bluuebunny/crossref_metadata_2025 huggingface dataset:
Needs-Assessment for an Artificial Intelligence-Based Chatbot for Pharmacists in HIV Care: Results from a Knowledge–Attitudes–Practices Survey
Authors: Laymouna Moustafa , Ma Yuanchao , Lessard David , Engler Kim , Therrien Rachel , Schuster Tibor , Vicente Serge , Achiche Sofiane , El Haj Maria Nait , Lemire Benoît , Kawaiah Abdalwahab , Lebouché Bertrand
Published: August 2024

Background: Pharmacists need up-to-date knowledge and decision-making support in HIV care. We aim to develop MARVIN-Pharma, an adapted artificial intelligence-based chatbot initially for people with HIV, to assist pharmacists in considering evidence-based needs. Methods: From December 2022 to December 2023, an online needs-assessment survey evaluated Québec pharmacists’ knowledge, attitudes, involvement, and barriers relative to HIV care, alongside perceptions relevant to the usability of MARVIN-Pharma. Recruitment involved convenience and snowball sampling, targeting National HIV and Hepatitis Mentoring Program affiliates. Results: Forty-one pharmacists (28 community, 13 hospital-based) across 15 Québec municipalities participated. Participants perceived their HIV knowledge as moderate (M = 3.74/6). They held largely favorable attitudes towards providing HIV care (M = 4.02/6). They reported a “little” involvement in the delivery of HIV care services (M = 2.08/5), most often ART adherence counseling, refilling, and monitoring. The most common barriers reported to HIV care delivery were a lack of time, staff resources, clinical tools, and HIV information/training, with pharmacists at least somewhat agreeing that they experienced each (M ≥ 4.00/6). On average, MARVIN-Pharma’s acceptability and compatibility were in the ‘undecided’ range (M = 4.34, M = 4.13/7, respectively), while pharmacists agreed to their self-efficacy to use online health services (M = 5.6/7). Conclusion: MARVIN-Pharma might help address pharmacists’ knowledge gaps and barriers to HIV treatment and care, but pharmacist engagement in the chatbot’s development seems vital for its future uptake and usability.
Path to Suicidality in Korean Adolescents: Mediating Role of Self-Esteem, Somatic Symptoms, and Self-Harm Amid Depressive Symptoms
Authors: Kim Jiyeon , Cho Myongsun
Published: August 2024

This study explored the relationship between depressive symptoms and suicidality among community-dwelling adolescents aged 10–18 years, examining whether self-esteem, somatic symptoms, and self-harm mediate this relationship. Utilizing a pre-existing dataset from a nationwide adolescent mental health survey conducted in Korea in 2021, data were collected using several standardized self-administered instruments: the Korean version of Rosenberg’s self-esteem scale, Korean Children’s Somatization Inventory, Korean version of the Self-Harm Inventory, Mental Health Screening for Depressive Disorders, and Mental Health Screening for Suicide Risk. A path model was constructed and validated, followed by path analysis to assess the effects. Data from 6689 adolescents, including 5937 students and 752 out-of-school adolescents, revealed that 18.7% were in the suicidality group, 11.8% experienced depressive symptoms, 57.9% exhibited somatic symptoms, and 27.4% engaged in self-harm. Depressive symptoms had a positive direct effect on suicidality (β = 0.166, p < 0.001, 95% confidence interval = 0.159–0.172). Bootstrapping tests showed a statistically significant indirect effect of self-esteem, somatic symptoms, and self-harm on the relationship between depressive symptoms and suicidality (β = 0.021, 95% confidence interval = 0.013–0.029). Our findings suggest that self-esteem, somatic symptoms, and self-harm mediate the relationship between depressive symptoms and suicidality, and comprehensive mental health management strategies addressing these factors are recommended.
It Is Not Just a Matter of Motivation: The Role of Self-Control in Promoting Physical Activity in Older Adults—A Bayesian Mediation Model
Authors: Camp Nicola , Vagnetti Roberto , Penner Samuel , Ramos Catarina , Hunter Kirsty , Hough John , Magistro Daniele
Published: August 2024

This study aimed to understand how psychological factors affect regular exercise in older adults, hypothesising that trait self-control mediates the relationship between motivation types (intrinsic, extrinsic, and amotivation) and exercise time. In this cross-sectional study, 430 older adults (mean age = 68.8 ± 6.72) completed questionnaires regarding their perceived trait self-control, motivation towards leisure activities, and level of physical activity. A Bayesian mediation analysis was performed, controlling for demographics. We documented positive direct (c′ = 0.021, 95%CI [0.001, 0.043]) and indirect (ab = 0.028, 95%CI [0.014, 0.043]) effects of intrinsic motivation on exercise, a fully mediated indirect effect of extrinsic motivation on exercise (ab = 0.027, 95%CI [0.011, 0.046]), and negative direct (c′ = −0.281, 95%CI [−0.368, −0.194]) and indirect (ab = −0.161, 95%CI [−0.221, −0.105]) effects of amotivation on exercise. There was no direct association between extrinsic motivation and exercise (c′ = 0.013, 95% CI [−0.013, 0.037]). In conclusion, trait self-control mediates motivation to influence exercise behaviour in older adults. Intrinsically motivated individuals resist sedentary living and show higher self-control, while extrinsically motivated ones rely on self-control and are more susceptible to non-adherence during mental fatigue. High amotivation is linked to less exercise and reduced self-control, suggesting potential non-compliance with structured exercise interventions.
Review of River Ice Observation and Data Analysis Technologies
Authors: Zakharov Igor , Puestow Thomas , Khan Amir Ali , Briggs Robert , Barrette Paul
Published: August 2024

