bluuebunny/crossref_metadata_2025 huggingface dataset:
A 3D Carbon Architecture Encapsulation Strategy for Boosting the Performance of Nickel Disulfide as an Anode for Sodium-Ion Batteries
Authors: Li Yuzhu , Zhang Mengyuan , Zhang Boying , Li Bingke
Published: December 2024

Nickel disulfide (NiS2) nanoparticles are encapsulated within nitrogen and sulfur co-doped carbon nanosheets, which are grown onto carbon nanofibers to form an array structure (NiS2/C@CNF), resulting in a self-supporting film. This encapsulated structure not only prevents the agglomeration of NiS2 nanoparticles, but also memorably buffers its volume changes during charge/discharge cycles, thereby maintaining structural integrity. The nitrogen and sulfur co-doping enhances electronic conductivity and facilitates the faster ion transport of the carbon backbone, improving the low conductivity of the NiS2/C@CNF anodes. Consequently, the NiS2/C@CNF electrode exhibits a remarkable rate ability, reaching 55.4% of its capacity at 5 A g−1 compared to that at 0.1 A g−1, alongside an impressive cycling stability, with 89.9% capacity retention over 1500 cycles at 2 A g−1. This work underscores the efficacy of the 3D carbon backbone encapsulation strategy for enhancing the sodium storage property of transition metal-based anodes.
Heterologous Expression and Functional Verification of Extracellular Carbonic Anhydrases in Bacillus safensis yw6 from Mariana Trench
Authors: Wang Xinyu , Wang Pengna , Zhao Hancheng , He Yingying , Qu Changfeng , Miao Jinlai
Published: December 2024

The exploration and exploitation of deep-sea microbial resources is of great scientific value for understanding biological evolution under extreme conditions. Deep-sea microorganisms are critical in the ocean carbon cycle, and marine heterotrophic microorganisms secrete extracellular carbonic anhydrase (CA) to fix inorganic carbon, an important process in climate regulation. Extracellular CA provides a green method for fixing carbon dioxide into stable minerals containing Ca2+. However, studies on extracellular CA in deep-sea microorganisms are limited. In this study, Bacillus safensis yw6 was isolated from Mariana Trench sediments and three candidate extracellular CA genes (β-ca1, β-ca2, and γ-ca) were identified by whole genome sequencing. Bioinformatics analyses showed that these CAs have different structural compositions, with the β-CA having α-helix and random coiling, whereas the γ-CA has more random coiling and stretched strands. Heterologous expression in E. coli BL21 (DE3) showed that β-CA2 had the highest enzyme activity, followed by γ-CA and β-CA1. Field emission scanning electron microscopy (FESEM) observations showed that the engineered strains with β-ca2 genes produced deposits that were like those from natural sources. This finding not only provides new perspectives for the utilization of deep-sea microbial resources, but also provides an important scientific basis for the molecular mechanisms of extracellular CAs of deep-sea microbes.
Larvicidal and Oviposition Activity of Commercial Essential Oils of Abies sibirica Ledeb., Pogostemon cablin (Blanco) Benth., Juniperus communis L. and Their Combinations Against Aedes aegypti
Authors: de Aguiar Júlio César Ribeiro de Oliveira Farias , da Silva Ana Carla , Santos Eduarda Florêncio , Vieira Gilson José da Silva Gomes , Araújo Liderlanio de Almeida , de Andrade José Jorge Almeida , Anselmo Wevertton Marllon , da Rocha Suyana Karolyne Lino , dos Santos Fábio Henrique Galdino , Arruda Camila Caroline Lopes , Albuquerque Caroline Francisca de Oliveira , Claudino Libna Larissa Monteiro , da Silva Priscila Soares , Silva Danilo Gustavo Rodrigues , Aguiar João Vitor Castro , de Veras Bruno Oliveira , Navarro Daniela Maria do Amaral Ferraz
Published: December 2024

Aedes aegypti is a vector responsible for the transmission of various arboviruses and is considered by the World Health Organization to be one of the main public health problems in the world. This study evaluated the larvicidal and oviposition activity of essential oils from Abies sibirica, Pogostemon cablin and Juniperus communis and their formulations. Chromatographic analysis by GCMS identified a total of 28, 52 and 18 compounds for the oils of the species A. sibirica, J. communis and P. cablin, respectively. The larvicidal bioassays showed an LC50 of 67.53 ppm, 92.45 ppm and 35.95 ppm, respectively, for A. sibirica (A), J. communis (J) and P. cablin (P) as well as their binary (J + P, 39.50 ppm; A + P, 51.64 ppm) and ternary (A + J + P, 66.99 ppm) formulations. These oils and formulations also showed deterrent activity at the larvicidal concentrations tested (A. sibirica: OAI: −0.41; J. communis: OAI: −0.31; P. cablin: OAI: −0.62; A + J + P: −0.30; A + P: −0.68; A + J: −0.29; and J + P: −0.30). The oils and their formulations are a potential larvicidal source for mitigating the proliferation of diseases by this vector.
Advances in Nanocomposites: Preparation, Characterization, Properties, and Applications
Authors: Nambiar Reshma B , Perumal Anand Babu , Sadiku Emmanuel Rotimi
Published: December 2024

Nanocomposites are a class of nanomaterials wherein one or more phases, of a nano-sized dimension (zero dimensions, one dimension, and two dimensions), are embedded in ceramic, metal, or polymer materials, etc [...]
Extracellular Vesicles and PlantCrystals for Improved Bioavailability of Curcumin as a BCS Class IV Drug
Authors: Alkhaldi Muzn , Sehra Tehseen , Sengupta Soma , Keck Cornelia M.
Published: December 2024

