bluuebunny/crossref_metadata_2025 huggingface dataset:
The Janus Face of IL-33 Signaling in Tumor Development and Immune Escape
Authors: Choi Mi-Ran , Sosman Jeffrey A. , Zhang Bin
Published: June 2021

Interleukin-33 (IL-33), a member of the IL-1 cytokine family, plays a critical role in maintaining tissue homeostasis as well as pathological conditions, such as allergy, infectious disease, and cancer, by promoting type 1 and 2 immune responses. Through its specific receptor ST2, IL-33 exerts multifaceted functions through the activation of diverse intracellular signaling pathways. ST2 is expressed in different types of immune cells, including Th2 cells, Th1 cells, CD8+ T cells, regulatory T cells (Treg), cytotoxic NK cells, group 2 innate lymphoid cells (ILC2s), and myeloid cells. During cancer initiation and progression, the aberrant regulation of the IL-33/ST2 axis in the tumor microenvironment (TME) extrinsically and intrinsically mediates immune editing via modulation of both innate and adaptive immune cell components. The summarized results in this review suggest that IL-33 exerts dual-functioning, pro- as well as anti-tumorigenic effects depending on the tumor type, expression levels, cellular context, and cytokine milieu. A better understanding of the distinct roles of IL-33 in epithelial, stromal, and immune cell compartments will benefit the development of a targeting strategy for this IL-33/ST2 axis for cancer immunotherapy.
Natural Merosesquiterpenes Activate the DNA Damage Response via DNA Strand Break Formation and Trigger Apoptotic Cell Death in p53-Wild-Type and Mutant Colorectal Cancer
Authors: Jiso Apisada , Demuth Philipp , Bachowsky Madeleine , Haas Manuel , Seiwert Nina , Heylmann Daniel , Rasenberger Birgit , Christmann Markus , Dietrich Lea , Brunner Thomas , Riyanti  , Schäberle Till F. , Plubrukarn Anuchit , Fahrer Jörg
Published: June 2021

Colorectal cancer (CRC) is a frequently occurring malignant disease with still low survival rates, highlighting the need for novel therapeutics. Merosesquiterpenes are secondary metabolites from marine sponges, which might be useful as antitumor agents. To address this issue, we made use of a compound library comprising 11 isolated merosesquiterpenes. The most cytotoxic compounds were smenospongine > ilimaquinone ≈ dactylospontriol, as shown in different human CRC cell lines. Alkaline Comet assays and γH2AX immunofluorescence microscopy demonstrated DNA strand break formation in CRC cells. Western blot analysis revealed an activation of the DNA damage response with CHK1 phosphorylation, stabilization of p53 and p21, which occurred both in CRC cells with p53 knockout and in p53-mutated CRC cells. This resulted in cell cycle arrest followed by a strong increase in the subG1 population, indicative of apoptosis, and typical morphological alterations. In consistency, cell death measurements showed apoptosis following exposure to merosesquiterpenes. Gene expression studies and analysis of caspase cleavage revealed mitochondrial apoptosis via BAX, BIM, and caspase-9 as the main cell death pathway. Interestingly, the compounds were equally effective in p53-wild-type and p53-mutant CRC cells. Finally, the cytotoxic activity of the merosesquiterpenes was corroborated in intestinal tumor organoids, emphasizing their potential for CRC chemotherapy.
Copy Number Alteration Profile Provides Additional Prognostic Value for Acute Lymphoblastic Leukemia Patients Treated on BFM Protocols
Authors: Αmpatzidou Μirella , Florentin Lina , Papadakis Vassilios , Paterakis Georgios , Tzanoudaki Marianna , Bouzarelou Dimitra , Papadhimitriou Stefanos I. , Polychronopoulou Sophia
Published: June 2021

We present our data of a novel proposed CNA-profile risk-index, applied on a Greek ALLIC-BFM-treated cohort, aiming at further refining genomic risk-stratification. Eighty-five of 227 consecutively treated ALL patients were analyzed for the copy-number-status of eight genes (IKZF1/CDKN2A/2B/PAR1/BTG1/EBF1/PAX5/ETV6/RB1). Using the MLPA-assay, patients were stratified as: (1) Good-risk(GR)-CNA-profile (n = 51), with no deletion of IKZF1/CDKN2A/B/PAR1/BTG1/EBF1/PAX5/ETV6/RB1 or isolated deletions of ETV6/PAX5/BTG1 or ETV6 deletions with a single additional deletion of BTG1/PAX5/CDKN2A/B. (2) Poor-risk(PR)-CNA-profile (n = 34), with any deletion of ΙΚΖF1/PAR1/EBF1/RB1 or any other CNA. With a median follow-up time of 49.9 months, EFS for GR-CNA-profile and PR-CNA-profile patients was 96.0% vs. 57.6% (p < 0.001). For IR-group and HR-group patients, EFS for the GR-CNA/PR-CNA subgroups was 100.0% vs. 60.0% (p < 0.001) and 88.2% vs. 55.6% (p = 0.047), respectively. Among FC-MRDd15 + patients (MRDd15 ≥ 10−4), EFS rates were 95.3% vs. 51.7% for GR-CNA/PR-CNA subjects (p < 0.001). Similarly, among FC-MRDd33 + patients (MRDd33 ≥ 10−4), EFS was 92.9% vs. 27.3% (p < 0.001) and for patients FC-MRDd33 − (MRDd33 < 10−4), EFS was 97.2% vs. 72.7% (p = 0.004), for GR-CNA/PR-CNA patients, respectively. In a multivariate analysis, the CNA-profile was the most important outcome predictor. In conclusion, the CNA-profile can establish a new genomic risk-index, identifying a distinct subgroup with increased relapse risk among the IR-group, as well as a subgroup of patients with superior prognosis among HR-patients. The CNA-profile is feasible in BFM-based protocols, further refining MRD-based risk-stratification.
Ancillary Diagnostic Investigations in Malignant Pleural Mesothelioma
Authors: Dipper Alex , Maskell Nick , Bibby Anna
Published: June 2021

