bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectiveness of a Virtual-Reality-Based Self-Help Intervention for Lowering the Psychological Burden during the COVID-19 Pandemic: Results from a Randomized Controlled Trial in Iran
Authors: Farahimanesh Sharareh , Serino Silvia , Tuena Cosimo , Di Lernia Daniele , Wiederhold Brenda K. , Bernardelli Luca , Riva Giuseppe , Moradi Alireza
Published: March 2023

Background: The COVID-19 pandemic. In this framework, digital self-help interventions have the potential to provide flexible and scalable solutions for delivering evidence-based treatments that do not necessitate face-to-face meetings. Objective: as part of a multicentric project, the purpose of the current randomized controlled trial was to evaluate the efficacy of a Virtual-Reality-based self-help intervention (namely, COVID Feel Good) in lowering the psychological distress experienced during the COVID-19 pandemic in Iran. Methods: 60 participants were randomly assigned to the experimental (COVID Feel Good intervention group) or the control (no-treatment control group) condition. At the beginning of the intervention (Day 0), at the end of the intervention (Day 7), and after a 2-week follow-up (Day 21), measurements of depressive and anxiety levels, general distress, perceived levels of stress, hopelessness (primary outcome measures), perceived interpersonal closeness with the social world, and fear of COVID-19 (secondary outcome measure) were collected. The protocol consists of two integrated parts: the first part includes a relaxing 10-min three-hundred-sixty-degree (360°) video, while the second one includes social tasks with specified objectives. Results: In terms of the primary outcomes, participants in the COVID Feel Good intervention group improved in depression, stress, anxiety, and perceived stress but not hopelessness. Secondary outcome results showed an improvement in perceived social connectedness and a substantial decrease in fear of COVID-19. Conclusions: these findings on the efficacy of COVID Feel Good training add to the growing body of evidence demonstrating the feasibility of digital self-help interventions in promoting well-being during this unique period.
Comparison of the Relationship between Visual Acuity and Motor Function in Non-Elderly and Elderly Adults
Authors: Ito Sadayuki , Nakashima Hiroaki , Machino Masaaki , Segi Naoki , Ishizuka Shinya , Takegami Yasuhiko , Takeuchi Jun , Ouchida Jun , Hasegawa Yukiharu , Imagama Shiro
Published: March 2023

This study aimed to clarify the relationship between visual acuity and motor function in younger and elderly participants and to compare differences between non-elderly and elderly participants. In total, 295 participants who underwent visual and motor functional examinations were included; participants with visual acuity ≥0.7 were assigned to the normal group (N group) and those with visual acuity ≤0.7 were assigned to the low-visual-acuity group (L group). Motor function was compared between the N and L groups; the analysis was performed by grouping participants into those aged >65 years (elderly) and those aged <65 years (non-elderly). The non-elderly group (average age, 55.6 ± 6.7 years) had 105 and 35 participants in the N and L groups, respectively. Back muscle strength was significantly lower in the L group than in the N group. The elderly group (average age, 71.1 ± 5.1 years) had 102 and 53 participants in the N and L groups, respectively. Gait speed was significantly lower in the L group than in the N group. These results reveal differences in the relationship between vision and motor function in non-elderly and elderly adults and indicate that poor vision is associated with lower back-muscle strength and walking speed in younger and elderly participants, respectively.
Intraoperative Hypotension Is Associated with Postoperative Nausea and Vomiting in the PACU: A Retrospective Database Analysis
Authors: Maleczek Mathias , Laxar Daniel , Geroldinger Angelika , Kimberger Oliver
Published: March 2023

Multiple risk factors for postoperative nausea and vomiting (PONV)—a very distressing and outcome-related complication—have been identified, including female sex, absence of a history of smoking, history of PONV, and postoperative opioid use. Evidence of association of intraoperative hypotension with PONV is contradictory. A retrospective analysis of the perioperative documentation of 38,577 surgeries was conducted. The associations between different characterizations of intraoperative hypotension and PONV in the postoperative care unit (PACU) were investigated. First, the relationship between different characterizations of intraoperative hypotension with regard to PONV in the PACU was investigated. Secondly, the performance of the optimal characterization was assessed in an independent dataset derived via random split. The vast majority of characterizations showed an association of hypotension with the incidence of PONV in the PACU. In a multivariable regression, time with a MAP under 50 mmHg showed the strongest association with PONV in terms of the cross-validated Brier score. The adjusted odds for PONV in the PACU were estimated to be 1.34 times higher (95% CI: 1.33–1.35) when a MAP was under 50 mmHg for at least 1.8 min than when a MAP remained above 50 mmHg. The finding indicates that intraoperative hypotension may yet be another risk factor for PONV and therefore emphasizes the importance of intraoperative blood pressure management not only in patients at risk for cardiovascular complications but also in young and healthy patients at risk of PONV.
Difficulties in Defining Oligometastatic Prostate Cancer: Implications for Clinical Trial Accrual and Community Practice Adoption of Metastasis-Directed Therapy Approaches
Authors: Dorff Tanya Barauskas , Kasparian Saro , Garg Natasha , Liu Sandy , Pal Sumanta Kumar , Wong Jeffrey , Dandapani Savita
Published: March 2023

