bluuebunny/crossref_metadata_2025 huggingface dataset:
On Vernon Lee's Walter Pater and Translating the Victorians
Authors: Valentine Colton
Published: May 2023

Vernon Lee (Violet Paget) (1856–1935) first met Walter Pater (1839–1894) at Oxford during the summer of 1881, though she was already well acquainted with his work. The extent and import of their relationship, which lasted until Pater's death, has received significant attention by biographers and critics.1 Like many of Lee's friendships, its intimacies were both personal and intellectual—risking, as she put it, “a question of caw-me-caw-you” through reciprocal endorsement.2 The epistolary-averse Pater was uncharacteristically forthcoming in their correspondence, and Lee often stayed with his family when visiting England. The two read aloud from drafts, exchanged books, and met repeatedly on the published page. Indeed, scholars have traced an elegant intertextual arc from Lee's rewriting of Pater's “The Child in the House” (1878) in Belcaro (1881), to her dedication of Euphorion (1884), through her introduction to Juvenilia (1887), and arriving at her “Valedictory” conclusion to Renaissance Fancies and Studies (1895).3 By this account, Lee initially adheres to Paterian aesthetics, then grows skeptical of the doctrine's epicurean features, but finds solace when her mentor's late work takes its own ethical turn.
Synoptic Images: Truth and Temporality in Pictorial Journalism During the 1840s
Authors: Kihlberg Jakob
Published: May 2023

The 1840s saw the creation of weekly illustrated newsmagazines in several European countries, with titles like the Illustrated London News, L'Illustration, and the Illustrirte Zeitung. This article questions the common assumption that these influential periodicals contributed to modern news time primarily by speeding up the consumption of visual information through rapidly produced and consumed eyewitness accounts. An analysis of the many images of staged public events, like inaugurations, political meetings, and parades, that were published during the early years of illustrated news instead shows than they were often produced as translations of information from different sources (both visual and verbal) and created as pictorial summaries of events in a way that can best be described as “synoptic.” Rather than ephemeral reflections of an ever-changing present, such visual condensations also served to commemorate and make lasting and thus contributed to establishing the very events on which the experience of a fast-forward movement of time was based.
Concentration and Isotopic Composition of Atmospheric N2O Over the Last Century
Authors: Ghosh Sambit , Toyoda Sakae , Buizert Christo , Etheridge David M. , Langenfelds Ray L. , Yoshida Naohiro , Kim Seong‐Joong , Ahn Jinho
Published: May 2023

Temporal changes in the magnitude and geographic distribution of different sources of nitrous oxide (N2O) are not well constrained. To better understand the dynamics of N2O in the atmosphere over the last century, we have reconstructed the mole fraction, δ15Nbulk, δ18O, and δ15NSP values of N2O from ice cores, firn air archives, and modern atmospheric samples. We have provided new firn air records from the Styx Glacier, Antarctica, and the North Greenland Eemian Ice drilling Project, and updated the firn air transport modeling of the published records. The composite reconstruction shows that the N2O growth rates were 0.26 ± 0.05, 0.15 ± 0.05 and 0.75 ± 0.01 ppb yr−1 during 1850–1930 (P1), 1931–1965 (P2) and 1966–2021 CE (P3), respectively. The temporal slope found in a linear least squares fit in δ15Nbulk and δ18O were −0.010 ± 0.025 and −0.004 ± 0.031‰ yr−1, −0.014 ± 0.013 and −0.009 ± 0.017‰ yr−1, and −0.040 ± 0.013 and −0.022 ± 0.005‰ yr−1 during P1, P2 and P3 phases, respectively. Overall, a significant long‐term trend was not observed in δ15NSP data. Two‐box model calculations using N2O mole fraction suggest that the total N2O flux (FT) at 2015 CE was 17.5 ± 1.1 TgN yr−1, where flux from the natural (FN) and anthropogenic (FA) sources were ∼60% and 40% of FT, respectively, and the contribution of FA was ∼30% of FT at 1900 CE. Estimated FA and δ15Nbulk of atmospheric N2O suggest that the anthropogenic emissions from continental regions were 12%, 25% and 76% of FA during P1, P2 and P3 phases, respectively.
Fast Secondary Aerosol Formation in Residual Layer and Its Impact on Air Pollution Over Eastern China
Authors: Zhou Xueyu , Huang Xin , Sun Peng , Chi Xuguang , Ren Chuanhua , Lai Shiyi , Wang Zilin , Qi Ximeng , Wang Jiaping , Nie Wei , Xu Zheng , Huo Juntao , Fu Qinyan , Ding Aijun
Published: May 2023

Though China's air quality has been improved in the past decade, haze still engulfs megacities in winter with fast increasing secondary aerosol like nitrate. Given that aerosol and its precursors exhibit great vertical heterogeneity, we conducted airship measurements at Shanghai in December 2017 to understand the critical drivers for the rapidly increased secondary pollution. By integrating in‐situ observations and model simulations, we found that cold front favors the long‐range transport of pollutants, during which chemical formation of nitrate in residual layer was faster than near the surface due to concentrated precursors and sufficient oxidation. The nitrate aloft would be entrained down once the daytime convective boundary layer develops, thereby deteriorating near‐surface air pollution. It is shown that the local surface‐level chemical production played a minor role, whereas the synoptic weather and boundary layer evolution largely contributed to enhanced secondary aerosol pollution (>60%). This study demonstrates that weather systems, boundary layer evolution, and chemical processes could jointly shape secondary aerosol production, highlighting the importance of a vertical understanding of haze pollution.
Probabilistic Forecasting of Ground Magnetic Perturbation Spikes at Mid‐Latitude Stations
Authors: Coughlan Michael , Keesee Amy , Pinto Victor , Mukundan Raman , Marchezi José Paulo , Johnson Jeremiah , Connor Hyunju , Hampton Don
Published: May 2023