This paper provides a comprehensive review of the available literature on the observation and characterization of river ice using remote sensing technologies. Through an analysis of 200 publications spanning from 1919 to June 2024, we reviewed different observation technologies deployed on in situ, aerial and satellite platforms for their utility in monitoring and characterizing river ice covers. River ice information, captured by 51 terms extracted from the literature, holds significant value in enhancing infrastructure resilience in the face of climate change. Satellite technologies, in particular the multispectral optical and multi-polarimetric synthetic aperture radar (SAR), provide a number of advantages, such as ice features discrimination, better ice characterization, and reliable delineation of open water and ice, with both current and upcoming sensors. The review includes data analysis methods employed for the monitoring and characterization of river ice, including ice information retrieval methods and corresponding accuracies. The need for further research on artificial intelligence and, in particular, deep learning (DL) techniques has been recognized as valuable for enhancing the accuracy of automated systems. The growing availability of freely available and commercial satellites, UAVs, and in situ data with improved characteristics suggests significant operational potential for river ice observation in the near future. Our study also identifies gaps in the current capabilities for river ice observation and provides suggestions for improved data analysis and interpretation.
Hemophagocytic Lymphohistiocytosis and Miliary Tuberculosis in an Apparently Immunocompetent Patient: A Case Report
Authors: Ducci Filippo , Mariotti Francesca , Mencarini Jessica , Fabbri Claudio , Manunta Alessandra Francesca , Messeri Daniela , Parronchi Paola , Blanc Pierluigi , Bartoloni Alessandro
Published: August 2024

Hemophagocytic lymphohistiocytosis (HLH) is a serious haematologic condition that can be related to various diseases, including tuberculosis (TB). The patient is a previously healthy 26-year-old man, originally from western Africa, admitted to hospital for fever and weight loss. Given the results of a computed tomography (CT) scan, ocular examination and microbiologic tests, miliary TB with pulmonary, lymph nodal and ocular involvement was diagnosed. Following the introduction of antitubercular treatment (ATT), an increase in inflammation indexes and severe pancytopenia were observed; at this point, the patient presented with six of the eight diagnostic criteria for HLH, and a diagnosis of HLH secondary to TB was raised. Therefore, HLH treatment with a high dose of dexamethasone was started, with a good clinical response. We performed a literature review of TB-related HLH, which shows a high mortality rate. ATT is necessary to ensure patient survival to remove the antigenic driver. Our patient developed HLH after the initiation of ATT as a paradoxical reaction, which may be linked to the release of antigens due to the bactericidal effect of ATT.
The Impact of COVID-19 Vaccination Side-Effects on Work Attendance among Saudi Healthcare Workers
Authors: Alguraini Jawaher , Saleem Mohamed T. S. , Mahrous Nahed N. , Shamsan Abbas , Zaidi Fatima Zia , Alhumaidan Ohoud S. , Jamous Yahya F.
Published: August 2024

Objective: This cross-sectional-survey-based study aimed to investigate the severity of side-effects from Coronavirus disease (COVID-19) mRNA (Pfizer, Moderna), viral vector DNA (Oxford-AstraZeneca, J&J/Janssen), inactivated virus (Sinopharm, Sinovac), and other vaccines among healthcare workers (HCWs) in Saudi Arabia, focusing on their impact on work attendance. Methods: A total of 894 HCWs residing in Saudi Arabia participated in this study from March 2023 to May 2023. Participants completed an online questionnaire assessing demographic information, vaccination status, comorbidities, vaccine side-effects, and missed work information after vaccination. Descriptive statistics and chi-square tests were used for data analysis. Results: The majority of participants were female (83.7%) and aged 25–34 years (42.8%). Most participants were predominantly vaccinated with mRNA vaccines. Common side-effects included pain at the injection site, fatigue, fever, and chills. However, no significant association was found between vaccine type, side-effects, and work absenteeism. While demographic factors such as age and healthcare profession did not influence work absenteeism, variations were observed among different racial groups. Conclusion: COVID-19 vaccination among HCWs in Saudi Arabia is associated with common side-effects, but their impact on work attendance is not significant. Understanding these implications can inform strategies to support the healthcare workforce and mitigate the impact on patient care and staffing during the ongoing COVID-19 pandemic.
The Effect of Inspiratory Muscle Training on Health-Related Fitness in College Students
Authors: Qin Lili , Liu Siyu , Hu Shuang , Feng Linlin , Wang Huan , Gong Xingzhou , Xuan Wei , Lu Tianfeng
Published: August 2024

