bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of the Role of Dinutuximab Beta-Based Immunotherapy in the SIOPEN High-Risk Neuroblastoma 1 Trial (HR-NBL1)
Authors: Ladenstein Ruth , Pötschger Ulrike , Valteau-Couanet Dominique , Luksch Roberto , Castel Victoria , Ash Shifra , Laureys Genevieve , Brock Penelope , Michon Jean Marie , Owens Cormac , Trahair Toby , Chi Fung Chan Godfrey , Ruud Ellen , Schroeder Henrik , Beck-Popovic Maja , Schreier Guenter , Loibner Hans , Ambros Peter , Holmes Keith , Castellani Maria Rita , Gaze Mark N. , Garaventa Alberto , Pearson Andrew D.J. , Lode Holger N.
Published: January 2020

To explore the effects of immunotherapy in the International Society of Paediatric Oncology Europe Neuroblastoma Group SIOPEN high-risk neuroblastoma 1 trial (HR-NBL1 trial), two cohorts were studied: one prior to and one after the introduction of dinutuximab beta. All patients received standard induction and high-dose therapy (HDT) with autologous stem cell rescue (ASCR); the local control comprised surgery and radiotherapy to the primary tumour site, followed by isotretinoin. A landmark timepoint of 109 days, resulting from the median time between ASCR and initiation of immunotherapy, was used to define patients’ eligibility in the pre-immunotherapy analysis cohort. Median follow-up was 5.8 years (inter-quartile range (IQR): 4.2–8.2 years) for 844 eligible patients balanced for risk factors, such as age, sex, stage 4, MYCN amplification and response prior to HDT. The five-year event-free and overall survival (95% confidence interval (CI) of 466 patients not receiving immunotherapy was 42% (38–47%) and 50% (46–55%) but was 57% (51–62%) and 64% (59–69%) for 378 patients receiving immunotherapy (p < 0.001). A multivariate analysis identified absence of immunotherapy (p = 0.0002, hazard ratio (HR) 1.573); type of HDT (p = 0.0029, HR 1.431); less than complete response prior to maintenance therapy (p = 0.0043, HR 1.494) and >1 metastatic compartment at diagnosis (p < 0.001, HR 2.665) as risk factors for relapse or progression. Results suggest an important role for dinutuximab beta-based immunotherapy within the treatment concepts applied in HR-NBL1/SIOPEN.
Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine
Authors: Zhang Weiyi , Gao Jie , Cheng Chuanjing , Zhang Man , Liu Wenjuan , Ma Xiaoyao , Lei Wei , Hao Erwei , Hou Xiaotao , Hou Yuanyuan , Bai Gang
Published: January 2020

At present, melanoma is a common malignant tumor with the highest mortality rate of all types of skin cancer. Although the first option for treating melanoma is with chemicals, the effects are unsatisfactory and include poor medication response and high resistance. Therefore, developing new medicines or a novel combination approach would be a significant breakthrough. Here, we present cinnamaldehyde (CA) as a potential candidate, which exerted an antitumor effect in melanoma cell lines. Chemical biology methods of target fishing, molecular imaging, and live cell tracing by an alkynyl–CA probe revealed that the α-enolase (ENO1) protein was the target of CA. The covalent binding of CA with ENO1 changed the stability of the ENO1 protein and affected the glycolytic activity. Furthermore, our results demonstrated that dacarbazine (DTIC) showed a high promoting effect with CA for antimelanoma both in vivo and in vitro. The combination improved the DTIC cell cycle arrest in the S phase and markedly impacted melanoma growth. As a covalent inhibitor of ENO1, CA combined with DTIC may be beneficial in patients with drug resistance in antimelanoma therapy.
Simultaneous Immobilization of Soil Cd(II) and As(V) by Fe-Modified Biochar
Authors: Wang Yi-min , Wang Shao-wei , Wang Cheng-qian , Zhang Zhi-yuan , Zhang Jia-qi , Meng Meng , Li Ming , Uchimiya Minori , Yuan Xu-yin
Published: January 2020

Remediation of soil heavy metal by biochar has been extensively studied. However, few studies focused on the role of biochar on the co-immobilization of cadmium (Cd(II)) and arsenate (As(V)) and related soil nutrient availability. Remediation tests were conducted with three types of pristine and ferric trichloride (FeCl3) modified biochar (rice, wheat, and corn straw biochar) in Cd-As co-contaminated soil, with application rates of 1, 5, and 10% (w/w) and the incubation of 1, 7, 10, and 15 days. Using TCLP (Toxicity Characteristic Leaching Procedure) method, 10% of FeCl3 modified corn-straw derived biochar (FCB) had the highest immobilization efficiency of Cd(II) (63.21%) and As(V) (95.10%) after 10 days of the incubation. Iron-modified biochar immobilized higher fractions of water-soluble (F1) and surface-absorbed (F2) metal fractions than pristine biochar. For FCB amendment, Cd was mostly presented in the organic matter (OM) and sulfides associated (F4) and residual (F5) fractions (88.52%), as was found in the Fe-Al (oxides and hydroxides) (F3), F4, and F5 fractions (75.87%). FCB amendment increased soil pH values and available iron contents (p < 0.05), while no changes in soil available phosphorus content (p > 0.05). This study showed that FCB application reduces the environmental mobility of metals in Cd-As contaminated soil, while it also increases soil pH and available nutrient mobility, improving soil environmental quality and reducing remediation costs.
Parental Beliefs and Feelings about Autism Spectrum Disorder in Iran
Authors: Samadi Sayyed Ali
Published: January 2020

