bluuebunny/crossref_metadata_2025 huggingface dataset:
Survivors of a Kamikaze Attack: PTSD and Perceived Adjustment to Civilian Life
Authors: Chara Paul J. , Chara Kathleen A.
Published: December 2010

30 survivors of the U.S.S. Emmons ( M age = 81.3 yr., SD = 2.3), which was sunk by kamikaze attacks during World War II, were given an adapted form of the Posttraumatic Stress Disorder Checklist-Civilian and a set of questions regarding the effects of their World War II experiences on seven clusters of 42 interpersonal and personal characteristics (Adjustment Characteristics). Participants were categorized into two groups, those who served on the Emmons at any given time during World War II (Any Service), and those who were serving on the Emmons when it was sunk during the battle for Okinawa (Okinawa). Analyses using Mann-Whitney U tests indicated that the Okinawa group reported higher checklist scores, but the scores were unrelated to 41 of the 42 Adjustment Characteristics. Also, Spearman rank-order correlations were statistically insignificant for all comparisons between checklist scores and Adjustment Characteristic clusters, and the total number of positive responses to the 42 characteristics. In contrast, Mann-Whitney U and chi square analyses comparing the Any Service and Okinawa groups indicated statistically significant differences for 14 Adjustment Characteristics, 4 clusters of those characteristics, and the total number of positive responses: The Okinawa group reported poorer outcomes in each comparison. Many of these differences were among Adjustment Characteristics evocative of the attributes of Lee's Agape love style. The findings suggest that for the survivors of the Emmons, the presence or absence of a traumatic event was a better predictor of adjustment to civilian life than the reported severity of PTSD symptoms.
Attitudes toward Empathy in Domestic Dogs and Cats
Authors: Vitulli William F.
Published: December 2010

This exploratory study included 524 undergraduate students (360 women, Mage = 21.1 yr., SD = 6.1; and 164 men, Mage = 20.0 yr., SD = 3.6) enrolled in introductory psychology sections who completed the Empathy in Pet Dogs and Cats Scale to assess their attitudes regarding how much domestic dogs and cats show vicarious experiencing of the thoughts, feelings, or attitudes of their owners. A principal components analysis followed by varimax rotation extracted five factors accounting for 60.85% of the total variance as follows: (1) Pets' empathy for owner, (2) Cats' empathy for owner, (3) Dogs' empathy for owner, (4) Souls in humans and pets, (5) Owners' empathy for pets. Women gave significantly higher ratings than men regarding dogs' and cats' knowing when their owners are happy, sad, or angry. Women also gave significantly higher ratings than men regarding dogs' and cats' feeling love and compassion for their owners. Specific instances of empathy in pets underscore the need for contemporary research to be inclusive of operational definitions of measures of empathy.
Can Money Buy Happiness?
Authors: Spinella Marcello , Lester David
Published: December 2010

Income was negatively associated with the mood states of confusion and tense in a sample of 406 community residents, but controls for age, sex, and education eliminated these associations.
Visiting Santa: A Further Look
Authors: Trinkaus John
Published: December 2010

An informal enquiry of the facial expressions of children as they queued up to meet Santa Claus during the 2003 Yuletide season showed that about 8 out of 10 children appeared to be indifferent to seeing Santa. This replication of the study in 2004 resulted in an approximately similar finding. A repeat of these enquiries, in 2005, suggested that the indifference rate appeared to lessen (being somewhat more cheerful) the closer was Christmas.
Score Reliability for Tests Constructed for African-American Populations
Authors: Charter Richard A.
Published: December 2010

Test score reliabilities and sample sizes (N) used to establish the reliabilities are described for a variety of tests constructed for African-American populations. The sample size was 341. The average internal consistency reliability was .74 ( SD = .16) with a median value of .77. The median N was 131. The mean internal consistency reliability and median N for tests intended for assessment of individuals were only .72 and 96, respectively.
Analysis of airborne metal containing particles with EDX/EDS detectors in electron microscopes
Authors: Sielicki Przemysław , Janik Helena , Guzman Agnieszka , Reynolds Alan , Namieśnik Jacek
Published: December 2010

In years 2006–2010 particulate matter analysis was undertaken for dust samples collected from Gdansk and London area in order to compare their morphology and composition. Part of those studies was devoted to analysis of particulate matter (PM) bearing metals. Characterization of the morphology and size of the particles collected onto the filters was performed using a scanning electron microscope (SEM) and transmission electron microscope (TEM). Both electron microscopes were equipped with energy dispersive X-ray spectrometers to identify the elemental composition of the particles. On analysis of the X-ray spectra acquired by both TEM and SEM, the particles were divided into 10 groups as follows: Al-rich, Ba-rich, C-rich, Ca-rich, Cl-rich, Fe-rich, Mg-rich, Na-rich, S-rich, Si-rich. Speciation of the particles based on the major element and accompanying minor elements yielded 34 particle types. However, some pairs of elements repeat, for instance: Na-Cl and Cl-Na, Al-S and S-Al, Si-Al and Al-Si, S-Ca and Ca-S. These are undoubtedly the same types of particles; variation in peak heights of the major and minor elements is normal in a mixed particle population.
Karhunen-Loève expansions of α-Wiener bridges
Authors: Barczy Mátyás , Iglói Endre
Published: December 2010

