bluuebunny/crossref_metadata_2025 huggingface dataset:
Cytoskeletal Remodeling in Cancer
Authors: Aseervatham Jaya
Published: November 2020

Successful metastasis depends on cell invasion, migration, host immune escape, extravasation, and angiogenesis. The process of cell invasion and migration relies on the dynamic changes taking place in the cytoskeletal components; actin, tubulin and intermediate filaments. This is possible due to the plasticity of the cytoskeleton and coordinated action of all the three, is crucial for the process of metastasis from the primary site. Changes in cellular architecture by internal clues will affect the cell functions leading to the formation of different protrusions like lamellipodia, filopodia, and invadopodia that help in cell migration eventually leading to metastasis, which is life threatening than the formation of neoplasms. Understanding the signaling mechanisms involved, will give a better insight of the changes during metastasis, which will eventually help targeting proteins for treatment resulting in reduced mortality and longer survival.
An Insight into the Algal Evolution and Genomics
Authors: Khan Amna Komal , Kausar Humera , Jaferi Syyada Samra , Drouet Samantha , Hano Christophe , Abbasi Bilal Haider , Anjum Sumaira
Published: November 2020

With the increase in biotechnological, environmental, and nutraceutical importance of algae, about 100 whole genomic sequences of algae have been published, and this figure is expected to double in the coming years. The phenotypic and ecological diversity among algae hints at the range of functional capabilities encoded by algal genomes. In order to explore the biodiversity of algae and fully exploit their commercial potential, understanding their evolutionary, structural, functional, and developmental aspects at genomic level is a pre-requisite. So forth, the algal genomic analysis revealed us that algae evolved through endosymbiotic gene transfer, giving rise to around eight phyla. Amongst the diverse algal species, the unicellular green algae Chlamydomonas reinhardtii has attained the status of model organism as it is an ideal organism to elucidate the biological processes critical to plants and animals, as well as commercialized to produce range of bio-products. For this review, an overview of evolutionary process of algae through endosymbiosis in the light of genomics, as well as the phylogenomic, studies supporting the evolutionary process of algae was reviewed. Algal genomics not only helped us to understand the evolutionary history of algae but also may have an impact on our future by helping to create algae-based products and future biotechnological approaches.
The Changes of qEEG Approximate Entropy during Test of Variables of Attention as a Predictor of Major Depressive Disorder
Authors: Chen Shao-Tsu , Ku Li-Chi , Chen Shaw-Ji , Shen Tsu-Wang
Published: November 2020

Evaluating brain function through biosignals remains challenging. Quantitative electroencephalography (qEEG) outcomes have emerged as a potential intermediate biomarker for diagnostic clarification in psychological disorders. The Test of Variables of Attention (TOVA) was combined with qEEG to evaluate biomarkers such as absolute power, relative power, cordance, and approximate entropy from covariance matrix images to predict major depressive disorder (MDD). EEG data from 18 healthy control and 18 MDD patients were monitored during the resting state and TOVA. TOVA was found to provide aspects for the evaluation of MDD beyond resting electroencephalography. The results showed that the prefrontal qEEG theta cordance of the control and MDD groups were significantly different. For comparison, the changes in qEEG approximate entropy (ApEn) patterns observed during TOVA provided features to distinguish between participants with or without MDD. Moreover, ApEn scores during TOVA were a strong predictor of MDD, and the ApEn scores correlated with the Beck Depression Inventory (BDI) scores. Between-group differences in ApEn were more significant for the testing state than for the resting state. Our results provide further understanding for MDD treatment selection and response prediction during TOVA.
The Positive Impact and Associated Mechanisms of Physical Activity on Mental Health in Underprivileged Children and Adolescents: An Integrative Review
Authors: Rose Lauren T. , Soundy Andrew
Published: November 2020

(1) Background: Review-based studies are required to consider the different designs and data that describe the association between physical activity and mental health for underprivileged children and adolescents. There is a particular need to identify mechanisms which could explain the association, and factors which influence the association in this population group. (2) Methods: An integrative review with a systematic search was conducted in three stages: (i) a systematic literature search on four databases from inception until May 2020. Studies were eligible if they examined underprivileged children, involved moderate to vigorous physical activity (MVPA) and had a focus on at least one outcome measure related to mental health. (ii) Two critical appraisal tools were used to assess quality across different study designs. (iii) Synthesis was undertaken in four stages, examining social, internal and physical outcomes and mechanisms. (3) Results: A total of 16 studies were included (2 qualitative; 12 quantitative and 2 mixed methods). Fifteen (15/16; 93.8%) showed an association between MVPA and a significant improvement in at least one mental health outcome. The most studied outcomes included: internal aspects (n = 14), significant effects were identified in 12/14 studies; social aspects (n = 6), significant effects were identified across all studies and finally physical aspects (n = 5), significant effects were found in 3/5 studies. Autonomy support was identified as a mechanism to explain the change, whilst age and gender influenced the strength of the association. (4) Conclusions: The findings provide confirmation of the link between MVPA and the improved mental health and wellbeing of disadvantaged children and adolescents. Future research must consider the long-term effect through longitudinal studies, along with determining whether any specific types of PA are more impactful than others.
Seismic Upgrading of RC Wide Beam–Column Joints Using Steel Jackets
Authors: Santarsiero Giuseppe , Masi Angelo
Published: November 2020

