bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Significance of Immune Checkpoints and EBV Reactivation in Antibody Deficiencies with Near-Normal Immunoglobulin Levels or Hyperimmunoglobulinemia
Authors: Mertowska Paulina , Mertowski Sebastian , Smolak Konrad , Pasiarski Marcin , Smok-Kalwat Jolanta , Góźdź Stanisław , Grywalska Ewelina
Published: October 2023

This study delves into the intricate landscape of primary immunodeficiencies, with a particular focus on antibody deficiencies characterized by near-normal immunoglobulin levels or hyperimmunoglobulinemia. Contrary to the conventional focus on genetic dysregulation, these studies investigate the key roles of immune checkpoints, such as PD-1/PD-L1, CTLA-4/CD86, and CD200R/CD200, on selected subpopulations of T and B lymphocytes and their serum concentrations of soluble forms in patients recruited for the studies in healthy volunteers. In addition, the studies also show the role of Epstein–Barr virus (EBV) reactivation and interactions with tested pathways of immune checkpoints involved in the immunopathogenesis of this disease. By examining the context of antibody deficiencies, this study sheds light on the nuanced interplay of factors beyond genetics, particularly the immune dysregulations that occur in the course of this type of disease and the potential role of EBV reactivation, which affects the clinical presentation of patients and may contribute to the development of cancer in the future, especially related to hematological malignancies.
Immunotherapy with Monoclonal Antibodies for Acute Myeloid Leukemia: A Work in Progress
Authors: Molica Matteo , Perrone Salvatore , Andriola Costanza , Rossi Marco
Published: October 2023

In the last few years, molecularly targeted agents and immune-based treatments (ITs) have significantly changed the landscape of anti-cancer therapy. Indeed, ITs have been proven to be very effective when used against metastatic solid tumors, for which outcomes are extremely poor when using standard approaches. Such a scenario has only been partially reproduced in hematologic malignancies. In the context of acute myeloid leukemia (AML), as innovative drugs are eagerly awaited in the relapsed/refractory setting, different ITs have been explored, but the results are still unsatisfactory. In this work, we will discuss the most important clinical studies to date that adopt ITs in AML, providing the basis to understand how this approach, although still in its infancy, may represent a promising therapeutic tool for the future treatment of AML patients.
The Prognosis Predictive Score around Neo Adjuvant Chemotherapy (PPSN) Improves Diagnostic Efficacy in Predicting the Prognosis of Epithelial Ovarian Cancer Patients
Authors: Kawahara Naoki , Yamanaka Shoichiro , Sugimoto Sumire , Kamibayashi Junya , Nishikawa Kyohei , Kawaguchi Ryuji , Kimura Fuminori
Published: October 2023

Background: Recent studies have shown that pretreatment inflammatory responses can predict prognosis. However, no reports have analyzed the combined effect of the inflammatory response with pre-treatment and post-neo adjuvant chemotherapy (NACT). This retrospective study aims to identify factors predicting prognosis and create a novel predictive scoring system. Methods: The study was conducted at our institution between June 2006 and March 2020. Demographic and clinicopathological data were collected from patients with advanced epithelial ovarian cancer who underwent neoadjuvant chemotherapy after sample collection by laparoscopic or laparotomy surgery, followed by interval debulking surgery. We created a scoring system, called the Predictive Prognosis Score around NACT (PPSN), using factors extracted from a receiver operating characteristic curve analysis. Univariate and multivariate analyses were conducted to assess the efficacy of PPSN in predicting progression-free survival and overall survival. Kaplan-Meier and log-rank tests were used to compare the PFS or OS rate. Results: Our study included 72 patients, with a cut-off value of four for the scoring system. Our analysis showed that high PPSN (≥4) significantly predicts poor prognosis. Moreover, CD3+ and CD8+ tumor-infiltrating lymphocytes with low PPSN (<4) showed higher aggregation than those with high PPSN (≥4) cases. Conclusion: Our study shows that PPSN could be a useful prognostic tool for advanced EOC patients who undergo NACT followed by IDS.
Measurable Residual Disease (MRD) by Flow Cytometry in Adult B-Acute Lymphoblastic Leukaemia (B-ALL) and Acute Myeloid Leukaemia (AML): Correlation with Molecular MRD Testing and Clinical Outcome at One Year
Authors: van der Linde Riana , Gatt Prudence N. , Smith Sandy , Fernandez Marian A. , Vaughan Lachlin , Blyth Emily , Curnow Jennifer , Brown David A. , Tegg Elizabeth , Sasson Sarah C.
Published: October 2023

Measurable residual disease (MRD) detected by flow cytometry (FC) is well established in paediatric B- lymphoblastic leukaemia (B-ALL) and adult chronic lymphocytic leukaemia (CLL), but its utility in adult B-ALL and adult acute myeloid leukaemia (AML) is less clear. In this prospective MRD study, one of the largest in Australia to date, we examined consecutive bone marrow aspirates from adult participants with B-ALL (n = 47) and AML (n = 87) sent for FC-MRD testing at a quaternary referral hospital in Sydney. FC-MRD results were correlated to corresponding Mol-MRD testing where available and clinical outcomes at three-month intervals over 1 year. B-ALL showed a moderate positive correlation (rs = 0.401, p < 0.001), while there was no correlation between FC-MRD and Mol-MRD for AML (rs = 0.13, p = 0.237). Five FC-MRD patterns were identified which had significant associations with relapse (X2(4) = 31.17(4), p > 0.001) and survival (X2(4) = 13.67, p = 0.008) in AML, but not in B-ALL. The three-month MRD results were also strongly associated with survival in AML, while the association in B-ALL was less evident. There was a moderate correlation between FC-MRD and Mol-MRD in B-ALL but not AML. The association of FC-MRD with relapse and survival was stronger in AML than in B-ALL. Overall, these findings suggest divergent utilities of FC-MRD in AML and B-ALL.
Industrial Scale Direct Liquefaction of E. globulus Biomass
Authors: Fernandes Irina , Correia Maria Joana Neiva , Condeço José , Cecílio Duarte M. , Bordado João , Mateus Margarida
Published: October 2023

