bluuebunny/crossref_metadata_2025 huggingface dataset:
Breeding for Higher Yields of Wheat and Rice through Modifying Nitrogen Metabolism
Authors: Kasemsap Pornpipat , Bloom Arnold J.
Published: December 2022

Wheat and rice produce nutritious grains that provide 32% of the protein in the human diet globally. Here, we examine how genetic modifications to improve assimilation of the inorganic nitrogen forms ammonium and nitrate into protein influence grain yield of these crops. Successful breeding for modified nitrogen metabolism has focused on genes that coordinate nitrogen and carbon metabolism, including those that regulate tillering, heading date, and ammonium assimilation. Gaps in our current understanding include (1) species differences among candidate genes in nitrogen metabolism pathways, (2) the extent to which relative abundance of these nitrogen forms across natural soil environments shape crop responses, and (3) natural variation and genetic architecture of nitrogen-mediated yield improvement. Despite extensive research on the genetics of nitrogen metabolism since the rise of synthetic fertilizers, only a few projects targeting nitrogen pathways have resulted in development of cultivars with higher yields. To continue improving grain yield and quality, breeding strategies need to focus concurrently on both carbon and nitrogen assimilation and consider manipulating genes with smaller effects or that underlie regulatory networks as well as genes directly associated with nitrogen metabolism.
Contrasting Regulators of the Onset and End of the Seed Release Phenology of a Temperate Desert Shrub Nitraria tangutorum
Authors: Bao Fang , Xin Zhiming , Liu Minghu , Li Jiazhu , Gao Ying , Lu Qi , Wu Bo
Published: December 2022

Seed release is crucial in the reproductive cycle of many desert plant species, but their responses to precipitation changes are still unclear. To clarify the response patterns, we conducted a long-term in situ water addition experiment with five treatments, including natural precipitation (control) plus an extra 25%, 50%, 75%, and 100% of the local mean annual precipitation (145 mm), in a temperate desert in northwestern China. Both the onset and end of the seed release phenophase of the locally dominant shrub, Nitraria tangutorum, were observed from 2012 to 2018. The results showed that both the onset and end time of seed release, especially the end time, were significantly affected by water addition treatment. On average, the end time of seed release was advanced by 3.9 d, 7.3 d, 10.8 d, and 3.8 d under +25%, +50%, +75%, and +100% water addition treatments, respectively, over the seven-year study, compared with the control. The changes in the onset time were relatively small (only several hours), and the duration of seed release was shortened by 4.0 d, 7.5 d, 10.8 d, and 2.0 d under +25%, +50%, +75%, and +100% water addition treatments, respectively. The onset and end time of seed release varied greatly between the years. Preceding fruit ripening and summer temperature jointly regulated the inter-annual variation of the onset time of seed release, while the cumulative summer precipitation played a key role in driving the inter-annual variation of the end time. The annual mean temperature controlled the inter-annual variation of the seed release duration, and these interactions were all non-linear.
Two Non-Necrotic Disease Resistance Types Distinctly Affect the Expression of Key Pathogenic Determinants of Xanthomonas euvesicatoria in Pepper
Authors: Bozsó Zoltán , Krüzselyi Dániel , Szatmári Ágnes , Csilléry Gábor , Szarka János , Ott Péter G.
Published: December 2022

Pepper (Capsicum annuum L.) carrying the gds (corresponding to bs5) gene can prevent the development of bacterial leaf spot disease without HR. However, little is known regarding the development of the resistance mechanism encoded by gds, especially its influence on the bacterium. Here, the effect of gds was compared with pattern-triggered immunity (PTI), another form of asymptomatic resistance, to reveal the interactions and differences between these two defense mechanisms. The level of resistance was examined by its effect on the bacterial growth and in planta expression of the stress and pathogenicity genes of Xanthomonas euvesicatoria. PTI, which was activated with a Pseudomonas syringae hrcC mutant pretreatment, inhibited the growth of Xanthomonas euvesicatoria to a greater extent than gds, and the effect was additive when PTI was activated in gds plants. The stronger influence of PTI was further supported by the expression pattern of the dpsA bacterial stress gene, which reached its highest expression level in PTI-induced plants. PTI inhibited the hrp/hrc expression, but unexpectedly, in gds plant leaves, the hrp/hrc genes were generally expressed at a higher level than in the susceptible one. These results imply that different mechanisms underlie the gds and PTI to perform the symptomless defense reaction.
Capacity of Nerium oleander to Phytoremediate Sb-Contaminated Soils Assisted by Organic Acids and Oxygen Nanobubbles
Authors: Seridou Petroula , Monogyiou Sofia , Syranidou Evdokia , Kalogerakis Nicolas
Published: December 2022

