bluuebunny/crossref_metadata_2025 huggingface dataset:
ON THE MODELS OF SEGREGATION DISTORTION IN DROSOPHILA MELANOGASTER
Authors: Martin Diana W , Hiraizumi Yuichiro
Published: April 2021

The Segregation Distorter system of Drosophila melanogaster consists of two major elements, Sd and Rsp. There are two allelic alternatives of Rsp—sensitive (Rsp8) and insensitive (Rspi); a chromosome carrying Rspi is not distorted. According to the model proposed by HARTL (1973), these two elements interact to cause segregation distortion. For a sperm to complete the maturation process, it is assumed that the Rsp locus has to be complexed with the product of the Sd locus. This product is assumed to be a multimetric regulatory protein. Three kinds of regulatory multimers may be distinguished: Sd+/Sd+, which is assumed to complex with both Rsp8 and Rspi; Sd+/Sd heteromultimers, which complex preferentially with Rspi; and Sd/Sd hmomultimers, which complex with neither Rsp8 nor Rspi. Most of the regulatory protein in the Sd+/Sd heterozygous male is assumed to be the Sd+/Sd heteromultimer. ——Some modifications of HARTL'S model were made by GANETZEY (1977). Rather than the binding of a product of Sd at the Rsp locus being a necessary condition for normal spermigenesis, this binding causes sperm dysfunction. It is assumed that the product of Sd complexes more readily with Rsp8 than with Rspi and that the amount of Sd product is limited with respect to the number of binding sites available. No function is ascribed to the Sd+ locus. In order to explain reduced male fertility of some genotypes, GANETZKY further assumes that the Sd product, when not competed for by an Rsp8 locus, can bind to an Rspi locus.——Two consequences of these models were criticically examined: according to these models (1) an Sd Rsp8/Sd+ RspS male should not show any segregation distortion, and (2) an Sd Rsps/Sd Rsp8 male should show much reduced fertility, if not complete sterility.—The results of the present study bear on these two points. (1) Rsp8 locus seems to consist of multiple alleles, each having a different degree of ability to interact with the product of the Sd locus. An Sd Rsp8/Sd+RspS male shows a certain degree of segregation distortion when the two RspS alleles are different, but it shows a normal Mendelian segregation ratio when the Rsp8 alleles are homozygous. The first prediction of the models is supported by actual observation when the two Rsps alleles are the same. (2) There is a suggestion of slight reduction in fertility, but generally Sd Rsp8/Sd Rsp8 males are quite fertile. Thus, the second prediction is not supported by actual observation. The mechanism of segregation distortion is still open for future studies.
A MODEL OF THE NEGATIVE CORRELATION BETWEEN MALE RECOMBINATION AND TRANSMISSION FREQUENCY IN DROSOPHILA MELANOGASTER
Authors: Hiraizumi Yuichiro
Published: April 2021

A model is proposed to account for the phenomenon of negative correlation between male recombination (θ) and transmission frequency (k) in Drosophila melanogaster.

The model assumes that, in some stage or stages of development, the male recombination elements cause a particular event that does not occur in normal males and that this event, in turn, induces with certain probabilities male recombination and/or sperm dysfunction.

The regression equations of θ on k predicted by the model were compared with those actually observed. There was generally excellent agreement between them.
THE GENETICS OF DROSOPHILA SUBOBSCURA POPULATIONS. IX. STUDIES ON LINKAGE DISEQUILIBRIUM IN FOUR NATURAL POPULATIONS
Authors: Loukas M , Krimbas C B , Vergini Y
Published: April 2021

Gametic frequencies were obtained in four natural populations of D. subobscura by extracting wild chromosomes and subsequently analyzing them for inversions and allozymes. The genes Lap and Pept-1, both located within the same inversions of chromosome 0, were found in striking nonrandom associations with them of the same kind and degree in all populations studied. On the contrary, the gene Acph, also located within the previously mentioned inversions, was found in linkage disequilibrium with them only in two populations and of opposite directions. This is also the case for the genes Est-9 and Hk, both located within chromosome E inversions. While the gene Est-9 was in strong linkage disequilibrium with the inversions, of the same kind and degree in all populations studied, Hk was found to be in linkage equilibrium.

Allele frequencies for the 29 genes studied do not show geographical variation except for the genes Lap, Pept-1 and Est-9, the ones found in linkage disequilibria with the geographically varying gene arrangements. Although mechanical or historical explanations for these equilibria cannot be ruled out, these data cannot be explained satisfactorily by the "middle gene explanation," which states that loci displaying such linkage disequilibria are the ones located near the break points of inversions, while the ones displaying linkage equilibria with them are located in the middle of them.

There is no evidence for consistent linkage disequilibria between pairs of loci, except for the closely linked genes of the complex locus, Est-9. This would imply, if it is not a peculiarity of the Est-9 complex, that the linkage disequilibria aye found only between very closely linked loci or that, far less closely linked genes, the associations are too weak to be detected by the usual samples sizes.
PROTOPLASMIC INCOMPATIBILITY IN PODOSPORA ANSERINA: A POSSIBLE ROLE FOR ITS ASSOCIATED PROTEOLYTIC ACTIVITY
Authors: Labarere Jacques , Bernet Jean
Published: April 2021

