bluuebunny/crossref_metadata_2025 huggingface dataset:
Mind over Microplastics: Exploring Microplastic-Induced Gut Disruption and Gut-Brain-Axis Consequences
Authors: Sofield Charlotte E. , Anderton Ryan S. , Gorecki Anastazja M.
Published: May 2024

As environmental plastic waste degrades, it creates an abundance of diverse microplastic particles. Consequently, microplastics contaminate drinking water and many staple food products, meaning the oral ingestion of microplastics is an important exposure route for the human population. Microplastics have long been considered inert, however their ability to promote microbial dysbiosis as well as gut inflammation and dysfunction suggests they are more noxious than first thought. More alarmingly, there is evidence for microplastics permeating from the gut throughout the body, with adverse effects on the immune and nervous systems. Coupled with the now-accepted role of the gut-brain axis in neurodegeneration, these findings support the hypothesis that this ubiquitous environmental pollutant is contributing to the rising incidence of neurodegenerative diseases, like Alzheimer’s disease and Parkinson’s disease. This comprehensive narrative review explores the consequences of oral microplastic exposure on the gut-brain-axis by considering current evidence for gastrointestinal uptake and disruption, immune activation, translocation throughout the body, and neurological effects. As microplastics are now a permanent feature of the global environment, understanding their effects on the gut, brain, and whole body will facilitate critical further research and inform policy changes aimed at reducing any adverse consequences.
Salivary Diagnosis of Dental Caries: A Systematic Review
Authors: Antonelli Rita , Massei Valentina , Ferrari Elena , Gallo Mariana , Pertinhez Thelma A. , Vescovi Paolo , Pizzi Silvia , Meleti Marco
Published: May 2024

The activity of dental caries, combined with its multifactorial etiology, alters salivary molecule composition. The present systematic review was developed to answer the following question: “Are salivary biomarkers reliable for diagnosis of dental caries?”. Following the “Preferred Reporting Item for Systematic Reviews and Meta-analysis” (PRISMA) guidelines, the review was conducted using multiple database research (Medline, Web of Science, and Scopus). Studies performed on healthy subjects with and without dental caries and providing detailed information concerning the clinical diagnosis of caries (Decayed, Missing, Filled Teeth-DMFT and International Caries Detection and Assessment System-ICDAS criteria) were included. The quality assessment was performed following a modified version of the Joanna Briggs Institute Prevalence Critical Appraisal Checklist. The protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO, ID: CRD42022304505). Sixteen papers were included in the review. All studies reported statistically significant differences in the concentration of salivary molecules between subjects with and without caries (p < 0.05). Proteins were the most investigated molecules, in particular alpha-amylase and mucins. Some studies present a risk of bias, such as identifying confounding factors and clearly defining the source population. Nevertheless, the 16 papers were judged to be of moderate to high quality. There is evidence that some salivary compounds studied in this review could play an important diagnostic role for dental caries, such as salivary mucins, glycoproteins (sCD14), interleukins (IL-2RA, 4,-13), urease, carbonic anhydrase VI, and urea.
The Role of Galectins in Asthma Pathophysiology: A Comprehensive Review
Authors: Portacci Andrea , Iorillo Ilaria , Maselli Leonardo , Amendolara Monica , Quaranta Vitaliano Nicola , Dragonieri Silvano , Carpagnano Giovanna Elisiana
Published: May 2024

Galectins are a group of β-galactoside-binding proteins with several roles in immune response, cellular adhesion, and inflammation development. Current evidence suggest that these proteins could play a crucial role in many respiratory diseases such as pulmonary fibrosis, lung cancer, and respiratory infections. From this standpoint, an increasing body of evidence have recognized galectins as potential biomarkers involved in several aspects of asthma pathophysiology. Among them, galectin-3 (Gal-3), galectin-9 (Gal-9), and galectin-10 (Gal-10) are the most extensively studied in human and animal asthma models. These galectins can affect T helper 2 (Th2) and non-Th2 inflammation, mucus production, airway responsiveness, and bronchial remodeling. Nevertheless, while higher Gal-3 and Gal-9 concentrations are associated with a stronger degree of Th-2 phlogosis, Gal-10, which forms Charcot–Leyden Crystals (CLCs), correlates with sputum eosinophilic count, interleukin-5 (IL-5) production, and immunoglobulin E (IgE) secretion. Finally, several galectins have shown potential in clinical response monitoring after inhaled corticosteroids (ICS) and biologic therapies, confirming their potential role as reliable biomarkers in patients with asthma.
Exploring Maternal Diet-Epigenetic-Gut Microbiome Crosstalk as an Intervention Strategy to Counter Early Obesity Programming
Authors: Faienza Maria Felicia , Urbano Flavia , Anaclerio Federico , Moscogiuri Luigi Antonio , Konstantinidou Fani , Stuppia Liborio , Gatta Valentina
Published: May 2024

