bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of non – allelic interactions of maize endosperm structure genes on the oil fatty acid composition
Authors: Tymchuk D. S. , Muzhylko V. V. , Tymchuk S. M.
Published: July 2020

Aim. Determination of effects of the non-allelic interactions between the different genes of maize endosperm structure and identification the carriers of combinations of mutant genes with the best oil fatty acid compositions. Methods. The representative complex of maize inbreds – carriers of mutant genes o2, sh1, sh2, su1, su2, ae and wx and their paired combination was used as the material for research. Oil fatty acid composition was analysed by the modified gas – chromatographic Peysker method. Statistical analysis of the results was performed by the method of variance analysis. Results. The carriers of endospermic mutation su1 were notable as having the most high content of oleate in oil and the carriers of mutation sh2 – as having the most high palmitate content. The non–allelic interactions of endosperm structure genes for the oil fatty acid composition were realized by three types. The first of them took place immediate level of components of the oil fatty acid composition between the mutants who were partners of the combination. The second type was characterized by the presence of epistatic deviation of some components of the fatty acid composition in the direction of one the monogenic partners of combination. In the third type it has been observed the synergistic increase or reduction of the components of the fatty acid composition compared with both partners of combination. Conclusions. Use of non–allelic interactions between the mutant genes of maize endosperm structure can be observed as an effective method of the extension of useful genetic diversity while improving the oil quality. The most significant beneficial effects of non – allelic interactions of the synergistic type were provided by the combinations involving the mutant genes su1 and sh2.Keywords: maize, endospermic mutants, non-allelic interactions, oil fatty acid composition.
Directions of buckwheat varieties creation for cultivation in summer crops
Authors: Trotsenko V. I. , Klitsenko A. V.
Published: July 2020

Aim. The culture of buckwheat is traditional for our country. A number of economic and social factors of the last decades caused a significant decrease of its production that led to decrease an internal consumption and export potential losses. One of the possible ways to solve this problem is expanding the range of varietal spectrum of buckwheat culture at the expense of genotypes oriented on summer (stubble) sowing with the development of appropriate cultivation technologies. The prerequisites for this are the steady tendencies to the warming climate in the traditional areas of buck-wheat cultivation. Methods. The data of buckwheat samples collection environmental testing in the northern east forest-steppe (NEFS) of Ukraine conditions has been analyzed by statistical processing analyses. Results. The general plan of works envisaged the research of the buckwheat samples collection under the analyzing background conditions, the selection of group and individual characteristics of the different genotypes by response to photoperiod; the previous evaluation of the selection directions and the effectiveness of it. Conclusions. The analysis of the growing season conditions dynamics, the interphase periods length and plants productivity of the spring and summer sowing indicates the advisability of the separate plant breeding program for creating the varietal technology for summer crops cultivation for NEFS area.

Keywords: buckwheat, variety, photoperiod, growing season length, stubble sowing.
Adaptive potential of source material for bread spring wheat breeding
Authors: Khomenko S. O. , Fedorenko I. V. , Fedorenko M. V. , Blyzniuk R. M. , Kuzmenko Ye. A.
Published: July 2020

Aim. The research was aimed to allocate bread spring wheat lines of competitive strain testing with high adaptive potential to engage in research programs as a source material. Methods. Statistical. Results. Meteorological conditions during the period studied were contrast, which allowed to evaluate and highlight lines of bread spring wheat for adaptability. Based on the analysis of parameters of yield and its variability under the influence of environmental factors during the 2013–2015 lines with high adaptive capacity: Lutestsens 10-29, Lutestsens 11-26, Lutestsens 10-36, Erytrospermum 13-39, Lutestsens 08-26 were identified. Lines with high adaptive capacity Lutestsens 10-36, Erytrospermum 13-39, Lutestsens 08-26 was transferred to the State variety testing as Oksamyt Mironivs’kyi, MIW Oleksandra and Bozhena. Conclusions. Genotypic variability of performance elements of bread spring wheat lines of competitive strain testing was determined. The lines with high adaptive capacity were identified.

Keywords: bread spring wheat, source material, productivity, adaptability.
Multiflorous forms of soybean with long inflorescence are important source of breeding on increase of the seed productivity
Authors: Shcherbyna O. Z. , Mykhailov V. G. , Tymoshenko O. O.
Published: July 2020

Aim. The detailed research of plant-breeding value of the synthesized multiflorous forms with long inflorescence. Methods. The field and laboratory experiments for study of quantitative signs of varieties and hybrids with the statistical processing of data. Results. Synthesized multiflorous forms of soybean were characterized by greater duration of period of vegetation (135–150 days) and high of plants (100–160 cm). On the signs of inflorescence multiflorous forms were distinguished from existent varieties by the amount of flowers in inflorescence – from 9.2 to 32.8 (at plant-breeding varieties – 2.6–5.0), long inflorescence – to 18.6 and more cm (at plant-breeding varieties are 0.3 cm to 1.8 cm). These forms were characterized by the greater amount of pods and seed from a plant, by mass of seed from a plant, and also by the greater amount of knots, amount of branches of first-order than plant-breeding varieties and numbers. The hybrids of first generation by the productivity considerably exceeded both paternal forms. Mass of seed from a plant changed from a 32.00 g to 60.61 g. On duration of period of vegetation the hybrids of first generation were inter-mediate between paternal forms. Conclusions. Multiflorous forms of soybean with long inflorescens are important source of breeding work on increase of seed productivity.

