bluuebunny/crossref_metadata_2025 huggingface dataset:
Neural responses to sensory novelty with and without conscious access
Authors: Osorio Sergio , Irani Martín , Herrada Javiera , Aboitiz Francisco
Published: November 2020

Detection of novel stimuli that violate statistical regularities in the sensory scene is of paramount importance for the survival of biological organisms. Event-related potentials, phasic increases in pupil size, and evoked changes in oscillatory power in the theta (4-8 Hz) frequency range have been proposed as markers of sensory novelty detection. However, how conscious access to novelty modulates these different brain responses is not well understood. Here, we studied the neural responses to sensory novelty in the auditory modality with and without conscious access. We identified individual thresholds for conscious auditory discrimination and presented to our participants sequences of tones, where the last stimulus could be another standard, a subthreshold target or a suprathreshold target. Participants were instructed to report whether the last tone of each sequence was the same or different from those preceding it. Results indicate that stimulus evaluation and overt decision-making mechanisms, indexed by the P3 event-related response and reaction times, best predict whether a novel stimulus will be consciously accessed or not. In contrast, increased pupil size does not predict conscious access to novelty but reflects unexpected sensory uncertainty. These results highlight the interplay between bottom-up and top-down mechanisms and how the brain weights neural responses to novelty and uncertainty during perception and goal-directed behavior.
CryoET structures of immature HIV Gag reveal a complete six-helix bundle and stabilizing small molecules distinct from IP6
Authors: Mendonça Luiza , Sun Dapeng , Ning Jiying , Liu Jiwei , Kotecha Abhay , Olek Mateusz , Frosio Thomas , Fu Xiaofeng , Himes Benjamin A , Kleinpeter Alex B. , Freed Eric O. , Zhou Jing , Aiken Christopher , Zhang Peijun
Published: November 2020

Gag is the major HIV-1 structural polyprotein precursor. The Gag SP1 domain with the last residues of CA have been hypothesized to form a six-helix bundle necessary for particle assembly, but this bundle has not been fully resolved. Here, we determined the structures of complete CA-SP1 six-helix bundle connecting to the NC domain, from bothin vitroGag assemblies and viral-like particles (VLPs) carrying a T8I mutation in SP1, to near-atomic resolutions using cryoET and subtomogram averaging. The structures revealed novel densities, however distinct from IP6, inside the six-helix bundle of Gag assemblies, stabilizing the immature lattice. Interestingly, the T8I mutation impaired proteolytic cleavage of Gag at both SP1 boundaries. Our findings signify the involvement of small molecules in immature Gag assembly and provide the structural basis for development of small molecule inhibitors that stabilize SP1 helix, thus interfere with PR-mediated virus maturation.
Nav1.3 and fibroblast growth factor homologous factor 14 are primary determinants of the TTX-sensitive sodium current in mouse adrenal chromaffin cells
Authors: Martinez-Espinosa P.L. , Yang C. , Xia X.M. , Lingle C.J.
Published: November 2020

Adrenal chromaffin cells (CCs) in rodents express a rapidly inactivating, TTX-sensitive sodium current. The current has generally been attributed to Nav1.7, although a possible role for Nav1.3 has also been suggested. Nav channels in rat CCs rapidly inactivate into two separable pathways, which differ in their time course of recovery from inactivation. One population recovers with time constants similar to traditional fast inactivation and the other about 10-fold slower. Inactivation properties suggest that the two pathways result from a single homogenous population of channels. Here we probe the properties and molecular components of the Nav current present in mouse CCs. We first confirm that functional properties of Nav current in rat and mouse cells are generally similar in terms of activation range, steady-state inactivation, and dual pathway fast inactivation. The results then show that all inward Nav current is absent in CCs from Nav1.3 KO mice. Subsequently, in a mouse with KO of fibroblast growth factor homology factor 14 (FGF14), we find that the slow component of recovery from fast inactivation is completely absent in most CCs, with no change in the time constant of fast recovery. Experiments probing the use-dependence of Nav current diminution between WT and FGF14 KO mice directly demonstrate a role of slow recovery from inactivation in determination of Nav current availability. Overall, the results indicate that the FGF14-mediated inactivation is the major determinant in defining use-dependent changes in Nav availability in CCs. We also consider the potential impact that inactivating FGF’s with different recovery kinetics can exert on differential use-dependent changes in Nav availability.
MINSTED fluorescence localization and nanoscopy
Authors: Weber Michael , Leutenegger Marcel , Stoldt Stefan , Jakobs Stefan , Mihaila Tiberiu S. , Butkevich Alexey N. , Hell Stefan W.
Published: November 2020

