bluuebunny/crossref_metadata_2025 huggingface dataset:
Lime treatment of stabilized leachates
Authors: Renou S. , Poulain S. , Givaudan J. G. , Sahut C. , Moulin P.
Published: February 2009

Reverse Osmosis is the most widely used method for treating municipal solid waste landfill leachates, since it produces a permeate in compliance with reject requirements. However, the efficiency of this process at the industrial scale is limited mainly because of membrane fouling and the high osmotic pressures involved. Although lime precipitation is traditionally used to eliminate the temporary hardness of water by decarbonation, it has also been shown to be highly efficient in removing humic substances which are known to have strong fouling potential towards membranes. Our objective is to study the lime/leachate physico-chemistry, in order to determine the potential of the lime precipitation as pre-treatment for reverse osmosis. The results show that the lime treatment makes it possible (i) to act efficiently on the inorganic fraction of leachates through a decarbonation mechanism which entails massive precipitation of the carbonates under the form of CaCO3, (ii) to eliminate by co-precipitation the high Molecular Weight (MW) organic macromolecules (>50,000 g.mol−1) such as humic acids, and (iii) to generate a stable residue that can be easily stored at a landfill. The reverse osmosis step will be facilitated through significant reduction of the osmotic pressures and prevention of membrane fouling.
Photocatalytic water disinfection of Cryptosporidium parvum and Giardia lamblia using a fibrous ceramic TiO2 photocatalyst
Authors: Navalon Sergio , Alvaro Mercedes , Garcia Hermenegildo , Escrig Daniel , Costa Víctor
Published: February 2009

Cryptosporidium parvum and Giardia lamblia are two of the most chlorine resistant microorganisms with notable adverse effects on humans. Our study shows that waters containing these two protozoa at low concentrations can be efficiently disinfected in continuous flow by using a commercial fibrous ceramic TiO2 photocatalyst. The efficiency of the photocatalytic disinfection is largely enhanced by adding a small concentration of chlorine. In this way, the residence time on the photoreactor can be considerably shortened. In contrast, under the same conditions and radiance power, UV light without any photocatalyst is significantly less efficient, particularly for G. lamblia. These results exemplify the advantages of the photocatalytic process for safe and complete water disinfection.
Biological degradation of a mixture of municipal wastewater and organic garbage leachate in expanded bed anaerobic reactors and a zeolite filter
Authors: Castilla P. , Aguilar L. , Escamilla M. , Silva B. , Milán Z. , Monroy O. , Meraz M.
Published: February 2009

Municipal wastewater was amended with organic garbage leachates at a concentration around 700 mgCODsoluble/L and fed to three different anaerobic systems to compare their performance: a down flow fluidized bed (DFFB), an expanded granular sludge bed (EGSB) and a zeolite-packed anaerobic filter reactor (ZPF). The DFFB and EGSB reactors were operated at HRT of 6 and 4 h and the ZPF reactor at 12 and 36 h. Organic loads rate for the DFFB reactor were 2.3±0.9 and 4.8±1.8 gCOD/L·d, with removal efficiencies around 40% and a methane productivity of 0.2±0.03 L/Lreactor·d. For the EGSB reactor, organic loads tested were 2.1±0.9 and 4.3±1.3 gCOD/L·d, removal efficiencies attained were of 77.6±12.7% and 84.4±4.9%, respectively at both conditions and total suspended solids were removed in 54.6±19.3%, while methane productivity at 4 h HRT was of 1.29±0.4 L/Lreactor·d. The ZPF reactor was operated at lower organic loading rates, 1.4±0.27 and 0.42±0.13 gCOD/L·d and attained removal efficiencies of 48±18% and 83±8%, respectively, reaching a methane productivity of 0.21±0.09 and 0.12±0.04 L/Lreactor·d, 83±8.0% of total suspended solids were retained in the reactor and as HRT was increased ammonium concentrations increased in 39%. Specific methanogenic activity in all systems was around 0.2 gCOD-CH4/gVSS d.
Development of the modified activated sludge model describing nitrite inhibition of aerobic phosphate uptake
Authors: Yoshida Y. , Kim Y. , Saito T. , Tanaka K.
Published: February 2009

Since metabolic pathway and enzymatic clarification of poly-phosphate accumulating organisms (PAOs) are still unclear, biological phosphorus removal (BPR) sometimes become unstable. We have focused on nitrite as one of unknown factors deteriorating BPR performance. And we obtained some findings from previous studies, namely 1) nitrite inhibits phosphate uptake and growth of PAOs, 2) nitrite inhibition persists even after nitrite disappearance, 3) PAOs with the higher relative anoxic activity are less sensitive to nitrite exposure. This study provides the modified Activated Sludge Model No.2d (ASM2d) to express nitrite inhibition properly by incorporating the assumed intracellular reaction product (reaction complex) of nitrite. The model also considers the tolerance mechanism of nitrite inhibition by the denitrifying activity of PAOs. From the results of previous experiments and relevant references, the modified model called “Nitrite-Complex model” incorporates new components, namely nitrite (SNO2) and reaction complex (Xcomplex), to ASM2d. The model incorporation only nitrite inhibition was able to fit with measured phosphate concentration while nitrite exists. Whereas, this model couldn't describe the persistence of nitrite inhibition after nitrite disappeared. However, result of nitrite-complex model incorporating nitrite and reaction complex inhibition, it fits well with measured phosphate concentration not only before nitrite disappeared but also after nitrite disappeared.
Application of combined physicochemical and biological processes for enhanced treatment of avermectin fermentation wastewater
Authors: Yu Anfeng , Huang PengYu , Gui Dawei , Wang Haisheng , Feng Quan , Chu Libing , Xing Xin-Hui
Published: February 2009

