bluuebunny/crossref_metadata_2025 huggingface dataset:
Formation of Neutrophil Extracellular Traps by Reduction of Cellular Cholesterol Is Independent of Oxygen and HIF-1α
Authors: Henneck Timo , Mergani AhmedElmontaser , Clever Sabrina , Seidler Anna E. , Brogden Graham , Runft Sandra , Baumgärtner Wolfgang , Branitzki-Heinemann Katja , von Köckritz-Blickwede Maren
Published: March 2022

Formation of neutrophil extracellular traps (NETs) is a two-faced innate host defense mechanism, which, on the one hand, can counteract microbial infections, but on the other hand, can contribute to massive detrimental effects on the host. Cholesterol depletion from the cellular membrane by Methyl-β-cyclodextrin (MβCD) is known as one of the processes initiating NET formation. Since neutrophils mainly act in an inflammatory environment with decreased, so-called hypoxic, oxygen conditions, we aimed to study the effect of oxygen and the oxygen stress regulator hypoxia-inducible factor (HIF)-1α on cholesterol-dependent NET formation. Thus, murine bone marrow-derived neutrophils from wild-type and HIF-knockout mice or human neutrophils were stimulated with MβCD under normoxic (21% O2) compared to hypoxic (1% O2) conditions, and the formation of NETs were studied by immunofluorescence microscopy. We found significantly induced NET formation after treatment with MβCD in murine neutrophils derived from wild-type as well as HIF-1α KO mice at both hypoxic (1% O2) as well as normoxic (21% O2) conditions. Similar observations were made in freshly isolated human neutrophils after stimulation with MβCD or statins, which block the HMG-CoA reductase as the key enzyme in the cholesterol metabolism. HPLC was used to confirm the reduction of cholesterol in treated neutrophils. In summary, we were able to show that NET formation via MβCD or statin-treatment is oxygen and HIF-1α independent.
DkmiR397 Regulates Proanthocyanidin Biosynthesis via Negative Modulating DkLAC2 in Chinese PCNA Persimmon
Authors: Zaman Fatima , Zhang Meng , Liu Ying , Wang Zhilin , Xu Liqing , Guo Dayong , Luo Zhengrong , Zhang Qinglin
Published: March 2022

Persimmon fruits accumulate a large amount of proanthocyanidins (PAs), which makes an astringent sensation. Proanthocyanidins (PAs) are the polymers of flavan-3-ols stored in plant vacuoles under laccase activation. A laccase gene, DkLAC2, is putatively involved in PAs biosynthesis and regulated by microRNA (DkmiR397) in persimmon. However, the polymerization of PAs in association with miRNA397 still needs to be explored in persimmon. Here, we identified pre-DkmiR397 and its target gene DkLAC2 in ‘Eshi 1’ persimmon. Histochemical staining with GUS and dual luciferase assay both confirmed DkmiR397-DkLAC2 binding after co-transformation in tobacco leaves. Diverse expression patterns of DkLAC2 and DkmiR397 were exhibited during persimmon fruit development stages. Moreover, a contrasting expression pattern was also observed after the combined DkLAC2-miR397 transformation in persimmon leaves, suggesting that DkmiR397 might be a negative regulator of DkLAC2. Similarly, the transient transformation of DkmiR397 in persimmon fruit discs in vitro also reduced PA accumulation by repressing DkLAC2, whereas the up-regulation of DkLAC2 increased the accumulation of PAs by short tandem target mimic STTM-miR397. A similar expression pattern was observed when overexpressing of DkLAC2 in Arabidopsis wild type (WT) and overexpression of DkLAC2, DkmiR397 in persimmon leaf callus. Our results revealed that the role of DkmiR397 repressed the expression of DkLAC2 concerning PA biosynthesis, providing a potential target for the manipulation of PAs metabolism in persimmon.
OsbZIP18, a Positive Regulator of Serotonin Biosynthesis, Negatively Controls the UV-B Tolerance in Rice
Authors: Sun Yangyang , Wang Bi , Ren Junxia , Zhou Yutong , Han Yu , Niu Shuying , Zhang Yuanyuan , Shi Yuheng , Zhou Junjie , Yang Chenkun , Ma Xuemin , Liu Xianqing , Luo Yuehua , Jin Cheng , Luo Jie
Published: March 2022

Serotonin (5-hydroxytryptamine) plays an important role in many developmental processes and biotic/abiotic stress responses in plants. Although serotonin biosynthetic pathways in plants have been uncovered, knowledge of the mechanisms of serotonin accumulation is still limited, and no regulators have been identified to date. Here, we identified the basic leucine zipper transcription factor OsbZIP18 as a positive regulator of serotonin biosynthesis in rice. Overexpression of OsbZIP18 strongly induced the levels of serotonin and its early precursors (tryptophan and tryptamine), resulting in stunted growth and dark-brown phenotypes. A function analysis showed that OsbZIP18 activated serotonin biosynthesis genes (including tryptophan decarboxylase 1 (OsTDC1), tryptophan decarboxylase 3 (OsTDC3), and tryptamine 5-hydroxylase (OsT5H)) by directly binding to the ACE-containing or G-box cis-elements in their promoters. Furthermore, we demonstrated that OsbZIP18 is induced by UV-B stress, and experiments using UV-B radiation showed that transgenic plants overexpressing OsbZIP18 exhibited UV-B stress-sensitive phenotypes. Besides, exogenous serotonin significantly exacerbates UV-B stress of OsbZIP18_OE plants, suggesting that the excessive accumulation of serotonin may be responsible for the sensitivity of OsbZIP18_OE plants to UV-B stress. Overall, we identified a positive regulator of serotonin biosynthesis and demonstrated that UV-B-stress induced serotonin accumulation, partly in an OsbZIP18-dependent manner.
HNF1A Mutations and Beta Cell Dysfunction in Diabetes
Authors: Miyachi Yasutaka , Miyazawa Takashi , Ogawa Yoshihiro
Published: March 2022

