bluuebunny/crossref_metadata_2025 huggingface dataset:
Who Gets to Breastfeed? A Narrative Ecological Analysis of Women's Infant Feeding Experiences in the UK
Authors: Thomson Gill , Ingram Jenny , Clarke Joanne , Johnson Debbie , Jolly Kate
Published: July 2022

The early post-natal period is a critical period in women's infant feeding journeys, often marked by high levels of unintended breastfeeding cessation. Previous research has argued that infant feeding should be perceived within a complex system whereby factors operating at different ecological levels (i.e., individual, social/community networks, cultural/institutional) interact to affect individual behaviors. However, currently, more work needs to be done to implement an ecological approach in breastfeeding programs. We adopted a complex-systems lens approach to explore how multi-level factors—individual, mother-infant dyad, health service, family and social networks, and wider community infrastructure—interacted with women's motivations and experiences of breastfeeding. We undertook a secondary analysis of 24 women's interviews; all the women had a strong antenatal intention to breastfeed and were expecting their first baby. The interviews were collected during the UK-based Assets-based feeding help Before and After birth (ABA) feasibility trial when their infant was aged between 4 and 21 weeks. Categorical content analysis was used to explore the interrelationships between key factors and to identify different infant feeding typologies. Two different typologies emerged: “disappointed” (n = 7) and “by hook or by crook” (n = 17). “Disappointed” women had stopped breastfeeding early; women classified as “by hook or by crook” continued breastfeeding despite facing challenges. Sociodemographic, social, and service level differences between the typologies were noted. “Disappointed” women were more likely to be younger, White-British, to have considered mixed-feeding antenatally and experienced negative breastfeeding support from healthcare professionals and personal networks. Infants of “disappointed” women were more likely to have received unexpected “top-ups” and to be perceived as having infant feeding difficulties. Women classified as “by hook or by crook” were just as likely as “disappointed” women to experience birth-related complications, but demonstrated more proactive help-seeking behaviors, had positive experiences of personal/professional support and accessed wider support. While further research is needed to consolidate and/or refute the typologies, the ecological approach shifts the focus away from mothers' decisions to consider the multi-level factors that need to be in place to enable women to breastfeed successfully. Further work to encourage help-seeking behaviors and toward improving facilities, support, and services is needed.
Phosphorus Stock Depletion and Soil C:N:P Stoichiometry Under Annual Crop Rotations and Grassland Management Systems Over 13 Years
Authors: Somavilla André , Caner Laurent , da Silva Isley Cristiellem Bicalho , Rheinheimer Danilo dos Santos , Chabbi Abad
Published: July 2022

Phosphorus (P) nutrition is essential to both plant yield and soil organic matter (SOM) input. However, continuous extraction of P by plants and biomass harvesting can lead to soil P stock depletion, a reduction in crop yields and ultimately a reduction in organic matter input to the soil. In this work, we analysed P, C and N stock trends in the 0-30 cm topsoil layer cultivated with permanent cropland (CC) and mowed permanent grassland (GG) for 13 years. In addition, we characterized the changes in P organic forms by using 31P-NMR. The results showed that the amount of P exported within 13 years was 10% greater in GG than in CC (295 and 268 kg ha-1, respectively). The total P stocks decreased under both the CC and GG management systems (0.30 and 0.25 Mg ha-1, respectively). This depletion was mainly observed in total Pi forms, which recorded reductions of 0.75 and 0.29 Mg ha-1 in GG and CC, respectively. The total Po stock increased by 42.6% in GG; these results were consistent with the increase in C and N stocks in GG (2.6 and 0.19 Mg ha-1, respectively) and their reduction in CC (-4.2 and -0.38 Mg ha-1, respectively). Although P depletion mainly affected the P pool presenting the highest lability (labile P), this depletion did not have a negative effect on plant yield after 13 years due to the buffering capacity of P pools presenting less lability (moderately labile P). Mowing permanent grasslands led to a change in the pool of labile P from inorganic to organic forms and an increase in soil C and N stocks. Based on the 31P-NMR technique, permanent grasslands significantly reduced α-glycerophosphate and increased myo-IHP and adenosine monophosphate more than the permanent cropland. Although there was no significant decrease in productivity, the depletion of available P should be monitored over time, especially in mown permanent grassland crops, to prevent potential nutrient stress.
Time-frequency analysis of HD 174936 and HD 174966 using the synchrosqueezed wavelet transform
Authors: Ramón-Ballesta A. , Pascual-Granado J. , Garrido R.
Published: July 2022

δ Scuti stars are intermediate-mass pulsators located in the classical Cepheid instability strip with spectral types ranging from A to F. In the last years, some authors found an amplitude and frequency modulation in some of these stars. In our work we have chosen the continuous wavelet transform as the more appropriate tool to perform a time-frequency analysis of the light curves of HD 174936 and HD 174966. We have chosen this tool because, unlike the short time Fourier transform, that uses a fixed windows size, the continuous wavelet transform uses short windows at high frequencies and long windows at low frequencies, making it like an adaptive analysis tool. In order to improve the resolution of the frequencies obtained by the continuous wavelet transform, we have also used the synchrosqueeze algorithm to “focus” the result. Then, we are capable to obtain a measure of the instantaneous frequencies of the signal in a wide range of frequencies. To check our results, we have compared them with the synchrosqueezed continuous wavelet transform of a synthetic signal generated with the frequencies previously obtained by a classical approach. Our results show that some frequencies are stable, at least within the observation run time, although some others show some kind of variation. This is the first time that such variations in the oscillation frequencies of HD 174936 and HD 174966 are reported.
A participatory assessment of nitrified urine fertilizer use in Swayimane, South Africa: Crop production potential, farmer attitudes and smallholder challenges
Authors: Wilde Benjamin C. , Lieberherr Eva , Pereira Engil , Odindo Alfred , Six Johan
Published: July 2022