This study is devoted to experimentally investigate the seismic behaviour of reinforced concrete (RC) wide beam–column joints equipped with a steel jacketing seismic strengthening solution. To this end, three identical full-scale specimens have been tested under cyclic loading, one in the as-built condition and two after the application of the strengthening solutions. Details of selected solutions are described in the paper along with the experimental results which confirm how the application of simple and feasible steel interventions can effectively improve the seismic capacity of wide beam–column connections in RC frames, especially in terms of lateral load carrying capacity and energy dissipation.
Hydrodechlorination of CHClF2 (HCFC-22) over Pd–Pt Catalysts Supported on Thermally Modified Activated Carbon
Authors: Radlik Monika , Juszczyk Wojciech , Matus Krzysztof , Raróg-Pilecka Wioletta , Karpiński Zbigniew
Published: November 2020

Commercial activated carbon, pretreated in helium at 1600 °C and largely free of micropores, was used as a support for two series of 2 wt.% Pd–Pt catalysts, prepared by impregnating the support with metal acetylacetonates or metal chlorides. The catalysts were characterized by temperature-programmed methods, H2 chemisorption, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and scanning transmission electron microscopy (STEM) with energy dispersive spectroscopy (EDS). Overall, the results confirmed the existence of well-dispersed Pd–Pt nanoparticles in the bimetallic catalysts, ranging in size from 2 to 3 nm. The catalysts were investigated in the gas phase hydrodechlorination of chlorodifluoromethane (HCFC-22). In this environmentally relevant reaction, both the ex-chloride and ex-acetylacetonate Pd–Pt/C catalysts exhibited better hydrodechlorination activity than the monometallic catalysts, which is consistent with the previous results of hydrodechlorination for other chlorine-containing compounds. This synergistic effect can be attributed to the electron charge transfer from platinum to palladium. In general, product selectivity changes regularly with Pd–Pt alloy composition, from high in CH2F2 for Pd/C (70–80%) to the selective formation of CH4 for Pt/C (60–70%).
Bioconversion of Sweet Sorghum Residues by Trichoderma citrinoviride C1 Enzymes Cocktail for Effective Bioethanol Production
Authors: Kancelista Anna , Chmielewska Joanna , Korzeniowski Paweł , Łaba Wojciech
Published: November 2020

Improved cost-effective bioethanol production using inexpensive enzymes preparation was investigated. Three types of waste lignocellulosic materials were converted—for the production of enzyme preparation, a mixture of sugar beet pulp and wheat bran, while the source of sugars in hydrolysates was sweet sorghum biomass. A novel enzyme cocktail of Trichoderma citrinoviride C1 is presented. The one-step ultrafiltration process of crude enzyme extract resulted in a threefold increase of cellulolytic and xylanolytic activities. The effectiveness of enzyme preparation, compared to Cellic® CTec2, was tested in an optimized enzymatic hydrolysis process. Depending on the test conditions, hydrolysates with different glucose concentrations were obtained—from 6.3 g L−1 to 14.6 g L−1 (representing from 90% to 79% of the CTec2 enzyme yield, respectively). Furthermore, ethanol production by Saccharomyces cerevisiae SIHA Active Yeast 6 strain DF 639 in optimal conditions reached about 120 mL kg d.m.−1 (75% compared with the CTec2 process). The achieved yields suggested that the produced enzyme cocktail C1 could be potentially used to reduce the cost of bioethanol production from sweet sorghum biomass.
Combination of Photodynamic Therapy and a Flagellin-Adjuvanted Cancer Vaccine Potentiated the Anti-PD-1-Mediated Melanoma Suppression
Authors: Hwang Hye Suk , Cherukula Kondareddy , Bang Yong Jun , Vijayan Veena , Moon Myeong Ju , Thiruppathi Jayalakshmi , Puth Sao , Jeong Yong Yeon , Park In-Kyu , Lee Shee Eun , Rhee Joon Haeng
Published: November 2020