Antimony (Sb) is considered to be a toxic metalloid of increasing prevalence in the environment. Although several phytoremediation studies have been conducted, research regarding the mechanisms of Sb accumulation and translocation within plants remains limited. In this study, soil from a shooting range was collected and spiked with an initial Sb(III) concentration of 50 mg/kg. A pot experiment was conducted to investigate whether Nerium oleander could accumulate Sb in the root and further translocate it to the aboveground tissue. Biostimulation of the soil was performed by the addition of organic acids (OAs), consisting of citric, ascorbic, and oxalic acid at low (7 mmol/kg) or high (70 mmol/kg) concentrations. The impact of irrigation with water supplemented with oxygen nanobubbles (O2NBs) was also investigated. The results demonstrate that there was a loss in plant growth in all treatments and the presence of OAs and O2NBs assisted the plant to maintain the water content at the level close to the control. The plant was not affected with regards to chlorophyll content in all treatments, while the antioxidant enzyme activity of guaiacol peroxidase (GPOD) in the roots was found to be significantly higher in the presence of Sb. Results revealed that Sb accumulation was greater in the treatment with the highest OAs concentration, with a bioconcentration factor greater than 1.0. The translocation of Sb for every treatment was very low, confirming that N. oleander plant cannot transfer Sb from the root to the shoots. A higher amount of Sb was accumulated in the plants that were irrigated with the O2NBs, although the translocation of Sb was not increased. The present study provides evidence for the phytoremediation capacity of N. oleander to bioaccumulate Sb when assisted by biostimulation with OAs.
Photosynthetic Plasticity and Stomata Adjustment in Chromosome Segment Substitution Lines of Rice Cultivar KDML105 under Drought Stress
Authors: Lertngim Narawitch , Ruangsiri Mathurada , Klinsawang Suparad , Raksatikan Pimpa , Thunnom Burin , Siangliw Meechai , Toojinda Theerayut , Siangliw Jonaliza Lanceras
Published: December 2022

The impact of increasing drought periods on crop yields as a result of global climate change is a major concern in modern agriculture. Thus, a greater understanding of crop physiological responses under drought stress can guide breeders to develop new cultivars with enhanced drought tolerance. In this study, selected chromosome segment substitution lines of KDML105 (KDML105-CSSL) were grown in the Plant Phenomics Center of Kasetsart University in Thailand under well-watered and drought-stressed conditions. Physiological traits were measured by observing gas exchange dynamics and using a high-throughput phenotyping platform. Furthermore, because of its impact on plant internal gas and water regulation, stomatal morphological trait variation was recorded. The results show that KDML105-CSS lines exhibited plasticity responses to enhance water-use efficiency which increased by 3.62%. Moreover, photosynthesis, stomatal conductance and transpiration decreased by approximately 40% and plant height was reduced by 17.69%. Stomatal density tended to decrease and was negatively correlated with stomatal size, and stomata on different sides of the leaves responded differently under drought stress. Under drought stress, top-performing KDML105-CSS lines with high net photosynthesis had shorter plant height and improved IWUE, as influenced by an increase in stomatal density on the upper leaf side and a decrease on the lower leaf side.
The Effect of Different Agrotechnical Treatments on the Establishment of Miscanthus Hybrids in Soil Contaminated with Trace Metals
Authors: Krzyżak Jacek , Rusinowski Szymon , Sitko Krzysztof , Szada-Borzyszkowska Alicja , Stec Radosław , Jensen Elaine , Clifton-Brown John , Kiesel Andreas , Lewin Eva , Janota Paulina , Pogrzeba Marta
Published: December 2022

Climate change and man-made pollution can have a negative impact on the establishment of Miscanthus plants in the field. This is particularly important because biomass can be produced on marginal land without conflicting with food crops. The establishment success depends on the hybrid chosen, the cultivation method, the climatic conditions, and the concentration of pollutants in the soil. There are several ways to increase the survival rate of the plants during the first growing season and after the first winter. One of them is the application of biochar and photodegradable plastic mulch, which can provide a solution for soils polluted with trace elements (TMEs). The aim of this study was to investigate the application of plastic mulch and biochar separately and in combination at the planting stage for two Miscanthus hybrids planted by the rhizome method (TV1) and seedling plugs (GNT43) on soils contaminated with trace metal elements (Pb, Cd, Zn). TV1 seems unsuitable for TME-contaminated field cultivation, as the survival rate was <60% in most treatments studied. The selected treatments did not increase the survival rate. Furthermore, the application of plastic mulch in combination with biochar resulted in a significant reduction of this parameter, regardless of the hybrid studied. The applied agrotechnology did not influence the TME accumulation in the aboveground plant parts in TV1, while Pb and Cd in GNT43 showed significantly higher values in all treatments. Contrary to expectations, biochar and plastic mulch applied separately and together neither increased survival nor reduced the accumulation of toxic TMEs during establishment on soil contaminated with TMEs and after the first growing season.
Chemical and Biological Evaluation of Amazonian Medicinal Plant Vouacapoua americana Aubl
Authors: Çiçek Serhat Sezai , Pfeifer Barbosa Anna Laís , Wenzel-Storjohann Arlette , Segovia Jorge Federico Orellana , Bezerra Roberto Messias , Sönnichsen Frank , Zidorn Christian , Kanzaki Isamu , Tasdemir Deniz
Published: December 2022

