bluuebunny/crossref_metadata_2025 huggingface dataset:
Physics-informed neural networks for solving time-dependent mode-resolved phonon Boltzmann transport equation
Authors: Zhou Jiahang , Li Ruiyang , Luo Tengfei
Published: November 2023

The phonon Boltzmann transport equation (BTE) is a powerful tool for modeling and understanding micro-/nanoscale thermal transport in solids, where Fourier’s law can fail due to non-diffusive effect when the characteristic length/time is comparable to the phonon mean free path/relaxation time. However, numerically solving phonon BTE can be computationally costly due to its high dimensionality, especially when considering mode-resolved phonon properties and time dependency. In this work, we demonstrate the effectiveness of physics-informed neural networks (PINNs) in solving time-dependent mode-resolved phonon BTE. The PINNs are trained by minimizing the residual of the governing equations, and boundary/initial conditions to predict phonon energy distributions, without the need for any labeled training data. The results obtained using the PINN framework demonstrate excellent agreement with analytical and numerical solutions. Moreover, after offline training, the PINNs can be utilized for online evaluation of transient heat conduction, providing instantaneous results, such as temperature distribution. It is worth noting that the training can be carried out in a parametric setting, allowing the trained model to predict phonon transport in arbitrary values in the parameter space, such as the characteristic length. This efficient and accurate method makes it a promising tool for practical applications such as the thermal management design of microelectronics.
Chromosome-scale genomes of commercially important mahoganies, Swietenia macrophylla and Khaya senegalensis
Authors: Sahu Sunil Kumar , Liu Min , Wang Guanlong , Chen Yewen , Li Ruirui , Fang Dongming , Sahu Durgesh Nandini , Mu Weixue , Wei Jinpu , Liu Jie , Zhao Yuxian , Zhang Shouzhou , Lisby Michael , Liu Xin , Xu Xun , Li Laigeng , Wang Sibo , Liu Huan , He Chengzhong
Published: November 2023

Mahogany species (family Meliaceae) are highly valued for their aesthetic and durable wood. Despite their economic and ecological importance, genomic resources for mahogany species are limited, hindering genetic improvement and conservation efforts. Here we perform chromosome-scale genome assemblies of two commercially important mahogany species: Swietenia macrophylla and Khaya senegalensis. By combining 10X sequencing and Hi-C data, we assemble high-quality genomes of 274.49 Mb (S. macrophylla) and 406.50 Mb (K. senegalensis), with scaffold N50 lengths of 8.51 Mb and 7.85 Mb, respectively. A total of 99.38% and 98.05% of the assembled sequences are anchored to 28 pseudo-chromosomes in S. macrophylla and K. senegalensis, respectively. We predict 34,129 and 31,908 protein-coding genes in S. macrophylla and K. senegalensis, respectively, of which 97.44% and 98.49% are functionally annotated. The chromosome-scale genome assemblies of these mahogany species could serve as a vital genetic resource, especially in understanding the properties of non-model woody plants. These high-quality genomes could support the development of molecular markers for breeding programs, conservation efforts, and the sustainable management of these valuable forest resources.
High-throughput density functional theory screening of double transition metal MXene precursors
Authors: Nykiel Kat , Strachan Alejandro
Published: November 2023

MXenes are an emerging class of 2D materials of interest in applications ranging from energy storage to electromagnetic shielding. MXenes are synthesized by selective etching of layered bulk MAX phases into sheets of 2D MXenes. Their chemical tunability has been significantly expanded with the successful synthesis of double transition metal MXenes. While knowledge of the structure and energetics of double transition metal MAX phases is critical to designing and optimizing new MXenes, only a small subset of these materials been explored. We present a comprehensive dataset of key properties of MAX phases obtained using density functional theory within the generalized gradient approximation exchange-correlation functionals. Energetics and structure of 8,712 MAX phases have been calculated and stored in a queryable, open database hosted at nanoHUB.
Contribution of ammonia-oxidizing archaea and bacteria to nitrogen transformation in a soil fertilized with urea and organic amendments
Authors: Yang Yajun , Liu Hexiang , Zhang Yang , Fang Xianhui , Zhong Xianbao , Lv Jialong
Published: November 2023

The contribution of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) is crucial for nitrogen transformation. The effects of four organic amendments (OAs) plus urea on soil nitrogen transformation and the contribution of the ammonia-oxidizing microbial community were investigated using an incubation experiment. The OAs plus urea treatments included pig manure plus urea (PM + U), wheat straw plus urea (WS + U), compost plus urea (CP + U) and improved-compost plus urea (IC + U), while no OAs and urea amended control was noted as CK. The abundance and composition of AOA and AOB were determined using high through-put sequencing. Compared with CK, the OA plus urea treatments significantly enhanced the amount of total mineralized nitrogen released during the incubation process. After incubation, the highest mineralized nitrogen and net nitrogen mineralization was under the PM + U treatment and the lowest was in the WS + U treatment. In conclusion, among all OA plus urea treatments, the microbial biomass nitrogen content was the highest in WS + U treatment and dissolved organic nitrogen content was the highest with the PM + U treatment. Additionally, the abundance of AOB was inhibited in comparison to that of AOA; however, AOB contributed more to nitrification than AOA. Soil NO3−-N and dissolved organic nitrogen were the principal components influencing the distribution of AOA and AOB. The result illustrated that the OAs plus urea, especially PM plus urea promoted mineralization to produce more dissolved organic nitrogen and NH4+-N, thus accelerating the growth of AOB to strengthen nitrification in soil.
Study on environmental factors affecting the quality of codonopsis radix based on MaxEnt model and all-in-one functional factor
Authors: Wang Zixia , Jia Yanjun , Li Pengpeng , Tang Zhuoshi , Guo Yina , Wen Longxia , Yu Huaqiao , Cui Fang , Hu Fangdi
Published: November 2023

