bluuebunny/crossref_metadata_2025 huggingface dataset:
Techno-Economic Investigation of Solar Powered Electric Auto-Rickshaw for a Sustainable Transport System
Authors: Reddy K. , Aravindhan S. , Mallick Tapas
Published: May 2017

Technologies influencing alternative means of transportation have been expanding in recent years due to increasing urbanization and motorization. In this paper, a solar powered electric auto-rickshaw (SPEA) is designed and developed for Indian conditions. The vehicle developed is comprehensively analyzed techno-economically for its viability in the Indian market. The performance analysis of SPEA results in an optimal charging rate of 2 kWh per day with an average solar irradiance of 325 W/m2 on a typical sunny day. The discharging characteristics are studied based on different loading conditions. The vehicle achieved a maximum speed of 21.69 km/h with battery discharge rate of 296 W at 90 kg load and also reached a maximum discharge rate of 540 W at 390 kg loading with a maximum speed of 12.11 km/h. Environmental analysis of SPEA indicated that the yearly CO2 emissions of 1777 kg, 1987 kg and 1938 kg from using Compressed Natural Gas, Liquefied Petroleum Gas and gasoline engines respectively can be mitigated using SPEA. The financial analysis of SPEA concluded that the investor’s payback duration is 24.44% less compared to a gasoline-run vehicle. Socio-Economic analysis of SPEA discussed its significant advantages and showed 15.74% and 0.85% increase in yearly income over gasoline driven and battery driven vehicles.
Optimizing the Renewable Energy Potential: Myth or Future Trend in Romania
Authors: Oncioiu Ionica , Petrescu Anca , Grecu Eugenia , Petrescu Marius
Published: May 2017

This paper investigates the potential of Romanian renewable energy across energy production and consumption definitions. Moreover, it presents a model that estimates the regional evolution of renewable energy diffusion, focusing primarily on hydro energy. Statistics showed that Romanian consumers have a key role in developing the renewable energy market, while it is also very important to ensure the ability of renewable energy suppliers to accommodate this demand. Sample data and econometric analysis were applied to delineate factors influencing Romanian consumers’ awareness about renewable energy. The findings highlighted a strong connection between those who bear the cost of renewable energy development and the actual beneficiaries of a clean environment; in that sense, they identify the major drivers and barriers for renewable energy diffusion in Romania. The results of this research could be used for further research in the area of implementing renewable energy projects in the region.
Characteristics of Fine Roots of Pinus massoniana in the Three Gorges Reservoir Area, China
Authors: Shen Yafei , Wang Na , Cheng Ruimei , Xiao Wenfa , Yang Shao , Guo Yan , Lei Lei , Zeng Lixiong , Wang Xiaorong
Published: May 2017

Several studies have focused on fine roots characteristics because they provide a major pathway for nutrient cycling and energy flow in forest ecosystems. However, few studies have evaluated changes in fine root characteristics according to their diameter. Pinus massoniana forests are the main vegetative component in the Three Gorges Reservoir area and play an important role in providing forest resources and ecological services. Pinus massoniana fine roots were sorted into 0–0.5, 0.5–1, and 1–2 mm diameter classes, and their fine root standing biomass (FRB), necromass, annual production and decomposition rates were determined and correlated with soil characteristics. These fine roots in three diameter classes significantly differed in their initial carbon (C), C/N ratio, FRB, necromass, annual C and N production and decomposition rate. The production and decomposition of these different diameter classes varied significantly with soil variables including soil temperature, moisture, calcium and ammonium concentration but the strength of these interactions varied dependent on diameter class. The very fine roots had a faster decomposition ratio than larger fine roots due to the lower N content, higher C/N ratio and higher sensitivity to soil environmental factors. These results clearly indicate heterogeneity among fine roots of different diameters, and these variations should be taken into account when studying fine root characteristics and their role in the C cycle.
Terrestrial Laser Scanning for Forest Inventories—Tree Diameter Distribution and Scanner Location Impact on Occlusion
Authors: Abegg Meinrad , Kükenbrink Daniel , Zell Jürgen , Schaepman Michael , Morsdorf Felix
Published: May 2017

The rapid development of portable terrestrial laser scanning (TLS) devices in recent years has led to increased attention to their applicability for forest inventories, especially where direct measurements are very expensive or nearly impossible. However, in terms of precision and reproducibility, there are still some pending questions. In this study, we investigate the influence of stand parameters on the TLS-related visibility in forest plots. We derived 2740 stand parameters from Swiss national forest inventory sample plots. Based on these parameters, we defined virtual scenes of the forest plots with the software “Blender”. Using Blender’s ray-tracing features, we assessed the 3D coverage in a cubic space and 2D visibility properties for each of the virtual plots with different scanner placement schemes. We provide a formula to calculate the maximum number of possible hits for any object size at any distance from a scanner with any resolution. Additionally, we show that the Weibull scale parameter describing a stand, in addition to the number of trees and the mean diameter of the dominant 100 trees per hectare, has a significant and relevant influence on the visibility of the sample plot. Furthermore, we show the effectiveness and the efficiency of 40 scanner location patterns. These experiments demonstrate that intuitively distributing scanner locations evenly within the sample plot, with similar distances between locations and from the edge of the sample plot, provides the best overall visibility of the stand.
Characterizing Habitat Elements and Their Distribution over Several Spatial Scales: The Case of the Fisher
Authors: Niblett Matthew , Church Richard , Sweeney Stuart , Barber Klaus
Published: May 2017

