bluuebunny/crossref_metadata_2025 huggingface dataset:
Environmental Nanoparticles Reach Human Fetal Brains
Authors: Calderón-Garcidueñas Lilian , Pérez-Calatayud Ángel Augusto , González-Maciel Angélica , Reynoso-Robles Rafael , Silva-Pereyra Héctor G. , Ramos-Morales Andrea , Torres-Jardón Ricardo , Soberanes-Cerino Candelario de Jesús , Carrillo-Esper Raúl , Briones-Garduño Jesús Carlos , Conde-Gutiérrez Yazmín del Socorro
Published: February 2022

Anthropogenic ultrafine particulate matter (UFPM) and industrial and natural nanoparticles (NPs) are ubiquitous. Normal term, preeclamptic, and postconceptional weeks(PCW) 8–15 human placentas and brains from polluted Mexican cities were analyzed by TEM and energy-dispersive X-ray spectroscopy. We documented NPs in maternal erythrocytes, early syncytiotrophoblast, Hofbauer cells, and fetal endothelium (ECs). Fetal ECs exhibited caveolar NP activity and widespread erythroblast contact. Brain ECs displayed micropodial extensions reaching luminal NP-loaded erythroblasts. Neurons and primitive glia displayed nuclear, organelle, and cytoplasmic NPs in both singles and conglomerates. Nanoscale Fe, Ti, and Al alloys, Hg, Cu, Ca, Sn, and Si were detected in placentas and fetal brains. Preeclamptic fetal blood NP vesicles are prospective neonate UFPM exposure biomarkers. NPs are reaching brain tissues at the early developmental PCW 8–15 stage, and NPs in maternal and fetal placental tissue compartments strongly suggests the placental barrier is not limiting the access of environmental NPs. Erythroblasts are the main early NP carriers to fetal tissues. The passage of UFPM/NPs from mothers to fetuses is documented and fingerprinting placental single particle composition could be useful for postnatal risk assessments. Fetal brain combustion and industrial NPs raise medical concerns about prenatal and postnatal health, including neurological and neurodegenerative lifelong consequences.
α1-Acid Glycoprotein-Decorated Hyaluronic Acid Nanoparticles for Suppressing Metastasis and Overcoming Drug Resistance Breast Cancer
Authors: Omar Haneen , Fardous Roa’ , Alhindi Yasser M. , Aodah Alhassan H. , Alyami Mram , Alsuabeyl Mohammed S. , Alghamdi Waleed M. , Alhasan Ali H. , Almalik Abdulaziz
Published: February 2022

Robust inflammation-suppressing nanoparticles based on α1-acid glycoprotein (AGP)-conjugated hyaluronic acid nanoparticles (AGP-HA NPs) were designed to regulate breast cancer cells’ sensitivity to chemotherapy and to suppress tumor metastasis. The successful conjugation between AGP and HA NPs was confirmed using FTIR, zeta potential, and UV–vis spectroscopy. In vitro studies on MCF-7 cells indicated the remarkable ability of AGP-HA NPs in suppressing migratory tumor ability by 79% after 24 h. Moreover, the efficacy study showed the high capability of AGP-HA NPs in modulating MDA-MB-231 cells and restoring cell sensitivity to the chemotherapeutic drug doxorubicin (DOX). Furthermore, the finding obtained by flow cytometry and confocal spectroscopy demonstrated that AGP-HA NPs enhanced DOX uptake/retention and aided it to reach cell nucleus within 4 h of incubation. Therefore, AGP-HA NPs represent a viable and effective treatment option to strengthen the anticancer effects of chemotherapeutic agents and potentially improve patients’ survival rates.
HPRT1 Promotes Chemoresistance in Oral Squamous Cell Carcinoma via Activating MMP1/PI3K/Akt Signaling Pathway
Authors: Wu Tong , Jiao Zan , Li Yixuan , Su Xuan , Yao Fan , Peng Jin , Chen Weichao , Yang Ankui
Published: February 2022

Hypoxanthine phosphoribosyl transferase 1 (HPRT1) is traditionally believed to be a housekeeping gene. However, recent reports have indicated that HPRT1 overexpression is associated with a poor prognosis in various types of cancers. Using The Cancer Genome Atlas (TCGA), HPRT1 was found to be highly expressed in various cancer types, especially in head and neck squamous cell carcinoma (HNSCC). Therefore, we measured HPRT1 expression in human cancer tissues and adjacent non-carcinoma tissues (ANT) and explored the relationship between HPRT1 expression and clinical pathological factors and prognosis in patients with oral squamous cell carcinoma (OSCC), a common type of HNSCC. We built OSCC cells with stable knockdown and overexpression of HPRT1 to observe its influence on chemoresistance and malignancy in vitro and vivo. We found that highly expressed HPRT1 was associated with a poor prognosis and could promote resistance to cisplatin (CDDP) in OSCC cells in both in vitro and in vivo. An RNA sequence assay was carried out to explore the mechanism of function of HPRT1, we found that HPRT1 could positively regulate the expression of MMP1 and the activation of the PI3K/AKT pathway, to regulate the resistance to CDDP of OSCC. In conclusion, HPRT1 can no longer be simply believed to be a housekeeping gene. HPRT1 overexpression indicates a worse prognosis and can improve CDDP resistance for patients with OSCC by promoting the MMP1/PI3K/Akt axis. HPRT1 may be a potential prognostic biomarker and therapeutic target in OSCC.
Evaluation of a Proposal for Movement Integration in the Teaching–Learning Process in Early Childhood Education
Authors: Nielsen-Rodríguez Adriana , Romance Ramón , Dobado-Castañeda Juan Carlos , Gil-Espinosa Francisco Javier
Published: February 2022

