bluuebunny/crossref_metadata_2025 huggingface dataset:
Omeprazole Inhibits Glioblastoma Cell Invasion and Tumor Growth
Authors: Jin Un-Ho , Michelhaugh Sharon K. , Polin Lisa A. , Shrestha Rupesh , Mittal Sandeep , Safe Stephen
Published: July 2020

Background: The aryl hydrocarbon receptor (AhR) is expressed in gliomas and the highest staining is observed in glioblastomas. A recent study showed that the AhR exhibited tumor suppressor-like activity in established and patient-derived glioblastoma cells and genomic analysis showed that this was due, in part, to suppression of CXCL12, CXCR4 and MMP9. Methods: Selective AhR modulators (SAhRMs) including AhR-active pharmaceuticals were screened for their inhibition of invasion using a spheroid invasion assay in patient-derived AhR-expressing 15-037 glioblastoma cells and in AhR-silenced 15-037 cells. Invasion, migration and cell proliferation were determined using spheroid invasion, Boyden chambers and scratch assay, and XTT metabolic assays for cell growth. Changes in gene and gene product expression were determined by real-time PCR and Western blot assays, respectively. In vivo antitumorigenic activity of omeprazole was determined in SCID mice bearing subcutaneous patient-derived 15-037 cells. Results: Results of a screening assay using patient-derived 15-037 cells (wild-type and AhR knockout) identified the AhR-active proton pump inhibitor omeprazole as an inhibitor of glioblastoma cell invasion and migration only AhR-expressing cells but not in cells where the AhR was downregulated. Omeprazole also enhanced AhR-dependent repression of the pro-invasion CXCL12, CXCR4 and MMP9 genes, and interactions and effectiveness of omeprazole plus temozolomide were response-dependent. Omeprazole (100 mg/kg/injection) inhibited and delayed tumors in SCID mice bearing patient-derived 15-037 cells injected subcutaneously. Conclusion: Our results demonstrate that omeprazole enhances AhR-dependent inhibition of glioblastoma invasion and highlights a potential new avenue for development of a novel therapeutic mechanism-based approach for treating glioblastoma.
The HECT E3 Ligase E6AP/UBE3A as a Therapeutic Target in Cancer and Neurological Disorders
Authors: Owais Asia , Mishra Rama K. , Kiyokawa Hiroaki
Published: July 2020

The HECT (Homologous to the E6-AP Carboxyl Terminus)-family protein E6AP (E6-associated protein), encoded by the UBE3A gene, is a multifaceted ubiquitin ligase that controls diverse signaling pathways involved in cancer and neurological disorders. The oncogenic role of E6AP in papillomavirus-induced cancers is well known, with its action to trigger p53 degradation in complex with the E6 viral oncoprotein. However, the roles of E6AP in non-viral cancers remain poorly defined. It is well established that loss-of-function alterations of the UBE3A gene cause Angelman syndrome, a severe neurodevelopmental disorder with autosomal dominant inheritance modified by genomic imprinting on chromosome 15q. Moreover, excess dosage of the UBE3A gene markedly increases the penetrance of autism spectrum disorders, suggesting that the expression level of UBE3A must be regulated tightly within a physiologically tolerated range during brain development. In this review, current the knowledge about the substrates of E6AP-mediated ubiquitination and their functions in cancer and neurological disorders is discussed, alongside with the ongoing efforts to pharmacologically modulate this ubiquitin ligase as a promising therapeutic target.
MicroRNA-21-Enriched Exosomes as Epigenetic Regulators in Melanomagenesis and Melanoma Progression: The Impact of Western Lifestyle Factors
Authors: Melnik Bodo C. , John Swen Malte , Carrera-Bastos Pedro , Schmitz Gerd
Published: July 2020

DNA mutation-induced activation of RAS-BRAF-MEK-ERK signaling associated with intermittent or chronic ultraviolet (UV) irradiation cannot exclusively explain the excessive increase of malignant melanoma (MM) incidence since the 1950s. Malignant conversion of a melanocyte to an MM cell and metastatic MM is associated with a steady increase in microRNA-21 (miR-21). At the epigenetic level, miR-21 inhibits key tumor suppressors of the RAS-BRAF signaling pathway enhancing proliferation and MM progression. Increased MM cell levels of miR-21 either result from endogenous upregulation of melanocytic miR-21 expression or by uptake of miR-21-enriched exogenous exosomes. Based on epidemiological data and translational evidence, this review provides deeper insights into environmentally and metabolically induced exosomal miR-21 trafficking beyond UV-irradiation in melanomagenesis and MM progression. Sources of miR-21-enriched exosomes include UV-irradiated keratinocytes, adipocyte-derived exosomes in obesity, airway epithelium-derived exosomes generated by smoking and pollution, diet-related exosomes and inflammation-induced exosomes, which may synergistically increase the exosomal miR-21 burden of the melanocyte, the transformed MM cell and its tumor environment. Several therapeutic agents that suppress MM cell growth and proliferation attenuate miR-21 expression. These include miR-21 antagonists, metformin, kinase inhibitors, beta-blockers, vitamin D, and plant-derived bioactive compounds, which may represent new options for the prevention and treatment of MM.
Multiple Myeloma Associated Bone Disease
Authors: Rasch Stine , Lund Thomas , Asmussen Jon Thor , Lerberg Nielsen Anne , Faebo Larsen Rikke , Østerheden Andersen Mikkel , Abildgaard Niels
Published: July 2020

