bluuebunny/crossref_metadata_2025 huggingface dataset:
Potensi Energi Batubara serta Pemanfaatan dan Teknologinya di Indonesia Tahun 2020 – 2050 : Gasifikasi Batubara
Authors: Afin Anugrah Pratama , Kiono Berkah Fajar Tamtomo
Published: November 2021

Sebagai salah satu sumber daya alam yang melimpah di Indonesia, Batubara berdasarkan data yang diperoleh dari Badan Geologi Kementerian ESDM, potensi dan cadangan batubara sebesar 186 miliar ton yang ada di Indonesia dimana 52 persen berada di Pulau Sumatera , 47 persen berada di Pulau Kalimantan dan 1 persen berada di Pulau lainnya. Pada saat ini pemanfaatan sumber energi batubara juga semakin meningkat seiring dengan menurunya produksi minyak bumi. Maka saat ini banyak Industri yang mulai mengalihkan focus energi nya ke Batubara. Dewasa ini pemakaian batubara yang terbanyak adalah pada sektor pembangkit listrik, pabrik semen dan industri lainnya dimana hampir separuh konsumsi batubara domestik dipergunakan sebagai bahan bakar pembangkit listrik. Pemanfaatan batubara juga akan semakin dominan dengan adanya kebijakan energi nasional sementara itu produksi energi fosil yang lain seperti minyak bumi dan gas bumi mengalami penurunan akibat cadangan yang semakin menipis ditambah pula dari data bauran energi nasional masih didominasi oleh bahan bakar minyak maka bukan tidak mungkin harganya akan terus semakin meningkat oleh karena itu dengan potensi batubara yang ada untuk mulai dilakukan subtitusi dan diversifikasi terhadap bahan bakar minyak dan gas bumi menjadi menarik dikarenakan masih besarnya potensi batubara di Indonesia. Adapun teknologi yang digunakan yaitu diantaranya gasifikasi dimana teknologi ini mulai gencar dimulai maka dalam tulisan ini dicoba meninjau perkembangan gasifikasi serta pemanfaatan teknologi , kapasitas dan kondisi saat ini yang ada di Indonesia.
The anti-fatigue potential of water-soluble polysaccharides of Semen cassiae on BALB/c mice
Authors: Kang Chenzhe , Liu Yanan , Chi Aiping , Zhang Zilin
Published: November 2021

Fatigue syndrome is a major health problem that affects the voluntary activities of an individual. Particularly, exercise-induced fatigue has become a serious concern in people's health. Since polysaccharides from various medicinal plants have been reported for anti-fatigue effect, the current study deals with the anti-fatigue potential of water-soluble polysaccharides of the Chinese medicinal plant Semen cassiae (Cassia obtusifolia L.) in BALB/c mice. Water-soluble polysaccharides from Semen cassiae were extracted using aqueous solvent (water). An orthogonal test design was employed for the optimization of polysaccharide extraction. The conditions optimized through this design unveiled the raw materials to solvent ratio as 1:30. The optimal temperature and time duration were found to be 80°C and 3.5 h, respectively. The yield of soluble polysaccharides at these specified conditions was 5.42%. Strikingly, the water-soluble polysaccharide from S. cassiae exhibited strong anti-fatigue activity at 100 mg/kg in BALB/c mice. S. cassiae polysaccharide extended the weight-loaded swimming duration in BALB/c mice. In addition, it ameliorated the level of antioxidant enzymes (SOD, GPX) while decreased the blood urea nitrogen, creatine phosphokinase, triglyceride, lactic acid, lactate dehydrogenase, and malondialdehyde levels in blood serum. Moreover, the assessment of the immunomodulatory effect of S. cassia polysaccharides unveiled the enhancement of B-cell and T-cell lymphocytes, denoting the positive effect on physical immunity.
snoRNA23 enhances the progression of hepatocellular carcinoma via regulation of the Wnt/β-catenin pathway
Authors: Qiao Shengxian , Zhang Xu , Wu Zhichao , Zhang Lei , Sun Shaojie
Published: November 2021

Small nucleolar non-coding RNA(snoRA)23 is upregulated in human pancreatic ductal adenocarcinoma. However, to the best of our knowledge, the role of snoRA23 in hepatocellular carcinoma progression has not been determined. MTT and colony formation assays were used to assess the cell viability and proliferation of HCC cells with snoRA23 knocked down, respectively, and a lymphatic vessel formation assay was used to determine tube formation ability of Human dermal lymphatic endothelial cells treated with conditioned media from HCC cell cultures. The results showed that snoRA23 knockdown attenuated cell viability, colony formation,and lymphatic vessel formation in HCC cells. snoRA23 was correlated with the prolonged overall survival of patients with HCC. Additionally, snoRA23 knockdown downregulated the Wnt/β-catenin signaling pathway by decreasing Wnt3a expression and β-catenin levels.β-methylacyl-CoA racemase (AMACR) levels were notably decreased by snoRA23 depletion. Finally, it was confirmed that AMACR overexpression partially rescued snoRA23-modulated HCC tumorigenesis. The results of the present study provide further insight into the role of non-coding RNAs in the development and progression of HCC.
Expression of FOXA1 gene regulates the proliferation and invasion of human gastric cancer cells
Authors: Dai Yun , Yang Guangming , Yang Lie , Jiang Li , Zheng Guohua , Pan Shuyin , Zhu Chuming
Published: November 2021

