bluuebunny/crossref_metadata_2025 huggingface dataset:
Human 5’-tailed Mirtrons are Processed by RNaseP
Authors: Zia M. Farid , Peter Jacob , Hoover Johnathan , Chen Kuan-hui E. , Flynt Alex
Published: October 2021

Approximately a thousand microRNAs (miRNAs) are documented from human cells. A third appear to transit non-canonical pathways that typically bypass processing by Drosha, the dedicated nuclear miRNA producing enzyme. The largest class of non-canonical miRNAs are mirtrons which eschew Drosha to mature through spliceosome activity. While mirtrons are found in several configurations, the vast majority of human mirtron species are 5’-tailed. For these mirtrons, a 3’ splice site defines the 3’ end of their hairpin precursor while a “tail” of variable length separates the 5’ base of the hairpin from the nearest splice site. How this tail is removed is not understood. Here we examine sequence motifs in 5’-tailed mirtrons and interactions with RNA turnover processes to characterize biogenesis processes. Through studying the high confidence 5’-tailed mirtron, hsa-miR-5010, we identify RNaseP as necessary and sufficient for “severing” the 5’ tail of this mirtron. Further, depletion of RNaseP activity globally decreased 5’-tailed mirtron expression implicating this endoribonuclease in biogenesis of the entire class. Moreover, as 5’-tailed mirtron biogenesis appears to be connected to tRNA processing we found a strong correlation between accumulation of tRNA fragments (tRFs) and 5’-tailed mirtron abundance. This suggests that dysregulation of tRNA processing seen in cancers may also impact expression of the ∼400 5’-tailed mirtrons encoded in the human genome.

Abundant non-canonical human miRNAs referred to as tailed mirtrons are processed by RNaseP, which “severs” tail nucleotides to yield a precursor hairpin suitable for Dicer processing. Biogenesis of these miRNAs is correlated with tRFs, which are also products of RNaseP processing.
Integrating Subclonal Response Heterogeneity to Define Cancer Organoid Therapeutic Sensitivity
Authors: Kratz Jeremy D. , Rehman Shujah , Johnson Katherine A. , Gillette Amani A. , Sunil Aishwarya , Favreau Peter F. , Pasch Cheri A. , Miller Devon , Zarling Lucas C. , Yeung Austin H. , Clipson Linda , Anderson Samantha J. , DeZeeuw Alyssa K. , Sprackling Carley M. , Lemmon Kayla K. , Abbott Daniel E. , Burkard Mark E. , Bassetti Michael F. , Eickhoff Jens C. , Foley Eugene F. , Heise Charles P. , Kimple Randall J. , Lawson Elise H. , LoConte Noelle K. , Lubner Sam J. , Mulkerin Daniel L. , Matkowskyj Kristina A. , Sanger Cristina B. , Uboha Nataliya V. , Mcilwain Sean J. , Ong Irene M. , Carchman Evie H. , Skala Melissa C. , Deming Dustin A.
Published: October 2021

Tumor heterogeneity is predicted to confer inferior clinical outcomes, however modeling heterogeneity in a manner that still represents the tumor of origin remains a formidable challenge. Sequencing technologies are limited in their ability to identify rare subclonal populations and predict response to the multitude of available treatments for patients. Patient-derived organotypic cultures have significantly improved the modeling of cancer biology by faithfully representing the molecular features of primary malignant tissues. Patient-derived cancer organoid (PCO) cultures contain numerous individual organoids with the potential to recapitulate heterogeneity, though PCOs are most commonly studied in bulk ignoring any diversity in the molecular profile or treatment response. Here we demonstrate the advantage of evaluating individual PCOs in conjunction with cellular level optical metabolic imaging to characterize the largely ignored heterogeneity within these cultures to predict clinical therapeutic response, identify subclonal populations, and determine patient specific mechanisms of resistance.
Post-injury born oligodendrocytes integrate into the glial scar and inhibit growth of regenerating axons by premature myelination
Authors: Xing Jian , Rheaume Bruce A. , Kim Juhwan , Lukomska Agnieszka , Sajid Muhammad S. , Damania Ashiti , Trakhtenberg Ephraim F.
Published: October 2021

Pathologies of the central nervous system (CNS) white matter often result in permanent functional deficits because mature mammalian projection neurons fail to regenerate long-distance axons after injury. A major barrier to axonal regenerative research is that the CNS axons that regenerate in response to experimental treatments stall growth before reaching their post-synaptic targets. Here, we test the hypothesis that premature, de novo, myelination of regenerating axons stalls their growth, even after bypassing the glial scar. To test this hypothesis, first, we used single cell RNA-seq (scRNA-seq) and immunohistological analysis to investigate whether post-injury born oligodendrocytes integrate into the glial scar after optic nerve injury. Then, we used a multiple sclerosis model of demyelination concurrently with the stimulation of axon regeneration by Pten knockdown (KD) in projection neurons after optic nerve injury. We found that post-injury born oligodendrocytes integrate into the glial scar, where they are susceptible to the demyelination treatment, which prevented premature myelination, and thereby enhanced Pten KD-stimulated axon regeneration. We also present a website for comparing the gene expression of scRNA-seq-profiled optic nerve oligodendrocytes under physiological and pathophysiological conditions.