We study Karhunen-Loève expansions of the process(X
t(α))t∈[0,T) given by the stochastic differential equation $$
dX_t^{(\alpha )} = - \frac{\alpha }
{{T - t}}X_t^{(\alpha )} dt + dB_t ,t \in [0,T)
$$, with the initial condition X
0(α) = 0, where α > 0, T ∈ (0, ∞), and (B
t)t≥0 is a standard Wiener process. This process is called an α-Wiener bridge or a scaled Brownian bridge, and in the special case of α = 1 the usual Wiener bridge. We present weighted and unweighted Karhunen-Loève expansions of X
(α). As applications, we calculate the Laplace transform and the distribution function of the L
2[0, T]-norm square of X
(α) studying also its asymptotic behavior (large and small deviation).
Blow-up of the solution for higher-order Kirchhoff-type equations with nonlinear dissipation
Authors: Gao Qingyong , Li Fushan , Wang Yanguo
Published: December 2010

In this paper, we consider the nonlinear Kirchhoff-type equation $$
u_{tt} + M(\left\| {D^m u(t)} \right\|_2^2 )( - \Delta )^m u + \left| {u_t } \right|^{q - 2} u_t = \left| {u_t } \right|^{p - 2} u
$$ with initial conditions and homogeneous boundary conditions. Under suitable conditions on the initial datum, we prove that the solution blows up in finite time.
The use of the fluidized bed boiler for the disposal of the multi-material packaging waste
Authors: Zabagło Jadwiga , Baron Jerzy , Olek Małgorzata , Kandefer Stanisław , Żukowski Witold
Published: December 2010

The use of the fluidized bed boiler for the disposal of the multi-material packaging waste

The paper presents the results of the disposal of packaging waste from two companies: Tetra Pak and Combibloc, carried out in a fluidized bed boiler of rated thermal power 0.5 MW. The material introduced into the fluidized bed boiler underwent thermal and mechanical degradation in a sand bed of the temperature between 750 and 850°C. The process proceeds auto-thermally, without the need of additional fuel. The appropriately chosen fluidization parameters caused the separation of the solid products of combustion from the deposit material. Presence of aluminum, part of it in an un-oxidized form, was confirmed in separated dust. The gaseous products of combustion contained the traces of oxides of nitrogen and sulfur, mainly originating from the remnants of food products contained in the packaging. However, the concentration of these oxides met the requirements of emission standards.
Kinetics studies and mechanism evolution of the epoxidation of styrene over nanoporous Au doped TS-1
Authors: Saxena Samidha , Dwivedi Reena , Bhadauria Sheenu , Chumbhale V. , Prasad Rajendra
Published: December 2010

Kinetics studies and mechanism evolution of the epoxidation of styrene over nanoporous Au doped TS-1

A kinetic investigation of the slurry phase epoxidation of styrene with hydrogen peroxide has been carried out, for the first time, over nanoporous Au doped TS-1 catalyst, in a batch reactor, in the temperature range of 313-333 K. It was found that product selectivity and the rate of reaction are greatly influenced by concentrations of styrene and hydrogen peroxide. Kinetics studies reveal that the mechanism of the reaction is of the "Redox" type. The rate equation, r = k1 k2 PO PH / (k1 PO + k2 PH) deduced, assuming a steady state involving two stage oxidation-reduction process, represent the data most satisfactorily for the conversion of styrene to styrene oxide. A tentative mechanism of the process has also been suggested.
Australian National Trends in Stimulant Dispensing: 2002–2009
Authors: Hollingworth Samantha A. , Nissen Lisa M. , Stathis Stephen S. , Siskind Dan J. , Varghese John M. N. , Scott James G.
Published: December 2010

Objective: Internationally there has been an increase in the prescriptions of stimulant medication. The aim of this study was to examine longitudinal national trends of stimulant dispensing in Australia between 2002 and 2009.

Method: Government databases were retrospectively reviewed for all dispensed stimulant prescriptions between 2002 and 2009. Prescriptions were converted to defined daily dose (DDD)/1000 population/day using census data. Utilization of dexamphetamine and methylphenidate were analysed by source (subsidized or non-subsidized), prescriber (general practitioner, psychiatrist or other specialists), gender and age of patient.

Results: Between 2002 and 2009, dispensing of stimulants in Australia increased 87% from 2.93 to 5.47 DDD/1000 population/day. Dexamphetamine remained the most commonly dispensed stimulant, with rates of dispensing falling 13% from 2.02 to 1.75 DDD/1000 population/day. Dispensed prescriptions of methylphenidate increased 300% from 0.45 in 2002 to 1.81 DDD/1000 population/day in 2009, attributable to the availability of long-acting preparations. Dispensing of stimulants to males was four-fold greater than to females. There was substantial dispensing of dexamphetamine to those older than 25 years.

Conclusions: Stimulant dispensing in Australia increased between 2002 and 2009 as a result of increased dispensing of long-acting preparations of methylphenidate. Further research is required to determine if the increase in stimulant dispensing in Australia is clinically appropriate.
Screening Technologies for Target Identification in Pancreatic Cancer
Authors: Michl Patrick , Ripka Stefanie , Gress Thomas , Buchholz Malte
Published: December 2010

