bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimizing the Growth Conditions of the Selected Plant-Growth-Promoting Rhizobacteria Paenibacillus sp. MVY-024 for Industrial Scale Production
Authors: Kaziūnienė Justina , Mažylytė Raimonda , Krasauskas Aurimas , Toleikienė Monika , Gegeckas Audrius
Published: May 2022

In this study, thirteen isolates, which were possibly expected to fix nitrogen, were isolated from soil and pea root nodules and identified by the gene analysis of 16S rDNA sequences. Two of these isolates that were able to form endospores and grow on nitrogen-free media were selected for spring wheat development research. The isolate Paenibacillus sp. S7 identified as Paenibacillus polymyxa was found to significantly increase the amount of ammonium and mineral N amounts in the soil. Furthermore, increased nitrogen accumulation in grains and a chlorophyll index were obtained after wheat treatment. Paenibacillus sp. S7 isolate was selected for further studies and the accession number MT900581 and strain name MVY-024 in NCBI nucleotide bank for this isolate were assigned. During the cultivation of Paenibacillus sp. MVY-024, sugarcane molasses and a yeast extract were determined as the most suitable carbon and nitrogen sources, whose optimal concentrations were 100 g L−1 and 10 g L−1, respectively. The optimal pH range for the cell culture was between 6.5 and 7.0, and the optimal air flow rate was 0.4 vvm. It was found that the air flow has an effect on biomass production and endospore formation. After Paenibacillus sp. MVY-024 biomass cultivation optimization, the cultured cell number was, on average, 2.2 × 109 cfu m L−1.
Natural Products from Plants and Algae for Treatment of Alzheimer’s Disease: A Review
Authors: Klose Jana , Griehl Carola , Roßner Steffen , Schilling Stephan
Published: May 2022

Neurodegenerative disorders including Parkinson’s disease (PD), Huntington’s disease (HD) and the most frequent, Alzheimer’s disease (AD), represent one of the most urgent medical needs worldwide. Despite a significantly developed understanding of disease development and pathology, treatments that stop AD progression are not yet available. The recent approval of sodium oligomannate (GV-971) for AD treatment in China emphasized the potential value of natural products for the treatment of neurodegenerative disorders. Many current clinical studies include the administration of a natural compound as a single and combination treatment. The most prominent mechanisms of action are anti-inflammatory and anti-oxidative activities, thus preserving cellular survival. Here, we review current natural products that are either approved or are in testing for a treatment of neurodegeneration in AD. In addition to the most important compounds of plant origin, we also put special emphasis on compounds from algae, given their neuroprotective activity and their underlying mechanisms of neuroprotection.
High Expression of a tRNAPro Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence
Authors: Tsiakanikas Panagiotis , Adamopoulos Panagiotis G. , Tsirba Dimitra , Artemaki Pinelopi I. , Papadopoulos Iordanis N. , Kontos Christos K. , Scorilas Andreas
Published: May 2022

Colorectal cancer (CRC) is the second most lethal cause of cancer-related deaths in Europe. Fragments of tRNAPro are conserved among vertebrates, characterized by pleiotropic regulatory functions and have been found to discriminate colorectal tumors from normal colorectal mucosa. In the current study, we investigated the prognostic utility of 5′-tiRNA-ProTGG levels in CRC. For this purpose, total RNA was extracted from 155 malignant colorectal tumors and 74 adjacent non-cancerous tissue specimens, polyadenylated and reverse-transcribed using an oligo-dT adapter as primer. Real-time quantitative PCR (qPCR) was used to assess the levels of 5′-tiRNA-ProTGG. Kaplan-Meier survival analysis demonstrated that high 5′-tiRNA-ProTGG levels predict both poor disease-free survival (DFS) and overall survival (OS) of CRC patients. Of note, high 5′-tiRNA-ProTGG levels retain their unfavorable prognostic value in patients with rectal cancer and/or moderately differentiated CRC (grade II). More importantly, multivariate cox regression analysis highlighted that the overexpression of 5′-tiRNA-ProTGG constitutes an adverse prognostic factor predicting short-term relapse of CRC patients independently of the established prognosticators in CRC. Finally, bioinformatics analysis unveiled a potentially critical role of 5′-tiRNA-ProTGG regarding the maintenance of cellular homeostasis, signaling, cell communication, and cellular motility.
Breast Milk from Non-Obese Women with a High Omega-6 to Omega-3 Fatty Acid Ratio, but Not from Women with Obesity, Increases Lipogenic Gene Expression in 3T3-L1 Preadipocytes, Suggesting Adipocyte Dysfunction
Authors: Isesele Peter , Enstad Samantha , Huong Pham , Thomas Raymond , Wagner Carol L. , Sen Sarbattama , Cheema Sukhinder K.
Published: May 2022

