bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular detection of feline calicivirus (FCV) in cats with oral lesions
Authors: Saltık Hasbi Sait , Erdağı Zehra
Published: June 2024

Feline Calicivirus (FCV) is a major cause of oral lesions in cats with respiratory tract disease (RTD). FCV is a single-stranded, positive-polarity RNA virus that encodes three open reading frames (ORFs). Active virus excretion occurs through the saliva of cats infected with FCV, which belongs to the Vesivirus genus of the Caliciviridae family. Oral mucosal lesions caused by infectious agents in RTD lead to significant impairment in the quality of life of cats. RTD, which also affects the oral mucosa, is a common problem in cats.. Ten cats of different ages, breeds, and genders with ocular lesions were used in this study. At the time of sample collection, the veterinarian performed general and oral examinations on each animal. On oral examination,varying degrees of gingivitis, stomatitis, and ulceration symptoms were noted. Samples were extracted using a commercial viral nucleic acid isolation kit. Three out of ten samples (30%) were found to be positive for FCV using RT-PCR. Our study contributed significantly to our understanding of the relationship between FCV and disease severity by effectively and quickly detecting it in cats with oral lesions that were naturally infected. In conclusion, the high sensitivity, specificity, and potential for field sample testing make RT-PCR a very important and inevitable method for research and clinical diagnosis related to FCV infection in cats with oral lesions.
Evaluasi Usability Pada Simulasi Virtual Reality Perawatan Luka
Authors: Khomariyah Aniek Nurul , Arif Yunifa Mifachul , Nugroho Fresy , Karami Ahmad Fahmi
Published: June 2024

Kemajuan inovasi virtual reality (VR) telah menjadi pusat utama dalam simulasi medis, khususnya dalam persiapan perawatan luka. Artikel ini menguraikan kemajuan terbaru dalam penggunaan VR untuk mensimulasikan langkah-langkah pembungkus luka, dengan tujuan meningkatkan keterampilan klinis dan kualitas hidup pasien. VR menawarkan manfaat seperti pengalaman belajar yang lebih mendalam, aksesibilitas yang lebih baik, pelatihan mandiri dengan panduan simulator, dan kemampuan evaluasi kinerja. Penelitian ini juga menyoroti potensi VR untuk meningkatkan pengetahuan teoritis dalam pendidikan keperawatan. Diharapkan dengan menggunakan aplikasi VR ini, peserta pelatihan dapat meningkatkan pengetahuannya dan mengamankan lingkungan. Para peserta pelatihan mengapresiasi pengalaman VR mereka, sesuai dengan hasilnya, dan mereka bersedia menyarankan rekan-rekannya untuk memanfaatkan aplikasi VR ini. Dua puluh lima peserta memiliki nilai SUS 72,8 atau nilai C. Berdasarkan data demografi, peserta laki-laki hanya mendapat nilai C dengan nilai 71,2 sedangkan peserta perempuan mendapat nilai C dengan nilai 72,8. Namun, penelitian ini memiliki keterbatasan, seperti ukuran sampel yang kecil dan skenario pelatihan yang terbatas. Hasilnya, penelitian ini menyarankan penelitian lebih lanjut dalam jangka panjang untuk menggeneralisasi temuan tentang dampak penggunaan VR dalam pelatihan perawatan luka.
La inevitable sombra
Authors: Posada Morales Juan Esteban
Published: June 2024

¿Cómo narrar la angustia de escribir, de vivir y de ser? La inevitable sombra es la búsqueda desesperada por responder tales preguntas incursionando en la mente de un escritor. La vida de un profesor universitario, colombiano e hijo de un desaparecido es el marco de esta novela que discurre, con un lenguaje singular, por indagaciones detectivescas, por profundas introspecciones psicológicas y por acerbas críticas sociológicas. Entre espacios de un barrio al que se lo carcome la idea de progreso, entre certidumbres que la amistad le brinda, entre aguardientes que lo protegen contra la realidad y entre ritmos lentos de la música de The Clash el protagonista cree contar la versión de su vida mientras se enfrenta con sus viejos traumas al buscar el paradero de su padre y encontrar su propia identidad.
Hybrid Model of Fuzzy Logic and Genetic Algorithm for Product Assembly Sequence Optimization
Authors: Deswinda Novi , Hadiguna Rika Ampuh , Sirivongpaisal Nikorn
Published: June 2024

The sequence of product assembly affects the efficiency and effectiveness of production because it reduces cycle time and production costs and reduces production errors. The application of artificial intelligence is growing to optimize the problem of assembly sequences of components or products, including genetic algorithms and fuzzy logic. These two models can complement each other to produce the best assembly sequence. This research consists of several stages: model formulation, model analysis, solution, and model verification and validation. A hybrid fuzzy and genetic algorithms model can optimize product assembly sequences more effectively and efficiently. Fuzzy logic can help determine the variables that must be optimized, while genetic algorithms can help find the optimal solution by combining these variables. Experiments using hybrid fuzzy logic and genetic algorithms to minimize assembly cycle time have resulted in product part assembly sequences that accommodate all geometric constraints, including assistive devices.
Makna Representasi Ekologis Dalam Kakawin Sangutangis
Authors: Savitri Putu Diah , Suarka I Nyoman , Erawati Ni Ketut Ratna
Published: June 2024

