bluuebunny/crossref_metadata_2025 huggingface dataset:
Analyzing Spatio-Temporal Factors to Estimate the Response Time between SMOS and In-Situ Soil Moisture at Different Depths
Authors: Herbert Christoph , Pablos Miriam , Vall-llossera Mercè , Camps Adriano , Martínez-Fernández José
Published: August 2020

A comprehensive understanding of temporal variability of subsurface soil moisture (SM) is paramount in hydrological and agricultural applications such as rainfed farming and irrigation. Since the SMOS (Soil Moisture and Ocean Salinity) mission was launched in 2009, globally available satellite SM retrievals have been used to investigate SM dynamics, based on the fact that useful information about subsurface SM is contained in their time series. SM along the depth profile is influenced by atmospheric forcing and local SM properties. Until now, subsurface SM was estimated by weighting preceding information of remotely sensed surface SM time series according to an optimized depth-specific characteristic time length. However, especially in regions with extreme SM conditions, the response time is supposed to be seasonally variable and depends on related processes occurring at different timescales. Aim of this study was to quantify the response time by means of the time lag between the trend series of satellite and in-situ SM observations using a Dynamic Time Warping (DTW) technique. DTW was applied to the SMOS satellite SM L4 product at 1 km resolution developed by the Barcelona Expert Center (BEC), and in-situ near-surface and root-zone SM of four representative stations at multiple depths, located in the Soil Moisture Measurements Station Network of the University of Salamanca (REMEDHUS) in Western Spain. DTW was customized to control the rate of accumulation and reduction of time lag during wetting and drying conditions and to consider the onset dates of pronounced precipitation events to increase sensitivity to prominent features of the input series. The temporal variability of climate factors in combination with crop growing seasons were used to indicate prevailing SM-related processes. Hereby, a comparison of long-term precipitation recordings and estimations of potential evapotranspiration (PET) allowed us to estimate SM seasons. The spatial heterogeneity of land use was analyzed by means of high-resolution images of Normalized Difference Vegetation Index (NDVI) from Sentinel-2 to provide information about the level of spatial representativeness of SMOS observations to each in-situ station. Results of the spatio-temporal analysis of the study were then evaluated to understand seasonally and spatially changing patterns in time lag. The time lag evolution describes a variable characteristic time length by considering the relevant processes which link SMOS and in-situ SM observation, which is an important step to accurately infer subsurface SM from satellite time series. At a further stage, the approach needs to be applied to different SM networks to understand the seasonal, climate- and site-specific characteristic behaviour of time lag and to decide, whether general conclusions can be drawn.
Surface Defects Detection of Stamping and Grinding Flat Parts Based on Machine Vision
Authors: Tian Hongzhi , Wang Dongxing , Lin Jiangang , Chen Qilin , Liu Zhaocai
Published: August 2020

Currently, surface defect detection of stamping grinding flat parts is mainly undertaken through observation by the naked eye. In order to improve the automatic degree of surface defects detection in stamping grinding flat parts, a real-time detection system based on machine vision is designed. Under plane illumination mode, the whole region of the parts is clear and the outline is obvious, but the tiny defects are difficult to find; Under multi-angle illumination mode, the tiny defects of the parts can be highlighted. In view of the above situation, a lighting method combining plane illumination mode with multi-angle illumination mode is designed, and five kinds of defects are automatically detected by different detection methods. Firstly, the parts are located and segmented according to the plane light source image, and the defects are detected according to the gray anomaly. Secondly, according to the surface of the parts reflective characteristics, the influence of the reflection on the image is minimized by adjusting the exposure time of the camera, and the position and direction of the edge line of the gray anomaly region of the multi-angle light source image are used to determine whether the anomaly region is a defect. The experimental results demonstrate that the system has a high detection success rate, which can meet the real-time detection rEquation uirements of a factory.
No Effects of New Zealand Blackcurrant Extract on Physiological and Performance Responses in Trained Male Cyclists Undertaking Repeated Testing across a Week Period
Authors: Montanari Stefano , Şahin Mehmet A. , Lee Ben J. , Blacker Sam D. , Willems Mark E.T.
Published: August 2020

Anthocyanin supplements are receiving attention due to purported benefits to physiological, metabolic, and exercise responses in trained individuals. However, the efficacy of anthocyanin intake over multiple testing days is not known. We compared a placebo and two doses of anthocyanin-rich New Zealand blackcurrant (NZBC) extract (300 and 600 mg·day−1) on plasma lactate, substrate oxidation, and 16.1 km time trial (TT) performance on three occasions over 7-days in a fed state (day 1 (D1), D4, and D7). Thirteen male cyclists participated in a randomized, crossover, placebo-controlled double-blind design. There was no difference in plasma lactate and substrate oxidation between conditions and between days. A time difference was observed between D1 (1701 ± 163 s) and D4 (1682 ± 162 s) for 600 mg (p = 0.05), with an increment in average speed (D1 = 34.3 ± 3.4 vs. D4 = 34.8 ± 3.4 km·h−1, p = 0.04). However, there was no difference between the other days and between conditions. Overall, one week of intake of NZBC extract did not affect physiological and metabolic responses. Intake of 600 mg of NZBC extract showed inconsistent benefits in improving 16.1 km time trial performance over a week period in trained fed cyclists.
Green Roof Design with Engineered Extensive Substrates and Native Species to Evaluate Stormwater Runoff and Plant Establishment in a Neotropical Mountain Climate
Authors: Rey Carlos Vicente , Franco Natalia , Peyre Gwendolyn , Rodríguez Juan Pablo
Published: August 2020

