bluuebunny/crossref_metadata_2025 huggingface dataset:
Sêrat Bayanullah: Raden Panji Natarata’s Theological Da’wah Rhetoric through Classical Javanese Literature
Authors: Rahmawati Mila Indah , Rais Wakit Abdullah , Wibowo Prasetyo Adi Wisnu
Published: June 2022

This study discusses the wise advice related to Sufism aspects conveyed by Raden Panji Natarata which is packaged in Sêrat Bayanullah based on the character of each têmbang. The purpose of this research is to describe Raden Panji Natarata’s mindset as outlined in the rhetoric of têmbang-têmbang macapat. The method employed descriptive qualitative. The results showed that Raden Panji Natarata was quite skilled at mixing and matching his stories into a da’wah field according to the characteristics of the têmbang. Of the various têmbang macapat presented in this Sêrat Bayanullah, Raden Panji Natarata did not use têmbang durma. This was because têmbang durma has a character that tends to be harsh and angry so that it is not in accordance with the content of the da’wah proclaimed by Raden Panji Natarata. Thus, this locality nuanced da’wah element has an aesthetic and humanistic impression. As the title embedded in this literary work, Sêrat Bayanullah means the explanation of the chapter of Allah. Thus, it is hoped that this literary work can be a reference for understanding the nature of the chapter of Allah.
Believe in Yahweh's Name as God Without Eternal Limitations, God Appears and God Almighty Great
Authors: Budiatmaja Rudy
Published: June 2022

This research aims to provide instructions or guidance from spiritual mentors who believe in Jesus Christ related to how to call, mention the person of God with respect to Jesus Christ (Yahweh) and be worshiped as Savior, which consists of 3 (three) characteristics, namely: (1) the name YHWH is called the Eternal God ('ēl 'ōlam) , (2) the name YHWH is referred to as the visible God ('ēl rō'î) , (3) the name YHWH is referred to as God as the Shepherd for His people ('El shadday) as a giver of life, protection and blessing. The method used is to draw conclusions that are explanatory (explanatory) and confirmatory as well as qualitative in the form of library research using content analysis methods. The results showed that the mention of Yahweh's name is known as the eternal God ('ēl 'ōlam), the God who appears to be always present ('ēl rō'î) and the almighty/powerful God ('El shadday). Finally, it can be concluded that the three names Yahweh or EL are suitable and in accordance with the Hebrew phrases in the Bible and can be trusted for Christians to use them in daily life in facing all challenges or problems of life.
Prejudice and Religious Conflict : Dispositive Analysis of Film “Nama Saya Ahmad”
Authors: Wahyuni Dwi , Nurdin Ahmad Ali
Published: June 2022

So far, studies on the sources of conflict are more dominantly viewed from a macro-social perspective without considering a micro-social perspective. Socio-cultural disparities, political dominance, inequality in economic distribution, and differences in religious or ethnic identity are more dominantly understood as causes of conflict. Whereas the potential for animal power that is inherent in humans is also a factor in the occurrence of conflict. Applying the method of dispositive analysis modeled by S. Jager and F. Maier, this text-based article aims to complete the lack of interpretation of the source of the conflict and analyze how the text of the film “Nama Saya Ahmad” represents the correlation of prejudice and religious conflict. This article shows that the film “Nama Saya Ahmad” depicts religious conflicts starting from prejudice between religious believers. In order for prejudice to be parsed, it is important to expand the dialogue space. Apart from breaking down prejudices, dialogue can also strengthen the social integration of the community.
¿La vuelta a lo sagrado? Secularización, pluralismo religioso y libertad religiosa
Authors: Lázaro-Cantero Raquel
Published: June 2022

El pluralismo religioso actual ha sido clave para revisar tanto el concepto de secularización como el de libertad religiosa. Asistimos a una vuelta a lo sagrado. No se ha cumplido la tesis de la secularización ilustrada: la religión desaparecería de la esfera pública con el progreso de la ciencia. Sin embargo, el resurgimiento religioso significa, de una parte, la presencia en el espacio público de la religión tradicional institucional junto a formas de espiritualidad narcisistas; de otra parte, que es necesario revisar qué se entiende por religión y mostrar el porqué la fe religiosa es un bien que ha de ser protegido jurídicamente.
Eclesialidad y salvación según B.-D. de La Soujeole
Authors: Salgado José-Manuel
Published: June 2022

La consideración de la noción de Iglesia- sacramento ilumina cuestiones como el modo de realización de su sacramentalidad salvífica en el caso de los cristianos no católicos y de los no cristianos. La propuesta de B.-D. de La Soujeole es muy sugerente, en particular en este punto. Partiendo de Cristo como Sacramento y del sacramento de la Eucaristía, propone una eclesialidad participada para todo el que se salva. De esta manera, la necesidad de la Iglesia queda bien situada y se comprende cómo la salvación fuera de sus límites visibles no es una salvación extra Ecclesiam.
High-frequency in vitro shoot regeneration of Saintpaulia ionantha Wendl. by light-emitting diodes
Authors: Bien Le The , Thanh Tung Hoang , Dac Khai Hoang , Manh Cuong Do , Luan Vu Quoc , Ba Nam Nguyen , Thi Huong Trinh , The Vinh Bui Van , Nhut Duong Tan
Published: June 2022

