bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Eye-Tracking to Assess Dyslexia: A Systematic Review of Emerging Evidence
Authors: Toki Eugenia I.
Published: November 2024

Reading is a complex skill that requires accurate word recognition, fluent decoding, and effective comprehension. Children with dyslexia often face challenges in these areas, resulting in ongoing reading difficulties. This study systematically reviews the use of eye-tracking technology to assess dyslexia, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines. The review identifies the specific types of eye-tracking technologies used, examines the cognitive and behavioral abilities assessed (such as reading fluency and attention), and evaluates the primary purposes of these evaluations—screening, assessment, and diagnosis. This study explores key questions, including how eye-tracking outcomes guide intervention strategies and influence educational practices, and assesses the practicality and time efficiency of these evaluations in real-world settings. Furthermore, it considers whether eye-tracking provides a holistic developmental profile or a targeted analysis of specific skills and evaluates the generalizability of eye-tracking results across diverse populations. Gaps in the literature are highlighted, with recommendations proposed to improve eye-tracking’s precision and applicability for early dyslexia intervention. The findings underscore the potential of eye-tracking to enhance diagnostic accuracy through metrics such as fixation counts, saccadic patterns, and processing speed, key indicators that distinguish dyslexic from typical reading behaviors. Additionally, studies show that integrating machine learning with eye-tracking data can enhance classification accuracy, suggesting promising applications for scalable, early dyslexia screening in educational settings. This review provides new insights into the value of eye-tracking technology in identifying dyslexia, emphasizing the need for further research to refine these methods and support their adoption in classrooms and clinics.
Dietary Risk Assessment of Cadmium Exposure Through Commonly Consumed Foodstuffs in Mexico
Authors: Cantoral Alejandra , Collado-López Sonia , Betanzos-Robledo Larissa , Lamadrid-Figueroa Héctor , García-Martínez Betzabeth A. , Ríos Camilo , Díaz-Ruiz Araceli , Mariscal-Moreno Rosa María , Téllez-Rojo Martha María
Published: November 2024

Cadmium (Cd) is a toxic heavy metal widely distributed in foodstuffs. In Mexico, few studies have evaluated Cd content in foods. This study aimed to determine Cd concentrations in foodstuffs that are highly consumed and bought in Mexico City to identify foods exceeding the Maximum Level (ML) and to assess the health risks of theoretical Cd intake from a diet following the Mexican Dietary Guidelines. A total of 143 foodstuffs were analyzed by atomic absorption spectrophotometry. Theoretical Cd intake was estimated in portions per week and compared with the Cd Tolerable Weekly Intake (TWI = 2.5 μg/kg per body weight). A total of 68.5% of the foodstuffs had detectable Cd concentrations. Higher concentrations were found in oyster mushrooms (0.575 mg/kg), romaine lettuce (0.335 mg/kg), and cocoa powder (0.289 mg/kg). Food groups with higher mean concentrations were vegetables (0.084 mg/kg) and snacks, sweets, and desserts (0.049 mg/kg). Ancho chili and romaine lettuce exceed the ML. The theoretical Cd intake estimation was 1.80, 2.05, and 3.82 μg/kg per body weight for adults, adolescents, and school-age children, respectively. This theoretical Cd intake represents a health risk only for school children exceeding the TWI by 53.2%. Our study confirms the presence and risk of Cd in Mexican foodstuffs and highlights the importance of monitoring programs.
Cluster and Principal Component Analyses of the Bioactive Compounds and Antioxidant Activity of Celery (Apium graveolens L.) Under Different Fertilization Schemes
Authors: Milić Anita , Adamović Boris , Nastić Nataša , Tepić Horecki Aleksandra , Pezo Lato , Šumić Zdravko , Pavlić Branimir , Živanov Milorad , Pavković Nemanja , Vojnović Đorđe
Published: November 2024

This research investigates the impact of various fertilization methods on the bioactive compound content and antioxidant activity of celery (Apium graveolens L.) root and leaf. Mineral fertilizer, poultry manure, cattle manure, sheep manure, supercompost, and molasses were applied. Total dry weight, phenolic and flavonoid compounds, and antioxidant activity were assessed, along with fiber, protein, fat, sugar, and starch in celery root. Principal component analysis (PCA) and cluster analysis were used to correlate production conditions with the parameters. The highest fiber and protein contents were found in mineral-fertilized roots, while total fat and sugar were highest in cattle-manure-fertilized roots, and starch was highest in supercompost-fertilized roots. Fertilization with supercompost yielded the highest total phenolic and flavonoid contents in leaves, while mineral fertilizer resulted in the highest antioxidant activity in roots. Notably, the highest dry weight in leaves and the highest total phenolic and flavonoid contents in roots were also observed with supercompost. PCA and cluster analysis demonstrated significant correlations between plant parts, i.e., the celery root and leaf samples, cultivation conditions, and the observed parameters, emphasizing the importance of selecting suitable cultivation methods to optimize celery’s nutritional properties. Also, these findings suggest that supercompost, a byproduct of breweries, could potentially replace animal-based organic fertilizers, addressing the problem of reduced availability due to declining livestock numbers.
Effects of Carbon Dots/PVA Film Combined with Radio Frequency Treatment on Storage Quality of Fried Meatballs
Authors: Zhao Linlin , Jiang Huinan , Han Zhengxuan , Gu Wenqin , Meng Xiangren
Published: November 2024

