bluuebunny/crossref_metadata_2025 huggingface dataset:
Strategies towards Cost Reduction in the Manufacture of Printable Perovskite Solar Modules
Authors: Pourjafari Dena , Meroni Simone M. P. , Peralta Domínguez Diecenia , Escalante Renán , Baker Jenny , Saadi Monroy Alessary , Walters Adrian , Watson Trystan , Oskam Gerko
Published: January 2022

Among different perovskite solar cell architectures, the carbon-based perovskite solar cell (C-PSC) is a promising candidate for upscaling and commercialization related to low-cost components and simple manufacturing methods. For upscaling a PV technology, three parameters must be considered, corresponding to efficiency, stability, and cost. While the efficiency and lifetime of perovskite technology are the focus of many research groups, the cost parameter is less studied. This work aims to provide information on the manufacturing cost of C-PSC based on experimental data in order to give the readers a panoramic overview of parameters influencing a fabrication process. To analyze the commercialization viability of this technology, we estimated the cost of raw materials and the manufacturing process for sub-modules using two different methods: registration and scribing. The fabrication cost of a sub-module fabricated using the scribing method with 7.9% efficiency was approximately 44% less than that of a device with 6.8% efficiency prepared using registration. We demonstrated that this is due to both the design parameters and performance. In addition, we showed a 51% cost reduction for registration devices by appropriate choice of solar cell components, fabrication steps, and equipment based on the existing infrastructures for the manufacturing of large-scale devices.
Optimization of Operating Conditions of a Solid Oxide Fuel Cell System with Anode Off-Gas Recirculation Using the Model-Based Sensitivity Analysis
Authors: Choi Eun-Jung , Yu Sangseok , Lee Sang-Min
Published: January 2022

Designing a configuration of an efficient solid oxide fuel cell (SOFC) system and operating it under appropriate conditions are important for achieving a highly efficient SOFC system. In our previous research, the system layout of a SOFC system with anode off-gas recirculation was suggested, and the system performance was examined using a numerical model. In the present study, the system operating conditions were optimized based on the system configuration and numerical model developed in the previous paper. First, a parametric sensitivity analysis of the system performance was investigated to demonstrate the main operating parameters. Consequently, the fuel flow rate and recirculation ratio were selected. Then, the available operating conditions, which keep the system below the operating limits and satisfy the desired system performance (Ufuel > 0.7 and ηelec > 45%) were discovered. Finally, optimized operating conditions were suggested for three operating modes: optimized electrical efficiency, peak power, and heat generation. Depending on the situation, the demand for electricity and heat can be different, so different proper operating points are suggested for each mode. Additionally, using the developed model and the conducted process of this study, various optimized operating conditions can be derived for diverse cases.
A Thermohydraulic Performance of Internal Spiral Finned Tube Based on the Inner Tube Secondary Flow
Authors: Li Yicong , Qian Zuoqin , Wang Qiang
Published: January 2022

In this article, the BSL k-ω model was chosen as the turbulence model to simulate the heat transfer and flow characteristics of the proposed tubes inserted with internal spiral fins when the Re was set as 3000 to 17,000. The numerical results agreed well with the empirical formula. The average deviations of Nu and f between the simulation results and empirical formula results were 5.11% and 8.45%, respectively. By means of numerical simulation, the impact of three configurational parameters on the thermal performance was studied, namely the pitch P, the height H, and the number N of the internal spiral fins. The results showed that the Nu and f of the internal spiral finned tube were 1.77–3.74 and 3.04–10.62 times higher than those of smooth tube, respectively. PEC was also taken into account, ranging from 1.038 to 1.652. When the Re was set as 3000, the PEC achieved the peak value of 1.652 under the height H of the fins at 5 mm, the number N was 8, and the pitch P was 75 mm. However, with the increase of Re, the effect of pressure drop on the comprehensive performance in the tube was stronger than that of thermal enhancement. However, the PEC gradually decreased as the Re increased from 3000 to 17,000. In addition, the velocity and temperature fields were obtained to investigate the mechanisms of heat transfer enhancement.
Chamomile and Anise Cultivation in Olive Agroforestry Systems
Authors: Katsoulis Georgios I. , Kimbaris Athanassios C. , Anastasaki Eirini , Damalas Christos A. , Kyriazopoulos Apostolos P.
Published: January 2022

