bluuebunny/crossref_metadata_2025 huggingface dataset:
Magnitude of anemia and associated factors among pregnant women attending antenatal care in governmental health facilities of Shashemene Town, Oromia region, Ethiopia
Authors: Nasir Mekiya , Ayele Habtamu Molla , Aman Rameto , Hussein Kelil
Published: September 2024

Introduction

Anemia during pregnancy is a common issue that significantly affects the health of both the mother and her child. Globally, anemia is a major public health concern, affecting both developing and developed countries, with approximately 1.3 billion people affected. Pregnant women are among the most vulnerable to anemia.

Objective

To assess the magnitude and risk factors of anemia among pregnant women attending antenatal care in Shashemene Town, Oromia, Ethiopia.

Methods

A facility-based cross-sectional study was conducted among 391 pregnant women in Shashemene Town in April 2022. Data were collected using interviewer-administered questionnaires, along with laboratory examinations of blood and stool samples. The data were entered into EpiData 3.1 and analyzed using the Statistical Package for Social Sciences (SPSS) version 22. Bivariate logistic regression was performed, and variables with a p-value of <0.25 were included in the multivariate logistic regression analysis to identify factors associated with anemia. Adjusted odds ratio (AOR) with 95% CIs were calculated, and a p-value of < 0.05 was considered statistically significant. Finally, the results are presented using narration, descriptive statistics, such as tables, graphs, and charts.

Results

The prevalence of anemia was found to be 30.9% (95% CI: 26.4, 35.4%). Factors significantly associated with a reduced risk of anemia included high dietary diversity (AOR = 0.217, 95% CI: 0.105–0.451), no history of excessive menstrual bleeding (AOR = 0.162, 95% CI 0.076–0.345), age 25–34 years (AOR = 0.391, 95% CI 0.173–0.883), and age ≥ 35 years (AOR = 0.068, 95% CI 0.011–0.444). Conversely, a mild upper arm circumference (MUAC) of <23 cm (AOR = 4.939, 95% CI 2.330–10.469), no use of contraceptives (AOR = 4.935, 95% CI 2.207–11.032), and no iron supplementation use (AOR = 3.588, 95% CI 1.794–7.175) were significantly associated with an increased risk of anemia.

Conclusion

According to the WHO classification, anemia in this study was found to be a moderate public health issue. High dietary diversity, no previous excessive menstrual bleeding, and age were significantly associated with a reduced risk of anemia, whereas a MUAC of <23 cm, no contraceptive use, and no iron supplementation were significantly associated with an increased risk of anemia. Therefore, promoting diverse diets among pregnant women, providing counseling on the benefits of family planning and iron-folic acid supplements, and improving women’s education and empowerment are essential.
Health-promoting behavior and its determinants towards non-communicable diseases among adult residents of the Gedeo zone, South Ethiopia: the application of the health belief model
Authors: Hareru Habtamu Endashaw , Mamo Tizalegn Tesfaye , Abebe Mesfin , Debela Berhanu Gidisa
Published: September 2024

Background

Non-communicable diseases are becoming a challenge for the health care system in Ethiopia, which has suffered a double burden from infectious and rapidly increasing non-communicable diseases. However, there is little information on health-promoting behavior in the study settings. Thus, the purpose of this study was to determine health-promoting behaviors and its associated factors among adult's residents of Gedeo zone.

Methods

A cross-sectional study was conducted among 705 adult residents of Gedeo zone, south Ethiopia, selected using a multi-stage sampling technique. Interviews administered through a structured questionnaire were used to collect the data. The data were entered using Kobo Collect and analyzed using Stata version 17. The baseline characteristics of the participants were summarized using descriptive statistics. The independent sample t-test and one-way ANOVA were used to compare two groups and more than two groups, respectively. Stepwise multiple linear regression analysis was used to identify the potential determinants of health-promoting behavior and its components. Statistically significant factors were declared at p-value of less than or equal to 0.05.

Results

The overall means score for health-promoting behavior was 73.88 ± 16.79. Physical activity and spiritual growth had the lowest and highest mean scores, respectively. The variables: gender, marital status, education, family history of NCDs, health insurance status, perceived health status, knowledge of NCD risk factors, risk perception of NCDs, expected outcome, cues to action, and self-efficacy showed a statistically significant difference in overall health-promoting behavior. The total health-promoting behavior score was associated with age, gender, perceived health status, marital status, family history of NCDs, health insurance, knowledge of NCD risk factors, perceived threat, expected outcome, self-efficacy, and cues to action.

Conclusion and recommendations

In the study, the mean score of health-promoting behaviors was low. Socio-demographic and economic variables, family history of NCD, perceived health status, knowledge of NCD risk factors, perceived threat, expected outcome, self-efficacy, and cues to action affect health-promoting behaviors. Therefore, the study suggests establishing health promotion programs to increase residents' awareness of health-promoting lifestyles, empower them to adopt healthy lifestyles, and improve health outcomes by increasing self-efficacy, providing education, and creating supportive environments.
Machine learning models can identify individuals based on a resident oral bacteriophage family
Authors: Mahmoudabadi Gita , Homyk Kelsey , Catching Adam B. , Mahmoudabadi Ana , Foley Helen Bermudez , Tadmor Arbel D. , Phillips Rob
Published: September 2024

