bluuebunny/crossref_metadata_2025 huggingface dataset:
Key Technologies and Economic Analysis of Decentralized Wind Power Consumption: A Case Study in B City, China
Authors: Huang Hui , Du Yingying , Song Shizhong , Guo Yanlei
Published: August 2020

Wind power technology is developing rapidly in China. Especially in the north of China, decentralized wind power (DWP) has developed rapidly. However, wind curtailment exceeds 4% of the gross generation, which has become an important obstacle restricting the development of DWP in 2019. Technical schemes are effective solutions to promote decentralized wind power consumption (DWPC). Moreover, there are few economic analyses of DWP projects with key technologies. This paper summarizes the typical technical schemes and analyzes the economy of a DWP grid-connected project with typical technical schemes by combining methods of the net present value (NPV) and real options (RO). It appears that the DWP project can be profitable with an NPV of about CNY 4.3721 million and an option value of CNY 16.6022 million in the case of a feed-in tariff of 0.34 CNY/kWh. Feed-in tariff and equipment investment costs are the most relevant parameters for economic feasibility according to the sensitivity analysis. Furthermore, 30.73 million kWh connects to the Mengxi grid each year. The project has an annual maximum CO2 emission reduction of about 31 thousand tons and an SO2 of about 0.1 thousand tons. In addition, it also realizes fine social benefits, including the support of local residents and the improvement of local new urbanization levels. Finally, this paper puts forward policy suggestions for the development of DWP mainly from an economic point of view.
Insights into the Aeroacoustic Noise Generation for Vertical Axis Turbines in Close Proximity
Authors: Viqueira-Moreira Manuel , Ferrer Esteban
Published: August 2020

We present Large Eddy Simulations and aeroacoustic spectra for three configurations of increasing flow complexity: an isolated NACA0012 airfoil, an isolated rotating vertical axis wind turbine composed of three rotating airfoils and a farm of four vertical axis turbines (with identical characteristics as the isolated turbine), which are located in close proximity. The aeroacoustic signatures of the simulated airfoil and the isolated turbine are validated using published numerical and experimental data. We provide theoretical estimates to predict tonal frequencies, which are used to identify the main physical mechanisms responsible for the tonal signature and for each configuration and enable the categorisation of the main tonal aeroacoustic sources of vertical axis turbines operating in close proximity. Namely, we identify wake, vortex, blade passing and boundary layer phenomena and provide estimates for the associated tonal frequencies, which are validated with simulations. In the farm, we observe non-linear interactions and enhanced mixing that decreases tonal frequencies in favour of larger broadband amplitudes at low frequencies. Comparing the spectrum with that of the isolated turbine, only the blade passing frequency and the boundary layer tones can be clearly identified. Variations in acoustic amplitudes, tonal frequencies and sound directivities suggest that a linear combination of sources from isolated turbines is not enough to characterise the aeroacoustic footprint of vertical axiswind turbines located in close proximity, and that farms need to be considered and studied as different entities.
Economic Issues in Deep Low-Carbon Energy Systems
Authors: Mauleón Ignacio
Published: August 2020

The main interlinked challenges to achieve a low-carbon emission economy are analyzed. It is argued first that there are no obstacles to a free market working effectively with a high penetration of distributed Renewable Energies (RE), since intermittency has been overstated, and affordable storage solutions are available because of strong learning rates. Demand-side management policies are promising too, neither are there foreseeable boundaries to the availability of economically extractable photovoltaic and wind energies. A full 100% RE system may be more challenging though, partly because bioenergy, a key dispatchable source in most available RE roadmaps, clashes with growing food needs and reforestation to counter greenhouse gases emissions. Similarly, the green growth proposal is constrained by materials availability, mainly cobalt and phosphorus, which will also constrain the deployment of electric vehicles. Alternatively, the United Nations Human Development Index may be a more suitable target for a sustainable RE system. Although history is not reassuring, the main global economic hurdle is possibly existing fossil fuel-related investments, likely to become stranded. An assessment of their value yields a substantially lower figure than is sometimes claimed, though. Finally, a limited role for nuclear energy is assessed positively, provided it is publicly owned.
Seasonal Variation of Chemical Composition, Fatty Acid Profile, and Sensory Properties of a Mountain Pecorino Cheese
Authors: Serrapica Francesco , Masucci Felicia , Di Francia Antonio , Napolitano Fabio , Braghieri Ada , Esposito Giulia , Romano Raffaele
Published: August 2020

