bluuebunny/crossref_metadata_2025 huggingface dataset:
Biogenic Synthesis of Silver Nanoparticles Mediated by Aronia melanocarpa and Their Biological Evaluation
Authors: Corciovă Andreia , Mircea Cornelia , Fifere Adrian , Turin-Moleavin Ioana-Andreea , Roşca Irina , Macovei Irina , Ivănescu Bianca , Vlase Ana-Maria , Hăncianu Monica , Burlec Ana Flavia
Published: September 2024

In the present study, two A. melanocarpa berry extracts were used for the synthesis of silver nanoparticles (AgNPs). After the optimization of synthesis, the AgNPs were characterized using UV–Vis, FTIR, EDX, DLS, and STEM analyses. The stability in different media, phytotoxicity, as well as antimicrobial and antioxidant activities were also evaluated. The ideal synthesis conditions were represented by a 3 mM AgNO3 concentration, 1:9 extract:AgNO3 volume ratio, alkaline medium, and stirring at 40 °C for 120 min. The synthesis was confirmed by the surface plasmon resonance (SPR) peak at 403 nm, and the strong signal at 3 keV from the EDX spectra. FTIR analysis indicated that polyphenols, polysaccharides, and amino acids could be the compounds responsible for synthesis. Stability tests and the negative zeta potential values showed that phytocompounds also play a role in the stabilization and capping of AgNPs. The preliminary phytotoxicity studies on T. aestivum showed that both the extracts and their corresponding AgNPs had an impact on the growth of roots and shoots as well as on the microscopic structure of leaves. The synthesized AgNPs presented antimicrobial activity against S. aureus, E. coli, and C. albicans. Moreover, considering the results obtained in the lipoxygenase inhibition, the DPPH and hydroxyl scavenging activities, and the ferrous ion chelating assay, AgNPs exhibit promising antioxidant activity.
Novel Diagnosis and Therapeutics Approaches in Retina Diseases
Authors: Mete Maurizio , Iacovello Daniela , Maggio Emilia
Published: September 2024

We have warmly welcomed the opportunity to propose the Special Issue titled “Novel Diagnosis and Therapeutics Approaches in Retina Diseases”, and numerous authors have decided to submit their works to it [...]
Recent Advancements in Fabrication of Metal Matrix Composites: A Systematic Review
Authors: Sarmah Pallab , Gupta Kapil
Published: September 2024

Metal matrix composites (MMCs) are essential materials in various industries due to superior properties, such as high strength-to-weight ratios, better corrosion resistance, improved wear resistance and adaptability, developed by continuous improvements in their fabrication methods. This helps to meet the growing demand for high-performance and sustainable products. The industries that stand to gain the most are automotive and aerospace, where MMCs are used for car parts, airplane frames, and jet engines that need to be strong and lightweight. Furthermore, MMCs are being extensively used in the biomedical industry for implants and medical equipment because of their suitable mechanical integrity and corrosion resistance. Applications in heavy construction, defense, and even space exploration are noteworthy. The advancements in fabrication of MMCs have revolutionized the composite industry with their improved mechanical, tribological, and metallurgical properties. This review article offers an introduction and thorough examination of the most recent advancements (mostly within the last five years) in fabrication methods of MMCs. The novelty and modernization in the traditional processes and advanced processes are covered, along with discussing the process parameters’ effects on the microstructure and properties of the composites. The review focuses on features and prospective applications of MMCs that have been greatly improved and extended due to such advancements. The most recent methods for developing MMCs, including friction stir processing (FSP), ultrasonic-assisted stir casting, and additive manufacturing, are discussed. Artificial intelligence and machine learning interventions for composite manufacturing are also included in this review. This article aims to assist researchers and scholars and encourage them to conduct future research and pursue innovations to establish the field further.
Thermal Annealing Effect on Elastoplastic Behaviour of Al/Cu Bimetal during Three-Point Bending
Authors: Uścinowicz Robert
Published: September 2024

This paper presents the results of experimental studies on the effects of temperature and time of annealing on the elastoplastic properties of bimetallic aluminium–copper sheets. Mechanical tests were carried out on flat samples previously heated to temperatures of 250, 350, 450, and 500 °C for 40, 90, and 150 min. At the beginning of the tests, the elastic constants and internal friction energy were determined after thermal exposure using the impulse vibration exposure method. Further tests were carried out on the same samples using the three-point bending test. Based on the tests, the following quantities were determined and analysed: elasticity angles, translocations of the neutral axes of the cross-sections of samples, and changes in the values of bending moments plasticizing the extreme layers of bimetallic Al/Cu samples resulting from thermal interactions. The final part of this paper presents the results of measurements of the thickness of diffusion zones at the interface and their effect on the stability of the joint after annealing. The studies that were conducted indicate the dominant influence of the thermal factor on the properties of the Al/Cu bimetal above the temperature of 350 °C, which leads to the weakening of its strength and the degradation of the structure at the metallic phase boundary.
Thomson/Joule Power Compensation and the Measurement of the Thomson Coefficient
Authors: Garrido Javier , Manzanares José A.
Published: September 2024

