bluuebunny/crossref_metadata_2025 huggingface dataset:
Forest Resource Quality and Human Activity Intensity Change and Spatial Autocorrelation Analysis in Yulin City, China
Authors: Song Chao , Yu Qiyin , Jin Kun
Published: September 2023

With the rapid development of society and the economy, human activities are increasing, which often brings potential threats such as a decline in forest resource quality and ecological function. In order to investigate the change in forest resource quality and human activity intensity, this study constructed a calculation model for a forest resource quality index and a human activity intensity index and conducted a quantitative analysis of the temporal and spatial changes in forest resource quality and human activity intensity in Yulin City based on sub-compartment data in 2017 and 2020. By combining spatial autocorrelation analysis, the changes in human activity intensity and spatial forest resource quality were explored, and key areas such as the prominent contradictions between humans and the land were superimposed and coupled as potential areas of concern. The results show the following: From 2017 to 2020, the forest resource quality in Yulin City improved as a whole, especially in Zizhou County, but there were increases and decreases in other regions. Human activity intensity increased as a whole, and the most obvious increase was in Hengshan District. Both the forest resource quality and human activity intensity indexes had spatial aggregation, the differences in forest resource quality between regions were reduced, and human activity intensity showed a trend towards aggregated development. The high–high cluster area for human activity intensity showed a decreasing trend, but it expanded outward in urban areas and other human-gathering areas, such as the surrounding area of Yulin City, Jingbian County, and Shenmu City. The high–high cluster area for forest resource quality showed a shrinking trend. Four specific regions were identified through a spatial coupled superposition analysis to reveal the dynamic relationship between forest resource quality and human activity intensity. The most obvious region was the Yuyang District, where the forest resource quality improved because of a reduction in the pressure of human activities on the natural environment.
Using Social Media and Multi-Source Geospatial Data for Quantifying and Understanding Visitor’s Preferences in Rural Forest Scenes: A Case Study from Nanjing
Authors: Wang Chongxiao , Zou Jiahui , Fang Xinyuan , Chen Shuolei , Wang Hao
Published: September 2023

Rapid urbanization has made urban forest scenes scarce resources, leading to a surge in the demand for high-quality rural forest scenes as alternative outdoor recreation spaces. Previous studies mainly applied survey methods, focusing on visitors’ feedback for different types of scenes from the perspective of visual quality evaluation. Nevertheless, the explanations of the relationships between various factors of scenes and visitors’ preferences are relatively superficial. This study sought to explore the distribution and characteristics of preferred rural forest scenes based on visitor reviews from social media, and using Geodetector, a geospatial statistics tool, to quantitatively analyzed the reasons for visitors’ preferences in terms of factors obtained from multi-source geospatial data. The findings are that (1) visitors are already satisfied with the natural environment but expect scenes that reflect the culture of tea; (2) spatial factor has a more robust interpretation of visitors’ preference, and although the Normalized Difference Vegetation Index (NDVI) and non-consumption indicators barely explain visitors’ preference solely when each of them is combined with other indicators, they can produce non-linear enhancement effects. Consequently, this study synthesizes visitors’ feedback and factors in rural forest scenes to understand visitors’ preferences, thus providing insights into human-centered planning.
In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate
Authors: Zhang Yang , Cai Bingqi , Liu Meng , He Keqin , Gong Zhijin , Bi Haoran , Wang Kai , Chen Biqiang , Wang Meng , Su Haijia , Tan Tianwei
Published: September 2023

The green biosynthesis of adipic acid, an important monomer of nylon 6,6, has become a research hotspot. α-Aminoadipate is a key intermediate in the metabolic pathway that converts L-lysine to produce adipic acid. In addition, metabolic flux analysis has become an important part of metabolic engineering. Many metabolic optimization algorithms have been developed to predict engineering intervention strategies with the aim of improving the production of target chemicals. Here, OptHandle, a new metabolic optimization algorithm, has been developed. And, we use OptHandle to optimize the biosynthesis of α-aminoadipate. Based on the results of OptHandle, an engineered Escherichia coli with a 13-fold higher titer was obtained, and 1.10 ± 0.02 g/L of α-aminoadipate was produced. The efficient synthesis of α-aminoadipate lays a foundation for the green production of adipic acid.
High Endemism in an Endangered Biodiversity Hotspot: Phylogeny, Taxonomy and Distribution Patterns of Catfishes of the Psammocambeva Alpha-Clade (Siluriformes: Trichomycteridae) from the Rio Doce Basin, Brazil
Authors: Costa Wilson J. E. M. , Mattos José Leonardo O. , Barbosa Maria Anaïs , Vilardo Paulo J. , Katz Axel M.
Published: September 2023

