bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative Assessment of the State of Threat of Working on Construction Scaffolding
Authors: Sawicki Marek , Szóstak Mariusz
Published: August 2020

Working at height, and especially on construction scaffolding, is one of the most accident-prone situations on a construction site. The article attempts to assess the state of threat of working on scaffolding on the basis of the proposed coefficients concerning the possibility of an occupational accident occurring. The article presents the analysis of 10 parameters, which were classified into three groups of factors that cause accidents: technical, organizational, and human factors. In order to assess the state of threat of working on scaffolding, partial hazard factors and a simplified and accurate factor of the state of threat of working were proposed. The coefficients were determined on the basis of the data collected from post-accidental control reports on occupational accidents occurring on scaffolding in the construction industry that took place in Poland in five voivodeships in the years 2008–2017, and also on the basis of the obtained results of research on 120 scaffoldings conducted in the years 2016–2018. Based on the determined factors, it was possible to determine the probability of an undesirable event, in other words, an occupational accident. In addition, the developed test method proposed numerical scales for assessing the state of threat of working on scaffolding. The form proposed in the article for assessing the state of threat of working on scaffolding, which was developed using a spreadsheet, can provide support for people managing work at workstations involving scaffolding, for example, construction directors, construction engineers, work managers, or construction managers.
Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore
Authors: Aung Kyaw Thu , Khor Wei Ching , Octavia Sophie , Ye Agnes , Leo Justina , Chan Pei Pei , Lim Georgina , Wong Wai Kwan , Tan Brian Zi Yan , Schlundt Joergen , Dalsgaard Anders , Ng Lee Ching , Lin Yueh Nuo
Published: August 2020

We analyzed the epidemiological distribution of Salmonella serovars in humans, foods, animals and the environment as a One-Health step towards identifying risk factors for human salmonellosis. Throughout the 2012–2016 period, Salmonella ser. Enteritidis was consistently the predominating serovar attributing to >20.0% of isolates in humans. Other most common serovars in humans include Salmonella ser. Stanley, Salmonella ser. Weltevreden, Salmonella ser. Typhimurium and Salmonella ser. 4,5,12:b:-(dT+). S. Enteritidis was also the most frequent serovar found among the isolates from chicken/chicken products (28.5%) and eggs/egg products (61.5%) during the same period. In contrast, S. Typhimurium (35.2%) and Salmonella ser. Derby (18.8%) were prevalent in pork/pork products. S. Weltevreden was more frequent in seafood (19.2%) than others (≤3.0%). Most isolates (>80.0%) from farms, companion and wildlife animals belonged to serovars other than S. Enteritidis or S. Typhimurium. Findings demonstrate the significance of a One-Health investigative approach to understand the epidemiology Salmonella for more effective and integrated surveillance systems.
Development of a Survivorship Care Plan (SCP) Program for Rural Latina Breast Cancer Patients: Proyecto Mariposa—Application of Intervention Mapping
Authors: Ko Eunjeong , Zúñiga María Luisa , Woodruff Susan I. , Serra-Martinez Yolanda , Cardenas Veronica
Published: August 2020

Latina breast cancer survivors are less likely to receive a comprehensive Survivorship Care Plan (SCP) than non-Latina Whites. Evidence-based and theory driven interventions are needed to promote a culturally and linguistically responsive SCP. This paper describes the application of Intervention Mapping (IM) in the development of Proyecto Mariposa, a comprehensive SCP program targeting Latina breast cancer survivors living in a rural U.S.–Mexico border region. We conducted a needs assessment using focus groups (n = 40) and individual interviews (n = 4) with stakeholders to elicit their needs and preferences relating to SCPs and SCP aid (Step1). Content analysis of transcripts was conducted using Atlas.ti. The findings informed the development of a matrix of change objectives where we selected specific behavioral theories to ground the practical application of the SCP program (Step 2). We identified behavioral theories and the practical application of behavioral change (Step 3) and designed and developed a comprehensive SCP program which consisted of a culturally—and linguistically—adapted SCP document and animated video as an SCP aid (Step 4). The systematic application of the IM framework resulted in the development of a comprehensive and culturally tailored SCP intervention. Stakeholder active involvement in the cultural tailoring of the program was imperative and strengthens the SCP intervention.
An Integrated Strategy for the Prevention of SARS-CoV-2 Infection in Healthcare Workers: A Prospective Observational Study
Authors: Cattelan Anna Maria , Sasset Lolita , Di Meco Eugenia , Cocchio Silvia , Barbaro Francesco , Cavinato Silvia , Gardin Samuele , Carretta Giovanni , Donato Daniele , Crisanti Andrea , Trevenzoli Marco , Baldo Vincenzo
Published: August 2020