Representasi merupakan cara untuk mendeskripsikan sesuatu yang telah dilihat atau dialami seseorang. Representasi ekologis yang terdapat dalam Kakawin Sangutangis meliputi gambaran alam yang akan digambarkan melalui penggambaran alam itu sendiri secara langsung serta dibalut dengan gaya bahasa (dalam kakawin disebut alaṃkara). Namun, dalam penelitian ini akan diteliti mengenai makna representasi ekologis dalam Kakawin Sangutangis. Tujuan dari penelitian ini yakni untuk mengetahui makna yang tersimpan dibalik representasi ekologis yang ada dalam KST. Sehingga khalayak ramai dapat memahami makna yang ada dalam KST. Penelitian ini tergolong dalam penelitian kualitatif dengan pendekatan ekologi sastra yang dibantu dengan teori semiotika Michael Riffaterre. Sedangkan metode dan teknik penelitian yang akan digunakan dalam penelitian ini dibagi menjadi tiga bagian yaitu (1) metode dan teknik pengumpulan data yang menggunakan metode studi kepustakaan yang dibantu dengan teknik alih aksara, alih bahasa, dan teknik catat; (2) metode dan teknik analisis data yang menggunakan metode analisis isi (konten) yang dibantu dengan teknik deskriptif analitik; (3) metode dan teknik penyajian hasil analisis data yang menggunakan metode formal. Hasil dari penelitian ini yakni akan diketahui makna yang tersimpan dibalik representasi ekologis yang ada dalam KST. Makna-makna yang ditemukan meliputi makna pelestarian alam dan makna pengendalian diri. Temuan penelitian ini akan berguna untuk motivasi dalam kehidupan sehari-hari.
En el rap, 10; en el colegio, 0. Prácticas letradas vernáculas adolescentes, capital lingüístico y fracaso escolar
Authors: Vargas Franco Alfonso
Published: June 2024

Existen en Colombia y en Hispanoamérica creencias dominantes sobre los niños y jóvenes que experimentan dificultades con el discurso escolar y problemas de fracaso educativo. En general, estas apuntan a que el bajo rendimiento en el estudio y la reprobación sistemática de años lectivos son consecuencia de la desmotivación del estudiante, de su falta de compromiso con sus deberes escolares, de los conflictos familiares, de la pobreza, etc. Sin embargo, este discurso oculta la responsabilidad de la escuela y la libera de todo compromiso con los conflictos que padecen los estudiantes que provienen de las clases trabajadoras y de los grupos minoritarios de la sociedad. Llama la atención que los estudiantes que se encuentran en forma permanente en una situación de riesgo frente a la deserción escolar y el fracaso educativo desarrollan al margen de la institución escolar prácticas letradas vernáculas que les permiten resistir y, en el mejor de los casos, “negociar” con la lengua académica del colegio, y también construir su identidad en un escenario de lucha por la imagen que tienen de sí mismos, la cual se ve muy afectada por sus dificultades en la vida escolar. Este libro presenta los resultados de un estudio longitudinal, concebido como análisis de caso, sobre las luchas que libra un adolescente con el discurso educativo, la repetición de años escolares, el cambio de colegios, etc., y cómo encuentra en el rap, como práctica vernácula de literacidad, un significado a su vida personal, que le ha ayudado también a construir una relación menos traumática con la institución educativa y con el deseo de estudiar.
A Journey through Ages: Revisiting the Traumatized Female Characters in Glück's Poetry: A Study of Selected Poems
Authors: Abdullah Alaa' Ahmed
Published: June 2024

In the majority of her poems, Louise Glück fixes a great deal of her attention on traumatized female characters that are taken from different historical periods mainly by relying on Greco-Roman myths. Peculiar to her poetry, the common ground that linked, links, and will link female figures through time is the unchanging fact of being traumatized.  Glück's personal experience, which is deeply interwoven with these characters as she attempts to express not only her own trauma but the trauma of women in her time, will be explored and highlighted. In the selected poems, typically through two main recurring and excessively utilized mediums of expression namely religion and mythology, the presence of evidently traumatized female characters dominates the stage of her dramatized poetic setting.
The present study sheds light on PTSD theory pioneered by Cathy Caruth and will give a brief account of Louise Glück's life, her works, and style. It will analyze selected poems from Glück's poetry collection Poems 1962-2012 in the light of trauma theory. The showcased poems reveal how heavily Glück draws on mythology and religion as sources of her poetic output. These poems are Abishag, Persephone the Wonderer, Myth of Devotion, and Gretel in Darkness.
THE ATTITUDE OF BASIC SCHOOL EFL TEACHERS AND SUPERVISORS TOWARDS THE PROCESS OF SUPERVISION IN SULAIMANI CITY
Authors: Omer Chawan Taha , Abbas Abbas Mustafa
Published: June 2024