Owing to the increasing market demand of Codonopsis Radix, the cropper blindly cultivates to expand planting area for economic benefits, which seriously affects the quality of Codonopsis Radix. Therefore, this study synthesized 207 batches of Codonopsis Radix and 115 ecological factors, and analyzed the suitable planting areas of Codonopsis pilosula under current and future climate change based on Geographic Information System (GIS) and MaxEnt model. Secondly, we evaluated the quality of Codonopsis Radix based on the all-in-one functional factor including chromatographic fingerprint, the index components, the effective compounds groups, the nutritional components, and the nutritional elements, and the quality regionalization of Codonopsis Radix was analyzed. Finally, the ecological factors affecting the accumulation of effective components of Codonopsis Radix were analyzed. This study found for the first time that the highly suitable area of Codonopsis pilosula was mainly distributed in the Weihe River system and the Bailongjiang River system in Gansu Province. There were differences in the quality of Codonopsis Radix from different ecologically suitable areas based on the all-in-one functional factors, and the comprehensive high-quality area of Codonopsis Radix was mainly distributed in Longnan and Longxi district of Gansu Province. The precipitation, temperature and altitude play a key role in the accumulation of chemical components in the 10 ecological factors affecting the distribution of Codonopsis pilosula. Under future climatic conditions, the highly suitable area of Codonopsis pilosula is decreased.
Essential oil components of turmeric inhibit hepatic lipidification and liver fibrosis in a diet-induced NASH model rats
Authors: Watanabe Yukari , Watanabe Hitoshi , Kogure Sarasa , Tanioka Yuri , Yamauchi Jun , Furusho Tadasu
Published: November 2023

In this study, the fraction extracted from turmeric powder with 50% ethanol and fractionated with n-hexane were administered to diet-induced NASH model rats. NASH model was prepared with SD rats by feeding an originally designed choline-deficient, high-fat, high-fructose (HFF-CD) diet for 10 weeks. To the HFF-CD diet, hexane fraction and 50% ethanol fraction after hexane fractionation were added at 100 mg/kg body weight. 10 weeks later, blood samples and liver were collected for the following parameters: lipid weights, serum ALT, AST, TG, liver TG, TBARS levels, lipid metabolism-related gene expression and histopathological examination of the liver. As the results, the hexane fraction and 50% ethanol fraction showed a decrease in lipid weight, a decrease in hepatic TG, and activation of PPAR-α in the lipid metabolism-related gene test. These results suggest that the hexane fraction of turmeric has an inhibitory effect on fat accumulation in the liver by promoting lipid metabolism in NASH model rats.
Genome mining reveals novel biosynthetic gene clusters in entomopathogenic bacteria
Authors: Meesil Wipanee , Muangpat Paramaporn , Sitthisak Sutthirat , Rattanarojpong Triwit , Chantratita Narisara , Machado Ricardo A. R. , Shi Yi-Ming , Bode Helge B. , Vitta Apichat , Thanwisai Aunchalee
Published: November 2023

The discovery of novel bioactive compounds produced by microorganisms holds significant potential for the development of therapeutics and agrochemicals. In this study, we conducted genome mining to explore the biosynthetic potential of entomopathogenic bacteria belonging to the genera Xenorhabdus and Photorhabdus. By utilizing next-generation sequencing and bioinformatics tools, we identified novel biosynthetic gene clusters (BGCs) in the genomes of the bacteria, specifically plu00736 and plu00747. These clusters were identified as unidentified non-ribosomal peptide synthetase (NRPS) and unidentified type I polyketide synthase (T1PKS) clusters. These BGCs exhibited unique genetic architecture and encoded several putative enzymes and regulatory elements, suggesting its involvement in the synthesis of bioactive secondary metabolites. Furthermore, comparative genome analysis revealed that these BGCs were distinct from previously characterized gene clusters, indicating the potential for the production of novel compounds. Our findings highlighted the importance of genome mining as a powerful approach for the discovery of biosynthetic gene clusters and the identification of novel bioactive compounds. Further investigations involving expression studies and functional characterization of the identified BGCs will provide valuable insights into the biosynthesis and potential applications of these bioactive compounds.
Assessment of knowledge, attitude, and practices towards canine visceral leishmaniasis based on the one health concept in Weliso and Ejaji Towns, Oromia, Ethiopia
Authors: Diriba Weraka Weya , Gebremedhin Endrias Zewdu
Published: November 2023

