bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Analysis of Alternative Splicing in Moso Bamboo and Its Dwarf Mutant, Phyllostachys edulis ‘Tubaeformis’
Authors: Qiu Zhenhua , Sun Yuanyuan , Su Yanhui , Cheng Long , Liu Dong , Lin Shuyan , Li Long
Published: July 2024

Internode length is a crucial phenotypic trait of bamboo, significantly impacting its processing and utilization. Phyllostachys edulis ‘Tubaeformis’ (Shengyin Bamboo), a variety of Moso bamboo, exhibits drastically shortened internodes, making it a valuable ornamental bamboo species. We used PacBio single-molecule long-read sequencing and second-generation sequencing to identify genome-wide alternative splicing (AS) events in Moso bamboo and its dwarf mutant, Shengyin bamboo, and compared the differences between the two. Our sequencing data unveiled 139,539 AS events, with retained introns as the most prevalent events. A large number of genes were differentially alternatively spliced (DAS) between Moso bamboo and Shengyin bamboo, and genes related to RNA splicing were most significantly enriched. The high expression of SR isoforms in the 24th internode of Moso bamboo is likely the main factor leading to its greater number of alternative splicing events. Alternative splicing affects the functional domains of partial GRF, E2F, and NAM isoforms, leading to the loss of domains in some isoforms and enabling some isoforms to acquire new functional domains, and this phenomenon is more common in Shengyin bamboo. AS modifies the functional domains of certain GRF isoforms, frequently resulting in domain losses or endowing isoforms with novel domains, and this phenomenon is more common in Shengyin bamboo. We used PacBio single-molecule long-read sequencing and second-generation sequencing to identify genome-wide alternative splicing (AS) events in Moso bamboo and its dwarf mutant, Shengyin Bamboo and compared the differences between the two.
Optimization of Biodegradation of Common Bean Biomass for Fermentation Using Trichoderma asperellum WNZ-21 and Artificial Neural Networks
Authors: Alrdahe Salma Saleh , Moussa Zeiad , Alanazi Yasmene F. , Alrdahi Haifa , Saber WesamEldin I. A. , Darwish Doaa Bahaa Eldin
Published: July 2024

This study showcases a promising approach to sustainably unlocking plant biomass residues by combining biodegradation with artificial intelligence to optimize the process. Specifically, we utilized the definitive screening design (DSD) and artificial neural networks (ANNs) to optimize the degradation of common bean biomass by the endophytic fungus Trichoderma asperellum WNZ-21. The optimized process yielded a fungal hydrolysate rich in 12 essential and non-essential amino acids, totaling 18,298.14 μg/g biomass. GC-MS analysis revealed four potential novel components not previously reported in microbial filtrates or plants and seven components exclusive to plant sources but not reported in microbial filtrates. The hydrolysate contained phenolic, flavonoid, and tannin compounds, as confirmed by FT-IR analysis. High-resolution transmission electron microscopy depicted structures resembling amino acid micelles and potential protein aggregates. The hydrolysate exhibited antioxidant, antibacterial, and anticancer properties and innovatively induced apoptotic modulation in the MCF7 cancer cell line. These findings underscore the potential of ANN-optimized fermentation for various applications, particularly in anticancer medicine due to its unique composition and bioactivities. The integration of the DSD and ANNs presents a novel technique for biomass biodegradation, warranting the valorization of plant biomass and suggesting a further exploration of the new components in the fungal hydrolysate. This approach represents the basic concept for exploring other biomass sources and in vivo studies.
Multi-Agent Dynamic Fog Service Placement Approach
Authors: Šatkauskas Nerijus , Venčkauskas Algimantas
Published: July 2024

Fog computing as a paradigm was offered more than a decade ago to solve Cloud Computing issues. Long transmission distances, higher data flow, data loss, latency, and energy consumption lead to providing services at the edge of the network. But, fog devices are known for being mobile and heterogenous. Their resources can be limited, and their availability can be constantly changing. A service placement optimization is needed to meet the QoS requirements. We propose a service placement orchestration, which functions as a multi-agent system. Fog computing services are represented by agents that can both work independently and cooperate. Service placement is being completed by a two-stage optimization method. Our service placement orchestrator is distributed, services are discovered dynamically, resources can be monitored, and communication messages among fog nodes can be signed and encrypted as a solution to the weakness of multi-agent systems due to the lack of monitoring tools and security.
Post-Fire Vegetation (Non-)Recovery across the Edges of a Wildfire: An Unexplored Theme
Authors: Rossetti Ivo , Calderisi Giulia , Cogoni Donatella , Fenu Giuseppe
Published: July 2024

