bluuebunny/crossref_metadata_2025 huggingface dataset:
РОЗВИТОК ЦИФРОВОЇ КОМПЕТЕНТНОСТІ МАЙБУТНІХ УЧИТЕЛІВ АНГЛІЙСЬКОЇ МОВИ З ВИКОРИСТАННЯМ ПРОГРАМНОГО ЗАСОБУ STORYJUMPER
Authors: Хомишак Оксана Богданівна , Патієвич Ольга Василівна
Published: October 2020

Ретроспективний аналіз свідчить, що традиційно процес навчання відбувався в межах тріади «учитель – учень – підручник». Однак науково-технічний прогрес ХХI століття додав до цієї взаємодії таку важливу ланку, як цифрові технології. Упродовж тривалого часу паперовий підручник уважався основним засобом навчання, а вчитель – єдиним джерелом знань. Проте сьогодні до взаємодії учителя та учня як суб’єктів освітнього процесу на уроці активно долучаються цифрові технології, оскільки дають можливість миттєвого доступу до всебічної інформації та наукового знання, що невпинно нагромаджується та збагачується. З огляду на це професійне становлення майбутнього вчителя іноземної мови без володіння цифровою компетентністю, що охоплює впевнене, критичне та відповідальне використання і взаємодію з цифровими технологіями для навчання, роботи та участі в житті суспільства, неможливе. Отже, комп'ютеризація та інформатизація освіти зумовлюють пошук нових підходів до фахової підготовки майбутнього вчителя, зокрема англійської мови. У статті розкрито зміст програми навчальної дисципліни «Сучасні технології навчання англійської мови у закладах загальної середньої освіти (ЗЗСО)» з циклу професійної підготовки студентів напряму «6.02030302 Філологія. «Мова і література (англійська)» Дрогобицького державного педагогічного університету імені Івана Франка (2017/2018 навчальний рік). На основі набутого педагогічного досвіду обґрунтовано доцільність формування цифрової компетентності майбутнього вчителя англійської мови у процесі його фахової підготовки. Проілюстровано результати вивчення вище вказаної дисципліни на прикладі студентської проєктної роботи, презентованої як фрагмент електронного посібника як доповнення до чинного підручника з англійської мови для 1-го класу ЗЗСО. Проаналізовано фрагмент електронного посібника та доведено, що програмa-шаблон дає змогу майбутньому вчителеві англійської мови самотужки укладати, розробляти, структурувати і систематизувати мовні та мовленнєві завдання, наповнювати контент мультимедійним матеріалом (аудіо-, відео-, статичною і / або динамічною анімацією) за умови дотримання певних лінгвометодичних, психолого-педагогічних та ергономічних параметрів. Унаслідок опитування студентів встановлено, що цифрові технології удосконалюють процес навчання іноземної мови завдяки миттєвості доступу до всебічної, професійно значущої та аутентичної інформації, мультимедійності, креативності, інтерактивності та можливості міжкультурної взаємодії, що забезпечує досягнення освітніх цілей, а цифрова компетентність є невід’ємною складовою частиною професійної підготовки майбутнього вчителя англійської мови в сучасних умовах.
ІНТЕЛЕКТ-КАРТА ЯК ЗАСІБ СИСТЕМАТИЗАЦІЇ ЗНАНЬ З ФОРМУВАННЯ ПРОФЕСІЙНОГО ІМІДЖУ МАГІСТРІВ ПЕДАГОГІКИ ВИЩОЇ ШКОЛИ
Authors: Коркішко Артем Володимирович , Коркішко Олена Геннадіївна , Саяпіна Світлана Анатоліївна
Published: October 2020

Статтю присвячено проблемі застосування в навчальному процесі вищої школи інтерактивного способу систематизації знань щодо формування професійного іміджу магістрів педагогіки вищої школи – інтелект-карт. Зазначено, що використання інтелект-карт під час вивчення курсу «Професійний імідж викладача вищої школи» зараз актуальне, оскільки робить процес навчання творчим і захоплюючим, дозволяє вирішити низку педагогічних завдань іміджевого спрямування, виконати роботу науковим способом і більш ефективно, наочно представити великий обсяг інформації, спростити аналіз даних та їх запам’ятовування, справлятися з інформаційним потоком, керувати та структуризувати його, використовуючи весь свій творчий потенціал; актуалізує певний комплекс знань, який необхідно засвоїти при вивченні курсу, забезпечує інтенсифікацію навчання з урахуванням зорового сприйняття, образного уявлення знань і навчальних дій, підвищує іміджеву грамотність і візуальну культуру, а також вдало поєднує навчальну, аналітичну діяльність, що, безумовно, є діяльним і ефективним у реалізації сучасних завдань системи освіти. Розкрито етапи роботи щодо створення інтелект-карти «Структура формування професійного іміджу викладача»: теоретичний блок (виділення структурних компонентів професійного іміджу викладача, ознайомлення з концепцією радіантного мислення та практичними рекомендаціями із складання інтелект-карт, обговорення основних правил створення інтелект-карти), практичний блок (апробація – складання інтелект-карти «Структура формування професійного іміджу викладача» магістрами ПВШ, аналіз – презентація інтелект-карт студентами, обговорення їхнього змісту та оформлення, рефлексія – самоаналіз діяльності). Зроблено висновок про те, що використання інтелект-карт дозволяє визначити власні цілі та життєві цінності, розпочати шлях безперервного самоаналізу протягом життя, усвідомити власні сильні й слабкі сторони як фахівця, розробити план із самовдосконалення професійного іміджу.
USING BLOOM’S REVISED TAXONOMY TO DEVELOP DIGITAL LITERACIES IN THE ESP CLASSROOM
Authors: Вавіліна Світлана Геннадіївна
Published: October 2020