The current study is an extracted paper from an MA thesis that aimed to find out how Kurdish EFL instructors saw the effectiveness of their role as supervisors and how that perspective influenced their cognitive, behavioral, and affective scale of attitudes toward the decisions, made in the classroom concerning challenges which in this study, organically paves the way to examine the relationship between instructors' experiences as teachers and their views, feelings, and attitudes toward instructional leadership in Sulaimani City in Kurdistan region - Iraq. To conduct the study, a descriptive survey research design was employed and a concurrent type of mixed research method was used to enrich the data. The study was carried out in public basic schools in Sulaimani city using a simple random sampling technique. 365 randomly selected teachers filled out questionnaires and 8 supervisors were also included for the interview. Questionnaires and interviews were the main data gathering instrument for this study. Quantitative data collected through the questionnaire was analyzed by using mean scores, frequency, percentage, and independent sample t-test and SPSS version 20 was used for the analysis. Moreover, it aims to highlight the supervisors' perceptions and challenges they face in the implementation of this process, they are explored through a semi-structured interview. Findings of the study indicated that some supervisors display optimism regarding new supervision projects aimed at enhancing the educational system in Sulaimani city. Despite acknowledging certain drawbacks teachers exhibited a negative attitude towards basic school supervision in Sulaimani City and expressed dissatisfaction with how school-based instructional supervision was carried out, highlighting discrepancies in their approaches to fulfilling its objectives. Also, the results unveiled that teacher perceived their supervisors as inadequate in effectively carrying out school-based instruction supervision, leading to their dissatisfaction with the approaches employed in their schools. Furthermore, the study confirmed that various factors negatively impacted teachers' attitudes towards this process. These factors encompassed inappropriate supervisory approaches, supervisors' lack of essential skills and knowledge, diminished trust between teachers and supervisors, teachers' limited awareness of the importance and benefits of the process of supervision, inadequate feedback from supervisors, inadequate communications among teachers and supervisors, the influence of age and gender, as well as a lack of motivations and commitment among experienced teachers to engage collaboratively in implementation of basic school supervision.
Celebrated Fragmentation: A Postmodern Study of Harold Pinter’s “The Homecoming”
Authors: Abdullah Almaaroof Ansam Riyadh , Mehasin Zainab
Published: June 2024

Harold Pinter's "The Homecoming" is a play celebrated for its use of fragmentation. The play is known for its fragmented language, structure, and characters' fragmented identities and relationships. Through its fragmentation, the play creates a sense of disorientation and uncertainty, highlighting the characters' struggle to make sense of their experiences and relationships with each other. Harold Pinter is considered one of the most influential British playwrights of the 20th century. His work is often associated with the literary movement known as "Theatre of the Absurd," which emerged in the 1950s and 1960s. Pinter's work has been associated with a number of literary movements, including Overall, "The Homecoming" is a play celebrated for its use of fragmentation, which creates a sense of disorientation and uncertainty central to the play's themes and characters. The play emphasises the characters' struggle for identity and agency in an often oppressive and patriarchal world through its fragmented language and structure
Verbs of movements in Hebrew, Arabic and Aramaic languages, a comparative study in Semitic languages
Authors: Mahmood Safiya Shakir
Published: June 2024

This study is entitled “Hebrew, Arabic and Aramaic verbs of motion: a comparative study in Semitic languages (in syntax and semantics)” examines the definition, classification, grammatical and semantic roles of Hebrew, Arabic and Aramaic verbs of motion. The study also highlights the agreement and the differences between the three languages, especially in relation to their verbs. The study begins by presenting comprehensive definition of verbs of motion in Hebrew, Arabic and Aramaic it delves into the various aspects of these verbs, including their syntactic and semantic characteristics then it discusses the classification of these verbs, highlighting the various categories it can be grouped into based on their specific characteristics and usage patterns. in addition,  the study examines the grammatical and semantic roles of verbs of motion in those languages. It examines how these movements within sentences contribute to the overall meaning and structure of the language. The study analyzes the syntactic and semantic characteristics of these verbs, and sheds light on their role expressing movement, direction and other related concepts. In addition to discussing the points of similarity and agreement between the verbs of movement in Hebrew, Arabic and Aramaic, the study also highlights the differences between the three languages, especially in relation to their verbs of movement. It examines how these differences are expressed in terms of grammatical structure, semantic nuances, and usage patterns. Overall this study provides a comprehensive analysis of verbs of movement in Hebrew, Arabic and Aramaic. It offers a detailed examination of their definition, classification and grammatical and semantic roles in both language by highlighting the points of agreement and differences in relation to verbs of motion, the study contributes to a deeper understanding of the linguistic characteristics of Hebrew, Arabic and Aramaic.
Recurrent grammatical errors in student Russian-to-English consecutive interpreting
Authors: Lekomtseva Irina Alekseevna , Vyunova Ekaterina Kirillovna , Abdulmanova Adelya Khamitovna
Published: June 2024

Interpreting student errors have little been accorded full-scale treatment. The objective of the article is, then, to provide a classification of and explain some of the most recurrent grammatical errors made by students in Russian-to-English consecutive interpreting. Our findings suggest that all grammatical errors in student consecutive interpreting fall into four groups: errors in the use of articles; errors in the use of tenses; errors in the use of voice; and errors in the use of number. Among the key sources of grammatical errors in consecutive interpreting are inadequate linguistic competence and performance, i.e. tendency to transfer grammatical patterns specific to a native language into a foreign language, unpredictable syntax, quick speech motor actions; and psychological and cognitive factors, i.e. principles of analogy, severe time constraints. Error analysis in student consecutive interpreting can make a positive contribution into the practice of training interpreters as it reveals the problem triggers. Additionally, inquiries in error analysis can make valuable contributions in a linguistic typology of interpreting errors in typologically and structurally different languages, i.e. language-specific and language-pair specific errors.
Psychologically Informed Practice: 3 Low Back Pain Cases
Authors: Beneciuk Jason M. , Baker Sara , Tachibana Bryan , Vickers Ryan , Cristello Sara , Davis Anita L.
Published: June 2024

Background

Psychologically informed practice has become increasingly popular in physical therapy, but barriers to successful implementation in clinical practice still exist.

Case Presentation

Three patients with chronic low back pain (LBP) were managed with psychologically informed practice, using the STarT Back Screening Tool risk stratification, clinical practice guidelines, and patient-clinician communication to inform treatment decision making.

