bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Power Flow Management for a Solar PV-Powered Soldier-Level Pico-Grid
Authors: Kunatsa Tawanda , Myburgh Herman C. , De Freitas Allan
Published: January 2024

Users ought to decide how to operate and manage power systems in order to achieve various goals. As a result, many strategies have been developed to aid in this regard. Optimal power flow management is one such strategy that assists users in properly operating and managing the supply and demand of power in an optimal way under specified constraints. However, in-depth research on optimal power flow management is yet to be explored when it comes to the supply and demand of power for the bulk of standalone renewable energy systems such as solar photovoltaics, especially when it comes to specific applications such as powering military soldier-level portable electronic devices. This paper presents an optimal power flow management modelling and optimisation approach for solar-powered soldier-level portable electronic devices. The OPTI toolbox in MATLAB is used to solve the formulated nonlinear optimal power flow management problem using SCIP as the solver. A globally optimal solution was arrived at in a case study in which the objective function was to minimise the difference between the power supplied to the portable electronic device electronics and the respective portable electronic device power demands. This ensured that the demand for solar-powered soldier-level portable electronic devices is met at all times in spite of the prohibitive case scenarios’ circumstances under the given constraints. This resolute approach underscores the importance placed on satisfying the demand needs of the specific devices while navigating and addressing the limitations posed by the existing conditions or constraints. Soldiers and the solar photovoltaic user fraternity at large will benefit from this work as they will be guided on how to optimally manage their power systems’ supply and demand scenarios. The model developed herein is applicable to any demand profile and any number of portable electronic device and is adaptable to any geographical location receiving any amount of solar radiation.
Influence of the Nano-Iron Oxide Adsorption-Enhanced Microstructured Charcoal Additives on the ANFO’s Properties
Authors: Biessikirski Andrzej , Gotovac Atlagić Suzana , Pytlik Mateusz , Kuterasiński Łukasz , Dworzak Michał , Twardosz Michał , Cała Marek , Jakóbczyk Joanna , Sukur Sunčica , Stopkowicz Agnieszka , Baziak Andrzej , Napruszewska Bogna Daria
Published: January 2024

The article presents the results of the third research stage on the potential microstructured charcoal additives in ANFO. The charcoal powder was liquid adsorption-treated with Fe in various ratios. Adding MC-Fe to ANFO changed the exothermic peak’s position from ca. 280 °C to 250 °C due to lower activation energy, which influenced the kinetics of the reaction. Bruceton’s test indicated that the MC-Fe addition to ANFO resulted in ca. 10% lower initiation energy in comparison with pure ANFO. However, the energy level did not influence the potential applicability of the additives. The fumes analysis indicated a lower concentration of CO for all tested samples; however, the concentration of NOx rose. The thermodynamic calculations confirmed the experimental results, which could be explained by the increased positive oxygen balance. Moreover, all analyses showed that the most promising blasting properties referred to the ANFO sample containing the microstructured charcoal additive of a C:Fe ratio of 4:1. Therefore, further research will be dedicated to advanced studies between the chemical composition of this specific ANFO sample and its physicochemical and blasting properties.
Response of Forest Bird Communities to Managed Landscapes in the Acadian Forest
Authors: Venier Lisa A. , Porter Kevin , Adams Gregory , McIlwrick Kenneth , Smenderovac Emily
Published: January 2024

The loss of mature forests is a known stressor of forest management on biodiversity. Mature forests provide unique habitat for forest birds. Here, we examine the capacity of mature forest stands embedded in an intensively managed landscape to provide habitat for landbird species that are associated with mature, unfragmented habitats. We carry this out by comparing bird communities in forest stands in three landscapes with a gradient of management activity. We examined community-level indicators (richness, diversity, abundance and community structure), and trait-level indicators (species groups associated with cavity nesting, mature forests, interior forests and area sensitivity). We found no obvious negative effects on bird communities, species and trait groups in forest stands in the most intensively managed landscape relative to the less intensively managed landscapes. Our ability to draw inferences about the influence of management intensity is limited due to lack of replication; however, these results do provide evidence that mature forest stands within intensively managed landscapes can provide valuable habitat to mature forest associates. There are often trade-offs between generating wood products from the forest and the provision of mature forest habitats. Research on forest birds can provide some of the necessary information for assessing the size and shape of those trade-offs and help to inform the conversation about the desired structure, function and composition of forests.
Effects of Different Altitudes on Castanopsis hystrix, the Top Community-Building Species in Southern Subtropical China: Rhizospheric Soil Chemical Properties and Soil Microbiota
Authors: Xue Guangyu , Xiong Junfei , Tang Li , Zhang Quanxin , Zeng Ji , Zhao Chenchi , Wu Junduo , Dong Shitao , Zhu Xueping
Published: January 2024

