bluuebunny/crossref_metadata_2025 huggingface dataset:
The safety and immunogenicity of a two-dose schedule of CoronaVac, and the immune persistence of vaccination for six months, in people living with HIV: A multicenter prospective cohort study
Authors: Wang Yuxiao , Qiao Ying , Huo Yuqi , Wang Li , Liang Shijie , Yu Maohe , Lan Xinquan , Song Moxin , Zhang Xiangjun , Yan Ying , Xu Junjie
Published: March 2023

Background

People living with HIV (PLWH) are more vulnerable to SARS-CoV-2. However, evidence on the immunogenicity of coronavirus disease 2019 (COVID-19) vaccines in this population is insufficient. The objective of this study is to assess the immunogenicity and safety of the two-dose schedule of Sinovac CoronaVac for 6 months postvaccination in PLWH.

Methods

We conducted a multicenter prospective cohort study among PLWH and HIV-negative adults in China. Participants who received two doses of CoronaVac prior to the recruitment were allocated into two groups and followed up for 6 months. The neutralizing antibodies (nAbs), immunoglobulin G against the receptor-binding domain of the spike protein (S-IgG), and gamma-interferon (IFN-γ) were measured to assess the associations among CoronaVac immunogenicity and related factors. Adverse reactions were collected to evaluate the safety profile of vaccination.

Results

A total of 203 PLWH and 100 HIV-negative individuals were enrolled. A small portion of participants reported mild or moderate adverse reactions without serious adverse events. Median nAbs level in PLWH (31.96 IU/mL, IQR: 12.34-76.40) was lower than that in the control group (46.52 IU/mL, IQR: 29.08-77.30) at the 2-4 weeks postvaccination (P=0.002), and the same trend was presented for median S-IgG titer (37.09 vs. 60.02 IU/ml) (both P <0.05). The nAbs seroconversion rate in the PLWH group was also lower than in the control group (75.86% vs. 89.00%). After then, the immune responses reduced over time in term of only 23.04% of PLWH and 36.00% of HIV-negative individuals had a positive seroconversion for nAbs at 6-month. The multivariable generalized estimating equation analysis showed that PLWH with CD4+T count≥350 cells/µL presented higher immune response than PLWH with CD4+T count <350 cells/µL in terms of antibody seroconversion and titers. The immunogenicity did not differ in participants with low or high HIV viral load. The S-antigen specific IFN-γ immunity was generally stable and had a slow attenuation in both two groups for 6 months postvaccination.

Conclusion

The Sinovac CoronaVac was generally safe and immunogenic in PLWH, but the immunity response was inferior and the antibodies vanished faster compared to HIV-negative individuals. This study suggested a shorter than 6-month interval of prime-boost vaccination for PLWH to ensure a better protection.
The reliability of FEbrile Neutropenia after ChEmotherapy (FENCE) scores in predicting granulocyte colony-stimulating factor breakthrough febrile neutropenia among patients with lymphoma undergoing first-cycle chemotherapy: A prospective observational study
Authors: Thungthong Pravinwan , Chamnanchanunt Supat , Suwanban Tawatchai , Nakhahes Chajchawan , Iam-arunthai Kunapa , Akrawikrai Tananchai , Bunworasate Udomsak , Rojnuckarin Ponlapat
Published: March 2023

Background

A tool for estimating risk of febrile neutropenia (FN) after chemotherapy, namely the FEbrile Neutropenia after ChEmotherapy (FENCE) score, has been developed but has not been widely validated. This study aimed to validate the FENCE score as a tool for predicting granulocyte colony-stimulating factor (G-CSF) breakthrough FN among patients with lymphoma who underwent chemotherapy.

Methods

This was a prospective observational study of treatment-naive adult patients with lymphoma who underwent their first cycle of chemotherapy between 2020 and 2021. The patients were followed up until the next cycle of chemotherapy to identify any infection events.

Results

Among the 135 patients with lymphoma, 62 (50%) were men. In a comparison of the value of each FENCE parameter for predicting G-CSF breakthrough infection, the parameter of advanced-stage disease showed high sensitivity of 92.8%, and receipt of platinum chemotherapy showed high specificity of 95.33%. With a FENCE score of 12 as a cutoff for low risk, analysis across all patients with lymphoma resulted in a high AUROCC of 0.63 (95% CI = 0.5–0.74%; p = 0.059), and analysis across only patients with diffuse large B-cell lymphoma (DLBCL) resulted in an AUROCC of 0.65 (95% CI = 0.51–0.79%; p = 0.046). With a cutoff point of 12, FENCE score can predict breakthrough infection events at 30.0% (95% CI = 17.8–47.4%).

Conclusion

This study divided patients with lymphoma into risk groups according to FENCE score, showing that this instrument has discriminatory ability in predicting FN events, these being more likely to occur in patients in the intermediate- and high-risk groups. Multicenter studies are needed to validate this clinical risk score.
Analysis of gene expression and use of connectivity mapping to identify drugs for treatment of human glomerulopathies
Authors: Chung Chen-Fang , Papillon Joan , Navarro-Betancourt José R. , Guillemette Julie , Bhope Ameya , Emad Amin , Cybulsky Andrey V.
Published: March 2023

Background

Human glomerulonephritis (GN)—membranous nephropathy (MN), focal segmental glomerulosclerosis (FSGS) and IgA nephropathy (IgAN), as well as diabetic nephropathy (DN) are leading causes of chronic kidney disease. In these glomerulopathies, distinct stimuli disrupt metabolic pathways in glomerular cells. Other pathways, including the endoplasmic reticulum (ER) unfolded protein response (UPR) and autophagy, are activated in parallel to attenuate cell injury or promote repair.

