bluuebunny/crossref_metadata_2025 huggingface dataset:
Daytime or Edge-of-Daytime Intra-Canopy Illumination Improves the Fruit Set of Bell Pepper at Passive Conditions in the Winter
Authors: Tiwari Vivekanand , Kamara Itzhak , Ratner Kira , Many Yair , Lukyanov Victor , Ziv Carmit , Gilad Ziva , Esquira Itzhak , Charuvi Dana
Published: February 2022

Optimal light conditions ensure the availability of sufficient photosynthetic assimilates for supporting the survival and growth of fruit organs in crops. One of the growing uses of light-emitting diodes (LEDs) in horticulture is intra-canopy illumination or LED-interlighting, providing supplemental light for intensively cultivated crops directly within their canopies. Originally developed and applied in environmentally controlled greenhouses in northern latitude countries, this technique is nowadays also being tested and studied in other regions of the world such as the Mediterranean region. In the present work, we applied intra-canopy illumination for bell pepper grown in passive high tunnels in the Jordan Valley using a commercial LED product providing cool-white light. The study included testing of daytime (‘LED-D’) and edge-of-daytime (‘LED-N’) illumination, as well as a detailed characterization of fruit set and fruit survival throughout the growth season. We found that both light regimes significantly improved the fruit set and survival during winter, with some benefit of LED-N illumination. Notably, we found that western-facing plants of illuminated sections had a higher contribution toward the increased winter fruit set and spring yield than that of illuminated eastern-facing plants. Greater plant height and fresh weight of western-facing plants of the illuminated sections support the yield results. The differences likely reflect higher photosynthetic assimilation of western-facing plants as compared to eastern-facing ones, due to the higher daily light integral and higher canopy temperature of the former. This study provides important implications for the use of intra-canopy lighting for crops grown at passive winter conditions and exemplifies the significance of geographical positioning, opening additional avenues of investigation for optimization of its use for improving fruit yield under variable conditions.
Virus-Induced Gene Silencing in Chrysanthemum seticuspe Using the Tomato Aspermy Virus Vector
Authors: Murai Hirotomo , Mochizuki Tomofumi
Published: February 2022

Chrysanthemum is one of the most economically important flowers globally due to its high ornamental value. In recent years, a large percentage of the Chrysanthemum seticuspe genome has been determined, making this species useful as a model chrysanthemum plant. To fully utilize the genome’s information, efficient and rapid gene functional analysis methods are needed. In this study, we optimized the tomato aspermy virus (TAV) vector for virus-induced gene silencing (VIGS) in C. seticuspe. Conventional plant virus inoculation methods, such as the mechanical inoculation of viral RNA transcripts and agroinoculation into leaves, did not achieve successful TAV infections in C. seticuspe, but vacuum infiltration into sprouts was successful without symptoms. The TAV vector harboring 100 nucleotides of the phytoene desaturase (PDS) gene caused photobleaching phenotypes and a reduction in CsPDS expression in C. seticuspe. To our knowledge, this is the first report of VIGS in chrysanthemums.
The Effect of Monoculture, Crop Rotation Combinations, and Continuous Bare Fallow on Soil CO2 Emissions, Earthworms, and Productivity of Winter Rye after a 50-Year Period
Authors: Bogužas Vaclovas , Skinulienė Lina , Butkevičienė Lina Marija , Steponavičienė Vaida , Petrauskas Ernestas , Maršalkienė Nijolė
Published: February 2022

One of the main goals of the 21st century’s developing society is to produce the necessary amount of food while protecting the environment. Globally, particularly in Lithuania and other northern regions with similar climatic and soil conditions, there is a lack of data on the long-term effects of crop rotation under the current conditions of intensive farming and climate change. It has long been recognized that monocultures cause soil degradation compared to crop rotation. Research hypothesis: the long-term implementation of crop rotation makes a positive influence on the soil environment. The aim of our investigation was to compare the effects of a 50-year-long application of different crop rotations and monocultures on soil CO2 emissions, earthworms, and productivity of winter rye. Long-term stationary field experiments were established in 1966 at Vytautas Magnus University Experimental Station (54°53′ N, 23°50′ E). The study was conducted using intensive field rotation with row crops, green manure crop rotations, three-course rotation, and rye monoculture. Pre-crop had the largest impact on soil CO2 emissions, and more intensive soil CO2 emissions occurred at the beginning of winter rye growing season. Rye appeared not to be demanding in terms of pre-crops. However, its productivity decreased when grown in monoculture, and the optimal mineral fertilization remained lower than with crop rotation, but productivity remained stable.
Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity
Authors: Kaashyap Mayank , Ford Rebecca , Mann Anita , Varshney Rajeev K. , Siddique Kadambot H. M. , Mantri Nitin
Published: February 2022