This paper aimed at developing the enhanced biological treatment processes for treating avermectin fermentation wastewater (AFW). After UASB treatment and chemical coagulation, the pretreated AFW was subsequently flowed into a rCAA reactor (reactor with repeated coupling of aerobes and anaerobes using macroporous carriers) system for further pollutant degradation and excess sludge reduction. By the treatment with chemical coagulation, COD, total nitrogen and total phosphorus concentration of treated AFW were eliminated to 550–700 mg/L, 130–160 mg/L and 1 mg/L, respectively, and the dark color of the wastewater was greatly bleached. After this decolorized wastewater was treated by the following rCAA bioreactor, the COD could be reduced to around 200–300 mg/L, while the further decrease of COD less than 200 mg/L was difficult. The Biolog analysis and OUR test for the water treated by rCAA bioreactor demonstrated that the effluent from chemical coagulation contained some unknown compounds with low biodegradability and would simplify the microbial community in the subsequent rCAA reactor.
Identification of inhibitory effects of industrial effluents on nitrification
Authors: Žgajnar Gotvajn A. , Zagorc-Končan J.
Published: February 2009

The aim of our work was to determine the extent of inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation (ISO 8192 2007) for various wastewaters. We have selected several types of wastewaters different in their origin and composition: pharmaceutical wastewater, tannery wastewater and municipal landfill leachate. To confirm results of toxicity testing, additional ready biodegradability assessment test with measurement of oxygen consumption was accomplished to indicate the impact of effluents to nitrification process. Pharmaceutical wastewater was toxic to activated sludge, but it inhibited heterotrophic microorganisms much more than nitrifying ones. Biodegradability testing confirmed low impact to nitrification by high, non-suppressed oxygen consumption for nitrification process. Tannery effluent inhibited nitrification significantly (180 min EC50 was 57 vol.%), but it did not affect heterotrophic microorganisms. Landfill leachate was very toxic to heterotrophic microorganisms (180 min EC50 was 3 vol.%), while it inhibited nitrification less (180 min EC50 was 24 vol.%). Presented research confirmed that the investigated experimental method is a reliable one for detection of occurrence of substances inhibiting nitrification in different industrial effluents. With regular monitoring of inhibitory impact biological treatment process upsets could be avoided and treatment optimised.
Effect of speciation transformation on the coagulation behavior of Al13 and Al13 aggregates
Authors: Wu Xiaohong , Ye Changqing , Wang Dongsheng , Ge Xiaopeng , Tang Hongxiao
Published: February 2009

Flocculation of kaolin suspension with aluminium fractal polycations was investigated as a function of aluminium concentration and pH. Aluminium flocculants included Al13 and Al13 aggregates with OH/Al ratio of 2.6 and 2.8, respectively. The flocculation kinetics and floc size distribution were monitored by light scattering. The characterization of flocculants showed that the tridecatmer Al13 and bridged [Al13]n with out-sphere structure were the dominant species for all flocculants in a wide pH range. The coagulation results indicated that the pre and in situ-formed [Al13]n play a key role in removing particles. With the increasing concentration of [Al13]n, coagulation mechanisms were transformed from charge-neutralization, electro-patch coagulation to bridge-aggregation. Moreover, sweep-flocculation was involved at higher dosage besides other three mechanisms when amorphous aluminium oxides formed. Hence, chemical interaction between particles and flocculants evolved from surface adsorption to surface precipitation for aluminium polycations by virtue of species transformation.
Occurrence and elimination of antibiotics at four sewage treatment plants in Japan and their effects on bacterial ammonia oxidation
Authors: Ghosh Gopal Chandra , Okuda Takashi , Yamashita Naoyuki , Tanaka Hiroaki
Published: February 2009

The occurrence and elimination of seventeen antibiotics (three macrolides: azithromycin, clarithromycin and roxithromycin; five quinolones: ciprofloxacin, enrofloxacin, levofloxacin, nalidixic acid and norfloxacin; five sulfonamides: sulfadimethoxine, sulfadimizine, sulfamerazine, sulfamethoxazole and sulfamonomethoxine; and others: tetracycline, lincomycin, salinomycin and trimethoprim) were investigated at four full-scale sewage treatment plants in Japan. The highest concentration was recorded for clarithromycin (1,129 to 4,820 ng/L) in influent, followed by azithromycin (160 to 1,347 ng/L), levofloxacin (255 to 587 ng/L) and norfloxacin (155 to 486 ng/L). A vary inconsistence picture was obtained with negative to over 90% removal. Nalidixic acid (53 to100%) exhibited higher removal efficiency followed by norfloxacin (75 to 95%), levofloxacin (40 to 90%), ciprofloxacin (60 to 83%) and enrofloxacin (38 to 74%). Among macrolides, clarithromycin (50 to 88%) and azithromycin (34 to 86%) showed relatively higher removal efficiency than roxithromycin (−32 to 59%). For most of the antibiotics removal efficiency was higher in A2O and AO based secondary treatment process than CAS process. The effect of the antibiotics on bacterial ammonia oxidation determined by oxygen uptake rate presented that there was no significant effect below 0.05 mg/L of each antibiotics. Even at the same concentration, antibiotics in mixed condition had higher inhibition effects than individuals.
Wastewater treatment models in teaching and training: the mismatch between education and requirements for jobs
Authors: Hug Thomas , Benedetti Lorenzo , Hall Eric R. , Johnson Bruce R. , Morgenroth Eberhard , Nopens Ingmar , Rieger Leiv , Shaw Andrew , Vanrolleghem Peter A.
Published: February 2009