The lytic bone disease is a hallmark of multiple myeloma, being present in about 80% of patients with newly diagnosed MM, and in more during the disease course. The myeloma associated bone disease (MBD) severely affects the morbidity and quality of life of the patients. MBD defines treatment demanding MM. In recent years, knowledge of the underlying pathophysiology has increased, and novel imaging technologies, medical and non-pharmaceutical treatments have improved. In this review, we highlight the major achievements in understanding, diagnosing and treating MBD. For diagnosing MBD, low-dose whole-body CT is now recommended over conventional skeletal survey, but also more advanced functional imaging modalities, such as diffusion-weighted MRI and PET/CT are increasingly important in the assessment and monitoring of MBD. Bisphosphonates have, for many years, played a key role in management of MBD, but denosumab is now an alternative to bisphosphonates, especially in patients with renal impairment. Radiotherapy is used for uncontrolled pain, for impeding fractures and in treatment of impeding or symptomatic spinal cord compression. Cement augmentation has been shown to reduce pain from vertebral compression fractures. Cautious exercise programs are safe and feasible and may have the potential to improve the status of patients with MM.
Development of a Novel Method for the Fabrication of Nanostructured Zr(x)Ni(y) Catalyst to Enhance the Desorption Properties of MgH2
Authors: Shokano Gracia , Dehouche Zahir , Galey Basile , Postole Georgeta
Published: July 2020

The present work involves the development of a novel method for the fabrication of zirconium nickel (Zr(x)Ni(y)) alloy used as a nanocatalyst to improve the hydrogen storage properties of the Mg/MgH2 system. The catalyst was fabricated through the high-pressure reactor and activated under hydrogen prior to being mechanically milled with the MgH2 for 5 h under argon. The microstructure characterisation of the samples was determined via SEM-EDX (scanning electron microscope analysis–energy dispersive X-ray spectroscopy), XRD (X-ray diffraction) and FE-HRTEM (field emission high resolution transmission electron microscopy), and the desorption characteristic of the nanocomposite (10 wt.% Zr(x)Ni(y)–MgH2) was determined via TPD (temperature-programmed desorption). The nanostructured MgH2 powder milled with 10 wt.% of the activated Zr(x)Ni(y) based nanocatalyst resulted in a faster hydrogen release—5.9 H2-wt.% at onset temperature 210 °C/peak temperature 232 °C. The observed significant improvement in the hydrogen desorption properties was likely to be the result of the impact of the highly dispersed catalyst on the surface of the Mg/MgH2 system, the reduction in particle size during the ball milling process and/or the formation of Mg0.996Zr0.004 phase during the milling process.
Transition Metal-Catalyzed α-Position Carbon–Carbon Bond Formations of Carbonyl Derivatives
Authors: Lee Ha-Eun , Kim Dopil , You Ahrom , Park Myung Hwan , Kim Min , Kim Cheoljae
Published: August 2020

α-Functionalization of carbonyl compounds in organic synthesis has traditionally been accomplished via classical enolate chemistry. As α-functionalized carbonyl moieties are ubiquitous in biologically and pharmaceutically valuable molecules, catalytic α-alkylations have been extensively studied, yielding a plethora of practical and efficient methodologies. Moreover, stereoselective carbon–carbon bond formation at the α-position of achiral carbonyl compounds has been achieved by using various transition metal–chiral ligand complexes. This review describes recent advances—in the last 20 years and especially focusing on the last 10 years—in transition metal-catalyzed α-alkylations of carbonyl compounds, such as aldehydes, ketones, imines, esters, and amides and in efficient carbon–carbon bond formations. Active catalytic species and ligand design are discussed, and mechanistic insights are presented. In addition, recently developed photo-redox catalytic systems for α-alkylations are described as a versatile synthetic tool for the synthesis of chiral carbonyl-bearing molecules.
Improved Electrocatalytic Activity and Durability of Pt Nanoparticles Supported on Boron-Doped Carbon Black
Authors: Yao Rui , Gu Jun , He Haitong , Yu Tao
Published: August 2020

A facile strategy is proposed to synthesize boron-doped ECP600 carbon black (B-ECP600), and the catalyst of Pt supported on boron-doped ECP600 (Pt/B-ECP600) shows smaller particle sizes and a higher electrochemical surface area (95.62 m2·gPt−1) and oxygen reduction reaction activity (0.286 A·mgPt−1 for mass activity; 0.299 mA·cm−2 for area specific activity) compared to the catalyst of Pt supported on ECP600 (Pt/ECP600). The results show that the boron doping of the carbon supports plays an important role in controlling the size and dispersion of Pt nanoparticles and the O2 adsorption/dissociation of the oxygen reduction reaction. A further accelerated durability test proves that boron doping can greatly enhance the stability of carbon support and thus improves the electrochemical performance of the catalyst during the long-time running. All these results suggest boron-doped carbon has great potential for application in fuel cells.
N8− Polynitrogen Stabilized on Nitrogen-Doped Carbon Nanotubes as an Efficient Electrocatalyst for Oxygen Reduction Reaction
Authors: Yao Zhenhua , Fan Ruiyang , Ji Wangyang , Yan Tingxuan , Hu Maocong
Published: August 2020

