bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing Speech Rehabilitation in a Young Adult with Trisomy 21: Integrating Transcranial Direct Current Stimulation (tDCS) with Rapid Syllable Transition Training for Apraxia of Speech
Authors: Nakamura-Palacios Ester Miyuki , Falçoni Júnior Aldren Thomazini , Tanese Gabriela Lolli , Vogeley Ana Carla Estellita , Namasivayam Aravind Kumar
Published: January 2024

Apraxia of speech is a persistent speech motor disorder that affects speech intelligibility. Studies on speech motor disorders with transcranial Direct Current Stimulation (tDCS) have been mostly directed toward examining post-stroke aphasia. Only a few tDCS studies have focused on apraxia of speech or childhood apraxia of speech (CAS), and no study has investigated individuals with CAS and Trisomy 21 (T21, Down syndrome). This N-of-1 randomized trial examined the effects of tDCS combined with a motor learning task in developmental apraxia of speech co-existing with T21 (ReBEC RBR-5435x9). The accuracy of speech sound production of nonsense words (NSWs) during Rapid Syllable Transition Training (ReST) over 10 sessions of anodal tDCS (1.5 mA, 25 cm) over Broca’s area with the cathode over the contralateral region was compared to 10 sessions of sham-tDCS and four control sessions in a 20-year-old male individual with T21 presenting moderate–severe childhood apraxia of speech (CAS). The accuracy for NSW production progressively improved (gain of 40%) under tDCS (sham-tDCS and control sessions showed < 20% gain). A decrease in speech severity from moderate–severe to mild–moderate indicated transfer effects in speech production. Speech accuracy under tDCS was correlated with Wernicke’s area activation (P3 current source density), which in turn was correlated with the activation of the left supramarginal gyrus and the Sylvian parietal–temporal junction. Repetitive bihemispheric tDCS paired with ReST may have facilitated speech sound acquisition in a young adult with T21 and CAS, possibly through activating brain regions required for phonological working memory.
Understanding the Neurotrophic Virus Mechanisms and Their Potential Effect on Systemic Lupus Erythematosus Development
Authors: Uribe Felipe R. , González Valentina P. I. , Kalergis Alexis M. , Soto Jorge A. , Bohmwald Karen
Published: January 2024

Central nervous system (CNS) pathologies are a public health concern, with viral infections one of their principal causes. These viruses are known as neurotropic pathogens, characterized by their ability to infiltrate the CNS and thus interact with various cell populations, inducing several diseases. The immune response elicited by neurotropic viruses in the CNS is commanded mainly by microglia, which, together with other local cells, can secrete inflammatory cytokines to fight the infection. The most relevant neurotropic viruses are adenovirus (AdV), cytomegalovirus (CMV), enterovirus (EV), Epstein–Barr Virus (EBV), herpes simplex virus type 1 (HSV-1), and herpes simplex virus type 2 (HSV-2), lymphocytic choriomeningitis virus (LCMV), and the newly discovered SARS-CoV-2. Several studies have associated a viral infection with systemic lupus erythematosus (SLE) and neuropsychiatric lupus (NPSLE) manifestations. This article will review the knowledge about viral infections, CNS pathologies, and the immune response against them. Also, it allows us to understand the relevance of the different viral proteins in developing neuronal pathologies, SLE and NPSLE.
Academic Self-Concept Wins the Race: The Prediction of Achievements in Three Major School Subjects by Five Subject-Specific Self-Related Variables
Authors: Rost Detlef H. , Feng Xiaoli
Published: January 2024

The importance of self-related constructs in predicting academic achievement has been increasingly emphasized in recent decades. Typically, bivariate associations of self-related variables with achievements have been reported. Research quantifying the combined predictive power of more than two self-variables has been scarce. Moreover, except for the academic self-concept, these variables have almost always been measured across domains, i.e., without considering the specifics of individual school subjects. The current study aimed to statistically predict academic achievement (operationalized via school grades) in three major subjects (Chinese (native language), mathematics, and English (foreign language)) by using subject-tied scales, namely academic self-concept, conscientiousness, need for cognition, perseverance of effort, and consistency of interest. The sample comprised 791 Chinese adolescents. Each scale was related separately to each of the three school subjects. Hierarchical linear regression analyses were run. The control variable, biological sex, accounted for 2% of Chinese grades and 8% of English grades, but not of mathematics grades. Adding subject-specific self-concept scales increased the explained variance to 7% (Chinese), 16% (mathematics), and 32% (English). Further additions to the other four self-related scales did not increase the variances that were accounted for. The discussion underlines the relevance of subject-specific academic self-concepts as predictors for subject-tied academic achievements.
Flexibility Retained: Unimpaired Updating of Expectations in Schizophrenia
Authors: Li Jian , Chen Luo , Zhou Dongsheng , Tang Enze , Zheng Jiewei , Huang Xiaoqi , Zhong Bao-Liang , Guan Chenxiao , Liu Huiying , Shen Mowei , Chen Hui
Published: January 2024

