bluuebunny/crossref_metadata_2025 huggingface dataset:
Channel Development and Electric Parameter Characteristics of Regular Pulse Bursts in Lightning
Authors: Fan X. , Zhang Y. , Krehbiel P. , Zheng D. , Yao W. , Zhang Y. , Xu L. , Liu H. , Lyu W.
Published: April 2024

Observations of a regular pulse burst (RPB) at the end of a K‐event are analyzed utilizing a simple geometric model and particle swarm optimization (PSO) to estimate the currents and propagation speeds of successive pulses of the RPB. The results show that the current of successive pulses is strongly overlapped and, for typical speeds of continuously propagating K‐events, are unphysically large (88 kA), exceeding the currents of most strokes to ground. By default, the unphysical nature of the result, coupled with very high frequency interferometer observations of an RPB in Florida, shows that the propagation speed of the pulses is significantly faster than expected, namely ∼0.6–1.8 × 108 m/s. This reduces the inferred current from 88 kA down to 6–18 kA, typical of intracloud events. The fast propagation speed of the stepping is explained by successive pulses retracing much of the path of the preceding pulses due to the successive pulses being strongly overlapped.
Statistical Analysis of Off‐Great Circle Radio Wave Propagation in the Polar Cap
Authors: Cameron T. G. , Fiori R. A. D. , Perry G. W. , Spicher A. , Thayaparan T.
Published: April 2024

High latitude ionospheric density structures such as polar cap patches and arcs are capable of deflecting high frequency (HF) radio waves to off‐great circle paths, and are likely detrimental to technologies dependent on HF radio propagation. In this study, nearly 2.5 years of 4.6–14.4 MHz data from a multi‐frequency HF radio link between Qaanaaq, Greenland and Alert, Canada are used to investigate high‐latitude off‐great circle propagation in the polar cap. After an example of HF radio propagation affected by polar cap patches is shown in detail, a statistical analysis of the occurrence and impacts of off‐great circle deflections in the polar cap is presented. Off‐great circle propagation is shown to be increasingly common with increasing frequency up to 11.1 MHz, such that averaged over 1 year, received 11.1 MHz signals experienced deflections >30° from the great circle direction 65.6% of the time. The occurrence of these deflections across the year is shown to be at a maximum in the winter, while occurrence across the day varies with season. Trends across both time of day and time of year for 11.1 and 14.4 MHz deflections are consistent with polar cap patch occurrence trends. Off‐great circle deflections are shown to be associated with increased time‐of‐flights, a larger range of positive and negative Doppler shifts, increased Doppler spreads, and lower signal‐to‐noise ratios. These results are discussed in the context of ionospheric phenomena in the polar cap, and implications for over‐the‐horizon radars operating at high latitudes.
Effect of Seismicity and Tectonic‐Glacial Interactions on Submarine Megaslides
Authors: Gulick Sean P. S. , Reece Robert S. , Sawyer Derek E. , Christeson Gail L. , Horton Brian K.
Published: April 2024

Enhanced sedimentation at glacial margins can produce submarine megaslides (>10,000 km3). We report a single megaslide in the Surveyor Fan, Gulf of Alaska. Minimum extant size is ∼16,124 km2 in area and ∼9,080 km3 in volume. Slope failure occurred ∼1.2 Ma at the onset of the mid‐Pleistocene transition (MPT). With accretion along the Aleutian‐Alaska Trench, the original volume is conservatively ∼16,280 km3, with only a 140 km run‐out due to its blocky, high shear strength nature. We suggest the megaslide was triggered by a major sediment influx at the onset of the MPT, when glacial‐interglacial cycles shifted from 41 to 100 Kyr. The absence of repeat megaslides may reflect a changing balance between seismic strengthening and sediment flux, where later sedimentation driven by cross‐shelf ice streams results in thin, fluidized, non‐cohesive slides. Continued accretion of the Surveyor Fan and megaslide also reduces critical wedge taper, further inhibiting major failure.
Thank You to Our 2023 Reviewers
Authors: Salous Sana , Kobayashi Kazuya
Published: April 2024

Key Points

The editors thank the 2023 peer reviewers
Usual intake of dairy products and the chance of pre-diabetes regression to normal glycemia or progression to type 2 diabetes: a 9-year follow-up
Authors: Bahadoran Zahra , Mirmiran Parvin , Azizi Fereidoun
Published: April 2024

Background

We assessed the possible effect of usual dairy consumption on pre-diabetes (Pre-DM) remission or progression to type 2 diabetes (T2D).

Methods

Pre-DM adults (n = 334, mean age of 49.4 years, and 51.5% men) were assessed for dairy intakes (2006–2008) and followed up to 9 years for incidence of T2D or normal glycemia (NG). All biochemical measurements were done at baseline and all subsequent examinations with 3-y follow-up intervals. Multinomial regression models with adjustment of confounding variables were used to estimate odds ratios (OR) and 95% confidence intervals (CIs) of incident T2D and NG for each serving/d dairy consumption.

Results

The odds of NG was significantly elevated by 69% (OR = 1.69, 95% CI = 1.00–2.86, P = 0.05) per 200 g/d increased high-fat dairy intake, while the amount of total dairy or low-fat dairy was not related to the outcomes. Higher intakes of yogurt were more likely to be associated with an increased odds of NG (OR = 1.82, 95% CI = 1.20–2.74, P = 0.01). Usual intakes of milk, cheese, or cream-butter were not associated to Pre-DM remission or progression to T2D.

