bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative assessment of the effects of DREADDs and endogenously expressed GPCRs in hippocampal astrocytes on synaptic activity and memory
Authors: Lee Sophie H. , Mak Aline , Verheijen Mark H. G.
Published: March 2023

Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) have proven themselves as one of the key in vivo techniques of modern neuroscience, allowing for unprecedented access to cellular manipulations in living animals. With respect to astrocyte research, DREADDs have become a popular method to examine the functional aspects of astrocyte activity, particularly G-protein coupled receptor (GPCR)-mediated intracellular calcium (Ca2+) and cyclic adenosine monophosphate (cAMP) dynamics. With this method it has become possible to directly link the physiological aspects of astrocytic function to cognitive processes such as memory. As a result, a multitude of studies have explored the impact of DREADD activation in astrocytes on synaptic activity and memory. However, the emergence of varying results prompts us to reconsider the degree to which DREADDs expressed in astrocytes accurately mimic endogenous GPCR activity. Here we compare the major downstream signaling mechanisms, synaptic, and behavioral effects of stimulating Gq-, Gs-, and Gi-DREADDs in hippocampal astrocytes of adult mice to those of endogenously expressed GPCRs.
Chemical composition and anti-cholesterol activity of tea (Camellia sinensis) flowers from albino cultivars
Authors: Gao Ying , Han Zhen , Xu Yong-Quan , Yin Jun-Feng
Published: March 2023

Albino tea cultivars are mutant tea plants with altered metabolisms. Current studies focus on the leaves while little is known about the flowers. To evaluate tea flowers from different albino cultivars, the chemical composition and anti-cholesterol activity of tea flowers from three albino cultivars (i.e., Baiye No.1, Huangjinya, and Yujinxiang) were compared. According to the results, tea flowers from Yujinxiang had more amino acids but less polyphenols than tea flowers from the other two albino cultivars. A reduced content of procyanidins and a high chakasaponins/floratheasaponins ratio were characteristics of tea flowers from Yujinxiang. In vitro anti-cholesterol activity assays revealed that tea flowers from Yujinxiang exhibited stronger activity in decreasing the micellar cholesterol solubility, but not in cholesterol esterase inhibition and bile salt binding. It was noteworthy that there were no specific differences on the chemical composition and anti-cholesterol activity between tea flowers from albino cultivars and from Jiukeng (a non-albino cultivar). These results increase our knowledges on tea flowers from different albino cultivars and help food manufacturers in the cultivar selection of tea flowers for use.
Exposure to proton pump inhibitors is associated with the development of pediatric autoimmune diseases
Authors: Räisänen Laura , Viljakainen Heli , Kolho Kaija-Leena
Published: March 2023

Proton pump inhibitors (PPIs) have been associated with decreased gut microbiota diversity. Disrupted gut microbiota composition has been reported in several autoimmune diseases (ADs), such as type 1 diabetes mellitus (DM), autoimmune thyroiditis (AIT), juvenile idiopathic arthritis (JIA), and inflammatory bowel diseases (IBD). We investigated whether PPIs are associated with the development of ADs in children and concluded that PPI exposures could be related to the onset of ADs, especially IBD and potentially AIT as well.
Assessment of the dual role of Lyonia ovalifolia (Wall.) Drude in inhibiting AGEs and enhancing GLUT4 translocation through LC-ESI-QTOF-MS/MS determination and in silico studies
Authors: Sahu Neha , Singh Nitesh , Arya Kamal Ram , Reddy Sabbu Sathish , Rai Amit Kumar , Shukla Vijaya , Pandey Jyotsana , Narender Tadigoppula , Tamrakar Akhilesh Kumar , Kumar Brijesh , Rajak Bikash Kumar , Malik Sumira , Rustagi Sarvesh
Published: March 2023

Introduction: Diabetes mellitus (DM) is a metabolic disorder that results in glucose accumulation in the blood, accompanied by the production of advanced glycation end products (AGEs) through glycation of cellular proteins. These AGEs interfere with insulin signaling and prevent GLUT4 membrane translocation, thereby promoting the accumulation of more glucose in the blood and causing post-diabetic complications.

Methods: In this study, we examine the anti-diabetic potential of Lyonia ovalifolia (Wall.) Drude, a well-known ethnomedicinal plant of the Indian Himalayas. Considering its various medicinal properties, we analyzed its ethanolic extract and various solvent fractions for in vitro antiglycation activity and antidiabetic potential, i.e., stimulation of GLUT4 translocation.

