bluuebunny/crossref_metadata_2025 huggingface dataset:
Accuracy and longitudinal reproducibility of quantitative femorotibial cartilage measures derived from automated U-Net-based segmentation of two different MRI contrasts: data from the osteoarthritis initiative healthy reference cohort
Authors: Wirth Wolfgang , Eckstein Felix , Kemnitz Jana , Baumgartner Christian Frederik , Konukoglu Ender , Fuerst David , Chaudhari Akshay Sanjay
Published: October 2020

Objective

To evaluate the agreement, accuracy, and longitudinal reproducibility of quantitative cartilage morphometry from 2D U-Net-based automated segmentations for 3T coronal fast low angle shot (corFLASH) and sagittal double echo at steady-state (sagDESS) MRI.

Methods

2D U-Nets were trained using manual, quality-controlled femorotibial cartilage segmentations available for 92 Osteoarthritis Initiative healthy reference cohort participants from both corFLASH and sagDESS (n = 50/21/21 training/validation/test-set). Cartilage morphometry was computed from automated and manual segmentations for knees from the test-set. Agreement and accuracy were evaluated from baseline visits (dice similarity coefficient: DSC, correlation analysis, systematic offset). The longitudinal reproducibility was assessed from year-1 and -2 follow-up visits (root-mean-squared coefficient of variation, RMSCV%).

Results

Automated segmentations showed high agreement (DSC 0.89–0.92) and high correlations (r ≥ 0.92) with manual ground truth for both corFLASH and sagDESS and only small systematic offsets (≤ 10.1%). The automated measurements showed a similar test–retest reproducibility over 1 year (RMSCV% 1.0–4.5%) as manual measurements (RMSCV% 0.5–2.5%).

Discussion

The 2D U-Net-based automated segmentation method yielded high agreement compared with manual segmentation and also demonstrated high accuracy and longitudinal test–retest reproducibility for morphometric analysis of articular cartilage derived from it, using both corFLASH and sagDESS.
Flow of colloidal suspension and irreversibility analysis with aggregation kinematics of nanoparticles in a microchannel
Authors: Sindhu S. , Gireesha B. J.
Published: October 2020

The current exploration focuses on the ethylene glycol (EG) based nanoliquid flow in a microchannel. The effectiveness of the internal heat source and linear radiation is reflected in the present investigation. The estimation of suitable thermal conductivity model has affirmative impact on the convective heat transfer phenomenon. The examination is conceded with the nanoparticle aggregation demonstrated by the Maxwell-Bruggeman and Krieger-Dougherty models which tackle the formation of nanolayer. These models effectively describe the thermal conductivity and viscosity correspondingly. The dimensionless mathematical expressions are solved numerically by the Runge Kutta Fehlberg approach. A higher thermal field is attained for the Bruggeman model due to the formation of thermal bridge. A second law analysis is carried out to predict the sources of irreversibility associated with the thermal system. It is remarked that lesser entropy generation is obtained for the aggregation model. The entropy generation rate declines with the slip flow and the thermal heat flux. A notable enhancement in the Bejan number is attained by increasing the Biot number. It is established that the nanoparticle aggragation model exhibits a higher Bejan number in comparision with the usual flow model.
Systematic review of sourcing and 3D printing: make-or-buy decisions in industrial buyer–supplier relationships
Authors: Meyer Matthias M. , Glas Andreas H. , Eßig Michael
Published: October 2020

Additive manufacturing (AM) is regarded as a technology that has transformative and disruptive potential in nearly all industries. However, AM is not only about new production equipment and processes. Given the decreasing degree of vertical integration in many companies, suppliers add significant value to the finished product. AM might lead to the redesign of production networks, including a scenario in which the buyer uses AM to produce parts with data provided by suppliers. Overall, AM could have a major impact on the ways in which buyers and suppliers collaborate in the future. Nevertheless, research on AM in the field of industrial procurement remains scarce. This is surprising, given that AM is not only changing traditional procurement categories and creating new ones (comprising printers, powder raw materials, data and the associated engineering services) but AM’s widely discussed potential for decentralisation might also restructure the logistical aspects (transport, stocks) of supply chains. In addition, AM may resurrect the old procurement question of ‘make or buy’. Current research focuses on the logistical aspects of AM and concerns such issues of decentralisation (such as the diminishing need for transportation and the design of transport networks). In contrast, this research addresses the question of whether AM demands new answers to strategic sourcing questions. For this purpose, academic journal literature concerning procurement and AM search strings is reviewed. Selected articles are analysed using a fine-grained analytical framework of procurement strategies. The findings show that existing research lacks theoretical approaches and a systematic view of the topic. Specifically, the analysis reveals a number of distinct knowledge gaps, which present several potential directions for future research.
Adipose Morphology: a Critical Factor in Regulation of Human Metabolic Diseases and Adipose Tissue Dysfunction
Authors: Liu Fangcen , He Jielei , Wang Hongdong , Zhu Dalong , Bi Yan
Published: October 2020