The article explores some issues to be considered when integrating new technologies into English for Specific Purposes (ESP) learning. The main focus is on the challenges that ESP practitioners face in the context of a multilingual digital Europe. In confronting such problems as changing roles for students and teachers, the spread of English as a medium of instruction and increased emphasis on the subject content in the language classroom, it is necessary for ESP teachers to embrace innovation and develop strategies to improve students’ learning. To benefit from the use of technology, ESP teachers need to concentrate on those aspects which digital knowledge and skills share with traditional literacies. When essential elements of digital literacies are singled out, it becomes possible to employ their full potential for creating a technology enhanced learning environment. This research suggests doing it by drawing upon the Taxonomy of Educational Objectives. The framework, in this case, is used to check that goals falling within different categories of the cognitive domain of learning are aligned with technology-supported instruction delivery methods. While designing instructional techniques, we heavily rely on the idea of scaffolding in the form of visual aids for enabling learners to build on prior knowledge and internalize new concepts. As practical examples show, development of digital literacies proves not only to be compatible with formation of professional intercultural communicative competence but also providing means for activating the most complex cognitive processes of conceptual understanding, critical thinking, decision making, creation and metacognition. The findings may be useful in preparing instructional activities with the help of technological tools to support ESP teaching and learning.
MOBILE MEDIATED LEARNING IN LANGUAGE CLASSROOMS: LEARNER’S SATISFACTION, THEIR PERCEIVED USEFULNESS OF INSTRUCTION AND CLASSROOM ACHIEVEMENT
Authors: Азізізі Марал , Разсай Ехсан , Багері Мохаммад Садег
Published: October 2020

Mobile technology has recently become one of the most important fields of study for many educators in the field of teaching and learning. Mobile technologies are considered as valuable resources for language learning and teaching and provide many practical applications for language learning. The concept of mobile mediated learning and its underlying constructs along with their implications for language learning and teaching are little understood in Iranian context. So, it is important to investigate how different aspects of mobile mediated learning including omnipresence, context customization, interactivity, perceived self-efficacy, and m-learning motivation affect second language learning. This paper investigates the understanding of mobile mediated language learning among Iranian English as a Foreign Language (EFL) learners by examining the effect of different aspects of this concept on learners’ satisfaction with mobile learning as well as the learners’ perception of usefulness of mobile learning. Moreover, this study aims to examine the effect of learners’ perceived satisfaction and perceived usefulness of mobile learning on EFL learners' classroom achievement. To this end, a sample of 150 EFL learners were added to a Telegram group for 12 mobile-assisted language learning sessions and then answered three questionnaires regarding aspects of mobile mediated learning and also learners’ perceived satisfaction and perceived usefulness of mobile learning. This study has a proposed research model that shows the constructs examined in this study. Structural equation modeling results indicated that two aspects of mobile mediated learning had a significant effect on learner’s perceived satisfaction. The findings also showed that three aspects of mobile mediated learning affected learner’s perceived usefulness of mobile learning. The results revealed that learner’s classroom achievement is not influenced by learners’ perceived satisfaction and perceived usefulness of mobile learning.
ЦИФРОВІ ОСВІТНІ ХАБИ ДЛЯ ПІДТРИМКИ ГРОМАДЯНСЬКОЇ ОСВІТИ ЯК СКЛАДОВА ІНФОРМАЦІЙНО-ЦИФРОВОГО НАВЧАЛЬНОГО СЕРЕДОВИЩА: ДОСВІД НІДЕРЛАНДІВ, БЕЛЬГІЇ ТА УКРАЇНИ
Authors: Гриценчук Олена Олександрівна
Published: October 2020

У статті висвітлюється міжнародний та вітчизняний досвід впровадження цифрових освітніх хабів для підтримки громадянської освіти як елементу інформаційно-цифрового навчального середовища. Проаналізовано основні поняття, розглянуто теоретичні підходи вітчизняних і зарубіжних дослідників та визначено роль цифрового освітнього хабу як сучасного інструменту інформаційно-цифрового навчального середовища. Обґрунтовано поняття «цифровий освітній хаб», що є багатофункціональним цифровим освітнім простором, де зібрано електронні освітні ресурси, навчальні матеріали, інструменти, технології; забезпечуються організаційно-педагогічні умови для набуття знань, формування та розвитку компетентностей учасників освітнього процесу, створено умови для ефективної та оперативної акумуляції інтелектуального потенціалу, що сприяє професійному та особистому самовдосконаленню, створенню інновацій та їх впровадженню в освіту. Опрацьовано вітчизняну законодавчу базу, що визначає актуальність громадянської освіти та потенціал цифрових освітніх хабів. На основі досвіду Нідерландів і Бельгії описано інструменти цифрових освітніх хабів, засобами яких реалізується громадянська освіта та її елементи в освітньому процесі закладів загальної середньої освіти. Здійснено аналіз сучасного стану впровадження освітніх хабів у вітчизняній освітній практиці та виявлено проблеми, що потребують ґрунтовного дослідження. Практичне значення цифрового освітнього хабу як складової інформаційно-цифрового навчального середовища полягає у сприянні створенню інновацій та їх упровадженню в освіту; реалізації проєктів; формуванні та розвитку особистості сучасного громадянина, що активно діє у цифровому середовищі. Новизна дослідження полягає в тому, що на основі узагальнення вітчизняного та зарубіжного досвіду надано рекомендації вітчизняним фахівцям щодо створення ресурсного цифрового освітнього хабу для підтримки громадянської освіти.
Authors: Suprianto Suprianto , Budiarsa I Made
Published: October 2020

