bluuebunny/crossref_metadata_2025 huggingface dataset:
Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation
Authors: Okada Takuya , Keino-Masu Kazuko , Nagamine Satoshi , Kametani Fuyuki , Ohto Tatsuyuki , Hasegawa Masato , van Kuppevelt Toin H. , Kunita Satoshi , Takahashi Satoru , Masu Masayuki
Published: October 2017

Heparan sulfate (HS) has been implicated in a wide range of cell signaling. Here we report a novel mechanism in which extracellular removal of 6-O-sulfate groups from HS by the endosulfatases, Sulf1 and Sulf2, is essential for axon guidance during development. In Sulf1/2 double knockout (DKO) mice, the corticospinal tract (CST) was dorsally displaced on the midbrain surface. In utero electroporation of Sulf1/2 into radial glial cells along the third ventricle, where Sulf1/2 mRNAs are normally expressed, rescued the CST defects in the DKO mice. Proteomic analysis and functional testing identified Slit2 as the key molecule associated with the DKO phenotype. In the DKO brain, 6-O-sulfated HS was increased, leading to abnormal accumulation of Slit2 protein on the pial surface of the cerebral peduncle and hypothalamus, which caused dorsal repulsion of CST axons. Our findings indicate that postbiosynthetic desulfation of HS by Sulfs controls CST axon guidance through fine-tuning of Slit2 presentation.
Nacre-mimic Reinforced Ag@reduced Graphene Oxide-Sodium Alginate Composite Film for Wound Healing
Authors: Yan Xu , Li Fei , Hu Kang-Di , Xue Jingzhe , Pan Xiao-Feng , He Tao , Dong Liang , Wang Xiang-Ying , Wu Ya-Dong , Song Yong-Hong , Xu Wei-Ping , Lu Yang
Published: October 2017

With the emerging of drug-resistant bacterial and fungal pathogens, there raise the interest of utilizing versatile antimicrobial biomaterials to treat the acute wound. Herein, we report the spraying mediated assembly of a bio-inspired Ag@reduced graphene-sodium alginate (AGSA) composite film for effective wound healing. The obtained film displayed lamellar microstructures similar to the typical “brick-and-mortar” structure in nacre. In this nacre-mimic structure, there are abundant interfacial interactions between nanosheets and polymeric matrix, leading to remarkable reinforcement. As a result, the tensile strength, toughness and Young’s modulus have been improved 2.8, 2.3 and 2.7 times compared with pure sodium alginate film, respectively. In the wound healing study, the AGSA film showed effective antimicrobial activities towards Pseudomonas aeruginosa, Escherichia coli and Candida albicans, demonstrating the ability of protecting wound from pathogenic microbial infections. Furthermore, in vivo experiments on rats suggested the effect of AGSA film in promoting the recovery of wound sites. According to MTT assays, heamolysis evaluation and in vivo toxicity assessment, the composite film could be applied as a bio-compatible material in vitro and in vivo. Results from this work indicated such AGSA film has promising performance for wound healing and suggested great potential for nacre-mimic biomaterials in tissue engineering applications.
Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes
Authors: Kim Han Sol , Yoon Jung Won , Li Hongliang , Jeong Geun Ok , Park Jin Ju , Shin Sung Eun , Jang Il Ho , Kim Jae Ho , Park Won Sun
Published: October 2017

Cardiomyocytes differentiated from human pluripotent stem cells provide promising tools for screening of cardiotoxic drugs. For evaluation of human pluripotent stem cell-derived cardiomyocytes for cardiotoxicity test, in the present study, human embryonic stem cells (hESCs) were differentiated to cardiomyocytes, followed by metabolic selection to enrich the differentiated cardiomyocytes. The highly purified hESC-derived cardiomyocytes (hESC-CMs) expressed several cardiomyocyte-specific markers including cTnT, MLC2a, and α-SA, but not pluripotency markers, such as OCT4 and NANOG. Patch clamp technique and RT-PCR revealed the expression of cardiomyocyte-specific Na+, Ca2+, and K+ channels and cardiac action potential in hESC-CMs. To explore the potential use of hESC-CMs as functional cardiomyocytes for drug discovery and cardiotoxicity screening, we examined the effects of bisindolylmaleimide (BIM) (I), which inhibits native cardiac Ca2+ channels, on the Ca2+ channel activity of hESC-CMs. We observed a similar response for the BIM (I)-induced modulation of Ca2+ channels between hESC-CMs and native cardiomyocytes through L-type Ca2+ channel current. These results suggest that hESC-CMs can be useful for evaluation of pharmaceutical efficacy and safety of novel drug candidate in cardiac research.
No evidence of a cleaning mutualism between burying beetles and their phoretic mites
Authors: Duarte Ana , Cotter Sheena C. , De Gasperin Ornela , Houslay Thomas M. , Boncoraglio Giuseppe , Welch Martin , Kilner Rebecca M.
Published: October 2017

Burying beetles (Nicrophorus vespilloides) breed on small vertebrate carcasses, which they shave and smear with antimicrobial exudates. Producing antimicrobials imposes a fitness cost on burying beetles, which rises with the potency of the antimicrobial defence. Burying beetles also carry phoretic mites (Poecilochirus carabi complex), which breed alongside them on the carcass. Here we test the novel hypothesis that P. carabi mites assist burying beetles in clearing the carcass of bacteria as a side-effect of grazing on the carrion. We manipulated the bacterial environment on carcasses and measured the effect on the beetle in the presence and absence of mites. With next-generation sequencing, we investigated how mites influence the bacterial communities on the carcass. We show that mites: 1) cause beetles to reduce the antibacterial activity of their exudates but 2) there are no consistent fitness benefits of breeding alongside mites. We also find that mites increase bacterial diversity and richness on the carcass, but do not reduce bacterial abundance. The current evidence does not support a cleaning mutualism between burying beetles and P. carabi mites, but more work is needed to understand the functional significance and fitness consequences for the beetle of mite-associated changes to the bacterial community on the carcass.
Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity
Authors: Orlando Marta , Ravasenga Tiziana , Petrini Enrica Maria , Falqui Andrea , Marotta Roberto , Barberis Andrea
Published: October 2017

