bluuebunny/crossref_metadata_2025 huggingface dataset:
A Convex Dynamic Approach for Globally Optimal Profit in Supply Chains
Authors: Azizian Mojtaba , Sepehri Mohammad Mehdi , Rastegar Mohammad Ali
Published: February 2022

Supply chain finance aims to coordinate multiple stakeholders to maximize the flow of cash and internal and external funding along the supply chain, as shown in prior research. From a regulatory standpoint, the goal of this paper is to maximize the profitability of an entire supply chain. As a result, a constrained finite time Linear Quadratic Regulation (LQR) approach is provided for determining an entity’s optimal profit state in a supply chain. The framework is represented by discrete-time linear dynamical equations for each entity in the supply chain network, taking state and input variables into account. The problem is formulated in terms of a convex quadratic programming optimization for which several numerically efficient algorithms are readily available. In order to validate the approach, it was tested on two topologies. The first topology is a fully connected supply chain with six nodes; the second is a simple topology based on the Iranian pharmaceutical supply chain. The results indicate that the proposed approach successfully planned production and financing decisions within the simulated supply chain and obtained globally optimal profit for all supply chain stakeholders.
Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium
Authors: Terasawa Masahiro , Hiramoto Keiichi , Uchida Ryota , Suzuki Koji
Published: February 2022

We previously reported that rhamnan sulfate (RS) purified from Monostroma nitidum significantly suppressed lipopolysaccharide (LPS)-induced inflammation in cultured human vascular endothelial cells. Here, we analyzed the effect of orally administered RS on LPS-induced damage to mouse organs and vascular endothelium. RS (1 mg) was orally administered daily to BALB/c mice, 50 μg of LPS was intraperitoneally administered on day 8, and Evans blue was injected into the tail vein 6 h later. After 30 min, LPS-treated mice showed pulmonary Evans blue leakage and elevated plasma levels of liver damage markers, whereas this reaction was suppressed in LPS + RS-treated mice. Immunohistochemical and Western blot analysis of mouse organs 24 h after LPS treatment showed significant neutrophil infiltration into the lung, liver, and jejunum tissues of LPS-treated mice and high expression levels of inflammation-related factors in these tissues. Expression levels of these factors were significantly suppressed in LPS + RS-treated mice. Analysis of lung glycocalyx showed a significant reduction in glycocalyx in LPS-treated mice but not in LPS + RS-treated mice. Levels of syndecan-4, one of the glycocalyx components, decreased in LPS-treated mice and increased in LPS + RS-treated mice. The current results suggest that orally administered RS protects organs and vascular endothelium from LPS-induced inflammation and maintains blood circulation.
Clinical Assessment of the Hyperbaric Oxygen Therapy Efficacy in Mild to Moderate Periodontal Affections: A Simple Randomised Trial
Authors: Burcea Alexandru , Mihai Laurenta Lelia , Bechir Anamaria , Suciu Mircea , Bechir Edwin Sever
Published: February 2022

Background and Objectives: Gum disease represents the condition due to the dental plaque and dental calculus deposition on the surfaces of the teeth, followed by ulterior destruction of the periodontal tissues through the host reaction to the pathogenic microorganisms. The aim of study was to present aspects regarding the efficacy of hyperbaric oxygen therapy (HBOT) as an adjuvant therapy for the treatment of periodontal disease, started from the already certified benefits of HBOT in the general medicine specialties. Materials and Methods: The participant patients in this study (71) required and benefited from specific periodontal disease treatments. All patients included in the trial benefited from the conventional therapy of full-mouth scaling and root planing (SRP) within 24 h. HBOT was performed on the patients of the first group (31), in 20 sessions, of one hour. The patients of the control group (40) did not benefit from HBO therapy. Results: At the end of study, the included patients in HBOT group presented significantly better values of oral health index (OHI-S), sulcus bleeding index (SBI), dental mobility (DM), and periodontal pocket depth (PD) than the patients of the control group. Conclusions: HBOT had beneficial effects on the oral and general health of all patients, because in addition to the positive results in periodontal therapy, some individual symptoms of the patients diminished or disappeared upon completion of this adjuvant therapy.
Effect of Prolonged Thermal Exposure on Low-Cycle Bending Fatigue Resistance of Low-Carbon Steel
Authors: Nikulin Sergey A. , Rogachev Stanislav O. , Belov Vladislav A. , Zadorozhnyy Mikhail Y. , Shplis Nikolay V. , Skripalenko Mikhail M.
Published: February 2022

Using a dynamic mechanical analyzer, the comparative studies of a low-cycle bending fatigue were carried out for AISI 1022 low-carbon steel after extreme thermal exposure, simulating the severe beyond-design-basis accident at nuclear power plants. In the as-delivered state, the steel has a high resistance to low-cycle fatigue (the fatigue strength at N = 3.5 × 104 cycles (σNf) was 360 MPa). Long-term thermal exposure led to a slight decrease in the resistance to low-cycle fatigue of steel: σNf is decreased by 9%. The influence of AISI 1022 steel structure on the characteristics of fatigue strength and fracture mechanisms is analyzed.
Antibacterial Effect and Mode of Action of Secondary Metabolites from Fungal Endophyte Associated with Aloe ferox Mill
Authors: Maliehe Tsolanku Sidney , Mbambo Melusi , Nqotheni Mduduzi Innocent , Senzo Ngema Siyanda , Shandu Jabulani Siyabonga Emmanuel
Published: February 2022

