bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeting autophagy in pancreatic cancer: The cancer stem cell perspective
Authors: Troumpoukis Dimitrios , Papadimitropoulou Adriana , Charalampous Chrysanthi , Kogionou Paraskevi , Palamaris Kostas , Sarantis Panagiotis , Serafimidis Ioannis
Published: November 2022

Pancreatic cancer is currently the seventh leading cause of cancer-related deaths worldwide, with the estimated death toll approaching half a million annually. Pancreatic ductal adenocarcinoma (PDAC) is the most common (>90% of cases) and most aggressive form of pancreatic cancer, with extremely poor prognosis and very low survival rates. PDAC is initiated by genetic alterations, usually in the oncogene KRAS and tumor suppressors CDKN2A, TP53 and SMAD4, which in turn affect a number of downstream signaling pathways that regulate important cellular processes. One of the processes critically altered is autophagy, the mechanism by which cells clear away and recycle impaired or dysfunctional organelles, protein aggregates and other unwanted components, in order to achieve homeostasis. Autophagy plays conflicting roles in PDAC and has been shown to act both as a positive effector, promoting the survival of pancreatic tumor-initiating cells, and as a negative effector, increasing cytotoxicity in uncontrollably expanding cells. Recent findings have highlighted the importance of cancer stem cells in PDAC initiation, progression and metastasis. Pancreatic cancer stem cells (PaCSCs) comprise a small subpopulation of the pancreatic tumor, characterized by cellular plasticity and the ability to self-renew, and autophagy has been recognised as a key process in PaCSC maintenance and function, simultaneously suggesting new strategies to achieve their selective elimination. In this review we evaluate recent literature that links autophagy with PaCSCs and PDAC, focusing our discussion on the therapeutic implications of pharmacologically targeting autophagy in PaCSCs, as a means to treat PDAC.
Cancer registration in the Middle East, North Africa, and Turkey (MENAT) region: A tale of conflict, challenges, and opportunities
Authors: Abdul-Sater Zahi , Mukherji Deborah , Adib Salim M. , Shamseddine Ali , Abu-Sitta Ghassan , Fadhil Ibtihal , Sullivan Richard , Omari Amal Al , Saleh Shadi , Taher Ali
Published: November 2022

Cancer registration is a core component of national and regional cancer control strategies. In the Middle East, North-Africa and Turkey (MENAT) region, capacity and resources for cancer registration is variable and shaped by multiple contextual challenges. This viewpoint maps out practical recommendations around cancer registration, in an attempt to inform cancer control planning, policy, and implementation. The recommendations laid out in this viewpoint are informed by the discussions held at the Initiative for Cancer Registration in the MENAT (ICRIM) virtual workshop, which convened registry managers, policy makers, and international agencies from 19 countries in the MENAT region. The discussions were distilled in four categories of recommendations, revolving around cancer registration procedures, collaborative governance, putting cancer registration on the map, and capacity building. This viewpoint provides a much-needed mapping of practical recommendations around cancer registration, informed by direct key stakeholders in the region. These practical recommendations offer a road map for policy making, cancer control planning, and future regional capacity strengthening initiatives.
The research progress of anti-inflammatory and anti-fibrosis treatment of chronic pancreatitis
Authors: Li Bing-Qing , Liu Xin-Yuan , Mao Tao , Zheng Tao-Hua , Zhang Peng , Zhang Qi , Zhang Yu , Li Xiao-Yu
Published: November 2022

Chronic pancreatitis (CP) is a chronic progressive inflammatory disease of the pancreas, caused by multiple factors and accompanied by irreversible impairment of pancreatic internal and external secretory functions. Pathologically, atrophy of the pancreatic acini, tissue fibrosis or calcification, focal edema, inflammation, and necrosis are observed. Clinical manifestations include recurrent or persistent abdominal pain, diarrhea, emaciation, and diabetes. In addition, CP is prone to develop into pancreatic cancer(PC) due to persistent inflammation and fibrosis. The disease course is prolonged and the clinical prognosis is poor. Currently, clinical treatment of CP is still based on symptomatic treatment and there is a lack of effective etiological treatment. Encouragingly, experiments have shown that a variety of active substances have great potential in the etiological treatment of chronic pancreatitis. In this paper, we will review the pathogenesis of CP, as well as the research progress on anti-inflammatory and anti-fibrotic therapies, which will provide new ideas for the development of subsequent clinical studies and formulation of effective treatment programs, and help prevent CP from developing into pancreatic cancer and reduce the prevalence of PC as much as possible.
Repeated trans-arterial treatments of LDL-DHA nanoparticles induce multiple pathways of tumor cell death in hepatocellular carcinoma bearing rats
Authors: Wang Yuzhu , Li Junjie , Vale Goncalo Dias do , Chaudhary Jaideep , Anwar Arnida , McDonald Jeffrey G. , Qin Tao , Zhang Hongwei , Corbin Ian R.
Published: November 2022

Introduction

Repeated hepatic arterial delivery of therapeutic agents to the liver by percutaneously implanted port-catheter systems has been widely used to treat unresectable liver cancer. This approach is applied to assess the therapeutic efficacy of repeated low-density lipoprotein-docosahexaenoic acid (LDL-DHA) nanoparticle treatments in a rat model of hepatocellular carcinoma.

Methods

N1S1 hepatoma bearing rats underwent placement of a percutaneously implanted hepatic artery port-catheter system and were allocated to untreated, control LDL-triolein (LDL-TO) or LDL-DHA nanoparticle infusions groups. Treatments were performed every three days over a nine day study period. MRI was performed at baseline and throughout the study. At the end of the study tissue samples were collected for analyses.

