bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Factors of Pneumonia Associated with Mechanical Ventilation
Authors: Kózka Maria , Sega Aurelia , Wojnar-Gruszka Katarzyna , Tarnawska Agnieszka , Gniadek Agnieszka
Published: January 2020

Background: The hospitalization of patients treated in the intensive care unit (ICU) in 5–15% of cases is associated with the occurrence of a complication in the form of ventilator-associated pneumonia (VAP). Purpose: Retrospective assessment of risk factors of VAP in patients treated at ICUs in the University Hospital in Krakow. Methods: The research involved the medical documentation of 1872 patients treated at the ICU of the University Hospital in Krakow between 2014 and 2017. The patients were mechanically ventilated for at least 48 h. The obtained data were presented by qualitative and quantitative analysis (%). The qualitative variables were compared using the Chi2 test. Statistically significant was the p < 0.05 value. Results: VAP was demonstrated in 23% of all patients treated in ICU during the analyzed period, and this infection occurred in 13% of men and 10% of women. Pneumonia associated with ventilation was found primarily in patients staying in the ward for over 15 days and subjected to intratracheal intubation (17%). A statistically significant was found between VAP and co-morbidities, e.g., chronic obstructive pulmonary disease, diabetes, alcoholism, obesity, the occurrence of VAP and multi-organ trauma, hemorrhage/hemorrhagic shock, and fractures as the reasons for admitting ICU patients. Conclusions: Patients with comorbidities such as chronic obstructive pulmonary disease, obesity, diabetes, and alcoholism are a high-risk group for VAP. Particular attention should be paid to patients admitted to the ICU with multi-organ trauma, fractures, and hemorrhage/hemorrhagic shock as patients predisposed to VAP. There is a need for further research into risk factors for non-modifiable VAP such as comorbidities and reasons for ICU admission in order to allow closer monitoring of these patients for VAP.
Exploring Tiny Homes as an Affordable Housing Strategy to Ameliorate Homelessness: A Case Study of the Dwellings in Tallahassee, FL
Authors: Jackson April , Callea Bridget , Stampar Nicholas , Sanders Abigail , De Los Rios Alberto , Pierce Jake
Published: January 2020

An emerging strategy to combat homelessness is the development of “tiny homes”. However, the advent of tiny homes as a new form of housing intervention raises a number of questions about their intentions, efficacy, and policy feasibility. This paper seeks to understand the strategies used by stakeholders to plan, design, and implement a tiny home community to meet the needs of those experiencing homelessness, and to understand where these plans were effective and where challenges arose in meeting the intended project goals. Utilizing the recent development of Tallahassee’s “The Dwellings” project as a case study, we examine how the community was planned, resident experiences, and constraints to implementing a tiny home development. We use qualitative methods that include interviews with stakeholders who were integral to the planning and development process. Our findings highlight how funding constraints and NIMBYism (Not in My Backyard-ism) stymied stakeholder efforts to achieve equity and affordability at The Dwellings, resulting in the inability to achieve project aims of developing housing that serves the homeless population. We conclude by offering some observations and lessons learned for future research on tiny homes as a solution to ameliorate homelessness.
Data Model for IndoorGML Extension to Support Indoor Navigation of People with Mobility Disabilities
Authors: Park Seula , Yu Kiyun , Kim Jiyoung
Published: January 2020

The increasing complexity of modern buildings has challenged the mobility of people with disabilities (PWD) in the indoor environment. To help overcome this problem, this paper proposes a data model that can be easily applied to indoor spatial information services for people with disabilities. In the proposed model, features are defined based on relevant regulations that stipulate significant mobility factors for people with disabilities. To validate the model’s capability to describe the indoor spaces in terms that are relevant to people with mobility disabilities, the model was used to generate data in a path planning application, considering two different cases in a shopping mall. The application confirmed that routes for people with mobility disabilities are significantly different from those of ordinary pedestrians, in a way that reflects features and attributes defined in the proposed data model. The latter can be inserted as an IndoorGML extension, and is thus expected to facilitate relevant data generation for the design of various services for people with disabilities.
Multitemporal Land Use and Land Cover Classification from Time-Series Landsat Datasets Using Harmonic Analysis with a Minimum Spectral Distance Algorithm
Authors: Sun Jing , Ongsomwang Suwit
Published: January 2020

An understanding of historical and present land use and land cover (LULC) information and its changes, such as urbanization and urban growth, is critical for city planners, land managers and resource managers in any rapidly changing landscape. To deal with this situation, the development of a new supervised classification method for multitemporal LULC mapping with long-term reliable information is necessary. The ultimate goal of this study was to develop a new classification method using harmonic analysis with a minimum spectral distance algorithm for multitemporal LULC mapping. Here, the Jiangning District of Nanjing City, Jiangsu Province, China was chosen as the study area. The research methodology consisted of two main components: (1) Landsat data selection and time-series spectral reflectance reconstruction and (2) multitemporal LULC classification using HA with a minimum spectral distance algorithm. The results revealed that the overall accuracy and Kappa hat coefficients of the four LULC maps in 2000, 2006, 2011, and 2017 were 97.03%, 90.25%, 91.19%, 86.32% and 95.35%, 84.48%, 86.74%, 80.24%, respectively. Further, the average producer accuracy and user accuracy of the urban and built-up land, agricultural land, forest land, and water bodies from the four LULC maps were 92.30%, 90.98%, 94.80%, 85.65% and 90.28%, 93.17%, 84.40%, 99.50%, respectively. Consequently, it can be concluded that the newly developed supervised classification method using harmonic analysis with a minimum spectral distance algorithm can efficiently classify multitemporal LULC maps.
Resistance Assessment of Service-Proven Aging Bridges Incorporating Deterioration-Load Dependency
Authors: Wang Cao
Published: January 2020