Immune checkpoint inhibitors become a standard therapy for malignant melanoma. As immune checkpoint inhibitor monotherapies proved to have limited efficacy in significant portion of patients, it is envisaged that combination with other therapeutic modalities may improve clinical outcomes. We investigated the effect of combining photodynamic therapy (PDT) and TLR5 agonist flagellin-adjuvanted tumor-specific peptide vaccination (FlaB-Vax) on the promotion of PD-1 blockade-mediated melanoma suppression using a mouse B16-F10 implantation model. Using a bilateral mouse melanoma cancer model, we evaluated the potentiation of PD-1 blockade by the combination of peritumoral FlaB-Vax delivery and PDT tumor ablation. A photosensitizing agent, pheophorbide A (PhA), was used for laser-triggered photodynamic destruction of the primary tumor. The effect of combination therapy in conjunction with PD-1 blockade was evaluated for tumor growth and survival. The effector cytokines that promote the activation of CD8+ T cells and antigen-presenting cells in tumor tissue and tumor-draining lymph nodes (TDLNs) were also assayed. PDT and FlaB-Vax combination therapy induced efficacious systemic antitumor immune responses for local and abscopal tumor control, with a significant increase in tumor-infiltrating effector memory CD8+ T cells and systemic IFNγ secretion. The combination of PDT and FlaB-Vax also enhanced the infiltration of tumor antigen-reactive CD8+ T cells and the accumulation of migratory CXCL10-secreting CD103+ dendritic cells (DCs) presumably contributing to tumor antigen cross-presentation in the tumor microenvironment (TME). The CD8+ T-cell-dependent therapeutic benefits of PDT combined with FlaB-Vax was significantly enhanced by a PD-1-targeting checkpoint inhibitor therapy. Conclusively, the combination of FlaB-Vax with PDT-mediated tumor ablation would serve a safe and feasible combinatorial therapy for enhancing PD-1 blockade treatment of malignant melanoma.
Forecasting Intense Cut-Off Lows in South Africa Using the 4.4 km Unified Model
Authors: Muofhe Tshimbiluni Percy , Chikoore Hector , Bopape Mary-Jane Morongwa , Nethengwe Nthaduleni Samuel , Ndarana Thando , Rambuwani Gift Tshifhiwa
Published: November 2020

Mid-tropospheric cut-off low (COL) pressure systems are linked to severe weather, heavy rainfall and extreme cold conditions over South Africa. They occur during all the above and often result in floods and snowfalls during the winter months, disrupting economic activities and causing extensive damage to infrastructure. This paper examines the evolution and circulation patterns associated with cases of severe COLs over South Africa. We evaluate the performance of the 4.4 km Unified Model (UM) which is currently used operationally by the South African Weather Service (SAWS) to simulate daily rainfall. Circulation variables and precipitation simulated by the UM were compared against European Centre for Medium-Range Weather Forecast’s (ECMWF’s) ERA Interim re-analyses and GPM precipitation at 24-hour timesteps. We present five recent severe COLs, which occurred between 2016 and 2019, that had high impact and found a higher model skill when simulating heavy precipitation during the initial stages than the dissipating stages of the systems. A key finding was that the UM simulated the precipitation differently during the different stages of development and location of the systems. This is mainly due to inaccurate placing of COL centers. Understanding the performance and limitations of the UM model in simulating COL characteristics can benefit severe weather forecasting and contribute to disaster risk reduction in South Africa.
Application of AFM-Based Techniques in Studies of Corrosion and Corrosion Inhibition of Metallic Alloys
Authors: Yasakau Kiryl
Published: November 2020

In this review several scanning probe microscopy techniques are briefly discussed as valuable assets for corrosionists to study corrosion susceptibility and inhibition of metals and alloys at sub-micrometer resolution. At the beginning, the review provides the reader with background of atomic force microscopy (AFM) and related techniques such as scanning Kelvin probe force microscopy (SKPFM) and electrochemical AFM (EC-AFM). Afterwards, the review presents the current state of corrosion research and specific applications of the techniques in studying important metallic materials for the aircraft and automotive industries. Different corrosion mechanisms of metallic materials are addressed emphasizing the role of intermetallic inclusions, grain boundaries, and impurities as focal points for corrosion initiation and development. The presented information demonstrates the importance of localized studies using AFM-based techniques in understanding corrosion mechanisms of metallic materials and developing efficient means of corrosion prevention.
Research on the Influence of Carbonation on the Content and State of Chloride Ions and the Following Corrosion Resistance of Steel Bars in Cement Paste
Authors: Wang Hui , Zhang Ailian , Zhang Linchun , Liu Junzhe , Han Yan , Wang Jianmin
Published: November 2020

In this paper, the changes of free chloride ion concentration and bound chloride concentration in cement paste with different total and proportion of mineral admixtures under carbonation were studied. Moreover, the following corrosion resistance of steel bars buried in cement paste under carbonation was researched by testing the electrical resistance and alternating current (AC) impedance spectroscopy of reinforced cement paste. Results indicated that fly ash and granulated blast furnace slag powder with the content less than 20% by mass ratio of total binder hindered the solidification of chloride ions in cement-based materials, and blast furnace slag powder with the content higher than 20% promoted the solidification of chloride ions in cement paste. The carbonation effect was able to decrease the amount of solidified chloride ion and increase the amount of free chloride ions leading to accelerating the corrosion of reinforcement. Meanwhile, the blast furnace slag powder with the content higher than 20% could effectively promote the corrosion resistance of steel bars.
A Performance Comparison between Automated Deep Learning and Dental Professionals in Classification of Dental Implant Systems from Dental Imaging: A Multi-Center Study
Authors: Lee Jae-Hong , Kim Young-Taek , Lee Jong-Bin , Jeong Seong-Nyum
Published: November 2020

