bluuebunny/crossref_metadata_2025 huggingface dataset:
Structure, Z′ = 2 Crystal Packing Features of 3-(2-Chlorobenzylidene)-5-(p-tolyl)furan-2(3H)-one
Authors: Grinev Vyacheslav S. , Mayorova Oksana A. , Anis’kova Tatyana V. , Tikhomolova Alexandra S. , Yegorova Alevtina Yu.
Published: April 2021

3-(2-Chlorobenzylidene)-5-(p-tolyl)furan-2(3H)-one (1), C18H13ClO2, crystallizes with Z = 8 and Z′ = 2, and the structure at 100 K has orthorhombic (Pna21) symmetry. Each kind of molecule takes part in π–π stacking interactions to form infinite chains parallel to the c axis. We believe that the existence of two forms can be explained by the probable rotation around a single C–C bond. The quantum chemical modeling reveals that these molecules are almost equivalent energetically, and they can be described as the two most stable conformers (rotamers) with a minor rotational barrier of about 0.67 kcal/mol.
Novel Ring Systems: Spiro[Cycloalkane] Derivatives of Triazolo- and Tetrazolo-Pyridazines
Authors: Sepsey Für Csilla Sepsey , Riszter Gergő , SzigetvárI Áron , Dékány Miklós , Keglevich György , HazaI László , BölcskeI Hedvig
Published: April 2021

In orderto synthesize new pyridazine derivatives anellated with different nitrogen heterocyclic moieties, spiro[cycloalkane]pyridazinones were transformed into the corresponding thioxo derivatives via a reaction with phosphorus pentasulfide. The reaction of the formed 2,3-diazaspiro[5.5] undec-3-ene-1-thiones with hydrazine provided the corresponding 1-hydrazono-2,3-diazaspiro[5.5] undec-3-ene, whose diazotization led to the desired spiro[cyclohexane-1,8′-tetrazolo[1,5-b]pyridazines. The reaction of dihydropyridazinethiones with benzhydrazide afforded the corresponding 7H-spiro[[1,2,4]triazolo[4,3-b]pyridazin-8,1′-cyclohexanes]. As a result of our work, seven new pyridazinethione intermediates were prepared, which served as starting materials for the synthesis of two kinds of new ring systems: tetrazolo-pyridazines and triazolo-pyridazines. The six new annulated derivatives were characterized by physicochemical parameters. The new N-heterocycles are valuable members of the large family of pyridazines.
Chemical Hemisynthesis of Sulfated Cyanidin-3-O-Glucoside and Cyanidin Metabolites
Authors: Straßmann Sarah , Passon Maike , Schieber Andreas
Published: April 2021

The metabolism of anthocyanins in humans is still not fully understood, which is partly due to the lack of reference compounds. It is known that sulfation is one way of the complex phase II biotransformation mechanism. Therefore, cyanidin-3-O-glucoside and the cyanidin aglycone were chemically converted to their sulfates by reaction with sulfur trioxide-N-triethylamine complex in dimethylformamide. The reaction products were characterized by UHPLC coupled to linear ion trap and IMS-QTOF mass spectrometry. Based on MS data, retention times, and UV-Vis spectra, the compounds could tentatively be assigned to A-, C-, or B-ring sulfates. Analysis of urine samples from two volunteers after ingestion of commercial blackberry nectar demonstrated the presence of two sulfated derivatives of the cyanidin aglycone and one sulfated derivative of the cyanidin-3-O-glucoside. It was found that both the A ring and the B ring are sulfated by human enzymes. This study marks an important step toward a better understanding of anthocyanin metabolism.
Is the Antidepressant Activity of Selective Serotonin Reuptake Inhibitors Mediated by Nicotinic Acetylcholine Receptors?
Authors: Arias Hugo R. , Targowska-Duda Katarzyna M. , García-Colunga Jesús , Ortells Marcelo O.
Published: April 2021

It is generally assumed that selective serotonin reuptake inhibitors (SSRIs) induce antidepressant activity by inhibiting serotonin (5-HT) reuptake transporters, thus elevating synaptic 5-HT levels and, finally, ameliorates depression symptoms. New evidence indicates that SSRIs may also modulate other neurotransmitter systems by inhibiting neuronal nicotinic acetylcholine receptors (nAChRs), which are recognized as important in mood regulation. There is a clear and strong association between major depression and smoking, where depressed patients smoke twice as much as the normal population. However, SSRIs are not efficient for smoking cessation therapy. In patients with major depressive disorder, there is a lower availability of functional nAChRs, although their amount is not altered, which is possibly caused by higher endogenous ACh levels, which consequently induce nAChR desensitization. Other neurotransmitter systems have also emerged as possible targets for SSRIs. Studies on dorsal raphe nucleus serotoninergic neurons support the concept that SSRI-induced nAChR inhibition decreases the glutamatergic hyperstimulation observed in stress conditions, which compensates the excessive 5-HT overflow in these neurons and, consequently, ameliorates depression symptoms. At the molecular level, SSRIs inhibit different nAChR subtypes by noncompetitive mechanisms, including ion channel blockade and induction of receptor desensitization, whereas α9α10 nAChRs, which are peripherally expressed and not directly involved in depression, are inhibited by competitive mechanisms. According to the functional and structural results, SSRIs bind within the nAChR ion channel at high-affinity sites that are spread out between serine and valine rings. In conclusion, SSRI-induced inhibition of a variety of nAChRs expressed in different neurotransmitter systems widens the complexity by which these antidepressants may act clinically.
Morin Induces Melanogenesis via Activation of MAPK Signaling Pathways in B16F10 Mouse Melanoma Cells
Authors: Shin SeoYeon , Ko JaeYeon , Kim MinJeong , Song Nuri , Park KyungMok
Published: April 2021

