bluuebunny/crossref_metadata_2025 huggingface dataset:
The City of Poetry
Authors: Lummus David
Published: December 2020

What did it mean to be a poet in fourteenth-century Italy? What counted as poetry? In an effort to answer these questions, this book examines the careers of four medieval Italian poets (Albertino Mussato, Dante Alighieri, Francesco Petrarch, and Giovanni Boccaccio) who wrote in both Latin and the Italian vernacular. In readings of defenses of poetry, speeches and letters on public laurel-crowning ceremonies, and other theoretical and poetic texts, this book shows how these poets viewed their authorship of poetic works as a function of their engagement in a human community. Each poet represents a model of the poet as a public intellectual - a poet-theologian - who can intervene in public affairs thanks to his authority within texts. The City of Poetry provides a new historicized approach to understanding poetic culture in fourteenth-century Italy which reshapes long-standing Romantic views of poetry as a timeless and sublimely inspired form of discourse.
Limit theorems for multi-type general branching processes with population dependence
Authors: Fan Jie Yen , Hamza Kais , Jagers Peter , Klebaner Fima C.
Published: December 2020

A general multi-type population model is considered, where individuals live and reproduce according to their age and type, but also under the influence of the size and composition of the entire population. We describe the dynamics of the population as a measure-valued process and obtain its asymptotics as the population grows with the environmental carrying capacity. Thus, a deterministic approximation is given, in the form of a law of large numbers, as well as a central limit theorem. This general framework is then adapted to model sexual reproduction, with a special section on serial monogamic mating systems.
Corrigendum to: Asymptotic shape and the speed of propagation of continuous-time continuous-space birth processes
Authors: Bezborodov V.
Published: December 2020

We would like to correct the statement of Lemma 4.1 in [BDK+18].
Protection of the Rights of Employees in Insolvency Law: A Zimbabwean Perspective
Authors: Kasuso Tapiwa Givemore , Sithole Kudakwashe
Published: December 2020

Until recently, Zimbabwean insolvency law was unconcerned with rights of employees on insolvency of the employer. The new Insolvency Act points in a different direction. It guarantees limited rights of workers in their capacity as creditors and as employees. There is a convergence of insolvency law and labour law. These are legal disciplines with contradictory philosophies. This contribution analyses the rights of employees on insolvency in Zimbabwe. The review is informed by international best practices. The article establishes that Zimbabwe follows the “model two: bankruptcy preference approach”. It brings to the fore fundamental weaknesses inherent with this approach in the Zimbabwean context. The article argues that the protection of employees’ rights on insolvency can be enhanced if Zimbabwe follows the “pro-employee approach” and the “bankruptcy priority-guarantee fund approach”. It concludes by advocating for the alignment of the Insolvency Act with international best practices, the constitution and labour legislation.
Association between socioeconomic indicators and geographic distribution of vestibular schwannomas in West Scotland: a 15-year review
Authors: Caulley L , Sawada M , Crighton E , Khoury M , Kontorinis G
Published: December 2020

Objective

Socioeconomic risk factors may contribute to geographic variation in diseases, but studies are limited due to lack of large available cohorts.

Method

A geographic analysis was performed of the association between socioeconomic risk factors and the distribution of vestibular schwannomas in adults diagnosed with sporadic vestibular schwannomas through the National Health Services in the West of Scotland from 2000 to 2015.

Results

A total of 511 sporadic vestibular schwannomas were identified in a population of over 3.1 million. Prevalence of vestibular schwannomas were lowest in cases with good health (–0.64, 95 per cent confidence interval: –0.93,–0.38; p = 0.002) and level 1 qualifications (–0.562, 95 per cent confidence interval: –0.882 to –0.26; p = 0.01). However, these risk factors did not demonstrate consistent linearity of correlations. Prevalence was lower in people originating from European Union accession countries from April 2001 to March 2011 (–0.63, 95 per cent confidence interval: –0.84 to –0.43; p = 0.002). No correlation between distribution of vestibular schwannomas and socioeconomic risk factors met our threshold criteria (± 0.7).

Conclusion

This study demonstrated that there is little variation in distribution of vestibular schwannomas by socioeconomic risk factors.
Optimized phasing conditions to avoid edge mode excitation by ICRH antennas
Authors: Maquet V. , Messiaen A.
Published: December 2020

An ion cyclotron resonance heating (ICRH) antenna system must launch radio frequency (RF) power with a wavenumber spectrum which maximizes the coupling to the plasma. It should also ensure good absorption while minimizing the wave interaction with the plasma edge. Such interactions lead to impurity release, whose effect has been measured far from the antenna location (Klepper et al. 2013; Wukitch et al. 2017; Perkins et al. 2019) and can involve the entire scrape-off layer. In the normal heating scenario, for which the frequency of the waves launched by the antenna is larger than the ion cyclotron frequency of the majority ions

$\omega > \omega _{\textrm {ci},\textrm {maj}}$

, release of impurities due to ICRH can be affected by minimizing the low

$|k_{\parallel }| < k_0$

power spectrum components of the antenna. Impurity release can be the result of low central absorption of the waves or power transfer from the fast to the slow wave due to the presence of a confluence in the plasma edge. In ASDEX Upgrade (AUG), a reduction of heavy impurity release by ICRH in the plasma was qualitatively well correlated to the parallel electric field and RF currents flowing around the antenna (Bobkov et al. 2017). In this article, we first show a correlation between the reduction in impurity release by ICRH in AUG and the rejection of the low