Vouacapoua americana (Fabaceae) is an economically important tree in the Amazon region and used for its highly resistant heartwood as well as for medicinal purposes. Despite its frequent use, phytochemical investigations have been limited and rather focused on ecological properties than on its pharmacological potential. In this study, we investigated the phytochemistry and bioactivity of V. americana stem bark extract and its constituents to identify eventual lead structures for further drug development. Applying hydrodistillation and subsequent GC-MS analysis, we investigated the composition of the essential oil and identified the 15 most abundant components. Moreover, the diterpenoids deacetylchagresnone (1), cassa-13(14),15-dien-oic acid (2), isoneocaesalpin H (3), (+)-vouacapenic acid (4), and (+)-methyl vouacapenate (5) were isolated from the stem bark, with compounds 2 and 4 showing pronounced effects on Methicillin-resistant Staphylococcus aureus and Enterococcus faecium, respectively. During the structure elucidation of deacetylchagresnone (1), which was isolated from a natural source for the first time, we detected inconsistencies regarding the configuration of the cyclopropane ring. Thus, the structure was revised for both deacetylchagresnone (1) and the previously isolated chagresnone. Following our works on Copaifera reticulata and Vatairea guianensis, the results of this study further contribute to the knowledge of Amazonian medicinal plants.
The Phenolic Profile of Sweet Cherry Fruits Influenced by Cultivar/Rootstock Combination
Authors: Boskov Djordje , Milatovic Dragan , Rakonjac Vera , Zec Gordan , Hudina Metka , Veberic Robert , Mikulic-Petkovsek Maja
Published: December 2022

The influence of three cultivars (‘Carmen’, ‘Kordia’ and ‘Regina’) grafted on six rootstocks (Mahaleb, ‘Colt’, ‘Oblacinska’, ‘M × M 14′, ‘Gisela 5′ and ‘Gisela 6′) on the phenolic profile of sweet cherry fruits was studied during a two-year period. All the individual phenolic compounds were detected using high-pressure liquid chromatography with diode-array detection coupled with mass spectrometry (HPLC-DAD-MSn). In all the examined samples, 54 compounds were identified and divided into five phenolic classes: anthocyanins (4 compounds), flavonols (7), flavanols (11), flavanones (4), and hydroxycinnamic acids (28). Anthocyanins (58%) and hydroxycinnamic acids (31%) showed the greatest amounts in all the examined fruit samples. PCA analysis revealed that among the cultivars, ‘Kordia’ showed the highest phenolic content. Regarding rootstocks, the lowest values of the most important phenolic compounds were obtained in fruits from trees grafted onto the seedling rootstock Mahaleb. Among the clonal rootstocks, the vigorous ‘Colt’ and dwarf ‘Gisela 5′ promoted the highest values of the evaluated phenolic compounds in the cultivars ‘Kordia’ and ‘Carmen’, while the dwarf ‘Oblacinska’ and semi-vigorous ‘M × M 14′ induced the highest values in the cultivar ‘Regina’. By evaluating the influence of cultivars and rootstocks on the phenolic content in fruit, it has been proven that the cultivar has the most significant influence. However, the rootstock also influences the content of a large number of phenolic compounds. The selection of an adequate cultivar/rootstock combination can also be a powerful tool for improving the phenolic content in fruits, and consequently the nutritional value of sweet cherry fruits.
Design of Intelligent Detection Platform for Wine Grape Pests and Diseases in Ningxia
Authors: Wang Yutan , Wei Chi , Sun Haowei , Qu Aili
Published: December 2022

In order to reduce the impact of pests and diseases on the yield and quality of Ningxia wine grapes and to improve the efficiency and intelligence of detection, this paper designs an intelligent detection platform for pests and diseases. The optimal underlying network is selected by comparing the recognition accuracy of both MobileNet V2 and YOLOX_s networks trained on the Public Dataset. Based on this network, the effect of adding attention mechanism and replacing loss function on recognition effect is investigated by permutation in the Custom Dataset, resulting in the improved network YOLOX_s + CBAM. The improved network was trained on the Overall Dataset, and finally a recognition model capable of identifying nine types of pests was obtained, with a recognition accuracy of 93.35% in the validation set, an improvement of 1.35% over the original network. The recognition model is deployed on the Web side and Raspberry Pi to achieve independent detection functions; the channel between the two platforms is built through Ngrok, and remote interconnection is achieved through VNC desktop. Users can choose to upload local images on the Web side for detection, handheld Raspberry Pi for field detection, or Raspberry Pi and Web interconnection for remote detection.
Influence of Spraying Nano-Curcumin and Nano-Glycyrrhizic Acid on Resistance Enhancement and Some Growth Parameters of Soybean (Glycine max) in Response to Tetranychus urticae Infestation and Drought Stress
Authors: Salama Azza M. , Ramadan Ahmed M. , Alakhdar Hala H. , Khan Thana K. , El-Garhy Hoda A. S. , Shoala Tahsin
Published: December 2022

