bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined analysis of the transcriptome and metabolome provides insights into the fleshy stem expansion mechanism in stem lettuce
Authors: Huang Ying , Li Yanwen , Liu Zhenning , Chen Wanqin , Wang Yalin , Wang Xiaohua , Liu Yihua , Zheng Yangxia
Published: December 2022

As a stem variety of lettuce, the fleshy stem is the main product organ of stem lettuce. The molecular mechanism of fleshy stem expansion in stem lettuce is a complex biological process. In the study, the material accumulation, gene expression, and morphogenesis during fleshy stem expansion process were analyzed by the comparative analysis of metabolome, transcriptome and the anatomical studies. The anatomical studies showed that the occurrence and activity of vascular cambium mainly led to the development of fleshy stems; and the volume of pith cells gradually increased and arranged tightly during the expansion process. A total of 822 differential metabolites and 9,383 differentially expressed genes (DEGs) were identified by the metabolomics and transcriptomics analyses, respectively. These changes significantly enriched in sugar synthesis, glycolysis, and plant hormone anabolism. The expression profiles of genes in the sugar metabolic pathway gradually increased in fleshy stem expansion process. But the sucrose content was the highest in the early stage of fleshy stem expansion, other sugars such as fructose and glucose content increased during fleshy stem expansion process. Plant hormones, including IAA, GA, CTK, and JA, depicted important roles at different stem expansion stages. A total of 1,805 DEGs were identified as transcription factors, such as MYB, bHLH, and bZIP, indicating that these transcription factor families might regulate the fleshy stems expansion in lettuce. In addition, the expression patterns identified by qRT-PCR were consistent with the expression abundance identified by the transcriptome data. The important genes and metabolites identified in the lettuce fleshy stem expansion process will provide important information for the further molecular mechanism study of lettuce fleshy stem growth and development.
Fame and factions: A process-tracing analysis of the 2019 leadership selection process in the Belgian French-speaking liberal party (MR)
Authors: Legein Thomas , van Haute Emilie
Published: December 2022

This study applies process-tracing methods to understand the 2019 leadership selection process in the Belgian French-speaking liberal party, MR, which is the oldest and second largest party in French-speaking Belgium. We triangulate a variety of sources to assemble a rich qualitative material that is used to contrast the formal rules and outcome of the race to the actual process. We show that the gatekeepers were not the ones ascribed in the statutes, that formal rules were bent to fit the profile of the race, and that the very nature of the race was much closer to a coronation than the results may suggest. We also uncover mechanisms through which party actors, especially the incumbent leader as steering agent, informally influence the process to the desired outcome, with the race being played prior to the validation of the candidacies. This analysis puts focuses onwhenandhowkey actors use their informal influence to weigh the process and influence the outcome of leadership races.
Identifying and dealing with student errors in the mathematics classroom: Cognitive and motivational requirements
Authors: Hoth Jessica , Larrain Macarena , Kaiser Gabriele
Published: December 2022

Introduction

Mathematics classrooms are typically characterized by considerable heterogeneity with respect to students’ knowledge and skills. Mathematics teachers need to be highly attentive to students’ thinking, learning difficulties, and any misconceptions that they may develop. Identification of potential errors and appropriate ways to approach them is crucial for attaining positive learning outcomes. This paper explores which knowledge and affective-motivational skills teachers most require to effectively identify and approach students’ errors.

Methods

To address this research question within the German follow-up study of the Teacher Education and Development Study in Mathematics (TEDS-M), 131 primary school mathematics teachers’ ability to identify students’ errors was assessed based on (a) a digitalized speed test showing different students’ solutions in a written notation and (b) three video vignettes that showed different scenes from mathematics classes. These scenes dealt, among other things, with children who struggled with the lesson’s mathematical content. Teachers were asked to analyze students’ thinking and to determine how best to react. In addition, teachers’ mathematics pedagogical content knowledge, mathematical content knowledge, and beliefs were assessed in separate tests and served as predictors for teachers’ abilities to identify, analyze, and deal with students’ errors.

Results

The results indicate that all components are interrelated. However, path analysis reveals that teachers’ ability to deal with students’ errors is mainly predicted by their constructivist beliefs while their ability to quickly identify typical students’ errors is largely dependent on their mathematics content knowledge.

Discussion

The results show the central filtering function of beliefs. Teachers who believe that students must shape and create their own learning processes are more successful in perceiving and analyzing student errors in classroom situations. They may understand errors as learning opportunities and - thus - pay specific attention to these occurrences.
Exploring the underlying mechanism between fear of losing power and knowledge hiding
Authors: Iqbal Omer , Ali Zeeshan , Azam Akbar
Published: December 2022

Introduction

Drawing on the assumptions of approach/inhibition theory of power and conservation of resource of theory, this study aims to empirically explore the relationship between fear of losing power and knowledge hiding. To explicate the relationship, this study examines the mediating role of self-serving behavior and moderating role of personal competitiveness.

Methods

To evaluate the relationships, a moderated-mediation model is devised and tested. Data is collected through a web-based questionnaire from 194 individuals employed in both manufacturing and service sector firms of Pakistan. Multiple statistical software packages are used to analyze the data.

