bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Performance Properties of an Aerospace Epoxy Resin Loaded with Carbon Nanofibers and Glycidyl Polyhedral Oligomeric Silsesquioxane
Authors: Guadagno Liberata , Pantelakis Spiros , Strohmayer Andreas , Raimondo Marialuigia
Published: April 2022

This paper proposes a new multifunctional flame retardant carbon nanofiber/glycidyl polyhedral oligomeric silsesquioxane (GPOSS) epoxy formulation specially designed for lightweight composite materials capable of fulfilling the ever-changing demands of the future aerospace industry. The multifunctional resin was designed to satisfy structural and functional requirements. In particular, this paper explores the advantages deriving from the combined use of GPOSS and CNFs (short carbon nanofibers) to obtain multifunctional resins. The multifunctional material was prepared by incorporating in the epoxy matrix heat-treated carbon nanofibers (CNFs) at the percentage of 0.5 wt% and GPOSS compound at 5 wt% in order to increase the mechanical performance, electrical conductivity, thermal stability and flame resistance property of the resulting nanocomposite. Dynamic mechanical analysis (DMA) shows that the values of the Storage Modulus (S.M.) of the resin alone and the resin containing solubilized GPOSS nanocages are almost similar in a wide range of temperatures (from 30 °C to 165 °C). The presence of CNFs, in the percentage of 0.5 wt%, determines an enhancement in the S.M. of 700 MPa from −30 °C to 180 °C with respect to the resin matrix and the resin/GPOSS systems. Hence, a value higher than 2700 MPa is detected from 30 °C to 110 °C. Furthermore, the electrical conductivity of the sample containing both GPOSS and CNFs reaches the value of 1.35 × 10−1 S/m, which is a very satisfying value to contrast the electrical insulating property of the epoxy systems. For the first time, TUNA tests have been performed on the formulation where the advantages of GPOSS and CNFs are combined. TUNA investigation highlights an electrically conductive network well distributed in the sample. The ignition time of the multifunctional nanocomposite is higher than that of the sample containing GPOSS alone of about 35%.
Seedling Establishment and Yield Performance of Dry Direct-Seeded Rice after Wheat Straw Returning Coupled with Early Nitrogen Application
Authors: Tian Jinyu , Li Shaoping , Xing Zhipeng , Cheng Shuang , Liu Qiuyuan , Zhou Lei , Liao Ping , Hu Yajie , Guo Baowei , Wei Haiyan , Zhang Hongcheng
Published: April 2022

Dry direct-seeded rice sown by multifunctional seeders (MS-DDSR) has received increased attention for its high efficiency. Wheat straw returning is widely used as an important agricultural practice because it is the simplest and quickest approach to dispose of wheat straw and also improve soil quality. The study determined whether MS-DDSR after wheat straw returning could obtain a high yield and whether early nitrogen (N) application could compensate for the negative effects caused by returned wheat straw. Field experiments were performed in a split-plot design. Main plots were comprised without wheat straw returning (S0) and wheat straw returning (S1). Split plots consisted of three plots with early N application treatment: 65 (N1), 95 (N2), and 125 (N3) kg N ha−1 at 0 and 20 days after sowing. S1 reduced yield, N uptake, and biomass accumulation in MS-DDSR compared to S0 because S1 negatively affected the seedling roots growth, seedling establishment, and tillering capacity of MS-DDSR. The positive interaction between wheat straw returning and early N on yield, biomass accumulation, and N uptake was likely related to the positive interaction on spikelet number per panicle, total spikelet number, and biomass accumulation after the stem elongation stage. These findings demonstrate that wheat straw returning led to poor seedling establishment and yield loss for MS-DDSR, but these negative effects could be compensated for by an appropriate increase in early N application, based on the locally recommended N application protocols.
Dynamics of Pyricularia oryzae Population Race Structures from 2003 to 2017 in Jiangsu Province, China
Authors: Qi Zhongqiang , Du Yan , Liu Muxing , Yu Junjie , Zhang Rongsheng , Yu Mingna , Cao Huijuan , Song Tianqiao , Pan Xiayan , Liang Dong , Liu Yongfeng
Published: April 2022

Rice blast, caused by Pyricularia oryzae, is one of the most destructive rice diseases worldwide. It is essential to understand the population structure and race distribution of P. oryzae for the prevention and control of rice blast. This study collected 1584 isolates across Jiangsu province from 2003 to 2017 to determine race diversity, common and dominant race structures, and resistance profiles using eight parameters from the Chinese differential cultivars (CDC) entries. Race diversity analysis indicated that the race diversity of 1584 isolates in Jiangsu province increased from 2003 to 2006 and fluctuated steadily from 2007 to 2017. Common race structure analysis showed that the common race structure of the isolates fluctuated wildly on an annual basis. Moreover, the race ZG1 was the dominant race, suggesting that the most commonly grown rice varieties in Jiangsu are highly adaptive to race ZG1. In addition, due to a higher level (>85%) of resistance to the population of isolates from 2003 to 2017, Tetep may be conformed as the most promising donor of blast resistance for resistance breeding in Jiangsu province. In summary, it is beneficial to control rice blast by continuous monitoring of the population structure and distribution of P. oryzae with the monogenic germplasm entries and by maintaining a diversity of rice varieties.
Characterization of Source–Sink Traits and Carbon Translocation in Maize Hybrids under High Plant Density
Authors: Ren Hong , Qi Hua , Zhao Ming , Zhou Wenbin , Wang Xinbing , Gong Xiangwei , Jiang Ying , Li Congfeng
Published: April 2022

