bluuebunny/crossref_metadata_2025 huggingface dataset:
Very high cycle fatigue properties of 2000 MPa ultrahigh-strength steels
Authors: Yu Wenchao , Zhu Shipeng , He Xiaofei , Wang Maoqiu
Published: April 2022

The microstructure, mechanical properties and very high cycle fatigue (VHCF) properties of two 2000 MPa ultrahigh-strength martensitic steels were investigated. Results show that the newly developed steel possesses lower ultimate tensile strength but higher yield strength compared with the conventional steel, as well as a higher impact toughness and fracture toughness due to its better cleanliness and tempering at a higher temperature. Both steels experienced a typical VHCF fracture process. The newly developed steel had higher fatigue strength and longer fatigue life at the same stress amplitude compared with the conventional one, which could be related to its lower crack growth rate and better toughness.
Lineage tracing clarifies the cellular origin of tissue-resident macrophages in the developing heart
Authors: Liu Kuo , Jin Hengwei , Tang Muxue , Zhang Shaohua , Tian Xueying , Zhang Mingjun , Han Ximeng , Liu Xiuxiu , Tang Juan , Pu Wenjuan , Li Yan , He Lingjuan , Yang Zhongzhou , Lui Kathy O. , Zhou Bin
Published: April 2022

Tissue-resident macrophages play essential functions in the maintenance of tissue homeostasis and repair. Recently, the endocardium has been reported as a de novo hemogenic site for the contribution of hematopoietic cells, including cardiac macrophages, during embryogenesis. These observations challenge the current consensus that hematopoiesis originates from the hemogenic endothelium within the yolk sac and dorsal aorta. Whether the developing endocardium has such a hemogenic potential requires further investigation. Here, we generated new genetic tools to trace endocardial cells and reassessed their potential contribution to hematopoietic cells in the developing heart. Fate-mapping analyses revealed that the endocardium contributed minimally to cardiac macrophages and circulating blood cells. Instead, cardiac macrophages were mainly derived from the endothelium during primitive/transient definitive (yolk sac) and definitive (dorsal aorta) hematopoiesis. Our findings refute the concept of endocardial hematopoiesis, suggesting that the developing endocardium gives rise minimally to hematopoietic cells, including cardiac macrophages.
Acoustic wonders of Crane Mountain reproduced in backyard
Authors: Tang Mingzhong , Yang Jingyou , Hua Shuoyan , Wang Sihui , Xiao Lintao
Published: April 2022

The chirping echo after a handclap in front of the stairs found in Crane Mountain and elsewhere in the world are caused by periodic structures of the stairs acting as acoustic gratings. The sound notes of specific frequencies are enhanced due to interference effect. We reproduced the fantastic acoustic phenomena found in Crane Mountain (‘chirping sound of echoing crane’ and ‘dripping on celestial staircases’) with a ‘do it yourself’ model comprising an array of wooden boards. The ‘chirping echo’ and ‘dripping’ effects are clearly audible to human ears and can be used for demonstration in schools or public performance. With this device, we also verified that the grating equation in optics can be approximately applied to predict the chirping frequencies when incident angle and diffraction angel are small.
Periodic points and shadowing for generic Lebesgue measure-preserving interval maps
Authors: Bobok Jozef , Činč Jernej , Oprocha Piotr , Troubetzkoy Serge
Published: April 2022

In this article we study dynamical behaviour of generic Lebesgue measure-preserving interval maps. We show that for each k ⩾ 1 the set of periodic points of period at least k is a Cantor set of Hausdorff dimension zero and of upper box dimension one. Moreover, we obtain analogous results also in the context of generic Lebesgue measure-preserving circle maps. Furthermore, building on the former results, we show that there is a dense collection of transitive Lebesgue measure-preserving interval maps whose periodic points have full Lebesgue measure and whose periodic points of period k have positive measure for each k ⩾ 1. Finally, we show that the generic continuous maps of the interval which preserve the Lebesgue measure satisfy the shadowing and periodic shadowing property.
Chemical Evolution During the Formation of Molecular Clouds
Authors: Sun Jingfei , Du Fujun
Published: April 2022

To study the chemical evolution during the formation of molecular clouds, we model three types of clouds with different density structures: collapsing spherical, collapsing ellipsoidal, and static spherical profiles. The collapsing models are better than the static models in matching the observational characteristics in typical molecular clouds. This is mainly because the gravity can speed up the formation of some important molecules (e.g., H2, CO, OH) by increasing the number density during collapse. The different morphologies of prolate, oblate, and spherical clouds lead to differences in chemical evolution, which are mainly due to their different evolution of number density. We also study the effect of initial chemical compositions on chemical evolution, and find that H atoms can accelerate OH formation by two major reactions: O + H → OH in gas phase and on dust grain surfaces, leading to the models in which hydrogen is mainly atomic initially better match observations than the models in which hydrogen is mainly molecular initially. Namely, to match observations, initially hydrogen must be mostly atomic. The CO molecules are able to form even without the pre-existence of H2. We also study the influence of gas temperature, dust temperature, intensity of interstellar radiation field and cosmic-ray ionization rate on chemical evolution in static clouds. The static CO clouds with high dust temperature, strong radiation field, and intensive cosmic rays are transient due to rapid CO destruction.
A novel AIEE active anti-B18H22 derivative-based Cu2+ and Fe3+ fluorescence off-on-off sensor
Authors: Xiong Linli , Zheng Yong , Wang Haibo , Yan Jiangyang , Huang Xuguang , Meng Hongyun , Tan Chunhua
Published: April 2022