In past studies of the fisher (Pekania pennanti) most researchers have concluded that fisher habitat must consist of mostly mature to late-seral forest with few, if any, openings. Without doubt, certain elements found in mature to late-seral forests are required by females to successfully rear their young, but some recent work casts doubt on the extent that a continuous canopy of tree coverage and a preponderance of older stands are necessary as long as certain components exist. This paper explores this issue with an attempt to better characterize essential elements of habitat for the female fisher. This characterization is based upon fine-scale inventory plot data that is analyzed across several spatial scales that represent a small neighborhood about den sites, the forest of the 75% kernel density estimate for female home ranges, and the forested region as a whole. We present results of a test of significance in comparing habitat elements across these three scales. Our findings suggest that certain habitat elements typically found in mature to late seral forests must be present at a certain fraction of the landscape for the fisher. The approach described here may be of considerable value in developing guidelines for conservation agreements.
Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets
Authors: Battista Edmondo , Causa Filippo , Netti Paolo
Published: May 2017

Hydrogels, and in particular microgels, are playing an increasingly important role in a diverse range of applications due to their hydrophilic, biocompatible, and highly flexible chemical characteristics. On this basis, solution-like environment, non-fouling nature, easy probe accessibility and target diffusion, effective inclusion of reporting moieties can be achieved, making them ideal substrates for bio-sensing applications. In fact, hydrogels are already successfully used in immunoassays as well as sensitive nucleic acid assays, also enabling hydrogel-based suspension arrays. In this review, we discuss key parameters of hydrogels in the form of micron-sized particles to be used in sensing applications, paying attention to the protein and oligonucleotides (i.e., miRNAs) targets as most representative kind of biomarkers.
DNA Methylation Profiling of Human Prefrontal Cortex Neurons in Heroin Users Shows Significant Difference between Genomic Contexts of Hyper- and Hypomethylation and a Younger Epigenetic Age
Authors: Kozlenkov Alexey , Jaffe Andrew , Timashpolsky Alisa , Apontes Pasha , Rudchenko Sergei , Barbu Mihaela , Byne William , Hurd Yasmin , Horvath Steve , Dracheva Stella
Published: May 2017

We employed Illumina 450 K Infinium microarrays to profile DNA methylation (DNAm) in neuronal nuclei separated by fluorescence-activated sorting from the postmortem orbitofrontal cortex (OFC) of heroin users who died from heroin overdose (N = 37), suicide completers (N = 22) with no evidence of heroin use and from control subjects who did not abuse illicit drugs and died of non-suicide causes (N = 28). We identified 1298 differentially methylated CpG sites (DMSs) between heroin users and controls, and 454 DMSs between suicide completers and controls (p < 0.001). DMSs and corresponding genes (DMGs) in heroin users showed significant differences in the preferential context of hyper and hypo DM. HyperDMSs were enriched in gene bodies and exons but depleted in promoters, whereas hypoDMSs were enriched in promoters and enhancers. In addition, hyperDMGs showed preference for genes expressed specifically by glutamatergic as opposed to GABAergic neurons and enrichment for axonogenesis- and synaptic-related gene ontology categories, whereas hypoDMGs were enriched for transcription factor activity- and gene expression regulation-related terms. Finally, we found that the DNAm-based “epigenetic age” of neurons from heroin users was younger than that in controls. Suicide-related results were more difficult to interpret. Collectively, these findings suggest that the observed DNAm differences could represent functionally significant marks of heroin-associated plasticity in the OFC.
Sleep Duration, Sleep Quality, Body Mass Index, and Waist Circumference among Young Adults from 24 Low- and Middle-Income and Two High-Income Countries
Authors: Peltzer Karl , Pengpid Supa
Published: May 2017

Obesity and its comorbidities have emerged as a leading public health concern. The aim of this study was to explore the relationship between body mass index (BMI), waist circumference (WC) and sleep patterns, including duration and disturbances. A cross-sectional questionnaire survey and anthropometric measurements were conducted with undergraduate university students that were randomly recruited in 26 universities in 24 low- and middle-income and two high-income countries. The sample included 18,211 (42.1% male and 57.9% female, mean age 21.0 in male and 20.7 years in female students) undergraduate university students. The overall BMI was a mean of 22.5 kg/m2 for men and 22.0 kg/m2 for women, and the mean WC was 78.4 cm for men and 73.8 cm for women. More than 39% of the students reported short sleep duration (≤6 h/day) and over 30% reported moderate to extreme sleep problems. In a linear multivariable regression, adjusted for sociodemographic and lifestyle factors, short sleep duration was positively associated with BMI in both men and women, and was positively associated with WC among women but not among men. Sleep quality or problems among men were not associated with BMI, while among women mild sleep problems were inversely associated with BMI, and poor sleep quality or problems were positively associated with WC both among men and women. The study confirmed an association between short sleep duration and increased BMI and, among women, increased WC, and an association between poor sleep quality and increased WC but not BMI. Further, differences in the association between sleep characteristics and BMI and WC were found by region and country income.
Is Housing a Health Insult?
Authors: Baker Emma , Beer Andrew , Lester Laurence , Pevalin David , Whitehead Christine , Bentley Rebecca
Published: May 2017

