bluuebunny/crossref_metadata_2025 huggingface dataset:
Combining bioinformatics and network pharmacology to explore diagnostic biomarkers and mechanisms associated with mitochondria in diabetic nephropathy using Rhodiola Rosea
Authors: Wang Ruihua , Yang XiuJie , Fang Jingai , Zhang Ziyuan , Hu Yaling , Wang Xiaoyu , Guo Yuanyuan , Yang Runan
Published: July 2024

This study aimed to explore diagnostic biomarkers associated with mitochondria in the treatment of diabetic nephropathy (DN) using Rhodiola Rosea (RR) and to elucidate the underlying regulatory mechanisms. The target genes corresponding to the active ingredients of RR, mitochondria-related genes, and DN-related genes were intersected with differentially expressed genes from GSE96804 to obtain mitochondria-related pharmacopoeia genes (MRPGs). Based on these genes, a pharmacological network was constructed. Additionally, the biological pathways enriched by the diagnostic biomarkers, their correlation with the DN immune infiltration profile, and their binding ability to RR were analyzed. A total of 24 MRPGs against DN were identified using Venn analysis. Subsequently, six diagnostic biomarkers were screened by machine learning, namely CAT, CASP3, CPT1A, OGDH, CYP27B1, and ALDH9A1. Immune infiltration analysis showed eight differential immune cells between the DN and control groups, with macrophages M2 and neutrophils correlating with all diagnostic biomarkers. Based on the pharmacological network and molecular docking, two targeting relationship pairs were identified: CAT-RUTIN and ALDH9A1-RUTIN-2, both demonstrating good binding capacity. This study identified six diagnostic biomarkers for DN based on the effects of RR and mitochondrial function. The findings revealed the active components and potential molecular therapeutic mechanisms of RR in treating DN.
Rearrangement of anti-synchronous activities in the brain functional network plays a crucial role in behavioral contagion
Authors: Mobasseri Mohsen , Vahabie Abdol-Hossein , Jafari Gholamreza , Hatami Javad , Khosrowabadi Reza
Published: July 2024

Behavioral contagion has been defined as the tendency of individuals to imitate the behavior of others after observing them. Despite the important role that behavioral contagion plays in societies, its mechanism in the brain is still not fully understood. In this study, we hypothesized that the brain tends to go to a more stable state after updating behavior by observation of the others’ behaviors. Therefore, the stability of the brain network before and after observing others’ preferences was assessed using structural balance theory (SBT) on the fMRI data. To this end, we developed a version of the Dictator Game as the task, and recorded participants' brain responses using fMRI (before and after observing others' preferences). A threshold for changes in participants' preferences was considered to be the occurrence of behavioral contagion. With regard to this threshold, the participants were classified into two groups, the Contagion and No Contagion. The changes in SBT parameters of the brain network were calculated for both groups. A distinct pattern of changes in SBT parameters was observed for each group. The results of the Contagion group suggested that behavioral contagion is accompanied with a rearrangement of links in the network to transform imbalanced triads into balanced triads. This process lowers the balance energy of the brain network and pushes the network to a more stable state. We hope that these findings on the restructuring of the functional brain network could pave the way to a better understanding of behavioral contagion.
Harmonization of Longitudinal Diffusion Tensor Imaging Data of the Pediatric Cervical and Thoracic Spinal Cord at 3T Using Longitudinal ComBat
Authors: Li Yutong , Middleton Devon M , Chen Andrew , Shinohara Russell T , Krisa Laura , Faro Scott H , Mulcahey Mary Jane , Mohamed Feroze B
Published: July 2024

Diffusion tensor imaging (DTI) of the spinal cord has been extensively used to identify biomarkers for spinal cord pathology. Previously, the longitudinal ComBat (longComBat) technique was examined to reduce scanner effects in multi-site, multi-scanner spinal cord DTI data. This study aimed to assess its effectiveness on longitudinal scans using a single-scanner pediatric dataset, including healthy and spinal cord injury (SCI) subjects. Two identical datasets were collected from 42 healthy and 27 SCI subjects with a 2-hour interval between scans on a 3T Siemens MRI scanner. Axial DTI images of the entire cervical and thoracic spinal cord were obtained, and various average diffusion tensor metrics (FA, MD, RD, & AD) were measured at each vertebral level. Pearson correlation and intraclass correlation coefficients were used to evaluate inter- and intra-subject agreement pre- and post-harmonization. Minimal improvement in agreement was observed with the mean square residual (MSR) model, while the restricted maximum likelihood estimator (REML) model showed reduced intra-subject agreement in all the tensor metrics. The significant variability between longitudinal DTI scans within a single scanner was likely due to physiological motion rather than scanner effects. Post-harmonization using the longComBat MSR model showed limited improvement in agreement.
Green synthesis of silver nanoparticles (Ag) using Dracaena Sanderiana’s leaves extract and its structural, morphological, and antimicrobial study
Authors: Raikwar Vaishali , Jaiswar Vivek , Patil Vinayak , Golwalkar Sucheta
Published: July 2024

