bluuebunny/crossref_metadata_2025 huggingface dataset:
Variability in physical inactivity responses of university students during COVID-19 pandemic: A monitoring of daily step counts using a smartphone application
Authors: Konda Shoji , Ogasawara Issei , Fujita Kazuki , Aoyama Chisa , Yokoyama Teruki , Magome Takuya , Yulong Chen , Hashizume Ken , Matsuo Tomoyuki , Nakata Ken
Published: December 2021

This study investigated the changes in physical inactivity of university students during the COVID-19 pandemic, with reference to their academic calendar. We used the daily step counts recorded by a smartphone application (iPhone Health App) from April 2020 to January 2021 (287 days) for 603 students. The data for 287 days were divided into five periods based on their academic calendar. The median value of daily step counts across each period was calculated. A k-means clustering analysis was performed to classify the 603 participants into subgroups to demonstrate the variability in the physical inactivity responses. The median daily step counts, with a 7-days moving average, dramatically decreased from 5,000 to 2,000 steps/day in early April. It remained at a lower level (less than 2,000 steps/day) during the first semester, then increased to more than 5,000 steps/day at the start of summer vacation. The clustering analysis demonstrated the variability in physical inactivity responses. Independent of the academic calendar, many inactive students did not recover their original daily step counts after its dramatic decrement. Consequently, promoting physical activity is recommended for inactive university students over the course of the whole semester.
Genetic and transcriptomic characteristics of RhlR-dependent quorum sensing in cystic fibrosis isolates of Pseudomonas aeruginosa
Authors: Asfahl Kyle L. , Smalley Nicole E. , Chang Alexandria , Dandekar Ajai A.
Published: December 2021

In people with the genetic disease cystic fibrosis (CF), bacterial infections involving the opportunistic pathogen Pseudomonas aeruginosa are a significant cause of morbidity and mortality. P. aeruginosa uses a cell-cell signaling mechanism called quorum sensing (QS) to regulate many virulence functions. One type of QS consists of acyl-homoserine lactone (AHL) signals produced by LuxI-type signal synthases, which bind a cognate LuxR-type transcription factor. In laboratory strains and conditions, P. aeruginosa employs two AHL synthase/receptor pairs arranged in a hierarchy, with the LasI/R system controlling the RhlI/R system and many downstream virulence factors. However, P. aeruginosa isolates with inactivating mutations in lasR are frequently isolated from chronic CF infections. We and others have shown that these isolates frequently use RhlR as the primary QS regulator. RhlR is rarely mutated in CF and environmental settings. We were interested if there were reproducible genetic characteristics of these isolates and if there was a central group of genes regulated by RhlR in all isolates. We examined five isolates and found signatures of adaptation common to CF isolates. We did not identify a common genetic mechanism to explain the switch from Las-to Rhl-dominated QS. We describe a core RhlR regulon encompassing 20 genes encoding 7 products. These results suggest a key group of QS-regulated factors important for pathogenesis of chronic infection, and position RhlR as a target for anti-QS therapeutics. Our work underscores the need to sample a diversity of isolates to understanding QS beyond what has been described in laboratory strains.
An Atypical RNA Quadruplex Marks RNAs as Vectors for Gene Silencing
Authors: Roschdi Saeed , Yan Jenny , Nomura Yuichiro , Escobar Cristian A , Petersen Riley J , Bingman Craig A , Tonelli Marco , Montemayor Eric J , Kennedy Scott , BUTCHER SAMUEL
Published: December 2021

The addition of poly(UG) ("pUG") repeats to 3' termini of mRNAs drives gene silencing and trans-generational epigenetic inheritance in the metazoan C. elegans. pUG tails promote silencing by recruiting an RNA-dependent RNA Polymerase (RdRP) that synthesizes small interfering (si)RNAs. Here we show that active pUG tails require a minimum of 11.5 repeats and adopt a quadruplex (G4)2 structure we term the pUG fold. The pUG fold differs from known G4s in that it has a left-handed backbone similar to Z-RNA, no consecutive guanosines in its sequence, and three G quartets and one U quartet stacked non-sequentially. Its biological importance is emphasized by our observations that porphyrin molecules bind to the pUG fold and inhibit both gene silencing and binding of RdRP. Moreover, specific N7-deaza RNA substitutions that do not adopt the pUG fold neither bind RdRP nor induce RNA silencing. These data define the pUG fold as a previously unrecognized RNA secondary structure motif that drives gene silencing. The pUG fold can also form internally within larger RNA molecules. Approximately 20,000 pUG-fold sequences are found in non-coding regions of human RNAs, suggesting the fold likely has biological roles beyond gene silencing.
Even well-practiced movements benefit from repetition
Authors: Sutter Katrin , Wijdenes Leonie Oostwoud , van Beers Robert J. , Medendorp W. Pieter
Published: December 2021

Professional golf players spend years practicing, but will still perform one or two practice swings without a ball before executing the actual swing. Why do they do this? In this study we tested the hypothesis that repeating a well-practiced movement leads to a reduction of movement variability. To operationalize this hypothesis, participants were tested in a center- out reaching task with four different targets, on four different days. To probe the effect of repetition they performed random sequences from one to six movements to the same target. Our findings show that, with repetition, movements are not only initiated earlier but their variability is reduced across the entire movement trajectory. Furthermore, this effect is present within and across the four sessions. Together, our results suggest that movement repetition changes the tradeoff between movement initiation and movement precision.

