bluuebunny/crossref_metadata_2025 huggingface dataset:
Oregano Oil, Epsilon-Polylysine and Citric Acid Assisted Inactivation of Salmonella in Two Kinds of Tahini during Thermal Treatment and Storage
Authors: Xu Yuanmei , Guan Xiangyu , Lin Biying , Li Rui , Wang Shaojin
Published: June 2021

Tahini and tahini-based products are popular with consumers due to their special flavor and high nutritional values, but often have been linked to Salmonella outbreaks. The objective of this study was to compare effects of different kinds of natural antimicrobials on Salmonella inactivation in undiluted and diluted tahini during thermal treatment and storage. Results showed that the Weibull model was more suitable to describe the thermal inactivation behavior of S. montevideo CICC21588 in two kinds of tahini than the first-order model. Inactivation curves were concave-upward in undiluted tahini but concave-downward in diluted tahini. During storage of undiluted tahini, 3% oregano oil caused extra 1.44 or 0.80 log CFU/g reductions after 7 days at 25 °C or 4 °C compared to the control and 0.5% citric acid caused an extra reduction of 0.75 log CFU/g after 7 d at 4 °C. For diluted tahini, 2–3% oregano oil and 0.4–0.5% ε-polylysine reduced more populations compared to undiluted tahini. These antimicrobials all inhibited the growth of S. montevideo during 24 h at 25 °C and ε-polylysine had the best effect. Furthermore, these antimicrobials enhanced the Salmonella inactivation in diluted tahini during thermal treatment, and there was less of a synergistic effect of thermal and antimicrobials in undiluted tahini due to less sublethal injured cells caused by heat. This study may provide useful information for Salmonella inactivation in tahini.
A Consumer Segmentation Study for Meat and Meat Alternatives in Switzerland
Authors: Götze Franziska , Brunner Thomas A.
Published: June 2021

The aim of this study was to identify consumer groups regarding meat and meat alternatives, which are homogeneous in themselves but very different from one another. To date, the literature has analysed the attitudes towards, and the motives behind, the consumption of meat and meat alternatives. However, segmentation research portraying homogeneous consumer groups that are consuming or willing to consume meat alternatives is lacking. This study closes this research gap and, in doing so, also shows how meat consumption is related to the consumption of alternative products. A questionnaire was sent out to a random sample in the German- and French-speaking parts of Switzerland, resulting in 561 responses. A hierarchical cluster analysis using seven scales revealed six distinct consumer groups, which covered all types of consumers, from the uncompromising meat-eater to the health-conscious meat avoider. The results show that meat alternatives are not always consumed as a substitute for meat but can also be a complementary component in one’s diet. This study contributes to the scientific literature by providing useful information for the food industry involved in producing and marketing meat and meat alternatives to different target groups.
Comparison of Home Use Tests with Differing Time and Order Controls
Authors: Lee Nahyung , Lee Jeehyun
Published: June 2021

Consumer tests are classified in terms of the location of testing as laboratory tests or central location tests (CLTs) and home use tests (HUTs). CLT is generally used in sensory tests due to the ease of test control, whereas HUT has higher validity because of real consumption. However, the lack of test control in HUT is a major issue. In order to investigate the error occurrence and efforts required to minimize errors, three groups of tests were designed differing time and order control and evaluation was conducted using six snacks with texture differences. Errors related to time, order, and consumer or sample number were higher for more controlled conditions, however, most errors were recoverable using identification information except for cases of no response. Additionally, consumers preferred to consume all snacks in the evening at home, which differed from the typical 9 a.m. to 6 p.m. evaluation time in CLT. However, the timing differed for consumers with self-reported snacking time. The research title that included the term ‘home’ might have influenced the participants’ choice of location for evaluation. Overall, there was no significant difference between the results of groups despite different time and order controls, which could increase the applicability of HUT.
Supplementation of Double Cream Cheese with Allium roseum: Effects on Quality Improvement and Shelf-Life Extension
Authors: Gliguem Hela , Ben Hassine Dorsaf , Ben Haj Said Leila , Ben Tekaya Imene , Rahmani Rami , Bellagha Sihem
Published: June 2021

This study gives a new insight into the direct supplementation of Allium roseum leaves in double cream cheese. Allium roseum leaves were added to double cream cheese as a powder and a fresh paste. Based on the formulation calculation and on sensory analyses, doses of 6% paste and 0.8% powder were used to formulate the flavored cheeses. The sensory characteristics of the two double cream cheeses were assessed and compared to a plain formula. Cheese samples, stored at 5 °C, were sampled every 0, 4, 8, 12 and 15 days and analyzed for pH, fat, dry contents, yeasts and molds and total coliforms. The positive effects of Allium roseum supplementation, either as a powder or as a fresh paste, have been proven, based on sensorial, physicochemical, and microbiological qualities. The shelf life of cheese samples was also determined through an accelerated shelf life test and the Arrhenius equation. The experiments were conducted at 5, 15, and 25 °C for 15 days. The results showed a significant shelf life extension for flavored double cream cheeses (12 days) versus the plain formula (10 days). The use of Allium roseum leaves, as a natural preservative, seems to be a promising trend for the formulation of similar dairy products.
Fabrication and Characterization of Gelatin/Zein Nanofiber Films Loading Perillaldehyde for the Preservation of Chilled Chicken
Authors: Wang Debao , Liu Yini , Sun Jinyue , Sun Zhilan , Liu Fang , Du Lihui , Wang Daoying
Published: June 2021