Wildfires have a significant influence on ecosystems globally, shaping vegetation, biodiversity, landscapes, soil properties, and other ecosystem processes. Despite extensive research on different aspects of wildfires, the edges of burned areas remain understudied, even though they involve complex dynamics. In this study, we analyzed the post-fire vegetation recovery across the edges of a large wildfire in a Mediterranean area. The investigations were focused on patches of woodlands that, in a previous study, showed a normalized burn ratio (NBR) decline one year after the fire. Field vegetation surveys were carried out in areas characterized by different NBR recovery rates and in areas outside the burned area as controls. Five hypotheses were tested, identifying delayed tree mortality as a key factor linked to NBR decline, particularly in low-severity fire zones in proximity to the fire edges. Delayed mortality, observed predominantly near the edges, may also affect unburned or less severely burned patches within the main fire perimeter, highlighting the need for ongoing monitoring. As these areas play a crucial role in the post-fire succession and vegetation dynamics, understanding the second-order effects of a fire is imperative for effective ecosystem management. This study underscores the importance of the long-term assessment of fire impacts, emphasizing the necessity of field surveys alongside remote sensing. Continued observation is essential to elucidate the enduring impacts of wildfires and to facilitate informed restoration strategies.
Clinical and Histopathological Evolution of Acute Intraperitoneal Infection by Streptococcus agalactiae Serotypes Ib and III in Nile Tilapia
Authors: Ferrari Natália Amoroso , Favero Leonardo Mantovani , Facimoto Cesar Toshio , Dall Agnol Alais Maria , Gaeta Marcos Letaif , de Oliveira Thalita Evani Silva , Gonçalves Daniela Dib , Lopera-Barrero Nelson Maurício , Pereira Ulisses de Pádua , Di Santis Giovana Wingeter
Published: July 2024

Streptococcus agalactiae is a highly invasive bacterium that causes significant economic losses in tilapia aquaculture around the world. Furthermore, it is a pathogen for mammals, including humans, emphasizing its importance in One Health. The aim of this work was to evaluate the evolution of clinical and histopathological lesions caused by acute infection with two serotypes of S. agalactiae. For this, two strains isolated from natural outbreaks in Brazilian aquaculture farms (S13, serotype Ib; S73, serotype III) were used to challenge juvenile Nile tilapia (Oreochromis niloticus) intraperitoneally. Target organ samples were collected ten times, between 1 and 96 h post-infection, for microbiological and histopathological analyses. Anorexia was the first clinical sign and the first death occurred at 24 and 30 h in the fish infected with strains S13 and S73, respectively. Serotype Ib initially caused more pronounced lesions in the nervous system; however, serotype III lesions progressed more aggressively, reaching the same severity as those of serotype Ib. This trend was repeated in the mortality curve after 32 h. These results elucidated the important stages in the pathogenesis of S. agalactiae serotypes Ib and III in tilapia and suggest “tips and tricks” to improve the positive culture rate in the clinical diagnosis of infections in some tissues.
Community Structure Characteristics and Changes in Fish Species at Poyang Lake after the Yangtze River Fishing Ban
Authors: Zhang Yanping , Zhang Haixin , Wu Zijun , Zhao Mingguang , Feng Guangpeng
Published: July 2024

To understand the fish community structure characteristics and changes in Poyang Lake after the Yangtze River fishing ban in 2021, seven sampling stations were established, namely in Hukou, Lushan, Duchang, Yongxiu, Nanjishan, Ruihong, and Poyang, for fishery resource surveys between 2020 and 2021. The results showed that 93 fish species were identified, belonging to 11 orders, 20 families, and 61 genera, which was an increase of 4.5% from before the fishing ban. Of these, 52 Cyprinidae species were identified, comprising the highest proportion (55.91%). Four invasive alien species (hybrid sturgeon, Cirrhina mrigala, Piaractus brachypomus, and Mugil cephalus) were identified, and the number of alien species in Poyang Lake was higher than before the fishing ban. Carnivorous and mid-lower-level fish showed a significant increase, accounting for 47.31% and 38.71% of the total species, respectively. Compared with the Yangtze River before the fishing ban, the body length and body weight of the main economic fish in Poyang Lake increased by 6.10–61.26% and by 15.14–291.57%, respectively. In terms of age structure, the proportion of major economically important fish aged 1 or 2 years decreased significantly, while the proportion of older fish increased substantially. There was little difference in the diversity of fish communities at different stations in Poyang Lake. In terms of biodiversity, the Shannon–Wiener index ranged from 2.158 to 2.909, with Poyang having the highest value and Nanjishan the lowest. Margalef’s index ranged from 4.265 to 6.459, with Lushan having the highest value and Nanjishan the lowest. Pielou’s index ranged from 0.617 to 0.822, with Duchang having the highest value and Nanjishan the lowest. Hence, the Yangtze river fishing ban has played an important and positive role in the restoration of fishery resources in Poyang Lake. However, long-term tracking and monitoring are needed to provide information to comprehensively evaluate the ecological impact of the Poyang Lake fishing ban.
Numerical Study on the Impact Pressure of Droplets on Wind Turbine Blades Using a Whirling Arm Rain Erosion Tester
Authors: Fujisawa Nobuyuki , Kawabata Hirokazu
Published: July 2024

