bluuebunny/crossref_metadata_2025 huggingface dataset:
Bioconversion of Tyrosine and Tryptophan Derived Biogenic Amines by Neuropathogenic Bacteria
Authors: Taj Aneela , Jamil Nusrat
Published: February 2018

The biochemical potential of pathogenic bacteria may cause alteration in the neurophysiological environment; consequently, neuroendocrine and immune responses of the host are modulated by endogenously produced metabolic products of neuropathogenic bacteria. The present study was designed to detect the derived biogenic amines in spent culture media of Bacillus cereus (Bc), Clostridium tetani (Ct), Listeria monocytogenes (Lm), and Neisseria meningitidis (Nm). Overnight grown culture in different culture media i.e., Nutrient broth (NB), Luria basal broth (LB), Brain Heart Infusion broth (BHI), and human serum supplemented RPMI 1640 medium (RPMI) were used to prepare filter-sterilized, cell-free cultural broths (SCFBs) and subjected to high performance liquid chromatography with electrochemical detection (HPLC-EC) along with the control SCFBs. Comparative analysis of biogenic amines in neuropathogenic bacterial SCFBs with their respective control (SCFB) revealed the complete degradation of dopamine (DA) into its metabolic products by Bc, Ct, and Nm, whereas Lm showed negligible degradation of DA. A relatively high concentration of 5-hydroxyindol acetic acid (5HIAA) by Bc in NB and LB indicated the tryptophan metabolism by the serotonin (5HT) pathway. Our study suggests that microbial endocrinology could help unravel new perspectives to the progression of infectious diseases.
Mechanochemical Functionalization of Carbon Black at Room Temperature
Authors: Leistenschneider Desirée , Zürbes Katharina , Schneidermann Christina , Grätz Sven , Oswald Steffen , Wegner Karl , Klemmed Benjamin , Giebeler Lars , Eychmüller Alexander , Borchardt Lars
Published: February 2018

Carbon nanomaterials such as carbon blacks are intrinsically hydrophobic with limited wettability in aqueous media, thus restricting their potential applications. To improve their hydrophilicity, common methods tend to utilize harmful chemicals and conditions, such as a mixture of KMnO4 and H2SO4 or a complex and expensive synthesis setup. In our work, we report a simple method to improve the wettability of these materials by a mechanochemical treatment completed within 1 h at room-temperature utilizing a NH3 solution. Besides increasing the specific surface area of the carbon black from 67 m2·g−1 up to 307 m2·g−1, our process also incorporates nitrogen- and oxygen-containing functional groups into the carbon. This reduces the contact angle from 80° to 30°, confirming an enhanced wettability. Our work presents an easy, fast, and straightforward pathway towards the functionalization of carbon nanomaterials and can be of use in various applications where aqueous wettability is advantageous.
Photocatalytic Reduction of CO2 from Simulated Flue Gas with Colored Anatase
Authors: Guan Yebin , Xia Ming , Marchetti Alessandro , Wang Xiaohong , Cao Weicheng , Guan Hanxi , Kong Xueqian
Published: February 2018

Photocatalytic reduction with sunlight is an economical and promising advanced approach for reducing the excessive emissions of CO2 from the combustion of fossil fuels. Aimed at practical applications, a type of inexpensive colored anatase material was used to evaluate CO2 photoreduction performance on a platform with a continuous flow of gas mixtures (10 vol % CO2, 90% N2), which resembles realistic flue gas conditions. The results showed an enhanced photocatalytic activity compared with standard P25 and significant improvement over pristine anatase. Based on a series of characterization techniques, we propose that the oxygen vacancies and surface hydroxyl groups on colored anatase can reduce the bandgap and assist the binding of CO2 molecules. Our results showed that photoreduction of CO2 is feasible under practical conditions, and the efficiency could be improved through modification of catalysts.
Users’ Perceptions Using Low-End and High-End Mobile-Rendered HMDs: A Comparative Study
Authors: Juan M.-Carmen , García-García Inmaculada , Mollá Ramón , López Richard
Published: February 2018

Currently, it is possible to combine Mobile-Rendered Head-Mounted Displays (MR HMDs) with smartphones to have Augmented Reality platforms. The differences between these types of platforms can affect the user’s experiences and satisfaction. This paper presents a study that analyses the user’s perception when using the same Augmented Reality app with two MR HMD (low-end and high-end). Our study evaluates the user’s experience taking into account several factors (control, sensory, distraction, ergonomics and realism). An Augmalpha-lowerented Reality app was developed to carry out the comparison for two MR HMDs. The application had exactly the same visual appearance and functionality for both devices. Forty adults participated in our study. From the results, there were no statistically significant differences for the users’ experience for the different factors when using the two MR HMDs, except for the ergonomic factors in favour of the high-end MR HMD. Even though the scores for the high-end MR HMD were higher in nearly all of the questions, both MR HMDs provided a very satisfying viewing experience with very high scores. The results were independent of gender and age. The participants rated the high-end MR HMD as the best one. Nevertheless, when they were asked which MR HMD they would buy, the participants chose the low-end MR HMD taking into account its price.
A Controller Design Based on Takagi-Sugeno Fuzzy Model Employing Trajectory of Partial Uncertainty
Authors: Han Hugang , Hamasaki Daisuke
Published: February 2018

