bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of CD4 Binding on Conformational Dynamics, Molecular Motions, and Thermodynamics of HIV-1 gp120
Authors: Li Yi , Deng Lei , Yang Li-Quan , Sang Peng , Liu Shu-Qun
Published: January 2019

Human immunodeficiency virus type-1 (HIV-1) infection is triggered by its envelope (Env) glycoprotein gp120 binding to the host-cell receptor CD4. Although structures of Env/gp120 in the liganded state are known, detailed information about dynamics of the liganded gp120 has remained elusive. Two structural models, the CD4-free gp120 and the gp120-CD4 complex, were subjected to µs-scale multiple-replica molecular dynamics (MD) simulations to probe the effects of CD4 binding on the conformational dynamics, molecular motions, and thermodynamics of gp120. Comparative analyses of MD trajectories in terms of structural deviation and conformational flexibility reveal that CD4 binding effectively suppresses the overall conformational fluctuations of gp120. Despite the largest fluctuation amplitude of the V1/V2 region in both forms of gp120, the presence of CD4 prevents it from approaching the gp120 core. Comparison of the constructed free energy landscapes (FELs) shows that CD4 binding reduces the conformational entropy and conformational diversity while enhancing the stability of gp120. Further comparison of the representative structures extracted from free energy basins/minima of FELs reveals that CD4 binding weakens the reorientation ability of V1/V2 and hence hinders gp120 from transitioning out of the liganded state to the unliganded state. Therefore, locking gp120 conformation via restraining V1/V2 reorientation with small molecules seems to be a promising strategy to control HIV-1 infection. Our computer simulation results support the conformational selection mechanism for CD4 binding to gp120 and facilitate the understanding of HIV-1 immune evasion mechanisms.
Ciprofloxacin Enhances the Chemosensitivity of Cancer Cells to ABCB1 Substrates
Authors: Gupta Pranav , Gao Hai-Ling , Ashar Yunali V. , Karadkhelkar Nishant M. , Yoganathan Sabesan , Chen Zhe-Sheng
Published: January 2019

ABCB1 is one of the major drug efflux transporters that is known to cause multidrug resistance (MDR) in cancer patients receiving chemotherapy for the treatment of solid tumors and hematological malignancies. Inhibition of ABCB1 efflux function is important for maintaining the intracellular concentration of chemotherapeutic drugs. Here, we evaluated ciprofloxacin for its ability to reverse MDR caused by the overexpression of ABCB1. Cytotoxicity of ciprofloxacin was determined by the MTT assay. The chemosensitizing effects of ciprofloxacin were determined in combination with ABCB1 substrates. The intracellular accumulation and efflux of ABCB1 substrates was measured by a scintillation counter, and protein expression was determined by the Western blotting. Vanadate-sensitive ATPase assay was performed to determine the effect of ciprofloxacin on the ATPase activity of ABCB1, and docking analysis was done to determine the interaction of ciprofloxacin with ABCB1. Ciprofloxacin significantly potentiated the cytotoxic effects of ABCB1 substrates in ABCB1-overexpressing cells. Furthermore, ciprofloxacin increased the intracellular accumulation and decreased the efflux of [3H]-paclitaxel without altering the expression of ABCB1. Ciprofloxacin stimulated the ATPase activity of ABCB1 in a concentration-dependent manner. Our findings showed that ciprofloxacin potently inhibits the ABCB1 efflux function and it has potential to be developed as a combination anticancer therapy.
Sevoflurane, Propofol and Carvedilol Block Myocardial Protection by Limb Remote Ischemic Preconditioning
Authors: Cho Youn Joung , Nam Karam , Kim Tae Kyong , Choi Seong Woo , Kim Sung Joon , Hausenloy Derek J , Jeon Yunseok
Published: January 2019

The effects of remote ischemic preconditioning (RIPC) in cardiac surgery have been inconsistent. We investigated whether anesthesia or beta-blockers interfere with RIPC cardioprotection. Fifty patients undergoing cardiac surgery were randomized to receive limb RIPC (four cycles of 5-min of upper arm cuff inflation/deflation) in the awake state (no-anesthesia; n = 17), or under sevoflurane (n = 17) or propofol (n = 16) anesthesia. In a separate crossover study, 11 healthy volunteers received either carvedilol or no medication prior to RIPC. Plasma dialysates were obtained and perfused through an isolated male Sprague–Dawley rat heart subjected to 30-min ischemia/60-min reperfusion, following which myocardial infarct (MI) size was determined. In the cardiac surgery study, pre-RIPC MI sizes were similar among the groups (39.7 ± 4.5% no-anesthesia, 38.9 ± 5.3% sevoflurane, and 38.6 ± 3.6% propofol). However, post-RIPC MI size was reduced in the no-anesthesia group (27.5 ± 8.0%; p < 0.001), but not in the anesthesia groups (35.7 ± 6.9% sevoflurane and 35.8 ± 5.8% propofol). In the healthy volunteer study, there was a reduction in MI size with RIPC in the no-carvedilol group (41.7 ± 4.3% to 30.6 ± 8.5%; p < 0.0001), but not in the carvedilol group (41.0 ± 4.0% to 39.6 ± 5.6%; p = 0.452). We found that the cardioprotective effects of limb RIPC were abolished under propofol or sevoflurane anesthesia and in the presence of carvedilol therapy.
Platelet-Rich Plasma in Treatment of Temporomandibular Joint Dysfunctions: Narrative Review
Authors: Zotti Francesca , Albanese Massimo , Rodella Luigi Fabrizio , Nocini Pier Francesco
Published: January 2019