Silver (Ag) nanoparticles are one of the most studied and advanced functional materials. They are the potential candidates in almost every field of life. The useful properties are enhanced in various applications like sensors, catalysis, and antibacterial activity. The method of synthesis has a significant effect on the size and shape of silver nanoparticles. The green synthesis of silver nanoparticles using natural products has been an excellent solution for chemically derived silver nanoparticles. Herein, a rapid, process has been established using an extract from leaves of Dracaena Sanderiana (Lucky bamboo) plant, for the synthesis of metallic silver nanoparticles (Ag NP) for the first time. The synthesized Ag nanoparticles were characterized by X-ray diffraction (XRD), UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The agar cup diffusion method was used to check the antimicrobial effect of Ag NPs on different microorganisms with a zone of inhibition of 21 mm for gram-positive Staphylococcus aureus and 18 mm for gram-negative E. coli when 50 µg of Ag NPs were used. Thus, the silver nanoparticles are excellent antimicrobial agent.
Spinal Chordoma and Chondrosarcoma Treatment Experiences - A 20-Year Retrospective Study from Databases of Two Medical Centers
Authors: Kuo Pin-Lin , Yeh Yu-Cheng , Chang Koping , Tsai Tsung-Ting , Lai Po-Liang , Tsuang Fon-Yih
Published: July 2024

The research retrospectively analyzed cases of spinal chordoma and chondrosarcoma involving patients who received treatment at the two hospitals between 2001 and 2023. Among the 48 patients studied (39 chordoma and 9 chondrosarcoma cases), the average age was 53.9 ± 15.8 years, with a range of 17 to 86 years. Out of these patients, 43 underwent excision surgery and were categorized based on tumor margin into negative (R0) or microscopically positive (R1) margin (n = 14) and macroscopically positive (R2) margin (n = 29) groups. The mean overall survival (OS) for R0/R1 and R2 groups was 156.5 ± 19.3 and 79.2 ± 11.9 months, respectively (p value = 0.012). The mean progression-free survival (PFS) for R0/R1 and R2 was 112.9 ± 24.4 and 25.5 ± 5.5 months (p value < 0.001). The study showed that regardless of whether patients in the R0/R1 or R2 groups received radiation therapy (RT) or not, there was no significant improvement in OS or PFS. Specifically, the OS and PFS for the RT only group were 75.9 ± 16.6 and 73.3 ± 18.0 months. In conclusion, the recommended treatment approach for spinal chordoma and chondrosarcoma remains en bloc resection surgery with an appropriate margin. Patients who are unsuitable for or decline surgery may find a beneficial disease control rate with traditional external beam photon/proton therapy.
Factors associated with physical activity among Thai women with breast cancer postmastectomy at Siriraj Hospital, Thailand
Authors: Khobkhun Fuengfa , Wichaidit Piyaporn , Sa-nguanraksa Doonyapat , Phumphuang Surat , Puranasamriddhi Saowaluck , Lohsiriwat Visnu , Kaewkhuntee Watesinee
Published: July 2024

Background
The primary treatment for breast cancer patients is surgery, with the aim of reducing the risk of recurrence and minimize the spread of cancer cells. However, breast surgery often impacts physical activity (PA). Therefore, this study aimed to investigate factors associated with the PA level of Thai women with breast cancer after surgical mastectomy at Siriraj Hospital, Thailand.
Methods
Ninety-three participants were completed the questionnaires to accumulate the data for this study included personal information, information regarding medical treatment for breast cancer, the Global Physical Activity Questionnaire, perceived benefits, perceived barriers, perceived self-efficacy, interpersonal influence and situational influence, the Thai Body Image Index, and the Thai Hospital Anxiety and Depression Scale. Data was analyzed using descriptive statistics and Chi-square statistics (Odds-Ratio (OR) and 95% Confidence Interval (CI)).
Results
The study found that after post-mastectomy Thai women had sufficient PA (2.8 metabolic equivalent of tasks (METs)). It was also found that PA at work was associated with occupation, higher incomes, moderate satisfaction as assessed by the Thai Body Image Index, and higher levels of anxiety and depression. Additionally, participation in recreational activities was associated with older age, higher perceived self-efficacy, and greater interpersonal influence.
Conclusion
Understanding the factors associated with PA in Thai women with breast cancer postmastectomy is essential for developing comprehensive interventions that optimize patient health and well-being throughout the cancer journey. By exploring these connections more deeply, we can tailor supportive care strategies that address the holistic needs of individuals affected by breast cancer.
Estrogen Receptor Expression Pattern in Follicular Thyroid Cancers: A 23 Years Retrospective Multi-Centre Study in Jos Metropolis, North-Central, Nigeria.
Authors: Richard Samuel Kelechi , Kwaghe Barka Vandi , Emmanuel Innocent , Akpa Philip Ojile , Jimoh Abdulrazaq Ajanaku
Published: July 2024