Forkhead box (FOX) transcription factors regulate the development of several human cancers. However, the role and therapeutic potential of FOXA1 in gastric cancer is still largely unexplored. The results showed a significant (P < 0.05) upregulation of FOXA1 in gastric cancer tissues and cell lines. Silencing of FOXA1 in gastric cells significantly (P < 0.05) decreased their viability through induction of apoptosis. The induction of apoptosis was associated with upregulation of Bax and downregulation of Bcl-2. Additionally, FOXA1 silencing caused activation of caspase-3 and 9 with no apparent effects on the expression of caspase-8 suggestive of intrinsic apoptosis. The transwell cell invasion revealed significant (P < 0.05) decline of cell invasion of gastric cancer cells upon FOXA1 silencing. The FOXA1 knockdown further inhibited the in vivo tumor growth suggestive of its therapeutic potential. Taken together, the findings of the present revealed that FOXA1 regulates the proliferation and development of gastric cancer and may exhibit therapeutic implications in gastric cancer treatment.
The effect of antioxidants in Ehrlich Ascites Cancer
Authors: Yılmaz Seher , Tokpınar Adem , Eroğlu Ece , Ateş Şükrü , Zahid Rabbia , Avnioğlu Seda , Bozkurt Özlem , Nisari Mehtap
Published: November 2021

A fundamental goal in molecular oncology is to unravel the underlying mechanisms which cause the cell transformation. In line with this approach, genome-wide functional screening approaches have revealed exciting insights into heterogeneous nature of cancer. Rapidly expanding horizons of research have unraveled myriad of pathways which play instrumental role in carcinogenesis and metastasis. Oxidative stress has also been reported to be significantly involved in cancer onset and progression. In line with this approach, oxidative stress modulating chemicals have always been sharply divided into antioxidants and oxidative stress-inducing agents. Conceptual and experimental advancements have enabled us to critically analyze full potential of these two different groups of chemicals in cancer chemoprevention. Different antioxidants are currently being analyzed in different phases of clinical trials. Although it has been reported in the literature that antioxidant supplements reduce tumor cells in some tumors or cause volume reduction in solid tumor sizes, there is no definite consensus. Therefore, an antioxidant supplement guideline based on more detailed clinical research and as a result of these is needed to achieve the best care for cancer patients and to avoid risky treatments for cancer patients.
Regulation of cell signaling pathways by Schisandrin in different cancers: Opting for "Swiss Army Knife" instead of "Blunderbuss"
Authors: Lin Xiukun , Attar Rukset , Mobeen Iqra , Yulaevna Ishmuratova Margarita , Aras Aliye , Butt Ghazala , Farooqi Ammad Ahmad
Published: November 2021

There has been an exponential growth in the field of molecular oncology and cutting-edge research has enabled us to develop a better understanding of therapeutically challenging nature of cancer. Based on the mechanistic insights garnered from decades of research, puzzling mysteries of multifaceted nature of cancer have been solved to a greater extent. Our rapidly evolving knowledge about deregulated oncogenic cell signaling pathways has allowed us to dissect different oncogenic transduction cascades which play critical role in cancer onset, progression and metastasis. Pharmacological targeting of deregulated pathways has attracted greater than ever attention in the recent years. Henceforth, discovery and identification of high-quality biologically active chemicals and products is gaining considerable momentum. There has been an explosion in the dimension of natural product research because of tremendous potential of chemopreventive and pharmaceutical significance of natural products. Schisandrin is mainly obtained from Schisandra chinensis. Schisandrin has been shown to be effective against different cancers because of its ability to inhibit/prevent cancer via modulation of different cell signaling pathways. Importantly, regulation of non-coding RNAs by schisandrin is an exciting area of research that still needs detailed and comprehensive research.   However, we still have unresolved questions about pharmacological properties of schisandrin mainly in context of its regulatory role in TGF/SMAD, SHH/GLI, NOTCH and Hippo pathways.
Astragalin flavonoid inhibits proliferation in human lung carcinoma cells mediated via induction of caspase-dependent intrinsic pathway, ROS production, cell migration and invasion inhibition and targeting JAK/STAT signalling pathway
Authors: Xu Guoxing , Yu Biqing , Wang Rujuan , Jiang Jie , Wen Fangjing , Shi Xiaowu
Published: November 2021

The aim of the current study was to investigate the anti-lung cancer effects of astragalin. Studies were also undertaken to evaluate its effects on apoptosis induction, ROS production, cellular migration and invasion and JAK/STAT3 signalling pathway. MTT assay was used to evaluate cell viability in NSCLC A549 cells after exposure to astragalin molecule. Apoptosis was investigated using AO/EB staining, comet assay and western blotting assay. Fluorescence microscopy was implemented to estimate ROS production. Cell migration and invasion were measured using transwell chambers assay. Effects of astragalin on JAK/STAT pathway were investigated using western blotting assay. Results showed astragalin molecule induced inhibition of proliferation in A549 cells in a dose-dependent fashion. Further, the antiproliferative effects were found to mediate via apoptosis as suggested by AO/EB staining and western blotting assay. Astragalin modulated the expressions of caspase-3, caspase-9, Bax, Bak, Cyt-c Bcl-2, XIAP and Bcl-xL. Astragalin induced DNA damage in A549 cells which too indicated apoptotic cell death. Astragalin molecule enhanced the production of ROS by A549 cells. It inhibited both cell migration and invasion of A549 cells in a concentration-dependent manner. Finally, astragalin drug was observed with remarkable potential of targeting JAK/STAT pathway in A549 NSCLC cells. These results indicated that astragalin drug could prove helpful in lung cancer treatment and research provided more in-vivo studies are performed.
Investigating platelet-activating factor as a potent proinflammatory mediator in coronary atherosclerotic patients
Authors: Raheem Shler Ghafoor
Published: November 2021

