bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual RNA-seq in filarial nematodes and Wolbachia endosymbionts using RNase H based ribosomal RNA depletion
Authors: Cantin Lindsey J. , Gregory Vanessa , Blum Laura N. , Foster Jeremy M.
Published: May 2024

Lymphatic filariasis is caused by parasitic nematodes and is a leading cause of disability worldwide. Many filarial worms contain the bacterium Wolbachia as an obligate endosymbiont. RNA sequencing is a common technique used to study their molecular relationships and to identify potential drug targets against the nematode and bacteria. Ribosomal RNA (rRNA) is the most abundant RNA species, accounting for 80–90% of the RNA in a sample. To reduce sequencing costs, it is necessary to remove ribosomal reads through poly-A enrichment or ribosomal depletion. Bacterial RNA does not contain a poly-A tail, making it difficult to sequence both the nematode and Wolbachia from the same library preparation using standard poly-A selection. Ribosomal depletion can utilize species-specific oligonucleotide probes to remove rRNA through pull-down or degradation methods. While species-specific probes are commercially available for many commonly studied model organisms, there are currently limited depletion options for filarial parasites. Here, we performed total RNA sequencing from Brugia malayi containing the Wolbachia symbiont (wBm) and designed ssDNA depletion probes against their rRNA sequences. We compared the total RNA library to poly-A enriched, Terminator 5′-Phosphate-Dependent Exonuclease treated, NEBNext Human/Bacteria rRNA depleted and our custom nematode probe depleted libraries. The custom nematode depletion library had the lowest percentage of ribosomal reads across all methods, with a 300-fold decrease in rRNA when compared to the total RNA library. The nematode depletion libraries also contained the highest percentage of Wolbachia mRNA reads, resulting in a 16–1,000-fold increase in bacterial reads compared to the other enrichment and depletion methods. Finally, we found that the Brugia malayi depletion probes can remove rRNA from the filarial worm Dirofilaria immitis and the majority of rRNA from the more distantly related free living nematode Caenorhabditis elegans. These custom filarial probes will allow for future dual RNA-seq experiments between nematodes and their bacterial symbionts from a single sequencing library.
Remote intelligent perception system for multi-object detection
Authors: Alazeb Abdulwahab , Chughtai Bisma Riaz , Al Mudawi Naif , AlQahtani Yahya , Alonazi Mohammed , Aljuaid Hanan , Jalal Ahmad , Liu Hui
Published: May 2024

Introduction

During the last few years, a heightened interest has been shown in classifying scene images depicting diverse robotic environments. The surge in interest can be attributed to significant improvements in visual sensor technology, which has enhanced image analysis capabilities.

Methods

Advances in vision technology have a major impact on the areas of multiple object detection and scene understanding. These tasks are an integral part of a variety of technologies, including integrating scenes in augmented reality, facilitating robot navigation, enabling autonomous driving systems, and improving applications in tourist information. Despite significant strides in visual interpretation, numerous challenges persist, encompassing semantic understanding, occlusion, orientation, insufficient availability of labeled data, uneven illumination including shadows and lighting, variation in direction, and object size and changing background. To overcome these challenges, we proposed an innovative scene recognition framework, which proved to be highly effective and yielded remarkable results. First, we perform preprocessing using kernel convolution on scene data. Second, we perform semantic segmentation using UNet segmentation. Then, we extract features from these segmented data using discrete wavelet transform (DWT), Sobel and Laplacian, and textual (local binary pattern analysis). To recognize the object, we have used deep belief network and then find the object-to-object relation. Finally, AlexNet is used to assign the relevant labels to the scene based on recognized objects in the image.

Results

The performance of the proposed system was validated using three standard datasets: PASCALVOC-12, Cityscapes, and Caltech 101. The accuracy attained on the PASCALVOC-12 dataset exceeds 96% while achieving a rate of 95.90% on the Cityscapes dataset.

Discussion

Furthermore, the model demonstrates a commendable accuracy of 92.2% on the Caltech 101 dataset. This model showcases noteworthy advancements beyond the capabilities of current models.
Complexity synchronization in living matter: a mini review
Authors: West Bruce J.
Published: May 2024

Fractal time series have been argued to be ubiquitous in human physiology and some of the implications of that ubiquity are quite remarkable. One consequence of the omnipresent fractality is complexity synchronization (CS) observed in the interactions among simultaneously recorded physiologic time series discussed herein. This new kind of synchronization has been revealed in the interaction triad of organ-networks (ONs) consisting of the mutually interacting time series generated by the brain (electroencephalograms, EEGs), heart (electrocardiograms, ECGs), and lungs (Respiration). The scaled time series from each member of the triad look nothing like one another and yet they bear a deeply recorded synchronization invisible to the naked eye. The theory of scaling statistics is used to explain the source of the CS observed in the information exchange among these multifractal time series. The multifractal dimension (MFD) of each time series is a measure of the time-dependent complexity of that time series, and it is the matching of the MFD time series that provides the synchronization referred to as CS. The CS is one manifestation of the hypothesis given by a “Law of Multifractal Dimension Synchronization” (LMFDS) which is supported by data. Therefore, the review aspects of this paper are chosen to make the extended range of the LMFDS hypothesis sufficiently reasonable to warrant further empirical testing.
Rupture point is associated with divergent hemodynamics in intracranial aneurysms
Authors: Hejčl Aleš , Brunátová Jana , Švihlová Helena , Víteček Jan , Wünschová Andrea Vítečková , Sejkorová Alena , Stratilová Mária Hundža , Radovnický Tomáš , Sameš Martin , Hron Jaroslav
Published: May 2024

