bluuebunny/crossref_metadata_2025 huggingface dataset:
Catastrophic Antiphospholipid Syndrome
Authors: Bitsadze Victoria , Yakubova Fidan , Khizroeva Jamilya , Lazarchuk Arina , Salnikova Polina , Vorobev Alexander , Tretyakova Maria , Degtyareva Natalia , Grigoreva Kristina , Gashimova Nilufar , Kvaratskheliia Margaret , Makatsariya Nataliya , Kudryavtseva Ekaterina , Tomlenova Anna , Gris Jean-Christophe , Elalamy Ismail , Ay Cihan , Makatsariya Alexander
Published: January 2024

Unlike classic APS, CAPS causes multiple microthrombosis due to an increased inflammatory response, known as a “thrombotic storm”. CAPS typically develops after infection, trauma, or surgery and begins with the following symptoms: fever, thrombocytopenia, muscle weakness, visual and cognitive disturbances, abdominal pain, renal failure, and disseminated intravascular coagulation. Although the presence of antiphospholipid antibodies in the blood is one of the diagnostic criteria, the level of these antibodies can fluctuate significantly, which complicates the diagnostic process and can lead to erroneous interpretation of rapidly developing symptoms. Triple therapy is often used to treat CAPS, which includes the use of anticoagulants, plasmapheresis, and high doses of glucocorticosteroids and, in some cases, additional intravenous immunoglobulins. The use of LMWH is recommended as the drug of choice due to its anti-inflammatory and anticoagulant properties. CAPS is a multifactorial disease that requires not only an interdisciplinary approach but also highly qualified medical care, adequate and timely diagnosis, and appropriate prevention in the context of relapse or occurrence of the disease. Improved new clinical protocols and education of medical personnel regarding CAPS can significantly improve the therapeutic approach and reduce mortality rates.
Pulmonary Hypertension in Underrepresented Minorities: A Narrative Review
Authors: Contreras Johanna , Nussbaum Jeremy , Cangialosi Peter , Thapi Sahityasri , Radakrishnan Ankitha , Hall Jillian , Ramesh Prashasthi , Trivieri Maria Giovanna , Sandoval Alejandro Folch
Published: January 2024

Minoritized racial and ethnic groups suffer disproportionately from the incidence and morbidity of pulmonary hypertension (PH), as well as its associated cardiovascular, pulmonary, and systemic conditions. These disparities are largely explained by social determinants of health, including access to care, systemic biases, socioeconomic status, and environment. Despite this undue burden, minority patients remain underrepresented in PH research. Steps should be taken to mitigate these disparities, including initiatives to increase research participation, combat inequities in access to care, and improve the treatment of the conditions associated with PH.
Unlocking the Potential of Acetazolamide: A Literature Review of an Adjunctive Approach in Heart Failure Management
Authors: Sabina Michael , Barakat Zein , Feliciano Adrian , Lamb Andrew , Alsamman M Mrhaf
Published: January 2024

Background: Heart failure (HF) patients often experience persistent fluid overload despite standard diuretic therapy. The adjunctive use of acetazolamide, a carbonic anhydrase inhibitor, in combination with loop diuretics has shown promise in improving decongestion and diuretic efficacy. This literature review aims to analyze six studies evaluating the effectiveness of acetazolamide as an additive treatment for acute decompensated heart failure (ADHF) and its impact on various outcomes. Methods: We searched the PubMed database using the terms “acetazolamide heart failure”. We refined our search with specific filters (as shown our PRISMA flow diagram) and exclusion criteria, narrowing down our results to five studies. We included an extra study via expert recommendation, ultimately including six studies for comprehensive analysis. Results: The review highlights the positive effects of acetazolamide on decongestion, natriuresis, and diuresis in HF patients. However, it also showcases the limitations of these trials. Discussion: While the reviewed studies demonstrate the potential benefits of acetazolamide in enhancing decongestion and diuretic efficiency, there are limitations to consider, including small sample sizes, lack of blinding, and limited external validity. Further research is needed to confirm these findings, compare acetazolamide with other diuretic combinations, and explore its effects in a broader population of heart failure patients, including those in the United States. The use of acetazolamide in HF management warrants continued investigation to optimize its role in improving decongestion and patient outcomes.
Considering Histologic Remission in Ulcerative Colitis as a Long-Term Target
Authors: Pavel Christopher , Diculescu Mircea Mihai , Stepan Alex-Emilian , Constantinescu Gabriel , Sandru Vasile , Ţieranu Cristian George , Tomescu Luiza , Constantinescu Alexandru , Patoni Cristina , Plotogea Oana-Mihaela , Ilie Madalina
Published: January 2024

Monitoring disease activity in inflammatory bowel disease (IBD) is challenging since clinical manifestations do not represent reliable surrogates for an accurate reflection of the inflammatory burden. Endoscopic remission had been the most significant endpoint target in the last years; nevertheless, a remarkable proportion of patients continue to relapse despite a normal-appearing mucosa, highlighting that endoscopy may underestimate the true extent of the disease. A subtle hint of the importance that histology plays in the long-term course of the disease has been endorsed by the STRIDE-II consensus, which recommends considering histologic healing for ulcerative colitis (UC), even though it is not stated to be a compulsory formal target. It is a continuum-changing paradigm, and it is almost a certainty that in the near future, histologic healing may become the new formal target for ulcerative colitis. It must be emphasized that there is great heterogeneity in defining histological remission, and the main criteria or cut-off values for inflammatory markers are still in an ill-defined area. The complexity of some histologic scores is a source of confusion among clinicians and pathologists, leading to low adherence in clinical practice when it comes to a homogenous histopathological report. Therefore, a standardized and more practical approach is urgently needed.
Relationship between Biochemical Pathways and Non-Coding RNAs Involved in the Progression of Diabetic Retinopathy
Authors: Mrowicka Małgorzata , Mrowicki Jerzy , Majsterek Ireneusz
Published: January 2024