Canine visceral leishmaniasis (CVL) is a significant vector-borne Meta zoonotic disease caused by agents of the L. donovani complex. The disease is transmitted by the bite of phlebotomine female sandflies of the genera Phlebotomus and Lutzomyia in the old and new worlds, respectively. This study was conducted to assess the knowledge, attitude, and practices of the residents about CVL based on the One Health concept in two towns of the Oromia Region, Ethiopia. A community-based cross-sectional study was conducted between October 2019 and September 2020, using an interview questionnaire as the study instrument. The study participants were selected through a simple random sampling method. Pearson’s Chi-square and logistic regression tests were used to evaluate the association between the study participants’ knowledge, attitude, and practices toward CVL and possible risk factors. The study included a total of 400 participants, and the results indicated that 77.25% had good knowledge, 60.5% had a favorable attitude, and 59.25% had good practices toward CVL. The town of Ejaji and dog ownership were significantly associated with good knowledge (p = 0.001), and attitude (p = 0.001) towards CVL, while having a dog (p = 0.001), having a diploma (p = 0.019) or degree and above (0.018), being divorced or widowed (0.012), and being Oromo (p = 0.013) were all significantly associated with good CVL practice. Most participants in both study areas had good knowledge but moderate attitudes and practices toward CVL. Therefore, it is crucial to undertake comprehensive community health education and awareness programs of zoonotic visceral leishmaniasis and its vectors based on the One Health concept through various means.
A novel SIGGW dual post band-pass filter for 5G millimeter-wave band applications with a transmission zero
Authors: Salehian Kamran , Tayarani Majid
Published: November 2023

Integration of planar circuits been considered a credible technique for low-cost mass production of microwave and millimeter-wave circuits and systems. For the first time, in this research a dual-post band-pass filter is designed and simulated in a three-layer substrate integrated gap groove waveguide (SIGGW) for 5G millimeter-wave frequency band applications. The filter includes 12 posts ($$6 \times 2$$

6
×
2

). Also, the structure facilitates to use resonant posts and so we can design the posts to add a transmission zero in lower rejection band. The design theory algorithm and its limitations are investigated based on the circuit model of filter. The results shows that FBW of 5% and a lower band transmission zero for the proposed 12 posts filter. Also, the results are verified by simulation using CST. According to the results, the proposed filter is a good option for Ka-band applications and can be used as the building block for suppressing the LO leakage that is commonly used for up-converting the 5G signal to Ka band.
Optimization of X-axis servo drive performance using PSO fuzzy control technique for double-axis dicing saw
Authors: Cao Weifeng , Zhang Peiyi , Mi Qingtao , Sun Yahui , Shi Jun , Liang Wanyong
Published: November 2023

The dicing saw is a critical piece of equipment in IC processing, primarily used to cut wafers. Due to the high spindle speed, even small errors in the cutting process can result in wafer chipping or cracking. Therefore, the dicing saw requires a high degree of accuracy and stability. In this paper, the accuracy of the X-axis servo response was simulated using an Israeli ADT-8230 dual-axis abrasive wheel dicing saw. The study introduces a novel approach by using a fuzzy controller instead of the traditional position loop proportional integral (PI) controller. In addition, a two-input, two-output fuzzy rule is used for on-line correction of the position loop PI parameters. A heuristic algorithm is used to optimise the position loop fuzzy controller parameters. The quantization and proportionality factors are rectified using Particle Swarm Optimisation (PSO) algorithm and Genetic Algorithm (GA) respectively. By comparing the performance of the PSO fuzzy and GA fuzzy controllers, the optimal control method is derived. The proposed method is validated by simulation in the MATLAB/Simulink development environment using real ADT-8230 servo data. Experimental results show that the PSO-fuzzy structured controller reduces the position control error by 11.8%, improves the tracking performance by 26% and reduces the torque pulsation by 23%. Therefore, in future research, more advanced search algorithms should be further combined to improve the servo accuracy of the dicing saw.
Plasma disinfection procedures for surfaces in emergency service vehicles: a field trial at the German Red Cross
Authors: Schaal Tom , Schmelz Ulrich
Published: November 2023

The demand for thorough disinfection within ambulances is essential, given the in-vehicle medical procedures and the potential high risk of infections due to patients' open wounds. One solution that can address this hygiene challenge involves the application of reactive products generated from atmospheric (air) oxygen and water vapor, activated through the use of cold plasma. Cold plasma's charged particles perforate the cell membranes of microorganisms. This process does not work in human cells, as proteins in the form of enzymes within the body break down the cold plasma and protect the cells. The study was done on an ambulance that was contaminated in eight places. Samples were taken from each site, and two surfaces measuring approximately 8 × 8 cm were carefully sealed and marked. These surfaces were deliberately contaminated by applying an Enterococcus faecium suspension of 8.5 × 107 CFU/mL using a sterile cotton swab. It was followed by the disinfection procedure, that was initiated with the PLASMOCAR device. It was positioned on the front workspace and operated for a duration of 30 min, utilizing the vehicle's onboard voltage. Throughout the operation, all doors and windows were closed and the vehicle's air conditioning system remained active. After the completion of the disinfection process, samples were collected from the surfaces for bacterial counts. A reduction of 3.73 log levels in initial bacteria was accomplished within the rescue vehicle for Enterococcus faecium, equivalent to a 10–fourfold reduction in bacteria, eliminating up to 99.99% of the initial microorganisms. This success makes the process well-suited and convenient as an ongoing "background" procedure to enhance the established disinfection procedures. The established disinfection procedures outlined in the hygiene plan must be promptly implemented whenever mechanical surface cleaning is required. The use of PLASMOCAR offers an extra layer of protection and security, significantly decreasing the risk of microorganism transmission through cross-contamination and aerosols. This is a significant benefit for the well-being of both staff and patients.
Manufacturing of quantum-tunneling MIM nanodiodes via rapid atmospheric CVD in terahertz band
Authors: Ozyigit Dogu , Ullah Farman , Gulsaran Ahmet , Bastug Azer Bersu , Shahin Ahmed , Musselman Kevin , Bajcsy Michal , Yavuz Mustafa
Published: November 2023

