bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased fecal ethanol and enriched ethanol-producing gut bacteria Limosilactobacillus fermentum, Enterocloster bolteae, Mediterraneibacter gnavus and Streptococcus mutans in nonalcoholic steatohepatitis
Authors: Mbaye Babacar , Magdy Wasfy Reham , Borentain Patrick , Tidjani Alou Maryam , Mottola Giovanna , Bossi Vincent , Caputo Aurelia , Gerolami Rene , Million Matthieu
Published: November 2023

Background

Non-alcoholic steatohepatitis (NASH) has become a major public health issue as one of the leading causes of liver disease and transplantation worldwide. The instrumental role of the gut microbiota is emerging but still under investigation. Endogenous ethanol (EtOH) production by gut bacteria and yeasts is an emerging putative mechanism. Microbial metagenomics and culture studies targeting enterobacteria or yeasts have been reported, but no culturomics studies have been conducted so far.

Aim

To assess fecal EtOH and other biochemical parameters, characterize NASH-associated dysbiosis and identify EtOH-producing gut microbes associated with the disease, fecal samples from 41 NASH patients and 24 controls were analyzed. High-performance liquid chromatography (HPLC) was used for EtOH, glucose, total proteins, triglyceride and total cholesterol. Viable bacteria were assessed with microbial culturomics. Microbial genetic material was assessed using 16S metagenomics targeting the hypervariable V3V4 region.

Results

Fecal EtOH and glucose was elevated in the stools of NASH patients (p < 0.05) but not triglyceride, total cholesterol or proteins. In culturomics, EtOH-producing Enterocloster bolteae and Limosilactobacillus fermentum were enriched in NASH. V3V4 16S rRNA amplicon sequencing confirmed the enrichment in EtOH-producing bacteria including L. fermentum, Mediterraneibacter gnavus and Streptococcus mutans, species previously associated with NASH and other dysbiosis-associated diseases. Strikingly, E. bolteae was identified only by culturomics. The well-known Lacticaseibacillus casei was identified in controls but never isolated in patients with NASH (p < 0.05).

Conclusion

Elevated fecal EtOH and glucose is a feature of NASH. Several different EtOH-producing gut bacteria may play an instrumental role in the disease. Culturomics and metagenomics, two complementary methods, will be critical to identify EtOH-producing bacteria for future diagnostic markers and therapeutic targets for NASH. Suppression of EtOH-producing gut microbes and L. casei administration are options to be tested in NASH treatment.
Crisis spreading in multinational firms’ network: the dual influence of local interactions
Authors: Tsouchnika Maria , Kanetidis Michael , Argyrakis Panos , Rozenblat Celine
Published: November 2023

This study investigates the characteristics of multinational firm’s interactions within and across activity sectors and the impact of intra-urban connections, during crisis propagation. By employing data that reflect ownership relations aggregated on the city level, we constructed the partial-multiplex, directed network of cities, divided into five layers by activity sector. The network was examined in two states: one excluding intra-urban interactions and one including them. The difference between these two states highlight the significant role of intra-urban networking processes in the global economy. The five layers differ both structurally and in terms of vulnerability during crisis propagation. The Knowledge Intensive Services (KIS) layer is the densest and most populous layer of all and its firms are more likely to be owners than subsidiaries. Using a simple stochastic Susceptible-Infected-Recovered (SIR) model, we simulated a crisis diffusion on the network of cities. Our results revealed that in the absence of intra-urban connections, KIS was both the most vulnerable and most influential layer in crisis propagation. The inclusion of intra-urban links sets off a complex interplay of factors that affect diffusion outcomes in nuanced ways: while it generally enhances the impact of the crisis and the influence across layers becomes rather homogeneous it can also have a protective effect, in cases of very dense and well-connected layers, such as KIS.
Identification CCL2,CXCR2,S100A9 of the immune-related gene markers and immune infiltration characteristics of inflammatory bowel disease and heart failure via bioinformatics analysis and machine learning
Authors: Luo Xu , Wang Rui , Zhang Xin , Wen Xin , Deng Siwei , Xie Wen
Published: November 2023

Background

Recently, heart failure (HF) and inflammatory bowel disease (IBD) have been considered to be related diseases with increasing incidence rates; both diseases are related to immunity. This study aims to analyze and identify immune-related gene (IRG) markers of HF and IBD through bioinformatics and machine learning (ML) methods and to explore their immune infiltration characteristics.

Methods

This study used gene expressiondata (GSE120895, GSE21610, GSE4183) from the Gene Expression Omnibus (GEO) database to screen differentially expressed genes (DEGs) and compare them with IRGs from the ImmPort database to obtain differentially expressed immune-related genes (DIRGs). Functional enrichment analysis of IRGs was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Subsequently, three machine models and protein–protein interactions (PPIs) were established to identify diagnostic biomarkers. The receiver operating characteristic (ROC) curves were applied to evaluate the diagnostic value of the candidate biomarkersin the validation set (GSE1145, GSE36807) and obtain their correlations with immune cells through the Spearman algorithm. Finally, the CIBERSORT algorithm was used to evaluate the immune cell infiltration of the two diseases.