Background: The aims of this narrative review were to examine up-to-date literature in order to evaluate the effectiveness of arthrocentesis or injections with platelet-rich plasma in temporomandibular affections and to compare them to arthrocentesis alone or with hyaluronic acid (HA) or to hyaluronic acid injections. Methods: The search of international literature was made on the PMC, PubMed and Cochrane databases, including all full-length text of studies on humans focused on osteoarthritis and disc displacements and their treatment with platelet-rich plasma arthrocentesis or injections. All design studies were included in the review and they were examined for three different outcomes: pain, joint sound and mandibular motion. English papers were only selected. Results: Even though the low number of studies in this field, arthrocentesis with platelet-rich plasma and platelet-rich plasma injections in temporomandibular disorders’ management were found to be effective in reducing pain and joint sound as well as in improving mandibular motion in a maximum follow-up of 24 months. Conclusion: Comparison to arthrocentesis alone or to HA use in arthrocentesis or by injections provided encouraging results in terms of the effectiveness of platelet-rich plasma use.
Astrocyte Signaling in the Neurovascular Unit After Central Nervous System Injury
Authors: Huang Lena , Nakamura Yoshihiko , Lo Eng H. , Hayakawa Kazuhide
Published: January 2019

Astrocytes comprise the major non-neuronal cell population in the mammalian neurovascular unit. Traditionally, astrocytes are known to play broad roles in central nervous system (CNS) homeostasis, including the management of extracellular ion balance and pH, regulation of neurotransmission, and control of cerebral blood flow and metabolism. After CNS injury, cell–cell signaling between neuronal, glial, and vascular cells contribute to repair and recovery in the neurovascular unit. In this mini-review, we propose the idea that astrocytes play a central role in organizing these signals. During CNS recovery, reactive astrocytes communicate with almost all CNS cells and peripheral progenitors, resulting in the promotion of neurogenesis and angiogenesis, regulation of inflammatory response, and modulation of stem/progenitor response. Reciprocally, changes in neurons and vascular components of the remodeling brain should also influence astrocyte signaling. Therefore, understanding the complex and interdependent signaling pathways of reactive astrocytes after CNS injury may reveal fundamental mechanisms and targets for re-integrating the neurovascular unit and augmenting brain recovery.
Quantum-Behaved Particle Swarm Optimization with Weighted Mean Personal Best Position and Adaptive Local Attractor
Authors: Chen Shouwen
Published: January 2019

Motivated by concepts in quantum mechanics and particle swarm optimization (PSO), quantum-behaved particle swarm optimization (QPSO) was proposed as a variant of PSO with better global search ability. In this paper, a QPSO with weighted mean personal best position and adaptive local attractor (ALA-QPSO) is proposed to simultaneously enhance the search performance of QPSO and acquire good global optimal ability. In ALA-QPSO, the weighted mean personal best position is obtained by distinguishing the difference of the effect of the particles with different fitness, and the adaptive local attractor is calculated using the sum of squares of deviations of the particles’ fitness values as the coefficient of the linear combination of the particle best known position and the entire swarm’s best known position. The proposed ALA-QPSO algorithm is tested on twelve benchmark functions, and compared with the basic Artificial Bee Colony and the other four QPSO variants. Experimental results show that ALA-QPSO performs better than those compared method in all of the benchmark functions in terms of better global search capability and faster convergence rate.
A Signal Period Detection Algorithm Based on Morphological Self-Complementary Top-Hat Transform and AMDF
Authors: Han Zhao , Wang Xiaoli
Published: January 2019

Period detection technology for weak characteristic signals is very important in the fields of speech signal processing, mechanical engineering, etc. Average magnitude difference function (AMDF) is a widely used method to extract the period of periodic signal for its low computational complexity and high accuracy. However, this method has low detection accuracy when the background noise is strong. In order to improve this method, this paper proposes a new method of period detection of the signal with single period based on the morphological self-complementary Top-Hat (STH) transform and AMDF. Firstly, the signal is de-noised by the morphological self-complementary Top-Hat transform. Secondly, the average magnitude difference function of the noise reduction sequence is calculated, and the falling trend is suppressed. Finally, a calculating adaptive threshold is used to extract the peaks at the position equal to the period of periodic signal. The experimental results show that the accuracy of periodic extraction of AMDF after Top-Hat filtering is better than that of AMDF directly. In summary, the proposed method is reliable and stable for detecting the periodic signal with weak characteristics.
Genome-Wide Transcriptome Profiling Reveals Genes Associated with Meiotic Drive System of Aedes aegypti
Authors: Shin Dongyoung , Behura Susanta K. , Severson David W.
Published: January 2019