Result and Discussions: The results showed that the extract and fractions exhibited increased antiglycation activity and an increased level of GLUT4 translocation. Analysis of a further 12 bioactive compounds of ethanolic extract, identified through LC-ESI-QTOF-MS/MS, revealed the presence of three new compounds: leucothol B, rhodoterpenoids A, and leucothol A. Moreover, we performed molecular docking of identified compounds against key proteins of diabetes mellitus: the sirtuin family of NAD (+)-dependent protein deacetylases 6 (SIRT6), aldose reductase (AR), and tyrosine kinase (TK). The results showed that flavonoid luteolin showed the best binding affinity ((−12.3 kcal/mol), followed by eriodictyol, astilbin, and syringaresinol. An ADMET study showed that luteolin, eriodictyol, astilbin, and syringaresinol may be promising drug candidates belonging to the flavonoid class of compounds, with no harmful effects and complying with all the drug-likeness guidelines. Furthermore, molecular dynamics (MD) simulations on a 50 ns timescale revealed that AR protein was most stable with luteolin throughout the simulation period. Therefore, this study reveals for the first time that L. ovalifolia plays an important role in insulin homeostasis, as shown in in vitro and in silico studies.
Resilient cultural practices for cognitive development during childhood within learning pathways with support from mediated mutual reciprocal theory
Authors: Tchombe Therese Mungah Shalo
Published: March 2023

Introduction

This paper examines resilient cultural practices, informed by cultural values for cognitive development in socialisation during childhood within the learning pathways model. It argues that the active role of children in their learning is not well captured or explained by theories or even empirical data. Learning pathways as a model in this paper is significant because it orients thinking on the fact that all activities children engage in always have social, psychological, and physical implications for cognitive enrichment. The activities are driven by resilient cultural practices informed by cultural norms, beliefs, and values such as responsible leadership qualities and cultivating socio-emotional and moral balance. With the above reasoning, this paper is underpinned by an empirical study with six specific objectives supported by Mediated Mutual Reciprocity theory.

Methods

The study used a mixed research design and conveniently selected a sample of 73 parents and teachers whose ages ranged from 25-50 years. A questionnaire and an interview guide were used for data collection. The three-sections questionnaire was constructed to find out information about resilient cultural practices and cognitive development. The items in sections two and three were rated on a 5 points Likert scale based on the occurrence of practices and behaviours. The interview guide was constructed to find out information on the three learning pathways; physical, social and psychological that are relevant to children in African cultures. Data were analysed using frequency and proportions and Multiple Regression Analysis to aggregate scores for given conceptual components. Analysis of qualitative data followed the systematic process of thematic and content analysis.

Results

Based on qualitative findings, it was realised that African children are exposed to physical, social, and psychological pathways to learning. Quantitative results showed that 85.5% of respondents are high in their resilient cultural practices whereas 14.5% of them are low in resilient cultural practices. In the same line, 74.4% of respondents’ cognitive development is high while a proportion of 25.6% is low. The inferential statistics showed that resilient cultural practices are significantly predictive of cognitive processes, reasoning, skills, and strategies at a 0.000 level of significance.

Discussion

Based on the use of the principles of the Mediated Mutual Reciprocal Theory, the study affirms the importance of children’s contributory role in their learning and cognitive development. The link between pathways, resilient cultural practices, and cognitive development highlights the significance of children’s involvement in their development through participation.
Overbenefitting, underbenefitting, and balanced: Different effort–reward profiles and their relationship with employee well-being, mental health, and job attitudes among young employees
Authors: Li Jie , Kaltiainen Janne , Hakanen Jari J.
Published: March 2023

We aimed to identify different, both balanced and imbalanced, effort–reward profiles and their relations to several indicators of employee well-being (work engagement, job satisfaction, job boredom, and burnout), mental health (positive functioning, life satisfaction, anxiety, and depression symptoms), and job attitudes (organizational identification and turnover intention). We examined data drawn randomly from Finnish population (n = 1,357) of young adults (23–34 years of age) collected in the summer of 2021 with quantitative methods. Latent profile analysis revealed three emerging groups in the data characterized by different combinations of efforts and rewards: underbenefitting (16%, high effort/low reward), overbenefitting (34%, low effort/high reward), and balanced employees (50%, same levels of efforts and rewards). Underbenefitting employees reported poorest employee well-being and mental health, and more negative job attitudes. In general, balanced employees fared slightly better than overbenefitting employees. Balanced employees experienced higher work engagement, life satisfaction, and less depression symptoms. The findings highlight the importance of balancing work efforts with sufficient rewards so that neither outweighs the other. This study suggests that the current effort–reward model would benefit from conceptualizing the previously ignored perspective of overbenefitting state and from considering professional development as one of the essential rewards at work.
When colleague got recognized: Third-party’s reaction to witnessing employee recognition
Authors: Yang Tianfei , Jiang Xia
Published: March 2023

Employee recognition, an incentive method widely used in management practice, plays an important role in the organization. Although extant studies have confirmed its effectiveness, little attention has been paid to its spillover effect. Based on the Social Cognitive Theory and Affective Events Theory, this study argues that employee recognition encounter can trigger cognitive and behavioral reactions. Through perceived organizational justice and workplace wellbeing, a chain-mediating effect connects witnessing employee recognition to work engagement. This research adopts the empirical research method by asking the participants to do the weekly survey (four times in 1 month), and 258 samples are collected. Using SPSS20.0 and its PROCESS macro module, hypotheses are tested. The results indicate (1) employees who witness leaders’ recognition of colleagues will (a) perceive higher organizational justice and (b) be more engaged in work. (2) Perceived organizational justice will mediate the positive relationship between employee recognition encounters with (a) workplace wellbeing and (b) work engagement. (3) Perceived organizational justice and workplace wellbeing will have a chain-mediating effect in the path of employee recognition encounter and work engagement. The results provide both practical and theoretical contribution to employee recognition.
Turing instability mechanism of short-memory formation in multilayer FitzHugh-Nagumo network
Authors: Wang Junjie , Shen Jianwei
Published: March 2023

Introduction

The study of brain function has been favored by scientists, but the mechanism of short-term memory formation has yet to be precise.

