bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Effects of Freeze-Dried Sourdoughs with Lactiplantibacillus pentosus 129 and Limosilactobacillus fermentum 139 on the Quality of Long-Fermentation Bread
Authors: Santos Joanderson Gama , de Souza Evandro Leite , de Souza Couto Marcus Vinícius , Rodrigues Tatiana Zanella , de Medeiros Ana Regina Simplício , de Magalhães Cordeiro Angela Maria Tribuzy , Lima Marcos dos Santos , de Oliveira Maria Elieidy Gomes , da Costa Lima Maiara , de Araújo Noádia Priscilla Rodrigues , Gonçalves Ingrid Conceição Dantas , Garcia Estefânia Fernandes
Published: June 2024

Sourdough production is a complex fermentation process. Natural sourdough fermentation without standardization causes great variability in microbial communities and derived products. Starter cultures have emerged as alternatives to natural fermentation processes, which could improve bakery quality and produce bioactive compounds. This study aimed to evaluate the impacts of freeze-drying on the production and viability of sourdoughs with Lactiplantibacillus pentosus 129 (Lp) and Limosilactobacillus fermentum 139 (Lf), as well as their effects on the quality of long-fermentation bread. These strains were selected based on their better performance considering acidification and exopolysaccharide production capacity. Sourdough with Lp and Lf were propagated until the 10th day, when physicochemical and microbiological parameters were determined. The produced sourdoughs were freeze-dried, and bread samples were produced. The freeze-drying process resulted in high survival rates and few impacts on the metabolic activity of Lp and Lf until 60 days of storage. Incorporating Lp and Lf improved the microbiological and physicochemical properties of sourdough and long-fermentation breads. Tested freeze-dried sourdoughs led to reduced bread aging (higher specific volume and decreased starch retrogradation) and increased digestibility. The results show the potential of the freeze-dried sourdoughs produced with Lp and Lf as innovative strategies for standardizing production protocols for the bakery industry, especially for producing long-term fermentation bread.
Inhibitory Potential of Bifidobacterium longum FB1-1 Cell-Free Supernatant against Carbapenem-Resistant Klebsiella pneumoniae Drug Resistance Spread
Authors: Wang Jing , Fan Dan-Cai , Wang Rui-Shan , Chang Yu , Ji Xue-Meng , Li Xin-Yang , Zhang Yan , Liu Jing-Min , Wang Shuo , Wang Jin
Published: June 2024

The widespread dissemination of carbapenem-resistant Klebsiella pneumoniae (CRKP) and its drug resistance transfer poses a global public health threat. While previous studies outlined CRKP’s drug resistance mechanism, there is limited research on strategies inhibiting CRKP drug resistance spread. This study investigates the potential of Bifidobacterium longum (B. longum) FB1-1, a probiotic, in curbing the spread of drug resistance among CRKP by evaluating its cell-free supernatant (CFS) for antibacterial activity. Evaluating the inhibitory effect of FB1-1 CFS on CRKP drug resistance spread involved analyzing its impact on drug resistance and virulence gene expression; drug resistance plasmid transfer FB1-1 CFS exhibited an MIC range of 125 μL/mL against CRKP. After eight hours of co-culture, CFS achieved a 96% and 100% sterilization rate at two and four times the MIC, respectively. At sub-inhibitory concentrations (1/2× MIC), FB1-1 CFS reduced the expression of the bla_KPC gene, which is pivotal for carbapenem resistance, by up to 62.13% across different CRKP strains. Additionally, it markedly suppressed the expression of the uge gene, a key virulence factor, by up to 91%, and the fim_H gene, essential for bacterial adhesion, by up to 53.4%. Our study primarily focuses on determining the inhibitory effect of FB1-1 CFS on CRKP strains harboring the bla_KPC gene, which is a critical resistance determinant in CRKP. Furthermore, FB1-1 CFS demonstrated the ability to inhibit the transfer of drug resistance plasmids among CRKP strains, thus limiting the horizontal spread of resistance genes. This study highlights FB1-1 CFS's inhibitory effect on CRKP drug resistance spread, particularly in strains carrying the bla_KPC gene, thus offering a novel idea and theoretical foundation for developing antibacterial drugs targeting CRKP resistance.
Diverse Behaviors of Feldspar Grains during Sandstone Diagenesis: Example from the Xujiahe Formation in the Western Sichuan Basin, China
Authors: Zhong Yijiang , Huang Keke , Zou Qing , Zhang Shupeng , Ye Liming
Published: June 2024

Feldspar alteration is among the most important processes in clastic rocks during diagenesis, but uncertainty remains about the factors that control feldspar diagenesis under subsurface conditions. Hence, the Upper Triassic Xujiahe formation of the Western Sichuan Basin were examined by an integrated petrographic, mineralogical and geochemical approach to unravel the causes and effects of feldspar diagenesis, with implication for mass transfer and openness of the geochemical system. The sandstones at various depths demonstrate three distinct, separate diagenetic behaviors of detrital feldspar within a single formation including (1) the complete dissolution of both plagioclase and K-feldspar in the upper member; (2) conservation of abundant detrital feldspar grains with minor albitization or overgrowths within the lower member of depths greater than 5 km; and (3) complete disappearance of K-feldspar within the uppermost horizons of the lower member, while plagioclase have survived in significant amounts. The exceptional disappearance of K-feldspar is the result of selective dissolution of K-feldspar during burial, accompanied by illite cementation and substantial K transfer at a scale of tens of meters. It is apparent that the clay diagenesis in the overlying mudstones, rather than porewater chemistry, is the major control of the reactivity of K-feldspar in adjacent deeply buried sandstones.
Characterization of Limestone Surface Impurities and Resulting Quicklime Quality
Authors: Sandström Karin , Carlborg Markus , Eriksson Matias , Broström Markus
Published: June 2024