Castanopsis hystrix, a dominant canopy species in the subtropical forests of south China, is renowned for its high natural regeneration ability. Therefore, this paper took C. hystrix as the subject of study. Examining the variations in microbial diversity and community composition within the soil rhizosphere of C. hystrix across different elevation gradients, we explored how this community-forming species impacts soil microbial diversity and community structure and how soil microorganisms respond to changes in soil physicochemical properties due to altitude gradients. The results show: (1) soil samples from five altitudes established 1078 fungal OTU and 5595 bacterial OTUs. (2) Basidiomycota and ascomycota are dominant fungal groups in the soil, with Acidobacteria being the predominant bacteria for C. hystrix. (3) As altitude increases, fungal communities’ richness and diversity index peaks at 849 m; for bacterial communities, the richness index peaks at 387 m and the diversity index at 670 m. (4) Total phosphorus (TP), nitrate nitrogen (NO3−-N), hydrolyzed nitrogen (HN), total nitrogen (TN), and organic matter (SOM) are significant environmental factors affecting fungal community structure. At the same time, available potassium (AK) significantly influences the composition of bacterial communities. The study underscores the intricate relationship between altitude, soil properties, and microbial diversity, offering insights into how C. hystrix contributes to ecosystem resilience. Recommendations include enhancing phosphorus supplementation and controlling nitrogen deposition to maintain forest ecological integrity, additionally, the supply of potassium in the soil should also be a key consideration. Further research is necessary to understand the broader implications for biodiversity conservation and adaptive management strategies in the face of climate change.
Pyrogeography of the Western Great Plains: A 40-Year History of Fire in Semi-Arid Rangelands
Authors: McGranahan Devan Allen , Wonkka Carissa L.
Published: January 2024

This study describes spatial and temporal patterns in fire across the US Western Great Plains over the last 40 years. Although pyrogeographic studies have explored the nexus of fire patterns in relation to the bio-physical environment and socio-ecological trends, most of this research has focused on forested ecosystems and regions long known for conflict between wildfires and human development, especially at the wildland–urban interface. But evidence suggests large wildfire activity is increasing in the US Great Plains, and the Western Great Plains—a Land Resource Region comprised of four ecoregions, Northwestern Plains, High Plains, Nebraska Sandhills, and Southwestern Tablelands—not only contains some of the largest areas of rangeland in the US but also the highest concentration of public land in the Great Plains. As such, the Western Great Plains provides an opportunity to explore fire activity in primarily rural landscapes with a combination of public and private ownership, all dominated by rangeland vegetation. We combined several publicly-available datasets containing fire records between 1992 and 2020 to create two databases, one with georeferenced point data on 60,575 wildfire events in the region, and another with georeferenced perimeter data for 2665 fires. Ignition by humans was the dominant cause of fires. No ecoregion showed a statistically significant trend towards either increasing or decreasing the annual burned area. The Northwestern Plains had the most burned area and the greatest number of incidents—consistently around or above 1000 incidents per year since 1992—with the majority in July. The High Plains showed the greatest increase in annual fire incidence, never reaching more than 200–300 per year 1992–2009, and averaging above 1000 incidents per year since 2010. Few long-term trends in human population, weather, or fuel metrics appear strongly associated with fire patterns in any ecoregion, although the years 2006, 2012, and 2017 stood out for their levels of fire activity, and these years often frequently logged extreme values in wildland fuel metrics. These relationships merit much closer examination in the Western Great Plains, because like other rangeland-dominated landscapes, the fine fuels that comprise these wildland fuelbeds are much more responsive to fine-scale changes in moisture conditions. Rural Western Great Plains landscapes are a mosaic of public and private land ownership, and an increasing impact of wildfires on public grazing lands—which are often situated within other jurisdictions or ownership—will likely have an impact on rural livelihoods.
New Insights in Lifetime Migrations of Albacore Tuna (Thunnus alalunga, Bonnaterre, 1788) between the Southwest Indian and the Southeast Atlantic Oceans Using Otolith Microchemistry
Authors: Labonne Maylis , Darnaude Audrey M. , Fily Theotime , Petit Cécile , Nikolic Natacha , Parker Denham , Norman Stewart James , Clear Naomi , Farley Jessica , Eveson Jennifer Paige , Artetxe-Arrate Iraide , Murua Hilario , Davies Campbell , Marsac Francis
Published: January 2024

To clarify potential trans-oceanic connectivity and variation in the natal origin of albacore tuna (Thunnus alalunga) from the southwest Indian Ocean (SWI) and the southeast Atlantic (SA), lifetime otolith elemental signatures were assessed from 46 adults sampled from Reunion Island, and 26 juveniles(group 2+) sampled from two locations along the Atlantic coast of South Africa. LA-ICP-MS analysis was used to assess the multi-elemental composition in B, Ba, Mg, P, Sr, and Zn along the otolith edge (chemical signatures of the capture area), but also near the otolith primordium (spawning origin) and in an area located at 1400–1600 µm from it (nursery origin). Two groups of distinct near-primordium multi-elemental signatures, denoting potentially discrete spawning origins (SpO), were identified using hierarchical clustering. Each of the two SpO was found to contribute to the albacore stocks from all the areas sampled, suggesting a common spawning origin in some fish from the SWI and from the SA, and complex migrations between the two oceans. Three potentially discrete primary nursery sites were identified, each contributing to SA juvenile and SWI adult capture sites differently. The timing for the trans-oceanic movements observed for each albacore capture zone and its implications for local stock management are discussed.
Commercial Egg Replacers in Pound Cake Systems: A Comprehensive Analysis of Market Trends and Application
Authors: Halm Juliane , Sahin Aylin W. , Nyhan Laura , Zannini Emanuele , Arendt Elke K.
Published: January 2024