Agroforestry systems have been practiced for hundreds of years with multiple benefits both environmentally and economically in terms of productivity. Olive cultivation is widespread in the countries of the Mediterranean basin, including Greece. Agroforestry practices are common in olive groves, but little research has been conducted on the productivity of such systems, especially with medicinal–aromatic plants (MAPs) as understory crops. Natural populations of MAPs can be found in various ecosystems, while some of them are cultivated. The purpose of this research was to study the effects of fertilization and shading both on yield and chemical composition of essential oils derived from chamomile (Matricaria recutita L.) and anise (Pimpinella anisum L.), grown in olive silvoarable systems. Fertilization and shading increased the plant height of chamomile and delayed the flowering. In addition, fertilization increased the concentration of α-bisabolol oxide A and (Z)–spiroether, and reduced the α-bisabolone oxide A and hamazulen. Shade also reduced α-bisabolone oxide A and hamazulen but increased the α-bisabolol oxide B. In the case of anise, fertilization increased plant height, decreased the concentration of limonene, and increased the concentration of E-anethole. Shading reduced plant height. Intercropping of olive trees with chamomile and anise yielded essential oils rich in the substances defined by the commercial specifications.
The Role of Cations in Resorcinol–Formaldehyde Gel Textural Characteristics
Authors: Taylor Stewart J. , Yang Liu , Fletcher Ashleigh J.
Published: January 2022

The production of resorcinol–formaldehyde xerogels has yielded insight into the gelation processes underpinning their structures. In this work, the role of the cation species from the catalyst is probed by studying the simultaneous addition of sodium carbonate and calcium carbonate to a resorcinol–formaldehyde mixture. Twenty-eight xerogels were prepared by varying the solids content, catalyst concentration, and catalyst composition, and each was analysed for its textural properties, including the surface area and average pore diameter. The results indicate that the role of the cation is linked to the stabilisation of the clusters formed within the system, and that the Group II catalyst causes the salting out of the oligomers, resulting in fewer, larger clusters, hence, an increase in pore size and a broadening of the pore size distribution. The results provide insight into how these systems can be further controlled to create tailored porous materials for a range of applications.
Enhanced Antioxidant Activity of Fresh Fruits through Salicylic Acid/β-CD Hydroalcoholic Gels
Authors: Yang Zujin , Guan Youliang , Ji Hongbing
Published: January 2022

Oxidation is an important cause of fruit spoilage, and therefore improving the antioxidant capacity of fresh fruits is beneficial to their preservation. Herein, fresh-cut bananas were used as a type of fresh fruit and soaked in 75% hydroalcoholic gels containing salicylic acid (SA) or SA/β-CD inclusion complex (SA/β-CD). After treatment, they were placed in an atmosphere at 85% relative humidity at 20 °C for 12 days. A significant reduction in spoilage in bananas treated with the hydroalcoholic gels in the presence of SA/β-CD was observed, compared with those treated with gels in the presence or absence of SA. The free-radical-scavenging performances of SA and its complex were investigated using the DPPH (1,1-diphenyl-2-picryl-hydrazil) method. Based on the results, the significant increase in antioxidant activity was attributed to the fact that the inclusion complex could break the intramolecular hydrogen bonding of SA, thus efficiently eliminating ROS in the fruits. The formation of the inclusion complex was confirmed by experiments and theoretical calculations. Our findings indicate that treatment with SA/β-CD can provide an efficient method of maintaining postharvest quality and extending the shelf life of bananas.
A Simulated Shift Work Schedule Does Not Increase DNA Double-Strand Break Repair by NHEJ in the Drosophila Rr3 System
Authors: Bergerson Lydia , Fitzmaurice Caleb , Knudtson Tyler , McCormick Halle , Yu Alder M.
Published: January 2022

