bluuebunny/crossref_metadata_2025 huggingface dataset:
Adverse drug events associated with linezolid administration: a real-world pharmacovigilance study from 2004 to 2023 using the FAERS database
Authors: Zou Fan , Cui Zhiwei , Lou Siyu , Ou Yingyong , Zhu Chengyu , Shu Chengjie , Chen Junyou , Zhao Ruizhen , Wu Zhu , Wang Li , Chen Zhenyong , Chen Huayu , Lan Yuanbo
Published: February 2024

Introduction: Linezolid is an oxazolidinone antibiotic that is active against drug-resistant Gram-positive bacteria and multidrug-resistant Mycobacterium tuberculosis. Real-world studies on the safety of linezolid in large populations are lacking. This study aimed to determine the adverse events associated with linezolid in real-world settings by analyzing data from the US Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS).

Methods: We retrospectively extracted reports on adverse drug events (ADEs) from the FAERS database from the first quarter of 2004 to that of 2023. By using disproportionality analysis including reporting odds ratio (ROR), proportional reporting ratio (PRR), Bayesian Confidence Propagation Neural Network (BCPNN), along with the multi-item gamma Poisson shrinker (MGPS), we evaluated whether there was a significant association between linezolid and ADE. The time to onset of ADE was further analyzed in the general population and within each age, weight, reporting population, and weight subgroups.

Results: A total of 11,176 reports of linezolid as the “primary suspected” drug and 263 significant adverse events of linezolid were identified, including some common adverse events such as thrombocytopenia (n = 1,139, ROR 21.98), anaemia (n = 704, ROR 7.39), and unexpected signals that were not listed on the drug label such as rhabdomyolysis (n = 90, ROR 4.33), and electrocardiogram QT prolonged (n = 73, ROR 4.07). Linezolid-induced adverse reactions involved 27 System Organ Class (SOC). Gender differences existed in ADE signals related to linezolid. The median onset time of all ADEs was 6 days, and most ADEs (n = 3,778) occurred within the first month of linezolid use but some may continue to occur even after a year of treatment (n = 46).

Conclusion: This study reports the time to onset of adverse effects in detail at the levels of SOC and specific preferred term (PT). The results of our study provide valuable insights for optimizing the use of linezolid and reducing potential side effects, expected to facilitate the safe use of linezolid in clinical settings.
Study of alloferon, a novel immunomodulatory antimicrobial peptide (AMP), and its analogues
Authors: Appiah Clara , Chen Shitian , Pori Afia Ibnat , Retyunskiy Vladimir , Tzeng Chimeng , Zhao Ye
Published: February 2024

Antimicrobial peptides (AMPs) are widely distributed throughout the biosphere and represent a class of conserved peptide molecules with intrinsic antimicrobial properties. Their broad-spectrum antimicrobial activity and low risk to induce resistance have led to increased interest in AMPs as potential alternatives to traditional antibiotics. Among the AMPs, alloferon has been addressed due to its immunomodulatory properties that augment both innate and adaptive immune responses against various pathogens. Alloferon and its analogues have demonstrated pharmaceutical potential through their ability to enhance Natural Killer (NK) cell cytotoxicity and stimulate interferon (IFN) synthesis in both mouse and human models. Additionally, they have shown promise in augmenting antiviral and antitumor activities in mice. In this article, we provide a comprehensive review of the biological effects of alloferon and its analogues, incorporating our own research findings as well. These insights may contribute to a deeper understanding of the therapeutic potential of these novel AMPs.
Modulating embryonic signaling pathways paves the way for regeneration in wound healing
Authors: Frech Sophie , Lichtenberger Beate M.
Published: February 2024

Epithelial tissues, including the skin, are highly proliferative tissues with the capability to constant renewal and regeneration, a feature that is essential for survival as the skin forms a protective barrier against external insults and water loss. In adult mammalian skin, every injury will lead to a scar. The scar tissue that is produced to seal the wound efficiently is usually rigid and lacks elasticity and the skin’s original resilience to external impacts, but also secondary appendages such as hair follicles and sebaceous glands. While it was long thought that hair follicles develop solely during embryogenesis, it is becoming increasingly clear that hair follicles can also regenerate within a wound. The ability of the skin to induce hair neogenesis following injury however declines with age. As fetal and neonatal skin have the remarkable capacity to heal without scarring, the recapitulation of a neonatal state has been a primary target of recent regenerative research. In this review we highlight how modulating dermal signaling or the abundance of specific fibroblast subsets could be utilized to induce de novo hair follicles within the wound bed, and thus to shift wound repair with a scar to scarless regeneration.
The loss of plant functional groups increased arthropod diversity in an alpine meadow on the Tibetan Plateau
Authors: Lu Ningna , Yang Hainian , Zhou Xianhui , Tan Yun , Cai Wei , Jiang Qin , Lu Ying , Chen Yangyang , He Haocheng , Wang Sheng
Published: February 2024

