bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapid, High‐Resolution Magnetic Microscopy of Single Magnetic Microbeads
Authors: McCoey Julia M. , de Gille Robert W. , Nasr Babak , Tetienne Jean‐Philippe , Hall Liam T. , Simpson David A. , Hollenberg Lloyd C. L.
Published: March 2019

Magnetic microparticles or “beads” are used in a variety of research applications from cell sorting through to optical force traction microscopy. The magnetic properties of such particles can be tailored for specific applications with the uniformity of individual beads critical to their function. However, the majority of magnetic characterization techniques quantify the magnetic properties from large bead ensembles. Developing new magnetic imaging techniques to evaluate and visualize the magnetic fields from single beads will allow detailed insight into the magnetic uniformity, anisotropy, and alignment of magnetic domains. Here, diamond‐based magnetic microscopy is applied to image and characterize individual magnetic beads with varying magnetic and structural properties: ferromagnetic and superparamagnetic/paramagnetic, shell (coated with magnetic material), and solid (magnetic material dispersed in matrix). The single‐bead magnetic images identify irregularities in the magnetic profiles from individual bead populations. Magnetic simulations account for the varying magnetic profiles and allow to infer the magnetization of individual beads. Additionally, this work shows that the imaging technique can be adapted to achieve illumination‐free tracking of magnetic beads, opening the possibility of tracking cell movements and mechanics in photosensitive contexts.
Non‐Magnetic Injectable Implant for Magnetic Field‐Driven Thermochemotherapy and Dual Stimuli‐Responsive Drug Delivery: Transformable Liquid Metal Hybrid Platform for Cancer Theranostics
Authors: Wang Dan , Xie Wensheng , Gao Qin , Yan Hao , Zhang Junxin , Lu Jingsong , Liaw BorShuang , Guo Zhenhu , Gao Fei , Yin Lan , Zhang Guifeng , Zhao Lingyun
Published: March 2019

Transformable liquid metal (LM)‐based materials have attracted considerable research interest in biomedicine. However, the potential biomedical applications of LMs have not yet been fully explored. Herein, for the first trial, the inductive heating property of gallium–indium eutectic alloy (EGaIn) under alterative magnetic field is systematically investigated. By virtue of its inherent metallic nature, LM possesses excellent magnetic heating property as compared to the conventional magnetite nanoparticles, therefore enabling its unique application as non‐magnetic agents in magnetic hyperthermia. Moreover, the extremely high surface tension of LM could be dramatically lowered by a rather facile PEGylation approach, making LM an ideal carrier for other theranostic cargos. By incorporating doxorubicin (DOX)‐loaded mesoporous silica (DOX‐MS) within PEGylated LM, a magnetic field‐driven transformable LM hybrid platform capable of pH/AFM dual stimuli‐responsive drug release and magnetic thermochemotherapy are successfully fabricated. The potential application for breast cancer treatment is demonstrated. Furthermore, the large X‐ray attenuation ability of LM endows the hybrid with the promising ability for CT imaging. This work explores a new biomedical use of LM and a promising cancer treatment protocol based on LM hybrid for magnetic hyperthermia combined with dual stimuli‐responsive chemotherapy and CT imaging.
Students' recognition of educational demands in the context of a socioscientific issues curriculum
Authors: Lindahl Mats G. , Folkesson Anne‐Mari , Zeidler Dana L.
Published: March 2019

Students' difficulties in interpreting what counts as knowledge have been addressed in past research on science education. The implementation of progressivist pedagogy in terms of more student‐active classroom practice and the introduction of a variety of discourses into the science classroom deepens students' difficulties. The integration of different forms and demands of knowledge and discourses typified by Science‐in‐Context initiatives, such as within the socioscientific framework, exemplifies this development in science education. Here, the diffuse boundaries between school subjects and other silos of knowledge lead to considerable difficulties for students to interpret what is expected from them. Such contexts having diffuse boundaries between, for example, subject discourses and other forms of knowledge, have been describes as contexts with weak classification. The present study aims to explore students' interpretation of what knowledge or meaning they are requested to produce in contexts with weak classification, here exemplified within an SSI‐task. We use Bernstein's concepts of recognition rules and classification to analyze how 15‐ to 16‐year‐old students develop their discussions in groups of 4–6 students. This study reports how students' recognition of the educational demands enabled integration of different discourses in their discussion, and that the use of both universalistic and particularistic meanings can produce new understandings. Students who had not acquired recognition rules were found to keep discourses apart, expressed either as rejection of the relevance of the task, answering questions as in a traditional school task, or just exchange of personal opinions. Furthermore, they included discourses irrelevant to the issue. An important outcome of the study was that socioscientific thinking was hampered when students kept universalistic and particularistic meanings apart. This hampering results from the inhibition of dynamic exploration during SSI discussions. The results provide new insights with relevance for teachers' guiding students toward a fruitful SSI‐discourse.
Flux linkage optimization for direct torque control of switched reluctance motor based on model predictive control
Authors: Shang Chaoyi , Xu Aide , Huang Lepeng , Chen Jiagui
Published: March 2019