Quicklime, rich in CaO(s), is generated by calcining limestone at high temperatures. Parallel-flow regenerative lime kilns are the most energy-effective industrial method available today. To prevent major disruptions in such kilns, a high raw material quality is necessary. Under some conditions, impurity-enriched material may adhere to limestone pebbles and enter the kiln. In this study, limestone and corresponding quicklime were analyzed to evaluate the extent and composition of surface impurities and assess the effect on quicklime product quality, here defined as free CaO. This was performed by sampling and analyzing limestone, quarry clay, laboratory-produced quicklime, and industrially produced quicklime with XRF, SEM/EDX, and XRD; interpretations were supported by thermodynamic equilibrium calculations. In the laboratory-produced quicklime, the surface impurities reacted with calcium forming Larnite, Gehlenite, Åkermanite and Merwinite, reducing the quicklime quality. The results showed that the limestone surface layer comprised 1.2 wt.-% of the total mass but possessed 4 wt.-% of the total impurities. The effect on industrially produced quicklime quality was lower; this indicated that the limestone surface impurities were removed while the material moved through the kiln. Multicomponent chemical equilibrium calculations showed that the quarry clay was expected to be fully melted at 1170 °C, possibly leading to operational problems.
Bulk and Surface Characterization of Distinct Hematite Morphology: Implications for Wettability and Flotation Response
Authors: Faustino Lívia , McFadzean Belinda , Gouvêa Junior José , Leal Filho Laurindo
Published: June 2024

To understand why hematite of different genesis behave diversely in flotation systems, this study assesses the flotation response at pH 5 of bulk (morphology, texture, Crystal Preferential Orientation (CPO)) plus interfacial (surface area, zeta potential, immersion enthalpy, contact angle, induction time) characteristics of species formed under distinct metamorphic conditions: low-strain deformation (Hematite-1) versus high-strain deformation (Hematite-2). Hematite-2 (predominantly composed of specular and lamellar morphologies) shows (001) CPO and exhibits fewer Fe sites on its surface that undergo doubly coordinated Fe-OH when exposed to moisture. This results in a less reactive surface associated with a less ordered adsorbed water layer than Hematite-1, which is predominantly composed of granular and sinuous hematite. Those characteristics lead to a naturally hydrophobic behavior characterized by the exothermic energy below the Critical Immersion Enthalpy (−∆Himm < 200 mJ/m2), lower values of zeta potential due to attenuated dissociation of Fe-OH(surf), lower induction time (47 ms vs. 128 ms), higher contact angle (39° vs. 13°), and higher flotation recovery (21% vs. 12%) than Hematite-1.
LightSub: Unobtrusive Subtitles with Reduced Information and Decreased Eye Movement
Authors: Nishi Yuki , Nakamura Yugo , Fukushima Shogo , Arakawa Yutaka
Published: June 2024

Subtitles play a crucial role in facilitating the understanding of visual content when watching films and television programs. In this study, we propose a method for presenting subtitles in a way that considers cognitive load when viewing video content in a non-native language. Subtitles are generally displayed at the bottom of the screen, which causes frequent eye focus switching between subtitles and video, increasing the cognitive load. In our proposed method, we focused on the position, display time, and amount of information contained in the subtitles to reduce the cognitive load and to avoid disturbing the viewer’s concentration. We conducted two experiments to investigate the effects of our proposed subtitle method on gaze distribution, comprehension, and cognitive load during English-language video viewing. Twelve non-native English-speaking subjects participated in the first experiment. The results show that participants’ gazes were more focused around the center of the screen when using our proposed subtitles compared to regular subtitles. Comprehension levels recorded using LightSub were similar, but slightly inferior to those recorded using regular subtitles. However, it was confirmed that most of the participants were viewing the video with a higher cognitive load using the proposed subtitle method. In the second experiment, we investigated subtitles considering connected speech form in English with 18 non-native English speakers. The results revealed that the proposed method, considering connected speech form, demonstrated an improvement in cognitive load during video viewing but it remained higher than that of regular subtitles.
The Mediating Effect of Coping Strategies and Emotion Regulation in the Relationship between Impulsivity, Metacognition, and Eating Disorders
Authors: Estévez Ana , Momeñe Janire , Macía Laura , Iruarrizaga Iciar , Olave Leticia , Aonso-Diego Gema
Published: June 2024