Quantum-tunneling metal–insulator-metal (MIM) diodes have emerged as a significant area of study in the field of materials science and electronics. Our previous work demonstrated the successful fabrication of these diodes using atmospheric pressure chemical vapor deposition (AP-CVD), a scalable method that surpasses traditional vacuum-based methods and allows for the fabrication of high-quality Al2O3 films with few pinholes. Here, we show that despite their extremely small size 0.002 µm2, the MIM nanodiodes demonstrate low resistance at zero bias. Moreover, we have observed a significant enhancement in resistance by six orders of magnitude compared to our prior work, Additionally, we have achieved a high responsivity of 9 AW−1, along with a theoretical terahertz cut-off frequency of 0.36 THz. Our approach provides an efficient alternative to cleanroom fabrication, opening up new opportunities for manufacturing terahertz-Band devices. The results of our study highlight the practicality and potential of our method in advancing nanoelectronics. This lays the foundation for the development of advanced quantum devices that operate at terahertz frequencies, with potential applications in telecommunications, medical imaging, and security systems.
Muscle activity variability patterns and stride to stride fluctuations of older adults are positively correlated during walking
Authors: Jordão Sofia , Stergiou Nick , Brandão Rita , Pezarat-Correia Pedro , Oliveira Raúl , Cortes Nelson , Vaz João R.
Published: November 2023

It has been found that fractal-like patterns are present in the temporal structure of the variability of healthy biological rhythms, while pathology and disease lead to their deterioration. Interestingly, it has recently been suggested that these patterns in biological rhythms are related with each other, reflecting overall health or lack of it, due to their interaction. However, the underlying neurophysiological mechanisms responsible for such dependency remain unknown. In addition, this relationship between different elements needs to be first verified before we even pursue understanding their interaction. This study aimed to investigate the relationship between two elements of the neuromuscular system, gait and muscle activity variability patterns in older adults. Twenty-one older adults walked at their preferred walking speed on a treadmill. Inter-stride intervals were obtained through an accelerometer placed on the lateral malleoli to assess the temporal structure of variability of stride-to-stride fluctuations. Inter muscle peak intervals were obtained through the electromyographic signal of the gastrocnemius to assess the temporal structure of the variability of the simultaneous muscle activity. The temporal structure of variability from both signals was evaluated through the detrended fluctuation analysis, while their magnitude of variability was evaluated using the coefficient of variation. The Pearson’s Correlation coefficient was used to identify the relationship between the two dependent variables. A significant strong positive correlation was found between the temporal structure of gait and muscle activity patterns. This result suggests that there is an interdependency between biological rhythms that compose the human neuromuscular system.
Association between mental health, psychological characteristics, and motivational functions of volunteerism among Polish and Ukrainian volunteers during the Russo-Ukrainian War
Authors: Chudzicka-Czupała Agata , Chiang Soon-Kiat , Tan Clara M. , Hapon Nadiya , Żywiołek-Szeja Marta , Karamushka Liudmyla , Paliga Mateusz , Dubniak Zlatyslav , McIntyre Roger S. , Ho Roger
Published: November 2023

The Russo-Ukrainian War has led to a humanitarian crisis, and many people volunteered to help affected refugees. This cross-sectional survey study investigates the relationships between the psychological impact of participation, coping mechanisms, and motivational functions of volunteering during the Russo-Ukrainian War among 285 Ukrainian and 435 Polish volunteers (N = 720). Multivariate linear regression was used to examine relationships between motivational functions and psychosocial and demographic characteristics. Ukrainian volunteers reported significantly higher Hyperarousal and Avoidance, Depression, Anxiety, and Stress, Problem-focused, Emotion-focused, and Avoidant coping, as well as total scores of Hardiness and Psychological Capital than Polish counterparts. Linear regression analysis found that Impact of the Event Scale results, Coping with Stress, being a female, unemployed, and religious were significantly associated with higher motivational functions. Ukrainian volunteers could significantly reduce negative feelings and strengthen social networks and religious faith by volunteering, while Polish volunteers were significantly more likely to gain skills and psychosocial development from helping others.
Impacts of COVID-19 pandemic through decomposition of life expectancy according to leading causes and place of death in Czechia
Authors: Hulíková Tesárková Klára , Dzúrová Dagmar
Published: November 2023