This study aims to assess the compositional traits and sensory characteristics of a traditional pecorino cheese associated with management and feeding system seasonality. The study was carried out on two mountain dairy farms using an outdoor, pasture-based system from April to October (OutS), and an indoor system (InS) during the rest of the year. Outdoor-produced milk had higher fat content and a tendency for protein and somatic cell count to be higher. The OutS cheeses showed higher dry matter and fat content, higher percentages of unsaturated fatty acids, C18:3, cis-9, trans-11 conjugated linoleic acid, and trans-11 C18:1, and lower percentages of C14:0 and C16:0. These modifications in fatty acid composition determined the reduction of the atherogenic index. The OutS cheeses also displayed higher intensity of almost all sensory attributes, including odor, flavor, taste, and texture descriptors. The outdoor system partly reduced the liking of consumers for pecorino. However, changes in the productive process leading to an increment in the water content and softness of the cheeses (i.e., controlled humidity and temperature during ripening) may increase the overall liking of pasture-based products, thus promoting the consumption of healthier foods.
CRISPR/Cas9 in Cancer Immunotherapy: Animal Models and Human Clinical Trials
Authors: Khalaf Khalil , Janowicz Krzysztof , Dyszkiewicz-Konwińska Marta , Hutchings Greg , Dompe Claudia , Moncrieff Lisa , Jankowski Maurycy , Machnik Marta , Oleksiewicz Urszula , Kocherova Ievgeniia , Petitte Jim , Mozdziak Paul , Shibli Jamil A. , Iżycki Dariusz , Józkowiak Małgorzata , Piotrowska-Kempisty Hanna , Skowroński Mariusz T. , Antosik Paweł , Kempisty Bartosz
Published: August 2020

Even though chemotherapy and immunotherapy emerged to limit continual and unregulated proliferation of cancer cells, currently available therapeutic agents are associated with high toxicity levels and low success rates. Additionally, ongoing multi-targeted therapies are limited only for few carcinogenesis pathways, due to continually emerging and evolving mutations of proto-oncogenes and tumor-suppressive genes. CRISPR/Cas9, as a specific gene-editing tool, is used to correct causative mutations with minimal toxicity, but is also employed as an adjuvant to immunotherapy to achieve a more robust immunological response. Some of the most critical limitations of the CRISPR/Cas9 technology include off-target mutations, resulting in nonspecific restrictions of DNA upstream of the Protospacer Adjacent Motifs (PAM), ethical agreements, and the lack of a scientific consensus aiming at risk evaluation. Currently, CRISPR/Cas9 is tested on animal models to enhance genome editing specificity and induce a stronger anti-tumor response. Moreover, ongoing clinical trials use the CRISPR/Cas9 system in immune cells to modify genomes in a target-specific manner. Recently, error-free in vitro systems have been engineered to overcome limitations of this gene-editing system. The aim of the article is to present the knowledge concerning the use of CRISPR Cas9 technique in targeting treatment-resistant cancers. Additionally, the use of CRISPR/Cas9 is aided as an emerging supplementation of immunotherapy, currently used in experimental oncology. Demonstrating further, applications and advances of the CRISPR/Cas9 technique are presented in animal models and human clinical trials. Concluding, an overview of the limitations of the gene-editing tool is proffered.
Impacts of COVID-19 on Trade and Economic Aspects of Food Security: Evidence from 45 Developing Countries
Authors: Erokhin Vasilii , Gao Tianming
Published: August 2020

The stability of food supply chains is crucial to the food security of people around the world. Since the beginning of 2020, this stability has been undergoing one of the most vigorous pressure tests ever due to the COVID-19 outbreak. From a mere health issue, the pandemic has turned into an economic threat to food security globally in the forms of lockdowns, economic decline, food trade restrictions, and rising food inflation. It is safe to assume that the novel health crisis has badly struck the least developed and developing economies, where people are particularly vulnerable to hunger and malnutrition. However, due to the recency of the COVID-19 problem, the impacts of macroeconomic fluctuations on food insecurity have remained scantily explored. In this study, the authors attempted to bridge this gap by revealing interactions between the food security status of people and the dynamics of COVID-19 cases, food trade, food inflation, and currency volatilities. The study was performed in the cases of 45 developing economies distributed to three groups by the level of income. The consecutive application of the autoregressive distributed lag method, Yamamoto’s causality test, and variance decomposition analysis allowed the authors to find the food insecurity effects of COVID-19 to be more perceptible in upper-middle-income economies than in the least developed countries. In the latter, food security risks attributed to the emergence of the health crisis were mainly related to economic access to adequate food supply (food inflation), whereas in higher-income developing economies, availability-sided food security risks (food trade restrictions and currency depreciation) were more prevalent. The approach presented in this paper contributes to the establishment of a methodology framework that may equip decision-makers with up-to-date estimations of health crisis effects on economic parameters of food availability and access to staples in food-insecure communities.
Correlation of PTC Taste Status with Fungiform Papillae Count and Body Mass Index in Smokers and Non-Smokers of Eastern Province, Saudi Arabia
Authors: Khan Asim Mustafa , Al-Jandan Badr , Bugshan Amr , Al-Juaid Khalid , Ali Saqib , Jameela Reshma Veerankutty , Al Madan Nasser , BuHulaiga Alaa
Published: August 2020