The constant increase in drug resistance, occurrence of incurable diseases and high medical costs, have necessitated bio-prospecting of fungi as alternative sources of therapeutic compounds. This study aimed at assessing the antibacterial effect and mode of action of secondary metabolites from fungal endophyte associated with Aloe ferox Mill. Endophytic fungus was isolated from the gel of A. ferox and identified by internal transcribed spacer (ITS) rRNA gene sequence analysis. The targets of antibacterial activity were assessed based on minimum inhibitory concentration (MIC) and the effect of the extract on respiratory chain dehydrogenase (RCD) and membrane integrity. Fourier transform-infrared spectrophotometer (FTIR) was employed to ascertain functional groups. The fungus with the most promising antibiotic-production was identified as Aspergillus welwitschiae MK450668.1. Its extract exhibited antibacterial activity with the MIC values of 0.5 and 1 mg/mL against Staphylococcus aureus (ATCC 25925) and Escherichia coli (ATCC 25922). It demonstrated the inhibitory effect on the RCD activity and destruction of membrane integrity on the test bacteria. FTIR spectrum revealed hydroxyl, amine and alkene groups. A. welwitschiae MK450668.1 serves as a potential source of effective compounds to combat the challenge of drug resistance.
Comprehensive Transcriptomic Analysis of Heterotrophic Nitrifying Bacterium Klebsiella sp. TN-10 in Response to Nitrogen Stress
Authors: Li Dan , Huang Mingquan , Dong Shirong , Jin Yao , Zhou Rongqing , Wu Chongde
Published: February 2022

Klebsiella sp. TN-10, a heterotrophic nitrifying bacterium, showed excellent nitrification ability under nitrogen stress. The strain was cultured under different nitrogen stress levels, including ammonium sulfate 0.5, 2.5, and 5 g/L, and samples were titled group-L, group-M, and group-H, respectively. In these three groups, the removed total nitrogen was 70.28, 118.33, and 157.18 mg/L after 12 h of cultivation, respectively. An RNA-Seq transcriptome analysis was used to describe key regulatory networks in response to nitrogen stress. The GO functional enrichment and KEGG enrichment analyses showed that differentially expressed genes (DEGs) participated in more pathways under higher nitrogen stress (group-H). Carbohydrate metabolism and amino acid metabolism were the most abundant subcategories, which meant these pathways were significantly influenced by nitrogen stress and could be related to nitrogen removal. In the nitrogen cycle, up-regulated gene2311 (narK, encodes major facilitator superfamily transporter) may accelerate the entry of nitrogen into the cells and subsequently contribute to the nitrogen utilization. In addition, the up-regulation of gene2312 (narG), gene2313 (narH), and gene2315 (narH) may accelerate denitrification pathways and facilitate nitrogen removal. The results presented in this study may play a pivotal role in understanding the regulation networks of the nitrifying bacterium TN-10 under nitrogen stress.
Spread of a Novel Indian Ocean Lineage Carrying E1-K211E/E2-V264A of Chikungunya Virus East/Central/South African Genotype across the Indian Subcontinent, Southeast Asia, and Eastern Africa
Authors: Phadungsombat Juthamas , Imad Hisham A. , Nakayama Emi E. , Leaungwutiwong Pornsawan , Ramasoota Pongrama , Nguitragool Wang , Matsee Wasin , Piyaphanee Watcharapong , Shioda Tatsuo
Published: February 2022

The Indian Ocean Lineage (IOL) of the chikungunya virus (CHIKV) East/Central/South African (ECSA) genotype, which originated in Kenya, spread to the Indian ocean and the Indian subcontinent, and then expanded through Southeast Asia in the previous decade. It carried an adaptive mutation E1-A226V, which enhances CHIKV replication in Aedes albopictus. However, the IOL CHIKV of the most recent outbreaks during 2016–2020 in India, Pakistan, Bangladesh, the Maldives, Myanmar, Thailand, and Kenya lacked E1-A226V but carried E1-K211E and E2-V264A. Recent CHIKV genome sequences of the Maldives and Thailand were determined, and their phylogenetic relationships were further investigated together with IOL sequences reported in 2004–2020 in the database. The results showed that the ancestral IOLs diverged to a sub-lineage E1-K211E/E2-V264A, probably in India around 2008, and caused sporadic outbreaks in India during 2010–2015 and in Kenya in 2016. The massive expansion of this new sub-lineage occurred after the acquisition of E1-I317V in other neighboring and remote regions in 2014–2020. Additionally, the phylogenetic tree indicated that independent clades formed according to the geographical regions and introduction timing. The present results using all available partial or full sequences of the recent CHIKVs emphasized the dynamics of the IOL sub-lineages in the Indian subcontinent, Southeast Asia, and Eastern Africa.
Physiological Effects of 2-Bromoethanesulfonate on Hydrogenotrophic Pure and Mixed Cultures
Authors: Logroño Washington , Nikolausz Marcell , Harms Hauke , Kleinsteuber Sabine
Published: February 2022

