bluuebunny/crossref_metadata_2025 huggingface dataset:
Glycerol as Alternative Co-Solvent for Water Extraction of Polyphenols from Carménère Pomace: Hot Pressurized Liquid Extraction and Computational Chemistry Calculations
Authors: Huamán-Castilla Nils Leander , Mariotti-Celis María Salomé , Martínez-Cifuentes Maximiliano , Pérez-Correa José Ricardo
Published: March 2020

Glycerol is a co-solvent for water extraction that has been shown to be highly effective for obtaining polyphenol extracts under atmospheric conditions. However, its efficacy under subcritical conditions has not yet been studied. We assessed different water-glycerol mixtures (15%, 32.5%, and 50%) in a hot pressurized liquid extraction system (HPLE: 10 MPa) at 90 °C, 120 °C, and 150 °C to obtain extracts of low molecular weight polyphenols from Carménère grape pomace. Under the same extraction conditions, glycerol as a co-solvent achieved significantly higher yields in polyphenols than ethanol. Optimal extraction conditions were 150 °C, with 32.5% glycerol for flavonols and 50% for flavanols, stilbenes, and phenolic acids. Considering gallic acid as a model molecule, computational chemistry calculations were applied to explain some unusual extraction outcomes. Furthermore, glycerol, methanol, ethanol, and ethylene glycol were studied to establish an incipient structure–property relationship. The high extraction yields of gallic acid obtained with water and glycerol solvent mixtures can be explained not only by the additional hydrogen bonds between glycerol and gallic acid as compared with the other alcohols, but also because the third hydroxyl group allows the formation of a three-centered hydrogen bond, which intensifies the strongest glycerol and gallic acid hydrogen bond. The above occurs both in neutral and deprotonated gallic acid. Consequently, glycerol confers to the extraction solvent a higher solvation energy of polyphenols than ethanol.
Systematic Review of Level 1 and Level 2 Screening Tools for Autism Spectrum Disorders in Toddlers
Authors: Petrocchi Serena , Levante Annalisa , Lecciso Flavia
Published: March 2020

The present study provides a systematic review of level 1 and level 2 screening tools for the early detection of autism under 24 months of age and an evaluation of the psychometric and measurement properties of their studies. Methods: Seven databases (e.g., Scopus, EBSCOhost Research Database) were screened and experts in the autism spectrum disorders (ASD) field were questioned; Preferred Reporting Items for Systematic review and Meta-Analysis (PRISMA) guidelines and Consensus-based Standard for the selection of health Measurement INstruments (COSMIN) checklist were applied. Results: the study included 52 papers and 16 measures; most of them were questionnaires, and the Modified-CHecklist for Autism in Toddler (M-CHAT) was the most extensively tested. The measures’ strengths (analytical evaluation of methodological quality according to COSMIN) and limitations (in term of Negative Predictive Value, Positive Predictive Value, sensitivity, and specificity) were described; the quality of the studies, assessed with the application of the COSMIN checklist, highlighted the necessity of further validation studies for all the measures. According to COSMIN results, the M-CHAT, First Years Inventory (FYI), and Quantitative-CHecklist for Autism in Toddler (Q-CHAT) seem to be promising measures that may be applied systematically by health professionals in the future.
Total mRNA Quantification in Single Cells: Sarcoma Cell Heterogeneity
Authors: Jonasson Emma , Andersson Lisa , Dolatabadi Soheila , Ghannoum Salim , Åman Pierre , Ståhlberg Anders
Published: March 2020

Single-cell analysis enables detailed molecular characterization of cells in relation to cell type, genotype, cell state, temporal variations, and microenvironment. These studies often include the analysis of individual genes and networks of genes. The total amount of RNA also varies between cells due to important factors, such as cell type, cell size, and cell cycle state. However, there is a lack of simple and sensitive methods to quantify the total amount of RNA, especially mRNA. Here, we developed a method to quantify total mRNA levels in single cells based on global reverse transcription followed by quantitative PCR. Standard curve analyses of diluted RNA and sorted cells showed a wide dynamic range, high reproducibility, and excellent sensitivity. Single-cell analysis of three sarcoma cell lines and human fibroblasts revealed cell type variations, a lognormal distribution of total mRNA levels, and up to an eight-fold difference in total mRNA levels among the cells. The approach can easily be combined with targeted or global gene expression profiling, providing new means to study cell heterogeneity at an individual gene level and at a global level. This method can be used to investigate the biological importance of variations in the total amount of mRNA in healthy as well as pathological conditions.
Parallels of Resistance between Angiogenesis and Lymphangiogenesis Inhibition in Cancer Therapy
Authors: Jones Dennis
Published: March 2020

