bluuebunny/crossref_metadata_2025 huggingface dataset:
“After All, No One Is Superhuman Here”: An Analysis of the Perceived Effects of Managerial Athleticism
Authors: Raisio Harri , Kuorikoski Tero , Rantala Tero , Rask Mikko
Published: October 2021

Executive duties have long been described as becoming more intensive and demanding. One contributing factor is a perceived increase in the complexity of the operating environment. A high level of physical fitness has been suggested to support responses to those demands. This study aims to contribute to the topic by producing a holistic view informed by asking how physically active executive-level leaders perceive the impact (whether positive or negative) of managerial athleticism on the level of the individual, organization, and society. Twenty physically active high-level leaders were interviewed for the study. The sample was almost equally split by gender, represented a diverse range of societal sectors, and engaged in many different forms of exercise. The results support previous research; however, this study provides a more nuanced view of the topic. Although the interviewees saw many good aspects of physical exercise, such as providing physical, affective, cognitive, and social resources, they were also able to think critically about their personal relationship with exercising and their attitudes toward others taking physical exercise. The study highlights the role of balancing the harmonious and obsessive aspects of the relationship with exercising. Instead of a disproportionate study of extreme cases, we conclude that it would be worthwhile studying leaders with a harmonious relationship with exercise to ensure results do not become biased and physically active leaders are not stereotyped.
Exploring the Gender-Specific Adaptive Responses to Climate Variability: Application of Grazing Game in the Semi-Arid Region of Ghana
Authors: Mensah Michael , Villamor Grace B. , Fosu-Mensah Benedicta Y. , Vlek Paul L. G.
Published: October 2021

Regional climate change assessments show a likely temperature increase that is higher than the global average for all seasons in Africa, which would have extreme negative implications for ecosystem health and productivity. Most extreme climate change effects in West Africa are predicted to occur in desert and grassland areas. It is important for smallholder farmers in this region to understand the implications of these projections to their livelihood and to identify appropriate adaptation strategies. A grazing game was used to explore gender-specific adaptive responses to climate variability in the semiarid region of Ghana. The game was designed to understand the decision-making processes that result in the overgrazing of animals, leading to desertification based on the players’ interactions with the environment. A total of 44 grazing games comprising 22 games for male-headed households (HH) and 22 games for female-HH were played from August to December 2014 from 14 communities within the Bolgatanga Municipality and the Bongo district. The study revealed that males migrate to the southern part of the country to work on other people’s farms during the dry season as an adaptation strategy, while females engage in off-farm activities such as shea-butter production and basketry. Results of the game showed that males produced the highest number of cattle but created the largest desert patches. Females, on the other hand, were more conscious about the environment (long-term condition of the rangeland) than the short-term income benefits from the sale of cattle; hence, they created fewer desert patches. Strategies such as reducing the number of cattle to allow for the re-growth of vegetation in periods of feed scarcity, ploughing for one another using bulls, and family support using income from the sale of livestock were employed by both gender groups. The involvement of female farmers in decision-making is crucial to improve natural resource management.
Consumers’ Attitude towards Sustainable Food Consumption during the COVID-19 Pandemic in Romania
Authors: Muresan Iulia C. , Harun Rezhen , Arion Felix H. , Brata Anca Monica , Chereches Ioan Aurel , Chiciudean Gabriela O. , Dumitras Diana E. , Oroian Camelia F. , Tirpe Olivia Paula
Published: October 2021

The COVID-19 pandemic affected consumers’ behavior worldwide. This paper aims to analyze consumers’ sustainable food behavior during the COVID-19 pandemic. The research was based on an online survey during May–October 2020 among 859 Romanian consumers. Principal component analysis and cluster analysis were employed to group the consumers based on their behavior. Furthermore, the binary-logistic regression was used to identify the socio-demographic profile of the identified groups. Based on the cluster analysis, the consumers were grouped into three main groups: indifferent, pro-environment protection, and health concerned. The results indicate a positive attitude towards sustainable food behavior. Consumers’ attitude towards sustainable food choice is mainly influenced by age and education level. The study offers valuable information for future public policy and marketing campaign regarding sustainable food behavior.
Crop Establishment Methods and Weed Management Practices Affect Grain Yield and Weed Dynamics in Temperate Rice
Authors: Jehangir Intikhab Aalum , Hussain Ashaq , Sofi Najeeb. R. , Wani Shabir. H. , Ali Omar M. , Abdel Latef Arafat Abdel Hamed , Raja Waseem , Bhat M. Anwar
Published: October 2021