Research problem

Since the formation of short-term memories depends on neuronal activity, we try to explain the mechanism from the neuron level in this paper.

Research contents and methods

Due to the modular structures of the brain, we analyze the pattern properties of the FitzHugh-Nagumo model (FHN) on a multilayer network (coupled by a random network). The conditions of short-term memory formation in the multilayer FHN model are obtained. Then the time delay is introduced to more closely match patterns of brain activity. The properties of periodic solutions are obtained by the central manifold theorem.

Conclusion

When the diffusion coeffcient, noise intensity np, and network connection probability p reach a specific range, the brain forms a relatively vague memory. It is found that network and time delay can induce complex cluster dynamics. And the synchrony increases with the increase of p. That is, short-term memory becomes clearer.
Prioritisation processes for programme implementation and evaluation in public health: A scoping review
Authors: Atwal Shaileen , Schmider Jessica , Buchberger Barbara , Boshnakova Anelia , Cook Rob , White Alicia , El Bcheraoui Charbel
Published: March 2023

Background

Programme evaluation is an essential and systematic activity for improving public health programmes through useful, feasible, ethical, and accurate methods. Finite budgets require prioritisation of which programmes can be funded, first, for implementation, and second, evaluation. While criteria for programme funding have been discussed in the literature, a similar discussion around criteria for which programmes are to be evaluated is limited. We reviewed the criteria and frameworks used for prioritisation in public health more broadly, and those used in the prioritisation of programmes for evaluation. We also report on stakeholder involvement in prioritisation processes, and evidence on the use and utility of the frameworks or sets of criteria identified. Our review aims to inform discussion around which criteria and domains are best suited for the prioritisation of public health programmes for evaluation.

Methods

We reviewed the peer-reviewed literature through OVID MEDLINE (PubMed) on 11 March 2022. We also searched the grey literature through Google and across key websites including World Health Organization (WHO), US Centers for Disease Control and Prevention (CDC), European Centre for Disease Prevention and Control (ECDC), and the International Association of National Public Health Institutes (IANPHI) (14 March 2022). Articles were limited to those published between 2002 and March 2022, in English, French or German.

Results

We extracted over 300 unique criteria from 40 studies included in the analysis. These criteria were categorised into 16 high-level conceptual domains to allow synthesis of the findings. The domains most frequently considered in the studies were “burden of disease” (33 studies), “social considerations” (30 studies) and “health impacts of the intervention” (28 studies). We only identified one paper which proposed criteria for use in the prioritisation of public health programmes for evaluation. Few prioritisation frameworks had evidence of use outside of the setting in which they were developed, and there was limited assessment of their utility. The existing evidence suggested that prioritisation frameworks can be used successfully in budget allocation, and have been reported to make prioritisation more robust, systematic, transparent, and collaborative.

Conclusion

Our findings reflect the complexity of prioritisation in public health. Development of a framework for the prioritisation of programmes to be evaluated would fill an evidence gap, as would formal assessment of its utility. The process itself should be formal and transparent, with the aim of engaging a diverse group of stakeholders including patient/public representatives.
Epidemiological pattern of COVID-19 and its association with periodontal health in an urban Indian cohort
Authors: Gupta Ishita , Patel Shivani A. , Kondal Dimple , Goodman Michael , Mohan Sailesh , Ali Mohammed K. , Tandon Nikhil , Narayan K. M. Venkat , Prabhakaran Dorairaj , Shridhar Krithiga
Published: March 2023

Background

Studies have highlighted a possible influence of gingival and periodontal disease (PD) on COVID-19 risk and severity. However, the evidence is based on hospital-based studies and community-level data are sparse.

Objectives

We described the epidemiological pattern of SARS-CoV-2 infection in Delhi and evaluated the associations of gingival and PD with incident COVID-19 disease in a regionally representative urban Indian population.

Methods

In a prospective study nested within the Centre for Cardiometabolic Risk Reduction in South-Asia (CARRS) study, participants with clinical gingival and periodontal status available at baseline (2014–16) (n = 1,727) were approached between October 2021 to March 2022. Information on COVID-19 incidence, testing, management, severity was collected as per the WHO case criteria along with COVID-19 vaccination status. Absolute incidence of COVID-19 disease was computed by age, sex, and oral health. Differences in rates were tested using log-rank test. Poisson regression models were used to evaluate independent associations between gingival and PD and incidence of COVID-19, adjusted for socio-demographic and behavioral factors, presence of comorbidity, and medication use.

Results

Among 1,727 participants, the mean age was 44.0 years, 45.7% were men, 84.5% participants had baseline gingival or PD and 89.4% participants had received at least one dose of COVID-19 vaccine. Overall, 35% (n = 606) participants were tested for COVID-19 and 24% (n = 146/606) tested positive. As per the WHO criteria total number of cases was 210, constituting 12% of the total population. The age and sex-specific rates of COVID-19 were higher among men and older participants, but women aged >60 years had higher rates than men of same age. The incidence rate did not differ significantly between those having gingival or PD and healthy periodontium (19.1 vs. 16.5/1,000 person-years) and there was no difference in risk of COVID-19 by baseline oral disease status.