The combination of carbon dots/polyvinyl alcohol (CDs/PVA) active film and radio frequency (RF) was performed to improve the storage quality of fried meatball samples. The microbicidal effect of RF, and the antioxidant and antibacterial activities of CDs/PVA film were investigated. The CDs/PVA film can effectively restrain the growth of B. subtilis, S. aureus, and E. coli, and eliminate DPPH and ABTS free radicals. RF exerts inhibitory effects on C. albicans, B. subtilis, and E. coli. For meatball samples, CDs/PVA+RF20 can extend the refrigerated shelf life from 2 w to 6 w. At the sixth week, the total bacterial count (TBC) in CDs/PVA+RF20 group (3.72 log CFU/g) was remarkably lower than those in polyethylene terephthalate (PET) group (7.78 log CFU/g) and CDs/PVA (6.41 log CFU/g) group. CDs/PVA+RF20 can also inhibit the increase in TBARS and POV values. The results manifest the feasibility of CDs/PVA+RF as a novel mild pasteurization or preservation technology.
Perceptions and Experiences of Key Informants in Eye Health on the Implementation of Eye Care Health Promotion Interventions in South Africa
Authors: Masemola Hlabje Carel , Baloyi Olivia , Xulu-Kasaba Zamadonda Nokuthula
Published: November 2024

Background: Eye care health promotion interventions aim to encourage the adoption of healthy behaviours that impact eye health and vision impairment, as well as increase the use of eye care services. Thus, this study aims to explore and describe the perceptions of eye care coordinators on the implementation of eye care health promotion interventions in rural Limpopo Province. Methods: This exploratory, descriptive qualitative study employed individual in-depth interviews to collect data from 10 district eye health coordinators. Participants were purposely sampled between June and July 2024. Data were analysed thematically using NVivo version 12. Results: The study revealed seven key themes: human resources in eye health, resource management, policy and governance, eye care services, innovation in eye health, community and patient engagement, and coordination and referral systems. Conclusions: In summary, our study offers important insights into the challenges and opportunities in implementing eye care health promotion interventions. A recommendation is made to address identified challenges such as workforce shortages, inadequate infrastructure, fragmented policies and outdated technologies. Policymakers and eye health professionals can work towards achieving the goals of universal health coverage (UHC) in eye health, ultimately improving eye health outcomes.
Plant-Based Diets in Pediatric Subjects: Heart-Healthy Option or Dangerous Choice?
Authors: Capra Maria Elena , Monopoli Delia , Stanyevic Brigida , Giudice Antonella , Decarolis Nicola Mattia , Esposito Susanna , Biasucci Giacomo
Published: November 2024

Background/Objectives: Plant-based diets (PBDs) are dietary patterns characterized by a certain degree of animal-derived food exclusion. PBDs can be divided into different dietary patterns, from vegetarian to vegan, depending on the degree and the extent of animal-derived food avoidance. PBDs are becoming epidemically popular among the general population, including adult subjects as well as children and adolescents, who often follow the dietary pattern chosen by their families. Methods: Our narrative review aims to analyze the most frequently adopted plant-based dietary patterns in children and adolescents and to evaluate their feasibility, advantages, and risks in terms of health promotion and disease prevention in the developmental age. The MEDLINE–PubMed database was searched to collect and select publications from 1980 to 2024. Results: Subjects following these dietary patterns, especially vegan diets, must be under strict nutritional control and receive adequate micronutrients and vitamin supplementation. Conclusions: Nutrition-skilled professionals should be adequately updated and informed about the feasibility and the risks of these different patterns’ adoption at different ages, as they should guide and accompany children and adolescents and their families in their nutritional choices without prejudices, granting adequate macronutrient and micronutrient intake, adequate growth and neurodevelopment.
The Association Between Clinical Nurses’ Work Environment, Job Stress, and Health Locus of Control and Presenteeism in South Korea
Authors: Park Jin-Young , Eo Yong-Sook
Published: November 2024

Background: This study aimed to examine the relationship between the work environment, job stress, and health locus of control and presenteeism among clinical nurses in South Korea. Methods: A cross-sectional, descriptive correlational study was conducted on clinical nurses (n = 276) from general hospitals in two small cities. Data were collected via a self-administered structured questionnaire from 1 to 14 December 2022. Descriptive and stepwise multiple regression analyses were conducted for this study. Results: The work environment (ß = −0.28, p < 0.001) and education (ß = −0.13, p = 0.031) were significant predictors of health problems, explaining 10% of the variance. Job stress (ß = 0.34, p < 0.001), external locus of control (ß = 0.25, p < 0.001), physician control locus (ß = −0.15, p = 0.006), work department (ß = −0.13, p = 0.018), and marital status (ß = −0.13, p = 0.022) significantly predicted job impairment, accounting for 25% of the variance. For perceived productivity, job stress (ß = −0.18, p = 0.003), marital status (ß = 0.18, p = 0.002), and external locus of control (ß = −0.16, p = 0.007) were influential, explaining 8% of the variance. Conclusions: To mitigate presenteeism among clinical nurses, interventions should focus on improving the work environment at the organizational level and addressing job stress and health locus of control at the individual level. By implementing targeted strategies, healthcare institutions can reduce job impairment and enhance productivity among nursing staff.
Knowledge Gap or Prepared Force? Exploring United Arab Emirates Pharmacy Students and Pharmacists’ Monkeypox Readiness
Authors: Nassar Razan I. , Ahmad Alhareth , Basheti Iman A. , Omar Amin M. , Barqawi Hiba Jawdat , Alzoubi Karem H. , Shahwan Moyad , Al Moukdad AlMuzaffar M. , Al Moukdad Mays Alrim , Abu-Gharbieh Eman
Published: November 2024