Background. Risk factors for eating disorders are multifaceted and complex, so it is crucial to elucidate the role of executive functions, including impulsivity and metacognition, and coping strategies in the severity of eating behaviors. The study aims were threefold: (1) to analyze gender differences in impulsivity, metacognition, coping strategies, emotion regulation, and eating disorders; (2) to examine the correlation between the study variables; and (3) to test the mediating role of coping and emotion-regulation strategies in the relationship between metacognition, impulsivity, and eating disorders. Methods. A total of 1076 participants (Mage = 21.78, SD = 5.10; 77.7% women) completed a set of questionnaires. Two mediation analyses were conducted to test the mediating role of coping strategies, including emotion regulation, in the relationship between executive functions (i.e., impulsivity and metacognition) and eating disorders. Results. Women displayed higher coping strategies, specifically emotional expression, wishful thinking, and social support, whereas men presented greater social withdrawal. Mediational analyses showed a significant association between impulsivity, metacognition, and eating disorders, whose relationship was partially mediated by coping strategies and mainly by emotion regulation. Conclusion. Interventions based on coping strategies and emotion regulation could be a feasible and effective option to deal with eating disorders among the young population.
Green-Synthesized Characterization, Antioxidant and Antibacterial Applications of CtAC/MNPs-Ag Nanocomposites
Authors: Baran Ayşe , Ertaş Erdal , Baran Mehmet Fırat , Eftekhari Aziz , Gunes Zübeyir , Keskin Cumali , Usanov Sergey A. , Khalilov Rovshan
Published: June 2024

The emergence of antibiotic resistance, caused by the improper use of antibiotics, is a significant challenge in combating infectious diseases, leading to millions of annual fatalities. The occurrence of antimicrobial side effects catalyzes the investigation of novel antimicrobial compounds and sources of drugs. Consequently, the research on biological activity that is conducted on plants, plant extracts, and compounds that are produced from plant components is of utmost significance. In this study, CtAC/MNPs were obtained by the reaction of activated carbon (AC) obtained from the fruits of the Celtis tournefortii (Ct) plant and magnetic nanoparticles (MNPs), and a CtAC/MNPs-Ag nanocomposite was synthesized by the reduction in silver ions added to the reaction. The synthesized CtAC/MNPs and CtAC/MNPs-Ag nanocomposites were analyzed spectroscopically (FTIR, XRD), microscopically (SEM, EDX), optically (DLS), electrochemically (zeta potential) and magnetically (VSM). The antibacterial activities of CtAC/MNPs and CtAC/MNPs-Ag nanocomposites against S. aureus and E. coli were investigated by microdilution method using minimal inhibitory concentration (MIC) and disk diffusion methods. Antioxidant activity study, including total phenolic content and DPPH and cuprac assays, revealed the remarkable effect of the CtAC/MNPs-Ag nanocomposite. This study has the advantages of obtaining CtAC/MNPs and CtAC/MNPs-Ag nanocomposites in a short time without requiring energy, and most importantly, the reaction takes place without using any toxic substances. In addition, according to the data obtained in the study, the CtAC/MNPs-Ag nanocomposite is thought to shed light on biomedical research.
Solubility, pH-Solubility Profile, pH-Rate Profile, and Kinetic Stability of the Tyrosine Kinase Inhibitor, Alectinib
Authors: Madlool Dheyaa Tohma , Al-Ani Israa , Ata Tha’er , Dayyih Wael Abu
Published: June 2024

Alectinib HCl (ALBHCl) is a tyrosine kinase inhibitor used for non-small cell lung carcinoma (NSCLC). The aim of this study is to unlock some of the physicochemical properties of ALBHCL that serve as a database for any future studies. A solubility study of ALBHCL was performed in different solvents. Also, photostability was tested in the solution and solid states, and the order of reaction and rate constant were calculated. In addition to the pH solubility relation, the pH-rate relation at different temperatures was also studied, and the profiles were constructed. A solubility study was also performed in different media for the purpose of optimizing suitable sink conditions for the in vitro dissolution testing of solid dosage forms. Solubility tests in multiple solvents and pH conditions revealed that the highest solubility was in DMSO, methanol, and chloroform, with acidic media yielding the maximum solubility but degrading at rather low pH levels. ALBHCL proved unstable at high temperatures and under light exposure, with varying stability across different pH levels. The optimal dissolution media for in vitro oral dosage form evaluation were determined, achieving sink conditions at pH levels of 6.8 and 4.5 with specific additives. This study enhances the existing database on ALBHCL’s physicochemical properties, emphasizing the importance of pH optimization in pharmaceutical processes and providing valuable insights into its pharmaceutical application.
Novel Ultrasound-Responsive Amyloid Formulation
Authors: Ismail Maytham , Kanapathipillai Mathumai
Published: June 2024

Amyloid aggregates have attracted significant interest in regard to diverse biomedical applications, particularly in the field of drug delivery. Here, we report novel amyloid aggregates based on a 12-amino-acid peptide from the amyloidogenic region of the receptor-interacting kinase 3 (RIP3) protein and a thermoresponsive triblock copolymer, namely, Pluronic F127 (RIP3/F127). Physicochemical characterization was performed to determine the aggregation size, morphology, and stimuli-responsive properties. The potential of the aggregates as a drug depot was assessed in lung cancer cells, using Doxorubicin (Dox) as a model drug. The results show that RIP3 and RIP3/F127 exhibit amyloidogenic properties. Further, the RIP3/F127 amyloids exhibited significant ultrasound-responsive properties compared to amyloid aggregates without Pluronic F127. Moreover, the RIP3/F127/Dox amyloid formulations that were subjected to ultrasound treatment exhibited greater toxicity to lung cancer cells compared to that of Dox alone at equal concentrations. Overall, the results from this proof-of-concept study show that amyloidogenic peptide aggregates with stimuli-responsive properties can be utilized as efficient drug delivery depots.
One-Step Synthesis, Crystallography, and Acute Toxicity of Two Boron–Carbohydrate Adducts That Induce Sedation in Mice
Authors: Cordova-Chávez Ricardo Ivan , Trujillo-Ferrara José G. , Padilla-Martínez Itzia I. , González-Espinosa Héctor , Abad-García Antonio , Farfán-García Eunice D. , Ortega-Camarillo Clara , Contreras-Ramos Alejandra , Soriano-Ursúa Marvin A.
Published: June 2024

