bluuebunny/crossref_metadata_2025 huggingface dataset:
Interventional Cardio-Oncology: Unique Challenges and Considerations in a High-Risk Population
Authors: Leiva Orly , Alam Usman , Bohart Isaac , Yang Eric H.
Published: June 2023

Opinion Statement

Patients with cancer are at risk of developing cardiovascular disease (CVD) including atherosclerotic heart disease (AHD), valvular heart disease (VHD), and atrial fibrillation (AF). Advances in percutaneous catheter-based treatments, including percutaneous coronary intervention (PCI) for AHD, percutaneous valve replacement or repair for VHD, and ablation and left atrial appendage occlusion devices (LAAODs) for AF, have provided patients with CVD significant benefit in the recent decades. However, trials and registries investigating outcomes of these procedures often exclude patients with cancer. As a result, patients with cancer are less likely to undergo these therapies despite their benefits. Despite the inclusion of cancer patients in randomized clinical trial data, studies suggest that cancer patients derive similar benefits of percutaneous therapies for CVD compared with patients without cancer. Therefore, percutaneous interventions for CVD should not be withheld in patients with cancer, as they may still benefit from these procedures.
‘Blowjobs are Jobs Too’: An Exploratory Study into Victimization Experiences of Male Sex Workers in Botswana
Authors: Mashumba Lesedi
Published: June 2023

This study conducted in-depth face to face interviews with twenty male sex workers (MSWs) servicing sex tourists in Botswana. Expert opinions were also sought with support organizations and the police in Botswana to understand more about male sex work in Botswana. The research questions were directed at exploring (a) how male sex workers experienced and perceived victimisation in their interactions with sex tourists, (b) what role support groups played, and (c) what reforms both male sex workers and support groups propose for the male sex work industry. The article presents their victimisation experiences, health risks and opinions of the support groups. The study found out that MSWs frequently experienced physical violence and sexual assaults such as stealthing, especially male sex workers who identified as gay or bisexual. MSWs never reported their victimisation experiences to the police due to fear of stigmatisation, shaming and possible arrests given the partial criminalisation of sex work. Support groups on the other, as non-governmental organizations offered the much-needed support services such as distribution of lubes, condoms, HIV screening, counselling, and ARV and PrEP access and management for sex workers. This study contributes to the limited literature examining the experiences of MSWs, demonstrating their vulnerability and the need for interventions to mitigate their victimisation and protect their health. Many sex workers emphasised constitutional protections of their rights, the need to decriminalise, as well as the promotion of safety and security. The study argues that traditionally, culture and religion have produced a gender-biased socio-cultural parameters that place males as providers, hence, their vulnerability to victimisation and sexual violence is often unnoticed, which also makes reporting difficult to law enforcement.
When push comes to shove - RNA polymerase and DNA-bound protein roadblocks
Authors: Hao Nan , Donnelly Alana J. , Dodd Ian B. , Shearwin Keith E.
Published: June 2023

In recent years, transcriptional roadblocking has emerged as a crucial regulatory mechanism in gene expression, whereby other DNA-bound obstacles can block the progression of transcribing RNA polymerase (RNAP), leading to RNAP pausing and ultimately dissociation from the DNA template. In this review, we discuss the mechanisms by which transcriptional roadblocks can impede RNAP progression, as well as how RNAP can overcome these obstacles to continue transcription. We examine different DNA-binding proteins involved in transcriptional roadblocking and their biophysical properties that determine their effectiveness in blocking RNAP progression. The catalytically dead CRISPR-Cas (dCas) protein is used as an example of an engineered programmable roadblock, and the current literature in understanding the polarity of dCas roadblocking is also discussed. Finally, we delve into a stochastic model of transcriptional roadblocking and highlight the importance of transcription factor binding kinetics and its resistance to dislodgement by an elongating RNAP in determining the strength of a roadblock.
Requesting HIV Results Be Conveyed in-Person: Perspectives of Clinicians and People Recently Diagnosed with HIV
Authors: Wells Nathanael , Murphy Dean , Ellard Jeanne , Howard Chris , Keen Phillip , Fairley Christopher , Donovan Basil , Prestage Garrett ,   on behalf of the RISE Study Team
Published: June 2023

Introduction

Guidelines recommend that, where possible, clinicians convey HIV-positive test results in person in Australia. However, HIV-negative and all other STI results are routinely delivered by phone or text message. Requesting individuals to obtain positive HIV test results in person could be a deviation from the standard delivery of healthcare and be interpreted as indicating a positive HIV diagnosis.

Methods

This paper is based on two related, ongoing qualitative studies conducted in Australia with HIV healthcare providers and people recently diagnosed with HIV. In study one, in-depth, semi-structured interviews were conducted with people who had recently received a positive HIV diagnosis. In study two, in-depth, semi-structured interviews were conducted with HIV healthcare and peer support providers. Interviews were analyzed thematically.

Results

While clinicians were willing to convey HIV-positive diagnoses by phone, most preferred in-person delivery. In-person delivery enabled clinicians to assess visual cues to better respond to the psychological and emotional needs of patients. For some participants living with HIV, however, the requirement to return to the clinic was interpreted as an unofficial HIV-positive diagnosis. This led to a period in which recently diagnosed participants believed they were HIV-positive without having received an explicit diagnosis.

