bluuebunny/crossref_metadata_2025 huggingface dataset:
Cross-linking by protein oxidation in the rapidly setting gel-based glues of slugs
Authors: Bradshaw Andrew , Salt Michael , Bell Ashley , Zeitler Matt , Litra Noelle , Smith Andrew M.
Published: April 2011

The terrestrial slug Arion subfuscus secretes a glue that is a dilute gel with remarkable adhesive and cohesive strength. The function of this glue depends on metals, raising the possibility that metal-catalyzed oxidation plays a role. The extent and time course of protein oxidation was measured by immunoblotting to detect the resulting carbonyl groups. Several proteins, particularly one with a relative molecular mass (Mr) of 165×103, were heavily oxidized. Of the proteins known to distinguish the glue from non-adhesive mucus, only specific size variants were oxidized. The oxidation appears to occur within the first few seconds of secretion. Although carbonyls were detected by 2,4-dinitrophenylhydrazine (DNPH) in denatured proteins, they were not easily detected in the native state. The presence of reversible cross-links derived from carbonyls was tested for by treatment with sodium borohydride, which would reduce uncross-linked carbonyls to alcohols, but stabilize imine bonds formed by carbonyls and thus lead to less soluble complexes. Consistent with imine bond formation, sodium borohydride led to a 20–35% decrease in the amount of soluble protein with a Mr of 40–165 (×103) without changing the carbonyl content per protein. In contrast, the nucleophile hydroxylamine, which would competitively disrupt imine bonds, increased protein solubility in the glue. Finally, the primary amine groups on a protein with a Mr of 15×103 were not accessible to acid anhydrides. The results suggest that cross-links between aldehydes and primary amines contribute to the cohesive strength of the glue.
Efficacy of Agents to Prevent and Treat Enteral Feeding Tube Clogs
Authors: Dandeles Lauren M , Lodolce Amy E
Published: April 2011

Objective:

To review the efficacy and safety of agents used to prevent and treat clog formation in enteral feeding tubes.

Data Sources:

A literature search was conducted through MEDLINE (1948-February 2011) and International Pharmaceutical Abstracts (1970-February 2011) using the search terms enteral feeding tube and occlusion. In addition, reference citations from publications identified were reviewed.

Study Selection and Data Extraction:

All English-language publications were reviewed for applicability.

Data Synthesis:

Occlusion is a common complication of enteral tube feeding. With Food and Drug Administration regulations, pancreatic enzymes have recently been reformulated and previously published reports can no longer be applied to currently available agents. This has led to concern over what available products have been shown to be efficacious. Three in vitro studies, 1 randomized in vivo trial, and 1 descriptive report were reviewed. In the prevention of tube clogging, it was concluded that water was comparable in efficacy, while being more readily available and cost effective, when compared to Coca-Cola, and both were superior to cranberry juice. For resolution of an existing clog, evidence of the efficacy of any individual agents is limited and has not been reproducible. New formulations of pancreatic enzymes, a new delivery system for enzymes, and products to mechanically dismantle clogs have become commercially available, but no studies have been completed to evaluate safety and efficacy. Comparative in vivo studies of currently available products are needed to evaluate possible methods for resolving an occlusion.

Conclusions:

Water flushes have shown to be the most effective method in preventing enteral feeding tube clogging. Well-designed trials are needed to establish the proper place in therapy of new commercially available agents marketed for resolving clogs. In addition, well-designed in vivo trials are needed to establish the role, dosage, and extemporaneous formulation of extended-release pancreatic enzymes in treating such clogs.
Role of α2-Agonists in the Treatment of Acute Alcohol Withdrawal
Authors: Muzyk Andrew J , Fowler Jill A , Norwood Daryn K , Chilipko Allison
Published: April 2011

Objective:

To evaluate literature reporting on the role of norepinephrine in alcohol withdrawal and to determine the safety and efficacy of α2-agonists in reducing symptoms of this severe condition.

Data Sources:

Articles evaluating the efficacy and safety of the α2-agonists clonidine and dexmedetomidine were identified from an English-language MEDLINE search (1966-December 2010). Key words included alcohol withdrawal, delirium tremens, clonidine, dexmedetomidine, α2-agonist, norepinephrine, and sympathetic overdrive.

Study Selection and Data Extraction:

Studies that focused on the safety and efficacy of clonidine and dexmedetomidine in both animals and humans were selected.

Data Synthesis:

The noradrenergic system, specifically sympathetic overdrive during alcohol withdrawal, may play an important role in withdrawal symptom development. Symptoms of sympathetic overdrive include anxiety, agitation, elevated blood pressure, tachycardia, and tremor. Therefore, α2-agonists, which decrease norepinephine release, may have a role in reducing alcohol withdrawal symptoms. The majority of controlled animal and human studies evaluated clonidine, but the most recent literature is from case reports on dexmedetomidine. The literature reviewed here demonstrate that these 2 α2-agonists safely and effectively reduce symptoms of sympathetic overdrive and concomitant medication use. Dexmedetomidine may offer an advantage over current sedative medications used in the intensive care unit, such as not requiring intubation with its use, and therefore further study is needed to fully elicit its benefit in alcohol withdrawal.

