bluuebunny/crossref_metadata_2025 huggingface dataset:
The lipidome of Crithidia fasiculataand its plasticity
Authors: Cerone Michela , Roberts Matthew , Smith Terry K.
Published: October 2022

Crithidia fasiculata belongs to the trypanosomatidae order of protozoan parasites, bearing close relation to other kinetoplastid parasites such as Trypanosoma brucei and Leishmania spp. As an early diverging lineage of eukaryotes, the study of kinetoplastid parasites has provided unique insights into alternative mechanisms to traditional eukaryotic metabolic pathways. Crithidia are a monogenetic parasite for mosquito species and have two distinct lifecycle stages both taking place in the mosquito gut. These consist of a motile choanomastigote form and an immotile amastigote form morphologically similar to amastigotes in Leishmania. Owing to their close relation to Leishmania, Crithidia are a growing research tool, with continuing interest in its use as a model organism for kinetoplastid research with the added benefit that they are non-pathogenic to humans and can be grown with no special equipment or requirements for biological containment. Although comparatively little research has taken place on Crithidia, similarities to other kinetoplast species has been shown in terms of energy metabolism and genetics. Crithidia also show similarities to kinetoplastids in their production of the monosaccharide D-arabinopyranose similar to Leishmania, which is incorporated into a lipoarabinogalactan a major cell surface GPI-anchored molecule. Additionally, Crithidia have been used as a eukaryotic expression system to express proteins from other kinetoplastids and potentially other eukaryotes including human proteins allowing various co- and post-translational protein modifications to the recombinant proteins. Despite the obvious usefulness and potential of this organism very little is known about its lipid metabolism. Here we describe a detailed lipidomic analyses and demonstrate the possible placidity of Crithidia’s lipid metabolis. This could have important implications for biotechnology approaches and how other kinetoplastids interact with, and scavenge nutrients from their hosts.
Impact of shift work on surgical outcomes at different times in patients with acute type A aortic dissection: A retrospective cohort study
Authors: Zhang Xiang , Lv Weiwei , Liu Xin , Liu Kai , Yang Shaozhong
Published: October 2022

Background

To investigate the effect of shift work on surgical outcomes at different times in patients with acute type A aortic dissection (ATAAD).

Materials and methods

Patients with ATAAD who underwent total arch replacement at Qilu Hospital of Shandong University from January 2015 to March 2022 were retrospectively analyzed. All patients were managed according to the green channel emergency management strategy, and a professional cardiac team was arranged during off-hours. Based on surgery time and symptom onset to procedure time, the patients were divided into weekday, weekend and holiday groups; daytime and nighttime groups; intervention time ≤48 h and >48 h groups; working hours and off-hours groups. In-hospital mortality between these groups was compared.

Results

In total, 499 ATAAD patients underwent surgery within 7 days of symptom onset, and the in-hospital mortality rate was 10% (n = 50/499). Among the 499 patients, 320 (64.13%), 128 (25.65%) and 51 (10.22%) underwent surgery on weekdays, weekends and holidays, respectively. In-hospital mortality and 7-day mortality showed no significant difference among the three groups. Two hundred twenty-seven (45.5%) underwent daytime surgery, and 272 (54.5%) underwent nighttime surgery. Durations of ICU stay and hospital stay were significantly different between the two groups (P < 0.05). There was no significant differences in in-hospital mortality (9.2% vs. 10.7%) and 7-day mortality (4.4% vs. 6.6%). 221 patients (44.3%) and 278 patients (55.7%) were included in the intervention time ≤48 h and >48 h groups, respectively. Acute renal injury, ICU stay and hospital stay were significantly different (P < 0.05) whereas 7-day mortality (5.0% vs. 6.1%) and in-hospital mortality (8.6% vs. 11.1%) were not. Furthermore, 7-day (1.9% vs. 6.6%) and in-hospital mortality (11.1% vs. 9.8%) showed no difference between working hours group (n = 108) and off-hours group (n = 391). Cox regression analysis showed that postoperative acute renal injury (HR = 2.423; 95% CI, 1.214–4.834; P = 0.012), pneumonia (HR = 2.542; 95% CI, 1.186–5450; P = 0.016) and multiple organ dysfunction (HR = 11.200; 95% CI, 5.549–22.605; P = 0.001) were the main factors affecting hospital death in ATAAD patients.

Conclusion

Under the management of a professional cardiac surgery team with dedicated off-hours shifts, surgery time was not related to in-hospital mortality in ATAAD patients.
Role of the mitral valve in left ventricular assist device pathophysiology
Authors: Noly Pierre-Emmanuel , Duggal Neal , Jiang Mulan , Nordsletten David , Bonini Mia , Lei Ienglam , Ela Ashraf Abou El , Haft Jonathan W. , Pagani Francis D. , Cascino Thomas M. , Tang Paul C.
Published: October 2022