Long-term shift work is widely believed to increase the risk of certain cancers, but conflicting findings between studies render this association unclear. Evidence of interplay between the circadian clock, cell cycle regulation, and DNA damage detection machinery suggests the possibility that circadian rhythm disruption consequent to shift work could alter the DNA double-strand break (DSB) repair pathway usage to favor mutagenic non-homologous end-joining (NHEJ) repair. To test this hypothesis, we compared relative usage of NHEJ and single-strand annealing (SSA) repair of a complementary ended chromosomal double-stranded break using the Repair Reporter 3 (Rr3) system in Drosophila between flies reared on 12:12 and 8:8 (simulated shift work) light:dark schedules. Actimetric analysis showed that the 8:8 light:dark schedule effectively disrupted the rhythms in locomotor output. Inaccurate NHEJ repair was not a frequent outcome in this system overall, and no significant difference was seen in the usage of NHEJ or SSA repair between the control and simulated shift work schedules. We conclude that this circadian disruption regimen does not alter the usage of mutagenic NHEJ DSB repair in the Drosophila male pre-meiotic germline, in the context of the Rr3 system.
Benthic Foraminifera as Environmental Indicators in Mediterranean Marine Caves: A Review
Authors: Romano Elena , Bergamin Luisa , Parise Mario
Published: January 2022

Marine caves are characterized by wide environmental variability for the interaction between marine and continental processes. Their conditions may be defined as extreme for inhabiting organisms due to the enclosed morphology, lack of light, and scarcity of nutrients. Therefore, it is necessary to identify reliable ecological indicators for describing and assessing environmental conditions in these habitats even more than elsewhere. This review aims to provide the state of art related to the application of benthic foraminifera as proxies in the (paleo)ecological characterization of different habitats of marine caves. Special attention was addressed to a research project focused on Mediterranean marine caves with different characteristics, such as extent, morphology, freshwater influence, salinity, sediment type, oxygenation, and organic matter supply. This review aims to illustrate the reliability of foraminifera as an ecological and paleoecological indicator in these habitats. They respond to various environmental conditions with different assemblages corresponding to a very detailed habitat partitioning. Because marine caves may be considered natural laboratories for environmental variability, the results of these studies may be interpreted in the perspective of the global variability to understand the environmental drivers of future changes in marine systems.
Heterosis and Combining Ability for Fruit Yield, Sweetness, β-Carotene, Ascorbic Acid, Firmness and Fusarium Wilt Resistance in Muskmelon (Cucumis melo L.) Involving Genetic Male Sterile Lines
Authors: Kaur Simranpreet , Sharma Sat Pal , Sarao Navraj Kaur , Deol Jaideep Kaur , Gill Rupeet , Abd-Elsalam Kamel A. , Alghuthaymi Mousa A. , Hassan Mohamed M. , Chawla Neena
Published: January 2022

Ten genetically diverse inbred lines, including two genic male sterile lines, of muskmelon (Cucumis melo L.) were crossed in a half-diallel to generate 45 F1 hybrids. These hybrids, along with the parental lines and commercial check, were evaluated for their fruit yield, level of phytochemicals and Fusarium wilt resistance. Both additive and non-additive genetic variances were important in governing the expression of all of the traits; however, the additive gene action for the fruit weight (g), flesh thickness (cm), rind thickness (mm), firmness (lb inch−2), β-carotene content (mg/100 g), non-additive variance for fruit yield (t ha−1), fruit number, total soluble solids (TSS, °Brix), ascorbic acid (mg/100 g) and reaction to Fusarium wilt were comparatively more important. The parental line MM-625 was the best general combiner for fruit yield, rind thickness and β-carotene content (mg/100 g). The exotic line Riogold was the best combiner for flesh thickness and firmness. The netted inbred line MM-610 was the best general combiner for fruit weight, ascorbic acid and reaction to Fusarium wilt. The inbred lines KP4HM-15 and MM-916 were the best general combiners for the number of fruits per vine and TSS. The best cross-combinations for fruit yield ha−1 and TSS were MS-1×M-610 and Kajri×MM-904, respectively. The hybrids KP4HM-15×MM Sel-103 and KP4HM-15×MM-1831 recorded the highest standard heterosis for fruit yield and TSS. The landrace-derived inbred lines Kajri, MM Sel-103 and KP4HM-15 produced moderate-to-highly FW-resistant hybrids. Out of the 121 SSR markers applied, 70 exhibited parental polymorphism. The markers DM0561, CMAAAGN14, TJ147, CMMS35_3, CMAGN45 and DE1337 identified specific/unique alleles in certain parental genotypes. Thus, the findings of this study revealed that the novel inbred lines can effectively be combined to generate heterotic F1 hybrids for yield and other traits, such as rind and flesh thickness, TSS, β-carotene content and firmness. Furthermore, SSR markers can potentially be utilized to confirm the genetic diversity among the parental lines, and for the DNA fingerprinting of F1 hybrids.
The Cool Kids as a School-Based Universal Prevention and Early Intervention Program for Anxiety: Results of a Pilot Study
Authors: Scaini Simona , Rossi Federica , Rapee Ronald M. , Bonomi Francesca , Ruggiero Giovanni M. , Incerti Alessia
Published: January 2022