SIGNIFICANCE STATEMENT

Myelin debris from degenerating axons along with reactive astrocytes in the glial scar inhibit CNS axon regeneration. However, even with the recently developed experimental approaches which activate axons to regenerate passed the glial scar, almost all axons still stall growth before reaching their post-synaptic targets. Here, we show that post-injury born oligodendrocytes integrate into the glial scar, and that other than myelin debris, live oligodendrocytes prematurely myelinating the regenerating axons inhibit growth, even if the axons have already regenerated passed the glial scar.
Maf is a regulator of differentiation for gut immune epithelial cell Microfold cell (M cell)
Authors: George Joel Johnson , Arrojo Martins Fábio Tadeu , Martin-Diaz Laura , Viiri Keijo
Published: October 2021

Microfold cells (M cells) are a specialized subset of epithelial intestinal cells responsible for immunosurveillance of the gastrointestinal tract. M cells are located in the Peyer’s patches and are crucial for monitoring and the transcytosis of antigens, microorganisms, and pathogens via their mature receptor GP2. A mature M cell with Gp2 receptor aids in the uptake of antigens, which are passed through the single layer of epithelium and presented to underlying antigen-presenting cells and processed further down-stream with B cells, T cells, and dendritic cells. Recent studies revealed several transcription factors and ligands responsible for the development and differentiation of mature M cells however, an exhaustive list of factors remains to be elucidated. Our recent work on the epigenetic regulation of M cell development found 12 critical transcription factors that were controlled by the polycomb recessive complex 2. Musculoaponeurotic fibrosarcoma transcription factor (Maf) was identified as a gene regulated by the polycomb repressive complex (PRC2) during the development of M cells. In this paper, we explore Maf’s critical role in M cell differentiation and maturation. Maf falls under the purview of RANKL signaling, is localized in the Peyer’s patches of the intestine, and is expressed by M cells. Given that, complete knockout of the Maf gene leads to a lethal phenotype, organoids isolated from Maf knockout mice and treated with RANKL exhibited impaired M cell development and a significant decrease in Gp2 expression. These findings reveal that Maf is an important regulator for M cell development and differentiation.
Turning high-throughput structural biology into predictive inhibitor design
Authors: Saar Kadi L. , Fearon Daren ,   The COVID Moonshot Consortium, von Delft Frank , Chodera John D. , Lee Alpha A.
Published: October 2021

A common challenge in drug design pertains to finding chemical modifications to a ligand that increases its affinity to the target protein. An underutilised advance is the increase in structural biology throughput, which has progressed from an artisanal endeavour to a monthly throughput of up to 100 different ligands against a protein in modern synchrotrons. However, the missing piece is a framework that turns high throughput crystallography data into predictive models for ligand design. Here we designed a simple machine learning approach that predicts protein-ligand affinity from experimental structures of diverse ligands against a single protein paired with biochemical measurements. Our key insight is using physics-based energy descriptors to represent protein-ligand complexes, and a learning-to-rank approach that infers the relevant differences between binding modes. We ran a high throughput crystallography campaign against the SARS-CoV-2 Main Protease (MPro), obtaining parallel measurements of over 200 protein-ligand complexes and the binding activity. This allows us to design a one-step library syntheses which improved the potency of two distinct micromolar hits by over 10-fold, arriving at a non-covalent and non-peptidomimetic inhibitor with 120 nM antiviral efficacy. Crucially, our approach successfully extends ligands to unexplored regions of the binding pocket, executing large and fruitful moves in chemical space with simple chemistry.
Diet-induced obese mice are resistant to improvements in cardiac function resulting from short-term adropin treatment
Authors: Thapa Dharendra , Xie Bingxian , Mushala Bellina A.S. , Zhang Manling , Manning Janet R. , Bugga Paramesha , Stoner Michael W. , Jurczak Michael J. , Scott Iain
Published: October 2021

Previous studies have shown that treatment with recombinant adropin, a circulating peptide secreted by the liver and brain, restores glucose utilization in the hearts of diet-induced obese mice. This restoration of fuel substrate flexibility, which is lost in obese and diabetic animals, has the potential to improve contractile function in the diabetic heart. Using an ex vivo approach, we examined whether short-term adropin treatment could enhance cardiac function in a mouse model of diet-induced obesity. Our study showed that acute adropin treatment reduces inhibitory phosphorylation of pyruvate dehydrogenase in primary neonatal cardiomyocytes, and leads to moderate improvements in ex vivo cardiac function in mice fed a low fat diet. Conversely, short-term exposure to adropin led to a small decrease in cardiac function in mice fed a long-term high fat diet. Insulin treatment did not significantly alter cardiac function in adropin treated hearts from either low or high fat diet mice, however acute adropin treatment did moderately restore downstream insulin signaling in high fat diet fed mice. Overall, these data suggest that in an ex vivo setting, acute adropin treatment alone is not sufficient to promote improved cardiac function in obese animals.
Characterization of Cell-cell Communication in Autistic Brains with Single Cell Transcriptomes
Authors: Astorkia Maider , Lachman Herbert M. , Zheng Deyou
Published: October 2021