The energy transported by the electric current that circulates a thermoelectric element (TE) varies with position due to the Joule and Thomson effects. The Thomson effect may enhance or compensate the Joule effect. A method for measuring the Thomson coefficient of a TE is presented. This method is based on the total compensation of the Joule and Thomson effects. The electric current then flows without delivering power to the TE or absorbing power from it. For a TE, the global Thomson/Joule compensation ratio Φ¯T/J is defined as the ratio of the power absorbed by the current due to the Thomson effect and the power delivered by the current to the TE due to the Joule effect. It can be expressed as Φ¯T/J=I0/I, where I is the electric current and I0 is the zero-power current, a quantity that is proportional to the average Thomson coefficient. When I=I0, the Thomson effect exactly compensates the Joule effect and the net power delivered by the current to the TE is zero. Since the power delivered by the current is related to the temperature distribution, temperature measurements for currents around I0 can be used as the basis for a measurement technique of the Thomson coefficient. With varying current, the difference between the temperature at the center of the TE and the mean temperature between its extremes reverses its sign at the zero-power current, I=I0. This observation suggests the possibility of measuring the Thomson coefficient, but a quantitative analysis is needed. With calculations using the constant transport coefficients model for Bi2Te0.94Se0.063  and Bi0.25Sb0.752Te3, it is theoretically shown that a null temperature detector with a sensitivity of the order of 1 mK allows for the accurate determination of the Thomson coefficient.
Experimental and Numerical Study on Lightweight-Foamed-Concrete-Filled Widened Embankment of High-Speed Railway
Authors: Hao Didi , Miao Changqing , Fang Shisheng , Wang Xudong , Shu Qiaoqiao
Published: September 2024

To study the performance of lightweight foamed concrete (LWFC) in widened embankments of high-speed railways, this study first conducted numerous strength, permeability, and water immersion tests to investigate the mechanical properties and water resistance of LWFC with designed dry densities of 550, 600, and 650 kg/m3. Secondly, a field test was performed to analyze the behavior of the deformation and the internal pressure within the LWFC-filled portions. Furthermore, a parametric study via numerical modeling was performed to investigate the effects of four key factors on the performance of the LWFC-filled, widened embankments. Results showed that LWFC possesses adequate bearing capacity and impermeability to meet high-speed railway embankment widening requirements. However, water seepage reduces LWFC strength. The additional pressure from LWFC filling increases initially but then decreases once dehydration occurs. The settlement induced by LWFC accounted for 71% of the total filling height, which is only 37.5% of the total settlement after construction. The parametric study results show that the maximum settlement of widened and existing portions induced by LWFC was 46.3–49.6% and 48.3–53.2% of those induced by traditional fillers due to the LWFC’s lower density as well as their better self-supporting ability. Making an appropriate reduction in the thickness of the retain wall installed against the LWFC-filled widened embankment of the high-speed railway generates a few variations in the lateral deformation of the wall. Furthermore, the effects of the pile offset on the deformation of the LWFC-filled embankment were more sensitive compared to the diameter of the piles.
Effect of Silane-Containing Adhesives on Repair Bond Strength between Fresh and Aged Composite Materials—A Pilot Study
Authors: Gajski Petra , Par Matej , Tarle Zrinka , Marovic Danijela
Published: September 2024

This study investigated the effects of different surface treatments and a silane-containing adhesive on the repair bond strength between fresh and aged resin composites. A total of 140 composite specimens were prepared and aged for 24 h or 4 months. Each group was subdivided into seven subgroups (n = 10) depending on the surface treatment (no surface treatment (NT), sandblasting (SAND), or Sof-lex coarse disc (DISC)) in combination with the use of the silane-containing adhesive ScotchBond Universal Plus (SBU) or an adhesive without silane Prime&Bond Universal (P&B). The same composite was used for the repair as for the primary specimen. Specimens were dark stored in distilled water at 37 °C for 28 days. Shear bond strength was tested at a crosshead speed of 0.5 mm/min. The Kruskal–Wallis test with Bonferroni’s post-hoc adjustment (α = 0.05) and the Mann–Whitney U-test were used for the statistical analysis. The results are shown as the median with the interquartile range. The highest bond strength (MPa) was achieved after 24 h in the DISC+P&B (20.39(16.85–28.83)). In the fresh 24 h group, the SAND+P&B (12.25(8.28–15.05)) and DISC+SBU (18.37(15.16–21.29)) were statistically similar. In the 4-month groups, both adhesives and surface treatments performed similarly. The NT, SAND, and DISC groups without adhesives had the lowest bond strength. In the repair of fresh or aged composite, the silane-containing adhesive SBU was not superior to the adhesive without the silane (P&B).
Developing a Cobalt Phosphide Catalyst with Combined Cobalt Defects and Phosphorus Vacancies to Boost Oxygen Evolution Reaction
Authors: Ou Weihua , Li Ligui , Zhou Wei , Chen Minzhe , Zhu Chuheng , Zhu Xiaoyan , Yuan Ke
Published: September 2024