Pancreatic cancer exhibits an extraordinarily high level of resistance to almost any kind of systemic therapy evaluated in clinical trials so far. Therefore, the identification of novel therapeutic targets is urgently required. High-throughput screens have emerged as an important tool to identify putative targets for diagnosis and therapy in an unbiased manner. More than a decade ago, microarray technology was introduced to identify differentially expressed genes in pancreatic cancer as compared to normal pancreas, chronic pancreatitis and other cancer types located in close proximity to the pancreas. In addition, proteomic screens have facilitated the identification of differentially secreted proteins in body fluids of pancreatic cancer patients, serving as possible biomarkers. Recently, RNA interference-based loss-of-function screens have been used to identify functionally relevant genes, whose knock-down has impact on pancreatic cancer cell viability, thereby representing potential new targets for therapeutic intervention. This review summarizes recent results of transcriptional, proteomic and functional screens in pancreatic cancer and discusses potentials and limitations of the respective technologies as well as their impact on future therapeutic developments.
A Unique Perspective on Data Coding and Decoding
Authors: Wang Wen-Yan
Published: December 2010

The concept of a loss-less data compression coding method is proposed, and a detailed description of each of its steps follows. Using the Calgary Corpus and Wikipedia data as the experimental samples and compared with existing algorithms, like PAQ or PPMstr, the new coding method could not only compress the source data, but also further re-compress the data produced by the other compression algorithms. The final files are smaller, and by comparison with the original compression ratio, at least 1% redundancy could be eliminated. The new method is simple and easy to realize. Its theoretical foundation is currently under study. The corresponding Matlab source code is provided in the Appendix.
Spatial Variation and Distribution of Urban Energy Consumptions from Cities in China
Authors: Zhang Lixiao , Yang Zhifeng , Liang Jing , Cai Yanpeng
Published: December 2010

With support of GIS tools and Theil index, the spatial variance of urban energy consumption in China was discussed in this paper through the parallel comparison and quantitative analysis of the 30 provincial capital cities of mainland China in 2005, in terms of scale, efficiency and structure. The indicators associated with urban energy consumption show large spatial variance across regions, possibly due to diversities of geographic features, economic development levels and local energy source availability in China. In absolute terms, cities with the highest total energy consumption are mostly distributed in economic-developed regions as Beijing-Tianjin-Tangshan Area, Yangtze River Delta and Pearl River Delta of China, however, the per capita urban energy use is significantly higher in the Mid-and-Western regions. With regard to the energy mix, coal still plays the dominant role and cities in Mid-and-Western regions rely more on coal. In contrast, high quality energy carrier as electricity and oils are more used in southeast coastal zone and northern developed areas. The energy intensive cities are mainly located in the northwest, while the cities with higher efficiency are in southeast areas. The large spatial variance of urban energy consumption was also verified by the Theil indices. Considering the Chinese economy-zones of East, Middle and West, the within-group inequalities are the main factor contributing to overall difference, e.g., the Theil index for per capita energy consumption of within-group is 0.18, much higher than that of between group (0.07), and the same applies to other indicators. In light of the spatial variance of urban energy consumptions in China, therefore, regionalized and type-based management of urban energy systems is badly needed to effectively address the ongoing energy strategies and targets.
Landscape Transformation in Tropical Latin America: Assessing Trends and Policy Implications for REDD+
Authors: Pacheco Pablo , Aguilar-Støen Mariel , Börner Jan , Etter Andres , Putzel Louis , Diaz Maria del Carmen Vera
Published: December 2010

Important transformations are underway in tropical landscapes in Latin America with implications for economic development and climate change. Landscape transformation is driven not only by national policies and markets, but also by global market dynamics associated with an increased role for transnational traders and investors. National and global trends affect a disparate number of social, political and economic interactions taking place at the local level, which ultimately shapes land-use and socio-economic change. This paper reviews five different trajectories of landscape change in tropical Latin America, and discusses their implications for development and conservation: (1) Market-driven growth of agribusiness; (2) expansion and modernization of traditional cattle ranching; (3) slow growth of peasant agriculture; (4) logging in production forest frontiers; and (5) resurgence of agro-extractive economies. Contrasting trade-offs between economic development and forest conservation emerge across these landscapes, calling for nuanced policy responses to manage them in the context of climate change. This discussion sets the background to assess how reduction of emissions from deforestation and forest degradation and enhancing carbon stocks (REDD+) aims should be better aligned with current landscape trajectories and associated actors to better address climate-change mitigation in forest landscapes with effective and equitable outcomes.
Flexible and Asymmetric Ligand in Constructing Coordinated Complexes: Synthesis, Crystal Structures and Fluorescent Characterization
Authors: Chen Peng , Lin Jianhua
Published: December 2010

Flexible and asymmetric ligand L [L = 1-((pyridin-3-yl)methyl)-1H-benzotriazole], is used as a basic backbone to construct complicated metal-organic frameworks. Two new polymers, namely, [Ag2(L)2(NO3)2]n (1) and [Ag(L)(ClO4)]n (2), were synthesized and characterized by X-ray structure analysis and fluorescent spectroscopy. The complex 1 gives an “S” type double helical conformation, whereas complex 2 exhibits a 1D zigzag configuration. Different anions affect the silver coordination geometry and crystal packing topology.
The Chitinolytic Activities of Streptomyces sp. TH-11
Authors: Hoang Kim-Chi , Lai Tzu-Hsuan , Lin Chung-Sheng , Chen Ying-Tsong , Liau Chun-Yi
Published: December 2010

