bluuebunny/crossref_metadata_2025 huggingface dataset:
Cold Plasma Synthesis and Testing of NiOX-Based Thin-Film Catalysts for CO2 Methanation
Authors: Smolarek Martyna , Kierzkowska-Pawlak Hanna , Kapica Ryszard , Fronczak Maciej , Sitarz Maciej , Leśniak Magdalena , Tyczkowski Jacek
Published: July 2021

An essential problem in managing CO2 and transforming it into methane as a useful fuel is the quest for adequately efficient and cheap catalysts. Another condition is imposed by the new designs of structured reactors, which require catalysts in the form of the thinnest possible films. The aim of this work was to produce Ni-based thin-film catalysts by the cold plasma deposition method (PECVD) from a volatile metal complex (Ni(CO)4) and to study their structure and catalytic properties in the CO2 methanation process. We tested three basic types of films: as-deposited, calcined in Ar, and calcined in air. The nanostructure and molecular structure of the films were investigated by electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The catalytic activity was evaluated in the methanation process (CO2 + H2), which was performed in a tubular reactor operating in the temperature range of 300–400 °C. The films calcined in air showed the highest activity in this process but behaved unstably. However, their regeneration by recalcination in air restored the initial catalytic activity. An important conclusion emerged from the obtained results, namely that the active phase in the tested films is Ni3+ (most likely in the form of Ni2O3), contrary to the common opinion that this phase is metallic Ni0. In our case, Ni0 quenches the catalytic activity.
Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of Equus asinus
Authors: Song Jun-Lin , Zhang Guo-Liang
Published: July 2021

(1) Background: Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in Equus asinus. This study investigated the biological effects of DON and ZEA exposure on Sertoli cells (SCs) of Equus asinus; (2) Methods: We administered 10 μM and 30 μM DON and ZEA to cells cultured in vitro; (3) Results: The results showed that 10 μM DON exposure remarkably changed pyroptosis-associated genes and that 30 μM ZEA exposure changed inflammation-associated genes in SCs. The mRNA expression of cancer-promoting genes was remarkably upregulated in the cells exposed to DON or 30 μM ZEA; in particular, DON and ZEA remarkably disturbed the expression of androgen and oestrogen secretion-related genes. Furthermore, quantitative RT-PCR, Western blot, and immunofluorescence analyses verified the different expression patterns of related genes in DON- and ZEA-exposed SCs; (4) Conclusions: Collectively, these results illustrated the impact of exposure to different toxins and concrete toxicity on the mRNA expression of SCs from Equus asinus in vitro.
An In Vitro System to Study the Effect of Subchondral Bone Health on Articular Cartilage Repair in Humans
Authors: Hopkins Timothy , Wright Karina T. , Kuiper Nicola J. , Roberts Sally , Jermin Paul , Gallacher Peter , Kuiper Jan Herman
Published: July 2021

Chondrocyte-based cartilage repair strategies, such as articular chondrocyte implantation, are widely used, but few studies addressed the communication between native subchondral bone cells and the transplanted chondrocytes. An indirect co-culture model was developed, representing a chondrocyte/scaffold-construct repair of a cartilage defect adjoining bone, where the bone could have varying degrees of degeneration. Human BM-MSCs were isolated from two areas of subchondral bone in each of five osteochondral tissue specimens from five patients undergoing knee arthroplasty. These two areas underlaid the macroscopically and histologically best and worst cartilage, representing early and late-stage OA, respectively. BM-MSCs were co-cultured with normal chondrocytes suspended in agarose, with the two cell types separated by a porous membrane. After 0, 7, 14 and 21 days, chondrocyte–agarose scaffolds were assessed by gene expression and biochemical analyses, and the abundance of selected proteins in conditioned media was assessed by ELISA. Co-culture with late-OA BM-MSCs resulted in a reduction in GAG deposition and a decreased expression of genes encoding matrix-specific proteins (COL2A1 and ACAN), compared to culturing with early OA BM-MSCs. The concentration of TGF-β1 was significantly higher in the early OA conditioned media. The results of this study have clinical implications for cartilage repair, suggesting that the health of the subchondral bone may influence the outcomes of chondrocyte-based repair strategies.
Melatonin, a Potential Therapeutic Agent for Preeclampsia, Reduces the Extrusion of Toxic Extracellular Vesicles from Preeclamptic Placentae
Authors: Tang Yunhui , Groom Katie , Chamley Larry , Chen Qi
Published: July 2021

Preeclampsia, characterised by maternal endothelial cell activation, is triggered by toxic factors, such as placental extracellular vesicles (EVs) from a dysfunctional placenta. The increased oxidative stress seen in the preeclamptic placenta links to endoplasmic reticulum (ER) stress. The ER regulates protein folding and trafficking. When the ER is stressed, proteins are misfolded, and misfolded proteins are toxic. Misfolded proteins can be exported from cells, via EVs which target to other cells where the misfolded proteins may also be toxic. Melatonin is a hormone and antioxidant produced by the pineal gland and placenta. Levels of melatonin are reduced in preeclampsia. In this study we investigated whether melatonin treatment can change the nature of placental EVs that are released from a preeclamptic placenta. EVs were collected from preeclamptic (n = 6) and normotensive (n = 6) placental explants cultured in the presence or absence of melatonin for 18 h. Misfolded proteins were measured using a fluorescent compound, Thioflavin-T (ThT). Endothelial cells were exposed to placental EVs overnight. Endothelial cell activation was measured by the quantification of cell-surface ICAM-1 using a cell-based ELISA. EVs from preeclamptic placentae carried significantly (p < 0.001) more misfolded proteins than normotensive controls. Incubating preeclamptic placental explants in the presence of melatonin (1 µM and 10 µM) significantly (p < 0.001) reduced the misfolded proteins carried by EVs. Culturing endothelial cells in the presence of preeclamptic EVs significantly increased the expression of ICAM-1. This increased ICAM-1 expression was significantly reduced when the endothelial cells were exposed to preeclamptic EVs cultured in the presence of melatonin. This study demonstrates that melatonin reduces the amount of misfolded proteins carried by EVs from preeclamptic placentae and reduces the ability of these EVs to activate endothelial cells. Our study provides further preclinical support for the use of melatonin as a treatment for preeclampsia.
Chemical Vapor Deposition of Graphene on Cu-Ni Alloys: The Impact of Carbon Solubility
Authors: Al-Hilfi Samir H. , Kinloch Ian A. , Derby Brian
Published: July 2021

