bluuebunny/crossref_metadata_2025 huggingface dataset:
Determinants of the electronic distribution of services to tourism organizations in the Kingdom of Saudi Arabia
Authors: Al-Hazmi Nabil Mohemmed
Published: October 2020

This study aims to determine the determinants of the electronic distribution of services for tourism and travel organizations in the Kingdom of Saudi Arabia. The study uses some descriptive analytical approach in achieving its goals and testing its hypotheses. The study found that there are a set of determinants that stand for the distribution of services of tourist organizations, the most important of which is the weakness of communication and software networks as well as the lack of qualified human resources to manage distribution channels. The study recommends the need to develop communication networks and train human resources to manage electronic distribution channels.
The effects of environmental knowledge and green products awareness on green management and sustainable performance: Evidence from manufacturing sector in UAE
Authors: Khaleeli Majdi , Pradeep Suja , Krishnadas Latha
Published: October 2020

This research intends to figure the antecedents of Green Management and its implementation in manufacturing sector companies in UAE. The study also intends to explore the relationship between the implementation of green management and sustainable performance as measured by complied scale of social, economic and environmental performance. Quantitative research methods are used and the necessary data are collected from 134 companies by using google form survey sent to participants. Statistical Package of Social Sciences (SPSS) Version 22 is used to analyze the data. The findings of this research reveal that managers’ environmental knowledge and managers’ green product awareness were positively and significantly associated with green management and that green management was also positively and significantly associated with sustainable performance.
The role of parents on their children’s academic performance
Authors: Nguyen Lan , Duong Nga , Dinh Huyen , Nguyen Mai , Nguyen Thoa
Published: October 2020

This study aims to investigate the impact of the family factors on scholastic achievements of students in high schools and universities in Vietnam. The primary data is collected from the 538 students through an online questionnaire. We apply one empirical model with the support of SPSS and STATA to test the family determinants of academic attainments-GPA. We find that there are three factors which impact on GPA of these: Age gap, Income of the families, Negative reactions of parents to their child’s low-performed results in academic performance. The research results show that all three factors can explain for 8% with the changes in GPA of learners. While the age gap between parents and their children has minor positive effects on academic performances, family income is significantly and positively correlated to GPA. This is the reason why students from opulent families often perform better than those of more meager families.
Effect of strength of the external environment and Islamic business ethics on the performance of Islamic finance companies with intellectual capital mediation
Authors: Hasbi Hariandy , Moeljadi Moeljadi , Noermijati Noermijati , Rofiq Ainur
Published: October 2020

The purpose of this study is to analyze sharia microfinance strategies to encourage the economy at the community level such as the Integrated Business Centers (BMT) based on Sharia principles. Primary data were obtained from questionnaires on 283 BMT managers in Java. Using PLS analysis, strengthening corporate performance is measured by Islamic business ethics with intellectual capital as mediation. This model construct is unique and provides value in this study. The results showed that the strength of the external environment did not directly affect the performance of the BMT; Islamic business ethics and intellectual capital have positive and significant effects on BMT performance; and Intellectual capital is proven to mediate the indirect effect of the strength of the external environment and Islamic business ethics on BMT performance. Islamic Business Ethics in its application greatly supports the vision and mission of BMT. With the good implementation of Islamic Business Ethics, the level of customer confidence from BMT increases and will automatically increase BMT performance. Strength of the External Environment able to influence BMT performance after mediation from the Intellectual Capital. Intellectual Capital owned by BMT employees is an inseparable part and greatly supports BMT performance. This is evident in this study in which qualified intellectual capital from the employees had a good impact on improving BMT performance.
Novel models for obtaining the closest weak and strong efficient projections in data envelopment analysis
Authors: Vakili Javad , Amirmoshiri Hanieh , Mirnia Mir Kamal
Published: October 2020

Data Envelopment Analysis (DEA) is a nonparametric method for measuring the relative efficiency and performance of Decision Making Units (DMUs). Traditionally, there are two issues regarding the DEA simultaneously i.e., the identification of a reference point on the efficient boundary of the production possibility set (PPS) and the use of some measures of distance from the unit under assessment to the efficient frontier. Due to its importance, in this paper, two alternative target setting models were developed to allow for lowefficient DMUs find the easiest way to improve its efficiency and reach to the efficient boundary. One seeks the closest weak efficient projection and the other suggests the most appropriate direction towards the strong efficient frontier surface. Both of these models provides the closest projection in one stage. Finally, a proposed problem is empirically checked by using a recent data related to 30 European airports.
On the solution of an f(r) theory of gravity motivated by gravitational waves in spherically symmetric space-time
Authors: Mishra Aditya Mani
Published: October 2020

There are many modification of Einstein theory have been established which explain the behavior of universe realistically or hypo-theoretically. Out of those, an f(R) theory of gravity based on non-conformal invariance of gravitational waves has been developed. We attempted to find the solution of this theory for spherically symmetric spacetime in vacuum and compared its result to Einstein theory. We have concluded that solution is consistent with Newtonian limit at large distance from source. Solution predicts two horizon in the spacetime, none of them coincides with Schwarzschild counterpart. However, as this f(R) theory converges to Einstein theory, these horizons coincide.
Conceptismo, epigramatismo y devoción pública en la Sevilla inmaculista: una aproximación al Escuadrón humilde (1616) de Rodrigo Fernández de RiberaConceptism, Epigramatism and Public Devotion in Immaculist Seville: An Approach to Rodrigo Fernández de Ribera’s Escuadrón Humilde (1616)
Authors: Daza Juan Manuel
Published: October 2020