In this work, non-traditional metal-free polynitrogen chain N8− deposited on a nitrogen-doped carbon nanotubes (PN-NCNT) catalyst was successfully synthesized by a facile cyclic voltammetry (CV) approach, which was further tested in an oxygen reduction reaction (ORR). The formation of PN on NCNT was confirmed by attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR) and Raman spectroscopy. Partial positive charge of carbon within NCNT facilitated electron transfer and accordingly induced the formation of more PN species compared to CNT substrate as determined by temperature-programmed decomposition (TPD). Rotating disk electrode (RDE) measurements suggested that a higher current density was achieved over PN-NCNT than that on PN-CNT catalyst, which can be attributed to formation of the larger amount of N8− on NCNT. Kinetic study suggested a four-electron pathway mechanism over PN-NCNT. Moreover, it showed long stability and good methanol tolerance, which indicates its great potential application. This work provides insights on designing and synthesizing non-traditional metal-free catalysts for ORR in fuel cells.
Infection and Activation of B Cells by Theiler’s Murine Encephalomyelitis Virus (TMEV) Leads to Autoantibody Production in an Infectious Model of Multiple Sclerosis
Authors: Jin Young-Hee , Kim Charles X. , Huang Jocelin , Kim Byung S.
Published: July 2020

Theiler’s murine encephalomyelitis virus (TMEV) induces immune-mediated inflammatory demyelinating disease in susceptible mice that is similar to human multiple sclerosis (MS). In light of anti-CD20 therapies for MS, the susceptibility of B cells to TMEV infection is particularly important. In our study, direct viral exposure to macrophages and lymphocytes resulted in viral replication and cellular stimulation in the order of DCs, macrophages, B cells, and T cells. Notably, B cells produced viral proteins and expressed elevated levels of CD69, an activation marker. Similarly, the expression of major histocompatibility complex class II and costimulatory molecules in B cells was upregulated. Moreover, TMEV-infected B cells showed elevated levels of antigen-presenting function and antibody production. TMEV infection appeared to polyclonally activate B cells to produce autoantibodies and further T cell stimulation. Thus, the viral infection might potentially affect the outcome of autoimmune diseases, and/or the development of other chronic infections, including the protection and/or pathogenesis of TMEV-induced demyelinating disease.
Biological Properties of JNK3 and Its Function in Neurons, Astrocytes, Pancreatic β-Cells and Cardiovascular Cells
Authors: Nakano Rei , Nakayama Tomohiro , Sugiya Hiroshi
Published: July 2020

JNK is a protein kinase, which induces transactivation of c-jun. The three isoforms of JNK, JNK1, JNK2, and JNK3, are encoded by three distinct genes. JNK1 and JNK2 are expressed ubiquitously throughout the body. By contrast, the expression of JNK3 is limited and observed mainly in the brain, heart, and testes. Concerning the biological properties of JNKs, the contribution of upstream regulators and scaffold proteins plays an important role in the activation of JNKs. Since JNK signaling has been described as a form of stress-response signaling, the contribution of JNK3 to pathophysiological events, such as stress response or cell death including apoptosis, has been well studied. However, JNK3 also regulates the physiological functions of neurons and non-neuronal cells, such as development, regeneration, and differentiation/reprogramming. In this review, we shed light on the physiological functions of JNK3. In addition, we summarize recent advances in the knowledge regarding interactions between JNK3 and cellular reprogramming.
In-Depth Analysis Reveals Production of Circular RNAs from Non-Coding Sequences
Authors: Robic Annie , Demars Julie , Kühn Christa
Published: July 2020

The sequencing of total RNA depleted for ribosomal sequences remains the method of choice for the study of circRNAs. Our objective was to characterize non-canonical circRNAs, namely not originating from back splicing and circRNA produced by non-coding genes. To this end, we analyzed a dataset from porcine testis known to contain about 100 intron-derived circRNAs. Labelling reads containing a circular junction and originating from back splicing provided information on the very small contribution of long non-coding genes to the production of canonical circRNAs. Analyses of the other reads revealed two origins for non-canonical circRNAs: (1) Intronic sequences for lariat-derived intronic circRNAs and intron circles, (2) Mono-exonic genes (mostly non-coding) for either a new type of circRNA (including only part of the exon: sub-exonic circRNAs) or, even more rarely, mono-exonic canonical circRNAs. The most complex set of sub-exonic circRNAs was produced by RNase_MRP (ribozyme RNA). We specifically investigated the intronic circRNA of ATXN2L, which is probably an independently transcribed sisRNA (stable intronic sequence RNA). We may be witnessing the emergence of a new non-coding gene in the porcine genome. Our results are evidence that most non-canonical circRNAs originate from non-coding sequences.
Phage-Based Sensors in Medicine: A Review
Authors: Machera Sebastian J. , Niedziółka-Jönsson Joanna , Szot-Karpińska Katarzyna
Published: August 2020

Bacteriophages are interesting entities on the border of biology and chemistry. In nature, they are bacteria parasites, while, after genetic manipulation, they gain new properties, e.g., selectively binding proteins. Owing to this, they may be applied as recognition elements in biosensors. Combining bacteriophages with different transducers can then result in the development of innovative sensor designs that may revolutionize bioanalytics and improve the quality of medical services. Therefore, here, we review the use of bacteriophages, or peptides from bacteriophages, as new sensing elements for the recognition of biomarkers and the construction of the highly effective diagnostics tools.
Surface Imprinted Layer of Cypermethrin upon Au Nanoparticle as a Specific and Selective Coating for the Detection of Template Pesticide Molecules
Authors: Sitjar Jaya , Hou Ying-Chen , Liao Jiunn-Der , Lee Han , Xu Hong-Zheng , Fu Wei-En , Chen Guo Dung
Published: August 2020

