bluuebunny/crossref_metadata_2025 huggingface dataset:
Mass Spectrometry Metabolomics and Feature-Based Molecular Networking Reveals Population-Specific Chemistry in Some Species of the Sceletium Genus
Authors: Reddy Kaylan , Stander Marietjie A. , Stafford Gary I. , Makunga Nokwanda P.
Published: March 2022

The Sceletium genus has been of medicinal importance in southern Africa for millennia and Sceletium tortuosum (Aizoaceae), one of eight species in the genus has gained pharmaceutical importance as an anxiolytic and anti-depressant due to the presence of mesembrine alkaloids. S. tortuosum is used for the manufacture of herbal teas, dietary supplements and other phytopharmaceutical products. This study aimed to provide a metabolomic characterization of S. tortuosum and its sister species as these are not easy to distinguish using morphology alone. Plant samples were thus collected from various locations in the succulent Karoo (South Africa) and analyzed through liquid chromatography-mass spectrometry (LC-MS), using MSE fragmentation as a putative tool for chemical identities. Metabolomics-based analyses in combination with molecular networking were able to distinguish between the four species of Sceletium based on the presence of 4-(3,4-dimethyoxyphenyl)-4-[2-acetylmethlamino)ethyl]cyclohexanone (m/z 334.2020; RT 6.60 min), mesembrine (m/z 290.1757; RT 5.10 min) and 4'-O-demethylmesembrenol (m/z 276.1597; RT 4.17 min). Metabolomic profiles varied according to the different localities and metabolites occurred at variable quantitative levels in Sceletium ecotypes. Molecular networking provided the added advantage of being able to observe mesembrine alkaloid isomers and coeluting metabolites (from the joubertiamine group) that were difficult to discern without this application. By combining high-throughput metabolomics together with global and feature based-molecular networking, a powerful metabolite profiling platform that is able to discern chemical patterns within and between populations was established. These techniques were able to reveal chemotaxonomic relationships and allowed for the discovery of chemical markers that may be used as part of monitoring protocols during the manufacture of phytopharmaceutical and dietary products based on Sceletium.
Translation, Cultural Adaptation, Reliability, and Validity Testing of a Chinese Version of the Self-Administered Mediterranean Diet Scale
Authors: Li Jiajia , Ding Huirong , Wang Zheng , El-Ansary Doa , Adams Roger , Han Jia , Meng Shu
Published: March 2022

Mediterranean Diet management for people with cardiovascular disease (CVD) or CVD risk is supported by evidence. However, there is no valid Chinese language instrument for the measurement of adherence to this diet. The objective of this study was to generate a Chinese version of the Mediterranean Diet Scale (MDS-Chinese) and to validate a self-administered version with Chinese participants with CVD or CVD risk. The MDS-Chinese was created by translation and cultural adaptation and tested for psychometric properties. A panel of 10 experts in the field, who evaluated the MDS-Chinese content, showed that the content validity index ranged from 0.88 to 1.00. Sixteen native Chinese speakers with CVD or CVD risk evaluated the clarity of the MDS-Chinese, and the resulting instruction and items clarity scores ranged from 9.2 to 10.0. A total of 326 participants completed the MDS-Chinese and a Chinese version of the Coronary Artery Disease Education Questionnaire–Short Version (CADE-Q SV). Analysis indicated that the MDS-Chinese has 4 factors, and the Pearson's correlation between the MDS-Chinese and CADE-Q SV was 0.73. Fifty randomly selected participants completed the MDS-Chinese again with a 1-week interval to assess reliability. Internal consistency was acceptable (Cronbach's α was 0.62) and the inter-class correlation reliability coefficients (ICC) for each item ranged from 0.73 to 0.88. This study showed that the MDS-Chinese has acceptable reliability and validity for use among those in the Chinese population with CVD or CVD risk. Given that diet is one of the key secondary prevention strategies for management in cardiac rehabilitation, the MDS-Chinese instrument may be a useful and convenient tool for use with those in the Chinese population with CVD or with high risk of CVD, to monitor the level of Mediterranean diet (MD) adherence, information which is important for clinical practice. In addition, the establishment of the MDS-Chinese gives a fundamental tool for diet-related CVD research in the Chinese population. Moreover, employment of the MDS-Chinese in the Chinese community may improve awareness of the importance of a healthy diet in CVD prevention and management.

Clinical Trial Registration:http://www.chictr.org.cn/enIndex.aspx, identifier: ChiCTR2000032810.
Proteomic Analyses Identify Therapeutic Targets in Hepatocellular Carcinoma
Authors: Elmas Abdulkadir , Lujambio Amaia , Huang Kuan-lin
Published: March 2022

Hepatocellular carcinoma (HCC) is the fourth cause of cancer-related mortality worldwide. While many targeted therapies have been developed, the majority of HCC tumors do not harbor clinically actionable mutations. Protein-level aberrations, especially those not evident at the genomic level, present therapeutic opportunities but have rarely been systematically characterized in HCC. In this study, we performed proteogenomic analyses of 260 primary tumors from two HBV-related HCC patient cohorts with global mass-spectrometry (MS) proteomics data. Combining tumor-normal and inter-tumor analyses, we identified overexpressed targets including PDGFRB, FGFR4, ERBB2/3, CDK6 kinases and MFAP5, HMCN1, and Hsp proteins in HCC, many of which showed low frequencies of genomic and/or transcriptomic aberrations. Protein expression of FGFR4 kinase and Hsp proteins were significantly associated with response to their corresponding inhibitors. Our results provide a catalog of protein targets in HCC and demonstrate the potential of proteomics approaches in advancing precision medicine in cancer types lacking druggable mutations.
Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma
Authors: Huang Xinyang , Zhao Liangchao , Jin Yixun , Wang Zhuoxin , Li Tong , Xu Hui , Wang Qi , Wang Lifu
Published: March 2022