Metastasis is the primary cause of cancer-related mortality. Cancer cells primarily metastasize via blood and lymphatic vessels to colonize lymph nodes and distant organs, leading to worse prognosis. Thus, strategies to limit blood and lymphatic spread of cancer have been a focal point of cancer research for several decades. Resistance to FDA-approved anti-angiogenic therapies designed to limit blood vessel growth has emerged as a significant clinical challenge. However, there are no FDA-approved drugs that target tumor lymphangiogenesis, despite the consequences of metastasis through the lymphatic system. This review highlights several of the key resistance mechanisms to anti-angiogenic therapy and potential challenges facing anti-lymphangiogenic therapy. Blood and lymphatic vessels are more than just conduits for nutrient, fluid, and cancer cell transport. Recent studies have elucidated how these vasculatures often regulate immune responses. Vessels that are abnormal or compromised by tumor cells can lead to immunosuppression. Therapies designed to improve lymphatic vessel function while limiting metastasis may represent a viable approach to enhance immunotherapy and limit cancer progression.
On the TRAIL of Better Therapies: Understanding TNFRSF Structure-Function
Authors: Vanamee Éva S. , Faustman Denise L.
Published: March 2020

Tumor necrosis factor (TNF) superfamily ligands show diverse biological functions, such as the induction of apoptotic cell death or cell survival and proliferation, making them excellent therapeutic targets for cancer and autoimmunity. We review the latest literature on TNF receptor superfamily signaling with a focus on structure-function. Using combinatorics, we argue that receptors that cluster on the cell surface and are activated by membrane-bound ligands need to arrange in a highly ordered manner, as the probability of random ligand and receptor arrangements matching up for receptor activation is very low. A growing body of evidence indicates that antiparallel receptor dimers that sequester the ligand binding site cluster on the cell surface, forming a hexagonal lattice. Upon ligand binding, this arrangement puts the activated receptors at the right distance to accommodate the downstream signaling partners. The data also suggest that the same geometry is utilized regardless of receptor type. The unified model provides important clues about TNF receptor signaling and should aid the design of better therapies for cancer and various immune mediated diseases.
The Transport and Outcome of Sick Outborn Neonates Admitted to a Regional and District Hospital in the Upper West Region of Ghana: A Cross-Sectional Study
Authors: Tette Edem M. A. , Nuertey Benjamin D. , Akaateba Dominic , Gandau Naa Barnabas
Published: March 2020

Optimum care of sick neonates often involves transporting them across different levels of care. Since their condition may deteriorate over time, attention needs to be paid to travel distances and how they are transferred. We examined the mode of transport, distances travelled, condition on arrival and outcome of outborn neonates admitted to a district and a regional hospital in Ghana using a cross-sectional study involving caregivers of neonates admitted to these hospitals. Information on referral characteristics and outcome were obtained from questionnaires and the child’s case notes. Overall, 153 caregivers and babies were studied. Twelve deaths, 7.8%, occurred. Neonates who died spent a median duration of 120 min at the first health facility they visited compared with 30 min spent by survivors; they travelled mostly by public buses, (41.7%), compared with 36.0% of survivors who used taxis. Majority of survivors, 70.2%, had normal heart rates on arrival compared with only 41.7% of neonates who died; hypothermia was present in 66.7% compared with 47.6% of survivors. These findings indicate that the logistics for neonatal transport were inadequate to keep the neonates stable during the transfer process, thus many of them were compromised especially those who died. Further studies are warranted.
Improved Thermal Processing of Polylactic Acid/Oxidized Starch Composites and Flame-Retardant Behavior of Intumescent Non-Wovens
Authors: Maqsood Muhammad , Seide Gunnar
Published: March 2020

Thermoplastic processing and spinning of native starch is very challenging due to (a) the linear and branched polymers (amylose and amylopectin) present in its structure and (b) the presence of inter-and-intramolecular hydrogen bond linkages in its macromolecules that restrict the molecular chain mobility. Therefore, in this study, oxidized starch (OS) (obtained after oxidation of native starch with sodium perborate) was melt-blended with polylactic acid (PLA) polymer to prepare PLA/OS blends that were then mixed together with ammonium polyphosphate (APP), a halogen-free flame retardant (FR) used as acid donor in intumescent formulations on twin-screw extruder to prepare PLA/OS/APP composites. OS with different concentrations also served as bio-based carbonic source in intumescent formulations. PLA/OS/APP composites were melt spun to multifilament fibers on pilot scale melt-spinning machine and their crystallinity and mechanical properties were optimized by varying spinning parameters. The crystallinity of the fibers was studied by differential scanning calorimetry and thermal stabilities were analyzed by thermogravimetric analysis. Scanning electron microscopy was used to investigate the surface morphology and dispersion of the additives in the fibers. Needle-punched non-woven fabrics from as prepared melt-spun PLA/OS/APP fibers were developed and their fire properties such as heat release rate, total heat release, time to ignition, residual mass % etc. by cone calorimetry test were measured. It was found that PLA/OS/APP composites can be melt spun to multifilament fibers and non-woven flame-retardant fabrics produced thereof can be used in industrial FR applications.
Singular Mean-Field States: A Brief Review of Recent Results
Authors: Shamriz Elad , Chen Zhaopin , Malomed Boris A. , Sakaguchi Hidetsugu
Published: March 2020