The limited water solubility of active compounds remains a significant challenge for efficient dermal drug delivery, particularly for BCS class IV drugs such as curcumin. This study aimed to enhance curcumin’s dermal penetration using two strategies: extracellular vesicles (EVs) and plantCrystals derived from soybeans. EVs were isolated using classical methods. However, plantCrystals containing extracellular vesicles (PCEVs) were formed during the preparation of plantCrystals through bead milling. Curcumin was either added after PCEVs were formed, resulting in curcumin-added PCEVs, or added to the soybean dispersion before bead milling, forming curcumin-loaded PCEVs. The formulations were characterized for their physicochemical properties and assessed for dermal penetration efficacy using quantitative dermatokinetic and semi-quantitative ex vivo porcine ear models. The results indicated that curcumin-loaded PCEVs achieved higher penetration efficacy compared to curcumin-added PCEVs and curcumin-loaded EVs, with approximately 1.5-fold and 2.7-fold increases in penetration efficacy, respectively. Additionally, curcumin-loaded PCEVs showed superior penetration depth, while curcumin from the curcumin-loaded EVs remained in the stratum corneum. These findings suggest that the plantCrystals strategy via bead milling offers a more effective approach than the classical EVs strategy for improving the topical delivery of class IV drugs like curcumin.
Modelling the Impact of Argon Atoms on a WO3 Surface by Molecular Dynamics Simulations
Authors: Shermukhamedov Shokirbek , Maihom Thana , Probst Michael
Published: December 2024

Machine learning potential energy functions can drive the atomistic dynamics of molecules, clusters, and condensed phases. They are amongst the first examples that showed how quantum mechanics together with machine learning can predict chemical reactions as well as material properties and even lead to new materials. In this work, we study the behaviour of tungsten trioxide (WO3) surfaces upon particle impact by employing potential energy surfaces represented by neural networks. Besides being omnipresent on tungsten surfaces exposed to air, WO3 plays an important role in nuclear fusion experiments due to the preferred use of tungsten for plasma-facing components. In this instance, the formation of WO3 is caused by the omnipresent traces of oxygen. WO3 becomes a plasma-facing material, but its properties, especially concerning degradation, have hardly been studied. We employ molecular dynamics simulations to investigate sputtering, reflection, and adsorption phenomena occurring on WO3 surfaces irradiated with Argon. The machine-learned potential energy function underlying the MD simulations is modelled using a neural network (NNP) trained from large sets of density functional theory calculations by means of the Behler–Parrinello method. The analysis focuses on sputtering yields for both oxygen and tungsten (W), for various incident energies and impact angles. An increase in Ar incident energy increases the sputtering yield of oxygen, with distinct features observed in different energy ranges. The sputtering yields of tungsten remain exceedingly low, even compared to pristine W surfaces. The ratios between the reflection, adsorption, and retention of the Ar atoms have been analyzed on their dependence of impact energy and incident end angles. We find that the energy spectrum of sputtered oxygen atoms follows a lognormal distribution and offers information about surface binding energies on the WO3 surface.
Aged Garlic Extract (AGE) and Its Constituent S-Allyl-Cysteine (SAC) Inhibit the Expression of Pro-Inflammatory Genes Induced in Bronchial Epithelial IB3-1 Cells by Exposure to the SARS-CoV-2 Spike Protein and the BNT162b2 Vaccine
Authors: Gasparello Jessica , Papi Chiara , Marzaro Giovanni , Macone Alberto , Zurlo Matteo , Finotti Alessia , Agostinelli Enzo , Gambari Roberto
Published: December 2024

Garlic (Allium sativum L.) is a species of the onion family (Alliaceae) widely used as a food and a folk medicine. The objective of this study was to determine the effects of AGE (aged garlic extract) on pro-inflammatory genes relevant to COVID-19. To this aim, we treated bronchial epithelial IB3-1 cells with SARS-CoV-2 spike protein (S-protein) or with the COVID-19 BNT162b2 vaccine in the absence or in the presence of AGE. The results obtained demonstrated that AGE is a potent inhibitor of the S-protein-induced expression of the IL-1β, IL-6 and IL-8 genes. Bio-Plex analysis demonstrated that AGE reduced release of IL-6 and IL-8, which were highly induced by S-protein. No inhibition of cells’ growth, toxicity and pro-apoptotic effects were found in AGE-treated cells. The effects of one of the major AGE constituents (S-allyl cysteine, SAC) were studied on the same experimental model systems. SAC was able to inhibit the S-protein-induced expression of IL-1β, IL-6 and IL-8 genes and extracellular release of IL-6 and IL-8, confirming that S-allyl-cysteine is one of the constituents of AGE that is responsible for inhibiting S-protein-induced pro-inflammatory genes. Docking experiments suggest that a possible mechanism of action of SAC is an interference with the activity of Toll-like receptors (TLRs), particularly TLR4, thereby inhibiting NF-κB- and NF-κB-regulated genes, such as IL-1β, IL-6 and IL-8 genes. These results suggest that both AGE and SAC deserve further experimental efforts to verify their effects on pro-inflammatory genes in SARS-CoV-2-infected cells.
Vitamin C and MEK Inhibitor PD0325901 Synergistically Promote Oligodendrocytes Generation by Promoting DNA Demethylation
Authors: Ren Xinyue , Yang Ying , Wang Min , Yuan Qianting , Suo Na , Xie Xin
Published: December 2024