Background

Understanding the risk factors leading to intracranial aneurysm (IA) rupture have still not been fully clarified. They are vital for proper medical guidance of patients harboring unruptured IAs. Clarifying the hemodynamics associated with the point of rupture could help could provide useful information about some of the risk factors. Thus far, few studies have studied this issue with often diverging conclusions.

Methods

We identified a point of rupture in patients operated for an IAs during surgery, using a combination of preoperative computed tomography (CT) and computed tomography angiography (CTA). Hemodynamic parameters were calculated both for the aneurysm sac as a whole and the point of rupture. In two cases, the results of CFD were compared with those of the experiment using particle image velocimetry (PIV).

Results

We were able to identify 6 aneurysms with a well-demarcated point of rupture. In four aneurysms, the rupture point was near the vortex with low wall shear stress (WSS) and high oscillatory shear index (OSI). In one case, the rupture point was in the flow jet with high WSS. In the last case, the rupture point was in the significant bleb and no specific hemodynamic parameters were found. The CFD results were verified in the PIV part of the study.

Conclusion

Our study shows that different hemodynamic scenarios are associated with the site of IA rupture. The numerical simulations were confirmed by laboratory models. This study further supports the hypothesis that various pathological pathways may lead to aneurysm wall damage resulting in its rupture.
A case report of neonatal incontinentia pigmenti complicated by severe cerebrovascular lesions in one of the male monozygotic twins
Authors: Lin Xiaofeng , Zhang Wei , Zhou Ping
Published: May 2024

Background

This article reports a case of neonatal incontinentia pigmenti onset in only one male monozygotic twin with characteristic skin lesions after birth followed by severe cerebrovascular lesions.

Case presentation

A male infant, the first of monozygotic twins, was born with multiple yellow pustules all over his body, repeated new herpes at different sites during the course of the disease, aggravated by fusion, warty crusts, and hyperpigmentation; biopsy pathology suggested eosinophilic spongiform edema of the skin. Peripheral blood eosinophils were significantly elevated, and brain magnetic resonance imaging revealed diffuse multiple cystic and lamellar abnormal signal areas in the left frontal and parietal lobes. On day 30, the infant showed neurological symptoms, such as poor response and apnea, and an emergency cranial computed tomography scan revealed abnormal changes in the left cerebral hemisphere and bilateral cerebellum. After admission, he was given a potassium permanganate bath and topical mupirocin for 1 month, and the skin abnormalities improved. He was treated with mechanical ventilation and vasoactive drugs for 2 days after the cerebrovascular accident, and died the same day after the parents chose hospice care. No deletion variants or point mutations were detected in subsequent genetic tests, and chromosomal copy number variation tests revealed different degrees of chimeric duplications and deletions in different regions of chromosomes Y and 3. The parents were healthy, and his twin brother had normal growth and development with no abnormalities at multiple follow-up visits.

Conclusion

Neonatal incontinentia pigmenti in only one male monozygotic twin is extremely rare and the genetic diagnosis is challenging. Awareness of the combined cerebrovascular lesions needs to be enhanced, and potential prevention and treatment methods need to be explored to improve the prognosis.
Fibromyalgia in obstructive sleep apnea-hypopnea syndrome: a systematic review and meta-analysis
Authors: He Jie , Chen Meifeng , Huang Na , Wang Bo
Published: May 2024

Introduction: Fibromyalgia (FM) is a common condition in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS). This meta-analysis aimed to evaluate differences in sleep monitoring indicators between patients with OSAHS and positive FM and patients with OSAHS and negative FM and to determine the incidence of FM in patients with OSAHS.

Methods: An exhaustive literature review was conducted to analyze the incidence of FM in patients with OSAHS, using online databases, including PubMed, EMBASE, Web of Science, CNKI, and Wanfang, both in English and Chinese. The quality of the included studies was assessed by two researchers using the Newcastle−Ottawa Scale scores. The acquired data were analyzed using Stata 11.0 software. Continuous variables were combined and analyzed using the weighted mean difference as the effect size. Conjoint analyses were performed using random-effects (I2 > 50%) or fixed-effect (I2 ≤ 50%) models based on I2 values.