Both excitatory and inhibitory synaptic contacts display activity dependent dynamic changes in their efficacy that are globally termed synaptic plasticity. Although the molecular mechanisms underlying glutamatergic synaptic plasticity have been extensively investigated and described, those responsible for inhibitory synaptic plasticity are only beginning to be unveiled. In this framework, the ultrastructural changes of the inhibitory synapses during plasticity have been poorly investigated. Here we combined confocal fluorescence microscopy (CFM) with high resolution scanning electron microscopy (HRSEM) to characterize the fine structural rearrangements of post-synaptic GABAA Receptors (GABAARs) at the nanometric scale during the induction of inhibitory long-term potentiation (iLTP). Additional electron tomography (ET) experiments on immunolabelled hippocampal neurons allowed the visualization of synaptic contacts and confirmed the reorganization of post-synaptic GABAAR clusters in response to chemical iLTP inducing protocol. Altogether, these approaches revealed that, following the induction of inhibitory synaptic potentiation, GABAAR clusters increase in size and number at the post-synaptic membrane with no other major structural changes of the pre- and post-synaptic elements.
Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
Authors: Lu Jianfei , Xu Dan , Jiang Yousheng , Kong Shanyun , Shen Zhaoyuan , Xia Siyao , Lu Liqun
Published: October 2017

MicroRNAs (miRNAs) are small, non-coding single stranded RNAs that play crucial roles in numerous biological processes. Vertebrate herpesviruses encode multiple viral miRNAs that modulate host and viral genes. However, the roles of viral miRNAs in lower vertebrates have not been fully determined. Here, we used high-throughput sequencing to analyse the miRNA and mRNA expression profiles of Carassius auratus gibelio in response to infection by cyprinid herpesvirus 2 (CyHV-2). RNA sequencing obtained 26,664 assembled transcripts, including 2,912 differentially expressed genes. Based on small RNA sequencing and secondary structure predictions, we identified 17 CyHV-2 encoded miRNAs, among which 14 were validated by stem-loop quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and eight were validated by northern blotting. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of miRNAs-mRNA pairs revealed diverse affected immune signalling pathways, including the RIG-I-like receptor and JAK-STAT pathways. Finally, we presented four genes involved in RIG-I-like pathways, including host gene IRF3, RBMX, PIN1, viral gene ORF4, which are negatively regulated by CyHV-2 encoded miRNA miR-C4. The present study is the first to provide a comprehensive overview of viral miRNA-mRNA co-regulation, which might have a key role in controlling post-transcriptomic regulation during CyHV-2 infection.
Comparison of four automated microbiology systems with 16S rRNA gene sequencing for identification of Chryseobacterium and Elizabethkingia species
Authors: Lin Jiun-Nong , Lai Chung-Hsu , Yang Chih-Hui , Huang Yi-Han , Lin Hsiu-Fang , Lin Hsi-Hsun
Published: October 2017

Chryseobacterium and Elizabethkingia species have recently emerged as causative agents in life-threatening infections in humans. We aimed to evaluate the rates at which four common microbial identification systems identify Chryseobacterium and Elizabethkingia species in clinical microbiology laboratories. Based on the results of 16S rRNA gene sequencing, a total of 114 consecutive bacteremic isolates, including 36 (31.6%) C. indologenes, 35 (30.7%) E. anophelis, 22 (19.3%) C. gleum, 13 (11.4%) E. meningoseptica, and other species, were included in this study. The overall concordance between each method and 16S rRNA gene sequencing when identifying Chryseobacterium and Elizabethkingia species was 42.1% for API/ID32, 41.2% for Phoenix 100 ID/AST, 43.9% for VITEK 2, and 42.1% for VITEK MS. Among the 22 C. gleum isolates, only one (4.8%) was correctly identified using VITEK 2 and Phoenix 100 ID/AST, and none were accurately recognized using API/ID32 or VITEK MS. Except for two isolates that were not identified using API/ID32, all E. anophelis isolates were misidentified by all four identification systems as E. meningoseptica. Our results show that these approaches have low accuracy when identifying Chryseobacterium and Elizabethkingia species. Hence, we recommend amending the discrimination rate of and adding non-claimed pathogens to databases of microbial identification systems.
Improved electrical and thermo-mechanical properties of a MWCNT/In–Sn–Bi composite solder reflowing on a flexible PET substrate
Authors: Kim Sang Hoon , Park Min-soo , Choi Joon-Phil , Aranas Clodualdo
Published: October 2017

Multi-walled carbon nanotube (MWCNT)/indium–tin–bismuth (In–Sn–Bi) composite nanostructures in which In–Sn–Bi nanoparticles have been penetrated by the MWCNT arrays were synthesized using a chemical reduction method. The incorporation of 0.6 wt% MWCNTs with high electrical conductivity into the In-based solder resulted in low minimum electrical resistivity (19.9 ± 1.0 µΩ·cm). Despite being reflowed at the relatively low temperature of 110 °C, the composite solder nanostructures were able to form mechanically stable solder bumps on a flexible polyethylene terephthalate (PET) substrate due to the MWCNT arrays with a high thermal conductivity of 3000 W/(m·K) and In–Sn–Bi nanoparticles with a low melting temperature of 98.2 °C. Notably, the composite solder bumps exhibited high flexibility (17.7% resistance increase over 1000 cycles of operation in a bending test) and strong adhesion strength (0.9 N average shear strength in a scratch test) on the plastic substrate because of the presence of mechanically flexible and strong MWCNTs dispersed within the solder matrix materials. These overall properties are due to the improved diffusivity of the composite solder nanostructures by the cover of the In–Sn–Bi nanoparticles along the MWCNT arrays and the network structure formation of the composite solder bumps.
Ethanol Induces Enhanced Vascularization Bioactivity of Endothelial Cell-Derived Extracellular Vesicles via Regulation of MicroRNAs and Long Non-Coding RNAs
Authors: Lamichhane Tek N. , Leung Christopher A. , Douti Lampouguin Yenkoidiok , Jay Steven M.
Published: October 2017

