bluuebunny/crossref_metadata_2025 huggingface dataset:
Data from N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
Authors: Geroyska Sofia , Mejia Isabel , Chan Alfred A. , Navarrete Marian , Pandey Vijaya , Kharpatin Samuel , Noguti Juliana , Wang Feng , Srole Daniel , Chou Tsui-Fen , Wohlschlegel James , Nemeth Elizabeta , Damoiseaux Robert , Shackelford David B. , Lee Delphine J. , Díaz Begoña
Published: July 2024

Abstract
Myristoylation is a type of protein acylation by which the fatty acid myristate is added to the N-terminus of target proteins, a process mediated by N-myristoyltransferases (NMT). Myristoylation is emerging as a promising cancer therapeutic target; however, the molecular determinants of sensitivity to NMT inhibition or the mechanism by which it induces cancer cell death are not completely understood. We report that NMTs are a novel therapeutic target in lung carcinoma cells with LKB1 and/or KEAP1 mutations in a KRAS-mutant background. Inhibition of myristoylation decreases cell viability in vitro and tumor growth in vivo. Inhibition of myristoylation causes mitochondrial ferrous iron overload, oxidative stress, elevated protein poly (ADP)-ribosylation, and death by parthanatos. Furthermore, NMT inhibitors sensitized lung carcinoma cells to platinum-based chemotherapy. Unexpectedly, the mitochondrial transporter translocase of inner mitochondrial membrane 17 homolog A (TIM17A) is a critical target of myristoylation inhibitors in these cells. TIM17A silencing recapitulated the effects of NMT inhibition at inducing mitochondrial ferrous iron overload and parthanatos. Furthermore, sensitivity of lung carcinoma cells to myristoylation inhibition correlated with their dependency on TIM17A. This study reveals the unexpected connection between protein myristoylation, the mitochondrial import machinery, and iron homeostasis. It also uncovers myristoylation inhibitors as novel inducers of parthanatos in cancer, and the novel axis NMT-TIM17A as a potential therapeutic target in highly aggressive lung carcinomas.
Significance:
KRAS-mutant lung carcinomas with LKB1 and/or KEAP1 co-mutations have intrinsic therapeutic resistance. We show that these tumors are sensitive to NMT inhibitors, which slow tumor growth in vivo and sensitize cells to platinum-based chemotherapy in vitro. Inhibition of myristoylation causes death by parthanatos and thus has the potential to kill apoptosis and ferroptosis-resistant cancer cells. Our findings warrant investigation of NMT as a therapeutic target in highly aggressive lung carcinomas.
Data from E2F1-Associated Purine Synthesis Pathway Is a Major Component of the MET-DNA Damage Response Network
Authors: Poliaková Turan Michaela , Riedo Rahel , Medo Matúš , Pozzato Chiara , Friese-Hamim Manja , Koch Jonas P. , Coggins Si’Ana A. , Li Qun , Kim Baek , Albers Joachim , Aebersold Daniel M. , Zamboni Nicola , Zimmer Yitzhak , Medová Michaela
Published: July 2024

Abstract
Various lines of investigation support a signaling interphase shared by receptor tyrosine kinases and the DNA damage response. However, the underlying network nodes and their contribution to the maintenance of DNA integrity remain unknown. We explored MET-related metabolic pathways in which interruption compromises proper resolution of DNA damage. Discovery metabolomics combined with transcriptomics identified changes in pathways relevant to DNA repair following MET inhibition (METi). METi by tepotinib was associated with the formation of γH2AX foci and with significant alterations in major metabolic circuits such as glycolysis, gluconeogenesis, and purine, pyrimidine, amino acid, and lipid metabolism. 5′-Phosphoribosyl-N-formylglycinamide, a de novo purine synthesis pathway metabolite, was consistently decreased in in vitro and in vivo MET-dependent models, and METi-related depletion of dNTPs was observed. METi instigated the downregulation of critical purine synthesis enzymes including phosphoribosylglycinamide formyltransferase, which catalyzes 5′-phosphoribosyl-N-formylglycinamide synthesis. Genes encoding these enzymes are regulated through E2F1, whose levels decrease upon METi in MET-driven cells and xenografts. Transient E2F1 overexpression prevented dNTP depletion and the concomitant METi-associated DNA damage in MET-driven cells. We conclude that DNA damage following METi results from dNTP reduction via downregulation of E2F1 and a consequent decline of de novo purine synthesis.
Significance:
Maintenance of genome stability prevents disease and affiliates with growth factor receptor tyrosine kinases. We identified de novo purine synthesis as a pathway in which key enzymatic players are regulated through MET receptor and whose depletion via MET targeting explains MET inhibition-associated formation of DNA double-strand breaks. The mechanistic importance of MET inhibition-dependent E2F1 downregulation for interference with DNA integrity has translational implications for MET-targeting-based treatment of malignancies.
Inherited Telomere Biology Disorders: Pathophysiology, Clinical Presentation, Diagnostics, and Treatment
Authors: Rolles Benjamin , Tometten Mareike , Meyer Robert , Kirschner Martin , Beier Fabian , Brümmendorf Tim H.
Published: July 2024

