bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive Evaluation of Multi-Omics Clustering Algorithms for Cancer Molecular Subtyping
Authors: Wang Juan , Wang Lingxiao , Liu Yi , Li Xiao , Ma Jie , Li Mansheng , Zhu Yunping
Published: January 2025

As a highly heterogeneous and complex disease, the identification of cancer’s molecular subtypes is crucial for accurate diagnosis and personalized treatment. The integration of multi-omics data enables a comprehensive interpretation of the molecular characteristics of cancer at various biological levels. In recent years, an increasing number of multi-omics clustering algorithms for cancer molecular subtyping have been proposed. However, the absence of a definitive gold standard makes it challenging to evaluate and compare these methods effectively. In this study, we developed a general framework for the comprehensive evaluation of multi-omics clustering algorithms and introduced an innovative metric, the accuracy-weighted average index, which simultaneously considers both clustering performance and clinical relevance. Using this framework, we performed a thorough evaluation and comparison of 11 state-of-the-art multi-omics clustering algorithms, including deep learning-based methods. By integrating the accuracy-weighted average index with computational efficiency, our analysis reveals that PIntMF demonstrates the best overall performance, making it a promising tool for molecular subtyping across a wide range of cancers.
Structural and Magnetic Properties of Biogenic Nanomaterials Synthesized by Desulfovibrio sp. Strain A2
Authors: Platunov Mikhail S. , Knyazev Yuriy V. , Ikkert Olga P. , Karnachuk Olga V. , Nikolenko Anton D. , Svetogorov Roman D. , Khramov Evgeny V. , Volochaev Mikhail N. , Dubrovskiy Andrey A.
Published: January 2025

This study explores the phase composition, local atomic structure, and magnetic properties of biogenic nanomaterials synthesized through microbially mediated biomineralization by the sulfate-reducing bacterium Desulfovibrio species strain A2 (Cupidesulfovibrio). Using X-ray diffraction (XRD), transmission electron microscopy (TEM), Mössbauer spectroscopy, X-ray absorption near-edge structure (XANES) spectroscopy, extended X-ray absorption fine structure (EXAFS) spectroscopy, and magnetic measurements, we identified a mixture of vivianite (Fe3(PO4)2·8H2O) and sulfur-containing crystalline phases (α-sulfur). XRD analysis confirmed that the vivianite phase, with a monoclinic I2/m structure, constitutes 44% of the sample, while sulfur-containing phases (α-sulfur, Fddd) account for 56%, likely as a result of bacterial sulfate-reducing activity. X-ray absorption spectroscopy (XAS) and EXAFS revealed the presence of multiple sulfur oxidation states, including elemental sulfur and sulfate (S6+), underscoring the role of sulfur in the sample’s structure. Mössbauer spectroscopy identified the presence of ferrihydrite nanoparticles with a blocking temperature of approximately 45 K. Magnetic measurements revealed significant coercivity (~2 kOe) at 4.2 K, attributed to the blocked ferrihydrite nanoparticles. The results provide new insights into the structural and magnetic properties of these microbially mediated biogenic nanomaterials, highlighting their potential applications in magnetic-based technologies.
Growth Rate Prediction, Performance, and Biochemical Enhancement of Black Soldier Fly (Hermetia illucens) Fed with Marine By-Products and Co-Products: A Potential Value-Added Resource for Marine Aquafeeds
Authors: Rodrigues Daniela P. , Calado Ricardo , Pinho Marisa , Domingues M. do Rosário , Vázquez José Antonio , Ameixa Olga M. C. C.
Published: January 2025

Aquafeed production is a fast-growing industry, seeking novel, cost-efficient raw materials to diversify traditional ingredients like fish meal and oil. Insects, particularly BSF larvae, convert by-products and waste into value-added biomass. In this study, by-products and co-products from two major fish-transforming industries in the Iberian Peninsula, i.e., tuna heads (THs) and codfish frames (CFs), hydrolysates of THs and CFs, and TH oils, were supplied to BSF larvae to improve their profile in n-3 fatty acids (FAs), namely EPA and DHA, and their protein/amino acid content. By testing the replacement levels of a control diet with by-products and co-products, we evaluated the amount of n-3 FA that could be added to BSF larval tissues. The results showed that high levels of a hydrolysed diet negatively impacted larval survival. In addition, parameters such as the moisture, protein content, and viscosity of the substrate affected bioconversion rates. Nevertheless, BSF fed with these diets contained high levels of lysine (5.8–8.4%, dry weight (DW)), methionine (1.5–2.4%, DW), and n-3 FA (14.4% DW: EPA 6.7% and DHA 7.1%). These findings suggest that BSF can effectively convert fish by-products into a nutrient-rich biomass for aquafeeds, supporting the diversification of raw material sources and promoting a circular bioeconomy.
Mechanisms, Biomarkers, and Treatment Approaches for Diabetic Kidney Disease: Current Insights and Future Perspectives
Authors: Joumaa Jean Paule , Raffoul Angela , Sarkis Charbel , Chatrieh Elizabeth , Zaidan Sally , Attieh Philippe , Harb Frederic , Azar Sami , Ghadieh Hilda E.
Published: January 2025