Results and Discussion

Implantation of the port catheters was successful in all rats. MRI showed that repeated infusions of LDL-DHA nanoparticles significantly impaired the growth of the rat hepatomas eventually leading to tumor regression. The tumors in the LDL-TO treated group showed delayed growth, while the untreated tumors grew steadily throughout the study. Histopathology and MRI support these findings demonstrating extensive tumor necrosis in LDL-DHA treated groups while the control groups displayed minor necrosis. Molecular and biochemical analyses also revealed that LDL-DHA treated tumors had increased levels of nuclear factor-kappa B and lipid peroxidation and depletion of glutathione peroxidase 4 relative to the control groups. Evidence of both ferroptosis and apoptosis tumor cell death was observed following LDL-DHA treatments. In conclusion repeated transarterial infusions of LDL-DHA nanoparticles provides sustained repression of tumor growth in a rat hepatoma model.
PET/CT radiomics for prediction of hyperprogression in metastatic melanoma patients treated with immune checkpoint inhibitors
Authors: Gabryś H. S. , Basler L. , Burgermeister S. , Hogan S. , Ahmadsei M. , Pavic M. , Bogowicz M. , Vuong D. , Tanadini-Lang S. , Förster R. , Kudura K. , Huellner M. , Dummer R. , Levesque M. P. , Guckenberger M.
Published: November 2022

Purpose

This study evaluated pretreatment 2[18F]fluoro-2-deoxy-D-glucose (FDG)-PET/CT-based radiomic signatures for prediction of hyperprogression in metastatic melanoma patients treated with immune checkpoint inhibition (ICI).

Material and method

Fifty-six consecutive metastatic melanoma patients treated with ICI and available imaging were included in the study and 330 metastatic lesions were individually, fully segmented on pre-treatment CT and FDG-PET imaging. Lesion hyperprogression (HPL) was defined as lesion progression according to RECIST 1.1 and doubling of tumor growth rate. Patient hyperprogression (PD-HPD) was defined as progressive disease (PD) according to RECIST 1.1 and presence of at least one HPL. Patient survival was evaluated with Kaplan-Meier curves. Mortality risk of PD-HPD status was assessed by estimation of hazard ratio (HR). Furthermore, we assessed with Fisher test and Mann-Whitney U test if demographic or treatment parameters were different between PD-HPD and the remaining patients. Pre-treatment PET/CT-based radiomic signatures were used to build models predicting HPL at three months after start of treatment. The models were internally validated with nested cross-validation. The performance metric was the area under receiver operating characteristic curve (AUC).

Results

PD-HPD patients constituted 57.1% of all PD patients. PD-HPD was negatively related to patient overall survival with HR=8.52 (95%CI 3.47-20.94). Sixty-nine lesions (20.9%) were identified as progressing at 3 months. Twenty-nine of these lesions were classified as hyperprogressive, thereby showing a HPL rate of 8.8%. CT-based, PET-based, and PET/CT-based models predicting HPL at three months after the start of treatment achieved testing AUC of 0.703 +/- 0.054, 0.516 +/- 0.061, and 0.704 +/- 0.070, respectively. The best performing models relied mostly on CT-based histogram features.

Conclusions

FDG-PET/CT-based radiomic signatures yield potential for pretreatment prediction of lesion hyperprogression, which may contribute to reducing the risk of delayed treatment adaptation in metastatic melanoma patients treated with ICI.
Epithelioid glioblastoma exhibits a heterogeneous molecular feature: A targeted next-generation sequencing study
Authors: Pan Rui , Wang Xiaotong , Fang Ru , Xia Qiuyuan , Wu Nan , Rao Qiu
Published: November 2022

Introduction

Epithelioid glioblastoma (eGBM) is one of the rare glioblastoma (GBM) variants in the current World Health Organization (WHO) categorization of central nervous system (CNS) tumours. However, the diagnostic basis and molecular features of eGBM have not been clearly defined to date. In this study, we aimed to molecularly characterize these tumours.

Methods

The clinicopathological, molecular, and immunohistochemical characteristics of 12 cases of eGBM were investigated.

Results

The tumours were found to be made up of epithelioid and rhabdoid cells when examined under a microscope. Six cases (50%) harboured the BRAF V600E mutation, and NF1 mutation was detected in 2 eGBM cases (16.7%). CDKN2A/B homozygous deletion was seen in 5 cases (41.7%). TP53 mutation was recognized in 2 instances (16.7%), and TERT promoter mutation was recognized in 5 cases (41.7%).

Discussion

eGBM is characterized by high molecular heterogeneity and has molecular overlaps between low-grade gliomas. Moreover, rather than being a variant or entity, the biological significance of the "epithelioid" appearance may be reduced to a simply morphological pattern. In order to target the proper treatment to suitable patients, molecular stratification via genome-wide molecular profiling will be crucial.
Association of neutrophil-lymphocyte ratio, platelet-lymphocyte ratio, and De Ritis ratio with mortality in renal cell carcinoma: A multicenter analysis
Authors: Keiner Cathrine , Meagher Margaret , Patil Dattatraya , Saito Kazutaka , Walia Arman , Liu Franklin , Dutt Raksha , Miller Nathan , Dhanji Sohail , Saidian Ava , Wan Fang , Yasuda Yosuke , Fujii Yasuhisa , Tanaka Hajime , Master Viraj , Derweesh Ithaar
Published: November 2022

Background

Several markers of inflammation have been associated with oncologic outcomes. Prognostic markers are not well-defined for renal cell carcinoma (RCC). We sought to investigate the association of preoperative neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), and De Ritis ratio with mortality in RCC.