Conclusion

Protocols for delivering HIV diagnoses by phone, followed by a face-to-face appointment, may reduce the period of anxiety for some patients and assist with an early connection to HIV care and support.

Policy Implications

In some instances, conveying HIV diagnoses by phone may be more appropriate than recalling individuals to the clinic to deliver a positive HIV diagnosis in person.
I Am More than HIV: A Qualitative Exploration of Factors That Can Strengthen Resilience Among HIV-Positive Gay Men in Australia
Authors: Lucas Neil A. , Bates Glen W. , Buzwell Simone
Published: June 2023

Introduction

HIV-positive gay men continue to experience stigma related to sexual orientation and HIV status. Although resilience toward such stressors can be achieved, limited Australian research exists that examines how resilience is strengthened toward these dual stigmas.

Methods

A total of 20 men from Melbourne, Australia, participated in semi-structured interviews between March and October 2019 to explore ways in which they manage such stigmas.

Results

Reflexive thematic analysis identified two primary themes: (1) “intrapersonal control,” which relates to individual mind set and lifestyle changes that participants utilized to strengthen resilience; (2) “systemic change,” which includes participants’ needs for better public health messaging. Findings show resilience was enhanced when proactive approaches to sexual orientation, HIV health appraisal, lifestyle changes, and social support were made. Further, outdated HIV awareness campaigns and a lack of current messaging regarding HIV transmission in the wider community were identified as inhibiting resilience development and promoting stigma among gay men.

Conclusion

The results from this study show ways that Australian gay men strengthen their resilience through both intrapersonal (e.g., self-awareness, reappraisal, and self-efficacy) and external resources (e.g., education and public awareness) and how health care providers and social policy makers could better support the men to achieve this.

Social-Policy Implications

Findings suggest that targeted public health responses are required to compliment the advances made in biomedicine and viral suppression.
Transcriptome analysis reveals genes connected to temperature adaptation in juvenile antarctic krill Euphausia superba
Authors: Liu Yongliang , Li Lingzhi , Yang Jialiang , Huang Hongliang , Song Wei
Published: June 2023

Background

The Antarctic krill, Euphausia superba (E. superba), is a key organism in the Antarctic marine ecosystem and has been widely studied. However, there is a lack of transcriptome data focusing on temperature responses.

Methods

In this study, we performed transcriptome sequencing of E. superba samples exposed to three different temperatures: −1.19 °C (low temperature, LT), − 0.37 °C (medium temperature, MT), and 3 °C (high temperature, HT).

Results

Illumina sequencing generated 772,109,224 clean reads from the three temperature groups. In total, 1,623, 142, and 842 genes were differentially expressed in MT versus LT, HT versus LT, and HT versus MT, respectively. Moreover, Kyoto Encyclopedia of Genes and Genomes analysis revealed that these differentially expressed genes were mainly involved in the Hippo signaling pathway, MAPK signaling pathway, and Toll−like receptor signaling pathway. Quantitative reverse-transcription PCR revealed that ESG037073 expression was significantly upregulated in the MT group compared with the LT group, and ESG037998 expression was significantly higher in the HT group than in the LT group.

Conclusions

This is the first transcriptome analysis of E. superba exposed to three different temperatures. Our results provide valuable resources for further studies on the molecular mechanisms underlying temperature adaptation in E. superba.
Dairy and Plant-Based Milks: Implications for Nutrition and Planetary Health
Authors: Ramsing Rebecca , Santo Raychel , Kim Brent F. , Altema-Johnson Daphene , Wooden Alyssa , Chang Kenjin B. , Semba Richard D. , Love David C.
Published: June 2023

Purpose of Review

Dairy milk products are dominant in the market; however, plant-based milks are gaining prominence among USA consumers. Many questions remain about how plant-based milk products compare to dairy milk from a nutrition, public health, and planetary health perspective. Here, we compare the retail sales, nutrient profiles, and known health and environmental impacts of the production and consumption of dairy and plant-based milks and identify knowledge gaps for future studies. For our plant-based milk comparisons, we reviewed almond, soy, oat, coconut, rice, pea, cashew, and other plant-based milks as data were available.

Recent Findings

The retail unit price of plant-based milks was generally higher than that of cow’s milk, making it less accessible to lower-income groups. Many plant-based milks are fortified to match the micronutrient profile of dairy milk more closely. Notable differences remained, especially in protein, zinc, and potassium, depending on the base ingredient and individual product. Some plant-based milks contain added sugar to improve flavor. Plant-based milks were generally associated with lower environmental impacts (e.g., greenhouse gas emissions, water use) than cow’s milk, with the notable exception of the higher water footprint of almond milk.