The prediction of large fluctuations in the ground magnetic field (dB/dt) is essential for preventing damage from Geomagnetically Induced Currents. Directly forecasting these fluctuations has proven difficult, but accurately determining the risk of extreme events can allow for the worst of the damage to be prevented. Here we trained Convolutional Neural Network models for eight mid‐latitude magnetometers to predict the probability that dB/dt will exceed the 99th percentile threshold 30–60 min in the future. Two model frameworks were compared, a model trained using solar wind data from the Advanced Composition Explorer (ACE) satellite, and another model trained on both ACE and SuperMAG ground magnetometer data. The models were compared to examine if the addition of current ground magnetometer data significantly improved the forecasts of dB/dt in the future prediction window. A bootstrapping method was employed using a random split of the training and validation data to provide a measure of uncertainty in model predictions. The models were evaluated on the ground truth data during eight geomagnetic storms and a suite of evaluation metrics are presented. The models were also compared to a persistence model to ensure that the model using both datasets did not over‐rely on dB/dt values in making its predictions. Overall, we find that the models using both the solar wind and ground magnetometer data had better metric scores than the solar wind only and persistence models, and was able to capture more spatially localized variations in the dB/dt threshold crossings.
Dose-efficient scanning Compton X-ray microscopy
Authors: Li Tang , Dresselhaus J. Lukas , Ivanov Nikolay , Prasciolu Mauro , Fleckenstein Holger , Yefanov Oleksandr , Zhang Wenhui , Pennicard David , Dippel Ann-Christin , Gutowski Olof , Villanueva-Perez Pablo , Chapman Henry N. , Bajt Saša
Published: May 2023

The highest resolution of images of soft matter and biological materials is ultimately limited by modification of the structure, induced by the necessarily high energy of short-wavelength radiation. Imaging the inelastically scattered X-rays at a photon energy of 60 keV (0.02 nm wavelength) offers greater signal per energy transferred to the sample than coherent-scattering techniques such as phase-contrast microscopy and projection holography. We present images of dried, unstained, and unfixed biological objects obtained by scanning Compton X-ray microscopy, at a resolution of about 70 nm. This microscope was realised using novel wedged multilayer Laue lenses that were fabricated to sub-ångström precision, a new wavefront measurement scheme for hard X rays, and efficient pixel-array detectors. The doses required to form these images were as little as 0.02% of the tolerable dose and 0.05% of that needed for phase-contrast imaging at similar resolution using 17 keV photon energy. The images obtained provide a quantitative map of the projected mass density in the sample, as confirmed by imaging a silicon wedge. Based on these results, we find that it should be possible to obtain radiation damage-free images of biological samples at a resolution below 10 nm.
Predicting recurrence of depression using cardiac complexity in individuals tapering antidepressants
Authors: George Sandip V. , Kunkels Yoram K. , Smit Arnout , Wichers Marieke , Snippe Evelien , van Roon Arie M. , Riese Harriëtte
Published: May 2023

It is currently unknown whether the complexity and variability of cardiac dynamics predicts future depression and whether within-subject change herein precedes the recurrence of depression. We tested this in an innovative repeated single-subject study in individuals who had a history of depression and were tapering their antidepressants. In 50 individuals, electrocardiogram (ECG) derived Interbeat-interval (IBI) time-series data were collected for 5 min every morning and evening, for 4 months. Usable data were obtained from 14 participants who experienced a transition (i.e., a clinically significant increase in depressive symptoms) and 14 who did not. The mean, standard deviation, Higuchi dimension and multiscale entropy, calculated from IBIs, were examined for time trends. These quantifiers were also averaged over a baseline period and compared between the groups. No consistent trends were observed in any quantifier before increases in depressive symptoms within individuals. The entropy baseline levels significantly differed between the two groups (morning: P value < 0.001, Cohen’s d = −2.185; evening: P value < 0.001, Cohen’s d = −1.797) and predicted the recurrence of depressive symptoms, in the current sample. Moreover, higher mean IBIs and Higuchi dimensions were observed in individuals who experienced transitions. While we found little evidence to support the existence of within- individual warning signals in IBI time-series data preceding an upcoming depressive transition, our results indicate that individuals who taper antidepressants and showed lower entropy of cardiac dynamics exhibited a higher chance of recurrence of depression. Hence, entropy could be a potential digital phenotype for assessing the risk of recurrence of depression in the short term while tapering antidepressants.
Macrophage phagocytosis of SARS-CoV-2-infected cells mediates potent plasmacytoid dendritic cell activation
Authors: García-Nicolás O. , Godel A. , Zimmer G. , Summerfield A.
Published: May 2023