In this paper, to solve the problem of large torque ripple in a switched reluctance motor (SRM), a new control strategy based on model predictive flux control is proposed in the direct torque control (DTC) algorithm. First, the flux linkage value in the next period is calculated from the discrete time model of the SRM. Second, to reduce the computation burden and increase the dynamic response, a torque hysteresis controller is embedded in the system to select three candidate voltage vectors for the cost function. Finally, based on flux linkage minimization, the best voltage vector is selected and applied to the system. The proposed direct torque and predictive flux control (DTPFC) method perfectly suits the nonlinear magnetic characteristics of SRM, and shows better steady‐state and dynamic‐state performances compared to DTC. Furthermore, since DTPFC has only one variable in the cost function, the huge task of obtaining the best weighting factor is eliminated. Simulation and experiments are carried out on a three‐phase 12/8 SRM with the proposed control algorithm and DTC. The results demonstrate the validity of the new control approach. © 2019 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
Crosslinked glutinous rice starch filled polyvinyl alcohol films
Authors: Yeng Chan Ming , Chun Koay Seong
Published: March 2019

The influence of glutinous rice starch (GRS) content and sodium hexametaphosphate (SHMP) in polyvinyl alcohol (PVOH) films were studied. The increase of GRS content (0–40 wt%) reduced the tensile strength (from 14.3 to 4.3 MPa) and elongation at break (from 183 to 52.5) of PVOH/GRS films. Nevertheless, the modulus of elasticity of PVOH/GRS films increased with GRS content, from 20.3 to 132.83 MPa. SHMP was used as a crosslinking agent, which improved more than 30% of tensile strength and modulus of elasticity of PVOH/GRS films. However, the elongation at break reduced after crosslinking process of the films. The crosslinked film showed better interaction between GRS and PVOH, as demonstrated by scanning electron microscopy. Conversely, the crosslinked films exhibited a lower swelling degree, but a higher gel content compared to uncrosslinked films. J. VINYL ADDIT. TECHNOL., 25:359–365, 2019. © 2019 Society of Plastics Engineers
Data assimilation impact of in situ and remote sensing meteorological observations on wind power forecasts during the first Wind Forecast Improvement Project (WFIP)
Authors: Wilczak James M. , Olson Joseph B. , Djalalova Irina , Bianco Laura , Berg Larry K. , Shaw William J. , Coulter Richard L. , Eckman Richard M. , Freedman Jeff , Finley Catherine , Cline Joel
Published: March 2019

During the first Wind Forecast Improvement Project (WFIP), new meteorological observations were collected from a large suite of instruments, including wind velocities measured on networks of tall towers provided by wind industry partners, wind speeds measured by cup anemometers mounted on the nacelles of wind turbines, and wind profiles by networks of Doppler sodars and radar wind profilers. Previous data denial studies found a significant improvement of up to 6% root mean squared error (RMSE) reduction for short‐term wind power forecasts due to the assimilation of all of these observations into the National Oceanic and Atmospheric Administration (NOAA) Rapid Refresh (RAP) forecast model using a 3D variational data assimilation scheme. As a follow‐on study, we now investigate the impacts of assimilating into the RAP model either the additional remote sensing observations (sodars and radar wind profilers) alone or assimilating the industry‐provided in situ observations (tall towers and nacelle anemometers) alone, in addition to routinely available standard meteorological data sets. The more numerous tall tower/nacelle observations provide a relatively large improvement through the first 3 to 4 hours of the forecasts, which diminishes to a negligible impact by forecast hour 6. In comparison, the sparser vertical profiling sodars/radars provide an initially smaller impact that decays at a much slower rate, with a positive impact present through the first 12 hours of the forecast. Large positive assimilation impacts for both sets of instruments are found during daytime hours, while small or even negative impacts are found during nighttime hours.
Degradation of chloramphenicol using Ti‐Sb/attapulgite ceramsite particle electrodes
Authors: Sun Yongjun , Chen Aowen , Zhu Sichen , Sun Wenquan , Shah Kinjal J. , Zheng Huaili
Published: March 2019

Ti‐Sb/attapulgite ceramsite particle electrodes were prepared for the efficient degradation of chloramphenicol (CAP) in wastewater. To observe the surface morphology and structural characteristics of the Ti‐Sb/attapulgite ceramsite particle electrodes, Fourier transform infrared spectroscopy, X‐ray fluorescence, scanning electron microscopy, and X‐ray diffraction were used for characterization. Parameters affecting the degradation efficiency and the energy consumption of the Ti‐Sb/attapulgite ceramsite particle electrodes, such as current density, electrode distance, initial pH, conductivity, air flow, and packing ratio, were examined. Results showed that the optimal conditions were 20 mA/cm2 current intensity, 3 cm electrode distance, 5,000 μS/cm conductivity, 2.0 L/min air flow, 50.0% packing ratio, and initial pH 1. The removal efficiency of chloramphenicol was 73.7% under the optimal conditions, and the energy consumption was 191.3 (kW h)/kg CAP.

Practitioner points

Attapulgite ceramsite with good physical performance was prepared.

The removal efficiencies for CAP in water by Ti‐Sb/attapulgite ceramsite particle electrodes were studied.

CAP was effectively removed by 3‐D electrode system.

CAP removal was significantly influenced by operational parameters.