Understanding the genetic factors of diabetes is essential for addressing the global increase in type 2 diabetes. HNF1A mutations cause a monogenic form of diabetes called maturity-onset diabetes of the young (MODY), and HNF1A single-nucleotide polymorphisms are associated with the development of type 2 diabetes. Numerous studies have been conducted, mainly using genetically modified mice, to explore the molecular basis for the development of diabetes caused by HNF1A mutations, and to reveal the roles of HNF1A in multiple organs, including insulin secretion from pancreatic beta cells, lipid metabolism and protein synthesis in the liver, and urinary glucose reabsorption in the kidneys. Recent studies using human stem cells that mimic MODY have provided new insights into beta cell dysfunction. In this article, we discuss the involvement of HNF1A in beta cell dysfunction by reviewing previous studies using genetically modified mice and recent findings in human stem cell-derived beta cells.
The Development and Evaluation of Insect Traps for the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae), Vector of Citrus Huanglongbing
Authors: Snyder James , Dickens Katrina L. , Halbert Susan E. , Dowling Stefanie , Russell Dyrana , Henderson Ruth , Rohrig Eric , Ramadugu Chandrika
Published: March 2022

Citrus huanglongbing (HLB) is a severe problem for citrus cultivation. The disease management programs benefit from improved field tools suitable for surveying the ACP vector (Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae)) and the associated pathogen. In the present study, we utilize three-dimensional (3D) printers and design tools to develop traps that can capture and preserve ACPs. Three novel, 3D-printed traps were designed and evaluated: stem trap, and cylinder traps 1 and 2. The traps and yellow sticky cards were deployed weekly for 8 months in 2 non-commercial citrus groves in Florida; in California, the traps were evaluated for 12 months in field cages and 4 citrus groves. The stem traps captured lower numbers of ACPs at all experimental sites compared to the cylinder traps. Capture rates in the cylinder traps were comparable to the sticky trap, making the device a viable tool for monitoring field ACPs. The two main advantages of using the reusable 3D traps over standard methods of ACP and HLB surveys include dynamic sampling that can be conducted year-round and the capture of ACPs that can be preserved and tested. Improved trapping may facilitate quick management decisions and mitigate HLB.
Clinical, Laboratory and Lung Ultrasound Assessment of Congestion in Patients with Acute Heart Failure
Authors: Palazzuoli Alberto , Evangelista Isabella , Beltrami Matteo , Pirrotta Filippo , Tavera Maria Cristina , Gennari Luigi , Ruocco Gaetano
Published: March 2022

Congestion is the main cause of hospitalization in patients with acute heart failure (AHF), however its precise assessment by simple clinical evaluation remains elusive. The recent introduction of the lung ultrasound scan (LUS) allowed to physicians to more precisely quantify pulmonary congestion. The aim of this study was to compare clinical congestion (CC) with LUS and B-type natriuretic peptide (BNP) in order to achieve a more complete evaluation and to evaluate the prognostic power of each measurement. Methods: All patients were submitted to clinical evaluation for blood sample analysis and LUS at admission and before discharge. LUS protocol evaluated the number of B-lines for each chest zone by standardized eight site protocol. CC was measured following ESC criteria. The mean difference between admission and discharge congestion logBNP and B-lines values were calculated. Combined end points of death and rehospitalization was calculated over 180 days. Results: 213 patients were included in the protocol; 133 experienced heart failure with reduced ejection fraction (HFrEF), and 83 presented with heart failure with preserved ejection fraction (HFpEF). Patients with HFrEF had a more increased level of BNP (1150 (812–1790) vs. 851 (694–1196); p = 0.002) and B lines total number (32 (27–38) vs. 30 (25–36); p = 0.05). A positive correlation was found between log BNP and Blines number in both HFrEF (r = 0.57; p < 0.001) and HFpEF (r = 0.36; p = 0.001). Similarly, dividing B-lines among tertiles the upper group (B-lines ≥ 36) had an increased clinical congestion score. Among three variables at admission only B-lines were predictive for outcome (AUC 0.68 p < 0.001) but not LogBNP and CC score. During 180 days of follow-up, univariate analysis showed that persistent ΔB-lines <−32.3% (HR 6.54 (4.19–10.20); p < 0.001), persistent ΔBNP < −43.8% (HR 2.48 (1.69–3.63); p < 0.001) and persistent ΔCC < 50% (HR 4.25 (2.90–6.21); p < 0.001) were all significantly related to adverse outcome. Multivariable analysis confirmed that persistent ΔB-lines (HR 4.38 (2.64–7.29); p < 0.001), ΔBNP (HR 1.74 (1.11–2.74); p = 0.016) and ΔCC (HR 3.38 (2.10–5.44); p < 0.001 were associated with the combined end point. Conclusions: a complete clinical laboratory and LUS assessment better recognized different congestion occurrence in AHF. The difference between admission and discharge B-lines provides useful prognostic information compared to traditional clinical evaluation.
A Comparative Study of the Efficacy of an Intervention with a Nutritional Supplement for Patients with Chronic Kidney Disease: A Randomized Trial
Authors: Castro-Barquero Sara , Arias-Guillén Marta , Pi-Oriol Sofia , Sacanella Emilio , Romano-Andrioni Barbara , Vidal-Lletjós Sandra , Ruiz-León Ana María , Estruch Ramon , Casas Rosa
Published: March 2022

