bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Throughput Sequencing Reveals the Regulatory Networks of Transcriptome and Small RNAs During the Defense Against Marssonina brunnea in Poplar
Authors: Liao Yangwenke , Zhang Qingyue , Cui Rongrong , Xu Xin , Zhu Fuyuan , Cheng Qiang , Li Xiaogang
Published: September 2021

MicroRNAs are implicated in the adjustment of gene expression in plant response to biotic stresses. However, the regulatory networks of transcriptome and miRNAs are still poorly understood. In the present study, we ascertained the induction of genes for small RNA biosynthesis in poplar defense to a hemibiotrophic fungus Marssonina brunnea and afterward investigated the molecular regulatory networks by performing comprehensive sequencing analysis of mRNAs and small RNAs in M. brunnea-inoculated leaves. Differentially expressed genes in M. brunnea-infected poplar are mainly involved in secondary metabolisms, phytohormone pathways, the recognition of pathogens, and MAPK pathway in the plant, with real-time quantitative PCR (qPCR) validating the mRNA-seq results. Furthermore, differentially expressed miRNAs, such as MIR167_1-6, MIR167_1-12, MIR171_2-3, MIR395-13, MIR396-3, MIR396-16, MIR398-8, and MIR477-6, were identified. Through psRobot and TargetFinder programs, MIR167-1-6, MIR395-13, MIR396-3, MIR396-16, and MIR398-8 were annotated to modulate the expression of genes implicated in transportation, signaling, and biological responses of phytohormones and activation of antioxidants for plant immunity. Besides, validated differentially expressed genes involved in lignin generation, which were phenylalanine ammonia-lyase, ferulate-5-hydroxylase, cinnamyl alcohol dehydrogenase, and peroxidase 11, were selected as targets for the identification of novel miRNAs. Correspondingly, novel miRNAs, such as Novel MIR8567, Novel MIR3228, Novel MIR5913, and Novel MIR6493, were identified using the Mireap online program, which functions in the transcriptional regulation of lignin biosynthesis for poplar anti-fungal response. The present study underlines the roles of miRNAs in the regulation of transcriptome in the anti-fungal response of poplar and provides a new idea for molecular breeding of woody plants.
Arabidopsis CALMODULIN-LIKE 38 Regulates Hypoxia-Induced Autophagy of SUPPRESSOR OF GENE SILENCING 3 Bodies
Authors: Field Sterling , Conner William Craig , Roberts Daniel M.
Published: September 2021

During the energy crisis associated with submergence stress, plants restrict mRNA translation and rapidly accumulate stress granules that act as storage hubs for arrested mRNA complexes. One of the proteins associated with hypoxia-induced stress granules in Arabidopsis thaliana is the calcium-sensor protein CALMODULIN-LIKE 38 (CML38). Here, we show that SUPPRESSOR OF GENE SILENCING 3 (SGS3) is a CML38-binding protein, and that SGS3 and CML38 co-localize within hypoxia-induced RNA stress granule-like structures. Hypoxia-induced SGS3 granules are subject to turnover by autophagy, and this requires both CML38 as well as the AAA+-ATPase CELL DIVISION CYCLE 48A (CDC48A). CML38 also interacts directly with CDC48A, and CML38 recruits CDC48A to CML38 granules in planta. Together, this work demonstrates that SGS3 associates with stress granule-like structures during hypoxia stress that are subject to degradation by CML38 and CDC48-dependent autophagy. Further, the work identifies direct regulatory targets for the hypoxia calcium-sensor CML38, and suggest that CML38 association with stress granules and associated regulation of autophagy may be part of the RNA regulatory program during hypoxia stress.
The Influence of Health Literacy and Social Support on Loneliness Among Patients With Severe Mental Illness in Rural Southwest China
Authors: Liu Yan , Meng Hongdao , Conner Kyaien O , Qiao Mutian , Liu Danping
Published: September 2021

Loneliness is an important risk factor for poor health outcomes among adults, especially among those with severe mental illnesses (SMIs). Existing research has shown that adults with SMIs often lack health literacy, which contributes to more restricted social networks and low levels of social support. The objective of this cross-sectional study was to examine the influence of health literacy and social support on the loneliness of patients with SMI in rural Southwest China. We recruited 300 patients with SMI in rural Southwest China between December, 2017 to May, 2018 via a multi-stage stratified random sampling approach. We used structural equation modeling (SEM) test the hypothesized relationships among the variables of the 270 patients who completed the survey. Results of the SEM showed that health literacy was both directly and indirectly associated with loneliness, with social support playing a mediating role. These findings suggest psychoeducation for SMI patients, and their informal caregivers, may offer beneficial effects toward reducing loneliness in this vulnerable population. Further, social support is another potential target for intervention development for improving patient outcomes.
Facilitating Adaptive Emotion Processing and Somatic Reappraisal via Sustained Mindful Interoceptive Attention
Authors: Price Cynthia J. , Weng Helen Y.
Published: September 2021

