bluuebunny/crossref_metadata_2025 huggingface dataset:
Dataset: Fauna of Adult Ground Beetles (Coleoptera, Carabidae) of the National Park “Smolny” (Russia)
Authors: Ruchin Alexander B. , Alekseev Sergei K. , Artaev Oleg N. , Khapugin Anatoliy A. , Lobachev Evgeniy A. , Lukiyanov Sergei V. , Semishin Gennadiy B.
Published: June 2022

(1) Background: Protected areas are “hotspots” of biodiversity in many countries. In such areas, ecological systems are preserved in their natural state, which allows them to protect animal populations. In several protected areas, the Coleoptera biodiversity is studied as an integral part of the ecological monitoring of the ecosystem state. This study was aimed to describe the Carabidae fauna in one of the largest protected areas of European Russia, namely National Park “Smolny”. (2) Methods: The study was conducted in April–September 2008, 2009, 2017–2021. A variety of ways was used to collect beetles (by hand, caught in light traps, pitfall traps, and others). Seasonal dynamics of the beetle abundance were studied in various biotopes. Coordinates were fixed for each observation. (3) Results: The dataset contains 1994 occurrences. In total, 32,464 specimens of Carabidae have been studied. The dataset contains information about 131 species of Carabidae beetles. In this study, we have not found two species (Carabus estreicheri and Calathus ambiguus), previously reported in the fauna of National Park “Smolny”. (4) Conclusions: The Carabidae diversity in the National Park “Smolny” is represented by 133 species from 10 subfamilies. Ten species (Carabus cancellatus, Harpalus laevipes, Carabus hortensis, Pterostichus niger, Poecilus versicolor, Pterostichus melanarius, Carabus glabratus, Carabus granulatus, Carabus arvensis baschkiricus, Pterostichus oblongopunctatus) constitute the majority of the Carabidae fauna. Seasonal dynamics are maximal in spring; the number of ground beetles decreases in biotopes by autumn.
Hyperparameter Tuning and Automatic Image Augmentation for Deep Learning-Based Angle Classification on Intraoral Photographs—A Retrospective Study
Authors: Cejudo Grano de Oro José Eduardo , Koch Petra Julia , Krois Joachim , Garcia Cantu Ros Anselmo , Patel Jay , Meyer-Lueckel Hendrik , Schwendicke Falk
Published: June 2022

We aimed to assess the effects of hyperparameter tuning and automatic image augmentation for deep learning-based classification of orthodontic photographs along the Angle classes. Our dataset consisted of 605 images of Angle class I, 1038 images of class II, and 408 images of class III. We trained ResNet architectures for classification of different combinations of learning rate and batch size. For the best combination, we compared the performance of models trained with and without automatic augmentation using 10-fold cross-validation. We used GradCAM to increase explainability, which can provide heat maps containing the salient areas relevant for the classification. The best combination of hyperparameters yielded a model with an accuracy of 0.63–0.64, F1-score 0.61–0.62, sensitivity 0.59–0.65, and specificity 0.80–0.81. For all metrics, it was apparent that there was an ideal corridor of batch size and learning rate combinations; smaller learning rates were associated with higher classification performance. Overall, the performance was highest for learning rates of around 1–3 × 10−6 and a batch size of eight, respectively. Additional automatic augmentation improved all metrics by 5–10% for all metrics. Misclassifications were most common between Angle classes I and II. GradCAM showed that the models employed features relevant for human classification, too. The choice of hyperparameters drastically affected the performance of deep learning models in orthodontics, and automatic image augmentation resulted in further improvements. Our models managed to classify the dental sagittal occlusion along Angle classes based on digital intraoral photos.
Automatic Facial Palsy Diagnosis as a Classification Problem Using Regional Information Extracted from a Photograph
Authors: Parra-Dominguez Gemma S. , Garcia-Capulin Carlos H. , Sanchez-Yanez Raul E.
Published: June 2022

The incapability to move the facial muscles is known as facial palsy, and it affects various abilities of the patient, for example, performing facial expressions. Recently, automatic approaches aiming to diagnose facial palsy using images and machine learning algorithms have emerged, focusing on providing an objective evaluation of the paralysis severity. This research proposes an approach to analyze and assess the lesion severity as a classification problem with three levels: healthy, slight, and strong palsy. The method explores the use of regional information, meaning that only certain areas of the face are of interest. Experiments carrying on multi-class classification tasks are performed using four different classifiers to validate a set of proposed hand-crafted features. After a set of experiments using this methodology on available image databases, great results are revealed (up to 95.61% of correct detection of palsy patients and 95.58% of correct assessment of the severity level). This perspective leads us to believe that the analysis of facial paralysis is possible with partial occlusions if face detection is accomplished and facial features are obtained adequately. The results also show that our methodology is suited to operate with other databases while attaining high performance, even though the image conditions are different and the participants do not perform equivalent facial expressions.
Photodynamic Diagnosis Using 5-Aminolevulinic Acid with a Novel Compact System and Chromaticity Analysis for the Detection of Oral Cancer and High-Risk Potentially Malignant Oral Disorders
Authors: Tatehara Seiko , Sato Toru , Takebe Yusuke , Fujinaga Momoka , Tsutsumi-Arai Chiaki , Ito Yumi , Satomura Kazuhito
Published: June 2022