Outcome and Follow-Up

Clinically relevant improvements in pain and function were observed in all patients over an episode of care, with patient-reported outcome measure feedback being used to strengthen the therapeutic alliance and inform clinical decision making.

Discussion

Combining STarT Back Screening Tool risk stratification with clinical practice guideline recommendations can enhance clinical practice management of LBP. Psychologically informed practice has primarily focused on patients at high risk for persistent pain; however, it may be relevant for all patients with LBP, at varied intensities and dosage parameters. JOSPT Cases 2021;1(3):176–182. doi:10.2519/josptcases.2021.10008
The Importance of Early Recognition of Morel-Lavallée Knee Injury
Authors: Czujko Paul-Neil , Pierre-Glaude James , Gostic Cheri
Published: June 2024

A 26-year-old female recreational athlete described several shearing injuries to her left knee during a kickball tournament, after which she reported severe pain in her left knee and was unable to bear full weight on her left lower extremity. Five days following the injury, she was evaluated by an orthopaedist who, after a noncontributory radiograph of the left knee, recommended magnetic resonance imaging to rule out a meniscal tear. Magnetic resonance imaging revealed a medial Morel-Lavallée lesion. JOSPT Cases 2021;1(2):85–86. doi:10.2519/josptcases.2021.10061
A Parkour Athlete With Footballer’s Ankle: A Case Report
Authors: Gilsing Guus , de Kort Joris , van der Weegen Walter
Published: June 2024

Background

Anterior ankle impingement, or footballer’s ankle, is a common condition in which additional bone spurs or soft tissue is responsible for the pain. Conservative treatment is regularly unsuccessful, and large exostoses often require surgery.

Case Presentation

A 24-year-old parkour athlete was diagnosed with anterior ankle impingement, characterized by a sharp pain on the anterior part of his right ankle that made practicing parkour impossible. Imaging revealed large bony fragments at the superficial part of the anterior capsule (magnetic resonance imaging and X-ray). Initially, surgery was scheduled; however, due to a stress fracture of the navicular bone, treatment was changed to a conservative plan of physical therapy.

Outcome and Follow-Up

Eight weeks after the stress fracture, return-to-sport criteria were achieved: full ankle range of motion, absence of pain, optimal psychological readiness, and a less than 1% deficit on the single-leg triple hop test. At 8 weeks, the athlete was able to participate in a sponsor video without limitations.

Discussion

Large bony abnormalities in the anterior part of the ankle joint may be successfully treated with physical therapy. In this case, shared decision making was essential to create the tailor-made treatment plan. JOSPT Cases 2021;1(3):168–173. doi:10.2519/josptcases.2021.10100
Bilateral Quadriceps Tendon Rupture
Authors: Nelson Ryan P. , Dettmer Jonathan L. , Hoover Donald L.
Published: June 2024

A 36-year-old man presented via direct access to a physical therapist within 1 hour of injuring himself by jumping off a boat to a lakeside pier and landing in a full squat. Suspecting tendon rupture or avulsion fracture, the physical therapist immobilized both legs and referred the patient to an orthopaedic surgeon for radiographs, which showed bilateral quadriceps avulsion from the patella. JOSPT Cases 2021;1(4):273–274. doi:10.2519/josptcases.2021.10122
Mechanobiologically Oriented Rehabilitation of a Complex, Comminuted, Displaced Acetabular Fracture in a 70-Year-Old Medically Complicated Patient: A Case Report
Authors: White Mark
Published: June 2024

Background

Acetabular fractures account for an increasing number of bony injuries, particularly among the elderly. As many as 74% do not undergo surgical repair, some because of untenable medical risks and some because of a perceived lack of need, despite the risks for long-term sequelae. There is a gap in the literature regarding physical therapy treatment for the increasing number of patients with complex injuries of this type.

Case Presentation

A 70-year-old man slipped on ice and fell, sustaining a complex, comminuted, displaced acetabular fracture of the right hip. He was treated nonoperatively, utilizing mechanobiological principles to facilitate bony healing and repair and return to functional activities.

Outcome and Follow-Up

The total episode of care spanned 2.5 months. He was followed through the course of 2 weeks of inpatient rehabilitation and then 11 visits for home health. After the episode of care, his Patient-Specific Functional Scale score for ambulation was 7/10 and his global recovery rating was 80%. At 6.5 months, his Patient-Specific Functional Scale score was 9/10 and his global recovery rating was 95%.

Discussion

This case demonstrates the use of principles derived from the basic science and research literature about bone healing. It also suggests potential applicability to other types of fractures. JOSPT Cases 2021;1(3):185–196. doi:10.2519/josptcases.2021.10266
Aneurysmal Bone Cyst in a Female Military Trainee
Authors: Pulvermacher George Brett , Briggs Robert A. , Ortiz Teddy E.
Published: June 2024

An 18-year-old female trainee presented to primary care with 2 months of increasing bilateral hip pain. Due to the high risk and prevalence of bone stress injuries among military trainees, radiographs and magnetic resonance imaging were concurrently ordered to rule out femoral neck stress injury. She was referred to physical therapy per military trainee protocol. Imaging showed a large proximal right femur lesion, suggesting possible aneurysmal bone cyst. JOSPT Cases 2021;1(4):246–247. doi:10.2519/josptcases.2021.10269
Mirror Therapy as an Intervention Following Rotator Cuff Repair: A Case Report
Authors: Harder Brian , Walston Zach
Published: June 2024

Background

Mirror therapy has been studied primarily as an intervention following central sensitization and nociplastic conditions, such as complex regional pain syndrome, post-cerebrovascular accident pain, and phantom-limb pain.