Results

After employing several statistical techniques, the findings of the study suggest that self-serving behavior fully mediates the link between fear of losing power and knowledge hiding. Moreover, the result of two-way interaction reveals that personal competitiveness further amplifies the indirect relationship between fear of losing power and knowledge hiding through self-serving behavior.

Discussion

The present study is one of those few types that investigates and uncovers the hidden links between fear of losing power and knowledge hiding. Lastly, theoretical, and practical implications along with future research directions are discussed.
Distinct profiles of functional connectivity density aberrance in Alzheimer's disease and mild cognitive impairment
Authors: Miao Dawei , Zhou Xiaoguang , Wu Xiaoyuan , Chen Chengdong , Tian Le
Published: December 2022

Introduction

Investigating the neuroimaging changes from mild cognitive impairment (MCI) to Alzheimer's disease (AD) is of great significance. However, the details about the distinct functional characteristics of AD and MCI remain unknown.

Methods

In this study, we investigated distinct profiles of functional connectivity density (FCD) differences between AD and MCI compared with the normal population, aiming to depict the progressive brain changes from MCI to AD. As a data-driven method, FCD measures the profiles of FC for the given voxel at different scales. Resting-state functional magnetic resonance imaging (fMRI) images were obtained from patients with AD and MCI and matched healthy controls (HCs). One-way ANCOVA was used to investigate (global, long-range, and local) FCD differences among the three groups followed by post-hoc analysis controlling age, sex, and head motion.

Results

The three groups exhibited significant global FCD differences in the superior frontal gyrus. The post-hoc results further showed that patients with AD had a significant increase in global FCD values than those with MCI and HCs. Patients with MCI exhibited an increased trend compared with HCs. We further identified brain regions contributing to the observed global FCD differences by conducting seed-based FC analysis. We also identified that the observed global FCD differences were the additive effects of altered FC between the superior frontal gyrus and the posterior default model network.

Discussion

These results depicted the global information communication capability impairment in AD and MCI providing a new insight into the progressive brain changes from MCI to AD.
Determinants of quality of life improvements in anxiety and depressive disorders—A longitudinal study of inpatient psychotherapy
Authors: Freidl Marion , Wegerer Melanie , Litvan Zsuzsa , König Daniel , Alexandrowicz Rainer W. , Portela-Millinger Filipe , Gruber Maria
Published: December 2022

Background

Quality of life (QoL) is substantially impaired in patients with anxiety disorders (AD) and depressive disorders (DD) and improvements in symptom burden after psychotherapy are not always paralleled by similar improvements in QoL. So far, little is known about treatment outcome in terms of QoL and predictors of QoL improvements following inpatient psychotherapy with a focus on cognitive behavior therapy (CBT). The current study aimed at investigating the relationship between changes in symptoms and QoL across different life domains. Additionally, predictors of a positive treatment outcome were evaluated.

Methods

122 patients with AD and/or DD undergoing an 8-weeks inpatient CBT program completed self-report measures of psychopathological symptoms and QoL at pre- and post-treatment. Mixed effects models were used to investigate changes, a confirmatory factor analysis was applied to analyze the latent factor structure of the anxiety sensitivity index and binary logistic regression analyses were performed for predictors of QoL improvements.

Results

Patients showed moderate to strong decreases in anxious and depressive symptoms and moderate to strong improvements in general QoL, particularly in the psychological and physical QoL subdomains. Changes in symptom burden correlated most strongly with psychological and physical QoL. In addition, poor QoL before treatment and low levels of specific anxiety sensitivity symptoms (items 1 and 5) significantly predicted improvement in QoL.

Conclusion

Patients with poor QoL who are not as inhibited to openly express their anxious feelings particularly benefit from inpatient psychotherapy (individual and group) to improve their QoL. In contrast, our research suggests that patients who are too anxious to openly express their nervousness should receive additional social skills training, more focused treatment to build sufficient self-confidence to better engage in the treatment program.
Intestinal colonization with ESBL-producing Klebsiella pneumoniae in healthy rural villager: A genomic surveillance study in China, 2015-2017
Authors: Wang Shuang , Xie Hengjie , Chen Yuzhen , Liu Lu , Fang Ming , Sun Dapeng , Xu Liuchen , Bi Zhenqiang , Sun Gaoxiang , Li Yan , Yu Xiaolin , Zhang Huaning , Kou Zengqiang , Zheng Beiwen
Published: December 2022

Background

The worldwide emergence and diffusion of extended-spectrum β-lactamase-K. pneumoniae (ESBL-KP) is of particular concern. Although ESBL-KP can inhabit the human gut asymptomatically, colonization with ESBL-KP is associated with an increased risk of ESBL-KP infection and mortality. In this study, we investigated the prevalence and characteristics of ESBL-KP in fecal samples from healthy persons in 12 villages in Shandong Province, China.