Diabetic Kidney Disease (DKD) is the leading cause of end-stage renal disease (ESRD) worldwide. Among individuals with type 1 diabetes mellitus (T1DM), 30–40% are at risk of developing DKD. This review focuses on the mechanistic processes, available and emerging biomarkers for diagnosing, monitoring, and preventing DKD, as well as treatment options targeted at DKD patients. A literature search was conducted on PubMed and Scopus using specific keywords. Inclusion and exclusion criteria were applied to select the articles used for this review. The literature highlights various mechanisms involved in the progression of DKD to more severe stages. Additionally, several biomarkers have been identified, which aid in diagnosing and monitoring the disease. Furthermore, numerous treatment approaches are being explored to address the underlying causes of DKD. Advanced research is exploring new medications to aid in DKD remission; sodium-glucose cotransport (SGLT2) inhibitors and finerenone, in particular, are gaining attention for their novel renoprotective effects. DKD is a major complication of diabetes, marked by complex and multifactorial mechanisms. Thus, understanding these processes is essential for developing targeted therapies to potentially reverse DKD progression. Biomarkers show promise for early diagnosis and monitoring of disease progression, while current treatment strategies underscore the importance of a multifaceted approach.
Acceptance of Illness and Health-Related Quality of Life in Patients After Myocardial Infarction—Narrative Review
Authors: Tokarewicz Justyna , Jankowiak Barbara , Klimaszewska Krystyna , Święczkowski Michał , Matlak Krzysztof , Dobrzycki Sławomir
Published: January 2025

Introduction: Cardiovascular diseases, particularly myocardial infarction (MI), significantly impact patients’ lives, causing stress and prompting varied responses to illness. Aim and methods: We conducted a narrative review concerning the acceptance of illness and quality of life in post-MI patients. Based on an extensive search of the available literature, this review consolidates current evidence on the proposed topic. Conclusions and implications: While some patients struggle with acceptance and face emotional distress, others who accept their condition are more likely to engage in treatment and lifestyle changes, leading to an improved health-related quality of life (HRQoL). Following an MI, patients often experience depression, anxiety, and stress, complicating their acceptance of the illness. Risk factors, such as hypertension, diabetes, and smoking, play a significant role in influencing HRQoL in post-MI patients. An accurate assessment of HRQoL is crucial for tailoring effective treatments and support strategies to enhance patient outcomes and identify those most at risk of developing post-MI depression or anxiety. Effective physician–patient and nurse–patient communication and support from family might be helpful in recovery. Cardiac rehabilitation improves patients’ outcomes and HRQoL. This review underscores the importance of integrating psychological support with optimal medical care to improve patient prognosis and enhance the HRQoL of individuals recovering from MI. The healthcare system could implement routine psychological assessments for MI patients at admission and discharge to establish a baseline for follow-up. Future research should explore effective psychological interventions, the interplay of CVD risk factors and psychosocial aspects, the emerging role of artificial intelligence in personalized care, and the cost-effectiveness of integrated treatment models.
Effects of Anticancer Therapy on Osteoporosis in Breast Cancer Patients: A Nationwide Study Using Data from the National Health Insurance Service-National Health Information Database
Authors: Kwon Minji , Kim Bo-Hyung , Min Sun Young , Chae Sumin
Published: January 2025

Background/Objectives: This nationwide retrospective study evaluated the effects of anticancer therapy on osteoporosis in 126,132 Korean breast cancer survivors from 2002 to 2020. Methods: The Cox proportional hazards model assessed the effects of treatment on osteoporosis. To circumvent the guarantee-time bias for osteoporosis development, a landmark analysis was employed. A stabilized inverse probability of treatment weighting was performed to control any confounding bias. The propensity score was calculated using a multinomial logistic regression model with age, national health insurance, and the Charlson comorbidity index. Results: During a median follow-up of 4.22 years, 28,603 cases of osteoporosis were documented. Aromatase inhibitors (AIs) were associated with a higher risk of osteoporosis development in comparison to tamoxifen (TMX) or chemotherapy. Notably, AIs administered subsequent to a combination of chemotherapy and anti-HER2 therapy exhibited the highest risk of osteoporosis development. Subgroup analysis revealed that the mean interval from breast cancer diagnosis to osteoporosis development was 5.00 years for women diagnosed with cancer at age < 50 and 3.89 years for those diagnosed at age ≥ 60. TMX increased the risk of osteoporosis in women diagnosed with cancer at age < 50, whereas chemotherapy was not a significant risk factor for osteoporosis development in those diagnosed at age ≥ 60. The impact of anticancer therapy on osteoporosis development was more pronounced in women diagnosed with breast cancer at a younger age compared to those diagnosed at an older age. Conclusions: Effective prevention and active management strategies should be implemented to address bone loss in both younger and older breast cancer patients.
Antiphospholipid Syndrome: A Comprehensive Clinical Review
Authors: Patriarcheas Vasileios , Tsamos Georgios , Vasdeki Dimitra , Kotteas Elias , Kollias Anastasios , Nikas Dimitris , Kaiafa Georgia , Dimakakos Evangelos
Published: January 2025