Flexibly and actively updating expectations based on feedback is crucial for navigating daily life. Previous research has shown that people with schizophrenia (PSZ) have difficulty adjusting their expectations. However, there are studies suggesting otherwise. To explore this further, we used a novel trial-based expectation updating paradigm called attribute amnesia. In the task, the participants needed to report the location of a target stimulus among distractors in pre-surprise trials. In the surprise trial, they were unexpectedly asked to report the identity of the target before reporting its location. Afterward, control trials were conducted whereby the participants were asked the same questions as in the surprise trial. Notably, the surprise trial and control trials were nearly identical, except that the participants expected to be asked about identity information in the control trials but not in the surprise trial. Thus, an improvement in identity reporting accuracy in the control trials in comparison with the surprise trial indicated active updating of expectations. In the current study, a total of 63 PSZ and 60 healthy control subjects (HCS) were enrolled. We found that both the PSZ and the HCS were unable to report information that they had fully attended to (i.e., identity) in the surprise trial. However, both groups showed a significant improvement in reporting identity information even in the first control trial. Critically, there was no significant difference in the magnitude of improvement between the two groups. The current findings indicate that PSZ have the ability to update their expectations as quickly and flexibly as HCS, at least in the context of the current task. The possible factors that might contribute to the discrepancy regarding expectation updating are discussed.
Performance Evaluation of Inerter-Based Dynamic Vibration Absorbers for Wind-Induced Vibration Control of a Desulfurization Tower
Authors: Li Yang , Zhang Qinghua , Xu Yanwei , Wen Jinlong , Wang Zhihao
Published: January 2024

High-rise flue gas desulfurization towers are susceptible to wind loads, which can cause instability and failure in the along-wind and across-wind directions. The tuned mass damper (TMD) has been widely applied in the wind-induced vibration control of high-rise structures. To enhance the control performance and reduce the auxiliary mass of TMD, this study focuses on inerter-based dynamic vibration absorbers (IDVAs) for controlling the vibration response of a desulfurization tower. The dynamical equations of the tower–IDVA systems are established under wind loads, and a parameter optimization strategy for IDVAs is proposed by using the genetic algorithm. The performance of the traditional TMD and six IDVAs in the vibration control of the tower are systematically compared. Numerical simulations demonstrate that both the TMD and IDVAs can substantially mitigate the vibration response of the tower. However, compared to the TMD with the same response mitigation ratio, more than 34% of the auxiliary mass can be reduced by two optimal IDVAs. In addition, the energy dissipation enhancement and lightweight effect of the two IDVAs are explained through parametric studies.
A Review of Advances in Graphene Quantum Dots: From Preparation and Modification Methods to Application
Authors: Cui Yibo , Liu Luoyi , Shi Mengna , Wang Yuhao , Meng Xiaokai , Chen Yanjun , Huang Que , Liu Changcheng
Published: January 2024

Graphene quantum dot (GQD) is a new type of carbon nanometer material. In addition to the excellent properties of graphene, it is superior due to the quantum limit effect and edge effect. Because of its advantages such as water solution, strong fluorescent, small size, and low biological toxicity, it has important application potential in various fields, especially in sensors and biomedical areas, which are mainly used as optical electrical sensors as well as in biological imaging and tumor therapy. In addition, GQDs have very important characteristics, such as optical and electrical properties. There are many preparation methods, divided into top-down and bottom-up methods, which have different advantages and disadvantages, respectively. In addition, the modification methods include heterogeneous doping, surface heterogeneity, etc. There are still many challenges in developing GQDs. For example, the synthesis steps are still hard to conduct, but as the inquiry continues to deepen, GQDs will be revolutionary materials in the future. In this work, the literature concerning research progress on GQDs has been reviewed and summarized, while the key challenges of their application have been pointed out, which may bring new insights to the application of GQDs.
Modulation of Cisplatin Sensitivity through TRPML1-Mediated Lysosomal Exocytosis in Ovarian Cancer Cells: A Comprehensive Metabolomic Approach
Authors: Kim Boyun , Kim Gaeun , Kim Heeyeon , Song Yong Sang , Jung Jewon
Published: January 2024

Background: The lysosome has emerged as a promising target for overcoming chemoresistance, owing to its role in facilitating the lysosomal sequestration of drugs. The lysosomal calcium channel TRPML1 not only influences lysosomal biogenesis but also coordinates both endocytosis and exocytosis. This study explored the modulation of cisplatin sensitivity by regulating TRPML1-mediated lysosomal exocytosis and identified the metabolomic profile altered by TRPML1 inhibition. Methods: We used four types of ovarian cancer cells: two cancer cell lines (OVCAR8 and TOV21G) and two patient-derived ovarian cancer cells. Metabolomic analyses were conducted to identify altered metabolites by TRPML1 inhibition. Results: Lysosomal exocytosis in response to cisplatin was observed in resistant cancer cells, whereas the phenomenon was absent in sensitive cancer cells. Through the pharmacological intervention of TRPML1, lysosomal exocytosis was interrupted, leading to the sensitization of resistant cancer cells to cisplatin treatment. To assess the impact of lysosomal exocytosis on chemoresistance, we conducted an untargeted metabolomic analysis on cisplatin-resistant ovarian cancer cells with TRPML1 inhibition. Among the 1446 differentially identified metabolites, we focused on 84 significant metabolites. Metabolite set analysis revealed their involvement in diverse pathways. Conclusions: These findings collectively have the potential to enhance our understanding of the interplay between lysosomal exocytosis and chemoresistance, providing valuable insights for the development of innovative therapeutic strategies.
Analysis of Effectiveness of Combined Surface Treatment Methods for Structural Parts with Holes to Enhance Their Fatigue Life
Authors: Grebenikov Olexander , Humennyi Andrii , Svitlychnyi Serhii , Lohinov Vasyl , Matviienko Valerii
Published: January 2024