Methods

Screening for ESBL-KP in fecal samples was performed by selective cultivation. The bacterial species were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequence analysis. Minimum inhibitory concentrations (MICs) of 16 antibiotics were determined by the agar dilution method. Plasmid replicons, antimicrobial resistance genes and Sequence types (STs) of the isolates were determined by whole-genome sequencing (WGS). Genetic relatedness of ESBL-KP isolates was determined by the single nucleotide polymorphisms (SNP). The S1 nuclease-pulsed-field gel electrophoresis (S1-PFGE) was used to characterize the plasmids carried by ESBL-KP isolates. Conjugation assays was used to verify the transferability of blaCTX − M.

Results

ESBL-KP prevalence rates increased from 12.0% in 2015 to 27.5% in 2017. The experimental results showed that 97% of isolates had multi-drug resistance. Multiple ESBL resistance genotypes were commonly detected in the isolates. STs among the ESBL-KP isolates were diverse. All 69 blaCTX−M−3-positive isolates were located on plasmids, and these genes could be transferred with plasmids between different strains. Phylogenetic analysis showed the possibility of transmission among some isolates.

Conclusion

This study obtained the drug resistance patterns, the drug resistance phenotype and molecular characteristics of fecal-derived ESBL-KP in rural communities in Shandong Province, China. We report a rapid increase in occurrence of ESBL-KP among fecal samples collected from healthy rural residents of Shandong Province from 2015 to 2017. The carriage rate of multidrug-resistant bacteria in healthy residents is increasing. Thus, a need for further monitoring and possible interventions of ESBL-KP in this region is warranted.
Development, testing and validation of a SARS-CoV-2 multiplex panel for detection of the five major variants of concern on a portable PCR platform
Authors: Stanhope Bryce J. , Peterson Brittany , Knight Brittany , Decadiz Ray Nobles , Pan Roger , Davis Phillip , Fraser Anne , Nuth Manunya , vanWestrienen Jesse , Wendlandt Erik , Goodwin Bruce , Myers Christopher , Stone Jennifer , Sozhamannan Shanmuga
Published: December 2022

Many SARS-CoV-2 variants have emerged during the course of the COVID-19 pandemic. These variants have acquired mutations conferring phenotypes such as increased transmissibility or virulence, or causing diagnostic, therapeutic, or immune escape. Detection of Alpha and the majority of Omicron sublineages by PCR relied on the so-called S gene target failure due to the deletion of six nucleotides coding for amino acids 69–70 in the spike (S) protein. Detection of hallmark mutations in other variants present in samples relied on whole genome sequencing. However, whole genome sequencing as a diagnostic tool is still in its infancy due to geographic inequities in sequencing capabilities, higher cost compared to other molecular assays, longer turnaround time from sample to result, and technical challenges associated with producing complete genome sequences from samples that have low viral load and/or high background. Hence, there is a need for rapid genotyping assays. In order to rapidly generate information on the presence of a variant in a given sample, we have created a panel of four triplex RT-qPCR assays targeting 12 mutations to detect and differentiate all five variants of concern: Alpha, Beta, Gamma, Delta, and Omicron. We also developed an expanded pentaplex assay that can reliably distinguish among the major sublineages (BA.1–BA.5) of Omicron. In silico, analytical and clinical testing of the variant panel indicate that the assays exhibit high sensitivity and specificity. This panel can help fulfill the need for rapid identification of variants in samples, leading to quick decision making with respect to public health measures, as well as treatment options for individuals. Compared to sequencing, these genotyping PCR assays allow much faster turn-around time from sample to results—just a couple hours instead of days or weeks.
Quality of therapy and mental health among occupational therapists during the COVID-19 pandemic
Authors: Ito Ayahito , Sawamura Daisuke , Kajimura Shogo , Miyaguchi Hideki , Nakamura Haruki , Ishioka Toshiyuki
Published: December 2022

Introduction

The coronavirus disease of 2019 (COVID-19) has had a severe psychological impact on occupational therapists. Clarifying the mental health status of occupational therapists and its relationship with therapy quality is essential for maintaining the quality of care and patients' quality of life. Therefore, the present study aimed to investigate whether and how mental health problems are related to the quality of occupational therapy.

Methods

A nationwide cross-sectional online survey was conducted during Japan's second national state of emergency (January 2021). A total of 4,418 registered occupational therapists who were members of the Japanese Association of Occupational Therapists participated in this study. After screening for the exclusion criteria, data from 1,966 participants were analyzed.

Results

Path analysis showed that insufficient information provision by the workplace and increased workload were associated with depression, anxiety, and insomnia. Specifically, depression was associated with decreased therapy quality. Furthermore, one's therapy quality showed a strong positive correlation with colleagues' therapy quality.

Discussion

These results demonstrated a direct link between therapists' mental health conditions and therapy quality and suggested that decreased therapy quality might occur at the institutional rather than individual level. A reassessment of the support system and prompt detection and support for professionals with psychological symptoms may be the key to enhancing therapy quality and patients' quality of life. The present results contribute to the understanding of these relationships, considering the current pandemic context for occupational therapists.
Income-related health inequality among rural residents in western China
Authors: Li Chaofan , Tang Chengxiang
Published: December 2022

Objective

Health equality has drawn much public attention in both developed and developing countries. China, the largest developing country, has implemented a new round of health system reform to improve health equality since 2009. This study aims to examine the magnitude and sources of income-related health inequality in western rural regions of China.