Plant species loss, driven by global changes and human activities, can have cascading effects on other trophic levels, such as arthropods, and alter the multitrophic structure of ecosystems. While the relationship between plant diversity and arthropod communities has been well-documented, few studies have explored the effects of species composition variation or plant functional groups. In this study, we conducted a long-term plant removal experiment to investigate the impact of plant functional group loss (specifically targeting tall grasses and sedges, as well as tall or short forbs) on arthropod diversity and their functional groups. Our findings revealed that the removal of plant functional groups resulted in increased arthropod richness, abundance and the exponential of Shannon entropy, contrary to the commonly observed positive correlation between plant diversity and consumer diversity. Furthermore, the removal of different plant groups had varying impacts on arthropod trophic levels. The removal of forbs had a more pronounced impact on herbivores compared to graminoids, but this impact did not consistently cascade to higher-trophic arthropods. Notably, the removal of short forbs had a more significant impact on predators, as evidenced by the increased richness, abundance, the exponential of Shannon entropy, inverse Simpson index and inverse Berger-Parker index of carnivores and abundance of omnivores, likely attributable to distinct underlying mechanisms. Our results highlight the importance of plant species identity in shaping arthropod communities in alpine grasslands. This study emphasizes the crucial role of high plant species diversity in controlling arthropods in natural grasslands, particularly in the context of plant diversity loss caused by global changes and human activities.
CRISPR/Cas9-mediated enhancement of semi-dwarf glutinous traits in elite Xiangdaowan rice (Oryza sativa L.): targeting SD1 and Wx genes for yield and quality improvement
Authors: Wang Quanxiu , Gao Haolin , Liu Ke , Wang Honglin , Zhang Fan , Wei Lanmeng , Lu Kaijing , Li Mengmeng , Shi Yiming , Zhao Jinhui , Zhou Wei , Peng Bo , Yuan Hongyu
Published: February 2024

In rice cultivation, the traits of semi-dwarfism and glutinous texture are pivotal for optimizing yield potential and grain quality, respectively. Xiangdaowan (XDW) rice, renowned for its exceptional aromatic properties, has faced challenges due to its tall stature and high amylose content, resulting in poor lodging resistance and suboptimal culinary attributes. To address these issues, we employed CRISPR/Cas9 technology to precisely edit the SD1 and Wx genes in XDW rice, leading to the development of stable genetically homozygous lines with desired semi-dwarf and glutinous characteristics. The sd1-wx mutant lines exhibited reduced gibberellin content, plant height, and amylose content, while maintaining hardly changed germination rate and other key agronomic traits. Importantly, our study demonstrated that exogenous GA3 application effectively promoted growth by compensating for the deficiency of endogenous gibberellin. Based on this, a semi-dwarf glutinous elite rice (Oryza sativa L.) Lines was developed without too much effect on most agronomic traits. Furthermore, a comparative transcriptome analysis unveiled that differentially expressed genes (DEGs) were primarily associated with the anchored component of the membrane, hydrogen peroxide catabolic process, peroxidase activity, terpene synthase activity, and apoplast. Additionally, terpene synthase genes involved in catalyzing the biosynthesis of diterpenoids to gibberellins were enriched and significantly down-regulated. This comprehensive study provides an efficient method for simultaneously enhancing rice plant height and quality, paving the way for the development of lodging-resistant and high-quality rice varieties.
Mitigation of salt stress in lettuce by a biostimulant that protects the root absorption zone and improves biochemical responses
Authors: Zuzunaga-Rosas Javier , Calone Roberta , Mircea Diana M. , Shakya Rashmi , Ibáñez-Asensio Sara , Boscaiu Monica , Fita Ana , Moreno-Ramón Héctor , Vicente Oscar
Published: February 2024

Horticultural crops constantly face abiotic stress factors such as salinity, which have intensified in recent years due to accelerated climate change, significantly affecting their yields and profitability. Under these conditions, it has become necessary to implement effective and sustainable solutions to guarantee agricultural productivity and food security. The influence of BALOX®, a biostimulant of plant origin, was tested on the responses to salinity of Lactuca sativa L. var. longifolia plants exposed to salt concentrations up to 150 mM NaCl, evaluating different biometric and biochemical properties after 25 days of treatment. Control plants were cultivated under the same conditions but without the biostimulant treatment. An in situ analysis of root characteristics using a non-destructive, real-time method was also performed. The salt stress treatments inhibited plant growth, reduced chlorophyll and carotenoid contents, and increased the concentrations of Na+ and Cl- in roots and leaves while reducing those of Ca2+. BALOX® application had a positive effect because it stimulated plant growth and the level of Ca2+ and photosynthetic pigments. In addition, it reduced the content of Na+ and Cl- in the presence and the absence of salt. The biostimulant also reduced the salt-induced accumulation of stress biomarkers, such as proline, malondialdehyde (MDA), and hydrogen peroxide (H2O2). Therefore, BALOX® appears to significantly reduce osmotic, ionic and oxidative stress levels in salt-treated plants. Furthermore, the analysis of the salt treatments’ and the biostimulant’s direct effects on roots indicated that BALOX®’s primary mechanism of action probably involves improving plant nutrition, even under severe salt stress conditions, by protecting and stimulating the root absorption zone.
Parent training for disruptive behavior symptoms in attention deficit hyperactivity disorder: a randomized clinical trial
Authors: Paiva Gabrielle Chequer de Castro , de Paula Jonas Jardim , Costa Danielle de Souza , Alvim-Soares Antônio , Santos Daniel Augusto Ferreira e , Jales Julia Silva , Romano-Silva Marco Aurélio , Miranda Débora Marques de
Published: February 2024

Background

Attention-Deficit/Hyperactivity Disorder (ADHD) affects 5% of children and 2.5% of adults worldwide. Comorbidities are frequent, and Oppositional Defiant Disorder (ODD) reaches 50%. Family environment is crucial for the severity of behaviors and for prognosis. In middle-income countries, access to treatment is challenging, with more untreated children than those under treatment. Face-to-face behavioral parent training (PT) is a well-established intervention to improve child behavior and parenting.