Physical activity is essential to child development, but studies show that children are increasingly inactive. Due to schools being considered privileged environments to promote physical activity, the aim of this study was to increase the physical activity performed by early childhood education children during the school day by integrating movement into academic content and analyze this process. The amount and intensity of physical activity performed by a group of 24 3–4-year-old children in three different weeks were measured by accelerometry: one week with the methodology they had been following (week 1); and two weeks in which movement was integrated into the content through a specific proposal (week 2) and the same improved proposal (week 3). The results reveal that the application of a movement integration program not only allowed students to work on academic content in a physically active way, but also significantly increased the amount of physical activity that children performed during the school day. However, it was necessary to carry out several interventions the same day, or make movement integration the reference methodology, to meet the minimum recommended physical activity levels. In addition, to increase their effectiveness, interventions should be continually reviewed and improved to increase the motor engagement time.
Does Early Treatment Improve Clinical Outcome of Class II Patients? A Retrospective Study
Authors: Männchen Roland , Serafin Marco , Fastuca Rosamaria , Caprioglio Alberto
Published: February 2022

The present study was carried out to evaluate the benefits from one-phase Class II Early Treatment (ET) using extraoral forces and functional appliances but without intermaxillary forces and eventual lower leeway space preservation compared to two-phase Class II Late Treatment (LT) with the need for extractions and full fixed appliances as well as lower incisor proclination. The ET group (n = 239, 115 M, 124 F, mean age 10.6 ± 1.2 years), with first premolars not in contact and the second deciduous lower molars preserved, was compared to the LT group (n = 288, 137 M, 151 F, mean age 12.4 ± 1.5 years). The ET group was first treated with headgears, growth guide appliances, or Teuscher activators and, in borderline crowding cases, with lower space maintenance by a lingual arch, lip bumper, or fixed utility arch. The LT group and the second phase of ET were treated with full fixed appliances including intermaxillary forces such as Class II elastics or noncompliance devices; headgear and a growth guide appliance were also used. Cephalograms and plaster models were taken before (T1) and after treatment (T2) to calculate cephalometric changes and space balance discrepancies. The differences between T1 and T2 were analyzed by a t-test for normally distributed data and by the Mann–Whitney Test for nonnormally distributed data at a level of p < 0.05. The groups were defined as statistically homogeneous at T1. A statistical analysis showed that the ET group (mean treatment time 35.3 ± 13.3 months) was significantly associated with a 22.2% lower extraction rate, 15.9% less need for a full fixed appliance, and more than 5° less incisor proclination in the nonextraction cases compared to the LT group (mean treatment time 25.9 ± 8.1 months); treatment time significantly increased in the ET group compared to the LT group. Early Class II treatment resulted in a significant treatment effort reduction in more than one third of the patients and less lower incisor proclination, even if it clinically increased treatment time.
A Social Exploration of the West Australian Gorgon Gas, Carbon Capture and Storage Project
Authors: Marshall Jonathan Paul
Published: February 2022

Carbon capture and storage (CCS) appears to be essential for lowering emissions during the necessary energy transition. However, in Australia, it has not delivered this result, at any useful scale, and this needs explanation. To investigate the reasons for this failure, the paper undertakes a historical and social case study of the Gorgon gas project in Western Australia, which is often declared to be one of the biggest CCS projects in the world. The Gorgon project could be expected to succeed, as it has the backing of government, a practical and economic reason for removing CO2, a history of previous exploration, nearby storage sites, experienced operators and managers, and long-term taxpayer liability for problems. However, it has run late, failed to meet its targets, and not lowered net emissions. The paper explores the social factors which seem to be disrupting the process. These factors include the commercial imperatives of the operation, the lack of incentives, the complexity of the process, the presence of ignored routine problems, geological issues (even in a well-explored area), technical failures, regulatory threats even if minor, tax issues, and the project increasing emissions and consuming carbon budgets despite claims otherwise. The results of this case study suggest that CCS may work in theory, but not well enough under some contemporary forms of social organisation, and the possibilities of CCS cannot be separated from its social background. Social dynamics should be included in CCS projections to enhance the accuracy of expectations.
The Theoretical Approach to the Modelling of Gully Erosion in Cohesive Soil
Authors: Sidorchuk Aleksey
Published: February 2022

The stochastic gully erosion model (STOGEM) is based on a combination of deterministic mechanics and a stochastic description of the erosion control factors. The main proposition in the model is that the depth of the active surface layer of eroded cohesive soil is equal to one particle diameter, and the deposition of eroded particles is negligible. The erosion rate at the gully bed is calculated directly from the equation of the balance between driving and resistance forces acting on soil particles in flowing water using the probability density functions of stochastic variables: flow velocity, soil aggregate size and cohesion. Probability density functions of cohesion in the model vary through time and space during the erosion event due to the changes in soil composition—armoring and loosening. This theory is still far from achieving practical application, but opens up a new way for better understanding the experimental results of gully erosion and shows the direction for future investigations.
Linking Sexting Expectancies with Motivations to Sext
Authors: Currin Joseph M.
Published: February 2022