$|k_{\parallel }| < k_0$

region of the antenna power spectrum. We show that the same correlation holds for results obtained in the Alcator C-Mod tokamak. Finally, using this idea, we reproduce ICRH induced impurity release behaviour in a not yet published experiments of JET, and make predictions for ITER and DEMO.
Are atheist worlds really the best?
Authors: LOUGHEED KIRK
Published: December 2020

Anti-theism is the view that God's existence would (or does) detract from the value of the world. A distinctive argument for anti-theism says that the very best atheist worlds are better than the best theist worlds. The reason for this is that it's possible to gain most or all of the benefits associated with theism in Godless worlds. For instance, worlds with a lesser god or several lesser gods can provide many of the benefits of theism without the associated disadvantages. While some work has been done to show that the advantages of theism can be had in atheist worlds, very little has been said regarding whether the disadvantages can simultaneously be avoided in such worlds. I aim to show that it is difficult to describe a possible atheist world where the benefits of theism obtain but the harms do not. I thus problematize this particular argument for anti-theism.
Cost-effectiveness analysis of magnetic resonance-guided focused ultrasound ablation for palliation of refractory painful bone metastases
Authors: Bucknor Matthew D. , Chan Frandics P. , Matuoka Jessica Y. , Curl Patti K. , Kahn James G.
Published: December 2020

Objective

The aim of this study was to determine if magnetic resonance-guided focused ultrasound (MRgFUS) is cost-effective compared with medication, for refractory pain from bone metastases in the United States.

Methods

We constructed a Markov state transition model using TreeAge Pro software (TreeAge Software, Inc., Williamstown, MA, USA) to model costs, outcomes, and the cost-effectiveness of a treatment strategy using MRgFUS for palliative treatment of painful bone metastases compared with a Medication Only strategy (Figure 1). Model transition state probabilities, costs (in 2018 US$), and effectiveness data (quality-adjusted life-years [QALYs]) were derived from available literature, local expert opinion, and reimbursement patterns at two U.S. tertiary academic medical centers actively performing MRgFUS. Costs and QALYs, discounted at three percent per year, were accumulated each month over a 24-month time horizon. One-way and probabilistic sensitivity analyses were performed.

Results

In the base-case analysis, the MRgFUS treatment strategy costs an additional $11,863 over the 2-year time horizon to accumulate additional 0.22 QALYs, equal to a $54,160/QALY ICER, thus making MRgFUS the preferred strategy. One-way sensitivity analyses demonstrate that for the base-case analysis, the crossover point at which Medication Only would instead become the preferred strategy is $23,341 per treatment. Probabilistic sensitivity analyses demonstrate that 67 percent of model iterations supported the conclusion of the base case.

Conclusions

Our model demonstrates that MRgFUS is cost-effective compared with Medication Only for palliation of painful bone metastases for patients with medically refractory metastatic bone pain across a range of sensitivity analyses.
Representing the Unrepresentable in South Korean Activist Performances
Authors: Jeong Areum
Published: December 2020

On 16 April 2014, the Sewol Ferry capsized in the southern region of South Korea: 304 passengers died, including 250 high school students. Despite an international outcry, there has not yet been a comprehensive investigation into what caused the Sewol to sink and why the passengers were not rescued promptly. This article discusses how performance can represent something that defies explanation because we do not know how or why it happened. Yellow Ribbon’s Talent Show, Namsan Arts Centre’s From Pluto, and Camino de Ansan performed the role of the students who died. Taking these three case studies, this article analyzes the ways in which they strive to represent the unrepresentable as they attempt to document the sinking and achieve justice, while memorializing the victims and arguing for the necessity of a more safety-conscious society. Areum Jeong is Assistant Professor in Humanities at Sichuan University-Pittsburgh Institute. Jeong’s research takes a transnational approach to Korean and Korean-American film, literature, theatre, and performance. Her current book project explores how performance documents death, loss, and memory in South Korean and diasporic communities.
The origins and development of the Cardiac Neurodevelopmental Outcome Collaborative: creating innovative clinical, quality improvement, and research opportunities
Authors: Marino Bradley S. , Sood Erica , Cassidy Adam R. , Miller Thomas A. , Sanz Jacqueline H. , Bellinger David , Newburger Jane , Goldberg Caren S.
Published: December 2020