Methods

Data were obtained from the Survey of Rural Economic and Social Development in Western China conducted in 2014, in which 14,555 individuals from 5,299 households in 12 provinces were included. Health outcome variables of interest were self-rated health status, prevalence of chronic disease and four-week illness. Concentration index was calculated to assess magnitude of income-related health inequality, and nonlinear decomposition analysis was performed to identify the sources of health inequality.

Results

The Concentration indexes for poor self-rated health status, prevalence of chronic disease and four-week illness were −0.0898 (P<0.001),−0.0860 (P<0.001) and −0.1284 (P<0.001), respectively. Income and education were two main sources of health inequality, accounting for about 25−50% and 15% contribution to the inequality. Ethnicity made <10% contribution to income-related health inequality, and enrollment in New Rural Cooperative Medical Scheme contributed to <1%.

Conclusion

This study found slight income-related health inequality among rural residents in western China, implying that although China has made substantial progress in economic development and poverty alleviation, health inequality in western rural region should still be concerned by the government. To achieve health equality further, the Chinese government should not only strengthen its reimbursement mechanism of the current health insurance scheme to improve affordability of primary healthcare for residents in western rural regions, but also implement health poverty alleviation policies targeting socioeconomically vulnerable population and ethnic minorities in future.
Association between mobile phone addiction and social support among mainland Chinese teenagers: A meta-analysis
Authors: Wan Xiao , Huang Haitao , Jia Ruiying , Liang Dandan , Lu Guangli , Chen Chaoran
Published: December 2022

Background

Mobile phone addiction brings many adverse effects to teenagers, such as physical health problems, emotional problems, and academic failure, and studies have found that social support is an important influencing factor. Therefore, considering institutional, cultural and economic differences, we aimed to investigate the association between mobile phone addiction and social support among mainland Chinese teenagers, and explored the moderators affecting the relation.

Methods

Based on the PRISMA method, a meta-analysis was applied to quantitatively synthesize relevant findings to obtain reliable estimates of effect sizes and conduct moderator analyses.

Results

In total, 92 studies involving 59,716 participants and 92 effect sizes were identified by a systematic literature search. A significant low degree of negative correlation was found between mobile phone addiction and social support (r = −0.174, 95%CI = −0.213 to −0.134, p < 0.001, I2 = 96.1%). Moreover, the present meta-analysis observed significant moderating effects of participants' gender, and region on the association between social support and mobile phone addiction.

Conclusion

This study suggests that the mobile phone addiction level of teenagers could be reduced by increasing social support, and actions to improve their social support levels should be proposed based on their gender and regional differences.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021276672.
Acceptance of the COVID-19 vaccine booster dose and associated factors among the elderly in China based on the health belief model (HBM): A national cross-sectional study
Authors: Qin Chenyuan , Yan Wenxin , Du Min , Liu Qiao , Tao Liyuan , Liu Min , Liu Jue
Published: December 2022

Background

The reluctance of individuals to obtain solid vaccine-induced immunity represents a fundamental challenge to containing the spread of SARS-CoV-2, including its highly mutated variants. We aimed to assess vaccination acceptance and associated factors for the COVID-19 vaccine booster dose among elderly people (≥60 years old) in China, providing a theoretical and practical reference for universal vaccination policy.

Methods

A national anonymous survey was conducted in mainland China from May 25 to June 8, 2022, using a stratified random sampling method. Individuals 60 years of age and above were the target population. A chi-squared test and Cochran-Armitage test for trend were used to compare and examine vaccine acceptance rates by characteristics. Via a backward stepwise method, multivariable logistic regression models were established to assess factors associated with booster dose acceptance. Two-sided P < 0.05 was considered statistically significant.

Results

Of 3,321 eligible participants, 82.8% (95% CI: 81.5–84.1%) were willing to receive COVID-19 vaccine booster shots. Concerns about contraindications (38.3%), vaccine safety (32.0%), and limited movement (28.0%) were the main reasons for vaccine hesitancy. Nearly one-third still believed that the booster dose was unnecessary after receiving the initial vaccination. Older adults with a low level of perceived barriers (aOR = 1.86, 95% CI, 1.03–3.38), a high level of perceived benefit (aOR = 2.31, 95% CI, 1.38–3.87), and higher cues to action (moderate, aOR = 2.22, 95% CI, 1.39–3.56; high, aOR = 5.46, 95% CI: 3.44–8.67) were more likely to accept the booster dose. Other major factors affecting the booster dose acceptance rate were occupation, time spent on social media, vaccination history, and a high knowledge score for COVID-19 and vaccines. In addition, for those over 70 years of age, rising awareness of susceptibility could be a better gateway for improving their willingness to get vaccinated.