Alterations in a mother’s metabolism and endocrine system, due to unbalanced nutrition, may increase the risk of both metabolic and non-metabolic disorders in the offspring’s childhood and adulthood. The risk of obesity in the offspring can be determined by the interplay between maternal nutrition and lifestyle, intrauterine environment, epigenetic modifications, and early postnatal factors. Several studies have indicated that the fetal bowel begins to colonize before birth and that, during birth and nursing, the gut microbiota continues to change. The mother’s gut microbiota is primarily transferred to the fetus through maternal nutrition and the environment. In this way, it is able to impact the establishment of the early fetal and neonatal microbiome, resulting in epigenetic signatures that can possibly predispose the offspring to the development of obesity in later life. However, antioxidants and exercise in the mother have been shown to improve the offspring’s metabolism, with improvements in leptin, triglycerides, adiponectin, and insulin resistance, as well as in the fetal birth weight through epigenetic mechanisms. Therefore, in this extensive literature review, we aimed to investigate the relationship between maternal diet, epigenetics, and gut microbiota in order to expand on current knowledge and identify novel potential preventative strategies for lowering the risk of obesity in children and adults.
A Case for Trans-Regional Intertidal Research in Unstudied Areas in the Northeast and Southeast Pacific: Filling the Gaps
Authors: Wilbur Lynn , Louca Vasilis , Ibanez-Erquiaga Bruno , Küpper Frithjof C.
Published: May 2024

Large areas of the Pacific coast of the Americas remain unstudied regarding their intertidal ecosystems. Given the increasing disturbance related to human impacts on intertidal ecosystems, it is essential to gather census data on the biological composition of poorly studied regions so that a framework for future monitoring and management can be developed. Here, we synthesize the available research on intertidal communities along the Pacific rim to support the goal to fill bioregional gaps in knowledge in three less-studied areas in Southeast Alaska and Peru. A census of taxonomic and functional group hierarchies in these communities should include the use of various measures of alpha and beta diversity to provide a metric of temporal and spatial comparisons. A narrower-scale approach focusing on foundation species that harbor algal and invertebrate communities and serve as buffers against environmental stresses should also be conducted. Conducting a comprehensive census in poorly studied or unstudied areas will contribute to a better understanding of the response to disturbances caused by oil spills, El Niño and marine heatwaves and provide a latitudinal continuum of scientific knowledge about the biodiversity and ecosystem functioning in rocky intertidal systems on a trans-regional scale.
Accelerated Fractionated Radiation Therapy for Localized Glottic Carcinoma
Authors: Mizukami Tatsuji , Yamagishi Kentaro , Tobikawa Masaki , Nakazato Akira , Abe Hideharu , Morita Yuka , Saitoh Jun-ichi
Published: May 2024

Background: The aim of this study is to examine the outcomes of an accelerated fractionated irradiation for N0 glottic carcinoma. Methods: In this retrospective analysis, 29 patients with N0 glottic carcinoma treated by radiation therapy were enrolled. Thirteen patients had T1a disease, six had T1b disease, and ten had T2 disease. A fractional dose of 2.1 Gy was administered to seven patients. The total doses were 65.1 and 67.2 Gy in four and three patients, respectively. A fractional dose of 2.25 Gy was administered to 22 patients. The total doses were 63 and 67.5 Gy in 21 patients and 1 patient with T2 disease, respectively. Additionally, 13 patients underwent the use of TS-1 (80–100 mg per day). Results: The median follow-up period was 33 months, and the 3-year local control rate was 95.6%. No patient had a lymph node or distant recurrence. As acute adverse events, grades 2 and 3 dermatitis were observed in 18 patients and 1 patient, and grades 2 and 3 mucositis were observed in 15 patients and 1 patient. As a late adverse event, one patient required tracheotomy because of laryngeal edema occurring. Conclusions: Accelerated fractionated irradiation may be an option in the radiation therapy of N0 glottic carcinoma because of its ability to shorten the treatment time.
Improving Target Geolocation Accuracy with Multi-View Aerial Images in Long-Range Oblique Photography
Authors: Liu Chongyang , Ding Yalin , Zhang Hongwen , Xiu Jihong , Kuang Haipeng
Published: May 2024

Target geolocation in long-range oblique photography (LOROP) is a challenging study due to the fact that measurement errors become more evident with increasing shooting distance, significantly affecting the calculation results. This paper introduces a novel high-accuracy target geolocation method based on multi-view observations. Unlike the usual target geolocation methods, which heavily depend on the accuracy of GNSS (Global Navigation Satellite System) and INS (Inertial Navigation System), the proposed method overcomes these limitations and demonstrates an enhanced effectiveness by utilizing multiple aerial images captured at different locations without any additional supplementary information. In order to achieve this goal, camera optimization is performed to minimize the errors measured by GNSS and INS sensors. We first use feature matching between the images to acquire the matched keypoints, which determines the pixel coordinates of the landmarks in different images. A map-building process is then performed to obtain the spatial positions of these landmarks. With the initial guesses of landmarks, bundle adjustment is used to optimize the camera parameters and the spatial positions of the landmarks. After the camera optimization, a geolocation method based on line-of-sight (LOS) is used to calculate the target geolocation based on the optimized camera parameters. The proposed method is validated through simulation and an experiment utilizing unmanned aerial vehicle (UAV) images, demonstrating its efficiency, robustness, and ability to achieve high-accuracy target geolocation.
Vision-Guided Tracking and Emergency Landing for UAVs on Moving Targets
Authors: Debele Yisak , Shi Ha-Young , Wondosen Assefinew , Warku Henok , Ku Tae-Wan , Kang Beom-Soo
Published: May 2024