Methods

Multi-center retrospective analysis of patients undergoing surgery for RCC. Primary outcome of interest was all-cause mortality (ACM). Secondary outcomes were non-cancer mortality (NCM) and cancer-specific mortality (CSM). Elevated NLR was defined as ≥2.27, elevated PLR as ≥165, and elevated De Ritis ratio as ≥ 2.72. Multivariable cox regression analysis (MVA) was conducted to elucidate risk factors for primary and secondary outcomes, and Kaplan-Meier analysis (KMA) was used to evaluate survival outcomes comparing elevated and non-elevated NLR, PLR, and De Ritis ratio.

Results

2656 patients were analyzed (874 patients had elevated NLR; 480 patients had elevated PLR and 932 patients had elevated De Ritis). Elevated NLR was a significant predictor of ACM (HR 1.32, 95% CI: 1.07-1.64, p=0.003) and NCM (HR 1.79, 95% CI: 1.30-2.46, p<0.001) in MVA. Elevated De Ritis was a significant predictor of ACM (HR 2.04, 95% CI: 1.65-2.52), NCM (HR 1.84, 95% CI: 1.33-2.55, p<0.001), and CSM (HR 1.97, 95% CI:1.48-2.63, p<0.001). KMA revealed significant difference in 5-year overall survival (OS) (48% vs. 68%, p<0.001), non-cancer survival (NCS) (69% vs. 87%, p<0.001), and cancer-specific survival (CSS) (60% vs. 73%, p<0.001) for elevated versus non-elevated NLR. For PLR, there was a difference in 5-year OS (51% vs. 61%, p<0.001) and CSS (60% vs. 73%, p<0.001) with KMA.

Conclusions

Elevated NLR was independently associated with worse ACM and NCM, while elevated De Ritis was predictive for CSM in addition to ACM and NCM. These differences may be useful in refining risk stratification with respect to cancer-related and non-cancer mortality in RCC patients and deserve further investigation.
Does the antimicrobial-impregnated peripherally inserted central catheter decrease the CLABSI rate in neonates? Results from a retrospective cohort study
Authors: Bayoumi Mohammad A. A. , van Rens Matheus F. P. T. , Chandra Prem , Masry Alaa , D’Souza Sunitha , Khalil Amr M. , Shadad Afaf , Alsayigh Safaa , Masri Razan M. , Shyam Sunitha , Alobaidan Fatima , Elmalik Einas E.
Published: November 2022

Background

The use of antimicrobial-impregnated peripherally inserted central catheters (PICCs) has been introduced in the last few years to neonatal units aiming to reduce central line-associated bloodstream infection (CLABSI).

Methods

This retrospective observational study aimed to compare the CLABSI rates and other catheter-related parameters including the insertion success rates and catheter-related complications in the antimicrobial-impregnated and conventional (ordinary) PICCs in NICU between 2017 and 2020.

Results

Our dedicated PICC team including physicians and nurses inserted 1,242 conventional (PremiCath and NutriLine) and 791 antimicrobial-impregnated PICCs (PremiStar) over the study period from 2017 to 2020. Of those 1,242 conventional PICCs, 1,171 (94.3%) were 1 Fr single lumen and only 71 (5.7%) were 2 Fr double lumen. The mean ± SD [median (IQR)] for the birth weight in all babies who had a PICC line was 1,343.3 ± 686.75 [1,200 (900, 1,500)] g, while the mean ± SD for the gestational age was 29.6 ± 4.03 [29 (27, 31)] weeks. The mean ± SD [median (IQR)] age at the time of insertion for all catheters was 9.3 ± 21.47 [2 (1, 9)] days, while the mean ± SD [median (IQR)] dwell time was 15.7 ± 14.03 [12 (8, 17)] days. The overall success rate of the PICC insertion is 1,815/2,033 (89.3%), while the first attempt success rate is 1,290/2,033 (63.5%). The mean ± SD [median (IQR)] gestational age, birth weight, age at catheter insertion, and catheter dwell time were 28.8 ± 3.24 [29, (26, 31)] weeks, 1,192.1 ± 410.3 [1,150, (900, 1,450)] g, 6.3 ± 10.85 [2, (1, 8)] days, and 17.73 ± 17.532 [13, (9, 18)] days in the antimicrobial-impregnated catheter compared with 30.1 ± 4.39 [29, (27, 32)] weeks (P < 0.001), 1,439.5 ± 800.8 [1,240, (920, 1,520)] g (P < 0.001), 11.1 ± 25.9 [1, (1, 9)] days (P < 0.001), and 14.30 ± 10.964 [12, (8, 17)] days (P < 0.001), respectively, in the conventional PICCs. The use of the antimicrobial-impregnated catheter was not associated with any significant reduction in the CLABSI rate (per 1,000 days dwell time), either the overall [P = 0.11, risk ratio (RR) (95% CI): 0.60 (0.32, 1.13)] or the yearly CLABSI rates.