Replacing eggs without influencing pound cakes’ texture, appearance, and taste is challenging. Ovalbumin, the major protein in egg white, contributes to the structures of cakes by providing SH Groups that form a firm gel during baking. However, there is a shift in the consumers’ behaviour regarding health, well-being, animal welfare standards, and environmental concerns. To meet upcoming trends and consumer needs, 102 egg replacement products were launched globally to the best of the authors’ knowledge, with 20 of them advertised as suitable for baking applications. Ten locally available commercial egg replacers with a range of protein contents were chosen and applied in a pound cake model system to evaluate their functionality by evaluating cake and cake batter quality. Three different categories of egg replacements were chosen: replacers containing no protein (R1–R3), a low amount of protein (1–10 g/100 g; R4–R5), and a high amount of protein (>10 g/100 g; R6–R10). Those were compared to three control cakes containing powdered whole egg, fresh egg, and liquid whole egg. All the analysed egg replacers significantly differed from the control cakes, including low-protein egg replacement R4. Despite R4 achieving the highest specific volume (1.63 ± 0.07 mL/g) and comparable texture values, none of the examined egg replacers compared favourably with the egg control cakes regarding appearance, physical and textural properties, and nutritional value.
Printability of Nixtamalized Corn Dough during Screw-Based Three-Dimensional Food Printing
Authors: Rodríguez-Herrera Verónica Valeria , Umeda Takumi , Kozu Hiroyuki , Sasaki Tomoko , Kobayashi Isao
Published: January 2024

This study aimed to analyze the printability of corn-based dough during screw-based three-dimensional (3D) food printing (3DFP) by relating its rheological and mechanical properties to its screw-based 3DFP performance, with the objective of providing insights into the utilization of corn-based dough to produce 3D-printed foods. Screw-based 3DFP was performed using seven corn-based doughs with different nixtamalized corn flour (NCF) and water contents. Afterward, their rheological and mechanical properties were analyzed and associated with their screw-based 3DFP performance. The results showed that stable printability was obtained within a specific range of NCF content in the dough (30–32.5 wt%). Below this range, the 3D-printed foods flattened, while above it, the extrudability of the dough was affected. The printability of the dough was influenced by different rheological and mechanical properties, depending on the stage of the screw-based 3DFP process. During the extrusion stage, the loss tangent at nozzle strain, yield stress, apparent viscosity, and adhesiveness mainly affected the extrudability of the dough. In contrast, the loss tangent at minimum strain, elastic modulus, Young’s modulus, and hardness influenced the self-supporting stage. Therefore, it is important to find a balance between all of these properties, where stable extrudability and self-supporting of the 3D structure are achieved.
New Labeling Rules for Wine: Wine Alcohol-Derived Calories and Polyphenol Consumption on Health
Authors: Sánchez-Ortiz Antoni , Mateo-Sanz Josep , Mateos-Fernández Maria , Figueras Miriam
Published: January 2024

Alcohol content, proanthocyanins and anthocyanins influence wine quality. The composition of wine depends on the type of cultivar, location, environmental conditions, and management practices. Phenolic compounds have attracted considerable research interest due to their antioxidant properties and potential beneficial effects on human health. However, the low bioavailability of anthocyanins creates a major bottleneck in their ability to exert beneficial effects. Despite extensive research on the effects of wine on human health, no clear evidence has been obtained on the benefits of wine quality or geographic area of production on health conditions, such as metabolic syndrome. Five climatically and geologically distinct wines were evaluated. Based on recent studies, meta-analyses, and pooled analyses of wine composition, along with the predicted low bioavailability of polyphenol compounds, we estimated the efficacy of five geographically distinct wines according to gastrointestinal absorption and the effects of alcohol intake on both men and women, with a view to ascertaining whether geographical origin influences the antioxidant serum composition of wine. Data on the estimated consumption of wine suggest that the polyphenol contents are similar regardless of choice of wine/area, while different alcohol compositions affect the level of alcohol and calorie intake. Thus, moderate wine drinkers should be advised to control the habit, but without exceeding the dose considered a healthy threshold (up to 30–40 g of alcohol/day in men and 10–20 g of alcohol/day in women), given no medical contraindications are present. These results will add value to the framework of the last reform of the Common Agricultural Policy (CAP) adopted in December 2021, where the European Parliament and the Council introduced new labeling rules for the wine sector and aromatized wine products.
The Identification of a Strain for the Biological Purification of Soy Molasses to Produce Functional Soy Oligosaccharides and Optimize Purification Conditions
Authors: Yang Ran , Ma Jinghao , Wang Zechen , Du Yihua , Tian Shubin , Fan Guangsen , Liu Xiaoyan , Teng Chao
Published: January 2024

Soy molasses is rich in oligosaccharides like sucrose, stachyose, and raffinose, with stachyose and raffinose being functional oligosaccharides. Harnessing soy molasses for the production of functional soy oligosaccharides (FSO) can significantly elevate its value. Biological purification, a method leveraging the selective utilization of different carbon sources by microorganisms, allows for the specific removal of sucrose from soy molasses while preserving stachyose and raffinose, thereby increasing the FSO content. This research identified a yeast named YT312 with strong purification capabilities for soy molasses and optimized the purification conditions. The study revealed that yeast YT312 was Wickerhamomyces anomalus, exhibiting a broad range of growth temperatures and pH levels alongside a high tolerance to glucose, sucrose, and NaCl. Through single-factor and orthogonal experiments, it was established that under specific conditions—0.375% inoculum size, 30 °C fermentation temperature, 150 rpm shaking speed, 10-fold dilution ratio, pH of 7, and 12 h of fermentation—sucrose was completely removed from soy molasses, while functional raffinose and stachyose were retained at rates of 96.1% and 90.2%, respectively. Consequently, W. anomalus YT312 displayed exceptional characteristics for the biological purification of soy molasses and the production of FSO.
Regional Agriculture and Food Systems Amid the COVID-19 Pandemic: The Case of the Near East and North Africa Region
Authors: El Bilali Hamid , Ben Hassen Tarek
Published: January 2024

