bluuebunny/crossref_metadata_2025 huggingface dataset:
KIRSAL ALANDA KADIN GİRİŞİMCİLİĞİ: BATI AKDENİZ BÖLGESİNDE TKDK’DAN HİBE ALAN KADINLAR ÜZERİNDE BİR ARAŞTIRMA
Authors: ACAR Zeliha Merve , ÇAĞLAR Nedret
Published: December 2019

Kırsal alanlarda aile içerisinde aktif olarak çalışan ancak emeğinin karşılığını al(a)mayan, çalışması resmi kayıtlara yansımayan kadınların kendi işini kurmaları ve iş sahibi olmaları çok önemlidir. Son yıllarda bazı kamu kurumları ya da çeşitli kuruluşlar bu konuya ilgisiz kalmamış AB’ye uyumun da bir gereği olarak kadın girişimciliğine çeşitli şekillerde desteklerde bulunmaya başlamışlardır. Bu kuruluşlardan biri olan Tarım ve Kırsal Kalkınmayı Destekleme Kurumu (TKDK); kırsal alanlarda yaşayan kadınlara hibeler vererek onları yatırım yapmaya ve girişimciliğe özendirmeye çalışmaktadır. Bu çalışmanın da temel amacı; TKDK’dan hibe alarak kırsal alanda girişimciliğe yönelen kadınların yapmakta oldukları faaliyetlerin, öncelikle kadının kendisine ve ailesine daha sonra ise bulunduğu yöreye ve kırsal kalkınmaya etkisinin incelenmesidir. Çalışmada nitel araştırma teknikleri kullanılmıştır. Elde edilen bulgulara göre; hibe ile girişimciliğe yönelen kadınların kurmuş oldukları işletmeleri ile kişisel imaj ve özgüvenlerinin olumlu yönde geliştiği, girişimciliğin maddi imkânlarını arttırdığı, kırsal alanlara ekonomik, sosyal ve istihdam imkânları gibi daha birçok yönden katkıda bulundukları gözlemlenmiştir.
Factors Affecting Physical Self-Care Among Patients with Tuberculosis
Authors: Dwidiyanti Meidiana , Sari Sri Padma , Wijayanti Diyan Yuli , Ningsih Hasanah Eka Wahyu
Published: December 2019

Background physical self-care is necessary to gain patient involvement in health care in order to improve health outcome among tuberculosis patients. However, Factors related to support of physical self-care among patient tuberculosis under treatment was very rarely considered. This study aimed to identify the correlation between coping strategy, self-care management process and family well-being as factors associated with physical self-care. Method: A correlation study was conducted among forty-five of tuberculosis patients using purposive sampling. Data were collected within follow up session after implementing self-management support intervention by using physical self-care, coping strategy, self-care management process and family well-being questionnaire. Pearson test was used to analyze the data. Result: There were a significant association between self-care management process (r= 0.590; p=0.000), family well-being (children) (r=0.331; p=0.000) and physical self-care. However, there were no relationship between family well-being (parent) (p=0.789), coping strategy (p=0.874) and physical self-care among tuberculosis patient. Conclusion: Self-care management process and family well-being on children are essential factors to improve physical self-care among tuberculosis patient. Therefore, intervention which integrate those factors are important to enhance physical self-care among patients with tuberculosis
Effects of ‘STOP’ Mindfulness on Decreasing Cortisol in Primigravida Mothers
Authors: Anggorowati Anggorowati , Munawaroh Siti , Dwidiyanti Meidiana
Published: December 2019

Background: Stress in pregnant women may give different effects on the development of pregnancy. Mindfulness is an intervention which can be practiced independently by primigravida mothers for stress management. Stress is indicated by a biological parameter of cortisol levels.   

Purpose: This study aimed to analyze the effects of mindfulness on decreasing cortisol levels in primigravida mothers. 

Methods: This study used a pretest-posttest quasy experimental design with a control group. The samples were 30 primigravida mothers who experienced stress in the area of public health centers in Semarang city. A consecutive sampling technique was used to select the samples. Mindfulness with STOP technique (Stop-Take a breath-Observe-Proceed) was given for four meetings for 60 minutes each. Before and after the implementation of mindfulness, 3 ml of blood was taken from the samples for the cortisol examination. Data were analyzed using a paired t-test.

Results: The results showed that the majority of women (46.7%) were in the second trimester of pregnancy, and aged 20-35 years old (86.7%). The mean of cortisol level before and after the intervention was 180.60 and 152.50, respectively. There was a decrease in the level of cortisol after the implementation of mindfulness with a p-value of 0.000 (? <0.05).
Conclusion: Mindfulness is effective in decreasing cortisol levels in primigravida mothers. Mindfulness can be used as an intervention to increase the self-reliance of pregnant women for stress management.
NOVEL ATHEIS KARYA ACHDIAT KARTA MIHARDJA SEBAGAI MATERI PEMBELAJARAN SASTRA : ANALISIS PSIKOLOGI SASTRA DAN NILAI PENDIDIKAN
Authors: Saktiyono Haryo Seto , Waluyo Herman J , Hastuti Sri
Published: December 2019