Background: Antiphospholipid syndrome (APS) is a rare systemic autoimmune disease characterized by persistent antiphospholipid antibodies (aPL) in combination with recurrent thrombosis in the veins and/or arteries, obstetric morbidity, and various non-thrombotic associated complications. APS can be primary, as an isolated condition, or secondary in the context of another autoimmune disease, especially systemic lupus erythematosus. This comprehensive clinical review aims to summarize the current understanding of APS pathogenesis, diagnostic approaches, and treatment strategies for this unique clinical entity. Methods: A comprehensive review of the existing literature on APS was conducted, focusing on pathophysiological mechanisms, current diagnostic criteria, and therapeutic approaches. Results: APS pathogenesis involves complex interactions between aPL, phospholipid-binding proteins, and the coagulation cascade. Apart from the cardinal features of thrombosis and APS-related obstetric morbidity, APS is associated with a wide spectrum of clinical manifestations. Diagnosis remains challenging due to overlapping symptoms with other conditions, and clinicians should maintain a high index of suspicion in order to set the diagnosis. The recently published 2023 ACR/EULAR criteria although not definitive for clinical decision-making, these criteria offer clinicians a valuable tool to aid in determining whether further investigation for APS is warranted. Continued refinement of these criteria through ongoing feedback and updates is anticipated. Treatment strategies center on anticoagulation, but individualized approaches are necessary. Conclusions: Early diagnosis and multidisciplinary management of APS are critical to reducing morbidity and improving outcomes. Moreover, familiarization with the 2023 ACR/EULAR criteria is encouraged, recognizing that ongoing feedback and updates will contribute to their ongoing refinement and improvement. While VKAs remain the mainstay of treatment for most APS patients further research is needed to optimize treatment strategies and deepen our understanding of APS’s underlying disease mechanisms.
Development and Characterization of Cladding AISI 304L Stainless Steel on Aluminum
Authors: Gabsi Yasmine , Zouari Sahar , Abdennadher Mariem , Dieng Lamine , Elleuch Riadh
Published: January 2025

The cladding process is a cost-effective solution to improve surface properties and obtain additional functionalities. The current paper focuses on the cladding of austenitic 304L stainless steel on aluminum substrate (SS/Al) by diffusion bonding, particularly relevant in the kitchenware field for safety issues. The study investigates the bonding characteristics and the deep drawing formability of the clad material, aiming to improve the understanding of its performance. The main results show a defect-free interface, as observed through microstructural analyzes. This highlights the ability of this process to create a good bond. The SEM/EDS results confirmed the absence of a diffusion layer. Microhardness and adhesion tests revealed non-uniform hardness values and moderate strength values across the interface. Numerical simulation showed the feasibility of deep drawing the SS/Al clad material without failure, proving its suitability in cookware manufacturing. These findings demonstrate the workability of diffusion bonding and suggest the potential for improving mechanical properties.
The Antimicrobial Resistance of Candida: A 5-Year Retrospective Analysis at a Tertiary Hospital in Jordan
Authors: Abu-Humaidan Anas H. , Alshdaifat Areen , Awajan Dima , Abu-Hmidan Mohammad , Alshdifat Abeer , Hasan Hanan , Ahmad Fatima M. , Alaridah Nader , Irshaid Amal , Yamin Dina
Published: January 2025

Candida infections are a global health concern, increasingly complicated by rising antimicrobial resistance (AMR). This study analyzed the prevalence and AMR patterns of circulating Candida species in Amman, Jordan, using electronic records from a tertiary teaching hospital’s microbiology lab (from 2017 to 2022). Complete records of Candida isolates (n = 2673) were assessed by sample type, species, and AMR. Among positive blood samples, C. albicans accounted for the majority (38.7%), followed by C. tropicalis (19.0%), C. parapsilosis (18.3%), Nakaseomyces glabratus (14.6%), and Pichia kudriavzevii (9.5%). Non-albicans species demonstrated higher resistance to Caspofungin, notably P. kudriavzevii (23.1%), N. glabratus (30.0%), and C. parapsilosis (32.0%), compared to C. albicans (1.9%). In high vaginal swabs, C. albicans was most prevalent (63.7%), with N. glabratus also notable (28.6%); Fluconazole resistance in C. albicans remained low (2.0%). Across all pooled isolates, AMR was similar between inpatients and outpatients, except for Micafungin, where inpatient resistance was significantly higher. In conclusion, non-albicans species predominated in blood infections and demonstrated pronounced AMR. Micafungin resistance was notably higher among inpatients. Variations in Candida species and AMR by sample type suggest that aggregating samples in registry studies may obscure critical patterns.
Analysis of the Characteristics and Agglomeration Effect of the Rural Element Spatial Correlation Network in Northeast China
Authors: Sun Yu , Ning Jing , Piao Yongxin
Published: January 2025

In the face of the urgent need for the coordinated development of regional rural functions and the orderly and efficient integration of urban and rural areas, the problem of how to accurately identify the spatial correlation relationships and characteristics of rural elements among regions in Northeast China has become a key issue that urgently needs to be resolved. The results show the following: (1) The overall spatial correlation network (SCN) in the Northeast region from the perspective of rural element gravity has obvious differences. Each province has generated a strong connection center, and “strip-shaped” connection belts have been formed across provinces and cities. (2) From the perspective of the spatial pattern of the strong connection attributes of rural elements, Heilongjiang Province presents a polygonal “rhombus network”, Jilin Province presents a closed-loop “triangle network”, and Liaoning Province presents an irregular “trapezoid network”. (3) The connection relationships of rural element nodes within the provincial scope show that Yichun is an important hub connecting all directions within the province; Changchun and Siping have become the central nodes connecting the nodes on the northwest–southeast wings; Fuxin and Yingkou have become the central locations connecting the nodes on the southwest–northeast sides. (4) There are four sectors in the network, and the rural element transfer mechanism among the sectors shows that Block I and Block II are net spillover sectors, playing the role of “resource-based” sectors, and transmitting information to the net inflow Block IV through the broker Block III, presenting a “gradient” transmission mode.
Applicability of Statins in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)
Authors: Prasoppokakorn Thaninee
Published: January 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is the novel terminology encompassing liver disease associated with metabolic dysfunction, replacing the previous terminology of non-alcoholic fatty liver disease (NAFLD). This disease is strongly associated with metabolic disorders such as obesity, type 2 diabetes, and dyslipidemia. MASLD and dyslipidemia are deeply interconnected, driven by shared pathophysiological mechanisms. Emerging evidence suggests that statins, a class of lipid-lowering medications, may have beneficial effects on MASLD beyond their primary role in reducing cholesterol levels through several mechanisms, including anti-inflammatory, antioxidant, anti-fibrosis, and immunomodulatory effects. This review aims to summarize the efficacy of statins in the management of MASLD and provide insights into their potential mechanisms of action. It discusses the pathophysiology of MASLD and the role of statins in targeting key aspects of the disease. Additionally, the review examines the clinical evidence supporting the use of different statins in MASLD treatment and highlights their specific effects on liver enzymes, inflammation, and fibrosis. Furthermore, an algorithm for statin therapy in MASLD is proposed based on the current knowledge and available evidence.
Microeconomic Shock Propagation Through Production Networks in China
Authors: Liao Yihan
Published: January 2025