The typical and most widespread stress concentrators in the lower wing panels of aircraft are the drain holes located on the stringer vertical ribs. These are prime sources for the initiation and development of fatigue cracks, which lead to early failure of the wing structure. Therefore, improving fatigue life in these critical areas is one of the significant issues for research. Two combined methods of surface plastic treatment in the location around drain holes are discussed in this paper. Using the finite element method and ANSYS software, we created a finite element model and obtained nonlinear solution results in the case of tension in a plate with three holes. In addition, the development of residual stress due to the surface plastic treatment of the hole-adjacent areas was taken into account. In this paper, it is shown that after surface treatment of the corresponding areas of the holes, residual stress, which exceeds the yield stress for the plate material, is induced. When combined with alternative tensile stress, these reduce the amplitude of the local stresses, thus increasing the number of stress cycles before failure. The benefits of this technology were confirmed by fatigue test results, which include the fatigue failure types of the plates. Graphs showing the impact of applicable surface treatment combined methods on the number of cycles to failure were also plotted.
Faraway, so Close: Perceptions of the Metaverse on the Edge of Madness
Authors: Cruz Mónica , Oliveira Abílio , Pinheiro Alessandro
Published: January 2024

With the evolution of technologies, virtual reality allows us to dive into cyberspace through different devices and have immersive experiences in different contexts, which, in a simple way, we call virtual worlds or multiverse (integrating Metaverse versions). Through virtual reality, it is possible to create infinite simulated environments to immerse ourselves in. Future internet may be slightly different from what we use today. Virtual immersion situations are common (particularly in gaming), and the Metaverse has become a lived and almost real experience claiming its presence in our daily lives. To investigate possible perspectives or concepts regarding the Metaverse, virtual reality, and immersion, we considered a main research question: To what extent can a film centered on the multiverse be associated with adults’ Metaverse perceptions? Considering that all participants are adults, the objectives of this study are: (1) Verify the representations of the Metaverse; (2) Verify the representations of immersion; (3) Verify the representations of the multiverse; (4) Verify the importance of a film (related to the Metaverse and the multiverse) on the representations found. This study—framed in a Ph.D. research project—analyzed the participants’ answers through an online survey using two films to gather thoughts, ideas, emotions, sentiments, and reactions according to our research objectives. Some limitations were considered, such as the number of participants, number of the questionnaire questions and the knowledge or lack of the main concepts. Our results showed that a virtual world created by a movie might stimulate the perception of almost living in that supposed reality, accepting the multiverse and Metaverse not as distant concepts but as close experiences, even in an unconscious form. This finding is also a positive contribution to a discussion in progress aiming for an essential understanding of the Metaverse as a complex concept.
ZnO-Au Hybrid Metamaterial Thin Films with Tunable Optical Properties
Authors: Bhatt Nirali A. , Paldi Robynne L. , Barnard James P. , Lu Juanjuan , He Zihao , Yang Bo , Shen Chao , Song Jiawei , Sarma Raktim , Siddiqui Aleem , Wang Haiyan
Published: January 2024

ZnO-Au nanocomposite thin films have been previously reported as hybrid metamaterials with unique optical properties such as plasmonic resonance properties and hyperbolic behaviors. In this study, Au composition in the ZnO-Au nanocomposites has been effectively tuned by target composition variation and thus resulted in microstructure and optical property tuning. Specifically, all the ZnO-Au nanocomposite thin films grown through the pulsed laser deposition (PLD) method show obvious vertically aligned nanocomposite (VAN) structure with the Au nanopillars embedded in the ZnO matrix. Moreover, the average diameter of Au nanopillars increases as Au concentration increases, which also leads to the redshifts in the surface plasmon resonance (SPR) wavelength and changes in the hyperbolic behaviors of the films. As a whole, this work discusses how strain-driven tuning of optical properties and microstructure resulted through a novel Au concentration variation approach which has not been previously attempted in the ZnO-Au thin film system. These highly ordered films present great promise in the areas of sensing, waveguides, and nanophotonics to name a few.
Intramuscular Neural Distribution of the Gluteus Maximus Muscle: Diagnostic Electromyography and Injective Treatments
Authors: Yi Kyu-Ho , Kim Dong Chan , Lee Siyun , Lee Hyung-Jin , Lee Ji-Hyun
Published: January 2024