Background
Follicular Thyroid cancers (FTC) are the second commonest malignancy of this organ and seen more often in females. Estrogen Receptor (ER) is vital in its pathogenesis and prognosis and those that express this marker may respond to selective medical targeted therapy. The aim of the study is to establish ER status of FTC as seen during the study period and this will give insight into possible response to medical targeted therapy.
Methods
This was a hospital based, retrospective multicenter study. The biodata were obtained from surgical pathology cancer registries. Sections from respective tissue blocks of included cases were reviewed, classified, subjected to immunohistochemistry, scored (using Allred) and analyzed using SPSS-20. Descriptive statistics was used to determine frequencies of each categorical variable, mean and mode of continuous variable.
Results
A total of 34 (36%) out of 95 cases of thyroid cancers were included. The mean, mode, median and age range were 49.8 ± 15.4, 44.5, 42 and 32–70 years respectively. Females were predominant (64.7%) with minimal invasive commonest. Three cases (8.8%) were only positive for ER out of which all (100.0%) were females. Two of the positive cases had score 3(5.9%) while 1 had score 5(2.9%).
Conclusions
Majority of the follicular thyroid cancer cases were negative for estrogen receptors and were seen exclusively in female of reproductive age group.
Investigation the role of SIRT3, SIRT7, NFATC1, and PDL-1 genes in Androgenetic Alopecia
Authors: Abbasian Hadis , Noruzinia Mehrdad , Garshasbi Masoud
Published: July 2024

Background Androgenetic Alopecia (AGA) stands as the most prevalent form of hair loss, affecting the hair follicles (HFs). aging emerges as a prominent contributor in this condition. In this study, our aim is to elucidate the expression patterns of candidate genes—SIRT3, SIRT7, NFATC1, and PDL-1—known to exhibit differential expression levels during HF aging, and to underscore the role of aging in AGA.Material and Methods mesenchymal stem cells (MSCs) were isolated from the vertex and occipital regions of six men affected by AGA. The aim was to assess the expression levels of SIRT3, SIRT7, NFATC1, and PDL-1 genes. RNA extraction was performed followed by cDNA synthesis, and gene expression levels were quantified using real-time PCR. To validate the experimental findings, two different RNA-seq datasets relevant to the study were analyzed using R software.Results In the present study, experimental tests revealed that the expression levels of SIRT3 and SIRT7, known to decrease during HF aging, were diminished in AGA samples as well. Conversely, the mean value of NFATC1 and PDL-1 expression level, which are known to increase during HF aging, were found to be elevated in AGA samples. Moreover, bioinformatic analyses provide additional support for the role of SIRT3, SIRT7, NFATC1, and PDL-1 in AGA pathogenesis.Conclusion while SIRT3 and SIRT7 may play critical roles in AGA development, further research is needed to elucidate the significance of NFATC1 and PDL-1 in this context and to explore their potential as therapeutic targets for AGA treatment.
General Endotracheal Anesthesia vs Total Intravenous Anesthesia for Children Undergoing Low-risk Cardiac Catheterization
Authors: Fashina Oluwatomini , Vogel Elizabeth , Swan Elena Ashikhmina , Anderson Jason , Aganga Devon , Gleich Stephen , Stensrud Paul , Taggart Nathaniel
Published: July 2024

Introduction:
Historically, children undergoing cardiac catheterization have been managed with general endotracheal anesthesia (GETA). However, recent practice has favored general anesthesia with total intravenous anesthesia (TIVA). This study compares procedural outcomes in the pediatric cardiac catheterization laboratory (PCCL) for children undergoing low-risk cardiac catheterization with GETA vs. TIVA.
Methods:
We reviewed all patients aged 1–12 years, who underwent outpatient diagnostic or simple interventional catheterization from January 2016 – September 2023. Patients were divided into cohorts by type of anesthesia (GETA vs. TIVA). The primary outcome measure was total patient time in the PCCL (“door-to-door” time). Secondary outcomes were procedure duration (“sheath time”), length of hospital stay, and the major adverse event (MAE) rate.
Results:
A total of 401 patients were included. Of these patients, 226 underwent GETA and 175 underwent TIVA. There were 148 interventional procedures and 72 patients had single-ventricle physiology. There was no significant difference between the two cohorts in terms of demographic factors such as age, weight, procedure indication and physiological complexity as well as outcomes such as sheath time, hospital stay and MAE. However, the average door-to-door time of the TIVA cohort was 25 minutes shorter than the GETA cohort (p < 0.001).
Conclusions:
In this single center, retrospective study of anesthesia management for children undergoing low-risk cardiac catheterization, TIVA is associated with significantly shorter door-to-door time in the PCCL without an increase in procedure duration, hospital stay, or rate of complications. These findings have potential clinical significance supporting increased use of TIVA during pediatric cardiac catheterization.
The Intelligent Selenium-Enriched Tea WitheringControl System
Authors: Ren Shuang , Fan Jinzhao , Lu Didi , Liu Sanjun
Published: July 2024

This paper addresses the low level of intelligence in tea processing equipment in Enshi Prefecture by designing an intelligentwithering control system based on the STMicroelectronics 32-bit Microcontroller (STM32). This control system can achievereal-time monitoring of the withering environment and automate the control of heating and ventilation dehumidification modules.By integrating IoT technology, relevant users can view the tea production process via mobile devices, enabling intelligent andremote production operations. Application results show that the system operates stably, accurately measures temperature andhumidity in the withering environment, and achieves a control precision of ±1% through a fuzzy control algorithm. It effectivelymeets the needs of tea processing in Enshi Prefecture. The system not only optimizes traditional processing workflows,enhancing processing efficiency and tea quality, but also provides new technological means for tea processing enterprises,contributing to the development and upgrading of the local tea industry.
Stenotrophomonas maltophilia endogenous endophthalmitis in pediatric age group
Authors: Jain Tanya , Muralidhar Alankrita , Gandhi Arpan
Published: July 2024

