bluuebunny/crossref_metadata_2025 huggingface dataset:
VIRTUAL WORLD TECHNOLOGIES IN MANAGING CHILDREN WITH LEARNING DISABILITY
Authors: Maral Priyaranjan , Prusty Anjana , Kiran Subba Usha
Published: November 2023

In technological era, virtual reality or augmented reality still remains
less popular within the fi eld of learning disabilities. Research shows
that children with learning disabilities face various challenges in their dayto-
day lives dealing with these disorders, demanding massive solutions. This
paper addresses the pros and cons of virtual reality in learning disabilities
across different age groups by combining theories of virtual worlds and
learning disorders. Exciting research in virtual reality focuses on fi nding out
how psychotherapies have benefi ts in learning and education. Upon review,
it becomes evident that research in the virtual world along with learning disabilities
has not yet been examined from a cohesive perspective, illustrating a
lack of alliance that determines a more global understanding of the technological
advantages of disabilities. Thus, this paper aims to provide educators
with an overview of explanations of the virtual world and to ensure appropriate
development of VR/AR applications and special assistance for learning
disabilities.
“UNITED IN PROBLEM SOLVING”. GAMIFICATION EXPERIENCES IN THE RENÉ FRAGA SCHOOL
Authors: Rodríguez Claudia Gómez
Published: November 2023

The education of our days faces an important challenge in the
enrichment of the teaching educational process: how to transmit the contents
in a fun way that generates interest and motivates the students. This
method called “gamifi cation” is another way of educating. It works with the
potential of students and makes learning an active process where the subject
is the protagonist. The present study is aimed at exploring the effect of the
gamifi cation method in the implementation of an educational program to develop
the ability to pose and solve problems in th grade children of the René
Fraga Primary School. It is a qualitative study, of an exploratory nature, since
investigations that inquire into the subject from this perspective is unknown.
We have as results that in spite of not having achieved a total assimilation of
the skill, the gamifi cation method motivated the students to work as a team
and contributed to obtain better results. Application of this method resulted
in a progressive increase in the quality of responses and in the development
of some of the skill indicators. It also generated a positive impact on the implementation
of the educational program.
INTERACTIVE DIGITAL EQUIPMENT IN THE PROGRAM OF COMMUNICATION SKILLS DEVELOPMENT FOR PRESCHOOLERS
Authors: Maltceva Alisa
Published: November 2023

The social situation of the development of modern children is signifi
cantly transformed due to large-scale digitalization. (Soldatova, Rasskazova,
Nestik). In this context, it becomes relevant to develop programs that are based
on modern technologies and contribute to environmental development. The goal
is to study the development of preschooler’s communication skills in using interactive
digital equipment. Research methods and sampling. The program was
developed for the “Interactive Sandbox” (LLC PlayStand). The study involved 
preschoolers aged -. Research methods included structured observation, according
to the criteria by A.M. Shchetinina, M.A. Nikiforova; drawing test by E.
Wartegg. Both techniques were carried out before and after the implementation
of the program. Main results showed that after the course, for all  observed
indicators, the scores increased by .-. points as compared to the fi rst measurement.
According to experts, the uneven development of the components
of communication skills became apparent: () the motivation to communicate
is higher than the communication skills themselves; () the cognitive sphere,
which provides the opportunity to build a game, describe and explain the plot, is
also better developed than communication skills. According to the Wartegg test,
the greatest positive dynamics was observed for four stimuli of the method: No.
 - projection of the self-concept, self-confi dence (% of children); No.  - projection
of the level of volitional regulation, achievement motivation (%); No.
 - projection of the desire for integration (%); No.  - projection of a sense
of inner security (%). The proposed program shows signifi cant effectiveness,
according to experts. However, the programs infl uenced intrapersonal characteristics
that can become the basis for further development of the communication
process - self-centering, a sense of security, motivation, the desire for integration
rather than the quality and intensity of communication, partner orientation, and
empathy.
TEACHER OF THE FUTURE SCHOOL AND DIGITAL TECHNOLOGIES: CONSOLIDATION OF OPPORTUNITIES
Authors: Mitina Larisa
Published: November 2023

Currently, there is no agreed understanding of the prospects for
the development of education and the place and role of the teacher in the
future. Scientifi c and educational communities, as well as the public, are divided
in opinion on what will be the basis for the school of the future: digital
technologies, competencies, achievements of sciences, personal orientation.
With different grounds for the prospects of education development, they can
mutually complement each other and be combined within the framework
of a systematic personality development approach. The purpose is to study
the personal and professional (including digital technologies) resources for
personal and professional development of a teacher, providing a set of conditions
for the development, upbringing, and training of students at the future
school. The study was conducted with a specially developed program,
including  methods, on a sample of teachers (n=) of Moscow schools.
Statistical methods included discriminant, regression, factor analysis. The results
of multiple regression analysis (MRA) showed that the main factors determining
the high level of personal and professional development in teachers
are personal resources: refl exive design, constructive coping, emotional
involvement. The technological components are identifi ed as additional. In
the new era of continuing education, the emphasis must inevitably shift from
the technological component to the personal one, including the values. The
teacher’s value system will include traditional cultural and educational values
(personality, interaction, creativity) as well as individual values, personal
and professional characteristics. At the same time, technological processes,
including digitalization, will expand and complement the teacher’s resource
capabilities for the development of students at the school of the future.
USING A SMART SPEAKER IN THE EDUCATIONAL PROCESS OF PRESCHOOLERS
Authors: Polyakova Alexandra
Published: November 2023

