bluuebunny/crossref_metadata_2025 huggingface dataset:
Fur Cortisol in French Bulldogs with Different Manifestations of Brachycephalic Obstructive Airway Syndrome
Authors: Schroers Maike , Goossens Juliette , Zablotski Yury , Meyer-Lindenberg Andrea
Published: March 2024

In the present study, 33 French bulldogs with varying degrees of brachycephalic obstructive airway syndrome (BOAS) were evaluated for various parameters to provide evidence of chronic stress in the dogs. For this purpose, an owner interview, a clinical examination, and cortisol concentrations in the fur of the dogs were collected. The median cortisol concentration in the fur of the dogs suffering from BOAS (n = 19) was 0.99 pg/mg (range 0.63–66.18), while that of the control group (n = 14) was 1.13 pg/mg (range 0.3–43.45). However, the statistical analysis did not reveal a statistically significant difference; therefore, it is not possible to distinguish between dogs clinically affected with BOAS and those without clinically relevant signs of the disease. There was no statistically significant difference between the age of the animals and the fur cortisol. None of the owners in the examination and control groups indicated that their dog was suffering from chronic stress which shows that the disease is easily underestimated and remains common. Thus, a thorough clinical examination by an experienced veterinarian remains the gold standard in order to diagnose BOAS.
Response to Incremental Replacement of Palm Oil with Fish Oil in Starter Diet on Growth Performance, Plasma Metabolites, Ruminal Fermentation, and Behavior of Dairy Calves
Authors: Hosseini Seyed Hadi , Mirzaei-Alamouti Hamidreza , Mansouryar Morteza , Vazirigohar Mina , Rezamand Pedram , Mahjoubi Ehsan , Ramezankhani Jafar , Aschenbach Jörg R.
Published: March 2024

The objective of this study was to evaluate the effects of the incremental levels of n-3 fatty acids (FA) in starter feed (SF) on growth and metabolic performance of milk-fed calves. From day 3 of age, 30 female calves (39.4 ± 3.1 kg of body weight) were randomly assigned to one of three dietary treatments: (1) SF supplemented with 3.3% palm fatty acids (PO), (2) SF supplemented with 1.7% of PO and 1.9% fish oil (PFO), or (3) SF supplemented with 3.9% fish oil (FO). Chopped straw (7.5% of DM) was included in the SF of all treatments as total mixed ration (TMR). Diets had similar energy and protein contents. Total n-3 FA (% of total FA) and n-6/n-3 of PO, PFO, and FO were 1.90, 6.80, and 11.8 and 15.5, 4.50, and 2.70, respectively. The BW was greater for calves receiving FO (60.2 ± 0.3 kg) compared with PFO (58.7 ± 0.3 kg; p = 0.007) and tended to be greater for calves receiving FO vs. PO (59.0 ± 0.3 kg; p = 0.050). Because there was no interaction effect between diet × week of experiment, the greater BW of FO could not be attributed to the dietary treatment. Accordingly, average daily gain, total dry matter intake (DMI), starter DMI, and gain to intake ratio (G:FI) did not differ among dietary treatments during the entire period of the study (p > 0.05). Dietary treatments did not impact body size parameters such as body length, body girth, withers height, heart girth, hip height, and width (p > 0.05). Neither ruminal fermentation parameters nor blood variables were influenced by supplementing the types of oil at different time points. Calves’ behavioral parameters, such as standing, lying, eating, and ruminating, were not influenced by different dietary treatments (p > 0.05). The number of days with abnormal fecal score was not different among dietary groups (p > 0.05). Overall, our findings suggest that changing the n-6/n-3 ratio in starter feed by incremental replacement of palm fatty acid with fish oil at a moderate supplemental level of ~3% of DM may not affect the growth and metabolic performance of young calves under non-challenged conditions.
Characterization of Normal Bone in the Equine Distal Limb with Effective Atomic Number and Electron Density Determined with Single-Source Dual Energy and Detector-Based Spectral Computed Tomography
Authors: Steiner Janine , Richter Henning , Kaufmann Rolf , Ohlerth Stefanie
Published: March 2024

Single-source dual energy (SSDECT) and detector-based spectral computed tomography (DBSCT) are emerging technologies allowing the interrogation of materials that have different attenuation properties at different energies. Both technologies enable the calculation of effective atomic number (EAN), an index to determine tissue composition, and electron density (ED), which is assumed to be associated with cellularity in tissues. In the present prospective observational study, EAN and ED values were determined for 16 zones in normal subchondral and trabecular bone of 37 equine cadaver limbs. Using both technologies, the following findings were obtained: 1. palmar/plantar EAN zone values in the fetlock increased significantly with increasing age of the horse; 2. all EAN and ED values were significantly lower in the trabecular bone than in the subchondral bone of all phalanges; 3. in the distal phalanx and navicular bone, most EAN and ED values were significantly lower compared to the proximal and middle phalanx; and 4. some EAN and ED values were significantly different between front and hind limbs. Several EAN and ED values significantly differed between SSDECT and DBSCT. The reported EAN and ED values in the subchondral and trabecular bone of the equine distal limb may serve as preliminary reference values and aid future evaluation and classification of diseases.
Anatomy and Physiology of Water Buffalo Mammary Glands: An Anatomofunctional Comparison with Dairy Cattle
Authors: Mota-Rojas Daniel , Napolitano Fabio , Chay-Canul Alfonso , Ghezzi Marcelo , Braghieri Ada , Domínguez-Oliva Adriana , Bragaglio Andrea , Álvarez-Macías Adolfo , Olmos-Hernández Adriana , De Rosa Giuseppe , García-Herrera Ricardo , Lendez Pamela , Pacelli Corrado , Bertoni Aldo , Barile Vittoria Lucia
Published: March 2024