Conclusion:

Clonidine and dexmedetomidine may provide additional benefit in managing alcohol withdrawal by offering a different mechanism of action for targeting withdrawal symptoms. Based on literature reviewed here, the primary role for clonidine and dexmedetomidine is as adjunctive treatment to benzodiazepines, the standard of care in alcohol withdrawal.
Vancomycin-Induced Neutropenia: Is it Dose- or Duration-Related?
Authors: Black Emily , Lau Tim TY , Ensom Mary HH
Published: April 2011

Objective:

To systematically evaluate the literature to determine whether vancomycin-induced neutropenia is dose- or duration-related and provide clinicians with feasible treatment alternatives.

Data Sources:

A literature search of PubMed (1949-November 2010), MEDLINE (1950-November 2010), EMBASE (1980-November 2010), and International Pharmaceutical Abstracts (1970-November 2010) was performed using the terms vancomycin, neutropenia, and leukopenia. Citations from publications were reviewed for additional references.

Study Selection and Data Extraction:

Studies and case reports were included if they reported neutropenia with vancomycin administration and excluded if they did not describe vancomycin dosages and/or concentrations, or if neutropenia resolved white the patient was still receiving vancomycin. Cases with significant confounders and those in which authors provided minimal information about patients were also excluded.

Data Synthesis:

Seven retrospective chart reviews (ie, case series) and 33 case reports were identified. Of these, 3 retrospective reviews and 26 case reports met inclusion criteria. To our knowledge, no prospective studies have assessed this clinical complication. Data suggest that vancomycin-induced neutropenia may not be completely related to daily dosages, total cumulative dosage, or supratherapeutic vancomycin concentrations. Furthermore, evidence suggests that neutropenia is more likely associated with therapy longer than 7 days, with the majority of episodes occurring beyond 20 days of therapy. Given these findings, a practical approach is to monitor white blood cell (WBC) count with a differential (including absolute neutrophil count) once a week in patients who are receiving vancomycin for more than 7 days.

Conclusions:

Vancomycin-induced neutropenia is most likely associated with prolonged vancomycin exposure. Patients receiving vancomycin for longer than 7 days should have WBC count, differential, monitored weekly. Vancomycin should be discontinued if there is a high clinical suspicion of it causing neutropenia, and an alternative agent should be initiated. Prospective case-controlled studies are needed to better characterize this adverse event.
Assessment of Vancomycin Dosing and Subsequent Serum Concentrations in Pediatric Patients
Authors: Eiland Lea S , English Thomas M , Eiland Edward H
Published: April 2011

Background

Because of concerns regarding increasing microbial resistance to vancomycin, adult treatment guidelines recommend higher trough concentrations based on the type of infectious process. Although these recommendations are not specific to pediatrics, the principles can be extrapolated. Desired higher trough serum concentrations will require escalated dosages of vancomycin in children.

Objective:

To evaluate current dosing regimens and subsequent trough serum concentrations of vancomycin in children, compare these to reference recommended dosages and guidelines, and predict a dosing equation to achieve desired serum concentrations.

Methods:

Pharmacokinetic parameters of children in a community teaching hospital who were prescribed vancomycin from January 2005 to May 2010 were evaluated in this retrospective chart review. Vancomycin dosing and subsequent serum concentrations were analyzed. Therapeutic serum concentrations were evaluated and compared to vancomycin prescribing and monitoring guidelines by year.

Results:

Four hundred thirty-five trough serum concentrations determined in 295 patients were analyzed. The average dosages, when evaluated by year, were 48 mg/kg/day (2005-2008) and 59 mg/kg/day (2009-2010). Using trough concentration recommendations of 5-15 mg/L, vancomycin regimens provided therapeutic trough concentrations 78% of the time from 2005 to 2008. Using 10-20 mg/L as the trough recommendations in 2009-2010, only 49% of serum concentrations reached a therapeutic level. Based on our predictive equation for children aged 1 month-18 years with normal renal function, a vancomycin dosage of 70 mg/kg/day is required to provide trough serum concentrations of 10 mg/L; a dosage of 85 mg/kg/day is required to provide trough serum concentrations of 15 mg/L.

Conclusions:

Our institution was primarily using vancomycin dosing regimens that were recommended in pediatric references (40-60 mg/kg/day), which resulted in subtherapeutic serum concentrations in our population based on new monitoring recommendations. Considering that the currently desired therapeutic trough concentrations of vancomycin are 10-20 mg/L, the total daily dosage should be increased.
Estimates of Statin Discontinuation Rates are Influenced by Exposure and Outcome Definitions
Authors: Geers Harm CJ , Bouvy Marcel L , Heerdink Eibert R
Published: April 2011

Background

Estimates of statin discontinuation rates are generally high but show large variations. Discontinuation rates are possibly influenced by unrecognized supplies from previous prescriptions and the operational definitions of statin discontinuation.

Objective:

To investigate whether the outcome (discontinuation) was affected by (1) the operational definition used to calculate statin exposure and (2) the operational definition and different cutoff values used to calculate discontinuation.

Methods:

Data for this study were obtained from the PHARMO medical record linkage system in the Netherlands. Participants were patients with a new statin and no statin prescription in the preceding year. The outcome, discontinuation, was defined based on a variable number of days without medication after exposure (gaps) or based on the availability of supplies 12 months after the inclusion date (at 1 year). Exposure to statins was assessed by 2 methods. The method termed No Overlap accounted for only the supplies of the last prescription to calculate exposure, and the method termed Overlap accounted for all supplies from previous prescriptions. We investigated the effect of 4 exposure outcome combinations on statin discontinuation estimates.

Results:

The exposure outcome combinations, including overlap, resulted in a 7% unit lower discontinuation rate. At gap lengths of 90 days and longer, no significant differences between No Overlap and Overlap were observed. Shorter minimum gap lengths gave higher discontinuation rates compared to longer minimum gap lengths and ranged from as high as 86% to 21%.

