bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Studies concerning Selected Properties of a Composite Material Blended with Nanofluoroapatite Crystals
Authors: Zietek Marta , Dobrzynski Maciej , Fita Katarzyna , Diakowska Dorota , Watras Adam , Wiglusz Rafal Jakub
Published: December 2021

The aim of the paper was to determine the potential for fluorine release from an original composite material blended with nanofluoroapatite (FAp). The level of fluoride ion emission into deionized water and saline was studied over a period of 12 weeks. Values were recorded after 1, 3, 24, 48, 72, and 96 h and then weekly for a period of 12 weeks. There were statistically significant differences in the periods of fluoride ion release from 5%FAp and 2%FAp materials into saline solution as well as into deionized water. The highest fluorine release from 5%FAp + polymer was observed in the 10th and 11th week of incubation (for saline solution) and in the 9th, 10th, and 11th week (for deionized water). The highest fluorine release from 2%FAp + polymer was observed in the 9th, 11th, and 12th week of incubation for both environments. Total fluoride ion release from 5%FAp + polymer and mean fluoride release levels were similar in 5%FAp and 2%FAp in both environments. Both tested materials (5%FAp and 2%FAp) show the ability to release fluoride ions over a long time in the experimental environment.
Broadband Bi-Directional Polarization-Insensitive Metamaterial Absorber
Authors: Tian Feng , Ma Xia , Hao Han , Li Xuewen , Fan Jingdao , Guo Liang , Huang Xiaojun
Published: December 2021

Conventional metamaterial absorbers eliminate the transmitted electromagnetic wave by attaching the metal plate with the unidirectional absorption performance; these absorbers limit the practical applications to a large extent. In this paper, we present a broadband bi-directional metamaterial absorber by etching chip resistors on the resonators for expanding the bandwidth, and two orthogonal I-shaped structures are pasted on the both sides of the ultra-thin substrate (FR-4) instead of the metal plate for enhancing absorptance of the absorber. Simulated results show that absorptance of the designed absorber is larger than 0.9 in 1.43–2.51 GHz along the forward and backward directions under both TE and TM polarizations. Microwave experiments in the chamber are performed to verify the simulations, and the experimental results exhibit the excellent agreement with the simulations. Additionally, two I-shaped structures are orthogonally pasted on an ultrathin substrate, leading to the impedance-matching of both forward and backward directions, and the absorptance can be tailed dynamically via the middle layer of the substrate. The physics of the absorption are visualized by using a transmission line based on equivalent circuits. We claim that the designed bi-directional metamaterial absorber can be a good candidate for electromagnetic stealth and energy harvesting.
3D Multi-Track and Multi-Layer Epitaxy Grain Growth Simulations of Selective Laser Melting
Authors: Dezfoli Amir Reza Ansari , Lo Yu-Lung , Raza M. Mohsin
Published: December 2021

An integrated simulation framework consisting of the 3D finite element method and 3D cellular automaton method is presented for simulating the multi-track and multi-layer selective laser melting (SLM) process. The framework takes account of all the major multi-physics phenomena in the SLM process, including the initial grain structure, the growth kinetics, the laser scanning strategy, the laser–powder and laser–matter interactions, the melt flow, and the powder-to-liquid-to-solid transformations. The feasibility of the proposed framework is demonstrated by simulating the evolution of the epitaxy grain structure of Inconel 718 (IN718) during a 15-layer SLM process performed using a bi-directional 67° rotation scanning strategy and various SLM process parameters. The simulation results are found to be in good agreement with the experimental observations obtained in the present study and in the literature. In particular, a strong (001) texture is observed in the final component, which indicates that the grains with a preferred <001> orientation win the competitive epitaxy grain growth process. In addition, the size and shape of the IN718 grains are governed primarily by the cooling rate, where the cooling rate is determined in turn by the SLM parameters and the build height. Overall, the results show that the proposed framework provides an accurate approach for predicting the final microstructures of SLM components, and therefore, it can play an important role in optimizing the SLM processing parameters in such a way as to produce components with the desired mechanical properties.
Nonlinear ABAQUS Simulations for Notched Concrete Beams
Authors: Tawfik Ahmed Bahgat , Mahfouz Sameh Youssef , Taher Salah El-Din Fahmy
Published: December 2021

The numerical simulation of concrete fracture is difficult because of the brittle, inelastic-nonlinear nature of concrete. In this study, notched plain and reinforced concrete beams were investigated numerically to study their flexural response using different crack simulation techniques in ABAQUS. The flexural response was expressed by hardening and softening regime, flexural capacity, failure ductility, damage initiation and propagation, fracture energy, crack path, and crack mouth opening displacement. The employed techniques were the contour integral technique (CIT), the extended finite element method (XFEM), and the virtual crack closure technique (VCCT). A parametric study regarding the initial notch-to-depth ratio (ao/D), the shear span-to-depth ratio (S.S/D), and external post-tensioning (EPT) were investigated. It was found that both XFEM and VCCT produced better results, but XFEM had better flexural simulation. Contrarily, the CIT models failed to express the softening behavior and to capture the crack path. Furthermore, the flexural capacity was increased after reducing the (ao/D) and after decreasing the S.S/D. Additionally, using EPT increased the flexural capacity, showed the ductile flexural response, and reduced the flexural softening. Moreover, using reinforcement led to more ductile behavior, controlled damage propagation, and a dramatic increase in the flexural capacity. Furthermore, CIT showed reliable results for reinforced concrete beams, unlike plain concrete beams.
Investigation of the Size Effects on the Elongation of Ti-2.5Al-1.5Mn Foils with Digital Image Correlation Method
Authors: Wang Chunju , Zhang Weiwei , Ma Zhenwu
Published: December 2021