Morin is a well-known flavonoid, and has been reported to have various properties, such as anti-cell death, antioxidant, and anti-inflammatory properties. Although studies on the biochemical and biological actions of morin have been reported, the melanin biosynthesis effects and molecular mechanisms are unknown. In this study, we first found that morin has the effect of enhancing melanin biosynthesis in B16F10 mouse melanoma cells, and analyzed the molecular mechanism. In this study, we examined the effects of morin on the melanin contents and tyrosinase activity, as well as the protein expression levels of the melanogenic enzymes TRP-1, TRP-2, and microphtalmia-associated transcription factor (MITF) in B16F10 mouse melanoma cells. Morin showed no cytotoxicity in the concentration range of 5–100 μM, and significantly increased the intracellular tyrosinase activity and melanin contents. In mechanism analysis, morin increased the protein expression of TRP-1, TRP-2, and MITF associated with melanogenesis. Furthermore, morin increased phosphorylated ERK and p38 at the early time, and decreased phosphorylated ERK after 12 h. The results suggest that morin enhances melanin synthesis through the MAPK signaling pathways in B16F10 mouse melanoma cells.
Antioxidant and Pro-Oxidant Capacities as Mechanisms of Photoprotection of Olive Polyphenols on UVA-Damaged Human Keratinocytes
Authors: Lecci Raffaella Marina , D’Antuono Isabella , Cardinali Angela , Garbetta Antonella , Linsalata Vito , Logrieco Antonio F. , Leone Antonella
Published: April 2021

A wide variety of polyphenols are reported to have considerable antioxidant and skin photoprotective effects, although the mechanisms of action are not fully known. Environmentally friendly and inexpensive sources of natural bioactive compounds, such as olive mill wastewater (OMWW), the by-product of olive-oil processing, can be considered an economic source of bioactive polyphenols, with a range of biological activities, useful as chemotherapeutic or cosmeceutical agents. Green strategies, such as the process based on membrane technologies, allow to recover active polyphenols from this complex matrix. This study aims to evaluate the antioxidant, pro-oxidant, and photoprotective effects, including the underlying action mechanism(s), of the ultra-filtered (UF) OMWW fractions, in order to substantiate their use as natural cosmeceutical ingredient. Six chemically characterized UF-OMWW fractions, from Italian and Greek olive cultivar processing, were investigated for their antioxidant activities, measured by Trolox Equivalent Antioxidant Capacity (TEAC), LDL oxidation inhibition, and ROS-quenching ability in UVA-irradiated HEKa (Human Epidermal Keratinocytes adult) cultures. The photoprotective properties of UF-OMWW were assayed as a pro-oxidant-mediated pro-apoptotic effect on the UVA-damaged HEKa cells, which can be potentially involved in the carcinogenesis process. All the UF-OMWW fractions exerted an effective antioxidant activity in vitro and in cells when administered together with UV-radiation on HEKa. A pro-oxidative and pro-apoptotic effect on the UVA-damaged HEKa cells were observed, suggesting some protective actions of polyphenol fraction on keratinocyte cell cultures.
Using Bioelements Isotope Ratios and Fatty Acid Composition to Deduce Beef Origin and Zebu Feeding Regime in Cameroon
Authors: Perini Matteo , Nfor Mohamadou Bawe , Camin Federica , Pianezze Silvia , Piasentier Edi
Published: April 2021

The purpose of this study was to address the lack of knowledge regarding the stable isotopic composition of beef from zebu cattle reared in tropical Africa. Sixty beef carcasses belonging to the most common zebu breeds (Goudali, white Fulani, and red Mbororo) were selected and classified according to their subcutaneous fat color (white, cream or yellow). The stable isotope ratios of five bioelements—H, O, C, N, and S—in muscle fractions and the fatty acids composition were analyzed. Zebu meat from Cameroon shows peculiar δ13C values, related to the almost exclusive intake of grazed tropical grasses with photosynthetic cycle C4. It also shows δ2H and δ18O values higher than those reported in other areas of the world and correlated with the isotopic composition of animal drinking water. The white subcutaneous fat (“white type”) zebu showed higher δ2H and lower δ13C than the “yellow type”, that is correlated with a higher content of polyunsaturated fatty acid (PUFA) and a lower amount of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA). Multielement analysis seems to provide promising results for tracing the regional origin of Cameroon beef and some aspects of the livestock system, such as the nutritional status of the animals.
Prodigiosin of Serratia marcescens ZPG19 Alters the Gut Microbiota Composition of Kunming Mice
Authors: Li Xue , Tan Xinfeng , Chen Qingshuang , Zhu Xiaoling , Zhang Jing , Zhang Jie , Jia Baolei
Published: April 2021