Background: Metastasis-directed therapy is widely utilized for oligometastatic prostate cancer patients, but standard imaging does not always identify metastases definitively and, even with PSMA PET, there may be equivocal findings. Not all clinicians have access to detailed imaging review, particularly outside of academic cancer centers, and PET scan access is also limited. We sought to understand how imaging interpretation impacted recruitment to a clinical trial for oligometastatic prostate cancer. Methods: IRB approval was obtained to review medical records from all patients screened for the institutional IRB-approved clinical trial for men with oligometastatic prostate cancer involving androgen deprivation plus stereotactic radiation to all metastatic sites, as well as radium223 (NCT03361735). Clinical trial inclusion required at least one bone metastatic lesion and no more than five total sites of metastasis, including soft tissue sites. Tumor board discussion records were reviewed, along with results from additional radiology studies ordered or confirmatory biopsies performed. Clinical characteristics such as PSA level and Gleason score were studied for association with likelihood of oligometastatic disease confirmation. Results: At the time of data analysis, 18 subjects were deemed eligible and 20 were not eligible. The most common reasons for ineligibility were no confirmed bone metastasis in 16 patients (59%) and too many metastatic sites in 3 (11%). The median PSA of eligible subjects was 3.28 (range 0.4–45.5), whereas the median PSA of those found to be ineligible was 10.45 (range 3.7–26.3) when there were too many metastases identified, and 2.7 (range 0.2–34.5) when metastases were unconfirmed. PET imaging (PSMA or fluciclovine PET) increased the number of metastases, while MRI resulted in downstaging to non-metastatic disease. Conclusions: This research suggests that additional imaging (i.e., at least two independent imaging modalities of a possible metastatic lesion) or tumor board adjudication of imaging findings may be critical to correctly identify patients appropriate for enrollment in oligometastatic protocols. This should be considered as trials of metastasis-directed therapy for oligometastatic prostate cancer accrue and results are translated to broader oncology practice.
Walking with Different Insoles Changes Lower-Limb Biomechanics Globally in Patients with Medial Knee Osteoarthritis
Authors: Jaques Guillaume , Ulrich Baptiste , Hoffmann Laurent , Jolles Brigitte M. , Favre Julien
Published: March 2023

Using insoles to modify walking biomechanics is of keen interest for the treatment of medial-compartment knee osteoarthritis. So far, insole interventions have focused on reducing the peak of the knee adduction moment (pKAM) and have led to inconsistent clinical outcomes. This study aimed to evaluate the changes in other gait variables related to knee osteoarthritis when patients walk with different insoles to provide insights into the necessity to enlarge the biomechanical analyses to other variables. Walking trials were recorded for 10 patients in four insole conditions. Changes among conditions were computed for six gait variables, including the pKAM. The associations between the changes in pKAM and the changes in the other variables were also assessed individually. Walking with different insoles had noticeable effects on the six gait variables, with high heterogeneity among patients. For all variables, at least 36.67% of the changes were of medium-to-large effect size. The associations with the changes in pKAM varied among variables and patients. In conclusion, this study showed that varying the insole could globally influence ambulatory biomechanics and that limiting measurement to the pKAM could lead to an important loss of information. Beyond the consideration of additional gait variables, this study also encourages personalized interventions to address inter-patient variability.
Local Recurrence after Endoscopic Submucosal Dissection of Early Gastric Cancer
Authors: Ryu Dae-Gon , Kim Su-Jin , Choi Cheol-Woong , Park Su-Bum , Nam Hyeong-Seok , Lee Si-Hak , Hwang Sun-Hwi
Published: March 2023

Endoscopic submucosal dissection (ESD) is considered the treatment of choice for early gastric cancer (EGC) with a negligible risk of lymph node metastasis. Locally recurrent lesions on artificial ulcer scars are difficult to manage. Predicting the risk of local recurrence after ESD is important to manage and prevent the event. We aimed to elucidate the risk factors associated with local recurrence after ESD of EGC. Between November 2008 and February 2016, consecutive patients (n = 641; mean age, 69.3 ± 9.5 years; men, 77.2%) with EGC who underwent ESD at a single tertiary referral hospital were retrospectively analyzed to evaluate the incidence and factors associated with local recurrence. Local recurrence was defined as the development of neoplastic lesions at or adjacent to the site of the post-ESD scar. En bloc and complete resection rates were 97.8% and 93.6%, respectively. The local recurrence rate after ESD was 3.1%. The mean follow-up period after ESD was 50.7 ± 32.5 months. One case of gastric cancer-related death (0.15%) was noted, wherein the patient had refused additive surgical resection after ESD for EGC with lymphatic and deep submucosal invasion. Lesion size ≥15 mm, incomplete histologic resection, undifferentiated adenocarcinoma, scar, and the absence of erythema of the surface were associated with a higher risk of local recurrence. Predicting local recurrence during regular endoscopic surveillance after ESD is important, especially in patients with a larger lesion size (≥15 mm), incomplete histologic resection, surface changes of scars, and no erythema of the surface.
Marine Natural Gas Hydrate Self-Entry Exploitation Device: Evaluation of Production Enhancement Measures
Authors: Wang Jianhua , Ye Hongyu , Chen Jingyu , Huang Qichao , Guo Gaoqiang , Huang Xuhong , Zi Mucong , Li Dayong , Wu Xuezhen
Published: March 2023