In seeking to understand the relationship between housing and health, research attention is often focussed on separate components of people’s whole housing ‘bundles’. We propose in this paper that such conceptual and methodological abstraction of elements of the housing and health relationship limits our ability to understand the scale of the accumulated effect of housing on health and thereby contributes to the under-recognition of adequate housing as a social policy tool and powerful health intervention. In this paper, we propose and describe an index to capture the means by which housing bundles influence health. We conceptualise the index as reflecting accumulated housing ‘insults to health’—an Index of Housing Insults (IHI). We apply the index to a sample of 1000 low-income households in Australia. The analysis shows a graded association between housing insults and health on all outcome measures. Further, after controlling for possible confounders, the IHI is shown to provide additional predictive power to the explanation of levels of mental health, general health and clinical depression beyond more traditional proxy measures. Overall, this paper reinforces the need to look not just at separate housing components but to embrace a broader understanding of the relationship between housing and health.
FADS Gene Polymorphisms, Fatty Acid Desaturase Activities, and HDL-C in Type 2 Diabetes
Authors: Huang Meng-Chuan , Chang Wen-Tsan , Chang Hsin-Yu , Chung Hsin-Fang , Chen Fang-Pei , Huang Ya-Fang , Hsu Chih-Cheng , Hwang Shang-Jyh
Published: May 2017

Polyunsaturated fatty acids (PUFA) correlate with risk of dyslipidemia and cardiovascular diseases. Fatty acid desaturase (FADS) single nucleotide polymorphisms (SNPs) modulate circulating PUFA concentrations. This study examined influence of FADS1 and FADS2 genetic variants on desaturase activities and blood lipid concentrations in type 2 diabetes patients, and further assessed their interrelationships. Selected SNPs (FADS1: rs174547, rs174548, rs174550; FADS2: rs174575, rs174576, rs174583, rs498793 and rs2727270) were genotyped in 820 type 2 diabetes patients and compared with those reported in the HapMap. Patient subgroups (n = 176) without taking lipid-lowering medicine were studied to assess influence of tag SNPs including rs174547, rs174575, rs498793 and rs2727270 on delta-5 desaturase (D5D: 20:4 (n-6)/20:3 (n-6)) and delta-6 desaturase (D6D:18:3 (n-6)/18:2 (n-6)) activities, and blood lipids. FADS1 rs174547 TT/TC/CC and FADS2 rs2727270 CC/CT/TT were significantly (p for trend < 0.05) associated with reduced HDL-C, D5D and D6D activities. Upon adjustment for confounders, D5D (p = 0.006) correlated significantly and D6D marginally (p = 0.07) correlated with increased HDL-C levels, whereas rs174547 and rs2727270 polymorphisms were not associated. D6D andD5D activities may play a role in modulating HDL-C levels in type 2 diabetes. Future studies with larger sample sizes are needed to investigate how FADS genetic variations interact with desaturase activities or PUFAs in the metabolism of lipoproteins in diabetic patients.
Functional Coping Dynamics and Experiential Avoidance in a Community Sample with No Self-Injury vs. Non-Suicidal Self-Injury Only vs. Those with Both Non-Suicidal Self-Injury and Suicidal Behaviour
Authors: Nielsen Emma , Sayal Kapil , Townsend Ellen
Published: May 2017

Although emotional avoidance may be a critical factor in the pathway from psychological distress to self-injury and/or suicidality, little is known about the relative importance of differing functional coping dynamics and experiential avoidance between people with self-injury histories of differing intent (e.g., Non-Suicidal Self-Injury only vs. Non-Suicidal Self-Injury plus Suicidal Behaviour; NSSI vs. NSSI + SB). A community-based survey (N = 313; female, 81%; ages 16–49 years, M = 19.78, SD = 3.48) explored self-reported experiential avoidance and functional coping dynamics in individuals with (i) no self-injury history (controls); (ii) a history of NSSI only; and (iii) a history of NSSI + SB. Jonckheere-Terpstra trend tests indicated that avoidance coping was higher in the NSSI and NSSI + SB groups than in controls. Emotion regulation was higher in controls than those with a history of self-injury (NSSI and NSSI + SB). Approach and reappraisal coping demonstrated significant ordered effects such that control participants were higher in these coping dynamics than those with a history of NSSI only, who, in turn, were higher than those with a history of NSSI + SB (Control > NSSI > NSSI + SB). Endorsement of the reappraisal/denial facet of experiential avoidance was most pronounced in those with a history of NSSI + SB (Control < NSSI < NSSI + SB). No significant ordered effects were observed for other dimensions of experiential avoidance. Understanding how the endorsement of functional coping dynamics and which components of experiential avoidance vary between groups with differing self-injury intent histories has important implications for treatment planning.
Prostaglandin E2-Induced COX-2 Expressions via EP2 and EP4 Signaling Pathways in Human LoVo Colon Cancer Cells
Authors: Hsu Hsi-Hsien , Lin Yueh-Min , Shen Chia-Yao , Shibu Marthandam , Li Shin-Yi , Chang Sheng-Huang , Lin Chien-Chung , Chen Ray-Jade , Viswanadha Vijaya , Shih Hui-Nung , Huang Chih-Yang
Published: May 2017

