bluuebunny/crossref_metadata_2025 huggingface dataset:
Stem Cell Therapy against Ischemic Heart Disease
Authors: Tsai I-Ting , Sun Cheuk-Kwan
Published: March 2024

Ischemic heart disease, which is one of the top killers worldwide, encompasses a series of heart problems stemming from a compromised coronary blood supply to the myocardium. The severity of the disease ranges from an unstable manifestation of ischemic symptoms, such as unstable angina, to myocardial death, that is, the immediate life-threatening condition of myocardial infarction. Even though patients may survive myocardial infarction, the resulting ischemia-reperfusion injury triggers a cascade of inflammatory reactions and oxidative stress that poses a significant threat to myocardial function following successful revascularization. Moreover, despite evidence suggesting the presence of cardiac stem cells, the fact that cardiomyocytes are terminally differentiated and cannot significantly regenerate after injury accounts for the subsequent progression to ischemic cardiomyopathy and ischemic heart failure, despite the current advancements in cardiac medicine. In the last two decades, researchers have realized the possibility of utilizing stem cell plasticity for therapeutic purposes. Indeed, stem cells of different origin, such as bone-marrow- and adipose-derived mesenchymal stem cells, circulation-derived progenitor cells, and induced pluripotent stem cells, have all been shown to play therapeutic roles in ischemic heart disease. In addition, the discovery of stem-cell-associated paracrine effects has triggered intense investigations into the actions of exosomes. Notwithstanding the seemingly promising outcomes from both experimental and clinical studies regarding the therapeutic use of stem cells against ischemic heart disease, positive results from fraud or false data interpretation need to be taken into consideration. The current review is aimed at overviewing the therapeutic application of stem cells in different categories of ischemic heart disease, including relevant experimental and clinical outcomes, as well as the proposed mechanisms underpinning such observations.
MicroRNA Expression Profiles in Human Samples and Cell Lines Revealed Nine miRNAs Associated with Cisplatin Resistance in High-Grade Serous Ovarian Cancer
Authors: Flores-Colón Marienid , Rivera-Serrano Mariela , Reyes-Burgos Víctor G. , Rolón José G. , Pérez-Santiago Josué , Marcos-Martínez María J. , Valiyeva Fatima , Vivas-Mejía Pablo E.
Published: March 2024

Metastasis and drug resistance are major contributors to cancer-related fatalities worldwide. In ovarian cancer (OC), a staggering 70% develop resistance to the front-line therapy, cisplatin. Despite proposed mechanisms, the molecular events driving cisplatin resistance remain unclear. Dysregulated microRNAs (miRNAs) play a role in OC initiation, progression, and chemoresistance, yet few studies have compared miRNA expression in OC samples and cell lines. This study aimed to identify key miRNAs involved in the cisplatin resistance of high-grade-serous-ovarian-cancer (HGSOC), the most common gynecological malignancy. MiRNA expression profiles were conducted on RNA isolated from formalin-fixed-paraffin-embedded human ovarian tumor samples and HGSOC cell lines. Nine miRNAs were identified in both sample types. Targeting these with oligonucleotide miRNA inhibitors (OMIs) reduced proliferation by more than 50% for miR-203a, miR-96-5p, miR-10a-5p, miR-141-3p, miR-200c-3p, miR-182-5p, miR-183-5p, and miR-1206. OMIs significantly reduced migration for miR-183-5p, miR-203a, miR-296-5p, and miR-1206. Molecular pathway analysis revealed that the nine miRNAs regulate pathways associated with proliferation, invasion, and chemoresistance through PTEN, ZEB1, FOXO1, and SNAI2. High expression of miR-1206, miR-10a-5p, miR-141-3p, and miR-96-5p correlated with poor prognosis in OC patients according to the KM plotter database. These nine miRNAs could be used as targets for therapy and as markers of cisplatin response.
Erythrocyte Glutathione S-Transferase Activity as a Sensitive Marker of Kidney Function Impairment in Children with IgA Vasculitis
Authors: Frkovic Marijan , Turcic Ana , Gagro Alenka , Srsen Sasa , Frkovic Sanda Huljev , Rogic Dunja , Jelusic Marija
Published: March 2024

IgA vasculitis (IgAV) is the most common childhood vasculitis. The main cause of morbidity and mortality in children with IgAV is nephritis (IgAVN), but the risk of its development, severity, and chronicity remain unclear. Erythrocyte glutathione S-transferase (e-GST) activity has been previously detected as a sensitive marker of kidney function impairment in several diseases. We spectrophotometrically assessed and correlated e-GST activity between 55 IgAV patients without nephritis (IgAVwN), 42 IgAVN patients, and 52 healthy controls. At disease onset, e-GST activity was significantly higher in IgAVN patients (median (interquartile range)) (5.7 U/gHb (4.4–7.5)) than in IgAVwN patients (3.1 U/gHb (2.2–4.2); p < 0.001), and controls (3.1 U/gHb (1.9–4.2); p < 0.001). Therewithal, there were no differences between the IgAVwN patients and controls (p = 0.837). e-GST activity was also significantly higher in the IgAVN patients than in the IgAVwN patients after 3 months (5.0 U/gHb (4.2–6.2) vs. 3.3 U/gHb (2.3–4.1); p < 0.001) and 6 months (4.2 U/gHb (3.2–5.8) vs. 3.3 U/gHb (2.1–4.1); p < 0.001) since the disease onset. Consistent correlations between e-GST activity and serum creatinine, estimated glomerular filtration rate (eGFR), and proteinuria levels were not detected. In conclusion, increased e-GST activity can serve as a subtle indicator of kidney function impairment in children with IgAV.
Research Progress on Bioactive Factors against Skin Aging
Authors: He Xin , Gao Xinyu , Guo Yifan , Xie Weidong
Published: March 2024