Both compact planting and selecting superior maize (Zea mays L.) hybrids can greatly optimize the source–sink relationship and enhance maize productivity. However, the underlying physiological mechanism for regulating carbon (C) assimilate transport and influencing grain yield between maize cultivars has remained unclear under contrasting plant densities. A two-year field experiment was conducted to investigate grain yield, vascular bundle character, grain filling, C allocation in grains and other tissues, and hormone level and enzyme activity in grains under 60,000 (ND) and 90,000 plants ha−1 (HD) densities using Xianyu 335 (XY335) and Zhengdan958 (ZD958) hybrids. Compared to the ZD958, XY335 increased grain yield, kernel number per plant (KNP), and sink capacity by 11.4%, 15.7%, and 7.4%, respectively. Moreover, XY335 performed higher net photosynthetic rate and sucrose synthase activities in grains than those in ZD958, and higher levels of sucrose phosphate synthase and soluble acid invertase activity were mainly exhibited in the middle of the grain filling stage, which contributed to increasing the proportion of grain in total dry matter, grain C content and leaf C transport efficiency by 4.3%, 12.2%, and 52.9%, respectively, under HD conditions. Additionally, a greater area and number of small vascular bundle in ear of XY335 resulted in 21.3% higher matter transport efficiency and 4.8% higher maximum grain filling rate than ZD958 under HD conditions. In addition, grains of XY335 exhibited generally higher levels of indole acetic acid (IAA) and abscisic acid (ABA), as well as ABA/GA3 ratio after maize pollination relative to those from ZD958, conducive to regulating C translocation from leaves to grains. Overall, our study illustrates that stronger source activity, sink characteristics, and matter transport channels for maize hybrids are significant for C assimilate transport to grain for achieving high grain yield under higher plant density.
Pumpkin Yield Estimation Using Images from a UAV
Authors: Midtiby Henrik Skov , Pastucha Elżbieta
Published: April 2022

The paper presents a pumpkin yield estimation method using images acquired by a UAV. The processing pipeline is fully automated. It consists of orthomosaic generation, a color model collection using a random subset of the data, color segmentation, and finally counting of pumpkin blobs together with assessing the number of pumpkins in each blob. The algorithm was validated by a manual check of 5% of each tested dataset. The precision value ranges between 0.959 and 0.996, recall between 0.971 and 0.987, and F1 score falls between 0.971 and 0.988. This proves the very high efficiency of the processing workflow and its potential value to farmers.
Evaluation of Combining Ability and Heterosis of Popular Restorer and Male Sterile Lines for the Development of Superior Rice Hybrids
Authors: Azad Abul Kalam , Sarker Umakanta , Ercisli Sezai , Assouguem Amine , Ullah Riaz , Almeer Rafa , Sayed Amany A. , Peluso Ilaria
Published: April 2022

Twenty-four hybrids, obtained from a mating design following 6 line × 4 testers, were evaluated to estimate the heterosis, specific, and general combining ability (SCA and GCA) of parents and hybrids to find out suitable general combiner (GC) parents and cross combinations for utilization in the future breeding program. A randomized complete block design with three replications was followed to set the experiment. Data were recorded on grain yield and 13 yield-related agronomic traits. The analysis of variance of all cross combinations had highly significant differences for most of the characters studied, which indicated a wide variation across the genotypes, parents, lines, testers, and crosses. SCA and GCA variances were significant for all studied traits except for the panicle length, indicating that both non-additive and additive gene actions were involved in these traits. The GCA variance/SCA variance for all the traits was <1, signifying the multitude of dominant and epistatic gene actions. The GCA effects of three lines GAN46A, IR58025A, IR62629A, and a tester IR46R were significant for the majority of the agronomic traits including grain yield and might be used for improving the yield of grains in rice as parents of excellent GC. Based on the yield of grains and agronomic traits, the hybrids IR58025A × IR46R and GAN46A × IR46R might be considered the best hybrids and another nine hybrids could also be considered good hybrids. Similarly, based on the yield of grains and agronomic traits, the positive and significant mid-parent, better parent, and standard heterosis were obtained from 3 F1s, 1 F1, and 3 F1s, respectively. Heterosis and combining ability study revealed that hybrids IR58025A × IR46R and GAN46A × IR46R might be considered preferable hybrid cultivars.
The Relationship between Soil Respiration and Plant Community Functional Traits in Ebinur Lake Basin
Authors: Sheng Huiyi , Wang Jinlong , He Xuemin , Lv Guanghui
Published: April 2022

Soil respiration (Rs) plays an important role in the carbon cycle of terrestrial ecosystems. Understanding the impacts of plant community functional traits on Rs is a key prerequisite for accurate prediction of the future carbon balance of terrestrial ecosystems under climate change. This study examined the relationship of Rs with plants in the Ebinur Lake Basin in the arid desert region. Traditional statistical methods and geostatistical methods were used to study the spatial variation characteristics of Rs and to analyze the effects of plant community functional traits and environmental factors on the spatial heterogeneity of Rs. The variation in Rs in the arid desert area of the Ebinur Lake Basin showed a strong spatial dependence (C0/(C + C0) = 0.11) and a medium variation (α = 25.50, CV = 86.21%). Principal component analysis revealed that morphological traits of plants and soil water content had great contributions to PC1, soil nutrient had great contributions to PC2, and physiological traits of plants and soil temperature had large contributions to PC3. Multiple regression analysis showed that PC1, PC2, and PC3 can explain 83% of the spatial heterogeneity of Rs (F = 157.41, p < 0.01). In conclusion, maximum height, leaf width, leaf dry matter content, leaf thickness, and soil water content were the principal driving elements of soil respiration heterogeneity.
Behavioral Assessment of Six Reptile Species during a Temporary Zoo Closure and Reopening
Authors: Hamilton Jennifer , Gartland Kylen N. , Jones Megan , Fuller Grace
Published: April 2022