Case Presentation

This case report is on a 48-year-old female musician treated with physical therapy following a rotator cuff repair with suprascapular nerve release.

Outcome and Follow-Up

This case report observed the pre-post measurements of range of motion following a mirror therapy protocol. The mean pre-post change in range of motion was 14.0°.

Discussion

Based on the results of the protocol, continued investigation of the application of mirror therapy in orthopaedic populations that have experienced prolonged immobilization may be warranted. JOSPT Cases 2021;1(3):143–147. doi:10.2519/josptcases.2021.10383
Brachial Plexopathy After Removal of a Shoulder Hemiarthroplasty Prosthesis: A Serial Case Study
Authors: Greathouse David G. , Painter Elizabeth E.
Published: June 2024

Background

Brachial plexopathy has been reported after shoulder arthroplasty and may be attributed to intraoperative tensioning, regional anesthesia, and other patient-specific factors.

Case Presentation

A 59-year-old woman presented for electrodiagnostic testing with severe right upper extremity weakness and sensory disturbance after removal of a right hemiarthroplasty humeral prosthesis 6 weeks prior. Physical examination revealed severe motor weakness and reduced sensation throughout the right upper extremity, with the greatest motor impairment in distal muscles.

Outcome and Follow-Up

Nerve conduction studies and electromyography (EMG) revealed a severe brachial plexopathy affecting axonal and myelin components of the lateral, medial, and posterior cords. Serial EMG and nerve conduction studies were performed 5 times over a 2-year period to inform prognosis, document neurological recovery, and guide rehabilitation.

Discussion

This case study demonstrates how the clinical examination, EMG, and nerve conduction studies guided diagnosis in a patient with a complex peripheral nerve lesion and details how EMG and nerve conduction studies may help predict neurological recovery. JOSPT Cases 2021;1(4):275–286. doi:10.2519/josptcases.2021.10783
Real-time display of intracranial subdural electrodes and the brain surface during an electrode implantation procedure using permeable film
Authors: Fujimoto So , Matsuo Takeshi , Nakata Yasuhiro , Shiojima Honoka
Published: June 2024

Background:

Subdural electrode (SDE) implantation is an important method of diagnosing epileptogenic lesions and mapping brain function, even with the current preference for stereoelectroencephalography. We developed a novel method to assess SDEs and the brain surface during the electrode implantation procedure using brain images printed onto permeable films and intraoperative fluoroscopy. This method can help verify the location of the electrode during surgery and improve the accuracy of SDE implantation.

Methods:

We performed preoperative imaging by magnetic resonance imaging and computed tomography. Subsequently, the images were edited and fused to visualize the gyrus and sulcus better. We printed the images on permeable films and superimposed them on the intraoperative fluoroscopy display. The intraoperative and postoperative coordinates of the electrodes were obtained after the implantation surgery, and the differences in the locations were calculated.

Results:

Permeable films were created for a total of eight patients with intractable epilepsy. The median difference of the electrodes between the intraoperative and postoperative images was 4.6 mm (Interquartile range 2.9–7.1). The locations of electrodes implanted outside the operation field were not significantly different from those implanted inside.

Conclusion:

Our new method may guide the implantation of SDEs into their planned location.
Acellular Flowable Dermal Matrix for Ostomy Reconstruction: A Safe and Effective Minimally Invasive Technique
Authors: Delgado-Miguel Carlos , Miguel-Ferrero Miriam , Díaz Mercedes , Camps Juan , López-Gutiérrez Juan
Published: June 2024

BACKGROUND: Acellular dermal matrices have long been used for complex abdominal wall closure and, more recently, for ostomy reconstruction. PURPOSE: To describe ostomy reconstruction with acellular flowable dermal matrix (AFDM) in a pediatric patient with a complex abdominal wall defect. CASE REPORT: A 14-year-old female who was diagnosed with unspecific hemorrhagic colitis at age 6 months and who underwent several abdominal surgeries (including total colectomy and terminal ileostomy) reported frequent, severe periostomy skin ulcerations secondary to improper fitting and ostomy bag leakage due to multiple periostomy skin retraction as a result of multiple abdominal scars. Under sedation, 2 small (5 mm) peristomal skin incisions were made, through which dissection and release of dermal scar tissue was performed. Afterwards, AFDM 40 mL was injected subcutaneously until a uniform, flat surface around the ostomy was achieved. The patient was discharged a few hours postoperatively, after verification of proper fit of the ostomy bag with no leakage. At 18-month follow-up, the patient was very satisfied with the result, with fewer ostomy bag changes and improved quality of life. CONCLUSION: This case report indicates that AFDM is a safe and effective minimally invasive technique for ostomy reconstruction, with minimal complications and satisfactory medium-term results.
Fetal Bovine Acellular Dermal Matrix in the Management of Chronic Nonhealing Lower Extremity Wounds
Authors: Benitez-Sanchez Susan , Rao Amit , Oropallo Alisha
Published: June 2024

