bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphological Comparison of the Maxillary Arch in Buccal and Palatal Canine Impaction among Asian Population of Gujarati Origin: A Hospital-Based Study
Authors: Mehta Falguni , Jain Mayank , Verma Swati , Basha Sakeenabi , Patel Renuka A. , Trivedi Rahul , Parekh Harshik A. , Ahmed Valai Kasim Shakeel , Alam Mohammad Khursheed , Nagarajappa Anil Kumar , Taneja Pratibha
Published: May 2022

Aim: To estimate the differences in the maxillary arch morphology in buccal and palatal canine impaction in an Asian population of Gujarati origin. Methodology: An institutional ethics committee’s approval was acquired before the commencement of this study. Sixty subjects were enrolled in the study. Thirty subjects (20 females and 10 males) had a maxillary impacted canine either buccal or palatal and thirty control group participants were selected aged 13 to 18 years who sought orthodontic treatment at the tertiary health care center in Ahmedabad, Gujarat, in western India. Routine pre-treatment radiographs and dental plaster models with good anatomic details were recorded. Measurements of the inter-molar width, palatal depth, arch length, sum of the mesio-distal width of the upper incisors, and available arch space were recorded from prepared orthodontic study models using digital vernier calipers with an accuracy of 0.01 mm and brass wire. The ratio of palatal depth to inter-molar width (Ratio 1), arch length to inter-molar width (Ratio 2), and width of the maxillary incisors to available arch space (Ratio 3) were also secondarily calculated. Data were analyzed using Statistical Package for Social Sciences (SPSS) version 21, IBM Inc. The normality of the data was assessed by the Shapiro–Wilk test. As the data was found to be normally distributed, bivariate analyses were also performed (one-way ANOVA test, Bonferroni post hoc correction). The level of statistical significance was set at a p-value less than 0.05. Results: The comparison of the inter-molar width, palatal depth, arch length, sum of the mesio-distal width of the upper incisors, available arch space, Ratio 1, Ratio 2, and Ratio 3 among controls and subjects with buccal and palatal canine impaction showed overall significant differences in the inter-molar width, palatal depth, arch length, sum of the mesio-distal width of the upper incisors, and available arch space when compared using one-way ANOVA as p < 0.05. Ratios 1, 2, and 3 also showed significant differences between the buccal and palatal canine impaction. Conclusion: An inadequate arch length (p < 0.0001) and a higher degree of crowding with reduced available arch space (p < 0.0001) may be considered as early risk factors for buccal maxillary canine impaction. An inadequate inter-molar width (p < 0.0001), and an increased palatal depth (p < 0.0001) with a clinically reduced mesiodistal width of the sum of maxillary incisors may be considered as risk factors for palatal maxillary canine impaction in an Asian population of Gujarati origin.
Genome-Wide Identification and Expression Analysis of AMT Gene Family in Apple (Malus domestica Borkh.)
Authors: Huang Linlin , Li Jiazhen , Zhang Bin , Hao Yanyan , Ma Fengwang
Published: May 2022

Ammonium is one of the prevalent nitrogen sources for growth and development of higher plants. Ammonium acquisition from soil is facilitated by ammonium transporters (AMTs), which are plasma membrane proteins that exclusively transport ammonium/ammonia. However, the functional characteristics and molecular mechanisms of AMTs in apple remain unclear. In this work, 15 putative AMT genes were identified and classified into four clusters (AMT1–AMT4) in apple. According to expression analysis, these AMTs had varying expressions in roots, leaves, stems, flowers and fruits. Some of them were strongly affected by diurnal cycles. AMT genes showed multiple transcript patterns to N regimes and were quite responsive to osmotic stress. In addition, phosphorylation analysis revealed that there were some conserved phosphorylation residues within the C-terminal of AMT proteins. Furthermore, detailed research was conducted on AMT1;2 functioning by heterologous expression in yeast. The present study is expected to provide basic bioinformatic information and expression profiles for the apple AMT family and to lay a basis for exploring the functional roles and regulation mechanisms of AMTs in apple.
Heterogeneity of Dyscalculia Risk Dependent on the Type of Number Line Estimation Task and the Number Magnitude
Authors: Gut Małgorzata , Mańkowska Katarzyna , Słupczewski Jakub , Matulewski Jacek
Published: May 2022

An ability that is impaired in developmental dyscalculia (DD) is related to number line estimation (NLE). However, due to variability in NLE task performance, group differences do not exemplify the real difficulty level observed in the DD population. Thirty-two of the fifty-two participants posing dyscalculia risk (DR) (mean age = 9.88) experienced difficulties in mathematics. All the children performed two number-to-position tasks and two tasks requiring a verbal estimation of a number indicated on a line, utilizing the ranges 0–100 and 0–1000. The results showed that the estimation error in the verbal task was greater in the DR group than in the typically developed (TD) group for the 0–1000 range. In the number-to-position task, group differences were found for both ranges and the variability within both groups was smaller than it was in the verbal tasks. Analyses of each of the 26 numerical magnitudes revealed a more comprehensive pattern. The majority of the group effects were related to the 0–1000 line. Therefore, considerable data variability, especially in the DD group, suggests this issue must be analyzed carefully in the case of other mathematical capacities. It also critically questions some well-established phenomena and norms in experimental and diagnostic practices.
An Exploratory Study of Shopping to Relieve Tension or Anxiety in Adolescents: Health Correlates and Gambling-Related Perceptions and Behaviors
Authors: Farhat Luis C. , Zhai Zu Wei , Hoff Rani A. , Krishnan-Sarin Suchitra , Potenza Marc N.
Published: May 2022

