bluuebunny/crossref_metadata_2025 huggingface dataset:
Reliability and Validity of the Turkish Version of the Gross Motor Function Measurement (GMFM-88&66) in Children with Cerebral Palsy
Authors: Ataç Tuğçe , Özal Cemil , Kerem Günel Mintaze
Published: September 2024

Background: The gross motor function measurement is considered as the gold standard for the motor assessment of children with cerebral palsy. The aim was to carry out the cross-cultural adaptation and investigate psychometric properties. Methods: A total of 150 children with cerebral palsy aged 2–16 (mean 8.82 ± 3.78 years; 54.7% male) included. The Gross Motor Function Measurement was adapted into Turkish. Two physiotherapies independently administered the gross motor function measurement. Internal consistency and intra/inter-rater reliability were assessed using Cronbach’s alpha, intraclass-correlation-coefficient. Standard-error-of-measurement, minimal-detectible-change calculated. The Bland–Altman method was applied to estimate the measurement bias in reliability analysis. Construct validity assessed with Spearman’s correlation coefficient between the gross motor function measurement and the gross motor function classification system, pediatric-evaluation-of-disability-inventory—mobility; confirmatory-factor-analysis was carried. Results: Internal-consistency (α: 0.997–1.00); reliability indices were excellent for total scale (intraclass-correlation-coefficient for intra-rater reliability 0.994–0.999, inter-rater reliability 0.997–0.999) and for each sub-dimension and total score. Standard-error-of-measurement was ranging 1.044–1.677, minimal-detectible-change was 2.435–5.520. Construct validity was supported by strong to excellent negative significant correlations (p < 0.05).
Optimisation of the Encapsulation of Grape Pomace Extract by Spray Drying Using Goat Whey Protein as a Coating Material
Authors: Perković Gabriela , Planinić Mirela , Šelo Gordana , Martinović Josipa , Nedić Radenko , Puš Matej , Bucić-Kojić Ana
Published: September 2024

The aim of this research was to determine the optimal conditions for the process of the microencapsulation of phenol-rich grape pomace extract (GPE) using spray drying and goat whey protein (GW) as a coating. The encapsulation was carried out with the aim of protecting the original bioactive components extracted from grape pomace to ensure their stability and protection from external agents, as well as antioxidant activity, during the conversion of the liquid extract into powder and during storage. Using the response surface methodology, an inlet air temperature of 173.5 °C, a GW ratio of 2.5 and a flow rate of 7 mL/min were determined as optimum process parameters. Under these conditions, a high yield (85.2%) and encapsulation efficiency (95.5%) were achieved with a satisfactorily low moisture content in the product (<5%). The amount of coating had the greatest influence on the MC properties. GW showed a more pronounced stabilising effect on the phenolic compounds in GPE during a longer storage period compared to anthocyanins. The results obtained indicate the potential of GW as a coating and are an example of the possible upcycling of GPE and GW, which can lead to a high-quality product that can be a functional ingredient.
Natural Selection on Hydroxyapatite Fiber Orientations for Resisting Damage of Enamel
Authors: Shen Junfu , Xin Haiyan , Li Xiaopan , Kong Yiyun , Zhu Siqi , Zhou Yuankai , Fan Yujie , Xia Jing
Published: September 2024

Teeth have excellent mechanical properties, with high wear resistance and excellent fracture resistance. This is due to their well-organized multilevel hierarchical structure. While a number of studies in the last decades have revealed the relationship between tooth structure and mechanical properties, there is still no general agreement on how different orientations of hydroxyapatite (HAp) fibers affect the mechanical properties of enamel. With a scanning electron microscope and nanoindenter, the orientations of HAp fibers and their properties were investigated. HAp fibers have two different orientations: parallel and perpendicular to the surface. Fibers oriented parallel to the surface exhibited higher hardness, elastic modulus and wear resistance. Under applied force, fibers oriented perpendicular to the surface suffered deeper shearing in the protein along the long axis, resulting in lower mechanical properties. Teeth resist damaging fractures by combining hard and soft structures. This study may lead to new insights into how nature selects for tooth structure and provide a theoretical basis for the bioinspired design.
A Randomization-Based, Model-Free Approach to Functional Neuroimaging: A Proof of Concept
Authors: Mazor Matan , Mukamel Roy
Published: September 2024

Functional neuroimaging analysis takes noisy multidimensional measurements as input and produces statistical inferences regarding the functional properties of brain regions as output. Such inferences are most commonly model-based, in that they assume a model of how neural activity translates to the measured signal (blood oxygenation level-dependent signal in the case of functional MRI). The use of models increases statistical sensitivity and makes it possible to ask fine-grained theoretical questions. However, this comes at the cost of making theoretical assumptions about the underlying data-generating process. An advantage of model-free approaches is that they can be used in cases where model assumptions are known not to hold. To this end, we introduce a randomization-based, model-free approach to functional neuroimaging. TWISTER randomization makes it possible to infer functional selectivity from correlations between experimental runs. We provide a proof of concept in the form of a visuomotor mapping experiment and discuss the possible strengths and limitations of this new approach in light of our empirical results.
Modelling and Evalaution of the Bidirectional Surge Current Robustness of Si(-IGBT and -Diode), SiC(-MOSFETs and -JFET) and GaN(-HEMTs) Devices
Authors: Nehmer Dominik , Ringelmann Tim , Bakran Mark-M.
Published: September 2024