Chronic kidney disease (CKD) involves heterogeneous diseases that affect the renal structure and function. Malnutrition plays a crucial role during patients with CKD on hemodialysis (HD) treatment and is associated with an increased rate and duration of hospitalizations. The aim of this randomized, parallel, intervention-controlled trial was to assess whether the use of daily supplementation with a new nutritional product developed by the Grand Fontaine Laboratories improves the nutritional status and anthropometric parameters of stage 5 CKD patients, compared with standard renal dietary advice, after three months of follow-up. Dietary intake, anthropometric measurements, physical activity, and blood samples were collected at baseline and after three months of intervention. Significant improvements were observed within the intervention group in body weight (1.5 kg [95% CI: 0.9 to 2.12 kg]) and BMI (0.54 kg/m2 [95% CI: 0.31 to 0.77]; p-value between groups, 0.002 and 0.006, respectively). In the control group, significant decreases were observed in transferrin saturation (−5.04% [95% CI: −8.88 to −1.21]) and alpha-tocopherol levels (−3.31 umol/L [95% CI: −6.30 to −0.32]). We concluded that daily dietary intake of a specific renal nutritional complement in CKD patients with or at risk of malnutrition may prevent deterioration in nutritional parameters.
A Nomogram for Predicting Non-Response to Surgery One Year after Elective Total Hip Replacement
Authors: Dowsey Michelle M. , Spelman Tim , Choong Peter F. M.
Published: March 2022

Background: Total hip replacement (THR) is a common and cost-effective procedure for end-stage osteoarthritis, but inappropriate utilization may be devaluing its true impact. The purpose of this study was to develop and test the internal validity of a prognostic algorithm for predicting the probability of non-response to THR surgery at 1 year. Methods: Analysis of outcome data extracted from an institutional registry of individuals (N = 2177) following elective THR performed between January 2012 and December 2019. OMERACT-OARSI responder criteria were applied to Western Ontario and McMaster Universities Arthritis Index (WOMAC) pain and function scores at pre- and 1 year post-THR, to determine non-response to surgery. Independent prognostic correlates of post-operative non-response observed in adjusted modelling were then used to develop a nomogram. Results: A total of 194 (8.9%) cases were deemed non-responders to THR. The degree of contribution (OR, 95% CI) of each explanatory factor to non-response on the nomogram was, morbid obesity (1.88, 1.16, 3.05), Kellgren–Lawrence grade <4 (1.89, 1.39, 2.56), WOMAC Global rating per 10 units (0.86, 0.79, 0.94) and the following co-morbidities: cerebrovascular disease (2.39, 1.33, 4.30), chronic pulmonary disease (1.64; 1.00, 2.71), connective tissue disease (1.99, 1.17, 3.39), diabetes (1.86, 1.26, 2.75) and liver disease (2.28, 0.99, 5.27). The concordance index for the nomogram was 0.70. Conclusion: We have developed a prognostic nomogram to calculate the probability of non-response to THR surgery. In doing so, we determined that both the probability of and predictive prognostic factors for non-response to THR differed from a previously developed nomogram for total knee replacement (TKR), confirming the benefit of designing decision support tools that are both condition and surgery site specific. Future external validation of the nomogram is required to confirm its generalisability.
Characteristic Assessment of Angiographies at Different Depths with AS-OCTA: Implication for Functions of Post-Trabeculectomy Filtering Bleb
Authors: Luo Man , Zhu Yingting , Xiao Hui , Huang Jingjing , Ling Jin , Huang Haishun , Li Yiqing , Zhuo Yehong
Published: March 2022

This study aimed to analyze the quantitative vascular biomarkers of filtering bleb function at different depths using anterior segment optical coherence tomography angiography (AS-OCTA). This cross-sectional study is registered on Clinicaltrails.gov (NCT 04515017). Forty-six eyes with primary open-angle glaucoma that had undergone trabeculectomy with mitomycin-C for more than six months were included. Vessel density (VD) and vessel diameter index (VDI) in the superficial layer (SL), Tenon’s layer (TL), and deep layer (DL) of the bleb were obtained. The VD and VDI were higher in the failure group (both p = 0.000). Significant correlations were found between the SL, TL, DL’s VDI, and IOP in the success group (p = 0.013, 0.016, 0.031, respectively). The VD of the TL and DL were related to IOP in the failure group (p = 0.012, 0.009). Tenon’s VD (TVD) and Tenon’s VDI (TVDI) correlated with IOP adjusting for TVD, TVDI, and the Indiana Bleb Appearance Grading Scale (IBAGS) (p = 0.009, 0.043) or Kenfeld grading system (KGS) (p = 0.011, 0.016). The area under curve (AUC) of the TVD, TVDI, IBAGS, and KGS to predict surgery failure were 0.960, 0.925, 0.770, and 0.850. AS-OCTA realized the quantitative evaluation of vessels, especially the invisible vascularity beneath the conjunctiva. TVD and TVDI as detected by AS-OCTA better reflected bleb function than conventional grading systems.
An Efficient Method for Nested High-Resolution Ocean Modelling Incorporating a Data Assimilation Technique
Authors: Shapiro Georgy , Gonzalez-Ondina Jose
Published: March 2022