The detection of specific pesticides on food products is essential as these substances pose health risks due to their toxicity. The use of surface-enhanced Raman spectroscopy (SERS) takes advantage of the straightforward technique to obtain fingerprint spectra of target analytes. In this study, SERS-active substrates are made using Au nanoparticles (NPs) coated with a layer of polymer and followed by imprinting with a pesticide–Cypermethrin, as a molecularly imprinted polymer (MIP). Cypermethrin was eventually removed and formed as template cavities, then denoted as Au NP/MIP, to capture the analogous molecules. The captured molecules situated in-between the areas of high electromagnetic field formed by plasmonic Au NPs result in an effect of SERS. The formation of Au NP/MIP was, respectively, studied through morphological analysis using transmission electron microscopy (TEM) and compositional analysis using X-ray photoelectron spectroscopy (XPS). Two relatively similar pesticides, Cypermethrin and Permethrin, were used as analytes. The results showed that Au NP/MIP was competent to detect both similar molecules despite the imprint being made only by Cypermethrin. Nevertheless, Au NP/MIP has a limited number of imprinted cavities that result in sensing only low concentrations of a pesticide solution. Au NP/MIP is thus a specific design for detecting analogous molecules similar to its template structure.
Foam Formation by Compression/Decompression Cycle of Soft Porous Media
Authors: Johnson Phillip , Vaccaro Mauro , Starov Victor , Trybala Anna
Published: August 2020

A theory of the amount of foam produced by compression/decompression cycles of a soft porous media is developed. The amount of foam produced was found to be dependent on both the amount of surfactant within the media and the minimum separation between the plates of the compression device. The latter is determined by the mechanical properties of the soft media. The theory also shows the importance of the decompression of the media as this is the mechanism of where the air penetrates into the soft porous material. The accumulated air is used during the compression stage for foam formation. The theoretically predicted values of foam mass are found to have good agreement with experimental observations, which validates the theory predictions. The theory also predicts independence of the foam produced in terms of the frequency of compression/decompression cycles, which agrees with our experimental observations.
Atomic Layer Deposition of Superconducting CuO Thin Films on Three-Dimensional Substrates
Authors: Tamm Aile , Tarre Aivar , Verchenko Valeriy , Seemen Helina , Stern Raivo
Published: July 2020

In previous decades, investigation of superconductors was aimed either at finding materials with higher critical temperatures or at discovering nontypical superconducting behavior. Here, we present the cupric (CuO) thin films, which were synthesized by atomic layer deposition by using a metal-organic precursor, copper (II)-bis-(-dimethylamino-2-propoxide), and ozone as an oxidizer. The deposition process was optimized by employing a quartz crystal monitoring, and the contact between the deposited films and planar and three-dimensional SiO2/Si substrates was examined by scanning electron microscopy with a focused ion beam module. Phase and elemental composition were analyzed by X-ray diffraction and X-ray fluorescence. Two-probe electrical resistivity measurements revealed a resistivity drop below the critical temperature of 4 K, which may indicate low-temperature superconductivity of the CuO thin films.
Synthesis, Crystal Structure and Solid State Transformation of 1,2-Bis[(1-methyl-1H-imidazole-2-yl)thio]ethane
Authors: Štefan Leo , Matković-Čalogović Dubravka , Filić Darko , Dumić Miljenko
Published: August 2020

The spontaneous S-alkylation of the thyreostatic drug methimazole (1-methyl-1,3-dihydro-1H-imidazole-2-thione, 1) with 1,2-dichloroethane at room temperature, in dark or light conditions, led to the formation of its related substance 1,2-bis[(1-methyl-1H-imidazole-2-yl)thio]ethane, C10H14N4S2 (2a), primarily isolated in the form of dihydrochloride tetrahydrate [C10H16N4S2]Cl2·4(H2O) (2b), which crystallized in the monoclinic P21/c space group. Neutralization of 2b, followed by crystallization from the acetone/water mixture, produced dihydrate C10H14N4S2·2(H2O) (2c), which crystallized in the trigonal R-3 space group. Six water molecules in 2c are H-bonded mutually and to the nitrogen atoms of six molecules of 2a. DSC and TGA showed that 2c melts at 65 °C and loses water up to 120 °C. By cooling to room temperature, anhydrous 2a was obtained. Single crystals of 2a that are suitable for X-ray structure analysis were obtained by neutralization of 2b, followed by crystallization from dry dichloromethane. Anhydrous 2a crystallizes in the monoclinic P21/c space group. The dehydration of 2c led to the formation of the anhydrous product 2a, which is identical to the one obtained by crystallization, as was found by complementary solid-state techniques. No intermediate monohydrate or hemihydrate phases were detected. Powder diffraction showed the same pattern of 2c via both preparation procedures. The structures of all the forms were elucidated by spectroscopy, microscopy and thermal methods and confirmed by single crystal X-ray analysis.
Cyanobacteria Phylogenetic Studies Reveal Evidence for Polyphyletic Genera from Thermal and Freshwater Habitats
Authors: Cordeiro Rita , Luz Rúben , Vasconcelos Vitor , Gonçalves Vítor , Fonseca Amélia
Published: July 2020

