bluuebunny/crossref_metadata_2025 huggingface dataset:
Migration, culture contact and the complexity of coexistence
Authors: Formenti Laura , Luraschi Silvia
Published: October 2020

This paper covers a part of a larger qualitative and participatory study on the integration of asylum seekers and refugees hosted in the Province of Lecco, Northern Italy. Their embodied experience as newcomers and the daily relationships with space, with others, and with the public discourse is the main focus of the paper. The notion of coexistence, its struggles, constraints and possibilities, is addressed following Bateson’s work on culture contact and a systemic theoretical framework. The discourse of integration is deconstructed and challenged, re-imagining the outcome of culture contact as the unpredictable effect of complex, relational, and entangled processes of interaction. Newcomers’ movements in the physical and symbolic space are studied with aesthetic and generative methods, inspired by the spatial and sensory turns in social sciences. The methodology is multiple and layered: interviews, focus groups, narrative aesthetic workshops and sensobiographic walks are used to chronicle and interpret narratives of coexistence, at a micro, meso, and macro level.
Phytoextraction with Maize of Soil Contaminated with Copper after Application of Mineral and Organic Amendments
Authors: Wyszkowski Mirosław , Brodowska Marzena S.
Published: October 2020

This study aimed to determine the possibility to increase the the effect of different amendments (compost, bentonite and zeolite) on the shoot yield and the concentration of trace elements in shoots of maize (Zea mays L.) on soil contaminated with Cu. The yield of shoots and concentration of the trace elements in shoots of maize depended on Cu dose and amendment incorporation into the sandy soil. Cu-spiked soil caused an increase the yield of shoots (only to 100 mg Cu/kg of soil), in the concentration of Cu, Co, Mn, Ni and Fe in shoots of maize and, to a smaller degree, in the concentration of Zn and bioconcentration factor (BCF) of all elements except copper, compared to the control soil without Cu. Under the influence of 150 and 200 mg Cu per kg of soil, a decrease in yield of shoots of maize was observed. Compost, bentonite and zeolite increased the yield of shoots and reduced the concentration of Cu, Co, Mn, Fe and Zn in shoots of maize. Bentonite had a more positive effect than compost and zeolite on the yield of shoots and the concentration of Co, Mn and Zn in shoots of maize. The effect of these amendments on the Cu and Fe concentration in shoots of maize was reverse. A reverse effect of these amendments (especially bentonite and zeolite) on the Ni concentration in plants was observed. The amendments applied to soil, especially compost, increased the BCF of Ni and, to a small degree, BCF of Cu in shoots of maize, compared to the control series. Compost, zeolite and especially bentonite are very good amendments in the restoration of maize growth in polluted areas.
Warming Reduces Net Carbon Gain and Productivity in Medicago sativa L. and Festuca arundinacea
Authors: Jacob Vinod , Zhang Haiyang , Churchill Amber C. , Yang Jinyan , Choat Brendan , Medlyn Belinda E. , Power Sally A. , Tissue David T.
Published: October 2020

High temperature stress imposes constraints on the productivity of agricultural systems, such as pastures, and predicted increases in global temperatures are set to exacerbate these limitations. Here, we sought to understand the impact of warmer growth temperature on gas exchange and net primary productivity for two widely cultivated pasture species. We grew a C3 legume, Medicago sativa (lucerne), and a C3 grass, Festuca arundinacea Schreb. (tall fescue), in a climate-controlled facility exposed to two temperature treatments (ambient: 26 °C, aT; elevated: 30 °C, eT). Soil water was maintained at non-limiting conditions in both temperature treatments to control for the confounding effects of warming on soil moisture. We found that warming reduced photosynthetic capacity and increased leaf dark respiration (Rdark) in lucerne, while tall fescue showed little physiological change at the leaf level, but increased ecosystem respiration (Reco). Growth temperature had no significant impact on the thermal optimum of photosynthesis (Topt) or water use efficiency in either species. Both species exhibited significant reductions in productivity with warming; lucerne had greater reductions in shoot biomass, while tall fescue had greater reductions in root biomass. Our results highlight the potential for significant declines in pasture productivity associated with even modest increases in average temperature and highlights the need for suitable management strategies and implementation of more heat-resistant cultivars. Improvements in photosynthetic performance for greater heat tolerance in lucerne, and traits associated with biomass allocation and root performance at higher temperatures in tall fescue, should be the focus for improving high temperature resistance in these plant species.
Genetic Signatures of Selection for Cashmere Traits in Chinese Goats
Authors: Jin Meilin , Lu Jian , Fei Xiaojuan , Lu Zengkui , Quan Kai , Liu Yongbin , Chu Mingxing , Di Ran , Wang Huihua , Wei Caihong
Published: October 2020