Perillaldehyde is a natural antibacterial agent extracted from perilla essential oil. In our methodology, five antibacterial nanofiber packaging films are prepared by loading different concentrations of perillaldehyde (P) into gelatin/zein (G/Z) polymers. Morphology observations show that the G/Z/P film had a good uniform microstructure and nano-diameter as the weight ratio of 5:1:0.02 (G/Z/P). Fourier transform infrared spectroscopy, and X-ray indicate that these three ingredients had good compatibility and strong interaction via hydrogen bonding. Water contact angle results show that the G/Z/P films gradually change from hydrophilic to hydrophobic with the increase of perillaldehyde. Thermal analysis indicates that the G/Z/P (5:1:0.02) film has good thermal stability. Antibacterial and storage analysis indicates that G/Z/P (5:1:0.02) film is effective to inactivate Staphylococcus aureus and Salmonella enteritidis, and obviously reduces the increasing rate of total bacteria counts and volatile basic nitrogen of chicken breasts. This study indicates that the G/Z/P (5:1:0.02) is a kind of potential antibacterial food packaging film.
Transformation of Inferior Tomato into Preservative: Fermentation by Multi-Bacteriocin Producing Lactobacillus paracasei WX322
Authors: Zhu Rong , Liu Xiaoqing , Li Xiaofen , Zeng Kaifang , Yi Lanhua
Published: June 2021

Loss and waste of postharvest vegetables are the main challenges facing the world’s vegetable supply. In this study, an innovative method of value-added transformation was provided: production of bacteriocin from vegetable waste, and then its application to preservation of vegetables. Antibacterial activity to soft rot pathogen Pectobacterium cartovorum (Pcb BZA12) indicated that tomato performed best in the nutrition supply for bacteriocin production among 12 tested vegetables. Moreover, the antibacterial activity was from Lactobacillus paracasei WX322, not components of vegetables. During a fermentation period of 10 days in tomato juice, L. paracasei WX322 grew well and antibacterial activity reached the maximum on the tenth day. Thermostability and proteinase sensitivity of the bacteriocin from tomato juice were the same with that from Man-Rogosa-Sharpe broth. Scanning electron microscope images indicated that the bacteriocin from tomato juice caused great damage to Pcb BZA12. At the same time, the bacteriocin from tomato juice significantly reduced the rotten rate of Chinese cabbage from 100% ± 0% to 20% ± 8.16% on the third day during storage. The rotten rate decrease of cucumber, tomato, and green bean was 100% ± 0% to 0% ± 0%, 70% ± 14.14% to 13.33% ± 9.43%, and 76.67% ± 4.71% to 26.67% ± 4.71%, respectively. Bacteriocin treatment did not reduce the rotten rate of balsam pear, but alleviated its symptoms.
Isolation and Identification of Natural Colorant Producing Soil-Borne Aspergillus niger from Bangladesh and Extraction of the Pigment
Authors: Toma Maria Afroz , Nazir K H M Nazmul Hussain , Mahmud Md. Muket , Mishra Pravin , Ali Md. Kowser , Kabir Ajran , Shahid Md. Ahosanul Haque , Siddique Mahbubul Pratik , Alim Md. Abdul
Published: June 2021

Natural colorants have been used in several ways throughout human history, such as in food, dyes, pharmaceuticals, cosmetics, and many other products. The study aimed to isolate the natural colorant-producing filamentous fungi Aspergillus niger from soil and extract pigments for its potential use specially for food production. Fourteen soil samples were collected from Madhupur National Park at Madhupur Upazila in the Mymensingh district, Bangladesh. The Aspergillus niger was isolated and identified from the soil samples by following conventional mycological methods (cultural and morphological characteristics), followed by confirmatory identification by a polymerase chain reaction (PCR) of conserved sequences of ITS1 ribosomal DNA using specific oligonucleotide primers. This was followed by genus- and species-specific primers targeting Aspergillus niger with an amplicon size of 521 and 310 bp, respectively. For pigment production, a mass culture of Aspergillus niger was conducted in Sabouraud dextrose broth in shaking conditions for seven days. The biomass was subjected to extraction of the pigments following an ethanol-based extraction method and concentrated using a rotary evaporator. Aspergillus niger could be isolated from three samples. The yield of extracted brown pigment from Aspergillus niger was 0.75% (w/v). Spectroscopic analysis of the pigments was carried out using a UV–VIS spectrophotometer. An in vivo experiment was conducted with mice to assess the toxicity of the pigments. From the colorimetric and sensory evaluations, pigment-supplemented products (cookies and lemon juice) were found to be more acceptable than the control products. This could be the first attempt to use Aspergillus niger extracted pigment from soil samples in food products in Bangladesh, but for successful food production, the food colorants must be approved by a responsible authority, e.g., the FDA or the BSTI. Moreover, fungal pigments could be used in the emerging fields of the food and textile industries in Bangladesh.
Prediction of Esophageal Varices Based on Serum-Ascites Albumin Gradient in Cirrhotic Patients
Authors: Thong Vo Duy , Anh Ho Thi Van
Published: June 2021

