bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Strategies for Fabrication of Die Assembly with Typical Shaped Cavity by Using Electrical Discharge Machining
Authors: Feng Ren Yu , Wang Min , Duan Zhi Jian
Published: July 2012

Die assembly is a kind of special tools for fabrication of metal or non-metallic materials parts with required design and purpose. Generally, the high material hardness for die assembly should be selected to increase the service life and reduce the cost in manufactory industry. Electrical discharge machining (EDM) is a suitable method for fabrication of die assembly as the material remove in EDM process mainly depends on the material's thermal characteristics, regardless of the hardness. In this paper, the machining strategies for fabrication of die assembly with typical shaped cavity using EDM were discussed, according to the different cavity structures, the best machining strategies were proposed
Research on Hydraulic System of Speed Forging Hydraulic Machine
Authors: Yu Hao Yang , Tang Jing Lin , Hu Jing
Published: July 2012

In recent years, industrial technology in China continues developing fast, and this has put forward higher requirements for China's forging equipment. The development international direction of the forging equipment is multi-bit, high-speed and large-set, which leads to the combination of a number of technologies such as hydraulic, pneumatic, electronic, mechanization and detection. However, China's forging press is still at a relatively low level. The organically combination of CNC system with the computer, network, automated warehouses, automatic mold change systems and industrial robots has made the development of the manufacture more and more intelligent as well as its technology level more and more intelligent. In order to occupy a place in the international arena, it is imminent to vigorously develop China's forging equipment. As high speed forging hydraulic machine occupies a very important position in the whole forging system, and the requirement on the dimensional accuracy and productivity of industrial products is getting higher and higher in modern society. This paper introduces the analysis and researches on the hydraulic system of high speed forging hydraulic machine, which is also the difficult and hot point of social researches.
Using Force/Torque Balance Equations and CAD Variable Geometric Approach for Solving Active Force and Passive Force of a 3UPU-I Spatial Parallel Manipulator
Authors: Ye Yong , Liu Nan
Published: July 2012

A CAD variation geometry approach and force/torque balance equations are proposed for solving active force and passive force of a 3-dof 3UPU-Ⅰspatial parallel manipulator. Some common math foundations, computer variation geometry techniques, and definitions are presented for designing F/T (force/torque) simulation mechanism based on simulation mechanism of the parallel manipulator. A force Jacobian matrix equation Gf can be derived from the balance equations of the manipulator, when modify the driving dimensions of active limbs, the configuration of the F/T simulation mechanisms are varied correspondingly, and a Matlab program is compiled for solving the active force and passive force the active forces.
Study on Statistic and Analysis of Types of Slots Centering Relation
Authors: Liang She Hui
Published: July 2012

The types of slots selected in the system of relation of slots centering is studied. The analysis leads to such conclusions as important position of quantity attribute, concrete state, possessive generally in front of slots link and the article also classifies the reasons.
Design of Optical Perfection of Flame Detector
Authors: Wang Yong Yu , Mei Zhi Bin , Xu Fang , Zhang Xi
Published: July 2012

This paper summarizes the current situation home and abroad of the detection technology of point flame detector. It briefly introduces the application situation of flame detector engineering and describes the problems exist in actual project application of the point flame detector. Based on the theoretical optics of flame detector, combining the optics testing platform, collection work on combustion flame of characteristic materials and characteristic source spectrum has been carried out. Through carrying out relevant experiments, relevant data has been collected and analyzed. Select automatic optical inspection focusing on flame detectors focusing on different wave bands. The optical perfection design scheme is put forward in the end based on point flame detector of reflecting light source.
Static and Dynamic Performance of High-Speed Vertical Machining Center Spindle Box
Authors: Li Rong Chang , He Ai Xia
Published: July 2012

In order to improve the static performance of high speed machining center spindle box, using ANSYS software static performance analysis, static analysis of finite element method to the complex structure of the spindle box, draw a box structure under the force load and temperature load stiffness theoretical values, emphasizing the factors affecting temperature deformation, provides a basis to improve and control the strength and stiffness of the spindle box, as well as box optimization design of the design constraints.
A Thermoelectric Modules Voltage Monitoring System Used in Automobile Exhaust Thermoelectric Generator
Authors: Quan Rui , Tang Xin Feng , Quan Shu Hai , Huang Liang
Published: July 2012

According to the requirement of independent voltage monitoring of thermoelectric modules used in Automobile Exhaust Thermoelectric Generator(AETEG) designed by our group, a kind of voltage monitoring system based on master-slave architecture that includes several slave detecting boards and a master detecting board was designed in this paper, the hardware schematic and software flow diagram were provided, the amplifier circuit that could detect negative voltage of thermoelectric modules was designed, and the PC based monitoring interface using VC++ language was developed as well. The practical application results validate that the system designed is sophisticated and steady, it meets the real time voltage monitoring demand of thermoelectric modules and provides convenience for their optimization of electric topology structure in future.
The Design, Numerical Simulation and Experimental Research on Low Resistance Diffluence T-Junction
Authors: Tao Hai Kun , Zhou Yong , Wei Zhi Guo , Zhang Hao , Li Yong
Published: July 2012

