bluuebunny/crossref_metadata_2025 huggingface dataset:
Betaine Modulating MIF-Mediated Oxidative Stress, Inflammation and Fibrogenesis in Thioacetamide-Induced Nephrotoxicity
Authors: Jorgačević Bojan , Stanković Sanja , Filipović Jelena , Samardžić Janko , Vučević Danijela , Radosavljević Tatjana
Published: April 2022

Background:

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine
with chemokine properties released by various immune and non-immune cells. It contributes to the
pathogenesis of many inflammatory, autoimmune diseases and malignant tumors.

Objective:

Our study aimed to investigate the role of betaine in the modulation of MIF-mediated
oxidative stress, inflammation, and fibrogenesis during toxic kidney damage induced by thioacetamide
(TAA).

Methods:

The experiment is performed on wild-type and knockout MIF-/- C57BL/6 mice. They are
randomly divided into groups: Control; Bet-group, received betaine (2% wt/v dissolved in drinking
water); MIF-/- mice group; MIF-/- + Bet; TAA-group, treated with TAA (200 mg/kg b.w.), intraperitoneally,
3x/week/8 weeks); TAA+Bet; MIF-/-+TAA, and MIF-/- + TAA+Bet group. After eight
weeks of treatment, animals are sacrificed and kidney samples are taken to determine oxidative
stress parameters, proinflammatory cytokines, profibrogenic factors, and histopathology of renal
tissue.

Results:

In MIF-/-mice, TAA decreases malondialdehyde (MDA) concentration, IL-6, tumor necrosis
factor-alpha (TNF-α), transforming growth factor-beta 1 (TGF-β1) and platelet-derived growth
factor-BB (PDGF-BB) and increases superoxide dismutases (SOD) and catalase (CAT) activities, as
well as glutathione (GSH) content in kidneys, compared to TAA group. Betaine alleviates the
mechanism of MIF-mediated effects in TAA-induced nephrotoxicity, reducing MDA, IL-6, TNF-α,
TGF-β1, and PDGF-BB, and increasing SOD and CAT activity, as well as GSH levels.

Conclusion:

MIF mediates TAA-induced nephrotoxicity by increasing oxidative stress, inflammation,
and profibrogenic mediators. MIF-targeted therapy could potentially alleviate oxidative stress
and inflammation in the kidney, as well as pathohistological changes in renal tissue, but the exact
mechanism of its action is not completely clear. Betaine alleviates MIF nephrotoxic effects by increasing
the antioxidative capacity of kidney cells, and decreasing lipid peroxidation and cytokine
production in the renal tissue. It suggests that betaine can be used for the prevention of kidney damage.
Potential Protein Blood-based Biomarkers in Different Types of Dementia: A Therapeutic
Overview
Authors: Manzine Patricia R. , Vatanabe Izabela P. , Grigoli Marina M. , Pedroso Renata V. , Monteiro Maria Patricia A.O. , Oliveira Danielle S.M.S. , Nascimento Carla M.C. , Peron Rafaela , Orlandi Fabiana S. , Cominetti Márcia R.
Published: April 2022

Biomarkers capable of identifying and distinguishing types of dementia such as Alzheimer's disease (AD), Parkinson's disease dementia (PDD), Lewy body dementia (LBD), and frontotemporal dementia (FTD) have been become increasingly relentless. Studies of possible biomarker proteins in the blood that can help formulate new diagnostic proposals and therapeutic visions of different types of dementia are needed. However, due to several limitations of these biomarkers, especially in discerning dementia, their clinical applications are still undetermined. Thus, the updating of biomarker blood proteins that can help in the diagnosis and discrimination of these main dementia conditions is essential to enable new pharmacological and clinical management strategies, with specificities for each type of dementia. To review the literature concerning protein blood-based AD and non-AD biomarkers as new pharmacological targets and/or therapeutic strategies. Recent findings for protein-based AD, PDD, LBD, and FTD biomarkers are focused on in this review. Protein biomarkers were classified according to the pathophysiology of the dementia types. The diagnosis and distinction of dementia through protein biomarkers is still a challenge. The lack of exclusive biomarkers for each type of dementia highlights the need for further studies in this field. Only after this, blood biomarkers may have a valid use in clinical practice as they are promising to help in diagnosis and in the differentiation of diseases.
Sparteine Family: Privileged Chiral Ligands in Transition Metal Catalysis
Authors: Zhang Mingyu , Yan Yuting , Sun Hexin , Fu Xuegang , Huang Jianhui
Published: April 2022

Chiral ligands are the footstones for asymmetric synthesis, constructing the enantioenriched
molecules that are widely used in material and medicinal sciences. Naturally occurring
compounds, such as proline analogues and cinchona alkaloids, are widely used as privileged
ligands in asymmetric synthesis. Sparteine, a natural alkaloid firstly reported in 1968,
was also employed in asymmetric synthesis, albeit with less satisfactory results. In this perspective,
transition metal-involved asymmetric transformations using sparteine or family
members as ligands are overviewed and discussed. The design and perspective of ligands with
similar skeleton are also proposed.
Pegfilgrastim-Apgf (Nyvepria): Biosimilar USFDA Approval for the
Treatment of Chemotherapy-induced Febrile Neutropenia and Current
Updates on Clinical Trials
Authors: Ali Faraat , Sharma Kamna , Ali Asad
Published: April 2022

Pegfilgrastim-apgf (nyvepria) was currently approved by FDA for the treatment of febrile neutropenia associated with non-myeloid malignancies receiving myelosuppressive anticancer drugs. It was developed by Pfizer, USA. It is a PEGylated leukocyte growth-stimulating factor indicated to reduce the incidence of febrile neutropenia in patients receiving anticancer drugs. Nyvepria is biosim-ilar to pegfilgrastim, approved by FDA on June 10, 2020. It is the fourth FDA-approved drug for the treatment of infection exhibiting febrile neutropenia. This review abridges the indicators in the devel-opment of nyvepria foremost to approval for the treatment of febrile neutropenia (FN), a biosimilar regulatory framework, and current updates on the clinical trials (CTs).
Sperm Mitochondria, the Driving Force Behind Human Spermatozoa Activities: Its Functions and Dysfunctions - A Narrative Review
Authors: Kumar Naina
Published: April 2022

