bluuebunny/crossref_metadata_2025 huggingface dataset:
7. The Consideration of Halalan, Thayyiban and Economic Factor of Consuming Chicken Meat in Several Markets of Aceh Besar District
Authors: Rosa Teuku Shaddiq , Ferasyi Teuku Reza , Razali Razali , Budiman Hamdani , Fachrurrazi Fachrurrazi , Nurliana Nurliana
Published: September 2021

This study was aimed to determine the basic consideration of consumers belief on halalan, thayyiban, and to analyze the relationship between socioeconomic status and consumers’ reason when deciding to buy chicken meat in some markets of Aceh Besar District. This study was conducted from March to April 2016 in Lambaro market, Lam Ateuk market, and Ketapang Dua market. A survey approach was used in this research through interview of respondents using structured questionnaire. The respondents were selected randomly from 139 consumers who buy chicken meat in those markets. The criteria of respondent were Muslim consumers, age ≥ 20 years, and buy chicken meat in the study location. The research data were analyzed descriptively. The results of this study showed that most of the costumer's belief that the chicken meat was sold with halal standard. The majority of consumers mentioned that their beliefs was based on the basis of knowing the butcher which is a Muslim (36.69%). In addition, most consumers ( 90%) expressed confidence in the status of thayyiban (safe, healthy and intact) of broiler chicken which sold in all three markets. Age, level of education, and occupation are the factors that affect the mindset of consumers perception in choosing chicken meat that halal thayyiban. On the other hand, the economic status of consumers also can affect the consideration of the halal, thayyiban, when buying chicken at the market. In conclusion, the status of halal and thayyiban of broiler chicken meat is very important for consumers.
11. Study of Beef Consumer Consideration Based On the Concept of Halal and Economic Factors in Butchery of Lambaro, Aceh Besar
Authors: Nuhraini Fuji , Ferasyi Teuku Reza , Rastina Rastina , Ismail Ismail , Nurliana Nurliana , Razali Razali
Published: September 2021

This study was aimed to determine the basic consideration of consumers based on halal and economic factors in beef markets (butchery) at Lambaro, Aceh Besar. A survey approach was used in this research through interview of respondents using structured questionnaire. The respondents were selected randomly from consumers purchased beef. The criteria of respondents were Muslim, aged≥ 20 years and purchased beef in the study location. The data obtained were analyzed descriptively. A number of 90 respondents were interviewed in this survey. The results showed that most of the consumers concerned about the halal aspect. Furthermore, a number of consumers stated that they expect for halal certificate available on the butchery. This is intended to increase consumer’s confidence on halal status of beef. Then, the economic factor also influenced the consumer’s choice for halal beef.
12. The Effect Of Neem Leaves (Azadirachta Indica) Extract To The Blood Profile Of Male Rat (Rattus Norvegicus) Infected With Trypanosoma Evansi Of Krueng Raya Isolat
Authors: Agusty Atika , Fahrimal Yudha , Lubis Triva Murtina , Hambal Muhammad , NA Zuhrawaty , Sayuti Arman
Published: September 2021

This study aimed to determine the ability of neem leaf extract to maintain the normal blood profile of white male rat (Rattus norvegicus) infected with Trypanosoma evansi. A total of 24 male white rats were divided into 6 groups. The K0 group as the negative control received no T. evansi infection and no neem leaf extract. Furthermore, K1  group (positive control) only infected with T. evansi,  K2 and K3 were given neem leaf extract 50mg/kg BW and 100mg/kgBW, while K4 and K5 were given neem leaf extract 400mg/KgBW and  K5 800mg/kgBW. The neem leaf extract was administered for 3 consecutive days after infection established. The blood of the rats was collected to determine the amount of erythrocyte and leukocyte, hemoglobin level and hematocrit value. The data were analyzed using Analysis of Variance (ANOVA) through SPSS for Windows 16.0. The averages (+SD) of erythrocyte of K0, K1, K2, K3, K4, and K5 were 5,64±0,57; 2,31±1; 1,93±0; 0; 2,55±0,33; and 2,56±0,48. The amount of leukocyte were 4,46±1,09; 4,45±1,91; 5,25±0; 0; 8,42±1,66; and 8,14± 5,17. The value of hemoglobin were 12,00±0,47; 10,50±0,57; 4,30±0; 0; 5,60±0,20; and 9,03±0,66. The level of hematocrit 38,00±1,83; 25,00±9,90; 15±0; 0; 18,00±2,65, and 20,75±1,71. The result showed that the administration of neem leaf extract with doses 400mg/kgBW and 800mg/kgBW were difference significantly (P0.05) compare to the positive control of rats, but could not equalize blood profile of uninfected white male rats
8. The Total of Leukocytes Mice (Mus musculus) Exposed To Secondhand Smoke Extract And Given Watermelon (Citrullus vulgaris)
Authors: Mauliza Dewi , Rusli Rusli , Roslizawaty Roslizawaty , Rosmaidar Rosmaidar , Rinidar Rinidar , Masyitha Dian
Published: September 2021