In this study, the efficacy of the automated deep convolutional neural network (DCNN) was evaluated for the classification of dental implant systems (DISs) and the accuracy of the performance was compared against that of dental professionals using dental radiographic images collected from three dental hospitals. A total of 11,980 panoramic and periapical radiographic images with six different types of DISs were divided into training (n = 9584) and testing (n = 2396) datasets. To compare the accuracy of the trained automated DCNN with dental professionals (including six board-certified periodontists, eight periodontology residents, and 11 residents not specialized in periodontology), 180 images were randomly selected from the test dataset. The accuracy of the automated DCNN based on the AUC, Youden index, sensitivity, and specificity, were 0.954, 0.808, 0.955, and 0.853, respectively. The automated DCNN outperformed most of the participating dental professionals, including board-certified periodontists, periodontal residents, and residents not specialized in periodontology. The automated DCNN was highly effective in classifying similar shapes of different types of DISs based on dental radiographic images. Further studies are necessary to determine the efficacy and feasibility of applying an automated DCNN in clinical practice.
Ophthalmic Evaluation of Diagnosed Cases of Eye Cystinosis: A Tertiary Care Center’s Experience
Authors: Kowalczyk Malgorzata , Toro Mario Damiano , Rejdak Robert , Załuska Wojciech , Gagliano Caterina , Sikora Przemyslaw
Published: November 2020

Background: We aimed to identify diagnosed cases of ocular cystinosis and describe clinical, epidemiological and therapeutic characteristics. Methods: This is a descriptive and retrospective case series. All patients underwent a full check-up examination every 4–6 months by ophthalmologists, nephrologists and other required specialists. Results: Of the seven cases, six (85.7%) were females and one (14.2%) was male. The infantile nephropathic form of cystinosis was observed in five patients and the juvenile nephropathic form in two patients. No patients with the ocular form of cystinosis were identified. Corneal cystine crystals (CCC) were found in all analyzed patients. Severe ocular and general complications of the disease that had been standing for years, connected to the infantile nephropathic form, delayed diagnosis or inappropriate treatment, were observed only in two patients. All patients received topical therapy. No adverse events related to the therapy were observed. Conclusions: Cystinosis is a rare, progressive disease. Early diagnosis and treatment prevent serious complications from numerous systemic organs. Patients require constant systematic monitoring by various specialists.
A Blind Calibration Model for I/Q Imbalances of Wideband Zero-IF Receivers
Authors: Peng Xiaoye , Wang Zhiyu , Mo Jiongjiong , Wang Chenge , Liu Jiarui , Yu Faxin
Published: November 2020

Frequency-dependent I/Q imbalance and frequency-independent I/Q imbalance are the major impairments in wideband zero-IF receivers, and they both cannot be ignored. In this paper, a blind calibration model is designed for compensating these I/Q imbalances. In order to accurately estimate the imbalance parameters with low cost, a classification rule is proposed according to the frequency-domain statistical characteristics of the received signal. The calibration points in the frequency-domain are divided into two groups. Then, the amplitude imbalance and the frequency-dependent phase imbalance are derived from the group of signal points and, separately, the frequency-independent phase imbalance is calculated from the group of noise points. In the derivation of the frequency-dependent phase imbalance, a general fitting model suitable for all signal points is proposed, which does not require special calculations for either DC point or fs/2 point. Then, a finite impulse response (FIR) real-valued filter is designed to correct the impairments of received signal. The performances of the proposed calibration model are evaluated through both simulations and experiments. The simulation results show the image rejection ratio (IRR) improvement to around 35–45 dBc at high signal-to-noise ratio (SNR). Based on the mismatched data of the ADRV9009 evaluation board, the experimental results exhibit the IRR improvement of both multi-tone and wideband signals to about 30 dBc.
Effective Beamforming Technique Amid Optimal Value for Wireless Communication
Authors: Qaisar Zahid Hussain , Irfan Muhamamd , Ali Tariq , Ahmad Ashfaq , Ali Ghulam , Glowacz Adam , Glowacz Witold , Caesarendra Wahyu , Mashraqi Aisha Mousa , Draz Umar , Hussain Shafiq
Published: November 2020

In the notion of communication system resource provision specifically, beam-forming is a concept of proficient utilization of the power of transmission. Network densification and massive MIMO allows us to control the power efficiency and can be effectively distributed among different users by reducing cost. We presented a practical scenario for the performance of massive MIMO and multi-small cell system to analyze the overall performance of the system. Our work is based on the resource allocation with optimal structural constraints to maintain the cost effectiveness while considering economic implications. The base stations located far away from the users receive attenuated signals and give rise to path loss, whereas the problems of inter cell interference also arise due to transmission from a base station to others cells. The performance of the cellular system can be enhanced with the combination of massive Mimo and small cells, where we simulate and also provide an analysis on practical system with optimal and low complexity beam-forming. The proposed scenario illustrates a structure with an optimal linear transmit beamforming regarding an efficient number of parameters to not lose optimality, which is extendable to designate any specific cellular network in consideration. Our approach exploited schemes with low complexity that are facilitating in complete solution formation, and tested them in various and all possible cases and scenarios.
A RISC-V Processor Design for Transparent Tracing
Authors: Gamino del Río Iván , Martínez Hellín Agustín , R. Polo Óscar , Jiménez Arribas Miguel , Parra Pablo , da Silva Antonio , Sánchez Jonatan , Sánchez Sebastián
Published: November 2020

