bluuebunny/crossref_metadata_2025 huggingface dataset:
ASGR1 deficiency diverts lipids toward adipose tissue but results in liver damage during obesity
Authors: Svecla Monika , Da Dalt Lorenzo , Moregola Annalisa , Nour Jasmine , Baragetti Andrea , Uboldi Patrizia , Donetti Elena , Arnaboldi Lorenzo , Beretta Giangiacomo , Bonacina Fabrizia , Norata Giuseppe Danilo
Published: January 2024

Background

Asialoglycoprotein receptor 1 (ASGR1), primarily expressed on hepatocytes, promotes the clearance and the degradation of glycoproteins, including lipoproteins, from the circulation. In humans, loss-of-function variants of ASGR1 are associated with a favorable metabolic profile and reduced incidence of cardiovascular diseases. The molecular mechanisms by which ASGR1 could affect the onset of metabolic syndrome and obesity are unclear. Therefore, here we investigated the contribution of ASGR1 in the development of metabolic syndrome and obesity.

Methods

ASGR1 deficient mice (ASGR1−/−) were subjected to a high-fat diet (45% Kcal from fat) for 20 weeks. The systemic metabolic profile, hepatic and visceral adipose tissue were characterized for metabolic and structural alterations, as well as for immune cells infiltration.

Results

ASGR1−/− mice present a hypertrophic adipose tissue with 41% increase in fat accumulation in visceral adipose tissue (VAT), alongside with alteration in lipid metabolic pathways. Intriguingly, ASGR1−/− mice exhibit a comparable response to an acute glucose and insulin challenge in circulation, coupled with notably decreased in circulating cholesterol levels. Although the liver of ASGR1−/− have similar lipid accumulation to the WT mice, they present elevated levels of liver inflammation and a decrease in mitochondrial function.

Conclusion

ASGR1 deficiency impacts energetic homeostasis during obesity leading to improved plasma lipid levels but increased VAT lipid accumulation and liver damage.
Association of temporal change in body mass index with sudden cardiac arrest in diabetes mellitus
Authors: Jeong Joo Hee , Kim Yun Gi , Han Kyung-Do , Roh Seung-Young , Lee Hyoung Seok , Choi Yun Young , Shim Jaemin , Choi Jong-Il , Kim Young-Hoon
Published: January 2024

Background

Underweight imposes significant burden on cardiovascular outcomes in patients with diabetes mellitus. However, less is known about the impact of serial change in body weight status measured as body mass index (BMI) on the risk of sudden cardiac arrest (SCA). This study investigated the association between SCA and temporal change in BMI among patients with diabetes mellitus.

Methods

Based on Korean National Health Insurance Service database, participants with diabetes mellitus who underwent health examination between 2009 and 2012 and had prior health examination data (four years ago, 2005–2008) were retrospectively analyzed. BMI was measured at baseline (2005–2008) and 4-year follow-up health examination (2009–2012). Patients were classified in four groups according to the body weight status and its temporal change: sustained non-underweight, sustained underweight, previous underweight, and newly developed underweight. Primary outcome was defined as occurrence of SCA.

Results

A total of 1,355,746 patients with diabetes mellitus were included for analysis, and SCA occurred in 12,554 cases. SCA was most common in newly developed underweight (incidence rate = 4.45 per 1,000 person-years), followed by sustained underweight (incidence rate = 3.90), previous underweight (incidence rate = 3.03), and sustained non-underweight (incidence rate = 1.34). Adjustment of covariates resulted highest risk of SCA in sustained underweight (adjusted hazard ratio = 2.60, 95% confidence interval [2.25–3.00], sustained non-underweight as a reference), followed by newly developed underweight (2.42, [2.15–2.74]), and previous underweight (2.12, [1.77–2.53]).

Conclusions

In diabetes mellitus, sustained underweight as well as decrease in body weight during 4-year follow-up imposes substantial risk on SCA. Recovery from underweight over time had relatively lower, but yet increased risk of SCA. Both underweight and dynamic decrease in BMI can be associated with increased risk of SCA.
Joint angle estimation during shoulder abduction exercise using contactless technology
Authors: Barzegar Khanghah Ali , Fernie Geoff , Roshan Fekr Atena
Published: January 2024

Background

Tele-rehabilitation, also known as tele-rehab, uses communication technologies to provide rehabilitation services from a distance. The COVID-19 pandemic has highlighted the importance of tele-rehab, where the in-person visits declined and the demand for remote healthcare rises. Tele-rehab offers enhanced accessibility, convenience, cost-effectiveness, flexibility, care quality, continuity, and communication. However, the current systems are often not able to perform a comprehensive movement analysis. To address this, we propose and validate a novel approach using depth technology and skeleton tracking algorithms.

Methods

Our data involved 14 participants (8 females, 6 males) performing shoulder abduction exercises. We collected depth videos from an LiDAR camera and motion data from a Motion Capture (Mocap) system as our ground truth. The data were collected at distances of 2 m, 2.5 m, and 3.5 m from the LiDAR sensor for both arms. Our innovative approach integrates LiDAR with the Cubemos and Mediapipe skeleton tracking frameworks, enabling the assessment of 3D joint angles. We validated the system by comparing the estimated joint angles versus Mocap outputs. Personalized calibration was applied using various regression models to enhance the accuracy of the joint angle calculations.

Results

The Cubemos skeleton tracking system outperformed Mediapipe in joint angle estimation with higher accuracy and fewer errors. The proposed system showed a strong correlation with Mocap results, although some deviations were present due to noise. Precision decreased as the distance from the camera increased. Calibration significantly improved performance. Linear regression models consistently outperformed nonlinear models, especially at shorter distances.

