bluuebunny/crossref_metadata_2025 huggingface dataset:
New Light on Historical Specimens Reveals a New Species of Ladybird (Coleoptera: Coccinellidae): Morphological, Museomic, and Phylogenetic Analyses
Authors: Salazar Karen , Nattier Romain
Published: November 2020

Natural history collections house an important source of genetic data from yet unexplored biological diversity. Molecular data from museum specimens remain underexploited, which is mainly due to the degradation of DNA from specimens over time. However, Next-Generation Sequencing (NGS) technology can now be used to sequence “old” specimens. Indeed, many of these specimens are unique samples of nomenclatural types and can be crucial for resolving systematic or biogeographic scientific questions. Two ladybird beetle specimens from Patagonia corresponding to a new species of the genus Eriopis Mulsant were found in the collections of the Muséum national d’Histoire naturelle (MNHN), Paris. Here, we describe Eriopis patagonia Salazar, sp. nov. Total DNA of one of the two specimens was sequenced by NGS using a paired-end Illumina approach. We reconstruct and characterize the mitochondrial genome of this species (16,194 bp). Then, the protein-coding genes (PCGs) and ribosomal RNAs (rRNAs) were used to infer by maximum likelihood and Bayesian Inference the phylogenetic position of E. patagonia among 27 representatives of Coccinellidae. Phylogenetic analysis confirmed the position of Eriopis as sister group to Cycloneda Crotch. Hence, we highlight the high potential of sequencing technology for extracting molecular information from old specimens, which are used here for the systematic study of a genus, while demonstrating the importance of preserving biological collections.
Substantial Genetic Progress in the International Apis mellifera carnica Population Since the Implementation of Genetic Evaluation
Authors: Hoppe Andreas , Du Manuel , Bernstein Richard , Tiesler Friedrich-Karl , Kärcher Martin , Bienefeld Kaspar
Published: November 2020

The Apis mellifera carnica subspecies of the honeybee has long been praised for its gentleness and good honey yield before systematic breeding efforts began in the early 20th century. However, before the introduction of modern techniques of genetic evaluation (best linear unbiased prediction, BLUP) and a computerized data management in the mid 1990s, genetic progress was slow. Here, the results of the official breeding value estimation in BeeBreed.eu are analyzed to characterize breeding progress and inbreeding. From about the year 2000 onward, the genetic progression accelerated and resulted in a considerable gain in honey yield and desirable properties without increased inbreeding coefficients. The prognostic quality of breeding values is demonstrated by a retrospective analysis. The success of A. m. carnica breeding shows the potential of BLUP-based breeding values and serves as an example for a large-scale breeding program.
The Entropy Model for Sustainability Assessment in Industrial Ecosystems
Authors: Tolstykh Tatyana , Shmeleva Nadezhda , Vertakova Yulia , Plotnikov Vladimir
Published: November 2020

The aim of this paper is to address the gap in the academic literature towards the development of methodological approaches to the industrial ecosystem sustainability assessment. This study was focused on the industrial ecosystems formed based on an entropy model and implementing the principles of complex systems. This article systematizes the problem of applying the ecosystem approach to cross-industry interaction. A contribution to the literature was achieved by providing a systemic perspective on the sustainable industrial process. In this paper, we develop the methodological foundations to improve the understanding of integration processes’ influence on the industrial ecosystem potential. For a relevant analysis of industrial ecosystem potential, the existing patterns of system functioning were taken into account, including entropy equilibrium and the Harrington model. We illustrate our assumptions with an empirical case study of the National University of Science and Technology (NUST) “MISIS” ecosystem—“Green technologies for resource conservation” (Russia), with an assessment of ecosystem sustainability through the actors’ collaboration level. The propositions arising from this analysis provide information to help academics, policymakers, government, and individual enterprises with a more adequate understanding of the practical mechanisms and tools that help trigger the self-organization and sustainability of the industrial ecosystems.
Measured Rogue Waves and Their Environment
Authors: Orzech Mark D. , Wang David
Published: November 2020

We conduct a comprehensive analysis of over 350 years of accumulated ocean surface elevation time series and examine evidence for the effects of nonlinear frequency modulation, wave directional spread, surface current shear, and wind forcing on the likelihood of rogue wave development. Hourly sections of positional time series from 34 surface buoys are examined to identify over 8000 rogue wave events, recording wave sizes, times of occurrence, and geographic locations. The initial dataset is subjected to a quality control process to identify and remove false positives. We investigate the correlation of rogue events with the specified marine environmental factors in an attempt to validate the predictions of earlier theoretical and modeling analyses. The rogue event dataset is contrasted with a control, non-rogue dataset containing a total of nearly 510,000 hourly data segments of surface wave data. The analysis combines the wave records with surface current and wind data from state-of-the-art global coupled models. Statistics of the identified rogue events are summarized, and results of the environmental factor analysis are presented and discussed. This study finds some support for a causal connection between each of the environmental factors and the development of rogue waves. Results also suggest that localized environmental conditions at specific sites, such as seasonal variations in directional spread and/or high surface current vorticity, may provide useful signals of greater rogue wave threat.
Dry Port: A Review on Concept, Classification, Functionalities and Technological Processes
Authors: Varese Erica , Marigo Danilo , Lombardi Mariarosaria
Published: November 2020