Results

Thirty-four DIRGs were screened and GO and KEGG analysis results showed that these genes are mainly related to inflammatory and immune responses. CCL2, CXCR2 and S100A9 were identified as biomarkers.The immune correlation results indicated in both diseases that CCL2 is positively correlated with mast cell activation, CXCR2 is positively correlated with neutrophils and S100A9 is positively correlated with neutrophils and mast cell activation. Analysis of immune characteristics showed that macrophages M2, macrophages M0 and neutrophils were present in both diseases.

Conclusions

CCL2, CXCR2 and S100A9 are promising biomarkers that will become potential immunogenetic biomarkers for diagnosing comorbidities of HF and IBD. macrophages M2, macrophages M0, neutrophil-mediated inflammation and immune regulation play important roles in the development of HF and IBD and may become diagnostic and therapeutic targets.
Case Report: It's not always about the veins; intervention of bilateral May–Thurner Syndrome secondary to iliac aneurysm
Authors: Haq Syed H. , Shah Sidra R. , Chandra Jaya , Kannan Pavithra , Patel Sandeep M.
Published: November 2023

May–Thurner Syndrome (MTS) remains evasive because of the insidiousness and variable etiologies by which it can manifest. In this study, we examine a unique presentation of MTS resulting from compression of both common iliac veins by a right common iliac artery aneurysm that required complex endovascular venous and arterial intervention.
Case Report: Successful endovascular treatment of acute type A aortic dissection
Authors: Pitts Leonard , Heck Roland , Montagner Matteo , Penkalla Adam , Kofler Markus , Falk Volkmar , Kempfert Jörg , Buz Semih
Published: November 2023

Introduction

Open surgical repair remains the current gold standard for the treatment of acute type A aortic dissection. However, especially elderly patients with relevant comorbidities who are deemed unfit for open surgery may benefit from a minimally invasive endovascular approach.

Methods

We report a case of an 80-year-old male with retrograde acute type A aortic dissection and peripheral malperfusion after receiving thoracic endovascular aortic repair due to thoracic aortic aneurysm. Our individualized endovascular approach consisted of left carotid-subclavian bypass, proximal extension of thoracic endovascular aortic repair using a covered stent graft and a single covered stent graft for the ascending aorta in combination with an uncovered stent for the aortic arch.

Results

Postoperative computed tomographic angiography demonstrated excellent outcome with no signs of endoleak or patent false lumen. Follow-up after 3.5 years showed a stable result with no signs of stent failure or dissection progress. No aortic re-interventions were needed in the further course.

Discussion

An individualized endovascular approach may be justified for acute type A aortic dissection in elderly patients with high surgical risk if performed in specialized aortic centers. Additional short-length stent graft devices are needed to address the anatomical challenges of the ascending aorta. For enhanced remodeling of the dissected aorta, the use of an additional uncovered stent may be advisable.
Age at menopause is inversely related to the prevalence of common gynecologic cancers: a study based on NHANES
Authors: Cheng Guangdong , Wang Mengmeng , Sun Hao , Lai Jingjiang , Feng Yukun , Liu Hongjin , Shang Yuwang , Zhao Yuan , Zuo Bingli , Lu Youhua
Published: November 2023

Background

The fluctuation or even loss of estrogen level caused by menopause in women, and most gynecological cancers often occur before and after menopause, so the age of menopause may be related to the occurrence of gynecological cancer.

Aim

To investigate whether the age at menopause is independently associated with the incidence of gynecological cancers and to analyze the possible influencing factors.

Methods

We selected the NHANES public database to conduct the study, and by excluding relevant influencing factors, we finally included 5706 NHANES participants who had full data on age at menopause and the occurrence of gynecologic cancers to analyze the relationship between the amount of age at menopause and gynecologic cancers based on univariate or multifactorial logistic regression analysis. Further, the relationship between age at menopause and the prevalence of different gynecologic cancers was investigated, and changes in the prevalence of different gynecologic cancers by age at menopause subgroups were observed. Finally, other relevant factors affecting the prevalence of gynecologic cancers were further investigated by subgroup analysis as well as subcluster analysis.

Results

Univariate logistic regression analysis between age at menopause and gynecologic tumor prevalence revealed a negative association between age at menopause and the prevalence of common gynecologic cancers ovarian and cervical cancer, and after adjusting for the effects of covariates, a higher risk of gynecologic tumors was found with statistically significant differences at earlier age at menopause. The regression results showed a negative association between age at menopause and gynecologic cancer prevalence in cervical and ovarian cancer patients (P<0.01,P<0.01). Cervical cancer (OR: 0.91, 95% CI: 0.87,0.94) and ovarian cancer (OR: 0.90, 95% CI: 0.86, 0.95) were more prevalent among those with younger age at menopause.

Conclusion

Age at menopause is negatively associated with the prevalence of cervical and ovarian cancers, and the earlier the age at menopause, the greater the risk of developing gynecological cancers.
Leukocyte telomere length is associated with increased risk of endometriosis: a bidirectional two-sample Mendelian randomization study
Authors: Bai Chenxue , Shen Zixiong , Qiu Binxu , Zhang Songling
Published: November 2023

Background

Endometriosis (EMs) is a common gynecological disorder. Observational studies on the relationship between leukocyte telomere length (LTL) and EMs have shown conflicting results. The purpose of this study was to evaluate the precise causal relationship between LTL and EMs using Mendelian randomization (MR) methodology.

