The Pharmacological Study of Tubercle Bacilli
Authors: Yu Pyung Suh 
Published: April 2021

Introduction: Many scholars have accomplished a lot of valuable work about tubercle bacilli. but little work has been done pharmacologically on the toxic reaction of tubercle bacilli.Ⅰ. Culture of Tubucle bacillus: B. C. G. stocks in the Sauton' medium were used. Ⅱ. Method: 1. Method of manufacturing dead tubercle bacillus to XIn. 2. Method of manufacturing living tubercle bacillus toxin. 3. Method of manufacturing tubercle bacillus culture liltrate.Ⅲ. Toxicity test of tubercle bacillus towards the mouse. Ⅳ . Oral immunity test of B.C.G.: Oral immunity was obtained, but it was not so successful as in the case of intracutaneous injection.Ⅴ. Inoculation test of avian type tubercle bacillus towards the domestic fowl. Inoculation has been done only with living bacillis. Ⅵ. Inoculation test of B.C.G. culture filtrate towards the guinea Pig. : On observing the inoculated animals. it was found that Romel’s reaction was negative.Ⅶ. Research of the factor which makes Tuberculin reaction positive. It has been found that the factor which makes Tuberculin reaction Positive is not in the B.C.G. culture filtrate.Ⅷ . The toxicity of the avian type tubercle bacillus towards the ginea pig. Avian type Tuberculin bacillus has little toxicity towards gunea pigs.Ⅸ . The influence of the avian type tubercle bacillus culture filtrate on the R.B.C. sedimentation rate of the domestic fowl: The rate has peen accelerated. When heated, there has been no change.Ⅹ. Pharmacological action of the toxin of tubercle bacill. (Tried this experiment on the 10 animals respect lively. )1. Action on the coronary blood vessels of the rabbit by Langendorff-.Naito’s method Was also observed. Action on the pulmonary vessels of the toad.: In the pulmonary circulation of the toad, pressure of the fluid (fluid of culture filtrate) has been made to about 10 cm, and irrigatin has been done. Result was also contraction of the blood vessels. Action on the leg blood vessels of the toad.: There was not reaction. Action on the mesenteric blood vessels of the toad.: There was not reaction. A tion on the mesenteric blood vessels of the toad.: Result was contraction of the mesenteric blood vessels.2. Action on the intestinal canal. Action the small intestine of the rabbit.: Magnus method was used, the result weakening of the small intestine peristaltic movement. Action on the small intestine of the domestic fowl. : Magnus' method. The result was' as above. Action on the intestinal canal of the mouse.: The method was the same. consequently the tension was lowered. Action on the isolated intestine of the guinea pig.: Method and result were the same. Action on the isolated intestine of the cat. : Method and result were the same. Action on the isolated intestine of the toad. : The method was the same, but there was no reaction.3. Action on the isolated uterus. Action on the the isolated uterus of the mouse The method was Magnus .The contraction increased. Action on the isolated oviduct ot the toad.: The method was the same and no special reaction was noted.Conclusion : 1. The toxicty of the toxin of B.C.G. and avian type tubercle bacillus has not been proved on my experiments.2. Although it is possible to produce tuberculous immunity by oral administration, the result following absorption in the intestine seems to be different from that produced by intracutaneous injection.3. Bacteriological and pharmacological activities of B.C.G. and avian type tubercle bacilli are different.4. In the culture filtrates oÍ both, no factor which makes Tuberculin test positive is found, and the culture filtrate of the avian type tubercle bacillus seems to have a certain substance which makes the sedimentation rate of the domestic fowl accelerated. The above substance has not been found to de toxic to the guinea píg. 5. pharmacologocal speaking. in the calture filtrate of tubucle bacilli, there seems to exist a certain substance which acts activity in every organ of the Jiving body. This must he something excreted from the bacillus while the tubercle bacilli are living.6. This substance has strong power of resistance to heat, and the toxicity seems to be weakened by irrigating the liver. (supposed by the liver action)7. lt is not certain that this substance is the immunogen of tubercle bacilli.