A novel fluorescence sensor for successive detection of Cu2+ and Fe3+ based on anti-B18H22 derivative which possesses 5-hydroxyisoquinoline as an ionophore was synthesized via a one-pot and its structure and photophysical properties were characterized by NMR, HRMS, FTIR, UV–vis, PL and theoretical calculation. The fluorophore displays two emission peaks at 460 nm and 670 nm in THF solution coming from the emission of the locally excited state and intramolecular charge transfer fluorescence, respectively. The complex exhibited obvious aggregation-induced emission enhancement (AIEE) characteristics in THF/H2O solution by increasing the aqueous concentration from 70% to 95%. The AIEE molecules showed a high selectivity towards Cu2+ over other metal ions by forming a 2:1 metal-to-ligand complex in THF/H2O (fw = 20%) solution, the fluorescence intensity increased as a linear function of the Cu2+ concentration at 460 nm due to the inhibition of PET effect. The fluorescent emission was quenched linearly by the addition of Fe3+, which provides a method for successive determination of Cu2+ and Fe3+ based on ‘off-on-off’ fluorescence of the fluorescent. The detection limit of Cu2+ and Fe3+ was 5.7 × 10−6 M and 7.2 × 10−5 M respectively. Morever, a rapid identification of Cu2+ in the aqueous solution by naked eyes can be realized. In addition, the molecules were pH-sensitive, the fluorescence quenching can be observed in strongly alkaline environment. The method has been applied to the determination of copper ions in water samples with satisfactory results.
Green synthesis of sorbus pohuashanensis/aronia melanocarpa extracts functionalized ZnO nanoclusters and their applications
Authors: Huang Jin , Shao Kai , Sun Jixiang , Lin Yamei , Mu Liqiang , Fu Yujie , Liu Zhiguo
Published: April 2022

ZnO nanoclusters (ZnO NCs) had been widely utilized in optoelectronics, sensors, dye removal, and antibacterial fields. To reduce or avoid the use of toxic, harmful, and costly chemical reagents, the Sorbus pohuashanensis and Aronia melanocarpa extracts were used to green synthesize ZnO NCs with superior adsorption ability for the organic dyes. The obtained ZnO NCs were characterized by UV–vis spectroscopy (UV–vis), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), x-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). TEM and SEM results indicated that the ZnO NCs tended to aggregate into large branching and sheet structures. EDS measurement confirmed the presence of zinc ions on the ZnO NCs. FTIR results revealed that the components of the fruits extracts were bounded on the surface of ZnO NCs. The primary application experiments demonstrated that the Sorbus pohuashanensis and Aronia melanocarpa extracts functionalized ZnO NCs possess effectively removing activity for organic dyes.
Microstructure evolution, texture development, and mechanical properties of hot-rolled 5052 aluminum alloy followed by annealing
Authors: Wu Jianxin , Djavanroodi Faramarz , Gode Ceren , Ebrahimi Mahmoud , Attarilar Shokouh
Published: April 2022

Aluminum alloys, especially the 5000 series, have drawn the attention of the transportation industry due to their lightweight and consequently reduced fuel consumption. In this regard, one of the major problems of this alloy is its low strength and ductility that can be solved using rolling and post-annealing. Accordingly, the present study concentrates on this issue. Microstructural images showed that the rolling process develops a lot of tangled and trapped dislocations in the sample, which gradually lead to the formation of dislocation bundles and networks. Subsequent annealing can produce a more homogeneous structure with clear grain boundaries and low dislocation density in the inner region of the grains. However, grain refinement efficiency through rolling is retained even after annealing. Initial and rolled Al5052 with the maximum intensity of 2.87 and 6.33 possess the lowest and highest overall texture. Also, post-annealing decreases the texture intensity to 6.33 and 4.87 at 150 and 200 °C, respectively. In this context, deformation texture components strengthen considerably after the rolling process due to the formation of shear bands, and they slightly weaken during heat treatment. Although the initial annealing of the as-received material does not cause discontinuous recrystallization during rolling, it may facilitate the material recovery before rolling. Post-annealing was found to decrease the improved effect of strength by rolling and increase the negative influence of ductility due to the inhibition of dislocation strengthening. The results showed that both dislocation density and the precipitation of Mg atoms are influential for electrical resistivity.
Designing silicon carbide heterostructures for quantum information science: challenges and opportunities
Authors: Harmon K J , Delegan N , Highland M J , He H , Zapol P , Heremans F J , Hruszkewycz S O
Published: April 2022

Silicon carbide (SiC) can be synthesized in a number of different structural forms known as polytypes with a vast array of optically active point defects of interest for quantum information sciences. The ability to control and vary the polytypes during SiC synthesis may offer a powerful methodology for the formation of new material architectures that expand our ability to manipulate these defects, including extending coherence lifetimes and enhancing room temperature operation. Polytypic control during synthesis presents a significant challenge given the extreme conditions under which SiC is typically grown and the number of factors that can influence polytype selection. In situ monitoring of the synthesis process could significantly expand our ability to formulate novel polytype structures. In this perspective, we outline the state of the art and ongoing challenges for precision synthesis in SiC. We discuss available in situ x-ray characterization methods that will be instrumental in understanding the atomic scale growth of SiC and defect formation mechanisms. We highlight optimistic use cases for SiC heterostructures that will become possible with in situ polytypic control and end by discussing extended opportunities for integration of ultrahigh quality SiC materials with other semiconductor and quantum materials.
Contrasting patterns of genetic diversity and differentiation across the continental disjunct range of a sexually polymorphic aquatic plant
Authors: Yakimowski Sarah B , Southcott Laura , Barrett Spencer C H
Published: April 2022

Background and Aims

Reproductive systems enabling opportunities for self-fertilization influence population genetic structure and play a key role in colonization and genetic differentiation during range expansion. Because of their well-developed powers of dispersal, aquatic plants often have widespread disjunct geographical distributions, providing opportunities to investigate the role of reproductive systems in structuring genetic variation between parts of the range that differ in migration history and ecology.

