bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of Aroma-Active Compounds in Seed Extract of Black Cumin (Nigella sativa L.) by Aroma Extract Dilution Analysis
Authors: Kesen Songul , Amanpour Armin , Tsouli Sarhir Salwa , Sevindik Onur , Guclu Gamze , Kelebek Hasim , Selli Serkan
Published: June 2018

Turkish Nigella sativa L. seed extracts were used to detect the aroma and key odorant compounds of the spice using gas chromatography-mass spectrometry-olfactometry (GC-MS-O). Volatile compounds were extracted by the purge and trap extraction (PTE) method. A total of 32 volatile compounds consisting of different chemical classes acids (13), alcohols (7), phenols (3), terpene (1), esters (2), ketones (2), aldehyde (1), lactone (1) and hydrocarbons (2) were determined. The amounts of volatile compounds were found to be 21,544 µg kg−1. The application of aroma extract dilution analysis (AEDA) revealed the presence of 13 odor-active compounds alcohols (2), carboxylic acids (4), phenols (2), terpene (1), ketone (1), hydrocarbon (1) and unknown compounds (2) in Nigella sativa L. extract. Flavor dilution (FD) factors of key odorants ranged between 4 and 1024, while odor activity values (OAV) were in the range of 1.0 to 170.8. Acetoin was the only aroma-active ketone detected in Nigella sativa L. seed extracts. It had the strongest aroma (FD = 1024) and provided a buttery odor. This compound represented the most abundant compound of overall aroma profile with a concentration of 9394 µg kg−1, followed by isobutanoic acid (FD = 512 with a concentration of 218 µg kg−1) and contributed a powerful aroma and a cheesy characteristic odor.
Comparative Transcriptome Analysis Identifies Genes Putatively Involved in 20-Hydroxyecdysone Biosynthesis in Cyanotis arachnoidea
Authors: Lei Xiu , Xia Jing , Wang Jian , Zheng Li
Published: June 2018

Cyanotis arachnoidea contains a rich array of phytoecdysteroids, including 20-hydroxyecdysone (20E), which displays important agrochemical, medicinal, and pharmacological effects. To date, the biosynthetic pathway of 20E, especially the downstream pathway, remains largely unknown. To identify candidate genes involved in 20E biosynthesis, the comparative transcriptome of C. arachnoidea leaf and root was constructed. In total, 86.5 million clean reads were obtained and assembled into 79,835 unigenes, of which 39,425 unigenes were successfully annotated. The expression levels of 2427 unigenes were up-regualted in roots with a higher accumulation of 20E. Further assignments with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways identified 49 unigenes referring to the phytoecdysteroid backbone biosynthesis (including 15 mevalonate pathway genes, 15 non-mevalonate pathway genes, and 19 genes for the biosynthesis from farnesyl pyrophosphate to cholesterol). Moreover, higher expression levels of mevalonate pathway genes in roots of C. arachniodea were confirmed by real-time quantitative PCR. Twenty unigenes encoding CYP450s were identified to be new candidate genes for the bioreaction from cholesterol to 20E. In addition, 90 transcription factors highly expressed in the roots and 15,315 unigenes containing 19,158 simple sequence repeats (SSRs) were identified. The transcriptome data of our study provides a valuable resource for the understanding of 20E biosynthesis in C. arachnoidea.
Preliminary RNA-Seq Analysis of Long Non-Coding RNAs Expressed in Human Term Placenta
Authors: Majewska Marta , Lipka Aleksandra , Paukszto Lukasz , Jastrzebski Jan Pawel , Gowkielewicz Marek , Jozwik Marcin , Majewski Mariusz Krzysztof
Published: June 2018

Development of particular structures and proper functioning of the placenta are under the influence of sophisticated pathways, controlled by the expression of substantial genes that are additionally regulated by long non-coding RNAs (lncRNAs). To date, the expression profile of lncRNA in human term placenta has not been fully established. This study was conducted to characterize the lncRNA expression profile in human term placenta and to verify whether there are differences in the transcriptomic profile between the sex of the fetus and pregnancy multiplicity. RNA-Seq data were used to profile, quantify, and classify lncRNAs in human term placenta. The applied methodology enabled detection of the expression of 4463 isoforms from 2899 annotated lncRNA loci, plus 990 putative lncRNA transcripts from 607 intergenic regions. Those placentally expressed lncRNAs displayed features such as shorter transcript length, longer exon length, fewer exons, and lower expression levels compared to messenger RNAs (mRNAs). Among all placental transcripts, 175,268 were classified as mRNAs and 15,819 as lncRNAs, and 56,727 variants were discovered within unannotated regions. Five differentially expressed lncRNAs (HAND2-AS1, XIST, RP1-97J1.2, AC010084.1, TTTY15) were identified by a sex-bias comparison. Splicing events were detected within 37 genes and 4 lncRNA loci. Functional analysis of cis-related potential targets for lncRNAs identified 2021 enriched genes. It is presumed that the obtained data will expand the current knowledge of lncRNAs in placenta and human non-coding catalogs, making them more contemporary and specific.
Obesity and Aging in the Drosophila Model
Authors: Gáliková Martina , Klepsatel Peter
Published: June 2018