A simple and computationally efficient method is presented for creating a high-resolution regional (child) model nested within a coarse-resolution, good-quality data-assimilating (parent) model. The method, named Nesting with Downscaling and Data Assimilation (NDA), reduces bias and root mean square errors (RMSE) of the child model and does not allow the child model to drift from reality. Usually coarser resolution models, e.g., global scale, are used to provide boundary conditions for the nested child model. The basic idea of the NDA method is to use a complete 3D set of output data from the parent model using a process which is similar to data assimilation of observations into an ocean model. In this way, the child model is physically aware of observations via the parent model. The method allows for avoiding a complex process of assimilating the same observations which were already assimilated into the parent model. The NDA method is illustrated in several simple 2D synthetic cases where the true solution is known. The NDA method reduces the child model bias to the same level as in the parent model and reduces the RMSE, typically by a factor of two to five, occasionally more.
Morel Production Associated with Soil Nitrogen-Fixing and Nitrifying Microorganisms
Authors: Yu Feng-Ming , Jayawardena Ruvishika Shehali , Thongklang Naritsada , Lv Meng-Lan , Zhu Xue-Tai , Zhao Qi
Published: March 2022

True morels (Morchella, Pezizales) cultivated in soil are subject to complex influences from soil microbial communities. To explore the characteristics of soil microbial communities on morel cultivation, and evaluate whether these microbes are related to morel production, we collected 23 soil samples from four counties in Sichuan and Yunnan Provinces, China. Based on ITS and 16S rDNA amplicon sequencing, the alpha diversity analysis indicated that the biodiversity of morel cultivation soil showed a downward trend compared with the bare soil. The results also showed that there were no significant differences in soil microbial communities between OC (bare soil) and OO (after one-year suspension of sowing). This means that, after about one year of stopping sowing, the component and structure of soil that once cultivated morel would be restored. In co-occurrence networks, some noteworthy bacterial microbes involved in nitrogen fixation and nitrification have been identified in soils with high morel yields, such as Arthrobacter, Bradyhizobium, Devosia, Pseudarthrobacter, Pseudolabrys, and Nitrospira. In contrast, in soils with low or no morel yield, some pathogenic fungi accounted for a high proportion, including Gibberella, Microidium, Penicillium, Sarocladium, Streptomyces, and Trichoderma. This study provided valuable information for the isolation and culturing of some beneficial microbes for morel cultivation in further study and, potentially, to harness the power of the microbiome to improve morel production and health.
Sporobolomyces lactucae sp. nov. (Pucciniomycotina, Microbotryomycetes, Sporidiobolales): An Abundant Component of Romaine Lettuce Phylloplanes
Authors: Fatemi Samira , Haelewaters Danny , Urbina Hector , Brown Samuel , Houston Makenna L. , Aime M. Catherine
Published: March 2022

Shifts in food microbiomes may impact the establishment of human pathogens, such as virulent lineages of Escherichia coli, and thus are important to investigate. Foods that are often consumed raw, such as lettuce, are particularly susceptible to such outbreaks. We have previously found that an undescribed Sporobolomyces yeast is an abundant component of the mycobiome of commercial romaine lettuce (Lactuca sativa). Here, we formally describe this species as Sporobolomyces lactucae sp. nov. (Pucciniomycotina, Microbotryomycetes, and Sporidiobolales). We isolated multiple strains of this yeast from commercial romaine lettuce purchased from supermarkets in Illinois and Indiana; additional isolates were obtained from various plant phylloplanes in California. S. lactucae is a red-pigmented species that is similar in appearance to other members of the genus Sporobolomyces. However, it can be differentiated by its ability to assimilate glucuronate and D-glucosamine. Gene genealogical concordance supports S. lactucae as a new species. The phylogenetic reconstruction of a four-locus dataset, comprising the internal transcribed spacer and large ribosomal subunit D1/D2 domain of the ribosomal RNA gene, translation elongation factor 1-α, and cytochrome B, places S. lactucae as a sister to the S. roseus clade. Sporobolomyces lactucae is one of the most common fungi in the lettuce microbiome.
Mechanism of Wheat Leaf Rust Control Using Chitosan Nanoparticles and Salicylic Acid
Authors: Elsharkawy Mohsen , Omara Reda , Mostafa Yasser , Alamri Saad , Hashem Mohamed , Alrumman Sulaiman , Ahmad Abdelmonim
Published: March 2022

Wheat leaf rust is one of the world’s most widespread rusts. The progress of the disease was monitored using two treatments: chitosan nanoparticles and salicylic acid (SA), as well as three application methods; spraying before or after the inoculation by 24 h, and spraying both before and after the inoculation by 24 h. Urediniospore germination was significantly different between the two treatments. Wheat plants tested for latent and incubation periods, pustule size and receptivity and infection type showed significantly reduced leaf rust when compared to untreated plants. Pucciniatriticina urediniospores showed abnormalities, collapse, lysis, and shrinkage as a result of chitosan nanoparticles treatment. The enzymes, peroxidase and catalase, were increased in the activities. In both treatments, superoxide (O2−) and hydrogen peroxide (H2O2), were apparent as purple and brown discolorations. Chitosan nanoparticles and SA treatments resulted in much more discoloration and quantitative measurements than untreated plants. In anatomical examinations, chitosan nanoparticles enhanced thickness of blade (µ), thickness of mesophyll tissue, thickness of the lower and upper epidermis and bundle length and width in the midrib compared to the control. In the control treatment’s top epidermis, several sori and a large number of urediniospores were found. Most anatomical characters of flag leaves in control plants were reduced by biotic stress with P. triticina. Transcription levels of PR1-PR5 and PR10 genes were activated in chitosan nanoparticles treated plants at 0, 1 and 2 days after inoculation. In light of the data, we suggest that the prospective use of chitosan nanoparticles might be an eco-friendly strategy to improve growth and control of leaf rust disease.
A 3-Dimensional Frame of Reference for Prevention of Risk in Supply Chain
Authors: Nguyen Han-Khanh
Published: March 2022