Three‐dimensional electrode system shows good degradation ability.
Conservation of asymptotic charges from past to future null infinity: supermomentum in general relativity
Authors: Prabhu Kartik
Published: March 2019

Abstract

We show that the BMS-supertranslations and their associated supermomenta on past null infinity can be related to those on future null infinity, proving the conjecture of Strominger for a class of spacetimes which are asymptotically-flat in the sense of Ashtekar and Hansen. Using a cylindrical 3-manifold of both null and spatial directions of approach towards spatial infinity, we impose appropriate regularity conditions on the Weyl tensor near spatial infinity along null directions. The asymptotic Einstein equations on this 3-manifold and the regularity conditions imply that the relevant Weyl tensor components on past null infinity are antipodally matched to those on future null infinity. The subalgebra of totally fluxless supertranslations near spatial infinity provides a natural isomorphism between the BMS-supertranslations on past and future null infinity. This proves that the flux of the supermomenta is conserved from past to future null infinity in a classical gravitational scattering process provided additional suitable conditions are satisfied at the timelike infinities.
Supersymmetry breaking in a large N gauge theory with gravity dual
Authors: Honda Masazumi , Nosaka Tomoki , Shimizu Kazuma , Terashima Seiji
Published: March 2019

Abstract

We study phase structure of a three dimensional

$$ \mathcal{N} $$

N

= 6 superconformal theory deformed by mass parameters called mass-deformed ABJM theory, which has the gauge group U(N) × U(N) with Chern-Simons levels (k, −k) and may have a gravity dual. We discuss that the mass deformed ABJM theory on S
3 breaks supersymmetry in a large-N limit if the mass is larger than a critical value. To see some evidence for this conjecture, we compute the partition function exactly, and numerically by using the Monte Carlo Simulation for some finite values of N. We discover that the partition function has zeroes as a function of the mass deformation parameters if N ≥ k, which supports the large-N supersymmetry breaking. We also find a solution to the large-N saddle point equations, where the free energy is consistent with the finite N result.
Design-features of bubble-prone experimental asset markets with a constant FV
Authors: Huber Christoph , Bindra Parampreet C. , Kleinlercher Daniel
Published: March 2019

Experimental asset markets with a constant fundamental value (<mml:math xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mnf="http://cambridge.org/core/manifest" xmlns:cup="http://contentservices.cambridge.org" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:m="http://cambridge.org/core/metadata" xmlns:core="http://cambridge.org/core" xmlns:c="http://cambridge.org/core/content">FV) have grown in importance in recent years. A methodological examination of the robustness of experimental results in such a setting which has been shown to produce bubbles, however, is lacking. In a laboratory experiment with 280 subjects, we investigate whether specific design features are sufficient to influence experimental results. In detail, we (1) vary the visual representation of the price chart, and (2) provide subjects with full information about the FV process. We find overvaluation and bubble formation to be reduced when trading prices are displayed at the upper end of the price chart. Surprisingly, we do not find any effects when subjects have full information about the FV process.
Erectile Dysfunction Medication Prescription: STI and Risk Behavior in Men with HIV
Authors: Heudebert Jose Pablo , Tamhane Ashutosh , Burkholder Greer A. , Dionne-Odom Jodie
Published: March 2019

Introduction

Erectile dysfunction (ED) is a common diagnosis in up to 50% of men with HIV and prescription of erectile dysfunction medication (EDM) has been variably associated with increased risk behaviors and acquisition of sexually transmitted infections (STIs).

Aim

We measured the association of EDM prescription with bacterial STI testing, STI infection and sexual behavior among men engaged in HIV care.

Methods

A retrospective cohort study was conducted among HIV-infected men in care at an urban HIV clinic in Birmingham, Alabama between 2008 and 2016. Paired data analysis was used to compare STI testing and behavioral outcomes during the 12-month period before and after EDM prescription.

Main Outcome Measures

Our study outcomes were STI testing and infection rates for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (GC) and incident syphilis as well as risk behaviors before and after EDM prescription.

Results

Of 2924 HIV-infected men engaged in care, 589 (20%) initiated EDM with a new prescription from a clinic provider during the study period. During the year after EDM prescription, all STI testing rates decreased: CT (OR = 0.76; 95% CI: 0.58 - 1.01; P = .06), GC (OR = 0.76; 95% CI: 0.58 - 1.01; P = .06), and syphilis (OR = 0.28; 95% CI: 0.20 - 0.38; P < .001). A total of 43 STIs were detected in this study (10 CT, 8 GC, and 25 syphilis) and 42/43 occurred among men who have sex with men (MSM). Sexual activity rates were high before and after EDM (87.6% vs 82.9%; P = .08), and consistent condom use was rare (6.6% in both time periods). After EDM prescription, the median number of sexual partners in the past 6 months decreased from 2 to 1 among MSM and was stable at 1 among men who have sex with women.

Clinical Implication

Management of ED in HIV clinic provides an excellent opportunity to discuss risk reduction, safer sex practices, and the importance of routine STI screening to prevent HIV/STI transmission.

Strength & Limitations

This study provides insight into a common but understudied clinical scenario-ED in men with HIV-in an urban clinic population that is representative of the Southeastern United States. Adherence for ED medication was not assessed and STI risk behaviors were self-reported.

