bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation and Evaluation of Nanofibrous and Film-Structured Ciprofloxacin Hydrochloride Inserts for Sustained Ocular Delivery: Pharmacokinetic Study in Rabbit’s Eye
Authors: Taghe Shiva , Mirzaeei Shahla , Ahmadi Arian
Published: March 2023

Conventional anti-infective eye drops are the most common forms of drugs prescribed for the management of topical ocular infections. Despite their convenience, topical eye drops face multiple challenges, including limited bioavailability and repetitive administration. The present study aimed to prepare, evaluate, and compare film-structured and nanofibrous ocular inserts using biocompatible polymers of polyvinyl alcohol (PVA) and polycaprolactone (PCL) to achieve sustained ciprofloxacin Hydrochloride (CIP) delivery. The nanofibrous formulations were prepared by electrospinning and glutaraldehyde crosslinking while the film formulation was prepared by solvent casting. Nanofibrous inserts had mean diameters in the range 330–450 nm. Both film and nanofibrous inserts were strong, although the nanofibers had higher flexibility. In vitro antibacterial efficacy against Staphylococcus aureus and Escherichia coli was observed for all formulations and cell viability of more than 70% confirmed their non-toxicity. In vitro release studies showed prolonged release of 2 days for the film and 5 days for the nanofibers compared with a 10-h release of CIP from the eye drop. Pharmacokinetic studies of rabbits’ eyes showed 4.5–5-folds higher AUC for the nanofiber formulations compared with the eye drop. Thus, prolonged-release film-structured and nanofibrous inserts are suitable carriers for ocular delivery of CIP.
Examining the Role of Hypothalamus-Derived Neuromedin-U (NMU) in Bone Remodeling of Rats
Authors: Born-Evers Gabriella , Orr Ashley L. , Hulsey Elizabeth Q. , Squire Maria E. , Hum Julia M. , Plotkin Lilian , Sampson Catherine , Hommel Jonathan , Lowery Jonathan W.
Published: March 2023

Global loss of the neuropeptide Neuromedin-U (NMU) is associated with increased bone formation and high bone mass in male and female mice by twelve weeks of age, suggesting that NMU suppresses osteoblast differentiation and/or activity in vivo. NMU is highly expressed in numerous anatomical locations including the skeleton and the hypothalamus. This raises the possibility that NMU exerts indirect effects on bone remodeling from an extra-skeletal location such as the brain. Thus, in the present study we used microinjection to deliver viruses carrying short-hairpin RNA designed to knockdown Nmu expression in the hypothalamus of 8-week-old male rats and evaluated the effects on bone mass in the peripheral skeleton. Quantitative RT-PCR confirmed approximately 92% knockdown of Nmu in the hypothalamus. However, after six weeks, micro computed tomography on tibiae from Nmu-knockdown rats demonstrated no significant change in trabecular or cortical bone mass as compared to controls. These findings are corroborated by histomorphometric analyses which indicate no differences in osteoblast or osteoclast parameters between controls and Nmu-knockdown samples. Collectively, these data suggest that hypothalamus-derived NMU does not regulate bone remodeling in the postnatal skeleton. Future studies are necessary to delineate the direct versus indirect effects of NMU on bone remodeling.
Endovascular Aneurysm Sac Embolization for Treatment of Ruptured Aneurysms in the Aortoiliac Segment Using N-Butyl-Cyanoacrylate
Authors: Mostafa Karim , Schierenbeck Marie , Trentmann Jens , Gottschalk Hannes , Andersson Julian , Pfarr Julian , Sieren Malte , Jansen Olav , Schäfer Philipp J.
Published: March 2023

Background Aneurysmal rupture in the aortoiliac segment is a severe, life-threatening condition. Nowadays, in addition to surgical treatment, the implantation of a covered stent graft constitutes a feasible, minimally invasive treatment option. A novel approach is the add-on of transarterial aneurysm sac embolization with N-butyl-cyanoacrylate (NBCA). Here, we report our experience of performing this add-on embolization procedure after endovascular aneurysm repair for complex ruptured aneurysms of the aortoiliac segment. Material and Methods We describe six patients (mean age of 75.2 years; all male) with ruptured aneurysms in the visceral aortic and aortoiliac segment in whom a high-volume transarterial aneurysm sac embolization was performed as an add-on therapy to the implantation of an aortic prosthesis. The aim of this add-on intervention was to achieve the definite embolization of the aneurysmal rupture site and to ensure the best possible aneurysmal sealing. We report the feasibility, technical success, and considerations of using NBCA as well as clinical and follow-up imaging results, given their availability. Results Technical success was achieved in all cases. Clinical success was achieved in four cases. No periprocedural complications or reinterventions were reported. The mean full procedure time was 107.8 min. The mean radiation dose was 12,966.1 cGy/cm2. A mean amount of 10.7 mL of NBCA mixed with lipiodol in a 1:3 to 1:5 ratio was used for all patients. Available follow-up imaging up to 36 months after the procedure showed no aneurysm progression or endoleaks. In two patients, the NBCA cast had almost fully dissolved over the course of follow-up. Conclusions Our study underscores the notion that aneurysm sac embolization using high volumes of NBCA with ethiodized oil as an embolic agent is a feasible and add-on treatment option for optimizing the exclusion of the aneurysm from patients with ruptured aneurysms in the aortoiliac segment.
Araeoanasillus leptosomus gen. et sp. nov., (Hemiptera, Cercopoidea, Sinoalidae?), a New Froghopper from Mid-Cretaceous Burmese Amber with Evidence of Its Possible Host Plant
Authors: Poinar George , Brown Alex E.
Published: March 2023