The purpose of this article is to offer a literature review on the development and classification of inland terminals, later defined as “dry ports”. The aim of the paper is to analyze the extant literature on dry ports focusing on their concept, classification, function and technological processes. The review offers an updated structured approach to what is currently defined as a dry port. To this end, a structured keyword search in major electronic databases has been conducted to find related material. As there are many different names indicating dry ports in European, South East Asian and North American countries, the following keywords were used: “dry port”, “inland terminal”, “freight village” and “interporto/i”. The search was conducted in respect of the article title and text, abstract and keywords. The results show that there is no unanimous consensus concerning cataloguing of terrestrial nodal facilities serving port gateways. “Dry ports” have emerged as fundamental elements of the integration between the sea “system” and the land network. The increased interest in the genesis and development of dry ports has been accompanied by an abundant contribution of the scientific community, originating a thriving literature, which, however, does not find a common denominator.
Potassium Ferrate (VI) as the Multifunctional Agent in the Treatment of Landfill Leachate
Authors: Thomas Maciej , Kozik Violetta , Barbusiński Krzysztof , Sochanik Aleksander , Jampilek Josef , Bąk Andrzej
Published: November 2020

Possible use of potassium ferrate (VI) (K2FeO4) for the treatment of landfill leachate (pH = 8.9, Chemical Oxygen Demand (COD) 770 mg O2/L, Total Organic Carbon (TOC) 230 mg/L, Total Nitrogen (Total N) 120 mg/L, Total Phosphorus (Total P) 12 mg/L, Total Coli Count (TCC) 6.8 log CFU/mL (Colony-Forming Unit/mL), Most Probable Number (MPN) of fecal enterococci 4.0 log/100 mL, Total Proteolytic Count (TPC) 4.4 log CFU/mL) to remove COD was investigated. Central Composite Design (CCD) and Response Surface Methodology (RSM) were applied for modelling and optimizing the purification process. Conformity of experimental and predicted data (R2 = 0.8477, Radj2 = 0.7462) were verified using Analysis of Variance (ANOVA). Application of K2FeO4 using CCD/RSM allowed to decrease COD, TOC, Total N, Total P, TCC, MPN of fecal enterococci and TPC by 76.2%, 82.6%, 68.3%, 91.6%, 99.0%, 95.8% and 99.3%, respectively, by using K2FeO4 0.390 g/L, at pH = 2.3 within 25 min. Application of equivalent amount of iron (as FeSO4 × 7H2O and FeCl3 × 6H2O) under the same conditions allowed to diminish COD, TOC, Total N, Total P, TCC, MPN of fecal enterococci and TPC only by 38.1%, 37.0%, 20.8%, 95.8%, 94.4%, 58.2%, 90.8% and 41.6%, 45.7%, 29.2%, 95.8%, 92.1%, 58.2%, 90.0%, respectively. Thus, K2FeO4 could be applied as an environmentally friendly reagent for landfill leachate treatment.
Mechanical Properties of High-Strength Cu–Cr–Zr Alloy Fabricated by Selective Laser Melting
Authors: Sun Fujia , Liu Ping , Chen Xiaohong , Zhou Honglei , Guan Pengfei , Zhu Bilan
Published: November 2020

The approximate process range for preparing the Cu–Cr–Zr alloy by selective laser melting (SLM) was determined by ANSYS simulation, and the influence of the SLM process parameters on the comprehensive properties of the SLM-formed alloy was studied by the design of experiments. The Cu–Cr–Zr alloy with optimum strength and hardness was prepared with high efficiency by optimizing the process parameters for SLM (i.e., laser power, scanning speed, and hatching distance). It is experimentally shown that tensile strength and hardness of the SLM alloy are increased by increasing laser power and decreasing scanning speed, whereas they are initially increased and then decreased by increasing the hatching distance. Moreover, strength, roughness and hardness of the SLM alloy are optimized when laser power is 460 W, scanning speed is 700 mm/s and hatching distance is 0.06 mm. The optimized properties of the SLM alloy are a tensile strength of 153.5 MPa, hardness of 119 HV, roughness of 31.384 μm and relative density of 91.62%.
Iron Oxide/Phosphatic Materials Composites with Potential Applications in Environmental Protection
Authors: Ispas Georgiana Cornelia , Manea Raluca , Brazdis Roxana Ioana , Baroi Anda Maria , Fistos Toma , Fierascu Radu Claudiu , Raduly Monica Florentina
Published: November 2020

Currently, hydroxyapatite is probably the most researched material, due to its multiple applications in medical, environmental, or cultural heritage, when the classical structure is modified and calcium is displaced partially or totally with different metals. By changing the classical structure of the hydroxyapatite, new morphologies can be obtained, thus allowing final applications different from those of the initial hydroxyapatite material. However, their properties should be tuned for the desired application. In this context, the present paper describes the synthesis and characterization (through energy-dispersive X-ray fluorescence, X-ray diffraction, FTIR, thermal analysis, and transmission electron microscopy) of iron oxide/manganese-containing phosphatic phase composite materials, developed in order to obtain the enhancement of final environmental applications (photodegradation of dyes, adsorption of organic compounds). The composite material was tested for photocatalytic properties, after embedding in hydrosoluble film-forming materials. Photocatalytic coatings show different activity during the photodecomposition of Methylene Blue, used as a model of a contaminant. The photocatalytic activities of the materials were discussed in relationship with both the phosphatic materials and the magnetic components. Finally, other environmental applications were studied for the developed materials (adsorption of non-steroidal anti-inflammatory drugs—paracetamol and ibuprofen), revealing an enhancement of the adsorption capacity of the phosphatic material upon addition of the magnetic phase.
Novel Caryophyllane-Related Sesquiterpenoids with Anti-Inflammatory Activity from Rumphella antipathes (Linnaeus, 1758)
Authors: Chang Yu-Chia , Chiang Chih-Chao , Chang Yuan-Shiun , Chen Jih-Jung , Wang Wei-Hsien , Fang Lee-Shing , Chung Hsu-Ming , Hwang Tsong-Long , Sung Ping-Jyun
Published: November 2020