Aedes aegypti is an important mosquito vector of several arboviruses, including dengue, yellow fever, Zika, and Chikungunya, which cause significant human morbidity and mortality globally. In certain populations of this mosquito, a native meiotic drive system causes abnormal spermatogenesis that results in highly male-biased progenies from some matings. Although the basic genetics and cytogenetics of the drive mechanism were elucidated, very little is known on a transcriptome level about how the meiotic drive phenotype is expressed in individual males. To address this question, we conducted a whole-genome microarray expression study of testes from a meiotic-drive-carrying strain (T37) in comparison with testes from a non-drive-carrying strain (RED). Based on bioinformatics analyses of the microarray data, we identified 209 genes associated with the meiotic drive phenotype that were significantly differentially expressed between the two strains. K-means cluster analysis revealed nine clusters, in which genes upregulated in T37 testes were assigned to five clusters and genes downregulated in T37 testes were assigned to four clusters. Our data further revealed that genes related to protein translation, phosphorylation, and binding, as well as to G-protein-coupled receptor (GPCR) and peptidase activities, are differentially upregulated in testes from males with the meiotic drive genotype. Based on pathway analysis of these differentially expressed genes, it was observed that the glycosylphosphatidylinositol (GPI)-anchor biosynthesis pathway may play a role in the meiotic drive system. Overall, this investigation enhances our understanding of whole-genome gene expression associated with the meiotic drive system in Ae. aegypti.
Exposure to Herbicides Prime P450-Mediated Detoxification of Helicoverpa armigera against Insecticide and Fungal Toxin
Authors: Sun Zhongxiang , Xu Cuicui , Chen Shi , Shi Qi , Wang Huanhuan , Wang Rumeng , Song Yuanyuan , Zeng Rensen
Published: January 2019

With the long-term and large-scale use, herbicides have been well known to influence tritrophic interactions, particularly natural enemies of pests in agro-ecosystems. On the other hand, herbivorous insects, especially the generalist pests, have developed antagonistic interaction to different insecticides, toxic plant secondary metabolites, and even heavy metals. However, whether exposure to herbicides would affect resistance of insects against insecticides is largely unknown, especially in agricultural pests. Here, we first reported that pre-exposure to two widely used herbicides butachlor and haloxyfop-methyl for 48 h can prime the resistance of a generalist agricultural pest Helicoverpa armigera Hübner against insecticide methomyl and fungal toxin aflatoxin B1. In addition, there were no significant differences between control and herbicides-treated caterpillars on weight gain, pupal weight, and pupation rates, suggesting that exposure to herbicides induces resistance of H. armigera accompanied with no fitness cost. Moreover, by determining detoxifying enzyme activities and toxicity bioassay with additional inhibitor of cytochrome P450 piperonyl butoxide (PBO), we showed that exposure to herbicides might prime P450-mediated detoxification of H. armigera against insecticide. Based on these results, we propose that exposure to herbicides prime resistance of H. armigera against insecticide and fungal toxin by eliciting a clear elevation of predominantly P450 monooxygenase activities in the midgut and fat body.
Muscle Involvement in a Large Cohort of Pediatric Patients with Genetic Diagnosis of Mitochondrial Disease
Authors: Jou Cristina , Ortigoza-Escobar Juan D. , O’Callaghan Maria M. , Nascimento Andres , Darling Alejandra , Pias-Peleteiro Leticia , Perez-Dueñas Belén , Pineda Mercedes , Codina Anna , Arjona César , Armstrong Judith , Palau Francesc , Ribes Antonia , Gort Laura , Tort Frederic , Navas Placido , Ruiz-Pesini Eduardo , Emperador Sonia , Lopez-Gallardo Ester , Bayona-Bafaluy Pilar , Montero Raquel , Jimenez-Mallebrera Cecilia , Garcia-Cazorla Angels , Montoya Julio , Yubero Delia , Artuch Rafael
Published: January 2019

Mitochondrial diseases (MD) are a group of genetic and acquired disorders which present significant diagnostic challenges. Here we report the disease characteristics of a large cohort of pediatric MD patients (n = 95) with a definitive genetic diagnosis, giving special emphasis on clinical muscle involvement, biochemical and histopathological features. Of the whole cohort, 51 patients harbored mutations in nuclear DNA (nDNA) genes and 44 patients had mutations in mitochondrial DNA (mtDNA) genes. The nDNA patients were more likely to have a reduction in muscle fiber succinate dehydrogenase (SDH) stains and in SDH-positive blood vessels, while a higher frequency of mtDNA patients had ragged red (RRF) and blue fibers. The presence of positive histopathological features was associated with ophthalmoplegia, myopathic facies, weakness and exercise intolerance. In 17 patients younger than two years of age, RRF and blue fibers were observed only in one case, six cases presented cytochrome c oxidase (COX) reduction/COX-fibers, SDH reduction was observed in five and all except one presented SDH-positive blood vessels. In conclusion, muscle involvement was a frequent finding in our series of MD patients, especially in those harboring mutations in mtDNA genes.
Type 2 Diabetes Is Associated with a Different Pattern of Serum Polyamines: A Case–Control Study from the PREDIMED-Plus Trial
Authors: Fernandez-Garcia Jose C. , Delpino-Rius Antoni , Samarra Iris , Castellano-Castillo Daniel , Muñoz-Garach Araceli , Bernal-Lopez Maria R. , Queipo-Ortuño Maria I. , Cardona Fernando , Ramos-Molina Bruno , Tinahones Francisco J.
Published: January 2019