New & Noteworthy

Professional athletes practice movements that they have performed thousands of times in training just before it is their turn in a game. Why do they do this? Our results indicate that both initial and endpoint variability reduce with repetition in a short sequence of reaching movements. This means that even well-practiced movements benefit from practice.
Replication is the key barrier during the dual-host adaption of mosquito-borne flaviviruses
Authors: Zhang Yanan , Liang Dening , Yuan Fei , Yan Yiran , Wang Zuoshu , Liu Pan , Yu Qi , Zhang Xing , Wang Xiangxi , Zheng Aihua
Published: December 2021

Mosquito-borne flaviviruses (MBFs) adapt to a dual-host transmission circle between mosquitoes and vertebrates. Dual-host affiliated insect-specific flaviviruses (dISFs), discovered from mosquitoes, are phylogenetically similar to MBFs but do not infect vertebrates. Thus, dISF-MBF chimeras could be an ideal model to study the dual-host adaption of MBFs. Using the pseudo-infectious reporter virus particle and reverse genetics systems, we found dISFs entered vertebrate cells as efficiently as the MBFs, but failed to initiate replication. Exchange of the un-translational regions (UTRs) of Donggang virus (DONV), an dISF, with those from Zika virus (ZIKV) rescued DONV replication in vertebrate cells and critical secondary RNA structures were further mapped. Essential UTR-binding host factors were screened for ZIKV replication in vertebrate cells, displaying different binding patterns. Therefore, our data demonstrate a post-entry cross-species transmission mechanism of MBFs, while UTR-host interaction is critical for dual-host adaption.

Significance Statement

Most viruses have a relatively narrow host range. In contrast, vector-borne flaviviruses, such as dengue virus and Zika virus, maintain their transmission cycle between arthropods and vertebrates, belonging to different phyla. How do these viruses adapt to the distinct cellular environments of two phyla? By comparing the single-host insect specific flavivirus and dual-host Zika virus, we identified three key molecular factors that determine MBF host tropism. This study will greatly increase the understanding of entry, replication, and cross-species evolution of mosquito-borne flaviviruses.
Integrated metabolomic, molecular networking, and genome mining analyses uncover novel angucyclines from Streptomyces sp. RO-S4 strain isolated from Bejaia Bay, Algeria
Authors: Ouchene Rima , Stien Didier , Segret Juliette , Kecha Mouloud , Rodrigues Alice M. S. , Veckerlé Carole , Suzuki Marcelino T.
Published: December 2021

Multi-omic approaches have recently made big strides towards the effective exploration of microorganisms and accelerating the discovery of new bioactive compounds. We combined metabolomic, molecular networking, and genomic-based approaches to investigate the metabolic potential of the Streptomyces sp. RO-S4 strain isolated from the polluted waters of Bejaia Bay in Algeria. Antagonistic assays against methicillin-resistant Staphylococcus aureus with RO-S4 organic extracts showed an inhibition zone of 20 mm by the agar diffusion method, and its minimum inhibitory concentration was 16 μg/mL. A molecular network was created using GNPS and annotated through the comparison of MS/MS spectra against several databases. The predominant compounds in the RO-S4 extract belonged to the angucyclines family. Three compounds were annotated as known metabolites, while all the others were putatively new to Science. Notably, all compounds had fridamycin-like aglycones, and several of them had a lactonized D ring analogous to that of urdamycin L. The whole genome of Streptomyces RO-S4 was sequenced to identify the biosynthetic gene cluster (BGC) encoding for these angucyclines, which yielded a draft genome of 7,497,846 bp with 72.4% G+C content. Subsequently, a genome mining analysis revealed 19 putative biosynthetic gene clusters, including a grincamycin-like BGC with a high similarity to that of Streptomyces sp. CZN-748 previously reported to also produce mostly open fridamycin-like aglycones. As the ring-opening process leading to these compounds is still not defined, we performed comparative analysis with other angucycline BGCs and advanced some hypotheses to explain the ring-opening and lactone formation, possibly linked to the uncoupling between the activity of GcnE and GcnM homologues in the RO-S4 strain. The combination of metabolomic and genomic approaches greatly improved the interpretation of the metabolic potential of the RO-S4 strain.
Gustatory cortex is involved in evidence accumulation during food choice
Authors: Ataei Ali , Amini Arash , Ghazizadeh Ali
Published: December 2021