BACKGROUND: The management of chronic nonhealing lower extremity wounds remains a problem that substantially affects patients and significantly burdens the health care system. Nonhealing wounds lead to increased hospitalization, decreased quality of life, minor and major amputations, and increased risk of mortality. Dermal matrices have advanced the science of wound healing. PURPOSE: To evaluate fetal bovine acellular dermal matrix (FBADM), an acellular dermal collagen repair scaffold derived from fetal bovine dermis, in the management of chronic nonhealing lower extremity wounds. METHODS: A single-center retrospective chart review was conducted to collect data on patients with chronic nonhealing lower extremity wounds treated with FBADM from January 2013 through December 2019. RESULTS: A total of 43 patients were enrolled, with a mean age of 68.5 years and a mean wound area of 27 cm2. Complete closure of the wound occurred in 53% of patients overall, with 28% of patients achieving healing within 12 weeks. CONCLUSION: Application of FBADM in the management of chronic nonhealing lower extremity wounds is safe, effective, and efficient.
PENGARUH PENAMBAHAN ION Ca2+ PADA PENGOLAHAN AIR GAMBUT MENGGUNAKAN KOAGULAN KITOSAN (THE EFFECT OF ADDITIONAL Ca2+ IONS ON PEAT WATER TREATMENT USING CHITOSAN COAGULANT)
Authors: Putri Tassya Amalia , Anis Anis Shofiyani , Titin Titin Anita Zaharah
Published: June 2024

Peat water generally has a brownish-red color and organic matter that can pollute the environment so it is not suitable for use. This study aims to determine the effectiveness of chitosan with the addition of salts containing Ca2+ ions, namely CaCO3 and CaCl2, on reducing peat water parameters. The work procedure includes the preparation of chitosan gel and the preparation of CaCO3 and CaCl2 solutions made with concentration variations of 100, 200, 300, 400, and 500 ppm. Determination of organic substance concentration was carried out by permanganometric titration method The effectiveness of increasing pH in the presence of CaCO3 and CaCl2 salts in peat water was 7. The highest turbidity reduction efficiency after coagulation in peat water was 41.98% with the addition of CaCO3 and 37.37%, with the addition of CaCl2 occurred at a concentration of 300 ppm. The highest efficiency of color reduction after coagulation of peat water was with the addition of 60.7% CaCO3, while with the addition of CaCl2 58% occurred with the addition of 200 ppm CaCl2. The efficiency of reducing organic matter in peat water after coagulation with the addition of CaCO3 was 48.35% at a concentration of 300 ppm while with the addition of CaCl2 it was 48.35%. The addition of Ca2+ ions from CaCO3 and CaCl2 salts is considered to increase the effectiveness of chitosan as a coagulant for treating peat water.
Membrane-immobilized transaminases for the synthesis of enantiopure amines
Authors: Meersseman Arango Hippolyte , Nguyen Xuan Dieu Linh , Luis Patricia , Leyssens Tom , Roura Padrosa David , Paradisi Francesca , Debecker Damien
Published: June 2024

For the manufacture of enantiopure amines, greener synthesis processes are needed. Transaminases (TAs) are able to produce chiral amines with excellent enantioselectivity and in mild conditions, and can be immobilized to target stability, recoverability, and reusability. In the perspective of process intensification, we propose to study TA immobilization onto polymeric membranes. Two main immobilization strategies were investigated, requiring prior membrane surface functionalization. On the one hand, a polyacrylonitrile (PAN) membrane surface was partially hydrolyzed and coated with polyethyleneimine (PEI) to electrostatically trap TAs. On the second hand, a polypropylene (PP) membrane was coated with polydopamine (PDA), which was subsequently modified with glycerol diglycidyl ether (GDE) in order to covalently graft TAs. The successful membranes functionalization was confirmed by surface characterization techniques (infrared spectroscopy, X-ray photoelectron spectroscopy, contact angle measurements, and scanning electron microscopy). Enzyme leaching was observed from the functionalized PAN membrane, highlighting the need to post-treat the reversibly immobilized TAs to improve their anchoring. The covalent coupling of TAs with PEI using glutaraldehyde (GA) was found highly effective to avoid leaching and to increase the enzyme loading, without affecting the specific activity of the biocatalyst. Similarly, the covalent grafting of TA onto functionalized PP membranes yielded very efficient biocatalysts (retaining 85 % specific activity with respect to soluble TA) displaying perfect recyclability throughout successive cycles. Immobilizing either the S-selective HeWT or the R-selective TsRTA resulted in robust heterogeneous biocatalysts with antagonist enantioselectivities. Thus, chiral amine synthesis can be performed effectively with biocatalytic membranes, which paves the way to intensified continuous flow synthesis processes.
Theory and Quantum Dynamics Simulations of Exciton-Polariton Motional Narrowing
Authors: Ying Wenxiang , Mondal Elious , Huo Pengfei
Published: June 2024

The motional narrowing effect has been extensively studied for cavity exciton-polariton systems in recent decades both experimentally and theoretically, which is featured by (1) the subaverage behavior, and (2) the asymmetric linewidths for the upper polariton (UP) and the lower polariton (LP). However, a minimal theoretical model that is clear and adequate to address all these effects, as well as the linewidth scaling relations remains missing. In this work, based on the single mode 1D Holstein-Tavis-Cummings (HTC) model, we studied the motional narrowing effect of the polariton linear absorption (LA) spectra via both semi-analytic derivations and numerically exact quantum dynamics simulations using the hierarchical equations of motion (HEOM) approach. The results reveal that under collective light-matter coupling between a cavity mode and $N$ molecules, the polariton linewidth scales as $1/\sqrt{N}$ under the slow limit, while scales as $1/N$ under the fast limit, due to the polaron decoupling effect. Further, by varying the detunings, the polariton linewidths exhibit significant motional narrowing, covering both characters mentioned above. Our analytic linewidth expressions agree well with the numerical exact simulations in all the parameter regimes we explored. These results indicate that the physics of motional narrowing is adequately accounted for by the single-mode 1D HTC model. We envision that both the numerical results and the analytic polariton linewidths expression presented in this work will offer great theoretical value for providing a better understanding of the exciton-polariton motional narrowing based on the HTC model.
Water-templated growth of interfacial superglue polymers for tunable thin films and in-situ fluid encapsulation
Authors: VSR Jampani , Skarabot Miha , Mur Urban , Baigl Damien , Jonas Ulrich , Lagerwall Jan , Ravnik Miha , Anyfantakis Manos
Published: June 2024