Chitin is an abundant biopolymer composed of units of N-acetyl-D-glucosamine linked by b-1,4 glycosidic bonds. Chitin is the main component of the shells of mollusks, the cell wall of fungi and yeast and of the exoskeleton of crustaceans and insects. The degradation of chitin is catalyzed by chitinases that occur in a wide range of organisms. Among them, the chitinases from microorganisms are extremely important for the degradation and recycling of the carbon and nitrogen trapped in the large amount of insoluble chitin in nature. Streptomyces sp. TH-11 was isolated from the sediment of the Tou-Chien River, Taiwan. The chitinolytic enzyme activities were detected using a rapid in-gel detection method from the cell-free preparation of the culture medium of TH-11. The chitinolytic enzyme activity during prolonged liquid culturing was also analyzed by direct measurement of the chitin consumption. Decomposition of the exoskeleton of shrimps was demonstrated using electron microscopy and atomic force microscopy.
Active Microwave Metamaterials Incorporating Ideal Gain Devices
Authors: Si Li-Ming , Jiang Tao , Chang Kihun , Chen Te-Chuan , Lv Xin , Ran Lixin , Xin Hao
Published: December 2010

Incorporation of active devices/media such as transistors for microwave and gain media for optics may be very attractive for enabling desired low loss and broadband metamaterials. Such metamaterials can even have gain which may very well lead to new and exciting physical phenomena. We investigate microwave composite right/left-handed transmission lines (CRLH-TL) incorporating ideal gain devices such as constant negative resistance. With realistic lumped element values, we have shown that the negative phase constant of this kind of transmission lines is maintained (i.e., left-handedness kept) while gain can be obtained (negative attenuation constant of transmission line) simultaneously. Possible implementation and challenging issues of the proposed active CRLH-TL are also discussed.
Low Water Activity Induces the Production of Bioactive Metabolites in Halophilic and Halotolerant Fungi
Authors: Sepcic Kristina , Zalar Polona , Gunde-Cimerman Nina
Published: December 2010

The aim of the present study was to investigate indigenous fungal communities isolated from extreme environments (hypersaline waters of solar salterns and subglacial ice), for the production of metabolic compounds with selected biological activities: hemolysis, antibacterial, and acetylcholinesterase inhibition. In their natural habitats, the selected fungi are exposed to environmental extremes, and therefore the production of bioactive metabolites was tested under both standard growth conditions for mesophilic microorganisms, and at high NaCl and sugar concentrations and low growth temperatures. The results indicate that selected halotolerant and halophilic species synthesize specific bioactive metabolites under conditions that represent stress for non-adapted species. Furthermore, adaptation at the level of the chemical nature of the solute lowering the water activity of the medium was observed. Increased salt concentrations resulted in higher hemolytic activity, particularly within species dominating the salterns. The appearance of antibacterial potential under stress conditions was seen in the similar pattern of fungal species as for hemolysis. The active extracts exclusively affected the growth of the Gram-positive bacterium tested, Bacillus subtilis. None of the extracts tested showed inhibition of acetylcholinesterase activity.
Secondary Metabolites from a Marine-Derived Endophytic Fungus Penicillium chrysogenum QEN-24S
Authors: Gao Shu-Shan , Li Xiao-Ming , Du Feng-Yu , Li Chun-Shun , Proksch Peter , Wang Bin-Gui
Published: December 2010

Penicillium chrysogenum QEN-24S, an endophytic fungus isolated from an unidentified marine red algal species of the genus Laurencia, displayed inhibitory activity against the growth of pathogen Alternaria brassicae in dual culture test. Chemical investigation of this fungal strain resulted in the isolation of four new (1–3 and 5) and one known (4) secondary metabolites. Their structures were identified as two polyketide derivatives penicitides A and B (1 and 2), two glycerol derivatives 2-(2,4-dihydroxy-6-methylbenzoyl)-glycerol (3) and 1-(2,4-dihydroxy-6-methylbenzoyl)-glycerol (4), and one monoterpene derivative penicimonoterpene (5). Penicitides A and B (1 and 2) feature a unique 10-hydroxy- or 7,10-dihydroxy-5,7-dimethylundecyl moiety substituting at C-5 of the α-tetrahydropyrone ring, which is not reported previously among natural products. Compound 5 displayed potent activity against the pathogen A. brassicae, while compound 1 exhibited moderate cytotoxic activity against the human hepatocellular liver carcinoma cell line.
Synthesis of Carbon Nanotube (CNT) Composite Membranes
Authors: Altalhi Tariq , Ginic-Markovic Milena , Han Ninghui , Clarke Stephen , Losic Dusan
Published: December 2010

Carbon nanotubes are attractive approach for designing of new membranes for advanced molecular separation because of their unique transport properties and ability to mimic biological protein channels. In this work the synthetic approach for fabrication of carbon nanotubes (CNTs) composite membranes is presented. The method is based on growth of multi walled carbon nanotubes (MWCNT) using chemical vapour deposition (CVD) on the template of nanoporous alumina (PA) membranes. The influence of experimental conditions including carbon precursor, temperature, deposition time, and PA template on CNT growth process and quality of fabricated membranes was investigated. The synthesis of CNT/PA composites with controllable nanotube dimensions such as diameters (30–150 nm), and thickness (5–100 µm), was demonstrated. The chemical composition and morphological characteristics of fabricated CNT/PA composite membranes were investigated by various characterisation techniques including scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDXS), high resolution transmission electron microscopy (HRTEM) and x-ray diffraction (XRD). Transport properties of prepared membranes were explored by diffusion of dye (Rose Bengal) used as model of hydrophilic transport molecule.
Novel Synthesis and Antitumor Evaluation of Polyfunctionally Substituted Heterocyclic Compounds Derived from 2-Cyano-N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-acetamide
Authors: Shams Hoda Z. , Mohareb Rafat M. , Helal Maher H. , Mahmoud Amira E.
Published: December 2010