The present review aims to analyze the anatomical and physiological characteristics of the mammary gland and udders of water buffalo by making an anatomofunctional comparison with dairy cattle. It will also discuss the knowledge generated around the physiological regulation of milk ejection in the water buffalo. It was found that buffalo’s average udder depth and width is approximately 20 cm smaller than Bos cattle. One of the main differences with dairy cattle is a longer teat canal length (around 8.25–11.56 cm), which highly influences buffalo milking. In this sense, a narrower teat canal (2.71 ± 0.10 cm) and thicker sphincter muscle are associated with needing higher vacuum levels when using machine milking in buffalo. Moreover, the predominant alveolar fraction of water buffalo storing 90–95% of the entire milk production is another element that can be related to the lower milk yields in buffalo (when compared to Bos cattle) and the requirements for prolonged prestimulation in this species. Considering the anatomical characteristics of water buffalo’s udder could help improve bubaline dairy systems.
Effects of Garlic Oil and Cinnamaldehyde on Sheep Rumen Fermentation and Microbial Populations in Rusitec Fermenters in Two Different Sampling Periods
Authors: García-Rodríguez Jairo , Saro Cristina , Mateos Iván , Carro María Dolores , Ranilla María José
Published: March 2024

Garlic oil (GO) and cinnamaldehyde (CIN) have shown potential to modify rumen fermentation. The aim of this study was to assess the effects of GO and CIN on rumen fermentation, microbial protein synthesis (MPS), and microbial populations in Rusitec fermenters fed a mixed diet (50:50 forage/concentrate), as well as whether these effects were maintained over time. Six fermenters were used in two 15-day incubation runs. Within each run, two fermenters received no additive, 180 mg/L of GO, or 180 mg/L of CIN. Rumen fermentation parameters were assessed in two periods (P1 and P2), and microbial populations were studied after each of these periods. Garlic oil reduced the acetate/propionate ratio and methane production (p < 0.001) in P1 and P2 and decreased protozoal DNA concentration and the relative abundance of fungi and archaea after P1 (p < 0.05). Cinnamaldehyde increased bacterial diversity (p < 0.01) and modified the structure of bacterial communities after P1, decreased bacterial DNA concentration after P2 (p < 0.05), and increased MPS (p < 0.001). The results of this study indicate that 180 mg/L of GO and CIN promoted a more efficient rumen fermentation and increased the protein supply to the animal, respectively, although an apparent adaptive response of microbial populations to GO was observed.
Canine Upper Digestive Tract 3D Model: Assessing Its Utility for Anatomy and Upper Endoscopy Learning
Authors: Díaz-Regañón David , Mendaza-De Cal Rosa , García-Sancho Mercedes , Rodríguez-Franco Fernando , Sainz Ángel , Rodriguez-Quiros Jesus , Rojo Concepción
Published: March 2024

A teaching strategy using 3D-printed models of the canine upper digestive tract (UDT) for anatomy demonstration and upper endoscopy instruction was evaluated. The canine UDT (esophagus–stomach–duodenum) was scanned and 3D-printed molds were manufactured using silicone casting. First-year students were introduced to these 3D models in practical sessions alongside real specimens. Simultaneously, fifth-year students were trained in endoscope handling and anatomical recognition using 3D specimens. Both groups completed an anonymous survey. Results showed that overall, first-year (n = 93) and fifth-year (n = 45) students agreed or strongly agreed that the 3D-printed model was effective for learning purposes. In summary, first-year students highlighted an improved understanding of size, volume, topography, and easier manipulation of the 3D model compared to fresh specimens. Fifth-year students were more enthusiastic, finding the 3D model valuable for spatial vision and clinical training. While both groups were against completely replacing the natural UDT with the 3D model, first-year students were more hesitant. These findings suggest that the 3D model of the canine UDT is an effective tool for hands-on training in clinical endoscopy and a valuable, albeit complementary, resource for teaching anatomy and topography.
Anti-Staphylococcal Biofilm Effects of a Liposome-Based Formulation Containing Citrus Polyphenols
Authors: Mazzantini Diletta , Massimino Mariacristina , Calvigioni Marco , Rossi Virginia , Celandroni Francesco , Lupetti Antonella , Batoni Giovanna , Ghelardi Emilia
Published: March 2024

