bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence Of Local Taxes And Local Levies On The Regional Original Income Of Jakarta Special Capital Region DKI Jakarta
Authors: Asyah Harisandi Ashila , Akhfanabila Nadya , Windiana Kirani Rizky , Saleh Sadikin Dikdik , Idawati Wiwi
Published: September 2024

The covid-19 pandemic has caused a decline in growth in several sectors of the Indonesian economy. However, this does not affect DKI Jakarta's local revenue because based on data obtained by the Central Bureau of Statistics, it continues to increase every year even though the economy has slumped during the pandemic. This study aims to determine and analyse the effect of local taxes and levies on local revenue in DKI Jakarta Province for the period 2010-2022. The data used in this study are secondary data obtained through quantitative methods. The population and samples used are the realisation reports of the Regional Revenue and Expenditure Budget for the period 2010-2022. The analysis method used in this research is multiple linear regression analysis, including normality test, classical assumptions, and hypothesis testing. Partial research results show that local taxes have a positive effect on regional original income, while local levies has no significant effect on regional original income. Simultaneously, local tax and local levies have a positive influence on regional original income. A well-managed local tax will increase local revenue, which will logically increase regional original income. Meanwhile, regions that experience difficulties in managing and collecting retribution, including the lack of supporting infrastructure and inadequate human resources, cause the low contribution of retribution to regional original income.
The Art of Puppetery in Demak: History of Islamic Da'wah and Lakon Carangan Created by Sunan Kalijaga
Authors: Bagaskara Akbar , Rokhani Umilia , Jati Galih Pangestu
Published: September 2024

The purpose of this article is to analyze in general the phenomenon of puppetry in Demak Regency and its relation to Lakon Carangan created by Sunan Kalijaga as a medium for Islamic preaching. The research method used in this research is a qualitative type with a triangulation validation technique. The results of this research show that Sunan Kalijaga's Lakon Carangan is not just an ordinary story, but a story that is thick with Islamic elements that have been adjusted, as a means of moral and spiritual education that reinforces Javanese Islamic identity. Therefore, the art of puppetry in Demak Regency in the modern era can indirectly contribute to the preservation and sustainable development of Islam in the community
ANALYSIS OF THE MARKETING STRATEGY OF HAJJ AND UMRAH (SAHARA) SAVINGS IN AN EFFORT TO INCREASE THE NUMBER OF CUSTOMERS AT BMT NU EAST JAVA PUJER BONDOWOSO BRANCH
Authors: Nuruddin Ilham
Published: September 2024

Marketing is something that covers all systems that have a relationship with the aim of planning and determining prices to promoting and distributing products (goods or services) that can satisfy consumers. Hajj and Umrah savings products (SAHARA) are savings that use the Mudharabah Mutlaqah contract, namely: (a cooperation agreement between shahibul maal and mudharib). In this regard, this research was carried out with the aim of keeping consumers attracted to the products being released with several strategies that have been implemented. In accordance with the focus of the study, the data search process was carried out using a qualitative research type approach using the literature. This research uses descriptive qualitative research to describe the marketing strategy for Hajj and Umrah savings products (SAHARA) in increasing customer interest at BMT NU Pujer Branch. BMT NU Pujer Branch releases SAHARA products using the mudharabah mutlaqah contract system which aims to make customers' dreams come true. In marketing SAHARA BMT NU Pujer Branch products through direct marketing to the public and other strategies using browsers and advertising. The operational supporting factor for BMT NU Pujer Branch in the Hajj and Umrah Savings (SAHARA) product is the contribution made by MWCNU which is an external supporting factor, while the internal factors are low daily deposits, competitive profit sharing, managed using sharia principles using contracts. Mudlarabah Mutlaqah. Then the inhibiting factors are the lack of potential human resources with knowledge of sharia principles, the elderly factor towards customers, the community's economy declining, lots of competition, and the lack of public trust in NU, especially in the BMT NU Pujer Branch.
CHANGES IN THE CONSUMPTION BEHAVIOR OF NURUL JADID BOARDING SCHOOL STUDENTS IN DAILY PHYSICAL APPEARANCE (CASE STUDY OF NURUL JADID BOARDING SCHOOL FEMALE STUDENTS)
Authors: Varala Arrow , Maulidah Rifqoh
Published: September 2024

This study aims to examine and describe the factors causing the consumptive behavior of female students at Pondok Pesantren Nurul Jadid in shaping their daily physical appearance, as well as to understand and describe how these female students implement Islamic consumption concepts. This objective is important because, in essence, the pesantren teaches principles of simplicity and wise consumption management in accordance with Islamic teachings. However, there is a gap between the practice of Islamic consumption principles taught by the pesantren and the consumptive behavior of the female students, particularly in the consumption of products related to physical appearance such as clothing, accessories, and excessive makeup. The lack of knowledge among the students regarding Islamic consumption concepts creates a misalignment with the teachings of Islamic simplicity and the minimalist lifestyle that has been promoted. Through a qualitative approach with a case study of the female students at Pondok Pesantren Nurul Jadid, this research delves deeper into how the factors causing the consumptive behavior of these students shape their daily physical appearance and how the principles of Islamic consumption are applied by the students through the pesantren’s policies. Data were collected through in-depth interviews and observation to explore key factors such as cultural, social, personal, and psychological influences that affect their consumption behavior. The results of the study indicate that: 1) The daily physical appearance of the female students is strongly influenced by these factors, which in turn affects their consumption behavior. 2) Although some students have an understanding of the principles of Islamic consumption, the implementation of these principles in practice has not been fully maximized.
IMPLEMENTATION OF RISK MANAGEMENT OF THE MUDARABAH CONTRACT AT BMT UGT NUSANTARA BONDOWOSO KOTA BRANCH
Authors: Wahidah Sofiatul
Published: September 2024