The COVID-19 pandemic affected agri-food systems worldwide. However, while the impacts differed from one country/region to another, the scholarly literature seems to focus on developed countries in the Global North. Consequently, this review scrutinizes the literature on the pandemic’s impacts in the Near East and North Africa (NENA) region. A search on the Web of Science in March 2023 generated 334 documents, of which 151 were eligible for inclusion in the systematic review. According to the bibliometric analysis, the most active nations are Saudi Arabia, Egypt, Jordan, the United Arab Emirates, and Lebanon. In general, the coverage of studies is better in the Gulf region than in the less developed countries of North Africa and those suffering from wars (cf. Libya, Syria, and Yemen). Studies generally focus on crop production and the downstream food chain (cf. distribution and consumption). While the pandemic influenced every pillar of food security, this research concentrates on access and utilization. Meanwhile, the lion’s share of the literature deals with the pandemic’s socio-economic effects, especially those linked to food (in)security and health. The pandemic, which laid bare the agri-food system vulnerabilities, should be seized to foster the transition towards more resilient agri-food systems in the NENA region.
Evaluation of Novel Preformed Particle Gel System for Conformance Control in Mature Oil Reservoirs
Authors: Almakimi Abdulaziz , Ben Ali Ahmed , Hussein Ibnelwaleed A. , Bai Baojun
Published: January 2024

To address challenges associated with excessive water production in mature oil reservoirs, this study introduces a carboxymethyl cellulose (CMC)-based material as a novel preformed particle gel (PPG) designed to plug excessive water pathways and redistribute the subsequent injected water toward unswept zones. Through microwave-assisted grafting copolymerization of CMC with acrylamide (AM), we successfully generated multi-sized dry particles within the range of 250–800 µm. Comprehensive analyses, including Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), have confirmed the chemical composition and morphology of the resulting carboxymethyl cellulose-grafted crosslinked polyacrylamide (CMC/PAMBA). Swelling kinetics and rheology tests were conducted to confirm the ability of this novel PPG system to perform at different reservoir conditions. The results of core flooding experiments showed that the CMC/PAMBA PPG is capable of plugging open fractures with a water breakthrough pressure gradient of up to 144 psi/ft. This preformed particle gel (PPG) system was designed specifically for application in Middle East reservoirs, which are distinguished by high salinity and elevated temperature levels. This PPG system is able to swell up to 10 times its original size in seawater and maintain a strength of about 1300 Pa at a temperature of 80 °C. Further optimization is conceivable to enhance injection efficiency and achieve superior plugging outcomes.
Influence of Socioeconomic Status on the Presence of Obstructive Coronary Artery Disease and Cardiovascular Outcomes in Patients Undergoing Invasive Coronary Angiography
Authors: Chung Jaehoon , Lim Woo-Hyun , Kim Hack-Lyoung , Joh Hyun Sung , Seo Jae-Bin , Kim Sang-Hyun , Zo Joo-Hee , Kim Myung-A
Published: January 2024

This retrospective study investigated the impact of socioeconomic status (SES) on patients at high risk of cardiovascular disease, focusing on obstructive coronary artery disease (CAD) presence and long-term cardiovascular outcomes in individuals undergoing invasive coronary angiography (ICA). Analyzing data from 9530 patients categorized by health insurance type (medical aid beneficiaries (MABs) as the low SES group; national health insurance beneficiaries (NHIBs) as the high SES group), this research explores the relationship between SES and outcomes. Despite a higher prevalence of cardiovascular risk factors, the MAB group exhibited similar rates of obstructive CAD compared to the NHIB group. However, over a median 3.5-year follow-up, the MAB group experienced a higher incidence of composite cardiovascular events, including cardiac death, acute myocardial infarction, coronary revascularization, and ischemic stroke, compared with the NHIB group (20.2% vs. 16.2%, p < 0.001). Multivariable Cox regression analysis, adjusting for potential confounders, revealed independently worse clinical outcomes for the MAB group (adjusted odds ratio 1.28; 95% confidence interval 1.07–1.54; p = 0.006). Despite comparable CAD rates, this study underscores the fact that individuals with low SES encounter an elevated risk of composite cardiovascular events, emphasizing the association between socioeconomic disadvantage and heightened susceptibility to cardiovascular disease, even among those already at high risk.
Manifestation of Pancytopenia Associated with COVID-19 as Paroxysmal Nocturnal Hemoglobinuria (PNH) and Aplastic Anemia (AA)
Authors: Aguilar Jeff Justin , Dhillon Vikram , Balasubramanian Suresh
Published: January 2024