Penyebaran pandemi COVID-19 telah menyita banyak perhatian masyarakat global. Pandemi ini disebabkan oleh SARS-CoV-2 dengan memproduksi protein nonstruktural (nsp1-16). Penelitian ini bertujuan untuk menganalisis struktur tersier nsp1 dan nsp2 pada SARS-CoV-2 secara in-silico. Struktur tersier model dibangun melalui metode homologi, urutan asam amino nsp1 dan nsp2 yang digunakan memiliki kode akses YP_009725297.1 dan YP_009725298.1 yang diperoleh dari NCBI. Komposisi asam amino nsp1 dan nsp2 masing-masing adalah memiliki 19774.35 Daltons dan 70507.81 Daltons. Struktur tersier nsp1 dibangun menggunakan template d2gdta1 dan kepercayaan modeling 100% menghasilkan struktur model dengan persentase 64%, wilayah disordered 19%, alpha-helix 42%, beta strand 13%, verifikasi model 92.17% (lulus), 74.0% favoured region dan daerah outliers 6.4% (stabil). Struktur tersier nsp2 dibangun menggunakan template c2lc3A dan kepercayaan modelling 47.1% menghasilkan struktur model dengan persentase 9%, wilayah disordered 17%, alpha-helix 36%, beta strand 27%, verifikasi model 64.29% (gagal), 76.0% favoured region dan daerah outliers 16.4% (tidak stabil). Stabilitas nsp1 memiliki potensi yang sangat baik dibandingkan nsp2 untuk dikembangkan lebih lanjut sebagai vaksin. Validasi struktur yang diperoleh dapat menjadi dasar dalam mempelajari pengembangan desain vaksin virus SARS-CoV-2 sebagai salah satu penyebab penyakit COVID-19.
Perbandingan Metode RT-PCR dan Tes Rapid Antibodi untuk Deteksi COVID-19
Authors: Agustina Anita Suswanti , Fajrunni'mah Rizana
Published: October 2020

COVID-19 is caused by SARS-CoV-2, which can spread rapidly from human to human. There are several laboratory tests to detect COVID-19, including the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method and a rapid test antibody test to detect antibody reactions to SARS-CoV-2. Both methods have advantages and disadvantages of each, while the literature study comparing the two methods is currently limited. The type of research used is library research. Research materials have been collected from various journals, books, and guidelines, in line with the research topic, to obtain 24 library sources. The results of the literature study indicate that the target genes that can be used to detect COVID-19 RT-PCR methods include the N, E, RdRp, and ORF1a/b genes. The sensitivity of rapid antibody tests is known to range from 68−89%, while the specificity of rapid antibody tests ranges from 91−100%. RT-PCR has the advantage of being able to detect low-concentration antigens, but RT-PCR has weaknesses such as requiring expensive equipment and inspection fees, specially trained laboratory personnel, long working time, and high risk of exposure. Rapid antibody testing has advantages, including ease of sampling, lower testing costs, reduced risk of exposure to officers, does not require special equipment and space, but has the potential for cross-reactivity with other coronaviruses. Both the RT-PCR method and the rapid antibody test have their advantages and disadvantages, but rapid antibody testing with RT-PCR can improve the diagnosis of COVID-19. The results of this literature study are expected to be continued as a basis for further research on RT-PCR examination and antibody rapid test for COVID-19 detection in Indonesia, accompanied by information on onset time and time-testing with a large sample of research.
Optimisation of Casting Geometries for A356 Alloy Composites Reinforced with Organic Materials using Box-Behnken Design Methodology
Authors: Oke Sunday , Nwafor Stephen Chidera , Ayanladun Chris Abiodun
Published: October 2020

In an earlier article, the central composite design was applied to the determination of geometrical features of casts in a two-phase transformation process to produce the wheel covers of automobiles whereby the A356 alloy is reinforced with organic substances for composite property enhancement. This article reexamines the assumptions in that circumstance to revise and expand the optimisation through the response surface methodology to a new method, Box-Behnken design (BBD), to facilitate a comprehensive treatment of the sand casting product parameters. Casting geometrical optimisation can be modelled to involve lengths, breadths, widths, heights, densities of casts and weight loss, varied at three discrete levels. The parameters are translated into codes (–1,0,1) with specified actual, minimum and maximum values. The framework, validated by published literature data, indicates its feasibility in a real-life circumstance. This article assessed the effects of the casting geometry parameters on the responses. Besides, it examined the accuracy of the parameters to predict in the regression models deployed. It was concluded that the BBD and the regression models are adequate and predict correctly. The BBD can be applied by composite developers to improve casting dimensional accuracy and economics.
INCREASING THE YIELD OF RED CLOVER SEEDS DUE TO ACTIVATING THE POLLINATION FUNCTION OF BEES
Authors: Zaryanova Zoya
Published: October 2020