While the direct effects of the pandemic are well documented, less is known about the indirect ones, including changes in healthcare provision or human behavior. This paper aims to study the impact of indirect consequences on mortality, focusing on two leading causes (cardiovascular diseases, COVID-19) and places of death in Czechia, during the COVID-19 pandemic, one of the most severely affected European countries. The analysis was performed using data from the Czech Statistical Office and the Institute of Health Information and Statistics. The study compares annual mortality changes during three time periods: pre-pandemic (2018–2019), pandemic beginning and peaking (2020–2021), and pandemic fading (2022). Pandemic years were covered by the WHO public health emergency of international concern. Abridged life tables were computed, and Pollard's decomposition was used to calculate the contributions of causes and places of death on annual differences in life expectancy. Seasonal decomposition of monthly time series revealed an increase in cardiovascular mortality at home or in social care facilities corresponding to limitations in healthcare. While COVID-19 had a systemic negative effect on life expectancy during the pandemic, the impact of cardiovascular mortality according to place of death changed over time. This study contributes to the evidence base of systemic risks during health crises and emergency response.
A1BG-AS1 promotes adriamycin resistance of breast cancer by recruiting IGF2BP2 to upregulate ABCB1 in an m6A-dependent manner
Authors: Wang Jian , Xu Jie , Zheng Jie
Published: November 2023

Adriamycin (ADR) resistance is an obstacle for chemotherapy of breast cancer (BC). ATP binding cassette subfamily B member 1 (ABCB1) expression is indicated to be closely related to the drug resistance of cancer cells. The current work intended to explore the molecular mechanisms to regulate ABCB1 in BC cells with ADR resistance. We found that long noncoding RNA (lncRNA) A1BG antisense RNA 1 (A1BG-AS1) is upregulated in ADR resistant BC cell lines (MCF-7/ADR, MDA-MB-231/ADR). A1BG-AS1 knockdown enhanced the ADR sensitivity by suppressing the viability, proliferation potential and migration ability, and facilitating cell apoptosis in BC. Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is known to be an m6A reader to modulate the stability of mRNA transcripts in an m6A-dependent manner, which was a shared RNA binding protein (RBP) for A1BG-AS1 and ABCB1. The interaction of IGF2BP2 with A1BG-AS1 or ABCB1 was explored and verified using RNA pulldown and RNA immunoprecipitation (RIP) assays. ABCB1 mRNA and protein expression was positively regulated by A1BG-AS1 and IGF2BP2 in BC cells. ABCB1 mRNA expression was stabilized by A1BG-AS1 via recruiting IGF2BP2 in an m6A-dependent manner. Moreover, rescue assays demonstrated that A1BG-AS1 enhanced BC ADR resistance by positively modulating ABCB1. Xenograft mouse models were used to explore whether A1BG-AS1 affected the ADR resistance in BC in vivo. The findings indicated that A1BG-AS1 silencing inhibited tumor growth and alleviated ADR resistance in vivo. In conclusion, A1BG-AS1 enhances the ADR resistance of BC by recruiting IGF2BP2 to upregulate ABCB1 in an m6A-dependent manner.
Influence of stimulus manipulation on conscious awareness of emotional facial expressions in the match-to-sample paradigm
Authors: Sato Wataru , Yoshikawa Sakiko
Published: November 2023

The conscious perception of emotional facial expressions plays an indispensable role in social interaction. However, previous psychological studies have reported inconsistent findings regarding whether conscious awareness is greater for emotional expressions than for neutral expressions. Furthermore, whether this phenomenon is attributable to emotional or visual factors remains unknown. To investigate these issues, we conducted five psychological experiments to test the conscious perception of emotional and neutral facial expressions using the match-to-sample paradigm. Facial stimuli were momentarily presented in the peripheral visual fields while participants read simultaneously presented letters in the central visual fields. The participants selected a perceived face from nine samples. The results of all experiments demonstrated that emotional expressions were more accurately identified than neutral expressions. Furthermore, Experiment 4 showed that angry expressions were identified more accurately than anti-angry expressions, which expressed neutral emotions with comparable physical changes to angry expressions. Experiment 5, testing the interaction between emotional expression and face direction, showed that angry expressions looking toward participants were more accurately identified than those looking away from participants, even though they were physically identical. These results suggest that the conscious awareness of emotional facial expressions is enhanced by their emotional significance.
Genetic diversity and historical demography of underutilised goat breeds in North-Western Europe
Authors: Manunza Arianna , Ramirez-Diaz Johanna , Cozzi Paolo , Lazzari Barbara , Tosser-Klopp Gwenola , Servin Bertrand , Johansson Anna M. , Grøva Lise , Berg Peer , Våge Dag Inge , Stella Alessandra
Published: November 2023