Metastasis is the most dangerous risk faced by patients with hereditary non-polyposis colon cancer (HNPCC). The expression of matrix metalloproteinases (MMPs) has been observed in several types of human cancers and regulates the efficacy of many therapies. Here, we show that treatment with various concentrations of prostaglandin E2 (PGE2; 0, 1, 5 or 10 μM) promotes the migration ability of the human LoVo colon cancer cell line. As demonstrated by mRNA and protein expression analyses, EP2 and EP4 are the major PGE2 receptors expressed on the LoVo cell membrane. The Phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt cell survival pathway was upregulated by EP2 and EP4 activation. Following the activation of the PI3K/Akt pathway, β-catenin translocated into the nucleus and triggered COX2 transcription via LEF-1 and TCF-4 and its subsequent translation. COX2 expression correlated with the elevation in the migration ability of LoVo cells. The experimental evidence shows a possible mechanism by which PGE2 induces cancer cell migration and further suggests PGE2 to be a potential therapeutic target in colon cancer metastasis. On inhibition of PGE2, in order to determine the downstream pathway, the levels of PI3K/Akt pathway were suppressed and the β-catenin expression was also modulated. Inhibition of EP2 and EP4 shows that PGE2 induces protein expression of COX-2 through EP2 and EP4 receptors in LoVo colon cancer cells.
A Relative Deficiency of Lysosomal Acid Lypase Activity Characterizes Non-Alcoholic Fatty Liver Disease
Authors: Tovoli Francesco , Napoli Lucia , Negrini Giulia , D’Addato Sergio , Tozzi Giulia , D’Amico Jessica , Piscaglia Fabio , Bolondi Luigi
Published: May 2017

Lysosomal acid lipase (LAL) is a key enzyme in lipid metabolism. Initial reports have suggested a role for a relative acquired LAL deficiency in non-alcoholic fatty liver disease (NAFLD)—however, it is still unclear whether this mechanism is specific for NAFLD. We aimed to determine LAL activity in a cohort of NAFLD subjects and in a control group of hepatitis C virus (HCV)-infected patients, investigating the role of liver cirrhosis. A total of 81 patients with a diagnosis of NAFLD, and 78 matched controls with HCV-related liver disease were enrolled. For each patient, LAL activity was determined on peripheral dried blood spots (DBS) and correlated with clinical and laboratory data. A subgroup analysis among cirrhotic patients was also performed. LAL activity is significantly reduced in NAFLD, compared to that in HCV patients. This finding is particularly evident in the pre-cirrhotic stage of disease. LAL activity is also correlated with platelet and white blood cell count, suggesting an analytic interference of portal-hypertension-induced pancytopenia on DBS-determined LAL activity. NAFLD is characterized by a specific deficit in LAL activity, suggesting a pathogenetic role of LAL. We propose that future studies on this topic should rely on tissue specific analyses, as peripheral blood tests are also influenced by confounding factors.
Perturbations in the Replication Program Contribute to Genomic Instability in Cancer
Authors: Blumenfeld Britny , Ben-Zimra Micha , Simon Itamar
Published: May 2017

Cancer and genomic instability are highly impacted by the deoxyribonucleic acid (DNA) replication program. Inaccuracies in DNA replication lead to the increased acquisition of mutations and structural variations. These inaccuracies mainly stem from loss of DNA fidelity due to replication stress or due to aberrations in the temporal organization of the replication process. Here we review the mechanisms and impact of these major sources of error to the replication program.
Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties
Authors: Mayr Judith , Grijalvo Santiago , Bachl Jürgen , Pons Ramon , Eritja Ramon , Díaz Díaz David
Published: May 2017

Three different ionene polymers with varying quaternary ammonium moieties were used as a proof of concept for the formulation of antisense oligonucleotides, which are capable of inhibiting Renilla luciferase messenger ribonucleic acid (mRNA). Cationic vesicles, consisting of cationic polymer, antisense oligonucleotide (Luc) and non-ionic surfactant polysorbate 80, were investigated regarding their ζ potential, cytotoxicity and transfection efficiency. Deoxyribonucleic acid- (DNA) forming complexes in the presence of cationic vesicles were also investigated in terms of small-angle X-ray scattering (SAXS). The studied cationic vesicles showed very little, if any, toxicity against HeLa cells. Transfection abilities proved to vary strongly depending on the present quaternary ammonium moiety.
Changes of Microrna Levels in Plasma of Patients with Rectal Cancer during Chemoradiotherapy
Authors: Jo Peter , Azizian Azadeh , Salendo Junius , Kramer Frank , Bernhardt Markus , Wolff Hendrik , Gruber Jens , Grade Marian , Beißbarth Tim , Ghadimi B. , Gaedcke Jochen
Published: May 2017

Since the response to chemoradiotherapy in patients with locally advanced rectal cancer is heterogeneous, valid biomarkers are needed to monitor tumor response. Circulating microRNAs are promising candidates, however analyses of circulating microRNAs in rectal cancer are still rare. 111 patients with rectal cancer and 46 age-matched normal controls were enrolled. The expression levels of 30 microRNAs were analyzed in 17 pre-treatment patients’ plasma samples. Differentially regulated microRNAs were validated in 94 independent patients. For 52 of the 94 patients a paired comparison between pre-treatment and post-treatment samples was performed. miR-17, miR-18b, miR-20a, miR-31, and miR-193a_3p, were significantly downregulated in pre-treatment plasma samples of patients with rectal cancer (p < 0.05). miR-29c, miR-30c, and miR-195 showed a trend of differential regulation. After validation, miR-31 and miR-30c were significantly deregulated by a decrease of expression. In 52 patients expression analyses of the 8 microRNAs in matched pre-treatment and post-treatment samples showed a significant decrease for all microRNAs (p < 0.05) after treatment. Expression levels of miR-31 and miR-30c could serve as valid biomarkers if validated in a prospective study. Plasma microRNA expression levels do not necessarily represent miRNA expression levels in tumor tissue. Also, expression levels of microRNAs change during multimodal therapy.
Anti-Inflammatory Properties of Brazilian Green Propolis Encapsulated in a γ-Cyclodextrin Complex in Mice Fed a Western-Type Diet
Authors: Rimbach Gerald , Fischer Alexandra , Schloesser Anke , Jerz Gerold , Ikuta Naoko , Ishida Yoshiyuki , Matsuzawa Ryota , Matsugo Seiichi , Huebbe Patricia , Terao Keiji
Published: May 2017