The development of animal husbandry and the transfer of agriculture to a biological basis requires an in-crease in the area of crops of perennial leguminous grasses. For the conditions of the Northern part of the Central Black Earth region of the Russian Federation, meadow clover is one of the most popular crops. In the Orel region over the past 10 years, meadow clover in its pure form and in grass mixtures occupied an average of 41.8% in the structure of perennial grasses. For more efficient economic use of clover, it is ne-cessary to improve the provision of farms with its seeds, which requires the development and implemen-tation of new environmentally friendly methods of cultivation technology. Red clover is a strictly cross-pollinated, entomophilous plant. Natural self-pollination does not exceed 1–3%. The main pollinators of meadow clover are currently honeybees, which perform up to 90% of the pollination work on the produc-tion sowings of this crop. To increase seed gathering, the effectiveness of the drug Pollinus use, which increases the attractiveness of meadow clover as a honey plant, was established. The treatment of seed herbage with the Pollinus attractant allowed to increase the seed yield by 20.1 kg/ha (+21.2% to the con-trol). Due to the intensification of the flight-pollination activity of bees, the seed set in treated area was 65.1%, the semination of heads inflorescences – 58.9%, which exceeded the control (untreated area) by 16.9 and 15.9%, respectively. The use of attractant was economically profitable, since the price of seed crop increase is higher than additional costs by 2500–3500 rubles/ha.
EFFECT OF CROP ROTATION WITH LUPINE AND BASIC SOIL CULTIVATION METHODS ON WEED INFESTATION OF SOWING IN BRYANSK REGION
Authors: Isaeva Elena , YaGOVENKO German , Pedosich Olga
Published: October 2020

Tests have been done on gray forest soil in the South-West of the Non-Chernozem zone of Bryansk re-gion in the stationary experiment in the All-Russian Lupin Research Institute in 2015–2018. The aim of the test was to evaluate the effect of the intensity of the basic soil cultivation on crop weed infestation in lupin crop rotation. The experiment has been done during a rotation of the field crop rotation (winter wheat – bare oats – winter triticale – lupin) turned in time and space in the fields of white lupin and win-ter wheat where lupin has been cultivated as a predecessor. During the test years 7 weeds species have been fixed at tillering phase of winter wheat, its number made 9.4–15.4 units/m2. The number of the main weeds decreased to 11 species at the end of the vegetation season (at ripeness stage) and made 9.7–15.9 units/m2. There have been noticed 8 weeds species in white lupin crops, the number made 33.6–55.8 units/m2. The deep non-moldboard soil cultivation decreased weeds number in crop rotation. At dif-ferent basic soil cultivation 8 weeds species occurred in crops. The others were either in the deep non-moldboard soil cultivation or in the surface one. The number and ratio of weeds species changed when the grass component increased to the final field of crop rotation. The annual dicotyledonous species made 45.0–60.5% decreasing at the surface cultivation. Perennial weeds made 38.5–55.0% of the total weeds coenosis. Their number decreased in the case of deep cultivation. Crop infestation degree and as a result the weeds twice during the rotation at the surface soil cultivation.
PENINGKATAN KECERDASAN BODY KINESTHETIC MELALUI KEGIATAN TARI KREATIF (Penelitian Tindakan di Kelompok B TK/RA Daaruttuqo, Cipondoh)
Authors: Rosita Rosita
Published: October 2020

Objectives of the research are to determine the creative dance and to increase body kinesthetic intelligence of childrenÂ  through creative dance. This research was carried outÂ  in group B Daaruttuqo kindergarten, Cipondoh Tangerang withÂ  10 subjectÂ , on the month from May to June. This research uses an action research design model of Kemmis and Mc Taggart, through two cycles consisting of seven meetings on the first cycle and seven meetings on the second cycle which includes the stages of planning, action, observation and reflection. Data analysis using quantitative and qualitative analysis. Data obtained by referring to the research instrument which refers to the body kinesthetic intelligence. The results showedÂ  an increase in body kinesthetic intelligence scores of children with the acquisition of body kinesthetic intelligence classical pre-action reached 37.5%, the first cycle increased the score reached 56.75% and the second cycle was obtained which satisfy the classical score reached 80.75 % so it can be concluded that theÂ  activityâ€creative danceâ€ can improve children's body kinesthetic intelligence.
What Do You Think About Your Company’s Leaks? A Survey on End-Users Perception Toward Data Leakage Mechanisms
Authors: Bertrand Yoann , Boudaoud Karima , Riveill Michel
Published: October 2020

Data leakage can lead to severe issues for a company, including financial loss, damage of goodwill, reputation, lawsuits and loss of future sales. To prevent these problems, a company can use other mechanisms on top of traditional Access Control. These mechanisms include for instance Data Leak Prevention or Information Rights Management and can be referred as Transmission Control. However, such solutions can lack usability and can be intrusive for end-users employees. To have a better understanding of the perception and usage of such mechanisms within business infrastructures, we have conducted in this article an online survey on 150 employees. These employees come from different companies of different sizes and sectors of activity. The results show that whatever the size of the company or its sector of activity, security mechanisms such as access control and transmission control can be considered as quite intrusive and blocking for employees. Moreover, our survey also shows interesting results regarding more acceptable and user-friendly anti-data leakage mechanisms that could be used within companies.
The Role of Autologous Stem-Cell Transplantation in High-Risk Neuroblastoma Consolidated by anti-GD2 Immunotherapy. Results of Two Consecutive Studies
Authors: Mora Jaume , Castañeda Alicia , Flores Miguel A. , Santa-María Vicente , Garraus Moira , Gorostegui Maite , Simao Margarida , Perez-Jaume Sara , Mañe Salvador
Published: October 2020

Background: Treatment of HR-NB comprise induction, consolidation with autologous stem cell transplant (ASCT) followed by anti-GD2 immunotherapy and isotretinoin. Childrens Oncology Group and SIOPEN studies used dinutuximab and cytokines to treat patients in complete remission or refractory Bone/Bone marrow (B/BM) disease after ASCT.