Maternal body mass index is associated with breast milk (BM) fatty acid composition. This study investigated the effects of BM omega (n)-6:n-3 polyunsaturated fatty acids (PUFAs) from non-obese women and women with obesity on the process of adipogenesis in 3T3-L1 preadipocytes. BM samples were collected from non-obese women (BMNO) and women with obesity (BMO) at one month postpartum. The fatty acid composition was measured, and BMNO and BMO groups with the lowest (Q1) and highest (Q4) quartiles of n-6:n-3 PUFA ratios were identified. 3T3-L1 preadipocytes were differentiated in the presence or absence of BM. Lipid accumulation and the expression of genes involved in lipogenesis and lipolysis were measured. Treatment with BMNO containing high (vs. low) n-6:n-3 PUFA ratios significantly increased the mRNA expression of lipogenic genes (acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase); however, there was no effect when cells were treated with BMO (with either low or high n-6:n-3 PUFA ratios). Treatment with BMO (high n-6:n-3 PUFA ratio) caused larger lipid droplets. Our findings demonstrated that BMNO with a high n-6:n-3 PUFA ratio was associated with a higher expression of lipogenic genes, while BMO with a high n-6:n-3 PUFA ratio showed larger lipid droplets, suggesting adipocyte dysfunction. These findings may have implications in the BM-mediated programming of childhood obesity.
Immune Checkpoint Blockades in Triple-Negative Breast Cancer: Current State and Molecular Mechanisms of Resistance
Authors: Kim Hyungjoo , Choi Je-Min , Lee Kyung-min
Published: May 2022

Immune checkpoint blockades (ICBs) have revolutionized cancer treatment. Recent studies have revealed a subset of triple-negative breast cancer (TNBC) to be considered as an immunogenic breast cancer subtype. Characteristics of TNBC, such as higher mutation rates and number of tumor-infiltrating immune cells, render the immunogenic phenotypes. Consequently, TNBCs have shown durable responses to ICBs such as atezolizumab and pembrolizumab in clinic. However, a significant number of TNBC patients do not benefit from these therapies, and mechanisms of resistance are poorly understood. Here, we review biomarkers that predict the responsiveness of TNBCs to ICB and recent advances in delineating molecular mechanisms of resistance to ICBs.
Utility of BIM-CFD Integration in the Design and Performance Analysis for Buildings and Infrastructures of Architecture, Engineering and Construction Industry
Authors: Kang Ki-Yeob , Wang Xiangyu , Wang Jun , Xu Shuyuan , Shou Wenchi , Sun Yanhui
Published: May 2022

To scrutinize the current application of building information modelling (BIM) and computational fluid dynamics (CFD) integration in research as well as industrial fields, the present study conducted a holistic review including a bibliometric exploration for existing articles, specific content analysis in different sectors, and follow-up qualitative discussion for the potential of this integrated technology. The bibliometric exploration is focused on analyzing main journals, keywords, and chronological change in representative research content by selecting 115 relevant studies. In content analysis, the representative integrated BIM and CFD application cases are divided into three different sectors. The functionality, interoperability, and sustainability of such integration in architecture, engineering, and construction (AEC) projects are described in detail. Furthermore, the future research based on the applications of BIM and CFD integration is discussed. Specifically, the more advanced hazard analysis is proposed reflecting the strength of such an integration. Comprehensive information for the possible hazards in AEC projects is digitized and quantified to make a more sensitive hazard recognition tool which can formalize reduction strategies and measures of potential hazards. As a result, the present review study contributes to relevant research by identifying representative application parts and practical requirements for BIM and CFD integration in whole design aspects, reviewing the current research trends and future direction in detail, and analyzing the major issues, such as an interoperability in BIM-compatible CFD for sustainable built environments.
Failure Modes of RC Structural Elements and Masonry Members Retrofitted with Fabric-Reinforced Cementitious Matrix (FRCM) System: A Review
Authors: Irandegani Mohammad Ali , Zhang Daxu , Shadabfar Mahdi , Kontoni Denise-Penelope N. , Iqbal Mudassir
Published: May 2022

Much research has been conducted and published on the examination of the behavior of reinforced steel and concrete structures with a FRP system. Nevertheless, the performance of FRP differs from that of FRCM, particularly at high temperature and ultimate strength. The present study provides a review of previous research on structural elements (viz. beams, columns, arches, slabs, and walls) retrofitted with FRCM systems, taking account of various parameters, such as layers, composite types, configurations, and anchors for controlling or delaying failure modes (FMs). Additionally, this paper discussed the details of different FMs observed during experimental tests, such as crushed concrete or bricks, fiber debonding from substrate materials, slippage, fiber rupture, and telescopic failure for strengthened specimens. Moreover, this paper investigated where and how fractures may develop in structural elements retrofitted with the FRCM system under various retrofit scenarios. To this end, in addition to the review of the relevant literature, a large dataset has been compiled from different (RC) structural elements and masonry members. Next, a relationship is developed between failure modes (FMS) and influential parameters, i.e., the number of layers and the type of composite, based on this dataset. This can be used as a benchmark example in future studies, as there is no such basis available in the literature, to the best of the authors’ knowledge.
Immunotherapy and Microbiota for Targeting of Liver Tumor-Initiating Stem-like Cells
Authors: Machida Keigo , Tahara Stanley M.
Published: May 2022

Cancer contains tumor-initiating stem-like cells (TICs) that are resistant to therapies. Hepatocellular carcinoma (HCC) incidence has increased twice over the past few decades, while the incidence of other cancer types has trended downward globally. Therefore, an understanding of HCC development and therapy resistance mechanisms is needed for this incurable malignancy. This review article describes links between immunotherapies and microbiota in tumor-initiating stem-like cells (TICs), which have stem cell characteristics with self-renewal ability and express pluripotency transcription factors such as NANOG, SOX2, and OCT4. This review discusses (1) how immunotherapies fail and (2) how gut dysbiosis inhibits immunotherapy efficacy. Gut dysbiosis promotes resistance to immunotherapies by breaking gut immune tolerance and activating suppressor immune cells. Unfortunately, this leads to incurable recurrence/metastasis development. Personalized medicine approaches targeting these mechanisms of TIC/metastasis-initiating cells are emerging targets for HCC immunotherapy and microbiota modulation therapy.
SGK1 in Cancer: Biomarker and Drug Target
Authors: Cicenas Jonas , Meskinyte-Kausiliene Edita , Jukna Vigilijus , Rimkus Arnas , Simkus Jokubas , Soderholm Diana
Published: May 2022