A new type of diffluence T-junction with low-resistance was designed to reduce the flow resistance coefficient. After the numerical simulation on internal flow field for both the traditional and newly-invent T-junction, an analysis was made on their flow pattern, and their fluid resistance coefficient was computed. At last, the actual fluid resistance was measured respectively. It shows that: the results from numerical simulation agree well to those got in experimental testing. Furthermore, the flow resistance coefficient of T-junction is decreased from 1.13 to 0.61 after redesign, which indicates high effectiveness of this process. The mechanism is that smoothness for branch can inhibit the vortex generation efficiently and greatly reduce its flow resistance coefficient, which consequently reduces the gross flow resistance
Research for Straightening of TC4 Alloy Wire Rod Based on Horizontal-Vertical Roller System
Authors: Zhang Chao , Feng Jian Chao , Chen Ming He
Published: July 2012

Straight TC4 wire rod is demanded by many industries, however the technology of straightening for it has not been solved. In the present paper, a horizontal-vertical roller thermal straightening system is designed and the theory of rolling reduction is concluded. Based on the new system and the rolling reductions calculated for each roller, the method of finite element analysis is used to show the process of straightening at 600°C. The result of straightening is ideal, which proves the straightening system is adequate for Φ4.5 TC4 wire rod.
Purification and Characterization of an Alkaline Protease from Bacillus alcalophilus
Authors: Liu Shan Shan , Wang Li Li , Yuan Lin
Published: July 2012

The purification and characterization of an alkaline protease produced by Bacillus alcalophilus were investigated. The enzyme was purified in two steps: concentrating the crude enzyme using ammonium sulfate precipitation, followed by cation-exchange chromatography. The purified protease had a molecular mass of approximately 28 kDa, was highly active over an alkaline pH range of 10.0 to 11.0, and remained stable over a pH range of 7.0 to 12.0. The optimum temperature for the enzyme activity was found to be 40~60°C, while the thermotolerance of the enzyme was poor. Therefore, these characteristics of the protease indicate its potential for a wide range of commercial applications.
The Prospect of Nano Vegetable Transformer Oil
Authors: Xu Peng Fei
Published: July 2012

This paper started with the performance of ordinary transformer oil, and then compared the Nano-modified vegetable oil with transformer oil. From the comparison of results, the author drew a conclusion that Nano vegetable oil must have broad prospects in the use of the transformer.
The Study on Isolated Power Control System Based on Coordinated Control
Authors: Xu Yong Sheng
Published: July 2012

This paper analysis the formation conditions of isolated power system, its influencing factors, revealing the operation mode, automatic safety device, generator protection regulation characteristics and these factors to stable operation of isolated power system. The dynamic models for isolated power system with generating unit of medium and small capacity are proposed in the paper to meet the need of the applicable ones to study their dynamic characteristics. The differences between isolated and the interconnected power system in the frequency characteristics are proposed and consider into low-frequency load shedding scheme and optimization methods that can meet both isolated and the interconnected power system frequency stability requirements. Not only more proper control strategy of units but their evaluation indexes are suggested to improve performance and achieve stability in islanded power system.
Study on Design and Implementation of Integrated Access System of Intelligent Transportation
Authors: Long Qiong , Huang Zhi Fang , Zhang Jin Fan
Published: July 2012

Intelligent transportation integrated access system can significantly improve the security of computer network systems, while trusted computing technology can effectively enhance the credibility and safety of the terminal, and then construct a trusted network connection. Based on computing technology, this thesis proposes to establish a credible integrated access network, and implement network access control on the remote access equipment, thereby enhancing the safety of the integrated access system. The results show that, through the transmission technology of credible chain proposed in this paper, and remote proof technology basing on device identity and security policies can establish a credible integrated access system, thus effectively improving the security of Beijing intelligent transportation systems.
Research on Parameter Tuning Method for Numerical Control AC Servo Control System
Authors: Zheng Hong
Published: July 2012

Equipment manufacturing industry is an important factor to measure a country's comprehensive national strength. The servo control technology is one of the core technologies of numerical control systems and motion control, as well as an important part of the equipment manufacturing. This paper, on the basis of motion control, studies the development status of servo control home and abroad and analyzes in-depth the various components of the AC servo control system and the parameter confirmation of the controller. It discusses the approximate processing on control system and simplification of passing functions and uses the best second-order tuning to set the project for the current loop Pl adjustment device, simplifying the current loop into an inertia link.
Research on Intelligent Fault Diagnosis System Based on Numerical Turning Machine
Authors: Zhang Tao , Wang Qiu Hong , Han Jiang
Published: July 2012

According to the problem of NC Turning Machine fault diagnosis,this paper use frame as express of fault case,through XML case of storage and retrieval,which can realize the intelligent fault diagnosis reasoning Based on CBR. The redevelopment data stored of the fault cases in the XML neutral document,while XML doucument has the advantage of small data quantity and strong universal make it exchange and transmission easily. provided a new way of data exchange and information integration.
Improved Localization Algorithm Based on Proportion of Differential RSSI
Authors: Zhang Kai Sheng , Xu Ya Ming , Yang Wu , Zhou Qian
Published: July 2012

How to enhance the accuracy of sensor node self-localization for limited energy resource networks is an important problem in the study of wireless sensor networks (WSNs). Concerning the advantages and disadvantages of some main algorithms for senor node self-localization, an easy and simple algorithm is proposed to locate the unknown node itself. The algorithm is to improve weight centroid localization (WCL), by the way of determining weight through using the proportion of differential received signal strength indicator (RSSI) that are derived from unknown node and criterion nodes. In contrast to WCL, the algorithm has the strengths of less computation and better determination of weight, and the determination of weight shows more distinguished distinction in the effect on the localization of unknown node, which is caused by various beacon nodes. Simulations demonstrate that the algorithm has a higher localization accuracy than WCL
The Application of Linear Algebra Algorithm in the Production of Linear Matrix Inequalities
Authors: Zhang Hui
Published: July 2012