Results: Fourteen studies met the inclusion criteria. This study showed that 21% of patients with OSAHS experienced FM. Subgroup analyses were performed based on race, age, sex, body mass index, and diagnostic criteria for patients with OSAHS. These findings indicate that obese patients with OSAHS have a higher risk of FM, similar to females with OSAHS. Regarding most sleep monitoring indicators, there were no discernible differences between patients with OSAHS with positive FM and those with negative FM. However, patients with positive FM had marginally lower minimum arterial oxygen saturation levels than those with negative FM. The current literature suggests that patients with OSAHS have a high incidence of FM (21%), and FM has little effect on polysomnographic indicators of OSAHS.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024510786, identifier CRD42024510786
Effects of nicotinamide riboside in ovo feeding on high-yield broiler performance, meat quality, and myopathy incidence
Authors: Maynard Clay J. , Gonzalez John M. , Haginouchi Taketo , Ellis Olivia G. , Jackson Ashunti R. , Owens Casey M.
Published: May 2024

Introduction: The objective of this study was to examine the effects of in ovo nicotinamide riboside (NR) feeding on high-yield broiler growth and meat quality.

Methods: Fertilized Cobb 700 by-product eggs (N = 3,240) were randomly assigned to one of four in ovo treatments and injected with 0 (0NR), 250 (250NR), 500 (500NR), or 1,000 (1,000NR) mM NR at incubation-day 10. Chicks were hatched, vent sexed, and randomly placed 18 per pen in one of 32 floor pens. On day 48, birds were processed and deboned.

Results: There were dose effects for all part weights (p < 0.05). Pectoralis major weight of 250, 500, and 1,000NR carcasses were heavier than 0NR (p < 0.03) but did not differ from remaining NR doses (p > 0.26). Pectoralis minor weight of 250NR carcasses was greater (p < 0.01) than 0NR and did not differ from other NR tenders (p > 0.21). Pectoralis minor weight of 500 and 1,000NR carcasses was greater than 0NR (p < 0.09), but did not differ (P = 0.82) from each other. There were no dose effects for all Pectoralis major and minor myopathy scores and incidence except incidence of tenders scoring “0” and “1” for woody-like tender. Percentage of NR1,000 tenders scoring 0 and 1 for woody-like tender were less than and greater than all other treatments, respectively (p < 0.05). There were no differences among remaining NR doses and NR0 tenders (p > 0.10). There were dose effects for muscle fiber number (P = 0.03). There tended to be more muscle fibers within 250 and 1,000NR muscles compared to 0NR (p < 0.09). Pectoralis major muscle from 500NR did not differ in muscle fiber number compared to 250 and 1,000NR (p > 0.18), but had more (p < 0.01) fibers than 0NR muscle. There tended to be more fibers in 250 and 1,000NR muscles compared to 0NR muscle (p < 0.09).

Discussion: Nicotinamide riboside in ovo feeding caused birds to produce heavier parts; however, myopathy scores and incidence were minimally affected which may have been due greater muscle fiber number.
The effect of a Schema-based method on correcting persistent errors in mental arithmetic: an experimental study
Authors: Chen Shufang , Liu Dawei , Yan Huifen , Ma Yong
Published: May 2024

Introduction

Arithmetic calculation is a fundamental skill for mathematical learning and daily life. However, elementary school students often make errors in practice.

Methods

Grounded in the schema theory and the memory retrieval theory of mental arithmetic, this study employs a controlled experiment to investigate the effect of a schema-based method in correcting persistent errors in mental arithmetic, specifically in the context of simple addition operations. The experimental group utilizes a schema-based method to help participants rectify incorrect answers in memory retrieval, while the control group did not receive this treatment.

Results

The results showed that significant differences emerged between the experimental and control groups in both the post-test performance and the reduction of persistent error count, indicating that the experimental group had rectified incorrect answers in memory; and persistent errors in simple addition were indeed caused by interference from incorrect answers during memory retrieval; and the schema-based method proves to be effective.

Discussion

The findings of this study contribute to enhancing practical mental arithmetic instruction, assisting students in correcting relevant errors, and improving their mental arithmetic abilities. Not only does it offer directive guidance for teaching practices, but it also provides an enlightening reference for promoting innovative teaching methods.
Exploring consumer responses to official endorsement: roles of credibility and attractiveness attributes in live streaming
Authors: Cheng Guo , Li Wenjie , He Mingli , Liao Luyuan
Published: May 2024

Introduction

Official endorsement, distinct from celebrity, expertise, and peer endorsement, introduces a new paradigm where local government officials use online platforms, particularly live streaming, to promote local products and brands.

Methods

This study examines the influence of official endorsement on consumer responses using the source credibility and source attractiveness models. We developed a framework that considers official credibility and attractiveness attributes as antecedents, and consumer perceived security and enjoyment as mediators, affecting purchase intention and local brand awareness. The study also incorporates variables such as consumer region and power distance belief.

Results

Data from 594 responses obtained through an online survey were analyzed using structural equation modeling. The results indicate that official credibility attributes (expertise, trustworthiness, government credibility) enhances consumer perceived security, while official attractiveness attributes (physical attractiveness, interaction friendliness, and similarity with consumers) increases consumer enjoyment. Both perceived security and enjoyment positively influence purchase intention and local brand awareness. These relationships are partially moderated by consumer region and power distance belief.