Cyanobacteria are among the most diverse morphological microorganisms that inhabit a great variety of habitats. Their presence in the Azores, a volcanic archipelago of nine islands in the middle of the North Atlantic Ocean, has already been reported. However, due to the high diversity of cyanobacteria habitats, their biodiversity is still understudied, mainly in extreme environments. To address this, a total of 156 cyanobacteria strains from Azores lakes, streams, thermal and terrestrial habitats were isolated. Identification was made based on a polyphasic approach using classical taxonomy (morphological characteristics and environmental data) and phylogeny among 81 strains assessed by maximum likelihood and Bayesian analysis of 16S rDNA partial sequences. The 156 isolates showed a high genera diversity (38) belonging to the orders Chroococcales, Nostocales, Oscillatoriales, and Synechococcales. Eleven new genera for the Azores habitats are here reported, reinforcing that cyanobacteria biodiversity in these islands is still much understudied. Phylogenetic analysis showed 14 clusters associated with these cyanobacteria orders, with evidence for six new genera and valuable information towards Microchaete/Coleospermum taxonomic revision that better reflects species environmental distribution. These results emphasize the need for cyanobacteria taxonomy revisions, through polyphasic studies, mainly in Synechococcales order and in the Microchaete/Coleospermum, Nostoc, and Anabaena genera.
Identification of Stray Gassing of Inhibited and Uninhibited Mineral Oils in Transformers
Authors: Duval Michel , Heizmann Thomas
Published: July 2020

The aim and contribution of this paper is to identify with Duval Pentagon 2 the stray gassing (SG) patterns of inhibited and uninhibited mineral oils in transformers in service and in the well-established laboratory SG tests of CIGRE and ASTM, so that SG in transformers can be easily distinguished from the other types of faults occurring in them. The SG test of IEC 60296-2020 is inadequate or much less effective for that purpose.
Role of Species and Planting Configuration on Transpiration and Microclimate for Urban Trees
Authors: Zhao Dan , Lei Quanhuan , Shi Yajie , Wang Mengdi , Chen Sibo , Shah Kamran , Ji Wenli
Published: July 2020

Research Highlights: To demonstrate the effectiveness of configuration modes and tree types in regulating local microclimate. Background and Objectives: Urban trees play an essential role in reducing the city’s heat load. However, the influence of urban trees with different configurations on the urban thermal environment has not received enough attention. Herein we show how spatial arrangement and foliage longevity, deciduous versus evergreen, affect transpiration and the urban microclimate. Materials and Methods: We analyzed the differences between physiological parameters (transpiration rate, stomatal conductance) and meteorological parameters (air temperature, relative humidity, vapor pressure deficit) of 10 different species of urban trees (five evergreen and five deciduous tree species), each of which had been planted in three configuration modes in a park and the campus green space in Xi’an. By manipulating physiological parameters, crown morphology, and plant configurations, we explored how local urban microclimate could be altered. Results: (1) Microclimate regulation capacity: group planting (GP) > linear planting (LP) > individual planting (IP). (2) Deciduous trees (DT) regulated microclimate better than evergreen trees (ET). Significant differences between all planting configurations during 8 to 16 h were noted for evergreen trees whereas for deciduous trees, all measurement times were significantly different. (3) Transpiration characteristics: GP > LP > IP. The transpiration rate (E) and stomatal conductance (Gs) of GP were the highest. Total daily transpiration was ranked as group planting of deciduous (DGP) > linear planting of deciduous (DLP) > group planting of evergreen (EGP) > linear planting of evergreen (ELP) > isolated planting of deciduous (DIP) > isolated planting of evergreen (EIP). (4) The microclimate effects of different tree species and configuration modes were positively correlated with E, Gs, and three dimensional green quantity (3DGQ), but weakly correlated with vapor pressure deficit (VpdL). (5) A microclimate regulation capability model of urban trees was developed. E, Gs, and 3DGQ could explain 93% variation of cooling effect, while E, Gs, VpdL, and 3DGQ could explain 85% variation of humidifying effect. Conclusions: This study demonstrated that the urban heat island could be mitigated by selecting deciduous broadleaf tree species and planting them in groups.
Genome Cytosine Methylation May Affect Growth and Wood Property Traits in Populations of Populus tomentosa
Authors: Ma Kaifeng , Song Yuepeng , Ci Dong , Zhou Daling , Tian Min , Zhang Deqiang
Published: July 2020

Growth and wood formation are crucial and complex biological processes during tree development. These biological regulatory processes are presumed to be controlled by DNA methylation. However, there is little direct evidence to show that genes taking part in wood regulation are affected by cytosine methylation, resulting in phenotypic variations. Here, we detected epimarkers using a methylation-sensitive amplification polymorphism (MSAP) method and performed epimarker–trait association analysis on the basis of nine growth and wood property traits within populations of 432 genotypes of Populus tomentosa. Tree height was positively correlated with relative full-methylation level, and 1101 out of 2393 polymorphic epimarkers were associated with phenotypic traits, explaining 1.1–7.8% of the phenotypic variation. In total, 116 epimarkers were successfully sequenced, and 96 out of these sequences were linked to putative genes. Among them, 13 candidate genes were randomly selected for verification using quantitative real-time PCR (qRT-PCR), and it also showed the expression of nine putative genes of PtCYP450, PtCpn60, PtPME, PtSCP, PtGH, PtMYB, PtWRKY, PtSTP, and PtABC were negatively correlated with DNA methylation level. Therefore, it suggested that changes in DNA methylation might contribute to regulating tree growth and wood property traits.
Landslide Detection and Susceptibility Modeling on Cameron Highlands (Malaysia): A Comparison between Random Forest, Logistic Regression and Logistic Model Tree Algorithms
Authors: Nhu Viet-Ha , Mohammadi Ayub , Shahabi Himan , Ahmad Baharin Bin , Al-Ansari Nadhir , Shirzadi Ataollah , Geertsema Marten , R. Kress Victoria , Karimzadeh Sadra , Valizadeh Kamran Khalil , Chen Wei , Nguyen Hoang
Published: July 2020