Two previously undescribed caryophyllane-related sesquiterpenoids, antipacids A (1) and B (2), with a novel bicyclo[5.2.0] core skeleton, and known compound clovane-2β,9α-diol (3), along with rumphellolide L (4), an esterified product of 1 and 3, were isolated from the organic extract of octocoral Rumphella antipathes. Their structures, including the absolute configurations were elucidated by spectroscopic and chemical experiments. In vivo anti-inflammatory activity analysis indicated that antipacid B (2) inhibited the generation of superoxide anions and the release of elastase by human neutrophils, with IC50 values of 11.22 and 23.53 μM, respectively, while rumphellolide L (4) suppressed the release of elastase with an IC50 value of 7.63 μM.
Spirulina maxima Derived Pectin Nanoparticles Enhance the Immunomodulation, Stress Tolerance, and Wound Healing in Zebrafish
Authors: Rajapaksha Dinusha C. , Edirisinghe Shan L. , Nikapitiya Chamilani , Dananjaya SHS , Kwun Hyo-Jung , Kim Cheol-Hee , Oh Chulhong , Kang Do-Hyung , De Zoysa Mahanama
Published: November 2020

In this study, Spirulina maxima derived pectin nanoparticles (SmPNPs) were synthesized and multiple biological effects were investigated using in vitro and in vivo models. SmPNPs were not toxic to Raw 264.7 cells and zebrafish embryos up to 1 mg/mL and 200 µg/mL, respectively. SmPNPs upregulated Il 10, Cat, Sod 2, Def 1, Def 2, and Muc 1 in Raw 264.7 cells and tlr2, tlr4b, tlr5b, il1β, tnfα, cxcl8a, cxcl18b, ccl34a.4, ccl34b.4, muc5.1, muc5.2, muc5.3, hamp, cstd, hsp70, cat, and sod1 in the larvae and adult zebrafish, suggesting immunomodulatory activity. Exposure of larvae to SmPNPs followed by challenge with pathogenic bacterium Aeromonas hydrophila resulted a two-fold reduction of reactive oxygen species, indicating reduced oxidative stress compared to that in the control group. The cumulative percent survival of larvae exposed to SmPNPs (50 µg/mL) and adults fed diet supplemented with SmPNPs (4%) was 53.3% and 76.7%, respectively. Topical application of SmPNPs on adult zebrafish showed a higher wound healing percentage (48.9%) compared to that in the vehicle treated group (38.8%). Upregulated wound healing markers (tgfβ1, timp2b, mmp9, tnfα, il1β,ccl34a.4, and ccl34b.4), enhanced wound closure, and restored pigmentation indicated wound healing properties of SmPNPs. Overall, results uncover the multiple bioactivities of SmPNPs, which could be a promising biocompatible candidate for broad range of aquatic and human therapies.
Development of Al-TiC Wire Feedstock for Additive Manufacturing by Metal Screw Extrusion
Authors: Langelandsvik Geir , Grandcolas Mathieu , Skorpen Kristian G. , Furu Trond , Akselsen Odd M. , Roven Hans Jørgen
Published: November 2020

The development of customised aluminium alloys for welding and additive manufacturing (AM) is proposed to solve several quality issues and to enhance the mechanical integrity of components. The introduction of ceramic grain refining agents shows great potential as alloy addition as to limit cracking susceptibility and increase the strength. Thus, a versatile solid-state manufacturing route for nanoparticle reinforced aluminium wires has been developed based on the metal screw extrusion principle. In fact, the Al-Si alloy AA4043 mixed with 1 wt.% TiC nanoparticles has been manufactured as a wire. The accumulated strain on the material during metal screw extrusion has been estimated, classifying the process as a severe plastic deformation (SPD) method. A chemical reaction between silicon and TiC particles after metal screw extrusion was found, possibly limiting the grain refining effect. Electric arc bead-on-plate deposition was performed with metal screw extruded and commercial material. The addition of TiC induced a grain morphology transition from columnar to equiaxed after electric arc deposition, and increased the hardness. A high amount of porosity was found in the AA4043-TiC material, probably arising from hydrogen contamination on TiC surfaces prior to metal screw extrusion. The results are encouraging as a new direction for aluminium alloy development for additive manufacturing.
Antioxidant Properties of Lyophilized Rosemary and Sage Extracts and its Effect to Prevent Lipid Oxidation in Poultry Pátê
Authors: Bianchin Mirelli , Pereira Daiane , Almeida Jacqueline de Florio , Moura Cristiane de , Pinheiro Rafaelly Simionatto , Heldt Leila Fernanda Serafini , Haminiuk Charles Windson Isidoro , Carpes Solange Teresinha
Published: November 2020