Metagenomic studies have revolutionized the study of novel phages. However these studies trade depth of coverage for breadth. We show that the targeted sequencing of a small region of a phage terminase family can provide sufficient sequence diversity to serve as an individual-specific barcode or a “phageprint’’, defined as the relative abundance profile of the variants within a terminase family. By collecting ~700 oral samples from ~100 individuals living on multiple continents, we found a consistent trend wherein each individual harbors one or two dominant variants that coexist with numerous low-abundance variants. By tracking phageprints over the span of a month across ten individuals, we observed that phageprints were generally stable, and found instances of concordant temporal fluctuations of variants shared between partners. To quantify these patterns further, we built machine learning models that, with high precision and recall, distinguished individuals even when we eliminated the most abundant variants and further downsampled phageprints to 2% of the remaining variants. Except between partners, phageprints are dissimilar between individuals, and neither country-of-residence, genetics, diet nor cohabitation seem to play a role in the relatedness of phageprints across individuals. By sampling from six different oral sites, we were able to study the impact of millimeters to a few centimeters of separation on an individual’s phageprint and found that such limited spatial separation results in site-specific phageprints.
Haptics-mediated virtual embodiment: Impact of a wearable avatar-controlling system with kinesthetic gloves on embodiment in VR
Authors: Li Zhenxing , Bujić Mila , Buruk Oğuz ‘Oz’ , Bampouni Elpida , Järvelä Simo , Hamari Juho
Published: September 2024

Enhancing the experience of virtual reality (VR) through haptic feedback could benefit applications from leisure to rehabilitation and training. Devices which provide more realistic kinesthetic (force) feedback appear to hold more promise than their simpler vibrotactile counterparts. However, our understanding of kinesthetic feedback on virtual embodiment is still limited due to the novelty of appropriate kinesthetic devices. To contribute to the line of this research, we constructed a wearable system with state-of-the-art kinesthetic gloves for avatar full-body control, and conducted a between-subjects study involving an avatar self-touch task. We found that providing a kinesthetic sense of touch substantially strengthened the embodiment illusion in VR. We further explored the ability of these kinesthetic gloves to present virtual objects haptically. The gloves were found to provide useful haptic cues about the basic 3D structure and stiffness of objects for a discrimination task. This is one of the first studies to explore virtual embodiment by employing state-of-the-art kinesthetic gloves in full-body VR.
Vibration and Fault Analysis of a Rotor System of a Twin-Spool Turbo-Jet Engine in Ground Test
Authors: Huang Jingjing , Yang Yirong , Peng Bilian , Li Suobin
Published: September 2024

According to the characteristics of the rotor system in an aero-engine and the vibrational test requirements of the aero-engine ground test, suitable vibration measurement sensors and test positions were selected. The vibration signals at the casings for the compressor and turbine of a twin-spool turbo-jet engine were collected under the states of maximum power and afterburning respectively, and the power spectrum analysis was carried out to determine the positions and causes of vibration. Furthermore, methods and preventive measures for eliminating vibration have been proposed. The results indicated that the main rotor vibration excited by mass imbalance in the twin-spool turbo-jet engine was significant. Rotor spindle misalignment or rotor radial stiffness unevenness also induced the vibration. The aerodynamic pulse vibration formed by the rotor blades of the first stage of the low pressure compressor was large, and rub induced vibration fault may occur at the turbine rotor seals. Based on the power spectrum analysis technology, the rotor system faults information including the type, position, and the degree can be quickly identified, and useful attempts and explorations have been made to reduce the vibration faults of the twin-spool turbo-jet engine.
Comparative Analysis of Evapotranspiration Estimates: Applying Data from Meteorological Ground Station, ERA5-Land, and MODIS with ECOSTRESS Observations across Grasslands in Central-Western Poland
Authors: Dąbrowska-Zielińska Katarzyna , Panek-Chwastyk Ewa , Jurzyk Maciej , Wróblewski Konrad
Published: September 2024

The aim of this study was to analyze and compare evapotranspiration estimates obtained from different data sources over grassland regions in central-western Poland during the vegetation seasons in the years 2021 and 2022. The dataset provided includes evapotranspiration (ET) estimates derived from three sources: (1) evapotranspiration measurements from the ECOSTRESS satellite; (2) evapotranspiration estimates calculated using the energy balance method based on ERA5-Land meteorological data with land surface temperature (LST) from MODIS; and (3) evapotranspiration estimates with meteorological data derived from ground measurements replacing ERA5-Land data and using MODIS LST for the surface temperature. For the second and third sources, where the energy balance method (Penman–Monteith) was applied, the data used for the ET calculation were obtained from the nearest ground-based meteorological station to the test fields, with the most distant fields being up to 40 km away in a straight line. In addition, for comparison, the MOD16 global evapotranspiration product was added. In a study conducted in the central-western region of Poland, specifically in Wielkopolska (NUTS2–PL41), 18 grassland plots ranging in size from 0.36 to 21.34 ha were studied, providing valuable insights into the complex relationships between environmental parameters and evapotranspiration processes. The evapotranspiration derived from different sources was tested by applying correlation with soil moisture and the height of the grass obtained from ground measurements. It was found that the evapotranspiration data derived from ECOSTRESS had the best correlation with soil moisture (r = 0.46, p < 0.05) and the height of the grass (r = 0.45, p < 0.05), both of which were statistically significant. The values of the ground measurements (soil moisture and vegetation height were considered as verification for the evapotranspiration precision). In addition, the information about precipitation and air temperature during the time of measurements was considered as the verification for the evapotranspiration conditions. Comparisons between ECOSTRESS data and other sources suggest that ECOSTRESS measurements may offer the most precise estimates of evapotranspiration in the studied region.
Attraction of the Indian Meal Moth Plodia interpunctella (Lepidoptera: Pyralidae) to Commercially Available Vegetable Oils: Implications in Integrated Pest Management
Authors: Liu Junyan , Yu Zhuoer , He Xiong Zhao , Zhou Guoxin , Guo Mengbo , Deng Jianyu
Published: September 2024

