bluuebunny/crossref_metadata_2025 huggingface dataset:
Patient-Derived Anti-NMDAR Antibody Disinhibits Cortical Neuronal Networks through Dysfunction of Inhibitory Neuron Output
Authors: Andrzejak Ewa , Rabinovitch Eshed , Kreye Jakob , Prüss Harald , Rosenmund Christian , Ziv Noam E. , Garner Craig C. , Ackermann Frauke
Published: March 2022

Anti-NMDA receptor (NMDAR) encephalitis is a severe neuropsychiatric disorder associated with autoantibodies against NMDARs, which cause a variety of symptoms from prominent psychiatric and cognitive manifestations to seizures and autonomic instability. Previous studies mainly focused on hippocampal effects of these autoantibodies, helping to explain mechanistic causes for cognitive impairment. However, antibodies' effects on higher cortical network function, where they could contribute to psychosis and/or seizures, have not been explored in detail until now. Here, we employed a patient-derived monoclonal antibody targeting the NR1 subunit of NMDAR and tested its effects onin vitrocultures of rodent cortical neurons, using imaging and electrophysiological techniques. We report that this hNR1 antibody drives cortical networks to a hyperexcitable state and disrupts mechanisms stabilizing network activity such as Npas4 signaling. Network hyperactivity is in part a result of a reduced synaptic output of inhibitory neurons, as indicated by a decreased inhibitory drive and levels of presynaptic inhibitory proteins, specifically in inhibitory-to-excitatory neuron synapses. Importantly, on a single-cell level hNR1 antibody selectively impairs NMDAR-mediated currents and synaptic transmission of cortical inhibitory neurons, yet has no effect on excitatory neurons, which contrasts with its effects on hippocampal neurons. Together, these findings provide a novel, cortex-specific mechanism of antibody-induced neuronal hyperexcitability, highlighting regional specificity underlying the pathology of autoimmune encephalitis.

SIGNIFICANCE STATEMENTIt is increasingly appreciated that the inadvertent activation of the immune system within CNS can underlie pathogenesis of neuropsychiatric disorders. Although the exact mechanisms remain elusive, autoantibodies derived from patients with autoimmune encephalitis pose a unique tool to study pathogenesis of neuropsychiatric states. Our analysis reveals that autoantibody against the NMDA receptor (NMDAR) has a distinct mechanism of action in the cortex, where it impairs function of inhibitory neurons leading to increased cortical network excitability, in contrast to previously described hippocampal synaptic mechanisms of information encoding, highlighting brain regional specificity. Notably, similar mechanism of NMDAR-mediated inhibitory hypofunction leading to cortical disinhibition has been suggested to underlie pathology of schizophrenia, hence our data provide new evidence for common mechanisms underlying neuropsychiatric disorders.
CLC Anion/Proton Exchangers Regulate Secretory Vesicle Filling and Granule Exocytosis in Chromaffin Cells
Authors: Comini Maddalena , Sierra-Marquez Juan , Guzman Gustavo , Franzen Arne , Willuweit Antje , Katona Istvan , Hidalgo Patricia , Fahlke Christoph , Guzman Raul E.
Published: March 2022

ClC-3, ClC-4, and ClC-5 are electrogenic chloride/proton exchangers that can be found in endosomal compartments of mammalian cells. Although the association with genetic diseases and the severe phenotype of knock-out animals illustrate their physiological importance, the cellular functions of these proteins have remained insufficiently understood. We here study the role of twoClcn3splice variants, ClC-3b and ClC-3c, in granular exocytosis and catecholamine accumulation of adrenal chromaffin cells using a combination of high-resolution capacitance measurements, amperometry, protein expression/gene knock out/down, rescue experiments, and confocal microscopy. We demonstrate that ClC-3c resides in immature as well as in mature secretory granules, where it regulates catecholamine accumulation and contributes to the establishment of the readily releasable pool of secretory vesicles. The lysosomal splice variant ClC-3b contributes to vesicle priming only with low efficiency and leaves the vesicular catecholamine content unaltered. The related Cl−/H+antiporter ClC-5 undergoes age-dependent downregulation in wild-type conditions. Its upregulation inClcn3−/−cells partially rescues the exocytotic mutant defect. Our study demonstrates how different CLC transporters with similar transport functions, but distinct localizations can contribute to vesicle functions in the regulated secretory pathway of granule secretion in chromaffin cells.

SIGNIFICANCE STATEMENTCl−/H+exchangers are expressed along the endosomal/lysosomal system of mammalian cells; however, their exact subcellular functions have remained insufficiently understood. We used chromaffin cells, a system extensively used to understand presynaptic mechanisms of synaptic transmission, to define the role of CLC exchangers in neurosecretion. Disruption of ClC-3 impairs catecholamine accumulation and secretory vesicle priming. There are multiple ClC-3 splice variants, and only expression of one, ClC-3c, in double Cl−/H+exchanger-deficient cells fully rescues the WT phenotype. Another splice variant, ClC-3b, is present in lysosomes and is not necessary for catecholamine secretion. The distinct functions of ClC-3c and ClC-3b illustrate the impact of expressing multiple CLC transporters with similar transport functions and separate localizations in different endosomal compartments.
New silvicultural approaches for multipurpose management in beech forests
Authors: Pelleri Francesco , Becagli Claudia , Sansone Dalila , Bianchetto Elisa , Bidini Claudio , Manetti Maria Chiara
Published: March 2022