This study aims to explain the implementation of risk management in the mudarabah contract at BMT UGT Nusantara Bondowoso Kota Branch, especially in financing the agricultural sector. Qualitative methods are used to collect data through interviews, observations, and documentation studies. The results of the study show that the financing risks at BMT include payment congestion due to crop failures and customer dishonesty in reporting business results. The risk management implemented involves risk identification using the 5C principle (Character, Capacity, Capital, Collateral, Condition), risk measurement based on collectibility categories, risk monitoring through direct supervision and evaluation of customer finances, as well as risk control through warning letters and collateral confiscation. Although risk management at BMT UGT Nusantara Bondowoso Kota Branch is quite good, there are still shortcomings in socialization to customers about the importance of risk management. Increased accuracy in analyzing financing and training to customers is expected to minimize the risk of problematic financing for mudarabah.
RESILIENCE OF THE DUAL ROLE OF HOUSEWIVES OF MSME ACTORS IN ISLAMIC ECONOMIC PERSPECTIVE (JAMU DAY SEE MSMES IN SUMBERANYAR VILLAGE)
Authors: Amah Sa'ifa' Al-Khairateel
Published: September 2024

Resilience of double role of housewife as a perpetrator of MSMEs in the Islamic Emergency Perspective, by referring to the theory of four resolience pages of Coulson. The main purpose of this study is to understand how housewives running micro, small, and medium enterprises (UMKM) overcome their double role challenges, as well as how the application of Islamic economic values can support their resilience. This research is important because it provides insight into the mechanism used by housewives to manage pressure from double responsibility, and how the values of Islamic economy can be an effective tool to strengthen their resilience. It is relevant to provide support to the UMKM actors in overcoming the economic and social difficulties faced. The method used is qualitative method with participant observation approach. The results showed that the housewife of MSME Jamu Day Se experienced four resolience stages. In the early stages, they feel overwhelmed by dual roasting pressure. The phase stage is characterized by changes in view of difficulties as a learning opportunity and seek support. At the recovery stage, they show progress through time management, training, and community empowerment. At the final stage, they experience rapid development with improved skills, business effectiveness, and psychological stability. In addition, the application of the values of Islamic economy, such as patience and tawakal, helps in the early stages; Responsibility and Istiqomah supports at the phase stage; Ikhtiar and Qana'ah play a role in recovery; And generosity and justice facilitate rapid development and positive contribution. These values reinforce the resilience by increasing the commitment, integrity, and social impacts of business.
EL RECICLAJE POSTDIGITAL EN LA OBRA DE ALMUDENA LOBERA, MARIO SANTAMARÍA Y ROMÁN TORRE
Authors: Goicoechea De Jorge María
Published: September 2024

Este artículo es fruto de la investigación en torno al reciclaje postdigital en el seno de las prácticas artísticas contemporáneas y tiene como objetivo destacar la aplicabilidad del término e identificar algunas de sus estrategias compositivas en relación con las obras de tres artistas contemporáneos del ámbito español (Almudena Lobera, Mario Santamaría, Román Torre). En un primer apartado, se abordarán las características del reciclaje postdigital en las prácticas artísticas contemporáneas, para después profundizar en la definición de las estrategias de la transcodificación, la reapropiación tecnológica y el hacktivismo artístico, y, por último, se analizarán algunas obras de estos tres artistas españoles que interpelan al público mediante la construcción de objetos, instalaciones y mecanismos que visibilizan y subvierten nuestros hábitos de consumo tecnológico. Observaremos cómo hacen uso de la interpenetración fluida de los diferentes espacios expositivos, tanto analógicos como digitales, así como de la exploración de sus intersticios, creando objetos híbridos (phygital) que entran en conflicto con las nociones tradicionales asociadas con cada medio y proponen al público nuevas interfaces que revelan aquello que hemos invisibilizado en nuestra hibridación con la máquina.
Huertas Morales, Antonio (dir.). Edad Media Contemporánea. Monografías Aula Medieval, 6, Valencia, Proyecto Parnaseo de la Universitat de València, 2017, 205 pp.1
Authors: Zubillaga Carina
Published: September 2024

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Huertas Morales, Antonio (dir.). Magia, brujería, Inquisición. Monografías Aula Medieval, 10, Valencia, Proyecto Parnaseo de la Universitat de València, 2019, 170 pp.1
Authors: Mármol Ávila Pedro
Published: September 2024

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A Idade Média e os Livros Didáticos: Um Neomedievalismo
Authors: Simões Rodrigo
Published: September 2024

Conceito de difícil definição, é uma característica do neomedievalismo devido à sua contínua mudança e desenvolvimento acerca da construção em torno do conceito de Idade Média. Entendendo      o material didático a respeito da Idade Média como tal, o livro didático é muitas vezes o primeiro e/ou único contato do brasileiro com livros, caracterizando-se como um instrumento crucial nas salas de aula, fato que torna urgente a constante melhoria deste material e, consequentemente, dos debates e críticas a respeito dessas obras. Dessa forma, a figura da Idade Média escolar analisada por intermédio da teoria do neomedievalismo é o objeto de análise deste artigo.
Sustainability reporting by public sector entities
Authors:   Kyiv National Economic University named after Vadym Hetman, Kindratska Liubomyra
Published: September 2024