Conclusion

This study showcases the potential of a marker-less system, to proficiently track body joints and upper-limb angles. Signals from the proposed system and the Mocap system exhibited robust correlation, with Mean Absolute Errors (MAEs) consistently below $$10^\circ$$

10
∘

. LiDAR’s depth feature enabled accurate computation of in-depth angles beyond the reach of traditional RGB cameras. Altogether, this emphasizes the depth-based system’s potential for precise joint tracking and angle calculation in tele-rehab applications.
Exosome-based delivery strategies for tumor therapy: an update on modification, loading, and clinical application
Authors: Yang Qian , Li Shisheng , Ou Haibo , Zhang Yuming , Zhu Gangcai , Li Shaohong , Lei Lanjie
Published: January 2024

Malignancy is a major public health problem and among the leading lethal diseases worldwide. Although the current tumor treatment methods have therapeutic effect to a certain extent, they still have some shortcomings such as poor water solubility, short half-life, local and systemic toxicity. Therefore, how to deliver therapeutic agent so as to realize safe and effective anti-tumor therapy become a problem urgently to be solved in this field. As a medium of information exchange and material transport between cells, exosomes are considered to be a promising drug delivery carrier due to their nano-size, good biocompatibility, natural targeting, and easy modification. In this review, we summarize recent advances in the isolation, identification, drug loading, and modification of exosomes as drug carriers for tumor therapy alongside their application in tumor therapy. Basic knowledge of exosomes, such as their biogenesis, sources, and characterization methods, is also introduced herein. In addition, challenges related to the use of exosomes as drug delivery vehicles are discussed, along with future trends. This review provides a scientific basis for the application of exosome delivery systems in oncological therapy.

Graphical Abstract
Association of spatial proximity to fixed-site syringe services programs with HCV serostatus and injection equipment sharing practices among people who inject drugs in rural New England, United States
Authors: Romo Eric , Stopka Thomas J. , Jesdale Bill M. , Wang Bo , Mazor Kathleen M. , Friedmann Peter D.
Published: January 2024

Background

Hepatitis C virus (HCV) disproportionately affects rural communities, where health services are geographically dispersed. It remains unknown whether proximity to a syringe services program (SSP) is associated with HCV infection among rural people who inject drugs (PWID).

Methods

Data are from a cross-sectional sample of adults who reported injecting drugs in the past 30 days recruited from rural counties in New Hampshire, Vermont, and Massachusetts (2018–2019). We calculated the road network distance between each participant’s address and the nearest fixed-site SSP, categorized as ≤ 1 mile, 1–3 miles, 3–10 miles, and > 10 miles. Staff performed HCV antibody tests and a survey assessed past 30-day injection equipment sharing practices: borrowing used syringes, borrowing other used injection equipment, and backloading. Mixed effects modified Poisson regression estimated prevalence ratios (aPR) and 95% confidence intervals (95% CI). Analyses were also stratified by means of transportation.

Results

Among 330 PWID, 25% lived ≤ 1 mile of the nearest SSP, 17% lived 1–3 miles of an SSP, 12% lived 3–10 miles of an SSP, and 46% lived > 10 miles from an SSP. In multivariable models, compared to PWID who lived within 1 mile of an SSP, those who lived 3 to 10 miles away had a higher prevalence of HCV seropositivity (aPR: 1.25, 95% CI 1.06–1.46), borrowing other used injection equipment (aPR: 1.23, 95% CI 1.04–1.46), and backloading (aPR: 1.48, 95% CI 1.17–1.88). Similar results were observed for PWID living > 10 miles from an SSP: aPR [HCV]: 1.19, 95% CI 1.01–1.40; aPR [borrowing other used equipment]:1.45, 95% CI 1.29–1.63; and aPR [backloading]: 1.59, 95% CI 1.13–2.24. Associations between living 1 to 3 miles of an SSP and each outcome did not reach statistical significance. When stratified by means of transportation, associations between distance to SSP and each outcome (except borrowing other used injection equipment) were only observed among PWID who traveled by other means (versus traveled by automobile).

Conclusions

Among PWID in rural New England, living farther from a fixed-site SSP was associated with a higher prevalence of HCV seropositivity, borrowing other used injection equipment, and backloading, reinforcing the need to increase SSP accessibility in rural areas. Means of transportation may modify this relationship.
Preclinical translational platform of neuroinflammatory disease biology relevant to neurodegenerative disease
Authors: Larson Kelley C. , Martens Lauren H. , Marconi Michael , Dejesus Christopher , Bruhn Suzanne , Miller Thomas A. , Tate Barbara , Levenson Jonathan M.
Published: January 2024