In the last decade, several studies aimed at dissecting the genetic architecture of local small ruminant breeds to discover which variations are involved in the process of adaptation to environmental conditions, a topic that has acquired priority due to climate change. Considering that traditional breeds are a reservoir of such important genetic variation, improving the current knowledge about their genetic diversity and origin is the first step forward in designing sound conservation guidelines. The genetic composition of North-Western European archetypical goat breeds is still poorly exploited. In this study we aimed to fill this gap investigating goat breeds across Ireland and Scandinavia, including also some other potential continental sources of introgression. The PCA and Admixture analyses suggest a well-defined cluster that includes Norwegian and Swedish breeds, while the crossbred Danish landrace is far apart, and there appears to be a close relationship between the Irish and Saanen goats. In addition, both graph representation of historical relationships among populations and f4-ratio statistics suggest a certain degree of gene flow between the Norse and Atlantic landraces. Furthermore, we identify signs of ancient admixture events of Scandinavian origin in the Irish and in the Icelandic goats. The time when these migrations, and consequently the introgression, of Scandinavian-like alleles occurred, can be traced back to the Viking colonisation of these two isles during the Viking Age (793-1066 CE). The demographic analysis indicates a complicated history of these traditional breeds with signatures of bottleneck, inbreeding and crossbreeding with the improved breeds. Despite these recent demographic changes and the historical genetic background shaped by centuries of human-mediated gene flow, most of them maintained their genetic identity, becoming an irreplaceable genetic resource as well as a cultural heritage.
Phylogenetic structure of body shape in a diverse inland ichthyofauna
Authors: Torgersen Kevin T. , Bouton Bradley J. , Hebert Alyx R. , Kleyla Noah J. , Plasencia Xavier , Rolfe Garrett L. , Tagliacollo Victor A. , Albert James S.
Published: November 2023

Body shape is a fundamental metric of animal diversity affecting critical behavioral and ecological dynamics and conservation status, yet previously available methods capture only a fraction of total body-shape variance. Here we use structure-from-motion (SFM) 3D photogrammetry to generate digital 3D models of adult fishes from the Lower Mississippi Basin, one of the most diverse temperate-zone freshwater faunas on Earth, and 3D geometric morphometrics to capture morphologically distinct shape variables, interpreting principal components as growth fields. The mean body shape in this fauna resembles plesiomorphic teleost fishes, and the major dimensions of body-shape disparity are similar to those of other fish faunas worldwide. Major patterns of body-shape disparity are structured by phylogeny, with nested clades occupying distinct portions of the morphospace, most of the morphospace occupied by multiple distinct clades, and one clade (Acanthomorpha) accounting for over half of the total body shape variance. In contrast to previous studies, variance in body depth (59.4%) structures overall body-shape disparity more than does length (31.1%), while width accounts for a non-trivial (9.5%) amount of the total body-shape disparity.
Therapeutic effects and potential mechanisms of endoscopic submucosal injection of mesenchymal stem cells on chronic atrophic gastritis
Authors: Xu Qianqian , Liu Mingyue , Meng Rui , Zhao Qi , Men Xiaoxiao , Lan Yadi , Xu Hongwei
Published: November 2023

Previous studies have demonstrated the rejuvenating and restorative actions of mesenchymal stem cells (MSCs) in multiple diseases, but their role in reversing chronic atrophic gastritis (CAG) is not well understood owing to their low efficiency in homing to the stomach. In this work, we investigated the therapeutic effect of umbilical cord-derived MSCs (UC-MSCs) on CAG by endoscopic submucosal injection and preliminarily explored possible mechanisms in vitro. MSCs and normal saline (NS) were injected into the submucosa of the stomach in randomly grouped CAG rabbits. Therapeutic effects on serum indices and histopathology of the gastric mucosa were analyzed in vivo at 30 and 60 days after MSCs injection. GES-1 cells were co-cultured with MSCs in vitro using a Transwell system and cell viability, proliferation, and migration ability were detected. Additionally, in view of the potential mechanisms, the relative protein expression levels of apoptosis, autophagy and inflammation in vitro were explored by Western Blotting. We found that submucosal injection of MSCs up-regulated serum indices (G-17, PGI and PGI/PGII) and alleviated histopathological damage to the gastric mucosa in CAG rabbits. Co-culture of GES-1 cells with MSCs improved cell viability, proliferation, and migration ability, while suppressing apoptosis. We also observed a reduction in the expression of apoptosis indicators, including Bax and cleaved caspase-3, in GES-1 cells after co-culture with MSCs in vitro. Our findings suggest that submucosal injection of MSCs is a promising approach for reversing CAG, and attenuating apoptosis plays a potential role in this process.
Response of a three-species cyclic ecosystem to a short-lived elevation of death rate
Authors: Chatterjee Sourin , De Rina , Hens Chittaranjan , Dana Syamal K. , Kapitaniak Tomasz , Bhattacharyya Sirshendu
Published: November 2023

A balanced ecosystem with coexisting constituent species is often perturbed by different natural events that persist only for a finite duration of time. What becomes important is whether, in the aftermath, the ecosystem recovers its balance or not. Here we study the fate of an ecosystem by monitoring the dynamics of a particular species that encounters a sudden increase in death rate. For exploration of the fate of the species, we use Monte-Carlo simulation on a three-species cyclic rock-paper-scissor model. The density of the affected (by perturbation) species is found to drop exponentially immediately after the pulse is applied. In spite of showing this exponential decay as a short-time behavior, there exists a region in parameter space where this species surprisingly remains as a single survivor, wiping out the other two which had not been directly affected by the perturbation. Numerical simulations using stochastic differential equations of the species give consistency to our results.
Evaluation of haematological, genotoxic, cytotoxic and ATR-FTIR alterations in blood cells of fish Channa punctatus after acute exposure of aniline
Authors: Sharma Geetika , Chadha Pooja
Published: November 2023