The inflammatory reaction is one of the complications in patients with coronary atherosclerosis. This study aimed to determine the diagnostic value of platelet-activating factor (PAF) compared with high sensitivity C reactive protein (hs-CRP) in coronary atherosclerotic patients. Fifty patients with coronary atherosclerosis and 30 subjects with normal angiography were considered as the control group attending Cardiac Center-Surgical Specialty Hospital - in Erbil city / Iraq. The levels of PAF and hs-CRP were estimated quantitatively using a sandwich enzyme-linked immunosorbent assay and a particle-enhanced immune turbid metric assay, respectively. Lipid profiles and some hematological indexes were also used in this study. The levels of the inflammatory biomarkers of PAF and hs-CRP increased significantly in the patients group compared with controls (p<0.05). Although the patients group showed the highest level of low-density lipoprotein (LDL), the difference was not significant (p>0.05) compared with the healthy control. However, the incidence of risk factors such as smoking and obesity showed a significant difference (p<0.05) in the patients group. Additionally, the PAF level correlated positively and significantly with hs-CRP (p<0.05), and negatively with high-density lipoprotein (HDL) (p>0.05). Although hs-CRP was a valuable diagnostic marker for coronary atherosclerosis, the PAF level showed to be a better prognostic indicator than hs-CRP in coronary atherosclerosis patients.
Prognostic relevance of combined IDH1 and NPM1 mutations in the intermediate cytogenetic de novo acute myeloid leukemia
Authors: Abd El-Lateef Amal Ezzat , Ismail Manar M. , Almohammadi Mohammed , Gawaly Amr M.
Published: November 2021

Despite the great advance in treatment, cytogenetically normal Acute myeloid leukemia (CN-AML) is still a challenging entity. The discovery of IDH1 mutation in AML together with the frequent co-mutations; NPM1 and FLT3-ITD throughs a new insight into the pathogenesis and outcome of CN-AML. Recently, there has been an increasing number of recurring mutations in other genes for which the forecasting effect is still required. Despite the large number of risk variables established, there are relatively few prognostic indicators that can help in treatment decisions in AML patients. This study aimed at recording the frequency of IDH1 and NPM1 mutations in newly diagnosed AML and, dual clinicopathological significance. IDH1 and NPM1 mutations were analyzed using High-Resolution Melting curve analysis PCR in 78 newly diagnosed AML patients; 30 pediatric and 48 adult AML patients. IDH1 mutation was detected in 6 out of the 48 adult AML cases (12.5%) and all of them had intermediate cytogenetic prognostic stratification. 5/6 mutant IDH1 patients showed NPM1 co-mutation (P-value= 0.008). Mutant IDH1 patients showed significant resistance to induction therapy (P-value <0.001) and even those who achieved complete remission were relapsed later. Within the intermediate cytogenetic group, the IDH1 mutated patients had short overall survival (HR 12.9, 95% CI (3.1- 53.45) and event-free survival (HR 15.7, 95% CI (2.99-82.72) and P-value <0.001). IDH1 mutation is closely linked to the intermediate cytogenetic stratified group and in particular old age patients and has a great impact on their survival.
A poly (saccharide-ester-urethane) scaffold for mammalian cell growth
Authors: González-Torres Maykel , Becerra-González Marymar , Leyva-Gómez Gerardo , Lima Enrique , González Mendoza Oswaldo , Ruvalcaba-Paredes Erika Karina , Cortés Hernán , Pineda Carlos , Martínez-Torres Ataúlfo
Published: November 2021

Chitosan and poly(3-hydroxybutyrate) are non-toxic, biodegradable, and biocompatible polymers extensively used in regenerative medicine. However, it is unknown whether the chemical combination of these polymers can produce a biomaterial that induces an appropriate cellular response in vitro in mammalian cells. This study aimed to test the ability of a novel salt-leached polyurethane scaffold of chitosan grafted with poly(3-hydroxybutyrate) to support the growth of three mammalian cell lines of different origin: a) HEK-293 cells, b) i28 mouse myoblasts, and c) human dermal fibroblasts. The viability of the cells was assessed by either evaluation of their capacity to maintain the expression of the green fluorescent protein by adenoviral transduction or by esterase activity and plasma membrane integrity. The results indicated that the three cell lines attached well to the scaffold; however, when i28 cells were induced to differentiate, they did not produce morphologically distinct myofibers, and cell growth ceased. In conclusion, the findings reveal that, altogether, these observations suggest that this foam scaffold supports cell growth and proliferation but may not apply to all cell types. Hence, one crucial question yet to be resolved is a poly (saccharide-ester-urethane) derivative with a nano-topography that elicits a similar cellular response for different biological environments.
Serum levels of immunoglobulin and complement in UTI of patients caused by Proteus mirabilis and using AgNPs as antiswarming
Authors: Al Otraqchi Kamal Ismael Bakr , Darogha Suhaila Nafee , Ali Beriwan Abdulqadir
Published: November 2021