Emotions are by nature embodied, as the brain has evolved to quickly assess the emotional significance of stimuli and output signals to the body’s viscera and periphery to aid adaptive responses. Emotions involve both implicit bodily and explicit narrative processes, and patients may experience transdiagnostic distress when bodily signals are not attended to and holistically integrated with explicit narratives about experience. Similarly, therapists may be trained in more implicit body-based approaches (i.e., massage/bodywork, physical and occupational therapy, and nursing/medicine) or more explicit narrative-based approaches (i.e., psychotherapy), and may lack training in skills that integrate both levels of emotion processing to aid healing and growth. To address these gaps, we propose a framework where the bridge between implicit bodily sensations and explicit narratives lies in cultivating mindful awareness of bodily sensations associated with emotions. This process brings subjective awareness to notice inner body experience (or interoceptive awareness) that is often outside of conscious awareness, so that it may be understood and re-integrated in more adaptive ways, which we call somatic reappraisal. Using clinical theory and example vignettes, we present mindful interoceptive awareness for adaptive emotion processing as a framework to cultivate and enhance somatic reappraisal. Mindful interoceptive awareness brings more focused and sustained attention to inner body experience; likewise, internal sensations associated with emotions become more granular, vivid, and can shift in ways that facilitate somatic reappraisal. Learning to sustain interoceptive awareness when engaged with mindfulness qualities of nonjudgment and compassion promotes an experience where new associations between emotions, meanings, and memories can be made that generate insights that are holistic and integrative. A clinical vignette is used in this paper to provide examples of this approach in psychotherapy. An example script for use in mindfulness groups is included, and resources are suggested for clinicians to gain more experience. Mindful interoceptive awareness for adaptive emotion processing is a clinical process that can be learned and applied by a range of clinicians to treat mental and physical health conditions that may benefit greater embodied awareness.
A Perceived Zone of Certainty and Uncertainty: Propositions for Research Development
Authors: Phan Huy P. , Ngu Bing H.
Published: September 2021

Possessing expert schemas is a positive feat that may yield different types of adaptive outcomes (e.g., informing procedural understanding that may result in a student skipping a few of the solution steps involved). Limited schemas, in contrast, may deter progress of a novice learner, limiting his/her capability to flourish. Taken as a whole, it may be concluded that expert schemas are more advantageous than novice schemas, differentiating learners in terms of expert and novice. Having said this, however, more recently, researchers have argued that possessing expert schemas could serve as deterrence. Recently, researchers have acknowledged a theoretical concept known ascognitive entrenchment, which is defined as a high level of stability in domain schemas. This description interestingly suggests that “entrenchment” or “situated fixation” of a course of action (e.g., a subject matter) could hinder the progress and learning experience of a person, namely—his/her inability and/or unwillingness to adapt to a new context, and/or his/her inflexibility and insistence to stay on course without any intent to change. One example of cognitive entrenchment is observed in professional football, wherein it has been argued that some football coaches are cognitively entrenched within their expert schemas, resulting in their demised game plans and strategic acumen. We advance the study of cognitive entrenchment by proposing an alternative viewpoint, which we term as the “perceived zone of certainty and uncertainty.” This proposition counters the perspective of cognitive entrenchment by arguing that it is cognitive appraisal, judgment, mental resolute, and determination of a person incognitive certaintyof his/her success or failure, or thecognitive uncertaintyof success or failure, that would explain the notion of inflexibility and/or unwillingness to adapt, and/or insistence to stay on course without any attempt to deviate. Moreover, we rationalize that certainty of success or failure would closely associate with a feeling of comfort, whereas uncertainty would associate with his/her feeling discomfort. In this analysis, we strongly believe that willingness to change and adapt, reluctance and insistence to remain on course, and/or inclination to embrace flexibility may not necessarily relate to the concept of cognitive entrenchment; rather, inflexibility and/or reluctance to change for the purpose of adaptation has more to do with the desire of a person to seek a state of comfort. Finally, our conceptual analysis of cognitive entrenchment also considers an interesting theoretical concept, which we termed as “perceived optimal efficiency.” Perceived optimal efficiency, similar tocognitive relevance theory, is concerned with the relationship between minimum investment of time, effort, cognitive resources, etc., and an optimal best outcome. The issue for discussion, from our point of view, is related to the extent to which the certainty of success or failure would associate with perceived optimal efficiency.
Meaning-Making of Motherhood Among Mothers With Substance Abuse Problems
Authors: To Siu-ming , Yan Ming-wai , Lau Cheryl Danielle
Published: September 2021