The relentless pursuit of effective strategies against skin aging has led to significant interest in the role of bioactive factors, particularly secondary metabolites from natural sources. The purpose of this study is to meticulously explore and summarize the recent advancements in understanding and utilization of bioactive factors against skin aging, with a focus on their sources, mechanisms of action, and therapeutic potential. Skin, the largest organ of the body, directly interacts with the external environment, making it susceptible to aging influenced by factors such as UV radiation, pollution, and oxidative stress. Among various interventions, bioactive factors, including peptides, amino acids, and secondary metabolites, have shown promising anti-aging effects by modulating the biological pathways associated with skin integrity and youthfulness. This article provides a comprehensive overview of these bioactive compounds, emphasizing collagen peptides, antioxidants, and herbal extracts, and discusses their effectiveness in promoting collagen synthesis, enhancing skin barrier function, and mitigating the visible signs of aging. By presenting a synthesis of the current research, this study aims to highlight the therapeutic potential of these bioactive factors in developing innovative anti-aging skin care solutions, thereby contributing to the broader field of dermatological research and offering new perspectives for future studies. Our findings underscore the importance of the continued exploration of bioactive compounds for their potential to revolutionize anti-aging skin care and improve skin health and aesthetics.
Comprehensive Empirical Modeling of Shear Strength Prediction in Reinforced Concrete Deep Beams
Authors: Sayhood Eyad K. , Mohammed Nisreen S. , Hilo Salam J. , Salih Salih S.
Published: March 2024

This paper presents comprehensive empirical equations to predict the shear strength capacity of reinforced concrete deep beams, with a focus on improving the accuracy of existing codes. Analyzing 198 deep beams imported from 15 existing investigations, this study considers various parameters such as concrete compressive strength (f′c), the shear span-to-effective depth ratio (av/d), and reinforcement ratios (ps, pv, and ph). Introducing a novel predictive empirical equation, this study conducts a rigorous evaluation using statistical metrics and a linear regression analysis (MAE, RMSE, and R2). The proposed model demonstrates a significant reduction in the coefficient of variation (CV) to 27.08%, compared to the existing codes’ limitations. Comparative analyses highlight the accuracy of the empirical equation, revealing an improved convergence of data points and minimal sensitivity to variations in key parameters. The results proved that the proposed empirical equation enhanced the accuracy to predict the shear strength capacity of the reinforced concrete deep beams in various scenarios, making it a valuable tool for structural engineers. This research contributes to advancing the understanding of shear strength capacity in reinforced concrete deep beams, offering a reliable empirical equation with implications for refining design methodologies and enhancing safety with the efficiency of structural systems.
Enhancement of Polyacrylic Acid/Silicon Carbide Nanocomposites’ Optical Properties for Potential Application in Renewable Energy
Authors: Rashid Farhan Lafta , Hashim Ahmed , Dulaimi Anmar , Hadi Aseel , Ibrahim Hamed , Al-Obaidi Mudhar A. , Ameen Arman
Published: March 2024

Composites made from polymers and nanoparticles have promise to be effective solar collectors and thermal energy storage devices due to benefits including improved thermal characteristics and increased structural stability. This study intends to fabricate polyacrylic acid/silicon carbide (PAA−SiC) nanocomposites and examine the optical properties for use in solar collectors and thermal energy storage (TES) fields. The optical properties of PAA−SiC nanocomposites are investigated within the wavelength between 340 and 840 nm. The findings indicate that an increase in SiC concentration in the PAA aqueous solution to 50 g/L at a wavelength of λ = 400 nm causes an increase in the absorption by 50.2% besides a reduction in transmission by 6%. Furthermore, the energy band gaps were reduced from 3.25 eV to 2.95 eV to allow for the transition, and subsequently reduced from 3.15 eV to 2.9 eV to allow for forbidden transition as a result of the increasing SiC concentration from 12.5 g/L to 50 g/L. The optical factors of energy absorption and optical conductivity were also enhanced with a rising SiC concentration from 12.5 to 50 g/L. Specifically, an improvement of 61% in the melting time of PAA−SiC−H2O nanofluids is concluded. Accordingly, it can be said that the PAA−SiC−H2O nanofluids are suitable for renewable energy and TES systems.
The Relationship between Posture and Muscle Tensive Dysphonia in Teachers: A Systematic Scoping Review
Authors: Franzone Roberta , Petrigna Luca , Signorelli Daniele , Musumeci Giuseppe
Published: March 2024

Teachers usually present work-related pain such as neck pain. Their posture could be the cause of these problems; indeed, it is often a sway-back posture. Furthermore, teachers can also experience problems with their voice such as dysphonia, specifically muscle tension dysphonia (MTD). This scoping review aims to find the correlation between teachers’ posture and MTD. It also studies how a posture-based treatment can influence this disorder. Randomized controlled trials, controlled clinical trials, prospective cohort studies, and cross-sectional studies that considered the relationship between posture and MTD and that included teachers in their sample. The search led to an initial number of 396 articles; after the screening process, a final number of eight articles were included. A total of 303 patients were analyzed and all showed altered alignment of the head around the cervical spine with hypertonus of the cricothyroid, suprahyoid, and sternocleidomastoid muscles. Although MTD is a disorder with a multifactorial etiology, the articles revealed a correlation between posture and MTD related to a forward protraction of the cervical spine with a hypertonus of the laryngeal and hyoid musculature. This study also detected that an intervention in posture could reduce vocal disorders.
Single-Image-Based 3D Reconstruction of Endoscopic Images
Authors: Ahmad Bilal , Floor Pål Anders , Farup Ivar , Andersen Casper Find
Published: March 2024