Serum- and glucocorticoid-regulated kinases (SGKs) are members of the AGC family of serine/threonine kinases, consisting of three isoforms: SGK1, SGK2, and SGK3. SGK1 was initially cloned as a gene transcriptionally stimulated by serum and glucocorticoids in rat mammary tumor cells. It is upregulated in some cancers and downregulated in others. SGK1 increases tumor cell survival, adhesiveness, invasiveness, motility, and epithelial to mesenchymal transition. It stimulates tumor growth by mechanisms such as activation of K+ channels and Ca2+ channels, Na+/H+ exchanger, amino acid and glucose transporters, downregulation of Foxo3a and p53, and upregulation of β-catenin and NFκB. This chapter focuses on major aspects of SGK1 involvement in cancer, its use as biomarker as well as potential therapeutic target.
Prognostic and Predictive Effects of Tumor and Plasma miR-200c-3p in Locally Advanced and Metastatic Breast Cancer
Authors: Navarro-Manzano Esther , Luengo-Gil Ginés , González-Conejero Rocío , García-Garre Elisa , García-Martínez Elena , García-Torralba Esmeralda , Chaves-Benito Asunción , Vicente Vicente , Ayala de la Peña Francisco
Published: May 2022

While the role of miR-200c in cancer progression has been established, its expression and prognostic role in breast cancer is not completely understood. The predictive role of miR-200c in response to chemotherapy has also been suggested by some studies, but only limited clinical evidence is available. The purpose of this study was to investigate miR-200c-3p in the plasma and primary tumor of BC patients. The study design included two cohorts involving women with locally advanced (LABC) and metastatic breast cancer. Tumor and plasma samples were obtained before and after treatment. We found that miR-200c-3p was significantly higher in the plasma of BC patients compared with the controls. No correlation of age with plasma miR-200c-3p was found for controls or for BC patients. MiR-200c-3p tumor expression was also associated with poor overall survival in LABC patients treated with neoadjuvant chemotherapy, independently of pathological complete response or clinical stage. Our findings suggest that plasmatic miR-200c-3p levels could be useful for BC staging, while the tumor expression of miR-200c-3p might provide further prognostic information beyond residual disease in BC treated with neoadjuvant chemotherapy.
Novel T Follicular Helper-like T-Cell Lymphoma Therapies: From Preclinical Evaluation to Clinical Reality
Authors: Krug Adrien , Tari Gamze , Saidane Aymen , Gaulard Philippe , Ricci Jean-Ehrland , Lemonnier François , Verhoeyen Els
Published: May 2022

The classification of peripheral T-cell lymphomas (PTCL) is constantly changing and contains multiple subtypes. Here, we focus on Tfh-like PTCL, to which angioimmunoblastic T-cell lymphoma (AITL) belongs, according to the last WHO classification. The first-line treatment of these malignancies still relies on chemotherapy but gives very unsatisfying results for these patients. Enormous progress in the last decade in terms of understanding the implicated genetic mutations leading to signaling and epigenetic pathway deregulation in Tfh PTCL allowed the research community to propose new therapeutic approaches. These findings point towards new biomarkers and new therapies, including hypomethylating agents, such as azacytidine, and inhibitors of the TCR-hyperactivating molecules in Tfh PTCL. Additionally, metabolic interference, inhibitors of the NF-κB and PI3K-mTOR pathways and possibly novel immunotherapies, such as antibodies and chimeric antigen receptors (CAR) directed against Tfh malignant T-cell surface markers, are discussed in this review among other new treatment options.
Genetic Alterations in Adult T-Cell Leukemia/Lymphoma: Novel Discoveries with Clinical and Biological Significance
Authors: Sakihama Shugo , Karube Kennosuke
Published: May 2022

Adult T-cell leukemia/lymphoma (ATLL) is a refractory T-cell neoplasm that develops in human T-cell leukemia virus type-I (HTLV-1) carriers. Large-scale comprehensive genomic analyses have uncovered the landscape of genomic alterations of ATLL and have identified several altered genes related to prognosis. The genetic alterations in ATLL are extremely enriched in the T-cell receptor/nuclear factor-κB pathway, suggesting a pivotal role of deregulation in this pathway in the transformation of HTLV-1-infected cells. Recent studies have revealed the process of transformation of HTLV-1-infected cells by analyzing longitudinal samples from HTLV-1 carriers and patients with overt ATLL, an endeavor that might enable earlier ATLL diagnosis. The latest whole-genome sequencing study discovered 11 novel alterations, including CIC long isoform, which had been overlooked in previous studies employing exome sequencing. Our study group performed the targeted sequencing of ATLL in Okinawa, the southernmost island in Japan and an endemic area of HTLV-1, where the comprehensive genetic alterations had never been analyzed. We found associations of genetic alterations with HTLV-1 strains phylogenetically classified based on the tax gene, an etiological virus factor in ATLL. This review summarizes the genetic alterations in ATLL, with a focus on their clinical significance, geographical heterogeneity, and association with HTLV-1 strains.
Chronic Lymphocytic Leukemia Progression Diagnosis with Intrinsic Cellular Patterns via Unsupervised Clustering
Authors: Chen Pingjun , El Hussein Siba , Xing Fuyong , Aminu Muhammad , Kannapiran Aparajith , Hazle John D. , Medeiros L. Jeffrey , Wistuba Ignacio I. , Jaffray David , Khoury Joseph D. , Wu Jia
Published: May 2022

