bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimising Embodied Energy and Thermal Performance of Thermal Insulation in Building Envelopes via an Automated Building Information Modelling (BIM) Tool
Authors: Chen Zixuan , Hammad Ahmed , Kamardeen Imriyas , Akbarnezhad Ali
Published: November 2020

Insulation systems for the floor, roof, and external walls play a prominent role in providing a thermal barrier for the building envelope. Design decisions made for the insulation material type and thickness can alleviate potential impacts on the embodied energy and improve the building thermal performance. This design problem is often addressed using a building information modelling (BIM)-integrated optimisation approach. However, one major weakness that lies in the current studies is that BIM is merely used as the source for design parameters input. This study proposes a BIM-based envelope insulation optimisation design tool using a common software Revit and its extension Dynamo to find the trade-off between the total embodied energy of the insulation system and the thermal performance of the envelope by considering the material type and thickness. In addition, the tool also permits data visualisation in a BIM environment, and automates subsequent material library mapping and instantiates the optimal insulation designs. The framework is tested on a case study based in Sydney, Australia. By analysing sample designs from the Pareto front, it is found that slight improvement in the thermal performance (1.3399 to 1.2112 GJ/m2) would cause the embodied energy to increase by more than 50 times.
Lactobacillus Attenuate the Progression of Pancreatic Cancer Promoted by Porphyromonas Gingivalis in K-rasG12D Transgenic Mice
Authors: Chen Shan-Ming , Hsu Li-Jin , Lee Hsiang-Lin , Lin Ching-Pin , Huang Szu-Wei , Lai Caucasus Jun-Lin , Lin Chia-Wei , Chen Wan-Ting , Chen Yu-Jen , Lin Yu-Chien , Yang Chi-Chieh , Jan Ming-Shiou
Published: November 2020

Accumulating evidence suggests that there is a link between the host microbiome and pancreatic carcinogenesis, and that Porphyromonas gingivalis (P. gingivalis) increases the risk of developing pancreatic cancer. The aim of the current study was to clarify the role of P. gingivalis in the pathogenesis of pancreatic cancer and the potential immune modulatory effects of probiotics. The six-week-old LSL-K-rasG12D; Pdx-1-cre (KC) mice smeared P. gingivalis on the gums, causing pancreatic intraepithelial neoplasia (PanIN) after four weeks to be similar to the extent of lesions in untreated KC mice at 24 weeks. The oral inoculation of P. gingivalis of six-week-old LSL-K-rasG12D; Pdx-1-cre (KC) mice caused significantly pancreatic intraepithelial neoplasia (PanIN) after treatment four weeks is similar to the extent of lesions in untreated KC mice at 24 weeks. The pancreas weights of P. gingivalis plus probiotic-treated mice were significantly lower than the mice treated with P. gingivalis alone (P = 0.0028). The histological expressions of Snail-1, ZEB-1, collagen fibers, Galectin-3, and PD-L1 staining in the pancreas were also notably lower. In addition, probiotic administration reduced the histological expression of Smad3 and phosphorylated Smad3 in P. gingivalis treated KC mice. We demonstrated that oral exposure to P. gingivalis can accelerate the development of PanIN lesions. Probiotics are likely to have a beneficial effect by reducing cancer cell proliferation and viability, inhibiting PanIN progression, and cancer cell metastasis (Epithelial–mesenchymal transition, EMT). The transforming growth factor-β signaling pathway may be involved in the tumor suppressive effects of probiotics.
Correlation between Intraprostatic PSMA Uptake and MRI PI-RADS of [68Ga]Ga-PSMA-11 PET/MRI in Patients with Prostate Cancer: Comparison of PI-RADS Version 2.0 and PI-RADS Version 2.1
Authors: Zhao Jing , Mangarova Dilyana B. , Brangsch Julia , Kader Avan , Hamm Bernd , Brenner Winfried , Makowski Marcus R.
Published: November 2020

Purpose: We aimed to evaluate the correlation between PSMA uptake and magnetic resonance imaging (MRI) PI-RADS of simultaneous [68Ga]Ga-PSMA-11 PET/MRI regarding PI-RADS version 2.0 and 2.1 respectively and compared the difference between these two versions. Materials and Methods: We retrospectively analyzed a total of forty-six patients with biopsy-proven prostate cancer who underwent simultaneous [68Ga]Ga-PSMA-11 PET/MRI. We classified the lesions regarding PI-RADS version 2.0 and 2.1, peripheral zone (PZ), and transitional zone (TZ), respectively. Based on regions of interest (ROI), standardized uptake values maximum (SUVmax), and corresponding lesion-to-background ratios (LBR) of SUVmax of each category, PI-RADS score 1 to 5, were measured. A comparison between PI-RADS version 2.0 and PI-RADS version 2.1 was performed. Results: A total of 215 focal prostate lesions were analyzed, including two subgroups, 125 TZ and 90 PZ. Data are reported as median and interquartile range (IQR). Regarding PI-RADS version 2.1, TZ SUVmax of each category were 1.5 (0.5, 1.9), 1.9 (0.8, 2.3), 3.3 (2.1, 4.6), 4.2 (3.1, 5.7), 7.3 (5.2, 9.7). PZ SUVmax of each category were 1.0 (0.8, 1.6), 2.5 (1.5, 3.2), 3.3 (1.9, 4.5), 4.3 (3.0, 5.4), 7.4 (5.0, 9.3). Regarding the inter-reader agreement of the overall PI-RADS assessment category, the kappa value was 0.723 for version 2.0 and 0.853 for version 2.1. Conclusion: Revisions of PI-RADS version 2.1 results in variations in lesions classification. Lesions with the PI-RADS category of 3, 4, and 5 present relatively higher intraprostatic PSMA uptake, while lesions with the PI-RADS category of 1 and 2 present relatively lower and similar uptake. Version 2.1 has higher inter-reader reproducibility than version 2.0.
SAHA Overcomes 5-FU Resistance in IFIT2-Depleted Oral Squamous Cell Carcinoma Cells
Authors: Regmi Prabha , Lai Kuo-Chu , Liu Chung-Ji , Lee Te-Chang
Published: November 2020

Interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) is a member of the interferon-stimulated gene family that contains tetratricopeptide repeats (TPRs), which mediate protein–protein interactions in various biological systems. We previously showed the depletion of IFIT2 enhanced cell migration and metastatic activity in oral squamous cell carcinoma (OSCC) cells via the activation of atypical PKC signaling. In this study, we found that IFIT2-knockdown cells displayed higher resistance to 5-fluorouracil (5-FU) than control cells. The comet assay and annexin V analysis showed decreased DNA damage and cell death in IFIT2-knockdown cells compared to control cells treated with 5-FU. Cell cycle progression was also perturbed by 5-FU treatment, with the accumulation of IFIT2-depleted cells in S phase in a time-dependent manner. We further observed the overexpression of thymidylate synthase (TS) and thymidine kinase (TK) in IFIT2-knockdown cells. Inhibition of TS alone or double inhibition of TS and TK1 using the siRNA technique increased susceptibility to 5-FU in IFIT2-knockdown cells. We further identified that suberanilohydroxamic acid (SAHA) treatment decreased the expression of TS in IFIT2-knockdown cells and demonstrated that pretreatment with SAHA sensitized IFIT2-knockdown cells to 5-FU in vitro and in vivo. In conclusion, IFIT2 knockdown enhances TS expression, which mediates 5-FU resistance, and SAHA pretreatment suppresses TS expression and hence sensitizes cells to 5-FU. SAHA will be an effective strategy for the treatment of OSCC patients with 5-FU resistance.
Epigenetic Inactivation of the Tumor Suppressor IRX1 Occurs Frequently in Lung Adenocarcinoma and Its Silencing Is Associated with Impaired Prognosis
Authors: Küster Miriam M. , Schneider Marc A. , Richter Antje M. , Richtmann Sarah , Winter Hauke , Kriegsmann Mark , Pullamsetti Soni S. , Stiewe Thorsten , Savai Rajkumar , Muley Thomas , Dammann Reinhard H.
Published: November 2020

Iroquois homeobox (IRX) encodes members of homeodomain containing genes which are involved in development and differentiation. Since it has been reported that the IRX1 gene is localized in a lung cancer susceptibility locus, the epigenetic regulation and function of IRX1 was investigated in lung carcinogenesis. We observed frequent hypermethylation of the IRX1 promoter in non-small cell lung cancer (NSCLC) compared to small cell lung cancer (SCLC). Aberrant IRX1 methylation was significantly correlated with reduced IRX1 expression. In normal lung samples, the IRX1 promoter showed lower median DNA methylation levels (<10%) compared to primary adenocarcinoma (ADC, 22%) and squamous cell carcinoma (SQCC, 14%). A significant hypermethylation and downregulation of IRX1 was detected in ADC and SQCC compared to matching normal lung samples (p < 0.0001). Low IRX1 expression was significantly correlated with impaired prognosis of ADC patients (p = 0.001). Reduced survival probability was also associated with higher IRX1 promoter methylation (p = 0.02). Inhibition of DNA methyltransferase (DNMT) activity reactivated IRX1 expression in human lung cancer cell lines. Induced DNMT3A and EZH2 expression was correlated with downregulation of IRX1. On the cellular level, IRX1 exhibits nuclear localization and expression of IRX1 induced fragmented nuclei in cancer cells. Localization of IRX1 and induction of aberrant nuclei were dependent on the presence of the homeobox of IRX1. By data mining, we showed that IRX1 is negatively correlated with oncogenic pathways and IRX1 expression induces the proapoptotic regulator BAX. In conclusion, we report that IRX1 expression is significantly associated with improved survival probability of ADC patients. IRX1 hypermethylation may serve as molecular biomarker for ADC diagnosis and prognosis. Our data suggest that IRX1 acts as an epigenetically regulated tumor suppressor in the pathogenesis of lung cancer.
Current Progresses and Challenges of Immunotherapy in Triple-Negative Breast Cancer
Authors: Mediratta Karan , El-Sahli Sara , D’Costa Vanessa , Wang Lisheng
Published: November 2020