Background: This study provides information on beliefs that parents of children with autism hold in Iran. The main focus is on their beliefs about the cause and the way that this condition is explained based on the first signs that made them be concerned for their children. Method: To attain the aims of this study, 43 parents of children with ASD (27 mothers and 16 fathers) were recruited and interviewed in two sessions in their home. A mixed method approach was used to understand Iranian parents’ reaction to receive diagnosis for their children. Results: Based on findings for the identification, description, and treatment of ASD in Iran, it is argued that since Iranian parents had their special justification regarding their experience with ASD, early child development and interventions must be understood within cultural context. Culturally informed research on ASD is vital to boost awareness of the importance of understanding parental concerns and their need for educational and psychological services in countries in which autism is less known, undiagnosed, misdiagnosed, or even stigmatized. Understanding the difference in ASD meaning across cultures urges stakeholders such as service providers and policymakers to accept and appreciate cultural and individual diversities in the present century.
Financial Leverage, Economic Growth and Environmental Degradation: Evidence from 30 Provinces in China
Authors: Zhao Miyun , Yang Rui , Li Yi
Published: January 2020

This study seeks to investigate the endogenous relationship between financial leverage, economic growth and environmental degradation in China by employing a the generalized moments method (GMM) panel vector autoregressive (PVAR) approach with a panel of data from China’s 30 provinces over the period 1997–2016. Three key results arise. First, financial leverage can significantly lessen economic growth, while economic growth decreases financial leverage. Second, economic growth provides an important impetus to boost carbon emissions. Finally, carbon emissions have inversely pushed up financial leverage. These results reflect to some extent China’s impressive rate of economic growth, which has been attained via continuously supporting inefficient state-owned enterprises and heavy and polluting industries through bank loans. The results are further supported by the variance decomposition. The findings provide valuable policy implications for deepening financial supply-side structure reform to transform and upgrade China’s real economy. These policy implications are conductive to developing a low-carbon economy.
Differences among Saudi and Expatriate Students: Body Composition Indices, Sitting Time Associated with Media Use and Physical Activity Pattern
Authors: Alghadir Ahmad H. , Iqbal Zaheen A. , Gabr Sami A.
Published: January 2020

Background: Being overweight at a young age is a predictor of developing obesity and related complications later in adulthood, posing a high risk to public health. Various ethnic subgroups have been identified as having a higher prevalence of overweight or obese. Saudi Arabia is one of the fastest-growing economies in the world, where the expatriate population comprises 33% of its total population. The objective of this study was to investigate differences in body composition indices, sitting time associated with media use, and physical activity pattern among a sample of local and expatriate school students in Saudi Arabia. Methods: 500 students (aged 8–18 years) from various schools were invited to participate in this study. Body weight, waist circumference (WC) and height were measured using a portable digital metric scale, standard measuring tape and wall mounted tape respectively. Participants and their parents were jointly asked to report the average time that the participant spent sitting using media (watching TV, playing video games, and using the internet and other screen-based devices etc.) per day. The pattern of physical activity among participants was measured using a short form of the International Physical Activity Questionnaire (IPAQ). Basal metabolic rate (BMR) and total daily energy expenditure (TEE) were estimated from body weight, height, age, sex and physical activity, according to the Harris–Benedict equation. Results: Data from 450 (90%) of the participants were used for analysis. The mean age of the participants was 14.55 ± 1.74 years. Body mass index (BMI), WC, waist to height ratio (WHtR), BMR and TEE differed significantly among the participants. Physical fitness score negatively correlated with BMI and WC, while sitting time associated with media use positively correlated with BMI, WC, WHtR and physical fitness score, among both Saudi and expatriate participants. Conclusions: Body composition indices and sitting time associated with media use were higher among Saudi boys and expatriate girls. Expatriate boys and girls were reported to be physically more active than their Saudi counterparts. BMR and TEE were higher among expatriate boys and Saudi girls. Although this study provides useful information about the association of body composition indices, sitting time associated with media use, and physical activity pattern among local and expatriate school students in SA, similar studies involving a larger study sample, with equal gender representation, are further required to determine various factors associated with this link.
Association between Sexual Satisfaction and Depression and Anxiety in Adolescents and Young Adults
Authors: Carcedo Rodrigo J. , Fernández-Rouco Noelia , Fernández-Fuertes Andrés A. , Martínez-Álvarez José Luis
Published: January 2020

The role of sexual satisfaction in adolescents and young adults’ mental health has not been thoroughly investigated. The aim of this work is to study differences in sexual satisfaction and mental health (anxiety and depression) based on romantic relationship status (having a partner vs. not having one) and gender. Likewise, the association between sexual satisfaction and mental health and the moderating effect of romantic relationship status and gender was addressed in this research. A total of 1682 Spanish adolescents (14–17) and young adults (18–29) agreed to participate in this cross-sectional investigation. Two-factor ANOVA and MANOVA, and hierarchical regression models were utilized in this study. In general, results showed more difficulties in sexual satisfaction and mental health for those not in a current relationship and for women. Additionally, higher levels of sexual satisfaction was associated with lower levels of anxiety for adolescents and lower levels of depression for young adults. These associations were stronger for those in a current relationship. This study highlights the importance of sexual satisfaction as a modifying factor against mental health problems, especially in the context of a current romantic relationship.
Personal Exposure Estimates via Portable and Wireless Sensing and Reporting of Particulate Pollution
Authors: Agrawaal Harsshit , Jones Courtney , Thompson J.E.
Published: January 2020