When the Takagi–Sugeno (T-S) fuzzy model is used to design controllers for a concerned system, the discrepancy between the system and its T-S fuzzy model becomes crucial sometimes in terms of control performance, particularly in cases when the magnitude of the discrepancy is relatively large. While most existing works have focused on approaches to restrain the influence of the discrepancy, the idea used in this paper is to extract as much information from the discrepancy as possible at first and then use it in the controller design before restraining its influence. By doing so, the magnitude of the discrepancy is reduced accordingly, and thus, better control performance can be expected. Including the discrepancy and other uncertain elements like the inner parameters’ perturbation, a term called uncertainty is considered in this paper. Assuming that the uncertainty influences the system behavior through the state and control input, an observer able to catch the trajectory of the partial uncertainty related to the control input is proposed. Then, a controller employing the trajectory is suggested. All design parameters are obtained by solving certain linear matrix inequalities, which guarantees the system stability. Finally, simulations are provided to illustrate the effectiveness of the proposed approach.
Diaphragmatic Palsy
Authors: Kokatnur Laxmi , Rudrappa Mohan
Published: February 2018

The diaphragm is the primary muscle of respiration, and its weakness can lead to respiratory failure. Diaphragmatic palsy can be caused by various causes. Injury to the phrenic nerve during thoracic surgeries is the most common cause for diaphragmatic palsy. Depending on the cause, the symptoms of diaphragmatic palsies vary from completely asymptomatic to disabling dyspnea requiring mechanical ventilation. On pulmonary function tests, there will be a decrease in the maximum respiratory muscle power. Spirometry shows reduced lung functions and a significant drop of lung function in supine position is typical of diaphragmatic palsy. Diaphragmatic movements with respiration can be directly visualized by fluoroscopic examination. Currently, this test is being replaced by bedside thoracic ultrasound examination, looking at the diaphragmic excursion with deep breathing or sniffing. This test is found to be equally efficient, and without risks of ionizing radiation of fluoroscope. Treatment of diaphragmatic palsy depends on the cause. Surgical approach of repair of diaphragm or nonsurgical approach of noninvasive ventilation has been tried with good success. Overall prognosis of diaphragmatic palsy is good, except when it is related to neuromuscular degeneration conditions.
Adaptive Synchronization of Fractional-Order Complex-Valued Neural Networks with Discrete and Distributed Delays
Authors: Li Li , Wang Zhen , Lu Junwei , Li Yuxia
Published: February 2018

In this paper, the synchronization problem of fractional-order complex-valued neural networks with discrete and distributed delays is investigated. Based on the adaptive control and Lyapunov function theory, some sufficient conditions are derived to ensure the states of two fractional-order complex-valued neural networks with discrete and distributed delays achieve complete synchronization rapidly. Finally, numerical simulations are given to illustrate the effectiveness and feasibility of the theoretical results.
Sustainable Concrete Performance—CO2-Emission
Authors: Latawiec Rafal , Woyciechowski Piotr , Kowalski Karol
Published: February 2018

The balance of carbon dioxide emissions and other greenhouse gases in the life cycle of concrete is one of the important elements affecting the sustainable development of concrete technology. Modifications in the composition in the aim of minimization of so-called “carbon footprint” of concrete also affect the majority of its technical features, including primarily the mechanical properties and durability. The article presents a desirability function that would allow us to estimate the combined effect of the modification in terms of both CO2 emissions and some of the technical features of the concrete. As criterial features equivalent CO2 emission, compressive strength and susceptibility/resistance to concrete carbonation are selected. Selected features should be considered as an example for the presentation of the proposed methodology and represent the three pillars of concrete desirability in terms of sustainable development, i.e., the constructional usefulness, durability, and environmental performance.
Integrated Process for Extraction of Wax as a Value-Added Co-Product and Improved Ethanol Production by Converting Both Starch and Cellulosic Components in Sorghum Grains
Authors: Nghiem Nhuan , O’Connor James , Hums Megan
Published: February 2018

Grain sorghum is a potential feedstock for fuel ethanol production due to its high starch content, which is equivalent to that of corn, and has been successfully used in several commercial corn ethanol plants in the United States. Some sorghum grain varieties contain significant levels of surface wax, which may interact with enzymes and make them less efficient toward starch hydrolysis. On the other hand, wax can be recovered as a valuable co-product and as such may help improve the overall process economics. Sorghum grains also contain lignocellulosic materials in the hulls, which can be converted to additional ethanol. An integrated process was developed, consisting of the following steps: 1. Extraction of wax with boiling ethanol, which is the final product of the proposed process; 2. Pretreatment of the dewaxed grains with dilute sulfuric acid; 3. Mashing and fermenting of the pretreated grains to produce ethanol. During the fermentation, commercial cellulase was also added to release fermentable sugars from the hulls, which then were converted to additional ethanol. The advantages of the developed process were illustrated with the following results: (1) Wax extracted (determined by weight loss): ~0.3 wt % of total mass. (2) Final ethanol concentration at 25 wt % solid using raw grains: 86.1 g/L. (3) Final ethanol concentration at 25 wt % solid using dewaxed grains: 106.2 g/L (23.3% improvement). (4) Final ethanol concentration at 25 wt % solid using dewaxed and acid-treated grains (1 wt % H2SO4) plus cellulase (CTec2): 117.8 g/L (36.8% improvement).
The Financial Implications of a Well-Hidden and Ignored Chronic Lyme Disease Pandemic
Authors: Davidsson Marcus
Published: February 2018