Introduction. The importance of studying topical issues of non-financial information formation and preparation of non-financial reporting by public sector entities, in particular, sustainable development reporting, is obvious from the standpoint of increasing the role and importance of public sector institutions in the national economic system. Problem Statement. The issue of non-financial reporting in the context of the implementation of institutional measures related to the improvement of reporting on sustainable development in the context of crisis phenomena in the economy, increasing the role of accounting and the accounting profession in achieving the Sustainable Development Goals should be considered from the standpoint of studying the international experience of standardizing non-financial reporting and updating Sustainability Report. The study of the conceptual foundations of integrated reporting, methodology and organization of its analytical support on the basis of risk analysis related to the activities of public sector institutions is being updated. Given the de facto military actions, economic practice is undergoing a reset to survive in war conditions. The transition to sustainable development will be possible when the conditions for stabilizing the development of the domestic economy are established. The purpose is to analyse the existing approaches to the formation of non-financial information, to generalize the procedure for its reflection in the form of open data in reporting on sustainable development of public sector entities from the point of view of their recognition as subjects of high public interest. Methods. Logical and abstract, based on elements of the methods of theoretical generalisation, analysis, grouping, comparison; structural and institutional analysis with a description of options for identifying facts of violation of the integrity of the accounting and analytical system. Conclusions. The issue of the formation of non-financial information and the preparation of non-financial reporting based on it was investigated in terms of ​​considering the functions of regulatory bodies in the process of standardizing approaches to the formation of reporting on sustainable development, in particular by public sector entities through the identification of provisions that need to be taken into account in practical activities. The innovative role of the accountant in the preparation of non-financial reporting, the development of provisions for its audit, and in the processes of improving the accounting system related to the procedures for displaying reliable information in reporting on sustainable development has been proven.
Computation of Analog Theorem for Dirichlet Eta Function and Riemann Zeta Function
Authors: Annamalai Chinnaraji
Published: September 2024

This paper discusses how the Dirichlet eta function relates to the alternative harmonic series and the Riemann zeta function in more detail. In this article, theorems on series involving the alternative harmonic series, the Dirichlet eta function, and the Riemann zeta function are provided to improve the research techniques further in the area of analytic number theory.
Estágio em Psicologia Hospitalar: um relato de experiência
Authors: Paixão Herika Morais , Felício Luanna Lua Sousa
Published: September 2024

Este artigo relata a experiência de estágio de uma acadêmica de psicologia em um hospital geral da rede pública. As atividades de estágio configuram-se como um ponto crucial no processo de formação acadêmica. No contexto da psicologia hospitalar, proporciona condições para o desenvolvimento de habilidades práticas e uma compreensão mais aprofundada da realidade do trabalho do psicólogo na instituição hospitalar. Assim, o objetivo deste estudo é relatar algumas das experiências e contribuições provenientes do estágio não obrigatório em psicologia hospitalar e como isso implica na formação acadêmica dos graduandos em psicologia. Para isso, foi utilizado um estudo descritivo, do tipo relato de experiência, tendo como principais embasamentos teóricos a psicologia da saúde e a psicologia hospitalar. Em suma, evidenciou-se um impacto significativo e positivo na formação acadêmica decorrente das atividades de estágio. Além do aprimoramento nos conhecimentos sobre a prática em psicologia hospitalar, também pôde-se observar um desenvolvimento nas áreas da saúde e nos processos de organização e gestão do sistema único de saúde.
Efeito de fertilizante orgânico a base de leonardita sobre características produtivas do tomate
Authors: Silva Lucas Henrique Maldonado da , Ruzzon Felipe Beltrame
Published: September 2024

Por consequência do Brasil ser um país tropical, hortaliças como o tomate podem sofrer constante estresse vegetal devido à baixa amplitude térmica. Em vista disso, este trabalho tem como objetivo avaliar o efeito do fertilizante orgânico BlackGold® a base de leonardita na redução do estresse vegetal e sua influência para as características produtivas do tomate. Por conseguinte, realizou-se um experimento a campo utilizando a cultivar Ap-529®, em delineamento de blocos casuliazados, com cinco tratamentos e quatro blocos, de 18/10/22 a 21/01/23. Cada parcela foi composta por 30 plantas, divididas em 3 ruas, com espaçamento entre linhas de 1 metros, 0,25 m entre plantas e adubadas por igual utilizando sistema de fertirrigação. Os tratamentos foram divididos com suas respectivas dosagens de BlacKGold, sendo T1 – testemunha não recebendo o produto, T2 – 1 L.ha-1, T3 – 2 L.ha-1, T4 – 3 L.ha-1 e T5 – 4 L.ha-1. As colheitas ocorreram uma vez por semana do dia 20/12/22 a 21/01/23, os frutos maduros coletados foram submetidos a contagem e pesagem de imediato. Posteriormente sendo avaliado número de frutos (uni), peso colhido (kg), peso por fruto (g), produtividade (kg.plantas-1) e produtividade (kg.ha-1). Os resultados obtidos foram significativos, com a dose de 4 L.ha-1 apresentando o melhor resultado em todas variáveis analisadas.
Molecular docking and molecular dynamic simulation studies to identify potential terpenes against Internalin A protein of Listeria monocytogenes
Authors: K Deepasree , Venugopal Subhashree
Published: September 2024

Introduction

Ever since the outbreak of listeriosis and other related illnesses caused by the dreadful pathogen Listeria monocytogenes, the lives of immunocompromised individuals have been at risk.