Background: Telomeres are the end-capping structures of all eukaryotic chromosomes thereby protecting the genome from damage and degradation. During the aging process, telomeres shorten continuously with each cell division until critically short telomeres prevent further proliferation whereby cells undergo terminal differentiation, senescence, or apoptosis. Premature aging due to critically short telomere length (TL) can also result from pathogenic germline variants in the telomerase complex or related genes that typically counteract replicative telomere shortening in germline and certain somatic cell populations, e.g., hematopoetic stem cells. Inherited diseases that result in altered telomere maintenance are summarized under the term telomere biology disorder (TBD). Summary: Since TL both reflects but more importantly restricts the replicative capacity of various human tissues, a sufficient telomere reserve is particularly important in cells with high proliferative activity (e.g., hematopoiesis, immune cells, intestinal cells, liver, lung, and skin). Consequently, altered telomere maintenance as observed in TBDs typically results in premature replicative cellular exhaustion in the respective organ systems eventually leading to life-threatening complications such as bone marrow failure (BMF), pulmonary fibrosis, and liver cirrhosis. Key Messages: The recognition of a potential congenital origin in approximately 10% of adult patients with clinical BMF is of utmost importance for the proper diagnosis, appropriate patient and family counseling, to prevent the use of inefficient treatment and to avoid therapy-related toxicities including appropriate donor selection when patients have to undergo stem cell transplantation from related donors. This review summarizes the current state of knowledge about TBDs with particular focus on the clinical manifestation patterns in children (termed early onset TBD) compared to adults (late-onset TBD) including typical treatment- and disease course-related complications as well as their prognosis and adequate therapy. Thereby, it aims to raise awareness for a disease group that is currently still highly underdiagnosed particularly when it first manifests itself in adulthood.
Multi-Tissue Controls and Multiplex Immunocytochemistry in Pulmonary Cytology
Authors: Vuorisalo Antti , Haapaniemi Teppo , Kholová Ivana
Published: July 2024

Introduction: The World Health Organization 2021 lung cancer classification highlights the central role of immunohistochemistry (IHC) in diagnostic pathology. Despite traditional IHC being essential, its limitation to one marker per tissue section brings challenges, particularly when facing cytological limitedly sized samples. To overcome these challenges, multiplex immunocytochemistry (mICC) techniques offer the simultaneous detection of multiple markers from a single section. These advances complement the highly complex imaging techniques that enable additional analyses of cellular interactions. Methods: The present study outlines a comprehensive mICC methodology of an automated multiplex immunoperoxidase staining method and multiple tissue hybrid controls for ICC/mICC. Protocols are presented in detail and demonstrate a careful approach to optimizing various markers for diagnostic workup including immunotherapy. Conclusion: Multiplex IHC/ICC emerges as a transformative force in biomedical diagnostics and research. Beyond simultaneous marker detection, it unravels complexities within tissues – unveiling co-localization nuances, deciphering expression patterns, and enhancing understanding of cellular populations. As personalized treatments gain prominence, the study emphasizes the heightened importance of diagnostic tools and sample adequacy. The present methodological study, encapsulating an automated multiplex immunoperoxidase staining method, symbolizes a stride towards precision in pulmonary carcinoma diagnosis. Multi-tissue controls represent a key element in quality assurance in pathology laboratories.
Drug-Drug Interactions in Hospitalized Urological Patients: A Retrospective Cohort Study
Authors: Milovanovic Ivan R. , Pejcic Ana V.
Published: July 2024

Introduction: Exposure to potential drug-drug interactions (pDDIs) can be a notable source of avoidable drug-related harm that requires adequate management to prevent medical errors. We aimed to evaluate pDDIs and associated factors in hospitalized urological patients on admission, during hospitalization, and on discharge. Methods: A retrospective cohort study was conducted at the Clinic of Urology of the University Clinical Centre Kragujevac, Serbia. To detect pDDIs, we used Lexicomp, which categorizes pDDIs as follows: X (avoid combination), D (consider therapy modification), C (monitor therapy), B (no action needed), and A (no known interaction). Multiple linear regression analysis was used to identify factors associated with the number of pDDIs. Results: More than half of the 220 included patients had at least one pDDI on admission and discharge (57.3% and 63.6%, respectively), whereas 95.0% had at least one pDDI during hospitalization. The total number and number of X, D, C, and B categories of pDDIs were the highest during hospitalization and the lowest on admission. Duration of hospitalization, arrhythmias, dementia, renal failure, cancer, surgery during hospitalization, number of prescribed drugs, and various pharmacological drug classes were risk factors for a higher number of pDDIs, while age, ischemic heart disease, hypertension, and development of infection during hospitalization were protective factors in at least one of the stages. The impact of renal colic depended on the stage and category of pDDI. Conclusion: More than half of the urological patients were exposed to at least one pDDIs at all stages. Medical professionals should regularly screen for pDDIs, particularly in patients with risk factors.
Endobronchial Closure for Peripheral Pulmonary Air Leakage
Authors: Okado Shoji , Nakamura Shota , Sato Keiyu , Ueno Harushi , Ito Takayasu , Sato Kazuhide , Okachi Shotaro , Uehara Yohei , Matsumoto Misa , Shimazaki Natsumi , Miyagawa Katsuya , Chen-Yoshikawa Toyofumi Fengshi
Published: July 2024