Prodigiosin is a red pigment produced by Serratia marcescens with anticancer, antimalarial, and antibacterial effects. In this study, we extracted and identified a red pigment from a culture of S. marcescens strain ZPG19 and investigated its effect on the growth performance and intestinal microbiota of Kunming mice. High-performance liquid chromatography/mass spectrometry revealed that the pigment had a mass-to-charge ratio (m/z) of 324.2160, and thus it was identified as prodigiosin. To investigate the effect of prodigiosin on the intestinal microbiota, mice (n = 5) were administered 150 μg/kg/d prodigiosin (crude extract, 95% purity) via the drinking water for 18 days. Administration of prodigiosin did not cause toxicity in mice. High-throughput sequencing analysis revealed that prodigiosin altered the cecum microbiota abundance and diversity; the relative abundance of Desulfovibrio significantly decreased, whereas Lactobacillus reuteri significantly increased. This finding indicates that oral administration of prodigiosin has a beneficial effect on the intestinal microbiota of mice. As prodigiosin is non-toxic to mouse internal organs and improves the mouse intestinal microbiota, we suggest that it is a promising candidate drug to treat intestinal inflammation.
Native Structure-Based Peptides as Potential Protein–Protein Interaction Inhibitors of SARS-CoV-2 Spike Protein and Human ACE2 Receptor
Authors: Odolczyk Norbert , Marzec Ewa , Winiewska-Szajewska Maria , Poznański Jarosław , Zielenkiewicz Piotr
Published: April 2021

Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is a positive-strand RNA virus that causes severe respiratory syndrome in humans, which is now referred to as coronavirus disease 2019 (COVID-19). Since December 2019, the new pathogen has rapidly spread globally, with over 65 million cases reported to the beginning of December 2020, including over 1.5 million deaths. Unfortunately, currently, there is no specific and effective treatment for COVID-19. As SARS-CoV-2 relies on its spike proteins (S) to bind to a host cell-surface receptor angiotensin-converting enzyme-2(ACE2), and this interaction is proved to be responsible for entering a virus into host cells, it makes an ideal target for antiviral drug development. In this work, we design three very short peptides based on the ACE2 sequence/structure fragments, which may effectively bind to the receptor-binding domain (RBD) of S protein and may, in turn, disrupt the important virus-host protein–protein interactions, blocking early steps of SARS-CoV-2 infection. Two of our peptides bind to virus protein with affinity in nanomolar range, and as very short peptides have great potential for drug development.
Dragon Fruits as a Reservoir of Natural Polyphenolics with Chemopreventive Properties
Authors: Paśko Paweł , Galanty Agnieszka , Zagrodzki Paweł , Luksirikul Patraporn , Barasch Dinorah , Nemirovski Alina , Gorinstein Shela
Published: April 2021

Dragon fruits are a valued source of bioactive compounds with high potential to become a functional food. The aim of the study was to evaluate and compare the chemopreventive potential and chemical composition of fruits harvested in Thailand and Israel. The amount of different compounds in water and methanol extracts and antioxidant activity was investigated. Moreover, cytotoxic activity against cancer and normal cells of skin, prostate, and gastrointestinal origin was performed, accompanied by anti-inflammatory assay based on NO production in RAW 264.7 macrophage model. Additionally, the quenching properties of polyphenols from fruits were determined by the interaction of the main drug carrier in blood human serum (HSA). The chemometric analysis was used to reveal the relationships between the determined parameters. Dragon fruits harvested in Israel revealed higher antioxidant properties and total content of polyphenols and betacyanins when compared to those from Thailand. The examined fruits of both origins showed significant cytotoxic activity toward colon and prostate cancer cells, with no toxic effect on normal cells, but also no anti-inflammatory effect. Moreover, a high binding ability to HSA was observed for water extracts of dragon fruits. All these predestine dragon fruits are the candidates for the attractive and chemopreventive elements of daily diet.
A Straightforward Hypoxic Cell Culture Method Suitable for Standard Incubators
Authors: Matthiesen Svea , Jahnke Rico , Knittler Michael R.
Published: April 2021

We present a new and straightforward method by which standard cell culture plates can be sealed off from ambient air and be placed under controlled hypoxic cell culture conditions without costly or highly specialized materials. The method was established on a murine cell culture system using the dendritic cell line JAWS II but can be readily adapted to other cell cultures. The procedure was designed to be easy to implement in cell culture laboratories with standard incubators and requires only readily available materials, resources, and consumables, such as six-well plates, degassed culture medium, CoCl2, a vacuum sealer, etc., and no further complicated laboratory equipment. The simple hypoxic cell culture method presented here is technically reliable and experimentally safe. As it can be performed in any standard incubator, it is suitable for use at both low and higher biosafety levels.
Graphene Oxide/Reduced Graphene Oxide Enhanced Noniridescent Structural Colors Based on Silica Photonic Spray Paints with Improved Mechanical Robustness
Authors: Yu Jiali , Lee Cheng-Hao , Kan Chi-Wai
Published: April 2021

In contrast to traditional pigment colors, structural colors have developed a great potential in practical applications, thanks to their unique nonfading and color tunable properties; especially amorphous photonic structures with noniridescent structural colors have attracted considerable attention and their applications have expanded to more fields. Herein, graphene oxide (GO) and reduced graphene oxide (RGO) enhanced noniridescent structural colors with excellent mechanical robustness were established by a time-saving approach named spray coating, which allows for rapid fabrication of angular independent structural colors by spraying different photonic spray paints (PSPs) to ensure color multiplicity that was adjusted by the silica nanoparticles (SiO2 NPs) sizes onto the substrates. The incorporation of poly(methyl methacrylate-butyl acrylate) (PMB) improved the adhesion existing among SiO2 inter-nanoparticles and between SiO2 NPs and the substrates, taking advantages of the low glass transition temperature (Tg) of butyl acrylate derivative polymer and made PMB embedded PSPs coated patterns being imparted with good mechanical robustness and abrasive resistance. The peculiar light adsorption of GO and RGO across visible light spectrum facilitate higher color saturation. The improvement in color saturation of GO and RGO doped PSPs is expected to boost the promising applications in structurally colored paintings, inks and other color-related optical fields.
Highly Skin-Conformal Laser-Induced Graphene-Based Human Motion Monitoring Sensor
Authors: Jeong Sung-Yeob , Lee Jun-Uk , Hong Sung-Moo , Lee Chan-Woo , Hwang Sung-Hwan , Cho Su-Chan , Shin Bo-Sung
Published: April 2021