The historical vehicles passed through an existing bridge can be regarded as proof-loading tests for the bridge, and, as a result, are evident of the bridge’s performance. Such service history information has been utilized to update the estimate of bridge resistance in previous studies with the help of a Bayesian method, where the resistance deterioration process was assumed to be independent of the vehicle load process. This assumption is, however, untenable in many cases where the deterioration stochastic process is statistically correlated with the load process (e.g., a greater load intensity may affect/accelerate the deterioration of structural resistance and the accumulation of structural fragility). With this regard, this paper investigates the effect of correlation between the resistance deterioration and load processes on updating the resistance of aging bridges with prior service load information. The copula function is employed to model the joint distribution of the correlated deterioration and load processes, with which the correlation is measured by the Kendall’s tau. A new method is developed in this paper to assess the updated bridge resistance taking into consideration the deterioration-load dependency in an explicit form. The applicability of the proposed method is illustrated using an existing RC beam bridge. The sensitivity analysis is conducted to examine how the deterioration-load dependency affects the updated resistance of service-proven aging bridges.
Acknowledgement to Reviewers of Insects in 2019
Authors: Insects Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not [...]
Pregnancy Associated Plasma Protein-A as a Cardiovascular Risk Marker in Patients with Stable Coronary Heart Disease During 10 Years Follow-Up—A CLARICOR Trial Sub-Study
Authors: Nilsson Erik , Kastrup Jens , Sajadieh Ahmad , Boje Jensen Gorm , Kjøller Erik , Kolmos Hans Jørn , Wuopio Jonas , Nowak Christoph , Larsson Anders , Jakobsen Janus Christian , Winkel Per , Gluud Christian , Iversen Kasper K , Ärnlöv Johan , Carlsson Axel C.
Published: January 2020

Elevated pregnancy-associated plasma protein A (PAPP-A) is associated with mortality in acute coronary syndromes. Few studies have assessed PAPP-A in stable coronary artery disease (CAD) and results are conflicting. We assessed the 10-year prognostic relevance of PAPP-A levels in stable CAD. The CLARICOR trial was a randomized controlled clinical trial including outpatients with stable CAD, randomized to clarithromycin versus placebo. The placebo group constituted our discovery cohort (n = 1.996) and the clarithromycin group the replication cohort (n = 1.975). The composite primary outcome was first occurrence of cardiovascular event or death. In the discovery cohort, incidence rates (IR) for the composite outcome were higher in those with elevated PAPP-A (IR 12.72, 95% Confidence Interval (CI) 11.0–14.7 events/100 years) compared to lower PAPP-A (IR 8.78, 8.25–9.34), with comparable results in the replication cohort. Elevated PAPP-A was associated with increased risk of the composite outcome in both cohorts (discovery Hazard Ratio (HR) 1.45, 95% CI 1.24–1.70; replication HR 1.29, 95% CI 1.10–1.52). In models adjusted for established risk factors, these trends were attenuated. Elevated PAPP-A was associated with higher all-cause mortality in both cohorts. We conclude that elevated PAPP-A levels are associated with increased long-term mortality in stable CAD, but do not improve long-term prediction of death or cardiovascular events when added to established predictors.
Bone Marrow-Derived Mesenchymal Stromal Cells (MSCs) Modulate the Inflammatory Character of Alveolar Macrophages from Sarcoidosis Patients
Authors: McClain Caldwell Ian , Hogden Christopher , Nemeth Krisztian , Boyajian Michael , Krepuska Miklos , Szombath Gergely , MacDonald Sandra , Abshari Mehrnoosh , Moss Joel , Vitale-Cross Lynn , Fontana Joseph R , Mezey Eva
Published: January 2020

Sarcoidosis is a devastating inflammatory disease affecting many organs, especially the lungs and lymph nodes. Bone marrow-derived mesenchymal stromal cells (MSCs) can “reprogram” various types of macrophages towards an anti-inflammatory phenotype. We wanted to determine whether alveolar macrophages from sarcoidosis subjects behave similarly by mounting an anti-inflammatory response when co-cultured with MSCs. Fifteen sarcoidosis and eight control subjects underwent bronchoscopy and bronchoalveolar lavage (BAL). Unselected BAL cells (70–94% macrophages) were isolated and cultured with and without MSCs from healthy adults. Following stimulation of the cultured cells with lipopolysaccharide, the medium was removed to measure interleukin 10 and tumor necrosis factor alpha (IL-10 and TNF-α). In two additional sarcoidosis subjects, flow cytometry was used to study intracellular cytokines and surface markers associated with alveolar macrophages to confirm the results. Unselected BAL cells from sarcoidosis subjects co-cultured with MSCs showed a reduction in TNF-α (pro-inflammatory M1) and an increase in IL-10 (anti-inflammatory M2) in 9 of 11 samples studied. Control subject samples showed few, if any, differences in cytokine production. Unselected BAL cells from two additional patients analyzed by flow cytometry confirmed a switch towards an anti-inflammatory state (i.e., M1 to M2) after co-culture with MSCs. These results suggest that, similarly to other macrophages, alveolar macrophages also respond to MSC contacts by changing towards an anti-inflammatory phenotype. Based on our results, we hypothesize that mesenchymal stromal cells applied to the airways might alleviate lung inflammation and decrease steroid need in patients with sarcoidosis.
Performance of FRAX in Predicting Fractures in US Postmenopausal Women with Varied Race and Genetic Profiles
Authors: Wu Qing , Xiao Xiangxue , Xu Yingke
Published: January 2020