A mutation (modD) was selected in a gene involved in the control of protoplasmic incompatibility. Previous results (LABARERE and BERNET 1979) showed that modD decreased the density of protoperithecia and caused a defect in ascospore germination. In addition, modD has a third defect: when modD stationary cells were isolated in order to obtain further development, renewal of growth rarely ensued. Instead, the modD cells lysed or produced microthalli from which normal growth never occurred. These defects were suppressed by β-phenyl pyruvic acid, a protease inhibitor, and by the presence of a mutation (modC) that suppresses the proteases associated with protoplasmic incompatibility. The stationary wild-type cells' regeneration was inhibited by β-phenyl pyruvic acid at levels that maintained modD cells' regeneration. These results suggest a biological role for the proteases associated with protoplasmic incompatibility.
UNIDIRECTIONAL GENE CONVERSION ASSOCIATED WITH TWO INSERTIONS IN NEUROSPORA CRASSA MITOCHONDRIAL DNA
Authors: Mannella Carmen A , Lambowitz Alan M
Published: April 2021

The mitochondrial phenotype of [poky] and other extranuclear Neurospora mutants is known to predominate over that of wild type in hetero-plasmons. In the present work, we have investigated the interaction between wild-type and [poky] mtDNAs using as many as four physical markers to distinguish the two types of mtDNAs. Two insertions, one of 1200 bp in Eco RI-5 and the other 50 bp in Eco RI-9, are identified as sites of high frequency, unidirectional gene conversion leading to their spread through mtDNA populations in heteroplasmons. However, the transmission of the [poky] mutation does not appear to be correlated with the transmission of either of these insertions or of other physical markers. The possibility that other loci of nonreciprocal recombination might be responsible for the "dominance" of Neurospora extranuclear mutants is discussed.
GENETIC MODULATION OF RNA METABOLISM IN DROSOPHILA. IV. MEASUREMENT OF RATES OF RNA SYNTHESIS BY DENSITY LABELING
Authors: Strausbaugh Linda D , Clark Stephen H , Grainger Robert M
Published: April 2021

Accumulation of RNA was measured in adult males of two genotypes: car bb/Ybb- and car bb/YbbSuvar-5. The two genotypes have similar amounts of rDNA, which is reduced in comparison to wild type (CLARK, STRAUSBAUGH and KIErm 1977). Although genotypically bobbed, car bb/YbbSuVar-5 flies have a wild-type phenotype; car bb/Ybb- flies are both phenotypically and genotypically bobbed (CLARK, STRAUSBAUGH and KIEFER 1977). The wild-type phenotype observed in the car bb/YbbsuVar-5 flies is thought to be the result of an increased rate of rRNA synthesis due ot the presence of the YbbSuVar-5 chromosome (SHERMOEN and KIFFER 1975; CLARK, STRAUSBAUGH and KIEFER 1977; CLARK and KIEFER 1977). To further define this phenomenon, the absolute accumulation of RNA was measured in the two genotypes, using density labeling methods. The accumulation of RNA is 1.4 to 1.8 times higher in car bb/YbbSuVar-5 flies than in car bb/Ybb- flies, demonstrating that there is genetic regulation of synthesis in this genotype. The use of density-labeled nucleosides has clearly shown that there is no difference in precusor pool sizes or use between the two genotypes studied.
IDENTIFICATION OF AN R-LOCUS REGION THAT CONTROLS THE TISSUE SPECIFICITY OF ANTHOCYANIN FORMATION IN MAIZE
Authors: Dooner Hugo K
Published: April 2021

The R locus in maize controls the tissue specificity of anthocyanin formation. Recombination between two different R-locus alleles with unique pigmentation domains has resulted in the generation of a nonparental allelic type that displays a third kind of tissue specificity. These novel changes in tissue specificity occurred in very low frequency (1 x 10-5). In light of this finding and of recent results concerning the regulation by R of the enzyme UDPG: flavonoid 3-O-glucosyltransferase, specified by the Bz locus, a model dealing with the genetic control of tissue-specific functions in multicellular organisms is proposed.
ISOZYME POLYMORPHISM IN NATURAL POPULATIONS OF A LIVERWORT, PLAGIOCHILA ASPLENIOIDES
Authors: Krzakowa M , Szweykowski J
Published: April 2021

Assays of five Polish populations of Plagiochila asplenioides (Hepaticae, Plagiochilaceae) for peroxidase isozymes revealed three bands differing in electrophoretic mobility. The phenotypes observed appear to be governed by three loci; each locus has two alleles, one of which produces a band and the other no band, i.e., the second allele is silent, or null. Marked variability in enzyme phenotypes was found both within and between populations. The evolutionary implications of this variability are discussed in terms of the persistent haplophase and ephemeral diplophase of this organism.
CYTOGENETIC ANALYSIS OF CHROMOSOME 3 IN DROSOPHILA MELANOGASTER: THE HOMOEOTIC GENE COMPLEX IN POLYTENE CHROMOSOME INTERVAL 84A-B
Authors: Kaufman Thomas C , Lewis Ricki , Wakimoto Barbara
Published: April 2021

Cytogenetic evidence is presented demonstrating that the 84A-B interval in the proximal portion of the right arm of chromosome 3 is the residence of a homoeotic gene complex similar to the bithorax locus. This complex, originally defined by the Antennapedia (A n t p) mutation, controls segmentation in the anterior portion of the organism. Different lesions within this complex homoeotically transform portions OI the prothorax, proboscis, antenna and eye and present clear analogies to similar lesions within the bithorax locus.
THE STEPWISE MUTATION MODEL: AN EXPERIMENTAL EVALUATION UTILIZING HEMOGLOBIN VARIANTS
Authors: Fuerst Paul A , Ferrell Robert E
Published: April 2021