Introduction: A minimally invasive alternative to surgery for treating pneumothorax has been developed, aiming to reduce risks while maintaining efficacy. This study conducted basic experiments using ex vivo and in vivo pig lung employing a super-thin catheter for treatment. This new device injects fibrin glue directly into the responsible lesion to close the air leak, which has two features: thin design and double-lumen. Methods: The experimental setup involved utilizing trachea and both lung specimens from pigs under positive pressure ventilation. To simulate pneumothorax, artificial fistulas were created on the lung surfaces. The super-thin catheter, guided through a bronchoscope near the fistula, was used to embolize the peripheral bronchus by injecting a fibrin preparation. Then, an air leak test was conducted afterward to assess the efficacy of the treatment. Additionally, a similar pneumothorax model was induced in alive pig under general anesthesia to evaluate its curability. Results: In the extracted pig lungs, embolization was performed in 21 cases, resulting in the cessation of air leaks in 19 cases, corresponding to a 90.5% cure rate. Notably, no major adverse events occurred with the treatment devices. Similarly, in living pigs, pneumothorax was successfully treated, with no recurrence observed up to the seventh postoperative day. Conclusion: The novel treatment device utilizing a super-thin catheter offers a minimally invasive and highly curative option for pneumothorax. These promising results suggest the potential for further development and human clinical trials, which could revolutionize the treatment of pneumothorax, reducing risks and improving outcomes.
A produção científica nas instituições da Rede Memorial de Pernambuco
Authors: Albuquerque Daniela Eugênia Moura de , Galindo Marcos
Published: July 2024

This article analyzes the scientific production on Pernambuco’s Memorial Network institutions in the field of Information Science. A descriptive bibliographic research using bibliometric and thematic analysis was conducted with 17 articles indexed on Information Science Database. A total of eight Memory Institutions were analyzed: Superintendência do Desenvolvimento do Nordeste, Laboratório Liber de Tecnologia do Conhecimento, Memorial da Justiça de Pernambuco, Fundação Joaquim Nabuco, Biblioteca Pública do Estado de Pernambuco, Paço do Frevo, Arquivo Público do Estado Jordão Emerenciano and Memorial Denis Bernardes. The study identified 32 authors, of which 50% are linked to the Federal University of Pernambuco. Of these, three are among the most productive and are professors at the university’s Department of Information Science. Ágora, Em Questão and Revista Brasileira de Biblioteconomia e Documentação were the most recurring journals, indicating a significant number of publications with good evaluation by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. The research brings contributions to Information Science by highlighting productions by these Memory Institutions and their developments for society and academia, such as sociocultural elements, the prioritization of studies focused on the state of origin, the integration of multiple competencies, interdisciplinarity, the democratization of knowledge and new knowledges produced by these spaces.
Association Between Cardiovascular Health and Subclinical Atherosclerosis Among Young Adults Using the American Heart Association's “Life's Essential 8” Metrics
Authors: Guo Fangqi , Chen Xinci , Howland Steve , Maldonado Luis E. , Powell Sydney , Gauderman W. James , McConnell Rob , Yan Mingzhu , Whitfield Lora , Li Yanjie , Bastain Theresa M. , Breton Carrie V. , Hodis Howard N. , Farzan Shohreh F.
Published: July 2024

Background

This study investigated the association of American Heart Association's cardiovascular health guidelines Life's Essential 8 (LE8) and Life's Simple 7 (LS7) with carotid artery outcomes among young adults.

Methods and Results

This cross‐sectional study included 240 young adults (age 24.2±1.6 years) who underwent a carotid ultrasound between 2018 and 2022. LE8 score was calculated from 4 health factors (body mass index, non–high‐density lipoprotein cholesterol, fasting glucose, and blood pressure), and 4 health behaviors (dietary intake, physical activity, tobacco use, and sleep). LS7 was calculated from 7 metrics (all LE8 metrics, except for sleep) with a simpler algorithm. Higher LE8 and LS7 scores both indicate better health and better adherence to American Heart Association guidelines. Carotid artery outcomes included carotid artery intima‐media thickness, arterial stiffness (eg, distensibility), and echogenicity determined by grayscale median of the intima media complex. Results of linear regression analyses, adjusting for age, sex, ethnicity, and parents' highest degree, indicated that a 1‐SD increase in LE8 score was associated with 12.14 μm lower carotid artery intima‐media thickness (95% CI, −20.93 to 3.35), 1.17 (10
−6
×m
2
/N) greater distensibility (95% CI, 0.09–2.24), suggesting less arterial stiffness, and 2.66 μm greater grayscale median of the intima media complex (95% CI, 0.58–4.75), suggesting less lipid deposition. Analyses using LS7 score demonstrated comparable findings. Health factor metrics demonstrated stronger association with carotid artery outcomes, as compared with behavior metrics.