The desire to escape from pressures/anxiety represents an important motivation for problematic engagement with short-term rewarding behaviors that could contribute to the development of recognized and candidate behavioral addictions, including problematic shopping, a prevalent condition among youth in the U.S.A. characterized by excessive shopping cognitions/behaviors that lead to distress/impairment. However, to date, the specific correlates of shopping to relieve anxiety or tension have yet to be evaluated. We aimed at addressing this gap by analyzing data (N = 2556) from a high-school survey from Connecticut in an exploratory fashion. Adolescents who acknowledged experiencing a growing tension or anxiety that could only be relieved by shopping were classified as having negative-reinforcement shopping and compared to the remaining students. Data were analyzed in chi-square and logistic regression models to examine negative-reinforcement shopping in relation to socio-demographics, health correlates, gambling-related perceptions/attitudes, and problem-gambling severity/gambling behaviors. Negative-reinforcement shopping was more frequent in female and Hispanic students, was linked to more permissive gambling attitudes and at-risk/problematic gambling, and was associated with the use of alcohol, tobacco, marijuana, and other drugs, dysphoria/depression, and weapon-carrying and physical fighting. Taken together, these findings highlight adverse measures of health and functioning linked to negative-reinforcement shopping that may be particularly relevant to girls and Hispanic youth. Additional efforts to prevent negative outcomes are warranted.
Evaluation of Cardiovascular Pharmacotherapy Guideline Adherence and Risk Factor Control in Portuguese Community Pharmacy Patients
Authors: Fonseca Anabela , Lima Tácio de Mendonça , Fernandez-Llimos Fernando , Castel-Branco Maria Margarida , Figueiredo Isabel Vitória
Published: May 2022

Background: Cardiovascular disease (CVD) remains the leading cause of death worldwide. Assessing the patients’ CVD risk, controlling the risk factors, and ensuring the guideline-adherent cardiovascular pharmacotherapy are crucial interventions to improve health outcomes. This study aimed to evaluate the potential of pharmacists to improve the adherence to pharmacotherapy guidelines and the achievement of risk factor goals among patients who attended a community pharmacy. Methods: We conducted a single-center cross-sectional study. We performed in-pharmacy point-of-care testing, blood pressure and anthropometric measurements, and reviewed patients’ pharmacotherapy, based on European Society of Cardiology guidelines. Results: Of the 333 patients, 63.1% were in the high/very high risk category, 91.9% showed at least two modifiable risk factors, and in 61.9% of patients the cardiovascular pharmacotherapy was non-adherent to the current guidelines, failing to reach treatment goals. The lipid-lowering therapy was the least guideline adherent, with a suboptimal use of statins. However, we found no statistically significant difference between the guideline-adherent and the non-adherent group in terms of risk factor control. The pharmacist recommended 603 interventions to adhere to the guidelines. Conclusions: Community pharmacists are able to identify opportunities to optimize cardiovascular pharmacotherapy and support the patients to achieve cardiovascular risk factor goals, based on evidence-based guidelines, contributing to the improvement of CVD management.
Building the Foundation of Aquatic Literacy in 4–6 Years-Old Children: A Systematic Review of Good Pedagogical Practices for Children and Parents
Authors: Mekkaoui Léa , Schnitzler Christophe , Sidney Michel , Gandrieau Joseph , Camporelli Fabien , Potdevin François
Published: May 2022

Children between the ages of 4–6 years represent the population most affected by drowning accidents, while their early involvement in physical activity, and more specifically in aquatic activities is a key factor in their future physical life journey. The systematic review’s purpose was to identify aspects in the intervention’s studies with children and/or their parents that had a significant impact on the Aquatic Literacy (AL) dimensions mentioned as motor, psychological, affective, and cognitive. The PICO method was used to define the research question and PRISMA checklist searched for articles in nine databases: Cochrane, Embase, ERIC, ProQuest, PsychInfo, PubMed, Scopus, SportDiscus, and Web of Science. Eligibility criteria were: (1) English language, (2) primary research, (3) population of 4–6 year old children or their parents, (4) intervention study design, and (5) results related to at least one of the AL domains. The strength of evidence and the risk of bias were assessed. Results showed relatively poor number of studies for such a vulnerable population regarding the drowning risk (n = 8 for parents and n = 14 for children intervention). Studies did not show a consensus on which educational approach was more beneficial than others. Concerning parental education, results were rather homogeneous, especially concerning the theoretical frameworks employed and the relevancy to include parents in swimming programs. The development of pedagogical tools for promotion and evaluation, based on the AL theoretical framework, could help to clarify the question of “how to teach” children to prevent drowning and engage young children in long-term physical activities.
Has China’s New Round of Collective Forest Reforms Reduced Forest Fragmentation? A Case Study of the Beijing–Tianjin–Hebei Region
Authors: Zhu Shuning , Liu Jinlong , Xu Hao , Li Lingchao , Yang Wentao
Published: May 2022