Neuroinflammation is a key driver of neurodegenerative disease, however the tools available to model this disease biology at the systems level are lacking. We describe a translational drug discovery platform based on organotypic culture of murine cortical brain slices that recapitulate disease-relevant neuroinflammatory biology. After an acute injury response, the brain slices assume a chronic neuroinflammatory state marked by transcriptomic profiles indicative of activation of microglia and astrocytes and loss of neuronal function. Microglia are necessary for manifestation of this neuroinflammation, as depletion of microglia prior to isolation of the brain slices prevents both activation of astrocytes and robust loss of synaptic function genes. The transcriptomic pattern of neuroinflammation in the mouse platform is present in published datasets derived from patients with amyotrophic lateral sclerosis, Huntington’s disease, and frontotemporal dementia. Pharmacological utility of the platform was validated by demonstrating reversal of microglial activation and the overall transcriptomic signature with transforming growth factor-β. Additional anti-inflammatory targets were screened and inhibitors of glucocorticoid receptors, COX-2, dihydrofolate reductase, and NLRP3 inflammasome all failed to reverse the neuroinflammatory signature. Bioinformatics analysis of the neuroinflammatory signature identified protein tyrosine phosphatase non-receptor type 11 (PTPN11/SHP2) as a potential target. Three structurally distinct inhibitors of PTPN11 (RMC-4550, TN0155, IACS-13909) reversed the neuroinflammatory disease signature. Collectively, these results highlight the utility of this novel neuroinflammatory platform for facilitating identification and validation of targets for neuroinflammatory neurodegenerative disease drug discovery.
Rare germline mutation and MSH2-&MSH6 + expression in a double primary carcinoma of colorectal carcinoma and endometrial carcinoma: a case report
Authors: Zhang Tiansong , Huang Xiaoqiang , Liu Wenjie , Ling Xiulan , Su Zhenping , Huang Mengwei , Che Shuanlong
Published: January 2024

Background

Multiple primary malignancies are rare in cancer patients, and risk factors may include genetics, viral infection, smoking, radiation, and other environmental factors. Lynch syndrome (LS) is the most prevalent form of hereditary predisposition to double primary colorectal and endometrial cancer in females. LS, also known as hereditary nonpolyposis colorectal cancer (HNPCC), is a common autosomal dominant condition. Pathogenic germline variants in the DNA mismatch repair (MMR) genes, namely MLH1, MSH2, MSH6, and PMS2, and less frequently, deletions in the 3’ end of EPCAM cause LS. It manifested itself as loss of MMR nuclear tumor staining (MMR protein deficient, dMMR).

Case presentation

This case study describes a double primary carcinoma in a 49-year-old female. In June 2022, the patient was diagnosed with highly to moderately differentiated endometrioid adenocarcinoma. The patient’s mother died of esophageal cancer at age 50, and the father died of undefined reasons at age 70. Immunohistochemical stainings found ER (++), PR (++), P53 (+), MSH2 (-), MSH6 (+), MLH1 (+), and PMS2 (+). MMR gene sequencing was performed on endometrial tumor and peripheral blood samples from this patient. The patient carried two pathogenic somatic mutations in the endometrial tumor, MSH6 c.3261dupC (p.Phe1088LeufsTer5) and MSH2 c.445_448dup (p.Val150fs), in addition to a rare germline mutation MSH6 c.133G > C (p.Gly45Arg). Two years ago, the patient was diagnosed with moderately differentiated adenocarcinoma in the left-half colon. Immunohistochemical stainings found MSH2(-), MSH6(+), MLH1(+), and PMS2(+) (data not shown).

Conclusions

In the case of a patient with double primary EC and CRC, a careful evaluation of the IHC and the genetic data was presented. The patient carried rare compound heterozygous variants, a germline missense mutation, and a somatic frameshift mutation of MSH6, combined with a novel somatic null variant of MSH2. Our study broadened the variant spectrum of double primary cancer and provided insight into the molecular basis for abnormal MSH2 protein loss and double primary carcinoma.
Multifaceted surgical approach of combined thoracoretroperitoneal incision and midline abdominal incision for a secondary aortoenteric fistula
Authors: Minami Tomoyuki , Kojima Takahiro , Yabu Naoto , Yamazaki Ichiya , Saito Aya
Published: January 2024

Background

We report a one-stage surgery to the case of secondary aortoenteric fistula (sAEF) after prosthetic reconstruction of abdominal aortic aneurysm, by multifaceted approach.

Case presentation

A 63-year-old male was admitted to our unit under diagnosed of sAEF after prosthetic reconstruction of abdominal aortic aneurysm, and a pseudoaneurysm of thoracoabdominal aorta due to infection. The patient underwent emergency operation. Firstly, we placed the patient in a modified right lateral decubitus position and performed thoracoabdominal aortic replacement with retroperitoneal approach by thoracoretroperitoneal incision which combined thoracotomy and pararectal incision, and secondly, we changed to a supine position and performed closure of the duodenal fistula and omental flap transposition by midline abdominal incision. The patient was doing well without complications.

Conclusions

A one-stage, multifaceted surgical approach covering both prosthetic reconstruction of thoracoabdominal aorta and closure of sAEF with omentopexy is reasonable and useful strategy.
Short and mid-term outcomes of valve-sparing, aortic root reimplantation (David’s procedure)
Authors: Salehi Omran Abbas , Aeen Ali , Nayebirad Sepehr , Vakili-Basir Ahmad , Najafi Mohammad Sadeq , Mohseni-Badalabadi Reza , Shirani Shapour , Zoroufian Arezou , Jalali Arash , Mostafanejad Fatemeh Alsadat , Sahebjam Mohammad
Published: January 2024

Background

In the current study, we aimed to report the short- and mid-term outcomes of patients undergoing valve-sparing aortic root reimplantation (VSARR) and our center’s experience with the procedure.

Methods

Forty patients with aortic root aneurysms underwent VSARR at our center from 2010 until 2022. We retrospectively reviewed the medical records of these patients and extracted the relevant data. After carefully examining the aortic valve, the surgeon decided to perform Bentall or David’s procedure during the operation.