Defect engineering, by adjusting the surface charge and active sites of CoP catalysts, significantly enhances the efficiency of the oxygen evolution reaction (OER). We have developed a new Co1−xPv catalyst that has both cobalt defects and phosphorus vacancies, demonstrating excellent OER performance. Under both basic and acidic media, the catalyst incurs a modest overvoltage, with 238 mV and 249 mV needed, respectively, to attain a current density of 10 mA cm−2. In the practical test of alkaline electrocatalytic water splitting (EWS), the Co1−xPv || Pt/C EWS shows a low cell voltage of 1.51 V and superior performance compared to the noble metal-based EWS (RuO2 || Pt/C, 1.66 V). This catalyst’s exceptional catalytic efficiency and longevity are mainly attributed to its tunable electronic structure. The presence of cobalt defects facilitates the transformation of Co2+ to Co3+, while phosphorus vacancies enhance the interaction with oxygen species (*OH, *O, *OOH), working in concert to improve the OER efficiency. This strategy offers a new approach to designing transition metal phosphide catalysts with coexisting metal defects and phosphorus vacancies, which is crucial for improving energy conversion efficiency and catalyst performance.
Mechanism of Fatigue-Life Extension Due to Dynamic Strain Aging in Low-Carbon Steel at High Temperature
Authors: Fang Zheng , Wang Lu , Yu Fengyun , He Ying , Wang Zheng
Published: September 2024

An enhancement in fatigue life for ferrite–pearlite low-carbon steel (LCS) at high temperature (HT) has been discovered, where it increased from 190,873 cycles at room temperature (RT) to 10,000,000 cycles at 400 °C under the same stress conditions. To understand the mechanism behind this phenomenon, the evolution of microstructure and dislocation density during fatigue tests was comprehensively investigated. High-power X-ray diffraction (XRD) was employed to analyze the evolution of total dislocation density, while Electron Backscatter Diffraction (EBSD) and High-Resolution EBSD (HR-EBSD) were conducted to reveal the evolutions of kernel average misorientation (KAM), geometrically necessary dislocations (GND) and elastic strains. Results indicate that the enhancement was attributed to the dynamic strain aging (DSA) effect above the upper temperature limit, where serration and jerky flow disappeared but hindrance of dislocations persisted. Due to the DSA effect, periods of increase and decrease in the total dislocations were observed during HT fatigue tests, and the fraction of screw dislocations increased continuously, caused by viscous movement of the screw dislocations. Furthermore, the increased fraction of screw dislocations resulted in a lower energy configuration, reducing slip traces on sample surfaces and preventing fatigue-crack initiation.
Use of Milled Acanthocardia tuberculate Seashell as Fine Aggregate in Self-Compacting Mortars
Authors: González-Caro Ágata , Merino-Lechuga Antonio Manuel , Fernández-Ledesma Enrique , Fernández-Rodríguez José María , Jiménez José Ramón , Suescum-Morales David
Published: September 2024

This study focuses on the feasibility of using ground Acanthocardia tuberculate seashells as fine aggregates for self-compacting mortar production. The obtained results show a promising future for coastal industries as their use eliminates waste products and improves the durability of these materials. The use of Acanthocardia tuberculate recycled aggregate, in terms of durability, improves the performance of all mixes made with seashells compared to those made with natural sand, although it decreases workability and slightly reduces mechanical strength. Proper mix design has beneficial effects, as it improves compressive strength, especially when the powder/sand ratio is 0.7. Three replacement ratios based on the volume (0%, 50%, and 100%) of natural limestone sand with recycled fine aggregate from Acanthocardia tuberculate seashells, and three different dosages modifying the powder/sand ratio (0.6, 0.7, and 0.8), were tested. The fresh-state properties of each self-compacting mixture were evaluated based on workability. The mineralogical phases of the hardened mixtures were characterised using X-ray diffraction, thermogravimetry, and differential analyses. Subsequently, the mechanical and durability properties were evaluated based on the compressive and flexural strengths, dry bulk density, accessible porosity for water and water absorption, drying shrinkage, mercury intrusion porosimetry, and water absorption by capillarity. Therefore, the use of Acanthocardia tuberculate seashells in cement-based systems contributes to circular economy.
Characterization of Thermal Expansion Coefficient of 3D Printing Polymeric Materials Using Fiber Bragg Grating Sensors
Authors: Matsika-Klossa Constantina , Chatzidai Nikoleta , Kousiatza Charoula , Karalekas Dimitrios
Published: September 2024

This work aims at the determination of the coefficient of thermal expansion (CTE) of parts manufactured through the Fused Deposition Modeling process, employing fiber Bragg grating (FBG) sensors. Pure thermoplastic and composite specimens were built using different commercially available filament materials, including acrylonitrile butadiene styrene, polylactic acid, polyamide, polyether-block-amide (PEBA) and chopped carbon fiber-reinforced polyamide (CF-PA) composite. During the building process, the FBGs were embedded into the middle-plane of the test specimens, featuring 0° and 90° raster printing orientations. The samples were then subjected to thermal loading for measuring the thermally induced strains as a function of applied temperature and, consequently, the test samples’ CTE and glass transition temperature (Tg) based on the recorded FBG wavelengths. Additionally, the integrated FBGs were used for the characterization of the residual strain magnitudes both at the end of the 3D printing process and at the end of each of the two consecutively applied thermal cycles. The results indicate that, among all tested materials, the CF-PA/0° specimens exhibited the lowest CTE value of 14 × 10−6/°C. The PEBA material was proven to have the most isotropic thermal response for both examined raster orientations, 0° and 90°, with CTE values of 117 × 10−6/°C and 108 × 10−6/°C, respectively, while similar residual strains were also calculated in both printing orientations. It is presented that the followed FBG-based methodology is proven to be an excellent alternative experimental technique for the CTE characterization of materials used in 3D printing.
Study on the Flexural Performance of Ultrahigh-Performance Concrete–Normal Concrete Composite Slabs
Authors: Sun Zizhou , Li Xianjing , Liu Chao
Published: September 2024