This paper presents a vision-based adaptive tracking and landing method for multirotor Unmanned Aerial Vehicles (UAVs), designed for safe recovery amid propulsion system failures that reduce maneuverability and responsiveness. The method addresses challenges posed by external disturbances such as wind and agile target movements, specifically, by considering maneuverability and control limitations caused by propulsion system failures. Building on our previous research in actuator fault detection and tolerance, our approach employs a modified adaptive pure pursuit guidance technique with an extra adaptation parameter to account for reduced maneuverability, thus ensuring safe tracking of moving objects. Additionally, we present an adaptive landing strategy that adapts to tracking deviations and minimizes off-target landings caused by lateral tracking errors and delayed responses, using a lateral offset-dependent vertical velocity control. Our system employs vision-based tag detection to ascertain the position of the Unmanned Ground Vehicle (UGV) in relation to the UAV. We implemented this system in a mid-mission emergency landing scenario, which includes actuator health monitoring of emergency landings. Extensive testing and simulations demonstrate the effectiveness of our approach, significantly advancing the development of safe tracking and emergency landing methods for UAVs with compromised control authority due to actuator failures.
Chaos Synchronization of Integrated Five-Section Semiconductor Lasers
Authors: Guo Yuanyuan , Du Yao , Gao Hua , Tan Min , Zhao Tong , Jia Zhiwei , Chang Pengfa , Wang Longsheng
Published: May 2024

We proposed and verified a scheme of chaos synchronization for integrated five-section semiconductor lasers with matching parameters. The simulation results demonstrated that the integrated five-section semiconductor laser could generate a chaotic signal within a large parameter range of the driving currents of five sections. Subsequently, chaos synchronization between two integrated five-section semiconductor lasers with matched parameters was realized by using a common noise signal as a driver. Moreover, it was found that the synchronization was sensitive to the current mismatch in all five sections, indicating that the driving currents of the five sections could be used as keys of chaotic optical communication. Therefore, this synchronization scheme provides a candidate to increase the dimension of key space and enhances the security of the system.
Development of Definitory and Classificatory Thinking in Geometry through Storytelling and GBL Activities
Authors: Tortorelli Leonardo , Tortoriello Francesco Saverio
Published: May 2024

Little is discussed regarding the meaning of “definition” in primary school, where children often memorize definitions. In geometry, students frequently confuse “definition” with the “description” of a geometric object. The aim of this study is to verify whether a new hybrid methodology between storytelling and game-based learning called Geometrikoland exercises skills that fall within what we understand as “definitory thinking”, which the set of competencies and meta-competencies is associated with acquiring a true mastery of definitions in geometry. This goal can be achieved because the dynamics of Geometrikoland alter the approach to definitions. The methodology applied is a hybrid of storytelling and adventure game-based learning applied to a “engine” of quadrilateral theory. The experiment took place in a fifth-grade class in an Italian school. The analysis of some data based on the accuracy of geometry actions during workshops and qualitative analysis based on the analysis of oral arguments produced during the labs lead to the conclusion that, on average, children have gained a greater awareness of what it means to define a geometric object and a better understanding of the relationships between various subsets of the set of quadrilaterals, previously seen as separate sets. Further confirmation has been provided by comparing the results of two mini-tests (a placement test and an exit test), which are useful for assessing the skills associated with the criterion of arbitrariness, the criterion of uniqueness, and classificatory thinking.
Assessing Learning in an Immersive Virtual Reality: A Curriculum-Based Experiment in Chemistry Education
Authors: Qorbani Sam , Dalili Shadi , Arya Ali , Joslin Christopher
Published: May 2024

Despite the recent advances in Virtual Reality technology and its use in education, the review of the literature shows several gaps in research on how immersive virtual environments impact the learning process. In particular, the lack of curriculum-specific experiments along with investigations of the effects of different content, activity, and interaction types in the current VR studies has been identified as a significant shortcoming. This has been more significant in STEM fields, where VR has the potential to offer engaging experiential learning opportunities. The study reported here was designed to address this gap by assessing the effect of authentic visualization and interaction types on learning a particular scientific concept. A use case scenario of “orbital hybridization” in chemistry education was selected to create this experiment and to collect data for analysis. We collected data on learning outcomes, task-completion efficiency, accuracy, and subjective usability. A combination of learning content and tasks designed based on the relevant educational theories was presented to three groups: 2D, VR interaction type 1 (hand gestures), and VR interaction type 2 (ray casting). The results showed that VR could improve learning and that interaction type could influence efficiency and accuracy depending on the task.
‘Alone in a Crowd’: Teacher-Level and Pupil-Level Hidden Curricula and the Theoretical Limits of Teacher–Pupil Relationships
Authors: Whittaker Daniel
Published: May 2024