Conclusion

EDM prescription did not lead to any detectable change in risk behavior in this setting but bacterial STI was common among MSM who were tested.
Consent in the Exam Room
Authors: Keller Jennifer Mendillo
Published: March 2019

Introduction

Explicitly obtaining consent from a patient prior to starting a sensitive physical exam is essential to improve their experience with intimate examinations such as the genitourinary exam.

Aim

The purpose of this article is to raise awareness among providers about how and why to obtain consent for intimate examinations.

Methods

The current literature on consent was reviewed and the opinion piece was created using current events and literature.

Results

Providers have a responsibility to consent patients for a sensitive exam.

Conclusion

Explicitly obtaining consent from a patient priro to starting a sensitive physical exam such as a genitourinary exam is essential to improve the patient experience with such exams.
Changes in Female Sexual Function After Vaginal Mesh Repair Versus Native Tissue Repair for Pelvic Organ Prolapse: A Meta-Analysis of Randomized Controlled Trials
Authors: Liao Sao-Chun , Huang Wen-Chu , Su Tsung-Hsien , Lau Hui-Hsuan
Published: March 2019

Aim

To evaluate changes in female sexual function after transvaginal mesh (TVM) repair versus native tissue repair for pelvic organ prolapse.

Methods

Eligible studies, published through November 2017, were retrieved through searches of ClinicalTrials.gov, MEDLINE, Embase, and Cochrane Review databases and associated bibliographies. We included randomized control trials of transvaginal prolapse surgery with either mesh repair or native tissue repair regarding the outcomes of sexual function, de novo and postoperative dyspareunia with a minimum of 3 months of follow-up.

Results

Seventeen trials including 2,976 patients (1,488 with TVM repair and 1,488 with native tissue repair) were identified. There was no significant difference in postoperative dyspareunia after TVM repair versus native tissue repair (risk ratio [RR] = 1.07; 95% confidence interval [CI] = 0.76–1.50). Likewise, there was no significant difference in de novo dyspareunia after TVM repair versus native tissue repair (RR = 0.91; 95% CI = 0.52–1.61). There was also no significant difference in the short form Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire score after TVM mesh repair versus native tissue repair (mean difference = 0.26; 95% CI = -1.34 to 1.85).