Being overweight increases the risk of many metabolic disorders, but how it affects lifespan is not completely clear. Not all obese people become ill, and the exact mechanism that turns excessive fat storage into a health-threatening state remains unknown. Drosophila melanogaster has served as an excellent model for many diseases, including obesity, diabetes, and hyperglycemia-associated disorders, such as cardiomyopathy or nephropathy. Here, we review the connections between fat storage and aging in different types of fly obesity. Whereas obesity induced by high-fat or high-sugar diet is associated with hyperglycemia, cardiomyopathy, and in some cases, shortening of lifespan, there are also examples in which obesity correlates with longevity. Transgenic lines with downregulations of the insulin/insulin-like growth factor (IIS) and target of rapamycin (TOR) signaling pathways, flies reared under dietary restriction, and even certain longevity selection lines are obese, yet long-lived. The mechanisms that underlie the differential lifespans in distinct types of obesity remain to be elucidated, but fat turnover, inflammatory pathways, and dysregulations of glucose metabolism may play key roles. Altogether, Drosophila is an excellent model to study the physiology of adiposity in both health and disease.
Pheromone-Trap Monitoring System for Pea Leaf Weevil, Sitona lineatus: Effects of Trap Type, Lure Type and Trap Placement within Fields
Authors: Reddy Gadi V. P. , Shrestha Govinda , Miller Debra A. , Oehlschlager A. Cameron
Published: June 2018

The pea leaf weevil, Sitona lineatus, is an important pest of field peas and faba beans worldwide. Present sampling techniques that rely on detection of adult feeding damage are labor intensive, time consuming and require repeated sampling. Semiochemical-based pest monitoring systems could improve pea leaf weevil management. This study, which was conducted in the Golden Triangle region of Montana, tested several factors that potentially might affect capture rates of pheromone-baited traps, including trap and lure type and trap placement. Pheromone-baited pitfall and ramp traps caught significantly more adults than ground or delta traps, in all study areas. Pitfall traps baited with gray rubber septa captured significantly more adults than traps baited with membrane formulations or controls in both pea and lentil fields. In addition, pheromone-baited pitfall traps positioned in the southern part of pea fields captured relatively higher numbers of adults than those placed in northern parts of fields, although this difference was not significant. These findings can be used to improve adult weevil monitoring and should be taken into consideration when developing an integrated pest management program.
Defect Structure and Oxide Ion Conduction of Potassium Ion Substituted CaWO4
Authors: Takai Shigeomi , Shitaune Shinya , Sano Toshifumi , Kawaji Hitoshi , Yabutsuka Takeshi , Esaka Takao , Yao Takeshi
Published: June 2018

We have prepared Ca1−xKxWO4−x/2 solid solutions with the Scheelite-type structure to investigate high-temperature electrochemical properties. Room-temperature X-ray diffraction suggested the solid solution range was x ≤ 0.2, since the second phase presumably of K2WO4 was detected for x = 0.3. For all the substituted samples up to x = 0.4, a large jump in conductivity has been observed around 500 °C. At higher temperatures, oxide ion conduction is found to be predominant even for x = 0.4, exceeding the solution limit estimated from the room-temperature XRD. The conductivity at high temperature is essentially proportional to the amount of substituted potassium ions up to x = 0.4, indicating that oxide ion conduction is associated with the formed oxide ion vacancy. High-temperature X-ray diffraction detected no apparent change in lattice parameters around 500 °C for x = 0.1, and the remaining second phase seems to be incorporated into the Scheelite lattice at high temperatures.
The Microstructure-Mechanical Properties of Hybrid Fibres-Reinforced Self-Compacting Lightweight Concrete with Perlite Aggregate
Authors: Barnat-Hunek Danuta , Góra Jacek , Andrzejuk Wojciech , Łagód Grzegorz
Published: June 2018

The purpose of this paper is to determine the influence of the lightweight porous perlite aggregate and two widely used types of fibres on the physical and mechanical properties, frost durability and microstructure of self-compacting lightweight concrete (SCLC). The experimental investigation consisted of tests carried out on cubes and prismatic samples made of SCLC and fibres-reinforced SCLC with variable content ranging from 0.5 to 1% of basalt fibres (BF) and/or 0.5% of steel fibres (SF). In this study, two variable contents of fine perlite aggregate were used: 5% and 15%. The workability (the slump-flow and t500 values) in fresh state SCLCs have been done. Extensive data on compressive and flexural tensile strength in bending behaviour, frost resistance and the microstructure including interfacial transition zone (ITZ) were recorded and analysed. The hybrid fibres-reinforced SCLC with perlite aggregate showed a more ductile behaviour compared to that of SCLC without fibres. Fibres bridge cracks during flexural tensile strength test. BF successfully protected porous SCLC against frost attack, whereas SF succumbed to damage.
Optimization of the Iron Ore Direct Reduction Process through Multiscale Process Modeling
Authors: Béchara Rami , Hamadeh Hamzeh , Mirgaux Olivier , Patisson Fabrice
Published: June 2018

Iron ore direct reduction is an attractive alternative steelmaking process in the context of greenhouse gas mitigation. To simulate the process and explore possible optimization, we developed a systemic, multiscale process model. The reduction of the iron ore pellets is described using a specific grain model, reflecting the transformations from hematite to iron. The shaft furnace is modeled as a set of interconnected one-dimensional zones into which the principal chemical reactions (3-step reduction, methane reforming, Boudouard and water gas shift) are accounted for with their kinetics. The previous models are finally integrated in a global, plant-scale, model using the Aspen Plus software. The reformer, scrubber, and heat exchanger are included. Results at the shaft furnace scale enlighten the role of the different zones according to the physico-chemical phenomena occurring. At the plant scale, we demonstrate the capabilities of the model to investigate new operating conditions leading to lower CO2 emissions.
Symmetries and Invariants for Non-Hermitian Hamiltonians
Authors: Simón Miguel , Buendía Álvaro , Muga J.
Published: June 2018