We introduce MINSTED, a stimulated-emission-depletion (STED) based fluorescence localization and super-resolution microscopy concept providing spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED donut, and hence the point of minimal STED, serves as a movable reference coordinate for fluorophore localization. As the STED rate, the background, and the required number of fluorescence detections are low compared to most other STED microscopy and localization methods, MINSTED entails substantially less fluorophore bleaching. In our implementation, 200-1000 detections per fluorophore provide a localization precision of 1-3 nm in standard deviation, which in conjunction with independent single fluorophore switching translates to a ~100-fold improvement of far-field microscopy resolution over the diffraction limit. The performance of MINSTED nanoscopy is demonstrated by imaging the distribution of Mic60 proteins in the mitochondrial inner membrane of human cells.
Catestatin induces glycogenesis by stimulating phosphoinositide 3-kinase-AKT pathway
Authors: Bandyopadhyay Gautam , Tang Kechun , Webster Nicholas J.G. , van den Bogaart Geert , Mahata Sushil K.
Published: November 2020

Aim

Defects in hepatic glycogen synthesis contribute to postprandial hyperglycemia in type 2 diabetic (T2D) patients. Chromogranin A (CgA) peptide Catestatin (CST: hCgA352-372) has been shown to improve glucose tolerance in insulin-resistant mice. Here, we seek to determine whether CST also reduces hyperglycemia by increasing hepatic glycogen synthesis.

Methods

We determined liver glycogen, glucose-6-phosphate (G6P), uridine diphosphate glucose (UDPG), and glycogen synthase (GYS2) activities; plasma insulin, glucagon, norepinephrine (NE), and epinephrine (EPI) levels in fed and fasted liver of lean and obese mice as well as in CST knockout (CST-KO) mice after treatments with saline, CST, or insulin. We also determined glycogen synthesis and glycogenolysis in primary hepatocytes. In addition, we analyzed phosphorylation signals of Insulin receptor (IR), insulin receptor substrate-1 (IRS-1), phosphatidylinositol dependent kinase-1 (PDK-1), GYS2, glycogen synthase kinase-3β (GSK-3β), AKT (an enzyme in AKR mouse that produces Thymoma)/PKB (protein kinase B) and mTOR (mammalian/mechanistic target of rapamycin) by immunoblotting.

Results

CST stimulated glycogen accumulation in fed and fasted liver and in primary hepatocytes. CST reduced plasma NE and EPI levels, suggesting that CST promotes glycogenesis by inhibiting catecholamine-induced glycogenolysis. CST also directly stimulated glycogenesis and inhibited NE and EPI-induced glycogenolysis in hepatocytes. CST elevated the levels of UDPG and increased GYS2 activity, thus redirecting G6P to the glycogenic pathway. CST-KO mice had decreased liver glycogen that was restored by treatment with CST, reinforcing the crucial role of CST in hepatic glycogenesis. CST can improve insulin signals downstream of insulin receptor IR and IRS-1 by enhancing phospho-AKT signals through stimulation of PDK-1 and mTORC2 (mTOR complex 2) activities.

Conclusions

We conclude that CST directly promotes the glycogenic pathway and reduces plasma glucose levels in insulin-resistant mice by (i) reducing glucose production, (ii) increasing glycogen synthesis from UDPG, and (iii) reducing glycogenolysis. This is achieved by enhancing downstream insulin signaling.
Heparin MicroIslands to Promote Enhanced Diabetic Wound Healing Outcomes
Authors: Pruett Lauren , Jenkins Christian , Singh Neharika , Catallo Katarina , Griffin Donald
Published: November 2020

A powerful tool to improve tissue integration with biomaterial scaffolds for the regeneration of damaged tissues is to promote cell migration using chemotactic gradients of growth factors. This approach has been realized by the exogenous delivery of growth factors, which unfortunately also limits the scaffold’s ability to meet each wound’s unique spatial and temporal regenerative needs. To address this limitation, we present a new approach to gradient generation by incorporating heparin microislands, which are spatially isolated heparin-containing microparticles that create chemotactic microgradients through reorganization of endogenous local growth factors. We incorporated heparin microislands within microporous annealed particle (MAP) scaffolds, which allows us to tune their incorporation ratiometrically to create a heterogenous microenvironment. In this manuscript, we demonstrate the ability of heparin microislands to organize uniform growth factors into spontaneous microgradients and control downstream cell migrationin vitro. Further, we present their ability to significantly improve wound healing outcomes (epidermal regeneration and vascularization) in a diabetic wound model relative to two clinically relevant controls.
Impaired light adaptation of ON-sustained ganglion cells in early diabetes is attributable to diminished dopamine D4 receptor sensitivity
Authors: Flood Michael D. , Wellington Andrea J. , Eggers Erika D.
Published: November 2020