This work presents the study of Eucalyptus globulus bark and sawdust direct liquefaction. Laboratory scale experiments were carried out to assess the impact of several variables on the reaction yield and the sugar content of the bio-oil. These variables were the biomass type and concentration, the solvent, and the reaction time. The results show that E. globulus sawdust presented the highest yields (>95%), but the highest sugar content after water extraction was obtained for E. globulus bark (~5.5% vs. 1.2% for sawdust). Simultaneously, industrial-scale tests were carried out at the ENERGREEN pilot plant using the same reaction variables, which resulted in reaction yields of nearly 100%. The reagents and raw materials used, as well as the products obtained (bio-oil, reaction condensates, polyols, and sugar phases) were characterized by elemental analysis, infrared spectroscopy, thermogravimetry, and high-performance liquid chromatography with mass spectrometry. The heating value of the bio-oils is higher than the original biomass (higher heating value of E. globulus sawdust bio-oil 29 MJ/kg vs. 19.5 MJ/kg of the original E. globulus sawdust). The analyses of the bio-oils allowed us to identify the presence of high-added-value compounds, such as levulinic acid and furfural. Finally, a study of the accelerated aging of liquefied biomass showed that the biofuel density increases from 1.35 to 1.44 kg/dm3 after 7 days of storage due to the occurrence of repolymerization reactions.
A New Ammonia Kinetic Model in Ru-Catalyzed Steam-Reforming Reaction Containing N2 in Natural Gas
Authors: Kim Chulmin , Lee Juhan , Lee Sangyong
Published: October 2023

Hydrogen for building fuel cells is primarily produced by natural-gas steam-reforming reactions. Pipeline-transported natural gas in Europe and North America used to contain about 1% to 5% N2, which reacts with H2 in steam-reforming reactions to form NH3. In the case of Ru, one of the catalysts used in natural-gas steam-reforming reactions, the activity of the NH3-formation reaction is higher than that of Ni and Rh catalysts. Reforming gas containing NH3 is known to poison Pt catalysts in Polymer Electrolyte Membrane Fuel Cells (PEMFCs) and also poison catalysts in preferential oxidation (PROX). In this study, Langmuir–Hinshelwood-based models of the NH3-formation reaction considering H2 and CO were proposed and compared with a simplified form of the Temkin–Pyzhev model for NH3-formation rate. The kinetic parameters of each model were optimized by performing multi-objective function optimization on the experimental results using a tube-type reactor and the numerical results of a plug-flow one-dimension simple SR (steam-reforming) reactor.
Astrocytes Undergo Metabolic Reprogramming in the Multiple Sclerosis Animal Model
Authors: das Neves Sofia Pereira , Sousa João Carlos , Magalhães Ricardo , Gao Fuying , Coppola Giovanni , Mériaux Sebatien , Boumezbeur Fawzi , Sousa Nuno , Cerqueira João José , Marques Fernanda
Published: October 2023

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system that presents a largely unknown etiopathology. The presence of reactive astrocytes in MS lesions has been described for a long time; however, the role that these cells play in the pathophysiology of MS is still not fully understood. Recently, we used an MS animal model to perform high-throughput sequencing of astrocytes’ transcriptome during disease progression. Our data show that astrocytes isolated from the cerebellum (a brain region typically affected in MS) showed a strong alteration in the genes that encode for proteins related to several metabolic pathways. Specifically, we found a significant increase in glycogen degradation, glycolytic, and TCA cycle enzymes. Together with these alterations, we detected an upregulation of genes that characterize “astrocyte reactivity”. Additionally, at each disease time point we also reconstructed the morphology of cerebellum astrocytes in non-induced controls and in EAE animals, near lesion regions and in the normal-appearing white mater (NAWM). We found that near lesions, astrocytes presented increased length and complexity compared to control astrocytes, while no significant alterations were observed in the NAWM. How these metabolic alterations are linked with disease progression is yet to be uncovered. Herein, we bring to the literature the hypothesis of performing metabolic reprogramming as a novel therapeutic approach in MS.
The Impact of the Nervous System on Arteries and the Heart: The Neuroimmune Cardiovascular Circuit Hypothesis
Authors: Mohanta Sarajo K. , Sun Ting , Lu Shu , Wang Zhihua , Zhang Xi , Yin Changjun , Weber Christian , Habenicht Andreas J. R.
Published: October 2023