Autism spectrum disorder is a neurodevelopmental disorder, affecting 1-2% of children. Studies have revealed genetic and cellular abnormalities in the brains of affected individuals, leading to both regional and distal cell communication deficits. Recent application of single cell technologies, especially single cell transcriptomics, has significantly expanded our understanding of brain cell heterogeneity and further demonstrated that multiple cell types and brain layers or regions are perturbed in autism. The underlying high-dimensional single cell data provides opportunities for multi-level computational analysis that collectively can better deconvolute the molecular and cellular events altered in autism. Here, we apply advanced computation and pattern recognition approaches on single cell RNA-seq data to infer and compare inter-cell-type signaling communications in autism brains and controls. Our results indicate that at a global level there are cell-cell communication differences in autism in comparison to controls, largely involving neurons as both signaling senders and receivers, but glia also contribute to the communication disruption. Although the magnitude of change is moderate, we find that excitatory and inhibitor neurons are involved in multiple intercellular signaling that exhibit increased strengths in autism, such as NRXN and CNTN signaling. Not all genes in the intercellular signaling pathways are differentially expressed, but genes in the pathways are enriched for axon guidance, synapse organization, neuron migration, and other critical cellular functions. Furthermore, those genes are highly connected to and enriched for genes previously associated with autism risks. Overall, our proof-of-principle computational study using single cell data uncovers key intercellular signaling pathways that are potentially disrupted in the autism brains, suggesting that more studies examining cross-cell type affects can be valuable for understanding autism pathogenesis.
Transcriptomic responses of Galápagos finches to avian pox virus infection
Authors: McNew Sabrina M. , Yepez Janaí , Loyola C. Diana , Andreadis Catherine , Fessl Birgit
Published: October 2021

Emerging pathogens can have devastating effects on naïve hosts, but disease outcomes often vary among hosts. Comparing the cellular response of different host species to infection can provide insight into mechanisms of host defense and the basis of host susceptibility to disease. Here, we used RNA-seq to characterize the transcriptomic response of Darwin’s finches to avian poxvirus, which is introduced to the Galápagos Islands. We tested whether gene expression differs between infected and uninfected birds, and whether transcriptomic differences were related either to known antiviral mechanisms and/or the co-option of the host cellular environment by the virus. We compared two species, the medium ground finch (Geospiza fortis) and the vegetarian finch (Platyspiza crassirostris), to determine whether related species have similar responses to the same novel pathogen. We found that medium ground finches had a strong transcriptomic response to infection, upregulating genes involved in the innate immune response including interferon production, inflammation, and other immune signaling pathways. In contrast, vegetarian finches had a more limited response to infection. Our results also revealed evidence of viral manipulation of the host’s cellular function and metabolism, providing insight into the ways in which poxviruses affect their hosts. Many of the transcriptomic responses to infection mirrored known processes seen in model and in-vitro studies of poxviruses indicating that many pathways of host defense against poxviruses are conserved among vertebrates and present even in hosts without a long evolutionary history with the virus. At the same time, the variation we observed between closely related species indicates that some endemic species of Galápagos finch may be more susceptible to avian pox than others.
Tiam1-mediated synaptic plasticity drives comorbid depressive symptoms in chronic pain
Authors: Ru Qin , Lu Yungang , Saifullah Ali Bin , Blanco Francisco A , Yao Changqun , Cata Juan P , Li De-Pei , Tolias Kimberley F , Li Lingyong
Published: October 2021

Hyperactivity in the anterior cingulate cortex (ACC) drives comorbid depressive symptoms in chronic pain, but the cause of ACC hyperactivity is currently unclear. Ketamine, an N-methyl-D-aspartate receptor (NMDAR) antagonist, induces rapid and sustained antidepressant-like effects in chronic pain-induced depression in both patients and animal models. However, the mechanisms underlying ketamine’s sustained antidepressant effects remain elusive. Here, we show that Tiam1, a Rac1-specific guanine nucleotide exchange factor (GEF) that was previously identified as a critical mediator of NMDAR-dependent dendritic spine development, is activated in the ACC in chronic pain mice displaying depressive-like behaviors. Conditional deletion of Tiam1 from postnatal forebrain excitatory neurons, specific deletion of Tiam1 from ACC neurons, or pharmacological inhibition of the Tiam1-Rac1 signaling pathway prevents chronic pain-induced depressive-like behaviors in mice. Biochemical, morphological, and electrophysiological assays reveal that Tiam1 orchestrates synaptic structural and functional remodeling in ACC neurons via actin cytoskeleton reorganization and synaptic NMDAR stabilization. This Tiam1-coordinated synaptic plasticity underpins ACC hyperactivity and drives chronic pain-induced depressive-like behaviors. Ketamine induces sustained antidepressant effects in chronic pain by blocking Tiam1-mediated synaptic structural and functional plasticity in ACC neurons. Our results reveal Tiam1 as a key factor in the pathophysiology of chronic pain-induced depression and in the sustained antidepressant effects of ketamine in ACC neurons. These findings highlight Tiam1 as a potential therapeutic target for the treatment of comorbid depressive symptoms in chronic pain.
CFD analysis of variable geometric angle winglets
Authors: Kazim Ali Hussain , Malik Abdullah Hamid , Ali Hammad , Raza Muhammad Usman , Khan Awais Ahmad , Aized Tauseef , Shabbir Aqsa
Published: October 2021