Chemical vapour deposition (CVD) is the most promising graphene synthesis route for film and electronic applications but the growth mechanism is still not fully understood. Herein, we investigate the role of the solubility of carbon in the underlying growth substrate on the CVD growth of graphene. A range of Cu-Ni alloys compositions that cover the carbon (C) solubility range between low C solubility (pure Cu) and high C solubility (pure Ni) were used as the catalytic growth substrates. The CVD of graphene on Cu-Ni alloys showed a transition from bilayer graphene (BLG) to few-layer graphene (FLG) at a substrate Ni concentration of 45 wt.%, which was attributed to an increase in the bulk diffusion of C. The Cu-rich alloys had a high graphene coverage (BLG) at a fast-cooling rate (367 °C/min), while the Ni-rich alloys had a low coverage (FLG) under the same cooling condition. In contrast, at slow cooling rates (27 °C/min), the Cu-rich alloys had a low coverage of graphene (BLG) and the Ni-rich alloys had a high coverage of graphene (FLG). Glow discharge optical emission spectroscopy (GDOES) was used to profile the subsurface composition, particularly the C concentration, as a function of depth.
Effect of Micelle Encapsulation on Toxicity of CdSe/ZnS and Mn-Doped ZnSe Quantum Dots
Authors: Fan Qirui , Dehankar Abhilasha , Porter Thomas K. , Winter Jessica O.
Published: July 2021

The optical properties of quantum dots (QD) make them excellent candidates for bioimaging, biosensing, and therapeutic applications. However, conventional QDs are comprised of heavy metals (e.g., cadmium) that pose toxicity challenges in biological systems. Synthesising QDs without heavy metals or introducing thick surface coatings, e.g., by encapsulation in micelles, can reduce toxicity. Here, we examined the toxicity of micelle encapsulated tetrapod-shaped Mn-doped ZnSe QDs, comparing them to 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnSe QDs prepared by ligand exchange and commercial CdSe/ZnS QD systems that were either capped with MPA or encapsulated in micelles. HepG2 cell treatment with MPA-coated CdSe/ZnS QDs resulted in a dose-dependent reduction of viability (MTT assay, treatment at 0–25 μg/mL). Surprisingly, no reactive oxygen species (ROS) or apoptotic signaling was observed, despite evidence of apoptotic behavior in flow cytometry. CdSe/ZnS QD micelles showed minimal toxicity at doses up to 25 μg/mL, suggesting that thicker protective polymer layers reduce cytotoxicity. Despite their shape, neither MPA- nor micelle-coated Mn-doped ZnSe QDs displayed a statistically significant toxicity response over the doses investigated, suggesting these materials as good candidates for bioimaging applications.
Optical and Structural Characterization of Cd-Free Buffer Layers Fabricated by Chemical Bath Deposition
Authors: Vallejo William , Diaz-Uribe Carlos , Quiñones Cesar
Published: July 2021

Chemical bath deposition (CBD) is a suitable, inexpensive, and versatile synthesis technique to fabricate different semiconductors under soft conditions. In this study, we deposited Zn(O;OH)S thin films by the CBD method to analyze the effect of the number of thin film layers on structural and optical properties of buffer layers. Thin films were characterized by X-ray diffraction (XRD) and UV-Vis transmittance measurements. Furthermore, we simulated a species distribution diagram for Zn(O;OH)S film generation during the deposition process. The optical results showed that the number of layers determined the optical transmittance of buffer layers, and that the transmittance reduced from 90% (with one layer) to 50% (with four layers) at the visible range of the electromagnetic spectrum. The structural characterization indicated that the coatings were polycrystalline (α-ZnS and β-Zn(OH)2 to four layers). Our results suggest that Zn(O;OH)S thin films could be used as buffer layers to replace CdS thin films as an optical window in thin-film solar cells.
Changes in the Physical and Chemical Properties of Alder Wood in the Process of Thermal Treatment with Saturated Water Steam
Authors: Dudiak Michal , Dzurenda Ladislav
Published: July 2021