Conclusions

Greater adherence to the American Heart Association's cardiovascular health guidelines is associated with lower risk for subclinical atherosclerosis in young adults. LE8 and LS7 demonstrated comparable associations with carotid artery outcomes.
Glymphatic System Impairment Contributes to the Formation of Brain Edema After Ischemic Stroke
Authors: Zhu Juan , Mo Jiaying , Liu Kewei , Chen Quanfeng , Li Zheqi , He Yihua , Chang Yuan , Lin Chuman , Yu Mingjia , Xu Yikai , Tan Xiangliang , Huang Kaibin , Pan Suyue
Published: July 2024

BACKGROUND:

Blood-brain barrier damage has traditionally been considered to determine the occurrence and development of poststroke brain edema, a devastating and life-threatening complication. However, no treatment strategy targeting blood-brain barrier damage has been proven clinically effective in ameliorating brain edema.

METHODS:

In mice with stroke models induced by transient middle cerebral artery occlusion (MCAO), the changes in glymphatic system (GS) function impairment were detected by ex vivo fluorescence imaging, 2-photon in vivo imaging, and magnetic resonance imaging within 1 week after MCAO, and the effects of GS impairment and recovery on the formation and resolution of brain edema were evaluated. In addition, in patients with ischemic stroke within 1 week after onset, changes in GS function and brain edema were also observed by magnetic resonance imaging.

RESULTS:

We found that the extravasation of protein-rich fluids into the brain was not temporally correlated with edema formation after MCAO in mice, as brain edema reabsorption preceded blood-brain barrier closure. Strikingly, the time course of edema progression matched well with the GS dysfunction after MCAO. Pharmacological enhancement of the GS function significantly alleviated brain edema developed on day 2 after MCAO, accompanied by less deposition of Aβ (amyloid-β) and better cognitive function. Conversely, functional suppression of the GS delayed the absorption of brain edema on day 7 after MCAO. Moreover, patients with ischemic stroke revealed a consistent trend of GS dysfunction after reperfusion as MCAO mice, which was correlated with the severity of brain edema and functional outcomes.

CONCLUSIONS:

GS is a key contributor to the formation of brain edema after ischemic stroke, and targeting the GS may be a promising strategy for treating brain edema in ischemic stroke.

REGISTRATION:

URL:
https://www.chictr.org.cn/showproj.html?proj=162857
; Unique identifier: NFEC-2019-189.
Can Language Models Handle Recursively Nested Grammatical Structures? A Case Study on Comparing Models and Humans
Authors: Lampinen Andrew
Published: July 2024

How should we compare the capabilities of language models (LMs) and humans? In this article, I draw inspiration from comparative psychology to highlight challenges in these comparisons. I focus on a case study: processing of recursively nested grammatical structures. Prior work suggests that LMs cannot process these structures as reliably as humans can. However, the humans were provided with instructions and substantial training, while the LMs were evaluated zero-shot. I therefore match the evaluation more closely. Providing large LMs with a simple prompt—with substantially less content than the human training—allows the LMs to consistently outperform the human results, even in more deeply nested conditions than were tested with humans. Furthermore, the effects of prompting are robust to the particular structures and vocabulary used in the prompt. Finally, reanalyzing the existing human data suggests that the humans may not perform above chance at the difficult structures initially. Thus, large LMs may indeed process recursively nested grammatical structures as reliably as humans, when evaluated comparably. This case study highlights how discrepancies in the evaluation methods can confound comparisons of language models and humans. I conclude by reflecting on the broader challenge of comparing human and model capabilities, and highlight an important difference between evaluating cognitive models and foundation models.
Usage-based Grammar Induction from Minimal Cognitive Principles
Authors: Jon-And Anna , Michaud Jérôme
Published: July 2024

This study explores the cognitive mechanisms underlying human language acquisition through grammar induction by a minimal cognitive architecture, with a short and flexible sequence memory as its most central feature. We use reinforcement learning for the task of identifying sentences in a stream of words from artificial languages. Results demonstrate the model’s ability to identify frequent and informative multi-word chunks, reproducing characteristics of natural language acquisition. The model successfully navigates varying degrees of linguistic complexity, exposing efficient adaptation to combinatorial challenges through the reuse of sequential patterns. The emergence of parsimonious tree structures suggests an optimization for the sentence identification task, balancing economy and information. The cognitive architecture reflects aspects of human memory systems and decision-making processes, enhancing its cognitive plausibility. While the model exhibits limitations in generalization and semantic representation, its minimalist nature offers insights into some fundamental mechanisms of language learning. Our study demonstrates the power of this simple architecture and stresses the importance of sequence memory in language learning. Since other animals do not seem to have faithful sequence memory, this may be a key to understanding why only humans have developed complex languages.
Exceptions, Instantiations, and Overgeneralization: Insights into How Language Models Process Generics
Authors: Allaway Emily , Bhagavatula Chandra , Hwang Jena D. , McKeown Kathleen , Leslie Sarah-Jane
Published: July 2024

Large language models (LLMs) have garnered a great deal of attention for their exceptional generative performance on commonsense and reasoning tasks. In this work, we investigate LLMs’ capabilities for generalization using a particularly challenging type of statement: generics. Generics express generalizations (e.g., birds can fly) but do so without explicit quantification. They are notable because they generalize over their instantiations (e.g., sparrows can fly) yet hold true even in the presence of exceptions (e.g., penguins do not). For humans, these generic generalizations play a fundamental role in cognition, concept acquisition, and intuitive reasoning. We investigate how LLMs respond to and reason about generics.