This paper will evaluate the surge current robustness of different devices in relation to the active short circuit (ASC). For the purposes of this study, a Si IGBT and its diode, two SiC MOSFETs with different voltage ratings, a SiC JFET, and three GaN HEMTs will be compared. For the GaN devices, a eMode, a dMode, and a cascode device are employed. With the exception of the Si diode, all devices exhibited a current saturation effect. This saturation will result in significant losses and, ultimately, a thermal defect. For all devices, a safe operating area (SOA) criterion is established. For the SiC and GaN devices, the saturation voltage can be employed to define the safe operating area (SOA) criterion. In this context, two on-state resistance models will be defined for these devices. One is solely temperature-dependent, while the other also considers current saturation. Consequently, the saturation voltage and the on-resistance model represent a straightforward methodology for evaluating the ASC robustness of the devices. For all devices, a recommendation for a loss model and SOA criterion will be provided. Finally, the surge current robustness of all devices is compared. The Si, SiC and GaN devices exhibit comparable high surge current robustness in the application, with the exception of the GaN eMode, which is susceptible to strong current saturation.
Integration and Optimization of Multisource Electric Vehicles: A Critical Review of Hybrid Energy Systems, Topologies, and Control Algorithms
Authors: Fesakis Nikolaos , Falekas Georgios , Palaiologou Ilias , Lazaridou Georgia Eirini , Karlis Athanasios
Published: September 2024

Electric vehicles (EVs) are pivotal in addressing the escalating environmental crisis. While EV drivetrains excel compared to those of vehicles with internal combustion engines (ICEs), their energy storage systems are hampered by limited range, lifespan, and lengthy charging times. Hybrid energy storage systems (HESSs) present a viable current solution to these issues. This review thoroughly explores the state of the art in the emerging field of multisource EVs that utilize HESSs, incorporating any combination of batteries (BTs), supercapacitors (SCs), flywheels (FWs), fuel cells (FCs), and/or transmotors. In addition, the paper systematically categorizes and evaluates different hybrid configurations, detailing potential topologies and their respective advantages and limitations. Moreover, the paper examines diverse control algorithms used to manage these complex systems, focusing on their effectiveness and operational efficiency. By identifying current research gaps and technological challenges, this study aims to delineate future research directions that could enhance the deployment and optimization of multisource EVs, thereby addressing critical challenges such as energy density, system reliability, and cost-effectiveness.
Research on Phase Change Cold Storage Materials and Innovative Applications in Air Conditioning Systems
Authors: Li Zhengjing , Sha Yishun , Zhang Xuelai
Published: September 2024

Phase change cold storage materials are functional materials that rely on the latent heat of phase change to absorb and store cold energy. They have significant advantages in slight temperature differences, cold storage, and heat exchange. Based on the research status of phase change cold storage materials and their application in air conditioning systems in recent years, this paper provides an overview of the materials and their enhanced research progress. It summarizes the types of phase change cold storage air conditioning systems, optimization schemes, and system applications. This paper also identifies the current issues in phase change cold storage air conditioning and discusses the development trends in cold storage materials and air conditioning systems. It anticipates that future advancements will focus on composite phase change cold storage materials and low-energy consumption intelligent phase change cold storage air conditioning systems in steam compression using spherical capsules and concave–convex plate PCM.
Sensitivity Analysis and Distribution Factor Calculation under Power Network Branch Power Flow Exceedance
Authors: Sun Shuqin , Yuan Zhenghai , Liang Weiqiang , Qi Xin , Zhou Guanghao
Published: September 2024

As the scale of power systems continue to expand and their structure becomes increasingly complex, it is likely that branch power flow exceedance may occur during the operation of power systems, posing threats to the safe and stable operation of entire systems. This paper addresses the issue of branch flow exceedance in power networks. To enhance the operational efficiency and optimize the adjustment effects, this paper proposes a method for eliminating branch power flow exceedance by improving the particle swarm optimization (PSO) algorithm through the introduction of sensitivity and distribution factors. Firstly, it introduces the basic theory and calculation methods of sensitivity analysis, focusing on deriving the calculation principles of power flow sensitivity and voltage sensitivity, used to predict the responses of power flow at each branch in the power network to power or voltage changes. Subsequently, the paper provides a detailed derivation of the calculation principles for the line outage distribution factor (LODF), which effectively assesses the changes in branch power flow in the power network under specific conditions. Finally, a method for eliminating branch power flow exceedance based on a combination of sensitivity analysis and PSO algorithm is proposed. Through case analysis, it is demonstrated how to use the sensitivity and distribution factor to predict and control the power flow exceedance issues in power systems, verifying the efficiency and practicality of the proposed method for eliminating branch power flow exceedance. The study shows that this method can rapidly and accurately predict and address branch power flow exceedance in power system, thereby enhancing the operational safety of the power system.
Experimental Study on Temperatures of Water Walls in a 1000 MW Ultra-Supercritical Boiler under the Condition of Flexible Peak Regulation
Authors: Yan Liyun , Pu Jiang , Li Xueling , Lv Cai , Wu Xuehong , Li Liansheng , Lu Xiaofeng
Published: September 2024