Methods

We employed MR to assess the causal relationship between LTL and EMs. Summary data from several large-scale genome-wide association studies (GWAS) were used for bidirectional two-sample MR analysis. Sensitivity analyses were conducted to ensure the robustness of our results. All analyses were also replicated in another completely independent EMs dataset.

Results

Our MR analysis indicated that genetically predicted longer LTL increased the risk of EMs (IVW: discovery, OR=1.169, 95%CI: 1.059-1.290, p=0.002; validation, OR=1.302, 95%CI: 1.140-1.487, p=0.000), while EMs had no causal impact on LTL (IVW: discovery, OR=1.013, 95%CI: 1.000-1.027, p=0.056; IVW: validation, OR=1.005, 95%CI: 0.995-1.015, p=0.363). Causal estimates were supported by various calculation models (including MR-Egger, Weighted median, MR-PRESSO, and MR-RAPS). Heterogeneity and pleiotropy analyses also indicated robustness of the results.

Conclusion

Our findings substantiate the idea that a genetically predicted longer LTL elevates the risk of EMs, with no influence of EMs on LTL risk. This research bolsters the causal link between LTL and EMs, overcoming the constraints of earlier observational studies. It implies that LTL may potentially function as a biomarker for EMs, opening up novel possibilities for EMs prevention and treatment.
Review of multiple load forecasting method for integrated energy system
Authors: Liu Yujiao , Li Yan , Li Guoliang , Lin Yuqing , Wang Ruiqi , Fan Yunpeng
Published: November 2023

In order to further improve the efficiency of energy utilization, Integrated Energy Systems (IES) connect various energy systems closer, which has become an important energy utilization mode in the process of energy transition. Because the complex and variable multiple load is an important part of the new power system, the load forecasting is of great significance for the planning, operation, control, and dispatching of the new power system. In order to timely track the latest research progress of the load forecasting method and grasp the current research hotspot and the direction of load forecasting, this paper reviews the relevant research content of the forecasting methods. Firstly, a brief overview of Integrated Energy Systems and load forecasting is provided. Secondly, traditional forecasting methods based on statistical analysis and intelligent forecasting methods based on machine learning are discussed in two directions to analyze the advantages, disadvantages, and applicability of different methods. Then, the results of Integrated Energy Systemss multiple load forecasting for the past 5 years are compiled and analyzed. Finally, the Integrated Energy Systems load forecasting is summarized and looked forward.
A fast-partitioning decision method for demand side resources based on grid resilience assessment
Authors: Kong Yueping , Yang Shihai , Duan Meimei , Zhou Yuqi , Ding Zecheng , Zhang Tingquan , Sheng Ju
Published: November 2023

With the large-scale renewable energy integrated into the distribution grid, the grid’s regulating ability and disturbance tolerance are weakening. When partitioning demand-side resources, it is necessary to enhance resilience to ensure the reliability of electric power. This paper proposes a fast-partitioning method that considers resilience, structure, and functionality to adapt to the evolving requirements of the distribution system. Specifically, the comprehensive partition index system is constructed with the resilience assessment index reflecting the ability of partitions to withstand and mitigate the effects of faults, the modularity index based on electrical distance, and regional power balance indexes. Meanwhile, a modified genetic algorithm is proposed to calculate the comprehensive partition index. The modified algorithm first uses a sensitivity matrix to perform initial partitions and construct initial populations. Then, it utilizes a triangular network adjacency matrix for chromosome encoding, significantly reducing the algorithm’s search space and enhancing partitioning efficiency. Finally, the applicability and effectiveness of the proposed method are verified through simulation analysis of the IEEE 28-node system.
Predicted changes in habitat suitability for human schistosomiasis intermediate host snails for modelled future climatic conditions in KwaZulu-Natal, South Africa
Authors: Nwoko Onyekachi Esther , Manyangadze Tawanda , Chimbari Moses John
Published: November 2023

Introduction: Climate change alters environmental and climatic conditions, leading to expansion or contraction and possible shifts in the geographical distribution of vectors that transmit diseases. Bulinus globosus and Biomphalaria pfeifferi are the intermediate host snails for human schistosomiasis in KwaZulu-Natal (KZN) province, South Africa.

Methods: Using the Maximum entropy (MaxEnt) model, we modelled the current and future distribution of human schistosomiasis intermediate host snails in KZN using two representation concentration pathways (RCP4.5 and RCP8.5) for the year 2085. Thirteen and ten bioclimatic variables from AFRICLIM were used to model the habitat suitability for B. globosus and B. pfeifferi, respectively. The Jack-knife test was used to evaluate the importance of each bioclimatic variable.

Results: Mean temperature warmest quarter (BIO10, 37.6%), the number of dry months (dm, 32.6%), mean diurnal range in temperature (BIO2, 10.8%), isothermality (BIO3, 6.7%) were identified as the top four bioclimatic variables with significant contribution to the model for predicting the habitat suitability for B. globosus. Annual moisture index (mi, 34%), mean temperature warmest quarter (BIO10, 21.5%), isothermality (BIO3, 20.5%), and number of dry months (dm, 7%) were identified as the four important variables for the habitat suitability of B. pfeifferi. Area under the curve for the receiving operating characteristics was used to evaluate the performance of the model. The MaxEnt model obtained high AUC values of 0.791 and 0.896 for B. globosus and B. pfeifferi, respectively. Possible changes in the habitat suitability for B. globosus and B. pfeifferi were observed in the maps developed, indicating shrinkage and shifts in the habitat suitability of B. pfeifferi as 65.1% and 59.7% of the current suitable habitats may become unsuitable in the future under RCP4.5 and RCP8.5 climate scenarios. Conversely, an expansion in suitable habitats for B. globosus was predicted to be 32.4% and 69.3% under RCP4.5 and RCP8.5 climate scenarios, with some currently unsuitable habitats becoming suitable in the future.