Traditional thinning and stand silviculture had positive effects on tree stability, stand efficiency and tree growth if compared to the lack of management. Nevertheless, the tree oriented silviculture has proven to be a type of management best meets the multifunctionality pourpouses required by forests today. In Italy beech high forests are generally managed with traditional thinning from below ascribable to stand silviculture; in the last decades tree oriented silviculture, an innovative management system is spreading in Europe. The aim of the study is to compare two thinning types applied in two localities of central Italy, in beech stands deriving from shelterwood system, aged 45-50 years: stand silviculture (SS), tree-silviculure (TS) and control (C). All trees were periodically measured in order to assess growth, mortality and production from 2008 onwards. Crop trees DBH, selected for each silvicultural approach, was annually measured while, an evaluation of the stem quality, crown development and stability were carried out between the 1st and last thinnings. Differences in vertical and horizontal diversity stand structures were assessed. Results pointed out similar growth rate at stand level; while a superior growth in diameter, in dimension of the crown and stability of the crop trees were observed in TS. The TS has proven to be the management option that best meets the multifunctionality required by forests today.
Theatricality of women’s voice: An Embarrassing Position by Kate Chopin
Authors:   University of Bielsko-Biała, Poland, Kliś Agnieszka
Published: March 2022

The paper offers an analysis of An Embarrassing Position, the only extant and not very well-known theatre play by Kate Chopin. Even though it is only one-act-long, the play does not only contribute to the greatness of the artistry of the author, but it also subtly and wittily raises the question of societal imbalance and women’s right to self-deciding. Hence, the aim of this paper is both to expose the theatricality of women’s voice embedded in the play validating women’s stance and foretelling women’s emancipation, and to bring the play to the broader attention of academics as a unique, still underexamined, work of Chopin. In order to establish this light comedy play within a serious domain of women’s rights struggle, as well as to inscribe this short piece of work into the long list of Chopin’s writings which are pro feminist in their overtone, the paper uses hermeneutic analysis with elements of Derrida’s deconstructionist theory of différance, and employs Carl Jung’s idea of synchronicity. Derrida’s deconstruction serves to uncover the inner inconsistencies residing within the humorous lines of the play, whereas synchronicity provides the scaffold for the play’s events that bonds the analysis elements into one meaningful whole.
Severe Pediatric COVID-19 Pneumonia Treated With Adjuvant Anakinra
Authors: Stubbs Leigh A. , Szafron Vibha , Forbes Lisa R. , Musick Matthew A. , Gillispie Amanda E. , Sauer Hannah E. , Smith Valeria R. , Fasipe Titilope A. , Munoz Flor M. , Tejtel Kristen Sexson , Silva-Carmona Manuel , Vogel Tiphanie P. , Muscal Eyal
Published: March 2022

BACKGROUND AND OBJECTIVES

To compare previous hemophagocytic lymphohistiocytosis criteria with adult coronavirus disease 2019 (COVID-19)-associated hyperinflammatory syndrome (cHIS) criteria for the diagnosis of hyperinflammation in pediatric patients with COVID-19. The secondary objective was to assess treatment response to intravenous (IV) anakinra in these patients.

METHODS

This case series included children admitted to the PICU for COVID-19 pneumonia with hyperinflammation and treated with IV anakinra between July 2020 to April 2021. Hyperinflammatory criteria were determined for each patient. Clinical course, chest imaging, and inflammatory marker trends were assessed pre- and post-anakinra treatment.

RESULTS

All patients had a cHIS criteria score of ≥5. Two patients met 2004-hemophagocytic lymphohistiocytosis criteria. Only the patient that required extracorporeal membrane oxygenation met the H-Score cut-off value. All but one patient had a decrease in their inflammatory markers and improvement in clinical status with early initiation of adjunctive IV anakinra.

CONCLUSIONS

In this case series, adult cHIS criteria were successfully used to identify pediatric COVID-19 patients with hyperinflammation. Ferritin levels decreased after the early initiation of IV anakinra.
Hyperglycemia and Cytopenias as Signs of SARS-CoV-2 Delta Variant Infection in Preterm Infants
Authors: Boly Timothy J. , Reyes-Hernandez Melanie E. , Daniels Elizabeth C. , Kibbi Nadine , Bermick Jennifer R. , Elgin Timothy G.
Published: March 2022

Information regarding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections in premature infants remains limited. Early in the pandemic, several studies reported that the risk of infection in infants was relatively small and that affected infants had a milder disease than what was seen in adults. Since the increase of the delta variant (SARS-CoV-2 B.1.617.2) within the population, there have been increased reports of more severe disease in infants. We present 3 cases of premature, very low birth weight infants with confirmed SARS-CoV-2 infection who presented with significant hyperglycemia and bone marrow dysfunction. Two infants had presumed vertical transmission, and 1 infant was infected by respiratory transmission. Despite the mode of transmission, symptom onset and duration were similar in all infants. All resolved with symptomatic management. In the context of the continuing pandemic, evaluation for SARS-CoV-2 infection should be considered in premature very low birth weight infants who demonstrate certain patterns of acute metabolic and hematologic abnormalities.
Da Criação Artística à Representação do Imaginário e da Cultura Popular no Gênero História em Quadrinhos
Authors: Dos Santos Milena , Da Silva Cícero
Published: March 2022