Although reptiles are commonly housed in zoos and aquariums, their welfare is understudied for the diversity of species housed and the taxon’s current captive population size. The sensory abilities of reptiles have adapted to the varied ecological niches they inhabit, and these evolutionary adaptations impact how reptiles perceive the stimuli around them—including zoo visitors. This study aimed to assess visitor effects on small groups of six reptile species during a temporary zoo closure due to COVID-19 by measuring behavioral diversity, use of space (measured by a spread of participation index), and select behaviors. The species assessed showed diverse responses. The Catalina Island rattlesnakes (Crotalus catalinensis) demonstrated increased investigation and behavioral diversity after the zoo reopened compared to when the zoo was closed, but the European glass lizards (Pseudopus apodus) showed decreases in the amount of time spent exposed to the observers’ view and in their evenness of space use after the zoo was reopened to visitors. The other species, including beaded lizards (Heloderma horridum), Sonoran spiny-tailed iguana (Ctenosaura macrolopha), Arrau turtles (Podocnemis expansa), and dwarf caimans (Paleosuchus palpebrosus), had intermediate changes in their responses to visitor presence.
Growth Performance, Rumen Fermentation and Inflammatory Response on Holstein Growing Cattle Treated with Low and High Non-Fibrous Carbohydrate to Neutral Detergent Fiber Ratio Pelleted Total Mixed Ration
Authors: Chen Yinyin , Gong Xiaoxiao , Huang Yinghao , Jiang Maocheng , Zhan Kang , Lin Miao , Zhao Guoqi
Published: April 2022

Twenty-four healthy castrated male Holstein growing cattle, with similar body weight (301 ± 11.5 kg), were enrolled in this study and randomly allocated into two groups (12/pen). Holstein growing cattle in the LPT (low NFC/NDF pelleted TMR) group were fed basal pelleted TMR with a low NFC/NDF ratio (NFC/NDF = 1.07), while the HPT (high NFC/NDF pelleted TMR) group were fed with a high NFC/NDF ratio diet (NFC/NDF = 1.71). The results showed that: (1) Body measurements were found to be increased for the LPT group (p < 0.05); compared with the HPT group, feed intake to gain ratio and feed cost in the LPT group were decreased by 12.24% and 15.35%, respectively (p < 0.01). Compared with the HPT group, the LPT group tended to increase chest girth. (2) Digestibility of DM and NDF in the LPT group was higher (p < 0.05) than in the HPT group, being increased by 3.41% and 4.26%, respectively, and increased digestibility of ADF in the LPT group was significant (p < 0.01). (3) The daily feed consumption of NDF in the LPT group was higher than that in the HPT group and the daily rumination time and chewing time in the LPT group were longer than that in the HPT group (p < 0.05). (4) Compared with the LPT group, the parameter of pH, microbial protein and acetate: propionate (p < 0.05) in the HPT group were decreased by 8.57%, 12.46% and 23.71%, respectively. In contrast, the concentration of total volatile fatty acids, acetate and propionate were higher (p < 0.05) in the HPT group, and increased by 13.49%, 19.59% and 52.70%, respectively. (5) Compared with the LPT group, rumen fluid in the HPT group diet up-regulated the mRNA expression levels of BRECs pro-inflammatory factor IL-1β and TNF-α (p < 0.05), and meanwhile, up-regulated the mRNA expression levels of BRECs pro-inflammatory factor IL-6 (p < 0.01); compared with the LPT group, rumen fluid in the HPT group diet up-regulated the mRNA expression levels of CCL28 and CCL20 (p < 0.05) chemokines in CCL types of BRECs; in addition, compared with the LPT group, rumen fluid in the HPT group up-regulated the mRNA expression levels of CXCL2, CXCL3, CXCL9 and CXCL14 chemokines in CXCL types of BRECs (p < 0.01), and the mRNA expression levels of the CXCL5 chemokine tended to be increased (p = 0.06).
Fermentation Quality, In Vitro Digestibility, and Aerobic Stability of Total Mixed Ration Silage in Response to Varying Proportion Alfalfa Silage
Authors: Xie Yixiao , Wang Lei , Li Wenqi , Xu Shengyang , Bao Jinze , Deng Jiajie , Wu Zhe , Yu Zhu
Published: April 2022