Introduction: The purpose of this study was to investigate neural patterns within the gluteus maximus (Gmax) muscle to identify optimal EMG placement and injection sites for botulinum toxin and other injectable agents. Methods: This study used 10 fixed and 1 non-fixed adult Korean cadavers. Intramuscular arborization patterns were confirmed in the cranial, middle, and caudal segments of 20 Gmax muscles using Sihler staining. Ultrasound images were obtained from one cadaver, and blue dye was injected using ultrasound guidance to confirm the results. Results: The intramuscular innervation pattern of the Gmax was mostly in the middle part of this muscle. The nerve endings of the Gmax are mainly located in the 40–70% range in the cranial segment, the 30–60% range in the middle segment, and the 40–70% range in the caudal segment. Discussion: Addressing the spasticity of the gluteus maximus requires precise, site-specific botulinum toxin injections. The use of EMG and other injection therapies should be guided by the findings of this study. We propose that these specific sites, which correspond to areas with the densest nerve branches, are the safest and most efficient locations for both botulinum toxin injections and EMG procedures.
Exploring German High School Students’ Conceptual Learning Pathways of Space and Place
Authors: Bienert Natalie
Published: January 2024

Space and place, fundamental concepts in geography, uniquely contribute to the development of students’ geographical thinking. Despite this, these concepts are underrepresented in German geography classrooms, partly due to a distinct lack of empirical evidence concerning the learning processes associated with learning complex geographical concepts. This study employed videography to explore how n = 64 grade twelve students from various school types in Germany develop a more sophisticated understanding of space and place during a 90 min. teaching experiment. To delve into the micro-level of students’ learning processes, qualitative content analysis was employed to analyse the data. The subsequent detailed reconstruction of an individual learning pathway unveiled several pivotal moments. Notably, the concepts demonstrated significant potential for restructuring students’ existing knowledge into a more conceptual and systematic framework. Moreover, barriers to learning were identified as well. For example, conceptual interferences between the concepts of space/place and other geographical key concepts such as sustainability were observed, partially impeding the formation of a clear conceptual framework in students’ minds. The results provide insights for teaching implications, such as emphasising a single key concept to avoid conceptual interferences, and suggest further research avenues, including longitudinal studies to empirically derive learning progressions related to space and place.
An Outphasing Architecture Based on Parallel Radio Frequency–Pulse Width Modulation Method for All-Digital Transmitter
Authors: Wang Xu , Zhou Qiang , Wang Min , Fu Haoyang
Published: January 2024

For the existing outphasing architectures of an all-digital transmitter (ADTx), the required sampling rate of the signal is too high, which increases the difficulty of digital radio frequency pulse width modulation (RF-PWM) processing. In this paper, we present an outphasing architecture based on the parallel RF-PWM method for an ADTx. Through polyphase interpolation, two baseband outphasing signals are divided into multiple low-rate signals to process simultaneously. The parallel outphasing signals are modulated and encoded to obtain 1-bit parallel signals, which are, respectively, transmitted to multigigabit transceivers (MGTs) to generate two two-level high-speed pulses with different phases. Finally, a three-level high-speed pulse is synthesized and amplified through the switching power amplifier. Through this parallel scheme, the sampling rate of digital RF-PWM signal processing is effectively reduced. Moreover, to explore a pulse encoding method, the outphasing architecture is combined with a zero-crossing comparison through an angle calculation and quadrant judgment, which simplifies the modulation and encoding process. In addition, the impact of the sub-filter order and the number of parallel paths on system performance is analyzed. The simulation results show that for a 16QAM signal with a baseband bandwidth of 20 MHz and a carrier frequency of 200 MHz, the adjacent channel power ratio (ACPR) is below −45 dBc and the error vector magnitude (EVM) is below 1% in the proposed scheme.
A Review of Immersive Technologies, Knowledge Representation, and AI for Human-Centered Digital Experiences
Authors: Partarakis Nikolaos , Zabulis Xenophon
Published: January 2024

The evolution of digital technologies has resulted in the emergence of diverse interaction technologies. In this paper, we conducted a review of seven domains under a human-centric approach user interface design, human-centered web-based information systems, semantic knowledge representation, X-reality applications, human motion and 3D digitization, serious games, and AI. In this review, we studied these domains concerning their impact on the way we interact with digital interfaces, process information, and engage in immersive experiences. As such, we highlighted the shifts in design paradigms, user-centered principles, and the rise of web-based information systems. The results of such shifts are materialized in modern immersive technologies, semantic knowledge representation, serious games, and the facilitation of artificial intelligence for interactions. Through this exploration, we aimed to assist our understanding of the challenges that lie ahead. The seamless integration of technologies, ethical considerations, accessibility, education for technological literacy, interoperability, user trust, environmental sustainability, and regulatory frameworks are becoming significant. These challenges present opportunities for the future to enrich human experiences while addressing societal needs. This paper lays the groundwork for thoughtful and innovative approaches to the challenges that will define the future of human–computer interaction and information technologies.
Instance Segmentation of Underwater Images by Using Deep Learning
Authors: Chen Jianfeng , Zhu Shidong , Luo Weilin
Published: January 2024