Saintpaulia ionantha Wendl. is a small flower, with a variety of colors, which are commonly grown indoors for decorative purposes. Currently, micropropagation of S. ionantha has been achieved certain results; however, the shoot quality as well as number of shoots regeneration was not high. In this study, the effect of LEDs system on the shoot multiplication and shoot quality of S. ionantha was conducted. The leaves were cut into square patterns without the main veins (1 cm × 1 cm), and the petiole (3 mm in diameter) was cut with a thick layer (2 mm) of longitudinal cell (lTCL), which were used as the initial materials. The explants were placed under LEDs conditions; and white fluorescent tube lamp (FL) was used as the control for shoot regeneration and rooting. The results found that the quality of light affects the regeneration, growth and development of S. ionantha shoots. The LED condition with a combination of 7R + 3B are optimal for high-frequency of shoot regeneration with the regeneration rate: 67.25%, and number of shoots: 9.33 shoots/explant from the leaves without the main veins. The higher shoot multiplication (number of shoots: 10.00 shoots/explant, fresh weight: 150.07 mg, dry weight: 98.64 mg) and rooting rate of plantlet (fresh weight: 2.17 g; dry weight: 0.09 g; leaf number: 20.33 leaves/plantlet) were also obtained under 7R + 3B condition.
Hemagglutinins from the green algae, Chlorophyta
Authors: Dinh Hung Le , Thi Hoai Trinh Phan
Published: June 2022

The extracts from 30 species of the green algae were examined for hemagglutination activity with a variety of different animal and human erythrocytes which were untreated and treated with enzymes. Almost all extracts showed the activity at least toward one type of erythrocytes tested. Strong activity was detected in the extracts from 5 Chlorophyta species (Anadyomene plicata, Avrainvillea erecta, Boodlea struveoides, Halimeda velasquezii and Halimeda discoidea) with enzyme-treated rabbit and sheep erythrocytes. The hemagglutinins of some active species were examined for carbohydrate binding specificity, pH and temperature stability, and effect of divalent cation on their hemagglutination activities. All of the hemagglutinins had no affinity for monosaccharides, except hemagglutinin of Codium arabicum whose activities were inhibited by N-acetyl-D-galactosamine and N-acetyl-D-glucosamine. On the other hand, hemagglutination activities of the hemagglutinins were inhibited by some glycoproteins. The inhibition profiles with glycoproteins were different, depending on hemagglutininproducing species, and suggest the presence of lectins specific for high mannose N-glycans, complex N-glycans, or O-glycans. The hemagglutination activities of the green algal hemagglutinins were stable in a wide range of pH and temperature, and independent on divalent cations. Therefore, Vietnamese green algae may promise as a valuable source of new lectins for application.
Pediatric hepatobiliary disease: Genetic and clinical manifestations
Authors: Lien Nguyen Thi Kim , Anh Hoa Nguyen Pham , Huy Hoang Nguyen
Published: June 2022

In children, hepatobiliary diseases are rarely and mainly due to congenital defects during formation, development of the liver and biliary tract or due to disorders of metabolism. The liver and biliary tract of infant have the incomplete development of physiology during the perinatal until the childhood period. In the process of complete development of the child's liver and biliary tract, there are important changes and affected by genetic and environmental factors. Therefore, the liver and biliary tract are very vulnerable leading to hepatobiliary diseases in children. Disorders in the formation of bile ducts, bile secretion, hepatocellular metabolism, disturbances of metabolism all lead to the formation associated with hepatobiliary diseases. Based on pathophysiology, the hepatobiliary diseases in children can be divided into two groups: hepatobiliary diseases due to the incomplete development of the structure and function of the liver and biliary tract, and hepatobiliary diseases due to the disruption of metabolic processes in liver cells. The secondary effects of hepatobiliary disease can threaten a child's life, and is the cause of metabolic disorders such as hypoglycemia, secondary coagulation disorder due to low concentration of vitamin K-dependent factors leading to intracranial hemorrhage in children, infections caused by immunodeficiency, malnutrition, increased portal venous pressure leading to severe gastrointestinal bleeding... Therefore, the hepatobiliary disease in children should be detected and treated early to avoid adverse complications. In the context of this paper, we focus on hepatobiliary diseases with genetic causes in children. Genetic factors and research situation in the World and Vietnam will be also mentioned. The information about genetic and clinical manifestations will be aggregated to contribute to the general understanding of hepatobiliary diseases in children and to orientate the accurate and effective treatment for patients.
Assessment of reprograming capability of human fibroblast into hepatocyte-like cell by overexpression HNF4α gene in Tet-on system
Authors: Van Hanh Nguyen , Trung Kien Do , Cong Su Trinh , Hai Minh Le Thi , Thu Huong Ngo Thi , Huong Giang Tran Thi
Published: June 2022

This study investigated the ability to reprogramming human fibroblast cells into hepatocyte-like cells by overexpressing HNF4a gene. The fibroblast cells isolated and primary cultured from foreskin donated by healthy patients. The HNF4a gene overexpressed through Doxycilin activatedthe vectors Tet-on-eGFP-HNF4a. The cells after the reprogramming process were evaluated by observing morphological changes, immunofluorescence staining to assess the expression of proteins specific to hepatocytes such as albumin, α-fetoprotein (AFP), HNF4a, and PAS staining to evaluate the ability to glycogen store in cells. The research has isolated and successfully primary cultured fibroblasts. The results showed that after gene transfer, the vector Tet-on-eGFP-HNF4a expressed in all cells and it did not affect the growth rate of the cell. After two weeks, the cells acquired hepatocytes morphology, and they were positive for albumin, AFP, HNF4a and PAS staining. The research has created the cells with some characteristics of liver cells from fibroblasts, however, further studies are needed to comprehensively evaluate them.
Screening and identification of fungi having antimicrobial activity isolated from marine organisms and sediment samples taken in the Bai Tu Long, Vietnam
Authors: Duc Tuan Cao , Thi Quyen Vu , Mai Anh Nguyen , Mai Huong Doan Thi , Van Cuong Pham , Anh Duy Do , Young-Ho Kim  , Hong Lien Hoang Thi , Hong Minh Le Thi
Published: June 2022