Higher demand and cost of labor and water shortage have forced the farmers to look for an alternate method of cultivation in rice as a substitute to the existing conventional transplanting. Dry direct seeding and water seeding have emerged as better alternatives over transplanting method. These methods not only result in labor saving, but also result in significant water saving in rice. These are important adaptation strategies to the impending climate change. However, the direct seeding method is confronted with severe weed infestation and yield losses if weeds are not managed well. Against this backdrop, a field study was undertaken during kharif seasons of 2019 and 2020 to evaluate the effect of crop establishment methods and weed management practices on rice and its associated weed flora. The results demonstrated that grain yields obtained under water seeding (WS) were statistically at par with transplanting (CT), but significantly superior to dry direct seeding (DDSR). Yield attributes were significantly superior in WS as compared DDSR, but were at par with CT. Weed density followed the order of DDSR > WS > CT. With the advancement in age of the crop, sedges dominated in DDSR, whereas broad-leafweeds (BLW) dominated in WS and CT methods of establishment. All the herbicides reduced the weed density significantly as compared to weedy check. Penoxulam (PE) reduced the weed density and weed dry matter on an average by 91% and 92% at 30 DAS/DAT over weedy check, respectively. PE proved significantly superior in controlling all the sedges and grasses but was less effective against BLW. Maximum reduction in yield due to weeds was observed in weedy check (WC) (58%) and the lowest was observed in PE (3%). Application of PE @ 22.5 g ha−1 under the WS method of crop establishment resulted in highest average weed control efficiency and grain yield.
Comparing Soil vs. Foliar Nitrogen Supply of the Whole Fertilizer Dose in Common Wheat
Authors: Ferrari Manuel , Dal Cortivo Cristian , Panozzo Anna , Barion Giuseppe , Visioli Giovanna , Giannelli Gianluigi , Vamerali Teofilo
Published: October 2021

Late-season N application through foliar spraying is recognized as an efficient agronomic practice for improving grain quality in common wheat, although the major part of N is still supplied by soil fertilization. This study assessed the impact of various N doses entirely applied by repeated foliar sprayings on wheat growth, yield and quality, in comparison with conventional soil fertilization management with a recommended dose of 160 kg N ha−1 as ammonium nitrate (C-M). Doses of 96, 104 and 120 kg N ha−1 as both UAN (urea-ammonium-nitrate) and urea applied by foliar spraying were evaluated in a 2-year field trial in Northern Italy in a silty loam soil with 1.7% organic matter. Here, it was demonstrated that the canopy greenness was similar in all treatments, with slight grain yield increases by the lowest foliar N dose vs. C-M. The higher N foliar doses mainly improved the grain protein content and both high- and low-molecular-weight glutenin subunits (HMW-GS, LMW-GS), particularly with urea. It is concluded that in our fertile soil, managing N fertilization exclusively through foliar spraying is feasible without compromising grain yield and ameliorating quality at the same time. Improved nutrient use efficiency and beneficial environmental effects are also expected by reducing the nitrogen load on the agricultural fields by 25–40%.
Contrary to Red, Blue Monochromatic Light Improves the Bioactive Compound Content in Broccoli Sprouts
Authors: Yang Li , Fanourakis Dimitrios , Tsaniklidis Georgios , Li Kun , Yang Qichang , Li Tao
Published: October 2021

Broccoli sprouts are rich in health-promoting bioactive compounds. Their content depends on both cultivation light quality and temperature. However, these effects have been previously addressed in isolation. Here, the dual inputs of cultivation light quality [blue (B), red (R), mixture of R and B (R+B), mixture of R and UVA (R+UVA)] and air temperature (15, 19, and 23 °C) on determining growth, external quality, and the cotyledon and hypocotyl content of five major bioactive compounds were investigated. The carbohydrate status at harvest and postharvest ratio of variable to maximum fluorescence (Fv/Fm) were also assessed. Hypocotyl length was generally enhanced under monochromatic light (R or B) and elevated temperature. Total phenolic, total flavonoid, and glucoraphanin contents were generally higher in cotyledon as compared to hypocotyl. Hypocotyl anthocyanin, total phenolic, total flavonoid, and ascorbic acid contents were generally enhanced by R+B, and were decreased by R. Cotyledon content in these metabolites was generally stimulated by B, and reduced under R or R+UVA. Temperature affected metabolite content depending on the metabolite, organ, and light quality. Lower temperatures, R (23 °C) or R+UVA (15, 19, and 23 °C) were associated with decreased postharvest Fv/Fm. In conclusion, low cultivation temperature (<23 °C), as well as R or R+UVA ought to be avoided. Instead, B and R+B are suitable, with B being preferable, owing to better external quality and enhanced metabolite content in cotyledon which generally holds higher content than hypocotyl.
Silicon Nanoparticles Mitigate Hypoxia-Induced Oxidative Damage by Improving Antioxidants Activities and Concentration of Osmolytes in Southern Highbush Blueberry Plants
Authors: Iqbal Zafar , Sarkhosh Ali , Balal Rashad Mukhtar , Rauf Saeed , Khan Naeem , Altaf Muhammad Ahsan , Camara-Zapata Jose M. , Garcia-Sanchez Francisco , Shahid Muhammad Adnan
Published: October 2021