Conclusions

The use of miconazole and rifampicin-impregnated PICCs did not reduce the CLABSI rate in neonates compared with conventional PICCs. However, it has a higher overall rate of elective removal after completion of therapy and less extravasation/infiltration, occlusion, and phlebitis compared with the conventional PICCs. Further large RCTs are recommended to enrich the current paucity of evidence and to reduce the risk of bias. Neonatal PICCs impregnation by other antimicrobials is a recommendation for vascular access device manufacturers.
Investigation of the allergens in 2,316 children with allergic rhinitis from Guangdong, China
Authors: Zhang Nannan , Wu Yunwen , Wei Zequn , Li Jinen , Shi Jiao , Cai Rong , Huang Hailing , Ouyang Siyuan , Zhang Qingfeng
Published: November 2022

Allergic rhinitis (AR) is one of the popular childhood diseases, bringing physical and metal burdens to the children and their families. The study was performed to detect common allergens eliciting AR in children, to investigate the prevalence of allergens in different age and gender cohorts, and to provide a reliable basis for clinical prevention and treatment of AR during childhood. We measured serum-specific IgE and performed inhalant and ingestion allergen examinations in 2,316 children with AR, in collaboration with BioSciTec GmbH. The prevalence of different allergens was determined according to gender, age, severity, and season. Among the 2,316 AR cases, the top five inhalant allergens were Dermatophagoides pteronyssinus (1,674 cases, 72.3%), Dermatophagoides farinae (1,520 cases, 65.6%), Blomia tropicalis (1,477 cases, 63.8%), Cockroach (602 cases, 26.0%), and Dog hair (602 cases, 26.0%). The top five ingestive allergens were Milk (1,111 cases, 48.0%), Egg white (543 cases, 23.4%), Shrimp/Crab (425 cases, 18.4%), Beef/Mutton (422 cases, 18.2%), and Egg yold (329 cases, 14.2%). AR severity analyses showed that 50.9% (1,180 cases) of D. pteronyssinus allergies were above level three, 47.9% (1,109 cases) of D. farinae allergies were above level three, only 23.3% (539 cases) of B. tropicalis allergies were level three, and B. tropicalis allergies were mainly of level 2. Other AR-inducing allergens mainly produced level one or two reactions. Regarding ingestion allergens, 7.9% (183 cases) of milk allergies and 4.7% (108 cases) of Shrimp/Crab allergies were above level three, and other allergens induced AR mainly of level one or two. The study investigated the major allergens eliciting AR in children from Guangdong, China, assessed the prevalence and severity among cohorts regarding age, gender, and season, and produced essential information on childhood AR, laying important references for AR prevention and treatment in the future.
Functions and clinical significance of circular RNAs in acute myeloid leukemia
Authors: Zhou Min , Gao Xianling , Zheng Xin , Luo Jing
Published: November 2022

Circular RNAs (circRNAs) are a class of covalently closed single-stranded RNA molecules. Four types of circRNAs have been reported in animal cells, and they have typical characteristics in their biogenesis, nuclear export and degradation. Advances in our understanding of the molecular functions of circRNAs in sponging microRNAs, modulating transcription, regulating RNA-binding proteins, as well as encoding proteins have been made very recently. Dysregulated circRNAs are associated with human diseases such as acute myeloid leukemia (AML). In this review, we focus on the recently described mechanisms, role and clinical significance of circRNAs in AML. Although great progress of circRNAs in AML has been achieved, substantial efforts are still required to explore whether circRNAs exert their biological function by other mechanisms such as regulation of gene transcription or serving as translation template in AML. It is also urgent that researchers study the machineries regulating circRNAs fate, the downstream effectors of circRNAs modulatory networks, and the clinical application of circRNAs in AML.
Analysis of a machine learning–based risk stratification scheme for acute kidney injury in vancomycin
Authors: Mu Fei , Cui Chen , Tang Meng , Guo Guiping , Zhang Haiyue , Ge Jie , Bai Yujia , Zhao Jinyi , Cao Shanshan , Wang Jingwen , Guan Yue
Published: November 2022

Vancomycin-associated acute kidney injury (AKI) continues to pose a major challenge to both patients and healthcare providers. The purpose of this study is to construct a machine learning framework for stratified predicting and interpreting vancomycin-associated AKI. Our study is a retrospective analysis of medical records of 724 patients who have received vancomycin therapy from 1 January 2015 through 30 September 2020. The basic clinical information, vancomycin dosage and days, comorbidities and medication, laboratory indicators of the patients were recorded. Machine learning algorithm of XGBoost was used to construct a series risk prediction model for vancomycin-associated AKI in different underlying diseases. The vast majority of sub-model performed best on the corresponding sub-dataset. Additionally, the aim of this study was to explain each model and to explore the influence of clinical variables on prediction. As the results of the analysis showed that in addition to the common indicators (serum creatinine and creatinine clearance rate), some other underappreciated indicators such as serum cystatin and cumulative days of vancomycin administration, weight and age, neutrophils and hemoglobin were the risk factors for cancer, diabetes mellitus, heptic insufficiency respectively. Stratified analysis of the comorbidities in patients with vancomycin-associated AKI further confirmed the necessity for different patient populations to be studied.
Using photonic reservoirs as preprocessors for deep neural networks
Authors: Bauwens Ian , Van der Sande Guy , Bienstman Peter , Verschaffelt Guy
Published: November 2022

Artificial neural networks are very time consuming and energy intensive to train, especially when increasing the size of the neural network in an attempt to improve the performance. In this paper, we propose to preprocess the input data of a deep neural network using a reservoir, which has originally been introduced in the framework of reservoir computing. The key idea of this paper is to use such a reservoir to transform the input data into a state in a higher dimensional state-space, which allows the deep neural network to process the data with improved performance. We focus on photonic reservoirs because of their fast computation times and low-energy consumption. Based on numerical simulations of delay-based reservoirs using a semiconductor laser, we show that using such preprocessed data results in an improved performance of deep neural networks. Furthermore, we show that we do not need to carefully fine-tune the parameters of the preprocessing reservoir.
Partial quantisation scheme for optimising the performance of hopfield network
Authors: Song Zhaoyang , Qu Yingjie , Li Ming , Liang Junqing , Ma Hongyang
Published: November 2022