This study aimed to evaluate the effects of different proportions of alfalfa silage on the fermentation quality, in vitro digestibility, and aerobic stability of total mixed ration (TMR) silage. Three TMRs were prepared with different silage contents on a fresh matter basis: (1) 60% alfalfa silage (AS60), (2) 40% alfalfa silage (AS40), and (3) 20% alfalfa silage (AS20). The lactic acid in AS60 did not increase after 30 days of ensiling (p > 0.05). Butyric acid was detected in the AS20 group after 14 days of ensiling. The AS60 group showed significantly higher in vitro dry matter digestibility than the AS20 group (p < 0.05). The aerobic stability of TMR silage gradually increased with a decreasing percentage of alfalfa silage (p < 0.05). Unlike AS60, which directly gained an acidic environment from the alfalfa silage, AS40 developed a stable acidic environment during ensiling and further improved aerobic stability. However, when the percentage of alfalfa silage was reduced to 20%, a risk of clostridial spoilage occurred in the TMR silage. Therefore, the addition of 40% alfalfa silage to TMR is optimal and could achieve both good fermentation quality and considerable resistance to aerobic deterioration in TMR silage.
Special Issue “Antibody Engineering for Cancer Immunotherapy”
Authors: Crescioli Silvia , White Ann L. , Karagiannis Sophia N.
Published: April 2022

Since the approval of Rituximab in the late 1990s, the first chimeric monoclonal antibody for the treatment of non-Hodgkin lymphoma, antibody engineering for cancer immunotherapy has become a rapidly growing field, with almost 50 antibody therapeutics approved in the USA and EU and hundreds undergoing testing in clinical trials [...]
Current Knowledge on Biomaterials for Orthopedic Applications Modified to Reduce Bacterial Adhesive Ability
Authors: Allizond Valeria , Comini Sara , Cuffini Anna Maria , Banche Giuliana
Published: April 2022

A significant challenge in orthopedics is the design of biomaterial devices that are able to perform biological functions by substituting or repairing various tissues and controlling bone repair when required. This review presents an overview of the current state of our recent research into biomaterial modifications to reduce bacterial adhesive ability, compared with previous reviews and excellent research papers, but it is not intended to be exhaustive. In particular, we investigated biomaterials for replacement, such as metallic materials (titanium and titanium alloys) and polymers (ultra-high-molecular-weight polyethylene), and biomaterials for regeneration, such as poly(ε-caprolactone) and calcium phosphates as composites. Biomaterials have been designed, developed, and characterized to define surface/bulk features; they have also been subjected to bacterial adhesion assays to verify their potential capability to counteract infections. The addition of metal ions (e.g., silver), natural antimicrobial compounds (e.g., essential oils), or antioxidant agents (e.g., vitamin E) to different biomaterials conferred strong antibacterial properties and anti-adhesive features, improving their capability to counteract prosthetic joint infections and biofilm formation, which are important issues in orthopedic surgery. The complexity of biological materials is still far from being reached by materials science through the development of sophisticated biomaterials. However, close interdisciplinary work by materials scientists, engineers, microbiologists, chemists, physicists, and orthopedic surgeons is indeed necessary to modify the structures of biomaterials in order to achieve implant integration and tissue regeneration while avoiding microbial contamination.
Wild Duck (Anas platyrhynchos) as a Source of Antibiotic-Resistant Salmonella enterica subsp. diarizonae O58—The First Report in Poland
Authors: Pławińska-Czarnak Joanna , Wódz Karolina , Piechowicz Lidia , Tokarska-Pietrzak Ewa , Bełkot Zbigniew , Bogdan Janusz , Wiśniewski Jan , Kwieciński Piotr , Kwieciński Adam , Anusz Krzysztof
Published: April 2022

The “One Health” approach increasingly demonstrates the global spread of pathogenic microorganisms and their antimicrobial resistance in the environment, both in animals and humans. Salmonella enterica subsp. diarizonae is nowadays very often isolated from cold-blooded reptiles to a lesser extent from sheep, but unfortunately more and more often from humans. However, there are a few studies describing the isolation of Salmonella enterica subsp. diarizonae from migratory wild birds. The mallard duck (Anas platyrhynchos), a wild animal that traverses the continent of Eurasia, can be an excellent indicator of the spread of intestinal microbes as well as their resistance to antibiotics. This is the first report of the Salmonella enterica subsp. diarizonae detection in Poland in a migrating mallard duck. This research presented the identification difficulties associated with the isolation of Salmonella enterica subsp. diarizonae using three different biochemical tests and advanced serology tests. At the same time, we detected very high antimicrobial resistance in the isolated strain. By using the minimum inhibitory concentration (MIC) method, it was found that the isolated strain of S. enterica subsp. diarizonae has high antibiotic resistance against 14 of the 33 tested antimicrobials agents. The resistance genes that have been identified in S. enterica subsp. diarizonae include aadA, strA/strB, and blaTEM.
Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves
Authors: Goulart Debora Brito , Beyi Ashenafi Feyisa , Wu Zuowei , Adiguzel Mehmet Cemal , Schroeder Anastasia , Singh Kritika , Xu Changyun , Ocal Melda Meral , Dewell Renee , Dewell Grant A. , Plummer Paul J. , Zhang Qijing , Sahin Orhan
Published: April 2022