Purpose

Winglets play a major role in saving fuel costs because they reduce the lift-induced drag formed at the wingtips. The purpose of this paper is to obtain the best orientation of the winglet for the Office National d’Etudes et de Recherches Aérospatiales (ONERA) M6 wing at Mach number 0.84 in terms of lift to drag ratio.

Design/methodology/approach

A computational fluid dynamics analysis of the wing-winglet configuration based on the ONERA M6 airfoil on drag reduction for different attack angles at Mach 0.84 was performed using analysis of systems Fluent. First, the best values of cant and sweep angles in terms of aerodynamic performance were selected by performing simulations. The analysis included cant angle values of 30°, 40°, 45°, 55°, 60°, 70° and 75°, while for the sweep angles 35°, 45°, 55°, 65° and 75° angles were used. The aerodynamic performance was measured in terms of the obtained lift to drag ratios.

Findings

The results showed that slight alternations in the winglet configuration can improve aerodynamic performance for various attack angles. The best lift to drag ratio for the winglet was achieved at a cant angle of 30° and a sweep angle of 65°, which caused a 5.33% increase in the lift to drag ratio. The toe-out angle winglets as compared to the toe-in angles caused the lift to drag ratio to increase because of more attached flow at its surface. The maximum value of the lift to drag ratio was obtained with a toe-out angle (−5°) at an angle of attack 3° which was 2.53% greater than the zero-toed angle winglet.

Originality/value

This work is relatively unique because the cant, sweep and toe angles were analyzed altogether and led to a significant reduction in drag as compared to wing without winglet. The wing model was compared with the results provided by National Aeronautics and Space Administration so this validated the simulation for different wing-winglet configurations.
Assessing the financial condition and accounts receivable risk among US farmer cooperatives
Authors: Mashange Gerald , Briggeman Brian C.
Published: October 2021

Purpose

The purpose of this paper is to examine the financial condition and ability of farmer cooperatives to withstand significant increases in bad debt expense.

Design/methodology/approach

A unique data set of farmer cooperative financial statements that spans from 1996 to 2019 is used to examine the changes in profitability, solvency, liquidity and accounts receivable risk. Also, a deterministic stress test model is designed to shock bad debt expense and the resulting write-off of accounts receivable for farmer cooperatives. The stress test provides insights to the resiliency of farmer cooperatives.

Findings

Results find that farmer cooperatives are in a strong financial position, which has improved over time. The majority of farmer cooperatives are able to absorb a substantial increase in bad debt expense because of their sizable, retained earnings position. However, cooperatives that have significant profitability challenges do experience much larger losses, especially mixed farmer cooperatives (roughly equally amounts of grain and farm supply sales) and large cooperatives with more than $500 million in sales.

Practical implications

The stress test results suggest farmer cooperative managers and boards of directors could re-examine their credit policies and consider extending additional credit. Also, cooperatives should consider monitoring and identifying an optimal accounts receivable to retained earnings ratio, which is similar to how banks examine their tier 1 capital ratios.

Originality/value

The value of this study is having data that allows for the examination of the financial condition of farmer cooperatives over time. Also, having current data means the accounts receivable stress test results are more relevant and timelier. This is important because these accounts receivable are primarily tied to crop input supplies, and farmer cooperatives are a significant market participant in the crop input supply market.
Predicting hybrid entrepreneurship among secondary school teachers in Nigeria
Authors: Adebusuyi Adeola Samuel , Adebusuyi Olubusayo Foluso
Published: October 2021

Purpose

The purpose of this study is to investigate how degree-holding secondary school teachers cope in a recessive economy by embracing hybrid entrepreneurship (HE). Specifically, we investigated how comparison with referent others, underemployment and relative deprivation led to HE.

Design/methodology/approach

The study adopted a cross-sectional research design. We used snowball and purposive sampling techniques to recruit 303 bachelor’s degree holders teaching in Nigerian public secondary schools in two states of the federation (Ondo and Ekiti states). We analyzed the data with regression path analysis and controlled for age and gender.

Findings

The results of this study showed the following. First, teachers were high in the feeling of pay underemployment and relative deprivation. Second, pay underemployment and relative deprivation directly led to HE. Third, teachers were indirectly high in HE through either pay underemployment or relative deprivation. Finally, underemployment and relative deprivation serially mediate the relationship between referent others and HE.