Emerging evidence highlights that dysfunction of adipose tissue contributes to impaired insulin sensitivity and systemic metabolic deterioration in obese state. Of note, adipocyte hypertrophy serves as a critical event which associates closely with adipose dysfunction. An increase in cell size exacerbates hypoxia and inflammation as well as excessive collagen deposition, finally leading to metabolic dysregulation. Specific mechanisms of adipocyte hypertrophy include dysregulated differentiation and maturation of preadipocytes, enlargement of lipid droplets, and abnormal adipocyte osmolarity sensors. Also, weight loss therapies exert profound influence on adipocyte size. Here, we summarize the critical role of adipocyte hypertrophy in the development of metabolic disturbances. Future studies are required to establish a standard criterion of size measurement to better clarify the impact of adipocyte hypertrophy on changes in metabolic homeostasis.
The Complex Management of Atrial Fibrillation and Cancer in the COVID-19 Era: Drug Interactions, Thromboembolic Risk, and Proarrhythmia
Authors: Gatti Milo , Raschi Emanuel , Poluzzi Elisabetta , Martignani Cristian , Salvagni Stefania , Ardizzoni Andrea , Diemberger Igor
Published: October 2020

Purpose of Review

Cardiotoxicity by anticancer agents has emerged as a multifaceted issue and is expected to affect both mortality and morbidity. This review summarizes clinical challenges in the management of oncological patients requiring anticoagulants for atrial fibrillation (AF) also considering the current outbreak of the COVID-19 (coronavirus disease 2019) pandemic, since this infection can add challenges to the management of both conditions. Specifically, the aims are manyfold: (1) describe the evolving use of direct oral anticoagulants (DOACs) in AF patients with cancer; (2) critically appraise the risk of clinically important drug-drug interactions (DDIs) between DOACs and oral targeted anticancer agents; (3) address expected DDIs between DOACs and candidate anti-COVID drugs, with implications on management of the underlying thrombotic risk; and (4) characterize the proarrhythmic liability in cardio-oncology in the setting of COVID-19, focusing on QT prolongation.

Recent Findings

AF in cardio-oncology poses diagnostic and management challenges, also due to the number of anticancer drugs recently associated with AF onset/worsening. Oral targeted drugs can potentially interact with DOACs, with increased bleeding risk mainly due to pharmacokinetic DDIs. Moreover, the vast majority of oral anticancer agents cause QT prolongation with direct and indirect mechanisms, potentially resulting in the occurrence of torsade de pointes, especially in susceptible patients with COVID-19 receiving additional drugs with QT liability.

Oncologists and cardiologists must be aware of the increased bleeding risk and arrhythmic susceptibility of patients with AF and cancer due to DDIs. High-risk individuals with COVID-19 should be prioritized to target preventive strategies, including optimal antithrombotic management, medication review, and stringent monitoring.
The effects of grain size and different multi-stage shearing techniques on shear strength along rock discontinuities
Authors: Vattai Alina , Rozgonyi-Boissinot Nikoletta
Published: October 2020

The effects of grain size and different multi-stage shearing techniques on shear strength along discontinuities were analyzed in this study. Laboratory direct shear tests were carried out on plaster mortar with maximum grain sizes of 0.5 mm and 1.0 mm. All specimen surfaces were essentially similar, copied from the same natural, Hungarian coarse-grained sandstone joint with a low joint roughness coefficient (JRC = 8). Tests within two different normal stress ranges (σn = 0.25–0.5 and 0.5–1.5 MPa) were performed simultaneously. Specimens tested using the technique involving modified shearing with repositioning were sheared three times while being subjected to the same degree of normal stress (shearing sequence n = 1, 2, 3) and those with multi-stage technique without repositioning were subjected to shearing once at three different degrees of normal stress. The changing values of the peak friction angle calculated from the resulting peak shear strength-normal stress data pairs (τp − σn) were examined. Failure curves were estimated using linear regression, according to the Mohr–Coulomb failure criterion. The differences between the various peak friction angles obtained from experiments in which different multi-stage shearing techniques were used tend to increase in significance with the increasing number of shearing sequences. Peak friction angle values vary according to grain size of the material, though further investigations using more grain sizes are required to establish the extent of the effect on shear strength along discontinuities.
A Micro Structure POF Relative Humidity Sensor Modified With Agarose Based on Surface Plasmon Resonance and Evanescent Wave Loss
Authors: Hu Yanjun , Ghaffar Abdul , Hou Yulong , Liu Wenyi , Li Fei , Wang Jun
Published: October 2020

A novel high sensitivity relative humidity (RH) sensor was proposed by using micro structure plastic optical fiber (POF) based on the surface plasmon resonance (SPR) effect and the evanescent wave (EW) loss. The micro structure was fabricated on the POF and coated with a gold layer and agarose, adopting the sputtering and dip-coating technique. These construction effects on the attenuation of power caused by the SPR effect and the EW loss were used to perform RH detections. The agarose’s different refractive indexes (RIs) caused fluctuations in the transmission power when the humidity increased. The demonstrated experimental results showed that the proposed sensor achieved a linear response from 20% RH to 80% RH with a high sensitivity of 0.595µW/%. The proposed sensor had the advantages of fast response and recovery. Furthermore, the temperature dependence and the repeatability test of the sensor were also performed.
An approach to lifting self-isolation for health care workers with prolonged shedding of SARS-CoV-2 RNA
Authors: Laferl H. , Kelani H. , Seitz T. , Holzer B. , Zimpernik I. , Steinrigl A. , Schmoll F. , Wenisch C. , Allerberger F.
Published: October 2020

Purpose

According to the European Public Health Authority guidance for ending isolation in the context of COVID-19, a convalescent healthcare worker (HCW) can end their isolation at home and resume work upon clinical improvement and two negative RT-PCR tests from respiratory specimens obtained at 24-h intervals at least 8 days after the onset of symptoms. However, convalescent HCWs may shed SARS-CoV-2 viral RNA for prolonged periods.