Marine ecosystems cover about 70% of the planet surface and are still an underexploitedsource of useful metabolites. Among microbes, filamentous fungi are captivating organisms used for the production of many chemical classes of secondary metabolites used in various fields such as medicine, food ...The present study was focused on the collection, isolation and screening of fungi with antibacterial activity from 25 marine fungi strains isolated by concentration dilution method from 16 samples including marine organisms and sediments collected in Bai Tu Long, Vietnam. The strains were cultured in PDA medium and the culture broths were extracted by ethyl acetate and vacuum rotary evaporation to produce crude extracts. Antimicrobial activity of the extracts was carried out on 7 tested microorganisms, including three Gram-negative bacterial strains (Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Salmonella enterica ATCC13076), three Gram-positive strains (Enterococcus faecalis ATCC29212, Stapphylococus aureus ATCC25923, Bacillus cereus ATCC14579), and yeast Candida albicans ATCC10231. The screening results showed thatfour strains with the highest antibacterial activity (M403, M583, M584 and M612) were capable of inhibiting 4 of the 7 tested microorganisms with minimum inhibitory concentration (MIC) from 32 to 256 µg/mL depending on each tested strain. Specifically, all M403, M583, M584 and M612 inhibited C. albicans ATCC10231 with MIC values ​​of 32-32-64-128 µg/mL, respectively. In addition, all four strains showed inhibitory activity against all three Gram-positive strains tested with MICs from 64 to 256 µg/mL. The strains were identified by morphology and 18S rRNA gene sequences. The results showed that 18S rRNA gene sequences of strains hadover 99% similarity with the 18S rRNA gene sequences available on the Genebank database. Strain M403 was most closely related Aspergillus versicolor and M584 was most closely related Aspergillus unguis, whereas M583 was most closely related Talaromyces purpureogenus and M612 identified as a member Penicillium chrysogenum. The sequences of 18S rRNA gene of four strains were registered on GenBank database with accession numbers: M403 (MW479130), M583 (MW479131), M584 (MW015805) và M612 (MW015801). Preliminary results showed that the marine environment has great potential for isolating fungal strains which contain antibacterial and other bioactive substances.
Antioxidant capacities of the extracts from Perilla frutescens L. leaves using pressurized liquid extraction and conventional techniques
Authors: Bao Thy Vuong , Ngoc Diep Tran , Chi Dung Nguyen , Le Dat Huynh
Published: June 2022

Perilla frutescens L. is an edible plant used as a food item as well as a folk medicine for human diseases in some Asian countries. Due to limited studies in the extraction of total phenols responsible for its high bio-functional properties, this study is aimed to compare the yields, total phenolic content (TPC), and antioxidant capacities of the extracts between pressurized liquid extraction (PLE) at 100°C with or without ultrasonic treatment and conventional extraction techniques. A batch mode of the PLE system was utilized with the use of water and 50% aqueous ethanol as extraction solvents. Hot water extraction at 80°C and soxhlet extraction were employed as conventional techniques. The extraction yields from each technique were determined before their TPC and antioxidant capacities were examined. The results showed that PLE with and without ultrasonic treatment enhanced the extraction process of TPC as compared with conventional ones. Consequently, PLE resulted in higher antioxidant capacities and lipid peroxidation-inhibiting activity than conventional extraction technique (p < 0.05). However, PLE of bioactive components from Perilla frutescens L. leaves with higher thermal treatment should be considered in future studies.
Agarose-paraffin double embedding assisted with 3D printing for high throughput rice root sectioning
Authors: Van Anh Le Thi , Dinh Phuc Hoang , Huong Giang Nguyen , Quynh Trang Pham , Ho Nguyen Le , Mai Quynh Nguyen , Mai Huong To Thi
Published: June 2022

Anatomical analysis of the plant tissues is an important technique used in plant research for studying the process of plant growth, development, adaptation to environmental stress as well as for characterizing gene localization. In Vietnam, most anatomical analysis for plant material was manually conducted which is time-consuming and labor-intensive. In this report, we present an innovative method that can deliver high-quality rice root sectioning. To optimize the efficiency of the conventional paraffin sectioning technique, the agarose-paraffin double embedding was assisted with 3D-printing technique for designing the specific mold which allow to fix from 3 to 12 rice roots in agarose.  Molds consist of a lower section with grooves for firmly holding the rice roots in the right place, the additional middle sections allowing up to 3 layers of root samples to be inserted and an upper section to hold the samples before pouring the embedding. The agarose blocks obtained from the molds were then fixed in the formalin solution, then dehydrated through a gradient concentration of ethanol before being impregnated through a series of mixtures of paraffin/xylene and finally embedded in the paraffin. Anatomical sections of 10 μm of thickness were cut with a microtome, spread on glass slides, and then used for staining and collecting the images. As a result, the agarose-paraffin double embedded tissue blocks were more easily orientated and better solidified for rapid and high-quality sectioning. The sections also showed no interference of agarose with staining, as result, the tissues were much clear. In conclusion, this method represents an innovative technique for high throughput plant sectioning which could be applied to different types of plant tissues.
An in-depth study of substrate effect on the performance of BOD sensor type microbial fuel cell
Authors: Thi My Linh Nguyen , The Hai Pham
Published: June 2022