Extracellular vesicles (EVs), such as exosomes, have been identified as regulators of vascular remodeling and have promise as therapeutics for vascularization applications. Towards development of EVs as therapeutics, it has been demonstrated that physiological stimuli of angiogenic phenotypes in EV-producing cells can enhance the potency of EVs for vascularization. The goal of this study was to assess whether ethanol, which induces angiogenic phenotypes in endothelial cells, could be employed to enhance endothelial-derived EV vascularization bioactivity. The results indicate that ethanol conditioning of endothelial cells increases the ability of endothelial EVs to induce a pro-vascularization response. This response is due in part to increased CD34 expression in recipient endothelial cells that may result from downregulation of microRNA-106b in EVs isolated from ethanol-conditioned producer endothelial cells. Further, ethanol-induced upregulation of long non-coding RNAs (lncRNAs) HOTAIR and MALAT1 in endothelial EVs was observed to play a significant role in mediating pro-angiogenic effects of these vesicles. Overall, these studies validate ethanol conditioning as a method to enhance the bioactivity of endothelial EVs via regulation of EV-associated microRNAs (miRNAs) and, especially, lncRNAs. Further, the results suggest that alcohol consumption may activate endothelial EVs towards a pro-vascularization phenotype, which could have implications for alcohol-induced tumor angiogenesis.
A microdialysis-based analytical system for dynamic monitoring of arsenic transformation under microbial activity
Authors: Hsu Keng-Chang , Hsieh Jing-Ru , Chen Ya-Ching , Hsu Pi-Fu , Hung Chih-Chang , Huang Yeou-Lih
Published: October 2017

In this study, a microdialysis (MD) technique was combined with high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for continuous monitoring of the dynamic variations of arsenic species in a microbe-inoculated culture broth.
Selective cytotoxicity and luminescence imaging of cancer cells with a dipicolinato-based EuIIIcomplex
Authors: Monteiro J. H. S. K. , Machado D. , de Hollanda L. M. , Lancellotti M. , Sigoli F. A. , de Bettencourt-Dias A.
Published: October 2017

Four new lanthanide complexes with the ligand dipicNH22−(dipic = dipicolinato) show selective cancer cell toxicity and are used for cell luminescence imaging.
Synthesis, structure and optoelectronic properties of rigid 3D tetraindeno-fused spirofluorene with thioxanthene or dioxothioxanthene substitutes
Authors: Guan Jing , Zhang Fangyuan , Zhang Yijiao , Liu Zhigang , Wu Yanli , Xia Debin
Published: October 2017

This work reports the synthesis and characterization of two blue emitters. Their 3D rigid structures are the appealing feature.
A novel bacteriochlorin–styrylnaphthalimide conjugate for simultaneous photodynamic therapy and fluorescence imaging
Authors: Panchenko Pavel A. , Grin Mikhail A. , Fedorova Olga A. , Zakharko Marina A. , Pritmov Dmitriy A. , Mironov Andrey F. , Arkhipova Antonina N. , Fedorov Yuri V. , Jonusauskas Gediminas , Yakubovskaya Raisa I. , Morozova Natalia B. , Ignatova Anastasia A. , Feofanov Alexey V.
Published: October 2017

The Photosensitizing and fluorescence imaging ability of a bacteriochlorin–naphthalimide conjugate is studied.
Impact of intracellular metallothionein on metal biouptake and partitioning dynamics at bacterial interfaces
Authors: Présent Romain M. , Rotureau Elise , Billard Patrick , Pagnout Christophe , Sohm Bénédicte , Flayac Justine , Gley Renaud , Pinheiro José P. , Duval Jérôme F. L.
Published: October 2017

This study reports the quantitative evaluation of the metal biopartitioning dynamics following biouptake at bacterial interfaces with explicit account of the effects stemming from intracellular metal binding by metallothionein proteins.
Modeling of the oxygen reduction reaction for dense LSM thin films
Authors: Yang Tao , Liu Jian , Yu Yang , Lee Yueh-Lin , Finklea Harry , Liu Xingbo , Abernathy Harry W. , Hackett Gregory A.
Published: October 2017

In the present study, the oxygen reduction reaction mechanism is investigated using numerical methods on a dense thin (La1−xSrx)yMnO3±δ film deposited on a YSZ substrate.
The electron density of delocalized bonds (EDDB) applied for quantifying aromaticity
Authors: Szczepanik Dariusz W. , Andrzejak Marcin , Dominikowska Justyna , Pawełek Barbara , Krygowski Tadeusz M. , Szatylowicz Halina , Solà Miquel
Published: October 2017

In this study the recently developed electron density of delocalized bonds (EDDB) is used to define a new measure of aromaticity in molecular rings.
In situ XAS study of CoBi modified hematite photoanodes
Authors: Xi Lifei , Schwanke Christoph , Zhou Dong , Drevon Dorian , van de Krol Roel , Lange Kathrin M.
Published: October 2017

The first report combining the studies of the PEC performance of a CoBi/hematite photoanode and in situ XAS at the Co K-edge.
Synthesis, structural characterization and scalable preparation of new amino-zinc borates
Authors: Imer M. R. , González M. , Veiga N. , Kremer C. , Suescun L. , Arizaga L.
Published: October 2017

Zinc borates are important materials. We report the preparation of three novel ones: [Zn(NH3)3B4O5(OH)4]·H2O (ZB1), Zn3(H2B3O7)2·2NH3·4H2O (ZB2), and [Zn(NH3)4][B4O5(OH)4]·4H2O (ZB3).
Rhenium tricarbonyl complexes of AIE active tetraarylethylene ligands: tuning luminescence properties and HSA-specific binding
Authors: Gabr Moustafa T. , Pigge F. Christopher
Published: October 2017

A luminescent tetraarylethylene Re(i) bioprobe exhibits enhanced emission upon site II-specific binding to human serum albumin.
Facile synthesis of ECNU-20 (IWR) hollow sphere zeolite composed of aggregated nanosheets
Authors: Zhang Lin , Chen Yunlei , Jiang Jin-Gang , Xu Le , Guo Wenping , Xu Hao , Wen Xiao-Dong , Wu Peng
Published: October 2017