Research limitations/implications

Overall, the results suggest that teachers’ involvement in HE is necessity-driven to cope with the recessive Nigerian economy. However, future research should focus on a more experimental approach to determine the cause-effect relationship.

Originality/value

This is the first study to investigate how workers embrace HE to cope with the consequences of a recessive economy.
The rough road towards accounting harmonization of a developing country with a French accounting culture
Authors: Ben Slama Fatma , Oussii Ahmed Atef , Klibi Mohamed Faker
Published: October 2021

Purpose

The purpose of this paper is to investigate in-depth and explain the issues related to the experience of Tunisia, a developing country, in its attempt to move from Euro-Continental rule-based generally accepted accounting principles (GAAPs) to an accounting system adapted to international financial reporting standards (IFRS).

Design/methodology/approach

The study is conducted via a qualitative methodology based on a content analysis of primary data from interviews with key actors involved in financial reporting in Tunisia.

Findings

Findings reveal that local Tunisian GAAPs, adapted to IFRS in their 1996 version, failed to establish a financial reporting accounting culture and meet public-interest firms’ informational needs. This is mainly related to factors, such as the simplified methods adopted (generally adequate to the identified needs of users of small and medium-sized entity financial statements) and the hybrid aspect of the Tunisian accounting standards due to the co-existence of Euro-Continental and Anglo-Saxon parties. Moreover, the findings show that the lack of political willpower and the absence of updates to changes in IFRS have compromised the proper functioning of standardization and control structures.

Practical implications

The study’s results may interest regulators and policymakers of many developing countries that have not pursued the harmonization of their local GAAPs with IFRS. In addition, findings from the research provide insights into the rough road towards harmonization, the dysfunctions of the latter and delays in developing countries.

Originality/value

The research highlights the complexity for an emerging country with Euro-Continental accounting traditions to move to IFRS.
Do young consumers care about ethical consumption? Modelling Gen Z's purchase intention towards fair trade coffee
Authors: Robichaud Zachary , Yu Hong
Published: October 2021

Purpose

A global shift in ethical/sustainable purchase drivers highlights Generation Z (persons aged 15–24) as an important market for producers and marketers. Although much research has touched on fair trade consumption, very little has focused on Gen Z's consumption patterns. This study provides insights into and implications of younger consumers' motivations in ethical/sustainable consumption.

Design/methodology/approach

This research examines Gen Z's purchase intention towards fair trade coffee with the theory of reasoned action framework. Data were collected with a convenience sample, and analyses were conducted using structural equation modelling.

Findings

The research found a significant influence of knowledge of fair trade towards product interest. Furthermore, general attitudes towards fair trade had a significant influence on product interest, product likeability and convenience. Lastly, product interest and subjective norms significantly influenced Gen Z's purchase intentions towards fair trade coffee.

Originality/value

Findings suggest that Gen Z's shift in ethical/sustainable consumption revolves around their subjective norms or peer influence circles and contributes to the notions of self-branding, identify claims and social currency. Younger generations are digital natives, and social media has created a looking glass into their actions. This digital expansion has created more opportunities for individuals to monitor the actions of others and release information in real-time. Therefore, ethical/sustainable consumption by Gen Z can be used as a communication tool among their peers to project personal values and ideological shifts and to influence others close to them.
Assessment of systemic and carcinogenic health risks of persistent organochlorine pesticide residues in four fruit vegetables in south-western Nigeria
Authors: Odewale Gideon Oluwasogo , Sosan Mosudi Babatunde , Oyekunle John Adekunle Oyedele , Adeleye Adeoluwa Oluwaseyi
Published: October 2021

Purpose

The study assessed the levels of organochlorine pesticides (OCPs) and their potential non-carcinogenic and carcinogenic health risks in four regularly consumed fruit vegetables.

Design/methodology/approach

The OCPs’ residues were quantified using a gas chromatograph coupled with an electron capture detector (GC-ECD) and the dietary exposure of children, and adult consumers to the detected OCPs was evaluated using carcinogenic and systemic health risk estimations.

Findings

Aldrin, endrin, endrin aldehyde, a-endosulfan, β-endosulfan, endosulfan sulphate, heptachlor, heptachlor epoxide and dieldrin were detected in the four fruit vegetables. The predominant OCP residue in carrot, cucumber, tomatoes and watermelon was endosulfan sulphate with mean concentrations of 2.532 mg kg−1, 1.729 mg kg−1, 2.363 mg kg−1 and 1.154 mg kg−1, respectively. The residues levels in some of the fruit vegetables were higher than their respective maximum residue levels (MRLs) of 0.01–0.05 mg kg−1 set by the European Commission with concentrations above MRLs ranging between 25.5% and 100%. The systemic health risk estimations showed that the hazard index (HI) values for carrot (3.20), cucumber (9.25), tomatoes (50.21) and watermelon (16.76) were >1 for children consumers and the respective HI values of 2.87, 15.57 and 5.20 for adult consumers of cucumber, tomatoes and watermelon were >1 which implies potential systemic health risks. Four carcinogens (aldrin, dieldrin, heptachlor and heptachlor epoxide) had cancer risk index values greater than the acceptable risk of 1 in 1 million for both adult and children consumers.