Background: We report a unique case of Stenotrophomonas maltophilia-related pediatric endogenous endophthalmitis.
Case presentation: A 10-year-old male presented with redness and loss of vision in his right eye for two weeks. Clinical examination and ultrasound features were suggestive of endophthalmitis, most likely endogenous due to the absence of a history of trauma or intraocular intervention. Following vitrectomy and appropriate intravitreal antibiotics, the vision improved to 20/80. Vitreous culture revealed a gram-negative bacillus Stenotrophomonas maltophilia.
Conclusion: Timely recognition of this pathogen and management as per antibiotic sensitivity can help salvage functional vision in this condition.
Breastfeeding with Primary Low Milk Supply: A Phenomenological Exploration of Mother’s Lived Experiences of Professional and Peer Support
Authors: Whelan Caoimhe , O'Brien Denise , Hyde Abbey
Published: July 2024

Background: Most women can produce enough milk to exclusively breastfeed. However, a small cohort are prevented from doing so due to a condition known as primary low milk supply.
Aim: To provide new insights into how mothers with primary low milk supply experience help and support from professionals, volunteers, and partners.
Methods: Interpretative phenomenological analysis was used to explore the lived experiences of nine breastfeeding mothers with primary low milk supply. Hour-long interviews were conducted over Zoom.
Results: Being with Others is one of four superordinate themes identified in this study. This theme concerned the participants’ experiences of receiving professional, peer, and voluntary breastfeeding supports. It also considers how participants were supported by their partners. Four sub-themes were identified: Disconnected Encounters, Perceiving Judgement from Others, Being in a Safe Space and Having a Saviour.
Conclusion: The interactions that mothers with primary low milk supply have with various others in their world (health care professionals, lactation consultants, peer breastfeeding supporters, and partners) can shape how they view themselves and can have a profound impact on their breastfeeding journey. The encounters of participants with healthcare professionals revealed a lack of rapport, intrusive hands-on support, poor perceived quality of breastfeeding support and a heightened sensitivity to language and tone. Mothers with primary low milk supply value empathy and emotional support from lactation professionals as much as they value skilled lactation support. We recommend that healthcare professionals receive education concerning primary low milk supply and communication skills training on the provision of sensitive person-centred breastfeeding support.
Ultrastructure of disc found in the chorion of Atlantic salmon (Salmo salar) embryos
Authors: Pérez-Atehortúa Maritza , Sandoval-Vargas Leydy , Risopatrón Jennie , Farías Jorge , Villalobos Elías Figueroa , Valdebenito Iván
Published: July 2024

The chorion is an acellular envelope that surrounds fish oocytes and embryos, playing a crucial role in protection against environmental factors until hatching. In salmonids, several chorion malformations have been documented, including white-spotted (disc-like) chorion. This study focused on investigating the ultrastructural modifications of the chorion with discs and their possible influence on embryonic viability in Atlantic salmon. A total of 136 females were included in the study. The incidence of disc chorion was evaluated at 130 °h and in pigmented eye embryos at 280 ATU (accumulated thermal units). Scanning electron microscopy (SEM) was used for this ultrastructural characterization. The results revealed that 3.17% of females had embryos with discs in the chorion at 130 °h, with a mean of 1.21 ± 7.24% of affected embryos per female. At 280 ATU, 15% of the females had embryos with discs in the chorion, with a mean of 0.45 ± 2.17% per female. Females with this malformation were observed to have a slightly higher fertilization rate (75 ± 21.21%) compared to females without this malformation (74.2 ± 23.7%). Embryonic survival at 280 ATU for both groups was 70.1 ± 20.6% and 71.6 ± 21.0%, respectively. Although a clear origin for the chorion with disc malformation is not known, the possibility is raised that it may be due to pathological conditions specific to the female and that it probably occurs in a similar way to nephrocalcinosis, by bioaccumulation of elements in the chorion during its synthesis. Further studies are needed to better understand this malformation to help refine economic projections in salmon farms dedicated to embryo production.
Fine-Grained Meetup Events Extraction Through Context-Aware Event Argument Positioning and Recognition
Authors: Lin Yuan-Hao , Chang Chia-Hui , Chuang Hsiu-Min
Published: July 2024

Extracting meetup events from social network posts or webpage announcements is the core technology to build event search services on the Web. While event extraction in English achieves good performance in sentence-level evaluation WKGS19,the quality of auto-labeled training data via distant supervision is not good enough for word-level event extraction due to long event titles JISE2022.Additionally, meetup event titles are more complex and diverse than trigger-word-based event extraction. Therefore, the performance of event title extraction is usually worse than that of traditional named entity recognition.In this paper, we propose a context-aware meetup event extraction (CAMEE) framework that incorporates a sentence-level event argument positioning model to locate event fields (i.e., title, venue, dates, etc.) within a message and then perform word-level event title, venue, and date extraction.Experimental results show that adding sentence-level event argument positioning as a filtering step improves the word-level event field extraction performance from 0.726 to 0.743 macro-F1, outperforming large language models like GPT-4-turbo (with 0.549 F1) and SOTA NER model SoftLexicon (with 0.733 F1). If we focus on the main event, the proposed model achieves 0.784 macro-F1.
Nisin A-producing Lactococcus cremoris formulations for pre- and post-milking teat disinfection modulate the bovine milk microbiota
Authors: Gazzola Alessandra , Ceccarani Camilla , Castiglioni Bianca , Biscarini Filippo , Morandi Stefano , silvetti tiziana , piccinini renata , Brasca Milena , Cremonesi Paola
Published: July 2024