Diabetic retinopathy (DR) is a progressive blinding disease, which affects the vision and quality of life of patients, and it severely impacts the society. This complication, caused by abnormal glucose metabolism, leads to structural, functional, molecular, and biochemical abnormalities in the retina. Oxidative stress and inflammation also play pivotal roles in the pathogenic process of DR, leading to mitochondrial damage and a decrease in mitochondrial function. DR causes retinal degeneration in glial and neural cells, while the disappearance of pericytes in retinal blood vessels leads to alterations in vascular regulation and stability. Clinical changes include dilatation and blood flow changes in response to the decrease in retinal perfusion in retinal blood vessels, leading to vascular leakage, neovascularization, and neurodegeneration. The loss of vascular cells in the retina results in capillary occlusion and ischemia. Thus, DR is a highly complex disease with various biological factors, which contribute to its pathogenesis. The interplay between biochemical pathways and non-coding RNAs (ncRNAs) is essential for understanding the development and progression of DR. Abnormal expression of ncRNAs has been confirmed to promote the development of DR, suggesting that ncRNAs such as miRNAs, lncRNAs, and circRNAs have potential as diagnostic biomarkers and theranostic targets in DR. This review provides an overview of the interactions between abnormal biochemical pathways and dysregulated expression of ncRNAs under the influence of hyperglycemic environment in DR.
Follow-up for More than 10 Years of Patients with Peritoneal Metastases Treated with Cytoreductive Surgery + Hyperthermic Intraperitoneal Chemotherapy in a Specialized Unit
Authors: Fernández-Candela Alba , Bretcha-Boix Pedro , Ruíz Ramírez Juan Carlos , Paz Alejandro , Munoz Paula , Ortega Miguel A. , Álvarez-Mon Melchor , Farré-Alegre José
Published: January 2024

Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) have demonstrated their impact on disease-free survival (DFS) and overall survival (OS) of patients with peritoneal metastases (PM). However, prior literature lacks evidence regarding any follow-up beyond 5 years. In this study, we analyse long-term OS and DFS (more than 10 years of follow-up) of patients undergoing CRS + HIPEC in a specialized unit. We conducted a retrospective study that included only patients who underwent CRS + HIPEC from January 2001 to May 2012. Data collection was conducted by reviewing medical records and telephone calls to patients or relatives. A total of 86 patients were included. The mean PCI was nine (range 0–39) and complete cytoreduction (CC-0) was reached in 80% of patients. Postoperative complications Clavien–Dindo III-IV occurred in 27.9% of patients and the 30-day mortality rate was 2.3%. After 10 years of actual follow-up, OS was 33.7% and DFS was 31.4%. Considering the historical context in which the standard of care for patients with PM was palliation, the results obtained show that CRS + HIPEC was a valid option, with morbimortality comparable to other major abdominal surgeries and encouraging survival results, since, after 10 years of follow-up, almost one-third of patients are still alive and disease-free.
Multi-Ported GC-eDRAM Bitcell with Dynamic Port Configuration and Refresh Mechanism
Authors: Golman Roman , Giterman Robert , Teman Adam
Published: January 2024

Embedded memories occupy an increasingly dominant part of the area and power budgets of modern systems-on-chips (SoCs). Multi-ported embedded memories, commonly used by media SoCs and graphical processing units, occupy even more area and consume higher power due to larger memory bitcells. Gain-cell eDRAM is a high-density alternative for multi-ported operation with a small silicon footprint. However, conventional gain-cell memories have limited data availability, as they require periodic refresh operations to maintain their data. In this paper, we propose a novel multi-ported gain-cell design, which provides up-to N read ports and M independent write ports (NRMW). In addition, the proposed design features a configurable mode of operation, supporting a hidden refresh mechanism for improved memory availability, as well as a novel opportunistic refresh port approach. An 8kbit memory macro was implemented using a four-transistor bitcell with four ports (2R2W) in a 28 nm FD-SOI technology, offering up-to a 3× reduction in bitcell area compared to other dual-ported SRAM memory options, while also providing 100% memory availability, as opposed to conventional dynamic memories, which are hindered by limited availability.
The Role of Vegetation Monitoring in the Conservation of Coastal Habitats N2000: A Case Study of a Wetland Area in Southeast Sicily (Italy)
Authors: Sciandrello Saverio , Ranno Veronica , Tomaselli Valeria
Published: January 2024

The coastal wetlands are among the most vulnerable and threatened environments of the Mediterranean area. Targeted actions for their conservation require in-depth knowledge of current and past natural vegetation. In this paper, we surveyed the vegetation composition and the spatio-temporal changes of a coastal wetland area in southeastern Sicily (“Saline di Priolo” SAC). Based on 128 phytosociological surveys and several plant collections, a total of 304 taxa, 28 plant communities, and 16 habitats have been identified. Furthermore, three new plant associations were described, including two in wetland and one in rocky coast environments. For the classification of plant communities and habitats, a hierarchical clustering was performed by using Euclidean coefficient and beta-flexible algorithm. The life form spectrum of the current flora highlights the dominance of therophytes and hemicryptophytes. The Mediterranean species are largely prevailing with 123 taxa. The cartographic analysis performed with ArcGis 10.3 shows a radical reduction in the wetland habitats in the last 70 years, and a strong alteration of the ecological succession of the psammophilous-hygrophilous vegetation. Moreover, landscape configuration of the coastal vegetation and habitat types was well highlighted by a set of specific landscape metrics. In particular, our outcomes identify three habitats (2110, 2210, and 5220* EU code) with bad conservation status, among which we identified one of priority conservation (Zyziphus arborescent matorral) that requires urgent restoration measures.
Analysis of Land Suitability for Maize Production under Climate Change and Its Mitigation Potential through Crop Residue Management
Authors: Karapetsas Nikolaos , Gobin Anne , Bilas George , Koutsos Thomas M. , Pavlidis Vasileios , Katragkou Eleni , Alexandridis Thomas K.
Published: January 2024