Low-cost, portable particle sensors (n = 3) were designed, constructed, and used to monitor human exposure to particle pollution at various locations and times in Lubbock, TX. The air sensors consisted of a Sharp GP2Y1010AU0F dust sensor interfaced to an Arduino Uno R3, and a FONA808 3G communications module. The Arduino Uno was used to receive the signal from calibrated dust sensors to provide a concentration (µg/m3) of suspended particulate matter and coordinate wireless transmission of data via the 3G cellular network. Prior to use for monitoring, dust sensors were calibrated against a reference aerosol monitor (RAM-1) operating independently. Sodium chloride particles were generated inside of a 3.6 m3 mixing chamber while the RAM-1 and each dust sensor recorded signals and calibration was achieved for each dust sensor independently of others by direct comparison with the RAM-1 reading. In an effort to improve the quality of the data stream, the effect of averaging replicate individual pulses of the Sharp sensor when analyzing zero air has been studied. Averaging data points exponentially reduces standard deviation for all sensors with n < 2000 averages but averaging produced diminishing returns after approx. 2000 averages. The sensors exhibited standard deviations for replicate measurements of 3–6 µg/m3 and corresponding 3σ detection limits of 9–18 µg/m3 when 2000 pulses of the dust sensor LED were averaged over an approx. 2 min data collection/transmission cycle. To demonstrate portable monitoring, concentration values from the dust sensors were sent wirelessly in real time to a ThingSpeak channel, while tracking the sensor’s latitude and longitude using an on-board Global Positioning System (GPS) sensor. Outdoor and indoor air quality measurements were made at different places and times while human volunteers carried sensors. The measurements indicated walking by restaurants and cooking at home increased the exposure to particulate matter. The construction of the dust sensors and data collected from this research enhance the current research by describing an open-source concept and providing initial measurements. In principle, sensors can be massively multiplexed and used to generate real-time maps of particulate matter around a given location.
Factors Affecting Painkillers, Sedatives/Hypnotics, Nicotine, and Unhealthy Alcohol Use Among Gay and Bisexual Men in Taiwan
Authors: Li Dian-Jeng , Chen Shiou-Lan , Chang Yu-Ping , Yen Cheng-Fang
Published: January 2020

Substance use has become a major health problem globally for sexual minorities. However, few studies have explored multi-dimensional factors associated with smoking, drinking, and prescription drug use. We aimed to investigate the factors affecting painkiller, sedative/hypnotic, nicotine and unhealthy alcohol use among gay and bisexual men in Taiwan. We recruited 500 gay or bisexual men and assessed their experiences of using painkillers, sedatives/hypnotics, nicotine, alcohol and multi-dimensional factors with self-reported questionnaires. Multivariate logistic regression with a forward stepwise model was used to verify the factors associated with substance use. Overall, 9.4%, 5.4%, and 13.8% of the participants reported using painkillers, sedatives/hypnotics, and nicotine, respectively, and 5.6% reported unhealthy alcohol use. Victims of traditional homophobic bullying in childhood and adolescence were more likely to report nicotine use, sedative/hypnotic use, and unhealthy alcohol use in early adulthood than non-victims. Missing classes or truancy at senior high school was associated with painkiller and sedative/hypnotic use in early adulthood. Traditional homophobic bullying and missing classes or truancy in childhood and adolescence predicted substance use in early adulthood among the gay and bisexual men in this study. Timely preventions and interventions for substance use are crucial for gay and bisexual men, especially for those who experience homophobic bullying and missing classes or truancy.
Trajectories of Outpatient Service Utilisation of Hypertensive Patients in Tertiary Hospitals in China
Authors: Pan Zijing , Xu Wanchun , Li Zhong , Xu Chengzhong , Lu Fangfang , Zhang Pei , Zhang Liang , Ye Ting
Published: January 2020

This study aims to identify the characteristics and trajectories of outpatient service utilisation for hypertensive patients in tertiary hospitals. This study also attempts to investigate the determinants of the trajectories of outpatient service utilisation. A total of 9822 patients with hypertension and hypertension-related medical utilisation were recruited in Yichang, China from January 1 to December 31 in 2016. The latent trajectories of outpatient service utilisation were identified through latent class growth analysis. Differences in the demographic characteristics and medical utilisation among patients in different trajectories were tested by one-way ANOVA and chi-square analysis. The predictors of the trajectory groups of outpatient service utilisation were identified through multinomial logistic regression. Four trajectory groups were determined as stable-low (34.7%), low-fluctuating (13.4%), high-fluctuating (22.5%), and stable-high (29.4%). Significant differences were observed in all demographic characteristics (p < 0.001) and medical service utilisation variables (p < 0.001) among the four trajectories except for inpatient cost (p = 0.072). Determinants for outpatient service utilisation patterns include the place of residence, education level, outpatient visit times, inpatient service utilisation, and outpatient cost. Overall, hypertensive patients visiting outpatient units in the tertiary hospital were middle-aged, elderly, and well-educated, and they received poor follow-up services. The four identified latent trajectories have different characteristics and medical utilisation patterns. Trajectory group-based measurements are necessary for hypertension management and economic burden reduction.
Transcriptome Analysis of Rice Roots in Response to Root-Knot Nematode Infection
Authors: Zhou Yuan , Zhao Di , Shuang Li , Xiao Dongxue , Xuan Yuanhu , Duan Yuxi , Chen Lijie , Wang Yuanyuan , Liu Xiaoyu , Fan Haiyan , Zhu Xiaofeng
Published: January 2020