This article provides a focused review of recent findings which demonstrate, in some cases quite counter-intuitively, the existence of bound states with a singularity of the density pattern at the center; the states are physically meaningful because their total norm converges. One model of this type is based on the 2D Gross–Pitaevskii equation (GPE), which combines the attractive potential ∼ r − 2 and the quartic self-repulsive nonlinearity, induced by the Lee–Huang–Yang effect (quantum fluctuations around the mean-field state). The GPE demonstrates suppression of the 2D quantum collapse, driven by the attractive potential, and emergence of a stable ground state (GS), whose density features an integrable singularity ∼ r − 4 / 3 at r → 0 . Modes with embedded angular momentum exist too, but they are unstable. A counter-intuitive peculiarity of the model is that the GS exists even if the sign of the potential is reversed from attraction to repulsion, provided that its strength is small enough. This peculiarity finds a relevant explanation. The other model outlined in the review includes 1D, 2D, and 3D GPEs, with the septimal (seventh-order), quintic, and cubic self-repulsive terms, respectively. These equations give rise to stable singular solitons, which represent the GS for each dimension D, with the density singularity ∼ r − 2 / ( 4 − D ) . Such states may be considered the results of screening a “bare” delta-functional attractive potential by the respective nonlinearities.
Towards an Optimal Sample Delivery Method for Serial Crystallography at XFEL
Authors: Cheng Robert
Published: March 2020

The advent of the X-ray free electron laser (XFEL) in the last decade created the discipline of serial crystallography but also the challenge of how crystal samples are delivered to X-ray. Early sample delivery methods demonstrated the proof-of-concept for serial crystallography and XFEL but were beset with challenges of high sample consumption, jet clogging and low data collection efficiency. The potential of XFEL and serial crystallography as the next frontier of structural solution by X-ray for small and weakly diffracting crystals and provision of ultra-fast time-resolved structural data spawned a huge amount of scientific interest and innovation. To utilize the full potential of XFEL and broaden its applicability to a larger variety of biological samples, researchers are challenged to develop better sample delivery methods. Thus, sample delivery is one of the key areas of research and development in the serial crystallography scientific community. Sample delivery currently falls into three main systems: jet-based methods, fixed-target chips, and drop-on-demand. Huge strides have since been made in reducing sample consumption and improving data collection efficiency, thus enabling the use of XFEL for many biological systems to provide high-resolution, radiation damage-free structural data as well as time-resolved dynamics studies. This review summarizes the current main strategies in sample delivery and their respective pros and cons, as well as some future direction.
Modeling the Local Deformation and Transformation Behavior of Cast X8CrMnNi16-6-6 TRIP Steel and 10% Mg-PSZ Composite Using a Continuum Mechanics-Based Crystal Plasticity Model
Authors: Qayyum Faisal , Guk Sergey , Schmidtchen Matthias , Kawalla Rudolf , Prahl Ulrich
Published: March 2020

A Transformation-Induced Plasticity (TRIP) steel matrix reinforced with magnesium-partially stabilized zirconia (Mg-PSZ) particles depicts a superior energy absorbing capacity during deformation. In this research, the TRIP/TWIP material model already developed in the framework of the Düsseldorf Advanced Material Simulation Kit (DAMASK) is tuned for X8CrMnNi16-6-6 TRIP steel and 10% Mg-PSZ composite. A new method is explained to more accurately tune this material model by comparing the stress/strain, transformation, twinning, and dislocation glide obtained from simulations with respective experimental acoustic emission measurements. The optimized model with slight modification is assigned to the steel matrix in 10% Mg-PSZ composite material. In the simulation model, zirconia particles are assigned elastic properties with a perfect ceramic/matrix interface. Local deformation, transformation, and the twinning behavior of the steel matrix due to quasi-static tensile load were analyzed. The comparison of the simulation results with acoustic emission data shows good correlation and helps correlate acoustic events with physical attributes. The tuned material models are used to run full phase simulations using 2D Electron Backscatter Diffraction (EBSD) data from steel and 10% Mg-PSZ zirconia composites. Form these simulations, dislocation glide, martensitic transformation, stress evolution, and dislocation pinning in different stages of deformation are qualitatively discussed for the steel matrix and ceramic inclusions.
Spinel to Rock-Salt Transformation in High Entropy Oxides with Li Incorporation
Authors: Wang Junbo , Stenzel David , Azmi Raheleh , Najib Saleem , Wang Kai , Jeong Jaehoon , Sarkar Abhishek , Wang Qingsong , Sukkurji Parvathy Anitha , Bergfeldt Thomas , Botros Miriam , Maibach Julia , Hahn Horst , Brezesinski Torsten , Breitung Ben
Published: March 2020

High entropy oxides (HEOs) constitute a promising class of materials with possibly new and largely unexplored properties. The virtually infinite variety of compositions (multi-element approach) for a single-phase structure allows the tailoring of their physical properties and enables unprecedented materials design. Nevertheless, this level of versatility renders their characterization as well as the study of specific processes or reaction mechanisms challenging. In the present work, we report the structural and electrochemical behavior of different multi-cationic HEOs. Phase transformation from spinel to rock-salt was observed upon incorporation of monovalent Li+ ions, accompanied by partial oxidation of certain elements in the lattice. This transition was studied by X-ray diffraction, inductively coupled plasma-optical emission spectroscopy, X-ray photoelectron spectroscopy, transmission electron microscopy, and attenuated total reflection infrared spectroscopy. In addition, the redox behavior was probed using cyclic voltammetry. Especially, the lithiated rock-salt structure HEOs were found to exhibit potential for usage as negative and positive electrode materials in rechargeable lithium-ion batteries.
Development of Fashion Product Retrieval and Recommendations Model Based on Deep Learning
Authors: Jo Jaechoon , Lee Seolhwa , Lee Chanhee , Lee Dongyub , Lim Heuiseok
Published: March 2020