Discusses the theory and symbolic of the algorithm gives another potential application, but also in the system and control. For example, for the question, has made with special structure, but LMI problem data, may cause factorizations LMI more compact. One can even imagine using the algorithm around, looking for the opportunity to LMI automatic eliminate variables, so simplify problem solving, before they get a lot of influence and a potential solutions. We describe theory, the algorithm can be used to factor in the non commuting variable polynomial matrix and application system switches and control problem into a linear matrix inequality.
Parameter Identification of Tailplane Iced Aircraft Based on Maximum Likelihood Method
Authors: Xu Zhong Da , Cao Yi Hua , Zhao Ming
Published: July 2012

Based on the maximum likelihood method, this paper analyzed the influence of tailplane icing on aerodynamic parameters by parameter identification. A nonlinear longitudinal flight dynamics model for aircraft was built, and an identification system was constructed using maximum likelihood method. According to the flight test data of DHC-6 aircraft, the aerodynamic parameters of clean aircraft and two different cases of iced aircraft with particular ice shapes on the tailplane were identified. Eventually, the results of the identification show that the tailplane icing has several adverse effects on the aircraft flight characteristics, including flight performance, elevator effectiveness, stability and safety.
Study on Simulation Analysis of XXX Machine Cutting Chatter
Authors: Liu Yu Chang , Liu Zhi Peng , Jin Yan , Sheng Zhong Qi , Liu Yong Xian
Published: July 2012

According to regenerative chatter theory, this paper abstracts the machining process of XXX machine as a mathematical model firstly. The main process is end milling, so this paper abstracts the machine as an end-milling mathematical model, a delay motion equation with 2-DOF. All parameters of this equation can be acquired by modal analysis. Then SIMULINK model is created based on the mathematical model. According to the response characteristics of simulation analysis system, the method of determining threshold of steady area is acquired.
Failure Analysis of Commercial Vehicle Crankshaft: A Case Study
Authors: Bai Shu Zhan , Hu Yu Ping , Zhang Hai Long , Zhou Sheng Wei , Jia Ying Jun , Li Guo Xiang
Published: July 2012

A failure analysis has been conducted on a diesel engine crankshaft used in a commercial vehicle, which is made from 42CrMo forging steel, and the crankshaft was after induction hardening process. The fracture occurred between the 4th journal and the 4th crankpin, fracture section indicates that fatigue is the dominant mechanism of failure for this crankshaft. In order to find the failure causes, the dimension of journal boss fillet, material chemical compositions, surface hardness and depth of hardness layer were evaluated. Chemical compositions of crankshaft material, surface hardness and the depth of hardness layer are within the specified range, however the dimension of journal boss fillet is less than specified, and the transition zone of hardness layer was found on the journal boss fillet. Heavy segregation and many non-metallic inclusions aggregate zones were found from metallographic, these would lead to the reduction of endurance bending strength. FEA analysis results suggest that the dimension of journal boss fillet have an important effect on the stress and stress amplitude of the crankshaft. So the disqualification machining and material property are the main causes for the crankshaft fatigue fracture.
The Study of Forward Slip Model in Round-Oval-Round Bar Rolling
Authors: Zhou Jia Lin , Pan Cheng Gang , Lv Bing , Wang Hui
Published: July 2012

In order to predict the forward slip value correctly, this article established regression model of forward slip in oval-round and round-oval pass rolling, predicted the forward slip values in ach pass in Ф12 and Ф16 round bar rolling for a factory, measured forward slip of lead rods in experimental mill using geometrical modeling method. The results showed that the model of forward slip has great practical value for proper design rolling speed, rolling schedule, thereby ensuring the continuous rolling process carried out smoothly
Research on the Correlation between Neuronal Damage and Drug Resistance Gene Product P-Gp Following Status Epilepsy
Authors: Liu Song Yan , Mao Rui Tao , Cai Hua , Han Xue Mei
Published: July 2012

Objective: To study the association between neuronal injury following status epilepsy and expression of P-glycoprotein, discuss the association between intractable epilepsy and neuronal injury. Method: To establish a mice status epilepticus model by lithium-pilocarpine injected intraperitoneal, The mice were randomly divided into control group 6h, 12h, 1d, 3d, 5d and 7d after status epilepticus(SE) .To research the neuronal injury by image analysis method, expression of P-gp in blood was studied by flow cyometry .To analyze the association between neuronal injury and expression of P-gp by Pearson statistics. Result: The neuronal injury was observed after status epilepsy. Neuronal injury is most obvious 3-7 days after status epilepsy, expression of P-gp increased simultaneously. The severity of neuronal injury has a positive correlation to expression of P-gp. Conclusion: Association between intractable epilepsy and neuronal injury exists.
Study on the Causes and Prevention Countermeasures of Algal Bloom for Wuliangsuhai Lake in Inner Mongolia
Authors: Jiang Zhong Feng , Li Chang You , Zhang Sheng
Published: July 2012