Background: Since the beginning of SARS-CoV-2 outbreak, a large number of infections have been reported among healthcare workers (HCWs). The aim of this study was to investigate the occurrence of SARS-CoV-2 infection among HCWs involved in the first management of infected patients and to describe the measures adopted to prevent the transmission in the hospital. Methods: This prospective observational study was conducted between February 21 and April 16, 2020, in the Padua University Hospital (north-east Italy). The infection control policy adopted consisted of the following: the creation of the “Advanced Triage” area for the evaluation of SARS-CoV-2 cases, and the implementation of an integrated infection control surveillance system directed to all the healthcare personnel involved in the Advance Triage area. HCWs were regularly tested with nasopharyngeal swabs for SARS-CoV-2; body temperature and suggestive symptoms were evaluated at each duty. Demographic and clinical data of both patients and HCWs were collected and analyzed; HCWs’ personal protective equipment (PPE) consumption was also recorded. The efficiency of the control strategy among HCWs was evaluated identifying symptomatic infection (primary endpoint) and asymptomatic infection (secondary endpoint) with confirmed detection of SARS-CoV-2. Results: 7595 patients were evaluated in the Advanced Triage area: 5.2% resulted positive and 72.4% was symptomatic. The HCW team was composed of 60 members. A total of 361 nasopharyngeal swabs were performed on HCWs. All the swabs resulted negative and none of the HCWs reached the primary or the secondary endpoint. Conclusions: An integrated hospital infection control strategy, consisting of dedicated areas for infected patients, strict measures for PPE use and mass surveillance, is successful to prevent infection among HCWs.
The Role of the Liver-Specific microRNA, miRNA-122 in the HCV Replication Cycle
Authors: Kunden Rasika D. , Khan Juveriya Q. , Ghezelbash Sarah , Wilson Joyce A.
Published: August 2020

Hepatitis C virus (HCV) replication requires annealing of a liver specific microRNA, miR-122 to 2 sites on 5′ untranslated region (UTR). While, microRNAs downregulate gene expression by binding to the 3′ untranslated region of the target mRNA, in this case, the microRNA anneals to the 5′UTR of the viral genomes and upregulates the viral lifecycle. In this review, we explore the current understandings of the mechanisms by which miR-122 promotes the HCV lifecycle, and its contributions to pathogenesis. Annealing of miR-122 has been reported to (a) stimulate virus translation by promoting the formation of translationally active internal ribosome entry site (IRES) RNA structure, (b) stabilize the genome, and (c) induce viral genomic RNA replication. MiR-122 modulates lipid metabolism and suppresses tumor formation, and sequestration by HCV may influence virus pathogenesis. We also discuss the possible use of miR-122 as a biomarker for chronic hepatitis and as a therapeutic target. Finally, we discuss roles for miR-122 and other microRNAs in promoting other viruses.
Aberrant CXCR4 Signaling at Crossroad of WHIM Syndrome and Waldenstrom’s Macroglobulinemia
Authors: Milanesi Samantha , Locati Massimo , Borroni Elena Monica
Published: August 2020

Given its pleiotropic functions, including its prominent role in inflammation, immune responses and cancer, the C-X-C chemokine receptor type 4 (CXCR4) has gained significant attention in recent years and has become a relevant target in drug development. Although the signaling properties of CXCR4 have been extensively studied, several aspects deserve deeper investigations. Mutations in the C-term tail of the CXCR4 gene cause WHIM syndrome, a rare congenital immunodeficiency associated by chronic leukopenia. Similar mutations have also been recently identified in 30% of patients affected by Waldenstrom’s macroglobulinaemia, a B-cell neoplasia with bone marrow accumulation of malignant cells. An ample body of work has been generated to define the impact of WHIM mutations on CXCR4 signaling properties and evaluate their role on pathogenesis, diagnosis, and response to therapy, although the identity of disease-causing signaling pathways and their relevance for disease development in different genetic variants are still open questions. This review discusses the current knowledge on biochemical properties of CXCR4 mutations to identify their prototypic signaling profile potentially useful to highlighting novel opportunities for therapeutic intervention.
Systematic Review—The Potential Implications of Different Platelet-Rich Plasma (PRP) Concentrations in Regenerative Medicine for Tissue Repair
Authors: Gentile Pietro , Garcovich Simone
Published: August 2020

The number of studies evaluating platelet-rich plasma (PRP) concentration has substantially grown in the last fifteen years. A systematic review on this field has been realized by evaluating in the identified studies the in vitro PRP concentration—also analyzing the platelet amount—and the in vivo PRP effects in tissue regeneration compared to any control. The protocol has been developed in agreement with the Preferred Reporting for Items for Systematic Reviews and Meta-Analyses-Protocols (PRISMA-P) guidelines. Multistep research of the PubMed, MEDLINE, Embase, PreMEDLINE, Ebase, CINAHL, PsycINFO, Clinicaltrials.gov, Scopus database and Cochrane databases has permitted to identify articles on different concentrations of PRP in vitro and related in vivo impact for tissue repair. Of the 965 articles initially identified, 30 articles focusing on PRP concentration have been selected and, consequently, only 15 articles have been analyzed. In total, 40% (n = 6) of the studies were related to the fixed PRP Concentration Group used a fixed PRP concentration and altered the platelet concentration by adding the different volumes of the PRP (lysate) to the culture. This technique led to a substantial decrease in nutrition available at higher concentrations. Sixty percent (n = 9) of the studies were related to the fixed PRP Volume Group that used a fixed PRP-to-media ratio (Vol/Vol) throughout the experiment and altered the concentration within the PRP volume. For both groups, when the volume of medium (nutrition) decreases, a lower rate of cell proliferation is observed. A PRP concentration of 1.0 × 106 plt/μL, appears to be optimal thanks to the constant and plentiful capillary nutrition supply and rapid diffusion of growth factors that happen in vivo and it also respects the blood decree-law. The PRP/media ratio should provide a sufficient nutrition supply to prevent cellular starvation, that is, PRP ≤ 10% (Vol/Vol) and thus best mimic the conditions in vivo.
DeepPred-SubMito: A Novel Submitochondrial Localization Predictor Based on Multi-Channel Convolutional Neural Network and Dataset Balancing Treatment
Authors: Wang Xiao , Jin Yinping , Zhang Qiuwen
Published: August 2020