Green roofs are increasingly being implemented in cities for their multiple environmental benefits. Their optimal design requires an appropriate selection of components, including substrates and plant species, to ensure local sustainability in the long term. The present study seeks to assess the runoff quality and quantity of extensive green roofs located in Bogotá (Colombia). The assessment consists of testing different substrates, designed using locally available constituents and a selection of native species. The best performing substrate mixtures, in terms of runoff volume reduction and plant establishment, were jointly evaluated with three native species (i.e., Paepalanthus alpinus, Achryrocline bogotensis and Echeveria ballsii). On average, engineered substrates presented significantly lower concentrations in several water quality parameters (electric conductivity, total phosphorus, phosphates, Total Kjeldahl Nitrogen, nitrates, nitrites, color, biological oxygen demand and chemical oxygen demand) than the commercial extensive substrate used as control. The species Paepalanthus alpinus and Echeveria ballsii showed significant establishment and were considered potentially suitable species for green roofs in Bogotá. The obtained results, therefore, provide recommendations for green roof design in neotropical mountain climate conditions.
A Carbon-Cloth Anode Electroplated with Iron Nanostructure for Microbial Fuel Cell Operated with Real Wastewater
Authors: Sayed Enas Taha , Alawadhi Hussain , Elsaid Khaled , Olabi A. G. , Adel Almakrani Maryam , Bin Tamim Shaikha Tamim , Alafranji Ghada H. M. , Abdelkareem Mohammad Ali
Published: August 2020

Microbial fuel cell (MFC) is an emerging method for extracting energy from wastewater. The power generated from such systems is low due to the sluggish electron transfer from the inside of the biocatalyst to the anode surface. One strategy for enhancing the electron transfer rate is anode modification. In this study, iron nanostructure was synthesized on a carbon cloth (CC) via a simple electroplating technique, and later investigated as a bio-anode in an MFC operated with real wastewater. The performance of an MFC with a nano-layer of iron was compared to that using bare CC. The results demonstrated that the open-circuit voltage increased from 600 mV in the case of bare CC to 800 mV in the case of the iron modified CC, showing a 33% increase in OCV. This increase in OCV can be credited to the decrease in the anode potential from 0.16 V vs. Ag/AgCl in the case of bare CC, to −0.01 V vs. Ag/AgCl in the case of the modified CC. The power output in the case of the modified electrode was 80 mW/m2—two times that of the MFC using the bare CC. Furthermore, the steady-state current in the case of the iron modified carbon cloth was two times that of the bare CC electrode. The improved performance was correlated to the enhanced electron transfer between the microorganisms and the iron-plated surface, along with the increase of the anode surface- as confirmed from the electrochemical impedance spectroscopy and the surface morphology, respectively.
Provision of Public Health Services and Sustainable Development: Evidence for 12 Emerging Countries
Authors: Bajo-Rubio Oscar , Gómez-Plana Antonio G.
Published: August 2020

In this paper, we quantify the effects of an increase in the public provision of health services in a set of 12 emerging economies (i.e., Brazil, Chile, China, Colombia, India, Kazakhstan, Mexico, Morocco, Peru, Russia, South Africa and Tunisia), representing 45% of world population in 2018. We use a computable general equilibrium model and simulate an increase in the real government expenditure devoted to public health services up to a 20% of total government expenditure, which is also assumed to raise labour productivity. This increase leads to expansionary effects in terms of gross domestic product (GDP) and employment for all the economies under analysis and an increase in the ratio of government deficit to GDP, ranging between 3.66 points for Russia and 0.24 points for Colombia. If, in addition, direct tax rates on labour are increased to offset this result, the effects on GDP and employment become contractionary in most cases; whereas if indirect tax rates are those to be increased, small expansionary effects are again the norm with the only exception of Russia.
Recovery of Biomass Fly Ash and HDPE in Innovative Synthetic Lightweight Aggregates for Sustainable Geotechnical Applications
Authors: Porcino Daniela D. , Mauriello Francesco , Bonaccorsi Lucio , Tomasello Giuseppe , Paone Emilia , Malara Angela
Published: August 2020

Sustainable development principles aim to re-utilize wastes to reduce their impact on the environment. In this context, the present contribution shows preliminary results on the preparation of innovative synthetic lightweight aggregates, starting from biomass-derived fly ash and high-density polyethylene (HDPE), to be used in geotechnical applications. The present work focuses on the manufacturing process of aggregate blends (including the selection of the right proportions of the two components) as well as on the relative determination of (i) physical–chemical properties (i.e., chemical composition, morphological analysis, mineral leachability, water absorption, specific gravity, grain size distribution); (ii) permeability features and (iii) mechanical properties (one-dimensional compression and shear strength behavior). The results, gathered from the new synthetic lightweight aggregates and compared with the corresponding ones obtained in a previous study conducted on natural and synthetic lightweight aggregates, appear promising for a potential utilization in geotechnical engineering.
Differences in Technical and Tactical Learning of Football According to the Teaching Methodology: A Study in an Educational Context
Authors: García-Ceberino Juan M. , Gamero María G. , Feu Sebastián , Ibáñez Sergio J.
Published: August 2020