The objectives of this research is to describe: (1) id psychology on the main character in Atheis novel by Achdiat Karta Mihardja; (2) ego psychology on the main character in the Atheis novel; (3) super-ego psychology on the main character in the Atheis novel; (4) the educational value contained in the Atheis novel; (5) its relevance as teaching material in learning literature appreciation in high school.This research uses descriptive qualitative method with psychology approach of literature. This research uses descriptive qualitative method with psychology approach of literature. The data obtained by researchers coming from Atheis novel, interviews with writers, lecturers of Indonesian language studies program, teachers and students. The validity of data is obtained through triangulation theory. Data were analyzed using analysis techniques interactive.The conclusions of this study were (1) the psychology of the main character in the Atheis novel by Achdiat Karta Mihardja is a Hasan character who suffers from psychiatric disorder that is the uncertainty of choosing religious belief (2) there are eleven grades of character traits contained in Atheis novel, they are religious values, tolerance, hard work, social caring, curiosity, friendship, responsibility, self-reliance, peace, discipline, like to read (3) Atheis novel is relevant to the literary learning material in senior high school because it contains many moral messages and good character education value for students.
PEMBELAJARAN BAHASA INDONESIA BERBASIS KURIKULUM 2013 ( STUDI KASUS PEMBELAJARAN TEKS ULASAN CERPEN DI KELAS VIII SMP NEGERI 2 NGAWI )
Authors: Pinasti Intan Indria , Rohmadi Muhammad , Rakhmawati Ani
Published: December 2019

This research aimed to describe and explain (1) The planning of Bahasa Indonesia subject based on 2013 curriculum; (2) The learning process of Bahasa Indonesia subject based on curriculum 2013; (3) The problems faced by the teacher in the learning process of Bahasa Indonesia subject based on 2013 curriculum; (4) The solutions to overcome the problems during the learning process of Bahasa Indonesia subject based on 2013 curriculum. This research was a case-study. It carefully captured and observed the learning process of Bahasa Indonesia subject based on the planning, the acting, the result, the problems, and the solutions to overcome the problemsThe results of the research were descriptions of (1) The planning of Bahasa Indonesia subject based on 2013 curriculum; (2) That learning process of Bahasa Indonesia subject based on curriculum 2013; (3) The problems faced by the teacher in the learning process of Bahasa Indonesia subject based on 2013 curriculum; (4) The solutions to overcome the problems during the learning process of Bahasa Indonesia subject based on 2013 curriculum.
KAJIAN ANTROPOLOGI SASTRA DAN NILAI PENDIDIKAN DALAM CERITA RAKYAT KALANTIKA SERTA RELEVANSINYA SEBAGAI BAHAN AJAR DI SMP
Authors: Maulidiah Nurfitriana , Suyitno Suyitno , Mulyono Slamet
Published: December 2019

This research is proposed to describe and explain; (1) the themes in folktale Kalantika, (2) the cultural features in activities of folktale’s figure Kalantika, (3) the cultural result in folktale Kalantika, (4) the education value in set of folktale Kalantika,(5)  the relevance of set of folktale Kalantika with learning in Junior High School. This research used descriptive qualitative method with literary anthropology approach. Sampling technique that used in this research is purposive sampling. Collecting data technique use document analysis and interview. To validity the data, it uses triangulation of source. Data analysis technique in this research uses interactive analysis techniques. The results of this research shows that: (1) the themes of a set of folktale Kalantika were ideas about the human view of universe and knowledge system; (2) the cultural characteristic in activities of folktale’s figure Kalantika were activities about equipment of human life, livelihood system, and knowledge system; (3) the cultural result of set of folktale Kalantika that can be find are the cultural result in the form of livelihood system and economy system, and knowledge system also; (4) education value that can be find are moral value, social value, religion value, and culture value; (5) Kalantika can be some teaching materials in literary learning of Junior High School.
KESANTUNAN TINDAKTUTUR DIREKTIF DALAM NOVEL TERJEMAHAN HARRY POTTER TAHUN 1 DAN 2 KARYA J. K. ROWLING SERTA RELEVANSINYA SEBAGAI BAHAN AJAR DI SMP
Authors: Sriyatmoko Agus , Purwadi Purwadi , Suhita Raheni
Published: December 2019

The purpose of this study is to describe (1) the form of directive speech acts in JK Rowling's Harry Potter novels year 1 and 2, (2) the form of language politeness, (3) the relevance of the Harry Potter novelsyear 1 and 2 in Indonesian language learning to students in middle school. The data collection technique used in this study was purposive sampling and data analysis used an interactive analysis model. The results of this study indicate that the form of directive speech acts and compliance with the principles of politeness contained in the Harry Potter novels year 1 and 2 by J. Rowling are  as follows: (1) speech acts; requests, questions, rules, prohibitions, granting permits, and directive forms of counseling which numbered 419 data; (2) compliance with the principles of teacher and student politeness during the learning process is found as many as 118 data; (3) Harry Potter novels year 1 and 2 by J. K. Rowling are related to literary learning in junior high schools.
IMPLEMENTASI KURIKULUM 2013 EDISI REVISI DALAM PEMBELAJARAN MEMBACA PUISI (STUDI KASUS DI KELAS X IPS 1 SMA NEGERI 1 KARANGANYAR)
Authors: Sari Nofi Dian , Setiawan Budhi , Mujiyanto Yant
Published: December 2019

This research is a qualitative research with case study approach. The population in this study were class X students of SMA Negeri 1 Karanganyar. The research sample was students of class X IPS 1 with purposive sampling sampling technique. Data sources include events, informants, and documents. Data collection techniques such as observation, interview, and document analysis. The data validity test consists of triangulation of data sources, method triangulation, and informant review. Data analysis technique is interactive model of analysis. The results of this study include: (1) teachers' perceptions of the implementation of the Curriculum 2013 Revised Edition on learning to read poetry is good; (2) instructional planning is prepared in accordance with Permendikbud Number 22 Year 2016; (3) the implementation of reading poetry learning works well; (4) assessment of learning has not been done effectively; (5) obstacles in the form of: (a) limited face-to-face learning time in semester 2; (b) the implementation of a less effective learning assessment; (c) not all students have the talent to read poetry and students find it difficult to find the atmosphere of poetry; (d) subjectivity in assessing literary learning; (6) efforts in the form of: (a) providing structured and unstructured tasks; (b) the use of tasks in group discussion so that the assessment of poetry reading can still be carried out in its entirety; (c) students are required to frequently see references to reading poetry from youtube; d) making assessment criteria.
ANALISIS STILISTIKA NOVEL DARI HARI KE HARI KARYA MAHBUB DJUNAIDI DAN RELEVANSINYA SEBAGAI MATERI AJAR BAHASA INDONESIA DI SEKOLAH MENENGAH ATAS
Authors: Cahyono Nur , Andayani Andayani , Mujiyanto Yant
Published: December 2019