This study aimed to determine the effect of the red watermelon extract can affect the number of leukocytes in mice exposed to cigarette smoke. This study was designed with a completely randomized design using a unidirectional pattern 20 male mice (Mus muscullus) were divided into 4 groups. K1 negative controls were given 0.5 ml of distilled water, K2 positive control by exposure to cigarette smoke and 0.5 ml of distilled water, K3 by exposure to cigarette smoke and red watermelon extract were given a dose of 22 mg / mouse and K4 by exposure to cigarette smoke and were given extracts of red watermelon a dose of 44 mg / mouse. Exposure to cigarette smoke and red watermelon extract conducted for 30 days, making blood on day 31. Blood sampling performed at Plexus Retroorbitalis. Furthermore, counting the number of leukocytes. Data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of watermelon for 30 days showed their real influence (P 0.05) to the number of leukocytes in mice. It is concluded that free radicals contained in cigarette can cause a leukocytosis, an inflammatory, oxidative stress and red watermelon extract can minimize cell damage.
20. The Isolation of Salmonella sp. on quail eggs (cortunxi-cortunix japonica) that failed to hatch in Garot, Darul Imarah Subdistrric, Aceh Besar
Authors: Suryandari Latifa , Erina Erina , Darniati Darniati , Safika Safika , Asmilia Nuzul , Salim M. Nur
Published: September 2021

The study aimed to isolate the Salmonella sp on quail eggs that failed to hatch in Garot village, Darul Imarah Subdistrict, Aceh Besar. A total of thirty quail eggs that failed to hatch were examined in microbiology laboratory of Veterinary Medicine Faculty, Syiah Kuala University. Modification of Carter method was used in this study to isolated samples. The eggs were opened for taking the embryo and then it was swabbed with sterile swabs.  The swab was put into Selenite Cystine Broth (SCB) and incubated at 37o C for 24 hours. The breed was then transferred onto Salmonella Shigella Agar (SSA). The growing Bacteria were observed for their morphology and Gram staining to make sure the bacteria belonged to the negative Gram. The result showed that 10 of the 30 of quail eggs that failed to hatch were positively infected with Salmonella sp. Based on this study, it can be concluded that the salmonella sp.can be isolated from quail eggs which were not hatched in the farm of Garot, Darul Imarah Subdistrict, Aceh Besar
15. Potency of Methanolic Extract of Sernai Stems (Wedelia biflora) as Analgesic on Mice (Mus musculus)
Authors: Rahmatia Annisa Rizka , Rinidar Rinidar , TR T. Armansyah , Rosmaidar Rosmaidar , Harris Abdul , Fahrimal Yudha
Published: September 2021

The purpose of this research was to determine the potency of methanolic extract of sernai stem (Wedelia bilflora) as analgesic to mice (Mus muculus) compared to ibuprofen. This research use split-plot method with 5 treatments and 5 replications. Group P0 as negative control was given aquadest, P1 as positive control was given ibuprofen, while P2, P3, and P4 were given the extract with dosage of 40, 50, and 60 mg/kg bw, respectively. The pain analysis of mice was conducted using hotplate method and the observation was carried out at 0 minute, 30 minutes, 60 minutes, 90 minutes, and 120 minutes. The data was analyzed using analysis of varians (Anova) followed by Duncan’s test. The results of this research showed  that P1, P2, P3 and P4 had significant difference (P0,05) from P0. P1 did not have significant  difference (P0,05) from P2 but it was different from P3 and P4 (P0,05). From this result we can conclude that methanolic extract of sernai stem was potential as analgesic, dosage of 40 mg/kg bw was equal to ibuprofen, while 50 mg/kg bw and 60 mg/kg bw showed better effect than ibuprofen.
21. Physical Quality and Organoleptik of Beef Added with Curry Leaf (Murraya koenigii) Infuse
Authors: Murliana Baida , Rastina Rastina , Ismail Ismail , Nurliana Nurliana , TR T. Armansyah , Aliza Dwinna
Published: September 2021

The aims of this research were to compare the physical charateristic of meat with or without the addition of curry leaf and to find out the organoleptic of meat added with curry leaf. This research used complex randomized design method. 500 grams beef sample were cut into square-shape at 2x2x2 cm  and divide into 3 groups and  then added a curry leaf infuse with different concentration of 0%, 25%, and 50%. Then, the meat was boiled at 70-80oC for 45 minutes. The parameters observed were pH, water holding capacity, cooking loss, and organoleptic (colour, taste, and aroma). The result showed that the use of curry leaf infuse in various concentration did not have significant effect on pH, water holding capacity, and cooking lost, but significantly effect (P0,05) on organoleptic value. Fresh meat that was added the curry leaf infuse 50% was well-liked for the colour, taste, and aroma  compare to the meat without the addition of curry leaf infuse.
19. Histological of Thymus in Tegal Duck (Anas javanicus) at Different Ages
Authors: Budiman Hamdani , Septian Anugrah , Hamny Hamny , Zainuddin Zainuddin , Iskandar Cut Dahlia , Sabri Mustafa , Hambal Muhammad , Harris Abdul
Published: September 2021