Land Suitability Analysis (LSA), under the impact of climate change, is a fundamental approach to the design of appropriate land management strategies for sustainable crop production and food security. In this study, the FAO framework was used to assess the impact of climate change on land suitability for maize in Flanders, Belgium. The current LSA revealed the marginal suitability for maize cultivation, characterizing most of the agricultural land in Flanders and identifying precipitation as the most limiting factor for maize suitability. The LSA, under two climate change scenarios, was based on climate projections from several CMIP5 Global Circulation Models, transformed into future land suitability projections and assembled into a multi-model ensemble (MME) of projected suitability changes. The results indicate an average reduction in projected suitability of approximately 7% by 2099 under the high-emission scenario. The potential of the Soil-Improving Cropping System (SICS) to mitigate the impacts of climate change on land suitability was statistically significant under both low- and high-emission scenarios. This research provides valuable insights into the MME modeling of climate change impacts on land suitability and its associated uncertainty, with the application of SICS as a potential long-term mitigation measure to promote sustainable agricultural practices.
Impact of Acute Kidney Injury on the COVID-19 In-Hospital Mortality in Octogenarian Patients: Insights from the COVOCA Study
Authors: Caturano Alfredo , Galiero Raffaele , Vetrano Erica , Medicamento Giulia , Alfano Maria , Beccia Domenico , Brin Chiara , Colantuoni Sara , Di Salvo Jessica , Epifani Raffaella , Nevola Riccardo , Marfella Raffaele , Sardu Celestino , Coppola Carmine , Scarano Ferdinando , Maggi Paolo , Calabrese Cecilia , De Lucia Sposito Pellegrino , Rescigno Carolina , Sbreglia Costanza , Fraganza Fiorentino , Parrella Roberto , Romano Annamaria , Calabria Giosuele , Polverino Benedetto , Pagano Antonio , Numis Fabio Giuliano , Bologna Carolina , Nunziata Mariagrazia , Esposito Vincenzo , Coppola Nicola , Maturo Nicola , Nasti Rodolfo , Di Micco Pierpaolo , Perrella Alessandro , Adinolfi Luigi Elio , Di Domenico Marina , Monda Marcellino , Russo Vincenzo , Ruggiero Roberto , Docimo Giovanni , Rinaldi Luca , Sasso Ferdinando Carlo
Published: January 2024

Background and Aims: The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has fundamentally reshaped the landscape of global public health, with some people suffering more adverse clinical outcomes than others. The aim of this study is to deepen our understanding of the specific impact of acute kidney injury (AKI) on the in-hospital mortality in octogenarian patients with COVID-19. Methods: This is a prospective observational cohort study, which involved 23 COVID-19 hospital units in the Campania Region, Italy. Exposure variables were collected during hospital admission and at discharge. Only patients aged ≥80 years were deemed eligible for the study. Results: 197 patients were included in the study (median age 83.0 [82.0–87.0] years; 51.5% men), with a median duration of hospitalization of 15.0 [8.0–25.0] days. From the multivariable Cox regression analysis, after the application of Šidák correction, only the respiratory rate (HR 1.09, 95% CI: 1.04 to 1.14; p < 0.001) and AKI development (HR: 3.40, 95% CI: 1.80 to 6.40; p < 0.001) were independently associated with the primary outcome. Moreover, the Kaplan–Meier analysis showed a significantly different risk of in-hospital mortality between patients with and without AKI (log-rank: <0.0001). Conclusions: In our investigation, we identified a significant association between AKI and mortality rates among octogenarian patients admitted for COVID-19. These findings raise notable concerns and emphasize the imperative for vigilant monitoring of this demographic cohort.
Geofencing Motion Planning for Unmanned Aerial Vehicles Using an Anticipatory Range Control Algorithm
Authors: Thomas Peter R. , Sarhadi Pouria
Published: January 2024

This paper presents a range control approach for implementing hard geofencing for unmanned air vehicles (UAVs), and especially remotely piloted versions (RPVs), via a proposed anticipatory range calculator. The approach employs turning circle intersection tests that anticipate the fence perimeter on approach. This ensures the vehicle turns before penetrating the geofence and remains inside the allowable operational airspace by accounting for the vehicles’ turning dynamics. Allowance is made for general geozone shapes and locations, including those located at the problematic poles and meridians where nonlinear angle mapping is dealt with, concave geozones, narrow corners with acute internal angles, and transient turn dynamics. The algorithm is shown to prevent any excursions using a high-fidelity simulation of a small remotely piloted vehicle. The algorithm relies on a single tuning parameter which can be determined from the closed-loop rise time in the aircraft’s roll command tracking.
Modeling Study of Factors Determining Efficacy of Biological Control of Adventive Weeds
Authors: Tyutyunov Yuri V. , Govorukhin Vasily N. , Tsybulin Vyacheslav G.
Published: January 2024