Neste artigo, analisam-se elementos que caracterizam aspectos da criação artística, mímesis, representação do imaginário e da cultura popular no gênero História em Quadrinhos (HQs) produzido por acadêmicos do Curso de Licenciatura em Educação do Campo: Códigos e Linguagens – Artes e Música da Universidade Federal do Tocantins. Metodologicamente, realizou-se uma pesquisa participante, de abordagem qualitativa. Os resultados mostram que é preciso compreender a estética do gênero HQs, sua relação com a literatura, bem como a mímesis na produção artístico-literária. E um dos caminhos para entender as representações do imaginário e da cultura popular nas HQs perpassa pela criação estética e pela literatura.
Capitalismo de la seducción, exposición y emoción en la paulatina sociedad del ‘me gusta’
Authors: Briceño-Montilla Luis Alfonso
Published: March 2022

El capitalismo de la seducción, exposición y emoción, son una serie de analogías que involucran aspectos como la necesidad implícita de representación, similar a la dinámica consumista; nociones como autoexplotación, crisis de la representación, así como mecanismos y estrategias provenientes del campo de la psicología, son algunos de los elementos que se emparentan con actividades llevadas a cabo por las grandes tecnológicas (Facebook), como maniobras corporativistas de dominación en el universo de la red. Así, el objetivo del presente ensayo gira en torno a reflexionar crítica e interpretativamente sobre presupuestos como ideología de la necesidad, espectáculo, indignación, transparencia, atención, lenguaje emoji, “erotismo” como lucro, aceleración, circulación rauda de información, adicción y concentración de usuarios. La metodología implícita se situa el campo de la hermenéutica analógica, en aras de contribuir con un aporte teórico crítico de carácter social. En cuanto a las reflexiones finales, estas giran en torno a los memes como disrupción racional y lógica de circulación viral en consonancia con la alusión de sociedad del me gusta.
Ubiquitin-specific protease 8 inhibits lipopolysaccharide-triggered pyroptosis of human bronchial epithelial cells by regulating PI3K/AKT and NF-κB pathways
Authors: Liu Lu , Huan Liting , Zhang Yu , Wei Wei , Chen Zhihai , Xu Di , Huang Xiufeng , Tan Yaoxi , Li Hongxing
Published: March 2022

Asthma, characterized by dysfunction of airway epithelial cells, is regarded as a chronic inflammatory disorder in the airway. Ubiquitin-specific protease 8 (USP8) belongs to ubiquitin proteasome system and mediates the stability of E3 ligases. The anti-inflammatory effect of USP8 has been widely investigated in distinct diseases, while the role of USP8 in asthma remains elusive. Firstly, human bronchial epithelial cells (BEAS-2B) were treated with lipopolysaccharide, which reduced the cell viability of BEAS-2B and induced the secretion of lactate dehydrogenase (LDH). Moreover, the expression of USP8 was downregulated in BEAS-2B post lipopolysaccharide treatment. Secondly, overexpression of USP8 enhanced cell viability of lipopolysaccharide-treated BEAS-2B, and reduced the LDH secretion. USP8 overexpression also attenuated lipopolysaccharide-induced upregulation of TNF-α, IL-6, and IL-1β in BEAS-2B. Thirdly, lipopolysaccharide treatment promoted the expression of NLRP3 (NLR Family Pyrin Domain Containing 3), N-terminal domain of gasdermin D (GSDMD-N), caspase-1, IL-1β, and IL-18 in BEAS-2B, which was inhibited by USP8 overexpression. Lastly, USP8 overexpression decreased the phosphorylation of NF-κB, while it increased the phosphorylation of PI3K and AKT in lipopolysaccharide-treated BEAS-2B. In conclusion, USP8 inhibited lipopolysaccharide-triggered inflammation and pyroptosis in human bronchial epithelial cells by activating PI3K/AKT signaling and inhibiting NF-κB signaling pathway.
Towards the Conceptualization of Occupations and Professions’ Explosion
Authors:   Russian State Vocational Pedagogical University, Kislov Alexander G. , Shcherbina Elena Yu. ,   Russian State Vocational Pedagogical University
Published: March 2022

The paper proposes the mainstreaming of the conceptual potential of Yu. M. Lotman (1922–1993) and M. K. Petrov (1923–1987) scientific and philosophical heritage relating to the theory of explosive complexity, increasing diversity and instability, which our society has recently been observing in occupational, vocational and professional spheres and the related social institutions (professional associations, professional education, professional guidance, etc.). Heterogeneous and heteronomic being-in-common (Fr. partage) in light of the works of J.-L. Nancy (b. 1940) is the most important heuristic intuition of the proposed updating. It allows one to think through a single, but flexible, multi-directionally transformable and polysemantic framework for the future theory of rapidly updated professional diversity, its explanation, forecast of likely development scenarios and strategies for self-determination within it and management strategies in relation to it. The authors present the conceptual unfolding of the intuition of being-in-common (partage) to be compatible with both the theoretical provisions of the semiotics of culture of Yu. М. Lotman and the socio-cultural dynamics of M. K. Petrov. Professionology is still searching for a methodological platform that would complement its retrospective generalizations and interdisciplinary discontinuity. The actualization of the potential of the concept of compatibility allows it to consolidate multidisciplinary research and its results and to guide them along the path of careful and, at the same time, bold theoretical construction in accordance with what social processes declare themselves to be. The authors observe the readiness of today’s multidisciplinary discourse to unite on such a methodological platform that allows for not to being lost in it under the influence of priorities and presumptions that are inevitable for the methodology, but, at the same time, focusing the collective efforts of occupational researchers on the most promising and practice-oriented aspects of the subject of their research.
Two-face Chronos. Glossary of the Reversed Time
Authors:   Emory University, Epstein Mikhail N.
Published: March 2022