In recent years, there have been an increasing number of examples of using ultrahigh-performance concrete (UHPC) as a pavement layer to form an ultrahigh-performance concrete–normal concrete (UHPC–NC) composite structure to improve the bearing capacity of bridges. In order to study the flexural performance of this kind of structure, this research studied the flexural performance of UHPC–NC composite slabs, with UHPC in the compression zone, using experiments, numerical simulation, and theoretical analysis. The results showed the following. Firstly, after the UHPC–NC interface had been chiseled, there was no obvious slip between the two materials during the test, and the composite plate was always subjected to synergistic stress. Secondly, the composite slabs in the compression zone of the UHPC were all subjected to bending failure, and the cooperative working performance of each part under the bending load was good, indicating that the composite slab had a unique failure mode and a high bearing capacity. Thirdly, increasing the thickness of the UHPC significantly improved the flexural capacity of the composite plate, and the maximum increase was about 15%. Increasing the reinforcement ratio of the tensile steel rebars also had an increasing effect, with a maximum increase of about 181%. Finally, the proposed formula for calculating the flexural capacity of composite slabs with UHPC in the compression zone could accurately predict the bearing capacity of said slabs. The calculated results were in good agreement with the experimental values, and the error was small.
A Multi-Layer Multi-Pass Weld Bead Cross-Section Morphology Extraction Method Based on Row–Column Grayscale Segmentation
Authors: Lei Ting , Gong Shixiang , Wu Chaoqun
Published: September 2024

In the field of welding detection, weld bead cross-section morphology serves as a crucial indicator for analyzing welding quality. However, the extraction of weld bead cross-section morphology often relies on manual extraction based on human expertise, which can be limited in consistency and operational efficiency. To address this issue, this paper proposes a multi-layer multi-pass weld bead cross-section morphology extraction method based on row–column grayscale segmentation. The weld bead cross-section morphology image is pre-processed and then segmented into rows and columns based on the average gray value of the image. In order to extract the feature of multi-layer multi-pass weld feature images, an outline showing the binarization threshold is selected for each segmented image (ESI). Then, the weld contour of ESI is extracted before image fusion and morphological processing. Finally, the weld feature parameters (circumference, area, etc.) are extracted from the obtained weld feature image. The results indicate that the relative errors in circumference and area are within 10%, while the discrepancies in maximum weld seam width and maximum weld seam height can be close to the true value. The quality assessment falls within a reasonable range, the average value of SSIM is above 0.9 and the average value of PSNR is above 60 on average. The results demonstrate that this method is feasible for extracting the general contour features of multi-layer multi-pass weld bead cross-section morphology images, providing a basis for further detailed analysis and improvement in welding quality assessment.
Frost Resistance and Microscopic Properties of Recycled Coarse Aggregate Concrete Containing Chemical Admixtures
Authors: Song Yongyuan , Zhou Wenjuan , Zhang Chen , Yang Can
Published: September 2024

In order to increase the suitability of coarse recycled concrete aggregates and improve the frost resistance of recycled coarse aggregate concrete, this study aims to investigate the effects of an antifreeze-type water-reducing admixture, air-entraining admixture, and antifreeze admixture on the frost resistance of recycled coarse aggregate concrete. The effectiveness of these admixtures is gauged by the mass loss rate and the relative dynamic modulus of elasticity (RDM). Mercury-impressed porosimetry (MIP), super depth of field microscopy, and scanning electron microscopy (SEM) were employed to characterize the hydration products, microstructure, and pore structure of recycled coarse aggregate concrete, with a view to establishing a connection between the microstructural characteristics and the macro properties and analyzing the micro-mechanism of the improvement effect of frost resistance. The test results demonstrate that the admixtures have a significant impact on the frost resistance of recycled coarse aggregate concrete. In particular, the recycled coarse aggregate concrete with an antifreeze admixture (dosage of 1%) and a water–cement ratio of 0.41 exhibited a mass loss of only 1.23% after 200 freezing and thawing cycles, a relative dynamic modulus of elasticity of up to 93.97%; however, the control group had reached the stopping condition at 150 freeze–thaw cycles with more than 10% mass loss. The recycled coarse aggregate concrete with added antifreeze admixture had a tight connection between the aggregate and the paste and a more pronounced improvement in the pore structure, indicating excellent resistance to frost damage.
Single-Particle Crushing Test of Coated Calcareous Sand Based on MICP
Authors: Zhu Shuyue , Gong Linxian , Hu Zhazha , Xu Yan , He Yuanyuan , Long Yunyi
Published: September 2024

Calcareous sand is a crucial construction material for island and reef development and reinforcing it using Microbially Induced Calcite Precipitation (MICP) technology is a promising new method. This study employed 3D scanning technology to assess changes in the particle size and morphology of MICP-treated, coated calcareous sand particles. Single-particle crushing tests were conducted to analyze their crushing strength, crushing energy, crushing modes, and fragment fractal dimensions. The results indicated that MICP treatment significantly increased particle size, surface area, and volume, while reducing flatness. At a cementation solution concentration of 1 mol/L, both crushing strength and crushing energy were optimized. The coated particles exhibited three crushing modes: explosive crushing, mixed crushing, and splitting crushing. Thicker coatings led to a tendency for particles to break into larger fragments through the mixed and splitting crushing modes. Fractal analysis revealed that coating thickness directly affects the local crushing characteristics of the particles.
Aquatic Biomaterial Repositories: Comprehensive Guidelines, Recommendations, and Best Practices for Their Development, Establishment, and Sustainable Operation
Authors: Tourapi Christiana , Christoforou Eleni , Gaudêncio Susana P. , Vasquez Marlen I.
Published: September 2024