Conclusions:

If previous supplies are accounted for in the calculation of exposure to statins, lower discontinuation rates are observed, The influence of previous supplies on discontinuation rates is less pronounced than the influence of gap lengths. The calculation of exposure does not influence discontinuation if gaps longer than 90 days are used to assess discontinuation.
Defining the Minimal Detectable Change in Scores on the Eight-Item Morisky Medication Adherence Scale
Authors: Muntner Paul , Joyce Cara , Holt Elizabeth , He Jiang , Morisky Donald , Webber Larry S , Krousel-Wood Marie
Published: April 2011

Background

Self-report scales are used to assess medication adherence. Data on how to discriminate change in self-reported adherence over time from random variability are limited.

Objective:

To determine the minimal detectable change for scores on the 8-item Morisky Medication Adherence Scale (MMAS-8).

Methods:

The MMAS-8 was administered twice, using a standard telephone script, with administration separated by 14-22 days, to 210 participants taking antihypertensive medication in the CoSMO (Cohort Study of Medication Adherence among Older Adults). MMAS-8 scores were calculated and participants were grouped into previously defined categories (<6, 6 to <8, and 8 for low, medium, and high adherence).

Results:

The mean (SD) age of participants was 78.1 (5.8) years, 43.8% were black, and 68.1% were women. Overall, 8.1% (17/210), 16.2% (34/210), and 51.0% (107/210) of participants had low, medium, and high MMAS-8 scores, respectively, at both survey administrations (overall agreement 75.2%; 158/210). The weighted κ statistic was 0.63 (95% CI 0.53 to 0.72). The intraclass correlation coefficient was 0.78. The within-person standard error of the mean for change in MMAS-8 scores was 0.81, which equated to a minimal detectable change of 1.98 points. Only 4.3% (9/210) of the participants had a change in MMAS-8 of 2 or more points between survey administrations.

Conclusions:

Within-person changes in MMAS-8 scores of 2 or more points over time may represent a real change in antihypertensive medication adherence.
Cloning and functional analysis of the dpm2 and dpm3 genes from Trichoderma reesei expressed in a Saccharomyces cerevisiae dpm1Δ mutant strain
Authors: Zembek Patrycja , Perlińska-Lenart Urszula , Rawa Katarzyna , Górka-Nieć Wioletta , Palamarczyk Grażyna , Kruszewska Joanna S.
Published: April 2011

InTrichoderma reesei, dolichyl phosphate mannose (dpm) synthase, a key enzyme in the O-glycosylation process, requires three proteins for full activity. In this study, thedpm2anddpm3genes coding for the DPMII and DPMIII subunits ofT. reeseiDPM synthase were cloned and functionally analyzed after expression in theSaccharomyces cerevisiae dpm1Δ[genotype (BY4743;his3Δ1; /leu2Δ0; lys2Δ0; /ura3Δ0; YPR183w::kanMX4] mutant. It was found that apart from the catalytic subunit DPMI, the DPMIII subunit is also essential to form an active DPM synthase in yeast. Additional expression of the DPMII protein, considered to be a regulatory subunit of DPM synthase, decreased the enzymatic activity. We also characterizedS. cerevisiaestrains expressing thedpm1,2,3ordpm1, 3genes and analyzed the consequences ofdpmexpression on protein O-glycosylationin vivoand on the cell wall composition.
Integrin Activation Promotes Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans by Enhancing Integrin Signaling
Authors: Tan Chin Lik , Kwok Jessica C. F. , Patani Rickie , ffrench-Constant Charles , Chandran Siddharthan , Fawcett James W.
Published: April 2011

Chondroitin sulfate proteoglycans (CSPGs) are upregulated after CNS lesions, where they inhibit axon regeneration. In order for axon growth and regeneration to occur, surface integrin receptors must interact with surrounding extracellular matrix molecules. We have explored the hypothesis that CSPGs inhibit regeneration by inactivating integrins and that forcing integrins into an active state might overcome this inhibition. Using cultured rat sensory neurons, we show that the CSPG aggrecan inhibits laminin-mediated axon growth by impairing integrin signaling via decreasing phosphorylated FAK (pFAK) and pSrc levels, without affecting surface integrin levels. Forcing integrin activation and signaling by manganese or an activating antibody TS2/16 reversed the inhibitory effect of aggrecan on mixed aggrecan/laminin surfaces, and enhanced axon growth from cultured rat sensory neurons (manganese) and human embryonic stem cell-derived motoneurons (TS2/16). The inhibitory effect of Nogo-A can also be reversed by integrin activation. These results suggest that inhibition by CSPGs can act via inactivation of integrins, and that activation of integrins is a potential method for improving axon regeneration after injury.
The Canonical Nuclear Factor-κB Pathway Regulates Cell Survival in a Developmental Model of Spinal Cord Motoneurons
Authors: Mincheva Stefka , Garcera Ana , Gou-Fabregas Myriam , Encinas Mario , Dolcet Xavier , Soler Rosa M.
Published: April 2011

