bluuebunny/crossref_metadata_2025 huggingface dataset:
A Method for Solving the Additional Stiffness Introduced by Flexible Joints in Stewart Platform Based on FEM Modal Analysis
Authors: Zhang Tianqing , Gong Xiaoxue , Zhang Lei , Wang Yuzhe , Liu Yahui , Li Lin
Published: April 2023

The usage of flexible joints has greatly facilitated the widespread application of the Stewart construction mechanism in many advanced fields. This research focuses on the problem that the flexible joints in the Stewart mechanism cause additional stiffness effects on the whole system. A method for solving the additional stiffness of flexible joints combining the finite element method (FEM) is proposed, which avoids the complex theoretical derivation process and allows the advantages of high versatility and accuracy. Three aspects make up the main content. Firstly, based on dynamics theory, the theoretical analysis and demonstration of the method are conducted according to the symmetry characteristics of the Stewart platform. Next, the additional stiffness of a designed Stewart platform with flexible joints was solved following the method proposed, and the obtained results were verified through FEM simulation under the given conditions, which give a maximum natural frequency deviation against the theory of 2.26%. Thirdly, dynamics tests associated with the Stewart platform were conducted, and the deviation between the Stewart Platform’s natural frequencies separately obtained from the tests and the theory does not exceed 5%, which demonstrates the effectiveness and accuracy of the method in engineering applications. This study aims to provide technical support for the development of Stewart mechanisms with flexible joints.
Hydraulic Rock Drill Fault Classification Using X−Vectors
Authors: Ling Huixuan , Gao Tian , Gong Tao , Wu Jiangzhao , Zou Liang
Published: April 2023

Hydraulic rock drills are widely used in drilling, mining, construction, and engineering applications. They typically operate in harsh environments with high humidity, large temperature differences, and vibration. Under the influence of environmental noise and operational patterns, the distributions of data collected by sensors for different operators and equipment differ significantly, which leads to difficulty in fault classification for hydraulic rock drills. Therefore, an intelligent and robust fault classification method is highly desired. In this paper, we propose a fault classification technique for hydraulic rock drills based on deep learning. First, considering the strong robustness of x−vectors to the features extracted from the time series, we employ an end−to−end fault classification model based on x−vectors to realize the joint optimization of feature extraction and classification. Second, the overlapping data clipping method is applied during the training process, which further improves the robustness of our model. Finally, the focal loss is used to focus on difficult samples, which improves their classification accuracy. The proposed method obtains an accuracy of 99.92%, demonstrating its potential for hydraulic rock drill fault classification.
Deformation Prediction of Dam Based on Optimized Grey Verhulst Model
Authors: Huang Changjun , Zhou Lv , Liu Fenliang , Cao Yuanzhi , Liu Zhong , Xue Yun
Published: April 2023

Dam deformation monitoring data are generally characterized by non-smooth and no-saturated S-type fluctuation. The grey Verhulst model can get better results only when the data series is non-monotonic swing development and the saturated S-shaped sequence. Due to the limitations of the grey Verhulst model, the prediction accuracy will be limited to a certain extent. Aiming at the shortages in the prediction based on the traditional Verhulst model, the optimized grey Verhulst model is proposed to improve the prediction accuracy of the dam deformation monitoring. Compared with those of the traditional GM (1,1) model, the DGM (2,1) model, and the traditional Verhulst (1,1) model, the experimental results show that the new proposed optimized Verhulst model has higher prediction accuracy than the traditional gray model. This study offers an effective model for dealing with the non-saturated fluctuation sequence to predict dam deformation under uncertain conditions.
An Investigation into Thermal Vibrations Caused by a Moving Heat Supply on a Spinning Functionally Graded Isotropic Piezoelectric Bounded Rod
Authors: Abouelregal Ahmed E. , Marin Marin , Abusalim Sahar M.
Published: April 2023

By laminating piezoelectric and flexible materials, we can increase their performance. Therefore, the electrical and mechanical properties of layered piezoelectric materials subjected to electromechanical loads and heat sources must be analyzed theoretically and mechanically. Since the problem of infinite wave propagation cannot be addressed using classical thermoelasticity, extended thermoelasticity models have been derived. The thermo-mechanical response of a piezoelectric functionally graded (FG) rod due to a moveable axial heat source is considered in this paper, utilizing the dual-phase-lag (DPL) heat transfer model. It was supposed that the physical characteristics of the FG rod varied exponentially along the axis of the body. Both ends hold the rod, and there is no voltage across them. The Laplace transform and decoupling techniques were used to obtain the physical fields that have been analyzed. A range of heterogeneity, rotation, and heat source velocity measures were used to compare the results presented here and those in the previous literature.
The Role of C2 Domains in Two Different Phosphatases: PTEN and SHIP2
Authors: John Laura H. , Naughton Fiona B. , Sansom Mark S. P. , Larsen Andreas Haahr
Published: April 2023