We discuss Hamiltonian symmetries and invariants for quantum systems driven by non-Hermitian Hamiltonians. For time-independent Hermitian Hamiltonians, a unitary or antiunitary transformation AHA† that leaves the Hamiltonian H unchanged represents a symmetry of the Hamiltonian, which implies the commutativity [H,A]=0 and, if A is linear and time-independent, a conservation law, namely the invariance of expectation values of A. For non-Hermitian Hamiltonians, H† comes into play as a distinct operator that complements H in generalized unitarity relations. The above description of symmetries has to be extended to include also A-pseudohermiticity relations of the form AH=H†A. A superoperator formulation of Hamiltonian symmetries is provided and exemplified for Hamiltonians of a particle moving in one-dimension considering the set of A operators that form Klein’s 4-group: parity, time-reversal, parity&time-reversal, and unity. The link between symmetry and conservation laws is discussed and shown to be richer and subtler for non-Hermitian than for Hermitian Hamiltonians.
A prospective comparison of double- and single-bundle anterior cruciate ligament reconstructions
Authors: Gudas Rimtautas , Smailys Alfredas , Vostrugina Kristina , Tamoðiûnas Ramûnas , Simonaitis Donatas , Kalesinskas Romas
Published: June 2018

Objective. A nonrandomized clinical study was performed to compare the clinical and radiological outcome between double-bundle and single-bundle anterior cruciate ligament (ACL) reconstructions with semitendinosus tendon in athletes. Material and methods. We examined 70 patients with unilateral anterior cruciate ligament injury. They were followed up for a mean of 24 months. Each group of 35 patients underwent either double- or singlebundle ACL reconstruction. The mean age of the patients was 24.7 years (range, 18–35 years). There were no differences between two groups regarding age at surgery, sex, follow-up period, period before surgery, combined meniscus injuries, athletic activity level, and International Knee Documentation Committee (IKDC) and Tegner scores. All patients followed the same postoperative program. They were evaluated using manual knee laxity tests, knee extension and flexion strength testing. General knee condition was evaluated by the IKDC and Tegner scores. Results. The results were excellent and good in 32 (91.4%) patients after double-bundle ACL reconstruction and in 30 (85.7%) patients after single-bundle ACL reconstruction, evaluating by IKDC system. However, statistical analysis showed no significant difference between the two groups regarding all IKDC-categorized data (P=0.87). The average scores of Tegner activity in double-bundle and single-bundle groups were 8.0 and 8.1, respectively. Conclusions. This trial showed no significant difference between the double and single-bundle ACL repairs.
Venous thromboembolism and prophylaxis in cancer patients
Authors: Paškauskas Saulius , Pundzius Juozas , Barauskas Giedrius
Published: June 2018

Venous thromboembolism is a serious complication in patients with cancer. The seriousness of venous thromboembolism as a complication in cancer patients is becoming recognized as an important medical issue. Venous thromboembolism is a multifactorial disease associated with vascular endothelial damage, stasis of blood flow, and hypercoagulation. Preexisting morbidity, mutations of factor V Leiden or prothrombin 20210A, type of cancer, presence of metastases, use of central venous access, surgery, anesthesia, etc., increase the risk of venous thromboembolism. The patients with malignancies have a 7-fold increase in the risk of venous thromboembolism compared with individuals without cancer. Venous thromboembolism is the second most common cause of mortality in cancer patients. Venous thromboembolism is the most common cause of death at 30 days after surgery in patients undergoing surgery for cancer. Venous thromboembolism caused death in 46.3% of the cases after surgery for cancer. The Geneva prognostic index identified predictive factors for an adverse outcome, and the American College of Chest Physicians (ACCP) has suggested the guidelines for the prevention of venous tromboembolism in cancer patients. Cancer patients should receive appropriate venous thromboembolism prophylaxis. The methods used for venous thromboembolism prophylaxis are mechanical, pharmacological, or a combination of both. Well-timed thromboprophylaxis may protect patients from venous thromboembolism, early lethal outcome and even influence survival.
Nonadherence to medications among pharmacy clients and their attitude toward medications kept a medicine cabinet at home
Authors: Daukšienė Jurgita , Radžiūnas Raimondas
Published: June 2018

Objectives. To assess self-reported adherence among pharmacy clients and to detect if there are any differences in medication use or storage between genders. Material and methods. The data were collected by means of questionnaires. A standard 45-item questionnaire was developed and used to assess adherence and nonadherence to medications. It was distributed to all pharmacy customers who entered pharmacies chosen by us. Results. Of the 162 (45 men and 117 women) pharmacy clients participating in the study, 36.42% were considered nonadherent. No significant difference in adherence rates between genders was observed (P>0.05). Women more often named themselves as being responsible for a medicine cabinet at home (P<0.001) and used more sources of information on medications (P<0.05). Women also more frequently checked the expiry date of medications in a medicine cabinet home than men (P<0.05). Conclusions. The problem of nonadherence to medications exists among pharmacy clients. There is no significant difference in the rate of self-reported adherence between male and female pharmacy clients. Women are the ones named as responsible for a medicine cabinet at home. They also are important health decision makers. Women are more interested in information on medicines and their use; therefore, this demand should be satisfied.
Designed Materials by Additive Manufacturing—Impact of Exposure Strategies and Parameters on Material Characteristics of AlSi10Mg Processed by Laser Beam Melting
Authors: Pfaff Aron , Jäcklein Martin , Hoschke Klaus , Wickert Matthias
Published: June 2018