Background: Ascites and esophageal varices (EV) are the most common complications of portal hypertension, with an incidence of approximately 50%. Since effective preventive modalities have been established for variceal hemorrhage, early detection of EV is critical for primary prevention of bleeding. Serum-ascites albumin gradient (SAAG) can be considered an indirect parameter for the detection of EV and is useful in regions where there is a shortage of human and material resources to perform upper gastrointestinal endoscopy (UGE). The aim was to evaluate the role of serum-ascites albumin gradient (SAAG) in the prediction of esophageal varices (EV) in cirrhotic patients with ascites. Methods: All cirrhotic patients with ascites, identified by ultrasonography, who underwent measurement of SAAG, were included in this study. All patients underwent upper gastrointestinal endoscopy (UGE) for assessment of the presence and size of EV. Results: The study included 80 cirrhotic patients with ascites. The main causes of cirrhosis were alcohol intake (37.5%), hepatitis B virus (25.0%), and hepatitis C virus (15.0%). Patients with SAAG values > 1.75 g/dL demonstrated EV with a sensitivity and specificity of 78.4% and 83.3%, respectively. SAAG values > 1.8 g/dL were associated with the risk of large EV with AUC of 0.856, sensitivity of 88.24%, and specificity of 50.79%. The correlation coefficient (r) between SAAG and EV was 0.429, which was statistically significant (p < 0.001). Conclusions: Cirrhotic patients with SAAG values ≥ 1.8 have a higher risk of large EV. In particular, those with values > 1.9 who have higher possibility of bleeding must undergo upper GI endoscopy.
Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction
Authors: Natonek-Wiśniewska Małgorzata , Krzyścin Piotr , Rubiś Dominika
Published: June 2021

Species identification of the components of various duck breeds has revealed that the lowest identifiable number of components depends on the breed. The results (shown on the agarose gel) of a species-specific PCR reaction for Rouen ducks were less intense than the results for the same amount of components from other popular duck breeds, suggesting differences in the Rouen duck genome. Therefore, the present study aimed to identify part of the Rouen duck’s gene sequences and to develop two new primer pairs. The first pair enables breed-independent identification of duck DNA, and the second distinguishes Rouen ducks from Chinese and Indian Runner ducks. The sequencing reaction yielded sequences of 1386 bp in length, and the identified sequence differs by around 7% from the sequences of Chinese duck species. The detected sequence contributes to improving species identification methods for duck DNA. On its basis, two primers for the identification of duck DNA were designed. The first allows for DNA amplification with the same sensitivity regardless of duck breed. The second primer’s pair is breed specific, and it distinguishes Rouen ducks from Chinese and Indian Runner ducks. Both methods are very sensitive (0.05%).
Genotyping-by-Sequencing-Based Genome-Wide Association Studies of Fusarium Wilt Resistance in Radishes (Raphanus sativus L.)
Authors: Lee O New , Koo Hyunjin , Yu Jae Woong , Park Han Yong
Published: June 2021

Fusarium wilt (FW) is a fungal disease that causes severe yield losses in radish production. The most effective method to control the FW is the development and use of resistant varieties in cultivation. The identification of marker loci linked to FW resistance are expected to facilitate the breeding of disease-resistant radishes. In the present study, we applied an integrated framework of genome-wide association studies (GWAS) using genotyping-by-sequencing (GBS) to identify FW resistance loci among a panel of 225 radish accessions, including 58 elite breeding lines. Phenotyping was conducted by manual inoculation of seedlings with the FW pathogen, and scoring for the disease index was conducted three weeks after inoculation during two constitutive years. The GWAS analysis identified 44 single nucleotide polymorphisms (SNPs) and twenty putative candidate genes that were significantly associated with FW resistance. In addition, a total of four QTLs were identified from F2 population derived from a FW resistant line and a susceptible line, one of which was co-located with the SNPs on chromosome 7, detected in GWAS study. These markers will be valuable for molecular breeding programs and marker-assisted selection to develop FW resistant varieties of R. sativus.
At the Intersection of the Social and Physical Environments: Building a Model of the Influence of Caregivers and Peers on Direct Engagement with Nature
Authors: Little Sarah , Rice Art
Published: June 2021

The movement to reconnect children to nature touts the many benefits associated with exposure to nature and encourages designers and planners of the physical environment to incorporate more nature into the daily lives of children. However, connecting children with nature may not be as simple as designing more nature into the physical environment. Variables beyond convenient availability of natural environments affect children’s engagement with nature. Of particular interest is the influence of the social environment. The research seeks to build a model to understand the influence of caregivers and peers on a child’s direct engagement with nature. An initial model of social influences was constructed from existing literature and refined from findings from an original research study, a qualitative investigation exploring the highly imaginative and social experience of a group of boys who played in a neighborhood creek (n = 3, boys, n = 2, parents). The most meaningful social influence on the boys’ direct engagement with nature was the level of autonomy granted by caregivers; however, the physical environment supported the autonomy as well. The autonomy afforded an opportunity to navigate risks, forge long-term friendships, and support higher-order cognitive play behavior.
Do Clinical Trials Meet Current Care Needs? Views of Digestive Oncology Specialists in Galicia (Spain) Using the Delphi Method
Authors: Fernández Montes Ana , Martinez-Lago Nieves , de la Cámara Gomez Juan , Brozos Vázquez Elena María , Candamio Folgar Sonia , Carmona Campos Marta , Cousillas Castiñeiras Antía , Covela Rúa Marta , Gallardo Martín Elena , González Villarroel Paula , Graña Suarez Begoña , Jorge Fernández Mónica , Pellón Augusto María Luz , Quintero Aldana Guillermo , Romero Reinoso Carlos , Salgado Fernández Mercedes , Vázquez Rivera Francisca , Ayuso Álvarez Ana , Culqui Dante R. , Méndez Méndez José Carlos ,   RIGhT-sens Working Group
Published: June 2021