The stepwise mutation model of OHTA and KIMURA (1973) was proposed to explain patterns of genetic variability revealed by means of electrophoresis. The assumption that electrophoretic mobility was principally determined by unit changes in net molecular charge has been criticized by JOHNSON (1974, 1977). This assumption has been tested directly using hemoglobin. Twenty-seven human hemoglobin variants with known amino acid substitutions, and 26 nonhuman hemoglobins with known sequences were studied by starch gel electrophoresis. Of these hemoglobin% 60 to 70% had electrophoretic mobilities that could be predicted solely on the basis of net charge calculated from the amino acid composition alone, ignoring tertiary structure. Only four hemoglobins showed a mobility that was clearly different from an expected mobility calculated using only the net charge of the molecule. For the remaining 30% of hemoglobins studied, mobility was determined by a combination. of net charge and other unidentified components, probably reflecting changes in ionization of some amino acid residues as a result of small alterations in tertiary structure due to the amino acid substitution in the variant. For the nonhuman hemoglobins, the deviation of a sample from its expected mobility increased with increasing amino acid divergence from human hemoglobin A.—It is concluded that the net electrostatic charge of a molecule is the principal determinant of electrophoretic mobility under the conditions studied. However, because of the significant deviation from strict stepwise mobility detected for 30 to 40% of the variants studied, it is further concluded that the infinite-allele model of KIMURA and CROW (1964) or a "mixed model" such as that proposed by LI (1976) may be more appropriate than the stepwise mutation model for the analysis of much of the available electrophoretic data from natural populations.
MATING-TYPE INHERITANCE AND MATURITY TIMES IN CROSSES BETWEEN SUBSPECIES OF TETRAHYMENA PIGMENTOSA
Authors: Simon Ellen M
Published: April 2021

Subspecies 6 and 8 of T . pigmentosa (formerly syngens 6 and 8 of T . pyriformis) share a mating-type system controlled by three alleles with "peck-order'' dominance at a single locus. The system is apparently closed and limited to three mating types that are homologous, but not identical, in the subspecies. These relationships are reflected in new mating-type designations. —The viability in some intersyngenic crosses is excellent, and the inheritance of major mating types in first-generation hybrids and their progeny follows the pattern of subspecies 8.—The period of immaturity is shorter than that previously reported for subspecies 8, with 50% of the subclones maturing between 46 and 100 fissions after conjugation. Maturity curves are generally sigmoid, but some are apparently biphasic. The onset of maturity in triplicate sublines from the same synclone is usually highly correlated.
GENETIC MAPPING AND SOME CHARACTERIZATION OF THE rnpA49 MUTATION OF ESCHERICHIA COLI THAT AFFECTS THE RNA-PROCESSING ENZYME RIBONUCLEASE P
Authors: Apirion David
Published: April 2021

A mutant defective in the enzyme RNase P was isolated by P. SCHEDL and P. PRIMAKOFF (1973). The mutation rnpA49 found in this strain, which confers temperature sensitivity on carrier strains, was mapped by conjugation and transduction experiments and located around minute 82 of the E . coli map, with the suggested order rnpA bglB phoS rbsP ilv. As expected, the rnpA49 mutation is recessive. Even though this mutation is conditional, it is manifested at temperatures at which the carrier strains can grow.
GENETIC EVIDENCE FOR INTERACTION BETWEEN NONHOMOLOGOUS PROTEINS IN YEAST AND A CASE OF SUPPRESSION AT THE HIS1 LOCUS
Authors: Snow Richard
Published: April 2021

The HIS1 and THR4 loci are the structural genes for phosphoribosyl-ATP pyrophosphorylase and threonine synthetase, respectively. The allele his1-IS has no enzyme activity at 30", but does have activity at 15" provided the cell contains the wild-type THR4 allele or a suppressing allele at another locus, designated SUP(his1-1S). Under these conditions, cells with the hisl-IS mutation are capable of growth on minimal medium at 15". Three kinds of reversions of a hisl-IS thr4 sup(his1-IS) strain to histidine prototrophy have been obtained: (1) his1-IS locus reversions to HIS1 that restore growth without added histidine at 30", (2)  thr4 reversions to THR4 that simultaneously eliminate the requirement for threonine and restore the low-temperature effect on the his1-IS allele, and (3)mutations from sup to SUP. The SUP allele is not an ochre suppressor, and it is not linked to either HISI, THR4 or a centromere. It may represent a missense suppressor. I t is proposed that the effect ofTHR4 is caused by aggregation of the wild-type threonine synthetase with defective his1-IS monomers, causing a favorable conformational change in the histidine protein that restores limited enzymatic activity. This can be regarded as a case of complementation between nonhomologous proteins.
PROPAGATION OF CORTICAL DIFFERENCES IN TETRAHYMENA
Authors: Frankel Joseph
Published: April 2021

Progeny clones were derived froin crosses arranged so that the number of ciliary meridians (corticotype) was unusually high in one partner, and normal in the other. An analysis of the propagation of corticotypes during maintenance of these clones for up to 1,000 fissions indicated that corticotypos above 21 undergo a rapid downward shift, while corticotypes in the range of 18 to 21 change slowly. Although these observations are consistent with NANNEY'Sea rlier deduction of a "stability center" at corticotype 19, there appears to be little if any difference in the stability of perpetuation of corticotypes 18, 19 and 20. Within this "stability range," the inertia of maintenance of pre-existing corticotypes is sufficiently strong that sister clones derived from an exconjugant pair can remain different for 1,000 fissions. These findings are consistent with observations made earlier, and those in the present study, indicating that cells in stock cultures express a substantial range of corticotypes even when maintained with frequent transfer. The results suggest that mechanisms of spatially ordered structural assembly within the cell can show sufficient fidelity to allow long-term vegetative perpetuation of phenotypic differences without artificial selection.
ERRORS IN THE ELIMINATION OF X CHROMOSOMES IN SCIARA OCELLARIS
Authors: Mori Lyria , Perondini A L P
Published: April 2021