Functional mitral regurgitation (MR) in the setting of heart failure results from progressive dilatation of the left ventricle (LV) and mitral annulus. This leads to leaflet tethering with posterior displacement. Contrary to common assumptions, MR often does not resolve with LVAD decompression of the LV alone. The negative impact of significant (moderate-severe) mitral regurgitation in the LVAD setting is becoming better recognized in terms of its harmful effect on right heart function, pulmonary vascular resistance and hospital readmissions. However, controversies remain regarding the threshold for intervention and management. At present, there are no consensus indications for the repair of significant mitral regurgitation at the time of LVAD implantation due to the conflicting data regarding potential adverse effects of MR on clinical outcomes. In this review, we summarize the current understanding of MR pathophysiology in patients supported with LVAD and potential future management strategies.
Prediction of 30-day mortality in heart failure patients with hypoxic hepatitis: Development and external validation of an interpretable machine learning model
Authors: Sun Run , Wang Xue , Jiang Haiyan , Yan Yan , Dong Yansong , Yan Wenxiao , Luo Xinye , Miu Hua , Qi Lei , Huang Zhongwei
Published: October 2022

Background

This study aimed to explore the impact of hypoxic hepatitis (HH) on survival in heart failure (HF) patients and to develop an effective machine learning model to predict 30-day mortality risk in HF patients with HH.

Methods

In the Medical Information Mart for Intensive Care (MIMIC)-III and IV databases, clinical data and survival situations of HF patients admitted to the intensive care unit (ICU) were retrospectively collected. Propensity Score Matching (PSM) analysis was used to balance baseline differences between HF patients with and without HH. Kaplan Meier analysis and multivariate Cox analysis were used to determining the effect of HH on the survival of CF patients. For developing a model that can predict 30-day mortality in CF patients with HH, the feature recurrence elimination (RFE) method was applied to feature selection, and seven machine learning algorithms were employed to model construction. After training and hyper-parameter optimization (HPO) of the model through cross-validation in the training set, a performance comparison was performed through internal and external validation. To interpret the optimal model, Shapley Additive Explanations (SHAP) were used along with the Local Interpretable Model-agnostic Explanations (LIME) and the Partial Dependence Plot (PDP) techniques.

Results

The incidence of HH was 6.5% in HF patients in the MIMIC cohort. HF patients with HH had a 30-day mortality rate of 33% and a 1-year mortality rate of 51%, and HH was an independent risk factor for increased short-term and long-term mortality risk in HF patients. After RFE, 21 key features (21/56) were selected to build the model. Internal validation and external validation suggested that Categorical Boosting (Catboost) had a higher discriminatory capability than the other models (internal validation: AUC, 0.832; 95% CI, 0.819–0.845; external validation: AUC, 0.757 95% CI, 0.739–0.776), and the simplified Catboost model (S-Catboost) also had good performance in both internal validation and external validation (internal validation: AUC, 0.801; 95% CI, 0.787–0.813; external validation: AUC, 0.729, 95% CI, 0.711–0.745).

Conclusion

HH was associated with increased mortality in HF patients. Machine learning methods had good performance in identifying the 30-day mortality risk of HF with HH. With interpretability techniques, the transparency of machine learning models has been enhanced to facilitate user understanding of the prediction results.
The predictive value of pressure recording analytical method for the duration of mechanical ventilation in children undergoing cardiac surgery with an XGBoost-based machine learning model
Authors: Li Mingwei , Wang Shuangxing , Zhang Hui , Zhang Hongtao , Wu Yongjie , Meng Bing
Published: October 2022

Objective

Prolonged mechanical ventilation in children undergoing cardiac surgery is related to the decrease in cardiac output. The pressure recording analytical method (PRAM) is a minimally invasive system for continuous hemodynamic monitoring. To evaluate the postoperative prognosis, our study explored the predictive value of hemodynamic management for the duration of mechanical ventilation (DMV).

Methods

This retrospective study included 60 infants who underwent cardiac surgery. Cardiac index (CI), the maximal slope of systolic upstroke (dp/dtmax), and cardiac cycle efficiency (CCE) derived from PRAM were documented in each patient 0, 4, 8, and 12 h (T0, T1, T2, T3, and T4, respectively) after their admission to the intensive care unit (ICU). A linear mixed model was used to deal with the hemodynamic data. Correlation analysis, receiver operating characteristic (ROC), and a XGBoost machine learning model were used to find the key factors for prediction.

Results

Linear mixed model revealed time and group effect in CI and dp/dtmax. Prolonged DMV also have negative correlations with age, weight, CI at and dp/dtmax at T2. dp/dtmax outweighing CI was the strongest predictor (AUC of ROC: 0.978 vs. 0.811, p < 0.01). The machine learning model suggested that dp/dtmax at T2 ≤ 1.049 or < 1.049 in combination with CI at T0 ≤ 2.0 or >2.0 can predict whether prolonged DMV (AUC of ROC = 0.856).

Conclusion

Cardiac dysfunction is associated with a prolonged DMV with hemodynamic evidence. CI measured by PRAM immediately after ICU admission and dp/dtmax 8h later are two key factors in predicting prolonged DMV.
Case report: Transcatheter edge-to-edge repair after prior surgical tricuspid annuloplasty
Authors: Afzal Shazia , Haschemi Jafer , Bönner Florian , Kelm Malte , Horn Patrick
Published: October 2022