Aniline (C6H5NH2) an important intermediate in the organic and fine chemical industry, is ubiquitously used worldwide. It is one of the important building block for manufacturing of 4,4-methylene diphenyl diisocyanate (MDI), accelerators in rubber processing, dyes, tattoo inks, photographic chemicals, antioxidants, corrosion inhibitors, pharmaceuticals and antiseptics. The current study evaluated 96 h LC50 of aniline and based on this, two sublethal concentrations (4.19 mg/l and 8.39 mg/l) were selected for acute exposure studies in freshwater food fish Channa punctatus. Erythrocytes of fish are nucleated hence they play an important role in physiology, immune system, protein signalling and haemostatic condition along with respiration. Blood samples were collected after 24, 48, 72, and 96 h of exposure to study haematological, cytotoxic and genotoxic effects of sublethal concentrations of aniline in C. punctatus. Symbolic elevation in time and dose dependent DNA damage was observed by comet assay as well as micronuclei assay revealing maximum damage after 96 h of exposure. After aniline exposure, scanning electron microscopy and ATR-FTIR studies showed anomalies in structure and alterations in biomolecules of RBCs of aniline exposed group as compared to control group respectively. Semi prep HPLC studies revealed bioaccumulation potential of aniline in higher concentration exposed group.
Monitoring the process mean under the Bayesian approach with application to hard bake process
Authors: Khan Imad , Noor-ul-Amin Muhammad , Khan Dost Muhammad , Ismail Emad A. A. , Yasmeen Uzma , Rahimi Javed
Published: November 2023

This study introduces the Bayesian adaptive exponentially weighted moving average (AEWMA) control chart within the framework of measurement error, examining two separate loss functions: the squared error loss function and the linex loss function. We conduct an analysis of the posterior and posterior predictive distributions utilizing a conjugate prior. In the presence of measurement error (ME), we employ a linear covariate model to assess the control chart's effectiveness. Additionally, we explore the impacts of measurement error by investigating multiple measurements and a method involving linearly increasing variance. We conduct a Monte Carlo simulation study to assess the control chart's performance under ME, examining its run length profile. Subsequently, we offer a specific numerical instance related to the hard-bake process in semiconductor manufacturing, serving to verify the functionality and practical application of the suggested Bayesian AEWMA control chart when confronted with ME.
Increased expression of individual genes in whole blood is associated with late-stage lung cancer at and close to diagnosis
Authors: Urbarova Ilona , Skogholt Anne Heidi , Sun Yi-Qian , Mai Xiao-Mei , Grønberg Bjørn Henning , Sandanger Torkjel Manning , Sætrom Pål , Nøst Therese Haugdahl
Published: November 2023

Lung cancer (LC) mortality rates are still increasing globally. As survival is linked to stage, there is a need to identify markers for earlier LC diagnosis and individualized treatment. The whole blood transcriptome of LC patients represents a source of potential LC biomarkers. We compared expression of > 60,000 genes in whole blood specimens taken from LC cases at diagnosis (n = 128) and controls (n = 62) using genome-wide RNA sequencing, and identified 14 candidate genes associated with LC. High expression of ANXA3, ARG1 and HP was strongly associated with lower survival in late-stage LC cases (hazard ratios (HRs) = 2.81, 2.16 and 2.54, respectively). We validated these markers in two independent population-based studies with pre-diagnostic whole blood specimens taken up to eight years prior to LC diagnosis (n = 163 cases, 184 matched controls). ANXA3 and ARG1 expression was strongly associated with LC in these specimens, especially with late-stage LC within two years of diagnosis (odds ratios (ORs) = 3.47 and 5.00, respectively). Additionally, blood CD4 T cells, NK cells and neutrophils were associated with LC at diagnosis and improved LC discriminative ability beyond candidate genes. Our results indicate that in whole blood, increased expression levels of ANXA3, ARG1 and HP are diagnostic and prognostic markers of late-stage LC.
Retroperitoneal totally endoscopic prosthetic repair of lumbar hernia
Authors: Li Haoran , Cheng Zhengwu , Yan Wenwu , Hu Xunzi , Wang Junfeng
Published: November 2023

Lumbar hernia is a rare lateral abdominal wall hernia. Various surgical repair strategies have been recorded, but there is currently no unified standard. A Chinese surgeon recently revealed a novel technique for treating primary lumbar hernia called retroperitoneal totally endoscopic prosthetic repair (R-TEP). We have made a further exploration of this method and successfully used it in the treatment of secondary lumbar hernia. We successfully performed R-TEP on three patients with lumbar hernias. All patients were female with an average age of 64 years (51–71 years). Two patients each had a primary upper lumbar hernia, while one patient had a secondary lumbar hernia. With a mean operative time of 77 min (60–105 min), all operations were performed successfully. The average visual analogue scale (VAS) was 1.3 points (1–2 points) on the second day following surgery. The mean postoperative hospital stay was 2.3 days (2–3 days). No postoperative complications occurred. During a mean follow-up period of 19 months (10–24 months), there was no recurrence of the hernia, chronic pain or mesh infection. Therefore, R-TEP is safe and effective for both primary and secondary lumbar hernia. Anti-adhesive coated meshes are not required, making this a cost-effective procedure that is worthy of recommendation.
Effect of polyethylene terephthalate particles on filamentous bacteria involved in activated sludge bulking and improvement in sludge settleability
Authors: Wei Su , Huang Ziyang , Ni Yongjiong , Pan Zengrui , Feng Hongbo , Cheng Xiaoyu , Huang Zuchao , Liao Hanglei , Li Jun
Published: November 2023