Discussion

This research pioneers the study of official endorsements, expanding the endorsement literature. It also provides practical insights for marketing professionals and government officials on leveraging official endorsements to enhance the value of local products and brands..
Intellectual disability and autism prevalence in Western Australia: impact of the NDIS
Authors: Bourke Jenny , Sanders Richard , Jones Jocelyn , Ranjan Maathumai , Wong Kingsley , Leonard Helen
Published: May 2024

Introduction

Estimates of the prevalence of intellectual disability or autism spectrum disorder (ASD) may vary depending on the methodology, geographical location, and sources of ascertainment. The National Disability Insurance Scheme (NDIS) in Australia was introduced progressively from 2016 to provide individualized funding for eligible people with a significant and permanent disability.

Methods

Its recent inclusion as a source of ascertainment in the population-based Intellectual Disability Exploring Answers (IDEA) database in Western Australia has allowed comparisons of the prevalence of intellectual disability and ASD before and after its introduction.

Results

Prevalence of intellectual disability in 2020 was 22.5 per 1,000 (/1,000) live births compared with previous estimates in 2010 of 17/1,000, and for ASD, the estimate was 20.7/1,000 in 2020 compared with 5.1 /1,000 in 2010. Whilst the prevalence of ASD in Aboriginal individuals was about two-thirds that of non-Aboriginals, there was an increased prevalence of ASD in Aboriginal children under 10 years compared with non-Aboriginal children.

Discussion

The concurrent relaxation of ASD diagnostic practice standards in Western Australia associated with the administration of access to the NDIS and the release of the National Guidelines empowering single diagnosticians to determine the appropriateness of engaging additional diagnosticians to form a multidisciplinary team on ASD diagnosis, appear to be important factors associated with the increase in ASD diagnoses both with and without intellectual disability.
Social activities in multidomain dementia prevention interventions: insights from practice and a blueprint for the future
Authors: Bruinsma Jeroen , Visser Leonie N. C. , Abaci Alara , Rosenberg Anna , Diaz Ana , Hanke Sten , Crutzen Rik , Mangialasche Francesca , Kivipelto Miia , Thunborg Charlotta
Published: May 2024

Introduction

Social activities are important for health and act as a driver of cognitive reserve during aging. In this perspective paper, we describe challenges and outline future (research) endeavors to establish better operationalization of social activities in multidomain interventions to prevent dementia.

Body

We first address the lack of conceptual clarity, which makes it difficult to measure engagement in social activities. Second, drawing from our experience with the Finnish Geriatric Intervention Study to Prevent Cognitive Impairment and Disability (FINGER), we discuss social activities in multidomain dementia prevention interventions. Using qualitative data from the Multimodal Preventive Trial for Alzheimer’s Disease (MIND-ADmini), we reflect on participant experiences with social activities. Third, we address the potential and challenges of digital solutions in promoting social activities in interventions for dementia prevention. Finally, we share insights from a workshop on digital technology, where we consulted with individuals with and without cognitive impairment who have been involved in three European projects (i.e., EU-FINGERS, Multi-MeMo, and LETHE).

Discussion

Based on these insights, we advocate for research that strengthens and accelerates the integration of social activities into multidomain interventions for dementia prevention. We propose several ways to achieve this: (a) by conducting mixed methods research to formulate a broadly accepted definition and instructions to measure social activities; (b) by focusing on promoting engagement in social activities beyond the intervention setting; and (c) by exploring the needs and preferences of older adults towards digitally-supported interventions and co-design of new technologies that enrich in-person social activities.
Transgressive incidents targeted on staff in forensic psychiatric healthcare: a latent class analysis
Authors: Frowijn Iris , Masthoff Erik , Vermunt Jeroen K. , Bogaerts Stefan
Published: May 2024

Transgressive incidents directed at staff by forensic patients occur frequently, leading to detrimental psychological and physical harm, underscoring urgency of preventive measures. These incidents, emerging within therapeutic relationships, involve complex interactions between patient and staff behavior. This study aims to identify clusters of transgressive incidents based on incident characteristics such as impact, severity, (presumed) cause, type of aggression, and consequences, using latent class analysis (LCA). Additionally, variations in incident clusters based on staff, patient, and context characteristics were investigated. A total of 1,184 transgressive incidents, reported by staff and targeted at staff by patients between 2018-2022, were extracted from a digital incident reporting system at Fivoor, a Dutch forensic psychiatric healthcare organisation. Latent Class Analysis revealed six incident classes: 1) verbal aggression with low impact; 2) verbal aggression with medium impact; 3) physical aggression with medium impact; 4) verbal menacing/aggression with medium impact; 5) physical aggression with high impact; and 6) verbal and physical menacing/aggression with high impact. Significant differences in age and gender of both staff and patients, staff function, and patient diagnoses were observed among these classes. Incidents with higher impact were more prevalent in high security clinics, while lower-impact incidents were more common in clinics for patients with intellectual disabilities. Despite limitations like missing information, tailored prevention approaches are needed due to varying types of transgressive incidents across patients, staff, and units.
Cross-sectional study on the epidemiological investigation ability of professional staff from Centers for Disease Control and Prevention in Guizhou Province
Authors: Huang He , Yao Guang-hai , Huang Jiao , Deng Bin , Wu Jun , Yu Chun
Published: May 2024

Introduction

This study aims to evaluate the qualifications and identify skill enhancement areas for epidemiological investigators in Centers for Disease Control and Prevention (CDCs) in Guizhou’s, informing future training and policy initiatives to strengthen public health responses.