For a number of patients presenting with an undiagnosed pleural effusion, frailty, medical co-morbidity or personal choice may preclude the use of pleural biopsy, the gold standard investigation for diagnosis of malignant pleural mesothelioma (MPM). In this review article, we outline the most recent evidence on ancillary diagnostic tests which may be used to support a diagnosis of MPM where histological samples cannot be obtained or where results are non-diagnostic. Immunocytochemical markers, molecular techniques, diagnostic biomarkers and imaging techniques are discussed. No adjunctive test has a sensitivity and specificity profile to support use in isolation; however, correlation of pleural fluid cytology with relevant radiology and supplementary biomarkers can enable an MDT-consensus clinico-radiological-cytological diagnosis to be made where further invasive tests are not possible or not appropriate. Diagnostic challenges surrounding non-epithelioid MPM are recognised, and there is a critical need for reliable and non-invasive investigative tools in this population.
Metastatic Risk Factors Associated with Class 1A Uveal Melanoma Patients
Authors: Ballhausen Alexej , Urias Elizabeth , Gruschkus Stephen K. , Williams Michelle , Glover Maura S. , Qin Yong , Gombos Dan S. , Patel Sapna P.
Published: June 2021

In uveal melanoma (UM), gene expression profiling (GEP) is commonly used to classify metastatic risk into three groups (Class 1A, 1B, and 2). Class 1A patients have a lower metastatic risk of 2% at 5 years compared to other groups. We aimed to describe clinical features associated with the development of metastasis in this low-risk group. This single-center IRB-approved retrospective case series review included all UM patients between February 2006 and March 2019 with an archived or fresh specimen classified as Class 1A. Cox regression and receiver operating characteristics analyses were used to identify factors associated with metastasis development and OS. Among 73 UM patients with Class 1A, the 5-year cumulative incidence of local recurrence and distant metastasis was 4.2% and 17.0%, respectively. Stage III disease (HR 20.7; 95% confidence interval (95% CI) 1.4–300.6; p= 0.0264) was found to be independently associated with metastatic recurrence, while primary therapy was associated with OS (enucleation vs. brachytherapy, HR 13.5; 95% CI 1.3–147.6; p = 0.0348). Combined clinical decision-making utilizing factors such as GEP class, American Joint Committee on Cancer (AJCC) stage, and COMS size could have a significant clinical impact by improving risk stratification and adapting follow-up intervals in UM Class 1A patients.
Feasibility of Temperature Control by Electrical Impedance Tomography in Hyperthermia
Authors: Poni Redi , Neufeld Esra , Capstick Myles , Bodis Stephan , Samaras Theodoros , Kuster Niels
Published: June 2021

We present a simulation study investigating the feasibility of electrical impedance tomography (EIT) as a low cost, noninvasive technique for hyperthermia (HT) treatment monitoring and adaptation. Temperature rise in tissues leads to perfusion and tissue conductivity changes that can be reconstructed in 3D by EIT to noninvasively map temperature and perfusion. In this study, we developed reconstruction methods and investigated the achievable accuracy of EIT by simulating HT treatmentlike scenarios, using detailed anatomical models with heterogeneous conductivity distributions. The impact of the size and location of the heated region, the voltage measurement signal-to-noise ratio, and the reference model personalization and accuracy were studied. Results showed that by introducing an iterative reconstruction approach, combined with adaptive prior regions and tissue-dependent penalties, planning-based reference models, measurement-based reweighting, and physics-based constraints, it is possible to map conductivity-changes throughout the heated domain, with an accuracy of around 5% and cm-scale spatial resolution. An initial exploration of the use of multifrequency EIT to separate temperature and perfusion effects yielded promising results, indicating that temperature reconstruction accuracy can be in the order of 1 °C. Our results suggest that EIT can provide valuable real-time HT monitoring capabilities. Experimental confirmation in real-world conditions is the next step.
Role of a Heat Shock Transcription Factor and the Major Heat Shock Protein Hsp70 in Memory Formation and Neuroprotection
Authors: Zatsepina Olga G. , Evgen’ev Michael B. , Garbuz David G.
Published: June 2021