Method

A clinical trial was designed to compare PT-online and face-to-face effects to a waiting list group. Outcomes were the ADHD and ODD symptoms, parental stress and styles, and quality of life. Families were allocated into three groups: standard treatment (ST), ST + PT online, and ST + Face-to-Face PT. We used repeated measures ANOVA for pre × post treatment analysis corrected for multiple comparisons.

Results and discussion

Parent training was effective in reducing symptoms of ADHD (p = 0.030) and ODD (p = 0.026) irrespective of modality (p = 1.000). The combination of ST and PT was also associated with better quality of life in the physical domain for patients (p = 0.009) and their parents (p = 0.050). In addition to preliminary data, online intervention seems effective for parenting and improving social acceptance of children. The potential to reach many by an online strategy with a self-directed platform may imply effectiveness with a low cost for public health to support parents’ symptoms management.
Toxoplasma gondii infection in people with schizophrenia is related to higher hair glucocorticoid levels
Authors: Beaumont Emy , Brodeur Jacques , Thomas Frédéric , Dujon Antoine M. ,   and Consortium Signature, Lupien Sonia J.
Published: February 2024

Introduction

Toxoplasma gondii (TG) is a common protozoan parasite infecting approximately one third of the human population. Animal studies have shown that this parasite can manipulate its host behavior. Based on this, human studies have assessed if TG can be involved in mental health disorders associated with important behavioral modifications such as schizophrenia. However, results have been discrepant. Given that TG has a strong impact on fear and risk-taking processes in animal studies and that fear and risk-taking behaviors are associated with the human stress response, we tested whether glucocorticoid biomarkers (salivary and hair) differ in people with schizophrenia and controls as a function of TG status.

Methods

We measured TG antibodies in blood samples, as well as salivary and hair glucocorticoid levels in 226 people with schizophrenia (19.9% women, mean age = 39 years old) and 129 healthy individuals (controls) (45.7% women, mean age = 41 years old).

Results

The results showed that people with schizophrenia infected with TG presented significantly higher hair glucocorticoid concentrations than non-infected people with schizophrenia. This effect was not found in control participants. No effect was observed for salivary glucocorticoid levels. Additionally, there were no associations between TG infection and positive psychotic symptoms nor impulsivity.

Discussion

These results show that people with schizophrenia present high levels of hair glucocorticoid levels only when they are infected with TG. Further studies performed in populations suffering from other mental health disorders are needed to determine if this effect is specific to schizophrenia, or whether it is generalized across mental health disorders.
Development and validation of a risk score model for predicting autism based on pre- and perinatal factors
Authors: Ou Jianjun , Dong Huixi , Dai Si , Hou Yanting , Wang Ying , Lu Xiaozi , Xun Guanglei , Xia Kun , Zhao Jingping , Shen Yidong
Published: February 2024

Background

The use of pre- and perinatal risk factors as predictive factors may lower the age limit for reliable autism prediction. The objective of this study was to develop a clinical model based on these risk factors to predict autism.

Methods

A stepwise logistic regression analysis was conducted to explore the relationships between 28 candidate risk factors and autism risk among 615 Han Chinese children with autism and 615 unrelated typically developing children. The significant factors were subsequently used to create a clinical risk score model. A chi-square automatic interaction detector (CHAID) decision tree was used to validate the selected predictors included in the model. The predictive performance of the model was evaluated by an independent cohort.

Results

Five factors (pregnancy influenza-like illness, pregnancy stressors, maternal allergic/autoimmune disease, cesarean section, and hypoxia) were found to be significantly associated with autism risk. A receiver operating characteristic (ROC) curve indicated that the risk score model had good discrimination ability for autism, with an area under the curve (AUC) of 0.711 (95% CI=0.679-0.744); in the external validation cohort, the model showed slightly worse but overall similar predictive performance. Further subgroup analysis indicated that a higher risk score was associated with more behavioral problems. The risk score also exhibited robustness in a subgroup analysis of patients with mild autism.

Conclusion

This risk score model could lower the age limit for autism prediction with good discrimination performance, and it has unique advantages in clinical application.
Web-based guided self-help cognitive behavioral therapy–enhanced versus treatment as usual for binge-eating disorder: a randomized controlled trial protocol
Authors: van Beers Ella , Melisse Bernou , de Jonge Margo , Peen Jaap , van den Berg Elske , de Beurs Edwin
Published: February 2024