Football performance requires beginning learners to develop both technical skills and tactical awareness. The aim of this study was to examine and contrast the differences in the learning of football across two different teaching methodologies. A total of 35 students, distributed in two class-groups at the fifth-grade level of primary education participated in the study. Each class group participated in just one of the intervention programs (tactical program, n = 17; technical program, n = 18). The Instrument for the Measurement of Learning and Performance in Football was used to evaluate each student’s actions and in relation to specific performance indicators. For each one of the play actions analyzed, the Performance Index of Decision-Making, the Performance Index of Technical Execution, and the Performance Index of Final Results were calculated and these scores were summed to generate the Total Performance Index. The differences in technical and tactical learning between the class-groups were calculated using the Total Performance Index. For this assessment, various statistical tests were used: the Mann–Whitney’s U and the Wilcoxon’s T (for the non-parametric variables) and the T-test for Independent Samples, as well as the T-test for Related Samples (for the parametric variables). Likewise, a 2 × 2 ANOVA was conducted to determine whether the students’ previous experience had an effect on the level of learning. The results indicated improvements with both intervention programs; however, the tactical program provided a higher level of learning than the technical program between the assessment tests. The experience of the students had an effect on the play actions of dribbling the ball and in marking the player without the ball. Physical education teachers are recommended to implement comprehensive methods for technical and tactical football teaching at school.
The BIM2LCA Approach: An Industry Foundation Classes (IFC)-Based Interface to Integrate Life Cycle Assessment in Integral Planning
Authors: Horn Rafael , Ebertshäuser Sebastian , Di Bari Roberta , Jorgji Olivia , Traunspurger René , Both Petra von
Published: August 2020

An increasing degree of digitalization in construction planning offers significant potential for building life cycle assessment (LCA) to reduce access barriers, as well as the assessment effort itself. To realize the widespread application of LCA tools and their potential to effectively minimize life cycle impacts, an open approach is required that allows for flexible application of comprehensive LCA studies and early integration in planning processes. The authors present an approach for LCA integration in all phases of digital planning which aims at a DGNB (Deutsche Gesellschaft für nachhaltiges Bauen) certification based on the open Building Information Modeling (BIM) standard Industry Foundation Classes (IFC). The approach takes into account varying levels of development and resulting data availability during integral planning phases, as well as resulting LCA application contexts. It goes beyond existing strategies and allows one to consider both BIM and LCA software through a workflow based on a single data format. The assessment framework is operationalized through standardized interface development and technical realization following the information delivery manual (IDM) process standardized for IFC interfaces. The Extensible Markup Language (XML) schema, as a specific implementation for certification, provides the target system for LCA data requirements and is generalized to a planning phase specific IDM base table. The technical realization based on respective model view definitions and distributed data suggests a pathway to the standardization of LCA-IFC integration based on an open approach. The overall approach exemplarily applies to the “LERNZENTRUM” at the Karlsruhe Institute of Technology (KIT) campus. We conclude that an open BIM approach for LCA integration in model-based planning is feasible, but requires several adjustments in IFC, LCA, and planning practice. Adding a lifecycle element to the IFC to connect BIM and LCA provides comprehensive feedback for informed decision making based on environmental impact.
Enhancing Rural Innovation and Sustainability Through Impact Assessment: A Review of Methods and Tools
Authors: Lee So , Díaz-Puente José , Vidueira Pablo
Published: August 2020

Assessing impacts in innovation contexts/settings with the aim of fostering sustainability requires tackling complex issues. Literature shows that key sources of this complexity relate to the need to integrate the local context; identify the underlying problems; engage key stakeholders; and reflect on their feedback throughout the innovation process. A systematic literature review on innovation impact assessment reveals that social impacts have been the most studied, thus, where promising methods and tools were used. Nevertheless, there are many unresolved issues beyond assessing social impacts in innovation processes. Literature highlights that building on co-creating innovation processes that respond to stakeholders’ real needs and context, and adapting to changing circumstances by integrating timely feedback from stakeholders are two critical challenges calling for a systems thinking approach. This study proposes Developmental Evaluation (DE) as a systemic approach to evaluation which supports adaptive development in complex environments and that adds value by integrating continuous feedback from diverse stakeholders. As a non-prescriptive evaluation approach in terms of methods and tools, DE can provide meaningful guidance to use diverse methods and tools in furthering ongoing development and adaptation in innovation processes by linking the evaluation activities—impact assessment among them—with the DE principles that are situational, adaptive and continuously responsive.
What Triggers Us to Be Involved in Martial Arts? Relationships between Motivations and Gender, Age and Training Experience
Authors: Malchrowicz-Mośko Ewa , Zarębski Patrycjusz , Kwiatkowski Grzegorz
Published: August 2020