The aims of this research are to describe diction (word choise, figure of a speech, imagery of Dari Hari Ke Hari’s novel by Mahbub Djunaidi, and the relevance of the results of studies as Bahasa Indonesia teaching material for the Senior High School. This study is a qualitative descriptive with content analysis method. The result showed that. First, the use of diction (choice words) includes concrete words, connotative words, word greetings or self-names, vulgar words, words of absorption, regional vocabulary, and words with natural reality. Second, the use of language style (Figure of a speech) includes simile, metaphor, personification, depersonification, allegory, antithesis, pleonasme, periprasis, anticipation, epanortosis, satire, paradox, climax, anti-climax, allusion, atonomation, eroteris, asyndeton, polisedenton, epizeukis, anaphora, and epystrofa. Third, the use of imagery includes visual imagery, motion imagery, olfactory imagery, tactical imagery, and auditory images. Fourth, Dari Hari Ke Hari’s novel can be used as a teaching material in high school literature class XII curriculum of 2013, ie at base competence understand the structure and rules of novel text.
KESALAHAN BERBAHASA PADA KARANGAN EKSPOSISI SISWA SEKOLAH MENENGAH ATAS: BENTUK, FAKTOR PENYEBAB, DAN UPAYA MENGATASI
Authors: Kismawati Utari , Sumarwati Sumarwati , Wardhani Nugraheni Eko
Published: December 2019

The research population was all tenth grade Social students of Sekolah Menengah Atas Negeri 6 Surakarta. The research method used was qualitative method using descriptive-qualitative approach. The participants of the research were one hundred thirty (130) Social students’ works. The sampling technique used was purposive sampling. Data collection techniques were content analysis and in-depth interviewing. Data analyzing used was interactive analysis model. The followings are the conclusions of the research. First, three types of language errors in students’ exposition text are sentence error, diction error, and spelling error. Second, the most detected type of error is spelling error in which the use of comma has the highest percentage. Third, the casual factors of language error were as follow: the students know less about writing principles; the students have less motivation to read; the students tend to emphasize the idea rather than writing principle; the students tend to be lazy to do editing before submitting their works; the class condition is not supportive; and the students are demanded to write structurally correct. Fourth, some efforts done to minimize language error: teachers give clarification immediately when they notice language error; cross-reading among students before submitting their works; and more writing activities in class.
KAJIAN STILISTIKA DAN NILAI PENDIDIKAN KARAKTER DALAM LIRIK LAGU PADA ALBUM DON’T MAKE ME SAD KARYA LETTO SERTA RELEVANSINYA SEBAGAI BAHAN AJAR SASTRA DI SEKOLAH MENENGAH ATAS
Authors: Abdillah Fahmi Diannafi , Rakhmawati Ani , Anindyarini Atikah
Published: December 2019

The aims of this research are to describe: (1) the use of diction in the lyrics of the song album Don’t Make Me Sad by Letto; (2) the existence of imagery in the lyrics of the song Don’t Make Me Sad by Letto; (3) the dominant style of language in the lyrics of the song album Don’t Make Me Sad by Letto; (4) the value of character education in the lyrics of the song album Don’t’ Make Me Sad by Letto; and (5) the relevance with the teaching materials of literature for senior high school students. This research is descriptive qualitative research using content analysis method. The source of data in this research is the lyrics of the song album Don’t Make Me Sad by Letto and the result of interview with informants. The technique of taking the sample used in this research was purposive sampling. The technique of collecting the data in this research was data analysis and interview with the informants. This research uses source triangulation and method triangulation to validate the data. The researcher uses interactive analysis method in this research. 
PERJUANGAN TOKOH PEREMPUAN JAWA DALAM NOVEL THE CHRONICLE OF KARTINI KARYA WIWID PRASETYO (KAJIAN FEMINISME DAN NILAI PENDIDIKAN KARAKTER)
Authors: Muslimah Nur Dwiana , Suyitno Suyitno , Purwadi Purwadi
Published: December 2019

This study aims to describe: (1) gender inequalities experienced by Javanese women in the novel The Chronicle OF Kartini; (2) the emancipation of Javanese women in the novel The Chronicle OF Kartini; (3) the value of character education contained in the novel The Chronicle OF Kartini. The research was conducted in October 2017 until March 2018. This research is a qualitative research using feminism approach. The source of this research data is a novel entitled The Chronicle OF Kartini by Wiwid Prasetyo totaling 415 pages. Sampling technique using purposive sampling. Data collection techniques used are document analysis. Data validation techniques use triangulation theory. Data analysis technique used is interactive analysis model, including data collection, data reduction, data presentation, and conclusion. The results of this study indicate that: (1) gender inequalities experienced by Javanese women in the form of maginalization, subordination, stereotype, and violence; (2) the emancipation of women contained in the novel The Chronicle OF Kartini in the form of freedom of choice for women, the struggle and resistance of women, and the independence and ferocity of women: (3) in the novel The Chronicle Of Kartini contains 18 types of representation of the value of character education.
MEMBUAT PETA PIKIRAN PADA KEGIATAN PRABACA UNTUK MENGIDENTIFIKASI IDE POKOK DAN MENYIMPULKAN ISI TEKS BERITA MELALUI COLLABORATIVE ACTION RESEARCH
Authors: Sumarwati Sumarwati , Martabatul Siti
Published: December 2019

