bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of physical activity on surrogate markers of cardiovascular disease in the haemodialysis population
Authors: Hull Katherine L , Abell Lucy , Adenwalla Sherna F , Billany Roseanne E , Burns Stephanie , Burton James O , Churchward Darren , Graham-Brown Matthew P M , Gray Laura J , Highton Patrick , Lightfoot Courtney J , Said Rahma , Smith Alice C , Young Hannah M L , March Daniel S
Published: June 2024

Background

The haemodialysis (HD) population is sedentary, with substantial cardiovascular disease risk. In the general population, small increases in daily step count associate with significant reductions in cardiovascular mortality. This study explores the relationship between daily step count and surrogate markers of cardiovascular disease, including left ventricular ejection fraction (LVEF) and native T1 (a marker of diffuse myocardial fibrosis), within the HD population.

Methods

This was a post hoc analysis of the association between daily step count and metabolic equivalent of task (MET) and prognostically important cardiac magnetic resonance imaging parameters from the CYCLE-HD study (ISRCTN11299707). Unadjusted linear regression and multiple linear regression adjusted for age, body mass index, dialysis vintage, haemoglobin, hypertension and ultrafiltration volume were performed. Significant relationships were explored with natural cubic spline models with four degrees of freedom (five knots).

Results

A total of 107 participants were included [age 56.3 ± 14.1 years, 79 (73.8%) males]. The median daily step count was 2558 (interquartile range 1054–4352). There were significant associations between steps and LVEF (β = 0.292; P = .009) and steps and native T1 (β = −0.245; P = .035). Further modelling demonstrated most of the increase in LVEF occurred at up to 2000 steps/day and there was an inverse dose–response relationship between steps and native T1, with the most pronounced reduction in native T1 between ≈2500 and 6000 steps/day.

Conclusions

The results suggest an association between daily step count and parameters of cardiovascular health in the HD population. These findings support the recommendations for encouraging physical activity but are not the justification. Further research should evaluate whether a simple physical activity intervention improves cardiovascular outcomes in individuals receiving maintenance HD.
Early Intestinal Ultrasound in Severe Ulcerative Colitis Identifies Patients at Increased Risk of 1-Year Treatment Failure and Colectomy
Authors: Ilvemark Johan F K F , Wilkens Rune , Thielsen Peter , Dige Anders , Boysen Trine , Brynskov Jørn , Bjerrum Jacob T , Seidelin Jakob B
Published: June 2024

Background and Aims

Reliable and easily accessible objective markers of disease activity to predict long-term treatment outcomes in severe ulcerative colitis [UC] are missing. We aimed to investigate if intestinal ultrasound [IUS] might predict long-term outcomes in hospitalised patients with severe UC, treated with intravenous [IV] corticosteroids.

Methods

Hospitalised patients with severe UC and IUS inflammation (bowel wall thickness [BWT] > 3.0 mm) starting IV corticosteroids were recruited at three university hospitals in Denmark. IUS was performed before treatment and 48 ± 24 h, 6 ± 1 days, and 3 months after treatment initiation. Time until colectomy or need for new interventions was registered together with Mayo score at 3 months and partial Mayo score [pMayo] at 12 months. Follow-up time was 12 months.

Results

In the final analysis, 56 patients were included; 45 [80%] patients needed intervention, including nine colectomies, during the 12-month follow-up. After 48 ± 24 h, no patient with a BWT < 3 mm needed a colectomy, p = 0.04. BWT ≥ 4 mm showed an increased risk of colectomy {odds ratio 9.5 (95% confidence interval [CI] 1.5–186), p = 0.03}, whereas a BWT ≥ 3 mm showed an increased risk of intervention (3.6 [1.1–12.5], p = 0.03). A BWT ≥ 4 mm resulted in a significantly shorter time until both colectomy, p = 0.03, and treatment intensification (mean days 75 [95% CI 24–127] vs 176 [119–233], p = 0.005). However, neither IUS parameters nor pMayo score, C-reactive protein [CRP], haemoglobin, or p-albumin could predict remission at 3 and 12 months.

Conclusion

BWT, assessed at 48 h post intravenous corticosteroid initiation in patients hospitalised with severe UC, may identify patients with an increased risk of short- and long-term colectomy and predict a more aggressive short-term disease course.
Analysis of genetic and clinical characteristics of androgen insensitivity syndrome: a cohort study including 12 families
Authors: Yuan Zheng , Fan Lijun , Wang Yi , Li Lele , Ren Xiaoya , Sui Shengbin , Song Yanning , Cheng Ming , Cao Bingyan , Gong Chunxiu
Published: June 2024

Context

Androgen insensitivity syndrome (AIS) manifests itself as variable symptoms of under-virilization in patients with 46,XY disorders caused by androgen receptor (AR) gene variants. This large-sample study aimed to correlate the genotypes and phenotypes to the fertility of individuals.

Methods

This was a cohort study that analyzed the genetic and clinical characteristics of patients with AIS from a single center in China.

Results

The 117 patients were divided into 53 with complete AIS (CAIS) and 64 with partial AIS (PAIS). At their first visit, the median age was 1.83 years (0.92-4.17), and the external masculinization score was 3.0 (2.0-6.0). At the last follow-up, 92% (49/53) of patients with CAIS maintained their female gender, and 94% (60/64) of patients with PAIS were raised as males. No gender anxiety was observed in this study. Eighty-eight AR variants were identified, with 31 (35%) being unreported. Moreover, 24% (21/88) occurred more than once. The variants that appeared most frequently were located at amino acid 841, including p.R841H (n = 5) and p.R841C (n = 2). Variants p.N706S, p.R856H, and p.A871V were each observed 4 times. In terms of inheritance, 83% of patients with parental verification inherited variants from their mothers. We also observed that the variants from 1 case were inherited from his maternal grandfather who had hypospadias.

