bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Trehalose/Hyaluronic Acid (HA) vs. 0.001% Hydrocortisone/HA Eyedrops on Signs and Inflammatory Markers in a Desiccating Model of Dry Eye Disease (DED)
Authors: Astolfi Gloria , Lorenzini Luca , Gobbo Francesca , Sarli Giuseppe , Versura Piera
Published: March 2022

Background: Dry eye disease (DED) is a multifactorial disease where ocular surface inflammation and damage play key etiological roles. Purpose: To compare a combination of 3% trehalose (T) and 0.15% hyaluronic acid (HA) (Thealoz duo®, T/HA) with a tear substitute containing 0.001% hydrocortisone (I) and 0.2% HA (Idroflog®, I/HA), with respect to changes on signs and inflammatory markers in a mouse DED model. Methods: Thirty 12-week-old C57BL/6 mice were exposed in a controlled-environment chamber as a desiccating stress model of DED for 35 days. At day 14 (T1), administration of 5 µL T or I in the right eye (RE) or NaCl 0.9% in the left eye (LE) started, twice a day. Animals were sacrificed after 7 (T2), 14 (T3), 21 (T4, endpoint) days from the beginning of treatment. Corneal fluorescein staining ratio (Image J), histological and histochemical assessment of ocular surface tissues (goblet cell GC density and characterization —PAS, Alcian blue pH 2.5, pH 1.0, and MUC4 expression—in the superior and inferior conjunctiva), and levels of inflammatory markers HLA-DR, IL-1β and TNF-α in cornea and conjunctiva were measured. Results: No animal fully recovered from DED signs at the endpoint. Difference between arms was observed at T3 and T4, with T treated eyes showing a higher corneal damage reduction, PAS-positive GC recovery, lower inflammatory marker expression as compared to the I treated ones. Conclusions: Data suggest that 21 days of treatment with T/HA improved signs, GC recovery and inflammatory markers in a DED mouse model, to a greater extent as compared to I/HA. Data suggest that 21 days of treatment with T/HA improved signs, GC recovery and inflammatory markers in a DED mouse model, to a greater extent as compared to I/HA.
Prevalence and Rate of Resolution of Left Atrial Thrombus in Patients with Non-Valvular Atrial Fibrillation: A Two-Center Retrospective Real-World Study
Authors: Faggiano Pompilio , Dinatolo Elisabetta , Moreo Antonella , De Chiara Benedetta , Sbolli Marco , Musca Francesco , Curnis Antonio , Belli Oriana , Giannattasio Cristina , Tomasi Cesare , Metra Marco , Santangelo Gloria
Published: March 2022

Background and aim. Thromboembolic events due to left atrial appendage (LAA) thrombosis are the main complication of non-valvular atrial fibrillation (NVAF). Although anticoagulants are effective in patients with NVAF, a minimal residual thromboembolic risk persists. Little is known about the prevalence of LAA thrombus and the rate of resolution after the recommended period of anticoagulation therapy, including vitamin K antagonists (VKA), heparin, and non-vitamin K antagonist oral anticoagulants (NOACs). Methods and results. We aimed to study the prevalence of LAA thrombus in an unselected cohort of patients undergoing transesophageal echocardiogram (TEE), and the determinants of LAA thrombus resolution. We retrospectively analyzed 8888 consecutive TEEs performed over five years in two high-volume centers and included all patients with LAA thrombus. A total of 265 patients (3%) had an LAA thrombus. Among these, 97% presented with AF. Fifty-eight percent of patients were on anticoagulants at least three weeks before the diagnosis. After the LAA thrombus diagnosis, VKAs were prescribed in 52%, heparin in 18.5%, and NOAC in 27% of patients. Among the 183 patients with repeat TEE, performed at (25–75th) 39 days (21–84), 67% showed resolution of the LAA thrombus. Although the rate of thrombus resolution was higher in patients treated with NOACs (NOACs 71%, VKA 66%, Heparin 60%) the difference between anticoagulants was statistically non-significant (VKA, OR 0.9, p = 0.83; NOAC, OR 1.23, p = 0.42; heparin, OR 0.69, p = 0.35). Thus, NOACs were demonstrated to be at least as effective as other anticoagulants in the rate of LAA thrombus resolution. Upon multivariate-adjusted analysis, higher LAA emptying velocities were the only predictor of thrombus resolution. In conclusion, the majority of patients were already on anticoagulants. NOACs could be at least as effective as other anticoagulants, yielding an LAA thrombus resolution in two-thirds of patients. This may have clinical relevance, especially in patients undergoing cardioversion or catheter ablation.
The Nephrologist’s Role in the Collaborative Multi-Specialist Network Taking Care of Patients with Diabetes on Maintenance Hemodialysis: An Overview
Authors: Cavallari Giuseppe , Mancini Elena
Published: March 2022