Results

The study population comprised 31 (77.5%) men and nine (22.5%) women, with a mean age of 55.35 ± 15.40. One patient developed hemodynamic instability post-surgery in the hospital and died from multi-organ failure. Another patient had severe AI in the intraoperative echocardiography, and aortic valve replacement with a prosthetic graft was performed during the same operation. In pre-operation echocardiography, 25 (62.5%) patients had severe, nine (22.5%) had moderate, and six (15%) had mild AI. In the in-hospital post-operation follow-up echo, AI was improved, and no patients had severe AI (P < 0.001). Only eight patients had moderate AI in post-one-year follow-up echo exams, while the rest had mild AI.

Conclusion

David’s procedure showed excellent mid-term results in our center, with only one in-hospital mortality.
An elevated urinary albumin-to-creatinine ratio increases the risk of incident cardia-cerebrovascular disease in individuals with type 2 diabetes
Authors: Tao Jie , Sang Dasen , Zhang Xinxin , Liu Xin , Wang Guodong , Chen Shuohua , Wu Shouling , Geng Wei
Published: January 2024

Aims

We aimed to explore the associations between urine albumin-to-creatinine ratio (uACR) and cardia-cerebrovascular disease (CVD) in Chinese population with type 2 diabetes(T2D).

Methods

We included 8975 participants with T2D but free of prevalent CVD (including myocardial infarction, ischemic and hemorrhagic stroke) at baseline from Kailuan study who were assessed with uACR between 2014 and 2016. The participants were divided into three groups based on their baseline uACR: normal (< 3 mg/mmol), microalbuminuria (3–30 mg/mmol), and macroalbuminuria (≥ 30 mg/mmol). Cox regression models and restricted cubic spline were used to evaluate the hazard ratios (HRs) and 95% confidence intervals (CIs) of incident CVD. The area under the receiver operating characteristic curve (AUC), net reclassification improvement (NRI), and integrated discrimination improvement (IDI) were used to see if incorporating uACR into existing models could improve performance.

Results

During a median follow-up of 4.05 years, 560 participants developed first CVD event (6.24%). After adjustment for potential confounders, participants with microalbuminuria had higher risks of CVD compared with normal uACR, with HRs of 1.57(95% CI 1.04–2.37) for myocardial infarction, 1.24(95% CI 1.00–1.54) for ischemic stroke,1.62(95% CI 0.73–3.61) for hemorrhagic stroke, and 1.30(95% CI 1.07–1.57) for total CVD. The risks gradually attenuated with uACR increase, with HRs of 2.86(95% CI 1.63–5.00) for myocardial infarction, 2.46(95% CI 1.83–3.30) for ischemic stroke, 4.69(95% CI 1.72–12.78) for hemorrhagic stroke, and 2.42(95% CI 1.85–3.15) for total CVD in macroalbuminuria. The addition of uACR to established CVD risk models improved the CVD risk prediction efficacy.

Conclusions

Increasing uACR, even below the normal range, is an independent risk factor for new-onset CVD in T2D population. Furthermore, uACR could improve the risk prediction for CVD among community based T2D patients.
When sequential use of mepolizumab and dupilumab in a severe atopic eosinophilic asthmatic questions the role of eosinophils in mediating the clinical expression of the disease: a case report
Authors: Sabbe M. , Schleich F. , Janssens P. , Louis R.
Published: January 2024

Background

The advent of biologics has resulted in major progress in the treatment of severe T2 high asthmatics. There are currently several classes of biologics approved for severe asthma including anti-immunoglobulin E, anti-interleukin-5/interleukin 5R, anti-interleukin 4/interleukin 13R, and anti-thymic stromal lymphopoietin.

Case presentations

Here we report the case of a 55-year-old Caucasian man with severe eosinophilic atopic asthma, who sequentially benefited from a treatment with mepolizumab, an anti-interleukin-5 monoclonal antibody, followed by treatment with dupilumab, an anti-interleukin-4/interleukin-13R antibody, the switch being justified by a flare-up of dermatitis while on mepolizumab. Overall, the patient has been followed for 72 months, including 42 months on mepolizumab and 30 months on dupilumab. Close monitoring of exacerbations, asthma control, lung function, asthma quality of life, and biomarkers shows that both biologics reduced asthma exacerbation and provided an improvement in asthma control and quality of life, with the patient achieving remission after 30 months on dupilumab. However, the effects of the two biologics on the biomarkers were very different, with mepolizumab controlling eosinophilic inflammation and dupilumab reducing serum immunoglobulin E and fractional exhaled nitric oxide levels.

Conclusion

The originality of this case resides in the description of clinical status and biomarker evolution after a sequential use of mepolizumab and dupilumab in a severe atopic eosinophilic asthmatic. It shows that mepolizumab reduces exacerbation and improves asthma control by curbing eosinophilic inflammation whereas dupilumab provides asthma remission without controlling airway eosinophilic inflammation.
Visceral leishmaniasis misdiagnosed as an upper respiratory infection and iron-deficiency anemia in a 20-month-old male patient: a case report
Authors: Chakhunashvili Davit G. , Chakhunashvili Konstantine , Kvirkvelia Eka
Published: January 2024

Background

Visceral Leishmaniasis should be suspected in every patient with a history of splenomegaly, fever, and pancytopenia. It is one of the most dangerous forms of infection and prompt recognition is the key to positive outcome.

