bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementation mapping for tobacco cessation in a federally qualified health center
Authors: Domlyn Ariel M. , Crowder Carolyn , Eisenson Howard , Pollak Kathryn I. , Davis James M. , Calhoun Patrick S. , Wilson Sarah M.
Published: September 2022

Background

Implementation mapping (IM) is a promising five-step method for guiding planning, execution, and maintenance of an innovation. Case examples are valuable for implementation practitioners to understand considerations for applying IM. This pilot study aimed to determine the feasibility of using IM within a federally qualified health center (FQHC) with limited funds and a 1-year timeline.

Methods

An urban FQHC partnered with an academic team to employ IM for implementing a computerized strategy of tobacco cessation: the 5A's (Ask, Advise, Assess, Assist, Arrange). Each step of IM was supplemented with theory-driven methods and frameworks. Data collection included surveys and interviews with clinic staff, analyzed via rapid data analysis.

Results

Medical assistants and clinicians were identified as primary implementers of the 5A's intervention. Salient determinants of change included the perceived compatibility and relative priority of 5A's. Performance objectives and change objectives were derived to address these determinants, along with a suite of implementation strategies. Despite indicators of adoptability and acceptability of the 5A's, reductions in willingness to adopt the implementation package occurred over time and the intervention was not adopted by the FQHC within the study timeframe. This is likely due to the strain of the COVID-19 pandemic altering health clinic priorities.

Conclusions

Administratively, the five IM steps are feasible to conduct with FQHC staff within 1 year. However, this study did not obtain its intended outcomes. Lessons learned include the importance of re-assessing barriers over time and ensuring a longer timeframe to observe implementation outcomes.
Clinical significance and role of coinfections with respiratory pathogens among individuals with confirmed severe acute respiratory syndrome coronavirus-2 infection
Authors: Trifonova Ivelina , Christova Iva , Madzharova Iveta , Angelova Svetla , Voleva Silvya , Yordanova Ralitsa , Tcherveniakova Tatiana , Krumova Stefka , Korsun Neli
Published: September 2022

Introduction

This study aimed to determine the prevalence, viral profile, and clinical features of coinfections with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and other respiratory viruses.

Methods

Nasopharyngeal samples and clinical data of 221 hospitalized patients and 21 outpatients were collected and analyzed. Real-time reverse transcription-polymerase chain reaction was used to detect SARS-CoV-2, influenza virus, respiratory syncytial virus (RSV), human metapneumovirus (HMPV), parainfluenza virus (PIV) 1,2,3, rhinovirus (RV), adenovirus (AdV), bocaviruses (BoV), and seasonal coronaviruses (OC43, 229E, NL63, and HKU1). Viral load was determined by capillary electrophoresis.

Results

From November 2020 to mid-March 2022, 242 SARS-CoV-2 positive patients were tested for seasonal respiratory viruses, and 24 (9.9%) cases of coinfections were detected. The distribution of viruses involved in cases of coinfections were as follows: HMPV (n = 6; 25%), RSV (n = 4;16.7%), AdV (n = 4; 16.7%), BoV (n = 4; 16.7%), PIV3 (n = 2; 8.3%), influenza A (H3N2; n = 2; 8.3%), RV (n = 1; 4.62%), and RV+BoV (n = 1; 4.62%). The proportion of detected coinfections with SARS-CoV-2 was highest in children aged 0–5 years (59%), followed by those >65 years (33%). In specimens with detected coinfection, the viral load of influenza was higher than that of SARS-CoV-2, and the mean viral load of SARS-CoV-2 was higher than that of the other respiratory viruses. C-reactive protein (CRP) and lymphocytes count in co-infected patients >65 years of age were on average higher than in children <16 years of age (mean CRP of 161.8 ± 133.1 mg/L; 19.7 ± 3.09% vs. mean 6.9 ± 8.9 mg/L, 0.9 ± 3.1%; p < 0.01). Patients >65 years of age co-infected with SARS-CoV-2 and other respiratory viruses had longer hospital stays than those <16 years of age (mean 9 ± 3.96 days vs. 5.44 ± 1.89 days; p = 0.025). The combination of AdV and SARS-CoV-2 is fatal for patients aged >65 years.

Conclusion

In patients aged >65 years, coinfection with SARS CoV-2 and other respiratory viruses, together with concomitant diseases, causes worsening of the clinical picture and complications, and can be fatal. Screening of patients with SARS CoV-2 for other respiratory viruses is needed to select appropriate treatments and prevent a fatal outcome of the disease.
Oral microbial taxa associated with risk for SARS-CoV-2 infection
Authors: Callahan Nicholas , Hattar Meryana , Barbour Thawab , Adami Guy R. , Kawar Nadia
Published: September 2022

Hypothesis and objective

The oral and digestive tract microbial ecosystem has sparked interest because of its impact on various systemic diseases and conditions. The oral cavity serves not only as a reservoir for many potentially virulent microbiota but also as an important entry point and portal to the human body system. This is especially significant in the transmissibility of the virulent current pandemic virus SARS-CoV-2. The oral and digestive microbiome influences the inflammatory burden and effectiveness of the immune system and serves as a marker of activity of these host processes. The host immune response plays a role in infection susceptibility, including SARS-CoV-2. The purpose of this study is to investigate the role of specific salivary oral microbiome in susceptibility to SARS-CoV-2 infection.