Methods

A cross-sectional survey was conducted in August 2022, and an online, self-designed questionnaire on the Epidemiological Dynamic Data Collection platform was administered to evaluate the professional staff in CDCs. The responses were scored and presented using descriptive statistical methods, and the factors influencing the total score were analyzed by one-way ANOVA and linear regression.

Results and discussion

A total of 1321 questionnaires were collected, yielding an average score of 14.86±3.49 and a qualification rate of 29.9%. The scoring rate of ability of individual protection and coordination in epidemic control was high (87.25%). Meanwhile, improvements in further training were needed in areas such as data analysis ability (23.67%), knowledge of site disinfection (40.40%), and epidemiological investigation skills (42.50%). No significant difference was observed between the scores of city and county CDCs, (t = 1.071, p =0.284). The effects of gender and age could be disregarded, and the experience in epidemiological work and training (including investigation on COVID-19 cases and contacts), educational background, and professional title partially explained the survey outcome (R Square of the linear regression model was 0.351). The survey indicated the need for additional well-trained epidemiologic investigators in Guizhou. Specified training was effective in improving epidemiologic investigation, and enhancement in data analysis ability and knowledge of field disinfection are recommended in professional staff cultivation.
Pay-for-performance and low back pain with interaction of overwork: findings from the cross-sectional Korean working conditions survey
Authors: Hur Julia D. , Lee Jongin
Published: May 2024

Background

Pay-for-performance (PFP) is a type of incentive system where employees receive monetary rewards for meeting predefined standards. While previous research has investigated the relationship between PFP and health outcomes, the focus has primarily been on mental health. Few studies have explored the impact of PFP on specific physical symptoms like pain.

Methods

Data from the Korean Working Conditions Survey (KWCS) was analyzed, encompassing 20,815 subjects with information on PFP and low back pain (LBP). The associations between types of base pay (BP) and PFP with LBP were examined using multivariate logistic regression models, taking into account a directed acyclic graph (DAG). The interaction of overtime work was further explored using stratified logistic regression models and the relative excess risk for interaction.

Results

The odds ratio (OR) for individuals receiving both BP and PFP was statistically significant at 1.19 (95% CI 1.04–1.35) compared to those with BP only. However, when the DAG approach was applied and necessary correction variables were adjusted, the statistical significance indicating a relationship between PFP and LBP vanished. In scenarios without PFP and with overtime work, the OR related to LBP was significant at 1.54 (95% CI 1.35–1.75). With the presence of PFP, the OR increased to 2.02 (95% CI 1.66–2.45).

Conclusion

Pay-for-performance may influence not just psychological symptoms but also LBP in workers, particularly in conjunction with overtime work. The impact of management practices related to overtime work on health outcomes warrants further emphasis in research.
The effect of the initial distribution of labor-related property rights on the allocative efficiency of labor markets
Authors: Dietl Helmut M. , Lang Markus , Orlowski Johannes , Wegelin Philipp
Published: May 2024

Introduction

The Coase Theorem posits that frictionless markets efficiently allocate scarce resources as long as property rights are fully specified. Our empirical study investigates how the initial allocation of labor-related property rights influences the allocative efficiency in labor markets for skilled workers within a highly competitive environment—professional basketball. Specifically, we compare two regimes: one where employers can trade workers to other employers without the worker's consent, and another where workers are free agents, able to negotiate and move freely without their employer's consent.

Methods

We utilize the NBA as a “laboratory” to conduct our analysis, constructing a unique panel dataset that includes 3,132 player-season observations spanning 17 regular seasons from 2003/04 to 2019/20. To address our research question, we employ linear panel regression models to analyze the data.

Results and discussion

The findings reveal a decline in productivity among workers who transition to new employers as free agents, a phenomenon not observed among non-free agents. This observation suggests that allocative efficiency might be higher when workers are traded without their consent compared to when they exercise their autonomy as free agents. These findings highlight the significant impact that the initial distribution of labor-related property rights has on labor market efficiency, potentially challenging the assumptions of the Coase Theorem. However, the lack of a statistically significant difference in productivity changes between free agents and non-free agents moving to new employers prevents us from definitively rejecting the predictions of the Coase Theorem.
Distributed training of CosPlace for large-scale visual place recognition
Authors: Zaccone Riccardo , Berton Gabriele , Masone Carlo
Published: May 2024