Food choice is one of the most fundamental and most frequent value-based decisions for all animals including humans. However, the neural circuitry involved in food-based decisions is only recently being addressed. Given the relatively fast dynamics of decision formation, EEG-informed fMRI analysis is highly beneficial for localizing this circuitry in humans. Here by using the EEG correlates of evidence accumulation in a simultaneously recorded EEG-fMRI dataset, we found a significant role for the right temporal-parietal operculum (PO) and medial insula including gustatory cortex (GC) in binary choice between food items. These activations were uncovered by using the “EEG energy” (power 2) as the BOLD regressor and were missed if conventional analysis with the EEG signal itself were to be used, in agreement with theoretical predictions for EEG and BOLD relations. No significant positive correlations were found with higher powers of EEG (powers 3 or 4) pointing to specificity and sufficiency of EEG energy as the main correlate of the BOLD response. This finding extends the role of cortical areas traditionally involved in palatability processing to value-based decision making and offers the “EEG energy” as a key regressor of BOLD response in simultaneous EEG-fMRI designs.
Suicidal chemotaxis in bacteria
Authors: Oliveira Nuno M. , Wheeler James H. R. , Deroy Cyril , Booth Sean C. , Walsh Edmond J. , Durham William M. , Foster Kevin R.
Published: December 2021

Bacteria commonly live in communities on surfaces where steep gradients of antibiotics and other chemical compounds routinely occur. While many species of bacteria can move on surfaces, we know surprisingly little about how such antibiotic gradients affect cell motility. Here we study the behaviour of the opportunistic pathogen Pseudomonas aeruginosa in stable spatial gradients of a range of antibiotics by tracking thousands of cells in microfluidic devices as they form biofilms. Unexpectedly, these experiments reveal that individual bacteria use pili-based (‘twitching’) motility to actively navigate towards regions with higher antibiotic concentrations. Our analyses suggest that this biased migration is driven, at least in part, by a direct response to the antibiotics. Migrating cells can reach antibiotic concentrations hundreds of times higher than their minimum inhibitory concentration in a few hours and remain highly motile. However, isolating these cells - using fluid-walled microfluidic devices that can be reconfigured in situ - suggests that these bacteria are terminal and not able to reproduce. In spite of moving towards their death, we show that migrating cells are capable of entering a suicidal program to release bacteriocins that are used to kill other bacteria. Our work suggests that bacteria respond to antibiotics as if they come from a competing colony growing in the neighbourhood, inducing them to invade and attack. As a result, clinical antibiotics have the potential to serve as a bait that lures bacteria to their death.
The marine gastropod Conomurex luhuanus (Strombidae) has high-resolution spatial vision and eyes with complex retinas
Authors: Irwin Alison R. , Williams Suzanne T. , Speiser Daniel I. , Roberts Nicholas W.
Published: December 2021

All species within the conch snail family Strombidae possess large camera-type eyes that are surprisingly well-developed compared to those found in most other gastropods. Although these eyes are known to be structurally complex, very little research on their visual function has been conducted. Here, we use isoluminant expanding visual stimuli to measure the spatial resolution and contrast sensitivity of a strombid, Conomurex luhuanus. Using these stimuli, we show that this species responds to objects as small as 1.06° in its visual field. We also show that C. luhuanus responds to Michelson contrasts of 0.07, a low contrast sensitivity between object and background. The defensive withdrawal response elicited by visual stimuli of such small angular size and low contrast suggests that conch snails may use spatial vision for the early detection of potential predators. We support these findings with morphological estimations of spatial resolution of 1.04 ± 0.14°. These anatomical data therefore agree with the behavioural measures and highlight the benefits of integrating morphological and behavioural approaches in animal vision studies. Furthermore, using contemporary imaging techniques including serial block-face scanning electron microscopy (SBF-SEM), in conjunction with transmission electron microscopy (TEM), we found that C. luhuanus have more complex retinas, in terms of cell type diversity, than previous studies of the group have discovered using TEM alone. We found the C. luhuanus retina is comprised of six cell types, including a newly identified ganglion cell and accessory photoreceptor, rather than the previously described four cell types.

Summary statement

Behavioural trials indicate the eyes of conch snail species Conomurex luhuanus provide high-resolution spatial vision, and morphological examination reveals the retina contains more cell types than those of other gastropods.
Probing the molecular mechanism of the action of Deinococcus radiodurans RecD2 at single-molecule resolution
Authors: Purkait Debayan , Islam Farhana , Mishra Padmaja P.
Published: December 2021

Helicases are ATP-driven molecular machines that directionally remodel nucleic acid polymers in all three domains of life. Helicases are responsible for resolving double-stranded DNA (dsDNA) into separate single-strands and this activity is essential for DNA replication, nucleotide excision repair, and homologous recombination. RecD2 from Deinococcus radiodurans (DrRecD2) has important contributions towards the organism’s unusually high tolerance to gamma radiation and hydrogen peroxide. Although previous X-ray Crystallography studies have revealed the structural characteristics of the protein, the direct experimental evidence regarding the dynamics of the DNA unwinding process by DrRecD2 in the context of other accessory proteins is yet to be found. In this study, we have probed the exact binding event and processivity of DrRecD2 at single-molecule resolution using Protein-induced fluorescence enhancement (smPIFE) and Forster resonance energy transfer (smFRET). We have found that the protein prefers to bind at the 5 ‘ terminal end of the single-stranded DNA (ssDNA) by Drift and has helicase activity even in absence of ATP. However, a faster and iterative mode of DNA unwinding was evident in presence of ATP. The rate of translocation of the protein was found to be slower on dsDNA compared to ssDNA. We also showed that DrRecD2 is recruited at the binding site by the single-strand binding protein (SSB) and during the unwinding, it can displace RecA from ssDNA.
Metabarcoding malaise trap plant components enables monitoring the diversity of plant-insect interactions
Authors: Swenson Stephanie J. , Eichler Lisa , Hörren Thomas , Lehmann Gerlind U. C. , Sorg Martin , Gemeinholzer Birgit
Published: December 2021