The immaculist movement was one of the most suggestive episodes of the Baroque Seville. Literature, and especially poetry, shows the importance given to the arts in the development of this religious and sociological phenomenon, as can be seen in Rodrigo Fernández de Ribera’s work Escuadrón humilde (humble squad). This work evinces how poetry was not only a festive and apologetic instrument of the faith, but how it could also become a divulgative vehicle with a great doctrinal charge of the ideas that were being debated in literary circles.
El Encomio de los ingenios sevillanos, en la fiesta de los santos Inacio de Loyola y Francisco Javier (1623), de Juan Antonio de Ibarra
Authors: Osuna Inmaculada
Published: October 2020

Encomio de los ingenios sevillanos is a collection of poems from a poetic contest honoring Saint Ignatius of Loyola and Saint Francis Xavier, promoted in Seville by an influential corporation in the commercial field. Juan Antonio de Ibarra’s presentation of the poems in this collection has several meta-literary considerations. The celebration of the contest by the initiative of this civil corporation, several editorial details in the book or its author’s literary links and interests allows us to contextualize the prominence of this meta-literary component and its main purpose: the exaltation of current local poets, continuing with the brilliant trajectory in Seville.
Development of a qPCR to diagnose the genus Eimeria in bovines
Authors:   Universidade Norte do Paraná, Cardim Sérgio Tosi , Cardim Stefani Lino ,   Universidade Estadual de Londrina, Seixas Mércia de ,   Universidade Norte do Paraná, Taroda Alessandra ,   Universidade Estadual de Londrina, Carneiro Priscilla Gomes ,   Universidade Estadual de Londrina, Zulpo Dauton Luiz ,   Pontifícia Universidade Católica do Paraná, Barros Luiz Daniel de ,   Universidade Estadual de Londrina, Bortolloti Guilherme Luis ,   Universidade Estadual de Londrina, Garcia João Luis ,   Universidade Estadual de Londrina
Published: October 2020

Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, the 18S rRNA region of the DNA of these parasites was selected, since it is a region with low variability among the species. The qPCR was developed using the SYBR Green, resulting in a PCR with a high sensitivity, able to amplify samples containing only one oocyst of Eimeria spp. of bovines. The feasibility of using qPCR in the diagnosis of the Eimeria Genus is demonstrated in this study, once this technique shows to be less laborious and needs less skills for diagnostic training when compared to the technique conventionally used in theroutine (micromorphometry).
Equivalence in Bilingual Dictionaries
Authors: Yu Xiaomei
Published: October 2020

The task of the bilingual dictionary is to provide lexical units in the source language with equivalents in the target language. Therefore, translation equivalence is of great importance in compiling a bilingual dictionary. This study is an introduction to categories of equivalence in bilingual dictionaries and the causes of non-equivalence. Some methods for translating non-equivalence are also recommended.
ELT During Lockdown: A New Frontier in Online Learning in the Saudi Context
Authors: Hashmi Uzma M. , Rajab Hussam , Shah Sayyed Rashid Ali
Published: October 2020

This study explores pedagogical challenges pertaining to the new online English Language Teaching (ELT) practices that emerged due to the covid-19 pandemic outbreak and the subsequent worldwide lockdown. Based on an explanatory sequential, mixed methods, descriptive research design, quantitative and qualitative data from 265 English as a Foreign Language (EFL) teachers in the Saudi context were collected by utilising a custom designed, twenty-two items on a psychometric five-point Likert items, open-ended questions, and a questionnaire. The quantitative data were statistically analysed using SPSS whereas the qualitative textual data were analysed employing the grounded theory. The findings of the study indicate that EFL teachers regard full scale online teaching as a challenging endeavour; however, a valuable and indispensable tool in language teaching, especially, during the outbreak of covid-19 pandemic. Furthermore, most of the participants prefer to receive more professional development opportunities based on real life teaching experiences in online ELT. The study presents suggestions and recommendations for further research.
Online Messages Sentiments Analysis Based on Long Short-Term Memory
Authors: Zhao Yunke
Published: October 2020

In December of 2019, an extremely infectious and deadly pandemic ambushed China. In Wuhan, the novel coronavirus COVID-19 suddenly broke out and spread rapidly to other countries. COVID-19 became a worldwide disaster, affecting not only physical, but also emotional health on a global scale. We wanted to record this change based on the sentiment analysis model and to examine the relationship between world events and the positivity of posts on social media.