Climate change exacerbates flooding problems due to hurricanes followed by heavy rains, particularly in sub-tropical regions. Consequently, submerged plants experience hypoxia stress which limits agronomic and horticultural crop growth and production. Hypoxia causes oxidative damage by accelerating the lipid peroxidation associated with O2- and H2O2 levels. Additionally, hypoxia increases the accumulation of organic osmoprotectants and antioxidant activity, whereas it decreases the macronutrient (N, P, K, and Zn) uptake. This study aimed at investigating the effects of flooding-induced hypoxia stress on the growth and the physiological, biochemical, and nutritional characteristics of the hydroponically grown southern highbush blueberry (cv. Jewel) plants. In addition, the hypoxia-mitigating effects of conventional silicon (Si-C) and silicon nanoparticles (SiNPs) and their application methods (foliar vs. foliar and rootzone application) were also appraised. Both the Si-C and the SiNPs efficiently alleviated hypoxia-induced oxidative and osmotic damage to cells by enhancing the activities of the enzymatic antioxidants (ascorbate peroxidase, catalase, dehydroascorbate reductase, superoxide dismutase, peroxidase, guaiacol peroxidase, monodehydroascorbate, reductase); the non-enzymatic antioxidants (ascorbic acid and glutathione contents); and the accumulation of compatible solutes (proline and glycinebetaine) in leaves and roots. However, the SiNPs were more effective than Si-C at improving antioxidant activities and osmolytes formation. A strong negative correlation between the antioxidant activities and the lipid peroxidation rate was observed in the SiNP-treated plants under hypoxia stress. The SiNPs also regulated nutrient uptake by increasing the K, N, P, and Zn concentrations while decreasing Fe and Mn concentrations to a less toxic level. Blueberry plants treated with SiNPs responded more effectively to hypoxia stress by maintaining higher antioxidant and osmoprotectant concentrations than blueberry plants treated with Si-C. Additionally, the foliar and rootzone applications yielded better results than the foliar applications only.
Blueberry Yield and Soil Mineral Nitrogen Response to Nitrogen Fertilizer and Nitrification Inhibitors under Drip-Fertigation Systems
Authors: Messiga Aimé J. , Nyamaizi Sylvia , Yu Shaobing , Dorais Martine
Published: October 2021

In blueberry plantings, nitrification can result in losses of mineral nitrogen (N) through leaching because blueberries prefer ammonium (NH4+) over nitrate (NO3−). The objective of this study was to assess the effects of two rates of N fertilizer, mixed or not with nitrification inhibitors (NI) and applied through two fertigation systems, on berry yield and the concentrations of NH4+-N and NO3−-N along the soil profile. Thus, nine combinations of treatments including two N fertilizer rates (60 and 120 kg N ha−1), two NI (with DCD + Nitra-pyrin or without) and two fertigation application methods (buried and suspended drip lines) and a control (0 kg N ha−1) were tested over three years (2016–2018) in a long-term blueberry planting. Berry yield was on average 47.32 Mg ha−1 in 2016 and 26.86 Mg ha−1 in 2018. In 2017, berry yield varied between 8.60 Mg ha−1 under the control and 11.66 Mg ha−1 with 120 kg N ha−1 applied through suspended drip lines. Low berry yield in 2017 was due to a heavy pruning to rejuvenate the plants. In 2016, the concentration of NH4+-N in the sawdust mulch layer varied between 13.1 and 27.1 mg kg−1 in the spring, 11.4 and 32.1 mg kg−1 in the summer, and 7.9 and 72.9 mg kg−1 in the fall; the concentration of high NH4+-N along the soil profile did not exceed 5 mg kg−1. High concentrations of NH4+-N in the sawdust mulch layer were associated with NI, but did not translate to high berry yields. The concentration of NO3−-N in the soil profile reached 42.6 mg kg−1 in the summer and 39.0 mg kg−1 in the fall and these high concentrations were associated with NI. In 2017 and 2018, there was no effect of NI on NH4+-N concentrations even in the layer of sawdust mulch which was not consistent with the results obtained in 2016. High concentrations of NO3−-N were measured beneath the sawdust mulch layer with treatments including N fertilizer alone or mixed with NI. It is possible that NH4+ retained in the layer of sawdust mulch and not taken up by plant roots was subsequently oxidized to NO3− at the end of the residence time of NI in the soil. Our results showed that high concentrations of NO3−-N along the soil profile occurred mainly during the summer which could be explained by irrigation water driving NO3− leaching.
Distress Analysis of Mice with Cervical Arteriovenous Fistulas
Authors: Xie Wentao , Palme Rupert , Schafmayer Clemens , Zechner Dietmar , Vollmar Brigitte , Grambow Eberhard
Published: October 2021

The welfare of laboratory animals is a consistent concern for researchers. Its evaluation not only fosters ethical responsibility and addresses legal requirements, but also provides a solid basis for a high quality of research. Recently, a new cervical arteriovenous model was created in mice to understand the pathophysiology of arteriovenous fistula, which is the most commonly used access for hemodialysis. This study evaluates the distress caused by this new animal model. Ten male C57B6/J mice with cervical arteriovenous fistula were observed for 21 days. Non-invasive parameters, such as body weight, faecal corticosterone metabolites, burrowing activity, nesting activity and distress scores were evaluated at each time point. Six out of ten created arteriovenous fistula matured within the observation time as defined by an increased diameter. The body weight of all animals was reduced after surgery but recovered within five days. In addition, the distress score was significantly increased during the early time point but not at the late time point after arteriovenous fistula creation. Neither burrowing activity nor nesting behaviour were significantly reduced after surgical intervention. Moreover, faecal corticosterone metabolite concentrations did not significantly increase. Therefore, the cervical murine arteriovenous fistula model induced moderate distress in mice and revealed an appropriate maturation rate of the fistulas.
ssGBLUP Method Improves the Accuracy of Breeding Value Prediction in Huacaya Alpaca
Authors: Mancisidor Betsy , Cruz Alan , Gutiérrez Gustavo , Burgos Alonso , Morón Jonathan Alejandro , Wurzinger Maria , Gutiérrez Juan Pablo
Published: October 2021