Phenylthiocarbamide (PTC) is a bitter compound that is similar in taste to the polyphenols present in most vegetables and fruits. The human taste response towards this compound influences dietary preference, which has a bearing on an individual’s body mass index (BMI). Another factor that influences taste perception is fungiform papillae count. This, in turn, is governed by genetic factors or deleterious habits such as smoking. Establishing a link between all the above factors could lead to a wider understanding of obesity, which is a global health issue. PTC taste response, BMI, and fungiform papillae were recorded and statistically analyzed between two groups—smokers and nonsmokers. There was no statistically significant difference between smokers and nonsmokers with regard to PTC tasting ability. However, there was a significant inverse relationship between BMI and PTC tasting ability and fungiform papillae count both in smokers and nonsmokers. Thus, it can be inferred that as BMI increases, there is a lower likelihood of experiencing the bitter taste of PTC. Additionally, the ability to taste PTC decreases with diminishing numbers of fungiform papillae. Smoking does not affect bitter PTC tasting ability despite negatively affecting fungiform papillae count.
Assessment in the Supine-To-Stand Task and Functional Health from Youth to Old Age: A Systematic Review
Authors: Cattuzzo Maria Teresa , de Santana Frederico Santos , Safons Marisete Peralta , Ré Alessandro Hervaldo Nicolai , Nesbitt Danielle Rene , Santos Ariane Brito Diniz , Feitoza Anderson Henry Pereira , Stodden David Franklin
Published: August 2020

Performance in the supine-to-stand (STS) task is an important functional and health marker throughout life, but the evaluation methods and some correlates can impact it. This article aims to examine the studies that assessed the performance of the STS task of young people, adults and the elderly. Evidence of the association between the STS task and body weight status, musculoskeletal fitness and physical activity was investigated, and a general protocol was proposed. MEDLINE/Pubmed and Web of Science databases were accessed for searching studies measuring the STS task directly; identification, objective, design, sample, protocols and results data were extracted; the risk of bias was assessed (PROSPERO CRD42017055693). From 13,155 studies, 37 were included, and all demonstrated a low to moderate risk of bias. The STS task was applied in all world, but the protocols varied across studies, and they lacked detail; robust evidence demonstrating the association between STS task and musculoskeletal fitness was found; there was limited research examining body weight status, physical activity and the STS task performance. In conclusion, the STS task seems to be a universal tool to track motor functional competence and musculoskeletal fitness throughout life for clinical or research purposes.
A Review: The Prospect of Inhaled Insulin Therapy via Vibrating Mesh Technology to Treat Diabetes
Authors: Cunningham Seán M. , Tanner David A.
Published: August 2020

Background: Inhaled insulin has proven to be viable and, in some aspects, a more effective alternative to subcutaneous insulin. Past and present insulin inhaler devices have not found clinical or commercial success. Insulin inhalers create a dry powder or soft mist insulin aerosol, which does not provide the required uniform particle size or aerosol volume for deep lung deposition. Methods: The primary focus of this review is to investigate the potential treatment of diabetes with a wet insulin aerosol. Vibrating mesh nebulisers allow the passive inhalation of a fine wet mist aerosol for the administration of drugs to the pulmonary system in higher volumes than other small-volume nebulisers. Results: At present, there is a significant focus on vibrating mesh nebulisers from the pharmaceutical and biomedical industries for the systemic administration of pharmaceuticals for non-traditional applications such as vaccines or the treatment of diabetes. Systemic drug administration using vibrating mesh nebulisers leads to faster-acting pharmaceuticals with a reduction in drug latency. Conclusions: Systemic conditions such as diabetes, require the innovative development of custom vibrating mesh devices to provide the desired flow rates and droplet size for effective inhaled insulin administration.
Navigating Life with HIV as an Older Adult in South African Communities: A Phenomenological Study
Authors: Hlongwane Naomi , Madiba Sphiwe
Published: August 2020

The study explored how older adults experience an HIV diagnosis, deal with issues of stigma and disclosure, and navigate the healthcare system. Descriptive phenomenology was used to collect data from 20 older adults receiving antiretroviral treatment in health facilities in Gauteng Province, South Africa. Data analysis was inductive and followed the thematic approach. After diagnosis with HIV, the older adults experienced shock and disbelief, internalized their new reality of being HIV-positive, and found it difficult to disclose their HIV status. Stigma was embedded in their patterns of disclosure, and they chose not to disclose in order to manage stigma, protect their status, and maintain their privacy. Moreover, nondisclosure allowed them to achieve some normality in their lives without the fear of rejection. The older adults adopted various survival skills that aided them to accept their HIV status. Self-acceptance was important for the older adults who did not want to feel cheated out of life by the HIV diagnosis. The positive supportive attitudes of the healthcare professionals provided the much-needed support network for the older adults immediately after they had received their HIV test results. This was instrumental in their acceptance of their HIV status, their adherence to clinic visits, and their ability to live a healthy, positive life.
The Difficulties and Needs of Organ Transplant Recipients during Postoperative Care at Home: A Systematic Review
Authors: Yang Fu-Chi , Chen Hsiao-Mei , Huang Chiu-Mieh , Hsieh Pei-Lun , Wang Shoei-Shen , Chen Ching-Min
Published: August 2020

