bluuebunny/crossref_metadata_2025 huggingface dataset:
Communication Skills and Transformational Leadership Style of First-Line Nurse Managers in Relation to Job Satisfaction of Nurses and Moderators of This Relationship
Authors: Jankelová Nadežda , Joniaková Zuzana
Published: March 2021

The job satisfaction of nurses is reflected in almost all organizational outputs of medical facilities. First-line nurse managers (FLNMs), who are directly related to subordinate nurses, have a great influence on this satisfaction. The aim of our paper is to examine the connection between communication skills and the transformation style of FLNMs management with the job satisfaction of nurses and to verify the influence of three moderators on the strength of this relationship. The chosen moderators—the practice of managing FLNMs, the degree of control (span of control) and psychosocial work—follow from theoretical studies. The moderating effect of the variable management practice is also significant from the point of view of Slovak legislation. The sample consisted of 132 FLNMs from five university hospitals in Slovakia. Data collection took place in the form of a questionnaire. All data were processed using the SPSS 24 software package. A series of regression analyzes were used to identify the proposed hypotheses. ANOVA analysis was used to analyze multiple dependencies. We worked at a 5% level of significance. The findings point to the strong direct effects of communication skills and the transformational leadership style of FLNMs on nurses’ job satisfaction. Moderation effects are mild, but significant in the case of management and span of control practices. The lower values of both variables reinforce the positive relationships among the two predictors and the job satisfaction of nurses. The third moderator, psychosocial work factors, also have a significant moderating effect, which is negative, and the higher value of this moderator mitigates both positive direct effects.
Liver Transplantation for Colorectal Liver Metastases: Current Management and Future Perspectives
Authors: Puia-Negulescu Serban , Lebossé Fanny , Mabrut Jean-Yves , Muller Xavier , Rossignol Guillaume , Antonini Teresa , Erard Domitille , Radenne Sylvie , Guillet Marielle , Souquet Jean-Christophe , Mohkam Kayvan , Lesurtel Mickael
Published: March 2021

Patients with nonresectable liver metastases from colorectal cancer have few therapeutic options and a dismal prognosis. Although liver transplantation for this indication has historically a poor reputation, recent advances in the field of chemotherapy and immunosuppression have paved the way to revisit the concept. New data have shown promising results that need to be validated in several ongoing clinical trials. Since liver grafts represent a scarce resource, several new tools are being explored to expand the donor pool for this indication. The purpose of this review is to present all current available data and perspectives about liver transplantation for nonresectable liver metastases from colorectal cancer.
Transcriptional Changes in Potato Sprouts upon Interaction with Rhizoctonia solani Indicate Pathogen-Induced Interference in the Defence Pathways of Potato
Authors: Zrenner Rita , Verwaaijen Bart , Genzel Franziska , Flemer Burkhardt , Grosch Rita
Published: March 2021

Rhizoctonia solani is the causer of black scurf disease on potatoes and is responsible for high economical losses in global agriculture. In order to increase the limited knowledge of the plants’ molecular response to this pathogen, we inoculated potatoes with R. solani AG3-PT isolate Ben3 and carried out RNA sequencing with total RNA extracted from potato sprouts at three and eight days post inoculation (dpi). In this dual RNA-sequencing experiment, the necrotrophic lifestyle of R. solani AG3-PT during early phases of interaction with its host has already been characterised. Here the potato plants’ comprehensive transcriptional response to inoculation with R. solani AG3 was evaluated for the first time based on significantly different expressed plant genes extracted with DESeq analysis. Overall, 1640 genes were differentially expressed, comparing control (−Rs) and with R. solani AG3-PT isolate Ben3 inoculated plants (+Rs). Genes involved in the production of anti-fungal proteins and secondary metabolites with antifungal properties were significantly up regulated upon inoculation with R. solani. Gene ontology (GO) terms involved in the regulation of hormone levels (i.e., ethylene (ET) and jasmonic acid (JA) at 3 dpi and salicylic acid (SA) and JA response pathways at 8 dpi) were significantly enriched. Contrastingly, the GO term “response to abiotic stimulus” was down regulated at both time points analysed. These results may support future breeding efforts toward the development of cultivars with higher resistance level to black scurf disease or the development of new control strategies.
Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals
Authors: Samiec Marcin , Skrzyszowska Maria
Published: March 2021

The effectiveness of somatic cell nuclear transfer (SCNT) in mammals seems to be still characterized by the disappointingly low rates of cloned embryos, fetuses, and progeny generated. These rates are measured in relation to the numbers of nuclear-transferred oocytes and can vary depending on the technique applied to the reconstruction of enucleated oocytes. The SCNT efficiency is also largely affected by the capability of donor nuclei to be epigenetically reprogrammed in a cytoplasm of reconstructed oocytes. The epigenetic reprogrammability of donor nuclei in SCNT-derived embryos appears to be biased, to a great extent, by the extranuclear (cytoplasmic) inheritance of mitochondrial DNA (mtDNA) fractions originating from donor cells. A high frequency of mtDNA heteroplasmy occurrence can lead to disturbances in the intergenomic crosstalk between mitochondrial and nuclear compartments during the early embryogenesis of SCNT-derived embryos. These disturbances can give rise to incorrect and incomplete epigenetic reprogramming of donor nuclei in mammalian cloned embryos. The dwindling reprogrammability of donor nuclei in the blastomeres of SCNT-derived embryos can also be impacted by impaired epigenetic rearrangements within terminal ends of donor cell-descended chromosomes (i.e., telomeres). Therefore, dysfunctions in epigenetic reprogramming of donor nuclei can contribute to the enhanced attrition of telomeres. This accelerates the processes of epigenomic aging and replicative senescence in the cells forming various tissues and organs of cloned fetuses and progeny. For all the above-mentioned reasons, the current paper aims to overview the state of the art in not only molecular mechanisms underlying intergenomic communication between nuclear and mtDNA molecules in cloned embryos but also intrinsic determinants affecting unfaithful epigenetic reprogrammability of telomeres. The latter is related to their abrasion within somatic cell-inherited chromosomes.
LATS1 Is a Mediator of Melanogenesis in Response to Oxidative Stress and Regulator of Melanoma Growth
Authors: Kazimierczak Urszula , Dondajewska Ewelina , Zajaczkowska Maria , Karwacka Marianna , Kolenda Tomasz , Mackiewicz Andrzej
Published: March 2021