Male infertility is a major issue, and numerous factors contribute to it. One of
the important organelles involved in the functioning of human spermatozoa is
mitochondria. There are 50-75 mitochondria helically arranged in mid-piece bearing one
mitochondrial DNA each. Sperm mitochondria play a crucial role in sperm functions,
including the energy production required for sperm motility and the production of reactive
oxygen species, which in the physiological range helps in sperm maturation,
capacitation, and acrosome reaction. It also plays a role in calcium signaling cascades,
intrinsic apoptosis, and sperm hyperactivation. Any structural or functional dysfunction of
sperm mitochondria results in increased production of reactive oxygen species and, a
state of oxidative stress, decreased energy production, all leading to sperm DNA
damage, impaired sperm motility and semen parameters, and reduced male fertility.
Furthermore, human sperm mitochondrial DNA mutations can result in impaired sperm
motility and parameters leading to male infertility. Numerous types of point mutations,
deletions, and missense mutations have been identified in mtDNA that are linked with
male infertility.

Methods:

Recent literature was searched from English language peer-reviewed journals
from databases including PubMed, Scopus, EMBASE, Scholar, and Web of Science till
September 2021. Search terms used were “Sperm mitochondria and male fertility”,
“Bioenergetics of sperm”, “Sperm mitochondria and reactive oxygen species”, “Sperm
mitochondrial mutations and infertility”.

Conclusion:

Sperm mitochondria is an important organelle involved in various functions
of human spermatozoa and sperm mitochondrial DNA has emerged as one of the potent
biomarkers of sperm quality and male fertility.
Current Novel Drug Deliveries for Oral Cancer: A Chronotherapeutic
Approach
Authors: Sutar Kishori P. , Shirkoli Nisha S. , Sutar Prasanna S. , Kurangi Bhaskar K. , Dandagi Panchaxari M. , Masareddy Rajashree
Published: April 2022

Oral squamous cell carcinoma is a malignant disease that is causing considerable mortality
worldwide. Conventional treatment approaches, like surgery, cause destructive alterations in facial appearance
and oral function impairments associated with psychological and social functioning. Chemotherapy
exhibits low bioaccessibility of the anticancer drugs, multiple drug resistance, higher dose necessities,
which elevate toxicities to the normal cells, low therapeutic index, and non-specific targeting.
Radiation therapies significantly affect the well-being of the patient and impair the quality of life.
Therefore, chemotherapeutics are developed that can either actively or passively target the carcinomas,
reduce the adverse side effect, and improve therapeutic efficacy. Innovations in novel drug delivery
systems deliver the drugs to the desired site of action with better treatment approaches with reduced
toxicities to the normal cells and improve the health and survival rate of the patient. Cancer chronotherapy
enhances the treatment proficiency by administration of the drugs at the best time, considering biological
timings to improve the treatment profiles. Chronotherapy provides benefits to the current anticancer
therapies, with minimum adverse effects to the healthy cells. This review discusses the risk factors
for oral carcinomas, targeted therapy by nanocarriers, nanotechnology approaches, the role of circadian
rhythm in the management of oral cancer, and advances in controlled drug delivery.
Effect of Medium on the Activity of Eugenol: A Mini-Review
Authors: Rege Sameera A. , Varshneya Megha A. , Momin Shamim A.
Published: April 2022

The role of essential fatty acids is vital in the life of a human being. The lack of essential
fatty acids causes various diseases, such as cardiovascular, inflammatory bowel, and obesity.
Although synthetic antioxidants have already been used, nowadays, the demand for natural antioxidants
has increased mainly because of the adverse effects of synthetic antioxidants. Spices are
widely used in this context as a source of natural antioxidants. It has been observed that they also
act as pro-oxidants. This review aims to evaluate the fundamental reason for the different activities
of the spices by studying the example of eugenol, a principal phenolic constituent of clove oil. The
chemistry concerning the mechanism to display antioxidant and pro-oxidant activity is currently
being reviewed. The isomerization of eugenol to isoeugenol plays a key role in exhibiting dissimilar
behaviour of the eugenol. Under the polar medium, isomerization of eugenol does not take
place, and it acts as an antioxidant. However, in the non-polar medium, eugenol isomerizes to
isoeugenol and displays a pro-oxidant effect. In the basic medium, eugenol isomerizing to isoeugenol
undergoes degradation. Hence, to utilize the beneficial activity of eugenol, it should be present
in a polar or acidic medium. It can be concluded from this review article that the structural
form of any molecule plays a major role in utilizing its activity and is also dependent on the medium.
Hence, the medium also performs a vital role in influencing the activity of any molecule.
Selective Adsorption of Iron(III) Ions Based on Nickel(II) Oxide-copper(II)
Oxide Nanoparticles
Authors: Danish Ekram Y. , Marwani Hadi M. , Almoslehi Kholoud F. , Khan Sher Bahadar , Bakhsh Esraa M. , Asiri Abdullah M. , Abozenadah Hadeel A.
Published: April 2022

Background:

Water contamination and its remediation are currently considered a major concern
worldwide. Design of effective methods for water purification is highly demanded for the adsorption
and removal of such pollutants.

Objective:

This study depicts the effectiveness of nickel oxide-copper oxide nanoparticles (NiO-CuO),
which can extract and remediate ferric ions, Fe(III), from aqueous solutions.

Methods:

The NiO-CuO nanoparticles were simply prepared by the co-precipitation method and then
used as adsorbent with respectable advantages of high uptake capacity and surface area.