The increasing demand for parts with a large specific surface area such as fuel panels has put forward higher requirements for the plasticity of foils. However, the deformation characteristics of foils is hard to be illustrated in-depth due to their very short deformation process. In this paper, the digital image correlation method was applied to investigate the influence of size effect on the elongation of Ti-2.5Al-1.5Mn foils. The results showed that the elongation of Ti-2.5Al-1.5Mn foils increased with the decrease in the ratio of thickness-to-grain diameter (t/d value). Then, the macro deformation distribution of foils was analyzed, combined with their microstructure characteristics, and it was found that the increasing influence of individual grain heterogeneity leads to the earlier formation of a concentrated deformation zone, which changes the deformation mode of foils. The concentrated deformation increases with the decrease in t/d value, thus dominating the trend of the foil elongation. Furthermore, the homogeneous deformation and concentrated deformation can be divided into two different zones by a certain critical t/d value. These results provide a basis for understanding and further exploration of the deformation behavior of titanium foils.
Infrared Laser Speckle Projection-Based Multi-Sensor Collaborative Human Body Automatic Scanning System
Authors: Yang Xiao , Xi Juntong , Liu Jingyu , Chen Xiaobo
Published: December 2021

Human body scanning is an important means to build a digital 3D model of the human body, which is the basis for intelligent clothing production, human obesity analysis, and medical plastic surgery applications, etc. Comparing to commonly used optical scanning technologies such as laser scanning and fringe structured light, infrared laser speckle projection-based 3D scanning technology has the advantages of single-shot, simple control, and avoiding light stimulation to human eyes. In this paper, a multi-sensor collaborative digital human body scanning system based on near-infrared laser speckle projection is proposed, which occupies less than 2 m2 and has a scanning period of about 60 s. Additionally, the system calibration method and control scheme are proposed for the scanning system, and the serial-parallel computing strategy is developed based on the unified computing equipment architecture (CUDA), so as to realize the rapid calculation and automatic registration of local point cloud data. Finally, the effectiveness and time efficiency of the system are evaluated through anthropometric experiments.
A Computationally Efficient Model Predictive Control of Six-Phase Induction Machines Based on Deadbeat Control
Authors: Serra João , Jlassi Imed , Cardoso Antonio J. Marques
Published: December 2021

Model predictive current control (MPCC) has recently become a viable alternative for multiphase electric drives, because it easily exploits the inherent advantages of multi-phase machines. However, the prediction in MPCC requires a high number of voltage vectors (VVs), being therefore computationally demanding. In that regard, this paper proposes a computationally efficient MPCC of an asymmetrical six-phase induction machine drive (ASIMD) that reduces the number of VVs used for prediction. By using the characteristics of the deadbeat control (DB), the proposed method obtains a reference voltage vector (RVV), where its position will serve as a reference and integrates the MPCC scheme. Only 4 out of 13 predictions are needed to determine the best VV, dramatically reducing the algorithm computation. Experimental results for a six-phase case study compare the standard MPCC with the suggested method, confirming that deadbeat model predictive current control (DB-MPCC) shows that the execution time can be shortened by 48.8% and successfully improve the motor performance and efficiency.
Algorithm for Compression Design Allowable Determination of Composite Laminates with Initial Delaminations
Authors: Guo Jianchao , Zhang Yongbo , Chen Ke
Published: December 2021

With the increasing demands for detailed design of composite aircraft structures, the method of covering all damages with low design allowables cannot meet the current requirements for aircraft structure design. Herein, this paper proposes a novel algorithm for design allowable determination of composite laminates by combining the damage distribution with damage factor model of design allowable, so as to provide different structures with more accurate design allowables based on their initial damages. For the composite laminates with initial delaminations, a model describing the effect of delamination size and depth position on the compression design allowable is developed and the compression design allowable of different aircraft structures are individually determined by employing abundant initial delamination statistics. Compared with the design allowable offered by the single-point method, the design allowable based on the initial damage can be increased by at least 5% to 20%, greatly improving the economic benefits of the aircraft structures and providing an important support for the damage tolerance design of the composite structures.
Influence of Sampling Frequency Ratio on Mode Mixing Alleviation Performance: A Comparative Study of Four Noise-Assisted Empirical Mode Decomposition Algorithms
Authors: Zhao Yanqing , Adjallah Kondo H. , Sava Alexandre , Wang Zhouhang
Published: December 2021