Identifying the progression of chronic lymphocytic leukemia (CLL) to accelerated CLL (aCLL) or transformation to diffuse large B-cell lymphoma (Richter transformation; RT) has significant clinical implications as it prompts a major change in patient management. However, the differentiation between these disease phases may be challenging in routine practice. Unsupervised learning has gained increased attention because of its substantial potential in data intrinsic pattern discovery. Here, we demonstrate that cellular feature engineering, identifying cellular phenotypes via unsupervised clustering, provides the most robust analytic performance in analyzing digitized pathology slides (accuracy = 0.925, AUC = 0.978) when compared to alternative approaches, such as mixed features, supervised features, unsupervised/mixed/supervised feature fusion and selection, as well as patch-based convolutional neural network (CNN) feature extraction. We further validate the reproducibility and robustness of unsupervised feature extraction via stability and repeated splitting analysis, supporting its utility as a diagnostic aid in identifying CLL patients with histologic evidence of disease progression. The outcome of this study serves as proof of principle using an unsupervised machine learning scheme to enhance the diagnostic accuracy of the heterogeneous histology patterns that pathologists might not easily see.
EGFR R521K Polymorphism Is Not a Major Determinant of Clinical Cetuximab Resistance in Head and Neck Cancer
Authors: Cserepes Mihály , Nelhűbel Györgyi A. , Meilinger-Dobra Mónika , Herczeg Adrienn , Türk Dóra , Hegedűs Zita , Svajda Laura , Rásó Erzsébet , Ladányi Andrea , Csikó Kristóf György , Kenessey István , Szöőr Árpád , Vereb György , Remenár Éva , Tóvári József
Published: May 2022

Background: Head and neck squamous cell carcinomas (HNSCCs) are among the most abundant malignancies worldwide. Patients with recurrent/metastatic disease undergo combination chemotherapy containing cetuximab, the monoclonal antibody used against the epidermal growth factor receptor (EGFR). Cetuximab augments the effect of chemotherapy; however, a significant number of patients show therapy resistance. The mechanism of resistance is yet to be unveiled, although extracellular alterations of the receptor have been reported, and their role in cetuximab failure has been proposed. Aims: Here, we investigate possible effects of the multi-exon deletion variant (EGFRvIII), and the single nucleotide polymorphism EGFR R521K on cetuximab efficacy. Results: Our results show that in HNSCC patients, the EGFRvIII allele frequency is under 1%; therefore, it cannot lead to common resistance. EGFR R521K, present in 42% of the patients, is investigated in vitro in four HNSCC cell lines (two wild-type and two heterozygous for EGFR R521K). While no direct effect is found to be related to the EGFR status, cells harboring R521K show a reduced sensitivity in ADCC experiments and in vivo xenograft experiments. However, this preclinical difference is not reflected in the progression-free or overall survival of HNSCC patients. Furthermore, NK cell and macrophage presence in tumors is not related to EGFR R521K. Discussion: Our results suggest that EGFR R521K, unlike reported previously, is unable to cause cetuximab resistance in HNSCC patients; therefore, its screening before therapy selection is not justifiable.
Current and Future Therapies for Pancreatic Ductal Adenocarcinoma
Authors: Sally Áine , McGowan Ryan , Finn Karen , Moran Brian Michael
Published: May 2022

Pancreatic cancer is one of the leading causes of cancer-related death worldwide. This is due to delayed diagnosis and resistance to traditional chemotherapy. Delayed diagnosis is often due to the broad range of non-specific symptoms that are associated with the disease. Resistance to current chemotherapies, such as gemcitabine, develops due to genetic mutations that are either intrinsic or acquired. This has resulted in poor patient prognosis and, therefore, justifies the requirement for new targeted therapies. A synthetic lethality approach, that targets specific loss-of-function mutations in cancer cells, has shown great potential in pancreatic ductal adenocarcinoma (PDAC). Immunotherapies have also yielded promising results in the development of new treatment options, with several currently undergoing clinical trials. The utilisation of monoclonal antibodies, immune checkpoint inhibitors, adoptive cell transfer, and vaccines have shown success in several neoplasms such as breast cancer and B-cell malignancies and, therefore, could hold the same potential in PDAC treatment. These therapeutic strategies could have the potential to be at the forefront of pancreatic cancer therapy in the future. This review focuses on currently approved therapies for PDAC, the challenges associated with them, and future directions of therapy including synthetically lethal approaches, immunotherapy, and current clinical trials.
SO2 Tolerance of Rice Hull Ash Based Fe-Cu Catalysts for Low-Temperature CO-SCR of NO
Authors: Teng Zhaohui , Huang Shan , Li Na , Zhou Qulan
Published: May 2022

Rice husk ash (RHA) has potential as a supporter of catalysts. In this research, we studied the activity and SO2 tolerance of RHA-based Fe-Cu oxide in the reduction of NO by CO. Characterization methods were employed to study the properties of the catalysts and their SO2 tolerance. Activity and SO2 resistance were also tested at different temperatures. We recommend two catalysts with SO2 resistance ability: Fe0.67Cu0.33/RHA (the highest catalytic activity) and Fe0.8Cu0.2/RHA. The NO removal rate hardly changed with the addition of SO2 and was kept at about 100%. However, the CO conversion rate decreased with increasing SO2 at the lower reaction temperatures, which may be due to the formation of sulfites. Fortunately, the deactivation was reversible and can be reduced with an increase in the reaction temperature. The results of our research may help promote the application of CO-SCR.
Pre-Conditioning Methods and Novel Approaches with Mesenchymal Stem Cells Therapy in Cardiovascular Disease
Authors: Matta Anthony , Nader Vanessa , Lebrin Marine , Gross Fabian , Prats Anne-Catherine , Cussac Daniel , Galinier Michel , Roncalli Jerome
Published: May 2022