Test exploitation equipment and technology have progressed considerably in marine natural gas hydrate (NGH) exploitation, but many critical technical issues still need to be resolved before commercial production. Previous studies have proposed a non-drilling exploitation device—a self-entry exploitation device (SEED)—but reaching the NGH commercial exploitation threshold in its initial state is difficult. Consequently, we verified and evaluated some production enhancement measures to improve the exploitation system of the SEED. In this study, based on the geological data from the SHSC-4 site in the Shenhu sea and the material characteristics of the SEED, we carried out four production enhancement measures by numerical simulation. The results indicate that: (i) open-hole position adjustment can expand the contact areas between the device and NGH reservoirs; (ii) the effect of inner wall heating is limited but sufficient to achieve the goal of preventing clogging; (iii) it is necessary to select a reasonable spacing according to a combination of expected production cycle time and pressure when carrying out clustered depressurization; and (vi) when performing depressurization combined with thermal stimulation exploitation, factors such as permeability and thermal conductivity play a decisive factor in gas production.
Tuning the Model Winds in Perspective of Operational Storm Surge Prediction in the Adriatic Sea
Authors: De Biasio Francesco , Zecchetto Stefano
Published: March 2023

In the Adriatic Sea, the sea surface wind forecasts are often underestimated, with detrimental effects on the accuracy of sea level and storm surge predictions. Among the various causes, this mainly depends on the meteorological forcing of the wind. In this paper, we try to improve an existing numerical method, called “wind bias mitigation”, which relies on scatterometer wind observations to determine a multiplicative factor Δw, whose application to the model wind reduces its inaccuracy with respect to the scatterometer wind. Following four different mathematical approaches, we formulate and discuss seven new expressions of the multiplicative factor. The eight different expressions of the bias mitigation factor, the original one and the seven formulated in this study, are assessed with the aid of four datasets of real sea surface wind events in a variety of sea level conditions in the northern Adriatic Sea, several of which gave rise to high water events in the Venice Lagoon. The statistical analysis shows that some of the seven new formulations of the wind bias mitigation factor are able to lower the model-scatterometer bias with respect to the original formulation. For some other of the seven new formulations, the absolute bias, with respect to scatterometer, of the mitigated model wind field, results lower than that supplied by the unmodified model wind field in 81% of the considered storm surge events in the area of interest, against the 73% of the original formulation of the wind bias mitigation. This represents an 11% improvement in the bias mitigation process, with respect to the original formulation. The best performing of the seven new wind bias mitigation factors, that based on the linear least square regression of the squared wind speed (LLSRE), has been implemented in the operational sea level forecast chain of the Tide Forecast and Early Warning Centre of the Venice Municipality (CPSM), to provide support to the operation of the MO.SE. barriers in Venice.
Molluscs from Tidal Channels of the Gulf of Gabès (Tunisia): Quantitative Data and Comparison with Other Lagoons and Coastal Waters of the Mediterranean Sea
Authors: Fersi Abir , Pezy Jean-Philippe , Bakalem Ali , Neifar Lassad , Dauvin Jean-Claude
Published: March 2023

The present study analyses the spatio-temporal structuration of the molluscan fauna from four tidal channels of the Gulf of Gabès. A total of 26 stations were sampled at four seasons from March 2016 to January 2017, leading to the identification of 2695 individuals and 57 species. The species richness and abundances are higher in autumn than in other seasons. The fauna is dominated by seven species, three gastropods [Cerithium scabridum Philippi, 1848, Bittium reticulatum (da Costa, 1778) and Tricolia speciosa (Megerle von Mühfleld, 1824)] and four bivalves [Abra alba (W. Wood, 1802), Loripes orbiculatus Poli, 1791, Varicorbula gibba (Olivi, 1792) and Peronaea planata (Linnaeus, 1758)], which are characteristic of habitats with detritus accumulation and seagrass meadows. These dominant species are commonly recorded in lagoons and coastal shallow waters of the Mediterranean Sea. The structure of the molluscan fauna is linked to the location of tidal channels in the Gulf of Gabès. Abundances are lower in the Mimoun channel than in the other channels, especially the Maltine channel which shows a great accumulation of organic matter and high abundances of molluscs. Low abundances are found in high-energy hydrodynamic zones with gravel sediment; conversely, the presence of macrophytes (mainly in seagrass meadows) increases molluscan diversity. Comparisons with other sites in the shallow waters of the Tunisian coast and lagoons show that the taxonomic diversity of molluscs of the tidal channels of the Gulf of Gabès is equivalent to that reported elsewhere, but the abundance per m2 is among the lowest levels recorded here. Moreover, most of the dominant species found in the Gulf of Gabès tidal channel are reported as dominant in other studies covering the Mediterranean Sea. A distance-based redundancy analysis shows that depth, sediment type and the presence of marine phanerogams and filter-feeder bivalves on fine sands and gravels account for the structure of mollusc assemblages associated with each channel.
Post-Traumatic Single-Digit Onychomycosis
Authors: Haneke Eckart , Stovbyr Ganna
Published: March 2023