Methods: HR-NB patients referred to Hospital Sant Joan de Déu for anti-GD2 immunotherapy were eligible for two consecutive studies (dinutuximab for EudraCT 2013–004864–69 and naxitamab for 017–001829–40) and naxitamab/Sargramostim CU with or without prior ASCT. Patients enrolled in first complete remission or with primary refractory B/BM disease. We accrued a study population of two groups whose therapy, aside from ASCT, was similar. This is a retrospective analysis of their outcome calculated from study entry.

Results: From December 2014–2019, 67 patients were treated with dinutuximab and cytokines (n = 21) in the Hospital Sant Joan de Déu-HRNB-Ch14.18 study or with naxitamab and Sargramostim either in the Ymabs study 201 (n = 12) or CU (n = 34). 23 patients were treated with primary refractory disease in the B/BM (11 with dinutuximab and 12 with naxitamab), and 44 in first CR (10 with dinutuximab and 34 with naxitamab). Study patients included 13 (19.4%) treated following single ASCT and 54 following conventional chemotherapy. Median follow-up for all patients is 16.2 months. Two-year rates for ASCT and non-ASCT patients were, respectively, EFS 64.1% vs. 54.2% (p = 0.28), and OS 66.7% vs. 84.1% (p = 0.81). For the 44 pts in first CR, 2-years rates for ASCT and non-ASCT patients were, respectively, EFS 65.5% vs. 58.7% (p = 0.48), and OS 71.4% vs. 85.4% (p = 0.63).

Conclusions: In this retrospective, single center study, ASCT did not provide survival benefit when anti-GD2 immunotherapy was used after induction chemotherapy.
Yield Performance and Response to High Plant Densities of Dry Bean (Phaseolus vulgaris L.) Cultivars under Semi-Arid Conditions
Authors: Baez-Gonzalez Alma Delia , Fajardo-Diaz Ricardo , Padilla-Ramirez Jose Saul , Osuna-Ceja Esteban Salvador , Kiniry James R. , Meki Manyowa N. , Acosta-Díaz Efraín
Published: October 2020

To identify eco-efficient bean cultivars that can be planted at high densities for sustainable bean production under climate change, this study analyzed the performance of ten dry bean (Phaseolus vulgaris L.) cultivars grown at 90,000, 145,000 and 260,000 plants ha−1 under rainfed semi-arid conditions in Mexico. The study compared the yield and yield components (leaf area index (LAI), pods per plant, and hundred seed weight) of the cultivars. We also analyzed the dry matter distribution (DMD), growth rate (GR), radiation use efficiency (RUE), and harvest index (HI) of the best performing cultivars to determine how they respond to higher densities. The cultivars were established under similar planting and management conditions during two growing seasons. The precipitation for the first and second seasons were 175 and 492 mm, respectively, representing 57% and 160% of the mean precipitation in the area during the July–October growing period. Pinto Saltillo, a drought-tolerant indeterminate semi-prostrate cultivar, and Azufrado 2, a determinate shrub cultivar, performed best at high densities under low-precipitation conditions (175 mm). Both cultivars responded to the highest density (260,000 plants ha−1) with increases of 54% to 69% (0.7 to 1.1) in LAI and 21% to 86% (0.32–0.81 Mg ha−1) in yield. The two cultivars responded to increasing plant density with a modification in their fraction of DMD over plant parts and a change in their GR from 0.23–0.25 at low density to 0.96–1.74 gr m−2 day−1 at high density. The two cultivars had an RUE of 3.8 to 4.4 g MJ−1 and HI of 0.31 to 0.36 at high planting density. Farmers’ use of these commercially available cultivars proven to have high yields and the ability to respond favorably to high densities under rainfed conditions can be a viable short-term strategy to increase dry bean production for sustainable agriculture in semi-arid temperate regions.
Assessment of Landsat-Based Evapotranspiration Using Weighing Lysimeters in the Texas High Plains
Authors: Hashem Ahmed A. , Engel Bernard A. , Bralts Vincent F. , Marek Gary W. , Moorhead Jerry E. , Radwan Sherif A. , Gowda Prasanna H.
Published: October 2020

Evapotranspiration (ET) is one of the largest data gaps in water management due to the limited availability of measured evapotranspiration data, and because ET spatial variability is difficult to characterize at various scales. Satellite-based ET estimation has been shown to have great potential for water resource planning and for estimating agricultural water use at field, watershed, and regional scales. Satellites with low spatial resolution, such as NASA’s MODIS (Moderate Resolution Imaging Spectroradiometer), and those with higher spatial resolution, such as Landsat (Land Satellite), can potentially be used for irrigation water management purposes and other agricultural applications. The objective of this study is to assess satellite based-ET estimation accuracy using measured ET from large weighing lysimeters. Daily, seven-day running average, monthly, and seasonal satellite-based ET data were compared with corresponding lysimeter ET data. This study was performed at the USDA-ARS Conservation and Production Research Laboratory (CPRL) in Bushland, Texas, USA. The daily time series Landsat ET estimates were characterized as poor for irrigated fields, with a Nash Sutcliff efficiency (NSE) of 0.37, and good for monthly ET, with an NSE of 0.57. For the dryland managed fields, the daily and monthly ET estimates were unacceptable with an NSE of −1.38 and −0.19, respectively. There are various reasons for these results, including uncertainties with remotely sensed data due to errors in aerodynamic resistance surface roughness length estimation, surface temperature deviations between irrigated and dryland conditions, poor leaf area estimation in the METRIC model under dryland conditions, extended gap periods between satellite data, and using the linear interpolation method to extrapolate daily ET values between two consecutive scenes (images).
Effects of Feeding Dried Fruit Pomaces as Additional Fibre-Phenolic Compound on Meat Quality, Blood Chemistry and Redox Status of Broilers
Authors: Colombino Elena , Zduńczyk Zenon , Jankowski Jan , Cocolin Luca Simone , Schiavone Achille , Biasato Ilaria , Prieto-Botella Daniel , Karlińska Elzbieta , Kosmala Monika , Ognik Katarzyna , Capucchio Maria Teresa , Juśkiewicz Jerzy
Published: October 2020