1 million people are predicted to get infected with Lyme disease in the USA in 2018. Given the same incidence rate of Lyme disease in Europe as in the USA, then 2.4 million people will get infected with Lyme disease in Europe in 2018. In the USA by 2050, 55.7 million people (12% of the population) will have been infected with Lyme disease. In Europe by 2050, 134.9 million people (17% of the population) will have been infected with Lyme disease. Most of these infections will, unfortunately, become chronic. The estimated treatment cost for acute and chronic Lyme disease for 2018 for the USA is somewhere between 4.8 billion USD and 9.6 billion USD and for Europe somewhere between 10.1 billion EUR and 20.1 billion EUR. If governments do not finance IV treatment with antibiotics for chronic Lyme disease, then the estimated government cost for chronic Lyme disease for 2018 for the USA is 10.1 billion USD and in Europe 20.1 billion EUR. If governments in the USA and Europe want to minimize future costs and maximize future revenues, then they should pay for IV antibiotic treatment up to a year even if the estimated cure rate is as low as 25%. The cost for governments of having chronic Lyme patients sick in perpetuity is very large.
Effect of Irrigation on Growth, Yield, and Chemical Composition of Two Green Bean Cultivars
Authors: Saleh Said , Liu Guangmin , Liu Mingchi , Ji Yanhai , He Hongju , Gruda Nazim
Published: February 2018

A study was conducted in an environmentally controlled greenhouse to evaluate two green bean cultivars, ‘Bronco’ and ‘Paulista’, under three application volumes of irrigation water based on replacing 100, 80, and 60% of evapotranspiration (ET). The experiment was in a split-plot design with three replications, recording vegetative growth, yield, pod parameters, water use efficiency (WUE), and chemical content of pods. The results showed that there were no differences between 80% ET and 100% ET for most parameters. In addition, 80% of ET increased the pod yield and improved the pod parameters and chemical composition. Therefore, this irrigation treatment can increase green bean productivity and improve pod quality. Reducing water application from 100 to 60% of ET progressively increased WUE. The ‘Bronco’ cultivar had a higher plant height, pod yield, WUE, pod weight, pod diameter, and total fiber amount than ‘Paulista’, while the ‘Paulista’ cultivar was superior in total chlorophyll, number of pods per plant, pod length, P, Ca, Mg, Fe, Cu, protein, vitamin C, titratable acid, and soluble sugar.
New Insights into the Tumor Microenvironment Utilizing Protein Array Technology
Authors: Huang Wei , Luo Shuhong , Burgess Rob , Yi Yu-Hua , Huang Gordon , Huang Ruo-Pan
Published: February 2018

The tumor microenvironment (TME) is a considerably heterogeneous niche, which is created by tumor cells, the surrounding tumor stroma, blood vessels, infiltrating immune cells, and a variety of associated stromal cells. Intercellular communication within this niche is driven by soluble proteins synthesized by local tumor and stromal cells and include chemokines, growth factors, interferons, interleukins, and angiogenic factors. The interaction of tumor cells with their microenvironment is essential for tumorigenesis, tumor progression, growth, and metastasis, and resistance to drug therapy. Protein arrays enable the parallel detection of hundreds of proteins in a small amount of biological sample. Recent data have demonstrated that the application of protein arrays may yield valuable information regarding the structure and functional mechanisms of the TME. In this review, we will discuss protein array technologies and their applications in TME analysis to discern pathways involved in promoting the tumorigenic phenotype.
Handwritten Devanagari Character Recognition Using Layer-Wise Training of Deep Convolutional Neural Networks and Adaptive Gradient Methods
Authors: Jangid Mahesh , Srivastava Sumit
Published: February 2018

Handwritten character recognition is currently getting the attention of researchers because of possible applications in assisting technology for blind and visually impaired users, human–robot interaction, automatic data entry for business documents, etc. In this work, we propose a technique to recognize handwritten Devanagari characters using deep convolutional neural networks (DCNN) which are one of the recent techniques adopted from the deep learning community. We experimented the ISIDCHAR database provided by (Information Sharing Index) ISI, Kolkata and V2DMDCHAR database with six different architectures of DCNN to evaluate the performance and also investigate the use of six recently developed adaptive gradient methods. A layer-wise technique of DCNN has been employed that helped to achieve the highest recognition accuracy and also get a faster convergence rate. The results of layer-wise-trained DCNN are favorable in comparison with those achieved by a shallow technique of handcrafted features and standard DCNN.
Tribological Behavior of HNBR in Oil and Gas Field Applications
Authors: Balasooriya Winoj , Schrittesser Bernd , Wang Chao , Hausberger Andreas , Pinter Gerald , Schwarz Thomas
Published: February 2018