DNA methylation and demethylation are key epigenetic events that regulate gene expression and cell fate. DNA demethylation via oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) is typically mediated by TET (ten-eleven translocation) enzymes. The 5hmC modification is considered an intermediate state of DNA demethylation; it is particularly prevalent in the brain and is believed to play a role in the development of many cell types in the brain. Our previous studies have identified that vitamin C (Vc) and MEK inhibitor PD0325901 could significantly promote OPC (oligodendrocyte progenitor cell)-to-OL (oligodendrocyte) differentiation. Here we discovered that Vc and PD0325901 may promote OPC-to-OL differentiation by inducing DNA demethylation via hydroxymethylation. Blocking 5hmC formation almost totally blocked Vc- and PD0325901-stimulated OPC-to-OL differentiation. In addition, TET1 is not involved in Vc,- and PD0325901-promoted OL generation. We also found a synergistic effect between the two compounds in inducing OL generation, suggesting the possibility of a combination therapy for demyelination diseases in the future.
Fluorescently Tagged Poly(methyl methacrylate)s
Authors: Grisi Fabia , Troiano Rubina , Fiore Donatella , Gazzerro Patrizia , Lettieri Mariateresa , Venditto Vincenzo , Pragliola Stefania
Published: December 2024

Plastic pollution is a global problem affecting the environment and, consequently, people’s well-being. Careful and timely end-of-life plastic recycling is certainly a way, albeit a partial one, to remedy the problem. The immediate identification and selection of the different types of plastic materials in the recycling process certainly facilitate its recovery and reuse, allowing the damage caused by plastic emission into the environment to be limited. Recently, new technologies for automatic sorting of plastics based upon fluorescent tagging have been considered. This article reports the synthesis and characterization of fluorescent copolymers of poly(methyl methacrylate) (PMMA) that could be potentially used as fluorescent markers of commercial PMMA. Poly(methylmetacrylate-co-2-(9-carbazolyl)ethyl methacrylate) (P(MMA-co-CEMA)) and poly(methylmetacrylate-co-7-methacryloyloxycoumarin) (P(MMA-co-MAOC)) samples containing a small number of fluorescent units (<4%) were synthesized by free-radical polymerization. All copolymer samples show chemico-physical properties like those of pure PMMA and produce fluorescence emission under 290 nm wavelength excitation. P(MMA-co-CEMA)s and P(MMA-co-MAOC)s were also tested as fluorescent dyes for PMMA identification. The experimental results demonstrate that PMMA/P(MMA-co-CEMA) and PMMA/P(MMA-co-MAOC) blends prepared using 1% by weight of fluorescent copolymer show a homogeneous morphology completely similar to pure PMMA and are still optically active.
Harshly Oxidized Activated Charcoal Enhances Protein Persulfidation with Implications for Neurodegeneration as Exemplified by Friedreich’s Ataxia
Authors: Vo Anh T. T. , Khan Uffaf , Liopo Anton V. , Mouli Karthik , Olson Kenneth R. , McHugh Emily A. , Tour James M. , Pooparayil Manoj Madhavan , Derry Paul J. , Kent Thomas A.
Published: December 2024

Harsh acid oxidation of activated charcoal transforms an insoluble carbon-rich source into water-soluble, disc structures of graphene decorated with multiple oxygen-containing functionalities. We term these pleiotropic nano-enzymes as “pleozymes”. A broad redox potential spans many crucial redox reactions including the oxidation of hydrogen sulfide (H2S) to polysulfides and thiosulfate, dismutation of the superoxide radical (O2−*), and oxidation of NADH to NAD+. The oxidation of H2S is predicted to enhance protein persulfidation—the attachment of sulfur to cysteine residues. Persulfidated proteins act as redox intermediates, and persulfidation protects proteins from irreversible oxidation and ubiquitination, providing an important means of signaling. Protein persulfidation is believed to decline in several neurological disorders and aging. Importantly, and consistent with the role of persulfidation in signaling, the master antioxidant transcription factor Nrf2 is regulated by Keap1’s persulfidation. Here, we demonstrate that pleozymes increased overall protein persulfidation in cells from apparently healthy individuals and from individuals with the mitochondrial protein mutation responsible for Friedreich’s ataxia. We further find that pleozymes specifically enhanced Keap1 persulfidation, with subsequent increased accumulation of Nrf2 and Nrf2’s antioxidant targets.
Lactoferrin as a Versatile Agent in Nanoparticle Applications: From Therapeutics to Agriculture
Authors: Akdaşçi Emir , Eker Furkan , Duman Hatice , Singh Priyanka , Bechelany Mikhael , Karav Sercan
Published: December 2024

Nanoparticles (NPs) have emerged as a potent choice for various applications, from drug delivery to agricultural studies, serving as an alternative and promising methodology for future advancements. They have been widely explored in delivery systems, demonstrating immense promise and high efficiency for the delivery of numerous biomolecules such as proteins and anticancer agents, either solely or modified with other compounds to enhance their capabilities. In addition, the utilization of NPs extends to antimicrobial studies, where they are used to develop novel antibacterial, antifungal, and antiviral formulations with advanced characteristics. Lactoferrin (Lf) is a glycoprotein recognized for its significant multifunctional properties, such as antimicrobial, antioxidant, anti-inflammatory, anticancer, and neuroprotective effects. Its activity has a broad distribution in the human body, with Lf receptors present in multiple regions. Current research shows that Lf is utilized in NP technology as a surface material, encapsulated biomolecule, and even as an NP itself. Due to the abundance of Lf receptors in various regions, Lf can be employed as a surface material in NPs for targeted delivery strategies, particularly in crossing the BBB and targeting specific cancers. Furthermore, Lf can be synthesized in an NP structure, positioning it as a strong candidate in future NP-related applications. In this article, we explore the highlighted and underexplored areas of Lf applications in NPs research.
Deciphering Surface-Localized Structure of Nanodiamonds
Authors: Ma Li , He Zhijie , Chen Keyuan , Li Hanqing , Wu Yongzhi , Ye Jueyi , Hou Hongying , Rong Ju , Yu Xiaohua
Published: December 2024