The question of whether microeconomic shocks induce aggregate fluctuations constitutes a central issue in economic research. This paper introduces a general equilibrium model with production networks to explore the propagation mechanisms of microeconomic shocks. A novel triangular production network structure is introduced, and simulations are performed using China’s input-output table to analyze the propagation of these shocks within the Chinese economy. The model demonstrates that the first-order effects of microeconomic shocks propagate downstream along the industrial chain, while the second-order effects of microeconomic productivity shocks propagate both upstream and downstream along the chain. The first-order propagation mechanism of microeconomic shocks involves changes in prices within the affected sector and its downstream sectors. Additionally, the second-order effects of microeconomic shocks rely on the reallocation of factors. The simulation results indicate that China’s production network matrix is triangular, and that the financial sector plays a crucial role in amplifying the effects of microeconomic shocks. Government should prioritize supporting upstream fundamental sectors to mitigate the adverse impacts of external shocks on economic fluctuations and to address systemic financial risks.
Reduction in Microgrid Topology Selection Time via Hybrid Branch and Bound and k-Nearest Neighbors Techniques
Authors: Legrene Inoussa , Wong Tony , Mary Nicolas , Dessaint Louis-A.
Published: January 2025

The global adoption of hybrid renewable energy systems (HRESs) is accelerating as a strategic response to escalating energy demands and the imperative to mitigate greenhouse gas emissions. Despite the development of various technological tools, such as pre-feasibility analysis, sizing, and simulation tools, challenges persist due to their limited flexibility in modifying system architectures and their typically long computation times, which hinder their practical efficiency. This study introduces a novel hybrid method that integrates the Branch and Bound (BB) heuristic search algorithm with the k-Nearest Neighbors (kNN) algorithm to drastically reduce the simulation time of microgrid models in Simulink. Validation considering four distinct case studies reveals that our method can decrease the simulation time by up to 94.68% while maintaining an acceptable accuracy. Specifically, simulation times in certain cases were reduced from approximately 21,780 and 118,580 s to 1442.7969 and 6306.0625 s, respectively. This significant reduction facilitates the rapid evaluation and selection of optimal HRES configurations, enhancing the efficiency of both editable and non-editable systems. Through streamlining the simulation process, this approach not only accelerates the design and analysis phases but also supports the broader adoption and deployment of HRESs, which is critical for achieving a sustainable future. This advancement offers a robust and efficient methodology for optimizing simulation times, thereby addressing a key bottleneck in the development and implementation of hybrid renewable energy solutions.
A Literature Review of Stochastic Modeling for Phylogenetic Comparative Analysis in Trait Evolution
Authors: Jhwueng Dwueng-Chwuan
Published: January 2025

Evolutionary inferences from phylogenetic trees can be modeled stochastically using a range of mathematical frameworks. Among these, stochastic differential equations (SDEs) provide a particularly flexible and powerful approach to capturing the continuous-time dynamics of evolutionary processes. This review summarizes advances in stochastic modeling for trait evolution along a phylogenetic tree, with a focus on stochastic differential equations (SDEs), Gaussian and non-Gaussian processes, and time series models that can be expressed as special cases of general stochastic frameworks, depending on the questions being addressed or the types of data analyzed. We explore current developments and future research directions of stochastic modeling for phylogenetic comparative analysis in trait evolution.
Efficient and Real-Time Compression Schemes of Multi-Dimensional Data from Ocean Buoys Using Golomb-Rice Coding
Authors: Liu Quan , Huang Ziling , Chen Kun , Xiao Jianmin
Published: January 2025

The energy supply of ocean monitoring buoys is a major challenge, especially for long-term, low-power applications. Data compression can reduce transmission energy and extend system lifespan. In particular, the algorithm cannot introduce delays to ensure real-time monitoring. In this scenario, we propose an efficient real-time compression scheme for lossless data compression (ERCS_Lossless) based on Golomb-Rice coding to efficiently compress each dimensional data independently. Additionally, we propose an efficient real-time compression scheme for lossy data compression with a flag mechanism (ERCS_Lossy_Flag), which incorporates a flag bit for each dimension, indicating if the prediction error exceeds a threshold, followed by further compression using Golomb-Rice coding. We conducted experiments on 24-dimensional weather and wave element data from a single buoy, and the results show that ERCS_Lossless achieves an average compression rate of 47.40%. In real communication scenarios, splicing and byte alignment operations are performed on multidimensional data, and the results show that the variance of the payload increases but the mean decreases after compression, realizing a 38.60% transmission energy saving, which is better than existing real-time lossless compression methods. In addition, ERCS_Lossy_Flag further reduces the amount of data and improves energy efficiency when lower data accuracy is acceptable.
On a New Class of Concave Bi-Univalent Functions Associated with Bounded Boundary Rotation
Authors: Sharma Prathviraj , Sivasubramanian Srikandan , Murugusundaramoorthy Gangadharan , Cho Nak Eun
Published: January 2025