Businesses have to deal with many potential risks in the supply chain, especially during the COVID-19 pandemic. The retail market in Vietnam has great potential for long-term development with the birth and rapid development of domestic supermarkets. However, market opening policies have resulted in fierce competition from a large number of foreign supermarkets. At the same time, customers have become more professional in their approach to shopping and carefully consider any decisions about shopping and the use of services at supermarkets. In this study, the authors use three models (i.e., the SERVQUAL model, the binary logistic model, and the Grey model) corresponding to a three-dimensional frame of reference (i.e., past, present, and future) to provide supermarket managers with a multi-dimensional view of the supermarket business situation. The results identify four factors−namely, quality of goods, personnel, safety, and facilities and equipment−that significantly impact customer satisfaction. The second frame of reference shows that factors such as age, academic level, and income affect the decision to reuse any service at the supermarket. The third frame of reference provides supermarket managers with forecast data about the supermarket business situation for 2021 to 2024. These results provide a solid foundation for supermarket managers seeking to develop strategies and take measures to adjust business activities to achieve the best business efficiency and avoid potential risks in the company’s supply chain. In addition, the results of this study are valuable references for researchers in the fields of customer service, supply chain management, and customer behavior. In particular, the factors obtained in this study will greatly strengthen the scientific value of the service sector and the model of retail supermarkets in Vietnam and other countries around the world. In fact, the business strategy of supermarkets still depends on the spread of COVID-19. Therefore, in the future, it is necessary to combine the results of this study with the experience of managers to develop the right business strategies and achieve further results and sustainable development.
Spatiotemporal Change Analysis and Prediction of Future Land Use and Land Cover Changes Using QGIS MOLUSCE Plugin and Remote Sensing Big Data: A Case Study of Linyi, China
Authors: Muhammad Rizwan , Zhang Wenyin , Abbas Zaheer , Guo Feng , Gwiazdzinski Luc
Published: March 2022

Land use and land cover (LULC) change analysis is a systematic technique that aids in the comprehension of physical and non-physical interaction with the natural habitat and the pursuit of environmental sustainability. Research regarding LULC’s spatiotemporal changing patterns and the simulation of future scenarios offers a complete view of present and future development possibilities. To simulate the spatiotemporal change transition potential and future LULC simulation, we utilized multi-temporal remotely sensed big data from 1990 to 2020 with a 10-year interval. Independent variables (DEM, slope, and distance from roads) and an integrated CA-ANN methodology within the MOLUSCE plugin of QGIS were utilized. The findings reveal that physical and socioeconomic driving variables have a substantial effect on the patterns of the terrain. In the last three decades, the study area had a significant rise in impervious surface from 10.48% to 26.91%, as well as a minor increase in water from 1.30% to 1.67%. As a result, forest cover decreased from 12.60% to 8.74%, green space decreased from 26.34% to 16.57%, and barren land decreased from 49.28% to 46.11%. Additionally, the predictions (2030–2050) support the increasing trend towards impervious surface at the expense of significant quantities of forest and green space.
Impacts of COVID-19 Pandemic on the Global Flows of People and Goods: Implications on the Dynamics of Urban Systems
Authors: Gazzeh Karim , Abubakar Ismaila Rimi , Hammad Emad
Published: March 2022

The emergence of the COVID-19 pandemic has significantly disrupted the flows or spatial mobility of people, goods, and services globally. The present study explored the impact of the pandemic on the global flows of people and goods, and the implications on the dynamics of urban systems. The study utilized desktop research methodology to collect relevant literature and secondary data, which were analyzed using content analysis and descriptive statistics. The study found that the restrictive measures imposed during the pandemic severely disrupted the global flows of people and goods. As a result, global movements of people declined by over 40% in 2020 from the 2019 levels. Similarly, the global flows of goods shrunk by at least 10% within the same period. These lockdown-related disruptions have significant implications on how socioeconomic activities are organized and conducted within and between cities, with civil aviation and hospitality sectors the hardest hit. The study concludes that there is a need for resilient urban systems in which cities, people, institutions, and firms can effectively adapt to the impact of the pandemic.
Intensive Evaluation and High-Quality Redevelopment of Enterprise Land Use: A Case Study in China
Authors: Yang Zhiheng , Li Shaoxing , Sun Dongqi , Li Chenxi , Wu Jiahui
Published: March 2022