The new wave of reform of collective forestland tenure (NRCFT) in China is considered an important policy for achieving sustainable management of forest resources. The purpose of this study is to investigate the influence of NRCFT on forest fragmentation in the Beijing–Tianjin–Hebei region of China based on a fixed-effects model. The forest fragmentation was analyzed based on the remote sensing images of Landsat and landscape pattern indices in the Beijing–Tianjin–Hebei region from 2000 to 2018. The results showed that (1) The NRCFT has significantly contributed to reducing forest fragmentation. (2) The effect of economic growth on forest fragmentation showed an inverted U-shape. (3) The implementation of the Grain for Green Program (GGP) and the transformation of rural energy consumption significantly reduce the degree of forest fragmentation. This study has crucial implications for formulating policies, achieving good forest governance, and reducing forest fragmentation.
Social Capital as a Predictor of Quality of Life: The Czech Experience
Authors: Murgaš František , Petrovič František , Tirpáková Anna
Published: May 2022

In the paper, we understand social capital as a variable that affects the quality of life. A variable whose change affects another variable is called a predictor. The paper is based on Putnam’s understanding of social capital with the dimensions of trust, norms and networks. Trust is considered the most important dimension, and for the purposes of the paper social capital is identified with trust. Quality of life is a holistic concept with two dimensions expressing an assessment of satisfaction with life. After society became richer—in the 1960’s in the West and, after the collapse of the bipolar world, also in Central and Eastern Europe—the need for quantity was replaced by the need for quality. The paper is focused on Czechia, with social capital as a predictor of quality of life being investigated geographically at the level of districts. According to the research hypothesis, social capital will have a strong influence on the quality of life of residents in Czechia, i.e., it will be its predictor. To test the validity of the research hypothesis, research was conducted. The aim of the paper is to outline the epistemology of social capital from the aspect of quality of life, description of quality of life and then to test the validity of the research hypothesis by measurements. The result of the quantification of social capital and quality of life at the level of districts and their correlation is important from an epistemological point of view for two reasons. The first is to question the generally accepted premise of the position of social capital as a strong predictor of quality of life. The second is the recognition that the premise of the position of social capital as a strong predictor of quality of life applies in the districts with the highest quality of life.
Dynamic Linkage between Aging, Mechanizations and Carbon Emissions from Agricultural Production
Authors: Guo Lili , Song Yuting , Zhao Shuang , Tang Mengqian , Guo Yangli , Su Mengying , Li Houjian
Published: May 2022

The trend of aging is intensifying and has become a prominent population phenomenon worldwide. The aging population has an important impact on carbon emissions, but at present, there is little research on its ecological consequences, especially the relationship with agricultural carbon emissions. For a long time, China has been dominated by a scattered small-scale peasant economy. Currently, the aging population also means that the agricultural labor force will gradually become scarce, and the agricultural production will face reform. This article is intended to find the long-term impact of aging and mechanization on agricultural carbon emissions and construct a more comprehensive policy framework for sustainable development, hoping to contribute to environmental and ecological protection. The research sample in this article is from 2000 to 2019, covering 30 provinces (cities, autonomous regions) in China. We adopted methods and models including Fully Modified General Least Squares (FMOLS), Dynamic General Least Squares (DOLS), Panel Vector Autoregression (PVAR) model, etc., and used the Granger causality test to determine the causal relationship between variables. Results show that aging is the Granger cause of agricultural carbon emissions and agricultural mechanization. Agricultural carbon emissions and agricultural mechanization have a bidirectional causal relationship. In the short term, agricultural mechanization and aging both have made a great contribution to carbon dioxide emissions from agricultural production. However, in the long term, the impact of aging on agricultural mechanization is significantly negative. Therefore, it is generally beneficial to improve the environmental problems of agricultural production. Our research focuses on the latest background of population trends and global climate issues and finally provides suggestions and a theoretical basis for the formulation of government agricultural policies according to the research conclusions.
Social Stability Risk Assessment of Disaster-Preventive Migration in Ethnic Minority Areas of Southwest China
Authors: Zhou Linyi , Zhu Demi , Shen Wei
Published: May 2022

Disaster-preventive migration (DPM) is an important method for disaster risk management, but migration itself entails a potential social stability risk. This study took County D in Yunnan Province, one of the counties most severely threatened by geological disasters in China, as an example to construct an indicator system of social stability risk factors for disaster-preventive migration based on a literature survey and in-depth interviews. The system consists of 5 first-level risk factors and 14 s-level risk factors. The social stability risk of DPM in County D was assessed using a fuzzy comprehensive evaluation method based on experts’ weights. The results showed that the overall social stability risk level of disaster-preventive migration in County D is ‘high’. In terms of importance, the five first-level risk factors were ranked as follows: public opinion risk > compensation risk > livelihood recovery risk > cultural risk > geological disaster risk. Among the risk factors, the level of public opinion risk and compensation risk appeared to be high, whereas that of livelihood recovery risk, cultural risk and geological disaster risk resulted to be medium. To our knowledge, this paper is the first research to evaluate the social stability risk of DPM; it not only enriches the theories of social stability risk assessment, but also has important guiding significance for people relocation and resettlement in Chinese ethnic minority areas.
Heavy Metals in the Mainstream Water of the Yangtze River Downstream: Distribution, Sources and Health Risk Assessment
Authors: Jin Yang , Zhou Quanping , Wang Xiaolong , Zhang Hong , Yang Guoqiang , Lei Ting , Mei Shijia , Yang Hai , Liu Lin , Yang Hui , Lv Jinsong , Jiang Yuehua
Published: May 2022