Phosphatase and tensin homologue (PTEN) and SH2-containing inositol 5′-phosphatase 2 (SHIP2) are structurally and functionally similar. They both consist of a phosphatase (Ptase) domain and an adjacent C2 domain, and both proteins dephosphorylate phosphoinositol-tri(3,4,5)phosphate, PI(3,4,5)P3; PTEN at the 3-phophate and SHIP2 at the 5-phosphate. Therefore, they play pivotal roles in the PI3K/Akt pathway. Here, we investigate the role of the C2 domain in membrane interactions of PTEN and SHIP2, using molecular dynamics simulations and free energy calculations. It is generally accepted that for PTEN, the C2 domain interacts strongly with anionic lipids and therefore significantly contributes to membrane recruitment. In contrast, for the C2 domain in SHIP2, we previously found much weaker binding affinity for anionic membranes. Our simulations confirm the membrane anchor role of the C2 domain in PTEN, as well as its necessity for the Ptase domain in gaining its productive membrane-binding conformation. In contrast, we identified that the C2 domain in SHIP2 undertakes neither of these roles, which are generally proposed for C2 domains. Our data support a model in which the main role of the C2 domain in SHIP2 is to introduce allosteric interdomain changes that enhance catalytic activity of the Ptase domain.
Advances in Robotic Welding for Metallic Materials: Application of Inspection, Modeling, Monitoring and Automation Techniques
Authors: Curiel David , Veiga Fernando , Suarez Alfredo , Villanueva Pedro
Published: April 2023

The robotic welding manufacturing of metal parts is a very important process, especially in heavy industries such as shipbuilding, oil and gas, automotive, and aerospace. There is a great variety of different techniques for manufacturing by robotic welding, and the welding operations are always in a constant process of evolution, as any advance can be significant to avoid defects during the welding process. Although a great deal of research work has been carried out in recent years, thanks to which results and reviews have been presented on this subject, the main aim of this publication is to define and review works that show the advances in the main inspection, modeling, monitoring, and automated operations during the welding process to avoid, or predictively identify, any possible defect in order to obtain an optimum degree of quality in the welding.
Effect of Lean Alloyed Al and Ca on the Texture Development of Cold Rolled Mg Sheets
Authors: Delis Wassilios J. , Berners Lukas , Korte-Kerzel Sandra , Sandlöbes-Haut Stefanie
Published: April 2023

Lean alloyed Mg-Al-Ca alloys reveal weakened basal-type texture intensities and increased room-temperature ductility when compared to pure Mg. Since the combined effects of the alloying elements Al and Ca on texture evolution are not yet fully understood, in this study, two binary and seven ternary Mg-Al-Ca alloys (ranging between 0–2 wt.-% Al and 0–0.5 wt.-% Ca) were subjected to cold rolling with texture measurement after each rolling step. These measurements showed that the basal-type texture of Mg is weakened by the addition of Ca, while the addition of Al leads to stronger basal-type textures compared to the samples containing Ca. The joint effect of Al and Ca can, for specific alloy compositions, lead to a steady-state basal texture intensity, which does not become stronger with further rolling. We expect that the solubility limit of Ca in Mg affects this behaviour. For comparison, mechanical properties were obtained by compression testing, showing high degrees of deformation, of 15–25%.
Terahertz Biaxial Strain Sensor Based on Double-Upright Cross Metamaterial
Authors: Liu Yanfei , Chen Yu , Li Jing , Zhang Chunli , Wu Qiannan , Su Ningning , Li Mengwei
Published: April 2023

In this article, a terahertz metamaterial biaxial strain pressure sensor structure is proposed, which can address the problems of the low sensitivity, the narrow pressure measurement range, and the uniaxial-only detection of existing terahertz pressure sensors. The performance of the pressure sensor was studied and analyzed using the time-domain finite-element-difference method. By changing the substrate material and optimizing the structure of the top cell, the size of the structure that can simultaneously improve the range and sensitivity of the pressure measurements was determined. The simulation results show that the sensor has a pressure-sensing effect in the frequency range of 1.0–2.2 THz under the conditions of transverse electric (TE) and transverse magnetic (TM) polarization, and the sensitivity can reach up to 346 GHz/μm. The proposed metamaterial pressure sensor has significant applications in the remote monitoring of target structure deformation.
Multidrug Resistance in Critically Ill Patients: An Unresolved Issue
Authors: Spadaro Savino
Published: April 2023

Sepsis and septic shock are common in critically ill patients and, as recommended by the Surviving Sepsis Campaign (SSC), early empiric antimicrobial therapy, specifically within the first hour, is crucial for the successful management of these conditions. To be effective, the antimicrobial therapy must also be appropriately administered: the drugs should cover the most probable pathogens and achieve effective concentrations at the site of infection. However, pharmacokinetics are frequently altered in critically ill patients and continuously change since the clinical conditions of these patients quickly and markedly change over time, either improving or deteriorating. Accordingly, optimizing antimicrobial drug dosing is fundamental in intensive care units (ICUs). This Special Issue of Microorganisms examines the epidemiology, diagnostic innovations, and strategies applied in the context of infections in critically ill patients with MDR infections.
Quantitative Structure–Retention Relationship Analysis of Polycyclic Aromatic Compounds in Ultra-High Performance Chromatography
Authors: Ruggieri Fabrizio , Biancolillo Alessandra , D’Archivio Angelo Antonio , Di Donato Francesca , Foschi Martina , Maggi Maria Anna , Quattrociocchi Claudia
Published: April 2023