It was previously assumed that the X-linked recessive mutation, sepia, induced errors in X-chromosome elimination during early embryogenesis of Sciam ocellaris. The results obtained in the present analysis corroborate this assumption and permit a further classification of the type of error this mutation induces. Among 85,244individuals analyzed, three kinds of aberrant flies were identified: mosaics (0.01 %), gynandromorphs (0.42%)and phenotypically exceptional individuals (0.25%).The origin ofthese abnormal flies could be ascribed to errors in selective elimination of X chromosomes that occur in male meiosis or during the early cleavages of the zygote nuclei. This last kind of error could be classified into three types: (a) error in number, (b) error in type, and (c) error in number and type of X chromosome eliminated. Evidence is provided indicating that sepia has no direct effect on the X chromosome; it has a maternal influence and exerts its effect only in the heterozygous condition.
MUTANTS SHOWING HETEROTHALLISM FROM A HOMOTHALLIC STRAIN OF SACCHAROMYCES CEREVISIAE
Authors: Oshima Takehiro , Takano Isamu
Published: April 2021

Mutants defective in mating-type conversion were isolated from ascospores of a perfect homothallism strain having the HO HMRa HMLα genotype. Eighty mutants, including 11 temperature-dependent mutants showing a or α mating potency, were isolated from 10,050 colonies derived from spores mutagenized with ethyl methanesulfonate. Of the 80 mutants, 48 were tested by crossing with an Ho HMRa HMLα heterothallic and an HO HMRa HMLα homothallic strain as standards. The results allowed their division into seven classes. Mutants in the first class were due to mutation of HO to an ho allele. The second class of mutants, csm, lacked the HO function as the result of a mutation unlinked with the HO locus. Mutants in the third and fourth classes were defective in HMRa and HMLα gene functions, respectively. Mutants in the fifth and sixth classes were due to a mutation at the mating-type locus showing iwensitivity to the function of the homothallic genes. Mutants in the seventh class lacked the expression of mating type as the result of a mutation unlinked to the mating-type locus. All the temperature-dependent mutants lost the mating potency at permissive temperature (25°). One belonging to the last class of mutants was inferred to be a mutation at a locus necessary for the expression of a mating type at restricted temperature (35°).
ALTERNATIVE PHENOTYPIC STATES IN GENOMICALLY IDENTICAL CELLS: INTERSTOCK GENETICS OF A TRICHOCYST PHENOTYPE IN PARAMECIUM TETRAURELIA
Authors: Nyberg Dennis
Published: April 2021

The trichocysts of most wild stocks of Paramecium tetraurelia discharge en masse in response to picric acid. In most nonresponding wild stocks, the defective phenotype is simply determined by a single recessive gene difference from the standard wild type, stock 51. However, two wild stocks, 146 and 148, which are completely homozygous at all loci, eqress either a nondischarge, ND, or discharge, DI, phenotype. In stock 146, both ND and DI sublines generally reproduce true to type, but observed changes are highly biased. Changes from ND to DI occur more than ten times as often as changes from DI to ND. After conjugation between ND and DI cells, genomically identical exconjugant lines from the ND parent may be either ND or DI, while thme from the DI parent invariably remain D1.—Interstock crosses between stocks 146 and 51 indicate that stock 146 possesses a recessive gene, nd146, which, when hommygous in stock 51 background, produces a distinct nondischarge phenotype, KO. Crosses between stock 146 and KO phenotype nd146 homozygotes in stock 51 background demonstrate that stock 146 possesses a dominant gene, M-ndl46, which modifies the defect of nd146 homozygotes, resulting in either the ND or DI phenotype. The two loci, M-ndl46 and nd146, are linked and estimated to be 5.3 centiMorgans apart. Stock 148 has the same alleles as stock 146 at these loci.—Presumably M-nd146 is involved in the dual phenotypic states in stock 146, but M-ndl46 nd146 homozygotes backcrossed into stock 51 are invariably discharging. The possibility that the original ND state is independent of these genes is discussed and is regarded as unlikely. The phenotypic and genetic relationship discovered in these stocks should remind population biologists that phenotypic and genotypic variability do not always have a simple relationship. The nature and frequency of epistasis in the highly inbreeding P . teiraurelia are reviewed.
INHERITANCE OF A PAROTID SECRETORY PROTEIN IN MICE AND ITS USE IN DETERMINING SALIVARY AMYLASE QUANTITATIVE VARIANTS
Authors: Owerbach David , Hjorth J Peter
Published: April 2021

Among inbred strains of mice, a major protein, PSP, produced and secreted by the parotid glands, shows variation in electrophoretic mobility and in the peptides produced by cyanogen bromide treatment. This variation is inherited as a single Mendelian factor with two alleles showing co-dominant expression. In genetic crosses, it segregates independently from the amylase complex and is closely linked to the agouti locus on chromosome 2. The protein ratios between amylase and PSP in saliva, obtained by scanning of electrophoretic gel separations, were found to reflect genetic differences in salivary amylase production in strains YBR/Cv and C3H/As.
VARIATION IN ACTIVITIES OF AMYLASE ALLOZYMES ASSOCIATED WITH CHROMOSOME INVERSIONS IN DROSOPHILA PSEUDOOBSCURA, D. PERSIMILIS AND D. MIRANDA
Authors: Norman R A , Prakash Satya
Published: April 2021