Methods

We compared reproductive systems and spatial genetic structure of the freshwater aquatic macrophyte Sagittaria latifolia between disjunct western and eastern ranges of North America (NA). Populations of this species are most commonly either monoecious or dioecious. We examined chloroplast DNA haplotype variation to test the hypothesis that the western range of this species represents a secondary colonization from the east, and evaluated the roles of reproductive system differences and geography in structuring contemporary patterns of genetic variation at 11 polymorphic SSR (simple sequence repeat) loci.

Key Results

Chloroplast haplotyping revealed a single haplotype in western NA compared to numerous haplotypes in eastern NA, consistent with a genetic bottleneck during westward migration. Estimates of genetic diversity in eastern NA populations differed significantly between reproductive systems, but this pattern was not evident in the western range. Eastern populations could be reliably assigned to genetic clusters based on their reproductive systems, whereas western populations clustered primarily by geographical location.

Conclusions

The sparser distribution of aquatic habitats in the drier western range of S. latifolia, combined with secondary colonization of this region, probably cause the lower genetic diversity and increased differentiation among populations, possibly overriding the effects of reproductive system evident in the eastern portion of the range. Our findings demonstrate that the complex interplay between migratory history, reproductive systems and habitat availability plays an important role in structuring spatial patterns of genetic variation in disjunct plant populations.
The diversification of the lynx lineage during the Plio-Pleistocene—evidence from a new smallLynxfrom Longdan, Gansu Province, China
Authors: Jianzuo Qigao , Li Lu , Madurell-Malapeira Joan , Wang Shiqi , Li Shijie , Fu Jiao , Chen Shanqin
Published: April 2022

A new small-sized lynx from Longdan, Gansu Province, China, Lynx hei sp. nov., is described in this study. The new species displays the characteristic Lynx generic traits, such as distinct buccal grooves in the upper canine, presence of an anterior groove in the upper canine, absence of upper premolar 2, and a moderately developed mastoid process, but it is markedly smaller than the previously described Lynx issiodorensis specimens from the same site and is also smaller overall than most living species, comparable to Lynx rufus in size. The new species has a relatively wide and deep zygomatic arch, similar to that of living Lynx lynx, Lynx pardinus and Lynx canadensis but wider than that of Lynx rufus. Our phylogenetic analyses suggest that Lynx hei falls within the crown group Lynx, being the sister to Lynx rufus or, less probably, a sister to Lynx issiodorensis + three other living species of Lynx. The Plio-Pleistocene Lynx issiodorensis is supported as the ancestor of Lynx lynx, Lynx pardinus and Lynx canadensis. Our phylogenetic study suggests that Lynx diversification over the Plio-Pleistocene was achieved initially by body size differentiation, putatively forced by intraspecific competition with other carnivorans, followed by morphological divergence.
From cardiorenal syndromes to cardionephrology: a reflection by nephrologists on renocardiac syndromes
Authors: Quiroga Borja , Ortiz Alberto , Navarro-González Juan F , Santamaría Rafael , de Sequera Patricia , Díez Javier
Published: April 2022

Cardiorenal syndromes (CRS) are broadly defined as disorders of the heart and kidneys whereby acute or chronic dysfunction in one organ may induce acute or chronic dysfunction of the other. CRS are currently classified into five categories, mostly based on disease-initiating events and their acuity or chronicity. CRS types 3 and 4 (also called renocardiac syndromes) refer to acute and chronic kidney dysfunction resulting in acute and chronic heart dysfunction, respectively. The notion of renocardiac syndromes has broadened interest in kidney–heart interactions but uncertainty remains in the nephrological community's understanding of the clinical diversity, pathophysiological mechanisms and optimal management approaches of these syndromes. This triple challenge that renocardiac syndromes (and likely other cardiorenal syndromes) pose to the nephrologist can only be faced through a specific and demanding training plan to enhance his/her cardiological scientific knowledge and through an appropriate clinical environment to develop his/her cardiological clinical skills. The first must be the objective of the subspecialty of cardionephrology (or nephrocardiology) and the second must be the result of collaboration with cardiologists (and other specialists) in cardiorenal care units. This review will first consider various aspects of the challenges that renocardiac syndromes pose to nephrologists and, then, will discuss those aspects of cardionephrology and cardiorenal units that can facilitate an effective response to the challenges.
Serum bilirubin and kidney function: a Mendelian randomization study
Authors: Park Sehoon , Lee Soojin , Kim Yaerim , Lee Yeonhee , Kang Min Woo , Kim Kwangsoo , Kim Yong Chul , Han Seung Seok , Lee Hajeong , Lee Jung Pyo , Joo Kwon Wook , Lim Chun Soo , Kim Yon Su , Kim Dong Ki
Published: April 2022

Background

Further investigation is needed to determine the causal effects of serum bilirubin on the risk of chronic kidney disease (CKD).