Modern nanotechnology has been credited as one of the most significant inventions of the 21st century. Many agricultural disciplines have been affected by nanotechnology in agriculture. Pest control based on natural compounds needs to be enhanced, and enhancing plant growth under climate change conditions, with increasing periods of drought in many countries, is a very vital aim. Thus, the effect of curcumin nanoparticles (Cu-NPs) and glycyrrhizic acid nanoparticles (GA-NPs) as a foliar application under water deficit on natural infestation with the two-spotted spider mite Tetranychus urticae, plant growth and yield, anatomical and chemical parameters were investigated during this study. The obtained results revealed that drought stress over the two studied seasons significantly increased the population of T. urticae and decreased all morphological and yield characteristics. The application of three mM GA-NPs reduced the mite population average by 39% while using the same concentration of Cu-NPs caused a 33.9% reduction percentage under drought stress. Using 1 mM GA-NPs gave the highest averages of plant height, number of branches, and leaves/plant fresh and dry weight. Moreover, the number of pods, 100 seed weight and seed yield (kg/ha) increased significantly as a result of spraying with GA-NPs under water deficit. From the results, water deficit decreased the values of the leaf and stem anatomical parameters. Treatment with Cu-NPs or GA-NPs under drought stress increased the thickness of mid-vein, xylem, and phloem tissues. Likewise, such treatment increased stem diameter due mainly to the increase in the thickness of cortex, phloem, and xylem tissues compared with the control. Spraying plants with GA-NPs at 1 mM increased the percentages of nitrogen, phosphor, and potassium in seeds in addition to total chlorophyll. Moreover, glutamate, aspartate, leucine, arginine, Lysine, glycine, tyrosine, tryptophan, and methionine concentrations did not differ significantly (p > 0.05) in response to all the studied levels of Cu-NPs or GA-NPs either under normal irrigation or drought condition. In light of these findings, researchers and producers should apply and test both Cu-NPs and GA-NP as nano-fertilizer natural sources on economically viable crops.
Meta-QTL Analysis for Yield Components in Common Bean (Phaseolus vulgaris L.)
Authors: Arriagada Osvin , Arévalo Bárbara , Cabeza Ricardo A. , Carrasco Basilio , Schwember Andrés R.
Published: December 2022

Common bean is one of the most important legumes produced and consumed worldwide because it is a highly valuable food for the human diet. However, its production is mainly carried out by small farmers, who obtain average grain yields below the potential yield of the species. In this sense, numerous mapping studies have been conducted to identify quantitative trait loci (QTL) associated with yield components in common bean. Meta-QTL (MQTL) analysis is a useful approach to combine data sets and for creating consensus positions for the QTL detected in independent studies. Consequently, the objective of this study was to perform a MQTL analysis to identify the most reliable and stable genomic regions associated with yield-related traits of common bean. A total of 667 QTL associated with yield-related traits reported in 21 different studies were collected. A total of 42 MQTL associated with yield-related traits were identified, in which the average confidence interval (CI) of the MQTL was 3.41 times lower than the CIs of the original QTL. Most of the MQTL (28) identified in this study contain QTL associated with yield and phenological traits; therefore, these MQTL can be useful in common bean breeding programs. Finally, a total of 18 candidate genes were identified and associated with grain yield within these MQTL, with functions related to ubiquitin ligase complex, response to auxin, and translation elongation factor activity.
Antimicrobial Properties of Different Hop (Humulus lupulus) Genotypes
Authors: Kolenc Zala , Langerholc Tomaž , Hostnik Gregor , Ocvirk Miha , Štumpf Sara , Pintarič Maša , Košir Iztok Jože , Čerenak Andreja , Garmut Alenka , Bren Urban
Published: December 2022

The antimicrobial activity of hop extracts obtained from different hop genotypes were investigated against Staphylococcus aureus and Lactobacillus acidophilus. In this study the pure xanthohumol, purified β-acids rich fraction, as well as α-acids with β-acids rich fraction were used to test antimicrobial activity against Staphylococcus aureus and Lactobacillus acidophilus; whereby, the antimicrobial activity of different hop extracts against Lactobacillus acidophilus was studied for the first time. Microbial susceptibility to purified hydroacetonic extracts from different hop varieties was investigated by the broth microdilution assay to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The hop hydroacetonic extracts were more effective against Staphylococcus aureus than against Lactobacillus acidophilus. Strong inverse correlations of MIC and MBC values were obtained with xanthohumol, cohumulone, n+adhumulone, colupulone and n+adlupulone contents, suggesting that the identified chemical hop compounds are directly responsible for antimicrobial effects. Moreover, the effect of the growth medium strength on the MIC values of hop extracts against Staphylococcus aureus was systematically investigated for the first time. The current study also reveals the effect of different hop extracts on Staphylococcus aureus, which responds to their presence by lag phase extension and generation time prolongation.
Effects of Long-Term Use of Organic Fertilizer with Different Dosages on Soil Improvement, Nitrogen Transformation, Tea Yield and Quality in Acidified Tea Plantations
Authors: Ye Jianghua , Wang Yuhua , Kang Jiaqian , Chen Yiling , Hong Lei , Li Mingzhe , Jia Yun , Wang Yuchao , Jia Xiaoli , Wu Zeyan , Wang Haibin
Published: December 2022