Keywords: soybean, multiflorous inflorescence, long inflorescence, varieties, hybrids.
Effects of various salt concentrations on germination of seeds and morphometric parameters of diploid wheat seedlings
Authors: Gasimova F. I. , Khanishova M. A. , Taghiyeva K. R. , Azizov I. V.
Published: July 2020

Aim. The aim of the research was the assessment of salt-tolerance of diploid wheat genotypes Triticum monococcum and Triticum dicoccum at the grain germination stage. Methods. To determine morphometric parameters of salt tolerance of wheat varieties, seeds were germinated in a NaCl solution and in distilled water using the roll method. Screening for salt tolerance of wheat genotypes was conducted at 100, 150 and 200 mM concentrations of NaCl. Seeds were kept on wet filter paper, at 20–22 ℃ temperature, in the dark for 3 days and then germinated under 12h/12h (light/dark) photoperiod for 7 days. Results. Germination energy for Tr. monococcum was 85–50 % and for Tr. dicoccum 85–70 % under salinity. Growth dynamics of the root system of diploid wheat genotypes during 10 days showed that the growth rate declined with increasing salt concentrations, and this decline was different in the genotypes. Conclusions. The results suggest that according to the characteristics of salt tolerance of diploid wheat varieties at the germination stage Tr. monococcum is a sensitive and Tr. dicoccum, which manifested relatively high parameters, is a tolerant variety.

Keywords: wheat genotypes, Triticum monococcum, Triticum dicoccum, salt stress, germination.
Effects of SNPs CAPN316 and CAST282 on quantitative characteristics of offspring produced by dairy and beef bulls
Authors: Ruban S. Yu. , Lysenko N. G. , Mitioglo L. V. , Goraichuk I. V. , Kolesnyk A. I. , Fedota A. M.
Published: July 2020

Aim. The aim of the study was to analyze effects of SNPs CAPN316 in CAST282 in calpain and calpastatin genes on offspring productive traits produced by dairy and beef bulls. Methods. Data on offspring productivity included milk performance traits (for dairy bulls), birth weight and average daily gain (for beef bulls). Molecular genetic analysis was performed by PCR-RFLP. χ²-, t-tests and Pearson correlation coefficient were used for statistical analysis at significance levels of 0.05. Results. In progeny of dairy bulls number of alleles C in both SNPs demonstrated negative correlation with milk yield (r=-0.577), fat yield (r=-0.794) and protein yield (r =-0.798). G allele of CAPN316 (“wild type”) was associated with increased number of beef bulls progeny. Body weight and average daily gain was better in GG-bulls offspring. Conclusions. C alleles of SNPs CAPN316 in CAST282 being associated with meat quality traits were found to be ineffective in selection for milk production traits.

Keywords: calpain and calpastatin genes, SNP, CAPN316, CAST282, bull’s genotype, evaluation on progeny, offspring productivity.
Effect of Rhizobium melilotti Tn5 mutant with changed lipopolysaccharides on manifestation of alfalfa mycoplasmosis
Authors: Korobkova E. S. , Zatovskaya T. V. , Patyka V. Ph.
Published: July 2020

Aim. Improving of plant safety functions by strengthening their physiological state and assimilation of nutrients is of particular importance as environmentally sound way of reducing the harmfulness of plant mycoplasmosis. The aim was to establish the effect of rhizobia including of mutant with modified polysaccharide characteristics which enter into symbiosis with leguminous plants to the manifestation of experimental mycoplasmosis of plants in the laboratory conditions. Methods. Tn5-transposon mutagenesis, polyacrylamide gel electrophoresis, microbiological methods, microvegetation experiment, biometric measurements. Results. From typical highly effective strain Rhizobium meliloti 425a it was obtained mutant Tb29 which differed from the parent strain on the synthesis of lipopolysaccharide. Medicago sativa plants inoculated by mutant were green and did not differ from plants with the parent strain. Acholeplasma infection of alfalfa without rhizobia treatment led to a certain stimulation of plants, that was manifested in some increasing of plant mass and size, but simultaneously causing symptoms of yellowing and leaf shape change. Conclusions. Formation of symbiotic relationships between M. sativa and R. meliloti 425a and Tb29 led to improvement of plant state and reduces the negative effect of plant pathogenic mollicute Acholeplasma laidlawii var. granulum118.

Keywords: mollicutes, plant pathogenic acholeplasma, rhizobia, mutant, lipopolysaccharides.
Characteristic of ITS fragment of rDNA of Rhodotorula mucilaginosa isolated from gastrointestinal tract of centenarian
Authors: Polishchuk L. V. , Tkachenko K. S. , Fomyna M. A.
Published: July 2020

Aim. The aim of the work was to analyse the primary structure of the ITS region of the ribosomal operon of the red yeast isolate Rhodotorula mucilaginosa S1 from the gastrointestinal tract of healthy centenarian compared to the available homological basidimycetous nucleotide sequences. Methods. In silico comparison of the ITS nucleotide sequences using NCBI server resources (BLAST programs and "Nucleotide" database) with the reference ITS-fragment of red yeast isolate was performed. Results. Almost 90 % of highly homologous to the reference ITS-fragment out of 800 selected sequences represented genera Rhodotorula and Rhodosporidium with the top hits for R. mucilaginosa strains PMM08-1716L, 3942L and 3684L. The most frequent nucleobases mismatches of ITS-fragments of GenBank’s R. mucilaginosa strains compared to the reference one were observed in the positions 541 (T/C) for 85 % of strains, and 129 (T/C) and 158 (G/A) for 26 % of strains. Conclusions. The primary structure of studied ITS region showed 100 % homology to R. mucilaginosa strains also isolated from humans and animals, as well as the most frequently mismatches for 129th, 158th and 541st nucleotides located in the spacers regions of the ribosomal operon.