Purpose: It has been known for some time that normal retinal signaling is disrupted early on in diabetes, before the onset of the vascular pathologies associated with diabetic retinopathy. There is growing evidence that levels of retinal dopamine, a neuromodulator that mediates light adaptation, may also be reduced in early diabetes. Previously, we have shown that after six weeks of diabetes in a mouse model, light adaptation is impaired at the level of ON-sustained (ON-s) ganglion cells. The purpose of this study was to determine whether changes in dopamine receptor sensitivity contribute to this dysfunction. Here we used single cell retinal patch-clamp recordings from the mouse retina to determine how activating dopamine type D4 receptors (D4Rs) changes the light-evoked and spontaneous excitatory inputs to ON-s ganglion cells, in both control and diabetic animals. We also used in-situ fluorescent hybridization to assess whether D4R expression was impacted by diabetes. We found that D4R activation had a smaller impact on light-evoked excitatory inputs to ON-s ganglion cells in diabetic retinas compared to controls. This impaired D4R signaling is not attributable to a decline in D4R expression, as we found increased D4R mRNA density in the outer plexiform layer in diabetic retinas. This suggests that the cellular machinery of dopaminergic signaling is itself disrupted in early diabetes and may be amenable to chronic dopamine supplementation therapy.
The genetic and ecological landscape of plasmids in the human gut
Authors: Yu Michael K. , Fogarty Emily C. , Eren A. Murat
Published: November 2020

Despite their prevalence and impact on microbial lifestyles, ecological and evolutionary insights into naturally occurring plasmids are far from complete. Here we developed a machine learning model, PlasX, which identified 68,350 non-redundant plasmids across human gut metagenomes, and we organized them into 1,169 evolutionarily cohesive ‘plasmid systems’ using our sequence containment-aware network partitioning algorithm, MobMess. Similar to microbial taxa, individuals from the same country tend to cluster together based on their plasmid diversity. However, we found no correlation between plasmid diversity and bacterial taxonomy. Individual plasmids were often country-specific, yet most plasmid systems spanned across geographically distinct human populations, revealing cargo genes that likely respond to environmental selection. Our study introduces powerful tools to recognize and organize plasmids, uncovers their tremendous diversity and intricate ecological and evolutionary patterns in naturally occurring habitats, and demonstrates that plasmids represent a dimension of ecosystems that is not explained by microbial taxonomy alone.
Entrepreneurship education intention and entrepreneurial intention amongst disadvantaged students: an empirical study
Authors: Shrivastava Umesh , Acharya Satya Ranjan
Published: November 2020

Purpose

Disadvantaged students face social exclusion and undergo a different treatment than mainstream students. This alters their entrepreneurial intention subsequently. This study aims to investigate the factors affecting disadvantaged students’ intention in their willingness to undergo entrepreneurship education as a vocational course. The variables include self-efficacy, need for achievement (nAch) and family background. The paper further examines whether entrepreneurship education intention enhances their entrepreneurial intention.

Design/methodology/approach

This study uses a deductive quantitative study as the chosen approach as it ensures complete anonymity and hence researcher bias is minimized. The sample consists of the third year, final year and postgraduate first year disadvantaged students from different streams of engineering, economics, arts and commerce. The study was conducted with a total of 319 students completing the questionnaire which used a five-point Likert scale.

Findings

Using the theory of planned behavior (TPB), the results show that willingness of disadvantaged students to study entrepreneurship as a vocational course is highly driven by their family background followed by self-efficacy and nAch. The results further strengthen the TPB and has implications for educators of entrepreneurship and a possibility of a widening of entrepreneurship education in disadvantaged community.

Research limitations/implications

The study measured attitudes and willingness with intentions, but not actual behavior as this was a cross-sectional study. Also, repeated observations could not be made and dynamics of change could not be captured.

Originality/value

This is one of the few studies focused on entrepreneurial intention of students who are socially excluded and therefore it offers a possibility of widening of entrepreneurship education in countries such as India which display a collectivist culture and provides an intention-based linkage to entrepreneurship education among disadvantaged students. This study also puts subjective norm as a strong predictor of intentions which previous studies have refuted. The findings also suggest that there is a strong intent to study entrepreneurship among disadvantaged students in India, which makes entrepreneurship education a seemingly acceptable choice of education and suggests promise for its wider reach and penetration.
The effects of Tier-1 capital to risk management and profitability on performance using multiple fixed effect panel data model
Authors: Rangkuti Zulkifli
Published: November 2020

Purpose

This paper aims to examine the effects of Tier-1 capital toward risk management and profitability on the performance of Indonesian Commercial Banks.

Design/methodology/approach

The research population consisted of all commercial banks listed on the Indonesia Stock Exchange. The data were in the form of financial statements of commercial banks for the periods of 2012 to 2016 with a total of 42 companies (bank). From a total of 42 commercial banks listed in the Indonesia Stock Exchange, not all of them met the criteria. Commercial banks that meet these criteria are as many as 28 banks are sampled research.

Findings

Tier-1 capital has a positive direct effect on risk management, Tier-1 capital has a positive indirect effect on profitability with risk management as a mediation variable, risk management has a positive direct effect on profitability, Tier-1 capital has a positive indirect effect on performance with risk management and profitability as mediation variables, risk management has a positive indirect effect on performance with as mediation variable and profitability has a positive impact on performance.