With improved understanding of the immunogenicity of triple-negative breast cancer (TNBC), immunotherapy has emerged as a promising candidate to treat this lethal disease owing to the lack of specific targets and effective treatments. While immune checkpoint inhibition (ICI) has been effectively used in immunotherapy for several types of solid tumor, monotherapies targeting programmed death 1 (PD-1), its ligand PD-L1, or cytotoxic T lymphocyte-associated protein 4 (CTLA-4) have shown little efficacy for TNBC patients. Over the past few years, various therapeutic candidates have been reviewed, attempting to improve ICI efficacy on TNBC through combinatorial treatment. In this review, we describe the clinical limitations of ICI and illustrate candidates from an immunological, pharmacological, and metabolic perspective that may potentiate therapy to improve the outcomes of TNBC patients.
Exploiting GRK2 Inhibition as a Therapeutic Option in Experimental Cancer Treatment: Role of p53-Induced Mitochondrial Apoptosis
Authors: Gambardella Jessica , Fiordelisi Antonella , Santulli Gaetano , Ciccarelli Michele , Cerasuolo Federica Andrea , Sala Marina , Sommella Eduardo , Campiglia Pietro , Illario Maddalena , Iaccarino Guido , Sorriento Daniela
Published: November 2020

The involvement of GRK2 in cancer cell proliferation and its counter-regulation of p53 have been suggested in breast cancer even if the underlying mechanism has not yet been elucidated. Furthermore, the possibility to pharmacologically inhibit GRK2 to delay cancer cell proliferation has never been explored. We investigated this possibility by setting up a study that combined in vitro and in vivo models to underpin the crosstalk between GRK2 and p53. To reach this aim, we took advantage of the different expression of p53 in cell lines of thyroid cancer (BHT 101 expressing p53 and FRO cells, which are p53-null) in which we overexpressed or silenced GRK2. The pharmacological inhibition of GRK2 was achieved using the specific inhibitor KRX-C7. The in vivo study was performed in Balb/c nude mice, where we treated BHT-101 or FRO-derived tumors with KRX-C7. In our in vitro model, FRO cells were unaffected by GRK2 expression levels, whereas BHT-101 cells were sensitive, thus suggesting a role for p53. The regulation of p53 by GRK2 is due to phosphorylative events in Thr-55, which induce the degradation of p53. In BHT-101 cells, the pharmacologic inhibition of GRK2 by KRX-C7 increased p53 levels and activated apoptosis through the mitochondrial release of cytochrome c. These KRX-C7-mediated events were also confirmed in cancer allograft models in nude mice. In conclusion, our data showed that GRK2 counter-regulates p53 expression in cancer cells through a kinase-dependent activity. Our results further corroborate the anti-proliferative role of GRK2 inhibitors in p53-sensitive tumors and propose GRK2 as a therapeutic target in selected cancers.
Targeting the RhoGEF βPIX/COOL-1 in Glioblastoma: Proof of Concept Studies
Authors: Connor Kate , Murray David W. , Jarzabek Monika A. , Tran Nhan L. , White Kieron , Dicker Patrick , Sweeney Kieron J. , O’Halloran Philip J. , MacCarthy Brian , Shiels Liam P. , Lodi Francesca , Lambrechts Diether , Sarkaria Jann N. , Schiffelers Raymond M. , Symons Marc , Byrne Annette T.
Published: November 2020

Glioblastoma (GBM), a highly invasive and vascular malignancy is shown to rapidly develop resistance and evolve to a more invasive phenotype following bevacizumab (Bev) therapy. Rho Guanine Nucleotide Exchange Factor proteins (RhoGEFs) are mediators of key components in Bev resistance pathways, GBM and Bev-induced invasion. To identify GEFs with enhanced mRNA expression in the leading edge of GBM tumours, a cohort of GEFs was assessed using a clinical dataset. The GEF βPix/COOL-1 was identified, and the functional effect of gene depletion assessed using 3D-boyden chamber, proliferation, and colony formation assays in GBM cells. Anti-angiogenic effects were assessed in endothelial cells using tube formation and wound healing assays. In vivo effects of βPix/COOL-1-siRNA delivered via RGD-Nanoparticle in combination with Bev was studied in an invasive model of GBM. We found that siRNA-mediated knockdown of βPix/COOL-1 in vitro decreased cell invasion, proliferation and increased apoptosis in GBM cell lines. Moreover βPix/COOL-1 mediated endothelial cell migration in vitro. Mice treated with βPix/COOL-1 siRNA-loaded RGD-Nanoparticle and Bev demonstrated a trend towards improved median survival compared with Bev monotherapy. Our hypothesis generating study suggests that the RhoGEF βPix/COOL-1 may represent a target of vulnerability in GBM, in particular to improve Bev efficacy.
The Role of Plastic Reconstructive Surgery in Surgical Therapy of Soft Tissue Sarcomas
Authors: Götzl Rebekka , Sterzinger Sebastian , Arkudas Andreas , Boos Anja M. , Semrau Sabine , Vassos Nikolaos , Grützmann Robert , Agaimy Abbas , Hohenberger Werner , Horch Raymund E. , Beier Justus P.
Published: November 2020