Diabetes mellitus is the leading cause of renal failure in incident dialysis patients in several countries around the world. The quality of life for patients with diabetes in maintenance hemodialysis (HD) treatment is in general poor due to disease complications. Nephrologists have to cope with all these problems because of the “total care model” and strive to improve their patients’ outcome. In this review, an updated overview of the aspects the nephrologist must face in the management of these patients is reported. The conventional marker of glycemic control, hemoglobin A1c (HbA1c), is unreliable. HD itself may be responsible for dangerous hypoglycemic events. New methods of glucose control could be used even during dialysis, such as a continuous glucose monitoring (CGM) device. The pharmacological control of diabetes is another complex topic. Because of the risk of hypoglycemia, insulin and other medications used to treat diabetes may need dose adjustment. The new class of antidiabetic drugs dipeptidyl peptidase 4 (DPP-4) inhibitors can safely be used in non-insulin-dependent end-stage renal disease (ESRD) patients. Nephrologists should take care to improve the hemodynamic tolerance to HD treatment, frequently compromised by the high level of ultrafiltration needed to counter high interdialytic weight gain. Kidney and pancreas transplantation, in selected patients with diabetes, is the best therapy and is the only approach able to free patients from both dialysis and insulin therapy.
Double Balloon Catheter (Plus Oxytocin) versus Dinoprostone Vaginal Insert for Term Rupture of Membranes: A Randomized Controlled Trial (RUBAPRO)
Authors: Devillard Eric , Petillon Fanny , Rouzaire Marion , Pereira Bruno , Accoceberry Marie , Houlle Céline , Dejou-Bouillet Lydie , Bouchet Pamela , Delabaere Amélie , Gallot Denis
Published: March 2022

Background: The aim of this study is to demonstrate that a double balloon catheter combined with oxytocin decreases time between induction of labor and delivery (TID) as compared to a vaginal dinoprostone insert in cases of premature rupture of membranes at term. Methods: This is a prospective, randomized, controlled trial including patient undergoing labor induction for PROM at term with an unfavorable cervix in Clermont-Ferrand university hospital. We compared the double balloon catheter over a period of 12 h with adjunction of oxytocin 6 h after catheter insertion versus dinoprostone vaginal insert. After device ablation, cervical ripening continued only with oxytocin. The main outcome was TID. Secondary outcomes concerned delivery mode, as well as maternal and fetal outcome, and were adjusted for parity. Results: 40 patients per group were randomized. Each group had similar baseline characteristics. The study failed to demonstrate reduced TID (16.2 versus 20.2 h, ES = 0.16 (−0.27 to 0.60), p = 0.12) in the catheter group versus dinoprostone except in nulliparous women (17.0 versus 26.5 h, ES = 0.62 (0.10 to 1.14), p = 0.006). The rate of vaginal delivery <24 h significantly increased with combined induction (88.5% versus 66.6%, p = 0.03). No statistical difference was observed concerning caesarean rate (12.5% versus 17.5%, p > 0.05), chorioamnionitis (0% versus 2.5%, p = 1), postpartum endometritis, or maternal or neonatal outcomes. Procedure-related pain and tolerance to devices were found to be similar for the two methods. Interpretation: The double balloon catheter combined with oxytocin is an alternative for cervical ripening in case of PROM at term, and may reduce TID in nulliparous women.
Statins and Colorectal Cancer Risk: A Population-Based Case-Control Study and Synthesis of the Epidemiological Evidence
Authors: Rodríguez-Miguel Antonio , Fernández-Antón Encarnación , Barreira-Hernández Diana , García-Rodríguez Luis A. , Gil Miguel , García-Lledó Alberto , De Abajo Francisco J.
Published: March 2022

(1) Background: The pleiotropic effects of statins may explain a chemoprotective action against colorectal cancer (CRC). Many studies have tested this hypothesis, but results have been inconsistent so far. Moreover, few have examined statins individually which is important for determining whether there is a class effect and if lipophilicity and intensity may play a role. (2) Methods: From 2001–2014, we carried out a study comprised of 15,491 incident CRC cases and 60,000 matched controls extracted from the primary healthcare database BIFAP. We fit a logistic regression model to compute the adjusted-odds ratios (AOR) with their 95% confidence intervals (CIs). Additionally, we carried out a systematic review and meta-analysis. (3) Results: Current use of statins showed a reduced risk of CRC (AOR = 0.87; 95% CI: 0.83–0.91) not sustained after discontinuation. The association was time-dependent, starting early (AOR6months–1year = 0.85; 95% CI: 0.76–0.96) but weakened beyond 3-years. A class effect was suggested, although only significant for simvastatin and rosuvastatin. The risk reduction was more marked among individuals aged 70 or younger, and among moderate-high intensity users. Forty-eight studies were included in the meta-analysis (pooled-effect-size = 0.90; 95% CI: 0.86–0.93). (4) Conclusions: Results from the case-control study and the pooled evidence support a moderate chemoprotective effect of statins on CRC risk, modified by duration, intensity, and age.
Comparison of Ultra-Mini Percutaneous Nephrolithotomy and Retrograde Intrarenal Surgery for Renal Stones: A Systematic Review and Meta-Analysis from the KSER Update Series
Authors: Jung Hae Do , Chung Doo Yong , Kim Do Kyung , Lee Min Ho , Lee Sin Woo , Paick Sunghyun , Jeon Seung Hyun , Lee Joo Yong ,   on behalf of the Korean Society of Endourology and Robotics (KSER) Research Committee
Published: March 2022