Originality/value

The originality of this research can be seen from the causal relationship between the effects of Tier-1 capital, risk management and profitability on the performance of commercial banks in the context of stock performance among Indonesia commercial banks. Also, the analysis tools using multiple fixed effect panel data models in this research as a novelty in this research. In addition, previous research findings remain inconsistent with one another. By conducting this research, it is expected that more consistent research findings than the previous ones can be generated. Sluggish global economic conditions, which result in declined bank performance are an interesting topic to investigate. The paper uses an original sample, 28 Indonesian banks in 2012-2016. Also, it links Tier 1 capital with risk management and performance in a novel theoretical framework.
An environmental approach to police misconduct: exploring situational prevention possibilities to understanding and preventing police misconduct
Authors: Hine Kelly Amy , Porter Louise E. , Ransley Janet
Published: November 2020

Purpose

This paper explores the applicability of environmental theories to understanding patterns of police misconduct. In turn, it aims to offer a method for identifying prevention techniques that can be practically applied by policing agencies.

Design/methodology/approach

The study empirically examined 84 substantiated matters of police misconduct in Queensland, Australia. The matters were content-analysed for elements of the first level of the crime triangle. These elements were then analysed to identify their relationships with the situational precipitators that initiated the misconduct; proactive misconduct and situational misconduct.

Findings

The two types of initiating misconduct had differing relationships with the crime triangle elements. Therefore, specific prevention techniques can be tailored by policing agencies to address and prevent each type of misconduct more successfully. The paper discusses these findings in terms of preventative measures according to the second preventative level of the crime triangle and situational crime prevention techniques.

Originality/value

This paper provides an alternative approach to understanding and preventing police misconduct by exploring the applicability of environmental theories. It finds that environmental theories offer a feasible approach for policing agencies to understand and tailor prevention of police misconduct in their jurisdictions.
Increased acclimation ability accompanies a thermal niche shift of a recent invasion
Authors: Bujan Jelena , Charavel Ellouène , Bates Olivia K. , Gippet Jérôme M. W. , Darras Hugo , Lebas Claude , Bertelsmeier Cleo
Published: November 2020

Globalization is removing dispersal barriers for the establishment of invasive species and enabling their spread to novel climates. New thermal environments in the invaded range will be particularly challenging for ectotherms, as their metabolism directly depends on environmental temperature. However, we know little about the role climatic niche shifts play in the invasion process, and the underlining physiological mechanisms.

We tested if a thermal niche shift accompanies an invasion, and if native and introduced populations differ in their ability to acclimate thermal limits.

We used an alien ant species—Tapinoma magnum—which recently started to spread across Europe. Using occurrence data and accompanying climatic variables, we measured the amount of overlap between thermal niches in the native and invaded range. We then experimentally tested the acclimation ability in native and introduced populations by incubating T. magnum at 18, 25 and 30°C. We measured upper and lower critical thermal limits after 7 and 21 days.

We found that T. magnum occupies a distinct thermal niche in its introduced range, which is on average 3.5°C colder than its native range. Critical thermal minimum did not differ between populations from the two ranges when colonies were maintained at 25 or 30°C, but did differ after colony acclimation at a lower temperature. We found twofold greater acclimation ability of introduced populations to lower temperatures, after prolonged incubation at 18°C.

Increased acclimation ability of lower thermal limits could explain the expansion of the realized thermal niche in the invaded range, and likely contributed to the spread of this species to cooler climates. Such thermal plasticity could be an important, yet so far understudied, factor underlying the expansion of invasive insects into novel climates.
Manipulating plant community composition to steer efficient N‐cycling in intensively managed grasslands
Authors: Abalos Diego , De Deyn Gerlinde B. , Philippot Laurent , Oram Natalie J. , Oudová Barbora , Pantelis Ioannis , Clark Callum , Fiorini Andrea , Bru David , Mariscal‐Sancho Ignacio , van Groenigen Jan Willem
Published: November 2020

Minimizing nitrogen (N) losses and increasing plant N uptake in agroecosystems is a major global challenge. Ecological concepts from (semi)natural grasslands suggest that manipulating plant community composition using plant species with different traits may represent a promising opportunity to face this challenge. Here, we translate these trait‐based concepts to agricultural systems in a field experiment, aiming to reveal the main determinants of how plant community composition regulates N‐cycling in intensively managed grasslands.

We focused on key N pools (plant N from soil and from biological N‐fixation, soil mineral N and N2O emissions) as well as on biological drivers of N‐cycling in soil (abundance of N‐cycling microbial communities, earthworm populations and arbuscular mycorrhizal fungi), using three common grass and one legume species in monoculture, two‐ and four‐species mixtures. We hypothesized that: (a) plant species mixtures increase plant N uptake, reduce soil mineral N concentrations and N2O emissions and promote the abundance of biological N‐cyclers; (b) legume presence stimulates N pools, fluxes and biological N‐cycling activity, (c) but in combination with a grass with acquisitive traits, more N is retained in the plant community, while N2O emissions are reduced.

We found that mixtures increased plant N and lowered the soil mineral N pool compared to monocultures. However, plant species identity played an overarching role: Legume presence increased N2O emissions, plant N pools, soil mineral N and the abundance of N‐cycling microbes and earthworms. Combining the legume with a grass with low leaf dry matter content and high root length density (and with high root biomass) reduced the higher soil mineral N and N2O emissions induced by the legume, while harnessing positive effects on plant N pools and biological N‐fixation.