Conclusion

Regular dairy consumption may increase the chance of Pre-DM regression to NG.
Association of food insecurity with changes in diet quality, weight, and glycemia over two years in adults with prediabetes and type 2 diabetes on medicaid
Authors: Gu Kristine D. , Cheng Jessica , Fung Vicki , Levy Douglas E. , McGovern Sydney , McCurley Jessica L. , Clark Cheryl R. , Thorndike Anne N.
Published: April 2024

Little is known about longitudinal associations between food insecurity (FI) and diet, weight, and glycemia in people with prediabetes and type 2 diabetes (T2D). In a secondary analysis of Medicaid-enrolled health center patients with prediabetes or T2D in Boston, Massachusetts (N = 188), we examined associations between food security (FS) and measures of diet quality, weight, and hyperglycemia. FS (10-item USDA FS module) was ascertained at baseline, 1-year, and 2-year follow-up and categorized as persistently secure, intermittently insecure, or persistently insecure. Associations between FS category and changes in Healthy Eating Index-2020 (HEI-20), body mass index (BMI), and hemoglobin A1c (A1c) from baseline to year 2 were assessed using multivariate generalized linear models. Participants had median (p25, p75) age of 52 (42, 57); 71.8% were female and 62.8% Hispanic. Over follow-up, 32.4% were persistently food secure, 33.0% intermittently insecure, and 34.5% persistently insecure. Baseline mean (SD) HEI-20, BMI, and A1c were 55.8 (14.5), 35.9 (8.7) kg/m2, 7.1% (1.6) and did not differ by FS category. FS category was not associated with changes in HEI-20, BMI, and A1c at 2 years (all p > 0.05). Results suggest that Medicaid-enrolled adults with prediabetes or T2D, regardless of FS status, would benefit from dietary and weight management interventions.
Dynamic genomic changes in methotrexate-resistant human cancer cell lines beyond DHFR amplification suggest potential new targets for preventing drug resistance
Authors: Meng Xiang-Ning , Ma Jin-Fa , Liu Yang-He , Li Si-Qing , Wang Xu , Zhu Jing , Cai Meng-Di , Zhang Hui-Shu , Song Tiantian , Xing Shukai , Hou Li-Qing , Guo Huan , Cui Xiao-Bo , Han Jiang , Liu Peng , Ji Guo-Hua , Sun Wen-Jing , Yu Jing-Cui , Fu Song-Bin
Published: April 2024

Background

Although DHFR gene amplification has long been known as a major mechanism for methotrexate (MTX) resistance in cancer, the early changes and detailed development of the resistance are not yet fully understood.

Methods

We performed genomic, transcriptional and proteomic analyses of human colon cancer cells with sequentially increasing levels of MTX-resistance.

Results

The genomic amplification evolved in three phases (pre-amplification, homogenously staining region (HSR) and extrachromosomal DNA (ecDNA)). We confirm that genomic amplification and increased expression of DHFR, with formation of HSRs and especially ecDNAs, is the major driver of resistance. However, DHFR did not play a detectable role in the early phase. In the late phase (ecDNA), increase in FAM151B protein level may also have an important role by decreasing sensitivity to MTX. In addition, although MSH3 and ZFYVE16 may be subject to different posttranscriptional regulations and therefore protein expressions are decreased in ecDNA stages compared to HSR stages, they still play important roles in MTX resistance.

Conclusion

The study provides a detailed evolutionary trajectory of MTX-resistance and identifies new targets, especially ecDNAs, which could help to prevent drug resistance. It also presents a proof-of-principal approach which could be applied to other cancer drug resistance studies.
Dietary factors and their influence on immunotherapy strategies in oncology: a comprehensive review
Authors: Golonko Aleksandra , Pienkowski Tomasz , Swislocka Renata , Orzechowska Sylwia , Marszalek Krystian , Szczerbinski Lukasz , Swiergiel Artur Hugo , Lewandowski Wlodzimierz
Published: April 2024

Immunotherapy is emerging as a promising avenue in oncology, gaining increasing importance and offering substantial advantages when compared to chemotherapy or radiotherapy. However, in the context of immunotherapy, there is the potential for the immune system to either support or hinder the administered treatment. This review encompasses recent and pivotal studies that assess the influence of dietary elements, including vitamins, fatty acids, nutrients, small dietary molecules, dietary patterns, and caloric restriction, on the ability to modulate immune responses. Furthermore, the article underscores how these dietary factors have the potential to modify and enhance the effectiveness of anticancer immunotherapy. It emphasizes the necessity for additional research to comprehend the underlying mechanisms for optimizing the efficacy of anticancer therapy and defining dietary strategies that may reduce cancer-related morbidity and mortality. Persistent investigation in this field holds significant promise for improving cancer treatment outcomes and maximizing the benefits of immunotherapy.
Decoding spatiotemporal transcriptional dynamics and epithelial fibroblast crosstalk during gastroesophageal junction development through single cell analysis
Authors: Kumar Naveen , Prakash Pon Ganish , Wentland Christian , Kurian Shilpa Mary , Jethva Gaurav , Brinkmann Volker , Mollenkopf Hans-Joachim , Krammer Tobias , Toussaint Christophe , Saliba Antoine-Emmanuel , Biebl Matthias , Jürgensen Christian , Wiedenmann Bertram , Meyer Thomas F. , Gurumurthy Rajendra Kumar , Chumduri Cindrilla
Published: April 2024

