bluuebunny/crossref_metadata_2025 huggingface dataset:
Impaired ketogenesis is associated with metabolic-associated fatty liver disease in subjects with type 2 diabetes
Authors: Lee Sejeong , Bae Jaehyun , Jo Doo Ri , Lee Minyoung , Lee Yong-ho , Kang Eun Seok , Cha Bong-Soo , Lee Byung-Wan
Published: February 2023

Aims

The ketogenic pathway is an effective mechanism by which the liver disposes of fatty acids (FAs) to the peripheral tissues. Impaired ketogenesis is presumed to be related to the pathogenesis of metabolic-associated fatty liver disease (MAFLD), but the results of previous studies have been controversial. Therefore, we investigated the association between ketogenic capacity and MAFLD in subjects with type 2 diabetes (T2D).

Methods

A total of 435 subjects with newly diagnosed T2D was recruited for the study. They were classified into two groups based on median serum β-hydroxybutyrate (β-HB) level: intact vs. impaired ketogenesis groups. The associations of baseline serum β-HB and MAFLD indices of hepatic steatosis index, NAFLD liver fat score (NLFS), Framingham Steatosis index (FSI), Zhejian University index, and Chinese NAFLD score were investigated.

Results

Compared to the impaired ketogenesis group, the intact ketogenesis group showed better insulin sensitivity, lower serum triglyceride level, and higher low-density lipoprotein-cholesterol and glycated hemoglobin levels. Serum levels of liver enzymes were not different between the two groups. Of the hepatic steatosis indices, NLFS (0.8 vs. 0.9, p=0.045) and FSI (39.4 vs. 47.0: p=0.041) were significantly lower in the intact ketogenesis group. Moreover, intact ketogenesis was significantly associated with lower risk of MAFLD as calculated by FSI after adjusting for potential confounders (adjusted odds ratio 0.48, 95% confidence interval 0.25-0.91, p=0.025).

Conclusions

Our study suggests that intact ketogenesis might be associated with decreased risk of MAFLD in T2D.
Identification of biomarkers and prediction of upstream miRNAs in diabetic nephropathy
Authors: Yin Dapeng , Guo Zhixin , Zhang Xinyu
Published: February 2023

Objective

To explore biomarkers of diabetic nephropathy (DN) and predict upstream miRNAs.

Methods

The data sets GSE142025 and GSE96804 were obtained from Gene Expression Omnibus database. Subsequently, common differentially expressed genes (DEGs) of renal tissue in DN and control group were identified and protein-protein interaction network (PPI) was constructed. Hub genes were screened from in DEGs and made an investigation on functional enrichment and pathway research. Finally, the target gene was selected for further study. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficiency of target gene and predicted its upstream miRNAs.

Results

130 common DEGs were obtained through analysis, and 10 Hub genes were further identified. The function of Hub genes was mainly related to extracellular matrix (ECM), collagen fibrous tissue, transforming growth factor (TGF) -β, advanced glycosylation end product (AGE) -receptor (RAGE) and so on. Research showed that the expression level of Hub genes in DN group was significantly higher than that in control group. (all P<0.05). The target gene matrix metalloproteinase 2 (MMP2) was selected for further study, and it was found to be related to the fibrosis process and the genes regulating fibrosis. Meanwhile, ROC curve analysis showed that MMP2 had a good predictive value for DN. miRNA prediction suggested that miR-106b-5p and miR-93-5p could regulate the expression of MMP2.

Conclusion

MMP2 can be used as a biomarker for DN to participate in the pathogenesis of fibrosis, and miR-106b-5p and miR-93-5p may regulate the expression of MMP2 as upstream signals.
Early Holocene plant macrofossils indicate cool refugia for subalpine plant taxa in Acadia National Park, Maine
Authors: Carroll Cas F. , Gill Jacquelyn L. , MacKenzie Caitlin McDonough
Published: February 2023

Identifying refugia— specifically places where species can persist during periods of regionally unsuitable climate— is increasingly important for conservation practitioners and land managers charged with protecting biodiversity in a rapidly warming world. Currently, many researchers assist in this process by building models to predict areas of refugia using climate data projected into the future under different climate scenarios; however, the coarse spatial scale of future climate data can be orders of magnitude larger than the scale of refugia on the landscape. Conservation paleobiology is an emerging field that can contribute to the identification of climate refugia by looking at the macrofossil records contained in sediments to better understand the response of species to past climate change within a small area, and allows us to ground-truth hypotheses about specific areas functioning as climate refugia. Here, we present a conservation paleobiology case study to update vulnerability assessments for subalpine plant species in Acadia National Park and locate potential future refugia on the landscape. We analyzed plant macrofossils in a sediment core from Sargent Mountain Pond in Acadia National Park (Maine, United States) at a fine spatiotemporal resolution to test the hypothesis that the area served as a past climate refugium for the subalpine species it currently hosts. We found that, when compared to a pollen record from a forest hollow core collected on Mount Desert Island, the macrofossils reflect a more stable presence of subalpine taxa throughout the Holocene Climatic Optimum (8,000–5,000 BP) than was observed at lower elevations. Our results indicate the importance of a complementary approach that combines plant macrofossils and pollen in addition to modeling to identify refugia and better understand the vulnerability of species and communities to climate change.
Genome-wide analysis of R2R3-MYB transcription factors in Boehmeria nivea (L.) gaudich revealed potential cadmium tolerance and anthocyanin biosynthesis genes
Authors: Feng Xinkang , Abubakar Aminu Shehu , Chen Kunmei , Yu Chunming , Zhu Aiguo , Chen Jikang , Gao Gang , Wang Xiaofei , Mou Pan , Chen Ping
Published: February 2023