The LATS1 kinase has been described as a tumor suppressor in various cancers. However, its role in melanoma has not been fully elucidated. There are several processes involved in tumorigenesis, including melanin production. Melanin content positively correlates with the level of reactive oxygen species (ROS) inside the cell. Accordingly, the purpose of the study was to assess the role of LATS1 in melanogenesis and oxidative stress and its influence on tumor growth. We have knocked down LATS1 in primary melanocytes and melanoma cells and found that its expression is crucial for melanin synthesis, ROS production, and oxidative stress response. We showed that LATS1 ablation significantly decreased the melanogenesis markers’ expression and melanin synthesis in melanocyte and melanoma cell lines. Moreover, silencing LATS1 resulted in enhanced oxidative stress. Reduced melanin content in LATS1 knocked down tumors was associated with increased tumor growth, pointing to melanin’s protective role in this process. The study demonstrated that LATS1 is highly engaged in melanogenesis and oxidative stress control and affects melanoma growth. Our results may find the implications in the diagnosis and treatment of pigmentation disorders, including melanoma.
Aerobic Exercise Ameliorates Cancer Cachexia-Induced Muscle Wasting through Adiponectin Signaling
Authors: Morinaga Makoto , Sako Naoki , Isobe Mari , Lee-Hotta Sachiko , Sugiura Hideshi , Kametaka Satoshi
Published: March 2021

Cachexia is a multifactorial syndrome characterized by muscle loss that cannot be reversed by conventional nutritional support. To uncover the molecular basis underlying the onset of cancer cachectic muscle wasting and establish an effective intervention against muscle loss, we used a cancer cachectic mouse model and examined the effects of aerobic exercise. Aerobic exercise successfully suppressed muscle atrophy and activated adiponectin signaling. Next, a cellular model for cancer cachectic muscle atrophy using C2C12 myotubes was prepared by treating myotubes with a conditioned medium from a culture of colon-26 cancer cells. Treatment of the atrophic myotubes with recombinant adiponectin was protective against the thinning of cells through the increased production of p-mTOR and suppression of LC3-II. Altogether, these findings suggest that the activation of adiponectin signaling could be part of the molecular mechanisms by which aerobic exercise ameliorates cancer cachexia-induced muscle wasting.
New Approach to Antifungal Activity of Fluconazole Incorporated into the Porous 6-Anhydro-α-l-Galacto-β-d-Galactan Structures Modified with Nanohydroxyapatite for Chronic-Wound Treatments—In Vitro Evaluation
Authors: Rewak-Soroczynska Justyna , Sobierajska Paulina , Targonska Sara , Piecuch Agata , Grosman Lukasz , Rachuna Jaroslaw , Wasik Slawomir , Arabski Michal , Ogorek Rafal , Wiglusz Rafal J.
Published: March 2021

New fluconazole-loaded, 6-Anhydro-α-l-Galacto-β-d-Galactan hydrogels incorporated with nanohydroxyapatite were prepared and their physicochemical features (XRD, X-ray Diffraction; SEM-EDS, Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy; ATR-FTIR, Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy), fluconazole release profiles and enzymatic degradation were determined. Antifungal activity of pure fluconazole was tested using Candida species (C. albicans, C. tropicalis, C. glabarata), Cryptococcus species (C. neoformans, C. gatti) and Rhodotorula species (R. mucilaginosa, R. rubra) reference strains and clinical isolates. Standard microdilution method was applied, and fluconazole concentrations of 2–250 µg/mL were tested. Moreover, biofilm production ability of tested isolates was tested on the polystyrene surface at 28 and 37 ± 0.5 °C and measured after crystal violet staining. Strains with the highest biofilm production ability were chosen for further analysis. Confocal microscopy photographs were taken after live/dead staining of fungal suspensions incubated with tested hydrogels (with and without fluconazole). Performed analyses confirmed that polymeric hydrogels are excellent drug carriers and, when fluconazole-loaded, they may be applied as the prevention of chronic wounds fungal infection.
Cross-Talk among Polymorphonuclear Neutrophils, Immune, and Non-Immune Cells via Released Cytokines, Granule Proteins, Microvesicles, and Neutrophil Extracellular Trap Formation: A Novel Concept of Biology and Pathobiology for Neutrophils
Authors: Tsai Chang-Youh , Hsieh Song-Chou , Liu Chih-Wei , Lu Cheng-Shiun , Wu Cheng-Han , Liao Hsien-Tzung , Chen Ming-Han , Li Ko-Jen , Shen Chieh-Yu , Kuo Yu-Min , Yu Chia-Li
Published: March 2021