Background: In recent years, abundant scientific evidence has been generated based on clinical trials (CT) in the field of oncology. The general objective of this paper is to find out the extent to which decision making is based on knowledge of the most recent CT. Its specific objectives are to pinpoint difficulties with decision making based on the CT performed and find out the motivations patients and clinicians have when taking part in a CT. Methodology: Combined, prospective study, based on the Delphi method. A lack of correspondence between the people who take part in CT and patients who come for consultation has been identified. A need for training in analysing and interpreting CT has also been identified and a lack of trust in the results of CT financed by the pharmaceutical industry itself has been perceived. Conclusions: There is a difficulty in selecting oncological treatment due to the lack of correspondence between the patients included in the CT and patients seen in consultation. In this process, real world data studies may be highly useful, as they may provide this group with greater training in interpreting CT and their results.
Effects of Two Foot-Ankle Interventions on Foot Structure, Function, and Balance Ability in Obese People with Pes Planus
Authors: Park Du-Jin , Lee Kyung-Sun , Park Se-Yeon
Published: June 2021

Obese people are prone to foot deformities such as flat feet. Foot management programs are important to prevent them. This study investigated the effects of two foot-ankle interventions on balance ability, foot arch, ankle strength, plantar fascia thickness, and foot functions in obese people with pes planus for four weeks. The experiment was designed as a randomized controlled trial. Twenty-four participants who met the inclusion criteria were selected, and they were randomly assigned to either a short foot group (SFG) or proprioceptive neuromuscular facilitation group (PNFG) according to foot-ankle intervention. Two interventions were commenced three times a week for 20 min over four weeks. The tests were conducted at two intervals: pre-intervention and at four weeks. The tests were conducted in the following order: the patient-specific functional scale test (PSFS), an ultrasound of the plantar fascia, the navicular drop test, balance test, and the four-way ankle strength test. Two groups showed significant differences in balance ability, foot arch, ankle strength, plantar fascia thickness, and foot functions between pre-test and post-test (p < 0.05). PNFG had significantly higher dorsiflexor and invertor strength than SFG (p < 0.05). SF and PNF interventions were effective to improve balance ability, foot arch, ankle strength, plantar fascia thickness, and foot functions in obese people with pes planus. Additionally, PNF intervention is more beneficial in increasing the dorsiflexor and invertor strength compared to SF intervention.
A Review on Plant Responses to Salt Stress and Their Mechanisms of Salt Resistance
Authors: Hao Shanhu , Wang Yiran , Yan Yunxiu , Liu Yuhang , Wang Jingyao , Chen Su
Published: June 2021

Nowadays, crop insufficiency resulting from soil salinization is threatening the world. On the basis that soil salinization has become a worldwide problem, studying the mechanisms of plant salt tolerance is of great theoretical and practical significance to improve crop yield, to cultivate new salt-tolerant varieties, and to make full use of saline land. Based on previous studies, this paper reviews the damage of salt stress to plants, including suppression of photosynthesis, disturbance of ion homeostasis, and membrane peroxidation. We have also summarized the physiological mechanisms of salt tolerance, including reactive oxygen species (ROS) scavenging and osmotic adjustment. Four main stress-related signaling pathways, salt overly sensitive (SOS) pathway, calcium-dependent protein kinase (CDPK) pathway, mitogen-activated protein kinase (MAPKs) pathway, and abscisic acid (ABA) pathway, are included. We have also enumerated some salt stress-responsive genes that correspond to physiological mechanisms. In the end, we have outlined the present approaches and techniques to improve salt tolerance of plants. All in all, we reviewed those aspects above, in the hope of providing valuable background knowledge for the future cultivation of agricultural and forestry plants.
Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
Authors: Nath Peuli , Kabir Md Alamgir , Doust Somaiyeh Khoubafarin , Ray Aniruddha
Published: June 2021

Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.
Perceived Physical Competence Predicts Gains in Children’s Locomotor but Not Ball Skills across an Intervention
Authors: Palmer Kara K. , Nunu Michael A. , Scott-Andrews Katherine Q. , Robinson Leah E.
Published: June 2021