Gene family, especially MYB as one of the largest transcription factor family in plants, the study of its subfunctional characteristics is a key step in the study of plant gene function. The sequencing of ramie genome provides a good opportunity to study the organization and evolutionary characters of the ramie MYB gene at the whole genome level. In this study, a total of 105 BnGR2R3-MYB genes were identified from ramie genome and subsequently grouped into 35 subfamilies according to phylogeny divergence and sequences similarity. Chromosomal localization, gene structure, synteny analysis, gene duplication, promoter analysis, molecular characteristics and subcellular localization were accomplished using several bioinformatics tools. Collinearity analysis showed that the segmental and tandem duplication events is the dominant form of the gene family expansion, and duplications prominent in distal telomeric regions. Highest syntenic relationship was obtained between BnGR2R3-MYB genes and that of Apocynum venetum (88). Furthermore, transcriptomic data and phylogenetic analysis revealed that BnGMYB60, BnGMYB79/80 and BnGMYB70 might inhibit the biosynthesis of anthocyanins, and UPLC-QTOF-MS data further supported the results. qPCR and phylogenetic analysis revealed that the six genes (BnGMYB9, BnGMYB10, BnGMYB12, BnGMYB28, BnGMYB41, and BnGMYB78) were cadmium stress responsive genes. Especially, the expression of BnGMYB10/12/41 in roots, stems and leaves all increased more than 10-fold after cadmium stress, and in addition they may interact with key genes regulating flavonoid biosynthesis. Thus, a potential link between cadmium stress response and flavonoid synthesis was identified through protein interaction network analysis. The study thus provided significant information into MYB regulatory genes in ramie and may serve as a foundation for genetic enhancement and increased productivity.
Relationship between early lung adenocarcinoma and multiple driving genes based on artificial intelligence medical images of pulmonary nodules
Authors: Yin Yajun , Lu Jiawei , Tong Jichun , Cheng Youshuang , Zhang Ke
Published: February 2023

Lung adenocarcinoma is one of the most common cancers in the world, and accurate diagnosis of lung nodules is an important factor in reducing its mortality. In the diagnosis of pulmonary nodules, artificial intelligence (AI) assisted diagnosis technology has been rapidly developed, so testing its effectiveness is conducive to promoting its important role in clinical practice. This paper introduces the background of early lung adenocarcinoma and lung nodule AI medical imaging, and then makes academic research on early lung adenocarcinoma and AI medical imaging, and finally summarizes the biological information. In the experimental part, the relationship analysis of 4 driver genes in group X and group Y showed that there were more abnormal invasive lung adenocarcinoma genes, and the maximum uptake value and uptake function of metabolic value were also higher. However, there was no significant correlation between mutations in the four driver genes and metabolic values, and the average accuracy of AI-based medical images was 3.88% higher than that of traditional images.
Compression hemostasis using fully covered self-expandable metallic stents for refractory hemorrhages caused by esophageal cancer: A pilot study
Authors: Bi Yonghua , Ren Jianzhuang , Han Xinwei
Published: February 2023

Objective

Fully covered self-expandable metallic stents (SEMSs) have been widely used as a salvage therapy for patients with esophageal variceal bleeding. However, the role of fully covered SEMSs in the management of hemorrhage caused by esophageal cancer has not yet been established. We aimed to investigate the safety and efficacy of fully covered SEMSs as a salvage therapy for esophageal cancer-related hemorrhage.

Methods

From September 2019 to March 2022, 17 patients, who underwent the insertion of fully covered SEMS for malignant esophageal hemorrhages, were retrospectively analyzed. Chest computed tomography (CT) scans and esophagographies were performed routinely to determine the location and length of the tumor. A fully covered SEMS was implanted under fluoroscopy. Baseline demographics were retrospectively collected, that is those for sex, age, previous treatment, comorbidities, lesion type, and stent size.

Results

A total of 20 metal stents were placed in 17 patients, with a technical success rate of 100% and a hemostasis success rate of 88.2%. Stent removal was performed in three patients because of complications. No perioperative deaths were related to stent placement or removal. Five main complications (29.4%) were found after stent insertion. Stent migration and restenosis were observed in two patients (11.8%). Except for two perioperative deaths and one patient lost to follow-up, all remaining 14 patients were successfully followed up. At the end of follow-up, two patients had survived without obvious symptoms, and a total of 12 patients were dead owing to tumor progression (n = 10), severe infection (n = 1), and cerebrovascular accident (n = 1). The median overall survival was 13.8 months.