One of the obstacles in developing reading skills in students is that they are not trained in reading activities for specific purposes. Therefore, students read by giving equal attention to all parts of the reading. Thi study aims to improve the reading ability through thue use of  mind mapping. This study was an classroom  action research and conducted  in three cycles. The participants were students class 8D and Indonesian Language  teacher of SMP Negeri 8 Surakarta. The result showed that there was an improvement in the percentace of  formulating  the mind map,   reading activity and  ability. The effective learning procedure was as follows. The students surveyed the text, identified the main idea of the text with questions word 5W + 1 H, formulated mind map,  memorized the main idea of mind map, read the text intensively that they could use their maps as a review of information gained,  stated the conclusion about ideas in the mind map, and answered the questions (take a test.  The conclusion of this study is that the main ideas identified in the pre-reading activities to be developed into a mind band, not only allows the reading activities with a specific purpose, but also helps students to focus attention on the parts of the text.
17α-Methyltestosterone can induce masculinisation and sterilisation in amur carp Cyprinus rubrofuscus Lacepede 1803
Authors: Suma K.S. , Basavaraja N. , Manjappa N.
Published: December 2019

To find a suitable solution to the problem of early sexual maturation and unwanted reproduction during grow-out, the present study was aimed at production of sterile or monosex population of Amur carp through endocrine manipulation. In this investigation, 24-day-old Amur carp fry were fed with diets containing 17α-methyltestosterone (17α-MT) at dosages of 200-1000 ppm for 30 d. While 17α-MT incorporation at 400 ppmresulted in complete masculinization, 600 ppm induced 100% sterilization. 17α-MT at 200 ppm induced the production of intersex and sterile fish. At 800 ppm, it produced 8.33% male, 8.33% female and 83.34% sterile fish, whereas 1000 ppm yielded 63.63% male, f9.10% female and 27.27% sterile fish. After 168 d post-treatment rearing, increase in fish weight was 16.01- 233.15%, over control. Loss of weight due to evisceration was 8.75-12.43% in control, while it was 7.31-9.78% in sterile fish, indicating the availability of more edible meat in the latter. MT-treated carp showed a slight variation in survival, gonadosomatic index (GSI), hepatosomatic index (HSI), viscerosomatic index (VSI) and condition factor. The histology of gonads revealed normal development of ovary and testis in control fish, but in 17α-MT treated fish the gonadal development was either normal or abnormal in male and slightly suppressed in female. Intersex fish had both ovarian and testicular tissues, while sterile fish possessed mostly connective tissue.
The complete mitochondrial genome and phylogeny of the green chromide, Etroplus suratensis (Bloch, 1790) from Vembanad Lake, Kerala, south India
Authors: Sebastian Wilson , Sukumaran Sandhya , A Gopalakrishnan
Published: December 2019

The green chromide Etroplus suratensis (Bloch, 1790), is a cichlid species which forms an economically valuable food fish and a preferred candidate for brackishwater aquaculture in India. The complete mitogenome of E. suratensis collected from Vembanad Lake, Kerala, India has been characterised in the present study. The entire mitogenome was PCR amplified as contiguous, overlapping segments and sequenced. The assembled mitogenome of E. suratensis is 16456 bp circle, contained the 37 mitochondrial structural genes, two ribosomal RNA genes (12S rRNA and 16S rRNA), 22 transfer RNA (tRNA) genes, 13 protein-coding genes and 1 non-coding control region/D-loop, with the gene order identical to vertebrates. In the phylogenetic analysis, E. suratensis is clustered with other Indo-Sri Lankan taxa. Among cichlids, the groups from South America and Africa are monophyletic in origin. The mitogenomic information generated in this study will be valuable for further studies on evolution, taxonomy, conservation, environmental adaptation and selective breeding of this species having aquaculture, ornamental and evolutionary importance.
Spatial variations in the population characteristics of sea cucumber resources in Gulf of Mannar and Palk Bay, south-east coast of India
Authors: Asha P.S. , Ranjith L. , Vivekanandan E. , Johnson B. , Subin C.S. , Sheik Mohamed M.
Published: December 2019

The lack of data on population abundance, biomass and basic biological parameters of most of the sea cucumber species from Indian waters is a fundamental barrier to impose suitable management measures on these resources. A study was conducted to ascertain the sea cucumber population characteristics in two geographically distinct ecosystems (Gulf of Mannar and Palk Bay) along the south-east coast of India. The stock status of sea cucumber population was assessed both by trawl and dive surveys following standard methods on a random basis. Comparatively, a higher average density of total sea cucumbers was estimated in the Gulf of Mannar (3853±152.3 nos. ha-1) than in Palk Bay (2428.5±504.6 nos. ha-1). The species diversity, average length and weight of individual species were also higher in the Gulf of Mannar. The present study indicated significant variation in status and structure of sea cucumber resources in these two ecosystems. Hence the study warrants the emphasis of ecosystem-based management in formulating conservation measures of these resources in Indian waters.
Association pattern between dimensions of fish and otolith to expedite morphometric variations of three geographically isolated stocks of Tenualosa ilisha (Hamilton, 1822) from diverse ecosystems
Authors: Borah Simanku , Vaisakh G. , Jaiswar A.K. , Bhattacharjya B.K. , Sahoo A. K. , Deshmukhe G. , Behera B.K. , Meena D.K. , Raman R.K. , Das B.K.
Published: December 2019