The article explores the history of the concept “retromania” and analyzes possibilities and specific features of its application to the Russian socio-cultural situation of the 21-st century. The distinction of Russia retromania resides, in the first place, in the scale of its spread incompatible with the scale of pop-culture in the space of which this term was born. Secondly, Russian retromania faces away from the recent past; it is directed to the deep past reaching archaic layers of culture. Retromania acquires forms of a new political metaphysics — necrocracy, total involvement of heroes of the past, especially war participants as witnesses and actors of modernity. The paper offers symbolic forms of work with the past and practices derived from them. The general strategy of necrocacy is depicted through tantalizations — the prevalence of death value regarding eros at all levels of social psychology and ideology. Chronos is also considered as time that changes its direction and flies backwards. The article introduces new forms of the existence of Russian Chronos marked with the concepts of totalgy, chronocide, the newest Middle Ages, uchronia, schizofascism, New Moscovia, topochron, and chronocracy. Totalgy is of an emotional nature and is expressed in a longing for totality, totalitarialism, and is fixed in ideology. Chronocide is violence towards the natural course of time. And in this respect, one can observe continuity with Soviet ideology where all the rhetoric used to be project-based as well as today the past dominates the present. Uchronia implies the construction of a state of timelessness, and the newest Middle Ages are characterized by the increase in religious and political fundamentalism. There is a direct way from fundamentalism to fascism which manifests itself as schizofascism, i.e. fascism under the guise of combating fascism. Such concepts as New Moscovia expressed in the dominance of Moscow in mass consciousness and topochron — the prevalence of topos over chromos were used to analyze the topos of current “retromanian” Russia. The article offers an optimistic scenario of the development expressed by the concept of chonocracy when time in its metaphysical sacred meaning defeats the profane politics of retromania.
On the new Meaning of the Institution of the Presidency
Authors:   National Research University Higher School of Economics, Krasnov Mikhail A.
Published: March 2022

The article raises the problem of the authoritarian potential rooted in the current institution of the presidency. The author’s position is that an institution of the presidency evolved from the institution of the monarch, and along with it, inherited its public law meaning. At the same time, the monarchical properties could not be "delegated" to the president only due to the fundamentally different nature of this institution. That is why President is not a republican copy of the monarch, but a replica. When the president assumes the highest position in the state system, as head of state, he tends to acquire monarchic qualities. Not because he wants to become a monarch, but because he wants to make his power virtually unlimited and indefinite, i.e. to establish a regime of personal power. This is an ontological danger, although certain conditions are necessary to realize an authoritarian rebirth. This paper outlines a new model for the institution of the presidency. In the author’s opinion, it can significantly reduce the likelihood of an authoritarian trend. The essence of the proposed model is a fundamental change in the very meaning of the head of state. It involves the transition from his role of a leader (whether actual or formal/nominal) to that of the guardian of the constitutional order. Here, the comprehension of the constitutional order itself is broad. In other words, it is not only an established system of state bodies acting within their competence but also the compliance of their activities with constitutional values (constitutionalism). The author argues that the new meaning of the institution of the presidency is not a reduction in the amount of power. In the author’s view, it is, first, a change in the focus and nature of power and, second, ensuring the political neutrality of the head of state. A set of measures can ensure the latter. And among them, the main is the complete removal of the President from the processes of current state administration. It does not mean for President to have only a ceremonial role. It means to focus exclusively on the protection and defense of the constitutional order. The paper concludes by describing some specific parameters that characterize the new understanding of the president.
Constructing “a New Person”: Breaking the Links Between the Natural and the Sociocultural
Authors:   Ural Federal University, Merenkov Anatoly V.
Published: March 2022

The revision of the centuries-old culture of relationships between people in the implementation of sexual orientations and attitudes, the needs for ensuring racial equality, which is taking place in several countries around the world, requires proper sociological analysis, as it affects hundreds of millions of people. Some propose to change the established ideas about the influence of nature on human behaviour when communicating with representatives of the other sex, race. In the mass media, in the statements of politicians, the idea of refusing to name people based on their belonging to the male or female sex began to be approved. It should be determined not on the basis of visible natural features but constructed by a new gender culture. In 2020C, changes in interpreting the role of the natural and the socio-cultural in racial relations resulted in the emergence of new methods of fighting for equality between whites and blacks. The ideology of expanding the advantages of African Americans in all spheres of public life, “repentance” of whites for the fact that among their ancestors were slave owners, is spreading. The article shows that what unites these two processes is an attempt to redefine the role of the nature-given external differences of people in shaping a culture of interaction between the two sexes, of different races, both in the past and at present. From an analysis of the history of relations between men and women, people depending on their skin colour, it is concluded that individuals adopt and implement norms, rules of behaviour that do not lead to the violent suppression of what is given to them by nature. The policy of constructing a new person, guided by the norms of culture, forcing the complete rejection of what is set by nature, as well as was created by previous generations, leads to the destruction of the entire historically established system of organizing people’s social life.
Building an Information and Communication Culture in the Modern Digital Environment
Authors:   Tyumen State Institute of Culture, Skulmovskaya Lubov G. , Antonova Lyudmila Y. ,   Tyumen State Institute of Culture, Kudinova Olga S. ,   Tyumen State University
Published: March 2022