In vivoandin vitromotoneuron survival depends on the support of neurotrophic factors. These factors activate signaling pathways related to cell survival or inactivate proteins involved in neuronal death. In the present work, we analyzed the involvement of the nuclear factor-κB (NF-κB) pathway in mediating mouse spinal cord motoneuron survival promoted by neurotrophic factors. This pathway comprises ubiquitously expressed transcription factors that could be activated by two different routes: the canonical pathway, associated with IKKα/IKKβ kinase phosphorylation and nuclear translocation RelA (p65)/p50 transcription factors; and the noncanonical pathway, related to IKKα kinase homodimer phosphorylation and RelB/p52 transcription factor activation. In our system, we show that neurotrophic factors treatment induced IKKα and IKKβ phosphorylation and RelA nuclear translocation, suggesting NF-κB pathway activation. Protein levels of different members of the canonical or noncanonical pathways were reduced in a primary culture of isolated embryonic motoneurons using an interference RNA approach. Even in the presence of neurotrophic factors, selective reduction of IKKα, IKKβ, or RelA proteins induced cell death. In contrast, RelB protein reduction did not have a negative effect on motoneuron survival. Together these results demonstrated that the canonical NF-κB pathway mediates motoneuron survival induced by neurotrophic factors, and the noncanonical pathway is not related to this survival effect. Canonical NF-κB blockade induced an increase of Bim protein level and apoptotic cell death. Bcl-xLoverexpression or Bax reduction counteracted this apoptotic effect. Finally, RelA knockdown causes changes of CREB and Smn protein levels.
Chromatic Bipolar Cell Pathways in the Mouse Retina
Authors: Breuninger Tobias , Puller Christian , Haverkamp Silke , Euler Thomas
Published: April 2011

Like most mammals, mice feature dichromatic color vision based on short (S) and middle (M) wavelength-sensitive cone types. It is thought that mammals share a retinal circuit that in dichromats compares S- and M-cone output to generate blue/green opponent signals, with bipolar cells (BCs) providing separate chromatic channels. Although S-cone-selective ON-BCs (type 9 in mouse) have been anatomically identified, little is known about their counterparts, the M-cone-selective OFF-BCs. Here, we characterized cone connectivity and light responses of selected mouse BC types using immunohistochemistry and electrophysiology. Our anatomical data indicate that four (types 2, 3a/b, and 4) of the five mouse OFF-BCs indiscriminately contact both cone types, whereas type 1 BCs avoid S-cones. Light responses showed that the chromatic tuning of the BCs strongly depended on their position along the dorsoventral axis because of the coexpression gradient of M- and S-opsin found in mice. In dorsal retina, where coexpression is low, most type 2 cells were green biased, with a fraction of cells (≈14%) displaying strongly blue-biased responses, likely reflecting S-cone input. Type 1 cells were also green biased but did not comprise blue-biased “outliers,” consistent with type 1 BCs avoiding S-cones. We therefore suggest that type 1 represents the green OFF pathway in mouse. In addition, we confirmed that type 9 BCs display blue-ON responses. In ventral retina, all BC types studied here displayed similar blue-biased responses, suggesting that color vision is hampered in ventral retina. In conclusion, our data support an antagonistically organized blue/green circuit as the common basis for mammalian dichromatic color vision.
Nucleus Accumbens μ-Opioid Receptors Mediate Social Reward
Authors: Trezza Viviana , Damsteegt Ruth , Achterberg E. J. Marijke , Vanderschuren Louk J. M. J.
Published: April 2011

Positive social interactions are essential for emotional well-being and proper behavioral development of young individuals. Here, we studied the neural underpinnings of social reward by investigating the involvement of opioid neurotransmission in the nucleus accumbens (NAc) in social play behavior, a highly rewarding social interaction in adolescent rats. Intra-NAc infusion of morphine (0.05–0.1 μg) increased pinning and pouncing, characteristic elements of social play behavior in rats, and blockade of NAc opioid receptors with naloxone (0.5 μg) prevented the play-enhancing effects of systemic morphine (1 mg/kg, s.c.) administration. Thus, stimulation of opioid receptors in the NAc was necessary and sufficient for morphine to increase social play. Intra-NAc treatment with the selective μ-opioid receptor agonist [d-Ala2,N-MePhe4,Gly5-ol]enkephalin (DAMGO) (0.1–10 ng) and the μ-opioid receptor antagonist Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH2(CTAP) (0.3–3 μg) increased and decreased social play, respectively. The δ-opioid receptor agonist DPDPE ([d-Pen2,d-Pen5]-enkephalin) (0.3–3 μg) had no effects, whereas the κ-opioid receptor agonist U69593 (N-methyl-2-phenyl-N-[(5R,7S,8S)-7-(pyrrolidin-1-yl)-1-oxaspiro[4.5]dec-8-yl]acetamide) (0.01–1 μg) decreased social play. Intra-NAc treatment with β-endorphin (0.01–1 μg) increased social play, but met-enkephalin (0.1–5 μg) and the enkephalinase inhibitor thiorphan (0.1–1 μg) were ineffective. DAMGO (0.1–10 ng) increased social play after infusion into both the shell and core subregions of the NAc. Last, intra-NAc infusion of CTAP (3 μg) prevented the development of social play-induced conditioned place preference. These findings identify NAc μ-opioid receptor stimulation as an important neural mechanism for the attribution of positive value to social interactions in adolescent rats. Altered NAc μ-opioid receptor function may underlie social impairments in psychiatric disorders such as autism, schizophrenia, or personality disorders.
Trait-Like Characteristics of the Sleep EEG across Adolescent Development
Authors: Tarokh Leila , Carskadon Mary A. , Achermann Peter
Published: April 2011