Boronic acids form diester bonds with cis-hydroxyl groups in carbohydrates. The formation of these adducts could impair the physical and chemical properties of precursors, even their biological activity. Two carbohydrate derivatives from d-fructose and d-arabinose and phenylboronic acid were synthesized in a straightforward one-step procedure and chemically characterized via spectroscopy and X-ray diffraction crystallography. Additionally, an acute toxicity test was performed to determine their lethal dose 50 (LD50) values by using Lorke’s method. Analytical chemistry assays confirmed the formation of adducts by the generation of diester bonds with the β-d-pyranose of carbohydrates, including signals corresponding to the formation of new bonds, such as the stretching of B–O bonds. NMR spectra yielded information about the stereoselectivity in the synthesis reaction: Just one signal was found in the range for the anomeric carbon in the 13C NMR spectra of both adducts. The acute toxicity tests showed that the LD50 value for both compounds was 1265 mg/kg, while the effective dose 50 (ED50) for sedation was 531 mg/kg. However, differences were found in the onset and lapse of sedation. For example, the arabinose derivative induced sedation for more than 48 h at 600 mg/kg, while the fructose derivative induced sedation for less than 6 h at the same dose without the death of the mice. Thus, we report for the first time two boron-containing carbohydrate derivatives inducing sedation after intraperitoneal administration. They are bioactive and highly safe agents. Further biological evaluation is desirable to explore their medical applications.
Recent Progress of Multifunctional Molecular Probes for Triple-Negative Breast Cancer Theranostics
Authors: Zhao Deyi , Li Zhe , Ji Ding-Kun , Xia Qian
Published: June 2024

Breast cancer (BC) poses a significant threat to women’s health, with triple-negative breast cancer (TNBC) representing one of the most challenging and aggressive subtypes due to the lack of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression. Traditional TNBC treatments often encounter issues such as low drug efficiency, limited tumor enrichment, and substantial side effects. Therefore, it is crucial to explore novel diagnostic and treatment systems for TNBC. Multifunctional molecular probes (MMPs), which integrate target recognition as well as diagnostic and therapeutic functions, introduce advanced molecular tools for TNBC theranostics. Using an MMP system, molecular drugs can be precisely delivered to the tumor site through a targeted ligand. Real-time dynamic monitoring of drug release achieved using imaging technology allows for the evaluation of drug enrichment at the tumor site. This approach enables accurate drug release, thereby improving the therapeutic effect. Therefore, this review summarizes the recent advancements in MMPs for TNBC theranostics, encompassing the design and synthesis of MMPs as well as their applications in the field of TNBC theranostics.
Development, Optimization, and Clinical Relevance of Lactoferrin Delivery Systems: A Focus on Ocular Delivery
Authors: Ponzini Erika , Astolfi Gloria , Grandori Rita , Tavazzi Silvia , Versura Piera
Published: June 2024

Lactoferrin (Lf), a multifunctional protein found abundantly in secretions, including tears, plays a crucial role in ocular health through its antimicrobial, immunoregulatory, anti-inflammatory, and antioxidant activities. Advanced delivery systems are desirable to fully leverage its therapeutic potential in treating ocular diseases. The process of Lf quantification for diagnostic purposes underscores the importance of developing reliable, cost-effective detection methods, ranging from conventional techniques to advanced nano-based sensors. Despite the ease and non-invasiveness of topical administration for ocular surface diseases, challenges such as rapid drug elimination necessitate innovations, such as Lf-loaded contact lenses and biodegradable polymeric nanocapsules, to enhance drug stability and bioavailability. Furthermore, overcoming ocular barriers for the treatment of posterior segment disease calls for nano-formulations. The scope of this review is to underline the advancements in nanotechnology-based Lf delivery methods, emphasizing the pivotal role of multidisciplinary approaches and cross-field strategies in improving ocular drug delivery and achieving better therapeutic outcomes for a wide spectrum of eye conditions.
Advances in 2,3-Dimethylmaleic Anhydride (DMMA)-Modified Nanocarriers in Drug Delivery Systems
Authors: Wan Dong , Wu Yanan , Liu Yujun , Liu Yonghui , Pan Jie
Published: June 2024

Cancer represents a significant threat to human health. The cells and tissues within the microenvironment of solid tumors exhibit complex and abnormal properties in comparison to healthy tissues. The efficacy of nanomedicines is inhibited by the presence of substantial and complex physical barriers in the tumor tissue. The latest generation of intelligent drug delivery systems, particularly nanomedicines capable of charge reversal, have shown promise in addressing this issue. These systems can transform their charge from negative to positive upon reaching the tumor site, thereby enhancing tumor penetration via transcytosis and promoting cell internalization by interacting with the negatively charged cell membranes. The modification of nanocarriers with 2,3-dimethylmaleic anhydride (DMMA) and its derivatives, which are responsive to weak acid stimulation, represents a significant advance in the field of charge-reversal nanomedicines. This review provides a comprehensive examination of the recent insights into DMMA-modified nanocarriers in drug delivery systems, with a particular focus on their potential in targeted therapeutics. It also discusses the synthesis of DMMA derivatives and their role in charge reversal, shell detachment, size shift, and ligand reactivation mechanisms, offering the prospect of a tailored, next-generation therapeutic approach to overcome the diverse challenges associated with cancer therapy.
Development of neffy, an Epinephrine Nasal Spray, for Severe Allergic Reactions
Authors: Ellis Anne K. , Casale Thomas B. , Kaliner Michael , Oppenheimer John , Spergel Jonathan M. , Fleischer David M. , Bernstein David , Camargo Carlos A. , Lowenthal Richard , Tanimoto Sarina
Published: June 2024