Transplantation of mesenchymal stem cells (MSCs) in the setting of cardiovascular disease, such as heart failure, cardiomyopathy and ischemic heart disease, has been associated with good clinical outcomes in several trials. A reduction in left ventricular remodeling, myocardial fibrosis and scar size, an improvement in endothelial dysfunction and prolonged cardiomyocytes survival were reported. The regenerative capacity, in addition to the pro-angiogenic, anti-apoptotic and anti-inflammatory effects represent the main target properties of these cells. Herein, we review the different preconditioning methods of MSCs (hypoxia, chemical and pharmacological agents) and the novel approaches (genetically modified MSCs, MSC-derived exosomes and engineered cardiac patches) suggested to optimize the efficacy of MSC therapy.
Targeting Histone Deacetylases in Idiopathic Pulmonary Fibrosis: A Future Therapeutic Option
Authors: Korfei Martina , Mahavadi Poornima , Guenther Andreas
Published: May 2022

Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal lung disease with limited therapeutic options, and there is a huge unmet need for new therapies. A growing body of evidence suggests that the histone deacetylase (HDAC) family of transcriptional corepressors has emerged as crucial mediators of IPF pathogenesis. HDACs deacetylate histones and result in chromatin condensation and epigenetic repression of gene transcription. HDACs also catalyse the deacetylation of many non-histone proteins, including transcription factors, thus also leading to changes in the transcriptome and cellular signalling. Increased HDAC expression is associated with cell proliferation, cell growth and anti-apoptosis and is, thus, a salient feature of many cancers. In IPF, induction and abnormal upregulation of Class I and Class II HDAC enzymes in myofibroblast foci, as well as aberrant bronchiolar epithelium, is an eminent observation, whereas type-II alveolar epithelial cells (AECII) of IPF lungs indicate a significant depletion of many HDACs. We thus suggest that the significant imbalance of HDAC activity in IPF lungs, with a “cancer-like” increase in fibroblastic and bronchial cells versus a lack in AECII, promotes and perpetuates fibrosis. This review focuses on the mechanisms by which Class I and Class II HDACs mediate fibrogenesis and on the mechanisms by which various HDAC inhibitors reverse the deregulated epigenetic responses in IPF, supporting HDAC inhibition as promising IPF therapy.
Comparative Analysis of the Quality of Life in Families with Children or Adolescents Having Congenital versus Acquired Neuropathology
Authors: Morcov Maria V. , Pădure Liliana , Morcov Cristian G. , Mirea Andrada , Ghiță Marian , Onose Gelu
Published: May 2022

Aim: This research aims to determine whether the time of injury (congenital or acquired) affects the quality of life (QOL) in families having a child or adolescent with neurological impairment. Design: Comparative, cross-sectional study. Material and methods: To find out if there are differences in the quality of life domains between these two groups, 66 subjects (31 mothers of patients with congenital disorders and 35 mothers of patients with acquired disorders) completed the PedsQL-Family Impact Module (PedsQL-FIM), the World Health Organization Quality of Life Instrument—Short Form (WHOQOL-BRIEF), and the Cognitive Emotion Regulation Questionnaire (CERQ). Results: Analyzing the PedsQL-FIM dimensions, we found significant differences between groups in terms of emotional functioning, communication, and worry, which favor the congenital group. There are no statistically significant differences between social functioning, cognitive functioning, and daily activities groups. No significant differences between groups when evaluating the WHOQOL-BRIEF’s domains (physical health, psychological health, social relationships, environment) have been found. According to CERQ results, adaptive strategies had higher mean scores in the congenital than in the acquired group. The mean score for maladaptive strategies in the congenital group is higher than that in the acquired one, except for catastrophizing, which is higher for acquired. Conclusion: Our findings show that the mothers of patients with acquired neuropathology have a lower quality of life in the emotional functioning, communication, and worry domains.
Improvement in Gait and Participation in a Child with Angelman Syndrome after Translingual Neurostimulation Associated with Goal-Oriented Therapy: A Case Report
Authors: Gaudin-Drouelle Delphine , Houx Laetitia , Lempereur Mathieu , Brochard Sylvain , Pons Christelle
Published: May 2022

Angelman syndrome is a genetic neurobehavioral syndrome characterized by motor and cognitive developmental delay, with a severe reduction in activity and participation. Treatments are limited and the effects of rehabilitation have not been studied. We report on the progress made by a 7-year-old boy with Angelman syndrome following an innovative synergic intervention involving translingual neurostimulation (TLNS) and goal-oriented rehabilitation to improve gait. The parents were interviewed regarding the child’s abilities and participation level and three-dimensional gait analysis was performed before and after the 4-week intervention (five days per week, 4 h per day) and 6 months later. Spatiotemporal and kinematic gait variables improved considerably at 4 weeks, with a reduction in lower limb agonist-antagonist co-contractions, and a large increase in walking distance (from 500 m to 2 km). The child’s engagement and ability to perform activities of daily living improved, as well as several functions not targeted by the intervention. Six months after cessation of the intervention, improvements were partially sustained. The rapid and considerable improvement in motor ability was likely due to potentiation of the rehabilitation by the TLNS. Further studies are required to understand the mechanisms underlying this effect and to determine if it is generalizable to other children with similar disorders.
The Influence of Boron Carbide on the Mechanical Properties and Bonding Strength of B4C/Nickel 63 Coatings of Brake Disc
Authors: Ramesh Balasubramanian , Elsheikh Ammar , Satishkumar Shanmugam , Shaik Abdul , Djuansjah Joy , Ahmadein Mahmoud , Moustafa Essam , Alsaleh Naser
Published: May 2022