Background - Bovine mastitis is a major challenge in dairy farms. Since the agents commonly used for pre- and post-dipping can affect the udder health by modifying milk microbiota, alternative products are needed. This study aimed to evaluate the effect of the use of pre- and post-dipping formulations containing the Nisin A-producing Lactococcus cremoris FT27 strain (treated group, TR) on the abundance and biodiversity of milk microbiota as compared to iodine-based commercial disinfectants (control group, CTR) during a three-month trial. The experiment was conducted on 20 dairy cows, divided into two groups (CTR and TR) of 10 lactating cows each. Milk samples were collected from two selected healthy quarters of each cow at 3 time points. Microbial communities were investigated by cultural and sequence-based methods, and analyzed through bioinformatic and statistical approaches. Results - Clear differences in bacterial community composition were observed among groups, with higher species richness in TR, especially of Staphylococcus, Enterococcus, Lactococcus, and Streptococcus genera. The microbiota was dominated by Firmicutes, followed by Actinobacteriota, Proteobacteria, and Bacteroidota. Staphylococcaceae family was significantly higher in TR (p < 0.009), whereas Carnobacteriaceae, Mycobacteriaceae, and Pseudomonadaceae were significantly lower (p = 0.005, p = 0.001, and p = 0.040, respectively). CTR had considerably higher abundances of the genera Alkalibacterium (p = 0.011), Pseudomonas_E (p = 0.045), Corynebacterium (p = 0.004), and Alloiococcus (p = 0.004), and lower abundances of Staphylococcus (p < 0.009). Milk microbiota changed noticeably during the experimental period, regardless of treatment. A significant decrease was observed in both groups for Firmicutes_A phylum, with an increment in Actinobacteriota phylum, Propionibacteriaceae family, and Cutibacterium genus. Streptococcaceae significantly decreased in CTR (p = 0.013) and rose in TR (p = 0.001). Several differences were observed between the two groups during the experimental period. Streptococcus genus almost disappeared in CTR (p = 0.013), whereas it significantly increased in TR (p = 0.001). Three and twelve enriched groups were significantly identified respectively in CTR and TR using LEfSe. Conclusions - Overall, the use of Nisin A-based teat dip formulations was associated with a greater microbial diversity throughout the experimental period compared to commercial one. Although seasonality may have influenced the changes in the most prevalent taxa abundances, the experimental formulations maintained higher milk biodiversity, thus suggesting the ability of Nisin A to prevent alterations of milk microbiota.
ATKB-PID: An adaptive control method for micro tension under complex hot rolling conditions
Authors: Xinkai XU , Xiangrong SONG , Zheng QI
Published: July 2024

At present, the controller parameters in hot rolling roughing microtension control cannot be adaptively adjusted according to the changes of working conditions, which affects the width accuracy as well as the stability of production. Therefore, an ATKB-PID adaptive micro tension control method is proposed in this paper. A linear attention layer is added to the BP-PID controller and a KNN method is established to predict the optimal learning rate and inertia coefficient under actual working conditions. In addition, the objective function is designed according to the production index to improve the performance of the model. Through the comparison experiments with the widely used RBF-BP control and BP-PID control, it is verified that the ATKB-PID control method proposed in this paper has small steady state error and short adjustment time. The ATKB-PID control method can meet the complex and variable micro tension control conditions in thermal roughing, and has good application prospects.
Cross-disciplinary Analysis of Modern Chinese Classroom Interaction Discourse Patterns - An Empirical Study Based on Experienced Chinese Teacher
Authors: Yang Xiaoyu , Jansem Anchalee
Published: July 2024

This study adopts a cross-disciplinary perspective to focus on the interactive discourse in Modern Chinese classrooms, exploring the interactive pattern between teachers and students, discourse characteristics, and their impact on learning outcomes. Employing both classroom observation and conversation analysis, this empirical study was conducted on Modern Chinese classes in the Chinese Language and Literature Department of Z University. After a detailed analysis of the transcribed classroom interaction discourse, six main interaction discourse patterns were identified: IRF, [InRn]F, InRn, I[RnFn], [InRn]FR, and IR[FnRn]. The findings indicate that, while the traditional IRF model persists as the predominant form of classroom interaction, more complex interactive patterns (such as [InRn]F) are more effective in enhancing student participation and discourse volume in the classroom. This study offers practical recommendations on motivating student participation and elevating the quality of classroom interaction, providing valuable insights for the reform of Modern Chinese language teaching.
CRISPR/Cas9 alter NFYA binding site on CD44-regulating cis-element and control CD44 expression in breast cancer cells
Authors: Salem Sohair , Yahya Shaymaa MM
Published: July 2024