Conclusion

Most children with PAIS were raised as males. The abundance of maternally inheritable variants and the presence of case of preserved fertility indicate the fertility potential in patients with AIS. Hence, we recommend a careful evaluation of gonadectomy when fertility preservation is being considered.
Life history traits and cancer prevalence in birds
Authors: Kapsetaki Stefania E , Compton Zachary T , Dolan Jordyn , Harris Valerie Κ , Mellon Walker , Rupp Shawn M , Duke Elizabeth G , Harrison Tara M , Aksoy Selin , Giraudeau Mathieu , Vincze Orsolya , McGraw Kevin J , Aktipis Athena , Tollis Marc , Boddy Amy Μ , Maley Carlo C
Published: June 2024

Background and objectives

Cancer is a disease that affects nearly all multicellular life, including the broad and diverse taxa of Aves. While little is known about the factors that contribute to cancer risk across Aves, life history trade-offs may explain some of this variability in cancer prevalence. We predict birds with high investment in reproduction may have a higher likelihood of developing cancer. In this study, we tested whether life history traits are associated with cancer prevalence in 108 species of birds.

Methodology

We obtained life history data from published databases and cancer data from 5,729 necropsies from 108 species of birds across 24 taxonomic orders from 25 different zoological facilities. We performed phylogenetically controlled regression analyses between adult body mass, lifespan, incubation length, clutch size, sexually dimorphic traits, and both neoplasia and malignancy prevalence. We also compared the neoplasia and malignancy prevalence of female and male birds.

Results

Providing support for a life history trade-off between somatic maintenance and reproduction, we found a positive relationship between clutch size and cancer prevalence across Aves. There was no significant association with body mass, lifespan, incubation length, sexual dimorphism, and cancer.

Conclusions and implications

Life history theory presents an important framework for understanding differences in cancer defenses across various species. These results suggest a trade-off between reproduction and somatic maintenance, where Aves with small clutch sizes get less cancer.
Changes to circulating tumor cells in the central vein during laparoscopic versus transanal endoscopic surgeries for rectal cancer: can surgical approach make a difference?
Authors: Chen Mian , Ye Fujin , Zheng Wenwen , Xiong Li , Liang Zhenxing , Liu Huashan , Zheng Xiaobin , Li Wenxin , Kang Liang , Huang Liang
Published: June 2024

Background

The oncological safety of transanal total mesorectal excision (taTME) remains uncertain, and its special surgical approach may contribute to tumor cell dissemination. Thus, we conducted a study to investigate the impact of surgical approach on circulating tumor cell (CTC) counts and phenotypes in rectal cancer.

Methods

This is a prospective randomized controlled study (ClinicalTrials: NCT05109130). The patients were randomized to either the taTME (n = 49) or laparoscopic TME (laTME) (n = 48) groups. Blood samples were collected from the central vein to measure CTC counts and phenotypes at three time points: preoperative (t1), immediately post-tumor removal (t2), and one week post-surgery (t3). The effect of surgical procedure on CTCs at each time point was analyzed, with the primary endpoint being the change in CTC counts from t1 to t3 for each surgical approach. This study adheres to Consolidated Standards of Reporting Trials Guidelines.

Results

The baseline clinicopathologic characteristics of the laTME and taTME groups were balanced. The change in CTC count from t1 to t3 was 1.81 ± 5.66 in the laTME group and 2.18 ± 5.53 in the taTME group. The taTME surgery was non-inferior to laTME in terms of changing CTC counts (mean difference [MD]: −0.371; 95% confidence interval [CI]: −2.626 to 1.883, upper-sided 95% CI of 1.883 < 2, non-inferiority boundary value). Compared with that at t1, the CTC count at t2 did not change significantly. However, higher CTC counts were detected at t3 than at t2 in the taTME (P = 0.032) and laTME (P = 0.003) groups. From t1 to t3, CTC counts significantly increased in both the taTME (P = 0.008) and laTME (P = 0.031) groups. There were no significant differences in CTC phenotype changes between the two groups from t1 to t3.

Conclusions

Compared with laTME, taTME did not affect CTC counts and phenotypes. Our findings indicate that taTME is not inferior to laTME in terms of CTC changes from an oncological perspective.
DNA Transposons Favor De Novo Transcript Emergence Through Enrichment of Transcription Factor Binding Motifs
Authors: Lebherz Marie Kristin , Fouks Bertrand , Schmidt Julian , Bornberg-Bauer Erich , Grandchamp Anna
Published: June 2024