Three systemic biological systems, i.e., the nervous, the immune, and the cardiovascular systems, form a mutually responsive and forward-acting tissue network to regulate acute and chronic cardiovascular function in health and disease. Two sub-circuits within the cardiovascular system have been described, the artery brain circuit (ABC) and the heart brain circuit (HBC), forming a large cardiovascular brain circuit (CBC). Likewise, the nervous system consists of the peripheral nervous system and the central nervous system with their functional distinct sensory and effector arms. Moreover, the immune system with its constituents, i.e., the innate and the adaptive immune systems, interact with the CBC and the nervous system at multiple levels. As understanding the structure and inner workings of the CBC gains momentum, it becomes evident that further research into the CBC may lead to unprecedented classes of therapies to treat cardiovascular diseases as multiple new biologically active molecules are being discovered that likely affect cardiovascular disease progression. Here, we weigh the merits of integrating these recent observations in cardiovascular neurobiology into previous views of cardiovascular disease pathogeneses. These considerations lead us to propose the Neuroimmune Cardiovascular Circuit Hypothesis.
Cell Cultures as a Versatile Tool in the Research and Treatment of Autoimmune Connective Tissue Diseases
Authors: Ejma-Multański Adam , Wajda Anna , Paradowska-Gorycka Agnieszka
Published: October 2023

Cell cultures are an important part of the research and treatment of autoimmune connective tissue diseases. By culturing the various cell types involved in ACTDs, researchers are able to broaden the knowledge about these diseases that, in the near future, may lead to finding cures. Fibroblast cultures and chondrocyte cultures allow scientists to study the behavior, physiology and intracellular interactions of these cells. This helps in understanding the underlying mechanisms of ACTDs, including inflammation, immune dysregulation and tissue damage. Through the analysis of gene expression patterns, surface proteins and cytokine profiles in peripheral blood mononuclear cell cultures and endothelial cell cultures researchers can identify potential biomarkers that can help in diagnosing, monitoring disease activity and predicting patient’s response to treatment. Moreover, cell culturing of mesenchymal stem cells and skin modelling in ACTD research and treatment help to evaluate the effects of potential drugs or therapeutics on specific cell types relevant to the disease. Culturing cells in 3D allows us to assess safety, efficacy and the mechanisms of action, thereby aiding in the screening of potential drug candidates and the development of novel therapies. Nowadays, personalized medicine is increasingly mentioned as a future way of dealing with complex diseases such as ACTD. By culturing cells from individual patients and studying patient-specific cells, researchers can gain insights into the unique characteristics of the patient’s disease, identify personalized treatment targets, and develop tailored therapeutic strategies for better outcomes. Cell culturing can help in the evaluation of the effects of these therapies on patient-specific cell populations, as well as in predicting overall treatment response. By analyzing changes in response or behavior of patient-derived cells to a treatment, researchers can assess the response effectiveness to specific therapies, thus enabling more informed treatment decisions. This literature review was created as a form of guidance for researchers and clinicians, and it was written with the use of the NCBI database.
The Effect of the Addition of Aluminum Nitride to the Composition of NiAl2O4 Ceramics on Hydrogenation Processes and the Increase in Resistance to Swelling and Degradation
Authors: Kozlovskiy Artem L.
Published: October 2023

This work examines the effects of the formation of impurity inclusions in the structure of NiAl2O4 ceramics when aluminum nitride is added to them and the occurrence of a reinforcement effect that prevents hydrogenation processes and the subsequent destruction of conductive and thermophysical characteristics. The appeal of ceramics possessing a spinel crystal structure lies in their potential use as ceramic fuel cells for both hydrogen generation and storage. Simultaneously, addressing the challenges related to ceramic degradation during hydrogenation, a critical aspect of hydrogen production, can enhance the efficiency of these ceramics while lowering electricity production costs. The selection of aluminum nitride as an additive for ceramic modification is based on its remarkable resistance to structural damage accumulation, its potential to enhance resistance to high-temperature degradation, and its ability to bolster strength properties. Moreover, an examination of the alterations in the strength characteristics of the examined samples subjected to hydrogenation reveals that the stability of two-phase ceramics is enhanced by more than three to five times compared to the initial ceramics (those without the addition of AlN). Additionally, it was noted that the most significant alterations in both structure and strength become apparent at irradiation fluences exceeding 1014 proton/cm2, where atomic displacements in the damaged ceramic layer reach over 5 dpa. During the evaluation of thermophysical properties, it was discerned that ceramics featuring an impurity phase in their composition exhibit the highest stability. These ceramics demonstrated a reduction in the thermal conductivity coefficient of less than 1% at the peak irradiation fluence.
Dyes Are the Rainbow of Our Health
Authors: Shammout Mohammad-Jamal A. , Alsaleh Majd M. , Natsheh Iyad Y. , Albadawi Duaa K. , Alkhawaldeh Ahmad K.
Published: October 2023