Biofilms are surface-associated microbial communities embedded in a matrix that is almost impenetrable to antibiotics, thus constituting a critical health threat. Biofilm formation on the cornea or ocular devices can lead to serious and difficult-to-treat infections. Nowadays, natural molecules with antimicrobial activity and liposome-based delivery systems are proposed as anti-biofilm candidates. In this study, the anti-biofilm activity of a formulation containing citrus polyphenols encapsulated in liposomes was evaluated against Staphylococcus aureus and Staphylococcus epidermidis, the most common agents in ocular infections. The formulation activity against planktonic staphylococci was tested by broth microdilution and sub-inhibitory concentrations were used to evaluate the effect on biofilm formation using the crystal violet (CV) assay. The eradicating effect of the preparation on mature biofilms was investigated by the CV assay, plate count, and confocal laser scanning microscopy. The product was bactericidal against staphylococci at a dilution of 1:2 or 1:4 and able to reduce biofilm formation even if diluted at 1:64. The formulation also had the ability to reduce the biomass of mature biofilms without affecting the number of cells, suggesting activity on the extracellular matrix. Overall, our results support the application of the used liposome-encapsulated polyphenols as an anti-biofilm strategy to counter biofilm-associated ocular infections.
Epidemiology, Clinical, and Microbiological Characteristics of Multidrug-Resistant Gram-Negative Bacteremia in Qatar
Authors: Abdel Hadi Hamad , Dargham Soha R. , Eltayeb Faiha , Ali Mohamed O. K. , Suliman Jinan , Ahmed Shiema Abdalla M. , Omrani Ali S. , Ibrahim Emad Bashir , Chen Yuzhou , Tsui Clement K. M. , Skariah Sini , Sultan Ali
Published: March 2024

Antimicrobial resistance is a global healthcare threat with significant clinical and economic consequences peaking at secondary and tertiary care hospitals where multidrug-resistant Gram-negative bacteria (MDR GNB) lead to poor outcomes. A prospective study was conducted between January and December 2019 for all invasive bloodstream infections (BSIs) secondary to MDR GNB in Qatar identified during routine microbiological service to examine their clinical, microbiological, and genomic characteristics. Out of 3238 episodes of GNB BSIs, the prevalence of MDR GNB was 13% (429/3238). The predominant MDR pathogens were Escherichia coli (62.7%), Klebsiella pneumoniae (20.4%), Salmonella species (6.6%), and Pseudomonas aeruginosa (5.3%), while out of 245 clinically evaluated patients, the majority were adult males, with the elderly constituting almost one-third of the cohort and with highest observed risk for prolonged hospital stays. The risk factors identified included multiple comorbidities, recent healthcare contact, previous antimicrobial therapy, and admission to critical care. The in-hospital mortality rate was recorded at 25.7%, associated with multiple comorbidities, admission to critical care, and the acquisition of MDR Pseudomonas aeruginosa. Resistant pathogens demonstrated high levels of antimicrobial resistance but noticeable susceptibility to amikacin and carbapenems. Genomic analysis revealed that Escherichia coli ST131 and Salmonella enterica ST1 were the predominant clones not observed with other pathogens.
7-O-tyrosyl Silybin Derivatives as a Novel Set of Anti-Prostate Cancer Compounds
Authors: Romanucci Valeria , Pagano Rita , Kandhari Kushal , Zarrelli Armando , Petrone Maria , Agarwal Chapla , Agarwal Rajesh , Di Fabio Giovanni
Published: March 2024

Silybin is a natural compound extensively studied for its hepatoprotective, neuroprotective and anticancer properties. Envisioning the enhancement of silybin potential by suitable modifications in its chemical structure, here, a series of new 7-O-alkyl silybins derivatives were synthesized by the Mitsunobu reaction starting from the silybins and tyrosol-based phenols, such as tyrosol (TYR, 3), 3-methoxytyrosol (MTYR, 4), and 3-hydroxytyrosol (HTYR, 5). This research sought to explore the antioxidant and anticancer properties of eighteen new derivatives and their mechanisms. In particular, the antioxidant properties of new derivatives outlined by the DPPH assay showed a very pronounced activity depending on the tyrosyl moiety (HTYR > MTYR >> TYR). A significant contribution of the HTYR moiety was observed for silybins and 2,3-dehydro-silybin-based derivatives. According to the very potent antioxidant activity, 2,3-dehydro-silybin derivatives 15ab, 15a, and 15b exerted the most potent anticancer activity in human prostate cancer PC-3 cells. Furthermore, flow cytometric analysis for cell cycle and apoptosis revealed that 15ab, 15a, and 15b induce strong G1 phase arrest and increase late apoptotic population in PC-3 cells. Additionally, Western blotting for apoptotic marker cleaved caspase-3 confirmed apoptosis induction by these silybin derivatives in PC-3 cells. These findings hold significant importance in the investigation of anticancer properties of silybin derivatives and strongly encourage swift investigation in pre-clinical models and clinical trials.
Loranthus tanakae Franch. and Sav. Attenuates Respiratory Inflammation Caused by Asian Sand Dust
Authors: Lee Se-Jin , Pak So-Won , Lee A Yeong , Kim Woong-Il , Chae Sung-Wook , Cho Young-Kwon , Ko Je-Won , Kim Tae-Won , Kim Jong-Choon , Moon Byeong Cheol , Seo Yun-Soo , Shin In-Sik
Published: March 2024