A germanosilicate ECNU-20 (IWR) zeolite, with a nanosheets aggregated hollow structure, was synthesized using a commercially available amine as structure-directing agent.
Facile synthesis of hollow dendritic Ag/Pt alloy nanoparticles for enhanced methanol oxidation efficiency
Authors: Sui Ning , Wang Ke , Shan Xinyao , Bai Qiang , Wang Lina , Xiao Hailian , Liu Manhong , Colvin Vicki L. , Yu William W.
Published: October 2017

Hollow dendritic Ag/Pt alloy nanoparticles were synthesized by a double template method.
Continuous assembly of a polymer on a metal–organic framework (CAP on MOF): a 30 nm thick polymeric gas separation membrane
Authors: Xie Ke , Fu Qiang , Xu Chenglong , Lu Hiep , Zhao Qinghu , Curtain Roger , Gu Dunyin , Webley Paul A. , Qiao Greg G.
Published: October 2017

A 30 nm thick polymeric membrane on a metal–organic framework substrate was fabricated via a bottom-up approach, exhibiting excellent CO2/N2 separation performance.
Enrichment of magnetic particles using temperature and magnetic field gradients induced by benchtop fabricated micro-electromagnets
Authors: Hosseini A. , Philpott D. N. , Soleymani L.
Published: October 2017

Cooperation of thermal and magnetic forces used to drive and capture magnetic particles inside microsystems.
Applications and perspectives of nanomaterials in novel vaccine development
Authors: Shen Yingbin , Hao Tianyao , Ou Shiyi , Hu Churan , Chen Long
Published: October 2017

Vaccines show great potential for both prophylactic and therapeutic use in infections, cancer, and other diseases.
Synergistic antifungal effect of cyclized chalcone derivatives and fluconazole against Candida albicans
Authors: Ahmad Aijaz , Wani Mohmmad Younus , Patel Mrudula , Sobral Abilio J. F. N. , Duse Adriano G. , Aqlan Faisal Mohammed , Al-Bogami Abdullah Saad
Published: October 2017

Cyclized bis-chalcone derivatives show synergistic antifungal interactions with fluconazole by ergosterol biosynthesis inhibition evidenced by down regulation of ERG11 gene expression.
Synthesis and antifungal activity of novel oxazolidin-2-one-linked 1,2,3-triazole derivatives
Authors: Ramírez-Villalva Alejandra , González-Calderón Davir , Rojas-García Roxana I. , González-Romero Carlos , Tamaríz-Mascarúa Joaquín , Morales-Rodríguez Macario , Zavala-Segovia Nieves , Fuentes-Benítes Aydeé
Published: October 2017

Novel oxazolidin-2-one-linked 1,2,3-triazole derivatives (4a–k) were synthesized by straightforward and versatile azide–enolate (3 + 2) cycloaddition.
Facile conversion of coal tar to orange fluorescent carbon quantum dots and their composite encapsulated by liposomes for bioimaging
Authors: Geng Bijiang , Yang Dewen , Zheng Fengfeng , Zhang Chen , Zhan Jing , Li Zhen , Pan Dengyu , Wang Liang
Published: October 2017

Our work has provided a way for the conversion of low-value coal tar into high-value fluorescent carbon materials.
Strain engineering, efficient excitonic photoluminescence, and exciton funnelling in unmodified MoS2 nanosheets
Authors: Mangu Vijay Saradhi , Zamiri Marziyeh , Brueck S. R. J. , Cavallo Francesca
Published: October 2017

The electronic band structure of unmodified multilayer MoS2 is manipulated via dry release in place of nanosheets on textured substrates.
An active structure preservation method for developing functional graphitic carbon dots as an effective antibacterial agent and a sensitive pH and Al(iii) nanosensor
Authors: Hou Peng , Yang Tong , Liu Hui , Li Yuan Fang , Huang Cheng Zhi
Published: October 2017

A facile active structure preservation (ASP) method was developed to prepare self-functional graphitic carbon dots that integrate the features of antibiosis, pH-sensitive emission and detection of Al(iii).
Silk fibroin-assisted exfoliation and functionalization of transition metal dichalcogenide nanosheets for antibacterial wound dressings
Authors: Huang Xiao-Wei , Wei Jing-Jing , Liu Tong , Zhang Xue-Liang , Bai Shu-Meng , Yang Huang-Hao
Published: October 2017

A facile and environmentally friendly method was developed for the efficient exfoliation of TMDs in an aqueous medium by using silk fibroin.
N- to C-sulfonyl photoisomerisation of dihydropyridinones: a synthetic and mechanistic study
Authors: Yeh Pei-Pei , Taylor James E. , Stark Daniel G. , Daniels David S. B. , Fallan Charlene , Walton John C. , Smith Andrew D.
Published: October 2017

The scope and limitations of a photoinitiated N- to C-sulfonyl migration process within a range of dihydropyridinones is assessed.
Novel α-amino acid-derived phase-transfer catalyst application to a highly enantio- and diastereoselective nitro-Mannich reaction
Authors: Liu Yuxin , Wei Zhonglin , Liu Yu , Cao Jungang , Liang Dapeng , Lin Yingjie , Duan Haifeng
Published: October 2017

Very broad substrate generality was observed, and the products were achieved in high enantio-/diastereoselectivities (90–>99.9% ee, 9 : 1 to 11 : 1 dr).
Temperature-responsive hydrogels via the electrostatic interaction of amphiphilic diblock copolymers with pendant-ion groups
Authors: Lee Hye Yun , Park Seung Hun , Kim Jae Ho , Kim Moon Suk
Published: October 2017

In this work, we prepared amphiphilic polyester diblock copolymers with pendant-ion groups and examined the formation of temperature-responsive hydrogels via the electrostatic interaction of cationic or anionic pendant groups.
Single Ni atom incorporated with pyridinic nitrogen graphene as an efficient catalyst for CO oxidation: first-principles investigation
Authors: Wang Mingguang , Wang Zhu
Published: October 2017