Natural dyes, obtained without the use of chemical treatment, are derived from naturally occurring sources, such as plants, animals, insects, and minerals. The usage of natural substances and their medicinal properties dates back to the origins of human civilization. The purpose of this review is to highlight the medicinal importance of selected natural colors, which sheds light on the critical role played by these dyes in the pharmaceutical industry. The objective is to showcase the health benefits of each color that can be obtained from nature for medicinal purposes based on their chemical structure. The review presents the reasons for utilizing natural resources in addressing various health issues, with a focus on three specific problems: microbial infections, cancer, and oxidative stress. Our review highlights the potential of natural resource structures, particularly anthocyanins, genipin, carotenoids, phycocyanin, and chlorophylls, in combating these ailments, emphasizing the need to explore their resources further for medicinal purposes. While most reviews provide a survey about colorful crude plant extracts in relation to one or a few categories of human health, our review focuses on the specific chromophore extracted not only from plants but also from any natural resource to provide a specific chromophore effect in a whole resource. The review highlights the significant role performed by organic pigments in the medicinal domain, with organic colorants acting as an essential element of the pharmaceutical sector’s weaponry. Hence, it is of paramount significance to actively promote and stress the adoptions of naturally existing chromophores in diverse everyday commodities, while simultaneously acknowledging and valuing their substantial importance and worth in the vast realm of the pharmaceutical industry.
Gas Chromatography–Mass Spectrometry Chemical Profiling and Radical Scavenging Potential of Sesquiterpene-Rich Essential Oil of Polygonum equisetiforme Sm.: In Silico Study on Hematopoietic Cell Kinase (Hck) and Human Peroxiredoxin 5
Authors: Abd-ElGawad Ahmed M. , Ahmed Rania F. , Essa Ahmed F. , Gendy Abd El-Nasser G. El , El-Newary Samah A. , Elshamy Abdelsamed I. , Sarker Tushar C. , El-Amier Yasser A.
Published: October 2023

Essential oils (EOs) are advised by traditional medical systems for the treatment of a variety of disorders worldwide. In many ancient medical systems around the world, Polygonum herbs have been employed as remedies including P. equisetiforme Sm. The EO profile of P. equisetiforme and its bioactivities have yet to be discussed in depth. As a result, the current study aims to investigate the chemical profile and free radical scavenging capacity of P. equisetiforme EO. Hydrodistillation was used to obtain the EO from P. equisetiforme, and gas chromatography–mass spectrometry (GC-MS) was used for analysis. A total of forty-three compounds, including terpenes and sesquiterpenes as the main components (76.13% and 69.06%, respectively), were identified in the oil using the GC-MS analysis. The main constituents of the oil were hexahydrofarnesyl acetone (29.45%), 7-epi-selinene (14.45%), isospathulenol (8.35%), and n-docosane (6.79%). The chemosystematic significance of the plant was established via multivariate assessing, comprising principal component analysis (PCA), hierarchical clustering, and constellation plot, of the EO principal components of the various Polygonum plants. The P. equisetiforme exhibited different associations with the studied Polygonum spp. Then, the scavenging of the free radicals 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was used to evaluate the radical scavenging abilities of EO compared with those of vitamin C, a reference antioxidant. P. equisetiforme EO exhibited the scavenging capacity of the DPPH and the ABTS free radical with respective IC50 values of 470.01 and 113.74 mg L−1 compared with vitamin C, and with IC50 values of 39.06 and 26.09 mg L−1, respectively. The in silico studies revealed that the oxygenated sesquiterpenes, especially ar-turmerone, hexahydrofarnesyl acetone, and 5E,9E-farnesyl acetone, exhibited the best fitting with hematopoietic cell kinase (Hck) and human Peroxiredoxin 5 proteins with ΔG values of −6.14 and −4.93, −6.83 and −5.34, and −7.08 and −5.47 kcal/mol, respectively. The major components’ combined or individual effects may be responsible for the antioxidant properties. Therefore, additional extensive studies are advised to characterize the essential compounds as radical scavenger agents, either individually or in combination.
Synthesis and Characterization of a Self-Crosslinked Organic Copolymer Kappa-Carrageenan/Polyacrylamide/Cetrimide (κ-CAR/PAAm/CI) Hydrogel with Antimicrobial and Anti-Inflammatory Activities for Wound Healing
Authors: Agili Fatimah A. , Mohamed Sahera F.
Published: October 2023

The current study aimed to produce a material that has dual effects of healing and anti-inflammatory activity. For this purpose, a κ-carrageenan/polyacrylamide film loaded with cetrimide (κ-CAR/PAAm/CI) was developed using the manual casting technique. Definite concentrations of κ-CAR and AAm were heated at 80 °C for 2 h, and CI and glycerol were added. The solution was cast without using an initiator or crosslinker. The reaction of the sulfonic acid group -SO3H of κ-CAR with the –CONH2 group of PAAm lead to the formation of a sulfonamide (–SO2NH–) group. The characteristics of the produced films were investigated based on FT-IR, TGA, the contact angle, and mechanical properties. An improvement in the thermal stability of the κ-CAR/PAAm/CI2 film containing 1.5% CI was achieved, compared to the film with 0.5% CI (κ-CAR/PAAm/CI1). The contact angle measurement proved that the films were hydrophobic, enhanced by increasing the CI content. The tensile strength and elongation percent values are considered adequate for materials used in wound care. The κ-CAR/PAAm/CI2 (1.5% CI) film showed superior antimicrobial activity against P. aeruginosa, moderate activity against S. aureus, and low activity against E. coli. The κ-CAR/PAAm/CI2 film effectively inhibited heat-induced hemolysis and showed wound contraction activity at a level of 100% after 19 days of excision wound treatment. The prepared films may offer a promising approach for the development of effective wound dressings.
Delipidation of Chicken Feathers by Lipolytic Bacillus Species Isolated from River-Borne Sediments
Authors: Shiri Tariro , Nnolim Nonso E. , Nwodo Uchechukwu U.
Published: October 2023