The paper presents changes in color and selected physico-chemical properties of alder (Alnus glutinosa) wood during the process of thermal treatment of the wood with a saturated steam-air mixture or saturated water steam in the temperature range t = 95–125 °C for τ = 3 to 12 h. During the process of thermal treatment of alder wood, the original light white-gray color changes depending on the temperature and time of modification to soft reddish-brown to dark brown color shades. Color changes of alder wood expressed in the form of the total color difference are in the range of values ∆E* = 2.7–31.7. Measurements of the density of thermally treated alder wood in the dry state indicate that due to the thermal treatment of alder wood, the density decreases by ρ ≤ 4.6% compared to the average density of native alder wood. Due to the hydrolysis of hemicelluloses, in the process of thermal treatment of wet alder wood, its acidity changes in the range of values: pH = 4.9 to 3.1. Analyzes of ATR-FTIR spectroscopy indicate changes in alder wood extractants and hemicellulose degradation. A decrease in unconjugated and an increase in conjugated carbonyls was observed at all temperatures of thermal modification of alder wood. Measurements indicate changes in the lignin of alder wood and the fact that as the temperature increases, the formation of new carbonyls increases, which is reflected in the change of the chromophoric system.
Temperature Rise Characteristics of Silicon Avalanche Photodiodes in Different External Capacitance Circuits Irradiated by Infrared Millisecond Pulse Laser
Authors: Chen Liang , Wei Zhi , Wang Di , Liu Hong-Xu , Jin Guang-Yong
Published: July 2021

We experimentally studied the interaction between a millisecond pulse laser and silicon avalanche photodiode (Si-APD) in an external capacitance circuit. The temperature rise law of Si-APD irradiated by a millisecond pulse laser under different external capacitance conditions was obtained. The results show that the surface temperature rise in a Si-APD is strongly dependent on the external capacitance. That is, the smaller the external capacitance, the smaller the surface temperature rise. The effect of the external capacitance on the surface temperature rise in a Si-APD was investigated for the first time in the field of laser damage. The research results have a certain practical significance for the damage and protection of mid-infrared detectors.
Microstructural Evolution of Large Cast Haynes 282 at Elevated Temperature
Authors: Yang Yujin
Published: July 2021

Haynes 282 has attracted attention for casting applications in AUSC power plants due to its good creep properties. However, the market is primarily comprised of wrought Haynes 282, while the cast version is not commercially available. In this study, the microstructure of a large traditional sand cast Haynes 282 was studied from as-cast condition to long-term heat-treated condition by combining experimental data and thermodynamic calculations. The microstructure of a large cast Haynes 282 includes γ, γ’, two types of MX, M23C6 and µ phases. After standard post heat treatment, µ phases were dissolved and precipitated as M6C. The equilibrium state was achieved after 266 h aging at 788 °C, after which γ’ particles began coarsening. These kept to a spherical morphology; the smallest misfit was found with the γ matrix. Once post heat treatment was finished, MX exhibited little morphology and compositional change during the long-term isothermal aging. Grain boundary is covered by discrete M23C6 and M6C precipitates and this morphology keeps stable during isothermal aging. No presence of the needle µ phase have been found at grain boundaries after 10,000 h aging at 788 °C. All these microstructural features indicated that cast Haynes 282 could have a high thermal stability and good creep properties.
Patterns in Nature—S-Layer Lattices of Bacterial and Archaeal Cells
Authors: Pum Dietmar , Breitwieser Andreas , Sleytr Uwe B.
Published: July 2021

Bacterial surface layers (S-layers) have been observed as the outermost cell envelope component in a wide range of bacteria and most archaea. S-layers are monomolecular lattices composed of a single protein or glycoprotein species and have either oblique, square or hexagonal lattice symmetry with unit cell dimensions ranging from 3 to 30 nm. They are generally 5 to 10 nm thick (up to 70 nm in archaea) and represent highly porous protein lattices (30–70% porosity) with pores of uniform size and morphology in the range of 2 to 8 nm. Since S-layers can be considered as one of the simplest protein lattices found in nature and the constituent units are probably the most abundantly expressed proteins on earth, it seems justified to briefly review the different S-layer lattice types, the need for lattice imperfections and the discussion of S-layers from the perspective of an isoporous protein network in the ultrafiltration region. Finally, basic research on S-layers laid the foundation for applications in biotechnology, synthetic biology, and biomimetics.
Unraveling the Epigenetic Role and Clinical Impact of Histone Deacetylases in Neoplasia
Authors: Goutas Dimitrios , Theocharis Stamatios , Tsourouflis Gerasimos
Published: July 2021

Histone deacetylases (HDACs) have long been implicated in tumorigenesis and tumor progression demonstrating their important participation in neoplasia. Therefore, numerous studies have been performed, highlighting the mechanism of HDACs action in tumor cells and demonstrating the potential role of HDAC inhibitors in the treatment of different cancer types. The outcome of these studies further delineated and strengthened the solid role that HDACs and epigenetic modifications exert in neoplasia. These results have spread promise regarding the potential use of HDACs as prospective therapeutic targets. Nevertheless, the clinical significance of HDAC expression and their use as biomarkers in cancer has not been extensively elucidated. The aim of our study is to emphasize the clinical significance of HDAC isoforms expression in different tumor types and the correlations noted between the clinicopathological parameters of tumors and patient outcomes. We further discuss the obstacles that the next generation HDAC inhibitors need to overcome, for them to become more potent.
Acute Mesenteric Vein Thrombosis in a Pregnant Patient at 10 Weeks Gestation: A Case Report
Authors: Chen Ying-Ying , Wu Sheng-Mao , Kosik Russell Oliver , Hsieh Yi-Chien , Wu Tzu-I , Chan Wing P.
Published: July 2021