Meloidogyne incognita and Meloidogyne graminicola are root-knot nematodes (RKNs) infecting rice (Oryza sativa L.) roots and severely decreasing yield, whose mechanisms of action remain unclear. We investigated RKN invasion and development in rice roots through RNA-seq transcriptome analysis. The results showed that 952 and 647 genes were differently expressed after 6 (invasion stage) and 18 (development stage) days post inoculation, respectively. Gene annotation showed that the differentially expressed genes were classified into diverse metabolic and stress response categories. Furthermore, phytohormone, transcription factor, redox signaling, and defense response pathways were enriched upon RKN infection. RNA-seq validation using qRT-PCR confirmed that CBL-interacting protein kinase (CIPK) genes (CIPK5, 8, 9, 11, 14, 23, 24, and 31) as well as brassinosteroid (BR)-related genes (OsBAK1, OsBRI1, D2, and D11) were altered by RKN infection. Analysis of the CIPK9 mutant and overexpressor indicated that the RKN populations were smaller in cipk9 and larger in CIPK9 OX, while more galls were produced in CIPK9 OX plant roots than the in wild-type roots. Significantly fewer numbers of second-stage infective juveniles (J2s) were observed in the plants expressing the BR biosynthesis gene D2 mutant and the BR receptor BRI1 activation-tagged mutant (bri1-D), and fewer galls were observed in bri1-D roots than in wild-type roots. The roots of plants expressing the regulator of ethylene signaling ERS1 (ethylene response sensor 1) mutant contained higher numbers of J2s and developed more galls compared with wild-type roots, suggesting that these signals function in RKN invasion or development. Our findings broaden our understanding of rice responses to RKN invasion and provide useful information for further research on RKN defense mechanisms.
Optimization of Chitosan–Alginate Microparticles for Delivery of Mangostins to the Colon Area Using Box–Behnken Experimental Design
Authors: Mulia Kamarza , Singarimbun Ameninta Cesanina , Krisanti Elsa Anisa
Published: January 2020

Chitosan-alginate microparticles loaded with hydrophobic mangostins present in the mangosteen rind extract have been formulated and optimized for colon-targeted bioactive drug delivery systems. The chitosan–mangostin microparticles were prepared using the ionotropic gelation method with sodium tripolyphosphate as the cross-linking agent of chitosan. The chitosan–mangostin microparticles were then encapsulated in alginate with calcium chloride as the linking agent. The mangostin release profile was optimized using the Box–Behnken design for response surface methodology with three independent variables: (A) chitosan–mangostin microparticle size, (B) alginate:chitosan mass ratio, and (C) concentration of calcium chloride. The following representative equation was obtained: percent cumulative release of mangostins (10 h) = 59.51 − 5.16A + 20.00B − 1.27C − 1.70AB − 5.43AC − 5.04BC + 0.0579A2 + 10.25B2 + 1.10C2. Cumulative release of 97% was obtained under the following optimum condition for microparticle preparation: chitosan–mangosteen particle size < 100 µm, alginate:chitosan mass ratio of 0.5, and calcium chloride concentration of 4% w/v. The alginate to chitosan mass ratio is the statistically significant variable in the optimization of sequential release profile of mangostins in simulated gastrointestinal fluids. Furthermore, a sufficient amount of alginate is necessary to modify the chitosan microparticles and to achieve a complete release of mangostins. The results of this work indicate that the complete release of mangostins to the colon area can be achieved using the chitosan–alginate microparticles as the bioactive delivery system.
Wind Turbines Offshore Foundations and Connections to Grid
Authors: Manzano-Agugliaro Francisco , Sánchez-Calero Miguel , Alcayde Alfredo , San-Antonio-Gómez Carlos , Perea-Moreno Alberto-Jesús , Salmeron-Manzano Esther
Published: January 2020

Most offshore wind farms built thus far are based on waters below 30 m deep, either using big diameter steel monopiles or a gravity base. Now, offshore windfarms are starting to be installed in deeper waters and the use of these structures—used for oil and gas like jackets and tripods—is becoming more competitive. Setting aside these calls for direct or fixed foundations, and thinking of water depths beyond 50 m, there is a completely new line of investigation focused on the usage of floating structures; TLP (tension leg platform), Spar (large deep craft cylindrical floating caisson), and semisubmersible are the most studied. We analyze these in detail at the end of this document. Nevertheless, it is foreseen that we must still wait sometime before these solutions, based on floating structures, can become truth from a commercial point of view, due to the higher cost, rather than direct or fixed foundations. In addition, it is more likely that some technical modifications in the wind turbines will have to be implemented to improve their function. Regarding wind farm connections to grid, it can be found from traditional designs such as radial, star or ring. On the other hand, for wind generator modeling, classifications can be established, modeling the wind turbine and modeling the wind farm. Finally, for the wind generator control, the main strategies are: passive stall, active stall, and pitch control; and when it is based on wind generation zone: fixed speed and variable speed. Lastly, the trend is to use strategies based on synchronous machines, as the permanent magnet synchronous generator (PMSG) and the wound rotor synchronous generator (WRSG).
The Effect of Processing Variables on Powder Interlayer Bonding in Nickel-Based Superalloys
Authors: Stanners Olivia , John Sean , Davies Helen M. , Watkins Ieuan , Marchisio Silvia
Published: January 2020