A wireless capsule endoscope (WCE) is a medical device designed for the examination of the human gastrointestinal (GI) tract. Three-dimensional models based on WCE images can assist in diagnostics by effectively detecting pathology. These 3D models provide gastroenterologists with improved visualization, particularly in areas of specific interest. However, the constraints of WCE, such as lack of controllability, and requiring expensive equipment for operation, which is often unavailable, pose significant challenges when it comes to conducting comprehensive experiments aimed at evaluating the quality of 3D reconstruction from WCE images. In this paper, we employ a single-image-based 3D reconstruction method on an artificial colon captured with an endoscope that behaves like WCE. The shape from shading (SFS) algorithm can reconstruct the 3D shape using a single image. Therefore, it has been employed to reconstruct the 3D shapes of the colon images. The camera of the endoscope has also been subjected to comprehensive geometric and radiometric calibration. Experiments are conducted on well-defined primitive objects to assess the method’s robustness and accuracy. This evaluation involves comparing the reconstructed 3D shapes of primitives with ground truth data, quantified through measurements of root-mean-square error and maximum error. Afterward, the same methodology is applied to recover the geometry of the colon. The results demonstrate that our approach is capable of reconstructing the geometry of the colon captured with a camera with an unknown imaging pipeline and significant noise in the images. The same procedure is applied on WCE images for the purpose of 3D reconstruction. Preliminary results are subsequently generated to illustrate the applicability of our method for reconstructing 3D models from WCE images.
A Study of the Relationship between the Dynamic Viscosity and Thermodynamic Properties of Palm Oil, Hydrogenated Palm Oil, Paraffin, and Their Mixtures Enhanced with Copper and Iron Fines
Authors: Dzindziora Agnieszka , Dzienniak Damian , Rokita Tomasz , Wojciechowski Jerzy , Sułowski Maciej , Nurkusheva Saltanat , Bembenek Michał
Published: March 2024

The article presents the results of phase transition studies in which the following substances and their mixtures were tested: 100% palm oil, 100% paraffin, 100% hydrogenated palm oil, 50% palm oil + 50% paraffin, 50% hydrogenated palm oil + 50% palm oil, 33% hydrogenated palm oil + 33% palm oil + 33% soft paraffin, 20% hydrogenated palm oil + 30% palm oil + 50% soft paraffin, 50% hydrogenated palm oil + 50% palm oil + copper, and 50% hydrogenated palm oil + 50% palm oil + iron. The measurements were carried out on a station for testing phase-change materials (PCMs) designed specifically for the analysis of phase changes. Viscosity values were also determined for the tested materials, and their potential impact on heat accumulation was assessed. The primary goal of the experiment was to determine some key thermodynamic parameters, including transition time, transition heat, specific heat, and dynamic viscosity at 58 °C. A one-way ANOVA test confirmed the statistical significance of minimum transition temperature, maximum transition temperature, and phase transition time, validating the reliability and utility of the results. The melting point, crucial for applications involving phase changes, was identified as an important factor. The careful selection of components allows for the customization of properties tailored to specific applications. A significant result is that the analyzed substances with higher specific heat values tend to have a higher average dynamic viscosity. The Pearson correlation coefficient of 0.82 indicated a strong positive association between the average dynamic viscosity and the heat of fusion of the substances examined. This suggests that changes in the heat of fusion significantly influence alterations in dynamic viscosity. Substances with higher specific heat values tend to exhibit higher average dynamic viscosity, emphasizing the direct impact of composition on viscosity.
Fabrication of Metal Contacts on Silicon Nanopillars: The Role of Surface Termination and Defectivity
Authors: Giulio Federico , Mazzacua Antonio , Calciati Luca , Narducci Dario
Published: March 2024

The application of nanotechnology in developing novel thermoelectric materials has yielded remarkable advancements in material efficiency. In many instances, dimensional constraints have resulted in a beneficial decoupling of thermal conductivity and power factor, leading to large increases in the achievable thermoelectric figure of merit (ZT). For instance, the ZT of silicon increases by nearly two orders of magnitude when transitioning from bulk single crystals to nanowires. Metal-assisted chemical etching offers a viable, low-cost route for preparing silicon nanopillars for use in thermoelectric devices. The aim of this paper is to review strategies for obtaining high-density forests of Si nanopillars and achieving high-quality contacts on them. We will discuss how electroplating can be used for this aim. As an alternative, nanopillars can be embedded into appropriate electrical and thermal insulators, with contacts made by metal evaporation on uncapped nanopillar tips. In both cases, it will be shown how achieving control over surface termination and defectivity is of paramount importance, demonstrating how a judicious control of defectivity enhances contact quality.
Theoretical Prediction and Experimental Synthesis of Zr3AC2 (A = Cd, Sb) Phases
Authors: Luo Jia , Zhang Fengjuan , Wen Bo , Zhang Qiqiang , Chu Longsheng , Zhou Yanchun , Feng Qingguo , Hu Chunfeng
Published: March 2024