Improving textile characteristics is the main objective of alpaca breeding. A recently developed SNP chip for alpacas could potentially be used to implement genomic selection and accelerate genetic progress. Therefore, this study aimed to compare the increase in prediction accuracy of three important fiber traits: fiber diameter (FD), standard deviation of fiber diameter (SD), and percentage of medullation (PM) in Huacaya alpacas. The data contains a total pedigree of 12,431 animals, 24,169 records for FD and SD, and 8386 records for PM and 60,624 SNP markers for each of the 431 genotyped animals of the Pacomarca Genetic Center. Prediction accuracy of breeding values was compared between a classical BLUP and a single-step Genomic BLUP (ssGBLUP). Deregressed phenotypes were predicted. The accuracies of the genetic and genomic values were calculated using the correlation between the predicted breeding values and the deregressed values of 100 randomly selected animals from the genotyped ones. Fifty replicates were carried out. Accuracies with ssGBLUP improved by 2.623%, 6.442%, and 1.471% on average for FD, SD, and PM, respectively, compared to the BLUP method. The increase in accuracy was relevant, suggesting that adding genomic data could benefit alpaca breeding programs.
Investigating Genetic and Phenotypic Variability of Queen Bees: Morphological and Reproductive Traits
Authors: Facchini Elena , De Iorio Maria Grazia , Turri Federica , Pizzi Flavia , Laurino Daniela , Porporato Marco , Rizzi Rita , Pagnacco Giulio
Published: October 2021

The quality of the honeybee queen has an important effect on a colony’s development, productivity, and survival. Queen failure or loss is considered a leading cause for colonies’ mortality worldwide. The queen’s quality, resulting from her genetic background, developmental conditions, mating success, and environment, can be assessed by some morphological measures. The study aims to investigate variability for traits that could assess the quality of the queen. Related animals were enrolled in this study. Variance components were estimated fitting a mixed animal model to collected data. Heritabilities of body and tagmata weights ranged from 0.46 to 0.54, whereas lower estimates were found for the tagmata width and wing length. Heritabilities estimated for the spermatheca diameter and volume, number of ovarioles, and number of sperms were 0.17, 0.88, 0.70, and 0.57, respectively. Many phenotypic correlations related to size were high and positive, while weak correlations were found between morphology and reproductive traits. Introducing a queen’s traits in a selection program could improve colonies’ survivability. Further research should focus on better defining the correlations between the individual qualities of a queen and her colony’s performance.
Ecological Analysis of the Helminth Community of Microtus lusitanicus (Gerbe, 1879) (Rodentia) in Asturias (NW Spain)
Authors: Adalid Roser , Feliu Carles , Somoano Aitor , Miñarro Marcos , Ventura Jacint , Torres Jordi , Miquel Jordi , Fuentes Màrius Vicent
Published: October 2021

The Lusitanian pine vole, Microtus lusitanicus, an endemic fossorial rodent of the Iberian Peninsula, has a burrowing behaviour and prefers to live underground. It feeds on bark and roots causing severe damage to trees. In Asturias (NW Spain), where M. lusitanicus is considered a pest in several orchards, a faunistic-ecological study was carried out to describe the helminth community of this species and the main factors that could influence its helminth component species. For this purpose, our own collection of 710 voles from several orchards of various locations in Asturias was used. Eight helminth species, four cestodes and four nematodes, were found. Statistical non-parametric tests were used to analyse the effects of extrinsic and intrinsic factors on the diversity of the helminth community and species prevalence and abundance. The results show the influence of climate variables, the year and season of capture, as well as host age, on the diversity of the helminth community and the infection parameters of some helminth species, underlining the importance of their life cycles. In addition to shedding light on the helminth community of this rodent in Asturias, the results obtained could be used to improve the biological methods applied to fight the M. lusitanicus pest.
Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation
Authors: Wu Xiao , Zhao Huaxing , Lai Junkun , Zhang Ning , Shi Junsong , Zhou Rong , Su Qiaoyun , Zheng Enqin , Xu Zheng , Huang Sixiu , Hong Linjun , Gu Ting , Yang Jie , Yang Huaqiang , Cai Gengyuan , Wu Zhenfang , Li Zicong
Published: October 2021

Cloned animals generated by the somatic cell nuclear transfer (SCNT) approach are valuable for the farm animal industry and biomedical science. Nevertheless, the extremely low developmental efficiency of cloned embryos hinders the application of SCNT. Low developmental competence is related to the higher apoptosis level in cloned embryos than in fertilization-derived counterparts. Interleukin 17D (IL17D) expression is up-regulated during early mouse embryo development and is required for normal development of mouse embryos by inhibiting apoptosis. This study aimed to investigate whether IL17D plays roles in regulating pig SCNT embryo development. Supplementation of IL17D to culture medium improved the developmental competence and decreased the cell apoptosis level in cloned porcine embryos. The transcriptome data indicated that IL17D activated apoptosis-associated pathways and promoted global gene expression at embryonic genome activation (EGA) stage in treated pig SCNT embryos. Treating pig SCNT embryos with IL17D up-regulated expression of GADD45B, which is functional in inhibiting apoptosis and promoting EGA. Overexpression of GADD45B enhanced the developmental efficiency of cloned pig embryos. These results suggested that IL17D treatment enhanced the developmental ability of cloned pig embryos by suppressing apoptosis and promoting EGA, which was related to the up-regulation of GADD45B expression. This study demonstrated the roles of IL17D in early development of porcine SCNT embryos and provided a new approach to improve the developmental efficiency of cloned porcine embryos.
Implications of Nutritional Management on Fatty Acid Profiles of Southern White Rhinoceroses (Ceratotherium simum simum) Housed at Two Zoological Institutions
Authors: Wood Jordan , Jb Minter Larry , Tollefson Troy Neil , Bissell Heidi , Bibus Doug , Ange-van Heugten Kimberly
Published: October 2021