Code instrumentation enables the observability of an embedded software system during its execution. A usage example of code instrumentation is the estimation of “worst-case execution time” using hybrid analysis. This analysis combines static code analysis with measurements of the execution time on the deployment platform. Static analysis of source code determines where to insert the tracing instructions, so that later, the execution time can be captured using a logic analyser. The main drawback of this technique is the overhead introduced by the execution of trace instructions. This paper proposes a modification of the architecture of a RISC pipelined processor that eliminates the execution time overhead introduced by the code instrumentation. In this way, it allows the tracing to be non-intrusive, since the sequence and execution times of the program under analysis are not modified by the introduction of traces. As a use case of the proposed solution, a processor, based on RISC-V architecture, was implemented using VHDL language. The processor, synthesized on a FPGA, was used to execute and evaluate a set of examples of instrumented code generated by a “worst-case execution time” estimation tool. The results validate that the proposed architecture executes the instrumented code without overhead.
Thermal Performance of an Energy Pile Group with a Deeply Penetrating U-Shaped Heat Exchanger
Authors: Lyu Weidong , Pu Hefu , Chen Jiannan (Nick)
Published: November 2020

This study presents a novel heat exchanger configuration, called a deeply penetrating U-shaped configuration, for energy piles. The outlet water temperature, temperature variation along the tube, and heat transfer rate are simulated and computed using Comsol Multiphysics software. The simulations are for the cooling mode. The proposed configuration is compared with traditional U-shaped and W-shaped configurations to prove its superiority. The thermal performance of the pile group is compared with that of a single pile to investigate the effects of the pile group on the heat transfer. A parametric analysis is performed to investigate the effects of several important parameters (i.e., pile spacing, pile diameter, soil type, and thermal parameters) on the heat transfer performance of an energy pile group with the proposed deeply penetrating U-shaped configuration. The results indicate that the corner pile indicates a nonnegligible heat transfer rate 6.8% and 9.9% higher than the central pile in quincuncial and squared arrangements. Purely from the standpoint of thermal performance, the pile spacing is recommended to be more than 6.8 times the pile diameter to reduce the influence of the pile group on the heat transfer capacity.
Energy and Resource Efficiency in Apatite-Nepheline Ore Waste Processing Using the Digital Twin Approach
Authors: Dli Maksim , Puchkov Andrei , Meshalkin Valery , Abdeev Ildar , Saitov Rail , Abdeev Rinat
Published: November 2020

The paper presents a structure of the digital environment as an integral part of the “digital twin” technology, and stipulates the research to be carried out towards an energy and recourse efficiency technology assessment of phosphorus production from apatite-nepheline ore waste. The problem with their processing is acute in the regions of the Russian Arctic shelf, where a large number of mining and processing plants are concentrated; therefore, the study and creation of energy-efficient systems for ore waste disposal is an urgent scientific problem. The subject of the study is the infoware for monitoring phosphorus production. The applied study methods are based on systems theory and system analysis, technical cybernetics, machine learning technologies as well as numerical experiments. The usage of “digital twin” elements to increase the energy and resource efficiency of phosphorus production is determined by the desire to minimize the costs of production modernization by introducing advanced algorithms and computer architectures. The algorithmic part of the proposed tools for energy and resource efficiency optimization is based on the deep neural network apparatus and a previously developed mathematical description of the thermophysical, thermodynamic, chemical, and hydrodynamic processes occurring in the phosphorus production system. The ensemble application of deep neural networks allows for multichannel control over the phosphorus technology process and the implementation of continuous additional training for the networks during the technological system operation, creating a high-precision digital copy, which is used to determine control actions and optimize energy and resource consumption. Algorithmic and software elements are developed for the digital environment, and the results of simulation experiments are presented. The main contribution of the conducted research consists of the proposed structure for technological information processing to optimize the phosphorus production system according to the criteria of energy and resource efficiency, as well as the developed software that implements the optimization parameters of this system.
Biofortification of Chicken Eggs with Vitamin K—Nutritional and Quality Improvements
Authors: O’Sullivan Siobhan M. , E. Ball M. Elizabeth , McDonald Emma , Hull George L. J. , Danaher Martin , Cashman Kevin D.
Published: November 2020

National nutrition surveys have shown that over half of all adults in Ireland, the United Kingdom (UK), and the United States of America (USA) have low vitamin K intakes. Thus, dietary strategies to improve vitamin K intakes are needed, and vitamin K biofortification of food may be one food-based approach. The primary aim of our study was to establish whether increasing the vitamin K3 content of hen feed can increase the vitamin K content of eggs, and the secondary aims were to examine the effects on hen performance parameters, as well as egg and eggshell quality parameters. A 12 week hen feeding trial was conducted in which Hyline chickens were randomized into four treatment groups (n = 32/group) and fed diets containing vitamin K3 (as menadione nicotinamide bisulfite) at 3 (control), 12.9, 23.7, and 45.7 mg/kg feed. Vitamin K1, menaquinone (MK)-4, MK-7, and MK-9 were measured in raw whole eggs via a liquid chromatography tandem mass spectrometry method. MK-4 was the most abundant form of vitamin K (91–98%) found in all eggs. Increasing the vitamin K3 content of hen feed over the control level significantly (p < 0.001) enhanced the MK-4 content of eggs (mean range: 46–51 µg/100 g, representing ~42–56% of US Adequate Intake values). Vitamin K biofortification also led to significant (p < 0.05) increases in the yellowness of egg yolk and in eggshell weight and thickness, but no other changes in egg quality or hen performance parameters. In conclusion, high-quality vitamin K-biofortified eggs can be produced with at least double the total vitamin K content compared to that in commercially available eggs.
The Profiles of Low Molecular Nitrogen Compounds and Fatty Acids in Wort and Beer Obtained with the Addition of Quinoa (Chenopodium quinoa Willd.), Amaranth (Amaranthus cruentus L.) or Maltose Syrup
Authors: Bogdan Paulina , Kordialik-Bogacka Edyta , Czyżowska Agata , Oracz Joanna , Żyżelewicz Dorota
Published: November 2020