Binge-eating disorder (BED) is a psychiatric disorder characterized by recurrent episodes of eating a large amount of food in a discrete period of time while experiencing a loss of control. Cognitive behavioral therapy-enhanced (CBT-E) is a recommended treatment for binge-eating disorder and is typically offered through 20 sessions. Although binge-eating disorder is highly responsive to CBT-E, the cost of treating these patients is high. Therefore, it is crucial to evaluate the efficacy of low-intensity and low-cost treatments for binge-eating disorder that can be offered as a first line of treatment and be widely disseminated. The proposed noninferiority randomized controlled trial aims to determine the efficacy of web-based guided self-help CBT-E compared to treatment-as-usual CBT-E. Guided self-help will be based on a self-help program to stop binge eating, will be shorter in duration and lower intensity, and will require fewer therapist hours. Patients with binge-eating disorder (N = 180) will be randomly assigned to receive guided self-help or treatment-as-usual. Assessments will take place at baseline, mid-treatment, at the end of treatment, and at 20- and 40-weeks post-treatment. Treatment efficacy will be measured by examining the reduction in binge-eating days in the previous 28 days between baseline and the end of treatment between groups, with a noninferiority margin (Δ) of 1 binge-eating day. Secondary outcomes will include full remission, body shape dissatisfaction, therapeutic alliance, clinical impairment, health-related quality of life, attrition, and an economic evaluation to assess cost-effectiveness and cost-utility. The moderators examined will be baseline scores, demographic variables, and body mass index. It is expected that guided self-help is noninferior in efficacy compared to treatment-as-usual. The proposed study will be the first to directly compare the efficacy and economically evaluate a low-intensity and low-cost binge-eating disorder treatment compared to treatment-as-usual. If guided self-help is noninferior to treatment-as-usual in efficacy, it can be widely disseminated and used as a first line of treatment for patients with binge-eating disorder. The Dutch trial register number is R21.016. The study has been approved by the Medical Research Ethics Committees United on May 25th, 2021, case number NL76368.100.21.
Girls referred for amenorrhea: analysis of a patient series from a specialist center
Authors: Mörö Sara , Kosola Silja , Holopainen Elina
Published: February 2024

Objective

Among adolescents, amenorrhea is a common reason for medical consultation. Despite the variety of underlying etiologies, the prevalence of the causes is incompletely understood. This study aimed to assess the demographic and etiological factors among patients with amenorrhea treated in a single specialist unit of adolescent gynecology.

Design

Retrospective register study.

Methods

Medical records of 438 girls evaluated for primary or secondary amenorrhea in a single tertiary care center between 2015 and 2019 were retrospectively reviewed. In all, 423 patients—171 with primary amenorrhea and 252 with secondary amenorrhea—were included in the study. Data on underlying conditions, anthropometric variables, and selected hormonal markers were analyzed.

Results

Functional hypogonadotropic hypogonadism was the most frequent reason for primary (56%) and secondary (78%) amenorrhea. It was mostly explained by lifestyle-related functional hypothalamic amenorrhea caused by disordered eating, intense exercise, energy deficiency, psychological stress, and their combinations.

Conclusion

Menstrual pattern is a significant indicator of overall health and well-being among adolescent girls and young women. Functional reasons behind primary and secondary amenorrhea are important to recognize. Treatment often requires long-term lifestyle modifications. The frequency of functional causes also implies that most amenorrhea cases are preventable.
Salivary levels of inflammatory and anti-inflammatory biomarkers in periodontitis patients with and without acute myocardial infarction: implications for cardiovascular risk assessment
Authors: Varma Sudhir , Thomas Biju , Subrahmanyam K. , Duarte Kimberly , Alsaegh Mohammed A. , Gopinath Divya , Kuriadom Sam T. , Narayanan Jayaraj , Desai Vijay B. , Khair Al Moutassem B. , Afrashtehfar Kelvin I.
Published: February 2024

Background

Periodontitis is initiated by a dysbiotic activity and furthermore leads to a chronic inflammatory response. The presence of pro-inflammatory markers plays an important role in the inflammatory load. Macrophage inflammatory protein-1 alpha (MIP-1α) and C-reactive protein (CRP) are pro- inflammatory biomarkers that quantify clinical and subclinical inflammation in cardiac ischemia in cardiac inflammation and disease. Adiponectin is an anti-inflammatory marker associated with good health. The susceptibility of periodontitis patients to cardiovascular events needs to be evaluated.

Objective

This study aims to assess the levels of biomarkers in periodontitis patients with and without acute myocardial infarction (AMI) compared to controls.

Material and methods

Pro-inflammatory and anti-inflammatory analytes were examined by collecting unstimulated saliva from three groups (n = 20/each): healthy individuals, individuals with stage III periodontitis, and post-myocardial infarction patients with stage III periodontitis. The samples were collected within 48 h of AMI.

Results

Adiponectin levels were significantly lower in patients with periodontitis with and without AMI compared to controls, while CRP and MIP-1α were significantly higher in patients with periodontitis with and without AMI compared to controls. The highest titers for MIP-1α and CRP were detected among patients with periodontitis with and AMI.