An attempt was made to establish association pattern between dimension of fish and otolith to expedite morphometric variations of geographically isolated stocks of Tenualosa ilisha (Hamilton, 1822), from three diverse ecosystems; viz., an estuarine (brackish water), a riverine (freshwater) and a lacustrine (freshwater) ecosystem. Sampling was carried out during the period from September 2017 to February 2019 and a total of 196 otoliths were sampled from Narmada Estuary (n=69), Brahmaputra River (n=75) and Ukai Reservoir (n=52) of India. Mean otolith length recorded was 3.55±0.14 mm, 5.18±0.09 mm and 3.85±0.07 mm for samples collected from Narmada Estuary, Brahmaputra River and Ukai Reservoir respectively. Otolith length-weight relationship (OLWR) from three ecosystems showed negative allometric b values ranging from 1.9046-2.5048, with significant difference between Narmada Estuary and Brahmaputra River (p<0.05). Power regression analysis of otolith dimensions (length and width) to total length of fish (TL) and otolith length (OL) to total weight of fish (TW) showed that data fitted well to the model as evident from R2 values (>0.6); except for relationship between otolith width (OB) and TL in samples from Brahmaputra River (R2=0.459). Present study provides first-hand information on association pattern of dimension of fish body and otolith of T. ilisha which can be used in geographical mapping of stock profile of the species.
Vitamin D receptor genetic polymorphisms are associated with oral lichen planus susceptibility in a Chinese Han population
Authors: Shen Hong , Liu Qinglan , Huang Peng , Fan Haozhi , Zang Feng , Liu Mei , Zhuo Lingyun , Wu Jingjing , Wu Guoying , Yu Rongbin , Yang Jianrong
Published: December 2019

Background: Vitamin D receptor (VDR) is involved in multiple immune-mediated disorders including oral lichen planus (OLP). This study investigated the association between VDR gene polymorphisms and the risk of OLP. Methods: In total, 177 OLP patients and 207 healthy participants were recruited from the Affiliated Hospital of Stomatology, Nanjing Medical University. Eight single nucleotide polymorphisms (SNPs: rs731236, rs739837, rs757343, rs2107301, rs2239185, rs7975232, rs11574129 and rs11568820) in the VDR gene were selected and genotyped. Results: The results showed that OLP risk was increased in subjects with the rs2239185 TT genotype (Recessive model: adjusted OR = 2.68, 95% CI = 1.28-5.62, P = 0.009) and rs7975232 CC genotype (Recessive model: adjusted OR = 2.25, 95% CI = 1.10-4.58, P = 0.026). Moreover, rs2239185 and rs7975232 (P < 0.01) showed significant cumulative effects on OLP risk...Haplotype analysis showed that the CC haplotype (rs2239185-rs7975232) was associated with an increased risk of OLP (OR = 3.11, 95% CI = 1.42-6.83, P = 0.005), compared with the AC haplotype. Conclusion: the rs2239185 and rs7975232 variants of VDR may influence OLP susceptibility, and VDR gene polymorphisms may be candidate susceptibility regions for OLP in a Chinese Han population.
Factors Associated With Dialysis Withdrawal In Dialysis Patients
Authors: Qazi Hammad , Chen Helen , Varga Peter , Chaurasia Ashok , Ebad Mahsa
Published: December 2019

Background: Research on the factors associated with dialysis withdrawal in dialysis patients has been limited. Authors have used different definitions for dialysis withdrawal, resulting in inconsistent findings. The objective of this study was to determine the factors associated with dialysis withdrawal in dialysis patients.Methods: This retrospective study extracted patient information from the electronic renal patient management systems at the Grand River Hospital. A total of 723 patients who initiated renal dialysis therapy (> 30 days of duration) in the renal dialysis program at Grand River Hospital (GRH), Ontario, during the period from 1st January 2012 to 30th September 2017 were consecutively included in the study. Logistic regression was used to determine the factors: age, sex, modality, comorbidities, the cause of primary renal disease, dialysis modality, and duration of dialysis-associated with dialysis withdrawal. Dialysis withdrawal was defined as “patient declined further treatment or voluntary withdrawal from the dialysis program”.Results: The mean age of the sample was 64.86 ± 14.89 years, and 62.8% (n = 454) were males. The prevalence of dialysis withdrawal was 9.41% (n = 68). The logistic regression model showed that factors associated with dialysis withdrawal were as follows: cardiac disease [Adjusted Odds Ratio (AOR)= 1.921; 95% CI= 1.126–3.278], hypertension [AOR = 5.711; 95% CI = 1.322- 24.676], dementia [AOR = 3.042; 95% CI = 1.325–6.983], age [AOR = 1.035; 95% CI = 1.012– 1.058] and duration of dialysis [AOR = 0.999; 95% CI = 0.999–1.00].Conclusion: In this study we show that age, cardiac disease, hypertension, and dementia are significant predictors related to dialysis withdrawal. The findings might help in identifying patients who are more likely to withdraw from dialysis at the start of dialysis. Future researchers and nephrologists should design and conduct intervention studies focusing on strategies controlling the severity of comorbidities (cardiac disease and hypertension), regular assessment and monitoring of the progression of dementia, and other dialysis program changes to help patients make more informed decisions regarding dialysis withdrawal. Keywords: dialysis, dialysis withdrawal, maintenance dialysis, hemodialysis, peritoneal dialysis, dementia.
Competitive endogenous RNA (ceRNA) regulation network of lncRNAs, miRNAs, and mRNAs in Wilms tumour
Authors: tang fucai , Lu zechao , wang jiamin , Li zhibiao , Wu weijia , Duan Haifeng , He zhaohui
Published: December 2019