Background: Whether the Fracture Risk Assessment Tool (FRAX) performed differently in estimating the 10-year fracture probability in women of different genetic profiling and race remained unclear. Methods: The genomic data in the Women’s Health Initiative (WHI) study was analyzed (n = 23,981). The genetic risk score (GRS) was calculated from 14 fracture-associated single nucleotide polymorphisms (SNPs) for each participant. FRAX without bone mineral density (BMD) was used to estimate fracture probability. Results: FRAX significantly overestimated the risk of major osteoporotic fracture (MOF) in the WHI study. The most significant overestimation was observed in women with low GRS (predicted/observed ratio (POR): 1.61, 95% CI: 1.45–1.79) specifically Asian women (POR: 3.5, 95% CI 2.48–4.81) and in African American women (POR: 2.59, 95% CI: 2.33–2.87). Compared to the low GRS group, the 10-year probability of MOF adjusted for the FRAX score was 21% and 30% higher in the median GRS group and high GRS group, respectively. Asian, African American, and Hispanic women respectively had a 78%, 76%, and 56% lower hazard than Caucasian women after the FRAX score was adjusted. The results were similar for hip fractures. Conclusions: Our study suggested the FRAX performance varies significantly by both genetic profile and race in postmenopausal women.
A Comparative Analysis of EEDI Versus Lifetime CO2 Emissions
Authors: Trivyza Nikoletta L. , Rentizelas Athanasios , Theotokatos Gerasimos
Published: January 2020

The Energy Efficiency Design Index (EEDI) was introduced as a regulatory tool employed at the ship design phase to reduce the carbon dioxide (CO2) emissions and increase the vessel’s operational efficiency. Although it stimulated the greening of the shipping operations, its effectiveness is considerably criticised from various shipping industry stakeholders. The aim of this study is to investigate the EEDI effectiveness on accurately representing the environmental performance of the next-generation ships power plants for two representative ship types, in specific, an ocean-going tanker and a cruise ship. The performance of the optimal power plant solutions identified in previous studies is analysed according to the existing EEDI regulatory framework and compared with the lifetime CO2 emissions estimated based on an actual operating profile for each ship. The results indicate that the EEDI underestimates the effect of technologies for reducing carbon emissions in all the investigated cases. In this respect, it is concluded that EEDI is classified as a conservative metric, which however can be used as an approximation to compare alternative solutions early in the design phase.
Effect of Variations in Water Level and Wave Steepness on the Robustness of Wave Overtopping Estimation
Authors: Kerpen Nils B. , Daemrich Karl-Friedrich , Lojek Oliver , Schlurmann Torsten
Published: January 2020

The wave overtopping discharge at coastal defense structures is directly linked to the freeboard height. By means of physical modelling, experiments on wave overtopping volumes at sloped coastal structures are customarily determined for constant water levels and static wave steepness conditions (e.g., specific wave spectrum). These experiments are the basis for the formulation of empirically derived and widely acknowledged wave overtopping estimations for practical design purposes. By analysis and laboratory reproduction of typical features from exemplarily regarded real storm surge time series in German coastal waters, the role of non-stationary water level and wave steepness were analyzed and adjusted in experiments. The robustness of wave overtopping estimation formulae (i.e., the capabilities and limitations of such a static projection of dynamic boundary conditions) are outlined. Therefore, the classic static approach is contrasted with data stemming from tests in which both water level and wave steepness were dynamically altered in representative arrangements. The analysis reveals that mean overtopping discharges for simple sloping structures in an almost deep water environment could be robustly estimated for dynamic water level changes by means of the present design formulae. In contrast, the role of dynamic changes of the wave steepness led to a substantial discrepancy of overtopping volumes by a factor of two. This finding opens new discussion on methodology and criteria design of coastal protection infrastructure under dynamic exposure to storm surges and in lieu of alterations stemming from projected sea level rise.
Breaching Flow Slides and the Associated Turbidity Current
Authors: Alhaddad Said , Labeur Robert Jan , Uijttewaal Wim
Published: January 2020

