bluuebunny/crossref_metadata_2025 huggingface dataset:
Drug Repurposing in Cancer Therapy: Influence of Patient’s Genetic Background in Breast Cancer Treatment
Authors: Rodrigues Rafaela , Duarte Diana , Vale Nuno
Published: April 2022

Cancer is among the leading causes of death worldwide and it is estimated that in 2040 more than 29 million people will be diagnosed with some type of cancer. The most prevalent type of cancer in women, worldwide, is breast cancer, a type of cancer associated with a huge death rate. This high mortality is mainly a consequence of the development of drug resistance, which is one of the major challenges to overcome in breast cancer treatment. As a result, research has been focused on finding novel therapeutical weapons, specifically ones that allow for a personalized treatment, based on patients’ characteristics. Although the scientific community has been concerned about guaranteeing the quality of life of cancer patients, researchers are also aware of the increasing costs related to cancer treatment, and efforts have been made to find alternatives to the development of new drugs. The development of new drugs presents some disadvantages as it is a multistep process that is time- and money-consuming, involving clinical trials that commonly fail in the initial phases. A strategy to overcome these disadvantages is drug repurposing. In this review, we focused on describing potential repurposed drugs in the therapy of breast cancer, considering their pharmacogenomic profile, to assess the relationship between patients’ genetic variations and their response to a certain therapy. This review supports the need for the development of further fundamental studies in this area, in order to investigate and expand the knowledge of the currently used and novel potential drugs to treat breast cancer. Future clinical trials should focus on developing strategies to group cancer patients according to their clinical and biological similarities and to discover new potential targets, to enable cancer therapy to be more effective and personalized.
Mouse Mutants of Gpr37 and Gpr37l1 Receptor Genes: Disease Modeling Applications
Authors: Massimi Marzia , Di Pietro Chiara , La Sala Gina , Matteoni Rafaele
Published: April 2022

The vertebrate G protein–coupled receptor 37 and G protein–coupled receptor 37-like 1 (GPR37 and GPR37L1) proteins have amino acid sequence homology to endothelin and bombesin-specific receptors. The prosaposin glycoprotein, its derived peptides, and analogues have been reported to interact with and activate both putative receptors. The GPR37 and GPR37L1 genes are highly expressed in human and rodent brains. GPR37 transcripts are most abundant in oligodendrocytes and in the neurons of the substantia nigra and hippocampus, while the GPR37L1 gene is markedly expressed in cerebellar Bergmann glia astrocytes. The human GPR37 protein is a substrate of parkin, and its insoluble form accumulates in brain samples from patients of inherited juvenile Parkinson’s disease. Several Gpr37 and Gpr37l1 mouse mutant strains have been produced and applied to extensive in vivo and ex vivo analyses of respective receptor functions and involvement in brain and other organ pathologies. The genotypic and phenotypic characteristics of the different mouse strains so far published are reported and discussed, and their current and proposed applications to human disease modeling are highlighted.
Identification and Characterization of the MIKC-Type MADS-Box Gene Family in Brassica napus and Its Role in Floral Transition
Authors: Zhou Enqiang , Zhang Yin , Wang Huadong , Jia Zhibo , Wang Xuejun , Wen Jing , Shen Jinxiong , Fu Tingdong , Yi Bin
Published: April 2022

Increasing rapeseed yield has always been a primary goal of rapeseed research and breeding. However, flowering time is a prerequisite for stable rapeseed yield and determines its adaptability to ecological regions. MIKC-type MADS-box (MICK) genes are a class of transcription factors that are involved in various physiological and developmental processes in plants. To understand their role in floral transition-related pathways, a genome-wide screening was conducted with Brassica napus (B. napus), which revealed 172 members. Using previous data from a genome-wide association analysis of flowering traits, BnaSVP and BnaSEP1 were identified as candidate flowering genes. Therefore, we used the CRISPR/Cas9 system to verify the function of BnaSVP and BnaSEP1 in B. napus. T0 plants were edited efficiently at the BnaSVP and BnaSEP1 target sites to generate homozygous and heterozygous mutants with most mutations stably inherited by the next generation. Notably, the mutant only showed the early flowering phenotype when all homologous copies of BnaSVP were edited, indicating functional redundancy between homologous copies. However, no changes in flowering were observed in the BnaSEP1 mutant. Quantitative analysis of the pathway-related genes in the BnaSVP mutant revealed the upregulation of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FLOWERING LOCUS T (FT) genes, which promoted early flowering in the mutant. In summary, our study created early flowering mutants, which provided valuable resources for early maturing breeding, and provided a new method for improving polyploid crops.
Frequent Spindle Assembly Errors Require Structural Rearrangement to Complete Meiosis in Zea mays
Authors: Weiss Jodi D. , McVey Shelby L. , Stinebaugh Sarah E. , Sullivan Caroline F. , Dawe R. Kelly , Nannas Natalie J.
Published: April 2022