Detecting early-stage oral cancer and precancerous lesions are critical to improving patient prognosis and quality of life after treatment. Photodynamic diagnosis using 5-aminolevulinic acid enables the detection of malignant lesions. This study aimed to improve the diagnostic accuracy of photodynamic diagnosis using an objective chromaticity analysis of fluorescence emitted from oral lesions. Sixty-seven patients with clinically suspicious oral cavity lesions underwent photodynamic diagnosis after topical application of 5-aminolevulinic acid solution, followed by imaging and histological evaluation of the lesions. Chromaticity red and green values were measured from the fluorescence images on the lesion, and the red-to-green ratio was calculated. The photodynamic diagnosis allowed for the visualization of oral cancer and high-risk dysplasia as red fluorescence. Compared to low-risk dysplasia and benign lesions, oral cancer and high-risk dysplasia areas had a significantly higher red value and red-to-green ratio. After setting the cutoff value, sensitivity and specificity were 83.3–88.7% and 83.3–83.9%, respectively, when discriminating between oral cancer or high-risk dysplasia and low-risk dysplasia or benign lesions. Photodynamic diagnosis combined with chromaticity analysis may be a valuable diagnostic tool for detecting oral lesions, with a high likelihood of malignant transformation.
Tissue Optical Clearing: State of the Art and Prospects
Authors: Genina Elina A.
Published: June 2022

The term “tissue optical clearing” (TOC) came into use at the end of the 20th century and is associated with the development of methods for controlling tissue scattering properties using the refractive index matching effect [...]
The Role of Game-Based Learning in Experiential Education: Tool Validation, Motivation Assessment, and Outcomes Evaluation among a Sample of Pharmacy Students
Authors: Dabbous Mariam , Kawtharani Anwar , Fahs Iqbal , Hallal Zahraa , Shouman Dina , Akel Marwan , Rahal Mohamad , Sakr Fouad
Published: June 2022

(1) Background: There is a scarcity of data regarding game-based learning (GBL) in experiential pharmacy education; in addition, the impact of educational games on the attainment of intended learning outcomes and students’ motivation to actively learn and practice through non-traditional pedagogical tools are yet to be explored. (2) Methods: This was a prospective quasi-experimental study that introduced GBL into the Pharmacy Practice Experience course of the Bachelor of Pharmacy (BPharm) program at the Lebanese International University in Lebanon. Data collection took place between July and September 2021. The study objectives were to assess the impact of GBL on attaining intended learning outcomes, determine students’ motivation to engage in GBL, and assess the impact of this motivation on the attainment of learning outcomes. (3) Results: GBL was associated with a significantly higher exam average (mean difference = 7.152, p < 0.001). Moreover, an active learning motivation assessment scale (ALMAS) was constructed and validated; it was found to have good reliability as well as high sensitivity and specificity, and it determined a good level of motivation to engage in GBL. Game-based learners with higher motivation scores had significantly higher exam averages compared to those with lower scores (Beta = 0.296, 95% CI 0.110–0.545, p = 0.004). (4) Conclusion: GBL was associated with better attainment of intended learning outcomes. Students appear to be motivated to learn by this style of active learning, and motivation is prognostic of the attainment of learning outcomes.
An Extendable Software Architecture for Mitigating ARP Spoofing-Based Attacks in SDN Data Plane Layer
Authors: Buzura Sorin , Lehene Mihaiela , Iancu Bogdan , Dadarlat Vasile
Published: June 2022

Software-defined networking (SDN) is an emerging network architecture that brings benefits in network function virtualization, performance, and scalability. However, the scalability feature also increases the number of possible vulnerabilities through multiple entry points in the network. Address Resolution Protocol (ARP) spoofing-based attacks are widely encountered and allow an attacker to assume the identity of a different computer, facilitating other attacks, such as Man in the Middle (MitM). In the SDN context, most solutions employ a controller to detect and mitigate attacks. However, interacting with the control plane involves asynchronous network communication, which causes delayed responses to an attack. The current work avoids these delays by being implemented solely in the data plane through extendable and customizable software architecture. Therefore, faster response times improve network reliability by automatically blocking attackers. As attacks can be generated with a variety of tools and in networks experiencing different traffic patterns, the current solution is created to allow flexibility and extensibility, which can be adapted depending on the running environment. Experiments were run performing ARP spoofing-based attacks using KaliLinux, Mininet, and OpenVSwitch. The presented results are based on traffic pattern analysis offering greater customization capabilities and insight compared to similar work in this area.
Development of a Microalgae-Based Continuous Starch-to-Hydrogen Conversion Approach
Authors: Hupp Bettina , Pap Bernadett , Farkas Attila , Maróti Gergely
Published: June 2022