Synthesis and applications. Our results show the potential of plant community composition to steer N‐cycling in fertilized agroecosystems, paving the way for a more biologically based agriculture. Legumes will play a crucial role, but selecting an optimum companion species is key for the sustainability of the agroecosystem.
An integrated population model for estimating the relative effects of natural and anthropogenic factors on a threatened population of steelhead trout
Authors: Scheuerell Mark D. , Ruff Casey P. , Anderson Joseph H. , Beamer Eric M.
Published: November 2020

Assessing the degree to which at‐risk species are regulated by density‐dependent versus density‐independent factors is often complicated by incomplete or biased information. If not addressed in an appropriate manner, errors in the data can affect estimates of population demographics, which may obfuscate the anticipated response of the population to a specific action.

We developed a Bayesian integrated population model that accounts explicitly for interannual variability in the number of reproducing adults and their age structure, harvest and environmental conditions. We apply the model to 41 years of data for a population of threatened steelhead trout Oncorhynchus mykiss using freshwater flows, ocean indices and releases of hatchery‐born conspecifics as covariates.

We found compelling evidence that the population is under density‐dependent regulation, despite being well below its historical population size. In the freshwater portion of the lifecycle, we found a negative relationship between productivity (offspring per parent) and peak winter flows, and a positive relationship with summer flows. We also found a negative relationship between productivity and releases of hatchery conspecifics. In the marine portion of the lifecycle, we found a positive correlation between productivity and the North Pacific Gyre Oscillation.

Synthesis and applications. The evidence for density‐dependent population regulation, combined with the substantial loss of juvenile rearing habitat in this river basin, suggests that habitat restoration could benefit this population of at‐risk steelhead. Our results also imply that hatchery programmes for steelhead need to be considered carefully with respect to habitat availability and recovery goals for wild steelhead. If releases of hatchery steelhead have indeed limited the production potential of wild steelhead, there are likely significant trade‐offs between providing harvest opportunities via hatchery steelhead production and achieving wild steelhead recovery goals. Furthermore, harvest rates on wild fish have been sufficiently low to ensure very little risk of overfishing.
The value of time‐series data for conservation planning
Authors: García‐Barón Isabel , Giakoumi Sylvaine , Santos María Begoña , Granado Igor , Louzao Maite
Published: November 2020

Protected areas (PAs) are increasingly being used world‐wide for the conservation and management of wildlife. Systematic conservation planning (SCP) aims at ensuring biodiversity persistence while minimizing the threats faced by the species and/or the economic costs related to protection. To account for spatio‐temporal interactions between species and human threats, conservation planning for mobile wildlife requires time‐series data derived from monitoring of species and human threats, a process that is costly and technically challenging. Therefore, assessments of the monitoring period needed to ensure sufficient data input in the design of efficient, adequate and representative networks of PAs are crucial.

We demonstrated the value of time‐series data in conservation planning by implementing SCP and data from different monitoring periods to identify priority conservation areas for highly mobile marine megafauna accounting for their main threat: commercial fishing. Two analyses of 10 reserve‐design scenarios each, replicated as many times as the data composing each scenario permitted were run in Marxan. The best solutions of the planning scenarios were statistically compared using the Cohen's Kappa test. We also assessed differences in spatial similarity among and within scenarios using the Wilcoxon nonparametric test and a non‐metric multidimensional scaling analysis. Finally, we compared the necessary cost and the area selected for each scenario.

Our study highlights the importance of time‐series ecological and socioeconomic data for the robust selection of priority conservation areas. The results revealed different thresholds of the minimum temporal data required to design efficient networks of PAs for highly mobile species, demonstrating that the incorporation of data covering longer periods to the scenarios produce a more robust selection of priority conservation areas. Conservation plans using data covering <3 years were missing important priority areas.

Synthesis and applications. We provide a method for estimating the minimum number of years of monitoring required to design efficient networks of protected areas that ensure the persistence of highly mobile species such as cetaceans and seabirds. This method can be used within an adaptive management framework to evaluate whether a network of protected areas performs as planned, and to test whether management strategies should be altered or adjusted in response to local and global changes.
Trade‐offs in above‐ and below‐ground biomass allocation influencing seedling growth in a tropical forest
Authors: Umaña María Natalia , Cao Min , Lin Luxiang , Swenson Nathan G. , Zhang Caicai
Published: November 2020

Plants allocate biomass to different organs in response to resource variation for maximizing performance, yet we lack a framework that adequately integrates plant responses to the simultaneous variation in above‐ and below‐ground resources. Although traditionally, the optimal partition theory (OPT) has explained patterns of biomass allocation in response to a single limiting resource, it is well‐known that in natural communities multiple resources limit growth. We study trade‐offs involved in plant biomass allocation patterns and their effects on plant growth under variable below‐ and above‐ground resources—light, soil N and P—for seedling communities.