Background: Soft tissue sarcoma (STS) treatment is an interdisciplinary challenge. Along with radio(chemo)therapy, surgery plays the central role in STS treatment. Little is known about the impact of reconstructive surgery on STS, particularly whether reconstructive surgery enhances STS resection success with the usage of flaps. Here, we analyzed the 10-year experience at a university hospital’s Comprehensive Cancer Center, focusing on the role of reconstructive surgery. Methods: We performed a retrospective analysis of STS-patients over 10 years. We investigated patient demographics, diagnosis, surgical management, tissue/function reconstruction, complication rates, resection status, local recurrence and survival. Results: Analysis of 290 patients showed an association between clear surgical margin (R0) resections and higher-grade sarcoma in patients with free flaps. Major complications were lower with primary wound closure than with flaps. Comparison of reconstruction techniques showed no significant differences in complication rates. Wound healing was impaired in STS recurrence. The local recurrence risk was over two times higher with primary wound closure than with flaps. Conclusion: Defect reconstructions in STS are reliable and safe. Plastic surgeons should have a permanent place in interdisciplinary surgical STS treatment, with the full armamentarium of reconstruction methods.
Perioperative Chemotherapy for Liver Metastasis of Colorectal Cancer
Authors: Chan Gloria , Chee Cheng E.
Published: November 2020

The liver is the dominant site of metastasis for patients with colorectal cancer. For those with isolated liver metastases, surgical resection with systemic therapy has led to long-term remission in as high as 80% of patients in well-selected cohorts. This review will focus on how systemic therapy should be integrated with resection of liver metastases; in particular, the use of clinical risk scores based on clinicopathological features that help with patient selection, various approaches to the treatment of micro-metastatic disease (peri-operative versus post-operative chemotherapy), as well as conversion chemotherapy for those with initially upfront unresectable disease will be discussed.
Inhibition of Serum Response Factor Improves Response to Enzalutamide in Prostate Cancer
Authors: Watson R. William , Azam Haleema , Aura Claudia , Russell Niamh , McCormack Janet , Corey Eva , Morrissey Colm , Crown John , Gallagher William M , Prencipe Maria
Published: November 2020

Castrate-resistant prostate cancer (CRPC) is challenging to treat with the androgen receptor (AR), the main target and key focus of resistance. Understanding the mechanisms of AR interaction with co-regulators will identify new therapeutic targets to overcome AR resistance mechanisms. We previously identified the serum response factor (SRF) as a lead target in an in vitro model of CRPC and showed that SRF expression in tissues of CRPC patients was associated with shorter survival. Here, we tested SRF inhibition in vitro and in vivo to assess SRF as a potential target in CRPC. Inhibition of SRF with the small-molecule inhibitor CCG1423 resulted in enhanced response to enzalutamide in vitro and reduced tumour volume of LuCaP 35CR, a CRPC patient-derived xenograft model. Nuclear localisation of AR post-CCG1423 was significantly decreased and was associated with decreased α-tubulin acetylation in vitro and decreased prostate specific antigen (PSA) levels in vivo. SRF immunoreactivity was tested in metastatic tissues from CRPC patients to investigate its role in enzalutamide response. Kaplan–Meier curves showed that high SRF expression was associated with shorter response to enzalutamide. Our study supports the use of SRF inhibitors to improve response to enzalutamide.
Women’s Intentions to Engage in Risk-Reducing Behaviours after Receiving Personal Ovarian Cancer Risk Information: An Experimental Survey Study
Authors: Gallagher Ailish , Waller Jo , Manchanda Ranjit , Jacobs Ian , Sanderson Saskia
Published: November 2020

Risk stratification using genetic and/or other types of information could identify women at increased ovarian cancer risk. The aim of this study was to examine women’s potential reactions to ovarian cancer risk stratification. A total of 1017 women aged 45–75 years took part in an online experimental survey. Women were randomly assigned to one of three experimental conditions describing hypothetical personal results from ovarian cancer risk stratification, and asked to imagine they had received one of three results: (a) 5% lifetime risk due to single nucleotide polymorphisms (SNPs) and lifestyle factors; (b) 10% lifetime risk due to SNPs and lifestyle factors; (c) 10% lifetime risk due to a single rare mutation in a gene. Results: 83% of women indicated interest in having ovarian cancer risk assessment. After receiving their hypothetical risk estimates, 29% of women stated they would have risk-reducing surgery. Choosing risk-reducing surgery over other behavioural responses was associated with having higher surgery self-efficacy and perceived response-efficacy, but not with perceptions of disease threat, i.e., perceived risk or severity, or with experimental condition. A substantial proportion of women age 45–75 years may be open to the idea of surgery to reduce risk of ovarian cancer, even if their absolute lifetime risk is only increased to as little as 5 or 10%.
Multimodal Treatment in Metastatic Colorectal Cancer (mCRC) Improves Outcomes—The University College London Hospital (UCLH) Experience
Authors: Joharatnam-Hogan Nalinie , Wilson William , Shiu Kai Keen , Fusai Giuseppe Kito , Davidson Brian , Hochhauser Daniel , Bridgewater John , Khan Khurum
Published: November 2020