Background

Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant disease with a poor prognosis. More effective biomarkers and treatment options remain to be discovered. Mitotic Spindle Positioning (MISP), also called C19orf21, has been reported to be upregulated in several malignancies. However, the effects of MISP on PDAC have yet to be investigated.

Materials and Methods

The differential expression of MISP at the mRNA and protein levels were evaluated using Gene Expression Profiling Interactive Analysis 2 (GEPIA 2), Gene Expression Omnibus (GEO), and the Human Protein Atlas (HPA) databases, and was further verified by quantitative real-time PCR and western blotting in PDAC cell lines. Correlations between MISP expression and clinical characteristics were explored using Kaplan-Meier Plotter Database and clinical data from The Cancer Genome Atlas (TCGA). CCK-8 assays, Transwell assays, and immunoblotting were used to determine the role of MISP in PDAC proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in vitro. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were executed by the R package ‘clusterProfiler’. Correlations between MISP expression and immune cell infiltration, immune checkpoints, immunophenoscore (IPS) and the tumor mutational burden (TMB) in PDAC were explored using the R package ‘CIBERSORT’, the Tumor Immune Estimation Resource 2.0 (TIMER2.0), and The Cancer Immunome Atlas (TCIA) database based on TCGA data.

Result

MISP expression was significantly higher in pancreatic cancer tissues compared to normal pancreas tissues, which was associated with a poor prognosis. Increased expression of MISP was related to the proliferation, migration and invasion of PDAC cell lines. GO and KEGG pathway analyses determined that MISP is involved in the Ras signaling pathway and immune regulation. Higher expression of MISP was associated with decreased infiltration levels of activated CD4+ memory T cells, CD8+ T cells, M2 macrophages and neutrophils. Furthermore, increased MISP was associated with lower expression of immune checkpoint molecules, higher gene mutation burden and IPS.

Conclusions

This study reveals that MISP, which is associated with the progression and prognosis of PDAC, may exert a potential regulatory effect on immune infiltration and predict the response to immunotherapy in PDAC.
Role of CD155/TIGIT in Digestive Cancers: Promising Cancer Target for Immunotherapy
Authors: Wang Daijun , Gu Yanmei , Yan Xin , Huo Chengdong , Wang Guan , Zhao Yang , Teng Muzhou , Li Yumin
Published: March 2022

The tumor microenvironment restricts the function and survival of various immune cells by up-regulating inhibitory immune checkpoints, and participates in the immune escape of tumors. The development of immunotherapies targeting immune checkpoints, such as programmed cell death receptor 1 antibody and anti-cytotoxic T lymphocyte-associated antigen 4 antibody, has provided many options for cancer treatment. The efficacy of other immune checkpoint inhibitors is also under development and research. Among them, T cell immunoreceptor with Ig and ITIM domains (TIGIT) has shown excellent clinical application prospects. Correspondingly, poliovirus receptor (PVR, CD155), one of the main ligands of TIGIT, is mainly expressed in various human malignant tumors and myeloid cells. CD155 interacts with TIGIT on natural killer cells and T cells, mediating inhibitory immunomodulatory regulation. This study summarized the mechanism of CD155/TIGIT in regulating immune cells and its role in the occurrence and development of digestive system tumors, aiming to provide a new perspective for immunotherapy of digestive cancers.
The Inflammatory Status of Soluble Microenvironment Influences the Capacity of Melanoma Cells to Control T-Cell Responses
Authors: Bogéa Gabriela Muller Reche , Silva-Carvalho Amandda Évelin , Filiú-Braga Luma Dayane de Carvalho , Neves Francisco de Assis Rocha , Saldanha-Araujo Felipe
Published: March 2022

The development of immunotherapeutic approaches for the treatment of melanoma requires a better understanding of immunoescape mechanisms of tumor cells and how they interact with other tumor-resident cell types. Here, we evaluated how the conditioned media of resting (rCM) and immune-activated PBMCs (iCM) influence the ability of a metastatic melanoma cell line (MeWo) to control T-cells function. MeWo cells were expanded in RPMI, rCM, or iCM and the secretome generated after cell expansion was identified as MeSec (RPMI), niSec (non-inflammatory), or iSec (inflammatory secretome), respectively. Then, the immunomodulatory potential of such secretomes was tested in PHA-activated PBMCs. iCM induced higher levels of IFN-γ and IL-10 in treated melanoma cells compared to rCM, as well as higher IDO and PD-L1 expression. The iSec was able to inhibit T-cell activation and proliferation. Interestingly, PBMCs treated with iSec presented a reduced expression of the regulators of Th1 and Th2 responses T-BET and GATA-3, as well as low expression of IFN-γ, and co-stimulatory molecules TIM-3 and LAG-3. Importantly, our findings show that melanoma may benefit from an inflammatory microenvironment to enhance its ability to control the T-cell response. Interestingly, such an immunomodulatory effect involves the inhibition of the checkpoint molecules LAG-3 and TIM-3, which are currently investigated as important therapeutic targets for melanoma treatment. Further studies are needed to better understand how checkpoint molecules are modulated by paracrine and cell contact-dependent interaction between melanoma and immune cells. Such advances are fundamental for the development of new therapeutic approaches focused on melanoma immunotherapy.
Learning Methods During School Closure and Its Correlation With Anxiety and Health Behavior of Thai Students
Authors: Widyastari Dyah Anantalia , Kesaro Sarocha , Rasri Niramon , Saonuam Pairoj , Katewongsa Piyawat
Published: March 2022