The use of plant extracts represents a promising approach for the synthesis of silver nanoparticles (AgNPs). This study reports the low-cost, green synthesis of AgNPs using the extract of clove and black seeds. The biosynthesized AgNPs were confirmed and characterized by analysis of the spectroscopy profile of the UV-visible spectrophotometer. The purpose of the present study is to evaluate the inhibitory effect concentration (MIC) of AgNPs, clove, and black cumin seed extracts on the growth and swarming of P. mirabilis. Clinical isolates of P. mirabilis were isolated from patients suffering from urinary tract infections. Thirteen types of antibiotics were used in the present study to detect their ability to inhibit P. mirabilis's resistance. Immunological findings included the determination of serum levels of IgG, IgM, IgA and complement protein C3 and C4. Results showed that IgG and IgA concentrations significantly increased (1311.13 ± 72.54 and 279 ± 21.31) respectively in UTI patients in comparison to the healthy control group which was 1089.88 ± 37.33 and 117.611 ± 4.19 respectively, While IgM concentrations were increased non significantly in UTI patients (153.331 ± 6.45) in comparison to healthy control (145.2 ± 13.49). Complement components C3 showed a significant increase in UTI patients with mean values of 125.95 ± 6.22 compared to the control group with mean values of 55.191 ± 9.64, while C4 showed statically non-significant among UTI patients in comparison with the control group (35.195 ± 2.34 and 34.371 ± 1.22) respectively.
Overview of the signaling pathways involved in metastasis: An intriguing story-tale of the metastatic journey of ovarian cancer cells
Authors: Attar Rukset , Panah Tahira , Romero Mirna Azalea , Yulaevna Ishmuratova Margarita , Gazouli Maria , Berardi Rossana , Wieczorek Edyta , Farooqi Ammad Ahmad
Published: November 2021

Wealth of information has revolutionized our understanding related to the genetics and functional genomics of this heterogeneous disease. Keeping in view the heterogeneity of ovarian cancer, long-term survival might be achieved by translation of recently emerging mechanistic insights at the cellular and molecular levels to personalize individual strategies for treatment and to identify biomarkers for early detection. Importantly, the motility and invasive properties of ovarian cancer cells are driven by a repertoire of signaling cascades, many components of which have been experimentally verified as therapeutic targets in preclinical models as well as in clinical trials. Scientific evidence garnered over decades of research has deconvoluted the highly intricate intertwined network of intracellular signaling pathways which played fundamental role in carcinogenesis and metastasis. In this review we have provided a compendium of myriad of signaling cascades which have been documented to play critical role in the progression and metastasis of ovarian cancer. We have partitioned this multi-component review into different sections to individually discuss and summarize the roles of TGF/SMAD, JAK/STAT, Wnt/?-Catenin, NOTCH, SHH/GLI, mTORC1/mTORC2, VEGFR and Hippo/YAP pathways in ovarian cancer metastasis.
ZnO, TiO2 and Ag nanoparticles impact against some species of pathogenic bacteria and yeast
Authors: Kareem Payman Ali , Salh Khonaw Kader , Ali Fattma A.
Published: November 2021

The economic approaches for manufacturing the nanoparticles with physical and chemical effects and limited resistance to antibiotics have been progressed recently due to the rise of microbial resistance to antibiotics. This research aimed to study the antimicrobial efficacy of silver nanoparticles Ag, ZnO, and Tio2 nanoparticles against Salmonella typhimurium and Brucella abortus and Candida albicans. Two isolates of Salmonella and two isolates of Brucella abortus were isolated from food spastically meat and blood specimens, respectively. Candida albicans were isolated from the patient's mouth with oral candidiasis (oral thrush) and confirmed diagnosis by API 20C test. The antimicrobial susceptibility of Salmonella typhimurium and B. abortus isolates were performed against nine different antibiotics. Silver nanoparticles consisting of AgNPs size (90) nm, ZnO NPs size (20, 50) nm as well as TiO2 NPs size (10, 50) nm, were used. UV-Visible spectrophotometer was used to characterize silver nanoparticles. The highest resistance of Candida albicans was seen for fluconazole, Clotrimazole and Itraconazole. The results of the Minimum Inhibitory Concentration (MIC) of nanoparticles against Salmonella typhimurium showed the average MIC of Tio2-10nm and Tio2-50nm were 5000 and 2500 ?g\ml for S1 and S2 isolates, respectively. The isolated Brucella abortus (B1 and B2) showed sensitivity to NPs with different MIC. The average MIC for Ag-90nm was 5000 and 2500 µg/ml for B1 and B2 isolates, respectively. The findings suggest Ag solution has fungicidal and bactericidal impacts on the tested microorganisms so they can be suitable for multiple applications of the biomedical field such as developing new antimicrobial agents.
Exonic sequencing and MLH3 gene expression analysis of breast cancer patients
Authors: Khailany Rozhgar A. , Ozaslan Mehmet
Published: November 2021

Breast cancer is the most common cancer in women worldwide. Detection of breast cancer susceptibility genes is an important issue. Also, MLH3 is a DNA mismatch repair gene and mutation in this gene is harmful in different cancers. This study aimed to use exome sequencing to uncover previously undetected breast cancer-predisposing variants. Also, we investigated the MLH3 gene expression of breast cancer patients which can be a breast cancer susceptibility gene. A total of 80 samples including 40 paired normal and cancer tissue samples were collected at Zheen International Hospital, Erbil, Iraq. Exome sequencing was used to identify mutations. Different in silico tools were used to predict the effect of mutation on the structural features or protein function. Real-time PCR was used for assessing the expression of MLH3 in breast cancer patients. We identified 26 variants in breast cancer patients, 22 inherited variants were found in MLH3, CHECK2, BRCA1, BRCA2, BLM, TP53, MSH6, NBN and PTEN genes and 4 somatic variants were found in PALB2, RAD50 and RBM10 genes. It was found that the expression of the MLH3 gene in tumor samples was significantly down-regulated compared with normal tissues. Statistically, high significance was found. The decreased expression of MLH3 was significant in all ranges of ages and all breast cancer types. Also, the expression of MLH3 decreased significantly in patients with breast cancer grades of II and III. In conclusion, MLH3 can be used as a susceptibility gene especially in grades II and III of breast cancer.
Catalonia 2018: Clashing Identities in Public Space
Authors: Kubiaczyk Filip
Published: November 2021