This essay aims to explore the theoretical limitations that hidden curricula—hidden normative values, beliefs, and knowledge that are often considered problematic—place on our understanding of teacher–pupil relationships. It applies Habermas’ theory of communicative action—synonymous with mutual understanding and predicated on his concept of the lifeworld—to analyse hidden curricula. It finds that hidden curricula limit teachers’ comprehension of teacher–pupil relationships dependent on pupils’ responses to teacher-level hidden curricula. Where they respond with compliance, pupils limit expressions of their subjectivity; conversely, where they reject teacher-level hidden curricula, pupils’ subjective lifeworlds are already disrupted by them. Both responses impede teachers’ understanding of teacher–pupil relationships. In addition, pupil-level hidden curricula, which are often asymmetrical and oriented in response to teacher-level hidden curricula, present another barrier to teachers unveiling hidden curricula and the subjectivities of teacher–pupil relationships. In effect, pupil-level hidden curricula render teachers ‘alone in a crowd’. Finally, I argue that systematically examining hidden curricula represents strategic action—communicative action’s counterpoint—and colonisation of pupils’ lifeworlds. While hidden curricula present significant theoretical limitations to understanding teacher–pupil relationships, teachers might use this as a ‘pedagogical hinge’, freeing them from the unknowable and uncontrollable to a more practical view of teacher–pupil relationships.
Understanding School Middle-Leading Practices: Developing a Middle-Leading Practice Model
Authors: Tindall-Ford Sharon , Grootenboer Peter , Edwards-Groves Christine , Attard Catherine
Published: May 2024

School systems in Australia, and internationally, are focused on improving classroom teaching and learning to enhance student outcomes. Middle leaders (MLs) are increasingly required to lead school-based development initiatives to improve classroom practices. Informed by previous research on middle-leading and the theory of practice architectures, a survey instrument was created to understand who Australian school MLs are (n = 199) and ascertain their perceptions of the practices central to leading teaching and learning in their school sites. Through descriptive, exploratory, and confirmatory factor analyses, this paper reports on the reported practices of Australian MLs, and, through the analysis, a revised ML practice model is proposed. The results confirm that ML practices are orientated to the people they lead in their school site, who they support, collaborate with, and advocate for, with practising leading upwards to the school principal identified as an important ML practice.
A Genealogy toward Methodic Doubts in Educational Leadership Research
Authors: Bogotch Ira
Published: May 2024

In this critical narrative essay, titled A Genealogy Toward Methodic Doubts, I ask readers not only how and why educational leadership research(ers) detoured from the rigorous pursuits of truths, but also how and why I had missed the signposts which might have alerted me and others that our continuing practices, even with recognizing methodological limitations, were flawed. The empirical examples presented here come mostly from US policies, Western theories, and traditional methods, but likely apply to other contexts worldwide.
Associations of eHealth Literacy with Social Activity among Community-Dwelling Older Adults: A Cross-Sectional Study
Authors: Eto Mamiko , Yamatsu Koji
Published: May 2024

Older adults who use digital technology are desired to adapt to digitalization and literacy. One required aspect is eHealth literacy, measured with the eHealth Literacy Scale (eHEALS). Many studies on eHealth literacy have used the eHEALS to examine the health behaviors of college students, relatively younger adults, and Internet users. However, the relevance of eHealth literacy to social activity has not yet been studied in older adults. The purposes of this study were to examine the relationship between eHealth literacy and health behaviors and social activities (community, cultural, and sports activities) and to investigate the factors associated with eHEALS scores among community-dwelling older adults. The mean eHEALS score was 12.4 points (SD 8.2), with the majority (73.3%) having the lowest score (the lowest score is 8 points). Males (17.6, SD 10.5) scored significantly higher than females (11.8, SD 7.7). The eHEALS score had a significant relationship with both cultural and community activity. Five factors significantly associated with having the lowest eHEALS score were cultural activity at least once a week, no cultural activity, no community activity, total IADL score, and intellectual activity. These results suggest that eHealth literacy is associated with community activity and cultural activity among older adults.
Optimized and Sustainable PV Water Pumping System with Three-Port Converter, a Case Study: The Al-Kharijah Oasis
Authors: Selmy Mohamed , El sherif Mohsen Z. , Noah Miral Salah , Abdelqawee Islam M.
Published: May 2024

In this paper an efficient, compact, and cheap power source design for an off-grid PV water pumping system is investigated. The proposed system consists of a PV array, battery, three-port converter (TPC), three-phase voltage source inverter, and induction motor pump. Power is extracted from PV sources during the daytime and used to charge batteries through the three-port converter, then used later to supply load during the nighttime. An intelligent MPPT method is used to obtain PV maximum power; a jellyfish optimization technique with different control algorithms is used to optimize and tune controllers’ parameters among the system. Different modes for the TPC are discussed depending on PV power availability. The proposed system is simulated to assess system performance under different conditions; also the system is efficient with reduced number of components than conventional converters. A complete unified power management over PV input port, battery port, and load port has occurred for all operation modes. At all operation modes, the system has been feeding load without any unmet loads. A real case study in Al-Kharijah oasis is studied and simulation results are listed; for the Dom case DC bus ripple factor voltage percentage equals 0.8%, in the Dim case equals 3%, and in the Siso mode equals 9%.
FedSKF: Selective Knowledge Fusion via Optimal Transport in Federated Class Incremental Learning
Authors: Zhou Minghui , Wang Xiangfeng
Published: May 2024