Keywords: ITS, rDNA, nucleotide sequence, red yeast, human GIT.
Phylogenetic analysis of lysine, threonine and butanol strain-producers
Authors: Tigunova O. O. , Andriiash G. S. , Beiko N. Ye. , Shulha S. M.
Published: July 2020

Aim. Identify and confirm the taxonomic position of the obtained mutant strains Brevibacterium sp. IMB B-7447, Brevibacterium flavum IMB B-7446 and Clostridium acetobutylicum IMB B-7407 (IFBG C6H). Methods. A fragment of genomic DNA from agarose gel using «Macherey-Nagel NucleoSpin Extract» was isolated according to the instructions of the producer and sequenced. Comparative analysis using the program «BLAST-online» was done. Phylogenetic dendrograms using methods Neighbour joining and Maximum likelihood were created. Results. Dendrograms of phylogenetic relationships of studied strains and related strains of databases «GenBank» were constructed. Conclusions. Found that homology of Brevibacterium sp. 90 and Brevibacterium sp. IMB B-7447 is 98 %, and stains B. flavum TH7 and B. flavum IMB B-7446 classified to species C. glutamicum. Strain C. acetobutylicum IMB B-7407 (IFBG C6H) been reclassified to C. pasteurianum IFBG C6H. Keywords: genes 16S rRNA, dendrogram, stains-produsers.
Cardiac deletion of α-E-catenin leads to redused expression of the main component of desmosomes – γ-catenin
Authors: Balatskyi V. V. , Matsevych L. L. , Piven O. O.
Published: July 2020

Aim. In our present work, we have addressed to the γ-catenin, known main component of desmosomes, expression in hearts with heterozygous and homozygous knockout of α-E-catenin gene. Methods. Alpha-E-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. We analyze expression of γ-catenin with real time qPCR and Western blot. Results. Cardiac α-E-catenin deletion leads to downregulation of γ-catenin mRNA and protein levels only in homozygous mice, while we not observed any perturbation of γ-catenin expression in heterozygous mice. Conclusions. We have shown that homozygous knockout of α-E-catenin gene in embryonic heart occur reduction of the main component of desmosomes – γ-catenin mRNA and protein level of expression, which can lead to disruption of the desmosomes structure in adult myocardium.

Keywords: α-E-catenin, heart failure, γ-catenin.
The frequency of terminal deletions sY160 among men with microdeletions of AZFc region Y-chromosome
Authors: Haiboniuk I. Ye. , Tyrkus M. Ya. , Makukh H. V.
Published: July 2020

Aim. Determine the frequency of absence marker terminal deletions sY160 among men with microdeletions of AZFc region Y-chromosome. Methods. DNA from probands blood samples was isolated using a modified salting out method. Microdeletions of Y chromosome AZF region were analyzed using two multiplex PCR. The molecular-genetic study of terminal deletions (absence of sY160) was carried out using allele-specific PCR and analysed by electrophoresis in a 2 % agarose gel. Results. Among infertile men (1500 individuals), Y chromosome microdeletions were detected in 7% males: microdeletions of AZFa subregion in 1 %, AZFb subregion – 3 %, AZF(b+c) subregions – 15 %, AZFc subregion – 67 %. The presence of heterochromatin marker sY160 was confirmed in 39 cases (84.8 %) and absence of sY160 in 7 men (15.2 %). Absence of sY160 was detected in 2 men with AZFc microdeletion and in 5 men with AZFb+AZFc microdeletions. It is important to point out that terminal AZFc deletion was confirmed in 83.3 % of cases of AZFb+c microdeletions and only in 5.1 % of isolated AZFc microdeletions. Conclusions. Thus, among 15.2 % man with different AZF microdeletions of Y-chromosome the heterochromatin marker of terminal deletion sY160 was absents. The implementation of testing of marker of terminal deletion – sY160 may help to determine if the deletion corresponds to the b2/b4 pattern and to avoid biopsy in man which most likely not benefit from the surgical procedure.

Keywords: male infertility, spermatogenesis, Y chromosome, AZF region, terminal deletions.
Distribution of allelic variants of genes inhibitors and activators ectopic calcification in patients with acute coronary syndrome
Authors: Harbuzova V. Yu. , Obukhova O. A. , Rozumenko I. O. , Dubovyk Ye. I. , Oleshko T. M. , Harbuzova Ye. A. , Shvachko D. V. , Ataman O. V.
Published: July 2020

Aim. The study of the distribution of polymorphic variants K121Q (gene ENPP1), T134967G (gene ANKH) and A69314G (gene TNAP) is in patients with acute coronary syndrome. Methods. Venous blood of 118 patients with ACS and 110 persons of control group were genotyped for the polymorphism by PCR and restriction fragment length polymorphism method. Results. There is an association between acute coronary syndrome and polymorphic variants of genes TNAP (A69314G) and ANKH (T134967G). ACS risk in carriers of the minor allele of A69314G polymorphism to 2.2 times (P = 0.013; OR = 2.244), and for T134967G polymorphism to 1.9 times (P = 0.024; OR = 1.857) higher than homozygotes for the major allele. Conclusions. Allelic polymorphism gene activators and inhibitors of ectopic calcification is an important factor in hereditary susceptibility to sclerotic lesions of arteries and their complications.