Inner Mongolia and Liaoning cashmere goats in China are well-known for their cashmere quality and yield. Thus, they are great models for identifying genomic regions associated with cashmere traits. Herein, 53 Inner Mongolia cashmere goats, Liaoning cashmere goats and Huanghuai goats were genotyped, and 53,347 single-nucleotide polymorphisms (SNPs) were produced using the Illumina Caprine 50K SNP chip. Additionally, we identified some positively selected SNPs by analyzing Fst and XP-EHH. The top 5% of SNPs had selection signatures. After gene annotation, 222 and 173 candidate genes were identified in Inner Mongolia and Liaoning cashmere goats, respectively. Several genes were related to hair follicle development, such as TRPS1, WDR74, LRRC14, SPTLC3, IGF1R, PADI2, FOXP1, WNT10A and CSN3. Gene enrichment analysis of these cashmere trait-associated genes related 67 enriched signaling pathways that mainly participate in hair follicle development and stem cell pluripotency regulation. Furthermore, we identified 20 overlapping genes that were selected in both cashmere goat breeds. Among these overlapping genes, WNT10A and CSN3, which are associated with hair follicle development, are potentially involved in cashmere production. These findings may improve molecular breeding of cashmere goats in the future.
A Retrospective Study after 10 Years (2010–2019) of Meat Inspection Activity in a Domestic Swine Abattoir in Tuscany: The Slaughterhouse as an Epidemiological Observatory
Authors: Guardone Lisa , Vitali Alessio , Fratini Filippo , Pardini Stefano , Cenci Goga Beniamino Terzo , Nucera Daniele , Armani Andrea
Published: October 2020

The activities performed by the Official Veterinarian at the slaughterhouse represent a useful source of data for the control of issues affecting human and animal health and welfare. This study analyzed the data deriving from ten years (2010–2019) of meat inspection in a pig slaughterhouse in Tuscany (Italy) to investigate the transport mortality rate and the frequencies and main causes of whole carcass and partial condemnations. In total, 1,246,309 pigs were slaughtered from 8 different regions of Central and Northern Italy. Overall, 1153 pigs died during transport (mortality rate 0.09%). Whole carcass condemnation affected 372 carcasses (0.03%), mainly due to erysipelas, generalized jaundice, lipomatous pseudohypertrophy, generalized abscesses, acute or generalized enteritis, and peritonitis. As regards partial condemnations, the liver was the most frequently condemned (~30% of the pigs), followed by lungs (17.3%), heart (6.9%), and kidney (0.9%). The main causes were “milk spot liver” and perihepatitis for the liver; pneumonia and pleurisy for the lungs; pericarditis and polyserositis for the heart; and polycystic kidney and nephritis for kidneys. The results of this study describe a non-worrying situation as regards the investigated aspects and confirm the slaughterhouse as a valid epidemiological observatory for monitoring the trend of the main diseases over time, the results of the efforts for their control, and the compliance with animal welfare standards.
Carcass Characteristics and Primal Pork Cuts of Gilts, Boars, Immunocastrates and Barrows Using AutoFOM III Data of a Commercial Abattoir
Authors: Kress Kevin , Hartung Jens , Jasny Johannes , Stefanski Volker , Weiler Ulrike
Published: October 2020

The surgical castration of male piglets as a routine procedure in modern pig production is facing increasing societal criticism. Pork production with boars and immunocastrates are available alternatives, but both have low market shares as it is so far uncertain how the carcass characteristics and primal pork cuts of boars and immunocastrates will be estimated in comparison to barrows and gilts. This article therefore evaluates the impact of sex group (gilts, boars, immunocastrates and barrows) on carcass characteristics and primal pork cuts using AutoFOM III data from a commercial abattoir. In our study, weekly slaughter data from a total of n = 36,994 pigs between 2018 and 2019 were analyzed. The results show that gilts had the highest amount of pork per carcass of all sex groups, whereas non-significant differences between boars, immunocastrates and barrows could be observed. Boars had the highest lean meat content, followed by gilts, immunocastrates and finally barrows with the lowest lean meat content. These results suggest that both immunocastration and pork production with boars are sustainable techniques that can replace pork production with barrows without affecting carcass quality.
Comparative Analysis of Intestinal Helminth Infections in Colic and Non-Colic Control Equine Patients
Authors: Gehlen Heidrun , Wulke Nadine , Ertelt Antonia , Nielsen Martin K. , Morelli Simone , Traversa Donato , Merle Roswitha , Wilson Douglas , Samson-Himmelstjerna Georg von
Published: October 2020

All around the world, intestinal helminths constitute one of the most prevalent life-long occurring infections and re-infections affecting all horse age groups. A range of parasite species among strongyles, ascarids, and tapeworms is known to have the potential to cause colic in horses. However, there is a lack of current scientific evidence on the actual relevance of helminth infection levels in the context of colic in horses kept during prevailing epidemiological conditions. Thus, a prospective case-control study on the occurrence of intestinal helminths in a total of 620 mainly adult equine clinic patients was conducted to investigate the association between colic and helminth infection. For each horse, a range of copromicroscopic, serological, and clinical data was obtained, in addition to a questionnaire on relevant anamnestic data, including previous anthelmintic treatment and husbandry. Using a FLOTAC-based copromicroscopic diagnosis, the highest infection rates were seen for strongyles (41.8%), followed by Anoplocephala perfoliata and Parascaris spp. (both 0.8%), with no significant difference between the two study groups. Employing a real-time PCR a 1.1% S. vulgaris DNA prevalence was found. Considerably higher seroprevalences were observed using S. vulgaris and A. perfoliata ELISAs, with 32.3% and 10.7%, respectively. It was noteworthy that no association concerning either serologic status was encountered with colic status. The shedding of strongyle eggs was associated with a 1.8-times increased risk of S. vulgaris seropositivity. Recent anthelmintic treatment was associated with the onset of colic, as animals who had received an anthelmintic during the previous week had a 2.4-times higher risk of signs of colic compared to those who had been treated at least eight weeks prior. Another noteworthy observation was that ponies were significantly less often affected by colic than warmbloods. The high S. vulgaris and considerable A. perfoliata seroprevalences encountered in this investigation should prompt veterinarians, farm managers, and horse owners to maintain consequent and effective worm control measures.
Traumatic Stress of Frontline Workers in Culling Livestock Animals in South Korea
Authors: Park Hyomin , Chun Myung Sun , Joo Yunjeong
Published: October 2020