Background

The sequential waves of epidemic spread of COVID-19 in Thailand have caused periodic closures of schools, and exposed students to different learning methods that require multiple adjustment strategies. This study aimed to examine how different learning methods may correlate with anxiety and health behavior (e.g., physical activity, active play, screen time, sleep) of primary and secondary school students in Thailand.

Methods

Thailand Report Card (TRC) Data (2021) was employed. The sample of the TRC was drawn by multi-stages random sampling stratified by region, district, urban/rural, school size, sex, and age to ensure national representativeness. A total of 6,078 Thai primary (64%) and secondary (36%) school students were included in the analysis.

Results

About two-thirds (66.4%) of the sample experienced a shift from traditional classroom to fully online learning, 6.9% experienced partial online instruction, 23.6% received handouts or written assignments, and 3.1% resumed traditional classroom learning. Compared to fully online learning, students who experienced traditional classroom (onsite) teaching were 37.8% less likely to report moderate-to severe anxiety (OR 0.6; p-value 0.021). There was no significant correlation between school closure-induced anxiety with overall physical activity (PA) and active play, but anxiety was significantly associated with screen time and sleep duration. Receiving handouts/written assignments only as the learning method was significantly correlated with PA, but two methods (handouts and onsite/traditional classroom) was significantly correlated with active play. Students who experienced classroom learning were also more likely to comply with recommended durations of screen time and sleep.

Conclusion

Although online learning was probably the most convenient choice during COVID-19 containment measures in Thailand, this method did not provide sufficient opportunity for PA and play. Online learning also encouraged an excessive use of screen media, and disrupted sleeping patterns. Online learning also pressured Thai students to make various adjustments in their daily routines that may have further aggravated anxiety.
The LINCE Project: A Pathway for Diagnosing NCL2 Disease
Authors: Rodrigues Daniel , de Castro Maria José , Crujeiras Pablo , Duat-Rodriguez Anna , Marco Ana Victoria , del Toro Mireia , Couce María L. , Colón Cristóbal
Published: March 2022

Introduction

Neuronal Ceroid Lipofuscinosis (NCL) comprises a clinically and genetically heterogeneous group of 13 neurodegenerative lysosomal storage disorders. Neuronal Ceroid lipofuscinosis type 2 disease (NCL2), caused by the deficient lysosomal enzyme tripeptidyl peptidase 1 (TPP1), is the only one with an approved enzyme replacement treatment (ERT). Early initiation of ERT appears to modify significantly the natural history of the disease. We aimed to shorten the time to diagnosis of NCL2.

Methods

In March 2017, we started per first time in Spain a selective screening program, the LINCE project, in pediatric patients with clinical symptoms compatible with NCL2 disease. The program covered the whole country. We distributed kits to pediatricians with the necessary material to assess patients. All samples in this study were received within one week of collection. Enzymatic activity determined on dried blood spots was the main method used to screen for TPP1 and palmitoyl protein thioesterase 1 (PPT1) for the differential diagnosis with neuronal ceroid lipofuscinosis type 1 (NCL1).

Results

Over a period of three years, we received 71 samples. The analysis was minimally invasive, relatively cheap and fast-executing. Three cases identified as a direct result of the selective screening strategy were confirmed by genetic study of NCL2 disease with a median age of 4.5 years. Our screening method has a specificity of 100%, and, with the absence to date of false negatives. We did not detect any NCL1-positive cases.

Conclusions

LINCE proved to be a simple, useful, and reliable tool for the diagnosis of NCL2, enabling clinicians to diagnose NCL2 faster. The presence of NCL2-positive cases in our population and availability of treatment may facilitate the inclusion of NCL2 in neonatal screening programs for early diagnosis.
In Vitro and In Vivo Studies for the Investigation of γ-Globin Gene Induction by Adhatoda vasica: A Pre-Clinical Study of HbF Inducers for β-Thalassemia
Authors: Iftikhar Fizza , Rahman Saeedur , Khan Muhammad Behroz Naeem , Khan Kanwal , Khan Muhammad Noman , Uddin Reaz , Musharraf Syed Ghulam
Published: March 2022