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages. The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks.  The distribution of Hassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer.
20. Ectoparasites Identification of Stingrays Fish (Dasyatis Sp.) At Peunayong Fish Market, Banda Aceh
Authors: Muttaqien Muttaqien , Khaira Arini Ulfa , Winaruddin Winaruddin , Eliawardani Eliawardani , Hambal Muhammad , Azhar Al
Published: September 2021

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages.The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks. The distribution ofHassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer
2. The Effect Of Sipatah-patah (Cissus quadrangularis Salisb) Extract On The Femur Bone Density of White Rat (Rattus norvegicus) with Model Ovariectomy
Authors: Sabri Mustafa , Ayumi Debby Novita , Jalaluddin Muhammad , Hamny Hamny , Iskandar Cut Dahlia , Herrialfian Herrialfian
Published: September 2021

This research aimed to determine the effect of giving Sipatah-patah extract toward the histopathological and femur bone growth of white rat that was ovariectomized. The experimental animals that were used were 12 white rats divided into 4 treatment group with 3 repetitions. K0 was the ovariectomized rat without giving Sipatah-patah extract (ESP); K1, K2 and K3 were ovariectomized rats which were given Sipatah-patah extract with multilevel doses of 500 mg/kg BW, 700 mg/kg BW and 900 mg/kg BW for 30 days. On the 31st day, rats were euthanized using chloroform and os femur that was taken to being made into histological preparation.  There was the decrease in the bones density of the K0 group which is characterized by thinning of trabecular structure, there were lots of osteoclast cells on the edge of the trabecular and lower density of active osteoblasts and passive osteoblasts. The rat of group K1 and K2 showed an improvement on the trabecular structure and lower osteoclast than group K0. The rat of group K3 had a visible improvement of the most congested trabecular structures, cohesive with the most density cell of active osteoblasts than the other groups. The result of this research concluded that the giving of Sipatah-patah extract doses 900 mg/kg BW showed a higher density of trabecular and active osteoblasts than the control group, K1 and K2 on the white rat bones that were ovariectomized.
9. The Isolation of Steptococcus sp on horse’s dental caries in Aceh Tengah Regency
Authors: Ahmad Sagasias , Erina Erina , Daud AK M , Darmawi Darmawi , Fakhrurrazi Fakhrurrazi , Isa M.
Published: September 2021

The purpose of this study was to detect the existence of the Streptococcus sp bacteria in the horses’ dental caries in Aceh Tengah Regency. The caries samples used in this study were taken from 8 horses in Aceh Tengah Regency. The samples were collected using swab on the teeth and then put into Nutrient Broth (NB) media and further were cultured on selective media TSY20B. The bacteria identification was conducted by observing the bacteria’s morphology, Gram staining and biochemical test. The eight samples of the horses’ dental caries showed that Streptococcus sp bacteria could be isolated from all of the collected samples. From this research it could be concluded that Streptococcus sp bacteria was found in the horses’ dental caries and it was one of the causes of the dental caries.
17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate
Authors: Dewi Maryulia , Darmawi Darmawi , Helmi T. Zahrial , Erina Erina , Gani Basri , Eliawardani Eliawardani , Azhar Azhar
Published: September 2021

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.
34. Anatomical Pathology And Histopathological Changes Of Ascaridia Galli In Layer Chicken
Authors: Hambal Muhammad , Efriyendi Rizki , Vanda Henni , Rusli Rusli
Published: September 2021

This study aimed to determine the anatomical pathology and histopathological, and their relationship of the ascaridiosis caused by Ascaridia galli worms infection that have been found in layer chickens after necropsy, thus facilitating the diagnosis, treatment, and prevention of ascaridiosis. This study used 1 dead chicken from the Lambaro traditional market, Ingin Jaya District, Aceh Besar Regency. The sample was taken to the Pathology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University for dissected and observed in anatomical pathology changes, then histopathological preparations were made to observe histopathological changes. Data were obtained then analyzed descriptively and presented in the form of images. The results of anatomical pathology examination in chickens infected with ascaridiosis were found the Ascaridia galli worms infestation in the intestinal lumen with moderate infestation rates and focal area of hemorrhage in the intestinal mucosa. Then, histopathological examination were found desquamation of villous epithelium, hemorrhage in the intestinal mucosa, inflammatory cell infiltration, and proliferation of cryptic cells caused by A. galli worms infection. Based on the results of the examination concluded that there is an association between anatomical and histopathological pathology findings, namely hemorrhage in the intestine.
38. Density Figure Of Aedes Spp Larvae In Jeulingke Village Syiah Kuala Subdistrict Banda Aceh
Authors: Athailah Farida , Hanafiah Muhammad , Prapanca Eka Ichsan , Riandi Lian Varis , Eliawardani Eliawardani , Muttaqien Muttaqien
Published: September 2021