A new genus and species of froghopper, Araeoanasillus leptosomus gen. et sp. nov. (Hemiptera: Cercopoidea, Sinoalidae?), is described from mid-Cretaceous Burmese amber. The new genus possesses the following diagnostic characteristics: slender, medium size body (length, 7.0 mm) with head longer than wide, round eyes; antennae slender with eight antennomeres; pedicel very short, shorter than scape; pronotum with a length/width ratio of 2.4; metatibia with three spines, including one short spine near base and two adjacent, long, thick spines near apex; a single series of 16 thick apical teeth (comb) at metatibial apex; tegmen narrow with a length/width ratio of 3.2; tegmen with coastal area and stigmal cell punctate; CuP meeting base of CuA2; and MP branching at middle of wing. In hind wing, Cu vein forked once. A series of plant trichomes adjacent and attached to the specimen suggests that the froghopper’s host plant was a fern.
Lean and Industry 4.0: A Review of the Relationship, Its Limitations, and the Path Ahead with Industry 5.0
Authors: Moraes André , Carvalho André M. , Sampaio Paulo
Published: March 2023

This article aims to analyze the relationship between Lean and Industry 4.0, further exploring the opportunities for integration with the new concept of Industry 5.0. Departing from a literature review, it shows how the relationship between Industry 4.0 and Lean is—while unanimously positive—clearly orientated towards the more technological aspects. In this scenario, most studies on this relationship highlight the technological side of organizations, emphasizing the integration of Industry 4.0 technology to augment Lean methodologies and tools. As such, most of the apparent value of this relationship derives from the use of technology, and relatively limited inputs input are found on issues related to the human and social factors of organizations—such as leadership, people, integration, and training for new roles and new tasks. In the face of this reality, we evaluate the potential for integration between Lean and Industry 5.0, arguing how Lean may offer a proper perspective to support sustainability, resilience, and human orientation in Industrial contexts.
On the Curvature Properties of “Long” Social Welfare Functions
Authors: Mazzoleni Piera , Pagani Elisa , Perali Federico
Published: March 2023

This study characterizes the concavity properties of the Jorgenson and Slesnick’s social welfare function that is likely the most empirically relevant function among the family of “long” welfare functions. We bridge this knowledge gap using the definition of generalized concavity to show the conditions necessary for the long social welfare function of interest to be decreasing and quasi-convex with respect to prices. Thanks to this result, “long” social welfare functions with regular curvature can be suitable for applied social welfare analysis and policy evaluations.
Branching Random Walks with One Particle Generation Center and Possible Absorption at Every Point
Authors: Filichkina Elena , Yarovaya Elena
Published: March 2023

We consider a new model of a branching random walk on a multidimensional lattice with continuous time and one source of particle reproduction and death, as well as an infinite number of sources in which, in addition to the walk, only the absorption of particles can occur. The asymptotic behavior of the integer moments of both the total number of particles and the number of particles at a lattice point is studied depending on the relationship between the model parameters. In the case of the existence of an isolated positive eigenvalue of the evolution operator of the average number of particles, a limit theorem is obtained on the exponential growth of both the total number of particles and the number of particles at a lattice point.
Combination of Sequential Sampling Technique with GLR Control Charts for Monitoring Linear Profiles Based on the Random Explanatory Variables
Authors: Yeganeh Ali , Parvizi Amineh Mahdi , Shadman Alireza , Shongwe Sandile Charles , Mohasel Seyed Mojtaba
Published: March 2023

Control charts play a beneficial role in the manufacturing process by reduction of non-compatible products and improving the final quality. In line with these aims, several adaptive methods in which samples can be taken with variable sampling rates and intervals have been proposed in the area of statistical process control (SPC). In some SPC applications, it is important to monitor a relationship between the response and independent variables—this is called profile monitoring. This article proposes adaptive generalized likelihood ratio (GLR) control charts based on variable sampling interval (VSI) and sequential sampling (SS) techniques for monitoring simple linear profiles. Because in some real-life problems, it may be possible that the user cannot control the values of explanatory variables; thus, in this paper, we focus on such a scenario. The performance of the proposed method is compared under three different situations, i.e., the fixed sampling rate (FSR), VSI, and SS, based on average time to signal (ATS) criteria for phase II analysis. Since the SS approach uses a novel sampling procedure based on the statistic magnitude, it has a superior performance over other competing charts. Several simulation studies indicate the superiority as the SS approach yields lower ATS values when there are single-step changes in the intercept, slope, standard deviation of the error term, and explanatory variables. In addition, some other related sensitivity analysis indicates that other aspects of the proposed methods, such as computational time, comparison with other control charts, and consideration of fixed explanatory variables. Furthermore, the results are supported by a real-life illustrative example from the adhesive manufacturing industry.
Research on a Dynamic Task Update Assignment Strategy Based on a “Parts to Picker” Picking System
Authors: Liang Kaibo , Zhou Li , Yang Jianglong , Liu Huwei , Li Yakun , Jing Fengmei , Shan Man , Yang Jin
Published: March 2023