Methods

This study is a Mendelian randomization (MR) analysis. Among the well-known single-nucleotide polymorphisms (SNPs) related to serum bilirubin levels, rs4149056 in the SLCO1B1 gene was selected as the genetic instrument for single-variant MR analysis, as it was found to be less related to possible confounders than other SNPs. The association between genetic predisposition for bilirubin levels and estimated glomerular filtration rate (eGFR) or CKD was assessed in 337 129 individuals of white British ancestry from the UK Biobank cohort. Two-sample MR based on summary-level data was also performed. SNPs related to total or direct bilirubin levels were collected from a previous genome-wide association study and confounder-associated SNPs were discarded. The independent CKDGen meta-analysis data for CKD were employed as the outcome summary statistics.

Results

The alleles of rs4149056 associated with higher bilirubin levels were associated with better kidney function in the UK Biobank data. In the summary-level MR, both of the genetically predicted total bilirubin {per 5 µmol/L increase; odds ratio [OR] 0.931 [95% confidence interval (CI) 0.871–0.995]} and direct bilirubin [per 1 µmol/L increase; OR 0.910 (95% CI 0.834–0.993)] levels were significantly associated with a lower risk of CKD, supported by the causal estimates from various MR sensitivity analyses.

Conclusion

Genetic predisposition for higher serum bilirubin levels is associated with better kidney function. This result suggests that higher serum bilirubin levels may have causal protective effects against kidney function impairment.
Capital Theory and Effective Demand
Authors: Bhaduri Amit
Published: April 2022

Using simple national income accounting and a fixed coefficient two sector model this paper examines how the problem of capital valuation upsets the foundations of the marginal productivity theory of distribution. Assuming no capital valuation problem and zero Wicksell effect, both marginalist theory and the labour theory of value hold. However, the same equation that makes the Wicksell effect zero also entails the profit realization condition of effective demand for the ‘golden age’. This argument is extended providing a new perspective to the link between effective demand and capital theory.
Perforated cuspal aneurysm of aortic valve following infective endocarditis presenting as complete heart block: a case report and review of literature
Authors: Kunal Shekhar , Shah Bhushan , Bagarhatta Rajeev , Verma Hemlata
Published: April 2022

Aortic cuspal aneurysm is a rare clinical entity and often occurs as a complication of infective endocarditis. We report a case of a 30-year-old male with no prior comorbid conditions who presented with fever, acute onset shortness of breath, and chest pain along with multiple episodes of syncope. Electrocardiogram revealed complete heart block while two-dimensional echocardiogram was suggestive of perforated aortic cuspal aneurysm with aortic regurgitation. Blood cultures were positive for Streptococcus viridans. The patient was initiated on broad spectrum antibiotics, temporary pacemaker implantation, and subsequently underwent aortic valve replacement followed by permanent pacemaker implantation after 6 weeks. A diagnosis of perforated aortic cuspal aneurysm subsequent to infective endocarditis was made. This was based on clinical presentation, echocardiographic evaluation, blood cultures, and surgical as well as histopathological findings.
Message in a Bottle—Metabarcoding enables biodiversity comparisons across ecoregions
Authors: Steinke D , deWaard S L , Sones J E , Ivanova N V , Prosser S W J , Perez K , Braukmann T W A , Milton M , Zakharov E V , deWaard J R , Ratnasingham S , Hebert P D N
Published: April 2022

Background

Traditional biomonitoring approaches have delivered a basic understanding of biodiversity, but they cannot support the large-scale assessments required to manage and protect entire ecosystems. This study used DNA metabarcoding to assess spatial and temporal variation in species richness and diversity in arthropod communities from 52 protected areas spanning 3 Canadian ecoregions.

Results

This study revealed the presence of 26,263 arthropod species in the 3 ecoregions and indicated that at least another 3,000–5,000 await detection. Results further demonstrate that communities are more similar within than between ecoregions, even after controlling for geographical distance. Overall α-diversity declined from east to west, reflecting a gradient in habitat disturbance. Shifts in species composition were high at every site, with turnover greater than nestedness, suggesting the presence of many transient species.

Conclusions

Differences in species composition among their arthropod communities confirm that ecoregions are a useful synoptic for biogeographic patterns and for structuring conservation efforts. The present results also demonstrate that metabarcoding enables large-scale monitoring of shifts in species composition, making it possible to move beyond the biomass measurements that have been the key metric used in prior efforts to track change in arthropod communities.
Near-real-time detection of co-seismic ionospheric disturbances using machine learning
Authors: Brissaud Quentin , Astafyeva Elvira
Published: April 2022

Tsunamis generated by large earthquake-induced displacements of the ocean floor can lead to tragic consequences for coastal communities. Measurements of co-seismic ionospheric disturbances (CIDs) offer a unique solution to characterize an earthquake’s tsunami potential in near-real-time (NRT) since CIDs can be detected within 15 min of a seismic event. However, the detection of CIDs relies on human experts, which currently prevents the deployment of ionospheric methods in NRT. To address this critical lack of automatic procedure, we designed a machine-learning-based framework to (1) classify ionospheric waveforms into CIDs and noise, (2) pick CID arrival times and (3) associate arrivals across a satellite network in NRT. Machine-learning models (random forests) trained over an extensive ionospheric waveform data set show excellent classification and arrival-time picking performances compared to existing detection procedures, which paves the way for the NRT imaging of surface displacements from the ionosphere.
Stigma Dynamics: Russia and the Crisis of Liberal Ordering
Authors: Rogstad Adrian
Published: April 2022