This review of recent studies and consumer purchases confirmed that retail sales of plant-based milks are increasing and shifting among products. Further research is needed to better characterize the environmental impacts of newer plant-based milks, such as cashew, hemp, and pea milks; consumer attitudes and behavior towards plant-based milks; and the safety and potential health effects related to their long-term and more frequent consumption.
The causal nexus between bank indices and geopolitical risk: bootstrap causality analysis under horizontal sector dependence
Authors: Yıldırım Hakan , Şahin Eyyüp Ensari , Akdağ Saffet , Alola Andrew Adewale
Published: June 2023

As the global economy thrives and pushes for sustainable growth, there are also a plethora of non-economic challenges arising from the respective dimensions of insecurity and geopolitical tensions such as inter- and intra-country conflicts. Geopolitical risk mostly arises from security tensions, war, and terrorist incidents, which hamper peaceful inter-country and regional cooperation, thus endangering state institutions such as financial institutions. Given this observation, the current study examines the relationship between the geopolitical risk index and bank indices by employing the bootstrap panel causality approach over a monthly period from September 2003 to December 2018. In this case, the causality analysis of the geopolitical risk index and bank indices for six (6) countries (China, Indonesia, Israel, Philippines, Saudi Arabia, and Turkey) was performed. Importantly, the investigation found causality only from the geopolitical risk index to bank indices in Turkey and Israel. Given the statistical evidence from the study, we offer related policy recommendations, especially for the examined countries.
Assessment of the Impact of the Rotavirus Vaccine Against Severe Rotaviral Diarrhea in Uzbekistan
Authors: Musabaev Erkin , Ismailov Umed , Ibadullaeva Nargiz , Khikmatullaeva Aziza , Norbaev Ilham , Primov Umar , Ahrarova Dilorom , Sharapov Saidkhon , Yusupov Ulugbek , Latipov Renat
Published: June 2023

Background

This article presents the results of a long-term study of the impact of rotavirus vaccination in Uzbekistan. Uzbekistan is the first country in the Central Asian region to introduce rotavirus vaccination into the national compulsory vaccination calendar. The study aimed to evaluate the impact of rotavirus vaccination on hospitalizations due to all-cause AGE and RVGE in children < 5 years of age in Uzbekistan.

Methods

Detection of rotavirus antigen was performed using Rotavirus-Antigen-IFA-BEST "Vector Best" kit (Novosibirsk, Russia).

Results

The total of 20,128 children under 5 years of age were hospitalized in sentinel hospitals with a diagnosis of acute gastroenteritis during the study period (2019–2020). Of this number of children, 4481 children (22.2%) were included in the study. Of 4481 children, 367 (8.2%) children tested positive for rotavirus. In our study, decrease in the rotavirus rate was noted in all age groups. The peak of rotavirus positivity occurred in the months of January and February.

Conclusion

The average rotavirus-positive rate in the period (2019–2020) was 8.2% and the absolute percentage decrease was 18.1% compared to the pre-vaccination period (2005–2009) where the rotavirus-positive rate was 26.3%. The percentage of prevented cases averaged 68.8%.
The Medieval Changeling
Authors: Sawyer Rose A.
Published: June 2023

The first comprehensive study of medieval changelings and associated attitudes to the health and care of children in the period.
Enhanced Continental Weathering as a Trigger for the End‐Devonian Hangenberg Crisis
Authors: Qie Wenkun , Zhang Junpeng , Luo Genming , Algeo Thomas J. , Chen Bo , Xiang Lei , Liang Kun , Liu Xianyi , Pogge von Strandmann Philip A. E. , Chen Jitao , Wang Xiangdong
Published: June 2023

The Hangenberg Crisis coincided with a large decline of biodiversity and widespread anoxia in the end‐Devonian ocean. Previous research attributed marine anoxia to the spread of deeply‐rooted plants and/or increased volcanism on the continents, but crucial links have not been thoroughly explored. Herein, we propose enhanced weathering as a key trigger, as evidenced by a negative shift (∼8‰) in lithium isotopes and a coupled response in carbon isotopes of marine carbonates in South China. Our findings imply that rapid weathering of crustal rocks increased nutrient delivery to the ocean, as indicated by an increase in the carbonate‐associated phosphate levels, contributing to oceanic eutrophication. In the absence of massive volcanic emissions and intense orogeny, the cause of enhanced continental weathering was likely the expansion of the terrestrial rhizosphere, highlighting the potential for land plant evolution to initiate weathering changes of sufficient severity to trigger a major bio/environmental crisis in the Earth system.
Influence of Land–Atmosphere Coupling on Low Temperature Extremes Over Southern Eurasia
Authors: Qi Yajing , Chen Haishan , Zhu Siguang
Published: June 2023

Land–atmosphere coupling is a crucial factor influencing extreme weather and climate. Based on the ERA5‐Land reanalysis data set and sensitivity experiments with the CESM1.2.2 model, this study investigated the possible impacts of land–atmosphere coupling on low temperature extremes (LTEs) during fall and winter over southern Eurasia (SEA). The results suggested that LTEs show evident interdecadal variations in recent 60 years (1958–2017) and exhibit significant positive correlations with soil moisture anomalies based on the ERA5‐Land data set. Numerical simulations (the control run and sensitivity run with prescribed climatological soil moisture) isolated the effects of soil moisture‐related land surface processes and demonstrated that land–atmosphere coupling explains around 70% of temperature variability, increases the near‐surface air temperature by 0.81 K, and favors the reduction of LTEs by 8.20 d·yr−1. In the perturbation scenarios, the impacts of coupling on the frequency and spatial coverage of LTEs are more prominent. Further analysis revealed that the thermal cooling anomalies induced by the coupling could alter the atmospheric circulation, thereby affecting the frequency and intensity of LTEs. When the land–atmosphere interactions are weakened, the surface latent heat flux increases accompanied by intense evapotranspiration, while the sensible heat flux and net infrared radiation decrease. Land surface cooling is apparent, and it weakens with increasing altitude, along with geopotential height anomalies that are positive in the lower troposphere and weaker at higher altitudes. The northeasterly wind anomalies over the East European Plain facilitate the development of cold advection, providing a favorable circulation background for the generation of LTEs over SEA.
Enhanced Influence of Tropical Pacific Sea Surface Temperature Anomalies on Spring Extreme Heat Events Over Mid‐High Latitude Eurasia
Authors: Liu Sichang , Sun Jianqi
Published: June 2023