The purpose of this pre/post experimental study was to examine if children’s perceived physical competence predicted changes in motor skills across an intervention. Sixty-seven children (Mage = 53.2 ± 3.7 months) participated in a 16-week, mastery-climate motor skill intervention. Perceived physical competence was assessed before the intervention using the physical competence subscale of the Pictorial Scale of Perceived Competence and Social Acceptance for Young Children. Motor skills were assessed using the Test of Gross Motor Development-3rd Edition before and after the intervention. Results revealed that controlling for pretest skills, perceived physical competence significantly predicted posttest locomotor (p < 0.05) and total skills (p < 0.05) but did not predict posttest ball skills (p > 0.05). These results indicate that perceived physical competence may be a significant factor that predicts children’s gains in locomotor or total skills, but not ball skills, across an intervention.
Cross-Sectional and Longitudinal Associations between 24-Hour Movement Behaviours, Recreational Screen Use and Psychosocial Health Outcomes in Children: A Compositional Data Analysis Approach
Authors: Chong Kar Hau , Parrish Anne-Maree , Cliff Dylan P. , Dumuid Dorothea , Okely Anthony D.
Published: June 2021

It remains unclear whether the time-use composition of 24-h movement behaviours (sleep, sedentary time (ST), physical activity (PA)) and recreational screen use are independently associated with psychosocial health. This study examined the cross-sectional and longitudinal associations between 24-h movement behaviour composition, recreational screen use and psychosocial health outcomes in children. Measures completed at baseline (n = 127; 11.7 years) and follow-up (n = 88; 12.8 years) included accelerometer-based 24-h movement behaviours, self-reported recreational screen use and psychosocial health (Strengths and Difficulties Questionnaire, Kessler’s Psychological Distress Scale). Linear mixed models were used to examine the cross-sectional and longitudinal associations between the 24-h movement behaviour composition and recreational screen use levels with psychosocial health outcomes. Overall, the movement behaviour composition (p < 0.05) and recreational screen use levels (p < 0.01) were both cross-sectionally but not longitudinally associated with psychosocial health outcomes. Relative to other behaviours, sleep was negatively associated, while light-intensity PA was positively associated with internalising problems and total difficulties scores. ST was positively associated with internalising problems. High levels of recreational screen use (>2 h/day) were associated with greater externalising problems, total difficulties scores and psychological distress. These findings reinforce the importance of achieving a balance between different types of movement behaviours over a 24-h period for psychosocial health.
A Cumulative Framework for Identifying Overburdened Populations under the Toxic Substances Control Act: Formaldehyde Case Study
Authors: Pullen Fedinick Kristi , Yiliqi Ilch , Lam Yukyan , Lennett David , Singla Veena , Rotkin-Ellman Miriam , Sass Jennifer
Published: June 2021

Extensive scholarship has demonstrated that communities of color, low-income communities, and Indigenous communities face greater environmental and health hazards compared to communities with more White or affluent people. Low-income, Indigenous, Black, and/or other populations of color are also more likely to lack access to health care facilities, healthy food, and adequate formal education opportunities. Despite the mountains of evidence that demonstrate the existence and significance of the elevated toxic social and environmental exposures experienced by these communities, the inclusion of these factors into chemical evaluations has been scarce. In this paper, we demonstrate a process built with publicly available data and simple geospatial techniques that could be utilized by the U.S. Environmental Protection Agency (USEPA) to incorporate cumulative approaches into risk assessments under the Toxic Substances Control Act. The use of these approaches, particularly as they relate to identifying potentially exposed and susceptible subpopulations, would help USEPA develop appropriate risk estimates and mitigation strategies to protect disproportionately burdened populations from the adverse effects of chemical exposures. By utilizing such approaches to inform risk evaluation and mitigation, USEPA can identify and protect those most burdened and impacted by toxic chemicals, and finally begin to close the gap of environmental health inequities.
Effectiveness of a Central Discharge Element Sock for Plantar Temperature Reduction and Improving Comfort
Authors: Martínez-Nova Alfonso , Jiménez-Cano Víctor Manuel , Caracuel-López Juan Miguel , Gómez-Martín Beatriz , Escamilla-Martínez Elena , Sánchez-Rodríguez Raquel
Published: June 2021

U-shaped plantar cushions could help reduce stress affecting the central forefoot without the need for an orthosis, but they are yet to be integrated as an element in socks. The objective of this study was to verify the effectiveness of a sock with a central discharge element in terms of plantar temperature and comfort. The sample comprised 38 subjects (13 men and 25 women). Their plantar temperatures were measured with a thermographic camera in a basal situation and after each of two 10-minute walks around an indoor circuit during which they wore either control or experimental socks at random (the same design, weight, and fiber, but with the plantar cushioning element added). After the walks, each subject responded to a comfort questionnaire (five-point Likert scale), blindly scoring the two socks. The highest temperatures (28.3 ± 2.7 °C) were recorded in the zone of the second and third metatarsal heads. With the experimental socks, the observed temperature increase in the central forefoot zone was significantly less than with the control socks (31.6 vs. 30.6 °C, p = 0.001). The subjects found the experimental socks to be more comfortable than the controls (4.63 ± 0.5 vs. 4.03 ± 0.5, p < 0.001). The discharge element included in the experimental socks was effective since it reduced the contact zones and excess friction with the ground, thereby lessening overheating by more than 1 °C. Furthermore, the experimental socks were perceived as being more comfortable by the subjects who had mild and occasional foot discomfort.
The Mental Health and Wellbeing of University Students: Acceptability, Effectiveness, and Mechanisms of a Mindfulness-Based Course
Authors: Medlicott Emma , Phillips Alice , Crane Catherine , Hinze Verena , Taylor Laura , Tickell Alice , Montero-Marin Jesus , Kuyken Willem
Published: June 2021