We used remote sensing techniques and machine learning to detect and map landslides, and landslide susceptibility in the Cameron Highlands, Malaysia. We located 152 landslides using a combination of interferometry synthetic aperture radar (InSAR), Google Earth (GE), and field surveys. Of the total slide locations, 80% (122 landslides) were utilized for training the selected algorithms, and the remaining 20% (30 landslides) were applied for validation purposes. We employed 17 conditioning factors, including slope angle, aspect, elevation, curvature, profile curvature, stream power index (SPI), topographic wetness index (TWI), lithology, soil type, land cover, normalized difference vegetation index (NDVI), distance to river, distance to fault, distance to road, river density, fault density, and road density, which were produced from satellite imageries, geological map, soil maps, and a digital elevation model (DEM). We used these factors to produce landslide susceptibility maps using logistic regression (LR), logistic model tree (LMT), and random forest (RF) models. To assess prediction accuracy of the models we employed the following statistical measures: negative predictive value (NPV), sensitivity, positive predictive value (PPV), specificity, root-mean-squared error (RMSE), accuracy, and area under the receiver operating characteristic (ROC) curve (AUC). Our results indicated that the AUC was 92%, 90%, and 88% for the LMT, LR, and RF algorithms, respectively. To assess model performance, we also applied non-parametric statistical tests of Friedman and Wilcoxon, where the results revealed that there were no practical differences among the used models in the study area. While landslide mapping in tropical environment such as Cameron Highlands remains difficult, the remote sensing (RS) along with machine learning techniques, such as the LMT model, show promise for landslide susceptibility mapping in the study area.
Scale-Resolving Simulation of a Propane-Fuelled Industrial Gas Turbine Combustor Using Finite-Rate Tabulated Chemistry
Authors: Zhang Kai , Ghobadian Ali , Nouri Jamshid M.
Published: July 2020

The scale-resolving simulation of a practical gas turbine combustor is performed using a partially premixed finite-rate chemistry combustion model. The combustion model assumes finite-rate chemistry by limiting the chemical reaction rate with flame speed. A comparison of the numerical results with the experimental temperature and species mole fraction clearly showed the superiority of the shear stress transport, K-omega, scale adaptive turbulence model (SSTKWSAS). The model outperforms large eddy simulation (LES) in the primary region of the combustor, probably for two reasons. First, the lower amount of mesh employed in the simulation for the industrial-size combustor does not fit the LES’s explicit mesh size dependency requirement, while it is sufficient for the SSTKWSAS simulation. Second, coupling the finite-rate chemistry method with the SSTKWSAS model provides a more reasonable rate of chemical reaction than that predicted by the fast chemistry method used in LES simulation. Other than comparing with the LES data available in the literature, the SSTKWSAS-predicted result is also compared comprehensively with that obtained from the model based on the unsteady Reynolds-averaged Navier–Stokes (URANS) simulation approach. The superiority of the SSTKWSAS model in resolving large eddies is highlighted. Overall, the present study emphasizes the effectiveness and efficiency of coupling a partially premixed combustion model with a scale-resolving simulation method in predicting a swirl-stabilized, multi-jets turbulent flame in a practical, complex gas turbine combustor configuration.
Lagrangian Statistics of Heat Transfer in Homogeneous Turbulence Driven by Boussinesq Convection
Authors: Pratt Jane , Busse Angela , Müller Wolf-Christian
Published: August 2020

The movement of heat in a convecting system is typically described by the nondimensional Nusselt number, which involves an average over both space and time. In direct numerical simulations of turbulent flows, there is considerable variation in the contributions to the Nusselt number, both because of local spatial variations due to plumes and because of intermittency in time. We develop a statistical approach to more completely describe the structure of heat transfer, using an exit-distance extracted from Lagrangian tracer particles, which we call the Lagrangian heat structure. In a comparison between simulations of homogeneous turbulence driven by Boussinesq convection, the Lagrangian heat structure reveals significant non-Gaussian character, as well as a clear trend with Prandtl number and Rayleigh number. This has encouraging implications for simulations performed with the goal of understanding turbulent convection in natural settings such as Earth’s atmosphere and oceans, as well as planetary and stellar dynamos.
Nutritional Features and Bread-Making Performance of Wholewheat: Does the Milling System Matter?
Authors: Pagani Maria Ambrogina , Giordano Debora , Cardone Gaetano , Pasqualone Antonella , Casiraghi Maria Cristina , Erba Daniela , Blandino Massimo , Marti Alessandra
Published: August 2020