As mathematical modeling of wastewater treatment plants has become more common in research and consultancy, a mismatch between education and requirements for model-related jobs has developed. There seems to be a shortage of skilled people, both in terms of quantity and in quality. In order to address this problem, this paper provides a framework to outline different types of model-related jobs, assess the required skills for these jobs and characterize different types of education that modelers obtain “in school” as well as “on the job”. It is important to consider that education of modelers does not mainly happen in university courses and that the variety of model related jobs goes far beyond use for process design by consulting companies. To resolve the mismatch, the current connection between requirements for different jobs and the various types of education has to be assessed for different geographical regions and professional environments. This allows the evaluation and improvement of important educational paths, considering quality assurance and future developments. Moreover, conclusions from a workshop involving practitioners and academics from North America and Europe are presented. The participants stressed the importance of non-technical skills and recommended strengthening the role of realistic modeling experience in university training. However, this paper suggests that all providers of modeling education and support, not only universities, but also software suppliers, professional associations and companies performing modeling tasks are called to assess and strengthen their role in training and support of professional modelers.
Effects of acute hypoxia on the energy status and antioxidant defense system in the blood of carp - Cyprinvs carpio L.
Authors: Zikic Radoslav , Stajn Andras , Pavlovic Sladjan , Ognjanovic Branka , Maletic Snezana , Markovic Mirjana , Dragicevic-Djokovic Ljiljana , Radojicic Ratko , Saicic Zorica
Published: February 2009

The influence of acute hypoxia on glucose, pyruvate, lipid peroxide (LP) reduced glutathione (GSH) concentrations and lactate level in the whole blood of carp (Cyprinus carpio L) under aquarium conditions were studied. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), the concentrations of ATP and ADP and ATP/ADP ratio in the red blood cells (RBCs) were analyzed. Glutathione-S-transferase (GST) activity was determined in the plasma. Our experiments showed that short-term and long-term hypoxia causes significant changes of all examined haema-tological parameters. Increased concentration of LP and increased SOD CAT and GST activities, as well as a decreased GSH-Px activity showed that under hypoxic conditions oxidative stress and RBCs damage were produced.
A check-list of the harvestmen (Opilionida) from the Romanian caves
Authors: Ilie Victoria
Published: February 2009

A check-list of Opilionida species found until now in Romanian caves is presented. This survey is completed by a list of 182 caves from which the analyzed species were collected.
On the diversity of pseudoscorpions in Macedonia: Neobisium maksimtodorovici n. sp. Neobisiidae, Pseudoscorpiones
Authors: Curcic Bozidar , Dimitrijevic Rajko , Mihajlova Branislava
Published: February 2009

A new troglobitic species, Neobisium maksimtodorovici n. sp. (Neobisiidae Pseudoscorpiones), inhabiting the Momicek Cave, v. Belica, nr. Makedonski Brod, Macedonia, has been discovered and its diagnosis presented. In support of some earlier findings, this novelty points to the pronounced biodiversity of the area studied.
Contribution to the knowledge of longicorn beetles (Cerambycidae, Coleoptera) from Serbia, Montenegro, the Republic of Macedonia and Greece
Authors: Curcic Srecko , Brajkovic Miloje , Tomic Vladimir , Mihajlova Branislava
Published: February 2009

Altogether 63 species and 6 subspecies (classified into 43 genera) of longicorn beetles have been collected: 35 genera, 49 species and 5 subspecies have been recorded from Serbia, 11 genera and 13 species - from Montenegro, 5 genera, 5 species and 2 subspecies - from the Republic of Macedonia, and 3 genera and 3 species - from Greece. Of these taxa, there are no endemics to the area investigated. Additionally, one species of cerambycid beetles - Leiopus femoratus Fairmaire is registered for the first time for the territory of the former Yugoslavia. Further, four species of the longicorn beetles are new to the coleopteran fauna of Montenegro, two species - for Serbia and one species - for the Republic of Macedonia.
Callus induction and plant regeneration in the moss Aloina Aloides (Schultz) Kindb. (Pottiaceae, Bryopsida)
Authors: Bijelovic Aneta , Sabovljevic Marko
Published: February 2009

Callus induction of moss species Aloina aloides (Schultz) Kindb. was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) or with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin (KIN) or with 0.2 mg/L indole-3-butyric acid (IBA) and 2.0 mg/L 6-benzylaminopurine (BAP) or with 7.5 g/L of sucrose or with 15 g/L of sucrose or hormone - free and sugar free MS basal medium. The callus can be maintained for a long period of time without bud formation subcultured on the above media, at 16 h day/8 h night, 25 ? 2?C, 60-70% air humidity and irradiance of 50 ?mol m-2s-1. To obtain plant regeneration pieces, calli were transferred onto MS media supplemented with different concentrations of auxins and cytokinins (1.0 mg/L 2,4-D and 2 mg/L KIN; 0.2 mg/L IBA and 2 mg/L KIN; or 0.2 mg/L IAA and 2 mg/L BAP). In these media after subculturing, callus enlarges and turns to gametophytes with buds. Except for a smaller size, the plants obtained on the callus did not differ morphoanatomically from the shoots in the nature.
The iversity of the Chilopoda species from the karstic area of the Anina mountains (Banat, Romania)
Authors: Ilie Victoria , Negrea ŞT. , Mitic Bojan
Published: February 2009