Miniaturized percutaneous nephrolithotomy (PCNL) and retrograde intrarenal surgery (RIRS) for renal stones have been developed to overcome the invasive disadvantages of PCNL. We aimed to compare the therapeutic effect and safety of ultra-mini percutaneous nephrolithotomy (UMPCNL) and RIRS for renal stones using an updated systematic review and meta-analysis. We searched clinical trials comparing UMPCNL and RIRS for renal stones using the PubMed, EMBASE, Cochrane Library, and Google Scholar databases up to October 2021. Seven studies were included in the current study. The renal stone size was 10–20 mm in three studies, 10–25 mm in one study, 10–35 mm in two studies, and not specified in one study. The stone-free rate of UMPCNL was higher than that of RIRS (p = 0.02; odds ratio (OR) = 2.01; 95% confidence interval (CI) = 1.12, 3.61). The complication rate showed no significant difference between UMPCNL and RIRS (p = 0.48; OR = 1.20; 95% CI = 0.73, 1.98). Regarding the operative time, UMPCNL was shorter than RIRS (p = 0.005; weighted mean difference (WMD) = −15.63; 95% CI = −26.60, −4.67). The hospital stay of UMPCNL was longer than that of RIRS (p = 0.0004; WMD = 1.48; 95% CI = 0.66, 2.31). UMPCNL showed higher efficacy than RIRS and similar safety to RIRS. UMPCNL may be a useful therapeutic option for moderate-sized renal stones.
Preoperative Assessment of Skeletal Muscle Mass and Muscle Quality Using Computed Tomography: Incidence of Sarcopenia in Patients with Intrahepatic Cholangiocarcinoma Selected for Liver Resection
Authors: Ardito Francesco , Coppola Alessandro , Rinninella Emanuele , Razionale Francesco , Pulcini Gabriele , Carano Davide , Cintoni Marco , Mele Maria Cristina , Barbaro Brunella , Giuliante Felice
Published: March 2022

Background: Sarcopenia is considered a predictor of poor postoperative and long-term results following liver resection for intrahepatic cholangiocarcinoma (ICC). The aim of our study was to assess the incidence of sarcopenia in patients resected for ICC and its relation to preoperative clinical factors. Methods: Patients resected for ICC in our unit, with available preoperative CT scans within one month before operation, were enrolled in the study. Skeletal muscle index (SMI) and skeletal muscle radiodensity (SMD) were assessed for each patient. Results: Thirty patients matched all inclusion criteria. Low SMI values were documented in 15 patients (50.0%), and low SMD values were documented in 10 patients (33.3%). SMI was significantly greater in males (p < 0.001). In patients who were underweight, the incidence of low SMI was significantly higher than that of high SMI (p = 0.031). In patients who were overweight/obese, the incidence of high SMI was significantly higher than that of low SMI (p = 0.003) and the incidence of low SMD was significantly higher than that of high SMD (p = 0.038). In the univariate analysis, no preoperative factors (clinical and tumor-related factors), in particular BMI, were found to be independent predictors of low SMI. Conclusions: The incidence of sarcopenia was 50.0% in patients selected for liver resection for ICC and was not related to the preoperative clinical factors. A multidisciplinary evaluation of the nutritional status is fundamental before liver resection in patients.
Effects of Floating Airbag on Cable Hydrodynamic Behaviors: An Experimental Study
Authors: Bao Shanjun , Zhang Zhigang , Yuan Zhoulong , Wang Jiancheng , Yu Qianhao , Liu Zhen
Published: March 2022

The development of an offshore island presented a need to construct an electricity grid with the laying of submarine cable. The floating airbag is a useful tool for cable construction, especially during landing. The effects of these airbags on cable hydrodynamic behaviors were investigated in this study. Regular wave conditions with various airbag intervals and cable masses were employed in wave flume tests. The vertical displacements and tensions of the cable under different test conditions were investigated. It was found that the peak values of the displacement amplitudes and tensions were obtained during the cable landing phase.
Arbuscular Mycorrhizal Fungi and Endophytic Fungi Activate Leaf Antioxidant Defense System of Lane Late Navel Orange
Authors: Li Qiu-Shuang , Xie Ya-Chao , Rahman Mohammed Mahabubur , Hashem Abeer , Abd_Allah Elsayed Fathi , Wu Qiang-Sheng
Published: March 2022

Arbuscular mycorrhizal (AM) fungi and endophytic fungi collectively symbiose well with plants and, thus, stimulate plant growth; however, it is not clear whether field inoculation of the fungi enhances the resistance potential of plants, particularly in citrus. In the present study, we inoculated AM fungi (Acaulospora scrobiculata, Diversispora spurca, and D. versiformis) and endophytic fungi (Piriformospora indica) on an eight-year-old lane late navel orange (Citrus sinensis (L.) Osb) trees grafted on Poncirus trifoliata in a field, and we analyzed the response of the leaf antioxidant defense system. Approximately 2 years after inoculation, the root fungal colonization rate and soil hyphal length significantly increased. Fungal inoculation significantly increased the activity of leaf antioxidant enzymes, such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, and the content of non-enzymatic antioxidants, such as reduced ascorbic acid and reduced glutathione. As a result, fungi-inoculated plants maintained lower concentrations of hydrogen peroxide and superoxide anion radicals and lower levels of membrane lipid peroxidation (according to malondialdehyde level) in leaves than uninoculated plants. Among them, inoculation of D. spurca and A. scrobiculata showed relatively higher effects in enhancing the antioxidant defense system than the other fungi. Furthermore, inoculation of D. spurca induced expressions of CsFe-SOD, CsMn-SOD, CsPOD, CsCAT1, and CsPRR7; inoculation of A. scrobiculata and D. versiformis induced expressions of CsCAT1; CsCAT1 and CsPOD were also induced by inoculation of P. indica. All four inoculations almost upregulated expressions of CsFAD6. AM fungi had superior effects than endophytic fungi (e.g., P. indica). According to our findings, inoculation with beneficial fungi, specifically mycorrhizal fungus D. spurca, activated the antioxidant defense system of field citrus trees, thus, having potentially superior resistance in inoculated plants.
Are Risk Factors for Postoperative Significant Hemorrhage following Total Knee Arthroplasty Potentially Modifiable? A Retrospective Cohort Study
Authors: Wu En-Bo , Hung Kuo-Chuan , Juang Sin-Ei , Chin Jo-Chi , Lu Hsiao-Feng , Ko Jih-Yang
Published: March 2022