Conclusion

Insertion of a fully covered SEMS may be a safe and effective means of the salvage management of refractory esophageal cancer-related hemorrhage, and its use in this context may lead to the development of innovative methods for compression hemostasis. However, further study with a larger sample size and comparison with other forms of salvage therapy.
C9 immunostaining as a tissue biomarker for periprosthetic joint infection diagnosis
Authors: Meinshausen Ann-Kathrin , Färber Jacqueline , Illiger Sebastian , Macor Paolo , Lohmann Christoph H. , Bertrand Jessica
Published: February 2023

Background

Culture-negative periprosthetic joint infections (PJI) are often false diagnosed as aseptic implant failure leading to unnecessary revision surgeries due to repeated infections. A marker to increase the security of e PJI diagnosis is therefore of great importance. The aim of this study was to test C9 immunostaining of periprosthetic tissue as a novel tissue-biomarker for a more reliable identification of PJI, as well as potential cross-reactivity.

Method

We included 98 patients in this study undergoing septic or aseptic revision surgeries. Standard microbiological diagnosis was performed in all cases for classification of patients. Serum parameters including C-reactive protein (CRP) serum levels and white blood cell (WBC) count were included, and the periprosthetic tissue was immunostained for C9 presence. The amount of C9 tissue staining was evaluated in septic versus aseptic tissue and the amount of C9 staining was correlated with the different pathogens causing the infection. To exclude cross-reactions between C9 immunostaining and other inflammatory joint conditions, we included tissue samples of a separate cohort with rheumatoid arthritis, wear particles and chondrocalcinosis.

Results

The microbiological diagnosis detected PJI in 58 patients; the remaining 40 patients were classified as aseptic. Serum CRP values were significantly increased in the PJI cohort. Serum WBC was not different between septic and aseptic cases. We found a significant increase in C9 immunostaining in the PJI periprosthetic tissue. To test the predictive value of C9 as biomarker for PJI we performed a ROC analyses. According to the Youden’s criteria C9 is a very good biomarker for PJI detection with a sensitivity of 89% and a specificity of 75% and an AUC of 0.84. We did not observe a correlation of C9 staining with the pathogen causing the PJI. However, we observed a cross reactivity with the inflammatory joint disease like rheumatoid arthritis and different metal wear types. In addition, we did not observe a cross reactivity with chondrocalcinosis.

Conclusion

Our study identifies C9 as a potential tissue-biomarker for the identification of PJI using immunohistological staining of tissue biopsies. The use of C9 staining could help to reduce the number of false negative diagnoses of PJI.
Self-assembled nanoparticles: A new platform for revolutionizing therapeutic cancer vaccines
Authors: Shi Tianyu , Sun Mengna , Lu Changchang , Meng Fanyan
Published: February 2023

Cancer vaccines have had some success in the past decade. Based on in-depth analysis of tumor antigen genomics, many therapeutic vaccines have already entered clinical trials for multiple cancers, including melanoma, lung cancer, and head and neck squamous cell carcinoma, which have demonstrated impressive tumor immunogenicity and antitumor activity. Recently, vaccines based on self-assembled nanoparticles are being actively developed as cancer treatment, and their feasibility has been confirmed in both mice and humans. In this review, we summarize recent therapeutic cancer vaccines based on self-assembled nanoparticles. We describe the basic ingredients for self-assembled nanoparticles, and how they enhance vaccine immunogenicity. We also discuss the novel design method for self-assembled nanoparticles that pose as a promising delivery platform for cancer vaccines, and the potential in combination with multiple therapeutic approaches.
Third COVID-19 vaccine dose for people with multiple sclerosis who did not seroconvert following two doses of BBIBP-CorV (Sinopharm) inactivated vaccine: A pilot study on safety and immunogenicity
Authors: Sedaghat Nahad , Etemadifar Masoud , Lotfi Noushin , Sayahi Farnaz , Chitsaz Ahmad , Salari Mehri , Ghasemi Movaghar Alireza
Published: February 2023

Background

People with multiple sclerosis (pwMS) on anti-CD20 therapies (aCD20) and fingolimod have shown inadequate humoral responses to COVID-19 vaccines.

Objective

The objective of the study was to pilot larger studies by demonstrating the safety and comparing the immunogenicity of different types of third doses in seronegative pwMS after two doses of BBIBP-CorV inactivated vaccine.

Methods

In December 2021, subject to receiving their third dose, being COVID-19-naiive, and receiving no corticosteroid within two months, we measured the level of anti-SARS-CoV-2-Spike IgG in pwMS seronegative after two shots of BBIBP-CorV inactivated vaccine.

Results

We included 20/29 pwMS who received adenoviral vector (AV), 7/29 who received inactivated, and 2/29 who received conjugated third doses. No serious adverse events were reported two weeks post-third dose. The pwMS receiving AV third doses showed significantly increased IgG concentrations, while only the ones not on aCD20 and fingolimod responded to inactivated third doses. An ordinal logistic multivariable generalized linear model indicated that age (per year β: −0.10, P = 0.04), type of disease-modifying therapy (aCD20 β: −8.36, P <0.01; fingolimod β: −8.63, P = 0.01; others: reference), and type of third dose (AV or conjugated β: 2.36, P = 0.02; inactivated: reference) are predictive of third dose immunogenicity among pwMS who remain seronegative after two shots of BBIBP-CorV vaccine. Statistical significance was not achieved for variables sex, MS duration, EDSS, duration of DMT, duration of third dose to IgG test, and duration from last aCD20 infusion to third dose.