Originality/value

The paper shows that despite the ban on the use of OCPs in Nigeria, they are still being used for agricultural production especially on some locally produced and regularly consumed fruit vegetables as reported in the present study. The non-existence of surveillance programmes on pesticide usage and the lack of proper monitoring of pesticide residues in food products including fruit and vegetables must have contributed to the levels of the detected OCP in the samples analysed. The current daily intake of OCP via contaminated vegetables may pose potential health risks to both the children and adult consumers of the fruit vegetables.
Exploring the benefits of service productisation: support for business processes
Authors: Harkonen Janne
Published: October 2021

Purpose

The study aims to explore the benefits of service productisation to provide further understanding on the productisation concept as support for business processes and service management. The concept has been deficiently discussed regardless of the potential significance to the whole formed by service products, business processes, information technology (IT), people and data.

Design/methodology/approach

In the study, the exploratory empirical evidence is presented from 16 cases, 4 of which are from companies that are globally well-known.

Findings

The key findings of the paper include an overview of the benefits of service productisation and the relation to service offering, service processes and related resources. The concept links to the management of the whole formed by service products, business processes, IT, people and data. The noted benefits seem to be applicable to productisation of different service types, whilst some service characteristics may affect the specific emphasis.

Research limitations/implications

The limitations involve using secondary data, which, however, makes the cases less biased regarding the aims. Primary data are required to gain further insights into the phenomena and the identified benefits.

Practical implications

The findings provide support for issues that are commonly discussed by practitioners on a concept that is less studied by the scientific literature. Practitioners can work towards organisational efficiency and effectiveness by understanding the benefits of productisation. Understanding service productisation can support the effective management of business processes and work towards prosperity in the service business.

Originality/value

The study is the first one to analyse the benefits of service productisation by exploring the issue through multiple cases and attempting to identify aspects for further attention by the academic community.
Research productivity of International Financial Reporting Standards (IFRS) from 2003 to 2020
Authors: Tijjani Bashir , Ur Rehman Shafiq , Peter Zachariah , Bajwa Ishtiaq Ahmad , Khan Muhammad Ajmal
Published: October 2021

Purpose

This study aims to examine the quantitative research productivity of International Financial Reporting Standards (IFRS) globally by using the bibliometric approach. The method was applied to articles indexed in the Scopus database to analyze the publication patterns, trends and research productivity of the selected papers.

Design/methodology/approach

Bibliometric analysis is applied to analyze research productivity of IFRS from 2003 to 2020. The method was applied to articles indexed in the Scopus database to analyze the publication patterns and research productivity of the selected papers.

Findings

This study finds that a good number of articles have been published on IFRS, the top five countries are the USA, UK, Australia, Germany and Canada. This clearly shows that developed markets have the highest number of publications on IFRS. This could be as a result of the early adoption of IFRS by those economies and owing to the interest of researchers in those markets. Most of the studies are quantitative in nature; this study indicates that publication on accounting standards is popular as the number of citations is significant; most of the articles have two or more authors and were published in top-ranking journals.

Practical implications

This study provides up-to-date literature on the global research productivity of IFRS; as a result, it supports the development of policies by the users of this accounting standards. The findings of this study also serve as a reference point for firms and regulators around the world. Given the thoroughness of the methodology of this study, the results make it easier to effectively identify the direction of research on the implementation of IFRS in organizations.

Originality/value

This study provides a more comprehensive bibliometric analysis on the growth of IFRS literature (2003–2020) in the Scopus database; most of the prior studies have covered relatively few areas of focus as well as a fewer number of high impact factor journals. The relevance of this finding is in uncovering different areas of IFRS research productivity globally.
Interaction between wealth management products and bank deposits: evidence from China's shadow banking
Authors: Shah Syed Mehmood Raza , Lu Yan , Fu Qiang , Ishfaq Muhammad , Abbas Ghulam
Published: October 2021

Purpose

Shadow banking has been evolving rapidly in China, with banks actively using wealth management products (WMPs) to evade regulatory restrictions. These products are the largest constituent of China's shadow banking sector. A large number of these products are off-balance-sheet and considered a substitute for bank deposits. China's banking sector, especially the small and medium-sized banks (SMBs), uses these products to avoid regulatory restrictions and sustainability risk in the deposit market.

Design/methodology/approach

This study empirically examined how banks in China, specifically SMBs, utilize these products on a short and long-run basis to manage and control their deposit levels. This study utilized a quarterly panel dataset from 2010 to 2019 for the top 30 Chinese banks, by first implementing a Panel ARDL-PMG model. For cross-sectional dependence, this study further executed a cross-sectional augmented autoregressive distributive lag model (CS-ARDL).