Conclusions

A total of 82.8% of recruited older adults were willing to receive the booster dose. Acceptance behaviors were closely related to occupation, time spent on social media, vaccination history, knowledge factors, perception of barriers, and benefit, as well as action cues. Targeted public health measures are a priority for improving the vaccination coverage of valid immunity among the elderly population, not only to prevent infection and poor prognosis caused by emerging variants but also to reduce the huge disease and economic burden caused by the long-term sequelae after SARS-CoV-2 infection.
Comparison of virtual reality platforms to enhance medical education for procedures
Authors: Shah Nishant K. , Taunk Neil K. , Maxwell Russell , Wang Xingmei , Hubley Emily , Anamalayil Shibu , Trotter Jacob W. , Li Taoran
Published: December 2022

Background: Historically, medical education relied on apprentice-based experiences requiring direct observation in patient cases. Simulation-based education has been shown to improve resident confidence but can be time intensive and difficult to coordinate. The COVID-19 pandemic demonstrated the need to develop distributed educational tools. Virtual reality (VR) platform has been shown to improve resident confidence and proficiencies. This pilot study compared educational and cost effectiveness of low-cost cardboard viewer VR (CVVR) and commercially available integrated headset VR (IHVR).

Methods and Materials: We created a 2D, 360-degree VR video of an intracavitary brachytherapy case for treatment of cervical cancer. Radiation oncology residents from a single ACGME-accredited training program were recruited and randomized to IHVR or CVVR. Both groups were given unlimited access to their randomized technology. Each resident performed a timed intracavitary procedure on a simulator while five implant quality metrics were recorded. A pre- and post-simulation questionnaire assessed self-confidence, procedural knowledge, and perceived usefulness of VR technology.

Results: There were 13 residents, including four post-graduate year (PGY)-2, three PGY-3, two PGY-4, and four PGY-5, in the study. Both VR technologies improved self-perceived overall confidence. Average time required for implant (mean: CVVR - 200 s vs IHVR - 235 s, p = 0.38) and median objective proficiencies of implant quality (5/5 in both group, p = 0.56) were similar. There was no difference between CVVR and IHVR as useful, enjoyable and engaging educational tool. Both groups would recommend the technology to another trainee. IHVR-based program would cost ∼33x more than CVVR-based program based on an assessment of US-based programs.

Conclusion: CVVR is a cost-effective alternative to a IHVR as a virtual video-based education tool.
Quality control and purification of ready-to-use conjugated gold nanoparticles to ensure effectiveness in biosensing
Authors: Wang Junjie , Giordani Stefano , Marassi Valentina , Roda Barbara , Reschiglian Pierluigi , Zattoni Andrea
Published: December 2022

Introduction: Gold nanoparticles (AuNPs) and their conjugates are used for many applications in the field of sensors. Literature lacks procedures able to separate, purify and characterize these species in native conditions without altering them while assuring a high throughput. This technological gap can be reduced by exploiting Asymmetrical Flow Field Flow Fractionation multidetection platforms (AF4 multidetection).

Method: This work describes a complete set of strategies based on the AF4 system, from nanoparticle synthesis to separative method optimization to conjugates screening and characterization, achieving quantitative control and purification of ready-to-use conjugated Gold nanoparticles and ensuring effectiveness in biosensing.

Results and Discussion: AF4-multidetection was used to study AuNPs with different types of surface coating [Poly ethylene glycol, (PEG) and Citrate], their binding behaviour with protein (Bovine serum albumin, BSA) and their stability after conjugation to BSA. A robust but flexible method was developed, able to be applied to different AuNPs and conjugating molecules. The morphology and conjugation mechanism of AuNPs-BSA conjugates were evaluated by combining online Multiangle light scattering (MALS) and offline Dynamic Light Scattering (DLS) measures, which provided an important feature for the quality control required to optimize bio-probe synthesis and subsequent bioassays.
Kinematics of the diffuse intragroup and intracluster light in groups and clusters of galaxies in the local universe within 100 Mpc distance
Authors: Arnaboldi Magda , Gerhard Ortwin
Published: December 2022

Nearly all intragroup (IGL) and intracluster light (ICL) comes from stars that are not bound to any single galaxy but were formed in galaxies and later unbound from them. In this review we focus on the physical properties–phase space properties, metallicity and age distribution–of the ICL and IGL components of the groups and clusters in the local universe, within 100 Mpc distance. Kinematic–information on these very low surface brightness structures mostly comes from discrete tracers such as planetary nebulae and globular clusters, showing highly unrelaxed velocity distributions. Cosmological hydrodynamical simulations provide key predictions for the dynamical state of IGL and ICL and find that most IC stars are dissolved from galaxies that subsequently merge with the central galaxy. The increase of the measured velocity dispersion with radius in the outer halos of bright galaxies is a physical feature that makes it possible to identify IGL and ICL components. In the local groups and clusters, IGL and ICL are located in the dense regions of these structures. Their light fractions relative to the total luminosity of the satellite galaxies in a given group or cluster are between a few to ten percent, significantly lower than the average values in more evolved, more distant clusters. IGL and ICL in the Leo I and M49 groups, and the Virgo cluster core around M87, has been found to arise from mostly old (≥10 Gyr) metal-poor ([Fe/H] <-1.0) stars of low-mass progenitor galaxies. New imaging facilities such as LSST, Euclid, and the “big eyes’’ on the sky–ELT and JWST with their advanced instrumentation–promise to greatly increase our knowledge of the progenitors of the IGL and ICL stars, their ages, metal content, masses and evolution, there by increasing our understanding of this enigmatic component.
Portuguese Physical Literacy Assessment - Observation (PPLA-O) for adolescents (15–18 years) from grades 10–12: Development and initial validation through item response theory
Authors: Mota João , Martins João , Onofre Marcos
Published: December 2022