“Algal Bloom” have been broken in Wuliangsuhai lake in May 2008 and in May 2009, this situation has caused some loss of lake inherent function,for example aquaculture, irrigation, tourism and so on, at the same time,severely restricting the sustainable development of regional economy. It is a very important Question for “algal Bloom” need to be concerned of leads and scholars.Water quality, hydrology, meteorology etc Monitoring data were analyzed for the "algal Bloom" period. The results show: The main reasons of “algal Bloom” were many beneficial factors for algal growth and water eutrophication in the state of long-term.
The Isolation and Prospect of Biosurfactants
Authors: Xu Yun Yun , Zhang Tao , Li Xin Nian , Chen Lei , Wang Hao
Published: July 2012

Biosurfactants are natural surface-active compounds mainly synthesized by microorganisms, which have distinct advantages like no secondly pollution and friendly to environment compared with chemical surfactants. With the development of modern biological technology, biosurfactants have been shown a variety of potential applications, including medicine, agriculture, oil production and environmental remediation, so it has already caused many researchers a strong interest in the production of biosurfactants making use of biological technology. A review is made from the isolation of biosurfactants. In addition, on the foundation of the analysis,several suggestions about the development of biosurfactants are proposed.
Structural Analysis Based on 16S rDNA Sequences of Eight Strains of Leptospirillum ferriphilum from Acid Mine Drainage or Hot Spring
Authors: Gao Jian , Kang Jian , Qiu Guan Zhou
Published: July 2012

The correlations between the phylogenetic similarity and original geographical sites of L. ferriphilum were evaluated on the basis of 16S rDNA sequence analysis. Results indicated that all of L. ferriphilum strains in this work may be classified into three phylogenetic groups, and there were some certain links between phylogenic similarity and the mineralogical characteristics. Furthermore, structural analysis of 16S rDNA sequences suggested that nucleotide variables (signature sites) were not randomly distributed. Of 23 signature sites detected, 17 positions were found in the first 600 bp from 5' terminal except position 1020, 1022, 1023, 1147, 1155 and 1277. Interestingly, 13 positions of them were found in the V6 region. Thus, the secondary structure and minimal free energy were determined in the V6 region among strains of three phylogenetic groups. The above results may be very useful in helping to screen bioleaching microorganisms with great application prospect and discerning the microevolutionary mechanisms of species formation.
Effects of Liquid-to-Powder Ratio on the Properties of Self-Setting Silk Fibroin/Calcium Phosphate Composites
Authors: Xie Rui Juan , Huang Yang Yang , Wang Biao , Zhang Meng
Published: July 2012

In this study, the high purity tetracalcium phosphate (TTCP) was prepared conveniently after studying the effect of cooling method on the purity of TTCP. The influence of liquid-to-powder (L/P) ratio on the properties of self-setting silk fibroin/calcium phosphate composites was studied. The results showed that the temperature of the furnace had a significant influence on the purity of TTCP when samples were removed from the furnace. Whether using N2 or not had no obvious effect on the purity of TTCP when the temperature declined from 1500°C to 1300°C. The setting time of composites became longer with the increase of L/P ratio in a range 0.32 - 0.39 ml/g, but all less than 15min. The injectability of the composites improved significantly as the L/P ratio increased. The compressive strength of composites reached maximum at the L/P ratio of 0.34 ml/g. The compressive strength decreased with the increase of L/P ratio greater than 0.34ml/g. The L/P ratio had no significant effect on the structure of the composites and the rod-like crystal of hydroxyapatite appeared in all the hardening-body of composites.
Effect of Cadmium on Element in the Root of Mung Bean Seedlings
Authors: Zhang Yuan Hua
Published: July 2012

The metobolism of K, Ca, Mg and microelement in the root of Phaseolus raditus L. were studied. By flame atomic absorption spectromentry (FAAS), the concentration of K, Ca, Mg, Zn, Cu, Fe and Mn in the root of mung bean seedlings at the initial stage of growth under cadmium stress (1 μmol/L) were determined. It shows that the concentration of K, Mg and Mn in the root are decreased，but Ca, Zn, Cu, and Fe are increased. So, it indicated that Cd did harm, indirectly, to the root of mung bean seedlings at the initial stage of growth.
Combined PEG Immobilization on Polystyrene Microspheres: Ability of Protein Resistance
Authors: Xiao Xi Feng , Zhou Lei Ji
Published: July 2012

Polyethylene glycol (PEG) fixation has been proved to significantly decrease nonspecific protein adsorption on varied substrates. Here, a strategy of surface modification using two kinds of PEGs with different chain lengths was developed to improve the surface ability of protein resistance. We designed different reaction routes and investigated their reaction conditions. The amount of nonspecific protein adsorption on the substrates after modification was measured by UV-Vis spectrometry. The results showed that higher performance of protein resistance on the solid surface was acquired from the two-step modification than other methods. A competitive mechanism of reaction was proposed to elucidate the interaction between distinct PEGs on the substrates.
pH-Shift Control Strategy for Butyric Acid Production by Clostridium thermobutyricum
Authors: Liu Yin , Zhang Fei , Zhao Ji Hong , Wei Ming Bao , Yang Xue Peng
Published: July 2012