To this end, we first propose a framework grounded in pragmatics to automatically generate both exceptions and instantiations – collectively exemplars. We make use of focus—a pragmatic phenomenon that highlights meaning-bearing elements in a sentence—to capture the full range of interpretations of generics across different contexts of use. This allows us to derive precise logical definitions for exemplars and operationalize them to automatically generate exemplars from LLMs. Using our system, we generate a dataset of ∼370kexemplars across ∼17k generics and conduct a human validation of a sample of the generated data.

We use our final generated dataset to investigate how LLMs reason about generics. Humans have a documented tendency to conflate universally quantified statements (e.g., all birds can fly) with generics. Therefore, we probe whether LLMs exhibit similar overgeneralization behavior in terms of quantification and in property inheritance. We find that LLMs do show evidence of overgeneralization, although they sometimes struggle to reason about exceptions. Furthermore, we find that LLMs may exhibit similar non-logical behavior to humans when considering property inheritance from generics.
X-Band Radar and Surface-Based Observations of Cold-Season Precipitation in Western Colorado’s Complex Terrain
Authors: Heflin Stella , Abel Mimi , Biswas Sounak , Morales Annareli , Cifelli Rob , Sedlar Joseph , Feldman Daniel , Chandrasekar V. , Kennedy Patrick
Published: July 2024

Hydrologic processes associated with intermountain cold-season precipitation in the Upper Colorado River basin have important impacts on avalanche forecasting and water resource management. However, traditional weather radar networks struggle with observations in this complex terrain. Data collected during the Study of Precipitation, the Lower Atmosphere, and the Surface for Hydrometeorology (SPLASH) and its sister campaign, Surface Atmosphere Integrated Field Laboratory (SAIL) in the East River watershed of western Colorado, are used to examine a multistorm period from 23 December 2021 to 1 January 2022 that contributed 35% of the total winter precipitation in this watershed. Dual-polarization X-band radar and disdrometer measurements show ∼30-mm differences in precipitation amount at two sites in proximity over four distinct storm events within the period. Wind patterns, synoptic forcings, microphysical characteristics of precipitation, and surface meteorology are analyzed to explain the observed spatial variability of cold-season precipitation in complex mountainous terrain. Analysis shows that differences over time within this event are mainly accounted for by synoptic forcings, such as frontal passages; differences between sites are accounted for by the impact of variations in local wind patterns on precipitation microphysics. Patterns of surface precipitation intensity are compared and found to be correlated with X-band radar signatures; a relationship between a strong dendritic growth stage and intense low-density surface precipitation is reinforced by this study. This relationship demonstrates the importance of particle growth mechanisms on surface snowfall patterns in high-altitude complex terrain, underscoring the importance of realistic microphysical parameterizations.

Significance Statement

The amount and density of snowpack from western Colorado winter storms have significant impacts on water resources in the Upper Colorado River basin. Snowpack characteristics are affected by small-scale differences in how snow forms in the atmosphere. These differences are hard to study in the complex terrain of the Rockies, but data from the SPLASH and SAIL field campaigns allows us to investigate how snow crystal formation and mountain-driven wind patterns affect snow near the surface. Our study finds that snow crystal growth varies over small space and time scales and is likely controlled by the terrain beneath a given location and resultant local wind patterns. These results imply that predicting snowpack in the Rockies requires properly representing local wind patterns and crystal growth processes in models.
Conoce Tus Opciones: The Challenges of Communicating Rip Current Information in Spanish
Authors: Bernhardt Jase , Fallon Kathleen , Dusek Gregory
Published: July 2024

Rip currents are poorly understood by the public and thus a leading cause of weather-related fatalities in the United States. Individuals who speak languages other than English, such as Spanish-speaking communities, have been historically underserved by communication efforts for natural hazards and thus are at additional risk from rip currents. In response, the National Weather Service (NWS) has developed rip current outreach tools translated into Spanish in recent years, though their efficacy has not been systematically tested. We therefore surveyed members of the Spanish-speaking community in the New York City area to gain insights into how the NWS two-page rip current informational brochure is working and possible improvements to be made. The English version of the brochure was also tested, and the results from the two languages were compared. Quantitative survey results indicate that while both brochures are generally effective, several changes could be made to improve clarity, especially for Spanish speakers, who found more issues with the Spanish version. For example, the translation of the term rip current itself used by the NWS, “corrientes de resaca,” was determined to be confusing by some Spanish speakers, as were other pieces of text with complex scientific terms. Moreover, certain graphics contained in the brochure were difficult to understand for users in both languages. The results of this work can be used by the NWS and other agencies to improve their Spanish risk communication tools and can also be used as a guide when translating scientific information into other languages.