With recent advances in surgery and immunosuppressive drugs, organ transplantation has become a major treatment for irreversible organ failure. However, organ transplant recipients returning home after operation may face ongoing physiological, psychological, and social difficulties. To increase recipients’ quality of life, postoperative care at home is critical. Thus, the aim of this systematic literature review was to explore recipients’ difficulties and needs during postoperative care at home. Our search conformed to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and returned 23 relevant articles published from 1997–2020 in PubMed, MEDLINE, EBSCO, Cochrane, ProQuest, and CEPS, which were assessed using the Modified Jadad Scale or the 32 Consolidated Criteria for Reporting Qualitative Research (COREQ) appraisal indices and then synthesized through narration. The most common difficulties faced were psychological difficulties, followed by physiological, social, and other difficulties; the most common needs were psychological needs, followed by education and information training, social, and other needs. These results demonstrated that healthcare professionals can do more to provide patients with comprehensive care and promote successful self-management and quality of life at home. They also confirmed that collaboration between transplant teams, caregivers, and patients is necessary to optimize postoperative outcomes. We suggest that customized care may promote postoperative patients’ self-management and quality of life at home.
Clinical and Regulatory Concerns of Biosimilars: A Review of Literature
Authors: Halimi Vesa , Daci Armond , Ancevska Netkovska Katerina , Suturkova Ljubica , Babar Zaheer-Ud-Din , Grozdanova Aleksandra
Published: August 2020

Although biosimilars have been part of clinical practice for more than a decade, healthcare professionals (HCPs) do not fully accept them. This is because of the perception that biosimilars may not be like their originators in terms of quality, safety, and efficacy. This study aims to evaluate the current knowledge and attitudes of healthcare professionals toward biosimilar prescription, and to elaborate on their concerns. We reviewed the literature using PubMed, Cochrane Library, and Science Direct electronic databases in the period from 2018 to 2020. The knowledge and confidence of healthcare professionals vary between countries, between clinical profiles and between studies. Although most of the healthcare professionals had a positive attitude to prescribing biosimilars, they would still prefer to prescribe them in initial treatment. Generally, HCPs were against multiple switches and substitution of biosimilars at the pharmacy level. HCP’s key concern was interchangeability, with eventual consequences on the clinical outcome of patients. HCPs still approach biosimilars with caution and stigma. HCPs need to have an unbiased coherent understanding of biosimilars at clinical, molecular and regulatory levels. It was also observed that most of their concerns are more theoretical than science-based. Physicians are in an excellent position to accept biosimilars, but they need the additional support of regulatory authorities to approve and take into consideration the available scientific data regarding biosimilars.
Household Air Pollution and Angina Pectoris in Low- and Middle-Income Countries: Cross-Sectional Evidence from the World Health Survey 2002–2003
Authors: Tiwari Ishwar , Herr Raphael M. , Loerbroks Adrian , Yamamoto Shelby S.
Published: August 2020

The evidence regarding the effects of household air pollution on angina pectoris is limited in low-and middle-income countries (LMICs). We sought to examine the association between household air pollution and angina pectoris across several countries. We analyzed data of individuals from 46 selected countries participating in the cross-sectional World Health Survey (WHS) 2002–2003. Pooled and stratified (sex, continent) logistic regression with sampling weights was used to estimate adjusted odds ratios (ORs) and 95% confidence intervals (CIs) to quantify associations between the use of different household fuels with angina pectoris. In the pooled sample, we observed lower odds of angina pectoris with electricity use (OR: 0.68, 95% CI: 0.56–0.83) compared to those households reporting the use of gas as a household fuel. Increased odds of angina pectoris were observed with the use of agriculture/dung/shrub/other (OR: 1.65, 95% CI: 1.30–2.09), mixed (solid and non-solid fuels) (OR: 1.31, 95% CI: 1.09–1.56), and mixed solid fuel use (OR: 1.59, 95% CI: 1.12–2.25). Higher odds of angina pectoris were observed mainly with solid fuel use. The results highlight the importance of addressing these issues, especially in regions with a high proportion of solid fuel users and increasing levels of cardiovascular disease.
Implementation of the ‘Optimising the Health Extension Program’ Intervention in Ethiopia: A Process Evaluation Using Mixed Methods
Authors: Okwaraji Yemisrach B. , Hill Zelee , Defar Atkure , Berhanu Della , Wolassa Desta , Persson Lars Åke , Gonfa Geremew , Schellenberg Joanna A.
Published: August 2020

An intervention called ‘Optimising the Health Extension Program’, aiming to increase care-seeking for childhood illnesses in four regions of Ethiopia, was implemented between 2016 and 2018, and it included community engagement, capacity building, and district ownership and accountability. A pragmatic trial comparing 26 districts that received the intervention with 26 districts that did not found no evidence to suggest that the intervention increased utilisation of services. Here we used mixed methods to explore how the intervention was implemented. A fidelity analysis of each 31 intervention activities was performed, separately for the first phase and for the entire implementation period, to assess the extent to which what was planned was carried out. Qualitative interviews were undertaken with 39 implementers, to explore the successes and challenges of the implementation, and were analysed by using thematic analysis. Our findings show that the implementation was delayed, with only 19% (n = 6/31) activities having high fidelity in the first phase. Key challenges that presented barriers to timely implementation included the following: complexity both of the intervention itself and of administrative systems; inconsistent support from district health offices, partly due to competing priorities, such as the management of disease outbreaks; and infrequent supervision of health extension workers at the grassroots level. We conclude that, for sustainability, evidence-based interventions must be aligned with national health priorities and delivered within an existing health system. Strategies to overcome the resulting complexity include a realistic time frame and investment in district health teams, to support implementation at grassroots level.
Estimated 24 h Urinary Sodium-to-Potassium Ratio Is Related to Renal Function Decline: A 6-Year Cohort Study of Japanese Urban Residents
Authors: Hattori Hiroko , Hirata Aya , Kubo Sachimi , Nishida Yoko , Nozawa Miki , Kawamura Kuniko , Hirata Takumi , Kubota Yoshimi , Sata Mizuki , Kuwabara Kazuyo , Higashiyama Aya , Kadota Aya , Sugiyama Daisuke , Miyamatsu Naomi , Miyamoto Yoshihiro , Okamura Tomonori
Published: August 2020