Asian sand dust (ASD), generally produced in East Asia, including China, Japan, and Korea, directly leads to the development of pulmonary disease and exacerbates underlying pulmonary diseases. Loranthus tanakae Franch. and Sav. is a traditional herbal medicine applied to improve various inflammatory conditions. Here, we evaluated the curative properties of L. tanakae ethanol extract (LTE) against pulmonary inflammation caused by ASD. Additionally, to investigate the mechanism of action of LTE, we performed network pharmacological analysis. ASD was administrated on day 1, 3, and 5 by intranasal instillation, and LTE was orally administered for 6 days. Administration of LTE significantly decreased inflammatory cytokines and the number of inflammatory cells in bronchoalveolar lavage fluid, which was accompanied by a decrease in inflammatory cell accumulation in pulmonary tissue. Administration of LTE decreased the expression of cyclooxygenase2 and matrix metalloproteinase-9 in mice exposed to ASD with the decline in p65 phosphorylation. Additionally, administration of LTE significantly elevated hemeoxygenase (HO)-1 expression in the pulmonary tissue of mice exposed to ASD. These results were consistent with the data of network pharmacological analysis. This experiment showed that LTE attenuated pulmonary inflammation caused by ASD via inhibition of NF-κB and elevation of HO-1. Therefore, LTE may have potential as a therapeutic agent to treat pulmonary inflammation caused by ASD.
The Effects of Dietary n-3 Highly Unsaturated Fatty Acids on Growth, Antioxidant Capacity, Immunity, and Oxylipin Profiles in Acipenser dabryanus
Authors: Wu Jinping , Xiong Wei , Liu Wei , Wu Jinming , Ruan Rui , Fu Peng , Wang Yuqi , Liu Yuan , Leng Xiaoqian , Li Pengcheng , Zhong Jia , Zhang Chuang , Du Hao
Published: March 2024

Currently, the effects of dietary levels of n-3 highly unsaturated fatty acids (HUFAs) on the growth performance, antioxidant capacity, immunity, and serum oxylipin profiles of female F2-generation Yangtze sturgeon remain unknown. A total of 75 Yangtze sturgeons, an endangered freshwater fish species, with an average body weight of 3.60 ± 0.83 kg, were randomly allocated to 15 concrete pools, with each dietary group represented by 5 fish per pool. The fish were fed five different experimental diets containing various levels of n-3 HUFAs (0.5%, 1.0%, 1.5%, 2.0%, and 2.4%). After a feeding period of 5 months, no significant differences in the growth performances of the fish were observed among the five dietary groups (p > 0.05). However, we did note that the serum levels of low-density lipoprotein cholesterol (LDL-C), triglycerides (TGs), and total cholesterol (TCHO) exhibited a marked increase in the fish that consumed higher dietary n-3 HUFA levels (p < 0.05). Conversely, alkaline phosphatase (ALP) activities showed a notable decrease as dietary n-3 HUFA levels increased (p < 0.05). Serum antioxidant indices, such as the activity levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), were significantly higher in the 2.4% HUFA group compared to the 0.5% HUFA group. Additionally, muscle antioxidant indices, including total antioxidant capacity (T-AOC), catalase (CAT), and SOD activity, exhibited notable increases as dietary n-3 HUFA levels increased (p < 0.05). Furthermore, there was a decrease in malondialdehyde (MDA) levels as dietary n-3 HUFA levels increased (p < 0.05). In relation to immune indices, only serum immunoglobulin M (IgM) and muscle complement 3 (C3) were found to be influenced by dietary n-3 HUFA levels (p < 0.05). A total of 80 oxylipins were quantified, and our subsequent K-means cluster analysis resulted in the classification of 62 oxylipins into 10 subclasses. Among the different n-3 HUFA diets, a total of 14 differential oxylipins were identified in the sera. These findings demonstrate that dietary supplementation with n-3 HUFAs exceeding a 1.0% level can enhance antioxidant capacity and regulate serum lipid metabolism, potentially through modulation of oxylipins derived from ARA, DHA, and EPA. These insights provide novel perspectives on the mechanisms underlying these observations.
Potential New Target for Dry Eye Disease—Oxidative Stress
Authors: Bu Jinghua , Liu Yanbo , Zhang Rongrong , Lin Sijie , Zhuang Jingbin , Sun Le , Zhang Lingyu , He Hui , Zong Rongrong , Wu Yang , Li Wei
Published: March 2024

Dry eye disease (DED) is a multifactorial condition affecting the ocular surface. It is characterized by loss of tear film homeostasis and accompanied by ocular symptoms that may potentially result in damage to the ocular surface and even vision loss. Unmodifiable risk factors for DED mainly include aging, hormonal changes, and lifestyle issues such as reduced sleep duration, increased screen exposure, smoking, and ethanol consumption. As its prevalence continues to rise, DED has garnered considerable attention, prompting the exploration of potential new therapeutic targets. Recent studies have found that when the production of ROS exceeds the capacity of the antioxidant defense system on the ocular surface, oxidative stress ensues, leading to cellular apoptosis and further oxidative damage. These events can exacerbate inflammation and cellular stress responses, further increasing ROS levels and promoting a vicious cycle of oxidative stress in DED. Therefore, given the central role of reactive oxygen species in the vicious cycle of inflammation in DED, strategies involving antioxidants have emerged as a novel approach for its treatment. This review aims to enhance our understanding of the intricate relationship between oxidative stress and DED, thereby providing directions to explore innovative therapeutic approaches for this complex ocular disorder.
Antioxidant Activity of Bilirubin in Micellar and Liposomal Systems Is pH-Dependent
Authors: Przybylski Paweł , Żebrowski Michał , Witkowski Wojciech , Cybularczyk-Cecotka Martyna , Litwinienko Grzegorz
Published: March 2024