Batch fermentations by Clostridium thermobutyricum ATCC49875 at various pH values ranging from 5.9 to 6.8 were investigated. Based on the analysis of the experimental results, a two-stage pH-shift control strategy was proposed, with the aim of improving the efficiency of butyric acid production. At the first 24 h, pH was controlled at 6.8 to obtain the higher μx, and subsequently pH 6.2 was used to maintain high μp to enhance the production of butyric acid. Compared to control group (those of constant pH operations), a fermentation process by using this control strategy for butyric acid production had a significant improvement on the maximal butyric acid (24.60 g/l vs. 20.06 g/l) and glucose conversion efficiency (49.20% vs. 46.33%). Fed-batch fermentation by using pH-shift control strategy was also applied to produce butyric acid; and the maximal butyric acid yield and glucose conversion efficiency reached 28.75 g/l and 47.92%, respectively. These results showed that pH-shift fermentation could serve as an excellent alternative to conventional butyric acid fermentation.
Isolation of Rhizopus Producing Esterifying Enzyme and Study on its Enzyme Production Conditions
Authors: Huang Dan , Shang Zhi Chao , Li Yu Long , Chu Yu Long , Yuan Xian Ling
Published: July 2012

A Rhizopus strain producing esterifying enzyme was isolated from Luzhou-flavor DaQu. It was preliminary identified as Rhizopus arrhizus. Through the research of the culture conditions of the strain producing esterifying enzyme, it was found that the enzyme activity could reach 7.91U/mL as 2% corn meal used as C source, 6% peptone used as N source, initial pH value was 6.0, and 72h culture with 150 r/min agitation at 30°C.
Selective Catalytic Reduction of NOx on VCuMn/TiO2 at Middle-Low Temperature
Authors: Chen Zhi Hang , Tang Zhi Xiong , Cen Chao Ping , Zeng Huan Mu , Chen Ding Sheng , Fang Ping
Published: July 2012

In this dissertation, combinatorial chemistry was adopted, and the activities of a series of VCuMn/TiO2catalysts prepared by impregnation method for middle-low temperature SCR of NOxwere evaluated. The activity datas showed that 2V2Cu8Mn/TiO2exhibited better activity. At 200 °C, above 70% NOxconversion were both obtained with a gas hourly space velocity of 60,000 h-1, and NOxconversion of 2V2Cu8Mn/TiO2reached nearly 100% at 275~325 °C. The X-ray diffraction characterization showed that the CuO and Mn2O3phases in VCuMn/TiO2catalysts were the main active site and with better SCR activity at middle-low temperature. In addition, sulfur-resistance of VCuMn/TiO2catalysts were investigated, NOxconversion on 2V2Cu8Mn/TiO2was above 60% at 250 °C after feeding SO2(600 ppm). It can be concluded that the modified VCu/TiO2catalyst by adding manganese obtained a good de-NOxactivity in a high sulfur content gas stream.
Physical Characterisation of Millet Starch during Roasting
Authors: Hui Ming , Shang Xiao Li , Tian Qing , Zhang Kai Ping
Published: July 2012

The gelatinization and liquefaction properties of roasted millet starch were investigated in this study. Results showed that the particle morphology of roasted millet starch greatly changed as compared with control, and its solubility and swelling degree increased with temperature increment, in which the former increased rapidly above 70°C and was basically stable after 80°C, while the latter was overall lower than the raw millet starch's. The viscosity of millet starch paste increased with decreasing temperature, but that of the roasted starch increased more slowly than that of the raw. There was little effect of pH value on viscosity of roasted millet starch. Compared with the raw, the roasted millet starch would be firstly reached scheduled DE value under the same conditions, which could reduce liquefaction time in the course of production.
Swelling Properties and Kinetics of Starch-g-Poly(acrylic acid) Hydrogels
Authors: Shi Ji Yan , Fan Li Ren , Song Ji Qing , Bai Wen Bo
Published: July 2012

Starch-g-poly(acrylic acid) (CSt-g-PAA) hydrogels were prepared from cassava starch and acrylic acid by graft polymerization. The hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric-differential scanning calorimetry (TG-DSC). Additionally, the water absorbency and hygroscopic swelling properties of the hydrogels were investigated respectively. The two processes were simulated using mathematical modes. The results revealed that the crystal structure of starch was destroyed. At 40°C and relative humidity 90% (90% RH), the samples (starch mass15%, 50%) have a better performance of the moisture absorption. Hygroscopic rate declined with the increase of relative humidity.
Studies on Antioxidant Activity of Cherry Tomatoes Wine
Authors: Xiao Chun Ling , Li Hong Ying , Zhang Zhen , Liang Rui Feng
Published: July 2012

Using cherry tomatoes as raw materials, the experiment studies its antioxidant activity in different fermentation conditions . By measuring the content of flavonoid,and polyphenols, clearance to DPPH •and OH• , and reducing power of cherry tomatoes wine in different fermentation conditions, theantioxidation capability can be judged. The results show that content of alcohol achieves 16 °, concentration of SO2 is110 mg/L, fermentation time is between3 d and 4 d, the antioxidant ability of cherry tomato fruit wine is optimum.
Microbial Prevention of Wax Deposition in Crude Oil
Authors: Liu Jiong Hong , Jia Yun Peng , Xu Rui Dan
Published: July 2012

Microorganisms were obtained by separation and purification experiment from waxy oil production wells in Daqing Oilfield. The paraffin removal strain was named for S1, and the biological surfactant strain was named for G1. Microscopic and morphological examinations showed strain S1 was to be Bacillus Subtilis and strain G1 was to be Bacillus Cereus. As an indicator of the degradation of paraffin, strain S1 and strain G1 were added in different proportions, the optimum proportion was 5:2. In this proportion the degradation rate of paraffin could reach to 64%, the prevention rate of paraffin could reach to 55%. By experiment after mixed bacteria group treatment, the viscosity of crude oil reduced from 36.9mPa•s to 27.8mPa•s, the reduction rate of viscosity was 24.7%, and the freezing point of crude oil reduced by 3.6°C, paraffinic hydrocarbons of crude oil were degraded.
Gel Breaking Affecting Factors Analysis on Polymer Bio-Degradation
Authors: Cai Ji Hua , Gu Sui , Liu Hao
Published: July 2012