The Rio Doce basin is situated in a biodiversity hotspot, with some fish groups still superficially known. The intense process of habitat loss and water contamination justifies efforts to speed up the pace of describing fish species diversity. Herein, we focus on a clade of trichomycterine catfishes, subgenus Psammocambeva, genus Trichomycterus, performing a multigene phylogenetic analysis, which integrated to a comparative morphological analysis corroborates monophyly and relationships of four species complexes. Five new species are recognised and described. Species distribution patterns support the recognition of five areas of endemism for trichomycterines, most of which are well-known biodiversity centres: Serra do Espinhaço, Serra do Brigadeiro, Serra do Caparaó, Serra do Castelo, and upper-middle Rio Doce. Trichomycterine diversity in the Rio Doce basin, with 16 species, of which 15 belong to Psammocambeva, is lower than in the smaller neighbouring Rio Paraíba do Sul basin, with 21 species in five subgenera. Data suggest that species diversification in the former basin is relatively younger, following the initial diversification of Psammocambeva in the Miocene, whereas diversification in the latter basin has been estimated to have occurred during the Oligocene. This study reinforces the importance of naming new taxa and delimitating areas of endemism as primary tools for conservation strategies.
Collaborative Study for Iodine Monitoring in Mandatory Direct-Iodized Sauce in Thailand
Authors: Photi Juntima , Judprasong Kunchit , Gowachirapant Sueppong , Srisakda Premmin , Supanuwat Jutharat , Zeder Christophe
Published: September 2023

Direct iodization in fish sauce, soy sauce, and seasoning sauces plays a crucial role in optimizing the iodine intake of Thailand’s people. However, determining the iodine content to ensure that these sauces meet the standard of Thailand’s Food and Drug Administration (FDA) is challenging. In this study, all local laboratories equipped with inductively coupled plasma–mass spectrometry (ICP-MS) and with experience in iodine analysis by any analytical method were invited to participate in a hands-on training workshop and two rounds of interlaboratory comparison. The aim was to improve laboratory performance and assess the potential for iodine monitoring for mandatory direct-iodized sauces. All target laboratories participated in this study. The hands-on training workshop harmonized the analytical method and increased the capacity of participating laboratories. Most laboratories (7/8) achieved satisfactory performance for six test samples based on interlaboratory comparison. Samples were extracted by tetramethylammonium hydroxide (TMAH), with the presence of 6% 2-propanol, 0.01% triton X-100, internal standard, and iodine determination in direct-iodized sauces by ICP-MS. The reproducibility standard deviation (SL), after the removal of outlier results for iodine content, was 7–22% iodine at a level of 0.03–4.81 mg/L. Moreover, the Thai FDA’s judgment range for official control activities should expand the range of 2–3 mg per 1 L (ppm) by at least 22%.
Bacteriocin-Producing Enterococcus faecium OV3-6 as a Bio-Preservative Agent to Produce Fermented Houttuynia cordata Thunb. Beverages: A Preliminary Study
Authors: Choeisoongnern Thiwanya , Chaiyasut Chaiyavat , Sivamaruthi Bhagavathi , Makhamrueang Netnapa , Peerajan Sartjin , Sirilun Sasithorn , Sittiprapaporn Phakkharawat
Published: September 2023

Microbial contamination affects the quality of the fermented Houttuynia cordata Thunb. (H. cordata) beverage (FHB). The present study aimed to assess the bio-preservative property of Enterococcus faecium OV3-6 (E. faecium OV3-6) during the production of FHB. The antimicrobial activity against Escherichia coli, Salmonella, Bacillus cereus, and Staphylococcus aureus and the survival of E. faecium OV3-6 were studied. Then, FHB fermentation was performed with different preservatives (non-preservative, E. faecium OV3-6, cell-free supernatant of E. faecium OV3-6, and nisin) with and without representative pathogens. The maximum antimicrobial activity against S. aureus and B. cereus was observed after 18 h of cultivation in an MRS medium. E. faecium OV3-6 was used as a starter to produce the FHB, and the strain survived up to 48 h in the fermented beverage. E. faecium OV3-6 and its cell-free supernatant inhibited the growth of E. coli, Salmonella, B. cereus, and S. aureus in the stimulated FHB. The non-preservatives and nisin-containing FHB showed inhibition against Gram-positive pathogens. The FHB treated with E. faecium OV3-6 was rich in lactic acid bacteria, and the product was at an acceptable level of pH (less than 4.3). Certain limitations were identified in the study, such as lack of nutritional, metabolomics analysis, and safety and consumer acceptability of FHB. The results suggested that E. faecium OV3-6 could be used as a bio-preservative to produce fermented plant beverages (FPBs).
Incidents and Potential Adverse Health Effects of Serious Food Fraud Cases Originated in Asia
Authors: Kemsawasd Varongsiri , Jayasena Vijay , Karnpanit Weeraya
Published: September 2023

Food fraud has long been regarded as a major issue within the food industry and is associated with serious economic and public health concerns. Economically motivated adulteration, the most common form of food fraud, has consequences for human health, ranging from mild to life-threatening conditions. Despite the potential harm and public health threats posed by food fraud, limited information on incidents causing illness has been reported. Enhancing the food control system on the Asian continent has become crucial for global health and trade considerations. Food fraud databases serve as valuable tools, assisting both the food industry and regulatory bodies in mitigating the vulnerabilities associated with fraudulent practices. However, the availability of accessible food fraud databases for Asian countries has been restricted. This review highlights detrimental food fraud cases originating in Asian countries, including sibutramine in dietary supplements, plasticizer contamination, gutter oil, and the adulteration of milk. This comprehensive analysis encompasses various facets, such as incident occurrences, adverse health effects, regulatory frameworks, and mitigation strategies.
The Rheology and Textural Properties of Bakery Products Upcycling Brewers’ Spent Grain
Authors: Ramu Ganesan Abirami , Hoellrigl Philipp , Mayr Hannah , Martini Loesch Demian , Tocci Noemi , Venir Elena , Conterno Lorenza
Published: September 2023