The study explores the problems of forming an information and communication culture in a modern digital environment. In the context of the concept of the information society, the paper considers the characteristics of various types of culture functioning in the contemporary digital environment: information, electronic, screen, Internet culture. It also reveals their typical features and distinctive features. Information culture is a type of culture that includes a system of views, ethical attitudes, rules and norms, based on information as value, and material media — means of transmission (press, radio, telephony, television and the Internet). As a synonym to “information culture,” the concept of “electronic culture” is often used. Its fundamental feature is virtual electronic platforms broadcasting cultural achievements. Screen culture is considered a kind of product of the information era, formed during the collision of a person with screen display media and inseparably associated with technological progress. Internet culture can become a particular type of culture, which includes the use of the Web as a platform for realizing the needs of communication and self-expression, developing creativity through the creation, use, transformation of virtual objects, often similar to real ones, but with a special, unique appearance. The work identified components of information culture, analyzed its interpretations within the framework of cultural, information, activity and personal approaches. In the study of electronic, screen and Internet culture, there are also several scientific approaches: cultural, philological, philosophical, psychological. The authors indicate that at this stage of the development of society, there is a formation of a new social type of person (“information”) and the nature of social relations. The authors considered the phenomenon of information and communication culture as a component of the information society and examined its formation in the digital environment using areas of education and culture as an example.
J. W. Goethe: The Way of Life and Philosophy of Art
Authors:   Ural Federal University, Pertsev Aleksandr V.
Published: March 2022

Is it possible today, within the framework of the history of philosophy, to reconstruct the life path of one of the philosophers, resorting to the recipes of subsequent researchers — especially when these followers did their creative works a couple of centuries after those who they should describe? Today empiricism dominates in the humanities: flat facts are grasped on a large scale and summed up in everyday spaces, and the results are correct only here and now. It is the way to write the biographies of the “big masses”. But some geniuses — those who live “in isolation” — not relying on standards, not laying flat, unwillingly turning their lives back, rising above the bustle. To what extent can one describe this path in the manner of existentialism — moving from inauthentic existence to borderline situations and, further, to genuine existence, which was never properly built?
Projeto Terapêutico Singular no manejo de casos complexos: relato de experiência no PET-Saúde Interprofissionalidade
Authors: Ferreira Maynara Guaripuna , Ferreira Kathleen Hanna Gonçalves , Souza Cibelle Regina Piani de , Ortiz Priscila Cherley de Araújo , Almeida Rodrigo Guimarães dos Santos , Silva Adélia Delfina da Motta
Published: March 2022

Abstract: Introduction: The Program Education through Work for Health (PEW-Health) was created in 2008 as a partnership between the Ministries of Health and Education, promoting the teaching-service-management-community integration, providing opportunities for professionals, students, teachers, and service users, in line with the needs of SUS, and having the topic of interprofessionality for its ninth edition (2019-2021). This report aims to share interprofessional learning based on the experience of a singular therapeutic project (STP) for a complex case within the PEW-Health activities of a university in the Midwest region, carried out in a Family Health Unit (FHU) in the municipality of Campo Grande, Mato Grosso do Sul (MS). Experience report: Students from a tutorial group of PEW-Health Interprofessionality participated in this experience, as well as preceptors, professionals from the Extended Nucleus of Family Health and Primary Care (ENFH-PC), and a tutor, who was a teacher in a Medical School. The STP was developed with an elderly patient with polypharmacy and the following chronic conditions: diabetes mellitus (DM), depression (DEP) and systemic arterial hypertension (SAH), monitored during the period from August 2019 to February 2020, prior to the COVID-19 pandemic, in a FHU in Campo Grande-MS, selected by the team due to the complexity of the case. Discussion: Through the STP, the group had the opportunity to evaluate, assist and perform practices to strengthen the patient’s “happiness project”. The STP allows team-patient intersubjectivity, focusing not only on the diseases, but on who they are. The carried-out home visits provided moments of listening for the performance of care in accordance with the needs, perceived and not perceived by the user. In the practice of STP, comprehensive care is provided, focused on the individual, showing the potential in continuing education and interprofessional teamwork, sharing knowledge, improving the sustainability of care and, consequently, qualifying health care, corroborating the results obtained in this study. Conclusion: Participation in the program allowed experiences that were previously absent during undergraduate school, such as contact with different health courses, practice in real SUS scenarios and application of concepts seen only in theory, such as humanized care and a comprehensive view, as well as communication with the team and the patient.
G. H. Mead and the value of his theory for education
Authors: Vitorino Artur José da Renda , Pires Pedro Serafim Anes
Published: March 2022