Objectives and Methods

The main goal of this study is to comprehend the potential of terpenes, a major class of secondary metabolites in inhibiting one of the disease-causing protein Internalin A (InlA) of the pathogen via in silico approaches.

Results

The best binding affinity value of −9.5 kcal/mol was observed for Bipinnatin and Epispongiadiol according to the molecular docking studies. The compounds were further subjected to ADMET and biological activity estimation which confirmed their good pharmacokinetic properties and antibacterial activity.

Discussion

Molecular dynamic simulation for a timescale of 100 ns finally revealed Epispongiadiol to be a promising drug-like compound that could possibly pave the way to the treatment of this disease.
Successful citizen science tools to monitor animal populations require innovation and communication: SealSpotter as a case study
Authors: Puskic Peter S. , Holmberg Ross , McIntosh Rebecca R.
Published: September 2024

In rapidly changing ocean systems, there is a dual need to engage and educate community members and carry out rapid data acquisition. There is a body of evidence to support community or citizen science projects as successful vehicles for achieving these goals, with a particular need to increase global ocean literacy. The online SealSpotter program is a citizen science initiative aimed at monitoring trends in Australian fur seal (Arctocephalus pusillus doriferus) populations and connecting people to the marine environment. Here we present the findings of five years of monitoring of fur seals using drone surveys counted by citizen scientists via SealSpotter. Over five years, global participants from 23 countries were engaged in counting seals with a focus on the annual breeding season and pup abundance, with an average of 13,479 images and 171,137 seals counted per year. SealSpotter participants presented more conservative (lower) counts than expert counts, however both groups detected similar trends in abundance, emphasizing success of the project aims that included obtaining a precise index of pup abundance; ultimately a more achievable goal than accuracy due to the difficulties in measuring absolute abundance. We reflect on SealSpotter’s accomplishments and highlight the potential for marine citizen science programs as important tools for addressing global ocean literacy needs. The SealSpotter program contributes to our understanding of marine ecosystems through a simple but effective citizen science program.
Complete continuum of care for maternal health services and determinants among mothers who gave birth in the last year in Chiro City, Eastern Ethiopia: a community-based cross-sectional study (2024)
Authors: Ali Mohammed Ahmed , Geremew Habtamu , Abate Alegntaw , Bamlaku Golla Eyasu , Simegn Mulat Belay , Kumbi Hawi , Gichew Wondie Smegnew , Abdisa Samuel , Legasu Temesgen Dessalegne , Chekole Moges Sisay
Published: September 2024

Introduction

Ethiopia has one of the highest maternal death rates in the world, with 412 deaths for every 100,000 live births. A qualified healthcare professional's care during pregnancy, delivery, and the postnatal period is crucial to saving the lives of the mother and her child. The continuum of maternity health services in Ethiopia is still shallow, despite efforts to increase the use of these services. This study's goal was to evaluate the magnitude and factors influencing the entire continuum of care for maternal health services in Chiro City in 2024.

Methods

A community-based cross-sectional study was conducted from 15 January to 15 February 2024, among mothers who gave birth in the last year preceding the data collection period in Chiro City, Eastern Ethiopia. Data were collected through face-to-face interviews using a structured questionnaire. Multivariable logistic regression analysis was done to identify determinants of the complete continuum of care. A P-value of <0.05 was considered statistically significant in this study.

Result

The prevalence of a complete continuum of care among mothers who gave birth in the last year in Chiro District was 37.0%. Accordingly, women who received ANC were 5.24 times more likely to complete the maternal health services continuum of care compared with their counterparts (AOR: 5.24, 95% CI: 1.94, 14.15). Compared with their counterparts, women who received health education were 4.36 times more likely to complete the continuum of care (AOR: 4.36, 95% CI: 2.0, 9.51). Similarly, women who had planned pregnancies were 4.32 times more likely to complete the maternal health services continuum of care than those who had unplanned pregnancies (AOR: 4.32, 95% CI: 2.38, 7.85).

Conclusion and recommendation

The continuum of care was favorably correlated with ANC usage, health education, and planned pregnancies. To increase the coverage of the entire continuum of maternal care, it is desirable to develop programs that encourage women to initiate ANC contacts, provide health education, and promote planned pregnancies.
The nuclear GYF protein CD2BP2/U5–52K is required for T cell homeostasis
Authors: Bertazzon Miriam , Hurtado-Pico Almudena , Plaza-Sirvent Carlos , Schuster Marc , Preußner Marco , Kuropka Benno , Liu Fan , Kirsten Andor Zenon Amandus , Schmitt Xiao Jakob , König Benjamin , Álvaro-Benito Miguel , Abualrous Esam T. , Albert Gesa I. , Kliche Stefanie , Heyd Florian , Schmitz Ingo , Freund Christian
Published: September 2024