Four noise-assisted empirical mode decomposition (EMD) algorithms, i.e., ensemble EMD (EEMD), complementary ensemble EMD (CEEMD), complete ensemble EMD with adaptive noise (CEEMDAN), and improved complete ensemble EMD with adaptive noise (ICEEMDAN), are noticeable improvements to EMD, aimed at alleviating mode mixing. However, the sampling frequency ratio (SFR), i.e., the ratio between the sampling frequency and the maximum signal frequency, may significantly impact their mode mixing alleviation performance. Aimed at this issue, we investigated and compared the influence of the SFR on the mode mixing alleviation performance of these four noise-assisted EMD algorithms. The results show that for a given signal, (1) SFR has an aperiodic influence on the mode mixing alleviation performance of four noise-assisted EMD algorithms, (2) a careful selection of SFRs can significantly improve the mode mixing alleviation performance and avoid decomposition instability, and (3) ICEEMDAN has the best mode mixing alleviation performance at the optimal SFR among the four noise-assisted EMD algorithms. The applications include, for instance, tool wear monitoring in machining as well as fault diagnosis and prognosis of complex systems that rely on signal decomposition to extract the components corresponding to specific behaviors.
Speed Control with Indirect Field Orientation for Low Power Three-Phase Induction Machine with Squirrel Cage Rotor
Authors: Gomes Robert R. , Pugliese Luiz F. , Silva Waner W. A. G. , Sousa Clodualdo V. , Rezende Guilherme M. , Rodor Fadul F.
Published: December 2021

Induction machines are widely used in the industry due to their many advantages compared to other industrial machines. This article presents the study and implementation of speed control applied to a Three-phase Induction Machine (MIT) of the squirrel cage type. The induction motor was modeled using the rotor flux in the synchronous reference to design Proportional-Integral (PI) type controllers for the current and velocity control loops. It is the objective of the article also to present in detail the development of converter hardware that comprises the stages of power, acquisition, and conditioning of engine signals. The system was simulated using computational tools and validated using a prototype designed, constructed, and commissioned.
Highly Effective Anti-Organic Fouling Performance of a Modified PVDF Membrane Using a Triple-Component Copolymer of P(Stx-co-MAAy)-g-fPEGz as the Additive
Authors: Zhou Xiaoji , Sun Yizhuo , Shen Shusu , Li Yan , Bai Renbi
Published: December 2021

In this study, a triple-component copolymer of P(Stx-co-MAAy)-g-fPEGz containing hydrophobic (styrene, St), hydrophilic (methacrylic acid, MAA), and oleophobic (perfluoroalkyl polyethylene glycol, fPEG) segments was synthesized and used as an additive polymer to prepare modified PVDF membrane for enhanced anti-fouling performance. Two compositions of St:MAA at 4:1 and 1:1 for the additive and two blending ratios of the additive:PVDF at 1:9 and 3:7 for the modified membranes were specifically examined. The results showed that the presence of the copolymer additive greatly affected the morphology and performance of the modified PVDF membranes. Especially, in a lower ratio of St to MAA (e.g., St:MAA at 1:1 versus 4:1), the additive polymer and therefore the modified PVDF membrane exhibited both better hydrophilic as well as oleophobic surface property. The prepared membrane can achieve a water contact angle at as low as 48.80° and display an underwater oil contact angle at as high as 160°. Adsorption experiments showed that BSA adsorption (in the concentration range of 0.8 to 2 g/L) on the modified PVDF membrane can be reduced by as much as 93%. From the filtration of BSA solution, HA solution, and oil/water emulsion, it was confirmed that the obtained membrane showed excellent resistance to these organic foulants that are often considered challenging in membrane water treatment. The performance displayed slow flux decay during filtration and high flux recovery after simple water cleaning. The developed membrane can therefore have a good potential to be used in such applications as water and wastewater treatment where protein and other organic pollutants (including oils) may cause severe fouling problems to conventional polymeric membranes.
Plasma Fibrinogen Independently Predicts Hypofibrinolysis in Severe COVID-19
Authors: Schrick Diana , Tőkés-Füzesi Margit , Réger Barbara , Molnár Tihamér
Published: December 2021

High rates of thrombosis are present in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Deeper insight into the prothrombotic state is essential to provide the best thromboprophylaxis care. Here, we aimed to explore associations among platelet indices, conventional hemostasis parameters, and viscoelastometry data. This pilot study included patients with severe COVID-19 (n = 21) and age-matched controls (n = 21). Each patient received 100 mg aspirin therapy at the time of blood sampling. Total platelet count, high immature platelet fraction (H-IPF), fibrinogen, D-dimer, Activated Partial Thromboplastin Time, von Willebrand factor antigen and von Willebrand factor ristocetin cofactor activity, plasminogen, and alpha2-antiplasmin were measured. To monitor the aspirin therapy, a platelet function test from hirudin anticoagulated whole blood was performed using the ASPI test by Multiplate analyser. High on-aspirin platelet reactivity (n = 8) was defined with an AUC > 40 cut-off value by ASPI tests. In addition, in vitro viscoelastometric tests were carried out using a ClotPro analyser in COVID-associated thromboembolic events (n = 8) (p = 0.071) nor the survival rate (p = 0.854) showed associations with high on-aspirin platelet reactivity status. The platelet count (p = 0.03), all subjects. COVID-19 patients presented with higher levels of inflammatory markers, compared with the controls, along with evidence of hypercoagulability by ClotPro. H-IPF (%) was significantly higher among non-survivors (n = 18) compared to survivors (p = 0.011), and a negative correlation (p = 0.002) was found between H-IPF and plasminogen level in the total population. The platelet count was significantly higher among patients with high on-aspirin platelet reactivity (p = 0.03). Neither the ECA-A10 (p = 0.008), and ECA-MCF (p = 0.016) were significantly higher, while the tPA-CFT (p < 0.001) was significantly lower among patients with high on-aspirin platelet reactivity. However, only fibrinogen proved to be an independent predictor of hypofibrinolysis in severe COVID-19 patients. In conclusion, a faster developing, more solid clot formation was observed in aspirin ‘non-responder’ COVID-19 patients. Therefore, an individually tailored thromboprophylaxis is needed to prevent thrombotic complications, particularly in the hypofibrinolytic cluster.
Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
Authors: Akasaka Rise , Ozawa Masashi , Nashimoto Yuji , Ino Kosuke , Shiku Hitoshi
Published: December 2021