Nanomaterials, heralded as the “new materials of the 21st century” for their remarkable physical and chemical properties and broad application potential, have attracted substantial attention in recent years. Among these materials, which challenge traditional physical boundaries, nanodiamonds (NDs) are widely applied across diverse industries due to their exceptional surface multifunctionality and chemical stability. Nevertheless, atomic-level manipulation of NDs presents considerable challenges, which require detailed structural analysis to thoroughly elucidate their properties. This study utilizes density functional theory (DFT), lattice dynamics, and molecular dynamics (MD) simulations to analyze the structural and property characteristics of NDs. Fine structural analysis reveals that, despite variations in particle size, surface layer thickness remains relatively constant at approximately 3 Å. DFT methods enable computation of the surface layer to capture subtle electronic characteristics, while the internal core is analyzed via MD. Further investigation into amorphous structure control indicates that ND surface amorphous structures with a packing coefficient above 0.38 are thermodynamically stable. This study offers a novel approach to nanomaterial control in practical applications by elucidating the core–shell interactions and surface structures of NDs.
Palmitoylethanolamide in Postmenopausal Metabolic Syndrome: Current Evidence and Clinical Perspectives
Authors: Medoro Alessandro , Davinelli Sergio , Fogacci Federica , Alfieri Stefania , Tiso Domenico , Cicero Arrigo F. G. , Scapagnini Giovanni
Published: December 2024

Menopause leads to a decline in estrogen levels, resulting in significant metabolic alterations that increase the risk of developing metabolic syndrome—a cluster of conditions including central obesity, insulin resistance, dyslipidemia, and hypertension. Traditional interventions such as hormone replacement therapy carry potential adverse effects, and lifestyle modifications alone may not suffice for all women. This review explores the potential role of palmitoylethanolamide (PEA), an endogenous fatty acid amide, in managing metabolic syndrome during the postmenopausal period. PEA primarily acts by activating peroxisome proliferator-activated receptor-alpha (PPAR-α), influencing lipid metabolism, energy homeostasis, and inflammation. Evidence indicates that PEA may promote the browning of white adipocytes, enhancing energy expenditure and reducing adiposity. It also improves lipid profiles by boosting fatty acid oxidation and decreasing lipid synthesis, potentially lowering low-density lipoprotein cholesterol and triglyceride levels while increasing high-density lipoprotein cholesterol. Additionally, the anti-inflammatory properties of PEA enhance insulin sensitivity by reducing pro-inflammatory cytokines that interfere with insulin signaling. PEA may aid in weight management by influencing appetite regulation and improving leptin sensitivity. Furthermore, its neuroprotective effects may address the mood disturbances and cognitive decline associated with menopause. Given these multifaceted biological activities and a favorable safety profile, PEA may represent a promising non-pharmacological supplement for managing metabolic syndrome in postmenopausal women. However, further large-scale clinical studies are necessary to establish its efficacy, optimal dosing, and long-term safety. If validated, PEA could become an integral part of strategies to improve metabolic and neuropsychological health outcomes in this population.
Effects of Illumination Color on Hypothalamic Appetite-Regulating Gene Expression and Glycolipid Metabolism
Authors: Wang Qi , Li Qianru , Quan Tuo , Liang Hongshan , Li Jing , Li Kaikai , Ye Shuxin , Zhu Sijia , Li Bin
Published: December 2024

Irregular illumination is a newly discovered ambient factor that affects dietary and metabolic processes. However, the effect of the modulation of long-term light exposure on appetite and metabolism remains elusive. Therefore, in this current study, we systematically investigated the effects of up to 8 weeks of exposure to red (RL), green (GL), and white light (WL) environments on appetite, food preferences, and glucose homeostasis in mice on both high-fat and low-fat dietary patterns. It was found that the RL group exacerbated high-fat-induced obesity in mice compared with GL- or WL-treated mice. RL-exposed mice exhibited worsened metabolic profiles, including impaired glucose tolerance/insulin sensitivity, elevated lipid levels, and reduced serum insulin levels. Serological analyses showed that RL exposure resulted in decreased leptin levels and increased levels of orexigenic and hunger hormones in mice. Further qPCR analysis showed that the expression levels of the hypothalamic appetite-related genes NPY and AgRP mRNA were upregulated in RL-treated mice, while the expression level of the appetite suppressor gene POMC mRNA was downregulated. The results of this study will be instructive for the regulation of appetite and metabolism from the perspective of illumination colors.
Effects of Different Processing on miRNA and Protein in Small Extracellular Vesicles of Goat Dairy Products
Authors: Fan Yuqin , Li Zhikang , Hou Yanmei , Tan Chumin , Xiong Sheng , Zhong Jinjing , Xie Qiuling
Published: December 2024

