bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementasi Augmented Reality Sebagai Media Pembelajaran Kata Benda Bahasa Arab pada Siswa Sekolah Dasar Islam Terpadu
Authors: Fatimah Dini Destiani Siti , Latifah Ayu , Haniyah Hani
Published: December 2022

Pesatnya perkembangan teknologi hingga detik ini membawa perkembangan serta kemajuan positif bagi dunia pendidikan dengan terciptanya berbagai media pembelajaran. Media pembelajaran yang beragam dan bisa dipakai dalam berbagai macam ilmu, salah satunya dalam ilmu bahasa. Ilmu bahasa dalam dunia pendidikan menjadi salah satu mata pelajaran yang penting, salah satunya mata pelajaran Bahasa Indonesia. Selain itu, ada beberapa bahasa lainnya yang wajib untuk dipelajari, salah satunya Bahasa Arab.Indonesia adalah salah satu Negara dengan jumlah muslim terbanyak di dunia, dan Bahasa Arab menjadi salah satu kurikulum pelajaran yang penting di bidang pendidikan Islam, seperti Pondok Pesantren atau sekolah berbasis Islam. Teknologi Augmented Reality merupakan teknologi yang mengambil dua aspek yaitu dunia nyata dan dunia maya, salah satu teknologi yang digunakan dalam AR ini adalah kamera disertai dengan penggunaan metode Multimedia Development Life Cycle terdiri dari enam tahap, yaitu Concept, Design, Material Collecting, Assembly, Testing, Distribution. Serta, dengan menggunakan beberapa alat dan bahan berupa Hardware ASUS A456U dan Samsung J6+ dengan software yang digunakan Unity. Dalam hal ini, pemanfaatan media pembelajaran dengan teknologi digital AR dibuat untuk para pelajar Sekolah Dasar Islam Terpadu, dimana di dalamnya terdapat mata pelajaran bahasa arab sebagai bahasa yang wajib dipelajari dan diketahui dalam proses KBM.
Penerapan Penerapan Data Mining Untuk Klasifikasi Kelulusan Mahasiswa Tepat Waktu Menggunakan Metode Random Forest
Authors: Oon Wira Yuda  , Darmawan Tuti  , Lim Sheih Yee  , Susanti
Published: December 2022

Salah satu faktor yang meningkatkan prestasi perguruan tinggi adalah kemampuan mahasiswa untuk menyelesaikan studi tepat waktu. Beberapa faktor dapat dilihat dari Indeks Prestasi Kumulatif (IPK) yang mencerminkan seluruh nilai yang diperoleh mahasiswa setiap semester yang sedang berjalan, dan jumlah SKS yang telah ditempuh mahasiswa. STMIK Amik Riau selalu berupaya untuk meningkatkan jumlah mahasiswa lulus tepat waktu secara terus menerus. Berdasarkan hal tersebut, maka dilakukan penelitian pada STMIK Amik Riau yang bertujuan untuk mengatasi masalah mahasiswa yang tidak lulus tepat waktu. Untuk mencapai tujuan tersebut, pada penelitian ini digunakan metode klasifikasi untuk mengklasifikasi kelulusan mahasiswa berdasarkan beberapa kategori yang telah ditentukan. Proses klasifikasi ini menggunakan algoritma Random Forest. Dengan menggunakan algoritma random forest ini dalam mengklasifikasi kelulusan mahasiswa, diperoleh tingkat akurasi sebesar 98%. Nilai akurasi yang diperoleh ini menunjukkan bahwa proses klasifikasi yang dilakukan sangat akurat.
BUSINESS INTELLIGENCE DATA MARKETING MENGUNAKAN METODE KIMBALL DAN ETL DENGAN POWER BI
Authors: Edhya Billah Faktha Putra
Published: December 2022

Dimasa ini perkembangan teknologi khususnya pada bidang perindustrian semakin pesat, hal tersebut dapat dilihat dari perkembangan teknologi pada bidang penjualan. Pada PT Dipa Pharmalab Intersains, setiap data penjualan disimpan dalam file Excel, yang mana pada aplikasi Excel ini hanya dapat menganalisa beberapa data, itupun tidak bisa dilakukan dengan cepat dan akurat. Dikarenakan aplikasi Excel tidak dapat menampilkan serta mengelompokan data yang ada di dalam perusahaan sesuai kategori dan kriteria perusahaan, maka hal tersebut membuat pengguna mengalami kesulitan untuk dapat menganalisis data penjualan yang dapat berguna untuk membantu perusahaan dalam pembuatan keputusan yang tepat dalam bisnis. Maka dari itu peneliti membuat sebuah sistem dengan menggunakan data warehouse dan aplikasi Power BI yang memiliki tujuan untuk dapat mempermudah proses analisis serta pengambilan keputusan bisnis yang tepat. Dalam penelitian ini menggunakan metode pemodelan data warehouse Kimballl 4 langkah, dimana terdiri dari beberapa proses tahapan antara lain adalah tahap ETL (Extract, Transform dan Load), lalu data yang ada akan diekstrak dalam bentuk tabel dimensi dan fakta, data dimensi dan faktra dapat dimasukan ke dalam MySQL menggunakan aplikasi Pentaho Data Integration, kemudian database akan diolah dan ditampilkan di aplikasi business intelligence Power BI. Pada tampilan Power BI pengguna dapat lebih mudah dalam melihat beberapa informasi yang dapat membantu perusahaan dalam menurunkan kerugian dalam proses produksi seperti informasi ketersediaan stok dan produk dapat dilihat dengan jelas dan dengan menggunakan aplikasi ini pengguna dapat melihat data produk terlaris dan pelanggan yang memiliki tingkat pembelian tertinggi.  Hal ini tentunya dapat membantu perusahaan dalam mengambil sebuah keputusan bisnis yang lebih akurat dan tepat.
SISTEM PENGENALAN WAJAH PADA KEAMAAN PINTU OTOMATIS MENGGUNAKAN METODE EIGENFACE BERBASIS RASPBERRY PI
Authors: Luriansyah Rizky Putra , Nugraha Danang Aditya , Harianto Wahyudi
Published: December 2022