The Laser Beam Melting (LBM) Additive Manufacturing technology for metal processing is based on the local application of an intense laser beam, causing a characteristic microstructure, which can achieve higher mechanical properties than conventionally manufactured equivalents. The material is created incrementally in sections that are processed with different manufacturing parameters. This paper proposes the creation of Designed Materials by varying the manufacturing parameters and exposure strategy in order to induce a gradient or a local change of properties by designing the microstructure. Such materials could also be created by changing the material topology on a micro-, meso-, or macro-scale, or on multiple scales at once. This enables systematic creation of material types like Functionally Graded Materials (FGMs), Metamaterials, or other Designed Materials, in which characteristics can be varied locally in order to create a customized material. To produce such materials by LBM, it is necessary to gain a detailed understanding about the influence of the manufacturing parameters. Experimental studies have been carried out to investigate the melt pool geometry and microstructure resulting from the exposure parameters. Based on the results, parameter sheets have been derived, which support the process of finding optimized parameter sets for a specific purpose. General methods and their ability to influence the material structure and properties were tested and evaluated. Furthermore, the resulting change of the microstructure was analyzed and a first Graded Material was generated and analyzed to show the potential and possibilities for Designed Materials on multiple scales by Laser Beam Melting.
Analysis of Shape Geometry and Roughness of Ti6Al4V Parts Fabricated by Nanosecond Laser Ablation
Authors: Campanelli Sabina Luisa , Lavecchia Fulvio , Contuzzi Nicola , Percoco Gianluca
Published: June 2018

Laser milling is a micro-machining process that uses a laser beam as a tool to remove material through the layer-by-layer ablation mechanism. Generally in laser ablation, the quality of parts is reduced by melt accretions and thermal damage; therefore, this problem is reduced with shorter pulse duration, although ablation efficiency decreases as well. Thus, laser ablation in the nanosecond range still offers a good compromise between process quality and efficiency. Therefore, laser milling with nanosecond laser ablation requires an accurate study to reduce geometric defects induced by the process. The aim of this paper was to study the shape geometry and roughness of Ti6Al4V parts fabricated by laser milling using a nanosecond Nd:YAG laser source. The impact of the laser processing parameters on machining outcomes was studied in order to determine the optimized processing conditions for reducing geometrical defects and improving surface quality. In particular, the influence of average laser power, frequency, and scanning speed was investigated. The geometry of micro-parts was revealed using a 3D digitizing system, the Optimet Mini Conoscan 4000, which combines a non-contact, single-point measuring sensor based on conoscopic holography technology. The use of this measurement technology yielded complete information of the shape geometry and dimensions of the built parts. In addition, the roughness of manufactured surfaces was assessed to complete the analysis.
Recovery of Residual Silver-Bearing Minerals from Low-Grade Tailings by Froth Flotation: The Case of Zgounder Mine, Morocco
Authors: Drif Boujemaa , Taha Yassine , Hakkou Rachid , Benzaazoua Mostafa
Published: June 2018

The need to explore more complex and low-grade silver ores and to develop novel and cost-effective processes to recover silver from waste is becoming an important challenge. This paper aims to characterize old, low-grade, silver tailings generated by the former Zgounder silver mine, located in Morocco. Understanding the mineralogical composition, particularly the silver deportment, was critical to allow the recovery of silver from these tailings. More than 88 samples of low grade tailings were sampled and characterized using chemical and mineralogical techniques. Froth flotation was used to recover silver bearing minerals using a combination of different collectors (dithiophosphate, dialkyl dithiophosphinates, Aero 7518, Aero 7640, alkyl dithiophosphates and potassium butyl-xanthate). The main goal was to optimize the flotation process at a laboratory scale through the testing of different parameters, such as collectors and frother types and dosage, activators and sulphidizing agents, and pH conditions. The characterization results showed that silver content varied between 30 and 440 ppm with an overall average content of 148 ppm. Silver occurs mainly in the form of native silver as well as in association with sulphides, such as acanthite and pyrite. Minor amounts of sphalerite, chalcopyrite, arsenopyrite, and hematite were identified. The flotation results showed the following optimum conditions: particle size of 63 µm, conditioning pH of 8.5, a combination of butyl-xanthate and dithiophosphate as collectors at a dosage of 80 g/t each, a concentration of 200 g/t of the activating agent (CuSO4), 30 g/t of methyl isobutyl carbonyl (MIBC) frother and a duration time of 8 min with slow kinetics. With these optimal conditions, it was possible to achieve a maximum silver recovery yield of 84% with 1745 ppm Ag grade to be cyanided. Moreover, the environmental behavior of the final clean tailings was demonstrated to be inert using Toxicity characteristic leaching procedure (TCLP) leaching tests.
Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization Mass Spectrometry for Therapeutic Drug Monitoring of Metformin and Rosuvastatin in Human Plasma
Authors: Antonopoulos Nikolaos , Machairas Giorgos , Migias George , Vonaparti Ariadni , Brakoulia Vasiliki , Pistos Constantinos , Gennimata Dimitra , Panderi Irene
Published: June 2018