The present study investigated the effects of apple (A), blackcurrant (B) and strawberry (S) dried pomaces on meat quality, blood chemistry and redox status of broiler chickens. A total of 480 Ross-308 male broilers were divided into 8 dietary treatments containing 3% and 6% of cellulose preparation (C), A, B or S. Six birds/group were slaughtered at 35 days of age and blood samples were collected. Carcass traits and meat quality were determined on the Pectoralis major muscles, recording nonsignificant differences. Antioxidant activity was evaluated in serum, liver and breast muscle. In serum, fruit pomaces lowered triglycerides, creatinine and atherogenic index (p < 0.05). Regarding redox status, in serum, ACW (antioxidant capacity of water-soluble substances) and ACL (antioxidant capacity of lipid-soluble substances) were greater in A (p < 0.001). In breast, ACW and ACL were higher in B and S compared to C (p < 0.05). In liver, ACL was greater in B and S compared to C (p < 0.001) and in higher dosage compared to low (p = 0.036). GSSG (oxidized glutathione) concentration was lower in A, whereas A, B and S presented a higher GSH (reduced glutathione)/GSSG ratio. The results showed that fruit pomaces could represent promising feed ingredients for broilers, improving serum, meat and tissue antioxidant parameters.
Phylogenetic Relationships of Turkish Indigenous Donkey Populations Determined by Mitochondrial DNA D-loop Region
Authors: Özkan Ünal Emel , Özdil Fulya , Kaplan Selçuk , Gürcan Eser Kemal , Genç Serdar , Arat Sezen , Soysal Mehmet İhsan
Published: October 2020

In this study, to analyze the mtDNA D-loop region and the origin of the maternal lineages of 16 different donkey populations, and to assess the domestication of Turkish indigenous donkeys in seven geographical regions, we investigated the DNA sequences of the D-loop region of 315 indigenous donkeys from Turkey. A total of 54 haplotypes, resulting from 35 polymorphic regions (27 parsimoniously informative and 6 singleton sites), were defined. Twenty-eight of these haplotypes are unique (51.85%), and 26 are shared among different Turkish indigenous donkey populations. The most frequent haplotype was Hap 1 (45.71%), followed by two haplotypes (Hap 4, 15.55% and Hap 7, 5.39%). The breed genetic diversity, evaluated by the haplotype diversity (HD) and nucleotide diversity (πD), for the Turkish donkey populations ranged from 0.533 ± 0.180 (Tekirdağ–Malkara, MAL) to 0.933 ± 0.122 (Aydin, AYD), and from 0.01196 ± 0.0026 (Antalya, ANT) to 0.02101 ± 0.0041 (Aydin, AYD), respectively. We observed moderate-to-high levels of haplotype diversity and moderate nucleotide diversity, indicating plentiful genetic diversity in all of the Turkish indigenous donkey populations. Phylogenetic analysis (NJT) and median-joining network analysis established that all haplotypes were distinctly grouped into two major haplogroups. The results of AMOVA analyses, based on geographic structuring of Turkish native donkey populations, highlighted that the majority of the observed variance is due to differences among samples within populations. The observed differences between groups were found to be statistically significant. Comparison among Turkish indigenous donkey mtDNA D-loop regions and haplotypes, and different countries’ donkey breeds and wild asses, identified two clades and which is named Somali (Clade IV) and Nubian (Clade V) lineages. The results can be used to understand the origin of Turkish donkey populations clearly, and to resolve the phylogenetic relationship among all of the different regions.
Validation of the Equine Behaviour Assessment and Research Questionnaire (E-BARQ): A New Survey Instrument for Exploring and Monitoring the Domestic Equine Triad
Authors: Fenner Kate , Matlock Sarah , Williams Jane , Wilson Bethany , McLean Andrew , Serpell James , McGreevy Paul
Published: October 2020

The Equine Behaviour Assessment and Research Questionnaire (E-BARQ) was developed to obtain quantitative data on the domestic equine triad: training, management and behaviour. It can be taken repeatedly, thus collecting longitudinal data to enable evaluation of how changes in a horse’s training and management are reflected in that horse’s behaviour over time and how these changes can impact horse welfare in the longer term. Questionnaire validation and reliability were tested by determining (a) whether an owner’s subjective ratings of their horse’s problematic behaviours or undesirable temperament traits were reflected in the questionnaire scores obtained for that horse (construct validity), (b) whether two respondents, equally familiar with a particular horse, reported comparable scores for that horse through the questionnaire (inter-rater reliability), and (c) whether the same respondent, scoring the same horse after a known interval of time, recorded similar responses (intra-rater reliability). Construct validity testing of 1923 responses showed significant alignment between owners’ reported experience of focal horses’ behaviour and those horses’ E-BARQ scores, with scores varying from 1.13 to 1.34 for ridden horse behaviour (all p < 0.001) and from 1.06 to 1.43 for non-ridden horse behaviour (all p < 0.001). Inter-rater reliability testing of ten horse–rider pairs revealed that 203 of the 215 question items were significantly aligned (p < 0.001) when tested by two independent raters. Of the remaining 19 items, four had fair alignment (ĸ = 0.174–0.316; p = 0.281) and ten items, largely related to whether the horse shows behavioural signs related to anxiety when taken away from home, did not align (ĸ = 0; p = 1). Intra-rater reliability tests showed that the responses significantly aligned on all 215 question items tested (p < 0.001). The results of these tests confirmed the construct validity and reliability of E-BARQ as a standardised behavioural assessment tool for horses.
Impact of High Temperature on Post-Exercise Albuminuria in Dogs
Authors: Pasławska Urszula , Szczepankiewicz Barbara , Bednarska Aleksandra , Pasławski Robert
Published: October 2020