The question whether interference with the ubiquitous splicing machinery can lead to cell-type specific perturbation of cellular function is addressed here by T cell specific ablation of the general U5 snRNP assembly factor CD2BP2/U5–52K. This protein defines the family of nuclear GYF domain containing proteins that are ubiquitously expressed in eukaryotes with essential functions ascribed to early embryogenesis and organ function. Abrogating CD2BP2/U5–52K in T cells, allows us to delineate the consequences of splicing machinery interferences for T cell development and function. Increased T cell lymphopenia and T cell death are observed upon depletion of CD2BP2/U5–52K. A substantial increase in exon skipping coincides with the observed defect in the proliferation/differentiation balance in the absence of CD2BP2/U5–52K. Prominently, skipping of exon 7 in Mdm4 is observed, coinciding with upregulation of pro-apoptotic gene expression profiles upon CD2BP2/U5–52K depletion. Furthermore, we observe enhanced sensitivity of naïve T cells compared to memory T cells to changes in CD2BP2/U5–52K levels, indicating that depletion of this general splicing factor leads to modulation of T cell homeostasis. Given the recent structural characterization of the U5 snRNP and the crosslinking mass spectrometry data given here, design of inhibitors of the U5 snRNP conceivably offers new ways to manipulate T cell function in settings of disease.
An update on leprosy immunopathogenesis: systematic review
Authors: Silva Marcos Jessé Abrahão , Silva Caroliny Soares , Brasil Thiago Pinto , Alves Ana Karoliny , dos Santos Everaldina Cordeiro , Frota Cristiane Cunha , Lima Karla Valéria Batista , Lima Luana Nepomuceno Gondim Costa
Published: September 2024

Introduction

Leprosy is a chronic infectious condition and the main cause of neuropathy that occurs brought on by M. leprae. It is known that the biological characteristics of the human host, such as the immunological ones, have a higher influence on the pathology of this disease than the intrinsic mechanisms of the bacterium. The objective of this work was to review the scientific knowledge about the relationship between immunopathology and the severity of leprosy.

Methods

A systematic review following the PRISMA 2020 recommendations was conducted in the PUBMED, LILACS, SciELO and Science Direct databases using articles in English, Portuguese or Spanish between January 2011 and May 2022 with the descriptors “Leprosy/Immunology”, “Cytokines” and “Mycobacterium leprae”. A methodological quality assessment was carried out using the JBI checklists.

Results

A total of 49 articles were included. There is a relationship of greater severity of infection associated with lower release of MHC molecules in response to PGL-1 that inhibit the promotion of resolving T lymphocytes arising from dendritic cells (DCs) stimulation. In addition, the differentiation of macrophage phenotypes dependent on the activation of PRRs can define activation and the distinct type of T helper (Th) cells involved according to severity. Activated CD8+ T cells also have distinct types at the appropriate poles of the disease, and B cells show at the most severe pole of the LL, specific induction of IgA and more Treg-type CD8+ T cells that further contribute to T cell anergy.

Conclusion

Therefore, the adaptive immune system aggravates nerve damage and defines the type of leprosy, while the innate immune system is considerably more significant in the onset of nerve damage, symptomatic of the initial presentation of illness and in several critical immune responses, including inflammation and elimination of dead M. leprae.
Host factor RBMX2 promotes epithelial cell apoptosis by downregulating APAF-1’s Retention Intron after Mycobacterium bovis infection
Authors: Wang Chao , Jiang Yanzhu , Yang Zhiming , Xu Haojun , Khalid Abdul Karim , Iftakhar Tahira , Peng Yongchong , Lu Lu , Zhang Lei , Bermudez Luiz , Guo Aizhen , Chen Yingyu
Published: September 2024

The Mycobacterium tuberculosis variant bovis (M. bovis) is a highly pathogenic environmental microorganism that causes bovine tuberculosis (bTB), a significant zoonotic disease. Currently, “test and culling” is the primary measure for controlling bTB, but it has been proven to be inadequate in animals due to their high susceptibility to the pathogen. Selective breeding for increased host resistance to bTB to reduce its prevalence is feasible. In this study, we found a vital host-dependent factor, RBMX2, that can potentially promote M. bovis infection. By knocking RBMX2 out, we investigated its function during M. bovis infection. Through transcriptome sequencing and alternative splicing transcriptome sequencing, we concluded that after M. bovis infection, embryo bovine lung (EBL) cells were significantly enriched in RNA splicing associated with apoptosis compared with wild-type EBL cells. Through protein/molecular docking, molecular dynamics simulations, and real-time quantitative PCR, we demonstrated that RBMX2 promotes the apoptosis of epithelial cells by upregulating and binding to apoptotic peptidase activating factor 1 (APAF-1), resulting in the alternative splicing of APAF-1 as a retention intron. To our knowledge, this is the first report of M. bovis affecting host epithelial cell apoptosis by hijacking RBMX2 to promote the intron splicing of downstream APAF-1. These findings may represent a significant contribution to the development of novel TB prevention and control strategies.
Which neurodevelopmental processes continue in humans after birth?
Authors: Sorrells Shawn Fletcher
Published: September 2024