Waking and sleep data in adults show high heritability and trait-like characteristics in EEG spectra. This phenomenon has not been examined in children and adolescents where brain development influences the EEG. The present study examines whether a trait-like sleep EEG pattern is detectable across adolescent development. Two consecutive nights of standard sleep recordings were performed in 19 9–10-year-old children and 26 15–16-year-old teens, and were repeated 1.5–3 years later. EEG spectra averaged across the night for non-rapid eye movement and rapid eye movement sleep separately were classified using hierarchical cluster analysis, which showed that all 4 nights of a participant clustered together for a majority of participants. Intraclass correlation coefficients were also very high (>0.7) across nights separated by several years, indicating a trait-like feature of the sleep EEG. In summary, our results, using two measures of stability, indicate that a “trait-like” aspect can be detected in the sleep EEG across adolescent development despite considerable neurodevelopmental changes. This finding indicates that the brain oscillators responsible for generating the sleep EEG signal remain relatively stable across adolescent development.
Dissociable Effects of Lesions to Orbitofrontal Cortex Subregions on Impulsive Choice in the Rat
Authors: Mar Adam C. , Walker Alice L. J. , Theobald David E. , Eagle Dawn M. , Robbins Trevor W.
Published: April 2011

The orbitofrontal cortex (OFC) is implicated in a variety of adaptive decision-making processes. Human studies suggest that there is a functional dissociation between medial and lateral OFC (mOFC and lOFC, respectively) subregions when performing certain choice procedures. However, little work has examined the functional consequences of manipulations of OFC subregions on decision making in rodents. In the present experiments, impulsive choice was assessed by evaluating intolerance to delayed, but economically optimal, reward options using a delay-discounting paradigm. Following initial delay-discounting training, rats received bilateral neurotoxic or sham lesions targeting whole OFC (wOFC) or restricted to either mOFC or lOFC subregions. A transient flattening of delay-discounting curves was observed in wOFC-lesioned animals relative to shams—differences that disappeared with further training. Stable, dissociable effects were found when lesions were restricted to OFC subregions; mOFC-lesioned rats showed increased, whereas lOFC-lesioned rats showed decreased, preference for the larger-delayed reward relative to sham-controls—a pattern that remained significant during retraining after all delays were removed. When locations of levers leading to small–immediate versus large–delayed rewards were reversed, wOFC- and lOFC-lesioned rats showed retarded, whereas mOFC-lesioned rats showed accelerated, trajectories for reversal of lever preference. These results provide the first direct evidence for dissociable functional roles of the mOFC and lOFC for impulsive choice in rodents. The findings are consistent with recent human functional imaging studies and suggest that functions of mOFC and lOFC subregions may be evolutionarily conserved and contribute differentially to decision-making processes.
Stimulus Selectivity in Dorsal and Ventral Prefrontal Cortex after Training in Working Memory Tasks
Authors: Meyer Travis , Qi Xue-Lian , Stanford Terrence R. , Constantinidis Christos
Published: April 2011

The prefrontal cortex is known to represent different types of information in working memory. Contrasting theories propose that the dorsal and ventral regions of the lateral prefrontal cortex are innately specialized for the representation of spatial and nonspatial information, respectively (Goldman-Rakic, 1996), or that the two regions are shaped by the demands of cognitive tasks imposed on them (Miller, 2000). To resolve this issue, we recorded from neurons in the two regions, before and at multiple stages of training monkeys on visual working memory tasks. Before training, substantial functional differences were present between the two regions. Dorsal prefrontal cortex exhibited higher overall responsiveness to visual stimuli and higher selectivity for spatial information. After training, stimulus selectivity generally decreased, although dorsal prefrontal cortex retained higher spatial selectivity regardless of task performed. Ventral prefrontal cortex appeared to be affected to a greater extent by the nature of the task. Our results indicate that regional specialization for stimulus selectivity is present in the primate prefrontal cortex regardless of training. Dorsal areas of the prefrontal cortex are inherently organized to represent spatial information, and training has little influence on this spatial bias. Ventral areas are biased toward nonspatial information, although they are more influenced by training both in terms of activation and changes in stimulus selectivity.
Extended Play: Reflections of Heinrich Isaac's Music in Early Tudor England
Authors: Bryan John
Published: April 2011

The so-called Henry VIII's Book (London, British Library Add. MS 31922) contains two textless pieces by Isaac—his three-part Benedictus and the four-part La my—together with a number of other Franco-Flemish “songs without words” typical of the contents of manuscripts copied for the North Italian courts where the earliest viol consorts were being developed in the 1490s and early 1500s. Alongside these pieces are works by native English composers, including William Cornyshe, whose extended three-part Fa la sol has a number of stylistic traits in common with some works by Isaac (for example, his three-part Der Hundt) and Alexander Agricola (his three-part Cecus non judicat de coloribus) that were also transmitted in textless format. The fact that these latter two pieces were published in Hieronymus Formschneider's Trium vocum carmina (Nuremberg, 1538) while Cornyshe's Fa la sol was published in XX Songes (London, 1530) shows that this type of repertoire was still prized several years after the composers' deaths.