The declines observed in insect abundance and diversity in the past decades has also been observed in plants, and these events are most certainly correlated. Rapid largescale biomonitoring of both plants and insects can help monitor these changes and inform decisions for land management and species protection. Malaise traps have been used for nearly 80 years for passive insect sampling of primarily flying insects, and when they enter these traps, they carry the fragments of the plants they have visited, either as plant fragments and pollen on the body surface, or as digested food material in gut contents. DNA metabarcoding is a potential method to identify these plant traces in the ethanol of the malaise bottles, which is not possible with traditional microscopy. Metabarcoding could offer more insight into what plants insects are directly interacting with at a given time, and allow for the detection of rare plants, and neophyte species visited by insects. This study, to our knowledge, is the first examination of DNA metabarcoding plant traces from Malaise trap samples, we examine 105 samples from 21 sites throughout Germany collected in a 2-week period in May of 2020. Here we report on the feasibility of sequencing these sample types, analysis of the resulting taxa, the usage of cultivated plants by insects near nature conservancy areas, and the detection of rare and neophyte species.
Assessing the utility of marine filter feeders for environmental DNA (eDNA) biodiversity monitoring
Authors: Jeunen Gert-Jan , Cane Jasmine S. , Ferreira Sara , Strano Francesca , von Ammon Ulla , Cross Hugh , Day Robert , Hesseltine Sean , Ellis Kaleb , Urban Lara , Pearson Niall , Olmedo-Rojas Pamela , Kardailsky Anya , Gemmell Neil J. , Lamare Miles
Published: December 2021

Aquatic environmental DNA (eDNA) surveys are transforming how we monitor marine ecosystems. The time-consuming pre-processing step of active filtration, however, remains a bottleneck. Hence, new approaches omitting active filtration are in great demand. One exciting prospect is to use the filtering power of invertebrates to collect eDNA. While proof-of-concept has been achieved, comparative studies between aquatic and filter feeder eDNA signals are lacking. Here, we investigated the differences among four eDNA sources (water; bivalves; sponges; and ethanol in which filter-feeding organisms were stored) along a vertical transect in Doubtful Sound, New Zealand using three metabarcoding primers (fish (16S); MiFish-E/U). While concurrent SCUBA diver observations validated eDNA results, laboratory trials corroborated in-field bivalve eDNA detection results. Combined, eDNA sources detected 59 vertebrates, while divers observed eight fish species. There were no significant differences in alpha and beta diversity between water and sponge eDNA and both sources were highly correlated. Vertebrate eDNA was detected in ethanol, although only a reduced number of species were detected. Bivalves failed to reliably detect eDNA in both field and mesocosm experiments. While additional research into filter feeder eDNA accumulation efficiency is essential, our results provide strong evidence for the potential of incorporating sponges into eDNA surveys.
Mediator is broadly recruited to gene promoters via a Tail-independent mechanism
Authors: Warfield Linda , Donczew Rafal , Mahendrawada Lakshmi , Hahn Steven
Published: December 2021

Mediator (MED) is a conserved factor with important roles in both basal and activated transcription. It is believed that MED plays a direct role in transcriptional regulation at most genes by functionally bridging enhancers and promoters. Here, we investigate the genome-wide roles of yeast MED by rapid depletion of its activator-binding domain (Tail) and monitoring changes in nascent transcription. We find that MED Tail and activator-mediated MED recruitment regulate only a small subset of genes. At most genes, MED bypasses the UAS and is directly recruited to promoters to facilitate transcription initiation. Our results define three classes of genes that differ in PIC assembly pathways and the requirements for MED Tail, SAGA, TFIID and BET factors Bdf1/2. We also find that the depletion of the MED middle module subunit Med7 mimics inactivation of Tail, suggesting a functional link. Our combined results have broad implications for the roles of MED, other coactivators, and mechanisms of transcriptional regulation at different gene classes.
Tracing electron density changes in langbeinite under pressure
Authors: Gajda Roman , Zhang Dongzhou , Parafiniuk Jan , Dera Przemysław , Woźniak Krzysztof
Published: December 2021