Based on deep learning, an underwater image instance segmentation method is proposed. Firstly, in view of the scarcity of underwater related data sets, the size of the data set is expanded by measures including image rotation and flipping, and image generation by a generative adversarial network (GAN). Next, the underwater image data set is finally constructed by manual labeling. Then, in order to solve the problems of color shift, blur and the poor contrast of optical images caused by the complex underwater environment and the attenuation and scattering of light, an underwater image enhancement algorithm is used to first preprocess the data set, and several algorithms are discussed, including multi-scale Retinex (MSRCR) with color recovery, integrated color model (ICM), relative global histogram stretching (RGHS) and unsupervised color correction (UCM), as well as the color shift removal proposed in this work. Specifically, the results indicate that the proposed method can largely increase the segmentation mAP (mean average precision) by 85.7% compared with without the pretreatment method. In addition, based on the characteristics of the constructed underwater dataset, the feature pyramid network (FPN) is improved to some extent, and the preprocessing method is further combined with the improved network for experiments and compared with other neural networks to verify the effectiveness of the proposed method, thus achieving the effect and purpose of improving underwater image instance segmentation and target recognition. The experimental analysis results show that the proposed model can achieve a mAP of 0.245, which is about 1.1 times higher than other target recognition models.
Energy Recovery from Municipal Solid Waste through Co-Gasification Using Steam or Carbon Dioxide with Olive By-Products
Authors: Vamvuka Despina , Tsilivakos Petros
Published: January 2024

The valorization of untreated municipal waste (MSW) biochar for energetic uses, through its co-gasification with olive stone (OST) biochar under a steam or carbon dioxide atmosphere, was investigated. The experiments were conducted in a fixed bed unit and a thermal analysis–mass spectrometer system. The thermal behavior, reactivity, conversion, product gas composition, syngas yield and energy potential were determined, while the influence of the fuel’s internal structure, chemical functional groups and operating conditions were examined. The concentrations of H2 and CO2 in the product gas mixture under a steam atmosphere were increased with steam/biochar ratio, while that of CO was reduced. At a steam/biochar = 3 H2 yield, the higher heating value and conversion for the OST were 52.8%, 10.8 MJ/m3 and 87.5%; for the MSW, they were 44.4%, 9.9 MJ/m3 and 51.5%, whereas for their blend, they were 50%, 10.6 MJ/m3 and 76.6%, respectively. Under a carbon dioxide atmosphere, the reactivity and conversion of the OST biochar (84%) were significantly higher as compared with the MSW biochar (50%). The higher heating value of the product gas was 12.4–12.9 MJ/m3. Co-gasification of the MSW with OST (in proportions 30:70) resulted in the enhanced reactivity, conversion, syngas yield and heating value of product gas compared with gasification of solely MSW material.
Safety of Chinese Cabbage Waste and Rice Straw Mixed Silage and Its Effect on Growth and Health Performance of Hu Sheep
Authors: Lu Zhiqi , Li Chuang , Hou Lie , Zhang Zhenbin , Lu Yue , Xue Chun , Qi Ruxin , Zafar Muhammad Hammad , Xu Jun , Wang Mengzhi
Published: January 2024

Improper disposal of vegetable waste can cause serious environmental pollution, but because they contain huge water content and organic matter, they are not suitable for disposal by methods such as incineration and landfill. However, vegetable waste contains a large amount of nutrients and have some complementary effects with rice straw in terms of physical structure, nutrients, and moisture. In this experiment, the plant feed (corn husk, peanut shells and sorghum shells) was used as the control group (CON group), and the mixed silage of Chinese cabbage waste and rice straw (mixed silage) was used as the experiment group (TRE group), and its safety performance was evaluated by testing its toxin content, pesticide residues, vitamin contents and feeding experiment of Hu sheep. In the animal experiment, 16 healthy Hu sheep (5.5 months, 39.11 ± 4.16 kg) were randomly divided into two groups of 8 each. The results of the safety performance evaluation showed that the content of mycotoxins, heavy metals, and nitrites as well as pesticide residues in the crude feeds of both groups were within the range of Chinese feed hygiene standards. In addition, the levels of deoxynivalenol (DON) and aflatoxin (AFT) in the CON group were lower, while the content of ochratoxin (OTA) and zearalenone were higher than those in the TRE group (p < 0.05). The levels of plumbum(Pb), chromium (Cr), cadmium (Cd), and nitrite in the CON group were lower than the mixed silage, while the levels of As were higher than the mixed silage (p < 0.05). It is worth noticing that the content of vitamin B2 (VB2) and vitamin C (VC) in the TRE group was higher than the CON group (p < 0.05). The results of the feeding experiment showed that the mixed silage did not affect the growth performance, nutrient digestibility, organ index, and intestinal index of Hu sheep (p > 0.05). In addition, the mixed silage reduced the weight of omasum, the proportion of omasum to live weight before slaughter, the amount of compound stomach, and the proportion of compound stomach to live weight before slaughter, which were higher than those in the TRE group (p < 0.05). The thickness of the basal layer of the rumen abdominal sac, the red blood cell count, the content of IL-10, and TNF-α in the blood, and TNF-α content in the rumen of the Hu sheep in the TRE group were higher than the CON group (p < 0.05). In conclusion, the feed safety index content of the mixed silage did not exceed the Chinese feed hygiene and safety standards and did not cause adverse effects on the growth performance of the Hu sheep, and it improved the immune performance of the body and digestive tract of the sheep to a certain extent and promoted the healthy development of the sheep.
A GPU-Accelerated Modern Fortran Version of the ECHO Code for Relativistic Magnetohydrodynamics
Authors: Del Zanna Luca , Landi Simone , Serafini Lorenzo , Bugli Matteo , Papini Emanuele
Published: January 2024