Previous literature has documented the unique challenges encountered by mothers with substance abuse problems, which may hinder the ability to fulfill parenting responsibilities. Since there is evidence suggesting the engagement in meaning-making processes can help individuals reinterpret their transitions into parenthood and cope with parental stress, this study examined the meaning-making processes of motherhood among mothers with substance abuse problems. Sixteen Hong Kong Chinese mothers with a history of substance abuse were purposively selected and invited to narrate their life and maternal experiences in individual interviews. Based on the meaning-making model in the context of stress and coping, whereby global meaning refers to orienting system of an individual and situational meaning refers to the meaning one attributes to a particular situation, the global and situational meanings of participants related to motherhood and substance use, and their reappraised meanings in response to the discrepancies between global and situational meanings were analyzed. Using thematic analysis, the results showed that when faced with an internal conflict between global and situational meanings induced by substance abuse, most participants engaged in the meaning-making process of assimilation. Rather than changing their inherent parental beliefs and values, most participants adjusted their appraisals toward the situation, and hence made changes in their cognitions or behaviors such as making efforts to quit substance use or reprioritizing their parenting responsibilities. The analysis further revealed that being a mother provided a significant source of meaning to the participants in confronting highly stressful mothering experiences induced by substance abuse. Altogether, the findings suggest that a meaning-making approach may have benefits and implications for helping this population reorganize their self-perceptions, gain a clearer sense of future direction in motherhood, and achieve more positive life and parenting outcomes.
The Words of Affectivity. Affect, Category, and Social Evaluation Norms for 400 Polish Adjectives
Authors: Grzybowski Szczepan J. , Wyczesany Miroslaw , Cichecka Hanna , Tokarska Aleksandra
Published: September 2021

Emotional adjectives can be grouped into two main categories: denoting and connoting stable (personality) traits and denoting and connoting transient (mood) states. They relate closely to the concept of affectivity, which is a pervasive tendency to experience moods of positive or negative valence. They constitute a rich study material for personality and affect psychology and neuroscience. Thus, this study was designed to establish a normed list of emotional adjectives with ratings encompassing four dimensions: emotional valence (positive or negative), emotional arousal (low-arousing or high-arousing), category (state, trait, and hybrid), and social judgment (competence, morality, and mixed). The adjectives were preselected based on previous broad Polish norming studies, personality and mood questionnaires, and a dictionary study. The results of the study were drawn from 195 participants who rated 400 adjectives that were chosen based on similar linguistic variables, such as frequency and word length. The dataset measures were proven to be stable and reliable. Correlations between the emotional valence and state-trait, valence and competence-morality, and emotional arousal and competence-morality dimensions were found. The study was successful in preparing a dataset of well-categorized (state, trait, and hybrid) positive and negative adjectives of moderate to high arousal ratings. Since the words were matched on linguistic variables, the set provided useful material that can be readily used for research into the effects of the category and emotional dimensions on language processing and as a basis for new personality questionnaires and mood checklists. The dataset could also be seen as a supplement for broader sets of published normed materials in Polish that link emotion and language.
People With High Autistic Traits Show Fewer Consensual Crossmodal Correspondences Between Visual Features and Tastes
Authors: Chen Na , Watanabe Katsumi , Wada Makoto
Published: September 2021

Crossmodal correspondences between visual features (e.g., color/shape) and tastes have been extensively documented in recent years. Visual colors and shapes have been shown to consensually match to specific tastes. Meanwhile, individuals with autism spectrum disorder are reported to have atypical sensory processing and deficits in multisensory integration. However, the influence of autistic traits on the formation of such correspondences is relatively unknown. Here, we examined whether autistic traits could influence visual–taste associations using an online questionnaire survey among Japanese participants. The results showed that the participants exhibited strong color–taste, shape–taste, and shape–color associations, and the proportions of choosing the consensual color–taste/shape–color associations were significantly associated with autistic traits. The participants with higher autistic quotient scores chose fewer of the consensual color–taste/shape–color associations while there was no difference in choosing shape–taste associations. We interpreted the results as statistical learning with a reduced prior knowledge effect in participants with higher autistic quotient scores.
Service User Experiences of How Flexible Assertive Community Treatment May Support or Inhibit Citizenship: A Qualitative Study
Authors: Brekke Eva , Clausen Hanne K. , Brodahl Morten , Lexén Annika , Keet Rene , Mulder Cornelis L. , Landheim Anne S.
Published: September 2021

The aim of this study was to explore and describe service user experiences of how receiving services from a Flexible Assertive Community Treatment (FACT) team may support or inhibit citizenship. Within a participatory design, individual interviews with 32 service users from five Norwegian FACT teams were analyzed using thematic, cross-sectional analysis. The findings showed that FACT may support citizenship by relating to service users as whole people, facilitating empowerment and involvement, and providing practical and accessible help. Experiences of coercion, limited involvement and authoritarian aspects of the system surrounding FACT had inhibited citizenship for participants in this study.
Bidirectional Behavioral Selection in Mice: A Novel Pre-clinical Approach to Examining Compulsivity
Authors: Mitra Swarup , Bult-Ito Abel
Published: September 2021