Case presentation

A 20-month-old Caucasian male patient was brought to our hospital as an outpatient with the complaint of persistent fever, which did not improve with empiric antibiotic treatment (> 96 hour after the initial dose). The antibiotic treatment had been prescribed by primary care physician at polyclinic, who also referred the patient to hematologist due to anemia, who prescribed iron supplement. Despite multiple subspecialist visits, bicytopenia was, unfortunately, left unidentified. Upon physical examination no specific signs were detected, however, spleen seemed slightly enlarged. Patient was admitted to the hospital for further work-up, management and evaluation. Abdominal ultrasound, complete blood count and c-reactive protein had been ordered. Hematologist and infectionist were involved, both advised to run serology for Epstein-Barr Virus and Visceral Leishmaniasis. The latter was positive; therefore, patient was transferred to the specialized clinic for specific management.

Conclusion

Both in endemic and non-endemic areas the awareness about VL should be increased among the medical professionals. We also recommend that our colleagues take the same approach when dealing with bicytopenia and fever, just as with pancytopenia and fever. The medical community should make sure that none of the cases of fever and pancytopenia are overlooked, especially if we have hepatomegaly and/or splenomegaly.
Capsular gene distribution and RAPD typing of Streptococcus agalactiae isolated from pregnant women
Authors: Zakerifar Mona , Goli Hamid Reza , Kaboosi Hami , Rahmani Zahra , Peyravii Ghadikolaii Fatemeh
Published: January 2024

Streptococcus agalactiae has different virulence factors, from which the capsule has the most significant role in the pathogenesis of this organism. We aimed to investigate the distribution of more prevalent capsular genes among different Random Amplified Polymorphic DNA (RAPD) types of S. agalactiae isolated from pregnant women. A total of 106 isolates were collected from 420 vaginal and rectal swabs obtained from pregnant women. The specimens were transferred using Todd Hewitt Broth and were cultured on a blood agar containing antibiotics. The S. agalactiae isolates were identified by the standard microbiological and biochemical tests. The genomic DNAs of S. agalactiae isolates were extracted using an extraction kit. Then, the PCR method was used to detection of the capsular genes. Moreover, The RAPD PCR was used to genotyping of the isolates. The colonization rate of the pregnant women was 25.23%, and there was a statistically significant correlation between the weeks of gestation and the probability of colonization (p-value < 0.05). Also, 31 (29.24%) and 18 (16.98%) pregnant women had a history of abortion and membrane rupture, respectively. In addition, 20 (18.86%), 32 (30.18%), 4 (3.77%), and 6 (5.66%) isolates carried genes encoding capsular types Ia, Ib, III, and V, respectively. None isolates had the type II capsular gene, and other 44 isolates were non-typeable. Nine clones (clusters) of S. agalactiae were observed in the present study with 70% similarity, and 53 different types were identified among the isolates. Except for capsular types III and V that belonged to clones 3, 5, 7, and 9, other capsular types were detected in different RAPD types. We found that the capsular types Ib and Ia were predominant among pregnant women in this area, indicating their significance for vaccine designation. Also, our isolates showed a lower genotypic diversity in RAPD typing. This may be due to the same sources of most isolates.
A Bayesian workflow for the analysis and reporting of international large-scale assessments: a case study using the OECD teaching and learning international survey
Authors: Kaplan David , Harra Kjorte
Published: January 2024

This paper aims to showcase the value of implementing a Bayesian framework to analyze and report results from international large-scale assessments and provide guidance to users who want to analyse ILSA data using this approach. The motivation for this paper stems from the recognition that Bayesian statistical inference is fast becoming a popular methodological framework for the analysis of educational data generally, and large-scale assessments more specifically. The paper argues that Bayesian statistical methods can provide a more nuanced analysis of results of policy relevance compared to standard frequentist approaches commonly found in large-scale assessment reports. The data utilized for this paper comes from the Teaching and Learning International Survey (TALIS). The paper provides steps in implementing a Bayesian analysis and proposes a workflow that can be applied not only to TALIS but to large-scale assessments in general. The paper closes with a discussion of other Bayesian approaches to international large-scale assessment data, in particularly for predictive modeling.
Time-dependent modeling of slow-slip events along the Nankai Trough subduction zone, Japan, within the 2018–2023 period
Authors: Ozawa Shinzaburo , Muneakane Hiroshi , Suito Hisashi
Published: January 2024

According to a 2013 report by the Earthquake Research Committee of Japan, it was estimated that the probability of the occurrence of the next Nankai earthquake within the next three decades is 70–80%. Therefore, to realize hazard estimation, it is crucial to estimate the state of the plate interface in the Nankai Trough subduction zone. In this study, we focused on a transient from 2018 in the subduction zone of the Nankai Trough, southwest Japan, detected by the Global Navigation Satellite System (GNSS) network. Thereafter, we estimated the spatiotemporal evolution of the slip at the plate interface by subjecting the GNSS position time series to time-dependent inversion. The results obtained showed that a long-term slow-slip event (l-SSE) possibly occurred on the Kii Peninsula in 2020. The Kii-Channel l-SSE (Mw 6.3), with an irregular recurrence interval, was observed from 2019 to 2022. Additionally, the Central Shikoku l-SSE (Mw 6.5) was observed from 2019 to 2023, and there appeared to be a correlation between the Central Shikoku l-SSE and the northwestern Shikoku short-term slow-slip event (s-SSE). l-SSE occurred from July 2018 to August 2019 in the northern Hyuga-nada and Bungo channel, and in late 2018, another l-SSE occurred in southern Hyuga-nada before the May 2019 Hyuga-nada earthquake. Further, after the 2018–2019 events, the southern Hyuga-nada l-SSE occurred from mid-2020 to early 2021 and in January 2023 and is still ongoing, consistent with the expected recurrence interval. The seemingly transient slip off the Ohsumi Peninsula, detected after the Mw 5.7 earthquake of October 2022, continued until July 2023 with Mw 6.1. Furthermore, after the 2019 Tanegashima earthquake, an l-SSE occurred for approximately 4 years. The moment magnitude (Mw) of the afterslip of the Tanegashima earthquake was estimated to be 6.7, larger than that of the main shock (Mw 6.4). We also noted that the transient slip off Tanegashima included four possible s-SSEs that occurred in 2020, 2021, 2022, and 2023. Therefore, the sporadic aseismic slips along the Nankai Trough changed the stress state of the areas neighboring the aseismic slip zones in favor of the interplate slip.