RESUMO Neste artigo são apresentados a teoria psicossocial e pragmatista erigida pelo filósofo americano George Herbert Mead (1863-1931) e seu valor para a educação escolar no presente. Mediante uma detalhada análise da obra, com apoio de comentadores, Mead é situado no contexto acadêmico de sua época, delineando-se os preceitos de seu pensamento e a idiossincrasia entre seus pares. Seu legado consiste num arcabouço teórico multidisciplinar - tratando-se de psicologia, sociologia e educação, interligados -, em uma linguagem clara e acessível, tendo sempre como princípio norteador a mediação e a construção do self, por intermédio da interação simbólica. O presente trabalho também se baseou em comentadores brasileiros e estrangeiros que estudaram Mead, mas oferece um enfoque diferente de como as ideias do filósofo poderiam ser úteis em elucidar questões pertinentes à educação escolar, tais como inclusão, bullying e papel social da escola.
Analytical sensitivity in education: the power of (auto)biographies in a training-research process involving beginner teachers
Authors: Pessoa Amanda Raquel Rodrigues , Cavalcante Maria Mikaele da Silva , Oliveira Wenderson Silva , Farias Isabel Maria Sabino de
Published: March 2022

RESUMO Este ensaio discute a perspectiva (auto)biográfica como sensibilidade analítica em pesquisas com formação docente e desenvolvimento profissional, direcionando-se, especialmente, à pesquisa com professoras/es iniciantes na carreira. O debate que levantamos, fruto das discussões em nosso grupo de pesquisa, centra-se na dimensão sensível a fim de trazer contribuições para se refletir a respeito das experiências-vividas e de suas escritas, atravessadas pelos cotidianos escolares. Nessa direção, nossa defesa parte de reconhecer narrativas (auto)biográficas e suas “escrevivências” como ferramentas potentes para a compreensão dos primeiros anos da docência e de processos de indução.
Educação profissional paulista e relações público-privadas na política curricular: Centro “Paula Souza” (1995-2018)
Authors: Moraes Carmen Sylvia Vidigal , Reis Elydimara Durso dos , Alencar Felipe
Published: March 2022

RESUMO O trabalho objetiva analisar as relações entre o público e o privado nas políticas de educação profissional do estado de São Paulo e, mais especificamente, nas propostas de organização curricular do ensino técnico de nível médio desenvolvido no Centro Estadual de Educação Tecnológica Paula Souza, nos anos 1995-2018. O estudo utiliza fontes primárias (documentos governamentais e os produzidos na instituição) e secundárias (revisão bibliográfica). Observa-se, nesse período histórico de gestão neoliberal dos governos do Partido da Social Democracia Brasileira no estado, a crescente atuação de agentes privados na formulação, no monitoramento e na avaliação das políticas curriculares da instituição. Tal movimento caracteriza um processo peculiar de privatização endógena do ensino profissional no âmbito da rede pública estadual de educação.
G. H. Mead e o valor de sua teoria para a educação
Authors: Vitorino Artur José da Renda , Pires Pedro Serafim Anes
Published: March 2022

RESUMO Neste artigo são apresentados a teoria psicossocial e pragmatista erigida pelo filósofo americano George Herbert Mead (1863-1931) e seu valor para a educação escolar no presente. Mediante uma detalhada análise da obra, com apoio de comentadores, Mead é situado no contexto acadêmico de sua época, delineando-se os preceitos de seu pensamento e a idiossincrasia entre seus pares. Seu legado consiste num arcabouço teórico multidisciplinar - tratando-se de psicologia, sociologia e educação, interligados -, em uma linguagem clara e acessível, tendo sempre como princípio norteador a mediação e a construção do self, por intermédio da interação simbólica. O presente trabalho também se baseou em comentadores brasileiros e estrangeiros que estudaram Mead, mas oferece um enfoque diferente de como as ideias do filósofo poderiam ser úteis em elucidar questões pertinentes à educação escolar, tais como inclusão, bullying e papel social da escola.
A construção social de uma escola quilombola: a experiência da Comunidade Caveira, RJ
Authors: Soares David Gonçalves , Maroun Kalyla , Soares Antonio Jorge Gonçalves
Published: March 2022

RESUMO O artigo tem por objetivo descrever e analisar o processo de construção social de uma escola quilombola e seu currículo, identificando atores sociais, processos de agenciamento e disputas de sentido sobre Educação Escolar Quilombola. Apresenta um estudo de caso da Escola Municipal Quilombola Dona Rosa Geralda, no município de São Pedro da Aldeia, Rio de Janeiro, com observações etnográficas e entrevistas realizadas na escola, na comunidade e na Secretaria Municipal de Educação entre os anos de 2017 e 2019. Entre os resultados, destacam-se: a presença de um forte sentido atribuído à escola enquanto agenciadora da identidade comunitária; a apropriação por seus profissionais de elementos discursivos associados aos movimentos negros enquanto resoluções curriculares disponíveis de tradução das políticas de educação quilombola; e a existência de mediações e dilemas que compõem essa experiência e que podem fornecer elementos para uma agenda de pesquisa.
A Novel Soluble ACE2 Protein Provides Lung and Kidney Protection in Mice Susceptible to Lethal SARS-CoV-2 Infection
Authors: Hassler Luise , Wysocki Jan , Gelarden Ian , Sharma Isha , Tomatsidou Anastasia , Ye Minghao , Gula Haley , Nicoleascu Vlad , Randall Glenn , Pshenychnyi Sergii , Khurram Nigar , Kanwar Yashpal , Missiakas Dominique , Henkin Jack , Yeldandi Anjana , Batlle Daniel
Published: March 2022

Significance Statement

A novel soluble angiotensin converting enzyme 2 (ACE2) protein with increased binding to the spike protein of the coronavirus that causes coronavirus disease 2019 (COVID-19) is protective in an animal model of severe COVID-19 , providing a proof of concept of efficacy. Administration of soluble ACE2 protein resulted in conversion of a lethal disease into a mild one that is reversible, with improved lung and kidney injury.