The concepts of stigma and stigmatization have recently made inroads into IR. However, scholarship on stigmatization has primarily focused on discrete instances of stigmatization and stigma management for the purposes of comparison. Less has been written about how the dynamics of stigmatization processes can alter over time as actors change stigma-management tactics and stigmatization varies in intensity, and the effect this may have on social norms. To that end, this article introduces a distinction between direct and diffuse forms of stigmatization that, when combined with different forms of stigma management, lead to changes in stigma dynamics over time. It applies this distinction to Russian stigma management vis-à-vis liberal international order, specifically the norms of democracy and human rights that constitute a “normal” for states to aspire to, any deviance from which requires stigma management. By outlining the interactive process of Russia's stigma management in relation to these norms since the end of the Cold War, and the degrees of direct and diffuse stigmatization of Russia by the norms’ promoters, the article also seeks to make a contribution to work on the crisis of liberal international order, aligning itself with contributions focused on the co-constitution of order through processes of ordering. The article proposes that stigmatization is a constitutive mechanism of liberal ordering and that some of the explanation for the supposed crisis of liberal order is the alteration of international stigma dynamics.
Structural and biochemical analyses of the nucleosome containing Komagataella pastoris histones
Authors: Fukushima Yutaro , Hatazawa Suguru , Hirai Seiya , Kujirai Tomoya , Ehara Haruhiko , Sekine Shun-ichi , Takizawa Yoshimasa , Kurumizaka Hitoshi
Published: April 2022

Komagataella pastoris is a methylotrophic yeast that is commonly used as a host cell for protein production. In the present study, we reconstituted the nucleosome with K. pastoris histones and determined the structure of the nucleosome core particle by cryogenic electron microscopy. In the K. pastoris nucleosome, the histones form an octamer and the DNA is left-handedly wrapped around it. Micrococcal nuclease assays revealed that the DNA ends of the K. pastoris nucleosome are somewhat more accessible, as compared with those of the human nucleosome. In vitro transcription assays demonstrated that the K. pastoris nucleosome is transcribed by the K. pastoris RNA polymerase II (RNAPII) more efficiently than the human nucleosome, while the RNAPII pausing positions of the K. pastoris nucleosome are the same as those of the human nucleosome. These results suggested that the DNA end flexibility may enhance the transcription efficiency in the nucleosome but minimally affect the nucleosomal pausing positions of RNAPII.
Salvage of an iatrogenic partial allograft nephrectomy during caesarean section—a case report
Authors: Shaheen Mohammed F , Alsugair Sulaiman , AbuMelha Saad M , Almarastani Mohamad , Altheaby Abdulrahman
Published: April 2022

Since transplanted kidney allografts are standardly placed in a heterotopic pelvic position, any surgical intervention in the pelvis carries the potential risk for allograft injury. A 41-year-old female G4P2 with history of prior kidney transplants presented for an elective cesarean sections (CS). During the CS, profound bleeding was encountered and was later realized to be from the transplanted kidney allograft. A complete transection of the upper pole with the injury extending to the hilar structures was noted. Careful intraoperative evaluation led to the decision favoring a salvage attempt of the remaining part of the allograft. The patient continues to enjoy sufficient allograft function 6 months after the incident. To conclude, although CS after kidney transplantation is considered safe, the risk of allograft injury remains possible with potentially catastrophic consequences. This case highlights the importance of rapid surgical expertise and appropriate preoperative evaluation, preparation and planning.
Deciphering the Lyman-α emission line: towards the understanding of galactic properties extracted from Lyα spectra via radiative transfer modelling
Authors: Li Zhihui , Gronke Max
Published: April 2022

Existing ubiquitously in the Universe with the highest luminosity, the Lyman-α (Lyα) emission line encodes abundant physical information about the gaseous medium it interacts with. Nevertheless, the resonant nature of the Lyα line complicates the radiative transfer (RT) modelling of the line profile. We revisit the problem of deciphering the Lyα emission line with RT modelling. We reveal intrinsic parameter degeneracies in the widely used shell model in the optically thick regime for both static and outflowing cases, which suggest the limitations of the model. We also explore the connection between the more physically realistic multiphase, clumpy model, and the shell model. We find that the parameters of a ‘very clumpy’ slab model and the shell model have the following correspondences: (1) the total column density, the effective temperature, and the average radial clump outflow velocity of the clumpy slab model are equal to the H i column density, effective temperature, and expansion velocity of the shell model, respectively; (2) large intrinsic linewidths are required in the shell model to reproduce the wings of the clumpy slab models; (3) adding another phase of hot interclump medium increases peak separation, and the fitted shell expansion velocity lies between the outflow velocities of two phases of gas. Our results provide a viable solution to the major discrepancies associated with Lyα fitting reported in previous literature, and emphasize the importance of utilizing information from additional observations to break the intrinsic degeneracies and interpreting the model parameters in a more physically realistic context.
sRNAbench and sRNAtoolbox 2022 update: accurate miRNA and sncRNA profiling for model and non-model organisms
Authors: Aparicio-Puerta Ernesto , Gómez-Martín Cristina , Giannoukakos Stavros , Medina José María , Scheepbouwer Chantal , García-Moreno Adrián , Carmona-Saez Pedro , Fromm Bastian , Pegtel Michiel , Keller Andreas , Marchal Juan Antonio , Hackenberg Michael
Published: April 2022