The effect of the sodium-to-potassium ratio (Na/K) on renal function within the clinically normal range of renal function are limited. We investigated the effects of an estimated 24 h urinary Na/K (e24hUNa/K) on a 6-year renal function decline among 927 urban Japanese community dwellers with no history of cardiovascular diseases and medication for hypertension, diabetes, or dyslipidemia. We partitioned the subjects into quartiles according to the e24hUNa/K. The estimated glomerular filtration rate (eGFR) was calculated using the chronic kidney disease epidemiology collaboration (CKD/EPI) formula and renal function decline was defined as an absolute value at or above the third quartile of the eGFR decline rate. A multivariable logistic regression model was used for estimation. Compared with the first quartile of the e24hUNa/K, multivariable-adjusted odds ratios (ORs) for eGFR decline in the second, third, and fourth quartiles were 0.96 (95% confidence interval: 0.61–1.51), 1.06 (0.67–1.66), and 1.65 (1.06–2.57), respectively. These results were similar when the simple spot urine Na/K ratio was used in place of the e24hUNa/K. Apparently healthy urban residents with an almost within normal range mean baseline eGFR and high e24hUNa/K ratios had an increased risk for a future decline in renal function. Reducing the Na/K ratio may be important in the prevention of chronic kidney disease in its early stage.
Zero-Valent Iron Nanoparticles for Soil and Groundwater Remediation
Authors: Galdames Alazne , Ruiz-Rubio Leire , Orueta Maider , Sánchez-Arzalluz Miguel , Vilas-Vilela José Luis
Published: August 2020

Zero-valent iron has been reported as a successful remediation agent for environmental issues, being extensively used in soil and groundwater remediation. The use of zero-valent nanoparticles have been arisen as a highly effective method due to the high specific surface area of zero-valent nanoparticles. Then, the development of nanosized materials in general, and the improvement of the properties of the nano-iron in particular, has facilitated their application in remediation technologies. As the result, highly efficient and versatile nanomaterials have been obtained. Among the possible nanoparticle systems, the reactivity and availability of zero-valent iron nanoparticles (NZVI) have achieved very interesting and promising results make them particularly attractive for the remediation of subsurface contaminants. In fact, a large number of laboratory and pilot studies have reported the high effectiveness of these NZVI-based technologies for the remediation of groundwater and contaminated soils. Although the results are often based on a limited contaminant target, there is a large gap between the amount of contaminants tested with NZVI at the laboratory level and those remediated at the pilot and field level. In this review, the main zero-valent iron nanoparticles and their remediation capacity are summarized, in addition to the pilot and land scale studies reported until date for each kind of nanomaterials.
Analysis of Wellbeing in Nongovernmental Organizations’ Workplace in a Developed Area Context
Authors: Navajas-Romero Virginia , Caridad y López del Río Lorena , Ceular-Villamandos Nuria
Published: August 2020

An extremely useful theoretical approach to understanding the nature of work, health, and wellbeing is the job demand–control (JDC) model and the job demand–control–support (JDCS) model. In order for professional workers in the nongovernmental organization (NGO) sector to do their job, it is necessary for them to have a feeling of wellbeing. Despite this, in Europe, studies regarding the effects of the JDCS model in relation to workers’ wellbeing have not been carried out. This study is expected to fill this important gap in research by analyzing the relationship of wellbeing with work demands, work control, and social support. In order to corroborate the proposed hypotheses, an analysis of these constructs in employees in European nongovernmental organizations (NGOs) was developed and, using structural equation models, these relationships were tested. The results confirm the main hypothesis of the job demand–control–support (JDCS) model and the causal relationship among physical and psychological demands, work control, and support from supervisors and colleagues with the level of employee wellbeing.
Implementing Mental Health Promotion Initiatives—Process Evaluation of the ABCs of Mental Health in Denmark
Authors: Hinrichsen Carsten , Koushede Vibeke Jenny , Madsen Katrine Rich , Nielsen Line , Ahlmark Nanna Gram , Santini Ziggi Ivan , Meilstrup Charlotte
Published: August 2020