Conclusion

Sexual function and de novo and postoperative dyspareunia were similar between the patients who underwent TVM repair and those who underwent native tissue repair.
3010 Effects of non-invasive brain stimulation on speech fluency and brain activity in adults who stutter: a randomized controlled clinical trial
Authors: O’Dell Garnett Emily , Changv Soo-Eun , Hampstead Benjamin , Chow Ho Ming
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: The goal of this study is to measure speech fluency and brain activity before and after 5 days of behavioral speech fluency training alone (sham group) or speech training plus stimulation (active group). A 1-month follow up will also be completed. The first primary outcome measure is changes in brain activation in speech motor control/timing network. The second primary outcome measure is changes in percentage of stuttered syllables during speech sample (speech fluency). The secondary outcome measure is changes from baseline on the Overall Assessment of Speakers Experience of Stuttering (OASES), a detailed subject rating of how stuttering affects their lives. METHODS/STUDY POPULATION: This study is a between subjects, counterbalanced, sham-controlled, double-blind design. Participants will be 40 adults who stutter who will be randomized (using minimization) into either the active or sham stimulation group, with all other study procedures being the same in both groups. Participants will completed 2 days of baseline testing, 5 consecutive days of brain stimulation during speech training, 2 days of post-testing, and a 1-month follow up. All outcome measures will be completed immediately before and after the 5 days of brain stimulation, as well as at follow-up. as of submission, 10 subjects have completed the study. Data collection is ongoing. RESULTS/ANTICIPATED RESULTS: Expected results. Questions this study aims to answer: 1) Does a more intensive training period lead to decreased stuttering? We expect that both groups will show improvements in speech fluency immediately after training. We expect that those in the active group will continue to exhibit improved speech fluency at 1 month follow up. 2) Does a more intensive training period lead to changes in brain activity? We expect that both groups will exhibit increased activity in auditory/motor regions immediately after training. We expect that the active group will continue to exhibit an increase in activity in these regions at 1 month follow up. DISCUSSION/SIGNIFICANCE OF IMPACT: This is the first RCT study involving brain stimulation in adults who stutter. We expect to provide preliminary evidence for the effectiveness of tDCS as an augmentative agent for increased speech fluency in adults who stutter during a brief, intensive training paradigm. We also expect to be able to provide information on the effects of tDCS on brain activity in speech and auditory-motor regions of the brain. The findings will add to the growing body of literature suggesting that developmental stuttering is a neurodevelopmental disorder with roots in timing and rhythmic aspects of speech motor control and auditory-motor integration.
3092 Measuring Fluid Compartments Before and After Rapid Saline Infusion
Authors: Kelly Kevin Lawrence , Carlson Alex R. , Cierzan Bradley B. , Isautier Jennifer , Miller Wayne L. , Johnson Bruce D.
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: To evaluate the ability of various techniques to track changes in body fluid volumes before and after a rapid infusion of saline. METHODS/STUDY POPULATION: Eight healthy participants (5M; 3F) completed baseline measurements of 1) total body water using ethanol dilution and bioelectrical impedance analysis (BIA) and 2) blood volume, plasma volume and red blood cell (RBC) volume using carbon monoxide rebreathe technique and I-131 albumin dilution. Subsequently, 30mL saline/kg body weight was administered intravenously over 20 minutes after which BIA and ethanol dilution were repeated. RESULTS/ANTICIPATED RESULTS: On average, 2.29±0.35 L saline was infused with an average increase in net fluid input-output (I/O) of 1.56±0.29 L. BIA underestimated measured I/O by −3.4±7.9%, while ethanol dilution did not demonstrate a measurable change in total body water. Carbon monoxide rebreathe differed from I-131 albumin dilution measurements of blood, plasma and RBC volumes by +0.6±2.8%, −5.4±3.6%, and +11.0±4.7%, respectively. DISCUSSION/SIGNIFICANCE OF IMPACT: BIA is capable of tracking modest changes in total body water. Carbon monoxide rebreathe appears to be a viable alternative for the I-131 albumin dilution technique to determine blood volume. Together, these two techniques may be useful in monitoring fluid status in patients with impaired fluid regulation.
3168 Clinical and Translational Mentoring Team (CTMT): Effective Strategy for the Development of Students – undergraduate (US) and graduate (GS) – and Faculty (F) of Health Sciences Programs (HSPs) in Clinical and Translational Research (CTR) in Puerto Rico
Authors: Irizarry-Ramírez Margarita , González-Méndez María E. , Moscoso-Álvarez José R. , García Rubén García
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: The Title V Cooperative Project between the University of Puerto Rico- Medical Sciences Campus (UPR-MSC) and Universidad Central del Caribe (UCC) has trained US, GS and F (participants) of HSPs to engage them in CTR. METHODS/STUDY POPULATION: First stage of the training sessions (TS) dealt with the theory of CTR. After TS and responding to their research interests, as answered in a questionnaire, the participants formed a CTMT, under the mentorship of a well-established CT researcher. This, as a prelude to their hands-on experiences in Intensive Development and Experiences in Advancement of Research and Increased Opportunities (IDEARIO), for which a research proposal is needed. RESULTS/ANTICIPATED RESULTS: Five (5) CTMTs were formed in different research areas – cardio, neuro, liver, renal, Zika–, as submitted in their research concept papers.Eight (8) CT researchers are currently mentoring 2 US, 7 GS and 6 F of HSPs through the CTMTs. They have submitted a research proposal, as a bridge between the theory in the TS and the practice in IDEARIO. Five (5) proposals were received and 2 of them approved, while the other 3 are in the evaluation process. We will present the composition, research topics, development of research and the feedback of participants in IDEARIO and CTMTs. DISCUSSION/SIGNIFICANCE OF IMPACT: The CTMTs and their respective proposals are effective strategies for the mentoring of US, GS and F in CTR.
3249 Defining the Extracellular Vesicle Content of Interstitial Fluid for Blood-Free Diagnostics; Extraction Methods and Initial Characterization
Authors: Robert Taylor Justin Baca , Gadam Srinivasa Rao , Perez Lauren
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: Recent advances in microneedle technology have enabled practical, in vivo dermal interstitial fluid (ISF) sampling. These minimally-invasive techniques allow for collection of ISF without damage to adjacent tissues and do not rely on blister formation. Initial reports of extracellular vesicle (EV) isolation from dermal ISF and paired blood samples suggest that EVs may be more abundant in ISF. Analysis of ISF-derived EVs may allow for more detailed study of intercellular communication at the tissue level, particularly in acute inflammatory conditions. The objective of this study is to describe the isolation and initial characterization of interstitial fluid-derived exosomes. METHODS/STUDY POPULATION: We apply electron microscopy, nanoparticle tracking analysis (NTA), immuochemical, and sequencing methods to describe and distinguish the EV content of interstitial fluid. We include apparently healthy adult human subjects with no active skin disease. We also study immunocompetent, CD-hairless rats to demonstrate the generalizability of the methods. RESULTS/ANTICIPATED RESULTS: We successfully isolated EVs from human and rat interstitial fluid using commercially available precipitation methods. The EVs were initially characterized using UV/Vis spectroscopy, electron microscopy, and NTA. While the study is ongoing, initial results suggest that the concentration and size distribution of EVs differs significantly between blood fractions and ISF. Further immunochemical and sequencing characterization is ongoing. DISCUSSION/SIGNIFICANCE OF IMPACT: We present here the initial characterization of EVs isolated from dermal interstitial fluid. This appears to be the first report of EV characterization using ISF collection methods that do not perturb adjacent tissues (such as with blister or microdialysis methods). The present study lays a foundation for further examination of ISF-derived EVs in acute inflammatory disease such as cellulitis or infectious neuritis. This may enable minimally invasive diagnostics and new research tools to understand intercellular communication in living organisms with increased spatial and temporal resolution.