The reuse of existing industrial spatial resources in China’s major urban agglomerations is still very unbalanced and needs more in-depth research. This study used intensive evaluation technology for industrial land in Beijing, Tianjin, and Hebei to evaluate Tianjin and Hebei development zones, and took the Dingzhou Economic Development Zone as an example to evaluate and analyze the intensive use of enterprise land; it suggests the influencing factors for the intensive use of development zones and the measures that can take advantage of the potential of enterprise stock land so as to provide a basis for the rationale behind upgrading industrial land structures and the efficient use of industrial land in Beijing, Tianjin, and Hebei. The results show that the typical enterprise land use in the typical economic development zone is extensive, which suggests that the intensive use of land in the Dingzhou economic development zone is low and that there is still potential for land redevelopment. According to the classification system developed for available land, there is ample opportunity for land-use redevelopment in the development zone, especially in the area of approved but not supplied land and extended enterprise land. Therefore, the redevelopment of enterprise land could also provide a better land guarantee for industrial transfer projects in the equipment manufacturing industry. This study also offers strategies to redevelop available industrial land.
As We Drink and Breathe: Adverse Health Effects of Microcystins and Other Harmful Algal Bloom Toxins in the Liver, Gut, Lungs and Beyond
Authors: Lad Apurva , Breidenbach Joshua D. , Su Robin C. , Murray Jordan , Kuang Rebecca , Mascarenhas Alison , Najjar John , Patel Shivani , Hegde Prajwal , Youssef Mirella , Breuler Jason , Kleinhenz Andrew L. , Ault Andrew P. , Westrick Judy A. , Modyanov Nikolai N. , Kennedy David J. , Haller Steven T.
Published: March 2022

Freshwater harmful algal blooms (HABs) are increasing in number and severity worldwide. These HABs are chiefly composed of one or more species of cyanobacteria, also known as blue-green algae, such as Microcystis and Anabaena. Numerous HAB cyanobacterial species produce toxins (e.g., microcystin and anatoxin—collectively referred to as HAB toxins) that disrupt ecosystems, impact water and air quality, and deter recreation because they are harmful to both human and animal health. Exposure to these toxins can occur through ingestion, inhalation, or skin contact. Acute health effects of HAB toxins have been well documented and include symptoms such as nausea, vomiting, abdominal pain and diarrhea, headache, fever, and skin rashes. While these adverse effects typically increase with amount, duration, and frequency of exposure, susceptibility to HAB toxins may also be increased by the presence of comorbidities. The emerging science on potential long-term or chronic effects of HAB toxins with a particular emphasis on microcystins, especially in vulnerable populations such as those with pre-existing liver or gastrointestinal disease, is summarized herein. This review suggests additional research is needed to define at-risk populations who may be helped by preventative measures. Furthermore, studies are required to develop a mechanistic understanding of chronic, low-dose exposure to HAB toxins so that appropriate preventative, diagnostic, and therapeutic strategies can be created in a targeted fashion.
Can Biological Drugs Diminish the Risk of Sarcopenia in Psoriatic Patients? A Systematic Review
Authors: Piętowska Zuzanna , Nowicka Danuta , Szepietowski Jacek
Published: March 2022

Sarcopenia and psoriasis are different inflammatory diseases that share common comorbidities (e.g., cardiovascular diseases, metabolic syndrome, obesity, autoimmune diseases, depression). Psoriasis is a dermatosis involving the skin, joints, and nails. Its estimated prevalence is 2–4%, and the possibility of progression to psoriatic arthritis reaches 6–42%. Sarcopenia is defined as reduced muscle strength, muscle quantity, and physical performance due to non-ageing related causes. It affects up to 10% of the general population. We conducted a review of the literature to provide up-to-date information about the risk of sarcopenia in psoriasis and to identify risk factors that increase this risk. The search of the literature allowed us to include 51 publications, but only five cross-sectional studies provided quantitative results on the rates of sarcopenia in psoriasis. The prevalence of sarcopenia in psoriasis varied from 9.1% to 61.7%. This wide range was caused by different definitions of sarcopenia and different cut-off values across studies. Prognostic factors include lean mass and fat mass. Further research based on the European Working Group on Sarcopenia in Older People guidelines is required. Such studies should include not only muscle mass and strength but also other factors that may influence the occurrence of sarcopenia and inflammatory markers.
Effect of Dietary Polyphenols on Osteoarthritis—Molecular Mechanisms
Authors: Sirše Mateja
Published: March 2022

Osteoarthritis is a common crippling and degenerative disease resulting in irreversible functional changes due to damage of the cartilage and other tissues of the joint. With limited safe and effective pharmaceutical treatments, the demand and use for alternative therapeutic approaches with symptomatic relief for OA patients have increased. Clinical, pre-clinical, and in vitro studies have demonstrated that polyphenols can exert pain-relieving symptoms coupled with increased functional capacity in OA models. This review will highlight studies carried out in the last five years to define the efficacies and underlying mechanisms in polyphenols such as quercetin, resveratrol, curcumin, epigallocatechin-3-gallate, rosmarinic acid, genistein, ginger, berries, silver fir, pine bark, and Boswellia. Most of these studies indicate that polyphenols exhibit their beneficial roles through regulating changes at the biochemical and molecular levels, inducing or inhibiting various signaling pathways related to inflammation and oxidative stress. Polyphenols have also been implicated in modulating microRNA at the posttranscriptional level to counteract OA pathogenesis.
High-Performance Ni-SiC Coatings Fabricated by Flash Heating
Authors: Renner Peter , Raut Ajinkya , Liang Hong
Published: March 2022