Conclusion

Our study provides possible evidence of the association between periodontitis and salivary analytes that occur in tandem with cardiovascular disease. The lower levels of Adiponectin and higher levels of CRP and MIP-1α in patients with periodontitis indicate that this condition is a potential risk factor for cardiovascular disease. The findings emphasize the importance of early detection and intervention for periodontitis patients to prevent cardiovascular events.
Efficient regeneration of in vitro derived plants and genetic fidelity assessment of Phalaenopsis orchid
Authors: Sarmah Dipika , Mohapatra Priyadarshani P. , Seleiman Mahmoud F. , Mandal Tapas , Mandal Nirmal , Pramanik Kartik , Jena Chinmaya , Sow Sumit , Alhammad Bushra Ahmed , Ali Nawab , Ranjan Shivani , Wasonga Daniel O.
Published: February 2024

This study uses inflorescence stalk node as explants to establish an efficient and quick Phalaenopsis orchids cloning procedure for the most significant monopodial orchid in floriculture, without callus formation. The current study aimed to develop a rapid and easy regeneration process utilizing flower stalk nodes as explants, while also evaluating the clonal fidelity of the in vitro micropropagated plants through the analysis of RAPD markers. The tissue-cultured plantlets were grown on a solidified half-strength Murashig and Skoog (MS) base medium enriched with 15% coconut water (CW), 150 mg L−1 activated charcoal, and a mixture of 6-benzylaminopurine, BAP (cytokinins) and α-napthalene acetic acid, NAA and indole 3-butyric acid, IBA (auxins). After 14 weeks of growth, the early production of shoot bud was reported in ½ MS medium enriched with 2.5 mg L−1 BAP alone. Maximum shoot bud multiplication was observed in ½ MS fortified with BAP (2.5 mg L−1) + NAA (1.0 mg L−1), while the lowest was observed in 1.5 mg L−1 BAP + 0.5 mg L−1 IBA after 4 months of culturing. In this investigation, roots emerged simultaneously with shoot elongation from the axil, indicating the absence of a distinct rooting stage. The largest number of roots (3.25) was produced by BAP (2.5 mg L−1) + IBA (1.0 mg L−1) compared to NAA. Control, on the other hand, displayed no signs of root growth. Tissue cultured plantlets with well developed root systems while planted in a potting mixture of brick and charcoal (1: 1) resulted in a 70% survival rate during hardening. The clonal faithfulness of in vitro regenerated crop plantlets to the mother plant was demonstrated by the DNA extraction method with ten micropropagated plants’ young leaves as well as the mother plant using random amplification of a polymorphic DNA marker.
Effect of tea polyphenols on intestinal barrier and immune function in weaned lambs
Authors: Xu Yuewen , Yin Fuquan , Wang Jialin , Wu Pengxin , Qiu Xiaoyuan , He Xiaolin , Xiao Yimei , Gan Shangquan
Published: February 2024

Introduction

The purpose of this study was to explore the effects of tea polyphenols on growth performance, cytokine content, intestinal antioxidant status and intestinal barrier function of lambs, in order to provide reference for intestinal health of ruminants.

Methods

Thirty weaned lambs (average initial weight 9.32 ± 1.72 kg) were randomly divided into five groups with six lambs in each group. The control group did not add anything but the basic diet mainly composed of Pennisetum and Corn, and the other four groups added 2, 4, 6 g/kg tea polyphenols and 50 mg/kg chlortetracycline to the basic diet, respectively. The experiment lasted for 42 days.

Results

Dietary tea polyphenols improved the growth and stress response and reduced intestinal permeability of lambs (p > 0.05), while CTC did not affect the final lamb weight (p > 0.05). Both tea phenols and CTC significantly reduced inflammatory factors and enhanced the immune system (p > 0.05). Dietary tea polyphenols increased villus height, villus height/crypt depth, secretory immunoglobulin A (p > 0.05), and antioxidant enzymes, while decreasing MDA and apoptosis in the intestine (p > 0.05). However, compared with other groups, the content of T-AOC in jejunum did not change significantly (p > 0.05). Tea polyphenols also increased claudin-1 levels in the duodenum, jejunum, and ileum more than CTC (p > 0.05). CTC had a limited effect on the mRNA expression of Occludin and ZO-1, while tea polyphenols increased these in both the duodenum and ileum (p > 0.05).

Conclusion

This study demonstrated that tea polyphenols can effectively improve the intestinal barrier of weaned lambs, and that they have anti-inflammatory and antioxidant effects similar to those of antibiotics. Thus, tea polyphenols could be used to replace antibiotics in ensuring safety of livestock products and in achieving the sustainable development of modern animal husbandry.
Governance and Public Health Decision-Making During the COVID-19 Pandemic: A Scoping Review
Authors: Asthana Sumegha , Mukherjee Sanjana , Phelan Alexandra L. , Standley Claire J.
Published: February 2024

Objective: We provide an in-depth understanding of how governance and decision-making during the COVID-19 pandemic has been empirically characterized in the literature to identify gaps in research and highlight areas that require further inquiry.

Methods: We searched peer-reviewed publications using empirical data published between Jan 1, 2020 and Jan 31, 2022 in three electronic databases to examine the process of governance and decision-making during the COVID-19 pandemic. Two authors independently screened the records and 24 publications were extracted for the review.

Results: Governance is analyzed by its level at national, sub-national, community and by its aspects of process, determinants and performance. While different methodological approaches are used, governance is conceptualized in four ways 1) characteristics and elements, 2) leadership, 3) application of power and 4) models or arrangements of governance.