Introduction of modern technologies, namely smart speakers, in
preschool education is gaining relevance. The purpose of the study is to develop
methodological material for teachers and parents on the use of “speakers”
in all educational fi elds. Research methods include questionnaire, comparison,
analysis, and observation. Sample includes parents (n=) and
teachers (n=). When analyzing the parents’ questionnaire, it turned out
that more than half of the respondents use speakers at home, but not all of
them know what range of games the system offers. At the same time, .%
of parents support the use of modern technologies within the preschool educational
organization. % of respondents consider the introduction of a
smart speaker in kindergarten possible. When analyzing the questionnaire
of teachers, it turned out that % of respondents would like to use a smart
column in classroom but only % know how to use it. The use of smart
speakers in preschool organizations will undoubtedly affect the quality of
education and development of the child.
ORIENTING BASIS FOR CHOOSING CLASSROOM TACTICS FOR STUDENTS AT EDUCATIONAL PRACTICE
Authors: Pshenichnyuk Diana , Yakushina Anastasia
Published: November 2023

Educational practice is an important part of professional training of
students in many specialties. The live pedagogical process is diffi cult for trainee
students. Those who have no experience in teaching or speaking in front of the
audience worry a lot. Supervisors of pedagogical practice for students, particularly
psychology students, in both Russia and Azerbaĳ an are convinced of this.
The authors of the article faced these problems in -. To solve them, we
developed an algorithm for selecting the tactics of teacher-practitioner (student-
practitioner) behaviour according to the audience factor in traditional
face-to-face teaching. The algorithm is also applicable to online classroom format.
Algorithm of work tactics choice by the factor “audience composition and
structure”: . Diagnose the structure of the audience by the factors of homogeneity,
size, level of preparation. . Identify the presence/absence of a leader
and/or a marginalised person in the audience. . Identify (fi nd) the “backbone”
part of the audience. . Choose and stick to the tactics according to results of
diagnostics. Following the rules of self-control. . Form and maintain the favorable
climate in the audience, productive atmosphere of work. . Make conclusions
at the end of the class-teaching about the productivity of the chosen
tactics of behaviour. The algorithm is based on the principle of an indicative
action framework. The “A” - action - is the action of choosing the tactics of the
teacher-practitioner’s behaviour according to the factors of the classroom characteristics.
The factors include size, homogeneity, profi ciency level, and general
mood of the participants. The presented algorithm was implemented in
the content of students’ preparatory work before pedagogical practice in -
. Students applied this algorithm to choose strategies for their behaviour
in practice. The authors of the article received acknowledgements from student
practitioners who emphasised the effectiveness of the algorithm. However, the
authors did not take separate measurements, so this text is offered as a theoretical
model for students’ choice of tactics in their pedagogical practice.
NEW TECHNOLOGIES IN SOCIAL INCLUSION OF CHILDREN WITH DISABILITIES
Authors: Ceballos Danis Rodríguez , Sánchez Sara María Berrío , Betancourt Yeline Ruiz
Published: November 2023

Although the social inclusion of boys and girls with disabilities
has methodological, academic and even legal requirements, well-defi ned
demands for Cuban society, they manifest themselves in their local leaders,
and social representations that impede the process. The use of new information
technologies in a training strategy in this regard is a valuable alternative
and constitutes the relevance of this research. The general objective of this
research is to demonstrate the opportunity and relevance of the use of new
technologies in the development of a training strategy that contributes to
the empowerment on social inclusion of boys and girls with disabilities in
local leaders of Cuban community. It constitutes a qualitative research of the
action - research type supported by theoretical, historical, logical, analytical,
synthetic, inductive, deductive, systemic, structural, and modeling methods
as well as those of the empirical level such as observation, ethnographic survey,
consultations with specialists, and discussion group. As a population, 
local leaders were selected in charge of contributing to the social inclusion of
boys and girls with disabilities. As a result, a training strategy emerges that,
based on the advantages of the use of new technologies, makes it possible for
each action not only to transform reality, but also to get the protagonists involved.
Its fl exible and cyclical nature allowed adaptability in the process and
the possibility to incorporate other knowledge. By way of conclusions, it was
determined that the strategy is a valuable tool in the face of current demands
for the elimination of gaps and inequities from the community context. From
the perspectives of the theory of social representations, it guarantees a new
way of projecting the training of change managers towards the social inclusion
of girls and boys with disabilities.
ON THE DISTANCE EDUCATION OF CHILDREN AND THE UNRESOLVED ISSUES IN E-LEARNING
AT SCHOOL
Authors: Suvorova Galina
Published: November 2023