The gastroesophageal squamocolumnar junction (GE-SCJ) is a critical tissue interface between the esophagus and stomach, with significant relevance in the pathophysiology of gastrointestinal diseases. Despite this, the molecular mechanisms underlying GE-SCJ development remain unclear. Using single-cell transcriptomics, organoids, and spatial analysis, we examine the cellular heterogeneity and spatiotemporal dynamics of GE-SCJ development from embryonic to adult mice. We identify distinct transcriptional states and signaling pathways in the epithelial and mesenchymal compartments of the esophagus and stomach during development. Fibroblast-epithelial interactions are mediated by various signaling pathways, including WNT, BMP, TGF-β, FGF, EGF, and PDGF. Our results suggest that fibroblasts predominantly send FGF and TGF-β signals to the epithelia, while epithelial cells mainly send PDGF and EGF signals to fibroblasts. We observe differences in the ligands and receptors involved in cell-cell communication between the esophagus and stomach. Our findings provide insights into the molecular mechanisms underlying GE-SCJ development and fibroblast-epithelial crosstalk involved, paving the way to elucidate mechanisms during adaptive metaplasia development and carcinogenesis.
LDL receptor-related protein 5 selectively transports unesterified polyunsaturated fatty acids to intracellular compartments
Authors: Tang Wenwen , Luan Yi , Yuan Qianying , Li Ao , Chen Song , Menacherry Stanley , Young Lawrence , Wu Dianqing
Published: April 2024

Polyunsaturated fatty acids (PUFAs), which cannot be synthesized by animals and must be supplied from the diet, have been strongly associated with human health. However, the mechanisms for their accretion remain poorly understood. Here, we show that LDL receptor-related protein 5 (LRP5), but not its homolog LRP6, selectively transports unesterified PUFAs into a number of cell types. The LDLa ligand-binding repeats of LRP5 directly bind to PUFAs and are required and sufficient for PUFA transport. In contrast to the known PUFA transporters Mfsd2a, CD36 and FATP2, LRP5 transports unesterified PUFAs via internalization to intracellular compartments including lysosomes, and n-3 PUFAs depend on this transport mechanism to inhibit mTORC1. This LRP5-mediated PUFA transport mechanism suppresses extracellular trap formation in neutrophils and protects mice from myocardial injury during ischemia-reperfusion. Thus, this study reveals a biologically important mechanism for unesterified PUFA transport to intracellular compartments.
Tetris-inspired detector with neural network for radiation mapping
Authors: Okabe Ryotaro , Xue Shangjie , Vavrek Jayson R. , Yu Jiankai , Pavlovsky Ryan , Negut Victor , Quiter Brian J. , Cates Joshua W. , Liu Tongtong , Forget Benoit , Jegelka Stefanie , Kohse Gordon , Hu Lin-wen , Li Mingda
Published: April 2024

Radiation mapping has attracted widespread research attention and increased public concerns on environmental monitoring. Regarding materials and their configurations, radiation detectors have been developed to identify the position and strength of the radioactive sources. However, due to the complex mechanisms of radiation-matter interaction and data limitation, high-performance and low-cost radiation mapping is still challenging. Here, we present a radiation mapping framework using Tetris-inspired detector pixels. Applying inter-pixel padding for enhancing contrast between pixels and neural networks trained with Monte Carlo (MC) simulation data, a detector with as few as four pixels can achieve high-resolution directional prediction. A moving detector with Maximum a Posteriori (MAP) further achieved radiation position localization. Field testing with a simple detector has verified the capability of the MAP method for source localization. Our framework offers an avenue for high-quality radiation mapping with simple detector configurations and is anticipated to be deployed for real-world radiation detection.
Carbon dioxide capture and functionalization by bis(N-heterocyclic carbene)-borylene complexes
Authors: Fan Jun , Koh An-Ping , Wu Chi-Shiun , Su Ming-Der , So Cheuk-Wai
Published: April 2024

Derivatives of free monocoordinated borylenes have attracted considerable interest due to their ability to exhibit transition-metal-like reactivity, in particular small molecules capture. However, such complexes are rare as the formation is either endergonic, or the resulting adduct is a transient intermediate that is prone to reaction. Here, we present the synthesis of two bis(N-heterocyclic carbene)-borylene complexes capable of capturing and functionalizing carbon dioxide. The capture and subsequent functionalization of CO2 by the bis(NHC)-disilylamidoborylene 1 is demonstrated by the formation of the bis(NHC)-isocyanatoborylene-carbon dioxide complex 3. Reversible capture of CO2 is observed using the bis(NHC)-mesitylborylene 2, and the persistent bis(NHC)-mesitylborylene-carbon dioxide adduct 4 can be stabilized by hydrogen bonding with boric acid. The reactions of 4 with ammonia-borane and aniline demonstrate that the captured CO2 can be further functionalized.
Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin
Authors: Tyson Jess , Radford Paul , Lambert Carey , Till Rob , Huwiler Simona G. , Lovering Andrew L. , Elizabeth Sockett R.
Published: April 2024