Order picking is a crucial operation in the storage industry, with a significant impact on storage efficiency and cost. Responding quickly to customer demands and shortening picking time is crucial given the random nature of order arrival times and quantities. This paper presents a study on the order-picking process in a distribution center, employing a “parts-to-picker” system, based on dynamic order batching and task optimization. Firstly, dynamic arriving orders with uncertain information are transformed into static picking orders with known information. A new method of the hybrid time window is proposed by combining fixed and variable time windows, and an order consolidation batch strategy is established with the aim of minimizing the number of target shelves for picking. A heuristic algorithm is designed to select a shelf selection model, taking into account the constraint condition that the goods on the shelf can meet the demand of the selection list. Subsequently, task division of multi-AGV is carried out on the shelf to be picked, and the matching between the target shelf and the AGVs, as well as the order of the AGVs to complete the task of picking, is determined. A scheduling strategy model is constructed to consider the task completion time as the incorporation of moving time, queuing time, and picking time, with the shortest task completion time as the objective function and AGV task selection as the decision variable. The improved ant colony algorithm is employed to solve the problem. The average response time of the order batching algorithm based on a hybrid time window is 4.87 s, showing an improvement of 22.20% and 40.2% compared to fixed and variable time windows, respectively. The convergence efficiency of the improved ant colony algorithm in AGV task allocation is improved four-fold, with a better convergence effect. By pre-selecting the nearest picking station for the AGVs, the multi-AGV picking system can increase the queuing time. Therefore, optimizing the static picking station selection and dynamically selecting the picking station queue based on the queuing situation are proposed. The Flexsim simulation results show that the queue-waiting and picking completion times are reduced to 34% of the original, thus improving the flexibility of the queuing process and enhancing picking efficiency.
Seven New Lobane Diterpenoids from the Soft Coral Lobophytum catalai
Authors: Zhang Jiarui , Ma Huixue , Jin Shuangshuang , Liu Xuehuan , Li Lei , Liu Zhaonan , Li Guoqiang , Li Pinglin
Published: March 2023

Seven new lobane diterpenoids, namely, lobocatalens A–G (1–7), were isolated from the Xisha soft coral Lobophytum catalai. Their structures, including their absolute configurations, were elucidated via spectroscopic analysis, comparison with the literature data, QM-MNR, and TDDFT-ECD calculations. Among them, lobocatalen A (1) is a new lobane diterpenoid with an unusual ether linkage between C-14 and C-18. In addition, compound 7 showed moderate anti-inflammatory activity in the zebrafish models and cytotoxic activity against the K562 human cancer cell line.
The Blockade of Mitogen-Activated Protein Kinase 14 Activation by Marine Natural Product Crassolide Triggers ICD in Tumor Cells and Stimulates Anti-Tumor Immunity
Authors: Tsai Keng-Chang , Chen Chia-Sheng , Su Jui-Hsin , Lee Yu-Ching , Tseng Yu-Hwei , Wei Wen-Chi
Published: March 2023

Immunogenic cell death (ICD) refers to a type of cell death that stimulates immune responses. It is characterized by the surface exposure of damage-associated molecular patterns (DAMPs), which can facilitate the uptake of antigens by dendritic cells (DCs) and stimulate DC activation, resulting in T cell immunity. The activation of immune responses through ICD has been proposed as a promising approach for cancer immunotherapy. The marine natural product crassolide, a cembranolide isolated from the Formosan soft coral Lobophytum michaelae, has been shown to have cytotoxic effects on cancer cells. In this study, we investigated the effects of crassolide on the induction of ICD, the expression of immune checkpoint molecules and cell adhesion molecules, as well as tumor growth in a murine 4T1 mammary carcinoma model. Immunofluorescence staining for DAMP ectolocalization, Western blotting for protein expression and Z′-LYTE kinase assay for kinase activity were performed. The results showed that crassolide significantly increased ICD and slightly decreased the expression level of CD24 on the surface of murine mammary carcinoma cells. An orthotopic tumor engraftment of 4T1 carcinoma cells indicated that crassolide-treated tumor cell lysates stimulate anti-tumor immunity against tumor growth. Crassolide was also found to be a blocker of mitogen-activated protein kinase 14 activation. This study highlights the immunotherapeutic effects of crassolide on the activation of anticancer immune responses and suggests the potential clinical use of crassolide as a novel treatment for breast cancer.
The Clinical Role of Cardiovascular Magnetic Resonance Imaging in the Assessment of Cardiac Diastolic Dysfunction
Authors: Bhuiya Sabreen , Bhuiya Tanzim , Makaryus Amgad N.
Published: March 2023

Echocardiography is the gold standard clinical tool for the evaluation of left ventricular diastolic dysfunction (LVDD) and is used to validate other cardiac imaging modalities in measuring diastolic dysfunction. We examined Cardiac Magnetic Resonance Imaging (CMR) in detecting diastolic dysfunction using the time-volume curve-derived parameters compared to echocardiographic diastolic parameters. We evaluated patients who underwent both CMR and transthoracic echocardiography (TTE) within 2 ± 1 weeks of each other. On echo, Doppler/Tissue Doppler Imaging (TDI) measurements were obtained. On CMR, peak filling rate (PFR), time to PFR (TPFR), 1/3 filling fraction (1/3FF), and 1/3 filling rate (1/3FR) were calculated from the time-volume curve. Using the commonly employed E/A ratio, 44.4% of patients were found to have LVDD. Using septal E/E′ and lateral E/E′, 29.6% and 48.1% of patients had LVDD, respectively. Correlation was found between left atrial (LA) size and E/A ratio (R = −0.36). Using LVDD criteria for CMR, 63% of patients had diastolic dysfunction. CMR predicted LVDD in 66.7% of the cases. CMR-derived diastolic filling parameters provided a relatively easy and promising method for the assessment of LVDD and can predict the presence of LVDD as assessed by traditional Doppler and TDI methods.
Dimerization of Transmembrane Proteins in Cancer Immunotherapy
Authors: Li Lei , Li Jingying
Published: March 2023