Background: Competitive endogenous RNAs (ceRNAs) have revealed a new mechanism of interaction between RNAs. However, an understanding of the ceRNA regulatory network in Wilms tumour (WT) remains limited.Methods: The expression profiles of mRNAs, miRNAs and lncRNAs in Wilms tumour samples and normal samples were obtained from the Therapeutically Applicable Research to Generate Effective Treatment (TARGET) database. The EdgeR package was employed to identify differentially expressed lncRNAs, miRNAs and mRNAs. Functional enrichment analyses via the ClusterProfile R package were performed, and the lncRNA–miRNA–mRNA interaction ceRNA network was established in Cytoscape. Subsequently, the correlation between the ceRNA network and overall survival was analysed.Results: A total of 2,037 lncRNAs, 154 miRNAs and 3,609 mRNAs were identified as differentially expressed RNAs in Wilms tumour. Of those, 205 lncRNAs, 26 miRNAs and 143 mRNAs were included in the ceRNA regulatory network. The results of Gene Ontology (GO) analysis revealed that the differentially expressed genes (DEGs) were mainly enriched in terms related to response to mechanical stimuli, transcription factor complexes, and transcription factor activity (related to RNA polymerase II proximal promoter sequence-specific DNA binding). The results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the DEGs were mainly enriched in pathways related to the cell cycle. The survival analysis results showed that 16 out of the 205 lncRNAs, 1 out of 26 miRNAs and 5 out of 143 mRNAs were associated with overall survival in Wilms tumour patients (P < 0.05).Conclusions: CeRNA networks play an important role in Wilms tumour. This finding might provide effective, novel insights for further understanding the mechanisms underlying Wilms tumour.
CD8-positive memory T cells in tumor-draining lymph nodes of patients with breast cancer
Authors: Vahidi Yasmin , Bagheri Mandana , Ghaderi Abbas , Faghih Zahra
Published: December 2019

Background: Human immunological memory is a hallmark of the adaptive immune system and plays an important role in the development of effective immune responses against tumors. In the present study, we aimed to determine the frequencies of CD8+ memory T cell subsets including stem memory T cells (TSCM) in tumor-draining lymph nodes of patients with breast cancer (BC).
Methods: Mononuclear cells were obtained from axillary lymph nodes of 52 untreated patients with BC and stained for CD8, CCR7, CD45RO, CD95 markers to detect different subtypes of memory cells in the CD8+ lymphocyte population. Data were acquired on four-color flow cytometry and analyzed with CellQuest Pro software.
Results: We observed that 47.65±2.66 of CD8+ lymphocytes expressed the CD45RO, a marker for memory T cells. Statistical analysis showed that the total frequency of central memory T cells (TCM) and their subset with low CD45RO expression was significantly higher in tumor-involved nodes compared to tumor-free ones (P=0.024 and P=0.017, respectively). The level of CD95 expression (based on mean fluorescence intensity) on the surface of TCM, their CD45ROhi and CD45ROlow subsets, and TSCM was higher in patients with stage II compared to those in stage I (P<0.05). In addition, the percentage of naive CD8+ T cells was significantly higher in tumor-involved lymph nodes compared to tumor-free ones (P=0.025).
Conclusions: Our data collectively indicate no significant differences in the frequencies of CD8+ lymphocytes or their memory subsets in tumor-draining lymph nodes of patients with BC. However, the frequencies of CD45low TCM along with naive CD8+ lymphocytes were higher in tumor-involved nodes, which suggests that after long-term exposure to the antigen, and despite the immune reaction in order to provide a pool of effective memory cells, memory cell differentiation is blocked in early-stage (CD45ROlow) due to tumor-derived suppressive factors. Identifying the molecular and cellular mechanisms behind this suppression can provide invaluable tools for adoptive T cell therapies in cancer.
Investigation of Potential Genetic Biomarkers and Molecular Mechanism of Ulcerative Colitis Utilizing Bioinformatics Analysis
Authors: Zhang Jiaqi , Wang Xue , Xu Lin , Zhang Zedan , Wang Fengyun , Tang Xudong
Published: December 2019

The authors have withdrawn this preprint from Research Square
Retrospective cohort study of trifluridine/tipiracil (TAS-102) plus bevacizumab versus trifluridine/tipiracil monotherapy for metastatic colorectal cancer
Authors: Kotani Daisuke , Kuboki Yasutoshi , Horasawa Satoshi , Kaneko Asumi , Nakamura Yoshiaki , Kawazoe Akihito , Bando Hideaki , Taniguchi Hiroya , Shitara Kohei , Kojima Takashi , Tsuji Akihito , Yoshino Takayuki
Published: December 2019

