bluuebunny/crossref_metadata_2025 huggingface dataset:
Vagus Nerve Stimulation with Mild Stimulation Intensity Exerts Anti-Inflammatory and Neuroprotective Effects in Parkinson’s Disease Model Rats
Authors: Kin Ittetsu , Sasaki Tatsuya , Yasuhara Takao , Kameda Masahiro , Agari Takashi , Okazaki Mihoko , Hosomoto Kakeru , Okazaki Yosuke , Yabuno Satoru , Kawauchi Satoshi , Kuwahara Ken , Morimoto Jun , Kin Kyohei , Umakoshi Michiari , Tomita Yousuke , Tajiri Naoki , Borlongan Cesario , Date Isao
Published: July 2021

Background: The major surgical treatment for Parkinson’s disease (PD) is deep brain stimulation (DBS), but a less invasive treatment is desired. Vagus nerve stimulation (VNS) is a relatively safe treatment without cerebral invasiveness. In this study, we developed a wireless controllable electrical stimulator to examine the efficacy of VNS on PD model rats. Methods: Adult female Sprague-Dawley rats underwent placement of a cuff-type electrode and stimulator on the vagus nerve. Following which, 6-hydroxydopamine (6-OHDA) was administered into the left striatum to prepare a PD model. VNS was started immediately after 6-OHDA administration and continued for 14 days. We evaluated the therapeutic effects of VNS with behavioral and immunohistochemical outcome assays under different stimulation intensity (0.1, 0.25, 0.5 and 1 mA). Results: VNS with 0.25–0.5 mA intensity remarkably improved behavioral impairment, preserved dopamine neurons, reduced inflammatory glial cells, and increased noradrenergic neurons. On the other hand, VNS with 0.1 mA and 1 mA intensity did not display significant therapeutic efficacy. Conclusions: VNS with 0.25–0.5 mA intensity has anti-inflammatory and neuroprotective effects on PD model rats induced by 6-OHDA administration. In addition, we were able to confirm the practicality and effectiveness of the new experimental device.
Occluded Superior Mesenteric Artery and Vein. Therapy with the Stable Gastric Pentadecapeptide BPC 157
Authors: Knezevic Mario , Gojkovic Slaven , Krezic Ivan , Zizek Helena , Malekinusic Dominik , Vrdoljak Borna , Knezevic Tamara , Vranes Hrvoje , Drmic Domagoj , Staroveski Miro , Djuzel Antonija , Rajkovic Zoran , Kolak Toni , Lovric Eva , Milavic Marija , Sikiric Suncana , Tvrdeic Ante , Patrlj Leonardo , Strbe Sanja , Sola Marija , Situm Andrej , Kokot Antonio , Boban Blagaic Alenka , Skrtic Anita , Seiwerth Sven , Sikiric Predrag
Published: July 2021

Background. We investigated the occluded essential vessel tributaries, both arterial and venous, occluded superior mesenteric vein and artery in rats, consequent noxious syndrome, peripherally and centrally. As therapy, we hypothesized the rapidly activated alternative bypassing pathways, arterial and venous, and the stable gastric pentadecapeptide BPC 157 since it rapidly alleviated venous occlusion syndromes. Methods. Assessments were performed for 30 min (gross recording, venography, ECG, pressure, microscopy, biochemistry, and oxidative stress), including portal hypertension, caval hypertension, aortal hypotension, and centrally, the superior sagittal sinus hypertension; systemic arterial and venous thrombosis, ECG disturbances, MDA-tissue increase, the multiple organs lesions, heart, lung, liver, kidney and gastrointestinal tract, including brain (swelling, and cortex (cerebral, cerebellar), hypothalamus/thalamus, hippocampus lesions). Rats received BPC 157 medication (10 µg/kg, 10 ng/kg) intraperitoneally at 1 min ligation-time. Results. BPC 157 rapidly activated collateral pathways. These collateral loops were the superior mesenteric vein-inferior anterior pancreaticoduodenal vein-superior anterior pancreaticoduodenal vein-pyloric vein-portal vein pathway, an alternative pathway toward inferior caval vein via the united middle colic vein and inferior mesenteric vein through the left colic vein, and the inferior anterior pancreaticoduodenal artery and inferior mesenteric artery. Consequently, BPC 157 counteracted the superior sagittal sinus, portal and caval hypertension, aortal hypotension, progressing venous and arterial thrombosis peripherally and centrally, ECG disturbances attenuated. Markedly, the multiple organs lesions, heart, lung, liver, kidney, and gastrointestinal tract, in particular, as well as brain lesions, and oxidative stress in tissues were attenuated. Conclusions. BPC 157 therapy rapidly recovered rats, which have complete occlusion of the superior mesenteric vein and artery.
In Vitro Evaluation of a Nanoparticle-Based mRNA Delivery System for Cells in the Joint
Authors: Sturm Lisa , Schwemberger Bettina , Menzel Ursula , Häckel Sonja , Albers Christoph E. , Plank Christian , Rip Jaap , Alini Mauro , Traweger Andreas , Grad Sibylle , Basoli Valentina
Published: July 2021