Background

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) uses full-length angiotensin converting enzyme 2 (ACE2) as a main receptor to enter target cells. The goal of this study was to demonstrate the preclinical efficacy of a novel soluble ACE2 protein with increased duration of action and binding capacity in a lethal mouse model of COVID-19.

Methods

A human soluble ACE2 variant fused with an albumin binding domain (ABD) was linked via a dimerization motif hinge-like 4-cysteine dodecapeptide (DDC) to improve binding capacity to SARS-CoV-2. This novel soluble ACE2 protein (ACE2–1-618-DDC-ABD) was then administered intranasally and intraperitoneally to mice before intranasal inoculation of SARS-CoV-2 and then for two additional days post viral inoculation.

Results

Untreated animals became severely ill, and all had to be humanely euthanized by day 6 or 7 and had pulmonary alveolar hemorrhage with mononuclear infiltrates. In contrast, all but one mouse infected with a lethal dose of SARS-CoV-2 that received ACE2–1-618-DDC-ABD survived. In the animals inoculated with SARS-CoV-2 that were untreated, viral titers were high in the lungs and brain, but viral titers were absent in the kidneys. Some untreated animals, however, had variable degrees of kidney proximal tubular injury as shown by attenuation of the proximal tubular brush border and increased NGAL and TUNEL staining. Viral titers in the lung and brain were reduced or nondetectable in mice that received ACE2–1-618-DDC-ABD, and the animals developed only moderate disease as assessed by a near-normal clinical score, minimal weight loss, and improved lung and kidney injury.

Conclusions

This study demonstrates the preclinical efficacy of a novel soluble ACE2 protein, termed ACE2–1-618-DDC-ABD, in a lethal mouse model of SARS-CoV-2 infection that develops severe lung injury and variable degrees of moderate kidney proximal tubular injury.
Investigating Animation-Based Achievement Tests According to Various Variables
Authors: GÜVEN DEMİR Elif , ÖKSÜZ Yücel
Published: March 2022

This research aimed to investigate animation-based achievement tests according to the item format, psychometric features, students’ performance, and gender. The study sample consisted of 52 fifth-grade students in Samsun/Turkey in 2017-2018. Measures of the research were open-ended (OE), animation-based open-ended (AOE), multiple-choice (MC), and animation-based multiple-choice (AMC) achievement tests developed for the unit “Motions of the Earth” of the subject area “Earth and Universe” of the science course. Tests were compared to item difficulty, item discrimination, internal consistency levels, and students’ performance and gender. Additionally, predicting students’ science grades was tested by animation-based open-ended and multiple-choice tests. Paired sample t-tests, Pearson’s correlation coefficient, Friedman test, Wilcoxon test, two-way mixed ANOVA tests, and multiple regression analysis were applied to analyze research data. Research results show no significant difference between animation-based and traditional tests' validity and reliability levels. Research result also shows that students’ test scores were significantly higher at animation-based tests than traditional tests. Besides, students’ test scores differed according to the animation type used in tests. However, gender was not a significant variable on students' test scores. Finally, despite using animation as a significant predictor of Science grades, just animation based multiple-choice test has significantly predicted students’ Science grades. Future research can investigate variables that affect students' participation in animation-based tests and their opinions on animation-based tests. The finding regarding the animation type variable can be investigated in-depth in terms of the effect levels of the visual and auditory elements added to the animations by including an equal number of items on the test.
Total High Molecular Weight DNA Extraction from plant tissues for Long Read Sequencing v1
Authors: Rai Subash
Published: March 2022

This protocol is a combination of two published protocols (10.1186/1746-4811-8-26 and 10.2144/000114460) with modifications and was developed as a research within GIH collaborative projects. Using this protocol, high quality High Molecular Weight (HMW) DNA (50kb – 300kb) was obtained from a wide range of plants tissues – Neptunia (leaves & roots), Senecio (leaves) and Mangoes (Kensington pride, Alphonso, and Tommy Atkins leaves). The DNA quality was assessed in Qubit, NanoDrop, TapeStation, PFGE and Oxford Nanopore Technologies. The yielded HMW DNA is suitable for long read sequencing technologies (ONT and PacBio) or similar experiments.
[RETRACTED] Mike Wolfe CBD Gummies Reviews - How It Works 2022? v1
Authors: not provided mikewolfereviews
Published: March 2022

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Mike Wolfe CBD Gummies Reviews - How It Works 2022?
[RETRACTED] Iron Maxxx Male Enhancement - Reviews, Price, benefits 2022 v1
Authors: not provided ironenhancementreviews
Published: March 2022