The common usages of elastomeric components in oil and gas field applications are in dynamic atmospheres; especially sealing appliances that are in relative motion when interacting with surfaces. Therefore, their performance and service life mainly depend on the wear and friction characteristics in use. The objective of this scientific work is to identify the effect of swelling-induced ageing on the tribological properties and surface damage mechanisms of hydrogenated nitrile butadiene rubber (HNBR) in contact with different liquids. Furthermore, the investigation of the co-relation between mechanical properties and surface properties in the tested conditions is indispensable. In the swollen state, deteriorated mechanical properties were observed; however, in de-swollen conditions, the mechanical properties were restored. As far as the surface characterization is concerned, when the HNBR was swollen by a standard IRM 903 solvent, its wear was greater compared with the un-swollen specimen (1.1 times) despite the lower coefficient of friction (COF) (reduced by ~25%) and surface temperature (reduced by ~2.4 °C). In the de-swollen condition, wear was even greater (6 times), but the COF and surface temperature were situated in between those recorded in the swollen and un-swollen conditions. With swelling, greater wear damage and lower COF were observed; higher surface ageing (softness), which eases crack growth, created bigger debris. Under the conditions used, in the de-swollen states, the bulk mechanical properties were almost recovered, in contrast to the surface properties, which were still significantly impaired.
Statics of Shallow Inclined Elastic Cables under General Vertical Loads: A Perturbation Approach
Authors: Luongo Angelo , Zulli Daniele
Published: February 2018

The static problem for elastic shallow cables suspended at points at different levels under general vertical loads is addressed. The cases of both suspended and taut cables are considered. The funicular equation and the compatibility condition, well known in literature, are here shortly re-derived, and the commonly accepted simplified hypotheses are recalled. Furthermore, with the aim of obtaining simple asymptotic expressions with a desired degree of accuracy, a perturbation method is designed, using the taut string solution as the generator system. The method is able to solve the static problem for any distributions of vertical loads and shows that the usual, simplified analysis is just the first step of the perturbation procedure proposed here.
Sliding Contact Wear Damage of EBM built Ti6Al4V: Influence of Process Induced Anisotropic Microstructure
Authors: Ryu Jae , Shrestha Sanjay , Manogharan Guha , Jung Jai
Published: February 2018

Process-induced directional microstructure is identified as one of the key factors of anisotropic mechanical properties. This directional property significantly affects surface contact fatigue and corrosion of electron beam melting (EBM) built biomedical implants. In the current study, material removal on EBM built titanium (Ti6Al4V) subjected to reciprocating motion of commercially pure titanium spherical slider is investigated to identify the influence of the process-induced layered structure and environments on wear damage. Specimens developed by two different build orientations are mechanically stimulated using different sliding directions with nominally elastic normal load in dry, passivating, and synovial environments. It was noticed that EBM orientation significantly changes wear behavior in ambient environment. Wear resistance of mill-annealed Ti6Al4V was improved in passivating environment. Implications to improve useful life of orthopedic implants are discussed.
Depression Mechanism of Strontium Ions in Bastnaesite Flotation with Salicylhydroxamic Acid as Collector
Authors: Cao Shiming , Cao Yijun , Liao Yinfei , Ma Zilong
Published: February 2018

Metal ions are widely present in flotation pulp. Metal ions change solution chemistry and mineral surface properties, consequently affecting mineral flotation. In this work, the effect of strontium ions on bastnaesite flotation with salicylhydroxamic acid (SHA) was investigated by microflotation tests, contact angle measurements, zeta-potential measurements, and X-ray photoelectron spectroscopy (XPS) analysis. Microflotation tests confirmed that the addition of strontium ions decreased bastnaesite floatability, compared with that in the absence of strontium ions. Contact angle measurements suggested that the pretreatment of strontium ions decreased SHA adsorption. Zeta potential measurements confirmed that the bastnaesite was depressed by the adsorption of positively charged strontium species, and the lower adsorption capacity of SHA onto the bastnaesite surfaces was obtained after modifying with strontium ions. XPS analysis demonstrated that strontium ions adsorbed onto the bastnaesite surfaces through the interaction between strontium ions and oxygen atoms of surface ≡ CeOH 0 groups. This occurrence hindered surface Ce sites which chelated with SHA and therefore, decreased bastnaesite floatability.
Nanoscale Study of Clausthalite-Bearing Symplectites in Cu-Au-(U) Ores: Implications for Ore Genesis
Authors: Owen Nicholas , Ciobanu Cristiana , Cook Nigel , Slattery Ashley , Basak Animesh
Published: February 2018