Graphical Abstract
Validation of a discovery MI 4-ring model according to the NEMA NU 2-2018 standards: from Monte Carlo simulations to clinical-like reconstructions
Authors: Merlet Antoine , Presles Benoît , Su Kuan-Hao , Salvadori Julien , Sayah Farzam , Jozi Hanieh , Cochet Alexandre , Vrigneaud Jean-Marc
Published: January 2024

Background

We propose a comprehensive evaluation of a Discovery MI 4-ring (DMI) model, using a Monte Carlo simulator (GATE) and a clinical reconstruction software package (PET toolbox). The following performance characteristics were compared with actual measurements according to NEMA NU 2-2018 guidelines: system sensitivity, count losses and scatter fraction (SF), coincidence time resolution (CTR), spatial resolution (SR), and image quality (IQ). For SR and IQ tests, reconstruction of time-of-flight (TOF) simulated data was performed using the manufacturer’s reconstruction software.

Results

Simulated prompt, random, true, scatter and noise equivalent count rates closely matched the experimental rates with maximum relative differences of 1.6%, 5.3%, 7.8%, 6.6%, and 16.5%, respectively, in a clinical range of less than 10 kBq/mL. A 3.6% maximum relative difference was found between experimental and simulated sensitivities. The simulated spatial resolution was better than the experimental one. Simulated image quality metrics were relatively close to the experimental results.

Conclusions

The current model is able to reproduce the behaviour of the DMI count rates in the clinical range and generate clinical-like images with a reasonable match in terms of contrast and noise.
Heterotopic pregnancy after a single embryo transfer with successful perinatal outcome: case report and literature review
Authors: Chen Wanqi , Qi Jingyi
Published: January 2024

A heterotopic pregnancy is a rare and serious pathological pregnancy. In this paper, we report a rare case of heterotopic pregnancy and perform a literature review. A 30-year-old patient with a history of left adnexectomy presented with persistent lower abdominal pain and hemorrhagic shock after single embryo transfer. Emergency laparoscopic exploration revealed a ruptured mass in the right isthmus of the fallopian tube, for which right salpingectomy was performed. After anti-inflammatory treatment and fetal preservation, the intrauterine pregnancy progressed smoothly, and a healthy baby was delivered at 39 weeks gestation. In this case, the patient’s heterotopic pregnancy was possibly due to a natural pregnancy caused by sexual intercourse during treatment, so we recommend that sexual intercourse be avoided during transfer cycles.
Four year evaluation of a parent advisory group to support a research program for knowledge translation in child health
Authors: Hartling Lisa , Elliott Sarah A. , Mabbott Annie , Leung Julie , Shearer Kathleen , Smith Chrissy , Scott Shannon D.
Published: January 2024

Background

In 2016, we developed a pediatric parent advisory group to inform our research program which creates innovative knowledge translation (KT) tools for parents on priority topics related to acute childhood illness. We implemented a mixed methods strategy to evaluate the experiences of group members. The purpose of this paper is to present the findings from parent evaluations over four years and to discuss our experiences collaborating with the group over a multi-year period.

Methods

We conducted year-end surveys and interviews of group members to understand parents’ perceptions of their experiences, group management, researcher interaction, and other outcomes of advisory group participation from 2018 to 2021. We applied a mixed methods approach, collecting and analyzing both quantitative (survey) and qualitative (survey/interview) data. Survey data were analyzed by term using descriptive statistics (i.e., frequencies, percentages). Open-ended survey responses were analyzed by conventional content analysis. Interview data were analysed thematically.

Results

Year-end survey response rates and interview participation varied over the years. Responses to evaluation questions were generally positive and most improved over time. Results prompted changes to improve P-PAG operations, such as changes to location of meetings, communications about the group’s purpose, offering sufficient context for discussion items, and providing feedback about how members’ input was used. Themes identified from the qualitative data related to the importance of certain aspects of group functioning, positive views of the group’s current management, and potential areas for improvement. Parents regularly expressed a desire for more diversity in the group’s membership and an interest in hearing more about how the research program’s activities fit into the broader healthcare system and their impacts on health outcomes.

Conclusions

Our experience in establishing, managing, and evaluating a parent advisory group over many years has resulted in valuable insights regarding patient engagement in health research and sustaining an advisory group over time. We have learned that an intentional and iterative approach with regular evaluations and responsive changes has been essential for fostering meaningful engagement. Significant resources are required to maintain the group; in turn, the group has made substantial and diverse contributions to the research program and its outputs.
Disparities in healthcare access experienced by Hispanic chronic kidney disease patients: a cross-sectional analysis
Authors: Ashrafi Sadia Anjum , Alam Rifat Binte , Kraay Alicia , Ogunjesa Babatope Ayokunle , Schwingel Andiara
Published: January 2024

Background

Chronic kidney disease (CKD) is a public health concern, and the disease disproportionately affects Hispanics. Improved healthcare access for Hispanic CKD patients can reduce the disease burden. This study assesses the healthcare access disparities experienced by Hispanic CKD patients compared to Whites.