Mitochondrial proteins are physiologically active in different compartments, and their abnormal location will trigger the pathogenesis of human mitochondrial pathologies. Correctly identifying submitochondrial locations can provide information for disease pathogenesis and drug design. A mitochondrion has four submitochondrial compartments, the matrix, the outer membrane, the inner membrane, and the intermembrane space, but various existing studies ignored the intermembrane space. The majority of researchers used traditional machine learning methods for predicting mitochondrial protein localization. Those predictors required expert-level knowledge of biology to be encoded as features rather than allowing the underlying predictor to extract features through a data-driven procedure. Besides, few researchers have considered the imbalance in datasets. In this paper, we propose a novel end-to-end predictor employing deep neural networks, DeepPred-SubMito, for protein submitochondrial location prediction. First, we utilize random over-sampling to decrease the influence caused by unbalanced datasets. Next, we train a multi-channel bilayer convolutional neural network for multiple subsequences to learn high-level features. Third, the prediction result is outputted through the fully connected layer. The performance of the predictor is measured by 10-fold cross-validation and 5-fold cross-validation on the SM424-18 dataset and the SubMitoPred dataset, respectively. Experimental results show that the predictor outperforms state-of-the-art predictors. In addition, the prediction of results in the M983 dataset also confirmed its effectiveness in predicting submitochondrial locations.
Erratum: Willenbacher, E., et al. Curcumin: New Insights into an Ancient Ingredient against Cancer. Int. J. Mol. Sci. 2019, 20, 1808
Authors: Willenbacher Ella , Khan Shah Zeb , Mujica Sara Cecilia Altuna , Trapani Dario , Hussain Sadaqat , Wolf Dominik , Willenbacher Wolfgang , Spizzo Gilbert , Seeber Andreas
Published: August 2020

We would like to change the authors’ names and affiliations on Page 1 of paper [...]
Lithium Chloride Sensitivity in Yeast and Regulation of Translation
Authors: Hajikarimlou Maryam , Hunt Kathryn , Kirby Grace , Takallou Sarah , Jagadeesan Sasi Kumar , Omidi Katayoun , Hooshyar Mohsen , Burnside Daniel , Moteshareie Houman , Babu Mohan , Smith Myron , Holcik Martin , Samanfar Bahram , Golshani Ashkan
Published: August 2020

For decades, lithium chloride (LiCl) has been used as a treatment option for those living with bipolar disorder (BD). As a result, many studies have been conducted to examine its mode of action, toxicity, and downstream cellular responses. We know that LiCl is able to affect cell signaling and signaling transduction pathways through protein kinase C and glycogen synthase kinase-3, which are considered to be important in regulating gene expression at the translational level. However, additional downstream effects require further investigation, especially in translation pathway. In yeast, LiCl treatment affects the expression, and thus the activity, of PGM2, a phosphoglucomutase involved in sugar metabolism. Inhibition of PGM2 leads to the accumulation of intermediate metabolites of galactose metabolism causing cell toxicity. However, it is not fully understood how LiCl affects gene expression in this matter. In this study, we identified three genes, NAM7, PUS2, and RPL27B, which increase yeast LiCl sensitivity when deleted. We further demonstrate that NAM7, PUS2, and RPL27B influence translation and exert their activity through the 5′-Untranslated region (5′-UTR) of PGM2 mRNA in yeast.
Lysosomal Storage Disease-Associated Neuropathy: Targeting Stable Nucleic Acid Lipid Particle (SNALP)-Formulated siRNAs to the Brain as a Therapeutic Approach
Authors: Coutinho Maria Francisca , Santos Juliana Inês , S. Mendonça Liliana , Matos Liliana , Prata Maria João , S. Jurado Amália , Pedroso de Lima Maria C. , Alves Sandra
Published: August 2020

More than two thirds of Lysosomal Storage Diseases (LSDs) present central nervous system involvement. Nevertheless, only one of the currently approved therapies has an impact on neuropathology. Therefore, alternative approaches are under development, either addressing the underlying enzymatic defect or its downstream consequences. Also under study is the possibility to block substrate accumulation upstream, by promoting a decrease of its synthesis. This concept is known as substrate reduction therapy and may be triggered by several molecules, such as small interfering RNAs (siRNAs). siRNAs promote RNA interference, a naturally occurring sequence-specific post-transcriptional gene-silencing mechanism, and may target virtually any gene of interest, inhibiting its expression. Still, naked siRNAs have limited cellular uptake, low biological stability, and unfavorable pharmacokinetics. Thus, their translation into clinics requires proper delivery methods. One promising platform is a special class of liposomes called stable nucleic acid lipid particles (SNALPs), which are characterized by high cargo encapsulation efficiency and may be engineered to promote targeted delivery to specific receptors. Here, we review the concept of SNALPs, presenting a series of examples on their efficacy as siRNA nanodelivery systems. By doing so, we hope to unveil the therapeutic potential of these nanosystems for targeted brain delivery of siRNAs in LSDs.
Structural Features of 1,3,4-Thiadiazole-Derived Ligands and Their Zn(II) and Cu(II) Complexes Which Demonstrate Synergistic Antibacterial Effects with Kanamycin
Authors: Karcz Dariusz , Matwijczuk Arkadiusz , Kamiński Daniel , Creaven Bernadette , Ciszkowicz Ewa , Lecka-Szlachta Katarzyna , Starzak Karolina
Published: August 2020