Symplectites comprising clausthalite (PbSe) and host Cu-(Fe)-sulphides (chalcocite, bornite, and chalcopyrite) are instructive for constraining the genesis of Cu-Au-(U) ores if adequately addressed at the nanoscale. The present study is carried out on samples representative of all three Cu-(Fe)-sulphides displaying clausthalite inclusions that vary in size, from a few µm down to the nm-scale (<5 nm), as well as in morphology and inclusion density. A Transmission Electron Microscopy (TEM) study was undertaken on foils prepared by Focussed Ion Beam and included atom-scale High-Angle Annular Dark-Field Scanning TEM (HAADF-STEM) imaging. Emphasis is placed on phase relationships and their changes in speciation during cooling, as well as on boundaries between inclusions and host sulphide. Three species from the chalcocite group (Cu2–xS) are identified as 6a digenite superstructure, monoclinic chalcocite, and djurleite. Bornite is represented by superstructures, of which 2a and 4a are discussed here, placing constraints for ore formation at T > 265 °C. A minimum temperature of 165 °C is considered for clausthalite-bearing symplectites from the relationships with antiphase boundaries in 6a digenite. The results show that alongside rods, blebs, and needle-like grains of clausthalite within the chalcocite that likely formed via exsolution, a second, overprinting set of replacement textures, extending down to the nanoscale, occurs and affects the primary symplectites. In addition, other reactions between pre-existing Se, present in solid solution within the Cu-(Fe)-sulphides, and Pb, transported within a fluid phase, account for the formation of composite, commonly pore-attached PbSe and Bi-bearing nanoparticles within the chalcopyrite. The inferred reorganisation of PbSe nanoparticles into larger tetragonal superlattices represents a link between the solid solution and the symplectite formation and represents the first such example in natural materials. Epitaxial growth between clausthalite and monazite is further evidence for the interaction between pre-existing Cu ores and fluids carrying REE, P, and most likely Pb. In U-bearing ores, such Pb can form via decay of uranium within the ore, implying hydrothermal activity after the initial ore deposition. The U-Pb ages obtained for such ores therefore need to be carefully assessed as to whether they represent primary ore deposition or, more likely, an overprinting event. A latest phase of fluid infiltration is the recognised formation of Cu-selenide bellidoite (Cu2Se), as well as Fe oxides.
Activity of Salvia dolomitica and Salvia somalensis Essential Oils against Bacteria, Molds and Yeasts
Authors: Ebani Valentina , Nardoni Simona , Bertelloni Fabrizio , Giovanelli Silvia , Ruffoni Barbara , D’Ascenzi Carlo , Pistelli Luisa , Mancianti Francesca
Published: February 2018

Essential oils (EOs) from Salvia dolomitica and Salvia somalensis, widely employed in the cosmetic and perfume industry, were analyzed for composition and tested against bacterial and fungal pathogens isolated from clinical and environmental specimens. The analyses were carried out against Staphylococcus aureus, Staphylococcus pseudointermedius, Pseudomonas aeruginosa, Escherichia coli, Streptococcus canis, Streptococcus pyogenes, Klebsiella pneumoniae, Proteus mirabilis, Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Aspergillus niger, Aspergillus flavus, Candida albicans, Candida krusei, Mucor sp. and Trichothecium roseum. Both EOs showed similar percentages of total monoterpenes and sesquiterpene hydrocarbons. The main constituents were 1,8-cineole and β-caryophyllene in S. dolomitica and bornyl acetate and camphor in S. somalensis. The selected EOs have no relevant antifungal or antibacterial activities if compared to conventional drugs.
Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak
Authors: Khalil Eilaf , Abd Manap Mohd , Mustafa Shuhaimi , Alhelli Amaal , Shokryazdan Parisa
Published: February 2018

Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains Lactobacillus rhmnosus (ATCC53103) and L. plantarum (ATCC8014). The seven isolated strains belonged to five different species—L. plantarum, L. fermentum, L. crispatus, L. reuteri, and L. pentosus—which were identified using API 50 CHL and 16S rRNA gene sequences (Polymerase chain reaction, PCR – based). The seven strains displayed different ability to produce EPS (100–850 mg/L). Isolates exhibited a high survivability to acid (pH 3.0), bile salts (0.3%), and gastrointestinal tract model (<70%). Results showed that the auto-aggregation and cell surface hydrophobicity ranged from 39.98% to 60.09% and 50.80% to 80.53%, respectively, whereas, the highest co-aggregation value (66.44%) was observed by L. fermentum (DUR8) with Pseudomonas aeruginosa. The isolates showed good inhibitory activity against tested pathogens, high antioxidant activity (32.29% to 73.36%), and good ability to reduce cholesterol (22.55% to 75.15%). Thus, the seven tested strains have value as probiotics.
Effects of Dried Blood Spot Storage on Lipidomic Analysis
Authors: Di Marino Cinzia , De Marco Anna , Pisanti Antonio , Romanucci Valeria
Published: February 2018

During the lipidomic analysis of red blood cell membranes, the distribution and percentage ratios of the fatty acids are measured. Since fatty acids are the key constituents of cell membranes, by evaluating their quantities it possible to understand the general health of the cells and to obtain health indicators of the whole organism. However, because the analysis is precise, it is necessary to ensure that the blood does not undergo significant variations between the point of collection and analysis. The composition of the blood may vary dramatically weeks after collection, hence, here an attempt is made to stabilize these complex matrixes using antioxidants deposited on the paper cards on which the blood itself is deposited.
Polysiloxane/Polystyrene Thermo-Responsive and Self-Healing Polymer Network via Lewis Acid-Lewis Base Pair Formation
Authors: Vidal Fernando , Lin Huina , Morales Cecilia , Jäkle Frieder
Published: February 2018