Replacement of a part of malt with unmalted materials is a common practice in beer production. These materials may differ in chemical composition than barley malt, which in turn can contribute to changes in the final composition of the wort. Consequently, it may affect yeast metabolism and final parameters of the obtained products. In this research, two unmalted pseudocereals were used: quinoa (Chenopodium quinoa Willd.) and amaranth (Amaranthus cruentus L.). Maltose syrup was tested as a reference material due to its commercial usage as a substitute of malt in production of worts. Replacement of a part of the malt with quinoa or amaranth favorably influenced the profiles of amino and fatty acids. Due to the fact that the type and concentration of individual amino acids and fatty acids in the fermented wort significantly affect the flavor compounds synthesized by yeast, differences in the profiles of esters and higher alcohol have been noted in beers produced with pseudocereals.
Characteristics and Antioxidant Potential of Cold-Pressed Oils—Possible Strategies to Improve Oil Stability
Authors: Grajzer Magdalena , Szmalcel Karolina , Kuźmiński Łukasz , Witkowski Mateusz , Kulma Anna , Prescha Anna
Published: November 2020

The relations of the antiradical capacity to oxidative stability parameters and the contents of fatty acids, sterols, tocopherols, phenols, flavonoids, chlorophyll, Cu, and Fe were assessed in 33 cold-pressed seed oils: Walnut (7 brands of oils), rosehip (3), camelina (6), milk thistle (5), flax (6), and pumpkin (6). The antiradical capacity of oils depended strongly on tocopherol contents with a synergistic effect with polyphenols. The efficacy of tocopherols in cold-pressed oils was accompanied by a negative correlation of their antioxidant capacity with the peroxide value increase after 3 months of shelf life. This study also showed a positive correlation between the content of phytosterols and the antiradical capacity in the lipophilic fraction of cold-pressed oils rich in n-3 polyunsaturated fatty acids (PUFAs). Multiple regression analysis identified groups of antioxidants naturally occurring in cold-pressed oils in relation to their fatty acid composition, which added to the cold-pressed oils could provide possible strategies to improve their stability. Achieving high stability is primarily a result of high phytosterol content exceeding the molar ratio of 1:100 for total phytosterols to α-linolenic acid. However, the molar ratios of tocopherols to linoleic acid below 1:2000 and polyphenols to linoleic acid below 1:3000 does not prevent oxidation in oils with the predominance of linoleic acid.
Monitoring and Evaluation of Sandstone Decay Adopting Non-Destructive Techniques: On-Site Application on Building Stones
Authors: Salvatici Teresa , Calandra Sara , Centauro Irene , Pecchioni Elena , Intrieri Emanuele , Garzonio Carlo Alberto
Published: November 2020

This paper focuses on the characterization approach to evaluate the decay state of Pietra Serena of historic buildings in Florence (Italy). Pietra Serena is a Florentine sandstone largely used in the city especially during the Renaissance; it is a symbol of cultural heritage of Florence and constitutes a large part of the city center, which was named a World Heritage Site by UNESCO in 1982. Unfortunately, many environmental factors negatively affect the stone, increasing damage and the danger of falling material. Any detachment of stone fragments, in addition to constitute a loss in cultural heritage, can be dangerous for citizens and the many tourists that visit the city. The use of non-destructive techniques (NDTs) as ultrasonic and Schmidt hammer tests can quantitatively define some mechanical properties and help to monitor the decay degree of building stone. In this study, the NDTs were combined with mineralogical, petrographical, chemical and physical analyses to investigate the stone materials, in order to correlate their features with the characteristics of the different artefacts in Pietra Serena. Correlations between the NDTs results and the compositional characteristics of the on-site stone were carried out; such discussion allows to identify zones of weakness and dangerous unstable elements.
The Relationship between Flourishing and Depression in Children in the U.S. Using a Socioecological Perspective
Authors: Jang Chang-Yong , Cho Eun-Hyung , Kwak Yi-Sub , Kim TaeEung
Published: November 2020