Mixed or pure cultures can be used for biomethanation of hydrogen. Sodium 2-bromoethanesulfonate (BES) is an inhibitor of methanogenesis used to investigate competing reactions like homoacetogenesis in mixed cultures. To understand the effect of BES on the hydrogenotrophic metabolism in a biomethanation process, anaerobic granules from a wastewater treatment plant, a hydrogenotrophic enrichment culture, and pure cultures of Methanococcus maripaludis and Methanobacterium formicicum were incubated under H2/CO2 headspace in the presence or absence of BES, and the turnover of H2, CO2, CH4, formate and acetate was analyzed. Anaerobic granules produced the highest amount of formate after 24 h of incubation in the presence of BES. Treating the enrichment culture with BES led to the accumulation of formate. M. maripaludis produced more formate than M. formicicum when treated with BES. The non-inhibited methanogenic communities produced small amounts of formate whereas the pure cultures did not. The highest amount of acetate was produced by the anaerobic granules concomitantly with formate consumption. These results indicate that formate is an important intermediate of hydrogenotrophic metabolism accumulating upon methanogenesis inhibition.
The Response Regulator FlmD Regulates Biofilm Formation in Comamonas testosteroni through the Transcriptional Activator SoxR
Authors: Wang Yunhao , Huang Zhou , Zhou Nan , Liu Chang , Jiang Chengying , Li Defeng , Liu Shuangjiang
Published: February 2022

Biofilm formation is a survival strategy by which microorganisms adapt to environmental challenges. It is regulated by various signals, such as the second messenger c-di-GMP. We previously found that the Flm chemosensory pathway could respond to chemical signals and regulate biofilm formation. This regulation is independent of c-di-GMP. A previous study revealed that the response regulator FlmD is involved in biofilm formation; however, how chemical signals are transmitted downstream of FlmD remained unclear. In the present study, transcriptome analysis and gel shift assay reveal that SoxR, a transcriptional activator of the efflux transporter acrAB-tolC operon, mediates the downstream signaling of FlmD. Phosphorylated FlmD interacts with SoxR and disrupts the interaction between SoxR and the acrAB-tolC operon. It causes a decrease in the expression of acrAB-tolC operon. The downregulation of acrA, acrB, or tolC gene expression results in making less biofilm formation. In conclusion, we identified that the transcription regulator SoxR plays a role in the c-di-GMP independent regulation of biofilm formation in Comamonas testosteroni.
Effects of Different pH Control Strategies on Microalgae Cultivation and Nutrient Removal from Anaerobic Digestion Effluent
Authors: Yu Hyeonjung , Kim Jaai , Rhee Chaeyoung , Shin Juhee , Shin Seung Gu , Lee Changsoo
Published: February 2022

This study investigated nutrient removal from anaerobic digestion effluent by cultivating mixed-culture microalgae enriched from anaerobic sludge under different pH conditions: RUC (uncontrolled), R7–8 (maintained at 7–8), and R<8 (maintained below 8). Significant amounts of NH4+-N were lost by volatilization in RUC cultures due to increased pH values (≤8.6) during the early period of cultivation. The pH control strategies significantly affected the biological NH4+-N removal (highest in R7–8), microalgal growth (highest in R7–8), biomass settleability (highest in R<8), and microalgal growth relative to bacteria (highest in R<8) in the cultures. Parachlorella completely dominated the microalgal communities in the inoculum and all of the cultures, and grew well at highly acidic pH (<3) induced by culture acidification with microalgal growth. Microalgae-associated bacterial community structure developed very differently among the cultures. The findings call for more attention to the influence and control of pH changes during cultivation in microalgal treatment of anaerobic digestion effluent.
Translational Fusion to Hmp Improves Heterologous Protein Expression
Authors: Wan Xuanqing , Link A. James , Brynildsen Mark P.
Published: February 2022

Flavohemoglobins, which are widely distributed in prokaryotes and eukaryotes, play key roles in oxygen (O2) transport and nitric oxide (·NO) defense. Hmp is the flavohemoglobin of Escherichia coli, and here we report that the translational fusion of Hmp to the N-terminus of heterologous proteins increases their expression in E. coli. The effect required the fusion of the proteins, and was independent of both the O2-binding and catalytic activity of Hmp. Increased expression was at the translational level, likely to be downstream of initiation, and we observed that as little as the first 100 amino acids of Hmp were sufficient to boost protein production. These data demonstrate the potential of Hmp as an N-terminal fusion tag to increase protein yield, and suggest that the utility of bacterial hemoglobins to biotechnology goes beyond their O2 transport and ·NO detoxification capabilities.
Unraveling the Uncharacterized Domain of Carocin S2: A Ribonuclease Pectobacterium carotovorum subsp. carotovorum Bacteriocin
Authors: Chung Ping-Chen , Lagitnay Ruchi Briam James S. , Derilo Reymund C. , Wu Jian-Li , Chuang Yutin , Lin Jia-De , Chuang Duen-Yau
Published: February 2022

Carocin S2 is a bacteriocin with a low molecular weight generated by Pectobacterium carotovorum subsp. carotovorum 3F3 strain. The caroS2K gene, which is found in the genomic DNA alongside the caroS2I gene, which codes for an immunity protein, encodes this bacteriocin. We explored the residues responsible for Carocin S2’s cytotoxic or RNA-se activity using a structure-based mutagenesis approach. The minimal antibiotic functional region starts at Lys691 and ends at Arg783, according to mutational research. Two residues in the identified region, Phe760 and Ser762, however, are unable to demonstrate this activity, suggesting that these sites may interact with another domain. Small modifications in the secondary structure of mutant caroS2K were revealed by circular dichroism (CD) spectroscopy and intrinsic tryptophan fluorescence (ITF), showing ribosomal RNA cleavage in the active site. A co-immunoprecipitation test indicated that the immunity protein CaroS2I binds to CaroS2K’s C-terminus, while a region under the uncharacterized Domain III inhibits association of N-terminally truncated CaroS2K from interacting with CaroS2I. Carocin S2, a ribosomal ribonuclease bacteriocin, is the first to be identified with a domain III that encodes the cytotoxic residues as well as the binding sites between its immunity and killer proteins.
Endophytic Fungi: Key Insights, Emerging Prospects, and Challenges in Natural Product Drug Discovery
Authors: Tiwari Pragya , Bae Hanhong
Published: February 2022