Polymorphonuclear neutrophils (PMNs) are traditionally regarded as professional phagocytic and acute inflammatory cells that engulf the microbial pathogens. However, accumulating data have suggested that PMNs are multi-potential cells exhibiting many important biological functions in addition to phagocytosis. These newly found novel activities of PMN include production of different kinds of cytokines/chemokines/growth factors, release of neutrophil extracellular traps (NET)/ectosomes/exosomes and trogocytosis (membrane exchange) with neighboring cells for modulating innate, and adaptive immune responses. Besides, PMNs exhibit potential heterogeneity and plasticity in involving antibody-dependent cellular cytotoxicity (ADCC), cancer immunity, autoimmunity, inflammatory rheumatic diseases, and cardiovascular diseases. Interestingly, PMNs may also play a role in ameliorating inflammatory reaction and wound healing by a subset of PMN myeloid-derived suppressor cells (PMN-MDSC). Furthermore, PMNs can interact with other non-immune cells including platelets, epithelial and endothelial cells to link hemostasis, mucosal inflammation, and atherogenesis. The release of low-density granulocytes (LDG) from bone marrow initiates systemic autoimmune reaction in systemic lupus erythematosus (SLE). In clinical application, identification of certain PMN phenotypes may become prognostic factors for severe traumatic patients. In the present review, we will discuss these newly discovered biological and pathobiological functions of the PMNs.
An Autonomous Platform for Near Real-Time Surveillance of Harmful Algae and Their Toxins in Dynamic Coastal Shelf Environments
Authors: Moore Stephanie K. , Mickett John B. , Doucette Gregory J. , Adams Nicolaus G. , Mikulski Christina M. , Birch James M. , Roman Brent , Michel-Hart Nicolas , Newton Jan A.
Published: March 2021

Efforts to identify in situ the mechanisms underpinning the response of harmful algae to climate change demand frequent observations in dynamic and often difficult to access marine and freshwater environments. Increasingly, resource managers and researchers are looking to fill this data gap using unmanned systems. In this study we integrated the Environmental Sample Processor (ESP) into an autonomous platform to provide near real-time surveillance of harmful algae and the toxin domoic acid on the Washington State continental shelf over a three-year period (2016–2018). The ESP mooring design accommodated the necessary subsystems to sustain ESP operations, supporting deployment durations of up to 7.5 weeks. The combination of ESP observations and a suite of contextual measurements from the ESP mooring and a nearby surface buoy permitted an investigation into toxic Pseudo-nitzschia spp. bloom dynamics. Preliminary findings suggest a connection between bloom formation and nutrient availability that is modulated by wind-forced coastal-trapped waves. In addition, high concentrations of Pseudo-nitzschia spp. and elevated levels of domoic acid observed at the ESP mooring location were not necessarily associated with the advection of water from known bloom initiation sites. Such insights, made possible by this autonomous technology, enable the formulation of testable hypotheses on climate-driven changes in HAB dynamics that can be investigated during future deployments.
Numerical Computation of Dusty Hybrid Nanofluid Flow and Heat Transfer over a Deformable Sheet with Slip Effect
Authors: Anuar Nur Syazana , Bachok Norfifah , Pop Ioan
Published: March 2021

The mathematical modeling of dusty Cu-Al2O3/water nanofluid flow driven by a permeable deformable sheet was explored numerically. Rather than no–slip conditions at the boundary, velocity slip and thermal slip were considered. To achieve the system of nonlinear ordinary differential equations (ODEs), we employed some appropriate transformations and solved them numerically using MATLAB software (built–in solver called bvp4c). The influences of relevant parameters on fluid flow and heat transfer characteristics are discussed and presented in graphs. The findings showed that double solutions appeared in the case of stretching and shrinking sheets which contributed to the analysis of stability. The stability analysis, therefore, confirmed that merely the first solution was a stable solution. The addition of nanometer-sized particles (Cu) was found to significantly strengthen the heat transfer rate of the dusty nanofluid. Meanwhile, an upsurge in the velocity and thermal slip was shown to decrease the local Nusselt number. The result also revealed that an increment of fluid particle interaction decreased the boundary layer thickness.
Predicting PM2.5 and PM10 Levels during Critical Episodes Management in Santiago, Chile, with a Bivariate Birnbaum-Saunders Log-Linear Model
Authors: Puentes Rodrigo , Marchant Carolina , Leiva Víctor , Figueroa-Zúñiga Jorge I. , Ruggeri Fabrizio
Published: March 2021