Background: The WHO classified the mpox outbreak as a worldwide health emergency. Increasing the contribution of healthcare providers, such as pharmacists, can enhance preventive efforts. Assessing the knowledge and confidence levels of pharmacists in diagnosing and managing mpox cases can shape the response strategies necessary for the management of such outbreaks. Methods: This research employed a cross-sectional survey designed to assess the knowledge and preparedness of pharmacy students and pharmacists in the United Arab Emirates (UAE) regarding the mpox virus outbreak. Independent researchers evaluated the survey items to confirm the face and content validity of the developed survey. The final study’s survey was structured into three distinct sections, each addressing a specific area of interest. Data were analyzed using the IBM SPSS Statistics. Results: The 388 participants had a median age of 22.00 years (IQR = 5.00). The survey revealed that participants primarily relied on the WHO reports for mpox information (79.8%). The total knowledge scores (TK score) varied, ranging from −6 to 23 (median = 6.00), and symptom knowledge scores (SK score) ranged from −3 to 9 (median = 2.00). Older participants (p-value = 0.008) and females (p-value = 0.014) exhibited significantly higher TK scores. Only about 31.0% of participants expressed confidence in diagnosing mpox cases, and 34.6% expressed confidence in managing mpox cases. Nearly a quarter of the participants (24.5%) thought that getting vaccinated against COVID-19 led to contracting mpox more likely, whereas 45.7% believed that a previous infection with COVID-19 increases the risk of having mpox and its associated symptoms. Many respondents (38.7%) expressed their concern that mpox could emerge as the next major epidemic following COVID-19. Conclusion: Although pharmacists and pharmacy students in the UAE are aware of mpox, their knowledge and confidence levels in diagnosing and managing vary significantly. These findings suggest the need for targeted educational programs to enhance the understanding and preparedness of pharmacists to manage and prevent mpox cases.
The Effects of an 8-Week Cognitive–Motor Training Program on Proprioception and Postural Control Under Single and Dual Task in Older Adults: A Randomized Clinical Trial
Authors: Nieto-Guisado Ainhoa , Solana-Tramunt Monica , Cabrejas Cristina , Morales Jose
Published: November 2024

The aim of this study was to assess the effects of an 8-week cognitive-motor training program on postural control and knee proprioception under single and dual task conditions. Design: Randomized clinical trial. Methods: The present study was registered with the ID number NCT04786132. A total of 20 healthy and physically active older adults (73.25 ± 5.98 years) volunteered to participate and were randomly assigned into an experimental and a control group (EG and CG). Postural control was measured with the Romberg test, with open (RBOE) and closed eyes (RBCE) and under unipodal dominant side (RUDL) conditions. Proprioception was assessed by measuring participants’ ability to reposition their dominant knee at 45°. Finally, performance of the cognitive task was measured through a subscale of the Barcelona Test called “categorical evocation in associations”. The EG and the CG completed 8-week training programs with two sessions, 30 min per week, of postural control and proprioception exercises. The EG additionally included music in each session. Results: The analysis using a mixed ANOVA model revealed no significant group × time interaction effects (p < 0.05) for any of the assessments. However, a significant main effect for the time factor was observed, with both the control and experimental groups showing improved outcomes in the post-intervention measurements. Specifically, significant results were found for RBOE (F (4,15) = 11.87, p < 0.001, η2p = 0.76), RBCE (F (4,15) = 11.62, p < 0.001, η2p = 0.75), and proprioception (F (1,18) = 11.53, p < 0.003, η2p = 0.39). Conclusions: The 8-week training program had a positive impact on the post-intervention results for motor control and proprioception, but not on the results of the cognitive task. There were no significant differences between the groups that carried out sessions with or without music.
Validation of the Rosenberg Self-Esteem Scale in Military Personnel of the Spanish Army (RSES-JGA)
Authors: Soriano-Sánchez José Gabriel , Sastre-Riba Sylvia
Published: November 2024