The success of an organism is contingent upon its ability to faithfully pass on its genetic material. In the meiosis of many species, the process of chromosome segregation requires that bipolar spindles be formed without the aid of dedicated microtubule organizing centers, such as centrosomes. Here, we describe detailed analyses of acentrosomal spindle assembly and disassembly in time-lapse images, from live meiotic cells of Zea mays. Microtubules organized on the nuclear envelope with a perinuclear ring structure until nuclear envelope breakdown, at which point microtubules began bundling into a bipolar form. However, the process and timing of spindle assembly was highly variable, with frequent assembly errors in both meiosis I and II. Approximately 61% of cells formed incorrect spindle morphologies, with the most prevalent being tripolar spindles. The erroneous spindles were actively rearranged to bipolar through a coalescence of poles before proceeding to anaphase. Spindle disassembly occurred as a two-state process with a slow depolymerization, followed by a quick collapse. The results demonstrate that maize meiosis I and II spindle assembly is remarkably fluid in the early assembly stages, but otherwise proceeds through a predictable series of events.
Activity of Silver Nanoparticles against Staphylococcus spp.
Authors: Swolana Denis , Wojtyczka Robert D.
Published: April 2022

Staphylococcus epidermidis is a bacterium that is part of the human microbiota. It is most abundant on the skin, in the respiratory system and in the human digestive tract. Also, Staphylococcus aureus contributes to human infections and has a high mortality rate. Both of these bacterial species produce biofilm, a pathogenic factor increasing their resistance to antibiotics. For this reason, we are looking for new substances that can neutralize bacterial cells. One of the best-known substances with such effects are silver nanoparticles. They exhibited antibacterial and antibiofilm formation activity that depended on their size, shape and the concentration used. In this review, we presented the data related to the use of silver nanoparticles in counteracting bacterial growth and biofilm formation published in scientific papers between 2017 and 2021. Based on the review of experimental results, the properties of nanoparticles prompt the expansion of research on their activity.
When Phosphatases Go Mad: The Molecular Basis for Toxicity of Yeast Ppz1
Authors: Casamayor Antonio , Ariño Joaquín
Published: April 2022

The fact that overexpression of the yeast Ser/Thr protein phosphatase Ppz1 induces a dramatic halt in cell proliferation was known long ago, but only work in the last few years has provided insight into the molecular basis for this toxicity. Overexpression of Ppz1 causes abundant changes in gene expression and modifies the phosphorylation state of more than 150 proteins, including key signaling protein kinases such as Hog1 or Snf1. Diverse cellular processes are altered: halt in translation, failure to properly adapt to low glucose supply, acidification of the cytosol, or depletion of intracellular potassium content are a few examples. Therefore, the toxicity derived from an excess of Ppz1 appears to be multifactorial, the characteristic cell growth blockage thus arising from the combination of various altered processes. Notably, overexpression of the Ppz1 regulatory subunit Hal3 fully counteracts the toxic effects of the phosphatase, and this process involves intracellular relocation of the phosphatase to internal membranes.
Mechanisms Involved in Epileptogenesis in Alzheimer’s Disease and Their Therapeutic Implications
Authors: Altuna Miren , Olmedo-Saura Gonzalo , Carmona-Iragui María , Fortea Juan
Published: April 2022

Epilepsy and Alzheimer’s disease (AD) incidence increases with age. There are reciprocal relationships between epilepsy and AD. Epilepsy is a risk factor for AD and, in turn, AD is an independent risk factor for developing epilepsy in old age, and abnormal AD biomarkers in PET and/or CSF are frequently found in late-onset epilepsies of unknown etiology. Accordingly, epilepsy and AD share pathophysiological processes, including neuronal hyperexcitability and an early excitatory–inhibitory dysregulation, leading to dysfunction in the inhibitory GABAergic and excitatory glutamatergic systems. Moreover, both β-amyloid and tau protein aggregates, the anatomopathological hallmarks of AD, have proepileptic effects. Finally, these aggregates have been found in the resection material of refractory temporal lobe epilepsies, suggesting that epilepsy leads to amyloid and tau aggregates. Some epileptic syndromes, such as medial temporal lobe epilepsy, share structural and functional neuroimaging findings with AD, leading to overlapping symptomatology, such as episodic memory deficits and toxic synergistic effects. In this respect, the existence of epileptiform activity and electroclinical seizures in AD appears to accelerate the progression of cognitive decline, and the presence of cognitive decline is much more prevalent in epileptic patients than in elderly patients without epilepsy. Notwithstanding their clinical significance, the diagnosis of clinical seizures in AD is a challenge. Most are focal and manifest with an altered level of consciousness without motor symptoms, and are often interpreted as cognitive fluctuations. Finally, despite the frequent association of epilepsy and AD dementia, there is a lack of clinical trials to guide the use of antiseizure medications (ASMs). There is also a potential role for ASMs to be used as disease-modifying drugs in AD.
Impact of Fungal Spores on Asthma Prevalence and Hospitalization
Authors: Hughes Kira M. , Price Dwan , Torriero Angel A. J. , Symonds Matthew R. E. , Suphioglu Cenk
Published: April 2022