Eukaryotic algae represent a highly heterogeneous group in terms of organization, lifestyle, and metabolic capabilities. Unicellular green microalgae are capable of biohydrogen production through direct and indirect photolysis as well as dark fermentation. Most algae hydrogen studies focus on axenic algal cultures, although these are difficult and expensive to maintain for continuous operation. Moreover, the complex interplays and metabolic fluxes between algae and bacteria in natural ecosystems provide a number of clear biological and technological benefits to large-scale functional algae-based systems. Two green algae species from the Chlamydomonas and Chlorella genera were used to engineer stable synthetic communities by incorporating a starch-degrading bacterium from the Bacillus genus into the inter-kingdom consortium. Continuous photoheterotrophic biohydrogen production was achieved by elaborating an appropriate algal–bacterial ratio and fine-tuning the culture conditions for the synthetic consortia. Medium with starch as only carbon source served as a simple model of cheap substrate for algal hydrogen generation. The engineered pairwise algal–bacterial associations showed increased biomass and biohydrogen yield compared to the axenic control conditions. Chlorella sp. MACC-360 produced a significantly higher amount of hydrogen when both the bacterium partner and starch were added to the media compared to the axenic algae. Continuous, elevated algal hydrogen production was achieved in media supplemented with 8 g L−1 starch as sole carbon source when carefully selected initial cell number values were used for the Chlorella sp. MACC-360–B. amlyloliquefaciens co-cultures.
Simultaneous Saccharification and Fermentation of Empty Fruit Bunches of Palm for Bioethanol Production Using a Microbial Consortium of S. cerevisiae and T. harzianum
Authors: Derman Eryati , Abdulla Rahmath , Marbawi Hartinie , Sabullah Mohd Khalizan , Gansau Jualang Azlan , Ravindra Pogaku
Published: June 2022

A simultaneous saccharification and fermentation (SSF) optimization process was carried out on pretreated empty fruit bunches (EFBs) by employing the Response Surface Methodology (RSM). EFBs were treated using sequential acid-alkali pretreatment and analyzed physically by a scanning electron microscope (SEM). The findings revealed that the pretreatment had changed the morphology and the EFBs’ structure. Then, the optimum combination of enzymes and microbes for bioethanol production was screened. Results showed that the combination of S. cerevisiae and T. harzianum and enzymes (cellulase and β-glucosidase) produced the highest bioethanol concentration with 11.76 g/L and a bioethanol yield of 0.29 g/g EFB using 4% (w/v) treated EFBs at 30 °C for 72 h. Next, the central composite design (CCD) of RSM was employed to optimize the SSF parameters of fermentation time, temperature, pH, and inoculum concentration for higher yield. The analysis of optimization by CCD predicted that 9.72 g/L of bioethanol (0.46 g/g ethanol yield, 90.63% conversion efficiency) could be obtained at 72 h, 30 °C, pH 4.8, and 6.79% (v/v) of inoculum concentration using 2% (w/v) treated EFBs. Results showed that the fermentation process conducted using the optimized conditions produced 9.65 g/L of bioethanol, 0.46 g/g ethanol yield, and 89.56% conversion efficiency, which was in close proximity to the predicted CCD model.
Human Fire Use and Management: A Global Database of Anthropogenic Fire Impacts for Modelling
Authors: Millington James D. A. , Perkins Oliver , Smith Cathy
Published: June 2022

Human use and management of fire in landscapes have a long history and vary globally in purpose and impact. Existing local research on how people use and manage fire is fragmented across multiple disciplines and is diverse in methods of data collection and analysis. If progress is to be made on systematic understanding of human fire use and management globally, so that it might be better represented in dynamic global vegetation models, for example, we need improved synthesis of existing local research and literature. The database of anthropogenic fire impacts (DAFI) presented here is a response to this challenge. We use a conceptual framework that accounts for categorical differences in the land system and socio-economic context of human fire to structure a meta-study for developing the database. From the data collated, we find that our defined anthropogenic fire regimes have distinct quantitative signatures and identify seven main modes of fire use that account for 93% of fire instance records. We describe the underlying rationales of these seven modes of fire use, map their spatial distribution and summarise their quantitative characteristics, providing a new understanding that could become the basis of improved representation of anthropogenic fire in global process-based models. Our analysis highlights the generally small size of human fires (60% of DAFI records for mean size of deliberately started fires are <21 ha) and the need for continuing improvements in methods for observing small fires via remote sensing. Future efforts to model anthropogenic fire should avoid assuming that drivers are uniform globally and will be assisted by aligning remotely sensed data with field-based data and process understanding of human fire use and management.
Effects of Fermentation Temperature and Time on the Color Attributes and Tea Pigments of Yunnan Congou Black Tea
Authors: Zhu Jiayi , Wang Jinjin , Yuan Haibo , Ouyang Wen , Li Jia , Hua Jinjie , Jiang Yongwen
Published: June 2022

Yunnan Congou black tea (YCBT) is a typical black tea in China, and is rich in theaflavins (TFs), thearubigins (TRs), and theabrownins (TBs). However, the influence of the fermentation temperature and time on the liquor and appearance color and the correlation between the tea pigments and its color attributes remain unclear. We investigated the effects of the fermentation temperature and time on the color attributes and tea pigments of YCBT. A low fermentation temperature was beneficial to maintain a bright orange-red liquor color and promote the accumulation of TFs and TRs. In contrast, a high temperature gave the liquor a glossy appearance and was beneficial for the formation of TBs. A correlation analysis showed that the 10TFRB index best represented the contribution of tea pigments to the quality of black tea. Moreover, TRs and TBs content prediction models were established based on the liquor L and H values, where the former value can be used as an important index to judge the fermentation process. This study will further enrich the theory of black tea processing chemistry and provide technical support for the precise and directional production of black tea.
Comparison of Oxidative and Physical Stabilities of Conjugated Linoleic Acid Emulsions Stabilized by Glycosylated Whey Protein Hydrolysates via Two Pathways
Authors: Li Meng , Li Jinzhe , Huang Yuxuan , Gantumur Munkh-Amgalan , Bilawal Akhunzada , Qayum Abdul , Jiang Zhanmei
Published: June 2022