Keywords: gene polymorphism, calcification, acute coronary syndrome.
Relative telomere length of the residents of Lviv oblast
Authors: Huleiuk N. L. , Tyrka M.
Published: July 2020

Aim. Telomeres are short, tandem repeats of DNA that cap linear chromosome ends by binding members of the shelterin protein complex to form protective telomere loops. An insufficient number of telomere repeats leads to chromosome uncapping, cell senescence, and death. Aim of this thesis is the analysis of relative telomere length (RTL) in whole blood among residents of Lviv region. Methods. The RTL in the whole blood cells was measured in 86 residents of various age (47 men and 39 women aged 18–72) using quantitative real-time PCR (Cawthon’s method). It is based on the simultaneous amplification of telomeric repeats (T) and a single copy gene (S). Results. There is a tendency to decrease RTL with age. The low correlation between RTL and age can be linked to various factors, including the heterogeneity of telomere length at birth, chronic socioeconomic stress, genetic determinism, sensitivity to exogenous pressures. Women and men did not differ significantly in the rate of RTL shortening. Conclusions. The results indicate the absence of reliable differences relative telomere length in individuals of different sexes. The low correlation between RTL and age can be linked to various factors, including insufficient sample size of people aged over 50 years. So in the future we plan to continue these studies in older people.

Keywords: relative telomere length, RT-PCR, age, gender.
Results of mutation status gene HER-2/NEU in patients with breast cancer in Ukraine
Authors: Klimuk B. T. , Dugan O. M. , Stefanovych A. V. , Klymenko S. V.
Published: July 2020

Aim. Detection of changes in epidermal growth factor receptor 2 (HER-2/Neu) in tumor cells is critical to patient prognosis, prediction of response to standard chemotherapy, and perspectives for the treatment of anti-HER-2 therapy. Methods. The research study was conducted according to the recommendations of ASCO/CAP 2013 to diagnose cases of determining gene expression HER-2/Neu by FISH. To identify the exact status of the gene HER-2/Neu TP53 marker was selected. Results. A statistically significant difference (χ2 = 3.36, p>0.05) was found among the questionable cases of mutated HER-2 / neu in the patient groups which was surveyed according to 2007 and 2013 years recommendations. Conclusions. In comparison with 2007 ASCO/CAP 2007 (from 1.4 % to 4.2 %) 2013 ASCO/CAP showed more cases which was classified as doubtful during the test with two-color breakdown HER-2/CEP17.

Keywords: HER-2/Neu, mutation status, FISH, centromere area, TP53.
XRCC1 codon 399 polymorphism (RS25487) in the Ukrainian population
Authors: Mishchuk Ya. M. , Kharkivska Ye. V. , Serha S. V. , Shkliar S. Ye. , Katrii V. B. , Koliada O. K. , Stakhovskyi O. E. , Stakhovskyi E. O. , Vitruk Yu. V. , Kononenko A. A. , Demydov S. V. , Ostapchenko L. I. , Kozeretska I. A.
Published: July 2020

Aim. To estimate the frequency of XRCC1 codon 399 polymorphic variants in bladder cancer patients and in a control group and define association of this polymorphism with a bladder cancer in Ukrainian patients. Methods. We determined the allele frequencies for 111 patients and 92 controls. Genotyping was performed by PCR-RELP method. Results. The distribution of genotypes in control group was: Arg/Arg – 48 % (n=44), Arg/Gln – 41.3 % (n=38), Gln/Gln – 10.7 % (n=10), whereas in group of patients with a bladder cancer the following distribution was observed: Arg/Arg – 56.8 % (n=63), Arg/Gln – 27.9 % (n=31), Gln/Gln – 15.3 % (n=17). Genotype distribution in control group was within Hardy-Weinberg equilibrium (χ2=59.7, p<0.0001), whereas in patient group it was not (χ2=0.172, p=0.678). No significant association was observed between the XRCC1 Arg399Gln polymorphism and bladder cancer risk. Conclusions. It is indicated that XRCC1 codon 399 polymorphism may not contribute to bladder cancer susceptibility in the Ukrainian population. Keywords: bladder cancer, polymorphism, XRCC1 gene, the cancer risk.
Allocation features and frequency of KIR-genes in women with regular early reproductive losses
Authors: Terpyliak O. I. , Zastavna D. V. , Helner N. V. , Osidach S. V.
Published: July 2020

Aim. KIR (killer cell immunoglobulin-like receptors) are the transmembranous glycoprotein receptors, role of which is to activate or inhibit the functional activity of cells, the surfaces of which they are located on. Genetically determined balance between KIR-activating and KIR-inhibiting receptors can contribute to some diseases predisposition. In this work we have studied the spectrum and frequency of KIR-genes among women with regular early idiopathic reproductive failures. Methods. Gene typing was conducted by PCR-SSP method. Results. Typing of KIR-genes was conducted in 31 DNA samples, extracted from peripheral blood cells from women without reproductive losses and in 38 DNA samples, extracted from peripheral blood cells from women with early reproductive losses of idiopathic nature. We found, that in women with reproductive failures genotypes of KIR-genes were characterized by decreased amount of activating genes. The presence of 2DL3 and 2DL5 genes increases the risk of early reproductive failures in 2 and 3 times, respectively. Conclusions. The shift of repertoire of KIR-genes into the domination of inhibitory genes and the spectrum features of KIR-genes can increase the risk of early reproductive failures.