The ideal Hopfield network would be able to remember information and recover the missing information based on what has been remembered. It is expected to have applications in areas such as associative memory, pattern recognition, optimisation computation, parallel implementation of VLSI and optical devices, but the lack of memory capacity and the tendency to generate pseudo-attractors make the network capable of handling only a very small amount of data. In order to make the network more widely used, we propose a scheme to optimise and improve its memory and resilience by introducing quantum perceptrons instead of Hebbian rules to complete its weight matrix design. Compared with the classical Hopfield network, our scheme increases the threshold of each node in the network while training the weights, and the memory space of the Hopfield network changes from being composed of the weight matrix only to being composed of the weight matrix and the threshold matrix together, resulting in a dimensional increase in the memory capacity of the network, which greatly solves the problem of the Hopfield network’s memory The problem of insufficient memory capacity and the tendency to generate pseudo-attractors was solved to a great extent. To verify the feasibility of the proposed scheme, we compare it with the classical Hopfield network in four different dimensions, namely, non-orthogonal simple matrix recovery, incomplete data recovery, memory capacity and model convergence speed. These experiments demonstrate that the improved Hopfield network with quantum perceptron has significant advantages over the classical Hopfield network in terms of memory capacity and recovery ability, which provides a possibility for practical application of the network.
Plant leaf veins coupling feature representation and measurement method based on DeepLabV3+
Authors: Liu Xiaobao , Xu Biao , Gu Wenjuan , Yin Yanchao , Wang Hongcheng
Published: November 2022

The plant leaf veins coupling feature representation and measurement method based on DeepLabV3+ is proposed to solve problems of slow segmentation, partial occlusion of leaf veins, and low measurement accuracy of leaf veins parameters. Firstly, to solve the problem of slow segmentation, the lightweight MobileNetV2 is selected as the extraction network for DeepLabV3+. On this basis, the Convex Hull-Scan method is applied to repair leaf veins. Subsequently, a refinement algorithm, Floodfill MorphologyEx Medianblur Morphological Skeleton (F-3MS), is proposed, reducing the burr phenomenon of leaf veins’ skeleton lines. Finally, leaf veins’ related parameters are measured. In this study, mean intersection over union (MIoU) and mean pixel accuracy (mPA) reach 81.50% and 92.89%, respectively, and the average segmentation speed reaches 9.81 frames per second. Furthermore, the network model parameters are compressed by 89.375%, down to 5.813M. Meanwhile, leaf veins’ length and width are measured, yielding an accuracy of 96.3642% and 96.1358%, respectively.
AMF colonization affects allelopathic effects of Zea mays L. root exudates and community structure of rhizosphere bacteria
Authors: Ma Junqing , Xie Yi , Yang Yisen , Jing Changliang , You Xiangwei , Yang Juan , Sun Chenyu , Qin Shengfeng , Chen Jianhua , Cao Kexin , Huang Jinghua , Li Yiqiang
Published: November 2022

Arbuscular mycorrhizal fungi (AMF) widely exist in the soil ecosystem. It has been confirmed that AMF can affect the root exudates of the host, but the chain reaction effect of changes in the root exudates has not been reported much. The change of soil microorganisms and soil enzyme vigor is a direct response to the change in the soil environment. Root exudates are an important carbon source for soil microorganisms. AMF colonization affects root exudates, which is bound to have a certain impact on soil microorganisms. This manuscript measured and analyzed the changes in root exudates and allelopathic effects of root exudates of maize after AMF colonization, as well as the enzymatic vigor and bacterial diversity of maize rhizosphere soil. The results showed that after AMF colonization, the contents of 35 compounds in maize root exudates were significantly different. The root exudates of maize can inhibit the seed germination and seedling growth of recipient plants, and AMF colonization can alleviate this situation. After AMF colonization, the comprehensive allelopathy indexes of maize root exudates on the growth of radish, cucumber, lettuce, pepper, and ryegrass seedlings decreased by 60.99%, 70.19%, 80.83%, 36.26% and 57.15% respectively. The root exudates of maize inhibited the growth of the mycelia of the pathogens of soil-borne diseases, and AMF colonization can strengthen this situation. After AMF colonization, the activities of dehydrogenase, sucrase, cellulase, polyphenol oxidase and neutral protein in maize rhizosphere soil increased significantly, while the bacterial diversity decreased but the bacterial abundance increased. This research can provide a theoretical basis for AMF to improve the stubble of maize and the intercropping mode between maize and other plants, and can also provide a reference for AMF to prevent soil-borne diseases in maize.
The Δ1-pyrroline-5-carboxylate synthetase family performs diverse physiological functions in stress responses in pear (Pyrus betulifolia)
Authors: Ma Changqing , Wang Mengqi , Zhao Mingrui , Yu Mengyuan , Zheng Xiaodong , Tian Yike , Sun Zhijuan , Liu Xiaoli , Wang Caihong
Published: November 2022