In this study, sheep manure fertilizers with different dosages were used for five consecutive years to treat acidified tea plantation soils, and the effects of sheep manure fertilizer on soil pH value, nitrogen transformation, and tea yield and quality were analyzed. The results showed that soil pH value showed an increasing trend after a continuous use of sheep manure fertilizer from 2018 to 2022. After the use of low dosage of sheep manure fertilizer (6 t/hm2–15 t/hm2), tea yield, the content of tea quality indicators (tea polyphenols, theanine, amino acid, and caffeine) and soil ammonium nitrogen content, ammoniating bacteria number, ammoniating intensity, urease activity and protease activity showed increasing trends and were significantly and positively correlated to soil pH value, while the related indexes showed increasing and then decreasing trends after the use of high dosage of sheep manure fertilizer (18 t/hm2). Secondly, the nitrate nitrogen content, nitrifying bacteria number, nitrifying intensity, nitrate reductase activity, and nitrite reductase activity showed decreasing trends after the use of low dosage of sheep manure fertilizer and showed significant negative correlations with soil pH value, while the related indexes showed decreasing trends after the use of high dosage of sheep manure and then increased. The results of principal component and interaction analysis showed that the effects of sheep manure fertilizers with different dosages on tea yield and quality were mainly based on the transformation ability of ammonium nitrogen and nitrate nitrogen in the soil, and the strong transformation ability of ammonium nitrogen and the high ammonium nitrogen content in the soil were conducive to the improvement of tea yield and quality, and vice versa. The results of topsis comprehensive evaluation and analysis showed that the most influential effect on the fertilization effect was the ammonium nitrogen content in the soil and long-term treatment with 15 t/hm2 of sheep manure fertilizer had the highest proximity to the best fertilization effect. This study provided an important practical basis for the remediation and fertilizer management in acidified tea plantation soils.
Uses, Knowledge and Extinction Risk Faced by Agave Species in Mexico
Authors: Alducin-Martínez Cecilia , Ruiz Mondragón Karen Y. , Jiménez-Barrón Ofelia , Aguirre-Planter Erika , Gasca-Pineda Jaime , Eguiarte Luis E. , Medellin Rodrigo A.
Published: December 2022

We compiled an updated database of all Agave species found in Mexico and analyzed it with specific criteria according to their biological parameters to evaluate the conservation and knowledge status of each species. Analyzing the present status of all Agave species not only provides crucial information for each species, but also helps determine which ones require special protection, especially those which are heavily used or cultivated for the production of distilled beverages. We conducted an extensive literature review search and compiled the conservation status of each species using mainstream criteria by IUCN. The information gaps in the database indicate a lack of knowledge and research regarding specific Agave species and it validates the need to conduct more studies on this genus. In total, 168 Agave species were included in our study, from which 89 are in the subgenus Agave and 79 in the subgenus Littaea. Agave lurida and A. nizandensis, in the subgenus Agave and Littaea, respectively, are severely endangered, due to their endemism, lack of knowledge about pollinators and floral visitors, and their endangered status according to the IUCN Red List. Some species are at risk due to the loss of genetic diversity resulting from production practices (i.e., Agave tequilana), and others because of excessive and unchecked overharvesting of wild plants, such as A. guadalajarana, A. victoriae-reginae, A. kristenii, and others. Given the huge economic and ecological importance of plants in the genus Agave, our review will be a milestone to ensure their future and continued provision of ecosystem services for humans, as well as encouraging further research in Agave species in an effort to enhance awareness of their conservation needs and sustainable use, and the implementation of eco-friendly practices in the species management.
Non-Additive Effects of Environmental Factors on Growth and Physiology of Invasive Solidago canadensis and a Co-Occurring Native Species (Artemisia argyi)
Authors: Yang Bin , Cui Miaomiao , Dai Zhicong , Li Jian , Yu Haochen , Fan Xue , Rutherford Susan , Du Daolin
Published: December 2022

Changes in environmental factors, such as temperature and UV, have significant impacts on the growth and development of both native and invasive plant species. However, few studies examine the combined effects of warming and enhanced UV on plant growth and performance in invasive species. Here, we investigated single and combined effects of warming and UV radiation on growth, leaf functional and photosynthesis traits, and nutrient content (i.e., total organic carbon, nitrogen and phosphorous) of invasive Solidago canadensis and its co-occurring native species, Artemisia argyi, when grown in culture racks in the greenhouse. The species were grown in monoculture and together in a mixed community, with and without warming, and with and without increased UV in a full factorial design. We found that growth in S. canadensis and A. argyi were inhibited and more affected by warming than UV-B radiation. Additionally, there were both antagonistic and synergistic interactions between warming and UV-B on growth and performance in both species. Overall, our results suggested that S. canadensis was more tolerant to elevated temperatures and high UV radiation compared to the native species. Therefore, substantial increases in temperature and UV-B may favour invasive S. canadensis over native A. argyi. Research focusing on the effects of a wider range of temperatures and UV levels is required to improve our understanding of the responses of these two species to greater environmental variability and the impacts of climate change.
CO2 Levels Modulate Carbon Utilization, Energy Levels and Inositol Polyphosphate Profile in Chlorella
Authors: Morales-Pineda María , García-Gómez Maria Elena , Bedera-García Rodrigo , García-González Mercedes , Couso Inmaculada
Published: December 2022