We have investigated the potential catalytic activity of a single Ni atom incorporated with pyridinic nitrogen graphene (Ni-3N-G) in CO oxidation with first-principles calculations.
Thermodynamics, functional and structural characterization of inosine–uridine nucleoside hydrolase from Leishmania braziliensis
Authors: Dalberto Pedro Ferrari , Martinelli Leonardo Kras Borges , Bachega Jose Fernando Ruggiero , Timmers Luis Fernando Saraiva Macedo , Pinto Antonio Frederico Michel , Dadda Adilio da Silva , Petersen Guilherme Oliveira , Subtil Fernanda Teixeira , Galina Luiza , Villela Anne Drumond , Pissinate Kenia , Machado Pablo , Bizarro Cristiano Valim , de Souza Osmar Norberto , de Carvalho Filho Edgar Marcelino , Basso Luiz Augusto , Santos Diogenes Santiago
Published: October 2017

Inosine–uridine nucleoside hydrolase fromLeishmania braziliensisis a nonspecific enzyme that contains a disulfide bond not needed for tetramer stabilization.
Facile preparation of high-performance Fe-doped Ce–Mn/TiO2 catalysts for the low-temperature selective catalytic reduction of NOx with NH3
Authors: Xu Quan , Su Rigu , Cao Li , Li Yeqing , Yang Chuanyao , Luo Yan , Street Jason , Jiao Pengcheng , Cai Lulu
Published: October 2017

A Ce–Mn–Fe/TiO2 catalyst has been successfully prepared using a single impregnation method with excellent low-temperature NH3-SCR activity.
3D-printed air-blast microfluidic nozzles for preparing calcium alginate microparticles
Authors: Ye Biao , Xu Hong , Bao Binbin , Xuan Jin , Zhang Li
Published: October 2017

Uniform calcium alginate microparticles were prepared through 3D-printed coaxial air-blast microfluidic nozzles.
Li4Ti5O12 nanosquares@ultrathin carbon nanofilms on a large scale with enhanced properties in lithium-ion batteries
Authors: Xiong Yonglian , Chen Yuwei , Yan Jun , Hou Quanhui , Liu Wei
Published: October 2017

Ultra-thin C-LTO nanosquares with high crystallinity were synthesized on a large scale and their electrochemical performance was measured in 18 650 batteries.
Corrosion properties of steel in 1-butyl-3-methylimidazolium hydrogen sulfate ionic liquid systems for desulfurization application
Authors: Zeng Qian , Zhang Jinwei , Cheng Hongye , Chen Lifang , Qi Zhiwen
Published: October 2017

The corrosivity of [BMIM]HSO4-based systems for desulfurization applications were investigated by weight loss and surface analyses.
Fabrication of electroactive poly(vinylidene fluoride) through non-isothermal crystallization and supercritical CO2 processing
Authors: Lee Ji Eun , Guo Yanting , Lee Richard Eungkee , Leung Siu Ning
Published: October 2017

A new strategy using non-isothermal crystallization and supercritical CO2 processing is found to promote the electroactive phase content in PVDF.
Comparative study on sulfamethoxazole degradation by Fenton and Fe(ii)-activated persulfate process
Authors: Wang Shizong , Wang Jianlong
Published: October 2017

Sulfamethoxazole can be effectively degraded by Fenton and Fe(ii)-activated persulfate. The concentration of oxidant has important effect on the degradation of sulfamethoxazole.
Construction of stable core–shell imprinted Ag-(poly-o-phenylenediamine)/CoFe2O4 photocatalyst endowed with the specific recognition capability for selective photodegradation of ciprofloxacin
Authors: Lu Ziyang , Yu Zehui , Dong Jinbo , Song Minshan , Liu Yang , Liu Xinlin , Fan Di , Ma Zhongfei , Yan Yongsheng , Huo Pengwei
Published: October 2017

The stable core–shell imprinted Ag-POPD/CoFe2O4 photocatalyst not only possessed high photocatalytic activity, but also exhibited the superior specific recognition capability for selective photodegradation of CIP.
Correction: Qualitative and quantitative analysis of fatty acid profiles of Chinese pecans (Carya cathayensis) during storage using an electronic nose combined with chemometric methods
Authors: Jiang Shui , Wang Jun , Sun Yubing
Published: October 2017

Correction for ‘Qualitative and quantitative analysis of fatty acid profiles of Chinese pecans (Carya cathayensis) during storage using an electronic nose combined with chemometric methods’ by Shui Jiang et al., RSC Adv., 2017, 7, 46461–46471.
3D chemical imaging of the brain using quantitative IR spectro-microscopy
Authors: Ogunleke Abiodun , Recur Benoit , Balacey Hugo , Chen Hsiang-Hsin , Delugin Maylis , Hwu Yeukuang , Javerzat Sophie , Petibois Cyril
Published: October 2017

Here, we introduce a unique high-throughput infrared (IR) microscopy method that combines automated image correction and subsequent spectral data analysis for 3D-IR image reconstruction.
A micropatterned elastomeric surface with enhanced frictional properties under wet conditions and its application
Authors: Ko H. , Seong M. , Jeong H. E.
Published: October 2017

A new type of micropatterned surface with arch-shaped microstructures with superior wet frictional properties is proposed with a demonstration of its practical application to automated substrate transportation systems.
An inverted supramolecular amphiphile and its step-wise self-assembly into vesicular networks
Authors: Samanta Krishnananda , Zellermann Elio , Zähres Manfred , Mayer Christian , Schmuck Carsten
Published: October 2017

An inverted supramolecular amphiphile self-assembles into 3D networks in a time-dependent process.
Tuning the aggregation of graphene oxide dispersions to synthesize elastic, low density graphene aerogels
Authors: Hu Kaiwen , Szkopek Thomas , Cerruti Marta
Published: October 2017

Controlled aggregation of graphene oxide leads to the formation of ultralight elastic graphene aerogel.
One-step synthesis of V2O5/Ni3S2 nanoflakes for high electrochemical performance
Authors: Wang Xiuhua , Shi Bo , Wang Xiuqin , Gao Jie , Zhang Chen , Yang Zhenzhen , Xie Huifang
Published: October 2017