Recent years have witnessed a renewed interest in the development of coalbed methane (CBM) reservoirs. However, horizontal drilling in unconsolidated and soft coal seam for CBM drainage and exploitation often results in wellbore instability. This paper proposed degradable drilling fluid in CBM horizontal drilling which not only can maintain wellbore stability, but also minimize formation damages caused by drilling fluid. Its factors of gel breaking ratio were systematically studied from the aspects as substrate concentration, pH, temperature, enzyme concentration and reaction time. We found that in a condition when enzyme concentration is abundant, increasing of substrate concentration would result in higher gel breaking ratio. Furthermore, nearly neutral pH (pH≈7), moderate temperature (40°C~60°C), enzyme with higher concentration and longer action time (more than 3 hours) could enhance gel breaking ratio.
Nanocomposite of LaVO4/TiO2 as a Wide Spectrum Responsive Photocatalyst for Degradation of Volatile Organic Compounds in the Gas Phase
Authors: Huang Han Jie
Published: July 2012

In this paper, a wide spectrum responsive LaVO4/TiO2 nanocomposite has been characterized by Laser Raman and X-ray photoelectron spectra (XPS). Strong photocatalytic activities of LaVO4/TiO2 nanocomposite towards the removal of gaseous volatile organic pollutants (typically aromatic compounds) under visible light irradiation were demonstrated. Moreover, applications were also given for the photocatalytic elimination of gaseous benzene under simulated solar light and UV light irradiation.
Efficient Production of Optical Pure D-Lactate by HBUT-C Engineered Escherichia coli W
Authors: Wang Yong Ze , Tian Tian , Zhao Jin Fang , Zhao Xiao , Wang Jin Hua , Zhou Sheng De
Published: July 2012

HBUT-C,engineered by Escherichia coli W, was constructed as a new biocatalyst for the production of D-lactate.The strain can grow well in mineral salts medium.HBUT-C completed the fermentation of 10% glucose in 64h to produce 834 mmol·L-1lactate of fermentation broth with volumetric productivity of 1.16g·L-1·h-1. The optical purity of D-lactate was higher than 98.3%. It was also found that HBUT-C with inactivate genes encoding competing pathways produced 58.9 mM acetate, traces of formate and succinate by-product under anaerobic conditions.
Homogeneous Modification of Sugarcane Bagasse Cellulose with 2-(Dimethylamino)ethylmethacrylate in Ionic Liquid under Microwave Irradiation
Authors: Chang Gang , Wei Xiao Yi , Li Ji Hua , Zhang Cheng Hui , Chen Jia Cui
Published: July 2012

Abstract: A cellulose grafted copolymer was synthesized by homogeneous grafting 2-(Dimethylamino)ethylmethacrylate (DMAEMA) onto the sugarcane bagasse cellulose under microwave irradiation. Cellulose was dissolved in 1-butyl-3-methylinidazolium chloride ionic liquid ([Bmim]Cl) to form a homogeneous solution. The grafting polymerization was then initiated by potassium persulfate in the presence of N,Nˊ-methylenebisacrylamide as a crosslinker. Compared with the traditional thermal heating method, microwave irradiation had a drastic reduction of reaction time and an increased grafted efficiency. The characterization of the graft copolymer was confirmed by Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscope (SEM), and Thermogravimetric analysis (TGA). Moreover, the homogenous reaction media applied can be carried out in completely recycled ionic liquid, and the grafted cellulose products have a potential application in wastewater anion absorption.
Preparation and Identification of Monoclonal Antibody against BPA
Authors: Zhang Lei , Zhao Su Qing , Huang Hong
Published: July 2012

Firstly, BPA structure was modified, then coupling BPA with BSA or OVA to prepare immunogen and coating antigen. Five Balb/C mice were immunized with BPA-BSA. Finally an antibody was prepared and the indirect competitive enzyme-linked immunosorbent assay was founded. Results:(1) The monoclonal antibody belongs to IgG1 subtype and К light chain.(2) The antibody titer is 1：256000, the most suitable concentration of coating antigen is 2μg/mL, and the optimal dilution of antibody and HRP are 1:16000 and 1:10000 respectively. (3)The linear regression line equation is y = 0.1139x + 0.1046, correlation coefficient is R2=0.97, the detection limit is 0.911ng/mL and IC50 is 2.454×103ng/mL. (4)The monoclonal antibody has high specificity for the cross reactivity with phenol, hydroquinone, and tert-butyl hydroquinone being lower than 0.01%, except ortho-hydroxybenzoic acid 2.1%. (5)The recovery range is 93%～116% and 89%～112% when adding BPA into black samples.（6）When the method was used in real materials to detect BPA residual, the results were proximate to the dates by HPLC.
Validation of Fermentative Parameters for Efficient Succinate Production in Batch Operation by Actinobacillus succinogenes 130ZT
Authors: Sawisit Apichai , Seesan Supaluk , Chan Sitha , Kanchanatawee Sunthorn , Jantama Sirima Suvarnakuta , Jantama Kaemwich
Published: July 2012