Total knee arthroplasty (TKA) is the treatment of choice for end-stage osteoarthritis (OA) of the knee, because it alleviates pain and restores function of the knee. However, TKA-associated hemorrhage and subsequent anemia remain a concern. Most previous studies have defined meaningful postoperative bleeding as blood loss > 500 mL or hemoglobin (Hb) drop > 20 g/L. Therefore, we defined significant hemorrhage as a postoperative Hb drop more than 20 g/L in this study, and we investigated possible risk factors related to significant hemorrhage in TKA and whether these risk factors are modifiable. This retrospective study was conducted through a comprehensive review of the perioperative records of patients with OA of the knee who underwent TKA between January 2009 and December 2015 at our hospital. Patients were allocated into two groups: patients in Group A had their Hb drop ≤ 20 g/L; patients in Group B had their Hb drop > 20 g/L. Factors analyzed included sex, age, body mass index (BMI), the American Society of Anesthesiologists (ASA) classification, comorbidities, preoperative platelet count, use of tranexamic acid (TXA), operation time, and type of anesthesia. A total of 3350 patients met the criteria for analysis, with 1782 patients allocated to Group A and 1568 patients to Group B. Five independent risk factors for significant hemorrhage were identified: male sex (odds ratio(OR), 1.29; 95% confidence interval(CI), 1.08–1.53; p = 0.005), age (OR, 1.02; 95% CI, 1.01–1.03; p = 0.001), use of TXA (OR, 0.39; 95% CI, 0.34–0.45; p < 0.001), spinal anesthesia versus general anesthesia (OR, 0.71; 95% CI, 0.56–0.90; p = 0.004), and preoperative platelet count (OR, 0.96; 95% CI, 0.93–0.98; p = 0.001). Of these identified risk factors, preoperative platelet count, use of TXA, and spinal anesthesia are modifiable. These potentially modifiable risk factors need to be taken into consideration when making both the perioperative care and anesthesia plan by surgeons and anesthesiologists, especially in patients at risk of significant hemorrhage.
Machine Learning Model-Based Simple Clinical Information to Predict Decreased Left Atrial Appendage Flow Velocity
Authors: Li Chao , Dou Guanhua , Ding Yipu , Xin Ran , Wang Jing , Guo Jun , Chen Yundai , Yang Junjie
Published: March 2022

Background: Transesophageal echocardiography (TEE) is the first technique of choice for evaluating the left atrial appendage flow velocity (LAAV) in clinical practice, which may cause some complications. Therefore, clinicians require a simple applicable method to screen patients with decreased LAAV. Therefore, we investigated the feasibility and accuracy of a machine learning (ML) model to predict LAAV. Method: The analysis included patients with atrial fibrillation who visited the general hospital of PLA and underwent transesophageal echocardiography (TEE) between January 2017 and December 2020. Three machine learning algorithms were used to predict LAAV. The area under the receiver operating characteristic curve (AUC) was measured to evaluate diagnostic accuracy. Results: Of the 1039 subjects, 125 patients (12%) were determined as having decreased LAAV (LAAV < 25 cm/s). Patients with decreased LAAV were fatter and showed a higher prevalence of persistent AF, heart failure, hypertension, diabetes and stroke, and the decreased LAAV group had a larger left atrium diameter and a higher serum level of NT-pro BNP than the control group (p < 0.05). Three machine-learning models (SVM model, RF model, and KNN model) were developed to predict LAAV. In the test data, the RF model performs best (R = 0.608, AUC = 0.89) among the three models. A fivefold cross-validation scheme further verified the predictive ability of the RF model. In the RF model, NT-proBNP was the factor with the strongest impact. Conclusions: A machine learning model (Random Forest model)-based simple clinical information showed good performance in predicting LAAV. The tool for the screening of decreased LAAV patients may be very helpful in the risk classification of patients with a high risk of LAA thrombosis.
Goat Breeding: A Possible Sustainable Way to Manage Xerophytic Thickets in Southwestern Madagascar
Authors: Randriamalala Josoa R. , Hervé Dominique
Published: March 2022

Spiny thickets or xerophytic thickets (XTs) are a type of shrubby vegetation found in the far south and southwest of Madagascar, the driest parts of the island. This type of vegetation, which is rich in endemic animal and plant species, is endangered. Extensive local goat breeding (Capra hircus, for meat and milk production) based on XT browsing is an important source of household income. The aim of this paper is to analyse the possibility of using improved goat breeding as an alternative to wood charcoal (WC) production and slash-and-burn agriculture (SBA), which are unsustainable activities. The literature on (i) the impacts of SBA, WC production, and goat browsing on the XT ecosystem and (ii) the income provided by these three activities is reviewed to determine the sustainability level of improved goat husbandry. SBA and WC production reduced XT biomass and XT cover area, while goat browsing alone, at a stocking rate of one head per hectare, did not affect XT leaf biomass production and shrub regeneration. Furthermore, batch breeding and flushing techniques provided the highest annual income, mainly from the sale of surplus animal products. This improved goat husbandry may be a sustainable alternative to WC production. However, increasing the current stocking rate is necessary to surpass the combined income of WC production and SBA. An estimation of the XT carrying capacity would offer a basis to assess whether this ecosystem would support a higher stocking rate.
Silicon Nitride-Based Micro-Apertures Coated with Parylene for the Investigation of Pore Proteins Fused in Free-Standing Lipid Bilayers
Authors: Ahmed Tanzir , Bafna Jayesh Arun , Hemmler Roland , Gall Karsten , Wagner Richard , Winterhalter Mathias , Vellekoop Michael J. , van den Driesche Sander
Published: March 2022