Microalgae have a growing recognition of generating biomass and capturing carbon in the form of CO2. The genus Chlorella has especially attracted scientists’ attention due to its versatility in algal mass cultivation systems and its potential in mitigating CO2. However, some aspects of how these green microorganisms respond to increasing concentrations of CO2 remain unclear. In this work, we analyzed Chlorella sorokiniana and Chlorella vulgaris cells under low and high CO2 levels. We monitored different processes related to carbon flux from photosynthetic capacity to carbon sinks. Our data indicate that high concentration of CO2 favors growth and photosynthetic capacity of the two Chlorella strains. Different metabolites related to the tricarboxylic acid cycle and ATP levels also increased under high CO2 concentrations in Chlorella sorokiniana, reaching up to two-fold compared to low CO2 conditions. The signaling molecules, inositol polyphosphates, that regulate photosynthetic capacity in green microalgae were also affected by the CO2 levels, showing a deep profile modification of the inositol polyphosphates that over-accumulated by up to 50% in high CO2 versus low CO2 conditions. InsP4 and InsP6 increased 3- and 0.8-fold, respectively, in Chlorella sorokiniana after being subjected to 5% CO2 condition. These data indicate that the availability of CO2 could control carbon flux from photosynthesis to carbon storage and impact cell signaling integration and energy levels in these green cells. The presented results support the importance of further investigating the connections between carbon assimilation and cell signaling by polyphosphate inositols in microalgae to optimize their biotechnological applications.
Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves
Authors: Malú Quintino , Lima Katelene , Malmir Maryam , Pinto Rui , da Silva Isabel Moreira , Catarino Luís , Duarte Maria Paula , Serrano Rita , Rocha João , Lima Beatriz Silva , Silva Olga
Published: December 2022

Dried leaves of Lannea velutina A. Rich. and Sorindeia juglandifolia (A. Rich.) Planch. ex Oliv. (family Anacardiaceae) are used in African traditional medicine. Although these medicinal plants have widespread use in the treatment of inflammatory diseases, there is no scientific data concerning their preclinical or clinical safety. This work aimed to investigate the phytochemical properties of the leaves of both species using HPLC-UV/DAD, as well as the in vivo oral repeated-dose toxicity of 70% hydroethanolic leaf extract of S. juglandifolia and the in vitro genotoxicity of 70% hydroethanolic leaf extracts of L. velutina and S. juglandifolia. Clinical signs of toxicity, body weight variations, and changes in food consumption, mortality, and blood biochemical parameters were monitored. Genotoxicity was assessed using the bacterial reverse mutation assay (Ames test) with and without metabolic activation, according to OECD guidelines. The obtained results showed the presence of gallic acid and anacardic acid as the main marker constituents in both species. No significant changes in general body weight or food intake were observed; small significant changes with no critical relevance were observed in the blood biochemistry of animals treated with S. juglandifolia hydroethanolic extract (50, 400, and 1000 mg/kg body weight) compared to those in the control group. No genotoxicity was observed in the bacterial reverse mutation assay with S. juglandifolia and L. velutina extracts (up to 5 mg/plate). The safety data obtained in vivo and lack of genotoxic potential in vitro points to the safe medicinal use of S. juglandifolia and L. velutina extracts.
CFD Analysis of Mixing Process of Detergents in Rotational and Displacement Vessels
Authors: Domingo Jerónimo , Iranzo Alfredo , Arnanz David , Srivastava Akhilesh K. , Groombridge Michael , Hansen Jared
Published: December 2022

As part of the European Commission research project DIY4U focused on the development of machinery to be installed in supermarket allowing customers to define their customized detergent according to their needs. These machines will mix the detergent components (surfactant, fatty acid, water, perfume, etc.) already in the detergent canister as sold to consumers. To avoid long waiting times for customers, and to obtain a product with good quality and consistency, mixing must be very efficient. A mixing process with rotation and displacement by means of rotating the canister around an axis below the canister bottom has been checked by means of Computational Fluid Dynamics (CFD) tools after validation of one case with lab results. This is a new approach for liquid detergents, as commonly is a powder detergent production process. The mixing process has been simulated for 39 different combinations of components mass fraction percentages and the mixing quality observed during the mixing period. A response surface obtained from these simulations has been developed to be included in a Digital Twin, this being a task within this DIY4U project. The results show that this system is very efficient, taking a few seconds to develop a complete mixing. Also, the mixing time differences are quite small, requiring all customers to wait just few seconds independently of their detergent formulation.
Scenario-Driven Methodology for Cascading Disasters Risk Assessment of Earthquake on Chemical Industrial Park
Authors: Guo Li , Liang Junming , Chen Tao , Gao Yuan , Yang Zhen
Published: December 2022

With the increase in industrial accidents induced by natural disasters, the study of earthquake risk assessment has been widely considered by scholars. However, the cascade evolution of Natech (natural–technological) disasters has not been thoroughly studied, especially in chemical parks with complex technological processes. From the perspective of scenario deduction, combined with cross-impact analysis and a damping interpretation structural model, this paper analyzes the evolution process of cascade disaster in a chemical industrial park after the Wenchuan earthquake. At the same time, a visual network risk assessment model is constructed to identify the impact of earthquake cascade disasters on the park. The simulation results show that the scenario-driven risk assessment method proposed in this paper can directly reflect the coupling relationship and propagation path among the derived events and realize dynamic, intuitive and structured disaster expression to deal with the earthquake Natech (natural–technological) disaster scenario effectively and quickly.
Assessing Long-Term Medical Remanufacturing Emissions with Life Cycle Analysis
Authors: Meister Julia A. , Sharp Jack , Wang Yan , Nguyen Khuong An
Published: December 2022