Sistem pengenalan wajah pada keamanan pintu  otomatis sangat di perlukan bagi seseorang yang hendak menyimpan barang atau dokumen berharga supaya terhindar dari pencurian barang. Misalnya, pemilik rumah pribadi memiliki barang atau dokumen yang berharga dan pemilik rumah tersebut tidak ingin ada pihak lain yang mencuri barang tersebut maka diaplikasikan system pengenalan wajah pada pintu ruangan penyimpanan barang atau dokumen berharga di dalam rumah supaya hanya si pemilik yang dapat mengakses masuk ke dalam ruangan penyimpanan barang berharga tersebut. Implementasi system pengenalan wajah pada keamanan pintu menggunakan Raspberry pi 3 model B, sensor kamera, modul renal 5-volt, solenoid door lock. Berdasarkan hasil pengujian, system dapat bekerja dengan baik. System yang mendeteksi wajah juga dapat mengenali data sampel valid dan tidak valid dengan baik. Solenoid door lock juga akan membuka relay dengan otomatis jiga wajah yang terdeteksi valid, sebaliknya jika ada wajah yang tidak terdaftar di sampel wajah valid solenoid door lock tidak akan membuka relay tersebut.
DOKUMEN SOFTWARE REQUIREMENT SPESIFICATION (SRS) SISTEM INFORMASI PEMASARAN USAHA JASA PERCETAKAN DAN IKLAN
Authors: Serafintino Richi Andrew , Susilowati Meme
Published: December 2022

Usaha jada percetakan dan iklan ini merupakan sebuah perusahaan yang bergerak pada bidang digital marketing yang berlokasi di Kota Malang, Jawa Timur. Dikarenakan perusahaan tersebut belum menerapkan Sistem Informasi yang belum maksimal maka dibuatlah dokumenPerancangan Sistem Informasi Marketing ini dibuat dengan bertujuan untuk membuat sebuah dokumen SRS (Software Requirements Spesification) dengan standar ISO/IEC/IEEE 29148:2018. Dengan menggunakan metodologi 3 Major Phases untuk melakukan tahapan analisis dan desain. Hasil dari riset ini berupa dokumen SRS sistem informasi pemasaran (marketing) untuk menangani proses bisnis yang ada di usaha jasa percetakan dan iklan ini. Adapun SDD dilengkapi dengan workflow, diagram use case, aktivitas, requirement hingga logical database.
RANCANG BANGUN APLIKASI ANDROID UNTUK MINUM OBAT BAGI PASIEN KUSTA
Authors: Brylland Hartanto Calvin Septhanya , Tirma Irawan Paulus Lucky
Published: December 2022

Lepra atau kusta merupakan penyakit disebabkan oleh mycobacterium leprae yang menyerang jaringan tepi saraf dan dapat berimplikasi kepada pernafasan. Salah satu pengobatan yang dapat dilakukan adalah melakukan terapi minum obat antibiotik secara berkala selama 6 bulan hingga 2 tahun. Antibiotik dapat menghambat pertumbuhan dan mematikan bakteri sehingga jika tidak disiplin untuk meminum obat maka bakteri berduplikasi dan terapi obat dilakukan kembali dari awal. Ketidakdisiplinan penderita kusta dalam meminum obat disebabkan karena pasien lupa dalam meminum obat. Dikarenakan pasien kusta biasanya diisolasi, maka sulit bagi pengawas minum obat (PMO) untuk mengingatkan minum obat sehingga penelitian ini ditujukan untuk membuat aplikasi Android monitoring minum obat pasien kusta dan meneliti pengujian fungsional dan desainnya. Ken kerta, pendiri Lingkar Sosial, menuturkan bahwa selain dapat memonitor minum obat, aplikasi diharapkan dapat menampung data para pasien kusta agar dapat melakukan validasi banyak pasien yang terdapat pada suatu daerah dan volunteer yang tersedia. Perancangan aplikasi menggunakan usecase diagram, sequence diagram, class diagram, dan tabel relasi. Pengembangan aplikasi menggunakan bahasa pemrograman Java untuk sisi Android dan PHP untuk sisi back-end. Pengujian yang dilakukan menggunakan black box testing untuk uji fungsional fitur dan User experience quistionaire (UEQ) untuk uji keberhasilan desain. Fitur-fitur dari aplikasi berjalan dengan lancar berdasarkan hasil black box testing. HasilUEQ yang didapat, yaitu nilai attractiveness (Good), perspicuity (Above Average), efficiency (Good), dependability (Above Average), stimulation (Good), dan novelty (Above Average). Berdasarkan hasil UEQ, aplikasi mempunyai nilai tertinggi pada penilaian tingkat efisiensi, yaitu 1.75 yang artinya dinilai baik dalam efisiensi pemakaian dan praktis untuk digunakan.
MRI-guided stereotactic radiotherapy for localised prostate cancer
Authors:   , Willigenburg Thomas
Published: December 2022