Introduction

Aims of these studies were to develop the Portuguese Physical Literacy Assessment Observation instrument (PPLA-O) to assess the physical and part of the cognitive domain of Physical Literacy (PL) through data collected routinely by Physical Education (PE) teachers; and to assess the construct validity (dimensionality, measurement invariance, and convergent and discriminant validity) and score reliability of one of its modules [Movement Competence, Rules, and Tactics (MCRT)].

Methods

Content analysis of the Portuguese PE syllabus and literature review were used for PPLA-O domain identification. Multidimensional Item Response Theory (MIRT) models were used to assess construct validity and reliability, along with bivariate correlations in a sample of 515 Portuguese grade 10–12 students (Mage = 16, SD = 1).

Results

PPLA-O development resulted in an instrument with two modules: MCRT (22 physical activities) and Health-Related Fitness (HRF; 5 protocols); both assessed with teacher-reported data entered in a spreadsheet. A two correlated dimensions Graded Response Model (Manipulative-based Activities [MA], and Stability-based Activities [SA]) showed best fit to the MCRT data, suggesting measurement invariance across sexes, and adequate to good score reliabilities (MA = .89, and SA = .73). There was a moderate to high correlation (r = .68) between dimensions, and boys had higher scores in both dimensions. Correlations among MCRT scores and HRF variables were similar in magnitude to previous reports in meta-analysis and systematic reviews.

Conclusions

PPLA-O is composed of two modules that integrate observational data collected by PE teachers into a common frame of criterion-referenced PL assessment. The HRF module uses data collected through widely validated FITescola® assessment protocols. The MCRT makes use of teacher-reported data collected in a wide range of activities and movement pursuits to measure movement competence and inherent cognitive skills (Tactics and Rules). We also gathered initial evidence supporting construct validity and score reliability of the MCRT module. This highly feasible instrument can provide Portuguese grade 10–12 (15–18 years) PE students with feedback on their PL journey, along with the other instrument of PPLA (PPLA-Questionnaire). Further studies should assess inter and intra-rater reliability and criterion-related validity of its two modules.
Transfer of sustainability training in land and conservation agriculture project: A behavioral study in Iran
Authors: Ataei Pouria , Sadighi Hassan , Abbasi Enayat , Chizari Mohammad
Published: December 2022

Introduction

The transfer of sustainability training means applying the skills, attitudes, and knowledge acquired from training to the workplace to help sustainability and environmental protection. This study aimed to conduct a behavioral analysis of sustainability training transfer among farmers that had attended extension-training courses on the Land and Conservation Agriculture Project (LCAP) in Iran.

Methods

This research is quantitative and applied research that was carried out via a standardized survey. The research population was composed of all farmers who attended LCAP extension training courses in the three provinces of Golestan, Fars, and Khouzestanin, Iran (N=1204). 291 farmers were selected through stratified random sampling. A panel of experts established the content and face validity of the questionnaire. A pilot study was conducted to check the reliability by calculating Cronbach's alpha.

Results and discussion

Findings indicated that most farmers had sustainability training transfer at a high level. The analysis with a structural equation model revealed that perceived content validity, transfer design, opportunity to use, supervisor support, trainer characteristics, peer support, motivation to transfer, transfer effort -performance expectations, performance coaching, and personal capacity for transfer influenced farmers' intention to transfer sustainability training significantly and positively. Furthermore, farmers' intentions had a significant effect on their transfer of sustainability training. It can be concluded that a sustainability training transfer system is applicable to a training context related to sustainable agriculture and can provide a useful instrument for agricultural education organizations when investing in farmers' training.
Survival in canine tetanus – retrospective analysis of 42 cases (2006–2020)
Authors: Zitzl Johannes , Dyckers Jens , Güssow Arne , Lehmann Hendrik , Hazuchova Katarina
Published: December 2022

Objective

To define factors associated with survival in dogs with tetanus and to evaluate the prognostic significance of an established severity classification scheme.

Methods

Medical records of dogs with the clinical diagnosis of tetanus were retrospectively reviewed with regard to signalment, clinical signs, clinicopathological findings on admission, wound characteristics, complications, therapeutic measures, and survival to discharge. Based on the extracted data, dogs were graded according to a previously published 4-class severity scheme. Non-parametric tests were applied for comparisons between survival categories.