To meet the Chinese government’s energy-saving and emission-reduction policies, flexible peak regulation is necessary for traditional coal-fired boilers. Flexible peaking leads to large changes in boiler load, which affects the safety of the boiler water wall. In this paper, a 1000 MW ultra-supercritical unit was tracked for three years, and effective data were selected to study the temperature characteristics of the water wall under flexible peak regulation. The results show that the lower the load, the greater the temperature fluctuation of the water wall. The temperature distribution of the spiral water wall is more uniform. The position of the temperature valley value of the rear spiral water wall was found, and the load of more even temperature distribution was also found. The temperature change of the front vertical water wall was the most complex of all the water walls. The 643.9 MW load case showed different behavior to the temperature distribution of the water wall. The side water walls were heated evenly under the different loads. The characteristics of the temperature distribution of the side vertical water wall were found through statistical analysis. The fitting equation for the change rule of the temperature is presented. The higher the load, the better the equations. Finally, this paper gives some advice on how to avoid temperature deviation in the water wall, and the detailed research highlights the safe running of water walls.
Mitigating Voltage Drop and Excessive Step-Voltage Regulator Tap Operation in Distribution Networks Due to Electric Vehicle Fast Charging
Authors: Hernández-Gómez Oscar Mauricio , Abreu Vieira João Paulo , Muñoz Tabora Jonathan , Sales e Silva Luiz Eduardo
Published: September 2024

Electric vehicles (EVs) are transforming the transportation sector, driven by the rapid expansion of charging infrastructure, including fast-charging stations (FCSs), significantly reducing charging time compared to standard charging stations. Despite the advantages of faster charging, the substantial power demand of EVs poses significant technical challenges for distribution networks. In particular, the existing literature has a research gap regarding how FCSs may impact or interact with step-voltage regulators’ (SVRs) tap operations. In this study, we characterize and evaluate the effects of fast recharging at varying penetration levels (PLs) on SVRs’ tap operations using probabilistic simulations and sensitivity analysis. To address these challenges, we propose a local and innovative application of the Volt/Var control on EV fast charging. The proposed application aims to inject reactive power into the network, depending on the FCS’s nominal active power, when the bus voltage connected to the FCS exceeds a minimum value. Our research on an actual feeder in northern Brazil reveals that reducing the active power supplied to the vehicle or oversizing the charging station power converters is unnecessary. Furthermore, our strategy reduces the probability of undervoltage violations and minimizes SVR tap changes, mitigating EVs’ impact on voltage quality.
The Application of the Particle Element Method in Tubular Propellant Charge Structure: Lumped Element Method and Multiple-Element Method
Authors: Tao Ruyi , Cheng Shenshen , Lu Xinggan , Xue Shao , Cui Xiaoting
Published: September 2024

Due to the orderly arrangement of tubular propellant, the permeability of combustion gases is improved, which is beneficial for enhancing the safety of the combustion system. However, current internal ballistic gas-solid flow calculation methods adopt a quasi-fluid assumption, which cannot accurately account for the characteristics of long tube shapes. Additionally, tubular propellants exhibit both overall movement and parameter distribution characteristics, necessitating the decoupling of gas and solid phases. These two deficiencies in previous studies have limited the effectiveness of gas-solid flow simulations for tubular propellant. This paper proposes a numerical calculation model suitable for tubular propellant charging based on the particle element method for internal ballistic two-phase flow. Firstly, considering the overall movement characteristics of tubular propellants, the concept of blank particle elements is introduced to represent pure gas phase regions. Then, based on computational requirements, the tubular propellants are divided to form the lumped element method and the multiple-element method. The moving boundary method is used to calculate the movement process of the propellant bed particle group and is compared with experimental results to verify the applicability of the two methods in tubular propellant beds. Analysis results show that the particle element method can effectively capture changes in the flow field inside the chamber and the position of tubular propellants. The lumped element method can quickly obtain the flow field distribution characteristics inside the chamber, while the multiple-element method can capture parameter distribution characteristics at different positions of the tubular propellants while ensuring overall movement.
SF6 Experimental Study on the Variation Characteristics of Closing Prebreakdown Duration during Contact Deterioration of Circuit Breakers
Authors: Ma Feiyue , Xu Chuxiong , Niu Bo , Wang Yu , Xiang Zhonghua , Wu Qiang , Bao Juming
Published: September 2024

The contact morphology change caused by high-current ablation will seriously affect the electric field distribution in the interrupter chamber, which in turn affects the closing prebreakdown arc duration, indicating that the prebreakdown arc duration can be used as one of the indicators to measure the contact ablation state. A circuit breaker simulated ablation test platform was established, and the voltage and current signals, electromagnetic field signals, and vibration signals in the process of circuit breaker closing were measured, and the closing prebreakdown duration was calculated. The results show that under the same size of the ablation current, with the ablation of the contacts, the closing prebreakdown duration shows an overall trend of increasing and then decreasing, and the larger the ablation current is, the larger the change in the closing prebreakdown duration is. At the same time, simulation verification was carried out, and the results show that the electric field distortion on the surface of the static arc contact inside the arc extinguishing chamber is the largest, and the ablation of the contact will further increase the degree of distortion of the electric field inside the arc extinguishing chamber. As the degree of ablation increases, the prebreakdown moment of the circuit breaker closing is advanced, and the prebreakdown duration increases.
Hybrid-Energy Storage Optimization Based on Successive Variational Mode Decomposition and Wind Power Frequency Modulation Power Fluctuation
Authors: Chen Changqing , Tang Weihua , Xia Yunqing , Chen Chang
Published: September 2024