Long-term nutrient mining of soil hampers agricultural production across Africa. However, emerging sanitation technologies afford a hygienically safe and ecologically sustainable solution to this development challenge by providing fertilizers derived from human excreta that could facilitate a socio-technical transition toward a more sustainable food system. To evaluate one such technology, nitrified urine fertilizer (NUF), we conducted participatory action research to assess the potential, from both a biophysical and social perspective, of NUF to serve as a soil fertilizer to support smallholder agricultural production in Swayimane, South Africa. To achieve this objective, we formed a stakeholder group comprised of a cooperative of smallholder farmers, a local NGO (Zimele), and researchers from ETH Zurich and the University of Kwazulu-Natal. Over the course of two growing seasons (2016 and 2017) this stakeholder group assessed the potential of NUF to support smallholder vegetable production (i.e., cabbage). First, we adopted a randomized complete block design incorporating five treatments in season 1 (unfertilized control, nitrified urine, nitrified urine+bone meal, urea, and urea+diammonium phosphate (DAP) and six treatments (unfertilized control, urea, urea+DAP, DAP, nitrified urine, and nitrified urine+DAP) in season 2 to assess cabbage yield and leaf nutrient concentration (sodium, phosphorus, potassium, carbon, nitrogen). Although we observed large variability in yields, the urine-based treatments were as effective as any of the chemical fertilizers. Second, beyond the biophysical analysis, we elicited the challenges and opportunities of the smallholder farmers in our stakeholder group, as well as their attitudes toward the use of NUF as a fertilizer. Through this qualitative work, farmers indicated that their attitudes about the use of NUF as a fertilizer improved and that they would be willing to incorporate this product into their production practices if it was available at scale. Thus, we demonstrate the potential of participatory action research to co-produce knowledge and awareness around an innovative technology. In so doing, we provide evidence that this approach can support a change toward nutrient recycling-based agriculture.
Rice Yield and Nitrogen Use Efficiency With System of Rice Intensification and Conventional Management Practices in Mkindo Irrigation Scheme, Tanzania
Authors: Mboyerwa Primitiva Andrea , Kibret Kibebew , Mtakwa Peter , Aschalew Abebe
Published: July 2022

Aim

This study investigated the impacts of system of rice intensification (SRI) and conventional management practice (CP) on rice growth, grain yield, and nitrogen use efficiency by nitrogen application.

Methods

Field experiments were conducted in wet and dry seasons; each season, the experiment was set in a split-plot randomized complete block design in triplicate with crop management practices in main plots and nitrogen levels in subplots.

Results

The average grain yield by SRI was 7.1 and 6.7 t ha−1, while by CP it was 6.1 and 4.4 t ha−1in the wet and dry seasons, respectively. The grain yield of the SRI practice was significantly (p < 0.05) greater than that of the conventional practice (CP) at all levels of nitrogen application. The average yield under the treatment interaction of SRI and nitrogen levels were increased by 13.1% in the wet season and 35.8 % in the dry season. Roots of SRI plants had significantly (p < 0.05) greater fresh weight, length, and volume as indicated by increased root dry weight per hill. SRI improved crop growth, effective tillers, filled grains per panicle, grain filling rate, panicle weight, spikelet per panicle, straw yield, and 1,000-grain weight. Nitrogen application rate had a significant effect (p < 0.05) on agronomic nitrogen use efficiency (ANUE). As the N application rate was increased beyond 90 kg N ha−1, the ANUE and partial factor productivity (PFP) under both SRI and CP were significantly decreased in both seasons.

Conclusion

Overall, the SRI production system with 60 kg N ha−1 improved rice growth, yield, and nitrogen use efficiency compared to the CP.
Modified subxiphoid approach for surgical resection of a retrosternal goiter
Authors: Wang Renfeng , Li Jianfeng , Jiang Jiahao , Ding Jianyong , Yang Minghui , Wang Shuai , Lin Miao
Published: July 2022

Backgrounds

Unilateral Video-Assisted Thorascopic Surgery (VATS) is a traditional minimally invasive transthoracic approach for the surgical resection of a subxiphoid goiter. Recently, the subxiphoid approach was recommended for an anterior mediastinal mass. This study aims to investigate the feasibility and efficacy of a modified subxiphoid VATS for the resection of a retrosternal goiter as an alternative transthoracic approach.

Methods

We retrospectively collected all patients who underwent subxiphoid VATS for the resection of a retrosternal goiter from June 2017 to June 2021 in the Zhongshan Hospital or the Zhongshan Hospital Xiamen branch. Ten patients were found. Patient characteristics, perioperative data, and surgical information were collected and further analyzed.

Results

In our study, all 10 patients underwent a thoracoscopic subxiphoid resection of a retrosternal goiter. The mean age was 49.4 years, and all were female. The majority of patients (70%) were asymptomatic. All patients were assessed by CT imaging before surgery. The mean postoperative hospital stay was 4.9 days. The drainage tube was removed 3 days after operation, and the average drainage volume was 73.1 ml. Postoperative pain was mild, with an average pain grade of 2.4 (measured on a scale from 0 to 10, with lower scores indicating less pain). There were no conversions or perioperative complications in these 10 patients.