We report two cases of pancytopenia in patients after recovering from a mild COVID-19, now presenting as paroxysmal nocturnal hemoglobinuria (PNH) and aplastic anemia. These cases illustrate a common pathway whereby a viral trigger causes the clonal expansion of a hematological disorder. Although the association of both cases with COVID-19 is temporal and COVID-19 may be an incidental diagnosis, the growing evidence related to the hematological effects of SARS-CoV-2 infection highlights the need for further investigation into the hematological consequences of COVID-19, particularly in the post-pandemic era.
Molecular Mechanisms in Pathophysiology of Mucopolysaccharidosis and Prospects for Innovative Therapy
Authors: Ago Yasuhiko , Rintz Estera , Musini Krishna , Ma Zhengyu , Tomatsu Shunji
Published: January 2024

Mucopolysaccharidoses (MPSs) are a group of inborn errors of the metabolism caused by a deficiency in the lysosomal enzymes required to break down molecules called glycosaminoglycans (GAGs). These GAGs accumulate over time in various tissues and disrupt multiple biological systems, including catabolism of other substances, autophagy, and mitochondrial function. These pathological changes ultimately increase oxidative stress and activate innate immunity and inflammation. We have described the pathophysiology of MPS and activated inflammation in this paper, starting with accumulating the primary storage materials, GAGs. At the initial stage of GAG accumulation, affected tissues/cells are reversibly affected but progress irreversibly to: (1) disruption of substrate degradation with pathogenic changes in lysosomal function, (2) cellular dysfunction, secondary/tertiary accumulation (toxins such as GM2 or GM3 ganglioside, etc.), and inflammatory process, and (3) progressive tissue/organ damage and cell death (e.g., skeletal dysplasia, CNS impairment, etc.). For current and future treatment, several potential treatments for MPS that can penetrate the blood–brain barrier and bone have been proposed and/or are in clinical trials, including targeting peptides and molecular Trojan horses such as monoclonal antibodies attached to enzymes via receptor-mediated transport. Gene therapy trials with AAV, ex vivo LV, and Sleeping Beauty transposon system for MPS are proposed and/or underway as innovative therapeutic options. In addition, possible immunomodulatory reagents that can suppress MPS symptoms have been summarized in this review.
Evaluation of the Therapeutic Effects of Harmine on Anaplastic Thyroid Cancer Cells
Authors: Baldini Enke , Cardarelli Silvia , Campese Antonio Francesco , Lori Eleonora , Fallahi Poupak , Virili Camilla , Forte Flavio , Pironi Daniele , Di Matteo Filippo Maria , Palumbo Piergaspare , Costanzo Maria Ludovica , D’Andrea Vito , Centanni Marco , Sorrenti Salvatore , Antonelli Alessandro , Ulisse Salvatore
Published: January 2024

Anaplastic thyroid carcinoma (ATC) is an extremely difficult disease to tackle, with an overall patient survival of only a few months. The currently used therapeutic drugs, such as kinase inhibitors or immune checkpoint inhibitors, can prolong patient survival but fail to eradicate the tumor. In addition, the onset of drug resistance and adverse side-effects over time drastically reduce the chances of treatment. We recently showed that Twist1, a transcription factor involved in the epithelial mesenchymal transition (EMT), was strongly upregulated in ATC, and we wondered whether it might represent a therapeutic target in ATC patients. To investigate this hypothesis, the effects of harmine, a β-carboline alkaloid shown to induce degradation of the Twist1 protein and to possess antitumoral activity in different cancer types, were evaluated on two ATC-derived cell lines, BHT-101 and CAL-62. The results obtained demonstrated that, in both cell lines, harmine reduced the level of Twist1 protein and reverted the EMT, as suggested by the augmentation of E-cadherin and decrease in fibronectin expression. The drug also inhibited cell proliferation and migration in a dose-dependent manner and significantly reduced the anchorage-independent growth of both ATC cell lines. Harmine was also capable of inducing apoptosis in BHT-101 cells, but not in CAL-62 ones. Finally, the activation of PI3K/Akt signaling, but not that of the MAPK, was drastically reduced in treated cells. Overall, these in vitro data suggest that harmine could represent a new therapeutic option for ATC treatment.
The 75–99 C-Terminal Peptide of URG7 Protein Promotes α-Synuclein Disaggregation
Authors: Dandurand Jany , Monné Magnus , Samouillan Valérie , Rosa Martina , Laurita Alessandro , Pistone Alessandro , Bisaccia Donatella , Matera Ilenia , Bisaccia Faustino , Ostuni Angela
Published: January 2024

Up Regulation Gene seven (URG7) is the pseudogene 2 of the transporter ABCC6. The translated URG7 protein is localized with its single transmembrane α-helix in the endoplasmic reticulum (ER) membrane, orienting the N- and C-terminal regions in the lumen and cytoplasm, respectively, and it plays a crucial role in the folding of ER proteins. Previously, the C-terminal region of URG7 (PU, residues 75–99) has been shown to modify the aggregation state of α-synuclein in the lysate of HepG2 cells. PU analogs were synthesized, and their anti-aggregation potential was tested in vitro on α-synuclein obtained using recombinant DNA technology. Circular dichroism (CD), differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, and microscopic techniques were used to assess the sample’s behavior. The results show that the peptides studied by themselves are prone to clathrate-like structure formation of variable stability. Aggregation of α-synuclein is accompanied by desolvation of its peptide chain and an increase in intermolecular β-sheets. The PU analogs all interact with α-synuclein aggregates and those possessing the most stable clathrate-like structures have the highest disaggregating effect. These findings suggest that the C-terminal region of URG7 may have a role in interacting and modulating α-synuclein structures and could be used to generate interesting therapeutic candidates as disaggregators of α-synuclein.
Transgenerational Transmission of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) Effects in Human Granulosa Cells: The Role of MicroRNAs
Authors: Gaspari Laura , Haouzi Delphine , Gennetier Aurélie , Granes Gaby , Soler Alexandra , Sultan Charles , Paris Françoise , Hamamah Samir
Published: January 2024