Bio-compatible strain sensors based on elastomeric conductive polymer composites play pivotal roles in human monitoring devices. However, fabricating highly sensitive and skin-like (flexible and stretchable) strain sensors with broad working range is still an enormous challenge. Herein, we report on a novel fabrication technology for building elastomeric conductive skin-like composite by mixing polymer solutions. Our e-skin substrates were fabricated according to the weight of polydimethylsiloxane (PDMS) and photosensitive polyimide (PSPI) solutions, which could control substrate color. An e-skin and 3-D flexible strain sensor was developed with the formation of laser induced graphene (LIG) on the skin-like substrates. For a one-step process, Laser direct writing (LDW) was employed to construct superior durable LIG/PDMS/PSPI composites with a closed-pore porous structure. Graphene sheets of LIG coated on the closed-porous structure constitute a deformable conductive path. The LIG integrated with the closed-porous structure intensifies the deformation of the conductive network when tensile strain is applied, which enhances the sensitivity. Our sensor can efficiently monitor not only energetic human motions but also subtle oscillation and physiological signals for intelligent sound sensing. The skin-like strain sensor showed a perfect combination of ultrawide sensing range (120% strain), large sensitivity (gauge factor of ~380), short response time (90 ms) and recovery time (140 ms), as well as superior stability. Our sensor has great potential for innovative applications in wearable health-monitoring devices, robot tactile systems, and human–machine interface systems.
Tumor-Treating Fields Therapy for Pediatric Brain Tumors
Authors: Makimoto Atsushi , Nishikawa Ryo , Terashima Keita , Kurihara Jun , Fujisaki Hiroyuki , Ihara Satoshi , Morikawa Yoshihiko , Yuza Yuki
Published: April 2021

Tumor-treating fields (TTFields) are alternating electric fields applied continuously to the brain by attaching two-pair arrays on the scalp. Although TTFields therapy has demonstrated efficacy against supratentorial glioblastoma (GBM) in adults, its safety and efficacy in children have not been confirmed. Despite differences in the genetic etiology of the adult and pediatric forms of GBM, both have certain clinical behaviors in common, allowing us to test TTFields therapy in pediatric GBM. Recently, several, pediatric case-series using TTFields therapy have been published, and a few, prospective, pediatric studies are ongoing. Because GBMs are extremely rare in pediatric patients, where they comprise a wide variety of genetic subtypes, these pediatric studies are feasibility studies targeting various types of malignant brain tumor. Although they are important for confirming the safety and feasibility of TTFields therapy in the pediatric population, confirming its efficacy against each type of pediatric brain tumor, including the GBM, is difficult. Our clinical research team, therefore, planned an investigator-initiated clinical trial targeting pediatric supratentorial GBMs (as in adults) with the aim of expanding regulatory approval of TTFields therapy for pediatric GBM treatment based on safety and exploratory efficacy data in combination with the accumulated evidence on adult GBMs.
World Health Organization High Priority Pathogens: Ophthalmic Disease Findings and Vision Health Perspectives
Authors: Kuthyar Sanjana , Anthony Casey L. , Fashina Tolulope , Yeh Steven , Shantha Jessica G.
Published: April 2021

Recent Ebola epidemics, the ongoing COVID-19 pandemic, and emerging infectious disease threats have highlighted the importance of global infectious diseases and responses to public health emergencies. Ophthalmologists are essential health care workers who provide urgent and emergent vision care services during outbreaks and address the ocular consequences of epidemic and pandemic infectious diseases. In 2017, the World Health Organization (WHO) identified high priority pathogens likely to cause a future epidemic with the goal of guiding research and development to improve diagnostic tests, vaccines, and medicines. These measures were necessary to better inform and respond to public health emergencies. Given the ocular complications associated with emerging infectious diseases, there is a need to recognize the ophthalmic sequelae for future vision health preparedness for potential future outbreaks. The WHO High Priority pathogens list provides a roadmap for ophthalmologists and subspecialty providers that will guide strategic areas of research for clinical care and preparedness for future pandemic threats. This review summarizes these key viral pathogens, summarizes major systemic disease findings, and delineates relevant ocular complications of the WHO High Priority pathogens list, including Crimean-Congo hemorrhagic fever, Filovirus diseases (Ebola virus disease and Marburg hemorrhagic fever), human Coronaviruses, Lassa Fever, Nipah virus infection, Zika, and Rift Valley fever.
Serological and Molecular Prevalence of Babesia caballi in Apparently Healthy Horses in Israel
Authors: Tirosh-Levy Sharon , Mazuz Monica , Savitsky Igor , Pinkas Dana , Gottlieb Yuval , Steinman Amir
Published: April 2021