The reaction of 2-amino-3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophene with ethyl cyanoacetate gave 2-cyano-N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-acetamide. The latter was used to synthesize different heterocyclic derivatives comprising thiophene, thiazole, pyrazole, pyridine, pyrimidine, and coumarin rings. The mechanistic and synthetic pathways depended on regioselective attack and/or cyclization by the cyanoacetamido moiety in the key precursor on various chemical reagents. The competition of the reaction pathways including dipolar cyclization, dinucleophilic-bielectrophilic attack, β-attack, Gewald-type attack, and condensation reactions led to the diversity of the synthesized products. The antitumor activities of the synthesized products were studied and evaluated. Most of the compounds revealed high inhibitory effects when screened in vitro for their antiproliferative activity. Three human cancer cell lines, namely, breast adenocarcinoma (MCF-7), non-small cell lung cancer (NCI-H460) and CNS cancer (SF-268) were used in the screening tests. The simplicity of the synthetic procedures which mainly involved one-pot reactions under mild reaction conditions, the convenience of yield production and the diversity of the reactive sites in the produced systems play a valuable role for further heterocyclic transformations and further biological investigations.
The Relationship between Phenolics and Flavonoids Production with Total Non Structural Carbohydrate and Photosynthetic Rate in Labisia pumila Benth. under High CO2 and Nitrogen Fertilization
Authors: Ibrahim Mohd Hafiz , Jaafar Hawa Z.E. , Rahmat Asmah , Rahman Zaharah Abdul
Published: December 2010

A factorial split plot 4 × 3 experiment was designed to examine and characterize the relationship among production of secondary metabolites (total phenolics, TP; total flavonoids, TF), carbohydrate content and photosynthesis of three varieties of the Malaysian medicinal herb Labisia pumila Benth. namely the varieties alata, pumila and lanceolata under CO2 enrichment (1,200 µmol mol-1) combined with four levels of nitrogen fertilization (0, 90, 180 and 270 kg N ha-1). No varietal differences were observed, however, as the levels of nitrogen increased from 0 to 270 kg N ha-1, the production of TP and TF decreased in the order leaves>roots>stems. The production of TP and TF was related to increased total non structural carbohydrate (TNC), where the increase in starch content was larger than that in sugar concentration. Nevertheless, the regression analysis exhibited a higher influence of soluble sugar concentration (r2 = 0.88) than starch on TP and TF biosynthesis. Photosynthesis, on the other hand, displayed a significant negative relationship with TP and TF production (r2 = -0.87). A decrease in photosynthetic rate with increasing secondary metabolites might be due to an increase in the shikimic acid pathway that results in enhanced production of TP and TF. Chlorophyll content exhibited very significant negative relationships with total soluble sugar, starch and total non structural carbohydrate.
Anti-PEG IgM Response against PEGylated Liposomes in Mice and Rats
Authors: Ichihara Masako , Shimizu Taro , Imoto Ami , Hashiguchi Yuki , Uehara Yumi , Ishida Tatsuhiro , Kiwada Hiroshi
Published: December 2010

We have reported that PEGylated liposomes lose their long-circulating properties when they are administered repeatedly at certain intervals to the same animal. This unexpected phenomenon is referred to as the accelerated blood clearance (ABC) phenomenon. We recently showed that the ABC phenomenon is triggered via the abundant secretion of anti-PEG IgM in response to the first dose of PEGylated liposomes. However, the details of the underlying mechanism for the induction of anti-PEG IgM production are yet to be elucidated. The present study demonstrated that the spleen is a major organ involved in the secretion of anti-PEG IgM in mice and rats. Anti-PEG IgM production was detected in nude, T-cell deficient mice, but not in SCID mice with B- and T-cell deficiencies. These observations indicate that splenic B-cells secret anti-PEG IgM without help from T-cells. Sequential injections of PEGylated liposomes into the same mice did not promote isotype switching from IgM to IgG. Accordingly, PEGylated liposomes may function as a type-2, T-cell-independent antigen (TI-2 antigen) during anti-PEG IgM production. Although the underlying mechanism that causes an anti-PEG IgM response against PEGylated liposomes is not yet clear, our findings give implications in revealing the anti-PEG IgM response against PEGylated liposome.
Mimicking the Nanostructure of Bone: Comparison of Polymeric Process-Directing Agents
Authors: Thula Taili T. , Svedlund Felicia , Rodriguez Douglas E. , Podschun Jacob , Pendi Laura , Gower Laurie B.
Published: December 2010

The nanostructure of bone has been replicated using a polymer-induced liquid-precursor (PILP) mineralization process. This polymer-mediated crystallization process yields intrafibrillar mineralization of collagen with uniaxially-oriented hydroxyapatite crystals. The process-directing agent, an anionic polymer which we propose mimics the acidic non-collagenous proteins associated with bone formation, sequesters calcium and phosphate ions to form amorphous precursor droplets that can infiltrate the interstices of collagen fibrils. In search of a polymeric agent that produces the highest mineral content in the shortest time, we have studied the influence of various acidic polymers on the in vitro mineralization of collagen scaffolds via the PILP process. Among the polymers investigated were poly-L-aspartic acid (PASP), poly-L-glutamic acid (PGLU), polyvinylphosphonic acid (PVPA), and polyacrylic acid (PAA). Our data indicate that PASP and the combination of PGLU/PASP formed stable mineralization solutions, and yielded nano-structured composites with the highest mineral content. Such studies contribute to our goal of preparing biomimetic bone graft substitutes with composition and structure that mimic bone.
Spontaneous Enhancement of Packing Regularity of Spherical Microdomains in the Body-Centered Cubic Lattice upon Uniaxial Stretching of Elastomeric Triblock Copolymers
Authors: Kota Takuya , Imaizumi Kimio , Sasaki Sono , Sakurai Shinichi
Published: December 2010