Acute abdominal pain during pregnancy is challenging, both from a diagnostic and management perspective. A non-localized, persistent pain out of proportion to physical examination is a sign that advanced imaging may be necessary. Mesenteric venous thrombosis in a pregnant patient is extremely rare, but if diagnosis is delayed, can be potentially fatal to both the mother and the fetus. We present here a pregnant patient in the tenth week of gestation with classic clinical manifestations of mesenteric vein thrombosis and the corresponding findings on magnetic resonance imaging (MRI) and computed tomography (CT).
Clinical and Radiological Features Predicting Intervertebral Autofusion after Successful Antibiotic Therapy in Pyogenic Vertebral Osteomyelitis
Authors: Seong Insu , Kong Eunjung , Jeon Ikchan
Published: July 2021

Background: Pyogenic vertebral osteomyelitis (PVO) is a bacterial infection involving the intervertebral disc, vertebral body, and paravertebral soft tissues. Damaged intervertebral structure is a major cause of persistent back pain even after successful antibiotic therapy, which can be improved by achieving autofusion or via additional surgical fixation. In this study, we analyzed the clinical and radiological features predicting intervertebral autofusion after successful antibiotic therapy in lumbar PVO. Methods: This study was retrospectively conducted with 32 patients (20 men and 12 women) diagnosed with lumbar PVO that was completely cured with no recurrences after antibiotic therapy. They were divided into two groups with (group A, n = 18) and without (group B, n = 14) intervertebral autofusion at six-month follow-up. Differences in back pain, blood inflammatory markers, and radiological features of PVO on simultaneous 18F-fluorodeoxyglucose positron emission tomography/magnetic resonance imaging (18F-FDG-PET/MRI) of the intervertebral structure between the two groups were analyzed. Results: The mean duration of antibiotic therapy was 41.44 ± 14.21 (21–89) days. Group A showed a statistically higher erythrocyte sedimentation ratio (ESR; 59.28 ± 32.33 vs. 33.93 ± 18.76 mm/h, p = 0.014; normal range of ESR < 25), maximum standardized 18F-FDG uptake (SUVmax; 5.56 ± 1.86 vs. 3.98 ± 1.40, p = 0.013), and sustained extensive edematous changes on T2-weighted fat saturation (T2FS) MRI (p = 0.015) immediately after successful antibiotic therapy. However, no significant differences were observed in back pain, C-reactive protein, or the distribution of 18F-FDG uptake/contrast enhancement on 18F-FDG-PET/MRI (p > 0.05). Conclusions: Higher ESR and SUVmax of the intervertebral structure and sustained extensive edematous change on T2FS MRI immediately after successful antibiotic therapy are related with subsequent intervertebral autofusion, which should be carefully considered when assessing therapeutic response in PVO.
A Comparison of Lung Ultrasound and Computed Tomography in the Diagnosis of Patients with COVID-19: A Systematic Review and Meta-Analysis
Authors: Wang Mengshu , Luo Xufei , Wang Ling , Estill Janne , Lv Meng , Zhu Ying , Wang Qi , Xiao Xiaojuan , Song Yang , Lee Myeong Soo , Ahn Hyeong Sik , Lei Junqiang , Tian Jinhui
Published: July 2021

Background Lung ultrasound (LUS) and computed tomography (CT) can both be used for diagnosis of interstitial pneumonia caused by coronavirus disease 2019 (COVID-19), but the agreement between LUS and CT is unknown. Purpose to compare the agreement of LUS and CT in the diagnosis of interstitial pneumonia caused by COVID-19. Materials and Methods We searched PubMed, Cochrane library, Embase, Chinese Biomedicine Literature, and WHO COVID-19 databases to identify studies that compared LUS with CT in the diagnosis of interstitial pneumonia caused by COVID-19. We calculated the pooled overall, positive and negative percent agreements, diagnostic odds ratio (DOR) and the area under the standard receiver operating curve (SROC) for LUS in the diagnosis of COVID-19 compared with CT. Results We identified 1896 records, of which nine studies involving 531 patients were finally included. The pooled overall, positive and negative percentage agreements of LUS for the diagnosis of interstitial pneumonia caused by COVID-19 compared with CT were 81% (95% confidence interval [CI] 43–99%), 96% (95% CI, 80–99%, I2 = 92.15%) and 80% (95%CI, 60–92%, I2 = 92.85%), respectively. DOR was 37.41 (95% CI, 9.43–148.49, I2 = 63.9%), and the area under the SROC curve was 0.94 (95% CI, 0.92–0.96). The quality of evidence for both specificity and sensitivity was low because of heterogeneity and risk of bias. Conclusion The level of diagnostic agreement between LUS and CT in the diagnosis of interstitial pneumonia caused by COVID-19 is high. LUS can be therefore considered as an equally accurate alternative for CT in situations where molecular tests are not available.
Prioritizing English-Medium Instruction Teachers’ Needs for Faculty Development and Institutional Support: A Best–Worst Scaling Approach
Authors: Uehara Takuro , Kojima Naoko
Published: July 2021

This study explored the prioritization of English-medium instruction (EMI) teachers’ needs for faculty development and institutional support by applying a best–worst scaling approach to an EMI program in Japan. This prioritization is important as EMI programs need management under various constraints (e.g., time, budget, and teachers). This study also investigated how teachers’ needs for institutional support differ by English language competence and EMI teaching experience and their relationship with EMI programs (e.g., full-time or adjunct). Questionnaire surveys administered to 38 EMI teachers revealed that, overall, faculty development (FD) program menus training teaching styles, speaking skills, communication skills, and respecting the diversity of students should be prioritized such that it varies depending on the teachers’ English language competence levels but not their teaching experience. Irrespective of their relationship with EMI programs, the recognition and appreciation of their burdens, efforts, and contributions is most needed. There are noticeable differences based on their position over the necessity of pedagogical guidelines, teaching load, and economic incentive.
Preferences and Scores of Different Types of Exams during COVID-19 Pandemic in Faculty of Veterinary Medicine in Spain: A Cross-Sectional Study of Paper and E-exams
Authors: Marín García Pablo-Jesús , Arnau-Bonachera Alberto , Llobat Lola
Published: July 2021