Methods

We used publicly available datasets to examine gene transcriptional pathways in glomeruli of human GN and DN and to identify drugs.

Results

We demonstrate that there are many common genes upregulated in MN, FSGS, IgAN, and DN. Furthermore, these glomerulopathies were associated with increased expression of ER/UPR and autophagy genes, a significant number of which were shared. Several candidate drugs for treatment of glomerulopathies were identified by relating gene expression signatures of distinct drugs in cell culture with the ER/UPR and autophagy genes upregulated in the glomerulopathies (“connectivity mapping”). Using a glomerular cell culture assay that correlates with glomerular damage in vivo, we showed that one candidate drug – neratinib (an epidermal growth factor receptor inhibitor) is cytoprotective.

Conclusion

The UPR and autophagy are activated in multiple types of glomerular injury. Connectivity mapping identified candidate drugs that shared common signatures with ER/UPR and autophagy genes upregulated in glomerulopathies, and one of these drugs attenuated injury of glomerular cells. The present study opens the possibility for modulating the UPR or autophagy pharmacologically as therapy for GN.
Environmental factors and host genotype control foliar epiphytic microbial community of wild soybeans across China
Authors: Zhou Rui , Duan Gui-Lan , García-Palacios Pablo , Yang Guang , Cui Hui-Ling , Yan Ming , Yin Yue , Yi Xing-Yun , Li Lv , Delgado-Baquerizo Manuel , Zhu Yong-Guan
Published: March 2023

Introduction

The microbiome inhabiting plant leaves is critical for plant health and productivity. Wild soybean (Glycine soja), which originated in China, is the progenitor of cultivated soybean (Glycine max). So far, the community structure and assembly mechanism of phyllosphere microbial community on G. soja were poorly understood.

Methods

Here, we combined a national-scale survey with high-throughput sequencing and microsatellite data to evaluate the contribution of host genotype vs. climate in explaining the foliar microbiome of G. soja, and the core foliar microbiota of G. soja were identified.

Results

Our findings revealed that both the host genotype and environmental factors (i.e., geographic location and climatic conditions) were important factors regulating foliar community assembly of G. soja. Host genotypes explained 0.4% and 3.6% variations of the foliar bacterial and fungal community composition, respectively, while environmental factors explained 25.8% and 19.9% variations, respectively. We further identified a core microbiome thriving on the foliage of all G. soja populations, including bacterial (dominated by Methylobacterium-Methylorubrum, Pantoea, Quadrisphaera, Pseudomonas, and Sphingomonas) and fungal (dominated by Cladosporium, Alternaria, and Penicillium) taxa.

Conclusion

Our study revealed the significant role of host genetic distance as a driver of the foliar microbiome of the wild progenitor of soya, as well as the effects of climatic changes on foliar microbiomes. These findings would increase our knowledge of assembly mechanisms in the phyllosphere of wild soybeans and suggest the potential to manage the phyllosphere of soya plantations by plant breeding and selecting specific genotypes under climate change.
Regulated control of virus replication by 4-hydroxytamoxifen-induced splicing
Authors: Zhao Zhenghao , Wang Busen , Wu Shipo , Zhang Zhe , Chen Yi , Zhang Jinlong , Wang Yudong , Zhu Danni , Li Yao , Xu Jinghan , Hou Lihua , Chen Wei
Published: March 2023

Designing a modified virus that can be controlled to replicate will facilitate the study of pathogenic mechanisms of virus and virus–host interactions. Here, we report a universal switch element that enables precise control of virus replication after exposure to a small molecule. Inteins mediate a traceless protein splicing–ligation process, and we generate a series of modified vesicular stomatitis virus (VSV) with intein insertion into the nucleocapsid, phosphoprotein, or large RNA-dependent RNA polymerase of VSV. Two recombinant VSV, LC599 and LY1744, were screened for intein insertion in the large RNA-dependent RNA polymerase of VSV, and their replication was regulated in a dose-dependent manner with the small molecule 4-hydroxytamoxifen, which induces intein splicing to restore the VSV replication. Furthermore, in the presence of 4-hydroxytamoxifen, the intein-modified VSV LC599 replicated efficiently in an animal model like a prototype of VSV. Thus, we present a simple and highly adaptable tool for regulating virus replication.
Quality control procedures and metrics for resting-state functional MRI
Authors: Birn Rasmus M.
Published: March 2023