The alarming pace of species extinction severely threatens terrestrial and aquatic ecosystems, undermining the crucial ecological services vital for environmental sustainability and human well-being. Anthropogenic activities, such as urbanization, agriculture, industrialization, and those inducing climate change, intensify these risks, further imperiling biodiversity. Of particular importance are aquatic organisms, pivotal in biodiscovery and biotechnology. They contribute significantly to natural product chemistry, drug development, and various biotechnological applications. To safeguard these invaluable resources, establishing and maintaining aquatic biomaterial repositories (ABRs) is imperative. This review explores the complex landscape of ABRs, emphasizing the need for standardized procedures from collection to distribution. It identifies key legislative and regulatory frameworks, such as the Nagoya Protocol and EU directives, essential for ensuring responsible and equitable biorepository operations. Drawing on extensive literature and database searches, this study compiles existing recommendations and practices into a cohesive framework with which to guide the establishment and sustainable management of ABRs. Through collaborative efforts and adherence to best practices, ABRs can play a transformative role in the future of marine biotechnology and environmental conservation.
Characterization and Genomic Analyses of dsDNA Vibriophage vB_VpaM_XM1, Representing a New Viral Family
Authors: Wei Zuyun , Li Xuejing , Ai Chunxiang , Dang Hongyue
Published: September 2024

A novel vibriophage vB_VpaM_XM1 (XM1) was described in the present study. Morphological analysis revealed that phage XM1 had Myovirus morphology, with an oblate icosahedral head and a long contractile tail. The genome size of XM1 is 46,056 bp, with a G + C content of 42.51%, encoding 69 open reading frames (ORFs). Moreover, XM1 showed a narrow host range, only lysing Vibrio xuii LMG 21346 (T) JL2919, Vibrio parahaemolyticus 1.1997, and V. parahaemolyticus MCCC 1H00029 among the tested bacteria. One-step growth curves showed that XM1 has a 20-min latent period and a burst size of 398 plaque-forming units (PFU)/cell. In addition, XM1 exhibited broad pH, thermal, and salinity stability, as well as strong lytic activity, even at a multiplicity of infection (MOI) of 0.001. Multiple genome comparisons and phylogenetic analyses showed that phage XM1 is grouped in a clade with three other phages, including Vibrio phages Rostov 7, X29, and phi 2, and is distinct from all known viral families that have ratified by the standard genomic analysis of the International Committee on Taxonomy of Viruses (ICTV). Therefore, the above four phages might represent a new viral family, tentatively named Weiviridae. The broad physiological adaptability of phage XM1 and its high lytic activity and host specificity indicated that this novel phage is a good candidate for being used as a therapeutic bioagent against infections caused by certain V. parahaemolyticus strains.
Effect of Yttrium and Yttria Addition in Self-Passivating WCr SMART Material for First-Wall Application in a Fusion Power Plant
Authors: Chen Jie , Tejado Elena , Rasiński Marcin , Litnovsky Andrey , Nguyen-Manh Duc , Prestat Eric , Whitfield Tamsin , Pastor Jose Ygnacio , Bram Martin , Coenen Jan Willem , Linsmeier Christian , Gonzalez-Julian Jesus
Published: September 2024

The self-passivating yttrium-containing WCr alloy has been developed and researched as a potential plasma-facing armour material for fusion power plants. This study explores the use of yttria (Y2O3) powders instead of yttrium elemental powders in the mechanical alloying process to assess their applicability for this material. Fabricated through field-assisted sintering, WCr-Y2O3 ingots show Y2O3 and Cr-containing oxides (Cr-O and Y-Cr-O) dispersed at grain boundaries (GBs), while WCrY ingots contain Y-O particles at grain boundaries, both resulting from unavoidable oxidation during fabrication. WCr-Y2O3 demonstrates higher flexural strength than WCrY across all temperature ranges, ranging from 850 to 1050 MPa, but lower fracture toughness, between 3 and 4 MPa·√m. Enhanced oxidation resistance is observed in WCr-Y2O3, with lower mass gain as compared to WCrY during the 20-hour oxidation test. This study confirms the effectiveness of both yttria and yttrium in the reactive element effect (REE) for the passivation of WCr alloy, suggesting the potential of Y2O3-doped WCr for first wall applications in a fusion power plant.
Relationship of SOD-1 Activity in Metabolic Syndrome and/or Frailty in Elderly Individuals
Authors: Dzięgielewska-Gęsiak Sylwia , Wysocka Ewa , Fatyga Edyta , Muc-Wierzgoń Małgorzata
Published: September 2024