The Indian meal moth, Plodia interpunctella, poses a significant threat to global agricultural products. Although pheromone-based technologies show promise for P. interpunctella management, limitations such as single-sex targeting necessitate exploring complementary strategies. Vegetable-based oils represent a potential alternative but their efficacy, sex-specificity, and interaction with sex pheromones remain unclear. To address these questions, we first examined the attraction of P. interpunctella female and male adults to 10 commercially available vegetable oils in semi-field conditions. Sesame, olive, and blended oils were the most attractive, capturing significantly more adults compared to other oils. We then evaluated the effectiveness of these three attractive oils and their combinations with P. interpunctella sex pheromones in a grain warehouse. Traps baited with these oils captured significantly more females and males compared to control traps without attractants; however, the addition of sex pheromones did not improve male capture and significantly reduced female capture, suggesting an inhibitory effect. Finally, we demonstrated that female mating status (mated vs. virgin) did not influence the attractiveness of traps baited with these effective oils. Our findings highlight the potential of standalone traps baited with blended, olive, or sesame oil as a practical, economic, and effective management strategy for P. interpunctella in storage facilities.
Research on the Mechanical Properties of Peanuts during the Harvesting Period
Authors: Shen Haiyang , Gu Man , Guo Kai , Ling Jie , Gu Fengwei , Pan Liang , Wu Feng , Hu Zhichao
Published: September 2024

To address the gap in research on the mechanical properties of peanuts in the harvesting period, the mechanical properties of peanut seedling vines, pods, and peduncles were studied during the peanut harvesting period. The moisture content of peanut pods, peduncles, and seedling vines was measured, yielding values of 36.03%, 66.76%, and 77.95%, respectively. The tensile characteristic parameters between the pods and peduncles, peduncles and peduncles, and the seedling vines and peduncles during the harvesting period were determined using an electronic universal testing machine at three different loading speeds: 10 mm/min, 20 mm/min, and 30 mm/min. The test results indicated that the peduncle–peduncle, peduncle–pod, and peduncle–seedling vine detachment forces were 19.91 N, 17.41 N, and 8.62 N. The mechanical properties of peanuts during the harvesting period differed from those of peanuts that were excavated and sun-dried. Peanut-digging and turning machines should be designed based on the detachment force required to separate the peduncles from the pods, which, at a loading speed of 20 mm/min, is 17 N. This examination of the mechanical properties of peanuts during the harvesting period could have significant practical implications and a lasting influence on enhancing the efficiency and quality of peanut harvesting, refining harvesting machinery design, and advancing agricultural modernization.
Optimal Effect of Substituting Organic Fertilizer for Inorganic Nitrogen on Yield and Quality of Winter Wheat under Drip Irrigation
Authors: Shi Changhai , Liao Anli , Du Chao , Li Lingyan , Wan Xuejie , Liu Yiguo
Published: September 2024

More than one-third of the global population relies on wheat as a staple food. To ultimately reduce inorganic nitrogen (N) usage through applying an organic fertilizer under drip irrigation and evaluate its effect on the yield, quality, and N utilization efficiency of winter wheat (variety Jimai 22) under various irrigation systems, an experiment was established and conducted in Yanghe Town, Jiaozhou City, from October 2020 to June 2022. The trial was designed with seven treatments, including a control (CK), to achieve a 25% total nitrogen reduction in all treatments except for CK. These treatments included drip irrigation with urea as CK, one-time application of urea through drip irrigation (FU1), one-time application of organic water-soluble fertilizer through furrow irrigation (FO1), one-time application of organic water-soluble fertilizer through drip irrigation (DO1), two-time application of organic water-soluble fertilizer through drip irrigation (DO2), one-time application of urea through drip irrigation (DU1), and two-time application of urea through drip irrigation (DU2). The results indicated that the application of a reduced N fertilizer plus an organic fertilizer significantly improved the dry matter accumulation (DMA) and the efficiency of N absorption and thus increased the grain yield. The DO2 treatment significantly exhibited a 15.5% and 16.9% increase in the DMA and the grain DMA in post-anthesis, respectively, compared to those of CK in the season of 2020–2021. Overall, the apparent nitrogen use efficiency with the drip irrigation topdressing treatments (DO1, DO2, DU1, DU2) increased significantly over two years in comparison with the urea fertilization through traditional furrow irrigation (CK), while the DO2 and DU2 treatments improved most significantly in the N use efficiency and N agronomic efficiency. Therefore, a reduced use of the inorganic N fertilizer with some organic fertilizers significantly increased the weight of thousand-grains and the yield of winter wheat, especially in the DO2 treatment, with an 11.7 t/ha and 10.9 t/ha increase, respectively, in both growing seasons of two years, while the DO2 treatment also improved the extensibility of wheat flour dough from grains harvested in both rainy (2020–2021) and less rainy (2021–2022) growing seasons. Therefore, we strongly recommend that two-time application of an organic water-soluble fertilizer through drip irrigation be the option to reduce the use of inorganic N fertilizers and increase the yield and quality of winter wheat under the conditions of this experiment.
Quantitative Trait Loci Mapping of First Pod Height in Faba Bean Based on Genotyping-by-Sequencing (GBS)
Authors: Lou Shubao , Teng Changcai , Hou Wanwei , Zhou Xianli , Zhang Hongyan , Liu Yujiao
Published: September 2024