In an era characterized by rapid economic growth and evolving lifestyles, college students encounter numerous challenges, encompassing academic pressures and professional competition. The respiratory muscle endurance capability is important for college students during prolonged aerobic exercise. Therefore, it is of great significance to explore an effective intervention to enhance the endurance level of college students. This study explores the transformative potential of inspiratory muscle training (IMT) to improve the physical functions of college students. This research comprised a group of 20 participants who underwent IMT integrated into their daily physical education classes or regular training sessions over an 8-week period, with 18 participants forming the control group. The IMT group adhered to the manufacturer’s instructions for utilizing the PowerBreathe device. The findings indicated a significant positive effect on inspiratory muscle strength (p < 0.001), showing improvements in pulmonary function, exercise tolerance, cardiac function, and overall athletic performance. These results revealed the substantial benefits of IMT in enhancing physical fitness and promoting health maintenance among college students.
Relative Health Risk Reduction from an Advanced Multi-Modal Air Purification System: Evaluation in a Post-Surgical Healthcare Setting
Authors: Pisaniello Dino , Nitschke Monika
Published: August 2024

Advanced air treatment systems have the potential to reduce airborne infection risk, improve indoor air quality (IAQ) and reduce energy consumption, but few studies reported practical implementation and performance. PlasmaShield®, an advanced multi-modal HVAC-integrated system, was directly compared with a standard MERV-13 system in a post-surgical paediatric healthcare setting. The evaluation entailed monitoring of multi-size airborne particles, bioaerosols and key IAQ parameters. Measurements were taken for outside air, supply air and air in the occupied space for 3 days prior to, and after, the installation of the PlasmaShield system. Compared with the existing arrangement, very significant reductions in particle number concentrations were observed in the occupied space, especially with virus-like submicron particles. Significant reductions in airborne culturable bacteria and fungi were observed in the supply air, with more modest reductions in the occupied space. In the case of virus-like particles, there was an eight-fold improvement in equivalent clean air, suggesting a five-fold infection risk reduction for long-range exposure. The data suggest multiple benefits of airborne particle and bioaerosol reduction, with applications beyond healthcare. Long-term studies are recommended to confirm the combined IAQ, health and energy benefits.
Variations in the Appearance and Interpretation of Interpersonal Eye Contact in Social Categorizations and Psychiatric Populations Worldwide: A Scoping Review with a Critical Appraisal of the Literature
Authors: Boer Jos , Boonstra Nynke , Kronenberg Linda , Stekelenburg Ruben , Sizoo Bram
Published: August 2024

Background: Eye contact is one of the most fundamental forms of interhuman communication. However, to date, there has been no comprehensive research comparing how eye contact is made and interpreted in all possible populations worldwide. This study presents a summary of the existing literature on these modalities stratified to social categorizations and psychiatric disorders. Method: A scoping review with critical appraisal of the literature according to the Joanna Briggs Institute (JBI) methodology. Databases AnthroSource, Medline, CINAHL, the Psychology and Behavioral Sciences Collection (EBSCO) and PsychInfo were searched. Results: 7068 articles were screened for both the grey literature and reference lists, of which 385 were included, 282 for social categorizations and 103 for psychiatric disorders. In total, 603 thematic clustered outcomes of variations were included. Methodological quality was generally moderate to good. Conclusions: There is a great degree of variation in the presentation and interpretation of eye contact between and within populations. It remains unclear why specific variations occur in populations. Additionally, no gold standard for how eye contact should be used or interpreted emerged from the studies. Further research into the reason for differences in eye contact between and within populations is recommended.
Community-Based Mental Health Challenges and Implications: Examining Factors Influencing Distress and Help-Seeking Behaviors among Korean American Church Leaders and Members in Greater Los Angeles
Authors: Baek Kelly , Bell Christi , Montgomery Susanne , Ortiz Larry , Kumar Akinchita , Alemi Qais
Published: August 2024

There is limited research on the factors that impact mental distress among Korean American (KA) church leaders even though their unique social situation can create many barriers to seeking mental health assistance. This study compared factors impacting mental distress and help-seeking behaviors between KA church leaders (CLs) and church members (CMs) in the greater Los Angeles area. The respondents (N = 243) were mostly female, married, educated, first-generation immigrants with a mean age of 47.9 years (SD = 19.7). The Hopkins Symptoms Checklist 10 was used to measure anxiety and depression. Hierarchal linear regressions showed that health status exerted the strongest effect on both anxiety and depression among CLs and CMs. Beyond health status, education (only for depression), informal resource use, and resiliency impacted mental distress scores for CLs. Only resiliency and religious coping predicted depression scores among CMs. To effectively reach this population, community-based organizations and behavioral health specialists should consider collaborating with churches to promote and provide essential mental health support. Our findings also highlight that the needs of church leaders (CLs) and church members (CMs) differ, which should guide the development of culturally tailored interventions that build on the resilience of both groups.
Association between Heat Vulnerability Index and Stroke Severity
Authors: Wang Jason J. , Katz Jeffrey M. , Sanmartin Maria X. , Sinvani Liron D. , Naidich Jason J. , Rula Elizabeth Y. , Sanelli Pina C.
Published: August 2024