Plant-associated endophytes define an important symbiotic association in nature and are established bio-reservoirs of plant-derived natural products. Endophytes colonize the internal tissues of a plant without causing any disease symptoms or apparent changes. Recently, there has been a growing interest in endophytes because of their beneficial effects on the production of novel metabolites of pharmacological significance. Studies have highlighted the socio-economic implications of endophytic fungi in agriculture, medicine, and the environment, with considerable success. Endophytic fungi-mediated biosynthesis of well-known metabolites includes taxol from Taxomyces andreanae, azadirachtin A and B from Eupenicillium parvum, vincristine from Fusarium oxysporum, and quinine from Phomopsis sp. The discovery of the billion-dollar anticancer drug taxol was a landmark in endophyte biology/research and established new paradigms for the metabolic potential of plant-associated endophytes. In addition, endophytic fungi have emerged as potential prolific producers of antimicrobials, antiseptics, and antibiotics of plant origin. Although extensively studied as a “production platform” of novel pharmacological metabolites, the molecular mechanisms of plant–endophyte dynamics remain less understood/explored for their efficient utilization in drug discovery. The emerging trends in endophytic fungi-mediated biosynthesis of novel bioactive metabolites, success stories of key pharmacological metabolites, strategies to overcome the existing challenges in endophyte biology, and future direction in endophytic fungi-based drug discovery forms the underlying theme of this article.
Reactivation of Herpes Simplex Virus Type 1 (HSV-1) Detected on Bronchoalveolar Lavage Fluid (BALF) Samples in Critically Ill COVID-19 Patients Undergoing Invasive Mechanical Ventilation: Preliminary Results from Two Italian Centers
Authors: Giacobbe Daniele Roberto , Di Bella Stefano , Dettori Silvia , Brucci Giorgia , Zerbato Verena , Pol Riccardo , Segat Ludovica , D’Agaro Pierlanfranco , Roman-Pognuz Erik , Friso Federica , Principe Luigi , Lucangelo Umberto , Ball Lorenzo , Robba Chiara , Battaglini Denise , De Maria Andrea , Brunetti Iole , Patroniti Nicolò , Briano Federica , Bruzzone Bianca , Guarona Giulia , Magnasco Laura , Dentone Chiara , Icardi Giancarlo , Pelosi Paolo , Luzzati Roberto , Bassetti Matteo
Published: February 2022

Reactivation of herpes simplex virus type 1 (HSV-1) has been described in critically ill patients with coronavirus disease 2019 (COVID-19) pneumonia. In the present two-center retrospective experience, we primarily aimed to assess the cumulative risk of HSV-1 reactivation detected on bronchoalveolar fluid (BALF) samples in invasively ventilated COVID-19 patients with worsening respiratory function. The secondary objectives were the identification of predictors for HSV-1 reactivation and the assessment of its possible prognostic impact. Overall, 41 patients met the study inclusion criteria, and 12/41 patients developed HSV-1 reactivation (29%). No independent predictors of HSV-1 reactivation were identified in the present study. No association was found between HSV-1 reactivation and mortality. Eleven out of 12 patients with HSV-1 reactivation received antiviral therapy with intravenous acyclovir. In conclusion, HSV-1 reactivation is frequently detected in intubated patients with COVID-19. An antiviral treatment in COVID-19 patients with HSV-1 reactivation and worsening respiratory function might be considered.
Antifungal Properties of Chemically Defined Propolis from Various Geographical Regions
Authors: Ożarowski Marcin , Karpiński Tomasz M. , Alam Rahat , Łochyńska Małgorzata
Published: February 2022

Long-term fungal infections that are difficult to treat require new substances for their prevention, treatment, or as adjuvants during antibiotic therapy. Propolis is a very promising source of natural substances that show a wide range of pharmacological properties, including antifungal activity against various fungal strains. The purpose of the literature review was to summarize recent studies (PubMed, Scopus) on progress in evaluating the antifungal activity of chemically defined propolis extracts. During the selection of studies, only those with results of antifungal activity expressed as minimal inhibitory concentration (MIC) and/or minimal fungicidal concentration (MFC) were analyzed. Moreover, plant, animal and environmental factors influencing the chemical composition of propolis are discussed. Mechanisms of antifungal activity of propolis extracts and research trends in the aspect of developing new therapies and the assessment of drug interactions are indicated. The review of the research results shows that there is great progress in the definition of propolis extracts. After comparing the MIC/MFC values, it was assessed that propolis extracts offer a wide range of activity not only against pathogenic Candida strains but also against risky molds; however, the strength of this activity is varied.
Proteomic Analysis of Spore Surface Proteins and Characteristics of a Novel Spore Wall Protein and Biomarker, EhSWP3, from the Shrimp Microsporidium Enterocytozoon hepatopenaei (EHP)
Authors: Fan Xiaodong , Wei Chunmei , Yang Xiaojuan , Xiao Ai , Tan Nianqiu , Chen Jie , Long Mengxian , Pan Guoqing , Wan Yongji , Zhou Zeyang
Published: February 2022