Methods and results

One hundred six subjects of known medical and dental history who consented to provide saliva samples between January 2017 and December 2019 were included in this study. Sixteen had become COVID-19 positive based on the PCR test by 3/01/2021. A comparison of oral microbiome bacteria taxa profiles based on 16S rRNA sequencing revealed differences between the two groups in this pilot study.

Conclusions

These bacteria taxa may be markers of increased susceptibility to SARS-CoV-2 infection in the unvaccinated population.
Heterofermentative lactic acid bacteria such as Limosilactobacillus as a strong inhibitor of aldehyde compounds in plant-based milk alternatives
Authors: Sugahara Hirosuke , Kato Sayaka , Nagayama Keitaro , Sashihara Koichi , Nagatomi Yasushi
Published: September 2022

Reduction of greenhouse gas emissions is important to limit climate change. Because ruminant animals emit greenhouse gases, the worldwide plant-based alternative market is an emerging trend for eating less meat and dairy products. To produce plant-based dairy products such as yogurt alternatives, certain lactic acid bacterial species, which are used for cow's milk fermentation, are often used. Substrate changes from cow's milk to plant-based milk caused nutritional changes, and unsaturated fatty acids are more enriched in plant-based milk alternatives than in cow's milk. Unsaturated fatty acids can lead to the formation of aldehydes, some of which are off-flavors; therefore, substrate changes have the potential to alter the suitable lactic acid bacterial species used for fermentation to control flavor formation, such as aldehyde compounds. However, differences in the effect of the fermentation processes on aldehyde compounds have not been evaluated among lactic acid bacterial species. In this study, we comprehensively evaluated the effect of lactic acid bacterial fermentation on aldehyde compounds in synthetic medium and plant-based milk alternatives using 20 species of lactic acid bacteria. Heterofermentative lactic acid bacteria such as strains belonging to Limosilactobacillus had a strong aldehyde-reducing ability, likely from differences in alcohol dehydrogenase function. Because the odor detection threshold of ethanol compounds was lower than that of their equivalent aldehyde compounds, our findings are valuable for the fermentation of plant-based milk alternatives with lactic acid bacteria with the goal of decreasing off-flavors derived from aldehyde compounds.
Entomopathogenic fungi as biological control agents of Dactylopius opuntiae (Hemiptera: Dactylopiidae) under laboratory and greenhouse conditions
Authors: Ramdani Chaimae , El Fakhouri Karim , Boulamtat Rachid , Bouharroud Rachid , Mesfioui Abdelhalim , Al-Jaboobi Muamar , El Bouhssini Mustapha
Published: September 2022

The cactus Opuntia ficus-indica L. is widly cultivated in Morocco and has a very an important economic role as a source of food, livestock feed and not forgetting that it is considered to be an income for people in rural communities. This crop is subject to the attack of a serious insect pest, the Dactylopius opuntiae (Cockerell), which sucks the sap from the plant causing huge production losses since its introduction to the country in 2014. The present study investigated the entomopathogenic effect of six fungal isolates {Beauveria bassiana [HASS; RFSL10; SPT 011(a)]; Akanthomyces lecanii [RFSLV; SPT R 215] and Cordyceps farinosa [SPSBI4]} for controlling of both nymphs and adult females of D. opuntiae in laboratory and greenhouse bioassays. Under laboratory conditions, the highest mortality of female D. opuntiae was registered by B. bassiana strain HASS at 108 conidia ml−1 with 100%, followed by B. bassiana strain RFSL10, A. lecanii RFSLV, and C. farinosa SPSBI4 isolates with 98%, respectively, 10 days after treatments. Similarly, the highest level of nymph mortality (100%) was recorded by B. bassiana RFSL10 and HASS isolates at 108 conidia ml1, respectively, 4 and 5 days after application. Under greenhouse conditions, B. bassiana (HASS and RFSL10) and A. lecanii (RFSLV) isolates sprayed alone expressed a higher toxicity on nymphs with 75, 68.5 and 58%, respectively, 12 days after treatments. However, no significant difference was observed in adult female's mortality between different fungal isolates, where B. bassiana (HASS) at 108 conidia ml−1 presented a moderate mortality rate with 55%, 12 days after application. In fact, the combination of black soap (60 g/L) with B. bassiana HASS and RFSL10 and A. lecanii (RFSLV) isolates at 108 conidia ml−1 caused the highest toxic activity on D. opuntiae adult females, with 70.5, 68.75 and 67.65%, respectively. These findings showed that entomopathogenic fungi are promising for developing a biopesticide formulation for the management of D. opuntiae as an adequate and safe alternative to chemical pesticides.
Callispheres drug-eluting bead transhepatic artery chemoembolization with oral delivery of sorafenib for the treatment of unresectable liver cancer
Authors: Wang Wenhui , Li Fenqiang , Gan Peiying , Li Baohua , Li Shuangxi
Published: September 2022

Objective

Liver cancer is a significant contributor to global burden of cancer. Transcatheter arterial chemoembolization (TACE) is the standard of care for patients with unresectable liver cancer, and CalliSpheres, as novel drug-eluting bead (DEB) microspheres, have been found to be associated with a high tumor response rate. However, the outcomes after DEB-TACE treatment are not always satisfactory with tumor recurrence. Herein, we attempt to compare the clinical efficacy and safety of DEB-TACE with sorafenib and conventional TACE in treating advanced liver cancer.