Block copolymers forming glassy spheres in the matrix of rubbery chains can exhibit elastomeric properties. It is well known that the spherical microdomains are arranged in the body-center cubic (bcc) lattice. However, recently, we have found packing in the face-centered cubic (fcc) lattice, which is easily transformed into the bcc lattice upon uniaxial stretching. In the same time, the packing regularity of the spheres in the bcc lattice was found to be enhanced for samples completely recovered from the stretched state. This reminds us that a cycle of stretching-and-releasing plays an important role from analogy of densification of the packing in granules upon shaking. In the current paper, we quantify the enhancement of packing regularity of spherical microdomains in the bcc lattice upon uniaxial stretching of the same elastomeric triblock copolymer as used in our previous work by conducting small-angle X-ray scattering (SAXS) measurements using high brilliant synchrotron radiation. Isotropically circular rings of the lattice peaks observed for the unstretched sample turned into deformed ellipsoidal rings upon the uniaxial stretching, with sharpening of the peaks in the direction parallel to the stretching direction and almost disappearing of the peaks in the perpendicular direction. By quantitatively analyzing the SAXS results, it was found that the packing regularity of the spherical microdomains was enhanced in the parallel direction while it was spoiled in the perpendicular direction under the stretched state. The enhanced regularity of packing was unchanged even if the stretching load was completely removed.
Conduction and Electrostriction of Polymers Induced by High Electric Fields
Authors: Karahka Markus , Kreuzer Hans Jürgen
Published: December 2010

After reviewing the new physics and chemistry in high electrostatic fields we use density functional theory to show that in fields around 1.5 V/Å the bandgap of polythiophene reduces to zero leading to field-induced metallization. In poly(ethylene glycol), on the other hand, such fields lead to giant electrostriction of over 20% in length. Lastly, we give two examples of field-induced polymerization: (1) the closure of sulfur molecules Sn at n = 8 is suppressed remaining linear up to n ~ 20. (2) This also happens to water which forms linear whiskers up to n ~ 11.
Degradable Poly(ester amide)s for Biomedical Applications
Authors: Rodriguez-Galan Alfonso , Franco Lourdes , Puiggali Jordi
Published: December 2010

Poly(ester amide)s are an emerging group of biodegradable polymers that may cover both commodity and speciality applications. These polymers have ester and amide groups on their chemical structure which are of a degradable character and provide good thermal and mechanical properties. In this sense, the strong hydrogen‑bonding interactions between amide groups may counter some typical weaknesses of aliphatic polyesters like for example poly(e-caprolactone). Poly(ester amide)s can be prepared from different monomers and following different synthetic methodologies which lead to polymers with random, blocky and ordered microstructures. Properties like hydrophilic/hydrophobic ratio and biodegradability can easily be tuned. During the last decade a great effort has been made to get functionalized poly(ester amide)s by incorporation of a-amino acids with hydroxyl, carboxyl and amine pendant groups and also by incorporation of carbon-carbon double bonds in both the polymer main chain and the side groups. Specific applications of these materials in the biomedical field are just being developed and are reviewed in this work (e.g., controlled drug delivery systems, hydrogels, tissue engineering and other uses like adhesives and smart materials) together with the main families of functionalized poly(ester amide)s that have been developed to date.
Chitosan-Based Hyaluronic Acid Hybrid Polymer Fibers as a Scaffold Biomaterial for Cartilage Tissue Engineering
Authors: Iwasaki Norimasa , Kasahara Yasuhiko , Yamane Shintarou , Igarashi Tatsuya , Minami Akio , Nisimura Shin-ichiro
Published: December 2010

An ideal scaffold material is one that closely mimics the natural environment in the tissue-specific extracellular matrix (ECM). Therefore, we have applied hyaluronic acid (HA), which is a main component of the cartilage ECM, to chitosan as a fundamental material for cartilage regeneration. To mimic the structural environment of cartilage ECM, the fundamental structure of a scaffold should be a three-dimensional (3D) system with adequate mechanical strength. We structurally developed novel polymer chitosan-based HA hybrid fibers as a biomaterial to easily fabricate 3D scaffolds. This review presents the potential of a 3D fabricated scaffold based on these novel hybrid polymer fibers for cartilage tissue engineering.
Flooding Effect on Earth Walls
Authors: Medero Gabriela M. , Kennedy Justin H. , Woodward Peter K. , Banimahd Meysam
Published: December 2010

Earth building is a sustainable, environmentally friendly and economical method of construction that has been used worldwide for many centuries. For the past three decades, earth has seen a revival as a building material for a modern construction method due to its benefits in terms of low carbon content, low cost and energy involved during construction, as well as the fact that it is a sustainable technology of building. Climate change is influencing precipitation levels and patterns around the world, and as a consequence, flood risk is increasing rapidly. When flooding occurs, earth buildings are exposed to water by submersion, causing an increase in the degree of saturation of the earth structures and therefore a decrease of the suction between particles. This study investigated the effect of cycles of flooding (consecutive events of flooding followed by dry periods) on earth walls. A series of characterization tests were carried out to obtain the physical and mechanical properties of the studied earth material. In a second stage, Flooding Simulation Tests (FST) were performed to explore the earth walls’ response to repeated flooding events. The results obtained for the tested earth wall/samples with reinforced material (straw) reveal hydraulic hysteresis when wall/samples are subject to cycles of wetting and drying.
Review of Temephos Discriminating Concentration for Monitoring the Susceptibility of Anopheles labranchiae (Falleroni, 1926), Malaria Vector in Morocco
Authors: Faraj C. , Adlaoui E. , Elkohli M. , Herrak T. , Ameur B. , Chandre F.
Published: December 2010