Classical synthetic protocols were applied for the isolation of three novel 1,3,4-thiadiazole derivatives which were then complexed with the biologically important Cu(II) and Zn(II) ions. All free ligands and their corresponding complexes were characterized using a number of spectroscopic techniques including Ultraviolet-visible (UV–vis), Fluorescence, Infrared (FT-IR), tandem liquid chromatography-mass (LC-MS), X-ray diffraction (XRD), and Nuclear Magnetic Resonance (NMR) spectroscopy (1H, 13C, HSQC, HMBC). The results obtained are consistent with the formation of dihydrate complexes, in which the chelation of the metal ion occurs via one of the thiadiazole nitrogen atoms and the deprotonated hydroxyl group of the neighboring resorcynyl moiety. The Zn(II) complexes utilize a 1:1 ligand–metal ratio, while in the Cu(II) complexes the ligand–metal ratio is 2:1. Although the antibacterial testing identified moderate activity of the compounds against the tested bacterial strains and additionally modest antioxidant activity, a strong synergistic antibacterial effect against Staphylococcus aureus, using concomitant treatment of thiadiazole derivatives with the commercial antibiotic kanamycin, was observed. The most active thiadiazole derivative demonstrated a minimal inhibitory concentration (MIC) of 500 μg/mL while it was 125 μg/mL in the presence of kanamycin. Moreover, in the presence of few thiadiazole derivatives the MIC value of kanamycin decreased from 0.39 μg/mL to 0.5 μg/mL. The antioxidant activity (IC50) of the most active thiadiazole derivative was determined as 0.13 mM which was nearly three-fold lower compared to that of TROLOX (0.5 mM).
Extrahepatic Drug Transporters in Liver Failure: Focus on Kidney and Gastrointestinal Tract
Authors: Droździk Marek , Oswald Stefan , Droździk Agnieszka
Published: August 2020

Emerging information suggests that liver pathological states may affect the expression and function of membrane transporters in the gastrointestinal tract and the kidney. Altered status of the transporters could affect drug as well as endogenous compounds handling with subsequent clinical consequences. It seems that changes in intestinal and kidney transporter functions provide the compensatory activity of eliminating endogenous compounds (e.g., bile acids) generated and accumulated due to liver dysfunction. A literature search was conducted on the Ovid and PubMed databases to select relevant in vitro, animal and human studies that have reported expression, protein abundance and function of the gastrointestinal and kidney operating ABC (ATP-binding cassette) transporters and SLC (solute carriers) carriers. The accumulated data suggest that liver failure-associated transporter alterations in the gastrointestinal tract and kidney may affect drug pharmacokinetics. The altered status of drug transporters in those organs in liver dysfunction conditions may provide compensatory activity in handling endogenous compounds, affecting local drug actions as well as drug pharmacokinetics.
Development of a Method to Study Evaporation of a Volatile Solvent in an Isolated Subsurface Structure: A Practical Exercise in Risk Minimization
Authors: McManus Thomas Neil , Rosa Ana , Haddad Assed
Published: August 2020

This article describes development and confirmatory testing of a method to study the evaporation of a volatile solvent containing ignitable ingredients in an isolated subsurface structure, a type of confined space. Accidental spillage and surreptitious disposal of chemical products in streets create a risk of fire and explosions in these structures. Development of the method included consideration about instrument safety; personal exposure; volume of the structure (2.5 m3); evaporation rate; temperature of the airspace; and number of opening(s) in the manhole cover. Confirmatory testing utilized 10 mL of lacquer thinner (60% to 80% toluene, 10% to 20% methylethyl ketone (MEK), 5% to 10% methanol and 1% to 9% acetone) on a wetted paper towel positioned near the bottom of the structure. This methodology produced a maximum of 2150 ppm of ‘isobutylene units’ on a PID (PhotoIonization sensor) positioned about 15 cm above the sample. This concentration corresponds to about 1140 ppm of toluene (less than 10% of the Lower Flammable Limit of 12,700 ppm). This method offers a stable, safe platform for study of the process. Evaporation of solvent and exchange between the external atmosphere and the airspace regulate the concentration of vapor, which can typically persist for 24 to 48 h.
The Inability of Spotted Lanternfly (Lycorma delicatula) to Vector a Plant Pathogen between its Preferred Host, Ailanthus altissima, in a Laboratory Setting
Authors: Brooks Rachel K. , Toland Ashley , Dechaine Andrew C. , McAvoy Thomas , Salom Scott
Published: August 2020