Conclusion

Gingival and PD were not associated with increased risk of COVID-19.
Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol
Authors: Wang Jiaying , Chen Zhiqiang , Deng Xiaogui , Yuan Qianqian , Ma Hongwu
Published: March 2023

The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2. We constructed a methanol assimilation pathway to produce poly-3-hydroxybutyrate (P3HB) using the MCC pathway, which included the ribulose monophosphate (RuMP) pathway for methanol assimilation and non-oxidative glycolysis (NOG) for acetyl-CoA (precursor for PHB synthesis) production. The theoretical product carbon yield of the new pathway is 100%, hence no carbon loss. We constructed this pathway in E. coli JM109 by introducing methanol dehydrogenase (Mdh), a fused Hps–phi (hexulose-6-phosphate synthase and 3-phospho-6-hexuloisomerase), phosphoketolase, and the genes for PHB synthesis. We also knocked out the frmA gene (encoding formaldehyde dehydrogenase) to prevent the dehydrogenation of formaldehyde to formate. Mdh is the primary rate-limiting enzyme in methanol uptake; thus, we compared the activities of three Mdhs in vitro and in vivo and then selected the one from Bacillus methanolicus MGA3 for further study. Experimental results indicate that, in agreement with the computational analysis results, the introduction of the NOG pathway is essential for improving PHB production (65% increase in PHB concentration, up to 6.19% of dry cell weight). We demonstrated that PHB can be produced from methanol via metabolic engineering, which provides the foundation for the future large-scale use of one-carbon compounds for biopolymer production.
Socket Preservation after Tooth Extraction: Particulate Autologous Bone vs. Deproteinized Bovine Bone
Authors: Chisci Glauco , Hatia Arjeta , Chisci Elettra , Chisci Dafne , Gennaro Paolo , Gabriele Guido
Published: March 2023

Background: The technique of socket preservation after tooth extraction allows for less volumetric decrease after tooth extraction. The aim of this retrospective study was to evaluate differences between alveolar socket preservation performed with deproteinized bovine bone graft and autologous particulate bone graft taken from the mandibular ramus. Materials and Methods: This retrospective study enrolled a total of 21 consecutive patients. A total of 11 patients underwent socket preservation with deproteinized bovine bone graft and collagen matrix (group A), and 10 patients underwent socket preservation performed with particulate autologous bone taken from the mandibular ramus and collagen matrix (group B). All patients received cone beam computed tomography (CBCT) before socket preservation and after four months. Alveolar bone width (ABW) values and alveolar bone height (ABH) values were measured at the first and second CBCT, and the reduction of the values in the two groups was compared. Statistical analysis was performed using Student’s t-test for independent variables, and p values < 0.05 were considered statistically significant. Results: There were no statistically significant differences between ABW reduction of group A and ABW reduction of group B (t-test value p = 0.28). There were no statistically significant differences between ABH reduction of group A and ABH reduction of group B (t-test value p = 0.10). Conclusions: In this retrospective study, no statistical differences were found between the group that received autologous particulate bone compared to the group that received deproteinized bovine bone in socket preservation.
The Effects of PP2A Disruption on ER-Mitochondria Contact and Mitochondrial Functions in Neuronal-like Cells
Authors: Chaiwijit Phaewa , Uppakara Kwanchanok , Asavapanumas Nithi , Saengsawang Witchuda
Published: March 2023

Mitochondria-associated membranes (MAMs) regulate several cellular processes, including calcium homeostasis and mitochondrial function, and dynamics. While MAMs are upregulated in Alzheimer’s disease (AD), the mechanisms underlying this increase remain unknown. A possible mechanism may include dysregulation of protein phosphatase 2A (PP2A), which is reduced in the AD brain. Furthermore, PP2A has been previously reported to modulate MAM formation in hepatocytes. However, it is unknown whether PP2A and MAMs are linked in neuronal cells. Here, to test the correlation between PP2A and MAMs, we inhibited the activity of PP2A to mimic its low levels in AD brains and observed MAM formation, function, and dynamics. MAMs were significantly increased after PP2A inhibition, which correlated with elevated mitochondrial Ca2+ influx and disrupted mitochondrial membrane potential and mitochondrial fission. This study highlights the essential role PP2A plays in regulating MAM formation and mitochondrial function and dynamics for the first time in neuronal-like cells.
Compound 13 Promotes Epidermal Healing in Mouse Fetuses via Activation of AMPK
Authors: Takaya Kento , Okabe Keisuke , Sakai Shigeki , Aramaki-Hattori Noriko , Asou Toru , Kishi Kazuo
Published: March 2023