The NCBI Sequence Read Archive currently hosts microRNA sequencing data for over 800 different species, evidencing the existence of a broad taxonomic distribution in the field of small RNA research. Simultaneously, the number of samples per miRNA-seq study continues to increase resulting in a vast amount of data that requires accurate, fast and user-friendly analysis methods. Since the previous release of sRNAtoolbox in 2019, 55 000 sRNAbench jobs have been submitted which has motivated many improvements in its usability and the scope of the underlying annotation database. With this update, users can upload an unlimited number of samples or import them from Google Drive, Dropbox or URLs. Micro- and small RNA profiling can now be carried out using high-confidence Metazoan and plant specific databases, MirGeneDB and PmiREN respectively, together with genome assemblies and libraries from 441 Ensembl species. The new results page includes straightforward sample annotation to allow downstream differential expression analysis with sRNAde. Unassigned reads can also be explored by means of a new tool that performs mapping to microbial references, which can reveal contamination events or biologically meaningful findings as we describe in the example. sRNAtoolbox is available at: https://arn.ugr.es/srnatoolbox/.
High Rates of Asymptomatic Mycoplasma genitalium Infections With High Proportion of Genotypic Resistance to First-Line Macrolide Treatment Among Men Who Have Sex With Men Enrolled in the Zurich Primary HIV Infection Study
Authors: Ring Alexander , Balakrishna Suraj , Imkamp Frank , Burkard Sara , Triet Flurina , Brunschweiler Flurina , Grube Christina , Bodmer Rebecca , Kouyos Roger D , Günthard Huldrych F , Braun Dominique L
Published: April 2022

Background

Mycoplasma genitalium (Mg) is an emerging sexually transmitted pathogen among men who have sex with men (MSM). Resistance to recommended antimicrobial agents are of public health concern. Few data exist on Mg infections in MSM diagnosed with human immunodeficiency virus (HIV) during primary HIV infection.

Methods

Participants of the Zurich Primary HIV Study (ClinicalTrials.gov Identifier NCT 00537966) were systematically offered screening for sexually transmitted infections (STIs) between April 2019 and September 2020. Screening was performed using an in-house polymerase chain reaction panel comprising Mg including genotypic resistance testing for macrolides and quinolones, Chlamydia trachomatis including serovars L1-L3, Neisseria gonorrhoeae, Treponema pallidum, and Hemophilus ducreyi.

Results

We screened 148 of 266 (55.6%) participants, with an overall total of 415 follow-up visits. Ninety-one percent were MSM. The incidence rate for all STIs was 47.0 (95% confidence interval [CI], 32.2–68.6) per 100 person-years. Mycoplasma genitalium was the most frequently detected pathogen: 30 participants (20%) presented with at least 1 Mg infection, corresponding to a period prevalence of 20.3% and incidence rate of 19.5 Mg infections (95% CI, 11.8–32.4). Most Mg infections (93%) were asymptomatic, and 9 (30%) participants showed spontaneous clearance. We detected high rates of antibiotic resistance: 73.3% to macrolides, 3.3% to quinolones, and 13.3% resistance to both antibiotics.

Conclusions

The high prevalence of mostly asymptomatic Mg infections and high rate of spontaneous clearance support cautious initiation for treatment. The high proportion of macrolide-resistant strains suggests that a genotypic determination of resistance should be standard of care. Moxifloxacin should be the preferred treatment option for symptomatic Mg infections among MSM if resistance testing is unavailable.
The Role of Isavuconazonium Sulphate for the Treatment of Blastomycosis: A Case Series and Antifungal Susceptibility
Authors: Scolarici Michael J , King Coleton , Sterkel Alana , Smith Jeannina , Gauthier Gregory , Saddler Christopher
Published: April 2022

Background

Blastomyces spp, the etiologic agents of blastomycosis, are endemic dimorphic fungi that require prolonged antifungal therapy, which can be complicated by adverse drug effects. Isavuconazonium sulphate (ISA) is a triazole with in vitro and in vivo activity against Blastomyces spp, but there is a paucity of clinical data supporting its use for treatment of blastomycosis.

Methods

This retrospective case series identified 14 patients with blastomycosis at least partially treated with ISA at the University of Wisconsin between 2015 and 2019. Treatment duration and outcomes were documented. In addition, 29 clinical isolates of Blastomyces spp between 2004 and 2017 were tested for minimum inhibitory concentrations against ISA and other antifungals.

Results

Fourteen patients were treated with a median of 255 days of ISA accounting for 68% of total therapy. Half (7 of 14) of the patients were immunocompromised, 11 of 14 (79%) were proven cases of blastomycosis, 7 of 14 (50%) had central nervous system (CNS) involvement, and 11 of 14 (79%) were cured. Antifungal susceptibility testing showed a consistently low minimum inhibitory concentration to ISA ≤ 0.015 mcg/mL.