Keywords: KIR-genes, recurrent pregnancy loss, PCR-SSP.
The contribution of chromosomal abnormalities in the formation of sporadic and recurrent early reproductive losses
Authors: Tkach I. R. , Huleiuk N. L. , Zastavna D. V. , Bezkorovaina G. M. , Fedyshyn T. V.
Published: July 2020

Aim. The aim of the present study was to investigate the contribution of numerical chromosomal imbalances in products of conception from sporadic pregnancy loss (SPL) and recurrent pregnancy loss (RPL). Methods. Banding cytogenetic and interphase mFISH with the probe panel for chromosomes 13, 14, 15, 16, 17, 18, 21, 22, X and Y. Results. Cytogenetic studies of 419 spontaneously aborted fetuses were performed. The results were stratified in 3 groups according to anamnesis: Group I and II – SPL, Group III – RPL. The contribution of chromosomal aberrations was higher in the genesis of SPL (39.9 %) compared to RPL (27.1 %). Among most often diagnosed chromosomal change was triploidy – 27.19 % in SPL vs 27.78 % in RPL, monosomy X – 21.93 % vs 22.22 % and trisomy 16–18.42 % vs 19.44 %. Conclusions.Consequently, detection of chromosomal aneuploidies in samples from products of conception plays a key role to find out about reasons of reproductive failure in humans.

Keywords: sporadic pregnancy loss, recurrent pregnancy loss, banding cytogenetic, interphase multicolor fluorescence in situ hybridization (mFISH), chromosome abnormalities.
Verification allelic polymorphism's of genes MTHFR and MTR in patients with psoriasis
Authors: Fedota A. M. , Roshcheniuk L. V. , Tyzhnenko T. V. , Admakina A. V. , Horaichuk I. V. , Vorontsov V. M.
Published: July 2020

Aim. Analysis of single nucleotide polymorphisms C677T, A1298C and A2756G of MTHFR and MTR one-carbon metabolism genes in patients with various forms of psoriasis in Ukrainian population. Methods. A molecular genetic analysis of 77 patients with vulgaris and arthropathic psoriasis by PCR-RFLP was carried out. Results. In patients with vulgaris and arthropathic psoriasis analysis of the distribution of frequencies of genotypes showed a statistically significant difference between them for C677T polymorphic variants. In patients with psoriasis analysis of genotype distribution of series in the two genes as a whole, showed a statistically significant difference between the theoretically expected and actual frequencies for single nucleotide polymorphisms A1298T and A2756G of MTHFR and MTR genes. Conclusions. Among patients with arthropatic psoriasis, which is the most severe form of psoriasis, the homozygotes for the wild-type allele G of A2756G of MTR gene are more rarely, homozygotes of the TT of C667T of MTHFR gene are found more common than among psoriasis vulgaris patients, which may indicate the contribution of other genes to the development of arthropatic psoriasis.

Keywords: psoriasis, arthropatic psoriasis, folate cycle, MTHFR gene, MTR gene.
Features of the molecular expression of alkaline phosphatase gene in chronic myeloid leukemia
Authors: Shvachko L. P. , Kravchuk I. V. , Teleheiev G. D.
Published: July 2020

Aim. To study expression of alkaline phosphatase gene in chronic myeloid leukemia. Methods. Short-term primary cell culture, real-time PCR. Results. We have investigated the aberrant expression type ALP in CML progression. We have found that the intestinal type ALP is switched to germinal type embrional-like ALP in the patients with clinical CML progression (n=8) resulted from the sequencing analyse. Moreover, we have shown the interconnected epigenetic regulation of aberrant embrional-like type ALP and G-CSFR (granulocyte colony stimulation factor receptor) on the level of mRNA gene expression in CML patients under sodium butirate (HDACi) culture blood treatment (10 мМ). We have revealed simultaneously the epigenetic down-regulation mRNA gene expression of aberrant germinal GALP and up-regulation of G-CSFR by sodium butirate in CML progression. Conclusions. On the data obtained have been concluded that sodium butirate targeted induces the hemopoietic differentiation blood cell potential in CML pathogenesis by the G-CSF signaling pathway activation.

Keywords: CML, alkaline phosphatase, G-CSFR (granulocyte colony stimulation factor receptor), sodium butirate.
The action of extracts of oyster mushrooms and plant collection on survival and the average life expectancy of irradiated mice
Authors: Shamilov E. N. , Abdullaev A. S. , Azizov I. V.
Published: July 2020

Aim. The aim of this work was to evaluate the effect of extracts obtained from oyster mushroom (Pleurotus ostreatus) and vegetable collection, consisting of marigold flowers (Calendula officinalis), St. John's wort (Hypericum perforatum) and yarrow (Achillea millefolium) on survival and the average life expectancy of irradiated mice. Methods. Medicinal plants included in the collection and of oyster mushrooms were dried, and were obtained the extract of water-alcohol mixture. Extracts vnutribryushinno was administered to mice at a concentration of 250 mg/kg 30 min before irradiation at a dose of 9 Gy. Results. It is found that the introduction of the extract of the oyster mushroom there is a significant increase of life expectancy of lethally irradiated animals, although the survival rate after 30 days is slightly inferior to that of the introduction of the radioprotector of the standard. In the case of a complex extract also increase the duration of life of irradiated animals, however, the percentage of survival after the end of the experiment – 30 days – less oyster extract. Conclusions. The data obtained indicate the prospects of using oyster extract separately and in com-plex with extracts of calendula, St. John's wort and yarrow in the development of pharmaceutical compositions intended for the prevention of local radiation injuries.