This study aimed to evaluate the antioxidant activities (AA) of lyophilized rosemary extract and lyophilized sage extract, and their effects on the oxidative stability of poultry pátê. For this purpose, four poultry pátê formulations with rosemary, sage, sodium erythorbate and a control (without antioxidants) were produced. The rosemary and sage were characterized according to total phenolic compounds (TPC) and AA by several methods. The poultry pátês stored at 4 °C were evaluated by the lipid oxidation. High concentrations of TPC were detected in the rosemary extract and sage extract (46.48 and 41.61 mg GAE/g (Gallic acid equivalent), respectively). The AA of the rosemary and sage extracts determined by free radical-scavenging were 4745.72 and 2462.82 µmol TE/g (Trolox equivalents), respectively. The high concentrations of catechin, rutin, myricetin and p-coumaric acids in these extracts may be responsible for the strong inhibitory action against food pathogens. Besides this, these compounds can be responsible for the best performance in inhibiting lipid oxidation in poultry pátês during storage. This study suggests that rosemary and sage extracts may be used as a natural antioxidant in meat products.
Cyanobacteria and Microalgae as Sources of Functional Foods to Improve Human General and Oral Health
Authors: Ferrazzano Gianmaria Fabrizio , Papa Cristina , Pollio Antonino , Ingenito Aniello , Sangianantoni Giancarla , Cantile Tiziana
Published: November 2020

In the scenario of promising sources of functional foods and preventive drugs, microalgae and cyanobacteria are attracting global attention. In this review, the current and future role of microalgae as natural sources of functional foods for human health and, in particular, for oral health has been reported and discussed in order to provide an overview on the state of art on microalgal effects on human oral health. It is well known that due to their richness in high-valuable products, microalgae offer good anti-inflammatory, antioxidant, antitumoral, anti-glycemic, cholesterol-lowering, and antimicrobial activity. Moreover, the findings of the present research show that microalgae could also have a significant impact on oral health: several studies agree on the potential application of microalgae for oral cancer prevention as well as for the treatment of chronic periodontitis and different oral diseases with microbial origin. Thus, beneficial effects of microalgae could be implemented in different medical fields. Microalgae and cyanobacteria could represent a potential natural alternative to antibiotic, antiviral, or antimycotic therapies, as well as a good supplement for the prevention and co-adjuvant treatment of different oral diseases. Nevertheless, more studies are required to identify strains of interest, increase overall functioning, and make safe, effective products available for the whole population.
Chemical Constituent of β-Glucuronidase Inhibitors from the Root of Neolitsea acuminatissima
Authors: Lin Chu-Hung , Chou Hsiao-Jung , Chang Chih-Chi , Chen Ih-Sheng , Chang Hsun-Shuo , Cheng Tian-Lu , Kuo Yueh-Hsiung , Ko Horng-Huey
Published: November 2020

Neolitsea acuminatissima (Lauraceae) is an endemic plant in Taiwan. One new carboline alkaloid, demethoxydaibucarboline A (1), two new eudesmanolide-type sesquiterpenes, methyl-neolitacumone A (2), neolitacumone E (3), and twelve known compounds (4–15) were isolated from the root of Neolitsea acuminatissima. Their structures were elucidated by spectroscopic analysis. Glucuronidation represents a major metabolism process of detoxification for carcinogens in the liver. However, intestinal bacterial β-Glucuronidase (βG) has been considered pivotal to colorectal carcinogenesis. To develop specific bacterial-βG inhibitors with no effect on human βG, methanolic extract of roots of N. acuminatissima was selected to evaluate their anti-βG activity. Among the isolates, demethoxydaibucarboline A (1) and quercetin (8) showed a strong bacterial βG inhibitory effect with an inhibition ratio of about 80%. Methylneolitacumone A (2) and epicatechin (10) exhibited a moderate or weak inhibitory effect and the enzyme activity was less than 45% and 74%, respectively. These four compounds specifically inhibit bacterial βG but not human βG. Thus, they are expected to be used for the purpose of reducing chemotherapy-induced diarrhea (CID). The results suggest that the constituents of N. acuminatissima have the potential to be used as CID relief candidates. However, further investigation is required to determine their mechanisms of action.
Saponins as Modulators of the Blood Coagulation System and Perspectives Regarding Their Use in the Prevention of Venous Thromboembolic Incidents
Authors: Olas Beata , Urbańska Karina , Bryś Magdalena
Published: November 2020

Saponins comprise a heterogenous group of chemical compounds containing a triterpene or steroid aglycone group and at least one sugar chain. They exist as secondary metabolites, occurring frequently in dicotyledonous plants and lower marine animals. Plant saponin extracts or single saponins have indicated antiplatelet and anticoagulant activity. Venous thromboembolism (VTE), including deep venous thrombosis and pulmonary embolism, is a multifactorial disease influenced by various patient characteristics such as age, immobility, previous thromboembolism and inherited thrombophilia. This mini-review (1) evaluates the current literature on saponins as modulators of the coagulation system, (2) discusses the impact of chemical structure on the modulation of the coagulation system, which may further provide a basis for drug or supplement design, (3) examines perspectives of their use in the prevention of VTE. It also describes the molecular mechanisms of action of the saponins involved in the prevention of VTE.
A Review of Density Functional Models for the Description of Fe(II) Spin-Crossover Complexes
Authors: Römer Anton , Hasecke Lukas , Blöchl Peter , Mata Ricardo A.
Published: November 2020