The unsustainable take-make-dispose linear economy prevalent in healthcare contributes 4.4% to global Greenhouse Gas emissions. A popular but not yet widely-embraced solution is to remanufacture common single-use medical devices like electrophysiology catheters, significantly extending their lifetimes by enabling a circular life cycle. To support the adoption of catheter remanufacturing, we propose a comprehensive emission framework and carry out a holistic evaluation of virgin manufactured and remanufactured carbon emissions with Life Cycle Analysis (LCA). We followed ISO modelling standards and NHS reporting guidelines to ensure industry relevance. We conclude that remanufacturing may lead to a reduction of up to 60% per turn (−1.92 kg CO2eq, burden-free) and 57% per life (−1.87 kg CO2eq, burdened). Our extensive sensitivity analysis and industry-informed buy-back scheme simulation revealed long-term emission reductions of up to 48% per remanufactured catheter life (−1.73 kg CO2eq). Our comprehensive results encourage the adoption of electrophysiology catheter remanufacturing, and highlight the importance of estimating long-term emissions in addition to traditional emission metrics.
Density Functional Theory Study on the Adsorption of Fe(OH)2+ on Kaolinite Surface in Water Environment
Authors: Wu Hongqiang , Miao Yuqi , Li Yong , Yan Huashan , Tan Jinbiao , Qiu Sen , Wu Hao , Qiu Tingsheng
Published: December 2022

Fe impurity is abundant in rare earth leaching solutions. The optimal hydrate structure of Fe(OH)2+ was calculated based on the quantum chemical in the water environment to investigate the microscopic occurrence of Fe impurity on kaolinite surfaces. The adsorption structure and bonding mechanism (including outer and inner layer) of hydrate Fe(OH)2+ on the kaolinite (001) surface were investigated. According to the results, the stable hydrate form of Fe(OH)2+ is [Fe(OH)(H2O)5]2+. Hydrated Fe(OH)2+ has a tendency to adhere to the Si-O surface in the form of outer layer adsorption. Adsorbate tends to adsorb to the Ou (deprotonated upright hydroxyl) site, where it generates a monodentate adsorption compound, and to the Ol and Ou (deprotonated lying and upright hydroxyl) sites, where it generates a bidentate adsorption compound if inner layer adsorption occurs. The Mulliken population and density of state analysis demonstrate that the ionic properties of Fe-Os in the inner layer adsorption compound are reduced and Fe-Os bonds are filled with strong bonding and weak antibonding orbitals. More chemical bonds are formed in the bidentate adsorption compound, and the bidentate adsorption has lower adsorption energy. Therefore, in the process of inner layer adsorption, bidentate adsorption is more likely to occur.
Development of a SPE-HPLC-PDA Method for the Quantification of Phthalates in Bottled Water and Their Gene Expression Modulation in a Human Intestinal Cell Model
Authors: Ferrone Vincenzo , Bruni Pantaleone , Catalano Teresa , Selvaggi Federico , Cotellese Roberto , Carlucci Giuseppe , Aceto Gitana Maria
Published: December 2022

Phthalates are ubiquitous pollutants that are currently classified as endocrine disruptor chemicals causing serious health problems. As contaminants of food and beverages, they come into contact with the epithelium of the intestinal tract. In this work, a SPE-HPLC-PDA method for the determination of phthalates in water from plastic bottles was developed and validated according to the food and drug administration (FDA) guidelines. A chromatographic separation was achieved using a mobile phase consisting of ammonium acetate buffer 10 mM pH 5 (line A) and a mixture of methanol and iso-propanol (50:50 v/v, line B) using gradient elution. Several SPE cartridges and different pH values were investigated for this study, evaluating their performance as a function of recovery. Among these parameters, pH 5 combined with the SPE sep pack C18 cartridge showed the best performance. Finally, the proposed method was applied to the analysis of real samples, which confirmed the presence of phthalates. A colonic epithelial cell model was used to evaluate the effects of these phthalates at the concentrations found in water from plastic bottles. In cells exposed to phthalates, the increased expression of factors, which control the signaling pathways necessary for intestinal epithelium homeostasis, inflammatory response, and stress was detected. The proposed method falls fully within the limits imposed by the guidelines with precision (RSD%) below 7.1% and accuracy (BIAS%) within −4.2 and +6.1.
Computational Fluid Dynamics Modeling of Single Isothermal and Non-Isothermal Impinging Jets in a Scaled-Down High-Temperature Gas-Cooled Reactor Facility
Authors: Alwafi Anas M. , Alshehri Salman M. , Alzahrani Salman M.
Published: December 2022