Background
CRISPR/Cas9 is used for editing of non-coding sequence. This study tested the involvement of downstream cis-element in regulating CD44 expression and the possibility of using CRISPR/Cas9 system to manipulate the transcription factor binding site. Bioinformatic tools predicted two binding sites (P1 and P2) for NFYA transcription factors. CRISPR/Cas9 was used to knockout NFYA gene and alter the NFYA binding site (P2).
Results
The data revealed decrease of CD44 gene expression and CD44+ CD24− sub-population after editing of P2 sequence more than the decrease resulted from editing of NFYA gene itself, confirming the involvement of NFYA in regulating CD44 gene. Both editing inhibited the migration ability of MDA-MB-231 cells. Unlike editing of NFYA gene, altering P2 sequence induced apoptosis. CHIP assay revealed that NFY have the ability to bind both P1 and P2 sequences, with higher enrichment in case of P2 than P1. After performing site-directed mutagenesis and luciferase assay we confirmed the involvement of both P1 and P2 in gene regulation, with higher potential in case of P2 than P1.
Conclusion
The findings confirmed the regulation of CD44 by NFYA and the efficacy of using CRISPR/Cas9 in altering the binding site, and downregulation of CD44.
Atmospheric NO2 Concentration Prediction with Statistical and Hybrid Deep Learning Methods
Authors: Uluocak Ihsan , Pinar Engin , Bilgili Mehmet
Published: July 2024

Recently, air pollution has become a critical environmental problem in Türkiye as well as in the world. Therefore, governments and scientists are putting a lot of effort into controlling air pollution and reducing its effects on human society. Scientists propose various models and methods for air quality forecasting because accurate estimation of air quality can provide basic decision-making support. This study proposes innovative hybrid models that integrate a Convolutional Neural Network (CNN) with a Long Short-Term Memory (LSTM) neural network and a Gated Recurrent Unit (GRU) to predict one day ahead of NO2 concentration. For this aim, the Time-Series Daily NO2 concentration data obtained between 2015 and 2022 at the Istanbul and Ankara provinces in Türkiye are used. The hybrid CNN-LSTM and CNN-GRU models are compared with various traditional statistical and machine-learning methods such as Autoregressive Moving Average (ARMA), Artificial Neural Network (ANN), CNN, LSTM, GRU, and Adaptive Neuro-Fuzzy Inference System (ANFIS-FCM). The accuracy of the prediction models is assessed using various statistical criteria and visual comparisons. Results show that the proposed hybrid CNN-LSTM and CNN-GRU models in one-day-ahead NO2 concentration predictions yield the best results among all models with R2 accuracy of 0.9547.
WITHDRAWN: Efficient Real-Time Quantile Regression for Streaming Data
Authors: Li Chris Junchi
Published: July 2024

Quantile regression (QR) is a powerful tool for modeling the conditional quantiles of a response variable given a set of covariates, offering a comprehensive portrayal of their relationship without assuming a specific distribution for the error term. This makes QR particularly suitable for analyzing skewed or heterogeneous data, with wide-ranging applications in fields such as economics and finance. However, traditional QR methods are often impractical for stream data, where data blocks arrive sequentially and demand real-time updates. To address this, we propose a Sequential Quantile Regression (SQR) algorithm that significantly enhances computational efficiency by converting the check loss optimization problem into a least squares problem. The SQR algorithm leverages a Bayesian framework for sequential updates, using the posterior distribution from previous data as the prior for new data. This approach allows for rapid, efficient updates suitable for real-time processing of stream data. We extend the SQR algorithm to Composite Quantile Regression (CQR) and provide theoretical analysis demonstrating the unbiasedness, asymptotic normality, and linear convergence of the SQR estimator. Simulation studies and real data analysis show that SQR offers favorable estimation and inferential performance while being significantly faster than existing methods. Our results highlight the potential of SQR for various applications requiring real-time data processing.
Commercialization of innovations in ukrainian book publishing: current trends and prospects
Authors: Khramova Karyna , Shymoshenko Anastasiia , Reshetniak Yaroslav
Published: July 2024

The article examines the peculiarities of innovative activity and commercialization of innovations in Ukrainian book publishing. Innovations are an essential factor in the development of the industry, as they make it possible to improve the quality of book products, make them more accessible and exciting for readers, and contribute to the popularization of reading. Because a third of Ukrainians do not read at all, the need to attract an audience that is used to an "easy" perception of information through watching videos, etc., is essential to developing a healthy and educated society. The hypothesis is that innovation activity in Ukrainian book publishing is not active enough, its development is relatively slow, and the implemented innovative projects are limited in number but of high quality. It is due to the conservatism of Ukrainian publishers, who are unwilling to enforce innovations if they involve too many additional risks, as well as the adverse effects of the COVID-19 pandemic and armed aggression against Ukraine. To test the hypothesis, this study analyzed the state of innovation in Ukrainian book publishing, examined the experience of Ukrainian publishers in implementing innovative projects, and identified trends in the development of innovation in Ukrainian book publishing. The analysis showed that innovation in Ukrainian book publishing has several trends and prospects. In particular, the popularity of e-books and audiobooks is growing, digital publishing is developing, new book formats are being introduced, and new technologies are being used to promote books both in the physical and digital space. Considering the current trends and prospects of innovative activity and commercialization of innovations in the Ukrainian book market, the industry needs a more comprehensive approach and the attraction of additional resources, both from the public and private sectors. In addition, to increase the number of readers and, accordingly, the development of the industry in Ukraine through the introduction of innovative technologies, we suggest moving to increasing the number of publications in electronic and audio formats, conducting experiments with the visual design of books, using environmentally friendly raw materials in the production process, developing digital publications in the format book sites for publishing poetry or short fiction, using QR codes, augmented reality and mobile applications with more paper editions, etc.
Национальные клинические рекомендации по диагностике и лечению Ph-негативных миелопролиферативных новообразований (истинной полицитемии, эссенциальной тромбоцитемии, первичного миелофиброза) (редакция 2024 г.)
Authors: Меликян Анаит Левоновна , Суборцева И. Н. , Ковригина А. М. , Шуваев В. А. , Морозова Е. В. , Ломаиа Е. Г. , Байков В. В. , Виноградова О. Ю. , Грицаев С. В. , Ионова Т. И. , Кузнецова П. И. , Кузьмина Л. А. , Мартынкевич И. С. , Митина Т. А. , Полушкина Е. С. , Поспелова Т. И. , Соколова М. А. , Судариков А. Б. , Танашян М. М. , Туркина А. Г. , Шатохин Ю. В. , Шмаков Р. Г. , Паровичникова Е. Н.
Published: July 2024