Pressure is well known to dramatically alter physical properties and chemical behaviour of materials, much of which is due to the changes in chemical bonding that accompany compression. Though it is relatively easy to comprehend this correlation in the discontinuous compression regime, where phase transformations take place, understanding of the more subtle continuous compression effects is a far greater challenge, requiring insight into the finest details of electron density redistribution. In this study, a detailed examination of quantitative electron density redistribution in the mineral langbeinite was conducted at high pressure. Langbeinite is a potassium magnesium sulfate mineral with the chemical formula [K2Mg2(SO4)3], and crystallizes in the isometric tetartoidal (cubic) system. The mineral is an ore of potassium, occurs in marine evaporite deposits in association with carnallite, halite and sylvite, and gives its name to the langbeinites, a family of substances with the same cubic structure, a tetrahedral anion, and large and small cations. Single-crystal X-ray diffraction data for langbeinite have been collected at ambient pressure and at 1 GPa using a combination of in-house and synchrotron techniques. Experiments were complemented by theoretical calculations within the pressure range up to 40 GPa. On the basis of changes in structural and thermal parameters, all ions in the langbeinite structure can be grouped into `soft' (potassium cations and oxygens) and `hard' (sulfur and magnesium). This analysis emphasizes the importance of atomic basins as a convenient tool to analyse the redistribution of electron density under external stimuli such as pressure or temperature. Gradual reduction of completeness of experimental data accompanying compression did not significantly reduce the quality of structural, electronic and thermal parameters obtained in experimental quantitative charge density analysis.
On the frequency module of the hull of a primitive substitution tiling
Authors: Say-awen April Lynne D. , Frettlöh Dirk , De Las Peñas Ma. Louise Antonette N.
Published: December 2021

Understanding the properties of tilings is of increasing relevance to the study of aperiodic tilings and tiling spaces. This work considers the statistical properties of the hull of a primitive substitution tiling, where the hull is the family of all substitution tilings with respect to the substitution. A method is presented on how to arrive at the frequency module of the hull of a primitive substitution tiling (the minimal {\bb Z}-module, where {\bb Z} is the set of integers) containing the absolute frequency of each of its patches. The method involves deriving the tiling's edge types and vertex stars; in the process, a new substitution is introduced on a reconstructed set of prototiles.
B-factor accuracy in protein crystal structures
Authors: Carugo Oliviero
Published: December 2021

The accuracy of B factors in protein crystal structures has been determined by comparing the same atoms in numerous, independent crystal structures of Gallus gallus lysozyme. Both B-factor absolute differences and normal probability plots indicate that the estimated B-factor errors are quite large, close to 9 Å2 in ambient-temperature structures and to 6 Å2 in low-temperature structures, and surprisingly are comparable to values estimated two decades ago. It is well known that B factors are not due to local movements only but reflect several, additional factors from crystal defects, large-scale disorder, diffraction data quality etc. It therefore remains essential to normalize B factors when comparing different crystal structures, although it has clearly been shown that they provide useful information about protein dynamics. Improved, quantitative analyses of raw B factors require novel experimental and computational tools that are able to disaggregate local movements from other features and properties that affect B factors.
Corporate board structures and intellectual capital: evidence from banks in Africa
Authors: Asare Nicholas , Aboagye-Otchere Francis , Onumah Joseph Mensah
Published: December 2021

Purpose

This study examines the nature of the relationship between board structures (BSs) and intellectual capital (IC) of banks in Africa.

Design/methodology/approach

Using annual data from financial statements of 366 banks from 26 African countries from 2007 to 2015, the study estimates IC using the value-added intellectual coefficient (VAIC) and BSs using board size, board independence and board gender diversity. The system generalized method of moments and panel-corrected standard error estimation strategies are used to estimate panel regressions.

Findings

There is a significant negative relationship between board independence and intellectual capital. The results also indicate that the IC of banks does not depend on board size and board gender diversity.

Practical implications

The study's findings provide evidence of the extent to which BSs have been instituted to support investments in intellectual capital as a means of improving the performance of banks in Africa.

Originality/value

This study provides some empirical evidence from Africa's banking sector to justify that banks with better IC have boards that are less independent. This study is one of the few studies that employs many countries' data.
Board diversity and financial performance: empirical evidence from the United Kingdom
Authors: Hosny Khaled , Elgharbawy Adel
Published: December 2021

Purpose

This study aims to investigate the relationship between board diversity and financial performance from a wide perspective, including multiple dimensions of board diversity.

Design/methodology/approach

The cross-sectional design of the FTSE 350 companies in the period of 2013–2019 was adopted in this study. Data were collected using the Thomson Reuters Eikon and BoardEx databases and analyzed via ordinary least Squares (OLS) regression.

Findings

Both gender and skill diversity positively affect financial performance. However, other dimensions of diversity, including board tenure, education and network, have no significant influence on financial performance. On the other hand, nationality diversity negatively affects financial performance, and the gender diversity of executive directors negatively affects market-based performance. The results remain unchanged after considering endogeneity concerns and using alternative measures of financial performance.

Practical implications

This study provides useful insights into the importance of board diversity and its implications for firm performance, which can help in the development of future regulations and policies, such as female representation on the board. The findings can also guide companies toward the best way of diversifying their boardrooms in different aspects.