Heat shock proteins (Hsps) represent the most evolutionarily ancient, conserved, and universal system for protecting cells and the whole body from various types of stress. Among Hsps, the group of proteins with a molecular weight of 70 kDa (Hsp70) plays a particularly important role. These proteins are molecular chaperones that restore the native conformation of partially denatured proteins after exposure to proteotoxic forms of stress and are critical for the folding and intracellular trafficking of de novo synthesized proteins under normal conditions. Hsp70s are expressed at high levels in the central nervous system (CNS) of various animals and protect neurons from various types of stress, including heat shock, hypoxia, and toxins. Numerous molecular and behavioral studies have indicated that Hsp70s expressed in the CNS are important for memory formation. These proteins contribute to the folding and transport of synaptic proteins, modulate signaling cascades associated with synaptic activation, and participate in mechanisms of neurotransmitter release. In addition, HSF1, a transcription factor that is activated under stress conditions and mediates Hsps transcription, is also involved in the transcription of genes encoding many synaptic proteins, whose levels are increased in neurons under stress and during memory formation. Thus, stress activates the molecular mechanisms of memory formation, thereby allowing animals to better remember and later avoid potentially dangerous stimuli. Finally, Hsp70 has significant protective potential in neurodegenerative diseases. Increasing the level of endogenous Hsp70 synthesis or injecting exogenous Hsp70 reduces neurodegeneration, stimulates neurogenesis, and restores memory in animal models of ischemia and Alzheimer’s disease. These findings allow us to consider recombinant Hsp70 and/or Hsp70 pharmacological inducers as potential drugs for use in the treatment of ischemic injury and neurodegenerative disorders.
Fatal COVID-19 in a Child with Persistence of SARS-CoV-2 Despite Extensive Multidisciplinary Treatment: A Case Report
Authors: Apostolidou Sofia , Harbauer Theresa , Lasch Peter , Biermann Daniel , Hempel Maja , Lütgehetmann Marc , Pfefferle Susanne , Herrmann Jochen , Rüffer André , Reinshagen Konrad , Kozlik-Feldmann Rainer , Gieras Anna , Kniep Inga , Oh Jun , Singer Dominique , Ebenebe Chinedu Ulrich , Kobbe Robin
Published: June 2021

Critical Coronavirus disease 2019 (COVID-19) developed in a 7-year-old girl with a history of dystrophy, microcephaly, and central hypothyroidism. Starting with gastrointestinal symptoms, the patient developed severe myocarditis followed by progressive multiple organ failure complicated by Pseudomonas aeruginosa bloodstream infection. Intensive care treatment consisting of invasive ventilation, drainage of pleural effusion, and high catecholamine therapy could not prevent the progression of heart failure, leading to the implantation of venoarterial extracorporeal life support (VA-ECLS) and additional left ventricle support catheter (Impella® pump). Continuous venovenous hemofiltration (CVVH) and extracorporeal hemadsorption therapy (CytoSorb®) were initiated. Whole exome sequencing revealed a mutation of unknown significance in DExH-BOX helicase 30 (DHX30), a gene encoding a RNA helicase. COVID-19 specific antiviral and immunomodulatory treatment did not lead to viral clearance or control of hyperinflammation resulting in the patient’s death on extracorporeal life support-(ECLS)-day 20. This fatal case illustrates the potential severity of pediatric COVID-19 and suggests further evaluation of antiviral treatment strategies and vaccination programs for children.
Preparation of High-Transparency, Superhydrophilic Visible Photo-Induced Photocatalytic Film via a Rapid Plasma-Modification Process
Authors: Lu Bing-Jyh , Lin Keng-Ta , Kuo Yi-Ming , Tsai Cheng-Hsien
Published: June 2021

In this study, different amounts of SiO2 nanoparticles (7 nm) were added to simultaneously reach high transmittance, high hardness, and high adhesion for TiO2 film prepared by the sol–gel method and coated on glass through a dip-coating technique. For the film to achieve self-cleaning, anti-fogging, superhydrophilicity, and visible photo-induced photocatalysis, TiO2-SiO2 film was modified via a rapid microwave plasma-nitridation process for efficient N-doping by various N2-containing gases (N2, N2/Ar/O2, N2/Ar). Through nitrogen plasma, the content of N atom reached 1.3% with the ratio of O/Ti atom being 2.04. The surface of the thin films was smooth, homogeneous, and did not crack, demonstrated by the root mean square (RMS) roughness of film surface being 3.29–3.94 nm. In addition, the films were composed of nanoparticles smaller than 10 nm, with a thickness of about 100 nm, as well as the crystal phase of the thin film being anatase. After the plasma-nitridation process, the visible-light transmittance of N-doped TiO2-SiO2 films was 89.7% (clean glass = 90.1%). Moreover, the anti-fogging ability was excellent (contact angle < 5°) even without light irradiation. The degradation of methylene blue showed that the photocatalytic performance of N-doped TiO2-SiO2 films was apparently superior to that of unmodified films under visible-light irradiation. Moreover, the pencil hardness and adhesion rating test of the thin films were 7H and 5B, respectively, indicating that the obtained coatings had great mechanical stability.
Antiferromagnetic Oxide Thin Films for Spintronic Applications
Authors: Siddiqui Saima Afroz , Hong Deshun , Pearson John E. , Hoffmann Axel
Published: June 2021

Antiferromagnetic oxides have recently gained much attention because of the possibility to manipulate electrically and optically the Néel vectors in these materials. Their ultrafast spin dynamics, long spin diffusion length and immunity to large magnetic fields make them attractive candidates for spintronic applications. Additionally, there have been many studies on spin wave and magnon transport in single crystals of these oxides. However, the successful applications of the antiferromagnetic oxides will require similar spin transport properties in thin films. In this work, we systematically show the sputtering deposition method for two uniaxial antiferromagnetic oxides, namely Cr2O3 and α-Fe2O3, on A-plane sapphire substrates, and identify the optimized deposition conditions for epitaxial films with low surface roughness. We also confirm the antiferromagnetic properties of the thin films. The deposition method developed in this article will be important for studying the magnon transport in these epitaxial antiferromagnetic thin films.
The Enrichment of (Cu, Sn) Solid Solution Driven by High-Pressure Torsion
Authors: Straumal Boris , Kilmametov Askar , Korneva Anna , Zięba Pawel , Zavorotnev Yuri , Metlov Leonid , Popova Olga , Baretzky Brigitte
Published: June 2021