The last decade brought several devastating outbreaks of foot and mouth disease and avian influenza in South Korea, which had been handled through preventive culling, despite the controversy surrounding its efficiency and ethical considerations. Notably, the lack of regulations on culling processes has exposed the workers to extremely harsh working conditions. This study investigates the effect of culling jobs on the mental health of the frontline workers, based on 200 samples collected through a web-based survey conducted on participants with experience of culling tasks. Culling was found to have a powerful negative effect on the workers’ mental health, including high depression rates. Of those surveyed, 83.7% answered that the working conditions were intense, and 74.5% showed scores above the cutoff point for post-traumatic stress disorder (PTSD). A regression analysis revealed that individual’s attitudes toward animals mediated the effect of culling experience on PTSD symptoms. However, mental health care for the workers has been insufficient: 70.2% of the respondents were willing to get mental treatment to deal with the distress they underwent from culling. We conclude that engagement in culling has a detrimental effect on the workers’ mental health, and that they should be provided with systematic mental health care.
Safety Evaluation of α-Lipoic Acid Supplementation: A Systematic Review and Meta-Analysis of Randomized Placebo-Controlled Clinical Studies
Authors: Fogacci Federica , Rizzo Manfredi , Krogager Christoffer , Kennedy Cormac , Georges Coralie M.G. , Knežević Tamara , Liberopoulos Evangelos , Vallée Alexandre , Pérez-Martínez Pablo , Wenstedt Eliane F.E. , Šatrauskienė Agnė , Vrablík Michal , Cicero Arrigo F.G.
Published: October 2020

Alpha-lipoic acid (ALA) is a natural short-chain fatty acid that has attracted great attention in recent years as an antioxidant molecule. However, some concerns have been recently raised regarding its safety profile. To address the issue, we aimed to assess ALA safety profile through a systematic review of the literature and a meta-analysis of the available randomized placebo-controlled clinical studies. The literature search included EMBASE, PubMed Medline, SCOPUS, Google Scholar, and ISI Web of Science by Clarivate databases up to 15th August 2020. Data were pooled from 71 clinical studies, comprising 155 treatment arms, which included 4749 subjects with 2558 subjects treated with ALA and 2294 assigned to placebo. A meta-analysis of extracted data suggested that supplementation with ALA was not associated with an increased risk of any treatment-emergent adverse event (all p > 0.05). ALA supplementation was safe, even in subsets of studies categorized according to smoking habit, cardiovascular disease, presence of diabetes, pregnancy status, neurological disorders, rheumatic affections, severe renal impairment, and status of children/adolescents at baseline.
Effect of Particle Orientation and Porosity on Thermal Conductivity of Petroleum Pitch Polymer-Based Carbon Molded Body
Authors: Cho Jong Hoon , Im Ji Sun , Bai Byong Chol
Published: October 2020

The present study was conducted to investigate changes in the thermal conductivity of petroleum pitch-based carbon molded bodies prepared by anisotropic (uniaxial) molding under different molding pressures. The carbon molded bodies were prepared using needle coke and petroleum-based binder pitch polymers (softening point: 150 ℃). Green blocks prepared under high molding pressure showed a higher particle orientation value up to 16.4 μm. Graphite blocks, prepared by graphitizing the green blocks at 2800 ℃ showed a similar trend. The pores in the carbon molded body were filled with low boiling point substances, generated by the thermal treatment of the binder pitch polymer or air that could not be discharged during the molding procedure. Therefore, when phonons encountered a pore, phonon scattering, rather than phonon transport, occurred, and thus the heat transport from the hot zone to a cold zone became slow. As a result, although the particle orientation was a little higher in the B_10-G sample than in the B_20-G sample (in the error range), the thermal conductivity was higher in the B_20-G sample, which may be because the B_10-G sample had a higher porosity than the B_20-G sample.
Predicting Intestinal and Hepatic First-Pass Metabolism of Orally Administered Testosterone Undecanoate
Authors: Zgair Atheer , Dawood Yousaf , Ibrahem Suhaib M. , Back Hyun-moon , Kagan Leonid , Gershkovich Pavel , Lee Jong Bong
Published: October 2020