Spin-crossover (SCO) materials have for more than 30 years stood out for their vast application potential in memory, sensing and display devices. To reach magnetic multistability conditions, the high-spin (HS) and low-spin (LS) states have to be carefully balanced by ligand field stabilization and spin-pairing energies. Both effects could be effectively modelled by electronic structure theory, if the description would be accurate enough to describe these concurrent influences to within a few kJ/mol. Such a milestone would allow for the in silico-driven development of SCO complexes. However, so far, the ab initio simulation of such systems has been dominated by general gradient approximation density functional calculations. The latter can only provide the right answer for the wrong reasons, given that the LS states are grossly over-stabilized. In this contribution, we explore different venues for the parameterization of hybrid functionals. A fitting set is provided on the basis of explicitly correlated coupled cluster calculations, with single- and multi-dimensional fitting approaches being tested to selected classes of hybrid functionals (hybrid, range-separated, and local hybrid). Promising agreement to benchmark data is found for a rescaled PBE0 hybrid functional and a local version thereof, with a discussion of different atomic exchange factors.
Secondary S100B Protein Increase Following Brain Arteriovenous Malformation Rupture is Associated with Cerebral Infarction
Authors: Garzelli Lorenzo , Jacquens Alice , Amouyal Caroline , Premat Kevin , Sourour Nader , Cortese Jonathan , Haffaf Idriss , Mathon Bertrand , Lenck Stéphanie , Clarençon Frédéric , Degos Vincent , Shotar Eimad
Published: November 2020

Early S100B protein serum elevation is associated with poor prognosis in patients with ruptured brain arteriovenous malformations (BAVM). The purpose of this study is to determine whether a secondary elevation of S100B is associated with early complications or poor outcome in this population. This is a retrospective study of patients admitted for BAVM rupture. A secondary increase of S100B was defined as an absolute increase by 0.1 μg/L within 30 days of admission. Fisher’s and unpaired t tests followed by multivariate analysis were performed to identify markers associated with this increase. Two hundred and twenty-one ruptures met inclusion criteria. Secondary S100B protein serum elevation was found in 17.1% of ruptures and was associated with secondary infarction (p < 0.001), vasospasm-related infarction (p < 0.001), intensive care (p = 0.009), and hospital length of stay (p = 0.005), but not with early rebleeding (p = 0.07) or in-hospital mortality (p = 0.99). Secondary infarction was the only independent predictor of secondary increase of S100B (OR 9.9; 95% CI (3–35); p < 0.001). Secondary elevation of S100B protein serum levels is associated with secondary infarction in ruptured brain arteriovenous malformations.
Tetrapodal Anion Transporters
Authors: Gilchrist Alexander M. , Chen Lijun , Wu Xin , Lewis William , Howe Ethan N.W. , Macreadie Lauren K. , Gale Philip A.
Published: November 2020

Synthetic anion transporters that facilitate chloride transport are promising candidates for channelopathy treatments. However, most anion transporters exhibit an undesired side effect of facilitating proton transport via interacting with fatty acids present in the membrane. To address the limitation, we here report the use of a new tetrapodal scaffold to maximize the selective interaction with spherical chloride over binding the carboxylate headgroup of fatty acids. One of the new transporters demonstrated a high selectivity for chloride uniport over fatty acid-induced proton transport while being >10 times more active in chloride uniport than strapped calixpyrroles that were previously the only class of compounds known to possess similar selectivity properties.
Antiglioma Potential of Coumarins Combined with Sorafenib
Authors: Sumorek-Wiadro Joanna , Zając Adrian , Langner Ewa , Skalicka-Woźniak Krystyna , Maciejczyk Aleksandra , Rzeski Wojciech , Jakubowicz-Gil Joanna
Published: November 2020

Coumarins, which occur naturally in the plant kingdom, are diverse class of secondary metabolites. With their antiproliferative, chemopreventive and antiangiogenetic properties, they can be used in the treatment of cancer. Their therapeutic potential depends on the type and location of the attachment of substituents to the ring. Therefore, the aim of our study was to investigate the effect of simple coumarins (osthole, umbelliferone, esculin, and 4-hydroxycoumarin) combined with sorafenib (specific inhibitor of Raf (Rapidly Accelerated Fibrosarcoma) kinase) in programmed death induction in human glioblastoma multiforme (T98G) and anaplastic astrocytoma (MOGGCCM) cells lines. Osthole and umbelliferone were isolated from fruits: Mutellina purpurea L. and Heracleum leskowii L., respectively, while esculin and 4-hydroxycoumarin were purchased from Sigma Aldrich (St. Louis, MO, USA). Apoptosis, autophagy and necrosis were identified microscopically after straining with specific fluorochromes. The level of caspase 3, Beclin 1, PI3K (Phosphoinositide 3-kinase), and Raf kinases were estimated by immunoblotting. Transfection with specific siRNA (small interfering RNA) was used to block Bcl-2 (B-cell lymphoma 2), Raf, and PI3K expression. Cell migration was tested with the wound healing assay. The present study has shown that all the coumarins eliminated the MOGGCCM and T98G tumor cells mainly via apoptosis and, to a lesser extent, via autophagy. Osthole, which has an isoprenyl moiety, was shown to be the most effective compound. Sorafenib did not change the proapoptotic activity of this coumarin; however, it reduced the level of autophagy. At the molecular level, the induction of apoptosis was associated with a decrease in the expression of PI3K and Raf kinases, whereas an increase in the level of Beclin 1 was observed in the case of autophagy. Inhibition of the expression of this protein by specific siRNA eliminated autophagy. Moreover, the blocking of the expression of Bcl-2 and PI3K significantly increased the level of apoptosis. Osthole and sorafenib successfully inhibited the migration of the MOGGCCM and T98G cells.
Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation
Authors: García Daniela Armijo , Mendoza Lupe , Vizuete Karla , Debut Alexis , Arias Marbel Torres , Gavilanes Alex , Terencio Thibault , Ávila Edward , Jeffryes Clayton , Dahoumane Si Amar
Published: November 2020

Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications. To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively. The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs. The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD). The optical properties of Ag2Se were studied using UV–Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS). The biological activity of the Ag2Se NPs was assessed using cytotoxic and bactericidal approaches. Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
Daylight Photodynamic Therapy: An Update
Authors: Lee Chaw-Ning , Hsu Rosie , Chen Hsuan , Wong Tak-Wah
Published: November 2020

Daylight photodynamic therapy (dPDT) uses sunlight as a light source to treat superficial skin cancer. Using sunlight as a therapeutic device has been present for centuries, forming the basis of photodynamic therapy in the 20th century. Compared to conventional PDT, dPDT can be a less painful, more convenient and an effective alternative. The first clinical uses of dPDT on skin cancers began in Copenhagen in 2008. Currently, aminolevulinic acid-mediated dPDT has been approved to treat actinic keratosis patients in Europe. In this review article, we introduce the history and mechanism of dPDT and focus on the pros and cons of dPDT in treating superficial skin cancers. The future applications of dPDT on other skin diseases are expected to expand as conventional PDT evolves.
Evaluation of Antioxidant and Anti-α-glucosidase Activities of Various Solvent Extracts and Major Bioactive Components from the Seeds of Myristica fragrans
Authors: Li Cai-Wei , Chu Yi-Cheng , Huang Chun-Yi , Fu Shu-Ling , Chen Jih-Jung
Published: November 2020

Myristica fragrans is a well-known species for flavoring many food products and for formulation of perfume and medicated balm. It is also used to treat indigestion, stomach ulcers, liver disorders, and, as emmenagogue, diaphoretic, diuretic, nervine, and aphrodisiac. We examined antioxidant properties and bioactive compounds in various solvent extracts from the seeds of M. fragrans. Methanol, ethanol, and acetone extracts exhibited relatively strong antioxidant activities by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide radical, and hydroxyl radical scavenging tests. Furthermore, methanol extracts also displayed significant anti-α-glucosidase activity. Examined and compared to the various solvent extracts for their chemical compositions using HPLC analysis, we isolated the ten higher content compounds and analyzed antioxidant and anti-α-glucosidase activities. Among the isolates, dehydrodiisoeugenol, malabaricone B and malabaricone C were main antioxidant components in seeds of M. fragrans. Malabaricone C exhibited stronger antioxidant capacities than others based on lower half inhibitory concentration (IC50) values in DPPH and ABTS radical scavenging assays, and it also showed significant inhibition of α-glucosidase. These results shown that methanol was found to be the most efficient solvent for extracting the active components from the seeds of M. fragrans, and this material is a potential good source of natural antioxidant and α-glucosidase inhibitor.
Silicon Nanowires for Gas Sensing: A Review
Authors: Akbari-Saatlu Mehdi , Procek Marcin , Mattsson Claes , Thungström Göran , Nilsson Hans-Erik , Xiong Wenjuan , Xu Buqing , Li You , Radamson Henry H.
Published: November 2020

The unique electronic properties of semiconductor nanowires, in particular silicon nanowires (SiNWs), are attractive for the label-free, real-time, and sensitive detection of various gases. Therefore, over the past two decades, extensive efforts have been made to study the gas sensing function of NWs. This review article presents the recent developments related to the applications of SiNWs for gas sensing. The content begins with the two basic synthesis approaches (top-down and bottom-up) whereby the advantages and disadvantages of each approach have been discussed. Afterwards, the basic sensing mechanism of SiNWs for both resistor and field effect transistor designs have been briefly described whereby the sensitivity and selectivity to gases after different functionalization methods have been further presented. In the final words, the challenges and future opportunities of SiNWs for gas sensing have been discussed.
Facile Strategy for Mass Production of Pt Catalysts for Polymer Electrolyte Membrane Fuel Cells Using Low-Energy Electron Beam
Authors: Shin Jongmin , Min Jiho , Kim Youngjin , Lee Jin Hee , Chai Geunseok , Jung Namgee
Published: November 2020

There are so many variables affecting the large-scale chemical synthesis of nanoparticles that mass production remains a challenge. Here, using a high-efficiency compact electron beam generator irradiating a low-energy electron beam, we fabricate carbon-supported Pt nanoparticles (Pt/C) in an open chamber to present the applicability of an electron beam to the mass production of metal nanocatalysts for polymer electrolyte membrane fuel cells (PEMFCs). The amount of dispersants (glycerol) and radical scavengers (isopropyl alcohol, IPA), the most important factors in the electron beam-induced fabrication process, is systematically controlled to find the conditions for the synthesis of the particle structure suitable for PEMFC applications. Furthermore, the effects of the structural changes on the electrochemical properties of the catalysts are thoroughly investigated. Through in-depth studies, it is clearly revealed that while dispersants control the nucleation step of monomers affecting the degree of dispersion of nanoparticles, radical scavengers with strong oxidizing power have an effect on the particle growth rate. Therefore, this study is expected to present the applicability of low-energy electron beam to the mass production of metal nanocatalysts for PEMFCs, and to provide insights into the fabrication of nanoparticles using low-energy electron beams.
Erratum: Wang, D., et al. Self-Assembly Synthesis of the MoS2/PtCo Alloy Counter Electrodes for High-Efficiency and Stable Low-Cost Dye-Sensitized Solar Cells. Nanomaterials 2020, 10, 1725
Authors: Zeng Zhi , Wang Dongbo , Wang Jinzhong , Jiao Shujie , Huang Yuewu , Zhao Sixiang , Zhang Bingke , Ma Mengyu , Gao Shiyong , Feng Xingguo , Zhao Liancheng
Published: November 2020