Background: Self-esteem is a psychological term that, since its emergence in 1890 with William James, has been widely studied. Furthermore, this construct has been examined in different areas of psychology, including the clinical field, where its relationship with mental health and low levels of depression has been demonstrated. In the realm of positive psychology, its connection to resilience and life satisfaction has also been established. Evaluating self-esteem in military personnel is essential, as healthy self-esteem can enhance resilience, improve performance, and promote emotional well-being. Objective: To evaluate validity based on internal structure from the perspective of Classical Test Theory (CTT) and Item Response Theory (IRT), to obtain evidence of validity based on the relationship with other variables, and to estimate the reliability of the Rosenberg Self-Esteem Scale (RSES). Method: To this end, 584 military personnel from the three ranks of the Spanish Army (officers, non-commissioned officers, and professional soldiers and sailors) were evaluated, of which 511 were men (87.70%) and 72 were women (12.30%), with an age range of 18 to 66 years (M = 33.17, SD = 7.38). Results: The results indicate that the unidimensional structure of the RSES shows adequate fit indices (CFI = 0.94, TLI = 0.91, SRMR = 0.05), while the difficulty and discrimination parameters exhibited favorable values. Additionally, an acceptable reliability value was found (ɷ = 0.81; α = 0.80). Conclusions: Therefore, it is concluded that the RSES-JGA presents adequate psychometric properties of validity and reliability, making it a useful and relevant instrument for measuring self-esteem in military personnel of the Spanish Army. This will make it possible to measure the level of self-esteem in military personnel, i.e., the set of perceptions, thoughts, evaluations, feelings, and behavioral tendencies directed towards oneself, one’s own way of being, and towards one’s own body and character traits.
Self-Perceived Health, Mood, and Substance Use Among Adolescents: An Analysis to Enhance Family, Community, and Mental Health Care
Authors: Zafra-Agea José Antonio , García-Salido Cristina , Ramírez-Baraldes Estel·la , Vilafranca-Cartagena Mireia , Colillas-Malet Ester , Portabella-Serra Anna , García-Gutiérrez Daniel
Published: November 2024

Background: Adolescence is a critical period for developing self-perception, emotional well-being, and health behaviors. Mental health disorders represent a substantial burden for adolescents worldwide. This study examines self-perceived health, mood, and substance use among adolescents, identifying associated risk factors. Method: A cross-sectional study was conducted with 121 adolescents aged from 14 to 18 from a secondary school in Baix Llobregat, Catalonia. Data were collected through questionnaires, and descriptive and comparative analyses were performed. Results: Poor self-perceived health and negative mood were associated with higher alcohol and tobacco use. Girls exhibited better emotional regulation than boys. Conclusions: Poor health perception and negative mood are linked to increased substance use. Early intervention should focus on emotional well-being and prevention, involving both families and schools.
The Expression and Sequence Analysis of MdMATE52 in Apple Roots During Activation of Defense Against Pythium ultimum Infection
Authors: Zhu Yanmin , Ortiz-Uriarte Bianca , Rainbow Jordan , Zhou Zhe
Published: November 2024

To understand the molecular regulation of host defense responses in the pathosystem between apple roots and a necrotrophic oomycete pathogen Pythium ultimum, a series of transcriptome analyses have revealed a multi-phase and multi-layer defense tactic in apple root tissues. Among the most notable transcriptome changes during defense activation in apple roots, upregulation of genes involved in phenylpropanoid biosynthesis, transport of secondary metabolites, and lignin formation appeared to be the key defense themes which may crucially impact the outcome of plant–pathogen interactions. From our transcriptome datasets, the MdMATE52 gene, which encodes a MATE transporter, was shown to be differentially expressed between a resistant and a susceptible apple rootstock genotype in response to P. ultimum infection. The cis elements at promoter regions and sequence variations within coding regions of MdMATE52 were compared among several resistant and susceptible apple rootstock genotypes as well as various Malus species. The stronger upregulated expression patterns of MdMATE52 appeared to be correlated with the observed resistance traits among various genotypes. Our results suggested that minimal but clearly identifiable sequence variations may contribute to the genotype-specific expression and function of MdMATE52. The findings from this study should facilitate future experiments such as site-specific mutation and Crispr-based genome editing to define the regulation mechanisms of MdMATE52 and function during defense activation in apple roots.
Comprehensive Assessment of the Correlation Between Ancient Tea Garden Soil Chemical Properties and Tea Quality
Authors: Wang Houqiao , Yuan Wenxia , Wang Qiaomei , Xia Yuxin , Chun Wang , Li Haoran , Peng Guochen , Huang Wei , Wang Baijuan
Published: November 2024

Understanding the correlation between soil chemical properties and tea quality is essential for the comprehensive management of ancient tea gardens. However, the specific links between these factors in ancient tea gardens remain underexplored. This study analyzes the soil chemical properties of four distinct research regions in Nanhua County to explore their effects on key chemical components in ancient tea garden teas, providing a scientific basis for improving the quality of ancient tea garden teas through soil management. Employing high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS), the chemical components of tea and the chemical properties of the soil were meticulously quantified. Following these measurements, the integrated fertility index (IFI) and the potential ecological risk index (PERI) were evaluated and correlation analysis was conducted. The results revealed that ancient tea garden tea quality is closely linked to soil chemical properties. Soil’s total nitrogen (TN), total sulfur (TS), and available potassium (AK) negatively correlate with tea’s catechin gallate (CG) component and AK also with polyphenols. Most other soil properties show positive correlations with tea components. The research also evaluated soil heavy metals’ IFI and PERI. IFI varied significantly among regions. Hg’s high pollution index indicates ecological risks; Cd in Xiaochun (XC) region poses a moderate risk. PERI suggests moderate risk for XC and Banpo (BP), with other areas classified as low risk. Implementing reasonable fertilization and soil amelioration measures to enhance soil fertility and ensure adequate supply of key nutrients will improve the quality of ancient tea gardens. At the same time, soil management measures should effectively control heavy metal pollution to ensure the quality and safety of tea products. Insights from this study are crucial for optimizing soil management in ancient tea gardens, potentially improving tea quality and sustainability.
Occurrence of Botrytis cinerea Causing Gray Mold on Pecan in China
Authors: Zheng Xiang-Rong , Huang Xiao-Xiao , Peng Jin-Feng , Gafforov Yusufjon , Chen Jia-Jia
Published: November 2024