The development of psychological science has led to the development
of artifi cial intelligence and distance learning for children, which
require development of all cognitive abilities, thinking, and intelligence in
students. The introduction of digital technologies in the educational process
is not based on the examination results on the material of school textbooks.
What goals do digital technologies teach students? What specifi c and abstract
concepts are included in the content of education? Computer use leads
to the question of quality criteria for education. There is no school learning
computerization model that is based on the psychological analysis of educational
activities and students’ abilities. A child in any environment learns,
develops and is brought up by adults. A child can learn being a functional
psychological system of activity (Shadrikov). Rubinstein showed the connection
between thinking and mental abilities. Elkonin identifi ed the learning
goal in the learning task and learning actions to which he attributes actions
with objects and cognitive actions. Systems of cognitive actions characterize
the operational mechanisms of abilities. Shadrikov singled out a universal
operating mechanism of abilities – intellectual operations. We need methods
of psychological analysis for educational activities and the ability to learn,
the ability to desire and want, the ability to feel and experience.
AUDIOVISUAL CONSUMPTION IN EARLY CHILDHOOD DEVELOPMENT. A LOOK FROM CUBA
Authors: Pérez Annia Cano
Published: November 2023

There are many national and international investigations that
recognize the important role that audiovisuals play in the development of the
human being, as well as the need for the consumption activity to be mediated,
guided by others. However, few are those that provide a look at audiovisual
consumption from early childhood, but fewer are those that do so with a potentializing
vision on development. In a general sense, the exclusion of early
childhood is not surprising, since this behavior is similar in other spheres of
action or areas of study, where the fi rst years of the life of girls and boys (in
addition, those of greater signifi cance for current and future development),
are subsumed or made invisible. This happens within a more encompassing
denomination such as childhood, which, by including, also generalizes girls
and boys, depriving them of the consideration of particularities and peculiarities,
making them not only specifi c but also different. The exposed constitute
reasons to locate the Cuban child of early childhood in the center of
the investigations and to reveal the particularities that audiovisual consumption
acquires from the dimensions. The conception of child development, determination
of audiovisual content, exercise of mediation, management of
audiovisual content and carrier devices, as well as sociocultural context, its
components and indicators are regarded. This approach transcends the practices
and consumption habits traditionally studied in other age groups. Being
able to identify characteristic features of this process in the specifi c context
of Cuba not only constitutes a valuable contribution of the social sciences to
the development of more accurate public policies, but also aims to contribute
to the improvement of early childhood education that is developed. Likewise,
it opens a way to settle debts from the sciences with an age period that decisively
marks the future life of individuals, society and the nation. The focus
of the study carried out is fundamentally qualitative, in which the theoretical,
analytical-synthetic, inductive-deductive and systematization methods
of scientifi c research are privileged.
MANAGING THE PROCESS OF FORMATION OF THE CARIBBEAN CULTURAL IDENTITY IN THE HIGHER EDUCATION INSTITUTIONS
Authors: Oliva Paulina Marlen Casañas , Murguia Wendy Rodríguez
Published: November 2023

In nowadays society, the importance of knowing who we are
and of where we go is essential to face the globalisation. It is in the teaching
and learning process that professors can contribute to the formation of
values, beliefs, customs which are a part of the cultural identity that allows
self-recognition and recognizing the cultural diversity of the world we live
in. The cultural identity is seen as a social necessity that goes beyond educational
process and the formation of professional. According to this basis,
the investigation is focused on the management of the process of formation
the Caribbean cultural identity in students of the English Language career.
To carry out this investigation several methods were applied, among them,
the analysis - synthesis, the induction- deduction, observation, interview to
get to know the core of the management in forming the Caribbean cultural
identity and the current level of the variable. The investigation in progress
favors the correct management of the Caribbean cultural identity taking into
account the four dimensions of educative management: institutional, administrative,
pedagogical, and extensions in the four components of the process.
As a result of this investigation the content of Caribbean cultural identity has
been incorporated in the strategy of career as well as in the academic year.
Putting into practice a correct management of the Caribbean cultural identity
allows to provide for high quality education, supports the students with a
solid identity to face the digital era and globalization.
MEDIA SOCIALIZATION OF RUSSIAN YOUTH THROUGH NATIONAL DIGITAL PLATFORMS: FROM CONSENSUS AND “NEW PATRIOTISM” TO THE NOSTALGIA EFFECT
Authors: Dunas Denis , Babyna Dariana
Published: November 2023

The paper examines the issue of media socialization as a process of
assimilation by representatives of the media audience of socio-cultural norms
and values dominated in society. The authors try to consider media socialization
as a complex and heterogeneous process that can be decomposed into
three elements depending on the predominance of a media usage model. Firstly,
the media act as an agent of secondary socialization, which corresponds to
the basic normative understanding of media functioning in society. With this
understanding, the media cannot form the basic values of a young audience,
since they are not able to compete with “signifi cant others”: parents, peers,
schoolteachers, who are traditionally assigned the role of the most infl uential
agents of primary socialization. Secondly, the media can act as an agent of
self-socialization, that is, participate in the process of independent choice of
media channels and media resources to develop the diversifi ed interests of the
audience. Thirdly, the media can act as an agent of primary socialization and
displace the infl uence of “signifi cant others”. The article presents the result of
the empirical research in which the process of agenda setting of youth communities
in the VK social media platform were studied (“Rhymes and Punches”,
“Leonardo Dayvinchik”, “Oatmeal, sir”). The study drew conclusions about the
complex structure of a process of digital media socialization. It was found that
the content corresponds to traditional Russian spiritual values, while the form
corresponds to the global ones. The result of the study was the recognition of
the simultaneity of the implementation for all types of media socialization in
general, the impossibility to strictly distinguish one element from another, and
at the same time, a pronounced predominance of any type of media socialization
through diverse digital content. The study was funded by a grant from the
Russian Science Foundation (Project No. --).
Penerapan Akad Murabahah Pada Pembiayaan Griya Takeover (Studi Kasus di Bank Syariah Indonesia KCP Kuta)
Authors: Nur Halimah  , Kurniawati Kurniawati  , Ely Mansur
Published: November 2023