Visual place recognition (VPR) is a popular computer vision task aimed at recognizing the geographic location of a visual query, usually within a tolerance of a few meters. Modern approaches address VPR from an image retrieval standpoint using a kNN on top of embeddings extracted by a deep neural network from both the query and images in a database. Although most of these approaches rely on contrastive learning, which limits their ability to be trained on large-scale datasets (due to mining), the recently reported CosPlace proposes an alternative training paradigm using a classification task as the proxy. This has been shown to be effective in expanding the potential of VPR models to learn from large-scale and fine-grained datasets. In this work, we experimentally analyze CosPlace from a continual learning perspective and show that its sequential training procedure leads to suboptimal results. As a solution, we propose a different formulation that not only solves the pitfalls of the original training strategy effectively but also enables faster and more efficient distributed training. Finally, we discuss the open challenges in further speeding up large-scale image retrieval for VPR.
Strobe training as a visual training method that improves performance in climbing
Authors: Vasile Antonia Ioana , Stănescu Monica Iulia
Published: May 2024

Introduction

Strobe training is a form of visual training where the athlete has to practice during intermittently dark conditions. Strobe training improves visual, perceptual, and cognitive skills, which will enhance athletic performance. Strobe training can influence multiple training components in climbing: psychological, tactical, physical, and technical training.

Materials and methods

The study was conducted on 17 elite climbers from Romania (10 male and 7 female), representing the entire National Youth Climbing Team. The research group was divided into a control group (n = 8) and an experimental group (n = 9). The used instruments were the Cognitrom battery (for cognitive skills, such as spatial skills and reactivity), the Witty SEM system (for motor-cognitive skills, such as cognitive agility, visual processing speed, and visual memory), and the International Rock Climbing Research Association (IRCRA) performance-related test battery for climbers (climbing-specific motor skills). The experimental group had 20 strobe training sessions, which took place during one calendar year, as an additional session to their climbing schedule done with their principal trainer. The strobe session was once a week, depending on the periodization of the macrocycle (preparatory, competitional, and transition periods). The control group and the experimental group had similar climbing training sessions during the 1-year macrocycle in terms of intensity and volume of their training.

Results

Strobe training improved on-sight performance (d = 0.38) and red-point performance (d = 0.36). Strobe training improved the majority of cognitive skills [all spatial skills (d = 1.27 for mental image transformation;d = 1.14 for spatial orientation;d = 1.59 for image generation) and simple reaction time (d = 0.99)]. Strobe training improved all motor-cognitive skills (d = 0.16 for visual memory;d = 1.96 for visual memory errors;d = 1.39 for visual processing speed;d = 1.94 for visual processing errors;d = 1.30 for cognitive agility). Strobe training improved many climbing-specific parameters (flexibility and upper body strength) (d = 0.44 andd = 0.47 for flexibility parameters;d = 0.50 to 0.73 for upper body strength parameters).

Discussion

Strobe training is an effective training method for enhancing performance that should be used on more experienced climbers. It acts more on spatial skills, rather than on reactivity skills, developing the visual-motor coordination system. Strobe training has greater effects on climbers aged below 16 years, as youth athletes rely more on visual input compared to adults. The improvement in climbing-specific variables was due to the additional climbing session done weekly. Strobe training acts more on the cognitive component of training than on the motor component of training in climbing.
Pulmonary responses following cardiac rehabilitation and the relationship with functional outcomes in children and young adults with heart disease
Authors: Kershner Cassidy E. , Hardie William D. , Chin Clifford , Opotowsky Alexander R. , Aronoff Elizabeth B. , Mays Wayne A. , Knecht Sandra K. , Powell Adam W.
Published: May 2024

Introduction

Patients with congenital heart disease (CHD) often have pulmonary abnormalities and exercise intolerance following cardiac surgery. Cardiac rehabilitation (CR) improves exercise capacity in patients with CHD, but minimal study has been performed to see if resting and dynamic pulmonary performance improves following CR in those with prior cardiac surgery.

Methods

This was a retrospective cohort study of all patients who completed ≥12 weeks of CR from 2018 through 2022. Demographic, cardiopulmonary exercise test (CPET), spirometry, 6-minute walk, functional strength measures, and outcomes data were collected. Data are presented as median[IQR]. A Student's t-test was used for comparisons between groups and serial measurements were measured with a paired t-test. A p < 0.05 was considered significant.

Results

There were a total of 37 patients [age 16.7 (14.2–20.1) years; 46% male] included. Patients with prior surgery (n = 26) were more likely to have abnormal spirometry data than those without heart disease (n = 11) (forced vital capacity [FVC] 76.7 [69.1–84.3]% vs. 96.4 [88.1–104.7]%, p = 0.002), but neither group experienced a significant change in spirometry. On CPET, peak oxygen consumption increased but there was no change in other pulmonary measures during exercise. Percent predicted FVC correlated with hand grip strength (r = 0.57, p = 0.0003) and percent predicted oxygen consumption (r = 0.43, p = 0.009). The number of prior sternotomies showed negative associations with both percent predicted FVC (r = −0.43, p = 0.04) and FEV1 (r = −0.47, p = 0.02).