Mental health problems are relatively common during university and adversely affect academic outcomes. Evidence suggests that mindfulness can support the mental health and wellbeing of university students. We explored the acceptability and effectiveness of an 8-week instructor-led mindfulness-based course (“Mindfulness: Finding Peace in a Frantic World”; Williams and Penman, 2011) on improving wellbeing and mental health (self-reported distress), orientation and motivation towards academic goals, and the mechanisms driving these changes. Eighty-six undergraduate and post-graduate students (>18 years) participated. Students engaged well with the course, with 36 (48.0%) completing the whole programme, 52 (69.3%) attending 7 out of 8 sessions, and 71 (94.7%) completing at least half. Significant improvements in wellbeing and mental health were found post-intervention and at 6-week follow-up. Improvements in wellbeing were mediated by mindfulness, self-compassion, and resilience. Improvements in mental health were mediated by improvements in mindfulness and resilience but not self-compassion. Significant improvements in students’ orientation to their academic goal, measured by “commitment” to, “likelihood” of achieving, and feeling more equipped with the “skills and resources” needed, were found at post-intervention and at 6-week follow-up. Whilst exploratory, the results suggest that this mindfulness intervention is acceptable and effective for university students and can support academic study.
Beyond Behaviour: How Health Inequality Theory Can Enhance Our Understanding of the ‘Alcohol-Harm Paradox’
Authors: Boyd Jennifer , Bambra Clare , Purshouse Robin C. , Holmes John
Published: June 2021

There are large socioeconomic inequalities in alcohol-related harm. The alcohol harm paradox (AHP) is the consistent finding that lower socioeconomic groups consume the same or less as higher socioeconomic groups yet experience greater rates of harm. To date, alcohol researchers have predominantly taken an individualised behavioural approach to understand the AHP. This paper calls for a new approach which draws on theories of health inequality, specifically the social determinants of health, fundamental cause theory, political economy of health and eco-social models. These theories consist of several interwoven causal mechanisms, including genetic inheritance, the role of social networks, the unequal availability of wealth and other resources, the psychosocial experience of lower socioeconomic position, and the accumulation of these experiences over time. To date, research exploring the causes of the AHP has often lacked clear theoretical underpinning. Drawing on these theoretical approaches in alcohol research would not only address this gap but would also result in a structured effort to identify the causes of the AHP. Given the present lack of clear evidence in favour of any specific theory, it is difficult to conclude whether one theory should take primacy in future research efforts. However, drawing on any of these theories would shift how we think about the causes of the paradox, from health behaviour in isolation to the wider context of complex interacting mechanisms between individuals and their environment. Meanwhile, computer simulations have the potential to test the competing theoretical perspectives, both in the abstract and empirically via synthesis of the disparate existing evidence base. Overall, making greater use of existing theoretical frameworks in alcohol epidemiology would offer novel insights into the AHP and generate knowledge of how to intervene to mitigate inequalities in alcohol-related harm.
A Multi-Scale Virtual Terrain for Hierarchically Structured Non-Location Data
Authors: Xin Rui , Ai Tinghua , Zhu Ruoxin , Ai Bo , Yang Min , Meng Liqiu
Published: June 2021

Metaphor are commonly used rhetorical devices in linguistics. Among the various types, spatial metaphors are relatively common because of their intuitive and sensible nature. There are also many studies that use spatial metaphors to express non-location data in the field of visualization. For instance, some virtual terrains can be built based on computer technologies and visualization methods. In virtual terrains, the original abstract data can obtain specific positions, shapes, colors, etc. and people’s visual and image thinking can play a role. In addition, the theories and methods used in the space field could be applied to help people observe and analyze abstract data. However, current research has limited the use of these space theories and methods. For instance, many existing map theories and methods are not well combined. In addition, it is difficult to fully display data in virtual terrains, such as showing the structure and relationship at the same time. Facing the above problems, this study takes hierarchical data as the research object and expresses both the data structure and relationship from a spatial perspective. First, the conversion from high-dimensional non-location data to two-dimensional discrete points is achieved by a dimensionality reduction algorithm to reflect the data relationship. Based on this, kernel density estimation interpolation and fractal noise algorithms are used to construct terrain features in the virtual terrains. Under the control of the kernel density search radius and noise proportion, a multi-scale terrain model is built with the help of level of detail (LOD) technology to express the hierarchical structure and support the multi-scale analysis of data. Finally, experiments with actual data are carried out to verify the proposed method.
The Use of UAV in Cadastral Mapping of the Czech Republic
Authors: Šafář Václav , Potůčková Markéta , Karas Jakub , Tlustý Jan , Štefanová Eva , Jančovič Marián , Cígler Žofková Drahomíra
Published: June 2021