The study aims to recognize the motivations to engage in judo depending on gender, age and level of sports experience among nonelite judokas from the Greater Poland region because previous studies mostly concerned the motivations of top athletes. We supposed that there was a relationship between age, gender, level of sports experience and motivations in judo. During the diagnostic survey, judokas completed the Polish version of the Sport Motivation Scale. In order to determine the motivational profiles of judokas, cluster analysis was performed using the k-means method. The highest values were obtained for motivations related to the intrinsic motivation to experience stimulation and the intrinsic motivation to accomplish, while the lowest values were obtained for motivations related to amotivation and external regulation. The research results showed a relationship between experience in judo training and the motivations of judokas; however, the motivations of the judokas were not differentiated based on their gender or age. In our study, the intrinsic motivation to experience stimulation turned out to be more important for judokas participating in competitions, while recreational athletes more often indicated experiencing amotivation. The intrinsic motivation to accomplish and intrinsic motivation to know turned out to be more important for athletes in training for more than 10 years, while amotivation was more often indicated to be experienced by judokas practicing judo for less than 10 years. Respondents who reported high internal motivation values and had a motivational structure they shaped themselves had been training for longer—for more than 10 years. In our study, the three motivational groups of judokas were identified, and we found that judo experience differentiated belonging to groups. It is recommended to check other martial arts and check more variables in the future, e.g., marital status, which is a potentially important factor in the field of sport motivation.
A Review on Electronic Payments Security
Authors: Hassan Md Arif , Shukur Zarina , Hasan Mohammad Kamrul , Al-Khaleefa Ahmed Salih
Published: August 2020

Modern technology is turning into an essential element in the financial trade. We focus the emphasis of this review on the research on the E-wallet and online payment, which is an element of an electric payment system, to get the pattern of using this service. This research presents a review of 131 research articles published on electronic payment between 2010 and 2020 that uses a qualitative method of answering the research questions (RQ): RQ1: “What are the major security issues regarding using electronic payments”? and RQ2: “What security properties need to comply for secure electronic payments?” With the systematic literature review approach, the results show that interest in E-wallet and online payment has grown significantly during this period, and it was found that for the increasing uses of electronic payments, researchers are more focused on security issues. The results show that, to conquer the key gaps, electronic payment must have some protection properties, namely, availability, authorization, integrity, non-repudiation, authentication, and confidentiality. Nowadays, security problems in electronic payment are usually more demanding than the present security problems on the web. These findings can enable electric transaction providers to strengthen their security methods by boosting their security gaps, as required for relevant services.
Influence of the Installation Position of Submersible Pumps on Deposition Characteristics in Prefabricated Pumping Stations
Authors: Wang Kai , Bao Haifeng , Liu Houlin , Zhang Zixu , Hu Jianbin
Published: August 2020

Based on the discrete phase model (DPM), solid–liquid two-phase flow calculation, particle settling theory and MATLAB image processing technology, the influence of the installation position of submersible pumps on the deposition characteristics in a cylinder-type prefabricated pumping station was studied, and two performance indices, the deposition rate and area ratio of the easy deposition region were proposed. The results show that the area ratio and deposition rate of the easy deposition region increase as the suspension height increases. The discharge effect is best when the suspension height is 0.1H0, however, at this time the inlet bias of the pump is larger, which is not conducive to the smooth operation of submersible pumps. The area ratio and deposition rate of the easy deposition region gradually increase with the increase in the interval of the pumps. As the center distance increases, the area ratio and deposition rate of the easy deposition region basically decreases first and then increases, and reach the minimum at 0.1R and 0R, respectively.
Use of a Refined Corporate Social Responsibility Model to Mitigate Information Asymmetry and Evaluate Performance
Authors: Wang Ya-Lan , Shen Kao-Yi , Huang Jim-Yuh , Luarn Pin
Published: August 2020

While the importance of Corporate Sociable Responsibility (CSR) has been widely acknowledged, research on how to guide a company in evaluating and improving its CSR performance is relatively under-explored. This paper adopts the predominant framework from the United Nations (UN) and proposes a refined CSR model by using a hybrid multiple criteria decision-making (MCDM) approach. The proposed approach is expected to mitigate the potential information asymmetry issue that might deteriorate the CSR performance of a company. To illustrate the hybrid approach, this study analyzes the CSR performance of four publicly listed information technology (IT) manufacturing companies with the participation of senior domain experts, by using the proposed approach. The CSR performance ranking results are consistent by using various experiments, which is similar to the annual CSR contest held by a prominent organization from Taiwan in 2019. In addition, we illustrate how to apply this refined model to gain managerial insights and pursue sustainable CSR improvement with a priority.
On Solutions of the Initial Value Problem for the Three-Term Fractional Differential Equation with Caputo Derivatives
Authors: Kukla Stanisław , Siedlecka Urszula
Published: August 2020