De novo genes emerge from noncoding regions of genomes via succession of mutations. Among others, such mutations activate transcription and create a new open reading frame (ORF). Although the mechanisms underlying ORF emergence are well documented, relatively little is known about the mechanisms enabling new transcription events. Yet, in many species a continuum between absent and very prominent transcription has been reported for essentially all regions of the genome. In this study, we searched for de novo transcripts by using newly assembled genomes and transcriptomes of seven inbred lines of Drosophila melanogaster, originating from six European and one African population. This setup allowed us to detect sample specific de novo transcripts, and compare them to their homologous nontranscribed regions in other samples, as well as genic and intergenic control sequences. We studied the association with transposable elements (TEs) and the enrichment of transcription factor motifs upstream of de novo emerged transcripts and compared them with regulatory elements. We found that de novo transcripts overlap with TEs more often than expected by chance. The emergence of new transcripts correlates with regions of high guanine-cytosine content and TE expression. Moreover, upstream regions of de novo transcripts are highly enriched with regulatory motifs. Such motifs are more enriched in new transcripts overlapping with TEs, particularly DNA TEs, and are more conserved upstream de novo transcripts than upstream their ‘nontranscribed homologs’. Overall, our study demonstrates that TE insertion is important for transcript emergence, partly by introducing new regulatory motifs from DNA TE families.
Long-Read–Based Hybrid Genome Assembly and Annotation of Snow Algal Strain CCCryo 101-99 (cf. Sphaerocystis sp., Chlamydomonadales)
Authors: Çiftçi Ozan , Zervas Athanasios , Lutz Stefanie , Feord Helen , Keusching Christoph , Leya Thomas , Tranter Martyn , Anesio Alexandre M , Benning Liane G
Published: June 2024

Polar regions harbor a diversity of cold-adapted (cryophilic) algae, which can be categorized into psychrophilic (obligate cryophilic) and cryotrophic (nonobligate cryophilic) snow algae. Both can accumulate significant biomasses on glacier and snow habitats and play major roles in global climate dynamics. Despite their significance, genomic studies on these organisms remain scarce, hindering our understanding of their evolutionary history and adaptive mechanisms in the face of climate change. Here, we present the draft genome assembly and annotation of the psychrophilic snow algal strain CCCryo 101-99 (cf. Sphaerocystis sp.). The draft haploid genome assembly is 122.5 Mb in length and is represented by 664 contigs with an N50 of 0.86 Mb, a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 92.9% (n = 1,519), a maximum contig length of 5.3 Mb, and a guanine-cystosine (GC) content of 53.1%. In total, 28.98% of the genome (35.5 Mb) contains repetitive elements. We identified 417 noncoding RNAs and annotated the chloroplast genome. The predicted proteome comprises 14,805 genes with a BUSCO completeness of 97.8%. Our preliminary analyses reveal a genome with a higher repeat content compared with mesophilic chlorophyte relatives, alongside enrichment in gene families associated with photosynthesis and flagella functions. Our current data will facilitate future comparative studies, improving our understanding of the likely response of polar algae to a warming climate as well as their evolutionary trajectories in permanently cold environments.
Misinformation surrounding sodium reduction for blood pressure: content analysis of Japanese posts on X
Authors: Terada Marina , Okuhara Tsuyoshi , Nishiie Yuriko , Kiuchi Takahiro , Murakami Kentaro
Published: June 2024

This content analysis aimed to assess misinformation themes regarding sodium reduction and blood pressure on X with the goal of providing strategies to address and debunk such misinformation. A total of 531 posts were manually coded into sodium-related misinformation themes, with inclusion criteria for posts asserting no association between sodium reduction and hypertension or claiming consuming sodium is beneficial for health. Numbers and post frequencies per misinformation theme were calculated. Post characteristics, including information sources, advertisements and narratives, were coded, and a correlation analysis was conducted to assess their association with each misinformation theme. Fourteen sodium-related misinformation themes were identified and consistently disseminated on X. The predominant theme, ‘Natural Salt’, accounted for 37.7% (n = 200), reaching 1.6 million followers, followed by ‘Reducing salt could be bad for my health’ theme, comprising 28.6% (n = 152) and reaching 1.5 million followers. There was a statistical correlation between the natural salt misinformation theme and advertisements. Many of the most frequent misinformation themes identified in this study have not been systematically debunked by organizations such as the World Health Organization and the American Heart Association. This study underscores the importance of continuous monitoring and analysis of sodium-related misinformation on social media platforms and their underlying commercial interests. Such monitoring has the potential to identify prevalent misinformation themes that may pose harm to the public and to inform public health organizations, enabling them to proactively address potential issues through debunking.
IBD Is Like a Tree: Reflections From Older Adults With Inflammatory Bowel Disease
Authors: Rusher Alison , Araka Elizabeth , Ananthakrishnan Ashwin N , Ritchie Christine , Kochar Bharati
Published: June 2024

Background

Despite the growing proportion of older adults with inflammatory bowel disease (IBD), their lived experience is not well understood. IBD literature is generally focused on younger adults, and few studies are qualitative. Older adults may report well-being differently than younger adults, so it is important that we learn about their goals and priorities with a chronic disease.

Objective

The study sought to understand the lived experience of older adults with IBD and explore their perceptions and priorities.

Methods

We conducted in-depth interviews with patients ≥60 years of age with IBD to evaluate the impact and perception of IBD in the context their overall health and life. We used a hybrid inductive-deductive thematic analysis of our transcripts to identify underlying patterns.

Results

We achieved thematic saturation after 22 interviews. We produced 4 major themes: (1) having IBD at an older age, (2) financial ramifications of IBD at an older age, (3) expectations for a meaningful life, and (4) unmet needs. Prominent subthemes included (1) ageism, loss of autonomy, and barriers to healthcare; (2) retirement and insurance issues; (3) redefining quality of life and gratitude; and (4) social isolation and navigating daily life with IBD.