This paper starts with surveying the state-of-the-art knowledge of breaching flow slides, with an emphasis on the relevant fluid mechanics. The governing physical processes of breaching flow slides are explained. The paper highlights the important roles of the associated turbidity current and the frequent surficial slides in increasing the erosion rate of sediment. It also identifies the weaknesses of the current breaching erosion models. Then, the three-equation model of Parker et al. is utilised to describe the coupled processes of breaching and turbidity currents. For comparison’s sake, the existing breaching erosion models are considered: Breusers, Mastbergen and Van Den Berg, and Van Rhee. The sand erosion rate and hydrodynamics of the current vary substantially between the erosion models. Crucially, these erosion models do not account for the surficial slides, nor have they been validated due to the scarcity of data on the associated turbidity current. This paper motivates further experimental studies, including detailed flow measurements, to develop an advanced erosion model. This will improve the fidelity of numerical simulations.
Using a Multi-Layer Stacked AlGaN/GaN Structure to Improve the Current Spreading Performance of Ultraviolet Light-Emitting Diodes
Authors: Wang Yanli , Li Peixian , Zhang Xinyu , Xu Shengrui , Zhou Xiaowei , Wu Jinxing , Yue Wenkai , Hao Yue
Published: January 2020

To obtain excellent current spreading performance of ultraviolet light-emitting diodes (UVLEDs), a 60-period stacked Si modulation-doped n-AlGaN/u-GaN structure is proposed to replace the traditional n-AlGaN structure. The high-resolution X-ray diffraction ω-scan rocking curves show that the periodic growth of AlGaN and GaN layers plays a positive role in reducing dislocation density. Compared with the conventional UV light-emitting diodes (LEDs), light emission micrographs of devices with a multi-layer stacked n-AlGaN/u-GaN structure reveal higher brightness and a more uniform distribution. In addition, the output power and external quantum efficiency under a 20-mA injection current are increased by 22% and 26.5%, respectively. Experimental and simulation results indicate that a multi-layer stacking structure can alleviate the current crowding effect in four ways: (1) a reduction in dislocation density; (2) replacement of quasi-two-dimensional electron transport with electronic bulk transport to enhance electron mobility; (3) an increase in electron concentration without improving the impurity concentration; and (4) a weakening of the electron scattering effect by reducing the impurity concentration.
Fabrication and Tribological Properties of Mesocarbon Microbead–Cu Friction Composites
Authors: Guo Hai-Xia , Yang Jian-Feng
Published: January 2020

Graphite–metal composites have been used as friction materials owing to their self-lubricity, which is ascribed to the weak interlayer bonding of graphite. To overcome the shortage of graphite flake (GrF)-filled composites of having low tribological properties, graphite-Cu composites with mesocarbon microbead (MCMB) as the solid lubricant are developed in this paper. The MCMB–Cu composites have a lower friction coefficient and wear rate than do the GrF–Cu composites taken as reference materials, exhibiting a better self-lubricating performance. Microstructural analysis indicates that the relatively weaker interlayer bonding of the MCMB, smooth interface between the MCMB and matrix, and more cementite formation thorough reaction of MCMB and iron are the key factors behind the enhanced tribological properties. In addition, both the friction coefficients and wear rates of the two groups of composites gradually decrease with the graphite content. This work opens an avenue for designing desirable graphite-based metal friction materials.
Performance of 3D-Printed Continuous-Carbon-Fiber-Reinforced Plastics with Pressure
Authors: Zhang Jun , Zhou Zude , Zhang Fan , Tan Yuegang , Tu Yiwen , Yang Baojun
Published: January 2020

Fused Deposition Modeling (FDM) has been investigated as a low-cost manufacturing method for fiber-reinforced composites. The traditional and mature technology for manufacturing continuous-carbon-fiber-reinforced plastics is Automated Fiber Placement (AFP), which uses a consolidation roller and an autoclave process to improve the quality of parts. Compared to AFP, FDM is simple in design and operation but lacks the ability to pressurize and heat the model. In this work, a novel method for printing continuous carbon-fiber-reinforced plastics with a pressure roller was investigated. First, the path processing of the pressure roller was researched, which will reduce the number of rotations of the pressure roller and increase the service life of the equipment and the efficiency of printing. Thereafter, three specimens were printed under different pressures and the tensile and bending strength of specimens were tested. The tensile strength and bending strength of specimens were enhanced to 644.8 MPa and 401.24 MPa by increasing the pressure, compared to the tensile strength and bending strength of specimens without pressure of 109.9 MPa and 163.13 MPa. However, excessive pressure will destroy the path of the continuous carbon fiber (CCF) and the surface quality of the model, and may even lead to printing failure.
The Influence of Alternative Fillers on the Adhesive Properties of Mastics Fabricated with Red Mud
Authors: Lima Mayara S. Siverio , Thives Liseane P. , Haritonovs Viktors , Gschösser Florian
Published: January 2020

The adhesion between bitumen and aggregates strongly influences the lifetime of pavements. To improve adhesiveness, the road construction industry has been using additives to alter the interfacial energy and improve the affinity of materials in the presence of water. However, the water sensitivity varies according to the mixture design, since the interaction may occur differently depending on the materials chosen. As the use of alternative materials is increasing in road constructions, further analysis of its affinity with aggregates and bitumen is necessary. In that sense, this study evaluates the adhesion performance of mastics mixed with traditional fillers, such as limestone and dolomite, and residues, such as fly ash and red mud. To assess possible interactions with the red mud, the fillers are mixed in distinct percentages and tested for adhesiveness, wettability, penetration, and softening point. The results show the importance of hydrophilicity, asphalt viscosity, and physical–chemical properties to define adhesive interactions.
Magnetocaloric Effect in Cu5-NIPA Molecular Magnet: A Theoretical Study
Authors: Szałowski Karol , Kowalewska Pamela
Published: January 2020