Improving air quality is an important environmental challenge of our time. Chile currently has one of the most stable and emerging economies in Latin America, where human impact on natural resources and air quality does not go unperceived. Santiago, the capital of Chile, is one of the cities in which particulate matter (PM) levels exceed national and international limits. Its location and climate cause critical conditions for human health when interaction with anthropogenic emissions is present. In this paper, we propose a predictive model based on bivariate regression to estimate PM levels, related to PM2.5 and PM10, simultaneously. Birnbaum-Saunders distributions are used in the joint modeling of real-world PM2.5 and PM10 data by considering as covariates some relevant meteorological variables employed in similar studies. The Mahalanobis distance is utilized to assess bivariate outliers and to detect suitability of the distributional assumption. In addition, we use the local influence technique for analyzing the impact of a perturbation on the overall estimation of model parameters. In the predictions, we check the categorization for the observed and predicted cases of the model according to the primary air quality regulations for PM.
Farmed Gilthead Sea Bream (Sparus aurata) by-Products Valorization: Viscera Oil ω-3 Enrichment by Short-Path Distillation and In Vitro Bioactivity Evaluation
Authors: Messina Concetta Maria , Arena Rosaria , Manuguerra Simona , Renda Giuseppe , Laudicella Vincenzo Alessandro , Ficano Giovanna , Fazio Gioacchino , La Barbera Laura , Santulli Andrea
Published: March 2021

This study shows a pilot scale protocol aimed to obtain an omega 3-enriched oil after the processing of farmed gilthead sea bream viscera (SBV); this was oil was tested in vitro for bioactivity, attesting to the possibility to turn waste into profit The quality of the oil, in terms of requirements for animal and human consumption, was assessed by determining some chemical parameters, such as peroxide value (PV), thiobarbituric acid reactive substances (TBARS), ρ-anisidine (ρ-AV) content, total oxidation value (TOTOX), and phospholipids and free fatty acid (%), both in crude viscera oil (CVO) and refined viscera oil (RVO). Among the extraction conditions, the higher CVO yields were obtained at 60 °C for 10 min (57.89%) and at 80 °C for 10 min (67.5%), and the resulting oxidation levels were low when utilizing both extraction conditions. RVO, obtained from CVO extracted at 60 °C, showed the highest quality on the basis of the assessed parameters. The ethyl esters of the total fatty acid (TFA) contents extracted from RVO were enriched in the ω-3 polyunsaturated fatty acid fraction (PUFAE) up to almost 56% via short path distillation (SPD). Antioxidant activities and adipogenic properties were tested in vitro. PUFAE protected 3T3 L1 cells from oxidative stress and exerted an anti-adipogenic effect in Dicentrarchus labrax pre-adipocytes, attesting to the beneficial properties for both farmed fish and human health. These results could stimulate the adoption of solutions aimed to recover and utilize aquaculture by-products at a higher scale, turning “waste into profit” and indicating a strategy to reach more sustainable business models in aquaculture resource utilization according to the principles of the circular economy.
Implantable NMR Microcoils in Rats: A New Tool for Exploring Tumor Metabolism at Sub-Microliter Scale?
Authors: Deborne Justine , Pinaud Noël , Crémillieux Yannick
Published: March 2021

The aim of this study was to evaluate the potential of a miniaturized implantable nuclear magnetic resonance (NMR) coil to acquire in vivo proton NMR spectra in sub-microliter regions of interest and to obtain metabolic information using magnetic resonance spectroscopy (MRS) in these small volumes. For this purpose, the NMR microcoils were implanted in the right cortex of healthy rats and in C6 glioma-bearing rats. The dimensions of the microcoil were 450 micrometers wide and 3 mm long. The MRS acquisitions were performed at 7 Tesla using volume coil for RF excitation and microcoil for signal reception. The detection volume of the microcoil was measured equal to 450 nL. A gain in sensitivity equal to 76 was found in favor of implanted microcoil as compared to external surface coil. Nine resonances from metabolites were assigned in the spectra acquired in healthy rats (n = 5) and in glioma-bearing rat (n = 1). The differences in relative amplitude of choline, lactate and creatine resonances observed in glioma-bearing animal were in agreement with published findings on this tumor model. In conclusion, the designed implantable microcoil is suitable for in vivo MRS and can be used for probing the metabolism in localized and very small regions of interest in a tumor.
From Dysbiosis to Healthy Skin: Major Contributions of Cutibacterium acnes to Skin Homeostasis
Authors: Rozas Miquel , Hart de Ruijter Astrid , Fabrega Maria Jose , Zorgani Amine , Guell Marc , Paetzold Bernhard , Brillet Francois
Published: March 2021

Cutibacterium acnes is the most abundant bacterium living in human, healthy and sebum-rich skin sites, such as the face and the back. This bacterium is adapted to this specific environment and therefore could have a major role in local skin homeostasis. To assess the role of this bacterium in healthy skin, this review focused on (i) the abundance of C. acnes in the skin microbiome of healthy skin and skin disorders, (ii) its major contributions to human skin health, and (iii) skin commensals used as probiotics to alleviate skin disorders. The loss of C. acnes relative abundance and/or clonal diversity is frequently associated with skin disorders such as acne, atopic dermatitis, rosacea, and psoriasis. C. acnes, and the diversity of its clonal population, contributes actively to the normal biophysiological skin functions through, for example, lipid modulation, niche competition and oxidative stress mitigation. Compared to gut probiotics, limited dermatological studies have investigated skin probiotics with skin commensal strains, highlighting their unexplored potential.
An Actinobacterial Isolate, Streptomyces sp. YX44, Produces Broad-Spectrum Antibiotics That Strongly Inhibit Staphylococcus aureus
Authors: Chang Tien-Lin , Huang Tzu-Wen , Wang Ying-Xuan , Liu Chang-Pan , Kirby Ralph , Chu Chien-Ming , Huang Chih-Hung
Published: March 2021