Candidate genes related to first pod height (FPH) traits in faba bean plants are crucial for mechanised breeding. However, reports on quantitative trait locus (QTL) mapping related to the FPH of faba bean are few, thus limiting the high-quality development of the faba bean industry to a certain extent. The identification and screening of candidate genes related to FPH is extremely urgent for the advancement of mechanised breeding for faba bean. In this study, a high-density genetic linkage map was constructed using genotyping-by-sequencing (GBS) of an F3 population and QTLs (genes) related to FPH were identified. The genetic linkage map contained seven linkage groups with 3012 SNP markers with an overall length of 4089.13 centimorgan (cM) and an average marker density of 1.36 cM. Thirty-eight QTLs for the first pod node (FPN) and FPH were identified (19 each for FPN and FPH). The 19 QTLs associated with FPN were located on chromosomes 1L, 1S, 2, 3, 4, 5, and 6; the 19 QTLs associated with FPH were located on chromosomes 1L, 1S, 2, 3, 5, and 6. There was a co-localisation interval of qFPN6-1 and qFPH6-1 on chromosome 6. By annotating the QTL qFPH6-1 interval, 36 genes that may be related to FPH were identified, these genes are related to plant growth and development. The results provide a basis for the precise location of QTLs related to FPH and could accelerate the breeding of faba bean varieties adapted to mechanised harvesting.
The Application of Biochar Derived from Rice Husk Enhanced the Bioremediation of Petroleum-Contaminated Soil in Semi-Arid Areas
Authors: Liu Zhe , Zhang Yang , Li Xiumin , Sun Zenghui , Zhang Ruiqing , Li Xuxiang , Du Yichun
Published: September 2024

With the continuous expansion of petroleum exploitation, a large amount of petroleum hydrocarbons has leaked into the Earth’s soil, which seriously threatens ecological health. Hence, a series of experiments were conducted to evaluate the performance of natural attenuation (NA), rice husk (RH), and biochar derived from rice husk (RHBC) in enhancing the bioremediation of petroleum-contaminated soil. When the biodegradation time reached 100 d, the total petroleum hydrocarbons (TPHs) biodegradation amounts of NA, RH, and RHBC were 3688.2, 4557.9, and 5913.3 mg/kg, which was equal to the biodegradation efficiency of 32.8, 40.6, and 52.6%, respectively. Compared with NA and RH, the biodegradation efficiency of n-alkanes C11–C15 (C11 represents n-alkanes with 11 carbons, namely n-Hendecane) C16–C29, and C30–C40 in RHBC increased to 69.7~82.5%, 42.4~81.5%, and 30.2~48.1%, equivalent to 12.2~34.5%, 6.9~31.2%, and 13.5~37.1% more than NA and RH, respectively. Moreover, the half-life of biodegradation in RHBC was shorted to 83.3 d, equal to 49.7 and 67.9% of NA and RH, respectively. Furthermore, the application of RHBC could improve the dehydrogenase activity in soil and promote the growth and reproduction of petroleum-degrading bacteria (PDB), which enhances the biodegradation of petroleum hydrocarbons. This study exhibited that biochar formed from biomass pyrolysis is a potential biostimulator in enhancing the remediation of petroleum-contaminated soil by indigenous bacteria.
Integrating Heterosis for Root Architecture and Nitrogen Use Efficiency of Maize: A Comparison between Hybrids from Different Decades
Authors: Li Yuanyuan , Bai Lanfang , Wei Shuli , Wu Hao , Li Rongfa , Wang Yongqiang , Wang Zhigang
Published: September 2024

Exploring the biological potential of maize root architecture is one of the most important ways to improve nitrogen use efficiency (NUE). The NUE and its heterosis in maize hybrids have improved remarkably over decades. Yet, there is little research on maize hybrid heterosis for root architecture and its possible physiological relationship to heterosis for NUE. A field study lasting two years was carried out on four typical maize hybrids from old and new eras, including their parental inbred lines with two levels of nitrogen (0, 150 kg N ha−1). Compared to old-era maize hybrids, the brace root angle (BA) and crown root angle (CA) of new-era maize hybrids increased by 19.3% and 8.0% under 0 N, and by 18.8% and 7.9% under 150 N, which exhibited a steeper root architecture; the crown root number (CN) of new-era maize hybrids increased by 30.5% and 21.4% under 0 N and 150 N, respectively, which showed a denser root system; meanwhile, the depth of 95% cumulative root weight (D95) of new-era maize hybrids separately increased by 10.5% and 8.5% under 0 N and 150 N, which exhibited a deeper root distribution. This steeper-denser-deeper root architecture enhanced pre-anthesis N uptake and provided a premise of greater post-anthesis N remobilization. All maize hybrids displayed significant heterosis for root architecture compared to their parental inbred lines. The brace root branching (BB) and crown root branching (CB) of new-era maize hybrids and D95 have positive heterosis, while the BA, CA, and CB of old-era maize hybrids, brace root number (BN), and CN have negative heterosis. Regardless of whether root architecture heterosis was positive or negative, new-era maize hybrids showed an overall elevated trend compared to old-era maize hybrids. Structural equation modeling (SEM) showed that heterosis for nitrogen internal efficiency (NIE) was the primary reason for NUE heterosis in maize, influenced by heterosis for root architecture, which was steeper, denser, and deeper. Our results indicated that, compared with old-era maize hybrids, new-era maize hybrids had stronger heterosis for root architecture, which was beneficial to pre-silking nitrogen absorption and is an important physiological basis for the higher NIE heterosis and NUE heterosis in new-era maize hybrids.
Efficacy Evaluation of You Only Learn One Representation (YOLOR) Algorithm in Detecting, Tracking, and Counting Vehicular Traffic in Real-World Scenarios, the Case of Morelia México: An Artificial Intelligence Approach
Authors: Guzmán-Torres José A. , Domínguez-Mota Francisco J. , Tinoco-Guerrero Gerardo , García-Chiquito Maybelin C. , Tinoco-Ruíz José G.
Published: September 2024