Objective: Polyamines are naturally occurring cationic molecules present in all living cells. Dysregulation of circulating polyamines has been reported in several conditions, but little is known about the levels of serum polyamines in chronic metabolic disorders such as type 2 diabetes (T2D). Therefore, the aim of this study was to evaluate the polyamine-related metabolome in a cohort of metabolic syndrome individuals with and without T2D. Design and methods: This was a nested case–control study within the PREDIMED-Plus trial that included 44 patients with T2D and 70 patients without T2D. We measured serum levels of arginine, ornithine, polyamines, and acetyl polyamines with an ultra-high performance liquid chromatography tandem mass spectrometry platform. Results: Our results showed that serum putrescine, directly generated from ornithine by the catalytic action of the biosynthetic enzyme ornithine decarboxylase, was significantly elevated in patients with T2D compared to those without T2D, and that it significantly correlated with the levels of glycosylated hemoglobin (HbA1c). Correlation analysis revealed a significantly positive association between fasting insulin levels and spermine. Multiple logistic regression analysis (adjusted for age, gender and body weight index) revealed that serum putrescine and spermine levels were associated with a higher risk of T2D. Conclusions: Our study suggests that polyamine metabolism is dysregulated in T2D, and that serum levels of putrescine and spermine are associated with glycemic control and circulating insulin levels, respectively.
Waist Circumference and All-Cause Mortality Independent of Body Mass Index in Korean Population from the National Health Insurance Health Checkup 2009–2015
Authors: Kim Yang-Hyun , Kim Seon Mee , Han Kyung-Do , Jung Jin-Hyung , Lee Seong-Su , Oh Sang Woo , Park Hye Soon , Rhee Eun-Jung , Lee Won-Young , Yoo Soon Jib
Published: January 2019

Background: Waist circumference (WC) is an index of abdominal obesity and associated with co-morbidities and mortality. Higher WC is positively associated with increased mortality; therefore, we examined the relationship between WC and mortality in Korean populations with the interaction of body mass index (BMI) and WC for mortality. Methods: A total of 23,263,878 subjects (men = 11,813,850 and women = 11,450,028) who were older than 20 years and underwent the National Health Insurance Service health checkup were included. WC was divided into six categories by 5 cm increments and level 3 (85–90 cm in men and 80–85 cm in women) was referenced. Multivariable Cox proportional hazard models were used to obtain the hazard ratios (HRs) and 95% confidence intervals for all-cause mortality according to the six levels of WC. Results: WC in 5 cm increments showed a positively increased all-cause mortality after adjusting for all covariates including BMI. Men showed higher HRs for mortality than women as WC increased, and the HRs were higher in the lower WC levels, but lower in the higher WC levels among the subjects aged 65–85 years than subjects aged 40–65 years. Even in subjects with normal weight and overweight, increased WC (levels 4, 5, and 6) showed increased HRs for mortality (HRs = 1.156, 1.412, and 1.614 in normal BMI and 1.145, 1.401, and 1.909 in overweight, respectively). Conclusion: There was a linear association between WC and all-cause mortality across all BMI categories even in the subjects with normal or overweight BMI. Physicians should check WC routinely even in the subjects with normal weight or overweight.
Intake of Lutein and Zeaxanthin as a Possible Factor Influencing Gastrointestinal Symptoms in Caucasian Individuals with Ulcerative Colitis in Remission Phase
Authors: Głąbska Dominika , Guzek Dominika , Zakrzewska Paulina , Lech Gustaw
Published: January 2019

The vitamin A deficiencies are commonly observed in the case of ulcerative colitis individuals. The decreased antioxidant defence may influence the intestine, inducing higher susceptibility to oxidative damage of tissues and altering the symptoms and course of disease. Intestinal symptoms, ranging from diarrhea to constipation, occur more commonly in remission ulcerative colitis individuals than in general population. The aim of the study was to analyze the association between retinoid intake and gastrointestinal symptoms in Caucasian individuals in the remission phase of ulcerative colitis. Reitnoid (total vitamin A, retinol, β-carotene, α-carotene, β-cryptoxanthin, lycopene, as well as lutein and zeaxanthin) intakes were analyzed on the basis of three-day dietary records. Gastrointestinal symptoms (daily number of bowel movements, and the presence of painful tenesmus, flatulence, and constipation) were self-reported. The study was conducted in a group of 56 ulcerative colitis remission individuals, stratified by the gastrointestinal symptoms. One in every seven individuals reported recurring constipation. Higher intake of lutein and zeaxanthin (median 1386.2 µg, 289.0–13221.3 µg vs. median 639.0 µg, 432.7–1309.0 µg) may lower the incidence of constipation (p = 0.013). The intake of retinoids other than lutein and zeaxanthin was not associated with the incidence of constipation or other gastrointestinal symptoms.
The Impact of Cholecystectomy on the Gut Microbiota: A Case-Control Study
Authors: Yoon Won Jae , Kim Han-Na , Park Eunkyo , Ryu Seungho , Chang Yoosoo , Shin Hocheol , Kim Hyung-Lae , Yi Sun Young
Published: January 2019

Cholecystectomy alters the bile flow into the intestine and the enterohepatic circulation of the bile acids; this may affect the gut microbiota. We assessed the gut microbiota composition of patients who had undergone cholecystectomy and compared with those who had not. From a cohort of 1463 adult participants who underwent comprehensive health screening examinations, 27 subjects who had undergone cholecystectomy (cholecystectomy group) and 81 age- and sex-matched subjects who had not (control group) were selected. Clinical parameters were collected and compared. Microbial composition was determined by 16S rRNA gene sequencing of DNA extracted from fecal samples. We evaluated differences in the overall microbial composition and in the abundance of taxa. The two groups were comparable with respect to clinical characteristics and laboratory results. The actual number of taxa observed in a sample (observed features) was significantly lower in the cholecystectomy group than in the control group (p = 0.042). The beta diversity of Jaccard distance index was significantly different between the two groups (p = 0.027). Blautia obeum and Veillonella parvula were more abundant in the cholecystectomy group. The difference in the diversity of the gut microbiota between the cholecystectomy and control groups was subtle. However, B. obeum and V. parvula, which have azoreductase activity, were more abundant in the cholecystectomy group. The impact of such changes in the gut microbiota on health remains to be determined.
Bayesian Statistics of Wide-Band Radar Reflections for Oil Spill Detection on Rough Ocean Surface
Authors: Hammoud Bilal , Ndagijimana Fabien , Faour Ghaleb , Ayad Hussam , Jomaah Jalal
Published: January 2019