Despite making up a significant proportion of airborne allergens, the relationship between fungal spores and asthma is not fully explored. Only 80 taxa of fungi have so far been observed to exacerbate respiratory presentations, with Cladosporium spp., Aspergillus spp., Penicillium spp., and Alternaria spp. found to comprise the predominant allergenic airborne spores. Fungal spores have been found in indoor environments, such as hospitals and housing due to poor ventilation. Meanwhile, outdoor fungal spores exhibit greater diversity, and higher abundance and have been associated with hospitalizations from acute asthma presentations. In addition, fungal spores may be the underlying, and perhaps the “missing link”, factor influencing the heightened rate of asthma presentations during epidemic thunderstorm asthma events. To improve our knowledge gap on fungal spores, airborne allergen monitoring must be improved to include not only dominant allergenic fungi but also provide real-time data to accurately and quickly warn the general public. Such data will help prevent future asthma exacerbations and thus save lives. In this review, we examine the health risks of prominent allergenic fungal taxa, the factors influencing spore dispersal and distribution, and why improvements should be made to current sampling methods for public health and wellbeing.
Role of Branched-Chain Amino Acid Metabolism in Type 2 Diabetes, Obesity, Cardiovascular Disease and Non-Alcoholic Fatty Liver Disease
Authors: Cuomo Paola , Capparelli Rosanna , Iannelli Antonio , Iannelli Domenico
Published: April 2022

Branched-chain amino acids (BCAAs) include leucine, isoleucine, and valine. Mammalians cannot synthesize these amino acids de novo and must acquire them through their diet. High levels of BCAAs are associated with insulin resistance; type 2 diabetes; obesity; and non-metabolic diseases, including several forms of cancer. BCAAs—in particular leucine—activate the rapamycin complex1 mTORC1, which regulates cell growth and metabolism, glucose metabolism and several more essential physiological processes. Diets rich in BCAAs are associated with metabolic diseases (listed above), while diets low in BCAAs are generally reported to promote metabolic health. As for the dysregulation of the metabolism caused by high levels of BCAAs, recent studies propose that the accumulation of acyl-carnitine and diacyl-CoA in muscles alters lipid metabolism. However, this suggestion is not broadly accepted. On clinical grounds, pre- and post-operative metabolic profiles of candidate patients for bariatric surgery are being used to select the optimal procedure for each individual patient.
A Systematic Review of Ebstein’s Anomaly with Left Ventricular Noncompaction
Authors: Thareja Suma K. , Frommelt Michele A. , Lincoln Joy , Lough John W. , Mitchell Michael E. , Tomita-Mitchell Aoy
Published: April 2022

Traditional definitions of Ebstein’s anomaly (EA) and left ventricular noncompaction (LVNC), two rare congenital heart defects (CHDs), confine disease to either the right or left heart, respectively. Around 15–29% of patients with EA, which has a prevalence of 1 in 20,000 live births, commonly manifest with LVNC. While individual EA or LVNC literature is extensive, relatively little discussion is devoted to the joint appearance of EA and LVNC (EA/LVNC), which poses a higher risk of poor clinical outcomes. We queried PubMed, Medline, and Web of Science for all peer-reviewed publications from inception to February 2022 that discuss EA/LVNC and found 58 unique articles written in English. Here, we summarize and extrapolate commonalities in clinical and genetic understanding of EA/LVNC to date. We additionally postulate involvement of shared developmental pathways that may lead to this combined disease. Anatomical variation in EA/LVNC encompasses characteristics of both CHDs, including tricuspid valve displacement, right heart dilatation, and left ventricular trabeculation, and dictates clinical presentation in both age and severity. Disease treatment is non-specific, ranging from symptomatic management to invasive surgery. Apart from a few variant associations, mainly in sarcomeric genes MYH7 and TPM1, the genetic etiology and pathogenesis of EA/LVNC remain largely unknown.
One Year of COVID-19: Lessons Learned in a Hand Trauma Center
Authors: Covino Marcello , Fulchignoni Camillo , Pietramala Silvia , Barbaliscia Marco , Franceschi Francesco , Maccauro Giulio , Merendi Gianfranco , Rocchi Lorenzo
Published: April 2022

2020 will be remembered worldwide as the year of COVID-19 outbreak. The onset of this pandemic abruptly changed everybody’s life and, in a particular manner, doctors’ lives. Our hand surgery department became rapidly one of the first COVID-19-specialized wards in Italy, impacting considerably the authors’ routines and activities. In this paper, the authors focus on how the demographics of patients with hand trauma changed and how they had to modify their activity. The authors retrospectively took into consideration all patients reaching their emergency department (ED) with hand trauma between 9 March 2020 (the day of the beginning of the first lockdown in Italy) and 8 March 2021 and compared them to those who reached the ED in the three previous years. Authors have analyzed the number of patients, their gender and age, the severity of their trauma, where the trauma occurred, the type of lesion, the percentage of patients who underwent surgery, and the percentage of patients who had an emergency admission. In the last year, the number of patients reaching the ED for a hand trauma has been reduced by two thirds (975 patients during the past year), the mean age of those patients has slightly increased, the severity of cases has increased, places of trauma and type of lesions have changed, and, lastly, the percentage of patients needing surgery who were admitted immediately has increased. This paper shows how the type of patients reaching the ED changed and discusses how surgeons evolved and modified their habits in treating those patients during the first lockdown and the year that followed.
Laboratory Diagnosis of Antiphospholipid Syndrome: Insights and Hindrances
Authors: Vandevelde Arne , Devreese Katrien M. J.
Published: April 2022