The diffusion-controlled storage process is predominant in the total capacitance, attributed to the p–n V2O5/Ni3S2 heterojunctions.
Mesoporous 45S5 bioactive glass: synthesis, in vitro dissolution and biomineralization behavior
Authors: Kumar Anil , Murugavel Sevi , Aditya Anusha , Boccaccini Aldo R.
Published: October 2017

The development of a new generation of biomaterials includes a sol–gel process to obtain glass foams, which is a well established method for CaO–SiO2–P2O5 compositions, but is not yet recognized for Bioglass® containing sodium oxide.
Advanced capability of radially aligned fibrous scaffolds coated with polydopamine for guiding directional migration of human mesenchymal stem cells
Authors: Shin Young Min , Shin Hyeok Jun , Yang Dae-Hyeok , Koh Young-Joo , Shin Heungsoo , Chun Heung Jae
Published: October 2017

Radially aligned fibrous scaffold guides directional migration of cells from all fronts of defect boundary towards the center.
An intensive green emitting terbium complex using a newly designed aromatic hyperbranched polyester as an efficient antenna ligand
Authors: Liu Dan , Zhou Yin-Ning , Zhao Jilian , Xu Yaling , Shen Jingfei , Wu Minggang
Published: October 2017

A Tb3+complex with intensive green luminescence was prepared by using a newly designed aromatic hyperbranched polyester ligand.
Aggregation-induced emission nanofiber as a dual sensor for aromatic amine and acid vapor
Authors: Xue Pengchong , Ding Jipeng , Shen Yanbing , Gao Hongqiang , Zhao Jinyu , Sun Jingbo , Lu Ran
Published: October 2017

An AIE gelator was used as a sensor to quantitatively detect both volatile acid and aromatic amine vapors.
Ratiometric mixed Eu–Tb metal–organic framework as a new cryogenic luminescent thermometer
Authors: N’Dala-Louika Isis , Ananias Duarte , Latouche Camille , Dessapt Rémi , Carlos Luís D. , Serier-Brault Hélène
Published: October 2017

The first example of a dual-emitting luminescent thermometer based on lanthanide isophthalate exhibiting a maximum relative sensitivity of 3.26% K−1, a temperature uncertainty of 0.07 K at 35.5 K, and a repeatability >99.9% between 12 and 230 K.
ITO-free, inkjet-printed transparent organic light-emitting diodes with a single inkjet-printed Al:ZnO:PEI interlayer for sensing applications
Authors: Shu Z. , Beckert E. , Eberhardt R. , Tünnermann A.
Published: October 2017

ITO-free, vacuum-free, inkjet-printed transparent yellow OLEDs with a one-step inkjet-printed Al:ZnO:PEI interlayer.
The super edge connectivity of Kronecker product graphs
Authors: Boruzanli Ekinci Gülnaz , Kirlangic Alpay
Published: October 2017

Let G1 and G2 be two graphs. The Kronecker product G1 × G2 has vertex set V (G1 × G2) = V (G1) × V (G2) and edge set E(G1 × G2) = {(u1, v1)(u2, v2) : u1u2 ∈ E(G1) and v1v2 ∈ E(G2)}. In this paper we determine the super edge–connectivity of G × Kn for n ≥ 3. More precisely, for n ≥ 3, if λ′(G) denotes the super edge–connectivity of G, then at least min{n(n-1)λ′(G), minxy∈E(G){degG(x)+degG(y)}(n-1)-2} edges need to be removed from G × Kn to get a disconnected graph that contains no isolated vertices.
Cirugía de la mano reumática: nuevas expectativas para una vieja conocida
Authors: Montaner-Alonso D. , Novoa-Parra C. , Pérez-Correa J.
Published: October 2017

Es importante que todos los especialistas que tratan a pacientes afectados de artritis reumatoide (AR), conozcan las distintas opciones terapéuticas disponibles, tanto médicas como quirúrgicas, y su manejo en las distintas fases del tratamiento. Nuestro objetivo es actualizar lo que como cirujanos debemos saber sobre el tratamiento médico de la enfermedad, cómo manejar los fármacos durante la programación de la cirugía y cuál es la evidencia sobre las distintas opciones quirúrgicas que podemos barajar ante diversas situaciones originadas por un aparente mismo patrón: la sinovitis.
Cholangiocarcinoma Seeding along a Ventriculoperitoneal Shunt Catheter: A Rare Initial Manifestation
Authors: Morais Barbara , Iglesio Ricardo , Petitto Carlo , Pinto Fernando , Teixeira Manoel
Published: October 2017

Cholangiocarcinoma accounts for less than 2% of all malignant neoplasms. Its cutaneous metastases are extremely rare, accounting for 0.0002% of all cases.

The ventriculoperitoneal (VP) shunt has been considered a potential route for tumor dissemination. This type of tumor proliferation has been well documented in patients with intra-abdominal metastases secondary to brain tumors and, less frequently, brain metastases secondary to intra-abdominal tumors. In spite of that, there are few reports of cutaneous metastases along the VP shunt catheter trajectory.

We present the case of a cholangiocarcinoma cutaneous metastasis at the VP shunt trajectory as the first clinical sign of this type of tumor. The patient had undergone the shunt insertion 4 years earlier due to a hydrocephaly secondary to a subarachnoid hemorrhage. After diagnosis, she was referred to chemotherapy and oncology follow-up.

In the literature, 30 cases of cutaneous metastasis of cholangiocarcinoma were reported. Dissemination has been described involving percutaneous biliary drainage and distant sites. However, the case presented in this study alerts us about the possibility of cutaneous metastasis of intra-abdominal tumors in the trajectory of VP shunt catheters.
Choosing wisely? Multiple Sklerose und laborchemische Suche nach autoimmunen Differenzialdiagnosen – Sind die Leitlinien-Empfehlungen der Deutschen Gesellschaft für Neurologie dazu sinnvoll?
Authors: Becker Jana , Geffken Mareike , Diehl Rolf , Berlit Peter , Krämer Markus
Published: October 2017

Einführung Eine Vielzahl von Erkrankungen können das Bild einer Multiplen Sklerose (MS) imitieren. Bei der Erstdiagnose der MS wird der Ausschluss von Differenzialdiagnosen gefordert. Die Leitlinien der Deutschen Gesellschaft für Neurologie empfehlen die Analyse von 13 obligaten und 9 fakultativen Laborparametern. Zu den obligaten Empfehlungen gehören die Bestimmung der antinukleären Antikörper (ANA) und der Antikörper gegen Doppelstrang-DNS (ds-DNA-AK).