We model the spatiotemporal dynamics of a community consisting of competing weed and cultivated plant species and a population of specialized phytophagous insects used as the weed biocontrol agent. The model is formulated as a PDE system of taxis–diffusion–reaction type and computer-implemented for one-dimensional and two-dimensional cases of spatial habitat for the Neumann zero-flux boundary condition. In order to discretize the original continuous system, we applied the method of lines. The obtained system of ODEs is integrated using the Runge–Kutta method with a variable time step and control of the integration accuracy. The numerical simulations provide insights into the mechanism of formation of solitary population waves (SPWs) of the phytophage, revealing the factors that determine the efficacy of combined application of the phytophagous insect (classical biological method) and cultivated plant (phytocenotic method) to suppress weed foci. In particular, the presented results illustrate the stabilizing action of cultivated plants, which fix the SPW effect by occupying the free area behind the wave front so that the weed remains suppressed in the absence of a phytophage.
Cohomology and Deformations of Relative Rota–Baxter Operators on Lie-Yamaguti Algebras
Authors: Zhao Jia , Qiao Yu
Published: January 2024

In this paper, we establish the cohomology of relative Rota–Baxter operators on Lie-Yamaguti algebras via the Yamaguti cohomology. Then, we use this type of cohomology to characterize deformations of relative Rota–Baxter operators on Lie-Yamaguti algebras. We show that if two linear or formal deformations of a relative Rota–Baxter operator are equivalent, then their infinitesimals are in the same cohomology class in the first cohomology group. Moreover, an order n deformation of a relative Rota–Baxter operator can be extended to an order n+1 deformation if and only if the obstruction class in the second cohomology group is trivial.
Phase-Dependent Adsorption of Myelin Basic Protein to Phosphatidylcholine Lipid Bilayers
Authors: Maleš Petra , Brkljača Zlatko , Crnolatac Ivo , Petrov Dražen , Bakarić Danijela
Published: January 2024

The dense packing of opposite cytoplasmic surfaces of the lipid-enriched myelin membrane, responsible for the proper saltatory conduction of nerve impulses through axons, is ensured by the adhesive properties of myelin basic protein (MBP). Although preferentially interacting with negatively charged phosphatidylserine (PS) lipids, as an intrinsically disordered protein, it can easily adapt its shape to its immediate environment and thus adsorb to domains made of zwitterionic phosphatidylcholine (PC) lipids. As the molecular-level interaction pattern between MBP and PC lipid membranes suffers from scarce characterization, an experimental and computational study of multilamellar liposomes (MLVs) composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in the presence of bovine MBP is presented here. Calorimetric and temperature-dependent UV-Vis measurements identified DPPC pretransition temperature (Tp) and calorimetric enthalpy (ΔHcal) as the physicochemical parameters most responsive to the presence of MBP. Besides suggesting an increase in β-sheet fractions of structured MBP segments as DPPC lipids undergo from the gel (20 °C) to the fluid (50 °C) phase, FTIR spectra unraveled the significant contribution of lysine (Lys) residues in the adsorption pattern, especially when DPPC is in the fluid (50 °C) phase. In addition to highlighting the importance of Lys residues in the MBP adsorption on DPPC lipid bilayer, employing salt bridges (SBs) and hydrogen bonds (HBs), MD data suggest the crucial importance of the orientation of MBP with respect to the surface of the DPPC lipid bilayer.
A Simple Calibration Method to Consider Plastic Deformation Influence on X-ray Elastic Constant Based on Peak Width Variation
Authors: Gautier Ewann , Faucheux Pierre , Levieil Bruno , Barrallier Laurent , Calloch Sylvain , Doudard Cédric
Published: January 2024

The sin²⁡ψ method is the general method for analyzing X-ray diffraction stress measurements. This method relies on the estimation of a parameter known as 12S2hkl, which is generally considered as a material constant. However, various studies have shown that this parameter can be affected by plastic deformation leading to proportional uncertainties in the estimation of stresses. In this paper, in situ X-ray diffraction measurements are performed during a tensile test with unloads on a low-carbon high-strength steel. The calibrated 12S2hkl parameter varies from 3.5×10−6 MPa−1 to 5.5 ×10−6 Mpa−1, depending on the surface condition and on the plastic strain state, leading to a maximum error on the stress level of 40% compared to reference handbook values. The results also show that plastic strain is responsible for 6 to 14% of the variation, depending on the initial surface sample condition. A method is then proposed to correct this variation based on the fit of the 12S2hkl evolution with respect to the peak diffraction width, the latter being an indication of the plasticity state. It is shown that the proposed methodology improves the applied stress increment prediction, although the absolute stress value still depends on pseudo-macrostresses that also vary with plastic strain.
Coupling Effect of Mn Addition and Deformation on Mechanical and Electrical Properties of Al-Zr Alloys
Authors: Wang Ruihong , Lai Yulei , Liu Bilong , Chen Bao’an
Published: January 2024