Despite the interest in stone-milling, there is no information on the potential advantages of using the resultant wholegrain flour (WF) in bread-making. Consequently, nutritional and technological properties of WFs obtained by both stone- (SWF) and roller-milling (RWF) were assessed on four wheat samples, differing in grain hardness and pigment richness. Regardless of the type of wheat, stone-milling led to WFs with a high number of particles ranging in size from 315 to 710 μm), whereas RWFs showed a bimodal distribution with large (>1000 μm) and fine (<250 μm) particles. On average, the milling system did not affect the proximate composition and the bioactive features of WFs. The gluten aggregation kinetics resulted in similar trends for all SWFs, with indices higher than for RWFs. The effect of milling on dough properties (i.e., mixing and leavening) was sample dependent. Overall, SWFs produced more gas, resulting in bread with higher specific volume. Bread crumb from SWF had higher lutein content in the wheat cv rich in xanthophylls, while bread from RWF of the blue-grained cv had a moderate but significantly higher content in esterified phenolic acids and total anthocyanins. In conclusion, there was no relevant advantage in using stone- as opposed to roller-milling (and vice versa).
Estimation of Dietary Exposure to Contaminants Transferred from the Packaging in Fatty Dry Foods Based on Cereals
Authors: Lestido-Cardama Antía , Rodríguez Bernaldo de Quirós Ana , Bustos Juana , Lomo M. Luisa , Paseiro Losada Perfecto , Sendón Raquel
Published: August 2020

Food packaging has received special attention from the food safety standpoint since it could be a potential source of contamination through the migration of chemical substances from the packaging material into food. The assessment of the exposure through the diet to these contaminants from food packaging is necessary. In this work, an estimation of dietary exposure of the young Spanish population (1–17 years) to target chemicals from packaging for fatty dried foods based on cereals was assessed. For this purpose, a gas chromatography coupled to mass spectrometry (GC–MS) method was developed for screening of volatile and semivolatile compounds, potential migrants from the packaging. Then, this technique was used to quantify 8 target analytes, which were previously identified in the packaging (including phthalates, acetyl tributyl citrate (ATBC), butylated hydroxytoluene (BHT) and octocrylene), in composite food samples of fatty cereals prepared according to the consumption data for different age groups. Among the phthalates, exposure to diethyl phthalate (DEP) was the highest for the three groups considered (0.0761–0.545 µg/kg body weight/day), followed by bis(2-ethylhxyl)phathalate (DEHP), while the lowest mean intake was found for di-n-octyl phathalate (DNOP; 0.00463–0.0209 µg/kg body weight/day). The estimated dietary exposures did not exceed for any of the analytes the corresponding established tolerable daily intakes.
Quality of Chicken Fat by-Products: Lipid Profile and Colour Properties
Authors: Peña-Saldarriaga Lina María , Fernández-López Juana , Pérez-Alvarez José Angel
Published: August 2020

The growth in the production and consumption of chicken meat and related products is responsible for the formation a large number of by-products. Among these, abdominal and gizzard fat is usually considered as waste and thus is discarded, creating an environmental problem. This work aims to characterize chicken fat by-products, evaluating their lipid profile and colour properties for their potential use as fat sources in meat products in substitution of traditionally used fats. In addition, the role of farm location, keeping the feeding and other farmer routines fixed, in the lipid profile was also evaluated. “Parrilleros” Colombian chickens from three farms located in various geographical zones of the Antioquia region were selected. After slaughtering, abdominal and gizzard fat was obtained. Lipid profile was evaluated by gas chromatography and the CIELAB colour properties were assessed. The production results and the lipid profile of chicken fat by-products (abdominal and gizzard fat) was similar in the three farms studied, which is important for their potential application as fat source in the formulation of meat products. The predominant fatty acids were oleic, palmitic and linoleic acids, showing a higher amount of unsaturated fatty acids than the fat sources traditionally used for this purpose. Valorization of chicken by-products by the use of abdominal and gizzard fat as fat source in chicken meat products formulation could be a feasible alternative contributing to the poultry sector sustainability.
Growth Potential of Listeria monocytogenes in Three Different Salmon Products
Authors: Eicher Corinne , Ruiz Subira Andres , Corti Sabrina , Meusburger Arnulf , Stephan Roger , Guldimann Claudia
Published: August 2020

Cold smoked salmon and sushi salmon have been implicated in outbreaks of listeriosis. We performed challenge tests and a durability study with Listeria monocytogenes on different salmon products to determine the growth potential of this important food-borne pathogen. Data from the challenge test showed a significant growth potential of L. monocytogenes on all of the tested salmon products, with faster growth in sushi salmon than in cold smoked salmon. In identical products that were naturally contaminated at low levels, the durability study did not confirm a high growth potential, possibly due to interactions with competing microflora. The injection of sodium lactate (NaL) at a high concentration (30%) into cold smoked salmon significantly reduced the growth potential of L. monocytogenes. In addition to good manufacturing practices, the injection of higher concentrations of NaL may therefore be a useful additional hurdle to prevent growth of L. monocytogenes to high numbers in the tested salmon products.
Composting Settler Colonial Distortions: Cultivating Critical Land-Based Family History
Authors: French Kristen B. , Sanchez Amy , Ullom Eddy
Published: August 2020

A collective of three intergenerational and intersectional educators engage in anti-colonial and/or decolonial processes of composting colonial distortions through Land-based conceptualizations of Critical Family History. Engaging in spiral discourse through Critical Personal Narratives, the authors theorize critical family history, Land-based learning, and Indigenous decolonial and anti-settler colonial frameworks. Using a process of unsettling reflexivity to analyze and interrupt settler colonial logics, the authors share their storied journeys, lessons learned and limitations for the cultivation of Critical Land-based Family History.
Association between Platelet-Specific Collagen Receptor Glycoprotein 6 Gene Variants, Selected Biomarkers, and Recurrent Pregnancy Loss in Korean Women
Authors: An Hui Jeong , Ahn Eun Hee , Kim Jung Oh , Ryu Chang Soo , Park Han Sung , Cho Sung Hwan , Kim Ji Hyang , Lee Woo Sik , Lee Jung Ryeol , Kim Young Ran , Kim Nam Keun
Published: July 2020