Transmembrane proteins (TMEMs) are integrated membrane proteins that span the entire lipid bilayer and are permanently anchored to it. TMEMs participate in various cellular processes. Some TMEMs usually exist and perform their physiological functions as dimers rather than monomers. TMEM dimerization is associated with various physiological functions, such as the regulation of enzyme activity, signal transduction, and cancer immunotherapy. In this review, we focus on the dimerization of transmembrane proteins in cancer immunotherapy. This review is divided into three parts. First, the structures and functions of several TMEMs related to tumor immunity are introduced. Second, the characteristics and functions of several typical TMEM dimerization processes are analyzed. Finally, the application of the regulation of TMEM dimerization in cancer immunotherapy is introduced.
Application of Turbiscan Stability Index for the Preparation of Alumina Photocatalytic Membranes for Dye Removal
Authors: Blasi Marida , Algieri Catia , Chakraborty Sudip , Calabrò Vincenza
Published: March 2023

In this work, for the first time, the stability of the TiO2 suspensions used for the photocatalytic membrane preparation was studied by considering the Turbiscan Stability Index (TSI). The use of a stable suspension during the membrane preparation (by the dip-coating method) permitted a better dispersion of the TiO2 nanoparticles into the membrane structure due to a reduction of agglomerates formation. The dip-coating was performed on the macroporous structure (external surface) of the Al2O3 membrane to avoid large reduction of the permeability. In addition, the reduction of the suspension infiltration along the membrane’s cross-section allowed us to preserve the separative layer of the modified membrane. The water flux was reduced by about 11% after the dip-coating. The photocatalytic performance of the prepared membranes was evaluated using the methyl orange as a model pollutant. The reusability of the photocatalytic membranes was also demonstrated.
In-Situ Observation of Steel/Slag/Inclusion Interaction by Means of High-Temperature Confocal Scanning Laser Microscopy
Authors: Cejka Julian , Michelic Susanne Katharina
Published: March 2023

Non-metallic inclusions (NMIs) in steels have been the focus of various experimental studies due to their detrimental character. While isolated processes, such as agglomeration or dissolution, have been well investigated, holistic in-situ views with high-temperature confocal scanning laser microscopy (HT-CSLM) have been rare. In this work a novel and suitable method is presented to simultaneously observe agglomeration, movement, detachment, and dissolution of non-metallic inclusions by combined the melting of steel and slag with a dual-crucible approach. Therefore, different steel/slag combinations were tested. It is shown that, with transparent that does not become tarnished by the ions stemming from the steel, the steel’s surface and non-metallic inclusions are observable. Furthermore, technological limitations are discussed, including restrictions regarding the melting point of steels and certain steel/slag combinations.
Effect of Power on Structural and Mechanical Properties of DC Magnetron Sputtered Cr Coatings
Authors: Shi Wanyu , Peng Jian , Xu Zhigang , Shen Qiang , Wang Chuanbin
Published: March 2023

Cr coatings were deposited on the surface of PCrNi1MoA steel by DC magnetron sputtering. The effects of power (100~250 W) on the structure and mechanical properties of the coating were systematically studied. The results show that all Cr coatings have strong (110) preferred orientation and anisotropic surface morphology, and the cross sections are columnar structures. With the increase in power, the deposition rate increases linearly, the surface roughness and grain size increase gradually, and the nanomechanical properties decrease first and then increase slightly. At 100 W, the columnar grain of the coating is compact and continuous, and the wear resistance and plastic deformation resistance are the best, with the highest binding force of 27.04 N. At 200 W, due to grain growth and tensile stress, internal defects increase, and mechanical properties are poor. The combination of coating and substrates is not tight enough, and it is easy to fall off and fail under the action of external force. When the power reaches 250 W, the higher bombardment rate helps the coating release stress, and reduce porosity.
Comparative Assessment of Two Commercial Real-Time PCR Assays for the Diagnosis of Trypanosoma cruzi DNA in Serum
Authors: Kann Simone , Concha Gustavo , Weinreich Felix , Hahn Andreas , Rückert Christian , Kalinowski Jörn , Landt Olfert , Frickmann Hagen
Published: March 2023

This study was performed to comparably assess two commercial real-time PCR assays for the identification of Trypanosoma cruzi DNA in serum. A total of 518 Colombian serum samples with high pre-test probability for infections with either T. cruzi or apathogenic Trypanosoma rangeli were assessed. The assessment comprised the NDO real-time PCR (TIB MOLBIOL, ref. no. 53-0755-96, referred to as the TibMolBiol assay in the following) with specificity for T. cruzi and the RealStar Chagas PCR Kit 1.0 (altona DIAGNOSTICS, order no. 611013, referred to as the RealStar assay in the following) targeting a kinetoplast sequence of both T. cruzi and T. rangeli without further discrimination. To discriminate between T. cruzi- and T. rangeli-specific real-time PCR amplicons, Sanger sequencing results were available for a minority of cases with discordant real-time PCR results, while the amplicons of the remaining discordant samples were subjected to nanopore sequencing. The study assessment indicated a proportion of 18.1% (n = 94) T. cruzi-positive samples next to 24 samples (4.6%) containing DNA of the phylogenetically related but apathogenic parasite T. rangeli. The observed diagnostic accuracy as expressed by sensitivity and specificity was 97.9% (92/94) and 99.3% (421/424) with the TibMolBiol assay and 96.8% (91/94) and 95.0% (403/424) with the RealStar assay, respectively. Reduced specificity resulted from cross-reaction with T. rangeli in all instances (3 cross-reactions with the TibMolBiol assay and 21 cross-reactions with the RealStar assay). DNA from the six discrete typing units (DTUs) of T. cruzi was successfully amplified by both real-time PCR assays. In summary, both assays showed a comparable diagnostic accuracy for the diagnosis of T. cruzi from human serum, with a slightly higher specificity seen for the TibMolBiol assay. The pronounced co-amplification of DNA from apathogenic T. rangeli according to the RealStar assay may be a disadvantage in areas of co-circulation with T. cruzi, while the test performance of the two compared assays will be quite similar in geographic settings where T. rangeli infections are unlikely.
A Bibliometric Analysis on the Research Trend of Exercise and the Gut Microbiome
Authors: Deng Ruiyi , Wang Mopei , Song Yahan , Shi Yanyan
Published: March 2023