Since the mainstream of the Yangtze River lower reach is an important drinking water source for residents alongside it, it is essential to investigate the concentration, distribution characteristics and health risks of heavy metals in the water. In this study, a total of 110 water samples were collected on both the left and right banks from the upstream to the downstream. Principal component analysis (PCA) was used to determine the sources of heavy metals. Their non-carcinogenic and carcinogenic risks were studied with health risk assessment models, and uncertainties were determined through Monte Carlo simulation. Results showed that concentrations of all heavy metals were significantly lower than the relevant authoritative standards in the studied area. From the upstream to the downstream, Ni, Cu and Cr had similar concentration distribution rules and mainly originated from human industrial activities. Pb, Cd and Zn had a fluctuating but increasing trend, which was mainly due to the primary geochemistry, traffic pollution and agricultural activities. The maximum As concentration appeared in the upstream mainly because of the carbonatite weathering or mine tail water discharge. Concentrations of Zn, As, Cd and Pb on the left bank were higher than those on the right bank, while concentrations of Cu, Ni and Cr on the right bank were higher than those on the left bank. The non-carcinogenic risk index (HI) was less than 1 (except of L11), and HI on the left bank was higher than that on the right bank. The carcinogenic risk (CR) was generally larger than 1.0 × 10−4, CR on the right bank overall was higher than that on the left bank, and the health risk of kids was greater than that of adults. Furthermore, Monte Carlo simulation results and the actual calculated values were basically the same.
Molecular Dynamics—From Macromolecule to Small Molecules
Authors: Nam Ki Hyun
Published: May 2022

All natural molecules have their own physical, chemical, or biological properties and functions [...]
The DEAD-Box Protein Rok1 Coordinates Ribosomal RNA Processing in Association with Rrp5 in Drosophila
Authors: Chen Jie , Huang Yuantai , Zhang Kang
Published: May 2022

Ribosome biogenesis and processing involve the coordinated action of many components. The DEAD-box RNA helicase (Rok1) is essential for cell viability, and the depletion of Rok1 inhibits pre-rRNA processing. Previous research on Rok1 and its cofactor Rrp5 has been performed primarily in yeast. Few functional studies have been performed in complex multicellular eukaryotes. Here, we used a combination of genetics and developmental experiments to show that Rok1 and Rrp5, which localize to the nucleolus, play key roles in the pre-rRNA processing and ribosome assembly in D. melanogaster. The accumulation of pre-rRNAs caused by Rok1 depletion can result in developmental defects. The loss of Rok1 enlarged the nucleolus and led to stalled ribosome assembly and pre-rRNA processing in the nucleolus, thereby blocking rRNA maturation and exacerbating the inhibition of mitosis in the brain. We also discovered that rrp54-2/4-2 displayed significantly increased ITS1 signaling by fluorescence in situ hybridization, and a reduction in ITS2. Rrp5 signal was highly enriched in the core of the nucleolus in the rok1167/167 mutant, suggesting that Rok1 is required for the accurate cellular localization of Rrp5 in the nucleolus. We have thus uncovered functions of Rok1 that reveal important implications for ribosome processing in eukaryotes.
Bispecific Antibody-Based Immune-Cell Engagers and Their Emerging Therapeutic Targets in Cancer Immunotherapy
Authors: Shin Ha Gyeong , Yang Ha Rim , Yoon Aerin , Lee Sukmook
Published: May 2022

Cancer is the second leading cause of death worldwide after cardiovascular diseases. Harnessing the power of immune cells is a promising strategy to improve the antitumor effect of cancer immunotherapy. Recent progress in recombinant DNA technology and antibody engineering has ushered in a new era of bispecific antibody (bsAb)-based immune-cell engagers (ICEs), including T- and natural-killer-cell engagers. Since the first approval of blinatumomab by the United States Food and Drug Administration (US FDA), various bsAb-based ICEs have been developed for the effective treatment of patients with cancer. Simultaneously, several potential therapeutic targets of bsAb-based ICEs have been identified in various cancers. Therefore, this review focused on not only highlighting the action mechanism, design and structure, and status of bsAb-based ICEs in clinical development and their approval by the US FDA for human malignancy treatment, but also on summarizing the currently known and emerging therapeutic targets in cancer. This review provides insights into practical considerations for developing next-generation ICEs.
Exploring BODIPY-Based Sensor for Imaging of Intracellular Microviscosity in Human Breast Cancer Cells
Authors: Jurgutis Dziugas , Jarockyte Greta , Poderys Vilius , Dodonova-Vaitkuniene Jelena , Tumkevicius Sigitas , Vysniauskas Aurimas , Rotomskis Ricardas , Karabanovas Vitalijus
Published: May 2022