Ageing is often accompanied by chronic inflammation. A fat- and sugar-rich Western-type diet (WTD) may accelerate the ageing phenotype. Cell culture studies have indicated that artepillin C-containing Brazilian green propolis exhibits anti-inflammatory properties. However, little is known regarding its anti-inflammatory potential in mouse liver in vivo. In this study, female C57BL/6NRj wild-type mice were fed a WTD, a WTD supplemented with Brazilian green propolis supercritical extract (GPSE) encapsulated in γ-cyclodextrin (γCD) or a WTD plus γCD for 10 weeks. GPSE-γCD did not affect the food intake, body weight or body composition of the mice. However, mRNA levels of the tumour necrosis factor α were significantly downregulated (p < 0.05) in these mice compared to those in the WTD-fed controls. Furthermore, the gene expression levels of other pro-inflammatory markers, including serum amyloid P, were significantly (p < 0.001) decreased following GPSE-γCD treatment. GPSE-γCD significantly induced hepatic ferritin gene expression (p < 0.01), which may contribute to its anti-inflammatory properties. Conversely, GPSE-γCD did not affect the biomarkers of endogenous antioxidant defence, including catalase, glutathione peroxidase-4, paraoxonase-1, glutamate cysteine ligase and nuclear factor erythroid 2-related factor-2 (Nrf2). Overall, the present data suggest that dietary GPSE-γCD exhibits anti-inflammatory, but not antioxidant activity in mouse liver in vivo. Thus, GPSE-γCD has the potential to serve as a natural hepatoprotective bioactive compound for dietary-mediated strategies against chronic inflammation.
Mutual Regulation of NOD2 and RIG-I in Zebrafish Provides Insights into the Coordination between Innate Antibacterial and Antiviral Signaling Pathways
Authors: Nie Li , Xu Xiao-Xiao , Xiang Li-Xin , Shao Jian-Zhong , Chen Jiong
Published: May 2017

Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) and retinoic acid-inducible gene I (RIG-I) are two important cytosolic pattern recognition receptors (PRRs) in the recognition of pathogen-associated molecular patterns (PAMPs), initiating innate antibacterial and antiviral signaling pathways. However, the relationship between these PRRs, especially in teleost fish models, is rarely reported. In this article, we describe the mutual regulation of zebrafish NOD2 (DrNOD2) and RIG-I (DrRIG-I) in innate immune responses. Luciferase assays were conducted to determine the activation of NF-κB and interferon signaling. Morpholino-mediated knockdown and mRNA-mediated rescue were performed to further confirm the regulatory roles between DrNOD2 and DrRIG-I. Results showed that DrNOD2 and DrRIG-I shared conserved structural hallmarks with their mammalian counterparts, and activated DrRIG-I signaling can induce DrNOD2 production. Surprisingly, DrNOD2-initiated signaling can also induce DrRIG-I expression, indicating that a mutual regulatory mechanism may exist between them. Studies conducted using HEK293T cells and zebrafish embryos showed that DrRIG-I could negatively regulate DrNOD2-activated NF-κB signaling, and DrNOD2 could inhibit DrRIG-I-induced IFN signaling. Moreover, knocking down DrRIG-I expression by morpholino could enhance DrNOD2-initiated NF-κB activation, and vice versa, which could be rescued by their corresponding mRNAs. Results revealed a mutual feedback regulatory mechanism underlying NOD2 and RIG-I signaling pathways in teleosts. This mechanism reflects the coordination between cytosolic antibacterial and antiviral PRRs in the complex network of innate immunity.
Sensitivity of HOXB13 as a Diagnostic Immunohistochemical Marker of Prostatic Origin in Prostate Cancer Metastases: Comparison to PSA, Prostein, Androgen Receptor, ERG, NKX3.1, PSAP, and PSMA
Authors: Kristiansen Ilka , Stephan Carsten , Jung Klaus , Dietel Manfred , Rieger Anja , Tolkach Yuri , Kristiansen Glen
Published: May 2017

Aims: Determining the origin of metastases is an important task of pathologists to allow for the initiation of a tumor-specific therapy. Recently, homeobox protein Hox-B13 (HOXB13) has been suggested as a new marker for the detection of prostatic origin. The aim of this study was to evaluate the diagnostic sensitivity of HOXB13 in comparison to commonly used immunohistochemical markers for prostate cancer. Materials and methods: Histologically confirmed prostate cancer lymph node metastases from 64 cases were used to test the diagnostic value of immunohistochemical markers: prostate specific antigen (PSA), Prostatic acid phosphatase (PSAP), prostate specific membrane antigen (PSMA), homeobox gene NKX3.1, prostein, androgen receptor (AR), HOXB13, and ETS-related gene (ERG). All markers were evaluated semi-quantitatively using Remmele's immune reactive score. Results: The detection rate of prostate origin of metastasis for single markers was 100% for NKX3.1, 98.1% for AR, 84.3% for PSMA, 80.8% for PSA, 66% for PSAP, 60.4% for HOXB13, 59.6% for prostein, and 50.0% for ERG. Conclusions: Our data suggest that HOXB13 on its own lacks sensitivity for the detection of prostatic origin. Therefore, this marker should be only used in conjunction with other markers, preferably the highly specific PSA. The combination of PSA with NKX3.1 shows a higher sensitivity and thus appears preferable in this setting.
The Methods of Choice for Extracellular Vesicles (EVs) Characterization
Authors: Szatanek Rafal , Baj-Krzyworzeka Monika , Zimoch Jakub , Lekka Malgorzata , Siedlar Maciej , Baran Jarek
Published: May 2017