Although the incidence of prostate cancer is high, improvements in treatments have led to high survival rates in patients with low- and intermediate-risk prostate cancer. Therefore, it is even more important that treatments for these patients are minimally invasive and cause as little side effects as possible. The last decades, radiotherapy treatments have changed significantly. One of the clinically introduced innovations is the MR-Linac, a combination of a linear accelerator and an MRI scanner. This machine allows high-quality imaging before and during treatment and daily treatment plan optimisation based on the changing anatomy. This enables more precise radiotherapy and therefore better sparing of healthy organs, which will potentially lead to less side effects such as urinary complaints and erectile dysfunction. This thesis provides insight into the clinical introduction and first period of prostate cancer treatment on the MR-Linac. In part 1 of this thesis, new workflows and technological developments for MRI-guided prostate cancer treatment are presented, which are aimed at enabling extremely hypofractionated radiotherapy for prostate cancer in one or two treatment fractions. With these new workflows, the irradiated volumes can be reduced significantly. Furthermore, early (patient-reported) outcomes of MRI-guided prostate cancer radiotherapy are investigated within two cohort studies, including the international MOMENTUM study. These clinical results are already promising. Part 2 of this thesis focusses on re-irradiation in patients with recurrent prostate cancer after primary radiotherapy treatment. We present research on the feasibility of so-called single-fraction ‘focal salvage’ radiotherapy using external beam radiotherapy on the MR-Linac. The work presented in this thesis is part of the first steps towards MRI-guided, extremely high-precision radiotherapy treatment for (recurrent) prostate cancer in only one or two treatment sessions.
Transcriptome-guided optimization of in vitro culture conditions to study microglia in health and disease
Authors:   , Timmerman Raissa
Published: December 2022

Neuroinflammation is a characteristic of almost all neurological diseases. Since microglia, the resident macrophages of the central nervous system (CNS), are key players in neuroinflammatory processes there is an increasing interest in this cell type as a therapeutical target to suppress neuroinflammation. Thorough biological knowledge of microglia is therefore of pivotal importance, and microglia in vitro models (in a culture dish) are excellent means to obtain such knowledge.

However, there is currently no in vitro microglia model that recapitulates all the characteristic features of in vivo microglia (in a living organism), which hampers the in vitro-in vivo translation. For instance, microglia in the healthy CNS are characterized by a ramified morphology, whereas in vitro microglia are characterized by a more amoeboid (rounder) morphology. In addition, the gene expression profile of in vitro microglia differs considerably from
the gene expression profile of ex vivo microglia (freshly isolated microglia).

Several studies have reported that cues from the CNS environment are important for microglia to establish or maintain their identity. However, which CNS environmental cues contribute to the in vivo microglia gene
expression profile that defines their identity is poorly understood. In this thesis, we used a transcriptomic-guided approach to uncover cues that shape microglia identity and investigated if they could be used to optimize in vitro culture conditions to study microglia in health and disease.

The results in this thesis have led to the development of a partly serum-free culture protocol that yields high numbers of ramified microglia with a transcriptome profile similar to that of microglia that were cultured in serum-free medium only. Additional transcriptome and in silico (computational) analyses throughout this thesis have provided powerful leads to further improve microglia in vitro culture conditions, for instance through i) the addition of intercellular microglia signaling cues, ii) the exposure to extracellular matrix components and iii) the generation of more biophysically accurate culture methods.

Better microglia in vitro models are pivotal to further understand the biology of microglia, and are also important to reduce and replace animal testing.
Ophthalmology Cystinosis Forum Afternoon Session
Authors: McEvoy Lynda
Published: December 2022

This 1-day meeting was held at the Austria Trend Savoyen Hotel, Vienna, Austria. The afternoon, chaired by Dr Hong Liang and Dr Rachel Bishop, consisted of a series of case presentations focussed on the treatment of ocular manifestations resultant of cystinosis, including the use of the novel gel-like formulation of cysteamine. Dr Inês Leal presented a series of four cases from the Hospital de Santa Maria, Lisbon, Portugal, illustrating the importance of early administration of treatment. Dr Giancarlo Iarossi presented a case highlighting the various examination techniques available to evaluate morphological and functional change during follow-up. Mr Susmito Biswas presented an interesting case of ocular cystinosis with concurrent congenital glaucoma, which brings a number of challenges. Dr Carla Ferreira presented two cases from the Hospital São João, Oporto, Portugal, that had two very different outcomes. Finally, Dr Eleonora Maria Soler Pava presented two cases from her clinic in Bogotá, Colombia, highlighting the good prognosis for patients when continual treatment is available.
Development of low-cost filters for the treatment of water polluted with
food waste leachate
Authors: Das Neelu , Srivastava Vivek , Goswami Rumi , Kumar Abhishek
Published: December 2022

This study investigates the efficacy of conventional and modified bio-sand filters as a low-cost filtering device for the treatment of groundwater contaminated with food waste leachate. Two kinds of waste materials, areca nut husk and water hyacinth stems, have been selected to alter the bio-sand filter. In conventional bio-sand filters, the ashes of these wastes are used as a filter medium. The ashes' physical characteristics, such as pH, ash content, and moisture content, have been measured. In leachate polluted water and purified water, tests for total solids, acidity, alkalinity, hardness, chloride content, pH, turbidity, dissolved oxygen, biological oxygen demand, and chemical oxygen demand have been performed. According to the testing findings, the developed filters are efficient in decreasing chloride, hardness, turbidity, chemical oxygen demand, and biological oxygen demand. The same filters have also detected an increase in dissolved oxygen. According to this study, these low-cost filters might be used as a preliminary treatment system for leachate created by food waste recycling plants.
Web 2.5 as a safe shift from Web 2.0 to Web 3.0: A definition of Web 2.5 in informatological approach
Authors: Gaweł Hanna
Published: December 2022