Southern white rhinoceroses (Ceratotherium simum simum) are African megaherbivores that are considered near threatened by the International Union for the Conservation of Nature. The fatty acid circulating values of these animals have not been thoroughly investigated. Fatty acids are critical for immune, heart, skin, and reproductive health, and may have a significant impact on the management and conservation of this species. Published data on fatty acids in this species is limited to incomplete profiles with very few animals in managed environments. The objectives of this research were to provide novel fatty acid percentage profiles for managed healthy southern white rhinoceroses, as well as to provide comparisons between two zoological institutions with differences in diet and climate during two distinct pasture growth periods. Whole blood samples were collected as dried blood spots from six rhinoceroses at the North Carolina Zoo (NC Zoo) and five rhinoceroses at Busch Gardens Tampa (BGT) in the low growth period (February to April) of 2019 and during the high growth period (July to September) of 2020. Fatty acid results indicated numerous differences when comparing the institutions within the same growth period and when comparing the same institution between its two growth periods. Most noteworthy were the higher levels of α-linolenic acid (18:3w3) and total omega-3 fatty acids and the lower linoleic acid (18:2w6), total omega-6 fatty acids, and omega-6 to omega-3 ratio found in the BGT population in both growth periods. This study provides novel percentages of fatty acids in managed southern white rhinoceroses and data on how fatty acid profiles may be altered between two housing locations via dietary differences in hay type and quantity, pasture availability via season, and pellet inclusion levels.
Does Systemic Methotrexate Therapy Induce Azole Resistance among Endogenous Candida Strains?
Authors: Żyrek Dawid , Nowicka Joanna , Pajączkowska Magdalena , Morgiel Ewa
Published: October 2021

Background: Research confirms that Candida spp. incubated with methotrexate develop multi-drug resistance to azoles, but it is not clear whether this phenomenon occurs in vivo in patients treated with cytostatics. The aim of the study was to assess whether systemic methotrexate therapy induces resistance to azoles among endogenous Candida strains in patients with rheumatological diseases. Methods: The test group consisted of 52 rheumatological patients on methotrexate therapy, who have never been exposed to fluconazole. The control group was composed of 49 individuals who have never been exposed to either methotrexate or fluconazole. Oral swab and clinical information were obtained from each participant. The acquired material was cultured, then each strain was isolated and identified (MALDI TOF). Subsequently, minimal inhibitory concentration (MIC) for fluconazole was determined. Results: MIC values ranged from <0.125 to 64 µg/mL with the most common result <0.125 µg/mL. Samples obtained from 4 patients of the test group and 2 patients of the control group contained strains resistant to fluconazole. Conclusions: Despite slightly higher incidence of fluconazole-resistant strains among patients on systemic methotrexate therapy, we found no solid evidence to support the hypothesis that methotrexate induces resistance to azoles among endogenous Candida strains in patients with rheumatological diseases.
Amyloid-β 25-35 Induces Neurotoxicity through the Up-Regulation of Astrocytic System Xc−
Authors: D’Ezio Veronica , Colasanti Marco , Persichini Tiziana
Published: October 2021

Amyloid-β (Aβ) deposition, a hallmark of Alzheimer’s disease, is known to induce free radical production and oxidative stress, leading to neuronal damage. During oxidative stress, several cell types (including astrocytes) can activate the nuclear factor erythroid 2-related factor 2 (Nrf2), a regulator of several phase II detoxifying and antioxidant genes, such as the System Xc− subunit xCT. Here, we studied (i) the effect of the Aβ fragment 25-35 (Aβ25-35) on Nrf2-dependent System Xc− expression in U373 human astroglial cells and (ii) the effect of Aβ25-35-induced astrocytic response on neuronal cell viability using an in vitro co-culture system. We found that Aβ25-35 was able to activate an antioxidant response in astrocytes, by inducing both Nrf2 activation and System Xc− up-regulation. However, this astrocytic response caused an enhanced cell mortality of co-cultured SH-SY5Y cells, taken as a neuronal model. Consistently, the specific System Xc− inhibitor sulfasalazine prevented the increase of both neuronal mortality and extracellular glutamate levels, thus indicating that the neurotoxic effect was due to an augmented release of glutamate through the transporter. The involvement of NMDA receptor activation in this pathway was also demonstrated using the specific inhibitor MK801 that completely restored neuronal viability at the control levels. The present study sheds light on the Nrf2/system Xc− pathway in the toxicity induced by Aβ25-35 and may help to better understand the involvement of astrocytes in neuronal death during Alzheimer’s disease.
Animal Models of Coenzyme Q Deficiency: Mechanistic and Translational Learnings
Authors: González-García Pilar , Barriocanal-Casado Eliana , Díaz-Casado María Elena , López-Herrador Sergio , Hidalgo-Gutiérrez Agustín , López Luis C.
Published: October 2021