The bioavailability of orally administered drugs could be impacted by intestinal and hepatic first-pass metabolism. Testosterone undecanoate (TU), an orally administered ester prodrug of testosterone, is significantly subjected to first-pass metabolism. However, the individual contribution of intestinal and hepatic first-pass metabolism is not well determined. Therefore, the aim of the current study was to predict the metabolic contribution of each site. The hydrolysis–time profiles of TU incubation in human liver microsomes and Caco-2 cell homogenate were used to predict hepatic and intestinal first-pass metabolism, respectively. The in vitro half-life (t1/2 inv) for the hydrolysis of TU in microsomal mixtures was 28.31 ± 3.51 min. By applying the “well-stirred” model, the fraction of TU that could escape hepatic first-pass metabolism (FH) was predicted as 0.915 ± 0.009. The incubation of TU in Caco-2 cell homogenate yielded t1/2 inv of 109.28 ± 21.42 min, which was applied in a “Q gut” model to estimate the fraction of TU that would escape intestinal first-pass metabolism (FG) as 0.114 ± 0.02. Accordingly, only 11% of the absorbed fraction of TU could escape intestinal metabolism, while 91% can pass through hepatic metabolism. Hence, compared to the liver, the intestinal wall is the main site where TU is significantly metabolised during first-pass effect.
Automatic Classification of Text Complexity
Authors: Santucci Valentino , Santarelli Filippo , Forti Luciana , Spina Stefania
Published: October 2020

This work introduces an automatic classification system for measuring the complexity level of a given Italian text under a linguistic point-of-view. The task of measuring the complexity of a text is cast to a supervised classification problem by exploiting a dataset of texts purposely produced by linguistic experts for second language teaching and assessment purposes. The commonly adopted Common European Framework of Reference for Languages (CEFR) levels were used as target classification classes, texts were elaborated by considering a large set of numeric linguistic features, and an experimental comparison among ten widely used machine learning models was conducted. The results show that the proposed approach is able to obtain a good prediction accuracy, while a further analysis was conducted in order to identify the categories of features that influenced the predictions.
Strength Evaluation and Modification of a 3D Printed Anterior Ankle Foot Orthoses
Authors: Lin Yi-Chen , Huang Li-Ying , Chen Chen-Sheng
Published: October 2020

Ankle foot orthosis (AFO) is widely used to prevent foot drop and improve walking ability for individuals with cerebral palsy and stroke. However, traditional anterior AFO (TAAFO) could only last within months because the bilateral neck of TAAFO was easy to break. Currently, a 3D-printing technique is used to develop assistive devices for rehabilitation. The study aimed to implement the finite element (FE) method to revise the 3D printed AAFO (3DP-AAFO) and evaluate its strength. A 3.2 mm-thickness for the TAAFOs and 3DP-AAFOs were fabricated, respectively. The stiffness of TAAFO and 3DP-AAFO were tested by a material machine and compared to the FE model. In the FE analysis, the thickness of AAFO model was increased at the neck to enhance its strength. A plantarflexion and dorsiflexion moment were respectively subjected to 3DP-AAFO models to undergo stress analysis. Under the mechanical test, the 3DP-AAFO (K = 1.09 Nm/degree) was 7.8 times stiffer than the traditional AAFO (K = 0.14 Nm/degree). The FE results showed that thickening the 3DP-AAFO on the neck up to 4.7 mm could moderate stress concentration and increase the stiffness of the 3DP-AAFO. Therefore, the study concluded that the 3DP-AAFO was stiffer than the traditional AAFO. Increasing the appropriate thickness around neck of 3DP-AAFO could avoid neck fracture as much as possible.
Biomechanical Comparison of Posterior Fixation Combinations with an Allograft Spacer between the Lateral Mass and Pedicle Screws
Authors: Lee Soo-Bin , Lee Hwan-Mo , Park Tae-Hyun , Lee Sung , Kwon Young-Woo , Moon Seong-Hwan , Lee Byung
Published: October 2020

Background: There are a few biomechanical studies that describe posterior fixation methods with pedicle screws (PS) and lateral mass screws (LMS); the combination of both screw types and their effect on an allograft spacer in a surgically treated cervical segment is unknown. Methods: Finite element model (FEM) analyses were used to investigate the effects of a hybrid technique using posterior PS and LMS. Stress distribution and subsidence risk from a combination of screws under hybrid motion control conditions, including flexion, extension, axial rotation, and lateral bending, were investigated to evaluate the biomechanical characteristics of different six-screw combinations. Findings: The load sharing on the allograft spacer in flexion mode was highest in the LMS model (74.6%) and lowest in the PS model (35.1%). The likelihood of subsidence of allograft spacer on C6 was highest in the screws from the distal LMS (type 5) model during flexion and extension (4.902 MPa, 30.1% and 2.189 MPa, 13.4%). In lateral bending, the left unilateral LMS (type 4) model screws on C5 (3.726 MPa, 22.9%) and C6 (2.994 MPa, 18.4%) yielded the greatest subsidence risks, because the lateral bending forces were supported by the LMS. In counterclockwise axial rotation, the left unilateral LMS (type 4) model screws on C5 (3.092 MPa, 19.0%) and C6 (3.076 MPa, 18.9%) demonstrated the highest subsidence risks. Conclusion: The asymmetrical ipsilateral use of LMS and posterior PS in lateral bending and axial rotation demonstrated the lowest stability and greatest subsidence risk. We recommend bilateral symmetrical insertion of LMS or posterior PS and posterior PS on distal vertebrae for increased stability and reduced risk of allograft spacer subsidence.
The Analytical Prediction of Thermal Distribution and Defect Generation of Inconel 718 by Selective Laser Melting
Authors: Yang Huadong , Li Zhen , Wang Siqi
Published: October 2020