A comparative quantitative structure–retention relationship (QSRR) study was carried out to predict the retention time of polycyclic aromatic hydrocarbons (PAHs) using molecular descriptors. The molecular descriptors were generated by the software Dragon and employed to build QSRR models. The effect of chromatographic parameters, such as flow rate, temperature, and gradient time, was also considered. An artificial neural network (ANN) and Partial Least Squares Regression (PLS-R) were used to investigate the correlation between the retention time, taken as the response, and the predictors. Six descriptors were selected by the genetic algorithm for the development of the ANN model: the molecular weight (MW); ring descriptor types nCIR and nR10; radial distribution functions RDF090u and RDF030m; and the 3D-MoRSE descriptor Mor07u. The most significant descriptors in the PLS-R model were MW, RDF110u, Mor20u, Mor26u, and Mor30u; edge adjacency indice SM09_AEA (dm); 3D matrix-based descriptor SpPosA_RG; and the GETAWAY descriptor H7u. The built models were used to predict the retention of three analytes not included in the calibration set. Taking into account the statistical parameter RMSE for the prediction set (0.433 and 0.077 for the PLS-R and ANN models, respectively), the study confirmed that QSRR models, associated with chromatographic parameters, are better described by nonlinear methods.
Anti-SARS-CoV-2 Activity and Cytotoxicity of Amaryllidaceae Alkaloids from Hymenocallis littoralis
Authors: Le Ngoc-Thao-Hien , De Jonghe Steven , Erven Kristien , Vermeyen Tom , Baldé Aliou M. , Herrebout Wouter A. , Neyts Johan , Pannecouque Christophe , Pieters Luc , Tuenter Emmy
Published: April 2023

The Amaryllidaceae species are well-known as a rich source of bioactive compounds in nature. Although Hymenocallis littoralis has been studied for decades, its polar components were rarely explored. The current phytochemical investigation of Amaryllidaceae alkaloids from H. littoralis led to the identification of three previously undescribed compounds: O-demethyl-norlycoramine (1), (−)-2-epi-pseudolycorine (2) and (+)-2-epi-pseudolycorine (3), together with eight known compounds: 6α-hydroxyhippeastidine (4), 6β-hydroxyhippeastidine (5), lycorine (6), 2-epi-lycorine (7), zephyranthine (8), ungeremine (9), pancratistatin (10) and 9-O-demethyl-7-O-methyllycorenine (11). Among the eight previously reported compounds, five were isolated from H. littoralis for the first time (compounds 4, 5, 7, 8, and 9). Compounds 1, 4, 5, 7, 8, and 11 exhibited weak anti-SARS-CoV-2 activity (EC50 = 40–77 µM) at non-cytotoxic concentrations. Assessment of cytotoxicity on the Vero-E6 cell line revealed lycorine and pancratistatin as cytotoxic substances with CC50 values of 1.2 µM and 0.13 µM, respectively. The preliminary structure-activity relationship for the lycorine-type alkaloids in this study was further investigated, and as a result ring C appears to play a crucial role in their anti-SARS-CoV-2 activity.
Evaluation of In Vivo Prepared Albumin-Drug Conjugate Using Immunoprecipitation Linked LC-MS Assay and Its Application to Mouse Pharmacokinetic Study
Authors: Lim Jeong-Hyeon , Park Minjae , Park Yuri , Park Seo-Jin , Lee Jiyu , Hwang Sangsoo , Lee Jeongmin , Lee Yujin , Jo Eunjeong , Shin Young G.
Published: April 2023

There have been many attempts in pharmaceutical industries and academia to improve the pharmacokinetic characteristics of anti-tumor small-molecule drugs by conjugating them with large molecules, such as monoclonal antibodies, called ADCs. In this context, albumin, one of the most abundant proteins in the blood, has also been proposed as a large molecule to be conjugated with anti-cancer small-molecule drugs. The half-life of albumin is 3 weeks in humans, and its distribution to tumors is higher than in normal tissues. However, few studies have been conducted for the in vivo prepared albumin-drug conjugates, possibly due to the lack of robust bioanalytical methods, which are critical for evaluating the ADME/PK properties of in vivo prepared albumin-drug conjugates. In this study, we developed a bioanalytical method of the albumin-conjugated MAC glucuronide phenol linked SN-38 ((2S,3S,4S,5R,6S)-6-(4-(((((((S)-4,11-diethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano [3′,4′:6,7] indolizino [1,2-b] quinolin-9-yl)oxy)methyl)(2 (methylsulfonyl)ethyl)carbamoyl)oxy)methyl)-2-(2-(3-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-methylpropanamido)acetamido)phenoxy)-3,4,5-trihydroxytetra-hydro-2H-pyran-2-carboxylic acid) as a proof-of-concept. This method is based on immunoprecipitation using magnetic beads and the quantification of albumin-conjugated drug concentration using LC-qTOF/MS in mouse plasma. Finally, the developed method was applied to the in vivo intravenous (IV) mouse pharmacokinetic study of MAC glucuronide phenol-linked SN-38.
Nano-Zero-Valent Zinc-Modified Municipal Sludge Biochar for Phosphorus Removal
Authors: Zhang Yupeng , Zhang Wenbo , Zhang Hong , He Dandan
Published: April 2023