The aims of this study are, to find out the factors that lead to a murabahah contract in the financing of a takeover griya at BSI KCP Kuta, to find out the murabaahah contract process for financing a takeover house at BSI KCP Kuta and, to find out the process of financing the griya takeover at BSI KCP Kuta. The research method used is a descriptive qualitative method with data collection techniques through observation, interviews, and documentation. The data analysis technique used is the Miles and Huberman model, which includes data collection, data reduction, data presentation, and data verification. data validity technique with the triangulation technique. The results of the study found, there are factors that cause a murabaha contract to occur, namely the presence of the object of the contract. The murabaha contract process by submitting asset documents to the consumer as a sign of consent qabul with the consumer. And financing for takeover houses that are free from usury in accordance with the Fatwa of the National Sharia Council, MUI No.04/DSN-MUI/IV/2000, dated April 1, 2000, conceming murabaha. The process of financing takeover housing includes BSI having the terms and benefits of financing takeover housing. To attract consumer, as well as with light installments, one of the benefits of takeover financing at BSI is that consumers still feel able to meet other needs.
Implementasi Budaya Organisasi dalam Meningkatkan Produktivitas Kerja di Bank Muamalat Kantor Cabang Denpasar
Authors: Nur Azizah  , Ely Mansur  , Sahrial Sahrial
Published: November 2023

The objectives of this research are: 1). To find out the implementation of organizational culture in increasing work productivity at Bank Muamalat Denpasar Branch Office. 2). To find out how each employee increases work productivity at Bank Muamalat Denpasar Branch Office? The research methodology used is descriptive qualitative research using triangulation in testing credibility. The informants in this study were 6 employees who worked at Bank Muamalat Denpasar Branch Office. As well as data collection techniques in the form of: interviews, documentation and observation. Based on the results of the research, Bank Muamalat Denpasar Branch Office has implemented ideal corporate culture values properly and correctly. Where the organizational culture is applied as motivation for each employee to be more disciplined and have character in the application of organizational culture when carrying out their duties. The application of organizational culture can increase the work productivity of employees which can be seen from the achievements of the company. So that the organizational culture applied so far has been running well and in accordance with sharia principles.
An Overview of The Level of Occupational Stress of Anesthesiologists In The Surgical Room of The Hospital In The Banyumas District Area
Authors: Assyifa Ibnu , Sukmaningtyas Wilis , Burhan Asmat
Published: November 2023

Background: Every profession and employee in both developed and developing countries is affected by occupational stress, which is a global problem. Due to the great burden and responsibility of safeguarding human lives, the anesthesia care profession is particularly vulnerable to stress. And high levels of occupational stress can cause health problems, such as mental and behavioral disorders such as fatigue, anxiety, and depression, as well as other physical disorders such as cardiovascular disease and musculoskeletal disorders that affect service functions. The role of expert anesthesiologist has a function in pre, intra, post anesthesia care services and emergency management. Objective: To identify the characteristics of anesthesiologist's work stress level based on age, gender, working period, working hours and latest education in the surgical room. Methods: This research is descriptive quantitative with a cross sectional approach. The study population was 39 respondents, the focus of observation in this study was a description of the stress level of anesthesia administrators in the surgical room of the Banyumas Regency Hospital. Results: This study shows the level of stress with mild stress category with a total of 19 respondents with a percentage (48.7%) and moderate work stress as many as 4 respondents with a percentage (10.3%). Conclusion: The results of this study indicate the presence of mild and moderate work stress in surgical room anesthesiologists at the Banyumas Regency Regional Hospital.
EFFECT OF LISTENING TO ASMAUL HUSNA ON ANXIETY OF PREOPERATIVE PATIENTS IN RSI PURWOKERTO
Authors: Wahyuni Dwi , Sukmaningtyas Wilis , Burhan Asmat
Published: November 2023