The blockchain ecosystem has undergone three distinct periods of exponential growth — the ICO boom of 2017, the Defi summer of 2020, and most recently the NFT wave of 2021.However, it was not yet seen a “web3-native” approach towards NFTs from any major brand. By that, we mean that no brand has upended its Web 2.0 architecture and replaced it entirely with a web3 stack. Instead, brands have taken a more measured approach, exploring NFT drops, integrations, and community growth developed separately from their core offerings. This phase can be described as Web 2.5. In this article, the author attempts to define what Web 2.5 is concerning informatology, along with identifying the potential future challenges of information management in this area and the new iteration of digital development, namely Web 3.0 or web3.
ANALYSIS OF HOMESTAY LODGING PROMOTION IN MANINJAU VILLAGE
Authors: Nabilla Almira Lamis  , Yuliana Yuliana , Ferdian Feri
Published: December 2022

Homestay in Maninjau Village is a simple type of lodging that only relies on a few rooms to be rented out to tourists and managed by the community. The purpose of this study is to analyze how homestay inn managers in Maninjau Village promote their lodging in terms of the media used and how homestay inn managers in Maninjau Village promote their lodging in terms of the manager's accuracy in choosing and using promotional media. Data collection techniques used are interviews, observation, and documentation. The informant determined in this study is Purpose Sampling, which is a technique of collecting data sources that are needed with certain considerations. The type of research used is descriptive with qualitative data consisting of five indicators, namely Advertising/Marketing, Personal Sales, Sales Promotion, Public Relations, and Word of Mouth. Data collection techniques used are interviews, observation, and documentation. It was concluded that there were two findings obtained. The general finding is that homestay managers in Maninjau Tourism Village have begun to understand that promotion is very important to increase homestay income. Managers have used advertising promotion media both in print media using business cards or relying on the internet. Managers are also starting to understand how they can promote homestays in a very simple way. Even so, there is a need for monitoring and increasing knowledge about promotion. Not only with hardcopy media and the internet, but promotions also require suggestions, comments, and criticisms to help improve homestay shortcomings and minimize the same mistakes in the future.
Determinants of Whistleblowing Within Local Government’s Internal Auditors
Authors: Ujang Hamdani
Published: December 2022

The effectiveness of whistleblowing as a part of organization’s internal control depends on the employee’s willingness to become whistleblowers. This research was conducted to examined effect of several individuals and contextual factors of local government’s internal auditors on the whistleblowing intention. Using the theory of planned behavior (TPB), the individual factors in this research were attitudes towards whistleblowing, subjective norms, and perceived whistleblowing control. The prosocial behavior theory was used to explain contextual factors such as independence commitment and public service motivation. Using stratified random sampling, we obtained 201 questionnaires from the population of the local government’s internal auditors in West Kalimantan Province. We used PLS-SEM with higher-order constructs to explain the relationship between components in the model. The research found that only three factors affected whistleblowing intentions: attitudes towards whistleblowing, subjective norms, and perceived whistleblowing control. The conclusion of this research indicates the importance of building an organizational ethical culture, designing definite reporting mechanisms, ensuring the trust of report follow-ups, and guaranteeing protection for whistleblowers.
Do hospital psiquiátrico à residência terapêutica
Authors: Borges Roselania Francisconi
Published: December 2022

Este artigo busca compreender os determinantes da vida de sujeitos denominados loucos que culminaram com sua institucionalização, bem como com sua inserção em residências terapêuticas. Instituídas pela Portaria GM nº 106/2000, as residências terapêuticas são uma das estratégias de desinstitucionalização que compõem a Rede de Atenção Psicossocial. Para além de sua definição enquanto moradias na comunidade cujo objetivo se dirige ao processo de reabilitação psicossocial para pessoas com “transtorno mental grave”, institucionalizadas ou não, a análise destes dispositivos pode elucidar paradoxos quanto aos atravessamentos de seus aspectos público e privado, às contradições inerentes ao modo de produção capitalista e ao paradigma psiquiátrico. Sendo um estudo de natureza exploratória qualitativa utiliza entrevistas semiestruturadas como meio de desvelar as perspectivas de seus atores sociais envolvidos: os moradores das residências terapêuticas. As discussões possibilitaram a reflexão crítica acerca dos determinantes sociais da experiência de sofrimento psíquico, a constatação de graves violações de direitos ainda existentes nos hospitais psiquiátricos e dos desafios e potencialidades da produção de cuidado e da vida em liberdade, sobretudo, num período marcado pelo desinvestimento na saúde pública e na atenção psicossocial perante o acirramento de políticas neoliberais
Perinatal exposure to foodborne inorganic nanoparticles: A role in the susceptibility to food allergy?
Authors: Issa Mohammad , Rivière Gilles , Houdeau Eric , Adel-Patient Karine
Published: December 2022

Graphical abstract
Identifying the key roles of a multicriteria framework for designing sustainable urban bridges in Ethiopia
Authors: Hailemariam Leule M. , Nuramo Denamo A.
Published: December 2022

This research investigates the role of using a multicriteria framework during the process of designing a sustainable urban bridge. A framework is a basic structure underlying a system, concept, or method. It is a hypothetical depiction of a complex entity or process. On the other hand, a multicriteria framework comprises several criteria or attributes that relate to the widespread usage of difficult-to-understand ideas, concepts, and approaches. The general purpose of such a framework is to make it easier to apply sustainable urban bridge design for infrastructure improvement. A literature review and a questionnaire survey are used in the study to identify the most important roles. The survey study, using a structured questionnaire, was applied to samples (n = 204) of practicing professionals in an urban bridge infrastructure design. A principal component analysis was carried out to pinpoint the key components. As a result, the multicriteria framework predominantly addresses sustainability principles in design practice and also guides and facilitates all bridge design processes, from conceptual analysis to final design outputs.
Regulation of bone health through physical exercise: Mechanisms and types
Authors: Chang Xinyu , Xu Sheng , Zhang Hao
Published: December 2022