In selective laser melting, the rapid change of the temperature field caused by the rapid movement of the laser causes the instability of the melt pool flow, resulting in a generation of defects, such as lack of fusion, keyholing and balling effect, which greatly affect the performance of parts. In order to fully understand the temperature distribution and defect generation process of selective laser melting (SLM), experimental research, numerical simulation and analytical methods are mainly applied. The analytical method is suitable for the determination of the optimal process parameters because it is simple and consumes fewer resources. In a simulation, the absorptivity of the material is usually regarded as a constant, but experimental studies have shown that absorptivity is related to temperature, laser power, scanning speed, layer thickness and other process parameters. Considering the dynamics of thermal physical properties of Inconel 718, an improved analytical method was proposed and successfully applied to thermal analysis and the prediction of melt pool size. By comparing with the results of finite element simulation, experiment and other analytical solutions, the ease of use and effectiveness of the method are verified. Based on the prediction of the melt pool and the criterion of internal defects, the combination of process parameters that produce internal defects is calculated, which will make it possible to quickly obtain ideal process parameters.
Measuring the Impact of Infectious Diseases on Tourists’ Willingness to Pay to Visit Island Destinations
Authors: León Carmelo J. , Lam-González Yen E. , Galindo Carmen García , Hernández Matías M. González
Published: October 2020

The occurrence of infectious diseases may change tourists’ perceptions of a destination’s image and value. This article proposes and empirically tests a choice model to measure the effect of the risk of infectious disease outbreaks caused by climate change on tourists’ willingness to pay for holidays to island destinations. With this aim, an online survey was administrated to 2538 European frequent travellers at their country of residence. Tourists were presented with a hypothetical situation whereby they had to choose among eleven well-known European island destinations for their next holiday. The choice cards included the probability of the occurrence of infectious disease events in the context of other potential risks caused by climate change (i.e., forest fires, floods, heat waves, etc.). The results show infectious disease is the risk that more negatively affects tourists’ willingness to pay to visit islands, followed by forest fires. The results have implications for tourism policy, highlighting the importance of prevention and response strategies, and the design of climate-oriented services, which may raise opportunities to work towards the enhancement of those health and environmental conditions of tourist destinations that ensure their sustainability in the longer term.
The Effect of the Covid-19 Lockdown on Air Quality in Three Italian Medium-Sized Cities
Authors: Donzelli Gabriele , Cioni Lorenzo , Cancellieri Mariagrazia , Llopis Morales Agustin , Morales Suárez-Varela Maria
Published: October 2020

Despite the societal and economic impacts of the COVID-19 pandemic, the lockdown measures put in place by the Italian government provided an unprecedented opportunity to increase our knowledge of the effect transportation and industry-related emissions have on the air quality in our cities. This study assessed the effect of reduced emissions during the lockdown period, due to COVID-19, on air quality in three Italian cities, Florence, Pisa, and Lucca. For this study, we compared the concentration of particulate matter PM10, PM2.5, NO2, and O3 measured during the lockdown period, with values obtained in the same period of 2019. Our results show no evidence of a direct relationship between the lockdown measures implemented and PM reduction in urban centers, except in areas with heavy traffic. Consistent with recently published studies, we did, however, observe a significant decrease in NO2 concentrations among all the air-monitoring stations for each city in this study. Finally, O3 levels remained unchanged during the lockdown period. Of note, there were slight variations in the meteorological conditions for the same periods of different years. Our results suggest a need for further studies on the impact of vehicular traffic and industrial activities on PM air pollution, including adopting holistic source-control measures for improved air quality in urban environments.
Minimizing Contamination from Plastic Labware in the Quantification of C16 and C18 Fatty Acids in Filter Samples of Atmospheric Particulate Matter and Their Utility in Apportioning Cooking Source Contribution to Urban PM2.5
Authors: Cheng Yuk Ying , Yu Jian Zhen
Published: October 2020

Palmitic acid (C16:0) and stearic acid (C18:0) are among the most abundant products in cooking emission, and thus could serve as potential molecular tracers in estimating the contributions of cooking emission to particulate matter (PM2.5) pollution in the atmosphere. Organic tracer analysis in filter-based samples generally involves extraction by organic solvents, followed by filtration. In these procedures, disposable plastic labware is commonly used due to convenience and as a precaution against sample-to-sample cross contamination. However, we observed contamination for both C16:0 and C18:0 fatty acids, their levels reaching 6–8 ppm in method blanks and leading to their detection in 9% and 42% of PM2.5 samples from Hong Kong, indistinguishable from the blank. We present in this work the identification of plastic syringe and plastic syringe filter disc as the contamination sources. We further demonstrated that a new method procedure using glass syringe and stainless-steel syringe filter holder offers a successful solution. The new method has reduced the contamination level from 6.6 ± 1.2 to 2.6 ± 0.9 ppm for C16:0 and from 8.9 ± 2.1 to 1.9 ± 0.8 ppm for C18:0 fatty acid. Consequently, the limit of detection (LOD) for C16:0 has decreased by 57% from 1.8 to 0.8 ppm and 56% for C18:0 fatty acid from 3.2 to 1.4 ppm. Reductions in both LOD and blank variability has allowed the increase in quantification rate of the two fatty acids in ambient samples and thereby retrieving more data for estimating the contribution of cooking emission to ambient PM2.5. With the assistance of three cooking related tracers, palmitic acid (C16:0), stearic acid (C18:0) and cholesterol, positive matrix factorization analysis of a dataset of PM2.5 samples collected from urban Hong Kong resolved a cooking emission source. The results indicate that cooking was a significant local PM2.5 source, contributing to an average of 2.2 µgC/m3 (19%) to organic carbon at a busy downtown roadside location and 1.8 µgC/m3 (15%) at a general urban site.
Differentiation of the Athens Fine PM Profile during Economic Recession (March of 2008 Versus March of 2013): Impact of Changes in Anthropogenic Emissions and the Associated Health Effect
Authors: Pateraki Styliani , Fameli Kyriaki-Maria , Assimakopoulos Vasiliki , Bairachtari Kyriaki , Zagkos Alexandros , Stavraka Theodora , Bougiatioti Aikaterini , Maggos Thomas , Mihalopoulos Nikolaos
Published: October 2020