Unlike adults, early developing fetuses can completely regenerate tissue, and replicating this could lead to the development of treatments to reduce scarring. Mice epidermal structures, including wound healing patterns, are regenerated until embryonic day (E) 13, leaving visible scars thereafter. These patterns require actin cable formation at the epithelial wound margin through AMP-activated protein kinase (AMPK) activation. We aimed to investigate whether the administration of compound 13 (C13), a recently discovered AMPK activator, to the wound could reproduce this actin remodeling and skin regeneration pattern through its AMPK activating effect. The C13 administration resulted in partial formations of actin cables, which would normally result in scarring, and scar reduction during the healing of full-layer skin defects that occurred in E14 and E15 fetuses. Furthermore, C13 was found to cause AMPK activation in these embryonic mouse epidermal cells. Along with AMPK activation, Rac1 signaling, which is involved in leaflet pseudopodia formation and cell migration, was suppressed in C13-treated wounds, indicating that C13 inhibits epidermal cell migration. This suggests that actin may be mobilized by C13 for cable formation. Administration of C13 to wounds may achieve wound healing similar to regenerative wound healing patterns and may be a potential candidate for new treatments to heal scars.
LncRNA SNHG4 Modulates EMT Signal and Antitumor Effects in Endometrial Cancer through Transcription Factor SP-1
Authors: Kim Lee Kyung , Park Sun-Ae , Nam Eun Ji , Kim Young Tae , Heo Tae-Hwe , Kim Hee Jung
Published: March 2023

Long non-coding RNAs (lncRNAs) are implicated in the initiation and progression of a variety of tumors, including endometrial cancer. However, the mechanisms of lncRNA in endometrial cancer formation and progression remain largely unknown. In this study, we confirmed that the lncRNA SNHG4 is upregulated in endometrial cancer and correlates with lower survival rates in endometrial cancer patients. Knock-down of SNHG4 significantly reduced cell proliferation, colonization, migration, and invasion in vitro, as well as modulating the cell cycle and reduced tumor growth of endometrial cancer in vivo. In addition, the effect of SNHG4 by the transcription factor SP-1 was confirmed in vitro. We found in this study that SNHG4/SP-1 plays an important role in endometrial cancer progression and may be used as a potential therapeutic and prognostic biomarker for endometrial cancer.
Systemic Cytokine Expression in Diabetes Is Associated with Prolonged Gastrointestinal Transit Times and Cardinal Gastroparesis Symptoms
Authors: Okdahl Tina , Wegeberg Anne-Marie , Jensen Anne Birthe Helweg , Jensen Sarah Thorius , Andersen Helene Riis Pontoppidan , Størling Joachim , Brock Birgitte , Brock Christina
Published: March 2023

Gastroenteropathy is a common complication in diabetes associated with damages to the enteric nervous system. Systemic low-grade inflammation facilitates neurotoxicity, and associations with peripheral and autonomic neuropathy have been reported. However, less is known of associations with gastroenteropathy. To explore the area cross-sectionally, we included individuals with diabetes (type 1: 56, type 2: 100) and 21 healthy controls. Serum levels of interleukin (IL)-6, IL-8, IL-10, tumour necrosis factor (TNF)-α, and interferon (IFN)-γ were measured by multiplex technology. Segmental gastrointestinal transit times were assessed by wireless motility capsule investigations. Symptoms of gastroparesis were rated on Gastroparesis Cardinal Symptom Index questionnaires. Compared to healthy, levels of TNF-α were decreased in type 1 diabetes and increased in type 2 diabetes, while colonic transit time was increased (all p < 0.05). In diabetes, associations between IL-8 and prolonged gastric emptying (odds ratio (OR) 1.07, p = 0.027) and between IL-10 and prolonged colonic transit (OR 29.99, p = 0.013) were seen. Inverse correlations between IL-6 and nausea/vomiting (rho = −0.19, p = 0.026) and bloating (rho = −0.29; p < 0.001) were found. These findings indicate a plausible interaction between inflammation and the enteric nervous system in diabetes, which raises the question of whether anti-inflammatory strategies could be applied in management of diabetic gastroenteropathy.
Sodium Lauryl Sulfate-Conjugated Cationic Gemini-Surfactant-Capped Gold Nanoparticles as Model System for Biomolecule Recognition
Authors: Grueso Elia , Giráldez-Pérez Rosa M. , Prado-Gotor Rafael , Kuliszewska Edyta
Published: March 2023

Surfactant-based nanostructures are promising materials for designing novel colorimetric biosensors based on aggregation/disaggregation phenomena. In this work, a colorimetric sensor based on the plasmonic shift of surfactant-capped gold nanoparticles via the disaggregation mechanism was developed. To perform this, the optimum SDS concentration was firstly determined in order to form Au@16-s-16/SDS complex aggregates with a well-defined SPR band in the blue region. Once the optimal SDS concentration for Au@16-s-16 aggregation was established, the sensing method depended on the nature of the electrostatic charge of the biopolymer studied where both the strength of the biopolymer/SDS and biopolymer/Au@16-s-16 interactions and the cationic gold nanoparticles play a key role in the disaggregation processes. As a result, an instantaneous color change from blue to red was gradually observed with increasing biopolymer concentrations. The response of the sensor was immediate, avoiding problems derived from time lapse, and highly dependent on the order of addition of the reagents, with a detection limit in the nanomolar and picomolar range for DNA and Lysozyme sensing, respectively. This behavior can be correlated with the formation of different highly stabilized Au@16-s-16/biopolymer/SDS complexes, in which the particular biopolymer conformation enhances the distance between Au@16-s-16 nanoparticles among the complexes.
Association between Weight Status and Mental Health among Korean Adolescents: A Nationwide Cross-Sectional Study
Authors: Choi Youngha , Hong Jeana
Published: March 2023