Significance Statement

Rip currents, narrow channels of fast-moving water that can pull swimmers away from the shore, are among the leading weather-related killers in the United States yet are poorly understood by the public. Recent efforts to reduce society’s vulnerability to rip currents include the translation of outreach materials from English to Spanish. This study evaluated a new National Weather Service Spanish rip current informational brochure and compared it to the English version. The results show that while both the English and Spanish brochures were generally effective at communicating risk, the Spanish version had more issues, primarily surrounding the translation of scientific terms and phrases. Findings from this research can help guide scientists in designing improved non-English severe weather outreach products.
From Foe to Friend: Exploring State-Led Destigmatization
Authors: Wang Milo Shaoqing , Steele Christopher W. J.
Published: July 2024

We seek to understand the distinctive process of state-led category destigmatization, extending an emergent stream of research on category destigmatization that has so far focused on the efforts of stigmatized members of categories. When a category conflicts with a prevailing ideology, internal actors may face such substantial barriers to destigmatization that the state—often motivated by the pragmatic benefits of that category’s success—must take a proactive role in the effort. Focusing on an extreme case, we explore the revival of the private business category in China through a longitudinal case study. We develop a grounded process model that highlights the interplay among the state, category members, and the public as a framework for understanding this type of destigmatization process. Our model also addresses dynamics that can emerge within the state when political power is divided between category proponents and opponents with competing ideological stances. Our study highlights the need for the state to balance destigmatization efforts with maintaining legitimacy, prompting iterative strategic adjustments based on local feedback, evolving public opinion, and intrastate competition between political factions. Our findings show that such adjustments may be needed even in authoritarian states, which are typically more coercive. In addition, we find that states can effectively use backstage strategies (e.g., regulatory leniency) and frontstage strategies (e.g., legislative change) in complementary ways to advance destigmatization while safeguarding the state’s legitimacy. Finally, we show that starting a category destigmatization effort by emphasizing the category’s pragmatic values (prior to advocating for moral reevaluation of the category) can mitigate ideological conflict and increase chances of successful destigmatization.
A Latent Class Analysis of Online Victim-Offender Overlap among Chinese Youth: Examining Overlap Risks across Online Deviance Types
Authors: Lin Kai , Zhou You , Xu Boyang , Chang Lennon Y. C.
Published: July 2024

This study aims to comprehensively test the applicability of lifestyle exposure theory (LET) against other criminogenic and victimogenic factors in predicting the differential risks of online offending-victimization overlap across multiple types of online deviance. Using self-reported survey data from 3,741 Chinese college students, the study performed Latent Class Analysis (LCA) and posterior multinomial logistic regression analysis. The LCA identified five latent classes of offending-victimization overlap, with only 6% of respondents reporting high overlap risk. Posterior multinomial logistic regression analysis showed that LET indicators and gender emerged as the most robust predictors of overlap risks compared to other theory-driven (e.g., control and routine activity theories) and sociodemographic factors. The current study accentuates the importance of methodological diversity in examining victim-offender overlap.
Alienated social workers in China’s communities: The impacts of role stress, professional efficacy, and state interventions
Authors: Wang Yean , Xie Zixiang , Zheng Guanghuai , Ma Mingzi
Published: July 2024

Chinese community-service social workers face work alienation due to role stress. This study highlights the impact of government resources on a role stress–professional efficacy–work alienation research model. Paradoxically, state support may escalate role stress, thus hindering professional development and causing work alienation in this state-dominated profession.
Teacher Feedback in Collegiate Instrumental Music Lessons
Authors: McPherson Gary E. , Blackwell Jennifer
Published: July 2024

Teacher feedback is an integral component of helping students understand their current level of achievement and what they can do to improve their performance. This study built on literature in educational psychology by adopting a framework developed by Hattie and Timperley to explore how different types of feedback are used in studio instrumental lessons. For this study, we investigated the use of feedback of highly experienced collegiate instrumental studio music teachers ( N = 6) and their students ( N = 18). Results indicated that around 83% of all teacher comments were classified as “feed-back” (i.e., reflections on what the student just played), with far fewer focused on “feed-forward” (16%) strategies for improving performance and “feed-up” (<1%) goal setting. Task-level comments on what the student had just done comprised 85% of the feedback, in contrast to process-level (9%) feedback that provides strategies for improving the next performance attempt and self-regulation comments (<1%) that cue the learner to monitor and control their own performance. Discussion of these results include comparisons between classroom and studio teaching practices and ways of refining instrumental teachers’ abilities to provide more targeted and effective feedback to their students.
Refinement of intranasal delivery in rats: A cadaveric study
Authors: Pereira Margarida N , Venâncio Carlos , Pinto Maria de Lurdes , Alves-Pimenta Sofia , Colaço Bruno
Published: July 2024

The intranasal route enables direct delivery of multiple substances from the nose to the brain, through olfactory and trigeminal pathways, bypassing the blood–brain barrier and avoiding systemic absorption. Despite the potential of this route, the various administration approaches make data reproducibility and interpretation challenging, emphasizing the necessity to establish a consistent methodology. Considering this, the aim of our study was to assess and compare the distribution of two dye volumes (30 µl and 50 µl) in the nasal cavity of rat cadavers. We employed three distinct methods of intranasal delivery: nose drops, by pipette tip, or cannula inserted into the nasal cavity. The results indicated that for both volumes, using the nose drops and the pipette tip methods, the dye dispersion occurred mainly in the vestibule, respiratory and olfactory regions, without reaching the olfactory bulbs. Using the cannula method, the deposition predominantly occurred in the respiratory and olfactory regions, with the dye reaching 66.7% and 100% of the olfactory bulbs, respectively, to low and high volume. Furthermore, the results demonstrated differences between the two volumes, in the pharynx, larynx, trachea, septal window, and incisive papilla, where an increased dye presence was observed with the 50 µl instillation across all three methods. According to our results, the intranasal delivery with a cannula was the most effective method for dye deposition in the olfactory region. However, further studies in live animals will be necessary to determine and refine the administration method that consistently allows specific deposition in the olfactory system.
A Comparative Analysis of Factors Influencing the Sustainability of the Abia State Health Insurance Agency: Insights From Rural-Urban Abia State
Authors: Ikechukwu Ukweh H. , Ofonime Ukweh N. , Joy Iya-Benson N. , Afiong Oku O. , Uchenna Ewelike , Chiesonu Iziga-Eruchalu G.
Published: July 2024