In this paper, we present a probabilistic approach which uses nadir-looking wide-band radar to detect oil spills on rough ocean surface. The proposed approach combines a single-layer scattering model with Bayesian statistics to evaluate the probability of detection of oil slicks, within a plausible range of thicknesses, on seawater. The difference between several derived detection algorithms is defined in terms of the number of frequencies used (within C-to-X-band ranges), as well as of the number of radar observations. Performance analysis of all three types of detectors (single-, dual- and tri-frequency) is done under different surface-roughness scenarios. Results show that the probability of detecting an oil slick with a given thickness is sensitive to the radar frequency. Multi-frequency detectors prove their ability to overcome the performance of the single- and dual-frequency detectors. Higher probability of detection is obtained when using multiple observations. The roughness of the ocean surface leads to a loss in the reflectivity values, and therefore decreases the performance of the detectors. A possible way to make use of the drone systems in the contingency planning is also presented.
Exploring Marine and Aeolian Controls on Coastal Foredune Growth Using a Coupled Numerical Model
Authors: Cohn Nicholas , Hoonhout Bas M. , Goldstein Evan B. , De Vries Sierd , Moore Laura J. , Durán Vinent Orencio , Ruggiero Peter
Published: January 2019

Coastal landscape change represents aggregated sediment transport gradients from spatially and temporally variable marine and aeolian forces. Numerous tools exist that independently simulate subaqueous and subaerial coastal profile change in response to these physical forces on a range of time scales. In this capacity, coastal foredunes have been treated primarily as wind-driven features. However, there are several marine controls on coastal foredune growth, such as sediment supply and moisture effects on aeolian processes. To improve understanding of interactions across the land-sea interface, here the development of the new Windsurf-coupled numerical modeling framework is presented. Windsurf couples standalone subaqueous and subaerial coastal change models to simulate the co-evolution of the coastal zone in response to both marine and aeolian processes. Windsurf is applied to a progradational, dissipative coastal system in Washington, USA, demonstrating the ability of the model framework to simulate sediment exchanges between the nearshore, beach, and dune for a one-year period. Windsurf simulations generally reproduce observed cycles of seasonal beach progradation and retreat, as well as dune growth, with reasonable skill. Exploratory model simulations are used to further explore the implications of environmental forcing variability on annual-scale coastal profile evolution. The findings of this work support the hypothesis that there are both direct and indirect oceanographic and meteorological controls on coastal foredune progradation, with this new modeling tool providing a new means of exploring complex morphodynamic feedback mechanisms.
Sporotrichosis In Immunocompromised Hosts
Authors: Queiroz-Telles Flavio , Buccheri Renata , Benard Gil
Published: January 2019

Sporotrichosis is a global implantation or subcutaneous mycosis caused by several members of the genus Sporothrix, a thermo-dimorphic fungus. This disease may also depict an endemic profile, especially in tropical to subtropical zones around the world. Interestingly, sporotrichosis is an anthropozoonotic disease that may be transmitted to humans by plants or by animals, especially cats. It may be associated with rather isolated or clustered cases but also with outbreaks in different periods and geographic regions. Usually, sporotrichosis affects immunocompetent hosts, presenting a chronic to subacute evolution course. Less frequently, sporotrichosis may be acquired by inhalation, leading to disseminated clinical forms. Both modes of infection may occur in immunocompromised patients, especially associated with human immunodeficiency virus (HIV) infection, but also diabetes mellitus, chronic alcoholism, steroids, anti-TNF treatment, hematologic cancer and transplanted patients. Similar to other endemic mycoses caused by dimorphic fungi, sporotrichosis in immunocompromised hosts may be associated with rather more severe clinical courses, larger fungal burden and longer periods of systemic antifungal therapy. A prolonged outbreak of cat-transmitted sporotrichosis is in progress in Brazil and potentially crossing the border to neighboring countries. This huge outbreak involves thousands of human and cats, including immunocompromised subjects affected by HIV and FIV (feline immunodeficiency virus), respectively. We reviewed the main epidemiologic, clinical, diagnostic and therapeutic aspects of sporotrichosis in immunocompromised hosts.
How to Develop IoT Cloud e-Health Systems Based on FIWARE: A Lesson Learnt
Authors: Celesti Antonio , Fazio Maria , Galán Márquez Fermín , Glikson Alex , Mauwa Hope , Bagula Antoine , Celesti Fabrizio , Villari Massimo
Published: January 2019

Nowadays, the penetration of sensors and actuators in different application fields is revolutionizing all aspects of our daily life. One of the major sectors that is taking advantage of such cutting-edge cheap smart devices is healthcare. In this context, Remote Patient Monitoring (RPM) at home represents a tempting opportunity for hospitals to reduce clinical costs and to improve the quality of life of both patients and their families. It allows patients to be monitored remotely by means networks of Internet of Things (IoT) medical devices equipped with sensors and actuators that collect healthcare data from patients and send them to a Cloud-based Hospital Information System (HIS) for processing. Up to now, many different proprietary software systems have been developed as stand-along expensive solutions, presenting interoperability, extensibility, and scalability issues. In recent years, the European Commission (EC) has promoted the wide adoption of FIWARE technology, launching 16 Industrial Accelerators focusing on different application fields. One of these, i.e., FICHe, is specialized in healthcare, providing the guidelines on how to develop eHealth systems. This paper focuses on how to compose new cutting-edge IoT and Cloud-based Cyber Physical Health Sytem (CPHS) services and applications interconnected with remote medical sensors and actuators using FIWARE technology in the context envisioned by FICHe. In particular, we discuss the design and development of an RPM system implemented through the collaboration between the Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) “Bonino Pulejo” (i.e., a clinical and research healthcare centre specialized in the treatment of neuro lesions), University of Messina, IBM Research, Telefónica, and the University of the Western Cape in South Africa. The description of our best practice provides a model and guidelines for the development of lightweight and low cost RPM services for rural and isolated areas, with the expectation of expanding healthcare to the developing world and in general allows us to outline how to deal with the real adoption of the FIWARE technology in an e-health project.
Learning Labels for Objects: Does Degree of Sensorimotor Experience Matter?
Authors: Wellsby Michele , Pexman Penny
Published: January 2019