Salinity is increasingly becoming a significant problem for the most important yet intrinsically salt-sensitive grain legume chickpea. Chickpea is extremely sensitive to salinity during the reproductive phase. Therefore, it is essential to understand the molecular mechanisms by comparing the transcriptomic dynamics between the two contrasting genotypes in response to salt stress. Chickpea exhibits considerable genetic variation amongst improved cultivars, which show better yields in saline conditions but still need to be enhanced for sustainable crop production. Based on previous extensive multi-location physiological screening, two identified genotypes, JG11 (salt-tolerant) and ICCV2 (salt-sensitive), were subjected to salt stress to evaluate their phenological and transcriptional responses. RNA-Sequencing is a revolutionary tool that allows for comprehensive transcriptome profiling to identify genes and alleles associated with stress tolerance and sensitivity. After the first flowering, the whole flower from stress-tolerant and sensitive genotypes was collected. A total of ~300 million RNA-Seq reads were sequenced, resulting in 2022 differentially expressed genes (DEGs) in response to salt stress. Genes involved in flowering time such as FLOWERING LOCUS T (FT) and pollen development such as ABORTED MICROSPORES (AMS), rho-GTPase, and pollen-receptor kinase were significantly differentially regulated, suggesting their role in salt tolerance. In addition to this, we identify a suite of essential genes such as MYB proteins, MADS-box, and chloride ion channel genes, which are crucial regulators of transcriptional responses to salinity tolerance. The gene set enrichment analysis and functional annotation of these genes in flower development suggest that they can be potential candidates for chickpea crop improvement for salt tolerance.
Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review
Authors: Hussain Masooma Jawad , Abbas Yawar , Nazli Naushaba , Fatima Sara , Drouet Samantha , Hano Christophe , Abbasi Bilal Haider
Published: February 2022

Medicinal plants are an inevitable source of pharmaceutical drugs and most of the world population depends on these plants for health benefits. The increasing global demand for bioactive compounds from medicinal plants has posed a great threat to their existence due to overexploitation. Adventitious root and hairy root culture systems are an alternative approach to the conventional method for mass production of valuable compounds from medicinal plants owing to their rapid growth, biosynthetic and genetic stability. The main purpose of this review is to investigate the recent scientific research published worldwide on the application of adventitious and hairy root cultures to produce valuable compounds from medicinal plants. Furthermore, a comparison of adventitious root vs. hairy root cultures to produce valuable compounds has also been discussed. Various aspects such as medium composition, carbon source, pH, amount of macronutrients, optimization strategy, scale-up cultures, and use of biotic abiotic and nano-elicitors at various concentrations are the topic of discussion in this review. Several studies on adventitious and hairy root cultures of Polygonum multiflorum¸ Withania somnifera¸ Echinacea purpurea and Ajuga bracteosa have been discussed in detail which highlights the importance of elicitation strategies and bioreactor system, presenting commercial applications.
Transcriptome Analysis Revealed a Positive Role of Ethephon on Chlorophyll Metabolism of Zoysia japonica under Cold Stress
Authors: Zhang Jiahang , Zhang Zhiwei , Liu Wen , Li Lijing , Han Liebao , Xu Lixin , Zhao Yuhong
Published: February 2022

Zoysia japonica is a warm-season turfgrass with a good tolerance and minimal maintenance requirements. However, its use in Northern China is limited due to massive chlorophyll loss in early fall, which is the main factor affecting its distribution and utilization. Although ethephon treatment at specific concentrations has reportedly improved stress tolerance and extended the green period in turfgrass, the potential mechanisms underlying this effect are not clear. In this study, we evaluated and analyzed chlorophyll changes in the physiology and transcriptome of Z. japonica plants in response to cold stress (4 °C) with and without ethephon pretreatment. Based on the transcriptome and chlorophyll content analysis, ethephon pretreatment increased the leaf chlorophyll content under cold stress by affecting two processes: the stimulation of chlorophyll synthesis by upregulating ZjMgCH2 and ZjMgCH3 expression; and the suppression of chlorophyll degradation by downregulating ZjPAO, ZjRCCR, and ZjSGR expression. Furthermore, ethephon pretreatment increased the ratio of chlorophyll a to chlorophyll b in the leaves under cold stress, most likely by suppressing the conversion of chlorophyll a to chlorophyll b due to decreased chlorophyll b synthesis via downregulation of ZjCAO. Additionally, the inhibition of chlorophyll b synthesis may result in energy redistribution between photosystem II and photosystem I.
Comparison of Magnoliaceae Plastomes: Adding Neotropical Magnolia to the Discussion
Authors: Guzmán-Díaz Salvador , Núñez Fabián Augusto Aldaba , Veltjen Emily , Asselman Pieter , Larridon Isabel , Samain Marie-Stéphanie
Published: February 2022

Chloroplast genomes are considered to be highly conserved. Nevertheless, differences in their sequences are an important source of phylogenetically informative data. Chloroplast genomes are increasingly applied in evolutionary studies of angiosperms, including Magnoliaceae. Recent studies have focused on resolving the previously debated classification of the family using a phylogenomic approach and chloroplast genome data. However, most Neotropical clades and recently described species have not yet been included in molecular studies. We performed sequencing, assembly, and annotation of 15 chloroplast genomes from Neotropical Magnoliaceae species. We compared the newly assembled chloroplast genomes with 22 chloroplast genomes from across the family, including representatives from each genus and section. Family-wide, the chloroplast genomes presented a length of about 160 kb. The gene content in all species was constant, with 145 genes. The intergenic regions showed a higher level of nucleotide diversity than the coding regions. Differences were higher among genera than within genera. The phylogenetic analysis in Magnolia showed two main clades and corroborated that the current infrageneric classification does not represent natural groups. Although chloroplast genomes are highly conserved in Magnoliaceae, the high level of diversity of the intergenic regions still resulted in an important source of phylogenetically informative data, even for closely related taxa.
Flax and Sorghum: Multi-Element Contents and Nutritional Values within 210 Varieties and Potential Selection for Future Climates to Sustain Food Security
Authors: Hacisalihoglu Gokhan , Armstrong Paul R.
Published: February 2022