This study aimed to evaluate the rheological properties of doughs with 50% brewers’ spent grain (BSG) derived from a rye-based (RBSG) and barley-based (BBSG) beer added, and the textural profile of the related baked products. Simple model systems using BSG flour mixed with water were studied. Two bakery products, focaccia and cookies, were made as food systems using BSG in a 1:1 ratio with wheat flour (WF). Their rheological properties and texture after baking were characterized. BSG-added dough exhibited viscoelastic properties with a solid gel-like behavior. The addition of BSG increased G′ > G″ and decreased the dough flexibility. BSG addition in baked RBSG focaccia increased the hardness, gumminess, and chewiness by 10%, 9%, and 12%, respectively. BBSG cookies had a 20% increase in fracturability. A positive correlation was found between the rheological metrics of the dough and the textural parameters of BBSG-added cookies. PCA analysis revealed that complex viscosity, G′, G″, and cohesiveness separated BBSG focaccia from RBSG focaccia and the control. Therefore, the rheological properties of BSG dough will have industrial relevance for 3D-printed customized food products with fiber. Adding RBSG and BBSG to selected foods will increase the up-cycling potential by combining techno-functional properties.
Using Science and Technology to Unveil The Hidden Delicacy Terfezia arenaria, a Desert Truffle
Authors: Ferreira Inês , Dias Teresa , Mouazen Abdul M. , Cruz Cristina
Published: September 2023

Terfezia arenaria is a desert truffle native to the Mediterranean Basin region, highly appreciated for its nutritional and aromatic properties. Despite the increasing interest in this desert truffle, T. arenaria is not listed as an edible truffle authorized for trade in the European Union. Therefore, our objective was to showcase T. arenaria’s nutritional and chemical composition and volatile profile. The nutritional analysis showed that T. arenaria is a good source of carbohydrates (67%), proteins (14%), and dietary fibre (10%), resulting in a Nutri-Score A. The truffle’s volatile profile was dominated by eight-carbon volatile compounds, with 1-octen-3-ol being the most abundant (64%), and 29 compounds were reported for the first time for T. arenaria. T. arenaria’s nutritional and chemical compositions were similar to those of four commercial mushroom and truffle species, while the aromatic profile was not. An electronic nose corroborated that T. arenaria‘s aromatic profile differs from that of the other four tested mushroom and truffle species. Our data showed that T. arenaria is a valuable food resource with a unique aroma and an analogous composition to meat, which makes it an ideal source for plant-based meat products. Our findings could help promote a sustainable future exploitation of T. arenaria and ensure the quality and authenticity of this delicacy.
On Exotic Objects Made of Dark Energy and Dark Matter: Mass-to-Radius Profiles and Tidal Love Numbers
Authors: Sepúlveda Camila , Panotopoulos Grigoris
Published: September 2023

We investigate some properties of exotic spherical configurations made of dark matter and dark energy. For the former, we adopt a polytropic equation-of-state, while for the latter, we adopt the extended Chaplygin gas equation-of-state. Solving the Tolman–Oppenheimer–Volkoff equations, within the two-fluid formalism, we compute the factor of compactness, the mass-to-radius relationships, as well as the tidal Love numbers and dimensionless deformabilities. A comparison between single-fluid objects and two-fluid configurations is made as well.
Identification and Characterization of ABCG15—A Gene Required for Exocarp Color Differentiation in Pear
Authors: Zhang Simeng , Xu Jiayu , Zhang Ying , Cao Yufen
Published: September 2023

Exocarp color is a commercially essential quality for pear which can be divided into two types: green and russet. The occurrence of russet color is associated with deficiencies and defects in the cuticular and epidermal layers, which affect the structure of the cell wall and the deposition of suberin. Until now, the genetic basics triggering this trait have not been well understood, and limited genes have been identified for the trait. To figure out the gene controlling the trait of exocarp color, we perform a comprehensive genome-wide association study, and we describe the candidate genes. One gene encoding the ABCG protein has been verified to be associated with the trait, using an integrative analysis of the metabolomic and transcriptomic data. This review covers a variety of omics resources, which provide a valuable resource for identifying gene-controlled traits of interest. The findings in this study help to elucidate the genetic components responsible for the trait of exocarp color in pear, and the implications of these findings for future pear breeding are evaluated.
Aromatic L-Amino-Acid Decarboxylase Deficiency Screening by Analysis of 3-O-Methyldopa in Dried Blood Spots: Results of a Multicentric Study in Neurodevelopmental Disorders
Authors: Rizzi Susanna , Spagnoli Carlotta , Bellini Melissa , Cesaroni Carlo Alberto , Spezia Elisabetta , Bergonzini Patrizia , Caramaschi Elisa , Soliani Luca , Turco Emanuela Claudia , Piccolo Benedetta , Demuth Laura , Cordelli Duccio Maria , Biasucci Giacomo , Frattini Daniele , Fusco Carlo
Published: September 2023