The leading-edge erosion of a wind turbine blade was tested using a whirling arm rain erosion tester, whose rotation rate is considerably higher than that of a full-scale wind turbine owing to the scale effect. In this study, we assessed the impact pressure of droplets on a wet surface of wind turbine blades using numerical simulation of liquid droplet impact by solving the Navier–Stokes equations combined with the volume-of-fluid method. This was conducted in combination with an estimation of liquid film thickness on the rotating blade using an approximate solution of Navier–Stokes equations considering the centrifugal and Coriolis forces. Our study revealed that the impact pressure on the rain erosion tester exceeded that on the wind turbine blade, attributed to the thinner liquid film on the rain erosion tester than on the wind turbine blade caused by the influence of centrifugal and Coriolis forces. This indicates the importance of correcting the influence of liquid-film thickness in estimating the impact velocity of droplets on the wind turbine blade. Furthermore, we demonstrated the correction procedure when estimating the impact velocity of droplets on the wind turbine blade.
A Comparison Analysis of Quality and Metabolic Compounds in Lilies with Different Drying Treatments
Authors: Xie Lixia , Liu Jiajia , Wu Haoyu , Zhong Yueyan , Liu Xueying , Li Guangli , Liu Zhi
Published: July 2024

The present study aimed to investigate the variations in the nutritional composition, antioxidant capacity, and metabolite profile of lilies subjected to different drying treatments, including vacuum freeze drying (VFD), hot air drying (HAD), vacuum drying (VD), and infrared drying (ID). The results show that VFD provided better preservation of the original coloration and displayed the highest levels of total amino acid content, total phenolic content, total flavonoid content, and polysaccharide and alkaloid content. Our results reveal that VFD treatment can be employed to obtain high-quality lilies with desirable appearance characteristics and nutrient compositions. Metabolomics analysis identified a total of 464 metabolites from various dried lilies. Differential metabolite screening found 150 differential metabolites across all pairwise comparisons. Hierarchical clustering analysis (HCA) indicated that lilies subjected to VFD treatment exhibited a higher abundance of steroids, saponin, flavonoids, and phenolic glycoside, whereas those subjected to HAD, VD, or ID treatments showed relatively elevated levels of specific amino acids or derivatives. This study elucidates the significant impact of various drying treatments on the quality and metabolic profile of lilies, thereby providing valuable insights for enhancing the nutritional quality of processed lilies.
Berry Fruits and Their Improving Potential on Skeletal Muscle Health and Performance: A Systematic Review of the Evidence in Animal and in Human Studies
Authors: Moroni Alessia , Zupo Roberta , Castellana Fabio , Amirante Federica , Zese Marco , Rondanelli Mariangela , Riso Patrizia , Perna Simone
Published: July 2024

The well-established anti-inflammatory and antioxidant properties of red fruits leave room for a biological pathway of improved muscle health promoted by berries in the diet. Our objective was to systematically review the number of trials conducted on human and animal species around the relationship between a berry diet and muscle health outcomes. Two independent examiners conducted a search for studies that utilized keywords associated with muscle health outcomes and a berry-based diet in both human and animal trials, in accordance with the PRISMA statement guidelines. The literature was searched through six electronic databases until December 2023. Screening of 152 retrieved articles resulted in a final selection of 16 reports investigating the effect of exposure to a berry-based diet and skeletal muscle health outcomes. The study protocol was registered on PROSPERO (CRD42023479682). Among the selected studies, nine involved humans and seven animal models (rats and mice). Overall, most of the studies reported positive effects on performance or muscle health. Specifically, five studies investigated the possible effects of blackcurrant on active human subjects or athletes; three studies focused on blueberry and presented results on running performance (human sample) and muscle health (rat models). The rest of the studies involved raspberries (two studies, rat models), aronia (one study, rat models), elderberry (one study, rat models), and a mixed compound (one study, rat models). In conclusion, there is some early evidence that a berry-rich diet may increase performance or muscle health, but more research is needed to fully understand the underlying biological trajectories, and thus, no firm conclusions can yet be drawn.
Chemical Characterization and Biological Activities Evaluation of Myrtus communis L. Essential Oil Extraction By-Product towards Circular Economy and Sustainability
Authors: Abdessemed Meriem , Bouacida Saoussen , Turki Mohamed , Ben Haj Koubaier Hayet , Omrani Souha , Allouache Radia , Bouzouita Nabiha , Karoui Romdhane , Snoussi Ahmed
Published: July 2024

Essential oil (EO) extraction is a widespread practice generating huge amounts of solid plant by-products a potential source of bioactive compounds, on the one hand, and a detrimental risk for the environment that needs to be carefully considered on the other hand. The present study aims to valorize Myrtus communis L. leaf by-products obtained following EO extraction using a steam distillation unit through the recovery of phenolic compounds and the evaluation of their biological activities. The total phenols, flavonoids, and proanthocyanidins contents of the ethanolic extract by-product were higher than the control (leaves without extraction of EO). Their amounts increased from 69.30 to 88.06 mg GAE/g for total phenols, from 36.31 to 70.97 mg QE for flavonoids and from 19.74 to 21.49 mg CE/g of extract for proanthocyanidins. The identification of phenolic compounds by high-performance liquid-chromatography equipped with a reversed-phase (RP-HPLC) system revealed that the by-product sample includes more gallic acid, catechin, syringic acid and luteolin 7-O-glucoside but less p-coumaric acid and kaempferol than the control. Moreover, the mid-infrared spectroscopy (MIR) showed the presence of benzene ring characteristic of phenolic compounds at 756 cm−1, esters of aromatic acids and stretching vibrations of polyphenols at 1141–1234 cm−1, C=C stretching present in phenolic acids such as coumaric acid and catechin at 1604 cm−1. The assessment of antioxidant activity revealed that the ABTS+• radical scavenging activity was significantly increased, whereas the DPPH• radical inhibition activity and the ferric reduction antioxidant power were significantly decreased. The results indicated, as well, that Myrtus communis L. leaf by-products maintained a considerable antibacterial activity depending on the tested bacterial strain. Additionally, the anti-α-amylase activity was higher for the Myrtus communis L. leaf by-product extract. Therefore, Myrtus communis L. leaf by-products of EO extraction offer phenolic compounds with significant biological activities, contributing to the sustainable development and the promotion of circular economy by the recovery of valuable inputs from plant by-products.
Tracking Trace Elements Found in Coffee and Infusions of Commercially Available Coffee Products Marketed in Poland
Authors: Pokorska-Niewiada Kamila , Scheffler Aniela , Przedpełska Laura , Witczak Agata
Published: July 2024