In this work a hydrophilic interaction liquid chromatography/positive ion electrospray mass spectrometric assay (HILIC/ESI-MS) has been developed and fully validated for the quantitation of metformin and rosuvastatin in human plasma. Sample preparation involved the use of 100 μL of human plasma, following protein precipitation and filtration. Metformin, rosuvastatin and 4-[2-(propylamino) ethyl] indoline 2 one hydrochloride (internal standard) were separated by using an X-Bridge-HILIC BEH analytical column (150.0 × 2.1 mm i.d., particle size 3.5 μm) with isocratic elution. A mobile phase consisting of 12% (v/v) 15 mM ammonium formate water solution in acetonitrile was used for the separation and pumped at a flow rate of 0.25 mL min−1. The linear range of the assay was 100 to 5000 ng mL−1 and 2 to 100 ng mL−1 for metformin and rosuvastatin, respectively. The current HILIC-ESI/MS method allows for the accurate and precise quantitation of metformin and rosuvastatin in human plasma with a simple sample preparation and a short a chromatographic run time (less than 15 min). Plasma samples from eight patients were further analysed proving the capability of the proposed method to support a wide range of clinical studies.
Antifungal and Cytotoxic Activities of Sixty Commercially-Available Essential Oils
Authors: Powers Chelsea N. , Osier Jessica L. , McFeeters Robert L. , Brazell Carolyn Brianne , Olsen Emily L. , Moriarity Debra M. , Satyal Prabodh , Setzer William N.
Published: June 2018

There is an urgent and unmet need for new antifungal therapies. Global fungal infection rates continue to rise and fungal infections pose increasing burdens on global healthcare systems. Exacerbating the situation, the available antifungal therapeutic arsenal is limited and development of new antifungals has been slow. Current antifungals are known for unwanted side effects including nephrotoxicity and hepatotoxicity. Thus, the need for new antifungals and new antifungal targets is urgent and growing. A collection of 60 commercially-available essential oils has been screened for antifungal activity against Aspergillus niger, Candida albicans, and Cryptococcus neoformans, as well as for cytotoxic activity against MCF-7 and MDA-MB-231 human breast tumor cell lines; the chemical compositions of the essential oils have been determined by gas chromatography-mass spectrometry (GC-MS). Ten essential oils showed remarkable antifungal and cytotoxic activities: Indian, Australian, and Hawaiian sandalwoods; melissa; lemongrass; cilantro; cassia; cinnamon; patchouli; and vetiver.
An Improved Scalable Synthesis of α- and β-Amyrin
Authors: Serbian Immo , Csuk René
Published: June 2018

The synthesis of α- and β-amyrin was accomplished starting from easily accessible starting materials, oleanolic, and ursolic acid. The procedures allow the preparation of β-amyrin in an exceptionally short scalable manner via selective iodation and reduction. For α-amyrin, a different synthetic approach had to be chosen providing access to α-amyrin in medium-to-large scale.
Synergistic Effect of Bioactive Anticarcinogens from Soybean on Anti-Proliferative Activity in MDA-MB-231 and MCF-7 Human Breast Cancer Cells In Vitro
Authors: Zhu Yingying , Yao Yang , Shi Zhenxing , Everaert Nadia , Ren Guixing
Published: June 2018

Consumption of soybean products has been implicated in the prevention of breast cancer. This study provides insights into the anti-proliferative activity of 12 anticarcinogens from soybean by single or two-way combination treatment against MCF-7 and MDA-MB-231 human breast cancer cells. Results showed that genistein, daidzein, glycitein, genistin and dainzin showed stronger anti-proliferative activity against MCF-7 cells with EC50 values of 66.98 ± 4.87 μM, 130.14 ± 2.10 μM, 190.67 ± 5.65 μM, 72.82 ± 2.66 μM and 179.21 ± 6.37 μM, respectively. There is a synergistic effect of combination treatment of genistin plus daidzin in MCF-7 cells with combination index at inhibition of 50% (CI50) of 0.89 ± 0.12. Genistein, glycitein, genistin and β-sitosterol were demonstrated to have a stronger anti-proliferative activity against MDA-MB-231 cells with EC50 values of 93.75 ± 5.15 μM, 142.67 ± 5.88 μM, 127.82 ± 4.70 μM and 196.28 ± 4.45 μM. The synergistic effect was observed in the mixture of genistein plus genistin, genistein plus β-sitosterol or β-sitosterol plus genistin with CI50 values of 0.56 ± 0.13, 0.54 ± 0.20 and 0.45 ± 0.12, respectively. These bioactive anticarcinogens were able to inhibit invasion and migration of breast cancer cells and the combination treatments enhanced the inhibitory effect. Regulation of PI3K/Akt/mTORpathway seems to be the main mechanisms involved in the anticancer activity.
High Performance Ultrathin MoO3/Ag Transparent Electrode and Its Application in Semitransparent Organic Solar Cells
Authors: Shi Linlin , Cui Yanxia , Gao Yupeng , Wang Wenyan , Zhang Ye , Zhu Furong , Hao Yuying
Published: June 2018