The need for new antibiotics is increasing due to their overuse, and antibiotic resistance has become one of the major threats worldwide to public health, food safety, and clinical treatment. In this study, we describe an actinobacterial isolate, YX44, which belongs to the genus Streptomyces. This Streptomyces was isolated from a drinking pipe located in Osaka, Japan, and has the ability to inhibit Gram-positive bacteria, Gram-negative bacteria, and various fungi. YX44 fermentation broth shows strong activity against Escherichia coli and Staphylococcus aureus, as well as also inhibiting clinical isolates of multidrug-resistant Staphylococcus aureus. The YX44 antibacterial substances in the broth are relatively heat-stable, show high stability from the pH range 1 to 11, and have good solubility in both organic and non-organic solvents. Size-exclusion chromatography revealed that the YX44 antibacterial compounds are less than 1000 Da in size. LC-MS was able to identify three possible candidate molecules with molecular weights of 308, 365, 460, and 653 g/mol; none of these sizes correspond to any well-known antibiotics. Our results show that Streptomyces sp. YX44 seems to produce a number of novel antibiotics with high pH stability and good solubility that have significant activity against S. aureus, including multidrug-resistant strains.
Comprehensive Investigation on Ginsenosides in Different Parts of a Garden-Cultivated Ginseng Root and Rhizome
Authors: Pan Junqian , Zheng Wei , Pang Xu , Zhang Jie , Chen Xiaojuan , Yuan Ming , Yu Kate , Guo Baolin , Ma Baiping
Published: March 2021

Background: Ginseng is widely used as herb or food. Different parts of ginseng have diverse usages. However, the comprehensive analysis on the ginsenosides in different parts of ginseng root is scarce. Methods: An ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) combined with UNIFI informatics platform and ultra-high-performance liquid chromatography-charged aerosol detection (UHPLC-CAD) were employed to evaluate the different parts of cultivated ginseng root. Results: 105 ginsenosides including 16 new compounds were identified or tentatively characterized. 22 potential chemical markers were identified, 20, 17, and 19 for main root (MR) and fibrous root (FR), main root (MR) and branch root (BR), and main root (MR) and rhizome (RH), respectively. The relative contents of Re, Rb1, 20(R)-Rh1, Rd, and Rf were highest in FR. The relative content of Rg1 was highest in RH. The total relative content of pharmacopoeia indicators Rg1, Re, and Rb1 was highest in FR. Conclusion: The differences among these parts were the compositions and relative contents of ginsenosides. Under our research conditions, the peak area ratio of Rg1 and Re could distinguish the MR and FR samples. Fibrous roots showed rich ingredients and high ginsenosides contents which should be further utilized.
S-allyl Cysteine Enhances Testosterone Production in Mice and Mouse Testis-Derived I-10 Cells
Authors: Rana Md Masud , Shiozawa Kota , Mukai Katsuyuki , Takayanagi Katsuhiko , Eguchi Koichi , Sultana Halima , Ohsaki Yusuke , Komai Michio , Shirakawa Hitoshi
Published: March 2021

Hypogonadism, associated with low levels of testosterone synthesis, has been implicated in several diseases. Recently, the quest for natural alternatives to prevent and treat hypogonadism has gained increasing research interest. To this end, the present study explored the effect of S-allyl cysteine (SAC), a characteristic organosulfur compound in aged-garlic extract, on testosterone production. SAC was administered at 50 mg/kg body weight intraperitoneally into 7-week-old BALB/c male mice in a single-dose experiment. Plasma levels of testosterone and luteinizing hormone (LH) and testis levels of proteins involved in steroidogenesis were measured by enzymatic immunoassay and Western blot, respectively. In addition, mouse testis-derived I-10 cells were also used to investigate the effect of SAC on steroidogenesis. In the animal experiment, SAC significantly elevated testosterone levels in both the plasma and the testis without changing the LH level in plasma and increased phosphorylated protein kinase A (p-PKA) levels. Similar results were also observed in I-10 cells. The findings demonstrating the increasing effect of SAC on p-PKA and mRNA levels of Cyp11a suggest that SAC increases the testosterone level by activating the PKA pathway and could be a potential target for hypogonadism therapeutics.
Sunscreens Containing Cyclodextrin Inclusion Complexes for Enhanced Efficiency: A Strategy for Skin Cancer Prevention
Authors: Dahabra Layan , Broadberry Grace , Le Gresley Adam , Najlah Mohammad , Khoder Mouhamad
Published: March 2021

Unprotected exposure of skin to solar ultraviolet radiation (UVR) may damage the DNA of skin cells and can lead to skin cancer. Sunscreens are topical formulations used to protect skin against UVR. The active ingredients of sunscreens are UV filters that absorb, scatter, and/or reflect UVR. Preventing the formation of free radicals and repairing DNA damages, natural antioxidants are also added to sunscreens as a second fold of protection against UVR. Antioxidants can help stabilise these formulations during the manufacturing process and upon application on skin. However, UV filters and antioxidants are both susceptible to degradation upon exposure to sunlight and oxygen. Additionally, due to their poor water solubility, natural antioxidants are challenging to formulate and exhibit limited penetration and bioavailability in the site of action (i.e., deeper skin layers). Cyclodextrins (CDs) are cyclic oligosaccharides that are capable of forming inclusion complexes with poorly soluble drugs, such as antioxidants. In this review, we discuss the use of CDs inclusion complexes to enhance the aqueous solubility of antioxidants and chemical UV filters and provide a protective shield against degradative factors. The role of CDs in providing a controlled drug release profile from sunscreens is also discussed. Finally, incorporating CDs inclusion complexes into sunscreens has the potential to increase their efficiency and hence improve their skin cancer prevention.
Selenium Alleviates the Adverse Effect of Drought in Oilseed Crops Camelina (Camelina sativa L.) and Canola (Brassica napus L.)
Authors: Ahmad Zahoor , Anjum Shazia , Skalicky Milan , Waraich Ejaz Ahmad , Muhammad Sabir Tariq Rana , Ayub Muhammad Ashar , Hossain Akbar , Hassan Mohamed M. , Brestic Marian , Sohidul Islam Mohammad , Habib-Ur-Rahman Muhammad , Wasaya Allah , Aamir Iqbal Muhammad , EL Sabagh Ayman
Published: March 2021