Methods

40 healthy HCWs off work because of ongoing positive RT-PCR results in combined nasopharyngeal (NP) and oropharyngeal (OP) swabs following SARS-CoV-2 infection were invited to participate in this study. These HCWs had been in self-isolation because of a PCR-confirmed SARS-CoV-2 infection. NP and OP swabs as well as a blood sample were collected from each participant. RT-PCR and virus isolation was performed with each swab sample and serum neutralization test as well as two different ELISA tests were performed on all serum samples.

Results

No viable virions could be detected in any of 29 nasopharyngeal and 29 oropharyngeal swabs taken from 15 long-time carriers. We found SARSCoV- 2 RNA in 14/29 nasopharyngeal and 10/29 oropharyngeal swabs obtained from screening 15 HCWs with previous COVID-19 up to 55 days after symptom onset. Six (40%) of the 15 initially positive HCWs converted to negative and later reverted to positive again according to their medical records. All but one HCW, a healthy volunteer banned from work, showed the presence of neutralizing antibodies in concomitantly taken blood samples. Late threshold cycle (Ct) values in RT-PCR [mean 37.4; median 37.3; range 30.8–41.7] and the lack of virus growth in cell culture indicate that despite the positive PCR results no infectivity remained.

Conclusion

We recommend lifting isolation if the RT-PCR Ct-value of a naso- or oropharyngeal swab sample is over 30. Positive results obtained from genes targeted with Ct-values > 30 correspond to non-viable/noninfectious particles that are still detected by RT-PCR. In case of Ct-values lower than 30, a blood sample from the patient should be tested for the presence of neutralizing antibodies. If positive, non-infectiousness can also be assumed.
The Polish version of the Düsseldorf Orthorexia Scale (PL-DOS) and its comparison with the English version of the DOS (E-DOS)
Authors: Brytek-Matera Anna
Published: October 2020

Purpose

Although orthorexia nervosa, the fixation on health-conscious eating behaviour, was first described in the 90 s, there is no clear understanding whether existing ON measures are appropriate for its assessment. The objectives of the present study were to: (1) examine the psychometric properties of the Polish version of the DOS (PL-DOS) and to compare the PL-DOS with the English version of the DOS (E-DOS) as well as (2) evaluate the prevalence of ON among Polish university students and compare the prevalence rates of ON among Polish and U.S. students.

Methods

Four-hundred and twelve students (77.2% female) with a mean age of 24.62 years (SD = 6.86) participated in the present study. All participants completed the Polish version of the Düsseldorf Orthorexia Scale (PL-DOS), the Eating Habits Questionnaire (EHQ) and the Eating Disorder Inventory (EDI).

Results

Reliability analysis for the PL-DOS showed strong internal consistency with a Cronbach’s alpha coefficient of 0.840 and a coefficient omega of 0.840, 95% CI [0.808, 0.866]. Significant correlation coefficients were found between the PL-DOS and all subscales of the EHQ. Confirmatory factor analysis showed that the one-factor model had poor fit. Polish students had an ON prevalence rate of 6.6%, lower than that of U.S. students (8%).

Conclusions

Our findings validate the use of the PL-DOS as an appropriate ON measure for a Polish population.

Level of evidence

Level V, cross-sectional descriptive study.
First assessment of habitat suitability and connectivity for the golden jackal in north-eastern Italy
Authors: Torretta Elisa , Dondina Olivia , Delfoco Claudio , Riboldi Luca , Orioli Valerio , Lapini Luca , Meriggi Alberto
Published: October 2020

Compared with the rapid expansion across Europe, the golden jackal colonization of Italy is still limited and slow. No study focused on the habitat selection or landscape connectivity for this species was performed in Italy; thus, the potential distribution and dispersal patterns in the country remain unknown. Our objectives were to evaluate the suitability of the Friuli-Venezia Giulia region (north-eastern Italy) for the golden jackal, as well as to identify the ecological corridors connecting the areas currently occupied by the species. Corridors modelling allowed us both to hypothesize the dispersal dynamics occurring in the study region and to identify possible obstacles to future range expansion. We surveyed golden jackal presence in two study areas, covering an area of 500 km2, from March 2017 to February 2018. Using collected data, we modelled the species home-range scale habitat suitability based on an ensemble modelling approach. Subsequently, a habitat suitability prediction at a finer scale was used to estimate landscape resistance, starting from which, we modelled dispersal corridors among areas currently occupied by the species using a factorial least cost path and a cumulative resistant kernel approach. Our results indicated a moderate potential for large parts of the study region to support the occurrence of golden jackal family groups, whose presence seems to be mainly driven by the presence of wide areas covered by broadleaved forests and shrublands and by the absence of wide intensive agricultural areas. The predicted connectivity networks showed that three main permeable corridors are likely to connect golden jackal occurrence areas within the study region, while all the other corridors are characterized by a very low path density. Both the habitat selection and connectivity analyses showed a strong negative impact of the intensive cultivated plain on species stable presence and movement providing critical information for the conservation of the golden jackal in Italy.
On the Offensive
Authors: Stollznow Karen
Published: October 2020