Compared to the general population, individuals with complex congenital heart disease are at increased risk for deficits in cognitive, neurodevelopmental, psychosocial, and physical functioning, resulting in a diminished health-related quality of life. These deficits have been well described over the past 25 years, but significant gaps remain in our understanding of the best practices to improve neurodevelopmental and psychosocial outcomes and health-related quality of life for individuals with paediatric and congenital heart disease. Innovative clinical, quality improvement, and research opportunities with collaboration across multiple disciplines and institutions were needed to address these gaps. The Cardiac Neurodevelopmental Outcome Collaborative was founded in 2016 with a described mission to determine and implement best practices of neurodevelopmental and psychosocial services for individuals and their families with paediatric and congenital heart disease through clinical, quality improvement, and research initiatives. The vision is to be a multi-centre, multi-national, multi-disciplinary group of healthcare professionals committed to working together and partnering with families to optimise neurodevelopmental outcomes for individuals with paediatric and congenital heart disease through clinical, quality, and research initiatives, intending to maximise quality of life for every individual across the lifespan. This manuscript describes the development and organisation of the Cardiac Neurodevelopmental Outcome Collaborative.
Herbicide symptomology and the mechanism of action of methiozolin
Authors: Brabham Chad , Johnen Philipp , Hendriks Janneke , Betz Michael , Zimmermann Alexandra , Gollihue Jarrad , Serson William , Kempinski Chase , Barrett Michael
Published: December 2020

Methiozolin is a new herbicide with an unknown mechanism of action (MOA) for control of annual bluegrass (Poa annua L.) in several warm- and cool-season turfgrasses. In the literature, methiozolin was proposed to be a pigment inhibitor via inhibition of tyrosine aminotransferases (TATs) or a cellulose biosynthesis inhibitor (CBI). Here, exploratory research was conducted to characterize the herbicide symptomology and MOA of methiozolin. Arabidopsis (Arabidopsis thaliana L.) and P. annua exhibited a similar level of susceptibility to methiozolin, and arrest of meristematic growth was the most characteristic symptomology. For example, methiozolin inhibited A. thaliana root growth (GR50 8 nM) and shoot emergence (GR80 ˜50 nM), and apical meristem growth was completely arrested at rates greater than 500 nM. We concluded that methiozolin was neither a TAT nor a CBI inhibitor. Methiozolin had a minor effect on chlorophyll and alpha-tocopherol content in treated seedlings (<500 nM), and supplements in the proposed TAT pathway could not lessen phytotoxicity. Examination of microscopic images of roots revealed that methiozolin-treated (100 nM) and untreated seedlings had similar root cell lengths. Thus, methiozolin inhibits cell proliferation and not elongation from meristematic tissue. Subsequently, we suspected methiozolin was an inhibitor of the mevalonic acid (MVA) pathway, because its herbicidal symptomologies were nearly indistinguishable from those caused by lovastatin. However, methiozolin did not inhibit phytosterol production, and MVA pathway metabolites did not rescue treated seedlings. Further experiments showed that methiozolin produced a physiological profile very similar to cinmethylin across a number of assays, a known inhibitor of fatty-acid synthesis through inhibition of thioesterases (FATs). Experiments with lesser duckweed (Lemna aequinoctialis Welw.; syn. Lemna paucicostata Hegelm.) showed that methiozolin also reduced fatty-acid content in Lemna with a profile similar, but not identical, to cinmethylin. However, there was no difference in fatty-acid content between treated (1 µM) and untreated A. thaliana seedlings. Methiozolin also bound to both A, thaliana and L. aequinoctialis FATs in vitro. Modeling suggested that methiozolin and cinmethylin have comparable and overlapping FAT binding sites. While there was a discrepancy in the effect of methiozolin on fatty-acid content between L. aequinoctialis and A. thaliana, the overall evidence indicates that methiozolin is a FAT inhibitor and acts in a similar manner as cinmethylin.
Comparing Spatial and Temporal Variation of Lake‐Atmosphere Carbon Dioxide Fluxes Using Multiple Methods
Authors: Baldocchi Angela K. , Reed David E. , Loken Luke C. , Stanley Emily H. , Huerd Hayley , Desai Ankur R.
Published: December 2020

Lakes emit globally significant amounts of carbon dioxide (CO2) to the atmosphere, but quantifying these rates for individual lakes is extremely challenging. The exchange of CO2 across the air‐water interface is driven by physical, chemical, and biological processes in both the lake and the atmosphere that vary at multiple spatial and temporal scales. None of the methods we use to estimate CO2 flux fully capture this heterogeneous gas exchange. Here, we compared concurrent CO2 flux estimates from a single lake based on commonly used methods. These include floating chambers (FCs), eddy covariance (EC), and two concentration gradient‐based methods labeled fixed (F‐pCO₂) and spatial (S‐pCO₂). At the end of summer, cumulative carbon fluxes were similar between EC, F‐pCO₂, and S‐pCO₂ methods (−4, −4, and −9.5 gC m−2), while methods diverged in directionality of fluxes during the fall turnover period (−50, 43, and 38 gC m−2). Collectively, these results highlight the discrepancies among methods and the need to acknowledge the uncertainty when using any of them to approximate this heterogeneous flux.
A Pandemic Pivot in Earth Science Outreach and Education
Authors: Hubenthal Michael , Bohon Wendy , Taber John
Published: December 2020

For some educators, restrictions imposed by COVID-19 created space for innovation and affirmed the value of online tools and learning environments for increasing access to and engagement with science.
Satellite Monitoring of Global Surface Soil Organic Carbon Dynamics Using the SMAP Level 4 Carbon Product
Authors: Arthur Endsley K. , Kimball John S. , Reichle Rolf H. , Watts Jennifer D.
Published: December 2020