Aromatic L-amino acid decarboxylase deficiency (AADCd) is a rare recessive metabolic disorder caused by pathogenic homozygous or compound heterozygous variants in the dopa decarboxylase (DDC) gene. Adeno-associated viral vector-mediated gene transfer of the human DDC gene injected into the putamen is available. The typical presentation is characterized by early-onset hypotonia, severe developmental delay, movement disorders, and dysautonomia. Recently, mild and even atypical phenotypes have been reported, increasing the diagnostic challenge. The aim of this multicentric study is to identify the prevalence of AADCd in a population of patients with phenotypic clusters characterized by neurodevelopmental disorders (developmental delay/intellectual disability, and/or autism) by 3-O-methyldopa (3-OMD) detection in dried blood spots (DBS). It is essential to identify AADCd promptly, especially within non-typical phenotypic clusters, because better results are obtained when therapy is quickly started in mild-moderate phenotypes. Between 2021 and 2023, 390 patients with non-specific phenotypes possibly associated with AADCd were tested; none resulted in a positive result. This result highlights that the population to be investigated for AADCd should have more defined clinical characteristics: association with common signs (hypotonia) and/or pathognomonic symptoms (oculogyric crisis and dysautonomia). It is necessary to continue to screen selected clusters for reaching diagnosis and improving long-term outcomes through treatment initiation. This underscores the role of newborn screening in identifying AADCd.
CeRNA Network Reveals the Circular RNA Characterization in Goat Ear Fibroblasts Reprogramming into Mammary Epithelial Cells
Authors: Sahito Jam Zaheer Ahmed , Deng Shan , Qin Liangshan , Xiao Lianggui , Zhang Dandan , Huang Ben
Published: September 2023

Circular RNAs (circRNAs) are a type of non-coding RNA that play a crucial role in the development and lactation of mammary glands in mammals. A total of 107 differentially expressed circRNAs (DE circRNAs) were found, of which 52 were up-regulated and 55 were down-regulated. We also found that DE circRNA host genes were mainly involved in GO terms related to the development process of mammary epithelial cells and KEGG pathways were mostly related to mammary epithelial cells, lactation, and gland development. Protein network analysis found that DE circRNAs can competitively bind to miRNAs as key circRNAs by constructing a circRNA–miRNA–mRNA network. CircRNAs competitively bind to miRNAs (miR-10b-3p, miR-671-5p, chi-miR-200c, chi-miR-378-3p, and chi-miR-30e-5p) involved in goat mammary gland development, mammary epithelial cells, and lactation, affecting the expression of core genes (CDH2, MAPK1, ITGB1, CAMSAP2, and MAPKAPK5). Here, we generated CiMECs and systematically explored the differences in the transcription profile for the first time using whole-transcriptome sequencing. We also analyzed the interaction among mRNA, miRNA, and cirRNA and predicted that circRNA plays an important role in the maintenance of mammary epithelial cells.
Independent COL17A1 Variants in Cats with Junctional Epidermolysis Bullosa
Authors: Kiener Sarah , Troyer Heather , Ruvolo Daniel , Grest Paula , Soto Sara , Letko Anna , Jagannathan Vidhya , Leeb Tosso , Mauldin Elizabeth A. , Yang Ching , Rostaher Ana
Published: September 2023

Epidermolysis bullosa (EB), characterized by defective adhesion of the epidermis to the dermis, is a heterogeneous disease with many subtypes in human patients and domestic animals. We investigated two unrelated cats with recurring erosions and ulcers on ear pinnae, oral mucosa, and paw pads that were suggestive of EB. Histopathology confirmed the diagnosis of EB in both cats. Case 1 was severe and had to be euthanized at 5 months of age. Case 2 had a milder course and was alive at 11 years of age at the time of writing. Whole genome sequencing of both affected cats revealed independent homozygous variants in COL17A1 encoding the collagen type XVII alpha 1 chain. Loss of function variants in COL17A1 lead to junctional epidermolysis bullosa (JEB) in human patients. The identified splice site variant in case 1, c.3019+1del, was predicted to lead to a complete deficiency in collagen type XVII. Case 2 had a splice region variant, c.769+5G>A. Assessment of the functional impact of this variant on the transcript level demonstrated partial aberrant splicing with residual expression of wildtype transcript. Thus, the molecular analyses provided a plausible explanation of the difference in clinical severity between the two cases and allowed the refinement of the diagnosis in the affected cats to JEB. This study highlights the complexity of EB in animals and contributes to a better understanding of the genotype-phenotype correlation in COL17A1-related JEB.
As Seen from the Camera Obscura: Haniya Yutaka’s Ontological Film Theory
Authors: Yamamoto Naoki
Published: September 2023

Haniya Yutaka (1909–1997) was one of the leading figures in postwar Japanese literature and avant-garde art movements, chiefly remembered today for his unfinished metaphysical novel Dead Souls [Shirei, 1946–1997]. This essay, however, examines his hitherto unknown theoretical writings on film. Haniya and other writers gathering around the literary magazine Kindai bungaku [Modern Literature, 1946–1964] shared a keen interest in film’s unparalleled importance in twentieth-century modernity. And their collective efforts to transgress conventional boundaries between literature and film culminated in the 1957 publication of the anthology entitled Literary Film Theory [Bungakuteki eigaron]. Above all, Haniya’s film writing was clearly distinguished for its tendency to explicate film’s paradoxical mode of existence philosophically, an approach that the film critic Matsuda Masao later called an “ontological film theory” [sonzaironteki eigaron]. Looking closely at his essays and interviews collected in Literary Film Theory and two other volumes on this topic—Thoughts in the Darkness [Yami no naka no shisō, 1962] and Dreaming in the Darkness [Yami no naka no musō, 1982]—the present essay reads Haniya’s theorization of cinema in relation to both Martin Heidegger’s existential phenomenology and recent scholarly debates on non-Western film theory.
Deep Neural Network Regression to Assist Non-Invasive Diagnosis of Portal Hypertension
Authors: Baldisseri Federico , Wrona Andrea , Menegatti Danilo , Pietrabissa Antonio , Battilotti Stefano , Califano Claudia , Cristofaro Andrea , Di Giamberardino Paolo , Facchinei Francisco , Palagi Laura , Giuseppi Alessandro , Delli Priscoli Francesco
Published: September 2023