Onychomycoses are a group of fungal nail infections commonly classified either according to the pathogenic fungus, to the duration of the disease or to the mode of fungal invasion. Most cases are diagnosed clinically, although there is a general consensus that the pathogen should be identified prior to initiating a treatment. However, this is often difficult as the classical mycologic methods of direct microscopy and culture frequently remain negative. We came across a particular subset of onychomycoses, which posed extreme diagnostic and therapeutic challenges. Over a period of 15 years, 44 patients were seen in specialized nail clinics with a single nail dystrophy that was examined and treated in vain by many practitioners and dermatologists prior to their consultation. Of the forty-four cases, thirty-nine patients had a fingernail affected and five had a toenail affected. The nail was almost completely onycholytic, the nail bed visibly keratotic, the proximal nail fold smooth and shiny and slightly swollen. All patients except five brought the results of negative mycologic cultures. Thirty-four patients had received antifungal therapy, mostly topical, as a single nail would not qualify for systemic treatment according to most national and international guidelines. The diagnosis was finally confirmed by histopathology of the nail plate showing an invasive onychomycosis in all cases. After nail avulsion and combined topical and systemic antifungal therapy, thirty-six patients were cured, three were lost from follow-up, and five showed improved nails but not a complete clinical and mycologic cure. A single-digit nail disease raises the suspicion of a tumor or a trauma; although, in rare cases, diseases normally affecting several nails may only affect a single nail. Such a case should prompt the clinician to ask for a previous trauma to this digit and to intensify the search for a specific pathogen. This study also underlines the importance of histopathology for the diagnosis of onychomycoses.
The Fungal Metabolite (+)-Terrein Abrogates Inflammatory Bone Resorption via the Suppression of TNF-α Production in a Ligature-Induced Periodontitis Mouse Model
Authors: Sako Hidefumi , Omori Kazuhiro , Nakayama Masaaki , Mandai Hiroki , Ideguchi Hidetaka , Yoshimura-Nakagawa Saki , Sakaida Kyosuke , Nagata-Kamei Chiaki , Kobayashi Hiroya , Ishii Satoki , Ono Mitsuaki , Ibaragi Soichiro , Yamamoto Tadashi , Suga Seiji , Takashiba Shogo
Published: March 2023

Current periodontal treatment focuses on the mechanical removal of the source of infection, such as bacteria and their products, and there is no approach to control the host inflammatory response that leads to tissue destruction. In order to control periodontal inflammation, we have previously reported the optimization of (+)-terrein synthesis methods and the inhibitory effect of (+)-terrein on osteoclast differentiation in vitro. However, the pharmacological effect of (+)-terrein in vivo in the periodontitis model is still unknown. In this study, we investigated the effect of synthetic (+)-terrein on inflammatory bone resorption using a ligature-induced periodontitis mouse model. Synthetic (+)-terrein (30 mg/kg) was administered intraperitoneally twice a week to the mouse periodontitis model. The control group was treated with phosphate buffer. One to two weeks after the induction of periodontitis, the periodontal tissues were harvested for radiological evaluation (micro-CT), histological evaluation (HE staining and TRAP staining), and the evaluation of inflammatory cytokine production in the periodontal tissues and serum (quantitative reverse-transcription PCR, ELISA). The synthetic (+)-terrein-treated group suppressed alveolar bone resorption and the number of osteoclasts in the periodontal tissues compared to the control group (p < 0.05). In addition, synthetic (+)-terrein significantly suppressed both mRNA expression of TNF-α in the periodontal tissues and the serum concentration of TNF-α (both p < 0.05). In conclusion, we have demonstrated that synthetic (+)-terrein abrogates alveolar bone resorption via the suppression of TNF-α production and osteoclast differentiation in vivo. Therefore, we could expect potential clinical effects when using (+)-terrein on inflammatory bone resorption, including periodontitis.
Arthrodesis of the Proximal Interphalangeal Joint of the Finger—A Biomechanical Study of Primary Stability
Authors: Millrose Michael , Vonderlind Hans Christoph , Thannheimer Andreas , Ittermann Till , Rüther Johannes , Willauschus Maximilian , Bail Hermann-Josef , Eisenschenk Andreas
Published: March 2023

Background: Osteoarthritis of the proximal interphalangeal (PIP) joint of the finger often leads to global hand-function detriment. Different techniques for the arthrodesis of the proximal interphalangeal joint have been described that all lead to union in a reasonable percentage of patients and period of time. This biomechanical study aims to analyze and compare the primary stability of different techniques of arthrodesis to render postoperative immobilization unnecessary. Methods: Arthrodeses of 40° of composite cylinders were tested with different techniques in four-point bending for stability in extension as well as flexion. Results: In extension, the compression screw and the compression wires showed the highest stability—whereas in flexion, plate fusion was superior. Tension band, cerclage or compression screw fusion showed the best compromise in flexion/extension stability. Conclusions: Fusion techniques that apply compression to the fusion show superior stability. Cerclage, tension band and compression screws might be able to provide enough stability to withstand the forces exerted during unencumbered activities of daily living. Arthrodesis with plates should be limited to patients with special indications and require immobilization during consolidation.
Correction: Groen et al. Use of Image-Guided Surgical Navigation during Resection of Locally Recurrent Rectal Cancer. Life 2022, 12, 645
Authors: Groen Harald C. , den Hartog Anne G. , Heerink Wouter J. , Kuhlmann Koert F. D. , Kok Niels F. M. , van Veen Ruben , Hiep Marijn A. J. , Snaebjornsson Petur , Grotenhuis Brechtje A. , Beets Geerard L. , Aalbers Arend G. J. , Ruers Theo J. M.
Published: March 2023