Conclusions

Most retrosternal goiters can be completely resected through the modified subxiphoid approach after an adequate preoperative evaluation and careful intraoperative management. This thoracoscopic subxiphoid approach is feasible and safe for retrosternal goiter resection.
Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries)
Authors: Du Xiaolong , He Xiaoyun , Liu Qingqing , Liu Qiuyue , Di Ran , Chu Mingxing
Published: July 2022

In seasonal estrus, it is well known that melatonin-regulated biorhythm plays a key role. Some studies indicate that the adrenal gland plays an important role in reproduction in mammals, but the molecular mechanism is not clear. This study used an artificially controlled light photoperiod model, combined with RNA-seq technology and bioinformatics analysis, to analyze the messenger RNA (mRNA) and microRNA (miRNA) of ewe (Sunite) adrenal glands under different photoperiod treatments. After identification, the key candidate genes GRHL2, CENPF, FGF16 and SLC25A30 that photoperiod affects reproduction were confirmed. The miRNAs (oar-miR-544-3p, oar-miR-411b-5p, oar-miR-376e-3p, oar-miR-376d, oar-miR-376b-3p, oar-miR-376a-3p) were specifically expressed in the adrenal gland. The candidate mRNA-miRNA pairs (e.g., SLC25A30 coagulated by novel miRNA554, novel miRNA555 and novel miRNA559) may affect seasonal estrus. In summary, we constructed relation network of the mRNAs and miRNAs of sheep adrenal glands using RNA sequencing and bioinformatics analysis, thereby, providing a valuable genetic variation resource for sheep genome research, which will contribute to the study of complex traits in sheep.
Epidemiological Survey and Confirmation of Autochthonous Cases of Bovine Fasciolosis in the Serrana Mesoregion of Santa Catarina, Brazil
Authors: Américo Larissa , Padilha Mayckon Antonio Cardoso , Arruda Paula Maciel , Drescher Guilherme , de Moura Anderson Barbosa , Chryssafidis Andreas Lazaros
Published: July 2022

Fasciola hepatica is reported as a trematode of great importance, causing fasciolosis, one of the most neglected zoonotic parasitic diseases in the world. In Brazil, potential losses of around US$ 210 million per year were calculated, with bovine fasciolosis alone. The State of Santa Catarina is located in the South Region of Brazil, with a herd of more than 4 million ruminants. The Serrana Mesoregion, historically free of fasciolosis, is composed of 30 municipalities, which have a consolidated agricultural tradition, mainly in extensive ruminant livestock. The objective of the present work was to investigate the occurrence and distribution of autochthonous cases of bovine fasciolosis in the region. For this, different analyzes were carried out in rural properties from eight municipalities, and in three slaughterhouses under the State Inspection Service (SIE), which received animals from all the Serrana Mesoregion. The rural properties were randomly selected, and up to 20 fecal samples were collected from random cattle older than 1 year, for analysis by coproparasitological sedimentation test. The traceability records of the positives were checked, for identifying autochthonous cases. Additionally, the tracking data of cattle slaughtered between the years of 2018 and 2021, which presented liver condemnations, were analyzed for defining autochthonous cases of bovine fasciolosis. In total, there were fecal samplings in 106 farms, with 1,927 tested cattle. From these, 178 (9.2%) were positive and autochthonous, but the overall result did not reflect the heterogeneity found between the evaluated municipalities. During the analyzed period, 11,556 cattle were slaughtered, with 1,744 (15.1%) liver condemnations due to fasciolosis in animals that never left the Serrana Mesoregion. The present study confirmed the presence of autochthonous cases of bovine fasciolosis in municipalities of the Serrana Mesoregion, and verified a high rate of positivity in animals slaughtered in the regional slaughterhouses. When the prevalence of bovine fasciolosis in vivo was compared with the postmortem positivity index in five municipalities, the results disagreed in four municipalities (p-value < 0.05), emphasizing the need for field investigation for the better understanding of the distribution and frequency of the parasitosis.
Selection of internal reference gene for normalization of reverse transcription-quantitative polymerase chain reaction analysis in Mycoplasma hyopneumoniae
Authors: Li Shiyang , Zhou Yanqing , Yuan Ting , Feng Zhixin , Zhang Zhenzhen , Wu Yuzi , Xie Qingyun , Wang Jia , Li Quan , Deng Zhibang , Yu Yanfei , Yuan Xiaomin
Published: July 2022

Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), which resulting in considerable economic losses in pig farming globally. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a major tool for gene expression studies. However, no internal reference genes for normalization of RT-qPCR data of M. hyopneumoniae have been reported. The aim of this study was to screen the most stable genes for RT-qPCR analysis in M. hyopneumoniae under different conditions. Therefore, a total of 13 candidate internal reference genes (rpoC, Lipo, sgaB, oppB, hypo621, oppF, gyrB, uvrA, P146, prfA, proS, gatB, and hypo499) of M. hyopneumoniae filtered according to the reported quantitative proteomic analysis and the 16S rRNA internal reference gene frequently used in other bacteria were selected for RT-qPCR analysis. The mRNAs from different virulence strains (168, 168 L, J, NJ, and LH) at five different growth phases were extracted. The corresponding cycle threshold (Ct) values of the 25 reverse transcribed cDNAs using the 14 candidate genes were determined. Different internal reference genes or combinations were then screened for expression stability analysis using various statistical tools and algorithms, including geNorm, BestKeeper, and NormFinder software, to ensure the reliability of the analysis. Through further comprehensive evaluation of the RefFinder software, it is concluded that the gatB gene was the most suitable internal reference gene for samples of the different virulence strains in different growth phases for M. hyopneumoniae, followed by prfA, hypo499, and gyrB.
Foot-and-mouth disease virus VP1 promotes viral replication by regulating the expression of chemokines and GBP1
Authors: Yang Li , Chen Hong , Liu Liqing , Song Jingjing , Feng Tian , Li Yihan , Shen Chao , Kong Lingbao , Xin Xiu
Published: July 2022