Obsessive-compulsive disorder (OCD) and related disorders (OCRD) is one of the most prevalent neuropsychiatric disorders with no definitive etiology. The pathophysiological attributes of OCD are driven by a multitude of factors that involve polygenic mechanisms, gender, neurochemistry, physiological status, environmental exposures and complex interactions among these factors. Such complex intertwining of contributing factors imparts clinical heterogeneity to the disorder making it challenging for therapeutic intervention. Mouse strains selected for excessive levels of nest- building behavior exhibit a spontaneous, stable and predictable compulsive-like behavioral phenotype. These compulsive-like mice exhibit heterogeneity in expression of compulsive-like and other adjunct behaviors that might serve as a valuable animal equivalent for examining the interactions of genetics, sex and environmental factors in influencing the pathophysiology of OCD. The current review summarizes the existing findings on the compulsive-like mice that bolster their face, construct and predictive validity for studying various dimensions of compulsive and associated behaviors often reported in clinical OCD and OCRD.
Predictive Models of Maternal Harsh Parenting During COVID-19 in China, Italy, and Netherlands
Authors: Riem Madelon M. E. , Lodder Paul , Guo Jing , Vrielink-Verpaalen Michelle , van IJzendoorn Marinus H. , Bakermans-Kranenburg Marian J. , De Carli Pietro
Published: September 2021

Background: The COVID-19 pandemic drastically impacted on family life and may have caused parental distress, which in turn may result in an overreliance on less effective parenting practices.

Objective: The aim of the current study was to identify risk and protective factors associated with impaired parenting during the COVID-19 lockdown. Key factors predicting maternal harsh discipline were examined in China, Italy, and the Netherlands, using a cross-validation approach, with a particular focus on the role of allomaternal support from father and grandparents as a protective factor in predicting maternal harshness.

Methods: The sample consisted of 900 Dutch, 641 Italian, and 922 Chinese mothers (age M = 36.74, SD = 5.58) who completed an online questionnaire during the lockdown.

Results: Although marital conflict and psychopathology were shared risk factors predicting maternal harsh parenting in each of the three countries, cross-validation identified a unique risk factor model for each country. In the Netherlands and China, but not in Italy, work-related stressors were considered risk factors. In China, support from father and grandparents for mothers with a young child were protective factors.

Conclusions: Our results indicate that the constellation of factors predicting maternal harshness during COVID-19 is not identical across countries, possibly due to cultural variations in support from fathers and grandparents. This information will be valuable for the identification of at-risk families during pandemics. Our findings show that shared childrearing can buffer against risks for harsh parenting during COVID-19. Hence, adopting approaches to build a pandemic-proof community of care may help at-risk parents during future pandemics.
Vascular Aging in Rodent Models: Contrasting Mechanisms Driving the Female and Male Vascular Senescence
Authors: Barros Paula R. , Costa Tiago J. , Akamine Eliana H. , Tostes Rita C.
Published: September 2021

Increasing scientific interest has been directed to sex as a biological and decisive factor on several diseases. Several different mechanisms orchestrate vascular function, as well as vascular dysfunction in cardiovascular and metabolic diseases in males and females. Certain vascular sex differences are present throughout life, while others are more evident before the menopause, suggesting two important and correlated drivers: genetic and hormonal factors. With the increasing life expectancy and aging population, studies on aging-related diseases and aging-related physiological changes have steeply grown and, with them, the use of aging animal models. Mouse and rat models of aging, the most studied laboratory animals in aging research, exhibit sex differences in many systems and physiological functions, as well as sex differences in the aging process and aging-associated cardiovascular changes. In the present review, we introduce the most common aging and senescence-accelerated animal models and emphasize that sex is a biological variable that should be considered in aging studies. Sex differences in the cardiovascular system, with a focus on sex differences in aging-associated vascular alterations (endothelial dysfunction, remodeling and oxidative and inflammatory processes) in these animal models are reviewed and discussed.
Physics-Based Modelling and Simulation of Multibeam Echosounder Perception for Autonomous Underwater Manipulation
Authors: Choi Woen-Sug , Olson Derek R. , Davis Duane , Zhang Mabel , Racson Andy , Bingham Brian , McCarrin Michael , Vogt Carson , Herman Jessica
Published: September 2021

One of the key distinguishing aspects of underwater manipulation tasks is the perception challenges of the ocean environment, including turbidity, backscatter, and lighting effects. Consequently, underwater perception often relies on sonar-based measurements to estimate the vehicle’s state and surroundings, either standalone or in concert with other sensing modalities, to support the perception necessary to plan and control manipulation tasks. Simulation of the multibeam echosounder, while not a substitute for in-water testing, is a critical capability for developing manipulation strategies in the complex and variable ocean environment. Although several approaches exist in the literature to simulate synthetic sonar images, the methods in the robotics community typically use image processing and video rendering software to comply with real-time execution requirements. In addition to a lack of physics-based interaction model between sound and the scene of interest, several basic properties are absent in these rendered sonar images–notably the coherent imaging system and coherent speckle that cause distortion of the object geometry in the sonar image. To address this deficiency, we present a physics-based multibeam echosounder simulation method to capture these fundamental aspects of sonar perception. A point-based scattering model is implemented to calculate the acoustic interaction between the target and the environment. This is a simplified representation of target scattering but can produce realistic coherent image speckle and the correct point spread function. The results demonstrate that this multibeam echosounder simulator generates qualitatively realistic images with high efficiency to provide the sonar image and the physical time series signal data. This synthetic sonar data is a key enabler for developing, testing, and evaluating autonomous underwater manipulation strategies that use sonar as a component of perception.
A 3D-Printable Robotic Gripper Based on Thick Panel Origami
Authors: Liu Chenying , Maiolino Perla , You Zhong
Published: September 2021