In this work, we present a microsystem setup for performing sensitive biological membrane translocation measurements. Thin free-standing synthetic bilayer lipid membranes (BLM) were constructed in microfabricated silicon nitride apertures (<100 µm in diameter), conformal coated with Parylene (Parylene-C or Parylene-AF4). Within these BLMs, electrophysiological measurements were conducted to monitor the behavior of different pore proteins. Two approaches to integrate pore-forming proteins into the membrane were applied: direct reconstitution and reconstitution via outer membrane vesicles (OMVs) released from Gram-negative bacteria. The advantage of utilizing OMVs is that the pore proteins remain in their native lipid and lipopolysaccharide (LPS) environment, representing a more natural state compared to the usage of fused purified pore proteins. Multiple aperture chips can be easily assembled in the 3d-printed holder to conduct parallel membrane transport investigations. Moreover, well defined microfabricated apertures are achievable with very high reproducibility. The presented microsystem allows the investigation of fast gating events (down to 1 ms), pore blocking by an antibiotic, and gating events of small pores (amplitude of approx. 3 pA).
Hot Compression Behavior of a Novel High Entropy Alloy
Authors: Mei Jinna , Wu Tiandong , Wei Na , Xue Fei , Cai Zhen , Xue Xiangyi
Published: March 2022

In this study, the flow behavior of a novel high entropy alloy, (FeNi)67Cr15Mn10Al5Ti3, was investigated through isothermal compression performed at temperature range of 980–1100 °C using strain rates of 0.01 s−1 and 0.1 s−1. The alloy was mainly composed of a face-centered cubic (FCC) phase and a small amount of body-centered cubic (BCC) phase. During deformation, the alloy exhibited typical single-peak type flow curve at all testing conditions. The stress exponent was determined to be ~5.5 with an apparent activation energy of ~426 kJ/mol, which indicated that dislocation creep was the rate-controlling process. Metallurgical inspection revealed that due to the plastic incompatibility of the two phases, the deformation is non-uniform especially at lower temperature and higher strain rate. Continuous dynamic recrystallization (CDRX) intensively occurred in both phases, but the recrystallization seemed to be much easier in the BCC phase. Dense low angle grain boundaries (LAGBs) were produced as a consequence of CDRX. At a lower strain rate, the LAGB ratio was evidently decreased with the increasing temperature. The sub-grain size was sensitive to the deformation parameter especially at a high temperature and low strain rate. A low temperature and high strain rate were beneficial for grain refinement but a much higher straining was required.
Metal–Organic-Framework-Derived Ball-Flower-like Porous Co3O4/Fe2O3 Heterostructure with Enhanced Visible-Light-Driven Photocatalytic Activity
Authors: Cao Qi , Li Qingqing , Pi Zhichao , Zhang Jing , Sun Li-Wei , Xu Junzhou , Cao Yunyi , Cheng Junye , Bian Ye
Published: March 2022

A porous ball-flower-like Co3O4/Fe2O3 heterostructural photocatalyst was synthesized via a facile metal–organic-framework-templated method, and showed an excellent degradation performance in the model molecule rhodamine B under visible light irradiation. This enhanced photocatalytic activity can be attributed to abundant photo-generated holes and hydroxyl radicals, and the combined effects involving a porous structure, strong visible-light absorption, and improved interfacial charge separation. It is notable that the ecotoxicity of the treated reaction solution was also evaluated, confirming that an as-synthesized Co3O4/Fe2O3 catalyst could afford the sunlight-driven long-term recyclable degradation of dye-contaminated wastewater into non-toxic and colorless wastewater.
Electrospinning Synthesis of Na0.5Bi0.5TiO3 Nanofibers for Dielectric Capacitors in Energy Storage Application
Authors: Liu Yuan , Luo Hang , Gao Zhe , Xie Haoran , Guo Ru , Wang Fan , Zhou Xuefan , Cao Jun , Zhang Dou
Published: March 2022

Dielectric composites based on ferroelectric ceramics nanofibers are attracting increasing attention in capacitor application. In this work, the sol–gel method and electrospinning technology are utilized to prepare one-dimensional Na0.5Bi0.5TiO3 (NBT) nanofibers, and the influence of electrospinning process parameters such as spinning voltage, liquid supply rate, and collector speed on the morphology and structure of nanofibers are systematically explored. The final optimized parameters include the applied voltage of 20 kV, the solution flow rate of 1 mL/h, and the collector’s rotation speed of 1500 rpm. The optimized NBT nanofibers are introduced into the PVDF polymer matrix for energy storage application. Owing to the enhanced interfacial polarization between PVDF matrix and NBT nanofibers with a high aspect ratio, the NBT–PVDF nanocomposites achieve a high discharge energy density of 14.59 J cm−3 and an energy efficiency of 53.69% at 490 kV mm−1, which are higher than those of pure PVDF, i.e., 10.26 J cm−3 and 48.17% at 420 kV mm−1, respectively. The results demonstrate that the strategy of synthesizing NBT nanofibers using the electrospinning method is of great potential for high-performance dielectric capacitor application.
Morphological, Behavioral, and Molecular Characterization of Avian Schistosomes (Digenea: Schistosomatidae) in the Native Snail Chilina dombeyana (Chilinidae) from Southern Chile
Authors: Oyarzún-Ruiz Pablo , Thomas Richard , Santodomingo Adriana , Collado Gonzalo , Muñoz Pamela , Moreno Lucila
Published: March 2022