Analysis of musical connections between the work of Isaac and Cornyshe, as evident in pieces such as those from Henry VIII's Book—in particular, techniques employed by the composers to extend the structures of their “songs without words”—sheds fresh light on the reception in England of Isaac's music and that of his continental contemporary Agricola. Relevant considerations include the context in which these pieces were anthologized together and the introduction into England of viols similar to those Isaac may have known in Ferrara in 1502, when La my was composed. Such pieces are representative of a typical courtly repertoire that developed into the riches of the later Tudor instrumental consort music.
Isaac's Signature
Authors: Wegman Rob C.
Published: April 2011

The notion of the signature could serve as an appropriate metaphor by which to explore Heinrich Isaac as a man of his time and world. It may be mere coincidence that he has left more documents signed in his own hand than contemporary composers, but some of the documents he authenticated in this way really do attest to a new idea of professional musicianship that Isaac was the earliest and most successful in implementing: that of the professional composer who undertakes to produce new works under contractual obligation. Isaac is the first-known musician who signed a document specifically in this capacity. Yet his signature, or at least the assurance that he personally composed and signed a musical work, is also found in the context of practical musical sources, where they would appear to have no legal significance. Martin Just has shown, however, that the particular folios containing these compositions, in the manuscript Berlin 40021, were originally sent as letters. The implication is that Isaac's signature, in this case, is not an attribution so much as a mark of authentication—something that would have been required only if the musical works in question were sent, and changed hands, as part of a commercial transaction.

Taking the metaphor of the signature in a broader figurative sense, one could suggest that Isaac's work also bears his musical signature—namely in the personal style that his contemporaries tried to recognize and in some cases to characterize in words. Two authors who tried to capture the peculiar quality of Isaac's music are Paolo Cortesi and Heinrich Glarean. The latter's attempt is especially significant, since Glarean seems to attest to a new way of hearing and conceptualizing polyphony. Although it is hard to identify specifically which passages in Isaac's music he would have had in mind, the key to his appraisal seems to lie in a different way of conceptualizing the interplay of contrapuntal voices in contemporary music. To the extent that we can associate this with Isaac's musical signature, it would appear, once again, that this composer, more than any other, was at the forefront of some of the most significant developments in the music history of his time.
Airway Management Issues in the Intensive Care Unit: Revisiting Old Paradigms and Broadening Our Horizons
Authors: Keck Jeffrey P. , Mort Thomas C.
Published: April 2011

Airway management in the intensive care setting provides unique challenges that can be quite daunting, even for the most experienced practitioner. Airways are usually intubated for long periods, multiple comorbidities often interfere with "routine" airway management practices, and patients are often physiologically disadvantaged or hemodynamically unstable. Strapped with this calamity, the first responder to a patient with an acutely compromised airway is often someone less experienced with global airway management skills. As anesthesiologists, we are very familiar with the skill sets necessary to handle these predicaments, and as intensivists, we have the fortunate opportunity to share that wealth of information and experience. Airway care in the intensive care unit is a continuumfrom elective or emergent intubation, to airway preservation and hygiene, to elective or unintentional extubation. Thus, familiarization with the basics of airway management in routine and "first responder" settings should bolster confidence and greatly improve patient safety and outcomes.
Congenital Mitral Valve Regurgitation in Adult Patients
Authors: Jiang ShengLi , Gao ChangQing , Li BoJun , Ren ChongLei , Wang Yao , Zhang Tao , Xiao ChangSong , Wu Yang , Cheng TingTing , Zhang Lin
Published: April 2011

Objective: Congenital mitral valve regurgitation (MVR) is a rare disease found in adults. We report on our 5-year surgical experience with congenital MVR in adults.Methods: We reviewed the data for 48 consecutive patients (26 men), aged >18 years (median, 42 years; range, 18-78 years) who underwent operations for severe congenital MVR between June 2005 and May 2010. Patients with atrioventricular septal defect were excluded.Results: Congenital MVR was preoperatively diagnosed in 28 cases (58%). The lesions consisted of annular dilation (100%), valvular cleft (58%), prolapsed leaflet (40%), papillary muscle abnormality (5%), commissure fusion (2%), and leaflet deficiency (2%). Mitral valve repair was performed in 42 cases (88%) by means of Carpentier techniques. The other 6 patients underwent mitral valve replacement; one of these patients died of ventricular fibrillation 2 days after surgery. There were no other hospital deaths or late mortality. At the last follow-up (median, 38 months; range, 2-50 months), all 47 patients were in New York Heart Association functional class I or II. Echocardiography evaluations for the 42 patients who underwent the repairs revealed that 32 (76%) of the patients had no or trivial MVR and 10 patients (24%) had mild MVR. No patient underwent reoperation.Conclusion: Congenital MVR is rare and often misdiagnosed in adults. Mitral valve repair is feasible in the majority of patients, with excellent immediate and medium-term results.
Radicular dysfunction preponderance at early phase clinical evaluation in myelitis by Schistosoma mansoni
Authors: Vidal Claudio Henrique Fernandes , Silva Joacil Carlos da , Souza Jefferson Jane Oliveira , Bernardino Sara Pimentel Belleza , Ferreira Maria Lucia Brito
Published: April 2011

In neuroschistosomiasis, the spinal cord is the most common place of the disease. In high prevalent areas for schistosomiasis mansoni, the clinical alertness is important for an early diagnostic, in order to decrease the final neurological damage. This study provides some useful neurologic information about a series of patients with schistosomal myelitis. METHOD: The sample consisted of 13 schistosomiasis mansoni carriers examined at the moment of the diagnosis of myelitis. RESULTS: The classical triad (lumbago, weakness at the lower limbs and urinary dysfunctions) was documented in 11 (86.61%) patients. The distribution of the clinical forms was: myeloradicular in six patients (46.15%), radicular in four (30.76%) and myelitic in three (23.07%). CONCLUSION: The radicular dysfunction and their clinical associated forms were the most prominent pattern during the early phase of this disease.
Resposta do feijoeiro à adubação fosfatada em solos de cerrado com diferentes históricos de uso
Authors: Santos José Zilton Lopes , Furtini Neto Antônio Eduardo , Resende Álvaro Vilela de , Carneiro Leandro Flávio , Curi Nilton , Moretti Bruno da Silva
Published: April 2011