I'm not a racist, but… You look good, for your age… She was asking for it… You're crazy… That's so gay… Have you ever wondered why certain language has the power to offend? It is often difficult to recognize the veiled racism, sexism, ageism (and other –isms) that hide in our everyday discourse. This book sheds light on the derogatory phrases, insults, slurs, stereotypes, tropes and more that make up linguistic discrimination. Each chapter addresses a different area of prejudice: race and ethnicity; gender identity; sexuality; religion; health and disability; physical appearance; and age. Drawing on hot button topics and real-life case studies, and delving into the history of offensive terms, a vivid picture of modern discrimination in language emerges. By identifying offensive language, both overt and hidden, past and present, we uncover vast amounts about our own attitudes, beliefs and values and reveal exactly how and why words can offend.
Essential Neuropharmacology
Authors: Silberstein Stephen D. , Marmura Michael J. , Yuan Hsiangkuo
Published: October 2020

Essential Neuropharmacology: The Prescriber's Guide reviews the full range of medications used by neurologists in clinical practice. Expert knowledge is given about the best use of medications in patient care. Each drug listing contains the full range of indications, their advantages and disadvantages, and tips for dosing and avoiding adverse effects. Evidence is taken from recent clinical trials, which helps the reader relate the drug descriptions to everyday clinical practice. Where appropriate, off-label uses are also described. The detailed descriptions of each medication enable the user to make quick and informed decisions with the confidence they need to serve the clinical needs of their patients effectively. The new edition is fully updated throughout. Major new additions include neuro-oncology drugs, new multiple sclerosis agents, sleep medications and new formulations. This book is an essential, user-friendly reference suitable for neurologists at all stages of their careers.
The Philosophy of Emil Fackenheim
Authors: Green Kenneth Hart
Published: October 2020

Fackenheim was one of the philosophically serious, knowledgeable, and provocative contemporary Jewish thinkers.  His original focus as a philosophical theologian was mainly on revelation, but in his later work he concerned himself primarily with the wide-ranging  implications of the  Holocaust. In this book, Kenneth Green examines Fackenheim's intellectual trajectory and traces how and why he focused so intently on the Holocaust.  He explores the deeper thought that Fackenheim developed about the Holocaust, which he construed as a cataclysmic event that ruptured history and one that also brought about a change in the very structure of being. As Green demonstrates, the Holocaust, according to Fackenheim's interpretation, changes how we view all things, from God to man to history. It also radically affects Judaism, Christianity, and philosophy, the major traditions that have shaped the Western world.
The Films of Konrad Wolf
Authors: Powell Larson
Published: October 2020

This is the first book in any language on the films of Konrad Wolf (1925–1982), East Germany's greatest filmmaker, and puts Wolf in a larger European filmic and historical context.
Have Europeans Grown Tired of Democracy? New Evidence from Eighteen Consolidated Democracies, 1981–2018
Authors: Wuttke Alexander , Gavras Konstantin , Schoen Harald
Published: October 2020

Democracies without democrats are not sustainable. Yet, recent studies have argued that Western citizens are turning their backs on the system of self-governance, thereby eroding the societal foundations of consolidated democracies. This study contributes to discussions about citizen support of democracy by (1) analyzing new cross-national survey data in 18 European countries that facilitate assessments of the temporal and geographical generalizability of previous findings, (2) disentangling age, cohort and period effects, thereby aligning the analytical methods with the theoretical arguments and (3) transparently reporting all evidence derived from pre-registered analyses to avoid cherry-picked findings. The findings show that citizens of consolidated democracies continue to endorse self-governance. Yet in some (but not all) countries, there is evidence of a growing number of ‘democrats in name only’, particularly among the young generation. These findings suggest a second phase in research on democratic fatigue that broadens the analytical scope for the multi-faceted nature of democratic support.
Over-under cartilage tympanoplasty: technique, results and a call for improved reporting
Authors: Erbele I D , Fink M R , Mankekar G , Son L S , Mehta R , Arriaga M A
Published: October 2020

Objective

This study aimed to describe the microscopic over-under cartilage tympanoplasty technique, provide hearing results and detail clinically significant complications.

Method

This was a retrospective case series chart review study of over-under cartilage tympanoplasty procedures performed by the senior author between January 2015 and January 2019 at three tertiary care centres. Cases were excluded for previous or intra-operative cholesteatoma, if a mastoidectomy was performed during the procedure or if ossiculoplasty was performed. Hearing results and complications were obtained.