Background: Surgery is a medical treatment that uses invasive techniques by making an incision on the body part to be treated and ends with wound closure and suturing. This can cause both physiological and psychological stress. The psychological response to stress can be anxiety. This occurs because of the fear of bad possibilities that will happen that will harm the patient himself. Purpose: Knowing the effect of listening to Asmaul Husna on anxiety of preoperative patients at RSI Purwokerto. Methods: Design quasy experiment using Pretest-Posttest Control Group Design with sampling technique using purposive sampling technique as many as 116 respondents. The instrument used the Hamilton Anxiety Rating Scale (HARS). Data analysis using the Mann Whitney-U test. Results: We confirmed the effect of Asmaul Husna on anxiety. Paired T-test analysis of the control group with z= -0.37 with p = 0.70, while in the experimental group the mean ± SD was 0.27 ± 0.48 (CI 95% 0.14, 0.40) with p = 0.000. Mann Whitney-U analysis showed a value of z = -2.927, with p = 0.003. This shows that there is an effect of listening to Asmaul Husna on reducing the anxiety of preoperative patients at RSI Purwokerto. Conclusions: there is an effect of listening to Asmaul Husna on reducing anxiety of preoperative patients at RSI Purwokerto.
Overview of the Incidence of Hypotension After Spinal Anesthesia Induction in Sectio Caesarea Patients During Intra Anesthesia at Juanda Kuningan Hospital West Java
Authors: Hidayatullah Riki , Lintang Suryani Roro , Dewi Pramesti
Published: November 2023

Purpose: This study aimed to identify the incidence of hypotension after spinal anesthesia induction in SC patients during intra-anesthesia based on Body Mass Index (BMI), age, and baseline systolic blood pressure at Juanda Kuningan Hospital. Methods: This study used a quantitative descriptive method with a cross sectional approach. The sample in this study were all SC patients with spinal anesthesia techniques who met the criteria. The sampling technique used was purposive sampling with the slovin formula resulting in 90 respondents. And data analysis using univariate analysis. Findings: The results showed that the majority of respondents who experienced mild hypotension with excessive BMI status were 50 respondents (58.8%), the majority of respondents who experienced mild hypotension with early adulthood were 52 respondents (61.2%) and the majority of respondents who experienced mild hypotension with initial systolic blood pressure were at initial systolic blood pressure <120 mmHg and 120-130 mmHg as many as 31 respondents (36.5%).  Conclusions: The results of this study illustrate that the majority of respondents will experience hypotension after spinal anesthesia induction. This is because spinal anesthesia can block sympathetic nerves, resulting in a decrease in circulating arterial tone, this condition causes peripheral arterial vasodilation. This vasodilating effect can result in a decrease in vascular resistance (hypotension). For further researchers to be able to develop this study to look for other variables that may be associated with the incidence of hypotension after induction of spinal anesthesia in SC patients so that the results of the study will show more variation.
Universitetspædagogik i oversigt: Begreber og metoder
Authors: Lindberg Annika Büchert
Published: November 2023

Rienecker, L. & Troelsen, R (2023) Universitetspædagogik i oversigt: Begreber og metoder. Samfundslitteratur
I opslagsbogen ’Universitetspædagogik i oversigt: Begreber og metoder’ kan du som underviser, uddannelsesansvarlig eller medarbejder i studieadministrationen hurtigt få en introduktion til eller en forklaring på de nøglebegreber, som anvendes i forbindelse med udvikling og kvalitetsarbejde af universiteternes uddannelser. Bogens korte kapitler formår både at rammesætte begreberne samt at nuancere og eksemplificere dem, hvilket gør dem yderst brugbare som oplæg til diskussioner i forskellige fora. Hvert kapitel inspirerer desuden til både refleksion og yderligere læsning.
Opportunities to Use the Local Community’s Potential to Ensure Sustainable Regional Development in Latvia
Authors:   , Pudzis Edgars
Published: November 2023

The Doctoral Thesis is a set of scientific articles providing a summary of the author’s research. With the set of scientific articles, the author has substantiated the course of the research, the interconnection of separate articles, as well as the thematic unity and sequence of the research. Description of concepts such as village, community and smart village, have been analysed. The process of involving a local community is analysed, with examples of successful involvement of the community, as well as the village development assessment indicators are developed. Also, a proposal for the local territory/community development planning model has been created in this paper.
Social/ economic burden and health-related quality of life in patients with Spinal Muscular Atrophy (SMA) in Greece
Authors: Kontogiannis Vasileios , Loupas Marios Athanasios , Adamopoulou Mary , Athanasiou Dimitris , Moraiti Georgia , Traeger-Synodinos Jan , Sofocleous Chrystallena , Kekou Kyriaki , Kalogeropoulou Maria
Published: November 2023

Objective: The objective of this study was to determine the economic burden and health-related quality of life (HRQoL) of patients with SMA and their caregivers in Greece, which is still unknown, and explore the economic impact of the implementation of a reproductive carrier screening (RCS) program. Methods: A cross-sectional study was conducted in which an online questionnaire was completed by caregivers of SMA patients. A cost of illness (COI) model was developed in which costs per patient were calculated from a societal perspective and extrapolated to the Greek SMA population. Parents’/caregivers’ HRQoL was measured using the 36-item PedsQL™ Family Impact Module. Resources for the implementation of RCS program were estimated and its short-term impact was assessed. Results: The COI was estimated at €261,785 per patient in 2022. When extrapolating this across all affected persons, the average annual cost ranged from €49,739,395 to €91,036,017 depending on the prevalence considered. Out-of-pocket expenses accounted for 8% of the total. Results of the analysis on HRQoL indicated a high burden on parents/ caregivers with the majority of scores falling below 50 and a mean total score of 45. Depending on the scenario used, an RCS program could save the Greek healthcare system at least €167,181 or increase its costs by €564,804. Conclusions: SMA imposes a significant economic burden in affected patients, caregivers, and health care system. The cost-saving magnitude of a potential implementation of a CRS program is dependent on the parents' reproductive choices following a positive screening result.
Bestrophin-4 relays Hes4 and interacts with Twist1 to suppress epithelial-to-mesenchymal transition in colorectal cancer cells
Authors: Wang Zijing , Xia Bihan , Qi Shaochong , Zhang Xian , Zhang Xiaoshuang , Li Yan , Wang Huimin , Zhang Miao , Zhao Ziyi , Kerr David , Yang Li , Cai Shijie , Yang Jinlin
Published: November 2023