MAX phases have great research value and application prospects, but it is challenging to synthesize the MAX phases containing Cd and Sb for the time being. In this paper, we confirmed the existence of the 312 MAX phases of Zr3CdC2 and Zr3SbC2, both from theoretical calculations and experimental synthesis. The Zr3AC2 (A = Cd, Sb) phase was predicted by the first-principles calculations, and the two MAX phases were confirmed to meet the requests of thermal, thermodynamic, and mechanical stabilities using formation energy, phonon dispersion, and the Born–Huang criteria. Their theoretical mechanical properties were also systematically investigated. It was found that the elastic moduli of Zr3CdC2 and Zr3SbC2 were 162.8 GPa and 164.3 GPa, respectively. Then, differences in the mechanical properties of Zr3AC2 (A = Cd, In, Sn, and Sb) were explained using bond layouts and charge transfers. The low theoretical Vickers hardness of the Zr3CdC2 (5.4 GPa) and Zr3SbC2 (4.3 GPa) phases exhibited excellent machinability. Subsequently, through spark plasma sintering, composites containing Zr3CdC2 and Zr3SbC2 phases were successfully synthesized at the temperatures of 850 °C and 1300 °C, respectively. The optimal molar ratio of Zr:Cd/Sb:C was determined as 3:1.5:1.5. SEM and the EDS results analysis confirmed the typical layered microstructure of Zr3CdC2 and Zr3SbC2 grains.
Environmental and Economic Viability of Using Concrete Block Wastes from a Concrete Production Plant as Recycled Coarse Aggregates
Authors: Los Santos-Ortega Jorge , Fraile-García Esteban , Ferreiro-Cabello Javier
Published: March 2024

The construction sector must incorporate the circular economy to improve sustainability and efficiency. The use of recycled aggregates (RAs) as a substitute for natural aggregates (NAs) is currently being investigated and is expected to yield considerable benefits in the future. The objective of this research is to evaluate the environmental and economic benefits of using recycled coarse aggregates (RCAs) in different 1 m3 samples of concrete, substituting the natural coarse aggregate (NCAs) with RCAs in different percentages. RCAs generally come from the treatment of construction and demolition wastes (CDWs). However, in this research, the RCAs are the concrete block wastes (CBWs) generated by a concrete production plant. Among the most notable results is that compared to concrete with no RCAs, using alternatives in which RCAs have replaced 50% of the NCAs leads to an average decrease in impact category statistics of −3.30%. In contrast to the existing literature on the subject, the process of producing RCAs generated efficiency improvements in categories such as abiotic depletion of fossil fuels (−58.72%) and global warming potential (−85.13%). This is because the transport process, a key factor in determining the viability of using RAs instead of NAs, was eliminated. In economic terms, there is a slight decrease in the financial cost of producing 1 m3 of concrete as the quantity of RCAs increases. The maximum decrease was 0.23€/m3 in the samples studied. Combining both the environmental and economic aspects resulted in a reduction factor of 0.420 g of CO2/€cent, which means fewer CO2 emissions per unit cost when using RCAs. In conclusion, these results are intended to further knowledge in the field of using RAs instead of NAs in order to help the sector achieve sustainability and find an alternative use for a particular type of business waste.
Shrimp Waste Upcycling: Unveiling the Potential of Polysaccharides, Proteins, Carotenoids, and Fatty Acids with Emphasis on Extraction Techniques and Bioactive Properties
Authors: Rossi Nicola , Grosso Clara , Delerue-Matos Cristina
Published: March 2024

Shrimp processing generates substantial waste, which is rich in valuable components such as polysaccharides, proteins, carotenoids, and fatty acids. This review provides a comprehensive overview of the valorization of shrimp waste, mainly shrimp shells, focusing on extraction methods, bioactivities, and potential applications of these bioactive compounds. Various extraction techniques, including chemical extraction, microbial fermentation, enzyme-assisted extraction, microwave-assisted extraction, ultrasound-assisted extraction, and pressurized techniques are discussed, highlighting their efficacy in isolating polysaccharides, proteins, carotenoids, and fatty acids from shrimp waste. Additionally, the bioactivities associated with these compounds, such as antioxidant, antimicrobial, anti-inflammatory, and antitumor properties, among others, are elucidated, underscoring their potential in pharmaceutical, nutraceutical, and cosmeceutical applications. Furthermore, the review explores current and potential utilization avenues for these bioactive compounds, emphasizing the importance of sustainable resource management and circular economy principles in maximizing the value of shrimp waste. Overall, this review paper aims to provide insights into the multifaceted aspects of shrimp waste valorization, offering valuable information for researchers, industries, and policymakers interested in sustainable resource utilization and waste-management strategies.
Assessment of Silver Nanoparticles Derived from Brown Algae Sargassum vulgare: Insight into Antioxidants, Anticancer, Antibacterial and Hepatoprotective Effect
Authors: Hamouda Ragaa A. , Aljohani Ebtehail S.
Published: March 2024