Results:

Adsorption of Fe(III) onto NiO-CuO nanoparticles showed an uptake capacity of 85.86 mgg-1 at
pH 5.0. The obtained data from the carried-out experiment of Fe(III) adsorption onto NiO-CuO nanoparticles
were well suited to the Langmuir isotherm and pseudo-second-order kinetic models. Moreover,
different coexisting ions did not influence the adsorption of Fe(III) onto NiO-CuO nanoparticles. The
recommended methodology was implemented on the adsorption and removal of several environmental
water samples with high efficiency.

Conclusion:

The designed method displayed that NiO-CuO nanoparticles can be used as a promising
material for the adsorptive removal of heavy metals from water.
Nomogram for Prediction of Hepatocellular Carcinoma Prognosis
Authors: Yang Shuai , Zhang Jiangang , Wang Jingchun , Xu Yanquan , Zhao Huakan , Lei Juan , Zhou Yu , Chen Yu , Wu Lei , Zhou Mingyue , Li Dingshan , Wang Enwen , Li Yongsheng
Published: April 2022

Background:

Hepatocellular Carcinoma (HCC) is associated with high mortality rates and
requires the identification of new therapeutic targets. We sought to develop a nomogram for reliably
predicting HCC prognosis.

Methods:

Gene expression was analyzed in R software, while the hub genes were defined as overlapping
candidates across five datasets. A prognostic nomogram was constructed using multivariate Cox
analysis and evaluated by receiver operating characteristic curve and concordance index analysis. The
fractions of tumor microenvironment cells were determined by using xCell. Hypoxia scores were calculated
by single-sample gene set enrichment analysis. Statistically, significance and correlation analyses
were processed in R.

Results:

Tow hub genes were identified, and a prognostic nomogram was established and evaluated in
the internal validation dataset (Area Under the Curve [AUC] 0.72, 95% Confidence Interval [CI] 0.63-
0.81) and external cohorts (AUC 0.70, 95% CI 0.55-0.85). The risk scores of the prognostic model were
positively and negatively correlated with fractions of the T helper 2 (Th2) cells (R = 0.39, p <0.001) and
the hematopoietic stem cells (R = -0.27, p <0.001) and Endothelial Cells (ECs; R = -0.24, p <0.001), respectively.
Angiogenesis was more active in the high-risk group, accompanied by increased proliferation
of ECs. Furthermore, the significance of Hypoxia-Inducible Factor 1-Alpha (HIF1A) gene-related
hypoxia in predicting HCC prognosis was demonstrated.

Conclusions:

A robust prognostic nomogram for predicting the prognosis of patients with HCC was developed.
The results suggested that Th2 cells, VEGF-related angiogenesis and HIF1A-related hypoxia
may be promising therapeutic targets for prolonging the overall survival of HCC patients.
Recent Advances in Epidermal Growth Factor Receptor Inhibitors (EGFRIs) and
their Role in the Treatment of Cancer: A Review
Authors: Unnisa Aziz , Chettupalli Ananda Kumar , Hussain Talib , Kamal Mohammad Amjad
Published: April 2022

Tyrosine kinases are known to play a role in tumour growth and proliferation, and they have become common drug targets. Tyrosine kinase inhibitors (TKIs) prohibit associated kinases from phosphorylating tyrosine residues in their substrates, preventing downstream signaling pathways from being activated. Multiple robust and well-tolerated TKIs targeting single or multiple targets, including EGFR, ALK, ROS1, HER2, NTRK, VEGFR, RET, MET, MEK, FGFR, PDGFR, and KIT, have been developed over the last two decades, contributing to our understanding of precision cancer medicine based on a patient's genetic alteration profile. The epidermal growth factor receptor (EGFR) family consists of four transmembrane tyrosine kinases (EGFR1/ErbB1, Her2/ErbB2, Her3/ErbB3, and Her4/ErbB4) and thirteen polypeptide ligands produced by them. Multiple solid tumours, including breast, pancreatic, head and neck, kidney, vaginal, renal, colon, and non-small cell lung cancer, overexpress EGFRs. Overexpression of these genes stimulates downstream signaling channels, causing cell proliferation, differentiation, cell cycle progression, angiogenesis, cell motility, and apoptosis inhibition. EGFRs' high expression and/or adaptive activation coincides with the pathogenesis and development of many tumours, making them appealing candidates for both diagnosis and therapy. Several strategies for targeting these receptors and/or the EGFR-mediated effects in cancer cells have been established. The majority of methods include the development of anti-EGFR antibodies and/or small-molecule EGFR inhibitors. This review presents the recent advances in EGFR TKIs and their role in the treatment of cancer.
Molecular Study of Integrase Gene I and Integrase Gene II in Clinical
Isolates of Pseudomonas aeruginosa
Authors: Zaki Maysaa El Sayed , Mahmoud Noha Mostafa , Rizk Mohamed Anies
Published: April 2022

Background:

The presence of the class I integron gene is associated with the emergence of
multiple drug resistance (MDR) phenotype in Pseudomonas aeruginosa (P. aeruginosa) isolates.

Aim:

The objectives of this research were to study the prevalence of integrase genes I (Intel I) and
integrase genes II (Intel II) in clinical isolates of P. aeruginosa and its association with antibiotic resistance
in these isolates.

Methods:

The study was a retrograde cross-sectional study that was carried out on 150 clinical isolates
of P. aeruginosa isolated from patients with healthcare-associated infections. The isolates were subjected
to biochemical identification and antibiotic sensitivity study by discs diffusion test. Intel I &
Intel II genes were detected by polymerase chain reaction (PCR).

Results:

Intel I gene was present in 48% of the isolates, and Intel II was present in 1.3% of the isolates.
Intel I gene was detected at a statistically significant high rate in MDR- P. aeruginosa (76.9%,
P=0.001) compared to non-MDR- P. aeruginosa (3.4%), while intel II had a statistically insignificant
increase in MDR- P. aeruginosa (1.1%, P=1.00) compared to non-MDR-P. aeruginosa (1.7%). Both
Intl I/Intl II genes were detected in 2.2% of MDR-P. aeruginosa isolates and were absent in non-
MDR-P. aeruginosa isolates with statistically insignificant difference (P=1.00). P. aeruginosa isolates
with Intel I gene had an increase in antibiotic resistance pattern to the used antibiotics discs. However,
this increase had statistically significant rates only for gentamicin (63.9%, P≤0.001), meropenem
(47.2%, P=0.009), trimethoprim/sulfamethoxazole (37.5%, P=0.013) and imipenem (44.4%, P=0.025).