The authors wish to make the following corrections to this paper [...]
Identification of Specific Biomarkers and Pathways in the Treatment Response of Infliximab for Inflammatory Bowel Disease: In-Silico Analysis
Authors: Kaddoura Rachid , Ghelani Hardik , Alqutami Fatma , Altaher Hala , Hachim Mahmood , Jan Reem Kais
Published: March 2023

Background: Inflammatory bowel disease (IBD) is characterized by chronic inflammation of the gastrointestinal tract. In biological therapy, infliximab became the first anti-tumor necrosis factor (TNF) agent approved for IBD. Despite this success, infliximab is expensive, often ineffective, and associated with adverse events. Prediction of infliximab resistance would improve overall potential outcomes. Therefore, there is a pressing need to widen the scope of investigating the role of genetics in IBD to their association with therapy response. Methods: In the current study, an in-silico analysis of publicly available IBD patient transcriptomics datasets from Gene Expression Omnibus (GEO) are used to identify subsets of differentially expressed genes (DEGs) involved in the pathogenesis of IBD and may serve as potential biomarkers for Infliximab response. Five datasets were found that met the inclusion criteria. The DEGs for datasets were identified using limma R packages through the GEOR2 tool. The probes’ annotated genes in each dataset intersected with DGEs from all other datasets. Enriched gene Ontology Clustering for the identified genes was performed using Metascape to explore the possible connections or interactions between the genes. Results: 174 DEGs between IBD and healthy controls were found from analyzing two datasets (GSE14580 and GSE73661), indicating a possible role in the pathogenesis of IBD. Of the 174 DEGs, five genes (SELE, TREM1, AQP9, FPR2, and HCAR3) were shared between all five datasets. Moreover, these five genes were identified as downregulated in the infliximab responder group compared to the non-responder group. Conclusions: We hypothesize that alteration in the expression of these genes leads to an impaired response to infliximab in IBD patients. Thus, these genes can serve as potential biomarkers for the early detection of compromised infliximab response in IBD patients.
Indirect Human Influences in Fear Landscapes: Varying Effects of Moonlight on Small Mammal Activity along Man-Made Gradients of Vegetation Structure
Authors: Pasquet Alba , Torre Ignasi , Díaz Mario
Published: March 2023

Risk of predation is one of the main constraints of small mammal distribution and foraging activity. Aside from numerical effects on population size due to the presence and abundance of predators, indirect cues, such as vegetation structure and moonlight, determine patterns of activity and microhabitat use by small mammals. Indirect cues are expected to interact, as shading provided by vegetation can suppress the effects of changing moonlight. We analyzed the effects of moonlight levels on the activity patterns of three common small mammal species in Mediterranean habitats, and tested whether moonlight effects were modulated by shadowing associated with the development of tall vegetation due to spontaneous afforestation following land abandonment. A. sylvaticus, a strictly nocturnal species, decreased activity under moonlight with no interactive effects of vegetation cover. C. russula showed no activity change with moonlight levels and M. spretus increased activity, although activity in both species was mostly determined by vegetation cover, that favored it. The effects of moonlight on small mammal activity were not homogeneous among species, nor were the interactive effects of man-made gradients of habitat structure, a fact that will produce community changes along vegetation gradients mediated by varying fear landscapes.
Drought Tolerance Assessment of Okra (Abelmoschus esculentus [L.] Moench) Accessions Based on Leaf Gas Exchange and Chlorophyll Fluorescence
Authors: Mkhabela Sonto Silindile , Shimelis Hussein , Gerrano Abe Shegro , Mashilo Jacob
Published: March 2023

Physiological and complementary phenotypic traits are essential in the selection of drought-adapted crop genotypes. Understanding the physiological response of diverse okra genotypes under drought stress conditions is critical to the selection of drought-tolerant accessions for production or breeding. The objective of this study was to assess the levels of drought tolerance in preliminarily selected okra accessions based on leaf gas exchange and chlorophyll fluorescence to determine best-performing genotypes for drought-tolerance breeding. Twenty-six genetically diverse okra accessions were screened under non-stressed (NS) and drought-stressed (DS) conditions under a controlled glasshouse environment using a 13 × 2 alpha lattice design in three replicates, in two growing seasons. Data were subjected to statistical analyses using various procedures. A significant genotype × water condition interaction effect was recorded for transpiration rate (T), net CO2 assimilation (A), intrinsic water use efficiency (WUEi), instantaneous water use efficiency (WUEins), minimum fluorescence (Fo′), maximum fluorescence (Fm′), maximum quantum efficiency of photosystem II photochemistry (Fv′/Fm′), the effective quantum efficiency of PSII photochemistry (ɸPSII), photochemical quenching (qP), nonphotochemical quenching (qN) and relative measure of electron transport to oxygen molecules (ETR/A). The results suggested variable drought tolerance of the studied okra accessions for selection. Seven principal components (PCs) contributing to 82% of the total variation for assessed physiological traits were identified under DS conditions. Leaf gas exchange parameters, T, A and WUEi, and chlorophyll fluorescence parameters such as the ɸPSII, Fv′/Fm′, qP, qN, ETR and ETR/A had high loading scores and correlated with WUEi, the ɸPSII, qP and ETR under DS conditions. The study found that optimal gas exchange and photoprotection enhance drought adaptation in the assessed okra genotypes and tested water regimes. Using the physiological variables, the study identified drought-tolerant accessions, namely LS05, LS06, LS07 and LS08 based on high A, T, Fm′, Fv′/Fm′ and ETR, and LS10, LS11, LS18 and LS23 based on high AES, Ci, Ci/Ca, WUEi, WUEins, ɸPSII and AES. The selected genotypes are high-yielding (≥5 g/plant) under drought stress conditions and will complement phenotypic data and guide breeding for water-limited agro-ecologies.
Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
Authors: Yang Wan-Wen , Hsu Chin-Wen , Chan Yu-Ju , Su Shih-Bin , Feng I-Jung , Hou Chia-Yi , Huang Chien-Yuan
Published: March 2023