In this paper, we demonstrate high performance ultrathin silver (Ag) transparent electrodes with a thin MoO3 nucleation layer based on the thermal evaporation method. The MoO3/Ag transparent electrodes fabricated at different deposition rates were compared systematically on aspects of the transmission spectrum, surface resistance, and surface morphology. Our study indicates that with the presence of the MoO3 nucleation layer, an Ag film of only 7 nm thick can achieve percolation and the film is porous instead of forming isolated islands. In addition, the increase of the deposition rate can yield obvious improvement of the surface morphology of the Ag film. Specifically, with the help of a 1 nm thick MoO3 nucleation layer, the Ag film of 9 nm thick realized under the deposition rate of 0.7 nm/s has a surface resistance of about 20 ohm/sq and an average transmittance in the visible light range reaching 74.22%. Such a high performance of transmittance is superior to the reported results in the literature, which inevitably suffer obvious drop in the long wavelength range. Next, we applied the ultrathin MoO3/Ag transparent electrode in organic solar cells. The optimized semitransparent organic solar cell displays a power conversion efficiency of 2.76% and an average transmittance in the visible range of 38% when light is incident from the Ag electrode side.
Association between Dietary Inflammatory Index, C-Reactive Protein and Metabolic Syndrome: A Cross-Sectional Study
Authors: Ren Zhongxia , Zhao Ai , Wang Yan , Meng Liping , Szeto Ignatius Man-Yau , Li Ting , Gong Huiting , Tian Zixing , Zhang Yumei , Wang Peiyu
Published: June 2018

Increased prevalence of metabolic syndrome (MetS) has become a global major public health problem. Chronic low-grade inflammation associated with diet was found to play an import role in the development of MetS, although further studies are needed. The main purpose of this study was to explore the association between the dietary inflammatory index (DII), C-reactive protein (CRP) as a sign of inflammation status, and MetS. A total of 1712 participants from eight cities in China were included. Sociodemographic and health-related information was collected by a self-administrated questionnaire. Anthropometric information and fasting blood samples were collected for identification of MetS. DII scores were computed based on one time 24-h dietary recall. No significant association between MetS and DII was observed except for the blood pressure component of MetS (OR T3 versus T1 = 1.40; 95% CI: 1.03 to 1.89). A significant increased prevalence for MetS was observed for higher CRP (OR = 1.66; 95% CI: 1.26 to 2.18), as well as four out of five of MetS components. In stratified analyses by sex, the associations between DII/CRP and MetS among women, but not men, are comparable to the whole sample. In addition, Both the 2nd and 3rd tertile of the DII had a higher CRP level (β-Coefficients T2 versus T1 = 0.086, 95% CI: 0.004 to 0.167; β-Coefficients T3 versus T1 = 0.145, 95% CI: 0.045 to 0.245) among subjects with MetS. Participants with higher DII scores reported a higher degree of “Shanghuo” (p = 0.007), which is a traditional concept characterized by “redness, swelling, fever and pain” in Chinese Medicine. This study suggested a close association between CRP and MetS, while the association between the DII and MetS was limited. DII was only specifically associated with CRP at a higher level among participants with MetS.
Diet-Quality and Socio-Demographic Factors Associated with Non-Nutritive Sweetener Use in the Australian Population
Authors: Grech Amanda , Kam Chi , Gemming Luke , Rangan Anna
Published: June 2018

Non-nutritive sweeteners (NNS) are used in the food supply to replace sugar and/or to reduce dietary energy intake. The aim of this research was to assess the consumption prevalence and food sources of NNS in the Australian population. Food group and nutrient intakes were assessed to compare diet quality of NNS consumers and non-consumers. Secondary analysis of the Australian National Nutrition and Physical Activity Survey, 2011/12 was conducted (n = 12,435) after identifying all NNS products consumed in the population. The proportion of participants that reported intake of NNS per day was 18.2% for adults (19+ years), and 8.5% for children (2–18 years), with the most common food sources being carbonated soft drinks, tabletop sweeteners, and yoghurt. Characteristics associated with NNS consumption in adults included being female, higher body mass index (BMI), self-reported diabetes status, and being on a weight-loss diet. For adults, NNS consumers had lower free sugar intake but energy intake did not differ from non-consumers. However, for children, no differences in free sugar or energy intake were observed between consumers and non-consumers. While these results support the use of NNS in reducing sugar intake, these data suggest compensatory increases in energy intake may occur.
Contribution of Epstein–Barr Virus Latent Proteins to the Pathogenesis of Classical Hodgkin Lymphoma
Authors: Vrzalikova Katerina , Sunmonu Taofik , Reynolds Gary , Murray Paul
Published: June 2018

Pathogenic viruses have evolved to manipulate the host cell utilising a variety of strategies including expression of viral proteins to hijack or mimic the activity of cellular functions. DNA tumour viruses often establish latent infection in which no new virions are produced, characterized by the expression of a restricted repertoire of so-called latent viral genes. These latent genes serve to remodel cellular functions to ensure survival of the virus within host cells, often for the lifetime of the infected individual. However, under certain circumstances, virus infection may contribute to transformation of the host cell; this event is not a usual outcome of infection. Here, we review how the Epstein–Barr virus (EBV), the prototypic oncogenic human virus, modulates host cell functions, with a focus on the role of the EBV latent genes in classical Hodgkin lymphoma.
Improving the Estimation of Daily Aerosol Optical Depth and Aerosol Radiative Effect Using an Optimized Artificial Neural Network
Authors: Qin Wenmin , Wang Lunche , Lin Aiwen , Zhang Ming , Bilal Muhammad
Published: June 2018