Early and strong interferon type I (IFN-I) responses are usually associated with mild COVID-19 disease, whereas persistent or unregulated proinflammatory cytokine responses are associated with severe disease outcomes. Previous work suggested that monocyte-derived macrophages (MDMs) are resistant and unresponsive to SARS-CoV-2 infection. Here, we demonstrate that upon phagocytosis of SARS-CoV-2-infected cells, MDMs are activated and secrete IL-6 and TNF. Importantly, activated MDMs in turn mediate strong activation of plasmacytoid dendritic cells (pDCs), leading to the secretion of high levels of IFN-α and TNF. Furthermore, pDC activation promoted IL-6 production by MDMs. This kind of pDC activation was dependent on direct integrin-mediated cell‒cell contacts and involved stimulation of the TLR7 and STING signaling pathways. Overall, the present study describes a novel and potent pathway of pDC activation that is linked to the macrophage-mediated clearance of infected cells. These findings suggest that a high infection rate by SARS-CoV-2 may lead to exaggerated cytokine responses, which may contribute to tissue damage and severe disease.
Hydrogel-in-hydrogel live bioprinting for guidance and control of organoids and organotypic cultures
Authors: Urciuolo Anna , Giobbe Giovanni Giuseppe , Dong Yixiao , Michielin Federica , Brandolino Luca , Magnussen Michael , Gagliano Onelia , Selmin Giulia , Scattolini Valentina , Raffa Paolo , Caccin Paola , Shibuya Soichi , Scaglioni Dominic , Wang Xuechun , Qu Ju , Nikolic Marko , Montagner Marco , Galea Gabriel L. , Clevers Hans , Giomo Monica , De Coppi Paolo , Elvassore Nicola
Published: May 2023

Three-dimensional hydrogel-based organ-like cultures can be applied to study development, regeneration, and disease in vitro. However, the control of engineered hydrogel composition, mechanical properties and geometrical constraints tends to be restricted to the initial time of fabrication. Modulation of hydrogel characteristics over time and according to culture evolution is often not possible. Here, we overcome these limitations by developing a hydrogel-in-hydrogel live bioprinting approach that enables the dynamic fabrication of instructive hydrogel elements within pre-existing hydrogel-based organ-like cultures. This can be achieved by crosslinking photosensitive hydrogels via two-photon absorption at any time during culture. We show that instructive hydrogels guide neural axon directionality in growing organotypic spinal cords, and that hydrogel geometry and mechanical properties control differential cell migration in developing cancer organoids. Finally, we show that hydrogel constraints promote cell polarity in liver organoids, guide small intestinal organoid morphogenesis and control lung tip bifurcation according to the hydrogel composition and shape.
Transcriptional coactivation by EHMT2 restricts glucocorticoid-induced insulin resistance in a study with male mice
Authors: Lee Rebecca A. , Chang Maggie , Yiv Nicholas , Tsay Ariel , Tian Sharon , Li Danielle , Poulard Coralie , Stallcup Michael R. , Pufall Miles A. , Wang Jen-Chywan
Published: May 2023

The classical dogma of glucocorticoid-induced insulin resistance is that it is caused by the transcriptional activation of hepatic gluconeogenic and insulin resistance genes by the glucocorticoid receptor (GR). Here, we find that glucocorticoids also stimulate the expression of insulin-sensitizing genes, such as Irs2. The transcriptional coregulator EHMT2 can serve as a transcriptional coactivator or a corepressor. Using male mice that have a defective EHMT2 coactivation function specifically, we show that glucocorticoid-induced Irs2 transcription is dependent on liver EHMT2’s coactivation function and that IRS2 play a key role in mediating the limitation of glucocorticoid-induced insulin resistance by EHMT2’s coactivation. Overall, we propose a model in which glucocorticoid-regulated insulin sensitivity is determined by the balance between glucocorticoid-modulated insulin resistance and insulin sensitizing genes, in which EHMT2 coactivation is specifically involved in the latter process.
The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes
Authors: Wu Shuangcheng Alivia , Shen Chenchen , Wei Xiaoqiong , Zhang Xiawei , Wang Siwen , Chen Xinxin , Torres Mauricio , Lu You , Lin Liangguang Leo , Wang Huilun Helen , Hunter Allen H. , Fang Deyu , Sun Shengyi , Ivanova Magdalena I. , Lin Yi , Qi Ling
Published: May 2023

Endoplasmic reticulum (ER)-associated degradation (ERAD) and ER-phagy are two principal degradative mechanisms for ER proteins and aggregates, respectively; however, the crosstalk between these two pathways under physiological settings remains unexplored. Using adipocytes as a model system, here we report that SEL1L-HRD1 protein complex of ERAD degrades misfolded ER proteins and limits ER-phagy and that, only when SEL1L-HRD1 ERAD is impaired, the ER becomes fragmented and cleared by ER-phagy. When both are compromised, ER fragments containing misfolded proteins spatially coalesce into a distinct architecture termed Coalescence of ER Fragments (CERFs), consisted of lipoprotein lipase (LPL, a key lipolytic enzyme and an endogenous SEL1L-HRD1 substrate) and certain ER chaperones. CERFs enlarge and become increasingly insoluble with age. Finally, we reconstitute the CERFs through LPL and BiP phase separation in vitro, a process influenced by both redox environment and C-terminal tryptophan loop of LPL. Hence, our findings demonstrate a sequence of events centered around SEL1L-HRD1 ERAD to dispose of misfolded proteins in the ER of adipocytes, highlighting the profound cellular adaptability to misfolded proteins in the ER in vivo.
Newborn metabolomic signatures of maternal per- and polyfluoroalkyl substance exposure and reduced length of gestation
Authors: Taibl Kaitlin R. , Dunlop Anne L. , Barr Dana Boyd , Li Yuan-Yuan , Eick Stephanie M. , Kannan Kurunthachalam , Ryan P. Barry , Schroder Madison , Rushing Blake , Fennell Timothy , Chang Che-Jung , Tan Youran , Marsit Carmen J. , Jones Dean P. , Liang Donghai
Published: May 2023