The Dietary Guidelines for Americans recommends giving priority to nutrient-dense foods while decreasing energy-dense foods. Although both flax (Linum usitatissimum) and sorghum (Sorghum bicolor) are rich in various essential minerals, their ionomes have yet to be investigated. Furthermore, previous studies have shown that elevated CO2 levels could reduce key nutrients in crops. In this study, we analyzed 102 flax and 108 sorghum varieties to investigate their ionomic variations (N, P, K, Ca, Mg, S, B, Zn, Mn, Fe, Cu, and Mo), elemental level interactions, and nutritional value. The results showed substantial genetic variations and elemental correlations in flax and sorghum. While a serving size of 28 g of flax delivers 37% daily value (DV) of Cu, 31% of Mn, 28% of Mg, and 19% of Zn, sorghum delivers 24% of Mn, 16% of Cu, 11% of Mg, and 10% of Zn of the recommended daily value (DV). We identified a set of promising flax and sorghum varieties with superior seed mineral composition that could complement breeding programs for improving the nutritional quality of flax and sorghum. Overall, we demonstrate additional minerals data and their corresponding health and food security benefits within flax and sorghum that could be considered by consumers and breeding programs to facilitate improving seed nutritional content and to help mitigate human malnutrition as well as the effects of rising CO2 stress.
Sustainable Packaging Material Based on PCL Nanofibers and Lavandula luisieri Essential Oil, to Preserve Museological Textiles
Authors: Ferreira Ester F. , Mouro Cláudia , Silva Lúcia , Gouveia Isabel C.
Published: February 2022

The connection with textiles is one of the oldest traditions in humanity, and in the historical scenario, textiles and clothing deal with material culture. Therefore, preservation is of the utmost importance to keep this important heritage. Packaging and protection of museological textiles is imperative due to the risks that these articles suffer, mainly concerning the attack of microorganisms that promote the acceleration of their degradation, and it is still necessary to create a proper packing material. In the present work we describe a bibliographic review about the museological scenario, focused on the packaging for preservation of textile articles, as well as the techniques usually used in preventive material conservation. Future perpsctives for the improvement in the conservation of museological textiles are also given. This research aims to produce a sustainable material based on polycaprolactone (PCL), with and without antimicrobial function by incorporating Lavandula luisieri essential oil (EO), in the form of a non-woven substrate for museological packaging. A comparison was made with the most frequently used materials, such as raw cotton and a non-woven polyester. The results demonstrated that both PCL and PCL + EO obtained a good characterization for museological application with good breaking strength and excellent whiteness index. In addition, PCL + EO showed a high bacterial reduction when compared with other protective materials frequently used in museums. Therefore, these findings emphasize the potential use of this material as an innovative protective antibacterial museological packaging solution, able to safeguard and preserve textile museum and clothing collections for longer and for future generations.
Surface-Immobilized Photoinitiators for Light Induced Polymerization and Coupling Reactions
Authors: Mueller Matthias , Bandl Christine , Kern Wolfgang
Published: February 2022

Straightforward and versatile surface modification, functionalization and coating have become a significant topic in material sciences. While physical modification suffers from severe drawbacks, such as insufficient stability, chemical induced grafting processes efficiently modify organic and inorganic materials and surfaces due to covalent linkage. These processes include the “grafting from” method, where polymer chains are directly grown from the surface in terms of a surface-initiated polymerization and the “grafting to” method where a preformed (macro)-molecule is introduced to a preliminary treated surface via a coupling reaction. Both methods require an initiating species that is immobilized at the surface and can be triggered either by heat or light, whereas light induced processes have recently received increasing interest. Therefore, a major challenge is the ongoing search for suitable anchor moieties that provide covalent linkage to the surface and include initiators for surface-initiated polymerization and coupling reactions, respectively. This review containing 205 references provides an overview on photoinitiators which are covalently coupled to different surfaces, and are utilized for subsequent photopolymerizations and photocoupling reactions. An emphasis is placed on the coupling strategies for different surfaces, including oxides, metals, and cellulosic materials, with a focus on surface coupled free radical photoinitiators (type I and type II). Furthermore, the concept of surface initiation mediated by photoiniferters (PIMP) is reviewed. Regarding controlled radical polymerization from surfaces, a large section of the paper reviews surface-tethered co-initiators, ATRP initiators, and RAFT agents. In combination with photoinitiators or photoredox catalysts, these compounds are employed for surface initiated photopolymerizations. Moreover, examples for coupled photoacids and photoacid generators are presented. Another large section of the article reviews photocoupling and photoclick techniques. Here, the focus is set on light sensitive groups, such as organic azides, tetrazoles and diazirines, which have proven useful in biochemistry, composite technology and many other fields.
Detection of Organosulfur and Organophosphorus Compounds Using a Hexafluorobutyl Acrylate-Coated Tapered Optical Fibers
Authors: Stasiewicz Karol A. , Jakubowska Iwona , Dudek Michał
Published: February 2022