Federated learning has been a hot topic in the field of artificial intelligence in recent years due to its distributed nature and emphasis on privacy protection. To better align with real-world scenarios, federated class incremental learning (FCIL) has emerged as a new research trend, but it faces challenges such as heterogeneous data, catastrophic forgetting, and inter-client interference. However, most existing methods enhance model performance at the expense of privacy, such as uploading prototypes or samples, which violates the basic principle of only transmitting models in federated learning. This paper presents a novel selective knowledge fusion (FedSKF) model to address data heterogeneity and inter-client interference without sacrificing any privacy. Specifically, this paper introduces a PIT (projection in turn) module on the server side to indirectly recover client data distribution information through optimal transport. Subsequently, to reduce inter-client interference, knowledge of the global model is selectively absorbed via knowledge distillation and an incomplete synchronization classifier at the client side, namely an SKS (selective knowledge synchronization) module. Furthermore, to mitigate global catastrophic forgetting, a global forgetting loss is proposed to distill knowledge from the old global model. Our framework can easily integrate various CIL methods, allowing it to adapt to application scenarios with varying privacy requirements. We conducted extensive experiments on CIFAR100 and Tiny-ImageNet datasets, and the performance of our method surpasses existing works.
Disturbance Observer-Based Tracking Controller for n-Link Flexible-Joint Robots Subject to Time-Varying State Constraints
Authors: Zhang Zhongcai , Hu Xueli , Huang Peng
Published: May 2024

This paper addresses the tracking control for an n-link flexible-joint robot system with full-state constraints and external disturbances. First, a nonlinear disturbance observer (NDO) is introduced to asymptotically estimate and suppress the influence of the related disturbances. Next, the constrained system under consideration is transformed into a new unconstrained system using state-dependent function (SDF) transformations. Subsequently, a NDO-based tracking controller that combines the backstepping method and filter technique is proposed in this work. Based on stability analysis, it can be proven that the tracking error converges to a predefined compact set, which can be arbitrarily small without violating the full-state constraints. Finally, simulation results are presented to demonstrate the validity of the suggested control algorithm.
Improving Scanning Performance of Patch Phased Array Antenna by Using a Modified SIW Cavity and Sequential Rotation Technique
Authors: Liu Hao , Guan Tianci , Fu Chunsen , Zhang Shuqi , Xu Xin , Xu Ziqiang , Qing Anyong , Lin Xianqi
Published: May 2024

A novel patch phased array antenna with improved scanning performance is presented in this paper. The active element pattern is changed as desired through a modified SIW cavity, resulting in an extension of the phased array’s 3 dB scanning range. Furthermore, sequential rotation is used to reduce the cross-polarization level of the array, which also improves the scanning gain at ±45°. Without altering the element size or profile, the array has the merits of low cost, low complexity, and a simple feed structure. The presented phased array antenna (PAA) exhibits a gain fluctuation of less than 2.2 dB when steering to 45°. Furthermore, the cross-polarization levels are below −68.1 dB when scanning to 45° in a E-/H-plane over the whole working band. To validate the proposed design, a prototype of a 24 × 16 active PAA is designed, fabricated, and measured. A good agreement between the simulated and measured results is achieved, Thus, this paper offers a viable solution to enhance the scanning performance of a PAA with fixed interelement spacing.
Local-Global Representation Enhancement for Multi-View Graph Clustering
Authors: Zhao Xingwang , Hou Zhedong , Wang Jie
Published: May 2024

In recent years, multi-view graph clustering algorithms based on representations learning have received extensive attention. However, existing algorithms are still limited in two main aspects, first, most algorithms employ graph convolution networks to learn the local representations, but the presence of high-frequency noise in these representations limits the clustering performance. Second, in the process of constructing a global representation based on the local representations, most algorithms focus on the consistency of each view while ignoring complementarity, resulting in lower representation quality. To address the aforementioned issues, a local-global representation enhancement for multi-view graph clustering algorithm is proposed in this paper. First, the low-frequency signals in the local representations are enhanced by a low-pass graph encoder, which yields smoother and more suitable local representations for clustering. Second, by introducing an attention mechanism, the local embedded representations of each view can be weighted and fused to obtain a global representation. Finally, to enhance the quality of the global representation, it is jointly optimized using the neighborhood contrastive loss and reconstruction loss. The final clustering results are obtained by applying the k-means algorithm to the global representation. A wealth of experiments have validated the effectiveness and robustness of the proposed algorithm.
Qualitative Changes in Birch Sap after Freezing and Thawing
Authors: Mingaila Justas , Vilimas Vladas , Viškelis Pranas , Marozas Vitas , Bobinas Česlovas , Viškelis Jonas
Published: May 2024

In this study, the qualitative changes in raw birch sap after freezing and thawing were determined. Ten-liter bottles and one-ton plastic containers with six replications were used for the freezing of birch sap and thawing of frozen sap. During and after the thawing, the physical and physical–chemical properties of the sap were measured. According to the results, as the ice melts, the concentration of acids and other soluble substances in the sap decreases, but changes in qualitative indicators indicate the beginning of fermentation processes through color changes and pH as the temperature of the melting sap becomes positive. As a result, to freeze raw sap in large-volume containers, it is necessary to develop fast thawing technology using auxiliary means—circulation, external energy sources, and mechanical ice crushing.
Effect of Toxicity of Chromium (VI) Stressors Alone and Combined to High Temperature on the Histopathological, Antioxidation, Immunity, and Energy Metabolism in Fish Phoxinus lagowskii
Authors: Hu Tingting , Ye Cunrun , Ning Zhaoyang , Liu Tianmei , Mu Weijie
Published: May 2024