The bacterium Bdellovibrio bacteriovorus is a predator of other Gram-negative bacteria. The predator invades the prey’s periplasm and modifies the prey’s cell wall, forming a rounded killed prey, or bdelloplast, containing a live B. bacteriovorus. Redundancy in adhesive processes makes invasive mutants rare. Here, we identify a MIDAS adhesin family protein, Bd0875, that is expressed at the predator-prey invasive junction and is important for successful invasion of prey. A mutant strain lacking bd0875 is still able to form round, dead bdelloplasts; however, 10% of the bdelloplasts do not contain B. bacteriovorus, indicative of an invasion defect. Bd0875 activity requires the conserved MIDAS motif, which is linked to catch-and-release activity of MIDAS proteins in other organisms. A proteomic analysis shows that the uninvaded bdelloplasts contain B. bacteriovorus proteins, which are likely secreted into the prey by the Δbd0875 predator during an abortive invasion period. Thus, secretion of proteins into the prey seems to be sufficient for prey killing, even in the absence of a live predator inside the prey periplasm.
Transplantation of gut microbiota derived from patients with schizophrenia induces schizophrenia-like behaviors and dysregulated brain transcript response in mice
Authors: Wei Nana , Ju Mingliang , Su Xichen , Zhang Yan , Huang Yonghe , Rao Xinyue , Cui Li , Lin Zhibing , Dong Yi
Published: April 2024

Schizophrenia (SCZ), as a neurodevelopmental disorder and devastating disease, affects approximately 1% of the world population. Although numerous studies have attempted to elucidate the causes of SCZ occurrence, it is not clearly understood. Recently, the emerging roles of the gut microbiota in a range of brain disorders, including SCZ, have attracted much attention. While the molecular mechanism of gut microbiota in regulating the pathogenesis of SCZ is still lacking. Here, we first confirmed the difference of gut microbiome between SCZ patients and healthy controls, and then, we performed fecal microbiota transplantation (FMT) to clarify the roles of SCZ patients-derived microbiota in a specific pathogen free (SPF) mice model. 16 S rDNA sequencing confirmed that a significant difference of gut microbiome was present between two groups of FMT mice, which has a similar trend with the above human gut microbiome. Furthermore, we found that transplantation of fecal microbiota from SCZ patients into SPF mice was sufficient to induce schizophrenia-like (SCZ-like) symptoms, such as deficits in sociability and hyperactivity. Furthermore, the brains of mice colonized with SCZ microbiota displayed dysregulated transcript response and alternative splicing of SCZ-relevant genes. Moreover, 10 key genes were identified to be correlated with SCZ by an integrative transcriptome data analysis. Finally, 4 key genes were identified to be correlated with the 12 differential genera between two groups of FMT mice. Our results thus demonstrated that the gut microbiome might modify the transcriptomic profile in the brain, thereby modulating social behavior, and our present study can help better understand the link between gut microbiota and SCZ pathogenesis through the gut-brain axis.
Three million images and morphological profiles of cells treated with matched chemical and genetic perturbations
Authors: Chandrasekaran Srinivas Niranj , Cimini Beth A. , Goodale Amy , Miller Lisa , Kost-Alimova Maria , Jamali Nasim , Doench John G. , Fritchman Briana , Skepner Adam , Melanson Michelle , Kalinin Alexandr A. , Arevalo John , Haghighi Marzieh , Caicedo Juan C. , Kuhn Daniel , Hernandez Desiree , Berstler James , Shafqat-Abbasi Hamdah , Root David E. , Swalley Susanne E. , Garg Sakshi , Singh Shantanu , Carpenter Anne E.
Published: April 2024

The identification of genetic and chemical perturbations with similar impacts on cell morphology can elucidate compounds’ mechanisms of action or novel regulators of genetic pathways. Research on methods for identifying such similarities has lagged due to a lack of carefully designed and well-annotated image sets of cells treated with chemical and genetic perturbations. Here we create such a Resource dataset, CPJUMP1, in which each perturbed gene’s product is a known target of at least two chemical compounds in the dataset. We systematically explore the directionality of correlations among perturbations that target the same protein encoded by a given gene, and we find that identifying matches between chemical and genetic perturbations is a challenging task. Our dataset and baseline analyses provide a benchmark for evaluating methods that measure perturbation similarities and impact, and more generally, learn effective representations of cellular state from microscopy images. Such advancements would accelerate the applications of image-based profiling of cellular states, such as uncovering drug mode of action or probing functional genomics.
Ginkgolic acid inhibits orthopneumo- and metapneumo- virus infectivity
Authors: Luck Maria I. , Subillaga Erick J. , Borenstein Ronen , Sabo Yosef
Published: April 2024

The human respiratory syncytial virus (hRSV) and the human metapneumovirus (hMPV) are important human respiratory pathogens from the Pneumoviridae family. Both are responsible for severe respiratory tract infections in infants, young children, elderly individuals, adults with chronic medical conditions, and immunocompromised patients. Despite their large impact on human health, vaccines for hRSV were only recently introduced, and only limited treatment options exist. Here we show that Ginkgolic acid (GA), a natural compound from the extract of Ginkgo biloba, with known antiviral properties for several viruses, efficiently inhibits these viruses’ infectivity and spread in cultures in a dose-dependent manner. We demonstrate that the drug specifically affects the entry step during the early stages on the viruses’ life cycle with no effect on post-entry and late stage events, including viral gene transcription, genome replication, assembly and particles release. We provide evidence that GA acts as an efficient antiviral for members of the Pneumoviridae family and has the potential to be used to treat acute infections.
A tripe diffusion bioconvective model for thixotropic nanofluid with applications of induced magnetic field
Authors: Albedah Mohammed A. , Li Zhixiong , Tlili Iskander
Published: April 2024