Δ1-Pyrroline-5-carboxylate synthetase (P5CS) acts as the rate-limiting enzyme in the biosynthesis of proline in plants. Although P5CS plays an essential role in plant responses to environmental stresses, its biological functions remain largely unclear in pear (Pyrus betulifolia). In the present study, 11 putative pear P5CSs (PbP5CSs) were identified by comprehensive bioinformatics analysis and classified into five subfamilies. Segmental and tandem duplications contributed to the expansion and evolution of the PbP5CS gene family. Various cis-acting elements associated with plant development, hormone responses, and/or stress responses were identified in the promoters of PbP5CS genes. To investigate the regulatory roles of PbP5CS genes in response to abiotic and biotic stresses, gene expression patterns in publicly available data were explored. The tissue-specific expressional dynamics of PbP5CS genes indicate potentially important roles in pear growth and development. Their spatiotemporal expression patterns suggest key functions in multiple environmental stress responses. Transcriptome and real-time quantitative PCR analyses revealed that most PbP5CS genes exhibited distinct expression patterns in response to drought, waterlogging, salinity-alkalinity, heat, cold, and infection by Alternaria alternate and Gymnosporangium haraeanum. The results provide insight into the versatile functions of the PbP5CS gene family in stress responses. The findings may assist further exploration of the physiological functions of PbP5CS genes for the development and enhancement of stress tolerance in pear and other fruits.
Continued spring phenological advance under global warming hiatus over the Pan-Third Pole
Authors: Yan Zhengjie , Xu Jinfeng , Wang Xiaoyi , Yang Zhiyong , Liu Dan , Li Guoshuai , Huang Huabing
Published: November 2022

The global surface temperature has witnessed a warming hiatus in the first decade of this century, but how this slowing down of warming will impact spring phenology over Pan-Third Pole remains unclear. Here, we combined multiple satellite-derived vegetation indices with eddy covariance datasets to evaluate the spatiotemporal changes in spring phenological changes over the Pan-Third Pole. We found that the spring phenology over Pan-Third Pole continues to advance at the rate of 4.8 days decade-1 during the warming hiatus period, which is contrasted to a non-significant change over the northern hemisphere. Such a significant and continued advance in spring phenology was mainly attributed to an increase in preseason minimum temperature and water availability. Moreover, there is an overall increasing importance of precipitation on changes in spring phenology during the last four decades. We further demonstrated that this increasingly negative correlation was also found across more than two-thirds of the dryland region, tentatively suggesting that spring phenological changes might shift from temperature to precipitation-controlled over the Pan-Third Pole in a warmer world.
Cultural factors and senior tourism: Evidence from the Chinese context
Authors: Tuo Jinmei , Deng Renli , Tao Ming , Xu Zucai , Peng Yan , Linghu Yushuang , Huang Shiming , Yu Changyin
Published: November 2022

Recently, numerous studies have focused on tourism among the older population. Of them, most reported on status analysis, tourism motivation, and tourism model, to name a few; however, there was a lack of comprehensive synthesis and analysis of the motivation, influencing factors, policy impact, and other factors of older tourism. Thus, this study conducted various keyword searches among both English and Chinese publications. We found that older population’s tourism is affected by various factors, such as travel expense, physical condition, the length and distance of a trip, and cultural influence. The results provide a reference for the development and implementation of tourism among the older population.
Parents' intentions toward preschool children's myopia preventive behaviors: Combining the health belief model and the theory of planned behavior
Authors: Jiang Nan , Chen Jiayue , Cao He , Liu Yongyi , Zhang Yuxin , Wang Quqin , Wang Ting , Zhao Huilan , Lu Hui , Yang Lei , Wang Jiwei
Published: November 2022

Objective

This study aimed to develop an integrated model based on the health belief model (HBM) and the theory of planned behavior (TPB) to explore the influencing factors of parents' intentions toward preschool children's myopia preventive behaviors.

Methods

This cross-sectional study was conducted in Minhang District, Shanghai, China in January 2022. One thousand six hundred and twenty-eight parents of preschool children from seven preschools were recruited in the study. A four-part questionnaire was used to collect data on socio-demographic characteristics, HBM variables, TPB variables and parental intentions. This study used exploratory factor analysis to analyze HBM and TPB items. Hierarchical multiple regression analysis was performed to explore the relationship between independent variables and parents' intentions toward preschool children's myopia preventive behaviors.

Results

The final integrative model showed that perceived severity, perceived barriers, attitudes, subjective norms, and perceived behavioral control were associated with parents' intentions toward preschool children's myopia preventive behaviors. In model 1, Child's age was entered as a control variable and explained 0.6% of the variance (F = 7.241, p = 0.007). When the HBM variables were entered in model 2, the proportion of variance increased to 25.4% (F = 73.290, P < 0.001). In model 3, TPB variables were entered and explained 63.2% of the variance (F = 246.076, p < 0.001).

Conclusion

The integrated model of HBM and TPB constructed in this study significantly improved the degree of explanation of parents' intentions toward preschool children's myopia preventive behaviors. Parents' perceived severity, perceived barriers, attitudes, subjective norms, and perceived behavioral control can be prioritized intervention targets for myopia preventive practices in preschool children.
Spatio-temporal effect of provincial technological innovation on environmental pollution in China
Authors: Wang Chu , Guo Xiaomin
Published: November 2022