Epinephrine autoinjectors (EAIs) are used for the treatment of severe allergic reactions in a community setting; however, their utility is limited by low prescription fulfillment rates, failure to carry, and failure to use due to fear of needles. Given that delayed administration of epinephrine is associated with increased morbidity/mortality, there has been a growing interest in developing needle-free, easy-to-use delivery devices. neffy (epinephrine nasal spray) consists of three Food and Drug Administration (FDA)-approved components: epinephrine, Intravail A3 (absorption enhancer), and a Unit Dose Spray (UDS). neffy’s development pathway was established in conjunction with the FDA and the European Medicines Agency and included multiple clinical trials to evaluate pharmacokinetic and pharmacodynamic responses under a variety of conditions, such as self-administration and allergic and infectious rhinitis, as well as an animal anaphylaxis model of severe hypotension, where neffy demonstrated a pharmacokinetic profile that is within the range of approved injection products and a pharmacodynamic response that is as good or better than injections. The increased pulse rate (PR) and blood pressure (BP) observed even one minute following the administration of neffy confirm the activation of α and β adrenergic receptors, which are the key components of epinephrine’s mechanism of action. The results suggest that neffy will provide a safe and effective needle-free option for the treatment of severe allergic reactions, including anaphylaxis.
Generalized-Mode Averaging Technique for Wrapped Phase
Authors: Tang Zhan , Liu Fengwei , Wu Yongqian
Published: June 2024

In this paper, a generalized-mode phase averaging technique is proposed to suppress air turbulence and random noise in optical shop testing. This approach eliminates the need to repeatedly unwrap and thus greatly improves processing efficiency. By removing the random tilt component of the wrapped phase, a set of wrapped phases that are corrupted by random vibrations can be unified into the same mode, some of which obey a circular distribution. Therefore, the circular mean technique can be used for wrapped phase averaging; only one unwrapping process is required for a set of wrapped phases. A criterion based on maximum likelihood estimation is proposed to determine scenarios for the use of this method. The effects of noise and air disturbances on this method are discussed. Finally, the effectiveness of the method is demonstrated by simulations and experiments.
Alkaloid Accumulation and Distribution within the Capsules of Two Opium Poppy (Papaver somniferum L.) Varieties
Authors: Májer Péter , Németh Éva Zámboriné
Published: June 2024

The goal of this study was to clarify the role of capsule size and morphology in the alkaloid yield of poppy. In 2023, two industrial varieties from large-scale cultivation were investigated. Three classes of capsule size (large, medium, and small) and four organelles (wall, placenta, disc, and thalamus) of the seedless capsule were studied for their mass proportions and alkaloid contents. In ‘Meara’, large capsules had 41% lower total alkaloid levels than smaller ones. In ‘Morgana’, there was no difference in total alkaloids between size groups, but large capsules had higher contents of codeine and thebaine. Among the four organelles, the wall represented the largest mass in both varieties (60–67%), while the disc and the thalamus gave the lowest proportions (below 9%). In the variety ’Meara’, the highest alkaloid contents appeared in the wall (2.69% d.w.), followed by the placenta, and the other two parts. ‘Morgana’ accumulated the highest alkaloid content (3.72% d.w.) in the placenta. Morphine follows the trend of the total content, while codeine and thebaine may differ. Accurate information on the accumulation of alkaloids in the generative organs may contribute to increasing effectiveness in target-oriented breeding and optimization of cultivation with an appropriate choice of variety.
Investigating the Potential of Essential Oils from Citrus reticulata Leaves in Mitigating Memory Decline and Oxidative Stress in the Scopolamine-Treated Zebrafish Model
Authors: Brinza Ion , Boiangiu Razvan Stefan , Honceriu Iasmina , Abd-Alkhalek Ahmed M. , Eldahshan Omayma A. , Dumitru Gabriela , Hritcu Lucian , Todirascu-Ciornea Elena
Published: June 2024

Petitgrain essential oil (PGEO) is derived from the water distillation process on mandarin (Citrus reticulata) leaves. The chemical constituents of PGEO were analyzed by gas chromatography/mass spectrometry (GC/MS) method which revealed the presence of six compounds (100%). The major peaks were for methyl-N-methyl anthranilate (89.93%) and γ-terpinene (6.25%). Over 19 days, zebrafish (Tubingen strain) received PGEO (25, 150, and 300 μL/L) before induction of cognitive impairment with scopolamine immersion (SCOP, 100 μM). Anxiety-like behavior and memory of the zebrafish were assessed by a novel tank diving test (NTT), Y-maze test, and novel object recognition test (NOR). Additionally, the activity of acetylcholinesterase (AChE) and the extent of the brain’s oxidative stress were explored. In conjunction, in silico forecasts were used to determine the pharmacokinetic properties of the principal compounds discovered in PGEO, employing platforms such as SwissADME, Molininspiration, and pKCSM. The findings provided evidence that PGEO possesses the capability to enhance memory by AChE inhibition, alleviate SCOP-induced anxiety during behavioral tasks, and diminish brain oxidative stress.
Functional Characterization of the Soybean Glycine max Actin Depolymerization Factor GmADF13 for Plant Resistance to Drought Stress
Authors: Wang Deying , Du Mengxue , Lyu Peng , Li Jingyu , Meng Huiran , Liu Xinxin , Shi Mengmeng , Gong Yujie , Sha Qi , Men Qingmei , Li Xiaofei , Sun Yongwang , Guo Shangjing
Published: June 2024