Cu–14 wt% Sn alloy was annealed at temperatures of 320 and 500 °C. The concentration of tin cinit in the copper-based matrix increased with annealing temperature. The annealed samples were subjected to high-pressure torsion (HPT) at 6 GPa, 5 turns, 1 rpa. HPT led to the refinement of Cu grains. The shape of the colonies of α + ε phases changed only slightly. The HPT-driven enrichment of the Cu-based solid solution with Sn atoms cHPT–cinit decreased with increasing cinit. The performed theoretical analysis explained this behavior of the HPT-driven enrichment.
Neuroanatomical Study and Three-Dimensional Reconstruction of the Skull of a Bothremydid Turtle (Pleurodira) Based on the European Eocene Tartaruscola teodorii
Authors: Martín-Jiménez Marcos , Pérez-García Adán
Published: June 2021

Bothremydidae is a successful lineage of pleurodiran turtles that lived from the Cretaceous to the Paleogene, and are found in most continents of both Laurasia and Gondwana. Despite numerous known cranial remains of this clade, no study on the complete neuroanatomical reconstruction of any of its representatives has been published so far. Tartaruscola teodorii is a French lower Eocene member of Foxemydina (Bothremydini), known by two skulls. It is one of the few bothremydids identified in the Cenozoic record of Europe. The present study includes the complete three-dimensional reconstruction of each of the cranial bones of both the holotype and the paratype of T. teodorii, increasing the anatomical information about this species. The virtual reconstruction of its neuroanatomical structures is presented here, including the cranial cavity, nerves, nasal cavity, inner ears, and carotid arteries. This analysis is the first detailed neuroanatomical study performed for a member of Bothremydidae. In addition, the virtual reconstruction of the neuroanatomical structures of some extant taxa belonging to several pleurodiran lineages (Chelidae, Pelomedusidae and Podocnemididae), are also carried out and analyzed, so that the comparative framework for Pleurodira is remarkably improved.
Variation in Dairy Milk Composition and Properties Has Little Impact on Cheese Ripening: Insights from a Traditional Swedish Long-Ripening Cheese
Authors: Priyashantha Hasitha , Johansson Monika , Langton Maud , Sampels Sabine , Jayarathna Shishanthi , Hetta Mårten , Saedén Karin Hallin , Höjer Annika , Lundh Åse
Published: June 2021

The monthly variation in raw dairy silo milk was investigated and related to the ripening time of the resulting cheese during an industrial cheese-making trial. Milk composition varied with month, fat and protein content being lowest in August (4.19 and 3.44 g/100 g, respectively). Casein micelle size was largest (192–200 nm) in December–February and smallest (80 nm) in August. In addition, SCC, total bacteria count, proteolytic activities, gel strength, and milk fatty acid composition were significantly varied with month. Overall sensory and texture scores of resulting cheese were mainly influenced by plasmin and plasminogen activity, indicating the importance of native proteolytic systems. Recently, concepts based on the differentiated use of milk in dairy products have been suggested. For the investigated cheese type, there might be little to gain from such an approach. The variation in the investigated quality characteristics of the dairy milk used for cheese making had little effect on cheese ripening in our study. In contrast to our hypothesis, we conclude that as long as the quality of the milk meets certain minimum criteria, there are only weak associations between cheese milk characteristics and the time required for the development of aroma and texture in the cheese. To find answers behind the observed variation in cheese ripening time, studies on the effects of process parameters are needed.
The Combination of SOFA Score and Urinary NGAL May Be an Effective Predictor for Ventilator Dependence among Critically Ill Surgical Patients: A Pilot Study
Authors: Tsai Hsin-I , Lu Yu-Chieh , Kou Hao-Wei , Hsu Heng-Yuan , Huang Song-Fong , Huang Chun-Wei , Lee Chao-Wei
Published: June 2021

Background: Ventilator dependence (VD) has been considered as a serious complication in critically ill patients in the intensive care unit (ICU). Acute kidney injury (AKI) is associated with VD as a result of lung–kidney interaction. The aim of our study was to investigate novel biomarkers in predicting ventilator dependence in critically ill surgical patients. Methods: Patients who were admitted to surgical ICU were enrolled and their serum and urine samples were collected. Novel biomarkers including gelatinase-associated lipocalin (NGAL), calprotectin, kidney injury molecule-1 (KIM-1), cystatin C, and growth differentiation factor 15 (GDF-15) were analyzed and correlated with clinical outcome. Results: A total of 33 patients were enrolled and analyzed. The majority of them received abdominal surgery prior to ICU admission. Thirteen patients were classified into the VD group, while the remaining 20 were in a non-ventilator dependence group (nVD). Statistical analysis demonstrated that the following were significantly higher in the VD group than in the nVD group: serum NGAL (420.25 ± 45.18 ng/mL vs. 314.68 ± 38.12 ng/mL, p-value 0.036), urinary NGAL (420.87 ± 41.08 ng/mL vs. 250.84 ± 39.45 ng/mL, p-value 0.002), SOFA score (11.3 ± 1.5 vs. 5.6 ± 0.7, p-value 0.001), and APACHE II score (23.2 ± 2.6 vs. 13.6 ± 0.8, p-value 0.001). The area under the ROC curve (AUROC) of urinary NGAL for VD was 0.808. The combination of urinary NGAL and SOFA score could further increase AUROC for VD to 0.835. Conclusions: The current study demonstrated the predictive capability of urinary NGAL for ventilator dependence among critically ill surgical patients. When combined with SOFA score, the predictive ability was further augmented. Further large-scale studies are warranted to validate our findings.
ACO (Asthma–COPD Overlap) Is Independent from COPD: The Case Against
Authors: Calverley Peter M. A. , Walker Paul Phillip
Published: June 2021