We present a novel methodology based on ion conductance to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. The devices consisted of five channels, with the center channel filled with fibrin/collagen gel containing human umbilical vein endothelial cells (HUVECs). Fibroblasts were cultured in the other channels to improve the vascular network formation. To form vessel structures bridging the center channel, HUVEC monolayers were prepared on both side walls of the gel. During the culture, the HUVECs migrated from the monolayer and connected to the HUVECs in the gel, and vascular vessels formed, resulting in successful perfusion between the channels after culturing for 3–5 d. To evaluate perfusion without microscopic imaging, Ag/AgCl wires were inserted into the channels, and ion currents were obtained to measure the ion conductance between the channels separated by the HUVEC monolayers. As the HUVEC monolayers blocked the ion current flow, the ion currents were low before vessel formation. In contrast, ion currents increased after vessel formation because of creation of ion current paths. Thus, the observed ion currents were correlated with the perfusability of the vessels, indicating that they can be used as indicators of perfusion during vessel formation in microfluidic devices. The developed methodology will be used for drug screening using organs-on-a-chip containing vascular vessels.
Application of Laser Treatment in MOS-TFT Active Layer Prepared by Solution Method
Authors: Chen Nanhong , Ning Honglong , Liang Zhihao , Liu Xianzhe , Wang Xiaofeng , Yao Rihui , Zhong Jinyao , Fu Xiao , Qiu Tian , Peng Junbiao
Published: December 2021

The active layer of metal oxide semiconductor thin film transistor (MOS-TFT) prepared by solution method, with the advantages of being a low cost and simple preparation process, usually needs heat treatment to improve its performance. Laser treatment has the advantages of high energy, fast speed, less damage to the substrate and controllable treatment area, which is more suitable for flexible and large-scale roll-to-roll preparation than thermal treatment. This paper mainly introduces the basic principle of active layer thin films prepared by laser treatment solution, including laser photochemical cracking of metastable bonds, laser thermal effect, photoactivation effect and laser sintering of nanoparticles. In addition, the application of laser treatment in the regulation of MOS-TFT performance is also described, including the effects of laser energy density, treatment atmosphere, laser wavelength and other factors on the performance of active layer thin films and MOS-TFT devices. Finally, the problems and future development trends of laser treatment technology in the application of metal oxide semiconductor thin films prepared by solution method and MOS-TFT are summarized.
A 3D Microfluidic ELISA for the Detection of Severe Dengue: Sensitivity Improvement and Vroman Effect Amelioration by EDC–NHS Surface Modification
Authors: Maeno Hinata , Wong Pooi-Fong , AbuBakar Sazaly , Yang Ming , Sam Sing-Sin , Jamil-Abd Juraina , Shunmugarajoo Anusha , Mustafa Mahiran , Said Rosaida Md , Mageswaren Eashwary , Azmel Azureen , Mat Jelani Anilawati
Published: December 2021

Serum is commonly used as a specimen in immunoassays but the presence of heterophilic antibodies can potentially interfere with the test results. Previously, we have developed a microfluidic device called: 3D Stack for enzyme-linked immunosorbent assay (ELISA). However, its evaluation was limited to detection from a single protein solution. Here, we investigated the sensitivity of the 3D Stack in detecting a severe dengue biomarker—soluble CD163 (sCD163)—within the serum matrix. To determine potential interactions with serum matrix, a spike-and-recovery assay was performed, using 3D Stacks with and without surface modification by an EDC–NHS (N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) coupling. Without surface modification, a reduced analyte recovery in proportion to serum concentration was observed because of the Vroman effect, which resulted in competitive displacement of coated capture antibodies by serum proteins with stronger binding affinities. However, EDC–NHS coupling prevented antibody desorption and improved the sensitivity. Subsequent comparison of sCD163 detection using a 3D Stack with EDC–NHS coupling and conventional ELISA in dengue patients’ sera revealed a high correlation (R = 0.9298, p < 0.0001) between the two detection platforms. Bland–Altman analysis further revealed insignificant systematic error between the mean differences of the two methods. These data suggest the potentials of the 3D Stack for further development as a detection platform.
Topological Nanophotonic Wavelength Router Based on Topology Optimization
Authors: Yuan Hongyi , Liu Zhouhui , Wei Maoliang , Lin Hongtao , Hu Xiaoyong , Lu Cuicui
Published: December 2021