Introduction: Although aging is a natural phenomenon, in recent years it has accelerated. One key factor implicated in the aging process is oxidative stress. Oxidative stress also plays a role in frailty (frail) and metabolic syndrome (MetS). Methods: A total of 66 elderly persons (65 years old and older) with no acute or severe chronic disorders were assessed for waist circumference (WC), arterial blood pressure, glycemia, glycated hemoglobin (HbA1c), plasma lipids, and activity of erythrocyte superoxide dismutase (SOD-1). Patients were classified as NonMetS-Nonfrail (n = 19), NonMetS-frail (n = 20), MetS-Nonfrail (n = 17), or MetS-frail (n = 10). Results: There were no significant differences in superoxide dismutase activity among investigated elderly groups. However, the data suggest that MetS individuals, both frail and nonfrail, have higher risk factors for cardiovascular disease compared to NonMetS individuals. The correlations analyses of SOD-1 and other metabolic indices suggest that SOD-1 levels may be influenced by age, total cholesterol, HDL cholesterol, and fasting glucose levels in certain groups of seniors. Conclusions: Aging is associated with decreased antioxidant enzyme SOD-1 activity with glucose alteration in frailty syndrome as well as with lipids disturbances in metabolic syndrome. These factors provide a nuanced view of how frailty and metabolic syndrome interact with various health parameters, informing both clinical practice and future research directions.
Variation of MEMS Thin Film Device Parameters under the Influence of Thermal Stresses
Authors: Wen Xiao , Chen Jinchuan , Liu Ruiwen , He Chunhua , Huang Qinwen , Guo Huihui
Published: September 2024

With the advancement of semiconductor manufacturing technology, thin film structures were widely used in MEMS devices. These films played critical roles in providing support, reinforcement, and insulation in MEMS devices. However, due to their microscopic dimensions, the sensitivity of their parameters and performance to thermal stress increased significantly. In this study, a Pirani gauge sample with a multilayer thin film structure was designed and fabricated. Based on this sample, finite element modeling analysis and thermal stress experiments were conducted. The finite element modeling analysis employed a combination of steady-state and transient methods to simulate the deformation and stress distribution of the device at room temperature (25 °C), low temperature (−55 °C), and high temperature (125 °C). The thermal stress test involved placing the sample in a temperature cycling chamber for temperature cycling tests. After the tests, the resonant frequency and surface deformation of the device were measured to quantitatively evaluate the impact of thermal stress on the deformation and resonant frequency parameters of the device. After the experiments, it was found that the clamped-end beams made of Pt were a stress concentration area. Additionally, the repetitive thermal load caused the cantilever beam to move cyclically in the Z direction. This movement altered the deformation of the film and the resonant frequency. The suspended film exhibited concavity, and the overall trend of the resonant frequency was downward. Over time, this could even lead to the fracture of the clamped-end beams. The variation of mechanical parameters derived from finite element simulations and experiments provided an important reference value for device design improvement and played a crucial role in enhancing the reliability of thin film devices.
Patterns of Dietary Supplement Use during the COVID-19 Pandemic in Poland: Focus on Vitamin D and Magnesium
Authors: Grosman-Dziewiszek Patrycja , Jęśkowiak-Kossakowska Izabela , Szeląg Adam , Wiatrak Benita
Published: September 2024

Background: The COVID-19 pandemic has brought significant attention to the role of dietary supplements, particularly Vitamin D, in enhancing immunity and possibly mitigating the severity of the disease. The pandemic has highlighted the importance of nutritional health in preventing severe outcomes from infections. Objective: This study aimed to assess consumption patterns of dietary supplements, with a focus on Vitamin D, among the Polish population during the COVID-19 pandemic and to identify the demographic factors influencing these patterns. Methods: An anonymous survey was conducted in March 2021 among 926 pharmacy patients in Poland. The study analyzed the use of dietary supplements such as vitamin D, magnesium, and others in relation to variables like age, gender, and education level. Statistical analyses were performed using the Pearson chi-square test. Results: The study revealed that 77.1% of the respondents reported using dietary supplements, with Vitamin D being the most frequently mentioned, used by 64.6% of participants. Magnesium was also widely used, with a higher overall prevalence of 67.3%, making it the most commonly consumed supplement. The use of supplements was significantly higher among women and individuals with higher education. Younger age groups, particularly those aged 18–30, were more likely to use supplements. Conclusions: The use of supplements was significantly higher among women, individuals with higher education, and those aged 18–30. However, the findings also indicate a growing awareness and increased use across the general population. This trend reflects increased public awareness of the potential health benefits of these supplements in boosting immunity. However, the study also highlights the need for public education on the risks of over-supplementation and the importance of appropriate dosages.
Prevalence and Characteristics of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 among Salmonella Enteritidis Isolates from Retail Chickens and Children with Gastroenteritis in China
Authors: Liu Liyuan , Yi Shanrong , Xu Xuebin , Zheng Liya , Liu Hong , Zhou Xiujuan
Published: September 2024

A total of 265 Salmonella Enteritidis isolates collected from retail markets and children’s hospitals in Shanghai were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes. Nine of the isolates—7 from the 146 (4.79%) retail chicken-related samples and 2 from the 119 (1.68%) samples from clinical children—were fosfomycin-resistant (FosR). The fosA3 gene was detected in all of the nine FosR isolates, which were located on Inc F-type (8/9, 88.9%) and unknown-type (1/9, 11.1%) transferable plasmids. In total, five plasmid types, namely Inc HI2 (1/9, 11.1%), Inc I1 (3/9, 33.3%), Inc X (8/9, 88.9%), Inc FIIs (9/9, 100%), and Inc FIB (9/9, 100%), were detected in these FosR isolates, which possessed five S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) profiles. The extended-spectrum β-lactamase determinant blaCTX-M-14 subtype was identified in one FosR S. Enteritidis isolate, which was located in a transferable unknown-type plasmid co-carrying fosA3 and tetR genes. Sequence homology analysis showed that this plasmid possessed high sequence similarity to previously reported blaCTX-M-14- and fosA3-positive plasmids from E. coli strains, implying that plasmids carrying the fosA3 gene might be disseminated among Enterobacterales. These findings highlight further challenges in the prevention and treatment of Enterobacteriaceae infections caused by plasmids containing fosA3.
Prospective Analysis of Safety and Efficacy of Tenofovir Alafenamide Fumarate (TAF) in European Real-World Patients with Chronic Hepatitis B: A Single-Centre Real-Word Cohort Study
Authors: Fülöp Balazs , Fischer Janett , Hahn Magdalena , Böhlig Albrecht , Matz-Soja Madlen , Berg Thomas , van Bömmel Florian
Published: September 2024