The digitization of the fashion industry diversified consumer segments, and consumers now have broader choices with shorter production cycles; digital technology in the fashion industry is attracting the attention of consumers. Therefore, a system that efficiently supports the searching and recommendation of a product is becoming increasingly important. However, the text-based search method has limitations because of the nature of the fashion industry, in which design is a very important factor. Therefore, we developed an intelligent fashion technique based on deep learning for efficient fashion product searches and recommendations consisting of a Sketch-Product fashion retrieval model and vector-based user preference fashion recommendation model. It was found that the “Precision at 5” of the image-based similar product retrieval model was 0.774 and that of the sketch-based similar product retrieval model was 0.445. The vector-based preference fashion recommendation model also showed positive performance. This system is expected to enhance consumers’ satisfaction by supporting users in more effectively searching for fashion products or by recommending fashion products before they begin a search.
Pattern Recognition Techniques for the Identification of Activities of Daily Living Using a Mobile Device Accelerometer
Authors: Pires Ivan Miguel , Marques Gonçalo , Garcia Nuno M. , Flórez-Revuelta Francisco , Canavarro Teixeira Maria , Zdravevski Eftim , Spinsante Susanna , Coimbra Miguel
Published: March 2020

The application of pattern recognition techniques to data collected from accelerometers available in off-the-shelf devices, such as smartphones, allows for the automatic recognition of activities of daily living (ADLs). This data can be used later to create systems that monitor the behaviors of their users. The main contribution of this paper is to use artificial neural networks (ANN) for the recognition of ADLs with the data acquired from the sensors available in mobile devices. Firstly, before ANN training, the mobile device is used for data collection. After training, mobile devices are used to apply an ANN previously trained for the ADLs’ identification on a less restrictive computational platform. The motivation is to verify whether the overfitting problem can be solved using only the accelerometer data, which also requires less computational resources and reduces the energy expenditure of the mobile device when compared with the use of multiple sensors. This paper presents a method based on ANN for the recognition of a defined set of ADLs. It provides a comparative study of different implementations of ANN to choose the most appropriate method for ADLs identification. The results show the accuracy of 85.89% using deep neural networks (DNN).
Electromagnetic Susceptibility of Battery Management Systems’ ICs for Electric Vehicles: Experimental Study
Authors: Aiello Orazio
Published: March 2020

The paper deals with the susceptibility to electromagnetic interference (EMI) of battery management systems (BMSs) for Li-ion and lithium-polymer (LiPo) battery packs employed in emerging electric and hybrid electric vehicles. A specific test board was developed to experimentally assess the EMI susceptibility of a BMS front-end integrated circuit by direct power injection (DPI) and radiated susceptibility measurements in an anechoic chamber. Experimental results are discussed in reference to the different setup, highlighting the related EMI-induced failure mechanisms observed during the tests.
A Mathematical Tightening of Instantaneous Indoor and Outdoor Dry-Bulb and Wet-Bulb Temperature Tolerances
Authors: Hu Xinhao , Zhang Zhongbin , Cai Dandan
Published: March 2020

To reduce the inaccuracy in energy efficiency evaluation, indoor and outdoor dry-bulb and wet-bulb temperature (D&WBT) tolerances in the currently enacted Chinese room air conditioner (RAC) national standard were tightened into narrower intervals in this investigation. Characteristics of cooling capacity (CC) and energy efficiency ratio (EER) changing with D&WBTs were analyzed based on performance tests. AICc (corrected Akaike Information Criterion) was applied to determine the best fitted 3D curve equations of CC and EER to find the intervals of gradient extrema of CC and EER. The corresponding intervals of indoor and outdoor D&WBTs were concluded as the tightened D&WBT tolerances. For illustration, based on the performance tests of 154 working conditions, the instantaneous indoor and outdoor D&WBT tolerances in nominal refrigerating working conditions were respectively tightened from the original ±0.5/±0.3 °C into ±0.3/±0.2 °C (indoor tolerances) and ±0.4/±0.3 °C (outdoor tolerances). EER variation rate thus decreased from 2.11% to 1.03% (indoor) and 2.11% to 1.25% (outdoor).
Considered Factors of Online News Based on Respondents’ Eye Activity Using Eye-Tracker Analysis
Authors: Yohandy Daniel Hadrian , Setyohadi Djoko Budiyanto , Santoso Albertus Joko
Published: March 2020