Treatment and prevention alone are unlikely to make a significant difference in reducing the burden of poor mental health and mental illness. Therefore, mental health promotion (MHP) initiatives are advocated. In 2014, the ABCs of mental health (ABCs) partnership was established in Denmark; in the partnership, partner organisations, e.g., municipalities and NGOs, use a research-based framework for MHP, the ABC-framework, to develop and implement MHP initiatives. This paper has two aims: (1) to outline the overall characteristics of these MHP initiatives; and (2) to explore local coordinator and stakeholder perceptions of the implementation processes and the impact of the MHP initiatives. Questionnaire surveys, individual interviews and group interviews were conducted during 2017–2020. The MHP initiatives were grouped according to three strategies: building MHP capacity, campaign activities to promote mental health awareness and knowledge and establishing and promoting opportunities to engage in mentally healthy activities. The ABC-framework was positively received and viewed as providing relevant knowledge for working with MHP as well as fostering intersectoral and interprofessional collaborations. However, using a bottom-up approach to develop and implement MHP initiatives can be time-consuming and resource demanding, and it requires a deliberate balancing of local adaptability and concrete guidance when engaging stakeholders and implementers. Overall, using the ABC-framework to develop and implement MHP initiatives holds great promise for advancing and promoting MHP practice.
Changes in Sex Life among People in Taiwan during the COVID-19 Pandemic: The Roles of Risk Perception, General Anxiety, and Demographic Characteristics
Authors: Ko Nai-Ying , Lu Wei-Hsin , Chen Yi-Lung , Li Dian-Jeng , Chang Yu-Ping , Wu Chia-Fen , Wang Peng-Wei , Yen Cheng-Fang
Published: August 2020

This study used data collected from an online survey study on coronavirus disease 2019 (COVID-19) in Taiwan to examine changes in sex life during the pandemic and the factors affecting such changes. In total, 1954 respondents were recruited from a Facebook advertisement. The survey inquired changes in sex life during the pandemic, including satisfaction with the individual’s sex life, frequency of sexual activity, frequency of sex-seeking activity, and frequency of using protection for sex. The associations of change in sex life with risk perception of COVID-19, general anxiety, gender, age, and sexual orientation were also examined. For each aspect of their sex life, 1.4%–13.5% of respondents reported a decrease in frequency or satisfaction, and 1.6%–2.9% reported an increase in frequency or satisfaction. Risk perception of COVID-19 was significantly and negatively associated with frequencies of sexual and sex-seeking activities. Higher general anxiety was significantly and negatively associated with satisfaction of sex life and frequencies of sexual and sex-seeking activities. Sexual minority respondents were more likely to report decreased satisfaction with sex life and frequencies of sexual activity and sex-seeking activities during COVID-19. Health care providers should consider these factors when developing strategies for sexual wellness amid respiratory infection epidemics.
Financial Risk and Financial Performance: Evidence and Insights from Commercial and Services Listed Companies in Nairobi Securities Exchange, Kenya
Authors: Onsongo Susan Kerubo , Muathe Stephen M. A. , Mwangi Lucy Wamugo
Published: August 2020

In Kenya, the last few years has seen the performance of companies listed under the commercial and services segment on the Nairobi Securities Exchange (NSE), experience mixed fortunes. The study sought to assess the implications of financial risk on the performance of these companies. The study applied explanatory research design. The target population were the 14 companies listed under this segment of NSE. Secondary panel data contained in published annual reports for the period 2013–2017 was collected. Panel regression model was applied with the random effect model being used based on the Hausman specification test. Findings showed that credit risk had an insignificant positive effect on return on equity (ROE) while liquidity risk had a significantly negative effect on ROE and operational risk had a positive insignificant effect on ROE. The positive coefficients from the data analysis indicated that commercial and service companies at NSE were able to take in more credit to boost performance of these companies however the negative coefficients shows that within the period of study these companies experienced high liquidity problems in that the current liabilities exceeded the current assets. Thus, concluding that these companies were unable to pay all their obligation when they were due.
Spatiotemporal Assessment of Irrigation Performance of the Kou Valley Irrigation Scheme in Burkina Faso Using Satellite Remote Sensing-Derived Indicators
Authors: Sawadogo Alidou , Kouadio Louis , Traoré Farid , Zwart Sander J. , Hessels Tim , Gündoğdu Kemal Sulhi
Published: August 2020

Traditional methods based on field campaigns are generally used to assess the performance of irrigation schemes in Burkina Faso, resulting in labor-intensive, time-consuming, and costly processes. Despite their extensive application for such performance assessment, remote sensing (RS)-based approaches remain very much underutilized in Burkina Faso. Using multi-temporal Landsat images within the Python module for the Surface Energy Balance Algorithm for Land model, we investigated the spatiotemporal performance patterns of the Kou Valley irrigation scheme (KVIS) during two consecutive cropping seasons. Four performance indicators (depleted fraction, relative evapotranspiration, uniformity of water consumption, and crop water productivity) for rice, maize, and sweet potato were calculated and compared against standard values. Overall, the performance of the KVIS varied depending on year, crop, and the crop’s geographical position in the irrigation scheme. A gradient of spatially varied relative evapotranspiration was observed across the scheme, with the uniformity of water consumption being fair to good. Although rice was the most cultivated, a shift to more sweet potato farming could be adopted to benefit more from irrigation, given the relatively good performance achieved by this crop. Our findings ascertain the potential of such RS-based cost-effective methodologies to serve as basis for improved irrigation water management in decision support tools.
A Subject-Sensitive Perceptual Hash Based on MUM-Net for the Integrity Authentication of High Resolution Remote Sensing Images
Authors: Ding Kaimeng , Liu Yueming , Xu Qin , Lu Fuqiang
Published: August 2020