In order to solve the problem of frequency modulation power deviation caused by the randomness and fluctuation of wind power outputs, a method of auxiliary wind power frequency modulation capacity allocation based on the data decomposition of a “flywheel + lithium battery” hybrid-energy storage system was proposed. Firstly, the frequency modulation power deviation caused by the uncertainty of wind power is decomposed by the successive variational mode decomposition (SVMD) method, and the mode function is segmented and reconstructed by high and low frequencies. Secondly, a mathematical model is established to maximize the economic benefit of energy storage considering the frequency modulation mileage, and quantum particle swarm optimization is used to solve the target model considering the charging and discharging power of energy storage and the charging state constraints to obtain the optimal hybrid-energy storage configuration. Finally, the simulation results show that, in the step disturbance, the Δfmax of the hybrid-energy storage mode is reduced by 37.9% and 15.3%, respectively, compared with single-energy storage. Under continuous disturbance conditions, compared with the single-energy storage mode, the Δfp_v is reduced by 52.73%, 43.72%, 60.71%, and 47.62%, respectively. The frequency fluctuation range is obviously reduced, and the frequency stability is greatly improved.
Railway Infrastructure Management: Selection of Overhead Contact Line Ampacity Considering Operational and Design Factors
Authors: Kuznetsov Valeriy , Hubskyi Petro , Rojek Artur , Ciekanowski Zbigniew
Published: September 2024

This paper presents a novel approach to determining the ampacity of DC and AC railway overhead contact lines, considering both operational and design factors. The ampacity of contact lines is influenced by several design parameters, including materials, cross section and the number of catenary and contact wires, etc. Additionally, the permissible long-term and short-term current values, which correspond to the permissible temperatures defined by EN 50119:2020, are affected by operational factors such as ambient temperature, solar radiation, heat exchange conditions, wind speed, wear of contact wires. Existing methods for determining the ampacity of overhead contact lines often fail to account adequately for these critical factors and lack the necessary procedures to calculate the 30 min ampacity required by EN 50119:2020. In response, the method proposed in this study adapts the procedures of IEEE 738-2012 to the specific needs of railway contact lines, taking into account the effect of contact wire wear on current distribution and overall ampacity. The proposed method offers a more realistic and practical approach to determining the ampacity of contact lines, thereby enhancing the reliability and efficiency of the traction power supply infrastructure. This method could be useful for railway infrastructure managers to ensure the safe and efficient operation under varying environmental and operational conditions.
Unveiling the Potential of Brazilian Eucalyptus for Transparent Wood Manufacturing via the Kraft Pulping Process as a Future Building Material
Authors: Barbosa Kelvin Techera , Cardoso Gabriel Valim , Pereira Acosta Andrey , Aramburu Arthur Behenck , Delucis Rafael de Avila , Gatto Darci A. , Labidi Jalel , Beltrame Rafael
Published: September 2024

The emergence of transparent wood as a viable alternative to traditional glass has sparked considerable interest in recent research endeavors. Despite advancements, challenges persist in the delignification methods and wood species utilized in prior studies. Therefore, this study delves into the potential of Brazilian eucalyptus wood for transparent wood production through the kraft pulping process. Delignification was carried out in a laboratory setting, replicating the kraft process with varying reaction times (15, 30, 45, and 60 min). The resulting delignified wood veneers were impregnated with a pre-polymerized PMMA solution. The study encompassed various analyses, including UV-Vis spectroscopy, colorimetry, SEM, optical microscopy, and mechanical property evaluations. The results revealed intriguing trends in terms of transparency, color changes, microstructural modifications, and mechanical properties as a function of delignification time. This work presents valuable insights into the transformative potential of eucalyptus wood, offering a deeper understanding of the interplay between wood modification and PMMA impregnation.
Expression, Purification, and Anti-UV Irradiation Effect of RsSOD on HCE-T Human Corneal Epithelial Cells
Authors: Fu Xucong , Jiang Zhuo , Bi Wenhui , Yang Zhecheng , Lu Weina , Chen Jianqing , Lyu Zhengbing , Nie Zuoming
Published: September 2024