This study was aimed to determine the level of Density Figure of Aedes spp mosquito larvae with parameter House Index (HI) Container Index (CI), and Breteau Index (BI) at Jeulingke Village, Syiah Kuala Subdistrict, Banda Aceh. This research was conducted by using Single Larva Method. Single Larvae Method is a method used to take a single larva by using a plastic scoop on each container that is found to have a larva and visually observed by using a flashlight. The sample used consisted of 120 houses taken randomly from 6 hamlets. The larval data were obtained from various types of containers tabulated into tabular form, then the larvae index was calculated to determine the density of the larvae based on the Density figure criteria. The results showed that from 120 houses found 702 containers in Jeulingke Village with 20 positive larval houses and 20 containers that positively contained larvae such as 19 bath containers and used tires by 1 container so that the index of larvae obtained with House Index is 17% , Container Index of 3%, Breteau Index 20, so as to obtain Density Figure value in the range of numbers 2-4. The results show that the Density Figure in Jeulingke Village is in the medium category.
39. Efficacy of Jatropha cream (Jatropha curcas L.) on maturation phase of cutaneus healing process in Mice (Mus Musculus)
Authors: Harris Abdul , Amran Cut Mentari Fatihah , Salim M. Nur , Balqis Ummu , Armansyah T. , Karmil T. Fadrial , Riady Ginta
Published: September 2021

This study aims to determine the efficacy of jatropha curcas L. cream in the maturation phase of healing of mice leather injuries. This study used nine rats with 3 treatments, ie treatment group I smeared cream base (P1), treatment group II smeared 0.1% sulfadiazine cream (P2) and treatment group III smeared jatropha curcas 10% (P3). The result of ANAVA test of treatment I, treatment II and treatment III had significant effect (P 0,05) on fibroblast cells but each treatment group on collagen density level showed significant effect (P 0.01). Duncan test results on fibroblasts, P3 was significantly different (P 0.05) with P2 and P3, whereas the results on P3 collagen density were significantly different (P 0.01) with P2 and P1. The conclusion that 10% jatropha gum cream for 10 days can decrease the number of fibroblast cells and increase the amount of collagen thus accelerate the process of maturation phase of healing of mice leather injuries.
40. Knowledge and Perception of Cervical Cancer
Authors: Halifah Elka , Mutiara Erna , Masniari Lubis Ria
Published: September 2021

Cervical cancer is a type of cancer which ranks the second of whole incident of cancer in women. Cervical cancer can metastatic to other organs and can cause death. The objective of the research was to knowlage and perception about cervical cancer. The type of research was a survey with descriptive design. The population of women in Banda Aceh and 60 of them were used as the samples using accidental sampling technique. The data were collected by interview and analyzed by using univariat.  There were influence of poor knowledge 63,3%, and 75,0% negative perception about cervical cancer. It is recommended that the local Government, Health Office and Indonesian Cancer Foundation in Nanggroe Aceh Darussalam Province provide convenience in service  counseling in order to increase knowledge. The movement of early detection  program (papsmear) need to be improved so that women sexually active are willing to examine themselves to the health service so that there will be no lateness in having treatment.
31. Histological of Tractus Digestivus of Domestical Catfish (Clarias batracus)
Authors: Irfandi Aliman , Iskandar Cut Dahlia , Zainuddin Zainuddin , Masyitha Dian , Fitriani Fitriani , Hamny Hamny , Panjaitan Budianto
Published: September 2021

Domestical catfish is grouped into omnivora, although the fish is tended into carnivora.  The aim of the present study was to investigate histology of alimentary tract of domestical catfish (Clarias batrachus). In order to determine alimentary tract of domestical catfish, oesophagus, gastric and intestines is investigated by Haematoxilin Eosin. Histology exploration was performed each layer of alimentary tract’s wall consist of lamina epithelia, propria, and muscularis mucosa. Collagen connective tissue was developed tunica submucosa. The muscle of tunica muscularis was performed circular and elongated. Dense connective tissue and blood vessels was located in the tunica serosa. Histology exploration revealed has stratified squamous epithelium, mucosit cells, eosinophilic club cells on the lamina epithelia, and taste buds on the lamina propria in oesophagus. Gastric was establised simple prismatic cells, and glands of gastric on the lamina propria. Furthermore intestine was showed villi and goblet cells. There were not showed Brunner and Liberkhun gland. In conclusion, histology of alimentary tract was revealed from tunica mucosa, submucosa, and serosa.
25. The effect of Fingerroot Rhizome (Boesenbergia pandurata) Extract on the Growth of Staphylococcus aureus in Vitro
Authors: Keliat Silvia P.N , Darniati Darniati , Harris Abdul , Erina Erina , Rinidar Rinidar , Fahkrurrazi Fahkrurrazi
Published: September 2021

This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.
3. Flies Larva On White Rat Carcass (Rattus norvegicus Berkenhout, 1769) With Various Treatment Outdoor
Authors: Indra Putra Ichsan Luqmana , Yahya Sadda Salisa
Published: September 2021

Insect activity including its life cycle can determine the estimated time of death or Post Mortem Interval (PMI). One type of insect that plays an important role in determining the estimated time of death is flies. This study aims to determine the types of flies that come to the carcass and to determine the types of flies that dominate and less dominate from each treatment onwhite rat (carcassesR. norvegicusmale) outdoors. This study consisted of 3 treatments with 3 repetitions each. The treatments included A (neck bone dislocation), B (burned), and C (poisoned). Carcass observations were carried out for 10 days and larvae collection was carried out every 2 days starting from the third day of observation. The parameters observed included the number and morphological characteristics of instar 3 fly larvae. The types of fly larvae found in all treatments were Lucilia illustris (5.42%), Sarcophaga sp (12.80%), Sarcophaga argyrostoma (30.62%), and Sarcophaga variegata (51.16%). The dominant fly larvae of the three treatments was S. variegata and the less dominant fly larvae was L. illustris.
Identify Chemical Compounds of Wedelia biflora and Test Their Bioactivity as Antiplasmodium Berghei
Authors: Isa M.
Published: September 2021