Results

Sixty-eight tympanoplasty procedures met the inclusion criteria. The median age was 13 years (range, 3–71 years). The mean improvement in pure tone average was 6 dB (95 per cent confidence interval 4–9 dB;p <0.0001). The overall perforation closure rate was 97 per cent (n =66). Revision surgery was recommended for a total of 6 cases (9 per cent) including 2 post-operative perforations, 1 case of middle-ear cholesteatoma and 3 cases of external auditory canal scarring.

Conclusion

Over-under cartilage tympanoplasty is effective at improving clinically meaningful hearing with a low rate of post-operative complications.
Formulating other minds in social interaction: Accountability and courses of action
Authors: Zinken Jörg , Kaiser Julia
Published: October 2020

We examine moments in social interaction in which a person formulates what another thinks or believes. Such formulations of belief constitute a practice with specifiable contexts and consequences. Belief formulations treat aspects of the other person's prior conduct as accountable on the basis that it provided a new angle on a topic, or otherwise made a surprising contribution within an ongoing course of actions. The practice of belief formulations subjectivizes the content that the other articulated and thereby topicalizes it, mobilizing commitment to that position, an account, or further elaboration. We describe how the practice can be put to work in different activity contexts: sometimes it is designed to undermine the other's position as a subjective ‘mere belief’, at other times it serves to mobilize further topic talk. Throughout, belief formulations show themselves to be a method by which we get to know ourselves and each other as mental agents. (Accountability, beliefs, courses of action, formulations, inference, subjectivity, topicalization)*
Probing linguistic change in Arabic vernaculars: A sociohistorical perspective
Authors: Al-Wer Enam , Horesh Uri , Alammar Deema , Alaodini Hind , Al-Essa Aziza , Al-Hawamdeh Areej , Al-Qahtani Khairia , Hussain Abeer Ab
Published: October 2020

It is received wisdom in variationist sociolinguistics that linguistic and social factors go hand in hand in structuring variability in language and any consequent instances of language change. We address the complexity of such factors by exploring data from several Arabic dialects in the eastern Arab world. We demonstrate that language change does not always follow expected phonological trajectories, even in cases where older changes are reconstructed to have operated along so-called universal patterns. In our explanation of recent changes in these dialects, we emphasise the role of social motivations for language change and the interactions between these social constraints and purely linguistic ones. Our analysis of change is supported by historical accounts of variation and change in Arabic. We illustrate how general principles of sociolinguistic theory apply to the Arabic data and provide additional layers of sociolinguistic information that highlight the importance of diverse data for evaluating cross-linguistic generalisations. (Arabic, language change, historical sociolinguistics)
Phillips Curve: The Greek Case
Authors: Liargovas Panagiotis , Psychalis Marios
Published: October 2020

One of the most important problems facing Greece is the long-term and high-level unemployment rate. The Economic Adjustment Programmes (EAPs) focused on the supply side of the economy, aiming at the adjustment of prices and wages, draw on the classical economic model, as it is widely accepted that internal devaluation policies keep inflation low. This article attempts to examine whether the Keynesian theory and the Phillips Curve, which shows the relationship between unemployment and inflation, apply in the case of the Greek economy. We use descriptive statistics, ordinary least squares (OLS) and VAR Analysis to examine the relationship between the variables. According to the results, there is a negative correlation between unemployment and inflation in Greece, thus confirming the Phillips Curve hypothesis. Finally, results show that unemployment is less dependent on inflation compared with the past, and there are numerous other decisive factors affecting unemployment.
WHAT IS NATURALISM?
Authors: Antony Louise
Published: October 2020

Louise Antony explains a variety of naturalisms, and why she doesn't believe in God.
NATURALISMS
Authors: Bryant Amanda
Published: October 2020

The word ‘naturalism’ has a bewildering array of uses in philosophy. Roughly speaking, it connotes pro-scientific attitudes and approaches. This article introduces the subject of naturalism by sketching a history of pro-scientific attitudes and approaches in philosophy, from their origins in the early modern period through to the present day. It then distinguishes a number of distinct families of naturalism: metaphysical, logico-linguistic, epistemological, and methodological. The resulting taxonomy encompasses a plurality of loosely related views rather than a number of variations on a single clear and unified theme.
NATURALISM AND THEISM
Authors: Lennox John C.
Published: October 2020

This article makes a case for theism by comparing it to naturalism. It examines the conflict between the two world-views, the nature of ultimate reality, the limits of science, the status of the universe, the role of faith in shaping beliefs, and how we account for human rationality. It concludes that naturalism is fatally flawed and that theism provides a more coherent explanation for human rationality and consciousness, the intelligibility of the universe and the existence of immaterial information.
Patient experience of Guided self-help CBT intervention for VoicEs (GiVE) delivered within a pilot randomized controlled trial
Authors: Hazell Cassie M. , Kelly Orla , O’Brien Sorcha , Strauss Clara , Cavanagh Kate , Hayward Mark
Published: October 2020