While many researchers have explored the impact sexting may have on relationships and mental health, few have explored the motivations and expectancies as to why individuals engage in sexting. By understanding why individuals sext their partners, we can learn more about what drives the behavior. Therefore, the current study sought to determine if sexting for sexual purposes (SP) or body image reinforcement (BIR) would predict positive sext expectancies. There was no prediction for instrumental/aggravated reasons (IAR). The online questionnaire had 348 participants, and based on regression analysis, positive sext expectancies while sending a sext message predicted sexting for sexual purposes. Somewhat surprisingly, sexting for instrumental/aggravated reasons was predicted by negative sext expectancies (both sending and receiving). These findings demonstrate individuals who sext for sexual purposes, and have positive sext expectancies, appear to enjoy the consequences of that behavior. Individuals who sext for instrumental/aggravated reasons may be uncomfortable with the outcome of their sexting behavior. This result highlights an area where clinicians could help clients explore the true reinforcements behind IAR.
Degrowth Perspective for Sustainability in Built Environments
Authors: Nesterova Iana
Published: February 2022

Degrowth, as a social movement, a political project, and an academic paradigm, aims to find ways that can lead to harmonious co-existence between humanity and nature, between humans and non-humans, and within humanity, including oneself. Seen through the lens of degrowth, everything becomes subject to reflection, critique, re-evaluation, and re-imagining. This concerns environments created by humans in a long process of interaction with nature, i.e., built environments. Built environments are always in becoming. This entry contemplates the implications of degrowth for intentionally directing this becoming towards genuine sustainability.
A Model Predictive Control-Based Decision-Making Strategy for Residential Microgrids
Authors: Mechleri Evgenia , Dorneanu Bogdan , Arellano-Garcia Harvey
Published: February 2022

This work presents the development of a decision-making strategy for fulfilling the power and heat demands of small residential neighborhoods. The decision on the optimal operation of a microgrid is based on the model predictive control (MPC) rolling horizon. In the design of the residential microgrid, the new approach different technologies, such as photovoltaic (PV) arrays, micro-combined heat and power (micro-CHP) units, conventional boilers and heat and electricity storage tanks are considered. Moreover, electricity transfer between the microgrid components and the national grid are possible. The MPC problem is formulated as a mixed integer linear programming (MILP) model. The proposed novel approach is applied to two case studies: one without electricity storage, and one integrated microgrid with electricity storage. The results show the benefits of considering the integrated microgrid, as well as the advantage of including electricity storage.
Effectiveness of the Food-Safe Anaesthetic Isobutanol in the Live Transport of Tropical Spiny Lobster Species
Authors: Pozhoth Jayagopal , Jeffs Andrew
Published: February 2022

The strong demand for live spiny lobsters in Asian markets is being met by long-distance airfreight. Holding lobsters out of water during transportation often results in mortalities due to the accumulation of metabolites, especially ammonia. This study examined the potential to improve the survival of tropical lobster species exported from India through the use of the food-safe aquatic anaesthetic isobutanol, both with and without cold stunning, typically used prior to live lobster transportation. The results of the study indicate that treatment with 50 ppm isobutanol in ambient seawater temperature (i.e., 28 °C) prior to simulated live transport for 22 h significantly reduces ammonia levels in the haemolymph in all four lobster species (Panulirus homarus, P. ornatus, P. versicolor and P. polyphagus) compared to lobsters treated with cold stunning (i.e., 16.5 °C) with and without 10 ppm isobutanol. Cold stunning at 16.5 °C combined with 10 ppm isobutanol reduced ammonia levels compared to cold stunning alone only in P. ornatus. All experimental lobsters were returned to ambient seawater after simulated transport and were alive after 48 h. These results indicate that isobutanol has the potential to be used to suppress metabolism during the live transport of tropical lobsters and to reduce mortalities during live transport.
Fatty Acid Profiling in Kernels Coupled with Chemometric Analyses as a Feasible Strategy for the Discrimination of Different Walnuts
Authors: Pei Qiao , Liu Yongxiang , Peng Shaobing
Published: February 2022

Walnuts have high oil content in their kernels, and they have attracted considerable attention in the food, beverage, nutrient, and health fields because of their delicious taste and potential health benefits. Fatty acid profiles of kernels vary depending on walnuts species, ontogenic variations, and planting environments. To determine the key indicators that can be used to distinguish different walnuts using chemometric analyses, the fatty acid compositions and contents of 72 walnut samples were measured and evaluated. Three fatty acids, oleic acid (21.66%), linoleic acid (56.40%), and linolenic acid (10.50%), were the most common fatty acid components in the kernels. Palmitic acid and linolenic acid in kernels were found to be indicators to rank the walnuts into five levels. Three groups were identified based on of several chemometrics. Oleic acid in kernels was typical fatty acid that could be used to distinguish three walnut groups based on the results of discriminant analysis, while oleic acid and linoleic acid were key differential fatty acids on the discrimination of each group based on the result of orthogonal partial least squares discriminant analysis. This study provides information on how to classify walnuts from different geographical locations based on kernel fatty acid profiling and provides an approach to identify possible adulterations in walnuts on the markets. Moreover, the results are potentially relevant to quality assessments of walnuts.
Hydrogels on the Base of Modified Chitosan and Hyaluronic Acid Mix as Polymer Matrices for Cytostatics Delivery
Authors: Vildanova Regina , Lobov Alexander , Spirikhin Leonid , Kolesov Sergey
Published: February 2022