Coffee is a source of micronutrients, including iron, zinc, copper, and manganese. It may also contain toxic metals, such as lead and cadmium. The effects of coffee on the human body may vary depending on its composition. The objective of this study was to assess the quality of ground and instant coffee with regard to the content of selected trace elements. The concentrations of trace elements, including copper, iron, manganese, and zinc, were determined by ICP-AES, while the levels of lead and cadmium were quantified by GF-AAS methods. Furthermore, the degree of coverage of the recommended intake of elements and the risk assessment for human health (EDI, THQ, PTMI, and TWI) were determined. Our findings indicate that the consumption of a cup of coffee provides the body with only small amounts of these elements. A coffee prepared from 6.33 g of ground coffee beans provides 0.08–1.52% of the RDA value, while a coffee prepared from 6.33 g of instant coffee provides 0.46–13.01% of the RDA, depending on the microelement. The low transfer to the brew (Pb = 7.1%; Cd = 30.0%) of the analyzed ground coffees renders them safe for the consumer, even at a consumption of six cups per day. The percentage of benchmark dose lower confidence limit (BMDL0.1) in the case of lead did not exceed 0.9%. The estimated value did not exceed 0.2% of the provisional tolerable monthly intake of cadmium (PTMI). None of the analyzed coffees exhibited any risk regarding the trace elements.
A Comprehensive Review on Harnessing Soy Proteins in the Manufacture of Healthy Foods through Extrusion
Authors: Huang Yuyang , Liu Linlin , Sun Bingyu , Zhu Ying , Lv Mingshou , Li Yang , Zhu Xiuqing
Published: July 2024

The global development of livestock production systems, accelerated by the growing demand for animal products, has greatly contributed to land-use change, greenhouse gas emissions, and pollution of the local environment. Further, excessive consumption of animal products has been linked with cardiovascular diseases, digestive system diseases, diabetes, and cancer. On the other hand, snacks, pasta, and bread available on the market are made from wheat, fat, salt, and sugar, which contribute to the risk of cardiovascular diseases. To counter these issues, a range of plant protein-based food products have been developed using different processing techniques, such as extrusion. Given the easy scalability, low cost of extrusion technology, and health benefits of soy proteins, this review focuses on the extrusion of soy protein and the potential application of soy protein-based extrudates in the manufacture of healthy, nutritious, and sustainable meat analogs, snacks, pasta products, and breakfast cereals. This review discusses the addition of soy protein to reformulate hypercaloric foods through extrusion technology. It also explores physical and chemical changes of soy proteins/soy protein blends during low and high moisture extrusion. Hydrogen bonds, disulfide bonds, and hydrophobic interactions influence the properties of the extrudates. Adding soy protein to snacks, pasta, breakfast cereals, and meat analogs affects their nutritional value, physicochemical properties, and sensory characteristics. The use of soy proteins in the production of low-calorie food could be an excellent opportunity for the future development of the soybean processing industry.
Step-by-Step Metagenomics for Food Microbiome Analysis: A Detailed Review
Authors: Sadurski Jan , Polak-Berecka Magdalena , Staniszewski Adam , Waśko Adam
Published: July 2024

This review article offers a comprehensive overview of the current understanding of using metagenomic tools in food microbiome research. It covers the scientific foundation and practical application of genetic analysis techniques for microbial material from food, including bioinformatic analysis and data interpretation. The method discussed in the article for analyzing microorganisms in food without traditional culture methods is known as food metagenomics. This approach, along with other omics technologies such as nutrigenomics, proteomics, metabolomics, and transcriptomics, collectively forms the field of foodomics. Food metagenomics allows swift and thorough examination of bacteria and potential metabolic pathways by utilizing foodomic databases. Despite its established scientific basis and available bioinformatics resources, the research approach of food metagenomics outlined in the article is not yet widely implemented in industry. The authors believe that the integration of next-generation sequencing (NGS) with rapidly advancing digital technologies such as artificial intelligence (AI), the Internet of Things (IoT), and big data will facilitate the widespread adoption of this research strategy in microbial analysis for the food industry. This adoption is expected to enhance food safety and product quality in the near future.
From Waste to Consumption: Tomato Peel Flour in Hamburger Patty Production
Authors: Karslıoğlu Betül , Soncu Eda Demirok , Nekoyu Beyzanur , Karakuş Erdem , Bekdemir Gülsedef , Şahin Barış
Published: July 2024