Abiotic stress significantly affects plant growth and has devastating effects on crop production. Drought stress is one of the main abiotic stressors. Actin is a major component of the cytoskeleton, and actin-depolymerizing factors (ADFs) are conserved actin-binding proteins in eukaryotes that play critical roles in plant responses to various stresses. In this study, we found that GmADF13, an ADF gene from the soybean Glycine max, showed drastic upregulation under drought stress. Subcellular localization experiments in tobacco epidermal cells and tobacco protoplasts showed that GmADF13 was localized in the nucleus and cytoplasm. We characterized its biological function in transgenic Arabidopsis and hairy root composite soybean plants. Arabidopsis plants transformed with GmADF13 displayed a more robust drought tolerance than wild-type plants, including having a higher seed germination rate, longer roots, and healthy leaves under drought conditions. Similarly, GmADF13-overexpressing (OE) soybean plants generated via the Agrobacterium rhizogenes-mediated transformation of the hairy roots showed an improved drought tolerance. Leaves from OE plants showed higher relative water, chlorophyll, and proline contents, had a higher antioxidant enzyme activity, and had decreased malondialdehyde, hydrogen peroxide, and superoxide anion levels compared to those of control plants. Furthermore, under drought stress, GmADF13 OE activated the transcription of several drought-stress-related genes, such as GmbZIP1, GmDREB1A, GmDREB2, GmWRKY13, and GmANK114. Thus, GmADF13 is a positive regulator of the drought stress response, and it may play an essential role in plant growth under drought stress conditions. These results provide new insights into the functional elucidation of soybean ADFs. They may be helpful for breeding new soybean cultivars with a strong drought tolerance and further understanding how ADFs help plants adapt to abiotic stress.
Ammonium Tetrakis(pentafluorophenyl)borate: Preparation and Application in Olefin Coordination Polymerization as the Cocatalyst Compound
Authors: Wei Yiming , Qu Shuzhang , Li Xinwei , Chen Jian , Wen Zhao , Li Qian , Wang Wei
Published: June 2024

Metallocene catalysts have attracted much attention from academia and industry for their excellent catalytic activity in the field of olefin polymerization. Cocatalysts play a key role in metallocene catalytic systems, which can not only affect the overall catalytic activity, but also have an obvious influence on the structure and properties of the polymer. Although methylaluminoxane (MAO) is currently the most widely used cocatalyst, its price increases the production cost of polyolefin materials. Ammonium tetrakis(pentafluorophenyl)borate has shown excellent performance in polymerization, being one of the best substitutes for the traditional cocatalyst MAO. Compared with the main catalyst, whose composition and structure are relatively complex, the research on cocatalyst is very limited. This review mainly introduces the research history, preparation methods, and application progress in polymerization of ammonium tetrakis(pentafluorophenyl)borate, deepening our understanding of the role of cocatalyst in polymerization, with the hope of inspiring brand-new thinking on improving and enhancing the overall performance of catalyst systems.
Performance of Oriented Strand Board Made of Heat-Treated Bamboo (Dendrocalamus asper (Schult.) Backer) Strands
Authors: Maulana Sena , Suwanda Astri Aulia , Murda Rio Ardiansyah , Antov Petar , Komariah Rahma Nur , Maulana Muhammad Iqbal , Augustina Sarah , Lee Seng Hua , Mahardika Melbi , Rianjanu Aditya , Taher Tarmizi , Kristak Lubos , Bindar Yazid , Iswanto Apri Heri , Lubis Muhammad Adly Rahandi
Published: June 2024

This study aimed to analyze the effect of pre-heat treatment on bamboo strand properties and its impact on the properties of the resulting bamboo-oriented strand board (BOSB). Giant bamboo (Dendrocalamus asper (Schult.) Backer) with a density of 0.53 g cm−3 was converted into bamboo strands. These strands were pre-heat-treated at 140 and 160 °C for a duration of 1, 2, and 3 h. Changes in the chemical composition of the strand due to subsequent treatment were assessed. Fourier-transform infrared spectroscopy (FTIR) and X-Ray diffraction analysis (XRD) were used to determine the changes in the chemical composition of bamboo strands. The BOSB panels were produced with a target density of 0.7 g cm−3. The manufacturing of the BOSB was conducted in three layers with a ratio of 25:50:25, bonded with phenol-formaldehyde resin. The physical and mechanical properties of the laboratory-fabricated BOSB were tested in compliance with the criteria given in JIS A 5908 standards. Comparisons were made against OSB CSA 0437.0 Grade O-1 commercial standard. The pre-heat treatment led to chemical alterations within the material when set at 140 and 160 °C for 1 to 3 hours (h). FTIR spectral analysis demonstrated that longer exposure and higher temperatures resulted in fewer functional groups within the bamboo strands. The increased temperature and duration of pre-heat treatment enhanced the crystallinity index (CI). The dimensional stability and mechanical properties of the composites were improved significantly as hemicellulose and extractive content were reduced. This study demonstrated that the pre-heat treatment of bamboo strands at a temperature of 160 °C for a duration of 1 h was an adequate approach for heat modification and fabrication of BOSB panels with acceptable properties according to OSB CSA 0437.0 Grade O-1 commercial standard.
Optimization of Starch–Tannin Adhesives for Solid Wood Gluing
Authors: Magnabosco Annalisa , Kulyk Illya , Avancini Maurizio , Šket Primož , Eckardt Jonas , Cesprini Emanuele , Marinello Francesco , Tondi Gianluca
Published: June 2024