Marginalized populations experience disproportionate rates of preterm birth and early term birth. Exposure to per- and polyfluoroalkyl substances (PFAS) has been reported to reduce length of gestation, but the underlying mechanisms are unknown. In the present study, we characterized the molecular signatures of prenatal PFAS exposure and gestational age at birth outcomes in the newborn dried blood spot metabolome among 267 African American dyads in Atlanta, Georgia between 2016 and 2020. Pregnant people with higher serum perfluorooctanoic acid and perfluorohexane sulfonic acid concentrations had increased odds of an early birth. After false discovery rate correction, the effect of prenatal PFAS exposure on reduced length of gestation was associated with 8 metabolomic pathways and 52 metabolites in newborn dried blood spots, which suggested perturbed tissue neogenesis, neuroendocrine function, and redox homeostasis. These mechanisms explain how prenatal PFAS exposure gives rise to the leading cause of infant death in the United States.
Cross-linked polyaniline for production of long lifespan aqueous iron||organic batteries with electrochromic properties
Authors: Lv Haiming , Wei Zhiquan , Han Cuiping , Yang Xiaolong , Tang Zijie , Zhang Yantu , Zhi Chunyi , Li Hongfei
Published: May 2023

Aqueous iron batteries are appealing candidates for large-scale energy storage due to their safety and low-cost aspects. However, the development of aqueous Fe batteries is hindered by their inadequate long-term cycling stability. Here, we propose the synthesis and application as positive electrode active material of cross-linked polyaniline (C-PANI). We use melamine as the crosslinker to improve the electronical conductivity and electrochemical stability of the C-PANI. Indeed, when the C-PANI is tested in combination with a Fe metal negative electrode and 1 M iron trifluoromethanesulfonate (Fe(TOF)2) electrolyte solution, the coin cell can deliver a specific capacity of about 110 mAh g−1 and an average discharge voltage of 0.55 V after 39,000 cycles at 25 A g−1 with a test temperature of 28 °C ± 1 °C. Furthermore, mechanistic studies suggest that Fe2+ ions are bonded to TOF− anions to form positively charged complexes Fe(TOF)+, which are stored with protons in the C-PANI electrode structures. Finally, we also demonstrate the use of C-PANI in combination with a polymeric hydrogel electrolyte to produce a flexible reflective electrochromic lab-scale iron battery prototype.
Semiconducting MOFs on ultraviolet laser-induced graphene with a hierarchical pore architecture for NO2 monitoring
Authors: Lim Hyeongtae , Kwon Hyeokjin , Kang Hongki , Jang Jae Eun , Kwon Hyuk-Jun
Published: May 2023

Due to rapid urbanization worldwide, monitoring the concentration of nitrogen dioxide (NO2), which causes cardiovascular and respiratory diseases, has attracted considerable attention. Developing real-time sensors to detect parts-per-billion (ppb)-level NO2 remains challenging due to limited sensitivity, response, and recovery characteristics. Herein, we report a hybrid structure of Cu3HHTP2, 2D semiconducting metal-organic frameworks (MOFs), and laser-induced graphene (LIG) for high-performance NO2 sensing. The unique hierarchical pore architecture of LIG@Cu3HHTP2 promotes mass transport of gas molecules and takes full advantage of the large surface area and porosity of MOFs, enabling highly rapid and sensitive responses to NO2. Consequently, LIG@Cu3HHTP2 shows one of the fastest responses and lowest limit of detection at room temperature compared with state-of-the-art NO2 sensors. Additionally, by employing LIG as a growth platform, flexibility and patterning strategies are achieved, which are the main challenges for MOF-based electronic devices. These results provide key insight into applying MOFtronics as high-performance healthcare devices.
On the effect of Al on alumino-borosilicate glass chemical durability
Authors: Damodaran Kamalesh , Gin Stéphane , Narayanasamy Sathya , Delaye Jean-Marc
Published: May 2023

The chemical durability of borosilicate glass used to confine nuclear wastes is known to vary nonlinearly with their composition, making glass dissolution rate predictions difficult. Here, we focus on the effect of Al, an important oxide of these materials. The initial and residual glass dissolution rates were investigated through experiments conducted at 90 °C and pH 9. Our results show that low Al content glasses dissolve initially faster than glasses with higher Al content, but quickly achieve a lower residual rate due to the rapid formation of a more passivating gel. Nevertheless, the high Al content gel will eventually achieve passivation with a slower reorganization. The strengthening effect of Al on the Si-O bond hydrolysis will result in more stable gels, suggesting that slightly higher Al content in the gel will improve nuclear high-level waste glass durability in geological disposal conditions.
Immunization against arthropod protein impairs transmission of rickettsial pathogen from ticks to the vertebrate host
Authors: Mahesh P. P. , Namjoshi Prachi , Sultana Hameeda , Neelakanta Girish
Published: May 2023