Despite the various reduction policies that have been implemented across Europe in the past few years, Particulate Matter (PM) exceedances continue to be recorded. Therefore, with the principal aim to clarify the complex association between emissions and fine particles levels, this work evaluates the impact of the anthropogenic contribution to the fine PM chemical profile. The fieldwork was conducted during March in 2008 and 2013 and covers the periods before and during the economic recession. The experimental data were analyzed in parallel with the emissions from the Flexible Emission Inventory for Greece and the Greater Athens Area (FEI-GREGAA). The differentiation of the mass closure results’ and the aerosols’ character is also discussed in combination with the calculated PM2.5-Air Quality Indexes. The peak in the PM load and the Particulate Organic Matter (POM) component was recorded in 2013, corresponding to the enhancement of the anthropogenic input. Although the monitoring location is traffic-impacted, the sector of heating, from both wood burning and fossil fuel, proved to be the driving force for the configuration of the obtained PM picture. Especially in 2013, its contribution was two times that of traffic. Finally, the low wind speed values led to the deterioration of the air quality, especially for the sensitive groups.
Treatment of Bad Big Data in Research Data Management (RDM) Systems
Authors: Azeroual Otmane
Published: October 2020

Databases such as research data management systems (RDMS) provide the research data in which information is to be searched for. They provide techniques with which even large amounts of data can be evaluated efficiently. This includes the management of research data and the optimization of access to this data, especially if it cannot be fully loaded into the main memory. They also provide methods for grouping and sorting and optimize requests that are made to them so that they can be processed efficiently even when accessing large amounts of data. Research data offer one thing above all: the opportunity to generate valuable knowledge. The quality of research data is of primary importance for this. Only flawless research data can deliver reliable, beneficial results and enable sound decision-making. Correct, complete and up-to-date research data are therefore essential for successful operational processes. Wrong decisions and inefficiencies in day-to-day operations are only the tip of the iceberg, since the problems with poor data quality span various areas and weaken entire university processes. Therefore, this paper addresses the problems of data quality in the context of RDMS and tries to shed light on the solution for ensuring data quality and to show a way to fix the dirty research data that arise during its integration before it has a negative impact on business success.
The Macro- and Micro-Mechanics of the Colon and Rectum I: Experimental Evidence
Authors: Siri Saeed , Zhao Yunmei , Maier Franz , Pierce David M. , Feng Bin
Published: October 2020

Many lower gastrointestinal diseases are associated with altered mechanical movement and deformation of the large intestine, i.e., the colon and rectum. The leading reason for patients’ visits to gastrointestinal clinics is visceral pain, which is reliably evoked by mechanical distension rather than non-mechanical stimuli such as inflammation or heating. The macroscopic biomechanics of the large intestine were characterized by mechanical tests and the microscopic by imaging the load-bearing constituents, i.e., intestinal collagen and muscle fibers. Regions with high mechanical stresses in the large intestine (submucosa and muscularis propria) coincide with locations of submucosal and myenteric neural plexuses, indicating a functional interaction between intestinal structural biomechanics and enteric neurons. In this review, we systematically summarized experimental evidence on the macro- and micro-scale biomechanics of the colon and rectum in both health and disease. We reviewed the heterogeneous mechanical properties of the colon and rectum and surveyed the imaging methods applied to characterize collagen fibers in the intestinal wall. We also discussed the presence of extrinsic and intrinsic neural tissues within different layers of the colon and rectum. This review provides a foundation for further advancements in intestinal biomechanics by synergistically studying the interplay between tissue biomechanics and enteric neurons.
Differential Production of Phenolics, Lipids, Carbohydrates and Proteins in Stressed and Unstressed Aquatic Plants, Azolla filiculoides and Azolla pinnata
Authors: Tran Thi Linh Nham , Miranda Ana F. , Abeynayake Shamila Weerakoon , Mouradov Aidyn
Published: October 2020