To analyze this relationship, we utilized a set of movie reviews as a training sample to construct a sentiment analysis model based on the Long Short-Term Memory neural network theory, and calculate the texts' sentiment score. We then analyzed the overall trend of the data, and discussed the reason behind the tendency. The principal result was that, as the pandemic progressed, online sentiment generally became more positive. We believe that this is because people gradually become more accustomed to life in the COVID-19 era.
Reviewer Acknowledgements for Public Administration Research, Vol. 9, No. 2
Authors: Tai Gabriel
Published: October 2020

Reviewer acknowledgements for Public Administration Research, Vol. 9, No. 2, 2020.
When poetry becomes autobiography: anecdote as an interpretative tool in the Greek classical epoch
Authors: Skarbek-Kazanecki Jan
Published: October 2020

The article discusses the role of biography in the reception of archaic poetry in the classical period. As it is illustrated by a fragment of Critias (295W), in the fi fth century B.C. the archaic poetic traditions, previously transmitted orally through performance, began to be interpreted from a biographical perspective: fi rst-person statements were mostly associated with the poets themselves and treated as a source of biographical information; in other words, archaic poetry came to be seen as a kind of autobiography. Anecdotes about poets were used to interpret the same poems which had provided the basis for these false stories: as an interpretative tool, they simplifi ed old compositions, not always clear for the reader. Until the 1980s, classical philologists often relied on false testimonies from the classical and Hellenistic era, limited by their attachment to the biographical perspective.
Autobiographism – seeing and understanding. A few remarks on the poetry of Erna Rosenstein
Authors: Grodź Iwona
Published: October 2020

The article analyzes selected literary works by Erna Rosenstein (1913–2004) through an autobiographical lens. Rosenstein’s poems can be interpreted as emotional and sensual accounts of traumatic experiences from the time of the Second World War.
Instagram as a form of personal journal. The case of Banksy
Authors: Michalina Lubaszewska
Published: October 2020

Banksy, the world’s most famous graffi ti artist, has never revealed his identity. He communicates with the public via his website and Instagram profi le. On Instagram the artist posts the pictures of his works. That’s his way to confi rm their authenticity, and sometimes, if there is a need, to comment on phenomena related to his work. Banksy’s Instagram account is an element of his artistic auto-creation. His posts are very rare and laconic, building up the vibe of secretiveness. Nevertheless his Instagram narrative can be analyzed as a personal journal; according to Philippe Lejeune, the structure of a personal diary is based on what is hidden and not written. The article treat Banksy’s posts on Instagram as a „cycle of dated marks”.
A moufl on is waiting on the doorstep. Cosima by Grazia Deledda as a literary transformation of a biography
Authors: Przybysz Iwona
Published: October 2020

The article analyzes the methods of a literary transformation of the biography of Grazia Deledda in her unfi nished novel Cosima (editio posthuma 1937). This novel marks an important attempt to expand the autobiographical context of Grazia Deledda’s literary output insofar as, in generic terms, it represents „a novel of transformation” (analogous to Bildungsroman but presented from a woman’s perspective). Deledda’s method of rewriting a biographical narrative consists employing some elements of a fairy tale, with the moufl on as an allegory of the fate of a woman who tries to compromise her desire of artistic freedom and obligation to fulfi l gender roles. Thus literary biography of the novel’s protagonist (and also of Grazia Deledda herself) establishes a paradigm of fight as an expression artistic freedom in the face of various forms of cultural imposition.
EDUCATION AND THE LABOUR MARKET IN POLAND – SELECTED ISSUES
Authors: Buchowicz Izabela
Published: October 2020

Education is considered as an important factor of well-prosperous economy. Education which corresponds to the needs of a labour market becomes the key educational target of young people and employees who want to adopt to the changes at the labour market. Therefore there are still challenges for education in Poland – how to modify the process of teaching and how to infl uence the process of learning in such a way that education would become an important factor in growth of human and social capital. Contemporary education should take into account variable requirements of the labour market, including also needs of the present and future employees and employers. An important challenge for education in Poland is to adopt its structure and teaching programs to the needs of employees not only on the domestic labor market, by also on the labour markets of other countries, mainly in Europe. Simultaneously, Polish educational system must be competitive with respect to those of other European countries, take into account demographic and technological changes in order to prepare employees willing to work in diff erent countries.
AGEING AND OLD AGE IN RESEARCHES OF THE INSTITUTE OF SOCIAL ECONOMY
Authors: Błędowski Piotr
Published: October 2020

Aging and old age are becoming increasingly important topics of scientific research and publications. Social and economic conditions and the consequences of old age are getting more and more important for social policy. The Institute of Social Economy (IGS) has been dealing with this issue since the 60s of the last century, when the IGS team conducted the first representative nationwide survey of the social and economic living conditions of elderly people. Later, employees and co-workers of the Institute managed and participated in many nationwide researches and conducted a number of scientific projects in gerontology. The subject of scientific works concerned mainly the living conditions of elderly people and social policy towards elderly people as well as organization and financing of the Long-Term Care. The aim of this paper is to present the main directions of scientific work of the Institute of Social Economy in the field of social gerontology and the description of its scientific achievements in this field.
MamuLEDs: Mixed Reality meets Mamulengo
Authors: Jácome Jarbas , Oliveira Maria , Alvim Fernando , Teichrieb Veronica , Ramalho Geber
Published: October 2020