Superoxide dismutase (SOD) is a class of enzymes that catalyze the disproportionation of superoxide anion radicals into hydrogen peroxide and oxygen. It can remove excessive free radicals in organisms and acts as a potent antioxidant, cleaning free radicals generated by radiation and protecting cells from oxidative damage. In this study, we obtained a MnSOD gene from the radiation-resistant bacterium Radiobacillus sp. (RsSOD) and constructed its recombinant expression vector through gene synthesis. The recombinant RsSOD protein was efficiently expressed using IPTG induction, and purified via repeated freezing and thawing, heating, and DEAE anion-exchange chromatography. The purified RsSOD exhibited an enzyme activity of 2072.5 U/mg. Furthermore, RsSOD was demonstrated to have robust resistance to high temperatures, acid, alkali, and artificial intestinal fluid. Further studies were performed to investigate the radiation resistance of RsSOD against ultraviolet (UV) irradiation in human corneal epithelial (HCE-T) cells. The results indicated that a low concentration of RsSOD (6.25 U/mL) could promote HCE-T cell proliferation and protect these cells from damage caused by both long-term and short-term UV exposure, effectively reducing apoptosis induced by short-term UV irradiation. These findings suggest that the RsSOD protein possesses significant anti-UV irradiation property and is expected to be a candidate for treating ocular radiation-related diseases.
Quantitative Trait Loci Mapping of Heading Date in Wheat under Phosphorus Stress Conditions
Authors: Yang Bin , Qiao Ling , Zheng Xingwei , Zheng Jun , Wu Bangbang , Li Xiaohua , Zhao Jiajia
Published: September 2024

Wheat (Triticum aestivum L.) is a crucial cereal crop, contributing around 20% of global caloric intake. However, challenges such as diminishing arable land, water shortages, and climate change threaten wheat production, making yield enhancement crucial for global food security. The heading date (HD) is a critical factor influencing wheat’s growth cycle, harvest timing, climate adaptability, and yield. Understanding the genetic determinants of HD is essential for developing high-yield and stable wheat varieties. This study used a doubled haploid (DH) population from a cross between Jinmai 47 and Jinmai 84. QTL analysis of HD was performed under three phosphorus (P) treatments (low, medium, and normal) across six environments, using Wheat15K high-density SNP technology. The study identified 39 QTLs for HD, distributed across ten chromosomes, accounting for 2.39% to 29.52% of the phenotypic variance. Notably, five stable and major QTLs (Qhd.saw-3A.7, Qhd.saw-3A.8, Qhd.saw-3A.9, Qhd.saw-4A.4, and Qhd.saw-4D.3) were consistently detected across varying P conditions. The additive effects of these major QTLs showed that favorable alleles significantly delayed HD. There was a clear trend of increasing HD delay as the number of favorable alleles increased. Among them, Qhd.saw-3A.8, Qhd.saw-3A.9, and Qhd.saw-4D.3 were identified as novel QTLs with no prior reports of HD QTLs/genes in their respective intervals. Candidate gene analysis highlighted seven highly expressed genes related to Ca2+ transport, hormone signaling, glycosylation, and zinc finger proteins, likely involved in HD regulation. This research elucidates the genetic basis of wheat HD under P stress, providing critical insights for breeding high-yield, stable wheat varieties suited to low-P environments.
The Roles of Proton-Sensing G-Protein-Coupled Receptors in Inflammation and Cancer
Authors: Justus Calvin R. , Marie Mona A. , Sanderlin Edward J. , Yang Li V.
Published: September 2024

The precise regulation of pH homeostasis is crucial for normal physiology. However, in tissue microenvironments, it can be impacted by pathological conditions such as inflammation and cancer. Due to the overproduction and accumulation of acids (protons), the extracellular pH is characteristically more acidic in inflamed tissues and tumors in comparison to normal tissues. A family of proton-sensing G-protein-coupled receptors (GPCRs) has been identified as molecular sensors for cells responding to acidic tissue microenvironments. Herein, we review the current research progress pertaining to these proton-sensing GPCRs, including GPR4, GPR65 (TDAG8), and GPR68 (OGR1), in inflammation and cancer. Growing evidence suggests that GPR4 and GPR68 are mainly pro-inflammatory, whereas GPR65 is primarily anti-inflammatory, in various inflammatory disorders. Both anti- and pro-tumorigenic effects have been reported for this family of receptors. Moreover, antagonists and agonists targeting proton-sensing GPCRs have been developed and evaluated in preclinical models. Further research is warranted to better understand the roles of these proton-sensing GPCRs in pathophysiology and is required in order to exploit them as potential therapeutic targets for disease treatment.
Mitochondrial Dysfunctions: Genetic and Cellular Implications Revealed by Various Model Organisms
Authors: Stańczyk Monika , Szubart Natalia , Maslanka Roman , Zadrag-Tecza Renata
Published: September 2024

Mitochondria play a crucial role in maintaining the energy status and redox homeostasis of eukaryotic cells. They are responsible for the metabolic efficiency of cells, providing both ATP and intermediate metabolic products. They also regulate cell survival and death under stress conditions by controlling the cell response or activating the apoptosis process. This functional diversity of mitochondria indicates their great importance for cellular metabolism. Hence, dysfunctions of these structures are increasingly recognized as an element of the etiology of many human diseases and, therefore, an extremely promising therapeutic target. Mitochondrial dysfunctions can be caused by mutations in both nuclear and mitochondrial DNA, as well as by stress factors or replication errors. Progress in knowledge about the biology of mitochondria, as well as the consequences for the efficiency of the entire organism resulting from the dysfunction of these structures, is achieved through the use of model organisms. They are an invaluable tool for analyzing complex cellular processes, leading to a better understanding of diseases caused by mitochondrial dysfunction. In this work, we review the most commonly used model organisms, discussing both their advantages and limitations in modeling fundamental mitochondrial processes or mitochondrial diseases.
Identification of Key Proteins Related to Cashmere Fiber Diameter by Integrated Proteomics and Bioinformatic Analyses in the Alpas and Alxa Goat Breeds
Authors: Zhang Chongyan , Qin Qing , Wang Yichuan , Wang Zhixin , Liu Zhihong
Published: September 2024