Enterocytozoon hepatopenaei, a spore-forming and obligate intracellular microsporidium, mainly infects shrimp and results in growth retardation and body length variation, causing huge economic losses to the Asian shrimp aquaculture industry. However, the lack of a full understanding of the surface proteins of spores associated with host infection has hindered the development of technologies for the detection of EHP. In this study, the surface proteins of EHP spores were extracted using the improved SDS method, and 130 proteins were identified via LC-MS/MS analysis. Bioinformatic analysis revealed that these proteins were enriched in biological processes (67), cellular components (62), and molecular functions (71) based on GO terms. KEGG pathway analysis showed that 20 pathways, including the proteasome (eight proteins) and the fatty acid metabolism (15 proteins), were enriched. Among 15 high-abundance surface proteins (HASPs), EhSWP3 was identified as a novel spore wall protein (SWP), and was localized on the endospore of the EHP spores with an indirect immunofluorescence and immunoelectron microscopy assay. Polyclonal antibodies against EhSWP3 showed strong species specificity and high sensitivity to the hepatopancreas of EHP-infected shrimp. As a specific high-abundance protein, EhSWP3 is therefore a promising target for the development of immunoassay tools for EHP detection, and may play a crucial role in the invasion of EHP into the host.
Authigenic Green Mica in Interflow Horizons within Late Cretaceous Deccan Volcanic Province, India and Its Genetic Implications
Authors: Singh Pragya , Banerjee Santanu , Pande Kanchan , Bhattacharya Satadru , Sarkar Subham , Le Pera Emilia
Published: February 2022

Green authigenic mica, i.e., celadonite, is commonly associated with submarine alteration of basic igneous rock. However, very few studies have reported the formation of celadonite under nonmarine conditions. An integrated study involving field investigation, petrography, mineralogy, and mineral chemistry highlighted the origin of celadonite in two clay-rich horizons (green boles) of the Late Cretaceous Deccan volcanic province. Within the Salher green bole, the celadonite occurred as the dissolution and alteration of plagioclase, volcanic glass, and pore-filling cement. In the case of the Pune green bole, the celadonite was formed by the alteration of plagioclase, pyroxene, and precipitation as film within intergranular pores, along with zeolite. The celadonite in the Salher green bole exhibited slightly lower K2O and Fe2O3 and higher Al2O3 than in the Pune. The mineral chemistry of the former showed a composition closer to ferro-aluminoceladonite. Although the mineral chemistry of celadonite overlaps with glauconite, the distinct 10 Å and 15 Å reflections in XRD, euhedral lath and honeycomb morphology under SEM, and characteristic absorption bands in VNIR spectroscopy (0.4–2.5 µm) and FTIR spectroscopy (400–4000 cm−1) identified celadonite and Fe-smectite within green boles. The green boles were formed either by the alteration of a volcaniclastic deposit in local pools of water or by the in situ alteration of the fragmentary flow top. The present study is significant due to the occurrence of celadonite in a nonmarine environment, as it otherwise forms under submarine conditions.
Reassessing Depositional Conditions of the Pre-Apulian Zone Based on Synsedimentary Deformation Structures during Upper Paleocene to Lower Miocene Carbonate Sedimentation, from Paxoi and Anti-Paxoi Islands, Northwestern End of Greece
Authors: Bourli Nicolina , Iliopoulos George , Zelilidis Avraam
Published: February 2022

The studied area is situated in northwestern Greece and corresponds to the northern end of the Pre-Apulian Zone, in contact with the Apulian platform to the west and the Ionian Basin to the east. The proposed model is based on fieldwork, measured deformation structures, and age determination of the studied deposits. Until now, the known Pre-Apulian platform or Pre-Apulian zone represents the margins of the Apulian platform to the Ionian Basin and was formed due to the normal faults’ activity during the Mesozoic to Cenozoic Eras. Soft sediment deformation (SSD) structures are widespread within the upper Paleocene to lower Miocene limestones/marly limestones that are exposed in both Paxoi and Anti-Paxoi Islands, mostly along their eastern coasts, across sections of 2–3 km long and up to 60 m high. SSD structures, with a vertical thickness up to 10 m, have been observed in limestones and were formed during or immediately after deposition, during the first stage of sediment consolidation. SSD structures are cross-cut by normal faults, indicating their development during the rift stage. There are at least five different SSD horizons, and most of them present either an eastward or a westward progradation. These SSD structures are classified into four (4) different types of deformations: (1) thick synclines and anticlines, formed due to strong synsedimentary deformation; (2) strong and thick SSD structures that produced erosional contacts both with the underlying and the overlying undeformed horizons; (3) thin slumps, having sharp contacts with the underlying undeformed horizons and erosional contacts with the overlying undeformed horizons; and (4) thin slump horizons passing laterally to undeformed deposits in the same horizon. The studied SSD structures and their age of development introduce active margins between the Apulian platform and the Ionian Basin that have been influenced by normal fault activity. These normal faults have been active since the Ionian Basin changed gradually to a foreland basin, and after the tectonic regime changed from extension to compression, during the early to middle Eocene. It seems that compression in the studied Apulian platform margins arrived later and after the lower Miocene, and after the development of the SSD structures. The confinement of the lower Miocene deposits, both northwards and southwards (in Anti-Paxoi Island), indicates the presence of active transfer faults, with flower structure geometry, that were formed during sedimentation, producing highs and troughs. The present open anticline geometry of Paxoi Island indicates that the Island represents the forebulge area of the middle Miocene Ionian Foreland due to Ionian Thrust activity.
Oxidative Stress and Free Radical Processes in Tumor and Non-Tumor Obstructive Jaundice: Influence of Disease Duration, Severity and Surgical Treatment on Outcomes
Authors: Silina Ekaterina Vladimirovna , Stupin Victor Alexandrovich , Abramov Igor Sergeevich , Bolevich Sergey Brankovich , Deshpande Gouri , Achar Raghu Ram , Sinelnikova Tatiana Georgievna
Published: February 2022