Drought poses a serious threat to oilseed crops by lowering yield and crop failures under prolonged spells. A multi-year field investigation was conducted to enhance the drought tolerance in four genotypes of Camelina and canola by selenium (Se) application. The principal aim of the research was to optimize the crop yield by eliciting the physio-biochemical attributes by alleviating the adverse effects of drought stress. Both crops were cultivated under control (normal irrigation) and drought stress (skipping irrigation at stages i.e., vegetative and reproductive) conditions. Four different treatments of Se viz., seed priming with Se (75 μM), foliar application of Se (7.06 μM), foliar application of Se + Seed priming with Se (7.06 μM and 75 μM, respectively) and control (without Se), were implemented at the vegetative and reproductive stages of both crops. Sodium selenite (Na2SeO3), an inorganic compound was used as Se sources for both seed priming and foliar application. Data regarding physiochemical, antioxidants, and yield components were recorded as response variables at crop maturity. Results indicated that WP, OP, TP, proline, TSS, TFAA, TPr, TS, total chlorophyll contents, osmoprotectant (GB, anthocyanin, TPC, and flavonoids), antioxidants (APX, SOD, POD, and CAT), and yield components (number of branches per plant, thousand seed weight, seed, and biological yields were significantly improved by foliar Se + priming Se in both crops under drought stress. Moreover, this treatment was also helpful in boosting yield attributes under irrigated (non-stress) conditions. Camelina genotypes responded better to Se application as seed priming and foliar spray than canola for both years. It has concluded that Se application (either foliar or priming) can potentially alleviate adverse effects of drought stress in camelina and canola by eliciting various physio-biochemicals attributes under drought stress. Furthermore, Se application was also helpful for crop health under irrigated condition.
Phase Behavior of Aqueous Biphasic Systems with Choline Alkanoate Ionic Liquids and Phosphate Solutions: The Influence of pH
Authors: Berton Paula , Tian Hongzhe , Rogers Robin D.
Published: March 2021

Aqueous biphasic systems (ABS) composed of the choline alkanoate ionic liquids (ILs) choline acetate [Cho][OAc], choline propanoate [Cho][Pro], choline butyrate [Cho][But], and choline hexanoate [Cho][Hex], mixed with K3PO4 solutions at pH 7.2 and 14.5, were prepared and their phase diagrams were compared. The ability to form ABS with alkaline K3PO4 solutions decreased in the order [Cho][OAc] ≈ [Cho][Pro] > [Cho][But] > [Cho][Hex], while with neutral K3PO4 solutions, [Cho][OAc] could not form an ABS, and the other three ILs performed similarly. All of the biphasic regions of the ABS decreased with the increase in pH. 1H-NMR data indicated anion exchange between phases in ABS at neutral pH. The ABS at neutral pH were evaluated to extract the triazine herbicides simazine, cyanazine, and atrazine, and the ABS formed by [Cho][Pro] and the pH 7.2 K3PO4 solution has shown extraction recoveries higher than 90%.
Synthesis, Molecular Docking Studies and In Silico ADMET Screening of New Heterocycles Linked Thiazole Conjugates as Potent Anti-Hepatic Cancer Agents
Authors: Rashdan Huda R. M. , El-Naggar Mohamed , Abdelmonsef Aboubakr H.
Published: March 2021

Thiazoles are important scaffolds in organic chemistry. Biosynthesis of thiazoles is considered to be an excellent target for the design of novel classes of therapeutic agents. In this study, a new series of 2-ethylidenehydrazono-5-arylazothiazoles 5a–d and 2-ethylidenehydrazono-5-arylazo- thiazolones 8a–d were synthesized via the cyclocondensation reaction of the appropriate hydrazonyl halides 4a–d and 7a–d with ethylidene thiosemicarbazide 3, respectively. Furthermore, the thiosemicarbazide derivative 3 was reacted with different bromoacetyl compounds 10–12 to afford the respective thiazole derivatives 13–15. Chemical composition of the novel derivatives was established on bases of their spectral data (FTIR, 1H-NMR, 13C-NMR and mass spectrometry) and microanalytical data. The newly synthesized derivatives were screened for their in vitro anti-hepatic cancer potency using an MTT assay. Moreover, an in silico technique was used to assess the interaction modes of the compounds with the active site of Rho6 protein. The docking studies of the target Rho6 with the newly synthesized fourteen compounds showed good docking scores with acceptable binding interactions. The presented results revealed that the newly synthesized compounds exhibited promising inhibition activity against hepatic cancer cell lines (HepG2).
Enzymatic Saccharification with Sequential-Substrate Feeding and Sequential-Enzymes Loading to Enhance Fermentable Sugar Production from Sago Hampas
Authors: Alias Nurul Haziqah , Abd-Aziz Suraini , Yee Phang Lai , Ibrahim Mohamad Faizal
Published: March 2021