Discussion: These habitat suitability predictions for human schistosomiasis intermediate host snails in KZN can be used as a reference for implementing long-term effective preventive and control strategies for schistosomiasis.
Simulation and prediction of land use in urban agglomerations based on the PLUS model: a case study of the Pearl River Delta, China
Authors: Gong Jing , Du Hongyan , Sun Yong , Zhan Yun
Published: November 2023

The Pearl River Delta (PRD) is a highly urbanized region in China that faces significant challenges in land use management. These challenges include the decrease in agricultural and ecological land resulting from rapid urbanization, the effectiveness of government governance, and the trajectory of development, all of which warrant careful research examination. Moreover, existing studies on land use in the PRD predominantly rely on static historical analysis, lacking a multi-scenario simulation approach. This study examines land use in PRD using a Patch-Generating Simulation (PLUS), from 1985 to 2020 to address this gap. Three scenarios were established to simulate potential land use outcomes in the PRD by 2030: spontaneous change, cropland protection, and ecological protection. The findings reveal that cropland, forest, and impervious surfaces are the dominant land use types in the PRD. From 1985 to 2020, the proportion of cropland decreased from 37.63% to 27.40%, with most conversions occurring to impervious surfaces and forest land. The proportion of impervious surfaces increased from 1.81% to 12.57%, primarily from conversions of cropland, forest, and water bodies. Economic development, population growth, accessibility, climatic factors, and topographic conditions were shown to be the primary determinants of land use in the PRD. Modelling results suggest that under the spontaneous change scenario, cropland and ecological land decrease, while impervious surfaces expand significantly, threatening cropland preservation and ecological construction. However, under the cropland protection scenario, the conversion rate of cropland to other land types can be effectively controlled, contributing to efficient preservation. Under the ecological protection scenario, impervious infrastructure encroachment on ecological land can be mitigated, but cropland protection is limited. The study proposes cropland protection and ecological priority policies to optimize the structure of land use, enhance efficiency, and offer policy guidance for the efficient utilization of land resources and the preservation of the ecological environment in the PRD.
Carbon sequestration and soil nitrogen enrichment in Robinia pseudoacacia L. post-mining restoration plantations
Authors: Xanthopoulos Giorgos , Radoglou Kalliopi , Derrien Delphine , Spyroglou Gavriil , Angeli Nicolas , Tsioni Georgia , Fotelli Mariangela N.
Published: November 2023

Robinia pseudoacacia L. (black locust) has been extensively used for restoring degraded lands, following anthropogenic interventions like coal mining. Here we have addressed the contribution of black locust restoration plantations, established on overburden post-mining material, to carbon storage and to soil nitrogen enrichment at the largest lignite center in Greece. Carbon stocks and fluxes in all pools of the ecosystem, as well as the foliar nitrogen resorption efficiency and soil N stocks were quantified and the effect of plantations’ age was tested. The young age of the plantations (4–24 years) resulted in a relatively low total ecosystem C stock (56.7 t ha−1), which was partitioned among the different pools in the following order: above-ground biomass (50%) > black locust-derived SOC (24%) > coarse roots (14%) > deadwood (6%) > forest floor (5%) > fine roots (less than 1%). Litterfall started early in the growing season and together with fine roots that had a turnover rate of 0.62 yr−1, fueled soil organic carbon. SOC accrual, referring to the accumulation of SOC derived by black locust, declined with age. However, further SOC accumulation is expected, based on the potential SOC storage capacity of soil at the area. C stocks in above- and below-ground biomass increased linearly with age. The same response was observed for soil N stock and NRE, indicating that despite the N2-fixing capacity of black locust, there was still a poor pedospheric N supply and a need for efficient N cycling. Overall, the studied restoration plantations have a considerable contribution to C and N accumulation at the degraded post-mining sites. These positive effects are expected to further increase at least until the plantations reach maturity.
Workforce planning in project-driven companies: a high-level guideline
Authors: Micheli G. J. L. , Martino A. , Porta F. , Cravello A. , Panaro M. , Calabrese A.
Published: November 2023