Access to cognitive behaviour therapy for those with psychosis (CBTp) remains poor. The most frequently endorsed barrier to implementation is a lack of resources. To improve access to CBTp, we developed a brief form of CBTp that specifically targets voice-related distress. The results of our pilot trial of guided self-help CBT for voices (GiVE) suggest that the therapy is both acceptable and beneficial. The present study aims to explore the subjective patient experience of accessing GiVE in the context of a trial. We interviewed nine trial participants using the Change Interview and a mixed methods approach. Most participants reported at least one positive change that they attributed to GiVE. We extracted five themes: (1) changes that I have noticed; (2) I am not alone; (3) positive therapy experiences; (4) I want more therapy; and (5) helping myself. The themes indicate that participating in the GiVE trial was generally a positive experience. The main areas in which participants experienced changes were improved self-esteem, and the ability to cope with voices. Positive changes were facilitated by embracing and enacting ‘self-help’ and having support both in and out of the therapy sessions. The findings support the use of self-help materials with those distressed by hearing voices, but that support both within and outside the clinical setting can aid engagement and outcomes. Overall, the findings support the continued investigation of GiVE.

Key learning aims

(1)

To explore participants’ experience of accessing GiVE as part of a trial.

(2)

To identify what (if any) changes participants noticed over the course of the GiVE trial.

(3)

To identify what participants attribute these changes to.
Spatial Heterogeneity in Particle‐Associated, Light‐Independent Superoxide Production Within Productive Coastal Waters
Authors: Sutherland Kevin M. , Grabb Kalina C. , Karolewski Jennifer S. , Plummer Sydney , Farfan Gabriela A. , Wankel Scott D. , Diaz Julia M. , Lamborg Carl H. , Hansel Colleen M.
Published: October 2020

In the marine environment, the reactive oxygen species (ROS) superoxide is produced through a diverse array of light‐dependent and light‐independent reactions, the latter of which is thought to be primarily controlled by microorganisms. Marine superoxide production influences organic matter remineralization, metal redox cycling, and dissolved oxygen concentrations, yet the relative contributions of different sources to total superoxide production remain poorly constrained. Here we investigate the production, steady‐state concentration, and particle‐associated nature of light‐independent superoxide in productive waters off the northeast coast of North America. We find exceptionally high levels of light‐independent superoxide in the marine water column, with concentrations ranging from 10 pM to in excess of 2,000 pM. The highest superoxide concentrations were particle associated in surface seawater and in aphotic seawater collected meters off the seafloor. Filtration of seawater overlying the continental shelf lowered the light‐independent, steady‐state superoxide concentration by an average of 84%. We identify eukaryotic phytoplankton as the dominant particle‐associated source of superoxide to these coastal waters. We contrast these measurements with those collected at an off‐shelf station, where superoxide concentrations did not exceed 100 pM, and particles account for an average of 40% of the steady‐state superoxide concentration. This study demonstrates the primary role of particles in the production of superoxide in seawater overlying the continental shelf and highlights the importance of light‐independent, dissolved‐phase reactions in marine ROS production.
Geomagnetically Induced Currents and Harmonic Distortion: High Time Resolution Case Studies
Authors: Clilverd Mark A. , Rodger Craig J. , Brundell James B. , Dalzell Michael , Martin Ian , Mac Manus Daniel H. , Thomson Neil R.
Published: October 2020

High time resolution (1–5 s) magnetometer, geomagnetically induced current (GIC), and mains harmonic distortion data from the Halfway Bush substation in Dunedin, New Zealand, are analyzed. A recently developed technique using very low frequency (VLF) radio wave data provides high‐resolution measurements of mains harmonic distortion levels. Three case studies are investigated, each involving high rates of change of local geomagnetic field, but with different timescales of magnetospheric driver mechanisms, and different substation transformer configurations. Two cases of enhanced GIC during substorm events are analyzed, and one case of a storm sudden commencement. Time delays between magnetic field fluctuations and induced transformer currents are found to be ~100 s for substorm events, but only ~20 s for the storm sudden commencement containing higher‐frequency variations. Boxcar averaging of the magnetic field fluctuations using running windows of ±2 min leads to spectral power profiles similar to those of GIC profiles, with reduced power at frequencies >0.003 Hz (periods <5 min). Substantially lower mains harmonic distortion levels were observed after the removal of the single phase bank transformer, HWB T4, from the high‐voltage configuration at Halfway Bush. No systematic time delay was found between GIC variations and mains harmonic distortion levels. The power spectra of magnetic field fluctuations and GIC variations during the sudden storm commencement with no harmonic distortion showed low levels of low‐frequency power (<0.003 Hz, periods >5 min). This low‐frequency component of the magnetic field power spectrum appears necessary for mains harmonic distortion to occur.
Local and Reach‐Scale Hyporheic Flow Response From Boulder‐Induced Geomorphic Changes
Authors: Dudunake T. , Tonina D. , Reeder W. J. , Monsalve A.
Published: October 2020