Originality/value

This study extensively investigates board diversity, including gender, tenure, skill and education, network and nationality, using the lens of the resource dependency theory. It also extends the scope of the study to examine some characteristics of executive directors, including gender and age. The evidence is provided from one of the leading countries in regulating corporate governance (CG), i.e. the UK.
Virtual water flows and economic water productivity of Italian fair-trade: the case of bananas, cocoa and coffee
Authors: Miglietta Pier Paolo , Fischer Christian , De Leo Federica
Published: December 2021

Purpose

In a globalized economic system, the role of agrifood production is to ensure at the same time both the population's livelihood and environmental resource conservation. The present study aims at expanding the debate on the potentials of the fair-trade in terms of environmental sustainability.

Design/methodology/approach

The research presents a methodology divided into three phases: (1) the identification of the water footprint values associated with the production of bananas, cocoa and coffee imported from developing countries to Italy; (2) the calculation of the virtual water volumes used to produce the crops imported from developing countries to Italy through fair-trade; (3) the analysis of the economic water productivity, obtained by the fair-trade premium, for bananas, cocoa and coffee.

Findings

The results of this study identified and measured the amount of virtual water flows and water savings or losses deriving from the fair-trade of bananas, cocoa and coffee. The average virtual water flow related to the fair-trade imports in Italy amounts to 7.27 million m3 for bananas, 22,275 m3 for cocoa and 14,334 m3 for coffee. The research findings also highlight that fair-trade and the related premium ensures at the same time the achievement of social and institutional purposes but also the remuneration of virtual water used within the life cycle of the imported crops.

Originality/value

Previous scientific literature showed that fair-trade premium has commonly been used to finance environmental protection. No study has evaluated the environmental impacts associated with fair-trade, nor the monetary value associated with the natural resources exploited to produce crops to be exported. This empirical paper fills a literature gap in terms of identification, measurement and evaluation of virtual water flows along the supply chain processes of some fair-traded crops, also providing, through the economic water productivity approach, a useful tool for decision-makers.
The optimization of spray-drying process for the development of apricot powder using response surface methodology
Authors: Bashir Omar , Hussain Syed Zameer , Amin Tawheed , Jan Nusrat , Gani Gousia , Bhat Shakeel Ahmad , Jabeen Abida
Published: December 2021

Purpose

Apricots are not only nutritionally-rich but also possess pharmacological significance owing to their high antioxidant activity, and they are rich in vitamins, fibers, bioactive phytochemicals and minerals. Because of its immense organoleptic characteristics, apricot juice (AJ) is well accepted; however, it has a limited shelf-life, thereby demanding it to be converted into other shelf-stable form. One of the approaches is converting this juice into dehydrated powder. Amongst the various dehydration techniques available, spray drying is usually preferred; however, it involves the use of several independent variables, which need to be optimized, thus prompting to optimize the process to obtain spray dried apricot powder (SDAP) with improved quality.

Design/methodology/approach

The spray-drying process of apricot juice was done using the response surface approach. The process variables included the inlet air temperature of 135–220°C, gum arabic concentration of 4–25%, feed flow rate of 124–730 mL/h, feed total soluble solids (TSS) of 10-30°Brix and atomization speed of 11,400–28,000 rpm. The dependent responses were powder yield, hygroscopicity, solubility, moisture content, carotenoids (CT), ascorbic acid (AA), radical scavenging activity (RSA), lightness, wettability, bulk density, particle density and porosity.

Findings

Amongst all independent variables, inlet air temperature had most predominant impact on all the investigated responses. The optimum processing conditions for development of apricot powder with optimum quality were 190°C inlet air temperature, 18.99% gum arabic, 300.05 mL/h feed flow rate, 24°Brix feed TSS and 17433.41 rpm atomization speed. The experimental values were found to be in agreement with the predicted values, indicating the suitability of models in predicting optimizing responses of apricot powder. Flowability as Carr's index (CI) (22.36 ± 1.01%) suggests fair flow of powder. Glass transition temperature of powder was 57.85 ± 2.03°C, which is much higher than that of ambient, suggesting its better shelf stability.

Originality/value

To the best of author's knowledge, very limited or very few studies have been carried out on the spray-drying process for the manufacture of SDAP. The results of this investigation will open up new horizons in the field of food industry in the Union Territory of Jammu and Kashmir, India or elsewhere in the apricot-growing areas of India.
Can earning much lead to doing good? Firm performance implications for corporate social responsibility
Authors: Hou Siyu , Guo Zhaoyang , Cai Chuangneng , Jiao Xiaobo
Published: December 2021

Purpose

The purpose of this study is to examine the influence of firm performance on corporate social responsibility (CSR) and its possible moderating effect. Despite the significance of CSR, there remains an extensive debate about how it is affected by firm performance.

Design/methodology/approach

The conceptual model is mainly built on goal-setting theory. Based on archival data from multiple data sets on 1,650 companies, collected from 2010 to 2017, the hypotheses are tested using the two-stage instrumental variable regression method.

Findings

There is an inverted U-shaped relationship between firm performance and CSR that first increases and then decreases. In addition, considering the boundary conditions, state ownership makes the inverted U-shaped curve steeper, while high executive wage concentration makes the inverted U-shaped curve flatter.