Workforce Planning (WFP) has become a crucial part of the governance of project-driven companies and has been deemed fundamental to drive critical decisions on resource management. To manage manpower planning, companies independently developed internal procedures according to their sector, size, and skills. Despite the efforts to create a reliable workforce planning process, a lack of knowledge, standardization and sharing might lead to misalignment and to heterogeneous approaches among different organizations. This study aims at investigating the current knowledge of the WFP, pointing at the detection of its key factors in terms of process steps, application context, methods, input data, actors, tools and reports’ frequency. Additionally, it attempts to define WFP high-level guidelines which can be generally valid for project-driven organizations. The research seeks to meet these goals by combining the results of the academic literature review on the WFP with the findings of the empirical study in which the representatives of ten project-based enterprises participated. The paper describes the key principles of WFP and its main process’ sections, offering high-level guidelines in terms of recommended process steps, actors involved, operative models, data input, report’s frequency, and tools. The presented features, generated by the literature review and the empirical study, are meant to be generally applicable to project-driven companies and to support the practitioners initiating this process in their organization.
Numerical models reveal the suitable habitat area for the stock enhancement and release of marine organism larvae in Liaodong Bay
Authors: Hu Xiaowei , Hou Wenhao , Sheng Zhaojun , Xi Yanbin , Yu Jiaxuan , Zhang Ruijin
Published: November 2023

Stock enhancement can effectively increase population sustainablility and improve fishery resources, making it crucial to discern the suitable habitats for stock enhancement based on efficiency considerations. In this paper, a comprehensive model was established to simulate environmental characteristics in the Liaodong Bay. A habitat suitability model was developed by considering the optimal growth conditions of the Portunus trituberculatus larvae (PTL). The coupled model showed that the optimal area for stock-enhancement with PTL occurs in late June, and the initial suitable habitat area identified represents 17.12% of the whole Liaodong Bay. Based on the larval migration model of PTL, the deviation between the larvae and the suitable habitat, as well as the actual available area for stock enhancement, were further determined after larval release in the initial suitable habitat. Only 33.67% of the larvae fulfilled the criteria of remaining within the suitable habitat for 95% of the time, and these larvae represented 6.19% of the whole area of Liaodong Bay. These findings means that the truly area available for stock enhancement is likely to be a very small portion of the entire bay, and more precise release of larvae will be necessary to ensure survival rates after release. Our study actually provides a methodological framework for the identification of suitable environment of stock enhancement. This methodology can provide technical guidance for the stock enhancement of marine larvae with same applicability for other bays, which in turn contributes to the sustainable use of marine ecosystem services and fisheries resources.
Long-term morphological and sedimentological changes caused by bottom trawling on the northern Catalan continental shelf (NW Mediterranean)
Authors: Durán Ruth , Puig Pere , Paradis Sarah , Guillén Jorge , Palanques Albert , Lo Iacono Claudio , Arjona-Camas Marta , Muñoz Araceli , Micallef Aaron
Published: November 2023

Bottom trawling is a fishing method that involves towing of nets along the seafloor to catch demersal species. The dragging of trawling gears along the seafloor results in scraping and ploughing of the surficial sediments, leading to the formation of turbid plumes of resuspended sediments and causing measurable changes in the seabed morphology. High-resolution multibeam data, side scan sonar, sediment grain size and vessel tracking data have been used to investigate the impact of bottom trawling on the seafloor morphology and surficial sediments of the northern Catalan continental shelf (NW Mediterranean), providing new insights into the impact of this anthropogenic activity on the seafloor. Multibeam data evidenced the occurrence of large-scale erosive features as a consequence of repeated scouring by fishing gears in localized areas. They are characterized by elongated (70-300 m wide and up to 8 km long) channelized areas of high backscatter with variable incision (from 0.2 m to 1.2 m). The spatial distribution of these morphologies shows a similar pattern to that observed in the fishing intensity, with maximum values in the areas of increased trawling intensity, corresponding to the main fishing grounds. Side scan sonar data also shows higher densities of trawl marks in these areas than in the surroundings. Sediment cores collected on these features show an upward-coarsening trend in the first 4-5 cm of the core, suggesting that part of the finer fraction resuspended by trawling is winnowed, increasing the sand content of the surface sediment. The identification of such large erosive morphologies in the main fishing grounds evidences that repeated trawling over the same fishing ground during decades can result in deep excavation of the seafloor, leading to permanent large-scale morphological changes. Furthermore, the evolution of these erosive features over a 13-year interval points towards long recovery periods after the cessation of trawling activities.
Non-deterministic reef fish community assembly in an upwelling-influenced transitional subprovince of the southwestern Atlantic
Authors: Carnaúba Emily A. A. , Gomes Lorena de M. J. , Garcia Gabriel S. , Cordeiro Cesar A. M. M. , Mendes Thiago C. , Ferreira Carlos E. L. , Gherardi Douglas F. M.
Published: November 2023

There are two major theories for setting up ecological communities, the Niche Theory and the Neutral Theory. Both seek to explain the main factors that form a community, which is a great challenge, since each community has its particularities and the environment has different ways to manifest. We devised a process-oriented study that sought to establish the role of environmental niche driven by coastal upwelling in the assembly of reef fish communities from exposed and sheltered environments a few kilometers apart, in the region of Arraial do Cabo (southwestern Atlantic). A multivariate hierarchical generalized linear mixed model fitted with Bayesian inference was applied to abundance and presence-absence data from visual census, together with environmental data from satellite and reanalysis. We found a stronger contribution of random effects to abundance variance with 24% for sites and 20.7% for sheltered vs. exposed locations, and weaker environmental effects with 7.1% for surface chlorophyll-a concentration (SCC) and 5.4% for sea surface temperature (SST). Environmental effects had a stronger contribution in the presence-absence model, with 20.1% for SCC and 14.6% for SST. The overall influence of the upwelling environment across all species was negative, e.g., Gymnothorax moringa and Canthigaster figueiredoi showing negative responses to SCC and Parablennius pilicornis and Malacoctenus delalandii to SST. The joint action of migration-niche mechanisms is inferred from the dominance of spatio-temporal structure, limited influence of life history traits and phylogeny, explaining around 95% of species niches in the abundance model. Our results bring new evidence for the importance of different filters for community assembly other than the environment, such as phylogenetic history and dispersal. We also discuss the balance between niche (environment) and neutral (stochasticity) processes for the assembly of reef fish communities in a tropical-subtropical transition zone.
Internal wave activity in the deep Gulf of Mexico
Authors: Meunier Thomas , Le Boyer Arnaud , Molodtsov Sergey , Bower Amy , Furey Heather , Robbins Pelle
Published: November 2023