The World Health Organization (WHO) officially declared the novel coronavirus (COVID-19) as a pandemic on 11 March 2020, and educational institutions have had to modify most of their activities (face-to-face activities were suspended). This situation forced academic institutions to modify the evaluation format of students. The use of proctoring systems quickly became widespread, although some controversies arose. The two main discussions regarding these systems are the integrity of the assessment and the capacity of the students to adapt to this new assessment method, without changes in theirs scores. To elucidate two controversies, we have analyzed the preferences and the scores obtained from a trial of 660 scores from 332 students of the third grade of Veterinary Medicine. The experiment involved three modalities of exam: an online format from home using the Respondus Lockdown Browser system (Modality 1), online in person using the Respondus Lockdown Browser system with the supervision of a teacher (Modality 2), or paper format in person with the supervision of a teacher (Modality 3). The results obtained showed that the students preferred Modality 1 (online at home with Respondus Lockdown Browser system). No statistical differences between the scores obtained by students were found between the three modalities analyzed. The proctoring system is a good method to adjudicate exams in higher education institutions, and the scores of students are similar to those obtained through traditional evaluation and control systems.
Deformable Bowtie Antenna Realized by 4D Printing
Authors: Wu Lei , Huang Jiawei , Zhai Minglong , Sun Bing , Chang Hudong , Huang Sen , Liu Honggang
Published: July 2021

4D printing is utilized to fabricate of thermo-deformable bow-tie antenna to fulfill some special applications with limited space or changing antenna property. In this paper, 4D printing is used to manufacture nylon and carbon fiber laminated composite material. The bow-tie antenna is installed on the surface of the composite material, and the carbon fiber is energized and heated, which causes thermal deformation of the substrate to reconfigure the antenna feature. The deformation mechanism of the composite material is explained, the characteristics of the thermally deformed bow-tie antenna with power applied to carbon fiber are analyzed. The results show that the energized carbon fiber heats up, causing the structure to stretch to a flat, with a maximum gain of 2.37 dBi and the −10 dB bandwidth being 4.28–4.64 GHz and 5.16–5.52 GHz, and the half-power beamwidth is greater than 60°. The structure bends at a 30° angle with a maximum gain of 3.58 dBi in the absence of external power, delivering a −10 dB bandwidth range of 4.12–5.6 GHz and a half-power beamwidth close to 45°. The customization of antenna radiation patterns and antenna gain can be readily tuned with power control.
High-Speed Railway
Authors: Watson Inara
Published: July 2021

Union Internationale des Chemins (UIC) defines the high-speed railway (HSR) as a high-speed railway system that contains the infrastructure and the rolling stock. The infrastructure can be newly built dedicated lines enabled for trains to travel with speed above 250 km/h or upgraded conventional lines with a speed up to 200 or even 220 km/h. HSR requires specially built trains with increased power to weight ratio and must have an in-cab signalling system as traditional signalling systems are incapable of above 200 km/h.
Complete Chloroplast Genome Sequence of Fortunella venosa (Champ. ex Benth.) C.C.Huang (Rutaceae): Comparative Analysis, Phylogenetic Relationships, and Robust Support for Its Status as an Independent Species
Authors: Wang Ting , Kuang Ren-Ping , Wang Xiao-Hui , Liang Xiao-Li , Wanga Vincent Okelo , Liu Ke-Ming , Cai Xiu-Zhen , Hu Guang-Wan
Published: July 2021

Fortunella venosa (Rutaceae) is an endangered species endemic to China and its taxonomic status has been controversial. The genus Fortunella contains a variety of important economic plants with high value in food, medicine, and ornamental. However, the placement of Genus Fortunella into Genus Citrus has led to controversy on its taxonomy and Systematics. In this present research, the Chloroplast genome of F. venosa was sequenced using the second-generation sequencing, and its structure and phylogenetic relationship analyzed. The results showed that the Chloroplast genome size of F. venosa was 160,265 bp, with a typical angiosperm four-part ring structure containing a large single copy region (LSC) (87,597 bp), a small single copy region (SSC) (18,732 bp), and a pair of inverted repeat regions (IRa\IRb) (26,968 bp each). There are 134 predicted genes in Chloroplast genome, including 89 protein-coding genes, 8 rRNAs, and 37 tRNAs. The GC-content of the whole Chloroplast genome was 43%, with the IR regions having a higher GC content than the LSC and the SSC regions. There were no rearrangements present in the Chloroplast genome; however, the IR regions showed obvious contraction and expansion. A total of 108 simple sequence repeats (SSRs) were present in the entire chloroplast genome and the nucleotide polymorphism was high in LSC and SSC. In addition, there is a preference for codon usage with the non-coding regions being more conserved than the coding regions. Phylogenetic analysis showed that species of Fortunella are nested in the genus of Citrus and the independent species status of F. venosa is supported robustly, which is significantly different from F. japonica. These findings will help in the development of DNA barcodes that can be useful in the study of the systematics and evolution of the genus Fortunella and the family Rutaceae.
Apple Fruit (Malus domestica Borkh.) Metabolic Response to Infestation by Invasive Brown Marmorated Stink Bug (Halyomorpha halys Stal.)
Authors: Zamljen Tilen , Medič Aljaž , Veberič Robert , Hudina Metka , Štampar Franci , Slatnar Ana
Published: July 2021