Endocrine-disrupting chemicals (EDCs) might contribute to the increase in female-specific cancers in Western countries. 2,3,7,8-tetrachlordibenzo-p-dioxin (TCDD) is considered the “prototypical toxicant” to study EDCs’ effects on reproductive health. Epigenetic regulation by small noncoding RNAs (sncRNAs), such as microRNAs (miRNA), is crucial for controlling cancer development. The aim of this study was to analyze transcriptional activity and sncRNA expression changes in the KGN cell line after acute (3 h) and chronic (72 h) exposure to 10 nM TCDD in order to determine whether sncRNAs’ deregulation may contribute to transmitting TCDD effects to the subsequent cell generations (day 9 and day 14 after chronic exposure). Using Affymetrix GeneChip miRNA 4.0 arrays, 109 sncRNAs were found to be differentially expressed (fold change < −2 or >2; p-value < 0.05) between cells exposed or not (control) to TCDD for 3 h and 72 h and on day 9 and day 14 after chronic exposure. Ingenuity Pathway Analysis predicted that following the acute and chronic exposure of KGN cells, sncRNAs linked to cellular development, growth and proliferation were downregulated, and those linked to cancer promotion were upregulated on day 9 and day 14. These results indicated that TCDD-induced sncRNA dysregulation may have transgenerational cancer-promoting effects.
A Sub-Group of Kidney-Transplant Recipients with Highly Aggressive Squamous Cell Carcinoma Expressing Phosphorylated Serine392p53
Authors: Hamdan Diaddin , Gardair Charlotte , Pamoukdjian Frédéric , Peraldi Gardin Marie-Noëlle , Nakouri Inès , Leboeuf Christophe , Janin Anne , Lebbé Céleste , Battistella Maxime , Bousquet Guilhem
Published: January 2024

Cutaneous squamous cell carcinomas in kidney-transplant recipients are frequent, with an increasing incidence linked to long immunosuppression durations and exposure to ultraviolet radiation. p53 is at the cornerstone of ultraviolet-induced DNA damage, but the role of p53 post-translational modifications in this context is not yet deciphered. Here, we investigated the phosphorylation status of p53 at Serine 392 in 25 cutaneous squamous cell carcinomas in kidney-transplant recipients, compared with 22 non-transplanted patients. Cutaneous squamous cell carcinomas in transplanted patients occurred after a median period of 19 years of immunosuppression, with a median number of 15 cutaneous squamous cell carcinomas and more aggressive histological and clinical characteristics. There was no significant difference between Ki67, p53, and pSer392p53 expression in the two groups. Using principal component analysis, we identified a cluster of exclusively transplanted patients with a median of 23 years of immunosuppression duration, significantly more aggressive biological characteristics, and higher pSer392p53 expression. pSer392p53 was expressed in the whole tumor, suggesting an early carcinogenic event in the course of prolonged immunosuppression. This high, diffuse pSer392p53 expression, corresponding to a high level of DNA damage, might be useful to identify aggressive cutaneous squamous cell carcinomas in kidney-transplant recipients to treat them more aggressively.
The Neurovascular Unit as a Locus of Injury in Low-Level Blast-Induced Neurotrauma
Authors: Elder Gregory A. , Gama Sosa Miguel A. , De Gasperi Rita , Perez Garcia Georgina , Perez Gissel M. , Abutarboush Rania , Kawoos Usmah , Zhu Carolyn W. , Janssen William G. M. , Stone James R. , Hof Patrick R. , Cook David G. , Ahlers Stephen T.
Published: January 2024

Blast-induced neurotrauma has received much attention over the past decade. Vascular injury occurs early following blast exposure. Indeed, in animal models that approximate human mild traumatic brain injury or subclinical blast exposure, vascular pathology can occur in the presence of a normal neuropil, suggesting that the vasculature is particularly vulnerable. Brain endothelial cells and their supporting glial and neuronal elements constitute a neurovascular unit (NVU). Blast injury disrupts gliovascular and neurovascular connections in addition to damaging endothelial cells, basal laminae, smooth muscle cells, and pericytes as well as causing extracellular matrix reorganization. Perivascular pathology becomes associated with phospho-tau accumulation and chronic perivascular inflammation. Disruption of the NVU should impact activity-dependent regulation of cerebral blood flow, blood–brain barrier permeability, and glymphatic flow. Here, we review work in an animal model of low-level blast injury that we have been studying for over a decade. We review work supporting the NVU as a locus of low-level blast injury. We integrate our findings with those from other laboratories studying similar models that collectively suggest that damage to astrocytes and other perivascular cells as well as chronic immune activation play a role in the persistent neurobehavioral changes that follow blast injury.
“Like a Virtual Family Reunion”: Older Adults Defining Requirements for an Augmented Reality Communication System
Authors: Mikhailova Veronika , Conde Melisa , Döring Nicola
Published: January 2024