This research explores the efficacy of the YOLOR (You Only Learn One Representation) algorithm integrated with the Deep Sort algorithm for real-time vehicle detection, classification, and counting in Morelia, Mexico. The study aims to enhance traffic monitoring and management by leveraging advanced deep learning techniques. The methodology involves deploying the YOLOR model at six key monitoring stations, with varying confidence levels and pre-trained weights, to evaluate its performance across diverse traffic conditions. The results demonstrate that the model is effective compared to other approaches in classifying multiple vehicle types. The combination of YOLOR and Deep Sort proves effective in tracking vehicles and distinguishing between different types, providing valuable data for optimizing traffic flow and infrastructure planning. This innovative approach offers a scalable and precise solution for intelligent traffic management, setting new methodologies for urban traffic monitoring systems.
The Anatomy, Histology, and Function of the Major Pelvic Ganglion
Authors: Landa-García Jessica Natalia , Palacios-Arellano María de la Paz , Morales Miguel Angel , Aranda-Abreu Gonzalo Emiliano , Rojas-Durán Fausto , Herrera-Covarrubias Deissy , Toledo-Cárdenas María Rebeca , Suárez-Medellín Jorge Manuel , Coria-Avila Genaro Alfonso , Manzo Jorge , Hernández-Aguilar Maria Elena
Published: September 2024

This review provides a comprehensive analysis of the pelvic plexus and its regulation across various mammalian species, including rats, cats, dogs, and pigs. The pelvic and hypogastric nerves play crucial roles in regulating pelvic functions such as micturition, defecation, and erection. The anatomical organization of these nerves varies, forming either well-defined ganglia or complex plexuses. Despite these variations, the neurons within these structures are consistently regulated by key neurotransmitters, norepinephrine and acetylcholine. These neurons also possess receptors for testosterone and prolactin, particularly in rats, indicating the significant role of these hormones in neuronal function and development. Moreover, neuropeptides such as vasoactive intestinal peptide (VIP), substance P, neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), and calcitonin gene-related peptide (CGRP) are co-released with neurotransmitters to modulate pelvic functions. This review highlights the complex interplay between neurotransmitters, neuropeptides, and hormones in regulating pelvic physiology and emphasizes the importance of hormonal regulation in maintaining the functionality and health of the pelvic plexus across different species.
A Comparison of Detour Behaviors in Some Marine and Freshwater Fish Species
Authors: Potrich Davide , Orsini Chiara , Stancher Gionata , Baratti Greta , Sovrano Valeria Anna
Published: September 2024

Evidence of detour ability to reach a salient goal in marine fishes (Chromis viridis, Chrysiptera parasema, Dascyllus aruanus) and freshwater fishes (Xenotoca eiseni, Danio rerio) has been observed using a “four-compartment box task” with an opaque barrier. The first experiment investigated this ability in marine fishes (Chromis viridis, Chrysiptera parasema, Dascyllus aruanus). Fish were placed in a four-compartment box, with social stimuli not accessible due to an opaque barrier. Two symmetrical apertures midline in the corridor allowed the fish to temporarily abandon the goal’s view and attempt to circumvent the barrier. Marine fish showed searching behavior in the two compartments near the social stimuli. In the second experiment, the detour abilities of a marine fish (Dascyllus aruanus) and two freshwater fishes (Xenotoca eiseni, Danio rerio) were compared using a modified version of the apparatus, with elongated compartments continuing further from the obstacle barrier and social stimuli. This enabled the evaluation of the dependence on effective distance to achieve the social goal. Both marine and freshwater fish exhibited detour skills. Additionally, Danio rerio’s differential spatial explorations inside compartments supported an active interest in searching for conspecifics, suggesting possible social object permanence retention. Overall, these results highlight the ecological salience of detour skills in fishes, irrespective of species-specific adaptations.
Nutritional Strategies to Alleviate Stress and Improve Welfare in Dairy Ruminants
Authors: Kotsampasi Basiliki , Karatzia Maria Anastasia , Tsiokos Dimitrios , Chadio Stella
Published: September 2024