Biodegradable and bioresponsive polymer-based nanoparticles (NPs) can be used for oligonucleotide delivery, making them a promising candidate for mRNA-based therapeutics. In this study, we evaluated and optimized the efficiency of a cationic, hyperbranched poly(amidoamine)s-based nanoparticle system to deliver tdTomato mRNA to primary human bone marrow stromal cells (hBMSC), human synovial derived stem cells (hSDSC), bovine chondrocytes (bCH), and rat tendon derived stem/progenitor cells (rTDSPC). Transfection efficiencies varied among the cell types tested (bCH 28.4% ± 22.87, rTDSPC 18.13% ± 12.07, hBMSC 18.23% ± 14.80, hSDSC 26.63% ± 8.81) and while an increase of NPs with a constant amount of mRNA generally improved the transfection efficiency, an increase of the mRNA loading ratio (2:50, 4:50, or 6:50 w/w mRNA:NPs) had no impact. However, metabolic activity of bCHs and rTDSPCs was significantly reduced when using higher amounts of NPs, indicating a dose-dependent cytotoxic response. Finally, we demonstrate the feasibility of transfecting extracellular matrix-rich 3D cell culture constructs using the nanoparticle system, making it a promising transfection strategy for musculoskeletal tissues that exhibit a complex, dense extracellular matrix.
Antisense Oligonucleotide-Based Therapeutic against Menin for Triple-Negative Breast Cancer Treatment
Authors: Nguyen Dang Tan , Le Thi Khanh , Paris Clément , Cherif Chaïma , Audebert Stéphane , Udu-Ituma Sandra Oluchi , Benizri Sébastien , Barthélémy Philippe , Bertucci François , Taïeb David , Rocchi Palma
Published: July 2021

The tumor suppressor menin has dual functions, acting either as a tumor suppressor or as an oncogene/oncoprotein, depending on the oncological context. Triple-negative breast cancer (TNBC) is characterized by the lack of expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (ERBB2/HER2) and is often a basal-like breast cancer. TNBC is associated with a dismal prognosis and an insufficient response to chemotherapies. Previously, menin was shown to play a proliferative role in ER-positive breast cancer; however, the functions of menin in TNBC remain unknown. Here, we have demonstrated that menin is expressed in various TNBC subtypes with the strongest expression in the TNBC Hs 578T cells. The depletion of menin by an antisense oligonucleotide (ASO) inhibits cell proliferation, enhances apoptosis in Hs 578T cells, highlighting the oncogenic functions of menin in this TNBC model. ASO-based menin silencing also delays the tumor progression of TNBC xenografts. Analysis of the menin interactome suggests that menin could drive TNBC tumorigenesis through the regulation of MLL/KMT2A-driven transcriptional activity, mRNA 3′-end processing and apoptosis. The study provides a rationale behind the use of ASO-based therapy, targeting menin in monotherapy or in combination with chemo or PARP inhibitors for menin-positive TNBC treatments.
Myeloid HIF1α Is Involved in the Extent of Orthodontically Induced Tooth Movement
Authors: Kirschneck Christian , Straßmair Nadine , Cieplik Fabian , Paddenberg Eva , Jantsch Jonathan , Proff Peter , Schröder Agnes
Published: July 2021

During orthodontic tooth movement, transcription factor hypoxia-inducible factor 1α (HIF1α) is stabilised in the periodontal ligament. While HIF1α in periodontal ligament fibroblasts can be stabilised by mechanical compression, in macrophages pressure application alone is not sufficient to stabilise HIF1α. The present study was conducted to investigate the role of myeloid HIF1α during orthodontic tooth movement. Orthodontic tooth movement was performed in wildtype and Hif1αΔmyel mice lacking HIF1α expression in myeloid cells. Subsequently, µCT images were obtained to determine periodontal bone loss, extent of orthodontic tooth movement and bone density. RNA was isolated from the periodontal ligament of the control side and the orthodontically treated side, and the expression of genes involved in bone remodelling was investigated. The extent of tooth movement was increased in Hif1αΔmyel mice. This may be due to the lower bone density of the Hif1αΔmyel mice. Deletion of myeloid Hif1α was associated with increased expression of Ctsk and Acp5, while both Rankl and its decoy receptor Opg were increased. HIF1α from myeloid cells thus appears to play a regulatory role in orthodontic tooth movement.
Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System
Authors: Gwiazda Marcin , Bhardwaj Sheetal K. , Kijeńska-Gawrońska Ewa , Swieszkowski Wojciech , Sivasankaran Unni , Kaushik Ajeet
Published: July 2021

This research presents an electrochemical immunosensor for collagen I detection using a self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) and covalently immobilized half-reduced monoclonal antibody as a receptor; this allowed for the validation of the collagen I concentration through two different independent methods: electrochemically by Electrochemical Impedance Spectroscopy (EIS), and optically by Surface Plasmon Resonance (SPR). The high unique advantage of the proposed sensor is based on the performance of the stable covalent immobilization of the AuNPs and enzymatically reduced half-IgG collagen I antibodies, which ensured their appropriate orientation onto the sensor’s surface, good stability, and sensitivity properties. The detection of collagen type I was performed in a concentration range from 1 to 5 pg/mL. Moreover, SPR was utilized to confirm the immobilization of the monoclonal half-antibodies and sensing of collagen I versus time. Furthermore, EIS experiments revealed a limit of detection (LOD) of 0.38 pg/mL. The selectivity of the performed immunosensor was confirmed by negligible responses for BSA. The performed approach of the immunosensor is a novel, innovative attempt that enables the detection of collagen I with very high sensitivity in the range of pg/mL, which is significantly lower than the commonly used enzyme-linked immunosorbent assay (ELISA).
Addendum: Perri et al. Electrochemotherapy as a First Line Treatment in Recurrent Squamous Cell Carcinoma of the Oral Cavity and Oropharynx PDL-1 Negative and/or with Evident Contraindication to Immunotherapy: A Randomized Multicenter Controlled Trial. Cancers 2021, 13, 2210
Authors: Perri Francesco , Longo Francesco , Fusco Roberta , D’Alessio Valeria , Aversa Corrado , Pavone Ettore , Pontone Monica , Marciano Maria Luisa , Villano Salvatore , Franco Pierluigi , Togo Giulia , De Fazio Gianluca Renato , Ordano Daniele , Maglitto Fabio , Salzano Giovanni , Maglione Maria Grazia , Guida Agostino , Ionna Franco
Published: July 2021