Background: Socioeconomically disadvantaged neighborhoods are particularly vulnerable to heat-related illnesses. We aim to investigate the association between the heat vulnerability index (HVI), an established neighborhood-level metric of heat-related mortality risk, and acute ischemic stroke (AIS) severity. Methods: We conducted a retrospective analysis of consecutive AIS admissions to a comprehensive stroke center between 2012 and 2021. Stroke severity was defined upon admission based on the National Institutes of Health Stroke Scale (NIHSS). Demographic, socioeconomic, and clinical characteristics were extracted from electronic health records. HVI status was assigned using residential ZIP codes. Multivariable logistic regression analyses were performed. Results: Of 3429 AIS admissions, 1123 (32.8%) were from high-HVI (scores 4–5) neighborhoods and 868 (25.3%) had severe stroke (NIHSS score ≥ 10). In the multivariable regression model with stepwise selection, a high HVI was independently associated with severe stroke (adjusted odds ratio: 1.40 [95% confidence interval 1.16–1.69]). Conclusions: The association between a high HVI and severe stroke underscores the importance of targeting policy interventions to mitigate heat-related illness in socioeconomically disadvantaged neighborhoods.
Social–Emotional Profiles of Preschool Children: An Investigation of Demographic Disparities and Intersectionality
Authors: Chen Chin-Chih , Xu Yaoying , LoCasale-Crouch Jennifer , Xia Yuyan , Rudasill Kathleen , Xie Lindai , Johansen Karli , Joy Jeen , Askue-Collins Jennifer
Published: August 2024

This study aims to enhance our understanding of the diverse nature of social–emotional development and explore the demographic disparities and intersectionality of social determinants among children, with an emphasis on underserved populations of children in low-resource environments. Young children living in low-income families are exposed to a wide array of social and systemic risks that increase the propensity for poor learning and social–emotional development. Using data from the Head Start Family and Childhood Experiences Survey (FACES, this study focuses on the social–emotional development of a nationally representative sample of young children enrolled in the Head Start program (n = 1921, 50.18% male). Employing a person-centered approach, we assessed teacher-rated social–emotional competence, including approach to learning, social cooperation, aggression, hyperactivity, and anxiety/depression/withdrawal, to classify young children’s social–emotional development. This study identified four distinct social–emotional profiles—Adaptive, Average, Moderate Risk, and High Risk—through latent profile analysis. Furthermore, multinomial regression analysis revealed demographic disparities within each social–emotional profile, and significant intersectionality was found between race/ethnicity, age, and disability status in the social–emotional profiles. This research provides valuable insights for better supporting each child’s unique needs.
Sepsis Biomarkers: Advancements and Clinical Applications—A Narrative Review
Authors: He Rong-Rong , Yue Guo-Li , Dong Mei-Ling , Wang Jia-Qi , Cheng Chen
Published: August 2024

Sepsis is now defined as a life-threatening syndrome of organ dysfunction triggered by a dysregulated host response to infection, posing significant challenges in critical care. The main objective of this review is to evaluate the potential of emerging biomarkers for early diagnosis and accurate prognosis in sepsis management, which are pivotal for enhancing patient outcomes. Despite advances in supportive care, traditional biomarkers like C-reactive protein and procalcitonin have limitations, and recent studies have identified novel biomarkers with increased sensitivity and specificity, including circular RNAs, HOXA distal transcript antisense RNA, microRNA-486-5p, protein C, triiodothyronine, and prokineticin 2. These emerging biomarkers hold promising potential for the early detection and prognostication of sepsis. They play a crucial role not only in diagnosis but also in guiding antibiotic therapy and evaluating treatment effectiveness. The introduction of point-of-care testing technologies has brought about a paradigm shift in biomarker application, enabling swift and real-time patient evaluation. Despite these advancements, challenges persist, notably concerning biomarker variability and the lack of standardized thresholds. This review summarizes the latest advancements in sepsis biomarker research, spotlighting the progress and clinical implications. It emphasizes the significance of multi-biomarker strategies and the feasibility of personalized medicine in sepsis management. Further verification of biomarkers on a large scale and their integration into clinical practice are advocated to maximize their efficacy in future sepsis treatment.
Circular RNA Profile in Atherosclerotic Disease: Regulation during ST-Elevated Myocardial Infarction
Authors: Holme Fredric A. , Huse Camilla , Kong Xiang Yi , Broch Kaspar , Gullestad Lars , Anstensrud Anne Kristine , Andersen Geir Ø. , Amundsen Brage H. , Kleveland Ola , Quiles-Jimenez Ana , Holm Sverre , Aukrust Pål , Alseth Ingrun , Halvorsen Bente , Dahl Tuva B.
Published: August 2024

Circular (circ) RNAs are non-coding RNAs with important functions in the nervous system, cardiovascular system, and cancer. Their role in atherosclerosis and myocardial infarction (MI) remains poorly described. We aim to investigate the potential circRNAs in immune cells during atherogenesis and examine the most regulated during MI and the modulation by interleukin (IL)-6 receptor inhibition by tocilizumab. Wild-type (WT) and ApoE−/− mice were fed an atherogenic diet for 10 weeks, and the circRNA profile was analyzed by circRNA microarray. Whole blood from patients with ST-elevated MI (STEMI) and randomized to tocilizumab (n = 21) or placebo (n = 19) was collected at admission, 3–7 days, and at 6 months, in addition to samples from healthy controls (n = 13). Primers for human circRNA were designed, and circRNA levels were measured using RT-qPCR. mRNA regulation of predicted circRNA targets was investigated by RNA sequencing. The expression of 867 circRNAs differed between atherogenic and WT mice. In STEMI patients, circUBAC2 was significantly lower than in healthy controls. CircANKRD42 and circUBAC2 levels were inversely correlated with troponin T, and for circUBAC2, an inverse correlation was also seen with final infarct size at 6 months. The predicted mRNA targets for circUBAC2 and circANKRD42 were investigated and altered levels of transcripts involved in the regulation of inflammatory/immune cells, apoptosis, and mitochondrial function were found. Finally, tocilizumab induced an up-regulation of circANKRD42 and circUBAC2 3–7 days after percutaneous coronary intervention. CircRNA levels were dysregulated in STEMI, potentially influencing the immune system, apoptosis, and mitochondrial function.
The Impact of Growing Area on the Expression of Fruit Traits Related to Sensory Perception in Two Tomato Cultivars
Authors: D’Esposito Daniela , Di Donato Antimo , Puleo Sharon , Nava Matteo , Diretto Gianfranco , Di Monaco Rossella , Frusciante Luigi , Ercolano Maria Raffaella
Published: August 2024