3113 Formative Evaluation of a Safety Baby Shower Intervention for Rural African American Parents and Community Advisors
Authors: Nabaweesi Rosemary , Aitken Mary , Mullins Samantha H. , Bryant-Moore Keneshia , Curran Geoffrey M. , Harris Zenobia
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: To explore rural African American parents’ and their community advisors’ perspectives on the Safety Baby Shower’s acceptability, feasibility, and adaptability. METHODS/STUDY POPULATION: Collaborating with a local community organization, we explored community advisors’ and expectant women’s SBS experiences to understand intervention delivery and adoption in a rural underserved community (RUC). The Consolidated Framework for Implementation Research guided our data collection and analysis using focus groups and key informant interviews. We used directed content analysis to generate themes and sub codes. RESULTS/ANTICIPATED RESULTS: Five focus groups (21 participants) and one key informant interview were conducted. Identified barriers that hinder feasibility and acceptability included resources, time/ flexibility, intervention location, cultural norms and beliefs, and the lack of a birthing hospital in the county. “Baby proofing”, “reinforcement products” and “teaching sleep safety on the same day as infant clinical appointment” are expectant mothers’ exemplars for what comes to their minds when asked to think about safety baby showers. To improve feasibility, both community advisors and expectant mothers suggested adaptations ranging from decentralizing or rotating intervention location, using different delivery sites such as churches, scheduling intervention outside business hours, to incorporating intervention into school health fairs and barbeque events. Social media emerged as a facilitator, and integrating safe sleep education into personal baby showers emerged as an implementation strategy. DISCUSSION/SIGNIFICANCE OF IMPACT: The community advisors and expectant mothers identified a wide spectrum of potential adaptations that have potential to improve safe sleep knowledge and practices. In the next study phase, identified themes will inform intervention adaptation and suggested implementation strategies will support uptake of the adapted SBS. Identifying transformative implementation strategies and conducting a community-informed SBS adaptation using a collective decision-making process between intervention experts and local community partners will support improved safety baby shower delivery, adoption and sustainability in RUCs.
3392 Tapping into Community Insight and Lived Experience to Inform, Guide and Direct Translational Science Initiatives
Authors: McNeeley Miles , Kubicek Katrina , Baezconde-Garbanati Lourdes , Lincoln Karen D. , Kipke Michele
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: This study aims to describe adaptability in methods used to apply community input to programming within the field of translational science. The outcomes of community informed programming include opportunities for innovative projects and approaches, and better responsiveness to community needs. It is anticipated that this will result in greater community involvement in research, moving towards greater health equity. METHODS/STUDY POPULATION: The SC CTSI is situated in urban Los Angeles, one of the most diverse communities in the world. Eight SC CTSI Community Engagement Core initiatives that employ community partnership are illustrated. The activities include social marketing campaigns for cervical cancer prevention; use of community-embedded research ambassadors to increase scientific literacy in Latino and Black/African-American communities; use of innovative technologies to educate pediatric patients and families about clinical research; working with the entertainment industry to promote clinical research in popular television shows; a community advisory board that is tailored and embedded in each CSTA core group; a community based research dissemination program; an ad-hoc community advisory group assembled to adapt a research 101 curriculum for Black/African-American communities; and a series of listening sessions conducted throughout Los Angeles. RESULTS/ANTICIPATED RESULTS: Integration of community voices provide direction for future planning, programming and execution of all referenced initiatives. Ultimately, the goal for these discussions with community members is to develop innovative approaches to CTSA programming. DISCUSSION/SIGNIFICANCE OF IMPACT: Racial and ethnic minorities continue to experience underrepresentation in clinical research trials. CTSAs have been tasked with addressing barriers that have historically led to disparities in research participation, and by extension, the effectiveness of medical interventions in diverse populations. Community input is an invaluable source for knowledge and innovative ideas in how to increase involvement in various aspects of the research process, including dissemination, recruitment and enrollment in clinical trials. CTSAs have increasingly augmented Community Engagement programs within their respective cores to address population disparities. The approaches used to engage communities require an element of fluidity and flexibility, and a reliance on the input of community members, in order to maintain relevant and desired community engagement practices.
3543 Translating the complex medical jargon of opioid use disorder and medication assisted treatment into locally relevant messages in rural Colorado using Boot Camp Translation
Authors: Fisher Mary , Nease Donald E. , Zittleman Linda , Westfall Jack , Ancona Jennifer
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: Opioid use disorder (OUD) is a national epidemic and identified as a top priority by the practices and communities in rural Colorado. Until recently, few resources existed to address OUD in rural communities. In addition to training primary care and behavioral health practice teams in medication assisted treatment (MAT), Implementing Technology and Medication Assisted Treatment and Team Training and in Rural Colorado (IT MATTTRs Colorado) engaged local community members to alter the community conversation around OUD and treatment. For IT MATTTRs, the High Plains Research Network and the Colorado Research Network engaged community members in a 8-10 month process known as Boot Camp Translations (BCT) to translate medical information and jargon around OUD and MAT into concepts, messages, and materials that are meaningful and actionable to community members. The resulting community interventions are reported here. METHODS/STUDY POPULATION: IT MATTTRs conducted separate BCTs in Eastern Colorado and the south central San Luis Valley. Community partners included non-health professionals with diverse backgrounds, public health and primary care professionals, law enforcement, and others. The BCT process includes a comprehensive education on OUD and MAT and facilitated meetings and calls to develop messages and dissemination strategies. Each BCT lasted around 8-10 months. RESULTS/ANTICIPATED RESULTS: The BCT process elicited unique contextual ideas and constructs for messages, materials, and dissemination strategies. Themes common to both BCTs include the prevalence of OUD and that help is available in the local primary care office. Community-tailored messages are distributed through posters and flyer inserts, drink coasters, newspaper articles, letters to local judges, restaurant placemats, and websites. Examples of the materials and messages will be presented. DISCUSSION/SIGNIFICANCE OF IMPACT: Local community members are eager to help address the OUD crisis. Built on community-based participatory research principles, BCT can be used to translate complex information and guidelines around OUD and MAT into messages and materials that reflect local culture and community needs.