Development of the internet as a source of information has penetrated many aspects of human life, which is shown in the increasingly diverse substance of news in online news sources. Previous studies have stated that the presentation of the substance of online news information can have negative impacts, especially the emergence of anxiety in users; thus, managing the presentation of information becomes important. This study intends to explore factors that should be considered as possible anxiety-inducers for readers of news sites. Analyses of areas of interest (AOIs), fixation, and heat maps from respondents’ eye activity obtained from eye-tracker data have been compiled with Beck Anxiety Inventory (BAI) measurement results to analyze anxiety among newsreaders. The results show that text is the dominant center of attention in various types of news. The reason for the higher anxiety that arises from text on online news sites is twofold. First, there are the respondents’ experiences. Second, text usage allows for boundless possibilities in respondents’ imaginations as a response to the news that has occurred.
Antimicrobial Activity and Mechanism of Action of the Amaranthus tricolor Crude Extract against Staphylococcus aureus and Potential Application in Cooked Meat
Authors: Guo Ling , Wang Yanyan , Bi Xue , Duo Kai , Sun Qi , Yun Xueqi , Zhang Yibo , Fei Peng , Han Jianchun
Published: March 2020

Amaranthus tricolor has been reported to contain some antimicrobial compounds, such as alkaloids, polyphenols, and terpenoids. However, its effect on Staphylococcus aureus has been less well researched. Therefore, this study was designed to evaluate the antimicrobial activity and possible mechanism of action of the Amaranthus tricolor crude extract (ATCE) against S. aureus and potential application in cooked meat. The antimicrobial activity against S. aureus was assessed by disk diffusion, minimum inhibitory concentration (MIC) determinations, and growth curve. The changes of bacterial membrane potential, intracellular pH (pHin), content of bacterial protein and DNA, and cell morphology were measured to indicate its antimicrobial mechanism of action. The effects of different concentrations of ATCE on bacterial counts, pH, and color of lean cooked pork during 6 d storage were assessed. The results showed that the diameter of inhibition zone (DIZ) and MIC of ATCE against S. aureus were 12.63 ± 0.34 to 12.94 ± 0.43 mm and 80 mg/mL, respectively. The mechanism of action of ATCE against S. aureus was associated with cell membrane depolarization, reduction of pHin, decrease of bacterial protein content, cleavage of cell DNA, and leakage of cytoplasm. Besides, ATCE resulted in a reduction of 1.02 log CFU/g from 3 log CFU/g in S. aureus-inoculated lean cooked pork. The pH values of lean cooked pork treated with ATCE did not show significant changes as the storage time increased, but there was a slight and significant decrease seen with the application of 1 and 2 MIC of ATCE. After treating with ATCE, the color of lean cooked pork showed less lightness (L*), more redness (a∗), similar yellowness (b*), stronger chroma (C*), and weaker hue angle (h*) during 6 days of storage. Therefore, these findings indicate that ATCE has antimicrobial activities against S. aureus and possesses latent energy to become a natural preservative to maintain the quality of lean cooked pork.
Are Faba Bean and Pea Proteins Potential Whey Protein Substitutes in Infant Formulas? An In Vitro Dynamic Digestion Approach
Authors: Le Roux Linda , Ménard Olivia , Chacon Raphaël , Dupont Didier , Jeantet Romain , Deglaire Amélie , Nau Françoise
Published: March 2020

Infant formulas (IFs) are used as substitutes for human milk and are mostly based on cow milk proteins. For sustainability reasons, animal protein alternatives in food are increasingly being considered, as plant proteins offer interesting nutritional and functional benefits for the development of innovative IFs. This study aimed to assess how a partial substitution (50%) of dairy proteins with faba bean and pea proteins influenced the digestibility of IFs under simulated dynamic in vitro digestion, which were set up to mimic infant digestion. Pea- and faba bean-based IFs (PIF and FIF, respectively) have led to a faster aggregation than the reference milk-based IF (RIF) in the gastric compartment; that did not affect the digesta microstructure at the end of digestion. The extent of proteolysis was estimated via the hydrolysis degree, which was the highest for FIF (73%) and the lowest for RIF (50%). Finally, it was apparent that in vitro protein digestibility and protein digestibility-corrected amino acid score (PDCAAS)-like scores were similar for RIF and FIF (90% digestibility; 75% PDCAAS), but lower for PIF (75%; 67%). Therefore, this study confirms that faba bean proteins could be a good candidate for partial substitution of whey proteins in IFs from a nutritional point of view, provided that these in vitro results are confirmed in vivo.
Outpatient Waiting Time at Vietnam Health Facilities: Policy Implications for Medical Examination Procedure
Authors: Nguyen Dinh-Hoa , Tran Dinh-Van , Vo Hoang-Long , Nguyen Si Anh Hao , Doan Thi-Ngoc-Ha , Nguyen Thi-Huyen-Trang
Published: March 2020