This article aims to provide an overview of research hotspots and trends in exercise and the gut microbiome, a field which has recently gained increasing attention. The relevant publications on exercise and the gut microbiome were identified from the Web of Science Core Collection database. The publication types were limited to articles and reviews. VOSviewer 1.6.18 (Centre for Science and Technology Studies, Leiden University, Leiden, the Netherlands) and the R package “bibliometrix” (R Foundation: Vienna, Austria) were used to conduct a bibliometric analysis. A total of 327 eligible publications were eventually identified, including 245 original articles and 82 reviews. A time trend analysis showed that the number of publications rapidly increased after 2014. The leading countries/regions in this field were the USA, China, and Europe. Most of the active institutions were from Europe and the USA. Keyword analysis showed that the relationship between disease, the gut microbiome, and exercise occurs throughout the development of this field of research. The interactions between the gut microbiota, exercise, status of the host’s internal environment, and probiotics, are important facets as well. The research topic evolution presents a trend of multidisciplinary and multi-perspective comprehensive analysis. Exercise might become an effective intervention for disease treatment by regulating the gut microbiome. The innovation of exercise-centered lifestyle intervention therapy may become a significant trend in the future.
A First Insight into the Microbial and Viral Communities of Comau Fjord—A Unique Human-Impacted Ecosystem in Patagonia (42∘ S)
Authors: Guajardo-Leiva Sergio , Mendez Katterinne N. , Meneses Claudio , Díez Beatriz , Castro-Nallar Eduardo
Published: March 2023

While progress has been made in surveying the oceans to understand microbial and viral communities, the coastal ocean and, specifically, estuarine waters, where the effects of anthropogenic activity are greatest, remain partially understudied. The coastal waters of Northern Patagonia are of interest since this region experiences high-density salmon farming as well as other disturbances such as maritime transport of humans and cargo. Here, we hypothesized that viral and microbial communities from the Comau Fjord would be distinct from those collected in global surveys yet would have the distinctive features of microbes from coastal and temperate regions. We further hypothesized that microbial communities will be functionally enriched in antibiotic resistance genes (ARGs) in general and in those related to salmon farming in particular. Here, the analysis of metagenomes and viromes obtained for three surface water sites showed that the structure of the microbial communities was distinct in comparison to global surveys such as the Tara Ocean, though their composition converges with that of cosmopolitan marine microbes belonging to Proteobacteria, Bacteroidetes, and Actinobacteria. Similarly, viral communities were also divergent in structure and composition but matched known viral members from North America and the southern oceans. Microbial communities were functionally enriched in ARGs dominated by beta-lactams and tetracyclines, bacitracin, and the group macrolide–lincosamide–streptogramin (MLS) but were not different from other communities from the South Atlantic, South Pacific, and Southern Oceans. Similarly, viral communities were characterized by exhibiting protein clusters similar to those described globally (Tara Oceans Virome); however, Comau Fjord viromes displayed up to 50% uniqueness in their protein content. Altogether, our results indicate that microbial and viral communities from the Comau Fjord are a reservoir of untapped diversity and that, given the increasing anthropogenic impacts in the region, they warrant further study, specifically regarding resilience and resistance against antimicrobials and hydrocarbons.
Potential of Fungal Endophytes Isolated from Pasture Species in Spanish Dehesas to Produce Enzymes under Salt Conditions
Authors: García-Latorre Carlos , Rodrigo Sara , Santamaría Oscar
Published: March 2023

Endophytic fungi have been found to produce a wide range of extracellular enzymes, which are increasingly in demand for their industrial applications. Different by-products from the agrifood industry could be used as fungal growth substrates for the massive production of these enzymes, specifically as a way to revalorize them. However, such by-products often present unfavorable conditions for the microorganism’s growth, such as high salt concentrations. Therefore, the objective of the present study was to evaluate the potential of eleven endophytic fungi—which were isolated from plants growing in a harsh environment, specifically, from the Spanish dehesas—for the purposes of the in vitro production of six enzymes (i.e., amylase, lipase, protease, cellulase, pectinase and laccase) under both standard and salt-amended conditions. Under standard conditions, the studied endophytes produced between two and four of the six enzymes evaluated. In most of the producer fungal species, this enzymatic activity was relatively maintained when NaCl was added to the medium. Among the isolates evaluated, Sarocladium terricola (E025), Acremonium implicatum (E178), Microdiplodia hawaiiensis (E198), and an unidentified species (E586) were the most suitable candidates for the massive production of enzymes by using growth substrates with saline properties (such as those found in the many by-products from the agrifood industry). This study should be considered an initial approach by which to further study the identification of these compounds as well as to develop the optimization of their production by directly using those residues.
The Nonbacterial Microbiome: Fungal and Viral Contributions to the Preterm Infant Gut in Health and Disease
Authors: Wilson Adam , Bogie Brett , Chaaban Hala , Burge Kathryn
Published: March 2023