Portal hypertension is a complex medical condition characterized by elevated blood pressure in the portal venous system. The conventional diagnosis of such disease often involves invasive procedures such as liver biopsy, endoscopy, or imaging techniques with contrast agents, which can be uncomfortable for patients and carry inherent risks. This study presents a deep neural network method in support of the non-invasive diagnosis of portal hypertension in patients with chronic liver diseases. The proposed method utilizes readily available clinical data, thus eliminating the need for invasive procedures. A dataset composed of standard laboratory parameters is used to train and validate the deep neural network regressor. The experimental results exhibit reasonable performance in distinguishing patients with portal hypertension from healthy individuals. Such performances may be improved by using larger datasets of high quality. These findings suggest that deep neural networks can serve as useful auxiliary diagnostic tools, aiding healthcare professionals in making timely and accurate decisions for patients suspected of having portal hypertension.
Association between Sleep Timing, Being Overweight and Meal and Snack Consumption in Children and Adolescents in Southern Brazil
Authors: Roberto Denise Miguel Teixeira , Pereira Luciana Jeremias , Vieira Francilene Gracieli Kunradi , Di Pietro Patricia Faria , de Assis Maria Alice Altenburg , Hinnig Patrícia de Fragas
Published: September 2023

Sleep timing is one of the dimensions of sleep that refers to the time of day when sleep occurs. It has been included in sleep-related research because of the potential associations between being overweight and the consumption of meals and snacks. This cross-sectional study aimed to investigate associations between sleep timing, meal and snack consumption and weight status in 1333 schoolchildren aged 7–14 years. The midpoint of sleep was used as a sleep timing measure obtained by the midpoint between bedtime and wake-up time, classified as Early, Intermediate, and Late. Schoolchildren in the Early group were less likely to be overweight (OR: 0.83, 95% CI 0.69; 0.99), and had higher odds of mid-morning snack consumption (OR: 1.95, 95% CI 1.56; 2.44) and lower probability to consume an evening snack (OR: 0.75, 95% CI 0.59; 0.94) compared with the Intermediate group. The Late group had lower odds of mid-morning snack consumption (OR: 0.67, 95% CI 0.55, 0.80) than the Intermediate group. The consumption of mid-morning and evening snacks was associated with the Early and Late midpoints of sleep. These results suggest that bedtime and wake-up time are relevant to consuming meals and snacks and may also be related to a greater probability of being overweight in children and adolescents.
Changes in Food Consumption, BMI, and Body Composition in Youth in the US during the COVID-19 Pandemic
Authors: Moursi Nasreen , Tanofsky-Kraff Marian , Parker Megan , Loch Lucy , Bloomer Bess , Te-Vazquez Jennifer , Nwosu Ejike , Lazareva Julia , Yang Shanna B. , Turner Sara , Brady Sheila , Yanovski Jack
Published: September 2023

Rates of childhood overweight/obesity have risen for decades; however, data show the prevalence increased at a faster rate during the COVID-19 pandemic. Pandemic-associated increases in youth’s body mass index (BMI; kg/m2) have been attributed to decreases in reported physical activity; few studies have examined changes in food intake. We therefore examined changes in total energy, nutrient consumption, BMI, BMIz, and adiposity longitudinally over 3 years, comparing healthy youth aged 8–17 years assessed twice prior to the pandemic, to youth seen once before and once during the pandemic. The total energy intake and percent macronutrient consumption were assessed using a standardized, laboratory-based, buffet-style meal. Height and weight were measured and adiposity was collected via dual energy X-ray absorptiometry. Generalized linear model univariate analyses investigated differences between groups. One-hundred-fifteen youth (15.6 + 2.8 years 47.8% female; 54.8% White) from the Washington D.C., Maryland, and Virginia greater metropolitan area participated. In this secondary analysis, neither changes in total energy intake (p = 0.52) nor changes in nutrient consumption were significantly different between the two groups (ps = 0.23–0.83). Likewise, changes in BMI, BMIz, and adiposity (ps = 0.95–0.25) did not differ by group. Further research should investigate food intake and body composition, comparing youth with and without overweight/obesity to better identify those at greatest risk of excess weight gain during the pandemic.
Measuring Māori Health, Wellbeing, and Disability in Aotearoa Using a Web-Based Survey Methodology
Authors: Ingham Tristram R. , Jones Bernadette , Perry Meredith , von Randow Martin , Milne Barry , King Paula T. , Nikora Linda W. , Sporle Andrew ,   Te Ao Mārama Study Group
Published: September 2023