Babesia caballi is a tick-borne hemoparasite of equines and one of the causative agents of equine piroplasmosis, which poses a great concern for the equine industry regarding animal welfare and international horse movement. The parasite is endemic in Israel; however, its seroprevalence in the area was never evaluated due to antigenic heterogenicity in the gene used in the commercially available kit. Blood samples were collected from 257 horses at 19 farms throughout the country and screened for the presence of anti-B. caballi antibodies via an indirect immunofluorescent antibody test (IFAT) and for the presence of parasite DNA by nested PCR. The seroprevalence of B. caballi was 69.6% and its molecular prevalence was 9.7%. The geographical area, horse’s sex, breed, housing, exposure to ticks, and specifically to Hyalomma marginatum, and co-infection with Theileria equi were found to be significantly associated with serologic exposure in univariable analysis, while the geographical area and horses’ sex remained significant in the multivariable analysis. The results of this study demonstrate a high level of exposure to B. caballi and identify important risk factors for infection. The difference between the serological and molecular prevalence, probably related to parasite clearance, is also highlighted.
Dishwashers as an Extreme Environment of Potentially Pathogenic Yeast Species
Authors: Kulesza Kamila , Biedunkiewicz Anna , Nowacka Karolina , Dynowska Maria , Urbaniak Monika , Stępień Łukasz
Published: April 2021

The study aimed to compare the yeast species diversity in the specific environment of dishwashers, taking into account the potential risk for users. Yeasts were isolated from ten dishwashers and from tap water supplied to the appliances. Samples were collected for mycological analyses at the beginning of each month, from February to May 2016. Four dishwasher sites (rubber seals, detergent dispensers, sprinklers, and water drains) were analyzed. The microfungi were identified by the standard procedures applied in mycological diagnostics. To confirm species identification, molecular analysis was performed based on the sequences of the D1/D2 region. The presence of microfungi was detected in 70% of the investigated appliances. Rubber seals, detergent dispensers, and water drains were the most frequently colonized elements. Thirty-five yeast strains were isolated in this study, of which twenty-seven were obtained from dishwashers and eight from tap water. The strains belonged to six genera and six species (Candida parapsilosis, Clavispora lusitaniae, Dipodascus capitatus, Exophiala dermatitidis, Meyerozyma guilliermondii, and Rhodotorula mucilaginosa). Most of the strains came from rubber seals. In this way, it was demonstrated that the dishwashers’ condition is sufficient as an ecological niche for microfungi.
Multilocus Sequence Analysis of Selected Housekeeping- and Pathogenicity-Related Genes in Venturia inaequalis
Authors: Michalecka Monika , Puławska Joanna
Published: April 2021

The relationship between housekeeping and pathogenicity-related genes and virulence or avirulence towards the primary Malus resistance genes (R) has not been previously studied for Venturia inaequalis fungus, the causal agent of apple scab. In this study, the sequences of two housekeeping genes encoding elongation factor alpha (EF-1α) and β-tubulin and two previously unstudied effector genes of V. inaequalis from mannosidase and glucosidase families of 100 strains collected from apple cultivars with Rvi6, Rvi1, and Rvi17 and without known scab resistance genes were submitted to the analyses. Based on the phylogenetic and diversity data, as well as recombination analyses of the sequenced regions, we assessed the phylogenetic relationships and genetic structure of the pathogen within the species and the evolutionary forces that are currently acting upon this microorganism. The topology of the obtained phylograms demonstrates the lack of a relationship between the phylogenetic position of the strain and the host cultivar and the geographical origin or race of the strain. The isolates from different hosts were differentiated but did not form diagnosable, distinct phylogenetic groups. These results suggest that the analyzed genes may be too conserved to reflect the adaptation of pathogens to apple genotypes with different R genes; thus, they do not adequately reflect race discrimination. In contrast, based on variation and gene flow estimation, genetic divergence was observed among strains virulent to apple trees containing Rvi6. The results of this study confirmed a lack of free recombination between strains and demonstrated that the analyzed regions are in linkage disequilibrium and contain non-random polymorphisms associated with the strain.
Preeclampsia Status Controls Interleukin-6 and Soluble IL-6 Receptor Release from Neutrophils and Endothelial Cells: Relevance to Increased Inflammatory Responses
Authors: Wang Yuping , Gu Yang , Alexander J. Steven , Lewis David F.
Published: April 2021

Increased neutrophil–endothelial binding and inflammatory responses are significant pathophysiological events in the maternal vascular system in preeclampsia, a hypertensive disorder in human pregnancy. Interleukin 6 (IL-6) and its soluble receptors (soluble IL-6R (sIL-6R) and soluble gp130 (sgp130)) are critical inflammatory mediators. During pregnancy, maternal IL-6 and sgp130 levels were increased, but sIL-6R levels were decreased, in women with preeclampsia compared to normotensive pregnant women. However, little is known about differences in IL-6, sIL-6R, and sgp130 production by neutrophils and endothelial cells between normal pregnancy and preeclampsia. To study this, we isolated neutrophils and cultured human umbilical vein endothelial cells (HUVECs) from normal and preeclamptic pregnancies. Production of IL-6, sIL-6R, and sgp130 was measured. The role of placental factor(s)-mediated neutrophil production of IL-6, sIL-6R, and sgp130 was also determined by pretreating neutrophils with placental conditioned medium generated from placental villous cultures. We found that IL-6 and sgp130 were mainly produced by endothelial cells, while sIL-6R was mainly produced by neutrophils. Endothelial cells from preeclampsia produced significantly more IL-6 and sgp130, and neutrophils from preeclampsia produced significantly less sIL-6R than normal pregnancy cells. Interestingly, production of IL-6, sIL-6R, and sgp130 were time-dependently increased when neutrophils and endothelial cells were co-cultured. We also found that neutrophils from normal pregnancies produced more IL-6, but less sIL-6R, after being primed by preeclamptic-placental conditioned medium. These results demonstrated that neutrophils and endothelial cells have different capacities in producing IL-6, sIL-6R, and sgp130 between normal pregnancy and preeclampsia. These results also provide evidence that the placenta plays a role in inducing neutrophil activation in preeclampsia.
Cardiopulmonary Exercise Testing Is an Accurate Tool for the Diagnosis of Pulmonary Arterial Hypertension in Scleroderma Related Diseases
Authors: Bellan Mattia , Giubertoni Ailia , Piccinino Cristina , Buffa Mariachiara , Cromi Debora , Sola Daniele , Pedrazzoli Roberta , Gagliardi Ileana , Calzaducca Elisa , Zecca Erika , Patrucco Filippo , Patti Giuseppe , Sainaghi Pier Paolo , Pirisi Mario
Published: April 2021