The topological nanophotonic wavelength router, which can steer light with different wavelength signals into different topological channels, plays a key role in optical information processing. However, no effective method has been found to realize such a topological nanophotonic device. Here, an on-chip topological nanophotonic wavelength router working in an optical telecom band is designed based on a topology optimization algorithm and experimentally demonstrated. Valley photonic crystal is used to provide a topological state in the optical telecom band. The measured topological wavelength router has narrow signal peaks and is easy for integration. This work offers an efficient scheme for the realization of topological devices and lays a foundation for the future application of topological photonics.
Coordinating Carbon Metabolism and Cell Cycle of Chlamydomonasreinhardtii with Light Strategies under Nitrogen Recovery
Authors: Ren Yuanyuan , Sun Han , Deng Jinquan , Zhang Yue , Li Yuelian , Huang Junchao , Chen Feng
Published: December 2021

Nutrient supplementation is common in microalgae cultivation to enhance the accumulation of biomass and biofunctional products, while the recovery mechanism from nutrient starvation is less investigated. In this study, the influence of remodeled carbon metabolism on cell cycle progression was explored by using different light wavelengths under N-repletion and N-recovery. The results suggested that blue light enhanced cell enlargement and red light promoted cell division under N-repletion. On the contrary, blue light promoted cell division by stimulating cell cycle progression under N-recovery. This interesting phenomenon was ascribed to different carbon metabolisms under N-repletion and N-recovery. Blue light promoted the recovery of photosystem II and redirected carbon skeletons into proteins under N-recovery, which potentially accelerated cell recovery and cell cycle progression. Although red light also facilitated the recovery of photosystem II, it mitigated the degradation of polysaccharide and then arrested almost all the cells in the G1 phase. By converting light wavelengths at the 12 h of N-recovery with blue light, red and white lights were proved to increase biomass concentration better than continuous blue light. These results revealed different mechanisms of cell metabolism of Chlamydomonas reinhardtii during N-recovery and could be applied to enhance cell vitality of microalgae from nutrient starvation and boost biomass production.
In Vitro Evaluation of the Effect of Oral Probiotic Weissella cibaria on the Formation of Multi-Species Oral Biofilms on Dental Implant Surfaces
Authors: Kang Mi-Sun , Park Geun-Yeong
Published: December 2021

Oral probiotics are beneficial bacteria that can help prevent periodontal disease. However, little is known about the effects of oral probiotics on the formation of implant biofilms. This study aimed to evaluate the effects of oral probiotics Weissella cibaria CMU and CMS1 in an in vitro complex biofilm model on titanium implant surfaces. First, it was identified through colony biofilm assay that W. cibaria CMU and CMS1 inhibit the formation of multi-species biofilms formed by eight types of bacteria. Two types of saliva-coated titanium discs inoculated with early (Streptococcus gordonii, Streptococcus oralis, Streptococcus sanguinis, Actinomyces naeslundii, and Veillonella parvula), secondary (Fusobacterium nucleatum and Prevotella intermedia), and late (Porphyromonas gingivalis) colonizers were treated with the oral probiotics and then incubated anaerobically for three days. The effects of oral probiotics on titanium disc biofilm formation were analyzed using culture methods, quantitative polymerase chain reaction (qPCR), and microscopic analysis. Both probiotics significantly inhibited the formation of biofilm, and all eight bacterial species were significantly reduced. The effectiveness of both probiotic strains was confirmed by all the methods used. Oral probiotics may have dramatically reduced the biofilm formation of secondary colonizers that act as bridges, thus inhibiting biofilm formation on the titanium surface. Our results suggest that the probiotic W. cibaria offers new possibilities for the prevention of peri-implant mucositis.
Emerging Trends of Multidrug-Resistant (MDR) and Extensively Drug-Resistant (XDR) Salmonella Typhi in a Tertiary Care Hospital of Lahore, Pakistan
Authors: Zakir Muhammad , Khan Maryam , Umar Muhammad Ihtisham , Murtaza Ghulam , Ashraf Muhammad , Shamim Saba
Published: December 2021

Salmonella Typhi is a Gram-negative pathogen that causes typhoid fever in humans. The use of antibiotics to treat typhoid has considerably mitigated its fatality risk, but rising multidrug-resistant (MDR) and extensively drug-resistant (XDR) resistance in Pakistan threatens effective treatment. This study determined the prevalence of MDR and XDR S. Typhi at a local hospital in Lahore. Blood samples (n = 3000) were obtained and processed for bacterial identification. Antibiotic susceptibility test was performed using VITEK® 2 Compound 30 System. Statistical data analysis was performed using a Mann–Whitney U and Kruskal–Wallis H test, respectively. The results revealed 600 positive cultures, of which the majority were found to be XDR S. Typhi (46.1%) and MDR S. Typhi (24.5%) strains. The disease burden of resistant Salmonella strains was greater in males (60.67%) than females (39.33%), with the most affected age group being 0–10 years old (70.4 %). In both the outpatient department (OPD) and general ward, the prevalence of XDR S. Typhi cases was found to be alarmingly high (48.24%), followed by MDR S. Typhi (25.04 %). The results of the statistical analysis demonstrated that the incidence of resistance in MDR and XDR S. Typhi strains was not affected by the age as well as the gender of patients (p > 0.05). The occurrence of resistant strains against four tested antibiotics (azithromycin, ciprofloxacin, imipenem, and meropenem) was found to be similar in different wards and among hospitalized and OPD patients (p > 0.05). Maximum resistance was observed against chloramphenicol and ampicillin in the OPD and pediatric ward. Piperacillin/Tazobactam was observed to be the most effective antibiotic, followed by co-amoxiclav (p < 0.001). This study is effective in validating the existence of MDR and XDR S. Typhi in Lahore, where stringent methods should be applied for controlling its spread.
The Odor Release Regularity of Livestock and Poultry Manure and the Screening of Deodorizing Strains
Authors: Ma Haixia , Li Feier , Niyitanga Evode , Chai Xicun , Wang Shipeng , Liu Yutao
Published: December 2021