Methods

The study retrospectively reviewed clinical records of 96 patients with liver cancer, among which there were 48 cases receiving DEB-TACE with sorafenib and 48 cases receiving conventional TACE. The physical properties of Callispheres were evaluated in HepG2 cells and a B6/J mouse model.

Results

DEB-TACE with Callispheres were demonstrated to effectively maintain stability and prolong the half-life of epirubicin. Compared with the patients receiving conventional TACE, those receiving DEB-TACE with sorafenib exhibited better patient outcomes with increased survival rate, reduced tumor volume, and declined levels of tumor markers. Additionally, DEB-TACE with Callispheres could effectively protect liver function, as well as reduce the toxic effects of loaded epirubicin, and its combination with sorafenib would not increase the incidence of adverse reactions.

Conclusion

DEB-TACE using CalliSpheres combined with sorafenib could prevent the progression of liver cancer and bring a better prognosis.
Current Status and research hotspots in the field of full endoscopic spine surgery: A bibliometric analysis
Authors: Lin Guang-Xun , Zhu Ming-Tao , Kotheeranurak Vit , Lyu Pengfei , Chen Chien-Min , Hu Bao-Shan
Published: September 2022

Purpose

We aimed to comprehensively analyze the current status, hotspots, and trends in full endoscopic spine surgery (FESS) research using bibliometric analysis and knowledge domain mapping.

Methods

The Web of Science database was used to screen FESS-related articles published between January 1, 1993 and June 10, 2022. The evaluation involved the following criteria: total number of articles; H-index; and contributions from countries/regions, institutions, journals, and authors.

Results

A total of 1,064 articles were included. Since 2016, there have been a significant number of publications in the field of FESS. The country/region contributing the largest number of articles was China (37.8%), followed by South Korea (24%), the United States (16.1%), Japan (5.7%), and Germany (5.1%). South Korea (35) had the highest H-index, followed by the United States (27), China (22), Japan (21), and Germany (20). World Neurosurgery (15.7%) published the largest number of FESS-related articles. However, among the top 10 most cited articles, six were published in Spine. The author who contributed the most was S.H. Lee (5.4%), and the largest number of contributions in this field originated from Wooridul Spine Hospital (South Korea; 6.1%). Notably, six of the 10 most published authors in this field were from South Korea. Of the top five productive institutions, three were from South Korea. The keywords with the strongest citation bursts in the field of FESS were “lumbar spine,” “discectomy,” “interlaminar,” “surgical technique,” “follow-up,” “excision,” “thoracic spine,” and “endoscopic surgery.” The 10 clusters generated in this study were: “endoscopic discectomy” (#0), “thoracic myelopathy” (#1), “recurrent lumbar disc herniation” (#2), “low back pain” (#3), “cervical vertebrae” (#4), “lumbar spinal stenosis” (#5), “transforaminal lumbar interbody fusion” (#6), “radiation exposure” (#7), “management” (#8), and “lumbar spine” (#9).

Conclusion

Global research on FESS is mostly concentrated in a few countries/regions and authors. South Korea has made the largest contribution to the field of FESS. Based on the most cited keyword bursts and clusters, the focus of FESS research was found to include its indications, management, and applications.
Quantifying canine interactions with smart toys assesses suitability for service dog work
Authors: Byrne Ceara , Starner Thad , Jackson Melody
Published: September 2022

There are approximately a half million active service dogs in the United States, providing life-changing assistance and independence to people with a wide range of disabilities. The tremendous value of service dogs creates significant demand, which service dog providers struggle to meet. Breeding, raising, and training service dogs is an expensive, time-consuming endeavor which is exacerbated by expending resources on dogs who ultimately will prove to be unsuitable for service dog work because of temperament issues. Quantifying behavior and temperament through sensor-instrumented dog toys can provide a way to predict which dogs will be suitable for service dog work, allowing resources to be focused on the dogs likely to succeed. In a 2-year study, we tested dogs in advanced training at Canine Companions for Independence with instrumented toys, and we discovered that a measure of average bite duration is significantly correlated with a dog's placement success as a service dog [Adjusted OR = 0.12, Pr(>|z|) = 0.00666]. Applying instrumented toy interactions to current behavioral assessments could yield more accurate measures for predicting successful placement of service dogs while reducing the workload of the trainers.
Characteristics of thermal images of the mammary gland and of performance in sows differing in health status and parity
Authors: Rosengart Stephan , Chuppava Bussarakam , Trost Lea-Sophie , Henne Hubert , Tetens Jens , Traulsen Imke , Deermann Ansgar , Wendt Michael , Visscher Christian
Published: September 2022

Precision livestock farming can combine sensors and complex data to provide a simple score of meaningful productivity, pig welfare, and farm sustainability, which are the main drivers of modern pig production. Examples include using infrared thermography to monitor the temperature of sows to detect the early stages of the disease. To take account of these drivers, we assigned 697 hybrid (BHZP db. Viktoria) sows to four parity groups. In addition, by pooling clinical findings from every sow and their piglets, sows were classified into three groups for the annotation: healthy, clinically suspicious, and diseased. Besides, the udder was thermographed, and performance data were documented. Results showed that the piglets of diseased sows with eighth or higher parity had the lowest daily weight gain [healthy; 192 g ± 31.2, clinically suspicious; 191 g ± 31.3, diseased; 148 g ± 50.3 (p< 0.05)] and the highest number of stillborn piglets (healthy; 2.2 ± 2.39, clinically suspicious; 2.0 ± 1.62, diseased; 3.91 ± 4.93). Moreover, all diseased sows showed higher maximal skin temperatures by infrared thermography of the udder (p< 0.05). Thus, thermography coupled with Artificial Intelligence (AI) systems can help identify and orient the diagnosis of symptomatic animals to prompt adequate reaction at the earliest time.
Incidence and concomitant factors of cesarean sections in the bitch: A questionnaire study
Authors: Schrank Magdalena , Contiero Barbara , Mollo Antonio
Published: September 2022