Fetal hemoglobin (HbF) is a potent genetic modifier, and the γ-globin gene induction has proven to be a sustainable therapeutic approach for the management of β-thalassemia. In this study, we have evaluated the HbF induction ability of A. vasica in vitro and in vivo, and the identification of potential therapeutic compounds through a bioassay-guided approach. In vitro benzidine-Hb assay demonstrated strong erythroid differentiation of K562 cells by A. vasica extracts. Subsequently, an in vivo study with an aqueous extract of A. vasica (100 mg/kg) showed significant induction of the γ-globin gene and HbF production. While in the acute study, the hematological and biochemical indices were found to be unaltered at the lower dose of A. vasica. Following the bioassay-guided approach, two isolated compounds, vasicinol (1) and vasicine (2) strongly enhanced HbF levels and showed prominent cellular growth kinetics with ample accumulation of total hemoglobin in K562 cultures. High HbF levels were examined by immunofluorescence and flow cytometry analysis, concomitant with the overexpression in the γ-globin gene level. Compound 1 (0.1 µM) and compound 2 (1 µM) resulted in a greater increase in F-cells (90 and 83%) with marked up (8-fold and 5.1-fold) expression of the γ-globin gene, respectively. Molecular docking studies indicated strong binding affinities of (1) and (2) with HDAC2 and KDM1 protein that predict the possible mechanism of compounds in inhibition of these epigenetic regulators in the γ-globin gene reactivation. Altogether, these observations demonstrated the therapeutic usefulness of A. vasica for fostering HbF production in clinical implications for blood disorders.
Research Progress on Natural Diterpenoids in Reversing Multidrug Resistance
Authors: Deng Zhuo-fen , Bakunina Irina , Yu Hua , Han Jaehong , Dömling Alexander , Ferreira Maria-José U , Zhang Jian-ye
Published: March 2022

Multidrug resistance (MDR) is one of the main impediments in successful chemotherapy in cancer treatment. Overexpression of ATP-binding cassette (ABC) transporter proteins is one of the most important mechanisms of MDR. Natural products have their unique advantages in reversing MDR, among which diterpenoids have attracted great attention of the researchers around the world. This review article summarizes and discusses the research progress on diterpenoids in reversing MDR.
Hypothermia Induced Impairment of Platelets: Assessment With Multiplate vs. ROTEM—An In Vitro Study
Authors: Wallner Bernd , Schenk Bettina , Paal Peter , Falk Markus , Strapazzon Giacomo , Martini Wenjun Z. , Brugger Hermann , Fries Dietmar
Published: March 2022

Introduction: This experimental in vitro study aimed to identify and characterize hypothermia-associated coagulopathy and to compare changes in mild to severe hypothermia with the quantitative measurement of rotational thromboelastometry (ROTEM) and multiple-electrode aggregometry (MULTIPLATE).

Methods: Whole blood samples from 18 healthy volunteers were analyzed at the target temperatures of 37, 32, 24, 18, and 13.7°C with ROTEM (ExTEM, InTEM and FibTEM) and MULTIPLATE using the arachidonic acid 0.5 mM (ASPI), thrombin receptor-activating peptide-6 32 µM (TRAP) and adenosine diphosphate 6.4 µM (ADP) tests at the corresponding incubating temperatures for coagulation assessment.

Results: Compared to baseline (37°C) values ROTEM measurements of clotting time (CT) was prolonged by 98% (at 18°C), clot formation time (CFT) was prolonged by 205% and the alpha angle dropped to 76% at 13.7°C (p < 0.001). At 24.0°C CT was prolonged by 56% and CFT by 53%. Maximum clot firmness was only slightly reduced by ≤2% at 13.7°C. Platelet function measured by MULTIPLATE was reduced with decreasing temperature (p < 0.001): AUC at 13.7°C −96% (ADP), −92% (ASPI) and −91% (TRAP).

Conclusion: Hypothermia impairs coagulation by prolonging coagulation clotting time and by decreasing the velocity of clot formation in ROTEM measurements. MULTIPLATE testing confirms a linear decrease in platelet function with decreasing temperatures, but ROTEM fails to adequately detect hypothermia induced impairment of platelets.
Wheat Quality Formation and Its Regulatory Mechanism
Authors: Peng Yanchun , Zhao Yun , Yu Zitong , Zeng Jianbin , Xu Dengan , Dong Jing , Ma Wujun
Published: March 2022

Elucidation of the composition, functional characteristics, and formation mechanism of wheat quality is critical for the sustainable development of wheat industry. It is well documented that wheat processing quality is largely determined by its seed storage proteins including glutenins and gliadins, which confer wheat dough with unique rheological properties, making it possible to produce a series of foods for human consumption. The proportion of different gluten components has become an important target for wheat quality improvement. In many cases, the processing quality of wheat is closely associated with the nutritional value and healthy effect of the end-products. The components of wheat seed storage proteins can greatly influence wheat quality and some can even cause intestinal inflammatory diseases or allergy in humans. Genetic and environmental factors have great impacts on seed storage protein synthesis and accumulation, and fertilization and irrigation strategies also greatly affect the seed storage protein content and composition, which together determine the final end-use quality of wheat. This review summarizes the recent progress in research on the composition, function, biosynthesis, and regulatory mechanism of wheat storage proteins and their impacts on wheat end-product quality.
The Regulation of Pea (Pisum sativum L.) Symbiotic Nodule Infection and Defense Responses by Glutathione, Homoglutathione, and Their Ratio
Authors: Ivanova Kira A. , Chernova Ekaterina N. , Kulaeva Olga A. , Tsyganova Anna V. , Kusakin Pyotr G. , Russkikh Iana V. , Tikhonovich Igor A. , Tsyganov Viktor E.
Published: March 2022