We collected information on leaf, stem and root mass fractions for more than 1,900 seedlings of 97 species paired with growth data and local‐scale variation in abiotic resources from a tropical forest in China.

We identified two trade‐off axes that define the mass allocation strategies for seedlings—allocation to photosynthetic versus non‐photosynthetic tissues and allocation to roots over stems—that responded to the variation in soil P and N and light. Yet, the allocation patterns did not always follow predictions of OPT in which plants should allocate biomass to the organ that acquires the most limiting resource. Limited soil N resulted in high allocation to leaves at the expense of non‐photosynthetic tissues, while the opposite trend was found in response to limited soil P. Also, co‐limitation in above‐ and below‐ground resources (light and soil P) led to mass allocation to stems at the expense of roots. Finally, we found that growth increased under high‐light availability and soil P for seedlings that invested more in photosynthetic over non‐photosynthetic tissues or/and that allocated mass to roots at the expense of stem.

Synthesis. Biomass allocation patterns to above‐ and below‐ground tissues are described by two independent trade‐offs that allow plants to have divergent allocation strategies (e.g. high root allocation at the expense of stem or high leaf allocation at the expense of allocation to non‐photosynthetic tissues) and enhance growth under different limiting resources. Identifying the trade‐offs driving biomass allocation is important to disentangle plant responses to the simultaneous variation in resources in diverse forest communities.
Large mammalian herbivores contribute to conspecific negative density dependence in a temperate forest
Authors: Murphy Stephen J. , Comita Liza S.
Published: November 2020

The Janzen–Connell Hypothesis (JCH) predicts that density‐responsive and host‐specific natural enemies limit the population sizes of abundant species. Importantly, these interactions help to maintain local community diversity through time. While ample evidence exists for the demographic predictions of the JCH, it remains unclear which natural enemies drive these dynamics across different plant communities.

While large mammalian herbivores are often assumed to lack the specialized diet needed to drive Janzen–Connell effects, they do show a degree of host‐preference that could drive density‐dependent plant demography. However, the potential role of large mammalian herbivores in Janzen–Connell interactions has only rarely been investigated.

Using 204 seedling transects (1 m × 10 m) at 51 sites across a 900‐ha forested reserve in southwestern Pennsylvania (USA), we examined the role that large mammals play in driving conspecific negative density dependence (CNDD) in temperate tree seedlings. Individual fences were erected around half of the transects (n = 102) to exclude large mammals, and were paired with adjacent unfenced transects. Within transects, a total of ~15,000 individual seedlings were monitored over three growing seasons.

Demographic neighbourhood models were constructed to examine the influence of neighbourhood composition and density on seedling survival and growth. An interaction term between conspecific neighbour density and fencing treatment was included to test the hypothesis that large herbivores cause CNDD.

We found that seedling survival was influenced by both conspecific neighbour density and fencing. CNDD was strongest when large mammals were allowed access to seedlings, and these results were driven by two abundant taxa (Prunus serotina and Fraxinus spp). Despite evidence that large mammals mediate CNDD, we found no effect of fencing on rarified species richness or evenness in seedling transects during the study.

Synthesis. Understanding the specific natural enemies driving conspecific negative density dependence remains vital for understanding the maintenance of forest diversity across the globe. Our results indicate that large mammalian herbivores are capable of driving CNDD in temperate tree species. These results suggest that large mammals may be an important and generally overlooked agent contributing to Janzen–Connell interactions in forest communities. We expect that further research examining large mammals in other systems will be important in the future.
Functional diversity of decomposers modulates litter decomposition affected by plant invasion along a climate gradient
Authors: Luan Junwei , Liu Shirong , Li Siyu , Whalen Joann K. , Wang Yi , Wang Jingxin , Liu Yanchun , Dong Wei , Chang Scott X.
Published: November 2020

Litter decomposition is fundamental to carbon (C) and nutrient cycling in ecosystems, which could be altered by plant invasion. The impacts of plant invasion on litter decomposition are generally predicted by trait difference between leaf litters of invasive and non‐invasive species. However, plant invasion not only changes litter composition, but might also increase the activity or change the functional diversity of decomposers to alter litter decomposition, which is barely studied, and the effect could be different under varied climate conditions.

We studied decomposition of litters from non‐invasive and invasive native plants, as affected by litter treatments (in a mixture or alone) and decomposer organisms of different functional groups (by controlling the mesh size of litterbags), in sites with or without an invasive woody grass, Moso bamboo Phyllostachys edulis, at seven locations across a climate gradient.

We show that greater decomposer functional diversity, particularly the presence of macrofauna, accelerated the cycling of litter C and nitrogen (N), increased the climatic sensitivities of decomposition rates, but decreased the N use efficiency of decomposers (represented by litter C to N loss ratio). Litter decomposed in mixtures decomposed faster (by 9.5%) and had more N loss (by 28.9%) than that of the monoculture, regardless of the functional diversity of decomposers. In contrast, the invasion of Moso bamboo slowed decomposition and decreased N use efficiency; this negative effect could be reversed when macrofauna were excluded from the decomposition process, which challenges the nutrient facilitation hypothesis. Bamboo invasion depressed the climatic sensitivity of decomposer functional groups when macrofauna were present but not when macrofauna were excluded.