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Iron Maxxx Male Enhancement - Reviews, Price, benefits 2022
[RETRACTED] SlimFitGO Customer & My Honest Review Or Reviews - Does It Really Work! v1
Authors: not provided slimfitgo
Published: March 2022

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https://slimfitgo-official-reviews.jimdosite.com/ https://www.facebook.com/Slimfitgo-Reviews-105865745381137 https://www.facebook.com/Slimfitgo-Amazon-Reviews-102257969082106/ https://bumppy.com/tm/read-blog/25516_slimfitgo-natural-weight-loss-formula-reviews-real-or-hoax-safe-and-effective.html https://slimfitgo.clubeo.com/news/2022/03/03/is-slimfitgo-100-safe-to-use-7-natural-ingredients-does-is-real https://health-suplement-plan.blogspot.com/2022/03/slimfitgo-reviews-shocking-report.html https://groups.google.com/u/0/g/slimfitgo/c/4UeugUmEWTU https://groups.google.com/u/0/g/slimfitgo
E-Learning During the COVID-19 Outbreak: The Effect of the Grammar Translation Method and the Direct Method on Students’ English Competence
Authors: Kaharuddin
Published: March 2022

E-learning has been encouraged for usage during COVID-19, and this leaves problems with students’ learning activities, particularly in social and economic aspects. Applying appropriate teaching methods is expected, which motivates students to learn virtually. This paper studies the effect of the grammar translation method and the direct method on students’ English competence; the e-learning uses electronic tools with communication applications, such as Zoom and Google Classroom. The data were collected by questionnaires distributed to 100 students, of which 95 questionnaires were analyzable. The data were analyzed by path analysis via Microsoft SPSS version 21. The findings are as follows: 1) The grammar translation method has a significant direct effect on competence, contributing 27.7% to students’ English competence. The mediating role of Google Classroom has no contribution to the effect of the grammar translation method on students’ English competence. 2) The direct method has an indirect effect on students’ English competence, when utilized with the mediation of electronic tools that provide the Zoom application.
Factors Affecting the Behaviour in Integrating Google Meet Among Malaysian Primary ESL Teachers During Covid-19 Pandemic
Authors: Siang Winson Eng Wei , Mohamad Maslawati
Published: March 2022

The unsought Covid-19 pandemic has wreaked havoc in Malaysia’s education system, particularly at primary levels. Due to this, schools have been commanded to close immediately by the Ministry of Education (MOE) and Malaysian primary English as a Second Language (ESL) teachers have to conduct their teaching virtually via Google Meet under the government’s policy, where all teachers are provided with a Gmail address. The use of Google Meet is not well studied not only in primary schools, but also in the local environment. Therefore, this quantitative research was embarked to look into the factors that influence the integration of Google Meet among primary ESL teachers. Participants (n=123) were selected through purposive sampling based on their teaching option (TESL optionists and non-TESL optionists), teaching ESL in primary schools (n=12) located in Pasir Gudang, Johor, Malaysia. A 5-point Likert scale survey questionnaire adapted from previous studies was used. The factors of perceived usefulness and perceived ease of use towards the use of behaviour in integrating Google Meet were testified. The main findings in descriptive statistics indicated that majority of the primary ESL teachers have shown a positive attitude towards the use of Google Meet. However, some respondents remarkably noted that school administrators should render necessary assistance. The findings of this study could provide better insights for English teachers and school authority on the measures to integrate Google Meet in the teaching and learning of English at primary schools. If Google Meet is well integrated, pupils will benefit from this platform.
A Multimodal Discourse Analysis of Movie Posters From the Perspective of Visual Grammar — A Case Study of "Hi, Mom"
Authors: Peng Zhuyan
Published: March 2022

Poster is one of the main modes of multimodal discourse and plays a very important role in film promotion. Based on the visual grammar system under the framework of meta-functional theory, this paper gives a multimodal discourse analysis on a poster of the movie Hi, Mom. The purpose is to interpret the reproducing meaning, interactive meaning and composition meaning of the film poster, which helps to explore the connotation and thematic meaning of the film poster discourse, so as to help people better understand the theme of the film.
Scientific Story Writing Technique in the Alternative Assessment of the Subject of Liquids: The Sample of Science and Biology Teaching Students
Authors: Dolu Gamze , Ürek Handan , Bostan Sarıoğlan Ayberk
Published: March 2022

The present study aims to investigate university students’ conceptions of liquids with the help of the scientific story writing technique, which is one of the alternative assessment techniques. The study group comprised students studying science (n=50) and biology teaching departments (n=20) in Turkey. Thus, the study carries within two groups. In both groups, computer-supported teaching was carried out by the same researcher for two weeks regarding the subject of liquids. After this process, a scientific story writing technique applies to collect study data. In data analysis, utilized content analysis and descriptive statistics. Firstly, determined the statements involved in students’ stories. Afterward, ordered the statements under the categories ‘scientifically acceptable’, ‘partially acceptable,’ ‘scientifically unacceptable,’ and ‘not coded.’  The findings showed that the category with the highest percentage in the stories of both groups was the scientifically acceptable category. This result indicated that most of the students figured out the subject. However, I encountered several misconceptions also in students’ statements. Therefore, enrichment of learning methods is needed to eliminate student misunderstandings detected in learning.