Campylobacter is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) Campylobacter in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in C. jejuni, 30 commercial calves were divided into Group 1, Group 2, and Group 3 (n = 10), and were all inoculated orally with FQ-susceptible (FQ-S) C. jejuni; seven days later, Group 3 was challenged with transtracheal Mannheimia haemolytica, and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R Campylobacter via culture. Before oral inoculation with C. jejuni, 87% of calves were naturally colonized by FQ-R C. jejuni. Two days after the inoculation, FQ-R C. jejuni decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all C. jejuni populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R C. jejuni populations following the injection was due mainly to enrichment of preexisting FQ-R C. jejuni, rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant Campylobacter in cattle in response to FQ treatment.
Antibiotic Resistance of Salmonella Typhimurium Monophasic Variant 1,4,[5],12:i:-in China: A Systematic Review and Meta-Analysis
Authors: Qin Xiaojie , Yang Mingzhe , Cai Hua , Liu Yangtai , Gorris Leon , Aslam Muhammad Zohaib , Jia Kai , Sun Tianmei , Wang Xiang , Dong Qingli
Published: April 2022

Antibiotic resistance in Salmonella is a global public health problem. Salmonella enterica serovar 1,4,[5],12:i:- (S. 1,4,[5],12:i:-), a monophasic variant of Salmonella Typhmurium, is one of the leading Salmonella serovars in several countries. This study aimed to assess the prevalence of antibiotic resistance to this serovar in China through a systematic review and meta-analysis. Nineteen eligible studies during 2011–2021 were included. A total of 4514 isolates from humans, animals, foods, and the environment were reported, which mainly concerned isolates found in Guangdong, Guangxi, Jiangsu, and Shanghai. A random-effects model was used to estimate the pooled resistance rate of S. 1,4,[5],12:i:-. Rates were found to be very high (values ≥ 75%) for tetracycline, ampicillin, sulfisoxazole, and streptomycin; high (50–75%) for nalidixic acid, amoxicillin–clavulanic acid, and chloramphenicol; and moderate (25–50%) for trimethoprim–sulfamethoxazole, kanamycin, trimethoprim, and gentamicin. The rates of resistance to ciprofloxacin, cefotaxime, ceftriaxone, cefepime, ceftazidime, and colistin were low (values ≤ 25%), but of great concern in terms of their current clinical importance. Furthermore, a high multidrug resistance rate (86%, 95% CI: 78–92%) was present in S. 1,4,[5],12:i:-, with the ASSuT pattern largely dominating. Subgroup analysis results showed that the high heterogeneity of resistance rates was not entirely dependent on isolated sources. Taken together, the severity of antibiotic resistance in S. 1,4,[5],12:i:- urgently requires the rational use of antibiotics in future infection control and antibiotic stewardship programs.
Role of Oxidative Stress in Diabetic Cardiomyopathy
Authors: De Geest Bart , Mishra Mudit
Published: April 2022

Type 2 diabetes is a redox disease. Oxidative stress and chronic inflammation induce a switch of metabolic homeostatic set points, leading to glucose intolerance. Several diabetes-specific mechanisms contribute to prominent oxidative distress in the heart, resulting in the development of diabetic cardiomyopathy. Mitochondrial overproduction of reactive oxygen species in diabetic subjects is not only caused by intracellular hyperglycemia in the microvasculature but is also the result of increased fatty oxidation and lipotoxicity in cardiomyocytes. Mitochondrial overproduction of superoxide anion radicals induces, via inhibition of glyceraldehyde 3-phosphate dehydrogenase, an increased polyol pathway flux, increased formation of advanced glycation end-products (AGE) and activation of the receptor for AGE (RAGE), activation of protein kinase C isoforms, and an increased hexosamine pathway flux. These pathways not only directly contribute to diabetic cardiomyopathy but are themselves a source of additional reactive oxygen species. Reactive oxygen species and oxidative distress lead to cell dysfunction and cellular injury not only via protein oxidation, lipid peroxidation, DNA damage, and oxidative changes in microRNAs but also via activation of stress-sensitive pathways and redox regulation. Investigations in animal models of diabetic cardiomyopathy have consistently demonstrated that increased expression of the primary antioxidant enzymes attenuates myocardial pathology and improves cardiac function.
In Silico Identification of Novel Inhibitors Targeting the Homodimeric Interface of Superoxide Dismutase from the Dental Pathogen Streptococcus mutans
Authors: Cerchia Carmen , Roscetto Emanuela , Nasso Rosarita , Catania Maria Rosaria , De Vendittis Emmanuele , Lavecchia Antonio , Masullo Mariorosario , Rullo Rosario
Published: April 2022

The microaerophile Streptococcus mutans, the main microaerophile responsible for the development of dental plaque, has a single cambialistic superoxide dismutase (SmSOD) for its protection against reactive oxygen species. In order to discover novel inhibitors of SmSOD, possibly interfering with the biofilm formation by this pathogen, a virtual screening study was realised using the available 3D-structure of SmSOD. Among the selected molecules, compound ALS-31 was capable of inhibiting SmSOD with an IC50 value of 159 µM. Its inhibition power was affected by the Fe/Mn ratio in the active site of SmSOD. Furthermore, ALS-31 also inhibited the activity of other SODs. Gel-filtration of SmSOD in the presence of ALS-31 showed that the compound provoked the dissociation of the SmSOD homodimer in two monomers, thus compromising the catalytic activity of the enzyme. A docking model, showing the binding mode of ALS-31 at the dimer interface of SmSOD, is presented. Cell viability of the fibroblast cell line BJ5-ta was not affected up to 100 µM ALS-31. A preliminary lead optimization program allowed the identification of one derivative, ALS-31-9, endowed with a 2.5-fold improved inhibition power. Interestingly, below this concentration, planktonic growth and biofilm formation of S. mutans cultures were inhibited by ALS-31, and even more by its derivative, thus opening the perspective of future drug design studies to fight against dental caries.
Rosemary Extracts Improved the Antioxidant Status of Low-Fat Yoghurt Sauces Enriched with Inulin
Authors: Martínez-Tomé Magdalena , Cedeño-Pinos Cristina , Bañón Sancho , Jiménez-Monreal Antonia M.
Published: April 2022