Human anaplasmosis caused by Anaplasma phagocytophilum is one of the most common tick-borne diseases in the United States. The black-legged ticks, Ixodes scapularis, vector and transmit this bacterium to humans. In this study, we provide evidence that targeting I. scapularis membrane-bound organic anion transporting polypeptide 4056 (IsOATP4056) with an anti-vector vaccine affects transmission of A. phagocytophilum from ticks to the vertebrate host. Anaplasma phagocytophilum induces expression of IsOATP4056 in ticks and tick cells. Increased membrane localization of IsOATP4056 was evident in A. phagocytophilum-infected tick cells. Treatment with high dose (10 µg/ml) but not low dose (5 µg/ml) of EL-6 antibody that targets the largest extracellular loop of IsOATP4056 showed cytotoxic effects in tick cells but not in human keratinocyte cell line (HaCaT). Passive immunization, tick-mediated transmission and in vitro studies performed with mice ordered from two commercial vendors and with tick cells showed that EL-6 antibody not only impairs A. phagocytophilum transmission from ticks to the murine host but also aids in the reduction in the bacterial loads within engorged ticks and in tick cells by activation of arthropod Toll pathway. Furthermore, reduced molting efficiency was noted in ticks fed on EL-6 antibody-immunized mice. Collectively, these results provide a good candidate for the development of anti-tick vaccine to target the transmission of A. phagocytophilum and perhaps other tick-borne pathogens of medical importance.
Breast cancer prevention with liquiritigenin from licorice through the inhibition of aromatase and protein biosynthesis in high-risk women’s breast tissue
Authors: Hajirahimkhan Atieh , Howell Caitlin , Bartom Elizabeth T. , Dong Huali , Lantvit Daniel D. , Xuei Xiaoling , Chen Shao-Nong , Pauli Guido F. , Bolton Judy L. , Clare Susan E. , Khan Seema A. , Dietz Birgit M.
Published: May 2023

Breast cancer risk continues to increase post menopause. Anti-estrogen therapies are available to prevent postmenopausal breast cancer in high-risk women. However, their adverse effects have reduced acceptability and overall success in cancer prevention. Natural products such as hops (Humulus lupulus) and three pharmacopeial licorice (Glycyrrhiza) species have demonstrated estrogenic and chemopreventive properties, but little is known regarding their effects on aromatase expression and activity as well as pro-proliferation pathways in human breast tissue. We show that Gycyrrhiza inflata (GI) has the highest aromatase inhibition potency among these plant extracts. Moreover, phytoestrogens such as liquiritigenin which is common in all licorice species have potent aromatase inhibitory activity, which is further supported by computational docking of their structures in the binding pocket of aromatase. In addition, GI extract and liquiritigenin suppress aromatase expression in the breast tissue of high-risk postmenopausal women. Although liquiritigenin has estrogenic effects in vitro, with preferential activity through estrogen receptor (ER)-β, it reduces estradiol-induced uterine growth in vivo. It downregulates RNA translation, protein biosynthesis, and metabolism in high-risk women’s breast tissue. Finally, it reduces the rate of MCF-7 cell proliferation, with repeated dosing. Collectively, these data suggest that liquiritigenin has breast cancer prevention potential for high-risk postmenopausal women.
Correlating the differences in the receptor binding domain of SARS-CoV-2 spike variants on their interactions with human ACE2 receptor
Authors: Mahalingam Gokulnath , Arjunan Porkizhi , Periyasami Yogapriya , Dhyani Ajay Kumar , Devaraju Nivedita , Rajendiran Vignesh , Christopher Abisha Crystal , KT Ramya Devi , Dhanasingh Immanuel , Thangavel Saravanabhavan , Murugesan Mohankumar , Moorthy Mahesh , Srivastava Alok , Marepally Srujan
Published: May 2023

Spike glycoprotein of SARS-CoV-2 variants plays a critical role in infection and transmission through its interaction with human angiotensin converting enzyme 2 (hACE2) receptors. Prior findings using molecular docking and biomolecular studies reported varied findings on the difference in the interactions among the spike variants with the hACE2 receptors. Hence, it is a prerequisite to understand these interactions in a more precise manner. To this end, firstly, we performed ELISA with trimeric spike glycoproteins of SARS-CoV-2 variants including Wuhan Hu-1(Wild), Delta, C.1.2 and Omicron. Further, to study the interactions in a more specific manner by mimicking the natural infection, we developed hACE2 receptors expressing HEK-293T cell line, evaluated their binding efficiencies and competitive binding of spike variants with D614G spike pseudotyped virus. In line with the existing findings, we observed that Omicron had higher binding efficiency compared to Delta in both ELISA and Cellular models. Intriguingly, we found that cellular models could differentiate the subtle differences between the closely related C.1.2 and Delta in their binding to hACE2 receptors. Our study using the cellular model provides a precise method to evaluate the binding interactions between spike sub-lineages to hACE2 receptors.
Insulin-like growth factor binding protein-3 induces senescence by inhibiting telomerase activity in MCF-7 breast cancer cells
Authors: Kwon Ahreum , Chae Hyun Wook , Lee Woo Jung , Kim JungHyun , Kim Ye Jin , Ahn Jungmin , Oh Youngman , Kim Ho-Seong
Published: May 2023

Insulin-like growth factor binding protein-3 (IGFBP-3) has been known to inhibit cell proliferation and exert tumor-suppressing effects depending on the cell type. In this study, we aimed to show that IGFBP-3 induces cellular senescence via suppression of the telomerase activity, thereby inhibiting MCF-7 breast cancer cell proliferation. We found that the induction of IGFBP-3 in MCF-7 cells enhanced the loss of cell viability. Flow cytometry revealed a higher percentage of non-cycling cells among IGFBP-3-expressing cells than among controls. IGFBP-3 induction also resulted in morphological alterations, such as a flattened cytoplasm and increased granularity, suggesting that IGFBP-3 induces a senescence-like phenotype. The percentage of IGFBP-3 expressing cells with senescence-associated β-galactosidase activity was 3.4 times higher than control cells. Telomeric repeat amplification and real-time PCR showed that IGFBP-3 decreased telomerase activity by reducing the levels of the RNA component (hTR) and catalytic protein component with reverse transcriptase activity (hTERT) of telomerase in a dose-dependent manner. These results suggest that IGFBP-3 is a negative regulator of MCF-7 breast cancer cell growth by inducing senescence through telomerase suppression.
Anatomical damage caused by Bacillus thuringiensis variety israelensis in yellow fever mosquito Aedes aegypti (L.) larvae revealed by micro-computed tomography
Authors: Alba-Tercedor Javier , Vilchez Susana
Published: May 2023