Our study aims to measure outpatient waiting times at Vietnam health facilities according to the socioeconomic characteristics. We employed the 2015 Vietnam District and Commune Health Facility Survey which was a cross-sectional study designed by the World Bank in collaboration with the Vietnam Health Strategy and Policy Institute. This survey was designed to be representative of six provinces (Dien Bien, Hanoi, Binh Dinh, Dak Lak, Dong Nai, and Dong Thap) drawn from six distinct geographical regions of Vietnam. Data from 4949 outpatients at district hospitals (DHs) and 1724 outpatients at commune health centers (CHCs) were extracted for final analysis. We recorded average outpatient waiting times of 32.58 min at DHs and of 11.58 min at CHCs. Four hundred and forty-five outpatients at DHs (9.0%) and 720 those at CHCs (42.8%) were examined immediately (waiting time = 0 min). Outpatient waiting times were various in six distinct geographical regions. With an investigation according to several socioeconomic characteristics, significant differences in outpatient waiting times were observed at both two levels of health facilities as measured by province, age, self-reported health status, patient’s wealth, ethnicity, and health insurance. Conclusions. Outpatient waiting times from arrival at health facility until receiving care were significantly distinct amongst two health facility levels, revealing longer at DHs compared to at CHCs. There was significantly higher proportion of outpatients examined immediately at CHCs compared to at DHs. Our study suggests that, vulnerable populations, with longer outpatient waiting time, should be dealt with in appropriate models towards each medical facility according to key socioeconomic factors to contribute to simplify the process of medical examination and treatment for outpatients.
Knowledge about Microplastic in Mediterranean Tributary River Ecosystems: Lack of Data and Research Needs on Such a Crucial Marine Pollution Source
Authors: Guerranti Cristiana , Perra Guido , Martellini Tania , Giari Luisa , Cincinelli Alessandra
Published: March 2020

Plastic debris occurring in freshwater environments, which can either come from the surrounding terrestrial areas or transported from upstream, has been identified as one of the main sources and routes of plastic pollution in marine systems. The ocean is the final destination of land- based microplastic sources, but compared to marine environments, the occurrence and effects of microplastics in freshwater ecosystems remain largely unknown. A thorough examination of scientific literature on abundance, distribution patterns, and characteristics of microplastics in freshwater environments in Mediterranean tributary rivers has shown a substantial lack of information and the need to apply adequate and uniform measurement methods.
High-Rate Laser Surface Texturing for Advanced Tribological Functionality
Authors: Schille Jörg , Schneider Lutz , Mauersberger Stefan , Szokup Sylvia , Höhn Sören , Pötschke Johannes , Reiß Friedemann , Leidich Erhard , Löschner Udo
Published: March 2020

This article features with the enhancement of the static coefficient of friction by laser texturing the contact surfaces of tribological systems tested under dry friction conditions. The high-rate laser technology was applied for surface texturing at unprecedented processing rates, namely using powerful ultrashort pulses lasers in combination with ultrafast polygon-mirror based scan systems. The laser textured surfaces were analyzed by ion beam slope cutting and Raman measurements, showing a crystallographic disordering of the produced microscopic surface features. The laser induced self-organizing periodic surface structures as well as deterministic surface textures were tested regarding their tribological behavior. The highest static coefficient of friction was found of µ20 = 0.68 for a laser textured cross pattern that is 126% higher than for a fine grinded reference contact system. The line pattern was textured on a shaft-hub connection where the static coefficient of friction increased up to 75% that demonstrates the high potential of the technology for real-world applications.
A Hybrid Forward–Backward Algorithm and Its Optimization Application
Authors: Liu Liya , Qin Xiaolong , Yao Jen-Chih
Published: March 2020

In this paper, we study a hybrid forward–backward algorithm for sparse reconstruction. Our algorithm involves descent, splitting and inertial ideas. Under suitable conditions on the algorithm parameters, we establish a strong convergence solution theorem in the framework of Hilbert spaces. Numerical experiments are also provided to illustrate the application in the field of signal processing.
A Novel Method of Optimal Capacitor Placement in the Presence of Harmonics for Power Distribution Network Using NSGA-II Multi-Objective Genetic Optimization Algorithm
Authors: Ebrahimi Moghadam Majid , Falaghi Hamid , Farhadi Mahdi
Published: March 2020

One of the effective ways of reducing power system losses is local compensation of part of the reactive power consumption by deploying shunt capacitor banks. Since the capacitor’s impedance is frequency-dependent and it is possible to generate resonances at harmonic frequencies, it is important to provide an efficient method for the placement of capacitor banks in the presence of nonlinear loads which are the main cause of harmonic generation. This paper proposes a solution for a multi-objective optimization problem to address the optimal placement of capacitor banks in the presence of nonlinear loads, and it establishes a reasonable reconciliation between costs, along with improvement of harmonic distortion and a voltage index. In this paper, while using the harmonic power flow method to calculate the electrical quantities of the grid in terms of harmonic effects, the non-dominated sorting genetic (NSGA)-II multi-objective genetic optimization algorithm was used to obtain a set of solutions named the Pareto front for the problem. To evaluate the effectiveness of the proposed method, the problem was tested for an IEEE 18-bus system. The results were compared with the methods used in eight other studies. The simulation results show the considerable efficiency and superiority of the proposed flexible method over other methods.
Alarmins in Osteoporosis, RAGE, IL-1, and IL-33 Pathways: A Literature Review
Authors: De Martinis Massimo , Ginaldi Lia , Sirufo Maria Maddalena , Pioggia Giovanni , Calapai Gioacchino , Gangemi Sebastiano , Mannucci Carmen
Published: March 2020