Conclusion

This preliminary pilot study highlights the need for further research to determine the optimal COVID-19 third dose vaccination strategy for pwMS living in areas where BBIBP-CorV vaccine has been used.
An ultra-high performance concrete incorporating viscosity-controlling agent: Fiber distribution and microstructure
Authors: Zhao Baojun , Zhang Zongjun , Wu Chen , Zou Changgen , Xu Xin , Yang Han , Zhang Wenqi
Published: February 2023

In order to realize the stability of steel fiber inside ultra-high performance Concrete (UHPC) under vibration and improve the use efficiency of fiber, bentonite is used as auxiliary cementitious material in this study. The influence of bentonite dosage on the mechanical properties of UHPC matrix and the mechanical properties and microstructure of ultra-high Performance fiber Reinforced Concrete (UHPFRC) is explored. On this basis, the functional relationships between the distribution and orientation of steel fibers, the freshness of the matrix and the rheological parameters of UHPC are established, and the evaluation model of the service efficiency of steel fibers is established. The results show that with the gradual increase of bentonite incorporation, the use efficiency of UHPFRC steel fiber increases first and then decreases, and when the cement content of bentonite was 2.5%, 5.0%, 7.5% and 10.0%, the flexural strength of UHPFRC increased by 9.0%, 17.6%, 18.5% and 6.1%, respectively. In addition, the increase of bentonite content will lead to the continuous decrease of the fluidity of fresh UHPC slurry (from 261 mm to 100 mm). When the bentonite content is 10.0%, the UHPC slurry has almost no fluidity (100 mm), which leads to the appearance of pores in the UHPC matrix and the decrease of compressive strength.
Efficacy of photodynamic therapy in the treatment of port wine stains: A systematic review and meta-analysis
Authors: Wang Ling , Li Lei , Huang Chao
Published: February 2023

Background

Port wine stains (PWS) often cause cosmetic effects and psychological distress. Pulsed dye lasers (PDL) and photodynamic therapy (PDT) are the most commonly used treatments. PDL is still the “gold standard” of therapy to date. However, its shortcomings have become apparent as clinical applications have increased. PDT has been proven as an alternative to PDL. Patients with PWS still lack enough evidence about PDT to make informed treatment decisions.

Objective

The purpose of this systematic review and meta-analysis was to assess the safety and effectiveness of PDT for PWS.

Methods

The online datasets, comprising PubMed, Embase, Web of Science, and the Cochrane Library, were searched for meta-analysis-relevant publications. Two reviewers separately evaluated the risk of bias in each listed study. Grading of Recommendations Assessment, Development, and Evaluation (GRADE) was used to assess the treatment and safety outcomes.

Results

Our search retrieved 740 hits and only 26 studies were finally included. Among the 26 studies included, 3 were randomized clinical trials, and 23 were prospective or retrospective cohort investigations. Based on a gathered assessment, the percentage of individuals achieving a 60% improvement was estimated to be 51.5% [95% confidence interval (CI): 38.7–64.1; I2 = 83.8%] and a ≥75% improvement was 20.5% (95% CI: 14.5–26.5; I2 = 78.2%) after 1–8.2 treatment sessions (GRADE score: very low). Due to the statistical diversity of the meta-analysis, a subgroup assessment was performed to determine the sources of diversity. The collected findings indicated that the impact of PDT on enhancing the medical effectiveness of PWS was significant in different treatment sessions, different types of ages, different locations of PWS, and different types of PWS. Pain and edema occurred in most patients. Hyperpigmentation was present in 7.9–34.1% of the patients in 17 studies. Photosensitive dermatitis, hypopigmentation, blister, and scar were infrequently reported, with 0–5.8% incidences.

Conclusion

Photodynamic therapy is recommended as a safe and effective treatment for PWS based on the current evidence. However, our findings are based on poor-quality evidence. Therefore, comparative investigations of a large scale and high quality are necessary to support this conclusion.
Mycoplasma genitalium infection in the female reproductive system: Diseases and treatment
Authors: Yu Jianwei , Zhou Yan , Luo Haodang , Su Xiaoling , Gan Tian , Wang Jingyun , Ye Zufeng , Deng Zhongliang , He Jun
Published: February 2023

Mycoplasma genitalium is a newly emerged sexually transmitted disease pathogen and an independent risk factor for female cervicitis and pelvic inflammatory disease. The clinical symptoms caused by M. genitalium infection are mild and easily ignored. If left untreated, M. genitalium can grow along the reproductive tract and cause salpingitis, leading to infertility and ectopic pregnancy. Additionally, M. genitalium infection in late pregnancy can increase the incidence of preterm birth. M. genitalium infections are often accompanied by co-infection with other sexually transmitted pathogens (Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis) and viral infections (Human Papilloma Virus and Human Immunodeficiency Virus). A recent study suggested that M. genitalium plays a role in tumor development in the female reproductive system. However, few studies endorsed this finding. In recent years, M. genitalium has evolved into a new “superbug” due to the emergence of macrolide-and fluoroquinolone-resistant strains leading to frequent therapy failures. This review summarizes the pathogenic characteristics of M. genitalium and the female reproductive diseases caused by M. genitalium (cervicitis, pelvic inflammatory disease, ectopic pregnancy, infertility, premature birth, co-infection, reproductive tumors, etc.), as well as its potential relationship with reproductive tumors and clinical treatment.
Comprehensive analysis of cuproptosis-related genes on bladder cancer prognosis, tumor microenvironment invasion, and drug sensitivity
Authors: Wang Honglei , Li Jinqiao , Zi Xiaolin , Yuan Xueli
Published: February 2023