This study presents an interdecadal enhancement in the interannual relationship between spring extreme heat events (EHEs) over mid‐high latitude Eurasia and winter sea surface temperature (SST) over the tropical central‐eastern Pacific (TECP) after the late 1990s. Comparative analyses of the two subperiods before and after the late 1990s show that the interdecadal changes in the winter TECP SST could be responsible for such changeable relationship. After the late 1990s, the winter TECP SST anomalies (SSTA) feature a central Pacific (CP)‐type pattern. The CP‐type SSTA cause an anticyclone anomaly over the North Atlantic through the Pacific‐North American‐like teleconnection, further leading to a dipole SST pattern over the tropical to mid‐latitude North Atlantic. The CP‐type SSTA and North Atlantic dipole SSTA persist well from winter to spring, and the North Atlantic dipole SSTA favor the propagation of the CP‐type SST‐excited Rossby wave train to Eurasia in spring. Therefore, the TCEP SSTA have a significant interannual relationship with the spring Eurasian atmospheric circulations and EHEs after the late 1990s. The above physical processes are further confirmed by the SSTA sensitivity numerical experiments. In contrast, before the late 1990s, the winter TECP SSTA show an eastern Paciﬁc‐type pattern, which is related to weak and insignificant North Atlantic SSTA and spring Eurasian atmospheric circulations; consequently, the TCEP SSTA have a weak relationship with the EHEs variations during this period. The TCEP SSTA with one season leading can provide valuable information for the prediction of spring Eurasian EHEs variations in the recent two decades.
Saturn’s Shiny Rings May Be Pretty Young
Authors: Cartier Kimberly
Published: June 2023

The rings are fairly shiny despite being bombarded by dust, indicating that they haven’t been around for very long.
Utilizing Helium Ion Chemistry to Derive Mixing Ratios of Heavier Neutral Species in Saturn's Equatorial Ionosphere
Authors: Dreyer Joshua , Vigren Erik , Johansson Fredrik L. , Waite J. Hunter
Published: June 2023

A surprisingly strong influx of organic‐rich material into Saturn's upper atmosphere from its rings was observed during the proximal obits of the Grand Finale of the Cassini mission. Measurements by the Ion and Neutral Mass Spectrometer (INMS) gave insights into the composition of the material, but it remains to be resolved what fraction of the inferred heavy volatiles should be attributed as originating from the fragmentation of dust particles in the instrument versus natural ablation of grains in the atmosphere. In the present study, we utilize measured light ion and neutral densities to further constrain the abundances of heavy volatiles in Saturn's ionosphere through a steady‐state model focusing on helium ion chemistry. We first show that the principal loss mechanism of He+ in Saturn's equatorial ionosphere is through reactions with species other than H2. Based on the assumption of photochemical equilibrium at altitudes below 2,500 km, we then proceed by estimating the mixing ratio of heavier volatiles down to the closest approaches for Cassini's proximal orbits 288 and 292. Our derived mixing ratios for the inbound part of both orbits fall below those reported from direct measurements by the INMS, with values of ∼2 × 10−4 at closest approaches and order‐of‐magnitude variations in either direction over the orbits. This aligns with previous suggestions that a large fraction of the neutrals measured by the INMS stems from the fragmentation of infalling dust particles that do not significantly ablate in the considered part of Saturn's atmosphere and are thus unavailable for reactions.
Temporal Variability of Fluvial Sand Composition: An Annual Time Series From Four Rivers in SW Germany
Authors: Stutenbecker L. , Scheuvens D. , Hinderer M. , Hornung J. , Petschick R. , Raila N. , Schwind E.
Published: June 2023

The sampling of fluvial sediment is subject to many sources of uncertainty, for example, time and location, and the number of samples collected. It is nevertheless commonly assumed that a sample taken at one time and location provides a somewhat averaged compositional signal. Any spatial or temporal variability of this signal is often neglected. This study investigates how the composition of bed load sand changes over an observation period of 1 year in four river basins with differing bedrock geology in southwestern Germany. Up to 12 bulk sediment samples were taken at the same locations using the same approach and analyzed for their granulometry and geochemistry. The results indicate that (a) different grain sizes yield different compositions due to source rock composition and hydraulic sorting effects, (b) bulk sediment composition changes temporally due to changing grain‐size distribution, and (c) compared to the bulk sample, the composition of narrow grain sizes is temporally more stable but nevertheless has an average variability of 15%. Because heavy mineral‐bound elements such as Zr have the highest variability, we relate a major component of compositional variability to temporally varying heavy mineral concentrations in response to hydrodynamic processes. Mixing modeling demonstrates that the fluvial sand faithfully reflects its catchment geology and that the sediment sources do not change substantially during the observation period, even during a flooding event. We conclude (a) that the causes for compositional variability may be disentangled using chemical and granulometric time series data and (b) that narrow grain sizes yield representative source rock contributions.
The effect of ketamine on synaptic mistuning induced by impaired glutamate reuptake
Authors: Vazquez-Juarez Erika , Srivastava Ipsit , Lindskog Maria
Published: June 2023