Internal wave activity in the Gulf of Mexico (GoM) is investigated using a fleet of profiling floats. The floats continuously measured temperature and salinity as they drifted at a parking depth of 1500 dbar, allowing for the reconstruction of 2615 time series of isopycnal displacements. Thanks to the dense sampling of the eastern part of the GoM (east of 90°W), the geographical distribution of the internal waves displacement variance and available potential energy (APE) is revealed. The Loop Current (LC) influence region, between the Yucatan shelf to the west and the southern West Florida shelf to the east exhibits increased displacement variance and APE both in the continuum and near-inertial bands, while the north-eastern and central GoM show reduced internal wave activity. As the LC position fluctuates between a retracted and extended mode, we assessed the impact of the presence or absence of the LC in the increased internal wave activity region. It is shown that in the LC influence region, APE is increased (decreased) when the LC is present (absent), suggesting a strong control of the LC on deep internal waves activity. The 1500 dbar flow velocity, bottom roughness, and float altitude also seem to contribute to increased internal waves APE, but their influence is more subtle. Oppositely, no correlation with wind speed or wind intermittency is found.
Development of clinical trials for non-small cell lung cancer drugs in China from 2005 to 2023
Authors: Jia Wanying , Yu Haiyan , Song Li , Wang Jian , Niu Shuyu , Zang Guojie , Liang Mingjie , Liu Jinwei , Na Risu
Published: November 2023

Objective

Over the past few decades, the development of anti-cancer drugs in China has made outstanding achievements based on the support of national policies. To assess the progress of non-small cell lung cancer (NSCLC) drugs, we conducted a statistical analysis of clinical trials of drugs targeting NSCLC in China from 2005 to 2023.

Methods

We downloaded, screened and analysed the data from three official websites, the Centre for Drug Evaluation of China National Medical Products Administration website (NMPA), ClinicalTrials.gov and the Chinese Clinical Trial Registry (ChiCTR).

Results

From January 1, 2005 to April 15, 2023, a total of 1,357 drug clinical trials that met the standards were included, and the number of registered drug clinical trials has been increasing year by year, reaching the maximum of 199 in 2021. Among them, the maximum of 462 items (34.05%) in phase II clinical trials, followed by 333 (24.54%) in phase III clinical trials, and 139 (10.24%) in phase IV clinical trials. In all drug clinical trials, industry sponsored trials (ISTs) have 722 items (53.21%), which are higher than investigator-initiated trials (IITs). The clinical trials of chemical drugs have a maximum of 723 items (53.28%), while biopharmaceuticals have grown rapidly in the past 10 years, with a total of 374 (27.56%), and 48.19% of the drug clinical trials of combined medication. In addition, the geographical distribution of the leading units and participating units of Chinese drug clinical trials are uneven, and economic regions such as Beijing, Shanghai, Jiangsu are obviously ahead of other regions.

Conclusion

From 2005 to 2023, the clinical trials of registered drugs for the treatment of NSCLC increased rapidly. Among them, due to the development of immunotherapy, the clinical trials of biopharmaceuticals and drugs for combined medication are growing most rapidly, while the exploration of the original drugs is a little far from enough. Our research provides a direction for the future drug clinical trials of NSCLC, laying foundation for further extending the survival rate of patients with NSCLC.
Artificial intelligence in rheumatoid arthritis: potential applications and future implications
Authors: Gilvaz Vinit J. , Reginato Anthony M.
Published: November 2023

The widespread adoption of digital health records, coupled with the rise of advanced diagnostic testing, has resulted in an explosion of patient data, comparable in scope to genomic datasets. This vast information repository offers significant potential for improving patient outcomes and decision-making, provided one can extract meaningful insights from it. This is where artificial intelligence (AI) tools like machine learning (ML) and deep learning come into play, helping us leverage these enormous datasets to predict outcomes and make informed decisions. AI models can be trained to analyze and interpret patient data, including physician notes, laboratory testing, and imaging, to aid in the management of patients with rheumatic diseases. As one of the most common autoimmune diseases, rheumatoid arthritis (RA) has attracted considerable attention, particularly concerning the evolution of diagnostic techniques and therapeutic interventions. Our aim is to underscore those areas where AI, according to recent research, demonstrates promising potential to enhance the management of patients with RA.
A domesticated photoautotrophic microbial community as a biofilm model system for analyzing the influence of plastic surfaces on invertebrate grazers in limnic environments
Authors: Bakenhus Insa , Jongsma Rense , Michler-Kozma Diana , Hölscher Lea , Gabel Friederike , Holert Johannes , Philipp Bodo
Published: November 2023