Origami has been a source of inspiration for the design of robots because it can be easily produced using 2D materials and its motions can be well quantified. However, most applications to date have utilised origami patterns for thin sheet materials with a negligible thickness. If the thickness of the material cannot be neglected, commonly known as the thick panel origami, the creases need to be redesigned. One approach is to place creases either on top or bottom surfaces of a sheet of finite thickness. As a result, spherical linkages in the zero-thickness origami are replaced by spatial linkages in the thick panel one, leading to a reduction in the overall degrees of freedom (DOFs). For instance, a waterbomb pattern for a zero-thickness sheet shows multiple DOFs while its thick panel counterpart has only one DOF, which significantly reduces the complexity of motion control. In this article, we present a robotic gripper derived from a unit that is based on the thick panel six-crease waterbomb origami. Four such units complete the gripper. Kinematically, each unit is a plane-symmetric Bricard linkage, and the gripper can be modelled as an assembly of Bricard linkages, giving it single mobility. A gripper prototype was made using 3D printing technology, and its motion was controlled by a set of tendons tied to a single motor. Detailed kinematic modelling was done, and experiments were carried out to characterise the gripper’s behaviours. The positions of the tips on the gripper, the actuation force on tendons, and the grasping force generated on objects were analysed and measured. The experimental results matched well with the analytical ones, and the repeated tests demonstrate that the concept is viable. Furthermore, we observed that the gripper was also capable of grasping non-symmetrical objects, and such performance is discussed in detail in the paper.
The Impacts of Climate Change and Porous Pavements on Combined Sewer Overflows: A Case Study of the City of Buffalo, New York, USA
Authors: Roseboro Andrew , Torres Maria Nariné , Zhu Zhenduo , Rabideau Alan J.
Published: September 2021

Combined sewer overflows (CSOs) release pollutants collected in urban runoff into local waterways, impacting both aquatic life and human health. The impact of climate change on precipitation may result in an increase in the frequency and magnitude of heavy precipitation events, with a corresponding increase in CSO discharges. The installation of Green Infrastructure (GI) such as Porous Pavements (PP) is a resilient approach to mitigate CSO events. However, an understanding of the impact of climate change on CSO events and the effectiveness of GI practices is crucial for designing sustainable urban stormwater management systems. Using the Storm Water Management Model (SWMM), the performance of PP as a CSO abatement strategy was studied for the city of Buffalo, New York, USA. This paper used the Intensity-Duration-Frequency (IDF) curves for current (1970–1999) and future (2070–2099) design rainfall scenarios, with four rainfall durations (1, 6, 12, and 24 hours) and four return periods (2, 10, 50, and 100 years). The simulation results show that (1) current 100-year events generate CSO volumes similar to predicted 50-year events; (2) CSO volumes could increase by 11–73% in 2070–2099 compared to 1970–1999 when no GI intervention is performed; and (3) the installation of PP can reduce 2–31% of future CSO volume. This case study demonstrates the regional CSO challenges posed by climate change and supports the use of GI as a mitigation strategy.
Antilope, A Portable Low-Cost Sensor System for the Assessment of Indoor and Outdoor Air Pollution Exposure
Authors: Lenartz Fabian , Dury Marie , Bergmans Benjamin , Hutsemékers Virginie , Broun Valéry , Brose Christophe , Guichaux Sylvain
Published: September 2021

The increasing availability of low-cost sensors and open source projects make it easier than ever for a maker to build his own air quality node. Nonetheless, depending on one’s goal and its related data quality objective, to customize an existing project or to build a specific printed circuit board may still be very useful. In the framework of the Outdoor and Indoor Exposure project, a portable mini-station has been developed, tested and then used in two experiments: exposure assessment and complementary network measurement. The present paper focuses on the description of the equipment that was designed and prototyped, as well as on the tests that were made in the lab and in the field to evaluate its overall performance and that of its different sensors. Finally, we present what we consider to be its main drawbacks and our perspectives for further development and tests.
Rational Selection of Mixed Yeasts Starters for Agave Must Fermentation
Authors: Larralde-Corona Claudia Patricia , De la Torre-González Francisco Javier , Vázquez-Landaverde Pedro Alberto , Hahn Dittmar , Narváez-Zapata José Alberto
Published: September 2021