With micro-computed tomography techniques, using the single-distance phase-retrieval algorithm phase contrast, we reconstructed enhanced rendered images of soft tissues of Aedes aeqypti fourth instar larvae after Bti treatment. In contrast to previous publications based on conventional microscopy, either optical or electron microscopy, which were limited to partial studies, mostly in the form of histological sections, here we show for the first time the effects of Bti on the complete internal anatomy of an insect. Using 3D rendered images it was possible to study the effect of the bacterium in tissues and organs, not only in sections but also as a whole. We compared the anatomy of healthy larvae with the changes undergone in larvae after being exposed to Bti (for 30 min, 1 h and 6 h) and observed the progressive damage that Bti produce. Damage to the midgut epithelia was confirmed, with progressive swelling of the enterocytes, thickening epithelia, increase of the vacuolar spaces and finally cell lysis, producing openings in the midgut walls. Simultaneously, the larvae altered their motility, making it difficult for them to rise to the surface and position the respiratory siphon properly to break surface tension and breathe. Internally, osmotic shock phenomena were observed, resulting in a deformation of the cross-section shape, producing the appearance of a wide internal space between the cuticle and the internal structures and a progressive collapse of the tracheal trunks. Taken together, these results indicate the death of the larvae, not by starvation as a consequence of the destruction of the epithelia of the digestive tract as previously stated, but due to a synergic catastrophic multifactor process in addition to asphyxia due to a lack of adequate gas exchange.
Experimental study on impermeability of Loess liner mixed with bentonite-HDTMA
Authors: Ming Zhang , Dongke Hu , Shaoyu Pan , Guozhou Chen
Published: May 2023

Permeability tests are performed by using the flexible wall permeameter to study the influence of bentonite-HDTMA (hexadecyl trimethyl ammonium bromide) on the permeability performance of Loess as a lining material in the solid waste landfill. Results show, the impermeability of compacted Loess in the middle and lower reaches of the Yellow River in China does not meet the standard requirement as the landfill liner. The permeability of Loess mixed with more than 10% bentonite ratio is less than 1.0 × 10−7 cm·s−1. The permeability of Loess increases slightly after mixing a small amount of HDTMA. The impermeability of Loess mixed with some bentonite-HDTMA ratio still meet the standard requirement. The HDTMA may destroy the soil aggregate structure and increase the soil permeate channel. The SEMs show that the bentonite clay particle can fill the pores between Loess coarse particles and improve the impermeability performance of the material. The digital photos show that HDTMA can effectively resist the development of soil macro-cracks induced by wetting–drying cycles, better for liner to maintain good impermeability. On this basis, the relationship between the hydraulic conductivity of bentonite-HDTMA modified Loess and dry density is constructed. From this study, Loess can be used as a lining material for landfills, when mixed with bentonite/HDTMA at ratios of 10%/0% or 14%/2%.
Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae
Authors: Fayet Maurine , Prybylski Nastasia , Collin Marie-Laure , Peyretaillade Eric , Wawrzyniak Ivan , Belkorchia Abdel , Akossi Reginald Florian , Diogon Marie , El Alaoui Hicham , Polonais Valérie , Delbac Frédéric
Published: May 2023

Microsporidia are obligate intracellular parasites able to infect a wide range of hosts from invertebrates to vertebrates. The success of their invasion process is based on an original organelle, the polar tube, which is suddenly extruded from the spore to inoculate the sporoplasm into the host cytoplasm. The polar tube is mainly composed of proteins named polar tube proteins (PTPs). A comparative analysis allowed us to identify genes coding for 5 PTPs (PTP1 to PTP5) in the genome of the microsporidian Anncaliia algerae. While PTP1 and PTP2 are found on the whole polar tube, PTP3 is present in a large part of the extruded polar tube except at its end-terminal part. On the contrary, PTP4 is specifically detected at the end-terminal part of the polar tube. To complete PTPs repertoire, sequential sporal protein extractions were done with high concentration of reducing agents. In addition, a method to purify polar tubes was developed. Mass spectrometry analysis conducted on both samples led to the identification of a PTP3-like protein (PTP3b), and a new PTP (PTP7) only found at the extremity of the polar tube. The specific localization of PTPs asks the question of their roles in cell invasion processes used by A. algerae.
Failure analysis and prediction of roof instability in end face under repeated mining using early warning system
Authors: Li Fei , Kong Dezhong , Li Qiang , Shang Yuqi , Cheng Zhanbo , He Liuquan
Published: May 2023