Bilirubin (BR), a product of heme catabolism, plays a critical role in biological systems. Although increased levels of BR result in hyperbilirubinemia or jaundice, there is increasing evidence that lower concentrations substantially decrease the risk of oxidative stress-mediated diseases due to antioxidant functions of BR. We studied the radical-trapping ability of BR in two model systems, micellar and liposomal, at a broad pH range. At pH < 6.0, BR behaves as a retardant; however, at pH ≥ 6.0, BR becomes strong radical trapping antioxidant, with rate constants for reaction with lipidperoxyl radicals (kinh) within the range from 1.2 × 104 M−1 s−1 to 3.5 × 104 M−1 s−1, and in liposomal system, the activity of BR is comparable to α-tocopherol. This transition is likely facilitated by the ionization of carboxyl groups, leading to a conformational shift in BR and improved solubility/localization at the water/lipid interface. This is the first experimental evidence of the role of pH on the antioxidant activity of bilirubin, and the observed pH-dependent radical-trapping ability of BR holds practical significance, particularly in jaundice treatment where light therapy targets the skin’s weakly acidic surface. Minor adjustments toward neutral or alkaline pH can enhance radical-trapping action of BR, thereby mitigating oxidative stress induced with blue or violet light exposure.
Chitosan-Loaded Lagenaria siceraria and Thymus vulgaris Potentiate Antibacterial, Antioxidant, and Immunomodulatory Activities against Extensive Drug-Resistant Pseudomonas aeruginosa and Vancomycin-Resistant Staphylococcus aureus: In Vitro and In Vivo Approaches
Authors: Taha Selwan M. , Abd El-Aziz Norhan K. , Abdelkhalek Adel , Pet Ioan , Ahmadi Mirela , El-Nabtity Sameh M.
Published: March 2024

Antimicrobial resistance poses considerable issues for current clinical care, so the modified use of antimicrobial agents and public health initiatives, coupled with new antimicrobial approaches, may help to minimize the impact of multidrug-resistant (MDR) bacteria in the future. This study aimed to evaluate the antimicrobial, antioxidant, and immunomodulatory activities of Lagenaria siceraria, Thymus vulgaris, and their chitosan nanocomposites against extensive drug-resistant (XDR) Pseudomonas aeruginosa and vancomycin-resistant Staphylococcus aureus (VRSA) using both in vitro and in vivo assays. The in vitro antimicrobial susceptibilities of P. aeruginosa and VRSA strains revealed 100% sensitivity to imipenem (100%). All P. aeruginosa strains were resistant to cefoxitin, cefepime, trimethoprim + sulfamethoxazole, and fosfomycin. However, S. aureus strains showed a full resistance to cefoxitin, amoxicillin, ampicillin, erythromycin, chloramphenicol, and fosfomycin (100% each). Interestingly, all S. aureus strains were vancomycin-resistant (MIC = 32–512 μg/mL), and 90% of P. aeruginosa and S. aureus strains were XDR. The antimicrobial potential of Lagenaria siceraria and Thymus vulgaris nanocomposites with chitosan nanoparticles demonstrated marked inhibitory activities against XDR P. aeruginosa and VRSA strains with inhibition zones’ diameters up to 50 mm and MIC values ranging from 0.125 to 1 μg/mL and 1 to 8 μg/mL, respectively. The results of the in vivo approach in male Sprague Dawley rats revealed that infection with P. aeruginosa and S. aureus displayed significant changes in biochemical, hematological, and histopathological findings compared to the negative control group. These values returned to the normal range after treatment by chitosan nanoparticles, either loaded with Lagenaria siceraria or Thymus vulgaris. Real-time quantitative polymerase chain reaction (RT-qPCR) findings presented significant upregulation of the relative expression of the IL10 gene and downregulation of the IFNG gene throughout the experimental period, especially after treatment with chitosan nanoparticles loaded either with Lagenaria siceraria or Thymus vulgaris in comparison to the positive control groups. In conclusion, this is the first report suggesting the use of Lagenaria siceraria and Thymus vulgaris nanocomposites with chitosan nanoparticles as a promising contender for combating XDR P. aeruginosa and VRSA infections as well as a manager for inflammatory situations and oxidative stress-related disorders.
Numerical Simulation of Aerodynamic Pressure on Sound Barriers from High-Speed Trains with Different Nose Lengths
Authors: Jin Jie , Liu Dongyun , Tu Yongming
Published: March 2024

For high-speed railway sound barriers, determining the aerodynamic pressure generated by high-speed trains is crucial for their structural design. This paper investigates the distribution of aerodynamic pressure on the sound barrier caused by high-speed trains with different nose lengths, utilizing the computational fluid dynamics (CFD) simulation method. The accuracy of the numerical simulation method employed is verified through comparison with field test results from the literature. Research findings reveal that when a high-speed train passes through a sound barrier, significant “head wave” and “wake wave” effects occur, with the pressure peak of the “head wave” being notably greater than that of the “wake wave”. As the distance between the sound barrier and the center of the train gradually increases, the aerodynamic pressure on the sound barrier gradually decreases. The nose length of the train has a considerable impact on the aerodynamic pressure exerted on the sound barrier. The streamlined shape of longer-nose trains can significantly reduce the aerodynamic effects on the sound barrier, resulting in a notably smaller pressure peak compared to shorter-nose trains. Finally, by establishing the relationship between the train nose length and the aerodynamic pressure peak, a calculation formula for the train-induced aerodynamic pressure acting on the sound barrier is proposed, taking into account the nose length of the high-speed train.
Uncertainty-Guided Asymmetric Consistency Domain Adaptation for Histopathological Image Classification
Authors: Yu Chenglin , Pei Hailong
Published: March 2024