Dairy ruminants provide a major part of the livestock and agriculture sectors. Due to the increase in world population and the subsequent increase in dairy product demands, the dairy sector has been intensified. Dairy farming intensification and the subsequent increase in animal nutritional demands and the increase in the average global temperature as well have subjected animals to various stress conditions that impact their health and welfare. Various management practices and nutritional strategies have been proposed and studied to alleviate these impacts, especially under heat stress, as well as during critical periods, like the transition period. Some of the nutritional interventions to cope with stress factors and ensure optimal health and production are the inclusion of functional fatty acids and amino acids and feed additives (minerals, prebiotics, probiotics, essential oils and herbs, phytobiotics, enzymes, etc.) that have been proven to regulate animals’ metabolism and improve their antioxidant status and immune function. Thus, these nutritional strategies could be the key to ensuring optimum growth, milk production, and reproduction efficiency. This review summarizes and highlights key nutritional approaches to support the remarkable metabolic adaptations ruminants are facing during the transition period and to reduce heat stress effects and evaluate their beneficial effects on animal physiology, performance, health, as well as welfare.
Co-Infection of Chickens with Staphylococcus lentus and Staphylococcus aureus from an Outbreak of Arthritis, Synovitis, and Osteomyelitis Argues for Detailed Characterisation of Isolates
Authors: Matos Miguel , Mitsch Peter , Liebhart Dieter , Hess Michael , Hess Claudia
Published: September 2024

Staphylococcus species are widespread in poultry environments and can cause various infections, often when the host’s defences are compromised. This manuscript reports on a co-infection of chickens with Staphylococcus lentus and Staphylococcus aureus associated with an outbreak of arthritis, synovitis, and osteomyelitis in an organic broiler breeder flock in Austria. Clinically, the affected flock showed weakness, lethargy, lameness, and increased mortality. Post-mortem examinations identified purulent arthritis and femoral head necrosis. Bacteriological analysis using MALDI-TOF MS identified both S. aureus and S. lentus in the affected joints. Antibiotic resistance testing revealed significant resistance, particularly in S. lentus. Histological analysis showed severe inflammation and bacterial colonies in the joints. While S. aureus is a common pathogen in poultry, S. lentus is less frequently reported. This study emphasises the need for detailed bacterial characterisation in outbreaks to better understand the role of less common pathogens like S. lentus. Further research is necessary to elucidate the impact of S. lentus on poultry health and its role in causing arthritis and synovitis, highlighting the importance of comprehensive investigation in such outbreaks.
Performance, Meat Quality and Meat Metabolomics Outcomes: Efficacy of 3-Nitrooxypropanol in Feedlot Beef Cattle Diets
Authors: Pedrini Cibeli Almeida , Machado Fábio Souza , Fernandes Alexandre Rodrigo Mendes , Cônsolo Nara Regina Brandão , Ocampos Fernanda Maria Marins , Colnago Luiz Alberto , Perdigão Alexandre , de Carvalho Victor Valério , Acedo Tiago Sabella , Tamassia Luis Fernando Monteiro , Kindermann Maik , Gandra Jefferson Rodrigues
Published: September 2024

30 Nellore animals with an average weight of 407.25 ± 2.04 kg, were distributed in a completely randomized design across the following treatments: 1—Control (without inclusion of 3-NOP); 2—BV75 (inclusion of 3-NOP at 75 mg/kg DM); 3—BV100 (inclusion of 3-NOP at 100 mg/kg DM). No significant effects were observed between treatments on ingestive behavior. However, the notable effect on the BWfinal and ADG of animals supplemented with 3-NOP compared to the control group was measurable. Cattle beef receiving 3-NOP exhibited reduced methane emissions (p < 0.0001) for all variables analyzed, resulting in an average decrease of 38.2% in methane emissions compared to the control, along with increased hydrogen emissions (g/day) (p < 0.0001). While supplementation with BV100 demonstrated lower methane emission, the performance was lower than BV75 in DMI, BWfinal, ADG, and ADG carcasses. Partial separation of metabolomics observed between groups indicated changes in meat metabolism when comparing the control group with the 3-NOP group, identifying metabolites with a variable importance projection (VIP) score > 1. In conclusion, supplementation with 3-NOP effectively reduced methane emissions and did not negatively influence animal performance.
Protective Effects of Tea Tree Oil on Inflammatory Injury of Porcine Intestinal Epithelial Cells Induced by Lipopolysaccharide In Vitro
Authors: Dong Li , Yuan Qingqing , Qiu Guangzhi , Zhang Yongsheng , Wang Hongrong , Yu Lihuai
Published: September 2024