This paper presents the results of a study on the possibility of detecting organosulfur and organophosphorus compounds by means of polymer-assisted optical fiber technology. The detection of the aforementioned compounds can be realized by fabricating a polymer-coated tapered optical fiber (TOF), where the polymer works as an absorber, which changes the light propagation conditions in the TOF. The TOFs were manufactured based on a standard single-mode fiber for telecommunication purposes and, as an absorbing polymer, hexafluorobutyl acrylate was used, which is sensitive to organosulfur and organophosphorus compounds. The spectral measurements were conducted in a wide optical range—500–1800 nm—covering the visible part of the spectrum as well as near infrared part in order to show the versatility of the proposed solution. Additionally, detailed absorption dynamics measurements were provided for a single wavelength of 1310 nm. The analyses were conducted for two concentrations of evaporating compounds, 10 µL and 100 µL, in a volume of 150 mL. Additionally, a temperature dependency analysis and tests with distilled water were carried out to eliminate the influence of external factors. The results presented in this article confirmed the possibility to provide low-cost sensors for dangerous and harmful chemical compounds using optical fiber technology and polymers as sensitive materials.
Feasibility Study of the Flatness of a Plastic Injection Molded Pallet by a Newly Proposed Sequential Valve Gate System
Authors: Tsai Hsi Hsun , Liao Yi Lin
Published: February 2022

The investigation of plastic pallet molding, assisted by a sequential valve gate system, has not yet been performed due to the limitations of the pallet scale. Furthermore, at present, the application of recycled plastics by chemical industries has become extremely popular around the world. This study aimed to determine pallet flatness experimentally and numerically using recycled polypropylene with a large-scale pallet. Short-shot testing on injection molding was performed to obtain short-shot samples for confirmation of the flow front during simulated filling. The real injected pallet profile, which was measured by an ATOS, was compared after confirmation to the numerical profile of the pallet. The pallet’s flatness was accurately compared to the real experimental and numerical results. By adjusting the temperature of the cooling channel within the cavity plate to 55 °C, the flatness of the pallet achieved by the newly proposed sequential valve gate-opening scheme was about 7 mm, which meets the height directional warpage standard determined by the pre-set sequential scheme. The numerical flatness is in line with existing flatness values for pallets. Furthermore, the proposed cooling temperature gives the highest yield in terms of pallet molding from the perspective of the stakeholders.
Recent Progress on Bio-Based Polyesters Derived from 2,5-Furandicarbonxylic Acid (FDCA)
Authors: Fei Xuan , Wang Jinggang , Zhang Xiaoqin , Jia Zhen , Jiang Yanhua , Liu Xiaoqing
Published: February 2022

The big challenge today is the upgrading of sustainable materials to replace miscellaneous ones from petroleum resources. Thus, a generic bio-based building block lays the foundation of the huge bio-market to green economy. 2,5-Furandicarboxylic acid (FDCA), a rigid diacid derived from lignocellulose or fructose, represents a great potential as a contender to terephthalic acid (TPA). Recently, studies on the synthesis, modification, and functionalization of bio-based polyesters based on FDCA have attracted widespread attention. To apply furanic polyesters on engineering plastics, packaging materials, electronics, etc., researchers have extended the properties of basic FDCA-based homo-polyesters by directional copolymerization and composite preparation. This review covers the synthesis and performance of polyesters and composites based on FDCA with emphasis bedded on the thermomechanical, crystallization, barrier properties, and biodegradability. Finally, a summary of what has been achieved and the issues waiting to be addressed of FDCA-based polyester materials are suggested.
New Synthesis Routes toward Improvement of Natural Filler/Synthetic Polymer Interfacial Crosslinking
Authors: Nassar Mahmoud M. A. , Tarboush Belal J. Abu , Alzebdeh Khalid I. , Al-Hinai Nasr , Pervez Tasneem
Published: February 2022