The monitoring and assessment of data quality is an essential step in the acquisition and analysis of functional MRI (fMRI) data. Ideally data quality monitoring is performed while the data are being acquired and the subject is still in the MRI scanner so that any errors can be caught early and addressed. It is also important to perform data quality assessments at multiple points in the processing pipeline. This is particularly true when analyzing datasets with large numbers of subjects, coming from multiple investigators and/or institutions. These quality control procedures should monitor not only the quality of the original and processed data, but also the accuracy and consistency of acquisition parameters. Between-site differences in acquisition parameters can guide the choice of certain processing steps (e.g., resampling from oblique orientations, spatial smoothing). Various quality control metrics can determine what subjects to exclude from the group analyses, and can also guide additional processing steps that may be necessary. This paper describes a combination of qualitative and quantitative assessments to determine the quality of fMRI data. Processing is performed using the AFNI data analysis package. Qualitative assessments include visual inspection of the structural T1-weighted and fMRI echo-planar images, functional connectivity maps, functional connectivity strength, and temporal signal-to-noise maps concatenated from all subjects into a movie format. Quantitative metrics include the acquisition parameters, statistics about the level of subject motion, temporal signal-to-noise ratio, smoothness of the data, and the average functional connectivity strength. These measures are evaluated at different steps in the processing pipeline to catch gross abnormalities in the data, and to determine deviations in acquisition parameters, the alignment to template space, the level of head motion, and other sources of noise. We also evaluate the effect of different quantitative QC cutoffs, specifically the motion censoring threshold, and the impact of bandpass filtering. These qualitative and quantitative metrics can then provide information about what subjects to exclude and what subjects to examine more closely in the analysis of large datasets.
Design of a randomized cross-over study evaluating effects of carbohydrate intake on glycemic control in persons with type 1 diabetes
Authors: Sterner Isaksson Sofia , Ólafsdóttir Arndís F. , Lind Marcus
Published: March 2023

Introduction

Diet is an important factor in managing glycemic control in type 1 diabetes (T1D). Reducing carbohydrate intake may be important for stabilizing blood glucose levels in certain groups of patients with T1D. There are few studies examining the effects of a low carbohydrate diet in patients with T1D. The aim of this study is to investigate the effects of carbohydrate intake on glucose control in adults with T1D.

Materials and methods

Adults with T1D (N = 54) and inadequate glycemic control (HbA1c ≥ 7.5%; 58 mmol/mol) were randomized in a cross-over design to a moderate carbohydrate diet (30 percent of total energy from carbohydrates) versus a traditional diabetes diet (50 percent of total energy from carbohydrates) for 4 weeks with a between wash-out period of 4 weeks. Masked continuous glucose monitoring was used throughout the study to evaluate effects on mean blood glucose levels, time-in-range, hypoglycemia, hyperglycemia, and glycemic variability. Diabetes treatment satisfaction, hypoglycemic confidence, and physical activity were measured using questionnaires during different phases of the trial. HbA1c, blood lipids, blood pressure, and ketone levels were also measured. The primary endpoint is the difference in mean blood glucose level between the diet periods. Study completion is anticipated during winter 2022.

Discussion

The study seeks to increase knowledge about the effects of dietary carbohydrate intake on glycemic control and other health parameters in patients with T1D. If beneficial effects on mean blood glucose level without elevated risk of hypoglycemia or ketoacidosis are shown, a moderate carbohydrate diet may be a treatment option for people with T1D that have unsatisfactory blood glucose levels.

Clinical Trials Registration:www.clinicaltrials.gov, ID: NCT03400618.
Indo-cyanine green administration to identify loss of lymph after axillary lymph node dissection
Authors: Roman Mirela Mariana , Delrue Pauline , Karler Clarence , Del Marmol Véronique , Bourgeois Pierre
Published: March 2023

Background

Near infrared fluorescence imaging with Indocyanine Green (ICG) is now used for the imaging of lymph nodes and lymphatic vessels. In this work, we investigated the impact of its pre-operative and peri-operative administration on our ability to detect axillary lymphatic loss after breast cancer surgery.

Methods

One subcutaneous injection of ICG was administered in the ipsilateral hand of 109 women who were scheduled to have either a mastectomy with total axillary lymph node dissection (CALND) or a lumpectomy with selective lymphadenectomy (SLN) the day before (n = 53) or the same day of surgery (n = 56). The lymph leakages were assessed by means of the application of a compress in the operated armpit and by the presence or absence of fluorescence on it, as well as in the post-operative axillary drains.

Results

The compress was fluorescent in 28% of SLN patients and 71% of CALND patients. The liquids in the axillary drains were also fluorescent in 71% of patients with CALND. No statistical significance was observed between the ICG injection groups. The association between compressive fluorescent and the presence of fluorescence in the axillary drains is significant in the pre-operative subgroup and in the whole group.

Conclusion

Our research demonstrates that lymphatic leaks aid in the development of seromas and calls into question the effectiveness of the ligatures and/or cauterizations used during surgery. A prospective, multicentric, randomized trial should be conducted to verify the efficacy of this approach.
Adjuvant EGFR-TKI therapy in resected EGFR-mutation positive non-small cell lung cancer: A real-world study
Authors: Liu Jun-Feng , Sun Xu-Sheng , Yin Jin-Huan , Xu Xi-E
Published: March 2023

Background

Although several clinical studies have laid the foundation for the adjuvant application of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), some questions remain unresolved. This real-world study aimed to address questions such as the effect of adjuvant chemotherapy prior to adjuvant EGFR-TKI therapy on survival outcomes, and the duration of adjuvant EGFR-TKI therapy, etc.