Comments: We have some additional considerations about the recent published article by Perri et al [...]
Microwave Ablation of Very-Early- and Early-Stage HCC: Efficacy Evaluation by Correlation with Histology after Liver Transplantation
Authors: Crocetti Laura , Scalise Paola , Bozzi Elena , Campani Daniela , Rossi Piercarlo , Cervelli Rosa , Bargellini Irene , Ghinolfi Davide , De Simone Paolo , Cioni Roberto
Published: July 2021

Microwave (MW) ablation is a worldwide-diffused technique for the percutaneous ablation of hepatocellular carcinoma (HCC). Nevertheless, the efficacy of this technique still needs to be confirmed in pathological specimens. The purpose of this study was to evaluate the efficacy of MW ablation by correlation with histology in excised liver samples at the time of liver transplantation (LT). All patients with MW-ablated HCC who subsequently underwent LT between 2012 and 2020 were retrospectively evaluated. In the explanted livers, the treated lesions were evaluated at pathology, and the necrosis was classified as complete or partial. Thirty-six HCCs were ablated in 30 patients (20.9 ± 6.1 mm, a range of 10–30 mm). Ablations were performed with a single insertion of a MW antenna under ultrasound or CT guidance. A complete radiological response was demonstrated in 30/36 nodules (83.3%) in 24/30 patients (80%) at imaging performed one-month after MW ablation. At pathology, of the 36 treated nodules, 28 (77.8%) showed a complete necrosis, and 8 (22.2%) showed a pathological partial necrosis. Good agreement was found between the imaging performed one-month after treatment and the complete pathological response (Cohen’s k = 0.65). The imaging accuracy in detecting a complete response to treatment was 88.9%. All lesions with complete necrosis did not show recurrence at follow-up imaging until transplantation. The rad-path correlation in the explanted livers showed that MW ablation achieved a high rate of complete necrosis if a macroscopical complete ablation was obtained.
Curcumin: Modulator of Key Molecular Signaling Pathways in Hormone-Independent Breast Cancer
Authors: Farghadani Reyhaneh , Naidu Rakesh
Published: July 2021

Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer death among women worldwide. Despite the overall successes in breast cancer therapy, hormone-independent HER2 negative breast cancer, also known as triple negative breast cancer (TNBC), lacking estrogens and progesterone receptors and with an excessive expression of human epidermal growth factor receptor 2 (HER2), along with the hormone-independent HER2 positive subtype, still remain major challenges in breast cancer treatment. Due to their poor prognoses, aggressive phenotype, and highly metastasis features, new alternative therapies have become an urgent clinical need. One of the most noteworthy phytochemicals, curcumin, has attracted enormous attention as a promising drug candidate in breast cancer prevention and treatment due to its multi-targeting effect. Curcumin interrupts major stages of tumorigenesis including cell proliferation, survival, angiogenesis, and metastasis in hormone-independent breast cancer through the modulation of multiple signaling pathways. The current review has highlighted the anticancer activity of curcumin in hormone-independent breast cancer via focusing on its impact on key signaling pathways including the PI3K/Akt/mTOR pathway, JAK/STAT pathway, MAPK pathway, NF-ĸB pathway, p53 pathway, and Wnt/β-catenin, as well as apoptotic and cell cycle pathways. Besides, its therapeutic implications in clinical trials are here presented.
Preclinical In Vivo-Models to Investigate HIPEC; Current Methodologies and Challenges
Authors: Helderman Roxan F. C. P. A. , Löke Daan R. , Tanis Pieter J. , Tuynman Jurriaan B. , Ceelen Wim , de Hingh Ignace H. , van der Speeten Kurt , Franken Nicolaas A. P. , Oei Arlene L. , Kok H. Petra , Crezee Johannes
Published: July 2021

Hyperthermic intraperitoneal chemotherapy (HIPEC) is a treatment modality for patients with peritoneal metastasis (PM) of various origins which aims for cure in combination with cytoreductive surgery (CRS). Efficacy of CRS-HIPEC depends on patient selection, tumor type, delivery technique, and treatment parameters such as temperature, carrier solution, type of drug, dosage, volume, and treatment duration. Preclinical research offers a powerful tool to investigate the impact of these parameters and to assist in designing potentially more effective treatment protocols and clinical trials. The different methodologies for peritoneal disease and HIPEC are variable. This study aims to review the objectives, methods, and clinical relevance of in vivo preclinical HIPEC studies found in the literature. In this review, recommendations are provided and possible pitfalls are discussed on the choice of type of animal and tumor model per stratified parameters and study goal. The guidelines presented in this paper can improve the clinical relevance and impact of future in vivo HIPEC experiments.
The Anti-VEGF(R) Drug Discovery Legacy: Improving Attrition Rates by Breaking the Vicious Cycle of Angiogenesis in Cancer
Authors: Ribatti Domenico , Solimando Antonio Giovanni , Pezzella Francesco
Published: July 2021