The objective of the research was to analyze and compare the oxidative and physical stabilities of conjugated linoleic acid (CLA) emulsions stabilized by two glycosylated hydrolysates (GPP-A and GPP-B) that were formed via two different pathways. This study showed that GPP-A exhibited higher browning intensity and DPPH radical scavenging ability in comparison with GPP-B. Moreover, the CLA emulsion formed by GPP-A exhibited a lower creaming index, average particle size, primary and secondary oxidative products, in comparison with GPP-B-loaded emulsion. However, the GPP-A-loaded emulsion showed a higher absolute potential and fraction of interfacial adsorption than that of the CLA emulsion formed by GPP-B. Therefore, the CLA emulsion formed by GPP-A exhibited stronger stabilities in comparison with the GPP-B-loaded emulsion. These results suggested that GPP-A showed an emulsification-based delivery system for embedding CLA to avoid the loss of biological activities. Additionally, the development of CLA emulsions could exert its physiological functions and prevent its oxidation.
Multiple Approaches to Improve the Quality of Cereal-Based Foods
Authors: Laddomada Barbara , Wang Weiqun
Published: June 2022

The interest in improving the health benefits of cereal foods is continuously increasing [...]
Impact of Industrial Practices on the Microbial and Quality Attributes of Fresh Vacuum-Packed Lamb Joints
Authors: de Alba María , Burgess Catherine M. , Pollard Katie , Perussello Camila , Frías-Celayeta Jesús M. , Walsh Des , Carroll Joan , Crofton Emily , Griffin Carol , Botinestean Cristina , Duffy Geraldine
Published: June 2022

The impact of different industrial practices at lamb export abattoirs in Ireland on the microbial and quality attributes of fresh vacuum-packed (VP) lamb leg joints, including Clean Livestock Policy (CLP), fleece clipping, carcass chilling times and vacuum pack storage, at typical chill and retail display temperatures was investigated. Five separate slaughter batches of lamb (ranging in size from 38 to 60 lambs) were followed at two lamb export plants over a two-year period, accounting for seasonal variation. In general, fleece clipping resulted in significantly lower microbial contamination on the fleece than the use of CLP alone. Lamb from carcasses chilled for 24 h had significantly lower psychrophilic total viable counts and Brochothrix thermosphacta and pseudomonad counts than carcasses chilled for 72 h. Following vacuum-packed (VP) storage of meat from these carcasses at 1.7 ± 1.6 °C for 23 days in the meat plant followed by retail display at 3.9 ± 1.7 °C (up to day 50), the dominant microorganisms were lactic acid bacteria, Br. thermosphacta, Enterobacteriaceae and pseudomonads, and all had reached maximum population density by storage day 34. Aligned with this, after day 34, the quality of the raw meat samples also continued to deteriorate, with off-odours and colour changes developing. While the mean values for cooked meat eating quality attributes did not change significantly over the VP storage period, high variability in many attributes, including off-flavours and off-odours, were noted for lamb meat from all storage times, highlighting inconsistences in lamb quality within and between slaughter batches.
Easy-to-Use Visual Sensing System for Milk Freshness, Sensitized with Acidity-Responsive N-Doped Carbon Quantum Dots
Authors: Hu Xuetao , Zhang Xinai , Li Yanxiao , Shi Jiyong , Huang Xiaowei , Li Zhihua , Zhang Junjun , Li Wenting , Xu Yiwei , Zou Xiaobo
Published: June 2022

This study established a flexible and eye-readable sensing system for the easy-to-use, visual detection of milk freshness, using acidity-responsive N-doped carbon quantum dots (N-CQDs). N-CQDs, rich in amino groups and with characteristic acidity sensitivity, exhibited high relative quantum yields of 25.2% and an optimal emission wavelength of 567 nm. The N-CQDs fluorescence quenching upon the dissociated hydrogen ions (H+) in milk and their reacting with the amino groups produced an excellent linear relation (R2 = 0.996) between the fluorescence intensity and the milk acidity, which indicated that the fluorescence of the N-CQDs was highly correlated with milk freshness. Furthermore, a fluorescence sensor was designed by depositing the N-CQDs on filter-papers and starch-gel films, to provide eye-readable signals under UV light. A fluorescence colorimetric card was developed, based on the decrease in fluorescence brightness as freshness deteriorated. With the advantages of high sensitivity and eye readability, the proposed sensor could detect spoiled milk in advance and without any preprocessing steps, offering a promising method of assessing food safety.
Method Validation, Residues and Dietary Risk Assessment for Procymidone in Green Onion and Garlic Plant
Authors: Li Li , Zhao Tingting , Liu Yu , Liang Hongwu , Shi Kaiwei
Published: June 2022