This paper presents the most recent data about 23 centipede species collected from the edaphic and MSS environments from the Anina Mountains in the Romanian Banat.
Bioindication of air pollution in Nis by using epiphytic lichens
Authors: Stamenkovic S. , Cvijan Mirko
Published: February 2009

Bioindication of air pollution in the city of Nis, by using epiphytic lichens, was performed for the first time in 1992. In this paper the authors present the results of repeated investigations in 2002, by using new, modern method, and their comparison with the former investigations. In repeated investigations, on 59 investigated spots, the authors established the presence of 42 lichen taxa, 3 zones with different air pollution level ("lichens desert", "transitional zone" and "normal zone") and two subzones ("narrow transitional zone" and "wider transitional zone"), as well. As the most sensitive, some Melanelia-species (M. elegantula, M. exasperata, M. glabra), Evernia prunastr and Flavoparmelia caperata, were established while on the other hand, Phaeophyscia orbicularis, Physcia adscendens, Ph. stellaris and Lecanora argentata were the most tolerant.
The influence of dietary protein quality on midgut and brain proteins in Morimus funereus larvae
Authors: Ilijin Larisa , Jankovic-Tomanic Milena , Mrdakovic-Mitic Marija , Vlahovic Milena , Peric-Mataruga Vesna , Lazarevic Jelica , Nenadovic Vera
Published: February 2009

The response of starved Morimus funereus larvae refed with an artificial diet (Galford, 1967) was examined in the present paper. Larvae were offered diets varying in protein quality (soya protein, casein, and gelatin). Refeeding with the Galford diet (G I) and two modifications of it in which soya protein was supplemented with casein (G II) or gelatin (G III) led to an increase of body mass. Different protein quality in the nutritive substrate influenced both the quantity and quality of midgut and brain proteins.
Researches concerning the diversity and equitability of the Chilopoda populations from the deciduous forests
Authors: Gava R.
Published: February 2009

The author describes the Chilopoda associations from the durmast oak, beech and alder forests by the Motomura?s log-linear mathematical model. The Chilopoda associations present a diversity and equitability that is close enough to the log-linear mathematical model so they can be considered as nomocoenosis in the sense Daget, Lecordier and Leveque (1972).
Macroinvertebrates and fishes in the part of the Danube flowing through the Iron Gate national park and possibilities of their protection under in situ and ex situ conditions
Authors: Simic Vladica , Simic Snezana
Published: February 2009

Comparison of the results of later investigations of the Danube in the part flowing through in the Iron Gate (Djerdap) National Park with those of research conducted earlier (20 to 40 years ago) shows that changes have occurred in regard to the presence and especially the abundance of certain hydrobionts on this sector of the river, a finding that applies to all groups examined. The paper discusses the potential and results of conservation measures realized through both legal regulations and medium-term plans for the advancement of fishing in this region. In addition to in situ study during the period from 1999 to 2003, a large number of species (especially of macroinvertebrates and fish) were also investigated under artificial conditions (in the Kragujevac Aquarium) in order to gain a better understanding of their ecological characteristics, especially their sensitivity to various environmental stress factors. The presented results indicate that weight of specimens and success of culturing under ex situ conditions are correlated with their sensitivity under natural conditions.
On the diversity of Magdelainella Jeannel (Cholevidae, Coleoptera) in Serbia
Authors: Curcic Srecko , Brajkovic Miloje , Curcic Bozidar , Schönmann H.
Published: February 2009

Three new species of cholevid beetles, Magdelainella zivojindjordjevici n. sp. (southwestern Serbia), Magdelainella mucanjensis n. sp. (Mt. Mucanj Serbia), and Magdelainella nikolateslai n. sp. (Mt. Goc, Serbia) are described and diagnosed. All taxonomically important features (including female genitalia) are illustrated. Magdelainella noesskei (Apfelbeck) (from Bosnia and Herzegovina) is given full specific status and is clearly separated from M. serbica (M?ller) (from Serbia). All new species are clearly distinct from other existing congeners. These forms are of Tertiary origin and age and represent both relicts and endemics inhabiting southwestern and central parts of Serbia, respectively.
Kosaniniella javorensis n. gen., n. sp., from southwest Serbia, with notes on the evolutionary status of Knirschiella Guéorguiev, Cholevidae, Coleoptera
Authors: Curcic Srecko , Brajkovic Miloje , Curcic Bozidar , Senman H. , Makarov Slobodan , Mitic Bojan , Tomic Vladimir
Published: February 2009

A new genus and species of endogen cholevid beetles (Kosaniniella javorensis n. gen., n. sp) has been diagnosed and described from under stones in the valley of Suvodol, village of Debelja, near Nova Varos, Mt. Javor southwestern Serbia. This new genus clearly differs from all other closely related genera in the following correlative traits: Body size presence/absence of eye spots; length of distalmost antennomere; form of antennomere VIII; punctuation of elytra; presence/absence of sutural striae shape of elytra; form of aedeagus; shape of aedeagal sides; shape of parameral apex; form of paramerae; distribution of parameral setae; shape of anterior inner parameral seta; form of median part of copulatory piece length of basal bulbus; and form of spermatheca. Kosaniniella n. gen. belongs to a separate phyletic lineage which includes two other phenetically close genera, Magdelainella Jeannel and Knirschiella Gu?orguiev. The new genus is present in southwestern Serbia only, while Magdelainella inhabits Serbia and Bosnia and Herzegovina. Knirschiella is given full generic status in the present paper; its members are known from Albania. The Kosaniniella-Magdelainella-Knirschiella complex is probably of early Tertiary age, its species having originated during the Alpine Orogeny, which affected vast areas of the Balkan Peninsula, including the Dinarids otherwise their terra typica.
Taxonomic significance of micromorphology and dimensions of oospores in the genus Chara L., Charales, Chlorophyta
Authors: Mandal D.K. , Rej S.
Published: February 2009