Aerosols can absorb and scatter surface solar radiation (SSR), which is called the aerosol radiative forcing effect (ARF). Great efforts have been made for the estimation of the aerosol optical depth (AOD), SSR and ARF using meteorological measurements and satellite observations. However, the accuracy, and spatial and temporal resolutions of these existing AOD, SSR and ARF models should be improved to meet the application requirements, due to the uncertainties and gaps of input parameters. In this study, an optimized back propagation (BP) artificial neural network (Genetic_BP) was developed for improving the estimation of the AOD values. The retrieved AOD values using the Genetic_BP model and meteorological measurements at China Meteorological Administration (CMA) stations were used to calculate SSR and bottom of the atmosphere (BOA) ARF (ARFB) using Yang’s Hybrid model (YHM). The result show that the Genetic_BP could be used for estimating AOD values with high accuracy (R = 0.866 for CASNET (China Aerosol Remote Sensing Network) stations and R = 0.865 for AERONET (Aerosol Robotic Network) stations). The estimated SSR also showed a good agreement with SSR measurements at 96 CMA radiation stations, with RMSE, MAE, R and R2 of 29.27%, 23.77%, 0.948, and 0.899, respectively. The estimated ARFB values are also highly correlated with the AERONET ARFB ones with RMSE, MAE, R and R2 of −35.47%, −25.33%, 0.843, and 0.711, respectively. Finally, the spatial and temporal variations of AOD, SSR, and ARFB values over Mainland China were investigated. Both AOD and SSR values are generally higher in summer than in other seasons. The ARFB are generally stronger in spring and summer than in other seasons. The ranges for the monthly mean AOD, SSR and ARFB values over Mainland China are 0.183–0.333, 10.218–24.196 MJ m−2day−1 and −2.986 to −1.244 MJ m−2day−1, respectively. The Qinghai-Tibetan Plateau has always been an area with the highest SSR, the lowest AOD and the weakest ARFB. In contrast, the Sichuan Basin has always been an area with low SSR, high AOD, and strong ARFB. The newly proposed AOD model may be of vital importance for improving the accuracy and computational efficiency of AOD, SSR and ARFB estimations for solar energy applications, ecological modeling, and energy policy.
Assessment of Satellite and Radar Quantitative Precipitation Estimates for Real Time Monitoring of Meteorological Extremes Over the Southeast of the Iberian Peninsula
Authors: Cánovas-García Fulgencio , García-Galiano Sandra , Alonso-Sarría Francisco
Published: June 2018

Quantitative Precipitation Estimates (QPEs) obtained from remote sensing or ground-based radars could complement or even be an alternative to rain gauge readings. However, to be used in operational applications, a validation process has to be carried out, usually by comparing their estimates with those of a rain gauges network. In this paper, the accuracy of three QPEs are evaluated for three extreme precipitation events in the last decade in the southeast of the Iberian Peninsula. The first QPE is PERSIANN-CCS (Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks - Cloud Classification System) , a satellite-based QPE. The second and the third are QPEs from a meteorological radar with Doppler capabilities that works in the C band. Pixel-to-point comparisons are made between the values offered by the QPEs and those obtained by two networks of rain gauges. The results obtained indicate that all the QPEs were well below the rain gauge values in extreme rainfall time slots. There seems to be a weak linear association between the value of the discrepancies and the precipitation value of the QPEs. The main conclusion, assuming the information from the rain gauges as ground truth, is that neither PERSIANN-CCS nor radar, without empirical calibration, are acceptable QPEs for the real-time monitoring of meteorological extremes in the southeast of the Iberian Peninsula.
HyTexiLa: High Resolution Visible and Near Infrared Hyperspectral Texture Images
Authors: Khan Haris , Mihoubi Sofiane , Mathon Benjamin , Thomas Jean-Baptiste , Hardeberg Jon
Published: June 2018

We present a dataset of close range hyperspectral images of materials that span the visible and near infrared spectrums: HyTexiLa (Hyperspectral Texture images acquired in Laboratory). The data is intended to provide high spectral and spatial resolution reflectance images of 112 materials to study spatial and spectral textures. In this paper we discuss the calibration of the data and the method for addressing the distortions during image acquisition. We provide a spectral analysis based on non-negative matrix factorization to quantify the spectral complexity of the samples and extend local binary pattern operators to the hyperspectral texture analysis. The results demonstrate that although the spectral complexity of each of the textures is generally low, increasing the number of bands permits better texture classification, with the opponent band local binary pattern feature giving the best performance.
3D Magnetic Field Reconstruction Methodology Based on a Scanning Magnetoresistive Probe
Authors: Richheimer Filipe , Costa Margaret , Leitao Diana C. , Gaspar João , Cardoso Susana , Freitas Paulo P.
Published: June 2018

The present work provides a detailed description on quantitative 3D magnetic field reconstruction using a scanning magnetoresistance microscopy setup incorporating a 19.5 μm × 2.5 μm magnetoresistive sensor. Therefore, making use of a rotation stage, 11 nm thick ferromagnetic CoFe elements with 20 μm × 5 μm planar size were measured along different sensor axes and converted into cartesian coordinate magnetic field components by use of the analytical coordinate transform equations. The reconstruction steps were followed and validated by numerical simulations based on a field averaging model caused by a non-negligible sensor volume. Detailed in-plane magnetic component reconstruction with ability to reconstruct sub-micrometer features is achieved. A discussion on the limiting factors for optimal resolution is presented.
Analysis and Improvement of a Dual-Core Photonic Crystal Fiber Sensor
Authors: Bing Pibin , Huang Shichao , Sui Jialei , Wang Hua , Wang Zhiyong
Published: June 2018