Nowadays, instant assessment of the organic content in wastewater is an urgent requirement to reduce water pollution. Microbial fuel cells (MFCs) can be used as effective biosensors for rapidly measuring BOD concentration of wastewater. However, wastewaters from different sources may consist of diverse chemical components, which may affect the BOD-measuring performance of MFC-type biosensors. Therefore, in this research, we tested different input substrates for the BOD sensor type MFC (MFC_BOD) to investigate their effects on the performance of the MFC. The substrates belonging to diverse groups such as carbohydrates, organic acids, amino acids and some chlorinated compounds (xenobiotics) were tested at different concentrations equivalent to BOD levels from 10 to 200 mg L-1. Concurrently, we also analyzed the alteration of the bacterial community in the anode of the MFC when tested with those different substrates by using PCR-DGGE. Our results showed that the MFC_BOD could have linear current-to-BOD responses (with the respective R2 values >0.9) to more metabolizable substrates such as carbohydrates, organic acid and glycerol; while it responded less sensitively at different degrees to some amino acids (serine, threonine and methionine) and did not respond to chloroform and chlorobenzene (chlorinated compounds). PCA and bacterial community analysis results surprisingly imply that such different responses may be solely due to different bio(electro)chemical processes associated with the substrates but not due to changes in the composition of the bacterial community. The results suggest that, to enable the MFC_BOD to accurately sense the BODs of the wastewaters containing recalcitrant or toxic substrates, special procedures are required to enrich in the anode the bacterial communities acclimated to the substrates right from the beginning
Analysis of microbial communities in Binh Chau hot spring through metagenomic DNA sequencing
Authors: Thuy Tran Thanh , Lan Nguyen Ngoc , Tung Nguyen Van , Man Tran Dinh , Hoang Nguyen Huy , Thoa Nguyen Kim
Published: June 2022

Binh Chau hot spring locates in Bung Rieng commune, Xuyen Moc district, Ba Ria - Vung Tau province, and is the second hottest hot spring in Vietnam. Up to date, the microbial diversity of this hot spring has been mainly evaluated using the culture-dependent methods, thus, it could not be able to assess all of microorganisms there. In this report, the microbial community of Binh Chau hot spring was investigated by meta-analysis using Hiseq Illumina for sequencing. The results revealed that only 106,903 genes were annotated and 49,190 genes were unknown among 156,093 potential ORFs. The annotated genes consisted of 29,069 bacterial genes, 1,416 archaeal genes, 4 eukaryotic genes, 3 viral genes, and 76,411 unclassified genes. Among the annotated and classified genes, the microbial community of Binh Chau hot spring was characterized by the predominance of bacteria over archaea. At the phylum level, the most abundant phyla in Binh Chau hot spring were followed as Proteobacteria > Firmicutes > Cyanobacteria > Bacteroidetes > Planctomycetes > Thaumarchaeota > Actinobacteria > Euryarchaeota. These belonged mainly to the thermophilic group. A number of the phyla are reported to grow at high temperature, alkaline pH, and high salt concentrations. In addition to the culturable species, the metagenomic data is also indicated the presence of unculturable groups, for example phylum Acidobacteria and Chloroflexi of bacteria. The order Thermoplasmata, which is classified to class Thermoplasmatales of the phylum Euryarchaeota, as well as ultra-small cell and symbiotic archaea including phyla Parvarchaeota, Nanohaloarchaeota, and Nanoarchaeota were detected. The results in this study indicated an important insight into the microbial communities of Binh Chau hot spring, which revealed a promising thermophilic gene sources for their exploitation and conservation in Vietnam.
Efficiency of using probiotic Elac-grow and Han-proway in laying-egg hens
Authors: Van Dinh Thi Tuyet , Thi Thu Nguyen , Hong Linh Nguyen , Thi Men Nguyen , Van Khanh Tran , Nhi Cong Le Thi
Published: June 2022

Industrial chicken farm has to face many challenges such as epidermis and environmental pollution. Antibiotics and synthetic antimicrobial agents are routinely used to alleviate stress as well as to elevate growth and feed efficiency. However, using antibiotic had many negative effects, especially antibiotic resistance; therefore, probiotic products are a bio-secure solution being popularly applied in livestock husbandry.  The study was carried out on the two probiotics   Elac-grow, a diet supplement, and Han-proway, a waste treatment product, in industrial egg-laying hens. There were two lodgings (test and control) in these experiments; each had 4000 23-week-old hens of Hyline breed. Two probiotics were applied to the test and none to the control. The results showed that there was not significantly difference of egg yield between the two groups, although, the test lodging had higher egg yield growing ratio (67.48%) than the control one (65.73%). Egg quality in the experimental lodging was also significantly improved in the damaged egg ratio, the egg weight and the egg yolk weight after using Elac-grow. The efficiency of using Elac-grow and Han-proway was also shown through the concentration of ammonia emission in animal lodging. The ammonia value of the test lodging was maximum 5 ppm and remained stable at this point, lower than of the control. This efficiency was shown significantly in the microbial fluctuation analysis, when the total bacteria and coliform counts were reduced in the test lodging sequentially above 1x108 CFU bacteria/g and above 1x107 CFU coliform/g, the control had uptrend sequentially above 2x109 CFU bacteria/g and above 1x108 CFU coliform/g.
Association study of NR5A1 rs1110061 with infertile male in 401 Vietnamese individuals
Authors: Minh Duc Bui , Lan Anh Luong Thi , Van Hai Nong , Thuy Duong Nguyen
Published: June 2022