The intestinal microbiome is frequently implicated in necrotizing enterocolitis (NEC) pathogenesis. While no particular organism has been associated with NEC development, a general reduction in bacterial diversity and increase in pathobiont abundance has been noted preceding disease onset. However, nearly all evaluations of the preterm infant microbiome focus exclusively on the bacterial constituents, completely ignoring any fungi, protozoa, archaea, and viruses present. The abundance, diversity, and function of these nonbacterial microbes within the preterm intestinal ecosystem are largely unknown. Here, we review findings on the role of fungi and viruses, including bacteriophages, in preterm intestinal development and neonatal intestinal inflammation, with potential roles in NEC pathogenesis yet to be determined. In addition, we highlight the importance of host and environmental influences, interkingdom interactions, and the role of human milk in shaping fungal and viral abundance, diversity, and function within the preterm intestinal ecosystem.
Gochujang Ameliorates Hepatic Inflammation by Improving Dysbiosis of Gut Microbiota in High-Fat Diet-Induced Obese Mice
Authors: Lee Eun-Ji , Edward Olivet Chiamaka , Seo Eun-Bi , Mun Eun-Gyung , Jeong Su-Ji , Ha Gwangsu , Han Anna , Cha Youn-Soo
Published: March 2023

Abnormal fat accumulation with gut microbiota dysbiosis results in hepatic inflammation by upregulating the release of lipopolysaccharide (LPS) and inflammatory cytokine. Gochujang, a traditional fermented condiment, has beneficial effects, such as anti-colonic inflammatory effects. However, Gochujang has been controversial because of its high salt content (the Korean Paradox). Thus, the present study aimed to investigate the preventative effects of Gochujang on hepatic inflammation and related gut microbiota through discussing the Korean Paradox. The mice were divided into groups including a normal diet (ND), high-fat diet (HD), HD with salt (SALT), HD with a high percentage of beneficial microbiota Gochujang (HBM), and HD with diverse beneficial microbiota Gochujang (DBM). Gochujang markedly reduced lipid accumulation, hepatic injury, and inflammation response. Furthermore, Gochujang attenuated protein expression involved in the JNK/IκB/NF-κB pathway. Additionally, Gochujang regulated the gut microbiota-derived LPS production and Firmicutes/Bacteroidetes ratio. Gochujang regulated the levels of gut microbiota such as Bacteroides, Muribaculum, Lactobacillus, and Enterorhabdus, which were correlated with hepatic inflammation. Salt did not have foregoing effects, meaning that the salt content in Gochujang did not affect its anti-inflammatory effect. In conclusion, Gochujang showed anti-hepatic inflammation effects via reduced lipid accumulation, hepatic injury, and inflammatory response together with reorganization of gut microbiota dysbiosis regardless of salt content and the difference of micro bacteria composition.
In Vitro Anti-Venom Potentials of Aqueous Extract and Oils of Toona ciliata M. Roem against Cobra Venom and Chemical Constituents of Oils
Authors: Okot David Fred , Namukobe Jane , Vudriko Patrick , Anywar Godwin , Heydenreich Matthias , Omowumi Oyedeji Adebola , Byamukama Robert
Published: March 2023

There are high mortality and morbidity rates from poisonous snakebites globally. Many medicinal plants are locally used for snakebite treatment in Uganda. This study aimed to determine the in vitro anti-venom activities of aqueous extract and oils of Toona ciliata against Naja melanoleuca venom. A mixture of venom and extract was administered intramuscularly in rats. Anticoagulant, antiphospholipase A2 (PLA2) inhibition assay, and gel electrophoresis for anti-venom activities of oils were done. The chemical constituents of the oils of ciliata were identified using Gas chromatography-tandem mass spectroscopy (GC-MS/MS). The LD50 of the venom was 0.168 ± 0.21 µg/g. The venom and aqueous extract mixture (1.25 µg/g and 3.5 mg/g) did not cause any rat mortality, while the control with venom only (1.25 µg/g) caused death in 1 h. The aqueous extract of T. ciliata inhibited the anticoagulation activity of N. melanoleuca venom from 18.58 min. to 4.83 min and reduced the hemolytic halo diameter from 24 to 22 mm. SDS-PAGE gel electrophoresis showed that oils completely cleared venom proteins. GC-MS/MS analysis showed that the oils had sesquiterpene hydrocarbons (60%) in the volatile oil (VO) and oxygenated sesquiterpenes (48.89%) in the non-volatile oils (NVO). Some major compounds reported for the first time in T. ciliata NVOs were: Rutamarin (52.55%), β-Himachalol (9.53%), Girinimbine (6.68%) and Oprea1 (6.24%). Most compounds in the VO were reported for the first time in T. ciliata, including the major ones Santalene (8.55%) and Himachal-7-ol (6.69%). The result showed that aqueous extract and oils of T. ciliata have anti-venom/procoagulant activities and completely neutralized the venom. We recommend a study on isolation and testing the pure compounds against the same venom.
Sustainable Dielectric Films with Ultralow Permittivity from Soluble Fluorinated Polyimide
Authors: Li Hejian , Kong Xiangyi , Wang Shixiao , Gong Min , Lin Xiang , Zhang Liang , Wang Dongrui
Published: March 2023