Residual and recurrent tricuspid regurgitation may occur frequently after surgical tricuspid valve repair. However, reoperation for tricuspid regurgitation is rare, although many patients are again highly symptomatic. Tricuspid transcatheter edge-to-edge repair (TEER) is a promising therapy for severe tricuspid regurgitation. Herein, we report a 77-year-old woman with recurrent symptomatic massive tricuspid regurgitation 2 years after sutured annuloplasty of the tricuspid valve. TEER was successfully performed using the TriClip® device and tricuspid regurgitation was reduced to a mild degree. In conclusion, tricuspid TEER is feasible following surgical suture annuloplasty. TEER is an alternative option for patients with a failed previous annuloplasty repair for tricuspid regurgitation to undergo a less invasive treatment rather than a potentially higher-risk reoperation.
Quantitative analysis of venous outflow with photo-plethysmography in patients with suspected thoracic outlet syndrome
Authors: Hersant Jeanne , Ramondou Pierre , Josse Charlotte , Lecoq Simon , Henni Samir , Abraham Pierre
Published: October 2022

Background

Venous compression is the second most frequent form of thoracic outlet syndrome (TOS). Although venous photo-plethysmography (PPG) has been largely used to estimate the consequences of chronic thromboses (Paget Schroetter syndrome), systematic direct quantitative recording of hemodynamic consequences of positional venous outflow impairment in patients with suspected TOS has never been reported.

Objective

We hypothesized that moving the arms forward (prayer: “Pra” position) while keeping the hands elevated after a surrender/candlestick position (Ca) would allow quantification of 100% upper limb venous emptying (PPGmax) and quantitative evaluation of the emptying observed at the end of the preceding abduction period (End-Ca-PPG), expressed in %PPGmax.

Materials and methods

We measured V-PPG in 424 patients referred for suspected TOS (age 40.9 years old, 68.3% females) and retrieved the results of ultrasound investigation at the venous level. We used receiver operating characteristics curves (ROC) to determine the optimal V-PPG values to be used to predict the presence of a venous compression on ultrasound imaging. Results are reported as a median (25/75 centiles). Statistical significance was based on a two-tailed p < 0.05.

Results

An End-Ca-PPG value of 87% PPGmax at the end of the “Ca” period is the optimal point to detect an ultrasound-confirmed positional venous compression (area under ROC: 0.589 ± 0.024; p < 0.001). This threshold results in 60.9% sensitivity, 47.6% specificity, 27.3% positive predictive value, 79.0% negative predictive value, and 50.8% overall accuracy.

Conclusion

V-PPG is not aimed at detecting the presence of a venous compression due to collateral veins potentially normalizing outflow despite subclavicular vein compression during abduction, but we believe that it could be used to strengthen the responsibility of venous compression in upper limb symptoms in TOS-suspected patients, with the possibility of non-invasive, bilateral, recordable measurements of forearm volume that become quantitative with the Ca-Pra maneuver.

Clinical trial registration

[ClinicalTrials.gov], identifier [NCT04376177].
Association between remnant cholesterol and heart failure: A prospective cohort study
Authors: Liu Heng , Zhang Jing , Li Zhangbin , Liu Jie , Lian Shuping , Le Jianhua
Published: October 2022

Background

Elevated remnant cholesterol (RC) is associated with a higher risk of various cardiac diseases. Heart failure (HF) usually occurs at the end stage of various cardiac diseases. However, there is limited research on the association between RC and the risk of HF. Therefore, we aimed to provide relevant evidence by determining whether a high RC level also influences the risk of HF.

Materials and methods

In this secondary analysis of the Atherosclerosis Risk in Communities (ARIC) study, we included 12,595 participants without coronary heart disease. We determined the association of the RC level as a continuous or categorical variable with the risk of HF using the multivariable-adjusted Cox proportional hazards models and restricted cubic spline curve.

Results

During a median follow-up of 22.5 years, 2,029 (16.1%) cases of HF occurred in all included participants. Compared with participants in the RC < 0.50 mmol/L group, the adjusted hazard ratio (HR) for HF increased progressively in participants with the RC level of 0.50 to 0.99 mmol/L, 1.00 to 1.49 mmol/L, and ≥1.50 mmol/L, from 1.17 (95% confidence interval [CI]: 1.05–1.30) to 1.27(95% CI: 1.08–1.49) and to 1.50 (95% CI: 1.14–1.97) (P for trend < 0.001). Cubic spline curves also revealed that the risk of HF increased with the RC level.

Conclusion

In the general population without coronary heart disease, a higher level of RC was significantly associated with a higher risk of HF, indicating that a higher RC level might be a potential risk factor for HF. Therefore, the management of blood cholesterol to reduce the risks of HF should focus not only on the traditional blood lipid parameters but also on the RC level.
Bioresorbable scaffolds vs. drug-eluting stents for patients with myocardial infarction: A systematic review and meta-analysis of randomized clinical trials
Authors: Liu Yong , Xiao Di , Wu Yang , Li Meng , Liu Jia , Zhuang Rui , Ma Liyong , Li Jingen , Zhang Lijing
Published: October 2022

Objective

To compare the efficacy and safety of bioresorbable scaffolds (BRS) with drug-eluting stents (DES) in patients with myocardial infarction undergoing percutaneous coronary interventions (PCI).