Cuproptosis, a newly discovered form of programmed cell death, plays a vital role in the occurrence and development of tumors. However, the role of cuproptosis in the bladder cancer tumor microenvironment remains unclear. In this study, we developed a method for predicting the prognostic outcomes and guiding the treatment selection for patients with bladder cancer. We obtained 1001 samples and survival data points from The Cancer Genome Atlas database and Gene Expression Omnibus database. Using cuproptosis-related genes (CRGs) identified in previous studies, we analyzed CRG transcriptional changes and identified two molecular subtypes, namely high- and low-risk patients. The prognostic features of eight genes (PDGFRB, COMP, GREM1, FRRS1, SDHD, RARRES2, CRTAC1, and HMGCS2) were determined. The CRG molecular typing and risk scores were correlated with clinicopathological features, prognosis, tumor microenvironment cell infiltration characteristics, immune checkpoint activation, mutation burden, and chemotherapy drug sensitivity. Additionally, we constructed an accurate nomogram to improve the clinical applicability of the CRG_score. qRT-PCR was used to detect the expression levels of eight genes in bladder cancer tissues, and the results were consistent with the predicted results. These findings may help us to understand the role of cuproptosis in cancer and provide new directions for the design of personalized treatment and prediction of survival outcomes in patients with bladder cancer.
Clinical application of VATS combined with 3D-CTBA in anatomical basal segmentectomy
Authors: Zhang Lening , Wang Tuhui , Feng Yonggang , Chen Yizhao , Feng Chong , Qin Dongliang , Han Chunshan
Published: February 2023

Objective

This study aimed to summarize the clinical application experience of video-assisted thoracic surgery (VATS) combined with three-dimensional computed tomography-bronchography and angiography (3D-CTBA) in anatomical basal segmentectomy.

Methods

Clinical data of 42 patients who underwent bilateral lower sub-basal segmentectomy by VATS combined with 3D-CTBA in our hospital from January 2020 to June 2022 were retrospectively analyzed; the patients included 20 males and 22 females, with a median age of 48 (30–65) years. Combined with the preoperative enhanced CT and 3D-CTBA techniques to identify the altered bronchi, arteries, and veins during the operation, the anatomical resection of each basal segment of both lower lungs was completed through the fissure approach or inferior pulmonary vein approach.

Results

All operations were successfully completed without conversion to thoracotomy or lobectomy. The median operation time was 125 (90–176) min, the median intraoperative blood loss was 15 (10–50) mL, the median postoperative thoracic drainage time was 3 (2–17) days, and the median postoperative hospital stay was 5 (3–20) days. The median number of resected lymph nodes was 6 (5–8). There was no in-hospital death. Postoperative pulmonary infection occurred in 1 case, lower extremity deep vein thrombosis (DVT) in 3 cases, pulmonary embolism in 1 case, and persistent air leakage in the chest in 5 cases, all of which were improved by conservative treatment. Two cases of pleural effusion after discharge were improved after ultrasound guided drainage. Postoperative pathology showed 31 cases of minimally invasive adenocarcinoma, 6 cases of adenocarcinoma in situ (AIS), 3 cases of severe atypical adenomatous hyperplasia (AAH), and 2 cases of other benign nodules. All cases were lymph node-negative.

Conclusion

VATS combined with 3D-CTBA is safe and feasible in anatomical basal segmentectomy; consequently, this approach should be promoted and applied in clinical work.
Elevation of artemisinin content by co-transformation of artemisinin biosynthetic pathway genes and trichome-specific transcription factors in Artemisia annua
Authors: Hassani Danial , Taheri Ayat , Fu Xueqing , Qin Wei , Hang Liu , Ma Yanan , Tang Kexuan
Published: February 2023

Artemisinin, derived from Artemisia annua, is currently used as the first-line treatment for malaria. However, wild-type plants have a low artemisinin biosynthesis rate. Although yeast engineering and plant synthetic biology have shown promising results, plant genetic engineering is considered the most feasible strategy, but it is also constrained by the stability of progeny development. Here we constructed three independent unique overexpressing vectors harboring three mainstream artemisinin biosynthesis enzymes HMGR, FPS, and DBR2, as well as two trichomes-specific transcription factors AaHD1 and AaORA. The simultaneous co-transformation of these vectors by Agrobacterium resulted in the successful increase of the artemisinin content in T0 transgenic lines by up to 3.2-fold (2.72%) leaf dry weight compared to the control plants. We also investigated the stability of transformation in progeny T1 lines. The results indicated that the transgenic genes were successfully integrated, maintained, and overexpressed in some of the T1 progeny plants’ genomes, potentially increasing the artemisinin content by up to 2.2-fold (2.51%) leaf dry weight. These results indicated that the co-overexpression of multiple enzymatic genes and transcription factors via the constructed vectors provided promising results, which could be used to achieve the ultimate goal of a steady supply of artemisinin at affordable prices around the world.
“Out of office”: Availability norms and feeling burned out during the COVID-19 pandemic: The mediating role of autonomy and telepressure
Authors: Hendrikx Karolien , Van Ruysseveldt Joris , Proost Karin , van der Lee Sandra
Published: February 2023