The development of biodegradable polysaccharide hydrogel matrices for cytostatic delivery can improve the therapeutic results of patients by prolonging the action of the drug, reducing its toxicity and providing additional biological activity by polysaccharides. In this work, N-succinyl chitosan/hyaluronic acid dialdehyde/cytostatic formulations have been prepared using two different chitosan grades (30 kDa and 150 kDa) and hyaluronic acid dialdehyde. The interaction of amino groups of N-succinyl chitosan and aldehydes of hyaluronic acid resulted in the formation of azomethine bonds and was demonstrated using 13C NMR. The elastic properties of the obtained hydrogels determine their use as implants. Two cytostatics—5-fluorouracil and mitomycin C were chosen as drugs because of their using both in oncology and in ophthalmology for the surgical treatment of glaucoma. Hydrogel formulations containing cytostatic were prepared and drug release was studied using in vitro dialysis method. It was established that the molecular weight of N-succinyl chitosan and rheological properties of hydrogel influenced the drug release behavior of the gelling delivery system. Formulations prepared from N-succinyl chitosan with greatest molecular weight and mitomycin C were found to be the most promising for medical application due to their rheological properties and prolonged drug release. Mild preparation conditions, simplicity of the technique, short gelation time (within a minute), 100% yield of hydrogel, suitability for drug release applications are the main advantages of the obtained hydrogels.
Study of the Interfacial Transition Zone Characteristics of Geopolymer and Conventional Concretes
Authors: Alanazi Hani
Published: February 2022

The properties and performance of geopolymer at different length scales have been intensively studied, but only limited studies on geopolymer have investigated the zone located between paste and aggregates, which is called the interfacial transition zone (ITZ). The microstructure of ITZ and its nanomechanical properties in geopolymer concrete are examined in this study. Fly ash-based geopolymer has great potential to be an alternative to traditional concrete. To this end, scanning electron microscopy (SEM) and nanoindentation tests were performed to investigate the microstructural characteristics and nanomechanical properties of the ITZ, and the results were compared with the ITZ of traditional concrete. Results show that traditional concrete demonstrated a weak ITZ with pores and microcracks, while the geopolymer concrete microstructure did not present weak ITZs in the vicinity of aggregates. More pores and crack were observed in the ITZ in traditional concrete. Further, a considerable amount of fly ash particles, that appear to be unreacted or partially reacted in the matrix phase, was observed. Based on the nanoindentation results, 58% of the microstructure is composed of unreacted or partially reacted fly ash particles. The results of nano- and microscale tests will enhance the understanding of how concrete behaves and performs at large scales.
Lipid Biomarkers in Depression: Does Antidepressant Therapy Have an Impact?
Authors: Stuchtey Fidelis Christin , Block Andrea , Osei Francis , Wippert Pia-Maria
Published: February 2022

Studies have revealed mixed results on how antidepressant drugs affect lipid profiles of patients with major depression disorder (MDD). Even less is known about how patients respond to a switch of antidepressant medication with respect to their metabolic profile. For this, effects of a switch in antidepressants medication on lipid markers were studied in MDD patients. 15 participants (females = 86.67%; males = 13.33%; age: 49.45 ± 7.45 years) with MDD and a prescribed switch in their antidepressant medication were recruited at a psychosomatic rehabilitation clinic. Participants were characterized (with questionnaires and blood samples) at admission to the rehabilitation clinic (baseline, T0) and followed up with a blood sample two weeks (T1) later. HDL, LDL, total cholesterol, and triglycerides were determined (T0), and their change analyzed (Wilcoxon test) at follow up (T1). Decrements in HDL (p = 0.041), LDL (p < 0.001), and total cholesterol (p < 0.001) were observed two weeks after a switch in antidepressant medication. Triglycerides showed no difference (p = 0.699). Overall, LDL, HDL, and total cholesterol are affected by a change in antidepressant drugs in patients with MDD. These observations are of clinical relevance for medical practitioners in the planning and management of treatment strategies for MDD patients.
Accurate Quantification of Anthocyanin in Red Flesh Apples Using Digital Photography and Image Analysis
Authors: Grimm Eckhard , Kuhnke Felix , Gajdt Anna , Ostermann Jörn , Knoche Moritz
Published: February 2022