The numerical study of relativistic magnetohydrodynamics (MHD) plays a crucial role in high-energy astrophysics but unfortunately is computationally demanding, given the complex physics involved (high Lorentz factor flows, extreme magnetization, and curved spacetimes near compact objects) and the large variety of spatial scales needed to resolve turbulent motions. A great benefit comes from the porting of existing codes running on standard processors to GPU-based platforms. However, this usually requires a drastic rewriting of the original code, the use of specific languages like CUDA, and a complex analysis of data management and optimization of parallel processes. Here, we describe the porting of the ECHO code for special and general relativistic MHD to accelerated devices, simply based on native Fortran language built-in constructs, especially do concurrent loops, few OpenACC directives, and straightforward data management provided by the Unified Memory option of NVIDIA compilers. Thanks to these very minor modifications to the original code, the new version of ECHO runs at least 16 times faster on GPU platforms as compared to CPU-based ones. The chosen benchmark is the 3D propagation of a relativistic MHD Alfvén wave, for which strong and weak scaling tests performed on the LEONARDO pre-exascale supercomputer at CINECA are provided (using up to 256 nodes corresponding to 1024 GPUs, and over 14 billion cells). Finally, an example of high-resolution relativistic MHD Alfvénic turbulence simulation is shown, demonstrating the potential for astrophysical plasmas of the new GPU-based version of ECHO.
Crop Improvement: Comparison of Transgenesis and Gene Editing
Authors: Permyakova Natalya V. , Deineko Elena V.
Published: January 2024

The development and improvement of molecular biology methods have led to the creation of new technologies that make it possible to modify plant genomes by transferring and integrating into the genomes’ heterologous genes from various expression systems (genetic engineering), as well as inducing knockouts of one or more target genes of interest (genomic editing). The development of genome-editing methods is a new milestone in the development of modern breeding methods and certainly relies on the knowledge and technologies developed for transgenesis. This review will discuss issues related to the advantages and disadvantages of both technologies for improving the economically valuable traits of important crops.
Production of Phenylpropanoids, Naphthodianthrones and Antioxidant Status of Hypericum perforatum L. Transgenic Shoots
Authors: Tusevski Oliver , Todorovska Marija , Todorovska Ivana , Petreska Stanoeva Jasmina , Gadzovska Simic Sonja
Published: January 2024

Fifteen transgenic shoot clones (TS A-TS O) regenerated from Hypericum perforatum hairy roots were evaluated for growth characteristics, phenylpropanoid, and naphthodianthrone production, as well as non-enzymatic and enzymatic antioxidant status. Transgenic shoots displayed higher biomass production and non-altered phenotypes compared to control shoots. Phenylpropanoid production in TS was not related to phenylalanine ammonia lyase activity indicating that alternative enzymes could be involved in the regulation of phenylpropanoid/flavonoid biosynthesis. The positive correlation between phenylpropanoids (phenolic acids, flavonoids, flavan-3-ols, tannins) and non-enzymatic antioxidant assays suggested that these compounds are the main contributors to the redox state and radical scavenging properties of TS clones. More importantly, enhanced naphthodianthrone production (hypericin, pseudohypericin and protopseudohypericin) in TS was associated with the number of leaf dark glands. Concerning the enzymatic antioxidant status of TS, guaiacol peroxidase was shown as the main enzyme that turns down H2O2 levels, while the upregulation of superoxide dismutase coincided with decreased O2•− production. The efficiency of antioxidant enzymes for O2•− and H2O2 neutralization decreased the level of MDA and consequently delayed lipid peroxidation in TS clones. Altogether, H. perforatum transgenic shoots could be proposed as a prospective biotechnological system for scale-up production of naphthodianthrones and phenylpropanoids with antioxidant properties.
Whole-Transcriptome Sequencing Reveals That mRNA and ncRNA Levels Correlate with Pleurotus cornucopiae Color Formation
Authors: Zhang Yingjie , Huang Chenyang , Zhao Mengran , Li Guangyu , Wu Zeyin , Zhang Jie , Zhang Lijiao
Published: January 2024