Foot-and-mouth disease virus (FMDV) is an acute, highly contagious, and economically destructive pathogen of vesicular disease that affects domestic and wild cloven-hoofed animals. The FMDV VP1 protein is an important part of the nucleocapsid and plays a significant role during FMDV infection. However, the signal pathways mediated by VP1 in the life cycle of FMDV and the related mechanisms are not yet fully understood. Here, we performed RNA-seq to compare gene expression profiles between pCAGGS-HA-VP1 transfected PK-15 cells and pCAGGS-HA (empty vector) transfected PK-15 cells. The results showed 5,571 genes with significantly different expression levels, of which 2,981 were up-regulated and 2,590 were down-regulated. GO enrichment analysis showed that 51 GO terms were significantly enriched in cell components including protein complex, membrane and organelle part. KEGG enrichment analysis showed 11 KEGG pathways were significantly enriched which were mainly related to the immune system, infectious viral disease, and signal transduction. Among the up-regulated genes, the chemokines such as CCL5, CXCL8, and CXCL10 in turn promoted FMDV replication. In contrast, GBP1, an interferon-stimulated gene that was suppressed by VP1 and FMDV, could effectively inhibit FMDV replication. Our research provides a comprehensive overview of the response of host cells to VP1 protein and a basis for further research to understand the roles of VP1 in FMDV infection including the genes involved in FMDV replication.
Active learning for an evidence-based veterinary medicine course during COVID-19
Authors: St-Hilaire Sophie , Nekouei Omid , Parkes Rebecca S. V. , Rosanowski Sarah M.
Published: July 2022

Epidemiology is often a challenging course that is not well appreciated by many students learning veterinary medicine. The curriculum for this topic can sometimes be dry, difficult for students to contextualize, and heavy with statistics and mathematical concepts. We incorporated the concepts of epidemiology that are most important for practicing veterinarians and combined these with evidence-based veterinary medicine principles to create a practical course for second-year undergraduate veterinary students. We share the structure of our course and the different learning components, which also included incorporating graduate student mentors for journal clubs and an assignment that culminated in some students publishing their review findings. Anecdotal responses from students suggest they enjoyed the course and learned skills they felt would be useful in veterinary practice to help them make evidence-based clinical decisions.
Identifying Field Crop Diseases Using Transformer-Embedded Convolutional Neural Network
Authors: Zhu Weidong , Sun Jun , Wang Simin , Shen Jifeng , Yang Kaifeng , Zhou Xin
Published: July 2022

The yield and security of grain are seriously infringed on by crop diseases, which are the critical factor hindering the green and high-quality development of agriculture. The existing crop disease identification models make it difficult to focus on the disease spot area. Additionally, crops with similar disease characteristics are easily misidentified. To address the above problems, this paper proposed an accurate and efficient disease identification model, which not only incorporated local and global features of images for feature analysis, but also improved the separability between similar diseases. First, Transformer Encoder was introduced into the improved model as a convolution operation, so as to establish the dependency between long-distance features and extract the global features of the disease images. Then, Centerloss was introduced as a penalty term to optimize the common cross-entropy loss, so as to expand the inter-class difference of crop disease characteristics and narrow their intra-class gap. Finally, according to the characteristics of the datasets, a more appropriate evaluation index was used to carry out experiments on different datasets. The identification accuracy of 99.62% was obtained on Plant Village, and the balanced accuracy of 96.58% was obtained on Dataset1 with a complex background. It showed good generalization ability when facing disease images from different sources. The improved model also balanced the contradiction between identification accuracy and parameter quantity. Compared with pure CNN and Transformer models, the leaf disease identification model proposed in this paper not only focuses more on the disease regions of leaves, but also better distinguishes different diseases with similar characteristics.
Estimation of Leaf Area Index and Above-Ground Biomass of Winter Wheat Based on Optimal Spectral Index
Authors: Tang Zijun , Guo Jinjin , Xiang Youzhen , Lu Xianghui , Wang Qian , Wang Haidong , Cheng Minghui , Wang Han , Wang Xin , An Jiaqi , Abdelghany Ahmed , Li Zhijun , Zhang Fucang
Published: July 2022