Fish in aquatic ecosystems are often impacted by environmental stressors like temperature fluctuations and exposure to heavy metals. Chromium (Cr6+) is a known environmental pollutant that poses a threat to aquatic life. Various environmental factors, such as water temperature, have been found to affect the toxicity of dissolved chemicals in aquatic ecosystems. We investigated the toxicity of combinations of different concentrations of hexavalent chromium (Cr6+) with high temperatures in fish. Hematological indices demonstrated changes in white blood cells (WBCs), hematocrit (HCT), red blood cells (RBCs), and hemoglobin (Hb) levels during the exposure. The qualitative and semi-quantitative analyses of different tissues confirmed that higher concentrations of Cr6+ caused more significant damage than lower concentrations, with evident alterations observed in circulatory and regressive aspects. Furthermore, brain acetylcholinesterase levels decreased in both single heavy metal exposure and combined exposure at a high temperature. The activity of antioxidant oxidase and immunological parameters increased in all treatment groups compared with the control group following long-term exposure. A significant and increased effect of Cr6+ in the high-temperature groups was observed on the evaluated biomarkers, suggesting a possible synergistic effect between Cr6+ and increased temperature. The integrated biomarker response (IBR) reported the highest level of stress at 10 mg/L Cr6+ combined with high temperature. The IBR analysis revealed that the highest activity of response enzymes, such as acid phosphatase (ACP), superoxide dismutase (SOD), and glutathione S-transferases (GST), was observed in the liver, whereas the gills displayed alkaline phosphatase (ALP), GST, and SOD activity, and the kidneys demonstrated SOD, ACP, and aspartate aminotransferase (AST) to be most active. Through histopathology, antioxidant enzymes, and metabolism- and immunity-related enzymes, we determined that high temperatures enhance the potential toxicity of Cr6+ in fish. We recommend conducting a thorough assessment of the impact of climate change, particularly temperature fluctuations, when studying the toxic effects of metal pollution, like chromium, in aquatic ecosystems.
Study of Orifice Design on Oleo-Pneumatic Shock Absorber
Authors: Silva Paulo A. S. F. , Sheikh Al-Shabab Ahmed A. , Tsoutsanis Panagiotis , Skote Martin
Published: May 2024

Aircraft oil-strut shock absorbers rely on orifice designs to control fluid flow and optimize damping performance. However, the complex nature of cavitating flows poses significant challenges in predicting the influence of orifice geometry on energy dissipation and system reliability. This study presents a comprehensive computational fluid dynamics (CFD) analysis of the effects of circular, rectangular, semicircular, and cutback orifice profiles on the internal flow characteristics and damping behavior of oleo-pneumatic shock absorbers. High-fidelity simulations reveal that the rectangular orifice generates higher damping pressures and velocity magnitude than those generated by others designs, while the semicircular shape reduces cavitation inception and exhibits a more gradual pressure recovery. Furthermore, the study highlights the importance of considering both geometric and thermodynamic factors in the design and analysis of cavitating flow systems, as liquid properties and vapor pressure significantly impact bubble growth and collapse behavior. Increasing the orifice length had a negligible impact on damping but moderately raised orifice velocities. This research provides valuable insights for optimizing shock absorber performance across a range of operating conditions, ultimately enhancing vehicle safety and passenger comfort.
Current Research on Probiotics and Fermented Products
Authors: Dong Yushi , Li Mohan , Yue Xiqing
Published: May 2024

The history of probiotics and fermented products has evolved over millennia [...]
Assessment of Anti-Prostate Cancer Activity among Four Seaweeds, with Focus on Caulerpa lentillifera J.Agardh
Authors: Wu Guan-James , Hsiao Pei-Wen
Published: May 2024

In response to a global shift towards health-conscious and environmentally sustainable food choices, seaweed has emerged as a focus for researchers due to its large-scale cultivation potential and the development of bioactive substances. This research explores the potential anticancer properties of seaweed extracts, focusing on analyzing the impact of four common edible seaweeds in Taiwan on prostate cancer (PCa) cells’ activity. The study used bioassay-guided fractionation to extract Cl80 from various seaweeds with androgen receptor (AR)-inhibitory activity. Cl80 demonstrated effective suppression of 5α-dihydrotestosterone (DHT)-induced AR activity in 103E cells and attenuated the growth and prostate-specific antigen (PSA) protein expression in LNCaP and 22Rv1 cells. Additionally, Cl80 exhibited differential effects on various PCa cell lines. Concentrations above 5 μg/mL significantly inhibited LNCaP cell proliferation, while 22Rv1 cells were more resistant to Cl80. PC-3 cell proliferation was inhibited at 5 μg/mL but not completely at 50 μg/mL. A clonogenic assay showed that at a concentration of 0.5 μg/mL, the colony formation in LNCaP and PC-3 cells was significantly reduced, with a dose-dependent effect. Cl80 induced apoptosis in all PCa cell types, especially in LNCaP cells, with increased apoptotic cells observed at higher concentrations. Cl80 also decreased the mitochondrial membrane potential (ΔΨm) in a dose-dependent manner in all PCa cell lines. Furthermore, Cl80 suppressed the migration ability of PCa cells, with significant reductions observed in LNCaP, 22Rv1, and PC-3 cells at various concentrations. These compelling findings highlight the promising therapeutic potential of C. lentillifera J.Agardh and its isolated compound Cl80 in the treatment of PCa.
Evaluating Attitudes and Preferences towards Walking in Two European Cities
Authors: Fonseca Fernando , Papageorgiou George , Conticelli Elisa , Jabbari Mona , Ribeiro Paulo J. G. , Tondelli Simona , Ramos Rui
Published: May 2024