Though the keratin content of chicken feathers is being explored for many potential uses, the crude lipid content of the biomass significantly hinders the valorization processes. Therefore, this study explored the potential of bacteria isolated from sediment for lipolytic properties. Sediment-associated strains were evaluated for lipolytic activity on tween 80–peptone agar. The best lipolytic bacterium was used to break down the lipid content of chicken feathers. The results showed that out of six bacterial strains with variable lipolytic activity, strain TTs1 showed the largest zone of precipitate around the colony, which is why it was selected and identified as Bacillus sp. TTs1. The maximum lipase production of 1530.5 U/mL by strain TTs1 was achieved at 96 h post-fermentation, with optimal process conditions of initial pH (10), incubation temperature (45 °C), agitation speed (140 rpm), inoculum size (2% v/v) and tween 80 (10% v/v). The total free fatty acid (0.58%) was liberated from chicken feathers at a concentration of 6% (w/v). Crude fat extraction from both untreated and TTs1-pretreated chicken feathers showed fat contents of 2.1 ± 0.42% and 0.92 ± 0.13%, respectively. The findings of this study highlight the biotechnological relevance of strain TTs1 in lipase production and the sustainable delipidation of lipid-rich bioresources.
A Review on Tribocorrosion Behavior of Aluminum Alloys: From Fundamental Mechanisms to Alloy Design Strategies
Authors: Zhang Zhengyu , Dandu Raja Shekar Bhupal , Klu Edwin Eyram , Cai Wenjun
Published: October 2023

Tribocorrosion, a research field that has been evolving for decades, has gained renewed attention in recent years, driven by increased demand for wear- and corrosion-resistant materials from biomedical implants, nuclear power generation, advanced manufacturing, batteries, marine and offshore industries, etc. In the United States, wear and corrosion are estimated to cost nearly USD 300 billion per year. Among various important structural materials, passive metals such as aluminum alloys are most vulnerable to tribocorrosion due to the wear-accelerated corrosion as a result of passive film removal. Thus, designing aluminum alloys with better tribocorrosion performance is of both scientific and practical importance. This article reviews five decades of research on the tribocorrosion of aluminum alloys, from experimental to computational studies. Special focus is placed on two aspects: (1) The effects of alloying and grain size on the fundamental wear, corrosion, and tribocorrosion mechanisms; and (2) Alloy design strategies to improve the tribocorrosion resistance of aluminum alloys. Finally, the paper sheds light on the current challenges faced and outlines a few future research directions in the field of tribocorrosion of aluminum alloys.
Biodegradable Conductive Layers Based on a Biopolymer Polyhydroxybutyrate/Polyhydroxyvalerate and Graphene Nanoplatelets Deposited by Spray-Coating Technique
Authors: Lepak-Kuc Sandra , Wójkowska Katarzyna , Biernacka Dorota , Kądziela Aleksandra , Murawski Tomasz Tadeusz , Janczak Daniel , Jakubowska Małgorzata
Published: October 2023

In light of the growing concern for environmental protection and the alarming amount of waste produced due to hygiene regulations, this study suggests a biodegradable and eco-friendly solution that could make a significant contribution to the preservation of our planet. The developed solution was based on a polyhydroxybutyrate/polyhydroxyvalerate biopolymer, which has been tested regarding its physicochemical parameters and possible use in printed electrically conductive structures. Graphene nanoplatelets have been used as the conductive functional phase, due to literature reports of their potential use in biomedical applications and due to the potential of providing cytocompatibility in electrical structures by carbon nanomaterials. Prepared composites have been spray-coated onto PET film and paper substrates and then subjected to electrical, adhesion and optical measurements. In order to establish the conductivity of the developed composite, its resistance, layer thickness and surface topography were measured. Optical parameters have been specified using scanning electron microscopy (SEM) imaging and spectrophotometry. The conducted research opens a wide path for the use of the polyhydroxybutyrate/polyhydroxyvalerate biopolymer with graphene nanoplatelets in biomedical applications, ensuring good conductivity, biocompatibility and stability.
SigML++: Supervised Log Anomaly with Probabilistic Polynomial Approximation
Authors: Trivedi Devharsh , Boudguiga Aymen , Kaaniche Nesrine , Triandopoulos Nikos
Published: October 2023

Security log collection and storage are essential for organizations worldwide. Log analysis can help recognize probable security breaches and is often required by law. However, many organizations commission log management to Cloud Service Providers (CSPs), where the logs are collected, processed, and stored. Existing methods for log anomaly detection rely on unencrypted (plaintext) data, which can be a security risk. Logs often contain sensitive information about an organization or its customers. A more secure approach is always to keep logs encrypted (ciphertext). This paper presents “SigML++”, an extension of “SigML” for supervised log anomaly detection on encrypted data. SigML++ uses Fully Homomorphic Encryption (FHE) according to the Cheon–Kim–Kim–Song (CKKS) scheme to encrypt the logs and then uses an Artificial Neural Network (ANN) to approximate the sigmoid (σ(x)) activation function probabilistically for the intervals [−10,10] and [−50,50]. This allows SigML++ to perform log anomaly detection without decrypting the logs. Experiments show that SigML++ can achieve better low-order polynomial approximations for Logistic Regression (LR) and Support Vector Machine (SVM) than existing methods. This makes SigML++ a promising new approach for secure log anomaly detection.
Investigation of the Processes of Structure Formation during Explosion Welding of Copper and Molybdenum
Authors: Noskov Fedor M. , Kveglis Lyudmila I. , Mali Vyacheslav I. , Esikov Maksim A. , Sakenova Rimma Y.
Published: October 2023