The development of National clinical guidelines on diagnosis and treatment of Ph-negative myeloproliferative neoplasms provides the knowledge basis for decision making in diagnosis, therapy, prevention, and rehabilitation in compliance with evidence-based medicine principles in order to ensure appropriate medical care in a particular clinical setting. The clinical guidelines underly the choice of optimal methods of diagnosis, therapy, prevention, and rehabilitation as part of a patient’s medical care, the development of criteria for the assessment of medical care quality, medical care standards, medical care procedures, setting standards on equipment and management of diagnostic and therapeutic procedures, and provision of continuous medical education and advanced training for healthcare professionals. In 2013, a working group was formed to develop and formulate clinical guidelines on the treatment of myeloproliferative neoplasms. These guidelines were first published in 2014, afterwards they were revised and republished. The dynamic development of current hematology presupposes constant updating of knowledge and implementation of new diagnosis and treatment methods in clinical practice. In this context, clinical guidelines present a dynamic document to be continuously amended, expanded, and updated in accordance with scientific findings and new requirements of specialists who are directly involved in treatment activities. The present edition is an upgraded version of clinical guidelines with updated information on classification, diagnosis, prognosis, current methods of therapy, and their efficacy and tolerability assessment. The guidelines are intended for oncologists, hematologists, healthcare executives, and medical students.
Radioprotective ingredients of a composite dairy product
Authors: Donskaja G. A. , Krekker L. G. , Kolosova E. V. , Bychkova T. S. , Karapetyan V. K.
Published: July 2024

Food products that have an anti-radiation effect and can be used in conditions of increased radiation are of particular interest. Scientifically based rationing of such products in the daily diet will make it possible to effectively use them to adapt the body to increased radiation and eliminate the effects of radiation exposure during treatment or examination. The purpose of the research presented in the paper is to analyze the nutritional and physiological causes of chronic nutrient deficiency of radioprotective microelements in the diet, to study the qualitative and biological characteristics of radioprotective ingredients to create a milk-based anti-radiation composition. The experimental part of the study has related to researching the mineral composition of selected ingredients and their antioxidant activity. The data obtained indicate that milk thistle flour contains a large amount of calcium, and sunflower meal contains potassium. In terms of potassium content, sunflower meal exceeds the microelement content in milk thistle by 50 %. The amount of calcium in milk thistle is 67 % higher compared to sunflower meal. In addition, plant raw materials are a source of magnesium, iron, zinc, copper, phosphorus and have pronounced antioxidant activity. As a result a scientific substantiation of the addition of ingredients for the production of radioprotective fermented milk product and its use in therapeutic and prophylactic nutrition for adults has been carried out in order to prevent radiation complications and remove radionuclides from the body. The normalized amount of ingredients for consumption per day has been calculated according to recommended diets, nutritional, physiological value, and physiological activity. Standards have been established for enriching micronutrients in the production of a radioprotective dairy product designed to increase the body's resistance to the action of free radical processes and enhance antioxidant capacity under conditions of radiation exposure.
Education of environmental responsibility of students based on integral-dialogue technology
Authors: Loshchilova A. A. , Vinokurova N. F.
Published: July 2024

The article represents the authors’ approach to the formation of environmental responsibility of students based on integral dialogue technology meeting the general trend to humanization of educational process and the raising importance of environmental awareness. The article reveals the integral potential of cultural heritage, materializing the ideas of cocreation between man and nature, and national values and traditions of the peoples of the country. The authors define the theoretical and methodological basis for the development of an integraldialogue technology for educating environmental responsibility, and reveal the substantive aspect of technology that is associated with the use of historical and cultural heritage. The essence and procedural features of integral-dialogue technology, which provides for the consistent implementation of cultural-ecological situations-immersions in the historical and cultural heritage, are considered. The sequence of such situations is built taking into account the stages of formation of environmental responsibility and includes three stages: (1) focusing on the whole: meeting; (2) focusing on the part: dialogue; (3) focusing on the whole: acting responsibly1. These stages provide a systematic consideration of the historical and cultural heritage through the categorical matrix of the whole and the part, as well as a holistic impact on all spheres of the individual’s consciousness in the process of educating environmental responsibility.
FIRSATÇI DAVRANIŞLARA KONU OLAN UYARLAMA KAYITLARININ GEÇERLİLİĞİ
Authors: Şahin Hale
Published: July 2024