Dystocia in the canine species is a common problem, and elective cesarean sections (C-sections) have become more frequent in breeds that are at risk. The aim of this study was to evaluate the incidence of C-section and contributing factors and to compare data on elective and emergency C-sections (e.g., regarding stillbirth). Using a questionnaire, a total of 423 bitches of 80 breeds and their 899 litters were included. The mean number of litters per bitch was 2.1 ± 1.1 litters. The overall rate of stillbirth was 6.7%. Of all litters, 194 were born via C-sections (21.6%), of which 35 were declared as elective and 159 as emergency due to dystocia. Significantly more C-sections were performed in either small litters (1-2 pups) or large litters (>12 pups) (p < 0.001). Bitches that have had prior C-sections had a 4-fold increase in the risk of successive C-sections (RR = 4.54 (95%CI 2.56–7.70; p < 0.001). Furthermore, primiparous bitches of advanced age had a significantly higher incidence of emergency C-sections (p = 0.004). Stillbirth was significantly higher in emergency C-sections compared with that in elective C-sections (p = 0.003). Also, timing of intervention had a significant impact on stillbirth in emergency C-sections (p = 0.025). Within a breed-specific evaluation, significant differences were observed between breeds regarding incidence of C-section and stillbirth. Lesser-known breeds were represented in the population, and the results showed that the Norwich Terrier had the highest (51.6%) and the Gordon Setter had the lowest (4.8%) incidence of C-section (p < 0.001). The inclusion and evaluation of lesser-known breeds regarding incidence of C-section is of importance as it shows that certain breeds without phenotypical traits such as brachycephaly may also have an increased incidence of emergency C-section and stillbirth. We further conclude that more importance may be given to the age at first parturition concerning the occurrence of dystocia and the decision making regarding possible elective C-sections.
Plasma atropine concentrations associated with decreased intestinal motility in horses
Authors: Ekstrand Carl , Michanek Peter , Gehring Ronette , Sundell Anna , Källse Annika , Hedeland Mikael , Ström Lena
Published: September 2022

Introduction

Atropine is an essential part of the treatment protocol for equine uveitis. Topical atropine administration has been associated with decreased intestinal motility and abdominal pain in horses. Experimental studies have indicated that frequent dosing is associated with a higher risk than dosing every 6 h. Unfortunately, no quantitative pharmacodynamic data for inhibition of the equine gut are published.

Materials and methods

Eight standardbred horses were assigned to receive either atropine or saline (control) to be infused over 30 min in a two-treatment cross-over design. Atropine concentrations in plasma were measured using ultra-high-performance liquid chromatography–tandem mass spectrometry. Intestinal motility was measured using borborygmi frequency and electrointestinography (EIG). Experimental data were analyzed using a non-linear mixed effects model. The model was then used to simulate different dosing regimens.

Results

Atropine significantly decreased borborygmi response and EIG response. Six horses developed clinical signs of abdominal pain. The pharmacokinetic typical values were 0.31, 1.38, 0.69, and 1.95 L/kg·h for the volumes of the central, the highly perfused, the scarcely perfused compartments, and the total body clearance, respectively. The pharmacodynamic typical values were 0.31 μg/L and 0.6 and 207 nV27 cpm for the plasma concentration at 50% of the maximum response and the maximum response and the baseline of cecal EIG response, respectively. Six different dosing regimens of topical atropine sulfate to the eye (0.4 and 1 mg every hour, every 3 h, and every 6 h) were simulated.

Conclusion

The IV PK/PD data coupled with simulations predict that administration of 1 mg of topical atropine sulfate administered to the eye every hour or every 3 h will lead to atropine accumulation in plasma and decreased intestinal myoelectric activity. Administration every 6 h predicted a safe dosing regimen in full-sized horses. Clinical studies would be valuable to confirm the conclusions. For smaller equids and horses put at risk for colic due to othercauses, droplet bottles that deliver 40 μl of 1% atropine sulfate per drop or less may be used to lower the risk further.
Effects of fulvic acid addition on laying performance, biochemical indices, and gut microbiota of aged hens
Authors: Xiao Gengsheng , Liu Shun , Yan Xia , Yang Yang , Qi Qien , Feng Xin , Gong Li , Zhang Huihua
Published: September 2022