In the rapidly growing area of high-frequency communications, polyimide films with ultralow dielectric constant and dielectric loss, adequate insulating strength, and recyclability are in high demand. Using a synthesized soluble fluorinated polyimide, a series of recyclable porous dielectric films with varying porosities were fabricated in this study through nonsolvent-induced phase separation. By manipulating the mass ratio of the binary solvent used to dissolve the polyimide, the shape, size, and size distribution of the pores generated throughout the polyimide matrix can be accurately regulated. The porosity and average pore size of the as-prepared porous films were adjustable between 71% and 33% and between 9.31 and 1.00 μm, respectively, which resulted in a variable dielectric constant of 1.51–2.42 (100 kHz) and electrical breakdown strength of 30.3–119.7 kV/mm. The porous sPI film with a porosity rate of 48% displayed a low dielectric constant of 2.48 at 10 GHz. Coupled with their superior thermal stability, mechanical characteristics, and recyclability, these porous polyimide films are highly promising for constructing high-frequency microelectronic devices.
Structural Insights and Intermolecular Energy for Some Medium and Long-Chain Testosterone Esters
Authors: Turza Alexandru , Pascuta Petru , Mare Liviu , Borodi Gheorghe , Popescu Violeta
Published: March 2023

Testosterone (17β-Hydroxyandrost-4-en-3-one) is the primary male anabolic-androgenic steroid. The crystal structures of two medium and two long esterified forms of testosterone, including enanthate, cypionate, decanoate and undecanoate, were determined by X-ray single crystal diffraction. The samples were also characterized by powder X-ray diffraction, FT-IR spectroscopy and thermal analysis (DTA, TG). Crystal packings and supramolecular features were described. The analysis of structural features was accomplished by computational methods in terms of the type of intermolecular interactions, crystal energies and Hirshfeld surfaces analysis. From a pharmaceutical point of view, the solubility of compounds was investigated.
Structural Characterization, Cytotoxicity, and the Antifungal Mechanism of a Novel Peptide Extracted from Garlic (Allium sativa L.)
Authors: Li Shuqin , Wang Yajie , Zhou Jingna , Wang Jia , Zhang Min , Chen Haixia
Published: March 2023

Garlic (Allium sativa L.) is a traditional plant with antimicrobial activity. This study aimed to discover new antifungal peptides from garlic, identify their structure, and explore the antimicrobial mechanism. Peptides were separated by chromatography and identified by MALDI-TOF analysis. Structure and conformation were characterized by CD spectrum and NMR analysis. Mechanism studies were conducted by SEM, membrane depolarization, and transcriptomic analysis. The cytotoxicity to mammalian cells as well as drug resistance development ability were also evaluated. A novel antifungal peptide named NpRS with nine amino acids (RSLNLLMFR) was obtained. It was a kind of cationic peptide with a α-helix as the dominant conformation. NOESY correlation revealed a cyclization in the molecule. The peptide significantly inhibited the growth of Candida albicans. The mechanism study indicated that membrane destruction and the interference of ribosome-related pathways might be the main mechanisms of antifungal effects. In addition, the resistance gene CDR1 for azole was down-regulated and the drug resistance was hardly developed in 21 days by the serial passage study. The present study identified a novel antifungal garlic peptide with low toxicity and provided new mechanism information for the peptide at the gene expression level to counter drug resistance.
Effect of Oxyfluorination of PFA-Coated Metal Mesh with Superhydrophobic Properties on the Filtration Performance of SiO2 Microparticles
Authors: Kim Kyung-Soo , Kwak Cheol-Hwan , Ha Seong-Min , Ryu Jae-Chun , Lee Young-Seak
Published: March 2023

Recently, semiconductor wastewater treatment has received much attention due to the emergence of environmental issues. Acid-resistant coatings are essential for metal prefilters used in semiconductor wastewater treatment. Perfluoroalkoxy alkane is mainly used as an acid-resistant coating agent, since PFA has inherent superhydrophobicity, water permeability is lowered. To solve this problem, the surface of the PFA-coated metal mesh was treated via an oxyfluorination method in which an injected mixed gas of fluorine and oxygen reacted with the surface functional groups. Surface analysis, water contact angle measurement, and water permeability tests were performed on the surface-treated PFA-coated mesh. Consequently, the superhydrophobic surface was effectively converted to a hydrophobic surface as the PFA coating layer was surface-modified with C-O-OH functional groups via the oxyfluorination reaction. As a result of using simulation solutions that float silica particles of various sizes, the permeability and particle removal rate of the surface-modified PFA-coated stainless-steel mesh were improved compared to those before surface modification. Therefore, the oxyfluorination treatment used in this study was suitable for improving the filtration performance of SiO2 microparticles in the PFA-coated stainless-steel mesh.
Novel Microwave-Assisted Synthesis of COFs: 2020–2022
Authors: Rodríguez-Carríllo Cristina , Benítez Miriam , El Haskouri Jamal , Amorós Pedro , Ros-Lis Jose V.
Published: March 2023