Leading a socially engaged life is beneficial for the well-being of older adults. Immersive technologies, such as augmented reality (AR), have the potential to provide more engaging and vivid communication experiences compared to conventional digital tools. This qualitative study adopts a human-centered approach to discern the general attitudes and specific requirements of older adults regarding interpersonal communication facilitated by AR. We conducted semi-structured individual interviews with a sample of N = 30 older adults from Germany. During the interviews, participants evaluated storyboard illustrations depicting a fictional AR-enabled communication scenario centered around a grandparent and their adult grandchildren, which were represented as avatars within the AR environment. The study identified technological, emotional, social, and administrative requirements of older adults regarding the AR communication system. Based on these findings, we provide practical recommendations aimed at more inclusive technology design, emphasizing the significance of addressing the emotional needs of older adults, especially the perceived intimacy of AR-based interpersonal communication. Acknowledging and catering to these emotional needs is crucial, as it impacts the adoption of immersive technologies and the realization of their social benefits. This study contributes to the development of user-friendly AR systems that effectively promote and foster social engagement among older adults.
KEGGSum: Summarizing Genomic Pathways
Authors: David Chaim , Kondylakis Haridimos
Published: January 2024

Over time, the renowned Kyoto Encyclopedia of Genes and Genomes (KEGG) has grown to become one of the most comprehensive online databases for biological procedures. The majority of the data are stored in the form of pathways, which are graphs that depict the relationships between the diverse items participating in biological procedures, such as genes and chemical compounds. However, the size, complexity, and diversity of these graphs make them difficult to explore and understand, as well as making it difficult to extract a clear conclusion regarding their most important components. In this regard, we present KEGGSum, a system enabling the efficient and effective summarization of KEGG pathways. KEGGSum receives a KEGG identifier (Kid) as an input, connects to the KEGG database, downloads a specialized form of the pathway, and determines the most important nodes in the graph. To identify the most important nodes in the KEGG graphs, we explore multiple centrality measures that have been proposed for generic graphs, showing their applicability to KEGG graphs as well. Then, we link the selected nodes in order to produce a summary graph out of the initial KEGG graph. Finally, our system visualizes the generated summary, enabling an understanding of the most important parts of the initial graph. We experimentally evaluate our system, and we show its advantages and benefits.
Reproductive Behavior and Development of the Global Insect Pest, Cotton Seed Bug Oxycarenus hyalinipennis
Authors: Saveer Ahmed M. , Hu Jing , Strickland Jaime , Krueger Robert , Clafford Shannon , Zhang Aijun
Published: January 2024

Understanding the fundamental life cycle and reproductive behavior of a pest insect is essential for developing efficient control strategies; however, much of this knowledge remains elusive for a multitude of insects, including the cotton seed bug, Oxycarenus hyalinipennis. Here, we report the results of our comprehensive study on the cotton seed bug’s life cycle, including mating behavior, adult lifespan, and egg-to-adulthood development. Our findings showed that adult males and females began mating as early as three days after emerging (75%), and the frequency of mating increased to 100% by the fifth day. Mated females commenced oviposition on cotton seeds as early as two days after mating, with a cumulative mean number of 151 fertile eggs oviposited during the first oviposition cycle. Furthermore, around 10% of eggs from both mated and unmated females remained unfertilized. The first instar nymphs began emerging approximately seven days following oviposition. To track their development, we monitored the newly hatched nymphs daily until they reached adulthood. There were five nymphal stages, which cumulatively took roughly 28 to 30 days. Notably, mating positively influenced the survivorship and lifespan of adult O. hyalinipennis. Mated males and females exhibited median lifespans of 28 and 25 days, respectively. In contrast, unmated males and females only lived for a median lifespan of 9.5 days, about one-third that of the mated O. hyalinipennis. Our study provides key insights into the O. hyalinipennis life history for new IPM strategies.
Unveiling the Web: Exploring the Multifaceted Role of Neutrophil Extracellular Traps in Ocular Health and Disease
Authors: Adeeb Salma , Arabi Tarek Ziad , Shah Hassan , Alsalameh Sulaiman , Abu-Shaar Mylia , El-Sibai Abduljalil Mohamed , Alkattan Khaled , Yaqinuddin Ahmed
Published: January 2024

Neutrophil extracellular traps (NETs) play an essential role in antimicrobial defense. However, NETs have also been shown to promote and mediate a wide spectrum of diseases, including cancer, diabetes mellitus, cardiovascular diseases, and ocular diseases. Data regarding NETs in ocular diseases remain limited. In physiological conditions, NETs protect the eye from debris and cleave proinflammatory cytokines, including several interleukins. On the other hand, NETs play a role in corneal diseases, such as dry eye disease and ocular graft-versus-host disease, where they promote acinar atrophy and delayed wound healing. Additionally, NET levels positively correlate with increased severity of uveitis. NETs have also been described in the context of diabetic retinopathy. Although increased NET biomarkers are associated with an increased risk of the disease, NETs also assist in the elimination of pathological blood vessels and the regeneration of normal vessels. Targeting NET pathways for the treatment of ocular diseases has shown promising outcomes; however, more studies are still needed in this regard. In this article, we summarize the literature on the protective roles of NETs in the eye. Then, we describe their pathogenetic effects in ocular diseases, including those of the cornea, uvea, and retinal blood vessels. Finally, we describe the therapeutic implications of targeting NETs in such conditions.
Correlation between Ultrasound Peak Systolic Velocity and Angiography for Grading Internal Carotid Artery Stenosis
Authors: Tătaru Dan-Alexandru , Olinic Maria , Homorodean Călin , Ober Mihai-Claudiu , Spînu Mihail , Lazăr Florin-Leontin , Onea Laurențiu , Olinic Dan-Mircea
Published: January 2024