The article analyses the manifestations of underlying identity conflicts in the social spaces of Catalonia, chiefly Barcelona, encountered in the streets, on the sidewalks, motorways, buildings, or beaches. The study focuses on elements that made their appearance after the illegal Catalan independence referendum of October 1st, 2017. Relying on participant observation, the author shows Catalan public space as a domain of semantic and polysemous game, in which struggle for freedom and free speech is the core element.
Racjonalność działania i jej ograniczenia
Authors: Moraczewski Krzysztof
Published: November 2021

The article is discusses possible limitations of the rationalistic concept of human action within cultural theory. Here, rationality of human action is construed in terms of decision theory. Two kinds of limitations are considered: (1) resulting from non-rationalistic conceptions of human action; (2) implied by anti individualistic approaches to socio-cultural phenomena, especially structuralist and functionalist ones. Proposed conclusions aim for a reconciliation of rationalistic theory of action and principal premises of cultural materialism.
Generalized split null point of sum of monotone operators in Hilbert spaces
Authors: Mebawondu Akindele A. , Abass Hammed A. , Oyewole Olalwale K. , Aremu Kazeem O. , Narain Ojen K.
Published: November 2021

In this paper, we introduce a new type of a generalized split monotone variational inclusion (GSMVI) problem in the framework of real Hilbert spaces. By incorporating an inertial extrapolation method and an Halpern iterative technique, we establish a strong convergence result for approximating a solution of GSMVI and fixed point problems of certain nonlinear mappings in the framework of real Hilbert spaces. Many existing results are derived as corollaries to our main result. Furthermore, we present a numerical example to support our main result and propose an open problem for interested researchers in this area. The result obtained in this paper improves and generalizes many existing results in the literature.
Legislative discourse of digital governance: a corpus-driven comparative study of laws in the European Union and China
Authors: Li Siyue , Kit Chunyu
Published: November 2021

Based on the self-compiled corpora of the European Union and Chinese laws on data governance, this study adopts a corpus-driven approach to comparatively study the legislative design of the EU and China on digital governance, especially on key issues such as data protection, data processing and utilization, and cross-border data transfer. It is found through corpus analysis that the EU has developed a relatively comprehensive data protection system, which internally focuses on the protection of individual data rights and externally sets high standards on the cross-border transfer of data. Despite the data protection paradigm as it manifests, the EU is facing new challenges on data exportation, data jurisdiction in the competitive digital marketplace. Shared the same concern on the data protection legislation, Chinese data law has made significant progress in personal data protection with the nascent enactment of Data Security Law and Personal Data Protection Law. Notably, Chinese legislation features the hierarchal taxonomy of data under the principle of the national security exception, while it requires more legislative skills, flexible response mechanisms, and more subordinate laws to prevent future data security threats. Moreover, the corpus-driven method conducted in this study provides evidential insights for the comparative legal textual studies across jurisdictions.
AOA ophthalmology and otolaryngology program closures as a model to highlight challenges of maintaining GME in high need areas
Authors: Ahmed Harris , Vo Kim , Robbins Wayne
Published: November 2021

Context

While 90% of former American Osteopathic Association (AOA) residency programs transitioned to Accreditation Council for Graduate Medical Education (ACGME) accreditation, surgical subspecialty programs such as ear, nose, and throat (ENT, 62%) and ophthalmology (47%) struggled to gain accreditation. Doctors of Osteopathic Medicine (DOs) actively participate in serving underserved communities, and the loss of AOA surgical specialty programs may decrease access to surgical care in rural and nonmetropolitan areas.

Objectives

To determine the challenges faced by former AOA-accredited surgical subspecialty programs during the transition to ACGME accreditation, particularly ENT and ophthalmology programs in underresourced settings.

Methods

A directory of former AOA ENT and Ophthalmology programs was obtained from the American Osteopathic Colleges of Ophthalmology and Otolaryngology-Head and Neck Surgery (AOCOO-HNS). A secured survey was sent out to 16 eligible ENT and ophthalmology program directors (PDs). The survey contained both quantitative and qualitative aspects to help assess why these programs did not pursue or failed to receive ACGME accreditation.

Results

Twelve of 16 eligible programs responded, com-prising six ophthalmology and six ENT PDs. Among the respondents, 83% did not pursue accreditation (6 ophthalmology and 4 ENT programs), and 17% were unsuccessful in achieving accreditation despite pursuing accreditation (2 ENT programs). Across 12 respondents, 7 (58%) cited a lack of hospital/administrative support and 5 (42%) cited excessive costs and lack of faculty support as reasons for not pursuing or obtaining ACGME accreditation.