Stream hydromorphology regulates in‐stream water flow and interstitial flow of water within streambed sediments, the latter known as hyporheic exchange. Whereas hyporheic flow has been studied in sand‐bedded streams with ripples and dunes and in gravel‐bedded streams with pool‐riffle morphology, little is known about its characteristics in plane bed morphology with subdued streambed undulations and sparse macroroughness elements such as boulders and cobbles. Here, we present a proof‐of‐concept investigation on the role of boulder‐induced morphological changes on hyporheic flows based on coupling large‐scale flume sediment transport experiments with computational fluid dynamics. Our results show that placement of boulders on plane beds increase the reach‐scale hyporheic median residence time, τ50, by 15% and downwelling flux, qd, by 18% from the plane bed. However, reach‐scale hyporheic exchange changes are stronger with τ50 decreasing by 20% and qd increasing by 79% once the streambed morphology reached equilibrium (with the imposed upstream sediment and flow inputs on boulders). These results suggest that hyporheic flow is sensitive to the geomorphic response from bed topography and sediment transport in gravel‐bedded streams, a process that has been overlooked in previous work.
CD44/HA signaling mediates acquired resistance to a PI3Kα inhibitor
Authors: Yang Cuixia , Sheng Yumeng , Shi Xiaoxing , Liu Yiwen , He Yiqing , Du Yan , Zhang Guoliang , Gao Feng
Published: October 2020

Most luminal breast carcinomas (BrCas) bearing PIK3CA mutations initially respond to phosphoinositide-3-kinase (PI3K)-α inhibitors, but many eventually become resistant. The underlying mechanisms of this resistance remain obscure. In this work, we showed that a CD44high state due to aberrant isoform splicing was acquired from adaptive resistance to a PI3Kα inhibitor (BLY719) in luminal BrCas. Notably, the expression of CD44 was positively correlated with estrogen receptor (ER) activity in PIK3CA-mutant breast cancers, and ER-dependent transcription upon PI3Kα pathway inhibition was in turn mediated by CD44. Furthermore, the interaction of CD44 with the ligand hyaluronan (HA) initiated the Src-ERK signaling cascade, which subsequently maintained AKT and mTOR activity in the presence of a PI3Kα inhibitor. Activation of this pathway was prevented by disruption of the CD44/HA interaction, which in turn restored sensitivity to BLY719. Our results revealed that an ER-CD44-HA signaling circuit that mediates robust compensatory activation of the Src-ERK signaling cascade may contribute to the development of acquired resistance to PI3Kα inhibitors. This study provides new insight into the mechanism of adaptive resistance to PI3Kα inhibition therapy.
Simulations on the dual effects of flavonoids as suppressors of Aβ42 fibrillogenesis and destabilizers of mature fibrils
Authors: Gargari Sahar Andarzi , Barzegar Abolfazl
Published: October 2020

Structural studies of the aggregation inhibition of the amyloid-β peptide (Aβ) by different natural compounds are of the utmost importance due to their great potential as neuroprotective and therapeutic agents for Alzheimer’s disease. We provided the simulation of molecular dynamics for two different states of Aβ42, including “monomeric aggregation-prone state (APS)” and “U-shaped pentamers of amyloidogenic protofilament intermediates” in the absence and presence of polyphenolic flavonoids (Flvs, myricetin and morin) in order to verify the possible mechanism of Flvs fibrillogenesis suppression. Data showed that Flvs directly bind into Aβ42 species in both states of “monomeric APS β-sheets” and “pentameric amyloidogenic intermediates”. Binding of Flvs with amyloidogenic protofilament intermediates caused the attenuation of some inter-chains H-bonds, salt bridges, van der Waals and interpeptide interaction energies without interfering with their secondary β-sheets. Therefore, Flvs redirect oligomeric amyloidogenic intermediates into unstructured aggregates by significant disruption of the "steric zipper" motif of fibrils—pairs of self-complementary β-sheets—without changing the amount of β-sheets. It is while Flvs completely destruct the disadvantageous secondary β-sheets of monomeric APS conformers by converting them into coil/helix structures. It means that Flvs suppress the fibrillogenesis process of the monomeric APS structures by converting their β-sheets into proper soluble coil/helices structures. The different actions of Flvs in contact with two different states of Aβ conformers are related to high interaction tendency of Flvs with additional H-bonds for monomeric APS β-sheet, rather than oligomeric protofilaments. Linear interaction energy (LIE) analysis confirmed the strong binding of monomeric Aβ-Flvs with more negative ∆Gbinding, rather than oligomeric Aβ-Flvs system. Therefore, atomic scale computational evaluation of Flvs actions demonstrated different dual functions of Flvs, concluded from the application of two different monomeric and pentameric Aβ42 systems. The distinct dual functions of Flvs are proposed as suppressing the aggregation by converting β-sheets of monomeric APS to proper soluble structures and disrupting the "steric zipper" fibril motifs of oligomeric intermediate by converting on-pathway into off-pathway. Taken together, our data propose that Flvs exert dual and more effective functions against monomeric APS (fibrillogenesis suppression) and remodel the Aβ aggregation pathway (fibril destabilization).
Fabrication and characteristics of highly $$\langle {110} \rangle $$-oriented nanotwinned Au films
Authors: Chiu Wei-Lan , Liu Chien-Min , Lin Han-wen , Wu John A. , Chou Y.-C. , Tu K. N. , Chen Chih
Published: October 2020