Methods

We performed a systematic review and meta-analysis of randomized controlled trials (RCTs) comparing BRS with DES on clinical outcomes with at least 12 months follow-up. Electronic databases of PubMed, CENTRAL, EMBASE, and Web of Science from inception to 1 March 2022 were systematically searched to identify relevant studies. The primary outcome of this study was the device-oriented composite endpoint (DOCE) consisting of cardiac death, target-vessel myocardial infarction, and target lesion revascularization. Secondary outcomes were a composite of major adverse cardiac events (MACE, all-cause death, target-vessel myocardial infarction, or target vessel revascularization) and the patient-oriented composite endpoint (POCE, defined as a composite of all-cause death, myocardial infarction, or revascularization). The safety outcomes were definite/probable device thrombosis and adverse events.

Results

Four randomized clinical trials including 803 participants with a mean age of 60.5 ± 10.8 years were included in this analysis. Patients treated with BRS had a higher risk of the DOCE (RR 1.62, 95% CI: 1.02–2.57, P = 0.04) and MACE (RR 1.77, 95% CI: 1.02–3.08, P = 0.04) compared with patients treated with DES. No significant difference on the POCE (RR 1.33, 95% CI: 0.89–1.98, P = 0.16) and the definite/probable device thrombosis (RR 1.31, 95% CI: 0.46–3.77, P = 0.61) were observed between BRS and DES. No treatment-related serious adverse events were reported.

Conclusion

BRS was associated with a higher risk of DOCE and MACE compared with DES in patients undergoing PCI for myocardial infarction. Although this seems less effective in preventing DOCE, BRS appears as safe as DES.

Systematic review registration

[https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=321501], identifier [CRD 42022321501].
Implications of new-onset atrial fibrillation on in-hospital and long-term prognosis of patients with acute myocardial infarction: A report from the CBD bank study
Authors: Yang Wang-Yang , Lip Gregory Y. H. , Sun Zhi-Jun , Peng Hui , Fawzy Ameenathul M. , Li Hong-Wei ,   The Cardiovascular Center Beijing Friendship Hospital Database Bank (CBD Bank) study group
Published: October 2022

Background

An increase in the incidence of atrial fibrillation (AF) during the acute phase of myocardial infarction (AMI) has been observed. But it is still unclear whether the implications of new-onset AF on in-hospital and long-term prognosis are of similar magnitude.

Methods

Using data from the CBD Bank study, 3,824 consecutive AMI patients, without prior AF, were analyzed. During the index hospitalization, all patients were monitored by continuous cardiac monitoring, twice daily performed 12- or 18-lead ECGs and timely ECG checks when cardiac symptoms occurred. Follow-up visits were routinely scheduled after discharge. Primary outcomes were all-cause death and cardiovascular death occurring during hospitalization and long-term follow-up. Secondary outcome was MACEs during hospitalization.

Results

During the median hospital stay of 9.0 (7.0, 11.0) days, new-onset AF was documented in 133 (3.48%) patients; 95 (71.43%) patients had AF attacks within 3 days following AMI. Independent risk factors associated with new-onset AF were older age, larger left atrial diameter, higher level of NT-proBNP, and primary PCI. New-onset AF was found to be significantly associated with in-hospital all-cause death (OR 4.33, 95%CI: 2.37-7.89, P < 0.001), cardiovascular death (OR 4.10, 95%CI: 2.18-7.73, P < 0.001), and MACEs (OR 2.51, 95%CI: 1.46-4.33, P = 0.001). A total of 112 new-onset AF and 3,338 non-AF patients were followed up for 1,090 (365, 1,694) days after discharge. There was no significant association between new-onset AF and long-term all-cause death (HR 1.21, 95%CI: 0.77-1.92, P = 0.406) or cardiovascular death (HR 1.09, 95%CI: 0.61-1.97, P = 0.764).

Conclusion

New-onset AF following AMI is strongly associated with an increased risk of adverse in-hospital prognosis, but it does not affect prognosis in those who survive until hospital discharge.
Time-of-day dependent effects of midazolam administration on myocardial injury in non-cardiac surgery
Authors: Prin Meghan , Pattee Jack , Douin David J. , Scott Benjamin K. , Ginde Adit A. , Eckle Tobias
Published: October 2022

Background

Animal studies have shown that midazolam can increase vulnerability to cardiac ischemia, potentially via circadian-mediated mechanisms. We hypothesized that perioperative midazolam administration is associated with an increased incidence of myocardial injury in patients undergoing non-cardiac surgery (MINS) and that circadian biology may underlie this relationship.

Methods

We analyzed intraoperative data from the Multicenter Perioperative Outcomes Group for the occurrence of MINS across 50 institutions from 2014 to 2019. The primary outcome was the occurrence of MINS. MINS was defined as having at least one troponin-I lab value ≥0.03 ng/ml from anesthesia start to 72 h after anesthesia end. To account for bias, propensity scores and inverse probability of treatment weighting were applied.

Results

A total of 1,773,118 cases were available for analysis. Of these subjects, 951,345 (53.7%) received midazolam perioperatively, and 16,404 (0.93%) met criteria for perioperative MINS. There was no association between perioperative midazolam administration and risk of MINS in the study population as a whole (odds ratio (OR) 0.98, confidence interval (CI) [0.94, 1.01]). However, we found a strong association between midazolam administration and risk of MINS when surgery occurred overnight (OR 3.52, CI [3.10, 4.00]) or when surgery occurred in ASA 1 or 2 patients (OR 1.25, CI [1.13, 1.39]).