Pecan (Carya illinoinensis), a globally economically significant dried fruit and woody oil tree, faces significant challenges in production and nut quality due to the rampant outbreak of severe fungal diseases. From 2020 to 2021, an extensive occurrence of a disease resembling gray mold was observed on the leaves and fruits of pecan trees in Jiangsu Province, China. Upon isolation from symptomatic samples, Botrytis cinerea was identified through morphological analysis and phylogenetic studies of the G3PDH, HSP60, and RPB2 gene sequences. Furthermore, pathogenicity tests conclusively attributed the gray mold disease observed on pecan leaves and fruits to B. cinerea. To the best of our knowledge, this is the first time that B. cinerea has been reported on pecans. These findings thus provide a basis for further research on the management of pecan gray mold.
Morphological Trait Variations and Flower Color Differences in Wild Crocus Species
Authors: Krstić Nataša , Jaćimović Goran , Ljevnaić-Mašić Branka , Petrović Sofija , Prijić Željana , Krstić Đorđe , Banjac Borislav
Published: November 2024

This study investigates the phenotypic diversity of spring-flowering Crocus species native to Serbia by analyzing their morphological traits and flower color variations. Detailed phenotypic characterization was performed on seven species: C. alexandri, C. chrysanthus, C. heuffelianus, C. randjeloviciorum, C. rujanensis, C. variegatus, and C. weldenii. The study examines how morphological parameters of tested species diverged from average values during a hot and dry winter, resulting in smaller plants, earlier and shorter flowering periods, and reduced flower size, regardless of species. Hierarchical clustering based on morphological traits grouped the species into three clusters: the first cluster included C. alexandri, C. chrysanthus, C. rujanensis, and C. weldenii, characterized by the highest number of leaves and the lowest number of flowers; the second consisted of C. randjeloviciorum, distinguished by shorter plant height and the greatest number of flowers; and the third cluster comprised C. heuffelianus and C. variegatus, notable for the tallest plants, widest corms and leaves, and longest perigon segments. Grouping based on petal color components (L*, a*, b*, chroma, and hue) formed five distinct clusters, demonstrating differences in flower color. These findings are valuable for taxonomic classification, conservation measures, and breeding programs, which ultimately support the conservation of biodiversity and the promotion of ornamental plant cultivation. Flower color in particular has proven to be a reliable species indicator, as it remains constant despite the year and/or habitat. The use of a colorimeter can speed up the identification of species in the field and provides researchers and conservationists with a practical tool.
Diversity of Colletotrichum spp. on Aristolochia grandiflora: A Case Study in an Italian Botanical Garden
Authors: Brugneti Federico , Turco Silvia , Rossini Luca , Martignoni Diana , Mazzaglia Angelo
Published: November 2024

During a survey conducted in autumn 2022 and spring 2023, extensive leaf spots were observed on Aristolochia grandiflora plants in the Botanical Garden “Angelo Rambelli” (Viterbo). To preserve the botanical garden collection and avoid disease spread, morphological and molecular identification of the causal agent were carried out. The results revealed three distinct Colletotrichum species belonging to the Colletotrichum boninense and Colletotrichum orchidearum species complexes, which have never been reported together within the same host and, for two of them, never in Italy. These findings may contribute to further extend the state of the art on leaf anthracnose, as well as provide new insights and molecular data for further phylogenetic studies.
Unified Assembly of Chloroplast Genomes: A Comparative Study of Grapes Representing Global Geographic Diversity
Authors: Song Yue , Wang Lujia , Zhang Lipeng , Li Junpeng , Teng Yuanxu , Zhang Zhen , Xu Yuanyuan , Fan Dongying , He Juan , Ma Chao
Published: November 2024

The genus Vitis, known for its economically important fruit—grape—is divided into three geographical groups, American, East Asian, and Eurasian, along with a hybrid group. However, previous studies on grape phylogeny using chloroplast genomes have been hindered by limited sample sizes and inconsistent methodologies, resulting in inaccuracies. In this study, we employed the GetOrganelle software with consistent parameters to assemble the chloroplast genomes of 21 grape cultivars, ensuring comprehensive representation across four distinct groups. A comparative analysis of the 21 grape cultivars revealed structural variation, showing chloroplast genome sizes ranging from 160,813 bp to 161,275 bp. In 21 Vitis cultivars, genome annotation revealed 134 to 136 genes, comprising 89 to 91 protein-coding genes (PCGs), 37 tRNAs, and 8 rRNAs. Our observations have pinpointed specific occurrences of contraction and expansion phenomena at the interfaces between inverted repeat (IR) regions and single-copy (SC) regions, particularly in the vicinity of the rpl2, ycf1, ndhF, and trnN genes. Meanwhile, a total of 193 to 198 SSRs were identified in chloroplast genomes. The diversification pattern of chloroplast genomes exhibited strong concordance with the phylogenetic relationships of the Euvitis subgenera. Phylogenetic analysis based on conserved chloroplast genome strongly clustered the grape varieties according to their geographical origins. In conclusion, these findings enhance our understanding of chloroplast genome variation in Vitis populations and have important implications for cultivar selection, breeding, and conservation efforts.
Identification of the NAC Family and a Functional Analysis of NoNAC36a Under Flooding Stress in Watercress (Nasturtium officinale R.Br.)
Authors: Ding Qiang , Ran Jiajun , Chen Xiaoshan , Gao Zhanyuan , Qian Xiaojun , Zhang Chenyang , Deng Suchong , Shen Yunlou , Wang Yaolong , Li Ying , Hou Xilin
Published: November 2024