The purpose of this study was to appraise the effect of fulvic acid on production, biochemical indices, and gut microbiota of laying hens. A total of 252 Dawu Golden Phoenix laying hens (55-week-old) were allotted to two treatments randomly, each with six replicates and 21 hens per replicate, including the control group (CG) and fulvic acid (500 mg/kg) group (FA). The trial period was 8 weeks. Adding FA raised egg weight (P = 0.03), shell-breaking strength (P = 0.03), and reduced egg breaking rate (P < 0.01), compared with CG. There was no difference in eggshell thickness and egg shape index between the two treatments; however, the FA group increased egg production by 1.45% and reduced the feed-to-egg ratio by 0.09. Moreover, dietary FA decreased the aspartate aminotransferase levels in serum (P = 0.04), and glutathione peroxidase and total antioxidant capacity were increased (P = 0.02 and 0.04, respectively). Despite this, the two groups had no differences in the alpha diversity indices (PD_whole trees, Shannon, Ace, Simpson, Chao1, and goods_coverage). Obviously, at the phylum level, the abundances of Firmicutes were improved (P < 0.01), Actinobacteriota (P < 0.01), and Proteobacteria (P < 0.01) were reduced by dietary FA. Supplementation with FA could improve the abundances of Megamonas (P < 0.01) and reduce Enterobacter (P < 0.01) at the genus level. To sum up, this study showed the addition of 500 mg/kg FA may boost production and egg quality and modulate the cecal microflora abundance and serum biochemical indices of laying hens.
Effect of dietary β-mannanase supplementation on growth performance, digestibility, and gene expression levels of Cyprinus carpio (Linnaeus) fingerlings fed a plant protein-rich diet
Authors: Dawood Aneesa , Shi Weibin
Published: September 2022

The aim of this study was to assess possible beneficial effects of dietary β-mannanase supplementation on the nutrient digestibility, growth performance, digestive and metabolic enzyme activity, and immune response of common carp (Cyprinus carpio) fed plant protein-rich diets. An experiment was conducted in triplicate, and a total of 225 fingerlings of common carp with an average body weight of 13.17 ± 0.12 g were stocked in 15 fiberglass tanks (15 fish/tank). Five dietary treatments (control 35% crude protein, plant-rich basal diet without supplement and four diets supplemented with β-mannanase from two sources (commercially available and locally isolated), each at two dosage levels (500 and 1,000 U/kg diet) were prepared and fed to respective groups of fish, twice a day (8:00 AM and 4:00 PM) at 4 % body weight. During the trial, changes in the level of DO and temperature ranged from 5.5 to 6.1 mg L−1 and 21.5 to 23.5°C, respectively. At the end of the feeding experiment, all fish in each tank were weighed and counted to determine growth parameters, while for the study of other indices, nine samples/treatment group were selected. The results of the study indicated a positive effect of both sources and dosage levels of β-mannanase supplementation on all studied indices, that is, significantly improved (P < 0.05), growth performance (%weight gain, specific growth rate), survival %, hematological indices (RBC, Hb, HCT, and MCHC), immunological indices (lysozyme activity, WBC, respiratory burst activity, and phagocytic activity), improved apparent digestibility of nutrients (crude protein, crude fat, and carbohydrates), and digestible energy. Furthermore, higher activity (P < 0.05) of the digestive enzymes (cellulase, lipase, and protease) and upregulation of MyoD gene in muscle and TNF-α gene in liver, intestine, and muscle were also observed, while the activity of serum AST (serum aspartate aminotransferase) and ALT (alanine transaminase) as compared to control group was significantly decreased (P < 0.05). Based on the results, β-mannanase supplementation (500 U/kg) could be recommended for obtaining better carp production when low-cost plant protein-rich diets are used.
Establishment methods and research progress of livestock and poultry immortalized cell lines: A review
Authors: Guo Dongxue , Zhang Li , Wang Xiaotong , Zheng Jiahui , Lin Shudai
Published: September 2022

An infinite cell line is one of the most favored experimental tools and plays an irreplaceable role in cell-based biological research. Primary cells from normal animal tissues undergo a limited number of divisions and subculturesin vitrobefore they enter senescence and die. On the contrary, an infinite cell line is a population of non-senescent cells that could proliferate indefinitelyin vitrounder the stimulation of external factors such as physicochemical stimulation, virus infection, or transfer of immortality genes. Cell immortalization is the basis for establishing an infinite cell line, and previous studies have found that methods to obtain immortalized cells mainly included physical and chemical stimulations, heterologous expression of viral oncogenes, increased telomerase activity, and spontaneous formation. However, some immortalized cells do not necessarily proliferate permanently even though they can extend their lifespan compared with primary cells. An infinite cell line not only avoids the complicated process of collecting primary cell, it also provides a convenient and reliable tool for studying scientific problems in biology. At present, how to establish a stable infinite cell line to maximize the proliferation of cells while maintaining the normal function of cells is a hot issue in the biological community. This review briefly introduces the methods of cell immortalization, discusses the related progress of establishing immortalized cell lines in livestock and poultry, and compares the characteristics of several methods, hoping to provide some ideas for generating new immortalized cell lines.
First molecular evidence of Clostridium perfringens in adult Fasciola spp. isolates in cattle hosts
Authors: Karagulle Burcu , Celik Figen , Simsek Sami , Ahmed Haroon , Shen Yujuan , Cao Jianping
Published: September 2022