Owing to enhanced thermal characteristics of nanomaterials, multidisciplinary applications of such particles have been utilized in the industrial and engineering processes, chemical systems, solar energy, extrusion processes, nuclear systems etc. The aim of current work is to suggests the thermal performances of thixotropic nanofluid with interaction of magnetic force. The suspension of microorganisms in thixotropic nanofluid is assumed. The investigation is further supported with the triple diffusion flow. The motivations for considering the triple diffusion phenomenon are associated to attaining more thermal applications. The flow pattern is subject to novel stagnation point flow. The convective thermal constraints are incorporated. The modeled problem is numerically evaluated by using shooting technique. Different consequences of physical parameters involving the problem are graphically attributed. The insight analysis is presented for proposed problem with different engineering applications. It is claimed that induced magnetic field enhanced due to magnetic parameter while declining results are observed for thixotropic parameter. The heat transfer enhances due to variation of Dufour number. Furthermore, low profile of nanoparticles concentration has been observed for thixotropic parameter and nano-Lewis number.
Polysacharide of Agaricus blazei gel mitigates bone necrosis in model of the jaws related to bisphosphonate via Wnt signaling
Authors: Costa de Sousa Vanessa , Nunes Sousa Fátima Regina , Felipe Vasconcelos Raquel , Angelino Barreto Gisele , Martins Conceição S. , Romero Dias Nilson , Costa Sislana , Chaves Bernardino Maria Jennifer , de Almeida Silva George , Linhares Nadine , Gondim Delane , Marques Mirna , Chaves Helliada , Alves Karuza , Leitão Renata , Brito Gerly A. C. , Nobre Pinho Ribeiro Maria Elenir , Goes Paula
Published: April 2024

To investigate de effect of PAb gel on the bone tissue of rats submitted to Bisphosphonate-related osteonecrosis of the jaws (BRONJ). Initially, 54 animals were submitted to BRONJ model by Zoledronic Acid (ZA) (0.1 mg/kg 3x/wk for 9 wk, ip), followed by the 1st upper left molar extraction at the 8th wk. After tooth removal, the animals were divided into 3 groups, ZA that received placebo gel or PAb gel that received 1% PAb gel, inside the dental alveolus. The control Group (CONTROL) received 0.1 mg/kg of 0.9% saline and then placebo gel. Three weeks after tooth extraction, the animals were euthanized, and maxillae were colleted for macroscopic, radiographic, histological and Raman spectomery assays. Additionally, GSK3b, beta-catenin, and Runx2 mRNA expressions were determined. Blood samples were collected for the analysis of Bone-specific alkaline phosphatase (BALP) levels. PAb gel improved mucosal healing, increased the number of viable osteocytes, while it reduced the number of empty lacunae, as well as the amount of bone sequestration. Furthermore, PAb gel positively influenced the number and functionality of osteoblasts by stimulating Wnt signaling, thereby inducing bone remodeling. Additionally, PAb gel contributed to improved bone quality, as evidenced by an increase in bone mineral content, a decrease in bone solubility, and an enhancement in the quality of collagen, particularly type I collagen. PAb gel mitigated bone necrosis by stimulating of bone remodeling through Wnt signaling and concurrently improved bone quality. PAb gel emerges as a promising pharmacological tool for aiding in BRONJ therapy or potentially preventing the development of BRONJ.
A secure and highly efficient blockchain PBFT consensus algorithm for microgrid power trading
Authors: Yao Zhongyuan , Fang Yonghao , Pan Heng , Wang Xiangyang , Si Xueming
Published: April 2024

There are a series of challenges in microgrid transactions, and blockchain technology holds the promise of addressing these challenges. However, with the increasing number of users in microgrid transactions, existing blockchain systems may struggle to meet the growing demands for transactions. Therefore, this paper proposes an efficient and secure blockchain consensus algorithm designed to meet the demands of large-scale microgrid electricity transactions. The algorithm begins by utilizing a Spectral clustering algorithm to partition the blockchain network into different lower-level consensus set based on the transaction characteristics of nodes. Subsequently, a dual-layer consensus process is employed to enhance the efficiency of consensus. Additionally, we have designed a secure consensus set leader election strategy to promptly identify leaders with excellent performance. Finally, we have introduced an authentication method that combines zero-knowledge proofs and key sharing to further mitigate the risk of malicious nodes participating in the consensus. Theoretical analysis indicates that our proposed consensus algorithm, incorporating multiple layers of security measures, effectively withstands blockchain attacks such as denial of service. Simulation experiment results demonstrate that our algorithm outperforms similar blockchain algorithms significantly in terms of communication overhead, consensus latency, and throughput.
The role of stroke-induced immunosuppression as a predictor of functional outcome in the neurorehabilitation setting
Authors: Vaghi Gloria , Morotti Andrea , Piella Elisa Maria , Avenali Micol , Martinelli Daniele , Cristina Silvano , Allena Marta , Grillo Valentina , Corrado Michele , Bighiani Federico , Cammarota Francescantonio , Antoniazzi Alessandro , Ferrari Federica , Mazzacane Federico , Cavallini Anna , Pichiecchio Anna , Rognone Elisa , Martinis Luca , Correale Luca , Castiglia Stefano Filippo , Trabassi Dante , Serrao Mariano , Tassorelli Cristina , De Icco Roberto
Published: April 2024