Oospore wall ornamentation patterns as revealed by scanning electron microscopy (SEM) provide important taxonomic characters in the Charales. The present study illustrates inter- and intra-specific variations in the oospore wall ornamentation of five species of Chara, including eight intra-specific taxa. The type variety and form of C. globularis shows pusticular elevations with pores at the base of the elevation. The variety and form aspera of C. globularis shows a porous oospore wall, and the oospore is provided with a ribbon which bears nearly granular ornamentation. The type form of C. setosa has a granular oospore wall. The three forms of C. braunii show a similar type of ornamentation and support their placement under a single species. Chara corallina has a roughened oospore wall. Oospore dimensions studied at the population level are another feature included in the present study. The oospore dimension data (length, width fossa-breadth, number of striae) at the population level were analyzed statistically, and the results show species-specificity of these parameters. The significance of oospore wall ornamentation and dimensions are discussed simultaneously with morphological features.
Mitochondrial superoxide production and MnSOD activity following exposure to an agonist and antagonists of ionotropic receptors in rat brain
Authors: Radenovic Lidija , Selakovic Vesna
Published: February 2009

The involvement of NMDA and AMPA/kainate receptors in the induction of superoxide production in the rat brain was examined after intrahippocampal injection of kainate, a non-NMDA receptor agonist; kainate plus CNQX, a selective AMPA/kainate receptor antagonist; or kainate plus APV, a selective NMDA receptor antagonist. The measurements took place at different times in the ipsi- and contralateral hippocampus, forebrain cortex, striatum, and cerebellum homogenates. The used glutamate antagonists both ensured sufficient neuroprotection in the sense of lowering superoxide production and raising MnSOD levels, but in the mechanisms and time dynamics of their effects were different. Our findings suggest that NMDA and AMPA/kainate receptors are differentially involved in superoxide production.

 This article has been retracted. Link to the retraction 10.2298/ABS150318026E
Acute leukemia of childhood: A single institution's experience
Authors: Slavkovic Bojana , Guc-Scekic Marija , Bunjevacki Gordana , Djuricic S. , Krstic Aleksandra , Micic D. , Vujic Dragana , Kuzmanovic M. , Rasovic-Gvozdenovic Nada
Published: February 2009

The aim of this study was to investigate distribution of immunophenotypic and cytogenetic features of childhood acute leukemia (AL) in the cohort of 239 newly diagnosed patients registered at the leading pediatric oncohematology center in the country during a six-year period (1996-2002). With approximately 60-70% of all childhood AL cases in Serbia and Montenegro being diagnosed and treated in this institution the used data represent a valid research sample to draw conclusions for entire country. On the basis of five phenotypic markers, the distribution of immunological subtypes was as follows: 169 (70.7%) expressed B-cell marker CD19 (137 were CD10 positive and 32 CD10 negative), 37 (15.5%) belonged to T-lineage acute lymphoblastic leukemia (T-ALL) (cyCD3 positive), and 33 (13.8%) were acute myeloblastic leukemia (AML) (CD13 positive and/or CD33 positive in the absence of lymphoid-associated antigens). The ratio of males and females was 1.5:1. Most of the cases were between the ages of 2 and 4, and were predominantly B-lineage acute lymphoblastic leukemia (B-ALL) cases. Another peak of age distribution was observed at the age of 7. The frequency of T-ALL (18% of ALL) was similar to that reported for Mediterranean countries: France (19.4%), Greece (28.1%), Southern Italy (28.3%), and Bulgaria (28.0%). Cytogenetic analyses were performed in 193 patients: 164 ALL and 29 AML. Normal karyotype was found in 57% of ALL and in 55% of AML patients, while cytogenetic abnormalities including structural, numerical, and complex chromosomal rearrangements were found in 43% of ALL and in 45% of AML patients. Our results represent a contribution to epidemiological aspects of childhood leukemia studies.
Temperature and magnetic field effects on the activity of protocerebral neurosecretory neurons and corpora allata in Cerambyx cerdo L. larvae
Authors: Nenadovic Vera , Mrdakovic Marija , Lazarevic Jelica , Mircic D. , Todorovic Dajana , Prolic Zlatko
Published: February 2009

The effects of constant temperature and an extremely low-frequency magnetic field (ELFMF, 50 Hz and average induction of 20 mT) on the activity of medial protocerebral neurosecretory neurons (A1 and A2) and corpora allata were investigated in Cerambyx cerdo L. larvae after 30 days of exposure. Both constant temperature of 23?C and the ELFMF led to decrease in activity of A1 and A2 neurosecretory neurons and increase in activity of corpora allata compared to the control group (larvae from natural conditions). The changes are more pronounced in A2 than A1 neurons.
The counteracting effect of potassium cyanide in sodium azide-inhibited germination of Paulownia tomentosa Steud. seeds
Authors: Zivkovic Suzana , Grubisic Dragoljub , Giba Zlatko , Konjevic Radomir
Published: February 2009