Conclusions

This case series supports the efficacy and safety for ISA in the treatment of blastomycosis with or without CNS disseminated, especially when alternative triazoles cannot be used.
Inference of magnetic field during the Dalton minimum: Case study with recorded sunspot areas
Authors: Uneme Shoma , Imada Shinsuke , Lee Harim , Park Eunsu , Hayakawa Hisashi , Iju Tomoya , Moon Yong-Jae
Published: April 2022

The Dalton minimum is considered to be one of the unique solar activity periods that have been captured in direct sunspot observations since 1610. Specifically, the solar magnetic field in this period is of great interest. Derfflinger and Prantner’s sunspot observations of 1802–1824 and 1800–1844 are the most important references for this period. To understand the solar magnetic activity in the Dalton minimum, it is important to estimate the latitude/longitude distribution of the sunspots and the sunspot areas for that duration. In this study, we analyze Derfflinger and Prantner’s sunspot drawings to determine the sunspot parameters, particularly the sunspot area. We find that the sunspot areas obtained from Derfflinger’s drawings are overemphasized by a factor of eight relative to those derived from modern observations. We also analyze Prantner’s sunspot drawings to validate our analysis of Derfflinger’s drawings. Further, we generate solar magnetograms from Derfflinger’s sunspot drawings using a deep-learning model based on conditional generative adversarial networks. Our analysis of these sunspot areas will provide important information for restoring the magnetograms during the Dalton minimum.
All hands on deck: A multidisciplinary approach to SARS-CoV-2-associated MIS-C
Authors: Lopez Alison A , Patel Mona , Rayment Jonathan H , Tam Herman , Roberts Ashley , Laskin Samara , Tucker Lori , Biggs Catherine M ,   BMWG, Ahmed May A , Barakauskas Vilte , Galanis Eleni , Goldfarb David , Halparin Jessica , Harris Kevin C , Kissoon Niranjan , Lawrence Sally , McGuffin Dominique , Meckler Garth , Noel Kirsten , Schrader Dewi , Sherwood Michelle , Singh Avash , Vercauteren Suzanne
Published: April 2022

Background

Multisystem Inflammatory Syndrome in Children (MIS-C) is a post-infectious complication of SARS-CoV-2 infection with overlapping features of Kawasaki disease and toxic shock syndrome. In May 2020, a provincial multidisciplinary working group was established in anticipation of emerging cases following the first wave of SARS-CoV-2 infections.

Methodology

Our centre established a multidisciplinary working group for MIS-C cases in British Columbia. The group developed guidelines using the World Health Organization MIS-C case definition. Guidelines were updated using quality improvement methods as new reports and our local experience evolved. We included all children who were evaluated in person or had samples sent to our centre for MIS-C evaluation from May 2020 to April 2021. We prospectively collected patient demographics, clinical and laboratory characteristics, and treatment.

Results

Fifty-two children were included. Eleven were diagnosed as confirmed MIS-C. Ten of the 11 MIS-C cases presented with shock. Gastrointestinal and mucocutaneous involvement were also prominent. Common laboratory features included elevated C-reactive protein, D-dimer, troponin, and brain natriuretic peptide. Four out of 11 (36%) had myocardial dysfunction and 3/11 (27%) had coronary artery abnormalities. All 11 patients had evidence of SARS-CoV-2 infection. Ten out of 11 (91%) received intravenous (IV) immunoglobulin and IV corticosteroids.

Conclusion

Our provincial cohort of MIS-C patients were more likely to present with shock and cardiac dysfunction, require ICU admission, and be treated with corticosteroids compared to ruled out cases. Our working group’s evolving process ensured children with features of MIS-C were rapidly identified, had standardized evaluation, and received appropriate treatment in our province.
Anatomical Topographical Investigation of the Medial Branch of the Dorsal Thoracic Branch of the Spinal Nerve in the Segments T10—T12
Authors: Koutp Amir , Petritsch Johanna , Skias Christoph , Grechenig Peter , Andrianakis Alexandros , Kieser David C , Feigl Georg C
Published: April 2022

Introduction

The zygapophyseal joints represent one possible cause for back pain. Therefore, many interventions are targeting the denervation of the facet joints. The aim of this study is to describe the course of the medial branch of the dorsal branch of the spinal nerve and its articular branches to the zygapophyseal joints in the segments T10–T12.

Methods

The medial branches in the thoracic segments T10–T12 were dissected in 20 Thiel embalmed cadavers. An Eschenbach magnifying glass (4.0× magnification) was used during dissection preserving the articular branches. The topography and the branching pattern of the medial branches was observed.

Results

The course of the nerves in the segments T10–T12 differed from each other because of the different osseous anatomy of each segment. The medial branch at the segment T10 crossed the tip of the transverse process in 28 of the 40 hemivertebral specimens. In the remaining cases it passed superior to the transverse process. At T11 the medial branch ran constantly through an osteofibrous canal. At the segment T12 the medial branches showed a similar course to the medial branches in the lumbar region. In many cases two articular branches, which arose from the medial branch were identified.