Powder Interlayer Bonding (PIB) has been considered as a lower-energy joining technology for nickel-based superalloys compared to conventional methods; such as friction welding. Typically; nickel-based superalloys exhibit high energy requirements for joining due to their high operating temperatures. However; PIB utilizes a localized temperature gradient created by an induction current; reducing the energy requirements for the process. PIB is a solid-state joining method that compresses and heats a powder interlayer between two faying surfaces to produce one joined workpiece. It has been successfully used to bond titanium alloys; and the objectives of this work were to explore its application as a joining method for nickel-based superalloys. Initial results showed that joining nickel-based superalloys via PIB is possible; and bondlines with very little porosity were observed. Further analysis showed that these bonded areas had lower porosity than the base material; suggesting PIB could be a successful joining method for difficult-to-join nickel-based superalloys.
Flexural Behavior of a Precast Concrete Deck Connected with Headed GFRP Rebars and UHPC
Authors: Chin Won Jong , Park Young Hwan , Cho Jeong-Rae , Lee Jin-Young , Yoon Young-Soo
Published: January 2020

Steel bent reinforcing bars (rebars) are widely used to provide adequate anchorage. Bent fiber-reinforced polymer (FRP) rebars are rarely used because of the difficulty faced during the bending process of the FRP rebars at the construction site. Additionally, the bending process may cause a significant decrease in the structural performance of the FRP rebars. Therefore, to overcome these drawbacks, a headed glass fiber-reinforced polymer (GFRP) rebar was developed in this study. The pull-out tests of the headed GFRP rebars with diameters of 16 and 19 mm were conducted to evaluate their bond properties in various cementitious materials. Moreover, structural flexural tests were conducted on seven precast concrete decks connected with the headed GFRP rebars and various cementitious fillers to estimate the flexural behavior of the connected decks. The results demonstrate that the concrete decks connected with the headed GFRP rebar and ultra-high-performance concrete (UHPC) exhibited improved flexural performance.
Preceramic Paper-Derived SiCf/SiCp Composites Obtained by Spark Plasma Sintering: Processing, Microstructure and Mechanical Properties
Authors: Li Ke , Kashkarov Egor , Syrtanov Maxim , Sedanova Elizaveta , Ivashutenko Alexander , Lider Andrey , Fan Ping , Yuan Daqing , Travitzky Nahum
Published: January 2020

Ceramic matrix composites (CMCs) based on silicon carbide (SiC) are promising materials for applications as structural components used under high irradiation flux and high temperature conditions. The addition of SiC fibers (SiCf) may improve both the physical and mechanical properties of CMCs and lead to an increase in their tolerance to failure. This work describes the fabrication and characterization of novel preceramic paper-derived SiCf/SiCp composites fabricated by spark plasma sintering (SPS). The sintering temperature and pressure were 2100 °C and 20–60 MPa, respectively. The content of fibers in the composites was approx. 10 wt.%. The matrix densification and fiber distribution were examined by X-ray computed tomography and scanning electron microscopy. Short processing time avoided the destruction of SiC fibers during SPS. The flexural strength of the fabricated SiCf/SiCp composites at room temperature varies between 300 and 430 MPa depending on the processing parameters and microstructure of the fabricated composites. A quasi-ductile fracture behavior of the fabricated composites was observed.
Photoprotective Activity of Topsentin, A Bis(Indole) Alkaloid from the Marine Sponge Spongosorites genitrix, by Regulation of COX-2 and Mir-4485 Expression in UVB-Irradiated Human Keratinocyte Cells
Authors: Hwang Jiyoung , Kim Donghwa , Park Jae Sung , Park Hyen Joo , Shin Jongheon , Lee Sang Kook
Published: January 2020

Skin is an important barrier to protect the body from environmental stress. However, exposure to ultraviolet radiation (UV) and various environmental oxidative stresses can cause skin inflammation. Cyclooxygenase-2 (COX-2) is an inducible enzyme that mediates the formation of prostaglandin E2 (PGE2) against internal and external inflammatory stimulations. Therefore, the inhibition of COX-2 is an important approach to maintain skin health and prevent skin inflammation and carcinogenesis. Topsentin, a bis(indolyl)imidazole alkaloid isolated from the marine sponge Spongosorites genitrix, has been reported to exhibit anti-tumor and anti-microbial activities. However, the effect of topsentin on skin inflammation and its underlying molecular mechanism has not been elucidated. In the present study, we identified the photoprotective effects of topsentin on UVB irradiated human epidermal keratinocyte HaCaT cells. Topsentin suppresses COX-2 expression and its upstream signaling pathways, AP-1 and MAPK. Furthermore, topsentin inhibits miR-4485, a new biomarker selected from a microarray, and its target gene tumor necrosis factor alpha induced protein 2 (TNF-α IP2). The photoprotective effect of topsentin was also confirmed in a reconstructed human skin model. These findings suggest that topsentin may serve as a potential candidate for cosmetic formulations with skin inflammatory-mediated disorder.
Spirulina Platensis Supplementation Coupled to Strength Exercise Improves Redox Balance and Reduces Intestinal Contractile Reactivity in Rat Ileum
Authors: Araujo Layanne C. C. , Brito Aline F. , Souza Iara L. L. , Ferreira Paula B. , Vasconcelos Luiz Henrique C. , Silva Alexandre S. , Silva Bagnólia A.
Published: January 2020