Fasciolosis is a parasitic disease caused by Fasciola spp. It is a prevalent helminth infection globally. Clostridial hepatitis is a general name refer to disorders caused by a few clostridial agents that most severely affect the liver. Migration of young parasite forms (mostly Fasciola hepatica) in the parenchymal tissue of the liver causes necrosis and anaerobic environment, stimulating the proliferation of C. novyi type B spores. This study investigated the occurrence of Clostridium spp in adult Fasciola spp isolates. Isolates (n = 100) were collected from the bile ducts of infected cattle after slaughter. Total genomic DNA was extracted from each sample. A multiplex-PCR based on the flagellin C (fliC) gene was used for quick identification of C. chauvoei, C. haemolyticum, C. novyi types A and B, and C. septicum. In addition, a pair of primers Cpa (F) and Cpa (R) were used for detection of the C. perfringens alpha toxin gene. The products were sequenced. No band was obtained after multiplex-PCR of the fliC gene. A 247 bp band was detected in two isolates using the Cpa primers. BLAST analysis of these two isolates characterized both as C. perfringens alpha toxin. This is the first description of the molecular detection of C. perfringens in flukes. Further studies are needed to investigate whether Clostridum species is also carried by other developmental forms (egg and larval stages) of Fasciola spp.
Do intense weather events influence dogs' and cats' behavior? Analysis of owner reported data in Italy
Authors: Palestrini Clara , Minozzi Giulietta , Mazzola Silvia Michela , Lopez Annalaura , Cannas Simona
Published: September 2022

Climate change is a threat to global health and can affect both veterinary and human health. Intense weather events, including sudden and violent thunderstorms or periods of extreme heat, are predicted to rise in frequency and severity and this could lead owners to significantly change their habits and schedules based on the weather, could modify human management and could aggravate pre-existing behavioral problems in pets. The aims of the present study were to identify and quantify possible weather events impact on management, behavior, and behavioral problems of Italian dogs and cats, based on previous owners' experiences with their animals. Two questionnaires were prepared, one for dogs and one for cats, investigating owners' perceptions of the impact of weather events on their pets' behavior. A number of 392 dogs and 426 cats' owners answered the questionnaire. Our study showed that many behaviors in both species were equally modified by environmental temperature. Play and activity increased with cold weather and decreased with heat, and sleep increased with drops in temperature and with hot weather. In particular, the increase in activity in correspondence with the thermic drop was more significant in males, while the increase in playing behavior was statistically greater in the Sheepdogs and Cattle dogs –group1. Weather events did not affect aggressive and house soiling behaviors in both dogs and cats, but weather events, including wild thunderstorms, torrential rains influenced the pets' behavior. Understanding how pets modify their behaviors based on a different owners' schedule and to weather events can help to refine prevention strategies through societal changes and owner education.
Overexpression of GPR41 attenuated glucose production in propionate-induced bovine hepatocytes
Authors: Lin Miao , Jiang Maocheng , Yang Tianyu , Zhao Guoqi , Zhan Kang
Published: September 2022

Bovine liver mainly utilizes the propionate as a gluconeogenic substrate to synthesize the glucose. However, the mechanism underlying the regulatory effects of propionate on the glucose production in bovine hepatocytes remains less known. Previous studies have demonstrated G protein-coupled receptor 41 (GPR41) as receptors for propionate. We hypothesized that propionate may regulate the glucose production by GPR41 in bovine hepatocytes. Therefore, the aim of the study was to investigate the regulatory effects of propionate and GPR41 on glucose production in bovine hepatocytes. Hepatocytes with GPR41 overexpression were incubated in the presence of either 0 or 3 mM propionate for 24 h. These results showed that the expression of phosphoenolpyruvate carboxykinase 2 (PCK2) and pyruvate carboxylase (PC) genes involved in gluconeogenesis was enhanced (P < 0.01) with propionate treatment. Remarkably, the addition of propionate promotes the glucose production in bovine hepatocytes. Expression of GPR41 was increased by the addition of propionate in bovine hepatocytes overexpressed GPR41 by overexpression plasmid AAV1 compared with the absence of propionate. Interestingly, expression of PCK2 was markedly attenuated in GPR41 overexpressed-hepatocytes with propionate. Importantly, overexpression of GPR41 attenuated glucose output in propionate-induced bovine hepatocytes. These findings revealed that GPR41 negatively regulates glucose production by downregulating the expression of PCK2 in propionate-induced bovine hepatocytes.
Expression profile of long non-coding RNA in inner Mongolian cashmere goat with putative roles in hair follicles development
Authors: Ma Rong , Shang Fangzheng , Rong Youjun , Pan Jianfeng , Wang Min , Niu Shuran , Qi Yunpeng , Li Yanbo , Lv Qi , Wang Zhiying , Wang Ruijun , Su Rui , Liu Zhihong , Zhao Yanhong , Wang Zhixin , Li Jinquan , Zhang Yanjun
Published: September 2022

The hair follicle is a complex skin accessory organ, which determines hair growth. Long non-coding RNAs (lncRNAs) have been proven to play an important role in hair follicle development, but their specific mechanism is still unclear. In this study, high-throughput sequencing was used to obtain the expression profiles of lncRNA in the hair follicles of Inner Mongolian cashmere goats at different embryonic stages (45, 55, 65, and 75 days), and a total of 6,630 lncRNA were identified. According to the rules of hair follicle development, we combined miRNA and mRNA databases (published) and predicted lncRNA-miRNA, miRNA-mRNA, and lncRNA-mRNA interaction pairs in the 45 vs. 75 comparison group. We obtained 516 lncRNA-mRNA, 1,011 lncRNA-miRNA, and 7,411 miRNA-mRNA relationship pairs. Finally, target genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and it was found that they were mainly enriched in the Wnt signaling pathway and PI3K-Akt signaling pathway related to hair follicle development, indicating that lncRNA may interact with miRNA/mRNA to directly or indirectly regulate the expression of genes related to hair follicle development. Dual-luciferase reporter gene analysis showed that lncRNA MSTRG.1705.1 could bind to Chi-miR-1, while lncRNA MSTRG.11809.1 had no binding site for Chi-miR-433. In conclusion, this study aims to further analyze the molecular regulation mechanism of hair follicle development and to lay a theoretical foundation for revealing the regulation mechanism of cashmere hair follicle growth.
Barriers and Facilitators in Access to Diabetes, Hypertension, and Dyslipidemia Medicines: A Scoping Review
Authors: Castillo-Laborde Carla , Hirmas-Adauy Macarena , Matute Isabel , Jasmen Anita , Urrejola Oscar , Molina Xaviera , Awad Camila , Frey-Moreno Catalina , Pumarino-Lira Sofia , Descalzi-Rojas Fernando , Ruiz Tomás José , Plass Barbara
Published: September 2022