Pleurotus cornucopiae cap color is an important commercial trait. The roles of non-coding RNA molecules (ncRNAs) in fungal fruiting body color formation are unclear. Whole-transcriptome analyses were performed, identifying messenger RNA (mRNA) and ncRNA (including long stranded non-coding RNA (lncRNA), micro RNA-like (milRNA), and circular RNA (circRNA)) molecules in three P. cornucopiae strains with different cap colors (grayish-black, grayish-white, and white), to investigate their roles in cap color formation. Differentially expressed mRNAs (n = 3604), lncRNAs (n = 520), milRNAs (n = 56), and circRNAs (n = 20) were identified among the three different color strains. Differentially expressed mRNAs and ncRNAs target genes were mainly involved in tyrosine, arachidonic acid, and histidine metabolism, plus other pathways potentially important in color formation. A competitive endogenous RNA (ceRNA) network and three mRNA and ncRNA co-expression networks were constructed, revealing relationships contributing to cap color formation, involving genes with roles in protein phosphorylation, and phenylalanine, tyrosine, and tryptophan biosynthesis. qRT-PCR analysis confirmed that mRNA and ncRNA levels were related to P. cornucopiae color. These regulatory networks may be crucial in P. cornucopiae color formation. Our data provide a new perspective to support further study of the mechanisms underlying P. cornucopiae color formation.
Multivariate Analysis and Optimization of the Relationship between Soil Nutrients and Berry Quality of Vitis vinifera cv. Cabernet Franc Vineyards in the Eastern Foothills of the Helan Mountains, China
Authors: Li Yashan , Xiao Jinnan , Yan Yinfang , Liu Weiqiang , Cui Ping , Xu Chengdong , Nan Lijun , Liu Xu
Published: January 2024

The aim of this study is to explore the relationship between soil nutrients and berry quality for the wine grape Vitis vinifera cv. Cabernet Franc in the eastern foothills of the Helan Mountains, and subsequently to optimize soil nutrient conditions for optimal berry quality, thus providing guidance for vineyard soil management. Based on the basic data on soil nutrients and berry quality indicators, a partial least squares regression method was used to screen for major soil nutrient factors affecting the grape quality index. Then, the selected soil nutrient factors were taken as independent variables and the corresponding grape quality indicators were taken as dependent variables and a multilinear regression equation was formulated by the method of multivariate linear regression. Finally, the optimal solution for fruit quality and soil nutrients was solved using linear programming equations. The results showed that there was a lack of total nitrogen, organic matter, nitrate nitrogen, ammonium nitrogen, and available phosphorus in the soil nutrients, and an alkaline soil. There is a significant positive correlation between some soil nutrient indices, and there is also a multivariate linearity problem. Among all berry quality indices, titratable acid, tannin, and anthocyanin were negatively correlated with eleven and ten soil indices, respectively, while other berry quality indices were positively correlated with most soil nutrient indices. The optimal parameters for grape quality were determined using the method of linear programming equations, and the corresponding soil nutrient indicators content were defined.
Assessing the Impact of Non-Pharmaceutical Interventions on Consumer Mobility Patterns and COVID-19 Transmission in the US
Authors: Zuccarelli Joseph , Seaman Laura , Rader Kevin
Published: January 2024

The initial outbreak of COVID-19 during late December 2019 and the subsequent global pandemic markedly changed consumer mobility patterns worldwide, largely in response to government-ordered non-pharmaceutical interventions (NPIs). In this study, we investigate these changes as they relate to the initial spread of COVID-19 within two states—Massachusetts and Michigan. Specifically, we use linear and generalized linear mixed-effects models to quantify the relationship between four NPIs and individuals’ point-of-sale (POS) credit card transactions, as well as the relationship between subsequent changes in POS transactions and county-level COVID-19 case growth rates. Our analysis reveals a significant negative association between NPIs and daily POS transactions, particularly a dose–response relationship, in which stringent workplace closures, stay-at-home requirements, and gathering restrictions were all associated with decreased POS transactions. We also uncover a significant positive association between 12-day lagged changes in POS transactions compared to pre-pandemic baselines and county-level COVID-19 case growth rates. Overall, our study supports previous findings that early NPIs reduced human mobility and COVID-19 transmission in the US, providing policymakers with quantitative evidence concerning the effectiveness of NPIs.
Local Habitat Complexity and Its Effects on Herbivores and Predators in Urban Agroecosystems
Authors: Lucatero Azucena , Jha Shalene , Philpott Stacy M.
Published: January 2024

In urban community gardens, cultivated vegetation provides variable levels of habitat complexity, which can suppress pests by promoting predator diversity and improving pest control. In this study, we examine three components of the structural complexity of garden vegetation (cover, diversity, and connectivity) to investigate whether higher garden vegetation complexity leads to fewer herbivores, more predators, and higher predation. We worked in eight community gardens where we quantified vegetation complexity, sampled the arthropod community, and measured predation on corn earworm eggs. We found that plots with high vegetation cover supported higher species richness and greater abundance of predatory insects. High vegetation cover also supported a greater abundance and species richness of spiders. In contrast, high vegetation diversity was negatively associated with predator abundance. While high predator abundance was positively associated with egg predation, greater predator species richness had a negative impact on egg predation, suggesting that antagonism between predators may limit biological control. Community gardeners may thus manipulate vegetation cover and diversity to promote higher predator abundance and diversity in their plots. However, the species composition of predators and the prevalence of interspecific antagonism may ultimately determine subsequent impacts on biological pest control.
Antideuteron Identification in Space with Helium Calorimeter
Authors: Nozzoli Francesco , Rashevskaya Irina , Ricci Leonardo , Rossi Francesco , Spinnato Piero , Verroi Enrico , Zuccon Paolo , Giovanazzi Gregorio
Published: January 2024