3487 Trust in Research Among Older Adults
Authors: Striley Catherine Woodstock , Lloyd Shawnta , Varma Deepthi , Vaddiparti Krishna , Cottler Linda B.
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: Adults, 60 years of age and older, are in high demand for enrollment in many types of health research. Here we aimed to examine baseline, 60-day and 120-day follow-up trust in research and researchers of Floridians 60 years of age and older engaged in University of Florida’s HealthStreet community engagement initiative. METHODS/STUDY POPULATION: HealthStreet Community Health Workers (CHWs) assess health needs and trust in research of community members and screen for dementia, before providing medical and social services referrals and linkages to opportunities to participate in relevant health research at UF. In addition, participants are followed up at 60 and 120 days. RESULTS/ANTICIPATED RESULTS: Among the 2,193 older adults assessed by CHWs, 62.6% were female, 46.8% were African American, and 6.1% Hispanic/Latino. At baseline, 28.3% reported ever being in a research study; 7.7% reported not being interested in participating in research. Trust in research and researchers was high at baseline [scored from 1 to 10 where 10 was high; mean of 7.4 each for trust in research (SD=2.0) and trust in researchers (SD=2.1)] and high at both follow-ups [60 days 7.8 (SD=2.1) and 7.7 (SD=2) for trust in research and researchers respectively; 120 days 8.0 for both (SD=1.9 and 1.8 respectively)]. DISCUSSION/SIGNIFICANCE OF IMPACT: Individuals who are 60 and older have high trust in research and researchers when approached and high interest in research. Their trust continues through work with HealthStreet CHWs. Community engagement is an important part of the pipeline for recruitment of older adults for research.
3067 Biomechanical analysis of acute versus chronic aortic dissection flaps
Authors: Lou Xiaoying , Sun Wei , Sulejmani Fatiesa , Liu Minliang , Chen Edward , Leshnower Bradley
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: Thoracic endovascular aortic repair (TEVAR) is more effective in remodeling the dissected aorta in acute versus chronic type B aortic dissection (TBAD). It has been hypothesized that this is due to differences in dissection flap biomechanical and structural properties but has not been confirmed in explanted human aortic tissue. We aimed to characterize and compare differences in tissue biomechanics and microstructure between acute and chronic dissection flaps that may underlie these findings. METHODS/STUDY POPULATION: Dissection flaps were obtained at time of operative repair for patients presenting for open aortic replacement to treat acute type A (ACUTE, n=7) or chronic type B (CHRONIC, n=7) aortic dissection. Given that the current treatment modality for acute complicated TBAD is TEVAR, it was not feasible to acquire acute TBAD flaps for analysis. Tissues were cryopreserved and subjected to biaxial tensile testing in the circumferential and longitudinal directions. Stiffness was quantified by the tangent modulus (TM) in the low and high linear regions of the compiled equibiaxial response curves for each cohort. Extensibility was defined as the intersection of the fitted line from the high linear region with the x-axis, and the degree of anisotropy (DA) was defined as the mean absolute percentage error of the strains in both directions. Flap architecture and collagen fiber organization were also compared between groups using two-photon microscopy. RESULTS/ANTICIPATED RESULTS: Average age of dissection flaps were 3.4±3.4 days in ACUTE and 1,868.7±1,354.0 days in CHRONIC (p=0.011). There were no differences in age, co-morbidities, maximum aortic diameter, and aortic wall thickness. ACUTE exhibited an anisotropic stress-strain response with increased extensibility longitudinally than circumferentially (0.18 vs. 0.09, p=0.022, DA=0.67) while CHRONIC demonstrated an isotropic response with similar extensibility in either direction (0.11 vs. 0.12, p=0.606, DA=0.26). CHRONIC and ACUTE had comparable stiffness in the circumferential direction (TMlow 439.92 vs. 541.08, p=0.729, and TMhigh 1585.19 kPa vs. 1869.35 kPa, p=0.817). In the longitudinal direction, CHRONIC was significantly stiffer than ACUTE (TMhigh 8347.61 kPa vs. 1201.34 kPa, p=0.049) (FIGURE). Microscopy corroborated these findings with greater collagen fiber organization circumferentially than longitudinally in ACUTE and increasing fibrosis, collagen predominance, and straightening of collagen fibers in CHRONIC. DISCUSSION/SIGNIFICANCE OF IMPACT: Compared to ACUTE, CHRONIC exhibited loss of anisotropy with increased tissue stiffness in the longitudinal direction. Increased dissection flap fibrosis and decreased compliance may explain the worse outcomes for aortic remodeling after TEVAR in chronic TBAD. This study offers biomechanical support for early TEVAR in the acute phase of uncomplicated TBAD.
3540 Effects of Local Interleukin-6 on Mitochondrial Physiology in Skeletal Muscle
Authors: Abid Hinnah , Hart Corey , Lanza Ian
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: In the context of skeletal muscle, IL-6 plays a major role in muscle quality. The goal of this project was to study the influence of systemic IL-6 on skeletal muscle mitochondrial physiology, most notably mitochondrial function (respiration and ROS production) and mitochondrial content. METHODS/STUDY POPULATION: To determine the influence of interleukin-6 (IL-6) on skeletal muscle mitochondria, high-resolution respirometry was performed to simultaneously measure oxygen consumption (JO2) and ROS production in differentiated myotubes incubated with increasing IL-6 (0, 10, 50, 100 ng/mL) for 18 hours in serum free conditions. To evaluate the impact of IL-6 on mitochondrial content we performed western blots on cell lysates from treated cells, measuring proteins of the mitochondrial electron transport chain (ETC) using a cocktail antibody and PGC-1α/PGC-1ß for mitochondrial biogenesis. To determine the role of mitochondrial ROS production on JO2 and mitochondrial content, we co-treated differentiated myotubes for 18 hours with 50 and 100ng/mL IL-6 and the mitochondrial specific antioxidant, MitoQ and performed respirometry for mitochondrial functional measurements and western blots for mitochondrial content.Statistical significance was evaluated by using a 2-tailed Student’s t-test and two-way ANOVA. Post hoc all-group analyses were conducted to determine which groups were different when the model was significant. RESULTS/ANTICIPATED RESULTS: Mitochondrial functional measurements show increased JO2 and increased ROS production in an IL-6 dose-dependent manner. Targeting mitochondrial ROS production with 0.5µm MitoQ attenuated IL-6 induced increases in JO2 and ROS production. Complexes I and II (CI, CII) of the ETC increased significantly in an IL-6 dose-wise fashion, and co-treatment with MitoQ normalized increases at 100ng/mL Il-6. 100ng/mL IL-6 significantly increased protein expression of PGC-1α and PGC-1ß. Co-treatment with MitoQ normalized IL-6 induced increase in PGC-1α. DISCUSSION/SIGNIFICANCE OF IMPACT: Our data suggest that when treated chronically at a high dose, IL-6 increases mitochondrial respiration, ROS production, and content. Targeting mitochondrial ROS production normalizes these mitochondrial adaptations. The present study provides new insights into mitochondrial physiology in the context of inflammation. Therapeutically targeting mitochondrial ROS production may impact skeletal muscle quality in certain populations.
3432 Stanford MedTech: An Innovative CTSA-Supported Pilot Program
Authors: Dunn Ashley , Lucian Linda , Saul Gordon , Yock Paul , Cullen Mark
Published: March 2019