Techniques of Virtual, Augmented, and Mixed Reality (VR, AR, MR) have been used for puppet theater in different cultural contexts around the world. However, we are not yet aware of the use of these techniques in the Brazilian Northeast Popular Puppet Theater, a tradition known in Pernambuco as Mamulengo. We present here a system developed for “The Quarrel Between the Real Puppet and the Virtual Puppet,” a sketch created for the class Realidade Virtual e Aumentada of Computer Science program at CIn-UFPE. This paper aims to investigate how VR/AR/MR techniques can contribute to the creation of mamulengo shows. We used the method of qualitative case study, analyzing the audiovisual records of essays and presentation, the software solution developed in Unity 3D, and the data collected from a focus group interview with puppeteers. This study identified as weaknesses of our system: the hand tracking unreliability and the lack of tactile feedback, causing discomfort in experienced puppeteering professionals. As strengthens of the system, we found that the technology aroused interest because of its potential for expanding the options of tools available for popular puppetry shows creation in current times.
The Authentic Meaning of Free Speech
Authors: Vacca Michael Arthur ,   The National Catholic Bioethics Center
Published: October 2020

This article examines a common misconception regarding the scope of free speech in the context of abortion counseling. Specifically, a recent critique of pro-life legislation identifies an apparent contradiction between (1) requiring doctors to show an ultrasound and play a fetal heartbeat to a mother before providing her with an abortion and (2) not requiring crisis pregnancy centers to give women information on where they can procure an abortion. Giving a patient comprehensive information about the effects of a medical procedure (i.e., the death of the fetus) is a necessary element of obtaining informed consent. A pro-life intention behind such regulation neither invalidates the scientific relevance of the information nor forces a physician to provide medical advice with which he or she disagrees. In contrast, requiring a crisis pregnancy center to provide information on abortion resources changes the organization’s message to advertise practices it morally opposes.
A Guide to Palliative Care and Hospice
Authors: Zalot Jozef ,   The National Catholic Bioethics Center
Published: October 2020

Palliative care and hospice can be confusing subjects. Many people misunderstand the meaning of both and often consider the two terms synonymous. There are, however, important differences between palliative care and hospice. Palliative care is designed to anticipate, prevent, and manage physical, psychological, social, and spiritual suffering. It is primarily concerned with the symptoms that result from an illness or from the interventions used to treat said illness. It includes both the terminally ill and those with chronic, life-limiting conditions. Hospice is a type of palliative care provided to individuals with a life expectancy of six months or less. Patients are generally admitted to hospice when their condition no longer responds to aggressive interventions or they have chosen to discontinue medical intervention for ethically appropriate reasons. It is necessary to approach enrolling in hospice with caution, and important questions must be considered before doing so.
The Spreading Cancer of Counterfeit Drugs
Authors: Pitts Peter
Published: October 2020

Just as the coronavirus mutates to survive and thrive, so to do the purveyors of counterfeit medicines – with their high-speed “host” being the digitization of patient care. The future is now. So, how do we balance moving forward with user-friendly digitization, telemedicine and virtual healthcare delivery while simultaneously recognizing the unintended consequences of the innovative criminal mind? The first step is to recognize there’s a problem.
Analysis of means and methods of stress-strain state control of structures during operation
Authors: Devyatov Ivan , Iurgenson Sergei , Zharenov Ivan , Trutnenko Andrei , Tuev Dmitrii
Published: October 2020

This article defines the basic set of means and methods of the structural health monitoring of metal structures and structures from polymer composite materials (PCMs) used in the construction and aviation industry. The analysis of means and methods of strain-stress state control of a structure during operation (detection of defects and crack growth) and their comparison is provided.
The â€˜decoadaâ€™ phenomenon effect on the Paraguay river water quality, Northern Pantanal
Authors: Menezes Junior Mauri Queiroz de , Oliveira Junior Ernandes Sobreira , Souza Célia Alves de
Published: October 2020

In the Pantanal, during the beginning of the flooding hydrological phase occurs a natural phenomenon called 'decoada'. Characterized by the water quality alteration of the rivers, in floodplain areas and marginal lagoons, with the possibility of significant fish mortality, depending on the changes’ magnitude. For this purpose, throughout a hydrological cycle (July 2018 to June 2019), precipitation, bathymetry and water quality were monitored monthly, in 6 sampling points, to determine, the moment, duration and intensity of the decoada, and its effects on water quality in the Paraguay River, northern Pantanal, near the municipality of Cáceres/MT. The dissolved oxygen (DO) concentration was used as an indicator, in which DO < 3.0 mg/L, during the flood season, was considered indicative of the potential event occurrence. According to the records, there were different hydrological periods, where the flooding and high waters presented DO values lower than in the dry and ebb phases, with a drastic change from October 2018 (DO = 6.37 ± 0.17 mg/L) to November 2018 (DO = 2.78 ± 0.05 mg/L), registering, therefore, in all points, low-intensity decoada. The DO values remained below 5 mg/L, the maximum limit allowed by CONAMA Resolution 357/2005 for class II rivers. From November 2018 to April 2019 in all sampling points, the minimum value was recorded in March 2019 in the bay, with DO = 1.84 mg/L, considered a medium level decoada. The principal component analysis (PCA), proved the results difference of decoada found in the river and the bay, presented with greater intensity and duration in the bay. The parameters that influenced the decoada, according to the PCA, were pH, temperature, precipitation, turbidity and DO.
Automation of choosing hot and cold pixels process in the estimate of the sensitive heat flow and evapotranspiration
Authors: Danelichen Victor Hugo de Morais , Gomes Raphael de Souza Rosa , Figueiredo Josiel Maimone de , Velasque Maísa Caldas Souza , Machado Nadja Gomes , Nogueira José de Souza , Biudes Marcelo Sacardi
Published: October 2020