Mistuning of synaptic transmission has been proposed to underlie many psychiatric disorders, with decreased reuptake of the excitatory neurotransmitter glutamate as one contributing factor. Synaptic tuning occurs through several diverging and converging forms of plasticity. By recording evoked field postsynaptic potentials in the CA1 area in hippocampal slices, we found that inhibiting glutamate transporters using DL-TBOA causes retuning of synaptic transmission, resulting in a new steady state with reduced synaptic strength and a lower threshold for inducing long-term synaptic potentiation (LTP). Moreover, a similar reduced threshold for LTP was observed in a rat model of depression with decreased levels of glutamate transporters. Most importantly, we found that the antidepressant ketamine counteracts the effects of increased glutamate on the various steps involved in synaptic retuning. We, therefore, propose that ketamine’s mechanism of action as an antidepressant is to restore adequate synaptic tuning.
Metabolomics signatures of depression: the role of symptom profiles
Authors: de Kluiver Hilde , Jansen Rick , Penninx Brenda W. J. H. , Giltay Erik J. , Schoevers Robert A. , Milaneschi Yuri
Published: June 2023

Depression shows a metabolomic signature overlapping with that of cardiometabolic conditions. Whether this signature is linked to specific depression profiles remains undetermined. Previous research suggested that metabolic alterations cluster more consistently with depressive symptoms of the atypical spectrum related to energy alterations, such as hyperphagia, weight gain, hypersomnia, fatigue and leaden paralysis. We characterized the metabolomic signature of an “atypical/energy-related” symptom (AES) profile and evaluated its specificity and consistency. Fifty-one metabolites measured using the Nightingale platform in 2876 participants from the Netherlands Study of Depression and Anxiety were analyzed. An ‘AES profile’ score was based on five items of the Inventory of Depressive Symptomatology (IDS) questionnaire. The AES profile was significantly associated with 31 metabolites including higher glycoprotein acetyls (β = 0.13, p = 1.35*10-12), isoleucine (β = 0.13, p = 1.45*10-10), very-low-density lipoproteins cholesterol (β = 0.11, p = 6.19*10-9) and saturated fatty acid levels (β = 0.09, p = 3.68*10-10), and lower high-density lipoproteins cholesterol (β = −0.07, p = 1.14*10-4). The metabolites were not significantly associated with a summary score of all other IDS items not included in the AES profile. Twenty-five AES-metabolites associations were internally replicated using data from the same subjects (N = 2015) collected at 6-year follow-up. We identified a specific metabolomic signature—commonly linked to cardiometabolic disorders—associated with a depression profile characterized by atypical, energy-related symptoms. The specific clustering of a metabolomic signature with a clinical profile identifies a more homogenous subgroup of depressed patients at higher cardiometabolic risk, and may represent a valuable target for interventions aiming at reducing depression’s detrimental impact on health.
Wear effects between polymethyl methacrylate occlusal splints and opposing dentin surfaces during bruxism mimicking events
Authors: Potewiratnanond Prangtip , Ekrojanakul Cholakorn , Harikul Tatcharee , Kositvanich Rapatporn
Published: June 2023

Objectives

To compare the surface wear rate between polymethyl methacrylate (PMMA)-based occlusal splints and opposing dentin-exposed teeth in bruxism simulating models.

Materials and methods

PMMA-based occlusal splints and extracted premolars were tested on a chewing stimulator at 30,000 or 60,000 cycles. Dentin wear was measured under a stereomicroscope and PMMA wear was determined with an optical profilometer. In addition, wear surface topography was assessed and quantified by scanning electron microscopy (SEM).

Results

Wear rate of PMMA was significantly greater (11 times) than that of dentin specimens at 60,000 cycles, though these findings were not observed at 30,000 cycles. When comparing wear rates within each group at different duration cycles, PMMA surfaces exhibited an average wear rate 1.4 times higher with high duration cycles, while dentin surfaces displayed a marginal decrease in wear. In SEM micrographs, PMMA surfaces displayed more wear abrasion lines with higher duration cycles. However, dentin surfaces did not exhibit major differences between low and high duration cycles.

Conclusion

Wear rate on PMMA-based occlusal splints remarkably increases upon high chewing cycles mimicking bruxism comparing with the rate on dentin. Hence, it is reasonable for bruxing patients to wear single-arch PMMA-based occlusal splints to protect opposing dentin-exposed teeth.
Global warming accelerates soil heterotrophic respiration
Authors: Nissan Alon , Alcolombri Uria , Peleg Nadav , Galili Nir , Jimenez-Martinez Joaquin , Molnar Peter , Holzner Markus
Published: June 2023

Carbon efflux from soils is the largest terrestrial carbon source to the atmosphere, yet it is still one of the most uncertain fluxes in the Earth’s carbon budget. A dominant component of this flux is heterotrophic respiration, influenced by several environmental factors, most notably soil temperature and moisture. Here, we develop a mechanistic model from micro to global scale to explore how changes in soil water content and temperature affect soil heterotrophic respiration. Simulations, laboratory measurements, and field observations validate the new approach. Estimates from the model show that heterotrophic respiration has been increasing since the 1980s at a rate of about 2% per decade globally. Using future projections of surface temperature and soil moisture, the model predicts a global increase of about 40% in heterotrophic respiration by the end of the century under the worst-case emission scenario, where the Arctic region is expected to experience a more than two-fold increase, driven primarily by declining soil moisture rather than temperature increase.
A flavin-monooxygenase catalyzing oxepinone formation and the complete biosynthesis of vibralactone
Authors: Feng Ke-Na , Zhang Yue , Zhang Mingfang , Yang Yan-Long , Liu Ji-Kai , Pan Lifeng , Zeng Ying
Published: June 2023