Soil organic carbon (SOC) is an important metric of soil health and the terrestrial carbon balance. Short‐term climate variations affect SOC through changes in temperature and moisture, which control vegetation growth and soil decomposition. We evaluated a satellite data‐driven carbon model, operating under the NASA Soil Moisture Active‐Passive (SMAP) mission, as a means of monitoring global surface SOC dynamics. The SMAP Level 4 Carbon (L4C) product estimates a daily global carbon budget including surface (0‐ to 5‐cm depth) SOC. We found that the L4C mean latitudinal SOC distribution is generally consistent with alternative assessments from static soil inventory records and dynamic global vegetation models (r ≥ 0.89). Within forest systems, based on inventory data, L4C SOC is most similar in magnitude to litterfall but is correlated with coarse woody debris (
) and total SOC (
). L4C SOC is sensitive to seasonal and annual climate variability, with mean residence times that range from 1.5 years in the wet tropics to 17 years in the cold tundra. Incorporating soil moisture retrievals from the SMAP L‐band (1.4 GHz) microwave radiometer within the L4C algorithm provides enhanced soil moisture sensitivity under low‐to‐moderate vegetation cover (<5 kg m−2 vegetation water content). The L‐band soil moisture had the greatest impact on the L4C carbon budget in semiarid regions, which span almost 60% of the globe and account for substantial variability in the terrestrial carbon sink. The L4C operational product enables prognostic investigations into effects of recent climate trends and anomalies (e.g., droughts and pluvials) on shallow soil carbon dynamics.
Correlation of anti-tumour drug resistance with epigenetic regulation
Authors: Hayashi Takuma , Konishi Ikuo
Published: December 2020

During treatment, the development of drug-resistant tumours remains an important challenge in cancer treatment. Epigenetic changes have been reported as one of the mechanisms for anti-tumour drug resistance. In clinical practice, a combination of epigenetic-related drugs can be considered as a future selection of cancer therapeutic drugs.
A novel mutation in the ACAN gene in a family with autosomal dominant short stature and intervertebral disc disease
Authors: Uchida Noboru , Shibata Hironori , Nishimura Gen , Hasegawa Tomonobu
Published: December 2020

Heterozygous mutations in the ACAN gene have been reported in individuals with short stature and advanced bone age, with or without early-onset osteoarthritis and/or osteochondritis dissecans. We report a family with a phenotypic constellation carrying a novel mutation in the ACAN gene. The proband was a 7-year-old Japanese girl with short stature. Her mother and maternal grandmother also had short stature and intervertebral disc disease. We analyzed the ACAN gene in the family and identified a novel heterozygous mutation: c.4634delT, Leu1545Profs*11.
MassArray analysis of genomic susceptibility variants in ovarian cancer
Authors: Verma Sonali , Sharma Indu , Sharma Varun , Bhat Amrita , Shah Ruchi , Bhat Gh. Rasool , Sharma Bhanu , Bakshi Divya , Nagpal Ashna , Wakhloo Ajay , Bhat Audesh , Kumar Rakesh
Published: December 2020

Ovarian cancer (OC), a multifaceted and genetically heterogeneous malignancy is one of the most common cancers among women. The aim of the study is to unravel the genetic factors associated with OC and the extent of genetic heterogeneity in the populations of Jammu and Kashmir (J&K).Using the high throughput Agena MassARRAY platform, present case control study was designed which comprises 200 histopathological confirmed OC patients and 400 age and ethnicity matched healthy controls to ascertain the association of previously reported eleven single nucleotide polymorphisms (SNPs) spread over ten genes (DNMT3A, PIK3CA, FGFR2, GSTP1, ERCC5, AKT1, CASC16, CYP19A1, BCL2 and ERCC1) within the OC population of Jammu and Kashmir, India. The association of each variant was estimated using logistic regression analyses. Out of the 11 SNPs the odds ratio observed for three SNPs; rs2699887 was (1.72 at 95% CI: 1.19–2.48, p = 0.004), rs1695 was (1.87 at 95% CI: 1.28–2.71, p = 0.001), and rs2298881 was (0.66 at 95% CI: 0.46–0.96, p = 0.03) were found significantly associated with the OC after correction with confounding factors i.e. age & BMI. Furthermore, the estimation of interactive analyses was performed and odds ratio observed was 2.44 (1.72–3.47), p value < 0. 001 suggests that there was a strong existence of interplay between the selected genetic variants in OC, which demonstrate that interactive analysis highlights the role of gene–gene interaction that provides an insight among multiple little effects of various polymorphisms in OC.
The psychological correlates of distinct neural states occurring during wakeful rest
Authors: Karapanagiotidis Theodoros , Vidaurre Diego , Quinn Andrew J. , Vatansever Deniz , Poerio Giulia L. , Turnbull Adam , Ho Nerissa Siu Ping , Leech Robert , Bernhardt Boris C. , Jefferies Elizabeth , Margulies Daniel S. , Nichols Thomas E. , Woolrich Mark W. , Smallwood Jonathan
Published: December 2020