The environmental fate of plastic particles in water bodies is influenced by microbial biofilm formation. Invertebrate grazers may be affected when foraging biofilms on plastics compared to biofilms on natural substrata but the mechanistic basis for these effects is unknown. For analyzing these effects in ecotoxicological assays stable and reproducible biofilm communities are required that are related to the environmental site of interest. Here, a defined biofilm community was established and used to perform grazing experiments with a freshwater snail. For this, snippets of different plastic materials were incubated in the photic zone of three different freshwater sites. Amplicon sequencing of biofilms formed on these snippets showed that the site of incubation and not the plastic material dominated the microbial community composition. From these biofilms, individual microbial strains as well as photoautotrophic consortia were isolated; these consortia consisted of heterotrophic bacteria that were apparently nourished by microalga. While biofilms formed by defined dual cultures of a microalga and an Alphaproteobacterium were not accepted by the snail P. fontinalis, a photoautotrophic consortium (Co_3) sustained growth and metabolism of this grazer. Amplicon sequencing revealed that consortium Co_3, which could be stably maintained on solid medium under photoautotrophic conditions, reproducibly formed biofilms of a defined composition on three different plastic materials and on glass surfaces. In conclusion, our study shows that the generation of domesticated photoautotrophic microbial communities is a valid novel approach for establishing laboratory ecotoxicological assays with higher environmental relevance than those based on defined microbiota.
Adaptive laboratory evolution of Salmonella enterica in acid stress
Authors: Ghoshal Mrinalini , Bechtel Tyler D. , Gibbons John G. , McLandsborough Lynne
Published: November 2023

Introduction

Adaptive laboratory evolution (ALE) studies play a crucial role in understanding the adaptation and evolution of different bacterial species. In this study, we have investigated the adaptation and evolution of Salmonella enterica serovar Enteritidis to acetic acid using ALE.

Materials and methods

Acetic acid concentrations below the minimum inhibitory concentration (sub-MIC) were used. Four evolutionary lineages (EL), namely, EL1, EL2, EL3, and EL4, of S. Enteritidis were developed, each demonstrating varying levels of resistance to acetic acid.

Results

The acetic acid MIC of EL1 remained constant at 27 mM throughout 70 days, while the MIC of EL2, EL3, and EL4 increased throughout the 70 days. EL4 was adapted to the highest concentration of acetic acid (30 mM) and demonstrated the highest increase in its MIC against acetic acid throughout the study, reaching an MIC of 35 mM on day 70. The growth rates of the evolved lineages increased over time and were dependent on the concentration of acetic acid used during the evolutionary process. EL4 had the greatest increase in growth rate, reaching 0.33 (h−1) after 70 days in the presence of 30 mM acetic acid as compared to EL1, which had a growth rate of 0.2 (h−1) after 70 days with no exposure to acetic acid. Long-term exposure to acetic acid led to an increased MIC of human antibiotics such as ciprofloxacin and meropenem against the S. enterica evolutionary lineages. The MIC of ciprofloxacin for EL1 stayed constant at 0.016 throughout the 70 days while that of EL4 increased to 0.047. Bacterial whole genome sequencing revealed single-nucleotide polymorphisms in the ELs in various genes known to be involved in S. enterica virulence, pathogenesis, and stress response including phoP, phoQ, and fhuA. We also observed genome deletions in some of the ELs as compared to the wild-type S. Enteritidis which may have contributed to the bacterial acid adaptation.

Discussion

This study highlights the potential for bacterial adaptation and evolution under environmental stress and underscores the importance of understanding the development of cross resistance to antibiotics in S. enterica populations. This study serves to enhance our understanding of the pathogenicity and survival strategies of S. enterica under acetic acid stress.
A closer look to neural pathways and psychopharmacology of obsessive compulsive disorder
Authors: Gargano Steven P. , Santos Melody G. , Taylor Sydney M. , Pastis Irene
Published: November 2023

The intricate neural pathways involved in obsessive-compulsive disorder (OCD) affect areas of our brain that control executive functioning, organization, and planning. OCD is a chronic condition that can be debilitating, afflicting millions of people worldwide. The lifetime prevalence of OCD in the US is 2.3%. OCD is predominantly characterized by obsessions consisting of intrusive and unwanted thoughts, often with impulses that are strongly associated with anxiety. Compulsions with OCD encompass repetitive behaviors or mental acts to satisfy their afflicted obsessions or impulses. While these factors can be unique to each individual, it has been widely established that the etiology of OCD is complex as it relates to neuronal pathways, psychopharmacology, and brain chemistry involved and warrants further exploration.
Glial regulation of critical period plasticity
Authors: Starkey Jacob , Horstick Eric J. , Ackerman Sarah D.
Published: November 2023