Data security technology is of great significance to the application of high resolution remote sensing image (HRRS) images. As an important data security technology, perceptual hash overcomes the shortcomings of cryptographic hashing that is not robust and can achieve integrity authentication of HRRS images based on perceptual content. However, the existing perceptual hash does not take into account whether the user focuses on certain types of information of the HRRS image. In this paper, we introduce the concept of subject-sensitive perceptual hash, which can be seen as a special case of conventional perceptual hash, for the integrity authentication of HRRS image. To achieve subject-sensitive perceptual hash, we propose a new deep convolutional neural network architecture, named MUM-Net, for extracting robust features of HRRS images. MUM-Net is the core of perceptual hash algorithm, and it uses focal loss as the loss function to overcome the imbalance between the positive and negative samples in the training samples. The robust features extracted by MUM-Net are further compressed and encoded to obtain the perceptual hash sequence of HRRS image. Experiments show that our algorithm has higher tamper sensitivity to subject-related malicious tampering, and the robustness is improved by about 10% compared to the existing U-net-based algorithm; compared to other deep learning-based algorithms, this algorithm achieves a better balance between robustness and tampering sensitivity, and has better overall performance.
Capsaicin-Cyclodextrin Complex Enhances Mepivacaine Targeting and Improves Local Anesthesia in Inflamed Tissues
Authors: Couto Verônica Muniz , de Oliveira-Nascimento Laura , Cabeça Luiz Fernando , Geraldes Danilo Costa , Costa Juliana Souza Ribeiro , Riske Karin A. , Franz-Montan Michelle , Yokaychiya Fabiano , Franco Margareth K. K. Dias , de Paula Eneida
Published: August 2020

Acidic environments, such as in inflamed tissues, favor the charged form of local anesthetics (LA). Hence, these drugs show less cell permeation and diminished potency. Since the analgesic capsaicin (CAP) triggers opening of the TRPV1 receptor pore, its combination with LAs could result in better uptake and improved anesthesia. We tested the above hypothesis and report here for the first time the analgesia effect of a two-drug combination (LA and CAP) on an inflamed tissue. First, CAP solubility increased up to 20 times with hydroxypropyl-beta-cyclodextrin (HP-β-CD), as shown by the phase solubility study. The resulting complex (HP-β-CD-CAP) showed 1:1 stoichiometry and high association constant, according to phase-solubility diagrams and isothermal titration calorimetry data. The inclusion complex formation was also confirmed and characterized by differential scanning calorimetry (DSC), X-ray diffraction, and 1H-NMR. The freeze-dried complex showed physicochemical stability for at least 12 months. To test in vivo performance, we used a pain model based on mouse paw edema. Results showed that 2% mepivacaine injection failed to anesthetize mice inflamed paw, but its combination with complexed CAP resulted in pain control up to 45 min. These promising results encourages deeper research of CAP as an adjuvant for anesthesia in inflamed tissues and cyclodextrin as a solubilizing agent for targeting molecules in drug delivery.
Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer
Authors: Krstić Ristivojević Maja , Grundström Jeanette , Apostolović Danijela , Radomirović Mirjana , Jovanović Vesna , Radoi Vlad , Kiewiet M. B. Gea , Vukojević Vladana , Ćirković Veličković Tanja , van Hage Marianne
Published: August 2020

Transepithelial transport of proteins is an important step in the immune response to food allergens. Mammalian meat allergy is characterized by an IgE response against the carbohydrate moiety galactosyl-α-1,3-galactose (α-Gal) present on mammalian glycoproteins and glycolipids, which causes severe allergic reactions several hours after red meat consumption. The delayed reaction may be related to the processing of α-Gal carrying proteins in the gastrointestinal tract. The aim of this study was to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer. We found that α-Gal glycosylation altered protein susceptibility to gastric digestion, where large protein fragments bearing the α-Gal epitope remained for up to 2 h of digestion. Furthermore, α-Gal glycosylation of the protein hampered transcytosis of the protein through the Caco-2 monolayer. α-Gal epitope on the intact protein could be detected in the endosomal fraction obtained by differential centrifugation of Caco-2 cell lysates. Furthermore, the level of galectin-3 in Caco-2 cells was not affected by the presence of α-Gal glycosylated BSA (bovine serum albumin) (BSA-α-Gal). Taken together, our data add new knowledge and shed light on the digestion and transport of α-Gal glycosylated proteins.
Premature Activation of Immune Transcription Programs in Autoimmune-Predisposed Mouse Embryonic Stem Cells and Blastocysts
Authors: Kirak Oktay , Ke Eugene , Yang Kevin Y. , Schwarz Anna , Plate Lars , Nham Amy , Abadejos Justin R. , Valencia Anna , Wiseman R. Luke , Lui Kathy O. , Ku Manching
Published: August 2020