When unoccupied by an explicit external task, humans engage in a wide range of different types of self-generated thinking. These are often unrelated to the immediate environment and have unique psychological features. Although contemporary perspectives on ongoing thought recognise the heterogeneity of these self-generated states, we lack both a clear understanding of how to classify the specific states, and how they can be mapped empirically. In the current study, we capitalise on advances in machine learning that allow continuous neural data to be divided into a set of distinct temporally re-occurring patterns, or states. We applied this technique to a large set of resting state data in which we also acquired retrospective descriptions of the participants’ experiences during the scan. We found that two of the identified states were predictive of patterns of thinking at rest. One state highlighted a pattern of neural activity commonly seen during demanding tasks, and the time individuals spent in this state was associated with descriptions of experience focused on problem solving in the future. A second state was associated with patterns of activity that are commonly seen under less demanding conditions, and the time spent in it was linked to reports of intrusive thoughts about the past. Finally, we found that these two neural states tended to fall at either end of a neural hierarchy that is thought to reflect the brain’s response to cognitive demands. Together, these results demonstrate that approaches which take advantage of time-varying changes in neural function can play an important role in understanding the repertoire of self-generated states. Moreover, they establish that important features of self-generated ongoing experience are related to variation along a similar vein to those seen when the brain responds to cognitive task demands.
Peristaltic flow in the glymphatic system
Authors: Romanò Francesco , Suresh Vinod , Galie Peter A. , Grotberg James B.
Published: December 2020

The flow inside the perivascular space (PVS) is modeled using a first-principles approach in order to investigate how the cerebrospinal fluid (CSF) enters the brain through a permeable layer of glial cells. Lubrication theory is employed to deal with the flow in the thin annular gap of the perivascular space between an impermeable artery and the brain tissue. The artery has an imposed peristaltic deformation and the deformable brain tissue is modeled by means of an elastic Hooke’s law. The perivascular flow model is solved numerically, discovering that the peristaltic wave induces a steady streaming to/from the brain which strongly depends on the rigidity and the permeability of the brain tissue. A detailed quantification of the through flow across the glial boundary is obtained for a large parameter space of physiologically relevant conditions. The parameters include the elasticity and permeability of the brain, the curvature of the artery, its length and the amplitude of the peristaltic wave. A steady streaming component of the through flow due to the peristaltic wave is characterized by an in-depth physical analysis and the velocity across the glial layer is found to flow from and to the PVS, depending on the elasticity and permeability of the brain. The through CSF flow velocity is quantified to be of the order of micrometers per seconds.
Detection of embedded dynamics in the Györgyi-Field model
Authors: Buchlovská Nagyová Judita , Jansík Branislav , Lampart Marek
Published: December 2020

The main aim of this paper is to detect embedded dynamics of the Györgyi-Field model of the Belousov–Zhabotinsky chemical reaction. The corresponding three-variable model given as a set of nonlinear ordinary differential equations depends on one parameter, the flow rate. As certain values of this parameter can give rise to chaos, an analysis was performed in order to identify different dynamics regimes. Dynamical properties were qualified and quantified using classical and also new techniques; namely, phase portraits, bifurcation diagrams, the Fourier spectra analysis, the 0–1 test for chaos, approximate entropy, and the maximal Lyapunov exponent. The correlation between approximate entropy and the 0–1 test for chaos was observed and described in detail. The main discovery was that the three-stage system of nested sub-intervals of flow rates showed the same pattern in the 0–1 test for chaos and approximate entropy at every level. The investigation leads to the open problem of whether the set of flow rate parameters has Cantor-like structure.
Multiplicity of carotene patterns derives from competition between phytoene desaturase diversification and biological environments
Authors: Fournié Mathieu , Truan Gilles
Published: December 2020

Phytoene desaturases catalyse from two to six desaturation reactions on phytoene, generating a large diversity of molecules that can then be cyclised and produce, depending on the organism, many different carotenoids. We constructed a phylogenetic tree of a subset of phytoene desaturases from the CrtI family for which functional data was available. We expressed in a bacterial system eight codon optimized CrtI enzymes from different clades. Analysis of the phytoene desaturation reactions on crude extracts showed that three CrtI enzymes can catalyse up to six desaturations, forming tetradehydrolycopene. Kinetic data generated using a subset of five purified enzymes demonstrate the existence of characteristic patterns of desaturated molecules associated with various CrtI clades. The kinetic data was also analysed using a classical Michaelis–Menten kinetic model, showing that variations in the reaction rates and binding constants could explain the various carotene patterns observed. Competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full β-carotene production pathway. Our results demonstrate that the desaturation patterns of carotene molecules in various biological environments cannot be fully inferred from phytoene desaturases classification but is governed both by evolutionary-linked variations in the desaturation rates and competition between desaturation and cyclisation steps.
A semi-automated quantitative comparison of metal artifact reduction in photon-counting computed tomography by energy-selective thresholding
Authors: Do T. D. , Sawall S. , Heinze S. , Reiner T. , Ziener C. H. , Stiller W. , Schlemmer H. P. , Kachelrieß M. , Kauczor H. U. , Skornitzke S.
Published: December 2020