Tomato is a widely cultivated crop and its processing produces large quantities of wastes, such as pulp, seed, and peel. In recent years, the valorization of these wastes in the production of high-value-added food products has gained popularity in achieving environmental sustainability and zero waste. From this viewpoint, dried tomato peel (DTP-1%, 2%, 3%, 4%) flour was included in hamburger formulations. In patty samples, ash, carbohydrate, and dietary fiber amounts were increased due to the high fiber content of DTP flour, while moisture and fat percentages decreased with increasing amounts of DTP flour (p < 0.05). The inclusion of DTP flour retarded lipid oxidation during cooking (p < 0.05). The significantly highest cooking yield was calculated in samples including 4% DTP flour. In parallel, water-holding capacity, moisture, and fat retention values increased with increasing levels of DTP flour (p < 0.05). The enrichment of patties with DTP flour resulted in hard texture, less gumminess, and a darker, more reddish and yellowish color (p < 0.05). Hamburger samples containing 1% or 2% DTP flour were graded with closer scores in the sensory panel as compared to the control (0% DTP). Overall, our findings demonstrated that DTP flour up to 2% could be used to improve the nutritional and technological properties of patty samples.
Flavoromics Analysis of Passion Fruit-Roasted Chicken
Authors: Mao Ya , Liu Qi , Shao Jianwei , Yang Li , Zhang Xuewu
Published: July 2024

Currently, research on the flavor components and their dynamic changes in roasted chicken with a special flavor is rare. In this study, a passion fruit-roasted chicken was prepared, its characteristic flavor components were profiled by flavoromics, and their evolution patterns and precursors were determined. The results showed that the characteristic flavor component with the highest contribution rate was ethyl butyrate (50.44%). In particular, some unique flavor compounds were identified compared with other roasted chicken products available. The main volatile flavor components in all stages of processing were alcohols, esters, and hydrocarbons, 15 to 30 min of roasting is an important stage for establishing the aroma system, and at the end, hydrocarbons were the main volatile compounds. During the 30-day storage period, the characteristic flavor components included ethyl butyrate, ethyl maltol, β-caryophyllene, and guaiacene. In conclusion, passion fruit-roasted chicken contained many characteristic flavor components, which were mainly formed within 15 to 30 min of roasting and were basically stable during the 30-day storage period. In a word, this work prepared a novel roasted chicken and revealed its mechanism of flavor formation at different baking stages and storage periods, which provided references for industrial production.
Application of Plant Antimicrobials in the Food Sector: Where Do We Stand?
Authors: Pinto Loris , Ayala-Zavala Jesús Fernando
Published: July 2024

The Special Issue “Plant Extracts Used to Control Microbial Growth: Efficacy, Stability and Safety Issues for Food Applications” explored the potential of plant-based extracts as natural antimicrobial agents in the food industry. Its purpose was to address the growing demand for natural, safe, and effective food preservation methods. The contributions highlighted various plant extracts’ antimicrobial efficacy, including phenolic compounds, terpenes, and other bioactive substances. Research papers and one review were submitted from countries, including Spain, Portugal, Italy, Mexico, Turkey, India, USA, Romania, China, and Lithuania, showcasing a diverse international collaboration. Key topics covered in this issue included the chemical characterization of plant extracts, their stability under different processing and storage conditions, and their safety assessments. Advances were reported in using plant extracts to inhibit spoilage microorganisms and foodborne pathogens, enhance food safety, and extend shelf life. The published papers in the Special Issue studied various food types, including yogurt, catfish fillets, edible Mushrooms, red grapes, herring Fillets, and various food types covered in the review. This diversity demonstrates the broad applicability of plant extracts across different food products. Notable findings included the antimicrobial activities of fermented grapevine leaves, grapefruit seed extract, cinnamaldehyde, clove oil, and other plant-based compounds. In conclusion, this Special Issue demonstrated significant progress in applying plant extracts for food preservation, highlighting their potential to contribute to safer and more sustainable food systems worldwide.
Inactivation of Bacillus cereus Spores and Vegetative Cells in Inert Matrix and Rice Grains Using Low-Pressure Cold Plasma
Authors: Valdez-Narváez María Inés , Fernández-Felipe M. Teresa , Martinez Antonio , Rodrigo Dolores
Published: July 2024