Sago hampas composed of a high percentage of polysaccharides (starch, cellulose and hemicellulose) that make it a suitable substrate for fermentation. However, the saccharification of sago hampas through the batch process is always hampered by its low sugar concentration due to the limitation of the substrate that can be loaded into the system. Increased substrate concentration in the system reduces the ability of enzyme action toward the substrate due to substrate saturation, which increases viscosity and causes inefficient mixing. Therefore, sequential-substrate feeding has been attempted in this study to increase the amount of substrate in the system by feeding the substrate at the selected intervals. At the same time, sequential-enzymes loading has been also evaluated to maximize the amount of enzymes loaded into the system. Results showed that this saccharification with sequential-substrate feeding and sequential-enzymes loading has elevated the solid loading up to 20% (w/v) and reduced the amount of enzymes used per substrate input by 20% for amylase and 50% for cellulase. The strategies implemented have enhanced the fermentable sugar production from 80.33 g/L in the batch system to 119.90 g/L in this current process. It can be concluded that sequential-substrate feeding and sequential-enzymes loading are capable of increasing the total amount of substrate, the amount of fermentable sugar produced, and at the same time maximize the amount of enzymes used in the system. Hence, it would be a promising solution for both the economic and waste management of the sago hampas industry to produce value-added products via biotechnological means.
Human Body-Related Disease Diagnosis Systems Using CMOS Image Sensors: A Systematic Review
Authors: Sukhavasi Suparshya Babu , Sukhavasi Susrutha Babu , Elleithy Khaled , Abuzneid Shakour , Elleithy Abdelrahman
Published: March 2021

According to the Center for Disease Control and Prevention (CDC), the average human life expectancy is 78.8 years. Specifically, 3.2 million deaths are reported yearly due to heart disease, cancer, Alzheimer’s disease, diabetes, and COVID-19. Diagnosing the disease is mandatory in the current way of living to avoid unfortunate deaths and maintain average life expectancy. CMOS image sensor (CIS) became a prominent technology in assisting the monitoring and clinical diagnosis devices to treat diseases in the medical domain. To address the significance of CMOS image ‘sensors’ usage in disease diagnosis systems, this paper focuses on the CIS incorporated disease diagnosis systems related to vital organs of the human body like the heart, lungs, brain, eyes, intestines, bones, skin, blood, and bacteria cells causing diseases. This literature survey’s main objective is to evaluate the ‘systems’ capabilities and highlight the most potent ones with advantages, disadvantages, and accuracy, that are used in disease diagnosis. This systematic review used PRISMA workflow for study selection methodology, and the parameter-based evaluation is performed on disease diagnosis systems related to the human body’s organs. The corresponding CIS models used in systems are mapped organ-wise, and the data collected over the last decade are tabulated.
Deep Learning-Based Adaptive Neural-Fuzzy Structure Scheme for Bearing Fault Pattern Recognition and Crack Size Identification
Authors: Piltan Farzin , Duong Bach Phi , Kim Jong-Myon
Published: March 2021

Bearings are complex components with onlinear behavior that are used to mitigate the effects of inertia. These components are used in various systems, including motors. Data analysis and condition monitoring of the systems are important methods for bearing fault diagnosis. Therefore, a deep learning-based adaptive neural-fuzzy structure technique via a support vector autoregressive-Laguerre model is presented in this study. The proposed scheme has three main steps. First, the support vector autoregressive-Laguerre is introduced to approximate the vibration signal under normal conditions and extract the state-space equation. After signal modeling, an adaptive neural-fuzzy structure observer is designed using a combination of high-order variable structure techniques, the support vector autoregressive-Laguerre model, and adaptive neural-fuzzy inference mechanism for normal and abnormal signal estimation. The adaptive neural-fuzzy structure observer is the main part of this work because, based on the difference between signal estimation accuracy, it can be used to identify faults in the bearings. Next, the residual signals are generated, and the signal conditions are detected and identified using a convolution neural network (CNN) algorithm. The effectiveness of the proposed deep learning-based adaptive neural-fuzzy structure technique by support vector autoregressive-Laguerre model was analyzed using the Case Western Reverse University (CWRU) bearing vibration dataset. The proposed scheme is compared to five state-of-the-art techniques. The proposed algorithm improved the average pattern recognition and crack size identification accuracy by 1.99%, 3.84%, 15.75%, 5.87%, 30.14%, and 35.29% compared to the combination of the high-order variable structure technique with the support vector autoregressive-Laguerre model and CNN, the combination of the variable structure technique with the support vector autoregressive-Laguerre model and CNN, the combination of RAW signal and CNN, the combination of the adaptive neural-fuzzy structure technique with the support vector autoregressive-Laguerre model and support vector machine (SVM), the combination of the high-order variable structure technique with the support vector autoregressive-Laguerre model and SVM, and the combination of the variable structure technique with the support vector autoregressive-Laguerre model and SVM, respectively.
Decoration of CuO NWs Gas Sensor with ZnO NPs for Improving NO2 Sensing Characteristics
Authors: Han Tae-Hee , Bak So-Young , Kim Sangwoo , Lee Se Hyeong , Han Ye-Ji , Yi Moonsuk
Published: March 2021