Municipal sludge biochar (MSBC) can be used to absorb phosphorus in water for waste treatment. Nano-zero-valent zinc (nZVZ) was uniformly attached to MSBC to obtain a highly efficient phosphorus-absorbing composite material, nZVZ–MSBC. Characterization by FTIR, XPS, XRD, and BET showed that nZVZ was uniformly dispersed on the surface of the MSBC. Zinc loading was able to greatly improve the adsorption performance of MSBC for phosphorus. Adsorption experiments illustrated that the adsorption process conformed to the Langmuir model, and the maximum adsorption amount was 186.5 mg/g, which is much higher than that for other municipal sludge biochars. The adsorption process reached 80% of the maximum adsorption capacity at 90 min, and this gradually stabilized after 240 min; adsorption equilibrium was reached within 24 h. The optimum pH for adsorption was 5. The main adsorption mechanism was chemical adsorption, but physical adsorption, external diffusion, internal diffusion, and surface adsorption also played roles. The potential for application as an efficient adsorbent of phosphorus from water was confirmed. In addition, a novel strategy for municipal sludge disposal and resource utilization is provided.
Radiolabeled Chalcone Derivatives as Potential Radiotracers for β-Amyloid Plaques Imaging
Authors: Capponi Pier Cesare , Mari Matteo , Ferrari Erika , Asti Mattia
Published: April 2023

Natural products often provide a pool of pharmacologically relevant precursors for the development of various drug-related molecules. In this review, the research performed on some radiolabeled chalcone derivatives characterized by the presence of the α-β unsaturated carbonyl functional group as potential radiotracers for the imaging of β-amyloids plaques will be summarized. Chalcones’ structural modifications and chemical approaches which allow their radiolabeling with the most common SPECT (Single Photon Emission Computed Tomography) and PET (Positron Emission Tomography) radionuclides will be described, as well as the state of the art regarding their in vitro binding affinity and in vivo biodistribution and pharmacokinetics in preclinical studies. Moreover, an explanation of the rationale behind their potential utilization as probes for Alzheimer’s disease in nuclear medicine applications will be provided.
Facile Attachment of Halides and Pseudohalides to Dodecaborate(2-) via Pd-catalyzed Cross-Coupling
Authors: Al-Joumhawy Mahmoud K. , Chang Jui-Chi , Sabzi Fariba , Gabel Detlef
Published: April 2023

Cross-coupling reactions with [B12H11I]2− as one partner have been used successfully for Kumada and Buchwald Hartwig couplings with Pd catalysis. Here, we found that the iodide could be substituted easily, and unexpectedly, with other halides such as Br and Cl, and with pseudohalides such as cyanide, azide, and isocyanate. We found that for Cl, Br, N3, and NCO, tetrabutylammonium salts—or sodium salts—were successful halide sources, whereas for cyanide, CuCN was the only halide source that allowed a successful exchange. The azide could be reacted further in a click reaction with triazoles. While no substitution with fluoride occurred, tetrabutylammonium fluoride in the presence of water led to [B12H11OH]2−. Yields were high to very high, and reaction times were short when using a microwave oven as a heating source.
Improvement of Network Flow Using Multi-Commodity Flow Problem
Authors: Fukugami Takato , Matsuzawa Tomofumi
Published: April 2023

In recent years, Internet traffic has increased due to its widespread use. This can be attributed to the growth of social games on smartphones and video distribution services with increasingly high image quality. In these situations, a routing mechanism is required to control congestion, but most existing routing protocols select a single optimal path. This causes the load to be concentrated on certain links, increasing the risk of congestion. In addition to the optimal path, the network has redundant paths leading to the destination node. In this study, we propose a multipath control with multi-commodity flow problem. Comparing the proposed method with OSPF, which is single-path control, and OSPF-ECMP, which is multipath control, we confirmed that the proposed method records higher packet arrival rates. This is expected to reduce congestion.
Role of Hydroxytyrosol and Oleuropein in the Prevention of Aging and Related Disorders: Focus on Neurodegeneration, Skeletal Muscle Dysfunction and Gut Microbiota
Authors: Micheli Laura , Bertini Laura , Bonato Agnese , Villanova Noemi , Caruso Carla , Caruso Maurizia , Bernini Roberta , Tirone Felice
Published: April 2023

Aging is a multi-faceted process caused by the accumulation of cellular damage over time, associated with a gradual reduction of physiological activities in cells and organs. This degeneration results in a reduced ability to adapt to homeostasis perturbations and an increased incidence of illnesses such as cognitive decline, neurodegenerative and cardiovascular diseases, cancer, diabetes, and skeletal muscle pathologies. Key features of aging include a chronic low-grade inflammation state and a decrease of the autophagic process. The Mediterranean diet has been associated with longevity and ability to counteract the onset of age-related disorders. Extra virgin olive oil, a fundamental component of this diet, contains bioactive polyphenolic compounds as hydroxytyrosol (HTyr) and oleuropein (OLE), known for their antioxidant, anti-inflammatory, and neuroprotective properties. This review is focused on brain, skeletal muscle, and gut microbiota, as these systems are known to interact at several levels. After the description of the chemistry and pharmacokinetics of HTyr and OLE, we summarize studies reporting their effects in in vivo and in vitro models of neurodegenerative diseases of the central/peripheral nervous system, adult neurogenesis and depression, senescence and lifespan, and age-related skeletal muscle disorders, as well as their impact on the composition of the gut microbiota.
Effects of Immunonutrition on Cancer Patients Undergoing Surgery: A Scoping Review
Authors: García-Malpartida Katherine , Aragón-Valera Carmen , Botella-Romero Francisco , Ocón-Bretón María Julia , López-Gómez Juan J.
Published: April 2023