Bio-based solutions for solid timber gluing have always been a very sensitive topic in wood technology. In this work, we optimize the gluing conditions of a starch–tannin formulation, which allows high performance in dry conditions and resistance to water dipping for 3 h, allowing for the D2 classification to be reached according to EN 204. It was observed that the starch–tannin formulations enhanced their performance by increasing the heating temperature, achieving satisfactory results at 140 °C for 13 min. The proportion of polyphenols in the mixture enhances the water resistance but is only tolerated until 20–30%. In particular, the addition of 10% tannin–hexamine enhances the water-resistant properties of starch for both quebracho and chestnut extract. The application of the jet of cold atmospheric plasma allows for good results with more viscous formulations, increasing their penetration in wood. Solid-state 13C-NMR analysis was also performed, and the spectroscopic information suggests establishing a coordination complex between starch and tannin.
Mechanical Characteristics of Sandwich Structures with 3D-Printed Bio-Inspired Gyroid Structure Core and Carbon Fiber-Reinforced Polymer Laminate Face-Sheet
Authors: Junaedi Harri , Abd El-baky Marwa A. , Awd Allah Mahmoud M. , Sebaey Tamer A.
Published: June 2024

The gyroid structure is a bio-inspired structure that was discovered in butterfly wings. The geometric design of the gyroid structure in butterfly wings offers a unique combination of strength and flexibility. This study investigated sandwich panels consisting of a 3D-printed gyroid structure core and carbon fiber-reinforced polymer (CFRP) facing skin. A filament fused fabrication 3D printer machine was used to print the gyroid cores with three different relative densities, namely 10%, 15%, and 20%. Polylactic acid (PLA) was used as the printing material for the gyroid. The gyroid structure was then sandwiched and joined by an epoxy resin between CFRP laminates. Polyurethane foam (PUF) was filled into the gyroid core to fill the cavity on the core for another set of samples. Flexural and compression tests were performed on the samples to investigate the mechanical behavior of the sandwiches. Moreover, the two-parameter Weibull distribution was used to evaluate the results statistically. As a result, the sandwich-specific facing stress and core shear strength from the three-point bending test of the composites increased with the increase in sandwich density. Core density controls the flexural characteristics of the sandwich. Adding PUF improves the deflection at the maximum stress and the sustained load after fracture of the sandwich. Compression strength, modulus, and energy absorbed by gyroid core sandwiches and their specific properties are higher than the PUF-filled gyroid core sandwiches at equal sandwich density.
Analysis of Fatigue Crack Nucleation in Double-Network Hydrogels
Authors: Gao Shan , Jiang Liying
Published: June 2024

Hydrogel-based devices commonly have a high demand for material durability when subjected to prolonged or cyclic loads. To extend their service life, it is crucial to have a deep understanding of the fatigue mechanisms of hydrogels. It is well-known that double-network (DN) hydrogels are characterized by high strength and toughness and are thus recognized as a promising candidate under load-bearing conditions. However, the existing studies in the literature mainly focus on their resistant capability to fatigue crack growth, while the underlying mechanisms of fatigue crack nucleation are still inconclusive. This work aims to bridge this knowledge gap by formulating a fatigue life predictor for DN hydrogels within the framework of configurational mechanics to elucidate the underlying mechanisms governing fatigue crack nucleation. The fatigue life predictor for DN hydrogels is derived from the configurational stress by incorporating the corresponding constitutive models and the thermodynamic evolution laws for microdamage mechanisms and material viscoelasticity. With the developed fatigue predictor, the effect of the microdamage mechanism on fatigue is elucidated, i.e., the internal damage of the sacrificial network can improve the fatigue life of DN hydrogels. The fatigue life predictor is also adopted to evaluate the effects of some other factors on the fatigue crack nucleation, such as the loading rate, pre-stretching treatment, and water diffusion, identifying feasible loading profiles that could improve material durability. Overall, the theoretical framework and the modeling results in this work are expected to shed light on unveiling the fatigue mechanisms of DN hydrogels and advance the design of hydrogel-based devices.
Sustainable Management of Manufacturing Processes: A Literature Review
Authors: Tiuncika Lasma , Bormane Santa
Published: June 2024