In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization.
Impaired Insulin Signaling is Associated with Hepatic Mitochondrial Dysfunction in IR+/−-IRS-1+/− Double Heterozygous (IR-IRS1dh) Mice
Authors: Franko Andras , Kunze Alexander , Böse Marlen , Von Kleist-Retzow Jürgen-Christoph , Paulsson Mats , Hartmann Ursula , Wiesner Rudolf
Published: May 2017

Mitochondria play a pivotal role in energy metabolism, but whether insulin signaling per se could regulate mitochondrial function has not been identified yet. To investigate whether mitochondrial function is regulated by insulin signaling, we analyzed muscle and liver of insulin receptor (IR)+/−-insulin receptor substrate-1 (IRS-1)+/− double heterozygous (IR-IRS1dh) mice, a well described model for insulin resistance. IR-IRS1dh mice were studied at the age of 6 and 12 months and glucose metabolism was determined by glucose and insulin tolerance tests. Mitochondrial enzyme activities, oxygen consumption, and membrane potential were assessed using spectrophotometric, respirometric, and proton motive force analysis, respectively. IR-IRS1dh mice showed elevated serum insulin levels. Hepatic mitochondrial oxygen consumption was reduced in IR-IRS1dh animals at 12 months of age. Furthermore, 6-month-old IR-IRS1dh mice demonstrated enhanced mitochondrial respiration in skeletal muscle, but a tendency of impaired glucose tolerance. On the other hand, 12-month-old IR-IRS1dh mice showed improved glucose tolerance, but normal muscle mitochondrial function. Our data revealed that deficiency in IR/IRS-1 resulted in normal or even elevated skeletal muscle, but impaired hepatic mitochondrial function, suggesting a direct cross-talk between insulin signaling and mitochondria in the liver.
Loss of BAX by miR-365 Promotes Cutaneous Squamous Cell Carcinoma Progression by Suppressing Apoptosis
Authors: Zhou Liang , Gao Ruirui , Wang Yinghui , Zhou Meijuan , Ding Zhenhua
Published: May 2017

Pro-apoptotic BCL2 associated X (BAX) is traditionally thought to be regulated by anti-apoptotic BCL-2 family members, like BCL2-like 1 (BCL-XL), at the protein level. However, the posttranscriptional regulation of BAX is under explored. In this study, we identified BAX as the novel downstream target of miR-365, which is supported by gain- and loss-of-function studies of onco-miR-365. Loss of BAX by either RNA interference or highly-expressed miR-365 in cells of cutaneous squamous cell carcinoma (CSCC) enhanced the tumor resistance against apoptosis, while repressing cell proliferation, migration, and invasiveness. In vivo experiment confirmed that BAX knockdown promotes the growth of CSCC xenografts. Collectively, our results find a miR-365-BAX axis for alleviating the pro-apoptotic effects of BAX, which promotes CSCC development and may facilitate the generation of novel therapeutic regimens to the clinical treatment of CSCC.
Linear Stability Prediction of Vortex Structures on High Pressure Turbine Blades
Authors: Zauner Markus , Sandham Neil , Wheeler Andrew , Sandberg Richard
Published: May 2017

Velocity profiles are extracted from time- and span-averaged direct numerical simulation data, describing the flow over a high-pressure turbine vane linear cascade near engine-scale conditions with reduced inlet disturbance levels. Based on these velocity profiles, local as well as non-local linear stability analysis of the boundary-layer over the suction side of the vane is carried out in order to characterise a linearly unstable region close to the trailing edge. The largest growth rates are found for oblique modes, but those are only slightly more unstable than 2D modes, which describe the locations and frequencies of most unstable modes very well. The frequencies of the most unstable linear modes predict with good accuracy the predominant frequencies found in the direct numerical simulations (DNS) close to the trailing edge.
Global Adaptation Controlled by an Interactive Consistency Protocol
Authors: Lenz Alina , Obermaisser Roman
Published: May 2017

Static schedules for systems can lead to an inefficient usage of the resources, because the system’s behavior cannot be adapted at runtime. To improve the runtime system performance in current time-triggered Multi-Processor System on Chip (MPSoC), a dynamic reaction to events is performed locally on the cores. The effects of this optimization can be increased by coordinating the changes globally. To perform such global changes, a consistent view on the system state is needed, on which to base the adaptation decisions. This paper proposes such an interactive consistency protocol with low impact on the system w.r.t. latency and overhead. We show that an energy optimizing adaptation controlled by the protocol can enable a system to save up to 43% compared to a system without adaptation.
Architectural Techniques for Improving the Power Consumption of NoC-Based CMPs: A Case Study of Cache and Network Layer
Authors: Ofori-Attah Emmanuel , Bhebhe Washington , Agyeman Michael
Published: May 2017