Introduction: There is a large body of evidence about immunonutrition formulas; however, there are still doubts about their usefulness in routine clinical practice as compared with standard formulas. In the age of personalized medicine, new studies appear every year regarding several types of patients; therefore, an updated point of view on these formulas is necessary. Methods: The Embase database was searched from 2016 to 14 March 2022. Our criteria were articles published in English and Spanish. The evidence quality was evaluated using GRADEpro, and the review was developed according to the PRISMA statement. Results: In this review, a total of 65 unique records were retrieved; however, 36 articles did not meet the inclusion criteria and were thus excluded. In total, 29 articles were included in the final analysis. In the last few years, many meta-analyses have attempted to identify additional existing studies of surgical patients with certain pathologies, mainly oncological patients. Immunonutrition prior to oncological surgery was shown to cause a decrease in inflammatory markers in most of the studies, and the main clinical events that changed were the infectious complications after surgery. The length of stay and mortality data are controversial due to the specific risk factors associated with these events. Conclusions: The use of immunonutrition in patients who have undergone oncological surgery decreases the levels of inflammatory markers and infectious postoperative complications in almost all localizations. However, more studies are needed to assess the use of immunonutrition based on Enhanced Recovery After Surgery (ERAS) protocols.
Challenging the Myth of the Digital Native: A Narrative Review
Authors: Reid Lisa , Button Didy , Brommeyer Mark
Published: April 2023

Background and Aims: Nurses are increasingly engaging with digital technologies to enhance safe, evidence-based patient care. Digital literacy is now considered a foundational skill and an integral requirement for lifelong learning, and includes the ability to search efficiently, critique information and recognise the inherent risk of bias in information sources. However, at many universities, digital literacy is assumed. In part, this can be linked to the concept of the Digital Native, a term first coined in 2001 by the US author Marc Prensky to describe young people born after 1980 who have been surrounded by mobile phones, computers, and other digital devices their entire lives. The objective of this paper is to explore the concept of the Digital Native and how it influences undergraduate nursing education. Materials and Methods: A pragmatic approach was used for this narrative review, working forward from Prensky’s definition of the Digital Native and backward from contemporary sources of information extracted from published health, education and nursing literature. Results: The findings from this narrative review will inform further understanding of digital literacy beliefs and how these beliefs influence undergraduate nursing education. Recommendations for enhancing the digital literacy of undergraduate nursing students are also discussed. Conclusions: Digital literacy is an essential requirement for undergraduate nursing students and nurses and is linked with safe, evidence-based patient care. The myth of the Digital Native negates the reality that exposure to digital technologies does not equate digital literacy and has resulted in deficits in nursing education programs. Digital literacy skills should be a part of undergraduate nursing curricula, and National Nursing Digital Literacy competencies for entry into practice as a Registered Nurse should be developed and contextualised to individual jurisdictions.
Development and Validity of the Japanese Version of the Questionnaire on Factors That Influence Family Engagement in Acute Care Settings
Authors: Tsukuda Makoto , Ito Yoshiyasu , Kakazu Shota , Sakamoto Katsuko , Honda Junko
Published: April 2023

There exists an international consensus on the importance of family-centered care (FCC) in intensive care settings and the evaluation of collaboration between nurses and families; however, FCC is currently practiced blindly in Japan. In this study, we developed a Japanese version of the questionnaire, Factors that Influence Family Engagement (QFIFE-J) and examined its reliability and validity. A web-based survey was conducted with 250 nurses working in the intensive care unit (ICU). Exploratory and validatory factor analyses were used to ascertain factor validity. Criterion-related validity was tested using correlation analysis with the ICU Nurses’ Family Assistance Practice Scale. Internal consistency and reproducibility were verified for reliability. Following exploratory and confirmatory factor analyses, a 15-item measure emerged comprising four factors: “ICU environment”, “nurses’ attitudes”, “nurses’ workflow”, and “patient acuity”. Confirmatory factor analyses showed a generally good fit. Cronbach’s α for the overall scale was 0.78, indicating acceptable internal consistency. The intraclass coefficient for test–retest reliability was 0.80. It was found that the QFIFE-J was reliable and valid and may help determine the factors that promote or inhibit FCC. Additionally, this study has also clarified the current status and family support related issues in ICUs in Japan.
Phenotypic and Genotypic Characterization of Methicillin Resistance in Staphylococci Isolated from an Egyptian University Hospital
Authors: Abdelwahab Marwa A. , Amer Wesam H. , Elsharawy Dalia , Elkolaly Reham M. , Helal Rehab Abd El Fattah , El Malla Dina Ahmed , Elfeky Yomna G. , Bedair Hebatallah A. , Amer Rania S. , Abd-Elmonsef Marwa E. , Taha Marwa S.
Published: April 2023