Background:
A previous phase I/II C-TASK FORCE study of trifluridine/tipiracil plus bevacizumab for patients with heavily pretreated metastatic colorectal cancer (mCRC) showed promising activity with an acceptable toxicity profile. This retrospective study aimed to investigate the safety and efficacy of trifluridine/tipiracil plus bevacizumab compared with trifluridine/tipiracil monotherapy in patients with heavily pretreated mCRC in clinical settings.
Methods:
Records of patients with mCRC refractory to standard therapies who initiated trifluridine/tipiracil plus bevacizumab from January 2016 to March 2018 or trifluridine/tipiracil monotherapy from June 2014 to December 2015 were retrospectively reviewed at our institution.
Results:
Totally, 60 patients received trifluridine/tipiracil plus bevacizumab and 66 received trifluridine/tipiracil monotherapy. All patients had previously received standard chemotherapy. Median progression-free survival (PFS) was 3.7 months [95% confidence interval (CI), 2.3−5.1] in the trifluridine/tipiracil plus bevacizumab group and 2.2 months (95% CI, 1.8−2.6) in the trifluridine/tipiracil monotherapy group [hazards ratio (HR) 0.69; 95% CI 0.48−0.99]. PFS rate at 16 weeks was 46.6% for the trifluridine/tipiracil plus bevacizumab group and 33.9% for the trifluridine/tipiracil monotherapy group. Although a relatively higher incidence of grade ≥3 neutropenia was observed in the trifluridine/tipiracil plus bevacizumab group than that in the other group (50.0% vs. 40.9%, p = 0.371), the incidence of febrile neutropenia was not high (3.3% vs. 7.8%, p = 0.444).
Conclusions:
In real-world settings, trifluridine/tipiracil plus bevacizumab prolonged PFS and helped achieve higher 16-week PFS rate compared with trifluridine/tipiracil monotherapy in patients with heavily pretreated mCRC with manageable toxicities.
Trial registration: Retrospectively registered
Knowledge-based genetic association study of hepatitis B virus related hepatocellular carcinoma
Authors: Jiang Deke , Deng Jiaen , Dong Changzheng , Ma Xiaopin , Xiao Qianyi , Zhou Bin , Yang Chou , Wei Lin , Conran Carly , Zheng S. Lilly , Ng Irene Oi-lin , Yu Long , Xu Jianfeng , Sham Pak C. , Qi Xiaolong , Hou Jinlin , Ji Yuan , Cao Guangwen , Li Miaoxin
Published: December 2019

Background : Recent genome-wide association studies (GWASs) have suggested several susceptibility loci of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) by statistical analysis at individual single-nucleotide polymorphisms (SNPs). However, these loci only explain a small fraction of HBV-related HCC heritability. In the present study, we aimed to identify additional susceptibility loci of HBV-related HCC using advanced knowledge-based analysis.Methods: We performed knowledge-based analysis (including gene- and gene-set-based association tests) on variant-level association p-values from two existing GWASs of HBV-related HCC. Five different types of gene-sets were collected for the association analysis. A number of SNPs within the gene prioritized by the knowledge-based association tests were selected to replicate genetic associations in an independent sample of 965 cases and 923 controls.Results: The gene-based association analysis detected four genes significantly or suggestively associated with HBV-related HCC risk: SLC39A8, GOLGA8M, SMIM31, and WHAMMP2. The gene-set-based association analysis prioritized two promising gene set for HCC, cell cycle G1/S transition and NOTCH1 intracellular domain regulates transcription. Within the gene sets, three promising candidate genes (CDC45, NCOR1 and KAT2A) were further prioritized for HCC. Among genes of liver-specific expression, multiple genes previously implicated in HCC were also highlighted. However, probably due to small sample size, none of the genes prioritized by the knowledge-based association analyses are successfully replicated in the independent sample. Conclusions: This comprehensive knowledge-based association mining study suggested several promising genes and gene-sets associated with HBV-related HCC risk. More experiments or larger samples are needed to validate their contribution to the pathogenic mechanism of HCC.
Evaluation of the "Licence, Master, Doctorate" reform at the Health Sciences faculty of the University of Lomé: strengths and weaknesses
Authors: TECLESSOU Julienne , KPELAO Essossinam , SAKA Bayaki
Published: December 2019

Introduction: The License, Master, and Doctorate (LMD) reform which structured high studies in three cycles was instituted since the Bologna declaration on 1999. To be conformed with international standards, the LMD system was instituted in university of Lomé in 2009 to foster flexible pathways between medical and paramedical training. The purpose of this study was to evaluate the strengths and weaknesses of the LMD reform since its introduction in medical school of Lomé.Method: It was an opinion survey conducted in 4 months in University of Lomé among the medical school’s teachers about strengths and weaknesses of LMD reform since its application. The strengths were defined as all facilities brought by LMD reform in organization of courses and practices, evaluations, new Information and Communication Technologies (ICTs) (internet, video projector, courses on line). The LMD weaknesses were defined as any problem generated by the LMD. Results: Seventy-six out of on 113 resident teachers of the medical school of Lomé had completed the questionnaires (67.2%). Mostly teachers (74) took that the introduction of LMD reform will make Lomé medical school on international standards. The availability of the video projectors was noted by 90.8% of the teachers and used for teaching in (82.9%). No course dispensed in medical school was online. The main strengths of LMD were: best student’s evaluation (33.3), the organization of training in units with credit (28.6), using new ICTs (23.8%). Many weaknesses of LMD reform were noted by teachers: the plethoric number of students (36.2%), the absence of intermediate diploma and pathways between studies (29.3). The Insufficiency of personnel resources and material was also mentioned. Conclusion: This study highlights that LMD reform needs adaptation to local realities and improvement to ensure that students will get good training in compliance with international standards.
Prevalence of Intestinal Parasitic Infections and Associated Factors Among Street Children in Jimma Town in 2019: A Cross Sectional Study
Authors: Siraj Sabit Zenu , Alemayehu Eshetu , Woldemichael Kifle
Published: December 2019