Background: Tenofovir alafenamide (TAF) is a novel prodrug of tenofovir for the treatment of chronic hepatitis B (CHB) that has shown a favourable renal safety profile while offering suppression of HBV DNA similar to tenofovir disoproxil fumarate (TDF). We aimed to study changes in markers of HBV replication and renal function in a real-world setting in European patients. Methods: In our prospective single-arm, non-interventional observational study, HBeAg-positive and HBeAg-negative patients with chronic HBV mono-infection receiving TAF as their first or following line treatment were enrolled. HBV DNA, HBsAg, markers of bone metabolism, and renal function were determined at baseline and every consecutive 3 months. Results: A total of 50 patients (70% male) were included. The mean duration of TAF treatment was 18 (3–36) months. In 20 patients with detectable HBV DNA at baseline, median serum levels of HBV DNA log10 changed from 2.33 (0.766–6.47) to 1.04 IU/mL at the end of observation and became undetectable in 11 patients. Median HBsAg log10 decreased from 3.37 (0.88–5.10) to 2.39 (1.52–4.19) IU/mL. During the entire observation period, the renal function parameters remained stable in patients with normal renal function and even in those with renal dysfunction. Mild adverse events were reported by 14 patients (28%). Conclusions: TAF was a safe and effective treatment, also in patients with decreased renal function.
Virus-Specific Nanobody-Chimeras Degrade the Human Cytomegalovirus US28 Protein in CD34+ Cells
Authors: Poole Emma , Schmitt Janika , Graham Stephen C. , Kelly Bernard T. , Sinclair John
Published: September 2024

After primary infection, human cytomegalovirus (HCMV) establishes lifelong persistence, underpinned by latent carriage of the virus with spontaneous reactivation events. In the immune-competent, primary infection or reactivation from latency rarely causes disease. However, HCMV can cause significant disease in immune-compromised individuals such as immune-suppressed transplant patients. Latency, where the viral genome is carried in the absence of the production of infectious virions, can be established in undifferentiated cells of the myeloid lineage. A number of stimuli can cause virus reactivation from latency to occur, beginning with the induction of viral immediate-early (IE) lytic gene expression. The suppression of viral IE gene expression to establish and maintain latent infection is known to result from a balance of viral and cellular factors. One key viral factor involved in this is the G protein-coupled receptor US28. Recently, we have shown that US28 is targeted for degradation by a modified nanobody (PCTD-Vun100bv) based on the novel PACTAC (PCSK9-antibody clearance-targeting chimeras) approach for targeted protein degradation. Furthermore, we have shown that this PCTD-Vun100bv-induced degradation of US28 results in IE gene expression in experimentally latently infected CD14+ monocytes. However, HCMV also establishes latency in CD34+ bone marrow cells, the progenitors of CD14+ cells. Here, we show that PCTD-Vun100bv also causes US28 degradation in these CD34+ primary cells, again resulting in the induction of viral IE gene expression. Additionally, we show that PCTD-Vun100bv can target US28 in naturally latently infected CD14+ monocytes from an HCMV-seropositive donor, allowing these latently infected cells to be killed by HCMV-specific cytotoxic T cells from that same donor. These observations support the view that targeting US28 for degradation during natural latency could be a tractable ‘shock-and-kill’ strategy to target the latent HCMV reservoir in myeloid cells.
Rutin and Physalis peruviana Extract: Population Pharmacokinetics in New Zealand Rabbits
Authors: Domínguez Moré Gina Paola , Rey Diana P. , Valderrama Ivonne H. , Ospina Luis F. , Aragón Diana Marcela
Published: September 2024

Background/Objectives: An extract of calyces from Physalis peruviana with hypoglycemic activity is being considered as a potential herbal medicine. Preclinical pharmacokinetics (PK) studies of the extract in rats, focusing on plasma concentrations of its main compound, rutin, and its metabolites, revealed PK interactions in the extract matrix that improved the absorption of rutin metabolites compared to the pure compound, among other PK effects. This research aimed to study the PK of rutin alone and in the extract and assess potential PK interactions in the extract matrix on the flavonoid and its metabolites in rabbits, a nonrodent species; Methods: Animals received pure rutin or extract orally and intravenously. The PK analysis used noncompartmental and population pharmacokinetics (popPK) methods, and simple allometry was applied to predict human PK parameters; Results: The rutin concentration–time profile fit a two-compartment model with first-order elimination, while its metabolites fit a double first-order absorption model. The extract matrix led to increased absorption, distribution, and elimination of rutin as well as increased bioavailability of its metabolites in rabbits; Conclusions: The popPK model defined the equations for PK parameters describing these findings, and the increased volume of distribution and clearance of rutin was maintained in human predictions. These results will support the development of a new herbal medicine.
Unveiling the Neem (Azadirachta indica) Effects on Biofilm Formation of Food-Borne Bacteria and the Potential Mechanism Using a Molecular Docking Approach
Authors: Mahmoud Ghada Abd-Elmonsef , Rashed Nahed M. , El-Ganainy Sherif M. , Salem Shimaa H.
Published: September 2024