Leaf area index (LAI) and above-ground biomass are both vital indicators for evaluating crop growth and development, while rapid and non-destructive estimation of crop LAI and above-ground biomass is of considerable significance for crop field management. Owing to the advantages of repeatable and high-throughput observations, spectral technology provides a feasible method for obtaining LAI and above-ground biomass of crops. In the present study, the spectral, LAI and above-ground biomass data of winter wheat were collected, and 7 species (14 in total) were calculated based on the original and first-order differential spectrum correlation spectral indices with LAI. Then, the correlation matrix method was used for correlation with LAI. The optimal wavelength combination was extracted, and the results were calculated as the optimal spectral index related to LAI. The calculation process of the optimal spectral index related to above-ground biomass was the same as that aforementioned. Finally, the optimal spectral index was divided into three groups of model input variables, winter wheat LAI and above-ground biomass estimation models were constructed using support vector machine (SVM), random forest (RF) and a back propagation neural network (BPNN), and the models were verified. The results show that the correlation coefficient between the highest of the optimal spectral indices, the LAI, and the above-ground biomass of winter wheat exceeded 0.6, and the correlation was good. The methods for establishing the optimal estimation models for LAI and above-ground biomass of winter wheat are all modeling methods in which the input variables are the combination of the first-order differential spectral index (combination 2) and RF. The R2 of the LAI estimation model validation set was 0.830, the RMSE was 0.276, and the MRE was 6.920; the R2 of the above-ground biomass estimation model validation set was 0.682, RMSE was 235.016, MRE was 4.336, and the accuracies of both models were high. The present research results can provide a theoretical basis for crop monitoring based on spectral technology and provide an application reference for the rapid estimation of crop growth parameters.
Mitochondrial Whole D-Loop Variability in Polish Draft Horses of Sztumski Subtype
Authors: Myćka Grzegorz , Klecel Weronika , Stefaniuk-Szmukier Monika , Jaworska Joanna , Musiał Adrianna Dominika , Ropka-Molik Katarzyna
Published: July 2022

The Polish draft horse (PDH) breed is a result of crossing local mares with imported cold-blooded stallions, such as Belgians, Ardennes, Fjords, and others. A part of the broodmare stock investigated in this study was also imported from various countries, such as Denmark. In this study, we investigate the genetic composition of the PDH by analyzing the whole mitochondrial d-loop variability and comparing it to previously demonstrated whole d-loop sequences of other cold-blooded breeds: Ardennais, Belgian, Breton, Clydesdale, Noriker, Norwegian Fjord, Percheron, and Suffolk. Our results show high nucleotide diversity within the PDH population (π = 0.011), and the existence of two main haplogroups: one of relatively concise origin, with strong kinship to the Belgian breed, and the second showing close relation to the majority of other analyzed cold-blooded breeds. Some of the PDH maternal strains clustered separately, which can be a result of the influence of other unidentified breeds that served as a foundation stock for the present population. This present study explains the genetic relationship of the PDH to other cold-blooded breeds and indicates the high genetic diversity of the breed.
Effect of Folic Acid Supplements on Progesterone Profile and Blood Metabolites of Heat-Stressed Holstein Cows during the Early Stage of Pregnancy
Authors: Kilany Abdelrahman A. , El-Darawany Abdel-Halim A. , El-Tarabany Akram A. , Al-Marakby Khaled M.
Published: July 2022

The aim was to elucidate the impact of oral folic acid (FA) supplements on progesterone profile, blood metabolites and biochemical indices of heat-stressed Holstein cows during the early stage of pregnancy. The study lasted from the day of artificial insemination through the end of the fourth week of pregnancy. The first group (CON, n = 17) received 0 μg of FA/kg BW as a control. The second and third groups received oral FA doses of 5 (FA5, n = 19) and 10 (FA10, n = 20) μg kg−1 BW, respectively. At the 2nd and 3rd weeks of pregnancy, the FA10 group had greater progesterone levels than the CON group (p < 0.05). The FA10 group had a greater progesterone level than the FA5 and CON groups at the fourth week of pregnancy (p < 0.01). The FA10 group had higher folate levels than CON group during the first three weeks of pregnancy (p < 0.01). Both FA-supplemented groups had significantly greater serum folates than the CON group by the end of the fourth week of pregnancy (p < 0.01). At the 2nd and 4th weeks of pregnancy, the FA10 group had greater levels of serum glucose and globulin than the CON group (p = 0.028 and 0.049, respectively). Both FA-supplemented groups had greater serum growth hormone (GH) levels at the 4th week of pregnancy (p = 0.020). Additionally, the FA10 group showed significantly higher levels of IGF-1 at the 2nd and 4th week of gestation (p = 0.040 and 0.001, respectively). FA supplementation decreased the levels of non-esterified fatty acid (NEFA) at the 2nd and 4th week of gestation (p = 0.020 and 0.035, respectively). Additionally, the FA10 group showed significantly higher pregnancy-associated glycoprotein (PAG) levels at the 2nd and 4th week of gestation (p = 0.005 and 0.001, respectively). In conclusion, oral FA supplementation (10 mcg kg−1) in the first month of gestation improved the progesterone profile, as well as blood folates, PAG, GH, and IGF-1 concentrations in heat-stressed Holstein cows. These findings could be useful in developing practical strategies to keep dairy cows’ regular reproductive patterns under heat stress conditions.
Important Mycoses of Wildlife: Emphasis on Etiology, Epidemiology, Diagnosis, and Pathology—A Review: PART 1
Authors: Ugochukwu Iniobong Chukwuebuka Ikenna , Aneke Chioma Inyang , Sani Nuhu Abdulazeez , Omeke Jacinta Ngozi , Anyanwu Madubuike Umunna , Odigie Amienwanlen Eugene , Onoja Remigius Ibe , Ocheja Ohiemi Benjamin , Ugochukwu Miracle Oluchukwu , Luca Iasmina , Makanju Olabisi Aminah
Published: July 2022