The disparity between memory and CPU have been ameliorated by the introduction of Network-on-Chip-based Chip-Multiprocessors (NoC-based CMPS). However, power consumption continues to be an aggressive stumbling block halting the progress of technology. Miniaturized transistors invoke many-core integration at the cost of high power consumption caused by the components in NoC-based CMPs; particularly caches and routers. If NoC-based CMPs are to be standardised as the future of technology design, it is imperative that the power demands of its components are optimized. Much research effort has been put into finding techniques that can improve the power efficiency for both cache and router architectures. This work presents a survey of power-saving techniques for efficient NoC designs with a focus on the cache and router components, such as the buffer and crossbar. Nonetheless, the aim of this work is to compile a quick reference guide of power-saving techniques for engineers and researchers.
Influence of the Constitutive Model for Shotcrete on the Predicted Structural Behavior of the Shotcrete Shell of a Deep Tunnel
Authors: Neuner Matthias , Schreter Magdalena , Unteregger David , Hofstetter Günter
Published: May 2017

The aim of the present paper is to investigate the influence of the constitutive model for shotcrete on the predicted displacements and stresses in shotcrete shells of deep tunnels. Previously proposed shotcrete models as well as a new extended damage plasticity model for shotcrete are evaluated in the context of 2D finite element simulations of the excavation of a stretch of a deep tunnel by means of the New Austrian Tunneling Method. Thereby, the behavior of the surrounding rock mass is described by the commonly used Hoek–Brown model. Differences in predicted evolutions of displacements and stresses in the shotcrete shell, resulting from the different shotcrete models, are discussed and simulation results are compared to available in situ measurement data.
Carbon-Supported Pd and PdFe Alloy Catalysts for Direct Methanol Fuel Cell Cathodes
Authors: Rivera Gavidia Luis , Sebastián David , Pastor Elena , Aricò Antonino , Baglio Vincenzo
Published: May 2017

Direct methanol fuel cells (DMFCs) are electrochemical devices that efficiently produce electricity and are characterized by a large flexibility for portable applications and high energy density. Methanol crossover is one of the main obstacles for DMFC commercialization, forcing the search for highly electro-active and methanol tolerant cathodes. In the present work, carbon-supported Pd and PdFe catalysts were synthesized using a sodium borohydride reduction method and physico-chemically characterized using transmission electron microscopy (TEM) and X-ray techniques such as photoelectron spectroscopy (XPS), diffraction (XRD) and energy dispersive spectroscopy (EDX). The catalysts were investigated as DMFC cathodes operating at different methanol concentrations (up to 10 M) and temperatures (60 °C and 90 °C). The cell based on PdFe/C cathode presented the best performance, achieving a maximum power density of 37.5 mW·cm−2 at 90 °C with 10 M methanol, higher than supported Pd and Pt commercial catalysts, demonstrating that Fe addition yields structural changes to Pd crystal lattice that reduce the crossover effects in DMFC operation.
Photoelectric Properties of Si Doping Superlattice Structure on 6H-SiC(0001)
Authors: Li Lianbi , Zang Yuan , Hu Jichao , Lin Shenghuang , Chen Zhiming
Published: May 2017

The energy-band structure and visible photoelectric properties of a p/n-Si doping superlattice structure (DSL) on 6H-SiC were simulated by Silvaco-TCAD. The,n the Si-DSL structures with 40 nm-p-Si/50 nm-n-Si multilayers were successfully prepared on 6H-SiC(0001) Si-face by chemical vapor deposition. TEM characterizations of the p/n-Si DSL confirmed the epitaxial growth of the Si films with preferred orientation and the misfit dislocations with a Burgers vector of 1/3 <21-1> at the p-Si/n-Si interface. The device had an obvious rectifying behavior, and the turn-on voltage was about 1.2 V. Under the visible illumination of 0.6 W/cm2, the device demonstrated a significant photoelectric response with a photocurrent density of 2.1 mA/cm2. Visible light operation of the Si-DSL/6H-SiC heterostructure was realized for the first time.
The Effect of Modulation Ratio of Cu/Ni Multilayer Films on the Fretting Damage Behaviour of Ti-811 Titanium Alloy
Authors: Zhang Xiaohua , Liu Daoxin , Li Xiaoying , Dong Hanshan , Xi Yuntao
Published: May 2017

To improve the fretting damage (fretting wear and fretting fatigue) resistance of Ti-811 titanium alloy, three Cu/Ni multilayer films with the same modulation period thickness (200 nm) and different modulation ratios (3:1, 1:1, 1:3) were deposited on the surface of the alloy via ion-assisted magnetron sputtering deposition (IAD). The bonding strength, micro-hardness, and toughness of the films were evaluated, and the effect of the modulation ratio on the room-temperature fretting wear (FW) and fretting fatigue (FF) resistance of the alloy was determined. The results indicated that the IAD technique can be successfully used to prepare Cu/Ni multilayer films, with high bonding strength, low-friction, and good toughness, which yield improved room-temperature FF and FW resistance of the alloy. For the same modulation period (200 nm), the micro-hardness, friction, and FW resistance of the coated alloy increased, decreased, and improved, respectively, with increasing modulation ratio of the Ni-to-Cu layer thickness. However, the FF resistance of the coated alloy increased non-monotonically with the increasing modulation ratio. Among the three Cu/Ni multilayer films, those with a modulation ratio of 1:1 can confer the highest FF resistance to the Ti-811 alloy, owing mainly to their unique combination of good toughness, high strength, and low-friction.
Influence of Electrical and Ionic Conductivities of Organic Electronic Ion Pump on Acetylcholine Exchange Performance
Authors: Abdullayeva Nazrin , Sankir Mehmet
Published: May 2017