Conclusions

The survey results reflect financial issues associated with rural hospitals. A lack of hospital/administrative support and excessive costs to transition to the ACGME were key drivers in closures of AOA surgical specialty programs. In light of these results, we have four recommendations for various stakeholders, including PDs, Designated Institutional Officials, hospital Chief Medical Officers, and health policy experts. These recommendations include expanding Teaching Health Center Graduate Medical Education to surgical subspecialties, identifying and learning from surgical fields such as urology that fared well during the transition to ACGME, addressing the lack of institutional commitment and the prohibitive costs of maintaining ACGME-accredited subspecialty programs in underresourced settings, and reconsidering the Centers for Medicare & Medicaid Services (CMS) pool approach to physician reimbursement.
Tetradentate organophosphines in Pt(η4–A4L) (A = P4, P3Si, P2X2 (X2 = N2, S2, C2), PX3 (X3 = N3, N2O)): Structural aspects
Authors: Melník Milan , Mikuš Peter
Published: November 2021

We report herein structural characterization of monomeric platinum complexes of the composition: Pt(η4–P4L), Pt(η4–P3SiL), Pt(η4–P2N2L), Pt(η4–P2S2L), Pt(η4–P2C2L), Pt(η4–PN3L), and Pt(η4–PN2OL). The tetradentate ligands with 10-, 11-, 12-, 14-, and 16-membered macrocycles create a variety of chelate bond angles. A distorted square-planar geometry about Pt(II) atoms with cis–configuration by far prevail. There is an example Pt(η4–P3SiL) in which the respective donor atoms build up a trigonal-pyramidal geometry about Pt(II) atom.
Chronotopic translanguaging and the mobile languaging subject: insights from an Algerian academic sojourner in the UK
Authors: Taibi Hadjer , Badwan Khawla
Published: November 2021

This study discusses the impact of spatial, temporal and virtual mobility on how mobile individuals talk about language in their world, and how they use language offline and online to communicate over time and across space. We introduce the notion ofchronotopic translanguagingto highlight the significance of merging time and place in sociolinguistics. Doing so, we present a rather stretched understanding of time to include references to real time, online compressed time, linguistic ideologies and practices carried over time and challenged in recent times, as well as understanding time as an ecological factor. We interviewed Ekram, an Algerian academic sojourner, and observed her Facebook profile before and after coming to the UK. Our findings suggest that the networked lives of the participant beget fluid translanguaging practices that are constantly (re)negotiated depending to the ecology of interaction. Through entering and existing multiple time-space frames, Ekram found herself reunited with communicative repertoires she has not used for years. She also developed new relationships with other repertoires. This study concludes by emphasising the usefulness ofchronotopic translanguagingas a conceptual tool that permits, and accounts for, the time-place influence on how mobile individuals deploy their communicative repertoires.
Angiotensin(1–7) activates MAS-1 and upregulates CFTR to promote insulin secretion in pancreatic β-cells: the association with type 2 diabetes
Authors: Zhang Xue-Lian , Zhao Xinyi , Wu Yong , Huang Wen-qing , Chen Jun-jiang , Hu Peijie , Liu Wei , Chen Yi-Wen , Hao Jin , Xie Rong-Rong , Chan Hsiao Chang , Ruan Ye Chun , Chen Hui , Guo Jinghui
Published: November 2021

Objective

The beneficial effect of angiotensin(1–7) (Ang(1–7)), via the activation of its receptor, MAS-1, has been noted in diabetes treatment; however, how Ang(1–7) or MAS-1 affects insulin secretion remains elusive and whether the endogenous level of Ang(1–7) or MAS-1 is altered in diabetic individuals remains unexplored. We recently identified an important role of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated Cl− channel, in the regulation of insulin secretion. Here, we tested the possible involvement of CFTR in mediating Ang(1–7)’s effect on insulin secretion and measured the level of Ang(1–7), MAS-1 as well as CFTR in the blood of individuals with or without type 2 diabetes.

Methods

Ang(1–7)/MAS-1/CFTR pathway was determined by specific inhibitors, gene manipulation, Western blotting as well as insulin ELISA in a pancreatic β-cell line, RINm5F. Human blood samples were collected from 333 individuals with (n  = 197) and without (n  = 136) type 2 diabetes. Ang(1–7), MAS-1 and CFTR levels in the human blood were determined by ELISA.

Results

In RINm5F cells, Ang(1–7) induced intracellular cAMP increase, cAMP-response element binding protein (CREB) activation, enhanced CFTR expression and potentiated glucose-stimulated insulin secretion, which were abolished by a selective CFTR inhibitor, RNAi-knockdown of CFTR, or inhibition of MAS-1. In human subjects, the blood levels of MAS-1 and CFTR, but not Ang(1–7), were significantly higher in individuals with type 2 diabetes as compared to those in non-diabetic healthy subjects. In addition, blood levels of MAS-1 and CFTR were in significant positive correlation in type-2 diabetic but not non-diabetic subjects.

Conclusion

These results suggested that MAS-1 and CFTR as key players in mediating Ang(1–7)-promoted insulin secretion in pancreatic β-cells; MAS-1 and CFTR are positively correlated and both upregulated in type 2 diabetes.
20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor
Authors: Lafont René , Serova Maria , Didry-Barca Blaise , Raynal Sophie , Guibout Louis , Dinan Laurence , Veillet Stanislas , Latil Mathilde , Dioh Waly , Dilda Pierre J
Published: November 2021