Conclusion: For future pandemic preparedness, there is a need for more empirical research using a unified conceptual approach to governance, which integrates decision-making processes and can guide governance structures and mechanisms across different countries and contexts. We call for more inclusivity in who performs the research on governance and where.
Coordinated Control for the Trajectory Tracking of Four-Wheel Independent Drive–Four-Wheel Independent Steering Electric Vehicles Based on the Extension Dynamic Stability Domain
Authors: Qiao Yiran , Chen Xinbo , Yin Dongxiao
Published: February 2024

In order to achieve multi-objective chassis coordination control for 4WID-4WIS (four-wheel independent drive–four-wheel independent steering) electric vehicles, this paper proposes a coordinated control strategy based on the extension dynamic stability domain. The strategy aims to improve trajectory tracking performance, handling stability, and economy. Firstly, expert PID and model predictive control (MPC) are used to achieve longitudinal speed tracking and lateral path tracking, respectively. Then, a sliding mode controller is designed to calculate the expected yaw moment based on the desired vehicle states. The extension theory is applied to construct the extension dynamic stability domain, taking into account the linear response characteristics of the vehicle. Different coordinated allocation strategies are devised within various extension domains, providing control targets for direct yaw moment control (DYC) and active rear steering (ARS). Additionally, a compound torque distribution strategy is formulated to optimize driving efficiency and tire adhesion rate, considering the vehicle’s economy and stability requirements. The optimal wheel torque is calculated based on this strategy. Simulation tests using the CarSim/Simulink co-simulation platform are conducted under slalom test and double-lane change to validate the control strategy. The test results demonstrate that the proposed control strategy not only achieves good trajectory tracking performance but also enhances handling stability and economy during driving.
Innovation’s Performance: A Transnational Analysis Based on the Global Innovation Index
Authors: Coutinho Evelina Maria Oliveira , Au-Yong-Oliveira Manuel
Published: February 2024

The pandemic marked the beginning of a succession of events on a global scale (not the least of which is a greater concern for the environment and for quality of life/distance work) with a major impact on the economy. Innovation plays a key role in meeting the challenges of the future, but despite investment in innovation, global economic growth has fallen short of the expected performance. The aim of this study is to identify the factors with the greatest impact on the performance of innovation ecosystems based on the performance of the innovation ecosystems of 64 countries assessed by the Global Innovation Index 2022. The methodology consists of multiple hierarchical linear regressions, in which the impact factors on innovation ecosystems, measured through indicators, are the independent variables and innovation performance, in knowledge and technology and in creativity, are the dependent variables in an iterative process, using STATA/MP 18.0 data analysis software. The results indicate that human capital and research (the basis of business and innovative products aimed at filling gaps in the market are people with a good higher education, which is also linked to local university rankings) and business sophistication (highly qualified work, leveraging strategic partnerships, and with knowledge absorption capacity) are the main pillars determining innovation performance at a global level. Education (an educated workforce is of growing importance in the knowledge era), R&D investment (including support from the state in the form of tax incentives for whoever invests in R&D), innovation partnerships (for a faster, more open innovation effort), ecological sustainability (a new reinforced priority after COVID-19) and knowledge absorption (to absorb one must first detain valuable knowledge in the area) are the variables with the greatest impact on innovation performance. The work provides guidance on which areas should be prioritized in the development of policies and strategies to accelerate innovation in countries. The study is limited by the time frame and reveals, by comparison with pre-pandemic studies, that the determinants of innovation can be dynamic, varying according to the countries and, consequently, the global context of the analysis.
Gliding Footprint Calculation Method for Aircraft with Thrust Failure Based on Six-Degree-of-Freedom Flight Envelope and Back-Propagation Artificial Neural Network
Authors: Chen Zhiwei , Ge Yuxue , Yuan Jie , Pei Yang
Published: February 2024

In hostile environments, engine damage is of particular concern since the engine is the only component to generate thrust that affects survivability. For an aircraft suffering thrust failure, the forced landing sites should be identified within the gliding footprint, which is the reachable region on the ground. This paper proposes two calculation methods to obtain the gliding footprint by finding a series of boundary points with maximum gliding distance around the aircraft. Method 1 models the thrust-failed aircraft with six-degree-of-freedom (6-DOF) flight dynamics and adopts a novel 6-DOF unpowered-flight envelope to characterize its maneuvering capabilities. Given the initial altitude when thrust failure occurs, Method 1 determines all feasible gliding distances around the aircraft based on the constructed 6-DOF flight envelope and selects the landing points of maximum gliding distances along different radial directions as the boundary points. Method 2 employs the Back-Propagation Artificial Neural Network (BP-ANN) to predict the boundary points. Using the well-trained BP-ANN, this method can estimate the maximum gliding distances with only the initial altitude and radial directions. Simulations are conducted to analyze these two methods. Compared with conventional methods using point-mass flight dynamics, Method 1 considers more flight constraints, and the gliding footprint area is reduced by 20.79%. These results are relatively conservative and can improve the safety threshold of forced landing sites. Method 2 can estimate the gliding footprints (encircled by the boundary points under the entire operational altitude and full radial direction) in real time, which reserves more response and action time for aircraft forced landing.
Multi-Physics Digital Model of an Aluminum 2219 Liquid Hydrogen Aircraft Tank
Authors: Tzoumakis George , Fotopoulos Konstantinos , Lampeas George
Published: February 2024