The goal of the Abia State Health Insurance Agency (ABSHIA) has been to increase coverage since its implementation. However, the sustainability of the scheme is crucial to continue providing affordable healthcare in the State. This study aimed to identify and compare factors that influence the sustainability of ABSHIA in rural-urban areas of Abia State. The study used a mixed-method cross-sectional design that involved collecting data through a questionnaire on enrollment, satisfaction, and willingness to renew membership. Key informant interviews and focus group discussions were also conducted to obtain qualitative data from healthcare providers and ward development committees. The collected data were analyzed using appropriate statistical tests. The results showed a higher enrollment in rural areas compared to urban areas, with no significant difference in satisfaction and willingness to renew membership between the 2 locations. The study also identified factors that positively influenced willingness to renew membership, but it was found that traveling a distance of 15 min or more decreased willingness to renew membership in urban Abia. Furthermore, poor health status was found to have a low influence on willingness to renew membership in rural Abia. Among other barriers to renewal, poor quality of care and, nonpayment of health workers’ capitation was identified as significant factors. It is crucial to prioritize the sustainability of ABSHIA to achieve the sustainable development goal of health for all in the State.
Efficacy of 0.1% Xylometazoline-Hydrochloride Nasal Decongestant Spray in Postoperative Sign/Symptom Relief Following Septoplasty: A Randomized Control Trial
Authors: Dawood Muhammad Hamza , Feroz Shanila , Sohail Sheza , Roshan Mavra , Perveen Haseefa , Nizam Tasmiha
Published: July 2024

Objective:This study evaluated the efficacy of 0.1% xylometazoline-hydrochloride nasal decongestant spray compared to 0.9% saline nasal spray in relieving post-septoplasty clinical nasal findings and symptoms. Methods: This triple-blinded randomized-clinical-trial was conducted in 2 tertiary-care hospitals in Karachi-Pakistan. A total of 120 septoplasty patients were recruited from June 20, 2022, to June 20, 2023. Randomly equally-assigned participants received either 0.9% isotonic-saline (control group) or 0.1% xylometazoline-hydrochloride (intervention group) nasal sprays. Participants were instructed to use nasal sprays for 1 week, twice daily, with a 12 hour interval between the 2 doses as a single spray per nostril. Follow-up assessments were conducted on days 3 and 7 after surgery. Postoperative symptoms, nasal-endoscopic findings, adverse-effects, and patient satisfaction were compared using Chi-square test and a P value of <.05 was considered significant. Results: Of 120 participants, 106 were analyzed with 53 participants in each group. By the end of third postoperative day, intervention group exhibited significantly lower rates of self-reported symptoms, including bleeding (7.5%:54.7%), nasal obstruction (3.8%:45.3%), headache (1.9%:30.2%), pain (3.8%:7.5%), as well as clinical nasal findings, including nasal edema (1.9%:58.5%), crusting (11.3%:58.5%), nasal discharge (9.4%:22.6%), and nonhealed scarring (18.9%:58.5%), compared to control group ( P value < .001). On the seventh day, intervention group exhibited absence of self-reported symptoms and clinical nasal findings, whereas control group still experienced moderate symptoms ( P value < .001). Intervention group had significantly higher patient satisfaction ( P value < .001). Conclusions: Xylometazoline spray is highly effective in relieving post-septoplasty clinical nasal findings and symptoms, without adverse effects, indicating potential for wider clinical utilization.

Trial Registration: UMIN Clinical-trial-registry: UMIN000052217. ( https://center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000059598 ).
The various facets of eliminationist politics: Conflict, nation-building, and forced migration
Authors: Garrity Meghan M. , Mylonas Harris
Published: July 2024

Eliminationist policies, as defined in this article, are deliberate actions by state or non-state actors that aim to destroy, remove, or erase certain groups on the basis of salient social identity characteristics. Conflict, nation-building, and forced migration scholars examine different facets of these policies, and related tactics, motivated by different research questions and methodological choices. This has resulted in academic silos, with varied conceptualizations, operationalizations, and scope conditions. These, in turn, limit theory-building and testing, introduce sources of potential bias, and undermine the generalizability of the findings. To address these shortcomings, we propose a broader field of ‘eliminationist politics’ encouraging cross-field dialogue. We propose that bringing these scholars into conversation will enhance theorizing, generate new research questions, and spur data collection efforts.
Adjusted Design Effect Model for Longitudinal Survey Data
Authors: Zhang Guoyi , Quazi Mohammed , Cheng Yang
Published: July 2024