Diagnosis of antiphospholipid syndrome (APS) requires the presence of a clinical criterion (thrombosis and/or pregnancy morbidity), combined with persistently circulating antiphospholipid antibodies (aPL). Currently, laboratory criteria aPL consist of lupus anticoagulant (LAC), anticardiolipin antibodies (aCL) IgG/IgM, and anti-β2 glycoprotein I antibodies (aβ2GPI) IgG/IgM. Diagnosis and risk stratification of APS are complex and efforts to standardize and optimize laboratory tests have been ongoing since the initial description of the syndrome. LAC detection is based on functional coagulation assays, while aCL and aβ2GPI are measured with immunological solid-phase assays. LAC assays are especially prone to interference by anticoagulation therapy, but strategies to circumvent this interference are promising. Alternative techniques such as thrombin generation for LAC detection and to estimate LAC pathogenicity have been suggested, but are not applicable yet in routine setting. For aCL and aβ2GPI, a lot of different assays and detection techniques such as enzyme-linked immunosorbent and chemiluminescent assays are available. Furthermore, a lack of universal calibrators or standards results in high variability between the different solid-phase assays. Other non-criteria aPL such as anti-domain I β2 glycoprotein I and antiphosphatidylserine/prothrombin antibodies have been suggested for risk stratification purposes in APS, while their added value to diagnostic criteria seems limited. In this review, we will describe laboratory assays for diagnostic and risk evaluation in APS, integrating applicable guidelines and classification criteria. Current insights and hindrances are addressed with respect to both laboratory and clinical implications.
Management of Epstein–Barr Virus Infection and Post-Transplant Lymphoproliferative Disorder in Pediatric Liver Transplantation
Authors: Okamoto Tatsuya , Okajima Hideaki , Uebayashi Elena Yukie , Ogawa Eri , Yamada Yosuke , Umeda Katsutsugu , Hiramatsu Hidefumi , Hatano Etsurou
Published: April 2022

With the advancement of immunosuppressive strategies, the outcome of liver transplantation during childhood has dramatically improved. On the other hand, Epstein–Barr virus (EBV) infection and associated post-transplant lymphoproliferative diseases (PTLD), such as malignant lymphoma, are serious complications that contribute to morbidity and mortality, and are still an important issue today. Recently, an early diagnosis by quantitative PCR and PET-CT testing, and treatment with rituximab (an anti-CD20 antibody) has been established, and long-term remission has been achieved in many cases. However, the optimal immunosuppression protocol after remission of PTLD needs to be determined, and it is hoped that a treatment for refractory PTLD (e.g., PTL-NOS) will be proposed.
Neurofibromatosis Type 1 Has a Wide Spectrum of Growth Hormone Excess
Authors: Hannah-Shmouni Fady , Trivellin Giampaolo , Beckers Pablo , Karaviti Lefkothea P. , Lodish Maya , Tatsi Christina , Adesina Adekunle M. , Adamidou Fotini , Mintziori Gesthimani , Josefson Jami L. , Quezado Martha , Stratakis Constantine A.
Published: April 2022

Overgrowth due to growth hormone (GH) excess affects approximately 10% of patients with neurofibromatosis type 1 (NF1) and optic pathway glioma (OPG). Our aim is to describe the clinical, biochemical, pathological, and genetic features of GH excess in a retrospective case series of 10 children and adults with NF1 referred to a tertiary care clinical research center. Six children (median age = 4 years, range of 3–5 years), one 14-year-old adolescent, and three adults (median age = 42 years, range of 29–52 years) were diagnosed with NF1 and GH excess. GH excess was confirmed by the failure to suppress GH (<1 ng/mL) on oral glucose tolerance test (OGTT, n = 9) and frequent overnight sampling of GH levels (n = 6). Genetic testing was ascertained through targeted or whole-exome sequencing (n = 9). Five patients (all children) had an OPG without any pituitary abnormality, three patients (one adolescent and two adults) had a pituitary lesion (two tumors, one suggestive hyperplasia) without an OPG, and two patients (one child and one adult) had a pituitary lesion (a pituitary tumor and suggestive hyperplasia, respectively) with a concomitant OPG. The serial overnight sampling of GH levels in six patients revealed abnormal overnight GH profiling. Two adult patients had a voluminous pituitary gland on pituitary imaging. One pituitary tumor from an adolescent patient who harbored a germline heterozygous p.Gln514Pro NF1 variant stained positive for GH and prolactin. One child who harbored a heterozygous truncating variant in exon 46 of NF1 had an OPG that, when compared to normal optic nerves, stained strongly for GPR101, an orphan G protein-coupled receptor causing GH excess in X-linked acrogigantism. We describe a series of patients with GH excess and NF1. Our findings show the variability in patterns of serial overnight GH secretion, somatotroph tumor or hyperplasia in some cases of NF1 and GH excess. Further studies are required to ascertain the link between NF1, GH excess and GPR101, which may aid in the characterization of the molecular underpinning of GH excess in NF1.
Initial Experience with Fenestrated Physician-Modified Stent Grafts Using 3D Aortic Templates
Authors: Rynio Paweł , Jedrzejczak Tomasz , Rybicka Anita , Milner Ross , Gutowski Piotr , Kazimierczak Arkadiusz
Published: April 2022