Algae are used as safe materials to fabricate novel nanoparticles to treat some diseases. Marine brown alga Sargassum vulgare are used to fabricate silver nanoparticles (Sv/Ag-NPs). The characterization of Sv/Ag-NPs was determined by TEM, EDX, Zeta potential, XRD, and UV spectroscopy. The Sv/Ag-NPs were investigated as antioxidant, anticancer, and antibacterial activities against Gram-positive bacteria Bacillus mojavensis PP400982, Staphylococcus caprae PP401704, Staphylococcus capitis PP402689, and Staphylococcus epidermidis PP403851. The activity of the Sv/Ag-NPs was evaluated as hepatoprotective in vitro in comparison with silymarin. The UV–visible spectrum of Sv/Ag-NPs appeared at 442 nm; the size of Sv/Ag-NPs is in range between 6.90 to 16.97 nm, and spherical in shape. Different concentrations of Sv/Ag-NPs possessed antioxidant, anticancer activities against (HepG-2), colon carcinoma (HCT-116), cervical carcinoma (HeLa), and prostate carcinoma (PC-3) with IC50 50.46, 45.84, 78.42, and 100.39 µg/mL, respectively. The Sv/Ag-NPs induced the cell viability of Hep G2 cells and hepatocytes treated with carbon tetrachloride. The Sv/Ag-NPs exhibited antibacterial activities against Staphylococcus caprae PP401704, Staphylococcus capitis PP402689, and Staphylococcus epidermidis PP403851. This study strongly suggests the silver nanoparticles derived from Sargassum vulgare showed potential hepato-protective effect against carbon tetrachloride-induced liver cells, and could be used as anticancer and antibacterial activities.
Marine Natural Products Rescuing the Eye: A Narrative Review
Authors: Lixi Filippo , Vitiello Livio , Giannaccare Giuseppe
Published: March 2024

Different degrees of visual impairment lead to a decrease in patient wellbeing, which has an adverse effect on many facets of social and professional life. Eye disorders can affect several parts of the eye, most notably the retina and the cornea, and the impacted areas might share a common form of cellular damage or dysfunction (such as inflammation, oxidative stress and neuronal degeneration). Considering that marine organisms inhabit a broad variety of marine habitats, they display a great degree of chemical diversity. As a result, molecules with a marine origin are receiving more and more attention in the hopes of developing novel therapeutic approaches. For instance, fucoxanthin has been demonstrated to be effective in protecting the retina against photo-induced damage, while largazole, astaxanthin and spirulina have all shown antioxidant, anti-inflammatory and antiapoptotic activities that can be useful for the management of several ocular diseases, such as age-related macular degeneration and ocular surface disorders. The aim of this review is to analyze the scientific literature relating to the therapeutic effects on the eye of the main natural marine products, focusing on their mechanism of action and potential clinical uses for the management of ocular diseases.
Successful Needle Aspiration of a Traumatic Pneumothorax: A Case Report and Literature Review
Authors: Bettoni Giuseppe , Gheda Silvia , Altomare Michele , Cioffi Stefano Piero Bernardo , Ferrazzi Davide , Cazzaniga Michela , Bonacchini Luca , Cimbanassi Stefania , Aseni Paolo
Published: March 2024

Traumatic pneumothorax (PTX) occurs in up to 50% of patients with severe polytrauma and chest injuries. Patients with a traumatic PTX with clinical signs of tension physiology and hemodynamic instability are typically treated with an urgent decompressive thoracostomy, tube thoracostomy, or needle decompression. There is recent evidence that non-breathless patients with a hemodynamically stable traumatic PTX can be managed conservatively through observation or a percutaneous pigtail catheter. We present here a 52-year-old woman who presented to the emergency department with a 55 mm traumatic PTX. Following aspiration of 1500 mL of air, a clinical improvement was immediately observed, allowing the patient to be discharged shortly thereafter. In hemodynamically stable patients with a post-traumatic PTX, without specific risk factors or oxygen desaturation, observation or simple needle aspiration can be a reasonable approach. Although the recent medical literature supports conservative management of small traumatic PTXs, guidelines are lacking for hemodynamically stable patients with a significantly large PTX. This case report documents our successful experience with needle aspiration in such a setting of large traumatic PTX. We aimed in this article to review the available literature on needle aspiration and conservative treatment of traumatic pneumothorax. A total of 12 studies were selected out of 190 articles on traumatic PTX where conservative treatment and chest tube decompression were compared. Our case report offers a novel contribution by illustrating the successful resolution of a sizable pneumothorax through needle aspiration, suggesting that even a large PTX in a hemodynamically stable patient, without other risk conditions, can be successfully treated conservatively with simple needle aspiration in order to avoid tube thoracostomy complications.
Catalase Detection via Membrane-Based Pressure Sensors
Authors: Bianco Monica , Zizzari Alessandra , Perrone Elisabetta , Mangiullo Diego , Mazzeo Marco , Viola Ilenia , Arima Valentina
Published: March 2024

Membrane-based sensors (MePSs) exhibit remarkable precision and sensitivity in detecting pressure changes. MePSs are commonly used to monitor catalytic reactions in solution, generating gas products crucial for signal amplification in bioassays. They also allow for catalyst quantification by indirectly measuring the pressure generated by the gaseous products. This is particularly interesting for detecting enzymes in biofluids associated with disease onset. To enhance the performance of a MePS, various structural factors influence membrane flexibility and response time, ultimately dictating the device’s pressure sensitivity. In this study, we fabricated MePSs using polydimethylsiloxane (PDMS) and investigated how structural modifications affect the Young’s modulus (E) and residual stress (σ0) of the membranes. These modifications have a direct impact on the sensors’ sensitivity to pressure variations, observed as a function of the volume of the chamber (Σ) or of the mechanical properties of the membrane itself (S). MePSs exhibiting the highest sensitivities were then employed to detect catalyst quantities inducing the dismutation of hydrogen peroxide, producing dioxygen as a gaseous product. As a result, a catalase enzyme was successfully detected using these optimized MePSs, achieving a remarkable sensitivity of (22.7 ± 1.2) µm/nM and a limit of detection (LoD) of 396 pM.
Cynaroside Induces G1 Cell Cycle Arrest by Downregulating Cell Division Cycle 25A in Colorectal Cancer
Authors: Lei Shan , Cao Wenpeng , Zeng Zhirui , Wang Lu , Lan Jinzhi , Chen Tengxiang
Published: March 2024