Coenzyme Q (CoQ) is a vital lipophilic molecule that is endogenously synthesized in the mitochondria of each cell. The CoQ biosynthetic pathway is complex and not completely characterized, and it involves at least thirteen catalytic and regulatory proteins. Once it is synthesized, CoQ exerts a wide variety of mitochondrial and extramitochondrial functions thank to its redox capacity and its lipophilicity. Thus, low levels of CoQ cause diseases with heterogeneous clinical symptoms, which are not always understood. The decreased levels of CoQ may be primary caused by defects in the CoQ biosynthetic pathway or secondarily associated with other diseases. In both cases, the pathomechanisms are related to the CoQ functions, although further experimental evidence is required to establish this association. The conventional treatment for CoQ deficiencies is the high doses of oral CoQ10 supplementation, but this therapy is not effective for some specific clinical presentations, especially in those involving the nervous system. To better understand the CoQ biosynthetic pathway, the biological functions linked to CoQ and the pathomechanisms of CoQ deficiencies, and to improve the therapeutic outcomes of this syndrome, a variety of animal models have been generated and characterized in the last decade. In this review, we show all the animal models available, remarking on the most important outcomes that each model has provided. Finally, we also comment some gaps and future research directions related to CoQ metabolism and how the current and novel animal models may help in the development of future research studies.
Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis
Authors: Cao Mi-Xia , Xie Xiao-Dong , Wang Xin-Rui , Hu Wen-Yue , Zhao Yi , Chen Qi , Ji Lu , Wei Ying-Yi , Yu Mei-Ling , Hu Ting-Jun
Published: October 2021

To investigate the structure of Arthrospira platensis polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 106 Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.
Study of Mesh Pattern for Optically Transparent Flexible Antenna with Feedline
Authors: Yu Seulgi , Lee Soyeong , Lee Hoosung , Park Yong Bae
Published: October 2021

This paper presents a systematic parameter study on mesh pattern for optically transparent flexible antenna with feedline. In implementing a transparent flexible antenna using a metal mesh, transparency and performance of antenna and feedline are opposite factors. To understand how both elements are affected by the design parameters of the mesh, we analyze the performance of the feedlines and antennas according to the design parameters of diamond and square meshes. Moreover, the effect of the difference in the shape of diamond mesh and square mesh on performance is analyzed. The measured results of the fabricated samples offer the feasibility of implementing transparent feedlines and antennas with similar performance to nontransparent feedlines and antennas.
Cardiac and Cerebellar Histomorphology and Inositol 1,4,5-Trisphosphate (IP3R) Perturbations in Adult Xenopus laevis Following Atrazine Exposure
Authors: Asouzu Johnson Jaclyn , Nkomozepi Pilani , Opute Prosper , Mbajiorgu Ejikeme Felix
Published: October 2021

Despite several reports on the endocrine-disrupting ability of atrazine in amphibian models, few studies have investigated atrazine toxicity in the heart and cerebellum. This study investigated the effect of atrazine on the unique Ca2+ channel-dependent receptor (Inositol 1,4,5-trisphosphate; IP3R) in the heart and the cerebellum of adult male Xenopus laevis and documented the associated histomorphology changes implicated in cardiac and cerebellar function. Sixty adult male African clawed frogs (Xenopus laevis) were exposed to atrazine (0 µg/L (control), 0.01 µg/L, 200 µg/L, and 500 µg/L) for 90 days. Thereafter, heart and cerebellar sections were processed with routine histological stains (heart) or Cresyl violet (brain), and IP3R histochemical localization was carried out on both organs. The histomorphology measurements revealed a significant decrease in the mean percentage area fraction of atrial (0.01 µg/L and 200 µg/L) and ventricular myocytes (200 µg/L) with an increased area fraction of interstitial space, while a significant decrease in Purkinje cells was observed in all atrazine groups (p < 0.008, 0.001, and 0.0001). Cardiac IP3R was successfully localized, and its mean expression was significantly increased (atrium) or decreased (cerebellum) in all atrazine-exposed groups, suggesting that atrazine may adversely impair cerebellar plasticity and optimal functioning of the heart due to possible disturbances of calcium release, and may also induce several associated cardiac and neural pathophysiologies in all atrazine concentrations, especially at 500 µg/L.
Self-Organizing Maps to Assess the Recycling of Waste in Ceramic Construction Materials
Authors: Salas Ivan , Cifrian Eva , Andres Ana , Viguri Javier R.
Published: October 2021