BODIPY-based molecular rotors are highly attractive imaging tools for imaging intracellular microviscosity in living cells. In our study, we investigated the ability to detect the microviscosity of biological objects by using BDP-NO2 and BDP-H molecular rotors. We describe in detail the optical properties of BDP-NO2 and BDP-H molecular rotors in aqueous media with and without proteins, together with their accumulation dynamics and localization in live and fixed human breast cancer cells. Furthermore, we investigate the applicability of these molecules to monitor microviscosity in the organelles of human breast cancer cells by fluorescence lifetime imaging microscopy (FLIM). We demonstrate that the BDP-NO2 molecular rotor aggregates in aqueous media and is incompatible with live cell imaging. The opposite effect is observed with BDP-H which preserves its stability in aqueous media, diffuses through the plasma membrane and accumulates in lipid droplets (LDs) and the cytosol of both live and fixed MCF-7 and MDA-MB-231 cancer cells. Finally, by utilizing BDP-H we demonstrate that LD microviscosity is significantly elevated in more malignant MDA-MB-231 human breast cancer cells, as compared to MCF-7 breast cancer cells. Our findings demonstrate that BDP-H is a water-compatible probe that can be successfully applied to measure microviscosity in the LDs of living cells.
In Vitro Validation of the Therapeutic Potential of Dendrimer-Based Nanoformulations against Tumor Stem Cells
Authors: Knauer Nadezhda , Arkhipova Valeria , Li Guanzhang , Hewera Michael , Pashkina Ekaterina , Nguyen Phuong-Hien , Meschaninova Maria , Kozlov Vladimir , Zhang Wei , Croner Roland , Caminade Anne-Marie , Majoral Jean-Pierre , Apartsin Evgeny , Kahlert Ulf
Published: May 2022

Tumor cells with stem cell properties are considered to play major roles in promoting the development and malignant behavior of aggressive cancers. Therapeutic strategies that efficiently eradicate such tumor stem cells are of highest clinical need. Herein, we performed the validation of the polycationic phosphorus dendrimer-based approach for small interfering RNAs delivery in in vitro stem-like cells as models. As a therapeutic target, we chose Lyn, a member of the Src family kinases as an example of a prominent enzyme class widely discussed as a potent anti-cancer intervention point. Our selection is guided by our discovery that Lyn mRNA expression level in glioma, a class of brain tumors, possesses significant negative clinical predictive value, promoting its potential as a therapeutic target for future molecular-targeted treatments. We then showed that anti-Lyn siRNA, delivered into Lyn-expressing glioma cell model reduces the cell viability, a fact that was not observed in a cell model that lacks Lyn-expression. Furthermore, we have found that the dendrimer itself influences various parameters of the cells such as the expression of surface markers PD-L1, TIM-3 and CD47, targets for immune recognition and other biological processes suggested to be regulating glioblastoma cell invasion. Our findings prove the potential of dendrimer-based platforms for therapeutic applications, which might help to eradicate the population of cancer cells with augmented chemotherapy resistance. Moreover, the results further promote our functional stem cell technology as suitable component in early stage drug development.
Olive Oil Phenols Prevent Mercury-Induced Phosphatidylserine Exposure and Morphological Changes in Human Erythrocytes Regardless of Their Different Scavenging Activity
Authors: Notariale Rosaria , Perrone Pasquale , Mele Luigi , Lettieri Gennaro , Piscopo Marina , Manna Caterina
Published: May 2022

Phosphatidylserine (PS) translocation to the external membrane leaflet represents a key mechanism in the pathophysiology of human erythrocytes (RBC) acting as an “eat me” signal for the removal of aged/stressed cells. Loss of physiological membrane asymmetry, however, can lead to adverse effects on the cardiovascular system, activating a prothrombotic activity. The data presented indicate that structurally related olive oil phenols prevent cell alterations induced in intact human RBC exposed to HgCl2 (5–40 µM) or Ca2+ ionophore (5 µM), as measured by hallmarks including PS exposure, reactive oxygen species generation, glutathione depletion and microvesicles formation. The protective effect is observed in a concentration range of 1–30 µM, hydroxytyrosol being the most effective; its in vivo metabolite homovanillic alcohol still retains the biological activity of its dietary precursor. Significant protection is also exerted by tyrosol, in spite of its weak scavenging activity, indicating that additional mechanisms are involved in the protective effect. When RBC alterations are mediated by an increase in intracellular calcium, the protective effect is observed at higher concentrations, indicating that the selected phenols mainly act on Ca2+-independent mechanisms, identified as protection of glutathione depletion. Our findings strengthen the nutritional relevance of olive oil bioactive compounds in the claimed health-promoting effects of the Mediterranean Diet.
Spontaneous Adsorption and Efficient Photodegradation of Indigo Carmine under Visible Light by Bismuth Oxyiodide Nanoparticles Fabricated Entirely at Room Temperature
Authors: Elamin Mohamed R. , Ibnaouf Khalid H. , Elamin Nuha Y. , Adam Fatima A. , Alolayan Abdulrahman H. , Abdulkhair Babiker Y.
Published: May 2022

Bismuth oxyiodide (BiOI) is a targeted material for its relative safety and photocatalytic activity under visible light. In this study, a successful simple and energy-saving route was applied to prepare BiOI through a sonochemical process at room temperature. The characterization of the prepared BiOI was conducted by physical means. The transmission electron microscope (TEM) image showed that the BiOI comprises nanoparticles of about 20 nm. Also, the surface area of the BiOI was found to be 34.03 m2 g−1 with an energy gap of 1.835 eV. The adsorption and photocatalytic capacities of the BiOI were examined for the indigo carmine dye (IC) as a model water-pollutant via the batch experiment methodology. The solution parameters were optimized, including pH, contact time, IC concentration, and temperature. Worth mentioning that an adsorption capacity of 185 mg·g−1 was obtained from 100 mg L−1 IC solution at 25 °C within 60 min as an equilibrium time. In addition, the BiOI showed a high degradation efficiency towards IC under tungsten lamb (80 W), where 93% was removed within 180 min, and the complete degradation was accomplished in 240 min. The fabricated BiOI nanoparticles completely mineralized the IC under artificial visible light, as indicated by the total organic carbon analysis.
Understanding the Role of SERCA2a Microdomain Remodeling in Heart Failure Induced by Obesity and Type 2 Diabetes
Authors: Lai Ping , Nikolaev Viacheslav O. , De Jong Kirstie A.
Published: May 2022