The blue alga Spirulina platensis has presented several pharmacological activities, highlighting its actions as an anti-inflammatory and antioxidant. In addition, there are few studies with the influence of strength training on physiological parameters, as intestinal contractility and oxidative cell damage. We evaluated the influence of S. platensis supplementation, strength training, and its association on contractile reactivity of rat ileum, as well as the balance of oxidative stress/antioxidant defenses. Methods: Male Wistar rats were divided into; sedentary (S); S + supplemented with algae at 50 (S50), 150 (S150), and 500 mg/kg (S500); trained (T); and T + supplemented (T50, T150, and T500). Contractile reactivity was analyzed by kymographs; oxidative stress on ileum by the malondialdehyde (MDA) formation; and the antioxidant capacity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. S. platensis supplementation reduced the reactivity of rat ileum to carbachol (CCh) and KCl, while training reduced only the CCh efficacy. In addition, association potentiated the reduction on contractile reactivity. Supplementation reduced the oxidative stress and increased oxidation inhibition; training alone did not alter this parameter, however association potentiated this beneficial effect. Therefore, this study demonstrated that both supplementation and its association with strength training promote beneficial effects regarding intestinal contractile reactivity and oxidative stress, providing new insights for intestinal disorders management.
Long-Term Mesophilic Anaerobic Co-Digestion of Swine Manure with Corn Stover and Microbial Community Analysis
Authors: Wang Haipeng , Lim Teng Teeh , Duong Cuong , Zhang Wei , Xu Congfeng , Yan Lei , Mei Zili , Wang Weidong
Published: January 2020

Long-term anaerobic co-digestion of swine manure (SM) and corn stover (CS) was conducted using semi-continuously loaded digesters under mesophilic conditions. A preliminary test was first conducted to test the effects of loading rates, and results indicated the 3 g-VS L−1 d−1 was the optimal loading rate. Based on the preliminary results, a verification replicated test was conducted with 3 g-VS L−1 d−1 loading rate and different SM/CS ratios (1:1, 2:1 and 1:2). Results showed that a SM/CS ratio of 2/1 was optimal, based on maximum observed methane-VSdes generation and carbon conversion efficiency (72.56 ± 3.40 mL g−1 and 40.59%, respectively). Amplicon sequencing analysis suggested that microbial diversity was increased with CS loading. Amino-acid-degrading bacteria were abundant in the treatment groups. Archaea Methanoculleus could enhance biogas and methane productions.
Iron(II) Spin Crossover Complexes with 4,4′-Dipyridylethyne—Crystal Structures and Spin Crossover with Hysteresis
Authors: Dankhoff Katja , Lochenie Charles , Weber Birgit
Published: January 2020

Three new iron(II) 1D coordination polymers with cooperative spin crossover behavior showing thermal hysteresis loops were synthesized using N2O2 Schiff base-like equatorial ligands and 4,4′-dipyridylethyne as a bridging, rigid axial linker. One of those iron(II) 1D coordination polymers showed a 73 K wide hysteresis below room temperature, which, upon solvent loss, decreased to a still remarkable 30 K wide hysteresis. Single crystal X-ray structures of two iron(II) coordination polymers and T-dependent powder XRD patterns are discussed to obtain insight into the structure property relationship of those materials.
Induced Hydrophilicity and In Vitro Preliminary Osteoblast Response of Polyvinylidene Fluoride (PVDF) Coatings Obtained via MAPLE Deposition and Subsequent Thermal Treatment
Authors: Dumitrescu Luminita Nicoleta , Neacsu Patricia , Necula Madalina G. , Bonciu Anca , Marascu Valentina , Cimpean Anisoara , Moldovan Antoniu , Rotaru Andrei , Dinca Valentina , Dinescu Maria
Published: January 2020

Recent advancements in biomedicine have focused on designing novel and stable interfaces that can drive a specific cellular response toward the requirements of medical devices or implants. Among these, in recent years, electroactive polymers (i.e., polyvinylidene fluoride or PVDF) have caught the attention within the biomedical applications sector, due to their insolubility, stability in biological media, in vitro and in vivo non-toxicity, or even piezoelectric properties. However, the main disadvantage of PVDF-based bio-interfaces is related to the absence of the functional groups on the fluoropolymer and their hydrophobic character leading to a deficiency of cell adhesion and proliferation. This work was aimed at obtaining hydrophilic functional PVDF polymer coatings by using, for the first time, the one-step, matrix-assisted pulsed evaporation (MAPLE) method, testing the need of a post-deposition thermal treatment and analyzing their preliminary capacity to support MC3T3-E1 pre-osteoblast cell survival. As osteoblast cells are known to prefer rough surfaces, MAPLE deposition parameters were studied for obtaining coatings with roughness of tens to hundreds of nm, while maintaining the chemical properties similar to those of the pristine material. The in vitro studies indicated that all surfaces supported the survival of viable osteoblasts with active metabolisms, similar to the “control” sample, with no major differences regarding the thermally treated materials; this eliminates the need to use a secondary step for obtaining hydrophilic PVDF coatings. The physical-chemical characteristics of the thin films, along with the in vitro analyses, suggest that MAPLE is an adequate technique for fabricating PVDF thin films for further bio-applications.
ICT and AIE Characteristics Two Cyano-Functionalized Probes and Their Photophysical Properties, DFT Calculations, Cytotoxicity, and Cell Imaging Applications
Authors: Tarai Arup , Huang Meina , Das Pintu , Pan Wenhui , Zhang Jianguo , Gu Zhenyu , Yan Wei , Qu Junle , Yang Zhigang
Published: January 2020