Understanding pedestrian perceptions and attitudes is crucial for promoting walking as a daily transportation mode for sustainable mobility and the effective development of smart cities. Pedestrian preferences, shaped by factors such as age, gender, and urban infrastructure, play a pivotal role in travel behaviors. Based on a survey study, this paper examines the impact of individual and urban factors on pedestrian perceptions and attitudes towards walking in the cities of Bologna and Porto. Results reveal that individuals generally value short, safe, and green walking routes, appreciating walking for physical activity, cost savings, and time efficiency. Disliked aspects include adverse weather conditions and walking on inadequate sidewalks. Through carrying out Chi-square statistical analysis tests, a variety of significant correlations between individual and urban variables have been revealed on what people like or dislike about walking. For instance, males, young individuals, and students were more likely to prefer short pedestrian routes, while adults and seniors favored safe and green routes. These findings can assist urban planners in identifying factors that make walking both convenient and enjoyable and in supporting sustainable urban mobility policies.
Production and Physicochemical Characterization of the Gel Obtained in the Fermentation Process of Blue Corn Flour (Zea mays L.) with Colletotrichum gloeosporioides
Authors: Villarreal-Rodríguez Guadalupe , Escajeda-García Jesús Manuel , Chen Lingyun , Amaya-Olivas Nubia , Ruiz-Anchondo Teresita , Neder-Suarez David , Chávez-Flores David , Gutierrez-Mendez Néstor , Hernández-Ochoa León
Published: May 2024

Food gels are viscoelastic substances used in various gelled products manufactured around the world. Polysaccharides are the most common food gelling agents. The aim of this work was the production and characterization of a gel produced in a blue corn flour fermentation process, where different proportions were used of blue corn (Zea mays L.) flour and Czapek Dox culture medium (90 mL of culture medium with 10 g of blue corn flour, 80 mL of culture medium with 20 g of blue corn flour, and 70 mL of culture medium with 30 g of blue corn flour) and were fermented for three different durations (20, 25, and 30 days) with the Colletotrichum gloeosporioides fungus. A characterization of the gel was carried out studying the rheological properties, proximal analysis, toxicological analysis, microscopic structure, and molecular characterization, in addition to a solubility test with three different organic solvents (ethanol, hexane, and ethyl acetate, in addition to water). The results obtained showed in the rheological analysis that the gel could have resistance to high temperatures and a reversible behavior. The gel is soluble in polar solvents (ethanol and water). The main chemical components of the gel are carbohydrates, especially polysaccharides, and it was confirmed by FT-IR spectroscopy that the gel may be composed of pectin.
Genetic Screening—Emerging Issues
Authors: Cornel Martina C. , van der Meij Karuna R. M. , van El Carla G. , Rigter Tessel , Henneman Lidewij
Published: May 2024

In many countries, some form of genetic screening is offered to all or part of the population, either in the form of well-organized screening programs or in a less formalized way. Screening can be offered at different phases of life, such as preconception, prenatal, neonatal and later in life. Screening should only be offered if the advantages outweigh the disadvantages. Technical innovations in testing and treatment are driving changes in the field of prenatal and neonatal screening, where many jurisdictions have organized population-based screening programs. As a result, a greater number and wider range of conditions are being added to the programs, which can benefit couples’ reproductive autonomy (preconception and prenatal screening) and improve early diagnosis to prevent irreversible health damage in children (neonatal screening) and in adults (cancer and cascade screening). While many developments in screening are technology-driven, citizens may also express a demand for innovation in screening, as was the case with non-invasive prenatal testing. Relatively new emerging issues for genetic screening, especially if testing is performed using DNA sequencing, relate to organization, data storage and interpretation, benefit–harm ratio and distributive justice, information provision and follow-up, all connected to acceptability in current healthcare systems.
Evaluation of miR-148a-3p and miR-106a-5p as Biomarkers for Prostate Cancer: Pilot Study
Authors: Coman Roxana , Schitcu Vlad , Budisan Liviuta , Raduly Lajos , Braicu Cornelia , Petrut Bogdan , Coman Ioan , Berindan-Neagoe Ioana , Al Hajjar Nadim
Published: May 2024

MicroRNAs (miRNAs) are a class of small non-coding RNAs that may function as tumor suppressors or oncogenes. Alteration of their expression levels has been linked to a range of human malignancies, including cancer. The objective of this investigation is to assess the relative expression levels of certain miRNAs to distinguish between prostate cancer (PCa) from benign prostatic hyperplasia (BPH). Blood plasma was collected from 66 patients diagnosed with BPH and 58 patients with PCa. Real-time PCR technology was used to evaluate the relative expression among the two groups for miR-106a-5p and miR-148a-3p. The significant downregulation of both miRNAs in plasma from PCa versus BPH patients suggests their potential utility as diagnostic biomarkers for distinguishing between these conditions. The concurrent utilization of these two miRNAs slightly enhanced the sensitivity for discrimination among the two analyzed groups, as shown in ROC curve analysis. Further validation of these miRNAs in larger patient cohorts and across different stages of PCa may strengthen their candidacy as clinically relevant biomarkers for diagnosis and prognosis.
Immunogenetics of Systemic Sclerosis
Authors: Gumkowska-Sroka Olga , Kotyla Kacper , Kotyla Przemysław
Published: May 2024