Yoghurt sauces are considered fatty products which are quite susceptible to oxidation and must be stabilised using antioxidants. Novel formulations for yoghurt sauces often involve replacement of fat with dietary fibres and use of natural preservatives. The aim of the present research was to design healthier formulations for yoghurt sauces based on the replacement of sunflower oil (SO) with chicory inulin (IN) and the use of rosemary extracts (RE) as natural antioxidants. Different sauces were developed by adding IN at 2 and 5% w: w and/or 300 mg/kg lipo- and/or water-soluble rosemary extracts (RLE and/or RWE) containing 120 and 146 mg polyphenols per g extract, respectively. Nutritional value (proximate composition and caloric contribution), some physical properties (pH and CIELab colour) and antioxidant status (deoxyribose, DPPH radical scavenging, Rancimat, lipid peroxidation and linoleic acid assays) were assessed in the sauces. Replacement of SO with IN (5%) reduced fat content by 30%, roughly 15% low calories, thereby obtaining healthier sauces. As expected, the RLE was more effective than the RWE in improving antioxidant activity in lipidic environment. Using RLE enhanced the antioxidant capacity of lipid peroxidation by 44%. In the Rancimat test, this increased the oxidative protection of the sauce made with and without IN (5%) by around 20% or 45%, respectively. Similarly, using RLE doubled protection against linoleic acid oxidation. Application of IN in yoghurt sauce has nutritional (replacement of lipids with dietary fibre) and technological interest (foaming agent) and can be combined with RE of high polyphenol content as a potential functional ingredient capable of stabilising the sauces against oxidation.
The Structure Research and Design for Beam Steering and Adjustment in Golay3 Sparse-Aperture Imaging System
Authors: Qian Junhong , Wu Xiaoyan , Liu Hewei , Hua Xiubao , Tao Ye , Zhang Rongzhu
Published: April 2022

In order to further explore the technical difficulties involved in orienting the sparse-aperture imaging system towards practical applications, this paper proposes an optical–mechanical structure design scheme and performance simulation, as well as actual alignment on the beam steering and adjustment structure, which is the core component of the Golay3 sparse-aperture imaging system. The beam steering and adjustment structure corrects the cophasing error by using a combination of mechanical rough adjustment and piezoelectric ceramic precision adjustment. The beam adjustment capability of the beam steering and adjustment structure is analyzed by simulation when the system contains the piston error and the tilt error. The piston error is controlled within 12 μm and the tilt error is controlled within 1200 μrad through the mechanical rough adjustment light path using a large-diameter collimation as the point light source, which realizes the confocality of three beams and mutual interference between two beams. With the USAF1951 resolution test panel display in the distance as the surface target, the adjustable piezoelectric ceramics control the piston error within 55 nm and the tilt error within 0.25 μrad, obtaining the surface target imaging result of the sparse aperture.
Effect of Mass Transport by Convective Flow on the Distribution of Dissolved Carbon Monoxide in a Stirred Tank
Authors: Lim Youngwook , Min Junwon , Kang Taewook , Kim Dongchoul
Published: April 2022

The dissolved gas concentration in a stirred tank has significant importance in the chemical and biological processing industries as mass transfer from the injected gas to an aqueous solution must occur for the gas to be usable. As the solubility of the gas in the solution is low and there are no probes for measuring dissolved gas concentration, a volumetric mass transfer coefficient is selected as a criterion of design for the scale-up of stirred reactors. However, it is difficult to accurately predict the non-equilibrium state dissolved gas distribution using only the volumetric mass transfer coefficient. In this study, computational fluid dynamics (CFD)-based numerical analysis was conducted to systematically evaluated the effects of mass transport by convective flow on the distribution of dissolved carbon monoxide in a stirred tank. The dissolved carbon monoxide distribution and the volumetric mass transfer coefficient were compared at various rotational speeds of the impellers. At a rotational speed of 900 RPM, the Pearson correlation coefficient was about 0.52, which denotes a moderate correlation. In contrast, Pearson correlation coefficients less than 0.20 were obtained for speeds less than 700 RPM, indicating a weak correlation. By considering the dissolved carbon monoxide transport that occurs during convective flow in stirred tanks, we can provide more accurate information about the dissolved carbon monoxide distribution.
Investigation of Time-Varying Cable Tension of Bridges Using Time-Frequency Reassignment Techniques Based on Structural Health Monitoring Data
Authors: Cao Maosen , Hu Shuaitao , Zhang Xin , Zhang Shixiang , Sumarac Dragoslav , Peng Jiayi
Published: April 2022