Conclusion:

The present study highlights the high prevalence of the Intel I gene in clinical isolates of
P. aeruginosa, while the Intel II gene was less prevalent in these isolates. There was a significant association
between the prevalence of the Intel I gene and the MDR phenotype of P. aeruginosa and resistance
to gentamicin, meropenem, trimethoprim/sulfamethoxazole, and imipenem. These findings
need future evaluation in a higher number of clinical isolates of P. aeruginosa.
How Do Abnormalities in the Cerebrospinal Fluid Impact Neuropsychology
with Progressing Age?
Authors: Koh Rhun Yian , Joshi Gargi , Ling Anna Pick Kiong , Chye Soi Moi
Published: April 2022

The behavior of an individual changes from neonate to elderly due to the development of
the central nervous system (CNS). One of the important components of the CNS is the cerebrospinal
fluid (CSF), which bathes the brain and spinal cord. CSF has changing properties throughout life, including
composition and volume imbalance. However, a specific age group that shows prevailing abnormality-
corresponding behavior remains unclear. The objective of this article is to explore how such
changes reflect on one’s psychological as well as physical processing. Production of CSF could be affected
by many factors, including its flow, absorption, volume, and composition. Prenatally, congenital
malformations and infections hold the greatest risk of impacting the child’s physical and mental
growth. In adolescents, transmission of external substances like alcohol or drugs in the cerebrospinal
fluid is known to impact severe mood changes that potentially result in suicide and depression. In the
adult working population, the influence of stress levels on CSF composition causes anxiety and sleep
disorders. Finally, the reduced production of CSF was found to be associated with memory deficits
and Alzheimer’s disease in the aging group. From the collected evidence, it can be observed that CSF
played an important role in behavioral changes and may be associated with neurodegenerations. By
linking the CSF abnormalities to the clinical symptoms at different stages of life, it may provide additional
information in the diagnosis of diseases that are associated with neuropsychological changes.
Oral Mucosal In Vitro Cell Culture Model to Study the Effect of Fructilactobacillus
Phage on the Interplay between Food Components and Oral
Microbiota
Authors: Ghadimi Darab , Ebsen Michael , Röcken Christoph , Fölster-Holst Regina , Groessner-Schreiber Birte , Dörfer Christof , Bockelmann Wilhelm
Published: April 2022

Background and Aims: Dietary habits, food, and nutrition-associated oral dysbiosis lead to the
formation of microbial biofilm, which affects the overall health of an individual by promoting systemic
diseases like cardiovascular disease, immunological disorders, and diabetes. Today's diets contain a variety
of fermentable carbohydrates, including highly processed starch and novel synthetic carbohydrates such as
oligofructose, sucralose, and glucose polymers. These constitute risk factors in the initiation and progression
of oral dysbiosis. Oral, lung and gut microbiomes are interlinked with each other via direct and indirect
ways. It is unknown whether or not lactobacilli and Lactobacillus phages are able to rescue dysbiotic effects
by decreasing the uptake into the cells of excess simple sugars and their derivatives present within the
digestive tract.

Materials and Methods: Using transwell cell culture plate inserts, six groups of in vitro co-cultured TR146
and HepG2 cells, grown in DMEM medium either with or without sucrose (10 % v/v), were treated with
1) PBS, 2) Fructilactobacillus sanfranciscensis (F.s) H2A, 3) F.s H2A and sucrose, 4) F.s H2A plus sucrose
plus phage EV3 lysate, 5) F.s H2A plus sucrose plus phage EV3 supernatant, and 6) F.s H2A plus
sucrose plus phage EV3 particles. The pH of the culture medium (indicating lactic acid production) and
key oral biomarkers, including cytokines (IL-1β and IL-6), inflammatory chemokines (e.g., CXCL8 and
CCL2), and homeostatic chemokines (e.g., CXCL4 and CCL18) were measured.

Results: Excess sucrose significantly enhanced inflammatory signal molecules (e.g., IL-1β, IL-6, and
CCL2) secretion, concomitant with the enhancement of intracellular triglycerides in co-cultured HepG2
cells. Co-culture with F.s H2A decreased the sucrose-induced release of inflammatory signal molecules
from co-cultured cells, these effects being abolished by F.s phage EV3.

Conclusion: This study shows that Lactobacillus phages apparently influence the interplay between food
components, oral microbiota, and the oral cellular milieu, at least in part by affecting the microbial uptake
of excess free simple sugars from the oral milieu. To confirm the biological consequences of these effects
on human oral microbiota and health, further studies are warranted, incorporating ex vivo studies of human
dental plaque biofilms and host biomarkers, such as cytohistological, molecular, or biochemical measurements.
Synthesis and Biological Evaluation, Structural Elucidation, Thermogravimetric
Analysis, X-Ray Diffraction Studies of a Schiff Base Derived from
3-formylchromone and 3-aminoquinoline and their Cu(II) and Co(II) Complexes
Authors: Ghumbre Sushil K. , Patil Amol V. , Renge Atul S. , Dake Satish A. , Khade Bhimrao C.
Published: April 2022

Introduction:

The study of novel Schiff bases and their metal complexes has
achieved enormous attention of inorganic as well as medicinal chemists.

Objective:

The objective of this study is to study the structural elucidation and antimicrobial
screening of 3-formylchromone and 3-aminoquinoline-based Schiff base and their metal complexes.

Methods:

Cu(II) and Co(II) complexes of 3-((quinolino-3-ylimino) methyl)-4H-chromen-4-
one ligand were synthesized and characterized by elemental analysis, molar conductivity
measurement, infrared, UV-Visible, 1H NMR spectral studies, thermogravimetric analysis,
and powder X-ray diffraction studies.