Systemic sclerosis (SSc) is a rare autoimmune connective tissue disorder characterized by massive fibrosis, vascular damage, and immune imbalance. Advances in rheumatology and immunology over the past two decades have led to a redefinition of systemic sclerosis, shifting from its initial perception as primarily a “hyperfibrotic” state towards a recognition of systemic sclerosis as an immune-mediated disease. Consequently, the search for genetic markers has transitioned from focusing on fibrotic mechanisms to exploring immune regulatory pathways. Immunogenetics, an emerging field at the intersection of immunology, molecular biology, and genetics has provided valuable insights into inherited factors that influence immunity. Data from genetic studies conducted thus far indicate that alterations in genetic messages can significantly impact disease risk and progression. While certain genetic variations may confer protective effects, others may exacerbate disease susceptibility. This paper presents a comprehensive review of the most relevant genetic changes that influence both the risk and course of systemic sclerosis. Special emphasis is placed on factors regulating the immune response, recognizing their pivotal role in the pathogenesis of the disease.
Biogeochemical Response of the Water Column of Concepción Bay, Chile, to a New Regime of Atmospheric and Oceanographic Variability
Authors: Bustos-Espinoza Luis , Torres-Ramírez Patricio , Figueroa Sergio , González Pablo S. , Pavez Marcelo A. , Jerez Rodolfo , Saldías Gonzalo S. , Espinoza Claudio , Galán Alexander
Published: May 2024

Concepción Bay is a socio-economic and ecologically important embayment whose hydrographic variability has been historically regulated by wind-modulated seasonal upwelling events during spring–summer and by freshwater from precipitation and river discharges during fall–winter. This system is subject to several anthropogenic and environmental strains due to the intense port activity and the increasing occurrence of extreme natural events. This study determines a new hydrographic regime and characterizes and analyzes the biogeochemical response of the water column to changes in rainfall and upwelling patterns. Despite the intrusion of nitrate-rich upwelled waters that enhance biological productivity remains more intense during spring–summer, the system remains fertilized year-long due to the occurrence of persistent upwelling pulses during fall–winter. The hydrographic structure presented a two-layer water column that was stratified thermally in spring–summer and primarily by freshwater inputs in fall–winter. Nevertheless, the regular pattern of the rainfall has changed (a decrease in precipitation and an increased frequency of extreme rainfall events), together with recurrent upwelling-favorable wind pulses during the non-upwelling season. This new regime has altered the seasonality of the physicochemical conditions and the structure of the microplanktonic communities, with productive and sanitary implications affecting the biogeochemical status of CB.
Cerebral Blood Flow in Alzheimer’s Disease: A Meta-Analysis on Transcranial Doppler Investigations
Authors: Zuin Marco , De Vito Alessandro , Romagnoli Tommaso , Polastri Michele , Capatti Eleonora , Azzini Cristiano , Brombo Gloria , Zuliani Giovanni
Published: May 2024

Background: Cerebrovascular hemodynamic impairment has been reported in Alzheimer’s disease (AD). We performed a systematic review and meta-analysis to investigate changes in cerebral blood flow (CBF) in AD patients. Methods: Data were obtained by searching MEDLINE and Scopus for all investigations published between 1 January 2011 and 1 November 2021, comparing the cerebrovascular hemodynamic between AD patients and cognately healthy age-matched controls, using transcranial Doppler (TCD) ultrasound. Results: Twelve studies, based on 685 patients [395 with AD and 290 age-matched cognitively healthy controls, with a mean age of 71.5 and 72.1 years, respectively] were included in the analysis. A random effect model revealed that AD patients, in the proximal segments of the middle cerebral artery (MCA), have a significantly lower CBF velocity, compared to controls (MD: −7.80 cm/s, 95%CI: −10.78 to −5.13, p < 0.0001, I2 = 71.0%). Due to a significant Egger’s test (t = 3.12, p = 0.008), a trim-and-fill analysis was performed, confirming the difference (MD: −11.05 cm/s, 95%CI: –12.28 to −9.82, p < 0.0001). Meta-regression analysis demonstrated that the mean CBF at the proximal MCA was directly correlated with arterial hypertension (p = 0.03) and MMSE score (p < 0.001), but inversely correlated with age (p = 0.01). In AD patients, the pulsatility index was significantly higher compared to controls (MD: 0.16, 95%CI: 0.07 to 0.25, p < 0.0001, I2: 84.5%), while the breath-holding index test results were significant lower (MD: −1.72, 95%CI: −2.53 to −0.91, p < 0.001, I2: 85.4%). Conclusions: AD patients have a significant impairment in relation to their cerebrovascular perfusion, suggesting that cerebrovascular hemodynamic deterioration, evaluated using TCD, may be a useful diagnostic tool.