The main challenge in the renewal and updating of the Cadastre of Real Estate of the Czech Republic is to achieve maximum efficiency but to retain the required accuracy of all points in the register. The paper discusses the possibility of using UAV photogrammetry and laser scanning for cadastral mapping in the Czech Republic. Point clouds from images and laser scans together with orthoimages were derived over twelve test areas. Control and check points were measured using geodetic methods (RTK-GNSS and total stations). The accuracy of the detailed survey based on UAV technologies was checked on hundreds of points, mainly building corners and fence foundations. The results show that the required accuracy of 0.14 m was achieved on more than 80% and 98% of points in the case of the image point clouds and orthoimages and the case of the LiDAR point cloud, respectively. Nevertheless, the methods lack completeness of the performed survey that must be supplied by geodetic measurements. The paper also provides a comparison of the costs connected to traditional and UAV-based cadastral mapping, and it addresses the necessary changes in the organisational and technological processes in order to utilise the UAV based technologies.
Methodological Proposal for Automated Detection of the Wildland–Urban Interface: Application to the Metropolitan Regions of Madrid and Barcelona
Authors: Zambrano-Ballesteros Andrea , Nanu Sabina Florina , Navarro-Carrión José Tomás , Ramón-Morte Alfredo
Published: June 2021

Official information on Land Use Land Cover is essential for mapping wildland–urban interface (WUI) zones. However, these resources do not always provide the geometrical or thematic accuracy required to delimit buildings that are easily exposed to risk of wildfire at the appropriate scale. This research shows that the integration of active remote sensing and official Land Use Land Cover (LULC) databases, such as the Spanish Land Use Land Cover information system (SIOSE), creates the synergy capable of achieving this. An automated method was developed to detect WUI zones by the massive geoprocessing of data from official and open repositories of the Spanish national plan for territory observation (PNOT) of the Spanish national geographic institute (IGN), and it was tested in the most important metropolitan zones in Spain: Barcelona and Madrid. The processing of trillions of LiDAR data and their integration with thousands of SIOSE polygons were managed in a Linux environment, with libraries for geographic processing and a PostgreSQL database server. All this allowed the buildings that are exposed to wildfire risk with a high level of accuracy to be obtained with a methodology that can be applied anywhere in the Spanish territory.
V600EBRAF Inhibition Induces Cytoprotective Autophagy through AMPK in Thyroid Cancer Cells
Authors: Jiménez-Mora Eva , Gallego Beatriz , Díaz-Gago Sergio , Lasa Marina , Baquero Pablo , Chiloeches Antonio
Published: June 2021

The dysregulation of autophagy is important in the development of many cancers, including thyroid cancer, where V600EBRAF is a main oncogene. Here, we analyse the effect of V600EBRAF inhibition on autophagy, the mechanisms involved in this regulation and the role of autophagy in cell survival of thyroid cancer cells. We reveal that the inhibition of V600EBRAF activity with its specific inhibitor PLX4720 or the depletion of its expression by siRNA induces autophagy in thyroid tumour cells. We show that V600EBRAF downregulation increases LKB1-AMPK signalling and decreases mTOR activity through a MEK/ERK-dependent mechanism. Moreover, we demonstrate that PLX4720 activates ULK1 and increases autophagy through the activation of the AMPK-ULK1 pathway, but not by the inhibition of mTOR. In addition, we find that autophagy blockade decreases cell viability and sensitize thyroid cancer cells to V600EBRAF inhibition by PLX4720 treatment. Finally, we generate a thyroid xenograft model to demonstrate that autophagy inhibition synergistically enhances the anti-proliferative and pro-apoptotic effects of V600EBRAF inhibition in vivo. Collectively, we uncover a new role of AMPK in mediating the induction of cytoprotective autophagy by V600EBRAF inhibition. In addition, these data establish a rationale for designing an integrated therapy targeting V600EBRAF and the LKB1-AMPK-ULK1-autophagy axis for the treatment of V600EBRAF-positive thyroid tumours.
Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio
Authors: Waghmare Swapnil Gorakh , Samarin Azin Mohagheghi , Samarin Azadeh Mohagheghi , Danielsen Marianne , Møller Hanne Søndergård , Policar Tomáš , Linhart Otomar , Dalsgaard Trine Kastrup
Published: June 2021

Aging is the most critical factor that influences the quality of post-ovulatory oocytes. Age-related molecular pathways remain poorly understood in fish oocytes. In this study, we examined the effect of oocyte aging on specific histone acetylation in common carp Cyprinus carpio. The capacity to progress to the larval stage in oocytes that were aged for 28 h in vivo and in vitro was evaluated. Global histone modifications and specific histone acetylation (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) were investigated during oocyte aging. Furthermore, the activity of histone acetyltransferase (HAT) was assessed in fresh and aged oocytes. Global histone modifications did not exhibit significant alterations during 8 h of oocyte aging. Among the selected modifications, H4K12ac increased significantly at 28 h post-stripping (HPS). Although not significantly different, HAT activity exhibited an upward trend during oocyte aging. Results of our current study indicate that aging of common carp oocytes for 12 h results in complete loss of egg viability rates without any consequence in global and specific histone modifications. However, aging oocytes for 28 h led to increased H4K12ac. Thus, histone acetylation modification as a crucial epigenetic mediator may be associated with age-related defects, particularly in oocytes of a more advanced age.
Self-Assembling Systems Based on Pillar[5]arenes and Surfactants for Encapsulation of Diagnostic Dye DAPI
Authors: Nazarova Anastasia , Khannanov Arthur , Boldyrev Artur , Yakimova Luidmila , Stoikov Ivan
Published: June 2021