In order to increase the strength of Al-Zr alloys, which are promisingly used for heat-resistant conductors, the coupling effect of Mn addition (0.16 wt.% and 0.88 wt.%) and deformation on the precipitation, mechanical, and electrical properties of an Al-0.18wt.% Zr alloy was studied using transmission electron microscopy (TEM), atom probe tomography (APT), hardness testing, and electrical conductivity measurement, respectively. Results showed that the Mn addition fully suppresses the Al3Zr precipitation in both hot-deformed and undeformed cases, which is mainly due to a strong Mn-vacancy bonding, in which Mn atoms seize vacancies and hence reduce the available vacancies for Al3Zr nucleation. Minor 0.16 wt.% Mn addition causes a simultaneous decrease in hardness and electrical conductivity, regardless of whether there is deformation. The higher 0.88 wt.% Mn addition, however, significantly increases the hardness by over 40%, especially in combination with deformation. Possible influencing factors such as grain size, dislocations, intergranular/intragranular precipitation, and solute clusters are comparatively discussed in terms of microstructural features and mechanical/electrical properties that are tuned by Mn addition and/or deformation. It is found that the Mn addition can make remarkable contributions to the hardness and thermal stability of the Al-Zr alloys when coupled with deformation.
Analysis of Flow Characteristics between Tandem Flexible Structures Based on PIV: Substantial Applications for the Removal of Microplastics
Authors: Lee Hyeonjin , Sangtam Bongliba T. , Seong Heejoong , Kim Jeong Jae , Park Hanwook
Published: January 2024

This study emphasizes the potential risk posed by microplastics, particularly in tap water. Numerous studies have reported the removal of microplastics, but the limitations in addressing this issue remain challenging. To tackle this problem, a new method is introduced using tandem flexible structures (FSs) for microplastic removal. The present study focused on understanding the hydrodynamic characteristics between FSs to utilize microplastic removal. This comprehension of fluid flow and FSs offers valuable insights for improving the efficiency of microplastic removal methods. Therefore, the optimal conditions for removing microplastics were experimentally investigated inside the FSs gap region. Based on the gap distance and height, the flow structures between FSs were investigated. A small secondary vortex structure that could trap particles from upstream was continuously maintained behind the upstream FSs under certain geometric conditions. It is shown that this vortex structure has an effective way of confining the particles from upstream. The persistency of a small secondary vortex was also evaluated. This study may be helpful to researchers working on microplastic removal and FSs with a tandem arrangement.
Insights into the Subduction of the Ligure-Piemontese Oceanic Basin: New Constraints from the Metamorphism in the Internal Ligurian Units (Northern Apennines, Italy)
Authors: Sanità Edoardo , Di Rosa Maria , Marroni Michele , Meneghini Francesca , Pandolfi Luca
Published: January 2024

In the Northern Apennines, the Internal Ligurian Units are considered deformed and metamorphosed fragments of the Ligure-Piemontese oceanic basin. In this paper, we report on the temperature and pressure conditions of the metamorphic peak for four Internal Ligurian Units, estimated using different geothermometers and geobarometers based on the white mica and chlorite compositions. These minerals were formed during the D1 deformation phase in the pre-Oligocene. The results indicate that the Portello and Gottero units are both characterized by metamorphic conditions pertaining to low blueschists facies, while the Colli-Tavarone and Bracco-Val Graveglia Units show a lower metamorphic imprint that produces assemblages of prehnite-pumpellyite facies. The estimated geothermal gradient for the metamorphic peak achieved by the analyzed Internal Ligurian Units during the D1 phase is 7–15 °C/Km, which is indicative of deformation in a subduction setting. Under these conditions, the D1 phase developed in these units as a result of underplating at the base of the accretionary wedge during the closure of the Ligure-Piemontese basin. These data indicate a close geodynamic correlation among the Internal Ligurian Units and the ophiolite-bearing units of the Alps.
Post-Collisional Tectonomagmatic Evolution, Crustal Reworking and Ore Genesis along a Section of the Southern Variscan Belt: The Variscan Mineral System of Sardinia (Italy)
Authors: Naitza Stefano , Casini Leonardo , Cocco Fabrizio , Deidda Matteo Luca , Funedda Antonio , Loi Alfredo , Oggiano Giacomo , Secchi Francesco
Published: January 2024

Since the early Paleozoic, numerous metallogenic events produced in the Sardinian massif a singular concentration of mineral deposits of various kinds. Among them, the Variscan metallogenic peak represents a late Paleozoic phase of diffuse ore formation linked to the tectonomagmatic evolution of the Variscan chain. Two main classes of ores may primarily be attributed to this peak: (1) mesothermal orogenic-type As-Au ± W ± Sb ores, only found in E Sardinia, and (2) intrusion-related Sn-W-Mo-F and base metals-bearing ores found in the whole Sardinian Batholith, but mainly occurring in central–south Sardinia. Both deposit classes formed diachronously during the Variscan post-compressional extension. The orogenic-type ores are related to regional-scale flows of mineralizing fluids, and the intrusion-related ores occur around fertile intrusions of different granite suites. Metallogenic reconstructions suggest almost entirely crustal processes of mineralization without a significant contribution from the mantle. We summarized these processes with a holistic approach and conceptualized the Sardinian Variscan Mineral System (SVMS), a crustal-scale physical system of ore mineralization in the Sardinian basement. The SVMS required suitable metal sources in the crust and diffuse crustal reworking triggered by heat that allowed (a) the redistribution of the original metal budget of the crust in magmas by partial melting and (b) the production of metal-bearing fluids by metamorphic dehydration. Heat transfer in the Sardinian Variscan crust involved shear heating in lithospheric shear zones and the role of mantle uplift as a thermal engine in an extensional tectonic setting. Lithospheric shear zones acted as effective pathways in focusing fluid flow through a large-scale plumbing system into regional-scale structural traps for ores. Pre-Variscan metal sources of metallogenic relevance may have been (1) the magmatic arc and magmatic arc-derived materials of Ordovician age, extensively documented in E Sardinia crust, and (2) an inferred Precambrian crystalline basement lying under the Phanerozoic crustal section, whose presence has been assumed from geophysical data and from petrological and geochemical characteristics of granite suites. At shallower crustal levels, important contributions of metals may have come from pre-Variscan ore sources, such as the Pb-Zn MVT Cambrian ores of SW Sardinia or the REE-bearing Upper Ordovician paleoplacers of E Sardinia.
Correction: Blanco et al. Synthesis and Characterization of [Fe(Htrz)2(trz)](BF4)] Nanocubes. Molecules 2022, 27, 1213
Authors: Blanco Alexis A. , Adams Daniel J. , Azoulay Jason D. , Spinu Leonard , Wiley John B.
Published: January 2024