This article examines the processes of structure formation occurring during joint plastic deformation by the explosion of copper and molybdenum. These components are dissimilar metals with very limited mutual solubility under normal conditions, and the circumstances allowing for their interaction, as well as the products of the mechanochemical reactions of such interactions, have not been sufficiently studied and require new approaches. A cluster approach was used to describe the processes of structure formation, which describes phase formation as the process transitioning of the polyhedron of the initial phase into the polyhedron of the final phase. This work shows that under the conditions under consideration, not only is the formation of solid solutions in the contact zone with smooth concentration transitions from one component to another possible, but also the formation of new structural states, which can be represented as localized icosahedral atomic configurations (amorphous metal clusters). Such a structure is capable of locally strengthening the composite, which is confirmed by microhardness studies.
Substituent Effects in Weak Charge-Transfer Cocrystals of Benzene Derivatives with Classical TCNQ Acceptors: Experimental and Theoretical Study
Authors: Latypov Shamil , Fedonin Anton , Ivshin Kamil , Zinnatullin Ruzal , Metlushka Kirill , Kataeva Olga
Published: October 2023

A series of xylene charge-transfer complexes with fluorine-substituted tetracyanoquinodimethane (TCNQ) acceptors were studied experimentally and theoretically in order to reveal the role of various intermolecular interactions on stoichiometry and the crystal structure. It was shown that o-xylene face-to-face donor–donor interactions became significant enough to result in the formation of 2:1 cocrystals with F1TCNQ and F4TCNQ irrespective of growth conditions. The supramolecular arrangement in these cocrystals is mainly determined by the number of fluorine atoms in the acceptor. Comparative DFT and MP2 calculations of the pairwise intermolecular interactions revealed the overestimation of the dispersion energy for these systems by the DFT-wB97XD approach.
Ethoxylate Polymer-Based 96-Well Screen for Protein Crystallization
Authors: Demmer Ulrike , Lemaire Olivier N. , Belhamri Mélissa , Ermler Ulrich
Published: October 2023

Crystallization is the limiting step in X-ray structure determination of biological macromolecules. As crystallization experiments can be largely automatized, the diversity of precipitant solutions is often the determinant factor to obtain crystals of high quality. Here, we introduce a 96-well screening kit of crystallization conditions, centered on three ethoxylate-based organic polymers as precipitants and various additional compounds to promote crystal formation. This crystallization screen was tested on various non-standard proteins from bacteria and archaea. Structure determination succeeded for seven out of thirteen targets based on crystals that frequently diffracted to a higher resolution than those obtained with commercially available screening kits. Crystallization hits were rarely similar among the three ethoxylate-based organic polymers and, in comparison, with already available crystallization screens. Hence, the presented crystallization screen is an efficient tool to complement other screens and increase the likelihood of growing crystals suitable for X-ray structure determination.
Evaluation of Anti-Mullerian Hormone Levels, Antral Follicle Counts, and Mean Ovarian Volumes in Chemotherapy-Induced Amenorrhea among Breast Cancer Patients: A Prospective Clinical Study
Authors: Ünal Çağlar , Ordu Çetin , Özmen Tolga , İlgun Ahmet Serkan , Çelebi Filiz , Baysal Bülent , Özkurt Enver , Duymaz Tomris , Erdoğan İyigün Zeynep , Kurt Sevgi , Öztürk Mehmet Alper , Pilancı Kezban Nur , Alço Gül , Yararbaş Kanay , Kayan Tapan Tuba , Güven Deniz Can , Soybir Gürsel , Özmen Vahit
Published: October 2023

Estradiol (E2), a follicle-stimulating hormone (FSH), AMH, and inhibin B levels, along with AFC and MOV, are used to determine ovarian reserve in pre-menopausal women. Studies have shown that AMH levels are more sensitive than those of E2, FSH, and inhibin B and that AFC and MOV can be used to evaluate ovarian reserve. AMH, AFC, and MOV measurements were performed before and after adjuvant SC in 3-month periods for one year. Patients were classified as experiencing chemotherapy-induced amenorrhea (CIA) if they did not have menstrual cycles for a period of six months or longer following the conclusion of their chemotherapy treatment. We aimed to evaluate the factors affecting chemotherapy-induced amenorrhea in breast cancer patients treated with adjuvant chemotherapy and the performance of baseline measurements of AMH, AFC, and MOV to predict chemotherapy-induced amenorrhea. The effects of different chemotherapy regimens on the AMH level, AFC, and MOV in CIA patients were investigated. Seventy-one patients were eligible for this study, and the median age was 38 years (range: 23–45). The median follow-up was 37 months (range: 20–51), and CIA developed in 62% of the patients. The AMH level and AFC were significantly decreased one year after SC (p < 0.0001), whereas MOV was not (p = 0.507). AMH levels before chemotherapy (median: 1.520 vs. 0.755, p = 0.001) and at the end of the first year (median: 0.073 vs. 0.010, p = 0.030) and pre-treatment AFC (median: 12 vs. 4.50, p = 0.026) were lower in patients with CIA compared to those without CIA. The AMH levels before SC were the most valuable and earliest factor for predicting CIA development. In addition, there was no difference between the chemotherapy regimens (including or not including taxane) in terms of CIA development.
Local Control Following Stereotactic Body Radiation Therapy for Liver Oligometastases: Lessons from a Quarter Century
Authors: Mheid Sara , Allen Stefan , Ng Sylvia S. W. , Hall William A. , Sanford Nina N. , Aguilera Todd A. , Elamir Ahmed M. , Bahij Rana , Intven Martijn P. W. , Radhakrishna Ganesh , Mohamad Issa , De Leon Jeremy , Tan Hendrick , Lewis Shirley , Gani Cihan , Stanecu Teo , Dell’Acqua Veronica , Hosni Ali
Published: October 2023