Conclusions

Having IBD later in life presents unique challenges. Physicians treating older patients should consider age-sensitive communication, susceptibility to social isolation, and practices for healthy aging in the context of IBD. Patient priorities for further investigation include more representation in the media and educational material tailored for older adults with IBD.
When to Go Against the Flow: Examining Patterns of Performance Over Multiday Migration Events in the Hawaiian Stream Fish, ‘O‘opu Nōpili (Sicyopterus stimpsoni)
Authors: Diamond Kelly M , Nishiura Lance , Sakihara Troy , Schoenfuss Heiko L , Blob Richard W
Published: June 2024

Synopsis

Many animals migrate across regions of their geographic range as part of extended events, with groups of individuals proceeding through areas of travel on several successive days. Early migrating individuals may have an advantage over late migrating individuals by gaining early access to the resources at the eventual destination. For situations where early access to resources would provide an advantage, specific sets of locomotor traits might be found among individuals that are earlier migrators. We tested for associations between migration timing and traits related to escape responses, climbing, and morphology in the amphidromous Hawaiian stream goby, ‘o‘opu nōpili (Sicyopterus stimpsoni). In this species, juvenile fish migrate in pulses over several days immediately following flash floods. We collected daily measurements of escape responses and waterfall climbing from juvenile fish arriving at streams from the ocean. We found that escape performance showed mainly stochastic variation across migrating individuals tested on successive days. In contrast, some metrics of climbing performance decrease over successive pulses during a migration event. We also found more variation in body shape among fish from early pulses during migration events compared to later in pulses. These results could have implications for guiding conservation efforts, identifying critical time windows for protection as periods with the greatest likelihood of successful migrants.
Less invasive replacement of aortic root, ascending aorta and hemiarch via partial upper sternotomy: a propensity-score-matched comparison with full sternotomy
Authors: Papadopoulos Nestoras , Ntinopoulos Vasileios , Haeussler Achim , Odavic Dragan , Risteski Petar , Biefer Héctor Rodríguez Cetina , Dzemali Omer
Published: June 2024

OBJECTIVES

Less invasive surgery has emerged as an option for aortic pathologies. The current study compared our experience on early postoperative results of patients with aortic surgery between partial upper sternotomy (PUS) and full sternotomy (FS).

METHODS

We performed a retrospective analysis of the data of patients undergoing aortic root surgery with concomitant ascending aorta and hemiarch replacement. Exclusion criteria were type A aortic dissection and other concomitant major cardiac surgery. After propensity score matching, we compared the perioperative outcomes of patients undergoing surgery with PUS versus FS.

RESULTS

A total of 161 patients operated on between January 2013 and September 2022 met the inclusion criteria (PUS: n = 22, FS: n = 139). Propensity score matching yielded 22 pairs with a balanced distribution of propensity scores and covariates between the compared groups. There was no evidence that PUS affects cardiopulmonary bypass [108 (67–119) vs 113 (87–148) min, P = 0.154; PUS vs FS] and circulatory arrest duration [9 (7–10) vs 9 (8–13) min, P = 0.264; PUS vs FS]. There was a reduced cross-clamp duration in the PUS group [88 (58–96) vs 92 (71–122) min, P = 0.032]. Cumulative sum charts have shown consistently low cross-clamp and circulatory arrest duration for 2 experienced surgeons who performed 20 of the procedures in the PUS group (10 each). Perioperative mortality and morbidity were low, with no in-hospital mortality in the PUS group [0 vs 1(4.5%), P > 0.999] and absence of strokes in both groups.

CONCLUSIONS

In summary, our initial experience suggests that less invasive aortic root, ascending aorta and hemiarch replacement via PUS could be performed in our patient cohort as safely as via full sternotomy. Advantages for the patient are reduced surgical trauma, improved cosmetic results and—presumably—less pain.
Russia's Leadership in Eurasia: Holding Together or Falling Apart?
Authors: Roberts Sean , Ziemer Ulrike
Published: June 2024

Abstracts

The Russo–Ukraine War raises important questions on the dynamics of regional leadership and followership in what may be termed “Russian-led Eurasia.” These questions, in particular, the strength of Russian leadership in the region is complicated by the ambiguity in existing literature and competing images of Russia's relations with long-standing allies—notably Armenia, Belarus, Kazakhstan and Kyrgyzstan—which are often portrayed in terms of a “community of fate” or partners destined for closer integration but also as a “community of fortune” or ad hoc, situational partners, loosely centered on Russia. This article offers an innovative theoretical and methodological exploration of Russia's relations with regional partner states by utilizing the English School of International Relations and regional integration organizations to assess Russia's regional leadership. As argued in this article, Russian-led Eurasia may be understood as an example of a regional interstate society with Russian hegemony serving as a socially conferred, binding institution. But this hegemony is inherently unstable owing to Russia's inability to balance hegemonic “rights” with “responsibilities.” War in Ukraine did not create this problem, but it has created the conditions for leadership transition in the region.
Isavuconazole as prophylaxis and therapy for invasive fungal diseases: a real-life observational study
Authors: Ergün Mehmet , Jansen Anouk M E , Hilbrands Luuk B , de Kort Elizabeth , Kunst Henricus , Reijers Monique H E , Schouten Jeroen A , Verweij Paul E , Brüggemann Roger J M
Published: June 2024

Background

Isavuconazole is a relatively new antifungal agent indicated for the management of various invasive fungal diseases (IFDs), including invasive aspergillosis. Information on real-world experience with isavuconazole is scarce. This retrospective observational study aimed to describe the usage of isavuconazole in clinical practice with an in-depth evaluation of individual isavuconazole exposure.