The efficacy of the Cool Kids program has been consistently demonstrated both within Australia and internationally, but limited data are available on the use of Cool Kids as a universal program. The purpose of the study is to evaluate Cool Kids as a universal program for preventing childhood anxiety in the school context. There were 73 Italian children (35 boys and 36 girls, ages 10–13 years) attending the last year of primary school and the first year of middle school who participated in an active intervention based on a school adaptation of the Cool Kids protocol. Results of t-test analyses highlighted a downward trend of anxiety symptoms, especially in total anxiety, somatic anxiety, generalized anxiety, separation anxiety, social anxiety and school phobia at post-treatment assessed by children. Even the score of depression symptoms, measured as a second outcome measure, decreased after the treatment. This study contributes to the evidence base for the Cool Kids program as a universal program for preventing childhood anxiety in the school context. Although these preliminary results show some promise, their replication in future research is necessary given current study limitations.
Detection of SARS-CoV-2 RNA in Bivalve Mollusks by Droplet Digital RT-PCR (dd RT-PCR)
Authors: Mancusi Andrea , Capuano Federico , Girardi Santa , Di Maro Orlandina , Suffredini Elisabetta , Di Concilio Denise , Vassallo Lucia , Cuomo Maria Concetta , Tafuro Maria , Signorelli Daniel , Pierri Andrea , Pizzolante Antonio , Cerino Pellegrino , La Rosa Giuseppina , Proroga Yolande Thérèse Rose , Pierri Biancamaria
Published: January 2022

Bivalve shellfish are readily contaminated by human pathogens present in waters impacted by municipal sewage, and the detection of SARS-CoV-2 in feces of infected patients and in wastewater has drawn attention to the possible presence of the virus in bivalves. The aim of this study was to collect data on SARS-CoV-2 prevalence in bivalve mollusks from harvesting areas of Campania region. A total of 179 samples were collected between September 2019 and April 2021 and were tested using droplet digital RT-PCR (dd RT-PCR) and real-time RT-PCR. Combining results obtained with different assays, SARS-CoV-2 presence was detected in 27/179 (15.1%) of samples. A median viral concentration of 1.1 × 102 and 1.4 × 102 g.c./g was obtained using either Orf1b nsp14 or RdRp/gene E, respectively. Positive results were unevenly distributed among harvesting areas and over time, positive samples being more frequent after January 2021. Partial sequencing of the spike region was achieved for five samples, one of which displaying mutations characteristic of the Alpha variant (lineage B.1.1.7). This study confirms that bivalve mollusks may bioaccumulate SARS-CoV-2 to detectable levels and that they may represent a valuable approach to track SARS-CoV-2 in water bodies and to monitor outbreak trends and viral diversity.
Participation in Physical Education Classes and Health-Related Behaviours among Adolescents from 67 Countries
Authors: Martins João , Marques Adilson , Gouveia Élvio Rúbio , Carvalho Francisco , Sarmento Hugo , Valeiro Miguel González
Published: January 2022