Stroke affects the interconnection between the nervous and immune systems, leading to a down-regulation of immunity called stroke-induced immunosuppression (SII). The primary aim of this study is to investigate SII role as a predictor of functional, neurological, and motor outcomes in the neurorehabilitation setting (NRB). We conducted a prospective observational study enrolling post-acute stroke patients hospitalized for neurorehabilitation. At NRB admission (T0) and discharge (T1), we assessed presence of SII (defined by a neutrophil-to-lymphocyte ratio ≥ 5) and we evaluated functional independence (Functional Independence Measure-FIM, Barthel Index-BI), motor performances (Tinetti Score, Hauser Ambulation Index) and neurological impairment (NIHSS). We enrolled 96 patients (45.8% females, 70.6 ± 13.9 years, 88.5% ischemic stroke). At T0, 15.6% of patients (15/96) had SII. When compared to immunocompetent patients (IC), the SII group was characterized by worse baseline functional independence, motor performances and neurological disability. The same was confirmed at T1 (FIM p = 0.012, BI p = 0.007, Tinetti p = 0.034, NIHSS p = 0.001). Neurological disability demonstrated a less pronounced improvement in SII (ΔNIHSS: SII: − 2.1 ± 2.3 vs. IC: − 3.1 ± 2.5, p = 0.035). SII group presented a higher percentage of infectious complications during the neurorehabilitation period (SII 80% vs. IC 25.9%; p = 0.001). SII may represent a negative prognostic factor in the neurorehabilitation setting. SII patients were characterized by poorer functional, motor, neurological performances and higher risk of infectious complications. ClinicaTrial registration: NCT05889169.
Utility of accessible SARS-CoV-2 specific immunoassays in vaccinated adults with a history of advanced HIV infection
Authors: Ferrari Ludovica , Ruggiero Alessandra , Stefani Chiara , Benedetti Livia , Piermatteo Lorenzo , Andreassi Eleonora , Caldara Federica , Zace Drieda , Pagliari Matteo , Ceccherini-Silberstein Francesca , Jones Christopher , Iannetta Marco , Geretti Anna Maria ,   The EVAN-COV Study Group, Ansaldo Lorenzo , Bertoli Ada , Bonfante Francesco , Braccialarghe Neva , Checchi Davide , Compagno Mirko , De Simone Giuseppe , Geretti Anna Maria , Grelli Sandro , Meloni Diletta , Mulas Tiziana , Piermatteo Lorenzo , Sarmati Loredana , Teti Elisabetta
Published: April 2024

Accessible SARS-CoV-2-specific immunoassays may inform clinical management in people with HIV, particularly in case of persisting immunodysfunction. We prospectively studied their application in vaccine recipients with HIV, purposely including participants with a history of advanced HIV infection. Participants received one (n = 250), two (n = 249) or three (n = 42) doses of the BNT162b2 vaccine. Adverse events were documented through questionnaires. Sample collection occurred pre-vaccination and a median of 4 weeks post-second dose and 14 weeks post-third dose. Anti-spike and anti-nucleocapsid antibodies were measured with the Roche Elecsys chemiluminescence immunoassays. Neutralising activity was evaluated using the GenScript cPass surrogate virus neutralisation test, following validation against a Plaque Reduction Neutralization Test. T-cell reactivity was assessed with the Roche SARS-CoV-2 IFNγ release assay. Primary vaccination (2 doses) was well tolerated and elicited measurable anti-spike antibodies in 202/206 (98.0%) participants. Anti-spike titres varied widely, influenced by previous SARS-CoV-2 exposure, ethnicity, intravenous drug use, CD4 counts and HIV viremia as independent predictors. A third vaccine dose significantly boosted anti-spike and neutralising responses, reducing variability. Anti-spike titres > 15 U/mL correlated with neutralising activity in 136/144 paired samples (94.4%). Three participants with detectable anti-S antibodies did not develop cPass neutralising responses post-third dose, yet displayed SARS-CoV-2 specific IFNγ responses. SARS-CoV-2 vaccination is well-tolerated and immunogenic in adults with HIV, with responses improving post-third dose. Anti-spike antibodies serve as a reliable indicator of neutralising activity. Discordances between anti-spike and neutralising responses were accompanied by detectable IFN-γ responses, underlining the complexity of the immune response in this population.
The oral cavity and intestinal microbiome in children with functional constipation
Authors: Kwiatkowska Monika , Gołębiewski Marcin , Sikora Marcin , Rycharska Ewa Łoś , Krogulska Aneta
Published: April 2024

Constipation is a widespread problem in paediatric practice, affecting almost 30% of children. One of the key causal factors of constipation may be disturbances in the homeostasis of the gastrointestinal microbiome. The aim of the study was to determine whether the oral and fecal microbiomes differ between children with and without constipation. A total of 91 children over three years of age were included in the study. Of these, 57 were qualified to a group with constipation, and 34 to a group without. The saliva and stool microbiomes were evaluated using 16S rRNA gene amplicon sequencing. Functional constipation was associated with characteristic bacterial taxa in the fecal microbiota. Statistically significant differences were found at the family level: Burkholderiaceae (q = 0.047), Christensenellaceae (q = 0.047), Chlostridiaceae (q = 0.047) were significantly less abundant in the constipation group, while the Tannerellaceae (q = 0.007) were more abundant. At the genus level, the significant differences were observed for rare genera, including Christensenellaceae r-7 (q = 2.88 × 10−2), Fusicatenibacter (q = 2.88 × 10−2), Parabacteroides (q = 1.63 × 10−2), Romboutsia (q = 3.19 × 10−2) and Subdoligranulum (q = 1.17 × 10−2). All of them were less abundant in children with constipation. With the exception of significant taxonomic changes affecting only feces, no differences were found in the alpha and beta diversity of feces and saliva. Children with functional constipation demonstrated significant differences in the abundance of specific bacteria in the stool microbiome compared to healthy children. It is possible that the rare genera identified in our study which were less abundant in the constipated patients (Christensellaceae r-7, Fusicatenibacter, Parabacteroides, Romboutsia and Subdoligranulum) may play a role in protection against constipation. No significant differences were observed between the two groups with regard to the saliva microbiome.
Impaired recognition of interactive intentions in adults with autism spectrum disorder not attributable to differences in visual attention or coordination via eye contact and joint attention
Authors: Jording Mathis , Hartz Arne , Vogel David H. V. , Schulte-Rüther Martin , Vogeley Kai
Published: April 2024