Tequila and mezcal are both traditional Mexican liquors that are produced from cooked Agave spp. must fermentation and usually rely on spontaneous or pure Saccharomyces cerevisiae strain inoculation. In order to contribute to the rational selection of yeast starters for tequila and mezcal productions, we tested a collection of 25 yeasts originally isolated from mezcal musts, spanning 10 different yeast species. These strains were first characterized in a semi synthetic medium (labeled as M2, having 90 g/L fructose and 10 g/L glucose of initial hexoses) at 48 h of culture, observing a differential pattern in the consumption of sugars and productivity. Selected Saccharomyces strains left around 10 g/L of fructose and showed higher fermentation performance. However, some non-Saccharomyces strains, specifically from Torulospora (Td), Kluyveromyces (Km), and Zygosaccharomyces (Zb) genera, consumed almost all the sugar (i.e., Km1Y9 with <5 g/L) and had a high productivity of ethanol. In general, all Saccharomyces strains presented a high production of ethyl-butyrate, ethyl-decanoate, and ethyl-hexanoate with peaks of 10, 38, and 3 μg/L, respectively. In addition, some Kluyveromyces and Torulospora strains showed a high production of phenyl ethyl acetate (i.e., Km1D5 with up to 1400 μg/L); isoamyl acetate (i.e., Km1D5 and Td1AN2 with more than 300 μg/L), and hexyl acetate (i.e., Td1AN2 with 0.3 μg/L). Representative strains of the most productive genera (Saccharomyces, Torulospora, and Kluyveromyces) were selected to evaluate their fermentative performance and survival in a mixed culture on a medium based on Agave tequilana must, and their population kinetics was characterized using specific fluorescent in situ hybridization (FISH) probes in a qualitative and semi-quantitative analysis during fermentation. We observed that the mixture ratios of 0.1:1:1 or 1:1:1 (Saccharomyces:Kluyveromyces:Torulospora), maintained good fermentation productivities, with alcohol yields above 0.45 g/g, and allowed a high survival rate of the non-Saccharomyces strains during the fermentation process. Finally, mixed inoculum fermentations on A. tequilana must medium, including different Saccharomyces strains and the finally selected Torulospora and Kluyveromyces strains, showed the best production parameters in terms of ethanol, carbon dioxide, glycerol, and acetic acid values, as well as improved volatile metabolite profiles as compared to the pure cultures. All these data were used to propose a methodology of selection of strains to be used as a pure or mixed starter for tequila and mezcal fermentations, with high primary metabolite productivity and desired aromatic profile.
Pharmacokinetic, Clinical, and Myeloid Marker Responses to Acepromazine Sedation in Arabian Camels
Authors: Kandeel Mahmoud , Almubarak Adel I. , Hussen Jamal , El-Deeb Wael , Venugopala Katharigatta N.
Published: September 2021

Sedatives and tranquilizers are important in the control of excited camels during camel transport. This study was conducted to investigate the clinical sedation of camels with acepromazine and its correlation with pharmacokinetics and pharmacodynamics. The sedation score, heart rate, respiration, body temperature, and pharmacokinetics were monitored before and after acepromazine injection, and myeloid marker expression was analyzed using membrane immunofluorescence and flow cytometry. The distribution (t1/2α) and elimination (t1/2β) half-lives were 0.1 and 9.4 h, respectively. The volume of distribution at steady state (Vss) was 20.01 L/kg, and the mean residence time (MRT) was 12.25 h. Sedation started rapidly within 10 min followed by persistent low-medium sedation for 2 h with an average sedation score of 1.2 ± 0.61, which might be associated with a slow elimination phase and prolonged MRT. Compared to horses, camels showed a lower clearance rate, higher volume of distribution, and higher elimination half-life. Slight changes in body temperature and heart and respiratory rate, as well as a lower hematocrit and changes in blood cell composition, suggest the careful application of acepromazine in animals with abnormal blood parameters or poor vital conditions.
A New Look at Vaccine Strategies Against PPRV Focused on Adenoviral Candidates
Authors: Rojas José M. , Sevilla Noemí , Martín Verónica
Published: September 2021

Peste des petits ruminants virus (PPRV) is a virus that mainly infects goats and sheep causing significant economic loss in Africa and Asia, but also posing a serious threat to Europe, as recent outbreaks in Georgia (2016) and Bulgaria (2018) have been reported. In order to carry out the eradication of PPRV, an objective set for 2030 by the Office International des Epizooties (OIE) and the Food and Agriculture Organization of the United Nations (FAO), close collaboration between governments, pharmaceutical companies, farmers and researchers, among others, is needed. Today, more than ever, as seen in the response to the SARS-CoV2 pandemic that we are currently experiencing, these goals are feasible. We summarize in this review the current vaccination approaches against PPRV in the field, discussing their advantages and shortfalls, as well as the development and generation of new vaccination strategies, focusing on the potential use of adenovirus as vaccine platform against PPRV and more broadly against other ruminant pathogens.
Development of the EIRSAT-1 CubeSat through Functional Verification of the Engineering Qualification Model
Authors: Walsh Sarah , Murphy David , Doyle Maeve , Reilly Jack , Thompson Joseph , Dunwoody Rachel , Erkal Jessica , Finneran Gabriel , Fontanesi Gianluca , Mangan Joseph , Marshall Fergal , Salmon Lána , de Faoite Daithí , Hanlon Lorraine , Martin-Carrillo Antonio , McKeown David , O’Connor William , Uliyanov Alexey , Wall Ronan , McBreen Sheila
Published: September 2021