Fine grained and nanotwinned Au has many excellent properties and is widely used in electronic devices. We have fabricated $$\langle {110} \rangle $$

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preferred-oriented Au thin films by DC plating at 5 mA/cm2. Microstructure analysis of the films show a unique fine grain structure with a twin formation. Hardness tests performed on electroplated $$\langle {110} \rangle $$

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Au films show a hardness 47% greater than random and untwinned Au. We then achieved direct bonding between two Au $$\langle {110} \rangle $$

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surfaces operating at 200 °C for an hour in a vacuum oven. The highly-oriented $$\langle {110} \rangle $$

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nanotwinned Au films could be an ideal material in many gold products.
Heat transfer flow of Maxwell hybrid nanofluids due to pressure gradient into rectangular region
Authors: Chu Yu-Ming , Ali Rizwan , Asjad Muhammad Imran , Ahmadian Ali , Senu Norazak
Published: October 2020

In this work, influence of hybrid nanofluids (Cu and$$\mathrm{Al}_{2}\mathrm{O}_{3}$$Al2O3) on MHD Maxwell fluid due to pressure gradient are discussed. By introducing dimensionless variables the governing equations with all levied initial and boundary conditions are converted into dimensionless form. Fractional model for Maxwell fluid is established by Caputo time fractional differential operator. The dimensionless expression for concentration, temperature and velocity are found using Laplace transform. As a result, it is found that fluid properties show dual behavior for small and large time and by increasing volumetric fraction temperature increases and velocity decreases respectively. Further, we compared the Maxwell, Casson and Newtonian fluids and found that Newtonian fluid has greater velocity due to less viscosity. Draw the graphs of temperature and velocity by Mathcad software and discuss the behavior of flow parameters and the effect of fractional parameters.
Rab6 is required for rapid, cisternal-specific, intra-Golgi cargo transport
Authors: Dickson Lindsey James , Liu Shijie , Storrie Brian
Published: October 2020

Rab6, the most abundant Golgi associated small GTPase, consists of 2 equally common isoforms, Rab6A and Rab6A′, that differ in 3 amino acids and localize to trans Golgi cisternae. The two isoforms are largely redundant in function and hence are often referred to generically as Rab6. Rab6 loss-of-function inhibits retrograde Golgi trafficking, induces an increase in Golgi cisternal number in HeLa cells and delays the cell surface appearance of the anterograde cargo protein, VSVG. We hypothesized that these effects are linked and might be explained by a cisternal-specific delay in cargo transport. In pulse chase experiments using a deconvolved, confocal line scanning approach to score the distribution of the tsO45 mutant of VSVG protein in Rab6 depleted cells, we found that anterograde transport at 32 °C, permissive conditions, through the Golgi apparatus was locally delayed, almost tenfold, between medial and trans Golgi cisterna. Cis to medial transport was nearly normal as was trans Golgi to TGN transport. TGN exit was unaffected by Rab6 depletion. These effects were the same with either of two siRNAs. Similar intra-Golgi transport delays were seen at 37 °C with RUSH VSVG or a RUSH GPI-anchored construct using a biotin pulse to release the marker proteins from the ER. Using 3D-SIM, a super resolution approach, we found that RUSH VSVG transport was delayed pre-trans Golgi. These visual approaches suggest a selective slowing of anterograde transport relative to 3 different marker proteins downstream of the trans Golgi. Using a biochemical approach, we found that the onset of VSVG endoglycosidase H resistance in Rab6 depleted cells was delayed. Depletion of neither Rab6A or Rab6A′ isoforms alone had any effect on anterograde transport through the Golgi suggesting that Rab6A and Rab6A′ act coordinately. Delayed cargo transport conditions correlate strongly with a proliferation of Golgi cisternae observed in earlier electron microscopy. Our results strongly indicate that Rab6 is selectively required for rapid anterograde transport from the medial to trans Golgi. We suggest that the observed correlation with localized cisternal proliferation fits best with a cisternal progression model of Golgi function.
Using epidemic simulators for monitoring an ongoing epidemic
Authors: Raghavan Mohan , Sridharan Kousik Sarathy , Mandayam Rangayyan Yashaswini
Published: October 2020

Prediction of infection trends, estimating the efficacy of contact tracing, testing or impact of influx of infected are of vital importance for administration during an ongoing epidemic. Most effective methods currently are empirical in nature and their relation to parameters of interest to administrators are not evident. We thus propose a modified SEIRD model that is capable of modeling effect of interventions and inward migrations on the progress of an epidemic. The tunable parameters of this model bear relevance to monitoring of an epidemic. This model was used to show that some of the commonly seen features of cumulative infections in real data can be explained by piecewise constant changes in interventions and population influx. We also show that the data of cumulative infections from twelve Indian states between mid March and mid April 2020 can be generated from the model by applying interventions according to a set of heuristic rules. Prediction for the next ten days based on this model, reproduced real data very well. In addition, our model also reproduced the time series of recoveries and deaths. Our work constitutes an important first step towards an effective dashboard for the monitoring of epidemic by the administration, especially in an Indian context.