Two probes, AIE-1 and AIE-2, were synthesized to investigate the effect of substitutional functional group on aggregation (aggregation-caused quenching (ACQ) or aggregation-induced emission (AIE)) and intramolecular charge transfer (ICT) behavior as well as on the cell imaging aspect. The yellow-color non-substituted probe AIE-1 showed weak charge-transfer absorption and an emission band at 377 nm and 432 nm, whereas the yellowish-orange color substituted probe AIE-2 showed a strong charge-transfer absorption and an emission band at 424 nm and 477 nm in THF solvent. The UV-Vis studies of AIE-1 and AIE-2 in THF and THF with different water fractions showed huge absorption changes in AIE-2 with high water fractions due to its strong aggregation behavior, but no such noticeable absorption changes were observed for AIE-1. Interestingly, the fluorescence intensity of AIE-1 at 432 nm gradually decreased with increasing water fractions and became almost non-emissive at 90% water. However, the monomer-type emission of AIE-2 at 477 nm was shifted to 584 nm with a 6-fold increase in fluorescence intensity in THF-H2O (1:9, v/v) solvent mixtures due to the restriction of intramolecular rotation on aggregation in high water fractions. This result indicates that the probe AIE-1 shows ACQ and probe AIE-2 shows AIE behaviors in THF-H2O solvent mixtures. Furthermore, the emission spectra of AIE-1 and AIE-2 were carried out in different solvent and with different concentrations to see the solvent- or concentration-dependent aggregation behavior. Scanning electron microscope (SEM) and dynamic light scattering (DLS) experiments were also conducted to assess the morphology and particle size of two probes before and after aggregation. Both of the probes, AIE-1 and AIE-2, showed less toxicity on HeLa cells and were suitable for cell imaging studies. Density functional theory (DFT) calculation was also carried out to confirm the ICT process from an electron-rich indole moiety to an electron-deficient cyano-phenyl ring of AIE-1 or AIE-2.
Cytotoxic and Anti-Inflammatory Effects of Ent-Kaurane Derivatives Isolated from the Alpine Plant Sideritis hyssopifolia
Authors: Aimond Axelle , Calabro Kevin , Audoin Coralie , Olivier Elodie , Dutot Mélody , Buron Pauline , Rat Patrice , Laprévote Olivier , Prado Soizic , Roulland Emmanuel , Thomas Olivier P. , Genta-Jouve Grégory
Published: January 2020

This paper reports the isolation and structural characterization of four new ent-kaurane derivatives from the Lamiaceae plant Sideritis hyssopifolia. Planar structures and relative configurations were determined using both mass spectrometry and nuclear magnetic resonance (1D and 2D). Absolute configurations were determined by comparing experimental and theoretical electronic circular dichroism spectra. The cytotoxic and microbial activities of all new compounds were tested. Compounds that were non-cytotoxic were further evaluated for anti-inflammatory activity.
The Possible Role of the Nitroso-Sulfide Signaling Pathway in the Vasomotoric Effect of Garlic Juice
Authors: Berenyiova Andrea , Grman Marian , Misak Anton , Golas Samuel , Cuchorova Justina , Cacanyiova Sona
Published: January 2020

The beneficial cardiovascular effects of garlic have been reported in numerous studies. The major bioactive properties of garlic are related to organic sulfides. This study aimed to investigate whether garlic juice works exclusively due to its sulfur compounds or rather via the formation of new products of the nitroso-sulfide signaling pathway. Changes in isometric tension were measured on the precontracted aortic rings of adult normotensive Wistar rats. We evaluated NO-donor (S-nitrosoglutathione, GSNO)-induced vasorelaxation and compare it with effects of hydrogen sulfide (H2S)/GSNO and garlic/GSNO. Incubation with garlic juice increased the maximal GSNO-induced relaxation and markedly changed the character of the relaxant response. Although incubation with an H2S donor enhanced the maximal vasorelaxant response of GSNO, neither the absolute nor the relative relaxation changed over time. The mixture of GSNO with an H2S donor evoked a response similar to GSNO-induced relaxation after incubation with garlic juice. This relaxation of the H2S and GSNO mixture was soluble guanylyl cyclase (sGC) dependent, partially reduced by HNO scavenger and it was adenosine triphosphate-sensitive potassium channels (KATP) independent. In this study, we demonstrate for the first time the suggestion that H2S itself is probably not the crucial bioactive compound of garlic juice but rather potentiates the production of new signaling molecules during the GSNO-H2S interaction.
Gut Microbiota and Its Metabolites in Atherosclerosis Development
Authors: Pieczynska Magdalena D. , Yang Yang , Petrykowski S. , Horbanczuk Olaf K. , Atanasov Atanas G. , Horbanczuk Jaroslaw O.
Published: January 2020