Once we are born, the number and location of nerve cells in most parts of the brain remain unchanged. These types of structural changes are therefore a significant form of flexibility for the neural circuits where they occur. In humans, the postnatal birth of neurons is limited; however, neurons do continue to migrate into some brain regions throughout infancy and even into adolescence. In human infants, multiple migratory pathways deliver interneurons to destinations across the frontal and temporal lobe cortex. Shorter-range migration of excitatory neurons also appears to continue during adolescence, particularly near the amygdala paralaminar nucleus, a region that follows a delayed trajectory of growth from infancy to adulthood. The significance of the timing for when different brain regions recruit new neurons through these methods is unknown; however, both processes of protracted migration and maturation are prominent in humans. Mechanisms like these that reconfigure neuronal circuits are a substrate for critical periods of plasticity and could contribute to distinctive circuit functionality in human brains.
The neural effects of acupuncture for stroke: a protocol for systematic review and meta-analysis based on fMRI
Authors: Cai Shaojie , Zhou Yihao , Yang Siyu , Liu Yichang , Han Yixiao , Wang Dongyan , Shi Shuai
Published: September 2024

Background

Stroke is one of the most common causes of death and is the main cause of persistent and acquired disability in adults worldwide. Acupuncture is recommended by the World Health Organization as an alternative and complementary strategy for stroke treatment. However, the mechanism of the neural effects of acupuncture on stroke is still lacking a uniform conclusion. The purpose of this study is to clarify the neural effects of acupuncture for stroke from the perspective of neuroimaging.

Methods

Seven databases, including PubMed, Embase, Cochrane Library, Chinese National Knowledge Infrastructure, VIP Database, Wan-Fang Data, and Chinese Biomedical Database, will be searched comprehensively according to the search strategy. All of them will be retrieved from the date of database establishment to December 31, 2023. All randomized controlled trials and observational studies will be considered for inclusion. The risk of bias will be assessed by the Cochrane Risk of Bias tool. Functional imaging of the whole brain in the resting functional state will be the primary outcome. A meta-analysis of primary outcome will be performed using Seed-based D Mapping with Permutation of Subject Images software. The data will be synthesized using a random effects model. Meta-analysis of other clinical data will be performed using RevMan 5.3 software. Publication bias will be assessed through funnel plots. Reports will adhere to the Preferred Reporting Items for Systematic Review and Meta-Analysis.

Conclusion

This study will aggregate the results of independent studies to provide overall evidence for the neural effects of acupuncture for stroke.

Clinical Trial Registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=500306, Identifier CRD42024500306.
Characteristics of ear fullness and synaptic loss in ear fullness revealed by SV2A positron emission tomographycortical
Authors: Zhou En , Xiao Xuping , Liu Bin , Tan Zhiqiang , Zhong JiaYu
Published: September 2024

Objective

Studies on feeling of ear fullness (FEF) related to sudden sensorineural hearing loss(SSNHL) are limited. The mechanisms of FEF are unclear. This study aimed to explore the characteristics and related brain activation of SSNHL with FEF.

Methods

A total of 269 SSNHL patients were prospectively observed and divided into two groups, with FEF and without FEF. Fifteen SSNHL patients with FEF and 20 healthy controls (HCs) were recruited and underwent 18F-SynVesT-1 static PET. Standardized uptake values ratios (SUVr) of 18F-SynVesT-1 were computed between regions of interest.

Results

The occurrence of FEF was not related to the audiogram type or severity of hearing loss. There was a positive correlation between the degree of FEF and the degree of hearing loss. Recovery from FEF was not related to the audiogram shape, the degree of hearing loss or recovery. Fifteen SSNHL patients with FEF had relatively low 18F-SynVesT-1 uptake in the right middle frontal gyrus, right inferior frontal gyrus, right middle temporal gyrus, bilateral parietal lobe sub-gyral and left medial frontal gyrus, as compared with HCs. There was no relatively high 18F-SynVesT-1 uptake in the cerebral cortex.

Conclusion

The occurrence and recovery of FEF in SSNHL patients are not related to the classification, degree and recovery of hearing loss. The 18F-SynVesT-1 uptake in the cerebral cortex of patients experiencing SSNHL and FEF has shown alterations. This indicates that FEF may be related to cortical reorganization after the sudden impairment of unilateral auditory input.
Progress and challenges in integrated traditional Chinese and western medicine in China from 2002 to 2021
Authors: Zhou Man-Ci , Fei Yu-Tong , Lai Xiao-Zhen , Lan Jian , Liu Bo , Wang Zhi-Wei , Fang Hai , Liu Jian-Ping , Rong Hong-Guo
Published: September 2024

Objective

The World Health Organization advocated for enhanced integration of traditional medicine and complementary medicine into national healthcare systems across all countries. This study aims to explore the progress and challenges in integrated traditional Chinese and western medicine (ITCWM) in China over 20 years using nationwide data.

Methods

This cross-sectional study examined various facets of ITCWM in China in terms of policies, resources, services, and funding. National policy documents from 2009 onwards were retrieved from official websites of the Chinese government. Data on ITCWM resources, services and subsidies from 2002 to 2021 were extracted from the China Statistical Yearbooks of Chinese Medicine and China Health Statistical Yearbooks. Research fund projects with the ITCWM discipline were collected from the database of National Natural Science Foundation of China. A mixed method of both quantitative and qualitative approaches was employed to present a comprehensive overview of the collected data.