Excessive proliferation of filamentous bacteria within activated sludge leads to sludge structural instability and diminished settling properties, which is a prevalent issue in tannery wastewater treatment. Based on available information, there is a lack of research on the impact of particle addition on filamentous bacteria in activated sludge, specifically with respect to a reduction in sludge bulking. Therefore, polyethylene terephthalate (PET) was selected as the test material to elucidate the effect of particles on sludge bulking. The results illustrate that particles measuring 0.1 mm in diameter have a profound influence on both the quantity and morphological characteristics of filamentous bacteria in activated sludge. In an anaerobic-aoxic-oxic (AAO) reactor, the use of 4000 particles/L led to a significant decrease in the sludge volume index (SVI), reducing it from 358 mg/L to 198 mg/L. The results offer significant insights for resolving sludge bulking problems in tannery wastewaters. Moreover, the results are significant as a reference point for future investigations on the efficacy of incorporating diverse particulate materials to ameliorate issues associated with activated sludge bulking.
Investigation of microbial fuel cell performance based on the nickel thin film modified electrodes
Authors: Mahmoodzadeh Fatemeh , Navidjouy Nahid , Alizadeh Saber , Rahimnejad Mostafa
Published: November 2023

Microbial fuel cells (MFCs) are a self-sustaining and environmentally friendly system for the simultaneous was tewater treatment and bioelectricity generation. The type and material of the electrode are critical factors that can influence the efficiency of this treatment process. In this study, graphite plates and carbon felt were modified through the electrodeposition of nickel followed by the formation of a biofilm, resulting in conductive bio-anode thin film electrodes with enhanced power generation capacity. The structural and morphological properties of the electrode surfaces were characterized using X-ray diffraction, energy-dispersive X-ray spectroscopy, elemental mapping, and field-emission scanning electron microscopy techniques. Maximum voltage, current density, and power generation were investigated using a dual-chamber MFC equipped with a Nafion 117 membrane and bio-nickel-doped carbon felt (bio-Ni@CF) and bio-nickel-doped graphite plate (bio-Ni@GP) electrodes under constant temperature conditions. The polarization and power curves obtained using different anode electrodes revealed that the maximum voltage, power and current density achieved with the bio-Ni@CF electrode were 468.0 mV, 130.72 mW/m2 and 760.0 mA/m2 respectively. Moreover, the modified electrodes demonstrated appropriate stability and resistance during successful runs. These results suggest that nickel-doped carbon-based electrodes can serve as suitable and stable supported catalysts and conductors for improving efficiency and increasing power generation in MFCs.
Network representations of attractors for change point detection
Authors: Tan Eugene , Algar Shannon D. , Corrêa Débora , Stemler Thomas , Small Michael
Published: November 2023

A common approach to monitoring the status of physical and biological systems is through the regular measurement of various system parameters. Changes in a system’s underlying dynamics manifest as changes in the behaviour of the observed time series. For example, the transition from healthy cardiac activity to ventricular fibrillation results in erratic dynamics in measured electrocardiogram (ECG) signals. Identifying these transitions—change point detection—can be valuable in preparing responses to mitigate the effects of undesirable system changes. Here, we present a data-driven method of detecting change points using a phase space approach. Delay embedded trajectories are used to construct an ‘attractor network’, a discrete Markov-chain representation of the system’s attractor. Once constructed, the attractor network is used to assess the level of surprise of future observations where unusual movements in phase space are assigned high surprise scores. Persistent high surprise scores indicate deviations from the attractor and are used to infer change points. Using our approach, we find that the attractor network is effective in automatically detecting the onset of ventricular fibrillation (VF) from observed ECG data. We also test the flexibility of our method on artificial data sets and demonstrate its ability to distinguish between normal and surrogate time series.
Cities and regions tackle climate change mitigation but often focus on less effective solutions
Authors: Burley Farr Katherine , Song Kaihui , Yeo Zhi Yi , Johnson Evan , Hsu Angel
Published: November 2023

Although the potential for cities and regions to contribute to global mitigation efforts is widely acknowledged, there is little evidence on the effectiveness of subnational mitigation strategies. Here we address this gap through a systematic review of 234 quantitative mitigation case studies. We use a meta-analytical approach to estimate expected greenhouse gas emissions reductions from 12 categories of mitigation strategies. We find that strategies related to land use and development, circular economy, and waste management are most effective and reliable for reducing emissions. The results demonstrate that cities and regions are taking widespread action to reduce emissions. However, we find misalignment between the strategies that policymakers and researchers focus on, compared to those with the highest expected impacts. The results inform climate action planning at the city and regional level and the evaluation of subnational climate targets.
Two-dimensional ternary pentagonal BCX (X = P, As, and Sb): promising photocatalyst semiconductors for water splitting with strong piezoelectricity
Authors: Liu Luqi , Kang Xuxin , Gao Shan , Duan Xiangmei
Published: November 2023

The proposed penta-BCX (X = P, As, and Sb) has low Young’s modulus and strong piezoelectricity. Strain can modulate their band edge positions and light absorption, thus enhancing their photocatalytic performance in the visible light and UV regions.