This study aimed to compare the SARS-CoV-2 nucleic acid detection results of the BD MAX™ System and other platforms to formulate an optimized laboratory verification process. The re-examination of 400 samples determined as positive by BD MAX™ indicated that the inconsistency rate between BD MAX™ and the other platforms was 65.8%; the inconsistency rate of single-gene-positive results was as high as 99.2%. A receiver operating characteristic curve was drawn for the relative light unit (RLU) values of samples positive for a single gene, and RLU 800 was used as the cutoff. After setting the retest standard as single-gene positive and RLU ≥ 800, the number of the 260 BD MAX™ single-gene positives that needed to be confirmed again was 36 (13.8%) and the number that could be directly reported as negative was 224 (86.2%). This verification process can shorten the reporting period and speed up the epidemic adjustment time and turnover rate of special wards, thereby improving SARS-CoV-2 detection efficiency and clinical decision-making.
Abnormal Locations of Thymic Tissue as an Uncommon Cause of Neck Masses in Children: A Practical Approach
Authors: Andreozzi Laura , Sacchi Chiara , Biagi Carlotta , Dondi Arianna , Baldazzi Michelangelo , Carfagnini Filomena , Greco Laura , Vivacqua Donatella , Lanari Marcello
Published: March 2023

Background: The thymus gland is a lymphoid organ normally located in the anterior mediastinum. Location abnormalities of the thymus, such as ectopic thymus or the superior herniation of a mediastinal thymus, could be responsible for the occurrence of cervical masses in pediatric patients, raising concerns among clinicians. The knowledge of these conditions is essential for a thorough differential diagnosis and for preventing unnecessary invasive procedures. Methods: Descriptive retrospective series of three patients with a cervical mass, that was later diagnosed as ectopic thymic tissue. Results: The thymus has a unique and distinctive ultrasound appearance that is the keystone to detecting thymic parenchyma in locations other than anterior mediastinum. In selected patients, an accurate ultrasound could be conclusive for the diagnosis, with no need for further and potentially risky procedures. Conclusions: This case series supports the use of ultrasound in both diagnosis and follow-up of thymus location abnormalities, advocating a minimal invasive and conservative approach.
Characterization of AtBAG2 as a Novel Molecular Chaperone
Authors: Kang Chang Ho , Lee Jae Hyeok , Kim Yeon-Ju , Kim Cha Young , Lee Soo In , Hong Jong Chan , Lim Chae Oh
Published: March 2023

Bcl-2-associated anthanogene (BAG) family proteins regulate plant defense against biotic and abiotic stresses; however, the function and precise mechanism of action of each individual BAG protein are not yet clear. In this study, we investigated the biochemical and molecular functions of the Arabidopsis thaliana BAG2 (AtBAG2) protein, and elucidated its physiological role under stress conditions using mutant plants and transgenic yeast strains. The T-DNA insertion atbag2 mutant plants were highly susceptible to heat shock, whereas transgenic yeast strains ectopically expressing AtBAG2 exhibited outstanding thermotolerance. Moreover, a biochemical analysis of GST-fused recombinant proteins produced in bacteria revealed that AtBAG2 exhibits molecular chaperone activity, which could be attributed to its BAG domain. The relevance of the molecular chaperone function of AtBAG2 to the cellular heat stress response was confirmed using yeast transformants, and the experimental results showed that overexpression of the AtBAG2 sequence encoding only the BAG domain was sufficient to impart thermotolerance. Overall, these results suggest that the BAG domain-dependent molecular chaperone activity of AtBAG2 is indispensable for the heat stress response of Arabidopsis. This is the first report demonstrating the role of AtBAG2 as a sole molecular chaperone in Arabidopsis.
Human Neutrophil α-Defensins 1–3 Are Upregulated in the Microenvironment of Fibrotic Liver
Authors: Abu Fanne Rami , Maraga Emad , Kassem Eiass , Groisman Gabriel , Amsalem Naama , Zeina Abdel-Rauf , Abu Mouch Moran , Taher Randa , Abu-Mouch Saif
Published: March 2023