In this paper, two forms of an exact solution and an analytical–numerical solution of the three-term fractional differential equation with the Caputo derivatives are presented. The Prabhakar function and an asymptotic expansion are utilized to present the double series solution. Using properties of the Pochhammer symbol, a solution is obtained in the form of an infinite series of generalized hypergeometric functions. As an alternative for the series solutions of the fractional commensurate equation, a solution received by an analytical–numerical method based on the Laplace transform technique is proposed. This solution is obtained in the form of a finite sum of the Mittag-Leffler type functions. Numerical examples and a discussion are presented.
Trust Perceptions of Metadata in Open-Source Software: The Role of Performance and Reputation
Authors: Alarcon Gene M. , Gibson Anthony M. , Walter Charles , Gamble Rose F. , Ryan Tyler J. , Jessup Sarah A. , Boyd Brian E. , Capiola August
Published: August 2020

Open-source software (OSS) is a key aspect of software creation. However, little is known about programmers’ decisions to trust software from OSS websites. The current study emulated OSS websites and manipulated reputation and performance factors in the stimuli according to the heuristic-systematic processing model. We sampled professional programmers—with a minimum experience of three years—from Amazon Mechanical Turk (N = 38). We used a 3 × 3 within-subjects design to investigate the relationship between OSS reputation and performance on users’ time spent on code, the number of interface clicks, trustworthiness perceptions, and willingness to use OSS code. We found that participants spent more time on and clicked the interface more often for code that was high in reputation. Meta-information included with OSS tools was found to affect the degree to which computer programmers interact with and perceive online code repositories. Furthermore, participants reported higher levels of perceived trustworthiness in and trust toward highly reputable OSS code. Notably, we observed fewer significant main effects for the performance manipulation, which may correspond to participants considering performance attributes mainly within the context of reputation-relevant information. That is, the degree to which programmers investigate and then trust OSS code may depend on the initial reputation ratings.
The Immunotoxicity of Chronic Exposure to High Levels of Lead: An Ex Vivo Investigation
Authors: Pukanha Kawinsaya , Yimthiang Supabhorn , Kwanhian Wiyada
Published: August 2020

Lead (Pb) is a toxic metal known for its wide-ranging adverse health effects. However, a compound of Pb is still used in the caulking process to repair wooden fishing boats. The present study aimed to measure Pb exposure and its immunologic effects in boatyard workers in Nakhon Si Thammarat province, Thailand, in comparison with an age-matched control group of farmers. The age, body mass index, and smoking history in workers (n = 14) and controls (n = 16) did not differ. The median blood Pb concentration was 8.7-fold higher in workers than controls (37.1 versus 4.3 µg/dL, p < 0.001). Workers had 8.4% lower phagocytic active cells than controls (89.9% versus 98.1%, p = 0.019). In response to a mitogen stimulation, the peripheral blood mononuclear cells (PBMCs) from workers produced 2-fold higher ratios of interleukin-4 (IL-4) to interferon-γ than the PBMCs from controls (p = 0.026). Furthermore, Pb-exposed workers had 33.9% lower cytotoxic T (Tc) cells than controls (24.3% versus 36.8%, p = 0.004). In stark contrast, the percentage of regulatory T (Treg) cells in workers was 2.7-fold higher than controls (6.1% versus 2.3%, p < 0.001). In all subjects, blood Pb showed positive correlations with the percentages of Treg cells (r = 0.843, p < 0.001) and IL-4 (r = 0.473, p = 0.041) while showing an inverse correlation with the percentages of Tc cells (r = −0.563, p = 0.015). These findings indicate that chronic high Pb exposure may cause a shift towards humoral immune response, together with a suppression of cellular immunity, thereby suggesting an elevation in cancer risk in Pb-exposed workers.
The Influence of NaCl and Glucose Content on Growth and Ochratoxin A Production by Aspergillus ochraceus, Aspergillus carbonarius and Penicillium nordicum
Authors: Wang Yan , Yan Hao , Neng Jing , Gao Jian , Yang Bolei , Liu Yang
Published: August 2020

Ochratoxin A (OTA) is a nephrotoxic mycotoxin, which deserves particular attention for its widespread contamination of a variety of food and feed. Aspergillus ochraceus, Aspergillus carbonarius, and Penicillium nordicum are an important source of OTA in three different kinds of food commodities, including cereals, grape and dried fruit products, and dry-cured meat products. Deeper knowledge of OTA production and mycelium growth related to the high-sugar or NaCl-rich environments was gained in this manuscript. A. ochraceus and P. nordicum were likely to have greater growth rates in medium supplied with certain concentrations of NaCl (0–80 g/L), and the colony diameter was the largest at the salt content of 40 g/L. P. nordicum was more suitable to grow in NaCl-riched medium, the OTA production was increased to 316 ppb from 77 ppb when 20 g/L NaCl was added. The capability of OTA production was inhibited when salt content was 40 g/L and 60 g/L in A. ochraceus and P. nordicum, respectively. As the glucose content increased to 250 g/L, the capacity of mycelium growth and sporulation was increased significantly in A. ochraceus and A. carbonarius. A. carbonarius was more suitable to grow in high-sugar grape products. OTA production was significantly promoted with an added 100 g/L glucose in A. carbonarius. OTA production was inhibited when glucose content was 150 g/L and in 200 g/L in A. ochraceus and A. carbonarius, respectively. NaCl and glucose have an effect on fungal growth and OTA production, and the activation of biosynthetic genes of OtaA. These results would allow designing new strategies to prevent OTA accumulation on sugar or NaCl-riched foodstuffs and achieve the objective to manufacture cereals, dried vine fruits and dry-cured ham, free of OTA.
Varying Protein Levels Influence Metabolomics and the Gut Microbiome in Healthy Adult Dogs
Authors: Ephraim Eden , Cochrane Chun-Yen , Jewell Dennis E.
Published: August 2020