Introduction

Digital innovations make it possible to work anywhere and anytime using any kind of device. Given these evolutions, availability norms are emerging at work. These norms specifically refer to the experienced beliefs or expectations from colleagues or superiors to be available for work-related communication after regular work hours. We rely on the Job-Demands Resources Model as we aim to investigate the relationship between these availability norms and burnout symptoms during the COVID-19 pandemic. We first of all study to what extent availability norms are associated with heightened burnout symptoms. Secondly, we study how both a personal demand, namely telepressure, and a job resource, namely autonomy, could offer distinct and relevant explanations for the role these availability norms play in experiencing burnout symptoms.

Method

We collected data through a survey study with 229 employees from various organizations in the second half of 2020.

Results

The findings indicated that indeed availability norms are significantly associated with more burnout symptoms and that both heightened telepressure and reduced autonomy mediated this relationship.

Discussion

This study contributes to theory and practice as we offer insight into how availability norms at work could be detrimental for the occupational health of employees, which can be taken into account when setting up rules and regulations at work.
Sharing of cultural values and heritage through storytelling in the digital age
Authors: Zort Çağın , Karabacak Esra , Öznur Şevket , Dağlı Gökmen
Published: February 2023

Introduction

Sharing cultural values in this digital age for young generations who are digital natives is highly important and, in this respect, the aims of this study are to evaluate experts’ opinions on sharing cultural values in this digital age based on their experience, the roles of educators and families with respect to the sharing of cultural values through storytelling in the digital age, and also capture how cultural values can be explained with metaphors.

Methods

A focus group interview was conducted with teachers and vice headmasters from public primary and secondary schools in the Northern part of Cyprus within the age range of 30-50 years that are considered to be experts based on their 10 years and above of teaching experience. Data were analyzed through line-by-line coding to create themes.

Results

Findings revealed that cultural values are eroding, and in sharing cultural values with storytelling in the digital age, the roles of educators and families are essential. Cultural values are the treasures and mirrors of society that should be preserved and transmitted to the younger generations and this can be accomplished through participation in digital platforms, and when such participatory cultural heritage projects are planned with a community-oriented background and human-centered computing concentration.

Discussion

This research sheds a light to indicate the importance of the storytelling approach for sharing cultural values and heritage. It is significant to address the merits of technology in transferring cultural values and heritage. In addition to this, this study is limited to one specific context that can be further explored as a cross-cultural analysis.
Reifying “river”: Unpacking pluriversal possibilities in rejuvenation surrounding the Adi Ganga of Kolkata
Authors: Bhattacharya Shreyashi , Mukherjee Jenia , Choudry Anuradha , Ghosh Raktima
Published: February 2023

Using the River Adi Ganga as the case study and implementing the historical urban political ecology (HUPE) framework, in this article we demonstrate multiple trends and trajectories that comprise city-river relationships. We explore coeval ontologies framing “river,” “riverine space” and “river rejuvenation” schemes, encapsulating “storylines” through the deployment of multi-modal (qualitative) research methodologies to trace and document plural perceptions on the river that was declared to be “dead”—the pillaged stretch that embodied the construction of the metro pillars. Media journalists and activists' narrative, that this act had slaughtered the river and robbed her of her original flows and services, has crafted a deep imprint on the citizens of Kolkata who consider the river as a stinking sink, offering minimal sewerage facilities to the city. Our paper challenges this linear depiction and weaves together positive moments, events and actions that keep the river flowing—shaping and in turn being shaped by (more-than)human actors across long temporal units and scales. We integrate the numerous agential and stakeholder voices attached to the river, shedding light on various (un)successful attempts to revive the river beyond global conceptualizations of what a “river” should be. We believe that the unfolding of this “pluriverse” will forge sustainable understandings of the river's current challenges and existing opportunities toward a collaborative blueprint through knowledge coproduction, stakeholder mobilization and actions. While our empirical frame of reference focuses on micro-realities surrounding a particular river on a specific urban hydroscape, our theoretical conceptualization framings and methodological applications will have potentials to be implemented at scales.
The need for adoption of neural HPC (NeuHPC) in space sciences
Authors: Karimabadi Homa , Wilkes Jason , Roberts D. Aaron
Published: February 2023