Resistance to anti-vascular endothelial growth factor (VEGF) molecules causes lack of response and disease recurrence. Acquired resistance develops as a result of genetic/epigenetic changes conferring to the cancer cells a drug resistant phenotype. In addition to tumor cells, tumor endothelial cells also undergo epigenetic modifications involved in resistance to anti-angiogenic therapies. The association of multiple anti-angiogenic molecules or a combination of anti-angiogenic drugs with other treatment regimens have been indicated as alternative therapeutic strategies to overcome resistance to anti-angiogenic therapies. Alternative mechanisms of tumor vasculature, including intussusceptive microvascular growth (IMG), vasculogenic mimicry, and vascular co-option, are involved in resistance to anti-angiogenic therapies. The crosstalk between angiogenesis and immune cells explains the efficacy of combining anti-angiogenic drugs with immune check-point inhibitors. Collectively, in order to increase clinical benefits and overcome resistance to anti-angiogenesis therapies, pan-omics profiling is key.
Steam Reforming of Chloroform-Ethyl Acetate Mixture to Syngas over Ni-Cu Based Catalysts
Authors: Wu Qiong , Xu Chenghua , Zheng Yuhao , Liu Jie , Deng Zhiyong , Liu Jianying
Published: July 2021

NiCuMoLaAl mixed oxide catalysts are prepared and applied in the steam reforming of chloroform-ethyl acetate (CHCl3-EA) mixture to syngas in the present work. The pre-introduction of Cl- ions using chloride salts as modifiers aims to improve the chlorine poisoning resistance. Catalytic tests show that KCl modification is obviously advantageous to increase the catalytic life. The destruction of catalyst structure induced by in situ produced HCl and carbon deposits that occurred on acidic sites are two key points for deactivation of reforming catalysts. The presence of Cl− ions gives rise to the formation of an Ni-Cu alloy, which exhibits a synergetic effect on catalyzing reforming along with metallic Ni crystals formed from excess nickel species, and giving an excellent catalytic stability. Less CHCl3 and more steam can also increase the catalytic stable time of KCl-modified NiCuMoLaAl reforming catalyst.
Phagocytic Activities of Reactive Microglia and Astrocytes Associated with Prion Diseases Are Dysregulated in Opposite Directions
Authors: Sinha Anshuman , Kushwaha Rajesh , Molesworth Kara , Mychko Olga , Makarava Natallia , Baskakov Ilia V.
Published: July 2021

Phagocytosis is one of the most important physiological functions of the glia directed at maintaining a healthy, homeostatic environment in the brain. Under a homeostatic environment, the phagocytic activities of astrocytes and microglia are tightly coordinated in time and space. In neurodegenerative diseases, both microglia and astrocytes contribute to neuroinflammation and disease pathogenesis, however, whether their phagocytic activities are up- or downregulated in reactive states is not known. To address this question, this current study isolated microglia and astrocytes from C57BL/6J mice infected with prions and tested their phagocytic activities in live-cell imaging assays that used synaptosomes and myelin debris as substrates. The phagocytic uptake by the reactive microglia was found to be significantly upregulated, whereas that of the reactive astrocytes was strongly downregulated. The up- and downregulation of phagocytosis by the two cell types were observed irrespective of whether disease-associated synaptosomes, normal synaptosomes, or myelin debris were used in the assays, indicating that dysregulations are dictated by cell reactive states, not substrates. Analysis of gene expression confirmed dysregulation of phagocytic functions in both cell types. Immunostaining of animal brains infected with prions revealed that at the terminal stage of disease, neuronal cell bodies were subject to engulfment by reactive microglia. This study suggests that imbalance in the phagocytic activities of the reactive microglia and astrocytes, which are dysregulated in opposite directions, is likely to lead to excessive microglia-mediated neuronal death on the one hand, and the inability of astrocytes to clear cell debris on the other hand, contributing to the neurotoxic effects of glia as a whole.
The Role of MSC in Wound Healing, Scarring and Regeneration
Authors: Guillamat-Prats Raquel
Published: July 2021

Tissue repair and regeneration after damage is not completely understood, and current therapies to support this process are limited. The wound healing process is associated with cell migration and proliferation, extracellular matrix remodeling, angiogenesis and re-epithelialization. In normal conditions, a wound will lead to healing, resulting in reparation of the tissue. Several risk factors, chronic inflammation, and some diseases lead to a deficient wound closure, producing a scar that can finish with a pathological fibrosis. Mesenchymal stem/stromal cells (MSCs) are widely used for their regenerative capacity and their possible therapeutically potential. Derived products of MSCs, such as exosomes or extravesicles, have shown a therapeutic potential similar to MSCs, and these cell-free products may be interesting in clinics. MSCs or their derivative products have shown paracrine beneficial effects, regulating inflammation, modifying the fibroblast activation and production of collagen and promoting neovascularization and re-epithelialization. This review describes the effects of MSCs and their derived products in each step of the wound repair process. As well, it reviews the pre-clinical and clinical use of MSCs to benefit in skin wound healing in diabetic associated wounds and in pathophysiological fibrosis.
Age-Related Differential Effects of School-Based Sitting and Movement Meditation on Creativity and Spatial Cognition: A Pilot Study
Authors: Marson Fabio , Fano Antonio De , Pellegrino Michele , Pesce Caterina , Glicksohn Joseph , Ben-Soussan Tal Dotan
Published: July 2021