The search for low-energy antideuterons in cosmic rays allows the addressing of fundamental physics problems testing for the presence of primordial antimatter and the nature of Dark Matter. The PHeSCAMI (Pressurized Helium Scintillating Calorimeter for AntiMatter Identification) project aims to exploit the long-living metastable states of the helium target for the identification of low-energy antideuterons in cosmic rays. A space-based pressurized helium calorimeter would provide a characteristic identification signature based on the coincident detection of a prompt scintillation signal emitted by the antideuteron energy loss during the slowing-down phase in the gas, and the (≈µs) delayed scintillation signal provided by the charged pions produced in the subsequent annihilation. The performance of a high-pressure (200-bar) helium scintillator prototype, tested in the INFN-TIFPA laboratory, will be summarized.
Tattoo-Associated Skin Reaction in a Melanoma Patient Receiving B-RAF and MEK Inhibitors: A Case Report with an Emphasis on Etiopathogenic and Histological Features
Authors: Baratta Silvia , Cazzato Gerardo , Foti Caterina , Ingravallo Giuseppe , Lospalluti Lucia , Laface Carmelo , Filotico Raffaele , Ambrogio Francesca
Published: January 2024

Tattoo-associated cutaneous reactions have become quite frequent given the increasing percentage of tattooed subjects globally and also in Italy. On the other hand, the increasing use of target therapy is showing the ability of these drugs to affect the immune system and also cause adverse tattoo-related reactions. In this paper, we report a case of a 42-year-old patient with stage-IIID melanoma undergoing treatment with Dabrafenib and Trametinib. The patient reported erythema, oedema and scaling in areas of the body containing a black tattoo, and, conversely, no signs and/or symptoms in areas with tattoos of a different color. Histopathological and immunohistochemical features indicated a lympho-histiocytic reaction with a granulomatous morphology, mainly distributed around the vessels and hair adnexa. By discussing the cases reported in the literature prior to ours, we concluded and provided the possible indications of the pathogenesis.
Potentially Inappropriate Prescribing and Potential Prescribing Omissions and Their Association with Adverse Drug Reaction-Related Hospital Admissions
Authors: Brannigan Ross , Hughes John E. , Moriarty Frank , Wallace Emma , Kirke Ciara , Williams David , Bennett Kathleen , Cahir Caitriona
Published: January 2024

Background: This study aimed to determine the prevalence of potentially inappropriate prescribing (PIP) and potential prescribing omissions (PPOs) and their association with ADR-related hospital admissions in patients aged ≥ 65 years admitted acutely to the hospital. Methods: Information on medications and morbidities was extracted from the Adverse Drug Reactions in an Ageing Population (ADAPT) cohort (N = 798: N = 361 ADR-related admissions; 437 non-ADR-related admissions). PIP and PPOs were assessed using Beers Criteria 2019 and STOPP/START version 2. Multivariable logistic regression (adjusted odds ratios (aOR), 95%CI) was used to examine the association between PIP, PPOs and ADR-related admissions, adjusting for covariates (age, gender, comorbidity, polypharmacy). Results: In total, 715 (90%; 95% CI 87–92%) patients had ≥1 Beers Criteria, 555 (70%; 95% CI 66–73%) had ≥ 1 STOPP criteria and 666 patients (83%; 95% CI 81–86%) had ≥ 1 START criteria. Being prescribed at least one Beers (aOR = 1.66, 95% CI = 1.00–2.77), or meeting STOPP (aOR = 1.07, 95% CI = 0.79–1.45) or START (aOR = 0.72; 95%CI = 0.50–1.06) criteria or the number of PIP/PPO criteria met was not significantly associated with ADR-related admissions. Patients prescribed certain drug classes (e.g., antiplatelet agents, diuretics) per individual PIP criteria were more likely to have an ADR-related admission. Conclusion: There was a high prevalence of PIP and PPOs in this cohort but no association with ADR-related admissions.
The Influence of Mobility Training on the Myofascial Structures of the Back and Extremities
Authors: Slomka Gunda , Schleip Robert , Frei Peter , Kurpiers Nicolas , Krämer Michael , Bauermeister Katharina , Bauermeister Wolfgang
Published: January 2024

Background: The subject of the study was the effect of a multicomponent program (Mobility Routine) on muscular and fascial stiffness, flexibility, subjective well-being, and body perception. Methods: The assumption was that high physical stress affects myofascial structures and joint range of motion. The assessment of myofascial stiffness employed a Shear Wave Elastography. The joint flexibility, pressure pain threshold, and subjective experiences with regard to tension, pain, and general discomfort were documented. Results: In the CT group, a greater increase in stiffness was measured in fewer measurement areas compared to the MR group. MR demonstrated superior gains in flexibility compared to CT. Both groups experienced significant reductions in pain, tension, and discomfort. In conclusion, repetitive motion patterns akin to CT lead to increased myofascial stiffness, whereas MR yields more balanced stiffness development, compensates for asymmetries, and improves body awareness. Conclusions: Hence, this study highlights the advantages of mobility training over Crosstrainer exercises and provides valuable insights for the recommendation of training regimens aiming at the enhancement of musculoskeletal functionality and overall well-being.