Alarmins are endogenous mediators released by cells following insults or cell death to alert the host’s innate immune system of a situation of danger or harm. Many of these, such as high-mobility group box-1 and 2 (HMGB1, HMGB2) and S100 (calgranulin proteins), act through RAGE (receptor for advanced glycation end products), whereas the IL-1 and IL-33 cytokines bind the IL-1 receptors type I and II, and the cellular receptor ST2, respectively. The alarmin family and their signal pathways share many similarities of cellular and tissue localization, functions, and involvement in various physiological processes and inflammatory diseases including osteoporosis. The aim of the review was to evaluate the role of alarmins in osteoporosis. A bibliographic search of the published scientific literature regarding the role of alarmins in osteoporosis was organized independently by two researchers in the following scientific databases: Pubmed, Scopus, and Web of Science. The keywords used were combined as follows: “alarmins and osteoporosis”, “RAGE and osteoporosis”, “HMGB1 and osteoporosis”, “IL-1 and osteoporosis”, “IL 33 and osteopororsis”, “S100s protein and osteoporosis”. The information was summarized and organized in the present review. We highlight the emerging roles of alarmins in various bone remodeling processes involved in the onset and development of osteoporosis, as well as their potential role as biomarkers of osteoporosis severity and progression. Findings of the research suggest a potential use of alarmins as pharmacological targets in future therapeutic strategies aimed at preventing bone loss and fragility fractures induced by aging and inflammatory diseases.
Maxillary Implant-Supported Overdentures: Mechanical Behavior Comparing Individual Axial and Bar Retention Systems. A Cohort Study of Edentulous Patients
Authors: Mañes Ferrer José Félix , Fernández-Estevan Lucía , Selva-Otaolaurruchi Eduardo , Labaig-Rueda Carlos , Solá-Ruíz María Fernanda , Agustín-Panadero Rubén
Published: March 2020

Background and objectives: To compare the medium- to long-term mechanical behavior of overdentures with two different retention systems: overdentures with Locator® axial retention, and vertical insertion overdentures with bar retention, used to rehabilitate edentulous maxillar. Material and Methods: This prospective study assessed patients presenting complete maxillary edentulism, rehabilitated by means of implant-supported overdentures (n = 20), 10 with Locator® axial retention (ODA group) and 10 with overdentures on bars (ODB group). Patients also completed a questionnaire to determine their satisfaction with treatment. Results: The mean follow-up time in both groups was 11.4 years, with follow-up times in both groups ranging from 5 to 14 years. The ODA group suffered mechanical complications such as retention loss, need for nylon retention insert changes, resin fracture, and need for relining. In the ODB group, prosthetic dental wear, screw loosening, and complete prosthetic failure were more common. A total of 19 implants failed (23.8%); of these, 11 were in the ODA group (failure rate = 27.5%) and eight in the ODB group (failure rate = 20%). The patient satisfaction questionnaire obtained a mean score of 7.9 out of 10 in the ODA group, and 9.75 in the ODB group. Conclusions: in rehabilitations of edentulous maxillar by means of implant-supported overdentures, both the systems assessed were shown to be effective in the medium to long term. Patients expressed a high level of satisfaction with the treatments received.
Thermal and Mass Spectroscopic Analysis of BF and BOF Sludges: Study of Their Behavior under Air and Inert Atmosphere
Authors: Omran Mamdouh , Fabritius Timo , Yu Yaowei , Chen Guo
Published: March 2020

Differential thermal analysis (DTA), thermogravimetry (TG), and mass spectrometry (MS) were used to study the thermal behavior of the blast furnace (BF) and basic oxygen furnace (BOF) sludges generated from the iron-making industry. The results indicated that under air atmosphere the two types of sludge are different in their thermal behavior. In BF sludge, the exothermic carbon gasification (CO/CO2) reaction dominated the process, while in BOF sludge, the significant reaction occurred at 755 °C and was associated with a slight mass gain owing to the partial oxidation of Fe3O4 to Fe2O3. Under inert atmosphere, the thermal behavior of both BF and BOF sludges were dominated by a reduction reaction. In BF sludge, the endothermic reactions ranged from 785 to 1115 °C due to the reduction of iron oxides as follows: Fe2O3 → Fe3O4 → FeO → Fe. A total mass loss of about 27.78% was observed in the TG curve. While in BOF sludge, the endothermic peaks corresponded to magnetite reduction to iron (Fe). The overall mass loss of the BOF was approximately 16.92%. The mass spectrum of gases evolution for both BF and BOF sludges revealed that CO/CO2 gases were released from the sludges.
Protective Role of Vitamin D in Renal Tubulopathies
Authors: Gembillo Guido , Cernaro Valeria , Siligato Rossella , Curreri Francesco , Catalano Antonino , Santoro Domenico
Published: March 2020

Vitamin D is tightly linked with renal tubular homeostasis: the mitochondria of proximal convoluted tubule cells are the production site of 1α,25-dihydroxyvitamin D3. Patients with renal impairment or tubular injury often suffer from chronic inflammation. This alteration comes from oxidative stress, acidosis, decreased clearance of inflammatory cytokines and stimulation of inflammatory factors. The challenge is to find the right formula for each patient to correctly modulate the landscape of treatment and preserve the essential functions of the organism without perturbating its homeostasis. The complexity of the counter-regulation mechanisms and the different axis involved in the Vitamin D equilibrium pose a major issue on Vitamin D as a potential effective anti-inflammatory drug. The therapeutic use of this compound should be able to inhibit the development of inflammation without interfering with normal homeostasis. Megalin-Cubilin-Amnionless and the FGF23-Klotho axis represent two Vitamin D-linked mechanisms that could modulate and ameliorate the damage response at the renal tubular level, balancing Vitamin D therapy with an effect potent enough to contrast the inflammatory cascades, but which avoids potential severe side effects.
Nematode-Trapping Fungi Produce Diverse Metabolites during Predator–Prey Interaction
Authors: Kuo Ting-Hao , Yang Ching-Ting , Chang Hsin-Yuan , Hsueh Yen-Ping , Hsu Cheng-Chih
Published: March 2020