Bestrophin isoform 4 (
BEST4
) is a newly identified subtype of the calcium-activated chloride channel family. Analysis of colonic epithelial cell diversity by single cell RNA-sequencing has revealed the existence of a cluster of
BEST4
+ mature colonocytes in humans. However, if the role of
BEST4
is involved in regulating tumour progression remains largely unknown. In this study, we demonstrate that
BEST4
overexpression attenuates cell proliferation, colony formation, and mobility in colorectal cancer (CRC)
in vitro
, and impedes the tumor growth and the liver metastasis
in vivo
.
BEST4
is coexpressed with hairy/enhancer of split 4 (
Hes4
) in the nucleus of cells, and
Hes4
signals
BEST4
by interacting with the upstream region of the
BEST4
promoter.
BEST4
is epistatic to
Hes4
and downregulates Twist1, thereby inhibiting epithelial-to-mesenchymal transition (EMT) in CRC. Conversely, knockout of BEST4 using CRISPR/Cas9 in CRC cells revitalises tumor growth and induces EMT. Furthermore, the low level of the
BEST4
mRNA is correlated with advanced and the worse prognosis, suggesting that
BEST4
functions as a tumor suppressor in CRC.
Rab7 dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis
Authors: Gaur Preksha , Rajendran Yesheswini , Srivastava Bhagyashree , Markandey Manasvini , Fishbain-Yoskovitz Vered , Mohapatra Gayatree , Suhail Aamir , Chaudhary Shikha , Tyagi Shaifali , Yadav Subhash C , Pandey Amit K , Merbl Yifat , Bajaj Avinash , Ahuja Vineet , Srikanth Chittur V
Published: November 2023

Inflammation in ulcerative colitis is typically restricted to the mucosal layer of distal gut. Disrupted mucus barrier coupled with microbial dysbiosis has been reported to occur prior to the onset of inflammation. Here, we show the involvement of vesicular trafficking protein Rab7 in regulating the colonic mucus system. We identified a lowered Rab7 expression in goblet cells of colon during human and murine colitis.
In vivo
Rab7 knocked down mice (Rab7
KD
) displayed a compromised mucus layer, increased microbial permeability and depleted gut microbiota with enhanced susceptibility to dextran sodium-sulfate induced colitis. These abnormalities emerged owing to altered mucus composition, as revealed by mucus proteomics, with increased expression of mucin protease Chloride channel accessory 1 (CLCA1). Mechanistically, Rab7 maintained optimal CLCA1 levels by controlling its lysosomal degradation, a process that was dysregulated during colitis. Overall, our work establishes a role for Rab7 dependent control of CLCA1 secretion required for maintaining mucosal homeostasis.
Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing is associated with therapeutic response to splicing inhibitor
Authors: Jablonowski Carolyn , Quarni Waise , Singh Shivendra , Tan Haiyan , Bostanthirige Dhanushka Hewa , Jin Hongjian , Fang Jie , Chang Ti-Cheng , Finkelstein David , Cho Ji-Hoon , Hu Dongli , Pagala Vishwajeeth , Sakurada Sadie Miki , Pruett-Miller Shondra M. , Wang Ruoning , Murphy Andrew , Freeman Kevin , Peng Junmin , Davidoff Andrew M , Wu Gang , Yang Jun
Published: November 2023

Dysregulated pre-mRNA splicing and metabolism are two hallmarks of MYC-driven cancers. Pharmacological inhibition of both processes has been extensively investigated as potential therapeutic avenues in preclinical and clinical studies. However, how pre-mRNA splicing and metabolism are orchestrated in response to oncogenic stress and therapies is poorly understood. Here, we demonstrate that JMJD6 acts as a hub connecting splicing and metabolism in MYC-driven neuroblastoma. JMJD6 cooperates with MYC in cellular transformation by physically interacting with RNA binding proteins involved in pre-mRNA splicing and protein homeostasis. Notably, JMJD6 controls the alternative splicing of two isoforms of glutaminase (GLS), namely kidney-type glutaminase (KGA) and glutaminase C (GAC), which are rate-limiting enzymes of glutaminolysis in the central carbon metabolism in neuroblastoma. Further, we show that JMJD6 is correlated with the anti-cancer activity of indisulam, a “molecular glue” that degrades splicing factor RBM39, which complexes with JMJD6. The indisulam-mediated cancer cell killing is at least partly dependent on the glutamine-related metabolic pathway mediated by JMJD6. Our findings reveal a cancer-promoting metabolic program is coupled with alternative pre-mRNA splicing through JMJD6, providing a rationale to target JMJD6 as a therapeutic avenue for treating MYC-driven cancers.
Menopause, Brain Anatomy, Cognition and Alzheimer’s Disease
Authors: Costantino Manuela , Pigeau Grace , Parent Olivier , Ziolkowski Justine , Devenyi Gabriel A. , Gervais Nicole J. , Mallar Chakravarty M.
Published: November 2023