Os diferentes tempos de cultivo e as diferentes formas de manejo podem influenciar na disponibilidade dos nutrientes, entre os quais o P. Objetivou-se com este estudo avaliar a resposta do feijoeiro à adubação com diferentes doses de P, em quatro Latossolos de Cerrado sob diferentes históricos de uso. As amostras dos Latossolos - (LVd1) textura argilosa, (LVd2) textura média alta, (LVAd1) textura média e (LVAd2) textura média baixa - foram coletadas na camada de 0-20 cm de profundidade em locais cultivados há vários anos e, também, em áreas adjacentes não cultivadas (sob cerrado nativo). Com as amostras superficiais dos solos de cada área, foi conduzido um experimento em casa de vegetação, em delineamento inteiramente casualizado, num esquema fatorial 2 x 4 (dois históricos de uso cultivado e não cultivado, e quatro doses de P), com quatro repetições. As doses de P, equivalentes a 0, 120, 240 e 480 mg dm-3, foram aplicadas na forma de superfosfato triplo. A adição de doses crescentes de P aos solos promoveu aumento dos componentes relacionados ao crescimento do feijoeiro, principalmente matéria seca e conteúdo de P. A condição de solo já cultivado potencializou as respostas do feijoeiro à adubação, sobretudo na menor dose de P, e, nessa condição, houve maior absorção de P pelas plantas. O histórico de uso do solo influencia tanto a absorção quanto a eficiência de utilização de P pelas plantas; no entanto, as condições que promovem maior absorção são opostas àquelas que permitem maior eficiência de utilização do nutriente, sobretudo nas condições de solo já cultivado. A disponibilidade de P no solo foi mais dependente das características físicas, químicas e mineralógicas do que do histórico de uso do solo.
Educação e desafios da multiculturalização: uma pedagogia da sociedade civil
Authors: Barbosa Manuel
Published: April 2011

A progressiva complexificação cultural das sociedades contemporâneas, que se seguiu à extraordinária aceleração da mobilidade e da movimentação das pessoas através das fronteiras dos Estados, vem criando enormes desafios à coesão e à convivência no interior desses cenários e exige, em respeito aos princípios fundamentais da democracia e aos direitos humanos, respostas urgentes e adequadas. As entidades governamentais, por meio de políticas sociais e culturais de antidiscriminação e de redistribuição de bens e serviços, são necessárias ao desenho dessas respostas, porém, não são suficientes para induzir a estima social pelos estranhos culturais, em particular os recém-chegados aos países economicamente mais estáveis e avançados. O apreço pelos outros culturalmente diferentes depende essencialmente de uma mudança de atitudes e comportamentos e estes só podem evoluir para formas mais amigas das diferenças culturais com a contribuição da sociedade civil mediante uma pedagogia do exemplo, centrada em novos valores, novas atitudes, novos olhares e novos comportamentos. Este texto, a partir da análise e da reflexão sobre essa problemática, argumenta em favor do papel educativo da sociedade civil na organização da vida em comum entre estranhos culturais e propõe as linhas mestras da pedagogia que redefine a agenda educativa dessa sociedade em contextos multiculturais.
Seletividade de agrotóxicos utilizados em pomares de pêssego a adultos do predador Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae)
Authors: Castilhos Rodolfo Vargas , Grützmacher Anderson Dionei , Nava Dori Edson , Zotti Moisés João , Siqueira Paulo Ricardo Baier
Published: April 2011

A seletividade de dezesseis agrotóxicos utilizados na produção integrada e convencional de pêssego foi avaliada sobre a fase adulta de Chrysoperla externa através de bioensaios de exposição residual conduzidos em laboratório (temperatura de 25±1ºC, umidade relativa 70±10% e fotofase 14 horas), utilizando-se de metodologia prescrita pela "International Organization for Biological Control" (IOBC). Os agrotóxicos (% de ingrediente ativo na calda) azoxystrobina (0,016), captana (0,192), dodina (0,126), folpete (0,200), mancozebe (0,256), mancozebe + oxicloreto de cobre (0,140 + 0,096), tebuconazole (0,320), abamectina (0,002), óleo mineral 1 (2,420), óleo mineral 2 (1,920), dicloreto de paraquate (0,300) e glifosato (1,440) foram inócuos; deltametrina (0,002) foi levemente nocivo e dimetoato (0,160), fosmete (0,160) e malationa (0,240) foram nocivos a adultos de C. externa.
Influência da inversão do diâmetro veia porta/veia esplênica nos resultados do tratamento cirúrgico da hipertensão portal esquistossomótica
Authors: Ferraz Álvaro Antônio Bandeira , Campos Josemberg Marins , Araújo Júnior José Guido Corrêa de , Carvalho Márcio Rogério Carneiro de , Ribeiro Neto João Paulo , Ferraz Edmundo Machado
Published: April 2011