Over the last decade interest has been shown in people with symptomatic lung disease who have features both of COPD and asthma. In this review we examine how COPD and asthma are defined and examine clinical characteristics of people defined by researchers as having asthma-COPD overlap (ACO). We look at pathological and physiological features along with symptoms and consider the impact of each diagnosis upon therapeutic management. We highlight challenges in the diagnosis and management of airway disease and the various phenotypes that could be part of ACO, in so doing suggesting ways for the clinician to manage patients with features of both asthma and COPD.
Trade–Climate Nexus: A Systematic Review of the Literature
Authors: Balogh Jeremiás Máté , Mizik Tamás
Published: June 2021

In the climate–trade debate, moderate attention is dedicated to the role of trade agreements on climate. In turn, trade agreements could help countries meet climate goals by removing tariffs, harmonizing standards on environmental goods, and eliminating distorting subsidies on fossil fuels. This paper aims to provide an overview of the role of trade agreements on climate-change mitigation. This systematic literature review is based on the international economic literature published between 2010 and 2020. This literature review underlines that the effectiveness of the trade agreements and WTO negotiations on emission reduction is weak. This is due to different national interests and protectionism. The elimination of trade barriers stimulates trade, but this may also raise greenhouse gas emissions and cause other environmental problems (e.g., deforestation). Furthermore, this article points out that emission leakage is also a crucial issue hindering the success of global climate agreements on greenhouse gas reduction. The greatest beneficiaries of the trade agreements are usually the largest GHG emitters, such as China, the US, and the EU. By contrast, developing countries are in a weaker position regarding climate–trade negotiation. The literature review offers policy solutions which can contribute to emission reduction and tools for stimulating a trade-related climate-change abatement policy.
Educational Practices for Immigrant Children in Elementary Schools in Russia
Authors: Gromova Chulpan , Khairutdinova Rezeda , Birman Dina , Kalimullin Aydar
Published: June 2021

Teachers have a pivotal role in the acculturation and adjustment of immigrant children. Practices are an important but an insufficiently explored part of teachers’ work in a multicultural classroom. The purpose of the present research was to identify educational practices that elementary school teachers in the Republic of Tatarstan, Russia, use in their work with immigrant children to provide language and academic support and promote a welcoming atmosphere in the classroom that fosters psychological adjustment of the child. Data were collected through interviews with twenty elementary school teachers working with immigrant children. Interviews were analyzed using inductive and deductive content analysis methods. Findings suggest that in the absence of institutionalized structures, teachers take the initiative to adapt their teaching and instruction methods when working with immigrant children. Teachers primarily rely on individual (one-on-one) tutoring methods to provide language and academic support. Approaches to creating a favorable climate in the classroom and the child’s psychological adjustment include practices of promoting respect for different ethnic groups and developing cross-cultural communication skills. Inclusion of parents in the educational process is used in conjunction with all practices with immigrant children used by teachers. In addition, teachers often rely on Tatar language as an intermediary between the migrant children’s heritage language and Russian when communicating with them. Most children of immigrants are from Central Asian countries where the languages spoken are Turkic in origin and similar to Tatar—the indigenous language spoken in the Republic of Tatarstan.
Textile Antenna-Sensor for In Vitro Diagnostics of Diabetes
Authors: Gharbi Mariam El , Fernández-García Raúl , Gil Ignacio
Published: June 2021

In this paper, a feasibility study of a microwave antenna-based sensor is proposed for in vitro experiments for monitoring blood glucose levels. The proposed device consists of a square-ring incorporated within a fully textile monopole antenna to absorb and sense different glucose concentrations, covering patients with different diabetic conditions. The designed antenna-sensor is optimized to operate at 2.4 GHz. The sensing principle is based on the resonance frequency shift of the reflection response of the antenna-based sensor under different glucose levels. The experiments were carried out with blood mimicking by means of aqueous solutions, using D(+)- glucose/water in different concentrations for various diabetic conditions of type-2 diabetes. The performance of the embroidered antenna-based sensor is characterized and validated using a convenient setup for in vitro measurements. The results demonstrated the ability of the proposed antenna-based sensor to cover all the glucose levels of the diabetes range, including hypoglycemia (10–70 mg/dL), normoglycemia (80–110 mg/dL) and hyperglycemia (130–190 mg/dL) with a sensitivity of 350 kHz/(mg/dL). Besides its ability to detect different glucose concentrations of various diabetic conditions, the proposed antenna-sensor presents diverse features such as a simplistic design, compact size, wearability and low cost. The proposed textile device demonstrates a proof of concept for efficient in vitro blood glucose level measurements and diagnostics of diabetes.
An Efficient Convolutional Neural Network Model Combined with Attention Mechanism for Inverse Halftoning
Authors: Shao Linhao , Zhang Erhu , Li Mei
Published: June 2021