Among the critical issues dictating bio-composite performance is the interfacial bonding between the natural fibers and polymer matrix. In this regard, this article presents new synthesis routes comprising the treatment and functionalization of both date palm powder (DPP) filler and a polypropylene (PP) matrix to enhance filler–polymer adhesion in the newly developed bio-composites. Specifically, four bio-composite forms are considered: untreated DPP filled PP (DPP-UT/PP), treated DPP filled PP (DPP-T/PP), treated DPP filled functionalized PP using 2-isocyanatoethyl methacrylate (DPP-T/PP-g-IEM), and treated and functionalized DPP using 4-toluenesulfonyl chloride filled functionalized PP using 2-acrylamide ((DPP-T)-g-TsCl/PP-g-AcAm). The functional groups created on the surface of synthesized PP-g-IEM react with activated hydroxyl groups attached to the filler, resulting in chemical crosslinking between both components. Similarly, the reaction of TsCl with NH2 chemical groups residing on the mating surfaces of the filler and polymer generates an amide bond in the interface region. Fourier transform infrared spectroscopy (FTIR) is used to confirm the successful coupling between the filler and polypropylene matrix after applying the treatment and functionalization schemes. Owing to the introduced crosslinking, the DPP-T/PP-g-IEM bio-composite exhibits the best mechanical properties as compared to the neat polymer, unfunctionalized polymer-based bio-composite, and (DPP-T)-g-TsCl/PP-g-AcAm counterpart. The applied compatibilizers assist in reducing the water uptake of the manufactured bio-composites, increasing their durability.
Simultaneous Optimization of Extraction Yield, Phenolic Compounds and Antioxidant Activity of Moroccan Propolis Extracts: Improvement of Ultrasound-Assisted Technique Using Response Surface Methodology
Authors: Aboulghazi Abderrazak , Bakour Meryem , Fadil Mouhcine , Lyoussi Badiaa
Published: February 2022

Propolis has given rise to refreshing interest in recent years in the field of conventional medicine. Its extraction represents an important process that requires optimal conditions, which strongly affect the yield of extraction, total polyphenols, flavonoid content, and radical scavenging capacity markers. The objective of the present study was to optimize the ultrasound-assisted extraction conditions of Moroccan propolis. The studied responses were the extraction yield, total polyphenols, flavonoid contents (TPC, TFC), and antioxidant activity of the extract evaluated by DPPH-IC50 and FRAP-EC50 assays. The response surface methodology (RSM) and specifically the Box–Behnken design (BBD) were used, taking into account three variables: sonication time (min), solvent/propolis ratio (mL/g), and ethanol concentration (%). After the realization of experiments and data analysis, optimal response values were 15.39%, 192 mg GAE/g of propolis,45.15 mg QEq/g, 29.8 µg/mL, and 128.3 µmol Fe2+/g for extraction yield, TPC, TFC, DPPH-IC50, and FRAP-EC50, respectively. Besides, optimal ultrasound extraction conditions were 15 min for sonication time, 30 mL/g for solvent/propolis ratio, and 40% for ethanol concentration. All obtained experimental values were in good agreement with the predicted values, suggesting that using an experimental design in the ultrasound-assisted extraction process and optimization was prudently chosen.
Bulk Process for Enrichment of Capsinoids from Capsicum Fruit
Authors: Cantrell Charles L. , Jarret Robert L.
Published: February 2022

Various methods to synthesize capsinoids (the nonpungent analogs of capsaicinoids) from precursor molecules have been reported. Capsinoids are also naturally present, at typically low concentrations, in the fruit of many Capsicum species and genotypes. However, they are also present in the fruit of select genotypes at high concentrations. The fruit of high-capsiate genotypes represents a commercial source of these compounds. To date, no method has been published that efficiently extracts and purifies capsinoids from Capsicum fruit in a rapid and simple bulk process. This study evaluated the efficacy of various organic solvents for the extraction of capsinoids from dried Capsicum annuum fruit. Among the organic solvents evaluated, pentane appeared to provide a good combination of both recovery and purity. A subsequent liquid/liquid extraction step, utilizing pentane and acetonitrile, resulted in 26.3% (wt/wt) capsiate and 19.4% (wt/wt) dihydrocapsiate for a combined capsinoids yield of 45.7% (wt/wt). A third step, involving a rapid hp20ss chromatography column using a water/acetonitrile gradient, resulted in a combined capsinoids yield of 96.6% (wt/wt).
Effects of Biochar Application on Vegetation Growth, Cover, and Erosion Potential in Sloped Cultivated Soil Derived from Mudstone
Authors: Lee Ming-Hsi , Lin Huan-Hsuan , Jien Shih-Hao
Published: February 2022

Soil degradation is a crucial problem, particularly in tropical and subtropical areas. Prevention or reduction of soil erosion requires strategies based on thorough rapid vegetation cover (VC) and favorable soil quality in subtropical and tropical areas. This study applied wood biochar (WB) and rice husk biochar (RHB) in a mudstone soil, which is widely distributed in Southern Taiwan, to investigate the effects of biochar application on soil erosion and vegetation restoration. The standard erosion unit plots (22.13 m in length and 9% in slope gradient) were set up to determine the relationship among soil losses, VC, and natural rainfall characteristics with and without biochar application. The results indicated that biochar application increased the growth rate (identified by cover ratio) of Bahia grass (Paspalum notatum Flüggé) by 2–2.6 times within 40 days compared with control (without biochar application) and increased VC by 20% after 120 days of treatment. The biochar application could effectively reduce soil losses by 60% at least in the mudstone soil. A well-predicted regression function of soil loss with VC and rainfall kinetic energy was established (amount of soil lost = −0.435 × ln VC + 0.54 × RKE, r = 0.89, p < 0.01).
Special Issue: Isolation and Utilization of Essential Oils: As Antimicrobials and Boosters of Antimicrobial Drug Activity
Authors: Sieniawska Elwira , Trifan Adriana , Greige-Gerges Hélène
Published: February 2022