This paper investigates whether glycoprotein 6 (GP6) gene polymorphisms are a risk factor for recurrent pregnancy loss (RPL) in Korean women. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism and real-time polymerase chain reaction amplification. We identified five polymorphisms in the GP6 gene: rs1654410 T>C, rs1671153 T>G, rs1654419 G>A, rs12610286 A>G, and rs1654431 G>A. GP6 rs1654410 CC was associated with decreased RPL risk (adjusted odds ratio = 0.292, 95% confidence interval = 0.105–0.815, p = 0.019), and recessive genotypes were also significantly associated with decreased RPL risk (adjusted odds ratio = 0.348, 95% confidence interval = 0.128−0.944, p = 0.038). GP6 rs1654419 GA was associated with decreased RPL risk (adjusted odds ratio = 0.607, 95% confidence interval = 0.375-0.982, p = 0.042), and dominant genotypes were significantly associated with decreased RPL risk (adjusted odds ratio = 0.563, 95% confidence interval = 0.358−0.885, p = 0.013). Altogether, the genotype frequencies of GP6 rs1654410 T>C and GP6 rs1654419 G>A were significantly different between RPL patients and control participants. Therefore, although GP6 polymorphisms may be useful as biomarkers of RPL, additional studies with heterogeneous cohorts are required to better understand the influence of GP6 and assess its performance as a biomarker.
Analysis of the Possible Reactivation of the Krbavčići Landslide in Northern Istria, Croatia
Authors: Vivoda Prodan Martina , Arbanas Željko
Published: August 2020

The Krbavčići landslide occurred in January 1979 near the town of Buzet, Croatia, after a long period of heavy rainfall. It is located in Northern Istria in the area built of flysch rock mass where numerous mass movements in the past and recent history have been recorded. A flysch rock mass is highly susceptible to weathering, which leads to material disintegration, changes in geotechnical properties, and shear strength decrease, finally resulting in instability processes in flysch slopes. This paper describes existing information about the Krbavčići landslide occurrence, laboratory testing of siltstone samples from a flysch rock mass, and numerical slope stability analyses of a possible landslide reactivation caused by possible long rainy periods and further weathering of the flysch rock mass. Slope stability analysis using the Rocscience, Slide software, as well as landslide numerical simulations using the LS-Rapid simulation software were performed on the basis of the digital elevation model (DEM) and laboratory test results of siltstones with different weathering grades. A DEM of the Krbavčići landslide was obtained on the basis of the unmanned aerial vehicle (UAV) survey conducted in March 2016. The residual shear strength of siltstones to predict a reactivation of landslides is of highest importance and was determined by ring shear and direct shear tests on siltstone samples with different weathering grades. The results of the numerical simulations show that an increase of the groundwater level in the landslide body in combination with the further weathering of the flysch rock material at the sliding surface would have the main influence on a possible landslide reactivation and the further development of the landslide displacement.
Linking Siberian Traps LIP Emplacement and End-Permian Mass Extinction: Evidence from Magnetic Stratigraphy of the Maymecha-Kotuy Volcanic Section
Authors: Latyshev Anton V. , Fetisova Anna M. , Veselovskiy Roman V.
Published: August 2020

The Siberian Traps Large Igneous Provinces (LIP) emplacement is considered as one of possible triggers for the end-Permian global biotic crisis. However, relative timing of the onset of extinction and the main phase of the magmatic activity are not yet accurately constrained. We present the detailed paleomagnetic data for the thickest composite section of the Siberian Traps volcanics, located in the Maymecha-Kotuy region. The major part of the Maymecha-Kotuy section erupted in the beginning of Early Triassic period and postdate came the onset of the biotic crisis. However, the initial pulse of volcanic activity in this region took place at the end of the Permian period, and likely preceded the extinction event, being nearly coeval to the lowest part of tuff-lava sequence of Norilsk. The suggested correlation scheme of volcanic sections from different regions of the Siberian platform shows that explosive and extrusive events foregoing the onset of extinction can be identified in almost all regions of the Siberian Traps LIP. Finally, we estimate the total duration of magmatic activity in the Maymecha-Kotuy region as ~2 Myr and assume that this lasted after the termination of eruptions in other parts of the Siberian platform.
Modeling Internal Movement of Children Born in Hong Kong to Nonlocal Mothers
Authors: Yip Paul , Soleymani Mehdi , Wat Kam Pui , Pinkney Edward , Lam Kwok Fai
Published: July 2020

In Hong Kong, approximately 300,000 children were born to Mainland China couples in the period 1991–2012. According to Basic Law, the mini constitution of Hong Kong Special Administrative Region (SAR) government, these parents do not have residence rights, but their children do. As a result, most of these children have returned to Mainland China with their parents. An important consideration for policymakers is how many of these children (who are now adults in some cases) will return to Hong Kong for good, and when, as this will have a significant impact on social service provision, especially in the education sector, where it will be necessary to ensure there is capacity to meet the additional demand. Prior survey results conducted by the government suggested that more than 50% of these children would return to Hong Kong before age six. It is important to be able to provide a timely projection of the demand into the future. Here, we make use of the immigration records on the actual movement of these children and propose a Markov chain model to estimate their return rates in the future. Our results show that only about 25% of these children would return rather than 50% estimated by the survey. We also find that parents with better educational attainment levels are associated with lower return rates of their children. Timely and relevant social and public policies are needed to prepare for their return to minimize disruption to the local population and promote social harmony for the whole community.