Human living environments and health are seriously affected by the odor produced from fermentation of livestock and poultry manure. In order to reduce the odor pollution caused by livestock and poultry manure, efficient strains were screened and two methods were tried in this study. The orthogonal test design was used to analyze the gas produced by pig manure under different conditions of temperature, time, wheat straw doping amount and calcium carbonate doping amount. Then, according to ammonia, hydrogen sulfide and comprehensive odor removal effects, the high efficiency of deodorizing strains were screened. The results showed that pig manure produced the least odor when the temperature was 20 °C, added 0% calcium carbonate, 20% wheat straw and waited for 48 h. Three strains were screened to inhibit the odor production of pig manure: Paracoccus denitrificans, Bacillus licheniformis and Saccharomyces cerevisiae, showed that their highest removal rate of ammonia and hydrogen sulfide gas could reach 96.58% and 99.74% among them; while for three strains of end-control pig manure stench: Pichia kudriavzevii, P. denitrificans and Bacillus subtilis, the highest removal rate of ammonia and hydrogen sulfide gas reached 85.91% and 90.80% among them. This research provides bacteria resources as the high-efficiency deodorizing function for the source suppression and the end treatment of the odor gas of pig manure, which has high application value for the control of odor pollution.
VIS-NIR/SWIR Spectral Properties of H2O Ice Depending on Particle Size and Surface Temperature
Authors: Stephan Katrin , Ciarniello Mauro , Poch Olivier , Schmitt Bernard , Haack David , Raponi Andrea
Published: December 2021

Laboratory measurements were performed to study the spectral signature of H2O ice between 0.4 and 4.2 µm depending on varying temperatures between 70 and 220 K. Spectral parameters of samples with particle sizes up to ~1360 µm, particle size mixtures, and different particle shapes were analyzed. The band depth (BD) of the major H2O-ice absorptions at 1.04, 1.25, 1.5, and 2 µm offers an excellent indicator for varying particle sizes in pure H2O ice. The spectral changes due to temperature rather, but not exclusively, affect the H2O-ice absorptions located at 1.31, 1.57, and 1.65 µm and the Fresnel reflection peaks at 3.1 and 3.2 µm, which strongly weaken with increasing temperature. As the BDs of the H2O-ice absorptions at 1.31, 1.57, and 1.65 µm increase, the band centers (BCs) of the H2O-ice absorptions at 1.25 and 1.5 µm slightly shift to shorter wavelengths. However, the BCs of the strong H2O-ice absorptions can also be affected by saturation in the case of large particles. The collected spectra provide a useful spectral library for future investigations of icy satellites such as Ganymede and Callisto, the major targets of ESA’s JUICE mission.
Bio-Organic Mineral Fertilizer for Sustainable Agriculture: Current Trends and Future Perspectives
Authors: Syed Shameer , Wang Xingxing , Prasad Tollamadugu N.V.K.V. , Lian Bin
Published: December 2021

Chemical (synthetic) fertilizers used indiscriminately for improved production pose a major threat to long-term soil fertility, the soil environment, and its components. The soil microbial community, however, plays a major and important role in fostering soil health and plant growth. While the use of synthetic fertilizers has a profound impact on plant growth, it also significantly alters the makeup of the microbial community towards a detrimental low, especially N and P fertilizers. Sustainable farming practices can reduce the depletion of natural resources and maintain both productivity and soil fertility. The use of minerals that contain fertilizer nutrients in their native state is a very promising approach to reducing emissions associated with the processing chemical industries. Organic material from natural sources (food waste, manure from livestock, agricultural biomass, etc.) acts as a source of microbial culture and encourages the release of nutrients into the soil during mineral weathering. The combination of nutrient-bearing minerals and their biological weathering agents together with organic matter has the potential to remediate, restore, and sustain depleted agricultural soils. Therefore, in this review, we emphasize the significance of sustaining agricultural productivity and microbial diversity in the rhizosphere, the two vital aspects of modern agricultural systems, through bio-organic mineral fertilizers.
Strain Localization at Constant Strain Rate and Changing Stress Conditions: Implications for Plate Boundary Processes in the Upper Mantle
Authors: Newman Julie , Chatzaras Vasileios , Tikoff Basil , Wijbrans Jan R. , Lamb William M. , Drury Martyn R.
Published: December 2021