In the past few years, there has been a spurred tripling in the figures of fungal diseases leading to one of the most alarming rates of extinction ever reported in wild species. Some of these fungal diseases are capable of virulent infections and are now considered emerging diseases due to the extremely high number of cases diagnosed with fungal infections in the last few decades. Most of these mycotic diseases in wildlife are zoonotic, and with the emergence and re-emergence of viral and bacterial zoonotic diseases originating from wildlife, which are causing devastating effects on the human population, it is important to pay attention to these wildlife-borne mycotic diseases with zoonotic capabilities. Several diagnostic techniques such as fungal isolation, gross pathology, histopathology, histochemistry, cytology, immunohistochemistry, radiography, CT, and molecular methods such as PCR or ELISA have been invaluable in the diagnosis of wildlife mycoses. The most important data used in the diagnosis of these wildlife mycoses with a zoonotic potential have been re-emphasized. This will have implications for forestalling future epidemics of these potential zoonotic mycotic diseases originating from wildlife. In conclusion, this review will highlight the etiology, epidemiology, diagnosis, pathogenesis, pathogenicity, pathology, and hematological/serum biochemical findings of five important mycoses found in wild animals.
Physicochemical Analysis of Mixed Venous and Arterial Blood Acid-Base State in Horses at Core Temperature during and after Moderate-Intensity Exercise
Authors: Lindinger Michael I. , Waller Amanda P.
Published: July 2022

The present study determined the independent contributions of temperature, strong ion difference ([SID]), total weak acid concentration ([Atot]) and PCO2 to changes in arterial and mixed venous [H+] and total carbon dioxide concentration ([TCO2]) during 37 min of moderate intensity exercise (~50% of heart rate max) and the first 60 min of recovery. Six horses were fitted with indwelling carotid and pulmonary artery (PA) catheters, had PA temperature measured, and had blood samples withdrawn for immediate analysis of plasma ion and gas concentrations. The increase in core temperature during exercise (+4.5 °C; p < 0.001) significantly (p < 0.05) increased PO2, PCO2, and [H+], but without a significant effect on [TCO2] (p > 0.01). The physicochemical acid-base approach was used to determine contributions of independent variables (except temperature) to the changes in [H+] and [TCO2]. In both arterial and venous blood, there was no acidosis during exercise and recovery despite significant (p < 0.05) increases in [lactate] and in venous PCO2. In arterial blood plasma, a mild alkalosis with exercise was due to primarily to a decrease in PCO2 (p < 0.05) and an increase in [SID] (p < 0.1). In venous blood plasma, a near absence of change in [H+] was due to the acidifying effects of increased PCO2 (p < 0.01) being offset by the alkalizing effects of increased [SID] (p < 0.05). The effect of temperature on PO2 (p < 0.001) resulted in an increased arterio-venous PO2 difference (p < 0.001) that would facilitate O2 transfer to contracting muscle. The simultaneous changes in the PCO2 and the concentrations of the other independent acid-base variables (contributions from individual strong and weak ions as manifest in [SID] and [Atot]) show complex, multilevel control of acid-base states in horses performing even moderate intensity exercise. Correction of acid-base variables to core body temperature presents a markedly different physiological response to exercise than that provided by variables measured and presented at an instrument temperature of 37 °C.
A 25-Year Retrospective Review of Mortality in Chimpanzees (Pan troglodytes) in Accredited U.S. Zoos from a Management and Welfare Perspective
Authors: Ross Stephen R. , Joshi Priyanka B. , Terio Karen A. , Gamble Kathryn C.
Published: July 2022

Understanding causes of death allows adjustment of health management strategies for animals in managed care. From 224 documented chimpanzee deaths occurring from 1995 to 2019 in 42 accredited U.S. zoos, post-mortem records and necropsy reports were analyzed for the primary cause of death, which were available for 214 individuals. In total, 37 cases of stillbirth and neonatal deaths were assessed (16.5%); however, the focus was otherwise placed on the remaining 177 cases in which the death occurred in individuals aged greater than 1 month. There were no sex-related differences in etiology; however, age variation in the cause of death was statistically significant (p < 0.001). Elderly (35 years and older) chimpanzees tended to die of intrinsic, often degenerative, etiologies, whereas infants, juveniles, and adolescents (less than 15 years) were more likely to be involved in fatal trauma. Overall, there were 27 deaths (15.3% of all post-neonatal deaths) related to trauma and 13 of these were directly or indirectly related to conspecific aggression. Understanding causes of mortality and the interrelation with management can benefit managed populations of chimpanzees.
Evaluation of Predisposing Factors of Necrotic Enteritis in Experimentally Challenged Broiler Chickens
Authors: Justino Larissa , Baptista Ana A. S. , Souza Marielen de , Menck-Costa Maísa F. , Pires Bárbara G. , Cicero Claudineia E. , Bracarense Ana P. F. R. L. , Kaneko Vanessa M. , Oba Alexandre , Okamoto Adriano S. , Andreatti Filho Raphael L.
Published: July 2022