Osteoporosis, characterized by bone mineral density reduction, bone mass loss, increased bone fragility, and propensity to fractures, is a common disease in older individuals and one of the most serious health problems worldwide. The imbalance between osteoblasts and osteoclasts results in the predominance of bone resorption and decreased bone formation. In recent years, it has been found that regular and proper exercise not only helps prevent the occurrence of osteoporosis but also adds benefits to osteoporosis therapy; accordingly, bone homeostasis is closely associated with mechanical stress and the intricate crosstalk between osteoblasts and osteoclasts. In this review, we summarize the mechanisms of exercise on osteoporosis and provide new proposals for the prevention and treatment of osteoporosis.
The causal relationship between obesity and skin and soft tissue infections: A two-sample Mendelian randomization study
Authors: Hu Hongxin , Mei Jian , Lin Mei , Wu Xianwei , Lin Haibin , Chen Guoli
Published: December 2022

Objective

Many observational studies have shown that obesity strongly affects skin and soft tissue infections (SSTIs). However, whether a causal genetic relationship exists between obesity and SSTIs is unclear.

Methods

A two-sample Mendelian randomization (MR) study was used to explore whether obesity is causally associated with SSTIs using a publicly released genome-wide association study (GWAS). An inverse-variance weighted (IVW) analysis was used as the primary analysis, and the results are reported as the odds ratios (ORs). Heterogeneity was tested using Cochran’s Q test and the I2 statistic, and horizontal pleiotropy was tested using the MR−Egger intercept and MR pleiotropy residual sum and outlier (MR-PRESSO).

Results

The results of the MR analysis showed a positive effect of BMI on SSTIs (OR 1.544, 95% CI 1.399-1.704, P= 5.86 × 10-18). After adjusting for the effect of type 2 diabetes (T2D) and peripheral vascular disease (PVD), the positive effect still existed. Then, we further assessed the effect of BMI on different types of SSTIs. The results showed that BMI caused an increased risk of impetigo, cutaneous abscess, furuncle and carbuncle, cellulitis, pilonidal cyst, and other local infections of skin and subcutaneous tissues, except for acute lymphadenitis. However, the associations disappeared after adjusting for the effect of T2D and PVD, and the associations between BMI and impetigo or cellulitis disappeared. Finally, we assessed the effects of several obesity-related characteristics on SSTIs. Waist circumference, hip circumference, body fat percentage, and whole-body fat mass, excluding waist-to-hip ratio, had a causal effect on an increased risk of SSTIs. However, the associations disappeared after adjusting for the effect of BMI.

Conclusion

This study found that obesity had a positive causal effect on SSTIs. Reasonable weight control is a possible way to reduce the occurrence of SSTIs, especially in patients undergoing surgery.
Pan-cancer analysis identifies DDX56 as a prognostic biomarker associated with immune infiltration and drug sensitivity
Authors: Ruan Zhaohui , Zhang Yuetong , Quan Qi , Jiang Jiaxin , Wang Qianyu , Zhang Yujing , Peng Roujun
Published: December 2022

DDX56, a member of the RNA helicase family, is upregulated in colon adenocarcinoma, lung squamous cell carcinoma, and osteosarcoma. However, the relationships between DDX56 and other tumors are not clear, and the molecular mechanism of its action is not fully understood. Here, we explore the biological functions of DDX56 in 31 solid tumors and clarify that DDX56 can promote oncogenesis and progression in multiple tumor types based on multi-omics data. Bioinformatics analysis revealed that the cancer-promoting effects of DDX56 were achieved by facilitating tumor cell proliferation, inhibiting apoptosis, inducing drug resistance, and influencing immune cell infiltration. Furthermore, we found that copy number alterations and low DNA methylation of DDX56 were likely to be related to aberrantly high DDX56 expression. Our results suggest that DDX56 is a potential pan-cancer biomarker that could be used to predict survival and response to therapy, as well as a potential novel therapeutic target. We validated some of our results and illustrated their reliability using CRISPR Screens data. In conclusion, our results clarify the role of DDX56 in the occurrence and development of multiple cancers and provide insight into the molecular mechanisms involved in the process of pathogenesis, indicating a direction for future research on DDX56 in cancers.
CMTM7 recognizes an immune-hot tumor microenvironment and predicts therapeutic response of immunotherapy in breast cancer well
Authors: Jiang Xingyu , Qian Zhengtao , Chen Yu , Zhou Tao , Zhao Can , Yin Yongxiang
Published: December 2022

Breast cancer (BRCA) is a complex disease that leads to major mortalities and unsatisfactory clinical outcomes among women worldwide. CKLF-like MARVEL transmembrane domain-containing 7 (CMTM7) is a potential tumor suppressor and regulator of PD-L1, which has been found as a functional signature in considerable oncogenesis, progression, and therapeutic resistance via deletion and downregulation. In this research, triple-negative breast cancer (BRCA), a molecular subtype having a lower response to endocrinotherapy but a higher response to chemotherapy and immunotherapy, showed higher transcriptional levels of CMTM7. Moreover, CMTM7 positively correlated with immunomodulators, tumor-infiltrating immune cells (TIICs), and immune checkpoints in many independent datasets. Furthermore, in an immunotherapy cohort of BRCA, patients with high CMTM7 expression were more sensitive to immunotherapy, and the therapeutic predictive value of CMTM7 is higher than that of PD-1 and PD-L1. To sum up, CMTM7 correlated with an inflamed tumor microenvironment and identified immune-hot tumors, which can be a novel biomarker for the recognition of immunological characteristics and an immunotherapeutic response in BRCA.
Review: Genetic and protein variants of milk caseins in goats
Authors: Rahmatalla Siham A. , Arends Danny , Brockmann Gudrun A.
Published: December 2022