The metabolic plasticity of shikimate and phenylpropanoid pathways redirects carbon flow to different sink products in order to protect sessile plants from environmental stresses. This study assessed the biochemical responses of two Azolla species, A. filiculoides and A. pinnata, to the combined effects of environmental and nutritional stresses experienced while growing outdoors under Australian summer conditions. These stresses triggered a more than 2-fold increase in the production of total phenols and their representatives, anthocyanins (up to 18-fold), flavonoids (up to 4.7-fold), and condensed tannins (up to 2.7-fold), which led to intense red coloration of the leaves. These changes were also associated with an increase in the concentration of carbohydrates and a decrease in concentrations of lipids and total proteins. Changes in lipid biosynthesis did not cause significant changes in concentrations of palmitoleic acid (C16:0), linolenic acid (C18:3), and linoleic acid (C18:2), the fatty acid signatures of Azolla species. However, a reduction in protein production triggered changes in biosynthesis of alanine, arginine, leucine, tyrosine, threonine, valine, and methionine amino acids. Stress-triggered changes in key nutritional components, phenolics, lipids, proteins, and carbohydrates could have a significant impact on the nutritional value of both Azolla species, which are widely used as a sustainable food supplement for livestock, poultry, and fish industries.
A Facile Approach for Rapid Prototyping of Microneedle Molds, Microwells and Micro-Through-Holes in Various Substrate Materials Using CO2 Laser Drilling
Authors: Chen Yu-Wei , Chen Mei-Chin , Wu Kuang-Wei , Tu Ting-Yuan
Published: October 2020

CO2 laser manufacturing has served as an enabling and reliable tool for rapid and cost-effective microfabrication over the past few decades. While a wide range of industrial and biological applications have been studied, the choice of materials fabricated across various laser parameters and systems is often confounded by their complex combinations. We herein presented a unified procedure performed using percussion CO2 laser drilling with a range of laser parameters, substrate materials and various generated microstructures, enabling a variety of downstream tissue/cellular-based applications. Emphasis is placed on delineating the laser drilling effect on different biocompatible materials and proof-of-concept utilities. First, a polydimethylsiloxane (PDMS) microneedle (MN) array mold is fabricated to generate dissolvable polyvinylpyrrolidone/polyvinyl alcohol (PVP/PVA) MNs for transdermal drug delivery. Second, polystyrene (PS) microwells are optimized in a compact array for the formation of size-controlled multicellular tumor spheroids (MCTSs). Third, coverglass is perforated to form a microaperture that can be used to trap/position cells/spheroids. Fourth, the creation of through-holes in PS is validated as an accessible method to create channels that facilitate medium exchange in hanging drop arrays and as a conducive tool for the growth and drug screenings of MCTSs.
Differences in Tolerance to Hypoxia: Physiological, Biochemical, and Molecular-Biological Characteristics
Authors: Dzhalilova Dzhuliia , Makarova Olga
Published: October 2020

Hypoxia plays an important role in the development of many infectious, inflammatory, and tumor diseases. The predisposition to such disorders is mostly provided by differences in basic tolerance to oxygen deficiency, which we discuss in this review. Except the direct exposure of different-severity hypoxia in decompression chambers or in highland conditions, there are no alternative methods for determining organism tolerance. Due to the variability of the detection methods, differences in many parameters between tolerant and susceptible organisms are still not well-characterized, but some of them can serve as biomarkers of susceptibility to hypoxia. At the moment, several potential biomarkers in conditions after hypoxic exposure have been identified both in experimental animals and humans. The main potential biomarkers are Hypoxia-Inducible Factor (HIF)-1, Heat-Shock Protein 70 (HSP70), and NO. Due to the different mechanisms of various high-altitude diseases, biomarkers may not be highly specific and universal. Therefore, it is extremely important to conduct research on hypoxia susceptibility biomarkers. Moreover, it is important to develop a method for the evaluation of organisms’ basic hypoxia tolerance without the necessity of any oxygen deficiency exposure. This can contribute to new personalized medicine approaches’ development for diagnostics and the treatment of inflammatory and tumor diseases, taking into account hypoxia tolerance differences.
Sex-Dependent Changes in Right Ventricular Gene Expression in Response to Pressure Overload in a Rat Model of Pulmonary Trunk Banding
Authors: Labazi Hicham , Axelsen Julie Birkmose , Hillyard Dianne , Nilsen Margaret , Andersen Asger , MacLean Margaret R.
Published: October 2020