The present study sought to examine the associations between participation in physical education (PE) classes and a range of health-related behaviours among adolescents. Secondary analysis of self-reported data from the Global Student Health Survey, collected between 2010 and 2017 from 222,121 adolescents (N = 117,914 girls; 49.0%; aged 13–17 years) from 67 countries and five world regions, was carried out. Participation in PE classes (0, 1–2, ≥3 days/week) was the independent variable. Physical activity (PA); sedentary behaviour (SB); active travel to school; fruit, vegetables, and alcohol consumption; and smoking; as well as adopting ≥5 of these healthy behaviours; were the dependent variables. Complex samples logistic regressions were performed to explore the associations between participation in PE classes and health-related behaviours. The results revealed that 18.2% of adolescents did not take part in PE classes. A total of 56.7% and 25.1% of adolescents reported participating in PE classes on 1–2 and ≥3 days/week, respectively. Only 26.8% of adolescents adopted ≥5 healthy behaviours. Participation in PE classes was positively associated with PA, active travel, fruit consumption, and vegetable consumption (only for ≥3 days/week), but was negatively associated with meeting SB recommendations, and with not smoking (only for girls and ≥3 days/week). Overall, PE participation was positively associated with adopting ≥5 healthy behaviours, with favourable results found for those who attended more PE classes. The findings revealed a positive association between participation in PE classes and a range of health-related behaviours among adolescents. This suggests that, worldwide, quality PE should be delivered at least 3 days per week up to daily to promote healthy lifestyles among adolescents.
Project COALESCE—An Example of Academic Institutions as Conveners of Community-Clinic Partnerships to Improve Cancer Screening Access
Authors: Tossas Katherine Y. , Reitzel Savannah , Schifano Katelyn , Garrett Charlotte , Hurt Kathy , Rosado Michelle , Winn Robert A. , Thomson Maria D.
Published: January 2022

In Virginia, 56% of colorectal cancers (CRC) are diagnosed late, making it one of three enduring CRC mortality hotspots in the US. Cervical cancer (CCa) exhibits a similar pattern, with 48% late-stage diagnosis. Mortality for these cancers is worse for non-Latinx/e(nL)-Black people relative to nL-White people in Virginia, but preventable with equitable screening access and timely diagnostic follow-up. However, structural barriers, such as fractured referral systems and extended time between medical visits, remain. Because Federally Qualified Health Centers (FQHCs) care for a large proportion of racial and ethnic minorities, and underserved communities, regardless of ability to pay, they are ideal partners to tackle structural barriers to cancer screenings. We piloted a quality improvement initiative at five FQHCs in southcentral Virginia to identify and address structural, race-related barriers to CRC, as well as CCa screening and diagnostic follow-up using evidence-based approaches. Uniquely, FQHCs were paired with local community organizations in a didactic partnership, to elevate the community’s voice while together, increase support, acceptance, uptake, and intervention sustainability. We report on project development, and share preliminary data within the context of project goals, namely, to increase cancer screenings by 5–10%, improve knowledge and diagnostic follow-up processes, and build longitudinal partnerships.
Kinesiological Treatment of Early Spine Osteoarthritis in a Motorcyclist
Authors: Roggio Federico , Trovato Bruno , Ledda Caterina , Rapisarda Venerando , Musumeci Giuseppe
Published: January 2022

This case report speculates that the prolonged vibrations from enduro off-road sports are deleterious to the spine. The results of this case report may also aid sports physicians in better understanding this complex and relatively unknown phenomenon. No published data are present in the current literature that demonstrate the correlation between early spine osteoarthritis from enduro motorcycle overuse and the long-term management effects of a non-invasive kinesiological approach to reduce pain and inflammation and improve spine mobility and muscle strength.
Do Patients with Prostate Cancer Benefit from Exercise Interventions? A Systematic Review and Meta-Analysis
Authors: Andersen Martin Færch , Midtgaard Julie , Bjerre Eik Dybboe
Published: January 2022

Men diagnosed and treated for prostate cancer experience severe adverse effects on quality of life (QoL) and metabolic health, some of which may be preventable or reversible with exercise, the benefits of which healthcare providers and patients increasingly acknowledge, though existing evidence on its effects varies in significance and magnitude. We aimed to review the effect of exercise on QoL and metabolic health in a broad prostate cancer population. A systematic search was conducted in nine databases and eligible trials were included in the meta-analytic procedure. All outcomes were stratified into aerobic exercise, resistance exercise, and a combination of both. The review identified 33 randomised controlled trials (2567 participants) eligible for inclusion. Exercise had a borderline small positive effect on cancer-specific QoL (standardised mean difference (SMD) = 0.10, 95% confidence interval (CI) −0.01–0.22), and a moderate to large effect on cardiovascular fitness (SMD = 0.46, 95% CI 0.34–0.59) with aerobic exercise being the superior modality (SMD = 0.60, 95% CI 0.29–0.90). A positive significant effect was seen in lower body strength, whole-body fat mass, general mental health, and blood pressure. No significant effect was seen in fatigue, lean body mass, and general physical health. We thereby conclude that exercise is effective in improving metabolic health in men diagnosed with prostate cancer, with aerobic exercise as the superior modality. The effect of exercise on QoL was small and not mediated by choice of exercise modality.
Global Self-Esteem and Stress Intensity in a Group of Polish Nurses—A Mediatory Role of a Sense of Coherence
Authors: Kupcewicz Ewa
Published: January 2022