We present results from a natural deformed shear zone in the Turon de Técouère massif of the French Pyrenees that directly addresses the processes involved in strain localization, a topic that has been investigated for the last 40 years by structural geologists. Paleopiezometry indicates that differential stresses are variable both spatially across the zone, and temporally during exhumation. We have, however, also calculated strain rate, which remains constant despite changes in stress. This result appears to be at odds with recent experimental deformation on monophase (olivine) rocks, which indicate that strain localization occurs dominantly as a result of constant stress. We hypothesize that in the Turon de Técouère massif—and many natural shear zones—strain localization occurs as a result of reactions, which decrease the grain size and promote the activation of grain size sensitive deformation mechanisms. From a tectonics perspective, this study indicates that the deformation rate in a particular plate boundary is relatively uniform. Stress, however, varies to accommodate this deformation. This viewpoint is consistent with deformation at a plate boundary, but it is not the typical way in which we interpret strain localization.
Diagenesis and the Conditions of Deposition of the Middle Jurassic Siderite Rocks from the Northern Margin of the Holy Cross Mountains (Poland)
Authors: Kozłowska Aleksandra , Feldman-Olszewska Anna , Kuberska Marta , Maliszewska Anna
Published: December 2021

The aim of the present study is to reconstruct sedimentary conditions of Middle Jurassic rocks that contain siderites to identify the mineral composition of the inserbeds and to recognize the origin of the siderite. Thin inserbeds of siderite rocks occur most frequently within Bajocian siliciclastic deposits and, more rarely, Aalenian and Bathonian. The research material comes from 11 boreholes located in the north and northeastern margins of the Holy Cross Mountains. The research methods included sedimentological analyses, and studies in polarizing and scanning electron microscopes, staining of carbonates, cathodoluminescence, X-ray structural analysis, and stable carbon and oxygen isotopic determinations were used. Middle Jurassic sideritic rocks are most often represented by clayey siderites, which also include muddy and sandy varieties and siderite sandstones. There are also local occurrences of coquinas, claystones, mudstones, and siderite conglomerates. The main component of sideritic rocks is sideroplesite. Berthierine, pistomesite, calcite, and ankerite are important components, too. The action of diagenetic processes of cementation, compaction, replacement, and alteration within the Middle Jurassic deposits was most intense during the eo- and mesodiagenesis. The sedimentological analysis showed that most of the studied siderites were formed in a low-oxygenated marine environment, mainly in the transition zone between the normal and storm wave bases and in the lower and middle shoreface zones. The results of the petrographic, mineralogical, and geochemical studies indicated the origin of the sideritic rocks mainly in the marine environment, with the participation of meteoric water. There were slight differences in the chemical composition of sideroplesite depending on the environment it crystallized in. There was no correlation between the values of the carbon isotope determinations in the sideroplesite and the environmental conditions of its crystallization. Slight differences were visible in the case of the average values of δ18O in the sideroplesite.
Iron Isotope Composition of Adakitic Rocks: The Shangcheng Pluton, Western Dabie Orogen, Central China
Authors: Deng Chenglai , Hu Changqing , Li Ming , Li Wu
Published: December 2021

There has been little research on the metal isotopic composition of adakitic rock. The main objective of our investigation was to obtain more knowledge on the iron isotopic composition of adakitic rocks and provide new evidence for the genesis of Shangcheng pluton from an iron isotope perspective. The Dabie orogen is divided into eastern and western areas by the Shangcheng-Macheng fault, and the Shangcheng pluton is located in the western Dabie orogen area. The iron isotopic composition of these rocks ranges from 0.08‰ to 0.20‰ (2SD, n = 3). The δ56Fe values of two rocks from the SGD (Sigudun) unit are relatively low (0.11 ± 0.03‰ and 0.08 ± 0.04‰), while the δ56Fe values of the other samples are basically consistent (0.18–0.2‰). Evidence from elemental geochemical characteristics and petrogenesis defines the Shangcheng pluton as adakitic rocks. Our investigation on the elemental and isotopic compositions hints that the enrichment of heavy iron isotopes cannot be explained by weathering/alteration and fluid exsolution. Fractional crystallization of magnetite may account for the enrichment of light iron isotopes in two rocks from the SGD unit, while the fractional iron isotope trend in the other five samples can be explained by Δ56Fecrystal-melt = ~0.035‰. Two investigated rocks from SGD units may have been derived from the partial melting of amphibolite, while the other five samples may have been derived from the partial melting of eclogite containing 10–15% garnet.
The Influence of 5′,8-Cyclo-2′-deoxypurines on the Mitochondrial Repair of Clustered DNA Damage in Xrs5 Cells: The Preliminary Study
Authors: Boguszewska Karolina , Kaźmierczak-Barańska Julia , Karwowski Bolesław T.
Published: December 2021

