamic components. The static yield strength was also found significantly more dominant with increasing solid fraction, suggesting that the industrial-scale analysis using models based on Ostwald–de Waale and Carreau modelling is far from sufficient for a process, such as the RheoMetal process, with a very rapid slurry generation.
Molecular Network-Based Identification of Tramadol Metabolites in a Fatal Tramadol Poisoning
Authors: Magny Romain , Auzeil Nicolas , Lefrère Bertrand , Mégarbane Bruno , Houzé Pascal , Labat Laurence
Published: July 2022

Identification of xenobiotics and their phase I/II metabolites in poisoned patients remains challenging. Systematic approaches using bioinformatic tools are needed to detect all compounds as exhaustively as possible. Here, we aimed to assess an analytical workflow using liquid chromatography coupled to high-resolution mass spectrometry with data processing based on a molecular network to identify tramadol metabolites in urine and plasma in poisoned patients. The generated molecular network from liquid chromatography coupled to high-resolution tandem mass spectrometry data acquired in both positive and negative ion modes allowed for the identification of 25 tramadol metabolites in urine and plasma, including four methylated metabolites that have not been previously reported in humans or in vitro models. While positive ion mode is reliable for generating a network of tramadol metabolites displaying a dimethylamino radical in their structure, negative ion mode was useful to cluster phase II metabolites. In conclusion, the combined use of molecular networks in positive and negative ion modes is a suitable and robust tool to identify a broad range of metabolites in poisoned patients, as shown in a fatal tramadol-poisoned patient.
Special Issue: Type III Secretion Systems in Human/Animal Pathogenic Bacteria
Authors: Bernal-Bayard Joaquín , Ramos-Morales Francisco
Published: July 2022

Type III secretion systems (T3SSs) are molecular devices that are essential for the communication of many Gram-negative bacteria with their eukaryotic hosts [...]
Characteristics and Differences Analysis for Thermal Evolution of Wufeng–Longmaxi Shale, Southern Sichuan Basin, SW China
Authors: Lyu Peixi , Meng Jianghui , Pan Renfang , Yi Xuefei , Yue Tao , Zhang Ning
Published: July 2022

The marine shale of the Upper Ordovician Wufeng Formation–Lower Silurian Longmaxi Formation is the main source rock and the target of shale gas exploration in the southern Sichuan Basin. The maturity of organic matter (OM) is a vital indicator for source rock evaluation. Due to the lack of vitrinite, the organic matter maturity of the Wufeng–Longmaxi Formations in the southern Sichuan Basin is difficult to accurately evaluate. In total, 33 core samples of the Wufeng–Longmaxi Formations in the southern Sichuan Basin were selected to observe the optical characteristics of solid bitumen and graptolites and measure their random reflectance. Simultaneously, Raman spectroscopic parameters of kerogen were also used to quantitatively analyze the change in maturity. By using Raman spectroscopic parameters as mediators, conversion equations between graptolite random reflectance (GRor) and equivalent vitrinite reflectance (EqVRo) were established. Taking the calculation results of EqVRo as constraints, the tectono-thermal evolution history of Wufeng–Longmaxi Shale in the southern Sichuan Basin is constructed through basin modelling. The results show that the maturity of Wufeng–Longmaxi Shale in the western Changning, Luzhou-western Chongqing, eastern Changning and Weiyuan areas decreases successively. The EqVRo falls in the ranges of 3.61%~3.91%, 2.92%~3.57%, 3.08%~3.25%, 2.41%~3.12%, and the average EqVRo is 3.73%, 3.30%, 3.18% and 2.80%, respectively. Thermal evolution in western Changning was controlled by the thermal effect of the Emeishan mantle plume and paleo-burial depth, while the thermal evolution of other areas was mainly controlled by paleo-burial depth. This study provides a reliable parameter for the evaluation of thermal maturity and makes a more accurate calibration of the maturity of the Wufeng–Longmaxi Formations in the southern Sichuan Basin; it also expounds the factors for the differences in thermal evolution in different parts of the area.
Air-Depleted and Solvent-Impregnated Cork Powder as a New Natural and Sustainable Fining Agent for Removal of 2,4,6-Trichloroanisole (TCA) from Red Wines
Authors: Cosme Fernanda , Gomes Sara , Vilela Alice , Filipe-Ribeiro Luís , Nunes Fernando M.
Published: July 2022

Trichloroanisole (TCA) in wine results in a sensory defect called “cork taint”, a significant problem for the wine industry. Wines can become contaminated by TCA absorption from the atmosphere through contaminated wood barrels, cork stoppers, and wood pallets. Air-depleted solvent-impregnated (ADSI) cork powder (CP) was used to mitigate TCA in wines. The ADSI CP (0.25 g/L) removed 91% of TCA (6 ng/L levels), resulting in an olfactory activity value of 0.14. A Freundlich isotherm described ADSI CP TCA adsorption with irreversible adsorption and a KF = 33.37. ADSI CP application had no significant impact on the phenolic profile and chromatic characteristics of red wine. Using headspace sampling with re-equilibration, an average reduction in the volatile abundance of 29 ± 15%, 31 ± 19%, and 37 ± 24% was observed for the 0.10, 0.25, and 0.50 g/L ADSI CP, respectively. The alkyl esters and acids were the most affected. The impact observed was much lower when using headspace sampling without re-equilibration. Isoamyl acetate, ethyl hexanoate, ethyl hexanoate, and ethyl decanoate abundances were not significantly different from the control wine and 0.25 g/L ADSI CP application. Thus, ADSI CP can be a new sustainable fining agent to remove this “off-flavor” from wine, with a reduced impact on the wine characteristics.
Influence of the Surface Chemical Composition Differences between Zirconia and Titanium with the Similar Surface Structure and Roughness on Bone Formation
Authors: Tokunaga Yoshiki , Hirota Masatsugu , Hayakawa Tohru
Published: July 2022

The osseointegration of zirconia (ZrO2) implants is still controversial. In this study, we aimed to make clear the influence of surface chemical composition, Ti or ZrO2, to osseointegration. First, a roughened Ti surface was prepared with a combination of large-grit sandblasting and acid treatment. Then, we applied molecular precursor solution containing Zr complex onto roughened Ti surface and can deposit thin ZrO2 film onto roughened Ti surface. We can change surface chemical composition from Ti to ZrO2 without changing the surface structure and roughness of roughened Ti. The tetragonal Zr was uniformly present on the ZrO2-coated Ti surface, and the surface of the ZrO2-coated Ti showed a higher apparent zeta potential than Ti. Ti and ZrO2-coated Ti rectangular plate implant was placed into the femur bone defect. After 2 and 4 weeks of implantation, histomorphometric observation revealed that the bone-to-implant contact ratio and the bone mass values for ZrO2-coated Ti implants inserted into the femur bone defects of the rats at 2 weeks were significantly higher than those for Ti implants (p < 0.05). It revealed that ZrO2 with a similar surface structure and roughness as that of roughened Ti promoted osteogenesis equivalent to or better than that of Ti in the early bone formation stage.