The use of thermo-reversible Lewis Pair (LP) interactions in the formation of transient polymer networks is still greatly underexplored. In this work, we describe the synthesis and characterization of polydimethylsiloxane/polystyrene (PDMS/PS) blends that form dynamic Lewis acid-Lewis base adducts resulting in reversible crosslinks. Linear PS containing 10 mol % of di-2-thienylboryl pendant groups randomly distributed was obtained in a two-step one-pot functionalization reaction from silyl-functionalized PS, while ditelechelic PDMS with pyridyl groups at the chain-termini was directly obtained via thiol-ene “click” chemistry from commercially available vinyl-terminated PDMS. The resulting soft gels, formed after mixing solutions containing the PDMS and PS polymers, behave at room temperature as elastomeric solid-like materials with very high viscosity (47,300 Pa·s). We applied rheological measurements to study the thermal and time dependence of the viscoelastic moduli, and also assessed the reprocessability and self-healing behavior of the dry gel.
Ent-Clerodane Diterpenes from the Bark of Croton oligandrus Pierre ex Hutch. and Assessment of Their Cytotoxicity against Human Cancer Cell Lines
Authors: Guetchueng Stephanie , Nahar Lutfun , Ritchie Kenneth , Ismail Fyaz , Evans Andrew , Sarker Satyajit
Published: February 2018

New clerodane diterpenes, 12-epi-megalocarpodolide D (2) and an epimeric mixture of crotonolins A (3) and B (4), were isolated from the bark of Croton oligandrus following a bioassay-guided isolation protocol. Known compounds, megalocarpodolide D (1), 12-epi-crotocorylifuran (5), cluytyl-ferulate (6), hexacosanoyl- ferulate (7), vanillin (8), acetyl-aleuritolic acid (9) and lupeol (10), were also isolated. The structures of the isolated compounds (1–10) were elucidated by spectroscopic means. The cytotoxicity of compounds 1–10 was assessed against A549, MCF7, PC3 and PNT2 cell lines using the MTT assay. Compounds 1 and 2 showed moderate levels of activity against both A549 and MCF7 cells with 1 being the most active with IC50 values of 63.8 ± 13.8 and 136.2 ± 22.7 µM against A549 and MCF7 cells, respectively. The epimeric mixture of 3 and 4 was moderately active against A549 and PC3 cells (IC50 = 128.6 ± 31.0 and 111.2 ± 2.9 µM, respectively).
Synthesis of Graphite Oxide with Different Surface Oxygen Contents Assisted Microwave Radiation
Authors: Ibarra-Hernández Adriana , Vega-Rios Alejandro , Osuna Velia
Published: February 2018

Graphite oxide is synthesized via oxidation reaction using oxidant compounds that have lattice defects by the incorporation of unlike functional groups. Herein, we report the synthesis of the graphite oxide with diverse surface oxygen content through three (B, C, D) different modified versions of the Hummers method assisted microwave radiation compared with the conventional graphite oxide sample obtained by Hummers method (A). These methods allow not only the production of graphite oxide but also reduced graphene oxide, without undergoing chemical, thermal, or mechanical reduction steps. The values obtained of C/O ratio were ~2, 3.4, and ~8.5 for methodologies C, B, and D, respectively, indicating the presence of graphite oxide and reduced graphene oxide, according to X-ray photoelectron spectroscopy. Raman spectroscopy of method D shows the fewest structural defects compared to the other methodologies. The results obtained suggest that the permanganate ion produces reducing species during graphite oxidation. The generation of these species is attributed to a reversible reaction between the permanganate ion with π electrons, ions, and radicals produced after treatment with microwave radiation.
Toll-Like Receptors: Regulators of the Immune Response in the Human Gut
Authors: Hug Hubert , Mohajeri M. , La Fata Giorgio
Published: February 2018

Toll-like receptors (TLRs) are powerful molecular regulators by which the immune system may “sense” the environment and protect the host from pathogens or endogenous threats. In mammalian cells, several TLRs were identified with a tissue and cell type-specific distribution. Understanding the functions of specific TLRs is crucial for the development and discovery of compounds useful to maintaining or re-establishing homeostasis in the gastrointestinal tract (GIT). Due to their relevance in regulating the inflammatory response in the GIT, we will focus here on TLR2, TLR4, and TLR5. In particular, we describe (a) the molecular pathways activated by the stimulation of these receptors with their known bacterial ligands; (b) the non-bacterial ligands known to interact directly with TLR2 and TLR4 and their soluble forms. The scope of this minireview is to highlight the importance of bacterial and non-bacterial compounds in affecting the gut immune functions via the activation of the TLRs.
Coordination Polymers Based on Phthalic Acid and Aminopyrazine Ligands: On the Importance of N–H···π Interactions
Authors: Hossain Anowar , Seth Saikat , Bauzá Antonio , Mukhopadhyay Subrata , Frontera Antonio
Published: February 2018

Two new Co(II) and Cu(II) coordination polymers, {Co(HL1)2(μ-L2)(H2O)2}n (1) and {[Cu(HL1)2(μ-L2)H2O]·H2O}n (2) (H2L1 = Phthalic acid and L2 = 2-aminopyrazine), have been synthesized by slow evaporation of solvent and characterized by IR spectroscopic, elemental, single-crystal X-ray diffraction and thermal analysis. X-ray results indicate that in both the polymers, phthalate acts as a monodentate ligand and the aminopyrazine ligand is responsible for the formation of the infinite one-dimensional chain structure. The solid-state structures are stabilized through hydrogen bonds and N‒H···π interactions by generating two-dimensional layered structures. Finally, the non-covalent interactions have been studied energetically and using Bader’s theory of atoms in molecules by means of Density Functional Theory (DFT) calculations. The influence of the metal coordination on the strength of the interaction has been studied using molecular electrostatic potential surface calculations.
Hydrogels-Assisted Cell Engraftment for Repairing the Stroke-Damaged Brain: Chimera or Reality
Authors: González-Nieto Daniel , Fernández-García Laura , Pérez-Rigueiro José , Guinea Gustavo , Panetsos Fivos
Published: February 2018