Methods

Patients treated with isavuconazole were evaluated based on retrospective data, including therapeutic drug monitoring (TDM) data and efficacy and safety data. Additionally, we calculated the individual isavuconazole exposure described by the average AUC24 over the first 7 days of treatment by means of non-linear mixed-effects modelling and compared this with the currently desired lower target AUC of 60 mg·h/L.

Results

Ninety-nine patients treated with isavuconazole were evaluated. In our real-life cohort, isavuconazole was often deployed off-label in patients with non-classical host factors and infections with non-Aspergillus and non-Mucorales species. Isavuconazole was most often chosen for its safety profile, even after prior triazole treatment with manifestations of toxicity. TDM and subsequent dosage adjustments were frequently performed. The individual average AUC24 over 7 days was above 60 mg·h/L in 29 out of 77 (37.7%) patients.

Conclusions

This overview provides practical insights that can aid clinicians in the management of their patients with IFD. Our study shows that isavuconazole was used in a diverse patient population and was well tolerated overall. Individual isavuconazole exposure reflected by the average AUC24 over the first 7 days of treatment was generally low and variable. Dosage adjustments following TDM were frequently performed. Our experience shows that isavuconazole is a feasible alternative after prior azole treatment.
Stratifying heart failure patients with graph neural network and transformer using Electronic Health Records to optimize drug response prediction
Authors: Chowdhury Shaika , Chen Yongbin , Li Pengyang , Rajaganapathy Sivaraman , Wen Andrew , Ma Xiao , Dai Qiying , Yu Yue , Fu Sunyang , Jiang Xiaoqian , He Zhe , Sohn Sunghwan , Liu Xiaoke , Bielinski Suzette J , Chamberlain Alanna M , Cerhan James R , Zong Nansu
Published: June 2024

Objectives

Heart failure (HF) impacts millions of patients worldwide, yet the variability in treatment responses remains a major challenge for healthcare professionals. The current treatment strategies, largely derived from population based evidence, often fail to consider the unique characteristics of individual patients, resulting in suboptimal outcomes. This study aims to develop computational models that are patient-specific in predicting treatment outcomes, by utilizing a large Electronic Health Records (EHR) database. The goal is to improve drug response predictions by identifying specific HF patient subgroups that are likely to benefit from existing HF medications.

Materials and Methods

A novel, graph-based model capable of predicting treatment responses, combining Graph Neural Network and Transformer was developed. This method differs from conventional approaches by transforming a patient's EHR data into a graph structure. By defining patient subgroups based on this representation via K-Means Clustering, we were able to enhance the performance of drug response predictions.

Results

Leveraging EHR data from 11 627 Mayo Clinic HF patients, our model significantly outperformed traditional models in predicting drug response using NT-proBNP as a HF biomarker across five medication categories (best RMSE of 0.0043). Four distinct patient subgroups were identified with differential characteristics and outcomes, demonstrating superior predictive capabilities over existing HF subtypes (best mean RMSE of 0.0032).

Discussion

These results highlight the power of graph-based modeling of EHR in improving HF treatment strategies. The stratification of patients sheds light on particular patient segments that could benefit more significantly from tailored response predictions.

Conclusions

Longitudinal EHR data have the potential to enhance personalized prognostic predictions through the application of graph-based AI techniques.
Automatic uncovering of patient primary concerns in portal messages using a fusion framework of pretrained language models
Authors: Ren Yang , Wu Yuqi , Fan Jungwei W , Khurana Aditya , Fu Sunyang , Wu Dezhi , Liu Hongfang , Huang Ming
Published: June 2024

Objectives

The surge in patient portal messages (PPMs) with increasing needs and workloads for efficient PPM triage in healthcare settings has spurred the exploration of AI-driven solutions to streamline the healthcare workflow processes, ensuring timely responses to patients to satisfy their healthcare needs. However, there has been less focus on isolating and understanding patient primary concerns in PPMs—a practice which holds the potential to yield more nuanced insights and enhances the quality of healthcare delivery and patient-centered care.

Materials and Methods

We propose a fusion framework to leverage pretrained language models (LMs) with different language advantages via a Convolution Neural Network for precise identification of patient primary concerns via multi-class classification. We examined 3 traditional machine learning models, 9 BERT-based language models, 6 fusion models, and 2 ensemble models.

Results

The outcomes of our experimentation underscore the superior performance achieved by BERT-based models in comparison to traditional machine learning models. Remarkably, our fusion model emerges as the top-performing solution, delivering a notably improved accuracy score of 77.67 ± 2.74% and an F1 score of 74.37 ± 3.70% in macro-average.

Discussion

This study highlights the feasibility and effectiveness of multi-class classification for patient primary concern detection and the proposed fusion framework for enhancing primary concern detection.

Conclusions

The use of multi-class classification enhanced by a fusion of multiple pretrained LMs not only improves the accuracy and efficiency of patient primary concern identification in PPMs but also aids in managing the rising volume of PPMs in healthcare, ensuring critical patient communications are addressed promptly and accurately.
Validation and Collaborative Studies of Headspace Gas Chromatography-Mass Spectrometry for Determination of 1,4-Dioxane in Cosmetic Samples
Authors: Lusianti Erita , Zannah Nenden Solihatul , Purwaningsih Sri , Kusuma Hasti , Hastuti Agustina A M B , Windarsih Anjar , Cheah Nuan Ping , Thongprakaisang Siriporn , Rohman Abdul
Published: June 2024

Background

1,4-Dioxane (1,4-D) is a byproduct of the synthesis of surfactants, typically found in some cosmetics products such as shampoo, toothpaste, and soap. The presence of 1,4-D in cosmetics products is limited to a certain amount since 1,4-D is classified as a probable human carcinogen.