High-quality evidence on the prevalence and impact of health, wellbeing, and disability among Māori, and other Indigenous peoples, is crucial for mitigating health inequities. Current surveys are predominantly centred within a biomedical paradigm, with the constructs mismatched with Indigenous worldviews. We aimed to develop and deploy an accessible and culturally grounded survey exploring Māori health, wellbeing, and disability using a Kaupapa Māori Research methodology. An extensive codesign process with Māori community partners interrogated all aspects of the design to ensure the process and outcomes met the needs of Māori. A large-scale, nationally representative survey of people of Māori descent was conducted. We used a multi-modal deployment approach that included online and alternate methods of completion. Our analysis included a novel dual-weighting system to ensure generalisability of results to the national Māori population. This achieved a survey of 7230 participants, a sample size comparable with government-administered surveys. The response rate was 11.1%, with 7.3% opting for alternate methods. A high completion rate of 93.4% was observed. This approach demonstrated a high level of engagement, resulting in an unprecedented collection of Māori health, wellbeing, and disability data. This highlights the importance of Indigenous codesign for ensuring accessible and culturally appropriate survey methods.
Trees, Climate Change, and Health: An Urban Planning, Greening and Implementation Perspective
Authors: Woodward Alistair , Hinwood Andrea , Bennett Daniel , Grear Brenton , Vardoulakis Sotiris , Lalchandani Neha , Lyne Katrina , Williams Carmel
Published: September 2023

The In Conversation: Boundary, Spanners, Thinkers and Policy Actors Round Table Series provides a platform for researchers, policy actors, and implementation experts to elevate discussion on emerging issues, present new and upcoming research, and facilitate conversations around impacts and possible solutions. This brief report, on trees, climate change, and health, reflects a conversation between the authors of this paper, along with supporting literature. It explores the potential of green spaces and trees as a viable strategy to address climate change challenges and simultaneously improve population health, well-being, and health equity. In particular, it highlights the public health benefits of trees and green space, the challenges faced in urban areas, and opportunities for the protection, maintenance and regeneration of urban green space.
Silibinin Downregulates Types I and III Collagen Expression via Suppression of the mTOR Signaling Pathway
Authors: Choi Sooyeon , Ham Seoyoon , Lee Young In , Kim Jihee , Lee Won Jai , Lee Ju Hee
Published: September 2023

Keloid scars are fibro-proliferative conditions characterized by abnormal fibroblast proliferation and excessive extracellular matrix deposition. The mammalian target of the rapamycin (mTOR) pathway has emerged as a potential therapeutic target in keloid disease. Silibinin, a natural flavonoid isolated from the seeds and fruits of the milk thistle, is known to inhibit the mTOR signaling pathway in human cervical and hepatoma cancer cells. However, the mechanisms underlying this inhibitory effect are not fully understood. This in vitro study investigated the effects of silibinin on collagen expression in normal human dermal and keloid-derived fibroblasts. We evaluated the effects of silibinin on the expressions of collagen types I and III and assessed its effects on the suppression of the mTOR signaling pathway. Our findings confirmed elevated mTOR phosphorylation levels in keloid scars compared to normal tissue specimens. Silibinin treatment significantly reduced collagen I and III expressions in normal human dermal and keloid-derived fibroblasts. These effects were accompanied by the suppression of the mTOR signaling pathway. Our findings suggest the potential of silibinin as a promising therapeutic agent for preventing and treating keloid scars. Further studies are warranted to explore the clinical application of silibinin in scar management.
Effects of Benzo[a]pyrene on Human Sperm Functions: An In Vitro Study
Authors: Traini Giulia , Tamburrino Lara , Ragosta Maria Emanuela , Guarnieri Giulia , Morelli Annamaria , Vignozzi Linda , Baldi Elisabetta , Marchiani Sara
Published: September 2023

Benzo(a)pyrene (BaP) is considered one of the most dangerous air pollutants for adverse health effects, including reproductive toxicity. It is found both in male and female reproductive fluids likely affecting spermatozoa after the selection process through cervical mucus, a process mimicked in vitro with the swim-up procedure. In vitro effects of BaP (1, 5, 10 µM) were evaluated both in unselected and swim-up selected spermatozoa after 3 and 24 h of incubation. BaP reduced total, progressive and hyperactivated motility and migration in a viscous medium both in swim-up selected and unselected spermatozoa. Viability was not significantly affected in swim-up selected but was reduced in unselected spermatozoa. In swim-up selected spermatozoa, increases in the percentage of spontaneous acrosome reaction and DNA fragmentation were observed after 24 h of incubation, whereas no differences between the control and BaP-treated samples were observed in caspase-3 and -7 activity, indicating no effects on apoptotic pathways. ROS species, evaluated by staining with CellROX® Orange and Dihydroethidium, did not differ in viable spermatozoa after BaP treatment. Conversely, the percentage of unviable ROS-positive spermatozoa increased. Our study suggests that BaP present in male and female genital fluids may heavily affect reproductive functions of human spermatozoa.
Recent Developments in Ion Channel and Ion-Related Signaling
Authors: Ohya Susumu
Published: September 2023