Metal-based ceramic composite laser cladding offers substantial compensations in enhancing brake disc surface characteristics. Laser cladding was utilized to combine B4C powder (10–40%) with Ni 63 powder to make Boron Carbide (B4C)/Nickel 63 composite coatings. For the subsequent experiments, the specimens were ground and polished. Bonding strength, fracture toughness, and residual stress were examined with the B4C content. The fracture morphologies were checked using a scanning electron microscope (SEM). It was observed that the bonding strength of various coatings might approach 175 MPa. Best bonding was observed when the B4C level was between 15% and 30%. The porousness of the coating continuously raised as B4C content increased. The coating’s maximum permeability was 5.6% after the B4C level reached 30%. As the B4C level in the coating grew, the coating’s compression resistance decreased. The bonding strength was within desirable limits, and compression resistance was consistently strong. The material bending strength increased when the B4C materials were reduced below 35%; at this level, the bending strength was highest. The bending strength was covered by the optimal range of bonding strength. Good bonding strength and mechanical characteristics were achieved when B4C content was 20% to 30%. The 20% B4C coating had the smoothest fracture morphologies and the strongest bonding strength, making it the most stable. For the estimation of total matrix deformation and corresponding coating stress on coated brake discs, Ansys software was utilized to create a static structural model.
Fresh-Cut Mangoes: How to Increase Shelf Life by Using Neem Oil Edible Coating
Authors: Passafiume Roberta , Tinebra Ilenia , Gaglio Raimondo , Settanni Luca , Sortino Giuseppe , Allegra Alessio , Farina Vittorio
Published: May 2022

The mango is the most widely cultivated tropical fruit. Fresh-cut mango is very useful, but it is very perishable. The purpose of this study was to highlight the effects of neem oil on fresh-cut mango fruits kept for 9 days at 4 ± 1 °C and 80 ± 5% relative humidity. The neem plant (Azadirachta indica) has numerous antioxidant and antibacterial properties. Despite this, very few studies have been carried out on neem oil added to edible coatings (EC) to retard ripening processes. Two formulations were tested: EC1 (hydroxypropyl methylcellulose + CaCl2) and EC2 (hydroxypropyl methylcellulose + CaCl2 + neem oil), both compared with an untreated sample (control). Physicochemical, microbial, proximate and sensory analyses were carried out. Neem oil reduced loss of firmness and colour, while hydroxypropyl methylcellulose and CaCl2 reduced normal cell degradation (weight loss and soluble solids content). Microbiological investigation showed that the EC2 inhibited the development of the main spoilage bacteria during the entire storage period, prolonging the preservation of fresh-cut fruits. The sensory analysis showed a rapid degradation after 5 d in the control sample, while the EC2 was the best.
Electromagnetic Shielding of Composite Films Based on Graphite, Graphitized Carbon Black and Iron-Oxide
Authors: Khomenko Volodymyr , Butenko Oksana , Chernysh Oksana , Barsukov Viacheslav , Suchea Mirela Petruta , Koudoumas Emmanouel
Published: May 2022

The present work regards the development of paint-like composites based on mixtures of carbon materials with magnetite in polyvinyl butyral matrix, and the investigation of the dependence on the electrical characteristics and the frequency of their electromagnetic shielding properties. It was found that high electromagnetic shielding effectiveness requires not only the presence of a high content of carbon components in the composite, but also the absence of an agglomeration of filler particles. Using these paint-like materials, a shielding effectiveness of up to −35 dB of UHF radiation can be obtained. A combination of fillers based on carbon-graphite materials of different morphology and magnetite was found to enhance shielding efficiency.
Strain Profile in the Subsurface of He-Ion-Irradiated Tungsten Accessed by S-GIXRD
Authors: Huang Wenjie , Sun Meng , Wen Wen , Yang Junfeng , Xie Zhuoming , Liu Rui , Wang Xianping , Wu Xuebang , Fang Qianfeng , Liu Changsong
Published: May 2022

The strain profile in the subsurface of He-ion-irradiated W was figured out by unfolding the synchrotron-grazing incidence X-ray diffraction (S-GIXRD) patterns at different incidence angles. The results show that for 2 × 1021 ions/m2 He2+-irradiated W, in addition to a compressive strain exists in the depths of 0–100 nm due to mechanical polishing, an expansion strain appears in the depth beyond 100 nm owing to irradiation-induced lattice swelling. This work provides a reference for the study of irradiation damage in the subsurface by S-GIXRD.
Structural Defect Impact on Changing Optical Response and Raising Unpredicted Ferromagnetic Behaviour in (111) Preferentially Oriented Nanocrystalline NiO Films
Authors: Ben Gouider Trabelsi Amira , Alkallas Fatemah. H. , Ziouche Aicha , Boukhachem Abdelwaheb , Ghamnia Mostefa , Elhouichet Habib
Published: May 2022