The effect of some respiratory inhibitors on light-induced Paulownia tomentosa Steud. seed germination was studied. Millimolar solution of sodium azide was sufficient to completely prevent germination induced by a 5-min red light pulse. The inhibitory effect of azide was absent if seeds were rinsed before phytochrome activation by light. Sodium azide was effective only if present in the period of Pfr activity. The escape time from azide inhibition compared to the escape from far-red light action, was delayed for about 24 hours. When azide was applied after phytochrome activation, its effect depended on how long it was present in the incubation medium. The removal of azide allowed full restoration of germination by another red light pulse and the far-red escape time did not differ from the escape of untreated, i.e. water-imbibed seeds. Potassium cyanide alone did not produce any effect in light-stimulated germination of these seeds. However, it counteracted the inhibitory effect of azide in light-stimulated germination, if applied simultaneously at a concentration three times higher.
Revision of bank vole Clethrionomys glareolus (Schreber, 1780) (Mammalia, Rodentia) distribution in Serbia and Montenegro
Authors: Paunovic M. , Jovanovic Tatjana , Karapandza B. , Habijan-Mikes Vesna
Published: February 2009

The present article represents a complete review of all published data (with corrections) on bank vole Clethrionomys glareolus distribution in Serbia and Montenegro. On the other hand, data of 63 unpublished records stored in the period from 1956 to 1983 in the Mammal Study Collection of the Natural History Museum, Belgrade had not been processed until now. In the period from 1992 to 2004, 29 new findings were recorded, 12 of them outside the currently known area of distribution. New data reveal a wider distribution of bank vole than was known until now, completing and partly modifying previous knowledge about this rodent's bionomy and ecology in Serbia and Montenegro. The occurrence of bank vole in the Prokletije Mountains, Kosovo and Metohija represents its highest known altitude in Europe (2500 m). On the basis of these new data and observations, we can conclude that bank vole is continuously present in small and linear fragments of autochthonous woodlands on plains and hills, and that there are no large discontinuities in its distribution in Serbia and Montenegro, as was assumed earlier. In efforts to preserve overall biological diversity, the example of the bank vole underlines the need to intensify protection and management of woodlands especially remaining fragments of forests on plains and in hills.
7-nitroindazole, a selective neuronal nitric oxide synthase inhibitore in vivo, prevents kainate-induced intrahippocampal neurotoxicity
Authors: Radenovic Lidija , Selakovic Vesna , Bozic Biljana
Published: February 2009

We investigated the effects of 7-nitroindazole (7-NI), a selective neuronal nitric oxide synthase inhibitor in vivo, on nitrite concentration after kainic acid injection unilaterally into the CA3 region of the rat hippocampus. The accumulation of nitrite, the stable metabolite of NO, was measured by the Griess reaction at different times in hippocampus, forebrain cortex, striatum, and cerebellum homogenates. 7-nitroindazole can effectively inhibit NO synthesis in rat brain after kainate-induced neurotoxicity and suppressed nitrite accumulation. The present results suggest that neuronal NO synthase inhibitors may be useful in the treatment of neurological diseases in which excitotoxic mechanisms play a role.

 This article has been retracted. Link to the retraction 10.2298/ABS160412036E
Lignicolous fungi as potential natural sources of antioxidants
Authors: Karaman Maja , Mimica-Dukic Neda , Matavulj Milan
Published: February 2009

As a result of an interest in natural derived metabolites around the world higher fungi (Basidiomycotina) have taken on great importance in biochemical investigations. A large number of structurally divergent compounds - both cellular components and secondary metabolites - have been extracted and found to possess significant biological activity, such as an immunomodulative effect on the human body. Effects of fungal biomolecules as potential natural antioxidants have not been examined so far. Biochemical analysis have included in vitro testing of the influence of different extracts (water methanol, chloroform) of selected fungal sporocarps on Fe2+/ascorbate-induced lipid peroxidation (LP) in a lecithin liposome system by TBA assay, as well as various other procedures. Qualitative analysis by TLC revealed a distinction both between different extracts of the same fungal species and between similar extracts of different species. The results obtained on antioxidative activities (LP inhibition and "scavenging" activity) indicate that MeOH extracts manifested a degree of activity higher than that of CHCl3 extracts with respect to antioxidative activity, the extracts can be ranged in the following declining order: Ganoderma lucidum, Ganoderma applanatum Meripilus giganteus, and Flammulina velutipes. The obtained results suggest that the analyzed fungi are of potential interest as sources of strong natural antioxidants in the food and cosmetics industries, whereas synthetic ones have proved to be carcinogenic.

Detected autoplagiarism. Link to the Editorial Decision 10.2298/ABS1004251U
Influence of microclimatic conditions on nectar exudation in Glechoma hirsuta W. K
Authors: Macukanovic-Jocic Marina , Djurdjevic Lola
Published: February 2009

The nectar production of Glechoma hirsuta W. K. grown under different microclimatic habitat conditions was evaluated by determining the total daily nectar quantity per flower, diurnal dynamics of nectar secretion, the nectar secretion rate, and sugar concentration. Comparative analyses of nectar production in Glechoma hirsuta grown in a forest and on a test plot confirmed that this process varied as a function of microclimatic parameters (atmospheric humidity and air temperature), but did not reveal a close relationship between these parameters and sugar concentration in nectar. More intensive nectar secretion, with a decreasing tendency during the day, and higher total daily nectar volume per plant (1.603 ml/flower) were measured in the forest habitat. Diurnal variation in nectar production with two secretion peaks was found at both localities, and the secretion patterns were rather similar. Regarding the nectar secretion rate, G. hirsuta is a slow producer secreting less than 0.07 ml/h.
New longhorn beetles (Coleopterta: Cerambycidae) from Serbia and Montenegro
Authors: Pil Natasa , Stojanovic D.
Published: February 2009