Altered nonverbal communication patterns especially with regard to gaze interactions are commonly reported for persons with autism spectrum disorder (ASD). In this study we investigate and differentiate for the first time the interplay of attention allocation, the establishment of shared focus (eye contact and joint attention) and the recognition of intentions in gaze interactions in adults with ASD compared to control persons. Participants interacted via gaze with a virtual character (VC), who they believed was controlled by another person. Participants were instructed to ascertain whether their partner was trying to interact with them. In fact, the VC was fully algorithm-controlled and showed either interactive or non-interactive gaze behavior. Participants with ASD were specifically impaired in ascertaining whether their partner was trying to interact with them or not as compared to participants without ASD whereas neither the allocation of attention nor the ability to establish a shared focus were affected. Thus, perception and production of gaze cues seem preserved while the evaluation of gaze cues appeared to be impaired. An additional exploratory analysis suggests that especially the interpretation of contingencies between the interactants’ actions are altered in ASD and should be investigated more closely.
Comprehensive fluorescence profiles of contamination-prone foods applied to the design of microcontact-printed in situ functional oligonucleotide sensors
Authors: Khan Shadman , Shakeri Amid , Monteiro Jonathan K. , Tariq Simrun , Prasad Akansha , Gu Jimmy , Filipe Carlos D. M. , Li Yingfu , Didar Tohid F.
Published: April 2024

With both foodborne illness and food spoilage detrimentally impacting human health and the economy, there is growing interest in the development of in situ sensors that offer real-time monitoring of food quality within enclosed food packages. While oligonucleotide-based fluorescent sensors have illustrated significant promise, the development of such on-food sensors requires consideration towards sensing-relevant fluorescence properties of target food products—information that has not yet been reported. To address this need, comprehensive fluorescence profiles for various contamination-prone food products are established in this study across several wavelengths and timepoints. The intensity of these food backgrounds is further contextualized to biomolecule-mediated sensing using overlaid fluorescent oligonucleotide arrays, which offer perspective towards the viability of distinct wavelengths and fluorophores for in situ food monitoring. Results show that biosensing in the Cyanine3 range is optimal for all tested foods, with the Cyanine5 range offering comparable performance with meat products specifically. Moreover, recognizing that mass fabrication of on-food sensors requires rapid and simple deposition of sensing agents onto packaging substrates, RNA-cleaving fluorescent nucleic acid probes are successfully deposited via microcontact printing for the first time. Direct incorporation onto food packaging yields cost-effective sensors with performance comparable to ones produced using conventional deposition strategies.
Jamming precoding in AF relay-aided PLC systems with multiple eavessdroppers
Authors: Kong Zhengmin , Cui Jiaxing , Ding Li , Huang Tao , Yan Shihao
Published: April 2024

Enhancing information security has become increasingly significant in the digital age. This paper investigates the concept of physical layer security (PLS) within a relay-aided power line communication (PLC) system operating over a multiple-input multiple-output (MIMO) channel based on MK model. Specifically, we examine the transmission of confidential signals between a source and a distant destination while accounting for the presence of multiple eavesdroppers, both colluding and non-colluding. We propose a two-phase jamming scheme that leverages a full-duplex (FD) amplify-and-forward (AF) relay to address this challenge. Our primary objective is to maximize the secrecy rate, which necessitates the optimization of the jamming precoding and transmitting precoding matrices at both the source and the relay while adhering to transmit power constraints. We present a formulation of this problem and demonstrate that it can be efficiently solved using an effective block coordinate descent (BCD) algorithm. Simulation results are conducted to validate the convergence and performance of the proposed algorithm. These findings confirm the effectiveness of our approach. Furthermore, the numerical analysis reveals that our proposed algorithm surpasses traditional schemes that lack jamming to achieve higher secrecy rates. As a result, the proposed algorithm offers the benefit of guaranteeing secure communications in a realistic channel model, even in scenarios involving colluding eavesdroppers.
Identification of m6A/m5C-related lncRNA signature for prediction of prognosis and immunotherapy efficacy in esophageal squamous cell carcinoma
Authors: Wang Jianlin , Ren Huiwen , Xu Chao , Yu Bo , Cai Yiling , Wang Jian , Ni Xinye
Published: April 2024