Red fleshed apples (Malus × domestica Borkh.) differ in colour intensity between cultivars, seasons and sites. The objective of this study was to develop a procedure for predicting anthocyanin content from digital images of flesh discs. Flesh cylinders of uniform colour were excised, scanned and their colours determined in the R, G and B and the L*a*b* colour spaces. Anthocyanin content was also quantified chemically. A calibration line was constructed to predict anthocyanin content of flesh discs of varying colour from a scan or a photograph in the studio or outdoors. Anthocyanin concentration was linearly related to the logarithms of G, B and L*. From these relationships, the anthocyanin content of a flesh disc was predicted, pixel by pixel. Colour corrections were applied using a reference colour chart included in all images. The Finlayson algorithm was most effective for correcting the G parameter obtained by a flatbed scanner. For variable imaging methods (scanning or photography), the Vandermonde algorithm for correcting the L* parameter and the Finlayson algorithm for correcting the G parameter were most effective in predicting anthocyanin content. The procedure allows accurate prediction of anthocyanin content of red fleshed apples from simple colour scans or photographs.
Breeding, Genetics, and Genomics of Ornamental Plants
Authors: Bhattarai Krishna , Van Huylenbroeck Johan
Published: February 2022

Ornamental crops include a broad range of plants, including herbaceous seasonal flowers to woody perennial trees [...]
Analytical Validation of Familial Hypercholesterolemia Biomarkers in Dried Blood Spots
Authors: Held Patrice K. , Campbell Kristin , Wiberley-Bradford Amy E. , Lasarev Michael , Horner Vanessa , Peterson Amy
Published: February 2022

Heterozygous familial hypercholesterolemia (HeFH) is a common, treatable genetic disorder characterized by premature atherosclerosis and cardiovascular disease, yet the majority of affected individuals remain undiagnosed. Newborn screening could play a role in identification of at-risk individuals and provide an opportunity for early intervention, prior to the onset of symptoms. The objective of this study was to develop and validate assays for quantification of candidate HeFH biomarkers in dried blood spots (DBS). Commercially available enzyme assay kits for quantification of serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) were modified for high-throughput analysis of DBS. Apolipoprotein B (ApoB) concentrations in DBS were measured using an immunoassay with modifications from published studies. All three assays were validated according to the College of American Pathologists guidelines for clinical laboratories. The performance of TC, LDL-C, and ApoB assays was assessed by precision, recovery, limit of quantification (LOQ) and linearity. Precision studies yielded coefficients of variation (CV) of less than 15%, with recovery greater than 75% for all three assays. The determined LOQ and linearity were comparable to serum-based assays. In a direct comparison between serum and DBS concentrations, positive correlations were demonstrated for TC, LDL-C, and ApoB. Additionally, the initial evaluation of the three biomarker concentrations within the unaffected population was similar to values obtained in previous published studies. This study reports on methods for quantification of TC, LDL-C, and ApoB in DBS. Assay validation results were within acceptable limits for newborn screening. This is an important first step toward the identification of newborns with HeFH.
Orchestration of Immune Cells Contributes to Fibrosis in IgG4-Related Disease
Authors: Kaneko Naoki , Moriyama Masafumi , Maehara Takashi , Chen Hu , Miyahara Yuka , Nakamura Seiji
Published: February 2022

This review summarizes recent progress in understanding the pathogenesis of IgG4-related disease (IgG4-RD), with a focus on fibrosis. Several studies reported that CD4+ T cells with cytotoxic activity promoted by the secretion of granzyme and perforin, cytotoxic CD4+ T cells (CD4+CTLs), and disease-specific activated B cells, infiltrated inflamed tissues and cooperated to induce tissue fibrosis in autoimmune fibrotic diseases such as IgG4-RD, systemic sclerosis, and fibrosing mediastinitis. An accumulation of cells undergoing apoptotic cell death induced by CD4+CTLs and CD8+CTLs followed by macrophage-mediated clearing and finally tissue remodeling driven by cytokines released by CD4+CTLs, activated B cells, and M2 macrophages may contribute to the activation of fibroblasts and collagen production. In IgG4-RD, this process likely involves the apoptosis of non-immune, non-endothelial cells of mesenchymal origin and subsequent tissue remodeling. In summary, CD4+CTLs infiltrate affected tissues where they may cooperate with activated B cells, CD8+CTLs, and M2 macrophages, to induce apoptosis by secreting cytotoxic cytokines. These immune cells also drive fibrosis by secreting pro-fibrotic molecules in IgG4-RD.
Resin-Rich Volumes (RRV) and the Performance of Fibre-Reinforced Composites: A Review
Authors: Mahmood Amjed Saleh , Summerscales John , James Malcolm Neil
Published: February 2022

This review considers the influence of resin-rich volumes (RRV) on the static and dynamic mechanical and physical behaviour of fibre-reinforced composites. The formation, shape and size, and measurement of RRV in composites, depending upon different fabric architectures and manufacturing processes, is discussed. The majority of studies show a negative effect of RRV on the mechanical behaviour of composite materials. The main factors that cause RRV are (a) the clustering of fibres as bundles in textiles, (b) the stacking sequence, (c) the consolidation characteristics of the reinforcement, (d) the resin flow characteristics as a function of temperature, and (e) the composite manufacturing process and cure cycle. RRV are stress concentrations that lead to a disproportionate decrease in composite strength. Those who are considering moving from autoclave consolidation to out-of-autoclave (OOA) processes should be cautious of the potential effects of this change.
A Mathematical Modeling Method for an Analytical Solution of Ship Hydrodynamic Pressure Fields in Complex Restricted Waters
Authors: Deng Hui , Zhang Zhihong , Yi Wenbin , Xia Weixue
Published: February 2022