Industrial economic activity is one of the primary causes of environmental degradation and a source of issues related to social inequality. Nevertheless, research has shown that the application of the sustainable entrepreneurship model can significantly improve environmental and social conditions while maintaining continuous and sustainable economic growth. Despite that, statistics show relatively low engagement in sustainable entrepreneurship, potentially due to a lack of expertise among entrepreneurs. Due to the low engagement, available sustainability performance metrics are not a relevant measurement tool. Thus, the purpose of this study is to identify a set of criteria that evaluate expertise levels in sustainable entrepreneurship within the manufacturing industry. Through the application of monographic and secondary data analysis methods, the authors carried out a literature review. The findings indicated that sustainable management is a key component to the triple-bottom-line (TBL) framework that is used as a conceptual basis for sustainable entrepreneurship. Additionally, the authors identified 19 criteria of sustainable entrepreneurship within four dimensions of sustainability (environmental, social, economic, and management). Despite the limitations, with a more refined literature review, the criteria could be applied as a comprehensive resource to estimate expertise in sustainable entrepreneurship in future studies on small and medium enterprises (SMEs).
Representations of the Ottoman Empire in the West: Abdülhamid II’s Portrait in the French Press
Authors: Argun Selim , Dursun Hatice Rumeysa
Published: June 2024

This article examines representations of Ottoman Sultan Abdülhamid II in the French press. Analyzing these representations will help one to better understand the Western perceptions of the Ottoman Empire and the Orient. This study is based on the analysis of selected French newspapers such as L’Illustration, Le Journal Illustré, Le Monde Illustré, L’Univers Illustré and Le Petit Journal, as well as other secondary sources (articles and books). Using a critical discourse analysis, this study aims to comprehend how the West, particularly the French press, positioned Abdülhamid II and the Ottoman Empire and how it evolved over time. The analysis of the coverage of Abdülhamid II’s accession to power and, afterwards, his portrait as a Sultan demonstrates not only the struggle of the European powers (especially France and Britain) to gain supremacy over the Ottoman Empire but also the orientalist discourse of the French press with regard to Abdülhamid II. While the coverage of Abdülhamid II’s enthronement ceremony contains both positive and negative representations of the Sultan and the Ottoman Empire, a negative approach was adopted mostly in the coverage of his portrait after his accession to power as Abdülhamid II, as he adopted a policy of balance vis-à-vis the European powers. Overall, the French representations of Abdülhamid II and the Ottoman Empire shed light on the rivalries among the European powers over the Ottoman Empire at the turn of the 19th century.
Transitions in Patristic Cosmology: From Cosmophobia to Universe-(Re)Making
Authors: Costache Doru
Published: June 2024

The field of Patristics, or early Christian and Mediaeval Studies, traditionally works along the lines of historical and literary criticism. But this method is not always useful, especially when it comes to complex objects and circumstances. No wonder the current trend of replacing it, more often than not, by interdisciplinary frameworks. The article begins accordingly by reviewing three interdisciplinary frameworks, namely, the “socio-historical method”, “Deep Time”, and archaeological theorist Roland Fletcher’s “transitions”, highlighting their suitability for a comprehensive approach to Patristic cosmology. Here, cosmology should not be taken in the narrow sense of contemporary science. It means both a way of representing reality—a worldview—and a way of inhabiting the world. The present article analyses the evolution of the early Christian and mediaeval perception of the environment and the cosmos in Greek sources, pointing to successive transitions from apprehension (cosmophobia) to a keen interest in understanding nature to the thought that holiness represents a universe-(re)making agency. It addresses relevant historical and social circumstances, but proposes that the above transitions were triggered by internal or existential factors as well, and not only external, thus complementing Fletcher’s outline, which focuses upon external catalysts, such as economy and technology.
Sustaining Algeria’s Retirement System in the Population Aging Context: Could a Contribution Cap Strategy Work?
Authors: Flici Farid , Dominguez-Fabian Inmaculada
Published: June 2024

Previous research predicts an increasing financial deficit in Algeria’s PAYG retirement system, mainly due to rapid population aging, and parametric adjustments will be insufficient to alleviate this imbalance. Mitigating the effects of population aging will necessitate further intervention. In this work, we analyze how capping contributed salaries can help to mitigate the effects of population aging on the retirement system. Under generous Pay-As-You-Go schemes, promised pension payouts far exceed contributions. Thus, restricting contributions is expected to reduce the burden of future benefits by accepting lower contributions today, while directing public subsidies to low-income individuals. We simulate the future evolution of the financial balance of Algeria’s retirement system under various contributable salary caps versus various scenarios of environmental evolution and potential parametric reform actions. The results demonstrated that a 40% cap, along with major parametric reforms and an ideal environment, would help achieve a cumulatively balanced system in the long run.
Robust Adaptive-Sliding-Mode Control for Teleoperation Systems with Time-Varying Delays and Uncertainties
Authors: Chang Yeong-Hwa , Yang Cheng-Yuan , Lin Hung-Wei
Published: June 2024

Master–slave teleoperation systems with haptic feedback enable human operators to interact with objects or perform tasks in remote environments. This paper presents a sliding-mode control scheme tailored for bilateral teleoperation systems operating in the presence of unknown uncertainties and time-varying delays. To address unknown but bounded uncertainties, adaptive laws are derived alongside controller design. Additionally, a linear matrix inequality is solved to determine the allowable bound of delays. Stability of the closed-loop system is ensured through Lyapunov–Krasovskii functional analysis. Two-degree-of-freedom mechanisms are self-built as haptic devices. Free-motion and force-perception scenarios are examined, with experimental results validating and comparing performances. The proposed adaptive-sliding-control method increases the position performance from 58.48% to 82.55% and the force performance from 83.48% to 99.77%. The proposed control scheme demonstrates enhanced position tracking and force perception in bilateral teleoperation systems.