(1) Owing to their resistance resources, nurses can reduce the effects of stress, increase their commitment to work and improve their functioning in the face of challenges in the workplace. The aim of this study was to determine the mediatory role of a general sense of coherence and a sense of comprehensibility, manageability and meaningfulness correlated with global self-esteem and the perceived stress intensity in a group of Polish nurses aged 45–55 years. (2) The research using the diagnostic survey method was conducted on a group of 176 nurses (M = 49.1; SD = 3.1) working in seven hospitals located in Olsztyn (Poland). The following were used for data collection: Perceived Stress Scale - PSS-10, Rosenberg’s Self-Esteem Scale and Antonovsky’s Sense of Coherence (SOC-29) Questionnaire. (3) According to 21.02% of the nurses, their stress level at the workplace was low, 44.89% reported it was medium and 34.09% reported it was high. The self-esteem of nearly half of the nurses included in the study (48.30%) was at a medium level, 31.82% felt it was high and 19.89% felt it was low. The mediation analysis showed that a general sense of coherence and a sense of comprehensibility, manageability and meaningfulness have a mediator status in a correlation between global self-esteem and stress intensity. However, their mediatory role is partial. It is desirable for safe work environment promotion programmes to reinforce nurses’ personal resources, which can be helpful in coping with stressors.
Relationships between Occupational Stress, Change in Work Environment during the COVID-19 Pandemic, and Depressive and Anxiety Symptoms among Non-Healthcare Workers in Japan: A Cross-Sectional Study
Authors: Deguchi Yasuhiko , Iwasaki Shinichi , Niki Akihiro , Kadowaki Aya , Hirota Tomoyuki , Shirahama Yoshiki , Nakamichi Yoko , Okawa Yutaro , Uesaka Yuki , Inoue Koki
Published: January 2022

This study aims to clarify the effect of occupational stress and changes in the work environment on non-healthcare workers’ (HCWs) mental health during the third wave of the COVID-19 pandemic in Japan. A web-based, cross-sectional survey was conducted from 16 to 17 December 2020. Data from 807 non-HCWs were included. We evaluated occupational stress using the Generic Job Stress Questionnaire (GJSQ). Depressive and anxiety symptoms were assessed using the Japanese version of the Patient Health Questionnaire-9 and the Generalized Anxiety Disorder 7-item scale, respectively. We collected demographic variables, work-related variables, and the variables associated with COVID-19. The adjusted odds ratios for depressive and anxiety groups were estimated using multivariate logistic regression analyses, adjusted for all the demographic variables, work-related variables, COVID-19-related variables, and the six subdivided GJSQ subscales. The results confirm a relationship between variance in workload, job future ambiguity, social support from coworkers, having contact with COVID-19 patients, and depressive and anxiety symptoms. Paying attention to job future ambiguity, the variance in workload at the workplace and individual perspectives, promoting contact and support among coworkers using online communication tools, and reducing contact with COVID-19 patients, will be useful for decreasing the depressive and anxiety symptoms among non-HCWs.
Emerging Zoonotic Infections, Social Processes and Their Measurement and Enhanced Surveillance to Improve Zoonotic Epidemic Responses: A “Big Events” Perspective
Authors: Friedman Samuel R. , Jordan Ashly E. , Perlman David C. , Nikolopoulos Georgios K. , Mateu-Gelabert Pedro
Published: January 2022