Gut microbiota metabolites have a great influence on host digestive function and body health itself. The effects of intestinal microbes on the host metabolism and nutrients absorption are mainly due to regulatory mechanisms related to serotonin, cytokines, and metabolites. Multiple studies have repeatedly reported that the gut microbiota plays a fundamental role in the absorption of bioactive compounds by converting dietary polyphenols into absorbable bioactive substances. Moreover, some intestinal metabolites derived from natural polyphenol products have more biological activities than their own fundamental biological functions. Bioactive like polyphenolic compounds, prebiotics and probiotics are the best known dietary strategies for regulating the composition of gut microbial populations or metabolic/immunological activities, which are called “three “p” for gut health”. Intestinal microbial metabolites have an indirect effect on atherosclerosis, by regulating lipid metabolism and inflammation. It has been found that the diversity of intestinal microbiota negatively correlates with the development of atherosclerosis. The fewer the variation and number of microbial species in the gut, the higher the risk of developing atherosclerosis. Therefore, the atherosclerosis can be prevented and treated from the perspective of improving the number and variability of gut microbiota. In here, we summarize the effects of gut metabolites of natural products on the pathological process of the atherosclerosis, since gut intestinal metabolites not only have an indirect effect on macrophage foaming in the vessel wall, but also have a direct effect on vascular endothelial cells.
Galangin Protects against Symptoms of Dextran Sodium Sulfate-Induced Acute Colitis by Activating Autophagy and Modulating the Gut Microbiota
Authors: Xuan Hongzhuan , Ou Aiqun , Hao Shengyu , Shi Jiajun , Jin Xiaolu
Published: January 2020

Galangin is a natural flavonoid that has been reported to provide substantial health benefits. Nevertheless, little is known about the potential effects of galangin against inflammatory bowel diseases. Here, an in vivo study was performed to investigate the preventive effects of galangin against dextran sulphate sodium (DSS)-induced acute murine colitis, which mimics the symptoms of human ulcerative colitis (UC). Pre-treatment with galangin (15 mg/kg, p.o.) resulted in a significant decreased in the macroscopic signs of DSS-induced colitic symptoms, including a decreased disease activity index, prevention of the colon length shortening, and alleviation of the pathological changes occurring in the colon. Colonic pro-inflammatory mediators, including tumor necrosis factor-alpha, interleukin (IL)-1 beta, and IL-6, as well as myeloperoxidase activities were decreased following galangin pre-treatment when compared with the DSS control group. Moreover, galangin pre-treatment significantly increased the expressions of autophagy-related proteins and promoted the formation of autophagosome in the colon. Galangin pre-treatment increased the diversity of the gut microbiota, and this was accompanied by increased levels of short-chain fatty acids. These observed changes could involve the modulating effects conferred by galangin in relation to some specific bacteria populations, including the recovery of Lactobacillus spp., and increased Butyricimonas spp. Overall, these results support the use of galangin in the prevention of UC.
Non-Contact Damage Detection under Operational Conditions with Multipoint Laservibrometry
Authors: Cao Xiaodong , Rembe Christian
Published: January 2020

Scanning laser–Doppler vibrometry (SLDV) can localize and visualize damages in mechanical structures. In order to enable scanning, it is necessary to repeat the vibration. Therefore, this technique is not suited to detect emerging hazards in working machinery that change the vibration behavior. A common technique for such cases is monitoring the vibration excited by machine operation with accelerometers. This technique requires mechanical coupling between sensors and the measurement object, which influences the high-frequency vibration responses. However, in the low-frequency range, local damages do not shift resonances or distort operational deflection shapes (ODS) significantly. These alterations in the vibration behavior are tiny and hard to detect. This paper shows that multipoint laservibrometry (MPV) with laser excitation can measure these effects efficiently, and it further demonstrates that damages influence ODSs at frequencies above 20 kHz much stronger than at frequencies below 20 kHz. In addition, ODS-based damage indices are discussed; these are highly sensitive to minute visible changes of the ODSs. In order to enhance the sensitivity of hazard detection, the response vector assurance criterion value is computed and evaluated during operation. The capabilities and limitations of the methodology on the example of a cantilever with manually emerging damage are demonstrated.
A Refractive Index Sensor Based on H-Shaped Photonic Crystal Fibers Coated with Ag-Graphene Layers
Authors: Li Tianshu , Zhu Lianqing , Yang Xianchao , Lou Xiaoping , Yu Liandong
Published: January 2020

An Ag-graphene layers-coated H-shaped photonic crystal fiber (PCF) surface plasmon resonance (SPR) sensor with a U-shaped grooves open structure for refractive index (RI) sensing is proposed and numerically simulated by the finite element method (FEM). The designed sensor could solve the problems of air-holes material coating and analyte filling in PCF. Two big air-holes in the x-axis produce a birefringence phenomenon leading to the confinement loss and sensitivity of x-polarized light being much stronger than y-polarized. Graphene is deposited on the layer of silver in the grooves; its high surface to volume ratio and rich π conjugation make it a suitable dielectric layer for sensing. The effect of structure parameters such as air-holes size, U-shaped grooves depth, thickness of the silver layer and number of graphene layers on the sensing performance of the proposed sensor are numerical simulated. A large analyte RI range from 1.33 to 1.41 is calculated and the highest wavelength sensitivity is 12,600 nm/RIU. In the linear RI sensing region of 1.33 to 1.36; the average wavelength sensitivity we obtained can reach 2770 nm/RIU with a resolution of 3.61 × 10−5 RIU. This work provides a reference for developing a high-sensitivity; multi-parameter measurement sensor potentially useful for water pollution monitoring and biosensing in the future.