Future liquid hydrogen-powered aircraft requires the design and optimization of a large number of systems and subsystems, with cryogenic tanks being one of the largest and most critical. Considering previous space applications, these tanks are usually stiffened by internal members such as stringers, frames, and stiffeners resulting in a complex geometry that leads to an eventual reduction in weight. Cryogenic tanks experience a variety of mechanical and thermal loading conditions and are usually constructed out of several different materials. The complexity of the geometry and the loads highlights the necessity for a computational tool in order to conduct analysis. In this direction, the present work describes the development of a multi-physics finite element digital simulation, conducting heat transfer and structural analysis in a fully parametric manner in order to be able to support the investigation of different design concepts, materials, geometries, etc. The capabilities of the developed model are demonstrated by the design process of an independent-type aluminum 2219 cryogenic tank for commuter aircraft applications. The designed tank indicates a potential maximum take-off weight reduction of about 8% for the commuter category and demonstrates that aluminum alloys are serious candidate materials for future aircraft.
Investigating the Efficacy of Kidney-Protective Lactobacillus Mixture-Containing Pet Treats in Feline Chronic Kidney Disease and Its Possible Mechanism
Authors: Tsai Ching-Wen , Huang Hsiao-Wen , Lee Ya-Jane , Chen Ming-Ju
Published: February 2024

Microbiota-based strategies are a novel auxiliary therapeutic and preventative way of moderating chronic kidney disease (CKD). Lactobacillus mixture (Lm) was previously demonstrated to exert a renal-protective function in the CKD mice model. The efficacy of probiotics in pet foods is a relatively new area of study, and thus verifying the potential health benefits is necessary. This study evaluated the efficacy of Lm treats in feline CKD and elucidated the mechanisms underlying host-microbe interactions. CKD cats (2 and 3 stages) were administrated probiotic pet treats daily (10 g) for 8 weeks. The results demonstrated that during the eight weeks of Lm administration, creatinine was reduced or maintained in all cats with CKD. Similarly, gut-derived uremic toxin (GDUT), indoxyl sulfate (IS), were potential clinical significance in IS after Lm treatment (confidence intervals = 90%). The life quality of the cats also improved. Feline gut microbiome data, metabolic functional pathway, and renal function indicator analyses revealed the possible mechanisms involved in modulating CKD feline microbial composition. Further regulation of the microbial functions in amino acid metabolism after Lm administration contributed to downregulating deleterious GDUTs. The current study provides potential adjuvant therapeutic insights into probiotic pet foods or treats for pets with CKD.
Development of a Parturition Detection System for Korean Native Black Goats
Authors: Kim Heungsu , Kim Hyunse , Kim Woo H. , Min Wongi , Kim Geonwoo , Chang Honghee
Published: February 2024

Korean Native Black Goats deliver mainly during the cold season. However, in winter, there is a high risk of stunted growth and mortality for their newborns. Therefore, we conducted this study to develop a KNBG parturition detection system that detects and provides managers with early notification of the signs of parturition. The KNBG parturition detection system consists of triaxial accelerometers, gateways, a server, and parturition detection alarm terminals. Then, two different data, the labor and non-labor data, were acquired and a Decision Tree algorithm was used to classify them. After classifying the labor and non-labor states, the sum of the labor status data was multiplied by the activity count value to enhance the classification accuracy. Finally, the Labor Pain Index (LPI) was derived. Based on the LPI, the optimal processing time window was determined to be 10 min, and the threshold value for labor classification was determined to be 14 240.92. The parturition detection rate was 82.4%, with 14 out of 17 parturitions successfully detected, and the average parturition detection time was 90.6 min before the actual parturition time of the first kid. The KNBG parturition detection system is expected to reduce the risk of stunted growth and mortality due to hypothermia in KNBG kids by detecting parturition 90.6 min before the parturition of the first kid, with a success rate of 82.4%, enabling parturition nursing.
Diversity of Anaplasma phagocytophilum Strains from Roe Deer (Capreolus capreolus) and Red Deer (Cervus elaphus) in Poland
Authors: Myczka Anna W. , Steiner-Bogdaszewska Żaneta , Oloś Grzegorz , Bajer Anna , Laskowski Zdzisław
Published: February 2024

Background: The Gram-negative bacterium Anaplasma phagocytophilum is an intracellular pathogen and an etiological agent of human and animal anaplasmosis. Its natural reservoir comprises free-ranging ungulates, including roe deer (Capreolus capreolus) and red deer (Cervus elaphus). These two species of deer also constitute the largest group of game animals in Poland. The aim of the study was to genotype and perform a phylogenetic analysis of A. phagocytophilum strains from roe deer and red deer. Methods: Samples were subjected to PCR amplification, sequencing, and phylogenetic analysis of strain-specific genetic markers (groEL, ankA). Results: Five haplotypes of the groEL gene from A. phagocytophilum and seven haplotypes of ankA were obtained. The phylogenetic analysis classified the groEL into ecotypes I and II. Sequences of the ankA gene were classified into clusters I, II, and III. Conclusions: Strains of A. phagocytophilum from red deer were in the same ecotype and cluster as strains isolated from humans. Strains of A. phagocytophilum from roe deer represented ecotypes (I, II) and clusters (II, III) that were different from those isolated from red deer, and these strains did not show similarity to bacteria from humans. However, roe deer can harbor nonspecific strains of A. phagocytophilum more characteristic to red deer. It appears that the genetic variants from red deer can be pathogenic to humans, but the significance of the variants from roe deer requires more study.
Lack of Association between Antimicrobial Consumption and Antimicrobial Resistance in a HIV Preexposure Prophylaxis Population: A Cross-Sectional Study
Authors: Vanbaelen Thibaut , Laumen Jolein , Van Dijck Christophe , De Block Tessa , Manoharan-Basil Sheeba Santhini , Kenyon Chris
Published: February 2024