This paper introduces a method for improving the sensitivity to NO2 gas of a p-type metal oxide semiconductor gas sensor. The gas sensor was fabricated using CuO nanowires (NWs) grown through thermal oxidation and decorated with ZnO nanoparticles (NPs) using a sol-gel method. The CuO gas sensor with a ZnO heterojunction exhibited better sensitivity to NO2 gas than the pristine CuO gas sensor. The heterojunction in CuO/ZnO gas sensors caused a decrease in the width of the hole accumulation layer (HAL) and an increase in the initial resistance. The possibility to influence the width of the HAL helped improve the NO2 sensing characteristics of the gas sensor. The growth morphology, atomic composition, and crystal structure of the gas sensors were analyzed using field-emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy, and X-ray diffraction, respectively.
A LiDAR Sensor-Based Spray Boom Height Detection Method and the Corresponding Experimental Validation
Authors: Dou Hanjie , Wang Songlin , Zhai Changyuan , Chen Liping , Wang Xiu , Zhao Xueguan
Published: March 2021

Sprayer boom height (Hb) variations affect the deposition and distribution of droplets. An Hb control system is used to adjust Hb to maintain an optimum distance between the boom and the crop canopy, and an Hb detection sensor is a key component of the Hb control system. This study presents a new, low-cost light detection and ranging (LiDAR) sensor for Hb detection developed based on the principle of single-point ranging. To examine the detection performance of the LiDAR sensor, a step height detection experiment, a field ground detection experiment, and a wheat stubble (WS) height detection experiment as well as a comparison with an ultrasonic sensor were performed. The results showed that the LiDAR sensor could be used to detect Hb. When used to detect the WS height (HWS), the LiDAR sensor primarily detected the WS roots and the inside of the WS canopy. HWS and movement speed of the LiDAR sensor (VLiDAR) has a greater impact on the detection performance of the LiDAR sensor for the WS canopy than that for the WS roots. The detection error of the LiDAR sensor for the WS roots is less than 5.00%, and the detection error of the LiDAR sensor for the WS canopy is greater than 8.00%. The detection value from the LiDAR sensor to the WS root multiplied by 1.05 can be used as a reference basis for adjusting Hb, and after the WS canopy height is added to the basis, the value can be used as an index for adjusting Hb in WS field spraying. The results of this study will promote research on the boom height detection method and autonomous Hb control system.
Remote Photoacoustic Sensing Using Single Speckle Analysis by an Ultra-Fast Four Quadrant Photo-Detector
Authors: Lengenfelder Benjamin , Hohmann Martin , Späth Moritz , Scherbaum Daniel , Weiß Manuel , Rupitsch Stefan J. , Schmidt Michael , Zalevsky Zeev , Klämpfl Florian
Published: March 2021

The need for tissue contact makes photoacoustic imaging not applicable for special medical applications like wound imaging, endoscopy, or laser surgery. An easy, stable, and contact-free sensing technique might thus help to broaden the applications of the medical imaging modality. In this work, it is demonstrated for the first time that remote photoacoustic sensing by speckle analysis can be performed in the MHz sampling range by tracking a single speckle using a four quadrant photo-detector. A single speckle, which is created by self-interference of surface back-reflection, is temporally analyzed using this photo-detector. Phantoms and skin samples are measured in transmission and reflection mode. The potential for miniaturization for endoscopic application is demonstrated by fiber bundle measurements. In addition, sensing parameters are discussed. Photoacoustic sensing in the MHz sampling range by single speckle analysis with the four quadrant detector is successfully demonstrated. Furthermore, the endoscopic applicability is proven, and the sensing parameters are convenient for photoacoustic sensing. It can be concluded that a single speckle contains all the relevant information for remote photoacoustic signal detection. Single speckle sensing is therefore an easy, robust, contact-free photoacoustic detection technique and holds the potential for economical, ultra-fast photoacoustic sensing. The new detection technique might thus help to broaden the field of photoacoustic imaging applications in the future.
Constructionist Learning Tool for Acquiring Skills in Understanding Standardised Engineering Drawings of Mechanical Assemblies in Mobile Devices
Authors: Fraile-Fernández Fernando J. , Martínez-García Rebeca , Castejón-Limas Manuel
Published: March 2021

The purpose of Graphic Design is to transfer information about design into reality and concerns the analysis, design and representation of mechanical components and assemblies. For the correct rendering of mechanical components, this discipline requires a command of, and the ability to, manage techniques and systems for graphical representation and standardisation; 3D models in a virtual environment enable engineering students to develop graphical skills and spatial awareness. The objective of the present study is the development of an application for smart devices (mobile phones and tablets), based on the constructionist theory of learning, which will enable first year engineering degree students to acquire the technical drawing knowledge and skills necessary to render mechanical assemblies. The mobile application tested and designed in this work is called ARPAID. It is a learning tool aimed at teaching students about the representation of mechanical assemblies as part of an engineering Graphic Design course. Teaching material and a process for evaluation have been designed. A detailed description is given of a classroom activity accompanied by a tabulation and analysis of the results obtained. This mobile application, when used in a Graphic Design course, promotes a more rapid understanding of spatial relationships and problems, fosters students’ learning and motivation, and develops higher order skills. Results from before and after the use of the application will be presented and do indeed show significant improvements in student performance.