Procymidone is used as a preventive and curative fungicide to control fungal growth on edible crops and ornamental plants. It is one of the most frequently used pesticides and has a high detection rate, but its residue behaviors remain unclear in green onion and garlic plants (including garlic, garlic chive, and serpent garlic). In this study, the dissipation and terminal residues of procymidone in four matrices were investigated, along with the validation of the method and risk assessment. The analytical method for the target compound was developed using gas chromatography-tandem mass spectrometry (GC-MS/MS), which was preceded by a Florisil cleanup. The linearities of this proposed method for investigating procymidone in green onion, garlic, garlic chive, and serpent garlic were satisfied in the range from 0.010 to 2.5 mg/L with R2 > 0.9985. At the same time, the limits of quantification in the four matrices were 0.020 mg/kg, and the fortified recoveries of procymidone ranged from 86% to 104%, with relative standard deviations of 0.92% to 13%. The dissipation of procymidone in green onion and garlic chive followed first-order kinetics, while the half-lives were less than 8.35 days and 5.73 days, respectively. The terminal residue levels in garlic chive were much higher than those in green onion and serpent garlic because of morphological characteristics. The risk quotients of different Chinese consumer groups to procymidone in green onion, garlic chive, and serpent garlic were in the range from 5.79% to 25.07%, which is comparably acceptable. These data could provide valuable information on safe and reasonable use of procymidone in its increasing applications.
Nanoencapsulation of Thyme Essential Oils: Formulation, Characterization, Storage Stability, and Biological Activity
Authors: Jayari Asma , Donsì Francesco , Ferrari Giovanna , Maaroufi Abderrazak
Published: June 2022

This study aimed to improve the effectiveness of Thymus capitatus and Thymus algeriensis essential oils (EOs), as food preservatives, through their encapsulation in different delivery systems (DSs), namely nanoemulsions and biopolymeric nanoparticles. DSs’ preparation is tailored to enhance not only physical stability but also resulting Eos’ antioxidant and antibacterial activities through different fabrication methods (high-pressure homogenization emulsification or antisolvent precipitation) and using different emulsifiers and stabilizers. DSs are characterized in terms of droplet size distribution, ζ-potential, and stability over time, as well as antioxidant and antibacterial activities of encapsulated EOs. The antioxidant activity was studied by the FRAP assay; the antibacterial activity was evaluated by the well diffusion method. EOs of different compositions were tested, namely two EOs extracted from Thymus capitatus, harvested from Tunisia during different periods of the year (TC1 and TC2), and one EO extracted from Thymus algeriensis (TA). The composition of TC1 was significantly richer in carvacrol than TC2 and TA. The most stable formulation was the zein-based nanoparticles prepared with TC1 and stabilized with maltodextrins, which exhibit droplet size, polydispersity index, ζ-potential, and encapsulation efficiency of 74.7 nm, 0.14, 38.7 mV, and 99.66%, respectively. This formulation led also to an improvement in the resulting antioxidant (60.69 µg/mg vs. 57.67 µg/mg for non-encapsulated TC1) and antibacterial (inhibition diameters varying between 12 and 33 mm vs. a range between 12 and 28 mm for non-encapsulated TC1) activities of EO. This formulation offers a promising option for the effective use of natural antibacterial bioactive molecules in the food industry against pathogenic and spoilage bacteria.
Permeability Prediction of Saturated Geomaterials with Revised Pore–Solid Fractal Model and Critical Path Analysis
Authors: Kou Lei , Li Wuxue , Wu Jujie
Published: June 2022

The revised pore–solid fractal (PSF) model is presented by using the largest inscribed circle-based geometries of squares or cubes to replace the original pore or solid subregions as the new pore or solid phase in porous media. The revised PSF model changes the discrete lacunar pore and solid phases in the original PSF model to integrated. Permeability is an intrinsic property of geomaterials and has broad applications in exploring fluid flow and species transport. Based on the revised PSF model and critical path analysis, a fractal model for predicting the permeability of saturated geomaterials is proposed. The permeability prediction model is verified by comparison with the existing predicted model and the laboratory testing. The results show that the predicted permeabilities match the measured values very well. This work provides a theoretical framework for the revised PSF model and its application in predicting the permeability of geomaterials.
Fractal Description of Rock Fracture Networks Based on the Space Syntax Metric
Authors: Sui Lili , Wang Heyuan , Wu Jinsui , Zhang Jiwei , Yu Jian , Ma Xinyu , Sun Qiji
Published: June 2022

Fractal characteristics and the fractal dimension are widely used in the description and characterization of rock fracture networks. They are important tools for coal mining, oil and gas transportation, and other engineering problems. However, due to the complexity of rock fracture networks and the difficulty in directly applying the limit definition of the fractal dimension, the definition and application of the fractal dimension have become hot topics in related projects. In this paper, the traditional fractal calculation methods were reviewed. Using the traditional fractal theory and the head/tail breaks method, a new fractal dimension quantization model was established as a simple method of fractal calculation. This simple method of fractal calculation was used to calculate the fractal dimensions of three rock fracture networks. Through comparison with the box-counting dimension calculation results, it was verified that the model could calculate the fractal dimension of the fracture length of rock fracture networks, as well as quantify it accurately and effectively. In addition, we found a number of similarities between rock fracture networks and urban road traffic networks in GIS. The application of the space syntax metric to rock fracture networks prevents controversy with respect to the definition of the axis and it showed a good effect. Using the space syntax metric as a parameter can better reflect the space relationship of rock fractures than length. Through the calculation of the fractal dimension of the connection value and control value, it was found that the trend of the length fractal dimension was the same as that of the control value, whereas the fractal dimension of the connection value was the opposite. This further verifies the applicability of the space syntax metric in rock fracture networks.
High-Protein Foods for Dysphagia: Manipulation of Mechanical and Microstructural Properties of Whey Protein Gels Using De-Structured Starch and Salts
Authors: Ang Cai Ling , Goh Kelvin Kim Tha , Lim Kaiyang , Matia-Merino Lara
Published: June 2022