Background and Objectives: Neutrophil infiltration is an established signature of Non-Alcoholic Fatty Liver Disease (NAFLD) and Steatohepatitis (NASH). The most abundant neutrophilic peptide, alpha-defensin, is considered a new evolving risk factor in the inflammatory milieu, intimately involved in lipid mobilization. Our objective is to assess for potential association between alpha-defensin immunostains and NAFLD severity. Materials and Methods: We retrospectively investigated the liver biopsies of NAFLD/NASH patients, obtained at Hillel Yaffe Medical center between the years 2012 and 2016. Patients’ characteristics were recorded, including relevant blood tests at the time of biopsy. Each biopsy was semi-quantitatively scored using NAFLD Activity Score (NAS) and NASH fibrosis stage. The biopsies were immunostained for alpha-defensin. The precipitation of alpha-defensin was correlated to NAS and fibrosis. Results: A total of 80 biopsies were evaluated: male ratio 53.2%, mean age 44.9 ± 13.2 years, 54 had fibrosis grades 0–2, and 26 were grade 3–4. Conventional metabolic risk factors were more frequent in the high-grade fibrosis group. Immunostaining for alpha-defensin disclosed higher intensity (a.u.) in grade 3–4 fibrosis relative to grades 0–2, 25% vs. 6.5%, p < 0.05, respectively. Moreover, alpha-defensin staining was nicely co-localized with fibrosis. Conclusions: In our group of NASH/NAFLD patients, higher metabolic risk profile was associated with higher fibrosis grade. Immunostaining for alpha-defensin showed patchy intense staining concordant with high fibrosis, nicely co-localized with histological fibrosis. Whether alpha-defensin is a profibrotic risk factor or merely risk marker for fibrosis must be clarified in future studies.
Correlation between CT Abdominal Anthropometric Measurements and Liver Density in Individuals with Non-Alcoholic Fatty Liver Disease
Authors: Cucoranu Dragoș Constantin , Pop Marian , Niculescu Raluca , Vunvulea Vlad , Kosovski Irina-Bianca , Togănel Radu-Ovidiu , Russu Eliza , Mureșan Adrian Vasile , Licu Răzvan-Andrei , Bacârea Anca
Published: March 2023

Background: With a growing frequency, nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease worldwide. NAFLD has a strong correlation with other metabolic disorders, such as obesity, particularly abdominal obesity, even though the underlying causes or risk factors are not entirely understood. This study aims to investigate correlations between abdominal anthropometric measurements and the presence and intensity of liver steatosis as assessed by unenhanced computed tomography (CT). Methods: One hundred and nineteen patients (male/female, 66/53; mean age 54.54 +/− 12.90 years) underwent abdominal non–contrast-enhanced CT. CT images were examined to determine the attenuation of liver parenchyma, subcutaneous fat depth, and waist circumference (WC). Results: Among all patients, WC (r = −0.78, p < 0.0001), infraumbilical subcutaneous fat thicknesses (r = −0.51, p < 0.0001), right paraumbilical subcutaneous fat thicknesses (r = −0.62, p < 0.0001), and left paraumbilical subcutaneous fat thicknesses (r = −0.53, p < 0.0001) had a high inverse correlation with the liver attenuation values. The presence of T2D (OR: 2.40, p = 0.04), WC (OR: 11.45, p < 0.001), right paraumbilical (OR: 10.09, p < 0.001), left paraumbilical (OR: 2.81, p = 0.01), and infraumbilical (OR: 3.06, p = 0.007) were strongly independent predictors of NAFLD risk. Moreover, regarding the laboratory parameters, only the higher value of GGT (OR: 2.84, p = 0.009) is a predictor of NAFLD risk. Conclusions: Our data show that higher baseline values of all abdominal anthropometric measurements are correlated with liver attenuation and act as predictors of NAFLD risk.
Prevalence of Postoperative Atrial Fibrillation and Impact to Nursing Practice—A Cross Sectional Study
Authors: Brčina Ana , Civka Kristian , Habeković Renata , Krupa Sabina , Ljubas Ana , Mędrzycka-Dąbrowska Wioletta , Friganović Adriano
Published: March 2023

Background: Atrial fibrillation is the most common clinically significant cardiac arrhythmia, and it might lead to heart failure, which prolongs the duration of hospitalization and consequently increases the cost of treatment. Thus, diagnosing and treating atrial fibrillation should be the first line of defense against further complications. This study aimed to determine the incidence rate of postoperative atrial fibrillation and correlation with cardiac surgery on heart valves. A specific aim was to determine the relationship between the prevalence of atrial fibrillation and socio-demographic features. Methods: The study has a prospective cross-sectional design. The questionnaire was anonymous, requesting socio-demographic information as inclusion criteria, and the data were analyzed using descriptive statistics methods. Results: The sample was 201 patients. χ2 test and t-test were performed where we found that the frequency of atrial fibrillation was higher in the groups that have had valve surgery compared to other cardiac surgeries (χ2 = 7.695, ss = 2, p = 0.021). Atrial fibrillation increased with the age of the patients, but the prevalence of atrial fibrillation was not correlated with body weight. Conclusion: The results of this this study show that atrial fibrillation was higher in the participants who had valve surgery compared to other cardiac surgeries. There was also an increase in atrial fibrillation in the older participants. The results of this study can help to improve nursing practice and the quality of care for cardiac surgery patients with regard to daily activities, or planning nursing care due to the patient’s condition.
The Impact of the Minimum Ductility Requirement in Automotive Castings on the Carbon Dioxide Footprint throughout the Useful Life of an Electric Car
Authors: Tiryakioğlu Murat , Jarfors Anders E. W. , Leitner Martin
Published: March 2023