This study explored the relationship between adolescents’ weight status and mental health problems. It specifically investigated the weight perceptions of obese adolescents and the effect on their mental health. This cross-sectional study was based on the data of adolescents aged 12–18 years from the Korean National Health and Nutritional Examination Survey (2010–2019). Data regarding anthropometric measurements, health conditions, and socioeconomic status were extracted, and the associations between weight status (actual, perceived, or misperceived) and mental health conditions (depressed mood, perceived stress, and suicidal ideation) were analyzed using complex sample multiple logistic regression after adjusting for possible confounders. A total of 5683 adolescents (53.1% boys and 46.9% girls) were included in this study, with a mean age of 15.1 years. Among the participants, actual, perceived, and misperceived status of being overweight were observed in 20.8%, 32.7%, and 18.4%, respectively. Additionally, depressed mood, perceived stress, and suicidal ideation were observed in 9.1%, 25.7%, and 7.4% of Korean adolescents, respectively, with higher prevalences in girls for all three conditions. Actual weight status was not significantly associated with mental health conditions in either sex. Furthermore, girls who perceived themselves to be overweight, regardless of their actual body weight, or who had overestimated their actual weight were more likely to have experienced depressed mood and stress, while boys who perceived themselves to be underweight were more likely to have experienced suicidal ideation than participants with an average weight perception or an accurate recognition of their weight status. Conversely, in overweight/obese participants, perceived weight status was not associated with mental health conditions. In conclusion, perceived weight status and its discrepancy with actual body weight were more strongly associated with an increased risk of mental health problems than actual weight status itself among Korean adolescents. Therefore, adolescents’ perceptions of their body image and weight-related attitude should be assessed to promote their mental health.
Satellite-Derived Annual Glacier Surface Flow Velocity Products for the European Alps, 2015–2021
Authors: Rabatel Antoine , Ducasse Etienne , Millan Romain , Mouginot Jérémie
Published: March 2023

Documenting glacier surface flow velocity from a longer-term perspective is highly relevant to evaluate the past and current state of glaciers worldwide. For this purpose, satellite data are widely used to obtain region-wide coverage of glacier velocity data. Well-established image correlation methods allow for the automated measurement of glacier surface displacements from satellite data (optical and radar) acquired at different dates. Although computationally expensive, image correlation is nowadays relatively simple to implement and allows two-dimensional displacement measurements. Here, we present a data set of annual glacier surface flow velocity maps at the European Alps scale, covering the period 2015–2021 at a 50 m × 50 m resolution. This data set has been quantified by applying the normalized cross-correlation approach on Sentinel-2 optical data. Parameters of the cross-correlation method (e.g., window size, sampling resolution) have been optimized, and the results have been validated by comparing them with in situ data on monitored glaciers showing an RMSE of 10 m/yr. These data can be used to evaluate glacier dynamics and its spatial and temporal evolution (e.g., quantify mass fluxes or calving) or can be used as an input for model calibration/validation or for the early detection of regional hazards associated with glacier destabilization.
Sequence Segmentation Attention Network for Skeleton-Based Action Recognition
Authors: Zhang Yujie , Cai Haibin
Published: March 2023

With skeleton-based action recognition, it is crucial to recognize the dependencies among joints. However, the current methods are not able to capture the relativity of the various joints among the frames, which is extremely helpful because various parts of the body are moving at the same time. In order to solve this problem, a new sequence segmentation attention network (SSAN) is presented. The successive frames are encoded in each of the segments that make up the skeleton sequence. Then, we provide a self-attention block that may record the associated information among various joints in successive frames. In order to better recognize comparable behavior, a model of external segment action attention is employed to acquire the deep interrelation information among parts. Compared with the most advanced approaches, we have shown that the proposed method performs better on NTU RGB+D and NTU RGB+D 120.
Improved Extraction Yield, Water Solubility, and Antioxidant Activity of Lentinan from Lentinula edodes via Bacillus subtilis natto Fermentation
Authors: Xu Mengyue , Qu Yaning , Li Hui , Tang Shuangqing , Chen Chanyou , Wang Yazhen , Wang Hongbo
Published: March 2023

Lentinan has important applications in the food and medicine fields. Fermenting Lentinula edodes with Bacillus subtilis natto increased the lentinan extraction yield by 87.13% and greatly altered the molecular structure and antioxidant activity of lentinan. The uronic acid content in the lentinan molecular structure increased from 2.08% to 4.33%. The fermentation process did not affect the monosaccharide composition of lentinan, comprised of more than 90% glucose residues. Fermentation significantly reduced the molecular weight of lentinan and altered its apparent structure. The water solubility of fermented lentinan was increased by 165.07%, and the antioxidant activity was significantly improved. Fermentation using soybean as a substrate may be beneficial for enhancing the activity of Bacillus subtilis natto and producing lentinan with different molecular weights.
Application of Conjoint Analysis and 5Ps Marketing Mix in Identifying Customer Preference of Alcohol Consumption among Youths in the Philippines
Authors: Yandug Jenalyn Shigella G. , Ponce France D. , Ong Ardvin Kester S.
Published: March 2023