The literature suggests that strenuous exercise and exposure to high temperatures may cause physiologic proteinuria, but to our knowledge there have been no studies that have assessed the effect of high temperatures on the occurrence of post-exercise albuminuria in dogs. The goal of the study was to assess the impact of high temperatures on the occurrence of albuminuria. Thirteen healthy adult dogs—eight female (62%) and five male (38%) had to run 5 km at a temperature of 25 °C in grasslands which took about 30–40 min. Dogs underwent clinical examinations: echocardiography, abdominal ultrasound, blood hematology and biochemistry and urinalysis, including the ratio of albumin to creatinine (UAC). Baseline UAC was on the borderline of statistical significance for female dogs, but not for male dogs, before and after exercise. UAC was 0.31 ± 0.56 mg/mmol for female dogs and 0.36 ± 0.60 mg/mmol in male dogs before exercise. Immediately after exercise, UAC was 0.51 ± 0.58 mg/mmol in female dogs and 0.31 ± 0.40 mg/mmol in male dogs. Thus, a period of about 30–40 min of intensive exercise at high temperatures (25 °C) did not lead to increased albuminuria. This suggests that there is no need to limit the movement activity before urine tests in dogs, even at high temperatures, before urinalysis.
Optimization of CASA-Mot Analysis of Donkey Sperm: Optimum Frame Rate and Values of Kinematic Variables for Different Counting Chamber and Fields
Authors: Gacem Sabrina , Catalán Jaime , Valverde Anthony , Soler Carles , Miró Jordi
Published: October 2020

In order to optimize the donkey sperm motility analysis by the CASA (Computer Assisted Sperm Analysis)-Mot system, twelve ejaculates were collected from six jackasses. Capillary loaded chamber (CLC), ISAS®D4C depths 10 and 20 µm, ISAS®D4C Leja 20 and drop displacement chamber (DDC), Spermtrack® (Spk) depths 10 and 20 µm were used. Sperm kinematic variables were evaluated using each chamber and a high-resolution camera capable of capturing a maximum of 500 frames/second (fps). The optimum frame rate (OFR) (defined according to curvilinear velocity—VCL) was dependent on chamber type. The highest OFR obtained was 278.46 fps by Spk20. Values for VCL, straight-line velocity (VSL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were high in DDC and 10 µm depth. In both DDC 10 and 20 µm, the sperm velocities (VCL, VSL, VAP) and ALH values decreased significantly from the centre to the edges, while Wobble and BCF increased. No defined behavior was observed along the CLC. However, all the kinematic variables had a higher value in a highly concentrated sample, in both chamber types. In conclusion, analyzing a minimum of nine fields at 250 fps from the centre to the edges in Spk10 chamber using a dilution of 30 × 106 sperm/mL offers the best choice for donkey computerised sperm motility analysis.
Impact of Goji Berries (Lycium barbarum) Supplementation on the Energy Homeostasis of Rabbit Does: Uni- and Multivariate Approach
Authors: Menchetti Laura , Curone Giulio , Andoni Egon , Barbato Olimpia , Troisi Alessandro , Fioretti Bernard , Polisca Angela , Codini Michela , Canali Claudio , Vigo Daniele , Brecchia Gabriele
Published: October 2020

This study examined the effects of goji berries dietary supplementation on the energetic metabolism of doe. Thirty days before artificial insemination, 75 New Zealand White does were assigned to three different diets: commercial standard diet (C) and supplemented with 1% (LG) and 3% (HG) of goji berries, respectively. Body conditions, hormones and metabolites were monitored until weaning. Body weight and BCS were higher in HG than C (p < 0.05). LG showed lower T3/T4 ratio and cortisol concentrations (p < 0.05) and tended to have lower indices of insulin resistances (p < 0.1) than HG. Compared to control, leptin was higher in HG at AI (p < 0.01) and in LG during lactation (p < 0.05). Two principal components were extracted by multivariate analysis describing the relationships between (1) non-esterified fatty acids, insulin and glucose levels, and (2) body conditions and leptin metabolism. The first component highlighted the energy deficit and the insulin resistance of the does during pregnancy and lactation. The second one showed that leptin, body weight and Body Condition Score (BCS) enhance as levels of goji berries in the diet increase. Thus, the effects of goji supplementation are dose-dependent: an improvement on energy metabolism was achieved with a low-dose while the highest dose could determine excessive fattening and insulin resistance in does.
Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database
Authors: Almeida Daniela , Domínguez-Pérez Dany , Matos Ana , Agüero-Chapin Guillermin , Osório Hugo , Vasconcelos Vitor , Campos Alexandre , Antunes Agostinho
Published: October 2020