OBJETIVO: Avaliar a morbidade e a mortalidade no tratamento cirúrgico da hipertensão portal esquistossomótica em pacientes portadores de inversão do diâmetro entre a veia porta e veia esplênica. MÉTODOS: Estudo transversal retrospectivo, de pacientes submetidos ao tratamento cirúrgico da hipertensão no período entre setembro de 1993 e Janeiro de 2004. A população do estudo foi distribuída em dois grupos: a) Inversão - calibre da veia esplênica maior ou igual ao da veia porta) e b) grupo controle (calibre da veia porta maior que o da veia esplênica). Na análise estatística foram utilizados o teste t de student para diferença de médias, quiquadrado para diferença de proporções e o exato de Fisher para amostras reduzidas. RESULTADOS: 169 pacientes foram analisados com seguimento pós-operatório médio de 23,6 meses. 21 pacientes (12,4%) apresentavam a veia esplênica de igual ou maior calibre que a veia porta (Inversão - grupo de estudo). A média dos diâmetros pré-operatórios das veias porta e esplênica foram, respectivamente, 1,49/1,14cm no grupo controle, e 0,98/1,07cm no grupo de inversão. O diâmetro da veia porta foi significativamente maior no grupo controle quando comparado ao grupo de inversão (p<0,05). A presença de varizes de fundo gástrico foi identificada em 33,3% do grupo de inversão e em 38,5% dos pacientes do grupo controle. Recidiva hemorrágica pós-operatória ocorreu em 23,1% dos pacientes do grupo de inversão e em 13,4% no grupo controle (p>0,05). Na avaliação pós-operatória com ultrassonografia Doppler de vasos portais, não houve casos de trombose portal no grupo de inversão, e no grupo controle a trombose portal foi identificada em 16,9% dos pacientes (p<0,05). O óbito ocorreu em um (4,8%) paciente do grupo inversão, e a mortalidade foi de 4,1% no grupo controle (p>0,05). A média do nível sérico de plaquetas foi significativamente menor (65.950\mm□) no grupo de inversão do que no grupo controle (106.647\mm□) (p<0,05). CONCLUSÃO: Os resultados sugerem que a inversão do calibre veia porta\esplênica não representa uma contraindicação ao tratamento cirúrgico da hipertensão portal esquistossomótica.
Tumor de colisão de pele do pescoço
Authors: Chedid Helma Maria , Menezes Aglailton dos Santos , Aikawa Kiyoshi Fernandes , Lehn Carlos Neutzling , Rapoport Abrão , Mercante Ana Maria da Cunha , Curioni Otávio Alberto
Published: April 2011

O tumor de colisão ou tumor misto é uma neoplasia maligna de pele, relacionada à exposição solar e com índices de incidência de até 1,5%. Apresenta comportamento clínico peculiar, em relação às demais neoplasias malignas de pele e com diagnóstico histológico, caracterizado pela colisão entre um carcinoma basocelular e um carcinoma epidermóide, ou seja, duas neoplasias com histologias distintas e interface nítida entre ambas. O caso relatado foi de paciente do sexo masculino, 73 anos, com duas lesões cervicais de crescimento progressivo nos últimos meses. O tratamento realizado foi cirúrgico, com exame histológico demonstrando a presença de carcinoma de células escamosas contíguo ao carcinoma de células basais. O acometimento preferencial ocorre em homens de pele clara, na quinta ou sexta décadas de vida. Sua localização mais comum é na cabeça e pescoço, principalmente na parte central da face. O carcinoma basoescamoso é diagnóstico diferencial, definido através de critérios histológicos distintos, uma vez que ambas neoplasias apresentam comportamento clínico semelhante. Os índices de recidiva local variam de 12% a 45%, enquanto que é baixo na recidiva regional, de aproximadamente 7,5%. Os principais fatores prognósticos são o gênero do paciente, margens cirúrgicas, infiltração perineural e status linfonodal. O tratamento de escolha é a ressecção, sendo a radioterapia indicada na sua adjuvância e lesões irressecáveis. A recidiva local é o principal fator limitante na sobrevida livre de doença que apresenta resultados pobres.
A aula e a sala de aula: um espaço-tempo de produção de conhecimento
Authors: Ribeiro Victoria Maria Brant , Ribeiro Adriana Maria Brant
Published: April 2011

Considera-se que a relação estabelecida entre professor e aluno é cultural, mas é também pedagógica, porque o espaço de convívio é mediado por conhecimentos que são, intencionalmente, ensinados e aprendidos. Nesse quadro, compreende-se que o exercício da docência deve, necessariamente, incluir momentos nos quais sejam feitas reflexão e crítica sobre as formas e o significado de ensinar, de trazer o outro para a sua perspectiva e com ele também aprender, momentos de experimentar situações desse acontecimento "feiticeiro" que se chama "sala de aula". São apresentados métodos e técnicas que possibilitam transformar estes momentos em oportunidades para construir conhecimento.
Fratura de patela com ruptura do tendão patelar em um cão: relato de caso
Authors: Filgueira F.G.F , Azevedo A. S , Fernandes T.H.T , Nunes G.D.L , Palmeira R.B , Araújo B.M , Carneiro R.S , Nóbrega Neto P.I , Sá M.J.C
Published: April 2011

Relatou-se a ocorrência de fratura patelar associada à ruptura do tendão patelar em um cão sem raça definida, macho, atendido em um hospital veterinário escola. A técnica utilizada foi a combinação de fixação óssea com fio de Kirschner e aplicação de banda de tensão, recomendada em fraturas transversais da patela. Para reparar a lesão do tendão patelar, realizou-se a sutura tipo Kessler modificada com fio de náilon 0,80mm. No retorno do paciente, 150 dias após o procedimento cirúrgico, havia processo cicatricial ósseo avançado da patela. Concluiu-se que as medidas adotadas para reparação da lesão foram eficazes