Scientific analysis of longhorn beetles (Coleoptera: Cerambycidae) collected on the mountain Fruska Gora from 2000 to 2004 has shown the presence of six new species for the fauna of Serbia and Montenegro. In addition to these four species were new for the fauna of Serbia.
On the entomofauna of Mt. Durmitor (Northern Montenegro): Braconid wasps of the subfamily Opiinae (Braconidae, Hymenoptera)
Authors: Brajkovic M.M. , Nikolic Z. , Curcic Srecko
Published: February 2009

Braconids are primary parasites of other insects and their eggs, larvae, and adults, and species have been recently discovered that lay their eggs in plant seeds. Classified into about 25 genera, more than 1,400 species of Opiinae are known at the present time in the world fauna. They have been registered in all zoogeographic regions. The Opiinae are solitary endoparasites of the larvae of cyclorhaphous Diptera, most often those of species belonging to the families Agromyzidae, Tephritidae, Anthomyiidae Ephydridae. In investigations conducted on Mt. Durmitor since 1982, we have up to now established 10 species of braconids of the subfamily Opiinae (Opius peterseni Fi., O. caudatus Wesm., O. parvungula Th., O. levisWesm., O. pallipesWesm., O. quasiquisti Fi., O. exilis Hal., O. filicornis Th., O. lugens Hal., and O. meracus Fi), eight of which are new for the fauna of Serbia and Montenegro.
Implementation of GIS technologies in assessment and protection of natural values of Tara National Park
Authors: Radovic Dejan , Stevanovic Vladimir , Markovic D. , Jovanovic Slobodan , Dzukic G. , Radovic I.
Published: February 2009

Mount Tara is among the most important centers of Balkan and European ecosystem and species biodiversity. It is characterized by diversified and well-maintained communities of old deciduous and mixed coniferous forests (beech/fir/spruce). They represent a unique example of well preserved forests in SE Europe with numerous endemic and relict species of indigenous flora and fauna. The geographical information system (GIS) that we have created has proved an excellent tool for valorization and protection of all natural values and potentials of Tara National Park.
Does the coefficient of variation reflect developmental instability in plants?
Authors: Velickovic Miroslava , Stankovic Sinisa
Published: February 2009

Developmental stability or homeostasis refers to the ability of an individual to produce a consistent phenotype in a given environment (Graham et al. 1993). Reduced developmental stability can result from a wide variety of environmentally (or genetically) induced perturbations.
New evidence indicating the presence of the genus Branchipus schaeffer, 1766 (Crustacea, Branchiopoda) in the Eastern Balkans
Authors: Cvetkovic-Milicic Dragana , Petrov Brigita , Petrov Ivan
Published: February 2009

Widely distributed in Europe, the genus Branchipus is found only at several localities in the Balkans. One locality in Western Macedonia is inhabited by B. intermedius (the only known locality of this species known other than the type locality in Romania), the others by B. schaefferi.
Sympetrum depressiusculum (Sélys, 1841) new species in the Odonata fauna of Serbia
Authors: Santovac S. , Jovic M. , Andjus Ljiljana
Published: February 2009

The Hungarian investigator Janos Frivaldszky published results of faunal studies on territories that included some parts of the present Republic of Serbia (Frivaldszky, 1877). This paper reported, among others things, the finding of the species Sympetrum depressiusculum (S?lys, 1841) at the Uljma local?ity in South Banat. It represents the first data indicating the presence of this species in Serbia. For unknown reasons, this paper was not cited by the majority of workers that performed odonatological investigations in this region. Until the present time, there were no confirmations of the presence of this species either in Banat or in Serbia. The risk of oblivion of the finding published by Frivaldszky was suspended by recent findings of this species.
Photosensitive neurons in mollusks
Authors: Kartelija Gordana , Nedeljkovic M. , Radenovic Lidija
Published: February 2009

In addition to regular photoreceptors, some invertebrates possess simple extra ocular photoreceptors. For ex?ample, the central ganglia of mollusks contain photosensitive neurons. These neurons are located on the dorsal surface of the ganglia and based on their electrophysiological properties it has been postulated that they are internal photoreceptors. Besides the eye, transduction of light also occurs in these extra-ocular photoreceptors. In the present work, we analyze the reactivity of these nerve cells to light and describe the underlying mechanism mediating the light-induced response.

 This article has been retracted. Link to the retraction 10.2298/ABS150317025E
Kainate-induced oxidative stress and neurotoxicity in the rat brain
Authors: Radenovic Lidija , Selakovic Vesna
Published: February 2009

We investigated superoxide production and MnSOD activity after kainate injection into the CA3 region of the rat hippocampus. The measurements took place at different times in hippocampus, forebrain cortex, striatum, and cerebellum homogenates. Free radicals including superoxide are responsible for post-lesional cytotoxicity. Neuronal cells responded to oxidative stress in kainate-induced neurotoxicity and caused the protective mechanism to increase MnSOD levels. The increase of MnSOD in distinct brain regions functionally connected via afferents and efferent suggests that these regions are affected by the injury. It implies that MnSOD protects the cells in these regions from superoxide-induced damage and therefore may limit the retrograde and anterograde spread of neurotoxicity.