Alcoholic beverages have long been one of the most popular commodities. They have been mass-produced worldwide because of their popularity and demand. On a survey by Statista in 2021, alcohol consumption is projected to increase in the Philippines. Thus, the popularity of numerous alcoholic beverages piqued the curiosity of consumers and researchers alike. This study used conjoint analysis that aims to have a better understanding of consumer preferences for alcohol consumption among youths. Additionally, the study considered the following attributes: (1) type of alcoholic beverage, (2) oral sensation, (3) flavor, (4) origin, (5) color, and (6) price. Results revealed that the product’s price is the most crucial attribute influencing consumer preferences by 26.311%, followed by the type of alcoholic beverage with an importance score of 26.237%. The least considered attribute is the product’s color, having an importance score of 7.790%. These were supported by two statistical tests, the Pearson’s Correlation Test and Kendall’s Tau Test, which both obtained a value higher than 0.8. Managerial implications were presented in the study to help provide strategies and development for alcoholic beverage distribution in the Philippines based on the findings of this study, which relate to young consumers.
Design of Biodegradable Films Using Pecan Nut Cake Extracts for Food Packing
Authors: Alves Jamila dos Santos , Canabarro Nicholas Islongo , Boeira Caroline Pagnossim , Melo Pamela Thais Sousa , Aouada Marcia Regina de Moura , da Rosa Claudia Severo
Published: March 2023

The excessive consumption of plastic packaging and its consequent disposal and accumulation in the environment have aroused the interest of researchers in developing packaging that can cause less harm to nature. In this sense, this article presents research on the addition of antioxidant extracts from pecan nut cake in biodegradable packaging made with a polymeric mixture of gelatin and corn starch. The films produced were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, thickness, mechanical properties, water vapor permeability (WVP), solubility, water contact angle, optical properties, in vitro bioactive activity, and biodegradability. A higher concentration of total phenolic compounds (101.61 mg GAE/g) was found for the condition where alcohol content and extraction time were 65% and 20 min, respectively. Pecan nut cake (PNC( extracts did not influence the film’s tensile strength, and elongation at break was tightly increased by adding 10–20% extracts. The film’s characterization pointed to more than 67% solubility, and adding PNC extract implied more hydrophilic surfaces (contact angles lower than 65°). Furthermore, the film opacity showed a linear relation with PNC extract concentration, and a higher luminosity (L*) was observed for the film without extract. Furthermore, the antioxidant activity of the films was enhanced with the addition of PNC extracts, and complete biodegradation was observed until the ninth day. Therefore, biodegradable films prepared from a mixture of gelatin starch and enriched with PNC extracts showed excellent mechanical properties and potential as carriers of antioxidant compounds, allowing us to propose their use as active packing.
Techno-Functional and Rheological Properties of Alternative Plant-Based Flours
Authors: Badia-Olmos Celia , Laguna Laura , Haros Claudia Mónika , Tárrega Amparo
Published: March 2023

The use of alternative vegetal sources is a proposed strategy to improve the diversity and quality of plant-based products on the market, currently led by soy and pea. This study compares the techno-functional properties of seven vegetable flours (chickpea, lentil, red lentil, white bean, quinoa, amaranth, and oat) and the rheological properties of their flour pastes and gels. All techno-functional properties significantly (α = 0.05) varied depending on the type of flour. Among the flours studied, the highest swelling capacity was for white bean and the lowest for chickpea and red lentil. Water holding capacity was high for white bean and oat flours and low for red lentil. Oat and quinoa flours had the highest oil-holding capacity. Emulsifying and foaming capacities were high for all pulse flours but poor for amaranth and oat flours. However, amaranth and oat provided a much higher viscosity during heating than the rest of the flours. The viscoelastic properties of the flour pastes indicated that they all had a gel structure with storage modulus (G′) values over loss modulus (G″) values. From the viscoelastic properties, amaranth and quinoa showed a weak gel structure with low G′ and G″ values, and the chickpea, lentil, and red lentil formed pastes with a high elastic contribution (high G′ values). In agreement, these three pulse flours were the only ones able to form hard, self-standing gels. These results show the potential of vegetal flours from alternative sources in the development of new plant-based products.
Effects of pH and NaCl on the Spatial Structure and Conformation of Myofibrillar Proteins and the Emulsion Gel System—Insights from Computational Molecular Dynamics on Myosin of Golden Pompano
Authors: Xue Changfeng , Pei Zhisheng , Wen Pan , Chin Yaoxian , Hu Yaqin
Published: March 2023