Methods

Between October 2005 and October 2020, 227 consecutive patients with non-small cell lung cancer (NSCLC) who underwent complete pulmonary resections were included in this retrospective study. Patients received postoperative adjuvant chemotherapy followed by EGFR-TKI or adjuvant EGFR-TKI monotherapy. The disease-free survival (DFS) and overall survival (OS) were evaluated.

Results

Of the total 227 patients, 55 (24.2%) patients underwent 3-4 cycles of chemotherapy prior to receiving adjuvant EGFR-TKI therapy. The 5-year DFS rate was 67.8%, while the 5-year OS rate was 76.4%. The stages were significantly associated with both DFS (P<0.001) and OS (P<0.001), while no significant differences were observed in the DFS (P=0.093) and OS (P=0.399) between the adjuvant chemotherapy followed by EGFR-TKI and adjuvant EGFR-TKI monotherapy groups. A longer duration of EGFR-TKI therapy was associated with better DFS (P<0.001) and OS (P<0.001) benefit. Additionally, pTNM stage and duration of EGFR-TKI therapy were considered independent prognostic factors for long-term survival (All P<0.05).

Conclusions

This study supports the use of EGFR-TKI as a postoperative adjuvant treatment for patients with stage II-IIIA EGFR-mutation positive NSCLC. Additionally, patients with stage I who had pathological risk factors were also suitable for receiving adjuvant EGFR-TKI therapy. Postoperative EGFR-TKI based, chemotherapy-free adjuvant regimen may be a potential therapeutic option for patients with EGFR-mutation positive NSCLC.
Quantifying changes in respiratory syncytial virus—associated hospitalizations among children in Texas during COVID-19 pandemic using records from 2006 to 2021
Authors: Uwak Inyang , Johnson Natalie , Mustapha Toriq , Rahman Mariya , Tonpay Tanaya , Regan Annette K. , Mendoza-Sanchez Itza
Published: March 2023

Aim

To quantify changes on RSV- associated hospitalizations during COVID-19 pandemic, among children four years of age or younger at the state and county levels of Texas using routinely acquired hospital admission records.

Methods

We used the Texas Public Use Data Files (PUDF) of the Department of State Human Services (DSHS) to obtain hospital admissions and healthcare outcomes from 2006 to 2021. We used the 2006–2019 period to estimate a long-term temporal trend and predict expected values for 2020–2021. Actual and predicted values were used to quantify changes in seasonal trends of the number of hospital admissions and mean length of hospital stay. Additionally, we calculated hospitalization rates and assessed their similarity to rates reported in the RSV Hospitalization Surveillance Network (RSV-NET).

Results

An unusually low number of hospitalizations in 2020 was followed by an unusual peak in the third quarter of 2021. Hospital admissions in 2021 were approximately twice those in a typical year. The mean length of hospital stay typically followed a seasonal trend before COVID-19, but increased by a factor of ∼6.5 during the pandemic. Spatial distribution of hospitalization rates revealed localized healthcare infrastructure overburdens during COVID-19. RSV associated hospitalization rates were, on average, two times higher than those of RSV-NET.

Conclusion

Hospital admission data can be used to estimate long-term temporal and spatial trends and quantify changes during events that exacerbate healthcare systems, such as pandemics. Using the mean difference between hospital rates calculated with hospital admissions and hospital rates obtained from RSV-NET, we speculate that state-level hospitalization rates for 2022 could be at least twice those observed in the two previous years, and the highest in the last 17 years.
Population pharmacokinetics of tigecycline in critically ill patients
Authors: Luo Xiangru , Wang Shiyi , Li Dong , Wen Jun , Sun Na , Fan Guangjun
Published: March 2023

Objective: In critically ill patients, the change of pathophysiological status may affect the pharmacokinetic (PK) process of drugs. The purpose of this study was to develop a PK model for tigecycline in critically ill patients, identify the factors influencing the PK and optimiz dosing regimens.

Method: The concentration of tigecycline was measured LC-MS/MS. We established population PK model with the non-linear mixed effect model and optimized the dosing regimens by Monte Carlo simulation.

Result: A total of 143 blood samples from 54 patients were adequately described by a one-compartment linear model with first-order elimination. In the covariate screening analysis, the APACHEII score and age as significant covariates. The population-typical values of CL and Vd in the final model were 11.30 ± 3.54 L/h and 105.00 ± 4.47 L, respectively. The PTA value of the standard dose regimen (100 mg loading dose followed by a 50 mg maintenance dose at q12 h) was 40.96% with an MIC of 2 mg/L in patients with HAP, the ideal effect can be achieved by increasing the dosage. No dose adjustment was needed for Klebsiella pneumoniae for AUC0–24/MIC targets of 4.5 and 6.96, and the three dose regimens almost all reached 90%. A target AUC0–24/MIC of ≥17.9 reached 100% in patients with cSSSI in the three tigecycline dose regimens, considering MIC ≤ 0.25 mg/L.