The milk casein genes in goats, are highly polymorphic genes with numerous synonymous and non-synonymous mutations. So far, 20 protein variants have been reported in goats for alpha-S1-casein, eight for beta-casein, 14 for alpha-S2-casein, and 24 for kappa-casein. This review provides a comprehensive overview on identified milk casein protein variants in goat and non-coding DNA sequence variants with some affecting the expression of the casein genes. The high frequency of some casein protein variants in different goat breeds and geographical regions might reflect specific breeding goals with respect to milk processing characteristics, properties for human nutrition and health, or adaptation to the environment. Because protein names, alongside the discovery of protein variants, go through a historical process, we linked old protein names with new ones that reveal more genetic variability. The haplotypes across the cluster of the four genetically linked casein genes are recommended as a valuable genetic tool for discrimination between breeds, managing genetic diversity within and between goat populations, and breeding strategies. The enormous variation in the casein proteins and genes is crucial for producing milk and dairy products with different properties for human health and nutrition, and for genetic improvement depending on local breeding goals.
The study of neural antibodies in neurology: A practical summary
Authors: Fernández-Fournier Mireya , Lacruz Laura , Nozal Pilar , Chico Juan Luis , Tallón Barranco Antonio , Otero-Ortega Laura , Corral Iñigo , Carrasco Angela
Published: December 2022

The field of Autoimmune Neurology is expanding rapidly, with new neural antibodies being identified each year. However, these disorders remain rare. Deciding when to test for these antibodies, when and what samples are to be obtained, how to handle and study them correctly, and how to interpret test results, is complex. In this article we review current diagnostic techniques and provide a comprehensive explanation on the study of these patients, in an effort to help with correct diagnosis minimizing false positive and false negative results. We also propose routine storage of samples and referral of certain cases to specialized research laboratories.
Effect of inactivated COVID-19 vaccines on seizure frequency in patients with epilepsy: A multicenter, prospective study
Authors: Fang Xiqin , Hu Shimin , Han Tao , Yang Tingting , Hu Junji , Song Yucheng , Li Chunxiang , Ma Aihua , Li Yufeng , Kong Qingxia , Tang Liou , Chen Wei , Sun Wenxiu , Fang Chunyan , Sun Yanping , Chen Juan , Sun Wenying , Yan Yibing , Gao Yuxing , Geng Jianhong , Li Nan , Li Qiubo , Jiang Zhaolun , Lv Shishen , Li Wenke , Lang Xiaoling , Wang Suli , Chen Yanxiu , Li Baomin , Li Ling , Liu Xinjie , Liu Yong , Zhan Yan , Gao Zaifen , Qu Lixin , Fu Qingxi , Liu Xuewu
Published: December 2022

Objectives

Several COVID-19 vaccines list “uncontrolled epilepsy” as a contraindication for vaccination. This consequently restricts vaccination against COVID-19 in patients with epilepsy (PWE). However, there is no strong evidence that COVID-19 vaccination can exacerbate conditions in PWE. This study aims to determine the impact of COVID-19 vaccination on PWE.

Methods

PWE were prospectively recruited from 25 epilepsy centers. We recorded the seizure frequency at three time periods (one month before the first vaccination and one month after the first and second vaccinations). A generalized linear mixed-effects model (GLMM) was used for analysis, and the adjusted incidence rate ratio (AIRR) with 95% CI was presented and interpreted accordingly.

Results

Overall, 859 PWE were included in the analysis. Thirty-one (3.6%) and 35 (4.1%) patients were found to have increased seizure frequency after the two doses, respectively. Age had an interaction with time. The seizure frequency in adults decreased by 81% after the first dose (AIRR=0.19, 95% CI:0.11–0.34) and 85% after the second dose (AIRR=0.16, 95% CI:0.08–0.30). In juveniles (<18), it was 25% (AIRR=0.75, 95% CI:0.42–1.34) and 51% (AIRR=0.49, 95% CI:0.25–0.95), respectively. Interval between the last seizure before vaccination and the first dose of vaccination (ILSFV) had a significant effect on seizure frequency after vaccination. Seizure frequency in PWE with hereditary epilepsy after vaccination was significantly higher than that in PWE with unknown etiology (AIRR=1.95, 95% CI: 1.17–3.24). Two hundred and seventeen (25.3%) patients experienced non-epileptic but not serious adverse reactions.