The Educational Irish Research Satellite (EIRSAT-1) is a 2U CubeSat developed at University College Dublin. The project aims to build, test, launch, and operate Ireland’s first satellite and to perform in-orbit demonstrations of three novel payloads developed in-house. To reduce risk within the mission, the project employs a prototype model philosophy in which two models of the spacecraft exist: an engineering qualification model (EQM) and a flight model (FM). This paper presents the verification approach of the functional tests implemented for the EIRSAT-1 project. The activities of the FlatSat and system level full functional tests of the EQM are presented and the results obtained during the test campaigns are discussed. Four test anomalies were encountered during the full functional test campaign resulting in two minor redesigns, and subsequent reassembly, of the CubeSat. The functional test campaigns highlighted the importance of FlatSat level testing of CubeSats to ensure compatibility of all subsystems prior to assembly and of thorough documentation to diagnose any unexpected behaviour of the hardware efficiently. The functional verification of the EQM proved that the system conformed to its design, verifying 57 mission requirements, and is a crucial step towards the development of the EIRSAT-1 FM.
Growth Performance of Malaysian Parthenium hysterophorus under Various Environmental Variables
Authors: Ruzmi Rabiatuladawiyah , Akhir Anis Fadzilah Muhammad , Dilipkumar Masilamany , Sinniah Uma Rani , Juraimi Abdul Shukor , Ghazali Mohd Norsazwan , Ahmad-Hamdani Muhammad Saiful
Published: September 2021

Parthenium weed is an invasive weed species of economic importance worldwide. Native to the American tropics, the infestation ability of Parthenium weed to a new habitat is largely influenced by environmental factors. Despite Parthenium weed invasion in Malaysia dated back to 2013, investigation on its ecological behavior is still lacking. Hence, extensive studies on the ecological behavior of two predominant Malaysian Parthenium weed populations were executed. In the Petri dish seed bioassay, germination of Parthenium weed seeds was evident at temperatures up to 80 °C. Parthenium weed was also germinable in saline condition of up to 250 mM, osmotic pressure ranging from −1.2 to 0 MPa, and a wide range of pH (4–9), thus these abiotic conditions are by no means the limiting factors for the Parthenium weed. The pot trial observed that this invasive weed grew readily in various Malaysian soil textures. Parthenium weed successfully emerged from 0 cm to not beyond 2 cm soil burial and retained its emergence capacity under different submergence periods in water. The most favorable soil moisture condition for Parthenium weed emergence was saturated (0 kPa), followed by field capacity (−30–−50 kPa), while no emergence occurred in drought (−70 kPa) as well as flooded soils. These indicate that both Parthenium weed populations possess high tolerance to various abiotic conditions in Malaysia. Results obtained in the current study have crucially become guidelines for the local government authorities in predicting wide spread of Parthenium weed in diverse ecological zones, to further manage this pernicious weed efficiently.
Influence of Drying Methods and Vacuum Impregnation on Selected Quality Factors of Dried Sweet Potato
Authors: Kręcisz Magdalena , Kolniak-Ostek Joanna , Stępień Bogdan , Łyczko Jacek , Pasławska Marta , Musiałowska Justyna
Published: September 2021

A comparative study was conducted on the effect of vacuum impregnation and drying methods on the production of dried sweet potatoes with high bioactivity. The present study determined the quality and physicochemical properties of dried sweet potato depending on the used impregnating solutions. Sweet potatoes were pretreated in freshly squeezed onion (O) and kale (K) juice, onion and kale (OK) mixture (1:1), and sodium chloride solution. Vacuum impregnation (VI) was carried out under reduced pressure (6 kPa) for 2 min, after which the impregnating solution was dispensed and maintained under vacuum for 4 min. Sweet potato slices were dried using vacuum drying (VD) and freeze drying (FD) methods. Higher content of polyphenols, carotenoids, and chlorophyll, as well as increased antioxidant capacity and higher VOC profile, were recorded for the dried products obtained using the sublimation method. The use of vacuum impregnation reduced the hardness of the products and other textural characteristics, such as cohesiveness, springiness, and gumminess. Furthermore, we found that vacuum impregnation pretreatment was a beneficial method for improving the characteristics of dried vegetables. The highest content of phenolics, carotenoids, and chlorophylls in dried sweet potatoes were recorded when samples were impregnated with onion and kale juice. For the fresh sweet potato, the highest contribution in VOC composition had phenylacetaldehyde (44.90%), (E)-2-octen-1-al (9.43%), and benzaldehyde (8.18%), respectively. The interesting content of VOC profile compounds in dried sweet potatoes was recorded when samples were impregnated with kale juice.
Optimization of Clamping and Conveying Device for Sunflower Oil Combine Harvester Header
Authors: Liu Yang , Luo Chengming , Zong Wangyuan , Huang Xiaomao , Ma Lina , Lian Guodang
Published: September 2021