The early diagnosis of pulmonary arterial hypertension (PAH) is a major determinant of prognosis in patients affected by connective tissue diseases (CTDs) complicated by PAH. In the present paper we investigated the diagnostic accuracy of cardiopulmonary exercise testing (CPET) in this specific setting. We recorded clinical and laboratory data of 131 patients who underwent a CPET at a pulmonary hypertension clinic. Out of them, 112 (85.5%) had a diagnosis of CTDs; 8 (6.1%) received a diagnosis of CTDs-PAH and 11 (8.4%) were affected PH of different etiology. Among CPET parameters the following parameters showed the best diagnostic performance for PAH: peak volume of oxygen uptake (VO2; AUC: 0.845, CI95% 0.767–0.904), ratio between ventilation and volume of exhaled carbon dioxide (VE/VCO2 slope; AUC: 0.888, CI95%: 0.817–0.938) and end-tidal partial pressures (PetCO2; AUC: 0.792, CI95%: 0.709–0.861). These parameters were comparable among CTDs-PAH and PH of different etiology. The diagnostic performance was even improved by creating a composite score which included all the three parameters identified. In conclusion, CPET is a very promising tool for the stratification of risk of PAH among CTDs patients; the use of composite measures may improve diagnostic performance.
Pre-Emptive Priming of Human Skin Improves Cutaneous Scarring and Is Superior to Immediate and Delayed Topical Anti-Scarring Treatment Post-Wounding: A Double-Blind Randomised Placebo-Controlled Clinical Trial
Authors: Ud-Din Sara , Wilgus Traci A. , McGeorge Douglas D. , Bayat Ardeshir
Published: April 2021

The concept of pre-emptive priming of skin pre-surgery offers a novel approach in optimizing cutaneous scarring outcome. We previously showed an anti-scarring topical (epigallocatechin-3-gallate (EGCG)) is effective in improving skin scarring when applied post-surgery. The objective was to deliver an active compound at the optimal time in order to maximize its impact and improve cutaneous scarring. Therefore, pre-emptive application of anti-scarring topical pre-surgery compared with post-surgery can potentially be superior on scarring outcome. This double-blinded randomized placebo-controlled trial compares the effects of pre-emptive priming of skin with an anti-scarring topical pre-surgery versus post-surgery. Healthy volunteers (n = 40) were split into 4-groups; each undergoing different modes of application versus placebo: Group-1 = priming (7Days) pre-injury, Group-2 = priming (3D) pre-injury, Group-3 = immediate (0D) day-of-injury, Group-4 = delayed application (14D) post-injury. Excisional skin-biopsies in upper-arms were evaluated weekly with multiple quantitative devices over 8-weeks. Histological, immunohistochemical, mRNA sequencing and QRT-PCR studies were performed on tissue-biopsies. EGCG reduced mast cells at weeks-4 and 8 by gene and protein analyses (p < 0.01). Group 1 was superior to other groups (p < 0.01) in both clinical (blood flow) and laboratory parameters (elastin and immune marker expression). Additionally, there was down-regulation of angiogenic-markers by mRNA-sequencing and of CD31 and VEGF-A at weeks-4 and 8 (p < 0.01) by immunohistochemistry and at week-4 (p < 0.05) by QRT-PCR. EGCG increased antioxidant levels (HO-1) at week-4 (p < 0.01) plus elastin at week-8 (p < 0.01). In conclusion, pre-emptive priming of skin pre-injury has significant beneficial effects on surgically induced skin scarring shown by reducing mast cells, blood flow and angiogenesis plus increasing elastin content. This clinical trial was registered with ISRCTN (ISRCTN70155584).
A Questionnaire-Based Survey to Assess the Level of Knowledge and Awareness about Drug–Food Interactions among General Public in Western Saudi Arabia
Authors: Zaidi Syed Faisal , Mgarry Rayan , Alsanea Abdullah , Almutairi Sakar Khalid , Alsinnari Yaser , Alsobaei Saad , Ahmed Kanwal
Published: April 2021

Introduction: Various drug–food interactions exist that may hinder treatment and can sometimes be lethal. Our aim was to assess the level of public knowledge and awareness in Jeddah city, Western Saudi Arabia, about drug–food interactions, along with the effects of demographics on their knowledge. Methods: A survey questionnaire was administered in this cross-sectional study to participants spread across multiple locations in Jeddah, including in malls and public gatherings. Participants included both males and females. Sample size was calculated through Raosoft® software. Data analysis was executed using IBM Statistic SPSS and the level of statistical significance was set at p < 0.05. Results: A total of 410 people participated in the study and only 92.68% (380) of responses were enrolled in the study; 7.32% (30) were not enrolled due to the exclusion criteria. Surprisingly, only six out of eighteen questions regarding drug–food interactions in the administered questionnaire were correctly answered by 380 participants. Data indicated that the participants had a poor to intermediate level of both knowledge and awareness with respect to drug–food interactions. Furthermore, participants showed moderate to strong awareness of the effects of alcohol and tea generally, and their interaction with medication. Conclusion: Participants in our study showed inadequate knowledge of basic and fundamental information about drug–food interactions, which highlights the dire need to increase awareness.
Sulfophosphate Glass Doped with Er3+ and TiO2 Nanoparticles: Thermo-Optical Characterization by Photothermal Spectroscopy
Authors: Ebrahimpour Zeinab , Cabrera Humberto , Ahmadi Fahimeh , Asgari Asghar , Niemela Joseph
Published: April 2021