Covalent organic frameworks (COFs) have emerged as a new type of crystalline porous polymers of great interest. However, their preparation requires long reaction times. Microwave-assisted synthesis (MAS) offers an interesting approach to increasing the reaction rate of chemical processes. Thus, microwaves can be a key tool for the fast and scalable synthesis of COFs. Since our previous review on the topic, the preparation of COFs with microwaves has been evolving. Herein, we present a compilation of COFs studies and experiments published in the last three years on the synthesis of COFs using microwave-assisted synthesis as a source of energy. The articles include imine, triazine, and other 2D COFs synthesized using MAS. The 3D COFs have also been compiled. The chemical structure of the monomers and the COFs and their main parameters of synthesis and application are summarized for each article.
Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides
Authors: Wang Zefen , Zhou Qian , Liu Siyuan , Liao Dankui , Liu Pengru , Lan Xiongdiao
Published: March 2023

Immobilized angiotensin-converting enzyme (ACE) is a promising material for the rapid screening of antihypertensive drugs, but the nonspecific adsorption is a serious problem in separation processes involving complex biological products. In this study, triblock copolymers with dopamine (DA) block as anchors and PEG block as the main body (DA-PEGx-DA) were attached to an immobilized ACE (ACE@mZIF-8/PDA, AmZP) surface via the “grafting to” strategy which endowed them with anti-nonspecific adsorption. The influence of DA-PEGx-DA chain length on nonspecific adsorption was confirmed. The excellent specificity and reusability of the obtained ACE@mZIF-8/PDA/DA-PEG5000-DA (AmZPP5000) was validated by screening two known ACE inhibitory peptides Val-Pro-Pro (VPP, competitive inhibitory peptides of ACE) and Gly-Met-Lys-Cys-Ala-Phe (GF-6, noncompetitive inhibitory peptides of ACE) from a mixture containing active and inactive compounds. These results demonstrate that anchored polymer loops are effective for high-recognition selectivity and AmZPP5000 is a promising compound for the efficient separation of ACE inhibitors in biological samples.
Antibacterial Efficacy of N-(4-methylpyridin-2-yl) Thiophene-2-Carboxamide Analogues against Extended-Spectrum-β-Lactamase Producing Clinical Strain of Escherichia coli ST 131
Authors: Ahmad Gulraiz , Khalid Aqsa , Qamar Muhammad Usman , Rasool Nasir , Saadullah Malik , Bilal Muhammad , Bajaber Majed A. , Obaidullah Ahmad J. , Alotaibi Hadil Faris , Alotaibi Jawaher M.
Published: March 2023

Development in the fields of natural-product-derived and synthetic small molecules is in stark contrast to the ongoing demand for novel antimicrobials to treat life-threatening infections caused by extended-spectrum β-lactamase producing Escherichia coli (ESBL E. coli). Therefore, there is an interest in the antibacterial activities of synthesized N-(4-methylpyridin-2-yl) thiophene-2-carboxamides (4a–h) against ESBL-producing E. coli ST131 strains. A blood sample was obtained from a suspected septicemia patient and processed in the Bactec Alert system. The isolate’s identification and antibacterial profile were determined using the VITEK 2® compact system. Multi-locus sequence typing of E. coli was conducted by identifying housekeeping genes, while ESBL phenotype detection was performed according to CLSI guidelines. Additionally, PCR was carried out to detect the blaCTX-M gene molecularly. Moreover, molecular docking studies of synthesized compounds (4a–h) demonstrated the binding pocket residues involved in the active site of the β-lactamase receptor of E. coli. The result confirmed the detection of E. coli ST131 from septicemia patients. The isolates were identified as ESBL producers carrying the blaCTX-M gene, which provided resistance against cephalosporins and beta-lactam inhibitors but sensitivity to carbapenems. Among the compounds tested, 4a and 4c exhibited high activity and demonstrated the best fit and interactions with the binding pocket of the β-lactamase enzyme. Interestingly, the maximum of the docking confirmations binds at a similar pocket region, further strengthening the importance of binding residues. Hence, the in vitro and molecular docking studies reflect the promising antibacterial effects of 4a and 4c compounds.
Comparative Analysis of Guatemalan and Qing Dynasty Jadeite Elemental Signs
Authors: Liu Xinxin , Zhou Qishen , Wang Yanlin , Shu Jun , Pan Shaokui , Zhan Fangmin
Published: March 2023

Different jadeites have different characteristics. In this paper, the La-ICP-MS test is used to compare and analyze the elemental characteristics of jadeite in Guatemala and the Qing dynasty. The test results show that the highest value of Guatemalan jadeite Ca can reach 2.5 apfu, while the highest value of Qing dynasty jadeite is 0.73 apfu. The highest value of Na is the same for both. The concentration distribution range and highest value of Guatemalan jadeite and Qing dynasty jadeite Mg/(Mg + Fe) are the same. Guatemalan jadeite and Qing dynasty jadeite have a very wide content of trace elements. Qing dynasty Ca/(Mg + Fe) distribution is wider. Concentrations of Guatemalan and Qing dynasty jadeite Sr/Ba, which is a marine sediment, are greater than 1. The Ba in the Qing dynasty jadeite sediments contains a large amount of clay, resulting in higher levels than the average amount in Guatemalan jadeite Ba. The standard distribution map is similar, showing a “horn” shape. The Sr distribution is uneven. Guatemalan jadeite is heavily enriched in rare earths. Eu shows positive and negative abnormalities. The total rare earth value is 8.15 ppm. Qing Dynasty jadeite shows light rare earth enrichment, and Eu is a positive anomaly. The total rare earth value is 7.07 ppm. The characteristics of the two elements are somewhat similar, but different, which does not rule out the possibility that Qing dynasty jadeite came from Guatemala.