Psychophysical well-being can be supported during development by the integration of extra-curricular activities in scholastic settings. These activities can be implemented in different forms, ranging from physical activities to sitting meditation practices. Considering that both such activities are thought to affect children’s psychophysical development, a movement-based meditation that combines the two approaches−in the form of a short daily activity−could represent a powerful tool to promote healthy physical and mental development. Consequently, the current pilot study aimed to examine the effect of short daily school-based sitting and movement meditation trainings on creativity and spatial cognition. Utilizing a crossover design, we evaluated their feasibility and efficacy at different ages among children (n = 50) in 5th to 8th grade. We observed that 5 weeks of daily training in sitting and movement meditation techniques improved children’s cognition differently. Specifically, younger children showed greater creativity and better spatial cognition following the movement-based meditation, while older children showed greater enhancement in these areas following sitting meditation training. This suggests that training can affect children’s cognition differently depending on their developmental stage. We discuss these results within the framework of embodied and grounded cognition theories. Information on feasibility and age-related effect sizes derived from the current study paves the way for future well-powered larger-scale efficacy studies on different forms of school-based interventions to cognitive development promotion.
Performance Evaluation and Comparison of Satellite-Derived Rainfall Datasets over the Ziway Lake Basin, Ethiopia
Authors: Hordofa Aster Tesfaye , Leta Olkeba Tolessa , Alamirew Tena , Kawo Nafyad Serre , Chukalla Abebe Demissie
Published: July 2021

Consistent time series rainfall datasets are important in performing climate trend analyses and agro-hydrological modeling. However, temporally consistent ground-based and long-term observed rainfall data are usually lacking for such analyses, especially in mountainous and developing countries. In the absence of such data, satellite-derived rainfall products, such as the Climate Hazard Infrared Precipitations with Stations (CHIRPS) and Global Precipitation Measurement Integrated Multi-SatellitE Retrieval (GPM-IMERG) can be used. However, as their performance varies from region to region, it is of interest to evaluate the accuracy of satellite-derived rainfall products at the basin scale using ground-based observations. In this study, we evaluated and demonstrated the performance of the three-run GPM-IMERG (early, late, and final) and CHIRPS rainfall datasets against the ground-based observations over the Ziway Lake Basin in Ethiopia. We performed the analysis at monthly and seasonal time scales from 2000 to 2014, using multiple statistical evaluation criteria and graphical methods. While both GPM-IMERG and CHIRPS showed good agreement with ground-observed rainfall data at monthly and seasonal time scales, the CHIRPS products slightly outperformed the GPM-IMERG products. The study thus concluded that CHIRPS or GPM-IMERG rainfall data can be used as a surrogate in the absence of ground-based observed rainfall data for monthly or seasonal agro-hydrological studies.
The Influence of the Preheating Temperature of the (−2 0 1) β-Ga2O3 Substrates on c-Plane GaN Epitaxial Growth
Authors: Lan Yu-Pin
Published: July 2021

In this paper, we demonstrate the direct epitaxial growth of c-plane GaN on a preheated (−2 0 1) β-Ga2O3 single-crystal substrate with no interlayer or pre-patterning processes by using the atmospheric pressure metalorganic chemical vapor deposition method. The results show that high-temperature preheating (>500 °C) can modify the surface morphology of the substrate so that the crystalline quality of the grown GaN layer can be improved. With higher preheated temperatures, the grown GaN layer reveals smaller FWHM (full width at half-maximum) of the X-ray rocking curve. In addition, we find that the photoluminescence spectra of the GaN layers reveal their narrowest linewidth at a preheated temperature of 800 °C. These results support improvements of crystalline quality and provide optimization of a c-GaN grown epitaxially on the preheated (−2 0 1) β-Ga2O3 substrates for further device fabrication.
Effect of Vitamin D Deficiency on COVID-19 Status: A Systematic Review
Authors: Das Pranta , Samad Nandeeta , Ahinkorah Bright Opoku , Hagan John Elvis , Peprah Prince , Mohammed Aliu , Seidu Abdul-Aziz
Published: July 2021

One major micronutrient studied for its possible protective effect against the COVID-19 disease is vitamin D. This systematic review sought to identify and synthesize available evidence to aid the understanding of the possible effect of vitamin D deficiency on COVID-19 status and health outcomes in COVID-19 patients. Three databases (PubMed, ScienceDirect, and Google Scholar) were systematically used to obtain English language journal articles published between 1 December 2019 and 3 November 2020. The search consisted of the terms (“Vitamin D,” OR “25-Hydroxyvitamin D,” OR “Low vitamin D.”) AND (“COVID-19” OR “2019-nCoV” OR “Coronavirus” OR “SARS-CoV-2”) AND (“disease severity” OR “IMV” OR “ICU admission” OR “mortality” OR “hospitalization” OR “infection”). We followed the recommended PRISMA guidelines in executing this study. After going through the screening of the articles, eleven articles were included in the review. All the included studies reported a positive association between vitamin D sufficiency and improved COVID-19 disease outcomes. On the other hand, vitamin D deficiency was associated with poor COVID-19 disease outcomes. Specifically, two studies found that vitamin D-deficient patients were more likely to die from COVID-19 compared to vitamin D-sufficient patients. Three studies showed that vitamin D-deficient people were more likely to develop severe COVID-19 disease compared to vitamin D-sufficient people. Furthermore, six studies found that vitamin D-deficient people were more likely to be COVID-19 infected compared to vitamin D-sufficient people. Findings from these studies suggest that vitamin D may serve as a mitigating effect for COVID-19 infection, severity, and mortality. The current evidence supports the recommendations for people to eat foods rich in vitamin D such as fish, red meat, liver, and egg yolks. The evidence also supports the provision of vitamin D supplements to individuals with COVID-19 disease and those at risk of COVID-19 infection in order to boost their immunity and improve health outcomes.
Antibody Response against the SARS-CoV-2 Nucleocapsid Protein and Its Subdomains—Identification of Pre-Immunization Status by Human Coronaviruses with Multipanel Nucleocapsid Fragment Immunoblotting
Authors: Pajenda Sahra , Kapps Sebastian , Reiter Thomas , Freire Raimundo , Smits Veronique A. J. , Wagner Ludwig , Gerges Daniela , Winnicki Wolfgang , Sunder-Plassmann Gere , Schmidt Alice
Published: July 2021