Conclusion

Perioperative midazolam administration may not pose a significant risk for MINS occurrence. However, midazolam administration at night and in healthier patients could increase MINS, which warrants further clinical investigation with an emphasis on circadian biology.
The crosstalk among autophagy, apoptosis, and pyroptosis in cardiovascular disease
Authors: Cong Lin , Bai Yunpeng , Guo Zhigang
Published: October 2022

In recent years, the mechanism of cell death has become a hotspot in research on the pathogenesis and treatment of cardiovascular disease (CVD). Different cell death modes, including autophagy, apoptosis, and pyroptosis, are mosaic with each other and collaboratively regulate the process of CVD. This review summarizes the interaction and crosstalk of key pathways or proteins which play a critical role in the entire process of CVD and explores the specific mechanisms. Furthermore, this paper assesses the interrelationships among these three cell deaths and reviews how they regulate the pathogenesis of CVD. By understanding how these three cell death modes go together we can learn about the pathogenesis of CVD, which will enable us to identify new targets for preventing, controlling, and treating CVD. It will not only reduce mortality but also improve the quality of life.
Re-Live History: An immersive virtual reality learning experience of prehistoric intangible cultural heritage
Authors: Barbara Jonathan
Published: October 2022

The use of immersive virtual reality for learning is a growing opportunity that has so far suffered from limited application in the classroom, particularly with students in the 11 to 12 year bracket. Due to more concern being shown toward usability rather educational goals, mixed feelings exist about the technology’s ability to teach. Meanwhile, historical games usually have fun as the main or sole objective, which may cause problems by diminishing the value of the depicted cultural heritage and supersede the intended learning outcomes of the experience. This research aims to contribute toward this gap by working closely with teachers in developing an immersive virtual reality learning experience to teach prehistoric intangible cultural heritage to history students aged 11 to 12 years. The research question of this study is how to go about designing an immersive learning experience for secondary school teachers to teach 11 to 12 year old students about prehistoric cultural heritage on which very little documented evidence is available. To this end, the Re-Live History project was built upon a virtual reality navigation experience of a Maltese Neolithic hypogeum, adding a representation of intangible cultural heritage in the form of human behavior. A content requirement study from heritage experts’ perspective was carried out, followed by a similar study from the history teachers’ perspective. These provided which learning outcomes can be potentially addressed by the immersive learning experience, what form of intangible cultural heritage can be represented, and what success criteria were to be used for its evaluation. A prototype of the experience was then developed and reviewed by the heritage experts and subsequently developed into the experience evaluated by teachers and heads of department. Evaluation was carried out in terms of authenticity relative to the historic site, ease of navigation, impact in terms of achievable learning outcomes, and utility in the classroom. This ensured that educational objectives were given priority and should help teachers embrace and adopt the technology in the classroom. Future work should pilot the use of the IVR in the classroom and provide further empirical evidence to its ability to help such students achieve the learning outcomes expected by the syllabus.
The role of mothers in anti-corruption education: The development of “BUTIKO” as knowledge-sharing virtual community
Authors: Nugroho Sumarjiyanto B. M. , Susilowati Indah , Fachrunnisa Olivia , Prastyadewi Made Ika , Furoida Aini Nur
Published: October 2022

This article contends that if anti-corruption campaigns are to be effective, mothers must be involved in anti-corruption education, especially for children. The process of character-building will require early inculcation of anti-corruption ideals to prevent the manifestation of corrupt behavior in the future. However, lack of access to educational materials, especially in rural areas, makes the role of mothers in educating their children about corruption difficult. In order to overcome this impediment, this article advances a novel solution based on the use of information technology by mothers to offer anti-corruption education to their children in rural areas. The purpose of this article is to discuss the proposed framework of Bunda Anti Korupsi “Butiko” (BUTIKO) virtual community. The virtual community helps mothers (Bunda) and children to access character education and anti-corruption knowledge from experts. It is expected that the creation of the virtual community character education will help prevent future corruption.
Use of augmented reality in chromatography learning: How is this dynamic visual artifact fostering the visualization capacities of chemistry undergraduate students?
Authors: Merino Cristian , Marzábal Ainoa , Quiroz Waldo , Pino Sonia , López-Cortés Francisco , Carrasco Ximena , Miller Brant G.
Published: October 2022

Developing chemistry students' capability to use representations to explain phenomena is a challenging task for educators. To overcome chemistry students' learning difficulties, strategies that favor the development of visualization capabilities have been identified as productive. We are particularly interested in exploring the use of augmented reality in chemistry education to foster the development of those capabilities in undergraduate students. Our research objective was to analyze the contribution of augmented reality to support undergraduate chemistry students' visualization capacities while explaining the physical-kinetic processes of chromatography. Using an exploratory case study approach, we designed and conducted four task teaching and learning sequences, with seven Augmented Reality markers embedded. Thirty-eight undergraduate students, who voluntarily agreed to participate, explained the different elution rates of pigments in a chromatographic column. Their written accounts were analyzed to identify the level of sophistication of their representations. After using Augmented Reality, students' representations progressed from simple macroscopic descriptions of observed phenomena to explanations of processes where scientific ideas and microscopic representations were used as supporting evidence. Our study shows that the use of Augmented Reality has the potential to favor a more sophisticated use of representations when undergraduate students explain chromatographic processes. However, there are still limitations in reaching the highest levels of performance described in the literature.
Description of the molecular and phenotypic spectrum in Chinese patients with aggrecan deficiency: Novel ACAN heterozygous variants in eight Chinese children and a review of the literature
Authors: Deng Shuyun , Hou Lele , Xia Dan , Li Xiaojuan , Peng Xiaofang , Xiao Xiaoqin , Zhang Jieming , Meng Zhe , Zhang Lina , Ouyang Nengtai , Liang Liyang
Published: October 2022

Objective

This study analyzed eight Chinese short stature children with aggrecan deficiency, and aimed to investigate potential genotype–phenotype correlations, differences in clinical characteristics between the Chinese and the Western populations, and effectiveness of recombinant human growth hormone therapy in patients with ACAN variants through a review of the literature.