In this work, time-resolved thermal lens and beam deflection methods were applied to determine the thermo-optical properties of Er3+ doped sulfophosphate glass in which different concentrations of Titanium dioxide (TiO2) nanoparticles (NPs) were embedded. Thermal diffusivity (D), thermal conductivity (κ), and the temperature coefficient of the optical path length (ds/dT) were determined as a function of NPs concentrations. Moreover, the growth of TiO2 NPs inside the amorphous glass matrix was evidenced by Transmission Electron Microscopy (TEM) images as well as through optical effects such as refractive index change of the glass. The outcomes indicated relatively high values for D and κ as well as a low ds/dT as required for most optical components used for laser media. The addition of TiO2 NPs with concentration of dopants up to 0.6 mol% improved the optical properties of the glass samples but did not affect its thermal properties. The results indicate that the enhanced optical and thermal performance of the proposed co-doped glass fits the quality standards for materials used in photonic devices.
Ballistic Performance of Guaruman Fiber Composites in Multilayered Armor System and as Single Target
Authors: Reis Raphael , Nunes Larissa , da Luz Fernanda , Candido Verônica , da Silva Alisson , Monteiro Sergio
Published: April 2021

Multilayered armor systems (MAS) with a front ceramic layer backed by a relatively unknown Amazonian guaruman fiber-reinforced (Ischnosiphon koem) epoxy composites, as second layer, were for the first time ballistic tested against the threat of 7.62 mm rifle ammunition. The amount of 30 vol% guaruman fibers was investigated in three distinct configurations: (i) continuous aligned, (ii) 0–90° cross-laid, and (iii) short-cut randomly dispersed. Additionally, single-target ballistic tests were also carried out in the best MAS-performed composite with cross-laid guaruman fibers against .22 caliber ammunition. The results disclosed that all composites as MAS second layer attended the US NIJ standard with corresponding penetration depth of (i) 32.9, (ii) 27.5, and (iii) 29.6 mm smaller than the lethal limit of 44 mm in a clay witness simulating a personal body. However, the continuous aligned guaruman fiber composite lost structural integrity by delamination after the 7.62 projectile impact. By contrast, the composite with cross-laid guaruman fibers kept its integrity for subsequent shootings as recommended by the standard. The single-target tests indicated a relatively higher limit velocity for .22 caliber projectile perforation, 255 m/s, and absorbed energy of 106 J for the cross-laid guaruman fibers, which are superior to corresponding results for other less known natural fiber epoxy composites.
Use of Solid-State NMR Spectroscopy for the Characterization of Molecular Structure and Dynamics in Solid Polymer and Hybrid Electrolytes
Authors: Foran Gabrielle , Verdier Nina , Lepage David , Malveau Cédric , Dupré Nicolas , Dollé Mickaël
Published: April 2021

Solid-state NMR spectroscopy is an established experimental technique which is used for the characterization of structural and dynamic properties of materials in their native state. Many types of solid-state NMR experiments have been used to characterize both lithium-based and sodium-based solid polymer and polymer–ceramic hybrid electrolyte materials. This review describes several solid-state NMR experiments that are commonly employed in the analysis of these systems: pulse field gradient NMR, electrophoretic NMR, variable temperature T1 relaxation, T2 relaxation and linewidth analysis, exchange spectroscopy, cross polarization, Rotational Echo Double Resonance, and isotope enrichment. In this review, each technique is introduced with a short description of the pulse sequence, and examples of experiments that have been performed in real solid-state polymer and/or hybrid electrolyte systems are provided. The results and conclusions of these experiments are discussed to inform readers of the strengths and weaknesses of each technique when applied to polymer and hybrid electrolyte systems. It is anticipated that this review may be used to aid in the selection of solid-state NMR experiments for the analysis of these systems.
Flow and Heat Transfer Property of Oldroyd-B-Fluid-Based Nanofluids Containing Cylindrical Particles in a Pipe
Authors: Lin Wenqian , Zhang Peijie , Lin Jianzhong
Published: April 2021

Flow and heat transfer property of Oldroyd-B-fluid-based nanofluids containing cylindrical particles are studied in a pipe with circular cross-section in the range of Reynolds number (Re) from 100 to 2000, Weissenberg number (We) from 0.1 to 2, particle aspect ratio (β) from 2 to 16 and particle volume concentration (Φ) from 0.1% to 2.5%. The motion equation of Oldroyd-B fluid with particles, the equation for probability density function of particle orientation and convection-diffusion equation for particles are solved numerically. The numerical method used in the simulation is validated by comparing with the available results. The effects of Re, We, β and Φ on the friction factor (f), Nusselt number (Nu) and ratio of energy performance evaluation criterion (PECt/PECf) for Oldroyd-B-fluid-based nanofluids to that for Oldroyd-B fluids are discussed. The results showed that the values of f and Nu of Oldroyd-B-fluid-based nanofluids are larger than that of water-based nanofluids and that of pure Oldroyd-B fluids. The values of f increase with increasing Re, We and Φ, but with decreasing β. The values of Nu and PECt/PECf are enhanced with increasing Re, We, β and Φ. The increase of f is larger than that of Nu at lower Re, but is less than that of Nu at higher Re. It is more effective to use Oldroyd-B-fluid-based nanofluids with cylindrical nanoparticles to improve the heat transfer at the conditions of higher Re, We, β and Φ. Finally, the correlation formula of PECt/PECf as a function of Re, We, β and Φ is derived.
Batch and Semi-Continuous Anaerobic Digestion of Industrial Solid Citrus Waste for the Production of Bioenergy
Authors: Rosas-Mendoza Erik Samuel , Alvarado-Vallejo Andrea , Vallejo-Cantú Norma Alejandra , Snell-Castro Raúl , Martínez-Hernández Sergio , Alvarado-Lassman Alejandro
Published: April 2021