The aim of this study was to assess the patterns and pattern disruptions of free radical processes in patients with obstructive jaundice of various origins, and the severity of jaundice before and after decompression. Oxidative stress markers were determined in 128 patients with obstructive jaundice with a tumor genesis (23.4%) or non-tumor genesis (76.6%). The patients were hospitalized at different stages of clinical signs of jaundice. We studied the anti-peroxide activity in plasma, basal and stimulated indicators of the chemiluminescence intensity in leukocytes, leukocyte activity coefficients reflecting the level of reactive oxygen species generated by leukocytes, malondialdehyde levels indicative of the degree of lipid peroxidation and cellular destruction, liver enzymes (markers of cytolysis) and bilirubin levels. Data for hepatocyte death and markers of oxidative stress correlated with the severity of jaundice, its duration and the method of its surgical correction. It is proposed that using markers of free radical processes to assess the prognosis and effectiveness of treatment and to personalize treatment measures will improve the results of jaundice treatment.
HUBUNGAN MOTIVASI ORANG TUA DENGAN KEBERADAAN TELUR CACING PADA TINJA SISWA DI SDN 1 SUKAWATI KABUPATEN GIANYAR
Authors: Dwiutami Anak Agung Ary , Rusminingsih Ni Ketut , Aryana I Ketut
Published: February 2022

Bali province is one of provinces in Indonesia whose community especially children aged 5-12 years who still have intestinal worms infection with varying prevalence. This research is done in order to know the relationship of parents' motivation with the existence of worm eggs in the stools of students at SDN 1 Sukawati. This research is an analytical survey with cross sectional study design. The number of samples from this study are 67 respondents and 67 samples of stool students. Statistical test analysis in this study using Chi Square analysis method (X2). Characteristics of respondents obtained the dominant of age parents and students are between 38-43 years (52%) and students between 9-11 years (48%). The dominant of parents' education and the sex of the students are senior high school (47%) and female (52%). The relationship of parents' motivation with the existence of worm eggs in the stools of students is undefined because the existence of worm eggs in stool students is constant or all (100%) of samples stool are negative worm eggs. Various factors other than motivation can influence it. Nevertheless, parents should still motivate students to prevent intestinal worms because the disease can be infected at any time.
Perbedaan Kandungan Formalin Pada Jenis Ikan Asin Peda Dan Gabus Di Pasar Tradisional Kota Denpasar Tahun 2019
Authors: Sukmadhani Ni Made Meri , Sudiadnyana I Wayan
Published: February 2022

In the food processing business it is known that the use of preservatives to maintain the durability of a food ingredient, but in practice there are still many producers who use dangerous preservatives such as formalin in food products, one of which is in the salted long jawed mackerel (Rastrelliger sp.) and salted Asian Snakehead (Channa striata). The purpose of this study was to determine the differences in formalin content in the salted long jawed mackerel and salted Asian Snakehead fish This study used an analytical survey with a cross sectional design. The samples were then examined using the Formaldehyde Test method. The results of this study indicate that from 162 samples of the fishes examined, 19 salted mackerels (23.5%) and 39 salted snakeheads (48.1%) were free of formalin and fulfilled the health requirements with a formalin content of 0 mg / l. The conclusion of this study is that there are differences in formalin content in the salted mackerels and salted snakeheads sold in the traditional market in Denpasar in 2019.
Piper aduncum L. (matico) utilizado como tratamiento para el daño pulmonar y Covid-19
Authors: Ore Areche Franklin , Ticsihua Huaman Jovencio , Ruiz Rodríguez Alfonso , Corilla Flores Denis Dante
Published: February 2022

El objetivo principal fue conocer las propiedades antimicrobianas del Piper aduncum L. (matico) para el tratamiento de prevención del Covid-19. El aceite esencial, como los extractos de las hojas y la corteza son muy valiosos como agente cicatrizante, antibacterianos y antifúngico. De esta manera los componentes los flavonoides presentes en el aceite esencial de Piper aduncum L. (matico) fortifican la conectividad de los tejidos mediante la inhibición de algunas de las enzimas que pueden hidrolizar el proteoglicano y la malla proteica, lo cual podría aumentar el efecto antibacteriano al obstaculizar la difusión de la infección al tejido, su actividad antibacterial frente a Staphylococus aureus y Echerichia coli; obteniéndose como resultado que los metabolitos secundarios como flavonoides, compuestos fenólicos, esteroides y alcaloides cuentan con acción antibacterial. La planta del Piper aduncum L. (matico) es un buen cicatrizante y antifúngico se está comercializando como producto farmacéutico en gel. La conclusión que el aceite esencial de Piper aduncum L. (matico) no ayuda a prevenir ni mucho menos a eliminar el nuevo coronavirus y su composición química del matico solo ayuda a prevenir algunas de los síntomas del Covid 19.
A propos d’un aspect méconnu de la législation minière athénienne au IVe s. av. n. ère
Authors: Flament Christophe
Published: February 2022