Background
Street child is any child whose age is less than 18 years for whom the street has become his or her habitual abode and/or source of livelihood, is inadequately protected, supervised or directed by responsible adults. In Ethiopia the health problems of street children are given poor attention in research. This problem is pronounced when it comes to intestinal parasitic infections . The aim of this study was to assess the prevalence of intestinal parasitic infections and associated factors among street children in Jimma town in the year 2019.
Methods and Materials: Community based cross sectional study was employed. Complete enumeration was used to include 312 street children. Pretested questionnaire was used to collect the data. Data was entered to Epidata version 3.1 and exported to SPSS version 20. Stool samples were examined by wet mount and formalin ether concentration techniques. Bivariable and multivariable logistic regression was used to identify factors associated with intestinal parasitic infection. Significance of association was decided by using the 95% confidence interval of AOR and P-value of ≤ 0.05 in the multivariable model.
Result: A total of 312 children of the street were involved in the study making the response rate 96.2%. The prevalence of intestinal parasitic infection was 66.7%. Untrimmed finger nails AOR=2.03;95%CI (1.02-4.06), eating street food AOR=2.24;95% CI (1.04-5.02), practice of swimming in unprotected water bodies AOR=2.5; 95% CI (1.24-5.04), not wearing shoes at the time of data collection AOR= 3.8;95% CI (1.8-8.2) and lacking knowledge of way of transmission of intestinal parasites AOR= 2.5; 95% CI (1.25- 5.0) were significantly associated with parasitic infections.
Conclusion and Recommendations: The prevalence of intestinal parasitic infections among street children in the study area was high and require integrated interventions to avert the problem. Factors like untrimmed finger nails, swimming practice, eating street foods, shoe wearing and lacking knowledge of ways of transmission of intestinal parasitic infections are associated with infection status. Measures has to be taken to curb the problem by including them in mass drug administration and targeted health education towards identified factors.
Key words : Street children, Intestinal parasites, Jimma
The effects of protective lung ventilation on regional cerebral oxygen saturation in intracranial tumor operation during dura opening：study protocol for a randomized controlled trial.
Authors: Li Jinlu , Wu Xuemei , Liu Hairui , Huang Ying , Liu Yueqin , Xie Hong , Dong Jun
Published: December 2019

Abstract Objective: To investigate the effects of protective lung ventilation on regional cerebral oxygen saturation during dura opening, that is from Ta (after dura opening) to Tb (before dura closing), in patients undergoing intracranial tumor surgery. Methods: This is a randomized, controlled trial which will be carried out at the second affiliated hospital of Soochow University. Fifty-four patients undergoing intracranial tumor surgery will be randomly allocated to the control group (C group) or the protective lung ventilation group (P group). In the C group, tidal volume (VT) will be set at 8 ml/kg of predicted body weight, but positive end-expiratory pressure (PEEP) and recruitment maneuvers will not be used. In the P group, VT will be set at 6 ml/kg of predicted body weight combined with individualized PEEP during dura opening, while in other periods of general anesthesia, VT will be set at 8 ml/kg of predicted body weight. Regional cerebral oxygen saturation (rSO2), partial pressure of oxygen (PaO2) and carbon dioxide (PaCO2), oxygenation index (OI), lactic acid level (Lac) in arterial blood, and mean arterial pressure (MAP) will be compared before anesthesia (T0), before dura opening (T1), after dura closing (T2) and 24 h after surgery (T3). Lung ultrasound scores (LUS) will be performed at T0 and T3. The degree of brain relaxation at T1 and T2 will be evaluated by the surgeon using the brain relaxation scale. Amount of vasoactive drugs used and blood loss will be recorded during surgery. The duration of operation and reoperation rate will be recorded. Discussion: This study aims to determine whether protective lung ventilation during dura opening can improve regional cerebral oxygen saturation and the state of pulmonary ventilation in patients undergoing intracranial tumor surgery, and to investigate whether this strategy does not affect the degree of brain tissue swelling and the reoperation rate after operation. If our results are positive, this study will show that protective lung ventilation during dura opening can be used effectively and safely in neurosurgical patients undergoing craniotomy for tumor resection. Trial registration: chictr.org.cn, ID: ChiCTR1900025632. Registered on 3 September 2019. Study protocol version 2.0. Keywords: Randomized controlled trial, Regional cerebral oxygen saturation, Lung ultrasound, Brain relaxation, Craniotomy
Positron emission tomography/computed tomography-guided percutaneous trans-pararectal space prostate biopsy for the diagnosis of prostate cancer: A retrospective study
Authors: Luo Nana , Ruan Dan , Pang Yi-zhen , Shang Qi-hang , Chen Hao-jun , Guo Wei , Hao Bing , Wu Hua , Sun Long
Published: December 2019

Background Biopsy is considered the gold-standard technique for prostate cancer diagnosis and is recommended in patients with a high clinical indication of prostate cancer. In this study, we aimed to determine the diagnostic efficacy of a novel positron emission tomography (PET)/computed tomography (CT)-guided percutaneous trans-pararectal space-based approach to targeted prostate biopsy.Methods PET/CT-guided percutaneous trans-pararectal space prostate biopsies were performed in 14 consecutive patients with indications of prostate cancer. Whole-body 18F-FDG PET/CT indicated the presence of 18F-fluorodeoxyglucose (FDG)-avid focal prostate lesions. Two tissue specimens were obtained from each patient. The final diagnoses were established based on the results of a histopathological analysis and clinical follow-up, and these findings were used to verify the diagnostic accuracy of 18F-FDG PET/CT for prostate cancer.Results The diagnostic accuracy of 18F-FDG PET/CT for prostate cancer was 81.8%. Further analyses of the two biopsied samples per patient led to confirmed histopathological and immunohistochemical diagnoses of prostate cancer in all 14 patients. Consequently, the success rate of PET/CT-guided percutaneous trans-pararectal space prostate-targeted biopsy for the diagnosis of prostate cancer was 100.0% (14/14). Regarding safety, the average duration of biopsy was 20 min, and no serious complications occurred.Conclusions PET/CT-guided percutaneous trans-pararectal space prostate biopsy may yield a new approach to targeted prostate biopsy for the diagnosis of prostate cancer. Moreover, this biopsy procedure can be performed safely without complications, and is more cost-effective than conventional trans-rectal and trans-perineal prostate biopsy methods.