This study investigated the effects of low-pressure cold plasma on the inactivation of Bacillus cereus vegetative cells and spores in an inert matrix (borosilicate glass slide) and in rice grains, using oxygen as ionization gas. Greater reductions in B. cereus counts were observed in vegetative cells rather than spores. The experimental data obtained show that both the power of the plasma treatment and the matrix proved to be determining factors in the inactivation of both the spores and vegetative cells of B. cereus. To characterize the inactivation of B. cereus, experimental data were accurately fitted to the Weibull model. A significant decrease in parameter “a”, representing resistance to treatment, was confirmed with treatment intensification. Furthermore, significant differences in the “a” value were observed between spores in inert and food matrices, suggesting the additional protective role of the food matrix for B. cereus spores. These results demonstrate the importance of considering matrix effects in plasma treatment to ensure the effective inactivation of pathogenic microorganisms, particularly in foods with low water activity, such as rice. This approach contributes to mitigating the impact of foodborne illnesses caused by pathogenic microorganisms.
Effects of Incorporating Caramel, Carrot, and Tomato Powder on the Quality Characteristics of Soy Protein-Based Meat Patties
Authors: Shi Xinyu , Wang Zun , Fang Zhongxiang
Published: July 2024

Plant protein-based foods have become dietary preferences worldwide, and the quality of this food group is highly associated with the ingredients used. This study investigated the effects of incorporating caramel, tomato powder, and carrot powder on the product quality of soy protein-based meat patties (SPMPs). The color, total phenolic content (TPC), antioxidant activity, lipid oxidation, and texture profile of uncooked and cooked soy protein meat patties were analyzed. Among the cooked SPMPs, caramel SPMPs exhibited the lowest color difference (ΔE) values, and the ΔE value of tomato SPMPs was lower than that of carrot SPMPs, indicating that caramel has the best color stability, and the tomato experienced less color change than the carrot during cooking. Notably, carrot SPMPs exhibited lower color stability during refrigeration storage than the others. Both carrot and tomato powders increased the total phenolic content (TPC) and antioxidant stability and inhibited lipid oxidation in SPMPs during cooking. However, tomato SPMPs exhibited higher TPC values and greater antioxidant stability compared to carrot SPMPs. The addition of caramel and carrot powders decreased the hardness of raw SPMPs, but tomato powder increased the hardness. The texture profile of tomato SPMPs was more affected by the cooking process compared to caramel and carrot SPMPs. This study showed that incorporating both carrot and tomato powders positively influenced the quality characteristics of SPMPs compared to caramel powder, however, tomato powder exhibited superior efficacy.
Ballistic Gels in Experimental Fracture Setting
Authors: Biehl Christoph , Thiesse-Kraul Ann-Cathrin , Stötzel Sabine , Alzubi Salsabel , Biehl Lotta , Mülke Matthias , Heiss Christian , El Khassawna Thaqif
Published: July 2024

Biomechanical tests typically involve bending, compression, or shear stress, while fall tests are less common. The main challenge in performing fall tests is the non-reproducible directionality of bone when tested with soft tissue. Upon removal of the soft tissue, the explanted bone’s resistance to impact diminishes. Therefore, ballistic gels can fix specimens in reproducible directions and simulate periosteal soft tissue. However, the use of ballistic gels in biomechanical studies is neither standardized nor widespread. This study aimed to optimize a ballistic gel consistency that mimics the upper thigh muscle in sheep. Our results suggest a standardized and flexible evaluation method by embedding samples in ballistic gel. Compression tests were conducted using cylindrical pieces of gluteal muscle from sheep. Various compositions of agarose and gelatin mixtures were tested to achieve a muscle-like consistency. The muscle-equivalent ballistic gel was found to consist of 29.5% gelatin and 0.35% agarose. Bones remained stable within the ballistic gel setup after freeze–thaw cycles between −20 °C and +20 °C. This method reduces the variability caused by muscle and improves storage quality, allowing for tests to be conducted under consistent conditionsBallistic gels of agarose and gelatin are suitable for bone fracture models. They have muscle-like strength, fix fractures simultaneously, are inexpensive to produce, and can be stored to allow repeated measurements of the same object with changing questions.
High-Performance Supercapacitor Electrodes from Fully Biomass-Based Polybenzoxazine Aerogels with Porous Carbon Structure
Authors: Periyasamy Thirukumaran , Asrafali Shakila Parveen , Lee Jaewoong
Published: July 2024

In recent years, polybenzoxazine aerogels have emerged as promising materials for various applications. However, their full potential has been hindered by the prevalent use of hazardous solvents during the preparation process, which poses significant environmental and safety concerns. In light of this, there is a pressing need to explore alternative methods that can mitigate these issues and propel the practical utilization of polybenzoxazine aerogels. To address this challenge, a novel approach involving the synthesis of heteroatom self-doped mesoporous carbon from polybenzoxazine has been devised. This process utilizes eugenol, stearyl amine, and formaldehyde to create the polybenzoxazine precursor, which is subsequently treated with ethanol as a safer solvent. Notably, the incorporation of boric acid in this method serves a dual purpose: it not only facilitates microstructural regulation but also reinforces the backbone strength of the material through the formation of intermolecular bridged structures between polybenzoxazine chains. Moreover, this approach allows ambient pressure drying, further enhancing its practicability and environmental friendliness. The resultant carbon materials, designated as ESC-N and ESC-G, exhibit distinct characteristics. ESC-N, derived from calcination, possesses a surface area of 289 m2 g−1, while ESC-G, derived from the aerogel, boasts a significantly higher surface area of 673 m2 g−1. Furthermore, ESC-G features a pore size distribution ranging from 5 to 25 nm, rendering it well suited for electrochemical applications such as supercapacitors. In terms of electrochemical performance, ESC-G demonstrates exceptional potential. With a specific capacitance of 151 F g−1 at a current density of 0.5 A g−1, it exhibits superior energy storage capabilities compared with ESC-N. Additionally, ESC-G displayed a more pronounced rectangular shape in its cyclic voltammogram at a low voltage scanning rate of 20 mV s−1, indicative of enhanced electrochemical reversibility. The impedance spectra of both carbon types corroborated these findings, further validating the superior performance of ESC-G. Furthermore, ESC-G exhibits excellent cycling stability, retaining its electrochemical properties even after 5000 continuous charge–discharge cycles. This robustness underscores its suitability for long-term applications in supercapacitors, reaffirming the viability of heteroatom-doped polybenzoxazine aerogels as a sustainable alternative to traditional carbon materials.
Sulfotransferase 4A1 Coding Sequence and Protein Structure Are Highly Conserved in Vertebrates
Authors: van Waardenburg Robert , Falany Charles
Published: July 2024