Cette étude propose une nouvelle interprétation de l’infraction libellée «(ἐπικατα) τέμνω τῶν μέτρων ἐντός » dans Démosthène, XXXVII, 36 et Hypéride, III, 35. Contrairement à l’opinion reçue, cette infraction n’aurait pas consisté à dépasser les limites assignées à sa concession, mais à ne pas respecter les dimensions minimales que la Cité imposait pour le creusement des galeries.
PENGARUH SIKLUS OPERASIONAL, RESIKO HUTANG DAN UKURAN PERUSAHAAN TERHADAP PERSISTENSI LABA
Authors: Nainggolan Piter
Published: February 2022

The purpose of this study was to examine the effect of operating cash flow, debt levels, and firm size on earnings persistence. Earnings persistence reflects the quality of a company's earnings, which indicates that the company can maintain profit in its activities. This study used secondary data and the research sample is determined by purposive sampling method. The sample which used in this study were 8 retail goods companies listed on the Indonesia Stock Exchange (BEI) for the period of 2016 until 2019. The analysis in this study used the SPSS 25 program where the statistical model used was multiple regression analysis.Based on the research results, the conclusion obtained is that the level of company’s debt and company’s size partially has no effect on earnings persistence. Operating cashflow partially affects earnings persistence.

Keywords: Background of the board of commissioners, citizenship of the board of commissioners, independent board of commissioners, audit committee
PENGARUH BUDAYA ORGANISASI DAN KOMUNIKASI TERHADAP KINERJA KARYAWAN PADA PT. THE SERVICE LINE (SOS) PROJECT GEDUNG GRAHA ADHI MEDIA JAKARTA
Authors: Surono Surono , Suhirto Suhirto
Published: February 2022

This study aims to obtain information to a deeper explanation of  influence of organizational culture and communication on employee performance. And get the results from data processing about how much influence organizational culture and communication have on employee performance. The research method used is quantitative method, which is a method for studying the object of research by trying to explain the relationship between research variables using statistical calculations. Based on the analysis of the multiple linear regression equation between X1, X2, and Y is Y = 25.475 + 0.098X1 + (-0.0320X2) ……… e, this means that if there is an increase in Organizational Culture by 1 point, Employee Performance will increase to equal 1 point, the employee's performance will increase to 25.475 + 0.098 (1) = 25.573 and if there is an increase in communication by 1 point, the employee's performance will increase to 25.475 + (-0.032) (1) = 25.443. While the results of hypothesis testing with the t test, the results obtained from the organizational culture variable, the value of sig> 0.05 means not significant, while for the communication variable the value of sig> 0.05 means not significant. For the results of the organizational culture variable, the t value = 0.333 and t table = 1.688 and the results of the communication variable, the t value = -0.150 and t table = 1.688, meaning that partially, influence organizational culture has a positive and insignificant relationship between communication variables and employee performance. , while for the Communication variable there is no negative but insignificant relationship to employee performance. Simultaneously proving the hypothesis that Organizational Culture and Communication has an effect on employee ability where F count is less than F table (0.057 <3.26), which means that organizational culture and communication variables together have no and insignificant effect on employee ability. And based on the correlation coefficient analysis, the value of r = 0.57 shows that this value means that the two variables have a strong correlation (relationship). While the results of the coefficient of determination (R2) = 0.003, meaning 0.3%, while the remaining 99.7% is independent variables not not researched

Key Words: Organizational Culture, Communication And Employee Performance
Моделювання процесу теплообміну між основними агрегатами сучасних колісних бронетранспортерів на базі методів кінцевоелементного моделювання
Authors: Жадан Володимир Андрійович , Ларін Олександр Юрійович , Майстренко Олександр Анатолійович , Почечун Олександр Олексійович
Published: February 2022

В статті на основі математичних розрахунків та отриманих експериментальним шляхом даних проведено дослідження компонувальних рішень моторно-трансмісійного відділення (МТВ), гідросистем бронетранспортера з вентиляторною системою охолодження. Ці матеріали мають практичну цінність і повинні використовуватися на початкових етапах проєктування систем охолодження основних елементів бронетранспортера з метою їх оптимізації та поліпшення характеристик.В статті авторами проаналізовано існуючі конструкції колісних бронетранспортерів на базі методів кінцевоелементного моделювання фізичних процесів, зокрема, процесів теплообміну в системі охолодження сучасних зразків військової техніки вітчизняного виробництва БТР-4А, БТР-4В і БТР-4Е-2. При попередніх оцінках варіантів компонування моторно-трансмісійного відсіку, гідропневматичною підвіски і вентиляторної системи охолодження силової установки представлена математична модель теплових потоків дозволяє з достатньою точністю визначити ефективність пропонованих перспективних зразків.
Social-Economic Effects and Political Satisfaction from Pedestrian Footbridges in Rural Areas
Authors: Munyaneza Claude , Mbereyaho Leopold
Published: February 2022