A novel beta coronavirus that emerged in late December 2019 triggered a global pandemic. Diagnostic methods for rapid identification of infected individuals were established in new biotechnological approaches. Vaccine production and application to individuals and measurement of SARS-CoV-2 antibodies also began. Serum samples from 240 health care workers were collected at three-month intervals over nine months. Indirect SARS-CoV-2 nucleocapsid IgG ELISA tests were used to identify humoral immune responses. All seropositive individuals and those with borderline ELISA values were tested with a specifically generated multipanel nucleocapsid fragment immunoblot. Of the 240 individuals, 24 showed seroconversion in ELISA after experiencing COVID-19. All of them showed a positive reaction against the full-length nucleocapsid protein in the immunoblot. The highest reactivity was seen either against fragment N(100–300) or in a minority against the posterior part N(200–419). In general, the staining pattern of COVID-19 patients showed four phenotypes. In contrast, three individuals classified as borderline by ELISA reacted exclusively with fragments N(1–220) and N(100–300) containing the octamer amino acid sequence FYYLGTGP, which is identical in human coronaviruses sharing this sequence with SARS-CoV-2. These represent a unique and thus fifth phenotype. This work suggests the existence of distinct phenotypic patterns of IgG production towards N-protein subdomains.
A New Viral Coinfection: SARS-CoV-2 Pneumonia and Cytomegalovirus Pneumonitis in a Renal Transplant Recipient
Authors: Shaikh Abdullah S. , Shaim Hila , Caravedo Maria A. , Ong Karen M. , Reynoso David
Published: July 2021

SARS-CoV-2 has caused a global pandemic of an acute respiratory illness known as COVID-19. Patients with solid organ transplants receiving chronic immunosuppressive therapy are at risk of severe disease caused by opportunistic pathogens, including cytomegalovirus (CMV). We present the case of a renal transplant recipient presenting with hypoxic respiratory failure because of severe COVID-19, whose course was complicated by ganciclovir-resistant CMV pneumonitis.
Variation in Alpine Plant Diversity and Soil Temperatures in Two Mountain Landscapes of South Patagonia
Authors: Lencinas María Vanessa , Soler Rosina , Cellini Juan Manuel , Bahamonde Héctor , Pérez Flores Magalí , Monelos Lucas , Martínez Pastur Guillermo José , Peri Pablo Luis
Published: July 2021

Alpine environments and their temporal changes are rarely studied at high latitudes in the southern hemisphere. We analyzed alpine plants, soil temperatures, and growing-season length in mountains of two landscapes of South Patagonia (46° to 56° SL): three summits (814–1085 m a.s.l) surrounded by foothill grasslands in Santa Cruz province (SC), and four summits (634–864 m a.s.l.) in sub-Antarctic forests of Tierra del Fuego province (TF). Sampling followed the protocolized methodology of the Global Observational Research Initiative in Alpine Environments (GLORIA). Factors were topography (elevation and cardinal aspect) and time (baseline vs. re-sampling for plants, five annual periods for temperatures), assessed by univariate and multivariate tests. Plant composition reflected the lowland surrounding landscapes, with only 9 mountain species on 52 totals in SC and 3 on 30 in TF. Richness was higher in re-sampling than baseline, being assemblages more influenced by aspect than elevation. Mean annual soil temperature and growing-season length, which varied with topography, were related to the Multivariate El Niño Southern Oscillation Index (MEI) but did not show clear warming trends over time. We highlight the importance of long-term studies in mountainous regions of extreme southern latitudes, where factors other than warming (e.g., extreme climate events) explain variations.
Ocean Acidification and Mollusc Settlement in Posidonia oceanica Meadows: Does the Seagrass Buffer Lower pH Effects at CO2 Vents?
Authors: Barruffo Alessandra , Ciaralli Laura , Ardizzone Giandomenico , Gambi Maria Cristina , Casoli Edoardo
Published: July 2021