Background: In antibiotic naïve populations, there is a strong association between the use of an antimicrobial and resistance to this antimicrobial. Less evidence is available as to whether this relationship is weakened in populations highly exposed to antimicrobials. Individuals taking HIV preexposure prophylaxis (PrEP) have a high intake of antimicrobials. We previously found that there was no difference in the prevalence of pheno- and genotypic antimicrobial resistance between two groups of PrEP clients who had, and had not, taken antimicrobials in the prior 6 months. Both groups did, however, have a higher prevalence of resistance than a sample of the general population. Methods: In the current study, we used zero-inflated negative binomial regression models to evaluate if there was an individual level association between the consumption of antimicrobials and 1. the minimum inhibitory susceptibilities of oral Neisseria subflava and 2. the abundance of antimicrobial resistance genes in the oropharynges of these individuals. Results: We found no evidence of an association between the consumption of antimicrobials and the minimum inhibitory susceptibilities of oral Neisseria subflava or the abundance of antimicrobial resistance genes in these individuals. Conclusions: We conclude that in high-antimicrobial-consumption populations, the association between antimicrobial consumption and resistance may be attenuated. This conclusion would not apply to lower-consumption populations.
Expression of the Antimicrobial Peptide SE-33-A2P, a Modified Analog of Cathelicidin, and an Analysis of Its Properties
Authors: Gasanov Vagif , Vorotelyak Ekaterina , Vasiliev Andrey
Published: February 2024

In this study, we developed a method for the expression of the antimicrobial peptide SE-33-A2P in E. coli bacterial cells. The SE-33-A2P peptide consists of A2P and SE-33 peptides and is a retro analog of cathelicidin possessing antimicrobial activity against both Gram-positive and Gram-negative bacteria. Furthermore, the A2P peptide is a self-cleaving peptide. For an efficient expression of the SE-33-A2P peptide, a gene encoding several repetitive sequences of the SE-33 peptide separated by A2P sequences was created. The gene was cloned into a plasmid, with which E. coli cells were transformed. An induction of the product expression was carried out by IPTG after the cell culture gained high density. The inducible expression product, due to the properties of the A2P peptide, was cleaved in the cell into SE-33-A2P peptides. As the next step, the SE-33-A2P peptide was purified using filtration and chromatography. Its activity against both Gram-positive and Gram-negative bacteria, including antibiotic-resistant bacteria, was proved. The developed approach for obtaining a prokaryotic system for the expression of a highly active antimicrobial peptide expands the opportunities for producing antimicrobial peptides via industrial methods.
Microbial Precipitation of Calcium Carbonate for Crack Healing and Stabilization of Sandy Soils
Authors: Kim Yumi , Roh Yul
Published: February 2024

Microbially induced calcium carbonate (CaCO3) precipitation (MICP) can improve the shear strength of soil via biocementation while reducing its porosity and hydraulic conductivity. The purpose of this study was to evaluate the effect of the addition of bacterial metabolites and montmorillonite on the crack healing and biocementation of sandy soil during the MICP process. Cracks were generated by drying wet soil samples in Petri dishes, after which they were sprayed with one of four treatments: deionized water, a cementation solution, bacteria mixed with the cementation solution, and bacterial metabolites mixed with the cementation solution. After five cycles of this spray treatment, the surface crack ratio was observed to decrease by about 71% when living cells were used and by about 80% when microbial metabolites were added. However, the crack reduction ratio was relatively low when treated with water (28%) and the cementation solution alone (48%). To investigate the effect of adding a phyllosilicate to improve the strength of sandy soil, MICP was induced in sand mixed with 0–30% montmorillonite (MMT). As a result, the soil strength increased with higher levels of MMT, indicating that MMT contributed to soil stabilization as a colloid for CaCO3 precipitation and via adhesion between sand grains. Therefore, for the crack healing and stabilization of sandy soil, the addition of bacterial metabolites and montmorillonite may enhance the effectiveness of the MICP process.
Design of a Stacked Dual-Patch Antenna with 3D Printed Thick Quasi-Air Substrates and a Cavity Wall for Wideband Applications
Authors: Jang Doyoung , Lee Jun-Yong , Choo Hosung
Published: February 2024

In this paper, we propose a stacked dual-patch antenna with 3D printed thick quasi-air substrates and a cavity wall for wideband applications. To achieve the theoretical maximum bandwidth of the patch antenna, the quality factor of the system needs to be minimized. To achieve this, the area of the conductive radiator should be enlarged, while the permittivity of the substrate within the patch must be reduced close to 1. To realize a patch antenna with this maximum bandwidth, the stacked dual-patch configuration is employed to obtain an extended conductive radiator area. In addition, square-pipe resin frames manufactured using a 3D printing method are applied to the proposed antenna to implement a quasi-air substrate structure that has a low permittivity value close to 1. The proposed stacked dual-patch antenna with a quasi-air substrate has a broad bandwidth of 20.7%. The results demonstrate that by using the proposed antenna structure, broadband characteristics close to the fundamental bandwidth limit of the patch antenna can be achieved.