Methods

Pediatric short stature patients with ACAN heterozygous variants were identified using whole-exome sequencing. Subsequently, a literature review was carried out to summarize the clinical features, genetic findings, and efficacy of growth-promoting therapy in patients with ACAN variants.

Results

We identified seven novel ACAN mutations and one recurrent variant. Patients in our center manifested with short stature (average height SDS: -3.30 ± 0.85) with slight dysmorphic characteristics. The prevalence of dysmorphic features in the Chinese populations is significantly lower than that in the Western populations. Meanwhile, only 24.24% of aggrecan-deficient Chinese children showed significantly advanced bone age (BA). Promising therapeutic benefits were seen in the patients who received growth-promoting treatment, with an increase in growth velocity from 4.52 ± 1.00 cm/year to 8.03 ± 1.16 cm/year.

Conclusion

This study further expanded the variation spectrum of the ACAN gene and demonstrated that Chinese children with short stature who carried ACAN heterozygous variants exhibited early growth cessation, which may remain unnoticed by clinicians as most of these children had very mild dysmorphic characteristics and showed BA that was consistent with the chronological age. Genetic testing may help in the diagnosis.
Bisphenols A and F, but not S, induce apoptosis in bovine granulosa cells via the intrinsic mitochondrial pathway
Authors: Kourmaeva Emilia , Sabry Reem , Favetta Laura A.
Published: October 2022

With the gradual decline in global fertility rates, there is a need to identify potential contributing factors, their mechanisms of actions and investigate possible solutions to reverse the trend. Endocrine disrupting compounds (EDCs), such as bisphenol A (BPA), are environmental toxicants that are known to negatively impact reproductive functions. As such, the use of BPA in the manufacturing industry has slowly been replaced by analogs, including bisphenol S (BPS) and bisphenol F (BPF), despite limited knowledge available regarding their impact on health and their safety. The following study investigates the effects of BPA, BPS and BPF at a concentration of 0.5 μg/mL and 50 μg/mL on bovine granulosa cell apoptosis, with the ultimate goal of determining how they may impact oocyte competence and, thus, overall fertility. The underlying hypothesis is that bisphenols disrupt the granulosa cell environment surrounding the oocyte inducing excessive apoptosis via the intrinsic mitochondrial pathway. To test this hypothesis, apoptosis was measured following a time- and dose-dependent exposure to all three bisphenols by flowcytometry paired with annexin V/PI staining as well as by quantification of key genes belonging to the intrinsic apoptotic pathway both at the mRNA and protein levels. The results of this study report that BPA and BPF reduce cell viability through reduced cell counts and increased apoptosis. This increase is due, in part, to the induction of apoptotic genes of the intrinsic pathway of apoptosis. Additionally, this study also suggests that BPS may not act on the intrinsic mitochondrial apoptotic pathway in bovine granulosa cells. Overall, this study allows us to establish potential apoptotic pathways activated by bisphenols as well as compare the relative apoptotic activities of BPA to its most widespread analogs.
Effect of Lactobacillus casei fermented milk on fracture healing in osteoporotic mice
Authors: Guo Xing , Zhong Kai , Zou LongFei , Xue Hao , Zheng ShuLing , Guo Jiang , Lv Hui , Duan Ke , Huang DengHua , Tan MeiYun
Published: October 2022

The interaction between the gut microbiota and the host has been described experimentally by germ-free animals or by antibiotic-disturbed gut microbiota. Studies on germ-free mice have shown that gut microbiota is critical for bone growth and development in mice, emphasizing that microbiota dysbiosis may interfere with normal bone development processes. This study aimed to clarify the effect of antibiotic treatment on disturbed gut microbiota on bone development in mice and to investigate the effect of probiotic treatment on fracture healing in mice with dysbiosis. Our results showed that 4 weeks old female Kunming mice showed significantly lower abundance and diversity of the gut microbiota and significantly lower bone mineral density after 12 weeks of antibiotic treatment and significantly increased levels of RANKL and Ang II in serum (p<0.05). Mice with dysbiosis received 5 mL of Lactobacillus casei fermented milk by daily gavage after internal fixation of femoral fractures, and postoperative fracture healing was evaluated by X-ray, micro-CT scan, and HE staining, which showed faster growth of the broken ends of the femur and the presence of more callus. Serological tests showed decreased levels of RANKL and Ang II (p<0.05). Similarly, immunohistochemical results also showed increased expression of α smooth muscle actin in callus tissue. These results suggest that oral antibiotics can lead to dysbiosis of the gut microbiota in mice, which in turn leads to the development of osteoporosis. In contrast, probiotic treatment promoted fracture healing in osteoporotic mice after dysbiosis, and the probiotic effect on fracture healing may be produced by inhibiting the RAS/RANKL/RANK pathway.
Endometrial thickness is associated with low birthweight in frozen embryo transfer cycles: A retrospective cohort study of 8,235 singleton newborns
Authors: He Tingting , Li Mingzhao , Li Wei , Meng Peng , Xue Xia , Shi Juanzi
Published: October 2022

Objective

To explore the association between endometrial thickness (EMT) and adverse neonatal outcomes in frozen in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) cycles.