The menopause transition has been repeatedly associated with decreased cognitive performance and increased incidence of Alzheimer’s Disease (AD), particularly when it is induced surgically
1,2
or takes place at a younger age
3,4
. However, there are very few studies that use neuroimaging techniques to examine the effects of these variables in aggregate and in a large sample. Here, we use data from thousands of participants from the UK Biobank to assess the relationship between menopausal status, menopause type (surgical or natural), and age at menopause with cognition, AD, and neuroanatomical measures derived from magnetic resonance imaging. We find that for brain and cognitive measures, menopausal status, menopause type and age at surgical menopause do not impact the brain; but that there is a positive correlation between anatomy, cognition and age at non-surgical menopause. These results do not align with previous reports in the literature with smaller samples. However, we confirm that both early and surgical menopause are associated with a higher risk of developing AD, indicating that early and abrupt ovarian hormone deprivation might contribute to the development of the disorder.
Deficiency of orexin receptor type 1 in dopaminergic neurons increases novelty-induced locomotion and exploration
Authors: Xiao Xing , Yeghiazaryan Gagik , Eggersmann Fynn , Cremer Anna Lena , Backes Heiko , Kloppenburg Peter , Hausen Anne Christine
Published: November 2023

Orexin signaling in the ventral tegmental area and substantia nigra promotes locomotion and reward processing, but it is not clear whether dopaminergic neurons directly mediate these effects. We show that dopaminergic neurons in these areas mainly express orexin receptor subtype 1 (Ox1R). In contrast, only a minor population in the medial ventral tegmental area express orexin receptor subtype 2 (Ox2R). To analyze the functional role of Ox1R signaling in dopaminergic neurons, we deleted Ox1R specifically in dopamine transporter-expressing neurons of mice and investigated the functional consequences. Deletion of Ox1R increased locomotor activity and exploration during exposure to novel environments or when intracerebroventricularely injected with orexin A. Spontaneous activity in home cages, anxiety, reward processing, and energy metabolism did not change. Positron emission tomography imaging revealed that Ox1R signaling in dopaminergic neurons affected distinct neural circuits depending on the stimulation mode. In line with an increase of neural activity in the lateral paragigantocellular nucleus (LPGi) of Ox1RΔDAT mice, we found that dopaminergic projections innervate the LPGi in regions where the inhibitory dopamine receptor subtype D2 but not the excitatory D1 subtype resides. These data suggest a crucial regulatory role of Ox1R signaling in dopaminergic neurons in novelty-induced locomotion and exploration.
Deficiency of Orexin Receptor Type 1 in Dopaminergic Neurons Increases Novelty-Induced Locomotion and Exploration
Authors: Xiao Xing , Yeghiazaryan Gagik , Cremer Anna L. , Backes Heiko , Kloppenburg Peter , Hausen A. Christine
Published: November 2023

Orexin signaling in the ventral tegmental area and substantia nigra promotes locomotion and reward processing, but it is not clear whether dopaminergic cells directly mediate these effects. We show that dopaminergic neurons in these areas mainly express orexin receptor subtype 1 (Ox1R). In contrast, only a minor population in the medial ventral tegmental area express orexin receptor subtype 2 (Ox2R). To analyze the functional role of Ox1R signaling in dopaminergic neurons, we deleted Ox1R specifically in dopamine transporter-expressing neurons of mice and investigated the functional consequences. The deletion of Ox1R increased locomotor activity and exploration during exposure to novel environments or when intracerebroventricularely injected with orexin. Spontaneous activity in home cages, anxiety, reward processing, and energy metabolism did not change. Positron emission tomography imaging revealed that Ox1R signaling in dopaminergic neurons affected distinct neural circuits depending on the stimulation mode. In line with an increase of neural activity in lateral paragigantocellular nucleus (LPGi) of Ox1R
ΔDAT
mice, we found that dopaminergic projections innervate the LPGi in regions where the inhibitory dopamine receptor subtype D2 but not the excitatory D1 subtype resides. These data suggest a crucial regulatory role of Ox1R signaling in dopaminergic neurons in novelty-induced locomotion and exploration.
The molecular basis of Abelson kinase regulation by its αI-helix
Authors: Paladini Johannes , Maier Annalena , Habazettl Judith Maria , Hertel Ines , Sonti Rajesh , Grzesiek Stephan
Published: November 2023