Deep learning has achieved remarkable progress in medical image analysis, but its effectiveness heavily relies on large-scale and well-annotated datasets. However, assembling a large-scale dataset of annotated histopathological images is challenging due to their unique characteristics, including various image sizes, multiple cancer types, and staining variations. Moreover, strict data privacy in medicine severely restricts data sharing and poses significant challenges in acquiring large-scale and well-annotated histopathological images. To tackle these constraints, Transfer Learning (TL) provides a promising solution by exploiting knowledge from another domain. This study proposes the Uncertainty-guided asymmetric Consistency Domain Adaptation (UCDA), which does not require accessing the source data and is composed of two essential components, e.g., Uncertainty-guided Source-free Transfer Learning (USTL) and Asymmetric Consistency Learning (ACL). In detail, USTL facilitates a secure mapping of the source domain model’s feature space onto the target domain, eliminating the dependency on source domain data to protect data privacy. At the same time, the ACL module measures the symmetry and asymmetry between the source and target domains, bridging the information gap and preserving inter-domain differences among medical images. We comprehensively evaluate the effectiveness of UCDA on three widely recognized and publicly available datasets, namely NCTCRC-HE-100K, PCam, and LC25000. Impressively, our proposed method achieves remarkable performance on accuracy and F1-scores. Additionally, feature visualizations effectively demonstrate the exceptional generalizability and discriminative power of the learned representations. These compelling results underscore the significant potential of UCDA in driving the advancement of deep learning techniques within the realm of histopathological image analysis.
Processing, Quality and Elemental Safety of Fish
Authors: Cortés-Sánchez Alejandro De Jesús , Diaz-Ramírez Mayra , Torres-Ochoa Erika , Espinosa-Chaurand Luis Daniel , Rayas-Amor Adolfo Armando , Cruz-Monterrosa Rosy G. , Aguilar-Toalá José Eleazar , Salgado-Cruz Ma. de la Paz
Published: March 2024

Fish is a food that is widely produced, marketed, and consumed around the world. It is a basic component of human nutrition due to its chemical and nutritional composition, but at the same time is highly perishable and susceptible to contamination throughout the food chain, compromising its quality and safety. Fishing and aquaculture products, being perishable, require adequate processing and preservation to maintain their quality and safety as much as possible until their acquisition and final consumption. Different preservation methods have been developed and used to extend the shelf life of fish products with minimal impact on their nutritional and organoleptic properties. Therefore, the objective of this document is to provide, through the search, analysis, and selection of information from various databases such as Scopus, Scielo, Web of Science, and Google Scholar, among others, a general and basic informative description of fish, aimed at academics, producers, industrialists, and the general public that allows them to identify the basic points in the processing and preservation of the quality and safety of fish. Aspects related to production; the nutritional qualities of fish; the various physical, chemical, and biological contaminating hazards of fish; the control and prevention of contaminants and diseases for consumers; and food legislation for products are included, as well as the main traditional and modern methods applied in the preservation of fishery and aquaculture products to maintain their nutritional value, quality, and safety that allow for the availability of food and the protection of public health.
In Vitro Study of the Surface Roughness, Hardness, and Absorption of an Injection-Molded Denture Base Polymer, Manufactured under Insufficient Mold Solidification
Authors: Chuchulska Bozhana , Dimitrova Mariya , Dochev Boyan
Published: March 2024

The current study sought to investigate the changes in surface hardness, roughness, and moisture absorption of the Vertex ThermoSens polymer (Vertex Dental, 3D Systems, The Netherlands) following immersion in artificial saliva for various periods (7, 14, and 28 days). A total of 60 rectangular specimens with dimensions of 20 mm in length, 20 mm in width, and 3 mm in thickness were made. Due to insufficient mold solidification, these specimens were made utilizing the injection molding process. A Mitutoyo Surftest 4 roughness meter (Mitutoyo, Aurora, IL, USA) was used to measure the surface roughness of the test materials. The ThermoSens polymer hardness was assessed using the Shor method and D—HSD scale, while absorption was measured with a Sartorius analytical balance. Results indicated the highest mean hardness after 28 days (M = 77.6) (Surface 1) and the lowest for the control group (M = 59) (Surface 2). The maximum surface roughness occurred in direction 2.2 pre-immersion (Ra = 2.88 μm) and 7 days post-removal (Ra = 2.95 μm). The control group exhibited the lowest absorption (Wsp = 1.524 mg/mm3), with the highest mean values over 28 days (Wsp = 1.541 mg/mm3). The elevated flask and plaster temperature slowed polymer solidification, resulting in longer macromolecules and improved mechanical properties and surface features.
Enhancing Renewable Energy Accommodation by Coupling Integration Optimization with Flexible Retrofitted Thermal Power Plant
Authors: Zou Yao , Xia Qinqin , Chi Yuan , Wang Qianggang
Published: March 2024

Thermal power units (TPUs) play a crucial role in accommodating the high penetration of renewable energy sources (RESs) like wind turbines (WTs) and photovoltaics (PVs). This paper proposes an evaluation framework to quantitatively analyze the flexibility potential of retrofitted TPUs in enhancing the accommodate capability of RESs through coupling integration and optimal scheduling. Firstly, the coordination framework for coupling TPUs with RESs is outlined, including a comprehensive analysis of benefits and implementation strategies. Secondly, an annual optimal scheduling model for TPUs and RESs is developed, incorporating deep peak regulation services, ladder-type constraints for retrofitted TPUs, and their operational characteristics before and after the coupling integration. Thirdly, indices to evaluate RES accommodation levels and TPU regulation capacities are proposed to quantify the performance of power sources. Finally, a real-world case study is conducted to demonstrate that integrating retrofitted TPUs with RESs through coupling significantly enhances RES utilization by 3.6% and boosts TPUs’ downward regulation capabilities by 32%.
Investigation of Cement and Fly Ash on the Improvement of Fine Sand Soil
Authors: Yazıcı Elanur , Unsever Yesim S.
Published: March 2024