Results:

Antibacterial activity of synthesized compounds were screened against Klebsiella
pneumoniae, Staphylococcus aureus, and Proteus vulgaris, and antifungal activity was
screened against fungi Candida albicans and Aspergillus niger. Schiff base ligand and their
Cu(II) and Co(II) complexes revealed significant antibacterial and antifungal activity against
tested strains. Octahedral geometry of metal complexes was proven by analytical, physical,
and spectral data.

Conclusion:

In this present work, novel Schiff base 3-((quinolino-3-ylimino) methyl)-4Hchromen-
4-one and its Cu(II) and Co(II) complexes revealed promising antibacterial and antifungal
activities.
Biogenic ZnO Nanoflowers: As an Alternative Antibacterial Nanomedicine
Authors: Tripathi Ravi Mani , Pudake Ramesh Namdeo , Shrivastav Braj Raj , Shrivastav Archana
Published: April 2022

Background:

Zinc oxide (ZnO) nanoparticles have been widely investigated for the development of next-generation nano-antibiotics against a broad range of microorganisms including multi-drug resistance. The morphology of nanomaterials plays an important role in antibacterial activity.

Objective:

The research goal is focused on the development of a low-cost antibacterial agent.

Method:

The biosynthesis method was used to make ZnO nanoflowers. The antibacterial activity of these biogenic ZnO nanoflowers was analyzed by three methods: growth curve, well diffusion, and colony-forming unit count (CFU) assays.

Results:

The assay methods used in this study confirmed the antibacterial activity of ZnO nanoflowers. The growth curve shows that 0.5 mg/mL concentration of ZnO nanoflowers acted as an effective bactericide as no significant optical absorption and virtually bacterial growth were ob-served. The inhibition zone was found at 25 mm at 70 μg of ZnO nanoflowers.

Conclusion:

The unique, simplistic, environmental-friendly, and cost-effective biosynthesis method was established for the ZnO nanoflowers using biomass of Bacillus licheniformis. The resulted ZnO nanoflowers show excellent antibacterial activity which could be used as an alternative to antibiot-ics in therapeutic processes.
COVID-19 Stress on Mental and Hair Health: A Marker for Diseases in
the Post-Pandemic Era
Authors: Accorsi Daniela Xavier , dos Santos Ingrid Aparecida Mendes , Accorsi Juliana Xavier , Abelan Ursulandréa Sanches , Júnior Celso Martin , Bohac Silvia , Bumiller-Bini Valéria , Boldt Angelica Beate Winter , Chin Chung Man
Published: April 2022

The seriousness of the COVID-19 pandemic with accumulating stress factors, including lack of
pharmacotherapy, quarantine, social distancing, delay of vaccination, and economic uncertainties, may
foster fear and psychiatric disorders that can precipitate or aggravate hair/scalp disease. Hair loss can lead
to decreased self-esteem, potentiating the negative effects on social life and generating a vicious cycle of
stress during the pandemic. The relationship between environment and behavior can also trigger epigenetic
changes in diseases, which may influence the health of the next generations. In this review, we describe
the interaction between the physiological mechanisms of stress on hair follicles and hair disorders and
openly discuss during pandemic/post-pandemic (not genetically determined but epigenetically triggered)
hair loss as a point of concern as a health marker for further development of chronic diseases, such as
diabetes, obesity, psychiatric disorders, and others.
The subject of proof in civil cases on the protection of privacy
Authors:   N.Sh. Gadzhialieva Daghestan State University, Gadzhialieva N.Sh.
Published: April 2022

The problematic of the subject of proof in cases of protection of privacy has been little developed in the doctrine of civil procedural law and therefore needs research. The article deals with the formation of the subject of proof in cases of protection of privacy in civil proceedings. Based on the analysis of the current Russian legislation, the legal positions of the higher courts, the author outlined the range of facts that constitute the subject of proof in the studied category of cases. The author notes that the European Court of Human Rights plays an important role in the formation of the correct factual composition in cases of protection of privacy, which forms the boundaries of a person's private life in accordance with the provisions of the European Convention for the Protection of Human Rights and Fundamental Freedoms. In conclusion, the author comes to the conclusion that the norm of civil law that determines the subject of proof in cases of protection of privacy is a discretionary norm, which complicates the determination of the range of facts that have legal significance in the case. In this regard, the author proposes to adopt a Resolution of the Plenum of the Supreme Court of the Russian Federation, in which clarifications will be given regarding the mechanism for forming the subject of evidence in cases of protection of privacy.
Criminal legal framework for countering illegal and unjustified hospitalization in a psychiatric hospital
Authors:   V.N. Voronin Moscow State Law University named after O.E. Kutafin (MSLA), Voronin V.N.
Published: April 2022

The subject of the research is public relations in the field of ensuring the physical freedom of the individual from illegal and unjustified hospitalization in a psychiatric hospital by criminal legal means. The author reveals certain problems of the emergence of a criminal law prohibition provided for by Art. 128 CC, its evolution and application. The research methodology is based on the general historical method and particular methods of legal analysis and legislative technique. As a conclusion, the author proposes a solution to theoretical problems regarding the establishment of signs of corpus delicti under Art. 128 of the Criminal Code, and also formulates proposals for improving the current criminal legislation and the practice of its application.
Webáruházak fogyasztói megítélésének vizsgálata netnográfiával a COVID-19 időszakában
Authors: Balogh-Kardos Valentina , Gál Tímea
Published: April 2022