Environmental conditions greatly influence the quality of tomato fruit by affecting the expression of genes, the abundance of metabolites, and the perception of sensorial attributes. In this study, a fruit transcriptome investigation, a sensory test, and a metabolomic analysis were performed to evaluate the impact of the environment on two popular tomato cultivars grown in two Italian regions. The transcriptional profile of each cultivar, cultivated in two different areas, highlighted differential expression in genes involved in pathways related to cell wall components such as pectin, lignin, and hemicellulose and sugars as well as in amino acids, phenylpropanoids, and pigment synthesis. The cultivation area mainly affects sensory attributes related to texture and flavor and the metabolic pattern of cell wall precursors, sugars, glutamate, aspartate, and carotenoids. In the two genotypes cultivated in the same environment, some attributes and fruit-related quality processes are similarly affected, while others are differently influenced based on the specific genetic makeup of the tomato. A combination of transcriptomic, sensory, and metabolomic data obtained from the two tomato genotypes revealed that the environment has a profound effect on specific sensory traits, providing information on factors that shape the specific characteristics and genetic targets for improving tomato fruit characteristics.
NLRP3 Negative Regulation Mechanisms in the Resting State and Its Implications for Therapeutic Development
Authors: Kim YeJi , Lee Sumin , Park Yong Hwan
Published: August 2024

The NACHT-, leucine-rich-repeat-, and pyrin domain-containing protein 3 (NLRP3) is a critical intracellular sensor of the innate immune system that detects various pathogen- and danger-associated molecular patterns, leading to the assembly of the NLRP3 inflammasome and release of interleukin (IL) 1β and IL-18. However, the abnormal activation of the NLRP3 inflammasome has been implicated in the pathogenesis of autoinflammatory diseases such as cryopyrin-associated autoinflammatory syndromes (CAPS) and common diseases such as Alzheimer’s disease and asthma. Recent studies have revealed that pyrin functions as an indirect sensor, similar to the plant guard system, and is regulated by binding to inhibitory 14-3-3 proteins. Upon activation, pyrin transitions to its active form. NLRP3 is predicted to follow a similar regulatory mechanism and maintain its inactive form in the cage model, as it also acts as an indirect sensor. Additionally, newly developed NLRP3 inhibitors have been found to inhibit NLRP3 activity by stabilizing its inactive form. Most studies and reviews on NLRP3 have focused on the activation of the NLRP3 inflammasome. This review highlights the molecular mechanisms that regulate NLRP3 in its resting state, and discusses how targeting this inhibitory mechanism can lead to novel therapeutic strategies for NLRP3-related diseases.
Disturbance of Immune Microenvironment in Androgenetic Alopecia through Spatial Transcriptomics
Authors: Charoensuksira Sasin , Tantiwong Supasit , Pongklaokam Juthapa , Hanvivattanakul Sirashat , Surinlert Piyaporn , Krajarng Aungkana , Thanasarnaksorn Wilai , Hongeng Suradej , Ponnikorn Saranyoo
Published: August 2024

Androgenetic alopecia (AGA) is characterized by microinflammation and abnormal immune responses, particularly in the upper segment of hair follicles (HFs). However, the precise patterns of immune dysregulation remain unclear, partly due to limitations in current analysis techniques to preserve tissue architecture. The infundibulum, a major part of the upper segment of HFs, is associated with significant clusters of immune cells. In this study, we investigated immune cells around the infundibulum, referred to as peri-infundibular immune infiltration (PII). We employed spatial transcriptome profiling, a high-throughput analysis technology, to investigate the immunological disruptions within the PII region. Our comprehensive analysis included an evaluation of overall immune infiltrates, gene set enrichment analysis (GSEA), cellular deconvolution, differential expression analysis, over-representation analysis, protein-protein interaction (PPI) networks, and upstream regulator analysis to identify cell types and molecular dysregulation in immune cells. Our results demonstrated significant differences in immune signatures between the PII of AGA patients (PII-A) and the PII of control donors (PII-C). Specifically, PII-A exhibited an enrichment of CD4+ helper T cells, distinct immune response patterns, and a bias toward a T helper (Th) 2 response. Immunohistochemistry revealed disruptions in T cell subpopulations, with more CD4+ T cells displaying an elevated Th2 response and a reduced Th1-cytotoxic response compared to PII-C. These findings reveal the unique immune landscapes of PII-A and PII-C, suggesting potential for the development of innovative treatment approaches.
Quercetin Attenuates Acetaldehyde-Induced Cytotoxicity via the Heme Oxygenase-1-Dependent Antioxidant Mechanism in Hepatocytes
Authors: Li Kexin , Kidawara Minori , Chen Qiguang , Munemasa Shintaro , Murata Yoshiyuki , Nakamura Toshiyuki , Nakamura Yoshimasa
Published: August 2024