Background: Despite notable advances in the management of metastatic colorectal cancer (mCRC) over the last two decades, treatment intent in the vast majority of patients remains palliative due to technically unresectable disease, extensive disease, or co-morbidities precluding major surgery. Up to 30% of individuals with mCRC are considered potentially suitable for primary or metastasis-directed multimodal therapy, including surgical resection, ablative techniques, or stereotactic radiotherapy (RT), with the aim of improving survival outcomes. We reviewed the potential benefits of multimodal therapy on the survival of patients with mCRC treated at the UCLH. Methods: Clinical data on baseline characteristics, multimodal treatments, and survival outcomes were retrospectively collected from all patients with mCRC receiving systemic chemotherapy between January 2013 and April 2017. Primary outcome was the impact of multimodal therapy on overall survival, compared to systemic therapy alone, and the effect of different types of multimodal therapy on survival outcome, and was assessed using the Kaplan–Meier approach. All analyses were adjusted for age, gender, and side of primary tumour. Results: One-hundred and twenty-five patients with mCRC were treated during the study period (median age: 62 years (range 19–89). The liver was the most frequent metastatic site (78%; 97/125). A total of 52% (65/125) had ≥2 lines of systemic chemotherapy. Of the 125 patients having systemic chemotherapy, 74 (59%) underwent multimodal treatment to the primary tumour or metastasis. Median overall survival (OS) was 25.7 months [95% Confidence Interval (CI) 21.5–29.0], and 3-year survival, 26%. Univariate analysis demonstrated that patients who had additional procedures (surgery/ablation/RT) were significantly less likely to die (Hazard Ratio (HR) 0.18, 95% CI 0.12–0.29, p < 0.0001) compared to those receiving systemic chemotherapy alone. Increasing number of multimodal procedures was associated with an incremental increase in survival—with median OS 28.4 m, 35.7 m, and 64.8 m, respectively, for 1, 2, or ≥3 procedures (log-rank p < 0.0001). After exclusion of those who received systemic chemotherapy only (n = 51), metastatic resections were associated with improved survival (adjusted HR 0.36, 95% CI 0.20–0.63, p < 0.0001), confirmed in multivariate analysis. Multiple single-organ procedures did not improve survival. Conclusion: Multimodal therapy for metastatic bowel cancer is associated with significant survival benefit. Resection/radical RT of the primary and resection of metastatic disease should be considered to improve survival outcomes following multidisciplinary team (MDT) discussion and individual assessment of fitness.
Systematic Review of Stem-Cell-Based Therapy of Burn Wounds: Lessons Learned from Animal and Clinical Studies
Authors: Henriksen Josefine Lin , Sørensen Nana Brandborg , Fink Trine , Zachar Vladimir , Porsborg Simone Riis
Published: November 2020

Treatment of severe burn wounds presents a daunting medical challenge, and novel approaches promoting healing and reducing scarring are highly desirable. The application of mesenchymal stem/stromal cells (MSCs) has been suggested as a novel treatment. In this paper, we present systematic reviews of pre-clinical and clinical studies of MSC therapy for second- or third-degree thermal burn wounds. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, the PubMed and Embase databases were searched, and interventional studies of MSC therapy using rodent models (21 studies) or human burn patients (three studies) were included in the pre-clinical and clinical reviews, respectively, where both overall outcome and wound-healing-phase-specific methodologies and effects were assessed. The pre-clinical studies demonstrated a promising effect of the application of MSCs on several wound healing phases. The clinical studies also suggested that the MSC treatment was beneficial, particularly in the remodeling phase. However, the limited number of studies, their lack of homogeneity in study design, relatively high risk of bias, lack of reporting on mode of action (MOA), and discontinuity of evidence restrict the strength of these findings. This comprehensive review presents an overview of available methodologies to assess the MOA of MSC treatment for distinct wound healing phases. Furthermore, it includes a set of recommendations for the design of high-quality clinical studies that can determine the efficacy of MSCs as a therapy for burn wounds.
Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms
Authors: Blanco-Prieto Olga , Catalán Jaime , Trujillo-Rojas Lina , Peña Alejandro , Rivera del Álamo Maria Montserrat , Llavanera Marc , Bonet Sergi , Fernández-Novell Josep Maria , Yeste Marc , Rodríguez-Gil Joan E.
Published: November 2020

This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner.
Spheroid Fabrication Using Concave Microwells Enhances the Differentiation Efficacy and Function of Insulin-Producing Cells via Cytoskeletal Changes
Authors: Lee Yu Na , Yi Hye Jin , Goh Hanse , Park Ji Yoon , Ferber Sarah , Shim In Kyong , Kim Song Cheol
Published: November 2020

Pancreatic islet transplantation is the fundamental treatment for insulin-dependent diabetes; however, donor shortage is a major hurdle in its use as a standard treatment. Accordingly, differentiated insulin-producing cells (DIPCs) are being developed as a new islet source. Differentiation efficiency could be enhanced if the spheroid structure of the natural islets could be recapitulated. Here, we fabricated DIPC spheroids using concave microwells, which enabled large-scale production of spheroids of the desired size. We prepared DIPCs from human liver cells by trans-differentiation using transcription factor gene transduction. Islet-related gene expression and insulin secretion levels were higher in spheroids compared to those in single-cell DIPCs, whereas actin–myosin interactions significantly decreased. We verified actin–myosin-dependent insulin expression in single-cell DIPCs by using actin–myosin interaction inhibitors. Upon transplanting cells into the kidney capsule of diabetic mouse, blood glucose levels decreased to 200 mg/dL in spheroid-transplanted mice but not in single cell-transplanted mice. Spheroid-transplanted mice showed high engraftment efficiency in in vivo fluorescence imaging. These results demonstrated that spheroids fabricated using concave microwells enhanced the engraftment and functions of DIPCs via actin–myosin-mediated cytoskeletal changes. Our strategy potentially extends the clinical application of DIPCs for improved differentiation, glycemic control, and transplantation efficiency of islets.
Genetic Consequences of Multiple Translocations of the Banded Hare-Wallaby in Western Australia
Authors: White Daniel J. , Ottewell Kym , Spencer Peter B. S. , Smith Michael , Short Jeff , Sims Colleen , Mitchell Nicola J.
Published: November 2020