Avian schistosomes are blood flukes parasitizing aquatic birds and snails, which are responsible for a zoonotic disease known as cercarial dermatitis, a hypersensitive reaction associated to the cutaneous penetration of furcocercariae. Despite its worldwide distribution, its knowledge is fragmentary in the Neotropics, with most of data coming from Argentina and Brazil. In Chile, there are only two mentions of these parasites from birds, and one human outbreak was associated to the genus “Trichobilharzia”. However, the identity of such parasites is pending. The aim of this study was to identify the furcocercariae of avian schistosomes from Southern Chile using an integrative approach. Thus, a total of 2283 freshwater snails from different families were collected from three different regions. All snails were stimulated for the shedding of furcocercariae, but only Chilina dombeyana (Chilinidae) from the Biobío region was found to be parasitized. The morphology and phylogenetic analyses of 28S and COI genes stated two lineages, different from Trichobilharzia, shared with Argentina. This study provides new information on Neotropical schistosomes, highlighting the need for major research on these neglected trematodes, which are considered to be emerging/re-emerging parasites in other parts of the globe as consequence of anthropogenic disturbances and climatic change. Highlights: 1. Two different lineages (Lineage I and II) were described and molecularly characterized (28S and COI genes); 2. Cercaria chilinae I y II are proposed as a synonymous of Lineage II. Thus, a total of four different lineages of avian schistosomes are related to Chilina spp.; 3. Chilina spp. represents an important intermediate host for avian schistosomes in South America, constituting a reservoir de schistosomes with zoonotic potential; 4. Coinfection between the two different lineages was found, a finding previously not reported for avian schistosomes; 5. Expansion in the geographic distribution of Nasusbilharzia melancorhypha from its original record in Argentina, with Chilina dombeyana as an additional intermediate host.
Diversity and Genetic Structure of Theileria annulata in Pakistan and Other Endemic Sites
Authors: Al-Hamidhi Salama , Parveen Asia , Iqbal Furhan , Asif Muhammad , Akhtar Naheed , Elshafie Elshafie I. , Beja-Pereira Albano , Babiker Hamza A.
Published: March 2022

Background: Theileria annulata is a tick-borne protozoan parasite responsible for bovine theileriosis, a disease that impacts cattle population in many developing countries. Development and deployment of effective control strategies, based on vaccine or therapy, should consider the extent of diversity of the parasite and its population structure in different endemic areas. In this study, we examined T. annulata in Pakistan and carried out a comparative analysis with similar data garneted in other areas, to provide further information on the level of parasite diversity and parasite genetic structure in different endemic areas. Methods: The present study examined a set of 10 microsatellites/minisatellites and analyzed the genetic structure of T. annulata in cattle breeds from Pakistan (Indian sub-continent) and compared these with those in Oman (Middle East), Tunisia (Africa), and Turkey (Europe). Result: A high level of genetic diversity was observed among T. annulata detected in cattle from Pakistan, comparable to that in Oman, Tunisia, and Turkey. The genotypes of T. annulata in these four countries form genetically distinct groups that are geographically sub-structured. The T. annulata population in Oman overlapped with that in the Indian Subcontinent (Pakistan) and that in Africa (Tunisia). Conclusions: The T. annulata parasite in Pakistan is highly diverse, and genetically differentiated. This pattern accords well and complements that seen among T. annulata representing the global endemic site. The parasite population in the Arabian Peninsula overlapped with that in the Indian-Subcontinent (India) and that in Africa (Tunisia), which shared some genotypes with that in the Near East and Europe (Turkey). This suggests some level of parasite gene flow, indicative of limited movement between neighboring countries.
Development, Therapeutic Evaluation and Theranostic Applications of Cubosomes on Cancers: An Updated Review
Authors: Almoshari Yosif
Published: March 2022

Cancer is a group of disorders characterized by aberrant gene function and alterations in gene expression patterns. In 2020, it was anticipated that 19 million new cancer cases would be diagnosed globally, with around 10 million cancer deaths. Late diagnosis and interventions are the leading causes of cancer-related mortality. In addition, the absence of comprehensive cancer therapy adds to the burden. Many lyotropic non-lamellar liquid-crystalline-nanoparticle-mediated formulations have been developed in the last few decades, with promising results in drug delivery, therapeutics, and diagnostics. Cubosomes are nano-structured liquid-crystalline particles made of specific amphiphilic lipids in particular proportions. Their ability to encapsulate lipophilic, hydrophilic, and amphiphilic molecules within their structure makes them one of a kind. They are biocompatible, versatile drug carriers that can deliver medications through various routes of administration. Many preclinical studies on the use of cubosomes in cancer treatment and theranostic applications have been conducted. However, before cubosomes may be employed in clinical practice, significant technical advances must be accomplished. This review summarizes the development of cubosomes and their multifunctional role in cancer treatment based on the most recent reports.
Phosphorescence and Photophysical Parameters of Porphycene in Cryogenic Matrices
Authors: Golec Barbara , Gorski Aleksander , Waluk Jacek
Published: March 2022