Different electrophoretic alleles of amylase show associations with particular chromosome 3 inversions in D. pseudoobscura and D. persimilis. Relative adult amylase activities were compared in 37, 37and 10 strains of D. pseudoobscura, D. persimilis and D. miranda, respectively. Strains carrying the same electrophoretic allele were compared by crossing these lines individually to a reference strain carrying a different electrophoretic mobility allele. This procedure allows comparisons among species, inversions, electromorphs and strains for genetic variation in amylase activity. F2analysis established that the activity variation co-segregates with the structural amylase locus. This type of variation could be due to either structural gene differences or differences in closely linked, cis-acting regulatory regions. Variation has been detected among and within electrophoretic mobility classes. Moreover, this variation is clearly nonrandom and reveals more of the genetic structure associated with the chromosomal inversion phylogeny of D. pseudoobscura and D. persimilis. —Some of the findings are: (1) Similar electromorphs in D. pseudoobscura and D. persimilis usually show different activities. These species show nearly complete differentiation of amylase alleles, based on activities. (2) D. persimilis has the broadest range of variation in amylase activity, about four-fold between the highest and lowest alleles. D. pseudoobscura and D. miranda are also polymorphic for activity, but have more constrained ranges of variation. D. miranda alleles show on the average about four times the activity of D. pseudoobscura alleles. (3) Some association of electrophoretic mobility and activity has been found. Alleles 1.09 of D. persimilis, as well as 1.43 and 1.55 of D. miranda, have relatively high activity. It may be that these high activity alleles are part of an adaptation to cooler habitats. (4)Within electrophoretic classes, associations of activities with inversions have been found. These are especially strong in D. persimilis. The 1.00 alleles in the ST, KL,MD and WT inversions, the 0.92 allele in the ST and MD inversions and the 1.09 allele in the WT and KLinversions have levels of activities that depend upon the arrangement in which they are located. These results demonstrate that suppression of recombination in inversion heterokaryotypes can result inextensive genic divergence between inversions.
CHROMOSOME STUDIES IN WILD POPULATIONS OF DROSOPHILA MELANOGASTER. II. RELATIONSHIP OF INVERSION FREQUENCIES TO LATITUDE, SEASON, WING-LOADING AND FLIGHT ACTIVITY
Authors: Stalker Harrison D
Published: April 2021

In the midwestern and eastern U.S. populations of Drosophila melanogaster, the Standard gene arrangements show higher frequencies in the north than in the south. In a Missouri population, and to a lesser extent in a south Texas population, the frequencies of Standard chromosomes regularly rise during the cold season and drop during the warm season, thus paralleling the north-south frequency differences. In the Missouri population in 1976 and 1978, wild males were tested far their ability to fly to bait at different ambient temperatures. In both years, males flying in nature in the temperature range of 13° to 15° showed significantly higher frequencies of Standard chromosomes than did those flying in the 16° to 28° range. Wild males flying at 13° to 15° also have different tharax/wing proportions and significantly lower wingloading indices than do those flying at 16° to 28°. Moreover, wild flies homozygous Standard in 2R and/or 3R have significantly lower wing-loading indices than flies carrying inversions in these arms. Thus, wild flies with high frequencies of Standard chromosommes are karyotypically northern, are selectively favored during the cold season, have a relatively low wing-load and are most capable of flying at critically low ambient temperatures.—In summary, in Missouri, presence or absence of the common cosmopolitan inversions is an important factor in low temperature adaptation, and at least part of the adaptive mechanism involves control of thorax/wing proportions and thus control of wing-loading.
THE SELECTION OF AMBER MUTATIONS IN GENES REQUIRED FOR COMPLETION OF START, THE CONTROLLING EVENT OF THE CELL DIVISION CYCLE OF S. CEREVISIAE
Authors: Reed Steven I
Published: April 2021

Using a modification of a procedure developed for the isolation of temperature-sensitive mutants defective in the start event of cell division, amber mutations were obtained for two Class-I start genes, cdc28 and cdc37. Genetic analysis demonstrated that co-segregation of an amber suppressor with such alleles was required for viability of spores subsequent to meiosis. These mutations are expected to be useful in the identification of the molecular products of the genes cdc28 and cdc37.
UNEQUAL CROSSING OVER AT THE rRNA TANDON AS A SOURCE OF QUANTITATIVE GENETIC VARIATION IN DROSOPHILA
Authors: Frankham R , Briscoe D A , Nurthen R K
Published: April 2021

Abdominal bristle selection lines (three high and three low) and controls were founded from a marked homozygous line to measure the contribution of sex-linked "mutations" to selection response. Two of the low lines exhibited a period of rapid response to selection in females, but not in males. There were corresponding changes in female variance, in heritabilities in females, in the sex ratio (a deficiency of females) and in fitness, as well as the appearance of a mutant phenotype in females of one line. All of these changes were due to bb alleles (partial deficiencies for the rRNA tandon) in the X chromosomes of these lines, while the Y chromosomes remained wild-type bb+. We argue that the bb alleles arose by unequal crossing over in the rRNA tandon.—A prediction of this hypothesis is that further changes can occur in the rRNA tandon as selection is continued. This has now been shown to occur.—Our minimum estimate of the rate of occurrence of changes at the rRNA tandon is 3 × 10-4. As this is substantially higher than conventional mutation rates, the questions of the mechanisms and rates of origin of new quantitative genetic variation require careful re-examination.
TRANSLATIONAL COUPLING DURING EXPRESSION OF THE TRYPTOPHAN OPERON OF ESCHERICHIA COLI
Authors: Oppenheim Daniel S , Yanofsky Charles
Published: April 2021