In the search for new antimicrobial lead compounds, interest in natural-product-based screening has enjoyed a renaissance, driven by the fact that plants present a unique pool of compounds [...]
The Oxygen Gradient in Hypoxic Conditions Enhances and Guides Dictyostelium discoideum Migration
Authors: Hirose Satomi , Rieu Jean-Paul , Cochet-Escartin Olivier , Anjard Christophe , Funamoto Kenichi
Published: February 2022

Spatiotemporal variations of oxygen concentration affect the cell behaviors that are involved in physiological and pathological events. In our previous study with Dictyostelium discoideum (Dd) as a model of cell motility, aggregations of Dd cells exhibited long-lasting and highly stable migration in a self-generated hypoxic environment, forming a ring shape that spread toward the outer higher oxygen region. However, it is still unclear what kinds of changes in the migratory properties are responsible for the observed phenomena. Here, we investigated the migration of Dd to clarify the oxygen-dependent characteristics of aerokinesis and aerotaxis. Migratory behaviors of Dd cells were analyzed under various oxygen concentration gradients and uniform oxygen conditions generated in microfluidic devices. Under hypoxic conditions below 2% O2, corresponding to less than 25 µM O2 in the culture medium, the migration of Dd cells was enhanced (aerokinesis) and the oxygen gradient guided the cells toward the oxygen-rich region (aerotaxis). The aerotaxis was attributed to the increase in the frequency of migration associated with the direction of higher O2, the acceleration of migration velocity, and the enhancement of migration straightness. Thus, aerokinesis and aerotaxis are dependent on both the oxygen level and possibly relative gradient and are essential mechanisms for the migration of Dd.
Combating Sex Trafficking: The Role of the Hotel—Moral and Ethical Questions
Authors: Jeng Chu-Chuan , Huang Edward , Meo Sarah , Shelley Louise
Published: February 2022

Legitimate companies are key facilitators of human trafficking. These corporate facilitators include not only websites providing advertisements for commercial sex services but also hotels and motels. Analysis of all active federal criminal sex trafficking cases in 2018 and 2019 reveals that in approximately 80% of these cases, victims were exploited at either hotels or motels. This paper studies the prevalence of the hospitality industry in the crime of sex trafficking and the failure of this industry to address this problem until recent civil suits were filed by victims against individual hotels and chains. Drawing on the civil cases filed in federal courts by victims of human trafficking between 2015 and 2021 along the East Coast of the United States, this paper assesses the characteristics of these hotels and the conditions in the hotels that facilitated sex trafficking. The paper then explores the moral and ethical problems posed by the facilitating role of hotel owners/operators in sex trafficking either through collusion or failure to act on and/or report evidence of individual abuse. Suggestions on how to address the problem are provided.
We Will Not Shut Up and Dribble: LeBron James and the Tripartite Human Being
Authors: Scholes Jeffrey
Published: February 2022

On 15 February 2018, Fox News host Laura Ingraham ended her broadcast by responding to sharply critical comments that basketball stars LeBron James and Kevin Durant made about President Trump on the issue of race. Ingraham demanded that they “shut up and dribble,” to which James responded by creating a Twitter hashtag, #wewillnotshutupanddribble that adorned t-shirts worn during his next pregame warmup. In this essay, I contend that religion and sport come together throughout the “shut up and dribble” episode by way of a tripartite Christian anthropology of body, soul, and spirit that Ingraham is unwittingly conveying and James is adopting in his own way. This anthropology, as laid out in I Thessalonians and elsewhere, not only enables the reduction of Black human beings down to their body by silencing their souls, but also provides a framework for an athlete like LeBron James to counter these attacks.
Dynamics in Complex Systems Amidst Crisis 2008+: Financial Regulatory and Supervisory Reflections
Authors: Łasak Piotr , Wyciślak Sławomir
Published: February 2022

Every financial crisis triggers some regulatory and supervisory changes related to the ensuing threats. These regulations usually address specific types of risks and reduce them but do not protect the entire system from another crisis. The aim of this study was to develop a conceptual framework of financial system resilience based on the theoretical approach of complex system theory and its explanation of these systems’ self-adaptation. Our analysis embraces the time since the 2008+ financial crisis in the United States. We argue that the digitalization of financial markets may contribute to the greater safety of the banking sector. We adopted blockchain technology for the pattern of self-modification mechanisms of the financial system. The main findings highlight that the blockchain technology incorporated into the system approach and applied to financial regulation and supervision can significantly improve the safety of the financial markets.
The Risks of Smart Cities and the Perspectives of Their Management Based on Corporate Social Responsibility in the Interests of Sustainable Development
Authors: Morozova Irina A. , Yatsechko Stanislav S.
Published: February 2022