Conclusions

The results of this study show a considerable anatomic variety at the segment T10. It also demonstrates that the transverse process is an important landmark to encounter the medial branch. Furthermore, the possibility of a double innervation of the facet joints should always be considered.
Third dose vaccination with mRNA-1273 or BNT162b2 vaccines improves protection against SARS-CoV-2 infection
Authors: Niesen Michiel J M , Matson Robert , Puranik Arjun , O'Horo John C , Pawlowski Colin , Vachon Celine , Challener Douglas , Virk Abinash , Swift Melanie , Speicher Leigh , Gordon Joel , Geyer Holly , Lenehan Patrick J , Venkatakrishnan A J , Soundararajan Venky , Badley Andrew
Published: April 2022

As of 2021 November 29, booster vaccination against SARS-CoV-2 infection has been recommended for all individuals aged 18 years and older in the United States. A key reason for this recommendation is the expectation that a booster vaccine dose can alleviate observed waning of vaccine effectiveness (VE). Although initial reports of booster effectiveness have been positive, the level of protection from booster vaccination is unclear. We conducted two studies to assess the impact of booster vaccination, with BNT162b2 or mRNA-1273, on the incidence of SARS-CoV-2 infection between August and December 2021. We first compared SARS-CoV-2 infection incidence in cohorts of 3-dose vaccine recipients to incidence in matched cohorts of 2-dose vaccine recipients (cohort size = 24,539 for BNT162b2 and 14,004 for mRNA-1273). Additionally, we applied a test-negative study design to compare the level of protection against symptomatic infection in 3-dose recipients to that observed in recent 2-dose primary vaccine series recipients. The 3-dose recipients experienced a significantly lower incidence rate of SARS-CoV-2 infection than the matched 2-dose cohorts (BNT162b2 Incidence Rate Ratio: 0.11, 95% CI: 0.09 to 0.13 and mRNA-1273 IRR: 0.11, 95% CI: 0.08 to 0.15). Results from the test-negative study showed the third vaccine dose mitigated waning of VE, with the risk of symptomatic infection in 3-dose recipients being comparable to that observed 7 to 73 days after the primary vaccine series. These results show that 3-dose vaccine regimens with BNT162b2 or mRNA-1273 are effective at reducing SARS-CoV-2 infection and support the widespread administration of booster vaccine doses.
Periostin Attenuates Cyclophosphamide-induced Bladder Injury by Promoting Urothelial Stem Cell Proliferation and Macrophage Polarization
Authors: Chen Zhihong , Liu Liyong , Chen Yunhua , Liu Minjie , Peng Xiang Andy , Deng Chunhua , Jiang Mei Hua
Published: April 2022

Interstitial cystitis (IC) is a bladder syndrome of unclear etiology with no generally accepted treatment. Growing evidence suggest that periostin (POSTN) is an important homeostatic component in the tissue repair and regeneration in adulthood, but its function in urinary bladder regeneration is still unknown. Here we investigate whether POSTN is involved in bladder tissue repair in a cyclophosphamide (CYP)-induced interstitial cystitis model. POSTN is primarily expressed in bladder stroma (detrusor smooth muscle and lamina propria) and upregulated in response to CYP-induced injury. POSTN deficiency resulted in more severe hematuria, aggravated edema of the bladder, and delayed umbrella cell recovery. Besides, less proliferative urothelial cells (labeled by pHH3, Ki67, and EdU) and lower expression of Krt14 (a urothelial stem cell marker) were detected in POSTN−/− mice post CYP exposure, indicating a limited urothelial regeneration. Further investigations revealed that POSTN could induce Wnt4 upregulation and activate AKT signaling, which together activates β-catenin signaling to drive urothelial stem cell proliferation. In addition, POSTN can promote resident macrophage proliferation and polarization to a pro-regenerative (M2) phenotype, which favors urothelial regeneration. Furthermore, we generated injectable P-GelMA granular hydrogel as a biomaterial carrier to deliver recombinant POSTN into the bladder, which could increase urothelial stem cells number, decrease umbrella cells exfoliation, and hence alleviate hematuria in a CYP-induced interstitial cystitis model. In summary, our findings identify a pivotal role of POSTN in bladder urothelial regeneration and suggest that intravesical biomaterials-assisted POSTN delivery may be an efficacious treatment for interstitial cystitis.
Highly efficient libraries design for saturation mutagenesis
Authors: Pines Gur , Pines Assaf , Eckert Carrie A
Published: April 2022

Saturation mutagenesis is a semi-rational approach for protein engineering where sites are saturated either entirely or partially to include amino acids of interest. We previously reported on a codon compression algorithm, where a set of minimal degenerate codons are selected according to user-defined parameters such as the target organism, type of saturation and usage levels. Here, we communicate an addition to our web tool that considers the distance between the wild-type codon and the library, depending on its purpose. These forms of restricted collections further reduce library size, lowering downstream screening efforts or, in turn, allowing more comprehensive saturation of multiple sites. The library design tool can be accessed via http://www.dynamcc.com/dynamcc_d/.

Graphical Abstract
Correlation between morphology parameters and skid resistance of asphalt pavement
Authors: Song Weimin
Published: April 2022

Pavement skid resistance is critical for contributing to traffic safety. In this study, two pavement specimens were fabricated using different aggregate gradations and binder contents. Compaction efforts were made to ensure the same porosity of the two specimens. Some parameters related to the surface morphology were measured using the sand patching method and a 3D laser. The parameters include mean texture depth (MTD), interface roughness (Ra), skewness (Rsk), kurtosis (Rku), fractal dimension (D) and parameters related to material ratio curve. Relationships between these parameters and skid resistance were quantitatively developed. Skid resistance increased along with the increase of MTD, Raand D. From the surface A to surface B, MTDincreased from 0.23 to 1.10 mm, Ra increased from 0.93 to 1.80 mm, Dincreased from 2.25 to 2.43 and the British Pendulum Number increased from 80 to 97 accordingly. The comprehensive analysis could assist scholars and practitioners to facilitate the understanding of tyre-pavement interaction and further assist pavement design.