NiO thin films deposed on a glass substrate, “NiO/glass”, are successfully prepared using a spray pyrolysis technique (SPT) at 460 °C and characterized via X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray, Atomic force microscopy (AFM), spectroscopic ellipsometry (SE), Photoluminescence (PL) and diverse electric and magnetic studies. The structural investigation shows that the synthesized films crystallized in a cubic structure with (111) preferential orientation. The NiO layers exhibit a uniform grain of regular sizes with aggregates randomly distributed across their surface. The optical properties of the NiO thin films evidenced a normal optical dispersion as well as good transparency of the NiO films. An unpredicted ferromagnetic aspect was raised due to the high oxygen presence in the synthetized material. A high thermal dependency of the conductivity, as well as a semiconductor behavior of the grown NiO material, is also demonstrated.
Interlayer Coupling and Pressure Engineering in Bilayer MoS2
Authors: Qiao Wei , Sun Hao , Fan Xiaoyue , Jin Meiling , Liu Haiyang , Tang Tianhong , Xiong Lei , Niu Binghui , Li Xiang , Wang Gang
Published: May 2022

Controlling the interlayer coupling by tuning lattice parameters through pressure engineering is an important route for tailoring the optoelectronic properties of two-dimensional materials. In this work, we report a pressure-dependent study on the exciton transitions of bilayer MoS2 exfoliated on a diamond anvil surface. The applied hydrostatic pressure changes from ambient pressure up to 11.05 GPa using a diamond anvil cell device. Raman, photoluminescence, and reflectivity spectra at room temperature are analyzed to characterize the interlayer coupling of this bilayer system. With the increase of pressure, the indirect exciton emission disappears completely at about 5 GPa. Importantly, we clearly observed the interlayer exciton from the reflectivity spectra, which becomes invisible at a low pressure around 1.26 GPa. This indicates that the interlayer exciton is very sensitive to the hydrostatic pressure due to the oscillator strength transfer from the direct transition to the indirect one.
Outcomes of Intercalary Endoprostheses as a Treatment for Metastases in the Femoral and Humeral Diaphysis
Authors: Mahdal Michal , Pazourek Lukáš , Apostolopoulos Vasileios , Adámková Krákorová Dagmar , Staniczková Zambo Iva , Tomáš Tomáš
Published: May 2022

Background: The purpose of this study was to evaluate the implant survival, functional score and complications of intercalary endoprostheses implanted for metastatic involvement of the femoral and humeral diaphysis. Methods: The selected group covered patients with bone metastasis who were surgically treated with an intercalary endoprosthesis between 2012 and 2021. The functional outcome was evaluated with the Musculoskeletal Tumor Society (MSTS) scoring system, and complications were evaluated by using the failure classification for prosthetics designed by Henderson. Results: The mean follow-up was 29.8 months. In our group of 25 patients with 27 intercalary endoprostheses (18 femurs, 9 humeri), there were 7 implant-related complications (25.9%), which were more common on the humerus (4 cases, 44.4%) than on the femur (3 cases, 16.7%). Only type II failure—aseptic loosening (5 cases, 18.5%)—and type III failure—structural failure (2 cases, 7.4%)—occurred. There was a significantly higher risk of aseptic loosening of the endoprosthesis in the humerus compared with that in the femur (odds ratio 13.79, 95% confidence interval 1.22–151.05, p = 0.0297). The overall cumulative implant survival was 92% 1 year after surgery and 72% 5 years after surgery. The average MSTS score was 82%. The MSTS score was significantly lower (p = 0.008) in the humerus (75.9%) than in the femur (84.8%). Conclusions: The resection of bone metastases and replacement with intercalary endoprosthesis has excellent immediate functional results with an acceptable level of complications in prognostically favourable patients.
Efficacy of Osimertinib in Lung Squamous Cell Carcinoma Patients with EGFR Gene Mutation–Case Report and a Literature Review
Authors: Rekowska Anna , Rola Piotr , Wójcik-Superczyńska Magdalena , Chmielewska Izabela , Krawczyk Paweł , Milanowski Janusz
Published: May 2022

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer and the leading cause of cancer-related mortality worldwide. It is responsible for 80–85% of lung cancer cases. NSCLC can be divided into several groups, led by adenocarcinoma (ADC)–40–50% and squamous cell carcinoma (SCC)–20–30%. The development of new molecular therapies targeting particular abnormalities such as mutations in the EGFR (Epidermal Growth Factor Receptor) gene or ROS1 or ALK genes rearrangements resolved in novel strategies in advanced NSCLC management. EGFR mutation occurs mostly in patients with ADC and those patients are mostly females with no or light smoking history. The hereby presented patient fitted the ADC characteristics, while they were diagnosed with SCC. The unusual diagnosis implied further genetic testing, which established the occurrence of L858R substitution in exon 21 in the EGFR gene. A 63-year-old female was admitted to the unit due to a dry cough, pain in the right chest area and dyspnoea. When diagnosed, the patient had a peripheral mass in the right lung superior lobe (55 × 40 mm), satellite nodules in the apex of the same lung and packets of disintegrating lymph nodes. Positron Emission Tomography (PET-CT) confirmed a diffuse neoplastic process qualified as stage IV on the TNM scale. Due to EGFR gene mutation, the woman was administered osimertinib, however, the treatment did not succeed, and other therapeutic solutions were undertaken. The patient died 10 months after diagnosis. Patients with advanced ADC harboring EGFR mutation can receive osimertinib, a third-generation tyrosine kinase inhibitor (TKI), however, the use of TKIs in SCC remains controversial. In some published cases, osimertinib treatment led to success, in others, the therapy did not result in the expected final effect. Small sample groups and diverse molecular backgrounds indicate the need for further research in this field. Thus, the treatment decision-making process in those patients overall remains extremely demanding and ambiguous.
Shopping for Ecological Indices? On the Use of Incidence-Based Species Compositional Similarity Measures
Authors: MacGregor-Fors Ian , Escobar Federico , Escobar-Ibáñez Juan F. , Mesa-Sierra Natalia , Alvarado Fredy , Rueda-Hernández Rafael , Moreno Claudia E. , Falfán Ina , Corro Erick J. , Pineda Eduardo , Bourg Amandine , Aguilar-López José L. , Dáttilo Wesley
Published: May 2022