Circular economy promotes the use of waste materials into new production processes as a key factor for resource efficiency. The construction sector, and specifically the fired clay industry, is able to assimilate large amounts of waste in their processes, without significantly altering the technical properties of products. The introduction of different waste in ceramic products at the laboratory level has been extensively studied in the literature, but most of these studies have not yet been scaled-up to industrial production. Differences in processing with respect to laboratory conditions introduces uncertainty in relation to the expected properties of the final products. This paper uses a Self-Organizing Map (SOM)-based methodology for analysing and assessing the incorporation of industrial waste, Waelz slag (WS) and foundry sand dust (FSD), in ceramic products obtained sequentially at laboratory, semi-industrial and industrial level, over technological properties and metals release. As a result, from the SOM analysis, a clustered map of the samples developed is obtained that highlights the most important parameters affecting the technological and environmental properties to be the type of clay and therefore, the firing temperature; secondly, the Waelz slag content, being independent of the foundry sand addition; and finally, the type and level of processing (laboratory-pressing, semi-industrial extruder, industrial extruder).
Crustal Electrical Structure of the Zhaheba Complex Imaged by Magnetotelluric Data and Its Tectonic Implications
Authors: Zhang Pingchuan , Yu Changqing , Zeng Xiangzhi
Published: October 2021

A Magnetotelluric profile stretching northward from the Wulungu Depression (on the northern margin of the Junggar Basin) to the Dulate arc (crossing the Zhaheba–Aermantai ophiolite belt) was carried out in an attempt to probe the crustal structure and properties of the East Junggar, NW China. Along the profile, the inversion model was used to determine the electrical structure of the crust and uppermost mantle. The results revealed that the crust of the eastern Junggar Basin is composed of the shallow low resistivity layer and underlying high resistivity bodies. There is a crustal detachment in the basement: the upper layer is a Hercynian folded basement and the lower is a Precambrian basement. The Zhaheba complex is characterized by relatively high resistivity, with a thickness of ~5 km, the bottom controlled by the Zhaheba–Aermantai fault. The crust of the Yemaquan arc is composed of the residual continental crust, characterized by stable resistance. The exposed intrusive rocks are characterized by irregular resistors. The crust of the Dulate arc is characterized by relatively low resistivity. The shallow low resistivity layers represent the Zhaheba depression composed of the Devonian-Permian volcanic and sedimentary rocks. The crustal conductive anomalies are related to the magmatism and mechanism of metal deposits in the post-collision period.
Embryotoxicity of Selective Serotonin Reuptake Inhibitors—Comparative Sensitivity of Zebrafish (Danio rerio) and African Clawed Frog (Xenopus laevis) Embryos
Authors: Blahova Jana , Doubkova Veronika , Plhalova Lucie , Lakdawala Pavla , Medkova Denisa , Vecerek Vladimir , Svobodova Zdenka , Faggio Caterina
Published: October 2021

Over the past twenty years, the prescription of antidepressant drugs has increased all over the world. After their application, antidepressants, like other pharmaceuticals, are excreted and enter the aquatic environment. They are dispersed among surface waters mainly through waste water sources, typically at very low concentrations— from a tenth up to hundreds of ng/L. Frequently detected antidepressants include fluoxetine and citalopram—both selective serotonin reuptake inhibitors. The aim of our study was to assess the embryotoxicity of fluoxetine hydrochloride and citalopram hydrochloride on the early life stages of zebrafish (Danio rerio) and the African clawed frog (Xenopus laevis). The embryos were exposed to various concentrations of the individual antidepressants and of their mixtures for 96 h. The tested levels included both environmentally relevant and higher concentrations for the evaluation of dose-dependent effects. Our study demonstrated that even environmentally relevant concentrations of these psychiatric drugs influenced zebrafish embryos, which was proven by a significant increase (p < 0.01) in the embryos’ heart rates after fluoxetine hydrochloride exposure and in their hatching rate after exposure to a combination of both antidepressants, and thus revealed a potential risk to aquatic life. Despite these results, we can conclude that the African clawed frog is more sensitive, since exposure to the highest concentrations of fluoxetine hydrochloride (10,000 μg/L) and citalopram hydrochloride (100,000 μg/L) resulted in total mortality of the frog embryos.
Diagnostic Value of Artificial Intelligence—Based Software in Detection of Large Vessel Occlusion in Acute Ischemic Stroke
Authors: Sawicki Marcin , Safranow Krzysztof , Wiska Lidia , Pasek Igor , Gajdziel Aleksandra , Gruszewski Michał , Poncyljusz Wojciech
Published: October 2021

Background: Aim of the study was to test the accuracy of AI-based software for detection of large vessel occlusion (LVO) with computed tomography angiography (CTA) in stroke patients using an experienced neuroradiologist’s evaluation as the reference. Methods: Consecutive patients who underwent multimodal brain CT for suspected acute ischemic stroke were retrospectively identified. The presence and site (classified as proximal and distal) of LVO were assessed in CTA by an experienced neuroradiologist as a reference and compared to readings of three medical students and AI-based software, the e-CTA. Results: One-hundred-eight participants with a mean age of 70 years (±12.6); 55 (50.9%) females were included. Neuroradiologist found LVO in 70 (64.8%) cases: 45 (41.7%) proximal, and 25 (23.1%) distal. The overall sensitivity for e-CTA was 0.67 (95%CI 0.55–0.78); 0.84 (95%CI 0.71–0.94) for proximal, and 0.36 (95%CI 0.18–0.57) for distal LVOs. Overall specificity and accuracy for e-CTA were 0.95 (95%CI 0.82–0.99) and 0.77 (95%CI 0.68–0.84), respectively. The student’s performance was similar to e-CTA. Conclusions: The tested software’s performance is acceptable for the detection of proximal LVOs, while it appears to be not accurate enough for distal LVOs.
The Effects of Polyphenol-Rich Black Elderberry on Oxidative Stress and Hepatic Cholesterol Metabolism
Authors: Jeon Sohyeon , Lee Sanggil , Choi Yeoni , Kim Bohkyung
Published: October 2021