Az online vásárlás egyre inkább a hétköznapjaink részévé válik, melyet az is bizonyít, hogy a koronavírus-járvány okozta gazdasági válság közepette az online kiskereskedelem 30%-ot meghaladó növekedést ért el Magyarországon. Nemzetközi kutatások igazolták, hogy a fogyasztók közel 90%-a vásárlásaikat megelőzően szinte ugyanannyira veszik figyelembe az online véleményeket, mint a családtagok vagy barátok ajánlásait, döntéseikre pedig a digitális csatornák, ezen belül is a Facebook van a legnagyobb hatással. Kutatásunkban egy konkrét akcióhoz, a Black Friday-hez kapcsolódóan arra a kérdésre kerestük a választ, hogy a Magyarországon működő legnagyobb forgalommal bíró, online webáruházzal is rendelkező üzletek, mennyire aktívan használják a Facebook közösségi oldalt a fogyasztók elérésére, illetve befolyásolására. Vizsgálatunk módszeréül a netnográfiát választottuk, mellyel az áruházak Facebook oldalain a Black Friday hetében megjelenő posztokat, az arra adott fogyasztói reakciókat, illetve hozzászólásokat elemeztük. Eredményeink azt mutatják, hogy az általunk kiválasztott öt webáruház közül a három legnagyobb forgalommal bíró alkalmazott kidolgozott Facebook kampányt az akció keretein belül. A hozzászólásokat elemezve a fogyasztókat 5 kategóriába soroltuk be az elégedettség és a bizalom alapján: az elégedettek, az elégedetlenek, az érdeklődők, trollok, illetve a némák csoportjába.
ipar 4.0 és a digitalizáció szerepe a mezőgazdaságban, különös tekintettel a romániai mezőgazdaságban
Authors: Nagy Szeréna
Published: April 2022

A robotizáció, az automatizáció, az adatok felhasználása, a mesterséges intelligencia nagymértékben teret hódítanak a világban és a mezőgazdasági szektorban is, amelyek hozzájárulnak egy hatékonyabb mezőgazdasági szektor kialakításához. A mezőgazdaságban a fenntartható fejlődés nélkülözhetetlen eszközei az ipar 4.0 által nyújtott lehetőségek, technológiák. Az agrárszektor számára a digitalizáció jelenti a jövőt, ugyanis növeli a terméshozamot, miközben a környezettermelés mérséklődik, nem növekedik. Jelen kutatás célja, hogy bemutassa az Ipar 4.0 fogalmát, szerepét a mezőgazdaságban, illetve megvizsgálja a romániai mezőgazdasági szektor viszonyulását, körülményeit az ipar 4.0 technológiáinak és a digitalizáció adoptálásához.
Improving Web-Based Treatment Intake for Multiple Mental and Substance Use Disorders by Text Mining and Machine Learning: Algorithm Development and Validation
Authors: Wiegersma Sytske , Hidajat Maurice , Schrieken Bart , Veldkamp Bernard , Olff Miranda
Published: April 2022

Background

Text mining and machine learning are increasingly used in mental health care practice and research, potentially saving time and effort in the diagnosis and monitoring of patients. Previous studies showed that mental disorders can be detected based on text, but they focused on screening for a single predefined disorder instead of multiple disorders simultaneously.

Objective

The aim of this study is to develop a Dutch multi-class text-classification model to screen for a range of mental disorders to refer new patients to the most suitable treatment.

Methods

On the basis of textual responses of patients (N=5863) to a questionnaire currently used for intake and referral, a 7-class classifier was developed to distinguish among anxiety, panic, posttraumatic stress, mood, eating, substance use, and somatic symptom disorders. A linear support vector machine was fitted using nested cross-validation grid search.

Results

The highest classification rate was found for eating disorders (82%). The scores for panic (55%), posttraumatic stress (52%), mood (50%), somatic symptom (50%), anxiety (35%), and substance use disorders (33%) were lower, likely because of overlapping symptoms. The overall classification accuracy (49%) was reasonable for a 7-class classifier.

Conclusions

A classification model was developed that could screen text for multiple mental health disorders. The screener resulted in an additional outcome score that may serve as input for a formal diagnostic interview and referral. This may lead to a more efficient and standardized intake process.
Machine Learning Decision Support for Detecting Lipohypertrophy With Bedside Ultrasound: Proof-of-Concept Study
Authors: Bandari Ela , Beuzen Tomas , Habashy Lara , Raza Javairia , Yang Xudong , Kapeluto Jordanna , Meneilly Graydon , Madden Kenneth
Published: April 2022

Background

The most common dermatological complication of insulin therapy is lipohypertrophy.

Objective

As a proof of concept, we built and tested an automated model using a convolutional neural network (CNN) to detect the presence of lipohypertrophy in ultrasound images.

Methods

Ultrasound images were obtained in a blinded fashion using a portable GE LOGIQ e machine with an L8-18I-D probe (5-18 MHz; GE Healthcare). The data were split into train, validation, and test splits of 70%, 15%, and 15%, respectively. Given the small size of the data set, image augmentation techniques were used to expand the size of the training set and improve the model’s generalizability. To compare the performance of the different architectures, the team considered the accuracy and recall of the models when tested on our test set.

Results

The DenseNet CNN architecture was found to have the highest accuracy (76%) and recall (76%) in detecting lipohypertrophy in ultrasound images compared to other CNN architectures. Additional work showed that the YOLOv5m object detection model could be used to help detect the approximate location of lipohypertrophy in ultrasound images identified as containing lipohypertrophy by the DenseNet CNN.

Conclusions

We were able to demonstrate the ability of machine learning approaches to automate the process of detecting and locating lipohypertrophy.
Perkembangan Wisata Halal di Jepang
Authors: Wahidati Lufi , Sarinastiti Eska Nia
Published: April 2022