It is still unclear whether or how quercetin influences the toxic events induced by acetaldehyde in hepatocytes, though quercetin has been reported to mitigate alcohol-induced mouse liver injury. In this study, we evaluated the modulating effect of quercetin on the cytotoxicity induced by acetaldehyde in mouse hepatoma Hepa1c1c7 cells, the frequently used cellular hepatocyte model. The pretreatment with quercetin significantly inhibited the cytotoxicity induced by acetaldehyde. The treatment with quercetin itself had an ability to enhance the total ALDH activity, as well as the ALDH1A1 and ALDH3A1 gene expressions. The acetaldehyde treatment significantly enhanced the intracellular reactive oxygen species (ROS) level, whereas the quercetin pretreatment dose-dependently inhibited it. Accordingly, the treatment with quercetin itself significantly up-regulated the representative intracellular antioxidant-related gene expressions, including heme oxygenase-1 (HO-1), glutamate-cysteine ligase, catalytic subunit (GCLC), and cystine/glutamate exchanger (xCT), that coincided with the enhancement of the total intracellular glutathione (GSH) level. Tin protoporphyrin IX (SNPP), a typical HO-1 inhibitor, restored the quercetin-induced reduction in the intracellular ROS level, whereas buthionine sulphoximine, a representative GSH biosynthesis inhibitor, did not. SNPP also cancelled the quercetin-induced cytoprotection against acetaldehyde. These results suggest that the low-molecular-weight antioxidants produced by the HO-1 enzymatic reaction are mainly attributable to quercetin-induced cytoprotection.
Interplay between Multisystem Inflammatory Syndrome in Children, Interleukin 6, Microbiome, and Gut Barrier Integrity
Authors: Zari Ali , Redwan Elrashdy M. , Raszek Mikolaj , Cowley David , Hromić-Jahjefendić Altijana , Uversky Vladimir N. , Fabrowski Mark , Brogna Carlo , Piscopo Marina , Rubio-Casillas Alberto
Published: August 2024

A severe consequence of SARS-CoV-2 infection that manifests as systemic inflammation and multi-organ involvement is called Multisystem Inflammatory Syndrome in Children (MIS-C). This review examines the possible relationship between gut barrier integrity, the microbiome, dysregulation of interleukin 6 (IL-6) signaling, and MIS-C. Clinical and biochemical features of MIS-C are comparable to those of other hyper-inflammatory syndromes, suggesting a dysregulated immune response. One possible explanation for the systemic inflammation seen in MIS-C patients is the SARS-CoV-2-induced dysregulation of the IL-6 signaling pathway. In addition, new data suggest a reciprocal link between gut barrier integrity and IL-6. SARS-CoV-2 exhibits bacteriophage-like behavior, highlighting the role of bacteria as a reservoir for the virus and emphasizing the importance of understanding the bacteriophagic mechanism of the virus in fecal–oral transmission. The increased translocation of viral products and bacterial toxins may result from disrupting the intestinal barrier and cause systemic inflammation. On the other hand, systemic inflammation can weaken the integrity of the intestinal barrier, which feeds back into the loop of immunological dysregulation. In the context of MIS-C, understanding the interaction between SARS-CoV-2 infection, IL-6, and gut barrier integrity may shed light on the etiology of the disease and guide treatment options. Since children with gut dysbiosis may be more susceptible to MIS-C, it is critical to reinforce their microbiome through probiotics supplementation, and plant-fiber-rich diets (prebiotics). Early antibiotic treatment and the use of zonulin antagonists should also be considered.
From Data to Insight: Transforming Online Job Postings into Labor-Market Intelligence
Authors: Tzimas Giannis , Zotos Nikos , Mourelatos Evangelos , Giotopoulos Konstantinos C. , Zervas Panagiotis
Published: August 2024

In the continuously changing labor market, understanding the dynamics of online job postings is crucial for economic and workforce development. With the increasing reliance on Online Job Portals, analyzing online job postings has become an essential tool for capturing real-time labor-market trends. This paper presents a comprehensive methodology for processing online job postings to generate labor-market intelligence. The proposed methodology encompasses data source selection, data extraction, cleansing, normalization, and deduplication procedures. The final step involves information extraction based on employer industry, occupation, workplace, skills, and required experience. We address the key challenges that emerge at each step and discuss how they can be resolved. Our methodology is applied to two use cases: the first focuses on the analysis of the Greek labor market in the tourism industry during the COVID-19 pandemic, revealing shifts in job demands, skill requirements, and employment types. In the second use case, a data-driven ontology is employed to extract skills from job postings using machine learning. The findings highlight that the proposed methodology, utilizing NLP and machine-learning techniques instead of LLMs, can be applied to different labor market-analysis use cases and offer valuable insights for businesses, job seekers, and policymakers.
Minimum Mean Squared Error Estimation and Mutual Information Gain
Authors: Gibson Jerry
Published: August 2024