Cephalopods, successful predators, can use a mixture of substances to subdue their prey, becoming interesting sources of bioactive compounds. In addition to neurotoxins and enzymes, the presence of antimicrobial compounds has been reported. Recently, the transcriptome and the whole proteome of the Octopus vulgaris salivary apparatus were released, but the role of some compounds—e.g., histones, antimicrobial peptides (AMPs), and toxins—remains unclear. Herein, we profiled the proteome of the posterior salivary glands (PSGs) of O. vulgaris using two sample preparation protocols combined with a shotgun-proteomics approach. Protein identification was performed against a composite database comprising data from the UniProtKB, all transcriptomes available from the cephalopods’ PSGs, and a comprehensive non-redundant AMPs database. Out of the 10,075 proteins clustered in 1868 protein groups, 90 clusters corresponded to venom protein toxin families. Additionally, we detected putative AMPs clustered with histones previously found as abundant proteins in the saliva of O. vulgaris. Some of these histones, such as H2A and H2B, are involved in systemic inflammatory responses and their antimicrobial effects have been demonstrated. These results not only confirm the production of enzymes and toxins by the O. vulgaris PSGs but also suggest their involvement in the first line of defense against microbes.
Membrane-Targeting Triphenylphosphonium Functionalized Ciprofloxacin for Methicillin-Resistant Staphylococcus aureus (MRSA)
Authors: Kang Sangrim , Sunwoo Kyoung , Jung Yuna , Hur Junho K. , Park Ki-Ho , Kim Jong Seung , Kim Dokyoung
Published: October 2020

Multidrug-resistant (MDR) bacteria have become a severe problem for public health. Developing new antibiotics for MDR bacteria is difficult, from inception to the clinically approved stage. Here, we have used a new approach, modification of an antibiotic, ciprofloxacin (CFX), with triphenylphosphonium (TPP, PPh3) moiety via ester- (CFX-ester-PPh3) and amide-coupling (CFX-amide-PPh3) to target bacterial membranes. In this study, we have evaluated the antibacterial activities of CFX and its derivatives against 16 species of bacteria, including MDR bacteria, using minimum inhibitory concentration (MIC) assay, morphological monitoring, and expression of resistance-related genes. TPP-conjugated CFX, CFX-ester-PPh3, and CFX-amide-PPh3 showed significantly improved antibacterial activity against Gram-positive bacteria, Staphylococcus aureus, including MDR S. aureus (methicillin-resistant S. aureus (MRSA)) strains. The MRSA ST5 5016 strain showed high antibacterial activity, with MIC values of 11.12 µg/mL for CFX-ester-PPh3 and 2.78 µg/mL for CFX-amide-PPh3. The CFX derivatives inhibited biofilm formation in MRSA by more than 74.9% of CFX-amide-PPh3. In the sub-MIC, CFX derivatives induced significant morphological changes in MRSA, including irregular deformation and membrane disruption, accompanied by a decrease in the level of resistance-related gene expression. With these promising results, this method is very likely to combat MDR bacteria through a simple TPP moiety modification of known antibiotics, which can be readily prepared at clinical sites.
Towards Flexible Integration of 5G and IIoT Technologies in Industry 4.0: A Practical Use Case
Authors: Sasiain Jorge , Sanz Ane , Astorga Jasone , Jacob Eduardo
Published: October 2020

The Industry 4.0 revolution envisions fully interconnected scenarios in the manufacturing industry to improve the efficiency, quality, and performance of the manufacturing processes. In parallel, the consolidation of 5G technology is providing substantial advances in the world of communication and information technologies. Furthermore, 5G also presents itself as a key enabler to fulfill Industry 4.0 requirements. In this article, the authors first propose a 5G-enabled architecture for Industry 4.0. Smart Networks for Industry (SN4I) is introduced, an experimental facility based on two 5G key-enabling technologies—Network Functions Virtualization (NFV) and Software-Defined Networking (SDN)—which connects the University of the Basque Country’s Aeronautics Advanced Manufacturing Center and Faculty of Engineering in Bilbao. Then, the authors present the deployment of a Wireless Sensor Network (WSN) with strong access control mechanisms into such architecture, enabling secure and flexible Industrial Internet of Things (IIoT) applications. Additionally, the authors demonstrate the implementation of a use case consisting in the monitoring of a broaching process that makes use of machine tools located in the manufacturing center, and of services from the proposed architecture. The authors finally highlight the benefits achieved regarding flexibility, efficiency, and security within the presented scenario and to the manufacturing industry overall.
EPF—An Efficient Forwarding Mechanism in SDN Controller Enabled Named Data IoTs
Authors: Tariq Asadullah , Rehman Rana Asif , Kim Byung-Seo
Published: October 2020

The vision of the Internet of Things (IoT) is that it connects all kinds of things by leveraging the creation of increasingly affordable and small devices that can be embedded for sensing, processing, wireless communication, and actuation. Named data networking (NDN) is a newly emerging Internet paradigm that may replace the current Internet architecture and that fulfills most of the expectations of the IoT. Software-defined networking (SDN) is an emerging paradigm of technology that is highly capable of managing overall networks efficiently and transforming complex network architectures into manageable, simple ones. The combination of the SDN controller, NDN, and IoT can be lethal in the overall performance of the network. Broadcast storms, due to the flooding nature of NDN’s wireless channel, are a serious issue when it comes to forwarding interest and data packets. Energy consumption of sensor nodes in dense IoT scenarios causes problems in forwarding as well as unnecessary delays, decreases network performance, and increases the cost and packet delay for important packets. We took these problems as our baseline and proposed an energy-efficient, priority-based forwarding (EPF) in SDN-enabled NDN–IoT. Our scheme EPF used the efficient flow management of the SDN controller to control the broadcast storm and efficiently forward the priority-based packets. A defer timer mechanism was used to prioritized the packet upon its arrival to the node. An energy threshold mechanism was used to control energy consumption and improve overall energy efficiency. We compared our scheme with the traditional flooding mechanism and geographic interest forwarding; EPF outclassed the other schemes and produced the best results in terms of total number of interests and retransmissions, content retrieval time, total number of priority interests, energy consumption, and network lifetime.