Objective

This present study was intended to validate static headspace gas chromatography-mass spectrometry (HS GC-MS) for the determination of 1,4-D in cosmetics products.

Methods

The condition of HS and GC-MS was optimized to get the best condition for analysis of 1,4-D using 1,4-dioxane-d8 (1,4-D-d8) as internal standard (IS). The developed method was validated by evaluating the key performance characteristics, including specificity, linearity, limit of detection (LoD), limit of quantification (LoQ), accuracy, and precision.

Results

The results showed that HS GC-MS was specific since the peaks of the selected ion monitoring (SIM) mode could be separated and confirmed at m/z 88 and m/z 96 for 1,4-D and 1,4-D-d8, respectively. The method was linear over the concentration range of 0.1287–1.2875 µg/mL, with R2 > 0.999 and RSD residuals <2.0. A collaborative study was conducted on this method, with 10 participating laboratories from four countries. The outcome of this study was found to be accurate and precise, as evidenced by the excellent recoveries ranging from 94.6% to 102.1%, and with good reproducibility with RSD values ranging from 0.2 to 1.1%. The collaborative studies exhibited that all data reported by 10 participating laboratories in four countries were inliers without any extreme values observed either in mean or RSD values

Conclusions

HS GC-MS is found to be fit and suitable for the determination of trace level of 1,4-D in cosmetics products.

Highlights

The HS GC-MS method could be proposed as a standard method for quantitative analysis of 1,4-D in cosmetics products since the collaborative studies indicated that the developed method meet the requirement in “Guidelines for Collaborative Study Procedures to Validate Characteristics of a Method of Analysis.”
First report of resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to lambda-cyhalothrin from Pakistan: baseline susceptibility, selection, occurrence of cross-resistance, realized heritability, and inheritance mode of resistance
Authors: Zaidi Sayed Wasif Naseer , Saddiq Bushra , Afzal Muhammad Babar Shahzad , Banazeer Ansa , Serrão José Eduardo , Farooq Umar , Baloch Muhammad Affan Zahid
Published: June 2024

Fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a global pest causing damage to several crops. However, its management using chemical control is a challenge due to its capacity to evolve resistance to insecticides. After 6 generations of selection with lambda-cyhalothrin, the LC50 for the insecticide-resistant strain (Lambda-Sel) was 486 ppm, higher than that of the field strain (FAW-MUL) (7.5 ppm), susceptible laboratory strain (Lab-PK) (0.46 ppm), and laboratory unselected strain (UNSEL) (5.26 ppm). Laboratory selection with lambda-cyhalothrin increased resistance from 16.3- to 1056.52-fold and 1.43- to 92.4-fold to lambda-cyhalothrin compared to Lab-PK and UNSEL strains, respectively. The selected strain of S. frugiperda (Lambda-Sel) presented low cross-resistance to chlorpyrifos, moderate to deltamethrin and indoxacarb, very low to spinosad, and no cross-resistance to emamectin benzoate. The realized heritability (h2) of lambda-cyhalothrin resistance in the Lambda-Sel strain was very high (0.88). The reciprocal cross progenies of F1 (Lambda-Sel ♀ × Lab-PK ♂), F1ʹ (Lambda-Sel ♂ × Lab-PK ♀), BC1 (F1 ♀ × Lambda-Sel ♂), and BC2 (F1 ♀ × Lab-PK ♂) showed high resistance ratios of 545.64-, 396.52-, 181.18-, and 146.54-fold, respectively compared to Lab-PK. The degree of dominance values for lambda-cyhalothrin in F1 and F1ʹ indicates incompletely dominant resistance. The difference between observed and expected mortality in backcross populations (BC1 and BC2) revealed a polygenic resistance. In conclusion, the resistance to lambda-cyhalothrin was autosomal, incompletely dominant, and polygenic. These findings provide new insights for insect resistance management strategies to mitigate the occurrence of resistance in this global pest.
Novel transinfections of Rickettsiella do not affect insecticide tolerance in Myzus persicae, Rhopalosiphum padi, or Diuraphis noxia (Hemiptera: Aphididae)
Authors: Dorai Ashritha Prithiv Sivaji , Umina Paul A , Chirgwin Evatt , Yang Qiong , Gu Xinyue , Thia Joshua , Hoffmann Ary
Published: June 2024