The aims of the research is to identify chemical compounds of leaf Sernai (Wedelia biflora) and test their bioactivity as antiplasmodium against of Plasmodium berghei. Extraction of the compounds from the Wedila biflora leaf was done with methanol. To observe the bioactivepotency of the Wedelia biflora extract antiplasmodium test against with The 4-day test method was done. The Phytochemical test results using theLieberman-Burchard reagent positive for triterpenoids compounds and analysis by GC-MS chromatogram identified 45 compounds with anabundance peak maximum of 27.92% which is identified in the data base as kaur-16-en-18-oic acid. Wedelia biflora leaf extract potentiallyactive as antiplasmodium with IC values = 39.952 mg / kg BW.____________________________________________________________________________________________________________________Key words: antiplasmodium, GC-MS, Plasmodium berghei, Wedelia bifolra 50
The Potential of Bandotan Leaves (Ageratum Conyzoides L.) Extract for Natural Curing of Eggs
Authors: Novita Andi
Published: September 2021

This study aims to determine the effect of eggs immersed in extracts of bandotan leaves on the organoleptic quality, haugh unit, and yolk index. The samples used were 105 eggs, weighing of 51.33 ± 55.9 grams collected from the farm in Jantho. By using a completely randomized design with factorialpattern the samples were divided into five treatment groups. P0 (control) was not given any treatment , P1 was immersed in 10 % bandotan leaf extract for12 hours, P2 was immersed in 10% bandotan leaf extract for 24 hours , P3 was immersed in 20% bandotan leaf extract for 12 hours and P4 wasimmersed in 20% bandotan leaf extract for 24 hours. The observation interval was done on week 0, 1, 2, 3, and 4. The organoloeptic observation of alleggs showed that either eggs on P1and P2 treatment showed no significant changes until week 4. The HU value of P0, P1, and P3 were 68.16 (grade A),70.34 (grade A), and 75.89 (grade AA), respectively. Yolk index value of P0, P1,and P2 were 0.65, 0.71, and 0.74 , respectively. In conclusion, the higherthe concentration of bandotan leaf extract given the longest the storage time of eggs based on the maintain of HU and YI value.____________________________________________________________________________________________________________________Key words: bandotan leaf, haugh unit, organoleptic, egg, yolk index
The Effect of pineaple juice (Ananas comosus) on Blood Cholesterol Level of Rabbit (Oryctolagus cuniculus) hipercholesterolemia
Authors: A. Zuhrawati N.
Published: September 2021

This study aims to know the effect of pineapple juice on blood cholesterol level of male hypercholesterolemic rabbits. A total of 12 local male rabbits aged 1-2 years with body weight ranging from 0.9-1.6 kg were used as sampel. Sample were divided randomly into 4 treatmentgroups, each group consisting of 3 replications. All rabbits were initially examined for the total cholesterol levels, before fed with a diet to makehypercholesterolemic. The rabbits were then fed with a mixture of 90% sugar solution as much as 6 gr/kg body weight and egg yolks as much as3 gr/kg body weight for 7 consecutive days using a stomach sonde, followed with re-examination of total cholesterol. Samples were thenadministered with pineapple juice on KI (10 g /kg BW), KII (20 g/kg BW), and KIII (30 g/kg), while the K0 as controls were not given pineapplejuice. After 10 days of administration of pineapple juice, rabbit blood was collected through the auricular vein for total cholesterol examinationusing the Chod-PAP. Data were analyzed by ANOVA completely randomized design (CRD) one way, and the difference between the treatmentgroup was examined using Duncan's multiple test. Results showed that the mean (±SD) of total blood cholesterol levels of rabbits (mg/dl) groupsK0, KI, KII, and KIII after the administration of pineapple juice in a row was 176.60 ± 57.10; 66.60±12.50; 63±17.70; and 57.60±24.80.Statistical analysis showed that treatment affect significantly (P0.05) to decrease total blood cholesterol levels of rabbits. In conclusion, theadministration of pineapple juice (Ananas comosus) for ten consecutive days can decrease blood total cholesterol of hypercholesterolemiarabbits.____________________________________________________________________________________________________________________Key words: diet cholesterol, pineapple juice, total cholesterol levels, rabbit
Effect of Temperature Increase on Hemoglobin Concentration and Hematocrit Value of Tilapia Fish (Oreochromis niloticus)
Authors: A. Zuhrawati N.
Published: September 2021