The optimal ranges of protein for healthy adult dogs are not known. This study evaluated the impact of long-term consumption of foods containing low, medium, and high levels of protein on serum, urine, and fecal metabolites, and gut microbiome in beagles. Following maintenance on a prefeed food for 14 days, dogs (15 neutered males, 15 spayed females, aged 2–9 years, mean initial weight 11.3 kg) consumed the low (18.99%, dry matter basis), medium (25.34%), or high (45.77%) protein foods, each for 90 days, in a William’s Latin Square Design sequence. In serum and/or urine, metabolites associated with inflammation (9,10-dihydroxyoctadecanoic acid (DiHOME)), 12,13-DiHOME) and kidney dysfunction (urea, 5-hydroxyindole sulfate, 7-hydroxyindole sulfate, p-cresol sulfate) increased with higher protein levels in food, while one-carbon pathway metabolites (betaine, dimethylglycine, sarcosine) decreased. Fecal pH increased with protein consumed, and levels of beneficial indoles and short-chain fatty acids decreased while branched-chain fatty acids increased. Beta diversity of the fecal microbiome was significantly different, with increased abundances of proteolytic bacteria with higher protein food. Feeding dogs a high amount of protein leads to a shift to proteolytic gut bacteria, higher fecal pH, and is associated with increased levels of metabolites linked with inflammation and kidney dysfunction.
The Proposed Neurotoxin β-N-Methylamino-l-Alanine (BMAA) Is Taken up through Amino-Acid Transport Systems in the Cyanobacterium Anabaena PCC 7120
Authors: Wang Zi-Qian , Wang Suqin , Zhang Ju-Yuan , Lin Gui-Ming , Gan Nanqin , Song Lirong , Zeng Xiaoli , Zhang Cheng-Cai
Published: August 2020

Produced by cyanobacteria and some plants, BMAA is considered as an important environmental factor in the occurrence of some neurodegenerative diseases. Neither the underlying mechanism of its toxicity, nor its biosynthetic or metabolic pathway in cyanobacteria is understood. Interestingly, BMAA is found to be toxic to some cyanobacteria, making it possible to dissect the mechanism of BMAA metabolism by genetic approaches using these organisms. In this study, we used the cyanobacterium Anabaena PCC 7120 to isolate BMAA-resistant mutants. Following genomic sequencing, several mutations were mapped to two genes involved in amino acids transport, suggesting that BMAA was taken up through amino acid transporters. This conclusion was supported by the protective effect of several amino acids against BMAA toxicity. Furthermore, targeted inactivation of genes encoding different amino acid transport pathways conferred various levels of resistance to BMAA. One mutant inactivating all three major amino acid transport systems could no longer take up BMAA and gained full resistance to BMAA toxicity. Therefore, BMAA is a substrate of amino acid transporters, and cyanobacteria are interesting models for genetic analysis of BMAA transport and metabolism.
Production of Recombinant Gelonin Using an Automated Liquid Chromatography System
Authors: Berstad Maria E. B. , Cheung Lawrence H. , Weyergang Anette
Published: August 2020

Advances in recombinant DNA technology have opened up new possibilities of exploiting toxic proteins for therapeutic purposes. Bringing forth these protein toxins from the bench to the bedside strongly depends on the availability of production methods that are reproducible, scalable and comply with good manufacturing practice (GMP). The type I ribosome-inhibiting protein, gelonin, has great potential as an anticancer drug, but is sequestrated in endosomes and lysosomes. This can be overcome by combination with photochemical internalization (PCI), a method for endosomal drug release. The combination of gelonin-based drugs and PCI represents a tumor-targeted therapy with high precision and efficiency. The aim of this study was to produce recombinant gelonin (rGel) at high purity and quantity using an automated liquid chromatography system. The expression and purification process was documented as highly efficient (4.4 mg gelonin per litre induced culture) and reproducible with minimal loss of target protein (~50% overall yield compared to after initial immobilized metal affinity chromatography (IMAC)). The endotoxin level of 0.05–0.09 EU/mg was compatible with current standards for parenteral drug administration. The automated system provided a consistent output with minimal human intervention and close monitoring of each purification step enabled optimization of both yield and purity of the product. rGel was shown to have equivalent biological activity and cytotoxicity, both with and without PCI-mediated delivery, as rGelref produced without an automated system. This study presents a highly refined and automated manufacturing procedure for recombinant gelonin at a quantity and quality sufficient for preclinical evaluation. The methods established in this report are in compliance with high quality standards and compose a solid platform for preclinical development of gelonin-based drugs.
A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice
Authors: Ke Xianliang , Zhang Yuan , Liu Yan , Miao Yuanjiu , Zheng Caishang , Luo Dan , Sun Jianhong , Hu Qinxue , Xu Yi , Wang Hanzhong , Zheng Zhenhua
Published: August 2020