Abelson tyrosine kinase (Abl) is regulated by the arrangement of its regulatory core, consisting sequentially of the SH3, SH2 and kinase (KD) domains, where an assembled or disassembled core corresponds to low or high kinase activity, respectively. It was recently established that binding of type II ATP site inhibitors, such as imatinib, generates a force from the KD N-lobe onto the SH3 domain and in consequence disassembles the core. Here, we demonstrate that the C-terminal αI-helix exerts an additional force towards the SH2 domain, which correlates both with kinase activity and type II inhibitor-induced disassembly. The αI-helix mutation E528K, which is responsible for the ABL1 malformation syndrome, strongly activates Abl by breaking a salt bridge with the KD C-lobe and thereby increasing the force onto the SH2 domain. In contrast, the allosteric inhibitor asciminib strongly reduces Abl’s activity by fixating the αI-helix and reducing the force onto the SH2 domain. These observations are explained by a simple mechanical model of Abl activation involving forces from the KD N-lobe and the αI-helix onto the KD/SH2SH3 interface.
Genome-wide identification of CaARR-Bs transcription factor gene family in pepper and their expression patterns under salinity stress
Authors: Alshegaihi Rana M. , Alshamrani Salha Mesfer
Published: November 2023

In plants, ARRs-B transcription factors play a crucial role in regulating cytokinin signal transduction, abiotic stress resistance, and plant development. A number of adverse environmental conditions have caused severe losses for the pepper (Capsicum annuum L.)—a significant and economically important vegetable. Among the transcription factors of the type B-ARRs family, multiple members have different functions. In pepper, only a few members of the ARRs-B family have been reported and characterized. The current study aimed to characterize ARRs-B transcription factors in C. annuum, including phylogenetic relationships, gene structures, protein motif arrangement, and RT-qPCR expression analyses and their role in salinity stress. In total, ten genes encode CaARRs-B transcription factors (CaARR1 to CaARR10) from the largest subfamily of type-B ARRs were identified in C. annum. The genome-wide analyses of the CaARRs-B family in C. annuum were performed based on the reported ARRs-B genes in Arabidopsis. An analysis of homologous alignments of candidate genes, including their phylogenetic relationships, gene structures, conserved domains, and qPCR expression profiles, was conducted. In comparison with other plant ARRs-B proteins, CaARRs-B proteins showed gene conservation and potentially specialized functions. In addition, tissue-specific expression profiles showed that CaARRs-B genes were differentially expressed, suggesting functionally divergent. CaARRs-B proteins had a typical conserved domain, including AAR-like (pfam: PF00072) and Myb DNA binding (pfam: PF00249) domains. Ten of the CaARRs-B genes were asymmetrically mapped on seven chromosomes in Pepper. Additionally, the phylogenetic tree of CaARRs-B genes from C. annuum and other plant species revealed that CaARRs-B genes were classified into four clusters, which may have evolved conservatively. Further, using quantitative real-time qRT-PCR, the study assessed the expression patterns of CaARRs-B genes in Capsicum annuum seedlings subjected to salt stress. The study used quantitative real-time qRT-PCR to examine CaARRs-B gene expression in Capsicum annuum seedlings under salt stress. Roots exhibited elevated expression of CaARR2 and CaARR9, while leaves showed decreased expression for CaARR3, CaARR4, CaARR7, and CaARR8. Notably, no amplification was observed for CaARR10. This research sheds light on the roles of CaARRs-B genes in pepper’s response to salinity stress. These findings enrich our comprehension of the functional implications of CaARRs-B genes in pepper, especially in responding to salinity stress, laying a solid groundwork for subsequent in-depth studies and applications in the growth and development of Capsicum annuum.
An evaluation of biomotor characterstics according to biological maturation level in 11-12 age soccer players
Authors: Aydin Serkan
Published: November 2023

The aim of this study was to evaluate the biomotor characteristics of 11–12-year-old soccer players according to their biological maturation level. The participants in the study were divided into 3 groups according to their peak height (PHV) values which determine their biological maturation levels. The mean age, height and body mass of the participants were 11.22±0.42 years, 163.56±9.30 cm, 54.37±10.05 kg for the PHV1 group, 11.46±0.55 years, 152.45±5.22 cm, 41.32±4.84 kg for the PHV2 group, 11.10±0.31 years, 148.60±4.42 cm, 39.21±4.59 kg for the PHV3 group. The participants were competitive athletes with at least 2 years of continuous training and competition experience. The maturation level of the participants, was determined by Mirwald equation. Biomotor characteristics of the participants were determined by hand grip strength and back leg strength, standing long jump, flexibility, yo-yo intermittent recovery tests. Data were analyzed using IBM SPSS 26 package program. One-way analysis of variance (ANOVA) was used for comparison between groups and Tukey HSD test was used for Post Hoc comparisons of significant relationships. As a result of the evaluation made according to the biological maturation levels of the participants, numerically positive effects on biomotor characteristics were found as the biological maturation level increased. A statistically significant difference between the data constituting the positive effects was observed only in the hand grip strength data. According to the Tukey multiple comparison analysis, it was determined that this significant difference occurred as the biological maturation level increased. As a result of the data obtained as a result of our research, it was concluded that the biomotor characteristics of 11-12 age group soccer players developed positively according to the level of biological maturation. In line with these results, it can be stated that biological maturation has a positive effect on biomotor characteristics.