Conclusion: The final model indicated that APACHEII score and age could affect the Cl and Vd of tigecycline, respectively. The standard dose regimen of tigecycline was often not able to obtain satisfactory therapeutic effects for critically ill patients. For patients with HAP and cIAI caused by one of three pathogens, the efficacy rate can be improved by increasing the dose, but for cSSSI infections caused by Acinetobacter baumannii and K. pneumoniae, it is recommended to change the drug or use a combination of drugs.
Dose adjustment not required for contezolid in patients with moderate hepatic impairment based on pharmacokinetic/pharmacodynamic analysis
Authors: Wu Junzhen , Yang Xinyi , Wu Jufang , Wang Jingjing , Wu Hailan , Wang Yu , Yuan Hong , Yang Huahui , Wang Hailin , Zhang Jing
Published: March 2023

Objective: Contezolid is an oxazolidinone antimicrobial agent newly approved for treatment of Gram-positive bacterial infections. It is primarily metabolized by the liver. This study aimed to assess whether it is required to adjust the dose of contezolid in patients with moderate hepatic impairment for clinicians to use the drug more rationally.

Methods: A single-center, open-label, parallel-group study was conducted to compare the pharmacokinetic (PK) parameters of contezolid and its metabolite M2 between the patients with moderate hepatic impairment and healthy controls with normal liver function after oral administration of 800 mg contezolid tablets. Monte Carlo simulation was performed to calculate the probability of target attainment (PTA) and cumulative fraction of response (CFR) of contezolid based on the PK and pharmacodynamic data.

Results: Oral treatment with 800 mg contezolid tablets was safe and well tolerated in both the patients with moderate hepatic impairment and healthy controls. Moderate hepatic impairment did not result in substantial difference in the area under the concentration-time curve from 0 to 24 h (AUC0–24h, 106.79 vs. 97.07 h μg/mL) of contezolid even though lower maximum concentration (Cmax, 19.03 vs. 34.49 μg/mL) compared with healthy controls. The mean cumulative amount excreted in urine from 0 to 48 h (Ae0–48h) and renal clearance (CLR) of contezolid did not show significant difference between the two groups. Moderate hepatic impairment was associated with lower Cmax, slightly lower AUC and Ae0–48h of M2 compared to the healthy controls. fAUC/MIC was the best PK/PD index to predict the clinical efficacy of contezolid. Monte Carlo simulation results indicated that at the proposed fAUC/MIC target value of 2.3, the dosing regimen of oral contezolid 800 mg q12h could achieve satisfactory PTA and CFR (both >90%) for the target pathogen (methicillin-resistant S. aureus, MIC ≤4 mg/L) in patients with moderate hepatic impairment.

Conclusion: Our preliminary data suggest that dose adjustment is not required for contezolid in patients with moderate hepatic impairment.

Clinical Trial Registration:https://chinadrugtrials.org.cn, identifier: CTR20171377.
Nitrogen fluorescence emission pumped by femtosecond optical vortex beams
Authors: Chen You , Man Xiaoman , Liu Binxian , Lin Zhiyang
Published: March 2023

An experimental investigation on nitrogen fluorescence emissions pumped by a single 800 nm fs optical vortex (OV) beam with different topological charges (TC) is reported. The intensity of the two prototype emission lines from neutral nitrogen molecules (N2) and molecular nitrogen ions (N2+), i.e., 337 nm and 391 nm, respectively, shows different TC dependences: the former slightly decreases as the TC increases, while the latter sharply decreases as the TC changes from zero to a non-zero value. The dependences of the 337-nm and 391-nm emission intensity on pulse energy are also different: the former shows a linear variation for different TCs, while the latter has an abrupt change in the slope when changing the TC. Furthermore, the dependence of the 337-nm emission intensity on gas pressure exhibits a plateau which is universal for different TCs. In contrast, the dependence of the 391-nm emission intensity on gas pressure shows an apparent hump which is respective of the TC. These actual new results will facilitate further theoretical study on the formation dynamics of the nitrogen fluorescence emissions induced by the OV beam, and inspire that such beam can been taken as a unique pure optical tool to manipulate the transitions between different electronic-vibrational states.
A correlation between fractal growth, water contact angle, and SERS intensity of R6G on ion beam nanostructured ultra-thin gold (Au) films
Authors: Jasrotia Priya , Priya Bhanu , Kumar Raj , Bishnoi Priyanka , vij Ankush , Kumar Tanuj
Published: March 2023

Introduction: This study focuses on the detection of rhodamine-6G using surface-enhanced Raman scattering (SERS) on gold nanostructures (AuNS) of different sizes. Ion beam irradiation has been carried out to tune the size of AuNS and investigate the underlying mechanisms of sputtering and diffusion that govern their growth. Additionally, the study established a correlation between fractal growth parameters, water contact angle, and SERS detection of R6G. The results of this study offer new insights into the mechanisms of SERS detection on roughened metallic surfaces.

Methods: Thermal evaporation was used to deposit an Au thin film on a glass substrate. Subsequent 10 keV Ar+ irradiation was done on Au thin film for fluences ranging from 3×1014 to 3×1016 ions/cm2 to tune the size of AuNS. Rutherford backscattering spectroscopy (RBS) was used to confirm that the decrease in Au concentration under ion beam sputtering was responsible for the tuning in size and structure of AuNS. Fractal dimension (Df) and interface width (w) were used as statistical parameters to control the wettable characteristics of the AuNS surfaces.