Watercress (Nasturtium officinale R.Br.) is a cruciferous aquatic vegetable that possesses significant nutritional value. The NAC family is a transcription factor family specific to plants that play an important role in regulating plant responses to abiotic stress. In order to investigate the response of NAC genes to flooding stress in watercress, we conducted a study on the NoNAC family. In this study, a total of 119 NoNAC genes were obtained through genome-wide identification. Phylogenetic analysis indicated that the NoNAC family members can be categorized into ten subgroups. The results of gene structure analysis revealed that each branch within the subgroups exhibited similar motif composition and gene structure. The heatmap analysis showed that several NoNAC genes demonstrated tissue-specific expression patterns, suggesting their potential as regulators of associated tissue development. As an aquatic plant, watercress serves as a valuable material for investigating plant resistance to flooding stress. This study found that flooding can significantly increase the watercress plant height, which is a typical escape strategy under flooding. The analysis of the expression of NoNAC genes in the stem transcriptome after flooding indicated that only NoNAC36a consistently exhibited significant differential changes and down-regulated expression at the three time points of flooding treatment. This suggests that NoNAC36a may be involved in regulating watercress plant height increases under flooding stress. The utilization of a virus-induced gene silencing assay to investigate the biological function of NoNAC36a revealed that NoNAC36a silencing caused cell elongation and expansion, thus increasing watercress plant height. The yeast one-hybrid and dual luciferase assays demonstrated that NoNAC36a binds the promoter of NoXTH33 and inhibits its expression. Subsequently, the results of yeast two-hybrid, luciferase complementary, and pull-down assays revealed the interaction between NoMOB1A and NoNAC36a in vivo and in vitro. Sequence alignment indicated that NoMOB1A and AtMOB1A share an identical amino acid sequence. RT-qPCR analysis indicated that flooding prompted the expression of NoMOB1A in stems. Thus, it is speculated that NoMOB1A may exhibit functions similar to AtMOB1A and that the up-regulation of NoMOB1A expression in stems may facilitate an increase in plant height under flooding. In summary, the NoNAC family was analyzed, and revealed a regulatory network centered on NoNAC36a that facilitates watercress resistance to flooding stress. This study enhanced the understanding of the NoNAC genes and established a theoretical foundation for investigating plant flooding tolerance.
Predicting Suspended Sediment Transport in Urbanised Streams: A Case Study of Dry Creek, South Australia
Authors: Andualem Tesfa Gebrie , Hewa Guna A. , Myers Baden R. , Boland John , Peters Stefan
Published: November 2024

Sediment transport in urban streams is a critical environmental issue, with significant implications for water quality, ecosystem health, and infrastructure management. Accurately estimating suspended sediment concentration (SSC) is essential for effective long-term environmental management. This study investigates the relationships between streamflow, turbidity, and SSC in Dry Creek, South Australia, to understand sediment transport dynamics in urbanised catchments. We collected grab samples from the field and analysed them in the laboratory. We employed statistical modelling to develop a sediment rating curve (SRC) that provides insights into the sediment transport dynamics in the urban stream. The grab sample measurements showed variations in SSC between 3.2 and 431.8 mg/L, with a median value of 77.3 mg/L. The analysis revealed a strong linear relationship between streamflow and SSC, while turbidity exhibited a two-regime linear relationship, in which the low-turbidity regime demonstrated a stronger linear relationship compared to the high-turbidity regime. This is attributed to the urbanised nature of the catchment, which contributes to a first-flush effect in turbidity. This contributes to sediment hysteresis, resulting in non-proportional turbidity and SSC responses to streamflow changes. The findings demonstrate the effectiveness of a streamflow-based SRC for accurately predicting sediment discharge, explaining 97% of the variability in sediment discharge. The sediment discharge predicted using the SRC indicated a sediment load of 341.8 tonnes per year along the creek. The developed sediment rating curve provides a valuable tool for long-term sediment management in Dry Creek, enabling the assessment of downstream environmental risks. By addressing data limitations, this study contributes to a deeper understanding of sediment transport dynamics in urbanized environments, offering insights for informed decision-making and effective sediment management strategies.
Recent Advances and Prospects in RNA Drug Development
Authors: Tani Hidenori
Published: November 2024

RNA therapeutics have undergone remarkable evolution since their inception in the late 1970s, revolutionizing medicine by offering new possibilities for treating previously intractable diseases. The field encompasses various modalities, including antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs), each with unique mechanisms and applications. The foundation was laid in 1978 with the discovery that synthetic oligonucleotides could inhibit viral replication, followed by pivotal developments such as RNA interference’s discovery in 1998. The COVID-19 pandemic marked a crucial turning point, demonstrating the potential of mRNA vaccines and accelerating interest in RNA-based approaches. However, significant challenges remain, including stability issues, delivery to target tissues, potential off-target effects, and immunogenicity concerns. Recent advancements in chemical modifications, delivery systems, and the integration of AI technologies are addressing these challenges. The field has seen notable successes, such as approved treatments for spinal muscular atrophy and hereditary transthyretin-mediated amyloidosis. Looking ahead, RNA therapeutics show promise for personalized medicine approaches, particularly in treating genetic disorders and cancer. The continued evolution of this field, driven by technological innovations and deeper understanding of RNA biology, suggests a transformative impact on future medical treatments. The purpose of this review is to provide a comprehensive overview of the evolution, current state, and prospects of RNA therapeutics.
Metabolic Dysfunctions, Dysregulation of the Autonomic Nervous System, and Echocardiographic Parameters in Borderline Personality Disorder: A Narrative Review
Authors: Bozzatello Paola , Marin Giacomo , Gabriele Giulio , Brasso Claudio , Rocca Paola , Bellino Silvio
Published: November 2024