Discussion

Youth and young adults with a prior history of cardiac surgery have resting and dynamic pulmonary abnormalities that do not improve following CR. Multiple sternotomies are associated with worse pulmonary function.
Osteoarthritis, adipokines and the translational research potential in small animal patients
Authors: Theyse Lars F. H. , Mazur Emilia M.
Published: May 2024

Osteoartritis (OA) is a debilitating disease affecting both humans and animals. In the early stages, OA is characterized by damage to the extracellular matrix (ECM) and apoptosis and depletion of chondrocytes. OA progression is characterized by hyaline cartilage loss, chondrophyte and osteophyte formation, thickening of the joint capsule and function loss in the later stages. As the regenerative potential of cartilage is very limited and osteoarthritic changes are irreversible, prevention of OA, modulation of existing osteoarthritic joint inflammation, reducing joint pain and supporting joint function are the only options. Progression of OA and pain may necessitate surgical intervention with joint replacement or arthrodesis as end-stage procedures. In human medicine, the role of adipokines in the development and progression of OA has received increasing interest. At present, the known adipokines include leptin, adiponectin, visfatin, resistin, progranulin, chemerin, lipocalin-2, vaspin, omentin-1 and nesfatin. Adipokines have been demonstrated to play a pivotal role in joint homeostasis by modulating anabolic and catabolic balance, autophagy, apoptosis and inflammatory responses. In small animals, in terms of dogs and cats, naturally occurring OA has been clearly demonstrated as a clinical problem. Similar to humans, the etiology of OA is multifactorial and has not been fully elucidated. Humans, dogs and cats share many joint related degenerative diseases leading to OA. In this review, joint homeostasis, OA, adipokines and the most common joint diseases in small animals leading to naturally occurring OA and their relation with adipokines are discussed. The purpose of this review is highlighting the translational potential of OA and adipokines research in small animal patients.
Biomarkers of in vivo platelet activation in thoroughbreds during their first long-term training
Authors: Miglio Arianna , Falcinelli Emanuela , Mezzasoma Anna Maria , Busechian Sara , Rueca Fabrizio , Gresele Paolo , Antognoni Maria Teresa
Published: May 2024

Physical exercise has an activating effect on platelet function that differs between trained and untrained subjects, depending on the type of exercise and training status. In humans, soluble P-selectin (sP-sel) and platelet-derived extracellular vesicles (PEVs) are considered reliable markers of in vivo platelet activation during exercise. In untrained humans, they increase after transient physical exercise, whereas long-term training induces a decrease in their resting levels due to an improved ability to adapt to hemodynamic changes. The aim of this study was to assess whether circulating levels of sP-sel and PEVs may be useful markers to explore in vivo platelet function in never-trained Thoroughbreds during their first 4 months of incremental training. A total of 29 clinically healthy, untrained Thoroughbreds (17 males and 12 females) were enrolled. All horses were trained with the same training schedule (90 days). Blood samples were collected on the day the training program began (T0), 30 days (T30), and 90 days (T90) after its incremental increase to quantify platelet count, sP-sel (horse enzyme-linked immunosorbent assay) and PEVs (flow cytometry). Statistical analysis was performed using RM one-way analysis of variance with the Geisser–Greenhouse correction. Soluble P-selectin tended to increase at T30 compared with T0, while T90 levels returned to baseline values. Significantly higher circulating levels of PEVs CD61+/AnnV+ were observed at T30 and T90 compared to baseline confirming platelet hyperactivity. The detection and quantification of sP-sel and PEVs in equine racehorses during the training period appears to be a promising tool to study exercise-induced primary hemostatic changes and may provide an important marker for exercise selection.
Greenspaces and Health: Scoping Review of studies in Europe
Authors: Banwell Nicola , Michel Sarah , Senn Nicolas
Published: May 2024

Objectives

Access to greenspaces and contact with nature can promote physical activity and have positive effects on physical and mental health. This scoping literature review aims to examine current evidence linking greenspaces and (a) behaviour change, (b) health outcomes and (c) co-benefits.

Methods

This review was conducted in accordance with the PRISMA scoping review guidelines. Searches were conducted through PubMed and EMBASE databases for studies published between 2000 and March 2023 with a focus on Europe.

Results

122 scientific articles and grey literature reports were identified. Access to greenspaces is positively associated with physical and mental health, and reduced risk of all-cause mortality and some non-communicable diseases. Greenspace quality is associated with increased physical activity and reduced risk of obesity. Nature-based therapies or green prescription are effective in improving mental health outcomes and overall health. Importantly, numerous co-benefits of greenspaces are identified.

Conclusion

Increasing access to greenspaces for populations with particular attention to greenspace quality is important for co-benefits. Responsible governance and use of greenspaces are crucial to minimize public health risks and human disturbance of nature.
An Incubation System for the Simulation of Ammonia Emissions from Soil Surface-Applied Slurry: Effect of pH and Acid Type
Authors: Ellersiek Nils Carsten Thomas , Olfs Hans-Werner
Published: May 2024