Objectives: Small extracellular vesicles (sEVs) are nanosized vesicles with biological activities that exist in milk, playing functional roles in immunity, gut balance, and the nervous system. Currently, little is known about the impact of processing on milk sEVs. Methods: In this study, sEVs were collected from raw goat milk (g-sEV), pasteurized goat milk (pg-sEV), and goat milk powder (p-sEV) using a sucrose cushion centrifugation combined with qEV chromatography. Then, the sEVs were identified and compared using NTA, Western blot, and TEM. After extracting RNA and the total proteome from sEVs derived from different samples, the RNA was subjected to high-throughput sequencing, and peptide fragments were analyzed using mass spectrometry. Finally, GO and KEGG pathway analyses were performed on the results. Results: The characterization results revealed a decrease in diameter as the level of processing increased. High-throughput sequencing results showed that all three types of small extracellular vesicles were found to be rich in miRNA, and no significant differences were observed in the most abundant sEV species. Comparing with g-sEV, there were 3938 and 4645 differentially expressed miRNAs in pg-sEV and p-sEV, respectively, with the majority of them (3837 and 3635) being downregulated. These differentially expressed miRNAs were found to affect biological processes or signaling pathways such as neurodevelopment, embryonic development, and transcription. Proteomic analysis showed that there were 339 differentially expressed proteins between g-sEV and pg-sEV, with 209 proteins being downregulated. Additionally, there were 425 differentially expressed proteins between g-sEV and p-sEV, with 293 proteins being downregulated. However, no significant differences were observed in the most abundant protein species among the three types of sEVs. Enrichment analysis indicated that the differentially expressed proteins were associated with inflammation, immunity, and other related processes. Conclusions: These results indicate that extracellular vesicles have a protective effect on their cargo, while processing steps can have an impact on the size and quantity of the sEVs. Furthermore, processing can also lead to the loss of immune-related miRNA and proteins in sEVs.
The Protective Role of Vitamin K in Aging and Age-Related Diseases
Authors: Kaźmierczak-Barańska Julia , Karwowski Bolesław T.
Published: December 2024

Aging is an inevitable aspect of life, but age-related diseases are not an inseparable part of the aging process, and their risk can be reduced through a healthy lifestyle. Vitamin K has a broader impact than just blood clotting, and yet it remains overshadowed by other vitamins and underestimated by both doctors and consumers. Vitamin K (VK) is a multifunctional micronutrient with anti-inflammatory and antioxidant properties, whose deficiency may cause age-related diseases such as cardiovascular diseases, neurodegenerative diseases and osteoporosis. There is a growing body of evidence supporting the role of vitamin K as a protective nutrient in aging and inflammation. This review summarizes the current knowledge regarding the molecular aspects of the protective role of vitamin K in aging and age-related diseases and its clinical implications.
Investigating the Effects of Microclimate on Arboviral Kinetics in Aedes aegypti
Authors: Turner Erik A. , Clark Samantha D. , Peña-García Víctor Hugo , Christofferson Rebecca C.
Published: December 2024

Aedes aegypti are indoor-dwelling vectors of many arboviruses, including Zika (ZIKV) and chikungunya (CHIKV). The dynamics of these viruses within the mosquito are known to be temperature-dependent, and models that address risk and predictions of the transmission efficiency and patterns typically use meteorological temperature data. These data do not differentiate the temperatures experienced by mosquitoes in different microclimates, such as indoor vs. outdoor. Using temperature data collected from Neiva Colombia, we investigated the impact of two microclimate temperature profiles on ZIKV and CHIKV infection dynamics in Ae. aegypti. We found that the vector mortality was not significantly impacted by the difference in temperature profiles. Further, we found that the infection and dissemination rates were largely unaffected, with only ZIKV experiencing a significant increase in infection at outdoor temperatures at 21 days post-infection (dpi). Further, there was a significant increase in viral titers in the abdomens of ZIKV-infected mosquitoes at 21 dpi. With CHIKV, there was a significant titer difference in the abdomens of mosquitoes at both 7 and 14 dpi. While there were differences in vector infection kinetics that were not statistically significant, we developed a simple stochastic SEIR-SEI model to determine if the observed differences might translate to notable differences in simulated outbreaks. With ZIKV, while the probability of secondary transmission was high (>90%) under both microenvironmental scenarios, there was often only one secondary case. However, CHIKV differences between microenvironments were more prominent. With over 90% probability of secondary transmission, at indoor conditions, the peak of transmission was higher (over 850 cases) compared to the outdoor conditions (<350 cases). Further, the time-to-peak for indoor was 130 days compared to 217 days for outdoor scenarios. Further investigations into microenvironmental conditions, including temperature, may be key to increasing our understanding of the nuances of CHIKV and ZIKV vectorial capacity, epidemiology, and risk assessment, especially as it affects other aspects of transmission, such as biting rate. Overall, it is critical to understand the variability of how extrinsic factors affect transmission systems, and these data add to the growing catalog of knowledge of how temperature affects arboviral systems.
Chandipura Virus Causing Large Viral Encephalitis Outbreaks in India
Authors: Brisse Morgan E. , Ly Hinh
Published: December 2024

Chandipura virus (CHPV) is a negative-, single-stranded RNA virus belonging to the family Rhabdoviridae [...]
Novel Haemocystidium sp. Intraerythrocytic Parasite in the Flatback (Natator depressus) and Green (Chelonia mydas) Turtle in Western Australia
Authors: Young Erina J. , Vaughan-Higgins Rebecca , Warren Kristin S. , Whiting Scott D. , Rossi Gabriele , Stephens Nahiid S. , Yeap Lian , Austen Jill M.
Published: December 2024