In this study, the effects of pH and NaCl concentrations on the structure of golden pompano myosin and emulsion gel were analyzed using SEM in combination with molecular dynamics simulations (MDS). The microscopic morphology and spatial structure of myosin were investigated at different pH (3.0, 7.0, and 11.0) and NaCl concentrations (0.0, 0.2, 0.6, and 1.0 M), and their effects on the stability of emulsion gels were discussed. Our results show that pH had a greater effect on the microscopic morphology of myosin than NaCl. The MDS results show that under the condition of pH 7.0 and 0.6 M NaCl, the myosin expanded and experienced significant fluctuations in its amino acid residues. However, NaCl showed a greater effect on the number of hydrogen bonds than pH. Although changes in pH and NaCl concentrations only slightly altered the secondary structures in myosin, they, nevertheless, significantly influenced the protein spatial conformation. The stability of the emulsion gel was affected by pH changes but not NaCl concentrations, which only affect the rheology. The best elastic modulus G″ of the emulsion gel was obtained at pH 7.0 and 0.6 M NaCl. Based on the results, we conclude that pH changes have a greater influence than NaCl concentrations on the spatial structure and conformation of myosin, contributing to the instability of its emulsion gel state. The data from this study would serve as a valuable reference for emulsion gel rheology modification in future research.
Beyond Sol-Gel: Molecular Gels with Different Transitions
Authors: Yilmazer Senem , Schwaller Duncan , Mésini Philippe J.
Published: March 2023

The existence of sol–gel transitions is one of the most manifest properties of molecular gels. These transitions reflect their nature since they correspond to the association or dissociation of low weight molecules through non-covalent interactions to form the network constitutive of the gel. Most described molecular gels undergo only one gel-to-sol transition upon heating, and the reverse sol-to-gel transition upon cooling. It has been long observed that different conditions of formation could lead to gels with different morphologies, and that gels can undergo a transition from gel to crystals. However, more recent publications report molecular gels which exhibit additional transitions, for instance gel-to-gel transitions. This review surveys the molecular gels for which, in addition to sol–gel transitions, transitions of different nature have been reported: gel-to-gel transitions, gel-to-crystal transition, liquid–liquid phase separations, eutectic transformations, and synereses.
Cognitive Decline and Its Associated Factors in Patients with Major Depressive Disorder
Authors: Ab Latiff Husni Zaim , Ariaratnam Suthahar , Shuib Norley , Isa Mohamad Rodi
Published: March 2023

Background: Major Depressive Disorder (MDD) is a significant and common mental health problem occurring worldwide. Cognitive decline is frequently observed during acute and residual phases of MDD, contributing significantly to functional impairment. The aim of this study was to determine the clinical profile and correlates of cognitive decline amongst adult outpatients with MDD. Methods: The survey was cross-sectional in design. A systematic random sampling method was used to recruit patients. Confirmation of MDD was achieved by using the Mini International Neuropsychiatric Interview (M.I.N.I 7.0). Cognitive decline was measured using the Montreal Cognitive Assessment (MoCA). Descriptive analysis was performed, followed by univariate and multiple logistic regression analyses. Results: Out of 245 patients, 32.7% (n = 80, 95% CI: 26.7, 38.6) had cognitive decline. Multiple logistic regression showed the existence of cognitive decline amongst MDD patients, which was significantly associated with those having secondary and lower levels of education (OR: 6.09; 95% CI: 2.82, 13.16; p < 0.001), five or more depressive episodes (OR: 8.93; 95% CI: 3.24, 24.67; p < 0.001), treatment non-compliance (OR: 3.48; 95% CI: 1.40, 6.59; p = 0.003), and medical comorbidity (OR: 2.74; 95% CI: 1.46, 5.18; p = 0.002). Conclusions: Cognitive decline is a prevalent condition among outpatients with MDD. Clinicians need to be cognizant about measures of cognition and related risk factors. Timely control of both depression and medical comorbidities would be a reasonable approach to improve functional outcomes in MDD patients.
Genome-Wide Identification and Expression Analysis of the fw2.2-like Gene Family in Pear
Authors: Pu Xiaoqiu , Tian Jia , Li Jiang , Wen Yue
Published: March 2023

Fruit size is a major factor determining yield, quality, and consumer acceptability. fw2.2 (fruit weight-2.2) is a primary quantitative trait locus that was the first to be cloned, accounting for 30% of the variation in tomato fruit size. The various homologs of fw2.2 (fw2.2-like) have been identified in many plants and belong to a large family. To date, there has been no report that has carried out a comprehensive identification of fw2.2-like members in pear. In this study, a total of 14 fw2.2-like genes were identified in the pear (Pyrus bretschneideri Rehd) genome and designated as PbFWL1-14. All of the PbFWL genes were unevenly distributed on nine chromosomes, and each chromosome contained between one and four genes. All PbFWL proteins contained more than two conserved motifs, and PbFWL genes contained more than one intron, and the genes of the same subfamily seemed to have a similar intron gene structure. According to the neighbor-joining phylogenetic tree, a total of 78 fw2.2/CNR (cell number regulator) from five plant species, including pear, maize, tomato, peach, rice, and physalis, could be divided into seven subgroups, and PbFWL proteins were mainly distributed in subgroups 1, 3, 4, 5, and 6. The Ka/Ks analysis also revealed that the fw2.2-like gene family of pear may have been subjected to strong purifying selection pressure during its evolution. A cis-element analysis found that many cis-elements responsive to hormones and stress were discovered in promotion regions for all PbFWLs. When combining real-time quantitative PCR analysis detection results, PbFWL1/2/5 were found to be the most likely candidate genes for regulating pear fruit size.