Objective: Identify barriers and facilitators in access to medicines for diabetes, hypertension, and dyslipidemia, considering patient, health provider, and health system perspectives.

Methods: Scoping review based on Joanna Briggs methodology. The search considered PubMed, Cochrane Library, CINAHL, Academic Search Ultimate, Web of Science, SciELO Citation Index, and grey literature. Two researchers conducted screening and eligibility phases. Data were thematically analyzed.

Results: The review included 219 documents. Diabetes was the most studied condition; most of the evidence comes from patients and the United States. Affordability and availability of medicines were the most reported dimension and specific barrier respectively, both cross-cutting concerns. Among high- and middle-income countries, identified barriers were cost of medicines, accompaniment by professionals, long distances to facilities, and cultural aspects; cost of transportation emerges in low-income settings. Facilitators reported were financial accessibility, trained health workers, medicines closer to communities, and patients’ education.

Conclusion: Barriers and facilitators are determined by socioeconomic and cultural conditions, highlighting the role of health systems in regulatory and policy context (assuring financial coverage and free medicines); providers’ role bringing medicines closer; and patients’ health education and disease management.
Framework for Estimating Performance and Associated Uncertainty for Modified Aircraft Configurations
Authors: Denham Casey L. , Patil Mayuresh , Roy Christopher J. , Alexandrov Natalia
Published: September 2022

Flight testing has been the historical standard for determining aircraft airworthiness. However, increases in the cost of flight testing and the accuracy of inexpensive CFD encourage the adoption of certification by analysis to reduce or replace flight testing. A framework is introduced to predict the performance in the special case of a modification to an existing, previously certified aircraft. This framework uses a combination of existing flight tests or high fidelity data of the original aircraft as well as lower fidelity data from CFD or wind tunnel testing of the original and modified configurations to create 6-DOF flight dynamics models. Two methods are presented which generate an updated flight dynamics model and estimate the model form uncertainty for the modified aircraft configuration using knowledge of the original aircraft. This updated dynamics model and uncertainty estimate are then used to conduct non-deterministic simulations with wind turbulence included. The framework is applied to an example aircraft system to demonstrate the ability to predict the performance and associated model from the uncertainty of modified aircraft configurations.
Multi-UAV Unbalanced Targets Coordinated Dynamic Task Allocation in Phases
Authors: Wang Wenfei , Lv Maolong , Ru Le , Lu Bo , Hu Shiguang , Chang Xinlong
Published: September 2022

Unmanned aerial vehicles (UAVs) can be used in swarms to achieve multiple tasks cooperatively. Multi-UAV and multi-target cooperative task assignments are difficult. To solve the problem of unbalanced, phased, cooperative assignment between UAVs and tasks, we establish an unbalanced, phased task assignment model that considers the constraints of task execution, time, and target task execution demand. Based on an improved consensus-based bundle algorithm (CBBA), we propose a two-tier task bidding mechanism. According to dynamic changes in new tasks, we study a dynamic assignment strategy and propose a mechanism based on task continuity adjustment and time windows. Finally, a simulation experiment is used to verify the feasibility and effectiveness of the proposed allocation method in multi-UAV target assignment scenarios. The results show that the dynamic task assignment strategy can efficiently assign random new tasks as they arise.
Exogenous Fibrolytic Enzymes and Length of Storage Affect the Nutritive Value and Fermentation Profile of Maize Silage
Authors: Salvo Pedro , Gritti Viviane , Silva Érica da , Nadeau Elisabet , Daniel João , Spindola Maria , Nussio Luiz
Published: September 2022

The addition of exogenous fibrolytic enzymes (EFEs) and length of storage can affect the quality of maize silage. Therefore, the objective of this study was to evaluate the fermentative profile and the nutritive value of maize silage treated with different doses of EFEs ensiled for 30, 60, or 90 days. The study was designed as completely randomized in a split-plot arrangement of treatments, where four doses of EFEs were assigned to the main plot and three lengths of storage to the sub-plot, with four replicates per treatment. Treatments were: Control, E100 (EFEs at 100 g/ton dry matter (DM)), E150 (EFEs at 150 g/ton DM) and E200 (EFEs at 200 g/ton DM). The EFE treatment did not increase the digestibility of nutrients but increased the acetic acid concentration (1.87 vs. 1.18% DM), while decreasing the content of ethanol (0.02 vs. 0.08% DM), ethyl lactate (7.50 vs. 15.9 mg/DM) and ethyl acetate (5.58 vs. 10.6 mg/DM). Prolonged storage increased DM losses (7.05 vs. 2.32%) and acetic acid content (2.19 vs. 1.03% DM), but decreased ethanol concentration (0.02 vs. 0.09% DM). In conclusion, the addition of EFEs in maize silages did not affect nutrient digestibility and DM losses during fermentation, but it slightly decreased the concentrations of ethanol and esters and increased the acetic acid content. Although statistically significant, such differences may not be relevant biologically, due to the relatively low concentrations of ethanol and esters in all treatments.
Improvement of Wheat Growth Information by Fusing UAV Visible and Thermal Infrared Images
Authors: Yu Jun , Zhou Chengquan , Zhao Jinling
Published: September 2022