The brown marmorated stink bug (BMSB; Halyomorpha halys Stal.) has become a significant pest in Slovenia, especially in apple, pear, peach, and cherry orchards. In our study, apple fruit of the apple cultivar ‘Red Pinova’ were evaluated for visual injury and sugar, organic acid, and phenolic contents. The chemical composition of the area around the puncture wound, the uninjured part of the infested apple, was compared to, as a control, only uninjured apples. There was a significant response of the apple around the puncture wound, resulting in an 11.9 g/kg FW higher total sugar content, a 1.4 g/kg FW lower total organic acid content, and an 11.9 g/kg FW higher total phenolic content compared with control apples. A strong phenolic response in the puncture wound area, with high flavanol and hydroxycinnamic acid contents, with increases of 118% and 237%, respectively, compared with control apples, was detected. The brown marmorated stink bug induces a strong phenolic response in the injured area of the apple. The results of this study illustrated how apple fruit responds to the BMSB injury, not only sensorily (visual injury, odor), but also chemically in the form of metabolic responses.
The Impact of Higher Education Expansion on the Educational Wage Premium in Taiwan: 1985 to 2015
Authors: Chen Chien-Liang , Chen Lin-Chuan
Published: July 2021

This research investigates the impact of higher education expansion on the educational wage premium from a long-term perspective in Taiwan. By using 1985 to 2015 Manpower Utilization Survey (MUS) data with the difference-in-difference-in-differences model (DDD), this study analyzes the change of the wage premium of university educated versus lower-than-university educated counterparts across the expansion of higher education since 1995. The number of universities in Taiwan tripled between 1995 and 2005, from 50 to more than 150, with upgrading of about 100 technology colleges and vocational schools additionally. Dramatic expansion of universities as well as the number of university graduates will shrink the university wage premium for the young generation who entered into the labor market after year 2000, but the older generation will be less affected. The empirical results show that the wages premium of university graduates of the younger generation is 12% to 21% lower than their older generation counterparts due to the higher-education expansion.
Efficient Interactive Tactile Maps: A Semi-Automated Workflow Using the TouchIt3D Technology and OpenStreetMap Data
Authors: Barvir Radek , Vondrakova Alena , Brus Jan
Published: July 2021

Despite the growing efficiency of the map-design process in general, tactile mapping has remained peripheral to mainstream cartography. For a specific group of people with visual impairment, however, tactile maps are the only effective way to obtain a complex idea about the geospatial distribution of the surrounding world. As there are numerous specifics in creating these 3D maps and only a limited group of users, tactile products have usually been either very simple creations or, on the other hand, difficult and expensive to produce. Modern trends and progress in the availability of new technologies (e.g., 3D printing) bring new possibilities for keeping tactile map production both effective and up to date. Therefore, this paper aims to present a methodology to apply the TouchIt3D technology to link 3D-printed multi-material tactile maps with a mobile device. Utilizing this solution resulted in a set of interactive tactile maps following current trends of inclusive education. Using OpenStreetMap data together with a semi-automated workflow significantly lowered expenses compared to antecedent maps with similar functionality. A semi-automated workflow was designed, focusing on three use cases of independent movement: walking, using public transport, and tourism.
Photobiological Effects on Ice Algae of a Rapid Whole-Fjord Loss of Snow Cover during Spring Growth in Kangerlussuaq, a West Greenland Fjord
Authors: Sorrell Brian K. , Hawes Ian , Stratmann Tanja , Lund-Hansen Lars Chresten
Published: July 2021

Snow cover on sea ice is the most important factor controlling light availability for sea ice algae, but it is predicted by climate models to become more variable and stochastic. Here, we document effects of a sudden, complete loss of the entire snow cover on first-year sea ice at Kangerlussuaq Fjord, West Greenland, due to a natural Föhn wind event that caused a ca. 17 °C air temperature increase over 36 h. We applied Imaging-PAM fluorometry to examine effects of snow cover on algal distribution and photobiology and observed a rapid decrease in algal biomass associated with loss of the skeletal ice crystal layer on the underside of the ice that had supported most of the visible algae. Furthermore, the remaining algae were photobiologically stressed, as seen in a significant decrease in the dark-acclimated fluorescence yield (ΦPSII_max) from 0.55 before snow loss to 0.41 after. However, recovery in the dark suggested that non-photosynthetic quenching was successfully dissipating excess energy in the community and that there was little photodamage. An observed decrease in the photosynthetic efficiency α from 0.22 to 0.16 µmol é m−2 s−1 is therefore likely to be due to photoacclimation and the change in community composition. Centric diatoms and flagellates were the main taxa lost in the snow loss event, whereas the sea ice specialist Nitzschia frigida increased in numbers. These observations are similar to those seen in artificial snow-clearing experiments and consistent with snow clearing being a useful approach for investigating the complex interactions between snow cover, irradiance fluctuations, and ice algal performance.
Testing Practices for Fungal Respiratory Infections and SARS-CoV-2 among Infectious Disease Specialists, United States
Authors: Benedict Kaitlin , Williams Samantha , Beekmann Susan E. , Polgreen Philip M. , Jackson Brendan R. , Toda Mitsuru
Published: July 2021