Purpose: Bring to light the risks of smart cities and the perspectives of their management. It has been discovered that smart cities are created and developed under the impact of not only technological factors but also social factors. The connection between smart cities and quality of life is systemic (direct and reverse)—the quality of life also specifies the creation and development of smart cities. The impact of the COVID-19 pandemic on the development of smart cities is almost null (smart cities do not depend on the implementation of SDG 3). This paper’s originality lies in the description of a new angle of studying smart cities—from the position of risks, and in the determination of the current level of these risks and the dynamics of their change during systematisation and description of the wide international experience of creation and development of smart cities. This paper’s uniqueness lies in the development of a new approach to managing the creation and development of smart cities, which is based on corporate social responsibility, thus specifying and ensuring the involvement and important role of the subjects of entrepreneurship in this process. It is proved that the contribution of smart cities to the implementation of the SDGs is much wider and goes beyond the limits of SDG 9—it also extends to SDG 1 and SDGs 11–13.
Deep Learning for Vegetation Health Forecasting: A Case Study in Kenya
Authors: Lees Thomas , Tseng Gabriel , Atzberger Clement , Reece Steven , Dadson Simon
Published: February 2022

East Africa has experienced a number of devastating droughts in recent decades, including the 2010/2011 drought. The National Drought Management Authority in Kenya relies on real-time information from MODIS satellites to monitor and respond to emerging drought conditions in the arid and semi-arid lands of Kenya. Providing accurate and timely information on vegetation conditions and health—and its probable near-term future evolution—is essential for minimising the risk of drought conditions evolving into disasters as the country’s herders directly rely on the conditions of grasslands. Methods from the field of machine learning are increasingly being used in hydrology, meteorology, and climatology. One particular method that has shown promise for rainfall-runoff modelling is the Long Short Term Memory (LSTM) network. In this study, we seek to test two LSTM architectures for vegetation health forecasting. We find that these models provide sufficiently accurate forecasts to be useful for drought monitoring and forecasting purposes, showing competitive performances with lower resolution ensemble methods and improved performances over a shallow neural network and a persistence baseline.
Identifying Precarious Settlements and Urban Fabric Typologies Based on GEOBIA and Data Mining in Brazilian Amazon Cities
Authors: dos Santos Bruno Dias , de Pinho Carolina Moutinho Duque , Oliveira Gilberto Eidi Teramoto , Korting Thales Sehn , Escada Maria Isabel Sobral , Amaral Silvana
Published: February 2022

Although 70% of the Amazon population lives in urban areas, studies on the urban Amazon are scarce. Much of the urban Amazon population lives in precarious settlements. The distinctiveness and diversity of Amazonian precarious settlements are vast and must be identified to be considered in the development of appropriate public policies. Aiming at investigating precarious settlements in Amazon, this study is guided by the following questions: For the Brazilian Amazon region, is it possible to identify areas of precarious settlements by combining geoprocessing and remote sensing techniques? Are there different typologies of precarious settlements distinguishable by their spatial arrangements? Thus, we developed a methodology for identifying precarious settlements and subsequently classifying them into urban fabric typologies (UFT), choosing the cities of Altamira, Cametá, and Marabá as study sites. Our classification model utilized geographic objects-based image analysis (GEOBIA) and data mining of spectral data from WPM sensor images from the CBERS-4A satellite, jointly with texture metrics, context metrics, biophysical index, voluntary geographical information, and neighborhood relationships. With the C5.0 decision tree algorithm we carried out variable selection and classification of these geographic objects. Our estimated models show accuracy above 90% when applied to the study sites. Additionally, we described Amazonian UFT in six types to be identified. We concluded that Amazonian precarious settlements are morphologically diverse, with an urban fabric different from those commonly found in Brazilian metropolitan areas. Identifying and characterizing distinct precarious areas is vital for the planning and development of sustainable and effective public policies for the urban Amazon.
The Influence of Climate Change on Forest Fires in Yunnan Province, Southwest China Detected by GRACE Satellites
Authors: Cui Lilu , Luo Chuanjiang , Yao Chaolong , Zou Zhengbo , Wu Guiju , Li Qiong , Wang Xiaolong
Published: February 2022

Yunnan province in China has rich forest resources but high forest fire frequency. Therefore, a better understanding of the relationship between climate change and forest fires in this region is important for forest fire prevention. This study used the Gravity Recovery and Climate Experiment (GRACE) terrestrial water storage change (TWSC) data to analyze the influence of climate change on forest fires in the region during 2003–2016. To improve the accuracy and reliability of GRACE TWSC data, we used the generalized three-cornered hat (GTCH) and the least square method to fuse TWSC data from six GRACE solutions. The spatiotemporal variation of forest fires during 2003–2016 was investigated using burned area data. Then, the relationship between burned area and hydrological and climatic factors was analyzed. The results indicate that more than 90% of burned areas are located in northwestern and southern Yunnan (NW and S). On the seasonal scale, forest fires are mainly concentrated in January–April (dry season) and the burned area is negatively correlated with precipitation (correlation coefficient r = −0.83 (NW) and −0.51 (S)), relative humidity (r = −0.79 (NW) and −0.92 (S)), GRACE TWSC (r = −0.57 (NW) and −0.73 (S)) and evapotranspiration (r = −0.90 (NW) and −0.35 (S)). However, the burned area has no significant correlations with the above four factors on the interannual scale. The composite analysis suggests that the extreme climate affects precipitation, evapotranspiration and TWSC in this region, thereby changing water storage of the air in this region, leading to the formation of an environment prone to forest fires. Such conditions have led to an increase in the burned area in the above region. We also found that the difference between TWSC in high- and low-fire years is much greater than the precipitation in the same period. The above results show that GRACE satellites can detect the influence of climate change on forest fires in Yunnan province.
A Comparative Analysis of Unmanned Aircraft Systems in Low Altitude Photogrammetric Surveys
Authors: Mugnai Francesco , Tucci Grazia
Published: February 2022