Aphids (Hemiptera: Aphidoidea) are economically important crop pests worldwide. Because of growing issues with insecticide resistance and environmental contamination by insecticides, alternate methods are being explored to provide aphid control. Aphids contain endosymbiotic bacteria that affect host fitness and could be targeted as potential biocontrol agents, but such novel strategies should not impact the effectiveness of traditional chemical control. In this work, we used a novel endosymbiont transinfection to examine the impact of the endosymbiont Rickettsiella viridis on chemical tolerance in 3 important agricultural pest species of aphid: Myzus persicae (Sulzer) (Hemiptera: Aphididae), Rhopalosiphum padi (Linnaeus) (Hemiptera: Aphididae), and Diuraphis noxia (Mordvilko ex Kurdjumov) (Hemiptera: Aphididae). We tested tolerance to the commonly used insecticides alpha-cypermethrin, bifenthrin, and pirimicarb using a leaf-dip bioassay. We found no observed effect of this novel endosymbiont transinfection on chemical tolerance, suggesting that the strain of Rickettsiella tested here could be used as a biocontrol agent without affecting sensitivity to insecticides. This may allow Rickettsiella transinfections to be used in combination with chemical applications for pest control. The impacts of other endosymbionts on insecticide tolerance should be considered, along with tests on multiple aphid clones with different inherent levels of chemical tolerance.
Eliminating explanations for Maladera formosae (Coleoptera: Scarabaeidae) preponderance in sandy soil
Authors: Pekarcik Adrian J , Ranger Christopher M , Long Elizabeth Y , Tilmon Kelley J
Published: June 2024

Most field corn in the United States receives a neonicotinoid seed treatment for the management of early-season, soil-dwelling insect pests. Grubs of Maladera formosae (Brenske) (Coleoptera: Scarabaeidae) have been reported feeding on young field corn with both low and high rates of clothianidin seed treatments in Indiana, Michigan, and Ohio. Anecdotally, these infestations are restricted to sandy soils in the region. The purpose of this study was to (1) evaluate whether grub populations in corn are restricted to sandy soils, (2) assess whether soil type influences M. formosae survival, and (3) determine whether soil type affects clothianidin uptake by the plant, possibly explaining the observed differences in M. formosae abundance by soil type. We observed nearly 10-times more grubs in sand (>80% sand content) than loam (<80% sand content) soil within a single corn field. Grub survival to adult was not influenced by soil type. We then compared the concentrations of clothianidin seed treatment in the roots and shoots of corn seedlings grown in either sand or loam soil over time. Similar amounts of the active ingredient were found in the roots and shoots of corn grown in both soil types. Within 2 week, the clothianidin concentrations in both soil types had significantly declined in roots and shoots and were no different from the no-insecticide control. These findings suggest that factors other than insecticide exposure contribute to the higher abundance of M. formosae larvae in sand relative to loam soils, even within the same field.
A correspondence theorem for interpretability logic with respect to Verbrugge semantics
Authors: Horvat Sebastijan , Perkov Tin
Published: June 2024

Interpretability logic is a modal logic that can be used to describe relative interpretability between extensions of a given first-order arithmetical theory. Verbrugge semantics is a generalization of the basic semantics for interpretability logic. Bisimulation is the basic equivalence between models for modal logic. The Van Benthem Correspondence Theorem establishes modal logic as the bisimulation invariant fragment of first-order logic. In this paper we show that a special type of bisimulations, the so-called w-bisimulations, enable an analogue of the Van Benthem Theorem for interpretability logic w.r.t. Verbrugge semantics.
A review of molt in mammals, with an emphasis on marmots (Rodentia: Sciuridae: Marmota)
Authors: Mills Kendall K , Brandler Oleg V , Olson Link E
Published: June 2024

Molting is an evolutionarily ancient trait in which the outermost layer of an organism is replenished, usually according to a regular circannual rhythm. It is a metabolically costly process and, in vertebrates, is generally timed around other energetically demanding events such as reproduction and migration. In mammals, molting involves replacement of the fur coat—one of the most distinct innovations of the mammalian lineage. Despite the obvious importance of hair to mammalian fitness, our knowledge of hair growth cycles, circannual molting patterns, and hair structure remains largely restricted to marine and domesticated mammals, and our ability to identify explicit adaptive advantages of molting strategies in any mammal is therefore limited. In this review, we summarize what is known of these topics in wild, terrestrial mammals with a particular emphasis on marmots (Marmota spp.). Marmots are the largest extant ground squirrels and are well adapted to seasonally cold environments. Molting may be particularly relevant to fitness in marmots given the presumed importance of a healthy, insulative coat for metabolic efficiency in cold environments. Moreover, marmots hibernate for 7 to 8 months each year, meaning the annual molt and all other energetically demanding life-history events (such as parturition, lactation, fat accumulation, and dispersal) are constrained to an active period of only 4 to 5 months. Because the energetics of hibernation, fat accumulation, reproduction, and social behavior are already well studied, examining how molt is timed with respect to other important events and how it is influenced by local conditions may inform how molting is prioritized and how molting strategies evolve under specific selective pressures.
Therapeutic effects of Tanshinone IIA and Tetramethylpyrazine nanoemulsions on cognitive impairment and neuronal damage in Alzheimer’s disease rat models
Authors: Fang Liang , Cheng Hongyan , Chen Weidong , Peng Can , Liu Yuanxu , Zhang Caiyun
Published: June 2024

Objectives

The aim of this study was to investigate the therapeutic effects and related mechanisms of Tanshinone IIA and Tetramethylpyrazine O/W composite nanoemulsions on Alzheimer’s disease (AD) rats.

Methods

The therapeutic effect of TSN/TMP O/W NEs on AD rats was evaluated by behavioral tests, H&E, Nissl, and Immunohistochemistry staining. ELISA and Western blot were used to analyze the mechanism.

Key findings

The results showed that TSN/TMP O/W NEs could down-regulate the expression of Bax and Caspase-3 proteins, decrease the level of MDA, increase the expression of SOD and GSH-Px, and alleviate cognitive impairment in AD rats.