In this study, the roles of glutathione (GSH), homoglutathione (hGSH), and their ratio in symbiotic nodule development and functioning, as well as in defense responses accompanying ineffective nodulation in pea (Pisum sativum) were investigated. The expression of genes involved in (h)GSH biosynthesis, thiol content, and localization of the reduced form of GSH were analyzed in nodules of wild-type pea plants and mutants sym33-3 (weak allele, “locked” infection threads, occasional bacterial release, and defense reactions) and sym33-2 (strong allele, “locked” infection threads, defense reactions), and sym40-1 (abnormal bacteroids, oxidative stress, early senescence, and defense reactions). The effects of (h)GSH depletion and GSH treatment on nodule number and development were also examined. The GSH:hGSH ratio was found to be higher in nodules than in uninoculated roots in all genotypes analyzed, with the highest value being detected in wild-type nodules. Moreover, it was demonstrated, that a hGSHS-to-GSHS switch in gene expression in nodule tissue occurs only after bacterial release and leads to an increase in the GSH:hGSH ratio. Ineffective nodules showed variable GSH:hGSH ratios that correlated with the stage of nodule development. Changes in the levels of both thiols led to the activation of defense responses in nodules. The application of a (h)GSH biosynthesis inhibitor disrupted the nitrogen fixation zone in wild-type nodules, affected symbiosome formation in sym40-1 mutant nodules, and meristem functioning and infection thread growth in sym33-3 mutant nodules. An increase in the levels of both thiols following GSH treatment promoted both infection and extension of defense responses in sym33-3 nodules, whereas a similar increase in sym40-1 nodules led to the formation of infected cells resembling wild-type nitrogen-fixing cells and the disappearance of an early senescence zone in the base of the nodule. Meanwhile, an increase in hGSH levels in sym40-1 nodules resulting from GSH treatment manifested as a restriction of infection similar to that seen in untreated sym33-3 nodules. These findings indicated that a certain level of thiols is required for proper symbiotic nitrogen fixation and that changes in thiol content or the GSH:hGSH ratio are associated with different abnormalities and defense responses.
Spatial Regression Models for Field Trials: A Comparative Study and New Ideas
Authors: Hawinkel Stijn , De Meyer Sam , Maere Steven
Published: March 2022

Naturally occurring variability within a study region harbors valuable information on relationships between biological variables. Yet, spatial patterns within these study areas, e.g., in field trials, violate the assumption of independence of observations, setting particular challenges in terms of hypothesis testing, parameter estimation, feature selection, and model evaluation. We evaluate a number of spatial regression methods in a simulation study, including more realistic spatial effects than employed so far. Based on our results, we recommend generalized least squares (GLS) estimation for experimental as well as for observational setups and demonstrate how it can be incorporated into popular regression models for high-dimensional data such as regularized least squares. This new method is available in the BioConductor R-package pengls. Inclusion of a spatial error structure improves parameter estimation and predictive model performance in low-dimensional settings and also improves feature selection in high-dimensional settings by reducing “red-shift”: the preferential selection of features with spatial structure. In addition, we argue that the absence of spatial autocorrelation (SAC) in the model residuals should not be taken as a sign of a good fit, since it may result from overfitting the spatial trend. Finally, we confirm our findings in a case study on the prediction of winter wheat yield based on multispectral measurements.
Genome-Wide Association Study of Soybean Germplasm Derived From Canadian × Chinese Crosses to Mine for Novel Alleles to Improve Seed Yield and Seed Quality Traits
Authors: Priyanatha Chanditha , Torkamaneh Davoud , Rajcan Istvan
Published: March 2022

Genome-wide association study (GWAS) has emerged in the past decade as a viable tool for identifying beneficial alleles from a genomic diversity panel. In an ongoing effort to improve soybean [Glycine max (L.) Merr.], which is the third largest field crop in Canada, a GWAS was conducted to identify novel alleles underlying seed yield and seed quality and agronomic traits. The genomic panel consisted of 200 genotypes including lines derived from several generations of bi-parental crosses between modern Canadian × Chinese cultivars (CD-CH). The genomic diversity panel was field evaluated at two field locations in Ontario in 2019 and 2020. Genotyping-by-sequencing (GBS) was conducted and yielded almost 32 K high-quality SNPs. GWAS was conducted using Fixed and random model Circulating Probability Unification (FarmCPU) model on the following traits: seed yield, seed protein concentration, seed oil concentration, plant height, 100 seed weight, days to maturity, and lodging score that allowed to identify five QTL regions controlling seed yield and seed oil and protein content. A candidate gene search identified a putative gene for each of the three traits. The results of this GWAS study provide insight into potentially valuable genetic resources residing in Chinese modern cultivars that breeders may use to further improve soybean seed yield and seed quality traits.
A Study of Family Process Factors of Social Anxiety on the Internet Based on Big Data—Take Guangxi University Students as an Example
Authors: Bi Dexu
Published: March 2022

Background

Along with the popularization of the new medium of interpersonal communication, many researchers have found that the use of social media has brought about many mental health problems. For example, the virtual nature, vulnerability, and uncertainty of online communication lead to reduced online trust, causing interaction anxiety (IA). The data footprints left on the Internet are processed by malicious elements for big data, leading to the leakage of personal privacy data, bringing content sharing anxiety (SAC) and privacy concern anxiety (PAC), which are all typical forms of online social anxiety. In the face of this situation, analyzing the influence of online social networking on the social psychology of university students and guiding it has become an inevitable issue in the Internet era.