Tea tree oil (TTO) improves the intestinal mucosal immunity of weaning piglets, but its underlying mechanism is not clear. We hypothesized that TTO may alleviate inflammatory injury by regulating the function of intestinal epithelial cells. Ileum epithelial cells (IPI-2I) were chosen and an inflammatory injury cell model was generated. The cell viability, cytokine secretion, and gene expression of TLR4 and NF-κB were measured to further evaluate the effects of TTO on the inflammatory injury in immune-stressed cells. The results showed that lipopolysaccharide (LPS; content: ≥30 μg/mL; time: 3 h, 6 h, or 9 h) decreased cell viability (p < 0.01), and 50 μg/mL LPS stimulated for 6 h resulted in an increased secretion of proinflammatory cytokines and a dramatically decreased secretion of anti-inflammatory cytokines (p < 0.05) in IPI-2I cells. Concentrations of 0–0.05% of TTO improved cell viability, while the 0.03% TTO treatment resulted in the highest cell viability and alleviated LPS-induced cell death (p < 0.01). In addition, 0.03% TTO alleviated the LPS-induced increase in the gene expression of IL-1β, TNFα, and IFNγ, as well as the decrease in the expression of IL-10 in IPI-2I cells (p < 0.05). LPS also upregulated the gene expression of TLR4 and NF-κB (p < 0.05); while TTO supplementation alleviated this effect (p < 0.05), 0.03% and 0.05% TTO supplementation had greater effects (p < 0.05). In conclusion, 50 μg/mL LPS stimulated for 6 h can be used to establish an immune-stressed cell model in IPI-2I cell lines, and 0.03% TTO treatment for 6 h alleviated inflammatory injury in the intestinal epithelial cells of pigs.
Farm-Level Risk Factors for Lameness in 659 German Dairy Herds Kept in Loose Housing Systems
Authors: Tillack Anna , Merle Roswitha , Müller Kerstin-Elisabeth , Hoedemaker Martina , Jensen Katharina Charlotte , Oehm Andreas W. , Klawitter Marcus , Stock Annegret
Published: September 2024

Six hundred fifty-nine farms in three regions of Germany (North: n = 240, East: n = 247, and South: n = 172) were included in the study, which aims at determining the association of management-related risk factors with farm-level lameness in German dairy herds. For each risk factor, a generalised linear regression model with negative binomial distribution and logit link was built. Results showed that cows housed in deep-bedded cubicles had a lower risk of being lame than cows housed in other cubicle types. A larger cubicle width was associated with a lower risk of being lame. Feeding a total mixed ration was associated with lower lameness prevalence (compared to feeding a partial mixed ration or single components). For first lactation cows, lameness assessment performed daily (compared to less than daily) and during other work tasks (compared to lameness assessment as a separate work task) were associated with lower risk for lameness. Finally, the present study provided evidence for crucial associations of management-related risk factors with lameness in German dairy cows, especially in the fields of cubicle design, feeding management, and lameness assessment.
Is Taurolidine Irrigation Effective in Preventing Surgical Site Infection during Fracture Surgery?
Authors: Dakhode Shubham Yashwant , Kim Woo Sub , Kim Hyun Jin , Lee Seung Yeol
Published: September 2024

Taurolidine, known for its broad-spectrum antimicrobial properties and low toxicity, has shown promise in reducing infections in various surgical settings. However, it has not been extensively evaluated in orthopedic surgery. This study assessed the efficacy of taurolidine irrigation in reducing surgical site infections in patients undergoing ankle fracture surgery. A retrospective review was conducted for patients >20 years old who underwent ankle fracture surgery between March 2016 and March 2023, with follow-ups exceeding 6 months. Patients were classified into the following two groups: those who underwent normal saline (NS) irrigation and those who underwent taurolidine irrigation. Minor infections were defined as requiring additional oral antibiotics postoperatively, while major infections were characterized by hospitalization or reoperation due to infection within 3 months. Of 844 patients, 688 were included. The taurolidine group (n = 328) had a significant reduction in minor infections (7.3% vs. 22.5%, odds ratio = 0.410, p = 0.028) compared to the NS group (n = 360). Major infections were fewer in the NS group (1.2% vs. 0%, p = 0.051), but the number of cases was too small for reliable analysis. Taurolidine irrigation significantly reduces the occurrence of minor infections in ankle fracture surgeries when compared to normal saline irrigation.
Incidence and Predictors of Healthcare-Associated Infections in Patients Admitted to a Temporary Intensive Care Unit during the COVID-19 Pandemic Waves: A Two-Year (2021–2023) Retrospective Cohort Study in Rome, Italy
Authors: Sciurti Antonio , Baccolini Valentina , Ceparano Mariateresa , Isonne Claudia , Migliara Giuseppe , Iera Jessica , Alessandri Francesco , Ceccarelli Giancarlo , Marzuillo Carolina , Tellan Guglielmo , De Giusti Maria , Pugliese Francesco , Villari Paolo ,   the Collaborating Group
Published: September 2024

To manage the number of critical COVID-19 patients, Umberto I Teaching Hospital in Rome established a temporary ICU on March 1, 2021. This study investigated the incidence and risk factors of healthcare-associated infections (HAIs) among these patients during various COVID-19 waves. Patients were grouped by admission date according to the dominant SARS-CoV-2 variant prevalent at the time (Alpha, Delta, Omicron BA.1, Omicron BA.2, Omicron BA.5, and Omicron XBB). First-HAI and mortality rates were calculated per 1000 patient-days. Predictors of first-HAI occurrence were investigated using a multivariable Fine–Gray regression model considering death as a competing event. Among 355 admitted patients, 27.3% experienced at least one HAI, and 49.6% died. Patient characteristics varied over time, with older and more complex cases in the later phases, while HAI and mortality rates were higher in the first year. Pathogens responsible for HAIs varied over time, with first Acinetobacter baumannii and then Klebsiella pneumoniae being progressively predominant. Multivariable analysis confirmed that, compared to Alpha, admission during the Omicron BA.1, BA.2, BA.5, and XBB periods was associated with lower hazards of HAI. Despite worsening COVID-19 patient conditions, late-phase HAI rates decreased, likely due to evolving pathogen characteristics, improved immunity, but also better clinical management, and adherence to infection prevention practices. Enhanced HAI prevention in emergency situations is crucial.
Exploring the Antioxidant and Anti-Inflammatory Potential of Saffron (Crocus sativus) Tepals Extract within the Circular Bioeconomy
Authors: Frusciante Luisa , Geminiani Michela , Shabab Behnaz , Olmastroni Tommaso , Scavello Giorgia , Rossi Martina , Mastroeni Pierfrancesco , Nyong’a Collins Nyaberi , Salvini Laura , Lamponi Stefania , Parisi Maria Laura , Sinicropi Adalgisa , Costa Lorenzo , Spiga Ottavia , Trezza Alfonso , Santucci Annalisa
Published: September 2024