Male infertility is a reproductive issue involving defects in the quantity and quality of sperm. Besides the exogenous elements such as harmful habits, pollution, genetic factors including disorders in a single gene, group of genes, or chromosomes, are the etiology causing infertility in men. NR5A1 is well-known as a candidate gene involved in male infertility, including 46, XY disorders of sex development (DSD), cryptorchidism, micropenis, spermatogenic failure, non-obstructive azoospermia, and oligozoospermia. This study aimed to identify the single nucleotide polymorphism (SNP) associated with male infertility in the NR5A1 gene in a Vietnamese cohort of 202 infertile men and 199 healthy controls. By directly sequencing the coding region of the NR5A1 gene in 56 cases and 21 controls, only one missense mutation c.437G>C (p.Gly146Ala; rs1110061) in exon 4 was found. The variant was detected in 34 patients, of which 27 were heterozygous (GC) and 7 were homozygous (CC). In the control group, 15 individuals carried this mutation, including 10 with heterozygous (GC) and 5 with homozygous (CC). To further investigate the association of the polymorphism NR5A1 rs11100061 with male infertility disease, we performed PCR-RFLP in 202 infertile and 199 healthy men to assess the genotypes and alleles. The results indicated that the distribution of the genotypes of the polymorphism was in accordance with Hardy-Weinberg equilibrium (p-values > 0.05). However, no association was detected between the polymorphism and male infertility (p-values > 0.05).
The association of CFAP65 with male infertility in Vietnamese individuals
Authors: Thuy Duong Nguyen , Huu Dinh Tran , Van Hai Nong
Published: June 2022

Cilia- and flagella-associated protein (CFAP) is a well-known protein family that plays a vital role in the spermatogenic process. Recently, the gene CFAP65, which encodes the cilia- and flagella- associated protein 65, has been focused on as a new candidate for male infertility. Mutations in this gene are frequently detected in patients with primary infertility, especially among cases with combined phenotypes of acrosome abnormalities and multiple morphological abnormalities of the flagella (MMAF). In addition, mice carrying both a complete knock-out of the CFAP65 gene and a CFAP65 homozygous frameshift mutation exhibited sterility with the typical phenotypes of MMAF. However, no case-control study has been performed on the relationship between polymorphisms in CFAP65 and male infertility in any population. Hence, our study aimed to investigate the correlation between the polymorphism CFAP65 rs117885048 and male infertility in a Vietnamese population comprising 207 infertile men and 217 healthy controls. As a result, the studied population followed Hardy-Weinberg equilibrium (HWE) (p> 0.05) and the frequencies of genotypes CC/CT/TT were 0.875, 0.12, and 0.003, respectively. The Chi-square test revealed no association between the polymorphism CFAP65 rs117885048 and the disease in this population (p > 0.05). To further interpret the correlation between single nucleotide polymorphisms in the CFAP65 gene and male infertility, a more comprehensive study with other polymorphisms needs to be considered.
A homozygous variant in G6PC in a Vietnamese patient with glycogen storage disease type Ia
Authors: Thu Nguyen Huu Hong , Thi Khanh Ly Nguyen , Thuy Duong Nguyen
Published: June 2022

Glycogen storage diseases (GSDs) are rare inherited metabolic disorders characterized by the absence of required enzymes for the glycogen degradation metabolism. GSD can be divided into more than 12 types based on enzyme deficiency and affected tissues, in which glycogen storage disease type Ia (GSD1a or von Gierke disease) is a liver-affecting form. GSD1a is an autosomal recessive inherited disease caused by mutations in the G6PC gene on chromosome 17q21. The present study reports a Vietnamese family with a 6-month-old male patient diagnosed with type Ia glycogen storage disease. A homozygous variant in the G6PC gene (NM_000151.3: c.518T>C; p.L173P) was detected in the proband using a comprehensive glycogen storage disease panel. This variant has been previously reported in ClinVar (Accession ClinVar: VCV000640818.3). The segregation of the variant was confirmed in ten people of a 3-generation family using Sanger sequencing. The results showed both parents were heterozygous for the variant. In addition, the variant c.518T>C in the G6PC gene was predicted to be deleterious using in silico prediction tools (SIFT, PolyPhen-2, Proven, REVEL, and MutPred2). Our results could help doctors decide on appropriate treatment and diet for the disease. Moreover, the study is also a contribution to molecular studies on GSD1a.
Effects of metal nanoparticles of iron, copper and cobalt on the growth of nitrogen-fixing bacterium Sinorhizobium fredii T14 and nodulation in soybean roots
Authors: Thi Hong Thao Phan , Thi Nhung Dang , Thi Huong Tran , Van Hieu Nguyen , Thi Hong Lien Nguyen , Mai Linh Nguyen Vu , Hong Van Dao Thi , Tuong Van Nguyen , Hoai Chau Nguyen
Published: June 2022