An evaluation of energy thresholding and acquisition mode for metal artifact reduction in Photon-counting detector CT (PCD-CT) compared to conventional energy-integrating detector CT (EID-CT) was performed. Images of a hip prosthesis phantom placed in a water bath were acquired on a scanner with PCD-CT and EID-CT (tube potentials: 100, 120 and 140 kVp) and energy thresholds (above 55–75 keV) in Macro and Chess mode. Only high-energy threshold images (HTI) were used. Metal artifacts were quantified by a semi-automated segmentation algorithm, calculating artifact volumes, means and standard deviations of CT numbers. Images of a human cadaver with hip prosthesis were acquired on the PCD-CT in Macro mode as proof-of-concept. Images at 140 kVp showed less metal artifacts than 120 kVp or 100 kVp. HTI (70, 75 keV) had fewer artifacts than low energy thresholds (55, 60, 65 keV). Fewer artifacts were observed in the Macro-HTI (8.9–13.3%) for cortical bone compared to Chess-HTI (9.4–19.1%) and EID-CT (10.7–19.0%) whereas in bone marrow Chess-HTI (19.9–45.1%) showed less artifacts compared to Macro-HTI (21.9–38.3%) and EID-CT (36.4–54.9%). Noise for PCD-CT (56–81 HU) was higher than EID-CT (33–36 HU) irrespective of tube potential. High-energy thresholding could be used for metal artifact reduction in PCD-CT, but further investigation of acquisition modes depending on target structure is required.
HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis
Authors: Katanaev Vladimir L. , Kryuchkov Mikhail , Averkov Volodymyr , Savitsky Mikhail , Nikolaeva Kseniya , Klimova Nadezhda , Khaustov Sergei , Solis Gonzalo P.
Published: December 2020

Drosophila melanogaster has been a model for multiple human disease conditions, including cancer. Among Drosophila tissues, the eye development is particularly sensitive to perturbations of the embryonic signaling pathways, whose improper activation in humans underlies various forms of cancer. We have launched the HumanaFly project, whereas human genes expressed in breast cancer patients are screened for their ability to aberrate development of the Drosophila eye, hoping to thus identify novel oncogenes. Here we report identification of a breast cancer transgene, which upon expression in Drosophila produces eye malformation similar to the famous Glazed phenotype discovered by Thomas Morgan and decades later dissected to originate from mis-expression of Wingless (Wg). Wg is the ortholog of human Wnt proteins serving as ligands to initiate the developmental/oncogenic Wnt signaling pathway. Through genetic experiments we identified that this transgene interacted with the Wg production machinery, rather than with Wg signal transduction. In Drosophila imaginal discs, we directly show that the transgene promoted long-range diffusion of Wg, affecting expression of the Wg target genes. The transgene emerged to encode RPS12—a protein of the small ribosomal subunit overexpressed in several cancer types and known to also possess extra-ribosomal functions. Our work identifies RPS12 as an unexpected regulator of secretion and activity of Wnts. As Wnt signaling is particularly important in the context of breast cancer initiation and progression, RPS12 might be implicated in tumorigenesis in this and other Wnt-dependent cancers. Continuation of our HumanaFly project may bring further discoveries on oncogenic mechanisms.
Fall-related functional impairments in patients with neurological gait disorder
Authors: Ehrhardt Angela , Hostettler Pascal , Widmer Lucas , Reuter Katja , Petersen Jens Alexander , Straumann Dominik , Filli Linard
Published: December 2020

Falls are common in patients with neurological disorders and are a primary cause of injuries. Nonetheless, fall-associated gait characteristics are poorly understood in these patients. Objective, quantitative gait analysis is an important tool to identify the principal fall-related motor characteristics and to advance fall prevention in patients with neurological disorders. Fall incidence was assessed in 60 subjects with different neurological disorders. Patients underwent a comprehensive set of functional assessments including instrumented gait analysis, computerized postural assessments and clinical walking tests. Determinants of falls were assessed by binary logistic regression analysis and receiver operator characteristics (ROC). The best single determinant of fallers was a step length reduction at slow walking speed reaching an accuracy of 67.2% (ROC AUC: 0.669; p = 0.027). The combination of 4 spatio-temporal gait parameters including step length and parameters of variability and asymmetry were able to classify fallers and non-fallers with an accuracy of 81.0% (ROC AUC: 0.882; p < 0.001). These findings suggest significant differences in specific spatio-temporal gait parameters between fallers and non-fallers among neurological patients. Fall-related impairments were mainly identified for spatio-temporal gait characteristics, suggesting that instrumented, objective gait analysis is an important tool to estimate patients' fall risk. Our results highlight pivotal fall-related walking deficits that might be targeted by future rehabilitative interventions that aim at attenuating falls.
Blattella germanica displays a large arsenal of antimicrobial peptide genes
Authors: Silva Francisco J. , Muñoz-Benavent Maria , García-Ferris Carlos , Latorre Amparo
Published: December 2020