In the current work, the flow characteristics of single isothermal and non-isothermal jets discharging into the upper plenum of a 1/16th scaled-down high-temperature gas-cooled reactor (HTGR) facility were studied. ANSYS Fluent simulations were carried out in the central plane of the jet water flow and the upper plenum for different Reynolds numbers (Re) ranging from 3413 to 12,819. Then, the statistical jet water flow characteristics, such as the mean velocity, root-mean-square fluctuating velocity, Reynolds stress, and the mean temperature in the upper plenum, were computed and presented. The current study’s results showed that the flow maximum velocity occurred far from the jet inlet. Finally, the temperature profiles were plotted, and it was found that the maximum temperature of the flow occurred close to the plume inlet and after that decreased downstream.
Numerical Simulations of Particle Motions at Continuous Rotational Speed Changes in Horizontal Rotating Drums
Authors: Zhao Yuze , Zhang Lidong , Song Changpeng , Li Weiwei , Qin Hong , Wang Qing
Published: December 2022

The motion of binary particles in three horizontal rotating drums with continuous rotational speed changes was studied based on the Discrete Element Method (DEM). Different simulation conditions were compared between two circular drums and an elliptical drum using the same number of physical properties for binary particles and drums, rotating at a speed series from 0.01 to 21.9 rad/s. By varying the rotational speed, four flow regimes were produced in the simulation. Flow regimes, velocity vectors, normal forces, and the number of contacts between 1 mm particles and 3 mm particles were comparatively analyzed, especially the particle velocity at transient changing rotational speeds. The results showed that four flow regimes were found at the same rotational speed for three different rotating drums, and normal forces were weakest for the cataracting regime; moreover, the three layers of particles were damaged when the rotational speed was suddenly decreased and the velocity direction of the particle motion was changed at the top of the particles’ bed. The maximum number of contacts was found with the rolling regime, based on the simulation results. The number of contacts of the major axis circular drum was smaller than for the minor axis at the same rotational speed, and the number of contacts of the elliptical drum was the largest among the three rotating drums.
A Simultaneous Design and Optimization Framework for the Reaction and Distillation Sections of Methanol to Olefins Process
Authors: Li Ning , Zhao Liwen , Li Dan , Sun Huifeng , Zhang Di , Liu Guilian
Published: December 2022

The reaction and separation sections are the keys to the methanol-to-olefins (MTO) chemical processes, and they should be optimized to reduce the cost of production. This work develops a framework for the simultaneous design and optimization of the reaction and distillation sections. An optimization model with shortcut and rigorous methods combined is established for distillation columns to improve accuracy and efficiency. With the auxiliary devices and the selection of utilities considered, the reaction and distillation sections are integrated to maximize profits. The genetic algorithm targets the optimal parameters, including the catalyst’s coke content and reaction temperature, each column’s operating pressure, and the allocation of utilities and auxiliary devices. For the studied MTO process, the optimal reaction temperature and catalyst’s coke content were identified to be 496 °C and 7.8%, respectively. The maximum profit is 15.3% greater than that identified with only the separation section optimized, and the minimum total annual cost (TAC) of the separation section is 3.73% less.
Mental Health Risk Factors Related to COVID-19 among Canadian Public Safety Professionals
Authors: Wagner Shannon , Di Nota Paula M. , Groll Dianne , Lentz Liana , Shields Robyn E. , Carleton R. Nicholas , Cramm Heidi , Wei Lin Becky , Anderson Gregory S.
Published: December 2022

Public safety personnel (PSP) are known to experience difficult and demanding occupational environments, an environment that has been complicated by the COVID-19 pandemic. Firefighters, paramedics, and public safety communicators were among the front-line workers that continued to serve the public throughout the course of the pandemic. The present study considered the potential impacts of the COVID-19 pandemic on self-reported symptoms of mental health challenges in Canadian firefighters, paramedics, and public safety communicators. Participants were firefighters (n = 123), paramedics (n = 246), and public safety communicators (n = 48), who completed an online survey, including demographics, questions related to COVID-19 exposure and worry, the Patient Health Questionnaire-9, the Generalized Anxiety Disorder-7, the Social Interaction Phobia Scale, and the Posttraumatic Stress Disorder Checklist-5. Results revealed that risk factors for increased mental health symptom reporting were paramedic occupation, self-identified female, younger in age, COVID-19 personal contact, requirement to self-isolate, and self-perception of COVID-19 contraction (without confirmation through testing). The COVID-19 pandemic should be considered a risk factor for increased mental health symptom reporting in PSP.
A Compensatory Response to the Problem of Evil: Revisited
Authors: Beaty Michael Douglas
Published: December 2022

In this essay, I revisit the univocity thesis, Sterba’s analogy between God and a leader of a politically liberal society, and, most fundamentally, whether the existence of horrendous evils is logically compatible with the existence of a good God. I concede that the typical appeals to free will and greater goods defenses to block the logical problem of evil are not sufficient because they do not adequately address the horrendous evils that are all too much a feature of human existence. While acknowledging that a compensatory response to the problem of evil is suggested by several important philosophers, I rely most centrally on the work of Marilyn McCord Adams. In so doing, I defend the thesis that the existence of a good God is logically compatible with the existence of horrendous evils, given God’s capacity to absorb, defeat, or engulf it.