N6-methyladenosine (m6A) and 5-methylcytosine (m5C) RNA modifications have garnered significant attention in the field of epigenetic research due to their close association with human cancers. This study we focus on elucidating the expression patterns of m6A/m5C-related long non-coding RNAs (lncRNAs) in esophageal squamous cell carcinoma (ESCC) and assessing their prognostic significance and therapeutic potential. Transcriptomic profiles of ESCC were derived from public resources. m6A/m5C-related lncRNAs were obtained from TCGA using Spearman’s correlations analysis. The m6A/m5C-lncRNAs prognostic signature was selected to construct a RiskScore model for survival prediction, and their correlation with the immune microenvironment and immunotherapy response was analyzed. A total of 606 m6A/m5C-lncRNAs were screened, and ESCC cases in the TCGA cohort were stratified into three clusters, which showed significantly distinct in various clinical features and immune landscapes. A RiskScore model comprising ten m6A/m5C-lncRNAs prognostic signature were constructed and displayed good independent prediction ability in validation datasets. Patients in the low-RiskScore group had a better prognosis, a higher abundance of immune cells (CD4 + T cell, CD4 + naive T cell, class-switched memory B cell, and Treg), and enhanced expression of most immune checkpoint genes. Importantly, patients with low-RiskScore were more cline benefit from immune checkpoint inhibitor treatment (P < 0.05). Our findings underscore the potential of RiskScore system comprising ten m6A/m5C-related lncRNAs as effective biomarkers for predicting survival outcomes, characterizing the immune landscape, and assessing response to immunotherapy in ESCC.
Peroxymonosulfate enhanced photocatalytic degradation of organic dye by metal-free TpTt-COF under visible light irradiation
Authors: Xu Nong , Liu Kaixuan , Liu Qiao , Wang Qing , Zhu Anzheng , Fan Long
Published: April 2024

Recently, the activation of persulfate (PDS) by non-metallic photocatalysts under visible light has attracted significant interest in applications in environmental remediation. This study presents a pioneering investigation into the combined application of the TpTt-COF and PMS for visible light degradation of organic dyes. Synthesized orange TpTt-COF monomers exhibit exceptional crystallinity, a 2D structure, and notable stability in harsh conditions. The broad visible light absorption around a wavelength of 708 nm. The TpTt-COF emerges as a promising candidate for photocatalytic dye degradation. The study addresses high charge recombination in the TpTt-COF, highlighting the crucial role of its electron donor and acceptor for the PMS activation. Comparative analyses against traditional photocatalytic materials, such as the metal-free carbon-based material g-C3N4 and transition metal-containing TiO2, demonstrate TpTt-COF's superior performance, generating diverse free radicals. In simulated experiments, the TpTt-COF's degradation rate surpasses PMS-combined g-C3N4 by 13.9 times. and 1.6 times higher than the TpTt-COF alone. Remarkably, the TpTt-COF maintains high activity under harsh environments. Investigations into the degradation mechanism and the TpTt-COF's reusability reveal its efficiency and stability. Under visible light, TpTt-COF facilitates efficient electron–hole separation. Combining the TpTt-COF with PMS produces various radicals, ensuring effective separation and a synergistic effect. Radical quenching experiments confirm the pivotal role of O2-· radicals, while ·OH and SO4-· radicals intensify the degradation. After five cycles, TpTt-COF maintains an impressive 83.2% degradation efficiency. This study introduces an efficient photocatalytic system mediated by PMS and valuable insights into governing mechanisms for organic pollutant degradation in water environments.
A novel ternary magnetic nanobiocomposite based on tragacanth-silk fibroin hydrogel for hyperthermia and biological properties
Authors: Eivazzadeh-Keihan Reza , Mohammadi Adibeh , Aghamirza Moghim Aliabadi Hooman , Kashtiaray Amir , Bani Milad Salimi , Karimi Amir Hossein , Maleki Ali , Mahdavi Mohammad
Published: April 2024

This study involves the development of a new nanocomposite material for use in biological applications. The nanocomposite was based on tragacanth hydrogel (TG), which was formed through cross-linking of Ca2+ ions with TG polymer chains. The utilization of TG hydrogel and silk fibroin as natural compounds has enhanced the biocompatibility, biodegradability, adhesion, and cell growth properties of the nanobiocomposite. This advancement makes the nanobiocomposite suitable for various biological applications, including drug delivery, wound healing, and tissue engineering. Additionally, Fe3O4 magnetic nanoparticles were synthesized in situ within the nanocomposite to enhance its hyperthermia efficiency. The presence of hydrophilic groups in all components of the nanobiocomposite allowed for good dispersion in water, which is an important factor in increasing the effectiveness of hyperthermia cancer therapy. Hemolysis and 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assays were conducted to evaluate the safety and efficacy of the nanobiocomposite for in-vivo applications. Results showed that even at high concentrations, the nanobiocomposite had minimal hemolytic effects. Finally, the hyperthermia application of the hybrid scaffold was evaluated, with a maximum SAR value of 41.2 W/g measured in the first interval.
Analyzing the dynamical sensitivity and soliton solutions of time-fractional Schrödinger model with Beta derivative
Authors: Nadeem Muhammad , Liu Fenglian , Alsayaad Yahya
Published: April 2024

In physical domains, Beta derivatives are necessary to comprehend wave propagation across various nonlinear models. In this research work, the modified Sardar sub-equation approach is employed to find the soliton solutions of (1+1)-dimensional time-fractional coupled nonlinear Schrödinger model with Beta fractional derivative. These models are fundamental in real-world applications such as control systems, processing of signals, and fiber optic networks. By using this strategy, we are able to obtain various unique optical solutions, including combo, dark, bright, periodic, singular, and rational wave solutions. In addition, We address the sensitivity analysis of the proposed model to investigate the truth that it is extremely sensitive. These studies are novel and have not been performed before in relation to the nonlinear dynamic features of these solutions. We show these behaviors in 2-D, contour 3-D structures across the associated physical characteristics. Our results demonstrate that the proposed approach offers useful results for producing solutions of nonlinear fractional models in application of mathematics and wave propagation in fiber optics.