Cytosolic sulfotransferases (SULTs) are Phase 2 drug-metabolizing enzymes that catalyze the conjugation of sulfonate to endogenous and xenobiotic compounds, increasing their hydrophilicity and excretion from cells. To date, 13 human SULTs have been identified and classified into five families. SULT4A1 mRNA encodes two variants: (1) the wild type, encoding a 284 amino acid, ~33 kDa protein, and (2) an alternative spliced variant resulting from a 126 bp insert between exon 6 and 7, which introduces a premature stop codon that enhances nonsense-mediated decay. SULT4A1 is classified as an SULT based on sequence and structural similarities, including PAPS-domains, active-site His, and the dimerization domain; however, the catalytic pocket lid ‘Loop 3’ size is not conserved. SULT4A1 is uniquely expressed in the brain and localized in the cytosol and mitochondria. SULT4A1 is highly conserved, with rare intronic polymorphisms that have no outward manifestations. However, the SULT4A1 haplotype is correlated with Phelan–McDermid syndrome and schizophrenia. SULT4A1 knockdown revealed potential SULT4A1 functions in photoreceptor signaling and knockout mice display hampered neuronal development and behavior. Mouse and yeast models revealed that SULT4A1 protects the mitochondria from endogenously and exogenously induced oxidative stress and stimulates cell division, promoting dendritic spines’ formation and synaptic transmission. To date, no physiological enzymatic activity has been associated with SULT4A1.
Genetic Ancestry and Self-Reported “Skin Color/Race” in the Urban Admixed Population of São Paulo City, Brazil
Authors: Pereira Jaqueline , de Souza Camila , Neyra Jennyfer , Leite Jean , Cerqueira Andressa , Mingroni-Netto Regina , Soler Julia , Rogero Marcelo , Sarti Flavia , Fisberg Regina
Published: July 2024

Epidemiological studies frequently classify groups based on phenotypes like self-reported skin color/race, which inaccurately represent genetic ancestry and may lead to misclassification, particularly among individuals of multiracial backgrounds. This study aimed to characterize both global and local genome-wide genetic ancestries and to assess their relationship with self-reported skin color/race in an admixed population of Sao Paulo city. We analyzed 226,346 single-nucleotide polymorphisms from 841 individuals participating in the population-based ISA-Nutrition study. Our findings confirmed the admixed nature of the population, demonstrating substantial European, significant Sub-Saharan African, and minor Native American ancestries, irrespective of skin color. A correlation was observed between global genetic ancestry and self-reported color-race, which was more evident in the extreme proportions of African and European ancestries. Individuals with higher African ancestry tended to identify as Black, those with higher European ancestry tended to identify as White, and individuals with higher Native American ancestry were more likely to self-identify as Mixed, a group with diverse ancestral compositions. However, at the individual level, this correlation was notably weak, and no deviations were observed for specific regions throughout the individual’s genome. Our findings emphasize the significance of accurately defining and thoroughly analyzing race and ancestry, especially within admixed populations.
Grain Size Distribution and Provenance of Holocene Sand from the Sava River (Zagreb, Croatia)
Authors: Barudžija Uroš , Blatančić Matteo , Malvić Tomislav
Published: July 2024

This study involves an investigation into the grain size distribution and provenance of the sand deposited near Zagreb (Croatia) in the riverbed of the regionally important, almost 1000 km long Sava River, which connects several SE European countries. Recent research in the study area has mainly focused on the deposits forming the Zagreb alluvial aquifer system, rather on the Sava River sediment deposited in its riverbed, which is the focus of this study. The grain size distribution results obtained by dry sieving and laser granulometry showed a predominately fine and medium sand deposition at riverbanks and sand point bars. Medium sand increased downstream towards the east, within the artificially more channelized riverbed in the urban area. Fine sand prevailed 50 km further downstream in a more meandering low-relief area, near the city of Sisak and Lonjsko Polje Nature Park. Provenance analysis showed predominately carbonate sand in the western part of the city of Zagreb, originating from distant (Alpine) and local (Medvednica Mt. and Samobor Hills) sources. More siliciclastic sand was deposited in the Sava riverbed in the middle and eastern parts of Zagreb, originating mainly from the Medvednica Mt. The prevailing siliciclastic sand further downstream of the Sava River is probably sourced from the Kupa River tributary. Although various studies of the Zagreb alluvial aquifer system have been conducted so far, this study represents a novelty in its investigation into the grain size distribution of the Sava riverbed sand itself, setting the foundations for investigations in the future.
“The Horror of It Made Me Mad”: Hysterical Narration in Richard Marsh’s The Beetle (1897)
Authors: Fried Ariel
Published: July 2024