Taraflar, sözleşmenin içeriğini sözleşmeyle elde etmek istedikleri menfaatleri dikkate alarak belirlemektedir. Fakat, tarafların sözleşmenin içeriğine ilişkin bütün koşulları sözleşmenin müzakeresi sırasında göz önünde bulundurmaları hukuk düzeni tarafından onlardan beklenmemektedir. Çünkü sözleşmenin müzakeresi esnasında, sözleşmenin kurulmasından sonra ortaya çıkacak durum değişikliklerinin taraflarca öngörülmesi çoğunlukla mümkün değildir. Sözleşme özgürlüğü ilkesi gereği taraflar hem öngörülebilir hem de öngörülemeyen durum değişikliklerinin borç ilişkisine etkisini sözleşmede düzenleyebilir. Sözleşmenin kurulması sırasında sağlanan menfaatler dengesinin gelecekteki durum değişiklikleri sonucu bozulmasını ve sözleşmenin uyarlanması ihtiyacını doğurmasını önlemek amacıyla tarafların sözleşmede kararlaştırdıkları sözleşme hükümleri uyarlama kayıtları olarak adlandırılmaktadır. Bu tür kayıtlar, sözleşme özgürlüğünün sınırlarına uygun olarak düzenlenmelidir. Bununla birlikte, uyarlama kayıtları sözleşme özgürlüğünün sınırlarına uygun olarak kaleme alınsa dahi taraflardan biri tarafından karşı taraf aleyhine fırsatçı davranış oluşturacak şekilde kullanılabilir. Uyarlama kayıtlarının fırsatçı davranışlara konu olduğu hâllerde, hâkim tarafından sözleşmeye müdahale edilmesi gerekir. Çalışmamızda öncelikle uyarlama kayıtları ve fırsatçı davranış kavramları açıklanmıştır. Daha sonra, uyarlama kayıtlarının fırsatçı davranış oluşturacak şekilde kullanımına yönelik çeşitli örnekler verilmiş ve bu tür kayıtların hukuki geçerliliği üzerinde durulmuştur.
Condições de saúde de idosos com restrição de mobilidade assistidos pela atenção primária
Authors: Henicka Rúbia de Oliveira , Winkelmann Eliane Roseli , Berlezi Evelise Moraes
Published: July 2024

Com o envelhecimento populacional o perfil de morbimortalidade da população brasileira tem enfrentado mudanças importantes e preocupantes; especialmente porque as doenças mais prevalentes são doenças com potencial para gerar incapacidades e consequentemente redução de mobilidade. Este cenário epidemiológico instiga o olhar crítico sobre o sistema de saúde brasileiro. O objetivo do estudo é produzir um diagnóstico das condições de vida e saúde de idosos com restrição de mobilidade assistidos pela Atenção Primária à Saúde. Estudo transversal realizado de agosto de 2021 a dezembro de 2022 no município de Ijuí/RS/Brasil com idosos que estão acamados em seu domicílio ou com restrição de mobilidade por meio de uma entrevista no domicílio sobre condições gerais de vida e saúde. Amostra de 195 idosos; 65,6% eram mulheres, 53,3% tinham 80 anos ou mais; 21% estavam em cadeiras de rodas, 55,9% eram domiciliados e 21,3% restrito ao leito; mais de um terço dos idosos apresentam algum grau de dependência para a realização das atividades cotidianas; 74,4% dos idosos necessitam de cuidador; 95,4% dos idosos fazem uso de medicação de forma contínua. Conclui-se que o diagnóstico das condições de saúde de idosos com restrição de mobilidade assistidos pela Atenção Primária à Saúde mostram situação de dependência que requerem a promoção de políticas públicas.
Enhanced fluoride removal from water using acid-modified red clay soil from the Loess Plateau of China
Authors: Zhu Bingzi , Duan Runbin , Li Yuzhen , Lu Xinyu , Sun Yao , Gao Jiangqi
Published: July 2024

The discharge of fluoride-containing wastewater poses a severe threat to global water resources, ecosystems, and human health. Urgently needed are economically feasible and environmentally sustainable solutions for worldwide fluoride contamination. This study explores utilizing unmodified and modified red clay soils from China's Loess Plateau as adsorbents for fluoride mitigation. Sulfuric acid-modified red clay soil showed higher fluoride removal than unmodified, NaOH-modified, and thermally modified soils. Fluoride adsorption decreased with rising pH from 2.0 to 10.0 for unmodified (67.67–3.91%) and acid-modified red clay soil (90.44–32.06%). The Langmuir model better described the data (R2 = 0.9821, 0.9901 for unmodified, acid-modified soil), improving maximum adsorption capacity by 252%. Pseudo-second-order kinetics (R2 = 0.9925, 0.9954 for unmodified, acid-modified soil) accurately described the kinetic data. Acid modification improved reaction rates, shortening the breakpoint from 6.694 to 2.318 min1/2. Over time, the process transitioned from intraparticle diffusion to external mass transfer and intraparticle diffusion. FTIR analysis showed that acid modification strengthened ligand exchange and provided ion exchange opportunities. This study advances fluoride adsorption through innovative clay soil utilization, offering economical, viable, and environmentally friendly solutions.