E. coli trpE polar mutations are 10 times more polar on trpD gene expression than on downstream (trpC, B, or A) gene expression. This effect was shown to be the result of "translational coupling," in which efficient translation of trpD mRNA reqiures efficient translation of the end of trpE mRNA. The trpE-trpD intercistronic punctuation region consists of overlapping stop and start codons, and the trpE and trpD gene products form a functional complex in the cell. In light of these observations and characteristics, several models for the mechanism of translational coupling are considered.
INTRACELLULAR POPULATION GENETICS: EVIDENCE FOR RANDOM DRIFT OF MITOCHONDRIAL ALLELE FREQUENCIES IN SACCHAROMYCES CEREVISIAE AND SCHIZOSACCHAROMYCES POMBE
Authors: Thrailkill Kathryn M , Birky C William , Lückemann Gudrun , Wolf Klaus
Published: April 2021

We report evidence for random drift of mitochondrial allele frequencies in zygote clones of Saccharomyces cerevisiae and Schizosaccharomyces pombe. Monofactorial and bifactorial crosses were done, using strains resistant or sensitive to erythromycin (alleles ER, ES), oligomycin (OR, OS), or diuron (DR, DS). The frequencies of resistant and sensitive cells (and thus the frequencies of the resistant and sensitive alleles) were determined for each of a number of clones of diploid cells arising from individual zygotes. Allele frequencies were extremely variable among these zygote clones; some clones were "uniparental," with mitochondrial alleles from only one parent present. These observations suggest random drift of the allele frequencies in the population of mitochondrial genes within an individual zygote and its diploid progeny. Drift would cease when all the cells in a clone become homoplasmic, due to segregation of the mitochondrial genomes during vegetative cell divisions. To test this, we delayed cell division (and hence segregation) for varying times by starving zygotes in order to give drift more time to operate. As predicted, delaying cell division resulted in an increase in the variance of allele frequencies among the zygote clones and an increase in the proportion of uniparental zygote clones. The changes in form of the allele frequency distributions resembled those seen during random drift in finite Mendelian populations. In bifactorial crosses, genotypes as well as individual alleles were fixed or lost in some zygote clones. However, the mean recombination frequency for a large number of clones did not increase when cell division was delayed. Several possible molecular mechanisms for intracellular random drift are discussed.
INDUCTION AND SEGREGATION OF CHLOROPLAST MUTATIONS IN VEGETATIVE CELL CULTURES OF CHLAMYDOMONAS REINHARDTII
Authors: Lee Robert W , Haughn George W
Published: April 2021

The single chloroplast of the alga Chlamydomonas reinhardtii contains at least 100 copies of the chloroplast chromosome. It is not known how the chloroplast (or cell) becomes homoplasmic for a mutation that arises in one of these copies. Under suitable selection conditions, clones with chloroplast mutations for streptomycin resistance induced by methyl methanesulfonate can be recovered with direct plating after mutagenesis. Using an adaptation of the Luria-Delbrück fluctuation test, mutagenized cultures grown on nonselective liquid medium for seven to nine doublings show negligible proliferation of cells capable of forming such mutant colonies. In contrast, cells among the same cultures with reduced nuclear mutations conferring streptomycin resistance reveal considerable clonal propagation prior to plating on selection medium. Reconstruction growth-rate experiments show no reduced growth of cells with chloroplast mutations relative to either wild-type cells or to those with nuclear mutations. We propose that newly arising chloroplast mutations and their copies are usually transmitted to only one daughter cell for several cell generations by reductional divisions of the chloroplast genome. In the absence of recombination and mixing, such a reductional partition of chloroplast alleles would readily permit the formation of homoplasmic lines without the need for selection.
PROTOPLASMIC INCOMPATIBILITY IN PODOSPORA ANSERINA: A POSSIBLE FUNCTION FOR INCOMPATIBILITY GENES
Authors: Boucherie Hélian , Bernet Jean
Published: April 2021

The suppression of protoplasmic incompatibility resulting from nonallelic gene interactions has been obtained by the coupled effect of mutations in the modA and modB genes (Bernet 1971). Due to their female sterility, modA modB strains provide an experimental tool to determine whether or not the mod and incompatibility loci are involved in a function other than protoplasmic incompatibility. Present results show that modA modB female sterility is a nonautonomous trait since heterokaryotic mycelia that include a modA modB nucleus and a female fertile nucleus (wild-type, modA or modB) produce modA modB protoperithecia, which are also formed by culture on medium supplemented with specific amino acids. Using modA modB strains, which are sterile at 32° and fertile at 26°, we have shown that the mod genes have no specific sequential timing. Indeed, the mod mutations may prevent the achievement of the female sexual cycle at any developmental stage from before early differentiation of protoperithecia until ascospore maturation. Employing different modA and modB mutations, we have shown that protoperithecia in modA modB cultures are generally distributed in female fertile rings; this result indicates that protoperithecia occur only in mycelial areas that have a restricted range of age at the time that modA modB thalli complete growth. Furthermore, nonsense mutations of incompatibility genes suppress the modA modB female fertile rings or restrict their width, suggesting that incompatibility loci, like the mod loci, are involved in protoperithecium formation. Taken together, these results lead to the postulate that mod and incompatibility genes do not determine, sensu stricto, protoperithecial function, as previously supposed (Boucherie and Bernet 1974), but may be involved in the homeostatic control of stationary cell functions essential for the complete development of the female sexual cycle.
HIGH MUTABILITY IN MALE HYBRIDS OF DROSOPHILA MELANOGASTER
Authors: Simmons Michael J , Johnson Nancy A , Fahey Thomas M , Nellett Sue M , Raymond John D
Published: April 2021