Results

This study presented five key findings. First, despite the issuance of 17 national policies by the Chinese government since 2009 to promote the development of traditional Chinese medicine (TCM), only three of them were specifically tailored for ITCWM. Second, although the average annual growth rates of ITCWM institutions, beds, and practitioners reached 0.35%, 10.56%, and 10.88% from 2002 to 2021, with more equitable allocation of ITCWM resources, the overall proportion of ITCWM remained below 5% in 2021. Third, progress has been made in ITCWM practices, yet service efficiency requires further enhancement. In 2021, ITCWM hospitals accounted for 2% of outpatient and emergency visits and 1.57% of hospital admissions among all hospitals, and 9.82% of delivered services were preventive healthcare services. Fourth, ITCWM served a crucial role in primary healthcare services, but its service capabilities need improvement. From 2007 to 2021, the average growth rates of ITCWM clinics, outpatient departments, and practitioners in outpatient departments were 13.30%, 2.57%, and 12.14%, respectively, while the proportion within TCM hospitals dropped. Lastly, despite the Chinese government’s emphasis on financial investment and related project funding for ITCWM, it remained lower than that allocated to TCM and western medicine.

Conclusion

ITCWM played a pivotal role in China’s healthcare system to advance individuals’ health and well-being across the lifespan. In the future, China will provide further support to enhance ITCWM health resources and improve service capability, and the strategic integration of ITCWM into the broader healthcare system will play a crucial role in achieving universal health coverage and the Sustainable Development Goals.
Post-activation performance enhancement of flywheel and traditional squats on vertical jump under individualized recovery time
Authors: Sun Shaocheng , Yu Ying , Niu Yu , Ren Meiling , Wang Jiaoqin , Zhang Ming
Published: September 2024

Purpose

To explore the post-activation performance enhancement (PAPE) of flywheel and traditional squats on a series of vertical jumps, the loads of the two protocols were matched based on their linear velocities. In addition, we attempted to validate the effectiveness of determining individualized recovery time (IRT) between conditioning activities and explosive movements.

Methods

Sixteen trained players participated in three main experiments: first, one-repetition maximum (1RM) assessment and intensity matching test; second, the weighted jump squat (WJS) test at baseline and at 2, 4, 6, 8, and 10 min after flywheel and traditional protocols; and third, squat jump (SJ), countermovement jump (CMJ), and approach jump (AJ) tests incorporating IRT determined in the WJS sessions into both protocols. These protocols were standardized to 8 repetitions at 80% 1RM with equivalent concentric speed matched by a linear position transducer and conducted in a random order on separate days.

Results

In the WJS tests, both protocols exhibited significant increases on jump height (JH), peak force (PF), and peak power (PP) after 2 to 6 min (all p < 0.05), and the time courses of changes in performance were in a similar trend. In the SJ, CMJ, and AJ tests, both protocols demonstrated highly significant increases on JH, PP, and reactive strength index (RSI) after incorporating IRT (all p < 0.01), with all participants exhibiting diverse improvement above the baseline levels. The potentiation percentages of the flywheel protocol on JH, PP, and RSI were higher than those of the traditional protocol across four jumping types (JH: 5.35%–9.79% vs. 4.13%–8.46%; PP: 4.16%–6.13% vs. 3.23%–4.77%; and RSI: 7.27% vs. 7.04%).

Conclusion

High-intensity flywheel squats can produce jumping potentiation in neuromechanical factors comparable to, or even surpassing, those observed in traditional squats, potentially making them a more effective option for inducing PAPE. Additionally, incorporating IRT into potentiation protocols could further optimize the PAPE effects.
The relationship between social frailty and cognitive impairment among older adults: the role of various types of internet use
Authors: Choi Kyungwon , Ko Young
Published: September 2024

Introduction

This study aimed to explore the role of internet use in the relationship between social frailty and cognitive function among Korean older adults.

Methods

A nationally representative survey of community-dwelling older adults in Korea was used in the analysis (N = 8,639).

Results

All types of internet use were significantly associated with cognitive impairment and played a significant role in the relationship between social frailty and cognitive impairment. The advantage of internet use for information searching (OR 0.40, 95% CI 0.35–0.46) was the greatest for cognitive function, followed by internet use for instrumental use (OR 0.59, 95% CI 0.53–0.66). Internet use for entertainment exhibited the greatest influence in the relationship between social frailty and cognitive impairment, with interpersonal communication ranking second in significance. Internet use regulates the relationship between social frailty and cognitive impairment in older adults. The influences of internet use vary depending on the type of online activity and the levels of social frailty.

Discussion

This highlights the importance of considering various forms of internet use when developing non-pharmacological interventions to mitigate the impact of social frailty on cognitive decline.
Change of direction and linear speed relation to functional ability and joint mobility in Polish U19 volleyball and basketball 3 × 3 national teams
Authors: Czyznielewska Zuzanna , Gabrys Tomasz , Yagin Fatma Hilal , Cepicka Ladislav
Published: September 2024