The utilization of stereotactic body radiation therapy for the treatment of liver metastasis has been widely studied and has demonstrated favorable local control outcomes. However, several predictive factors play a crucial role in the efficacy of stereotactic body radiation therapy, such as the number and size (volume) of metastatic liver lesions, the primary tumor site (histology), molecular biomarkers (e.g., KRAS and TP53 mutation), the use of systemic therapy prior to SBRT, the radiation dose, and the use of advanced technology and organ motion management during SBRT. These prognostic factors need to be considered when clinical trials are designed to evaluate the efficacy of SBRT for liver metastases.
Dynamic Graph Clustering Learning for Unsupervised Diabetic Retinopathy Classification
Authors: Yu Chenglin , Pei Hailong
Published: October 2023

Diabetic retinopathy (DR) is a common complication of diabetes, which can lead to vision loss. Early diagnosis is crucial to prevent the progression of DR. In recent years, deep learning approaches have shown promising results in the development of an intelligent and efficient system for DR classification. However, one major drawback is the need for expert-annotated datasets, which are both time-consuming and costly. To address these challenges, this paper proposes a novel dynamic graph clustering learning (DGCL) method for unsupervised classification of DR, which innovatively deploys the Euclidean and topological features from fundus images for dynamic clustering. Firstly, a multi-structural feature fusion (MFF) module extracts features from the structure of the fundus image and captures topological relationships among multiple samples, generating a fused representation. Secondly, another consistency smoothing clustering (CSC) module combines network updates and deep clustering to ensure stability and smooth performance improvement during model convergence, optimizing the clustering process by iteratively updating the network and refining the clustering results. Lastly, dynamic memory storage is utilized to track and store important information from previous iterations, enhancing the training stability and convergence. During validation, the experimental results with public datasets demonstrated the superiority of our proposed DGCL network.
More Than a Decade of GeneXpert® Mycobacterium tuberculosis/Rifampicin (Ultra) Testing in South Africa: Laboratory Insights from Twenty-Three Million Tests
Authors: da Silva Manuel Pedro , Cassim Naseem , Ndlovu Silence , Marokane Puleng Shiela , Radebe Mbuti , Shapiro Anne , Scott Lesley Erica , Stevens Wendy Susan
Published: October 2023

This study seeks to describe the rollout and current state of South Africa’s GeneXpert molecular diagnostic program for tuberculosis (TB). Xpert MTB/RIF was introduced in 2011 with a subsequent expansion to include extra-pulmonary and paediatric testing, followed by Xpert MTB/RIF Ultra in 2017. Through a centralised laboratory information system and the use of a standardised platform for more than a decade, over 23 million tests were analysed, describing the numbers tested, Mycobacterium tuberculosis complex detection, rifampin resistance, and the unsuccessful test rates. The stratification by province, specimen type, age, and sex identified significant heterogeneity across the program and highlighted testing gaps for men, low detection yield for paediatric pulmonary TB, and the effects of inadequate specimen quality on the detection rate. The insights gained from these data can aid in the monitoring of interventions in support of the national TB program beyond laboratory operational aspects.
Proximal Caries Detection Using Short-Wave Infrared Transillumination at Wavelengths of 1050, 1200 and 1300 nm in Permanent Posterior Human Teeth
Authors: Heck Katrin , Kunzelmann Karl-Heinz , Walter Elias , Kaisarly Dalia , Hoffmann Lea , Litzenburger Friederike
Published: October 2023

This in vitro study aimed to investigate the diagnostic potential of short-wave infrared transillumination (SWIRT) at 1050, 1200 and 1300 nm for the detection of proximal caries in molars and premolars. It was compared to the diagnostic performance of bitewing radiography (BWR) and micro-computed tomography (µCT) as the reference standard. 250 sound or decayed proximal surfaces of permanent posterior extracted teeth were examined using (1) SWIRT at 1050, 1200 and 1300 nm with two camera systems of different resolutions, (2) BWR and (3) µCT. Thresholds were defined for both test methods and the reference standard for caries in general, enamel caries and dentin caries. All images were assessed by two examiners twice, at an interval of two weeks. SWIRT at wavelengths of 1050, 1200 and 1300 nm achieved sensitivity values more than 2.5 times higher than BWR (enamel caries 3.2–4.4 times; dentin caries 3.25–4.25 times) for the detection of proximal caries. Sensitivity values of SWIRT improved with the higher wavelength. No significant difference was found in diagnostic quality between the two camera systems. SWIRT at 1300 nm imaged proximal enamel caries with the highest accuracy, while the physical optimum for transillumination in dentin was located at a lower wavelength (<1000 nm).
Prognostic Significance of Programmed Cell Death Ligand 1 Expression in High-Grade Serous Ovarian Carcinoma: A Systematic Review and Meta-Analysis
Authors: Kang Jeongwan , Han Kang Min , Jung Hera , Kim Hyunchul
Published: October 2023