Oxepinone rings represent one of structurally unusual motifs of natural products and the biosynthesis of oxepinones is not fully understood. 1,5-Seco-vibralactone (3) features an oxepinone motif and is a stable metabolite isolated from mycelial cultures of the mushroomBoreostereum vibrans. Cyclization of3forms vibralactone (1) whose β-lactone-fused bicyclic core originates from 4-hydroxybenzoate, yet it remains elusive how 4-hydroxybenzoate is converted to3especially for the oxepinone ring construction in the biosynthesis of1. In this work, using activity-guided fractionation together with proteomic analyses, we identify an NADPH/FAD-dependent monooxygenase VibO as the key enzyme performing a crucial ring-expansive oxygenation on the phenol ring to generate the oxepin-2-one structure of3. The crystal structure of VibO reveals that it forms a dimeric phenol hydroxylase-like architecture featured with a unique substrate-binding pocket adjacent to the bound FAD. Computational modeling and solution studies provide insight into the likely VibO active site geometry, and suggest possible involvement of a flavin-C4a-OO(H) intermediate.
Solution-processable polymers of intrinsic microporosity for gas-phase carbon dioxide photoreduction
Authors: Moruzzi Floriana , Zhang Weimin , Purushothaman Balaji , Gonzalez-Carrero Soranyel , Aitchison Catherine M. , Willner Benjamin , Ceugniet Fabien , Lin Yuanbao , Kosco Jan , Chen Hu , Tian Junfu , Alsufyani Maryam , Gibson Joshua S. , Rattner Ed , Baghdadi Yasmine , Eslava Salvador , Neophytou Marios , Durrant James R. , Steier Ludmilla , McCulloch Iain
Published: June 2023

Four solution-processable, linear conjugated polymers of intrinsic porosity are synthesised and tested for gas phase carbon dioxide photoreduction. The polymers’ photoreduction efficiency is investigated as a function of their porosity, optical properties, energy levels and photoluminescence. All polymers successfully form carbon monoxide as the main product, without the addition of metal co-catalysts. The best performing single component polymer yields a rate of 66 μmol h−1 m−2, which we attribute to the polymer exhibiting macroporosity and the longest exciton lifetimes. The addition of copper iodide, as a source of a copper co-catalyst in the polymers shows an increase in rate, with the best performing polymer achieving a rate of 175 μmol h−1 m−2. The polymers are active for over 100 h under operating conditions. This work shows the potential of processable polymers of intrinsic porosity for use in the gas phase photoreduction of carbon dioxide towards solar fuels.
Splicing activates transcription from weak promoters upstream of alternative exons
Authors: Uriostegui-Arcos Maritere , Mick Steven T. , Shi Zhuo , Rahman Rufuto , Fiszbein Ana
Published: June 2023

Transcription and splicing are intrinsically coupled. Alternative splicing of internal exons can fine-tune gene expression through a recently described phenomenon called exon-mediated activation of transcription starts (EMATS). However, the association of this phenomenon with human diseases remains unknown. Here, we develop a strategy to activate gene expression through EMATS and demonstrate its potential for treatment of genetic diseases caused by loss of expression of essential genes. We first identified a catalog of human EMATS genes and provide a list of their pathological variants. To test if EMATS can be used to activate gene expression, we constructed stable cell lines expressing a splicing reporter based on the alternative splicing of motor neuron 2 (SMN2) gene. Using small molecules and antisense oligonucleotides (ASOs) currently used for treatment of spinal muscular atrophy, we demonstrated that increase of inclusion of alternative exons can trigger an activation of gene expression up to 45-fold by enhancing transcription in EMATS-like genes. We observed the strongest effects in genes under the regulation of weak human promoters located proximal to highly included skipped exons.
Experimental demonstration of a skyrmion-enhanced strain-mediated physical reservoir computing system
Authors: Sun Yiming , Lin Tao , Lei Na , Chen Xing , Kang Wang , Zhao Zhiyuan , Wei Dahai , Chen Chao , Pang Simin , Hu Linglong , Yang Liu , Dong Enxuan , Zhao Li , Liu Lei , Yuan Zhe , Ullrich Aladin , Back Christian H. , Zhang Jun , Pan Dong , Zhao Jianhua , Feng Ming , Fert Albert , Zhao Weisheng
Published: June 2023