A ship advancing in restricted waters may cause a change in the surrounding velocity field, which in turn results in the hydrodynamic pressure field according to the variations in the ship speed. Accurate prediction of ship hydrodynamic pressure fields in restricted waters is therefore essential and important in the military and engineering fields. Based on the potential flow theory and the thin-ship assumption, dividing complex restricted waters with varying depths into the inner and outer domains with constant depths, a mathematical modeling method is developed and carried out for analyzing and solving the partial differential equations consisting of the governing equation with a dispersion effect, initial and boundary conditions, then the analytical solution of hydrodynamic pressure field caused by a ship advancing in complex restricted waters is obtained. The continuity of the analytical solution is confirmed and the correctness of the analytical solution is validated by simplifying to a simple water and comparing with available data. Moreover, the mathematical modeling method can be extended to study the hydrodynamic problems of ships in more complex waters.
Implantable Devices for the Treatment of Breast Cancer
Authors: Pial Mohammad Mohtasim Hamid , Tomitaka Asahi , Pala Nezih , Roy Upal
Published: February 2022

Breast cancer is one of the leading causes of death in the female population worldwide. Standard treatments such as chemotherapy show noticeable results. However, along with killing cancer cells, it causes systemic toxicity and apoptosis of the nearby healthy cells, therefore patients must endure side effects during the treatment process. Implantable drug delivery devices that enhance therapeutic efficacy by allowing localized therapy with programmed or controlled drug release can overcome the shortcomings of conventional treatments. An implantable device can be composed of biopolymer materials, nanocomposite materials, or a combination of both. This review summarizes the recent research and current state-of-the art in these types of implantable devices and gives perspective for future directions.
The Continuing Emergence of Candida blankii as a Pathogenic Fungus: A New Case of Fungemia in a Patient Infected with SARS-CoV-2
Authors: Mirchin Ryan , Czeresnia Jonathan M. , Orner Erika P. , Chaturvedi Sudha , Murphy Kerry , Nosanchuk Joshua D.
Published: February 2022

Candida blankii is a recently recognized human pathogen, with most cases of the infection being reported in the immunocompromised. We here describe the case of a critically ill elderly woman with COVID-19 who developed a C. blankii bloodstream infection from a femoral central venous catheter. Aspergillus niger was also isolated from her respiratory secretions. The patient was started on voriconazole for empiric coverage of both A. niger, and at that time, unidentified yeast was found in the blood. Fevers persisted, and the patient expired six days after the yeast was first isolated. Almost one month after her death, C. blankii was identified as the cause of fungemia by sequencing of the internal transcribed spacer (ITS) region of the ribosomal gene and BLAST searching against two databases (performed by a reference laboratory). The isolate demonstrated high minimum inhibitory concentrations (MICs) to azoles and low MICs to amphotericin B, similar to previously described isolates. Timely identification of C. blankii would have prompted different empiric antifungal choices and possibly changed the final outcome. Clinicians should be aware of the pathological potential of C. blankii, the challenges of correctly identifying the organism, and its susceptibility patterns to common antifungals. There is an urgent need to improve assays for C. blankii identification, which will aid in accurate and timely pathogen identification, and appropriate therapeutic management.
Haplotype-Resolved Genome Analyses Reveal Genetically Distinct Nuclei within a Commercial Cultivar of Lentinula edodes
Authors: Gao Qi , Yan Dong , Song Shuang , Fan Yangyang , Wang Shouxian , Liu Yu , Huang Yu , Rong Chengbo , Guo Yuan , Zhao Shuang , Qin Wentao , Xu Jianping
Published: February 2022

Lentinula edodes is a tetrapolar basidiomycete with two haploid nuclei in each cell during most of their life cycle. Understanding the two haploid nuclei genome structures and their interactions on growth and fruiting body development has significant practical implications, especially for commercial cultivars. In this study, we isolated and assembled the two haploid genomes from a commercial strain of L. edodes using Illumina, HiFi, and Hi-C technologies. The total genome lengths were 50.93 Mb and 49.80 Mb for the two monokaryons SP3 and SP30, respectively, with each assembled into 10 chromosomes with 99.63% and 98.91% anchoring rates, respectively, for contigs more than 100 Kb. Genome comparisons suggest that two haploid nuclei likely derived from distinct genetic ancestries, with ~30% of their genomes being unique or non-syntenic. Consistent with a tetrapolar mating system, the two mating-type loci A (matA) and B (matB) of L. edodes were found located on two different chromosomes. However, we identified a new but incomplete homeodomain (HD) sublocus at ~2.8 Mb from matA in both monokaryons. Our study provides a solid foundation for investigating the relationships among cultivars and between cultivars and wild strains and for studying how two genetically divergent nuclei coordinate to regulate fruiting body formation in L. edodes.
Shared Context Facilitates Lexical Variation in Sign Language Emergence
Authors: Mudd Katie , de Vos Connie , de Boer Bart
Published: February 2022