Methods

We analyzed three National Health and Nutrition Examination Survey (NHANES) datasets for 2013–2014, 2015–2016, and 2017–2018. The primary predictor variable was race, and the outcome variable was three domains of healthcare access: insurance status, having any routine place for healthcare, and having any health visits in the past year. Chi-square tests and unadjusted and adjusted multivariate logistic regressions were conducted. The models were adjusted for age, education, income, and CKD stages and were weighted to account for the sampling strategy.

Results

The sample size was 1864 CKD patients from three two-year cycles of NHANES datasets (2013–2014, 2015–2016, and 2017–2018). The final adjusted model found that Hispanic CKD patients were more likely to be uninsured (OR: 2.52, CI 1.66–3.83) and have no routine place for healthcare (OR: 1.68, CI 1.03–2.75) than White CKD patients, but did not have differences in healthcare visits in the past year.

Conclusions

Hispanic CKD patients have limited healthcare access compared to White populations showing existing care access disparities experienced by them. Improved programs and policies are required to enhance kidney health among Hispanics and promote equity in CKD.
Integrative single-cell analysis: dissecting CD8 + memory cell roles in LUAD and COVID-19 via eQTLs and Mendelian Randomization
Authors: Wu Jintao , Mao Xiaocheng , Liu Xiaohua , Mao Junying , Yang Xianxin , zhou Xiangwu , Tianzhu Lu , Ji Yulong , Li Zhao , Xu Huijuan
Published: January 2024

Lung adenocarcinoma exhibits high incidence and mortality rates, presenting a significant health concern. Concurrently, the COVID-19 pandemic has emerged as a grave global public health challenge. Existing literature suggests that T cells, pivotal components of cellular immunity, are integral to both antiviral and antitumor responses. Yet, the nuanced alterations and consequent functions of T cells across diverse disease states have not been comprehensively elucidated. We gathered transcriptomic data of peripheral blood mononuclear cells from lung adenocarcinoma patients, COVID-19 patients, and healthy controls. We followed a standardized analytical approach for quality assurance, batch effect adjustments, and preliminary data processing. We discerned distinct T cell subsets and conducted differential gene expression analysis. Potential key genes and pathways were inferred from GO and Pathway enrichment analyses. Additionally, we implemented Mendelian randomization to probe the potential links between pivotal genes and lung adenocarcinoma susceptibility. Our findings underscored a notable reduction in mature CD8 + central memory T cells in both lung adenocarcinoma and COVID-19 cohorts relative to the control group. Notably, the downregulation of specific genes, such as TRGV9, could impede the immunological efficacy of CD8 + T cells. Comprehensive multi-omics assessment highlighted genetic aberrations in genes, including TRGV9, correlating with heightened lung adenocarcinoma risk. Through rigorous single-cell transcriptomic analyses, this investigation meticulously delineated variations in T cell subsets across different pathological states and extrapolated key regulatory genes via an integrated multi-omics approach, establishing a robust groundwork for future functional inquiries. This study furnishes valuable perspectives into the etiology of multifaceted diseases and augments the progression of precision medicine.
Development guidelines for individual digital study assistants in higher education
Authors: König Claudia M. , Karrenbauer Christin , Breitner Michael H.
Published: January 2024

Increasing student numbers, heterogeneity and individual biographies lead to a growing need for personalized support. To meet these challenges, an Individual Digital Study Assistant (IDSA) provides features to help students improve their self-regulation and organizational skills to achieve individual study goals. Based on qualitative expert interviews, a quantitative student survey, and current literature we derived requirements for an IDSA. Based on them, we designed, developed, and implemented a first IDSA prototype for higher education institutions (HEI). We continuously evaluated the prototype within different workshops and analyzed the usage data to improve it further in three enhanced prototypes. Based on this iterative process, we derived guidelines for an IDSA design and development. Accordingly, the framework, project management, content, team selection, team development, team communication, marketing, and student habits are important to consider. The guidelines advance the knowledge base of IDSA in HEI and guide and support practitioners in the design, development, and implementation of IDSA in HEI.
Screening and characterization of bioactive compounds from two epiphytic microlichen and evaluation of their in vitro antioxidant activity
Authors: Pradhan Shubham , Dash Satyabrata , Sahoo Bijayananda , Parida Sabyasachy , Rath Biswajit
Published: January 2024

Background

Lichens in symbiosis produce a wide range of primary and secondary fine compounds in extreme environmental conditions that have a broad range of biological properties as well as antioxidant potential and can be used in future pharmaceuticals as a natural source of antioxidant molecules.

Results

The two microlichen species collected are identified based on morphological and molecular techniques; further studies are carried out by analyzing phytochemicals (FTIR, GC MS), and antioxidant assays are evaluated. The non-enzymatic antioxidant activity is evaluated by DPPH and FRAP assays. The methanol extract of both lichens showed virtuous DPPH scavenge with IC50 of P. nitida (125.76 ± 0.023 µg/ml) and G. scripta IC50 (176.90 ± 0.058 µg/ml). FRAP activity was prominent in the methalonic extract. The enzymatic antioxidant activity is observed by SOD and catalase activity. The cytosolic (Cu–Zn-SOD and Fe-SOD) and mitochondrial SOD (Mn-SOD) are detected in lichens, though P. nitida shows mitochondrial Mn-SOD and cytosolic Cu–Zn-SOD and Fe-SOD, whereas G. scripta has a single cytosolic Cu–Zn-SOD; however, two isoforms of catalase were reported. GC–MS analysis screened bioactive metabolites such as phenols, Quinons, heterocyclic compounds, benzofurans, fatty acids, pyrans, carboxylic acid, aliphatic aldehydes, organic alcohol, fluorinated aliphatic substances, ketones, terpenes and fatty alcohols in P. nitida, whereas, in G. scripta screened fatty acids, alcohols, hydrocarbons, carbonyl compounds, polyols, terpenes, glycosides, phenols, and sugar alcohols detected in the chromatogram peak. FTIR analysis revealed functional groups like Alcohols, Amines, Amides, Alkanes, Aldehydes, Carboxylic acid, Alkynes, Esters, Ketones, Anhydrides, Acid chlorides, Alkenes, Aromatic compounds, Nitro compounds, Alkyl and Aryl Halides in both lichens.