Physical reservoirs holding intrinsic nonlinearity, high dimensionality, and memory effects have attracted considerable interest regarding solving complex tasks efficiently. Particularly, spintronic and strain-mediated electronic physical reservoirs are appealing due to their high speed, multi-parameter fusion and low power consumption. Here, we experimentally realize a skyrmion-enhanced strain-mediated physical reservoir in a multiferroic heterostructure of Pt/Co/Gd multilayers on (001)-oriented 0.7PbMg1/3Nb2/3O3−0.3PbTiO3 (PMN-PT). The enhancement is coming from the fusion of magnetic skyrmions and electro resistivity tuned by strain simultaneously. The functionality of the strain-mediated RC system is successfully achieved via a sequential waveform classification task with the recognition rate of 99.3% for the last waveform, and a Mackey-Glass time series prediction task with normalized root mean square error (NRMSE) of 0.2 for a 20-step prediction. Our work lays the foundations for low-power neuromorphic computing systems with magneto-electro-ferroelastic tunability, representing a further step towards developing future strain-mediated spintronic applications.
A pilot study of lymphodepletion intensity for peripheral blood mononuclear cell-derived neoantigen-specific CD8 + T cell therapy in patients with advanced solid tumors
Authors: Li Dandan , Chen Chao , Li Jingjing , Yue Jianhui , Ding Ya , Wang Hailun , Liang Zhaoduan , Zhang Le , Qiu Si , Liu Geng , Gao Yan , Huang Ying , Li Dongli , Zhang Rong , Liu Wei , Wen Xizhi , Li Bo , Zhang Xiaoshi , Zhang Xi , Xu Rui-Hua
Published: June 2023

Currently, the optimal lymphodepletion intensity for peripheral blood mononuclear cell-derived neoantigen-specific CD8 + T cell (Neo-T) therapy has yet to be determined. We report a single-arm, open-label and non-randomized phase 1 study (NCT02959905) of Neo-T therapy with lymphodepletion at various dose intensity in patients with locally advanced or metastatic solid tumors that are refractory to standard therapies. The primary end point is safety and the secondary end points are disease control rate (DCR), progression-free survival (PFS), overall survival (OS). Results show that the treatment is well tolerated with lymphopenia being the most common adverse event in the highest-intensity lymphodepletion groups. Neo-T infusion-related adverse events are only grade 1–2 in the no lymphodepletion group. The median PFS is 7.1 months (95% CI:3.7-9.8), the median OS is 16.8 months (95% CI: 11.9-31.7), and the DCR is 66.7% (6/9) among all groups. Three patients achieve partial response, two of them are in the no lymphodepletion group. In the group without lymphodepletion pretreatment, one patient refractory to prior anti-PD1 therapy shows partial response to Neo-T therapy. Neoantigen specific TCRs are examined in two patients and show delayed expansion after lymphodepletion treatment. In summary, Neo-T therapy without lymphodepletion could be a safe and promising regimen for advanced solid tumors.
All-optical nuclear quantum sensing using nitrogen-vacancy centers in diamond
Authors: Bürgler B. , Sjolander T. F. , Brinza O. , Tallaire A. , Achard J. , Maletinsky P.
Published: June 2023

Solid state spins have demonstrated significant potential in quantum sensing with applications including fundamental science, medical diagnostics and navigation. The quantum sensing schemes showing best performance under ambient conditions all utilize microwave or radio-frequency driving, which poses a significant limitation for miniaturization, energy efficiency, and non-invasiveness of quantum sensors. We overcome this limitation by demonstrating a purely optical approach to coherent quantum sensing. Our scheme involves the 15N nuclear spin of the Nitrogen-Vacancy (NV) center in diamond as a sensing resource, and exploits NV spin dynamics in oblique magnetic fields near the NV’s excited state level anti-crossing to optically pump the nuclear spin into a quantum superposition state. We demonstrate all-optical free-induction decay measurements—the key protocol for low-frequency quantum sensing—both on single spins and spin ensembles. Our results pave the way for highly compact quantum sensors to be employed for magnetometry or gyroscopy applications in challenging environments.
Modeling the potential health impact of prospective Strep A vaccines
Authors: Giannini Fiona , Cannon Jeffrey W. , Cadarette Daniel , Bloom David E. , Moore Hannah C. , Carapetis Jonathan , Abbas Kaja
Published: June 2023

The World Health Organization published the preferred product characteristics for a Group AStreptococcus(Strep A) vaccine in 2018. Based on these parameters for the age of vaccination, vaccine efficacy, duration of protection from vaccine-derived immunity, and vaccination coverage, we developed a static cohort model to estimate the projected health impact of Strep A vaccination at the global, regional, and national levels and by country-income category. We used the model to analyse six strategic scenarios. Based on Strep A vaccine introduction between 2022 and 2034 for the primary scenario, we estimated vaccination at birth for 30 vaccinated cohorts could avert 2.5 billion episodes of pharyngitis, 354 million episodes of impetigo, 1.4 million episodes of invasive disease, 24 million episodes of cellulitis, and 6 million cases of rheumatic heart disease globally. Vaccination impact in terms of burden averted per fully vaccinated individual is highest in North America for cellulitis and in Sub-Saharan Africa for rheumatic heart disease.
Study of the mining and aquifer interactions in complex geological conditions and its management
Authors: Huang Wanpeng , Sui Le , Wang Yanmin , Zhang Chengguo , Jiang Donghai , Cai Xianwei , Yang Zhixiang
Published: June 2023