In this research, a novel flash heating coating application technique was utilized to create Ni-SiC coatings on carbon steel substrates with SiC contents much higher than is achievable using certain conventional coating techniques. Hardness profiles showed that the coatings improved the substrate by as much as 121%, without affecting the substrate. Tribotests showed that the wear performance was improved by as much as 4.7× in terms of the wear rate (mm3/N·m) for the same coating when using an Al2O3 counterpart. Pure SiC coatings as a reference were also fabricated. However, the SiC coatings experienced elemental diffusion of Fe from the carbon steel substrate into the coating during fabrication. This occurred due to the increased heat input required for pure SiC to fuse to the substrate compared to the Ni-SiC coatings and resulted in decreased tribological performance. Diffusion of Fe into the coating weakened the coating’s hardness and reduced the resistance to wear. It was concluded that ceramic–metallic composite coatings can successfully be fabricated utilizing this novel flash heating technique to improve the wear resistance of ceramic counterparts.
Long-Term Clearance and Biodistribution of Magnetic Nanoparticles Assessed by AC Biosusceptometry
Authors: Soares Guilherme A. , Faria João V. C. , Pinto Leonardo A. , Prospero Andre G. , Pereira Gabriele M. , Stoppa Erick G. , Buranello Lais P. , Bakuzis Andris F. , Baffa Oswaldo , Miranda José R. A.
Published: March 2022

Once administered in an organism, the physiological parameters of magnetic nanoparticles (MNPs) must be addressed, as well as their possible interactions and retention and elimination profiles. Alternating current biosusceptometry (ACB) is a biomagnetic detection system used to detect and quantify MNPs. The aims of this study were to evaluate the biodistribution and clearance of MNPs profiles through long-time in vivo analysis and determine the elimination time carried out by the association between the ACB system and MnFe2O4 nanoparticles. The liver, lung, spleen, kidneys, and heart and a blood sample were collected for biodistribution analysis and, for elimination analysis, and over 60 days. During the period analyzed, the animal’s feces were also collectedd. It was possible to notice a higher uptake by the liver and the spleen due to their characteristics of retention and uptake. In 60 days, we observed an absence of MNPs in the spleen and a significant decay in the liver. We also determined the MNPs’ half-life through the liver and the spleen elimination. The data indicated a concentration decay profile over the 60 days, which suggests that, in addition to elimination via feces, there is an endogenous mechanism of metabolization or possible agglomeration of MNPs, resulting in loss of ACB signal intensity.
Characterization of Fractal Structures by Spray Flame Synthesis Using X-ray Scattering
Authors: Simmler Mira , Meier Manuel , Nirschl Hermann
Published: March 2022

In this work, we take on an in-depth characterization of the complex particle structures made by spray flame synthesis. Because of the resulting hierarchical aggregates, very few measurement techniques are available to analyze their primary particle and fractal properties. Therefore, we use small-angle X-ray scattering (SAXS) and transmission electron microscopy (TEM) to investigate the influence of the precursor concentration on the fractal structures of zirconia nanoparticles. The combination of information gained from these measurement results leads to a detailed description of the particle system, including the polydispersity and size distribution of the primary particles. Based on our findings, unstable process conditions could be identified at low precursor concentrations resulting in the broadest size distribution of primary particles with rough surfaces. Higher precursor concentrations lead to reproducible primary particle sizes almost independent of the initial precursor concentration. Regarding the fractal properties, the typical shape of aggregates for aerosols is present for the investigated range of precursor concentrations. In conclusion, the consistent results for SAXS and TEM show a conclusive characterization of a complex particle system, allowing for the identification of the underlying particle formation mechanism.
Orodispersible Films with Rupatadine Fumarate Enclosed in Ethylcellulose Microparticles as Drug Delivery Platform with Taste-Masking Effect
Authors: Olechno Katarzyna , Maciejewski Bartosz , Głowacz Klaudia , Lenik Joanna , Ciosek-Skibińska Patrycja , Basa Anna , Winnicka Katarzyna
Published: March 2022

Orally disintegrating (orodispersible) films provide a versatile tool for drug administration, especially in the pediatric and geriatric population, since they reduce the risk of choking and do not necessitate drinking water during application. By considering their direct contact with the taste buds, palatability is an influential aspect related to patient compliance. The microparticles based on taste-masking polymers containing drugs enclosed inside effectively mask the unpleasant taste of medicines. Ethylcellulose is a hydrophobic polymer widely used as a taste-masking material. Rupatadine fumarate, a second-generation antihistamine drug, is characterised by an intense bitter taste; therefore, it is crucial to achieve a tolerable taste whilst developing orodispersible formulations with its content. The objective of this study was to develop orally disintegrating films with rupatadine fumarate in the form of ethylcellulose-based microparticles obtained from aqueous dispersions of ethylcellulose—Surelease® or Aquacoat® ECD. It was a technological challenge to achieve homogenous drug content per dosage unit and sufficient mechanical properties for film operating due to the necessity to suspend the microparticles in the casting solution. Although the process of obtaining films consisted of several steps (mixing, pouring, drying), the particles were homogeneously dispersed, and each film of the desired size contained the proper dose of the drug. The taste-masking effect was also maintained. This parameter was confirmed by three independent methods: in vivo by healthy volunteers, an electronic tongue and a dissolution test. The applied taste-evaluation techniques showed that the films containing Aquacoat® ECD microparticles have the highest degree of bitter taste reduction, which confirms the results obtained in our previous studies.
A Review of Ultrafine-Grained Magnetic Materials Prepared by Using High-Pressure Torsion Method
Authors: Wang Zhi-Rui , Si Ping-Zhan , Park Jihoon , Choi Chul-Jin , Ge Hong-Liang
Published: March 2022