Information theoretic quantities such as entropy, entropy rate, information gain, and relative entropy are often used to understand the performance of intelligent agents in learning applications. Mean squared error has not played a role in these analyses, primarily because it is not felt to be a viable performance indicator in these scenarios. We build on a new quantity, the log ratio of entropy powers, to establish that minimum mean squared error (MMSE) estimation, prediction, and smoothing are directly connected to mutual information gain or loss in an agent learning system modeled by a Markov chain for many probability distributions of interest. Expressions for mutual information gain or loss are developed for MMSE estimation, prediction, and smoothing, and an example for fixed lag smoothing is presented.
Exploring Tourist Experience through Online Reviews Using Aspect-Based Sentiment Analysis with Zero-Shot Learning for Hospitality Service Enhancement
Authors: Nawawi Ibrahim , Ilmawan Kurnia Fahmy , Maarif Muhammad Rifqi , Syafrudin Muhammad
Published: August 2024

Hospitality services play a crucial role in shaping tourist satisfaction and revisiting intention toward destinations. Traditional feedback methods like surveys often fail to capture the nuanced and real-time experiences of tourists. Digital platforms such as TripAdvisor, Yelp, and Google Reviews provide a rich source of user-generated content, but the sheer volume of reviews makes manual analysis impractical. This study proposes integrating aspect-based sentiment analysis with zero-shot learning to analyze online tourist reviews effectively without requiring extensive annotated datasets. Using pretrained models like RoBERTa, the research framework involves keyword extraction, sentence segment detection, aspect construction, and sentiment polarity measurement. The dataset, sourced from TripAdvisor reviews of attractions, hotels, and restaurants in Central Java, Indonesia, underwent preprocessing to ensure suitability for analysis. The results highlight the importance of aspects such as food, accommodation, and cultural experiences in tourist satisfaction. The findings indicate a need for continuous service improvement to meet evolving tourist expectations, demonstrating the potential of advanced natural language processing techniques in enhancing hospitality services and customer satisfaction.
Molecular Phylogeny of the Family Cordulegastridae (Odonata) Worldwide
Authors: Schneider Thomas , Vierstraete Andy , Kosterin Oleg E. , Ikemeyer Dietmar , Hu Fang-Shuo , Novelo-Gutiérrez Rodolfo , Kompier Tom , Everett Larry , Müller Ole , Dumont Henri J.
Published: August 2024

In this study, we present the first attempt at a molecular phylogenetic analysis of the entire family of Cordulegastridae involving 60% of its known species. Our analysis is in favor of reclassification of the members of the family into four genera: (i) the monophyletic genus Anotogaster Selys, 1854, with the number of known species reduced by three synonymizations; (ii) the genus Cordulegaster Leach in Brewster, 1815 including all members of the boltonii group and, as a preliminary solution, the American species C. virginiae Novelo-Gutiérrez, 2018 and, very tentatively, C. diadema Selys, 1868. The bidentata group forms a genus of its own, for which we restored the name Thecagaster Selys 1854, stat. rev. Cordulegaster pekinensis McLachlan in Selys, 1886, currently considered as Neallogaster pekinensis, was placed by us in Thecagaster as well. The genus Neallogaster Cowley, 1934 needs further investigation involving all remaining species listed in it. The genus Zoraena Kirby, 1890, stat. rev., was recovered to accommodate the remaining American species of Cordulegaster. We synonymized three species of Anotogaster: Anotogaster gregoryi Fraser, 1923 = Anotogaster xanthoptera Lohmann, 1993, syn. nov.; Anotogasterkuchenbeiseri (Förster, 1899) = Anotogaster antehumeralis Lohmann, 1993, syn. nov.; Anotogaster kuchenbeiseri (Förster, 1899) = Anotogaster cornutifrons Lohmann, 1993, syn. nov., based on examination of the existing type specimens. The type of specimens of A. klossi Fraser, 1919 = A. flaveola Lohmann 1993 syn. confirm., were also examined, and their synonymy was confirmed. The isolated populations of A. sieboldii (Selys, 1854) from the archipelagos of Okinawa and Amami Oshima in Japan, respectively, should be regarded as separate species, which will be described elsewhere. Furthermore, we suggest the synonymization of Cordulegaster parvistigma Selys 1873 syn. nov. with Thecagaster brevistigma (Selys 1854) comb. restaur.
Revision of the Genus Laelius (Hymenoptera, Chrysidoidea, Bethylidae) from China
Authors: Wang Chunhong , He Junhua , Chen Xuexin
Published: August 2024

The genus Laelius from China is revised for the first time and six species are recognized, including one new species as well as three new records. The new species, Laelius longus sp. nov., which is supported by both morphological and molecular analyses, is described and illustrated. Three new records, L. naniwaensis, L. nigrofemoratus, and L. yamatonis, are illustrated. A key to the Chinese species of Laelius is provided.
Surgical Management of Complicated Abdominal Tuberculosis: The First Systematic Review—New Treatments for an Ancient Disease and the State of the Art
Authors: Di Buono Giuseppe , Romano Giorgio , Amato Giuseppe , Barletta Gabriele , Romano Giorgio , Adelfio Nicoletta , Geraci Girolamo , Agrusa Antonino
Published: August 2024