After publication of the paper [...]
DNA-Based Fluorescent Nanoprobe for Cancer Cell Membrane Imaging
Authors: Wu Xiaoqiao , Shuai Xinjia , Nie Kunhan , Li Jing , Liu Lin , Wang Lijuan , Huang Chengzhi , Li Chunmei
Published: January 2024

As an important barrier between the cytoplasm and the microenvironment of the cell, the cell membrane is essential for the maintenance of normal cellular physiological activities. An abnormal cell membrane is a crucial symbol of body dysfunction and the occurrence of variant diseases; therefore, the visualization and monitoring of biomolecules associated with cell membranes and disease markers are of utmost importance in revealing the biological functions of cell membranes. Due to their biocompatibility, programmability, and modifiability, DNA nanomaterials have become increasingly popular in cell fluorescence imaging in recent years. In addition, DNA nanomaterials can be combined with the cell membrane in a specific manner to enable the real-time imaging of signal molecules on the cell membrane, allowing for the real-time monitoring of disease occurrence and progression. This article examines the recent application of DNA nanomaterials for fluorescence imaging on cell membranes. First, we present the conditions for imaging DNA nanomaterials in the cell membrane microenvironment, such as the ATP, pH, etc. Second, we summarize the imaging applications of cell membrane receptors and other molecules. Finally, some difficulties and challenges associated with DNA nanomaterials in the imaging of cell membranes are presented.
Borane-Pyridine: An Efficient Catalyst for Direct Amidation
Authors: Ramachandran P. Veeraraghavan , Singh Aman , Walker Harry , Hamann Henry J.
Published: January 2024

Borane-pyridine acts as an efficient (5 mol%) liquid catalyst, providing improved solubility for the direct amidation of a wide range of aromatic and aliphatic carboxylic acids and amines to form secondary and tertiary carboxamides. Tolerance of potentially incompatible halo, nitro, and alkene functionalities has been demonstrated.
Variability of the Cervical Branch Depending on the Facial Nerve Branching Pattern and Anthropometric Type of the Head
Authors: Babuci Angela , Palarie Victor , Catereniuc Ilia , Zorina Zinovia , Visnevschi Sergiu , Heimes Diana , Lehtman Sofia , Kämmerer Peer W.
Published: January 2024

(1) Background: Considering that the specialty literature supplies only general data about the variability of the cervical branch of the facial nerve, this study aimed to determine this branch’s variation and individual peculiarities depending on the nerve branching pattern and anthropometric type of the head. (2) Methods: The study was conducted on 75 hemifaces of adult formalized cadavers. Ahead of anatomical dissection, each head was measured to establish the anthropometric type, according to Franco and colleagues. The branching patterns were then distributed according to the Davis classification. (3) Results: The number of cervical branches (CB) of the facial nerve varied from one to five branches, with the following rate: 1 CB (61.3%), 2 CB (28%), 3 CB (6.7%), 4 CB (2.7%), and 5 CB (1.3%). Seven branching patterns of the facial nerve were revealed: Type I in 18.7%, Type II in 14.7%, Type III in 20%, Type IV in 14.6%, Type V in 5.3%, Type VI in 18.7%, and Type NI in 8% (bizarre types). According to the branching pattern, the mean numbers of the cervical branches were as follows: Type I—1.6 ± 1.02; Type II—1.4 ± 0.50; Type III—1.4 ± 0.50; Type IV—1.4 ± 0.67; Type V—2.0 ± 1.41; Type VI—1.8 ± 1.12; and Type-NI—1.8 ± 0.75; p = 0.599. According to the anthropometric type of the head, the mean number of CB in the mesocephalic type (MCT) was 1.5 ± 0.82, in the dolichocephalic type (DCT), 1.7 ± 0.87, and in the brachycephalic type, (BCT) 1.8 ± 1.04; p = 0.668. (4) Conclusions: The cervical branch of the facial nerve varies depending on the facial nerve branching pattern and the anthropometric type of the head. The highest degree of variation was characteristic of BCT and Type V and the lowest, of MCT and Types II, III, and IV.
Flavonoid Metabolites in Serum and Urine after the Ingestion of Selected Tropical Fruits
Authors: Chomphen Lalita , Yamanont Paveena , Morales Noppawan Phumala
Published: January 2024