Inverse halftoning acting as a special image restoration problem is an ill-posed problem. Although it has been studied in the last several decades, the existing solutions can’t restore fine details and texture accurately from halftone images. Recently, the attention mechanism has shown its powerful effects in many fields, such as image processing, pattern recognition and computer vision. However, it has not yet been used in inverse halftoning. To better solve the problem of detail restoration of inverse halftoning, this paper proposes a simple yet effective deep learning model combined with the attention mechanism, which can better guide the network to remove noise dot-patterns and restore image details, and improve the network adaptation ability. The whole model is designed in an end-to-end manner, including feature extraction stage and reconstruction stage. In the feature extraction stage, halftone image features are extracted and halftone noises are removed. The reconstruction stage is employed to restore continuous-tone images by fusing the feature information extracted in the first stage and the output of the residual channel attention block. In this stage, the attention block is firstly introduced to the field of inverse halftoning, which can make the network focus on informative features and further enhance the discriminative ability of the network. In addition, a multi-stage loss function is proposed to accelerate the network optimization, which is conducive to better reconstruction of the global image. To demonstrate the generalization performance of the network for different types of halftone images, the experiment results confirm that the network can restore six different types of halftone image well. Furthermore, experimental results show that our method outperforms the state-of-the-art methods, especially in the restoration of details and textures.
Machine Learning Classification and Regression Approaches for Optical Network Traffic Prediction
Authors: Szostak Daniel , Włodarczyk Adam , Walkowiak Krzysztof
Published: June 2021

Rapid growth of network traffic causes the need for the development of new network technologies. Artificial intelligence provides suitable tools to improve currently used network optimization methods. In this paper, we propose a procedure for network traffic prediction. Based on optical networks’ (and other network technologies) characteristics, we focus on the prediction of fixed bitrate levels called traffic levels. We develop and evaluate two approaches based on different supervised machine learning (ML) methods—classification and regression. We examine four different ML models with various selected features. The tested datasets are based on real traffic patterns provided by the Seattle Internet Exchange Point (SIX). Obtained results are analyzed using a new quality metric, which allows researchers to find the best forecasting algorithm in terms of network resources usage and operational costs. Our research shows that regression provides better results than classification in case of all analyzed datasets. Additionally, the final choice of the most appropriate ML algorithm and model should depend on the network operator expectations.
Inclusion of Shipping in the EU-ETS: Assessing the Direct Costs for the Maritime Sector Using the MRV Data
Authors: Christodoulou Anastasia , Dalaklis Dimitrios , Ölçer Aykut I. , Ghaforian Masodzadeh Peyman
Published: June 2021

This paper aims to assess the direct economic impact on the maritime sector from its inclusion in the European Union-Emission Trading System (EU-ETS). The Monitoring, Reporting and Verification (MRV) data are analysed for the estimation of carbon dioxide (CO2) emissions within the European Economic Area (EEA). The economic impact assessment model used is scenario-based, and includes different price incentives, geographical coverage and emission allowances allocation methods. According to our findings, in case the emission allowances are fully auctioned or partially free allocated on the basis of a uniform benchmark, the increased costs would be disproportional among the maritime segments. Such a scheme would penalise Roll-on/Roll-off (RoRo) and Roll-On/Roll-Off/Passenger (RoPax) segments due their high fuel consumption per transport work in relation to oil tankers and bulkers. The establishment of differentiated benchmarks per segment seems to be a prerequisite for the effective inclusion of shipping in the EU-ETS that will reward energy efficient vessels in each segment and avoid competition distortion within the maritime industry.
Enhanced Boost Factor for Three-Level Quasi-Switched Boost T-Type Inverter
Authors: Do Duc-Tri , Tran Vinh-Thanh , Nguyen Minh-Khai
Published: June 2021

A new modulation strategy has been introduced in this paper in order to enhance the boost factor for the three-level quasi-switched boost T-type inverter (3L-qSBT2I). Under this approach, the component rating of power devices is significantly decreased. Moreover, the use of a larger boost factor produces a smaller shoot-through current. This benefit leads to reducing the conduction loss significantly. Furthermore, the neutral voltage unbalance is also considered. The duty cycle of two active switches of a quasi-switched boost (qSB) network is redetermined based on actual capacitor voltages to recovery balance condition. Noted that the boost factor will not be affected by the proposed capacitor voltage balance strategy. The proposed method is taken into account to be compared with other previous studies. The operation principle and overall control strategy for this configuration are also detailed. The simulation and experiment are implemented with the help of PSIM software and laboratory prototype to demonstrate the accuracy of this strategy.
Comparative Analysis of the Complete Chloroplast Genomes of Four Chestnut Species (Castanea)
Authors: Zhou Huijuan , Gao Xiaoxiao , Woeste Keith , Zhao Peng , Zhang Shuoxin
Published: June 2021