Matrix isolation studies were carried out for porphycene, an isomer of porphyrin, embedded in solid nitrogen and xenon. The external heavy atom effect resulted in nearly a 100% population of the triplet state and in the appearance of phosphorescence, with the origin located at 10163 cm−1. This energy is much lower than that corresponding to the T1 position in porphyrin. This difference could be explained by postulating that the orbital origin corresponds in both isomers to the second excited singlet state, which lies much closer to S1 in porphycene. Most of the vibrational frequencies observed in the phosphorescence spectrum correspond to totally symmetric modes, but several ones were assigned to the out-of-plane Bg vibrations. These bands are not observed in fluorescence, which suggests their possible role in vibronic-spin-orbit coupling.
Infrared Ocean Image Simulation Algorithm Based on Pierson–Moskowitz Spectrum and Bidirectional Reflectance Distribution Function
Authors: Chen Xueqi , Zhou Lin , Zhou Meng , Shao Ajun , Ren Kan , Chen Qian , Gu Guohua , Wan Minjie
Published: March 2022

Infrared ocean image simulation has been widely used in water-pollution prevention, meteorological observation and melting-ice monitoring. However, in actual remote sensing observation scenes, the simulation images provided by conventional algorithms are lacking sufficient wave details because the viewing angle and the scale of simulation images are simplex. In this paper, an infrared ocean image simulation algorithm based on the Pierson–Moskowitz spectrum and a bidirectional reflectance distribution function is proposed. First, a 3D model of ocean surface is set up based on Pierson–Moskowitz spectrum. Then, the imaging position is calculated by the pinhole camera imaging method, which describes how each point of the 3D model is mapping to the 2D image. Next, by using a bidirectional reflectance distribution function, the radiation intensity from every point of the ocean model to the camera is computed. Finally, we figure up the sum of the radiation intensity received by every point of the detector and obtain the infrared simulation ocean image by quantizing the radiation intensity sum to grayscale. The entropy of the simulation images is 2.725, which is, respectively, improved by 71.86% and 16.83% compared with two other algorithms. The Kullback–Leibler divergence of the simulation images is 11.446, which is improved by 0.54% and 0.59% compared with other algorithms. The quantitative experimental results prove that the authenticity and clarity of the presented simulation images have remarkable advantages over conventional algorithms.
Demonstration of Performance Improvement in Multi-User NOMA VLC System Using Joint Transceiver Optimization
Authors: Wu Tong , Wang Zixiong , Han Shiying , Yu Jinlong , Jiang Yang
Published: March 2022

The bit error ratio (BER) performance of a non-orthogonal multiple access (NOMA) visible light communication (VLC) system is poor due to the unequal distances between adjacent points in the superposition constellation (SC). In this paper, we propose a novel scheme to improve the BER performance by adjusting parameters to change the shape of SC at the transmitter and by adjusting the parameters of successive interference cancellation (SIC) decoding at the receiver simultaneously, which is called a SC and SIC adjustment (SC-SIC-A) scheme. For multi-user NOMA VLC system, we derive the closed-form BER expression for each user, where the modulation format is four-quadrature amplitude modulation. According to the derived BER expressions, we formulate an optimization problem that minimizes the average BER for all users by adjusting the obtained parameters of SC and SIC decoding via differential evolution algorithm. The improvement of capacity performance is investigated consequently. In order to verify the feasibility and effectiveness of the proposed SC-SIC-A scheme, we carried out theoretical analysis, Monte Carlo simulation and experiments of two-user and three-user NOMA VLC systems. Results show that the SC-SIC-A scheme outperforms the existing schemes in NOMA VLC system, where the signal-to-noise ratio (SNR) reductions to achieve BER of 10−3 are 1.3 dB and 0.8 dB for both users in the two-user NOMA VLC system, respectively, and the SNR reductions to achieve BER of 10−3 are 5.7 dB, 4.3 dB and 4.6 dB for all users in the three-user NOMA VLC system, respectively.
Defoliation Change of European Beech (Fagus sylvatica L.) Depends on Previous Year Drought
Authors: Ognjenović Mladen , Seletković Ivan , Potočić Nenad , Marušić Mia , Tadić Melita Perčec , Jonard Mathieu , Rautio Pasi , Timmermann Volkmar , Lovreškov Lucija , Ugarković Damir
Published: March 2022

European beech (Fagus sylvatica L.) forests provide multiple essential ecosystem goods and services. The projected climatic conditions for the current century will significantly affect the vitality of European beech. The expected impact of climate change on forest ecosystems will be potentially stronger in southeast Europe than on the rest of the continent. Therefore, our aim was to use the long-term monitoring data of crown vitality indicators in Croatia to identify long-term trends, and to investigate the influence of current and previous year climate conditions and available site factors using defoliation (DEF) and defoliation change (ΔDEF) as response variables. The results reveal an increasing trend of DEF during the study period from 1996 to 2017. In contrast, no significant trend in annual ΔDEF was observed. The applied linear mixed effects models indicate a very strong influence of previous year drought on ΔDEF, while climate conditions have a weak or insignificant effect on DEF. The results suggest that site factors explain 25 to 30% DEF variance, while similar values of conditional and marginal R2 show a uniform influence of drought on ΔDEF. These results suggest that DEF represents the accumulated impact of location-specific stressful environmental conditions on tree vitality, while ΔDEF reflects intense stress and represents the current or recent status of tree vitality that could be more appropriate for analysing the effect of climate conditions on forest trees.
The Effect of Phosphate on the Activity and Sensitivity of Nutritropism toward Ammonium in Rice Roots
Authors: Yamazaki Kiyoshi , Fujiwara Toru
Published: March 2022