Background: Abdominal tuberculosis comprises all forms of tuberculosis that involve the gastrointestinal tract. Controversies exist regarding the surgical approach and timing and type of intervention for complicated forms of abdominal tuberculosis. The aim of this systematic review is to define the rate of surgical treatment, the type of surgical procedures performed and the role of minimally invasive surgery in the management of abdominal tuberculosis. Methods: The literature in MEDLINE, Scopus and Google Scholar and forward and backward citations for studies published between database inception and July 2022 were searched without language restrictions. All prospective and retrospective studies were included. The electronic database search yielded 2440 records. Additionally, eight records were identified through snowball searching. Following duplicate removal (45 duplicates found), 2403 records were screened for titles and abstracts. After screening for titles and abstracts and exclusion criteria, 38 reports were included for systematic review, 27 retrospective studies and 11 prospective studies. Data extracted included the general and demographic characteristics of the studies, diagnostic methods used, clinical presentation, site of involvement and details on surgical treatment. Results: In total, 2870 patients with a diagnosis of abdominal tuberculosis were included, and 1803 (63%) underwent a surgical procedure. The majority of patients underwent an open surgical procedure (95%). The most commonly performed procedures were adhesiolysis (21%) and small bowel resection with primary anastomosis (21%). Conclusions: The results of this review suggest that whenever surgery is required, there is a tendency to perform open surgical procedures in patients with complicated abdominal tuberculosis, both in emergency and elective settings, despite advances in minimally invasive surgery. The study protocol was registered on PROSPERO (CRD42022354322).
Does Indocyanine Green Utilization during Esophagectomy Prevent Anastomotic Leaks? Systematic Review and Meta-Analysis
Authors: Sozzi Andrea , Bona Davide , Yeow Marcus , Habeeb Tamer A. A. M. , Bonitta Gianluca , Manara Michele , Sangiorgio Giuseppe , Biondi Antonio , Bonavina Luigi , Aiolfi Alberto
Published: August 2024

Background: Indocyanine Green (ICG) is a promising technique for the assessment of gastric conduit and anastomosis perfusion during esophagectomy. ICG integration may be helpful in minimizing the risk of anastomotic leak (AL). Literature evidence is sparse, while the real effect of ICG assessment on AL minimization remains unsolved. The aim of this systematic review and meta-analysis was to compare short-term outcomes between ICG-guided and non-ICG-guided (nICG) esophagogastric anastomosis during esophagectomy for cancer. Materials and Methods: PubMed, MEDLINE, Scopus, Web of Science, Cochrane Central Library, and ClinicalTrials.gov were queried up to 25 April 2024. Studies that reported short-term outcomes for ICG versus non-ICG-guided (nICG) anastomosis in patients undergoing esophagectomy were considered. Primary outcome was AL. Risk ratio (RR) and standardized mean difference (SMD) were utilized as effect size measures, whereas to assess relative inference we used 95% confidence intervals (95% CI). Results: Overall, 1399 patients (11 observational studies) were included. Overall, 576 (41.2%) underwent ICG gastric conduit assessment. The patients’ ages ranged from 22 to 91 years, with 73% being male. The cumulative incidence of AL was 10.4% for ICG and 15.4% for nICG. Compared to nICG, ICG utilization was related to a reduced risk for postoperative AL (RR 0.48; 95% CI 0.23–0.99; p = 0.05). No differences were found in terms of pulmonary complications (RR 0.83), operative time (SMD −0.47), hospital length of stay (SMD −0.16), or 90-day mortality (RR 1.70). Conclusions: Our study seems to indicate a potential impact of ICG in reducing post-esophagectomy AL. However, because of limitations in the design of the included studies, allocation/reporting bias, variable definitions of AL, and heterogeneity in ICG use, caution is required to avoid potential overestimation of the ICG effect.
Salzmann Nodular Degeneration in Ocular and Systemic Diseases
Authors: Roszkowska Anna M. , Azzaro Claudia , Calderone Alessandro , Spinella Rosaria , Schiano-Lomoriello Domenico , Mencucci Rita , Wylęgała Adam
Published: August 2024

This review aimed to evidence the predisposing conditions for Salzmann nodular degeneration (SND), where particular attention was paid to its association with ocular and systemic diseases. SND is a rare disease characterized by bluish-white nodules located in the mid-periphery of the cornea, which are otherwise completely clear. SND has been found in association with different systemic and ocular diseases, and it may have unilateral or bilateral presentation. Initial forms are only diagnosed occasionally as they are asymptomatic, whereas, in advanced disease, the visual acuity might be seriously impaired. Although SND is well described, its exact etiopathology is currently still unknown and is frequently misdiagnosed. It is associated with ocular surface inflammatory conditions and previous corneal surgery, and it has been described in different systemic diseases. Diagnosis is clinically based with slit lamp examinations, and instrumental assessments with corneal topography permit one to observe the alterations of the corneal profile, whereas anterior segment–optical coherence tomography (AS-OCT) is used to investigate the stromal depth of the nodules. Therapy might be conservative with the objective of improving the ocular surface homeostasis and surgical outcomes, where the aim is to restore the corneal regularity and visual acuity. Ophthalmologists should pay particular attention when detecting nodules in patients with ocular and non-ocular inflammatory diseases to guarantee the patient a timely diagnosis and a better therapeutic outcome. Additionally, collaboration between specialists who deal with treating patients suffering from disorders potentially associated with SND is recommended.