Animal behavior, from simple to complex, is dependent on the faithful wiring of neurons into functional neural circuits. Neural circuits undergo dramatic experience-dependent remodeling during brief developmental windows called critical periods. Environmental experience during critical periods of plasticity produces sustained changes to circuit function and behavior. Precocious critical period closure is linked to autism spectrum disorders, whereas extended synaptic remodeling is thought to underlie circuit dysfunction in schizophrenia. Thus, resolving the mechanisms that instruct critical period timing is important to our understanding of neurodevelopmental disorders. Control of critical period timing is modulated by neuron-intrinsic cues, yet recent data suggest that some determinants are derived from neighboring glial cells (astrocytes, microglia, and oligodendrocytes). As glia make up 50% of the human brain, understanding how these diverse cells communicate with neurons and with each other to sculpt neural plasticity, especially during specialized critical periods, is essential to our fundamental understanding of circuit development and maintenance.
Longer duration entry mitigates nystagmus and vertigo in 7-Tesla MRI
Authors: Pogson Jacob M. , Shemesh Ari , Roberts Dale C. , Zee David S. , Otero-Milan Jorge , Ward Bryan K.
Published: November 2023

Introduction

Patients and technologists commonly describe vertigo, dizziness, and imbalance near high-field magnets, e.g., 7-Tesla (T) magnetic resonance imaging (MRI) scanners. We sought a simple way to alleviate vertigo and dizziness in high-field MRI scanners by applying the understanding of the mechanisms behind magnetic vestibular stimulation and the innate characteristics of vestibular adaptation.

Methods

We first created a three-dimensional (3D) control systems model of the direct and indirect vestibulo-ocular reflex (VOR) pathways, including adaptation mechanisms. The goal was to develop a paradigm for human participants undergoing a 7T MRI scan to optimize the speed and acceleration of entry into and exit from the MRI bore to minimize unwanted vertigo. We then applied this paradigm from the model by recording 3D binocular eye movements (horizontal, vertical, and torsion) and the subjective experience of eight normal individuals within a 7T MRI. The independent variables were the duration of entry into and exit from the MRI bore, the time inside the MRI bore, and the magnetic field strength; the dependent variables were nystagmus slow-phase eye velocity (SPV) and the sensation of vertigo.

Results

In the model, when the participant was exposed to a linearly increasing magnetic field strength, the per-peak (after entry into the MRI bore) and post-peak (after exiting the MRI bore) responses of nystagmus SPV were reduced with increasing duration of entry and exit, respectively. There was a greater effect on the per-peak response. The entry/exit duration and peak response were inversely related, and the nystagmus was decreased the most with the 5-min duration paradigm (the longest duration modeled). The experimental nystagmus pattern of the eight normal participants matched the model, with increasing entry duration having the strongest effect on the per-peak response of nystagmus SPV. Similarly, all participants described less vertigo with the longer duration entries.

Conclusion

Increasing the duration of entry into and exit out of a 7T MRI scanner reduced or eliminated vertigo symptoms and reduced nystagmus peak SPV. Model simulations suggest that central processes of vestibular adaptation account for these effects. Therefore, 2-min entry and 20-s exit durations are a practical solution to mitigate vertigo and other discomforting symptoms associated with undergoing 7T MRI scans. In principle, these findings also apply to different magnet strengths.
Abnormal functional connectivity of the core olfactory network in patients with chronic rhinosinusitis accompanied by olfactory dysfunction
Authors: Ma Yao , Jiang Jian , Wu Ying , Xiong Jiaxin , Lv Huiting , Li Jiahao , Kuang Hongmei , Jiang Xiaofeng , Chen Yeyuan
Published: November 2023

Objective

To review and analyze the functional connectivity (FC) abnormalities in the brain olfactory network (ON) of patients with chronic rhinosinusitis with olfactory dysfunction (CRSwOD) and explore the relationship between these FC abnormalities and olfactory dysfunction, providing clues to the neurophysiological mechanisms underlying CRSwOD.

Methods

FC analysis on the ON of patients with CRSwOD and patients with chronic rhinosinusitis without olfactory dysfunction (CRSsOD) identified the regions of the ON with abnormal FC in CRSwOD patients, and the correlation between abnormal FC and clinical scales for chronic rhinosinusitis was analyzed.

Results

(1) Compared with the CRSsOD group, CRSwOD patients showed decreased FC between the bilateral orbitofrontal cortex (OFC) and the right middle frontal gyrus, (2) Receiver operating characteristic (ROC) curve analysis revealed that the FC value between the right middle frontal gyrus and the left OFC (area under the curve (AUC) = 0.852, sensitivity: 0.821, specificity: 0.800, p < 0.001) was more capable of distinguishing whether CRS patients may have olfactory dysfunction than the FC value between the right middle frontal gyrus and the right OFC (AUC = 0.827, sensitivity: 0.893, specificity: 0.667, p < 0.001), and (3) Lund-Kennedy scores were positively correlated with the FC values between the right middle frontal gyrus and the left OFC (r = 0.443, p < 0.018). Lund-Mackay scores were also positively correlated with the FC values between the right middle frontal gyrus and the left OFC (r = 0.468, p < 0.012). Questionnaire of Olfactory Disorders-Negative Statements scores were negatively correlated with the FC values between the right middle frontal gyrus and the left OFC (r = −0.481, p < 0.001).

Conclusion

Persistent nasal inflammation affects the FC between the middle frontal gyrus and the OFC, which may serve as a potential imaging marker for identifying CRSwOD. The severity of nasal inflammation and olfactory damage is closely related to the FC between the middle frontal gyrus and OFC, and the abnormal changes in this FC can be used to explain the neurophysiological mechanisms behind the occurrence of olfactory dysfunction in patients.