The overlying strata of the lower coal seam is easy to be collapsed causing the roof caving accident at the end face of the mining working face under repeated mining in close-distance coal seams. In order to predict the roof instability of the end face, the mechanical model of the granular arch structure is established in this study to further analyze its main influencing factors. The results show that the mining height of the working face, the advancing speed, the distance of coal seams, the tip-to-face distance, the strength of the surrounding rock and the support setting the load of the support are the main influencing factors on the roof caving of the end face. Subsequently, the prediction model of roof instability in the end face under repeated mining is constructed through the radial basis function neural network (RBFNN) and the above main influencing factors are regarded as input layer indexes. Meanwhile, the roof subsidence, coal wall deformation and support load are determined as the output layer indexes. The predicted results are closer to the results of sample tests. Finally, the early warning system, including monitoring and early warning, data query, emergency management, user management, and system settings, is designed to monitor roof conditions of the end face and timely warn the roof accidents. The field application proves that the system has good practical value, which is of great significance to intelligent prediction of coal mine stope disaster and prevent the end face roof disaster under repeated mining and. This will promote the safe and efficient construction of coal mine production.
Cracks propagation characteristics of double-hole delay blasting in soft-hard composite rock mass
Authors: Cui Jianbin , Xie Liangfu , Qin Yongjun , Liu Xuejun , Wang Jianhu , Qian Jiangu
Published: May 2023

By researching the distance between blasthole and interface of soft-hard rock strata, as well as the time of delay detonation, blasting effect of the rock mass will be more controllable. Firstly, validity of numerical method was authenticated from three angles: blasting coupled stress field, ratio of crushing zone radius to blasthole radius, and crack network state. Under the condition of soft-hard rock strata, numerical model of double-hole blasting was established by using PFC2D. Then delay blasting experiments were carried out under different relative positions of blasthole and interface. Ultimately, results were analyzed from three perspectives: crack network, crack quantity and rock fragment. Results show that: (1) When detonated in hard rock, if between interface and blasthole distance is greater than twice crushing zone radius, the closer blasthole is to the interface, the more obvious the “hook” phenomenon between the two blastholes is. With increasing delayed initiation time, “hook” phenomenon will weaken or even disappear. (2) Based on the crack information initiated in hard rock, the law of crack number varying with thickness of hard rock and delay time is obtained. (3) For initiation in hard rock, crack extension range is large, but less fragments are formed. The law is opposite to that initiation in structural plane and soft rock. Fragmentation area increases exponentially with increasing soft rock thickness, and exponential function is obtained.
Surveillance of prognostic risk factors in patients with SCCB using artificial intelligence: a retrospective study
Authors: Zhanghuang Chenghao , Zhang Zhaoxia , Wang Jinkui , Yao Zhigang , Ji Fengming , Wu Chengchuang , Ma Jing , Yang Zhen , Xie Yucheng , Tang Haoyu , Yan Bing
Published: May 2023

Small cell carcinoma of the bladder (SCCB) is a rare urological tumor. The prognosis of SCCB is abysmal. Therefore, this study aimed to construct nomograms that predict overall survival (OS) and cancer-specific survival (CSS) in SCCB patients. Information on patients diagnosed with SCCB during 2004–2018 was obtained from the Surveillance, Epidemiology, and End Results (SEER) database. Univariate and multivariate Cox regression models analyzed Independent risk factors affecting patients' OS and CSS. Nomograms predicting the OS and CSS were constructed based on the multivariate Cox regression model results. The calibration curve verified the accuracy and reliability of the nomograms, the concordance index (C-index), and the area under the curve (AUC). Decision curve analysis (DCA) assessed the potential clinical value. 975 patients were included in the training set (N = 687) and the validation set (N = 288). Multivariate COX regression models showed that age, marital status, AJCC stage, T stage, M stage, surgical approach, chemotherapy, tumor size, and lung metastasis were independent risk factors affecting the patients' OS. However, distant lymph node metastasis instead AJCC stage is the independent risk factor affecting the CSS in the patients. We successfully constructed nomograms that predict the OS and CSS for SCCB patients. The C index of the training set and the validation set of the OS were 0.747 (95% CI 0.725–0.769) and 0.765 (95% CI 0.736–0.794), respectively. The C index of the CSS were 0.749 (95% CI 0.710–0.773) and 0.786 (95% CI 0.755–0.817), respectively, indicating that the predictive models of the nomograms have excellent discriminative power. The calibration curve and the AUC also show good accuracy and discrimination of the nomograms. To sum up, We established nomograms to predict the OS and CSS of SCCB patients. The nomograms have undergone internal cross-validation and show good accuracy and reliability. The DCA shows that the nomograms have an excellent clinical value that can help doctors make clinical-assisted decision-making.
Privacy and the acceptance of centralized digital currencies in the U.S., India and Germany
Authors: Mehlkop Guido , Neumann Robert , von Hermanni Hagen
Published: May 2023

National governments around the world increasingly acknowledge the possibility of introducing new digital forms of money and implementing policies that trigger their adoption. Knowledge about the acceptance of such measures, however, is rather limited. Next to the regulatory uncertainty about the impact of a Central Bank Digital Currency (CBDC) on competition, on financial stability and questions on the integrity and technical implementations of a CBDC, recent announcements of the joint venture of e.g. the European Central Bank with a large and globally operating private company emphatically raise questions about data privacy. Therefore, we report results of a survey experiment conducted in the United States of America, Germany and India to investigate the acceptance of an app-based monthly digital payment similar to a Universal Basic Income and investigate its adoption across income levels. Controlling for privacy features and short-term vs. long-term incentives to adopt the digital payment app, we find strong reservations with regard to the involvement of multinational tech companies in establishing new digital mediums of exchange, while also finding contextual differences in acceptance levels between the studied populations.
Experimental study on damage characteristics of Beishan granite under single loading and multiple loading with AE techniques
Authors: Wang Zihui , Zhou Guanglei , Ge Xinbo
Published: May 2023