Thin polymer films are indispensable elements in numerous technologies ranging from liquid encapsulation to biotechnology to electronics. However, their production typically relies on wet chemistry involving organic solvents or chemical vapor deposition, necessitating elaborate equipment and often harsh conditions. Here, we demonstrate the eco-friendly, fast, and facile synthesis of water-templated interfacial polymers based on cyanoacrylates (superglues) that yield thin films with tailored properties. Specifically, by exposing a cationic surfactant-laden water surface to cyanoacrylate vapors, surfactant-modulated anionic polymerization produces a manipulable thin polymer film with a thickness growth rate of 8 nm/min. Furthermore, the shape and color of the film are precisely controlled by the polymerization kinetics, wetting conditions, and/or exposure to patterned light. Using various interfaces as templates for film growth, including the free surface of drops and soap bubbles, the developed method advantageously enables in-situ packaging of chemical and biological cargos in liquid phase as well as the encapsulation of gases within solidified bubbles. Simple, versatile, and biocompatible, this technology constitutes a potent platform for programmable coating and soft/smart encapsulation of fluids.
Probing the Ferredoxin:Hydrogenase Electron Transfer Complex by Infrared Difference Spectroscopy
Authors: ŞAHİN Selmihan , Brazard Johanna , Adachi Takuji B. M. , Milton Ross D. , Stripp Sven T.
Published: June 2024

Ferredoxins are small iron-sulfur proteins that engage in electron transfer (ET) with oxidoreductases across all domains of life. In bacteria, ferredoxins that contain two [4Fe-4S] clusters differ with respect to their electric midpoint potential: while “Alvin”-type ferredoxins show individual potentials between -500 and -650 mV vs. SHE, clostridial ferredoxins perform ET at indistinguishable potentials of approximately -400 mV vs. SHE. In this work, the electron transfer complex between clostridial ferredoxin CpFd and [FeFe]-hydrogenases from Clostridium pasteurianum (CpI) and green algae Chlamydomonas reinhardtii (CrHydA) was investigated spectroscopically. [FeFe]-hydrogenases are oxidoreductases that catalyze hydrogen turnover in bacteria and algae. Introducing the non-canonical amino acid para-cyanophenylalanine (pCNF) near to one of the iron-sulfur clusters of CpFd allowed for a quantification of electric field changes via the vibrational Stark effect (VSE) by Fourier-transform infrared (FTIR) spectroscopy. Upon reduction with H2 or auto-oxidation under N2, in situ FTIR difference spectroscopy reports on protein structural changes. Our data reveal that the affinity between ferredoxin and its redox partners is modulated by redox-dependent protein-protein interactions (PPIs). Prompted by these findings, we discuss whether clostridial ferredoxins might act as two-electron redox partners in contact with hydrogenase or other oxidoreductases.
Arenesulfenyl Fluorides: Synthesis, Structure, and Reactivity
Authors: Faialaga Nathan H. , Gephart Dana P. , Silva Breno D. , Liu Richard
Published: June 2024

Sulfenyl fluorides are organic compounds of sulfur in formal oxidation state +2 with the formula R–S–F. Although the chloride, bromide, and iodide analogues have been extensively described in the literature, organic sulfenyl fluorides remain essentially unstudied. These structures have been implicated as putative intermediates in established processes to access polyfluorinated sulfur species; however, definitive and direct evidence of their existence has not been achieved, nor has a systematic understanding of their reactivity. Here, we report the synthesis, isolation, and spectroscopic characterization of several arenesulfenyl fluorides, including structural analysis of 2,4-dinitrobenzenesulfenyl fluoride by single-crystal X-ray diffraction. The functional group undergoes direct, efficient, and highly regioselective anti-addition to alkenes and alkynes, as well as insertion by carbenes. The resulting α- or β-fluoro thioether adducts can be readily transformed into useful fluorinated motifs, for example by modification of the sulfur groups (to sulfonamides or sulfonyl fluorides), by sulfur elimination (to generate formal C–H fluorination products), or by Julia–Kocienski olefination (to form vinyl fluorides). Thus, we establish that sulfenyl fluorides are unexpectedly accessible and stable compounds. Further, they serve as versatile reagents for the production of fluorinated organic compounds.
Cu(II)/THPTA-Mediated Thiazolidine Deprotection for Living Phages and Cell Surfaces Labeling
Authors: Ma Chengyun , Liu Guoqing , Sun Jianan , Luo Disheng , Yin Juan , Yang Dechun , Pang Shuo , Hou Wei , Hemu Xinya , Ye Bang-Ce , Bi Xiaobao
Published: June 2024