Jepang adalah salah satu destinasi wisata yang menarik wisatawan Muslim dari Indonesia,Malaysia, dan negara-negara lain di Timur Tengah. Akhir-akhir ini, tingginya jumlah wisatawan Muslimmembuat Jepang menjadi sangat gencar mengembangkan fasilitas ramah Muslim untuk meningkatkanjumlah kunjungan wisatawan asing. Jepang adalah negara non-Muslim dengan penduduk mayoritasberagama Budha dan Shinto sehingga pemahaman masyarakatnya terhadap konsep halal dan wisatahalal tentunya sangat terbatas. Oleh karena itu, penelitian ini dilakukan untuk melihat apakah fasilitasramah Muslim yang mereka kembangkan sebagai bentuk omotenashi telah sesuai dengan standar wisatahalal yang diharapkan oleh wisatawan Muslim. Penelitian ini difokuskan pada karakteristik pelayananberbasis omotenashi, kebutuhan pengembangan wisata halal di Jepang, serta perkembangan fasilitasramah Muslim di Jepang. Data penelitian ini diambil dari jurnal dan website yang relevan dengan temapenelitian. Setelah dianalisis, dapat disimpulkan bahwa dibandingkan tahun-tahun sebelumnya, jumlahfasilitas ramah Muslim semakin meningkat. Dari enam kebutuhan (faith-based needs) wisatawan Muslim,empat di antaranya telah terpenuhi, yakni kebutuhan akan makanan halal, tempat ibadah, kamar kecildengan air, serta pelayanan rekreasional dengan privasi. Namun, masih terdapat beberapa masalah yangperlu diperhatikan oleh Jepang, yaitu 1) masih terdapat aktifitas non-halal di banyak restoran, 2) belumada pelayanan makan sahur bagi wisatawan yang berpuasa khususnya di bulan Ramadan, 3) terbatasnyajumlah restoran halal di kota kecil,4) belum terdapat badan sertifikasi halal yang ditunjuk secara resmioleh pemerintah Jepang, dan 5) terbatasnya jumlah musala yang m enyediakan fasilitas wudu.
Gagasan Optimalisasi Kerja Sama Pelestarian Arsip Puro Pakualaman
Authors: Rakhmawati Rina , Susanti Titi , Bramantya Arif Rahman
Published: April 2022

Arsip Puro Pakualaman merupakan salah satu kekayaan khazanah budaya Indonesia. Arsip puro menjadiidentitas keistimewaan Yogyakarta sekaligus rekam jejak peran Puro Pakualaman bagi Indonesia. Karakterkhas arsip Puro Pakualaman ada pada 2 (dua) aspek, yaitu aspek bahasa dan tulisan, dan aspek konten. Dalamaspek bahasa dan tulisan, arsip-arsip puro memiliki 3 (tiga) jenis, yaitu Belanda, Melayu, dan Arab Pegon.Pada aspek konten, arsip puro didominasi permasalahan pendidikan, sosial, dan hukum. Pengelolaan arsippuro dilakukan secara partnership atau kerja sama antar lembaga. Pada mulanya, partnership dilakukan antarapihak puro dengan Arsip Nasional RI. Setelah terbentuk lembaga kearsipan di tingkat daerah, pengelolaanarsip secara partnership dilakukan antara pihak puro dengan Pemerintah Daerah Istimewa Yogyakarta,dalam hal ini mengacu pada Badan Perpustakaan dan Arsip Daerah (BPAD) DIY. Penulis, dalam kajian ini,mendeskripsikan dinamika dan permasalahan yang ditemui dalam pengelolaan arsip Puro Pakualaman berbasispartnership. Penulis menggunakan 3 (tiga) metode penelitian dalam mengkaji permasalahan, yaitu: kajianpustaka, observasi partisipasi, dan wawancara tidak terstruktur. Simpulan dari kajian ini adalah optimalisasipartnership pengelolaan arsip puro, yaitu perluasan jejaring kerja sama dengan dunia Internasional dalambentuk peningkatan kualitas sarana dan prasarana pengelolaan dan kualitas sumber daya manusia pengelola.
A Comparative Study on Students’s English Listening Strategies In SV-UGM and CDTC
Authors: Nugraha Cisya Dewantara , Jia Liping
Published: April 2022

This study attempts to identify the use of listening strategies by technical and vocational college non-English majors, explore the relationship between listening strategies and listening proficiency and investigatethe difference between good and poor learners in the use of listening strategy to provide some pedagogicalimplications for teachers to guide the students to select, manage and adjust their listening strategies effectivelyin order to improve autonomous learning abilities, learning efficiency and English proficiency. The subjects inthe study are 75 first-year vocational college non-English majors from Chengdu Textile College and SV-UGM.First, all the participants took the model listening comprehension test. Second, the participants were given halfan hour to complete the questionnaire after finishing the model test. Thirdly, 10 students from both groups ofparticipants were interviewed individually for further information in the use of listening strategies and alsoexplore the relationship between the students’ listening strategy use. Finally, all the data was collected, andwas processed by SPSS22.0, then was analyzed as answers to the research questions. The result shows thatIndonesian students are more inclined to ask questions for explanation and verification. Furthermore, theyfocus on cooperative learning, which promotes a greater use of learning strategies than individual learning.However, Chinese learners are inclined to study English by themselves and get used to independent study. Theywould ask for help unless they meet some problems which cannot be solved by their own effort.
Desain dan Arsitektur untuk Memitigasi Fraud pada Organisasi Bisnis
Authors: Wulandari Rizky , Natasari Dina
Published: April 2022

Risiko merupakan hal yang akan selalu dihadapi baik secara individu maupun organisasi. Salah satu risikoyang akhir-akhir ini menjadi perhatian di Indonesia adalah adanya potensi terjadinya fraud yang bisa terjadi diberbagai organisasi. Diperlukan strategi dan desain pada setiap organisasi untuk dapat mencegah fraud tersebutsesuai dengan karakteristik masing-masing organisasi. Tujuan penelitian ini adalah merancang kerangkapencegahan fraud menggunakan pendekatan manajemen risiko dengan melihat pada risiko-risiko potensial yangterjadi pada suatu organisasi bisnis. Penelitian ini menggunakan jenis penelitian kualitataif dengan pendekatanstudi fenomenologi dan studi literatur. Penelitian ini mengacu pada kerangka yang telah ditetapkan pada standarmanajemen risiko. Oleh karena kerangka tersebut masih terlalu umum, maka diperlukan pengembangan untukmemenuhi tujuan pencegahan fraud. Standar manajemen risiko yang digunakan berdasarkan ISO 31000:2009.Desain dan Arsitektur mitigasi fraud terdiri dari prinsip antifraud, kerangka manajemen risiko fraud danproses manajemen risiko fraud. Hasil dari penelitian ini bahwa organisasi bisnis dapat menerapkan strategipencegahan fraud dengan menjalankan proses manajemen risiko fraud dengan tahapan (1)Memetakan sumber,area dan skema fraud yang sudah terjadi dan mungkin akan terjadi, (2)Mengidentifikasi risiko-risiko fraudberdasarkan area, (3) Memetakan risiko-risiko fraud tersebut pada skema-skema fraud sehingga terbentukrisk register, (4) Melakukan penilaian berkaitan dengan keterjadian dan dampak yang ditimbulkan sehinggaterbentuk peta risiko fraud, dan (5)Melakukan monitoring dan evaluasi.
Upah Minimum Provinsi (UMP) dan Penyerapan Tenaga Kerja di Pulau Jawa
Authors: Wihastuti Latri , Rahmatullah Henny
Published: April 2022