The 5′,8-cyclo-2′-deoxypurines (cdPus) affect the DNA structure. When these bulky structures are a part of clustered DNA lesions (CDL), they affect the repair of the other lesions within the cluster. Mitochondria are crucial for cell survival and have their own genome, hence, are highly interesting in the context of CDL repair. However, no studies are exploring this topic. Here, the initial stages of mitochondrial base excision repair (mtBER) were considered—the strand incision and elongation. The repair of a single lesion (apurinic site (AP site)) accompanying the cdPu within the double-stranded CDL has been investigated for the first time. The type of cdPu, its diastereomeric form, and the interlesion distance were taken into consideration. For these studies, the established experimental model of short oligonucleotides (containing AP sites located ≤7 base pairs to the cdPu in both directions) and mitochondrial extracts of the xrs5 cells were used. The obtained results have shown that the presence of cdPus influenced the processing of an AP site within the CDL. Levels of strand incision and elongation were higher for oligos containing RcdA and ScdG than for those with ScdA and RcdG. Investigated stages of mtBER were more efficient for DNA containing AP sites located on 5′-end side of cdPu than on its 3′-end side. In conclusion, the presence of cdPus in mtDNA structure may affect mtBER (processing the second mutagenic lesion within the CDL). As impaired repair processes may lead to serious biological consequences, further studies concerning the mitochondrial repair of CDL are highly demanded.
Solvothermal Synthesis of a Novel Calcium Metal-Organic Framework: High Temperature and Electrochemical Behaviour
Authors: Main Russell M. , Cordes David B. , Desai Aamod V. , Slawin Alexandra M. Z. , Wheatley Paul , Armstrong A. Robert , Morris Russell E.
Published: December 2021

The rapid growth in the field of metal-organic frameworks (MOFs) over recent years has highlighted their high potential in a variety of applications. For biological and environmental applications MOFs with low toxicity are vitally important to avoid any harmful effects. For this reason, Ca-based MOFs are highly desirable owing to their low cost and high biocompatibility. Useful Ca MOFs are still rare owing to the ionic character and large size of the Ca2+ ion tending to produce dense phases. Presented here is a novel Ca-based MOF containing 2,3-dihyrdoxyterephthalate (2,3-dhtp) linkers Ca(2,3-dhtp)(H2O) (SIMOF-4). The material undergoes a phase transformation on heating, which can be followed by variable temperature powder X-ray diffraction. The structure of the high temperature form was obtained using single-crystal X-ray diffraction. The electrochemical properties of SIMOF-4 were also investigated for use in a Na ion battery.
Synthesis and Characterization of the Ethylene-Carbonate-Linked L-Valine Derivatives of 4,4-Dimethylcurcumin with Potential Anticancer Activities
Authors: Lee Der-Yen , Lin Hui-Yi , Ramasamy Manickavasakam , Kuo Sheng-Chu , Lee Pei-Chih , Hsieh Min-Tsang
Published: December 2021

Natural phenolic products from herbal medicines and dietary plants constitute the main source of lead compounds for the development of the new drug. 4,4-Dimethylcurcumin (DMCU) is a synthetic curcumin derivative and exhibits anticancer activities against breast, colon, lung, and liver cancers. However, further development of DMCU is limited by unfavorable compound properties such as very low aqueous solubility and moderate stability. To increase its solubility, we installed either or both of the ethylene-carbonate-linked L-valine side chains to DMCU phenolic groups and produced targeted 1-trifluoroacetic acid (1-TFA) and 2-trifluoroacetic acid (2-TFA) derivatives. The terminus L-valine of ethylene-carbonate-linked side chain is known to be a L-type amino acid transporter 1 (LAT1) recognition element and therefore, these two derivatives were expected to readily enter into LAT1-expressing cancer cells. In practice, 1-TFA or 2-TFA were synthesized from DMCU in four steps with 34–48% overall yield. Based on the corresponding LC-MS analysis, water solubility of DMCU, 1-TFA, and 2-TFA at room temperature (25 ± 1 °C) were 0.018, 249.7, and 375.8 mg/mL, respectively, indicating >10,000-fold higher solubility of 1-TFA and 2-TFA than DMCU. Importantly, anti-proliferative assay demonstrated that 2-TFA is a potent anti-cancer agent against LAT1-expressing lung cancer cells NCI-H460, NCI-H358, and A549 cells due to its high intracellular uptake compared to DMCU and 1-TFA. In this study, we logically designed and synthesized the targeted compounds, established the LC-MS analytical methods for evaluations of drug solubility and intracellular uptake levels, and showed improved solubility and anti-cancer activities of 2-TFA. Our results provide a strategical direction for the future development of curcuminoid-like phenolic compounds.
Theoretical Investigation of the Fusion Process of Mono-Cages to Tri-Cages with CH4/C2H6 Guest Molecules in sI Hydrates
Authors: Wei Shuxian , Liu Siyuan , Cao Shoufu , Zhou Sainan , Chen Yong , Wang Zhaojie , Lu Xiaoqing
Published: December 2021

Owing to a stable and porous cage structure, natural gas hydrates can store abundant methane and serve as a potentially natural gas resource. However, the microscopic mechanism of how hydrate crystalline grows has not been fully explored, especially for the structure containing different guest molecules. Hence, we adopt density functional theory (DFT) to investigate the fusion process of structure I hydrates with CH4/C2H6 guest molecules from mono-cages to triple-cages. We find that the volume of guest molecules affects the stabilities of large (51262, L) and small (512, s) cages, which are prone to capture C2H6 and CH4, respectively. Mixed double cages (small cage and large cage) with the mixed guest molecules have the highest stability and fusion energy. The triangular triple cages exhibit superior stability because of the three shared faces, and the triangular mixed triple cages (large-small-large) structure with the mixed guest molecules shows the highest stability and fusion energy in the triple-cage fusion process. These results can provide theoretical insights into the growth mechanism of hydrates with other mono/mixed guest molecules for further development and application of these substances.