In this research article, we introduce a new subclass of concave bi-univalent functions associated with bounded boundary rotation defined on an open unit disk. For this new class, we make an attempt to find the first two initial coefficient bounds. In addition, we investigate the very famous Fekete–Szegö inequality for functions belonging to this new subclass of concave bi-univalent functions related to bounded boundary rotation. For some particular choices of parameters, we derive the earlier estimates on the coefficient bounds, which are stated at the end.
An Assessment of the Effectiveness of Preoperative İmaging Modalities (MRI, CT, and 18F-FDG PET/CT) in Determining the Extent of Disease Spread in Epithelial Ovarian–Tubal–Peritoneal Cancer (EOC)
Authors: Kandemir Hülya , Sözen Hamdullah , Kartal Merve Gülbiz , Özkan Zeynep Gözde , Topuz Samet , Salihoğlu Mehmet Yavuz
Published: January 2025

Background and Objectives: Epithelial ovarian–tubal–peritoneal cancer (EOC) is the most common type of ovarian cancer. Optimal cytoreductive surgery is the most important prognostic factor in its management. When complete cytoreduction is anticipated to be challenging, neoadjuvant systemic chemotherapy (NACT) becomes an alternative. Imaging modalities are utilized in the decision-making process for primary treatment. The purpose of this study is to evaluate the diagnostic performance and accuracy of preoperative MRI, CT, and 18F-FDG PET/CT in detecting the extent of EOC. Materials and Methods: Between 2017 and 2018, 24 patients with primary (with or without neoadjuvant chemotherapy) or recurrent EOC diagnosed at the Department of Gynecologic Oncology, Istanbul University, Istanbul Faculty of Medicine, were enrolled in this study. These 24 women underwent preoperative imaging modalities within 7 days prior to surgery. The results were compared with histopathological findings, considered the gold standard. Results: We evaluated 24 anatomic regions most commonly involved in EOC. The sensitivity of MRI, CT, and PET/CT in detecting ≥ 0.5 cm implants was 95%, 84%, and 86%, respectively. However, when including implants < 0.5 cm, sensitivity decreased significantly to 40%, 38%, and 42%, respectively. The calculated area under the curve (AUC) for tumors, including those < 0.5 cm, was evaluated as weak for all three modalities (MRI: 0.689, CT: 0.678, PET/CT: 0.691), with PET/CT detecting the largest area. For detecting tumors ≥ 0.5 cm, the AUCs were 0.974, 0.921, and 0.923 for MRI, CT, and PET/CT, respectively. The largest AUC was calculated with MRI, and the AUCs for all three methods were evaluated as excellent. Accuracy was comparable among all three imaging modalities, and no statistically significant differences were found (p < 0.05). Conclusions: While imaging modalities are valuable tools for evaluating abdominal spread in epithelial ovarian cancer (EOC), they have demonstrated limited success in detecting miliary disease. The risk of false negatives for miliary tumors on PET/CT may be mitigated by combining it with other imaging modalities such as MRI or CT. Further investigations are necessary to identify more accurate imaging techniques for this challenging clinical scenario.
Synergistic Effects of Probiotics and Lifestyle Interventions on Intestinal Microbiota Composition and Clinical Outcomes in Obese Adults
Authors: Florêncio Glauber Pimentel , Xavier Analicy Rodrigues , Natal Ana Catarina de Castro , Sadoyama Lorena Prado , Röder Denise Von Dolinger de Brito , Menezes Ralciane de Paula , Sadoyama Leal Geraldo , Patrizzi Lislei Jorge , Pena Geórgia das Graças
Published: January 2025

Background and objective: Obesity is a growing global epidemic. The composition of the intestinal microbiota can be influenced by several factors. Studies highlight the role of intestinal bacteria in the pathophysiology of obesity. So, the objective of this study was to investigate whether the use of probiotics, together with healthy lifestyle habits, contributes to weight reduction in obese individuals by analyzing the intestinal microbiota profile. Methods: A prospective study was carried out with 45 adults with obesity. Participants underwent guidance on healthy lifestyle habits, received a probiotic component containing different microbiological strains and were followed for 60 days. Clinical parameters, body composition, biochemical analysis, and intestinal microbiota assessment were performed before and after treatment. After 60 days, it was observed that the bacterial strains present in the probiotic were present in the patients’ intestinal microbiota. Participants also showed improvements in physical activity, sleep quality, and anxiety management, as well as changes in some eating habits, such as a reduction in the consumption of processed foods and a significant increase in water intake. Results: A reduction in BMI, fasting glucose, insulin, HOMA-IR, LDL cholesterol, and triglycerides was observed, in addition to an increase in HDL cholesterol, improvement in bowel movement frequency, and stool consistency. Analysis of the intestinal microbiota revealed an increase in microbial diversity and a better balance between the bacterial phyla Firmicutes and Bacteroidetes. Conclusions: The changes related to improving the composition of the intestinal microbiota, dietary habits, increased physical activity, reduced anxiety, and better sleep quality have significantly contributed to weight loss and improvements in physiological parameters in obese individuals.
Effects of Acorns on Subcutaneous Fat Deposition in Yuxi Black Pigs by Transcriptomic Analysis
Authors: Sun Zhe , Liu Dongyang , An Siyuan , Zhang Jinzhou , Lei Lei , Miao Zhiguo
Published: January 2025