Synthesis. We found that the functional diversity of decomposer organisms modulates and largely determines litter decomposition affected by a woody grass invasion along a climate gradient. These results suggest that, under current and future climate, including the changes in decomposer functional groups, particularly macrofauna, and their interaction with litter traits, would provide a mechanistic and more reliable prediction on ecosystem functions altered by invaders than a functional trait‐based framework.
Safety First: Expanding the Global Financial Safety Net in Response to COVID‐19
Authors: Gallagher Kevin P. , Gao Haihong , Kring William N. , Ocampo José A. , Volz Ulrich
Published: November 2020

We call for strengthening the Global Financial Safety Net (GFSN) to manage the economic effects of COVID‐19, in particular the massive capital outflows from emerging market and developing economies EMDEs and the global shortage of dollar liquidity. Both the United Nations (UN) and the International Monetary Fund (IMF) estimate that EMDEs need an immediate $2.5 trillion, yet the financing available to them is just $700 to $971 billion. To meet these immediate needs we propose to: (1) broaden the coverage of the Federal Reserve currency swaps; (2) issue at least $500 billion of special drawing rights through the IMF; (3) improve the IMF’s precautionary and emergency facilities; (4) establish a multilateral swap facility at the IMF; (5) increase the resources and geographic coverage of regional financial arrangements; (6) coordinate capital flow management measures; (7) initiate debt restructuring and relief initiatives; and (8) request that credit‐rating agencies stop making downgrades during the emergency. It argues that leaders should swiftly move to address these structural gaps in the GFSN: (1) agree on a quota reform at the IMF; (2) create an appropriate sovereign debt restructuring regime; (3) expand surveillance activity; and (4) adopt IMF governance reform and strengthen its relations with all agents of the GFSN. All of these reforms must be calibrated toward a just transition to a more stable, inclusive, and sustainable global economy.
Antiviral prophylaxis for hepatitis B carriers improves the prognosis of diffuse large B‐cell lymphoma in Taiwan – a population‐based study
Authors: Huang Huai‐Hsuan , Hsiao Fei‐Yuan , Chen Ho‐Min , Wang Chen‐Yu , Ko Bor‐Sheng
Published: November 2020

The prevention of hepatitis B virus (HBV) reactivation during rituximab treatment for diffuse large B‐cell lymphoma (DLBCL) is important in the HBV‐endemic area. This population‐based study examines the impact of antiviral prophylaxis for DLBCL patients with HBV infections. We identified 3702 adult patients with newly diagnosed DLBCL between 2011 and 2015 receiving R‐CHOP, R‐CVP, CHOP or CVP from the Taiwan Cancer Registry. We further stratified them into three groups: HBsAg‐negative patients (HBV‐negative, N = 2921), HBV carriers who received antiviral prophylaxis (HBV + Px, N = 711), and HBV carriers who did not receive antiviral prophylaxis (HBV + No Px, N = 70). HBV + Px patients were the youngest, and 69·4% received entecavir for antiviral prophylaxis. The median overall survival (mOS) of HBV‐negative and HBV + Px patients was similar (74·23 months and not reached, respectively). However, the mOS of HBV + No Px patients was only 35·61 months (P = 0·0028 compared with HBV + Px patients), indicating that antiviral prophylaxis improves OS in HBsAg‐positive DLBCL patients. The multivariate analysis showed that the HBV status and antiviral prophylaxis was an independent prognostic factor. In conclusion, our population‐based study illustrates the importance of antiviral prophylaxis in HBsAg‐positive DLBCL patients. Under antiviral prophylaxis, the survival of DLBCL patients with HBV infections was comparable to that of HBV‐negative patients.
An introduction to the economics of immigration in OECD countries
Authors: Edo Anthony , Ragot Lionel , Rapoport Hillel , Sardoschau Sulin , Steinmayr Andreas , Sweetman Arthur
Published: November 2020

The share of the foreign‐born population in member countries of the OECD is increasing, and this article summarizes economics research on the effects of immigration in those nations. Four broad topics are addressed: labour market issues, fiscal questions, the political economy of immigration, and productivity and international trade. Extreme concerns about deleterious labour market and fiscal impacts following from new immigrants are not found to be warranted. However, it is also clear that government policies and practices regarding the selection and integration of new migrants affect labour market, fiscal, social and cultural outcomes. Policies that are well informed, well crafted and well executed beneficially improve population welfare.
Overtreatment as an ethical dilemma in Australian private dentistry: A qualitative exploration
Authors: Holden Alexander C. L. , Adam Lee , Thomson W. Murray
Published: November 2020

Background

The phenomenon of overtreatment in dentistry is under‐explored and has not been the subject of much research inquiry. Most dental care in Australia is provided by practitioners in private dental practice. This research expands understanding of how the nexus between professionalism and commercialism in dentistry is impacted by the phenomenon of overtreatment.