The goal of this study was to describe the surgical results of physician-modified endografts (PMEG) utilizing a 3D aortic template in a center with no prior experience in complex endovascular aortic repairs. Forty-three patients underwent physician-modified graft stent implantation using a 3D aortic model. The inclusion criteria were juxtarenal and suprarenal aortic aneurysms, type IV thoracoabdominal aneurysms, and type IA endoleak after endovascular aortic repair. In asymptomatic patients, the diameter threshold for aneurysm repair was 5.5 cm in males and 5.0 cm in females. 3D aortic templates were prepared from the patient’s computed tomography angiography scans and sterilized before use in the operating suite. Forty-three stent grafts were modified with the use of a 3D printing template. A total of 162 reinforced fenestrations (37 celiac, 43 right renal, 39 left renal, 43 superior mesenteric) with a mean of 3.8 per patient were performed. All PMEGs had a posterior reducing-diameter tie and a preloaded guidewire. The mean modification time was 86 ± 12 min. The mean follow-up was 14 ± 12 months. The 30-day mortality was 12%. During the follow-up period, the patency rate was 95% per the superior mesenteric artery, 93% per right renal artery, 95% per left renal artery, and 89% per celiac trunk. Twelve (28%) patients had endoleak, of which type I or III was present in 5 (12%) patients, and type II in 7 (16%). 3D printing can be successfully integrated into the physician’s everyday practice of stent graft modification. However, the use of this approach in centers without experience performing complex aortic procedures results in worse surgical metrics than those previously reported.
Relationship among Streptococcus gallolyticus Subsp. gallolyticus, Enterococcus faecalis and Colorectal Neoplasms in Recurrent Endocarditis: A Historical Case Series
Authors: Romay Eva , Pericàs Juan Manuel , García-País María José , Hernández-Meneses Marta , Ayuso Blanca , García-González Javier , Garcés-Durán Rodrigo Vicente , Rabuñal Ramón , Alonso-García Pilar , García-Garrote Fernando , Perissinotti Andrés , Vidal Bàrbara , Falces Carles , Quintana Eduard , Moreira Leticia , Almela Manel , Llach Josep , Moreno Asunción , Corredoira Juan , Miró Jose María ,   on behalf of Lucus Augusti and Hospital Clinic Endocarditis Teams
Published: April 2022

Objectives: The role of colorectal neoplasms (CRN) as a common potential source of recurrent Streptococcus gallolyticus subsp. gallolyticus (SGG) and Enterococcus faecalis (EF) endocarditis remains unstudied. We aimed to investigate what proportion of episodes of recurrent endocarditis are caused by a succession of SGG and EF, or vice versa, and to assess the role of a colonic source in such recurrent episodes. Methods: we conducted a retrospective analysis of two prospective endocarditis cohorts (1979–2019) from two Spanish hospitals, providing descriptive analyses of the major features of the endocarditis episodes, colonoscopy findings, and histologic results. Results: among 1552 IE episodes, 204 (13.1%) were caused by EF and 197 (12.7%) by SGG, respectively. There were 155 episodes (10%) of recurrent IE, 20 of which (12.9%) were due to a succession of SGG/EF IE in 10 patients (the first episode caused by SGG in eight cases, and by EF in two cases). The median follow-up was 86 (interquartile range 34–156) months. In 8/10 initial episodes, the causative microorganism was SGG, and all patients were diagnosed with CRN either during the initial episode or during follow-up. During the second episode of IE or follow-up, colonoscopies revealed CRN in six patients. Conclusions: There seems to be an association between SGG and EF in recurrent endocarditis that warrants further investigation. Our findings reinforce the need for systematically performing colonoscopy in the event of endocarditis caused by both microorganisms.
Using Blockchain for Data Collection in the Automotive Industry Sector: A Literature Review
Authors: Mohammad Abdulghafour , Vargas Sergio , Čermák Pavel
Published: April 2022

Today’s cars can share data with other cars, automakers, and service providers. Shared data can help improve the driving experience, the performance of the car, and the traffic situations. Among all data-collection techniques, blockchain technology offers an immutable and secure solution to support data collection in the automotive industry. Despite its advantages, collecting auto data with blockchain still faces several challenges. Thus, the purpose of this study was to conduct a review of published articles that have addressed the challenges of adopting blockchain for data collection in the automotive industry. This paper allowed us to answer the predefined research question: “What are the challenges of using blockchain for data collection in the automotive industry as presented in the published literature?” The review included articles published from 2017 to January 2022, and from the screened records, 13 articles were analyzed in full-text form. The founded challenges were categorized into seven categories: connectivity, privacy, security attacks, scalability, performance, costs, and monetizing. This review will help researchers, car manufacturers, and third-party suppliers to assess the applicability of the blockchain for data collection.
Real-Time Embedded Implementation of Improved Object Detector for Resource-Constrained Devices
Authors: Ravi Niranjan , El-Sharkawy Mohamed
Published: April 2022

Artificial intelligence (A.I.) has revolutionised a wide range of human activities, including the accelerated development of autonomous vehicles. Self-navigating delivery robots are recent trends in A.I. applications such as multitarget object detection, image classification, and segmentation to tackle sociotechnical challenges, including the development of autonomous driving vehicles, surveillance systems, intelligent transportation, and smart traffic monitoring systems. In recent years, object detection and its deployment on embedded edge devices have seen a rise in interest compared to other perception tasks. Embedded edge devices have limited computing power, which impedes the deployment of efficient detection algorithms in resource-constrained environments. To improve on-board computational latency, edge devices often sacrifice performance, creating the need for highly efficient A.I. models. This research examines existing loss metrics and their weaknesses, and proposes an improved loss metric that can address the bounding box regression problem. Enhanced metrics were implemented in an ultraefficient YOLOv5 network and tested on the targeted datasets. The latest version of the PyTorch framework was incorporated in model development. The model was further deployed using the ROS 2 framework running on NVIDIA Jetson Xavier NX, an embedded development platform, to conduct the experiment in real time.
GPU-Accelerated Multi-Objective Optimal Planning in Stochastic Dynamic Environments
Authors: Chowdhury Rohit , Navsalkar Atharva , Subramani Deepak
Published: April 2022