Ion channels play an important role in the cellular functions of various organ systems, such as the nervous, cardiovascular, immune, and endocrine systems, and are potential therapeutic targets for treatments of their dysfunctions, via ‘channelopathy’ [...]
Prolonged Inhibition of the MEK1/2-ERK Signaling Axis Primes Interleukin-1 Beta Expression through Histone 3 Lysine 9 Demethylation in Murine Macrophages
Authors: Low Rachel , Ha Soon-Duck , Sleapnicov Nichita , Maneesh Parthiv , Kim Sung Ouk
Published: September 2023

Macrophages undergo different cellular states upon activation that can be hyporesponsive (tolerated) or hyperresponsive (primed or trained) to subsequent stimuli. Epigenetic modifications are known to play key roles in determining these cellular states. However, little is known about the role of signaling pathways that lead to these epigenetic modifications. Here, we examined the effects of various inhibitors targeting key signaling pathways induced by lipopolysaccharide (LPS) on tolerance and priming in murine macrophages. We found that a prolonged inhibition (>18 h) of the mitogen-activated protein kinase (MEK)1/2—extracellular signal-regulated kinase (ERK)1/2 signaling axis reversed tolerance and primed cells in expressing interleukin (IL)-1β and other inflammatory cytokines such as IL-6, tumor necrosis factor (TNF)α, and CXCL10. The ectopic expression of catalytically active and inactive MEK1 mutants suppressed and enhanced IL-1β expression, respectively. A transcriptomic analysis showed that cells primed by the MEK1/2 inhibitor U0126 expressed higher levels of gene sets associated with immune responses and cytokine/chemokine production, but expressed lower levels of genes with cell cycle progression, chromosome organization, and heterochromatin formation than non-primed cells. Of interest, the mRNA expressions of the histone 3 lysine 9 (H3K9) methyltransferase Suv39h1 and the H3K9 methylation reader Cbx5 were substantially suppressed, whereas the H3K9 demethylase Kdm7a was enhanced, suggesting a role of the MEK1/2-ERK signaling axis in H3K9 demethylation. The H3K9 trimethylation levels in the genomic regions of IL-1β, TNFα, and CXCL10 were decreased by U0126. Also, the H3K9 methyltransferase inhibitor BIX01294 mimicked the U0126 training effects and the overexpression of chromobox homolog (CBX)5 prevented the U0126 training effects in both RAW264.7 cells and bone-marrow-derived macrophages. Collectively, these data suggest that the prolonged inhibition of the MEK1/2-ERK signaling axis reverses tolerance and primed macrophages likely through decreasing the H3K9 methylation levels.
SUCCEED: Sharing Upcycling Cases with Context and Evaluation for Efficient Software Development
Authors: Nakata Takuya , Chen Sinan , Saiki Sachio , Nakamura Masahide
Published: September 2023

Software upcycling, a form of software reuse, is a concept that efficiently generates novel, innovative, and value-added development projects by utilizing knowledge extracted from past projects. However, how to integrate the materials derived from these projects for upcycling remains uncertain. This study defines a systematic model for upcycling cases and develops the Sharing Upcycling Cases with Context and Evaluation for Efficient Software Development (SUCCEED) system to support the implementation of new upcycling initiatives by effectively sharing cases within the organization. To ascertain the efficacy of upcycling within our proposed model and system, we formulated three research questions and conducted two distinct experiments. Through surveys, we identified motivations and characteristics of shared upcycling-relevant development cases. Development tasks were divided into groups, those that employed the SUCCEED system and those that did not, in order to discern the enhancements brought about by upcycling. As a result of this research, we accomplished a comprehensive structuring of both technical and experiential knowledge beneficial for development, a feat previously unrealizable through conventional software reuse, and successfully realized reuse in a proactive and closed environment through construction of the wisdom of crowds for upcycling cases. Consequently, it becomes possible to systematically perform software upcycling by leveraging knowledge from existing projects for streamlining of software development.
Learnability in Automated Driving (LiAD): Concepts for Applying Learnability Engineering (CALE) Based on Long-Term Learning Effects
Authors: Mbelekani Naomi Y. , Bengler Klaus
Published: September 2023

Learnability in Automated Driving (LiAD) is a neglected research topic, especially when considering the unpredictable and intricate ways humans learn to interact and use automated driving systems (ADS) over the sequence of time. Moreover, there is a scarcity of publications dedicated to LiAD (specifically extended learnability methods) to guide the scientific paradigm. As a result, this generates scientific discord and, thus, leaves many facets of long-term learning effects associated with automated driving in dire need of significant research courtesy. This, we believe, is a constraint to knowledge discovery on quality interaction design phenomena. In a sense, it is imperative to abstract knowledge on how long-term effects and learning effects may affect (negatively and positively) users’ learning and mental models. As well as induce changeable behavioural configurations and performances. In view of that, it may be imperative to examine operational concepts that may help researchers envision future scenarios with automation by assessing users’ learning ability, how they learn and what they learn over the sequence of time. As well as constructing a theory of effects (from micro, meso and macro perspectives), which may help profile ergonomic quality design aspects that stand the test of time. As a result, we reviewed the literature on learnability, which we mined for LiAD knowledge discovery from the experience perspective of long-term learning effects. Therefore, the paper offers the reader the resulting discussion points formulated under the Learnability Engineering Life Cycle. For instance, firstly, contextualisation of LiAD with emphasis on extended LiAD. Secondly, conceptualisation and operationalisation of the operational mechanics of LiAD as a concept in ergonomic quality engineering (with an introduction of Concepts for Applying Learnability Engineering (CALE) research based on LiAD knowledge discovery). Thirdly, the systemisation of implementable long-term research strategies towards comprehending behaviour modification associated with extended LiAD. As the vehicle industry revolutionises at a rapid pace towards automation and artificially intelligent (AI) systems, this knowledge is useful for illuminating and instructing quality interaction strategies and Quality Automated Driving (QAD).
An Investigation of Wave-Driven Current Characteristics across Fringing Reefs under Monochromatic Waves
Authors: Yuan Tao , Yao Yu , Li Zhuangzhi , Xu Conghao
Published: September 2023