Theories of embodied cognition propose that sensorimotor experience is essential to learning, representing, and accessing conceptual information. Embodied effects have been observed in early child development and adult cognitive processing, but there has been less research examining the role of embodiment in later childhood. We conducted two experiments to test whether degree of sensorimotor experience modulates children’s word learning. In Experiment 1, 5-year-old children learned labels for 10 unfamiliar objects in one of six learning conditions, which varied in how much sensorimotor experience and information about the objects children received. Children’s word learning was assessed with a recognition test. Results indicated that there was no effect of learning condition on recognition accuracy, as children performed equally well in all conditions. In Experiment 2, we modified the stimuli to emphasize the sensory features of the objects; 5-year-old children learned labels for these objects in one of two learning conditions. Once again, there was no effect of learning condition on children’s recognition accuracy performance. Overall, children’s word learning was not modulated by the extent to which they had sensorimotor experience with the labelled objects. As such, the results place some limits on the role of embodiment in language learning.
Electrochemical Detection for Uric Acid Based on β-Lactoglobulin-Functionalized Multiwall Carbon Nanotubes Synthesis with PtNPs Nanocomposite
Authors: Han Bingkai , Pan Meixin , Liu Xinran , Liu Jian , Cui Teng , Chen Qiang
Published: January 2019

In this work, a simple and highly selective electrochemical biosensor for determination of uric acid (UA) is synthesized by using β-lactoglobulin (BLG)-functionalized multiwall carbon nanotubes (MWCNTs) and a platinum nanoparticles (PtNPs) nanocomposite. Urate oxidase (UOx) can oxidize uric acid to hydrogen peroxide and allantoin, which provides a good opportunity for electrochemical detection for UA. Under the optimized conditions, the current changes by the UOx/Bull Serum Albumin (BSA)/BLG-MWCNTs-PtNPs/Glassy Carbon (GC) electrode with the electrochemical method was proportional to the concentration of UA. According to experiments, we obtained a linear response with a concentration range from 0.02 to 0.5 mM and achieved a high sensitivity of 31.131 μA mM−1 and a low detection limit (0.8 μΜ). Meanwhile, nanoparticles improved the performance of the biosensor and combined with BLG not only prevented the accumulation of composite nanomaterials, but also provided immobilization of uricase through electrostatic adsorption. This improves the stability and gives the constructed electrode sensing interface superior performance in UA detection.
Solid-State Transformations in Inner Coordination Sphere of [Co(NH3)6][Fe(C2O4)3]∙3H2O as a Route to Access Catalytically Active Co-Fe Materials
Authors: Domonov Denis P. , Pechenyuk Sophia I. , Semushina Yulia P. , Yusenko Kirill V.
Published: January 2019

Thermal decomposition of [Co(NH3)6][Fe(C2O4)3]∙3H2O in argon atmosphere, at a low heating rate (3°/min), and in large amounts of the initial complex (~0.1 mole), has been studied. It was possible to distinguish four decomposition steps upon heating: In the temperature range of 50–100 °C—the loss of crystal water; 100–190 °C—stability region of dehydrated complex; 230–270 °C—the range of stability of intermediate phase with the formula CoFe(NH3)2(C2O4)2; 270–350 °C—thermal decomposition of the intermediate with the formation of metallic products and further air oxidation with the formation of Co1.5Fe1.5O4. Catalytic properties of thermolysis products were tested in the decomposition reaction of H2O2 (inactive), oxidation of acetone (average activity), and decomposition of ammonium perchlorate (highly active).
Microstructures and Isothermal Oxidation of the Alumina Scale Forming Nb1.7Si2.4Ti2.4Al3Hf0.5 and Nb1.3Si2.4Ti2.4Al3.5Hf0.4 Alloys
Authors: Ghadyani Mohammad , Utton Claire , Tsakiropoulos Panos
Published: January 2019