Ocean acidification has been broadly recognised to have effects on the structure and functioning of marine benthic communities. The selection of tolerant or vulnerable species can also occur during settlement phases, especially for calcifying organisms which are more vulnerable to low pH–high pCO2 conditions. Here, we use three natural CO2 vents (Castello Aragonese north and south sides, and Vullatura, Ischia, Italy) to assess the effect of a decrease of seawater pH on the settlement of Mollusca in Posidonia oceanica meadows, and to test the possible buffering effect provided by the seagrass. Artificial collectors were installed and collected after 33 days, during April–May 2019, in three different microhabitats within the meadow (canopy, bottom/rhizome level, and dead matte without plant cover), following a pH decreasing gradient from an extremely low pH zone (pH < 7.4), to ambient pH conditions (pH = 8.10). A total of 4659 specimens of Mollusca, belonging to 57 different taxa, were collected. The number of taxa was lower in low and extremely low pH conditions. Reduced mollusc assemblages were reported at the acidified stations, where few taxa accounted for a high number of individuals. Multivariate analyses revealed significant differences in mollusc assemblages among pH conditions, microhabitat, and the interaction of these two factors. Acanthocardia echinata, Alvania lineata, Alvania sp. juv, Eatonina fulgida, Hiatella arctica, Mytilys galloprovincialis, Musculus subpictus, Phorcus sp. juv, and Rissoa variabilis were the species mostly found in low and extremely low pH stations, and were all relatively robust to acidified conditions. Samples placed on the dead matte under acidified conditions at the Vullatura vent showed lower diversity and abundances if compared to canopy and bottom/rhizome samples, suggesting a possible buffering role of the Posidonia on mollusc settlement. Our study provides new evidence of shifts in marine benthic communities due to ocean acidification and evidence of how P. oceanica meadows could mitigate its effects on associated biota in light of future climate change.
Public Health and Disability: A Real-Life Example of the Importance of Keeping Up the Good Work
Authors: Alriksson-Schmidt Ann I. , Hägglund Gunnar
Published: July 2021

Secondary and tertiary prevention strategies are essential in targeting those with disabilities to improve their health and well-being. In Sweden, over 95% of all children with cerebral palsy (CP) participate in a follow-up program in which one of the goals is to prevent hip dislocations. To demonstrate the importance of maintaining timely, systematic, preventive, work overtime, we reviewed the incidence of hip dislocations from 2010 to 2019 and the number of children who underwent different types of hip surgeries in Sweden. Leading to 2015, the number of hip dislocations reduced from 8.8% before the introduction of the program to 0.3–0.4%, followed by a gradual increase to 0.8% in 2019. The proportion of children who underwent adductor–psoas lengthening as their primary preventative surgery decreased from 65% in 2010 to 45% in 2019, with a corresponding increase of children undergoing femoral osteotomy, indicating that more children underwent surgery at a later stage. One of the likely reasons for the increase in hip dislocations includes longer waiting periods before surgery, possibly due to a shortage of nurses. At least seven of the 29 children with dislocated hips waited more than one year for preventative surgery and developed a hip dislocation during this period. It is also possible that the increased number of children with hip dislocations may be associated with the fact that more children with CP have immigrated to Sweden in recent years without receiving corresponding compensation in healthcare resources. The results highlight the importance of constantly monitoring follow-up programs to swiftly notice alarming trends that require immediate action.
A Hybrid Approach for Autonomous Collision-Free UAV Navigation in 3D Partially Unknown Dynamic Environments
Authors: Elmokadem Taha , Savkin Andrey V.
Published: July 2021

In the past decades, unmanned aerial vehicles (UAVs) have emerged in a wide range of applications. Owing to the advances in UAV technologies related to sensing, computing, power, etc., it has become possible to carry out missions autonomously. A key component to achieving this goal is the development of safe navigation methods, which is the main focus of this work. A hybrid navigation approach is proposed to allow safe autonomous operations in three-dimensional (3D) partially unknown and dynamic environments. This method combines a global path planning algorithm, namely RRT-Connect, with a reactive control law based on sliding mode control to provide quick reflex-like reactions to newly detected obstacles. The performance of the suggested approach is validated using simulations.
The Violation of Bell-CHSH Inequalities Leads to Different Conclusions Depending on the Description Used
Authors: Solis-Labastida Aldo F. G. , Gastelum Melina , Hirsch Jorge G.
Published: July 2021

Since the experimental observation of the violation of the Bell-CHSH inequalities, much has been said about the non-local and contextual character of the underlying system. However, the hypothesis from which Bell’s inequalities are derived differ according to the probability space used to write them. The violation of Bell’s inequalities can, alternatively, be explained by assuming that the hidden variables do not exist at all, that they exist but their values cannot be simultaneously assigned, that the values can be assigned but joint probabilities cannot be properly defined, or that averages taken in different contexts cannot be combined. All of the above are valid options, selected by different communities to provide support to their particular research program.
Transitioning of Steel Producers to the Steelworks 4.0—Literature Review with Case Studies
Authors: Gajdzik Bożena , Wolniak Radosław
Published: July 2021

The publication presents a picture of modern steelworks that is evolving from steelworks 3.0 to steelworks 4.0. The paper was created on the basis of secondary sources of information (desk research). The entire publication concerns the emerging opportunities for the development of the steel producers to Industry 4.0 and the changes already implemented in the steel plants. The collected information shows the support environment for changes in the steel sector (EU programs), the levels of evolution of steel mills, along with the areas of change in the steel industry and implemented investment projects. The work consists of a theoretical part based on a literature review and a practical part based on case studies. The work ends with a discussion in which the staged and segmented nature of the changes introduced in the analyzed sector is emphasized. Based on the three case studies described in the paper, a comparative analysis was conducted between them. When we tried to compare methods used in the three analyzed steel producers (capital groups): ArcelorMittal, Thyssenkrupp, and Tata Steel Group, it can be seen that in all organizations, the main problem connected with steelworks 4.0 transition is the digitalization of all processes within an organization and in the entire supply chain. This is realized using various tools and methods but they are concentrated on using technologies and methods such as artificial intelligence, drones, virtual reality, full automatization, and industrial robots. The effects are connected to better relations with customers, which leads to an increase in customer satisfaction and the organizations’ profit. The steel industry will undergo further strong changes, bringing it closer to Industry 4.0 because it occupies an important place in the economies of many countries due to the strong dependence of steel producers on the markets of the recipients (steel consumers). Steel is the basic material needed to make many products, and its properties have been valued for centuries. In addition, steel mills with positive economic, social, and environmental aspects are part of the concept of sustainability for industries and economies.
The Impact of High-Energy Mining-Induced Tremor in a Fault Zone on Damage to Buildings
Authors: Pilecka Elżbieta , Stec Krystyna , Chodacki Jacek , Pilecki Zenon , Szermer-Zaucha Renata , Krawiec Krzysztof
Published: July 2021