Natural chemicals derived from herbal plants have recently been recognized as potentially useful treatment alternatives owing to their ability to target a wide range of important biological molecules. Cynaroside is one of these natural compounds with promising anticancer activity for numerous tumor types. Nevertheless, the anticancer effects and molecular mechanisms of action of cynaroside on colorectal cancer (CRC) remain unclear. In this study, cynaroside was found to markedly inhibit CRC cell proliferation and colony formation in vitro. Cynaroside also inhibited cell proliferation in vivo and decreased the expression of KI67, a cell nuclear antigen. RNA sequencing revealed 144 differentially expressed genes (DEGs) in HCT116 cells and 493 DEGs in RKO cells that were enriched in the cell cycle signaling pathway. Cell division cycle 25A (CDC25A), a DEG widely enriched in the cell cycle signaling pathway, is considered a key target of cynaroside in CRC cells. Cynaroside also inhibited DNA replication and arrested cells in the G1/S phase in vitro. The expression levels of CDC25A and related G1-phase proteins were significantly elevated after CDC25A overexpression in CRC cells, which partially reversed the inhibitory effect of cynaroside on CRC cell proliferation and G1/S-phase arrest. In summary, cynaroside may be used to treat CRC as it inhibits CDC25A expression.
Metabolomics Characterization of Phenolic Compounds in Colored Quinoa and Their Relationship with In Vitro Antioxidant and Hypoglycemic Activities
Authors: Zhang Ling , Dang Bin , Lan Yongli , Zheng Wancai , Kuang Jiwei , Zhang Jie , Zhang Wengang
Published: March 2024

Chenopodium quinoa Willd. is rich in phenolic compounds and exhibits diverse biological activities. Few studies have focused on the effect of colored quinoa’s phenolic profile on potential biological activity. This study used a UPLC–MS/MS-based metabolomic approach to examine the quinoa phenolics and their association with in vitro antioxidant and hypoglycemic properties. In total, 430 polyphenols, mainly phenolic acids, flavonoids, and flavonols, were identified. Additionally, 121, 116, and 148 differential polyphenols were found between the white and black, white and red, and black and red comparison groups, respectively; 67 polyphenols were screened as shared key differential metabolites. Phenylalanine, tyrosine, and the biosynthesis of plant secondary metabolites were the main differently regulated pathways. Black quinoa had better total phenolic contents (643.68 mg/100 g DW) and antioxidant capacity, while white quinoa had better total flavonoid contents (90.95 mg/100 g DW) and in vitro α-amylase (IC50 value of 3.97 mg/mL) and α-glucosidase (IC50 value of 1.08 mg/mL) inhibition activities. Thirty-six polyphenols, including epicatechin and linarin, etc., were highly correlated with in vitro antioxidant activity, while six polyphenols, including tiliroside and chrysoeriol, etc., were highly correlated with in vitro hypoglycemic activity. This study may provide important information for colored quinoa resources to develop their healthy food applications.
A Paternò–Büchi Reaction of Aromatics with Quinones under Visible Light Irradiation
Authors: Li Wen-Wen , Zhao Jia-Lin , Wang Ze-Yu , Li Pei-Ting , Shi Zi-Fa , Cao Xiao-Ping , Liu Qiang
Published: March 2024

Reported herein is a Paternò–Büchi reaction of aromatic double bonds with quinones under visible light irradiation. The reactions of aromatics with quinones exposed to blue LED irradiation yielded oxetanes at −78 °C, which was attributed to both the activation of double bonds in aromatics and the stabilization of oxetanes by thiadiazole, oxadiazole, or selenadiazole groups. The addition of Cu(OTf)2 to the reaction system at room temperature resulted in the formation of diaryl ethers via the copper-catalyzed ring opening of oxetanes in situ. Notably, the substrate scope was extended to general aromatics.
Anti-Inflammatory Properties of the Citrus Flavonoid Diosmetin: An Updated Review of Experimental Models
Authors: Fang Yangyang , Xiang Wei , Cui Jinwei , Jiao Bining , Su Xuesu
Published: March 2024

Inflammation is an essential contributor to various human diseases. Diosmetin (3′,5,7-trihydroxy-4′-methoxyflavone), a citrus flavonoid, can be used as an anti-inflammatory agent. All the information in this article was collected from various research papers from online scientific databases such as PubMed and Web of Science. These studies have demonstrated that diosmetin can slow down the progression of inflammation by inhibiting the production of inflammatory mediators through modulating related pathways, predominantly the nuclear factor-κB (NF-κB) signaling pathway. In this review, we discuss the anti-inflammatory properties of diosmetin in cellular and animal models of various inflammatory diseases for the first time. We have identified some deficiencies in current research and offer suggestions for further advancement. In conclusion, accumulating evidence so far suggests a very important role for diosmetin in the treatment of various inflammatory disorders and suggests it is a candidate worthy of in-depth investigation.
Preparation of Cobalt–Nitrogen Co-Doped Carbon Nanotubes for Activated Peroxymonosulfate Degradation of Carbamazepine
Authors: Chu Bei , Tan Yixin , Lou Yichen , Lin Jiawei , Liu Yiman , Feng Jiaying , Chen Hui
Published: March 2024