Findings

Under regulations avoidance theory, the findings revealed that WMPs and deposits have a stable long-run substitute relationship. Furthermore, the WMP–Deposit substitute relationship was only significant and consistent for SMBs, but not for large four banks. The findings further revealed that the WMP–Deposit substitute relationship existed, even after the removal of the deposit rate limit imposed by the People's Bank of China (PBOC) to control the deposit rates.

Research limitations/implications

The individual bank-issued WMPs' amount data is not available in any database. Therefore, this study utilized the number of WMPs as a proxy for China's banking sector's exposure to the wealth management business.

Practical implications

This research helps policymakers to understand the Deposit–WMP relationship from the off-balance-sheet perspective. During the various stages of interest rate liberalization, banks were given more control to establish their deposit and loan interest rates. However, the deposit rates are still way below the WMP returns, making WMPs more competitive. This research suggests that policymakers should formulate a more balanced strategy regarding deposit rates and WMPs returns.

Originality/value

This study contributes to the existing literature on China's shadow banking by concentrating on the WMPs. This research represents one of the few studies that analyze regulatory arbitrage in terms of the WMP–Deposit relationship. Moreover, the implementation of CS-ARDL panel data models and multiple data sources makes this study's findings more reliable and significant.
Addressing endogeneity: some (mostly) harmless recommendations
Authors: Assaf A. George , Tsionas Mike
Published: October 2021

Purpose

This paper aims to focus on addressing endogeneity using instrument-free methods. The authors discuss some extensions to well-known techniques.

Design/methodology/approach

This paper discusses some attractive methods to address endogeneity without the need for instruments. The methods are labeled are “harmless” in the sense that instruments are not needed and the distributional assumptions are kept to a minimum or they are replaced by more flexible semi-parametric assumptions.

Findings

Using a hospitality application, the authors provide evidence about the effectiveness of these techniques and provide directions for their implementation.

Research limitations/implications

Finding valid instruments has always been a key challenge for researchers in the field. This paper discusses and introduces methods that free researchers from the need to find instruments.

Originality/value

The paper discusses techniques that are introduced from the first time in the tourism literature.
Next-generation business models for artificial intelligence start-ups in the healthcare industry
Authors: Kulkov Ignat
Published: October 2021

Purpose

Value creation based on artificial intelligence (AI) can significantly change global healthcare. Diagnostics, therapy and drug discovery start-ups are some key forces behind this change. This article aims to study the process of start-ups' value creation within healthcare.

Design/methodology/approach

A multiple case study method and a business model design approach were used to study nine European start-ups developing AI healthcare solutions. Obtained information was performed using within and cross-case analysis.

Findings

Three unique design elements were established, with 16 unique frames and three unifying design themes based on business models for AI healthcare start-ups.

Originality/value

Our in-depth framework focuses on the features of AI start-up business models in the healthcare industry. We contribute to the business model and business model innovation by systematically analyzing value creation, how it is delivered to customers, and communication with market participants, as well as design themes that combine start-ups and categorize them by specialization.
An evaluation of Lean and Six Sigma methodologies in the national health service
Authors: Antony Jiju , Lancastle James , McDermott Olivia , Bhat Shreeranga , Parida Ratri , Cudney Elizabeth A.
Published: October 2021

Purpose

The purpose of this paper is to conduct an empirical study derived from the previous literature from the perspective of benefits, tools and techniques, continuous improvement (CI) and quality improvement (QI) methodologies and critical failure factors (CFFs) of Lean and Six Sigma (SS) in the national health service (NHS).

Design/methodology/approach

A literature review was carried out to identify previous findings, empirical data and critical variables concerning Lean and SS in healthcare for over ten years. Second, primary research in quantitative surveys and qualitative interviews was carried out with 110 participants who have experience using Lean and SS in the NHS.

Findings

Lean and SS have evolved into common practices within the NHS and now have an established list of tools and techniques frequently employed by staff. Lean and SS are considered robust CI methodologies capable of effectively delivering extensive benefits across many different categories. The NHS must overcome a sizable amount of highly important CFFs and divided organizational culture.

Originality/value

This paper has developed the most extensive empirical study ever produced on Lean and SS in the NHS and has expanded on previous works to create new and updated research. The findings produced in this paper will assist NHS medical directors and practitioners in obtaining up-to-date insight into Lean and SS status in the NHS. The paper will also guide the NHS to critically evaluate their current CI strategy to ensure long-term sustainability and deliver improved levels of service to patients.
Porosity formation studies in high pressure die castings of Al-9Si-3Cu alloy based on Taguchi method
Authors: Appa Rao K. Ch , Birru Anil Kumar , Bannaravuri Praveen Kumar , Francis E. Daniel
Published: October 2021

Purpose

Nowadays, ample industries are fascinated to look for high strength and light weight materials for the development of robust parts. Because of light weight and high stiffness to weight ratio; usage of aluminum parts is growing rapidly, especially in automotive engineering. Process improvement of Al alloys and their grain structure refinement is the current area of interest in casting companies. In this research work, an investigation has been carried out to enhance the process improvement of die casting by optimization of various significant parameters and their refinement of grains by the effect of Nb-C novel grain refiner.