Right ventricular hypertrophy (RVH) and subsequent failure are consequences of pulmonary arterial hypertension (PAH). While females are four times more likely to develop PAH, male patients have poorer survival even with treatment, suggesting a sex-dependent dimorphism in right ventricular (RV) hypertrophy/compensation. This may result from differential gene expression in the RV in male vs. female. To date, the sex dependent effect of pressure overload on RV function and changes in gene expression is still unclear. We hypothesize that pressure overload promotes gene expression changes in the RV that may contribute to a poorer outcome in males vs. females. To test this hypothesis, male and female Wistar rats underwent either a sham procedure (sham controls) or moderate pulmonary trunk banding (PTB) (a model of pressure overload induced compensated RV hypertrophy) surgery. Seven weeks post-surgery, RV function was assessed in vivo, and tissue samples were collected for gene expression using qPCR. Compared to sham controls, PTB induced significant increases in the right ventricular systolic pressure, the filling pressure and contractility, which were similar between male and female rats. PTB resulted in an increase in RVH indexes (RV weight, RV weight/tibia length and Fulton index) in both male and female groups. However, RVH indexes were significantly higher in male-PTB when compared to female-PTB rats. Whilst end of procedure body weight was greater in male rats, end of procedure pulmonary artery (PA) diameters were the same in both males and females. RV gene expression analysis revealed that the following genes were increased in PTB-male rats compared with the sham-operated controls: natriuretic peptide A (ANP) and B (BNP), as well as the markers of fibrosis; collagen type I and III. In females, only BNP was significantly increased in the RV when compared to the sham-operated female rats. Furthermore, ANP, BNP and collagen III were significantly higher in the RV from PTB-males when compared to RV from PTB-female rats. Our data suggest that pressure overload-mediated changes in gene expression in the RV from male rats may worsen RVH and increase the susceptibility of males to a poorer outcome when compared to females.
Inhibition of Renal Stellate Cell Activation Reduces Renal Fibrosis
Authors: Cha Jin Joo , Mandal Chanchal , Ghee Jung Yeon , Yoo Ji Ae , Lee Mi Jin , Kang Young Sun , Hyun Young Youl , Lee Ji Eun , Kim Hyun Wook , Han Sang Youb , Han Jee Young , Chung Ah Young , Yoon Dae Wui , Rhyu Im Joo , Oh Junseo , Cha Dae Ryong
Published: October 2020

Interstitial fibrosis is a common feature of chronic kidney disease, and platelet-derived growth factor receptor-β (PDGFR-β)-positive mesenchymal cells are reportedly the major source of scar-producing myofibroblasts. We had previously demonstrated that albumin and its derivative R-III (a retinol-binding protein-albumin domain III fusion protein) inhibited the transdifferentiation/activation of hepatic stellate cells (HSCs) to myofibroblasts and that R-III administration reduced liver fibrosis. In this study, we isolated cells (referred to as renal stellate cells, RSCs) from rat kidney tissues using the HSC isolation protocol and compared their morphological and biochemical characteristics with those of HSCs. RSCs shared many characteristics with HSCs, such as storage of vitamin A-containing lipid droplets and expression of HSC markers as well as pericyte markers. RSCs underwent spontaneous transdifferentiation into myofibroblasts in in vitro culture, which was inhibited by albumin expression or R-III treatment. We also evaluated the therapeutic effects of R-III in unilateral ureteral obstruction (UUO)-induced renal fibrosis in mice. Injected R-III localized predominantly in cytoglobin/stellate cell activation-associated protein (Cygb/STAP)-positive cells in the kidney and reduced renal fibrosis. These findings suggest that RSCs can be recognized as the renal counterparts of HSCs and that RSCs represent an attractive therapeutic target for anti-fibrotic therapy.
Gold Nanorods for LSPR Biosensing: Synthesis, Coating by Silica, and Bioanalytical Applications
Authors: Pellas Vincent , Hu David , Mazouzi Yacine , Mimoun Yoan , Blanchard Juliette , Guibert Clément , Salmain Michèle , Boujday Souhir
Published: October 2020

Nanoparticles made of coinage metals are well known to display unique optical properties stemming from the localized surface plasmon resonance (LSPR) phenomenon, allowing their use as transducers in various biosensing configurations. While most of the reports initially dealt with spherical gold nanoparticles owing to their ease of synthesis, the interest in gold nanorods (AuNR) as plasmonic biosensors is rising steadily. These anisotropic nanoparticles exhibit, on top of the LSPR band in the blue range common with spherical nanoparticles, a longitudinal LSPR band, in all respects superior, and in particular in terms of sensitivity to the surrounding media and LSPR-biosensing. However, AuNRs synthesis and their further functionalization are less straightforward and require thorough processing. In this paper, we intend to give an up-to-date overview of gold nanorods in LSPR biosensing, starting from a critical review of the recent findings on AuNR synthesis and the main challenges related to it. We further highlight the various strategies set up to coat AuNR with a silica shell of controlled thickness and porosity compatible with LSPR-biosensing. Then, we provide a survey of the methods employed to attach various bioreceptors to AuNR. Finally, the most representative examples of AuNR-based LSPR biosensors are reviewed with a focus put on their analytical performances.
Understanding Novel Metaphors: A Milestone in the Developmental Trajectory of Children with Agenesis of the Corpus Callosum?
Authors: Melogno Sergio , Pinto Maria Antonietta , Pollice Chiara , Badolato Fausto , Trasimeni Guido , Parisi Pasquale
Published: October 2020

This study explores novel metaphor comprehension in a 7.2-year-old child (conventionally called RJ) with complete and isolated agenesis of the corpus callosum (ACC). RJ’s cognitive level was adequate for his age as well as most of his linguistic competencies. The child’s performance was compared to typically developing (TD) controls on a test assessing novel metaphor comprehension for preschoolers. RJ’s performance showed a delay of about three years in relation to the expected level for his age, and also a significant gap compared to the TDs. The results highlighted the possibility to detect weaknesses in understanding novel metaphors in children with ACC, in spite of their apparently adequate linguistic capabilities. An early detection of a weakness in this area can pave the way to neurolinguistic treatment in order to enhance the understanding of nonliteral meaning, which, in the developmental trajectory, will be increasingly involved in everyday life communication. Future research should explore more in-depth a capability that intrinsically requires high interconnectivity, such as novel metaphor comprehension, in a brain in development where the major tract connecting the two hemispheres is missing.