The serum concentration and urinary excretion of flavonoids after the ingestion of guava, pineapple, and pomelo were determined using liquid chromatography–mass spectroscopy (LC-MS/MS). Each group of healthy volunteers was given 200 g of fresh fruit after overnight fasting and a 24-h flavonoid-free diet. The results demonstrate that only the glucuronic-conjugated metabolites of luteolin, quercetin, kaempferol, and myricetin were detected after fruit ingestion. The metabolites were first detected after 2 h, with the time to maximum concentration (Tmax) at 6 h. The most abundant metabolites for guava, pineapple, and pomelo were the glucuronide metabolites of quercetin (AUC0–8 5.4 ± 1.3 μg·h/mL), kaempferol (AUC0–8 9.9 ± 2.3 μg·h/mL), and luteolin (AUC0–8 6.4 ± 1.1 μg·h/mL), respectively. The flavonoids found in the 24-h urinary excretions were glucuronic- and mainly sulfate-conjugated metabolites. Quercetin metabolites were the most abundant after guava and pineapple ingestion, accounting for 900 and 700 μg, respectively. Luteolin metabolites were the most abundant after pomelo ingestion, accounting for 450 μg. The serum and urinary metabolite profiles suggested that guava and pineapple are good sources of quercetin, pineapple is a good source of kaempferol, and pomelo is a good source of luteolin. The study of flavonoid profiles may provide information for the selection of fruits as functional foods for their health benefits to help with various health conditions.
Effects of a Singular Dose of Mangiferin–Quercetin Supplementation on Basketball Performance: A Double-Blind Crossover Study of High-Level Male Players
Authors: Bourdas Dimitrios I. , Travlos Antonios K. , Souglis Athanasios , Stavropoulou Georgia , Zacharakis Emmanouil , Gofas Dimitrios C. , Bakirtzoglou Panteleimon
Published: January 2024

Pre-exercise mangiferin–quercetin may enhance athletic performance. This study investigated the effect of mangiferin–quercetin supplementation on high-level male basketball players during a basketball exercise simulation test (BEST) comprising 24 circuits of 30 s activities with various movement distances. The participants were divided into two groups (EXP = 19 and CON = 19) and given a placebo one hour before the BEST (PRE-condition). The following week, the EXP group received mangiferin–quercetin (84 mg/140 mg), while the CON group received a placebo (POST-condition) before the BEST in a double-blind, cross-over design. The mean heart rate (HR) and circuit and sprint times (CT and ST) during the BEST were measured, along with the capillary blood lactate levels (La−), the subjective rating of muscle soreness (RPMS), and the perceived exertion (RPE) during a resting state prior to and following the BEST. The results showed significant interactions for the mean CT (p = 0.013) and RPE (p = 0.004); a marginal interaction for La− (p = 0.054); and non-significant interactions for the mean HR, mean ST, and RPMS. Moreover, the EXP group had significantly lower values in the POST condition for the mean CT (18.17 ± 2.08 s) and RPE (12.42 ± 1.02) compared to the PRE condition (20.33 ± 1.96 s and 13.47 ± 1.22, respectively) and the POST condition of the CON group (20.31 ± 2.10 s and 13.32 ± 1.16, respectively) (p < 0.05). These findings highlight the potential of pre-game mangiferin–quercetin supplementation to enhance intermittent high-intensity efforts in sports such as basketball.
Antibacterial Effect of Sesame Protein-Derived Peptides against Escherichia coli and Staphylococcus aureus: In Silico and In Vitro Analysis
Authors: Zhu Zehui , Pan Fei , Wang Ou , Zhao Liang , Zhao Lei
Published: January 2024

This study aimed to screen out antibacterial peptides derived from sesame (Sesamum indicum L.) through in silico and in vitro methods. In silico proteolysis of sesame proteins with pepsin, trypsin, and chymotrypsin was performed with the online server BIOPEP-UWM. The CAMPR3 online server was used to predict the antimicrobial effect of peptides. The ToxinPred, PepCalc, and AllergenFP tools were utilized to forecast the physicochemical properties, toxicity, and allergen of the peptides. Molecular docking analysis showed that six cationic antimicrobial peptides could directly interact with the key sites of dihydropteroate synthase, whereas Ala-Gly-Gly-Val-Pro-Arg and Ser-Thr-Ile-Arg exhibited the strongest binding affinity. In vitro antibacterial experiment showed the minimum inhibitory concentration (MIC) of Ser-Thr-Ile-Arg against Escherichia coli and Staphylococcus aureus was 1024 and 512 µg/mL, respectively. Meanwhile, MIC of Ala-Gly-Gly-Val-Pro-Arg against both bacterial species was 512 µg/mL. Our results suggest that peptides from sesame possess the ability to potentially hinder bacterial activity.
The Characterisation of Lactococcus garvieae Isolated in an Outbreak of Septicaemic Disease in Farmed Sea Bass (Dicentrarchus labrax, Linnaues 1758) in Italy
Authors: Salogni Cristian , Bertasio Cristina , Accini Adelchi , Gibelli Lucia Rita , Pigoli Claudio , Susini Francesca , Podavini Eleonora , Scali Federico , Varisco Giorgio , Alborali Giovanni Loris
Published: January 2024

In aquaculture, Lactococcus garvieae is a common fish pathogen that can cause significant economic losses in several fresh and saltwater species. Despite the extensive range of hosts, L. garvieae infection in sea bass (Dicentrarchus labrax) has rarely been reported. During the summer of 2023, an outbreak occurred in an inland farm in the Gulf of Follonica (Tuscany, Italy). Fish of various sizes were affected, showing apathy, inappetence, erratic swimming and eye lesions, while the mortality was low (2–3% per month). Anatomopathological examinations suggested a septicaemic infection characterised by melanosis, diffuse redness (skin and fins), paleness (gills and internal organs), haemorrhages and splenomegaly. Seventy swabs from the viscera of 14 subjects were collected and colonies similar to Streptococcus spp. grew from all the samples. Lactococcus garvieae was identified via the biochemical tests, API20STREP, MALDI-TOF, 16S rDNA and whole genome sequencing. Genetical characterisation revealed remarkable differences between this isolate and the strains previously isolated in Italian fish farms. Feed treatments with flumequine and erythromycin were ineffective. Considering the limited effects of antimicrobials, preventive measures, such as vaccination and biosecurity, should be implemented.
New Formulation–Microporation Combination Approaches to Delivering Ciclopirox across Human Nails
Authors: Kishishita Juliana , de Almeida Perez Pimenta Camila , Cerqueira Macedo Danielle Patricia , Delgado-Charro M. Begoña , Bastos Leal Leila
Published: January 2024