It has been suggested that social structure affects the degree of lexical variation in sign language emergence. Evidence from signing communities supports this, with smaller, more insular communities typically displaying a higher degree of lexical variation compared to larger, more dispersed and diverse communities. Though several factors have been proposed to affect the degree of variation, here we focus on how shared context, facilitating the use of iconic signs, facilitates the retention of lexical variation in language emergence. As interlocutors with the same background have similar salient features for real world concepts, shared context allows for the successful communication of iconic mappings between form and culturally salient features (i.e., the meaning specific to an individual based on their cultural context). Because in this case the culturally salient features can be retrieved from the form, there is less pressure to converge on a single form for a concept. We operationalize the relationship between lexical variation and iconic affordances using an agent-based model, studying how shared context and also population size affects the degree of lexical variation in a population of agents. Our model provides support for the relationship between shared context, population size and lexical variation, though several extensions would help improve the explanatory power of this model.
Homologous Use of Allogeneic Umbilical Cord Tissue to Reduce Knee Pain and Improve Knee Function
Authors: Timmons Ruben Berrocal , Sugaya Kiminobu , Bane Lori Deneke
Published: February 2022

To determine if knee pain subjects who received cryopreserved umbilical cord tissue (UCT) injected into knee joints experience less knee pain, better function, decreased physical limitations, and reduction of medications (opiates, NSAIDs, and acetaminophen) over a 24 week period, Visual Analog Scale (VAS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and medication usage data were recorded for 30 consenting human knee pain subjects receiving UCT at a single site in the United States. Subject profile information was gathered and analyzed to gain insight into the effects of age, sex, and BMI on improvement over time. Mean resting VAS scores and mean VAS scores with activity improved over 24 weeks (from 1.95 to 0.83 and from 6.28 to 2.87, respectively, p < 0.001). There was no strong evidence of a correlation between sex and VAS scores. There were statistically significant correlations for BMI vs. pre-injection VAS with activity scores and Age vs. pre-injection VAS with activity scores (r = 0.402, p = 0.028 and r = 0.434, p = 0.017, respectively). Mean WOMAC scores improved from 44.7 to 18.5 over 24 weeks (p < 0.001). 77.8% of patients who used medications at the beginning of the study reduced or eliminated medication use. The analysis demonstrates that injections with UCT decrease pain, improve physical function, and allow for less medication use for at least 24 weeks.
Arbitrarily Accurate Analytical Approximations for the Error Function
Authors: Howard Roy M.
Published: February 2022

A spline-based integral approximation is utilized to define a sequence of approximations to the error function that converge at a significantly faster manner than the default Taylor series. The real case is considered and the approximations can be improved by utilizing the approximation erf(x)≈1 for |x|>xo and with xo optimally chosen. Two generalizations are possible; the first is based on demarcating the integration interval into m equally spaced subintervals. The second, is based on utilizing a larger fixed subinterval, with a known integral, and a smaller subinterval whose integral is to be approximated. Both generalizations lead to significantly improved accuracy. Furthermore, the initial approximations, and those arising from the first generalization, can be utilized as inputs to a custom dynamic system to establish approximations with better convergence properties. Indicative results include those of a fourth-order approximation, based on four subintervals, which leads to a relative error bound of 1.43 × 10−7 over the interval [0, ∞]. The corresponding sixteenth-order approximation achieves a relative error bound of 2.01 × 10−19. Various approximations that achieve the set relative error bounds of 10−4, 10−6, 10−10, and 10−16, over [0, ∞], are specified. Applications include, first, the definition of functions that are upper and lower bounds, of arbitrary accuracy, for the error function. Second, new series for the error function. Third, new sequences of approximations for exp(−x2) that have significantly higher convergence properties than a Taylor series approximation. Fourth, the definition of a complementary demarcation function eC(x) that satisfies the constraint eC2(x)+erf2(x)=1. Fifth, arbitrarily accurate approximations for the power and harmonic distortion for a sinusoidal signal subject to an error function nonlinearity. Sixth, approximate expressions for the linear filtering of a step signal that is modeled by the error function.
Clinical Significance of the Neutrophil–Lymphocyte Ratio as an Early Predictive Marker for Adverse Outcomes in Patients with Acute Cholangitis
Authors: Lee Sang-Hoon , Lee Tae-Yoon , Jeong Jong-Hyeon , Cheon Young-Koog
Published: February 2022