Many Australian mammal species now only occur on islands and fenced mainland havens free from invasive predators. The range of one species, the banded hare-wallaby (Lagostrophus fasciatus), had contracted to two offshore islands in Western Australia. To improve survival, four conservation translocations have been attempted with mixed success, and all occurred in the absence of genetic information. Here, we genotyped seven polymorphic microsatellite markers in two source (Bernier Island and Dorre Island), two historic captive, and two translocated L. fasciatus populations to determine the impact of multiple translocations on genetic diversity. Subsequently, we used population viability analysis (PVA) and gene retention modelling to determine scenarios that will maximise demographic resilience and genetic richness of two new populations that are currently being established. One translocated population (Wadderin) has undergone a genetic bottleneck and lost 8.1% of its source population’s allelic diversity, while the other (Faure Island) may be inbred. We show that founder number is a key parameter when establishing new L. fasciatus populations and 100 founders should lead to high survival probabilities. Our modelling predicts that during periodic droughts, the recovery of source populations will be slower post-harvest, while 75% more animals—about 60 individuals—are required to retain adequate allelic diversity in the translocated population. Our approach demonstrates how genetic data coupled with simulations of stochastic environmental events can address central questions in translocation programmes.
Characterization of Pathogen Airborne Inoculum Density by Information Theoretic Analysis of Spore Trap Time Series Data
Authors: Choudhury Robin A. , McRoberts Neil
Published: November 2020

In a previous study, air sampling using vortex air samplers combined with species-specific amplification of pathogen DNA was carried out over two years in four or five locations in the Salinas Valley of California. The resulting time series data for the abundance of pathogen DNA trapped per day displayed complex dynamics with features of both deterministic (chaotic) and stochastic uncertainty. Methods of nonlinear time series analysis developed for the reconstruction of low dimensional attractors provided new insights into the complexity of pathogen abundance data. In particular, the analyses suggested that the length of time series data that it is practical or cost-effective to collect may limit the ability to definitively classify the uncertainty in the data. Over the two years of the study, five location/year combinations were classified as having stochastic linear dynamics and four were not. Calculation of entropy values for either the number of pathogen DNA copies or for a binary string indicating whether the pathogen abundance data were increasing revealed (1) some robust differences in the dynamics between seasons that were not obvious in the time series data themselves and (2) that the series were almost all at their theoretical maximum entropy value when considered from the simple perspective of whether instantaneous change along the sequence was positive.
Digital Signal, Image and Video Processing for Emerging Multimedia Technology
Authors: Kim Byung-Gyu
Published: November 2020

Recent developments in image/video-based deep learning technology have enabled new services in the field of multimedia and recognition technology [...]
Short Review: Timeline of the Electrochemical Lithium Recovery System Using the Spinel LiMn2O4 as a Positive Electrode
Authors: Joo Hwajoo , Lee Jaehan , Yoon Jeyong
Published: November 2020

Various lithium recovery technologies have been developed as securing lithium resources has become increasingly important. Among these technologies, the electrochemical lithium recovery (ELR) system is a rapid and eco-friendly extraction method that has been studied recently. In this paper, an ELR system using a spinel-type LiMn2O4 (LMO) is briefly reviewed. As LMO electrodes have high Li+ selectivity and stability compared to other lithium battery cathodes, they have been widely used as positive electrodes for the ELR system. This paper summarizes the system proposal, LMO electrode modification, system analysis, and industrial applications. Perspectives of the ELR technology are presented considering the progress of the research.
Deep Learning Based Successive Interference Cancellation Scheme in Nonorthogonal Multiple Access Downlink Network
Authors: Sim Isaac , Sun Young Ghyu , Lee Donggu , Kim Soo Hyun , Lee Jiyoung , Kim Jae-Hyun , Shin Yoan , Kim Jin Young
Published: November 2020

In this paper, a deep learning-based successive interference cancellation (SIC) scheme for use in nonorthogonal multiple access (NOMA) communication systems is investigated. NOMA has become a notable technique in the field of mobile wireless communication because of its capacity to overcome orthogonality, unlike a conventional orthogonal frequency division multiple access (OFDMA) communication system. In NOMA communication systems, SIC is one of the decoding schemes applied at receivers for downlink NOMA transmissions. In this paper, a convolutional neural network (CNN)-based SIC scheme is proposed to improve performance of the single base station and multiuser NOMA scheme. In contrast to existing SIC schemes, the proposed CNN-based SIC scheme can effectively mitigate losses resulting from imperfections of the SIC. The simulation results indicate that the CNN-based SIC method can successfully relieve conventional SIC impairments and achieve good detection performance. Consequently, a CNN-based SIC scheme can be considered as a potential technique for use in NOMA detection schemes.
Utilization of Gaseous Carbon Dioxide and Industrial Ca-Rich Waste for Calcium Carbonate Precipitation: A Review
Authors: Czaplicka Natalia , Konopacka-Łyskawa Donata
Published: November 2020