20-Hydroxyecdysone (20E) is a steroid hormone that plays a key role in insect development through nuclear ecdysteroid receptors (EcR/RXR complex) and at least one membrane GPCR receptor (DopEcR). It also displays numerous pharmacological effects in mammals, where its mechanism of action is still debated, involving either an unidentified GPCR or the estrogen ERβ receptor. The goal of this study was to better understand 20E mechanism of action in mammals. A mouse myoblast cell line (C2C12) and the gene expression of myostatin (a negative regulator of muscle growth) were used as a reporter system of anabolic activity. Experiments using protein-bound 20E established the involvement of a membrane receptor. 20E-like effects were also observed with angiotensin(1–7), the endogenous ligand of MAS. Additionally, the effect on myostatin gene expression was abolished by Mas receptor knock-down using siRNA or pharmacological inhibitors. 17β-Estradiol (E2) also inhibited myostatin gene expression, but protein-bound E2 was inactive, and E2 activity was not abolished by angiotensin(1–7) antagonists. A mechanism involving cooperation between the MAS receptor and a membrane-bound palmitoylated estrogen receptor is proposed. The possibility to activate the MAS receptor with a safe steroid molecule is consistent with the pleiotropic pharmacological effects of ecdysteroids in mammals and, indeed, the proposed mechanism may explain the close similarity between the effects of angiotensin(1–7) and 20E. Our findings open up many possible therapeutic developments involving stimulation of the protective arm of the renin–angiotensin–aldosterone system (RAAS) with 20E.
Tertiary Amine-Mediated Polyvinyl Alcohol Membrane Over the Porous Support of Polyvinyl Chloride Membrane for CO2 Separation
Authors: Asim Mushtaq , Saud Hashmi , Saad Nadeem , Zahoor Awan , Zaeem Uddin Ali
Published: November 2021

Great attention has been paid to membrane-based separation technology in various separation fields, including gas separation. It provides the benefits of energy efficiency, environmental friendliness, easy scale-up, and convenience in operation. Different division advancements are being utilized for the expulsion of acid gas carbon dioxide (CO2). The aim of this work is to synthesis the membrane using polyvinyl alcohol (PVA) with treatment (WT) and without treatment (WOT) of the additive that is triethanolamine (TEA), to study the effect of additive on the permeance of membrane towards CO2 and the morphology changes of each membrane. In this research, virgin PVA and PVA with TEA were cast upon the porous support membrane of polyvinyl chloride (PVC). PVA was used as the polymer matrix, and TEA was used as a CO2 facilitating agent. Distilled water was used as a solvent for TEA and PVA in preparing the solution. Dimethyl acetamide (DMAc) and Tetrahydrofuran (THF) were used as solvents for PVC porous membranes. These membranes were tested on CO2 to find out the permeability and flux rates (J). For the morphology of the membrane, we performed SEM; for thermal analysis, we performed DSC and TGA, and for the strength, we performed the tensile test. The results reveal that the presence of TEA changes the morphology and thermal behavior increases the strength and the permeability of CO2. In a nutshell, the presence of TEA enhanced the performance and the morphology of the membrane.
THE STUDY OF NITRIC OXIDE ACTION IN VIVO ON NA+ , K+ -ÀÒPASE IN RAT AORTA
Authors:   O.O. Bogomoletz Institute of Physiology National Academy Sciences of Ukraine, Kyiv, Ukraine, Akopova O.V. , Kharlamova O.N. ,   O.O. Bogomoletz Institute of Physiology National Academy Sciences of Ukraine, Kyiv, Ukraine, Kotsiuruba A.V. ,   O.O. Bogomoletz Institute of Physiology National Academy Sciences of Ukraine, Kyiv, Ukraine, Korkach Yu.P. ,   O.O. Bogomoletz Institute of Physiology National Academy Sciences of Ukraine, Kyiv, Ukraine, Sagach V.F. ,   O.O. Bogomoletz Institute of Physiology National Academy Sciences of Ukraine, Kyiv, Ukraine
Published: November 2021

The influence of nitric oxide on Na+,K+-ATPase activity in rat aorta was studied by means of stimulation of endogenous NO synthesis after injections of bacterial lipopolysaccharide (LPS) and pharmacological NO donor nitroglycerine (NG). It was shown that NO action on Na+,K+-ATPase in vivo is dose-de­pendent. Stimulation of the endogenous NO synthesis by LPS as well as the administration of low doses of NG lead to the activation of Na+,K+-ATPase and favor the conclusion that NO-dependent Na+,K+-ATPase stimulation mediates vasodilatory and hypotensive action of nitric oxide. The Na+,K+-ATPase activity in rat aorta depends on the balance between the level of reactive oxygen and nitrogen species (ROS and RNS), forma­tion of NO depots in the tissue of aorta as high- and low mo­lecular weight nitrosothiols, and also on the intensity of free-radical reactions resulting in the generation of hydroperoxide radicals. The results obtained suggest that NOS- and cGMP-dependent pathway takes part in Na+,K+-ATPase activation by LPS and NG, but the enzyme inhibition by nitric oxide in vivo is not cGMP-dependent and is determined by the activation of free-radical reactions and dramatic enhancement of nitrosylation level in rat aorta tissue.
Mechanism of antiseizure effect of isoflurane in the immature rat hippocampus
Authors:   O.O.Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine, Isaeva. E.V.
Published: November 2021