The use of advanced biomaterials as a structural and functional support for stem cells-based therapeutic implants has boosted the development of tissue engineering applications in multiple clinical fields. In relation to neurological disorders, we are still far from the clinical reality of restoring normal brain function in neurodegenerative diseases and cerebrovascular disorders. Hydrogel polymers show unique mechanical stiffness properties in the range of living soft tissues such as nervous tissue. Furthermore, the use of these polymers drastically enhances the engraftment of stem cells as well as their capacity to produce and deliver neuroprotective and neuroregenerative factors in the host tissue. Along this article, we review past and current trends in experimental and translational research to understand the opportunities, benefits, and types of tentative hydrogel-based applications for the treatment of cerebral disorders. Although the use of hydrogels for brain disorders has been restricted to the experimental area, the current level of knowledge anticipates an intense development of this field to reach clinics in forthcoming years.
The Virgin Mary in the Early Modern Italian Writings of Vittoria Colonna, Lucrezia Marinella, and Eleonora Montalvo
Authors: Haraguchi Jennifer
Published: February 2018

The Marian writings of the Roman poet Vittoria Colonna (1490/92–1547), the Venetian polemicist Lucrezia Marinella (1579–1653),1 and the Florentine educator Eleonora Montalvo (1602–1659) present an accessible model of the Virgin Mary in the early modern period that both lay and religious women could emulate in order to strengthen their individual spirituality. While the Catholic Church encouraged women to accept and imitate an ideal of the Virgin Mary’s character traits and behavior for the good of society, these three women writers constructed a more fruitful narrative of the Virgin’s life and experience that included elements and imagery that would empower women to enhance their personal practice of meditation.
A Note on Parameter Estimation in the Composite Weibull–Pareto Distribution
Authors: Calderín-Ojeda Enrique
Published: February 2018

Composite models have received much attention in the recent actuarial literature to describe heavy-tailed insurance loss data. One of the models that presents a good performance to describe this kind of data is the composite Weibull–Pareto (CWL) distribution. On this note, this distribution is revisited to carry out estimation of parameters via mle and mle2 optimization functions in R. The results are compared with those obtained in a previous paper by using the nlm function, in terms of analytical and graphical methods of model selection. In addition, the consistency of the parameter estimation is examined via a simulation study.
Multi-Temporal InSAR Structural Damage Assessment: The London Crossrail Case Study
Authors: Milillo Pietro , Giardina Giorgia , DeJong Matthew , Perissin Daniele , Milillo Giovanni
Published: February 2018

Spaceborne multi-temporal interferometric synthetic aperture radar (MT-InSAR) is a monitoring technique capable of extracting line of sight (LOS) cumulative surface displacement measurements with millimeter accuracy. Several improvements in the techniques and datasets quality led to more effective, near real time assessment and response, and a greater ability of constraining dynamically changing physical processes. Using examples of the COSMO-SkyMed (CSK) system, we present a methodology that bridges the gaps between MT-InSAR and the relative stiffness method for tunnel-induced subsidence damage assessment. The results allow quantification of the effect of the building on the settlement profile. As expected the greenfield deformation assessment tends to provide a conservative estimate in the majority of cases (~71% of the analyzed buildings), overestimating tensile strains up to 50%. With this work we show how these two techniques in the field of remote sensing and structural engineering can be synergistically used to complement and replace the traditional ground based analysis by providing an extended coverage and a temporally dense set of data.
Hydrogel-Based Fluorescent Dual pH and Oxygen Sensors Loaded in 96-Well Plates for High-Throughput Cell Metabolism Studies
Authors: Wu Shanshan , Wu Siying , Yi Zheyuan , Zeng Fei , Wu Weizhen , Qiao Yuan , Zhao Xingzhong , Cheng Xing , Tian Yanqing
Published: February 2018

In this study, we developed fluorescent dual pH and oxygen sensors loaded in multi-well plates for in-situ and high-throughput monitoring of oxygen respiration and extracellular acidification during microbial cell growth for understanding metabolism. Biocompatible PHEMA-co-PAM materials were used as the hydrogel matrix. A polymerizable oxygen probe (OS2) derived from PtTFPP and a polymerizable pH probe (S2) derived from fluorescein were chemically conjugated into the matrix to solve the problem of the probe leaching from the matrix. Gels were allowed to cure directly on the bottom of 96-well plates at room-temperature via redox polymerization. The influence of matrix’s composition on the sensing behaviors was investigated to optimize hydrogels with enough robustness for repeatable use with good sensitivity. Responses of the dual sensing hydrogels to dissolved oxygen (DO) and pH were studied. These dual oxygen-pH sensing plates were successfully used for microbial cell-based screening assays, which are based on the measurement of fluorescence intensity changes induced by cellular oxygen consumption and pH changes during microbial growth. This method may provide a real-time monitoring of cellular respiration, acidification, and a rapid kinetic assessment of multiple samples for cell viability as well as high-throughput drug screening. All of these assays can be carried out by a conventional plate reader.
Towards Efficient Wireless Body Area Network Using Two-Way Relay Cooperation
Authors: Waheed Maham , Ahmad Rizwan , Ahmed Waqas , Drieberg Micheal , Alam Muhammad
Published: February 2018