The characteristics of the dual-core photonic crystal fiber (PCF) sensor are studied using the finite element method (FEM), and the structure is improved according to the numerical simulation results. The results show that whether or not the four large air holes far away from the geometry center of the PCF are filled with analyte has no influence on the wavelength sensitivity of the sensor which means those holes can be replaced by small air holes. The wavelength sensitivity can be tuned by adjusting the sizes of the other large air holes which are as for liquid holes. The dynamic detection range of the refractive index (RI) is from 1.33 to 1.51. In particular, high linearity is obtained in the range of 1.44 to 1.51. The sensitivity is as high as 6021 nm/RIU when the liquid holes are the smallest. When liquid holes are tangential with the envelope of first layer air holes, the wavelength sensitivity is 4028 nm/RIU, and the coefficient of determination (R2) is 0.99822 when the RI of the analyte varies from 1.44 to 1.51 which shows that high sensitivity and good linearity are both obtained.
High Dynamic Micro Vibrator with Integrated Optical Displacement Detector for In-Situ Self-Calibration of MEMS Inertial Sensors
Authors: Du Yi-Jia , Yang Ting-Ting , Gong Dong-Dong , Wang Yi-Cheng , Sun Xiang-Yu , Qin Feng , Dai Gang
Published: June 2018

The scale factor drifts and other long-term instability drifts of Micro-Electro-Mechanical System (MEMS) inertial sensors are the main contributors of the position and orientation errors in high dynamic environments. In this paper, a novel high dynamic micro vibrator, which could provide high acceleration and high angular rate rotation with integrated optical displacement detector, is proposed. Commercial MEMS inertial sensors, including 3-axis accelerometer and 6-axis inertial measurement unit which is about 3 mm * 3 mm * 1 mm with 19 mg, could be bonded on the vibration platform of the micro vibrator to perform in-situ during the self-calibration procedure. The high dynamic micro vibrator is fabricated by a fully-integrated MEMS process, including lead zirconate titanate (PZT) film deposition, PZT and electrodes patterning, and structural ion etching. The optical displacement detector, using vertical-cavity surface-emitting laser (VCSEL) and photoelectric diodes (PD), is integrated on the top of the package to measure the 6-DOF vibrating displacement with the detecting resolution of 150 nm in the range of 500 μm. The maximum out-of-plane acceleration of the z-axis vibrating platform loaded with commercial 3-axis accelerometer (H3LIS331DL) achieves above 16 g and the maximum angular velocity achieves above 720°/s when the driving voltage is ±6 V.
Antidepressant-Like Effect of Lippia sidoides CHAM (Verbenaceae) Essential Oil and Its Major Compound Thymol in Mice
Authors: Parente Michele Sanford Rangel , Custódio Feliphy Rodrigues , Cardoso Nayara Araújo , Lima Márcia Jordana Araújo , Melo Tiago Sousa de , Linhares Maria Isabel , Siqueira Rafaelly Maria Pinheiro , Nascimento Aristides Ávilo do , Catunda Júnior Francisco Eduardo Aragão , Melo Carla Thiciane Vasconcelos de
Published: June 2018

Depression is a common disease affecting more than 300 million people worldwide. Since Lippia sidoides has shown central nervous system effects in previous works, we aimed to investigate the effect of L. sidoides essential oil and its major compound, thymol on a corticosterone-induced depression model in mice. Male mice (20–25 g) received corticosterone (20 mg/kg, subcutaneously), once a day for 22 days. From the 16th day on, mice were grouped to receive either corticosterone or L. sidoides essential oil (100 and 200 mg/kg), or thymol (25 and 50 mg/kg) or fluoxetine (35 mg/kg) by gavage. The forced swimming test, tail suspension, open field, elevated plus maze and sucrose preference tests were performed from the 19th to 22nd day. Data were analyzed by ANOVA followed by the Student-Newman-Keuls as a post hoc test and the results were considered significant when p < 0.05. It was shown that L. sidoides essential oil, thymol and fluoxetine decreased the immobility time in the tail suspension and forced swimming tests and none of these altered locomotor activity in the open field test. However, the drugs increased the amount of grooming. In the elevated plus maze, all drugs increased the number of entries and the time of permanence in the open arms. In the sucrose preference test, the L. sidoides essential oil, thymol and fluoxetine reversed anhedonia. These results suggest that the thymol and L. sidoides essential oil have an antidepressant-like effect, similar to fluoxetine. However, future studies should be encouraged to enhance understanding of the effects of essential oil and thymol for the treatment of depression.
Synergy Degree Evaluation Based on Synergetics for Sustainable Logistics Enterprises
Authors: Huang Juan , Shuai Yuhong , Liu Qi , Zhou Hang , He Zhenggang
Published: June 2018

Today, logistics activities have become a major source of pollution that affects the environment and green logistics is becoming a hot topic. A logistics company’s operating strategy determines the direction of logistics activities and impact degree of logistics activities on the environment. And in logistics enterprises, there is a direct relationship between efficiency and collaboration as collaboration can reduce logistics costs and the negative impact of the bullwhip effect and increase the service level. Synergy degree evaluation, therefore, is crucial to analyze collaboration, identify vulnerabilities, promote development and is also a key step in building a green logistics system. This paper employs Synergetics to comprehensively evaluate the synergy degree in sustainable logistics enterprises. First, Synergetics is introduced to evaluate the synergy degree of sustainable logistics enterprises and the basic Synergetics principles are presented. Second, based on the Law of the Factors of Production, the synergy elements and logistics enterprise content is divided into three main factors: subject elements, object elements and facility and equipment elements. Then, a measurement model and framework for the synergy degree of logistics enterprises is built. Finally, a case study is given to verify the effectiveness of the proposed model and framework. It was found that the higher the logistics enterprise synergy degree, the higher the efficiency.