The volatile anesthetic isoflurane is often used in children in the management of refractory status epilepticus. However the mechanism of anticonvulsant action of isoflurane during early brain development is not clear. In this study we explore the role of excitatory and inhibitory conductances in antiseizure effect of isoflurane using combination of whole-cell patch-clamp and extracellular field potential recording techniques on two models of epilepsy in a hippocampal slice preparation from immature rat. Our data demonstrated that decreasing of excitatory sy-naptic transmission does not account for antiseizure effect of this volatile anesthetic agent. Isoflurane decreases the synchro­nization of neuronal activity mainly through the enhancing of GABAergic inhibition by influencing both phasic and tonic chloride conductances.
Typification of representative philosophical and religious perceptions of Ukrainian internet audience
Authors:   Military Institute of Telecommunications and In-formation Technologies named after Heroes of Kruty 45/1, Moskovska Str., Kyiv, 01011, Ukraine, Khraban T. Ye.
Published: November 2021

The article aims to identify the particularities of philosophical and religious thinking inherent in the social network audience of Ukraine. Discourse analysis was chosen as the primary method to analyse a set of posts in the form of quotes with accompanying visual components and comments to them that were posted on “Facebook” in 2020 on the pages of public groups “Ukraine is Free World”, “For Ukraine”, “Dialogue.UA” and private groups “Ukrainians Global Network”, “Ukraine is You”, “Ukraine Onlineツ. The author analysed a total of 630 posts with God’s obligatory explicit or implicit component (The Higher Power), which bring out the existential issues of life, raison d’être, human values. The meaning of life is increasingly prominent in the philosophical and religious discourse of the Ukrainian sector of social networks. The idea of the meaning of life is presented on two levels: ideological and social. At the worldview level, ideas about the meaning of life are concentrated in the systemic principle: “Freedom is worth dying for”. At the social level, ideas about the meaning of life are concretised in the following concepts: socio-demographic, aesthetic, religious, hedonistic, hygge, success. The next most common issue is a subject of love understood by the Ukrainian audience of social networks as a system of traits: an active position with the other, value-based principles of a code of conduct, the meaning-making basis for self-realisation, recognising and acceptation the humanity of others, orientation on vital activity, goodwill, and unity, overcoming loneliness, a mode of self-determination. The subjects of time, different issues related to dying and death are ranked last. The tendency of philosophical and religious thinking has shaped the Ukrainian sector of social networks. It has the following special features: 1) view of the world and personal choice of life strategies is based on first-hand knowledge; 2) absence of abstract, unrealistic considerations; 3) strong link with a particular socio-cultural context; 4) focus on solving problems related to anthropological dimensions of philosophising: man as a unique being, the place of man in the world and his role in the processes of being, freedom and responsibility, time as a characteristic of human.
Axiological space of coevals of independence of Ukraine: sociological dimensions
Authors:   Ivan Franko National University of Lviv 1, Universitetska Str, Lviv, 79000, Ukraine, Chernysh N. Yo. , Boretska M. Yu. ,   Ivan Franko National University of Lviv 1, Universytetska Str., Lviv, 79000, Ukraine
Published: November 2021

The paper offers an attempt of theoretical conceptualisation of coevals’ values of independence of Ukraine, as well as carries out the approbation of the put forward theoretical positions in empirical studies with the participation of authors. Coevals of independence are the oldest age cohort of the generation born in free Ukraine, and the central line-forming concept is the concept of “generational values”. The authors emphasised that in the multiparadigmality of modern sociology, it is methodologically expedient to develop several variants of “generational values” concepts following the main lines of sociological theorising, each of which provides appropriate empirical research methods. Paper considers four possible research schemes of these concepts in the framework of the “first”, “second”, “third” sociology (based on the works of G. Zborovsky and P. Sztompka), and a scheme based on the work of K. Mannheim is singled out. Semantic modelling of the “generational values” concept of using the axiological core of the “first” sociology (or neoclassical, objectivist) metaparadigm of sociological theorising with the appropriate method of quantitative empirical research. This allowed testing the theoretical provisions of this concept in the development of methods and empirical research with the authors’ participation and a comparative analysis of sociological information with databases of the World and European Values Surveys 2020 (particularly in Ukraine). The common and distinctive features of axiological spaces of respondents aged 29–30 in the global, European, and local context are determined, the specifics of the axiological system of the older age cohort (coevals) of the generation born in 1991–1992 are revealed to emphasise the peculiarities of the value spaces of Ukraine’s independence coevals, first of all, in relation to the ratio of materialist and postmaterialist values. The general conclusion is the recorded ambivalence of the axiological space of thirty-year-old respondents with barely noticeable shifts towards the older generations in the context of the prevailing trend of transition to post-materialist values.
La transparencia y la rendición de cuentas en redes sociales. Un caso de conflictos mineros en Latinoamérica
Authors: Gómez-Villegas Mauricio , Suárez-Rico Yuli Marcela , Valenzuela-Jiménez Luis Fernando , García-Benau María Antonia
Published: November 2021

Diversos estudios muestran un creciente uso del Internet y las redes por parte de grandes empresas para divulgar información social y medioambiental, ampliando la rendición de cuentas y la transparencia. No obstante, aún no existe claridad sobre los pros y contras de dicho proceso. Este trabajo plantea la necesidad de una reinterpretación crítica de la transparencia y la rendición de cuentas sociales y medioambientales a través de las redes sociales. Para ello, retoma algunos argumentos de la perspectiva dialógica de la rendición de cuentas, así como planteamientos del filósofo coreano Byung-Chul Han. Se estudia el caso de la compañía minera Vale, caracterizando la forma en que las corporaciones utilizan las redes sociales argumentando mayor transparencia y rendición de cuentas sociales y medioambientales frente a graves catástrofes socioambientales. Sin embargo, el caso muestra que tal divulgación no visibiliza los conflictos intrínsecos de esta actividad, ni incorpora las visiones, expectativas y reclamos de los afectados.