Cables have been increasingly utilized in modern long-span or tied-arch bridges as the main bearing structures. Real-time identification of time-varying cable tension is essential for assessing the service performance of bridges. Vibration-based methods have been an increasing research focus in recent decades. However, a long time interval is needed to estimate structural frequency using vibration-based methods, increasing the calculating time of cable tension. The time-varying cable tension is thus difficult to extract. This study proposes a time-frequency reassignment-based algorithm to reduce the detection time to address this issue. Combined with a time-frequency analysis tool and vibration theory of cables, the algorithm can identify the time-varying frequency and further quickly calculate the time-varying cable tension within 12.8 s. The features of the proposed algorithm are mainly threefold: identifying the time-varying frequencies with high precision; without some prior knowledge of vibration; having no other requirements for sensor modes. Moreover, the experimental validation is conducted using a quasi-static loading in a workshop and a dynamic field test on Sutong Bridge, respectively. The results show that the proposed algorithm can be used to identify time-varying tension and assess the service performance of cables, providing a new path for real-time condition monitoring of bridges in service.
Breakup Dynamics of Droplets in Symmetric Y-Junction Microchannels
Authors: Lei Li , Zhao Yuting , An Jun , Zhang Bo , Zhang Jingzhi
Published: April 2022

The experimental method is used to study the droplet breaking characteristics of an immiscible liquid–liquid t8wo-phase fluid in symmetric Y-junction microchannels. Silicone oil is used as the dispersed phase and distilled water containing 0.5% SDS is used as the continuous phase. Three breakup behaviors were observed: breakup with permanent obstruction, breakup with gaps, and no breakup. Two stages of the change of the neck width of the sub-droplet during the breakup process were discovered: a rapid breakup stage and a thread breakup stage. The effect of the breakup behavior on the flow pattern was investigated and it was found that the breakup behavior of the droplets made the slug flow area smaller; further, a new flow pattern was observed, being droplet flow. The length of the sub-droplet increases with an increase of the volume flow rate of the dispersed phase and the ratio of the volume flow rate of the dispersed phase to the continuous phase, while decreasing with an increase of the volume flow rate and the capillary number of the continuous phase. Based on the influence of the two-phase flow parameters on the length of the sub-droplet, a correlation formula for the length of the sub-droplet with good predictive performance is proposed.
The Application of Satellite Image Analysis in Oil Spill Detection
Authors: Tysiąc Paweł , Strelets Tatiana , Tuszyńska Weronika
Published: April 2022

In recent years, there has been an increasing use of satellite sensors to detect and track oil spills. The satellite bands, namely visible, short, medium infrared, and microwave radar bands, are used for this purpose. The use of satellite images is extremely valuable for oil spill analysis. With satellite images, we can identify the source of leakage and assess the extent of potential damage. However, it is not yet clear how to approach a specific leakage case methodologically. The aim of this study is the remote sensing analysis of environmental changes with the development of oil spill detection processing methods. Innovative elements of the work, in addition to methodological proposals, include the long-term analysis of surface water changes. This is very important because oil is very likely to enter the soil when water levels change. The classification result was satisfactory and accurate by 85%. The study was carried out using images from Landsat 5, Landsat 7, Landsat 8, Sentinel-1, and Sentinel-2 satellites. The results of the classification of the oil stains in active and passive technologies differ. This difference affects the methodology for selecting processing methods in similar fields. In the case of this article, the oil spill that occurred on 29 May 2020 in Norilsk was investigated and compared with data from other years to determine the extent of biodegradation. Due to the tank failure that occurred at the Nornickel power plant on that day, a large amount of crude oil leaked into the environment, contaminating the waters and soil of local areas. Research shows that oil spills may be caused by human error or may be the effect of climate change, particularly global warming.
Influence of Freeze-Thaw Aging on the Impact Performance of Damped Carbon Fiber Reinforced Plastics for Automotive Applications
Authors: Virgillito Enrico , Sisca Lorenzo , Carello Massimiliana
Published: April 2022

The increasing use of composite materials in the automotive field requires more attention with regards to the appearance of noise, vibration and harshness (NVH) study in cars construction. However, in car door panels production, impact characteristics need to be evaluated in sandwich laminates. Furthermore, it is important to consider the effect of prolonged environmental aging on crashworthiness properties. The innovative content of the work is the hygrothermal effects evaluation on impact performance for two damped CFRP sandwich laminates. In this paper, two damping materials, Kraibon HHZ9578/99 and SUT9609/24, were used as core between two skins of CFRP for sandwich composite production. Freeze-Thaw aging treatment according to IEC 60068, specific for Automotive, was performed to investigate environmental effects on components. Up to 750 h, it was demonstrated that water absorption is regulated by Fick’s Law. The low-velocity impact behavior of the damped sandwiches has been studied according to ASTM D7136 throughout drop dart test equipment. Both main peak forces and energy absorption characteristics are negatively affected by aging condition. The introduction of damping core inside the composite structure of vehicle components can satisfy NVH constrictions. By contrast, at least same operating conditions must be assured in relation to not-damped components.
Pose Measurement and Motion Estimation of Space On-Orbit CubeSats Based on Micro-Doppler Effect Using Laser Coherent Radar
Authors: Zhang Yong , Han Yi , Yu Ruonan , Yang Zhen , Wang Zhengjia , Zhang Jianlong
Published: April 2022

The pose measurement and motion estimation of long-distance space CubeSats is a difficult problem for autonomous rendezvous and track maintenance. In this work, an approach is proposed for the pose estimation of CubeSat based on the micro-Doppler effect by a laser radar system. Parameters such as spin rate and attitude angle can be estimated using data extracted from the returned signal. By utilizing threshold processing, edge extraction of time-frequency (TF) images can be achieved effectively. When the target size is unknown, the double edge ratio method (DERM) is suggested to obtain the attitude angle. The simulation results show that when the noise is not too high, the spin rate and the attitude angle can be acquired precisely. Our experiment indicates that the error for the estimation of spin and pose by our approach fulfills the demand in practice.
On Training Data Selection in Condition Monitoring Applications—Case Azimuth Thrusters
Authors: Nikula Riku-Pekka , Ruusunen Mika , Böhme Stephan André
Published: April 2022