Repurposing saffron (Crocus sativus) waste presents a sustainable strategy for generating high-value products within the bioeconomy framework. Typically, flower components are discarded after stigma harvest, resulting in significant waste—350 kg of tepals per kilogram of stigmas. This research employed a comprehensive approach, integrating bioactivity studies (in vitro and in silico) with Life Cycle Assessment (LCA) evaluations, to extract and assess bioactive compounds from C. sativus tepals sourced in Tuscany, Italy. Phytochemical characterization using UPLC-MS/MS revealed a high abundance and variety of flavonoids in the hydro-ethanolic extract (CST). The antioxidant capacity was validated through various assays, and the ability to mitigate H2O2-induced oxidative stress and enhance fermentation was demonstrated in Saccharomyces cerevisiae. This study reports that C. sativus tepals extract reduces oxidative stress and boosts ethanol fermentation in yeast, paving the way for applications in the food and biofuels sectors. Further validation in RAW 264.7 macrophages confirmed CST’s significant anti-inflammatory effects, indicating its potential for pharmaceutical, cosmeceutical, and nutraceutical applications. In silico studies identified potential targets involved in antioxidant and anti-inflammatory processes, shedding light on possible interaction mechanisms with Kaempferol 3-O-sophoroside (KOS-3), the predominant compound in the extract. The integration of LCA studies highlighted the environmental benefits of this approach. Overall, this research underscores the value of using waste-derived extracts through “green” methodologies, offering a model that may provide significant advantages for further evaluations compared to traditional methodologies and supporting the circular bioeconomy.
Multiple Mechanisms of Action of Sulfodyne®, a Natural Antioxidant, against Pathogenic Effects of SARS-CoV-2 Infection
Authors: Romeo Paul-Henri , Conquet Laurine , Messiaen Sébastien , Pascal Quentin , Moreno Stéphanie G. , Bravard Anne , Bernardino-Sgherri Jacqueline , Dereuddre-Bosquet Nathalie , Montagutelli Xavier , Le Grand Roger , Petit Vanessa , Ferri Federica
Published: September 2024

Few therapeutic options are available to treat COVID-19. The KEAP1/NRF2 pathway, the major redox-responsive pathway, has emerged as a potential therapeutic target for COVID-19 as it regulates redox homeostasis and inflammation that are altered during SARS-CoV-2 infection. Here, we characterized the effects of NRF2-agonist Sulfodyne®, a stabilized natural Sulforaphane, in cellular and animal models of SARS-CoV-2 infection. In pulmonary or colonic epithelial cell lines, Sulfodyne® elicited a more efficient inhibition of SARS-CoV-2 replication than NRF2-agonists DMF and CDDO. This antiviral activity was not dependent on NRF2 but was associated with the regulation of several metabolic pathways, including the inhibition of ER stress and mTOR signaling, which are activated during SARS-CoV-2 infection. Sulfodyne® also decreased SARS-CoV-2 mediated inflammatory responses by inhibiting the delayed induction of IFNB1 and type I IFN-stimulated genes in infected epithelial cell lines and by reducing the activation of human by-stander monocytes recruited after SARS-CoV-2 infection. In K18-hACE2 mice infected with SARS-CoV-2, Sulfodyne® treatment reduced both early lung viral load and disease severity by fine-tuning IFN-beta levels. Altogether, these results provide evidence for multiple mechanisms that underlie the antiviral and anti-inflammatory activities of Sulfodyne® and pinpoint Sulfodyne® as a potent therapeutic agent against pathogenic effects of SARS-CoV-2 infection.
TSG Extends the Longevity of Caenorhabditis elegans by Targeting the DAF-16/SKN-1/SIR-2.1-Mediated Mitochondrial Quality Control Process
Authors: Sun Menglu , Wei Congmin , Gao Yehui , Chen Xinyan , Zhong Kaixin , Li Yingzi , Yang Zhou , Gao Yihuai , Wang Hongbing
Published: September 2024

The improvement of mitochondrial function is described as a strategy for alleviating oxidative stress and intervening in the aging process. 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is one of the major bioactive components isolated from Polygonum multiflorum Thunb, and it exhibits multiple activities, including antioxidant and anti-inflammatory effects. In this study, we found that 200 μM TSG significantly extended the mean lifespan of Caenorhabditis elegans by 16.48% and improved health status by delaying age-associated physiological decline in worms. The longevity prolongation effect of TSG depended on the regulation of the mitochondrial quality control process mediated by DAF-16/FOXO, SKN-1/Nrf2 and SIR-2.1/SIRT1 to improve mitochondrial function. Moreover, TSG treatment obviously alleviated the proteotoxicity of β-amyloid and tau proteins in worms. Our findings indicated that TSG is a promising natural product for preventing aging and treating aging-associated neurodegenerative diseases by regulating the mitochondrial quality control process to improve mitochondrial function.