In this research, we propose a longitudinal adjusted design effect model that integrates both the design effect and time effect, offering reliable estimators of variance. We investigate the asymptotic properties of certain types of estimators. Through simulation studies, we show the exceptional performance of the proposed method, demonstrating its superiority in producing accurate standard error estimators compared to existing approaches.
Diagnostic value of serum fibrin degradation complex DR-70 combined with conventional tumor biomarkers in colorectal cancer
Authors: Wang Xiaoxia , Wei Wei , Hua Nanni , Li Chunyan , Yu Lili
Published: July 2024

Objective

Most patients with colorectal cancer (CRC) show no early symptoms, and tumor markers have low sensitivity and specificity. We therefore investigated the ability of serum fibrin degradation complex DR-70 plus traditional tumor markers to diagnose CRC.

Methods

We retrospectively screened patients with CRC or non-malignant colorectal diseases, as well as healthy individuals, for inclusion in this study. The individuals’ clinical characteristics were recorded, and serum samples were collected. Expression levels of DR-70 and conventional tumor markers were measured by enzyme-linked immunosorbent assay and electrochemiluminescence.

Results

DR-70 levels differed significantly among patients with CRC, patients with benign colorectal diseases, and healthy individuals. Receiver operating characteristic curve analysis identified DR-70 as a conventional tumor marker with the highest sensitivity and the second-highest specificity after carcinoembryonic antigen.

Conclusions

This study identified DR-70 as a reliable marker for the detection, differentiation, and progression of CRC, with good sensitivity and specificity. DR-70 measurement could greatly improve the efficacy of CRC diagnosis when used together with other tumor markers.
Histopathologic evaluation system of African swine fever in wild boar infected with high (Arm07) and low virulence (Lv17/WB/Riel) isolates
Authors: Porras Néstor , Sánchez-Vizcaíno José M. , Barasona José Á. , Gómez-Buendía Alberto , Cadenas-Fernández Estefanía , Rodríguez-Bertos Antonio
Published: July 2024

To understand the clinicopathological forms of African swine fever (ASF) in wild boar, it is crucial to possess a basic knowledge of the biological characteristics of the currently circulating ASF virus isolates. The aim of this work is to establish an accurate and comprehensive histopathologic grading system to standardize the assessment of the ASF lesions in wild boar. The study evaluated the differences between animals infected with a high virulence genotype II isolate (Arm07) (HVI) through intramuscular (IM) (n = 6) and contact-infected (n = 12) routes, alongside those orally infected with a low virulence isolate (Lv17/WB/Riel) (LVI) (n = 6). The assessment included clinical (CS), macroscopic (MS), and histopathologic (HS) scores, as well as viral loads in blood and tissues by real-time quantitative polymerase chain reaction (qPCR). Tissues examined included skin, lymph nodes, bone marrow, palatine tonsil, lungs, spleen, liver, kidneys, thymus, heart, adrenal glands, pancreas, urinary bladder, brain, and gastrointestinal and reproductive tracts. The HVI group exhibited a 100% mortality rate with elevated CS, MS, and HS values. Animals infected by contact (CS = 12; MS = 58.5; HS = 112) and those intramuscularly infected (CS = 14.8; MS = 47; HS = 104) demonstrated similar values, indicating that the route of infection does not decisively influence the severity of clinical and pathological signs. The LVI group showed a 0% mortality rate, an inconspicuous clinical form, minimal lesions (CS = 0; MS = 12; HS = 29), and a lower viral load. Histopathologic evaluation has proven valuable in advancing our comprehension of ASF pathogenesis in wild boar and paves the groundwork for further research investigating protective mechanisms in vaccinated animals.
A Case Study of Ridership and Equity Implications of All-Day Massachusetts Bay Transportation Authority Commuter Rail Service
Authors: Polzin Samuel S. , Willis-Jackson Megan , White Phil , Meakin Karl , Koch Martha
Published: July 2024

As the COVID-19 pandemic emerged from the acute phase and vaccines became widely available in 2021, transit agencies like the Massachusetts Bay Transportation Authority (MBTA) faced a daunting challenge of drawing riders back into their systems. Despite systemwide staffing shortages, service disruptions, and ridership patterns that have yet to return to 2019 levels, ridership on MBTA’s Commuter Rail has consistently outperformed the agency’s other services in the current COVID recovery era. In April 2021, as part of a multiyear vision to overhaul the system, MBTA switched from a schedule focused on serving traditional peak-period commuters to providing steady, all-day service modeled on more legible “clockface” departures. This study used regression analysis to show this change was consistent with the Commuter Rail outperforming other modes in ridership recovery, generating over 7,000 average daily weekday boardings and over 9,000 average weekend boardings. Latent class analysis demonstrated that this schedule shift primarily benefited riders traveling for a variety of purposes, rather than just traditional office work. This group, which we referred to as general riders, was more likely to be low-income (household income below $75,000), young (25 or younger), and Hispanic. This research demonstrated the ability of all-day service on Commuter Rail to serve MBTA’s key aims of increasing ridership and providing more equitable, accessible service.