Soybean is a high valuable crop of Vietnam and many other countries. The ability of soybean plants in nitrogen fixation and soil improvement is attributed to their symbiosis with different rhizobia in the root nodules. It has been well documented that members of genus Rhizobium are the most effective in nodulation and nitrogen fixation in legumes. Recently, in oder to minimize undesired accumulation of agro-chemicals and metal salts in soil, nanotechnology products have been intensively studied and applied as nanofertilizers. In this report, the effect of nanoparticles of iron, cobalt and copper on the in vitro growth of nitrogen fixing bacterium Sinorhizobium fredii T14 and nodulation in the root of soybean DT26 was studied. In the cultures of S. fredii T14, nano Fe at the final concentrations of 2, 5, 10, 25 and 50 ppm did not affect the growth, but the latter was reduced of 20, 35 and 46% at 100, 250 and 500 ppm, respectively. No growth inhibition was observed at nano Co concentrations below 10 ppm, while it was reduced of 73% at 500 ppm. Nano Cu seemed to have significant adverse effect on S. fredii T14, showing growth inhibition of 23, 68 and 100% at the concentrations of 5, 25 ppm and 50 ppm, respectively. The production of extracellular polysaccharide by S. fredii T14 was not affected at the concentrations of nano Fe and Co below 250 ppm, while drastic decrease occurred at the presence of nano Cu. Laboratory experiments showed that nanoparticles of all three studied metals at extremely low concentration of 2 ppm significantly increased the number of effective nodules in the roots of soybean DT26 and enhanced the plant growth.
Antifungal potential of silver and copper nanoparticles against Lasiodiplodia theobromae causing stem-end rot of mango
Authors: Vu Mai Linh Nguyen , Hong Thao Phan Thi , Hong Lien Nguyen Thi , Hong Van Dao Thi , Van Hieu Nguyen , Tuong Van Nguyen , Hoai Chau Nguyen
Published: June 2022

Fungal species Lasiodiplodia theobromae of family Botryosphaeriaceae is a pathogen causing significant loss of production and quality of mango crop in Vietnam and worldwide. In this study, a fungal strain designed as XB1 was isolated from stem-end rot mango fruits, identified and tested its susceptibility to silver and copper nanoparticles. Strain identification based on biological characteristics and sequence analysis of 5.8S RNA indicated that the isolate XB1 was highly close (99%) to Lasiodiplodia theobromae, therefore named as Lasiodiplodia theobromae XB1. The inhibiting effects of Ag and Cu nanoparticles on the growth of L. theobromae XB1 were investigated at various concentrations. After seven days of plate cultivation, nano-Cu at 2000 ppm and 4000 ppm inhibited the fungal growth by 17.9 % and 52.4 %, respectively. With silver nanoparticles of about 20 nm in size, the effect seemed rather limited. At 400 ppm, the highest inhibition of 80% was observed after 4 days, then the growth fully resumed. However, another preparation, i.e. nano silver AgH of about 7 nm in size, showed significantly stronger inhibition against the fungus. At 20 ppm, AgH inhibited 90% of growth, comparable to 400 ppm of either carbendazim or hexaconazole. At 25 ppm, no growth was observed. The results suggested high potential of copper and silver nanoparticles AgH to replace toxic chemicals in controlling fungal pathogens of mango and other fruits.
Sulfate/sulfide removal from wastewater by lab-scale microbial fuel cell
Authors: Quynh Hoa Kieu Thi , Minh Hieu Phung , Tuan Anh Vy , Chi Linh Do , Thi Ngoc Quynh Nguyen , Thi Dau Pham , Thi Nga Vuong , Thi Yen Nguyen , Van Giang Nguyen , Xuan Canh Nguyen
Published: June 2022

Sulfate/sulfide-containing wastewater is a widespread environmental contaminant resulting from human activities. These pollutants have negative impact on natural ecosystems and human beings. Biological sulfate/sulfide removal can be achieved by reducing sulfate to sulfide with sulfate-reducing bacteria and then oxidizing sulfide to elemental sulfur (So) with sulfide oxidizing bacteria. In sulfate/sulfide contaminant wastewater lacking electron acceptor for sulfide oxidization, excess sulfide will be produced and accumulated in the treatment system. Therefore, microbial fuel cells (MFCs) have been shown to be a promising technique for the removal of sulfate/sulfide pollutants in wastewater. In this study, a lab-scale MFCs has been developed based on the activity of sulfate-reducing bacterium Desulfovibrio. sp. The results showed that sulfate and sulfide removal efficiencies of 74 - 82% (78 ± 2,5%) and 80.8 - 89.1% (~85,6 ± 3,1%) were achieved, respectively, from the 5th day to the 14th day of operation. The voltage of 0.02V and power density of 7.2 to 7.8 mW/m2 was obtained. In this study, sulfide oxidizing-based MFC integrated with sulfate-reducing bioreactor, representing the feasibility of simultaneous sulfate/sulfide pollutants removal and electricity generation in MFCs. This provides a promising treatment system to scale up for its actual applications in sulfate/sulﬁde removal.
Optimization of fermentation medium for spore production of Paenibacillus polymyxa IN937a and its antifungal activity
Authors: Trung Hieu Pham , Dai Lam Tran , The Tam Le , Thi Thoa Vu , Thi Phuong Thao Nguyen , Tu Cuong Ho , Dinh Hoang Vu , Dang Quang Le
Published: June 2022