Research limitations/implications

This study harmonizes the traditional contradictory findings of the influence of firm performance on CSR, that is, it supports a positive, negative or neutral relationship between the two.

Originality/value

This research provides a necessary structure for the CSR literature. By delving deeply into the relationship between firm performance and CSR, it enables scholars to better address the critical management question of whether earning more will lead to doing good.
Participation of Indigenous employees in the Quebec's forestry sector: opportunities and barriers
Authors: Beaudoin Jean-Michel , Dufour Marie-Eve , Desroches-Maheux Eve , Lebel Luc
Published: December 2021

Purpose

The purpose of this study is to better understand the factors influencing the attraction of Indigenous workers to the Quebec forestry sector.

Design/methodology/approach

Using a collaborative approach, 64 semi-structured interviews were conducted between 2016 and 2018 with workers and stakeholders from three Indigenous communities in Quebec, Canada.

Findings

The results highlight the motivations for choosing a job in the forestry sector, including family and friends, attachment to the territory, financial necessity, the search for a challenge and a sense of pride. They also show some of the obstacles to holding a job in forestry, namely work–life conflict, transportation, job insecurity, education and personal problems.

Social implications

Indigenous people have a lower employment rate than non-Indigenous people, which can be explained by a number of factors that hinder their integration into the labour market. They nevertheless represent an interesting labour pool for companies working in the natural resources sector. This study sheds light on the opportunities and barriers to attract this workforce.

Originality/value

The study is one of the few to use theoretical frameworks focused on motivation and a qualitative approach to data collection in order to examine to examine the attraction of Indigenous workers to the forestry sector in Quebec (Canada) from a worker's perspective.
Environmental impact assessment of CPEC: a way forward for sustainable development
Authors: Khalid Iram , Ahmad Tooba , Ullah Sami
Published: December 2021

Purpose

Human-induced changes in climate have affected the environment to the extent that any more economic development at the cost of the environment will be too costly. Thus, sustainable development options posing no additional harm to the environment are the only viable option. This study aims to examine the likely environmental impacts of infrastructural developments through the China–Pakistan Economic Corridor (CPEC).

Design/methodology/approach

There is a scarcity of academic debate and discussion on the environmental impact of CPEC developments in laws and policies on the environment. The qualitative approach is followed in this study and official documents and reports are used to investigate the environmental challenges posed by CPEC.

Findings

The findings show three possible environmental concerns which could increase the climate change vulnerability of Pakistan. The coal-fired power plants are the most prominent threat based on their CO2 contributions and smog. Second, cutting more than 54,000 trees for roads infrastructure will increase CO2 concentration along the CPEC route. Third, increasing vehicle trafficking by up to 7,000 trucks per day on Karakorum Highway alone will release 36.5 million tons of additional CO2.

Originality/value

It is essential to rethink the environmental cost of CPEC. The study suggests economic and legal cooperation between Pakistan and China as a way forward to deal with climate change issues. Environmental laws should be a vital part of CPEC projects to ensure their safety, security and sustainability.
Memetics as informational difference: offering an information-centric conception of memes
Authors: Smith Alexander O. , Hemsley Jeff
Published: December 2021

Purpose

Information scientists may find value in studying cultural information evolution and information diffusion through memetics. Information studies in memetics have often found datafication in memetics research difficult. Meanwhile, current memetic scholarship elsewhere is abundant in data due to their focus on Internet artifacts. This paper offers a way to close the datafication gap for information researchers by associating information data with “differences” between memetic documents.

Design/methodology/approach

This work offers a joint theory and methodology invested in information-oriented memetics. This methodology of differences is developed from a content analysis of difference on a collection of images with visual similarities.

Findings

The authors find that this kind of analysis provides a heuristic method for quantitatively bounding where one meme ends and another begins. The authors also find that this approach helps describe the dynamics of memetic media in such a way that the authors can datafy information or cultural evolution more clearly.

Originality/value

Here the authors offer an approach for studying cultural information evolution through the study of memes. In doing so, the authors forward a methodology of difference which leverages content analysis in order to outline how it functions practically. The authors propose a quantitative methodology to assess differences between versions of document contents in order to examine what a particular meme is. The authors also move toward showing the information structure which defines a meme.
Product differentiation and populistic entry strategies in a mortgage market with Bertrand competition
Authors: Borgersen Trond Arne
Published: December 2021

Purpose

The purpose of this paper is to analyse the interaction between a profit maximising mortgagor and a newcomer to a mortgage market with Bertrand competition where the newcomer has a populistic entry strategy and undercuts mortgage market rates. The intention of the paper is to relate the populistic entry strategy to mortgage market characteristics and the strategic market position of both the established mortgagor and the newcomer in question.

Design/methodology/approach

The paper analyses a mortgage market by combining the behaviour of a profit maximising mortgagor with that of a newcomer to the mortgage market which has a populistic entry strategy and does not maximise profits. The short-run market solution provides comparative statics on the strategic market position of both the established mortgagor and the newcomer to the mortgage market during the entry phase both related to product differentiation and to price mirroring and undercutting of mortgage rates.