In an online poll, 174 infectious disease physicians reported that testing frequencies for coccidioidomycosis, histoplasmosis, blastomycosis, and cryptococcosis were similar before and during the COVID-19 pandemic, indicating that these physicians remain alert for these fungal infections and were generally not concerned about the possibility of under-detection.
The Diplodia Tip Blight Pathogen Sphaeropsis sapinea Is the Most Common Fungus in Scots Pines’ Mycobiome, Irrespective of Health Status—A Case Study from Germany
Authors: Blumenstein Kathrin , Bußkamp Johanna , Langer Gitta Jutta , Langer Ewald Johannes , Terhonen Eeva
Published: July 2021

The opportunistic pathogen Sphaeropsis sapinea (≡Diplodia sapinea) is one of the most severe pathogens in Scots pine, causing the disease Diplodia tip blight on coniferous tree species. Disease symptoms become visible when trees are weakened by stress. Sphaeropsis sapinea has an endophytic mode in its lifecycle, making it difficult to detect before disease outbreaks. This study aims to record how S. sapinea accumulates in trees of different health status and, simultaneously, monitor seasonal and age-related fluctuations in the mycobiome. We compared the mycobiome of healthy and diseased Scots pines. Twigs were sampled in June and September 2018, and filamentous fungi were isolated. The mycobiome was analyzed by high-throughput sequencing (HTS) of the ITS2 region. A PERMANOVA analysis confirmed that the mycobiome community composition significantly differed between growth years (p < 0.001) and sampling time (p < 0.001) but not between healthy and diseased trees. Sphaeropsis sapinea was the most common endophyte isolated and the second most common in the HTS data. The fungus was highly abundant in symptomless (healthy) trees, presenting in its endophytic mode. Our results highlight the ability of S. sapinea to accumulate unnoticed as an endophyte in healthy trees before the disease breaks out, representing a sudden threat to Scots pines in the future, especially with increasing drought conditions experienced by pines.
Personalized Medicine Approach in Treating Parkinson’s Disease, Using Oral Administration of Levodopa/Carbidopa Microtablets in Clinical Practice
Authors: Grétarsdóttir Helga María , Widman Erik , Johansson Anders , Nyholm Dag
Published: July 2021

Background: The most effective symptomatic treatment in Parkinson’s disease (PD) is levodopa in standard doses. However, as the disease progresses, there may be a need for a more personalized approach and fine tuning, in accordance with the patients’ needs. This study aims to evaluate the individual experience of levodopa/carbidopa 5/1.25 mg microtablets (LC-5) in clinical practice with respect to efficacy, tolerability, and usability. The method used was as follows: patients answered a questionnaire concerning the effect and usability of LC-5, and their medical records were reviewed. Regarding results, thirty-five survey responses were obtained, and 29 patients’ medical records were reviewed. The LC-5 dose dispenser usability was generally rated positively and facilitated medication adherence. The majority (85%) of patients reported symptom improvement while using LC-5, compared with previous standard treatments. These results suggest that LC-5 therapy is generally well-tolerated, with favorable patient-reported efficacy and user friendliness, as well as the possibility for an individualized, fine-tuned PD treatment. Further studies with a prospective design and larger study population are needed to confirm the results.
Electronic Cigarettes and Asthma: What Do We Know So Far?
Authors: Kotoulas Serafeim-Chrysovalantis , Katsaounou Paraskevi , Riha Renata , Grigoriou Ioanna , Papakosta Despoina , Spyratos Dionysios , Porpodis Konstantinos , Domvri Kalliopi , Pataka Athanasia
Published: July 2021

Electronic cigarettes (EC) are a novel product, marketed as an alternative to tobacco cigarette. Its effects on human health have not been investigated widely yet, especially in specific populations such as patients with asthma. With this review, we use the existing literature in order to answer four crucial questions concerning: (1) ECs’ role in the pathogenesis of asthma; (2) ECs’ effects on lung function and airway inflammation in patients with asthma; (3) ECs’ effects on asthma clinical characteristics in asthmatics who use it regularly; and (4) ECs’ effectiveness as a smoking cessation tool in these patients. Evidence suggests that many EC compounds might contribute to the pathogenesis of asthma. Lung function seems to deteriorate by the use of EC in this population, while airway inflammation alters, with the aggravation of T-helper-type-2 (Th2) inflammation being the most prominent but not the exclusive effect. EC also seems to worsen asthma symptoms and the rate and severity of exacerbations in asthmatics who are current vapers, whilst evidence suggests that its effectiveness as a smoking cessation tool might be limited. Asthmatic patients should avoid using EC.
Risk-Stratified Approach to Breast Cancer Screening in Canada: Women’s Knowledge of the Legislative Context and Concerns about Discrimination from Genetic and Other Predictive Health Data
Authors: Alarie Samuel , Hagan Julie , Dalpé Gratien , Faraji Sina , Mbuya-Bienge Cynthia , Nabi Hermann , Pashayan Nora , Brooks Jennifer , Dorval Michel , Chiquette Jocelyne , Eloy Laurence , Turgeon Annie , Lambert-Côté Laurence , Paquette Jean-Sébastien , Walker Meghan , Lapointe Julie , Granados Moreno Palmira , Blackmore Kristina , Wolfson Michael , Broeders Mireille ,   The PERSPECTIVE I&I Study Group, Knoppers Bartha , Chiarelli Anna , Simard Jacques , Joly Yann
Published: July 2021