Machine learning techniques are commonly used in the vibration-based condition monitoring of rotating machines. However, few research studies have focused on model training from a practical viewpoint, namely, how to select representative training samples and operating areas for monitoring applications. We focus on these aspects by studying training sets with varying sizes and distributions, including their effects on the models to be identified. The analysis is based on acceleration and shaft speed data available from an azimuth thruster of a catamaran crane vessel. The considered machine learning algorithm was previously introduced in another study suggesting it could detect defects on the thruster driveline components. In this work, practical guidance is provided to facilitate its implementation, and furthermore, an adaptive method for training subset selection is proposed. Results show that the proposed method enabled the identification of usable training subsets in general, while the success of the previous approach was case-dependent. In addition, the use of Kolmogorov–Smirnov or Anderson–Darling tests for normal distribution, as a part of the method, enabled selections that covered the operating area broadly, while other tests were unfavorable in this regard. Overall, the study demonstrates that reconfigurable and automated model implementations could be achievable with minor effort.
Experimental Study on Frost Crystal Morphologies and Frosting Characteristics under Different Working Pressures
Authors: Lai Tianwei , Liu Xue , Dong Xiaojun , Qiang Mingchen , Yan Shaohang , Hou Yu
Published: April 2022

Frost usually grows on the cooling surface of heat transfer equipment, which often operates under different working pressures. In such conditions, one of the fundamental influencing factors of frosting, the diffusion coefficient of water vapor around the cooling surface, is different from that in the normal atmosphere pressure. In order to investigate the pressure effect on frosting, a test rig for frosting visualization is designed. The role of working pressure around the cooling surface on the frost crystal morphologies and frost layer characteristics is evaluated under different air parameters and temperatures of cooling surface. The vertical growth and lateral distribution of frost crystals are strongly related to working pressure around the cooling surface. More frost crystal morphologies and faster growth rate of frost layer are presented at a lower pressure. The frost thickness, frost accumulation and density reduce with higher pressure. The dimensionless correlation of frost thickness is proposed considering working pressure around the cooling surface, humid air parameters, and temperature of cooling surface.
Optimal Multikey Homomorphic Encryption with Steganography Approach for Multimedia Security in Internet of Everything Environment
Authors: Abunadi Ibrahim , Abdullah Mengash Hanan , S. Alotaibi Saud , Asiri Mashael M. , Ahmed Hamza Manar , Zamani Abu Sarwar , Motwakel Abdelwahed , Yaseen Ishfaq
Published: April 2022

Recent developments of semiconductor and communication technologies have resulted in the interconnection of numerous devices in offering seamless communication and services, which is termed as Internet of Everything (IoE). It is a subset of Internet of Things (IoT) which finds helpful in several applications namely smart city, smart home, precise agriculture, healthcare, logistics, etc. Despite the benefits of IoE, it is limited to processing and storage abilities, resulting in the degradation of device safety, privacy, and efficiency. Security and privacy become major concerns in the transmission of multimedia data over the IoE network. Encryption and image steganography is considered effective solutions to accomplish secure data transmission in the IoE environment. For resolving the limitations of the existing works, this article proposes an optimal multikey homomorphic encryption with steganography approach for multimedia security (OMKHES-MS) technique in the IoE environment. Primarily, singular value decomposition (SVD) model is applied for the separation of cover images into RGB elements. Besides, optimum pixel selection process is carried out using coyote optimization algorithm (COA). At the same time, the encryption of secret images is performed using poor and rich optimization (PRO) with multikey homomorphic encryption (MKHE) technique. Finally, the cipher image is embedded into the chosen pixel values of the cover image to generate stego image. For assessing the better outcomes of the OMKHES-MS model, a wide range of experiments were carried out. The extensive comparative analysis reported the supremacy of the proposed model over the rennet approaches interms of different measures.
Development of a Radio-Frequency Quadrupole Accelerator for the HL-2A/2M Tokamak Diagnostic System
Authors: Liu Shuo , Wei Yaxia , Lu Yuanrong , Wang Zhi , Han Meiyun , Wei Tianhao , Xia Yin , Li Haipeng , Gao Shuli , Zheng Pengfei
Published: April 2022

In order to figure out the migration and deposition of impurities on the first wall of HL-2A/2M tokamak, Peking University and Southwestern Institute of Physics are co-developing a deuteron RFQ as part of the in situ ion-beam diagnostic for the material. The RFQ, which operates at 162.5 MHz, is designed to accelerate a 10-mA deuteron beam from 40 keV up to 1.5 MeV. Key design considerations and the final design parameters are presented. The RFQ has been conditioned at a 1% duty factor for 80 h at RF cavity power of 55 kW. The specific shunt impedance of the cavity is 221 kΩ·m by measuring the bremsstrahlung spectrum. The intrinsic Q-value after the high-power tests measured by the Ring-Down method is 13,780. Beam commissioning has been taken place during the first half of 2021, and the beam measurements include beam current and energy of 2H+ ion. A 10 mA 2H+ beam was successfully accelerated through the RFQ.