The aim of this study is to better understand cross-reef wave-driven current characteristics, which are crucial to biological, ecological, and geomorphological processes within coral reefs. This study reports a set of new wave flume measurements to assess flow along the water depth and across a fringing reef profile under the action of a plunging breaker. Laboratory results are presented in view of cross-reef variations in both the wave height and the mean water level (MWL); the vertical profiles of wave-averaged mean currents below the wave trough and along the reef are also presented. To resolve the two-dimensional vertical (2DV) flow characteristics across the reef, Reynolds-Averaged Navier–Stokes (RANS) equations were solved using k-ω SST closure, modified to improve stability, and a Volume of Fluid (VOF) approach was used to capture the water surface. This numerical model was first validated via experimental measurements in view of waves and flows. It was then used to analyze the cross-reef distributions of the mean flow field, turbulent kinetic energy (TKE), and Reynolds shear stress across the reef.
The Fungal, Nutritional, and Metabolomic Diagnostics of the Oil Palm Elaeis guineensis Affected by Bud Rot Disease in Esmeraldas, Ecuador
Authors: Mihai Raluca A. , Melo Heras Erly J. , Landazuri Abarca Pablo A. , Catana Rodica D.
Published: September 2023

The oil palm Elaeis guineensis represents one of the most important crops in Ecuador. Considering that bud rot disease is deadly in Ecuador, more attention has been given to identifying possible causes for palm debility from this disease. We studied the involvement of fungi and nutrients in triggering bud rot disease in E. guineensis. Special emphasis was given to the molecules synthesized by the plant to protect against this devastating disease. Techniques like Diagnosis and Recommendation Integrated System (DRIS) and metagenomic analysis were used to understand the possible implications of biotic and abiotic factors in the development of bud rot disease in oil palm in Ecuador. Liquid chromatography-mass spectrometry (LC-MS) analysis was used to identify the phenolic protection barrier of the palm facing the disease. Our results indicate that fungi from Ascomyceta phylum were found in the tested samples. The species directly involved are different in soil compared with plants. The results indicate a deficiency of chemical elements, such as Ca, Mn, Mg, and Fe, which are responsible for palm debility from bud rot disease. More than 30 compounds with protective roles were identified in the leaves of symptomatic plants from the first stage of the infection.
Could Food Delivery Involve Certified Quality Products? An Innovative Case Study during the SARS-CoV-2 Pandemic in Italy
Authors: Rapa Mattia , Giannetti Vanessa , Boccacci Mariani Maurizio , Di Francesco Federico , Porpiglia Alessandro
Published: September 2023

This study evaluates the feasibility of a new food delivery service involving only food products with quality certification. In particular, through an ad hoc survey, it evaluates the influence of consumers’ personal characteristics and choice motives on joining this innovative service and the willingness to pay of the respondents. A survey was completely anonymously and voluntarily administered during the SARS-CoV-2 pandemic. A total of 630 answers were collected. Logit and ordinal logit regression were carried out to analyze data. Women and respondents who have more leisure time are more likely to join the service. The analysis of choice motives suggests that consumers more concerned with food quality, and those devoting a higher weekly budget to buying groceries are more likely to be interested in the proposed service. Individuals willing to buy groceries based on certifications and organoleptic properties and people who habitually consume one to five meals outside were more likely to be willing to increase their weekly budget to join the service. To the best of our knowledge, this is the first study evaluating the influence of personal characteristics and choice motives on an innovative food delivery service involving only certified quality products in Italy.
Delineation of Urban Development Boundary and Carbon Emission Effects in Xuzhou City, China
Authors: Ji Haitao , Li Xiaoshun , Geng Yiwei , Chen Xin , Wang Yuexiang , Cheng Jumei , Chen Zhuang
Published: September 2023

Urban development boundary (UDB) has always served as a crucial aspect of urban sprawl research. The objective of this paper is to investigate boundary delineation and carbon emission effects. Firstly, we examined the patterns of land use changes. Additionally, this paper utilized the FLUS model and land use carbon emission calculation model to delineate UDB and calculate carbon emission effects within UDB under typical scenarios. The research results are as follows: (1) Xuzhou city witnessed a significant increase in both forest land and construction land from 2010 to 2020. (2) Under the CPS, the area of cultivated land increased by 217.05 km2 compared to the NDS. The UDB area under the NDS and CPS was 971.50 km2 and 968.99 km2, respectively. (3) Compared to the NDS, the CPS led to a net carbon emission increase of 4759.93 t within the UDB. Therefore, we should enhance the carbon sequestration and emission reduction capacity of the agricultural system. This study is beneficial for expanding the depth of research on the UDB and guiding the low-carbon urban development. We sincerely encourage readers to download this paper to improve this paper.