Soil stabilization problems like liquefaction, bearing capacity, permeability, excessive settlement and swelling can be solved by improving soil’s engineering properties by using various methods. The use of fly ash as a stabilizer has become popular in recent years since it is eco-friendly and effective on soil stabilization, especially for fine-grained soils. This study investigated the reuse of fly ash as a stabilizer (5–25% by weight) and cement (constant 3% by weight) as an activator to enhance the geotechnical properties of poorly graded sand (SP). Standard proctor tests were conducted to determine optimum water content and maximum dry unit weight, followed by direct shear box, falling head permeability and CBR tests at the determined optimum water content. Direct shear box experiments were carried out at two relative densities (30% and 80%) and CBR experiments were performed after 7 and 28 days of curing time. The results demonstrated that the addition of fly ash and cement improved the geotechnical properties, including shear strength, permeability and bearing capacity of the fine sand soil.
The Impact of the Damping Coefficient on the Dynamic Stability of the TM-AFM Microcantilever Beam System
Authors: Song Peijie , Li Xiaojuan , Cui Jianjun , Chen Kai , Chu Yandong
Published: March 2024

The tapping-mode atomic force microscope (TM-AFM) is widely used today; however, improper matching between the operating medium and the sampling time may lead to inaccurate measurement results. The relationship between the damping coefficient and the steady state of the TM-AFM microcantilever is investigated in this paper using multiple stability theory. Firstly, the effects of changes in dimensionless linear damping coefficients and dimensionless piezoelectric film damping coefficients on the motion stability of the system are examined using bifurcation diagrams, phase trajectories, and domains of attraction. Subsequently, the degrees of effect of the two damping coefficients on the stability of the system are compared. Finally, the bi-parametric bifurcation characteristics of the system under a specific number of iterative cycles are investigated using the bi-parametric bifurcation diagram in conjunction with the actual working conditions, and the boundary conditions for the transition of the system’s motion from an unstable state to a stable state are obtained. The results of the study show that to ensure the accuracy and reliability of the individual measurement data in 500 iteration cycles, the dimensionless linear damping coefficient must be greater than 0.01014. Our results will provide valuable references for TM-AFM measurement media selection, improving TM-AFM imaging quality, measurement accuracy and maneuverability, and TM-AFM troubleshooting.
Isolated Forest-Based Prediction of Container Resource Load Extremes
Authors: Wang Chaoxue , Wang Zhenbang
Published: March 2024

Given the wide application of container technology, the accurate prediction of container CPU usage has become a core aspect of optimizing resource allocation and improving system performance. The high volatility of container CPU utilization, especially the uncertainty of extreme values of CPU utilization, is challenging to accurately predict, which affects the accuracy of the overall prediction model. To address this problem, a container CPU utilization prediction model, called ExtremoNet, which integrates the isolated forest algorithm, and classification sub-models are proposed. To ensure that the prediction model adequately takes into account critical information on the CPU utilization’s extreme values, the isolated forest algorithm is introduced to compute these anomalous extreme values and integrate them as features into the training data. In order to improve the recognition accuracy of normal and extreme CPU utilization values, a classification sub-model is used. The experimental results show that, on the AliCloud dataset, the model has an R2 of 96.51% and an MSE of 7.79. Compared with the single prediction models TCN, LSTM, and GRU, as well as the existing combination models CNN-BiGRU-Attention and CNN-LSTM, the model achieves average reductions in the MSE and MAE of about 38.26% and 23.12%, proving the effectiveness of the model at predicting container CPU utilization, and provides a more accurate basis for resource allocation decisions.
Using Transfer Learning and Radial Basis Function Deep Neural Network Feature Extraction to Upgrade Existing Product Fault Detection Systems for Industry 4.0: A Case Study of a Spring Factory
Authors: Loh Chee-Hoe , Chen Yi-Chung , Su Chwen-Tzeng
Published: March 2024

In the era of Industry 3.0, product fault detection systems became important auxiliary systems for factories. These systems efficiently monitor product quality, and as such, substantial amounts of capital were invested in their development. However, with the arrival of Industry 4.0, high-volume low-mix production modes are gradually being replaced by low-volume high-mix production modes, reducing the applicability of existing systems. The extent of investment has prompted factories to seek upgrades to tailor existing systems to suit new production modes. In this paper, we propose an approach to upgrading based on the concept of transfer learning. The key elements are (1) using a framework with a basic model and an add-on model rather than fine-tuning parameters and (2) designing a radial basis function deep neural network (RBF-DNN) to extract important features to construct the basic and add-on models. The effectiveness of the proposed approach is verified using real-world data from a spring factory.
Stability of a Deep Foundation Pit with Hard Surrounding Rocks under Different in-Time Transverse Supporting Conditions
Authors: Li Yang , Ma Zhanguo , Gao Furong , Gong Peng , Gong Zhiqun , Li Kelong
Published: March 2024