 This article has been retracted. Link to the retraction 10.2298/ABS150318027E
Antioxidant enzymes in the needles of different omorika lines
Authors: Bogdanovic Jelena , Ducic Tanja , Milosavic Nenad , Vujcic Z. , Sijacic Mirjana , Isajev Vasilije , Radotic Ksenija
Published: February 2009

Picea omorika (Panc) Purkyn? (Serbian spruce) is a Balkan endemic coniferous species. We studied soluble peroxidase, catalase, polyphenol oxidase, and superoxide-dismutase activity in the needles of five omorika lines grown in a generative seed orchard. The peroxidase and polyphenol oxidase isoenzyme patterns were also investigated. Activity of the studied enzymes varied among different lines. The highest activity of peroxidase, catalase and polyphenol oxidase was found in the A3 ("borealis") and B5 ("semidichotomous") lines. Four acidic and two basic peroxidase isoenzymes and one polyphenol oxidase isoenzyme were detected. There was no variation in either the peroxidase or the polyphenol oxidase isoenzyme pattern among the different lines.
Gagea spathacea (Hayne) Salisb. (Liliaceae): A new species for the flora of Serbia
Authors: Tomovic Gordana , Niketic M.
Published: February 2009

During field studies carried out in the territory of NW Serbia - the Pocerina region (Knic village) and surrounding of Valjevo (Divci village) in 2004 we collected some specimens of the genus Gagea and identified as G. spathacea (Hayne) Salisb. A review of the literature data revealed that this taxon has not been yet recorded for the flora of Serbia.
New longhorn beetles (Coleoptera: Cerambycidae) from Serbia
Authors: Pil Natasa , Stojanovic D.
Published: February 2009

The most recent data (Ilic, 2005) indicate the presence of 245 longhorn beetle species (Coleoptera: Cerambycidae) in Serbia. Not included in the mentioned publication, the following five species should be added to the list: Cortodera discolor Fairmaire, 1866; Stenopterus similatus Holzschuh 1979; Chlorophorus aegyptiacus (Fabricius, 1775); Agapanthia osmanlis (Reiche, 1858); Agapanthia maculicornis (Gyllenhal, 1817) (Pil and Stojanovic in press). A total number of 250 species are presently known for the Serbian longhorn beetle fauna.
Checklist of mosses of Croatia
Authors: Sabovljevic M.
Published: February 2009

Published data and available herbaria were used to compile a checklist of Croatian mosses. The flora of bryophytes of Croatia numbers 476 taxa. Among taxa known from Croatia, three are European endemic species and 25 taxa are of pan-European conservation interest.
Establishment and development of the Catherine’s moss Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) in in vitro conditions
Authors: Sabovljevic Aneta , Cvetic Tijana , Sabovljevic M.
Published: February 2009

The effect of sucrose and mineral salts on morphogenesis of the Catherine?s moss (Atrichum undulatum) in in vitro culture was tested. In vitro culture of this species was established from disinfected spores on Murashige and Skoog (MS) medium. Apical shoots of gametophytes were used to investigate the influence of sucrose and mineral salts on protonemal and gametophyte growth and multiplication. Paper also treats morpho-anatomical characteristics of plants grown in nature and plants derived from in vitro culture.
Vascular flora of the Uvac River Gorge in Serbia
Authors: Veljic M. , Marin P.D. , Krivosej Z. , Ljubic B.
Published: February 2009

During research on the vascular flora of the Uvac River Gorge, 730 species and infraspecific taxa from 87 families were found. The most numerous representatives were from the families Asteraceae (81 taxa), Fabaceae (57), Poaceae (55) and Lamiaceae (47). Hemicryptophytes were the dominant life form (56,6%); in the chorological sense, the most numerous were sub-Central-European (16,3 %) and Euro-Asian species (11,5 %). The following endemic species were recorded: Alyssum markgrafii, Valeriana simplicifolia, Cicerbita pancicii, Lamium bifidum balcanicum, Alyssum corymbosum, Fumana bonapartei, Euphorbia glabriflora, and Potentilla visianii.
Contribution to knowledge of the bryophyte flora of Backa (Vojvodina, Serbia)
Authors: Sabovljevic M. , Stevanovic V.
Published: February 2009

A contribution to knowledge of the poorly known bryophyte flora of Backa region in Serbia is given in the present paper. The total of bryophyte species recorded in the region is raised from seven to 46. Six nationally threatened and two species threatened on the European level are found in the investigated area.
Alteration of glucocorticoid receptor subcellular distribution by hyperthermic stress
Authors: Cvoro Aleksandra , Korac Aleksandra , Matic Gordana
Published: February 2009

The aim of the present study was to examine intracellular redistribution of the glucocorticoid receptor (GR) in rat liver cells during a 24-h time period after exposure of the animals to 41?C whole body hyperthermic stress. The level of the receptor protein in the cytoplasmic and nuclear compartments was measured by immunoblotting procedures applied to both crude cytosol and immunopurified GR, as well as by immunocytochemical analyses applied to both paraffin-embedded liver sections and unfixed nuclear smears. All the experimental approaches employed in the study provided similar results, demonstrating that the transient stress-related decline of the cytoplasmic GR observed during the first five hours after exposure of the animals to whole-body hyperthermic stress is accompanied by enhanced nuclear accumulation of the receptor. The study can contribute to a better understanding of the influence of stress on the glucocorticoid signal transduction pathway. .
Effect of acute adrenalectomy on rat liver glucocorticoid receptor
Authors: Isenovic Esma , Radojcic Marija , Zakula Zorica , Koricanac Goran , Ribarac-Stepic Nevena
Published: February 2009

In order to improve current clinical treatment of human hypocortisolism, it is necessary to understand molecular aspects of this pathophysiology. In this study liver tissues from male Wistar rats were used as an experimental model to study structural and functional properties of glucocorticoid receptor (GR) in the absence of glucocorticoid hormones (GC). Results show that acute adrenalectomy (ADX) significantly increases the number of GR binding sites and GR protein content. In addition, acute ADX stimulates increase in stability of the GR, decrease in stability of the glucocorticoid- receptor complex (G-R), and changes in accumulation of the G-R complex in nuclei and its cellular distribution. .