The purpose of this study was to determine the extent of differences in the level of change in linear speed and velocity in the modified change of direction test (COD) and to determine the relationship between speed deficits resulting from changes of direction and functional performance between groups of Polish U19 Volleyball National Team and Polish Women's Basketball 3 × 3 National Team. A total of 23 athletes: 12 volleyball players (age: 18 ± 0 years; body height: 183 ± 7 cm; body weight: 70 ± 8 kg) and 11 basketball players (age: 26 ± 4 years; body height: 180 ± 6 cm; body weight: 73 ± 10 kg) participated in the study. Athletes were tested for the following measures: Functional Movement Screen test (FMS), dynamic balance test Y-Balance, joints range of motion measurements, maximal sprint test (14 m), modified COD test (14 m) and change of direction deficit (CODD). A value of p < 0.05 was considered statistically significant. There was no significant correlation between sprint and CODD results in basketball team. In volleyball team there was a positive and significant correlation between COD, sprint and CODD. There was a negative and significant correlation between Y-Balance scores and sprint test results in the basketball team. Basketball team had a positive significant correlation between hip rotations and COD results. There was a negative significant correlation between shoulder movements and COD and CODD results in volleyball team.
Evaluation of dietary supplementation of Ascophyllum nodosum and Lithothamnium calcareum as functional algae in F4+ Escherichia coli challenged piglets
Authors: Dell’Anno Matteo , Frazzini Sara , Reggi Serena , Ferri Irene , Scaglia Elena , Schiasselloni Linda , Inglesi Alessia , Riva Federica , Verdile Nicole , Pasquariello Rolando , Rossi Luciana
Published: September 2024

Introduction

Despite progress in reducing antimicrobial use in the veterinary field, it is crucial to find alternatives to preserve effectiveness and limit antimicrobial resistance. In pig farming, pathogenic strains of E. coli are the main cause of gastrointestinal disorders and antibiotic use. In this field, algae represent an innovation in animal nutrition that aligns with livestock sustainability principles and provide a high content of functional molecules.

Aim

The aim of this study was to evaluate the impact of an innovative dietary combination of Ascophyllum nodosum and Lithothamnium calcareum, on growth, duodenum gene expression, jejunum intestinal morphology, and serum oxidative status in F4+ Escherichia coli challenged piglets.

Materials and methods

Forty-eight weaned pigs, aged 28 ± 2 days, were divided into two groups (n = 24 pigs/group): the control group was fed a commercial diet (CTRL), while the seaweeds group was fed a commercial diet supplemented with 1.5% A. nodosum and 0.5% L. calcareum for 27 days (ALGAE). After 13 days, 50% of animals in each group were challenged with a single dose of 108 CFU/dose of E. coli F4+, resulting in two infected groups (CTRL+ and ALGAE+, n = 12 pigs/group). Growth performance was assessed by measuring the individual body weight. At day 27, from six animals/group duodenum and jejunum sections were sampled for gene expression analysis via qRT-PCR and histological evaluation.

Results and discussion

The results indicated a significantly higher body weight in the ALGAE+ group compared to CTRL+ after 7 days post-challenge (p < 0.0001). Jejunum morphology revealed lower villus height, villus width and villus height/crypt depth ratio in CTRL+ compared to ALGAE+ (p < 0.05) suggesting a protective effect of seaweeds on gut health.

Conclusion

In conclusion, algae mixture exerted a protective effect against intestinal damage from E. coli F4+ infection proposing A. nodosum and L. calcareum supplementation as interesting strategy to support animal growth, enhance health and reduce antibiotic treatments in weaned piglets.
Establishment and clinical application of a droplet digital PCR method for the detection of Edwardsiella tarda
Authors: Li Min , Li Xiaojun , Ye Yifei , Yin Jinfang , Mo Zuanlan , Xie Haiyan , Zhu Yanqiu , Zhong Liangning , Zhang Xianpeng , Bi Junlong
Published: September 2024

Edwardsiella tarda (E. tarda) can infect humans and a variety of animals, including fish, amphibians, reptiles, birds, and mammals. However, a more highly sensitive, specific, and repeatable test for its detection is lacking. The objective of this study was to develop a highly sensitive, specific, and repeatable droplet digital polymerase chain reaction (ddPCR)-based method for the quantitative detection of E. tarda. The gyrB gene was selected as the target gene, and primers and probe were designed and synthesized. Using E. tarda genomic DNA as templates, the reaction method was optimized to establish a linear relationship with real-time PCR detection methods. The sensitivity, specificity, and repeatability of the method were analyzed, and clinical samples were tested. When the primer and probe concentrations were 900 and 300 nM, respectively, and the annealing temperature was 57°C, the efficiency of the ddPCR amplification reaction was highest and the boundary between positive and negative droplet distribution was clearest. The sensitivity was high, with detection limit being as low as 0.56 copies·μL−1; additionally, and a good linear relationship (R2 = 0.9962) between ddPCR and real-time PCR detection, within the range of 1–25,000 copies·μL−1, was evident. The repeatability was good, with a detection coefficient of variation of 2.74%. There was no cross-reactivity with 15 other common pathogenic microorganisms in aquatic animals (Streptococcus agalactiae, Streptococcus iniae, Streptococcus suis type 2, Nocardia seriolae, Vibrio parahaemolyticus, Aeromonas sobria, red sea bream iridovirus, decapod iridescent virus 1, enterocytozoon hepatopenaei, carp edema virus, Koi herpesvirus, goldfish hematopoietic necrosis virus, tilapia lake virus, viral nervous necrosis virus, or grass carp reovirus) in positive samples. Among the 48 clinical samples, including Bahaba taipingensis and its live food fish, pond water samples, and routine monitoring samples (Koi), 21 were positive for E. tarda, consistent with the bacterial isolation and identification results. The E. tarda ddPCR detection method has high specificity, sensitivity, and repeatability, can more accurately quantify E. tarda, and provides a useful reference for research related to this bacterium.