This article analyzes the hysterical narration styles of two major characters in Richard Marsh’s The Beetle (1897) to reveal the ways late-Victorian discourses attempted (and often failed) to distance particular social anxieties from their modern origins. Attending to previous literary criticism regarding socially Othered groups of this period—racialized foreigners, New Women, and the urban poor—as well as (pseudo)scientific studies from the 1870s–80s, this reading notes the ways that Victorian cultural biases surrounding race, gender, and class could be projected onto Gothicized, Orientalized figures in literary texts. Pairing a postcolonial examination of the novel’s spatial and temporal elements with a psychoanalytic reading of this text, I argue that the slowing pace in Robert Holt’s narrative and the compulsive repetition of Marjorie Lindon’s both reflect the novel’s disruption of space and time and structurally parallel the symptoms of a “hallucinatory hysterical attack,” as conceived by Josef Breuer and Sigmund Freud. Together, these hysterical narratives reveal the failure of particular cultural and scientific discourses to completely bury Victorian anxieties about modernity into different, explicitly Othered spaces and times by collapsing both space and time in the narration of psychic trauma.
An Assessment of Health Behaviours in Primary Care Patients: A Cross-Sectional Study
Authors: Gawłowska Barbara , Chawłowska Ewelina
Published: July 2024

A healthy lifestyle is a key determinant of health, especially for people with chronic diseases such as diabetes or hypertension. The health behaviours which contribute to a healthy lifestyle include the following: regular physical activity, preventive examinations, maintaining a proper diet and avoiding the consumption of alcohol or cigarettes. They have a significant impact on the disease process, controlling symptoms and preventing complications. The aim of this study was to examine the health behaviours related to diet, physical activity and prevention among Polish primary health care patients and to identify predictors of health behaviours. For this reason, the standardized Health Behaviour Scale (HBS) questionnaire was used. The study was conducted among 269 patients (including 61.71% women) in primary health care facilities. The mean age of participants was 52.89 years (±17.76). The mean HBS score was 36.93 ± 9.66. A statistically significant association was found between HBS scores and such sociodemographic variables as education (p = 0.0061, r = 0.17), body mass index (p = 0.0018, r = −0.20, β = −0.36) and self-assessed economic status (p = 0.0094, r = 0.16). Women’s health behaviours as measured by HBS were significantly better than men’s (p < 0.001, β = −6.82). A special focus should be given to the groups manifesting poorer health behaviours (e.g., men, older people and persons with low socioeconomic status) by offering them tailored health-promoting interventions.
Lower Urinary Tract Symptoms Correlate with Erectile Dysfunction and Premature Ejaculation but Not with Men’s Sexual Activity: Results from a Large Population-Representative Study
Authors: Przydacz Mikolaj , Rajwa Pawel , De Cillis Sabrina , Guillot-Tantay Cyrille , Herve Francois , Tienza Fernandez Antonio , Tutolo Manuela , Culha Mehmet Gokhan , Geretto Paolo , Shenhar Chen , Miszczyk Marcin , Chlosta Piotr , Phe Veronique , Osman Nadir
Published: July 2024

Background: Lower urinary tract symptoms (LUTS) contribute to erectile dysfunction (ED) and premature ejaculation (PE). However, only a few studies have been conducted with representative groups of men that had well-balanced demographic characteristics. Thus, we aimed to confirm the effect of LUTS on ED and PE and to analyze the association between LUTS and men’s sexual activity in a large representative cohort. In addition, we evaluated the sex-specific and overall quality of life of men who had LUTS with either ED or PE. Methods: We used the latest census and estimated the sample size to build a group of men representative of the population. LUTS, ED, and PE were evaluated with reliable instruments. Regression models were used to analyze the data. Results: All included men were representative in relation to their age and residential location (n = 3001). ED and PE were more common in men who reported LUTS compared with men who did not have LUTS (p < 0.001). Age, comorbidity, and lifestyle did not affect the negative effect of LUTS on ED or PE (regression coefficients of 0.159 and 0.528 for ED and PE, respectively, p < 0.001). However, regression models did not validate the impact of LUTS on sexual activity, defined by intercourse frequency and number of sexual partners (odds ratio of 0.981, CI 0.961–1.001, p = 0.061). Nevertheless, men with LUTS and either ED or PE had worse quality of sexual life and general quality of life compared with the remaining respondents. Conclusion: LUTS worsened ED and PE but had no impact on men’s sexual activity. Our findings confirm the recommendations to assess for LUTS in men reporting ED or PE. Clinical Trial Registration: The study was registered with ClinicalTrials.gov (NCT05462171).