Technologies for the management of various types of waste and the production of useful products from them are currently widely studied. Both carbon dioxide and calcium-rich waste from various production processes are problematic wastes that can be used to produce calcium carbonate. Therefore, the purpose of this paper is to provide an overview about the state of the development of processes that use these two wastes to obtain a valuable CaCO3 powder. The paper reviews the current research on the use of post-distillation liquid from the Solvay process, steelmaking slag, concrete, cement, and gypsum waste as well as some others industrial Ca-rich waste streams in the calcium carbonate precipitation process via carbonation route. This work is an attempt to collect the available information on the possibility of influencing the characteristics of the obtained calcium carbonate. It also indicates the possible limitations and implementation problems of the proposed technologies.
Are BBQs Significantly Polluting Air in Poland? A Simple Comparison of Barbecues vs. Domestic Stoves and Boilers Emissions
Authors: Badyda Artur , Krawczyk Piotr , Bihałowicz Jan Stefan , Bralewska Karolina , Rogula-Kozłowska Wioletta , Majewski Grzegorz , Oberbek Przemysław , Marciniak Andrzej , Rogulski Mariusz
Published: November 2020

The study attempts to compare the total annual emissions of selected air pollutants emitted during occasional grilling and the emission of the same pollutants from small domestic heating installations. For this purpose, in the absence of any data on the emission of pollutants during grilling processes, tests were carried out consisting of measuring the concentration of air pollutants in exhaust streams from two types of grills (solid fuel grill powered by charcoal briquette and gas grill powered by liquid propane), using popularly prepared dishes (previously marinated meat and raw, seasoned mixed vegetables). The concentrations of PM2.5, CH4, CO, CO2, H2O, NH3, N2O, NO, NO2, SO2 were measured in the exhaust stream from both grills using a particulate matter (PM) measuring device and a portable spectrometer, separately while grilling the same portions of meat and vegetables. Then, considering the available data on Poles’ barbecue habits, the emissions that are released into the air during occasional grilling were estimated. The calculated emissions were compared with the data on emissions from domestic heating installations used in Poland. It has been shown that during grilling, as much as 2.30, 92.07, 4.11, 3.83, 2.96, and 9.81 Gg of PM2.5, CO, NOx, SO2, NH3, and CH4 may be released into the atmosphere in Poland, respectively. In the case of PM, the amount of the pollutant emitted to the air is over 100 times lower than the emissions caused by the operation of small heating installations. In the case of other pollutants, the differences are smaller. Nevertheless, emissions from grills should not be underestimated as, in certain periods of the year, these sources may be responsible for not meeting the air quality standards in selected areas of the country, and thus the excessive exposure of people to pollutants resulting in negative health consequences. Therefore, attention was paid to the legitimacy of abandoning the use of charcoal and charcoal briquette grills and replacing them with gas-powered grills or electric ones, not only due to the health benefits of food and lower human exposure, but also by the reason of ecological values.
Effects of a Crossarm Brace Application on a 275 kV Fiberglass-Reinforced Polymer Crossarm Subjected to a Lightning Impulse
Authors: Abd Rahman Muhammad Syahmi , Ab Kadir Mohd Zainal Abidin , Ab-Rahman Muhamad Safwan , Osman Miszaina , Mohd Nor Shamsul Fahmi , Mohd Zainuddin Noorlina
Published: November 2020

The crossarm is an important component of transmission towers, providing insulation for transmission lines at different voltage ratings. Recently, composite crossarms were widely used as a composite tower component and were found to be the most favorable choice for replacing old wooden crossarms. Owing to the satisfactory pilot operation and multiple sets of testing, fiberglass-reinforced polymer (FRP) composite crossarms have been used in Malaysia in both 132 and 275 kV transmission lines since the late 1990′s. Since then, some modifications have been proposed to improve the mechanical performance of the crossarm, in order to ensure the reliability of its performance. In this investigation, the effect of a proposed improvement, achieved by installing a brace for the crossarm, was investigated numerically. A simulation study was conducted, with a consideration of the lightning impulse voltage (LIV) and swing angle exhibited by the crossarm. The potential and electric field (E-Field) distribution were analyzed and are presented in this paper. It was found that the potential distribution and E-Field strength for the crossarm and the surrounding air were greatly affected by the installation of the brace.
Emission and Neutralization of Methane from a Municipal Landfill-Parametric Analysis
Authors: Ciuła Józef , Kozik Violetta , Generowicz Agnieszka , Gaska Krzysztof , Bak Andrzej , Paździor Marlena , Barbusiński Krzysztof
Published: November 2020

An attempt was made to estimate the annual production of CH4 at a municipal waste landfill site in Poland. As a matter of fact, the extent of the unorganized emission of CH4 from the landfill surface was approached based on the adopted mathematical model. The Ward agglomeration method for cluster analysis and the Pearson coefficient were employed to evaluate the distance-based similarity measure and to optimize methods for estimating methane emissions from a landfill as well as to verify the input parameters for the model. In order to calculate the content of biodegradable organic parts in the waste, morphological tests of the landfilled waste were performed. Physical quantities, measurements and the actual amount of the landfilled waste as well as the volume of CH4 neutralized in a collective flare were implemented in the model, respectively. The model-based findings and experimental outcome demonstrated stable gas production in the landfill with a high CH4 content. On the other hand, a rather low efficiency of the landfill passive degassing installation indicated the necessity to design and develop its active counterpart with the prospective application of the generated biogas for energy production in a cogeneration system.
Exploring the Relationship between Forest Structure and Health
Authors: Kim Jinki , Park Duk-Byeong , Seo Jung Il
Published: November 2020