Characterize accurately and accurate evapotranspiration (ET) on a global scale has been one of the major determinant challenges in terrestrial ecosystem studies, climate dynamics and hydrological cycle. The goal in this study was to evaluate methodologies presented in the literature proposing a new methodology and automation in the process of selection of hot and cold pixels to need the sensible heat flux H and ET. The study was conducted in four different locations in the state of Mato Grosso, Brazil. And two areas of forest and two formed by pasture. For the H automation process we used the proposed Bastiaanssen et al. (1998a), Gao et al. (2011), and other vegetation using different levels of the literature suggests. The automation of the selection of hot and cold pixels process was successful, showing correlations above 0.83 for the estimates of H and 0.75 for estimates of ET. The identification of hot and cold pixels using the methodology by similarity of neighboring pixels using the average of pixels showed the highest correlations to the H in the study areas. LAI had the best indicator in the automated identification of hot and cold pixels to estimate the daily evapotranspiration (ET24h).
Type of temperament as a factor of formation of marriage structure of a population
Authors: Etkalo E. N. , Atramentova L. A.
Published: October 2020

Aim. To clarify the role of temperament in the formation of the marriage structure of the urbanized population. Methods. The type of temperament was determined using the G. Eysenck personality questionnaire (EPI). Results. 267 couples were examined. The age of men is 35-57 years old, the age of women is 33-55 years old. The entire survey was Kharkov residents - ethnic Ukrainians and Russians. Among the studied marriages, 105 (almost 40%) were endogamous in relation to temperament, 162 (60%) were exogamous. The actual number of endogamous marriages was greater, and exogamous less than the theoretically expected number in panmixia. All endogamous marriages turned out to be positively assortative, the indicator of marital assortativeness for various types of temperament was 17-25%. Conclusions. In endogamous marriages, only the effect of “attraction” was observed. In exogamous marriages, both statistical “attraction” and “repulsion” of temperaments are noted. The effect of "attraction/ repulsion" manifests itself in different ways in reciprocal marriages. Keywords: temperament, population, endogamy.
Genetic profiling of mistletoe (Viscum album L.) using RAPD-analysis
Authors: Bilonozhko Yu. O. , Rabokon A. N. , PostovoitovA A. S. , Kalafat L. O. , PrivAlikhin S. M. , Pirko N.M. , Demkovych A. Ye. , Blume Ya. B. , Pirko Ya. V.
Published: October 2020

Aim. The aim of this research was genetic profiling and identification of genetic differences between V. album speciments, growing on deciduous and coniferous species of woody plants using RAPD markers. Methods. The method of polymerase chain reaction (PCR) with random primers (Random Amplification of Polymorphic DNA - RAPD) was used. Amplified DNA fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using the staining with silver nitrate. Results. All the studied mistletoe samples were differentiated from each other, and their unique molecular genetic profiles were obtained. 241 amplified DNA fragments were detected in the range from 200 to 2000 bp, 152 fragments (63%) were polymorphic. The samples were divided into two separate groups depending on the type of host plant. Conclusions. The fact that the samples formed two separate clades confirms the assumption that mistletoe, which grow on pine and grow on maple, represents two separate subspecies of V. album.

Keywords: Viscum album L., molecular genetic markers, polymorphism, RAPD.
Optimization of Agrobacterium-mediated transformation of perspective winter wheat genotypes in vitro
Authors: Dubrovna O. V. , Slivka L. V.
Published: October 2020

Aim. Optimization of conditions for genetic transformation of new perspective winter wheat genotypes. Methods. Agrobacterium-mediated transformation in culture in vitro. Results. The influence of the optical density of cells of the agrobacterial suspension, the concentration of the antibiotic cefotaxime, the duration of coculture on the frequency of obtaining kanamycin-resistant regenerants of new winter wheat genotypes by genetic transformation of callus cultures were investigated using LBA4404 and AGL0 strains. It is shown that depending on the strain the most optimal is the concentration of agrobacteria 0.2-03 OD, duration of coculture for 2-3 days and the use of cefotaxime at a concentration of 250-500 mg/L. Conclusions. The optimal parameters for conducting Agrobacterium-mediated transformation of callus cultures of new perspective winter wheat genotypes were selected.