The frequencies of sex-linked lethal mutations arising in hybrid male offspring from various crosses and in nonhybrid controls were determined. The hybrids were produced by crossing representative strains of the P-M system of hybrid dysgenesis in all possible combinations. Males from the cross of P males × M females had a mutation rate about 15 times higher than that of nonhybrid males from the P strain. Genetically identical males from the reciprocal cross had a mutation rate 3 to 4 times that of the nonhybrids. For crosses involving a Q strain, a significant increase in the mutation rate was detected in males produced by matings of Q males with M females. No increase was observed in genetically identical males from the reciprocal mating. Crosses between P and Q strains gave male hybrids with mutation rates not different from those of nonhybrids. Many of the lethals that occurred in hybrids from the cross of P males × M females appeared to be unstable; fewer lethals that arose in hybrids from the cross of Q males × M females were unstable. The relationship between P and Q strains is discussed with respect to a model of mutation induction in dysgenic hybrids.
LINKAGE DISEQUILIBRIUM IN ISOLATED POPULATIONS OF DROSOPHILA MELANOGASTER
Authors: Yamaguchi Osamu , Ichinose Motoshi , Matsuda Muneo , Mukai Terumi
Published: April 2021

In order to test the interaction effect of overdominance and random genetic drift on the formation of linkage disequilibria under the condition of multiplicative gene action, linkage disequilibria between isozyme genes, inter se, and between polymorphic inversions and isozyme genes were tested for the second chromosomes of Drosophila melanogaster sampled from two isolated Pacific islands and one locality of the northern district of the mainlands of Japan. The effective sizes of these populations were known to be approximately 3,000 to 6,000 on the basis of the allelism rate of lethal chromosomes and their frequencies. The following results were obtained: (1) No linkage disequilibrium considered to be induced by epistasis, including the interaction between overdominance and random genetic drift, was detected. (2) Nonrandom association between polymorphic inversions and isozyme genes that are included in the inversions or located in the adjacent region outside the inversions was detected. (3) On the basis of the comparison of χ2 values, indicating the magnitudes of linkage disequilibrium, between the present isolated populations and the Raleigh, N. C., population (Mukai et al. 1974, 1977), the characteristics of the isolated populations and the nature of these linkage disequilibria are discussed.
THE TRIANGLE INEQUALITY IN BALANCED GENETICAL POLYMORPHISMS
Authors: Mandel S P H
Published: April 2021

A simplified derivation of the "triangle inequality," first obtained by Lewontin, Ginzburg and Tuljapurkar (1978), is presented.
ASSOCIATION OF DISOMIC CHROMOSOME LOSS WITH EMS-INDUCED CONVERSION IN YEAST
Authors: Campbell Douglas
Published: April 2021

Experimental tests with the yeast Saccharomyces cerevisiae of a previously proposed model suggesting a causal relationship between disomic chromosome loss (n + 1 → n) and centromere-adjacent mitotic gene conversion were performed. Disomic haploid cells heteroallelic at two loci on the left arm of chromosome III were exposed to ethyl methanesulfonate (EMS) under nonlethal conditions; EMS-induced prototrophic gene convertants were selected and tested for coincident chromosome loss. The principal results are: (1) The frequency of chromosome loss among EMS-induced gene convertants selected to arise near the centromere is markedly enhanced over basal levels and remains constant, independent of EMS exposure. There is little such enhancement among EMS-induced convertants selected to arise far from the centromere. (2) Chromosome loss is almost completely associated with induced conversion of the centromere-proximal allele at the centromere-adjacent heteroallelic locus. This result is identical to (and confirms) results found previously for spontaneous loss-associated conversion. (3) The conversion polarity at the centromere-adjacent locus among unselected (nonloss-associated) induced or spontaneous mitotic convertants is identical to that among meiotic convertants and markedly favors the contromere-distal allele. These findings are wholly consistent with, and strengthen, the hypothesis that structural involvement of centromeric regions in nearby recombinational events may interfere with proper segregational function and lead to mitotic chromosome loss.
ULTRASTRUCTURAL ANALYSIS OF SPERMIOGENESIS IN SEGREGATION DISTORTER MALES OF DROSOPHILA MELANOGASTER: THE HOMOZYGOTES
Authors: Kettaneh Nizam P , Hartl Daniel L
Published: April 2021

We have studied spermiogenesis at the ultrastructural level in males of genotype SD(NH)-2/SD-72, which are nearly sterile owing to the dysfunction of virtually all of their sperm. Ultrastructural aspects of spermiogenesis in these homozygous SD males are qualitatively similar to those found among dysfunctional sperm produced by heterozygous SD males. In particular, chromatin condensation and/or compaction has been found to be abnormal. However, major quantitative differences have been noted. Most of the dysfunctional sperm in SD(NH)-2/SD-72 males are individualized and coiled. Then, the sperm evidently undergo degeneration, as few mature sperm can be found in the seminal vesicle. The relevance of these findings to the mechanism leading to near sterility in homozygous SD males is discussed.
GENETIC DISSECTION OF SEGREGATION DISTORTION. III. UNEQUAL RECOVERY OF RECIPROCAL RECOMBINANTS
Authors: Hartl Daniel L
Published: April 2021