Clostridium perfringens is the etiological agent of NE, a disease that greatly affects the poultry industry. Experiments on the induction of NE are difficult to carry out, as it is a multifactorial disease, and thus different predisposing factors have been used. This study evaluated the effect of the Gumboro disease vaccine virus vaccine (IBDV-vac) associated or not with infection by Eimeria spp. in broilers, as a predisposing factor for NE. Broilers (n = 99) were divided into groups (11) challenged with IBDV-vac, Eimeria spp. CP type G (CP13, CP14 and CP03) or both. The macroscopic evaluation revealed that the highest average (3.45) of injury occurred for the CP13 + IBDV-vac group. The microscopic analysis showed that Eimeria spp. increased the population of intraepithelial lymphocytes and reduced the villus/crypt ratio in duodenum and jejunum when associated with CP13 or CP14. There was a synergistic effect between the CP strain used and the predisposing factors; nevertheless, it was not clear which was the most effective predisposing factor to potentiate the lesions, suggesting that the association of the strain with the factors should first be evaluated for each experimental protocol.
The g.4290 C>G Polymorphism in the FADS2 Gene Modifies the Fatty Acid Profile of the Pectoralis Superficialis Muscle of Ross 308 Broiler Chickens
Authors: Kaczor Urszula , Sawicki Sebastian , Nowak Joanna , Gabryś Julia , Jurczyk Jakub , Wojtysiak Dorota , Połtowicz Katarzyna
Published: July 2022

The effect of the g.4290 C>G substitution in the FADS2 gene and g.285 C>T in the FABP4 gene on carcass quality, meat quality, and fatty acid profile of the pectoralis superficialis muscle of 238 male broiler chickens reared up to 45 days of age was analyzed. A significant influence of g.4290 C>G in the FADS2 gene on the pectoralis superficialis muscle fatty acid profile was demonstrated. Chickens with the GG genotype were characterized by the highest content of conjugated linoleic acid, amino acids, eicosapentaenoic acids, docosapentaenoic acid, docosahexaenoic acids. and the lowest value of the linoleic acid/alpha-linolenic acid ratio. The FABP4 polymorphism determined only the content of C18:1n-9, C18:2n-6 and docosahexaenoic acid. There was no effect of the studied genotypes on final body weight, carcass quality traits, or quality of broiler pectoral muscles.
Antioxidant Effects of DPP-4 Inhibitors in Early Stages of Experimental Diabetic Retinopathy
Authors: Ramos Hugo , Bogdanov Patricia , Huerta Jordi , Deàs-Just Anna , Hernández Cristina , Simó Rafael
Published: July 2022

Hyperglycemia-induced oxidative stress plays a key role in the impairment of the retinal neurovascular unit, an early event in the pathogenesis of DR. The aim of this study was to assess the antioxidant properties of topical administration (eye drops) of sitagliptin in the diabetic retina. For this purpose, db/db mice received sitagliptin or vehicle eye drops twice per day for two weeks. Age-matched db/+ mice were used as the control group. We evaluated retinal mRNA (RT-PCR) and protein levels (Western blotting and immunohistochemistry) of different components from both the antioxidant system (NRF2, CAT, GPX, GR, CuZnSOD, and MnSOD) and the prooxidant machinery (PKC and TXNIP). We also studied superoxide levels (dihydroethidium staining) and oxidative damage to DNA/RNA (8-hydroxyguanosine immunostaining) and proteins (nitrotyrosine immunostaining). Finally, NF-кB translocation and IL-1β production were assessed through Western blotting and/or immunohistochemistry. We found that sitagliptin protected against diabetes-induced oxidative stress by reducing superoxide, TXNIP, PKC, and DNA/RNA/protein oxidative damage, and it prevented the downregulation of NRF2 and antioxidant enzymes, with the exception of catalase. Sitagliptin also exerted anti-inflammatory effects, avoiding both NF-кB translocation and IL-1β production. Sitagliptin prevents the diabetes-induced imbalance between ROS production and antioxidant defenses that occurs in diabetic retinas.
Oxidative Stress in Tauopathies: From Cause to Therapy
Authors: Bartolome Fernando , Carro Eva , Alquezar Carolina
Published: July 2022

Oxidative stress (OS) is the result of an imbalance between the production of reactive oxygen species (ROS) and the antioxidant capacity of cells. Due to its high oxygen demand, the human brain is highly susceptible to OS and, thus, it is not a surprise that OS has emerged as an essential component of the pathophysiology of several neurodegenerative diseases, including tauopathies. Tauopathies are a heterogeneous group of age-related neurodegenerative disorders characterized by the deposition of abnormal tau protein in the affected neurons. With the worldwide population aging, the prevalence of tauopathies is increasing, but effective therapies have not yet been developed. Since OS seems to play a key role in tauopathies, it has been proposed that the use of antioxidants might be beneficial for tau-related neurodegenerative diseases. Although antioxidant therapies looked promising in preclinical studies performed in cellular and animal models, the antioxidant clinical trials performed in tauopathy patients have been disappointing. To develop effective antioxidant therapies, the molecular mechanisms underlying OS in tauopathies should be completely understood. Here, we review the link between OS and tauopathies, emphasizing the causes of OS in these diseases and the role of OS in tau pathogenesis. We also summarize the antioxidant therapies proposed as a potential treatment for tauopathies and discuss why they have not been completely translated to clinical trials. This review aims to provide an integrated perspective of the role of OS and antioxidant therapies in tauopathies. In doing so, we hope to enable a more comprehensive understanding of OS in tauopathies that will positively impact future studies.
Evaluation of a Dietary Grape Extract on Oxidative Status, Intestinal Morphology, Plasma Acute-Phase Proteins and Inflammation Parameters of Weaning Piglets at Various Points of Time
Authors: Rajković Emina , Schwarz Christiane , Kapsamer Stefan Bruno , Schedle Karl , Reisinger Nicole , Emsenhuber Caroline , Ocelova Vladimira , Roth Nataliya , Frieten Dörte , Dusel Georg , Gierus Martin
Published: July 2022