Children’s flourishing is likely to be associated with achieving a positive mental and physical quality of life, which is considered as an important factor for helping children to overcome psychological adversity during the critical stage of emotional development. This study examined the relationships between children’s flourishing and childhood depression. This was a cross-sectional study using the 2011–2012 National Children’s Health Survey in the U.S. The conceptual framework that guided this study was a modified ecological system theory model. Multiple regressions were performed to investigate the associations between flourishing and pediatric depression, controlling for demographics, physical activity-related behaviors, family and environmental conditions. A total of 45,309 children (representing 33,293,823 children at the population level) were identified in this study (mean age: 13.63 years; female: 48.7%). Children’s childhood depression was highly related to direct parenting functions, individual needs and environmental availabilities and accessibilities from a socioecological perspective. This study revealed multiple dimensions of how sociological factors influence children’s flourishing and mental health. Parents’ involvement in children’s physical activities and family and social support are crucial for children’s flourishing and mental health status. More attention needs to be paid to provide children with family and social support to help them to overcome and reduce childhood depression.
Identification, Trend Analysis and Influencing Factors of Mental Health Status of the Chinese Older Adults
Authors: Yang Hongyan , Ma Jun , Hu Hongwei , Li Fanjie
Published: November 2020

This study aimed to analyse the classification, development trends and the influencing factors of the Chinese older adults’ mental health state. Based on longitudinal data of Chinese older adults from 2005 to 2014, 2077 older adults aged 64 to 105 were included and the Latent Class Model, Latent Growth Mixture Model and Multinomial Logit models were employed in this study. We find that there are three types of mental health state of the Chinese older adults: negative, positive and contradictory; and the contradictory type could easily turn into negative or positive mental health state. There are four types of dynamic trends of mental health state: persistently negative, persistently positive, pro-negative, and pro-positive. About 40% of the older adults could maintain positive mental health state, and the pro-negative accounts for larger proportion than the pro-positive. Better economic status, good living habits, cohabitation with family members and pension coverage are beneficial for positive mental health state of the Chinese older adults. There is significant heterogeneity in the state as well as development trends of mental health of the older adults. The older adults with contradictory and negative types of mental health state should get timely psychological help to avoid turning into negative state. A series of polices are needed to promote mental health for the older adults in China.
Thermodynamics and Kinetics of Glycolytic Reactions. Part I: Kinetic Modeling Based on Irreversible Thermodynamics and Validation by Calorimetry
Authors: Vogel Kristina , Greinert Thorsten , Reichard Monique , Held Christoph , Harms Hauke , Maskow Thomas
Published: November 2020

In systems biology, material balances, kinetic models, and thermodynamic boundary conditions are increasingly used for metabolic network analysis. It is remarkable that the reversibility of enzyme-catalyzed reactions and the influence of cytosolic conditions are often neglected in kinetic models. In fact, enzyme-catalyzed reactions in numerous metabolic pathways such as in glycolysis are often reversible, i.e., they only proceed until an equilibrium state is reached and not until the substrate is completely consumed. Here, we propose the use of irreversible thermodynamics to describe the kinetic approximation to the equilibrium state in a consistent way with very few adjustable parameters. Using a flux-force approach allowed describing the influence of cytosolic conditions on the kinetics by only one single parameter. The approach was applied to reaction steps 2 and 9 of glycolysis (i.e., the phosphoglucose isomerase reaction from glucose 6-phosphate to fructose 6-phosphate and the enolase-catalyzed reaction from 2-phosphoglycerate to phosphoenolpyruvate and water). The temperature dependence of the kinetic parameter fulfills the Arrhenius relation and the derived activation energies are plausible. All the data obtained in this work were measured efficiently and accurately by means of isothermal titration calorimetry (ITC). The combination of calorimetric monitoring with simple flux-force relations has the potential for adequate consideration of cytosolic conditions in a simple manner.
Prevention of Melanoma Extravasation as a New Treatment Option Exemplified by p38/MK2 Inhibition
Authors: Petzelbauer Peter
Published: November 2020

Melanoma releases numerous tumor cells into the circulation; however, only a very small fraction of these cells is able to establish distant metastasis. Intravascular survival of circulating tumor cells is limited through hemodynamic forces and by the lack of matrix interactions. The extravasation step is, thus, of unique importance to establish metastasis. Similar to leukocyte extravasation, this process is under the control of adhesion molecule pairs expressed on melanoma and endothelial cells, and as for leukocytes, ligands need to be adequately presented on cell surfaces. Based on melanoma plasticity, there is considerable heterogeneity even within one tumor and one patient resulting in a mixture of invasive or proliferative cells. The molecular control for this switch is still ill-defined. Recently, the balance between two kinase pathways, p38 and JNK, has been shown to determine growth characteristics of melanoma. While an active JNK pathway induces a proliferative phenotype with reduced invasive features, an active p38/MK2 pathway results in an invasive phenotype and supports the extravasation step via the expression of molecules capable of binding to endothelial integrins. Therapeutic targeting of MK2 to prevent extravasation might reduce metastatic spread.
The Influence of Tumor Microenvironment on Immune Escape of Melanoma
Authors: Simiczyjew Aleksandra , Dratkiewicz Ewelina , Mazurkiewicz Justyna , Ziętek Marcin , Matkowski Rafał , Nowak Dorota
Published: November 2020