In this paper, we report the development of the novel self-assembling systems based on oppositely charged Pillar[5]arenes and surfactants for encapsulation of diagnostic dye DAPI. For this purpose, the aggregation behavior of synthesized macrocycles and surfactants in the presence of Pillar[5]arenes functionalized by carboxy and ammonium terminal groups was studied. It has been demonstrated that by varying the molar ratio in Pillar[5]arene-surfactant systems, it is possible to obtain various types of supramolecular systems: host–guest complexes at equimolar ratio of Pillar[5]arene-surfactant and interpolyelectrolyte complexes (IPECs) are self-assembled materials formed in aqueous medium by two oppositely charged polyelectrolytes (macrocycle and surfactant micelles). It has been suggested that interaction of Pillar[5]arenes with surfactants is predominantly driven by cooperative electrostatic interactions. Synthesized stoichiometric and non-stoichiometric IPECs specifically interact with DAPI. UV-vis, luminescent spectroscopy and molecular docking data show the structural feature of dye-loaded IPEC and key role of the electrostatic, π–π-stacking, cation–π interactions in their formation. Such a strategy for the design of supramolecular Pillar[5]arene-surfactant systems will lead to a synergistic interaction of the two components and will allow specific interaction with the third component (drug or fluorescent tag), which will certainly be in demand in pharmaceuticals and biomedical diagnostics.
MRTF: Basic Biology and Role in Kidney Disease
Authors: Miranda Maria Zena , Lichner Zsuzsanna , Szászi Katalin , Kapus András
Published: June 2021

A lesser known but crucially important downstream effect of Rho family GTPases is the regulation of gene expression. This major role is mediated via the cytoskeleton, the organization of which dictates the nucleocytoplasmic shuttling of a set of transcription factors. Central among these is myocardin-related transcription factor (MRTF), which upon actin polymerization translocates to the nucleus and binds to its cognate partner, serum response factor (SRF). The MRTF/SRF complex then drives a large cohort of genes involved in cytoskeleton remodeling, contractility, extracellular matrix organization and many other processes. Accordingly, MRTF, activated by a variety of mechanical and chemical stimuli, affects a plethora of functions with physiological and pathological relevance. These include cell motility, development, metabolism and thus metastasis formation, inflammatory responses and—predominantly-organ fibrosis. The aim of this review is twofold: to provide an up-to-date summary about the basic biology and regulation of this versatile transcriptional coactivator; and to highlight its principal involvement in the pathobiology of kidney disease. Acting through both direct transcriptional and epigenetic mechanisms, MRTF plays a key (yet not fully appreciated) role in the induction of a profibrotic epithelial phenotype (PEP) as well as in fibroblast-myofibroblast transition, prime pathomechanisms in chronic kidney disease and renal fibrosis.
Novel Chemically Modified Curcumin (CMC) Analogs Exhibit Anti-Melanogenic Activity in Primary Human Melanocytes
Authors: Goenka Shilpi , Simon Sanford R.
Published: June 2021

Hyperpigmentation is a dermatological condition characterized by the overaccumulation and/or oversecretion of melanin pigment. The efficacy of curcumin as an anti-melanogenic therapeutic has been recognized, but the poor stability and solubility that have limited its use have inspired the synthesis of novel curcumin analogs. We have previously reported on comparisons of the anti-melanogenic activity of four novel chemically modified curcumin (CMC) analogs, CMC2.14, CMC2.5, CMC2.23 and CMC2.24, with that of parent curcumin (PC), using a B16F10 mouse melanoma cell model, and we have investigated mechanisms of inhibition. In the current study, we have extended our findings using normal human melanocytes from a darkly pigmented donor (HEMn-DP) and we have begun to study aspects of melanosome export to human keratinocytes. Our results showed that all the CMCs downregulated the protein levels of melanogenic paracrine mediators, endothelin-1 (ET-1) and adrenomedullin (ADM) in HaCaT cells and suppressed the phagocytosis of FluoSphere beads that are considered to be melanosome mimics. All the three CMCs were similarly potent (except CMC2.14, which was highly cytotoxic) in inhibiting melanin production; furthermore, they suppressed dendricity in HEMn-DP cells. CMC2.24 and CMC2.23 robustly suppressed cellular tyrosinase activity but did not alter tyrosinase protein levels, while CMC2.5 did not suppress tyrosinase activity but significantly downregulated tyrosinase protein levels, indicative of a distinctive mode of action for the two structurally related CMCs. Moreover, HEMn-DP cells treated with CMC2.24 or CMC2.23 partially recovered their suppressed tyrosinase activity after cessation of the treatment. All the three CMCs were nontoxic to human dermal fibroblasts while PC was highly cytotoxic. Our results provide a proof-of-principle for the novel use of the CMCs for skin depigmentation, since at low concentrations, ranging from 5 to 25 µM, the CMCs (CMC2.24, CMC2.23 and CMC2.5) were more potent anti-melanogenic agents than PC and tetrahydrocurcumin (THC), both of which were ineffective at melanogenesis at similar doses, as tested in HEMn-DP cells (with PC being highly toxic in dermal fibroblasts and keratinocytes). Further studies to evaluate the efficacy of CMCs in human skin tissue and in vivo studies are warranted.