Nematode-trapping fungi are natural antagonists of nematodes. These predatory fungi are capable of switching their lifestyle from a saprophytic to predatory stage in the presence of nematodes by developing specialized trapping devices to capture and consume nematodes. The biochemical mechanisms of such predator–prey interaction have become increasingly studied given the potential application of nematode-trapping fungi as biocontrol agents, but the involved fungal metabolites remain underexplored. Here, we report a comprehensive liquid–chromatography mass spectrometry (LC–MS) metabolomics study on one hundred wild isolates of nematode-trapping fungi in three different species, Arthrobotrys oligospora, Arthrobotrys thaumasia, and Arthrobotrys musiformis. Molecular networking analysis revealed that the fungi were capable of producing thousands of metabolites, and such chemical diversity of metabolites was notably increased as the fungi switched lifestyle to the predatory stage. Structural annotations by tandem mass spectrometry revealed that those fungal metabolites belonged to various structural families, such as peptide, siderophore, fatty alcohol, and fatty acid amide, and their production exhibited species specificity. Several small peptides (<1.5 kDa) produced by A. musiformis in the predatory stage were found, with their partial amino acid sequences resolved by the tandem mass spectra. Four fungal metabolites (desferriferrichrome, linoleyl alcohol, nonadecanamide, and citicoline) that were significantly enriched in the predatory stage were identified and validated by chemical standards, and their bioactivities against nematode prey were assessed. The availability of the metabolomics datasets will facilitate comparative studies on the metabolites of nematode-trapping fungi in the future.
An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries
Authors: Patel Alok , Karageorgou Dimitra , Rova Emma , Katapodis Petros , Rova Ulrika , Christakopoulos Paul , Matsakas Leonidas
Published: March 2020

Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.
Application of an O-Linked Glycosylation System in Yersinia enterocolitica Serotype O:9 to Generate a New Candidate Vaccine against Brucella abortus
Authors: Huang Jing , Pan Chao , Sun Peng , Feng Erling , Wu Jun , Zhu Li , Wang Hengliang
Published: March 2020

Brucellosis is a major zoonotic public health threat worldwide, causing veterinary morbidity and major economic losses in endemic regions. However, no efficacious brucellosis vaccine is yet available, and live attenuated vaccines commonly used in animals can cause human infection. N- and O-linked glycosylation systems have been successfully developed and exploited for the production of successful bioconjugate vaccines. Here, we applied an O-linked glycosylation system to a low-pathogenicity bacterium, Yersinia enterocolitica serotype O:9 (Y. enterocolitica O:9), which has repeating units of O-antigen polysaccharide (OPS) identical to that of Brucella abortus (B. abortus), to develop a bioconjugate vaccine against Brucella. The glycoprotein we produced was recognized by both anti-B. abortus and anti-Y. enterocolitica O:9 monoclonal antibodies. Three doses of bioconjugate vaccine-elicited B. abortus OPS-specific serum IgG in mice, significantly reducing bacterial loads in the spleen following infection with the B. abortus hypovirulent smooth strain A19. This candidate vaccine mitigated B. abortus infection and prevented severe tissue damage, thereby protecting against lethal challenge with A19. Overall, the results indicated that the bioconjugate vaccine elicited a strong immune response and provided significant protection against brucellosis. The described vaccine preparation strategy is safe and avoids large-scale culture of the highly pathogenic B. abortus.
Bastnaesite, Barite, and Calcite Flotation Behaviors with Salicylhydroxamic Acid as the Collector
Authors: Xiong Wenliang , Deng Jie , Zhao Kaile , Wang Weiqing , Wang Yanhong , Wei Dezhou
Published: March 2020

The flotation of bastnaesite, as a major mineral source of rare earth elements, attracting much attention in the mineral processing field, is challenging owing to the natural flotability of calcium-bearing minerals. To promote the application of flotation, we systematically investigated the flotation behavior of bastnaesite, barite, and calcite, with salicylhydroxamic acid (SHA) as the collector through micro-flotation experiments, zeta-potential measurements, Fourier transform infrared (FT-IR) analyses, X-ray photoelectron spectroscopy (XPS) analyses, and solution chemistry analyses. Micro-flotation experiments confirm that the flotability of bastnaesite is high at pH 6.5–8.5, while calcite floats at pH 8.0–9.5, and barite has little flotation response. The results of FT-IR, XPS, and zeta-potential measurements indicate that there is chemical adsorption of SHA on the bastnaesite surface, and physical adsorption also occurs. However, as for barite and calcite, there is only physical adsorption of SHA on the surfaces. The solution chemistry results show that SHA anions can interact with RE3+, REOH2+, and RE(OH)2+ on bastnaesite surfaces in aqueous suspensions, resulting in bastnaesite flotation.