Autoimmune diabetes is a complex multifactorial disease with genetic and environmental factors playing pivotal roles. While many genes associated with the risk of diabetes have been identified to date, the mechanisms by which external triggers contribute to the genetic predisposition remain unclear. Here, we derived embryonic stem (ES) cell lines from diabetes-prone non-obese diabetic (NOD) and healthy C57BL/6 (B6) mice. While overall pluripotency markers were indistinguishable between newly derived NOD and B6 ES cells, we discovered several differentially expressed genes that normally are not expressed in ES cells. Several genes that reside in previously identified insulin-dependent diabetics (Idd) genomic regions were up-regulated in NOD ES cells. Gene set enrichment analysis showed that different groups of genes associated with immune functions are differentially expressed in NOD. Transcriptomic analysis of NOD blastocysts validated several differentially overexpressed Idd genes compared to B6. Genome-wide mapping of active histone modifications using ChIP-Seq supports active expression as the promoters and enhancers of activated genes are also marked by active histone modifications. We have also found that NOD ES cells secrete more inflammatory cytokines. Our data suggest that the known genetic predisposition of NOD to autoimmune diabetes leads to epigenetic instability of several Idd regions.
Towards the Development of AgoKirs: New Pharmacological Activators to Study Kir2.x Channel and Target Cardiac Disease
Authors: van der Schoor Laura , van Hattum Emma J. , de Wilde Sophie M. , Harlianto Netanja I. , van Weert Aart-Jan , Bloothooft Meye , van der Heyden Marcel A. G.
Published: August 2020

Inward rectifier potassium ion channels (IK1-channels) of the Kir2.x family are responsible for maintaining a stable negative resting membrane potential in excitable cells, but also play a role in processes of non-excitable tissues, such as bone development. IK1-channel loss-of-function, either congenital or acquired, has been associated with cardiac disease. Currently, basic research and specific treatment are hindered by the absence of specific and efficient Kir2.x channel activators. However, twelve different compounds, including approved drugs, show off-target IK1 activation. Therefore, these compounds contain valuable information towards the development of agonists of Kir channels, AgoKirs. We reviewed the mechanism of IK1 channel activation of these compounds, which can be classified as direct or indirect activators. Subsequently, we examined the most viable starting points for rationalized drug development and possible safety concerns with emphasis on cardiac and skeletal muscle adverse effects of AgoKirs. Finally, the potential value of AgoKirs is discussed in view of the current clinical applications of potentiators and activators in cystic fibrosis therapy.
In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
Authors: Clara-Trujillo Sandra , Gallego Ferrer Gloria , Gómez Ribelles José Luis
Published: August 2020

In hematological malignancies, leukemias or myelomas, malignant cells present bone marrow (BM) homing, in which the niche contributes to tumor development and drug resistance. BM architecture, cellular and molecular composition and interactions define differential microenvironments that govern cell fate under physiological and pathological conditions and serve as a reference for the native biological landscape to be replicated in engineered platforms attempting to reproduce blood cancer behavior. This review summarizes the different models used to efficiently reproduce certain aspects of BM in vitro; however, they still lack the complexity of this tissue, which is relevant for fundamental aspects such as drug resistance development in multiple myeloma. Extracellular matrix composition, material topography, vascularization, cellular composition or stemness vs. differentiation balance are discussed as variables that could be rationally defined in tissue engineering approaches for achieving more relevant in vitro models. Fully humanized platforms closely resembling natural interactions still remain challenging and the question of to what extent accurate tissue complexity reproduction is essential to reliably predict drug responses is controversial. However, the contributions of these approaches to the fundamental knowledge of non-solid tumor biology, its regulation by niches, and the advance of personalized medicine are unquestionable.
A Fungal Ascorbate Oxidase with Unexpected Laccase Activity
Authors: Braunschmid Verena , Fuerst Sarah , Perz Veronika , Zitzenbacher Sabine , Hoyo Javier , Fernandez-Sanchez Cesar , Tzanov Tzanko , Steinkellner Georg , Gruber Karl , Nyanhongo Gibson S. , Ribitsch Doris , Guebitz Georg M.
Published: August 2020

Ascorbate oxidases are an enzyme group that has not been explored to a large extent. So far, mainly ascorbate oxidases from plants and only a few from fungi have been described. Although ascorbate oxidases belong to the well-studied enzyme family of multi-copper oxidases, their function is still unclear. In this study, Af_AO1, an enzyme from the fungus Aspergillus flavus, was characterized. Sequence analyses and copper content determination demonstrated Af_AO1 to belong to the multi-copper oxidase family. Biochemical characterization and 3D-modeling revealed a similarity to ascorbate oxidases, but also to laccases. Af_AO1 had a 10-fold higher affinity to ascorbic acid (KM = 0.16 ± 0.03 mM) than to ABTS (KM = 1.89 ± 0.12 mM). Furthermore, the best fitting 3D-model was based on the ascorbate oxidase from Cucurbita pepo var. melopepo. The laccase-like activity of Af_AO1 on ABTS (Vmax = 11.56 ± 0.15 µM/min/mg) was, however, not negligible. On the other hand, other typical laccase substrates, such as syringaldezine and guaiacol, were not oxidized by Af_AO1. According to the biochemical and structural characterization, Af_AO1 was classified as ascorbate oxidase with unusual, laccase-like activity.