Borderline personality disorder (BPD) is a complex psychiatric disorder characterized by an unstable sense of self and identity, emotional dysregulation, impulsivity, and disturbed interpersonal relationships. This narrative review examines the interplay between dysregulation of the autonomic nervous system, metabolic changes, and cardiovascular risk in BPD. Altered heart rate variability (HRV), reflecting the dysregulation of the autonomic nervous system, is associated with some BPD core symptoms, such as emotional instability and impulsivity. Dysregulation of the hypothalamic–pituitary–adrenal (HPA) axis, often stemming from early trauma, contributes to chronic inflammation and elevated allostatic load, which further increases cardiovascular risk. Metabolic dysfunctions in BPD, such as elevated body mass index (BMI), high blood pressure, and inflammatory markers like C-reactive protein (CRP), exacerbate these risks. Speckle-tracking echocardiography, particularly global longitudinal strain (GLS) and biomarkers such as homocysteine and epicardial fat, could be considered early predictors of cardiovascular events in individuals with BPD. Chronic stress, inflammation, and maladaptive stress responses further heighten cardiovascular vulnerability, potentially accelerating biological aging and cognitive decline. A literature search covering the period from 2014 to 2024 on PubMed identified 189 studies on this topic, of which 37 articles were deemed eligible for this review. These included cross-sectional, longitudinal, case–control, randomised controlled trials (RCTs), reviews, and meta-analysis designs, with sample sizes ranging from 14 to 5969 participants. The main limitations were that only one database was searched, the time of publications was limited, non-English manuscripts were excluded, and the quality of each paper was not commented on. This narrative review aims to provide an overview of recent evidence obtained on this topic, pointing out a direction for future research.
Cannabidiol, a Strategy in Aging to Improve Redox State and Immunity in Male Rats
Authors: De la Fuente Mónica , Joyera Noelia , Félix Judith , Díaz-Del Cerro Estefanía , Linillos-Pradillo Beatriz , Rancan Lisa , Tresguerres Jesús A. F.
Published: November 2024

Aging is characterized by oxidative stress and immune function impairment, and is associated with increased morbidity. Cannabidiol (CBD) has anti-oxidant properties, but its role in aging has been scarcely studied. This work aims to test the effect of CBD on the redox state and immunity during aging in rats. In this study, 15-month-old male Long Evans rats received 10 mg/kg b.w/day of CBD in their diet for 10 weeks and were compared with same-age control and 2-month-old rats serving as a young control group, both following a standard diet. After treatment, they were sacrificed, and the spleen, thymus, and total blood cells were collected. Redox parameters such as glutathione reductase and peroxidase activities, reduced (GSH) and oxidized (GSSG) glutathione concentration, GSSG/GSH ratio, and lipid peroxidation were evaluated. Moreover, immune functions (chemotaxis, natural killer activity, and lymphoproliferation) were analyzed in the spleen. Results show that the 15-month-old control rats exhibited increased oxidative stress and immunosenescence compared to the 2-month-old rats. However, the CBD-treated animals showed higher anti-oxidant defenses, lower oxidants in the spleen, thymus, and blood cells, and better immunity in the spleen than the corresponding age-matched controls. Therefore, CBD administration neutralizes oxidative stress and improves immunity, suggesting it is a strategy for achieving healthy aging.
Accompanying Hemoglobin Polymerization in Red Blood Cells in Patients with Sickle Cell Disease Using Fluorescence Lifetime Imaging
Authors: Borges da Silva Fernanda Aparecida , Florindo João Batista , Mattos Amilcar Castro de , Costa Fernando Ferreira , Lorand-Metze Irene , Metze Konradin
Published: November 2024

In recent studies, it has been shown that fluorescence lifetime imaging (FLIM) may reveal intracellular structural details in unstained cytological preparations that are not revealed by standard staining procedures. The aim of our investigation was to examine whether FLIM images could reveal areas suggestive of polymerization in red blood cells (RBCs) of sickle cell disease (SCD) patients. We examined label-free blood films using auto-fluorescence FLIM images of 45 SCD patients and compared the results with those of 27 control persons without hematological disease. All control RBCs revealed homogeneous cytoplasm without any foci. Rounded non-sickled RBCs in SCD showed between zero and three small intensively fluorescent dots with higher lifetime values. In sickled RBCs, we found additionally larger irregularly shaped intensively fluorescent areas with increased FLIM values. These areas were interpreted as equivalent to polymerized hemoglobin. The rounded, non-sickled RBCs of SCD patients with homogeneous cytoplasm were not different from those of the erythrocytes of control patients in light microscopy. Yet, variables from the local binary pattern-transformed matrix of the FLIM values per pixel showed significant differences between non-sickled RBCs and those of control cells. In a linear discriminant analysis, using local binary pattern-transformed texture features (mean and entropy) of the erythrocyte cytoplasm of normal appearing cells, the final model could distinguish between SCD patients and control persons with an accuracy of 84.7% of the patients. When the classification was based on the examination of a single rounded erythrocyte, an accuracy of 68.5% was achieved. Employing the Linear Discriminant Analysis classifier method for machine learning, the accuracy was 68.1%. We believe that our study shows that FLIM is able to disclose the topography of the intracellular polymerization process of hemoglobin in sickle cell disease and that the images are compatible with the theory of the two-step nucleation. Furthermore, we think that the presented technique may be an interesting tool for the investigation of therapeutic inhibition of polymerization.
A Two-Step Synthesis of Porous Nitrogen-Doped Graphene for Electrochemical Capacitors
Authors: Li Jiahao , Wu Zhenjia , Huang Rong , Ge Anbang , Ying Jie
Published: November 2024