Zoonotic epidemics and pandemics have become frequent. From HIV/AIDS through COVID-19, they demonstrate that pandemics are social processes as well as health occurrences. The roots of these pandemics lie in changes in the socioeconomic interface between humanity and non-human host species that facilitate interspecies transmission. The degree to which zoonoses spread has been increased by the greater speed and extent of modern transportation and trade. Pre-existing sociopolitical and economic structures and conflicts in societies also affect pathogen propagation. As an epidemic develops, it can itself become a social and political factor, and change and interact with pre-existing sociobehavioral norms and institutional structures. This paper uses a “Big Events” approach to frame these processes. Based on this framework, we discuss how social readiness surveys implemented both before and during an outbreak might help public health predict how overall systems might react to an epidemic and/or to disease control measures, and thus might inform interventions to mitigate potential adverse outcomes or possibly preventing outbreaks from developing into epidemics. We conclude by considering what “pathways measures”, in addition to those we and others have already developed, might usefully be developed and validated to assist outbreak and epidemic disease responses.
The Adverse Environmental Impact Factors Analysis on Fly-In-Fly-Out Personnel at Industrial Enterprises
Authors: Korneeva Yana
Published: January 2022

(1) Background: the research purpose is to identify and describe the general and different factors of adverse environmental impact on FIFO personnel at industrial enterprises at different levels of differential analysis of professional activity. (2) Methods: The research involved 359 employees of industrial enterprises with FIFO work organization. The study was carried out using a questionnaire, including a subjective assessment of the discomfort of three groups of negative environment impact factors to the FIFO personnel: climatic-geographical, industrial and social. (3) Results: The relationship between the increase in the degree of discomfort of production factors due to the in-fluence of climatic, geographical and social conditions has been established. With a various location of objects, the greatest discomfort is felt from the action of climatic and production factors; with varying degrees of group isolation and the shift period duration—all three groups, with the greatest influence of domestic and social; in various industries and enterprises—all three groups. (4) Conclusions: The differential analysis of the professional activities of FIFO personnel of industrial enterprises should be carried out at the following levels: the location of an industrial facility, the degree of group isolation, the duration of the shift period, the industry, the type of enterprise and the professional group.
A Fast and Accurate Spatial Target Snapping Method for 3D Scene Modeling and Mapping in Mobile Augmented Reality
Authors: Huang Kejia , Wang Chenliang , Liu Runying , Chen Guoxiong
Published: January 2022

High-performance spatial target snapping is an essential function in 3D scene modeling and mapping that is widely used in mobile augmented reality (MAR). Spatial data snapping in a MAR system must be quick and accurate, while real-time human–computer interaction and drawing smoothness must also be ensured. In this paper, we analyze the advantages and disadvantages of several spatial data snapping algorithms, such as the 2D computational geometry method and the absolute distance calculation method. To address the issues that existing algorithms do not adequately support 3D data snapping and real-time snapping of high data volumes, we present a new adaptive dynamic snapping algorithm based on the spatial and graphical characteristics of augmented reality (AR) data snapping. Finally, the algorithm is experimented with by an AR modeling system, including the evaluation of snapping efficiency and snapping accuracy. Through the experimental comparison, we found that the algorithm proposed in this paper is substantially improved in terms of shortening the snapping time, enhancing the snapping stability, and improving the snapping accuracy of vector points, lines, faces, bodies, etc. The snapping efficiency of the algorithm proposed in this paper is 1.6 times higher than that of the traditional algorithm on average, while the data acquisition accuracy based on the algorithm in this paper is more than 6 times higher than that of the traditional algorithm on average under the same conditions, and its data accuracy is improved from the decimeter level to the centimeter level.
Analyzing the Behaviors of OpenStreetMap Volunteers in Mapping Building Polygons Using a Machine Learning Approach
Authors: Hacar Müslüm
Published: January 2022

Mapping as an action in volunteered geographic information is complex in light of the human diversity within the volunteer community. There is no integrated solution that models and fixes all data heterogeneity. Instead, researchers are attempting to assess and understand crowdsourced data. Approaches based on statistics are helpful to comprehend trends in crowd-drawing behaviors. This study examines trends in contributors’ first decisions when drawing OpenStreetMap (OSM) buildings. The proposed approach evaluates how important the properties of a point are in determining the first point of building drawings. It classifies the adjacency types of the buildings using a random forest classifier for the properties and aids in inferring drawing trends from the relative impact of each property. To test the approach, detached and attached building groups in Istanbul and Izmir, Turkey, were used. The result had an 83% F-score. In summary, the volunteers tended to choose as first points those further away from the street and building centroid and provided lower point density in the detached buildings than the attached ones. This means that OSM volunteers paid more attention to open spaces when drawing the first points of the detached buildings in the study areas. The study reveals common drawing trends in building-mapping actions.
Photodegradation of Lipofuscin in Suspension and in ARPE-19 Cells and the Similarity of Fluorescence of the Photodegradation Product with Oxidized Docosahexaenoate
Authors: Różanowska Małgorzata B. , Różanowski Bartosz
Published: January 2022