Chloroplast (cp) DNA genomes are traditional workhorses for studying the evolution of species and reconstructing phylogenetic relationships in plants. Species of the genus Castanea (chestnuts and chinquapins) are valued as a source of nuts and timber wherever they grow, and chestnut species hybrids are common. We compared the cp genomes of C. mollissima, C. seguinii, C. henryi, and C. pumila. These cp genomes ranged from 160,805 bp to 161,010 bp in length, comprising a pair of inverted repeat (IR) regions (25,685 to 25,701 bp) separated by a large single-copy (LSC) region (90,440 to 90,560 bp) and a small single-copy (SSC) region (18,970 to 19,049 bp). Each cp genome encoded the same 113 genes; 82–83 protein-coding genes, 30 transfer RNA genes, and four ribosomal RNA genes. There were 18 duplicated genes in the IRs. Comparative analysis of cp genomes revealed that rpl22 was absent in all analyzed species, and the gene ycf1 has been pseudo-genized in all Chinese chestnuts except C. pumlia. We analyzed the repeats and nucleotide substitutions in these plastomes and detected several highly variable regions. The phylogenetic analyses based on plastomes confirmed the monophyly of Castanea species.
One-Year Monitoring of Daily Earthworm Cast Production: Surface Cast Contribution to Soil Fertility in a Subtropical Forest
Authors: Wang Xiaoling , Fu Shenglei , Wang Xiaoli , Li Zhigang , Li Jianxiong , Zhang Weixin
Published: June 2021

The process of earthworm casting plays an important role in soil fertility. The contributions of earthworm casts to soil nutrients dynamics cannot be fully understood unless the temporal changes in earthworm cast production and the related nutrient resource pools are explored in the field. Here, we recorded the daily surface cast production of earthworms and monthly change patterns of carbon (C), nitrogen (N) and phosphorous (P) in casts in a subtropical plantation for one year and compared the nutrient concentrations and microbial community traits (structure and bacterial activity) in casts with those in bulk soils. Our objectives were to characterize the temporal patterns of earthworm cast production and the C and nutrients in casts and to assess the potential contribution of earthworm casts to soil fertility. The results showed that the annual surface cast production at the study site was 8.3 tons ha−1; monthly cast production was >80 g m−2 during April to November and <30 g m−2 during December to March. The annual outputs of total organic C (TOC), total N (TN) and total P (TP) in surface casts were 68.10, 4.84 and 0.38 g m−2, respectively; the annual outputs of available P (AP), NH4+-N and NO3−-N were 22.4, 147.0 and 61.3 mg m−2, respectively. The concentrations of TOC and TN in casts were higher during warm months, but NH4+-N was higher during cold months, while both the concentrations of TP and AP in casts were constant throughout the year. The nutrient concentrations were 1.6–4.3-fold greater, and the microbial biomass C and N contents were 7–8-fold greater in earthworm casts than those in bulk soils. However, the bacterial stress index (BSI) was significantly greater in casts than in the bulk soil, which implies that bacteria in casts were under stress. Accordingly, the ratio of fungal to bacterial biomass (F/B) was significantly higher in casts than in the bulk soil. Earthworm casting activity can process forest soil into a “new soil”, which forms large amounts of earthworm-induced hotspots of soil fertility. These resource pools in casts, with monthly fluctuated C and N and constant P, could be more readily accessed by fungi rather than bacteria. Earthworm casting activity potentially changes both the nutrients available and the interactions of plants and rhizosphere microbiota.
Genetic Evaluation and Combined Selection for the Simultaneous Improvement of Growth and Wood Properties in Catalpa bungei Clones
Authors: Xiao Yao , Wang Junhui , Yun Huiling , Yang Guijuan , Ma Jianwei , Ma Wenjun , Qu Guanzheng
Published: June 2021

Catalpa bungei is an important timber tree. Improvements in growth and wood quality are important goals of C. bungei breeding, and it is necessary to understand the genetic parameters of specific target traits and genetic correlation between growth traits and wood properties for tree breeding. In this study, the genetic parameters of height, diameter at breast height (DBH) and wood properties were estimated and genetic and phenotypic correlations between growth traits and wood properties were evaluated in C. bungei. Finally, different selection scenarios were used to evaluate and select optimal clones. The results showed that there were significant differences in growth and wood properties among clones. The wood hardness (0.66–0.79), basic density (0.89), air-dried density (0.89) and compression strength parallel to the grain of wood (CSP) (0.84) had high repeatability. The variance component proportions indicated that the variation in wood properties came mainly from different genotypes (clones) rather than from different individuals of the same clone. The DBH showed a significant negative genetic correlation with the hardness of radial section (HRS) (−643), basic density (−0.531) and air-dry density (−0.495). This unfavorable relationship makes it difficult to improve growth and wood quality simultaneously in C. bungei. We selected the optimal clones under different scenarios, and we obtained 7.75–9.06% genetic gains for growth in the scenario in which height and DBH were the target traits. Genetic gains of 7.43–14.94% were obtained for wood properties by selecting optimal clones in the scenario in which wood properties were the target traits. Approximately 5% and 4% genetic gains were obtained for growth and wood properties, respectively, for the combined selection. This study provides new insights into the genetic improvement of wood quality in C. bungei.
Increased Extracellular Saponin Production after the Addition of Rutin in Truffle Liquid Fermentation and Its Antioxidant Activities
Authors: Xu Ling , Su Qihui , Zhang Zhicai , Guan Guoqiang , Huang Daming , Terry Norman , Wang Feng
Published: June 2021