Porous nitrogen-doped graphene (PNG) materials with high conductivity, high surface area, and chemical stability have displayed superior performance in electrochemical capacitors. However, previously reported methods for fabricating PNG render the processes expensive, hard to control, limited in production, and unsafe as well, thus largely restricting their practical applications. Herein, we present a facile two-step calcination method to prepare PNG using petroleum asphalt as the carbon source to provide the original three-dimensional porous structure directly and using environmentally friendly and high nitrogen content urea as the nitrogen source without adding any etching agent. The porous structure in PNG can largely increase its specific surface area, and the introduction of nitrogen atoms can effectively increase the degree of defects and improve the wettability of PNG. As a result, PNG displays a high specific capacitance of 157 F g−1 at a current density of 1 A g−1 and cycling stability while maintaining 98.68% initial capacitance after 10,000 cycles.
TBCC Domain-Containing Protein Regulates Sporulation and Virulence of Phytophthora capsici via Nutrient-Responsive Signaling
Authors: Guo Yaru , Qiu Xiang , Lai Bingting , Ou Caihuan , Wang Huirong , Guo Hengyuan , Li Linying , Lin Lili , Yu Dan , Liu Wenbo , Norvienyeku Justice
Published: November 2024

Phytopathogenic oomycetes, particularly Phytophthora capsici, the causal agent of Phytophthora blight disease in essential vegetables and fruit crops, remains a persistent challenge in the vegetable production industry. However, the core molecular regulators of the pathophysiology and broad-range host characteristics of P. capsici remain unknown. Here, we used transcriptomics and CRISPR-Cas9 technology to functionally characterize the contributions of a novel gene (PcTBCC1) coding for a hypothetical protein with a tubulin-binding cofactor C domain with a putative chloroplast-targeting peptide (cTP) to the pathophysiological development of P. capsici. We observed significant upregulation in the expression of PcTBCC1 during pathogen–host interactions. However, the vegetative growth of the ∆Pctbcc1 strains was not significantly different from the wild-type strains. PcTBCC1 gene replacement significantly compromised the sporulation, pathogenic differentiation, and virulence of P. capsici. At the same time, ∆Pctbcc1 strains were sensitive to cell wall stress-inducing osmolytes. These observations, coupled with the close evolutionary ties between PcTBCC1 and pathogenic oomycetes and algae, partly support the notion that PcTBCC1 is a conserved determinant of pathogenesis. This study provides insights into the significance of tubulin-binding cofactors in P. capsici and underscores the potential of PcTbcc1 as a durable target for developing anti-oomycides to control phytopathogenic oomycetes.
High p53 Protein Level Is a Negative Prognostic Marker for Pancreatic Adenocarcinoma
Authors: Klein Sebastian M. , Bozko Maria , Toennießen Astrid , Rangno Dennis , Bozko Przemyslaw
Published: November 2024

Pancreatic adenocarcinoma is one of the most aggressive types of cancer. Among different mechanisms generally believed to be important for the development of cancer, aberrant regulation of the p53 protein is a well-known and common feature for many cancer entities. Our work aims to analyze the impact of p53 deregulation and proteins encoded by p53 target genes on the survival of patients suffering from pancreatic adenocarcinoma. We, therefore, focused on the analysis of the selected collective for the TP53 mutation status, the p53 protein level, their correlation, and possible impacts on the prognosis/survival. We compared and analyzed a set of 123 patients. We have extracted information regarding the TP53 mutation status, p53 protein levels, the level of proteins encoded by prominent p53 target genes, and information on the overall survival. Survival analyses were displayed by Kaplan–Meier plots, using the log-rank test, in order to check for statistical significance. Protein levels were compared using the Mann–Whitney Test. We did not find any statistically significant correlation between the TP53 mutation status and the survival of the patients. Moreover, we have not found any significant correlation between the protein amount of prominent p53 target genes and the patients’ survival. However, we see a significant correlation between the p53 protein level in cancer samples and the overall survival of pancreatic adenocarcinoma patients: patients having tumors with a p53 protein level within the upper quartile of all measured cases show a significantly reduced survival compared to the rest of the patients. Thus, in pancreatic adenocarcinoma, the p53 protein level is a relevant marker for prognosis, and cancers having a high p53 protein amount show a shortened patients’ survival. In contrast, for this cancer entity, the TP53 mutation status or the protein amount of prominent p53 target genes on their own seems not to have a significant impact on survival.