Methicillin-resistant in Staphylococci is a serious public health issue. It is mostly encoded by the mecA gene. The mecC gene is a new mecA analog responsible for resistance to methicillin in some Staphylococcal clinical isolates. This mecC gene is still underestimated in Egypt. The aim of the current study was to detect mecA and mecC genes in clinical Staphylococci isolates from a tertiary care university hospital in Egypt compared to the different phenotypic methods. A total of 118 Staphylococcus aureus (S. aureus) and 43 coagulase-negative Staphylococci (CoNS) were identified from various hospital-acquired infections. Methicillin resistance was identified genotypically using the PCR technique and phenotypically using the cefoxitin disc diffusion test, oxacillin broth microdilution and the VITEK2 system in all Staphylococcal isolates. The mecA gene was detected in 82.2% of S. aureus and 95.3% of CoNS isolates, while all of the isolates tested negative for the mecC gene. Interestingly, 30.2% of CoNS isolates showed the unique character of inducible oxacillin resistance, being mecA-positive but oxacillin-susceptible (OS-CoNS). The dual use of genotypic and phenotypic methods is highly recommended to avoid missing any genetically divergent strains.
Ciprofloxacin-Loaded Mixed Polymeric Micelles as Antibiofilm Agents
Authors: Stancheva Rumena , Paunova-Krasteva Tsvetelina , Topouzova-Hristova Tanya , Stoitsova Stoyanka , Petrov Petar , Haladjova Emi
Published: April 2023

In this work, mixed polymeric micelles (MPMs) based on a cationic poly(2-(dimethylamino)ethyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA29-b-PCL70-b-PDMAEMA29) and a non-ionic poly(ethylene oxide)–b-poly(propylene oxide)–b-poly(ethylene oxide) (PEO99-b-PPO67-b-PEO99) triblock copolymers, blended at different molar ratios, were developed. The key physicochemical parameters of MPMs, including size, size distribution, and critical micellar concentration (CMC), were evaluated. The resulting MPMs are nanoscopic with a hydrodynamic diameter of around 35 nm, and the ζ-potential and CMC values strongly depend on the MPM’s composition. Ciprofloxacin (CF) was solubilized by the micelles via hydrophobic interaction with the micellar core and electrostatic interaction between the polycationic blocks, and the drug localized it, to some extent, in the micellar corona. The effect of a polymer-to-drug mass ratio on the drug-loading content (DLC) and encapsulation efficiency (EE) of MPMs was assessed. MPMs prepared at a polymer-to-drug mass ratio of 10:1 exhibited very high EE and a prolonged release profile. All micellar systems demonstrated their capability to detach pre-formed Gram-positive and Gram-negative bacterial biofilms and significantly reduced their biomass. The metabolic activity of the biofilm was strongly suppressed by the CF-loaded MPMs indicating the successful drug delivery and release. The cytotoxicity of empty and CF-loaded MPMs was evaluated. The test reveals composition-dependent cell viability without cell destruction or morphological signs of cell death.
A Small Molecule Targeting the Intracellular Tyrosine Kinase Domain of ROR1 (KAN0441571C) Induced Significant Apoptosis of Non-Small Cell Lung Cancer (NSCLC) Cells
Authors: Ghaderi Amineh , Okhovat Mohammad-Ali , Lehto Jemina , De Petris Luigi , Manouchehri Doulabi Ehsan , Kokhaei Parviz , Zhong Wen , Rassidakis Georgios Z. , Drakos Elias , Moshfegh Ali , Schultz Johan , Olin Thomas , Österborg Anders , Mellstedt Håkan , Hojjat-Farsangi Mohammad
Published: April 2023

The ROR1 receptor tyrosine kinase is expressed in embryonic tissues but is absent in normal adult tissues. ROR1 is of importance in oncogenesis and is overexpressed in several cancers, such as NSCLC. In this study, we evaluated ROR1 expression in NSCLC patients (N = 287) and the cytotoxic effects of a small molecule ROR1 inhibitor (KAN0441571C) in NSCLC cell lines. ROR1 expression in tumor cells was more frequent in non-squamous (87%) than in squamous (57%) carcinomas patients, while 21% of neuroendocrine tumors expressed ROR1 (p = 0.0001). A significantly higher proportion of p53 negative patients in the ROR1+ group than in the p53 positive non-squamous NSCLC patients (p = 0.03) was noted. KAN0441571C dephosphorylated ROR1 and induced apoptosis (Annexin V/PI) in a time- and dose-dependent manner in five ROR1+ NSCLC cell lines and was superior compared to erlotinib (EGFR inhibitor). Apoptosis was confirmed by the downregulation of MCL-1 and BCL-2, as well as PARP and caspase 3 cleavage. The non-canonical Wnt pathway was involved. The combination of KAN0441571C and erlotinib showed a synergistic apoptotic effect. KAN0441571C also inhibited proliferative (cell cycle analyses, colony formation assay) and migratory (scratch wound healing assay) functions. Targeting NSCLC cells by a combination of ROR1 and EGFR inhibitors may represent a novel promising approach for the treatment of NSCLC patients.
Cannabinoids and Multiple Sclerosis: A Critical Analysis of Therapeutic Potentials and Safety Concerns
Authors: Nouh Roua A. , Kamal Ahmed , Abdelnaser Anwar
Published: April 2023