Methods

This retrospective study involved a total of 8,235 women under the age of 35 years who underwent IVF/ICSI cycles and received frozen embryo transfer (FET) at a tertiary-care academic medical from January 2015 to December 2019, resulting in a live singleton newborn. Patients were categorized into three groups depending on EMT: ≤7.5 mm, 7.5-12 mm and >12 mm. The primary outcome was low birthweight (LBW). The secondary outcomes were preterm birth (PTB), small-for-gestational age (SGA), large-for-gestational age (LGA) and high birthweight (HBW).

Result(s)

Compared with EMT >7.5–12 mm group, the risk of being born LBW was statistically significantly increased in the EMT ≤7.5 mm group (adjusted odds ratio [aOR] 2.179; 95% confidence interval [CI], 1.305–3.640; P=.003), while dramatically decreased in the EMT >12 mm group (aOR 0.584; 95% CI, 0.403-0.844; P=.004). Moreover, newborn gender and pregnancy complications were all independent predictors for LBW. Furthermore, a significant decrease in birthweight was found in the EMT ≤7.5 mm group as compared with EMT >7.5–12 mm group and EMT >12 mm group (3,239 ± 612 vs. 3,357 ± 512 and 3,374 ± 479 g, respectively), and similar result was found in term of gestational age (38.41 ± 2.19 vs. 39.01 ± 1.68 and 39.09 ± 1.5 weeks, respectively).

Conclusion(s)

After frozen IVF/ICSI-ET, EMT ≤7.5 mm is independently associated with increased risk of LBW among women with singleton newborns. Therefore, we suggest that women with EMT ≤7.5 mm after achieving pregnancy by IVF/ICSI-ET treatment should warrant more attention to reduce the risk of delivering a LBW newborn.
Dynamic Nexus between macroeconomic factors and CO2 emissions: Evidence from oil-producing countries
Authors: Gazi Md. Abu Issa , Nahiduzzaman Md. , Shaturaev Jakhongir , Dhar Bablu Kumar , Halim Md. Abdul
Published: October 2022

Current literature conveys that in spite of multiple studies being conducted to explore the influences of various macroeconomic factors both geographical and non-geographical on the CO2 emissions in different parts of the world, there is a scarcity of the same analyses from oil-producing countries. In this study, we reveal a new dimension by investigating the dynamic linkage of climate change, economic growth, energy use, and agricultural and rural development to the CO2 emissions of oil-producing countries around the world. In doing so, we apply Pedroni and Kao panel cointegration test, vector error correction model (VECM), pairwise Granger causality test, impulse response function (IRF), and some supportive models such as-generalized method of moments (GMM), and fixed-effect models. Our primary VAR-based models’ evidence that energy use (EUE), foreign direct investment (FDI), and trade to GDP (TPR) rate have both short-run and long-run casual consequences in CO2 emissions, while only long-run Granger causality is running from agricultural land ratio (ALR), forest area ratio (FAR), gross domestic product (GDP), population growth rate (PGR), renewable energy consumption (REC), and rural population rate (RPR) to CO2 emissions. However, bidirectional associations are observed between CO2 to foreign direct investment and trade percentage rate; EUE to renewable energy consumption and TPR; and TPR to FDI and gross domestic product. To demonstrate the significant impact, our secondary analysis tools GMM and fixed-effect regressions’ results disclose that high energy use and more domestic products significantly contaminate the environmental condition by increasing CO2 emissions in the atmosphere. Hence, our research provides great implications for the authorities of government, producers, businessmen, and general public in the oil-producing countries to ensure a sustainable environment by reducing energy use or alternating with renewable energies and emphasizing environmentally friendly products production over the long-run rather than conventional products production in the short-run.
Construction of influencing factor model for high-quality green development of Chinese industrial enterprises
Authors: Fang Zhen , Yang Can , Song Xiaowei
Published: October 2022

The strategic planning and decision making of the government in the combination of high-quality development and green development has had a far-reaching impact on the development of industrial enterprises. This paper grounded theory to build a theoretical framework model of the factors that influence the development of high-quality green industrial enterprises. The data are based on policy documents, policy interpretations, official public opinion, and other original text materials related to the high-quality green development of Chinese industrial enterprises. The research finds that the factors that influence the high-quality green development of industrial enterprises can be divided into three dimensions, including the environmental dimension, the enterprise dimension, and the process dimension. Among them, the enterprise dimension is the most core dimension and directly affects the high-quality green development of industrial enterprises. Different parts (six parts) in each dimension are connected together to form a circular chain of high-quality green development of industrial enterprises. There is also an interaction mechanism within each part.
Incorporating conceptual site models into national-scale environmental risk assessments for legacy waste in the coastal zone
Authors: Riley Alex L. , Amezaga Jaime , Burke Ian T. , Byrne Patrick , Cooper Nick , Crane Richard A. , Comber Sean D.W. , Gandy Catherine J. , Hudson-Edwards Karen A. , Jennings Elin , Lewis Elizabeth , Lofts Stephen , MacDonald John M. , Malcolm Heath , Mayes William M. , Onnis Patrizia , Olszewska Justyna , Spears Bryan , Jarvis Adam P.
Published: October 2022