Background/Objectives: The backfat thickness of pigs is closely related to dorsal subcutaneous fat deposition and meat quality, and appropriate reduction in backfat thickness is important for improving pork quality. The present study investigated the effect of acorn diet on the backfat thickness and lipase activity of Yuxi black pigs and to gain further insight into the molecular mechanism of the acorn diet on the dorsal subcutaneous fat deposition of Yuxi black pigs by transcriptome sequencing (RNA-seq). Methods: Thirty-six Yuxi black pigs with an initial body weight of 99.60 ± 2.32 kg (three replicates per group and six pigs per replicate) were randomly divided into two groups (CON group was fed a basic diet and AEG group was fed 30% acorn diets). Pigs were individually fed twice daily and had access to water ad libitum throughout the experiment. The test period was 4 months. Results: Results showed that backfat thickness and ACC, MDH, and LPL lipase activities were significantly reduced in the AEG group than in the CON group (p < 0.05). In addition, RNA-seq identified 826 differentially expressed genes (DEGs), with 505 up-regulated and 321 down-regulated. The DEGs were significantly enriched in the lipid metabolism process and lipid catabolic process, fatty acid (FA) catabolic process, and FA β-oxidation according to GO enrichment analysis. LEP, CHPT1, UCP3, ACOX1, SCD5, and ACAA1 were screened as key differential genes regulating dorsal subcutaneous fat deposition. Conclusions: The above results indicated that feeding the 30% acorn diet could regulate the expression of genes involved in fat deposition and reduce lipase activity, thereby decreasing the backfat thickness, inhibiting the deposition of dorsal subcutaneous fat, and improving the pork quality. The findings of this experiment established a basis for subsequent research into the molecular mechanisms underlying the impact of acorn diets on fat deposition in Yuxi black pigs and provided the scientific evidence to promote the exploitation and industrialization of acorns.
Zoonotic Foodborne Infections in a Tertiary Healthcare Setting: Clinical and Epidemiological Aspects of Campylobacter and Salmonella Infection in Northern Portugal in 2010–2020
Authors: Matos Maria Inês , Rocha Rafael , Pinto João , Guimarães André , Lino Rita , Rocha Maria Helena , Patacho Marta , Duro Raquel , Santos Lurdes , Almeida Jorge
Published: January 2025

This study aimed to describe the epidemiology, clinical presentation, and management of Campylobacter sp. and Salmonella sp. infections in a tertiary hospital in Northern Portugal (2010–2020) and identify factors associated with reporting, invasive disease, and hospitalization. A retrospective review included patients with Campylobacter sp. (2015–2020) or Salmonella sp. infections (2010–2020). Reported cases were cross-referenced with the National Epidemiological Surveillance System. A total of 742 patients were included: 342 with Campylobacter sp., 392 with non-typhoidal Salmonella (NTS), and 15 with typhoidal Salmonella (TS). Immunosuppression was present in 16.7% of cases. NTS infections were invasive in 21.4% of cases, compared to 4.0% for Campylobacter. Macrolide resistance in Campylobacter reached 10%, particularly in C. coli, while multidrug resistance (MDR) was observed in 37.8% of NTS isolates. Immunocompromised patients accounted for over 70% of relapses in Campylobacter disease. Reporting rates were low: 32.8% for NTS, 30.6% for Campylobacter, and 14.3% for TS. Reporting was associated with male sex, no immunosuppression, hospitalization, and non-MDR organisms. In conclusion, immunocompromised patients face higher risks of invasive and relapsing disease. High rates of MDR in NTS limit treatment options. Underreporting remains significant, underscoring the need for improved awareness and reporting to inform public health strategies.
Petrography and Geochemistry of the Upper Cretaceous Volcaniclastic Deposits of the Haţeg Basin (Southern Carpathians): Inferences on Petrogenesis and Magma Origin
Authors: Vornicu Violeta M. , Seghedi Ioan
Published: January 2025