It is generally accepted that having a proper river crossing point has a positive effect on rural inhabitants. Such crossing points would increase the ability of communities to access their social services, markets, jobs, and thereby lead to raising their standard of living. In line with this objective,Rwanda has been promoting the construction of pedestrian footbridges in rural areas, and with different partners, different pedestrian footbridges have already been constructed. It is very crucial that all institutions involved in the construction of pedestrian footbridges, both public and private, consider the financial benefits of pedestrian footbridges and some significant direct and indirect effects on the rural communities.The Objective of this study was to identify all possible benefits that may be generated by pedestrian footbridges to understand the potential range of their impacts in rural areas and the likely responses from those people impacted by the project. The study also proposed a comprehensive approach for estimating the economic impacts of a pedestrian footbridge in rural areas. The methodology involved community interviews conducted during site visits to identify and predict possible impacts due to the lack and availability of safe access via pedestrian footbridges.
General Approach to Diagnostics and Differential Diagnostics by Exants in Children
Authors: Романова О.Н. , Довнар-Запольская О.Н. , Манкевич Р.Н. , Преображенская О.А. , Сергиенко Е.Н. , Матуш Л.И. , Дивакова Е.В. , Ткаченко А.К. , Твардовский В.И. , Ханенко О.Н. , Тонко О.В. , Симанкова О.С. , Кастюкевич Л.И. , Коломиец Н.Д.
Published: February 2022

Известно немало заболеваний у детей, которые сопровождаются поражением кожи в виде экзантем, являющихся полиэтиологичными по происхождению и полиморфными по элементам сыпи и характеру распространения. При ведении таких пациентов нередко возникают значительные дифференциально-диагностические затруднения у педиатров, зачастую требующие консультации врача-инфекциониста. В статье дана характеристика основных видов сыпи у детей, ее диагностическое и дифференциально-диагностическое значение. Сыпь, или экзантема, - наиболее частый симптом, с которым обращаются родители за помощью к педиатру. Экзантемы характеризуются полиморфизмом этиологических и клинических проявлений, однако постановка диагноза в амбулаторных условиях проводится, как правило, на основании только клинической картины без лабораторного подтверждения, что нередко вызывает большие трудности у педиатров. В диагностическом процессе очень важно четкое описание морфологических элементов, появляющихся на коже.

Many diseases in children accompanied by skin lesions in the form of exanthema, which are polyetiologic in origin and polymorphic in terms of the elements of the rash and the nature of their distribution. Pediatricians often have significant differential diagnostic difficulties, requiring the consultation of an infectious disease doctor in the management of such patients. The article describes the characteristics of the main types of rash in children, its diagnostic and differential diagnostic value. A rash or exanthema is the most common symptom with which parents seek help from a pediatrician. Exanthema is characterized by polymorphism of etiological and clinical manifestations, however, the diagnosis on an outpatient basis is usually carried out on the basis of only the clinical picture without laboratory confirmation, which often causes great difficulties for pediatricians. A clear description of the morphological elements appearing on the skin is very important in the diagnostic process.
Issues of Utilization of Videomotion Analysis and Stabilometry with Force Platform in the Study of Voluntary Postural Sway
Authors: Гурский И.С. , Лихачев С.А. , Марьенко И.П. , Рушкевич Ю.Н. , Буняк А.Г.
Published: February 2022

Введение. Частым проявлением нейродегенеративных заболеваний является нарушение локомоции и поддержания равновесия, что снижает качество жизни пациентов и может стать причиной летального исхода (например, при тяжелой ЧМТ, переломе бедра). Цель. Выявить особенности применения видеоанализа движений и стабилометрии с использованием стабилоплатформы для изучения произвольного постурального контроля. Материалы и методы. Использовались аппаратно-программный комплекс для осуществления анализа движений и биологической обратной связи на основе метода видеоанализа движений и стабилоплатформа «Стабилан-01-2». Обследовано 4 пациента с болезнью Паркинсона и 2 здоровых субъекта. В процессе выполнения испытуемым задач на произвольный постуральный контроль проводилась параллельная регистрация движений туловища методом видеоанализа и перемещений центра давления при помощи стабилоплатформы. Результаты. У испытуемых, лучше выполнявших пробу, колебания центра давления в диапазоне частот от 0,25 до 1,0 Гц не сопровождались такими же выраженными колебаниями туловища, что отражает эффективный и упреждающий характер сокращений мышц нижних конечностей, направленных на выполнение движения туловища в соответствии с заданным паттерном. Заключение. Методики видеоанализа движений и стабилографии с использованием стабилоплатформы позволяют оценить связанные между собой, однако различающиеся по физическому смыслу параметры поддержания вертикальной позы и произвольного постурального контроля, а их параллельное применение помогает получить более целостную картину явления.

Introduction. Impairment of balance and locomotion is a common manifestation of neurodegenerative disorders, that impairs patients’ quality of life, and can be a cause of mortality (e.g., due to severe head trauma, or hip fracture). Purpose. To assess the issues of utilization of videomotion analysis and stabilometry with force platform in the study of voluntary postural sway. Materials and methods. We studied 4 patients with Parkinson’s disease, and 2 healthy volunteers. The subjects performed voluntary postural sway tasks under the guidance of videomotion analysis with visual feedback, and concurrently center of pressure position was measured using the force platform. Results. The subjects, who better performed the task, had less power of trunk oscillations in frequency range from 0.25 to 1.0 Hz, compared to oscillations of the center of pressure, that reflect an effective and anticipatory way of muscle contractions, that direct the body sway according to the given pattern. Conclusion. Therefore, videomotion analysis and stabilometry with force platform can assess connected, but having different physical meaning, parameters of balance and voluntary postural sway; their concurrent utilization helps to achieve more universal picture of the phenomena.