Results and discussion: The researchers found that the growth of AuNS was governed by ion beam induced sputtering and diffusion mechanisms. They established a correlation between fractal growth parameters, water contact angle, and SERS detection of R6G. They found that a higher surface coverage area of Au NPs with lower fractal dimensions and water contact angles favoured the SERS detection of R6G. This study provides new insights into the mechanisms of SERS detection on roughened metallic surfaces. It is found that the growth of AuNS was governed by ion beam-induced sputtering and diffusion mechanisms, and established a correlation between fractal growth parameters, water contact angle, and SERS detection of R6G. The findings of this study may have applications in the development of more sensitive and efficient SERS-based chemical sensors.
The effect of aquatic exercise on bone mineral density in older adults. A systematic review and meta-analysis
Authors: Schinzel Eileen , Kast Stephanie , Kohl Matthias , von Stengel Simon , Jakob Franz , Kerschan-Schindl Katharina , Kladny Bernd , Lange Uwe , Peters Stefan , Thomasius Friederike , Clausen Jürgen , Uder Michael , Kemmler Wolfgang
Published: March 2023

Introduction: Aquatic or water-based exercise is a very popular type of exercise in particular for people with physical limitations, joint problems and fear of falling. The present systematic review and meta-analysis aimed to provide evidence for the effect of aquatic exercise on Bone Mineral Density (BMD) in adults.

Methods: A systematic literature search of five electronic databases (PubMed/MEDLINE, Cochrane Library, Scopus, Web of Science and CINAHL) according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) was conducted until 2022/01/30, with an update to 2022/10/07. We included controlled trials with a duration of more than 6 months and at least two study groups, aquatic exercise (EG) versus non-training controls (CG) with no language restrictions. Outcome measures were standardized mean differences (SMD) with 95%-confidence intervals (95%-CI) for BMD changes at the lumbar spine (LS) and femoral neck (FN). We applied a random-effects meta-analysis and used the inverse heterogeneity (IVhet) model to analyze the data.

Results: Excluding an outlier study with an exceptionally high effect size for LS-BMD, we observed a statistically significant (p = .002) effect (EG vs. CG) of aquatic exercise for the LS-BMD (n = 10; SMD: 0.30; 95%-CI: 0.11–0.49). In parallel, the effect of aquatic exercise on FN-BMD was statistically significant (p = .034) compared to the CG (n = 10; SMD: 0.76, 95%-CI: 0.06–1.46). Of importance, heterogeneity between the trial results was negligible for LS (I2: 7%) but substantial for FN-BMD (I2: 87%). Evidence for risks of small study/publication bias was low for LS-BMD and considerable for FN-BMD.

Discussion: In summary, the present systematic review and meta-analysis provides further evidence for the favorable effect of exercise on bone health in adults. Due to its safety and attractiveness, we particularly recommend water-based exercise for people unable, afraid or unmotivated to conduct intense land-based exercise programs.
Competitive interactions in two different plant species: Do grassland mycorrhizal communities and nitrogen addition play the same game?
Authors: Bahadur Ali , Jiang Shengjing , Zhang Wei , Sajjad Wasim , Usman Muhammad , Nasir Fahad , Amir Zia Muhammad , Zhang Qi , Pan Jianbin , Liu Yongjun , Chen Tuo , Feng Huyuan
Published: March 2023

In the Tibetan Plateau grassland ecosystems, nitrogen (N) availability is rising dramatically; however, the influence of higher N on the arbuscular mycorrhizal fungi (AMF) might impact on plant competitive interactions. Therefore, understanding the part played by AMF in the competition between Vicia faba and Brassica napus and its dependence on the N-addition status is necessary. To address this, a glasshouse experiment was conducted to examine whether the grassland AMF community’s inocula (AMF and NAMF) and N-addition levels (N-0 and N-15) alter plant competition between V. faba and B. napus. Two harvests took day 45 (1st harvest) and day 90 (2nd harvest), respectively. The findings showed that compared to B. napus, AMF inoculation significantly improved the competitive potential of the V. faba. In the occurrence of AMF, V. faba was the strongest competitor being facilitated by B. napus in both harvests. While under N-15, AMF significantly enhanced tissue N:P ratio in B. napus mixed-culture at 1st harvest, the opposite trend was observed in 2nd harvest. The mycorrhizal growth dependency slightly negatively affected mixed-culture compared to monoculture under both N-addition treatments. The aggressivity index of AMF plants was higher than NAMF plants with both N-addition and harvests. Our observation highlights that mycorrhizal associations might facilitate host plant species in mixed-culture with non-host plant species. Additionally, interacting with N-addition, AMF could impact the competitive ability of the host plant not only directly but also indirectly, thereby changing the growth and nutrient uptake of competing plant species.
Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security
Authors: AHMAD M.
Published: March 2023