This research aims to determine the effect of temperature increase on hemoglobin consentration and hematocrit value of tilapia fish. This research was conducted at Faculty of Veterinary Medicine Syiah Kuala University Banda Aceh. Samples used were blood from tilapia fish thatderived from 12 tilapia fish with weight range of 40-50 g. The research design used was a completely randomized design (CRD) unidirectionalpattern with 3 treatments and 4 replications. P1 as a control group was the treatment with aquarium temperature of 29±1C, P2 was thetreatment with aquarium temperature of 32±1o C, and P3 was the treatment with aquarium temperature of 35±1o C. All aquarium size in thisstudy was 80 cm x 60 cm x 40 cm with the height of water 30 cm. The treatment was carried out for 15 days and on day 16 blood samples werecollected. Blood was taken through the caudalis vein. Data were analyzed by analysis of variance (ANOVA) one way, followed by Tukey test.Mean (± SD) of tilpia fish hemoglobin levels (g/dl) on P1, P2, and P3 were 4.50±0.49, 5.08±0.79, and 6.08±0.94, respectively. Mean (± SD) oftilapia fish hematocrit value (%) in P1, P2, and P3 consecutively were 26.50±8.10, 33.25±11.78, and 40.25±9.04. Statistical analysis showed thatan increase in temperature showed no effect (P 0.05) to the hemoglobin levels of tilapia fish and highly significant to the hematocrit value (P0.01). It can be concluded that the increase of water temperature on 32±1° C and 35±1° C in can increase the hemoglobin levels andhematocrit value of tilapia fish.____________________________________________________________________________________________________________________Key words: tilaphia fish, hemoglobin levels, hematocri value, temperature o
Proximate Analysis of Protein and Relative Growth of Tilapia Fish Exposed to Heat Stress and Fed with the Combination of Willow Leaf and Chromium
Authors: Widodo Ari , Isa Muhammad , TR Teuku Armansyah
Published: September 2021

This study was aimed to determine the effect of relative growth rate and proximate levels of protein in tilapia fish exposed to heat stress and supplemented with the combination of willow leaf powder and chromium. A total of 30 tilapia fish with 1.3-1.5 cm long were divided into 6 treatment groups with 5 repetitions: fed with commercial feed at temperature 29±1° C (P1S1), fed with commercial feed combined with willow leaf at temperature 29±1° C (P2S1), fed with commercial feed combined with willow leaf and chromium at temperature 29±1° C (P3S1), fed with commercial feed at temperature 35±1° C (P1S2), fed with commercial feed combined with willow leaf at temperature 35±1° C (P2S2), fed with commercial feed combined with willow leaf and chromium at temperature 35±1° C (P3S2). The treatments were carried out for 15 days. Then, relative growth rate and proximate levels of proteins were calculated using Kjedahl methods. The results showed that there was an increase of the mean of relative growth rate and proximate levels of protein. Based on statistical test using factorial completely randomized design it was showed that there was a significant effect (P0.01) on the interaction of feeding and temperature with the relative growth rate, but did not affect the relative growth rate of tilapia fish. The proximate level of tilapia fish protein was affected very significantly (P0.01) by the food and the interaction between food and temperature as well, while the temperature treatment gave no significant effect on the proximate level of tilapia fish protein. In conclusion, the commercial feed combined with willow leaf and chromium can increase the relative growth rate and the proximate level of protein of tilapia fish exposed to heat stress.Key words: relative growth rate, proximate protein, willow leaf, chromium, tilapia fish
Parasitemia of Male White Mice (Mus musculus L.) DDY Strain Infected with Trypanosoma evansi Pidie and Pemalang Isolate
Authors: Sari Febiola Rama , Fahrimal Yudha , Balqis Ummu , Subekti Didik T , Wardana Aprilia
Published: September 2021

This study aimed to find out the parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Bbalitvet Bogor. A total of 12 mice were divided into 3 treatment groups. Group 1 (K1) without any treatment, group 2 (K2) was inoculated with 104 T. evansi Pidie isolate, and group 3 (K3) was inoculated with 104 T. evansi Pemalang isolate. Parasitemia examination was carried out every two days and the level of parasitemia was observed as well. Parasitemia examination was conducted until all mice died. Parasitemia of mice infected with Pidie isolate was characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. The parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal was dead. In conclusion, there was the difference in parasitemia level between Pidie and Pemalang isolates. Key words: Trypanosoma evansi, parasitemia, Pidie isolate, Pemalang isolate
Effect of Bandotan (Ageratum conyzoidez L) Leaf Extract on Total Blood Cholesterol Levels of Broiler
Authors: Supriyadi Egi , Roslizawaty Roslizawaty , Zuhrawati Zuhrawati
Published: September 2021

This study aimed to determine the effect of bandotan (Ageratum conyzoides L) leaf extract on total blood cholesterol level of broiler. This study used 15 broiler aged of 25 days. This study was an experimental study using completely randomized design (CRD) unidirectional pattern with 3 treatment groups and 5replications each. Group 0 (P0) was not given bandotan leaf extract, group 1 (P1) was given 3 ml bandotan leaf extract, and group 2 (P 2) was given 5 ml bandotan leaf extract. The administration of Bandotan leaf extract was carried out once a day for 5 consecutive days. On day 6 after treatment, the blood was collected then examined for total blood cholesterol level by precipitation method. Data was analyzed using analysis of variance. The result showed that the average of total cholesterol level on group P0, P1, and P2 was 134.20+10.50, 125.15+8.20, and 113.34+7.50 mg/dl, respectively. In conclusion, bandotan leaf extract decrease blood cholesterol levels of broiler.Key words: broiler, bandotan leaf extract, total cholesterol level
The Effect of Katuk (Sauropus androgyrus) Leaves Administration to the Size of scrotal Circumference of Male lokal Kacang Goats
Authors: Yusman Rahmat , Ferasyi Teuku Reza , Muttaqien Muttaqien
Published: September 2021