Obesity and type 2 diabetes (T2D) are on trend to become a huge burden across all ages. They cause harm to almost every organ, especially the heart. For decades, the incidence of heart failure with impaired diastolic function (or called heart failure with preserved ejection fraction, HFpEF) has increased sharply. More and more studies have uncovered obesity and T2D to be closely associated with HFpEF. The sarcoplasmic/endoplasmic reticulum calcium ATPase2a (SERCA2a) microdomain is a key regulator of calcium reuptake into the sarcoplasmic reticulum (SR) during diastole. 3′,5′-cyclic adenosine monophosphate (cAMP) and its downstream effector cAMP dependent protein kinase (PKA) act locally within the SERCA2a microdomain to regulate the phosphorylation state of the small regulatory protein phospholamban (PLN), which forms a complex with SERCA2a. When phosphorylated, PLN promotes calcium reuptake into the SR and diastolic cardiac relaxation by disinhibiting SERCA2a pump function. In this review, we will discuss previous studies investigating the PLN/SERCA2a microdomain in obesity and T2D in order to gain a greater understanding of the underlying mechanisms behind obesity- and T2D-induced diastolic dysfunction, with the aim to identify the current state of knowledge and future work that is needed to guide further research in the field.
The Trade-Offs/Synergies and Their Spatial-Temporal Characteristics between Ecosystem Services and Human Well-Being Linked to Land-Use Change in the Capital Region of China
Authors: Liu Mengxue , Dong Xiaobin , Wang Xuechao , Zhao Bingyu , Wei Hejie , Fan Weiguo , Zhang Chenyang
Published: May 2022

With the rise of the strategy of Coordinated Development for the Beijing-Tianjin-Hebei Region, it is necessary to evaluate the trade-offs/synergies of the survival environment and human well-being in Hebei, the capital region of China. However, existing methods cannot analyze and express trade-offs/synergies of two or more variables simultaneously. Therefore, this paper proposes a new framework to express the trade-offs/synergies among land-use intensity, ecosystem services, and human well-being. In this paper, we first identified the land-use intensity change and land-use transformation and evaluated ecosystem services and human well-being in Hebei from 2000–2015 under the Millennium Ecosystem Assessment framework. Then, the trade-offs/synergies of the three indicators were determined by GIS-based methods and MATLAB. The results show that land-use intensity and human well-being mainly present a synergistic relationship, while ecosystem services and land-use intensity mainly present a trade-off relationship, and ecosystem services and human well-being also present a trade-off relationship in Hebei during 2000–2015. In addition, some regional solutions to achieve sustainable development were proposed: region 1 needs to adjust land-use structure, region 2 needs to protect the ecological environment to improve the supply of ecosystem services, and region 3 needs to commit to improving the regional comprehensive human well-being. This study not only proposes a new framework for analyzing trade-offs/synergies of land use intensity, ecosystem services, and human well-being, but it also provides regional solutions for Hebei to achieve sustainable development.
Quantitative Depth Profiling Using Online-Laser Ablation of Solid Samples in Liquid (LASIL) to Investigate the Oxidation Behavior of Transition Metal Borides
Authors: Weiss Maximilian , Glechner Thomas , Weiss Victor U. , Riedl Helmut , Limbeck Andreas
Published: May 2022

The increased demand for sustainability requires, among others, the development of new materials with enhanced corrosion resistance. Transition metal diborides are exceptional candidates, as they exhibit fascinating mechanical and thermal properties. However, at elevated temperatures and oxidizing atmospheres, their use is limited due to the fact of their inadequate oxidation resistance. Recently, it was found that chromium diboride doped with silicon can overcome this limitation. Further improvement of this protective coating requires detailed knowledge regarding the composition of the forming oxide layer and the change in the composition of the remaining thin film. In this work, an analytical method for the quantitative measurement of depth profiles without using matrix-matched reference materials was developed. Using this approach, based on the recently introduced online-LASIL technique, it was possible to achieve a depth resolution of 240 nm. A further decrease in the ablation rate is possible but demands a more sensitive detection of silicon. Two chromium diboride samples with different Si contents suffering an oxidation treatment were used to demonstrate the capabilities of this technique. The concentration profiles resembled the pathway of the formed oxidation layers as monitored with transmission electron microscopy. The stoichiometry of the oxidation layers differed strongly between the samples, suggesting different processes were taking place. The validity of the LASIL results was cross-checked with several other analytical techniques.
(−)-Lariciresinol Isolated from the Roots of Isatis indigotica Fortune ex Lindl. Inhibits Hepatitis B Virus by Regulating Viral Transcription
Authors: Yang Lu , Wang Huiqiang , Yan Haiyan , Wang Kun , Wu Shuo , Li Yuhuan
Published: May 2022