With the recent introduction of the non-native spotted lanternfly (Lycorma delicatula) to the USA, research and concern regarding this insect is increasing. Though L. delicatula is able to feed on many different plant species, its preference for the invasive tree-of-heaven (Ailanthus altissima) is apparent, especially during its later life stage. Therefore, management focused on A. altissima control to help limit L. delicatula establishment and population growth has become popular. Unfortunately, the control of A. altissima is difficult. Verticillium nonalfalfae, a naturally occurring vascular-wilt pathogen, has recently received attention as a potential biological control agent. Therefore, we studied if L. delicatula fourth instars or adults could vector V. nonalfalfae from infected A. altissima material to healthy A. altissima seedlings in a laboratory setting. We were unable to re-isolate V. nonalfalfae from the 45 A. altissima seedlings or from the 225 L. delicatula utilized in this experiment. We therefore, found no support that L. delicatula could effectively vector this pathogen between A. altissima in laboratory conditions. Since L.delicatula’s ability to vector V. nonalfalfae has implications for the dissemination of both this beneficial biological control and other similar unwanted plant pathogens, future research is needed to confirm these findings in a field setting.
Liquid Biopsy: A Biomarker-Driven Tool towards Precision Oncology
Authors: Yee Nelson S.
Published: August 2020

Liquid biopsy or the sampling of bodily fluids, mostly blood, has been intensely investigated and developed for clinical utility in medicine, especially oncology [...]
Knowledge on Signs and Risk Factors in Stroke Patients
Authors: Soto-Cámara Raúl , González-Bernal Jerónimo J. , González-Santos Josefa , Aguilar-Parra José M. , Trigueros Rubén , López-Liria Remedios
Published: August 2020

Background: There is a pressing need to contribute evidence to the improvement in the early identification of signs and symptoms associated with strokes, and address the treatment-seeking delays. The objective of this study is to describe the knowledge regarding the warning signs and risk factors (RFs) among stroke patients, as well as of their attitudes toward a suspected event, and the analysis of its possible relationship with the socio-demographic and clinical characteristics of these patients. Method: A cross-sectional study was designed, in which all stroke patients admitted consecutively to the Burgos University Hospital (Spain) were included. The principal outcomes were the patient’s ability to identify two RFs and two warning signs and the patient’s hypothetical response to a possible stroke event. The possible factors associated with the knowledge of warning signs, RFs, and the correct response to a new event were studied using univariate and multivariate regression analysis. Results: A total of 529 patients were included. Having a higher education level or a history of prior stroke were associated with a greater degree of knowledge of warning signs (odds ratio (OR) 3.19, 95% confidence interval (CI) 1.70–5.74, p = 0.003; OR 3.54, 95%CI 2.09–5.99, p ≤ 0.001, respectively), RFs (OR 3.15, 95%CI 1.75–5.67, p = 0.008; OR 4.08, 95%CI 2.41–6.91, p = 0.002, respectively), and the correct response to a possible stroke (OR 1.82, 95%CI 1.16–2.86; p = 0.030; OR 2.11, 95%CI 1.29–3.46, p = 0.022, respectively). Conclusion: Knowledge of warning signs or stroke RFs is low in the hospitalized patients. A previous stroke or secondary/higher education levels are the predictor factors that increase the probability of knowledge of warning signs, RFs, or reaction to possible event.
Three Thousand Consecutive Pancreaticoduodenectomies in a Tertiary Cancer Center: A Retrospective Observational Study
Authors: Jung Ji Hye , Choi Dong Wook , Yoon Sokyung , Yoon So Jeong , Han In Woong , Heo Jin Seok , Shin Sang Hyun
Published: August 2020

(1) Aim: To evaluate clinicopathological features and postoperative outcomes including survival in patients who underwent pancreaticoduodenectomy (PD) for periampullary diseases. (2) Methods: We retrospectively reviewed 3078 cases of PD performed in our center for 25 years. Periampullary diseases were divided into benign and malignancy groups. All cases were also classified by location. The time of 25 years was divided to different periods (5 years per period) to compare outcomes. Overall survival was compared between subdivided periods. (3) Results: Hospitalization became significantly shorter from 28.0 days in the 1st period to 13.8 days in the 5th period. Overall complication rate was significantly increased since the 3rd period. The rate without postoperative pancreatic fistula (POPF) was high at 98.7% in the 1st period. This might be because drain amylase on the 3rd day after PD was not routinely checked in the past. Thus, POPF was not detected. In survival analysis of adenocarcinoma of pancreas, bile duct, and ampulla, overall survival was found to be improved significantly in recent years. (4) Conclusions: Our study revealed that outcomes were improved with increasing number of PDs performed. Although POPF and overall complications showed increases more recently, those were detected and managed, resulting in shorter hospitalization and improved outcomes.
Efficacy, Safety, and Tolerability of Treatments for Systemic Sclerosis-Related Interstitial Lung Disease: A Systematic Review and Network Meta-Analysis
Authors: Erre Gian Luca , Sebastiani Marco , Fenu Maria Antonietta , Zinellu Angelo , Floris Alberto , Cavagna Lorenzo , Renzoni Elisabetta , Manfredi Andreina , Passiu Giuseppe , Woodman Richard John , Mangoni Arduino Aleksander
Published: August 2020