Black elderberry (Sambucus Nigar) with high polyphenol content has been reported to have a hypolipidemic effect, but its underlying mechanisms have not been well characterized. In the present study, we investigated the effects of polyphenol-rich black elderberry (BEE) on oxidative stress and hepatic lipid metabolism. The total antioxidant activity of BEE was evaluated. The expression of genes for lipid metabolism was measured in 50 or 100 μg/mL of BEE-treated HepG2 cells. The mRNA and protein levels of genes for cholesterol metabolisms, i.e., sterol regulatory element-binding protein 2, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and low-density lipoprotein receptor, were decreased by BEE. There was marked induction of genes for high-density lipoprotein metabolism, i.e., scavenger receptor class B type 1and ATP-binding cassette (ABC) transporter A1 in BEE-treated cells. The expression of canalicular efflux transporter for hepatic cholesterol and bile acids, such as ABCG5/G8 and ABCB11, was significantly increased by BEE treatment. There was no alteration of the lipogenic genes, whereas BEE significantly decreased the expression of genes for fatty acid oxidation. BEE significantly altered the expression of histone deacetylase and sirtuins. These data suggest that the hypocholesterolemic effects of BEE may be attributed to the alteration of genes for hepatic cholesterol synthesis and flux.
A CNN-Based Wearable Assistive System for Visually Impaired People Walking Outdoors
Authors: Hsieh I-Hsuan , Cheng Hsiao-Chu , Ke Hao-Hsiang , Chen Hsiang-Chieh , Wang Wen-June
Published: October 2021

In this study, we propose an assistive system for helping visually impaired people walk outdoors. This assistive system contains an embedded system—Jetson AGX Xavier (manufacture by Nvidia in Santa Clara, CA, USA) and a binocular depth camera—ZED 2 (manufacture by Stereolabs in San Francisco, CA, USA). Based on the CNN neural network FAST-SCNN and the depth map obtained by the ZED 2, the image of the environment in front of the visually impaired user is split into seven equal divisions. A walkability confidence value for each division is computed, and a voice prompt is played to guide the user toward the most appropriate direction such that the visually impaired user can navigate a safe path on the sidewalk, avoid any obstacles, or walk on the crosswalk safely. Furthermore, the obstacle in front of the user is identified by the network YOLOv5s proposed by Jocher, G. et al. Finally, we provided the proposed assistive system to a visually impaired person and experimented around an MRT station in Taiwan. The visually impaired person indicated that the proposed system indeed helped him feel safer when walking outdoors. The experiment also verified that the system could effectively guide the visually impaired person walking safely on the sidewalk and crosswalks.
Fragility Curves for Material Characteristics and Damage Index for Brick Masonry Exposed to Freeze–Thaw Action
Authors: Uranjek Mojmir , Skrinar Matjaž , Imamović Denis , Peruš Iztok
Published: October 2021

In regions exposed to floods followed by cold weather, brick masonry as a structural basis of the building envelope can be damaged due to the accompanying phenomenon of freezing and thawing. The main purpose of the article is the development of a mathematical model able to predict the chosen mechanical parameters and damage index of brick wallets for a given number of freeze-thaw cycles. For this, a statistical model derived from experimental data is used. As a result, regression curves for Young’s modulus and ductility for two types of mortar are obtained. Furthermore, fragility curves for ductility and also the damage index, which is based on displacement ductility, are presented. The obtained results enable probabilistic risk assessments in the case of deteriorated ductility and increased damage on brick masonry due to freeze–thaw cycles.
Special Issue on Basics and Applications in Quantum Optics
Authors: Allevi Alessia , Olivares Stefano , Bondani Maria
Published: October 2021

Quantum technologies are advancing very rapidly and have the potential to innovate communication and computing far beyond current possibilities [...]
Sustainable Urban Water Management in China: A Case Study from Guangzhou and Kunming
Authors: Yang Shengnan , Huang Yiying , Radhakrishnan Mohanasundar , Rene Eldon R.
Published: October 2021

In China, the notion of a water sensitive city has gained popularity in urban water management as a result of the detrimental effects of flooding and pollution caused by developmental activities. Urban systems and their interrelationships are critical for long-term urban water management and water sensitivity. This article is a case study considering how a strength, weakness, opportunities, and threat (SWOT) analysis-based approach to urban water management interventions in Guangzhou and Kunming cities (China) enables decision makers to identify solutions for cities to become more water-sensitive and resilient. The similar difficulties and rewards with respect to the contexts of both cities were synthesized using SWOT analysis. The contextual SWOT analysis, in conjunction with the comprehensive inclusion of Sustainable Development Goals (SDGs) in intervention planning in these cities, revealed that a water-sensitive-cities approach requires the establishment of a comprehensively multi-objective rainwater management system; this approach would have the goals of reducing rainwater draining sources, controlling processes and adaptive measures, and governing the system to make it more resilient. The water strategy should be holistic and adaptive, capable of providing a broad range of ecological services and other social benefits consistent with the fulfilment of the Sustainable Development Goals, and adaptable to other Chinese cities seeking to achieve water sensitivity.