Chronic hepatitis induced by hepatitis B virus (HBV) infection is a serious public health problem, leading to hepatic cirrhosis and liver cancer. Although the currently approved medications can reliably decrease the virus load and prevent the development of hepatic diseases, they fail to induce durable off-drug control of HBV replication in the majority of patients. The roots of Isatis indigotica Fortune ex Lindl., a traditional Chinese medicine, were frequently used for the prevention of viral disease in China. In the present study, (−)-lariciresinol ((−)-LRSL), isolated from the roots of Isatis indigotica Fortune ex Lindl., was found to inhibit HBV DNA replication of both wild-type and nucleos(t)ide analogues (NUCs)-resistant strains in vitro. Mechanism studies revealed that (−)-LRSL could block RNA production after treatment, followed by viral proteins, and then viral particles and DNA. Promoter reporter assays and RNA decaying dynamic experiments indicated that (−)-LRSL mediated HBV RNA reduction was mainly due to transcriptional inhibition rather than degradation. Moreover, (−)-LRSL in a dose-dependent manner also inhibited other animal hepadnaviruses, including woodchuck hepatitis virus (WHV) and duck hepatitis B virus (DHBV). Combining the analysis of RNA-seq, we further found that the decrease in HBV transcriptional activity by (−)-LRSL may be related to hepatocyte nuclear factor 1α (HNF1α). Taken together, (−)-LRSL represents a novel chemical entity that inhibits HBV replication by regulating HNF1α mediated HBV transcription, which may provide a new perspective for HBV therapeutics.
Anti-Inflammatory Diet for Women with Interstitial Cystitis/Bladder Pain Syndrome: The AID-IC Pilot Study
Authors: Gordon Barbara , Blanton Cynthia , Ramsey Rebekah , Jeffery Andrea , Richey Laura , Hulse Rachel
Published: May 2022

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic condition characterized by pelvic pain coupled with urinary frequency and urgency. The underlying cause of IC/BPS is unknown; there is no cure. Dietary components exacerbate symptoms. The Anti-Inflammatory Diet for Interstitial Cystitis (AID-IC) employs a randomized, crossover design to evaluate the effect of a plant-based, low saturated fat diet on the quality of life of women with IC/BPS. Insights on the implementation of the protocol and reflections on the facilitators and barriers experienced during the pilot study follow. The logistics of the protocol proved time-consuming; however, the barriers were surmountable. Quantitative and qualitative findings suggest that the AID-IC therapeutic diet may have lessened symptoms and improved the quality of life for many of the women in the study.
Effects of Baru Almond Oil (Dipteryx alata Vog.) Treatment on Thrombotic Processes, Platelet Aggregation, and Vascular Function in Aorta Arteries
Authors: Silva-Luis Cristiane Cosmo , de Brito Alves José Luiz , de Oliveira Júlio César Pinheiro Lúcio , de Sousa Luis José Alixandre , Araújo Islania Giselia Albuquerque , Tavares Josean Fechine , do Nascimento Yuri Mangueira , Bezerra Lorena Soares , Araújo de Azevedo Fátima de Lourdes Assunção , Sobral Marianna Vieira , Mangueira Vivianne Mendes , de Medeiros Isac Almeida , Veras Robson Cavalcante
Published: May 2022

Background: This study assessed the effects of Baru (Dipteryx alata Vog.) almond oil supplementation on vascular function, platelet aggregation, and thrombus formation in aorta arteries of Wistar rats. Methods: Male Wistar rats were allocated into three groups. The control group (n = 6), a Baru group receiving Baru almond oil at 7.2 mL/kg/day (BG 7.2 mL/kg, n = 6), and (iii) a Baru group receiving Baru almond oil at 14.4 mL/kg/day (BG 14.4 mL/kg, n = 6). Baru oil was administered for ten days. Platelet aggregation, thrombus formation, vascular function, and reactive oxygen species production were evaluated at the end of treatment. Results: Baru oil supplementation reduced platelet aggregation (p < 0.05) and the production of the superoxide anion radical in platelets (p < 0.05). Additionally, Baru oil supplementation exerted an antithrombotic effect (p < 0.05) and improved the vascular function of aorta arteries (p < 0.05). Conclusion: The findings showed that Baru oil reduced platelet aggregation, reactive oxygen species production, and improved vascular function, suggesting it to be a functional oil with great potential to act as a novel product for preventing and treating cardiovascular disease.
Role of Leu72Met of GHRL and Gln223Arg of LEPR Variants on Food Intake, Subjective Appetite, and Hunger-Satiety Hormones
Authors: Sanchez-Murguia Tania , Torres-Castillo Nathaly , Magaña-de la Vega Lisset , Rodríguez-Reyes Saraí Citlalic , Campos-Pérez Wendy , Martínez-López Erika
Published: May 2022