The relationship between technological innovation (TL) and environmental pollution (EP) and its action mechanisms are complex and controversial aspects of discussion. Using the spatial autocorrelation analysis, standard deviation ellipse analysis, kernel density function, spatial econometric model, this study analyzed the spatial distribution, evolution characteristics, and influencing factors of the EP and TL from 2000 to 2020 in China. Results found there was a significant spatial autocorrelation between the EP and TL in 2000–2020. The standard deviation ellipse of EP was broadly distributed in the “southwest-northeast” direction, indicating that EP presented a trend of concentration in the direction of “southwest-northeast.” The moving trajectory of the center of gravity for the EP in 2000–2020 was essentially moved from the northeast to southwest. Overall, the national level of TL exhibited a “north-south change, high in the east, and low in the west” trend. Regional differences were gradually expanding, and the polarization was evident. Regardless of using least squares method (OLS) or quantile regression (QR) models, TL, human capital (HC), and industrial structure (IS) all had an inhibitory effect on the EP at the effective significance level. Total population (TP), foreign direct investment (FDI), and local fiscal expenditure (LFE) were positively related to the EP.
The evolution of mental health related policies in China: A bibliometric analysis, 1987–2020
Authors: Li Haiyan , Zhou Qingyu , Zhu Hao , Shi Peiwu , Shen Qunhong , Zhang Zhaoyang , Chen Zheng , Pu Chuan , Xu Lingzhong , Hu Zhi , Ma Anning , Gong Zhaohui , Xu Tianqiang , Wang Panshi , Wang Hua , Hao Chao , Li Chengyue , Hao Mo
Published: November 2022

Background

Since 1987, the Chinese government has promoted public mental health by continuously implementing mental health related policies. This research attempts to reveal the distribution and characteristics of mental health related policies. In addition, it can help stakeholders evaluate whether the environment for policy implementation has improved and identify key points in the development of the overall mental health system.

Methods

We used a bibliometric approach to analyze the evolution of mental health related policies in China from 1987 to 2020. A total of 239 mental health related policies were collected from Beida Fabao and official Internet websites of governmental departments. Co-wording, social networks, and citation analysis were applied to explore the evolutionary features of such policies.

Results

The evolution of policy development showed that the number of mental health related policies in China has been increasing and their content has been enriched. Over time, mental health related policies not only gradually expanded its focus on common mental disorders, but also included an increasing number of keywords related to service provision, organization and administration. However, most policies were implemented independently by separate agencies and the number of policies jointly implemented by different agencies only accounted for 32.64% of all the policies implemented. The Ministry of Health (MOH) is at the core of the collaborative network associated with implementing mental health related policies in China.

Conclusion

The environment associated with the implementation of mental health related policies in China is gradually improving. However, cross-sector collaboration among different agencies needs to be strengthened and financial support for related resources needs more attention. A clear division of responsibilities among various agencies and a sustainable financing mechanism are essential to the development and implementation of mental health related policies.
Relation between magnetosonic waves and pitch angle anisotropy of warm protons
Authors: Joseph J. , Jaynes A. N. , Ma Q. , Hartley D. P. , Usanova M. E. , Li W.
Published: November 2022

In the past decade, many observations of transversely heated low energy protons were reported in the inner magnetosphere. Interestingly, most of the time heated protons were observed along with magnetosonic waves. Due to the strong correlation, it was often assumed that magnetosonic waves were responsible for the heating of low energy protons. By performing a case study under unusually disturbed geomagnetic conditions, this paper unravels the controversial relationship between the observed pitch angle anisotropy of warm protons and the accompanying magnetosonic waves in the inner magnetosphere. We perform a comparative analysis involving two nearly identical cases of pitch angle anisotropy of warm protons in low L-shell region–one with magnetosonic waves and one without them. It is found that magnetosonic waves are not responsible for primary heating of low-energy protons and may just marginally alter the shape of the distribution of heated protons in the events analyzed. Based on the recent Cluster and POLAR observations, we also show how the recirculated polar wind plasma in the Earth’s magnetosphere can cause the concurrent appearance of heated protons and magnetosonic waves.
Systematic review and meta-analysis of the growth performance and carcass composition of Nile tilapia (Oreochromis niloticus) fed dietary copra meal
Authors: Magbanua Tzodoq Obrero , Ragaza Janice Alano
Published: November 2022

Introduction

Nile tilapia (Oreochromis niloticus) is one of the most cultured species in the aquaculture industry. However, its market price is affected by the increasing cost of fishmeal. Plant-based proteins are being used to mitigate the problem.

Methodology

This paper comprehensively quantified the growth performance and carcass composition of Nile tilapia fed copra mealviasystematic review and meta-analysis. The literature review and paper selection were done based on the preferred reporting items for systematic reviews and meta-analysis method. The data from peer-reviewed papers that qualified from the inclusion screening criteria such as standard error reporting, feeding period, absence of dietary supplements, tilapia development stage, and crude protein levels were subjected to meta-analysis.

Results

Results showed that growth and feed utilization parameters were significantly different (P≤ 0.05), favoring the control over the plant-based diets. Moreover, the pooled estimate of carcass composition of fish fed copra meal-based diet was not significantly different with that of fish fed fishmeal-based diet (P> 0.05) except for ash content (P< 0.05). The feeding period, crude protein level, fish development stage, and dietary copra inclusions were subjected to meta-regression analysis due to high and statistically significant I2values to determine the sources of heterogeneity. The residual I2values showed that these factors did not affect the heterogeneity of the data presented for copra meal. These effects may be attributed to the nutritional quality of raw materials and other abiotic factors like feed preparation.