Biofilms currently represent the most prevalent bacterial lifestyle, enabling them to resist environmental stress and antibacterial drugs. Natural antibacterial agents could be a safe solution for controlling bacterial biofilms in food industries without affecting human health and environmental safety. A methanolic extract of Azadirachta indica (neem) leaves was prepared and analyzed using gas chromatography–mass spectrometry for the identification of its phytochemical constituents. Four food-borne bacterial pathogens (Bacillus cereus, Novosphingobium aromaticivorans, Klebsiella pneumoniae, and Serratia marcescens) were tested for biofilm formation qualitatively and quantitatively. The antibacterial and antibiofilm properties of the extract were estimated using liquid cultures and a microtiter plate assay. The biofilm inhibition mechanisms were investigated using a light microscope and molecular docking technique. The methanolic extract contained 45 identified compounds, including fatty acids, ester, phenols, flavonoids, terpenes, steroids, and antioxidants with antimicrobial, anticancer, and anti-inflammatory properties. Substantial antibacterial activity in relation to the extract was recorded, especially at 100 μg/mL against K. pneumoniae and S. marcescens. The extract inhibited biofilm formation at 100 μg/mL by 83.83% (S. marcescens), 73.12% (K. pneumoniae), and 54.4% (N. aromaticivorans). The results indicate efficient biofilm formation by the Gram-negative bacteria S. marcescens, K. pneumoniae, and N. aromaticivorans, giving 0.74, 0.292, and 0.219 OD at 595 nm, respectively, while B. cereus was found to have a low biofilm formation potential, i.e., 0.14 OD at 595 nm. The light microscope technique shows the antibiofilm activities with the biofilm almost disappearing at 75 μg/mL and 100 μg/mL concentrations. This antibiofilm property was attributed to DNA gyrase inhibition as illustrated by the molecular docking approach.
Plant Taxonomy, Systematics and Phylogeography
Authors: Urbaniak Jacek , Kwiatkowski Paweł
Published: September 2024

Plant taxonomy and phylogeny deal with very important problems related to the genetic diversity of populations found in different geographical regions, trying to present the variability of these populations but also to explain their origin. For this reason, such studies examining the conditioned diversity of plant populations were the subject of this Special Issue (SI) of the journal Plants. This SI contains ten original articles and one review article concerning phylogeography and related sciences, i.e., biogeography, taxonomy, and systematics, as well as the genetic variability that lies at the basis of these sciences. The articles included in the SI cover a wide range of topics, including the phylogeny and taxonomy of mosses, taxonomy and hybridization and phylogeny of the genus Chery, genetic diversity and phylogeography of fern, including relict species from the Macronesia area, taxonomy of the genus Aconitum, a species of orchid new to the Romanian flora, but also a proposal for a new phylogeographic division in Gansu Province (China) and a review of the role of the Hercynian Mountains in Shaping Plant Migration Patterns in the Pleistocene.
Brazil Nut Semi-Defatted Flour Oil: Impact of Extraction Using Pressurized Solvents on Lipid Profile, Bioactive Compounds Composition, and Oxidative Stability
Authors: Barbosa Abrantes Karen Keli , Colombo Pimentel Tatiana , da Silva Camila , Santos Junior Oscar de Oliveira , Barão Carlos Eduardo , Cardozo-Filho Lucio
Published: September 2024

Brazilian nuts are native to the Amazon rainforest and are considered a non-timber forest-product of extreme economic importance to local populations. This study evaluated the lipid profile, bioactive compounds, and oxidative stability of semi-defatted Brazilian nut flour oil (BNSDFO) obtained using pressurized fluids (n-propane at 40 °C and 2, 4, and 8 MPa or a CO2/n-propane mixture at 40 °C and 12 MPa). A Brazilian nut kernel oil (BNKO) processed by conventional cold pressing was also obtained. The BNKO showed a higher concentration of total phenolic compounds and saturated fatty acids, higher antioxidant activity, and the presence of gallic acid derivatives. The oils extracted using pressurized fluids showed a higher concentration of linoleic acid, β-sitosterol, and polyunsaturated fatty acids. The utilization of pressurized n-propane resulted in higher yields (13.7 wt%), and at intermediate pressures (4 MPa), the product showed myricetin 3-O-rhamnoside and higher oxidative stability (OSI, 12 h) than at lower pressures (2 MPa). The CO2/n-propane mixture of pressurized solvents resulted in higher concentrations of squalene (4.5 times), the presence of different phenolic compounds, and a high OSI (12 h) but lower yield (2.2 wt%). In conclusion, oils with better fatty acid profiles (oleic e linoleic acids), phytosterol composition, and suitable radical scavenging activity may be obtained using pressurized fluids and Brazilian nut flour, a byproduct of oil extraction. The mixture of solvents may improve the concentration of squalene, whereas using only n-propane may increase oil yield.