Malaria and other haemosporidian parasites are common in reptiles. During baseline health surveys of sea turtles in Western Australia (WA), haemosporidian parasites were detected in flatback (Natator depressus) and green (Chelonia mydas) turtle erythrocytes during routine blood film examination. 130 blood samples were screened via polymerase chain reaction (PCR), including 105 N. depressus, 20 C. mydas, and 5 olive ridley turtles (Lepidochelys olivacea). A novel Haemocystidium sp. was identified, detected exclusively in foraging turtles and not in nesting turtles. The combined prevalence by microscopic and molecular methods was 16.9% (22/130), primarily affecting immature C. mydas (77.3%; 17/22). Mature N. depressus were also affected (22.7%; 5/22). DNA sequencing of a partial fragment of the mitochondrial cytochrome b (cytb) gene together with phylogenetic analysis identified two different Haemocystidium sp. genotypes, A and B, with genotype A being most prevalent. The phylogenetic analysis showed close genetic relationships to Haemocystidium sp. in freshwater and terrestrial turtles, suggesting a shared evolutionary lineage despite ecological differences. Preliminary analysis indicates that this parasite is incidental, as no association between health and parasite presence or grade was detected. This study provides the first formal detection of haemosporidian parasites in sea turtles, contributing essential baseline data while highlighting their evolutionary significance and host–parasite ecological relationships.
Repurposing Dapagliflozin for Mitigation of the Kidney Injury Triggered by Cadmium in Rats: Role of Autophagy, Apoptosis, and the SIRT1/Nrf2/HO-1 Pathway
Authors: Arab Hany H. , Althobaiti Musaad M. , Alharthi Abdulaziz S. , Almalki Emad O. , Alsoubie Saif S. , Qattan Jawad M. , Almalki Saeed A. , Ashour Ahmed M. , Eid Ahmed H.
Published: December 2024

Background/Objectives: The antioxidant/antiapoptotic features of dapagliflozin (DPG) have mediated its beneficial actions against several experimental models. However, no studies have been conducted to determine whether DPG mitigates the renal injury triggered by cadmium (Cd). Herein, DPG was studied for its potential to attenuate kidney damage in Cd-intoxicated rats, as well as to unravel the mechanisms involving oxidative events, autophagy, and apoptosis. Methods: Histopathological analysis, immunohistochemical staining, and ELISA were conducted on kidney tissue samples. Results: Cd administration (5 mg/kg/day; p.o.) prompted significant renal damage, as evidenced by histopathological changes, elevated kidney injury molecule-1 (KIM-1) expression, and increased serum creatinine and urea. Interestingly, DPG (1 mg/kg/day; p.o.) significantly mitigated these harmful effects without affecting renal Cd metal accumulation. Mechanistically, DPG curbed Cd-induced renal pro-oxidant response and stimulated the antioxidant sirtuin 1 (SIRT1)/nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase 1 (HO-1) axis. Moreover, DPG restored autophagy by decreasing sequestosome-1/protein 62 (SQSTM-1/p62) accumulation and stimulating the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin (mTOR) pathway. In tandem, DPG suppressed Cd-induced apoptosis by lowering renal Bcl-2 associated-x protein (Bax) and cytochrome C (Cyt C) levels and caspase 3 activity. Conclusions: These findings indicate that DPG attenuates Cd-induced nephrotoxicity by enhancing the SIRT1/Nrf2/HO-1 antioxidant pathway, promoting AMPK/mTOR-directed autophagy, and inhibiting apoptotic cell death.
Nerelimomab Alleviates Capsaicin-Induced Acute Lung Injury by Inhibiting TNF Signaling and Apoptosis
Authors: Huang Lijuan , Du Bing , Cui Xiaohu , Zhao Hanqing , Feng Yanlin , Xu Ziying , Long Jianhai , Yuan Jing , You Fuping
Published: December 2024

Background: Capsaicin is commonly used as a flavoring and a riot control agent. However, long-term exposure or high doses can cause acute lung injury in military and police personnel. The mechanisms underlying capsaicin-induced pulmonary toxicity remain unclear. Therefore, this study investigated the molecular mechanisms involved in capsaicin-induced acute lung injury using C57BL/6N mice. Methods: Through both transcriptomic and proteomic analyses of mouse lung tissue, we identified the involvement of the TNF signaling pathway in capsaicin-mediated acute lung injury. Next, we explored the role of TNF signaling in the progression of acute lung injury to identify potential therapeutic targets. In a capsaicin-induced acute lung injury mouse model and A549 cells, we assessed the therapeutic potential of the TNF-α antibody Nerelimomab. Compared with the control group, TNF-α up-regulation was observed, which correlated with increased pathological changes and elevated IL-6 (p < 0.01) and IL-18 (p < 0.01) levels, both in vivo and in vitro. Results: Flow cytometry revealed that compared to the capsaicin group, Nerelimomab treatment reduced the number of capsaicin-induced apoptotic cells (p < 0.001) and was associated with an increased Bcl-2/Bax ratio (p < 0.01) and reduced cleaved caspase 3 expression (p < 0.001). Analysis of A549 cells treated with capsaicin and Nerelimomab corroborated these results. These findings confirm the involvement of the TNF signaling pathway in capsaicin-induced acute lung injury and the apoptosis of alveolar epithelial cells. Conclusions: In conclusion, capsaicin inhalation can cause acute lung injury, and targeting the TNF signaling pathway offers a promising therapeutic strategy. Nerelimomab demonstrates significant potential in alleviating acute lung injury by inhibiting inflammatory mediator release and diminishing apoptosis. Based on transcriptomic and proteomic analyses, this study highlights the crucial role of the TNF signaling pathway in capsaicin-induced acute lung injury and supports the therapeutic efficacy of Nerelimomab in reducing epithelial apoptosis.
Quality by Design Approach for the Formulation and Evaluation of Stem Cells Derived Rosmarinic Acid-Loaded Nanofibers as an Anti-Wrinkle Patch: In Vitro and In Vivo Characterizations
Authors: Abdelmonem Rehab , Bakr Ahmed , Badawy Ingy , Abd El Maksoud Ahmed Ibrahim , Attia Reem T.
Published: December 2024