We calculated the magnetocaloric properties of the molecular nanomagnet Cu5-NIPA, consisting of five spins S = 1 / 2 arranged in two corner-sharing triangles (hourglass-like structure without magnetic frustration). The thermodynamics of the system in question was described using the quantum Heisenberg model solved within the field ensemble (canonical ensemble) using exact numerical diagonalization. The dependence of the magnetic entropy and magnetic specific heat on the temperature and the external magnetic field was investigated. The isothermal entropy change for a wide range of initial and final magnetic fields was discussed. Due to plateau-like behavior of the isothermal entropy change as a function of the temperature, a high degree of tunability of magnetocaloric effect with the initial and final magnetic field was demonstrated.
A Lifting-Penalty Method for Quadratic Programming with a Quadratic Matrix Inequality Constraint
Authors: Liu Wei , Yang Li , Yu Bo
Published: January 2020

In this paper, a lifting-penalty method for solving the quadratic programming with a quadratic matrix inequality constraint is proposed. Additional variables are introduced to represent the quadratic terms. The quadratic programming is reformulated as a minimization problem having a linear objective function, linear conic constraints and a quadratic equality constraint. A majorization–minimization method is used to solve instead a l 1 penalty reformulation of the minimization problem. The subproblems arising in the method can be solved by using the current semidefinite programming software packages. Global convergence of the method is proven under some suitable assumptions. Some examples and numerical results are given to show that the proposed method is feasible and efficient.
Integrative Medicine in Interventional Oncology: A Virtuous Alliance
Authors: Cornelis François H. , Najdawi Milan , Ben Ammar Mohamed , Nouri-Neuville Maud , Lombart Bénédicte , Lotz Jean-Pierre , Cadranel Jacques , Barral Matthias
Published: January 2020

This review aimed to identify the potential role of integrative medicine in interventional oncology. The music therapy; stress management techniques; guided imagery, including virtual reality; clinical hypnosis; and digital sedation may all be efficient on anxiety and pain during procedures performed in interventional oncology. Beyond pharmacological sedation, the implementation of integrative medicine to interventional oncology may, therefore, improve the support and care of cancer patients, which may further create a virtuous alliance.
Pregnancy and Multiple Sclerosis: An Update on the Disease Modifying Treatment Strategy and a Review of Pregnancy’s Impact on Disease Activity
Authors: Varytė Guoda , Zakarevičienė Jolita , Ramašauskaitė Diana , Laužikienė Dalia , Arlauskienė Audronė
Published: January 2020

Pregnancy rates are rapidly increasing among women of reproductive age diagnosed with multiple sclerosis (MS). Through pre-conception, pregnancy and post-partum periods, there is a need for disease control management, to decrease chances of MS relapses while avoiding potential risks to the mother and the fetus. However, pregnancy is not always compatible with the available highly effective MS treatments. This narrative review provides the aspects of pregnancy’s outcomes and the impact on disease activity, choices of anesthesia and the management of relapses during the pregnancy and breastfeeding period. Available disease modifying treatment is discussed in the article with new data supporting the strategy of continuing natalizumab after conception, as it is related to a decreased risk of MS relapses during the pregnancy and postpartum period.
Mechanical Behavior Investigation of 4H-SiC Single Crystal at the Micro–Nano Scale
Authors: Chai Peng , Li Shujuan , Li Yan , Liang Lie , Yin Xincheng
Published: January 2020

In this paper, theoretical models of the critical indentation depth and critical force on brittle materials using cleavage strength and contact theory are proposed. A Berkovich indenter is adopted for nanoindentation tests on a 4H-SiC single crystal sample to evaluate its mechanical behaviors. The stages of brittle material deformation (elastic, plastic, and brittle) can be characterized by the load versus indentation depth curves through the nanoindentation test. The curve of the elastic deformation stage follows the Hertz contact theory, and plastic deformation occurs at an indentation depth of up to 10 nm. The mechanism of 4H-SiC single crystal cracking is discussed, and the critical indentation depth and critical force for the plastic–brittle transition are obtained through the occurrence of the pop-in point. This shows that the theoretical results have good coherence with the test results. Both the values of the elastic modulus and hardness decrease as the crack length increases. In order to obtain more accurate mechanical property values in the nanoindentation test for brittle materials such as SiC, GaN, and sapphire, an appropriate load that avoids surface cracks should be adopted.
Preparation and Characterization of Freely-Suspended Graphene Nanomechanical Membrane Devices with Quantum Dots for Point-of-Care Applications
Authors: Memisoglu Gorkem , Gulbahar Burhan , Fernandez Bello Ruben
Published: January 2020

We demonstrate freely suspended graphene-based nanomechanical membranes (NMMs) as acoustic sensors in the audible frequency range. Simple and low-cost procedures are used to fabricate NMMs with various thicknesses based on graphene layers grown by graphite exfoliation and solution processed graphene oxide. In addition, NMMs are grafted with quantum dots (QDs) for characterizing mass sensitive vibrational properties. Thickness, roughness, deformation, deflection and emissions of NMMs with attached QDs are experimented and analyzed by utilizing atomic force microscopy, Raman spectroscopy, laser induced deflection analyzer and spectrophotometers. Förster resonance energy transfer (FRET) is experimentally achieved between the QDs attached on NMMs and nearby glass surfaces for illustrating acousto-optic utilization in future experimental implementations combining vibrational properties of NMMs with optical emission properties of QDs. This property denoted as vibrating FRET (VFRET) is previously introduced in theoretical studies while important experimental steps are for the first time achieved in this study for future VFRET implementations. The proposed modeling and experimental methodology are promising for future novel applications such as NMM based biosensing, photonics and VFRET based point-of-care (PoC) devices.
Durability and Recoverability of Soft Lithographically Patterned Hydrogel Molds for the Formation of Phase Separation Membranes
Authors: Asad Asad , Rastgar Masoud , Nazaripoor Hadi , Sadrzadeh Mohtada , Sameoto Dan
Published: January 2020