The success of risk-stratified approaches in improving population-based breast cancer screening programs depends in no small part on women’s buy-in. Fear of genetic discrimination (GD) could be a potential barrier to genetic testing uptake as part of risk assessment. Thus, the objective of this study was twofold. First, to evaluate Canadian women’s knowledge of the legislative context governing GD. Second, to assess their concerns about the possible use of breast cancer risk levels by insurance companies or employers. We use a cross-sectional survey of 4293 (age: 30–69) women, conducted in four Canadian provinces (Alberta, British Colombia, Ontario and Québec). Canadian women’s knowledge of the regulatory framework for GD is relatively limited, with some gaps and misconceptions noted. About a third (34.7%) of the participants had a lot of concerns about the use of their health information by employers or insurers; another third had some concerns (31.9%), while 20% had no concerns. There is a need to further educate and inform the Canadian public about GD and the legal protections that exist to prevent it. Enhanced knowledge could facilitate the implementation and uptake of risk prediction informed by genetic factors, such as the risk-stratified approach to breast cancer screening that includes risk levels.
Sweat Chloride Testing and Nasal Potential Difference (NPD) Are Primary Outcome Parameters in Treatment with Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Modulators
Authors: Sermet-Gaudelus Isabelle , Nguyen-Khoa Thao , Hatton Aurélie , Hayes Kate , Pranke Iwona
Published: July 2021

With the advent of CFTR modulators, surrogate outcome parameters that accurately quantify the improvement in CFTR activity are needed. In vivo biomarkers that reflect CFTR ion transport and can serve as outcomes in the treatment of CFTR modulators are the sweat Cl− test (SCT), the nasal potential difference (NPD) measurement or the intestinal current measurement (ICM). This review focus on the SCT and NPD. The SCT displays a low intra-patient variability in contrast to the NPD. It has been used extensively as a biomarker of CFTR function in clinical trials of CFTR modulator therapies and provides evidence for change in the short term. The level of functional rescue in the NPD increases up to 40% of normal CFTR in patients with a Gly551Asp treated with ivacaftor monotherapy, while in F508del homozygous patients treated with ivacaftor-lumacaftor, activity increased on average up to ~20% of normal activity. While both tests provide evidence of the effect on CFTR activity, they cannot be used at an individual level to predict the response to any CFTR modulators. Nevertheless, their rapid modification, reflecting electrophysiological properties, highlight their potential use in proof-of-concept studies for CFTR modulators.
Preliminary Pharmacogenetic Study to Explore Putative Dopaminergic Mechanisms of Antidepressant Action
Authors: Ochi Taichi , Vyalova Natalya M. , Losenkov Innokentiy S. , Paderina Diana Z. , Pozhidaev Ivan V. , Loonen Anton J. M. , Simutkin German G. , Bokhan Nikolay A. , Wilffert Bob , Ivanova Svetlana A.
Published: July 2021

Background: There is sufficient evidence that interference of dopaminergic neurotransmission contributes to the therapeutic effects of antidepressants in unipolar and bipolar depression. Methods: Hamilton depression rating scale (HAMD 17) scores of 163 at least moderately ill patients with major depressive disorders were used to establish treatment response. HAMD 17 score status was measured before initiation, after two weeks, and after four weeks of treatment with various antidepressants. The possible association between response and genotype in a total of 14 variants of dopamine neurotransmission-related proteins was investigated. Results: DRD4 rs11246226 CA heterozygous patients were found with a greater improvement of HAMD 17 score when compared to homozygous C patients during 0–2 weeks and 0–4 weeks. Patients with MAOB rs1799836 heterozygous GA and homozygous A also demonstrated improved scores during 2–4 weeks and 0–4 weeks. Conclusions: The results are preliminary due to the limited population size and the small number of variants. Further research into the involvement of habenular dopamine D4 receptors in the antidepressant response is desirable.
Impact Dynamics Analysis of Mobile Mechanical Systems
Authors: Dumitru Sorin , Constantin Andra , Copilusi Cristian , Dumitru Nicolae
Published: July 2021

The current paper focuses on the impact phenomenon analysis, in the case of multi-body mechanical systems undergoing fast motion, due to the presence of some manufacturing and mounting errors or due to some accident during the transport mechanical systems. Thus, the impact phenomenon was analyzed in two cases, the first one consisting of a two bodies, namely, a free-fall body brought in contact with the other considered fixed in space and the second case, which is a complex one, when the analyzed bodies are components of a multi-body mechanical system. The research main objective is to analyze the impact generated between the two bodies through three methods, i.e., the analytical method, a virtual prototyping method accomplished with MSC Adams software and a method based on finite element analysis with Ansys and Abaqus software. A dynamic model of the impact force was developed, which allows to make a comparison of the numerical results obtained through the abovementioned methods. As a multi-body mechanical system, it was considered a mechanism from an internal combustion engine from which the radial clearance between the piston bolt and connecting rod head of the considered mechanism was analyzed.
A New Class of Plane Curves with Arc Length Parametrization and Its Application to Linear Analysis of Curved Beams
Authors: Maksimović Snježana , Borković Aleksandar
Published: July 2021

The objective of this paper is to define one class of plane curves with arc-length parametrization. To accomplish this, we constructed a novel class of special polynomials and special functions. These functions form a basis of L2(R) space and some of their interesting properties are discussed. The developed curves are used for the linear static analysis of curved Bernoulli–Euler beam. Due to the parametrization with arc length, the exact analytical solution can be obtained. These closed-form solutions serve as the benchmark results for the development of numerical procedures. One such example is provided in this paper.