The interaction of mining and the surface water or aquifer system in varying overburden strata conditions is one of the most critical aspects of sustainable mining practices, that can lead to water loss or water inrush into openings. This paper examined this phenomenon in a complex strata condition via a case study, and proposed a new mining design to minimize the impact of longwall mining on the overlaying aquifer. A range of factors have been identified contributing to the potential disturbance of the aquifer, including the extent of the water-rich area, the characteristics of overburden rock units, and the development height of the water-conducting fracture zone. In this study, the transient electromagnetic method and the high-density three-dimensional electrical method were used to identify two areas prone to water inrush danger in the working face. The vertical range of the water-rich abnormal area 1 is 45–60 m away from the roof, with an area of 3334 m2. The vertical range of the water-rich abnormal area 2 is 30–60 m away from the roof, with an area of approximately 2913 m2. The bedrock drilling method was used to determine that the thinnest part of the bedrock, with a thickness of approximately 60 m, and the thickest part, with a thickness of approximately 180 m. The maximum mining-induced height of the fracture zone was 42.64 m using empirical method, theoretical prediction based on the rock stratum group, field monitoring. In summary, the high risk area was determined, and the analysis shows that the size of the water prevention) pillar was 52.6 m, which was smaller than the safe water prevention pillar actually set in the mining range. The research conclusion provides important safety guidance significance for the mining of similar mines.
Association of hypertension and insulin resistance in individuals free of diabetes in the ELSA-Brasil cohort
Authors: Castro Luísa , Brant Luísa , Diniz Maria de Fátima , Lotufo Paulo , Bensenor Isabela Judith , Chor Dora , Griep Rosane , Barreto Sandhi Maria , Ribeiro Antonio Luiz
Published: June 2023

Insulin resistance (IR) is defined as the subnormal response to insulin action on its target tissues. Studies suggest that IR may increase the risk of hypertension, but the results are inconsistent and it is not known whether such an effect is independent of overweight/obesity. We aimed to evaluate the association between IR and the incidence of prehypertension and hypertension in the Brazilian population and whether this association is independent of overweight/obesity. In 4717 participants of the Brazilian Longitudinal Study of Adult’s Health (ELSA-Brasil), free of diabetes and cardiovascular disease at baseline (2008–2010), we investigated the incidence of prehypertension and hypertension after a mean follow-up of 3.8 ± 0.5 years. Insulin resistance at baseline was assessed by the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) index, defined if above the 75th percentile. The risk of IR-associated prehypertension/hypertension was estimated by multinomial logistic regression after adjustment for confounding factors. Secondary analysis were stratified by body mass index. The mean (SD) age of participants was 48 (8) years, 67% were women. The 75th percentile of HOMA-IR at baseline was 2.85. The presence of IR increased the chance of developing prehypertension by 51% (95% CI 1.28–1.79) and hypertension by 150% (95% CI 1.48–4.23). In individuals with BMI < 25 kg/m2, the presence of IR remained associated with the incidence of prehypertension (OR 1.41; 95% CI 1.01–1.98) and hypertension (OR 3.15; 95% CI 1.27–7.81). In conclusion, our results suggest that IR is a risk factor for hypertension, regardless of the presence of overweight or obesity.
Investigation of differences in susceptibility of Campylobacter jejuni strains to UV light-emitting diode (UV-LED) technology
Authors: Soro Arturo B. , Ekhlas Daniel , Marmion Maitiú , Scannell Amalia G. M. , Whyte Paul , Bolton Declan J. , Burgess Catherine M. , Tiwari Brijesh K.
Published: June 2023

Campylobacter jejuni remains a high priority in public health worldwide. Ultraviolet light emitting-diode technology (UV-LED) is currently being explored to reduce Campylobacter levels in foods. However, challenges such as differences in species and strain susceptibilities, effects of repeated UV-treatments on the bacterial genome and the potential to promote antimicrobial cross-protection or induce biofilm formation have arisen. We investigated the susceptibility of eight C. jejuni clinical and farm isolates to UV-LED exposure. UV light at 280 nm induced different inactivation kinetics among strains, of which three showed reductions greater than 1.62 log CFU/mL, while one strain was particularly resistant to UV light with a maximum reduction of 0.39 log CFU/mL. However, inactivation was reduced by 0.46–1.03 log CFU/mL in these three strains and increased to 1.20 log CFU/mL in the resistant isolate after two repeated-UV cycles. Genomic changes related to UV light exposure were analysed using WGS. C. jejuni strains with altered phenotypic responses following UV exposure were also found to have changes in biofilm formation and susceptibility to ethanol and surface cleaners.
Anatomy of nanomagnetic switching at a 3D topological insulator PN junction
Authors: Xie Yunkun , Vakili Hamed , Ganguly Samiran , Ghosh Avik W.
Published: June 2023

A P-N junction engineered within a Dirac cone system acts as a gate tunable angular filter based on Klein tunneling. For a 3D topological insulator with a substantial bandgap, such a filter can produce a charge-to-spin conversion due to the dual effects of spin-momentum locking and momentum filtering. We analyze how spins filtered at an in-plane topological insulator PN junction (TIPNJ) interact with a nanomagnet, and argue that the intrinsic charge-to-spin conversion does not translate to an external gain if the nanomagnet also acts as the source contact. Regardless of the nanomagnet’s position, the spin torque generated on the TIPNJ is limited by its surface current density, which in turn is limited by the bulk bandgap. Using quantum kinetic models, we calculated the spatially varying spin potential and quantified the localization of the current versus the applied bias. Additionally, with the magnetodynamic simulation of a soft magnet, we show that the PN junction can offer a critical gate tunability in the switching probability of the nanomagnet, with potential applications in probabilistic neuromorphic computing.