Discussion

The inactivated COVID-19 vaccine does not significantly increase seizure frequency in PWE. The limitations of vaccination in PWE should focus on aspects other than control status. Juvenile PWE should be of greater concern after vaccination because they have lower safety. Finally, PWE should not reduce the dosage of anti-seizure medication during the peri-vaccination period.
Effect of dietary oregano oil on growth performance, disease resistance, intestinal morphology, immunity, and microbiota of hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)
Authors: Xin Yu , Liu Hao , Yan Xiaobo , Huang Weibin , Pan Simiao , Zhou Menglong , Lu Baiquan , Tan Beiping , Dong Xiaohui , Yang Yuanzhi
Published: December 2022

The study aimed to investigate the effects of oregano oil (OO) supplement in the diet on the growth performance, disease resistance, immunity, and intestinal health of hybrid grouper. Six isonitrogenous and isolipidic diets were fed to the grouper with an initial weight of 19.10 ± 0.12 g for 8 weeks, of which the dietary OO levels were 0 (Control), 0.1 (OO-0.1), 0.3 (OO-0.3), 0.6 (OO-0.6), 0.9 (OO-0.9), or 1.2 (OO-1.2) g kg-1. The results showed that there were no significant differences in growth performance among the six groups, while the OO supplement could improve the disease resistance ability of grouper. Our findings have demonstrated that the OO supplement, especially 0.3 g kg-1, could improve the intestinal morphology via increasing the villus number and muscle thickness and elevate digestive enzyme activities such as those of amylase (AMS) and lipase. The 0.3-g kg-1 OO supplement also can activate the antioxidant defense system, reduce nuclear factor erythroid 2-related factor 2-like 2 (Nrf2)/Kelch-like-ECH-associated protein 1 (Keap1) expression, and then enhance the immune ability via acid phosphatase (ACP), lysozyme (LYS), complement C3 (C3), and tight junction-associated signaling pathways in hybrid grouper. Meanwhile, the relative abundance of Firmicutes and Bacteroidetes in the OO-0.3 group was significantly higher than that of Control group (P< 0.05), while the relative abundance of Proteobacteria showed a basically opposite trend (P< 0.05). These findings suggested that dietary OO supplementation could significantly improve intestinal health and induce the antioxidant defense system and immunity, which means that OO could be used as a functional feed additive to protect the intestine, and the appropriate addition level of OO should be 0.3 g kg-1.
Gear fault detection in gearboxes operated in non-stationary conditions based on variable sideband analysis without a tachometer
Authors: Du Nguyen Trong , Dien Nguyen Phong , Nga Nguyen Thi Thanh
Published: December 2022

Machine fault diagnostic techniques by vibration signal analysis have been applied and widely developed in the industry. In recent years, using the least number of measuring devices to simplify the diagnostic process of gear fault has attracted many researchers. Various digital signal analysis methods in the time and frequency domains have been effectively applied to detect gear faults of gearboxes operated in stationary conditions or rotational speeds with minor fluctuations. However, in reality, gearboxes often work at rotational speeds with large fluctuations and variable loads due to technological progress. In such cases, the measured vibration signal is the amplitude–frequency modulated signal. Therefore, the traditional vibration analysis methods have become ineffective. The modulated signal analysis techniques often require additional key phase information measured using a tachometer to achieve good diagnostic results. However, setting up the tachometer while operating the machine is not effortless. This paper offers a new approach combining many methods to detect gear faults based on sideband analysis in the time–frequency distribution of measured vibration signals using only a single measurement channel. This study is applied in many cases where the machine cannot stop and reduce instrumentation requirements and can develop to build an online diagnostic system.
Alpha-peptide receptor radionuclide therapy using actinium-225 labeled somatostatin receptor agonists and antagonists
Authors: Shi Mengqi , Jakobsson Vivianne , Greifenstein Lukas , Khong Pek-Lan , Chen Xiaoyuan , Baum Richard P. , Zhang Jingjing
Published: December 2022

Peptide receptor radionuclide therapy (PRRT) has over the last two decades emerged as a very promising approach to treat neuroendocrine tumors (NETs) with rapidly expanding clinical applications. By chelating a radiometal to a somatostatin receptor (SSTR) ligand, radiation can be delivered to cancer cells with high precision. Unlike conventional external beam radiotherapy, PRRT utilizes primarily β or α radiation derived from nuclear decay, which causes damage to cancer cells in the immediate proximity by irreversible direct or indirect ionization of the cells’ DNA, which induces apoptosis. In addition, to avoid damage to surrounding normal cells, PRRT privileges the use of radionuclides that have little penetrating and more energetic (and thus more ionizing) radiations. To date, the most frequently radioisotopes are β– emitters, particularly Yttrium-90 (90Y) and Lutetium-177 (177Lu), labeled SSTR agonists. Current development of SSTR-targeting is triggering the shift from using SSTR agonists to antagonists for PRRT. Furthermore, targeted α-particle therapy (TAT), has attracted special attention for the treatment of tumors and offers an improved therapeutic option for patients resistant to conventional treatments or even beta-irradiation treatment. Due to its short range and high linear energy transfer (LET), α-particles significantly damage the targeted cancer cells while causing minimal cytotoxicity toward surrounding normal tissue. Actinium-225 (225Ac) has been developed into potent targeting drug constructs including somatostatin-receptor-based radiopharmaceuticals and is in early clinical use against multiple neuroendocrine tumor types. In this article, we give a review of preclinical and clinical applications of 225Ac-PRRT in NETs, discuss the strengths and challenges of 225Ac complexes being used in PRRT; and envision the prospect of 225Ac-PRRT as a future alternative in the treatment of NETs.
Combining omics tools for the characterization of the microbiota of diverse vinegars obtained by submerged culture: 16S rRNA amplicon sequencing and MALDI-TOF MS
Authors: Román-Camacho Juan J. , García-García Isidoro , Santos-Dueñas Inés M. , Ehrenreich Armin , Liebl Wolfgang , García-Martínez Teresa , Mauricio Juan C.
Published: December 2022