Methods

Data were collected through recorded interviews and written reflections from private dentists working in the Australian context. The data were then examined using thematic analysis to understand common themes and reveal deeper insights.

Results

A total of 20 participants were recruited for this study. Participants spoke of their experiences in practice where financial targets and pressure from practice owners to provide high‐cost treatments presented challenges to their professionalism. The participants expressed that the way in which dentistry is remunerated based on activity was a motivation towards engaging in overtreatment. The participants also spoke of finding a balance between commercialism and professionalism within private dental practice.

Conclusions

Participants’ reports provided insights into how the mismanagement of the commercial elements of private dental practice led to a transactional (‘buyer beware’) relationship between the consumers and providers of dental services, rather than a relationship founded upon professional altruism and trust. Consumer empowerment through the commercialization of dentistry has potential to be a positive influence on the patient experience in accessing dental care. However, this research has shown that the shift of power through consumerism also risks attenuating professional appreciation of the imbalanced nature of the patient‐clinician relationship in dentistry.
SARS‐CoV‐2 and COVID‐19 for the ophthalmologist
Authors: Brandão‐de‐Resende Camilo , Diniz‐Filho Alberto , de Almeida Brito Fabiano , Vasconcelos‐Santos Daniel Vitor
Published: November 2020

The COVID‐19 pandemic has had an unprecedented impact on ophthalmology. This review compiles general aspects of the novel coronavirus and COVID‐19, further dissects the most recent data on the role of the eye regarding disease transmission and manifestations, and summarizes preventive measures in the particular context of eye care.
Expanding the molecular spectrum and the neurological phenotype related to CAMTA1 variants
Authors: Jacobs Eva Z. , Brown Kathleen , Byler Melissa C. , D'haenens Erika , Dheedene Annelies , Henderson Lindsay B. , Humberson Jennifer B. , van Jaarsveld Richard H. , Kanani Farah , Lebel Robert Roger , Millan Francisca , Oegema Renske , Oostra Ann , Parker Michael J. , Rhodes Lindsay , Saenz Margarita , Seaver Laurie H. , Si Yue , Vanlander Arnaud , Vergult Sarah , Callewaert Bert
Published: November 2020

The CAMTA1‐associated phenotype was initially defined in patients with intragenic deletions and duplications who showed nonprogressive congenital ataxia, with or without intellectual disability. Here, we describe 10 individuals with CAMTA1 variants: nine previously unreported (likely) pathogenic variants comprising one missense, four frameshift and four nonsense variants, and one missense variant of unknown significance. Six patients were diagnosed following whole exome sequencing and four individuals with exome‐based targeted panel analysis. Most of them present with developmental delay, manifesting in speech and motor delay. Other frequent findings are hypotonia, cognitive impairment, cerebellar dysfunction, oculomotor abnormalities, and behavioral problems. Feeding problems occur more frequently than previously observed. In addition, we present a systematic review of 19 previously published individuals with causal variants, including copy number, truncating, and missense variants. We note a tendency of more severe cognitive impairment and recurrent dysmorphic features in individuals with a copy number variant. Pathogenic variants are predominantly observed in and near the N‐ and C‐ terminal functional domains. Clinical heterogeneity is observed, but 3′‐terminal variants seem to associate with less pronounced cerebellar dysfunction.
High ligation of the inferior mesenteric artery and anastomotic leakage in anterior resection for rectal cancer: a systematic review and meta‐analysis of randomized controlled trial studies
Authors: Kong Meng , Chen Hongyuan , Xin Yingying , Jiang Yugang , Han Yue , Sheng Hongguang
Published: November 2020

Aim

Surgeons have concerns whether high ligation (HL) of the inferior mesenteric artery (IMA) increases the incidence of anastomotic leakage (AL). This meta‐analysis aimed to evaluate the influence of HL of the IMA on AL compared with low ligation (LL).

Methods

PubMed, Embase, Web of Science, Cochrane Central Register of Controlled Trials and ClinicalTrials.gov databases were searched. Randomized controlled trial studies that compared HL with LL of the IMA in anterior resection for rectal cancer and reported AL outcomes were eligible for inclusion. The odds ratios and mean differences were analysed by a random‐effects model. Trial sequential analysis was performed to minimize the risk of random errors. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach was used to evaluate the quality of evidence for outcomes.

Results

Of the 531 records screened, five randomized controlled trials with 779 patients were selected for analysis. The pooled incidence of AL was 12.1% (95% Cl 7.77–18.26) in the HL group and 9.7% (95% Cl 5.79–15.82) in the LL group (OR 1.20, 95% CI 0.77–1.87, P = 0.42). In trial sequential analysis, the cumulative Z‐score curve exceeded the futility boundary, although the required information size of 1060 had not been reached. The quality of evidence was judged to be high according to the GRADE approach.

Conclusions

This meta‐analysis shows that HL of the IMA does not increase the incidence of AL in anterior resection for rectal cancer.