Results

Forty-two dogs fulfilled inclusion criteria, of which 32 survived. Of 10 non-survivors, 4 died and 6 were euthanised. Non-survivors were more often younger than 2 years of age (6/10 vs. 7/32 dogs,p= 0.023), had shorter duration of specific signs of tetanus (time from onset of typical signs to presentation) (2 vs. 4 days,p= 0.016), were prescribed less often antibiotics prior to presentation (p= 0.006), had higher tetanus severity grade (10/12 dogs in Class III or IV died,p< 0.001), more often received acepromazine (p= 0.009) and atropine (p= 0.012), and more often had hyperthermia (p= 0.005) and respiratory complications (pneumonia, laryngeal spasm;p= 0.008). Wound characteristics, the use of tube feeding, metronidazole, methocarbamol, magnesium and antitoxin were not significantly different between non-survivors and survivors.

Clinical significance and conclusion

Young dogs with a rapid course of severe generalized tetanus have a guarded prognosis. The previously described severity classification scheme proved valuable in predicting survival. Prospective multi-center studies are needed to clarify the prognostic significance of age, sedative usage and modified versions of an established classification scheme, including the presence of respiratory complications.
SARS-CoV-2 and animals, a long story that doesn't have to end now: What we need to learn from the emergence of the Omicron variant
Authors: Reggiani Alessandro , Rugna Gianluca , Bonilauri Paolo
Published: December 2022

OIE, the world organization for animal health, recently released an update on the state of the art of knowledge regarding SARS-CoV-2 in animals. For farmed animals, ferrets and minks were found to be highly susceptible to the virus and develop symptomatic disease both in natural conditions and in experimental infections. Lagomorphs of the species Oryctolagus cuniculus are indicated as highly susceptible to the virus under experimental conditions, but show no symptoms of the disease and do not transmit the virus between conspecifics, unlike raccoon dogs (Nyctereutes procyonoides), which in addition to being highly susceptible to the virus under experimental conditions, can also transmit the virus between conspecifics. Among felines, the circulation of the virus has reached a level of cases such as sometimes suggests the experimental use of vaccines for human use or treatments with monoclonal antibodies. But even among wild animals, several species (White-tailed deer, Egyptian rousettes, and minks) have now been described as potential natural reservoirs of the virus. This proven circulation of SARS-CoV-2 among animals has not been accompanied by the development of an adequate surveillance system that allows following the evolution of the virus among its natural hosts. This will be all the more relevant as the surveillance system in humans inevitably drops and we move to surveillance by sentinels similar to the human flu virus. The lesson that we can draw from the emergence of Omicron and, more than likely, its animal origin must not be lost, and in this mini-review, we explain why.
Use of a new antimicrobial consumption monitoring system (Vet-AMNet): Application to Dutch dairy sector over a 9-year period
Authors: Moura Pedro , Sanders Pim , Heederik Dick , Van Geijlswijk Ingeborg Marianne , Niza-Ribeiro João
Published: December 2022

Introduction

The urgency of preventing the increase of antimicrobial resistance has been emphasized by international authorities such as the World Health Organization, European Medicines Agency, and World Organization for Animal Health. Monitoring systems capable of reporting antimicrobial consumption data are regarded as a crucial pillar of this fight. The Vet-AMNet system was developed to collect and analyze national antimicrobial consumption data in Portuguese dairy farms to support the veterinary authority in stewardship actions and to assist both veterinarians and farmers in daily decisions related to antimicrobials.

Methods

To evaluate the robustness of the system and other identified critical success factors, it was used to analyze antimicrobial consumption data available from the Dutch dairy cow sector over the period from 2012 to 2020. The data previously used for publications by the Netherlands Veterinary Medicines Institute (SDa) were imported and pre-processed by the Vet-AMNet system according to the SDa's standard operating procedure and the Dutch metrics to measure antimicrobial consumption were calculated.

Results

By comparing the outputs with the figures generated by the system established in the Netherlands, the Portuguese system was validated. Antimicrobial consumption data from the Dutch dairy sector during the 9-year period will be presented in unpublished graphs and tables, where each molecule's pharmaceutical formulation, pharmacotherapeutic group and line of choice will be related and discussed, illustrating the evolution of sectorial antimicrobial consumption against a background of a strong national antimicrobial policy initiated by public-private cooperation and supported by legislation.
Prevalence of Antimicrobial Resistance and Infectious Diseases in a Hospitalised Migrant Population in Paris, France, a Retrospective Study
Authors: Stabler Sarah , Paccoud Olivier , Duchesne Léa , Piot Marie-Aude , Valin Nadia , Decré Dominique , Girard Pierre-Marie , Lalande Valérie , Lacombe Karine , Surgers Laure
Published: December 2022

Objectives: The aim of this study was to estimate the prevalence of anti-microbial resistance (AMR) carriage and its risk factors in hospitalized migrants. Additionally, the prevalence of infectious diseases was evaluated, as well as symptoms of psychological trauma.

Methods: We conducted a retrospective monocentric cross-sectional study including all migrant patients recently arrived and hospitalised over a one-year period.