The purposed of this study was to test the administration effects of katuk leaves to the size of scrotal circumference of male local kacang goat. This study used 15 male local kacang goats with the age of +1,5 year old. These animals were divided into three groups,5 animals each and acclimatized for 2 weeks. The control group (P0) was given distilated water, group P1 was administered with katuk leaves powder at the dose of 7.44 g/head/day and group P2 was administered with katuk leaves powder at the dose of 14.88 g/head/day. All treatments were given per oral twice a day (at 07.30 AM and 16.30 PM) for 35 days, consecutively. All animals were given various pastures and grasses as well as water ad libitum every day. Pre and post treatment, all animals from each group were measured for the scrotal circumference using vernier caliper. Data were analyzed using ANOVA. The result showed that the average of scrotal circumference on P0, P1, and P2 were 1.99+1.00, 2.75+0.01, and 2.76+0.01 cm. There was significant different (P0.01) on the size of scrotal circumference between all groups. In conclusion, the administration of Sauropus androgyrus leaf powder increases the size of scrotal circumference of male local goat.Key words: scrotal circumference, willow leaf, male local goat
Masculinization of Tilapia (Oreochromis niloticus) Using Methyl Testosterone Natural Hormone
Authors: R Rosmaidar , W Winaruddin , Herlina Mimi
Published: September 2021

This study aimed to determine the effect of methyl testosterone natural hormoneon the rate of masculinization of tilapia (Oreochromis niloticus). The study used 270 tilapia larvae with the age of 7 days as experimental animals. Tilapia larvae were divided into 3 treatments; eachtreatment consisted of 30 larvae fish. This study was an experimental study using completely randomized design with 3 treatments and 3replications. Treatment I as control group was fed with pellets ad libitum, treatment II was given feed containing methyl testosterone naturalhormone, and treatment group III was given hormone treatment by soaking the fish larvae with methyl testosterone natural hormone for 20hours. Larvae were raised in accordance with applicable procedures. Observation was conducted when the larvae fish were at 60 days old andthe sex of fish can be observed. Parameter examined was the percentage of the male sex. The average (± SD) percentage of male tilapia in groupPI, PII, and PIII were 36.39±3.97, 66.72±6.13, and 82.37±6.87%, respectively. The results showed that the percentage of male sex of tilapia fedwith natural hormone of methyl testosterone through the diet and dipping was significantly different (P0.05) from control fish. In conclusion,the administration of natural hormone of methyl testosterone increased the percentage of the male sex of tilapia).____________________________________________________________________________________________________________________Key words: tilapia larvae, natural hormone methyl testosterone, the male sex percentage
The Potency of Sernai (Wedelia biflora) Leaf Water Extract as Antipyretic Compared to Amino Fenol Derivate and Salicylic Acid on Mice (Mus Musculus)
Authors: Rinidar Rinidar , Armansyah Teuku , Putri Tesha Aprilya
Published: September 2021

The study was conducted to screen the antipyretic activity of aqueous of extract of the leaves of sernai (Wedelia biflora) with split-plot design. Eighteen healthy mice weighing between (20-25 g), aged 2-3 months were divided into six groups of three animals each: negative control group were given distilled water (P0), P1, and P2 as a positive control respectively given salicylic acid 42.25 mg/kg body weight, the amino phenol 25 mg/kg body weight, group P3, P4, and P5 given of sernai leaf aqueous extract at a concentration of 50, 75, and 100% respectively. The initial rectal temperature of each animal was recorded by digital thermometer. The pyrexia was induced by injecting a solution of 12.5% peptone. The difference in temperature was observed every 30 minutes for 3 hours and respective time interval was found out by statistical method. The results showed that the water extract of leaves sernai with various concentrations significantly different (P0.01) with an observation time. Average temperatures in the P5 mice was significantly different (P0.05) with P0. P3 and P4 is not significantly different from P0, but P3 and P4 significantly different (P0.05) with P2 and not significantly different from P1. P5 was not significantly different from the P2 and P1 significantly different. It was concluded that the aqueous extract of leaves sernai potential as an antipyretic in mice and at concentrations of 50 and 75% effective in reducing fever in mice tend equivalent to the amino phenol, while the concentration of 100% with salicylic acid.Key words: amino fenol, antipyretic, salicylic acid, sernai
Analysis of the Role of Keeper as a Risk Factor for Infection of Gastrointestinal Nematodes in Sumatran Orangutan in the Re-introduction Station of Jantho
Authors: Murtala Ali , Ferasyi T. Reza , Rahmi Erdiansyah
Published: September 2021