A major challenge facing scientists using conventional approaches for solving PDEs is the simulation of extreme multi-scale problems. While exascale computing will enable simulations of larger systems, the extreme multiscale nature of many problems requires new techniques. Deep learning techniques have disrupted several domains, such as computer vision, language (e.g., ChatGPT), and computational biology, leading to breakthrough advances. Similarly, the adaptation of these techniques for scientific computing has led to a new and rapidly advancing branch of High-Performance Computing (HPC), which we call neural-HPC (NeuHPC). Proof of concept studies in domains such as computational fluid dynamics and material science have demonstrated advantages in both efficiency and accuracy compared to conventional solvers. However, NeuHPC is yet to be embraced in plasma simulations. This is partly due to general lack of awareness of NeuHPC in the space physics community as well as the fact that most plasma physicists do not have training in artificial intelligence and cannot easily adapt these new techniques to their problems. As we explain below, there is a solution to this. We consider NeuHPC a critical paradigm for knowledge discovery in space sciences and urgently advocate for its adoption by both researchers as well as funding agencies. Here, we provide an overview of NeuHPC and specific ways that it can overcome existing computational challenges and propose a roadmap for future direction.
Diagnosis and surgical management strategy for pediatric small bowel obstruction: Experience from a single medical center
Authors: Liu Mingzhu , Cheng Fengchun , Liu Xijie , Zheng Bufeng , Wang Feifei , Qin Chengwei , Ding Guojian , Fu Tingliang , Geng Lei
Published: February 2023

Identifying Bowel strangulation and the approach and timing of surgical intervention for pediatric SBO are still uncertain. In this study, 75 consecutive pediatric patients with surgically confirmed SBO were retrospectively reviewed. The patients were divided into group 1 (n = 48) and group 2 (n = 27) according to the presence of reversible or irreversible bowel ischemia, which was analyzed based on the degree of ischemia at the time of operation. The results demonstrated that the proportion of patients with no prior abdominopelvic surgery was higher, the serum albumin level was lower, and the proportion of patients in which ascites were detected by ultrasonography was higher in group 2 than that in group 1. The serum albumin level was negatively correlated with ultrasonographic findings of the fluid sonolucent area in group 2. There were significant differences in the choice of surgical approach between group 1 and group 2. A symptom duration of >48 h was associated with an increased bowel resection rate. The mean length of hospital stay was shorter in group 1 than that in group 2. In conclusion, immediate surgical intervention should be considered in patients with a symptom duration of >48 h or the presence of free ascites between dilated small bowel loops on ultrasonography. Laparoscopic exploration is recommended as first-line treatment in patients with stable status.
Curiosity in zebrafish (Danio rerio)? Behavioral responses to 30 novel objects
Authors: Franks Becca , Gaffney Leigh P. , Graham Courtney , Weary Daniel M.
Published: February 2023

Curiosity—the motivation to seek out information—has been studied widely across the animal kingdom. To investigate curiosity in zebrafish we presented 30 novel objects to groups of zebrafish housed in semi-naturalistic tanks (6 tanks; 10 fish/tank; 10-min presentations). During the first 100 s and final 100 s of each object's 10-min presentation period, we recorded each group's: (i) latency to approach the object, (ii) attraction to the object, (iii) social dynamics: agonistic behavior and group cohesion and coordination, and (iv) diving behavior, a stress response in zebrafish. Comparing these behaviors to a 100 s baseline period when no object was present, we tested for neophobia (avoidance of novelty), neophilia (overall attraction to novelty), sustained interest (prolonged attraction to at least some presentations), discriminant interest (certain objects eliciting more attention than others), habituation (loss of interest over time), and alterations to social and stress behaviors. Zebrafish groups readily approached all objects (1 s median latency), were neophilic throughout all object presentations, and showed systematic sustained interest only for some object presentations at the beginning of the study (object presentations 1–10). Over the course of the study, zebrafish also showed signs of habituation such that by the final ten object presentations (21-30), there were no signs of overall sustained interest. During the beginning of the study (object presentations 1–10), we also found evidence for specific object-driven interest, with object ID accounting for 11% of the variability in interest scores (p < 0.01), and object-driven interest corresponding to alterations in social behavior: decreased aggression (p < 0.02), increased group cohesion (p < 0.02), and increased group coordination (p < 0.05). By explicitly investigating curiosity in fish, this work reveals that under certain conditions, zebrafish voluntarily engage in cognitive stimulation opportunities. More work is needed to clarify what types of information zebrafish find most rewarding and how long-term exposure to such opportunities may affect fish welfare.
Evaluations of the Disease Surveillance Centre network in Scotland: What parts has it reached?
Authors: Duncan Andrew J. , Eze Jude I. , Brülisauer Franz , Stirling Julie M. , Jennings Amy , Tongue Sue C.
Published: February 2023