Results: Among 101 patients, seventy-nine percent originated from Sub-Saharan Africa. The overall AMR carriage rate was 20.7% [95% CI: 12.4; 28.9%]. We isolated 5/92 methicillin-resistant Staphylococcus aureus strains (5.4%) and 15/92 extended-spectrum beta-lactamase-producing Enterobacteriaceae (16.4%). AMR carriage was associated with older age, region of origin and length of migration. Rates of HIV, HBV, and HCV infection were 39.6%, 32.7%, and 5%, reflecting sampling bias linked to reasons for hospitalization. Eleven percent had serological evidence of treponemasis and 7.8% had Chlamydia trachomatis infection. Symptoms of depression or post-traumatic stress disorder were observed for more than half the patients.

Conclusion: It appears essential to offer a systematic and comprehensive post-arrival screening of AMR carriage, infectious diseases and psychological trauma to subjects who experienced migration.
Progress of Porous/Lattice Structures Applied in Thermal Management Technology of Aerospace Applications
Authors: Liu Jian , Xu Mengyao , Zhang Rongdi , Zhang Xirui , Xi Wenxiong
Published: December 2022

With lightweight, multifunctional, and designable characteristics, porous/lattice structures have started to be used in aerospace applications. Porous/lattice structures applied in the thermal management technology of aerospace vehicles have attracted much attention. In the past few years, many related numerical and experimental investigations on flow, heat transfer, modelling methodology, and manufacturing technology of porous/lattice structures applied in thermal management systems have been widely conducted. This paper lists the investigations and applications of porous/lattice structures applied in thermal management technology from two aspects, i.e., heat transfer enhancement by porous/lattice structures and transpiration cooling. In addition, future developments and challenges based on the previous investigations are analyzed and summarized. With the higher requirements of thermal protection for aerospace applications in the future, thermal management technology based on porous/lattice structures shows good prospects.
Anticancer Effects of High Glucosinolate Synthesis Lines of Brassica rapa on Colorectal Cancer Cells
Authors: Kim Jung Sun , Han Sanghee , Kim Hail , Won So Youn , Park Hyun Woo , Choi Hyunjin , Choi Minji , Lee Min Young , Ha In Jin , Lee Seok-Geun
Published: December 2022

Chemoprevention is a method of health control in modern industrialized societies. Traditional breeding (hybridization) has been widely used to produce new (sub)species with beneficial phenotypes. Previously, we produced a number of doubled haploid (DH) lines of Brassica rapa with a high glucosinolate (GSL) content. In this study, we evaluated the anticancer activities of extracts from three selected high-GSL (HGSL)-containing DH lines (DHLs) of Brassica rapa in human colorectal cancer (CRC) cells. The three HGSL DHL extracts showed anti-proliferative activities in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay and pro-apoptotic activities in the cell cycle or annexin V analysis with the induction of pro-apoptotic protein expression in CRC cells. Mechanistically, HGSL DHL extracts inhibited the NF-κB and ERK pathways, leading to a reduction in the nuclear localization of NF-κB p65. In addition, reactive oxygen species were induced by HGSL DHL extract treatment in CRC cells. In conclusion, our data suggest that the newly developed HGSL DHLs possess enhanced anticancer activities and are potentially helpful as a daily vegetable supplement with chemopreventive activities.
Heavy Ion Beam Probing Diagnostics on the TUMAN-3M Tokamak for Study Plasma Potential and Electric Fields in New Operational Regimes
Authors: Askinazi Leonid , Abdullina Gulnara , Belokurov Alexander , Kornev Vladimir , Lebedev Sergei , Razumenko Dmitri , Shergin Dmitri , Smirnov Alexander , Tukachinsky Alexander , Zhubr Nikolai
Published: December 2022

Heavy Ion Beam Probing (HIBP) diagnostic is a powerful tool for electric field studies in the hot dense plasma of modern-day toroidal magnetic confinement devices. On the TUMAN-3M tokamak, the HIBP have been used in regimes with improved plasma confinement to clear up the role of the radial electric field in the transition to good confinement regimes. Recently, a modernization of the TUMAN-3M HIBP diagnostics was performed, aiming to reconfigure it for a work with a reversed plasma current direction and improvement of the overall stability of the diagnostic. The results of the first measurements of the plasma potential in the co-NBI scenario are reported and discussed.
Nonequilibrium Steady State in a Large Magneto-Optical Trap
Authors: Gaudesius Marius , Zhang Yong-Chang , Pohl Thomas , Labeyrie Guillaume , Kaiser Robin
Published: December 2022

Considering light-mediated long-range interactions between cold atoms in a magneto-optical trap (MOT), we present numerical evidence of a nonequilibrium steady state (NESS) for sufficiently large number of atoms (>108). This state manifests itself as the appearance of an anisotropic distribution of velocity when a MOT approaches the threshold beyond which self-oscillating instabilities occur. Our three-dimensional (3D) spatiotemporal model with nonlocal spatial dependencies stemming from the interatomic interactions has recently been compared successfully to predict different instability thresholds and regimes in experiments with rubidium atoms. The behavior of the NESS is studied as a function of the main MOT parameters, including its spatiotemporal characteristics.