By using an easy and effective method of depositing conjugated polymers (PEDOT:PSS) on flexible substrates, a new design for organic bioelectronic devices has been developed. The purpose was to build up a system that mimics the motion of neurotransmitters in the synaptic cleft by obtaining an electrical to chemical signal transport. Fourier transform infrared (FTIR) spectroscopy and Raman measurements have demonstrated that electrochemical overoxidation region which separates the pristine PEDOT:PSS electrodes and allows ionic conduction has been achieved successfully. The influence of both electrical and ionic conductivities on organic electronic ion pump (OEIP) performances has been studied. The ultimate goal was to achieve the highest equilibrium current density at the lowest applied voltage via enhancing the electrical conductivity of PEDOT:PSS and ionic conductivity of electrochemically overoxidized region. The highest equilibrium current density, which corresponds to 4.81 × 1017 number of ions of acetylcholine was about 41 μA cm−2 observed for the OEIP with the electrical conductivities of 54 S cm−1. This was a threshold electrical conductivity beyond which the OEIP performances were not changed much. Once Nafion™ has been applied for enhancing the ionic conductivity, the equilibrium current density increased about ten times and reached up to 408 μA cm−2. Therefore, it has been demonstrated that the OEIP performance mainly scales with the ionic conductivity. A straightforward method of producing organic bioelectronics is proposed here may provide a clue for their effortless mass production in the near future.
Investigation on Characteristic Variation of the FBG Spectrum with Crack Propagation in Aluminum Plate Structures
Authors: Jin Bo , Zhang Weifang , Zhang Meng , Ren Feifei , Dai Wei , Wang Yanrong
Published: May 2017

In order to monitor the crack tip propagation of aluminum alloy, this study investigates the variation of the spectrum characteristics of a fiber Bragg grating (FBG), combined with an analysis of the spectrum simulation. The results identify the location of the subordinate peak as significantly associated with the strain distribution along the grating, corresponding to the different plastic zones ahead of the crack tip with various crack lengths. FBG sensors could observe monotonic and cyclic plastic zones ahead of the crack tip, with the quadratic strain distribution along the grating at the crack tip-FBG distance of 1.2 and 0.7 mm, respectively. FBG sensors could examine the process zones ahead of the crack tip with the cubic strain distribution along the grating at the crack tip-FBG distance of 0.5 mm. The spectrum oscillation occurs as the crack approaches the FBG where the highly heterogeneous strain is distributed. Another idea is to use a finite element method (FEM), together with a T-matrix method, to analyze the reflection intensity spectra of FBG sensors for various crack sizes. The described crack propagation detection system may apply in structural health monitoring.
The Effects of Relative Humidity on the Flowability and Dispersion Performance of Lactose Mixtures
Authors: Lu Xiang-Yun , Chen Lan , Wu Chuan-Yu , Chan Hak-Kim , Freeman Tim
Published: May 2017

The flowability and dispersion behavior are two important physicochemical properties of pharmaceutical formulations for dry powder inhalers (DPIs). They are usually affected by the environmental conditions, such as temperature and relative humidity (RH). However, very few studies have been focused on the relationship between the two properties and their dependence on RH during storage. In this research, model pharmaceutical formulations were prepared using mixtures of coarse and fine lactose. The fractions of fines in the mixtures were 0%, 5%, 10%, and 20%, respectively. These blends were stored at four different RH levels, 0%, 30%, 58%, and 85%, for 48 h. The FT4 Powder Rheometer was used to evaluate the powder flowability, and the Malvern Spraytec® laser diffraction system was employed to assess the powder dispersion performance. The results indicated that both the flow and dispersion properties of lactose blends deteriorate after being stored at 85% RH, but improved after being conditioned at 58% RH. The fine particle fractions (FPFs) of the blends with 5% and 10% fine fractions and the as-received coarse lactose decreased when they were conditioned at 30% RH. For the blend with 20% fine fraction, a high RH during storage (i.e., 85% RH) affected the dispersion property, but had a limited influence on its flowability, while, for the coarse lactose powder, the different RH conditions affected its flowability, but not the dispersion results. A strong correlation between the powder flowability and its dispersion performance was found.
Bioactive Diphenyl Ethers and Isocoumarin Derivatives from a Gorgonian-Derived Fungus Phoma sp. (TA07-1)
Authors: Shi Ting , Qi Jun , Shao Chang-Lun , Zhao Dong-Lin , Hou Xue-Mei , Wang Chang-Yun
Published: May 2017

Three new diphenyl ether derivatives—phomaethers A–C (1–3) and five known compounds—including a diphenyl ether analog, 2,3′-dihydroxy-4-methoxy-5′,6-dimethyl diphenyl ether (4); and four isocoumarin derivatives, diaportinol (5), desmethyldiaportinol (6), citreoisocoumarinol (7), and citreoisocoumarin (8)—were isolated from a gorgonian-derived fungus Phoma sp. (TA07-1). Their structures were elucidated by extensive spectroscopic investigation. The absolute configurations of 1 and 2 were determined by acid hydrolysis reactions. It was the first report to discover the diphenyl glycoside derivatives from coral-derived fungi. Compounds 1, 3, and 4 showed selective strong antibacterial activity against five pathogenic bacteria with the minimum inhibiting concentration (MIC) values and minimum bactericidal concentration (MBC) values between 0.156 and 10.0 μM.