Background: Goats (Capra hircus) have always been a source of fiber for human use and hold an important place in international high-end textiles. Fiber diameter is the most concerning economic indicator for producers. Understanding the formation mechanism of fiber diameter and its related key proteins can help optimize and control the production of cashmere. Methods: Cashmere goats (n = 36) of the Alpas (n = 18) and Alxa (n = 18) breeds, with a similar age (2 years old) and live weight (25–26 kg), were selected from the Yiwei White Cashmere Goat Breeding Farm, Erdos, Inner Mongolia. Using phenotypic indicators, we evaluated the diameter of the cashmere fibers in Alxa and Alpas goats. We also used electron microscopy to examine the cashmere fiber’s structure and label-free liquid chromatography–tandem mass spectrometry to determine the protein content of the two cashmere fibers. The proteins affecting fiber diameter were identified and analyzed by Western blot, Co-Immunoprecipitation, and bioinformatics analysis. Results: The average diameter of the Alxa breed was smaller (p < 0.05) than that of the Alpas breed (Alxa’s cashmere vs. Alpas’ cashmere). Proteomics technology enabled the highly confident detection of 171 proteins. A total of 68 differentially expressed proteins were identified in the two types of cashmere; 131 proteins were specifically expressed in Alpas goats, and 40 proteins were specifically expressed in Alxa goats. A key protein group that could cause variations in fiber diameter was found using the protein–protein interaction network. To ascertain the reason for the variation in fiber diameter, a structural study of the major protein groups was carried out. Conclusions: KRT10, KRT14, KRT17, and KRT82 are the main proteins impacting the diameter difference, and they have a substantial effect on the average fiber diameter.
Virtual Reality in Cancer Care: Enhancing Knowledge and Reducing Anxiety about Chemotherapy among Patients and Caregivers
Authors: Thomas Melissa K. , Jarrahi Abolfazl (Abel) , Dennie Lauren , Scott Sam , Lau Ted , Johnson Annika
Published: September 2024

Virtual reality (VR) technology has evolved from entertainment to significant applications in healthcare and education. Despite its potential, there is limited research on the role of VR in cancer care. This study investigates VR’s ability to simulate the chemotherapy process, aiming to enhance patients’ knowledge and mitigate anxiety associated with chemotherapy. Utilizing a two-arm, mixed-methods pre/post-survey design, the study measured changes in patients’ anxiety and knowledge before and after exposure to a VR simulation. Participants (n = 267) engaged with VR simulations or interactive 360-degree videos depicting the chemotherapy process. Data analyses revealed a significant median increase in chemotherapy knowledge post-exposure to the VR content (z = 12.511, p < 0.001). Demographic factSors significantly influenced perceptions of VR realism and usefulness (p < 0.05). Additionally, VR exposure was correlated with reduced anxiety levels and improved treatment expectations (p < 0.05). Participants with higher post-understanding chemotherapy scores considered VR a useful tool for managing anxiety about chemotherapy and recommended VR for other medical procedures (p < 0.001). These findings underscore VR technology’s potential as a valuable tool in cancer treatment, suggesting it can enhance patient education and reduce anxiety, thereby improving patient outcomes during cancer therapy.
Age and Hair Cortisol Levels as Predictors of SARS-CoV-2 Infection
Authors: Fiedler Nancy , Ohman-Strickland Pamela , Shen Jialin Doris , Black Kathleen , Horton Daniel B. , Panettieri Reynold , Blaser Martin J. , Carson Jeffrey , Bendinskas Kestutis , Cheng Hana , Barrett Emily S.
Published: September 2024

Chronic psychosocial stress is known to adversely impact immune function. During the SARS-CoV-2 pandemic, occupational stress among workers in healthcare was at an unprecedented level due to risks of infection and work demands. We performed a nested case–control study to investigate the associations between chronic stress and the risks of contracting SARS-CoV-2. We collected 3 cm of hair from employees at an academic medical center who tested positive for SARS-CoV-2 (N = 49) and controls who tested negative (N = 49), matched for age, race, and sex. The diagnosis of SARS-CoV-2 was based on polymerase chain reaction or antibody tests. As a proxy for chronic stress, we segmented hair into 1 cm sections each representing one month and measured cortisol levels using a cortisol enzyme-linked immunosorbent assay. For cases, we used cortisol concentrations measured in hair segments from the month prior to a positive SARS-CoV-2 test, and for controls, we used time-matched hair segments. We fitted conditional logistic regression models adjusted for sex, age, race, body mass index, and healthcare worker status, and stratified models by older vs. younger age (cutoff = 41 years). African Americans had higher hair cortisol levels relative to participants of other races and ethnicities. In adjusted models, higher hair cortisol concentrations were associated with an increased odds of infection with SARS-CoV-2 (OR = 1.84; CI: 1.10–3.07) among older, but not younger, participants. The results suggest that psychosocial stress may be a risk factor for SARS-CoV-2 infection; stress management may be an important part of a comprehensive approach to protect against SARS-CoV-2 infection.
Toward Ensuring Data Quality in Multi-Site Cancer Imaging Repositories
Authors: Kosvyra Alexandra , Filos Dimitrios T. , Fotopoulos Dimitris Th. , Tsave Olga , Chouvarda Ioanna
Published: September 2024