Conclusions

The results obtained in the present study proved that P. nitida and G. scripta have promising antioxidant activity owing to the presence of polyphenols and terpenes, as evidenced by DPPH and FRAP assay along with enzymatic analysis (SOD and CAT). Thus both the lichens may be used as natural sources of new bioactive molecules having pharmaceutical interest.
Comprehensive preclinical studies on the bioactivity of Orbignya phalerata Mart. (Babassu) and its derived products: a systematic review
Authors: Debia Nicole , Castro Isione Oliveira , Sousa Ana Beatriz Santana , de Oliveira Victor Alves , Negreiros Helber Alves , Ferreira Paulo Michel Pinheiro , de Castro e Sousa João Marcelo
Published: January 2024

Background

Babassu (Orbignya phalerata Mart.) is a palm tree well distributed in Latin America, whose fruit has a mesocarp and kernel used for human feeding, and empirically related to the treatment of gastritis, vaginitis, and wound healing.

Main body of the abstract

The activities attributed to babassu can guide new research on health applications and, for this reason, this study aimed to report in vitro and in vivo biological activities of O. phalerata constituents through a systematic review. Searches terms were applied in five world databases and the data from the publications were collected according to PICOS criteria, including the fruit component, concentration/dose, time of exposure, and comparative groups. All outcomes were reported and the most relevant outcomes were described by a narrative synthesis and a risk of bias assessment. A total of 28 in vitro (n = 15) and in vivo (n = 11) studies were included, and two showed both experimental designs. The studies were heterogeneous, with the predominance of metabolic analysis, wound and peptic ulcer healing, besides in vivo toxicity, among others. For in vitro analysis, antioxidant tests, cell viability and antimicrobial activity predominated. All in vivo ones used rodents. Meanwhile, tumor and non-tumor cell lines, bacteria strains, Leishmania amazonensis, Artemia salina, and antioxidant reactions were considered for in vitro protocols.

Short conclusion

The most frequent applications included mesocarp and kernel in a wide range of extracts, emulsions, and concentrations. Their low in vitro lethality and cytotoxicity, and no acute toxicity in vivo open possibilities for the development of long-term toxicity assays with repeated doses in rodents and interventions in clinical trials.

Graphic abstract
A statistical overview of the research history of the superfamily Buprestoidea (Coleoptera)
Authors: JENDEK EDUARD
Published: January 2024

A review of selected bibliographic, nomenclatural and taxonomic aspects of the study of Buprestoidea (families Schizopodidae and Buprestoidae) is presented from the Linne‘s time to the recent (1758-2023). All data are represented by diagrams. The number of documented taxonomic publications related to Buprestoidea is 11,629 with the annual largest of 192 publications appearing in 2007. The most prolific author in history is Obenberger with 455 publications. Available genus-group names include 256 invalid names and 777 valid names; the latter consist of 520 generic and 257 subgeneric names. Most genus-group names start with P, C or A and the four oldest valid ones are Buprestis, Trachys, Aphanisticus and Agrilus. Most genus-group names were proposed in 1864 (38 names) and in the period 1918-1937 (170 names). Names of 129 accepted genera or subgenera have invalid names (synonyms). A total of 137 authors contributed to generic-group names with the arithmetic mean 7.5 names per author. A total of 23,389 available species-group names exist in the superfamily Buprestoidea among which 6,551 (28%) are invalid and 16,838 (72%) are valid; the latter consist of 15,921 (94.6%) specific names and 917 (5.4%) subspecific names. The most frequent species-group name is strandi with 80 occurrences. Most species-group names begins with c, s or a. Most frequent species-group name 3-letter prefix is sub- (234 occurrences). The highest number of species-group names was proposed in 1924 (721 names) and in the period 1918-1937 (6,124 names). The global arithmetic mean of currently valid to invalid species-group names per year is 69.8%. A total of 660 authors contributed to the species-group names with the arithmetic mean is 35.4 names per author. Obenberger with 5,395 species-group names is by far the most prolific author. The subfamily Agrilinae has most genera (205, 39.4% of valid genera of Buprestoidea). Two tribes with the largest number of genera are also from subfamily Agrilinae: Coraebini (131 genera) and Agrilini (44 genera). Among 520 accepted genera of Buprestoidea, 71 (13.6%) have subgenera. The superfamily Buprestoidea contains 15,921 accepted species, among which seven belong to the family Schizopodidae and the rest to the family Buprestidae. Almost half (7,282, 45.8%) of all species of Buprestoidea is from the three agriline tribes: Agrilini, Tracheini and Coraebini. The most speciose genus in Buprestoidea, and in the whole of Animalia, is Agrilus (3,431 species), followed by Sphenoptera (1,002). Chrysochroa fulminans (Fabricius, 1787) is the jewel beetle species with the highest number of recognized subspecies (18).