The low efficiency of currently-used anti-cancer therapies poses a serious challenge, especially in the case of malignant melanoma, a cancer characterized by elevated invasiveness and relatively high mortality rate. The role of the tumor microenvironment in the progression of melanoma and its acquisition of resistance to treatment seems to be the main focus of recent studies. One of the factors that, in normal conditions, aids the organism in its fight against the cancer and, following the malignant transformation, adapts to facilitate the development of the tumor is the immune system. A variety of cell types, i.e., T and B lymphocytes, macrophages, and dendritic and natural killer cells, as well as neutrophils, support the growth and invasiveness of melanoma cells, utilizing a plethora of mechanisms, including secretion of pro-inflammatory molecules, induction of inhibitory receptors expression, or depletion of essential nutrients. This review provides a comprehensive summary of the processes regulated by tumor-associated cells that promote the immune escape of melanoma cells. The described mechanisms offer potential new targets for anti-cancer treatment and should be further studied to improve currently-employed therapies.
Cadmium Stress Reprograms ROS/RNS Homeostasis in Phytophthora infestans (Mont.) de Bary
Authors: Gajewska Joanna , Azzahra Nur Afifah , Bingöl Özgün Ali , Izbiańska-Jankowska Karolina , Jelonek Tomasz , Deckert Joanna , Floryszak-Wieczorek Jolanta , Arasimowicz-Jelonek Magdalena
Published: November 2020

Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato, growing under moderate cadmium stress (Cd, 5 mg/L) showed nitro-oxidative imbalance associated with an enhanced antioxidant response. Cadmium notably elevated the level of nitric oxide, superoxide and peroxynitrite that stimulated nitrative modifications within the RNA and DNA pools in the phytopathogen structures. In contrast, the protein pool undergoing nitration was diminished confirming that protein tyrosine nitration is a flexible element of the oomycete adaptive strategy to heavy metal stress. Finally, to verify whether Cd is able to modify P. infestans pathogenicity, a disease index and molecular assessment of disease progress were analysed indicating that Cd stress enhanced aggressiveness of vr P. infestans towards various potato cultivars. Taken together, Cd not only affected hyphal growth rate and caused biochemical changes in P. infestans structures, but accelerated the pathogenicity as well. The nitro-oxidative homeostasis imbalance underlies the phytopathogen adaptive strategy and survival in the heavy metal contaminated environment.
Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes
Authors: Supruniuk Elżbieta , Mikłosz Agnieszka , Chabowski Adrian
Published: November 2020

Pyrroloquinoline quinone (PQQ) is a novel stimulator of mitochondrial biogenesis and cellular energy metabolism. This is the first study investigating regulatory mechanisms and metabolic responses underlying PQQ’s action in palmitate-exposed L6 myotubes. Particularly, we assessed alterations in lipid content and composition, expression of metabolic enzymes, and changes in glucose transport. The experiments were conducted using muscle cells subjected to short (2 h) and prolonged (24 h) incubation with PQQ in a sequence of pre- and post-palmitic acid (PA) exposure. We demonstrated the opposite effects of 2 and 24 h treatments with PQQ on lipid content, i.e., a decline in the level of free fatty acids and triacylglycerols in response to short-time PQQ incubation as compared to increases in diacylglycerol and triacylglycerol levels observed after 24 h. We did not demonstrate a significant impact of PQQ on fatty acid transport. The analysis of metabolic enzyme expression showed that the vast majority of PQQ-dependent alterations cumulated in the PA/PQQ 24 h group, including elevated protein amount of peroxisome proliferator activated receptor γ co-activator 1α (PGC-1α), sirtuin-1 (SIRT1), phosphorylated 5′AMP-activated protein kinase (pAMPK), carnitine palmitoyltransferase I (CPT1), citrate synthase (CS), fatty acid synthase (FAS), and serine palmitoyltransferase, long chain base subunit 1 (SPT1). In conclusion, the results mentioned above indicate PQQ-dependent activation of both fatty acid oxidation and lipid synthesis in order to adapt cells to palmitic acid-rich medium, although PQQ did not attenuate insulin resistance in muscle cells.
Changes of Body Weight and Body Composition in Obese Patients with Prader–Willi Syndrome at 3 and 6 Years of Follow-Up: A Retrospective Cohort Study
Authors: Bedogni Giorgio , Grugni Graziano , Cicolini Sabrina , Caroli Diana , Tamini Sofia , Sartorio Alessandro
Published: November 2020

Few short-term studies of weight loss have been performed in adult patients with Prader–Willi syndrome (PWS) undergoing metabolic rehabilitation. We performed a retrospective cohort study of 45 adult obese PWS patients undergoing a long-term multidisciplinary metabolic rehabilitation program based on diet and physical activity. Body composition was evaluated by dual-energy X-ray absorptiometry in 36 (80%) patients. The mean (95% CI) weight change was −3.6 (−7.6 to 0.4, p = 0.08) kg at 3 years and −4.6 (−8.5 to −0.8, p = 0.02) kg at 6 years, and that of BMI was −1.7 (−3.4 to 0.1, p = 0.06) kg/m2 at 3 years and −2.1 (−3.8 to −0.4, p = 0.02) kg/m2 at 6 years. A decrease of about 2% in fat mass per unit of body mass was observed, which is in line with the expectations for moderate weight loss. A possibly clinically relevant decrease in total and low-density lipoprotein cholesterol was also observed. These long-term results are important for patients with PWS, which is characterized by severe hyperphagia, behavioral disturbances, and cognitive impairment and is generally considered “resistant” to classical weight loss interventions.