The genetic structure of a segregation distorter chromosome (a derivative of SD-36) has been analyzed in a system in which recombination in the second chromosome is blocked by inversions except for the critical region around the centromeric heterochromatin. The results confirm the map order and characteristics of four loci known to be involved in segregation distortion, namely Sd, E(SD), Rspins, M(SD). However, SD-36 carries a fifth major locus involved in distortion. This locus is near pr in 2L and has the effect of enhancing the degree of distortion. In addition, reciprocal recombinant products from SD-36 are recovered unequally. All recombinants carrying the pr region from SD-36 seem also to carry Sd, although Sd has previously been mapped 1.6 units to the left of pr. Both the enhancement of distortion and the unequal recovery of reciprocal products can be explained if it is assumed that the new locus near pr in SD-36 is actually a duplication of Sd.
GENETIC CHANGES IN FLUCTUATING VOLE POPULATIONS: SELECTIVE VS. NONSELECTIVE FORCES
Authors: Gaines Michael S , Whittam Thomas S
Published: April 2021

The Lewontin-Krakauer test for selective neutrality of polymorphisms was applied to temporal variation in gene frequency at five electrophoretic loci in each of four fluctuating vole populations over a three-year period. The results indicate that changes in gene frequencies were primarily due to nonselective forces.
ON THE EVOLUTION OF NEW METABOLIC FUNCTIONS IN DIPLOID ORGANISMS
Authors: Hall Barry G
Published: April 2021

Evolution of lactose utilization via the ebg system of Escherichia coli requires both structural gene (ebgA) and regulatory gene (ebgR) mutations. Because evolution of new metabolic functions in diploids might be subject to constraints not present in haploid organisms, merodiploid strains carrying a wild-type and an evolved ebgA allele, or a wild-type and an evolved ebgR allele were constructed. I show that heterozygosity at ebgA does not significantly affect the selective advantage of the evolved ebgA allele; whereas heterozygosity at ebgR eliminates the selective advantage of the evolved ebgR allele. It is suggested that, in diploid organisms, evolution of new functions for systems under negative control would be very difficult.
THE ROLE OF DOSAGE OF THE REGION 7D1–7D5-6 OF THE X CHROMOSOME IN THE PRODUCTION OF HOMEOTIC TRANSFORMATIONS IN DROSOPHILA MELANOGASTER
Authors: Gans M , Forquignon F , Masson M
Published: April 2021

A high frequency of homeotic transformations appears in Df(3)red/+ progeny of Df(1)snC128 /+ females. Generally, the metathoracic appendages are partially transformed into mesothoracic ones. Df(1)snC128 includes a small region of the X chromosome: 7D1 to 7D5-6. Hypodosage of this region is mainly effective at the level of the maternal genotype, and the effect is probably due to hypodosage of the wild-type allele of the gene fs(1)h. Df(3)red has an effect that is mainly, if not exclusively, zygotic, probably due to hypodosage of the wild-type allele of Rg-bx. The frequencies of transformed flies resulting from the interaction between Df(1)snC128 and Df(3)red are not very sensitive to external conditions and genetic background. Studies of the interactions between Df(1)snC128 and other mutations or deficiencies of chromosome 3 [Rg-pbx, bx, pbx, Ubx1, Ubx130, Ubx80, Df(3)P9] reveal an analogy between the hypodosage effect of region 7D1–7D5-6 and the effects of ether treatment of blastoderm stage eggs. The role of the gene fs(1)h in the process of segment determination is discussed in the light of these results.
VARIATION IN BIOCHEMICAL PROPERTIES OF ALLOZYMES OF XANTHINE DEHYDROGENASE IN DROSOPHILA PSEUDOOBSCURA
Authors: Wilcox David R , Prakash Satya
Published: April 2021

Twenty-six D. pseudoobscura strains isogenic for xanthine dehydrogenase alleles from Mesa Verde, Colorado, were tested for differences in the biochemical properties of different allelic forms of xanthine dehydrogenase. No significant differences in binding affinity (Km) or substrate specificity of the enzyme were found. Significant variation among strains, in activity (V  max) and among electromorphs, as well as among strains, in thermolability was found. For the few strains tested, the activity and thermolability differences were shown to co-segregate with the electrophoretic mobility of the variant allele.
CRYPTIC OPERON FOR β-GLUCOSIDE METABOLISM IN ESCHERICHIA COLI K12: GENETIC EVIDENCE FOR A REGULATORY PROTEIN
Authors: Defez Roberto , De Felice Maurilio
Published: April 2021

Escherichia coli K12 does not metabolize β-glucosides such as arbutin and salicin because of lack of expression of the bglBSRC operon, which contains structural genes for transport (bglC) and hydrolysis (bglB) of phospho-β-glucosides. Mutants carrying lesions in the cis-acting regulatory site bglR metabolize β-glucosides as a consequence of expression of this cryptic operon (Prasad and Schaefler 1974). We isolated mutations promoting β-glucoside metabolism that were unlinked to bglR; some of these mutations were shown to be amber. All of them were mapped at 27 min on the E. coli K12 linkage map and appeared to define a single gene, for which we propose the designation bglY. Utilization of β-glucosides in bglY mutants appeared to be a consequence of expression of the bglBSRC operon, since bglB bglR and bglB bglY double mutants had the same phenotype. All bglY mutations analyzed were recessive to the wild-type bglY  + allele. Phospho-β-glucosidase B and β-glucoside transport activities are inducible in bglY mutants, as they are in bglR mutants. Metabolism of β-glucosides in both bglR and bglY mutants required cyclic AMP. We propose that bglY encodes a protein acting as a repressor of the bglBSRC operon, active in both the presence and absence of β-glucosides, whose recognition site would be within the bglR locus.