Regular evaluation is a prerequisite for systems that provide surveillance of animal populations. Scotland's Rural College Veterinary Services' Disease Surveillance Centre (DSC) network plays an integral part in surveillance to detect new and re-emerging threats within animal populations, predominantly livestock. In response to surveillance reviews and proposed changes to the network, an initial evaluation of diagnostic submissions data in 2010 to mid-2012 established a baseline “footprint,” while highlighting challenges with the data. In this recent evaluation for the period 2013–2018, we developed a new denominator using a combination of agricultural census and movement data, to identify relevant holdings more accurately. Iterative discussions between those processing submissions data and those involved in collection at source took place to understand the intricacies of the data, establish the most appropriate dataset, and develop the processes required to optimise the data extraction and cleansing. The subsequent descriptive analysis identifies the number of diagnostic submissions, the number of unique holdings making submissions to the network and shows that both the surrounding geographic region of, and maximum distance to the closest DSC vary greatly between centres. Analysis of those submissions classed as farm animal post-mortems also highlights the effect of distance to the closest DSC. Whether specific differences between the time periods are due to changes in the behavior of the submitting holdings or the data extraction and cleaning processes was difficult to disentangle. However, with the improved techniques producing better data to work with, a new baseline footprint for the network has been created. This provides information that can help policy makers and surveillance providers make decisions about service provision and evaluate the impact of future changes. Additionally, the outputs of these analyses can provide feedback to those employed in the service, providing evidence of what they are achieving and why changes to data collection processes and ways of working are being made. In a different setting, other data will be available and different challenges may arise. However, the fundamental principles highlighted in these evaluations and the solutions developed should be of interest to any surveillance providers generating similar diagnostic data.
The Improvement and Application of the Electrogeochemical Exploration Method
Authors: Kang Ming , Guo Huanzhao , Zhu Wende , Luo Xianrong , Yang Jianwen
Published: February 2023

The anionic and cationic species of elements from deeply buried deposits migrate to the near surface driven by various geological forces. The applied electric field is used to collect these active ions to the designed electrode, and ions with a negative charge go to the anode and positive to the cathode, which is called electrogeochemical exploration method, also called CHIM. Prospecting effect has been investigated by researchers since the coming up of CHIM. However, the cumbersome technical equipment, complex techniques and low production efficiency have restricted its potential application in field geological survey. This paper presents the newly developed CHIM alternatives that are electrified by a low voltage dipole. The improved technique allows both anionic and cationic species to be extracted simultaneously in an anode and in a cathode. Compared with the conventional CHIM method, the innovative techniques called dipole electrogeochemical method are characterized by simple instrumentation, low cost and easy operation in field, and in particular enables simultaneous extraction of anionic and cationic species of elements, from which more information can be derived with higher extraction efficiency. The “dipole” electrogeochemical extraction method was proposed and applied in the experiments of the Yingezhuang gold ore in Zhaoyuan, Shandong Province, the 210 gold ore in Jinwozi, Xinjiang Province, and the Daiyinzhang gold polymetallic deposit in Wutaishan, Shanxi Province. There are clearly anomalies above the gold ore body, indicating the effectiveness and feasibility of the improved dipole electrogeochemical method in both scientific research and mineral exploration. The results of anode extraction in several mining areas have shown good results, indicating that gold may be mainly negatively charged. In fact, many metal nanoparticles, clay minerals, or complexes of metal ions are negatively charged, so they migrate to the anode electrode and enrich.
Repurposing Atovaquone as a Therapeutic against Acute Myeloid Leukemia (AML): Combination with Conventional Chemotherapy Is Feasible and Well Tolerated
Authors: Stevens Alexandra McLean , Schafer Eric S. , Li Minhua , Terrell Maci , Rashid Raushan , Paek Hana , Bernhardt Melanie B. , Weisnicht Allison , Smith Wesley T. , Keogh Noah J. , Alozie Michelle C. , Oviedo Hailey H. , Gonzalez Alan K. , Ilangovan Tamilini , Mangubat-Medina Alicia , Wang Haopei , Jo Eunji , Rabik Cara A. , Bocchini Claire , Hilsenbeck Susan , Ball Zachary T. , Cooper Todd M. , Redell Michele S.
Published: February 2023

Survival of pediatric AML remains poor despite maximized myelosuppressive therapy. The pneumocystis jiroveci pneumonia (PJP)-treating medication atovaquone (AQ) suppresses oxidative phosphorylation (OXPHOS) and reduces AML burden in patient-derived xenograft (PDX) mouse models, making it an ideal concomitant AML therapy. Poor palatability and limited product formulations have historically limited routine use of AQ in pediatric AML patients. Patients with de novo AML were enrolled at two hospitals. Daily AQ at established PJP dosing was combined with standard AML therapy, based on the Medical Research Council backbone. AQ compliance, adverse events (AEs), ease of administration score (scale: 1 (very difficult)-5 (very easy)) and blood/marrow pharmacokinetics (PK) were collected during Induction 1. Correlative studies assessed AQ-induced apoptosis and effects on OXPHOS. PDX models were treated with AQ. A total of 26 patients enrolled (ages 7.2 months–19.7 years, median 12 years); 24 were evaluable. A total of 14 (58%) and 19 (79%) evaluable patients achieved plasma concentrations above the known anti-leukemia concentration (>10 µM) by day 11 and at the end of Induction, respectively. Seven (29%) patients achieved adequate concentrations for PJP prophylaxis (>40 µM). Mean ease of administration score was 3.8. Correlative studies with AQ in patient samples demonstrated robust apoptosis, OXPHOS suppression, and prolonged survival in PDX models. Combining AQ with chemotherapy for AML appears feasible and safe in pediatric patients during Induction 1 and shows single-agent anti-leukemic effects in PDX models. AQ appears to be an ideal concomitant AML therapeutic but may require intra-patient dose adjustment to achieve concentrations sufficient for PJP prophylaxis.