Background: There is a paucity of head-to-head comparisons of the efficacy and harms of pharmacological treatments for systemic sclerosis-related interstitial lung disease (SSc-ILD). Methods: We conducted a network meta-analysis (NMA) in order to compare the effects of different treatments with the placebo on change in forced vital capacity (FVC), change in diffusion lung capacity for CO (DLCO), serious adverse events (SAEs), discontinuation for adverse events and mortality in SSc-ILD. Standardized mean difference (SMD) and log odds ratio were estimated using NMA with fixed effects. Results: Nine randomized clinical trials (926 participants) comparing eight interventions and the placebo for an average follow-up of one year were included. Compared to the placebo, only rituximab significantly reduced FVC decline (SMD (95% CI) = 1.00 (0.39 to 1.61)). Suitable data on FVC outcome for nintedanib were not available for the analysis. No treatments influenced DLCO. Safety and mortality were also not different across treatments and the placebo, although there were few reported events. Cyclophosphamide and pomalidomide were less tolerated than the placebo, mycophenolate, and nintedanib. Conclusion: Only rituximab significantly reduced lung function decline compared to the placebo. However, direct head-to-head comparison studies are required to confirm these findings and to better determine the safety profile of various treatments.
The War against Bad Bugs: Fighting the Resistance
Authors: Ceccato Adrian , Torres Antoni
Published: August 2020

Multidrug-resistant (MDR) microorganisms have become a growing concern, especially in regions with high prevalence [...]
The Impact of Spinopelvic Mobility on Arthroplasty: Implications for Hip and Spine Surgeons
Authors: Haffer Henryk , Adl Amini Dominik , Perka Carsten , Pumberger Matthias
Published: August 2020

Spinopelvic mobility represents the complex interaction of hip, pelvis, and spine. Understanding this interaction is relevant for both arthroplasty and spine surgeons, as a predicted increasing number of patients will suffer from hip and spinal pathologies simultaneously. We conducted a comprehensive literature review, defined the nomenclature, summarized the various classifications of spinopelvic mobility, and outlined the corresponding treatment algorithms. In addition, we developed a step-by-step workup for spinopelvic mobility and total hip arthroplasty (THA). Normal spinopelvic mobility changes from standing to sitting; the hip flexes, and the posterior pelvic tilt increases with a concomitant increase in acetabular anteversion and decreasing lumbar lordosis. Most classifications are based on a division of spinopelvic mobility based on ΔSS (sacral slope) into stiff, normal, and hypermobile, and a categorization of the sagittal spinal balance regarding pelvic incidence (PI) and lumbar lordosis (LL) mismatch (PI–LL = ± 10° balanced versus PI–LL > 10° unbalanced) and corresponding adjustment of the acetabular component implantation. When performing THA, patients with suspected pathologic spinopelvic mobility should be identified by medical history and examination, and a radiological evaluation (a.p. pelvis standing and lateral femur to L1 or C7 (if EOS (EOS imaging, Paris, France) is available), respectively, for standing and sitting radiographs) of spinopelvic parameters should be conducted in order to classify the patient and determine the appropriate treatment strategy. Spine surgeons, before planned spinal fusion in the presence of osteoarthritis of the hip, should consider a hip flexion contracture and inform the patient of an increased risk of complications with existing or planned THA.
Promises of Fibroblast Growth Factor Receptor–Directed Therapy in Tailored Cancer Treatment
Authors: Fagoonee Sharmila , Pellicano Rinaldo
Published: August 2020

Cancer, one of the deadliest and undefeatable diseases, involves the deregulated growth of cells with the conferment of a high potential to metastasize [...]
Molecular Mechanisms of AKI in the Elderly: From Animal Models to Therapeutic Intervention
Authors: Infante Barbara , Franzin Rossana , Madio Desirèe , Calvaruso Martina , Maiorano Annamaria , Sangregorio Fabio , Netti Giuseppe Stefano , Ranieri Elena , Gesualdo Loreto , Castellano Giuseppe , Stallone Giovanni
Published: August 2020

Acute kidney injury (AKI), a critical syndrome characterized by a sudden reduction of renal function, is a common disorder among elderly patients particularly in Intensive Care Unit (ICU). AKI is closely associated with both short- and long-term mortality and length of hospital stay and is considered a predictor of chronic kidney disease (CKD). Specific hemodynamic, metabolic, and molecular changes lead to increased susceptibility to injury in the aged kidney; therefore, certain causes of AKI such as the prerenal reduction in renal perfusion or vascular obstructive conditions are more common in the elderly; moreover, AKI is often multifactorial and iatrogenic. Older patients present several comorbidities (diabetes, hypertension, heart failure) and are exposed to multiple medical interventions such as the use of nephrotoxic contrasts media and medications, which can also trigger AKI. Considering the emerging relevance of this condition, prevention and treatment of AKI in the elderly should be crucial in the internist and emergency setting. This review article summarizes the incidence, the risk factors, the pathophysiology, the molecular mechanisms and the strategies of prevention and treatment of AKI in elderly patients.
Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia
Authors: Mietus Constance J. , Lackner Timothy J. , Karvelis Petros S. , Willcockson Gregory T. , Shields Christina M. , Lambert Nicholas G. , Koutakis Panagiotis , Fuglestad Matthew A. , Hernandez Hernan , Haynatzki Gleb R. , Kim Julian K. S. , DeSpiegelaere Holly K. , Pipinos Iraklis I. , Casale George P.
Published: August 2020