Upper Cretaceous volcaniclastic deposits of the Haţeg Basin (VDHB) (Southern Carpathians, Romania) consist of relatively poorly exposed products of multiple phreatomagmatic volcanic eruptions of andesitic to rhyolitic composition and crop out around Densuş, Răchitova, Peşteniţa, and Ciula Mică localities. These deposits are commonly associated with the Late Cretaceous Neotethyan magmatic activity that developed in Central-Eastern Europe, forming the Apuseni–Banat–Timok–Srednogorie (ABTS) belt. Since the geochemistry of these deposits has been investigated very little so far, this study provides petrographic and whole-rock geochemical analysis for twenty new different volcaniclastic rock samples, out of which sixteen samples represent lava clasts and the other four are samples of pyroclastic flow deposits. According to our geochemical data, the VDHB have a calc-alkaline and high-K calc-alkaline character, similar to the majority of rock samples from all sectors of the ABTS belt. A comparison between the Haţeg rock samples and Banat and Apuseni samples reveals comparable major and trace element abundances and REE patterns, supporting the idea that they originate from similar magmas. Trace element patterns suggest that the parental magmas were mostly derived from the melting of a metasomatized lithospheric mantle source, previously modified by an earlier subduction event. A combination of crystal fractionation and variable degrees of crustal assimilation during storage at higher and lower pressures was the principal mechanism driving calc-alkaline differentiation. Our geochemical analyses indicate that the VDHB were produced by magmas generated during two different magmatic events. Older, silica-rich melts produced the Peştenita and Răchitova ignimbrite deposits, while the Densuş and Răchitova andesitic–dacitic–rhyolitic rock suite was generated by younger, intermediate magmas. The individual melt production episodes are evidenced by the emergence of two different crystal fractionation trends: an amphibole-controlled trend at mid-crustal levels and an upper-crust plagioclase-dominated trend. The hydrous, calc-alkaline magmas arguably occurred in a post-collisional setting, in agreement with the orogenic collapse model, among others, proposed for the origin of the ABTS magmatic activity.
Research Progress Towards and Prospects of Carbon Dots Derived from Tea and Chinese Medicinal Materials
Authors: Tang Xiaoxue , Gong Zhao , Lang Yan , Chen Hongyue , Huang Siqi , Lv Yuguang
Published: January 2025

This review focuses on the research progress related to carbon dots (CDs) derived from Chinese herbal medicines and tea, covering preparation methods, physicochemical properties, and application fields. It elaborates on preparation approaches like hydrothermal, solvothermal, microwave-assisted, and ultrasonic-assisted methods, and their influence on CDs’ structure and properties. It also explores CDs’ structural and optical properties. The application fields include antibacterial, sensing, bioimaging, photocatalysis, hemostasis, and energy. Carbon dots show antibacterial activity by destroying bacterial cell membranes, they can detect various substances in sensing, are important for bioimaging, degrade organic pollutants in photocatalysis, have hemostatic and anti-inflammatory effects, and can be used as battery anode materials. Despite progress, challenges remain in improving yield, quantum yield, property control, and understanding their mechanism of action. This review provides a reference for related research and looks ahead to future directions.
Myricitrin Alleviates Hypercholesterolemia and Non-Alcoholic Fatty Liver Disease in High Cholesterol Diet-Fed Mice
Authors: Kim Young-Je , Park Sojeong , Kim HwiCheol , Kim Sang Ryong , Jung Un Ju
Published: January 2025

Background/Objectives: This research investigated the effects of myricitrin on hypercholesterolemia and non-alcoholic fatty liver disease (NAFLD) in mice given a high-cholesterol diet (HCD). Methods: C57BL/6J mice were maintained for 20 weeks on an HCD with or without myricitrin. Results: Myricitrin had no impact on the food consumption, body weight, or plasma triglyceride concentrations. However, myricitrin-supplemented mice had lower plasma total cholesterol (TC) concentrations and LDL + VLDL-cholesterol/TC proportion, and higher HDL-cholesterol/TC proportion than control mice, which resulted in a markedly decreased atherogenic index. Moreover, the levels of plasma C-reactive protein, oxidized LDL, lipoprotein(a), and plasminogen activator inhibitor-1, which are indicators for cardiovascular disease (CVD), were reduced, while levels of plasma paraoxonase, a cardioprotective enzyme, were greater in myricitrin-supplemented mice than in control mice. Myricitrin also meaningfully reduced liver weight and hepatic cholesterol content, and slightly alleviated fatty liver and fibrosis caused by an HCD. The plasma and hepatic cholesterol-lowering effects of myricitrin were partly associated with decreased activities of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase and acyl-CoA:cholesterol acyltransferase, which are involved in cholesterol synthesis and esterification, respectively, as well as mRNA expression. Myricitrin also altered other hepatic genes implicated in cholesterol homeostasis, including the downregulation of SREBP2 and ABCA1 mRNA expression and the upregulation of LDLR mRNA expression. Moreover, myricitrin decreased TBARS levels in the liver and erythrocytes by activating antioxidant enzymes (SOD and catalase). Conclusions: These results indicate that dietary myricitrin may offer therapeutic benefits for HCD-caused hypercholesterolemia and NAFLD, and may help reduce CVD risk.
Effects of a Novel Dispersible Supplement Containing 2500 IU of Vitamin D and 1000 µg of B12 in Restoring Vitamin D and B12 Insufficiency: A Multicenter, Randomized Controlled Trial
Authors: Angelopoulos Nikolaos , Paparodis Rodis D. , Androulakis Ioannis , Boniakos Anastasios , Livadas Sarantis
Published: January 2025

Background/Objectives: Vitamins D and B12 play a crucial role in maintaining bone health, immune function, and neurological integrity. Combined deficiencies in these vitamins can lead to severe health consequences. Current treatment approaches, such as dietary changes and single-vitamin supplementation, often fail to address these deficiencies comprehensively. This study evaluates the effectiveness of concurrent vitamin D and B12 supplementation to correct these insufficiencies. Methods: A prospective, multicenter, randomized controlled trial was conducted in Greece from October 2024 to December 2024. Participants aged 20 to 80 years, with insufficient levels of 25-hydroxyvitamin D (serum < 20 ng/mL) and B12 (serum < 250 ng/L), were eligible for inclusion. Results: A total of 124 patients were randomized into three groups: one receiving vitamins B12 and D in a single supplement (2500 IU + 1000 mcg), one receiving separate doses of each vitamin (2000 IU + 1000 mcg), and a control group receiving no supplementation. The results demonstrated a significant increase in B12 and 25-hydroxyvitamin D levels among the supplemented groups. Particularly, participants in the combined supplementation group showed higher average serum levels of both vitamins. By the end of this study, 37.1% of those in the combined supplement group achieved adequate vitamin levels, compared to 29.4% in the separate supplementation group. Conclusions: In conclusion, combined supplementation may improve patient adherence and compliance, leading to better health outcomes for individuals with combined vitamins D and B12 deficiencies.
Realization of the Compact Magneto-Optical Trap Based on Single Laser with Frequency Modulation
Authors: Bao Shuning , Wu Bin , Cao Pengfei , Guo Xiaochun , Zhao Yingpeng , Li Dianrong , Niu Jingyu , Zhang Cheng , Cheng Bing , Weng Kanxing , Wang Xiaolong , Lin Qiang
Published: January 2025