High-pressure torsion (HPT) is a severe plastic deformation technique where a sample is subjected to torsional shear straining under a high hydrostatic pressure. The HPT method is usually employed to create ultrafine-grained nano-structures, making it widely used in processing many kinds of materials such as metals, glasses, biological materials, and organic compounds. Most of the published HPT results have been focused on the microstructural development of non-magnetic materials and their influence on the mechanical properties. The HPT processing of magnetic materials and its influence on the structural and magnetic properties have attracted increasing research interest recently. This review describes the application of HPT to magnetic materials and our recent experimental results on Mn3O4, Mn4N, and MnAl-based alloys. After HPT, most magnetic materials exhibit significantly reduced grain size and substantially enhanced coercivity.
Biomedical Ti-Nb-Zr Foams Prepared by Means of Thermal Dealloying Process and Electrochemical Modification
Authors: Adamek Grzegorz , Junka Adam , Wirstlein Przemyslaw , Jurczyk Mieczyslawa U. , Siwak Piotr , Koper Jeremiasz , Jakubowicz Jaroslaw
Published: March 2022

The paper presents results of preparation and modification of Ti20Nb5Zr foams by a thermal dealloying method followed by electrochemical modification. The first step of this study was the preparation of Ti20Nb5Zr30Mg nanopowder using mechanical alloying (MA). The second was forming green compacts by cold pressing and then sintering with magnesium dealloyed from the structure, which resulted in pores formation. The next step was surface modification by electrochemical etching and silver nanoparticle deposition. Porosity, morphology, mechanical properties as well as biocompatibility and antibacterial behavior were investigated. Titanium foam porosity up to approximately 60% and wide pore size distribution were successfully prepared. The new materials have shown positive behavior in the MTT assay as well as antibacterial properties. These results confirmed great potential for thermal dealloying in preparation of porous structures.
Resistance to High-Temperature Oxidation of Ti-Al-Nb Alloys
Authors: Małecka Joanna
Published: March 2022

The research presented in this paper concerns the assessment of the resistance to high-temperature oxidation behaviour of a Ti-46Al-7Nb-0.7Cr-0.1Si-0.2Ni alloy and the explanation of the role of niobium during oxidation processes. The basic problem concerned the evaluation of the resistance of the studied alloy to cyclic oxidation in an air atmosphere, with particular attention to the influence of temperature, surface roughness and cooling rate from heating temperature to room temperature. The issue analysed was the effect of niobium addition on the corrosion kinetics as a high-melting element causing improved oxidation resistance, contributing to the reduction in the oxidation rate.
Biofilm on Toothbrushes of Children with Cystic Fibrosis: A Potential Source of Lung Re-Infection after Antibiotic Treatment?
Authors: Hu Honghua , Clothier Nicole , Jacombs Anita , Mckay Karen , Deva Anand K. , Vickery Karen
Published: March 2022

Frequent recurrent lung infections result in irreversible lung damage in children with cystic fibrosis (CF). This study aimed to determine if toothbrushes contain biofilms of pathogens, and act as potential reservoirs for lung re-infection following antibiotic treatment of acute exacerbations. Toothbrushes were collected from children with CF of lung infection before, during and after antibiotic treatment. Toothbrushes were rinsed with sterile saline and cultured. Bacterial isolates from toothbrushes were identified by 16s rRNA gene sequencing and compared with isolates from a sputum sample of the same patient. Scanning electron microscopy (SEM) was used to visually confirm the presence of bacterial biofilms and confocal laser scanning microscopy (CLSM) combined with Live/Dead stain to confirm bacterial viability. Large numbers of bacteria and biofilms were present on all toothbrushes. SEM confirmed the presence of biofilms and CLSM confirmed bacterial viability on all toothbrushes. Pathogens identified on toothbrushes from children before and during antibiotics treatment were in concordance with the species found in sputum samples. Pseudomonas aeruginosa and Staphylococcus aureus was able to be cultured from children’s toothbrushes despite antibiotic treatment. Toothbrushes were shown to be contaminated with viable pathogens and biofilms before and during antibiotic treatment and could be a potential source of lung re-infections.
Efficient Use of Graphene Oxide in Layered Cement Mortar
Authors: Liu Shuangshuang , Lu Fenglei , Chen Ya , Dong Biqin , Du Hongxiu , Li Xiangyu
Published: March 2022

Graphene oxide (GO) has been found to be an attractive nanomaterial to improve the properties of cementitious composites. However, the use of GO in the industry is limited by its high cost. To achieve a higher cost/performance ratio, GO can be strategically applied in certain parts of cementitious composites structure according to the principle of functionally graded materials. In this study, graded distribution of GO in cement mortar was achieved by sequentially casting a fresh GO-incorporated cement layer on another cement mortar layer. The mechanical properties, especially flexural strength, of layered cement mortar were found to be dependent on the GO content, the delay time, and the interface formed due to layering fabrication. With the GO incorporated in the tensile region only (30% of the total depth), the flexural strength of the layered beam attained 90.91% of that of the beam, with GO uniformly distributed throughout the sample. Based on the results of rapid chloride migration tests, when 12 mm GO-incorporated cement mortar layer was used, the chloride migration coefficient was reduced by 21.45%. It was also found that the measured chloride migration coefficient of layered cement mortar agreed with the series model. The present investigation provides an efficient approach to use GO in cement-based materials from the perspective of mechanical and durability properties.