Work from our laboratory documents pathological events, including myofiber oxidative damage and degeneration, myofibrosis, micro-vessel (diameter = 50–150 μm) remodeling, and collagenous investment of terminal micro-vessels (diameter ≤ 15 µm) in the calf muscle of patients with Peripheral Artery Disease (PAD). In this study, we evaluate the hypothesis that the vascular pathology associated with the legs of PAD patients encompasses pathologic changes to the smallest micro-vessels in calf muscle. Biopsies were collected from the calf muscle of control subjects and patients with Fontaine Stage II and Stage IV PAD. Slide specimens were evaluated by Quantitative Multi-Spectral and Fluorescence Microscopy. Inter-myofiber collagen, stained with Masson Trichrome (MT), was increased in Stage II patients, and more substantially in Stage IV patients in association with collagenous thickening of terminal micro-vessel walls. Evaluation of the Basement Membrane (BM) of these vessels reveals increased thickness in Stage II patients, and increased thickness, diameter, and Collagen I deposition in Stage IV patients. Coverage of these micro-vessels with pericytes, key contributors to fibrosis and BM remodeling, was increased in Stage II patients, and was greatest in Stage IV patients. Vascular pathology of the legs of PAD patients extends beyond atherosclerotic main inflow arteries and affects the entire vascular tree—including the smallest micro-vessels.
Soft Tissue Dimensions Following Tooth Extraction in the Posterior Maxilla: A Randomized Clinical Trial Comparing Alveolar Ridge Preservation to Spontaneous Healing
Authors: Song Young Woo , Yoon Sung-Wook , Cha Jae-Kook , Jung Ui-Won , Jung Ronald E. , Thoma Daniel S.
Published: August 2020

Background: To assess the soft tissue dimension following tooth extraction and alveolar ridge preservation in the posterior maxilla compared to spontaneous healing. Methods: Thirty-five patients randomly assigned to alveolar ridge preservation (ARP) and spontaneous healing (SH) after maxillary molar extraction. The crestal, buccal, and palatal gingival thickness at 6 months was measured around virtually placed implant fixtures using superimposed cone-beam computed tomography and intraoral scan taken at 6 months. Buccal mucogingival junction (MGJ) level change over 6 months was estimated using intraoral scans obtained at suture-removal and 6 months. Results: The crestal gingiva was significantly thinner in group ARP (−1.16 mm) compared to group SH (p < 0.05). The buccal and palatal gingiva was significantly thinner at the implant shoulder (IS) level in group ARP (buccal: −0.75 mm; palatal: −0.85 mm) compared to group SH (p < 0.05). The thickness at 2 mm below the IS of both sides and the buccal MGJ level change were similar in both groups (p > 0.05). Conclusions: ARP in the posterior maxilla resulted in a thinner soft tissue on top of and at the prospective level of the implant shoulder at 6 months. The buccal MGJ level changed minimal for 6 months in both groups.
(Neuro) Peptides, Physical Activity, and Cognition
Authors: Autio Juho , Stenbäck Ville , Gagnon Dominique D. , Leppäluoto Juhani , Herzig Karl-Heinz
Published: August 2020

Regular physical activity (PA) improves cognitive functions, prevents brain atrophy, and delays the onset of cognitive decline, dementia, and Alzheimer’s disease. Presently, there are no specific recommendations for PA producing positive effects on brain health and little is known on its mediators. PA affects production and release of several peptides secreted from peripheral and central tissues, targeting receptors located in the central nervous system (CNS). This review will provide a summary of the current knowledge on the association between PA and cognition with a focus on the role of (neuro)peptides. For the review we define peptides as molecules with less than 100 amino acids and exclude myokines. Tachykinins, somatostatin, and opioid peptides were excluded from this review since they were not affected by PA. There is evidence suggesting that PA increases peripheral insulin growth factor 1 (IGF-1) levels and elevated serum IGF-1 levels are associated with improved cognitive performance. It is therefore likely that IGF-1 plays a role in PA induced improvement of cognition. Other neuropeptides such as neuropeptide Y (NPY), ghrelin, galanin, and vasoactive intestinal peptide (VIP) could mediate the beneficial effects of PA on cognition, but the current literature regarding these (neuro)peptides is limited.
A Comparative Study on Cure Kinetics of Layered Double Hydroxide (LDH)/Epoxy Nanocomposites
Authors: Karami Zohre , Paran Seyed Mohammad Reza , Vijayan P. Poornima , Ganjali Mohammad Reza , Jouyandeh Maryam , Esmaeili Amin , Habibzadeh Sajjad , J. Stadler Florian , Saeb Mohammad Reza
Published: August 2020

Layered double hydroxide (LDH) minerals are promising candidates for developing polymer nanocomposites and the exchange of intercalating anions and metal ions in the LDH structure considerably affects their ultimate properties. Despite the fact that the synthesis of various kinds of LDHs has been the subject of numerous studies, the cure kinetics of LDH-based thermoset polymer composites has rarely been investigated. Herein, binary and ternary structures, including [Mg0.75 Al0.25 (OH)2]0.25+ [(CO32−)0.25/2∙m H2O]0.25−, [Mg0.75 Al0.25 (OH)2]0.25+ [(NO3−)0.25∙m H2O]0.25− and [Mg0.64 Zn0.11 Al0.25 (OH)2]0.25+ [(CO32−)0.25/2∙m H2O]0.25−, have been incorporated into epoxy to study the cure kinetics of the resulting nanocomposites by differential scanning calorimetry (DSC). Both integral and differential isoconversional methods serve to study the non-isothermal curing reactions of epoxy nanocomposites. The effects of carbonate and nitrate ions as intercalating agents on the cure kinetics are also discussed. The activation energy of cure (Eα) was calculated based on the Friedman and Kissinger–Akahira–Sunose (KAS) methods for epoxy/LDH nanocomposites. The order of autocatalytic reaction (m) for the epoxy/Mg-Al-NO3 (0.30 and 0.254 calculated by the Friedman and KAS methods, respectively) was smaller than that of the neat epoxy, which suggested a shift of the curing mechanism from an autocatalytic to noncatalytic reaction. Moreover, a higher frequency factor for the aforementioned nanocomposite suggests that the incorporation of Mg-Al-NO3 in the epoxy composite improved the curability of the epoxy. The results elucidate that the intercalating anions and the metal constituent of LDH significantly govern the cure kinetics of epoxy by the participation of nitrate anions in the epoxide ring-opening reaction.
UAV Trajectory Planning in a Port Environment
Authors: Tang Gang , Hou Zhipeng , Claramunt Christophe , Hu Xiong
Published: August 2020