The importance of autonomous marine vehicles is increasing in a wide range of ocean science and engineering applications. Multi-objective optimization, where trade-offs between multiple conflicting objectives are achieved (such as minimizing expected mission time, energy consumption, and environmental energy harvesting), is crucial for planning optimal routes in stochastic dynamic ocean environments. We develop a multi-objective path planner in stochastic dynamic flows by further developing and improving our recently developed end-to-end GPU-accelerated single-objective Markov Decision Process path planner. MDPs with scalarized rewards for multiple objectives are formulated and solved in idealized stochastic dynamic ocean environments with dynamic obstacles. Three simulated mission scenarios are completed to elucidate our approach and capabilities: (i) an agent moving from a start to target by minimizing travel time and net-energy consumption when harvesting solar energy in an uncertain flow; (ii) an agent moving from a start to target by minimizing travel time and-energy consumption with uncertainties in obstacle initial positions; (iii) an agent attempting to cross a shipping channel while avoiding multiple fast moving ships in an uncertain flow. Optimal operating curves are computed in a fraction of the time that would be required for existing solvers and algorithms. Crucially, our solution can serve as the benchmark for other approximate AI algorithms such as Reinforcement Learning and help improve explainability of those models.
Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma
Authors: Haddad Riad , Zlotnik Oran , Goshen-Lago Tal , Levi Mattan , Brook Elena , Brenner Baruch , Kundel Yulia , Ben-Aharon Irit , Kashtan Hanoch
Published: April 2022

(1) Background: We aimed to explore the association between neoadjuvant treatment, tumor-infiltrating immune lymphocyte (TIL), and tumor-associated macrophage (TAM) and survival in patients with esophageal adenocarcinoma. (2) Methods: Patients who underwent esophagectomy were divided into three groups according to their treatment modality and tumor regression grade (TRG): (i) surgery-only group (SG), (ii) good responders (GR) group (TRG 0–1), and (iii) bad responders (BR) group (TRG 2–3). We then carried out statistical correlations of the immunofluorescence analysis of the immune infiltrate in the esophageal surgical specimens with several clinical and pathological parameters. In addition, we analyzed The Cancer Genomic Atlas (TCGA) dataset for differences in TILs, TAMs, and protein expression in immune pathways. (3) Results: Forty-three patients (SG—15, GR—13, and BR—13) were evaluated. The highest enrichment of CD3+ (p < 0.001), CD8+ (p = 0.001) and CD4+ (p = 0.009) was observed in the stroma of GR patients. On multivariate analysis, only CD8+ T cell and signet-ring features were independent prognostic factors for overall survival. In TCGA analysis, we identified overexpression of TAM and colony-stimulating factor 1 receptor (CSF-1R). (4) Conclusions: High enrichment of lymphocyte subpopulations in the microenvironment of esophageal adenocarcinoma is associated with a favorable response to neoadjuvant treatment and an improved patient outcome.
Enacting Economic Resilience: A Synthesis of Economic and Communication Frameworks
Authors: Betts Timothy , Buzzanell Patrice M.
Published: April 2022

This work examines three frameworks for responding to economic disruption: risk mitigation, systemic recovery, and economic resilience. Specifically, by reviewing the metatheoretical commitments, analytic contexts, and implications of two economic perspectives, represented by risk mitigation and systemic recovery, we argue that current approaches to understanding resilience in academic economics have failed to address ongoing and emergent disruptions in the economic and social world. In response, this work also reviews a possible synthesis of economic and communication frameworks. This review places the economic resilience framework, inspired by the communication theory of resilience, in conversation with extant literature in economics, communication studies, and other disciplines and concludes with an outline for further theoretical, methodological, and practical development.
Evidence for a Strong Relationship between the Cytotoxicity and Intracellular Location of β-Amyloid
Authors: Haque Md. Aminul , Hossain Md. Selim , Bilkis Tahmina , Islam Md. Imamul , Park Il-Seon
Published: April 2022

β-Amyloid (Aβ) is a hallmark peptide of Alzheimer’s disease (AD). Herein, we explored the mechanism underlying the cytotoxicity of this peptide. Double treatment with oligomeric 42-amino-acid Aβ (Aβ42) species, which are more cytotoxic than other conformers such as monomers and fibrils, resulted in increased cytotoxicity. Under this treatment condition, an increase in intracellular localization of the peptide was observed, which indicated that the peptide administered extracellularly entered the cells. The cell-permeable peptide TAT-tagged Aβ42 (tAβ42), which was newly prepared for the study and found to be highly cell-permeable and soluble, induced Aβ-specific lamin protein cleavage, caspase-3/7-like DEVDase activation, and high cytotoxicity (5–10-fold higher than that induced by the wild-type oligomeric preparations). Oligomeric species enrichment and double treatment were not necessary for enhancing the cytotoxicity and intracellular location of the fusion peptide. Taiwaniaflavone, an inhibitor of the cytotoxicity of wild-type Aβ42 and tAβ42, strongly blocked the internalization of the peptides into the cells. These data imply a strong relationship between the cytotoxicity and intracellular location of the Aβ peptide. Based on these results, we suggest that agents that can reduce the cell permeability of Aβ42 are potential AD therapeutics.
Sliding Corrosion Fatigue of Metallic Joint Implants: A Comparative Study of CoCrMo and Ti6Al4V in Simulated Synovial Environments
Authors: Ryu Jae Joong , Cudjoe Edward , Patel Mihir V. , Caputo Matt
Published: April 2022