Background/Objectives: Skin wrinkles result from a myriad of multifaceted processes involving intrinsic and extrinsic aging. To combat this effect, plant stem cells offer a renewable and eco-friendly source for various industries, including cosmeceuticals. Salvia miltiorrhiza (SM), which contains the bioactive compound Rosmarinic acid (RA) and has been proposed for its anti-wrinkle effect. Methods: In the present study, calli from SM were cultured and Quality by Design (QbD) was implemented to investigate the effect of different types and concentrations of elicitors; jasmonic acid (JA) and salicylic acid (SA). Both raised RA levels yet, jasmonic acid (50 µM) has resulted in the highest yield for RA, at 16 mg/g. A nanofiber patch was prepared and characterized in-vitro by the release percentage, drug content, swelling degree, scanning electron microscope, and surface roughness. Then, the anti-wrinkle effect of the patch was tested in a UV wrinkle-induced mouse model. Results: Interestingly, after treatment, there were visibly fewer wrinkles, and the skin was softer than in the untreated control group. This study suggests that the treatment exerted its effect through the Nrf2/Keap1 pathway, which plays a crucial role in cellular antioxidant protective processes. By activating this pathway through boosting Nrf2 and diminishing Keap1 cellular content, the nanofiber patch enhances the production of antioxidant enzymes, such as superoxide dismutase and glutathione peroxidase, enhancesglutathione, and reduces the skin lipid peroxidation, collectively indicating enhanced skin quality. Conclusions: In conclusion, this study highlights the importance of this formula as an anti-wrinkle treatment, and future clinical studies are recommended to further unveil the potential of this formula.
Antimicrobial Preservatives in Cyclodextrin-Containing Drug Formulations
Authors: Jansook Phatsawee , Sigurdsson Hákon Hrafn , Pilotaz Frédéric , Loftsson Thorsteinn
Published: December 2024

In general, antimicrobial preservatives are essential components of multidose pharmaceutical formulations to prevent microbial growth and contamination, many of which contain lipophilic and poorly water-soluble drugs in need of solubilizing excipients, such as cyclodextrins (CDs). However, CDs frequently reduce or even abolish the antimicrobial activities of commonly used pharmaceutical preservatives. The degree of inactivation depends on the CD complexation of the preservatives, which in turn depends on their chemical structure and physiochemical properties. In general, lipophilic preservatives are more likely to be inactivated; however, CDs are also known to inactivate highly water-soluble preservatives. In some drug formulations, preservative inactivation has been offset by including excipients that possess some antimicrobial activity on their own. In this review, we explain how CDs interact with some commonly used pharmaceutical preservatives and why some preservatives are more susceptible to CD inactivation than others are.
Design Considerations for 1.6 Tbit/s Data Center Interconnects: Evaluating IM/DD and Coherent Transmission over O-Band Transmission Window
Authors: Juarez Adrian A. , Zhu Yanjun , Chen Xin , Li Ming-Jun
Published: December 2024

As data center interconnects surge towards a 1.6 Tbit/s data rate, achieving cost-effective and technically viable solutions present challenges. Intensity-modulation and direct-detection (IM/DD) transmission over O-Band using standard single-mode fiber has emerged as a promising low-cost option. However, understanding the limitations imposed by factors like chromatic dispersion (CD) and fiber non-linearity (FWM) is crucial, particularly in different scenarios, such as operating at 8 × 100 GBaud PAM4 in an LWDM-8 configuration. In this paper, we adopt a statistical approach to assess outage probability and consider practical fluctuations in link parameters. Numerical modeling suggests IM/DD can span distances up to 5 km with transmission power under 0 dBm using this architecture. In addition, we evaluate recently proposed architecture to achieve 800 Gbit/s and 1.6 Tbit/s using an LWDM4 configuration and assess the impact of FWM to understand the role of zero-dispersion wavelength (ZDW) of the fiber. Coherent transmission leverages more powerful signal processing capabilities which extends the transmission range. Yet, reducing coherent transmission complexity is desirable for cost-effective and power-efficient data center applications. By exploring dual wavelength transmission and DP-16 QAM transceivers, akin to IM/DD counterparts, the feasibility of streamlining this architecture is also studied. The analysis indicates that the complexity of the coherent approach can be reduced without significant penalties for distances up to 10 km.
Effects of Far-Red Light and Ultraviolet Light-A on Growth, Photosynthesis, Transcriptome, and Metabolome of Mint (Mentha haplocalyx Briq.)
Authors: Yu Lishu , Bu Lijun , Li Dandan , Zhu Kaili , Zhang Yongxue , Wu Shaofang , Chang Liying , Ding Xiaotao , Jiang Yuping
Published: December 2024

To investigate the effects of different light qualities on the growth, photosynthesis, transcriptome, and metabolome of mint, three treatments were designed: (1) 7R3B (70% red light and 30% blue light, CK); (2) 7R3B+ far-red light (FR); (3) 7R3B+ ultraviolet light A (UVA). The results showed that supplemental FR significantly promoted the growth and photosynthesis of mint, as evidenced by the increase in plant height, plant width, biomass, effective quantum yield of PSII photochemistry (Fv’/Fm’), maximal quantum yield of PSII (Fv/Fm), and performance index (PI). UVA and CK exhibited minimal differences. Transcriptomic and metabolomic analysis indicated that a total of 788 differentially expressed genes (DEGs) and 2291 differential accumulated metabolites (DAMs) were identified under FR treatment, mainly related to plant hormone signal transduction, phenylpropanoid biosynthesis, and flavonoid biosynthesis. FR also promoted the accumulation of phenylalanine, sinapyl alcohol, methylchavicol, and anethole in the phenylpropanoid biosynthesis pathway, and increased the levels of luteolin and leucocyanidin in the flavonoid biosynthesis pathway, which may perhaps be applied in practical production to promote the natural antibacterial and antioxidant properties of mint. An appropriate increase in FR radiation might alter transcript reprogramming and redirect metabolic flux in mint, subsequently regulating its growth and secondary metabolism. Our study uncovered the regulation of FR and UVA treatments on mint in terms of growth, physiology, transcriptome, and metabolome, providing reference for the cultivation of mint and other horticultural plants.
Effect of Selenium, Copper and Manganese Nanocomposites in Arabinogalactan Matrix on Potato Colonization by Phytopathogens Clavibacter sepedonicus and Pectobacterium carotovorum
Authors: Perfileva Alla I. , Zakharova Olga V. , Graskova Irina A. , Krutovsky Konstantin V.
Published: December 2024