Incorporating unnatural bioorthogonal groups into peptides and proteins offers an excellent opportunity to endow them with new properties in a precise and controlled manner. Among these, the α-oxo aldehyde group is particularly suitable for the post-functionalization of peptides and proteins due to its versatility and stability in aqueous buffers. However, the facile and site-specific incorporation of α-oxo aldehyde into proteins, especially in living systems, remains a long-lasting challenge. Here, we describe a novel Cu(II)/THPTA-Mediated Thiazolidine Deprotection (CUT-METHOD) strategy for post-installation of a highly-active α-oxo aldehyde moiety, which is released from a thiazolidine ring borne by a genetically encoded unnatural amino acid ThzK. This reaction is performed under physiological conditions, thereby enabling the chemoselective and site-specific modification of proteins via oxime ligation without compromising their integrity and function. To validate its versatility, we successfully performed site-specific incorporation of α-oxo aldehyde into recombinant proteins and those displayed on M13 filamentous bacteriophage particles and bacterial cell surfaces. In addition, by leveraging Spycatcher/Spytag chemistry and oxime ligation, the bacterial cells bearing aldehyde generated via the CUT-METHOD could be simultaneously decorated with two distinct functional molecules, providing a novel one-pot dual labeling platform for the construction of living bacterial cell-based cancer targeting systems. Put together, we have demonstrated that the CUT-METHOD strategy is a significant addition to the current bioorthogonal chemistry toolbox with broad applications anticipated in the near future.
GPT like transformer based conditional molecule generator and a high drug-likeness (QED) dataset generation
Authors: Xing Wen , Yang Juan
Published: June 2024

This study presents the development and evaluation of a novel GPT-like conditional molecule generator designed to optimize the synthesis of chemical compounds with desirable properties. The model incorporates six pivotal physicochemical properties as conditions: molecular weight, number of non-hydrogen atoms, ring count, hydrophobicity, quantitative estimation of drug-likeness (QED), and synthetic accessibility score (SAS). By integrating these specific attributes, the generator successfully produced a high-QED database, consisting of approximately 2 million molecules, all exhibiting a QED higher than 0.9. This achievement not only demonstrates the model's effectiveness in generating structurally diverse and potentially pharmacologically viable molecules but also underscores its utility in accelerating drug discovery processes.
Along-strike changes in ETS behavior near the slab edge of Southern Cascadia
Authors: Nuyen Carolyn , Schmidt David
Published: June 2024

Episodic tremor and slip (ETS) is well-documented along the entire length of the Cascadia subduction zone. We explore how the occurrence of ETS varies at the southernmost edge of the subduction zone, where geometric complexity and a slab window likely alter conditions along the plate interface. This work uses tremor and GNSS time series data to identify nineteen of the largest ETS events in southern Cascadia between 2016.5-2022 and document source properties for events approaching the slab edge. Distributed slip models for these events show that cumulative fault slip along the megathrust reaches a maximum near 40.5° N latitude and that large ETS events accommodate up to 85% of plate convergence at this location. However, ETS fault slip and tremor terminate near 40° N latitude, some 50 km before the southern lateral edge of the subducting plate. After considering a range of explanations, we propose that the complex geometry and progressive heating of the subducting plate modifies ETS behavior and does not allow seismic slip to occur along the plate interface in southernmost Cascadia below 35 km depth.
Impactos da pandemia na extensão universitária brasileira: as experiências e os desafios da Assessoria Jurídica Universitária Popular
Authors: Gomes de Abreu Gabriela , Orlando Borges de Oliveira Emanuelly , Fernandes Rocha Rogério
Published: June 2024

Neste artigo, registramos os principais impactos causados na atuação das Assessorias Jurídicas Universitárias Populares (AJUP) pela pandemia da COVID-19. As AJUP, enquanto extensões universitárias, tiveram seus projetos impactados pelas medidas de contenção da infecção. Utilizamos entrevistas semiestruturadas com membros de onze AJUP do Brasil, distribuídas entre as cinco regiões do país. Através do destaque das principais diferenças entre as formas de atuações das AJUP, antes e durante a pandemia, indicamos quais são os novos desafios enfrentados por elas em onze bases comuns de orientação-formação, propostas por Assis da Costa Oliveira. Apesar dos desafios, as novas formas de resistência e protagonismo das(os) ajupianas(os) indicam que o projeto de luta por direitos está vivo e pulsante.
Layer-finiteness of Some Groups
Authors:   Institute of Computational Modelling SB RAS, Senashov V. I.
Published: June 2024

Infinite groups with finiteness conditions for an infinite system of subgroups are studied. Groups with a condition: the normalizer of any non-trivial finite subgroup is a layer-finite group or the normalizer of any non-trivial finite subgroup has a layer-finite periodic part are studied for beginning in the locally finite class of group, then in the class of periodic groups of Shunkov and finally in the class of Shunkov groups which are contain a strongly embedded subgroup with an almost layer-finite periodic part. The group $G$ is called the Shunkov group if for any prime $p$ and for every finite subgroups $H$ from $G$ any two conjugate elements of order $p$ from the factor-group $N_G(H)/H$ generate a finite subgroup. Results for almost layer-finite groups and groups with almost layer-finite periodic part are transferred to layer-finite groups and groups with layer-finite periodic part. New characterizations of layer-finite groups and groups with layer-finite periodic part are obtained.
A Study of Multiserver Retrial Queues With Different Stages of Homogeneous Service
Authors: Renisagayaraj M  , Roja R  , Bhuvaneswari S  , Suganthi P  , Sujatha R
Published: June 2024

We discuss a queuing system with retrial of customers. Two models are discussed. First, we investigate single server queues in parallel, when the customer going to search and join the shorter of the two queues and in the second model we introduce the multiserver queue to multiserver retrial queue system. Multiserver provides different stages of homogeneous service in succession.