Mechanical contact in a corrosive synovial environment leads to progressive surface damage at the modular interface of the joint implants. The wear debris and corrosion products degrade the synovial fluids and change the lubrication mechanisms at the joints. Consequently, the unstable joint lubrication and corrosion products will further induce the undesirable performance of the joint implants. In this study, the two major joint materials, CoCrMo and Ti6Al4V, were tested during the course of reciprocal sliding contact in simulated synovial liquids. Open circuit potential and coefficient of friction were monitored to describe electrochemical and mechanical responses. Potentiostatic test results illustrated electrochemical damage on both surfaces that modified oxidation chemistry on both surfaces. However, more significant modification of the CoCrMo surface was detected during wear in the simulated joint liquid. Even with a reduced coefficient of friction on the CoCrMo surface in sodium lactate environments, fretting current density drastically increased in corrosive sodium lactate with pH 2. However, the test results from the Ti6Al4V surface presented less coefficient of friction values, and moderate change in fretting current. Therefore, the experimental study concluded that the biocompatibility of Ti6Al4V is superior to that of CoCrMo in the combined effect of mechanical loadings and an electrochemical environment.
Direct-Ink-Write Printing and Electrospinning of Cellulose Derivatives for Conductive Composite Materials
Authors: Shi Runfeng , Zhang Jiankang , Yang Jinheng , Xu Yanglei , Li Cuihuan , Chen Sheng , Xu Feng
Published: April 2022

The aim of this study is to realize the controlled construction and modulation of micro-/nanostructures of conductive composite materials (CCMs) in a facile way. Herein, interdigital electrodes are prepared by direct-ink-write printing co-blended inks made of ethyl cellulose and carbon nanotubes on cellulose paper. The cellulose nanofibers (CFs) are prepared by electrospinning cellulose acetate on to an aluminum foil, followed by deacetylation in NaOH/ethanol. All co-blended inks exhibit a typical non-Newtonian shear thinning behavior, enabling smooth extrusion and printing. The above electrodes and the conductive CF films with excellent thermal stability are assembled into a pressure sensor, which has a high sensitivity (0.0584 KPa−1) to detect the change in external loading pressure. The obtained porous CFs film is further endowed with conductivity by in situ polymerization of polypyrrole (PPy), which are uniformly distributed on the CFs surface as particles; a triboelectric nanogenerator is constructed by using the CF@PPy film as a tribo-positive friction layer to achieve efficient energy harvesting (output voltage = 29.78 V, output current = 2.12 μA). Therefore, the construction of CCMs with micro-/nanostructures based on cellulose derivatives have essential application prospects in emerging high-tech fields, such as green electronics for sensing and energy harvesting.
Corrosion of NiTiDiscs in Different Seawater Environments
Authors: Pješčić-Šćepanović Jelena , Vastag Gyöngyi , Ivošević Špiro , Kovač Nataša , Rudolf Rebeka
Published: April 2022

This paper gives an approach to the corrosion resistance analysis and changes in the chemical composition of anNiTi alloy in the shape of a disc, depending on different real seawater environments. The NiTi discs were analysed after 6 months of exposure in real seawater environments: the atmosphere, a tidal zone, and seawater. The corrosion tests showed that the highest corrosion rate for the discs is in seawater because this had the highest value of current density, and the initial disc had the most negative potential. Measuring the chemical composition of the discs using inductively coupled plasma and X-ray fluorescence before the experiment and semiquantitative analysis after the experiment showed the chemical composition after 6 months of exposure. Furthermore, the applied principal component analysis and cluster analysis revealed the influence of the different environments on the changes in the chemical composition of the discs. Cluster analysis detected small differences between the similar corrosive influences of the analysed types of environments during the period of exposure. The obtained results confirm that PCA can detect subtle quantitative differences among the corrosive influences of the types of marine environments, although the examined corrosive influences are quite similar. The applied chemometric methods (CA and PCA) are, therefore, sensitive enough to register the existence of slight differences among corrosive environmental influences on the analysed NiTi SMA.
A Numerical Study on the Mesoscopic Characteristics of Ti-6Al-4V by Selective Laser Melting
Authors: Ao Xiaohui , Liu Jianhua , Xia Huanxiong , Yang Ye
Published: April 2022