Methods

Learning from the classification of family environment, a self-administered family process factor questionnaire and the Social Anxiety Scale for Social Media Users (SAS-SMU) were used to investigate the online social anxiety of Guangxi University students. The study used SPSS26.0 and Stata for data analysis and descriptive statistics, ANOVA, t-test, and linear regression analysis were used to explore the relationship between family process factors and online social anxiety of the university students.

Results

The results showed that except for parental supervision (p > 0.05), the effects of interparental relationship, parent-child relationship, sibling relationship, and family atmosphere on university students' online social anxiety were statistically significant and showed positive correlations (F/t = 6.64, 3.53, 4.15, 5.94; p < 0.05). Multiple linear regression analysis showed that university students' total online social anxiety score = 36.914−4.09 × good parental relationship−4.16 × good family atmosphere−3.42 × good sibling relationship.

Conclusions

Based on the family systems theory, it is suggested that a comprehensive intervention should be conducted for the coupled system (parental relationship) and sibling system (non-only child's sibling relationship) in the family and focus on the protective factors of parental harmony, sibling relationship harmony, and relaxed family atmosphere. In the specific implementation method, the collaborative shared healthcare plan (CSHCP) can be used to strengthen remote family emotional interaction and avoid Internet addiction. For university students with online social anxiety disorders, their personal health records (PHRs) can be maintained permanently and safely using the Star File System (IPFS), in addition to the convenience of IPFS data extraction, which is more conducive to the timely and long-term tracking treatment of anxious university students.
When Dancers Are Butterflies: How the Brain Understands Metaphors
Authors: Kalandadze Tamara , Tonini Elisabetta , Bambini Valentina
Published: March 2022

When we speak, we often say things that are different from what we actually mean. For example, at a ballet, we might say that the dancers are butterflies. Dancers are not really butterflies, but this statement is not a lie; it is a metaphor. A metaphor is a figure of speech quite common in our everyday language and in books. In this article, we explain what metaphors are, why we use them, and what happens in our brains when we must understand a metaphor. Understanding metaphors requires a lot of brain work, which is why it is not always easy. Some people might find metaphors particularly difficult to understand, for example, very young children and people who are autistic. At the end of the article, we suggest things that teachers and speech and language therapists can do to help those who have difficulty understanding metaphors.
Multi-Physics Constraints at Different Densities to Probe Nuclear Symmetry Energy in Hyperonic Neutron Stars
Authors: Ghosh Suprovo , Pradhan Bikram Keshari , Chatterjee Debarati , Schaffner-Bielich Jürgen
Published: March 2022

The appearance of strangeness in the form of hyperons within the inner core of neutron stars is expected to affect its detectable properties, such as its global structure or gravitational wave emission. This work explores the parameter space of hyperonic stars within the framework of the Relativistic Mean Field model allowed by the present uncertainties in the state-of-the-art nuclear and hypernuclear experimental data. We impose multi-physics constraints at different density regimes to restrict the parameter space: Chiral effective field theory, heavy-ion collision data, and multi-messenger astrophysical observations of neutron stars. We investigate possible correlations between empirical nuclear and hypernuclear parameters, particularly the symmetry energy and its slope, with observable properties of neutron stars. We do not find a correlation for the hyperon parameters and the astrophysical data. However, the inclusion of hyperons generates a tension between the astrophysical and heavy-ion data constraining considerably the available parameter space.
Waves of Extremism: An Applied Ethnographic Analysis of the Bosnia and Herzegovina Football Terraces
Authors: Testa Alberto
Published: March 2022

This article offers an overview of a four-month research project, conducted in 2019/2020, which studied extremism in the Bosnia and Herzegovina (BiH) football terraces. This work was funded by the International Organisation for Migration - United Nations and by the United States Agency for International Development (USAID). The research focused on risk factors and how these may govern the “entry” of BiH youth into extreme hard-core football fans groups (Ultras1) and prolong their involvement in them. The study highlighted the nature of these groups and their activity providing detailed recommendations for BiH policymakers, security agencies, and football federations and clubs who wish to understand and effectively respond to this emergent threat for public security in BiH.
Anthropometrics, Athletic Abilities and Perceptual-Cognitive Skills Associated With Baseball Pitching Velocity in Young Athletes Aged Between 10 and 22 Years Old
Authors: Tremblay Mathieu , Tétreau Charles , Corbin-Berrigan Laurie-Ann , Descarreaux Martin
Published: March 2022