An insight into the damage characteristics of host rock is important for permanent disposal of high-level radioactive waste (HLW). Single loading and multiple loading tests for Beishan granite were carried out. The acoustic emission (AE) monitoring system was utilized simultaneously to measure the damage evolution in the specimens. The results show that, during single loading the AE signal has been maintained at a very high level after dilatancy. But a decreasing trend of AE signals can be observed when the axial stress approaches the peak stress. While during multiple loading, AE signals are mainly generated in the loading period in the elastic stage. However, in the failure and residual stages, AE signals generated in the unloading period are considerable. Most of the AE waveforms have a dominant frequency of less than 200 kHz and amplitude of less than 80 dB, and three frequency bands are obtained. In addition, the amplitude increases with the increase of loading stress. Furthermore, the obtained AE b-values indicate that the relative proportion of micro- to macro-cracks increases first and then decreases with increasing confining pressure. And the AE a-values and b-values confirms that micro cracks develop rapidly in the damage stage and lead to the formation of macro cracks in the residual stage for the specimens experienced multiple loading.
Plant viruses induce plant volatiles that are detected by aphid parasitoids
Authors: Milonas Panagiotis G. , Anastasaki Eirini , Psoma Aikaterini , Partsinevelos Georgios , Fragkopoulos Georgios N. , Kektsidou Oxana , Vassilakos Nikon , Kapranas Apostolos
Published: May 2023

Aphis gossypii (Sternorrhyncha: Aphididae) aphids are vectors of important plant viruses among which cucumber mosaic virus (CMV) and potato virus Y (PVY). Virus-infected plants attract aphid vectors and affect their behavior and growth performance either positively or negatively depending on mode of transmission. Viruses cause changes in the composition and the amount of volatile organic compounds (VOCs) released by the plant that attract aphids. The aphid parasitoid Aphidius colemani (Hymenoptera: Aphelinidae) has been shown to have higher parasitism and survival rates on aphids fed on virus-infected than aphids fed on non-infected plants. We hypothesized that parasitoids distinguish virus-infected plants and are attracted to them regardless of the presence of their aphid hosts. Herein, we examined the attraction of the A. colemani parasitoid to infected pepper plants with each of CMV or PVY without the presence of aphids. The dynamic headspace technique was used to collect VOCs from non-infected and CMV or PVY-infected pepper plants. Identification was performed with gas chromatography-mass spectrometry (GC–MS). The response of the parasitoids on virus-infected vs non-infected pepper plants was tested by Y-tube olfactometer assays. The results revealed that parasitoids displayed a preference to CMV and PVY infected plants compared to those that were not infected.
A longitudinal Q-study to assess changes in students’ perceptions at the time of pandemic
Authors: Akhtar-Danesh Noori , Brewer-Deluce Danielle , Saini Jessica , Wojkowski Sarah , Bayer Ilana , Saraco Anthony N. , Pitt Courtney , Wainman Bruce
Published: May 2023

The COVID-19 pandemic forced many universities and colleges to rapidly adopt online course delivery. As with any new foray, realizing the optimal aspects of a course to change became incredibly important for course instructors. In this study, we used a particularly sensitive method, i.e. Q-methodology, to evaluate changes based on students’ perceptions from fall 2020 to winter 2021. Q-methodology is commonly used to uncover shared values, opinions, and preferences. Using Q-methodology, students participating in both semesters of an undergraduate anatomy and physiology course were surveyed in fall 2020 and winter 2021. The Q-sample included 44 statements. Data from fall 2020 were treated as the baseline and changes in students’ perceptions from 2020 to 2021 were assessed. In total, 31 students completed both fall 2020 and winter 2021 course evaluations. Three salient factors emerged from the fall 2020 evaluation: Overtaxed students, Solo Achievers, and In-Person Learners. At the baseline, students were concerned mostly about the delivery of the course, then the winter 2021 evaluation showed how they were adjusting to online learning. The longitudinal Q-study proved to be robust in identifying changes in perceptions. These granular findings indicate how students might differ in viewing and evaluating online courses. This methodology can be used in redesigning and restructuring different components of an online course in higher education settings.
Diagnosis of sudden cardiac arrest using principal component analysis in automated external defibrillators
Authors: Pham Van-Su , Nguyen Anh , Dang Hoai Bac , Le Hai-Chau , Nguyen Minh Tuan
Published: May 2023

Sudden cardiac arrest (SCA) consisting of ventricular fibrillation and ventricular tachycardia considered as shockable rhythms is a life-threatening heart disease, which is treated efficiently by the automated external defibrillator (AED). This work proposes a novel design of the SAA, which includes a k-nearest neighbors model and a subset of 8 features extracted from the ECG segments, for the SCA diagnosis on the electrocardiogram (ECG) signal. These features are addressed as the most productive subset among 31 input features based on the evaluation of the feature correlation. The recursive feature elimination algorithm combined with the Boosting model and wise-patient fivefold cross-validation method is adopted for the calculation of the average feature importance, which shows the degree of feature correlation, to construct various input feature subsets. Moreover, component feature combinations known as the representatives of the input feature subsets with an enormous level of correlation and independence are transformed from the input subsets by the principal component analysis method. The wise-patient fivefold cross-validation procedure is used for the evaluation of these component feature combinations on the validation set. The proposed SAA is certainly efficient for SCA detection with a small number of the extracted feature and relatively high diagnosis performance such as accuracy of 99.52%, sensitivity of 97.69%, and specificity of 99.91%.