Keywords: Triticum aestivum, Agrobacterium-mediated transformation, callus cultures.
Genetic and physiological analysis T1 biotechnological plants of winter wheat (Triticum aestivum L.)
Authors: Komisarenko A. G. , Mykhalska S. I. , Kurchii V. M. , Khrystan O. O.
Published: October 2020

Aim. To investigate inheritance of transgenes in the first generation (T1) of winter wheat biotechnological plants (Triticum aestivum L.). Analyze the performance of T1 genetically modified plants with a double stranded RNA suppressor of the proline dehydrogenase (pdh) gene under normal growing conditions. Methods. PCR analysis, DNA electrophoresis; determination of indicators of the structure of the crop. Results. Molecular genetic analysis was performed and the performance indicators of control and T1 biotechnological plants were investigated. Conclusions. The first generation of genetically modified winter wheat plants resulting from Agrobacterium-mediated transformation in planta confirmed the inheritance of integrated genes. Among the transgenic variants identified plants that lack some fragments of the target gene required for partial suppression of the gene of proline dehydrogenase wheat. It is shown that at the optimal terms of growing biotechnological plants of wheat winter-annual of UK 322/17 and UK 209 h was characterized by the best indexes of structure of harvest as compared to an initial form, while the genetically changed plants of genotypes of UK 95/17 and UK 065 after the elements of the productivity did not differ from control plants.

Keywords: Triticum aestivum L., biotechnological plants, T-DNA, proline dehydrogenase gene, structural analysis indicators.
Tissues of immature and mature embryos as morphogenic competent explants for genetic transformation of wheat
Authors: Mykhalska S. I. , Komisarenko A. G. , Kurchii V. M.
Published: October 2020

Aim. To analyze the efficiency of using tissues of immature and mature embryos for Agrobacterium-mediated transformation in vitro of new breeding-valuable genotypes of winter wheat (Triticum aestivum L.) for the purpose of their genetic improvement. Methods. Culture of in vitro, extraction and electrophoresis of DNA, PCR analysis. Results. The efficiency of induction of callusogenesis and regeneration of winter wheat shoots was analyzed. The morphogenetic response of callus cultures obtained from different explants under Agrobacterium-mediated transformation in vitro was investigated. Molecular genetic analysis of wheat regenerants for transgenes was performe. Conclusions. The tissues of immature and mature embryos of novel breeding-valuable wheat genotypes are competent explants for Agrobacterium-mediated transformation in vitro. In this case, the tissues of two daily sprouts of mature wheat germ are characterized by higher morphogenetic parameters, which helps to obtain a greater percentage of genetically modified variants.

Keywords: Triticum aestivum L., in vitro, Agrobacterium-mediated transformation, immature embryos, mature embryos.
Combined space-organized system for animals oocytes maturation in vitro
Authors: Syrvatka V. J. , Slyvchuk Yu. I. , Shtapenko O. V. , Gromyko O. M. , Gevkan I. I.
Published: October 2020

Aim. The aim of the work was to create a space-organized system for oocytes maturation based on rabbits granulosa cells, hyaluronic acid (for structural organization of this system) and insulin, as an important growth factor. Methods. Cultivation of granulosa cells and oocytes maturation in vitro, biochemical determination of lactate dehydrogenase activity and cholesterol concentration, and determination of progesterone and estradiol concentrations by enzyme immunoassay were done. Results. Hyaluronic acid in concentration of 0.5 μg/ml increased proliferation of granulosa cells and progesterone concentration in the conditioned medium on 72 h of cultivation, and estradiol on 48 and 72 hours of cultivation was found. The degree of oocyte maturation to metaphase-2 was 85% by the influence of 10 μg/ml insulin. Same concentration of insulin increased granulosa cells proliferation, estradiol, progesterone and cholesterol concentration in the conditioning medium after 24 hours of cultivation. Conclusions. The optimal parameters of the combined spaceorganized culture system are 1 million cells/ml of granulose with the addition of 0.5 μg/ml hyaluronic acid and 10 μg/ml insulin, which creates the optimal conditions for the maturation of oocytes to metaphase-2 cell cycle.

Keywords: oocytes, granulosa cells, hyaluronic acid, insulin, in vitro.
The application of microbial originating bioregulators to obtain in vitro lines of potato with increased resistance to parasitic nematodes
Authors: Shysha O. M. , Spivak S. I. , Tsygankova V. A. , Iutynska G. O. , Biliavska L. O. , Yemets A. I. , Blume Ya. B.
Published: October 2020

Aim. Obtaining in vitro new lines of potato (Solanum tuberosum L.) cultivar Vernissage with genetically mediated resistance to the parasitic nematode H. schachtii on nutrient MS media with microbial bioregulators. Methods. In vitro conditions the process of organogenesis of potato plants on nutrient MS media containing microbial bioregulators used at concentrations 25-100 μl/l in combination with phytohormones 2 mg/l BAP and 0,1 mg/l NAA was investigated. Using dot blotting method the degree of hybridization between cytoplasmic si/miRNA isolated from cells of potato plants-regenerants, grown on the artificial invasive background, and nematode mRNA was studied. In the wheat seedlings cell-free system in vitro the silencing activity of si/miRNA isolated from cells of potato plants-regenerants on the template of nematode mRNA was investigated. Results. The experiments conducted in vitro conditions showed that the addition of microbial bioregulators at concentrations 25-100 μl/l in combination with phytohormones 2 mg/l BAP and 0,1 mg/l NAA into MS media increased the efficiency of regeneration of potato shoots to 43–47 % as compared with similar indices obtained on control MS media. The increase of the degree of hybridization to 19-38 % between cytoplasmic mRNA isolated from cells of nematode H. schachtii and si/miRNA isolated from cells of experimental potato plants-regenerants grown in vitro conditions on nutrient media containing bioregulators on the artificial invasive background was shown. Conclusions. Using microbial bioregulators in vitro conditions as components of nutrient MS medium increases potato shoot regeneration efficiency and enhances RNAi-mediated resistance of plants-regenerants to parasitic nematode H. schachtii.