There is abundant evidence that green space in urban neighborhood is associated with physical activity and it is well known that physical activity contributes to human health. Physical activity fosters normal growth and development, can reduce the risk of chronic diseases, and can make people feel better and function better. Evidences also show that exposure to natural places can lead to positive mental health outcomes, whether a view of nature from a window, being within natural places, or exercising in these environments. The study aims to identify the factors of forest structure and socioeconomic characteristics influencing adults’ physical activity and health. A sample of 148,754 respondents from the Korea Community Health Survey, conducted in 2016, was analyzed. Measures included frequency of physical activity, stress, depression, and landscape metrics of forest patch. Hierarchical multiple regression analysis, controlling for socio-demographic characteristics, revealed that larger forest patches and the more irregular shapes were associated with more physical activity. The study also showed that the shape of forest patch and slope were associated with less mental health complaints, whereas composition related landscape metrics were not.
Comprehensive Accounting for REDD+ Programs: A Pragmatic Approach as Exemplified in Guyana
Authors: Goslee Katherine M. , Pearson Timothy R. H. , Bernal Blanca , Simon Sophia L. , Sukhdeo Hansrajie
Published: November 2020

Completeness is an important element for Reducing Emissions from Deforestation and forest Degradation (REDD+) accounting to ensure transparency and accountability. However, including a full accounting for all emission sources in a REDD+ program is often resource-intensive and cost-prohibitive, especially considering that some emission sources comprise far less than 10% of total emissions and are thus considered insignificant according to Intergovernmental Panel on Climate Change (IPCC) guidance. This is evident in forest reference emission level (FREL)/forest reference level (FRL) submissions to the United Nations Framework Convention on Climate Change (UNFCCC). Of the 50 countries that have submitted FRELs to date, only half of them include degradation in their FRELs even though degradation is often a significant source of emissions. Half of the countries that do include degradation use satellite imagery without necessarily specifying degrading activities or separating anthropogenic activities. Guyana provides an example of an approach that enables inclusion of all emission sources while considering the significance of each when developing an accounting approach. Since submitting its FREL in 2014, Guyana has made stepwise improvements to its emission estimates so that the country is now able to report on all deforestation and degradation activities resulting in emissions, whether significant or not. Based on the example of Guyana’s efforts, the authors recommend a simple approach to move towards complete accounting in a cost-effective manner. This approach can be scaled to other countries with other activities that results in greenhouse gas emissions from deforestation and forest degradation. Such complete accounting allows for higher accountability in REDD+ systems and can lead to greater effectiveness in reducing emissions.
Effect of Low-Temperature-High-Pressure Treatment on the Reduction of Escherichia coli in Milk
Authors: Li Yifan , Zheng Zhuoyun , Zhu Songming , Ramaswamy Hosahalli S. , Yu Yong
Published: November 2020

Non-thermal processing of milk can potentially reduce nutrient loss, and a low-temperature-high-pressure (LTHP) treatment is considered as a promising alternative to thermal treatment, attracting considerable attention in recent years. The effect of LTHP treatment (−25 °C, 100–400 MPa) on the phase transition behavior of frozen milk was evaluated. The lethal and injured effects of different pressures and cycle numbers on E. coli in frozen milk were studied by using selective and non-selective enumeration media. Results from the gathered transient time–temperature–pressure data showed that pressures over 300 MPa could induce a phase transition from Ice I to Ice III. The treatment at −25 °C and 300 MPa could achieve a lethal effect similar to the two-cycle treatment of 400 MPa at room temperature. This meant that LTHP conditions can lower the operating pressure by at least 100 MPa or reduce the operation from two cycle to one cycle. Increasing the number of pressure cycles enhanced the lethal effects, which was not additive, but resulted in a transformation of part of the injured cells into dead cells. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) provided direct evidence for the breakdown of cell membrane and cell walls by phase transitions. Combined with a designed internal cooling device, the LTHP process can be expected to be a more attractive alternative to non-thermal processing for the dairy industry.
Preparation of Protein Oleogels: Effect on Structure and Functionality
Authors: Feichtinger Annika , Scholten Elke
Published: November 2020

Among available structuring agents that have been used to provide solid properties to liquid oils, protein is a more recent candidate. Due to their nutritional value and high consumer acceptance, proteins are of special interest for the preparation of edible oleogels as an alternative for solid fats. Whereas the field of protein oleogelation is still rather new and just starts unfolding, several preparation methods have been demonstrated to be suitable for protein oleogel preparation. However, there is limited knowledge regarding the link between microstructural properties of the gels and macroscopic rheological properties, and the potential of such protein-based oleogels as a fat replacer in food products. In this review, we therefore provide an overview of various protein oleogel preparation methods and the resulting gel microstructures. Based on the different structures, we discuss how the rheological properties can be modified for the different types of protein oleogels. Finally, we consider the suitability of the different preparation methods regarding potential applications on industrial scale, and provide a short summary of the current state of knowledge regarding the behavior of protein oleogels as a fat replacer in food products.