This study focuses on understanding the effect of ionic strength on the mechanical and microstructural properties of novel composite gels containing 13% whey protein isolate (WPI) and 4% de-structured waxy potato starch (DWPS). The DWPS is a physically modified waxy potato starch treated at 140 °C for 30 min under constant shear. Thermodynamic incompatibility between WPI and DWPS was observed upon the addition of NaCl (~75 mM) or CaCl2 (10–75 mM). The combined effects of such thermodynamic incompatibility with the changes in protein connectivity induced by varied ionic strength led to the formation of distinctive gel structures (inhomogeneous self-supporting gels with a liquid centre and weak gels with paste-like consistency) that were different from thermodynamic compatible homogeneous self-supporting gels (pure WPI and WPI + maltodextrin gels). At ≥ 250 mM NaCl, instead of a paste-like texture, a recovered soft and creamy self-supporting gel structure was observed when using DWPS. The ability to generate a range of textures in WPI gelation-based foods by using DWPS under different ionic conditions, is a feasible strategy for formulating high-protein foods for dysphagia—aimed to be either thickened fluids or soft solids. Additionally, this acquired knowledge is also relevant when formulating food gels for 3-D printing.
Validation of a Chromosome 14 Risk Haplotype for Idiopathic Epilepsy in the Belgian Shepherd Dog Found to Be Associated with an Insertion in the RAPGEF5 Gene
Authors: Belanger Janelle M. , Heinonen Tiina , Famula Thomas R. , Mandigers Paul J. J. , Leegwater Peter A. , Hytönen Marjo K. , Lohi Hannes , Oberbauer Anita M.
Published: June 2022

An idiopathic epilepsy (IE) risk haplotype on canine chromosome (CFA) 14 has been reported to interact with the CFA37 common risk haplotype in the Belgian shepherd (BS). Additional IE cases and control dogs were genotyped for the risk haplotypes to validate these previous findings. In the new cohort, the interaction between the two regions significantly elevated IE risk. When the haplotypes were analyzed individually, particular haplotypes on both CFA14 (ACTG) and 37 (GG) were associated with elevated IE risk, though only the CFA37 AA was significantly associated (p < 0.003) with reduced risk in the new cohort. However, the CFA14 ACTG risk was statistically significant when the new and previous cohort data were combined. The frequency of the ACTG haplotype was four-fold higher in BS dogs than in other breeds. Whole genome sequence analysis revealed that a 3-base pair predicted disruptive insertion in the RAPGEF5 gene, which is adjacent to the CFA14 risk haplotype. RAPGEF5 is involved in the Wnt-β-catenin signaling pathway that is crucial for normal brain function. Although this risk variant does not fully predict the likelihood of a BS developing IE, the association with a variant in a candidate gene may provide insight into the genetic control of canine IE.
The First Complete Mitochondrial Genome of Eucrate crenata (Decapoda: Brachyura: Goneplacidae) and Phylogenetic Relationships within Infraorder Brachyura
Authors: Pang Xiaoke , Han Chenglong , Guo Biao , Liu Kefeng , Lin Xiaolong , Lu Xueqiang
Published: June 2022

Characterizing the complete mitochondrial genome (mitogenome) of an organism is useful for genomic studies in taxonomy and evolution. The mitogenomic characteristics of Eucrate crenata (Decapoda: Brachyura: Goneplacidae) have never been studied. The present study decodes the first mitogenome of E. crenata by high-throughput sequencing (HTS). The length of the mitogenome is 15,597 bp, and it contains 13 protein-coding genes, 2 ribosomal RNA genes (rrnS and rrnL), and 22 transfer RNA genes. There are 14 and 23 genes observed on the heavy and light strands, respectively. E. crenata possesses a trnH-cac translocation, with the trnH-cac shifted between trnE-gaa and trnF-ttc instead of the usual location between nad5 and nad4 in decapods. Phylogenetic analyses based on the current dataset of 33 Brachyuran mitogenomes indicate that E. crenata. is closely related to Ashtoret lunaris of Matutidae. The similar codon usage and rearrangements in the two species provide evidence for their close phylogenetic relationship. Positive selection analysis showed that one residue located in cox1 was identified as a positively selected site with high BEB value (>95%), indicating that this gene was under positive selection pressure. This study is the first complete mitogenome record for the family Goneplacidae, and the results obtained may improve the understanding of the phylogeny of Goneplacidae in Brachyura.
Comment on Althobaiti et al. Potential Association between the Use of Anabolic Steroids and COVID-19 Infection. Healthcare 2022, 10, 196
Authors: Muciño-Bermejo María-Jimena
Published: June 2022

Recently, Althobaiti, Y. S. and colleagues [...]
A Survey of Mobile Apps for the Care Management of Patients with Dementia
Authors: Kuo Hsiao-Lun , Chang Chun-Hung , Ma Wei-Fen
Published: June 2022