Defence systems against microbial pathogens are present in most living beings. The German cockroach Blattella germanica requires these systems to adapt to unhealthy environments with abundance of pathogenic microbes, in addition to potentially control its symbiotic systems. To handle this situation, four antimicrobial gene families (defensins, termicins, drosomycins and attacins) were expanded in its genome. Remarkably, a new gene family (blattellicins) emerged recently after duplication and fast evolution of an attacin gene, which is now encoding larger proteins with the presence of a long stretch of glutamines and glutamic acids. Phylogenetic reconstruction, within Blattellinae, suggests that this duplication took place before the divergence of Blattella and Episymploce genera. The latter harbours a long attacin gene (pre-blattellicin), but the absence of the encoded Glx-region suggests that this element evolved recently in the Blattella lineage. A screening of AMP gene expression in available transcriptomic SR projects of B. germanica showed that, while some AMPs are expressed during almost the whole development, others are restricted to shorter periods. Blattellicins are highly expressed only in adult females. None of the available SR tissue projects could be associated with blattellicins’ expression, suggesting that it takes place in other tissues, maybe the gut.
A novel reverse two-hybrid method for the identification of missense mutations that disrupt protein–protein binding
Authors: Vincent Olivier , Gutierrez-Nogués Angel , Trejo-Herrero Adrían , Navas María-Angeles
Published: December 2020

The reverse two-hybrid system is a powerful method to select mutations that disrupt the interaction between two proteins and therefore to identify the residues involved in this interaction. However, the usefulness of this technique has been limited by its relative complexity when compared to the classical two-hybrid system, since an additional selection step is required to eliminate the high background of uninformative truncation mutants. We have developed a new method that combines the classical and reverse two-hybrid systems to select loss-of-binding missense mutations in a single step. The strategy used to select against truncation mutants is based on the two-hybrid interaction between a C-terminal fusion peptide and the Tsg101 protein. We have applied this method to identify mutations in human glucokinase (GK) that disrupt glucokinase regulatory protein (GKRP) binding. Our results indicate that this method is very efficient and eliminates all the truncation mutants and false positives. The mutated residues identified in GK are involved in the GKRP binding interface or in stabilizing the super-open conformation of GK that binds GKRP. This technique offers an improvement over existing methods in terms of speed, efficiency and simplicity and can be used to study any detectable protein interaction in the two-hybrid system.
Linking diverse salinity responses of 14 almond rootstocks with physiological, biochemical, and genetic determinants
Authors: Sandhu Devinder , Kaundal Amita , Acharya Biswa R. , Forest Thomas , Pudussery Manju V. , Liu Xuan , Ferreira Jorge F. S. , Suarez Donald L.
Published: December 2020

Fourteen commercial almond rootstocks were tested under five types of irrigation waters to understand the genetic, physiological, and biochemical bases of salt-tolerance mechanisms. Treatments included control (T1) and four saline water treatments dominant in sodium-sulfate (T2), sodium-chloride (T3), sodium-chloride/sulfate (T4), and calcium/magnesium-chloride/sulfate (T5). T3 caused the highest reduction in survival rate and trunk diameter, followed by T4 and T2, indicating that Na and, to a lesser extent, Cl were the most toxic ions to almond rootstocks. Peach hybrid (Empyrean 1) and peach-almond hybrids (Cornerstone, Bright’s Hybrid 5, and BB 106) were the most tolerant to salinity. Rootstock’s performance under salinity correlated highly with its leaf Na and Cl concentrations, indicating that Na+and Cl-exclusion is crucial for salinity tolerance inPrunus. Photosynthetic rate correlated with trunk diameter and proline leaf ratio (T3/T1) significantly correlated with the exclusion of Na+and Cl-, which directly affected the survival rate. Expression analyses of 23 genes involved in salinity stress revealed that the expression differences among genotypes were closely associated with their performance under salinity. Our genetic, molecular, and biochemical analyses allowed us to characterize rootstocks based on component traits of the salt-tolerance mechanisms, which may facilitate the development of highly salt-tolerant rootstocks.
Assessment of the nutritional status of 6–36-month-old infants in Xinjiang and analysis of the influencing factors
Authors: Wei Meng , Li Jing , Yan Mei , Rena Maimaiti , Zhang Hui , Dong Ju
Published: December 2020

The health of infants is not only related to family happiness, but also to the future and development of the country. Therefore, it is still a very important public health problem to pay attention to the nutritional health level of infants. This article explores the nutritional health levels of infants and reveals the related risk factors. Stratified and multi-stage cluster sampling was used to select 3949 infants and young children in Xinjiang for the study. Survey staff conducted face-to-face questionnaire surveys to investigate their demographic characteristics, complementary food feeding, and related risk factors affecting their nutritional status. Study results showed that compared with the Han nationality, the Uygur and other nationalities were independent risk factors for malnutrition, as odds ratio (OR) values were 2.456 and 1.747, respectively (P < 0.05). When the feeders were not maternal, and their educational background was junior high school or below, OR values were 2.122 and 1.810, respectively (P <  0.05). The scores for non-breastfeeding and feeding behaviors were independent risk factors, and OR values were 1.983 and 2.709, respectively (P <  0.05). When infant minimum dietary diversity, minimum meal frequency, and minimum acceptable diet were unqualified, these indices were independent risk factors, and OR values were 2.281, 2.315, and 1.865, respectively (P <  0.05). The healthy growth of Han infants is better than that of other ethnic groups, which may be related to varying living environments, customs, social/economic development levels, educational levels, and other factors. In the future, the focus of our work should be to cooperate with the superior health organization to strengthen and improve the nutritional health level of infants.
Vestibular Infant Screening (VIS)–Flanders: results after 1.5 years of vestibular screening in hearing-impaired children
Authors: Martens Sarie , Dhooge Ingeborg , Dhondt Cleo , Vanaudenaerde Saartje , Sucaet Marieke , Rombaut Lotte , Boudewyns An , Desloovere Christian , Janssens de Varebeke Sebastien , Vinck Anne-Sophie , Vanspauwen Robby , Verschueren Dominique , Foulon Ina , Staelens Charlotte , Van den Broeck Karen , De Valck Claudia , Deggouj Naima , Lemkens Nele , Haverbeke Lisa , De Bock Mieke , Öz Okan , Declau Frank , Devroede Benoit , Verhoye Christoph , Maes Leen
Published: December 2020