In Morocco, the resistance monitoring of Anopheles labranchiae larvae to temephos is done using discriminating concentration of 0.125 mg, which is half of the WHO recommended dose for Anopheles. However, this dosage seemed to be too high to allow an early detection of the resistance and its revision was found necessary. The present study was carried out during May-June 2008 and 2009 in nine provinces from the north-west of the country. The aim was to determine the lethal concentrations LC100 of temephos for the most susceptible populations and to define the discriminating dosage as the double of this value. The bioassays were conducted according to WHO standard operating protocol to establish the dose-mortality relationship and deduct the LC50 and LC95. The results of this study indicated that the LC100 obtained on the most susceptible populations was close to 0.05 mg/l. Therefore, the temephos discriminating dosage for susceptibility monitoring of An. labranchiae larvae in Morocco was set to be 0.1 mg/l.
Alzheimer's Disease: A Pathogenetic Autoimmune Disorder Caused by Herpes Simplex in a Gene-Dependent Manner
Authors: Carter C. J.
Published: December 2010

Herpes simplex is implicated in Alzheimer's disease and viral infection produces Alzheimer's disease like pathology in mice. The virus expresses proteins containing short contiguous amino acid stretches (5–9aa “vatches” = viralmatches) homologous to APOE4, clusterin, PICALM, and complement receptor 1, and to over 100 other gene products relevant to Alzheimer's disease, which are also homologous to proteins expressed by other pathogens implicated in Alzheimer's disease. Such homology, reiterated at the DNA level, suggests that gene association studies have been tracking infection, as well as identifying key genes, demonstrating a role for pathogens as causative agents. Vatches may interfere with the function of their human counterparts, acting as dummy ligands, decoy receptors, or via interactome interference. They are often immunogenic, and antibodies generated in response to infection may target their human counterparts, producing protein knockdown, or generating autoimmune responses that may kill the neurones in which the human homologue resides, a scenario supported by immune activation in Alzheimer's disease. These data may classify Alzheimer's disease as an autoimmune disorder created by pathogen mimicry of key Alzheimer's disease-related proteins. It may well be prevented by vaccination and regular pathogen detection and elimination, and perhaps stemmed by immunosuppression or antibody adsorption-related therapies.
Online Intelligent Controllers for an Enzyme Recovery Plant: Design Methodology and Performance
Authors: Leite M. S. , Fujiki T. L. , Silva F. V. , Fileti A. M. F.
Published: December 2010

This paper focuses on the development of intelligent controllers for use in a process of enzyme recovery from pineapple rind. The proteolytic enzyme bromelain (EC 3.4.22.4) is precipitated with alcohol at low temperature in a fed-batch jacketed tank. Temperature control is crucial to avoid irreversible protein denaturation. Fuzzy or neural controllers offer a way of implementing solutions that cover dynamic and nonlinear processes. The design methodology and a comparative study on the performance of fuzzy-PI, neurofuzzy, and neural network intelligent controllers are presented. To tune the fuzzy PI Mamdani controller, various universes of discourse, rule bases, and membership function support sets were tested. A neurofuzzy inference system (ANFIS), based on Takagi-Sugeno rules, and a model predictive controller, based on neural modeling, were developed and tested as well. Using a Fieldbus network architecture, a coolant variable speed pump was driven by the controllers. The experimental results show the effectiveness of fuzzy controllers in comparison to the neural predictive control. The fuzzy PI controller exhibited a reduced error parameter (ITAE), lower power consumption, and better recovery of enzyme activity.
Intracytoplasmic Crystalline Inclusions in the Hepatocytes of an Antelope
Authors: Gumber Sanjeev , Lombardini Eric , Williams Bruce , Wakamatsu Nobuko
Published: December 2010

This case report describes intracytoplasmic crystalline inclusions in the hepatocytes of a 13-year-old female Thomson's gazelle. Histologically, multifocal to coalescing areas of many hepatocytes contained large cytoplasmic vacuoles filled with pale eosinophilic homogeneous material and rare fine basophilic granules. Von Kossa staining showed the presence of calcium within cytoplasm, mainly in the inclusions, of hepatocytes. Transmission electron microscopy, scanning electron microscopy, energy dispersive X-rays analyses, and infrared spectroscopy on the liver showed the hepatocellular material consistent with protein and carbohydrate with secondary accumulation of calcium and phosphorus. It was concluded that crystalline inclusions may have been derived due to failure of normal physiological hepatocellular clearance associated with a severe chronic disease. To the authors' knowledge this is the first reported case of hepatocellular crystalline inclusions in an antelope.
Potential Applications of Immobilizedβ-Galactosidase in Food Processing Industries
Authors: Panesar Parmjit S. , Kumari Shweta , Panesar Reeba
Published: December 2010