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Velocity Determinants in Spastic Patients after Stroke—A Gait Analysis Study
Authors: Silva Miguel Reis e , Jacinto Jorge
Published: November 2020

Introduction: Gait velocity in spastic patients after stroke is both a life quality and mortality predictor. However, the precise biomechanical events that impair a faster velocity in this population are not defined. This study goal is to find out which are the gait parameters associated with a higher velocity in stroke patients with spastic paresis. Methods: The registries of a Gait analysis laboratory were retrospectively analyzed. The inclusion criteria were: trials of adult stroke patients with unilateral deficits. The exclusion criteria were: trials when patients used an external walking device, an orthosis, or support by a third person. Of the 116 initial patients, after the application of the exclusion criteria, 34 patients were included in the cohort, all with spatiotemporal, static and dynamic kinematic and dynamometric studies. Results: There was a correlation of velocity with cadence, stride length of the paretic (P) limb, stride length, and time of the P and non-paretic (NP) limb, double support time, all the parameters related to hip extension during stance phase, knee flexion during swing phase, and parameters related to ankle plantarflexion during stance phase. Conclusions: The main gait analysis outcomes that have a correlation with speed are related to the formula velocity = step length × cadence or are related to stance phase events that allow the anterior projection of the body. The only swing phase outcome that has a correlation with speed is knee flexion. More studies are needed from gait analysis laboratories in order to point out the most relevant goals to achieve with gait training in spastic stroke patients.
Relationship between Dispositional Mindfulness, Psychological Health, and Diet Quality among Healthy Midlife Adults
Authors: Donofry Shannon D. , Erickson Kirk I. , Levine Michele D. , Gianaros Peter J. , Muldoon Matthew F. , Manuck Stephen B.
Published: November 2020

Mindfulness, a practice of non-judgmental awareness of present experience, has been associated with reduced eating psychopathology and emotion-driven eating. However, it remains unclear whether mindfulness relates to diet quality. Thus, the purpose of this study was to examine whether dispositional mindfulness is associated with diet quality and to explore psychological factors relating dispositional mindfulness to diet quality. Community-dwelling adults (N = 406; Mage = 43.19, SD = 7.26; Mbody mass index [BMI] = 27.08, SD = 5.28; 52% female) completed ratings of dispositional mindfulness, depressive symptoms, perceived stress, positive affect (PA), and negative affect (NA). Dietary intake was assessed using the Block Food Frequency Questionnaire, from which the 2015 Healthy Eating Index was derived. Analyses were conducted using the “lavaan” package in R with bias-corrected bootstrapped confidence intervals (BootCI). Age, sex, race, education, and BMI were entered as covariates in all models. Higher dispositional mindfulness was associated with higher diet quality (β = 0.11, p = 0.03), and this effect was mediated through lower depressive symptoms (indirect effect β = 0.06, p = 0.02, BootCI = 0.104–1.42, p = 0.03). Dispositional mindfulness was negatively correlated with perceived stress (β = −0.31, p < 0.01) and NA (β = −0.43, p < 0.01), as well as positively correlated with PA (β = −0.26, p < 0.01). However, these factors were unrelated to diet quality. These cross-sectional data provide initial evidence that dispositional mindfulness relates to diet quality among midlife adults, an effect that may be explained in part by less depressive symptomatology. Given that lifestyle behaviors in midlife are leading determinants of risk for cardiovascular disease and neurocognitive impairment in late life, interventions to enhance mindfulness in midlife may mitigate disease risk. Additional research assessing the impact of mindfulness interventions on diet quality are warranted.
Animal Protein Intake Is Positively Associated with Metabolic Syndrome Risk Factors in Middle-Aged Korean Men
Authors: Chung Sangwon , Chung Min-Yu , Choi Hyo-Kyoung , Park Jae Ho , Hwang Jin-Taek , Joung Hyojee
Published: November 2020

Few studies have examined the relationship of protein intake by food source with metabolic syndrome in Korean adults, even though animal food intake has increased. This study examined the association between plant and animal protein intake and metabolic syndrome among middle-aged Korean adults. A total of 13,485 subjects aged 30–64 years were selected from the 2013–2018 Korea National Health and Nutrition Examination Survey. Protein intake was assessed using 24-h dietary recall data and divided into quintiles. Men had a higher percentage of energy intake from animal protein (7.4%) than plant protein (6.9%). Men in the highest quintile group of animal protein intake had a higher prevalence of abdominal obesity (OR: 1.30, 95% CI: 1.00–1.70), reduced high-density lipoprotein cholesterol (HDL-C) (OR: 1.43, 95% CI: 1.07–1.90), and elevated fasting glucose (OR: 1.32, 95% CI: 1.01–1.74), after adjusting for covariates. Furthermore, stronger associations of animal protein intake with abdominal obesity were shown in men who consumed less than estimated energy requirements (OR: 1.60, 95% CI: 1.11–2.31). Plant protein intake was negatively associated with increased blood pressure in men. Neither animal nor plant protein intakes were significantly associated with any of the metabolic syndrome risk factors in women. The results imply that lower animal protein intake may be a beneficial factor for metabolic syndrome management in middle-aged Korean men.
Vitamin D Metabolite Profile in Cholecalciferol- or Calcitriol-Supplemented Healthy and Mammary Gland Tumor-Bearing Mice
Authors: Anisiewicz Artur , Kowalski Konrad , Banach Joanna , Łabędź Natalia , Stachowicz-Suhs Martyna , Piotrowska Aleksandra , Milczarek Magdalena , Kłopotowska Dagmara , Dzięgiel Piotr , Wietrzyk Joanna
Published: November 2020