Succinate is an important platform molecule in the synthesis of a number of commodity and specialty chemicals. In the present study, the effects of different carbon and nitrogen sources, initial pH of the growth medium (pH 4.5-9.0), and temperature (25-45°C) on the fermentative succinate production by Actinobacillus succinogenes 130ZT were investigated in 100 mL anaerobic bottles. The results revealed that the highest concentration of succinate at 6.28 g/L was produced from 10 g/L of glucose or lactose in the medium containing 5 g/L yeast extract at 24 h. However, a comparable concentration of succinate was also produced when the medium was supplemented with 5 g/L spent brewer’s yeast extract. Based on these results, the cost effectiveness of succinate production could be improved by the use of glucose or lactose fermentation supplemented with spent brewer’s yeast extract. Optimized initial pH at 8.0, temperature at 37 °C, and inoculum size at 6% (v/v) provided the best succinate production at the concentration of 6.37 g/L with a yield of 68.73%.
Study on Catalytic Decomposition of N2O over Modified Zeolites
Authors: Liu Xing Yong , Wei Wei
Published: July 2012

A series of Beta, ZSM-5 and MCM-22 zeolites modified with metal ions (such as Fe 3+ , Co 2+ , Ce 3+ , Ni 2+ and Cu 2+ ) for catalytic decomposition of N2O were prepared by using wet ion exchange method. The effects of different metal ions, the contents of metal ions and silicon aluminum ratio on catalytic decomposition activities of N2O were investigated, respectively. The results indicated that Beta with a SiO2/Al2O3=30 and 1% Fe 3+ have better catalytic decomposition activities to N2O, the completed catalytic decomposition temperature to 35% N2O in the condition of 4000 h-1 GVSH is 350 0 C.
Screening and Influencing Factors of Halophilic Denitrifying Bacteria
Authors: Tang Jing , E Hong Ming , Fu Jin Xiang , Chen Jin Nan , Fan Ming
Published: July 2012

With nitrite or nitrate nitrogen as electron acceptor in the high salt conditons, halophilic denitrifying bacteria can transfer nitrite or nitrate to nitrogen, thereby purifying the high-salt wastewater. Halophilic denitrifying bacteria play an important role in the carbon and nitrogen removal of saline wastewater, such as petroleum, chemical industry, seafood processing and seafood farming. This article dissussed halophilic denitrifying bacteria screening, the main types and the corresponding morphological characteristics, then we focused on the research progress of main factors of halophilic denitrifying bacteria’s growth and nitrogen removal. Finally put forward the current problems of the research and development trend of halophilic denitrifying bacteria.
Comparison of 4,4,4-Trifluoro-3-(indole-3-)butyric Acid Dissolved in Water and Dimethyl Sulfoxide as a Solvent on Root Growth of Rice
Authors: Li Xiu Ping , Zhang Yan Lai , Hideo Sasakawa
Published: July 2012

The effective dissolution method of 4,4,4-trifluoro-3-(indole-3-)butyric acid (TFIBA) to rice plant growth was examined. Elongation of rice seminal root was promoted approximately 80% by continuous supply of 10-5 mol L-1 TFIBA dissolved in water. The primitive effect of TFIBA dissolved in DMSO on root elongation was greater than that of TFIBA dissolved in water, and the length of root was significantly increased by 233% by treatment with TFIBA at 10-5 mol L-1, compared with that in untreated control seedlings. However, DMSO occurred increase of root weight and showed somewhat disadvantage about reproducibility of TFIBA effect. In contrast, the reproducibility of TFIBA effect was high in the solution prepared with water.
Purification of Indoor Formaldehyde Using Ornamental Plant of Kafirlily
Authors: Zheng Wen Xia , Chen Ying Ming , Huang Ying
Published: July 2012

In order to solve the indoor air pollution caused by formaldehyde, an ornamental plant of Kafirlily was used to purify the formaldehyde in this paper. By testing the absorbing effect on ornamental in the transparent airtight cabin, the physiological and biochemical parameters such as leaf hurt index and peroxidase (POD) activity were determined. The experimental results showed that the Kafirlily can decline the formaldehyde concentration to a certain degree, the leaves were hurt little, and the POD activity changed markedly. The experiments can indicate that Kafirlily can purify the indoor formaldehyde in certain concentration range with strong tolerant ability and high POD physiological stress responses.
Adsorptive Properties of the Preparation Material from the Skin of Corn Stalk for Enzyme Immobilization
Authors: Lv Jin Shun , Shen Tian Tian , Nie Li
Published: July 2012

In order to investigate the characterizations of skin adsorption material (SAD) prepared from skin of corn stalks by High Boiling Solvent treatment, neutral proteinase and maltogenic amylase were selected as adsorbate to assess its potential applicability for enzyme immobilization. The structure of SAD and Skin were compared by FT-IR and XRD. The characterizations of the adsorption of the different enzymes onto SAD were studied. The isotherm of the maltogenic amylase was found to be linear with the Freundlich equation, and proteinase was found to be linear with the Langmuir equation in the studied concentration range. SAD could be applied the bio-active material and Skin to prepare SAD.
Synthesis and Photocatalytic Mechanism of Visible Light-Activated LaVO4/TiO2 Composite
Authors: Huang Han Jie , She Wen Long , Yang Ling Wen
Published: July 2012