Cancer remains a major global health challenge, affecting diverse populations across various demographics. Integrating Artificial Intelligence (AI) into clinical settings to enhance disease outcome prediction presents notable challenges. This study addresses the limitations of AI-driven cancer care due to low-quality datasets by proposing a comprehensive three-step methodology to ensure high data quality in large-scale cancer-imaging repositories. Our methodology encompasses (i) developing a Data Quality Conceptual Model with specific metrics for assessment, (ii) creating a detailed data-collection protocol and a rule set to ensure data homogeneity and proper integration of multi-source data, and (iii) implementing a Data Integration Quality Check Tool (DIQCT) to verify adherence to quality requirements and suggest corrective actions. These steps are designed to mitigate biases, enhance data integrity, and ensure that integrated data meets high-quality standards. We applied this methodology within the INCISIVE project, an EU-funded initiative aimed at a pan-European cancer-imaging repository. The use-case demonstrated the effectiveness of our approach in defining quality rules and assessing compliance, resulting in improved data integration and higher data quality. The proposed methodology can assist the deployment of big data centralized or distributed repositories with data from diverse data sources, thus facilitating the development of AI tools.
Efficacy and Safety in a Real-World Study of the New Oral Formulation of Semaglutide in Patients with Chronic Kidney Disease and Type 2 Diabetes Mellitus
Authors: Marques Vidas María , López-Sánchez Paula , Sánchez-Briales Paula , López Illazquez María Victoria , Portolés Jose
Published: September 2024

Background/Objectives: GLP-1 receptor agonists (GLP-1RAs) have emerged as fundamental components in the treatment of type 2 diabetic patients (T2DM) with chronic kidney disease (CKD). The oral formulation represents a novel therapeutic tool but may affect drug efficacy. This study sought to compare the effectiveness of subcutaneous versus oral semaglutide formulations in patients with CKD. Methods: A retrospective study in a real-world setting compared type 2 diabetes and chronic kidney disease patients, initiating oral semaglutide treatment to a historically matched control group treated with subcutaneous semaglutide. The matching considered factors such as estimated glomerular filtration rate (eGFR), age, and sex. Results: Nineteen patients were included in both groups, with a mean age of 68.0. Seventy-two percent were males with a CKD-EPI eGFR of 49.9 mL/min/1.73 m2 and a median urine albumin-to-creatinine ratio of 12.7 mg/g. Of the study participants, 94% and 79% of patients were on the maximum semaglutide sbc vs. oral dose, while 5.3% and 15.8% were on the sbc vs. oral low dose. Oral semaglutide significantly reduced HbA1C and BMI, identical to the control group (−0.9 and −1.4, p > 0.05). Renal function parameters and blood pressure remained stable throughout the follow-up in both groups. The main side effect was digestive intolerance (affecting three patients in the oral group and two patients in the subcutaneous group, p = 0.6), although the treatment abandonment percentage was similar. Conclusions: The oral formulation of semaglutide demonstrated equivalent effectiveness in glucose control and body weight management in patients with T2DM and CKD, even with a higher proportion of patients receiving low to medium doses. Gastrointestinal side effects were comparable between both oral and subcutaneous formulations.
Pain Assessment and Analgesic Requirements after Sleeve Gastrectomy: A Comparison Study of Robotic versus Laparoscopic Approaches
Authors: Barajas-Gamboa Juan S. , Ihsan Khan Mohammed Sakib , Mocanu Valentin , Dang Jerry T. , Romero-Velez Gustavo , Lee St-John Terrence , Diaz Del Gobbo Gabriel , Guerron A. Daniel , Pantoja Juan Pablo , Abril Carlos , Raza Javed , Rodriguez John , Kroh Matthew , Corcelles Ricard
Published: September 2024

Background: Sleeve gastrectomy is the most common bariatric procedure worldwide, yet postoperative pain management remains a concern. This study aimed to compare pain medication usage and pain scores between laparoscopic sleeve gastrectomy (LSG) and robotic sleeve gastrectomy (RSG) patients, addressing the potential benefits of RSG in postoperative pain control. Methods: A retrospective review of our institutional bariatric surgery registry included 484 patients (435 LSG, 49 RSG) who underwent surgery between September 2015 and November 2020. Pain management medications, including opioid use converted to morphine milligram equivalents (MMEs), and Numerical Rating Scale (NRS) scores were analyzed postoperatively using mixed-effects models. Results: RSG patients reported lower pain scores in the initial 24 h post-surgery and received significantly lower doses of morphine equivalents compared to LSG patients (median 7.5 mg vs. 12.5 mg, p < 0.001). RSG procedures had longer operative times (122.5 ± 27.1 vs. 89.9 ± 30.5 min, p < 0.001) but a shorter average length of stay (2.24 ± 0.60 vs. 2.65 ± 1.49 days) compared to LSG. Conclusions: These findings suggest that RSG may lead to reduced immediate postoperative pain and lower opioid requirements compared to the laparoscopic approach, despite longer operative times. Further randomized controlled trials are needed to confirm these observations and evaluate long-term outcomes.
Vascular Complications in Extracorporeal Membrane Oxygenation—A Narrative Review
Authors: Hart Joseph P. , Davies Mark G.
Published: September 2024