Appetite regulation has been recognized as a promising target for the prevention of obesity, which has become a worldwide health issue. Polymorphisms in the genes of hormones or receptors including Leu72Met for ghrelin and Gln223Arg for the leptin receptor could play a role in dietary intake, hunger, and satiety process. The aim of this study was to analyze subjective appetite assessments, dietary intake, and appetite hormones in relationship to these polymorphisms. Subjects (n = 132) with normal BMIs were enrolled. Dietary intake was analyzed with 3-day diet records. Subjective appetite was measured by visual analogue scales. Biochemical parameters were measured after 12 h of fasting and 120′ following ingestion of a test meal. Ghrelin and leptin levels were measured by ELISA assay (enzyme-linked immunosorbent assay) and insulin by chemiluminescence assay. The polymorphisms were determined by allelic discrimination using TaqMan® probes. Fasting ghrelin levels differed significantly between men and women. The consumption of fruit and bread/starch with added sugar servings, as indicated by dietary records, and measured ghrelin levels were higher in carriers of Leu72Met/Met72Met compared to Leu72Leu carriers; total sugar intake was higher in Gln223Gln carriers than in Gln223Arg/Arg223Arg carriers. In conclusion, the Leu72Met and Gln223Arg polymorphism in ghrelin and LEPR may contribute to differential responses to a standardized meal as evidenced by higher postprandial levels of ghrelin and may also contribute to a higher dietary sugar intake.
Three-Dimensional Printing of a Container Tablet: A New Paradigm for Multi-Drug-Containing Bioactive Self-Nanoemulsifying Drug-Delivery Systems (Bio-SNEDDSs)
Authors: Kulkarni Vineet R. , Kazi Mohsin , Shahba Ahmad Abdul-Wahhab , Radhanpuri Aakib , Maniruzzaman Mohammed
Published: May 2022

This research demonstrates the use of fused deposition modeling (FDM) 3D printing to control the delivery of multiple drugs containing bioactive self-nano emulsifying drug-delivery systems (SNEDDSs). Around two-thirds of the new chemical entities being introduced in the market are associated with some inherent issues, such as poor solubility and high lipophilicity. SNEDDSs provide for an innovative and easy way to develop a delivery platform for such drugs. Combining this platform with FDM 3D printing would further aid in developing new strategies for delivering poorly soluble drugs and personalized drug-delivery systems with added therapeutic benefits. This study evaluates the performance of a 3D-printed container system containing curcumin (CUR)- and lansoprazole (LNS)-loaded SNEDDS. The SNEDDS showed 50% antioxidant activity (IC50) at concentrations of around 330.1 µg/mL and 393.3 µg/mL in the DPPH and ABTS radical scavenging assay, respectively. These SNEDDSs were loaded with no degradation and leakage from the 3D-printed container. We were able to delay the release of the SNEDDS from the hollow prints while controlling the print wall thickness to achieve lag phases of 30 min and 60 min before the release from the 0.4 mm and 1 mm wall thicknesses, respectively. Combining these two innovative drug-delivery strategies demonstrates a novel option for tackling the problems associated with multi-drug delivery and delivery of drugs susceptible to degradation in, i.e., gastric pH for targeting disease conditions throughout the gastrointestinal tract (GIT). It is also envisaged that such delivery systems reported herein can be an ideal solution to deliver many challenging molecules, such as biologics, orally or near the target site in the future, thus opening a new paradigm for multi-drug-delivery systems.
Generation and Detection of Optical Vortices with Multiple Cascaded Spiral Phase Plates
Authors: Zhou Ziheng , Li Peng , Ma Jingbo , Zhang Shirui , Gu Yuzong
Published: May 2022

Spiral phase plate (SPP) is the widely used method in the generation of vortex beam (VB) with fixed topological charges (TCs) for specific wavelength. Although VB with large TCs can be directly generated by using the SPP with high vortex order. The fabrication of high-quality SPPs with high vortex orders usually requires complex manufacturing process and high machining accuracy. An alternative method to generate VBs with large TCs is cascaded multiple SPPs with low order. In this study, we numerically calculate the transmitted light field of cascaded multiple SPPs according to the Huygens–Fresnel diffraction integral, and perform the experimental verifications. Based on cascading 6 SPPs (3 SPPs with TCs of 2, and 3 SPPs with TCs 4, respectively), an VB with TCs as high as 18 is generated. Furthermore, The TCs of the generated VB are detected by coaxial and off-axis interfering with fundamental Gaussian beam or its conjugate beam, respectively. The generated fork and spiral patterns allow us to distinguish the value and sign of TCs carried by the VB. The experimental results coincide well with the theoretical simulations. The fork pattern shows better resolution than the spiral one, and the petal pattern with small spiral allows us to distinguish large TCs with a higher resolution.
Analysis of Optical and Near-Infrared Luminescence of Er3+ and Er3+/Yb3+ Co-Doped Heavy Metal Borate Glasses for Optical Amplifier Applications
Authors: Hegde Vinod , Devarajulu G. , Pramod A. G. , Kolavekar Sangeeta B. , Aloraini Dalal Abdullah , Almuqrin Aljawhara H. , Sayyed M. I. , Jagannath G.
Published: May 2022

For the near-infrared emission, Er3+ and Er3+/Yb3+ co-activated borate based glass hosts were synthesized by the method of melting andquenching. The emission intensity was maximum for 0.5 mol% Er3+ singly activated glass in the near-infrared (NIR) region covering the telecommunication window. The 2 mol% of Yb3+ co-doping enhanced the emission gain cross-section of the glass by two times contrast to 0.5 mol% Er3+ loaded glass. This enhancement shifted to lower spectral regions when P increased from 0 to 1. The effect of Yb3+ loading on the gain cross-section of the Er3+ co-activated glasses was analyzed using the McCumber theory. The results showed that the 0.5Er2Yb glass has a flat gain in the range of 1460–1640 nm, this suggest a lower pump threshold is enough to perform the laser functioning of a 1530 nm band and optical window of telecommunication applications.