Cobalt–nitrogen co-doped carbon nanotubes (Co3@NCNT-800) were synthesized via a facile and economical approach to investigate the efficient degradation of organic pollutants in aqueous environments. This material demonstrated high catalytic efficiency in the degradation of carbamazepine (CBZ) in the presence of peroxymonosulfate (PMS). The experimental data revealed that at a neutral pH of 7 and an initial CBZ concentration of 20 mg/L, the application of Co3@NCNT-800 at 0.2 g/L facilitated a degradation rate of 64.7% within 60 min. Mechanistic investigations indicated that the presence of pyridinic nitrogen and cobalt species enhanced the generation of reactive oxygen species. Radical scavenging assays and electron spin resonance spectroscopy confirmed that radical and nonradical pathways contributed to CBZ degradation, with the nonradical mechanism being predominant. This research presents the development of a novel PMS catalyst, synthesized through an efficient and stable method, which provides a cost-effective solution for the remediation of organic contaminants in water.
Estimation of Kinetics Using IMUs to Monitor and Aid in Clinical Decision-Making during ACL Rehabilitation: A Systematic Review
Authors: Krishnakumar Sanchana , van Beijnum Bert-Jan F. , Baten Chris T. M. , Veltink Peter H. , Buurke Jaap H.
Published: March 2024

After an ACL injury, rehabilitation consists of multiple phases, and progress between these phases is guided by subjective visual assessments of activities such as running, hopping, jump landing, etc. Estimation of objective kinetic measures like knee joint moments and GRF during assessment can help physiotherapists gain insights on knee loading and tailor rehabilitation protocols. Conventional methods deployed to estimate kinetics require complex, expensive systems and are limited to laboratory settings. Alternatively, multiple algorithms have been proposed in the literature to estimate kinetics from kinematics measured using only IMUs. However, the knowledge about their accuracy and generalizability for patient populations is still limited. Therefore, this article aims to identify the available algorithms for the estimation of kinetic parameters using kinematics measured only from IMUs and to evaluate their applicability in ACL rehabilitation through a comprehensive systematic review. The papers identified through the search were categorized based on the modelling techniques and kinetic parameters of interest, and subsequently compared based on the accuracies achieved and applicability for ACL patients during rehabilitation. IMUs have exhibited potential in estimating kinetic parameters with good accuracy, particularly for sagittal movements in healthy cohorts. However, several shortcomings were identified and future directions for improvement have been proposed, including extension of proposed algorithms to accommodate multiplanar movements and validation of the proposed techniques in diverse patient populations and in particular the ACL population.
Recent Progress in Wearable Near-Sensor and In-Sensor Intelligent Perception Systems
Authors: Liu Jialin , Wang Yitao , Liu Yiwei , Wu Yuanzhao , Bian Baoru , Shang Jie , Li Runwei
Published: March 2024

As the Internet of Things (IoT) becomes more widespread, wearable smart systems will begin to be used in a variety of applications in people’s daily lives, not only requiring the devices to have excellent flexibility and biocompatibility, but also taking into account redundant data and communication delays due to the use of a large number of sensors. Fortunately, the emerging paradigms of near-sensor and in-sensor computing, together with the proposal of flexible neuromorphic devices, provides a viable solution for the application of intelligent low-power wearable devices. Therefore, wearable smart systems based on new computing paradigms are of great research value. This review discusses the research status of a flexible five-sense sensing system based on near-sensor and in-sensor architectures, considering material design, structural design and circuit design. Furthermore, we summarize challenging problems that need to be solved and provide an outlook on the potential applications of intelligent wearable devices.
Piezoceramics Actuator with Attached Mass for Active Vibration Diagnostics of Reinforced Concrete Structures
Authors: Shardakov Igor , Shestakov Aleksey , Glot Irina , Gusev Georgii , Epin Valery , Tsvetkov Roman
Published: March 2024

One of the effective methods of non-destructive testing of structures is active vibration diagnostics. This approach consists of the local dynamic impact of the actuator on the structure and the registration of the vibration response. Testing of massive reinforced concrete structures is carried out with the use of actuators, which are able to create sufficiently high-impact loads. The actuators, which are based on piezoelectric elements, cannot provide a sufficient level of force and the areas where it is possible to register the vibrations excited by such actuators are quite small. In this paper, we propose a variant of a piezoactuator with attached mass, which ensures an increase in the level of dynamic impact on the structure. The effectiveness of this version is verified by numerical modeling of the dynamic interaction of the actuator with a concrete slab. The simulation was carried out within the framework of the theory of elasticity and coupled electroelasticity. An algorithm for selecting the value of the attached mass is described. It is shown that when vibrations are excited in a massive concrete slab, an actuator with an attached mass of 1.3 kg provides a 10,000-fold increase in the force compared to an actuator without attached mass. In the pulse mode, a 100-fold increase in force is achieved.
Recovery of Omega-3-Rich Lipids: Toward the Sustainable Valorization of Sea-Bass Industry Side Streams
Authors: Semenoglou Ioanna , Katsouli Maria , Giannakourou Maria , Taoukis Petros
Published: March 2024