To solve the problem of grain spatter during harvest by combine harvester headers, a new clamping and conveying device of the sunflower oil harvester header was designed. We investigated plant states during the conveying process. To optimize the parameters of the sunflower oil harvester header, a test bench was built to simulate sunflower plant harvest. The influence of the clamping gap, clamping speed ratio, and clamping length on conveying success rate was explored by a single-factor experiment. Based on this experiment, the secondary regression orthogonal rotation test was carried out. The optimal structural parameter combination was obtained as follows: the clamping gap was 20 mm, the clamping speed ratio was 1.3, and the clamping length was 345 mm. Under this combination parameter condition, the corresponding conveying success rate reached up to 85.16% and the minimum value of conveying grain loss rate was 1.57%. To verify the effect of parameter optimization, a verification test and a comparison test were performed. Results showed that the actual conveying success rate was 83.50% and the actual grain loss rate was 1.49%, which were close to the optimized parameter value. The comparison test showed that the conveying success rate of the flexible clamping and conveying device was 83.50% with a grain loss rate of 1.49%, and that of the rigid clamping and conveying device was 55% with a grain loss rate of 5.17%. This study provides a theoretical basis for the design of a low-loss sunflower oil combine harvester header.
Dairy Effluent-Saturated Biochar Alters Microbial Communities and Enhances Bermudagrass Growth and Soil Fertility
Authors: Niraula Sarbjeet , Choi Yong-Keun , Payne Kristen , Muir James P. , Kan Eunsung , Chang Woo-Suk
Published: September 2021

Recently, biochar has been proposed for various agronomic applications including improved plant growth and soil fertility. In this study, the effects of dairy effluent-saturated (SBC) and unsaturated wood-derived biochar (UBC) on Bermudagrass (Cynodon spp.) growth, soil fertility and microbial communities were investigated in a greenhouse pot study. SBC and UBC were mixed with sandy loam soil at various loading rates (0, 1, 2, 4, and 8%) to grow Bermudagrass for 10 weeks. Soil physicochemical properties and plant growth measurements were taken, followed by 16S rRNA (V3-V4) amplicon sequencing of soil bacterial communities. Amendment of SBC to soil altered the soil physicochemical properties and increased the concentrations of N and P in the soil at 2 to 8% loading rates compared to UBC treated soil. The addition of SBC to soil also increased the overall plant biomass compared to UBC with more effects on aboveground biomass. Differential abundance analysis of taxa showed enrichment of Proteobacteria in UBC-amended soil, whereas Firmicutes and Nitrospirae were abundant in SBC-amended soil. Interestingly, enrichment of photosynthetic and N-fixing bacteria was observed in both SBC and UBC-amended soils after 10 weeks of treatments. However, oxidative phosphorylation and biotin metabolisms were found to be more abundant in SBC-amended soil compared to UBC-amended soil. Overall, our study suggested that amendment of SBC to soil resulted in enhanced soil nutrients, microbial capacity and Bermudagrass growth than that of UBC. Therefore, application of SBC to soil in field trials would be merited to identify sustainable and effective practices for enhancing plant growth, soil fertility and soil bacterial community.
Biochar and Bacillus sp. MN54 Assisted Phytoremediation of Diesel and Plant Growth Promotion of Maize in Hydrocarbons Contaminated Soil
Authors: Ali Muhammad Hayder , Khan Muhammad Imran , Bashir Safdar , Azam Muhammad , Naveed Muhammad , Qadri Rashad , Bashir Saqib , Mehmood Fizza , Shoukat Muhammad Aqeel , Li Yunzhou , Alkahtani Jawaher , Elshikh Mohamed S. , Dwiningsih Yheni
Published: September 2021

Contamination by petroleum hydrocarbons (PHs) is a great threat to environment due to the higher persistence and bio-toxicity of PHs. Therefore, removal of PHs from contaminated environment and strategies to reduce their toxic effects on living organisms are crucial for environmental safety and human health. The toxic effects of PHs from the polluted soil can be reduced by the addition of microbes and biochar. In this study, a pot trial was carried out to evaluate the effects of sugarcane bagasse (SB) biochar and Bacillus sp. MN54 addition on phytoremediation of PHs and growth of maize (Zea mays L.) in soil artificially contaminated with diesel. Maize seeds were sown in uncontaminated or contaminated (with PHs) soil, treated with biochar and Bacillus sp. MN54. The results revealed that PHs showed significant phytotoxicity to maize plants and the application of strain MN54 and biochar greatly reduced the toxic effects of PHs on plants growth and physiology by increasing the nutrients uptake in PHs contaminated soil. Interestingly, the phytotoxicity of PHs on maize plants was further reduced in the co-supplementation of strain MN54 and biochar. Plants physiological (25–48%) and agronomic (38–47%) attributes were significantly higher as compared to only PHs contaminated soil in the co-supplementation of strain MN54 and biochar. Similarly, nitrogen (41%), phosphorus (43%) and potassium (37%) concentrations were also increased in the co-supplementation of strain MN54 and biochar. Furthermore, maize plants successfully phytoremediate a considerable amount of PHs from soil particularly in the presence of strain MN54 and biochar, and this PHs removal was further enhanced in the co-supplementation of strain MN54 and biochar (i.e., 46% and 77% of initial PHs were removed in unplanted and planted treatments, respectively). The present results indicate that co-supplementation of biochar and Bacillus sp. MN54 could be effective in enhancing the degradation of PHs and improving plant growth in the hydrocarbons contaminated soil.