Design/methodology/approach

L27 orthogonal array (OA) has been considered to optimize the preferred casting input parameters such as molten metal temperature (°C), die temperature (°C), injection pressure (bar), Al-3.5Nb-1.5 C novel grain refiner and Ni alloying additions as key process parameters in order to increase the quality and efficiency of Al-9Si-3Cu aluminum alloy die casting by reducing the porosity formation.

Findings

It was observed that the porosity values have significantly decreased from 0.88% to 0.25% particularly at 0.1 wt.% of new grain refiner and 0.5 wt. % of Al-6Ni master alloy. As per the ANOVA results, it was observed that Al-3.5FeNb-1.5 C grain refiner (F value 2609.22), Al-6Ni alloying addition (F value 1329.13), molten metal temperature (F value 1002.43) and, injection pressure (F value 448.06) are the factors that significantly affects the porosity, whereas die temperature was found to be insignificant. The results show that new grain refiner is one the most significant factor among the other selected parameters. The contribution of the new grain refiner to the variation of mean casting porosity is around 57.74%. confidence interval (CI) has also been estimated as 0.013 for 95% consistency level to validate the predicted range of optimum casting porosity of aforesaid alloy.

Originality/value

To the best of the authors' knowledge, no study has been conducted in the past to investigate the combined effect of these die casting parameters and composition factors for the development of Al-Si robust cast parts. The paper represents original research and provides new information for the fabrication of die casting parts.
Negative emotional consequences of labour market activation policies for long-term unemployed young adults in Finland
Authors: Ylistö Sami , Husu Hanna-Mari
Published: October 2021

Purpose

This article deals with the negative emotional consequences of active labour market policies (ALMPs) for long-term unemployed young adults in Finland. Although such policies may have positive effects, an exploration of their negative impacts reveals their problematic side effects. We explore various aspects of ALMP interventions that prevent individuals from gaining such positive outcomes and thus reduce their motivation to invest in the policies.

Design/methodology/approach

Drawing on the affect theory of social exchange, we understand that individuals seek positive rewards from social interactions. Our data is taken from life course interviews with unemployed people aged 20–31 in central Finland in 2012–2013.

Findings

We find three factors linked to ALMPs that diminish participants' emotional well-being: experiences of unfairness, lack of control and a mismatch between ALMPs and clients' needs. By paying attention to aspects of labour market policy that diminish emotional well-being, it is possible to build more functional policies that better meet the needs of long-term unemployed individuals.

Originality/value

This study fills a significant gap in the literature, because there is limited research on unintended negative outcomes of ALMP activation.
A descriptive analysis of three-generation households and mothers' employment in Japan, 2002–2019
Authors: Miyazaki Rie
Published: October 2021

Purpose

This article aims to explore how Japanese women with younger children changed their commitment to the labour market between 2000 and 2019 by comparing mothers in three-generation and nuclear family households.

Design/methodology/approach

Japan currently has the highest ageing rate in the world at nearly 30%. Since the 1990s, employment flexibilization and women's labour market participation have proceeded in parallel, and the conservative family values of the patriarchy and gender division of labour that have provided intergenerational aid for care within households have been shrinking, by conducting a descriptive analysis of the Labour Force Survey (LFS).

Findings

This study identified that a conspicuous increase in part-time employment among mothers in both household types and a decrease by half in the working mother's population in three-generation households. These results suggest that the function of inter-generational assistance by multi-generation cohabitation, which was once thought to be effective in helping working mothers with younger children, is declining.

Originality/value

A study examining the transformation of mothers' employment behaviour differences between three-generation households and nuclear family households is rare. This paper makes a new contribution to the research regarding the grandparents' caregiving, household types and mothers' employment.
The effect of parental roles on mental health outcomes of unemployment: a gender perspective
Authors: Russo Concetta , Decataldo Alessandra , Terraneo Marco
Published: October 2021

Purpose

This paper aims to investigate the extent to which family roles and settings can mediate the impact of unemployment on psychological well-being among Italian households.

Design/methodology/approach

Using the European Health Interview Survey data for 2015, the authors adopt linear regression models to evaluate the effect of family settings on the mental health outcomes of unemployment, in particular on the likelihood of developing depression. The latter is measured using the internationally validated Patient Health Questionnaire (PHQ-8). Since the gender gap in occupation has not ceased to exist in Italy, special attention is paid to the differences between males and females in the workforce.

Findings

The results suggest that involvement in parental roles has a moderating influence on unemployment mental health outcomes among both men and women, although it has a higher effect on the female workforce. Moreover, the study shows that “not living far from the family of origin” could be considered a crucial moderating factor for both gender categories.

Originality/value

The novelty of this paper lies in its consideration of the implications the social definition of gender roles may have on gender-related expectancies and attributions in life domains, such as work and family.