Objective assessments of players performance and individual characteristics are increasingly used in baseball. However, evidence linking individual characteristics to players' performance are scarce. The purpose of the study was to identify across ages, in younger males and females, and to compare, in younger males, the anthropometrics, athletic abilities and perceptual-cognitive skills associated with baseball pitcher's ball velocity. A cross-sectional design was used to conduct this study. Male and female athletes completed a sociodemographic questionnaire followed by anthropometric, athletic ability, perceptual-cognitive skill and pitching velocity assessments. Athletes were categorized by their age categories (11U, 13U, 15U, 18U, 21U). To evaluate the athletes' anthropometrics, height and weight, BMI, waist circumference, arms segmental length and girth were measured. Athletic abilities were assessed using athletes' grip strength, upper body power, vertical jump height, sprint, change of direction, and dynamic balance. Perceptual-cognitive skills performance was assessed with the Neurotracker platform. Pitching performance assessment was completed using the athletes' average fastball velocity. Kendall Tau's correlation coefficient was used to assess relationships between variables and pitching velocity in male athletes (p < 0.05). A 1-way ANOVA was performed to identify differences between age categories for all variables in male athletes (p < 0.05). In male athletes, without age categories discrimination, all anthropometric, athletic ability and perceptual-cognitive skill factors were associated with pitching velocity with associations ranging from τ = 0.185 for perceptual-cognitive skills to τ = 0.653 for left arm grip strength. The results showed that significant differences exist between age categories for anthropometric, athletic ability and perceptual-cognitive skill assessments. The study showed that associations between anthropometrics and pitching velocity, and athletic abilities and pitching velocity vary across age categories. Descriptive data of female athletes results regarding anthropometrics, athletic abilities, perceptual-cognitive skills and pitching velocity are also presented. Gender differences should be investigated in future studies exploring baseball pitching performance.
Toward a Sustainable Food System in West Papua, Indonesia: Exploring the Links Between Dietary Transition, Food Security, and Forests
Authors: Nurhasan Mulia , Maulana Agus Muhamad , Ariesta Desy Leo , Usfar Avita Aliza , Napitupulu Lucentezza , Rouw Aser , Hurulean Ferdinandus , Hapsari Azizah , Heatubun Charlie D. , Ickowitz Amy
Published: March 2022

Natural tropical forests cover 89% of the land area of West Papua Province, Indonesia. Forests have traditionally been an important part of local food systems for Indigenous Papuans. Despite the contribution of forests to food security, West Papua has been ranked as one of the most food-insecure provinces in Indonesia, with high rates of both under-and-overnutrition. This paper aims to discuss the dietary transition taking place in West Papua and uses local perspectives to explore the link between changes in diets, food security, and forests. We used mixed methods with a triangulation design to corroborate the quantitative data that we present from two rounds of the National Socioeconomic Survey (SUSENAS) on food consumption for West Papua from 2008 and 2017, with information from four focus group discussions with institutional and local stakeholders. The quantitative analysis showed that West Papua is experiencing a dietary transition, moving away from the consumption of traditional foods, such as sago, tubers, wild meat, and fresh legumes, toward diets with more rice, chicken, tofu, and tempeh. The consumption of processed and ultra-processed food (UPF) has increased while the consumption of fresh food has decreased. The qualitative analysis confirmed these findings. The institutional stakeholders expressed a desire for Papuans to return to eating traditional diets for better food security, whereas the local stakeholders worried about their children's high consumption of UPFs. We also found a disconnect between how food security is measured by the national Food Security Index (FSI) and the point of view of the institutional stakeholders. While the FSI indicators are more infrastructure-related measures, the institutional stakeholders link food security with the availability, accessibility, stability, and sustainability of the food sources in their surrounding environment, especially the forests. The institutional stakeholders support the commitment of the provincial government to maintain at least 70% of the forest cover in West Papua, as stated in the Manokwari Declaration although they expressed the need for more clarity on how this will impact their food security. The Indonesian government and the international community should support this initiative and carry it out with substantial input from local Papuan stakeholders.
The Effect of Harvest Date on the Chemical Composition and Fodder Yield of Guizotia abyssinica (Guizotia abyssinica (L.f.) Cass.) under the Climatic Conditions of South-West Poland
Authors: Szuba-Trznadel Anna , Hikawczuk Tomasz , Jama-Rodzeńska Anna , Król Zygmunt , Fuchs Bogusław
Published: March 2022

Guizotia abyssinica (Guizotia abyssinica (L.f.) Cass.) is regarded as a minor oilseed crop, which is important in terms of its nutritional value and potential uses. An important benefit of this crop is its cultivation for green matter and seed yield even under the difficult conditions of marginal cultivation. Guizotia abyssinica has not yet gained popularity in Europe. However, in a changing climate and taking into account its feeding value, it could gain in importance for ruminant nutrition in the near future. A field experiment was conducted in 2018 and 2019 in the west part of Poland on a farm in Opolskie voivodship (commune Prudnik). The objective of the field study was to assess the effect of three harvest dates on the yield and the chemical composition of fodder in a 90-day cultivation cycle in each year of the study. Stage of growth seems to be the most important factor affecting yield and the chemical composition of Guizotia abyssinica. In both years of the research, the green fodder yield reached the highest value after 90 days from sowing—32.5 and 36.9 t ha−1—while dry matter was 6.85 and 7.99 t ha−1, respectively. Chemical composition was also significantly dependent on harvesting date. Crude protein (in 2018 from 154.5 to 100.7 g kg−1 and in 2019 from 148.3 to 78.2 g kg−1) and crude ash (in 2018 from 165.8 to 98.7 and in 2019 from 155.2 to 89.9 g kg−1) content decreased with progressive harvest date while nitrogen-free extracts (in 2018 from 500.5 to 562.0 g kg−1 and in 2019 from 582.2 to 605.2 g kg−1) and gross energy increased (in 2018 from 19.4 to 21.17 MJ kg−1 and in 2019 from 18.63 to 19.73 MJ kg−1.). The most favorable date for harvesting green forage is 90 days after sowing, due to the significantly highest yield of fresh and dry matter of green forage and with potential as a forage for animals.
Genome-Wide Association Studies of Plant Architecture-Related Traits in the Chinese Soybean Mini Core Collection
Authors: Ding Wentao , Zhang Xiaoli , Liu Dandan , Li Chen , Wang Congcong , Sun Ruidong , Jin Xiangpei , Guo Na , Zhao Jinming , Xing Han
Published: March 2022