The establishment of a peripheral ECMO circuit can lead to significant arterial and venous complications in 10–30% of patients. Vascular complications, particularly acute limb ischemia, are associated with worsening overall outcomes. Limb ischemia occurs significantly more frequently in the early stages of VA ECMO than in VV ECMO. Mechanisms of limb ischemia include arterial obstruction, cannulation injury, loss of pulsatile flow, thromboembolism, venous stasis from compressive obstruction with large venous cannulas, and systemic vasoconstriction due to shock and pharmacologic vasoconstriction. The care team may use several mitigation strategies to prevent limb ischemia. Arterial and venous complications can be mitigated by careful access site selection, minimizing cannula size, placement of distal perfusion and/or outflow catheter(s), and continuous NIRS monitoring. Rapid intervention, when ischemia or compartment syndrome occurs, can reduce limb loss but may not affect the mortality and morbidity of the ECMO patient in the long term due to their underlying conditions and the etiology of the ECMO need.
Characterization of Super-Responder Profile in Chronic Plaque Psoriatic Patients under Guselkumab Treatment: A Long-Term Real-Life Experience
Authors: Marcelli Lorenzo , Belcastro Alfredo , Talamonti Marina , Paganini Claudia , Fico Angela , Savastano Lorenzo , Di Raimondo Cosimo , Vellucci Laura , Bianchi Luca , Galluzzo Marco
Published: September 2024

Background: The term “super responder” identifies a group of patients who exhibit a rapid and optimal response to biological treatment compared to the overall treated population. The primary objective of our study is to characterize this subgroup of patients to enable the early identification of those who will respond most effectively to the proposed treatment while also evaluating clinical efficacy. Methods: This retrospective study evaluated 232 patients treated with guselkumab in monotherapy for at least 20 weeks between November 2018 and November 2023. Patients were divided into two groups: those who achieved complete clear skin (PASI = 0) during the first 20 weeks of treatment were defined as super responders (SRe) and non-super responders (nSRe) were the remaining patients. PASI was assessed at weeks 0, 4, and subsequently every eight weeks. Predictors of the SRe status were evaluated by univariate and multivariate logistic regression analyses. Results: The univariate analyses showed that patients with psoriatic arthritis at the baseline, bio-naïve patients, or those who had not received an interleukin (IL) 17 inhibitor as their last therapy before guselkumab administration were more likely to be super responders to the proposed treatment. Multivariate logistic analysis models suggested that the combination of psoriatic arthritis at the baseline and the bio-naïve condition was the strongest predictive model for the SRe status. At week 204, the main difference between the two groups concerned the achievement of PASI100, maintained by 86.8 of SRe compared to 62.8% of nSRe. Conclusions: The efficacy and safety of guselkumab are confirmed in our real-life experience. Identifying the SRe status will undoubtedly play a role in clinical practice and the therapeutic decision-making algorithm.
A Tool Integrated into the Electronic Health Record to Guide Proper Decision-Making Regarding Peri-Endoscopic Anticoagulant Management: A Retrospective Cohort Study
Authors: Plender Anja , Graumans Suzanne E. , Gielisse Eric , Bresser-de Ruyter Carlinda , Sissing Simone , Ruiter-Jakobs Marjan C. , Wals Arian , Faber Laura M.
Published: September 2024

Background—Anticoagulants, such as vitamin-K antagonists (VKA) and direct oral anticoagulants (DOAC), are widely used among patients who undergo endoscopic procedures. To balance between bleeding and thromboembolic risks, careful decisions must be made about whether and for how long anticoagulants have to be stopped peri-endoscopically and if bridging is necessary. We created a tool in the electronic health records system (EHR) HIX (Microsoft) for invasive procedures to aid this decision-making. By selecting the anticoagulant indication or thrombo-embolic risk and the bleeding risk of the procedure, the tool automatically generates advice for periprocedural anticoagulant management. Objectives—This study assesses whether the tool is used properly peri-endoscopically. Secondly, it examines how many bleeding and thromboembolic events have occurred since the implementation of the tool. Methods—This retrospective study included all orders placed for endoscopies for patients using VKA or DOAC between 2018 and 2021. Results—In total, 986 endoscopies were included for analysis. In 89%, the tool was used correctly; the main error was selecting the wrong bleeding risk (7.5%). The cumulative incidence for moderate or severe bleeding events for DOAC and VKA was 2 (0.5%) and 0, respectively. The cumulative incidence of thromboembolic events for DOAC and VKA was 1 (0.2%) for each. Conclusions—This study evaluates the use of an EHR-integrated decision-making tool to aid peri-endoscopic anticoagulant management. By analysing the usage of the tool, we formulated several suggestions to improve the tool. Although this study is not a comparative one, we can conclude that the thromboembolic and major bleeding risks were low.