To improve and enrich the wheat growth information, visible and thermal infrared (TIR) remote sensing images were simultaneously acquired by an unmanned aerial vehicle (UAV). A novel algorithm was proposed for fusing the visible and TIR images combing the intensity hue saturation (IHS) transform and regional variance matching (RVM). After registering the two images, IHS transform was first conducted to derive the Intensities of two images. Wavelet transform was then applied to the Intensities for obtaining the coefficients of low- and high-frequency sub-bands. The fusion rules of the fused image were developed based on regional correlation of wavelet decomposition coefficients. More specifically, the coefficients of low-frequency sub-bands were calculated by averaging the coefficients of two images. Regional variance was used to generate the coefficients of high-frequency sub-bands using the weighted template of a 3 × 3 pixel window. The inverse wavelet transform was used to create the new Intensity for the fused image using the low- and high-frequency coefficients. Finally, the inverse IHS transform consisting of the new Intensity, the Hue of visible image, and the Saturation of TIR image was adopted to change the IHS space to red–green–blue (RGB) color space. The fusion effects were validated by the visible and TIR images of winter wheat at the jointing stage and the middle and late grain-filling stage. Meanwhile, IHS and RV were also comparatively evaluated for validating our proposed method. The proposed algorithm can fully consider the correlation of wavelet coefficients in local regions. It overcomes the shortcomings (e.g., block phenomenon, color distortion) of traditional image fusion methods to obtain smooth, detailed and high-resolution images.
Prepubertal Use of Long-Term GnRH Agonists in Dogs: Current Knowledge and Recommendations
Authors: Schäfer-Somi Sabine , Kaya Duygu , Aslan Selim
Published: September 2022

The search for an alternative approach of estrus control (induction or suppression) in dogs is an important issue and the use of slow GnRH agonist-releasing implants has been the subject of frequent research in recent years. Studies to date demonstrate that the short- and long-term effects of deslorelin implants applicated at different time points of the prepubertal period are similar to those of adult dogs; however, there are important differences. The age of the prepubertal bitch and the dosage appear to be the main determinants of the response to deslorelin, as well as the individual metabolism of the bitch. Recent studies reported that the deslorelin-mediated long-term delay of puberty does not have negative carry-over effects on subsequent ovarian functionality, serum steroid hormone concentrations, uterine health, and fertility; however, more molecular studies are needed to determine the effects of application time of GnRH agonists on hormone concentrations and peripheral receptor expression. Furthermore, the long-term effects of delay of puberty with deslorelin on joint health, tumor development, the immune system, and social behavior deserve further investigations.
Editorial for Special Issue “Antibacterial Resistance and Novel Strategies to Eradicate Bacterial Biofilms”
Authors: Das Theerthankar
Published: September 2022

Bacterial resistance to antimicrobial agents, including antibiotics, disinfectants, and detergents, is on the rise, with consequences associated with morbidity, mortality, and economic loss in the healthcare sector [...]
Valorization of Agro-Industrial Wastes by Ultrasound-Assisted Extraction as a Source of Proteins, Antioxidants and Cutin: A Cascade Approach
Authors: Mellinas Cristina , Solaberrieta Ignacio , Pelegrín Carlos Javier , Jiménez Alfonso , Garrigós María Carmen
Published: September 2022

The use of agro-industrial wastes to obtain compounds with a high added-value is increasing in the last few years in accordance with the circular economy concept. In this work, a cascade extraction approach was developed based on ultrasound-assisted extraction (UAE) for tomato, watermelon, and apple peel wastes. The protein and antioxidant compounds were obtained during the first extraction step (NaOH 3 wt.%, 98.6 W, 100% amplitude, 6.48 W/cm2, 6 min). The watermelon peels (WP) showed higher proteins and total phenolic contents (857 ± 1 mg BSA/g extract and 107.2 ± 0.2 mg GAE/100 g dm, respectively), whereas the highest antioxidant activity was obtained for apple peels (1559 ± 20 µmol TE/100 g dm, 1767 ± 5 µmol TE/100 g dm, and 902 ± 16 µmol TE/100 g dm for ABTS, FRAP and DPPH assays, respectively). The remaining residue obtained from the first extraction was subsequently extracted to obtain cutin (ethanol 40 wt.%, 58 W, 100% amplitude, 2 W/cm2, 17 min, 1/80 g/mL, pH 2.5). The morphological studies confirmed the great efficiency of UAE in damaging the vegetal cell walls. WP showed a higher non-hydrolysable cutin content (55 wt.% of the initial cutin). A different monomers’ profile was obtained for the cutin composition by GC-MS, with the cutin from tomato and apple peels being rich in polyhydroxy fatty acids whereas the cutin extracted from WP was mainly based on unsaturated fatty acids. All of the cutin samples showed an initial degradation temperature higher than 200 °C, presenting an excellent thermal stability. The strategy followed in this work has proved to be an effective valorization methodology with a high scaling-up potential for applications in the food, pharmaceutical, nutraceutical, cosmetics and biopolymer sectors.