Hydrogel-facilitated phase separation (HFPS) has recently been applied to make microstructured porous membranes by modified phase separation processes. In HFPS, a soft lithographically patterned hydrogel mold is used as a water content source that initiates the phase separation process in membrane fabrication. However, after each membrane casting, the hydrogel content changes due to the diffusion of organic solvent into the hydrogel from the original membrane solution. The absorption of solvent into the hydrogel mold limits the continuous use of the mold in repeated membrane casts. In this study, we investigated a simple treatment process for hydrogel mold recovery, consisting of warm and cold treatment steps to provide solvent extraction without changing the hydrogel mold integrity. The best recovery result was 96%, which was obtained by placing the hydrogel in a warm water bath (50 °C) for 10 min followed by immersing in a cold bath (23 °C) for 4 min and finally 4 min drying in air. This recovery was attributed to nearly complete solvent extraction without any deformation of the hydrogel structure. The reusability of hydrogel can assist in the development of a continuous membrane fabrication process using HFPS.
Detection of Specific Antibodies against Toscana Virus among Blood Donors in Northeastern Italy and Correlation with Sand Fly Abundance in 2014
Authors: Morini Silvia , Calzolari Mattia , Rossini Giada , Pascarelli Nadia , Porcellini Andrea , Randi Vanda , Re Maria Carla , Albieri Alessandro , Bonilauri Paolo , Bellini Romeo , Ayhan Nazli , Charrel Remi , Varani Stefania
Published: January 2020

Toscana virus (TOSV) is a Phlebovirus transmitted by phlebotomine sand flies and is an important etiological agent of summer meningitis in the Mediterranean basin. Since TOSV infection is often asymptomatic, we evaluated the seroprevalence in blood donors (BDs) in the Bologna and Ferrara provinces (Northeastern Italy)—the areas with the highest and lowest numbers of TOSV neuroinvasive cases in the region, respectively. A total of 1208 serum samples from BDs were collected in April–June 2014 and evaluated for the presence of specific TOSV-IgG by ELISA. The IgG-reactive samples were confirmed by indirect immunofluorescence assay (IIF) and by microneutralization test (MN). Serum samples were defined as positive for anti-TOSV IgG when reactive by ELISA and by at least one second-level test; TOSV seroprevalence was 6.8% in the Bologna province, while no circulation of TOSV was detected in the Ferrara province. Sand fly abundance in 2014 was also estimated by a geographic information system using a generalized linear model applied to a series of explanatory variables. TOSV seroprevalence rate was strongly associated with the sand fly abundance index in each municipality, pointing out the strong association between sand fly abundance and human exposure to TOSV.
Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant Staphylococcus aureus
Authors: Yang John Jackson , Huang Yung-Chi , Chuang Tsung-Hsien , Herr Deron Raymond , Hsieh Ming-Fa , Huang Chun-Jen , Huang Chun-Ming
Published: January 2020

Methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) has become an alarming threat to public health, and infected soft tissue. Antibiotics are commonly used to treat skin infection with MRSA, but the inappropriate use of antibiotics runs a considerable risk of generating resistant S. aureus. In this study, we created a cysteine-capped hydrogel able to absorb and release copper, an ion with the capability of suppressing the growth of USA300, a community-acquired MRSA. The results of analysis of Fourier transform infrared spectroscopy (FTIR) revealed the binding of copper to a cysteine-capped hydrogel. The topical application of a cysteine-capped hydrogel binding with copper on USA300-infected skin wounds in the dorsal skin of Institute of Cancer Research (ICR) mice significantly enhanced wound healing, hindered the growth of USA300, and reduced the production of pro-inflammatory macrophage inflammatory protein 2-alpha (MIP-2) cytokine. Our work demonstrates a newly designed hydrogel that conjugates a cysteine molecule for copper binding. The cysteine-capped hydrogel can potentially chelate various antimicrobial metals as a novel wound dressing.
Trypanocidal Activity of Flavanone Derivatives
Authors: Maciel Diogo Gabriela , Andrade Josimara Souza , Sales Junior Policarpo Ademar , Maria Fonseca Murta Silvane , Dos Santos Viviane Martins Rebello , Taylor Jason Guy
Published: January 2020