Comparing photogrammetric performances of four user-grade unmanned aircraft systems (UAS) is the main aim of this paper. This study investigates what is the more suitable UAS for specific applications considering the required scale factor, such as for architectural, environmental and restoration purposes. Some photogrammetric surveys were conducted in a 5 ha area using a Phantom 4 Adv, Mavic 2 Pro, Mavic Air 2 and Mavic Mini 2. These unmanned aircrafts are commercial systems used mainly by private professionals. Some photogrammetric reconstructions were carried out by varying flight altitude and camera settings of the 4 UAS. Structure-from-motion (SfM) algorithms were applied to the images taken from the UASs. The surveys’ quality was analyzed by comparing the ground targets’ coordinates measured on the field with indirect georeferencing through global navigation satellite system (GNSS). Fifty targets were installed and arranged following a kind of regular grid. For each photogrammetric flight, the boundary conditions were maintained the same, as well as the flight trajectories and the ground control point distribution. Altimetric and planimetric residuals were reported and compared for each photogrammetric survey. Using a regular grid of ground targets, the result obtained from Phantom 4 is one order of magnitude better than the ones obtained from the other UASs. Mavic Mini 2 leads to an error average of about 5 cm. Remembering that the Mavic Mini 2 is an ultralight drone (it does not require a pilot’s license), it could significantly reduce costs compared to all the others.
Extension of an Open GEOBIA Framework for Spatially Explicit Forest Stratification with Sentinel-2
Authors: Brauchler Melanie , Stoffels Johannes , Nink Sascha
Published: February 2022

Spatially explicit information about forest cover is fundamental for operational forest management and forest monitoring. Although open-satellite-based earth observation data in a spatially high resolution (i.e., Sentinel-2, ≤10 m) can cover some information needs, spatially very high-resolution imagery (i.e., aerial imagery, ≤2 m) is needed to generate maps at a scale suitable for regional and local applications. In this study, we present the development, implementation, and evaluation of a Geographic Object-Based Image Analysis (GEOBIA) framework to stratify forests (needleleaved, broadleaved, non-forest) in Luxembourg. The framework is exclusively based on open data and free and open-source geospatial software. Although aerial imagery is used to derive image objects with a 0.05 ha minimum size, Sentinel-2 scenes of 2020 are the basis for random forest classifications in different single-date and multi-temporal feature setups. These setups are compared with each other and used to evaluate the framework against classifications based on features derived from aerial imagery. The highest overall accuracies (89.3%) have been achieved with classification on a Sentinel-2-based vegetation index time series (n = 8). Similar accuracies have been achieved with classification based on two (88.9%) or three (89.1%) Sentinel-2 scenes in the greening phase of broadleaved forests. A classification based on color infrared aerial imagery and derived texture measures only achieved an accuracy of 74.5%. The integration of the texture measures into the Sentinel-2-based classification did not improve its accuracy. Our results indicate that high resolution image objects can successfully be stratified based on lower spatial resolution Sentinel-2 single-date and multi-temporal features, and that those setups outperform classifications based on aerial imagery only. The conceptual framework of spatially high-resolution image objects enriched with features from lower resolution imagery facilitates the delivery of frequent and reliable updates due to higher spectral and temporal resolution. The framework additionally holds the potential to derive additional information layers (i.e., forest disturbance) as derivatives of the features attached to the image objects, thus providing up-to-date information on the state of observed forests.
UAV-Based Remote Sensing for Managing Alaskan Native Heritage Landscapes in the Yukon-Kuskokwim Delta
Authors: Lim Jonathan S. , Gleason Sean , Williams Meta , Linares Matás Gonzalo J. , Marsden Daniel , Jones Warren
Published: February 2022

The Yukon-Kuskokwim (Y-K) Delta is home to the Alaskan Native Yup’ik people who have inhabited this remote, subarctic tundra for over 1500 years. Today, their ancestral lifeways and cultural landscapes are at risk from severe climate change-related threats. In turn, we propose that remote sensing technologies, particularly with sensors mounted on Unmanned Aerial Vehicle (UAV) platforms, are uniquely suited for protecting Yup’ik landscape heritage. Based on collaborative, community-based fieldwork in Quinhagak, AK, we present evidence that cultural sites—ranging from historic fishing camps to pre-contact winter villages—exhibit predictably atypical vegetation patterns based on the local ecological biome. Furthermore, these vegetation patterns can be recorded and statistically quantified through the analysis of multispectral imagery obtained from UAV-mounted sensors with three different false color composite rasters and vegetation indices depending on biome type. Finally, we suggest how the Yupiit can combine these methodologies/workflows with local knowledge to monitor the broader heritage landscape in the face of climate change.