Background and objectives: Acute cholangitis can be life-threatening if not recognized early. We investigated the predictive value of the neutrophil–lymphocyte ratio (NLR) in acute cholangitis. Materials and Methods: We retrospectively evaluated 206 patients with acute cholangitis who underwent biliary drainage. The severity of acute cholangitis was graded according to the Tokyo 2018 guideline. Patients were dichotomized according to the acute cholangitis severity (mild/moderate vs. severe), the presence of shock requiring a vasopressor/inotrope, and blood culture positivity. The baseline NLR, white blood cell (WBC) count, and C-reactive protein (CRP) levels were compared between groups. Results: The severity of acute cholangitis was graded as mild, moderate, or severe in 71 (34.5%), 107 (51.9%), and 28 (13.6%) patients, respectively. Ten patients (4.8%) developed shock. Positive blood culture (n = 50) was observed more frequently in severe acute cholangitis (67.9% vs. 17.4%, p < 0.001). The NLR was significantly higher in patients with severe cholangitis, shock, and positive blood culture. The area under the curve (AUC) for the NLR, WBC, and CRP for severe acute cholangitis was 0.87, 0.73, and 0.74, respectively. The AUC for the NLR, WBC, and CRP for shock was 0.81, 0.64, and 0.67, respectively. The AUC for the NLR, WBC, and CRP for positive blood culture was 0.76, 0.64, and 0.61, respectively; the NLR had greater power to predict disease severity, shock, and positive blood culture. The optimal cut-off value of the baseline NLR for the prediction of severe acute cholangitis, shock, and positive blood culture was 15.24 (sensitivity, 85%; specificity, 79%), 15.54 (sensitivity, 80%; specificity, 73%), and 12.35 (sensitivity, 72%; specificity, 70%), respectively. The sequential NLR values from admission to 2 days after admission were significantly higher in patients with severe cholangitis and shock. Conclusions: An elevated NLR correlates with severe acute cholangitis, shock, and positive blood culture. Serial NLR can track the clinical course of acute cholangitis.
Evaluation of the Effectiveness of Dry Needling in the Treatment of Myogenous Temporomandibular Joint Disorders
Authors: Dib-Zakkour Juan , Flores-Fraile Javier , Montero-Martin Javier , Dib-Zakkour Sara , Dib-Zaitun Ibrahim
Published: February 2022

Background and Objectives: The objective of our clinical trial was to determine the effectiveness of the deep dry needling technique (DDN) (neuromuscular deprogramming) as a first step in the treatment of temporomandibular disorders. Methods and Materials: The double-blind randomized clinical trial comprised 36 patients meeting the inclusion criteria who had signed the corresponding informed consent form. The participants were randomly distributed into two groups, the Experimental group (Group E) and the Control group (Group C). Group E received bilateral DDN on the masseter muscle, while Group C received a simulation of the technique (PN). All the participants were evaluated three times: pre-needling, 10 min post-needling, and through a follow-up evaluation after 15 days. These evaluations included, among other tests: pain evaluation using the Visual Analog Scale (VAS) and bilateral muscle palpation with a pressure algometer; evaluation of the opening pattern and range of the mouth, articular sounds and dental occlusion using T-scans; and electromyography, which was used to evaluate the muscle tone of the masseter muscles, in order to control changes in mandibular position. Results: Digital control of occlusion using Tec-Scan (digital occlusion analysis) showed a significant reduction both in the time of posterior disclusion and in the time needed to reach maximum force in an MI position after needling the muscle, which demonstrated that there were variations in the static position and the trajectory of the jaw. The symmetry of the arch while opening and closing the mouth was recovered in a centric relation, with an increase in the opening range of the mouth after the procedure. Conclusions: facial pain is significantly reduced and is accompanied by a notable reduction in muscle activity after needling its trigger points.
Non-Marked Hypoechogenic Nodules: Multicenter Study on the Thyroid Malignancy Risk Stratification and Accuracy Based on TIRADS Systems Comparison
Authors: Prieditis Peteris , Radzina Maija , Mikijanska Madara , Liepa Mara , Stepanovs Kaspars , Grani Giorgio , Durante Cosimo , Lamartina Livia , Trimboli Pierpaolo , Cantisani Vito
Published: February 2022

Background and Objectives: The aim of the study was to evaluate the predictive value of the ultrasound criterion “non-marked hypoechogenicity” for malignancy and to determine whether classification of these nodules as TIRADS 3 could improve the overall accuracy of consequently adjusted M-TIRADS score. Materials and Methods: A total of 767 patients with 795 thyroid nodules were subject to ultrasonography examination and ultrasound-guided fine needle aspiration biopsy. Nodules were classified by Kwak TIRADS and modified (M-TIRADS) categories 4A, 4B, and 5 according to number of suspicious US features (marked hypoechogenicity, microlobulated or irregular margins, microcalcifications, taller-than-wide shape, metastatic lymph nodes). Non-marked hypoechoic nodules were classified as TIRADS 3. Results: Thyroid nodules were classified as TIRADS 2, 3, 4A, 4B, and 5 in 14.5, 57.5, 14.2, 8.1, and 5.7%, respectively. Only histopathologic results (125 nodules underwent surgery) and highly specific cytology results (Bethesda II, VI) were accepted as a standard of reference, forming a sub-cohort of 562/795 nodules (70.7%). Malignancy was found in 7.7%. Overall, M-TIRADS showed sensitivity/specificity of 93.02/81.31%, and for PPV/NPV, these were 29.2/99.29%, respectively (OR—18.62). Irregular margins showed the highest sensitivity and specificity (75.68/93.74%, respectively). In TIRADS 3 category, 37.2% nodules were isoechoic, 6.6% hyperechoic, and 52.2% hypoechoic (there was no difference of malignancy risk in hypoechoic nodules between M-TIRADS and Kwak systems—0.9 vs. 0.8, respectively). Accuracy of M-TIRADS classification in this cohort was 78.26% vs. 48.11% for Kwak. Conclusions: The non-marked hypoechoic nodule pattern correlated with low risk of malignancy; classification of these nodules as TIRADS 3 significantly improved the predictive value and overall accuracy of the proposed M-TIRADS scoring with malignancy risk increase in TIRADS 4 categories by 20%; and no significant alteration of malignancy risk in TIRADS 3 could contribute to reducing overdiagnosis, obviating the need for FNA.