Enterovirus 71 (EV71) is the major causative pathogen of human hand, foot, and mouth disease (hHFMD) and has evolved to use various cellular receptors for infection. However, the relationship between receptor preference and EV71 virulence has not been fully revealed. By using reverse genetics, we identified that a single E98K mutation in VP1 is responsible for rapid viral replication in vitro. The E98K mutation enhanced binding of EV71-GZCII to cells in a heparan sulfate (HS)-dependent manner, and it attenuated the virulence of EV71-GZCII in BALB/c mice, indicating that the HS-binding property is negatively associated with viral virulence. HS is widely expressed in vascular endothelial cells in different mouse tissues, and weak colocalization of HS with scavenger receptor B2 (SCARB2) was detected. The cGZCII-98K virus bound more efficiently to mouse tissue homogenates, and the cGZCII-98K virus titers in mouse tissues and blood were much lower than the cGZCII virus titers. Together, these findings suggest that the enhanced adsorption of the cGZCII-98K virus, which likely occurs through HS, is unable to support the efficient replication of EV71 in vivo. Our study confirmed the role of HS-binding sites in EV71 infection and highlighted the importance of the HS receptor in EV71 pathogenesis.
Low-Level Ionizing Radiation Induces Selective Killing of HIV-1-Infected Cells with Reversal of Cytokine Induction Using mTOR Inhibitors
Authors: Pinto Daniel O. , DeMarino Catherine , Vo Thy T. , Cowen Maria , Kim Yuriy , Pleet Michelle L. , Barclay Robert A. , Noren Hooten Nicole , Evans Michele K. , Heredia Alonso , Batrakova Elena V. , Iordanskiy Sergey , Kashanchi Fatah
Published: August 2020

HIV-1 infects 39.5 million people worldwide, and cART is effective in preventing viral spread by reducing HIV-1 plasma viral loads to undetectable levels. However, viral reservoirs persist by mechanisms, including the inhibition of autophagy by HIV-1 proteins (i.e., Nef and Tat). HIV-1 reservoirs can be targeted by the “shock and kill” strategy, which utilizes latency-reversing agents (LRAs) to activate latent proviruses and immunotarget the virus-producing cells. Yet, limitations include reduced LRA permeability across anatomical barriers and immune hyper-activation. Ionizing radiation (IR) induces effective viral activation across anatomical barriers. Like other LRAs, IR may cause inflammation and modulate the secretion of extracellular vesicles (EVs). We and others have shown that cells may secrete cytokines and viral proteins in EVs and, therefore, LRAs may contribute to inflammatory EVs. In the present study, we mitigated the effects of IR-induced inflammatory EVs (i.e., TNF-α), through the use of mTOR inhibitors (mTORi; Rapamycin and INK128). Further, mTORi were found to enhance the selective killing of HIV-1-infected myeloid and T-cell reservoirs at the exclusion of uninfected cells, potentially via inhibition of viral transcription/translation and induction of autophagy. Collectively, the proposed regimen using cART, IR, and mTORi presents a novel approach allowing for the targeting of viral reservoirs, prevention of immune hyper-activation, and selectively killing latently infected HIV-1 cells.
How HIV-1 Gag Manipulates Its Host Cell Proteins: A Focus on Interactors of the Nucleocapsid Domain
Authors: Klingler Jéromine , Anton Halina , Réal Eléonore , Zeiger Manon , Moog Christiane , Mély Yves , Boutant Emmanuel
Published: August 2020

The human immunodeficiency virus (HIV-1) polyprotein Gag (Group-specific antigen) plays a central role in controlling the late phase of the viral lifecycle. Considered to be only a scaffolding protein for a long time, the structural protein Gag plays determinate and specific roles in HIV-1 replication. Indeed, via its different domains, Gag orchestrates the specific encapsidation of the genomic RNA, drives the formation of the viral particle by its auto-assembly (multimerization), binds multiple viral proteins, and interacts with a large number of cellular proteins that are needed for its functions from its translation location to the plasma membrane, where newly formed virions are released. Here, we review the interactions between HIV-1 Gag and 66 cellular proteins. Notably, we describe the techniques used to evidence these interactions, the different domains of Gag involved, and the implications of these interactions in the HIV-1 replication cycle. In the final part, we focus on the interactions involving the highly conserved nucleocapsid (NC) domain of Gag and detail the functions of the NC interactants along the viral lifecycle.
Electricity Generation, Salt and Nitrogen Removal and Microbial Community in Aircathode Microbial Desalination Cell for Saline-Alkaline Soil-Washing Water Treatment
Authors: Xu Chenglong , Lu Jialei , Zhao Zhimiao , Zhang Yinjiang , Zhang Jiawei
Published: August 2020