In this study, we optimized the fermentation medium for spore production of Paenibacillus polymyxa IN937a. Seven factors including molasses, glucose, magnesium sulfate, potassium pyrophosphate, yeast extract, zinc sulfate, and ammonium sulfate are selected as the basis for the screening of factors affecting the spore production of P. polymyxa IN937a by the Plackett-Burman experiment. Based on the analysis of the Plackett-Burman matrix, the result showed that yeast extract, molasses, and ammonium sulfate were the three main impact factors (P < 0.05), which affected the yield of P. polymyxa IN937a spores. Then, the optimum combination of the three factors was subsequently optimized by the response surface methodology (RSM) using Box-Behnken design to increase the spore production in P. polymyxa IN937a fermentation. The obtained results by RSM predicted that maximum spore density of P. polymyxa IN937a was 6.606×109 spore/mL after 48 hours of the experiment when the appropriate medium for the spore production of P. polymyxa IN937a included yeast extract 14.44 g/L, molasses 19.14 g/L, and ammonium sulfate 0.20 g/L. In addition, the antifungal activity of P. polymyxa IN937a was also tested in this study. The preliminary results of in vitro antifungal activity indicated that P. polymyxa IN937a had a good inhibition on the growth of two phytopathogenic fungal strains Sclerotium rolfsii and Rhizoctonia solani. These results could be used for further research on the fermentation of P. polymyxa IN937a on a pilot scale to obtain the optimal number of spores for use in the development of biological crop protection products.
Comprehending L2 Comprehension: A study of Arabic-Swedish bilingual preschoolers’ performance on a Swedish proficiency test
Authors: Andersson Annika , Lindfors Hanna , Hansson Kristina
Published: June 2022

About a fifth of all children in Sweden learn the societal language Swedish outside of the home, i.e., they have Swedish as a second language (L2). Many of these children have lower socioeconomic status (SES) backgrounds, which predicts lower language proficiency. The aim of the present study is twofold: to contribute to a greater understanding of L2-Swedish proficiency in preschoolers with lower SES backgrounds, and to find out how proficiency tests should be adapted for bilingual children such that the tests are valid, i.e., unbiased to the language status (L1 or L2). We investigate test performance on a Swedish receptive language proficiency test (the Comprehension scale of The New Reynell Developmental Language Scales, NRDLS) which has a monolingual norming sample. The participants are 51 bilingual children (3-5-years of age) with Arabic as their L1, and who attend preschools in Swedish neighborhoods with lower SES. Results indicate that in contrast to the norming sample, bilingual children’s raw scores for subsections of the test are not progressively more difficult. Thus, we need to be aware that bilingual children’s high proficiency in a particular aspect of the language does not necessarily imply that they are proficient in aspects that would be considered easier from a monolingual perspective. In addition, there are indications that unfamiliarity with L2 lexical items, that are typically acquired early in L1, causes bilingual children to fail on tasks aimed at assessing syntactic skills, even though they appear to understand the syntactic pattern. We conclude with suggestions for special considerations and adaptations to assess individual L2-comprehension in preschoolers more accurately, such that practitioners in turn can support the children’s language development.
Strategies for Collaboration Between School and Parents: Homework in General and Translanguaging Homework in Particular
Authors: Svensson Sofia , Donoso Alejandra , Svensson Gudrun
Published: June 2022

The purpose of this case study is to examine if and how working with homework in general and translanguaging homework (TLH) in particular can support collaboration between home and school. Our aim is also to find out if TLH provides the ground for teachers and parents of migrant background to feel and act as epistemic subjects, i.e., as participants whose knowledge is given credibility. Prior research has shown the importance of collaboration between home and school. However, for migrant parents, collaboration has often proved to be problematic and unequal, due to for example language barriers. Research has shown that translanguaging bridges such problems. How the implementation of translanguaging is appreciated by parents and teachers has scarcely been researched. Neither has collaboration through TLH been studied regarding how they perceive each other as epistemic subjects. In this study, thematic content analysis has been applied on interview data with parents and teachers. The epistemic (in)justice framework has then been used to discuss the found themes in their answers. The findings show that teachers’ and parents’ perceptions of working with TLH differs. Some parents perceive that TLH enhances their chances to collaborate with school as epistemic subjects. Conversely, TLH may obscure other parents’ possibilities to collaborate, thus creating epistemic injustice. Lastly, TLH might be ignored altogether by some parents, thus neither enhancing collaboration nor creating epistemic justice. Concludingly, in order for TLH to work, reciprocal sensitive listening to each other, from both the teachers’ and the parents’ side, is important, and teachers need to be aware of the students’ family situation regarding their language choices and language use.
Elever i ett språkligt heterogent klassrum rör sig mot naturvetenskapliga beskrivningar av ljud
Authors: Uddling Jenny , Danielsson Kristina
Published: June 2022

Den här studien belyser hur ett ämnesinnehåll bearbetas i ett naturvetenskapligt klassrum där flera elever undervisas på sitt andraspråk. Närmare bestämt undersöks om och i så fall hur eleverna i ett språkligt heterogent fysikklassrum i årskurs åtta, i samtal mellan lärare och elever samt mellan elever, ges stöd i att utveckla en allt mer naturvetenskaplig beskrivning av ljud.
De data som ligger till grund för studien består av video- och ljudinspelningar, digitala foton och insamlade texter från fyra fysiklektioner. Analysen grundas i socialsemiotisk teori (Halliday 1978), med verktyg från systemisk-funktionell grammatik (SFG) i syfte att identifiera tecken på lärande i elevernas uttryck.
Resultatet visar att läraren iscensatte aktiviteter som bidrog till elevernas möjligheter att utveckla kunskaper om ämnesinnehållet och att uttrycka sig om ljud på ett alltmer ämnesadekvat vis. Emellertid gav några av de språkliga resurser som läraren erbjöd inte det stöd som kanske var avsett. Studien har implikationer för hur lärande i språkligt heterogena natur-vetenskapliga klassrum kan främjas, där en central aspekt är att lärare på ett medvetetet sätt själva använder språket och att de resonerar med eleverna om hur detta bidrar till att uttrycka ämnesinnehållet.
Main Directions and Principles of Applying Technology 4.0 by Foreign Trade Companies of the EAEU and BRICS Jurisdictions
Authors:   Volgograd State University, Goncharov Alexander , Goncharova Marina ,   Volgograd Institute (Branch) of Plekhanov Russian University of Economics
Published: June 2022