In many situations, the trajectory of an unmanned aerial vehicle (UAV) is very likely to deviate from the initial path generated by a path planning algorithm. This is in fact due to the existence of dynamic constraints of the UAV. In order to reduce the degree of such a deviation, this research introduces a trajectory planning algorithm, the objective of which is to minimize distance while maintaining security. The algorithm first develops preprocess trajectory points by constructing isosceles triangles then, on the basis of a minimum snap trajectory method, it applies a corridor constraint to an optimization objective function, while the deviation evaluation function is established to quantitatively evaluate the deviation distance. A series of experiments were carried out in a simulation environment with a simplified quay crane model. The results show that the proposed method not only optimizes the time and length of the generated trajectory, but also reduces the average deviation distance by 88.7%. Moreover, the generated trajectory can be well tracked by the UAV through qualitative and quantitative analysis. Overall, the experiments show that the proposed method can generate a higher UAV trajectory quality.
Antimicrobial Activities of Sponge-Derived Microorganisms from Coastal Waters of Central Vietnam
Authors: Mien Pham Thi , Ha Dao Viet , Ben Hoang Xuan , Chen Bin , Liu Lan , Minh-Thu Phan
Published: August 2020

Bacteria associated with invertebrates are considered as good sources for biologically active compounds. Sponge-derived bacteria were screened for antimicrobial activities, the presence of the cluster genes of polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs), and through a colony picking method. Crude extracts of broth cultures were tested for microbial inhibition. Eleven out of 25 isolated strains showed inhibition of at least one of eight tested indicator microorganisms. Antimicrobial activities were observed in the strains coded HM5, HM6, and HM9 with the presence of NRPS and PKSII genes, whereas the isolate HM21 held both NRPS and PKSII and inhibited only the growth of Bacillus subtilis by the well diffusion method and only inhibited Serratia marcescens by the colony picking method. Two isolates, HM5 and HM6, belonged to the species of Bacillus. Interestingly, the isolate HM9 was nearest to Streptomyces mexicanusT NBRC100915 (GenBank accession number AB249966) with 94% sequence similarity. This potent strain HM9 could possibly be considered as a new species and a good source for bioactive compound discovery. Some isolates showed NRPS/PKS genes but did not exhibit antimicrobial activity. Thus, we suggested that both molecular and traditional methods should be conducted for the screening of antimicrobial producers.
The Effect of Exchange Rate Volatility on Economic Growth: Case of the CEE Countries
Authors: Morina Fatbardha , Hysa Eglantina , Ergün Uğur , Panait Mirela , Voica Marian Catalin
Published: August 2020

The exchange rate is a key macroeconomic factor that affects international trade and the real economy of each country. The development of international trade creates conditions where volatility comes with the exchange rate. The purpose of this paper is to examine the effect of real effective exchange rate volatility on economic growth in the Central and Eastern European countries. Additionally, the effect, through three channels of influence on economic growth which vary on the measurement of exchange rate volatility, is examined. The study uses annual data for fourteen CEE countries for the period 2002–2018 to examine the nature and extends the impact of such movements on growth. The empirical findings using the fixed effects estimation for panel data reveal that the volatility of the exchange rate has a significant negative effect on real economic growth. The results appear robust with alternative measures of exchange rate volatility such as standard deviation and z-score. This paper suggests that policymakers should adopt different policies to keep the exchange rate stable in order to foster economic growth.
Diversification of Livestock-Keeping Smallholders in Mountainous Rural Regions of Azerbaijan and Georgia
Authors: Neudert Regina , Allahverdiyeva Naiba , Mammadov Niyaz , Didebulidze Alexandre , Beckmann Volker
Published: August 2020

Diversified livelihoods combining farming, livestock keeping and non-farm income are characteristic of many rural households worldwide. For the Central Asian and Caucasian region, livestock keeping is especially important in terms of land use and socio-cultural heritage. We contribute to the literature with data from the under-researched Caucasus region and investigate: (i) the extent of diversification in smallholder households; (ii) the role of livestock keeping in diversification; (iii) the influence of household-specific and location-specific variables and diversification on household income. Based on a dataset of 303 households, we calculate contribution margins for the main agricultural activities, household income, and diversification indices and analyze the influence of diversification, asset and location variables on household income with a regression model. Household income is generally diversified and a combination of four income sources (crops, livestock, poultry/bees and social benefits) was the most frequent. The econometric analysis shows that higher household incomes are positively correlated with higher household land and livestock assets, the presence of non-farm work and social benefit income sources and with an increasing specialization as measured by the diversification index. For enhancing rural household incomes and slowing down rural-urban migration, the development of non-farm job opportunities is recommended.