Keywords: mouse, survival, irradiation, extract.
The Construction of Ideology in Political Discourse: A Deictic Analysis
Authors: Yahya Abdul Aleem
Published: July 2020

This research is conducted on political discourse of a high profile Pakistani politician and a former famous cricketer Imran Khan in the context of Pakistani politics. It aims to understand the ideological process and project of creating a New Pakistan (Naya Pakistan), and that how this project influences the way Imran Khan shapes the political reality during his speeches. The analysis reveals that Imran Khan indexes more often his personal identity as a strong leader of the Tahreek-e-Insaaf party rather than the common or national identity. The results assert this point because the transformation of the country may seem to be only possible under his identity as a leader of Tahreek-e-Insaaf. From the spatial deixes analysis, it manifests that Imran Khan wants to reach to the ideological space or destination of New Pakistan where everyone will have equal rights. This projection of New Pakistan is presented like a utopian world where all things would be right and there would be justice, cooperation and peace. The deixes such as ‘here’, ‘now’ and ‘today’ represent Old Pakistan (Purana Pakistan) which is its present state. But the future along the temporal axes is full of hope that reflects the vision of the founding father of Pakistan ‘Quaid-e-Azam’ in the form of New Pakistan. A comparative study of various other politicians may bring forth further elicitation of political discourse in Pakistani context in future.
Recognition and Actualization of Cohesive Actors in English Arabic Translation
Authors: Hassan A.J.
Published: July 2020

This work assumes that the in cohesiveness of some Arabic translations of typical English texts is the outcome of the mismanagement of four interrelated actors at the sentential and textual levels. These are text structure, connectivity, word choice and reordering. It proposes a composite approach showing the need to observe, acknowledge and/or realize the achievement of two or more of these actors at least. To prove that, a number of various and different translators (15) were given a typical English text to translate into Arabic. It has been shown throughout the analysis that most of them ignore or unknowingly select one or more irrelevant factors and the result is incohesive translated text. This work helps student to observe most if not all instrumental aspects of text cohesion in Arabic text .
Critical Thinking Perspective in ESL New English Textbooks: A Case Study of Pakistan
Authors: Alfadda Hind , Fatima Maryam , Ghaffar Abdul , Afzaal Muhammad
Published: July 2020

This study examines ESL textbooks of English language of grade 9th and 10th of Punjab model schools in Pakistan. In order to achieve the goals of research, the qualitative method was used and applied Paul Elder’s Critical Thinking Model (2008) on the text books of grades 9 and 10. The collected data was analyzed qualitatively. Activities mentioned in the textbooks of grades 9 and 10 of Punjab textbook board were extracted and analyzed in the light of Paul and Elder’s CT model. ESL teachers’ interviews regarding the said textbooks serve as an insight into the role of critical thinking among learners. Mention the period of analysis. There relevance exists between the exercises given in English textbooks with the model of critical thinking prosed by Paul and Elder. However, the teachers of Government schools are unaware of the critical thinking perspective and some sessions of critical thinking training need to be offered to the English school teachers of Government schools. The results of the study have significant implications for material developers. The study recommends that the Government of Punjab may provide a training program to the teachers who may get some sort of training and implement on the students the best of teaching practices in Pakistan. In the same context, the analysis of interviews shows that teachers have been playing a passive role in the promotion of CT in the teaching process. Hence, there is a need to organize some training sessions to make them aware of the basic concept and importance of CT in Pakistan.
Soliciting Audience’s Ovation: The Antagonist’s Artifices and Acting Ingenuity in Shakespeare’s Othello
Authors: Wajih J. Alyo Mohammad
Published: July 2020

Rarely does Shakespeare assign the antagonists in his plays such dominant and pivotal roles as he does in Othello. Seldom, either, does a Shakespearean character exhibit such an obsession with playacting and theatricals as Iago does. The paper at hand explores the consequences of Shakespeare’s unusual decision to tip the traditional balance between protagonist and antagonist in favour of the latter in this great tragedy. The paper argues that Othello is more a play about the splendour of playacting and the charm of actors than it is about evil and evildoing. Arguably devised as suffering from ‘histrionic and narcissistic personality disorders,’ Iago is self-urged, by his latent desire for attention and approval, to parade his histrionic flairs and procure audience’s admiration and commendation. The paper, therefore, assesses the acting and theatrical potentials that Shakespeare invested in the character of Iago, with special attention given to the queer antagonist-audience rapport in the play. The paper concludes that the character of the antagonist is the central attraction in Othello and a major factor in its popular reception. Shakespeare makes Iago ‘the acting dramatist,’ who combines the roles of playwright, actor, stage-manager, and director, to extol the acting profession and emphasise the power of great actors in his day. The paper invites readers of Othello to view Iago not merely as a malefactor but also as a deft actor.
Reading and Listening in English Language Learning: A Critical Study of Cognition and Metacognition
Authors: Al Sobaihi Mohammed
Published: July 2020

This paper critically examines research in the field of cognitive and metacognitive strategies in teaching/learning reading and listening in the English language. In the FEL context, a thorough review of previous major studies has been conducted to determine the extent to which English learners benefit from reading and listening via using the said strategies. The results confirm the effectiveness of cognitive and metacognitive strategies in teaching/learning reading and listening skills and it is recommended that the educational policymakers include them in designing future pedagogical EFL programs.
Variety of Indonesian Dialect in Percut Sei Tuan District of North Sumatera- Indonesia
Authors: Dalimunte Muhammad , Lubis Yani
Published: July 2020