Due to the close anatomical relationship between the auditory and vestibular end organs, hearing-impaired children have a higher risk for vestibular dysfunction, which can affect their (motor) development. Unfortunately, vestibular dysfunction often goes unnoticed, as vestibular assessment in these children is not standard of care nowadays. To timely detect vestibular dysfunction, the Vestibular Infant Screening–Flanders (VIS–Flanders) project has implemented a basic vestibular screening test for hearing-impaired infants in Flanders (Belgium) with a participation rate of 86.7% during the first year and a half. The cervical Vestibular Evoked Myogenic Potentials (cVEMP) test was applied as vestibular screening tool to map the occurrence of vestibular (mainly saccular) dysfunction in this population. At the age of 6 months, 184 infants were screened. No refers on vestibular screening were observed in infants with permanent conductive hearing loss. In infants with permanent sensorineural hearing loss, a cVEMP refer rate of 9.5% was observed. Failure was significantly more common in infants with severe-profound compared to those with mild-moderate sensorineural hearing loss (risk ratio = 9.8). Since this is the first regional study with a large sample size and successful participation rate, the VIS–Flanders project aims to set an example for other regions worldwide.
Repurposing of FDA approved ring systems through bi-directional target-ring system dual screening
Authors: Kumar Surendra , Jang Cheongyun , Subedi Lalita , Kim Sun Yeou , Kim Mi-hyun
Published: December 2020

In drug repurposing approaches, the chemically diverse and potentially safe molecules can be explored as therapeutic potential beyond those originally targeted indications. However, accessible information on a limited number of drug pipelines can lead to competitive over-heating issues, and intellectual property rights also restrict the free investigation in chemical space. As a complementary approach to the drawbacks, ring systems of approved drugs (instead of clinical drugs) can be optimized and used for repurposing purposes. In this study, bi-directional target (T) and ring system (R) dual screening (TR screening) was developed for the repurposing of their rarely used ring systems from FDA approved drugs. The TR screening suggested RAR β and cyproheptadine as the best pair of target and ring system to escape a saddle point. The selected ring system was virtually grown and elaborated with the defined criteria: synthesizability, drug-likeness, and docking pose showing the top scores. The achieved compounds were synthesized and biologically tested with an acceptable ADME/T profile.
Size distribution of virus laden droplets from expiratory ejecta of infected subjects
Authors: Anand S. , Mayya Y. S.
Published: December 2020

For rebooting economic activities in the ongoing COVID-19 pandemic scenario, it is important to pay detailed attention to infection transfer mechanisms during interaction of people in enclosed environments. Utmost concern is the possibility of aerosol mediated infection transfer, which is largely governed by the size distributions of virus laden droplets, termed as virusols in this work, ejected from humans. We expand on the well-known theory of Poisson fluctuations which acts as statistical barrier against formation of virusols. Analysis suggests that for viral loads < 2 × 105 RNA copies/mL, often corresponding to mild-to-moderate cases of COVID-19, droplets of diameter < 20 µm at the time of emission (equivalent to ~ 10 µm desiccated residue diameter) are unlikely to be of consequence in carrying infections. Cut-off diameters below which droplets will be practically free of contamination, are presented as a function of viral loading. The median diameters of virus laden polydisperse droplet distributions will be 1.5 to 20 times higher depending upon the geometric standard deviation. The studies have implications to risk assessment as well as residence time estimates of airborne infections in indoor environments. Additionally, it will be also helpful for performance evaluation of sanitization and control technologies to mitigate infection risks in workplaces.
The impact of Fe atom on the spin-filter and spin thermoelectric properties of Au-Fe@C20-Au monomer and dimer systems
Authors: Khalatbari H. , Vishkayi S. Izadi , Soleimani H. Rahimpour
Published: December 2020

Based on density functional theory and non-equilibrium Green’s function formalism, we explore the effect of Fe atom in Au-Fe@C20-Au monomer and dimer systems in comparison with the C20fullerene molecular junctions. We calculate the spin-dependent transmission coefficient, spin polarization and also their spin thermoelectric coefficients to investigate magnetic properties in the system. Our results indicate that the presence of Fe atoms enhances substantially the spin-filter and increases the spin figure of merit in the dimer system. We suggest that the Au-(Fe@C20)2-Au system is a suitable junction for designing spin-filtering and spin thermoelectric devices and eventually it is a good candidate for spintronic applications.