There is a trend in the automotive producers to require that foundries use more secondary aluminum alloy ingots to reduce the CO2 footprint of car production. The merits of this trend have been investigated in this study. Results have shown that requiring the use of more secondary ingots while simultaneously reducing the elongation requirement of aluminum alloy die castings is counterproductive, i.e., increases the CO2 footprint of the car over its useful life by not taking advantage of the weight reduction possible. It is recommended that (i) foundries improve their melt handling capabilities to reduce and minimize the entrainment damage made to the melt in the melting and casting process chain, and (ii) automobile producers reduce the weight of die castings by increasing requirements on elongation, to secure a reduced CO2-footprint in the designs, materials usage and life-cycle of cars.
Application of Magnetite-Nanoparticles and Microbial Fuel Cell on Anaerobic Digestion: Influence of External Resistance
Authors: Madondo Nhlanganiso Ivan , Rathilal Sudesh , Bakare Babatunde Femi , Tetteh Emmanuel Kweinor
Published: March 2023

In this paper, the application of magnetite-nanoparticles and a microbial fuel cell (MFC) was studied on the anaerobic digestion (AD) of sewage sludge. The experimental set-up included six 1 L biochemical methane potential (BMP) tests with different external resistors: (a) 100 Ω, (b) 300 Ω, (c) 500 Ω, (d) 800 Ω, (e) 1000 Ω, and (f) a control with no external resistor. The BMP tests were carried out using digesters with a working volume of 0.8 L fed with 0.5 L substrate, 0.3 L inoculum, and 0.53 g magnetite-nanoparticles. The results suggested that the ultimate biogas generation reached 692.7 mL/g VSfed in the 500 Ω digester, which was substantially greater than the 102.6 mL/g VSfed of the control. The electrochemical efficiency analysis also demonstrated higher coulombic efficiency (81.2%) and maximum power density (30.17 mW/ m2) for the 500 Ω digester. The digester also revealed a higher maximum voltage generation of 0.431 V, which was approximately 12.7 times the 0.034 V of the lowest-performing MFC (100 Ω digester). In terms of contaminants removed, the best-performing digester was the digester with 500 Ω, which reduced contaminants by more than 89% on COD, TS, VS, TSS and color. In terms of cost-benefit analysis, this digester produced the highest annual energy profit (48.22 ZAR/kWh or 3.45 USD/kWh). This infers the application of magnetite-nanoparticles and MFC on the AD of sewage sludge is very promising for biogas production. The digester with an external resistor of 500 Ω showed a high potential for use in bioelectrochemical biogas generation and contaminant removal for sewage sludge.
Quiescence of Escherichia coli Aerosols to Survive Mechanical Stress during High-Velocity Collection
Authors: Smith Brooke L. , King Maria D.
Published: March 2023

A low cutpoint wetted wall bioaerosol sampling cyclone (LCP-WWC), with an aerosol sampling flow rate of 300 L/min at 55″ H2O pressure drop and a continuous liquid outflow rate of about 0.2 mL/min, was developed by upgrading an existing system. The laboratory strain Escherichia coli MG1655 was aerosolized using a six-jet Collison Nebulizer and collected at high velocity using the LCP-WWC for 10 min with different collection liquids. Each sample was quantitated during a 15-day archiving period after aerosolization for culturable counts (CFUs) and gene copy numbers (GCNs) using microbial plating and whole-cell quantitative polymerase chain (qPCR) reaction. The samples were analyzed for protein composition and antimicrobial resistance using protein gel electrophoresis and disc diffusion susceptibility testing. Aerosolization and collection were followed by an initial period of quiescence or dormancy. After 2 days of archiving at 4 °C and RT, the bacteria exhibited increased culturability and antibiotic resistance (ABR), especially to cell wall inhibitors (ampicillin and cephalothin). The number of resistant bacteria on Day 2 increased nearly four-times compared to the number of cells at the initial time of collection. The mechanical stress of aerosolization and high-velocity sampling likely stunned the cells triggering a response of dormancy, though with continued synthesis of vital proteins for survival. This study shows that an increase in intensity in environmental conditions surrounding airborne bacteria affects their ability to grow and their potential to develop antimicrobial resistance.
Genomic Characterization and Wetland Occurrence of a Novel Campylobacter Isolate from Canada Geese
Authors: Linz David M. , McIntosh Kyle D. , Struewing Ian , Klemm Sara , McMinn Brian R. , Haugland Richard A. , Villegas Eric N. , Lu Jingrang
Published: March 2023

Populations of resident, non-migratory Canada geese are rapidly increasing. Canada geese are known to transmit viral and bacterial diseases, posing a possible threat to human health. The most prevalent pathogens vectored by geese are Campylobacter species, yet the current understanding of the identity and virulence of these pathogens is limited. In our previous study, we observed a high prevalence of Campylobacter spp. in the Banklick Creek wetland—a constructed treatment wetland (CTW) located in northern KY (USA) used to understand sources of fecal contamination originating from humans and waterfowl frequenting the area. To identify the types of Campylobacter spp. found contaminating the CTW, we performed genetic analyses of Campylobacter 16s ribosomal RNA amplified from CTW water samples and collected fecal material from birds frequenting those areas. Our results showed a high occurrence of a Campylobacter canadensis-like clade from the sampling sites. Whole-genome sequence analyses of an isolate from Canada goose fecal material, called MG1, were used to confirm the identity of the CTW isolates. Further, we examined the phylogenomic position, virulence gene content, and antimicrobial resistance gene profile of MG1. Lastly, we developed an MG1-specific real-time PCR assay and confirmed the presence of MG1 in Canada goose fecal samples surrounding the CTW. Our findings reveal that the Canada goose-vectored Campylobacter sp. MG1 is a novel isolate compared to C. canadensis that possesses possible zoonotic potential, which may be of human health concern.