This paper focuses on investigating the stability of a deep foundation pit with hard surrounding rocks at different excavation stages with different supporting schemes by means of numerical calculations. The supporting schemes in question were combinations of one fixed vertical support and four varied transverse supports. Drilled grouting piles were used as vertical supports, and the commonly used steel bracings and prestressed anchorages served as transverse supports. The parameters used to evaluate the stability of the foundation pit at different excavation stages included the lateral displacements of the surrounding rocks, the settlement of the surrounding ground, the axial forces of steel bracings, and displacements at the tops of the drilled grouting piles. Simulation results showed that when a transverse supporting scheme consisting of one-layer steel bracings and prestressed anchorages set at 9 m and 22.5 m underground, respectively, was adopted, the lateral displacements of the surrounding rocks and settlement of the surrounding ground at different excavation stages were the largest compared to those under the other three transverse supporting schemes, while the corresponding values were lower compared to those allowed in Chinese standard GB50007-2011, demonstrating that this kind of supporting scheme is effective in terms of ensuring the safety of the foundation pit at different excavation stages. Moreover, the setting techniques for this kind of supporting scheme were relatively simple, and the corresponding influences of supporting element arrangements on excavation techniques were the lowest. Therefore, one-layer steel bracings and one-layer prestressed anchorages constituted the most suitable transverse supporting scheme for excavating a deep foundation pit with hard surrounding rocks.
Effect of Surface Roughness on Aerodynamic Loads of Bluff Body in Vicinity of Smoothed Moving Wall
Authors: Oliveira Marcos André de , Alcântara Pereira Luiz Antonio
Published: March 2024

This paper contributes to a new Lagrangian vortex method for the statistical control of turbulence in two-dimensional flow configurations around a rough circular cylinder in ground effect when considering higher subcritical Reynolds numbers, namely 3 × 104 ≤ Re ≤ 2 × 105. A smoothed moving wall (active control technique) is used to include the blockage effect in association with the variation in cylinder surface roughness (passive control technique), characterizing a hybrid approach. In contrast with the previous approaches of our research group, the rough cylinder surface is here geometrically constructed, and a new momentum source term is introduced and calculated for the investigated problem. The methodology is structured by coupling the random Discrete Vortex Method, the Lagrangian Dynamic Roughness Model, and the Large Eddy Simulation with turbulence closure using the truncated Second-Order Velocity Structure Function model. This methodological option has the advantage of dispensing with the use of both a refined near-wall mesh and wall functions. The disadvantage of costly processing is readily solved with Open Multi-Processing. The results reveal that intermediate and high roughness values are most efficient for Reynolds numbers on the orders of 105 and 104, respectively. In employing a moving wall, the transition from the large-gap to the intermediate-gap regime is satisfactorily characterized. For the conditions studied with the hybrid technique, it was concluded that the effect of roughness is preponderant and acts to anticipate the characteristics of a lower gap-to-diameter ratio regime, especially with regard to intermittency.
LSTM-Based Autoencoder with Maximal Overlap Discrete Wavelet Transforms Using Lamb Wave for Anomaly Detection in Composites
Authors: Rizvi Syed Haider Mehdi , Abbas Muntazir , Zaidi Syed Sajjad Haider , Tayyab Muhammad , Malik Adil
Published: March 2024

Lamb-wave-based structural health monitoring is widely acknowledged as a reliable method for damage identification, classification, localization and quantification. However, due to the complexity of Lamb wave signals, especially after interacting with structural components and defects, interpreting these waves and extracting useful information about the structure’s health is still a major challenge. Deep-learning-based strategy offers a great opportunity to address such challenges as the algorithm can operate directly on raw discrete time-domain signals. Unlike traditional methods, which often require careful feature engineering and preprocessing, deep learning can automatically extract relevant features from the raw data. This paper proposes an autoencoder based on a bidirectional long short-term memory network (Bi-LSTM) with maximal overlap discrete wavelet transform (MODWT). layer to detect the signal anomaly and determine the location of the damage in the composite structure. MODWT decomposes the signal into multiple levels of detail with different frequency resolution, capturing both temporal and spectral features simultaneously. Comparing with vanilla Bi-LSTM, this approach enables the model to greatly enhance its ability to detect and locate structural damage in structures, thereby increasing safety and efficiency.
Dual-Band Frequency Selective Surface-Backed Reflectarray for High-Speed Ka-Band Satellites
Authors: Gülseren Ahmet Hulusi , Alparslan Aytaç , Türker Tokan Nurhan
Published: March 2024

A dual-band, dual-polarized frequency selective surface (FSS)-backed multilayer reflectarray antenna is designed for 5G high-speed satellites operating at Ka-band uplink and downlink frequencies (20/30 GHz). A reflectarray antenna system consists of two reflectarrays that are separated from each other by an FSS layer that behaves as a planar bandpass filter at Ka-band satellite uplink frequencies. Each reflectarray antenna is designed with dual-polarized unit cells. In order to achieve a uniform phase distribution across the reflectarray surface, physical dimensions and positions of the unit cells with a fixed periodicity are carefully chosen. The FSS conductor is etched to the bottom layer of the 30 GHz reflectarray substrate to save cost and weight. The reflectarray performance is analyzed by using CST Microwave Studio and array theory. A prototype is fabricated, and the results are experimentally verified. The gain of the reflectarray is measured as 21.13 dBi and 26.94 dBi at 20 and 30 GHz, respectively. A crosspol level of more than 35 dB is observed at both frequencies. The simulated and measured results show that the proposed reflectarray is suitable for high-speed Ka-band satellites.