Methodik In der Kohorte einer MS-Ambulanz wurden diejenigen Datensätze ausgewertet, bei denen zumindest teilweise die empfohlenen Laborparameter analysiert wurden. Daneben wurden MRT-Daten, Familienanamnese, klinische Präsentation, evozierte Potenziale und Liquorbefunde erfasst. Prospektiv wurde ein Fragebogen zu differenzialdiagnostisch relevanten rheumatologischen Symptomen eingesetzt.

Ergebnisse Von 554 Patienten, die sich mit Verdacht auf MS in einer MS-Ambulanz vorstellten, waren bei 197 Patienten zumindest teilweise die differenzialdiagnostisch geforderten Laborparameter analysiert worden. Bei 124 Patienten konnte eine MS bestätigt werden. Bei 117 Patienten (59 %) des Gesamtkollektivs mit 197 Patienten waren die ANA mit mindestens einem Titer von 1:80 auffällig. Von den 124 MS-Patienten hatten 68 (55 %) einen positiven ANA-Titer von mindestens 1:80. Weder ANA noch ANCA oder ds-DNA-AK konnten zwischen MS und Nicht-MS unterscheiden. MS-Patienten mit einem positiven ANA-Titer von 1:80 litten häufiger an Autoimmunerkrankungen und hatten eine positive Familienanamnese für MS. Von den 73 Patienten ohne MS waren 49 (67 %) ANA-positiv. In dieser Patientengruppe waren die ANA-positiven Patienten häufiger psychiatrisch erkrankt. Bei den befragten Patienten mit einem ANA-Titer > 1:160 war innerhalb von durchschnittlich 1,9 Jahren keine rheumatologische Erkrankung diagnostiziert worden.

Zusammenfassung In Anlehnung an die amerikanischen „choosing wisely“-Empfehlungen für rheumatologische Erkrankungen erscheint ein obligates breites Laborscreening bei der Erstdiagnose der Multiplen Sklerose ohne konkrete differenzialdiagnostische Verdachtsdiagnose nicht sinnvoll.
Fachtagung Bewegungstherapie 2017
Authors: Brüggemann Silke , Pfeifer Klaus
Published: October 2017

Die Bewegungstherapie nimmt in der medizinischen Rehabilitation der Deutschen Rentenversicherung über alle Indikationen hinweg eine bedeutende Rolle ein. Die Nachhaltigkeit der Bewegungsförderung ist jedoch eine Herausforderung, gilt es doch, die komplexen gesundheitlichen Vorteile von Bewegung auch über die Dauer der Rehabilitation hinaus zugänglich zu machen.
Vorhofflimmern: Diagnostik und was ein Neurologe darüber wissen sollte.
Authors: Rizos Timolaos , Rasch Christina , Horstmann Solveig
Published: October 2017

Vorhofflimmern (VHF) ist ein wichtiger Risikofaktor für Schlaganfälle. Eine orale Antikoagulation kann das Auftreten von Schlaganfällen hocheffektiv verhindern, daher ist die Detektion von VHF besonders wichtig. Insbesondere paroxysmales VHF entgeht den üblichen diagnostischen Methoden häufig. Ein „Goldstandard“ zur VHF Detektion ist nach wie vor nicht etabliert. Grundsätzlich gilt: Je länger die EKG-Ableitung desto höher die zu erreichende Detektionsrate. Neben direkten Nachweisverfahren, existieren indirekte Verfahren wie die Analyse der Herzratenvariabilität und Modelle zur Vorhersage von VHF und zur Selektion von Patienten für erweiterte Detektionsmethoden. Diese sind allerdings bislang noch unzureichend untersucht und werden im Alltag kaum eingesetzt. Konnte VHF nach einem Schlaganfall bzw. einer TIA nachgewiesen werden, besteht eine Indikation für eine orale Antikoagulation. Im Falle positiver Studienergebnisse könnte das Indikationsspektrum einer oralen Antikoagulation in Zukunft auf Patienten mit „embolic stroke of undetermined source“ (ESUS) erweitert werden. Eine mögliche Alternative zur medikamentösen Schlaganfallprävention bei VHF stellt der interventionelle Vorhofohrverschluss dar.
Komplikationsmanagement am oberen GI-Trakt – der Wandel vom Stent zur endoskopischen Vakuumtherapie
Authors: Vowinkel Thorsten , Laukötter Mike , Senninger Norbert , Mennigen Rudolf
Published: October 2017

Das adäquate Komplikationsmanagement am oberen Gastrointestinal (GI)-trakt ist für Patienten mit spontanen oder iatrogenen Perforationen der Speiseröhre oder postoperativ nach Ösophagusresektionen entscheidend für ihr Überleben. Neben den beiden extremen Therapieformen, wie der konservativen Therapie auf der einen Seite und einer operativen, zumeist resezierenden Therapie, auf der anderen Seite, haben sich in den letzten Jahren zunehmend minimalinvasive endoskopische Therapiemöglichkeiten etabliert. Lange Zeit galt die Implantation eines selbstexpandierenden Ösophagusstents als Goldstandard im endoskopischen Komplikationsmanagement am oberen GI-Trakt. Daneben hat sich die endoskopische Vakuumtherapie (EVT) durch überzeugende Heilungsraten sowie geringe Komplikationen in vielen Kliniken zunehmend fest etabliert und wird im Komplikationsmanagement am oberen GI-Trakt mittlerweile immer öfter als Therapie der ersten Wahl mit sehr gutem Erfolg eingesetzt. Der Vorteil der EVT gegenüber der Stenttherapie liegt in der Förderung der Wundheilung durch den negativen Sog, den Abtransport von Sekreten und der damit verbesserten Sepsiskontrolle.
Rehabilitation eines Patienten mit Contergan-Dysmelie und seine orthopädietechnische Versorgung
Authors: Hesselschwerdt Hans-Jürgen , Spiekerkötter Thorsten
Published: October 2017