In cold atom physics, the complexity of traditional magneto-optical trap system limits the use of their associated instruments for field applications in atomic physics, such as gravity mapping, space navigation and deep space exploration. This study introduces a novel compact MOT design that addresses these issues by simplifying the structure and reducing the size. The height of the unit is 0.7 m, the volume is 6.3×10−2m3 and the mass is 11.32 kg. The new design utilizes a single laser to generate the two different frequencies needed for laser cooling by internally splitting the beam, shifting the frequency and then combining them, effectively controlling both the cooling and repumping beams. The compact vacuum chamber optical path, in conjunction with the magnetic field, facilitates the capture of 87Rb atoms in an ultra-high vacuum environment. Experimental results demonstrate an atom loading rate of up to 1.79×10787Rb atoms per second, confirming the system’s effectiveness in capturing and cooling 87Rb atoms. This design provides a flexible and portable solution, offering valuable insights for the advancement of compact MOT and its applications in cold atom physics.
Integration of p-Type PdPc and n-Type SnZnO into Hybrid Nanofibers Using Simple Chemical Route for Enhancement of Schottky Diode Efficiency
Authors: Al-Sayed A. , Siddiq Miad Ali , Elgazzar Elsayed
Published: January 2025

Palladium phthalocyanine (PdPc) and palladium phthalocyanine integrated with tin–zinc oxide (PdPc:SnZnO) were prepared using a simple chemical approach, and their structural and morphological properties were identified using X-ray diffraction, energy dispersive X-ray analysis, scanning electron microscopy, and transmission electron microscopy techniques. The PdPc:SnZnO nanohybrid revealed a polycrystalline structure combining n-type metal oxide SnZnO nanoparticles with p-type organic PdPc molecules. The surface morphology exhibited wrinkled nanofibers decorated with tiny spheres and had a large aspect ratio. The thin film revealed significant optical absorption within the ultraviolet and visible spectra, with narrow band gaps measured at 1.52 eV and 2.60 eV. The electronic characteristics of Al/n-Si/PdPc/Ag and Al/n-Si/PdPc:SnZnO/Ag Schottky diodes were investigated using the current–voltage dependence in both the dark conditions and under illumination. The photodiodes displayed non-ideal behavior with an ideality factor greater than unity. The hybrid diode showed considerably high rectification ratio of 899, quite a low potential barrier, substantial specific photodetectivity, and high enough quantum efficiency, found to be influenced by dopant atoms and the unique topological architecture of the nanohybrid. The capacitance/conductance–voltage dependence measurements revealed the influence of alternative current signals on trapped centers at the interface state, leading to an increase in charge carrier density.
Combined Effect of Temperature and Different Light Regimes on the Photosynthetic Activity and Lipid Accumulation in the Diatom Phaeodactylum tricornutum
Authors: Díaz-Santos Encarnación , Heredia-Martínez Luis G. , López-Maury Luis , Hervás Manuel , Ortega José M. , Navarro José A. , Roncel Mercedes
Published: January 2025

The aim of this study was to investigate the combined effects of temperature and light on the photosynthetic parameters and lipid accumulation in the diatom Phaeodactylum tricornutum, a model organism widely used for studies on diatom physiology, ecology, and biotechnology. Our results highlight the importance of the interaction between temperature and light intensity in influencing growth rates, pigments and active photosystems content, photosynthetic efficiency, lipid production and fatty acid composition in P. tricornutum. Measurements of the maximum electron transport rate (rETRmax) and rETR at maximum PAR (830 µmol m−2 s−1) confirmed that P. tricornutum exhibits significantly higher light sensitivity as growth temperature increases under light/dark cycles at two light intensities (25–60 µmol m−2 s−1). However, this trend was reversed under continuous light (25 µmol m−2 s−1). Moreover, higher rETRmax values (up to double) were observed at higher irradiance, either in intensity or under continuous light regimes, at the two temperatures tested. On the other hand, increasing light intensity amplified the observed effect of temperature on photosystem I (PSI) activity under light/dark regimes, but not under continuous light conditions. This resulted in a greater deficiency in PSI activity, likely due to limitations in electron supply to this photosystem. Furthermore, increasing the culture temperature from 20 °C to 25 °C triggered an increase in the number and size of cytoplasmic lipid droplets under conditions of increased light intensity, with an even more pronounced effect under continuous illumination. Notably, the combination of 25 °C and continuous illumination resulted in a more than twofold increase in triacylglyceride (TAG) content, reaching approximately 17 mg L−1. This condition also caused a substantial rise (up to ≈90%) in the proportions of palmitoleic and palmitic acids in the TAG fatty acid profile.