To analyze if the prometastatic activity of calcitriol (active vitamin D3 metabolite), which was previously observed in a 4T1 breast cancer model, is also found in other breast cancers, and to assess the impact of various schemes of vitamin D supply, we used 4T1 and E0771 mouse metastatic and 67NR nonmetastatic cells in this study. BALB/c and C57BL/6 healthy and tumor-bearing mice were exposed to a control (1000 IU), low- (100 IU), and high- (5000 IU) vitamin D3 diets. Additionally, from day 7 of tumor transplantation, the 1000 and 100 IU groups were gavaged with calcitriol (+cal). After 8 weeks of feeding, plasma levels of 25(OH)D3, 24,25(OH)2D3, and 3-epi-25(OH)D3 were significantly lower in calcitriol-treated and vitamin D-deficient groups than in the control, whereas the levels of all metabolites were increased in the 5000 IU group. The ratio of 25(OH)D3:24,25(OH)2D3 was increased in both calcitriol-treated groups, whereas the ratio of 25(OH)D3:3-epi-25(OH)D3 was increased only in the 100 IU group but decreased in the 5000 IU group. In contrast to E0771, 4T1 lung metastasis was accelerated in all vitamin D-supplemented mice, as well as in the deficient group with an increased inflammatory response. 67NR tumor growth was transiently inhibited in the 1000 IU+cal group, but single metastases were observed in the 5000 and 100 IU groups. Based on the results, we conclude that various schemes of vitamin D supply and vitamin D deficiency led to similar metabolite profiles irrespective of the mice strain and tumor burden. However, depending on the type of breast cancer, different effects on tumor growth and metastasis were noticed.
Dynamic Adjustments of Parenteral Support in Early Adult Intestinal Failure—Essential Role of Sodium
Authors: Jacob Torid , Glass Aenne , Witte Maria , Reiner Johannes , Lamprecht Georg
Published: November 2020

Intestinal failure (IF) requires parenteral support (PS) substituting energy, water, and electrolytes to compensate intestinal losses and replenish deficits. Convalescence, adaptation, and reconstructive surgery facilitate PS reduction. We analyzed the effect of changes of PS on body mass index (BMI) in early adult IF. Energy, volume, and sodium content of PS and BMI were collected at the initial contact (FIRST), the time of maximal PS and BMI (MAX) and the last contact (LAST). Patients were categorized based on functional anatomy: small bowel enterostomy—group 1, jejuno-colic anastomosis—group 2. Analysis of variance was used to test the relative impact of changes in energy, volume, or sodium. Total of 50 patients were followed for 596 days. Although energy, volume, and sodium support were already high at FIRST, we increased PS to MAX, which was accompanied by a significant BMI increase. Thereafter PS could be reduced significantly, leading to a small BMI decrease in group 1, but not in group 2. Increased sodium support had a stronger impact on BMI than energy or volume. Total of 13 patients were weaned. Dynamic PS adjustments are required in the early phase of adult IF. Vigorous sodium support acts as an independent factor.
The Role of Early Programming and Early Nutrition on the Development and Progression of Celiac Disease: A Review
Authors: Martín-Masot Rafael , Diaz-Castro Javier , Moreno-Fernandez Jorge , Navas-López Víctor Manuel , Nestares Teresa
Published: November 2020

Experimental and epidemiological evidence has shown that modifications of the intrauterine environment can have deleterious consequences for individuals, expressed as an increased risk of suffering non-communicable pathologies in adult life, which is known as the hypothesis of the early origin of diseases or fetal programming. On the other hand, changes in gene expression patterns through epigenetic modifications can be the basis for long-term maintenance of the effects of fetal programming. In this sense, epigenetics comprises the study of intrauterine disturbances, which develop diseases in the adult, including celiac disease (CD). In addition, early feeding practices could influence the risk of CD development, such as breastfeeding timing and duration and age of gluten introduction in the diet. Gluten acts as a trigger for CD in genetically predisposed subjects, although approximately 30% of the world population has HLA DQ2 or DQ8, the prevalence of the disease is only 1–3%. It is not known what factors act to modify the risk of disease in genetically at-risk subjects. Taking into account all these considerations, the aim of the current review is to elucidate the role of early programming and the effect of early nutrition on the development and progression of CD. It is logical that attention has been paid to gluten as a key element in preventing the disease. However, there is no strong evidence in favor of the protective factor of breastfeeding, timing of introduction of gluten during lactation, and the development of CD. Diet, genetic risk, microbiota, and environmental interaction are possible triggers of the change in tolerance to an immune response to gluten, but large-scale cohort studies are needed. Emerging scientific concepts, such as epigenetics, may help us establish the role of these factors.