A dialect can effect how a word prounounced diffferently. This study aimed to see how local languages affect Indonesian dialects among people who are still actively using their local languages such as Javanese language, Batak mandailing language, Batak Toba language and Malay. They live in Percut Sei Tuan District of North Sumatra. The research used Qualitative approach. The Informants of the research were taken based on the research data needed. The informants were given a list of 200 vocabularies to be pronounced taken from theory of Swadesh. it was obtained with various dialects in pronouncing indonesian words, there are 161 vocabularies that pronounced diffenerently by the informants but it was still able to understand. The local languages have effects on pronouncing Indonesian word. The diffenrent pronounciation was categorized as subdialect difference. it was at the level of 37,5 (31% - 50% = considered subdialect difference).
Tahmima Anam’s A Golden Age: A Family Saga of Love, Duty and Identity against the Backdrop of War
Authors: Moniruzzaman Miah Mohammad
Published: July 2020

The paper explores love and duty as the dominant themes in Tahmima Anam’s debut novel A Golden Age (2007). At various points of the story, it seems that both love and duty come at the  forefront in turn overshadowing the significance of the other. However, it is not hard to notice that the yearning of love and the call of duty almost equally pervade the story. While analyzing  these compelling themes, the paper critically examines how Anam aesthetically mediates the conflict  between the immediacy of love and the urgency of duty at a time of armed national struggle. It also  emphasizes the status of women in pre-independence Pakistan era male dominated Bengali society and their struggle against various misogynistic norms portrayed through the defiant activities of the  female protagonist in the novel. Apart from defending her feminine identity against the odds of  contemporary androcentric society, the paper also focuses on the issue of the protagonist’s  non-native past and tends to establish her Bangladeshi national identity even though she comes from a different ethnic, lingual and cultural background.
A Pragma- Stylo –Semanitc Analysis of Three Translations of the Meanings of Surratt Al-Saffat into English: A Comparative Linguistic Study
Authors: Mohammed Alhaj Ali Albashir
Published: July 2020

The current study aims at exploring the pragma- stylo-semantic obstacles encounter the translators of the meaning of the Holy Qura’n into English and challenging task in translating Surah Al-Saffat into English as well ; that is in three selected translations of Mohammed A.S Abdel- Hakeem, Mohammed M. Pickthall and Mohammed Khan and Mohammed Taj Al-Din Al-Hilai. Also, the study aims at investigating how the three translators deal with the linguistic, cultural and stylistic, pragmatic difficulties in their translations of Surah Al-Saffat into English.. Ten ayahs from the intended Surah were purposefully selected to address the research questions. The study results reveal that loss in a pragma- stylo –semanitc meaning of Surah Al-Saffat into English occurred due to many factors such as lack of equivalence and the translation strategies employed by the three translators . As far as the strategies adopted in the translations of Surah Al-Saffat is concerned, it is clear that between the two, Abdel-Haleem’s translation is better than Pickthall’s in the sense that it is more informative. Moreover, the study also showed that literal translation poses problems on different levels. These are; word, idiom, style and culture.This study also suggests solutions for the identified pragma- stylo –semantic problems and also recommends for further studies.
High‐resolution crystal structures of the botulinum neurotoxin binding domains from subtypes A5 and A6
Authors: Davies Jonathan R. , Britton Amy , Liu Sai Man , Acharya K. Ravi
Published: July 2020

Clostridium botulinum neurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcholine release from motor neurons; however, at tiny doses, this property is exploited for use as a therapeutic. Each member of the BoNT family of proteins consists of three distinct domains: a binding domain that targets neuronal cell membranes (HC), a translocation domain (HN) and a catalytic domain (LC). Here, we present high‐resolution crystal structures of the binding domains of BoNT subtypes/A5 (HC/A5) and/A6 (HC/A6). These structures show that the core fold identified in other subtypes is maintained, but with subtle differences at the expected receptor‐binding sites.
Does state self‐control depletion predict relationship functioning and partner aggression? An ecological momentary assessment study of community couples
Authors: Testa Maria , Wang Weijun , Derrick Jaye L. , Crane Cory , Leonard Kenneth E. , Collins R. Lorraine , Hanny Courtney , Muraven Mark
Published: July 2020

Intimate relationship functioning depends upon the ability to accommodate one's partner and to inhibit retaliatory and aggressive impulses when disagreements arise. However, accommodation and inhibition may be difficult when self‐control strength is weak or depleted by prior exertion of self‐control. The present study considered whether state self‐control depletion prospectively predicts male and female self‐reports of anger with partner and arguing with partner. Consistent with the I3 Model (Finkel, 2014, Adv Exp Soc Psychol, 49, 1–104), we also considered whether the association between elevated anger and arguing (i.e., instigation) and partner aggression was stronger when state self‐control (i.e., inhibition) was depleted or among people high in negative urgency. In this ecological momentary assessment (EMA) study, heavy drinking married and cohabiting heterosexual couples (N = 191) responded to three randomly signaled reports each day for 30 days. Depletion predicted anger and arguing with partners both cross‐sectionally and prospectively for men and women. However, after controlling for prior levels of anger and arguing, these effects were diminished, and supplemental analyses revealed that anger and arguing with partner predicted subsequent depletion. Anger and arguing were strongly associated with concurrent reports of partner aggression perpetration and victimization (verbal and/or physical). However, neither state self‐control depletion nor negative urgency moderated these effects. Overall, results suggest a modest impact of depletion on daily couple functioning as well as a potential cyclical effect of arguing on depletion.
Extremely Tough Hydrogels with Cotton Fibers Reinforced
Authors: Zhang Ran , Wu Yang , Lin Peng , Jia Zhengfeng , Zhang Yujue , Liu Fengzhen , Yu Bo , Zhou Feng
Published: July 2020