Chagas disease, also known as American trypanosomiasis, is classified as a neglected disease by the World Health Organization. For clinical treatment, only two drugs have been on the market, Benznidazole and Nifurtimox, both of which are recommended for use in the acute phase but present low cure rates in the chronic phase. Furthermore, strong side effects may result in discontinuation of this treatment. Faced with this situation, we report the synthesis and trypanocidal activity of 3-benzoyl-flavanones. Novel 3-benzoyl-flavanone derivatives were prepared in satisfactory yields in the 3-step synthetic procedure. According to recommended guidelines, the whole cell-based screening methodology was utilized that allowed for the simultaneous use of both parasite forms responsible for human infection. The majority of the tested compounds displayed promising anti-Trypanosoma cruzi activity and the most potent flavanone bearing a nitrofuran moiety was more potent than the reference drug, Benznidazole.
Chiral Thioureas—Preparation and Significance in Asymmetric Synthesis and Medicinal Chemistry
Authors: Steppeler Franz , Iwan Dominika , Wojaczyńska Elżbieta , Wojaczyński Jacek
Published: January 2020

For almost 20 years, thioureas have been experiencing a renaissance of interest with the emerged development of asymmetric organocatalysts. Due to their relatively high acidity and strong hydrogen bond donor capability, they differ significantly from ureas and offer, appropriately modified, great potential as organocatalysts, chelators, drug candidates, etc. The review focuses on the family of chiral thioureas, presenting an overview of the current state of knowledge on their synthesis and selected applications in stereoselective synthesis and drug development.
Photochemistry of the Cloud Aqueous Phase: A Review
Authors: Bianco Angelica , Passananti Monica , Brigante Marcello , Mailhot Gilles
Published: January 2020

This review paper describes briefly the cloud aqueous phase composition and deeply its reactivity in the dark and mainly under solar radiation. The role of the main oxidants (hydrogen peroxide, nitrate radical, and hydroxyl radical) is presented with a focus on the hydroxyl radical, which drives the oxidation capacity during the day. Its sources in the aqueous phase, mainly through photochemical mechanisms with H2O2, iron complexes, or nitrate/nitrite ions, are presented in detail. The formation rate of hydroxyl radical and its steady state concentration evaluated by different authors are listed and compared. Finally, a paragraph is also dedicated to the sinks and the reactivity of the HO• radical with the main compounds found in the cloud aqueous phase. This review presents an assessment of the reactivity in the cloud aqueous phase and shows the significant potential impact that this medium can have on the chemistry of the atmosphere and more generally on the climate.
Thymoquinone Enhances Paclitaxel Anti-Breast Cancer Activity via Inhibiting Tumor-Associated Stem Cells Despite Apparent Mathematical Antagonism
Authors: Bashmail Hanan A. , Alamoudi Aliaa A. , Noorwali Abdulwahab , Hegazy Gehan A. , Ajabnoor Ghada M. , Al-Abd Ahmed M.
Published: January 2020

Thymoquinone (TQ) has shown substantial evidence for its anticancer effects. Using human breast cancer cells, we evaluated the chemomodulatory effect of TQ on paclitaxel (PTX). TQ showed weak cytotoxic properties against MCF-7 and T47D breast cancer cells with IC50 values of 64.93 ± 14 µM and 165 ± 2 µM, respectively. Combining TQ with PTX showed apparent antagonism, increasing the IC50 values of PTX from 0.2 ± 0.07 µM to 0.7 ± 0.01 µM and from 0.1 ± 0.01 µM to 0.15 ± 0.02 µM in MCF-7 and T47D cells, respectively. Combination index analysis showed antagonism in both cell lines with CI values of 4.6 and 1.6, respectively. However, resistance fractions to PTX within MCF-7 and T47D cells (42.3 ± 1.4% and 41.9 ± 1.1%, respectively) were completely depleted by combination with TQ. TQ minimally affected the cell cycle, with moderate accumulation of cells in the S-phase. However, a significant increase in Pre-G phase cells was observed due to PTX alone and PTX combination with TQ. To dissect this increase in the Pre-G phase, apoptosis, necrosis, and autophagy were assessed by flowcytometry. TQ significantly increased the percent of apoptotic/necrotic cell death in T47D cells after combination with paclitaxel. On the other hand, TQ significantly induced autophagy in MCF-7 cells. Furthermore, TQ was found to significantly decrease breast cancer-associated stem cell clone (CD44+/CD24-cell) in both MCF-7 and T47D cells. This was mirrored by the downregulation of TWIST-1 gene and overexpression of SNAIL-1 and SNAIL-2 genes. TQ therefore possesses potential chemomodulatory effects to PTX when studied in breast cancer cells via enhancing PTX induced cell death including autophagy. In addition, TQ depletes breast cancer-associated stem cells and sensitizes breast cancer cells to PTX killing effects.
Acknowledgement to Reviewers of Molecules in 2019
Authors: Molecules Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not [...]
Diet-Induced Rodent Models of Diabetic Peripheral Neuropathy, Retinopathy and Nephropathy
Authors: Preguiça Inês , Alves André , Nunes Sara , Gomes Pedro , Fernandes Rosa , Viana Sofia D. , Reis Flávio
Published: January 2020