Objective: Dementia is a progressive neurocognitive disorder that currently affects approximately 50 million people globally and causes a heavy burden for their families and societies. This study analyzed mobile apps for dementia care in different languages and during the COVID-19 pandemic. Methods: We searched PubMed, Cochrane Collaboration Central Register of Con-trolled Clinical Trials, Cochrane Systematic Reviews, Google Play Store, Apple App Store, and Huawei App Store for mobile applications for dementia care. The Mobile Application Rating Scale (MARS) was used to assess the quality of applications. Results: We included 99 apps for dementia care. No significant difference in MARS scores was noted between the two language apps (Overall MARS: English: 3.576 ± 0.580, Chinese: 3.569 ± 0.746, p = 0.962). In the subscale analysis, English apps had higher scores of perceived impact than Chinese apps but these were not significant (2.654 ± 1.372 vs. 2.000 ± 1.057, p = 0.061). (2) Applications during the COVID-19 pandemic had higher MARS scores than those before the COVID-19 pandemic but these were not significant (during the COVID-19 pandemic: 3.722 ± 0.416; before: 3.699 ± 0.615, p = 0.299). In the sub-scale analysis, apps during the COVID-19 pandemic had higher scores of engagement than apps before the COVID-19 pandemic but these were not significant (3.117 ± 0.594 vs. 2.698 ± 0.716, p = 0.068). Conclusions: Our results revealed that there is a minor but nonsignificant difference between different languages and during the COVID-19 pandemic. Further cooperation among dementia professionals, technology experts, and caregivers is warranted to provide evidence-based and user-friendly information to meet the needs of users.
Medical Radiation Safety and COVID-19 Knowledge and Awareness among UAE Residents: A Cross-Sectional Study
Authors: Alhasan Mustafa , Abdelrahman Mostafa , Alomaim Wijdan , Hasaneen Mohammad , Al-Horani Qays , Salih Suliman , Nordin Mohd Nazmi
Published: June 2022

Radiologic examinations are valuable tools in the evaluation of COVID-19. A patient-centered care approach encourages patient involvement in decision-making related to their health management. Therefore, patients should have basic knowledge about their disease and its evaluation tools. Therefore, the purpose of this prospective study is to evaluate the public level of knowledge and awareness regarding COVID-19 and radiation safety in the UAE. Methods: A cross-sectional survey study was conducted using an online questionnaire (Google platform). The data collection instrument contained close-ended questions in both Arabic and English. The questions aimed to collect demographic information and to measure the level of knowledge and awareness of COVID-19 and radiation safety. The questionnaire was distributed online using different social media platforms. Results: A total of 1548 participants have completed the questionnaire; 84% were females and 16% were males. The participants’ average age was 24 years. Sixty-eight percent of the participants showed a high level of awareness of the COVID-19 pandemic, while most of the participants (51%) only showed a low level in the radiation safety awareness section. Factors such as Emirates of residence and passively receiving awareness information were shown to predict knowledge and awareness level. Conclusions: The UAE public was found to have a high level of knowledge and awareness about the COVID-19 disease. However, the same could not be said about radiation safety. More effort should be put towards raising the public’s knowledge and awareness about the risk of radiation in order to enable them to participate actively in decisions regarding the radiologic management of their disease.
Virtual Feedback for Arm Motor Function Rehabilitation after Stroke: A Randomized Controlled Trial
Authors: Salvalaggio Silvia , Kiper Pawel , Pregnolato Giorgia , Baldan Francesca , Agostini Michela , Maistrello Lorenza , Turolla Andrea
Published: June 2022

A single-blind randomized controlled trial was conducted to compare whether the continuous visualization of a virtual teacher, during virtual reality rehabilitation, is more effective than the same treatment provided without a virtual teacher visualization, for the recovery of arm motor function after stroke. Teacher and no-teacher groups received the same amount of virtual reality therapy (i.e., 1 h/d, 5 dd/w, 4 ww) and an additional hour of conventional therapy. In the teacher group, specific feedback (“virtual-teacher”) showing the correct kinematic to be emulated by the patient was always displayed online during exercises. In the no-teacher group patients performed the same exercises, without the virtual-teacher assistance. The primary outcome measure was Fugl-Meyer Upper Extremity after treatment. 124 patients were enrolled and randomized, 62 per group. No differences were observed between the groups, but the same number of patients (χ2 = 0.29, p = 0.59) responded to experimental and control interventions in each group. The results confirm that the manipulation of a single instant feedback does not provide clinical advantages over multimodal feedback for arm rehabilitation after stroke, but combining 40 h conventional therapy and virtual reality provides large effect of intervention (i.e., Cohen’s d 1.14 and 0.92 for the two groups, respectively).
Efficacy of Acupuncture, Intravenous Lidocaine, and Diet in the Management of Patients with Fibromyalgia: A Systematic Review and Network Meta-Analysis
Authors: Almutairi Nawaf Masaad , Hilal Faisal Mohammed , Bashawyah Ahmed , Dammas Fatma Al , Yamak Altinpulluk Ece , Hou Jin-De , Lin Jui-An , Varrassi Giustino , Chang Ke-Vin , Allam Abdallah El-Sayed
Published: June 2022