Keywords: microbial bioregulators, potato organogenesis in vitro, potato resistance to nematode Heterodera schachtii, RNA interference.
Alanine scanning of dinitroaniline/phosphorothioamidate site of α-tubulin in plasmodium species distributed in India
Authors: Demchuk O. M. , Karpov P. A. , Rayevsky A. V. , Ozheredov S. P. , Spivak S. I. , Yemets A. I. , Blume Ya. B.
Published: October 2020

Aim. Identification of amino acid residues participating in specific binding of dinitroaniline and phosphorothioamidate compounds with α-tubulin in Plasmodium falciparum. Methods. Protein structure modelling, protein structure optimization using molecular dynamics method, ligand-protein docking, alanine scanning mutagenesis. Results. Molecular docking of canonical compounds and alanine scanning mutagenesis, indicate two key (Arg2, Val250) and one minor (Glu3) residues involved in binding of both - dinitroaniline and phosphorothioamidate compounds. At the same time, it was revealed two minor residues (Asp251, Glu254) interacting only with some members of dinitroaniline grope. Conclusions. It was identified amino acid residues predetermining existence of joint site and similar interaction of α-tubulin with dinitroani-line and phosphorothioamidate compounds in P. falciparum.

Keywords: malaria, Plasmodium, α-tubulin, molecular interaction, dinitroanilines compounds, phosphorothioamidate compounds, alanine scanning mutagenesis.
Outstanding scientist and bacteriologist Waldemar Haffkine
Authors: Chen I. B. , Humeniuk H. B.
Published: October 2020

Waldemar Haffkine is an outstanding bacteriologist, immunologist and epidemiologist who was born in Ukraine. He studied at the Department of Natural Sciences at the Imperial Novorossiisk University (now Odessa I.I. Mechnikov National University), and his scientific career as a zoologist began under the guidance of the Nobel Prize for Physiology or Medicine Ilia Mechnikov. Working at the Pasteur Institute, Paris, he developed a vaccine against cholera, tested its effectiveness on himself and for the first time vaccinated people against bacterial diseases. During the cholera epidemic in India, he established a vaccine production, organized preventive vaccinations and inoculated tens of thousands of people, as a result of which morbidity and mortality decreased tenfold. When the plague epidemic struck Bombay, W. Haffkine soon developed a plague vaccine and re-tested its safety. He founded a bacteriological laboratory in Bombay for the production of vaccines and organized large-scale vaccination schemes. The Haffkine Institute still makes millions of doses of vaccines and serums, saving people from cholera, plague, typhus, rabies, tetanus and other diseases.

Keywords: anticholera vaccine, antiplague vaccination, inoculation schemes, the Haffkine Institute.
Litterær faglighet i en samtale om Roy Jacobsens novelle ”Brønnen”
Authors: Hennig Åsmund
Published: October 2020

Artikkelens formål er å undersøke hva slags litterær faglighet som kommer til uttrykk i en elevstyrt litterær samtale om en krevende novelle, samt å undersøke hvilke vekstpunkter samtalen inneholder. Artikkelen vil også drøfte hvordan observert faglighet og vekstpunkter kan kommuniseres i formativ vurdering. Bakgrunn for artikkelen er blant annet et ønske om bedre å forstå substansen i den litterære fagligheten til en spesifikk elev som ofte viste stor faglighet i litterære samtaler, samt å utvikle mer presis formativ vurdering for videre vekst. Det empiriske grunnlaget for artikkelen er en analyse av lydopptak av gruppens samtale om teksten og av en samtale der gruppen vurderer sin egen litterære samtale. Det teoretiske grunnlaget består av transaksjonsteori, teori om litterære samtaler og utforskende dialog og teori som drøfter begrepene litterær kompetanse og litterær faglighet. Analysens sentrale funn, basert på presenterte og drøftede analysekategorier, er at gruppen viser stor utholdenhet og et intellektuelt ønske om å forstå bedre, og at samtalen inneholder vesentlige innslag av utforskende dialog, drevet fram av særlig den ene elevenes litterære faglighet. Vekstpunkter beskrives av begreper som oppfølging og dveling, samtidig som det i en formativ vurdering blir viktig å gjøre elevene bevisste på egen litterær faglighets omfang og betydning.
A systematic review of research on how students and teachers use didactic learning materials in L1
Authors: Gissel Stig Toke , Buch Bettina
Published: October 2020