Multiple sclerosis (MS) is a complicated condition in which the immune system attacks myelinated axons in the central nervous system (CNS), destroying both myelin and axons to varying degrees. Several environmental, genetic, and epigenetic factors influence the risk of developing the disease and how well it responds to treatment. Cannabinoids have recently sparked renewed interest in their therapeutic applications, with growing evidence for their role in symptom control in MS. Cannabinoids exert their roles through the endogenous cannabinoid (ECB) system, with some reports shedding light on the molecular biology of this system and lending credence to some anecdotal medical claims. The double nature of cannabinoids, which cause both positive and negative effects, comes from their actions on the same receptor. Several mechanisms have been adopted to evade this effect. However, there are still numerous limitations to using cannabinoids to treat MS patients. In this review, we will explore and discuss the molecular effect of cannabinoids on the ECB system, the various factors that affect the response to cannabinoids in the body, including the role of gene polymorphism and its relation to dosage, assessing the positive over the adverse effects of cannabinoids in MS, and finally, exploring the possible functional mechanism of cannabinoids in MS and the current and future progress of cannabinoid therapeutics.
Broadband UV Supercontinuum Generation by Three-Color Ionizing Laser Pulses
Authors: Laryushin Ivan , Romanov Alexander
Published: April 2023

We theoretically investigated the properties of the ultraviolet secondary radiation generated under the action of three-color ionizing pulses obtained from parametric light generators. We show that the combination components in the spectrum of electron currents generated under the action of such three-color pulses can form a strictly exponentially decreasing sequence. For moderately short pump pulse durations (30 fs at 800 nm main field) with the use of this effect, it is possible to generate a smooth broadband supercontinuum in the 800–100 nm range.
Interpretation of Localized Surface Plasmonic Resonances of Gold Nanoparticles Covered by Polymeric Coatings
Authors: Stamatelatos Alkeos , Tsarmpopoulou Maria , Geralis Dimitrios , Chronis Alexandros G. , Karoutsos Vagelis , Ntemogiannis Dimitrios , Maratos Dionysios M. , Grammatikopoulos Spyridon , Sigalas Mihail , Poulopoulos Panagiotis
Published: April 2023

Plasmonic materials currently have a plethora of applications. How would a dielectric matrix, such as diblock copolymers, tune plasmonic properties? In this work, self-assembled gold nanoparticles were fabricated in medium vacuum conditions on heated Corning glass substrates (kept at 440 °C) under the coexistence of argon and air by means of DC magnetron sputtering. These samples were compared structurally and optically to samples deposited at room temperature and post annealed. Subsequently, the better of the two preparations, those deposited on heated glass, were covered with three different polymers, namely: Polystyrene-block-polybutadiene-block-polystyrene (PS-b-PBD-b-PS); Polystyrene-co-methyl methacrylate (PS-co-PMMA); and Polystyrene-block-polyisoprene-block-polystyrene (PS-b-PI-b-PS), by means of spin coating. Localized surface plasmon resonances were recorded and analyzed, respectively, for polymer-covered gold nanoparticles, with the width, intensity, and position of the resonances changing according to multiple factors, such as the nanoparticles size and the refractive index of each polymer. Lastly, for purposes of justification and comparison with the experimental results, rigorous theoretical calculations have been carried out.
Spatial Distribution Characteristics of Suitable Planting Areas for Pyrus Species under Climate Change in China
Authors: Wang Mi , Hu Zhuowei , Wang Yongcai , Zhao Wenji
Published: April 2023

Planting suitability determines the distribution and yield of crops in a given region which can be greatly affected by climate change. In recent years, many studies have shown that carbon dioxide fertilization effects increase the productivity of temperate deciduous fruit trees under a changing climate, but the potential risks to fruit tree planting caused by a reduction in suitable planting areas are rarely reported. In this study, Maxent was first used to investigate the spatial distribution of five Pyrus species in China, and the consistency between the actual production area and the modeled climatically suitable area under the current climatic conditions were determined. In addition, based on Coupled Model Intercomparison Project Phase 6, three climate models were used to simulate the change in suitable area and the migration trend for different species under different emission scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0 and SSP5-8.5). The results showed that the suitable area for pear was highly consistent with the actual main production area under current climate conditions. The potential planting areas of P. ussuriensis showed a downward trend under all emission paths from 2020 to 2100; other species showed a trend of increasing first and then decreasing or slowing down and this growth effect was the most obvious in 2020–2040. Except for P. pashia, other species showed a migration trend toward a high latitude, and the trend was more prominent under the high emission path. Our results emphasize the response difference between species to climate change, and the method of consistency analysis between suitable planting area and actual production regions cannot only evaluate the potential planting risk but also provide a reasonable idea for the accuracy test of the modeled results. This work has certain guiding and reference significance for the protection of pear germplasm resources and the prediction of yield.
Pressure Drop Dynamics during Filtration of Mixture Aerosol Containing Water, Oil, and Soot Particles on Nonwoven Filters
Authors: Kamiński Mateusz , Gac Jakub M. , Sobiech Piotr , Kozikowski Paweł , Jankowski Tomasz
Published: April 2023