Nb–silicide based alloy will require some kind of coating system. Alumina forming alloys that are chemically compatible with the Nb–silicide based alloy substrate could be components of such systems. The intermetallic alloys Nb1.7Si2.4Ti2.4Al3Hf0.5 and Nb1.3Si2.4Ti2.4Al3.5Hf0.4 were studied in the cast, heat treated and isothermally oxidised conditions at 800 and 1200 °C to find out if they are alumina scale formers. The alloys were designed using the alloy design methodology NICE and were required (i) not to have stable solid solution phase in their microstructures; (ii) not to pest and (iii) to form alumina scale. Their microstructures consisted of silicides and aluminides. Both alloys satisfied (i) and (ii) and formed thin scales at 800 °C. At 1200 °C the former alloy suffered from internal oxidation and formed alumina intermixed with Ti rich oxide beneath a thick “layered” scale of mixed oxides that contained Ti and/or Al and/or Si. There was no internal oxidation in the latter alloy that formed a thin continuous well adhering α-Al2O3 scale that was able to repair itself during oxidation at 1200 °C. In both alloys there was severe macrosegregation of Si, which in Nb1.3Si2.4Ti2.4Al3.5Hf0.4 was almost double that in Nb1.7Si2.4Ti2.4Al3Hf0.5. The severe macrosegregation of Si contributed to the formation of a “layered” structure in the former alloy that was retained at 800 and 1200 °C. Both alloys met the “standard definition” of High Entropy Alloys (HEAs). Compared with the range of values of the parameters valence band (VEC), δ and Δχ of bcc solid solution plus intermetallic(s) HEAs, only the Δχ of the alloy Nb1.7Si2.4Ti2.4Al3Hf0.5 was within the range and the parameters VEC and δ of both alloys respectively were outside and within the corresponding ranges. The alloy Nb1.3Si2.4Ti2.4Al3.5Hf0.4 exhibited strong correlations between the parameters Δχ, δ and VEC, and the range of values of each parameter was wider compared with the alloy Nb1.7Si2.4Ti2.4Al3Hf0.5. There was a strong correlation only between the parameters Δχ and δ of the latter alloy that was similar to that of the former alloy.
Nepafenac-Loaded Cyclodextrin/Polymer Nanoaggregates: A New Approach to Eye Drop Formulation
Authors: Lorenzo-Veiga Blanca , Sigurdsson Hakon Hrafn , Loftsson Thorsteinn
Published: January 2019

The topical administration route is commonly used for targeting therapeutics to the eye; however, improving the bioavailability of drugs applied directly to the eye remains a challenge. Different strategies have been studied to address this challenge. One of them is the use of aggregates that are formed easily by self-assembly of cyclodextrin (CD)/drug complexes in aqueous solution. The aim of this study was to design a new eye drop formulation based on aggregates formed between CD/drug complexes. For this purpose, the physicochemical properties of the aggregates associated with six CDs and selected water-soluble polymers were analysed. Complex formation was studied using differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR) and 1H nuclear magnetic resonance spectroscopy (1H-NMR). Results showed that HPβCD performed best in terms of solubilization, while γCD performed best in terms of enhancing nanoaggregate formation. Formation of inclusion complexes was confirmed by DSC, FT-IR and 1H-NMR studies. A mixture of 15% (w/v) γCD and 8% (w/v) HPβCD was selected for formulation studies. It was concluded that formulations with aggregate sizes less than 1 µm and viscosity around 10–19 centipoises can be easily prepared using a mixture of CDs. Formulations containing polymeric drug/CD nanoaggregates represent an interesting strategy for enhanced topical delivery of nepafenac.
Nanocellulose-Block Copolymer Films for the Removal of Emerging Organic Contaminants from Aqueous Solutions
Authors: Herrera-Morales Jairo , Turley Taylor A. , Betancourt-Ponce Miguel , Nicolau Eduardo
Published: January 2019

The prevalence of emerging organic contaminants (EOCs) in ground and surface water has sparked the search for more effective methods to remove EOCs from the environment. In pursuit of a solution for this environmental concern, herein we present the development of reusable films based on cellulose nanofibers (CNFs) and the block copolymer, poly(4-vinylpyridine-b-ethylene oxide) (P4VP-PEO) to adsorb sulfamethoxazole (SMX) as an EOC model compound. We hypothesize that the adsorption of SMX was achieved mainly by π-π interactions between the pyridine functionalities of the block copolymer and the electron deficient phenyl group of the SMX. Preceding preparation of the films, CNFs were modified with the alkoxysilane trimethoxy(2-phenylethyl)silane (TMPES) to increase their stability in aqueous solution. After the addition of P4VP-PEO, the process was completed by filtration followed by oven-drying. XPS and FTIR were employed to confirm the addition of TMPES and P4VP-PEO, respectively. Adsorption batch experiments were performed in aqueous solutions of SMX at a neutral pH, obtaining adsorptions of up to 0.014 mmol/g in a moderate time of 60 min. For the reusability tests, films were immersed in ethanol 95 wt.% to elude the adsorbed SMX, rinsed with deionized (DI) water, and dried at room temperature to be reused in a new adsorption cycle. We found that this new composite material could be reused several times with negligible loss of adsorption capacity. The films presented have been shown to be of substantial importance for water remediation as they find direct application in the adsorption of electron deficient aromatic compounds and are reusable.
Effect of Scanning and Support Strategies on Relative Density of SLM-ed H13 Steel in Relation to Specimen Size
Authors: Kurzynowski Tomasz , Stopyra Wojciech , Gruber Konrad , Ziółkowski Grzegorz , Kuźnicka Bogumiła , Chlebus Edward
Published: January 2019

Standard experimental research works are aimed at optimization of Selective Laser Melting (SLM) parameters in order to produce material with relative density over 99% and possibly the highest scanning speed. Typically, cuboidal specimens with arbitrarily selected dimensions are built. An optimum set of parameters, determined on such specimens, is used for building parts with variable cross-section areas. However, it gives no guarantee that the density of variable-section parts produced with so selected parameters will be as high as that of the specimens measured during the parameters optimization process. The goal of this work was to improve the process of SLM parameter selection according to the criterion of maximum relative density, based on the example of AISI H13 tool steel (1.2344). A selection method of scanning strategy ensuring relative density of parts over 99%, irrespective of their dimensions, was determined. The specimens were produced using several variants of support structures. It was found that proper selection of the support strategy prevents development of columnar pores.
Acknowledgement to Reviewers of Machines in 2018
Authors:   Machines Editorial Office
Published: January 2019