Vinegars elaborated in southern Spain are highly valued all over the world because of their exceptional organoleptic properties and high quality. Among the factors which influence the characteristics of the final industrial products, the composition of the microbiota responsible for the process and the raw material used as acetification substrate have a crucial role. The current state of knowledge shows that few microbial groups are usually present throughout acetification, mainly acetic acid bacteria (AAB), although other microorganisms, present in smaller proportions, may also affect the overall activity and behavior of the microbial community. In the present work, the composition of a starter microbiota propagated on and subsequently developing three acetification profiles on different raw materials, an alcohol wine medium and two other natural substrates (a craft beer and fine wine), was characterized and compared. For this purpose, two different “omics” tools were combined for the first time to study submerged vinegar production: 16S rRNA amplicon sequencing, a culture-independent technique, and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), a culture-dependent method. Analysis of the metagenome revealed numerous taxa from 30 different phyla and highlighted the importance of the AAB genus Komagataeibacter, which was much more frequent than the other taxa, and Acetobacter; interestingly, also archaea from the Nitrososphaeraceae family were detected by 16S rRNA amplicon sequencing. MALDI-TOF MS confirmed the presence of Komagataeibacter by the identification of K. intermedius. These tools allowed for identifying some taxonomic groups such as the bacteria genera Cetobacterium and Rhodobacter, the bacteria species Lysinibacillus fusiformis, and even archaea, never to date found in this medium. Definitely, the effect of the combination of these techniques has allowed first, to confirm the composition of the predominant microbiota obtained in our previous metaproteomics approaches; second, to identify the microbial community and discriminate specific species that can be cultivated under laboratory conditions; and third, to obtain new insights on the characterization of the acetification raw materials used. These first findings may contribute to improving the understanding of the microbial communities’ role in the vinegar-making industry.
Tubridge flow diverter alone vs. Tubridge flow diverter and coils for the treatment of intracranial aneurysms: A propensity score matching analysis
Authors: Shi Min , Feng Yu , Zhang Cheng-Da , Tang Qing-Wen , Li Ze-Jin , Zhao Wen-Yuan , Zhang Ting-Bao
Published: December 2022

Background

The study was designed to assess the clinical performance of a tubridge flow diverter (TFD) in the treatment of intracranial aneurysms and to compare the efficacy and safety between intracranial aneurysms treated with TFD alone and TFD combined with coiling.

Methods

In this retrospective study, patients treated with the TFD alone or TFD combined with coiling between June 2018 to November 2022 were included. The patient demographics, the characteristics of the aneurysm, and the treatment outcomes between the two groups were compared. Propensity score matching was performed to match the variables with a significant difference between groups.

Results

In the current study, data from 93 consecutive patients including 104 aneurysms treated with TFD were analyzed. In total, 43 patients with 49 aneurysms were treated with TFD alone, and 50 patients with 55 aneurysms were treated with TFD combined with coiling. Aneurysms in the TFD combined with the coiling group were larger (12.9 ± 8.6 vs. 8.7 ± 8.8 mm, P = 0.016) and more likely to be saccular (92.7% vs. 75.5%, P = 0.027) than in the TFD alone group. No significant difference was observed between the two groups in terms of perioperative complication rate. During the follow-up period, the complete occlusion rate in the TFD combined with the coiling group was higher (80.0% vs. 43.8%, P = 0.001) than in the TFD alone group. These results were further confirmed using a propensity score matching analysis.

Conclusion

TFD combined with coiling can be a safe and effective alternative option for the treatment of complex aneurysms. Given the potential risks of these therapeutic modalities, thus very careful consideration is required on an individual patient basis.
Lack of neural contributions to the summating potential in humans with Meniere’s disease
Authors: Riggs William J. , Fontenot Tatyana E. , Hiss Meghan M. , Varadarajan Varun , Moberly Aaron C. , Adunka Oliver F. , Fitzpatrick Douglas C.
Published: December 2022

Objective

To investigate the electrophysiology of the cochlear summating potential (SP) in patients with Meniere’s disease (MD). Although long considered a purely hair cell potential, recent studies show a neural contribution to the SP. Patients with MD have an enhanced SP compared to those without the disease. Consequently, this study was to determine if the enhancement of the SP was in whole or part due to neural dysfunction.

Design

Study participants included 41 adults with MD and 53 subjects with auditory neuropathy spectrum disorder (ANSD), undergoing surgery where the round window was accessible. ANSD is a condition with known neural dysfunction, and thus represents a control group for the study. The ANSD subjects and 17 of the MD subjects were undergoing cochlear implantation (CI) surgery; the remaining MD subjects were undergoing either endolymphatic sac decompression or labyrinthectomy to alleviate the symptoms of MD. Electrocochleography was recorded from the round window using high intensity (90 dB nHL) tone bursts. The SP and compound action potential (CAP) were measured to high frequencies (> = 2 kHz) and the SP, cochlear microphonic (CM) and auditory nerve neurophonic (ANN) to low frequencies. Linear mixed models were used to assess differences between MD and ANSD subjects.

Results

Across frequencies, the MD subjects had smaller alternating current (AC) response than the ANSD subjects (F = 31.61,534, p < 0.001), but the SP magnitudes were larger (F = 94.31,534, p < 0.001). For frequencies less than 4 kHz the SP magnitude in the MD group was significantly correlated with the magnitude of the CM (p’s < 0.001) but not in the ANSD group (p’s > 0.05). Finally, the relative proportions of both ANN and CAP were greater in MD compared to ANSD subjects. The shapes of the waveforms in the MD subjects showed the presence of multiple components contributing to the SP, including outer and inner hair cells and neural activity.

Conclusion

The results support the view that the increased negative polarity SP in MD subjects is due to a change in the operating point of hair cells rather than a loss of neural contribution. The steady-state SP to tones in human subjects is a mixture of different sources with different polarities.