Genome editing techniques are being used to modify plant breeding, which might increase food production sustainably by 2050. A product made feasible by genome editing is becoming better known, because of looser regulation and widespread acceptance. The world’s population and food supply would never have increased proportionally under current farming practices. The development of plants and food production has been greatly impacted by global warming and climate change. Therefore, minimizing these effects is crucial for agricultural production that is sustainable. Crops are becoming more resilient to abiotic stress because of sophisticated agricultural practices and a better understanding of the abiotic stress response mechanism. Both conventional and molecular breeding techniques have been used to create viable crop types both processes are time-consuming. Recently, plant breeders have shown an interest in genome editing approaches for genetic manipulation that use clustered regularly interspaced short palindromic repeats (CRISPR/Cas9). To ensure the security of the food supply in the future, plant kinds with desired traits must be developed. A completely new era in plant breeding has begun because of the revolution in genome editing techniques based on the CRISPR/CRISPR-associated nuclease (Cas9) systems. All plants may effectively target a particular gene or group of loci using Cas9 and single-guide RNA (sgRNA). CRISPR/Cas9 can thereby save time and labor compared to conventional breeding methods. An easy, quick, and efficient method for directly altering the genetic sequences in cells is with the CRISPR and Cas9 systems. The CRISPR-Cas9 system, which was developed from components of the earliest known bacterial immune system, allows for targeted gene breakage and gene editing in a variety of cells/RNA sequences to guide endonuclease cleavage specificity in the CRISPR-Cas9 system. Editing can be directed to practically any genomic site by altering the guide RNA (gRNA) sequence and delivering it to a target cell along with the Cas9 endonuclease. We summarize recent CRISPR/Cas9 plant research findings, investigate potential applications in plant breeding, and make predictions about likely future breakthroughs and approaches to food security through 2050.
Genome-wide analysis of TPX2 gene family in Populus trichocarpa and its specific response genes under various abiotic stresses
Authors: Qi Meng , Wang Shengjie , Li Na , Li Lingfeng , Zhang Yue , Xue Jingyi , Wang Jingyi , Wu Rongling , Lian Na
Published: March 2023

Microtubules are essential for regulating cell morphogenesis, plant growth, and the response of plants to abiotic stresses. TPX2 proteins are the main players determining the spatiotemporally dynamic nature of the MTs. However, how TPX2 members respond to abiotic stresses in poplar remains largely unknown. Herein, 19 TPX2 family members were identified from the poplar genome and analyzed the structural characteristics as well as gene expression patterns. All TPX2 members had the conserved structural characteristics, but exhibited different expression profiles in different tissues, indicating their varying roles during plant growth. Additionally, several light, hormone, and abiotic stress responsive cis-acting regulatory elements were detected on the promoters of PtTPX2 genes. Furthermore, expression analysis in various tissues of Populus trichocarpa showed that the PtTPX2 genes responded differently to heat, drought and salt stress. In summary, these results provide a comprehensive analysis for the TPX2 gene family in poplar and an effective contribution to revealing the mechanisms of PtTPX2 in the regulatory network of abiotic stress.
What determines the neural response to snakes in the infant brain? A systematic comparison of color and grayscale stimuli
Authors: Bertels Julie , de Heering Adelaïde , Bourguignon Mathieu , Cleeremans Axel , Destrebecqz Arnaud
Published: March 2023

Snakes and primates have coexisted for thousands of years. Given that snakes are the first of the major primate predators, natural selection may have favored primates whose snake detection abilities allowed for better defensive behavior. Aligning with this idea, we recently provided evidence for an inborn mechanism anchored in the human brain that promptly detects snakes, based on their characteristic visual features. What are the critical visual features driving human neural responses to snakes is an unresolved issue. While their prototypical curvilinear coiled shape seems of major importance, it remains possible that the brain responds to a blend of other visual features. Coloration, in particular, might be of major importance, as it has been shown to act as a powerful aposematic signal. Here, we specifically examine whether color impacts snake-specific responses in the naive, immature infant brain. For this purpose, we recorded the brain activity of 6-to 11-month-old infants using electroencephalography (EEG), while they watched sequences of color or grayscale animal pictures flickering at a periodic rate. We showed that glancing at colored and grayscale snakes generated specific neural responses in the occipital region of the brain. Color did not exert a major influence on the infant brain response but strongly increased the attention devoted to the visual streams. Remarkably, age predicted the strength of the snake-specific response. These results highlight that the expression of the brain-anchored reaction to coiled snakes bears on the refinement of the visual system.
An everlasting love: The relationship of happiness and meaning
Authors: Besika Anastasia
Published: March 2023

Happiness is of great importance to people. Although happiness constitutes a central theme in psychology, the absence of a unifying theory and inconsistent terminology undermine scientific progress. The present article goes beyond attempting to define “types of happiness” or its contributing factors and addresses the role of happiness (i.e., embodied positive emotional patterns) as a function of a dynamic multisystem (i.e., an individual) and its relationship to meaning (i.e., ongoing bidirectional cognitive processes). As a dynamic multisystem, a person strives for stability as they move in physical space, and during their development, across time (i.e., dynamic balance). A primary requirement for dynamic balance is maintaining consistency by connecting the cognitive system to behavior. In psychological terms, such a connection is facilitated by meaning. The model suggests that happiness serves as a marker of a person’s consistency and meaningful interpretations of their lived experience. The model points to a new research direction.
Did smartphones enhance or diminish well-being during the COVID-19 pandemic?
Authors: Heyman Jennifer L. , Kushlev Kostadin
Published: March 2023

Introduction

As smartphones have become increasingly integrated into people’s lives, researchers have attempted to answer whether they are beneficial or detrimental to well-being. Of particular interest to the current study is the role that smartphones played during the first year of the COVID-19 Pandemic.

Methods

In an intensive longitudinal study, we explore how varying uses of smartphones relate to well-being using the Displacement-Interference-Complementarity framework.