In this work, a visible light-activated LaVO4/TiO2 composite photocatalyst has been successfully synthesized via a facile coupled method. The composite was characterized by powder X-ray diffraction (XRD), UV-vis diffuse reflectance spectroscopy (DRS) and spin-trapping electron paramagnetic resonance (EPR). Based on the detection of active oxygen species, a visible light-induced photocatalytic degradation mechanism of benzene on LaVO4/TiO2 was proposed.
Improved Production of 1-Deoxynojirmiycin via Mixed Cultivation of Bacillus subtilis and Fungi Isolated from Mulberry Rhizosphere
Authors: Huang Qian , Li Qiang , Ji Geng Sheng , Fan Ting Lu , Gu Xu Ding , Zhai Lin Zhi , Wang Hui Ping , Liu Yang , Fei Juan Juan
Published: July 2012

To help meet the challenge of 1-Deoxynojirmiycin (DNJ) manufacture from microbial fermentation, optimal condition for the fermentation has attracted substantial research interest. In the case, mix strategy was carried out to cultivate mulberry rhizosphere Bacillus Subtilis BJ-B121 (DNJ producing strain), Rhizopus spp.BJ-F13 and Aspergillus niger BJ-F8 according to the plate dual culture assay and inhibitory activity assay in vitro. We characterized mixed cultivation of Bacillus Subtilis BJ-B121 and mulberry rhizosphere microorganisms on the basis of biochemical data. Rhizosphere soil microorganisms may interact with each other and lead to improvement of DNJ production. Campared with mono culture, the glucose consumption and DNJ production were efficient. DNJ production was improved by 26-32% in mixed cultivation, 100 μg/mL and 308 μg/mL extra DNJ production were obtained. In addition, DNJ did not affect the growth of the mixed cells.
Synthesis of Hydroxypropyl Dioscorea alata Starch Rapidly Prepared with Ultrasonic-Microwave Assistance
Authors: Sheng Ling Zhi , Wu Song Hai , Zhang Hai De , Jiang Xin Xin
Published: July 2012

In this study, ultrasonic-microwave assisted synthesis of the hydroxypropyl Dioscorea alata starch was investigated and optimized. Under the irradiation of ultrasonic-microwave, Dioscorea alata starch was added to the reactor as raw material, and five factors (propylene oxide, sodium hydroxide, anhydrous sodium sulfate, microwave treatment time and microwave power) that influence the preparation of hydroxypropyl Dioscorea alata starch were analyzed using single-factor and orthogonal experiment methodology. Characteristic of products was evaluated by determining their molar substitution (MS). During the experiment , ultrasonication power was fixed at 50 w. The optimum conditions were determined as propylene oxide 12% (w/w), sodium hydroxide 1.0% (w/w), anhydrous sodium sulfate 8% (w/w), microwave irradiation time 110 s and microwave power 80 w. The optimal condition of the product gives a MS value at 0.82. The hydroxypropyl Dioscorea alata starch paste shows higher light transmittance(25.5%) and solubility in cold water, lower gelatinization temperature(59°C) in comparison to native starch. Compared with conventional process, the reaction time was significantly shortened by ultrasonic-microwav
Study on a New High-Efficiency Treatment Method of Printing and Dyeing Wastewater
Authors: Li Qin , Lin Tao , Li Fu Bao
Published: July 2012

The large quantity of dyeing and printing wastewater, which contains high organics which can be hardly decomposed, is one of the main sources of water pollution. Based on summarizing the existing problems of traditional processing methods, the technology of cavitation jet and impinging streams were organically combined, which has great potential in wastewater treatment field, as a new method for degrading printing and dyeing wastewater, and then the “cavitation impinging streams (CIS)” concept was proposed, in which the superior features in wastewater degradation with CIS was expounded, and the problems to be further studied were pointed out.
Comparison Study of Amino-Functionalized and Mercaptopropyl-Functionalized Mesoporous Silica MCM-41
Authors: E. Sangok Faustina , M. Yahaya Sabrina , Nurul Izza Taib , Sa'ad Siti Zaleha , Rasaruddin Nor Fazila
Published: July 2012

The ability to decorate silicate surface with different organoalkoxysilanes creates powerful new capabilities for catalyst, adsorbents and chemical separation. Mesopororus silica, MCM-41 was modified by grafting of amino and mercaptopropyl functional group. The structures of these materials were characterized by using Fourier Transform Infrared Spectroscopy (FT-IR), and X-Ray diffraction (XRD). The samples were found to exhibit structural properties similar to those reported earlier. Significant functional groups of the modified mesoporous silicates were found in the spectrum of FT-IR. Standard structure of mesoporous silicates were found to be preserved at planar [100] of XRD difractogram of mesoporous silicates. Adsorption of Cu (II) ions were done under different temperatures, initial concentrations and pH. Adsorption process also was determined from kinetic point of view and was found to be better fitted to pseudo second order of kinetic model.
Study on Technic Condition of Extracting Solanesol from Abandon Tobacco
Authors: Yang Mei , Gu Zheng Gui
Published: July 2012

Solanesol is an important pharmaceutical intermediate. In this paper, organic solvent is used to extract solanesol from the abandoned tobacco. The conditions of extracting the solanesol are optimized, such as extraction solvent, solvent ratio, extraction temperature and time. The extraction times are also identified. L9(34) design is selected to investigate the factors above. Finally, the optimal extraction conditions are proposed. The petroleum ether is used as solvent, the solvent ratio is 15:1(volume of solvent is 15ml, quality of leaves is 1g), the extraction temperature is 50°C and the time is 4h. The purity of solanesol reaches to 57.56% and the total yield reaches to 6.39%.