An aircathode microbial desalination cell (AMDC) was successfully started by inoculating anaerobic sludge into the anode of a microbial desalination cell and then used to study the effects of salinity on performance of AMDC and effect of treatment of coastal saline-alkaline soil-washing water. The results showed that the desalination cycle and rate gradually shorten, but salt removal gradually increased when the salinity was decreased, and the highest salt removal was 98.00 ± 0.12% at a salinity of 5 g/L. COD removal efficiency was increased with the extension of operation cycle and largest removal efficiency difference was not significant, but the average coulomb efficiency had significant differences under the condition of each salinity. This indicates that salinity conditions have significant influence on salt removal and coulomb efficiency under the combined action of osmotic pressure, electric field action, running time and microbial activity, etc. On the contrary, COD removal effect has no significant differences under the condition of inoculation of the same substrate in the anode chamber. The salt removal reached 99.13 ± 2.1% when the AMDC experiment ended under the condition of washing water of coastal saline-alkaline soil was inserted in the desalination chamber. Under the action of osmotic pressure, ion migration, nitrification and denitrification, NH4+-N and NO3−-N in the washing water of the desalination chamber were removed, and this indicates that the microbial desalination cell can be used to treatment the washing water of coastal saline-alkaline soil. The microbial community and function of the anode electrode biofilm and desalination chamber were analyzed through high-throughput sequencing, and the power generation characteristics, organics degradation and migration and transformation pathways of nitrogen of the aircathode microbial desalination cell were further explained.
Quantitative Analysis of the Impact of Meteorological Factors on Reference Evapotranspiration Changes in Beijing, 1958–2017
Authors: Liu Wenhui , Zhang Baozhong , Han Songjun
Published: August 2020

The effects of meteorological factors on reference evapotranspiration (ET0) are variable on different time scales, although research tends to focus only on certain time scales. Therefore, using the meteorological data from 1958 to 2017 of Beijing, China, ET0 values over the last 60 years were calculated using Penman–Monteith method. The variation in ET0 values was thus analyzed against four meteorological factors over different time scales. The sensitivity of ET0 to these factors was assessed using a sensitivity coefficient, while the contribution of each factor to ET0 change was quantified by combining this sensitivity coefficient with the factor’s relative change rate over multiple time scales. The results showed that the sensitivity coefficient of relative humidity over different time scales were all negative, while the sensitivity coefficients of net radiation, temperature and wind speed were mostly positive. The main sensitivity factors of ET0 on different time scales varied. On annual time scales, the main factors were relative humidity and temperature. Over annual time scales, relative humidity and net radiation alternated as the main sensitivity factor; while over interannual time scales, the most sensitive factor was relative humidity during 1958–1979 and net radiation thereafter. The contribution of these four meteorological factors to ET0 also fluctuated greatly on intra-annual time scales. On daily time scales, the contributions of temperature and wind speed at the start and end of the year were large, while net radiation and relative humidity were dominant mid-year. On monthly to seasonal time scales, the contributions of these four meteorological factors to ET0 were notable. The contribution of relative humidity was largest in spring and autumn; net radiation was dominant in summer, while temperature and wind speed were dominant in winter. This research on the temporal variability of ET0 response factors is of great significance for understanding regional climate change.
Individual Rainfall Change Based on Observed Hourly Precipitation Records on the Chinese Loess Plateau from 1983 to 2012
Authors: Ding Wenbin , Wang Fei , Jin Kai , Han Jianqiao , Yu Qiang , Ren Qingfu , Shi Shangyu
Published: August 2020

The magnitude and spatiotemporal distribution of precipitation are the main drivers of hydrologic and agricultural processes in soil moisture, runoff generation, soil erosion, vegetation growth and agriculture activities on the Loess Plateau (LP). This study detects the spatiotemporal variations of individual rainfall events during a rainy season (RS) from May to September based on the hourly precipitation data measured at 87 stations on the LP from 1983 to 2012. The incidence and contribution rates were calculated for all classes of rainfall duration and intensity to identify the dominant contribution to the rainfall amount and frequency variations. The trend rates of regional mean annual total rainfall amount (ATR) and annual mean rainfall intensity (ARI) were 0.43 mm/year and 0.002 mm/h/year in the RS for 1983–2012, respectively. However, the regional mean annual total rainfall frequency (ARF) and rainfall events (ATE) were −0.27 h/year and −0.11 times/year, respectively. In terms of spatial patterns, an increase in ATR appeared in most areas except for the southwest, while the ARI increased throughout the study region, with particularly higher values in the northwest and southeast. Areas of decreasing ARF occurred mainly in the northwest and central south of the LP, while ATE was found in most areas except for the northeast. Short-duration (≤6 h) and light rainfall events occurred mostly on the LP, accounting for 69.89% and 72.48% of total rainfall events, respectively. Long-duration (≥7 h) and moderate rainfall events contributed to the total rainfall amount by 70.64% and 66.73% of the total rainfall amount, respectively. Rainfall frequency contributed the most to the variations of rainfall amount for light and moderate rainfall events, while rainfall intensity played an important role in heavy rainfall and rainstorms. The variation in rainfall frequency for moderate rainfall, heavy rainfall, and rainstorms is mainly affected by rainfall duration, while rainfall event was identified as a critical factor for light rainfall. The characteristics in rainfall variations on the Loess Plateau revealed in this study can provide useful information for sustainable water resources management and plans.