Conclusions

TSN/TMP O/W NEs can inhibit MAPK/ERK/CREB signaling pathway and effectively alleviate cognitive impairment, oxidative stress injury, and neuronal apoptosis in AD rats.
Why you should avoid using multiple Fine–Gray models: insights from (attempts at) simulating proportional subdistribution hazards data
Authors: Bonneville Edouard F , de Wreede Liesbeth C , Putter Hein
Published: June 2024

Studies considering competing risks will often aim to estimate the cumulative incidence functions conditional on an individual’s baseline characteristics. While the Fine–Gray subdistribution hazard model is tailor-made for analysing only one of the competing events, it may still be used in settings where multiple competing events are of scientific interest, where it is specified for each cause in turn. In this work, we provide an overview of data-generating mechanisms where proportional subdistribution hazards hold for at least one cause. We use these to motivate why the use of multiple Fine–Gray models should be avoided in favour of better alternatives such as cause-specific hazard models.
Capitalizing on genebank core collections for rare and novel disease resistance loci to enhance barley resilience
Authors: Yuan Zhihui , Rembe Maximilian , Mascher Martin , Stein Nils , Jayakodi Murukarthick , Börner Andreas , Oldach Klaus , Jahoor Ahmed , Jensen Jens Due , Rudloff Julia , Dohrendorf Viktoria-Elisabeth , Kuhfus Luisa Pauline , Dyrszka Emmanuelle , Conte Matthieu , Hinz Frederik , Trouchaud Salim , Reif Jochen C , El Hanafi Samira
Published: June 2024

In the realm of agricultural sustainability, the utilization of plant genetic resources for enhanced disease resistance is paramount. Preservation efforts in genebanks are justified by their potential contributions to future crop improvement. To capitalize on the potential of plant genetic resources, we focused on a barley core collection from the German ex situ genebank and contrasted it with a European elite collection. The phenotypic assessment included 812 plant genetic resources and 298 elites, with a particular emphasis on four disease traits (Puccinia hordei, Blumeria graminis hordei, Ramularia collo-cygni, and Rhynchosporium commune). An integrated genome-wide association study, employing both Bayesian-information and linkage-disequilibrium iteratively nested keyway (BLINK) and a linear mixed model, was performed to unravel the genetic underpinnings of disease resistance. A total of 932 marker–trait associations were identified and assigned to 49 quantitative trait loci. The accumulation of novel and rare resistance alleles significantly bolstered the overall resistance level in plant genetic resources. Three plant genetic resources donors with high counts of novel/rare alleles and exhibiting exceptional resistance to leaf rust and powdery mildew were identified, offering promise for targeted pre-breeding goals and enhanced resilience in future varieties. Our findings underscore the critical contribution of plant genetic resources to strengthening crop resilience and advancing sustainable agricultural practices.
Oral microbiota in aging and diseases
Authors: Ren Ya , Chen Mingxu , Wang Ziyang , Han Jing-Dong J
Published: June 2024

Human microbiomes are microbial populations that form a symbiotic relationship with humans. There are up to 1000 species on the surface of human skin and mucosal system, among which gut microbiota attracts the most interest. As the beginning of the digestive tract, oral cavity is also an important microbial habitat in the human body which is the first line of defense against pathogens entering the body. Many studies have revealed that oral microbial dysbiosis could not only contribute to oral diseases but also whole-body systemic diseases and health status. Oral microorganisms can enter the gastrointestinal tract with saliva and food, or enter the blood circulation through mouth breakage, thus causing systemic inflammation and aging-related diseases including some causal links to Alzheimer’s disease. A series of changes take place in oral microbial composition during development, with different age stages marked by different dominant microbial species. Despite a lack of comprehensive studies on aging oral microbiota, through systemic inflammation, oral pathogenic microbes are likely to contribute inflammatory aging. As inflammaging is a key signature and one of the causes for accelerated aging, improving the structure of oral microbiome may be not only a new strategy for disease prevention and treatment, but also for aging intervention.
Functional Outcomes Following Internal Fixation versus Hemiarthroplasty for Displaced Intracapsular Neck of Femur Fractures
Authors: Naqvi Ali Z. , Pearse Michael
Published: June 2024

Hip fractures are a common injury requiring input from orthopaedic surgeons throughout the world. Surgical management is important in reducing the risk of disability, increased dependence, and morbidity and mortality. The method of surgical intervention has been guided by surgeon choice and patient factors, and the question that arises is whether to “fix or replace.” This chapter will analyze a prospective randomized controlled trial comparing functional outcomes following two surgical options: internal fixation vs. hemiarthroplasty with a two-year follow-up. With validated functional outcome scores being used as primary outcome measures, this chapter will explore which of these interventions results in improved patient-reported functional outcomes and therefore decreased disability following this life-changing injury.
Highly Cross-Linked Polyethylene Reduces Wear and Revision Rates in Total Hip Arthroplasty
Authors: Monem Mohammed , Sugand Kapil , Pearse Michael
Published: June 2024

Aseptic loosening is the most common reason for revision of total hip replacements (THRs). Understanding which type of liners to use to reduce debris-related osteolysis and loosening is important and should lead to better clinical outcomes. This double-blinded study compares the clinical and radiological outcomes of two different liners, Marathon highly cross-linked polyethylene (XLPE) vs. Enduron ultra-high-molecular-weight polyethylene (UHMWPE), in THRs followed up for at least a decade. The stem and acetabular shells used in the study were the same design and from the same manufacturer. The results of this study will help to inform the surgeon’s decision-making in choosing their acetabular liners for both primary and revision THRs.