Seismic energy propagation from the hypocentre of mining-induced tremors usually causes an uneven distribution of the peak ground velocity PGVHmax in tectonically complicated structures, and consequently, an uneven distribution of damage to buildings located on the ground surface. This study aimed to estimate the impact of high-energy mining-induced tremors in fault zones on damage to buildings. In the study, we describe a case of one of the highest-energy mining-induced tremors E = 4.0 · 108 J (local magnitude ML = 3.6) that occurred in the Upper Silesian Coal Basin (USCB), Poland. The hypocentre of the tremor was most probably located in the Barbara fault zone, one of the larger faults in that western part of the USCB. Numerous damaged buildings on the terrain surface were registered, both in the epicentral zone and at a greater distance from the epicentre, mostly from the southern side of the Barbara fault zone. We calculated that the tremor was characterised by a normal slip mechanism associated with the same kind of fault as the Barbara fault. The azimuth of the nodal planes was similar to the west-east direction, which is consistent with the azimuth of the Barbara fault. From the focal mechanism, the greatest propagation of seismic energy occurred in south and west-east directions from the tremor hypocentre towards the surface. It was found that from the northern side of the hanging wall of the Barbara fault, there were 14 instances of damage (19%), and in the southern part of a hanging wall, there were 58 (81%). Therefore, the directionality of seismic energy propagation is aligned with the focal mechanism acting in the Barbara fault. It has also been concluded that a width of the zone of up to about 1200 m along the Barbara fault is the most threatening on the basis of registered building damage in the geological conditions of USCB. The study has shown that in assessing the impact of mining-induced tremors on buildings and the environment, the disturbance of seismic energy propagation by larger faults should be considered.
The Effect of the Thermal Mass of the Building Envelope on Summer Overheating of Dwellings in a Temperate Climate
Authors: Kuczyński Tadeusz , Staszczuk Anna , Ziembicki Piotr , Paluszak Anna
Published: July 2021

The main objective of this paper is to demonstrate the effectiveness of increasing the thermal capacity of a residential building by using traditional building materials to reduce the risk of its excessive overheating during intense heat waves in a temperate climate. An additional objective is to show that the use of this single passive measure significantly reduces the risk of overheating in daytime rooms, but also, though to a much lesser extent, in bedrooms. Increasing the thermal mass of the room from light to a medium heavy reduced the average maximum daily temperature by 2.2K during the first heat wave and by 2.6K during the other two heat waves. The use of very heavy construction further reduced the average maximum temperature for the heat waves analyzed by 1.4K, 1.2K and 1.7K, respectively, giving a total possible reduction in maximum daily temperatures in the range of 3.6 °C, 3.8 °C and 4.3 °C. A discussion of the influence of occupant behavior on the use of night ventilation and external blinds was carried out, finding a significant effect on the effectiveness of the use of both methods. The results of the study suggest that in temperate European countries, preserving residential construction methods with heavy envelopes and partitions could significantly reduce the risk of overheating in residential buildings over the next few decades, without the need for night ventilation or external blinds, whose effectiveness is highly dependent on individual occupant behavior.
Application of Dynamic Fault Tree Analysis to Prioritize Electric Power Systems in Nuclear Power Plants
Authors: Baek Sejin , Heo Gyunyoung
Published: July 2021

Because the scope of risk assessments at nuclear power plants (NPPs) is being extended both spatially and temporally, conventional, or static fault trees might not be able to express failure mechanisms, or they could be unnecessarily conservative in their expression. Therefore, realistic assessment techniques are needed to adequately capture accident scenarios. In multi-unit probabilistic safety assessment (PSA), fault trees naturally become more complex as the number of units increases. In particular, when considering a shared facility between units of the electric power system (EPS), static fault trees (SFTs) that prioritize a specific unit are limited in implementing interactions between units. However, dynamic fault trees (DFTs) can be available without this limitation by using dynamic gates. Therefore, this study implements SFTs and DFTs for an EPS of two virtual NPPs and compares their results. In addition, to demonstrate the dynamic characteristics of the shared facilities, a station blackout (SBO), which causes the power system to lose its function, is assumed—especially with an inter-unit shared facility, AAC DG (Alternate AC Diesel Generator). To properly model the dynamic characteristics of the shared EPS in DFTs, a modified dynamic gate and algorithm are introduced, and a Monte Carlo simulation is adopted to quantify the DFT models. Through the analysis of the DFT, it is possible to confirm the actual connection priority of AAC DG according to the situation of units in a site. In addition, it is confirmed that some conservative results presented by the SFT can be evaluated from a more realistic perspective by reflecting this.