Introduction: the paper reveals a scientific and methodological approach to determining the main (priority) directions of development and the principles of application of digital technologies of Industry 4.0, which increase efficiency and should be applied by economic entities of the EAEU and BRICS jurisdictions in the implementation of foreign trade activities. The purpose of the study is to reveal the main directions and principles of the application of 4.0 technologies by foreign trade companies of the EAEU and BRICS jurisdictions. Methods: the research is carried out based on the materialistic worldview of the authors and the universal scientific method of historical materialism. The general scientific and specific scientific methods of cognition are applied. Results: two priority main directions of development are substantiated; five fundamental principles of the application of the digital technologies of Industry 4.0 are identified, contributing to the proper implementation of cross-border export-import transactions between residents of the member states of integration associations with the participation of the Russian Federation, as well as the prevention of potential conflicts and economic disputes. Conclusions: the authors substantiate the proposal to digitize the space-application of neo-industrial digital technologies 4.0 by foreign trade companies of the EAEU and BRICS member states in the form of an online portal “Foreign Economic Activity of the Greater Eurasian Partnership online”, on which each participant in foreign economic activity will be able to open and operate his own legally significant personal account. At the same time, it is proposed to fix the priorities and principles of the development of the space for the use of neo-industrial digital technologies 4.0 by foreign trade companies of the member countries of the Greater Eurasian Partnership (EAEU + BRICS) in an interstate conventional agreement.
Political and Legal Aspects of the Protection of Constitutional Social Rights of Citizens in Russia
Authors:   Volgograd Interdistrict Bar Association, Ushakov Alexey
Published: June 2022

Introduction: the paper analyzes the legal features of the formation of the legal positions of the European Court of Human Rights and the Constitutional Court of the Russian Federation in the field of protection of social rights of citizens in the Russian Federation. As the practice of law enforcement and human rights activities shows, the constitutional social rights of Russian citizens are most often violated. For this purpose, the court rulings issued in the field of protection of social rights by citizens have been studied. Using the methods of scientific cognition, first of all, the system analysis of judicial and regulatory legal acts, the peculiarities of the formation of legal positions of judicial legal instances of the Russian and international level are revealed. Results: the study has revealed the existence of controversial issues in the formulation of legal positions of the interstate human rights body and the Constitutional Court of the Russian Federation. Conclusions: during the formulation of legal positions by the Constitutional Court of the Russian Federation, the principles of protection of social rights of citizens are formulated; however, when making decisions by the European Court of Human Rights, a political aspect is often manifested.
Conceptual and Methodological Foundations of the Interpretation of Legal Nihilism
Authors:   Vitebsk State University named after P.M. Masherov, Bochkov Alexander , Sukharev Andrey ,   Vitebsk State University named after P.M. Masherov
Published: June 2022

Introduction: the paper considers the conceptual and methodological interpretation of legal nihilism as a complex interdisciplinary and intersectoral phenomenon. The purpose of the study is to show legal nihilism as a result of the deformation of state-legal reality and to identify ways to combat it. The objectives of the work are to give a terminological characterization of legal nihilism as a dialectically contradictory integral socio-cultural phenomenon; to identify its nature, meaning, forms, and causes generating consequences. Methods: the convergent methodology combining general, general scientific and specific scientific research methods. The main methods are the methods of dialectical materialism, the synergetic, interdisciplinary methods, analysis, synthesis, interpretation of the law, comparative studies. Results: legal nihilism as a direction of socio-political thought and legal practice is a catastrophogenic factor of society, due to its mass character, oppositional power, and destructiveness. The authors believe that the results and consequences can be foreseen, they need to be managed with the help of information and communication technologies, artificial intelligence. The hypothesis of the natural “predisposition” of Russians and Belarusians to legal nihilism has not been confirmed, however, it has shown a historical, national, cultural peculiarity of the perception of state and law. Conclusions: the growth of the omnipotence of the state in the conditions of an external military threat, sanctions, and pandemics leads to the growth of legal nihilism at all levels of social reality. The way out is to improve objective and subjective factors related to the legality, legitimacy, social orientation of the state, social cohesion, the improvement of ideological, patriotic, civil, legal education. It is required to develop doctrinal, legislative, law enforcement aspects of the problem at the state level; to create a digital monitoring mechanism for the objective, representative determination of the level of legal nihilism in society and a list of urgent measures to overcome negative consequences.
Elements of Myth in David Almond’s Fiction
Authors:   Linguistics University of Nizhny Novgorod, Razina Irina S.
Published: June 2022

The study considers how motifs and plots borrowed from mythology function in David Almond’s fiction for children. Almond uses them to analyze the fundamental concepts of love and death, as well as to discuss teenage sexuality. He highlights the role of creative activities in teen identity development. Myth is also incorporated into descriptions of children undergoing rites of passage.