Rigorous peer-review is the corner-stone of high-quality academic publishing [...]
Modelling the Effect of Zero-Field Splitting on the 1H, 13C and 29Si Chemical Shifts of Lanthanide and Actinide Compounds
Authors: Lloyd Austin W. , Moylan Helen M. , McDouall Joseph J. W.
Published: January 2019

The prediction of paramagnetic NMR (pNMR) chemical shifts in molecules containing heavy atoms presents a significant challenge to computational quantum chemistry. The importance of meeting this challenge lies in the central role that NMR plays in the structural characterisation of chemical systems. Hence there is a need for reliable assignment and prediction of chemical shifts. In a previous study [Trends in Physical Chemistry, 17, 25–57, (2017)] we looked at the computation of pNMR chemical shifts in lanthanide and actinide complexes using a spin Hamiltonian approach. In that study we were principally concerned with molecules with S = 1/2 ground states. In the present work we extend that study by looking at the effect of zero field splitting (ZFS) for six complexes with S = 3/2 ground states. It is shown that the inclusion of ZFS can produce substantial shifts in the predicted chemical shifts. The computations presented are typically sufficient to enable assignment of experimental spectra. However for one case, in which the peaks are closely clustered, the inclusion of ZFS re-orders the chemical shifts making assignment quite difficult. We also observe, and echo, the previously reported importance of including the paramagnetic spin-orbit hyperfine interaction for 13 C and 29 Si atoms, when these are directly bound to a heavy element and thus subject to heavy-atom-light-atom effects. The necessary computations are very demanding, and more work is needed to find theoretical and computational approaches that simplify the evaluation of this term. We discuss the computation of each term required in the spin Hamiltonian. The systems we study in this work are restricted to a single heavy atom ion (one Nd(III) and five U(III) complexes), but typify some of the computational complexity encountered in lanthanide and actinide containing molecules.
A Dilation Invariance Method and the Stability of Inhomogeneous Wave Equations
Authors: Choi Ginkyu , Jung Soon-Mo
Published: January 2019

We apply the method of a kind of dilation invariance to prove the generalized Hyers-Ulam stability of the (inhomogeneous) wave equation with a source, u t t ( x , t ) − c 2 ▵ u ( x , t ) = f ( x , t ) , for a class of real-valued functions with continuous second partial derivatives in each of spatial and the time variables.
Some Root Level Modifications in Interval Valued Fuzzy Graphs and Their Generalizations Including Neutrosophic Graphs
Authors: Jan Naeem , Ullah Kifayat , Mahmood Tahir , Garg Harish , Davvaz Bijan , Saeid Arsham Borumand , Broumi Said
Published: January 2019

Fuzzy graphs (FGs) and their generalizations have played an essential role in dealing with real-life problems involving uncertainties. The goal of this article is to show some serious flaws in the existing definitions of several root-level generalized FG structures with the help of some counterexamples. To achieve this, first, we aim to improve the existing definition for interval-valued FG, interval-valued intuitionistic FG and their complements, as these existing definitions are not well-defined; i.e., one can obtain some senseless intervals using the existing definitions. The limitations of the existing definitions and the validity of the new definitions are supported with some examples. It is also observed that the notion of a single-valued neutrosophic graph (SVNG) is not well-defined either. The consequences of the existing definition of SVNG are discussed with the help of examples. A new definition of SVNG is developed, and its improvement is demonstrated with some examples. The definition of an interval-valued neutrosophic graph is also modified due to the shortcomings in the current definition, and the validity of the new definition is proved. An application of proposed work is illustrated through a decision-making problem under the framework of SVNG, and its performance is compared with existing work.
Evaluation of Turmeric Nanoparticles as Anti-Gout Agent: Modernization of a Traditional Drug
Authors: Mustafa Kiyani Mubin Mustafa , Sohail Muhammad Farhan , Shahnaz Gul , Rehman Hamza , Akhtar Muhammad Furqan , Nawaz Irum , Mahmood Tariq , Manzoor Mobina , Imran Bokhari Syed Ali
Published: January 2019

Background and objectives: Turmeric has assisted in the control of inflammation and pain for decades and has been used in combination with other nutraceuticals to treat acute and chronic osteoarthritis pain. Recently, the effect of turmeric, turmeric extract, or curcuminoids on musculoskeletal pain, either by themselves or in conjunction with other substances, has been reported. The aim of this study was to develop and characterize turmeric nanoparticles (T-NPs) for various parameters, both in vitro and in vivo. Materials and Methods: The T-NPs were successfully synthesized and characterized using particle size analysis, solubility improvement, SEM, EDX, X-ray diffraction, and in vivo antigout activity in mice model. Results: The T-NPs were of about 46 nm in size with a positive zeta potential +29.55 ± 3.44 and low polydispersity index (PDI) (0.264). Furthermore, the diseased mice, with induced gout via monosodium urate crystals, were treated with 5, 10, and 20 ppm T-NPs, administered orally, and the anti-gout potential was observed through measurement of joint diameter and changes in biochemical parameters, including lipid profile, renal function test, and liver function tests which significantly reduced the levels of these biochemical parameters. Conclusions: Uric acid levels were significantly reduced after the treatment with T-NPs. indicating that T-NPs show superior potential against gout management. Thus, T-NPs can be developed as an efficient antigout agent with minimum toxicities.