Plant architecture traits are closely related to plant biomass, lodging, and photosynthetic efficiency, which in turn affect soybean yield. In this study, we investigated a Chinese soybean mini core collection consisting of 224 germplasm accessions for four plant architecture-related traits (plant height (PH), number of nodes on main stem (NN), branch number (BN), and stem diameter (DI)) under three environments and conducted a genome-wide association study (GWAS) based on 1514 single nucleotide polymorphisms (SNPs). A total of 41 SNPs were found to be significantly associated with PH, NN, BN, and DI in two or more environments. Among these SNPs, 15 were located in regions in which plant architecture-related QTLs had been reported in previous studies, and 26 were new genetic loci. In addition, 18 potential candidate genes for plant architecture-related traits were obtained by predicting the genes in the interval of four large-effect markers (BARC-017097-02199, Map-2213, BARC-014639-01604, and Map-2223). This research will help to illuminate the genetic basis of soybean plant architecture-related traits and accelerate the process of plant architecture breeding by molecular marker-assisted selection in soybean.
Water Accounting and Productivity Analysis to Improve Water Savings of Nile River Basin, East Africa: From Accountability to Sustainability
Authors: Hirwa Hubert , Zhang Qiuying , Li Fadong , Qiao Yunfeng , Measho Simon , Muhirwa Fabien , Xu Ning , Tian Chao , Cheng Hefa , Chen Gang , Ngwijabagabo Hyacinthe , Turyasingura Benson , Itangishaka Auguste Cesar
Published: March 2022

Complete water accounting (WA) and crop water productivity (CWP) analysis is crucial for evaluating water use efficiency (WUE). This study aims to evaluate the contributions of hydro-meteorological factors to the changes of WA and CWP and subsequent WUE based on the data from 2009–2020 in the Nile River Basin (NRB), East Africa (EA). The Mann-Kendall (MK) statistical test and Sen’s slope estimator were applied to detect the trends of climatic factors, and the AquaCrop model was used to simulate the crop yields in response to water balance and consumption based on crop physiological, soil water, and salt budget concepts. For the years 2012 and 2019, the mean of climatic water deficit P − ETa was 71.03 km3 and 37.03 km3, respectively, which was expected to rise to ~494.57 km3 by 2050. The results indicated that the basin water budget was unbalanced due to the coupled impact of year-to-year hot and dry conditions and increase in water abstraction, an indication of water deficit or stress. CWP and WUE increased during the study period with different changing patterns. CWP was also found to correlate to the yield of major crops (p-value > 0.05). It was concluded that climatic factors influenced the crop yield, CWP, and WUE in the study area. Thus, the improvement of CWP and WUE should rely on advanced water-saving innovations. The findings of this study could help water managers to improve water productivity by focusing on water account potentials and creating regional advantages by deploying water in combination with surplus flow from upstream to downstream consumption.
Authenticity Identification of Saccharum officinarum and Saccharum spontaneum Germplasm Materials
Authors: Li Xueting , Guo Yirong , Huang Fei , Wang Qiusong , Chai Jin , Yu Fan , Wu Jiayun , Zhang Muqing , Deng Zuhu
Published: March 2022

Sugarcane is an important sugar and energy crop in the world. Germplasm innovation is a significant way to breed breakthrough sugarcane varieties. Modern sugarcane varieties all contain the blood relationship of Saccharum officinarum and Saccharum spontaneum. High sugar results from S. officinarum and the resistance genes from S. spontaneum. In order to improve the sugarcane quality, breeders use S. officinarum and S. spontaneum to cross and obtain hybrid offspring with high sugar and high resistance. Therefore, the authenticity of S. officinarum and S. spontaneum progeny materials directly affects the efficiency of sugarcane breeding. In this study, the tetra-primer amplification hindered mutation system (ARMS PCR) was used to identify ten suspected S. officinarum and eleven suspected S. spontaneum germplasm materials, then further validated by chromosome counting and genome in situ hybridization (GISH). Among the ten suspected S. officinarum materials to be identified, three were real S. officinarum materials, they were 14NG124, 51NG103, and Guan A. Nine of the eleven suspected S. spontaneum to be identified were fake S. spontaneum materials, these were Yunge 2007-12-165, Guangxi 87-20, Yunnan 82-16, Yunge 2007-11, YNLC 16, Laos No. 2, Yunnan 82-29, 2015-83, and 2013-20. The ARMS PCR results were the same as the GISH results. The three real S. officinarum materials had 80 chromosomes. Using ARMS PCR and GISH, three S. officinarum and nine S. spontaneum materials were proven to be authentic. Through chromosome number statistics, it was found that the three real S. officinarum had 80 chromosomes. Authentic materials were identified and selected to enrich the genetic background of sugarcane through hybridization and reduce the influence on the breeding process of the misuse of fake S. officinarum and S. spontaneum.