The enzymeβ-galactosidase can be obtained from a wide variety of sources such as microorganisms, plants, and animals. The use ofβ-galactosidase for the hydrolysis of lactose in milk and whey is one of the promising enzymatic applications in food and dairy processing industries. The enzyme can be used in either soluble or immobilized forms but the soluble enzyme can be used only for batch processes and the immobilized form has the advantage of being used in batch wise as well as in continuous operation. Immobilization has been found to be convenient method to make enzyme thermostable and to prevent the loss of enzyme activity. This review has been focused on the different types of techniques used for the immobilization ofβ-galactosidase and its potential applications in food industry.
Brain Imaging of Nicotinic Receptors in Alzheimer's Disease
Authors: Wu Jin , Ishikawa Masatomo , Zhang Jichun , Hashimoto Kenji
Published: December 2010

Neuronal nicotinic acetylcholine receptors (nAChRs) are a family of ligand-gated ion channels which are widely distributed in the human brain. Several lines of evidence suggest that two major subtypes (α4β2 and α7) of nAChRs play an important role in the pathophysiology of Alzheimer's disease (AD). Postmortem studies demonstrated alterations in the density of these subtypes of nAChRs in the brain of patients with AD. Currently, nAChRs are one of the most attractive therapeutic targets for AD. Therefore, several researchers have made an effort to develop novel radioligands that can be used to study quantitatively the distribution of these two subtypes in the human brain with positron emission tomography (PET) and single-photon emission computed tomography (SPECT). In this paper, we discuss the current topics onin vivoimaging of two subtypes of nAChRs in the brain of patients with AD.
Effect of Ochratoxin A on Body Weight, Feed Intake and Feed Conversion in Broiler Chicken
Authors: Sakthivelan Sigamani Masilamani , Sudhakar Rao Ganne Venkata
Published: December 2010

The effect of ochratoxin A (OA) on the body weight, feed intake, and feed conversion was investigated in broiler chicken fed dietary levels of OA at 0, 1, and 2 ppm for 28 days from hatch. Feeding OA significantly reduced the growth rate of broiler chicken. The reduction was observed from the first week onwards in OA-treated groups. Feed consumption and feed conversion also showed a diminishing trend from the first week of feeding toxin. Its implication on the performance of broiler chicken is discussed.
New Proteins Found Interacting with Brain Metallothionein‐3 Are Linked to Secretion
Authors: El Ghazi Issam , Martin Bruce L. , Armitage Ian M.
Published: December 2010

Metallothionein 3 (MT‐3), also known as growth inhibitory factor (GIF), exhibits a neuroinhibitory activity. Our lab and others have previously shown that this biological activity involves interacting protein partners in the brain. However, nothing specific is yet known about which of these interactions is responsible for the GIF activity. In this paper, we are reporting upon new proteins found interacting with MT‐3 as determined through immunoaffinity chromatography and mass spectrometry. These new partner proteins—Exo84p, 14‐3‐3 Zeta, α and β Enolase, Aldolase C, Malate dehydrogenase, ATP synthase, and Pyruvate kinase—along with those previously identified have now been classified into three functional groups: transport and signaling, chaperoning and scaffolding, and glycolytic metabolism. When viewed together, these interactions support a proposed model for the regulation of the GIF activity of MT‐3.
Ceruloplasmin/Transferrin Ratio Changes in Alzheimer′s Disease
Authors: Squitti Rosanna , Salustri Carlo , Siotto Mariacristina , Ventriglia Mariacarla , Vernieri Fabrizio , Lupoi Domenico , Cassetta Emanuele , Rossini Paolo M.
Published: December 2010

The link between iron and Alzheimer′s disease (AD) has been mainly investigated with a focus on the local accumulation of this metal in specific areas of the brain that are critical for AD. In the present study, we have instead looked at systemic variations of markers of iron metabolism. We measured serum levels of iron, ceruloplasmin, and transferrin and calculated the transferrin saturation and the ceruloplasmin to transferrin ratio (Cp/Tf). Cp/Tf and transferrin saturation increased in AD patients. Cp/Tf ratios also correlated positively with peroxide levels and negatively with serum iron concentrations. Elevated values of ceruloplasmin, peroxides, and Cp/Tf inversely correlated with MMSE scores. Isolated medial temporal lobe atrophy positively correlated with Cp/Tf and negatively with serum iron. All these findings indicate that the local iron accumulation found in brain areas critical for AD should be viewed in the frame of iron systemic alterations.
Association of Inflammatory and Oxidative Stress Markers with Metabolic Syndrome in Asian Indians in India
Authors: Rao Veena S. , Nagaraj Radhika K. , Hebbagodi Sridhara , Kadarinarasimhiah Natesha B. , Kakkar Vijay V.
Published: December 2010

Metabolic syndrome (MetS) is a primary risk factor for cardiovascular disease and is associated with a proinflammatory state. Here, we assessed the contribution of inflammatory and oxidative stress markers towards prediction of MetS. A total of 2316 individuals were recruited in Phase I of the Indian Atherosclerosis Research Study (IARS). Modified ATPIII guidelines were used for classification of subjects with MetS. Among the inflammatory and oxidative stress markers studied, levels of hsCRP (P<.0001), Neopterin (P=.036), and oxLDL (P<.0001) were significantly higher among subjects with MetS. Among the markers we tested, oxLDL stood out as a robust predictor of MetS in the IARS population (OR 4.956 95% CI 2.504–9.810;P<.0001) followed by hsCRP (OR 1.324 95% CI 1.070–1.638;P=.010). In conclusion, oxLDL is a candidate predictor for MetS in the Asian Indian population.