OBJECTIVES/SPECIFIC AIMS: Helping researchers assess and effectively translate innovations into healthcare improvements is a complex process (Terry et. al., 2013). The Clinical Translational Science Awards (CTSA)—supported by the National Institute of Health (NIH) under the auspices of the National Center for Advancing Translational Sciences (NCATS)— provide the resources and support needed to strengthen our nation’s clinical and translational research (CTR) enterprise. In 2008, Stanford University was awarded a CTSA from the NIH, establishing Spectrum (the Stanford Center for Clinical and Translational Research and Education). Under the Spectrum umbrella, the Byers Center for Biodesign manages the MedTech Pilot Program with the goal of translating discoveries into novel health technologies that address important unmet health needs. The MedTech Pilot Program is an innovative funding mechanism that seeks to (1) stimulate clinical translational research, (2) help promising projects bridge the gap between the bench and the patients’ bedside, and (3) encourage collaborative, transdisciplinary work. Specifically, the Pilot Program offers up to $50,000 to support projects involving medical devices and mobile technologies used for (1) therapeutic applications and (2) device-based patient-specific (or POC) diagnostic applications. This analysis of the MedTech Pilot Program will: 1) describe the Program’s structure and process; 2) highlight the intensive, hands-on mentorship and practical guidance awardees receive that enables them to more efficiently and effectively advance their projects toward patient care; and 3) characterize the progress of the 36 funded projects. METHODS/STUDY POPULATION: Key elements of the Pilot Program’s infrastructure and mentoring processes as they relate to project outcomes were identified. Additionally, outcomes data were collected from two sources: (1) annual survey of Pilot Awardees and (2) publicly available information relevant to the pilot projects. RESULTS/ANTICIPATED RESULTS: The Pilot Program’s framework and infrastructure has supported a diverse group of transdisciplinary projects. These projects were evaluated using both traditional and non-traditional metrics (e.g., patents, startups, publications). The initial investment of $1.5 million to fund 36 projects has led to over $88 million dollars in additional funding. Additionally, taking full advantage of the expertise in Silicon Valley, strong mentorship has helped advance projects along the clinical and translational path. DISCUSSION/SIGNIFICANCE OF IMPACT: The Pilot Program has benefited Stanford innovators and researchers by providing seed funding to help promising projects bridge the gap between the bench and the bedside. The intensive, hands-on mentorship, early pilot funding, and practical guidance pilot awardees receive effectively help translate their technologies into patient care.