Creating both strong and tough hydrogel materials have been a challenging task, due to their nature of generally mutual exclusion. Interestingly, natural materials such as bone and shells usually offer an unprecedented combination of strength and toughness, which would provide inspiration for novel materials. Herein, the design of natural cartilage is mimicked with collagen fibers incorporated into extracellular matrix, and fabric‐reinforced composite hydrogels are prepared. The poly (vinyl alcohol) (PVA) hydrogel composite exhibits significant improvement in the mechanical properties, compared with cotton scaffold or neat PVA gel. Under deformation, the fibers in the hydrogel network are pulled out and break up gradually to dissipate energy, providing significant stiffness and toughness for the gel composite. This work opens up a novel and simple method to construct tough materials and displays potential application in joint‐replacement.
Free‐Standing Single‐Walled Carbon Nanotube/SnSe Nanosheet/Poly(3,4‐Ethylenedioxythiophene):Poly(4‐Styrenesulfonate) Nanocomposite Films for Flexible Thermoelectric Power Generators
Authors: Liu Xin , Du Yong , Meng Qiufeng , Dou Yunchen , Jin Min , Xu Jiayue , Shen Shirley Z.
Published: July 2020

Flexible single‐walled carbon nanotube (SWCNT)/SnSe nanosheet (NS)/poly(3,4‐ethylenedioxythiophene):poly(4‐styrenesulfonate) (PEDOT:PSS) nanocomposite films are first successfully prepared by a facile vacuum filtration method on nylon membrane. An optimized power factor of 109.9 μWm−1 K−2 for the as‐prepared free‐standing nanocomposite film with 60 wt% SWCNTs is obtained at the temperature of 380 K, and the electrical conductivity only decreases by 1% after 1000 bending cycles (the bending radius is ≈4.3 mm), indicating excellent flexibility. A flexible thermoelectric generator consisting of 8‐unit free‐standing composite film strips (25 mm × 4 mm for each strip) is assembled on a polyimide substrate, which can generate an open‐circuit voltage of 20.6 mV and a maximum output power of 1.16 μW at a temperature gradient of 74.4 K. This work offers quite an effective strategy for preparation of high‐performance flexible free‐standing thermoelectric composite films, and the as‐prepared films can avoid excess heat loss induced by the substrate‐adherent layer of thin films, when compared with the traditional thermoelectric composite films prepared on different kinds of substrates, e.g., glass substrate. Therefore, the composite films have a great potential to be used for the flexible thermoelectric devices.
SAM Functionalized ZnO Nanowires for Selective Acetone Detection: Optimized Surface Specific Interaction Using APTMS and GLYMO Monolayers
Authors: Singh Mandeep , Kaur Navpreet , Drera Giovanni , Casotto Andrea , Sangaletti Luigi , Comini Elisabetta
Published: July 2020

In modern days, self‐assembled monolayer (SAM) functionalized surfaces represent an interesting tool for the development of ultrasensitive and selective sensing platforms for the detection of chemical substances such as biomolecules and gases. The ability of SAM to generate different functional groups on a single surface such as zinc oxide (ZnO) can be used to immobilize biomolecules and detect different analytes such as gases, proteins, etc. Herein, SAM functionalized ZnO NW‐based sensors are developed for acetone exhaled breath analysis. ZnO NWs are synthesized using a vapor–liquid–solid mechanism and their functionalization is done with two different SAMs, i.e., (3‐aminopropyl)trimethoxysilane (APTMS) and 3‐glycidoxypropyltrimethoxysilane (GLYMO). The enhancement in the electron depletion layer resistance (and also width) due to the capturing of electrons from the ZnO NWs surface by APTMS and GLYMO molecules is found to be the major reason in their superior sensing performances. The amine (–NH2) groups of APTMS monolayer enhance the sensors selectivity toward acetone due to their reactions with acetone molecules, which produce imine in addition to water molecules. Moreover, after the functionalization with APTMS SAMs, the detection limits of the sensors are improved from 6 to 0.5 ppm, which makes these devices potential candidates for acetone exhaled breath analysis.
Integrated process design, scheduling, and model predictive control of batch processes with closed‐loop implementation
Authors: Burnak Baris , Pistikopoulos Efstratios N.
Published: July 2020

Simultaneous evaluation of multiple time scale decisions has been regarded as a promising avenue to increase the process efficiency and profitability through leveraging their synergistic interactions. Feasibility of such an integral approach is essential to establish a guarantee for operability of the derived decisions. In this study, we present a modeling methodology to integrate process design, scheduling, and advanced control decisions with a single mixed‐integer dynamic optimization (MIDO) formulation while providing certificates of operability for the closed‐loop implementation. We use multi‐parametric programming to derive explicit expressions for the model predictive control strategy, which is embedded into the MIDO using the base‐2 numeral system that enhances the computational tractability of the integrated problem by exponentially reducing the required number of binary variables. Moreover, we apply the State Equipment Network representation within the MIDO to systematically evaluate the scheduling decisions. The proposed framework is illustrated with two batch processes with different complexities.