Acidification of slurry is a promising approach for reducing ammonia emissions during the application procedure. Since only a few studies have been conducted focusing on ammonia emissions during the application of liquid organic fertilizers on the soil surface, a suitable incubation system was developed to evaluate the effects of acidification under controlled conditions. This incubation system was used to measure the ammonia emissions of various liquid organic fertilizers. The substrates were acidified with sulfuric and citric acid to different pH values to determine both the influence of the pH value of the substrates and of the type of acid on the ammonia emissions. The emissions decreased with declining pH value, and the reduction in emissions compared to the initial pH of the substrate was over 86% for pH 6.5 and over 98% for pH 6.0 and below. At the same pH value, the ammonia emissions did not differ between substrates acidified with citric acid and sulfuric acid, although more than twice as much 50% citric acid was required compared to 96% sulfuric acid to achieve the same pH value. Overall, our results demonstrate that the incubation system used is suitable for measuring ammonia emissions from surface-applied liquid organic fertilizers. The system allows for the differentiation of emission levels at various pH levels and is therefore suitable for testing the effectiveness of additives for reducing ammonia emissions from liquid organic fertilizers.
The Best Protocol to Treat Equine Skin Wounds by Second Intention Healing: A Scoping Review of the Literature
Authors: Ribeiro Gesiane , Carvalho Lúcia , Borges João , Prazeres José
Published: May 2024

Equine skin wound treatment continues to be a challenge for veterinarians. Despite being a frequent practice, it remains difficult to choose an evidence-based treatment protocol. This study aimed to comprehensively explore the literature and provide a scoping review of therapeutic strategies for equine skin wounds and identify knowledge gaps and opportunities for future research. This review was conducted using specific criteria to select literature that described methods to manage second intention wound healing. After removing duplicates and screening papers for suitability, 81 manuscripts were included for data extraction. Of these, 59 articles were experimental studies, 10 were case reports, 9 were case series, and 3 were clinical studies. The most frequent wound location was the distal limbs. Macroscopic assessment was the main tool used to evaluate treatment effectiveness. All of the case reports, case series, and clinical studies reported positive outcomes with regard to the treatment used, while only 36% of the experimental studies found significant healing improvement in treated wounds compared to control groups. It was found that there are many treatments that have exhibited controversial results, and there exists a lack of evidence for the adoption of specific treatment protocols.
Seasonal Effects on Health Status and Parasitological Traits of an Invasive Minnow in Iberian Waters
Authors: Almeida David , Alcaraz-Hernández Juan Diego , Cruz Alejandra , Lantero Esther , Fletcher David H. , García-Berthou Emili
Published: May 2024

Biological invasions are of special conservation concern in the Iberian Peninsula and other regions with high levels of endemism. Environmental variability, such as the seasonal fluctuations of Mediterranean streams, is a key factor that affects the spread of aquatic species in novel habitats. Fish parasites have a great potential to reflect such changes in the habitat features of freshwater ecosystems. The aim of this study consisted of seasonally analysing the health status and parasitological traits of non-native fish in Iberian waters. In particular, a strongly invasive population of Languedoc minnow Phoxinus septimaniae (leuciscid species native to south-east France) was assessed in Tordera Stream (north-eastern Iberian Peninsula, Mediterranean conditions). Fish were sampled in April, July, and October 2023 by electrofishing. Health status (external/internal organs) was significantly better in autumn (HAI = 28.8) than spring (HAI = 35.6). Life-cycle complexity was higher in spring (LCI = 1.98), whereas parasite abundance and Shannon diversity were significantly lower in autumn (TA = 19.6 and H’ = 2.15, respectively). In October (more ‘benign’ environmental conditions in Iberian streams), minnows could display elevated foraging activity, with fish increasing their health condition and level of parasite resistance/tolerance. Overall results showed a particular seasonal profile of health and parasite infra-communities that allow this minnow species to thrive under highly fluctuating habitat conditions. This information could help environmental managers to control non-native fish in Mediterranean streams.
Rearing Management and Its Impact on Caseous Lymphadenitis in Sheep
Authors: El Khalfaoui Nora , El Amiri Bouchra , Cabaraux Jean-François , Chentouf Mouad , Raes Marianne , Marcotty Tanguy , Kirschvink Nathalie
Published: May 2024

Two surveys were conducted to assess the prevalence and risk factors of ovine caseous lymphadenitis (CL) and explore the association between its superficial and visceral forms in Sardi sheep in Settat province, Morocco. A total of 1521 sheep, including 318 lambs < 6 months, 572 young sheep aged 6–24 months, and 631 adult sheep > 24 months, were examined from 57 flocks. Superficial CL prevalence levels were as follows: 69/318 in lambs, 292/572 in young sheep, and 114/631 in adult sheep. Additionally, 2124 sheep, including 1813 young and 311 adult sheep, were inspected in slaughterhouses ante- and post-mortem. Among all infected animals, the prevalence of visceral abscesses was notably higher in adult sheep (83%, OR = 9.8, 95% CI = 5.5–17.2) compared to young sheep (35%). Data on flock size, sex, age, body condition score, rearing methods, and hygienic measures were collected. Suspected CL cases were confirmed using quadruplex-PCR. Poor barn ventilation, lack of abscess control, and younger age were identified as key risk factors for CL. Additionally, a high percentage (89%) of animals with thoracic abscesses did not display superficial lesions. While age and abscess control have been reported in previous studies, barn ventilation appears to be a new, but important, risk factor. In conclusion, the identified risk factors in Settat province are linked to breeding management practices. Implementing regular abscess control measures and improving barn ventilation are essential for CL prevention.