Saponins possess a variety of pharmacological effects and exhibit great potential in the food industry as bioactive substances. In this study, extracellular saponin production via the liquid fermentation of Tuber melanosporum occurred with the addition of rutin. For this purpose, medium composition and culture conditions were optimized using single-factor experiments and an orthogonal experiment design. The optimal medium consisted of glucose (43.5 g/L), peptone (6 g/L), KH2PO4 (1.15 g/L), NaCl (0.2 g/L), vitamin B2 (0.082 g/L), vitamin B6 (0.1 g/L), vitamin C (0.02 g/L), and rutin (4.8 g/L). The culture conditions were as follows: 12.5% (v/v) inoculation, medium volume of 50 mL/250 mL flask, culture temperature of 24 °C, shaker speed of 190 rpm, initial pH of 5.7, and culture time of 96 h. Finally, a maximal extracellular saponin content of 0.413 g/L was obtained, which was 134.7% higher than that in the base medium. Rutin proved to be an excellent promoter, because the saponin production was increased by 50.2% compared to that in the optimized medium without rutin. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, hydroxyl radical scavenging activity, and ferric reducing antioxidant power of truffle saponins reached 94.13%, 79.26%, and 42.22 mM, respectively. This study provides a useful strategy for fungal bioactive saponin production by liquid fermentation with the addition of flavonoid compounds.
Blending Proteins in High Moisture Extrusion to Design Meat Analogues: Rheological Properties, Morphology Development and Product Properties
Authors: Wittek Patrick , Karbstein Heike P. , Emin M. Azad
Published: June 2021

High moisture extrusion (HME) of meat analogues is often performed with raw materials containing multiple components, e.g., blends of different protein-rich raw materials. For instance, blends of soy protein isolate (SPI) and another component, such as wheat gluten, are used particularly frequently. The positive effect of blending on product texture is well known but not yet well understood. Therefore, this work targets investigating the influence of blending in HME at a mechanistic level. For this, SPI and a model protein, whey protein concentrate (WPC), were blended at three different ratios (100:0, 85:15, 70:30) and extruded at typical HME conditions (55% water content, 115/125/133 °C material temperature). Process conditions, rheological properties, morphology development, product structure and product texture were analysed. With increasing WPC percentage, the anisotropic structures became more pronounced and the anisotropy index (AI) higher. The achieved AI from the extrudates with a ratio of 70:30 (SPI:WPC) were considerably higher than comparable extrudates reported in other studies. In all extrudates, a multiphase system was visible whose morphology had changed due to the WPC addition. The WPC led to the formation of a much smaller dispersed phase compared to the overlying multiphase structure, the size of which depends on the thermomechanical stresses. These findings demonstrate that targeted mixing of protein-rich raw materials could be a promising method to tailor the texture of extruded meat analogues.
Quality Traits of Montanera Iberian Dry-Cured lomito as Affected by Pre-Cure Freezing Practice
Authors: Tejerina David , León Lucía , García-Torres Susana , Sánchez Miriam , Ortiz Alberto
Published: June 2021

The seasonality to which dry-cured products from Iberian breed pigs finished in Montanera (free-range rearing system with feed based exclusively on ad libitum consumption of natural resources; acorns and grass) are subjected could be overcome by pre-cure freezing. Three sets of Montanera Iberian presas (Serratus ventralis muscle) (n = 15) were established to assess the impact of frozen storage -0, or non-frozen, 3 and 6 months—previous to the technological process of curing—on the quality traits of the dry-cured product Montanera Iberian dry-cured lomito. Similar seasoning and curing processing conditions were applied to all sets. Lower productive performance due to higher weight loss during curing, and lower colour intensity were observed in pre-frozen dry-cured lomitos. The fatty acid profile was more saturated, and the oxidative status increased as a result of pre-cure freezing. On the matter of texture, all parameters were modified, highlighting the higher values of hardness and shear force of pre-frozen dry-cured lomitos. The time that raw material was frozen exerted a slight, thus helping manufacturers to better address the gap between industry and consumer demand with minimal effect on quality traits.
The Genetics of Parkinson’s Disease and Implications for Clinical Practice
Authors: Day Jacob Oliver , Mullin Stephen
Published: June 2021

The genetic landscape of Parkinson’s disease (PD) is characterised by rare high penetrance pathogenic variants causing familial disease, genetic risk factor variants driving PD risk in a significant minority in PD cases and high frequency, low penetrance variants, which contribute a small increase of the risk of developing sporadic PD. This knowledge has the potential to have a major impact in the clinical care of people with PD. We summarise these genetic influences and discuss the implications for therapeutics and clinical trial design.
Extracellular Vesicles and Host–Pathogen Interactions: A Review of Inter-Kingdom Signaling by Small Noncoding RNA
Authors: Stanton Bruce A.
Published: June 2021

The focus of this brief review is to describe the role of noncoding regulatory RNAs, including short RNAs (sRNA), transfer RNA (tRNA) fragments and microRNAs (miRNA) secreted in extracellular vesicles (EVs), in inter-kingdom communication between bacteria and mammalian (human) host cells. Bacteria secrete vesicles that contain noncoding regulatory RNAs, and recent studies have shown that the bacterial vesicles fuse with and deliver regulatory RNAs to host cells, and similar to eukaryotic miRNAs, regulatory RNAs modulate the host immune response to infection. Recent studies have also demonstrated that mammalian cells secrete EVs containing miRNAs that regulate the gut microbiome, biofilm formation and the bacterial response to antibiotics. Thus, as evidence accumulates it is becoming clear that the secretion of noncoding regulatory RNAs and miRNAs in extracellular vesicles is an important mechanism of bidirectional communication between bacteria and mammalian (human) host cells. However, additional research is necessary to elucidate how noncoding regulatory RNAs and miRNA secreted in extracellular vesicles mediate inter-kingdom communication.