Understanding how plants determine growth direction from environmental cues is important to reveal optimal strategies in plant survival. Nutritropism is the directional growth of plant roots towards nutrient sources. Our previous study showed that an NH4+ gradient stimulates nutritropism in the lateral roots, but not in the main roots, of a rice cultivar. In the present study, we report nutritropism in the main roots of rice accessions among the World Rice Core Collection, including WRC 25. We investigated the effects of components in nutrient sources on nutritropism in WRC 25. Nutritropism in main roots was stimulated by NH4+ and significantly enhanced by Pi. We found that roots required more NH4+ stimulation for nutritropic responses in the presence of higher Pi, meaning that Pi desensitized root nutritropism. These results indicate that Pi acts as an activator and a desensitizer in nutritropism. Such a regulation of nutritropism would be important for plants to decide their optimum growth directions towards nutrient sources, gravity, moisture, or other stimuli.
Ecophysiological and Growth-Related Traits of Two Geophytes Three Years after the Fire Event in Grassland Steppe
Authors: Popović Zorica , Vidaković Vera
Published: March 2022

Deliblato Sands is the single largest expanse of sand in Europe. It is the most fire-prone area of Serbia due to the absence of surface water, sandy soils, specific microclimate conditions, and vegetation composition. Post-fire regeneration is a long-term process that includes many aspects of vegetation regrowth and habitat recovery. In the third year following one of the disastrous fires, the growth dynamics of two geophyte species in unburned and burned sites were studied. During the growing season, burned and unburned populations of Crocus reticulatus Steven ex Adam and Iris pumila L. were assessed for growth parameters (biomass production, specific leaf area, leaf area index) and leaf-level ecophysiological traits (photosystem II efficiency, chlorophyll amount, relative water content). Species acclimated differently to changed abiotic and biotic site conditions after the fire event. C. reticulatus burned and unburned populations differed significantly in terms of flowering phenology and ecophysiological traits, whereas I. pumila burned and unburned populations differed significantly in terms of growth parameters. The findings support the assertion that geophytes are generally well adapted to environmental disturbances. Species, however, responded differently to fire-induced changes in the physicochemical and biotic environment, depending on their ecological requirements and adaptive capacity.
Experimental Studies on the Influence of Plasma Treatment of Polyethylene in Carbon Fiber Composites: Mechanical and Morphological Studies
Authors: K R Sumesh , Anton Jakub , Spatenka Petr , Sourkova Hana Jelinek
Published: March 2022

This research focused on enhancement of mechanical properties in carbon fiber (CF)-filler-reinforced linear low-density polyethylene (PE) matrix composites. A hand layup method using an oven was used as the fabrication method. Improvement in adhesion was achieved by oxygen plasma treatment to the PE matrix. CF and PE were initially mixed by normal stirring, ultrasonication and mechanical stirring before being filtered and dried for fabrication. Better tensile results were observed with a plasma-treated polyethylene (PEP)/10 wt.% CF combination, with a maximum tensile strength of 21.5 MPa and improvement in the properties of up to 12.57% compared to non-plasma PE with the same CF addition. The addition of carbon fibers at 13 and 15 wt.% resulted in a reduction in the tensile strength properties to 18.2 MPa and 17.7 MPa, respectively. This reduction in tensile strength was due to agglomeration of CF with plasma- and non-plasma-treated PE. The fabrication condition of 180 °C temperature for 20 min showed better tensile properties than other conditions. The SEM results following tensile testing revealed enhanced CF filler adherence with plasma PE results, as well as fewer surface deformations. A higher flexural strength of 25.87 MPa was observed for the plasma treated PE/7 wt.% CF combination.
Integrating Boronic Esters and Anthracene into Covalent Adaptable Networks toward Stimuli-Responsive Elastomers
Authors: Liu Zhiyong , Ma Youwei , Xiang Yixin , Shen Xianrong , Shi Zixing , Gao Jiangang
Published: March 2022

Stimuli-responsive polymer materials have a promising potential application in many areas. However, integrating multi-stimuli into one elastomer is still a challenge. Here, we utilized boronic esters and anthracene to prepare a cross-linked poly(styrene-butadiene-styrene) (SBS) which was endowed with responsiveness to three stimuli (light, heat, and alcohols). SBS was first functionalized with a certain amount of dihydroxyl groups via a thiol-ene “click” reaction between unsaturated double bonds in PB block and thioglycerol. Then, 9-anthraceneboronic acid was applied to form a cross-linked SBS network upon heat and ultraviolet radiation (λ = 365 nm). The prepared elastomer was demonstrated to be stimuli-responsive based on the dynamic nature of boronic esters and the reversible dimerization of anthracene. In addition, the mechanical properties of the elastomer could be regulated continuously owing to the stimulus responsiveness to ultraviolet or heat.
The Product Customization Process in Relation to Industry 4.0 and Digitalization
Authors: Pech Martin , Vrchota Jaroslav
Published: March 2022

Today’s customer no longer wants one-size-fits-all products but expects products and services to be as tailored as possible. Mass customization and personalization are becoming a trend in the digitalization strategy of enterprises and manufacturing in Industry 4.0. The purpose of the paper is to develop and validate a conceptual model for leveraging Industry 4.0 and digitalization to support product customization. We explored the implications and impacts of Industry 4.0 and digitalization on product customization processes and determine the importance of variables. We applied structural equation modeling (SEM) to test our hypotheses regarding the antecedents and consequences of digitalization and Industry 4.0. We estimated the process model using the partial least squares (PLS) method, and goodness of fit measures show acceptable values. The proposed model considers relationships between technology readiness, digitalization, internal and external integration, internal value chain, and customization. The results show the importance of digitalization and technology readiness for product customization. The results reveal that the variable of internal integration plays a crucial mediating role in applying new technologies and digitalization for customization. The paper’s main contribution is the conclusion that, for successful implementation of the customization process, models are required to focus on the internal and external factors of the business environment. Our findings are supported by various practical applications of possible product customization.