Topical treatments for onychomycosis are of interest to those seeking to avoid systemic drug interactions and to improve systemic safety. This work aimed to develop aqueous-based, simple, and cost-effective vehicles that provide high solubility for ciclopirox and enable the delivery of an active through channels created by nail microporation. Following solubility tests, aqueous gels and thermogels based on hydroxypropylmethylcellulose and poloxamer 407, respectively, were loaded with 8% and 16% ciclopirox. Their performance was then compared to the marketed lacquer Micolamina® in in vitro release tests with artificial membranes and in in vitro permeation tests with human nail clippings with and without poration. Finally, a microbiological assay compared the best gel formulations and the reference product. Little correlation was observed between the in vitro release and the permeation data, and the drug release was highly membrane-dependent. Ciclopirox nail retention in single-dose, porated nails tests was larger than in daily-dosing, non-porated nail conditions. The series of new gel and thermogel vehicles delivered ciclopirox more effectively than Micolamina® in single-dose, porated nail experiments. The inhibition of Trichophyton rubrum activity was significantly increased with microporated nails when the gel formulations were applied but not with Micolamina®. Overall, the results suggest that the new vehicles could be successfully combined with nail microporation to improve the drug delivery and efficacy of topical antifungal medication while reducing the dosing frequency, facilitating patients’ adherence.
Innovative Approach to Enhance Bioavailability of Birch Bark Extracts: Novel Method of Oleogel Development Contrasted with Other Dispersed Systems
Authors: Andze Laura , Vitolina Sanita , Berzins Rudolfs , Rizikovs Janis , Godina Daniela , Teresko Arturs , Grinberga Solveiga , Sevostjanovs Eduards , Cirule Helena , Liepinsh Edgars , Paze Aigars
Published: January 2024

Birch outer bark extract (BBE), containing pentacyclic triterpenes such as betulin, lupeol, and betulinic acid, is a widely recognized natural product renowned for its diverse pharmacological effects. However, its limited water solubility restricts its bioavailability. Therefore, the main objective is to enhance the bioavailability of BBE for pharmaceutical use. In this study, we aimed to develop a dispersion system utilizing a unique oleogel-producing method through the recrystallization of BBE from an ethanol solution in the oil phase. We generated an oleogel that demonstrates a notable 42–80-fold improvement in betulin and lupeol peroral bioavailability from BBE in Wistar rats, respectively. A physical paste-like BBE hydrogel developed with antisolvent precipitation showed a 16–56-fold increase in the bioavailability of betulin and lupeol from BBE in rat blood plasma, respectively. We also observed that the repeated administration of the BBE oleogel did not exhibit any toxicity at the tested dose (38.5 mg/kg betulin, 5.2 mg/kg lupeol, 1.5 mg/kg betulinic acid daily for 7 days). Betulin and betulinic acid were not detected in rat heart, liver, kidney, or brain tissues after the peroral administration of the oleogel daily for 7 days. Lupeol was found in rat heart, liver, and kidney tissues.
Self-Healing Properties of Water Tree Damage in Multilayered Shell–Core-Structured Microcapsules/Cross-Linked Polyethylene Composites
Authors: Zhu Bo , Sun Hao , Zhu Yaqi , He Shengkun , Han Ximu
Published: January 2024

To investigate the effect of the structure of microcapsules on the properties of cross-linked polyethylene (XLPE) composites, three XLPE specimens filled with multilayered shell–core-structured microcapsules are designed. In this paper, the microcapsules are first analyzed morphologically and chemically. In addition, the effect of the microcapsule structure on the typical electrical properties of the composites is explored. Finally, the self-healing ability of XLPE specimens filled with microcapsules is verified. The results show that the SiO2 on the surface of the trilayer shell–core microcapsules can make the microcapsules and the XLPE matrix have a better mechanical interlocking ability, which makes the typical properties of the trilayer shell–core microcapsules slightly better than those of the bilayer shell–core microcapsules. Moreover, when the bilayer shell–core or trilayer shell–core microcapsules rupture under the action of an electric field, the repair material reacts with the water tree under capillary action to consume the residual water while generating organic matter to fill in the cavity, thus repairing the damaged area of the water tree and ultimately achieving the self-healing of the composite water tree.
Anthocyanin-Loaded Polymers as Promising Nature-Based, Responsive, and Bioactive Materials
Authors: Rosales-Murillo S.S. , Sánchez-Bodón Julia , Hernández Olmos S.L. , Ibarra-Vázquez M.F. , Guerrero-Ramírez L.G. , Pérez-Álvarez L. , Vilas-Vilela J.L.
Published: January 2024

Anthocyanins are a specific group of molecules found in nature that have recently received increasing attention due to their interesting biological and colorimetric properties that have been successfully applied in several fields such as food preservation and biomedicine. Consequently, reviews devoted to a general overview of these flavonoids have proliferated in recent years. Meanwhile, the incorporation of anthocyanins into polymeric systems has become an interesting strategy to widen the applicability of these molecules and develop new smart and functional polymers in the above cited areas. However, anthocyanin-based polymers have been scarcely reviewed in the literature. Accordingly, this review aims to be a systematic summary of the most recent approaches for the incorporation of anthocyanins into macro-, micro-, or nanostructured polymers. Moreover, this work describes the fundamentals of the applicability of smart anthocyanin-based polymers and offers an updated review of their most interesting applications as sensors, biological regulators, and active materials.