Unhealthy dietary habits are major modifiable risk factors for the development of type 2 diabetes mellitus, a metabolic disease with increasing prevalence and serious consequences. Microvascular complications of diabetes, namely diabetic peripheral neuropathy (DPN), retinopathy (DR), and nephropathy (DN), are associated with high morbidity rates and a heavy social and economic burden. Currently, available therapeutic options to counter the evolution of diabetic microvascular complications are clearly insufficient, which strongly recommends further research. Animal models are essential tools to dissect the molecular mechanisms underlying disease progression, to unravel new therapeutic targets, as well as to evaluate the efficacy of new drugs and/or novel therapeutic approaches. However, choosing the best animal model is challenging due to the large number of factors that need to be considered. This is particularly relevant for models induced by dietary modifications, which vary markedly in terms of macronutrient composition. In this article, we revisit the rodent models of diet-induced DPN, DR, and DN, critically comparing the main features of these microvascular complications in humans and the criteria for their diagnosis with the parameters that have been used in preclinical research using rodent models, considering the possible need for factors which can accelerate or aggravate these conditions.
Small-Scale Livestock Production in Nepal Is Directly Associated with Children’s Increased Intakes of Eggs and Dairy, But Not Meat
Authors: Broaddus-Shea Elena T. , Manohar Swetha , Thorne-Lyman Andrew L. , Bhandari Shiva , Nonyane Bareng A. S. , Winch Peter J. , West Keith P.
Published: January 2020

Animal source foods (ASF) provide nutrients essential to child growth and development yet remain infrequently consumed in rural Nepal. Agriculture and nutrition programs aim to increase ASF intake among children through small-scale animal husbandry projects. The relationship between livestock ownership and children’s consumption of ASF, however, is not well established. This study examined associations between livestock ownership and the frequency with which Nepali children consume eggs, dairy, and meat. We analyzed longitudinal 7-day food frequency data from sentinel surveillance sites of the Policy and Science of Health, Agriculture and Nutrition (PoSHAN) study. Data consisted of surveys from 485 Nepali farming households conducted twice per year for two years (a total of 1449 surveys). We used negative binomial regression analysis to examine the association between the number of cattle, poultry, and meat animals (small livestock) owned and children’s weekly dairy, egg, and meat intakes, respectively, adjusting for household expenditure on each food type, mother’s education level, caste/ethnicity, agroecological region, season, and child age and sex. We calculated predicted marginal values based on model estimates. Children consumed dairy 1.4 (95% CI 1.1–2.0), 2.3 (1.7–3.0) and 3.0 (2.1–4.2) more times per week in households owning 1, 2–4 and >4 cattle, respectively, compared to children in households without cattle. Children consumed eggs 2.8 (2.1–3.7) more times per week in households owning 1 or 2 chickens compared to children in households without chickens. Child intake of meat was higher only in households owning more than seven meat animals. Children’s intakes of dairy, eggs, and meat rose with household expenditure on these foods. Small-scale animal production may be an effective strategy for increasing children’s consumption of eggs and dairy, but not meat. Increasing household ability to access ASF via purchasing appears to be an important approach for raising children’s intakes of all three food types.
Effects of Lactobacillus plantarum Q180 on Postprandial Lipid Levels and Intestinal Environment: A Double-Blind, Randomized, Placebo-Controlled, Parallel Trial
Authors: Park Ye Eun , Kim Min Seo , Shim Kyung Won , Kim You-Il , Chu Jaeryang , Kim Byoung-Kook , Choi In Suk , Kim Ji Yeon
Published: January 2020

Probiotics can improve the intestinal environment by enhancing beneficial bacteria to potentially regulate lipid levels; however, the underlying mechanisms remain unclear. The aim of this study was to investigate the effect of Lactobacillus plantarum Q180 (LPQ180) on postprandial lipid metabolism and the intestinal microbiome environment from a clinical perspective. A double-blind, randomized, placebo-controlled study was conducted including 70 participants of both sexes, 20 years of age and older, with healthy blood triacylglyceride (TG) levels below 200 mg/dL. Treatment with LPQ180 for 12 weeks significantly decreased LDL-cholesterol (p = 0.042) and apolipoprotein (Apo)B-100 (p = 0.003) levels, and decreased postprandial maximum concentrations (Cmax) and areas under the curve (AUC) of TG, chylomicron TG, ApoB-48, and ApoB-100. LPQ180 treatment significantly decreased total indole and phenol levels (p = 0.019). In addition, there was a negative correlation between baseline microbiota abundance and lipid marker change, which was negatively correlated with metabolites. This study suggests that LPQ180 might be developed as a functional ingredient to help maintain healthy postprandial lipid levels through modulating gut environment.
Saliva Secretion and Swallowing—The Impact of Different Types of Food and Drink on Subsequent Intake
Authors: Bozorgi Catherina , Holleufer Celina , Wendin Karin
Published: January 2020

The oral processing of food is important for eating and digestion in order to gain energy and nutrients. Due to disease, injury, or aging, individuals may experience difficulties in this process. These difficulties often lead to dysphagia, which is associated with malnutrition. Thus, it is of importance to find solutions and strategies to enable food intake. It is well known that sour and/or carbonated foods and drinks increase saliva secretion and trigger the swallowing reflex. However, knowledge regarding how subsequent food intake is impacted is lacking. The aim of this study was to clarify whether sour and/or carbonated foods and drinks had subsequent impacts on swallowing function. Twelve healthy participants evaluated eleven foods and drinks in terms of their ability to increase saliva production and ease the swallowing of subsequent food. Results showed that sourness and carbonation had positive impacts on saliva secretion and swallowing. No correlation was found between the pH/sourness of the foods and the ease of swallowing them. It was concluded that the ingestion of cherry tomatoes, natural yoghurt, and, in particular, citrus juice made swallowing of a neutral cracker easier. These results may be used to increase food intake among dysphagia patients.