This article presents the results of a systematic review of research on how students and teachers in primary and lower secondary school use didactic learning materials in L1. Didactic learning materials are learning resources made for purposes of teaching and learning. We include both digital and analogue learning resources and methodically diverse research published 2008-2018. The aims of the review is mapping 1) how much research there is in this field, 2) which aspects of use are investigated, 3) what the research can tell us about the use of didactic learning materials, and 4) how the research was conducted methodically. We synthesize the included studies in six themes that describe strands of research interests. Finally, we will propose a research agenda for moving the field of research in the use of didactic learning materials forward.
Les stratégies d’accueil et d’accompagnement des nouveaux arrivants déployées par les écoles francophones et les structures communautaires du Nouveau-Brunswick
Authors: Kamano Lamine , Benimmas Aïcha , Côté Alexandra
Published: October 2020

L’accueil et l’accompagnement des nouveaux arrivants dans leur processus d’établissement constituent l’une des préoccupations des acteurs sociaux et éducatifs de la province du Nouveau-Brunswick. Cet article présente les résultats d’une étude qui vise à comprendre les stratégies d’accueil, de sensibilisation et d’accompagnement déployées par les écoles francophones et les structures d’accueil pour intégrer les familles issues de l’immigration et leurs enfants ainsi que les enjeux entourant le partenariat possible entre ces deux sphères en matière d’intégration des personnes immigrantes. Pour ce faire, une série d’entrevues individuelles ont été menées auprès des directions d’école, du personnel enseignant, des membres d’organismes communautaires et des parents immigrants. L’analyse thématique des données recueillies a permis de dresser un portrait global des différentes stratégies mises en oeuvre par les écoles et les organismes non gouvernementaux (ONG) et communautaires en vue d’accompagner les élèves nouveaux arrivants et leurs familles. Les résultats montrent plusieurs éléments de satisfaction découlant de la collaboration entre les écoles et les organismes communautaires. Toutefois, l’appréciation de ce partenariat tacite par les directions d’écoles et les parents immigrants laisse voir plusieurs lacunes à combler.
Lipid hijacking: A unifying theme in vector-borne diseases
Authors: O'Neal Anya J , Butler L Rainer , Rolandelli Agustin , Gilk Stacey D , Pedra Joao HF
Published: October 2020

Vector-borne illnesses comprise a significant portion of human maladies, representing 17% of global infections. Transmission of vector-borne pathogens to mammals primarily occurs by hematophagous arthropods. It is speculated that blood may provide a unique environment that aids in the replication and pathogenesis of these microbes. Lipids and their derivatives are one component enriched in blood and are essential for microbial survival. For instance, the malarial parasitePlasmodium falciparumand the Lyme disease spirocheteBorrelia burgdorferi, among others, have been shown to scavenge and manipulate host lipids for structural support, metabolism, replication, immune evasion, and disease severity. In thisReview, we will explore the importance of lipid hijacking for the growth and persistence of these microbes in both mammalian hosts and arthropod vectors.
Neurovascular coupling and bilateral connectivity during NREM and REM sleep
Authors: Turner Kevin L , Gheres Kyle W , Proctor Elizabeth A , Drew Patrick J
Published: October 2020

To understand how arousal state impacts cerebral hemodynamics and neurovascular coupling, we monitored neural activity, behavior, and hemodynamic signals in un-anesthetized, head-fixed mice. Mice frequently fell asleep during imaging, and these sleep events were interspersed with periods of wake. During both NREM and REM sleep, mice showed large increases in cerebral blood volume ([HbT]) and arteriole diameter relative to the awake state, two to five times larger than those evoked by sensory stimulation. During NREM, the amplitude of bilateral low-frequency oscillations in [HbT] increased markedly, and coherency between neural activity and hemodynamic signals was higher than the awake resting and REM states. Bilateral correlations in neural activity and [HbT] were highest during NREM, and lowest in the awake state. Hemodynamic signals in the cortex are strongly modulated by arousal state, and changes during sleep are substantially larger than sensory-evoked responses.
Comparative analysis of the metabolically active microbial communities in the rumen of dromedary camels under different feeding systems using total rRNA sequencing
Authors: Rabee Alaa Emara , Forster Robert , Elekwachi Chijioke , Sabra Ebrahim , Lamara Mebarek
Published: October 2020

Breakdown of plant biomass in rumen depends on interactions between bacteria, archaea, fungi, and protozoa; however, the majority of studies of the microbiome of ruminants, including the few studies of the rumen of camels, only studied one of these microbial groups. In this study, we applied total rRNA sequencing to identify active microbial communities in 22 solid and liquid rumen samples from 11 camels. These camels were reared at three stations that use different feeding systems: clover, hay and wheat straw (G1), fresh clover (G2), and wheat straw (G3). Bacteria dominated the libraries of sequence reads generated from all rumen samples, followed by protozoa, archaea, and fungi respectively. Firmicutes, Thermoplasmatales, Diplodinium, and Neocallimastix dominated bacterial, archaeal, protozoal and fungal communities, respectively in all samples. Libraries generated from camels reared at facility G2, where they were fed fresh clover, showed the highest alpha diversity. Principal co-ordinate analysis and linear discriminate analysis showed clusters associated with facility/feed and the relative abundance of microbes varied between liquid and solid fractions. This provides preliminary evidence that bacteria dominate the microbial communities of the camel rumen and these communities differ significantly between populations of domesticated camels.