Findings

The model finds a mortgage market solution where a lower mortgage rate helps the newcomer gain a customer base. As the newcomer's strategy to mirror prices makes it unable to pass-through funding cost to its mortgage rate, the strategy is unsustainable over time. The established mortgagor has a strategically beneficial position as the mortgage market rates only relate to its funding cost. Unless the newcomer has a funding cost advantage, the established mortgagor has a higher interest rate margin. Differentiation impacts the newcomers’ interest rate margin positively. If the newcomer lacks a funding cost advantage, there is a critical mirroring rate that ensures it a higher interest rate margin. The higher the newcomers’ own funding cost, the higher is the upper bound for price mirroring, relating market entry to a small undercutting of mortgage rates and a mortgage market with weak competition. The funding cost of the established mortgagor pulls pricing in the opposite direction, allowing for a lower mirroring rate and tougher mortgage market competition during entry.

Originality/value

The paper aims to contribute to the understanding of market equilibrium in the absence of profit maximising behaviour. Framing a mortgage market in terms of a duopoly where a newcomer enters with a populistic entry strategy offering a lower mortgage rate and a mortgage product with a different loan-to-value (LTV) ratio, a novel mortgage market case comes about. The populistic entry strategy produces an augmented reaction curve, crucial for the mortgage market rates.
Effects of connected and autonomous vehicle merging behavior on mainline human-driven vehicle
Authors: Yue Lishengsa , Abdel-Aty Mohamed , Wang Zijin
Published: December 2021

Purpose

This study aims to evaluate the influence of connected and autonomous vehicle (CAV) merging algorithms on the driver behavior of human-driven vehicles on the mainline.

Design/methodology/approach

Previous studies designed their merging algorithms mostly based on either the simulation or the restricted field testing, which lacks consideration of realistic driving behaviors in the merging scenario. This study developed a multi-driver simulator system to embed realistic driving behavior in the validation of merging algorithms.

Findings

Four types of CAV merging algorithms were evaluated regarding their influences on driving safety and driving comfort of the mainline vehicle platoon. The results revealed significant variation of the algorithm influences. Specifically, the results show that the reference-trajectory-based merging algorithm may outperform the social-psychology-based merging algorithm which only considers the ramp vehicles.

Originality/value

To the best of the authors’ knowledge, this is the first time to evaluate a CAV control algorithm considering realistic driver interactions rather than by the simulation. To achieve the research purpose, a novel multi-driver driving simulator was developed, which enables multi-drivers to simultaneously interact with each other during a virtual driving test. The results are expected to have practical implications for further improvement of the CAV merging algorithm.
Design, simulation and optimisation of lattice structures for remote control aeroplane
Authors: Laporte Théo
Published: December 2021

Purpose

Thus, in this work the goal is to design, simulate and optimise a holder of a brushless motor in lattice structure to get the best performance in terms of mechanical strength, vibration absorption and lightness.

Design/methodology/approach

Nowadays, most manufacturers and designers' goal are to sell efficient products in mass to keep up or outrun competition. Medical, aeronautical, automobile and civil engineering sectors produce complex parts and products that encompasses multiple properties such as lightweight, energy absorbance, vibration reduction and stress resistant. Studies found that lattice structures are more and more useful in these fields since their characteristics satisfy complex behaviour.

Findings

The study's outcome suggests that the use of lattice structure reduces 60% of the actual motor holder mass while keeping the strength of the material, meeting initial specifications.

Research limitations/implications

The Ram capacity of the PC.

Practical implications

Light materials for aerospace engineering elongate the range of the unmanned aerial vehicle (UAV) to an extra range of flight.

Social implications

Situation awareness of the country border using surveillance drone and minimising the consumption of fuel.

Originality/value

The research allowed reducing 60% the actual holder mass.
Research on risk management of medical and health care integration projects based on fuzzy WINGS-G1
Authors: Zhang Yu , Xu Lan
Published: December 2021

Purpose

This study establishes a risk management system for medical and health care integration projects to address the problem of high-risk potential and a strong correlation between risk factors.

Design/methodology/approach

A new fuzzy WINGS-G1 model for identifying key risk factors in medical and health care integration projects is proposed by introducing the fuzzy theory and the concept of risk incidence into the Weighted Influence Non-linear Gauge System (WINGS) method.

Findings

The authors analyze the fluidity of project risk factors through complex networks to control direct risks and cut off risk transmission paths to provide a reference for risk control and prevention of medical and health care integration projects.

Originality/value

(1) The integration of fuzzy theory into the WINGS method solves the problem of strong subjectivity of expert scoring in the traditional WINGS method; (2) By the different probabilities of risk factors, the concept of risk incidence is introduced in the WINGS model, which is more conducive to the identification of the critical risk factors and the rational allocation and utilization of organizational resources; (3) The use of the complex network for risk interactivity analysis fully reflects the dynamic nature of risk factors in medical and health care integration projects.