Results

Consistent with pre-pandemic research, we show that people felt better, calmer, and more energetic when they used their phones more for complementary purposes (i.e., to access information, entertainment, and connection not otherwise available). In contrast to most pre-pandemic research, however, we find no evidence that any type of phone use predicted lower well-being during the pandemic.

Discussion

Overall, this study lends support to the idea that smartphones can be beneficial for individuals, particularly during times when face-to-face interaction is limited.
Digital twins and the future of precision mental health
Authors: Spitzer Michael , Dattner Itai , Zilcha-Mano Sigal
Published: March 2023

Science faces challenges in developing much-needed precision mental health treatments to accurately identify and diagnose mental health problems and the optimal treatment for each individual. Digital twins (DTs) promise to revolutionize the field of mental health, as they are doing in other fields of science, including oncology and cardiology, where they have been successfully deployed. The use of DTs in mental health is yet to be explored. In this Perspective, we lay the conceptual foundations for mental health DTs (MHDT). An MHDT is a virtual representation of an individual’s mental states and processes. It is continually updated from data collected over the lifespan of the individual, and guides mental health professionals in diagnosing and treating patients based on mechanistic models and statistical and machine learning tools. The merits of MHDT are demonstrated through the example of the working alliance between the therapist and the patient, which is one of the most consistent mechanisms predicting treatment outcome.
The impact of mean body mass index on reported mortality from COVID-19 across 181 countries
Authors: Gabbrielli Ruggero , Pugno Nicola Maria
Published: March 2023

Accountability for global health issues such as a pandemic and its devastating consequences are usually ascribed to a virus, but a comprehensive view should also take into account the state of the host. Data suggests that excessive nutrition is to blame for a yet unknown but not negligible portion of deaths attributed to severe acute respiratory syndrome coronavirus 2. We analyzed the correlation between mean body mass index (BMI) and 2-year coronavirus disease 2019 (COVID-19) mortality rates reported by 181 countries worldwide. Almost two thirds of the countries included had a mean BMI greater or equal to 25, with death rates ranging from 3 to 6,280 per million. Death rates in countries with a mean BMI below 25 ranged from 3 to 1,533. When the analysis was restricted to countries where the extent of testing was deemed more representative of actual mortality, only 20.1% had a mean BMI <25 but the mortality difference persisted. A second analysis looking at pre-vaccination mortality obtained from a different source led to similar conclusions. Due to the nature of the variables, reverse causation can be excluded while common causation can not. A mean BMI <25 for a country seems to spare its citizens from the highest COVID-19 mortality rates. The impact of excess weight on global COVID-19 mortality is suspected to have been much higher than what currently perceived, here estimated at no less than a fourfold increase in mortality. Countries with normal mean BMI constitute precious test beds for the quantification of the effects of overeating on COVID-19 mortality.
Has new rural pension system reduced the intake of junk food among rural older adults? Evidence from China
Authors: Purun Shi , Sun Zhengxiu , Cao Jiaying , Li Zhile
Published: March 2023

At present, China has become one of the fastest growing countries in terms of junk food consumption. However, there has been less previous evidence for the effect of endowment insurance on dietary health. Using the data China Family Panel Studies (CFPS) from 2014, this paper exploits a policy, the New Rural Pension System (NRPS), that only the older adults who have reached 60 years old can receive pensions and conduct a fuzzy regression discontinuity (FRD) to address endogeneity and examine the causal effect of the NRPS on the intake of junk food among rural older adults in China. We find that the NRPS can significantly reduce junk food intake among them, which remains robust after a series of robustness tests. In addition, heterogeneity analysis shows that the female, low-educated, unemployed, and low-income groups are more sensitive to the pension shock from the NRPS. The result of our study provides insights to effectively improve people's dietary quality and related policy formulation.
Virtual nature makes knowledge beautiful
Authors: Harrington Maria C. R.
Published: March 2023

This is a perspective that presents a viewpoint on immersive informal learning applications built as digital twins of the natural world. Such applications provide multimodal, interactive, immersive, embodied, and sensory experiences unique and different from typical game art environments because they are geospatial visualizations of data and information derived from ecological field plot studies, geographical information systems, drone images, and botanically correct 3D plant models visualized in real-time interactive game engines. Since they are constructed from geometric objects, they can programmatically self-express semantic data and connect to knowledge stores on the Internet to create a web of knowledge for both exploration of a virtual environment as a natural landscape and for exploration of connected knowledge stores for informal learning at the moment of curiosity. This design is exceptionally powerful for informal learning as it supports the innate human desire to understand the world. This paper summarizes the construction methods used for creating three digital twins of natural environments and the informal learning applications created and distributed, namely, augmented reality (AR), virtual reality (VR), and virtual field trips. Informal learning outcomes and emotional reactions are evaluated using mixed-methods research studies to understand the impact of design factors. Visual fidelity and navigational freedom are combined to increase learning outcomes and many effective and emotional outcomes as well. Access to facts and story increase learning outcomes, and applications evaluated as beautiful are correlated with emotional reactions of awe and wonder, and awe and wonder are correlated with higher learning gains. Beauty is correlated with other system-wide subjective evaluations largely accepted as important to create a context conducive to support learning outcomes, such as calmness, excitement, and curiosity, a desire to share, and a desire to create. This paper summarizes the highlights of the author’s prior work to give the reader a perspective on the body of work.