Reports of the underlying mechanisms of dietary grape extract (GE) in overcoming weaning challenges in piglets have been partly inconsistent. Furthermore, evaluations of the effects of GE at weaning in comparison to those of widely used therapeutic antibiotics have been scarce. To explore the mode of action of GE in selected tissues and plasma, we evaluated gut morphology, antioxidant and inflammation indices. Accordingly, 180 weaning piglets were allocated to three treatment groups: negative control (NC), NC and antibiotic treatment for the first 5 days of the trial (positive control, PC), and NC and GE (entire trial). The villus surface was positively affected by GE and PC on day 27/28 of the trial in the jejunum and on day 55/56 of the trial in the ileum. In the colon, NC tended (p < 0.10) to increase crypt parameters compared to PC on day 55/56. The PC group tended (p < 0.10) to increase catalase activity in the ileum and decrease Cu/Zn-SOD activity in the jejunum, both compared to NC. There were no additional effects on antioxidant measurements of tissue and plasma, tissue gene expression, or plasma acute-phase proteins. In conclusion, GE supplementation beneficially affected the villus surface of the small intestine. However, these changes were not linked to the antioxidant and anti-inflammatory properties of GE.
Influences of Breathing Exercises and Breathing Exercise Combined with Aerobic Exercise on Changes in Basic Spirometry Parameters in Patients with Bronchial Asthma
Authors: Grznár Ľuboš , Sucháň Dávid , Labudová Jana , Odráška Lukáš , Matúš Ivan
Published: July 2022

Scientific evidence shows that breathing or aerobic programs can improve the quality of life of asthma patients. The aim of this work was to find out the influences of breathing exercises and breathing exercises combined with aerobic exercise on changes in spirometry parameters in patients with bronchial asthma. Participants: The group consisted of 33 women with bronchial asthma—mild to moderate persistent levels of FEV1 reduction (80–50%)—with a mean age of 34.73 ± 1.53 years. They were randomly assigned to experimental group 1 (EX1), experimental group 2 (EX2) or the control group (CG). Materials and methods: Changes in spirometry parameters were evaluated over a 16-week period in the three groups: CG (placebo), EX1 (breathing exercises) and EX 2 (combination of breathing exercises with an aerobic program). To evaluate the pre-training and post-training diagnostics, we used MIR Spirobank II. The influences of the experimental and control factors were assessed using the following dependent variables: forced vital capacity (FVC), forced expiratory volume in one second (FEV1), Tiffeneau–Pinelli index (FEV1/FVC ratio), peak expiratory flow (PEF) and forced mid-expiratory flow (FEF25–75%). We used the Wilcoxon t-test and the Kruskal–Wallis test to evaluate the differences in the measured parameters. To examine the effect of our protocols, we used effect size (ES). Results: In CG we observed improvements in: FVC—(5%; p < 0.05; ES = 0.437). FEV1—(7.33%; p < 0.01; ES = 0.585). FEV1/FVC ratio (5.27%; p < 0.01; ES = 0.570). PEF (11.22%; p < 0.01; ES = 0.448). FEF25–75% (7.02%; p < 0.01; ES = 0.628). In EX1 we observed improvements in: FVC (5.23%; p < 0.01; ES = 0.631), FEV1 (20.67%; p < 0.01; ES = 0.627), FEV1/FVC ratio (16.06%; p < 0.01; ES = 0.628), PEF (13.35%; p < 0.01; ES = 0.627) and FEF25–75% (13.75%; p < 0.01; ES = 0.607). In EX2 we observed improvements in: FVC (9.12%; p < 0.01; ES = 0.627), FEV1 (27.37%; p < 0.01; ES = 0.626), FEV1/FVC ratio (15.32%; p < 0.01; ES = 0.610), PEF (30.66%; p < 0.01; ES = 0.626) and FEF25–75% (58.99%; p < 0.01; ES = 0.626). Significant differences compared to the control group were observed in EX1 for FEV1 (p < 0.05) and FEV1/FVC ratio (p < 0.01); and in EX2 for FEV1 (p < 0.05), FEV1/FVC ratio (p < 0.01), PEF (p < 0.05) and FEF (p < 0.05). A significant difference between EX1 and EX2 was observed in PEF (p < 0.05). Conclusions: It appears to be that combination of breathing exercises with aerobic activities is a more beneficial option for patients with bronchial asthma.
Enhanced Adhesion—Efficient Demolding Integration DLP 3D Printing Device
Authors: Jiang Ting , Yan Bo , Jiang Minzheng , Xu Buguang , Xu Yi , Yu Yueqiang , Ma Tingang , Wang Hao
Published: July 2022

A novel forming method of enhanced adhesion-efficient demolding integration is proposed to solve the problems of weak adhesion between the initial forming layer and the printing platform as well as the excessive stripping force at the bottom of the liquid tank when the printing platform rises. Therefore, a digital light processing (DLP) 3D printing forming device equipped with a porous replaceable printing platform and a swing mechanism for the liquid tank is manufactured and verified by experiments. The experimental results show that the porous printing platform can enhance the adhesion between the initial forming layer and the printing platform and improve the demolding efficiency of the forming device. In addition, the pull-out design of the printing platform plate reduces the maintenance cost of the forming device. Therefore, the device has a good application prospect.