The effect of chemically synthesized nanocomposites (NCs) of selenium (Se/AG NC), copper oxide (Cu/AG NC) and manganese hydroxide (Mn/AG NC), based on the natural polymer arabinogalactan (AG), on the processes of growth, development and colonization of potato plants in vitro was studied upon infection with the causative agent of potato blackleg—the Gram-negative bacterium Pectobacterium carotovorum—and the causative agent of ring rot—the Gram-positive bacterium Clavibacter sepedonicus (Cms). It was shown that the infection of potatoes with P. carotovorum reduced the root formation of plants and the concentration of pigments in leaf tissues. The treatment of plants with Cu/AG NC before infection with P. carotovorum stimulated leaf formation and increased the concentration of pigments in them. A similar effect was observed when potatoes were exposed to Mn/AG NC, and an increase in growth and root formation was also observed. The infection of plants with Cms inhibited plant growth. Treatment with each of the NCs mitigated this negative effect of the phytopathogen. At the same time, Se/AG and Mn/AG NCs promoted leaf formation. The Se/AG NC increased the biomass of Cms-infected plants. The treatment of plants with NCs before infection showed a decrease in the intensity of the colonization of plants by bacteria. The Se/AG NC had the maximum effect, which is probably due to its high antioxidant capacity. Thus, the NCs are able to mitigate the negative effects of bacterial phytopathogens on vegetation and the intensity of colonization by these bacteria during the infection of cultivated plants.
Vibration Welding of PLA/PHBV Blend Composites with Nanocrystalline Cellulose
Authors: Bazan Patrycja , Kozub Barbara , Rochman Arif , Melnychuk Mykola , Majewska Paulina , Mroczka Krzysztof
Published: December 2024

Thermoplastic composites have garnered significant attention in various industries due to their exceptional properties, such as recyclability and ease of molding. In particular, biocomposites, which combine biopolymers with natural fibers, represent a promising alternative to petroleum-based materials, offering biodegradability and reduced environmental impact. However, there is limited knowledge regarding the efficacy of joining PLA/PHBV-based biocomposites modified with nanocrystalline cellulose (NCC) using vibration welding, which restricts their potential applications. This study demonstrates that vibration welding enables efficient bonding of PLA/PHBV composites with NCC, resulting in strong, biodegradable, and environmentally friendly materials. The investigation revealed that the addition of nanocrystalline cellulose (NCC) at 5, 10, and 15 wt.% significantly enhanced the strength of welded joints, with the highest strength achieved at 15% NCC content. Microstructural analysis using scanning electron microscopy (SEM) and deformation studies with digital image correlation (DIC) indicated that a higher NCC content led to greater local deformation, reducing the risk of brittle fracture. Mechanical hysteresis tests confirmed the composites’ favorable resistance to variable loads, highlighting their stability and energy dissipation capabilities. Optimization of welding parameters, such as vibration amplitude, welding time, and pressure, is crucial for achieving optimal mechanical performance. These findings suggest that PLA/PHBV composites modified with NCC can be utilized as durable and eco-friendly materials in various industries, including automotive and packaging. This research presents new opportunities for the development of biodegradable high-strength materials that can serve as alternatives to traditional plastics.
Cumulative Strain and Improvement Mechanisms of Soil Reinforced by Xanthan Gum Biopolymer Under Traffic Loading
Authors: Yang Liu , An Lingshi , Yan Kuangyu , Du Gaofeng
Published: December 2024

As is widely accepted, cumulative strain and improvement mechanisms of stabilized soil are critical factors for the long-term reliable operation of expressways and high-speed railways. Based on relevant research findings, xanthan gum biopolymer is regarded as a green and environmentally friendly curing agent in comparison to traditional stabilizers, such as cement, lime, and fly ash. However, little attention has been devoted to the cumulative strain and improvement mechanisms of soil reinforced by xanthan gum biopolymer under traffic loading. In the current study, a series of laboratory tests, including cyclic triaxial tests and scanning electron microscopy (SEM) tests, were performed to investigate this issue in more detail. The influences of xanthan gum biopolymer content, curing time, moisture content, confining pressure, and cyclic stress amplitude on cumulative strain were analyzed. In addition, the cumulative strain model was proposed to provide a good description of experimental data. Finally, the microscopic structure of soil reinforced by xanthan gum biopolymer was analyzed to discuss the improvement mechanisms. The results show that the cumulative strain is strongly influenced by xanthan gum biopolymer content. For a given number of loading cycles, the greater the confining pressure, the smaller the cumulative strain. The calculated results of the cumulative strain model show a good agreement with test data. The “flocculent” hydrogel can form a denser structure and greater bonding strength in comparison to the “branch-like” and “net-like” hydrogels.