This study was aimed to identify the role of keeper as a risk factor for the infection of gastrointestinal nematodes on orangutans in Jantho Re-introduction Station. This study was conducted using a census method. A number of 8 orangutan’s keepers were involved as respondent. The data was collected by interview using a structural questionnaire. In addition, feces of 6 orangutans caged in this location were taken as sample. The feces sample was then examined for gastrointestinal nematodes using native and centrifugation methods in Parasitology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University. The data obtained from this study were analyzed descriptively. The results of study by questionnaire showed that a possible potential risk factor for the infection of gastrointestinal nematode to orangutans in Jantho Re-introduction Station is the keeper avoids using gloves when supplying food to the animals. Then, the result of laboratory examination showed that it was confirmed that the feces of orangutans infected with gastrointestinal nematodes, which the prevalence of 16.5% for infection by Strongiloides sp. In conclusion, orangutans caged in Jantho Re-introduction Station are in a low infection of gastrointestinal nematodes of Strongiloides sp., and the keeper shows a good behavior to prevent gastrointestinal nematodes infection to orangutan.Key words: keeper, Sumateran orangutan, Pongo abelii, Strongyloides sp.
Asistencia a clase virtual en directo como factor de éxito académico en estudios de posgrado a distancia
Authors: Hernández-Ramos Juan Pablo , Torrijos-Fincias Patricia , García-Barrera-Trujillo María José
Published: September 2021

La irrupción de la tecnología en los diferentes campos de la sociedad no solo genera nuevas necesidades, sino que posibilita nuevas herramientas formativas, como, por ejemplo, la educación virtual, la cual supera las barreras de tiempo y espacio. En la actualidad, el número de personas que deciden cumplimentar sus estudios principales con formación de posgrado a distancia ha aumentado considerablemente, de modo que es necesario, de cara a la mejora de estos entornos formativos, profundizar en determinados aspectos. Así, el principal objetivo del presente trabajo fue analizar si la asistencia a clases virtuales en directo es uno de los factores que influyen de manera positiva en el rendimiento. Para esto se empleó una metodología no experimental, con una muestra no probabilística de 458 estudiantes del máster universitario en Educación Especial de la Universidad Internacional de la Rioja. Tras el empleo de la prueba U de Mann-Whitney como técnica de contraste de hipótesis, se concluye que los alumnos que asisten a las clases virtuales en directo obtienen calificaciones más altas en el examen en relación con los que no asisten. A nivel pedagógico, los resultados detectados constatan cómo la posibilidad o no del estudiante para asistir de manera síncrona a la clase es un factor relevante a considerar por parte del docente a la hora de planificar la docencia de las asignaturas en formato virtual.
DDR Based PWM Technique for Fixed Frequency Three Phase to Variable Frequency K-Phase Power Conversion Using QZSDMC
Authors: Soundarapandiyan Manivannan , N Saravanakumar , N Vijeyakumar K
Published: September 2021

The authors have requested that this preprint be removed from Research Square.
Constructing mRNA, miRNA, circRNA and lncRNA Regulatory Network by Analysis of Microarray data in Breast Cancer
Authors: Hassani Bita , Mollanoori Hasan , Pouresmaeili Farkhondeh , Asgari Yazdan , Ghafouri-Fard Soudeh
Published: September 2021

Background. Luminal tumors are the utmost frequent subtype of breast cancer (BC). Despite luminal BC has relatively good prognosis, in a subset of patients, disease relapse occursto endocrine therapy ;hence, there is a critical need to identify new strategies to promote the early detection and more effective therapies. Noncoding RNAs including microRNAs, long noncoding RNAs, and circular RNAs can interact with and modulate each other via diverse molecular mechanisms and make a complicated regulatory network.ncRNAs participate in diverse biological processes and disorders such as breast tumors. Therefore, understanding their regulatory mechanisms allow to develop new field of research and therapeutic options for BC patients.Methods. In this study, BC-specific RNA expression profiles including mRNAs, miRNAs, lncRNAs, and circRNAs were retrievedfrom Gene Expression Omnibus microarray datasets, and differentially expressed(DE) items were obtained. Disease ontology, functional and pathway enrichment analyses were executed. The protein-protein interaction network was constructed, and hub mRNAs were extracted.The prognostic value of hub mRNAs in patients of BC were performed. Subsequently, a ceRNA network was established.Results. In total, 691 DE genes, 122 DE lncRNAs, 60 DE miRNAs, and 38 DE circRNAs in breast tumor samples were compared with normal samples. Subsequently, 12 hub-genesincluding FOXO3, RHOA, EZH2, KIT, HSP90B1, NCOA3, RAC1, IGF1, CAV1, CXCR4, CCNB1, and ITGB1 were screened from the network. Kaplan-Meier Plotter results revealed that FOXO3 and RHOA were a suitable prognostic marker for patients with breast cancer. Finally, we determined possible ncRNAs (circ0007535, circ0002727, circ0005240, circ0014130, circ0044927, circ0007001, circ0089153,NORAD, MALAT1, TUG1, ZFAS1, OPI5-AS1, miR183,miR182, miR101, miR200c, miR200b, miR149, miR342, and miR1207) which could crosstalk with each other to regulateFOXO3 and RHOA through different regulatory patterns. Conclusion. These data might improve our perception of the breast tumorigenesis and could develop new field of research and therapeutic options for BC patients.