The pressure drop dynamics during the filtration of three-component mixture aerosols are investigated and compared with two and single-component aerosols. The main area of interest is the effect of the addition of a small quantity of liquid (oil) and solid (soot) particles during the filtration of aerosol containing water mist. In addition, calculations of the change in filter mass during oil aerosol filtration have been carried out and compared with the experimental results. The new, improved filtration efficiency model takes into account a better coefficient fitting in the filtration mechanism equations. The limitations in the change in fibre diameter and packing density resulting from the filter loading have been implemented in the model. Additionally, the calculation model employs the fibre size distribution representation via multiple average fibre diameters. The changes in fibre diameter are dependent on each fibre’s calculated filtration efficiency. The improved filtration model has been utilised to predict the mass change of the filters during the filtration of pure and mixture aerosols. The pressure drop calculation model based on changes in filter mass has been formulated. The model is then utilised to calculate pressure drop changes resulting from the filtration of the oil aerosol and water and oil mixture aerosol.
Aging of Wood for Musical Instruments: Analysis of Changes in Color, Surface Morphology, Chemical, and Physical-Acoustical Properties during UV and Thermal Exposure
Authors: Gurau Lidia , Timar Maria Cristina , Cosereanu Camelia , Cosnita Mihaela , Stanciu Mariana Domnica
Published: April 2023

The acoustic features of old resonance wood in violins exhibit a superior quality when compared to those from new resonance wood. This study focuses on an assessment of the sound quality of two types of wood for musical instruments, spruce and maple (class A and D), before and after aging via thermal and UV exposure. The samples were characterized before and after UV aging in terms of color change (using a Chroma meter), surface morphology (using a MarSurf XT20 instrument), chemical changes (monitored by FTIR spectroscopy), and sound propagation speed (using an ultrasound device). After UV treatment, the wavier surface increased the area of exposure and degradation. Also, the color changes were found to be more accentuated in the case of spruce compared to sycamore maple. The FTIR results indicated more advanced aging processes for spruce when compared to maple under the same experimental conditions. This difference resulted mostly from the increased formation of carbonyl-containing chromophores via oxidative processes in spruce rather than in maple, which is in agreement with the color change findings. Exposure of both species to thermal and UV radiation led to an increase in sound propagation speed, both longitudinally and radially, and to a greater extent in wood quality class A when compared to quality class D.
Design and Optimization of the Training Device for the Employment of Hydraulic Rescue Tools in Traffic Accidents
Authors: Ballay Michal , Leitner Bohuš , Jakubovičová Lenka
Published: April 2023

This paper is concerned with the design and structural optimization of a training device for operators of a hydraulic rescue tool employed during traffic accidents, in conjunction with the improvement of the technical procedures used in such situations. Changes in the design process and subsequent production in the motor industry frequently result in an increased impact resistance of the used structural components. This applies, also, to extrication works and frequently used technical equipment. This paper presents its findings on the design process for the prototype of a training device designed for the extrication cutting drill with the assistance of a hydraulic rescue tool. The primary part of the research was dedicated to structural optimization; therefore, parameter dimensioning of the training device’s prototype was implemented. The device’s mechanical resistance, sturdiness, and stability during the implementation of hydraulic tools were also taken into account. A secondary part of this research comprised experimental results aimed at assessing the time needed to cut through the structural parts of a vehicle—pillars “A” and “B”—while using a hydraulic rescue tool. The structural design of the pillars of selected mid-range vehicles, according to their year of manufacture, was employed. The experiment showed that the newer the vehicle, the higher the cutting resistance of the pillars (predominantly “B”-type pillars). The results revealed that the cutting-work drill contributes to the reduction in the actual cutting time. Furthermore, the identification of the optimal place for cutting and the cutting angle led to more efficient extrication processes that can be applied during rescue works resulting from traffic accidents.
Analysis of Regulating Valve Stem Fracture in a Petrochemical Plant
Authors: Guo Fuping , Lyu Yunrong , Duan Zhihong , Fan Zhiqing , Li Weiming , Chen Falin
Published: April 2023

This paper investigates the failure of a regulating valve stem in a petrochemical plant, which was mainly caused by vibration fatigue under small opening conditions. The fractured valve stem was analyzed using macroscopic analysis, chemical composition analysis, mechanical property analysis, metallographic analysis, fracture surface observation, and energy spectrum analysis. Additionally, fluid-structure interaction (FSI) modal analysis was used to investigate the failure of the regulating valve. The results indicate that the valve opening had a direct impact on the vibration of the valve body, which, when operated at small openings, led to fatigue fracture at the step of variable cross-section. The paper suggests a smooth transition treatment be performed at the variable cross-section of the valve stem to avoid stress concentration. Although this study is limited to a specific case, it provides valuable insights for the failure analysis of valves operating at small openings.
Complicated Time-Constrained Project Scheduling Problems in Water Conservancy Construction
Authors: Zhang Song , Song Xiaokang , Shen Liang , Xu Lichun
Published: April 2023

Water conservancy project scheduling is an extension to the classic resource-constrained project scheduling problem (RCPSP). It is limited by special time constraints called “forbidden time windows” during which certain activities cannot be executed. To address this issue, a specific RCPSP model is proposed, and an approach is designated for it which incorporates both a priority rule-based heuristic algorithm to obtain an acceptable solution, and a hybrid genetic algorithm to further improve the quality of the solution. In the genetic algorithm, we introduce a new crossover operator for the forbidden time window and adopt double justification and elitism strategies. Finally, we conduct simulated experiments on a project scheduling problem library to compare the proposed algorithm with other priority-rule based heuristics, and the results demonstrate the superiority of our algorithm.