Solid wastes deposited in the coastal zone that date from an era of lax environmental regulations continue to pose significant challenges for regulators and coastal managers worldwide. The increasing risk of contaminant release from these legacy disposal sites, due to a range of factors including rising sea levels, associated saline intrusion, and greater hydrological extremes, have been highlighted by many researchers. Given this widespread challenge, and the often-limited remedial funds available, there is a pressing need for the development of new advanced site prioritization protocols to limit potential pollution risks to sensitive ecological or human receptors. This paper presents a multi-criteria decision analysis that integrates the principles of Conceptual Site Models (Source-Pathway-Receptor) at a national scale in England and Wales to identify legacy waste sites where occurrence of pollutant linkages are most likely. A suite of spatial data has been integrated in order to score potential risks associated with waste type (Source), likelihood of pollutant release relating to current and future flood and erosion climate projections, alongside current management infrastructure (Pathway), and proximity to sensitive ecological features or proxies of human use in coastal areas (Receptors). Of the 30,281 legacy waste deposits identified in England and Wales, 3,219 were located within the coastal zone, with coastal areas containing a density of legacy wastes (by area) 10.5 times higher than inland areas. Of these, 669 were identified as priority sites in locations without existing coastal defences or flood management infrastructure, with 2550 sites identified in protected areas where contaminant transfer risks could still be apparent. The majority (63%) of the priority sites have either undefined source terms, or are classified as mixed wastes. Mining and industrial wastes were also notable waste categories, and displayed strong regional distributions in the former mining areas of north-east and south-west of England, south Wales, and post-industrial estuaries. The large-scale screening process presented here could be used by environmental managers as a foundation to direct more high-resolution site assessment and remedial work at priority sites, and can be used as a tool by governments for directing funding to problematic sites. List of Acronyms.
Thermokarst lake changes over the past 40 years in the Qinghai–Tibet Plateau, China
Authors: Li Lan , Zhang Xindi , Li Xuan , Zhao Shufang , Ni Wankui , Yang Zhenzhen
Published: October 2022

A thermokarst lake is generally defined as a lake that occupies a closed basin and is created by the massive melting of ground ice in ice-rich permafrost regions, which has a great impact on regional hydro-ecological equilibrium and permafrost-engineering infrastructure facilities. Global warming and increasing human activities have been accompanied by permafrost degradation and glacier retreat in the Qinghai–Tibet Plateau (QTP). The QTP, source of many international rivers in Asia, is known as the “Asian Water Tower.” The number and area of lakes in the QTP have increased in the past 40 years; however, lakes with areas of less than 1 km2 have been overlooked when calculating the water storage. To address the evolution of thermokarst lakes in the QTP, visual interpretation and the Modified Normalized Difference Water Index were applied to extract the water area based on Landsat data from the 1980s to 2020. The results indicate that thermokarst lake area was reduced from 932.5 km2 to 799.25 km2 from the 1980s to 1990. From 1990 to 2020, the number and area of lakes grew substantially, with the number increasing from 66506 to 120374 and the area increasing by 113.14% (932.5 km2 in 1980s and 1703.56 km2 in 2020). As heat carriers, thermokarst lakes transfer heat vertically and horizontally. The temperature of the surrounding permafrost rises as the number and area of lakes increase and when permafrost is extensively degraded. Longtime carbon stocks are also released into atmosphere during lake formation, which affects the regional carbon cycle.
Genomic approaches for monitoring transmission dynamics of malaria: A case for malaria molecular surveillance in Sub–Saharan Africa
Authors: Mensah Benedicta A. , Akyea-Bobi Nukunu E. , Ghansah Anita
Published: October 2022

Transmission dynamics is an important indicator for malaria control and elimination. As we move closer to eliminating malaria in Sub-Saharan Africa (sSA), transmission indices with higher resolution (genomic approaches) will complement our current measurements of transmission. Most of the present programmatic knowledge of malaria transmission patterns are derived from assessments of epidemiologic and clinical data, such as case counts, parasitological estimates of parasite prevalence, and Entomological Inoculation Rates (EIR). However, to eliminate malaria from endemic areas, we need to track changes in the parasite population and how they will impact transmission. This is made possible through the evolving field of genomics and genetics, as well as the development of tools for more in-depth studies on the diversity of parasites and the complexity of infections, among other topics. If malaria elimination is to be achieved globally, country-specific elimination activities should be supported by parasite genomic data from regularly collected blood samples for diagnosis, surveillance and possibly from other programmatic interventions. This presents a unique opportunity to track the spread of malaria parasites and shed additional light on intervention efficacy. In this review, various genetic techniques are highlighted along with their significance for an enhanced understanding of transmission patterns in distinct topological settings throughout Sub-Saharan Africa. The importance of these methods and their limitations in malaria surveillance to guide control and elimination strategies, are explored.