Permasalahan paling pokok dalam ketenagakerjaan Indonesia khususnya di Pulau Jawa terletak padatingkat kesempatan kerja. Beberapa faktor determinan secara teori yang dapat mempengaruhi penyerapantenaga kerja adalah pertumbuhan ekonomi dan upah perlu diuji di Pulau Jawa. Tujuan dari penelitian iniadalah untuk mengetahui pengaruh upah minimum (UMP) dan pertumbuhan ekonomi terhadap penyerapantenaga kerja di Pulau Jawa. Penelitian ini menggunakan pendekatan regresi data panel dengan 6 provinsi diPulau Jawa selama periode 2011-2016. Model terbaik yang dapat digunakan pada penelitian ini adalah modelfixed effect. Hasil penelitian ini menunjukkan bahwa UMP berpengaruh negatif signifikan terhadap penyerapantenaga kerja, sedangkan pertumbuhan ekonomi berpengaruh positif signifikan terhadap penyerapan tenagakerja. Penelitian ini menegaskan bahwa kebijakan UMP tetap menjadi kendala terciptanya keadilan di pasartenaga kerja karena menciptakan kekakuan harga. Hasil tersebut memberikan indikasi bagi pengambil kebijakanuntuk lebih berhati-hati dalam menentukan besaran UMP agar tidak mendestruksi tujuan utama pembangunanyaitu menciptakan kesejahteraan umum.
Makna Prosesi Upacara Adat Giling dan Suling di Pabrik Gula Madukismo Sebagai Atraksi Wisata Budaya
Authors: Pradana Cerry Surya
Published: April 2022

Pabrik gula, merupakan salah satu sarana untuk memperoleh pemasukan ekonomi bagi pemerintahan Belanda di Indonesia pada masa lalu. Di Yogyakarta, terdapat 17 (tujuhbelas) pabrik gula yang beroperasi hingga masa penjajahan Jepang. Saat ini hanya ada satu pabrik gula di Yogyakarta, yaitu Pabrik Gula Madukismo yang berada di bawah manajemen PT. Madubaru. Di samping pabrik gula, ada juga pabrik spiritus yang dijalankan. Sejak tahun 1955, pabrik ini memiliki sebuah ritual maupun upacara adat yang dimulai menjelang proses penggilingan dan penyulingan tebu di PT. Madubaru. Kegiatan ini dinamakan Upacara Giling dan Suling. Prosesi Upacara Giling dan Suling, memiliki rangkaian kegiatan yang cukup banyak (sekitar 15 kegiatan tradisi) sebelum akhirnya tebu digiling. Meskipun kegiatan ini telah berjalan lebih dari 50 tahun, akan tetapi saat ini alasan dan makna mengapa kegiatan tersebut dilaksanakan, semakin kabur. Penelitian ini bertujuan untuk mengungkap makna dibalik semua proses dalam Upacara Giling dan Suling yang diadakan oleh PT. Madubaru. Penelitian dilakukan secara kualitatif, dengan menitikberatkan pada hasil dari proses observasi secara langsung dan wawancara secara mendalam (in-depth interview). Di samping itu, studi pustaka yang sesuai dengan tema, juga menjadi acuan dalam penelitian ini, khususnya yang berkaitan dengan menguak makna simbolis maupun filosofis dari berbagai macam sarana upacara yang dilakukan di PT. Madubaru. Hasil analisis dibuat dengan merangkai hasil metode penelitian dengan teori-teori terkait, serta hasil observasi dan wawancara di lapangan. Hasil penelitian ini diharapkan mampu memberikan pemahaman kepada masyarakat mengenai tradisi Upacara Giling dan Suling, serta makna-makna yang terkandung di dalamnya. Sehingga prosesi tersebut tidak menjadi sekedar rutinitas dengan embel-embel ‘menjaga tradisi’, akan tetapi juga menjadi identitas yang dapat dinikmati oleh banyak pihak.
COVID-19, PERMINTAAN, DAN RATA-RATA UPAH TENAGA KERJA DI INDONESIA
Authors: Heldini Noorish
Published: April 2022

Penelitian ini bertujuan menganalisa dampak COVID-19 terhadap permintaan tenaga kerja dan rata-rata upah pekerja. Untuk menjawab tujuan tersebut, penelitian ini menggunakan data yang bersumber dari Badan Pusat Statistik. Berdasarkan data tersebut diketahui bahwa COVID-19 menyebabkan perubahan permintaan pekerja yang berbeda pada setiap sektor. Tidak semua sektor menunjukkan penurunan jumlah tenaga kerja, ada pula sektor yang justru meningkat jumlah tenaga kerjanya. Penurunan jumlah pekerja terjadi di sektor formal dan tingkat pengangguran meningkat pada seluruh golongan umur dan tingkat pendidikan. Hasil penelitian ini juga diketahui bahwa COVID-19 menyebabkan penurunan rata-rata upah pekeja di seluruh lapangan usaha. Rata-rata upah pekerja meningkat seiiring dengan meningkatnya pendidikan pekerja. Terakhir, rata-rata upah pekerja juga berbeda antar kelompok umur dimana pekerja berusia muda dibayar lebih rendah.