(1) Background: High-grade serous ovarian carcinoma (HGSOC) is an aggressive subtype of ovarian cancer. Recent advances have introduced prognostic markers and targeted therapies. Programmed cell death ligand 1 (PD-L1) has emerged as a potential biomarker for HGSOC, with implications for prognosis and targeted therapy eligibility; (2) Methods: A literature search was conducted on major databases, and extracted data were categorized and pooled. Subgroup analysis was performed for studies with high heterogeneity. (3) Results: Data from 18 eligible studies were categorized and pooled based on PD-L1 scoring methods, survival analysis types, and endpoints. The result showed an association between high PD-L1 expression and a favorable prognosis in progression-free survival (HR = 0.53, 95% CI = 0.35–0.78, p = 0.0015). Subgroup analyses showed similar associations in subgroups of neoadjuvant chemotherapy patients (HR = 0.6, 95% CI = 0.4–0.88, p = 0.009) and European studies (HR = 0.59, 95% CI = 0.42–0.82, p = 0.0017). In addition, subgroup analyses using data from studies using FDA-approved PD-L1 antibodies suggested a significant association between favorable prognosis and high PD-L1 expression in a subgroup including high and low stage data in overall survival data (HR = 0.46, 95% CI = 0.3–0.73, p = 0.0009). (4) Conclusions: This meta-analysis revealed a potential association between high PD-L1 expression and favorable prognosis. However, caution is warranted due to several limitations. Validation via large-scale studies, with mRNA analysis, whole tissue sections, and assessments using FDA-approved antibodies is needed.
Correlation of Perfusion Metrics with Ki-67 Proliferation Index and Axillary Involvement as a Prognostic Marker in Breast Carcinoma Cases: A Dynamic Contrast-Enhanced Perfusion MRI Study
Authors: Uncu Ulas Yalim , Aydin Aksu Sibel
Published: October 2023

Our study aims to reveal clinically helpful prognostic markers using quantitative radiologic data from perfusion magnetic resonance imaging for patients with locally advanced carcinoma, using the Ki-67 index as a surrogate. Patients who received a breast cancer diagnosis and had undergone dynamic contrast-enhanced magnetic resonance imaging of the breast for pretreatment evaluation and follow-up were searched retrospectively. We evaluated the MRI studies for perfusion parameters and various categories and compared them to the Ki-67 index. Axillary involvement was categorized as low (N0–N1) or high (N2–N3) according to clinical stage. A total sum of 60 patients’ data was included in this study. Perfusion parameters and Ki-67 showed a significant correlation with the transfer constant (Ktrans) (ρ = 0.554 p = 0.00), reverse transfer constant (Kep) (ρ = 0.454 p = 0.00), and initial area under the gadolinium curve (IAUGC) (ρ = 0.619 p = 0.00). The IAUGC was also significantly different between axillary stage groups (Z = 2.478 p = 0.013). Outside of our primary hypothesis, associations between axillary stage and contrast enhancement (x2 = 8.023 p = 0.046) and filling patterns (x2 = 8.751 p = 0.013) were detected. In conclusion, these parameters are potential prognostic markers in patients with moderate Ki-67 indices, such as those in our study group. The relationship between axillary status and perfusion parameters also has the potential to determine patients who would benefit from limited axillary dissection.
Quantifying Soil Complexity Using Fisher Shannon Method on 3D X-ray Computed Tomography Scans
Authors: Aguiar Domingos , Menezes Rômulo Simões Cezar , Antonino Antonio Celso Dantas , Stosic Tatijana , Tarquis Ana M. , Stosic Borko
Published: October 2023

The conversion of native forest into agricultural land, which is common in many parts of the world, poses important questions regarding soil degradation, demanding further efforts to better understand the effect of land use change on soil functions. With the advent of 3D computed tomography techniques and computing power, new methods are becoming available to address this question. In this direction, in the current work we implement a modification of the Fisher–Shannon method, borrowed from information theory, to quantify the complexity of twelve 3D CT soil samples from a sugarcane plantation and twelve samples from a nearby native Atlantic forest in northeastern Brazil. The distinction found between the samples from the sugar plantation and the Atlantic forest site is quite pronounced. The results at the level of 91.7% accuracy were obtained considering the complexity in the Fisher–Shannon plane. Atlantic forest samples are found to be generally more complex than those from the sugar plantation.
Variable-Length Resolvability for General Sources and Channels
Authors: Yagi Hideki , Han Te Sun
Published: October 2023

We introduce the problem of variable-length (VL) source resolvability, in which a given target probability distribution is approximated by encoding a VL uniform random number, and the asymptotically minimum average length rate of the uniform random number, called the VL resolvability, is investigated. We first analyze the VL resolvability with the variational distance as an approximation measure. Next, we investigate the case under the divergence as an approximation measure. When the asymptotically exact approximation is required, it is shown that the resolvability under two kinds of approximation measures coincides. We then extend the analysis to the case of channel resolvability, where the target distribution is the output distribution via a general channel due to a fixed general source as an input. The obtained characterization of channel resolvability is fully general in the sense that, when the channel is just an identity mapping, it reduces to general formulas for source resolvability. We also analyze the second-order VL resolvability.