Wenn ein optimales Reha-Angebot auf einen willensstarken Patienten trifft, sind erstaunliche Ergebnisse möglich. Im hier geschilderten Fall kam der Patient mit angeborener Tetraphokomelie, der nach einer Schulter-OP seit 3 Jahren auf einen Rollstuhl angewiesen war, in die Theresienklinik Bad Krozingen. Mithilfe einer individuellen Einzeltherapie mit Physio- und Ergotherapeuten konnte der Patient nach wenigen Wochen wieder mit 2 Gehstöcken gehen.
Study of interactions between metal ions and protein model compounds by energy decomposition analyses and the AMOEBA force field
Authors: Jing Zhifeng , Qi Rui , Liu Chengwen , Ren Pengyu
Published: October 2017

The interactions between metal ions and proteins are ubiquitous in biology. The selective binding of metal ions has a variety of regulatory functions. Therefore, there is a need to understand the mechanism of protein-ion binding. The interactions involving metal ions are complicated in nature, where short-range charge-penetration, charge transfer, polarization, and many-body effects all contribute significantly, and a quantitative description of all these interactions is lacking. In addition, it is unclear how well current polarizable force fields can capture these energy terms and whether these polarization models are good enough to describe the many-body effects. In this work, two energy decomposition methods, absolutely localized molecular orbitals and symmetry-adapted perturbation theory, were utilized to study the interactions between Mg2+/Ca2+ and model compounds for amino acids. Comparison of individual interaction components revealed that while there are significant charge-penetration and charge-transfer effects in Ca complexes, these effects can be captured by the van der Waals (vdW) term in the AMOEBA force field. The electrostatic interaction in Mg complexes is well described by AMOEBA since the charge penetration is small, but the distance-dependent polarization energy is problematic. Many-body effects were shown to be important for protein-ion binding. In the absence of many-body effects, highly charged binding pockets will be over-stabilized, and the pockets will always favor Mg and thus lose selectivity. Therefore, many-body effects must be incorporated in the force field in order to predict the structure and energetics of metalloproteins. Also, the many-body effects of charge transfer in Ca complexes were found to be non-negligible. The absorption of charge-transfer energy into the additive vdW term was a main source of error for the AMOEBA many-body interaction energies.
Spatial confinement effect on femtosecond laser-induced Cu plasma spectroscopy
Authors: Wang Xiaowei , Chen Anmin , Wang Ying , Zhang Dan , Sui Laizhi , Ke Da , Li Suyu , Jiang Yuanfei , Jin Mingxing
Published: October 2017

The effect of spatial confinement on femtosecond laser-induced Cu plasmas was investigated by time-resolved spectroscopy. The cylindrical cavities with various diameters (2 and 3 mm) and various heights (2, 3, and 4 mm) were placed on the sample surface. An obvious enhancement in the emission intensity of Cu atomic lines was observed when a cylindrical cavity was used to confine the femtosecond laser-induced Cu plasmas. The results showed that enhancement ratios in femtosecond laser-induced breakdown spectroscopy with spatial confinement varied with cavity diameters and atomic emission lines selected. The spatial confinement effect was not significantly influenced by the cavity height because the height of plasma plume is lower than the cavity height. The maximum enhancement ratio for the emission intensities of the Cu atomic lines was measured to be around 3 at a time delay of 3.5 μs when the cavity diameter is 2 mm. The spectral enhancement is attributed to the compression of the plasma by the reflected shock wave.
Reconfigurable origami sonic barriers with tunable bandgaps for traffic noise mitigation
Authors: Thota M. , Wang K. W.
Published: October 2017

An origami sonic barrier composed of cylindrical inclusions attached onto an origami sheet is proposed. The idea allows for tunable sound blocking properties for application in attenuating complex traffic noise spectra. Folding of the underlying origami sheet transforms the periodicity of the inclusions between different Bravais lattices, viz. between a square and a hexagonal lattice, and such significant lattice re-configuration leads to drastic tuning of dispersion characteristics. The wave tuning capabilities are corroborated via performing theoretical and numerical investigations using a plane wave expansion method and an acoustic simulation package of COMSOL, while experiments are performed on a one-seventh scaled-down model of origami sonic barrier to demonstrate the lattice re-configuration between different Bravais lattices and the associated bandgap adaptability. Good sound blocking performance in the frequency range of traffic noise spectra combined with less efforts, required for actuating one-degree of freedom folding mechanism, makes the origami sonic barrier a potential candidate for mitigating complex traffic noise.
Navier slip model of drag reduction by Leidenfrost vapor layers
Authors: Berry Joseph D. , Vakarelski Ivan U. , Chan Derek Y. C. , Thoroddsen Sigurdur T.
Published: October 2017

Recent experiments found that a hot solid sphere that is able to sustain a stable Leidenfrost vapor layer in a liquid exhibits significant drag reduction during free fall. The variation of the drag coefficient with Reynolds number deviates substantially from the characteristic drag crisis behavior at high Reynolds numbers. Measurements based on liquids of different viscosities show that the onset of the drag crisis depends on the viscosity ratio of the vapor to the liquid. Here we attempt to characterize the complexity of the Leidenfrost vapor layer with respect to its variable thickness and possible vapor circulation within, in terms of the Navier slip model that is defined by a slip length. Such a model can facilitate tangential flow and thereby alter the behavior of the boundary layer. Direct numerical and large eddy simulations of flow past a sphere at moderate to high Reynolds numbers (102≤Re≤4×104) are employed to quantify comparisons with experimental results, including the drag coefficient and the form of the downstream wake on the sphere. This provides a simple one parameter characterization of the drag reduction phenomenon due to a stable vapor layer that envelops a solid body.