β-diversity has been under continuous debate, with a current need to better understand the way in which a new wave of measures work. We assessed the results of 12 incidence-based β-diversity indices. Our results of gradual species composition overlap between paired assemblages considering progressive differences in species richness show the following: (i) four indices (β-2, β-3, β-3.s, and βr) should be used cautiously given that results with no shared species retrieve results that could be misinterpreted; (ii) all measures conceived specifically as partitioned components of species compositional dissimilarities ought to be used as such and not as independent measures per se; (iii) the non-linear response of some indices to gradual species composition overlap should be interpreted carefully, and further analysis using their results as dependent variables should be performed cautiously; and (iv) two metrics (βsim and βsor) behave predictably and linearly to gradual species composition overlap. We encourage ecologists using measures of β-diversity to fully understand their mathematical nature and type of results under the scenario to be used in order to avoid inappropriate and misleading inferences.
Evaluation and Comparison of Genomic DNA Extraction Methods and PCR Optimization on Archival Formalin-Fixed and Paraffin-Embedded Tissues of Oral Squamous Cell Carcinoma
Authors: Khan Samar Saeed , Tijare Manisha , Kasetty Sowmya , Jain Megha , Alamoudi Ahmed , Bahammam Hammam Ahmed , Bahammam Sarah Ahmed , Bahammam Maha A. , Varadarajan Saranya , Raj A. Thirumal , Patil Shankargouda
Published: May 2022

Recovery and amplification of nucleic acids from archived formalin-fixed tissue samples is the most developing field in retrospective genetic studies. We compared different deparaffinization methods and DNA isolation techniques, and intergroup comparisons were performed to evaluate the effectiveness of different storing methods for archival OSCC samples based on obtained mean DNA quantity, quality, and PCR amplification of the P53 gene. The study comprised 75 archival histologically diagnosed OSCC samples which were divided into Group I: Formalin-fixed paraffin-embedded tissue blocks and Group II: Long-term formalin-fixed tissue. A comparison of different deparaffinization methods showed that xylene deparaffinization is an efficient method to obtain suitable DNA. Comparing different DNA isolation techniques illustrated that the conventional phenol–chloroform method gives better integrity to DNA in contrast with the kit method. Comparison between FFPET and long-term FFT samples demonstrated that samples fixed in formalin overnight and embedded in wax yield better quality and quantity DNA in comparison with long-term samples fixed in formalin. To obtain suitable integrity of DNA, tissue samples should be stored by fixing in formalin overnight followed by preparation of paraffin tissue blocks, deparaffinization by xylene, and subjecting them to the conventional phenol–chloroform DNA isolation protocol.
Asymptotic Normality for Plug-In Estimators of Generalized Shannon’s Entropy
Authors: Zhang Jialin , Shi Jingyi
Published: May 2022

Shannon’s entropy is one of the building blocks of information theory and an essential aspect of Machine Learning (ML) methods (e.g., Random Forests). Yet, it is only finitely defined for distributions with fast decaying tails on a countable alphabet. The unboundedness of Shannon’s entropy over the general class of all distributions on an alphabet prevents its potential utility from being fully realized. To fill the void in the foundation of information theory, Zhang (2020) proposed generalized Shannon’s entropy, which is finitely defined everywhere. The plug-in estimator, adopted in almost all entropy-based ML method packages, is one of the most popular approaches to estimating Shannon’s entropy. The asymptotic distribution for Shannon’s entropy’s plug-in estimator was well studied in the existing literature. This paper studies the asymptotic properties for the plug-in estimator of generalized Shannon’s entropy on countable alphabets. The developed asymptotic properties require no assumptions on the original distribution. The proposed asymptotic properties allow for interval estimation and statistical tests with generalized Shannon’s entropy.
Feature Importance in Gradient Boosting Trees with Cross-Validation Feature Selection
Authors: Adler Afek Ilay , Painsky Amichai
Published: May 2022

Gradient Boosting Machines (GBM) are among the go-to algorithms on tabular data, which produce state-of-the-art results in many prediction tasks. Despite its popularity, the GBM framework suffers from a fundamental flaw in its base learners. Specifically, most implementations utilize decision trees that are typically biased towards categorical variables with large cardinalities. The effect of this bias was extensively studied over the years, mostly in terms of predictive performance. In this work, we extend the scope and study the effect of biased base learners on GBM feature importance (FI) measures. We demonstrate that although these implementation demonstrate highly competitive predictive performance, they still, surprisingly, suffer from bias in FI. By utilizing cross-validated (CV) unbiased base learners, we fix this flaw at a relatively low computational cost. We demonstrate the suggested framework in a variety of synthetic and real-world setups, showing a significant improvement in all GBM FI measures while maintaining relatively the same level of prediction accuracy.