The aim of this paper is to describe a study of the anaerobic digestion of industrial citrus solid waste (ISCW) in both batch and semi-continuous modes for the production of bioenergy without the elimination of D-limonene. The study was conducted at the pilot plant level in an anaerobic reactor with a working volume of 220 L under mesophilic conditions of 35 ± 2 °C. Cattle manure (CM) was used as the inoculum. Three batches were studied. The first batch had a CM/ISCW ratio of 90/10, and Batches 2 and 3 had CM/ISCW ratios of 80/20 and 70/30, respectively. In the semi-continuous mode an OLR of approximately 8 g total chemical oxygen demand (COD)/Ld (4.43 gVS/Ld) was used. The results showed that 49%, 44%, and 60% of volatile solids were removed in the batch mode, and 35% was removed in the semi-continuous mode. In the batch mode, 0.322, 0.382, and 0.316 LCH4 were obtained at STP/gVSremoved. A total of 24.4 L/d (34% methane) was measured in the semi-continuous mode. Bioenergy potentials of 3.97, 5.66, and 8.79 kWh were obtained for the respective batches, and 0.09 kWh was calculated in the semi-continuous mode. The citrus industry could produce 37 GWh per season. A ton of processed oranges has a bioenergy potential of 162 kWh, which is equivalent to 49 kWh of available electricity ($3.90).
Experimental Study on the Flexural Behavior of Concrete-Filled Steel Box Slabs
Authors: Lin Yan , Zhong Chongting , Song Jiankai , Qu Shuang
Published: April 2021

Different to conventional reinforced concrete or steel material, a new type of concrete-filled steel box slab has been proposed by effectively integrating the combined benefits of both steel and concrete. By filling concrete, this type of slab could avoid the local bucking of steel plate and then could provide a high moment capacity. Therefore, this study aims to investigate the flexural behavior of concrete-filled steel box slabs through testing seven specimens and characterize their failure characteristics, stress distribution and ultimate moment capacity. A comprehensive parametric study focuses on the influence of the steel plate thickness, the stud and stiffening plate on the slab flexural behavior. The results show that concrete-filled steel box slabs had a high moment capacity and good ductility. The thickness of the steel plate had a significant influence on the moment capacity of the specimen. The stud led to the reduction of the moment capacity of the slab. The stiffening plate effectively improved the flexural behavior of the specimen. Moreover, a calculation method was formulated to describe the moment capacity of the slab and compared with experimental results. The results show that the proposed method for the moment capacity of the slab was conservative and reasonable.
Measuring and Modeling the Solubility of Hydrogen Sulfide in rFeCl3/[bmim]Cl
Authors: Cheng Huanong , Li Na , Zhang Rui , Wang Ning , Yang Yuanyuan , Teng Yun , Jia Wenting , Zheng Shiqing
Published: April 2021

The solubility of hydrogen sulfide in different mole ratios of ferric chloride and 1-butyl-3-methylimidazolium chloride ionic liquid (rFeCl3/[bmim]Cl, r = 0.6, 0.8, 1.0, 1.2, 1.4) at temperatures of 303.15 to 348.15 K and pressures of 100 to 1000 kPa was determined. The total solubility increased with the increase of pressure and the decrease of temperature. The solubility data were fitted using the reaction equilibrium thermodynamic model (RETM). The mean relative error between the predicted value and the measured value was less than 4%. Henry’s coefficient and the equilibrium constant of chemical reaction at each temperature were calculated. Henry’s coefficient first decreased and then increased with the increase of mole ratio, and increased with the increase of temperature. The equilibrium constant of the chemical reaction followed the same law as Henry’s coefficient. The chemical solubility was related to both Henry’s coefficient and the chemical equilibrium constant. H2S had the highest chemical solubility in FeCl3/[bmim]Cl at a mole ratio of 0.6 and a temperature of 333.15 K. The chemical solubility increased with the increase of pressure.
Model-Based Evaluation of a Data-Driven Control Strategy: Application to Ibuprofen Crystallization
Authors: Montes Frederico C. C. , Öner Merve , Gernaey Krist V. , Sin Gürkan
Published: April 2021

This work presents a methodology that relies on the application of the radial basis functions network (RBF)-based feedback control algorithms to a pharmaceutical crystallization process. Within the scope of the model-based evaluation of the proposed strategy, firstly strategies for the data treatment, data structure and the training methods reflecting the possible scenarios in the industry (Moving Window, Growing Window and Golden Batch strategies) were introduced. This was followed by the incorporation of such RBF strategies within a soft sensor application and a nonlinear predictive data-driven control application. The performance of the RBF control strategies was tested for the undisturbed cases as well as in the presence of disturbances in the process. The promising results from both RBF soft sensor control and the RBF predictive control demonstrated great potential of these techniques for the control of the crystallization process. In particular, both Moving Window and Golden Batch strategies performed the best results for an RBF soft sensor, and the Growing Window outperformed the remaining methodologies for predictive control.