The fabrication of lightweight, ultra-thin, low power and intelligent body-borne sensors leads to novel advances in wireless body area networks (WBANs). Depending on the placement of the nodes, it is characterized as in/on body WBAN; thus, the channel is largely affected by body posture, clothing, muscle movement, body temperature and climatic conditions. The energy resources are limited and it is not feasible to replace the sensor’s battery frequently. In order to keep the sensor in working condition, the channel resources should be reserved. The lifetime of the sensor is very crucial and it highly depends on transmission among sensor nodes and energy consumption. The reliability and energy efficiency in WBAN applications play a vital role. In this paper, the analytical expressions for energy efficiency (EE) and packet error rate (PER) are formulated for two-way relay cooperative communication. The results depict better reliability and efficiency compared to direct and one-way relay communication. The effective performance range of direct vs. cooperative communication is separated by a threshold distance. Based on EE calculations, an optimal packet size is observed that provides maximum efficiency over a certain link length. A smart and energy efficient system is articulated that utilizes all three communication modes, namely direct, one-way relay and two-way relay, as the direct link performs better for a certain range, but the cooperative communication gives better results for increased distance in terms of EE. The efficacy of the proposed hybrid scheme is also demonstrated over a practical quasi-static channel. Furthermore, link length extension and diversity is achieved by joint network-channel (JNC) coding the cooperative link.
Polynomial Phase Estimation Based on Adaptive Short-Time Fourier Transform
Authors: Jing Fulong , Zhang Chunjie , Si Weijian , Wang Yu , Jiao Shuhong
Published: February 2018

Polynomial phase signals (PPSs) have numerous applications in many fields including radar, sonar, geophysics, and radio communication systems. Therefore, estimation of PPS coefficients is very important. In this paper, a novel approach for PPS parameters estimation based on adaptive short-time Fourier transform (ASTFT), called the PPS-ASTFT estimator, is proposed. Using the PPS-ASTFT estimator, both one-dimensional and multi-dimensional searches and error propagation problems, which widely exist in PPSs field, are avoided. In the proposed algorithm, the instantaneous frequency (IF) is estimated by S-transform (ST), which can preserve information on signal phase and provide a variable resolution similar to the wavelet transform (WT). The width of the ASTFT analysis window is equal to the local stationary length, which is measured by the instantaneous frequency gradient (IFG). The IFG is calculated by the principal component analysis (PCA), which is robust to the noise. Moreover, to improve estimation accuracy, a refinement strategy is presented to estimate signal parameters. Since the PPS-ASTFT avoids parameter search, the proposed algorithm can be computed in a reasonable amount of time. The estimation performance, computational cost, and implementation of the PPS-ASTFT are also analyzed. The conducted numerical simulations support our theoretical results and demonstrate an excellent statistical performance of the proposed algorithm.
The Disablement Score: An Intersubjective Severity Scale of the Social Exclusion of Disabled People
Authors: Sakakibara Kenjiro
Published: February 2018

If a disability is understood as a type of social exclusion, its severity can be gauged from the social aspect. Such measurement is necessary to explore the intersubjective structure of social exclusion associated with bodily functions and structures. This paper presents a sociological and statistical method to rate the severity of a disability as social exclusion. The method is modeled on the rating procedure of occupational prestige. According to this technique, people subjectively rate severity by answering a questionnaire. The ratings are converted into a score (the “disablement score”). The method is applied in a preliminary web survey. The reliability of the scale is examined. People evaluate various conditions very differently, with physical conditions with functional limitations rated as severe and disfigurements as mild. Although the result does not necessarily agree with the objective circumstances, it is meaningful in that it reflects people’s reactions and attitudes toward disabilities.
Differentiation in Higher Education: The Impact of Parental Education
Authors: Tsiplakides Iakovos
Published: February 2018

The widening of participation in higher education in recent decades has been heralded as a means toward the reduction of social class inequalities in higher education. Research findings indicate, though, that simply increasing the number of people attending higher education does not mean that social inequalities have been substantially reduced. The mass expansion of higher education has existed alongside a differentiated and stratified higher education. Students from more privileged socioeconomic backgrounds usually study in prestigious higher education institutions and departments which offer more ambitious occupational trajectories, while those from less privileged socioeconomic backgrounds usually attend lower status institutions and courses of study. Using official quantitative data, in this article we explore the correlation between familial cultural capital and distribution in higher education in Greece. The research findings show that the Greek higher education sector is differentiated, since students with parents who are higher education graduates are overrepresented in prestigious higher education departments and courses of study. Based on the research findings, we argue that initiatives to reduce social class inequalities in higher education need to tackle the issue of social class stratification in higher education and the unequal representation of people from different socioeconomic backgrounds in prestigious fields of study.