Introduction: This network meta-analysis aimed to assess the efficacy of acupuncture, intravenous lidocaine, and diet compared with other comparators such as physiotherapy and sham/placebo in fibromyalgia patients. Materials and Methods: We searched Embase, PubMed, Scopus, and Web of Science for relevant studies till September 2021. The included studies were randomized controlled clinical trials. For the network meta-analysis, we used the R software. Results: There were 23 included RCTs. The total sample size was 1409 patients. Compared with the sham/placebo group, the network analysis showed the highest improvement in the quality of life in the acupuncture group standardized mean difference (SMD) = −10.28, 95%-CI [−14.96; −5.59]), and then in the physiotherapy group (SMD = −7.48, 95%-CI [−14.72; −0.23]). For the pain, there was a significant reduction with acupuncture (SMD = −1.69, 95%-CI [−2.48; −0.89]), compared with sham/placebo. Regarding depression, it showed a significant reduction with acupuncture (SMD = −9.64, 95%-CI [−16.13; −3.14]) compared with sham/placebo. Finally, for stiffness, it showed no significant differences in the stiffness between acupuncture (SMD = −8.52, 95%-CI [−20.40; 3.36]), fluoxetine (SMD = −6.52, 95%-CI [−29.65; 16.61]), and physiotherapy (SMD = −4.64, 95%-CI [−22.83; 13.54]) compared with sham/placebo. Conclusions: The acupuncture showed a significant effect in the management of fibromyalgia patients. It reduced pain, depression, and enhanced the quality of life. While physiotherapy showed a significant improvement in the quality of life only. In contrast, intravenous lidocaine and diet showed no significant differences when compared with sham/placebo.
Dialysis, Distress, and Difficult Conversations: Living with a Kidney Transplant
Authors: McKeaveney Clare , Noble Helen , Courtney Aisling , Griffin Sian , Gill Paul , Johnston William , Maxwell Alexander , Teasdale Francesca , Reid Joanne
Published: June 2022

Background: Providing holistic care to kidney patients is important; however, without full consideration of the perspectives of people living with a kidney transplant, the provision of truly ‘holistic healthcare’ cannot be possible. It is imperative to understand patient experiences by including kidney patients in key strategies and future renal service planning. Ignoring these important patient views means that there is a significant risk of inappropriate renal service provision and lack of adequate support, impacting overall health. The aim of this study was to develop an in-depth understanding of the lived experiences of kidney transplant recipients. Methods: A total of 23 participants were recruited between two regional nephrology units within the United Kingdom via clinical gatekeepers. In-depth interviews were undertaken. Interviews were digitally recorded, transcribed verbatim, and subjected to interpretative phenomenological analysis. Results: Two themes emerged: “managing ongoing fears of dialysis, distress, and COVID-19” and “dealing with difficult conversations”. Conclusions: Renal healthcare professionals need to understand more than the biological impact of receiving a kidney transplant. Understanding the holistic and multidomain experiences that these participants experience will help healthcare professionals to recognize the needs of this group and ensure more responsive psychosocial care.
Patterns of Internet Use, and Associations with Loneliness, amongst Middle-Aged and Older Adults during the COVID-19 Pandemic
Authors: Wallinheimo Anna-Stiina , Evans Simon L.
Published: June 2022

Loneliness among older adults is a major societal problem with consequences for health and wellbeing; this has been exacerbated by the coronavirus pandemic. The present study investigated associations between internet use, including frequency and type of use, and loneliness in a large UK sample of middle-aged and older adults, aged 55–75 (n = 3500) from the English Longitudinal Study of Ageing (ELSA) cohort study. Our findings indicated a clear relationship between the frequency of internet use and subjective loneliness. Those who used the internet more than once a day reported feeling less lonely than those who used the internet once a week or less. We also found that those who used the internet for e-mail communication were less lonely. However, individuals indicated higher levels of loneliness when the internet was used for information searches about health. Regarding sociodemographic factors underlying internet usage, less frequent use was seen amongst individuals who lived alone, people who were not employed, who had lower education levels, and lower sociodemographic status. Additionally, gender differences were found in the type of internet use: males report using the internet for e-mail communication more than females, while females’ internet use for health-related information searches was higher than in males. In sum, findings suggest that intervention strategies that promote internet access amongst middle-aged and older people could be useful for tackling loneliness and point to the groups within society that should be the focus of such interventions.
Functional Impairments of Amyloidosis Patients: Physical Therapy Assessment
Authors: Redder Elyse , Zhao Qiuhong , Bumma Naresh , Kahwash Rami , Vallakati Ajay , Campbell Courtney , Parikh Samir , Almaani Salem , Freimer Miriam , Efebera Yvonne , Sharma Nidhi
Published: June 2022

Amyloidosis is a rare, systemic disease that can result in significant functional impairment. Specific guidelines for the rehabilitation assessment of amyloidosis patients have yet to be established. The purpose of this study was to identify functional deficits and assess differences based on disease type, organ involvement, age, and gender of patients with amyloidosis. Materials and Methods: The multidisciplinary Comprehensive Amyloidosis Clinic (CAC) at Ohio State University (OSU) has developed structured assessment guidelines for amyloidosis patients. A retrospective, single-institution review of patients assessed in CAC between December 2017 and April 2020 was performed. Outcome measure data from the Timed Up and Go (TUG), 30 s sit-to-stand, and physical function portion of the SF 36 were gathered by chart review. Comparisons were made between CAC patient scores and normative data. Kruskal–Wallis tests were used to compare scores across the disease types (light chain, transthyretin wild-type, and hereditary variant transthyretin) and the Mann–Whitney U test was used for pairwise comparisons within disease types and cardiac involvement. Linear regression models were used to assess associations between patient characteristics (including age, gender, disease type, and cardiac involvement) and performance scores. Results: Data from sixty-four patients was evaluated. On the 30-s sit-to-stand test, patients with light chain amyloidosis performed 3.32 fewer repetitions than patients with transthyretin wild-type, p = 0.03. Patients with cardiac involvement had 2.55 fewer repetitions than patients without cardiac involvement, p = 0.03. Older patients were found to have slower TUG performance, and a 10-year increase in age was associated with an 11% increase in TUG scores. Conclusions: Findings indicate patients with light chain amyloidosis and patients with cardiac involvement, when compared to other amyloidosis patients, present with more physical impairments.