(1) Background: The success of carotid revascularization depends on the accurate grading of carotid stenoses. Therefore, it is important for every vascular center to establish its protocols for the same. In this study, we aimed to determine the peak systolic velocity (PSV) thresholds that can predict moderate and severe internal carotid artery (ICA) stenoses. (2) Methods: To achieve this, we enrolled patients who underwent both duplex ultrasound (DUS) and invasive carotid artery digital subtraction angiography (DSA). The degree of ICA stenosis was assessed using the North American Symptomatic Carotid Endarterectomy Trial (NASCET) and the European Carotid Surgery Trial (ECST) protocols. The PSV thresholds were determined using receiver operating characteristic (ROC) curves. (3) Results: Our study included 47 stenoses, and we found that the PSV cut-off for predicting ≥70% NASCET ICA stenoses was 200 cm/s (sensitivity 90.32%, specificity 93.75%). However, PSV did not correlate significantly with ≥50% NASCET ICA stenoses. On the other hand, the optimal PSV threshold for predicting ≥80% ECST ICA stenoses was 180 cm/s (sensitivity 100%, specificity 81.82%). (4) Conclusions: Based on our findings, we concluded that PSV is a good and simple marker for the identification of severe stenoses. We found that PSV values correlate significantly with severe NASCET and ECST stenoses, with 200 cm/s and 180 cm/s PSV thresholds. However, PSV was not reliable with moderate NASCET stenoses. In such cases, complementary imaging should be used.
Mild Cognitive Impairment Progression and Alzheimer’s Disease Risk: A Comprehensive Analysis of 3553 Cases over 203 Months
Authors: Öksüz Nevra , Ghouri Reza , Taşdelen Bahar , Uludüz Derya , Özge Aynur
Published: January 2024

This study aimed to elucidate the long-term progression of mild cognitive impairment (MCI) within a comprehensive longitudinal dataset, distinguish it from healthy aging, explore the influence of a dementia subtype on this progression, and identify potential contributing factors. Patients with prodromal and preclinical cases underwent regular neuropsychological assessments utilizing various tools. The study included a total of 140 participants with MCI, categorized into Alzheimer’s disease (AD) and non-AD subtypes. Our dataset revealed an overall progression rate of 92.8% from MCI to the clinical stage of dementia during the follow-up period, with an annual rate of 15.7%. Notably, all prodromal cases of Lewy body dementia/Parkinson’s disease (LBD/PDD) and frontotemporal dementia (FTD) advanced to clinical stages, whereas 7% of vascular dementia (VaD) cases and 8.4% of AD cases remained in the prodromal stage throughout follow-up. Furthermore, we observed a faster progression rate in MCI-AD cases compared to non-AD sufferers (53.9% vs. 35.5%, Entropy: 0.850). This study revealed significant cognitive changes in individuals with MCI over time. The mini-mental state examination (MMSE), global deterioration scale (GDS), and calculation tests were the most effective tests for evaluation of MCI. These findings may offer valuable insights for the development of personalized interventions and management strategies for individuals with MCI.
Transcatheter Aortic Valve Implantation (TAVI) Planning with Dual-Layer Spectral CT Using Virtual Monoenergetic Image (VMI) Reconstructions and 20 mL of Contrast Media
Authors: Fontana Federico , Piacentino Filippo , Gnesutta Aroa , Macchi Edoardo , Coppola Andrea , Saccomanno Angiola , Gatta Tonia , Recaldini Chiara , Minenna Manuela , Tamborini Claudio , Dossi Filippo , Ascenti Velio , Barbera Simone , Cicero Giuseppe , Carcano Giulio , Ascenti Giorgio , Castiglioni Battistina , Venturini Massimo
Published: January 2024

Transcatheter aortic valve implantation (TAVI) is a less invasive alternative to surgical implantation and its implementation is progressively increasing worldwide. We routinely perform pre-procedural aortic angiography CT to assess aortic dimensions and vascular anatomy. This study aims to evaluate the image quality of CTA for TAVI planning using dual-layer spectral CT, with virtual monoenergetic image reconstructions at 40 keV. Thirty-one patients underwent a CTA protocol with the injection of 20 mL of contrast media. Image quality was assessed by measuring the mean density in Hounsfield Units (HU), the signal-to-noise ratio, and the contrast-to-noise ratio in VMI reconstructions. Additionally, a blinded subjective analysis was conducted by two observers. The results showed significant enhancement at all sampled vascular levels with a gradual decrease in HU from proximal to distal regions. Favourable subjective ratings were given for all parameters, with greater variability in the evaluation of iliac axes. A significant negative correlation (p < 0.05) was observed between BMI and CA at all vascular levels, indicating reduced contrast enhancement with increasing BMI. Spectral CT, along with reducing iodine load, allows for obtaining high-quality images without a significant increase in noise. The reduction in iodine load can have positive implications in clinical practice, improving patient safety and resource efficiency.