This study pertains to the selection of the appropriate process parameters of lipid extraction aimed at the valorization of sea-bass gutting and filleting side stream biomass. A comparative kinetic study between an environmentally friendly polar (ethanol) and a conventional non-polar solvent (hexane) was implemented to determine the effect of temperature, solvent to solid ratio and time, providing insights in terms of the yield recovery and quality of omega-3 fatty acids. Maximum recovery yield (43.8 and 34.0 g/100 g of dried gutting and filleting by-products, respectively) was achieved within 10 min. In gutting by-products, ethanol achieved full lipid recovery at 35 °C and a 50:1 ratio, while for hexane, the maximum yield was 83%. In the case of filleting side-streams, hexane achieved exhaustive recovery at a 50:1 ratio and 20 °C compared with ethanol, where a higher temperature (50 °C) was needed for exhaustive recovery to be achieved. Differences were attributed to the diverse fatty acid profiles among the two by-products, with 27.3% and 40.8% polyunsaturated fatty acids in viscera and fish frames, respectively. All extracts at 20 °C and 35 °C remained below the oxidation limit set by the Codex Alimentarius Commission (peroxide value < 5 meqO2/kgoil; p-anisidine value < 20). Therefore, green solvents could assist conventional extraction, combining high efficiency and an improved environmental impact.
Wasted Potential: Decoding the Trifecta of Donor Kidney Shortage, Underutilization, and Rising Discard Rates
Authors: McKenney Ceilidh , Torabi Julia , Todd Rachel , Akhtar M. Zeeshan , Tedla Fasika M. , Shapiro Ron , Florman Sander S. , Holzner Matthew L. , van Leeuwen L. Leonie
Published: March 2024

Kidney transplantation is a life-saving intervention for end-stage renal disease; yet, the persistent gap between organ demand and supply remains a significant challenge. This paper explores the escalating discard rates of deceased donor kidneys in the United States to assess trends, discard reasons, demographical differences, and preservation techniques. Data from the Scientific Registry of Transplant Recipients from 2010 to 2021 was analyzed using chi-squared tests for trend significance and logistic regression to estimate odds ratios for kidney discard. Over the last decade, discard rates have risen to 25% in 2021. Most discarded kidneys came from extended criteria donor (ECD) donors and elevated kidney donor profile index (KDPI) scores. Kidney biopsy status was a significant factor and predictor of discard. Discard rates varied greatly between Organ Procurement and Transplantation Network regions. Of reasons for discard, “no recipient located” reached a high of 60%. Additionally, there has been a twofold increase in hypothermic machine perfusion (HMP) since 2010, with transportation difficulties being the main reason for the discard of perfused kidneys. Our findings suggest a need to recalibrate organ utilization strategies, optimize the use of lower-quality kidneys through advanced preservation methods, and address the evolving landscape of organ allocation policies to reduce kidney discard rates.
Prenatal and Postnatal Disparities in Very-Preterm Infants in a Study of Infections between 2018–2023 in Southeastern US
Authors: Dail Robin B. , Everhart Kayla C. , Iskersky Victor , Chang Weili , Fisher Kimberley , Warren Karen , Steflik Heidi J. , Hardin James W.
Published: March 2024

Background: The birthrate of Black preterm (BPT) infants is 65% higher than White preterm (WPT) infants with a BPT mortality that is 2.3 times higher. The incidence of culture-positive late-onset sepsis is as high as 41% in very-preterm infants. The main purpose of this study was to examine thermal gradients and the heart rate in relation to the onset of infection. This report presents disparities in very-preterm infection incidence, bacteria, and mortality data amongst BPT and WPT infants. Methods: 367 preterms born at <32 weeks gestational age (GA) between 2019–2023 in five neonatal intensive care units (NICUs) were enrolled to study the onset of infections and dispositions; REDCap data were analyzed for descriptive statistics. Results: The 362 infants for analyses included 227 BPTs (63.7%) and 107 WPTs (29.6%), with 28 infants of other races/ethnicities (Hispanic, Asian, and other), 50.6% female, mean GA of 27.66 weeks, and 985.24 g birthweight. BPT infants averaged 968.56 g at birth (SD 257.50), and 27.68 (SD 2.07) weeks GA, compared to WPT infants with a mean birthweight of 1006.25 g (SD 257.77, p = 0.2313) and 27.67 (SD 2.00, p = 0.982) weeks GA. Of the 426 episodes of suspected infections evaluated across all the enrolled infants, the incidence of early-onset sepsis (EOS) was 1.9%, with BPT infants having 2.50 times higher odds of EOS than WPT infants (p = 0.4130, OR (odds ratio) = 2.50, p_or = 0.408). The overall incidence of late-onset sepsis (LOS) was 10.8%, with LOS in 11.9% of BPT infants versus 9.3% (p = 0.489, OR = 1.21, p_or = 0.637) of WPT infants. BPT infants made up 69.2% of the 39 infants with Gram-positive infections vs. 25.6% for WPT infants; 16 infants had Gram-negative culture-positive infections, with 81.2% being BPT infants versus 18.8% being WPT infants. Of the 27 urinary tract infections, 78% were in BPTs. The necrotizing enterocolitis incidence was 6.9%; the incidence in BPT infants was 7.5% vs. 6.5% in WPT infants. The overall mortality was 8.3%, with BPTs at 8.4% vs. WPT infants at 9.3%, (p = 0.6715). Conclusions: BPTs had a higher rate of positive cultures, double the Gram-negative infections, a much higher rate of urinary tract infections, and a higher rate of mortality than their WPT counterparts. This study emphasizes the higher risk of morbidity and mortality for BPTs.