Conclusion

Nonetheless, data from meta-analysis using model coefficients revealed that the minimum required levels are 594, 617, and 462 g kg−1of dietary copra meal are needed for optimum growth performance, feed utilization, and carcass composition of Nile tilapia, respectively. The study showed that systematic review and meta-analysis can be a useful tool in optimizing plant-based diets for aquaculture species.
Analysis of reproduction-related transcriptomes on pineal-hypothalamic-pituitary-ovarian tissues during estrus and anestrus in Tan sheep
Authors: Wei Shihao , Kang Xiaolong , Yang Chaoyun , Wang Feng , Dai Tianshu , Guo Xingru , Ma Ziming , Li Chenglong , Zhao Hongxi , Dan Xingang
Published: November 2022

Seasonal estrus is an important factor limiting the fertility of some animals such as sheep. Promoting estrus in the anestrus season is one of the major ways in improving the fecundity of seasonally breeding animals. The pineal-hypothalamus-pituitary-ovary (PHPO) axis plays a decisive role in regulating animal reproduction. However, the molecular mechanisms by which the PHPO axis regulates seasonal reproduction in animals are not well understood, especially in Tan sheep. To this end, we collected pineal, hypothalamus, pituitary and ovary tissues from Tan sheep during estrus and anestrus for RNA-Sequencing, and performed bioinformatics analysis on the entire regulatory axis of the pineal-hypothalamic-pituitary-ovary (PHPO). The results showed that 940, 1,638, 750, and 971 DEGs (differentially expressed genes, DEGs) were identified in pineal, hypothalamus, pituitary and ovary, respectively. GO analysis showed that DEGs from PHPO axis-related tissues were mainly enriched in “biological processes” such as transmembrane transport, peptide and amide biosynthesis and DNA synthesis. Meanwhile, KEGG enrichment analysis showed that the bile acid secretion pathway and the neuroactive ligand-receptor interaction pathway were significantly enriched. Additionally, four potential candidate genes related to seasonal reproduction (VEGFA, CDC20, ASPM, and PLCG2) were identified by gene expression profiling and protein-protein interaction (PPI) analysis. These findings will contribute to be better understanding of seasonal reproduction regulation in Tan sheep and will serve as a useful reference for molecular breeding of high fertility Tan sheep.
Impact of multi-drug resistance on clinical outcomes of dogs with corneal ulcers infected with Staphylococcus pseudintermedius
Authors: Mauer Ashley N. , Allbaugh Rachel A. , Kreuder Amanda J. , Sebbag Lionel
Published: November 2022

Objective

Compare characteristics and clinical outcomes of dogs with infectious keratitis from Staphylococcus pseudintermedius considered to be multidrug-resistant (MDR) or not.

Procedures

Staphylococcus pseudintermedius isolated as the primary pathogen from canine patients with ulcerative keratitis were considered MDR if resistant to at least one agent in three or more classes of antibiotics. Medical records were reviewed for history, patients' characteristics, clinical appearance, therapeutic interventions, and clinical outcomes.

Results

Twenty-eight dogs (28 eyes) were included. Compared to non-MDR cases, MDR diagnosis was significantly more common in dogs with recent (≤30 days) anesthesia (7/15 vs. 1/13, P = 0.038) and more common in non-brachycephalic dogs (8/15 vs. 2/13, P = 0.055). Clinical appearance (ulcer size/depth, anterior chamber reaction, etc.) did not differ significantly between groups (P ≥ 0.055). Median (range) time to re-epithelialization was longer in MDR vs. non-MDR eyes [29 (10–47) vs. 22 (7–42) days] but the difference was not significant (P = 0.301). Follow-up time was significantly longer in dogs with MDR keratitis [47 (29–590) vs. 29 (13–148) days, P = 0.009]. No other significant differences were noted between MDR and non-MDR eyes in regard to time for ulcer stabilization [4 (1–17) days vs. 4 (1–12), P = 0.699], number of eyes requiring surgical stabilization (7/15 vs. 7/13, P = 0.246) or enucleation (1/15 vs. 2/13, P = 1.000), success in maintaining globe (14/15 vs. 11/13, P = 0.583) or success in maintaining vision (12/15 vs. 10/13, P = 1.000).

Conclusions

MDR infections may prolong corneal healing time but did not appear to affect overall clinical outcomes in dogs with bacterial keratitis. Further research is warranted in a larger canine population and other bacterial species.
Patterns of mortality in domesticated ruminants in Ethiopia
Authors: Innocent Giles T. , Vance Ciara , Ewing David A. , McKendrick Iain J. , Hailemariam Solomon , Nwankpa Veronica R. , Allan Fiona K. , Schnier Christian , Peters Andrew R.
Published: November 2022

Background

Premature death of livestock is a problem in all ruminant production systems. While the number of premature ruminant deaths in a country is a reasonable indicator for the nation's health, few data sources exist in a country like Ethiopia that can be used to generate valid estimates. The present study aimed to establish if three different data sets, each with imperfect information on ruminant mortality, including abortions, could be combined into improved estimates of nationwide mortality in Ethiopia.

Methods

We combined information from a recent survey of ruminant mortality with information from the Living Standards Measurement Study and the Disease Outbreak and Vaccination Reporting dataset. Generalized linear mixed and hurdle models were used for data analysis, with results summarized using predicted outcomes.

Results

Analyses indicated that most herds experienced zero mortality and reproductive losses, with rare occasions of larger losses. Diseases causing deaths varied greatly both geographically and over time. There was little agreement between the different datasets. While the models aid the understanding of patterns of mortality and reproductive losses, the degree of variation observed limited the predictive scope.

Conclusions

The models revealed some insight into why mortality rates are variable over time and are therefore less useful in measuring production or health status, and it is suggested that alternative measures of productivity, such as number of offspring raised to 1 year old per dam, would be more stable over time and likely more indicative.