Selective laser melting is a typical powder-bed additive manufacturing technology, for which it is difficult and expensive to observe and measure the molten pool due to its short lifetime and tiny size. This paper introduced a two-stage mesoscopic layer-by-layer simulation framework for the numerical study of the SLM process, where the powder laying and laser scanning are included and conducted alternatively. For the simulation of powder laying, the dynamic behaviors of the particles as well as the particle–particle and particle–scraper interactions are included. For the simulation of laser scanning, a coupled multi-phase and multi-physics system was considered, where the effects of surface tension, Marangoni effect, and vapor recoil are considered, and the behaviors of heat transfer, fluid flow, and melting/solidification are simulated. This simulation framework was then used to simulate the Ti-6Al-4V SLM process. The evolutions of the molten pool and track were presented, and the characteristics of the molten pool, keyhole, and track were analyzed and discussed, specifically, the effects of the laser power and scanning speed on the three-dimensional morphology and size of the molten pool were numerically studied, and their dependencies were discussed and found.
Many-Scale Investigations of Deformation Behavior of Polycrystalline Composites: II—Micro-Macro Simultaneous FE and Discrete Dislocation Dynamics Simulation
Authors: Schneider Yanling , Rapp Dennis-Michael , Yang Yifang , Wasserbäch Werner , Schmauder Siegfried
Published: April 2022

The current work numerically investigates commercial polycrystalline Ag/17vol.%SnO2 composite tensile deformation behavior with available experimental data. Such composites are useful for electric contacts and have a highly textured initial material status after hot extrusion. Experimentally, the initial sharp fiber texture and the number of Σ3-twins were reduced due to tensile loading. The local inhomogeneous distribution of hardness and Young’s modulus gradually decreased from nanoindentation tests, approaching global homogeneity. Many-scale simulations, including micro-macro simultaneous finite element (FE) and discrete dislocation dynamics (DDD) simulations, were performed. Deformation mechanisms on the microscale are fundamental since they link those on the macro- and nanoscale. This work emphasizes micromechanical deformation behavior. Such FE calculations applied with crystal plasticity can predict local feature evolutions in detail, such as texture, morphology, and stress flow in individual grains. To avoid the negative influence of boundary conditions (BCs) on the result accuracy, BCs are given on the macrostructure, i.e., the microstructure is free of BCs. The particular type of 3D simulation, axisymmetry, is preferred, in which a 2D real microstructural cutout with 513 Ag grains is applied. From FE results, Σ3-twins strongly rotated to the loading direction (twins disappear), which, possibly, caused other grains to rotate away from the loading direction. The DDD simulation treats the dislocations as discrete lines and can predict the resolved shear stress (RSS) inside one grain with dependence on various features as dislocation density and lattice orientation. The RSS can act as the link between the FE and DDD predictions.
A Comprehensive Analysis of the UVC LEDs’ Applications and Decontamination Capability
Authors: Nicolau Talita , Gomes Filho Núbio , Padrão Jorge , Zille Andrea
Published: April 2022

The application of light-emitting diodes (LEDs) has been gaining popularity over the last decades. LEDs have advantages compared to traditional light sources in terms of lifecycle, robustness, compactness, flexibility, and the absence of non-hazardous material. Combining these advantages with the possibility of emitting Ultraviolet C (UVC) makes LEDs serious candidates for light sources in decontamination systems. Nevertheless, it is unclear if they present better decontamination effectiveness than traditional mercury vapor lamps. Hence, this research uses a systematic literature review (SLR) to enlighten three aspects: (1) UVC LEDs’ application according to the field, (2) UVC LEDs’ application in terms of different biological indicators, and (3) the decontamination effectiveness of UVC LEDs in comparison to conventional lamps. UVC LEDs have spread across multiple areas, ranging from health applications to wastewater or food decontamination. The UVC LEDs’ decontamination effectiveness is as good as mercury vapor lamps. In some cases, LEDs even provide better results than conventional mercury vapor lamps. However, the increase in the targets’ complexity (e.g., multilayers or thicker individual layers) may reduce the UVC decontamination efficacy. Therefore, UVC LEDs still require considerable optimization. These findings are stimulating for developing industrial or final users’ applications.
Can a Black-Box AI Replace Costly DMA Testing?—A Case Study on Prediction and Optimization of Dynamic Mechanical Properties of 3D Printed Acrylonitrile Butadiene Styrene
Authors: Vahed Ronak , Zareie Rajani Hamid R. , Milani Abbas S.
Published: April 2022

The complex and non-linear nature of material properties evolution during 3D printing continues to make experimental optimization of Fused Deposition Modeling (FDM) costly, thus entailing the development of mathematical predictive models. This paper proposes a two-stage methodology based on coupling limited data experiments with black-box AI modeling and then performing heuristic optimization, to enhance the viscoelastic properties of FDM processed acrylonitrile butadiene styrene (ABS). The effect of selected process parameters (including nozzle temperature, layer height, raster orientation and deposition speed) as well as their combinative effects are also studied. Specifically, in the first step, a Taguchi orthogonal array was employed to design the Dynamic Mechanical Analysis (DMA) experiments with a minimal number of runs, while considering different working conditions (temperatures) of the final prints. The significance of process parameters was measured using Lenth’s statistical method. Combinative effects of FDM parameters were noted to be highly nonlinear and complex. Next, artificial neural networks were trained to predict both the storage and loss moduli of the 3D printed samples, and consequently, the process parameters were optimized via Particle Swarm Optimization (PSO). The optimized process of the prints showed overall a closer behavior to that of the parent (unprocessed) ABS, when compared to the unoptimized set-up.