Retinal lipofuscin accumulates with age in the retinal pigment epithelium (RPE), where its fluorescence properties are used to assess retinal health. It was observed that there is a decrease in lipofuscin fluorescence above the age of 75 years and in the early stages of age-related macular degeneration (AMD). The purpose of this study was to investigate the response of lipofuscin isolated from human RPE and lipofuscin-laden cells to visible light, and to determine whether an abundant component of lipofuscin, docosahexaenoate (DHA), can contribute to lipofuscin fluorescence upon oxidation. Exposure of lipofuscin to visible light leads to a decrease in its long-wavelength fluorescence at about 610 nm, with a concomitant increase in the short-wavelength fluorescence. The emission spectrum of photodegraded lipofuscin exhibits similarity with that of oxidized DHA. Exposure of lipofuscin-laden cells to light leads to a loss of lipofuscin granules from cells, while retaining cell viability. The spectral changes in fluorescence in lipofuscin-laden cells resemble those seen during photodegradation of isolated lipofuscin. Our results demonstrate that fluorescence emission spectra, together with quantitation of the intensity of long-wavelength fluorescence, can serve as a marker useful for lipofuscin quantification and for monitoring its oxidation, and hence useful for screening the retina for increased oxidative damage and early AMD-related changes.
Oncogenic Role of Exosomal Circular and Long Noncoding RNAs in Gastrointestinal Cancers
Authors: Yun Ba Da , Choi Ye Ji , Son Seung Wan , Cipolla Gabriel Adelman , Berti Fernanda Costa Brandão , Malheiros Danielle , Oh Tae-Jin , Kuh Hyo Jeong , Choi Soo Young , Park Jong Kook
Published: January 2022

Circular RNAs (circRNAs) and long noncoding RNAs (lncRNAs) are differentially expressed in gastrointestinal cancers. These noncoding RNAs (ncRNAs) regulate a variety of cellular activities by physically interacting with microRNAs and proteins and altering their activity. It has also been suggested that exosomes encapsulate circRNAs and lncRNAs in cancer cells. Exosomes are then discharged into the extracellular environment, where they are taken up by other cells. As a result, exosomal ncRNA cargo is critical for cell–cell communication within the cancer microenvironment. Exosomal ncRNAs can regulate a range of events, such as angiogenesis, metastasis, immune evasion, drug resistance, and epithelial-to-mesenchymal transition. To set the groundwork for developing novel therapeutic strategies against gastrointestinal malignancies, a thorough understanding of circRNAs and lncRNAs is required. In this review, we discuss the function and intrinsic features of oncogenic circRNAs and lncRNAs that are enriched within exosomes.
The Role of Tumor Microenvironment in the Pathogenesis of Sézary Syndrome
Authors: Miyashiro Denis , Souza Bruno de Castro e , Torrealba Marina Passos , Manfrere Kelly Cristina Gomes , Sato Maria Notomi , Sanches José Antonio
Published: January 2022

Sézary syndrome is an aggressive leukemic variant of cutaneous T-cell lymphomas, characterized by erythroderma, lymphadenopathy, and peripheral blood involvement by CD4+ malignant T-cells. The pathogenesis of Sézary syndrome is not fully understood. However, the course of the disease is strongly influenced by the tumor microenvironment, which is altered by a combination of cytokines, chemokines, and growth factors. The crosstalk between malignant and reactive cells affects the immunologic response against tumor cells causing immune dysregulation. This review focuses on the interaction of malignant Sézary cells and the tumor microenvironment.
Making Sense of “Nonsense” and More: Challenges and Opportunities in the Genetic Code Expansion, in the World of tRNA Modifications
Authors: Lateef Olubodun Michael , Akintubosun Michael Olawale , Olaoba Olamide Tosin , Samson Sunday Ocholi , Adamczyk Malgorzata
Published: January 2022

The evolutional development of the RNA translation process that leads to protein synthesis based on naturally occurring amino acids has its continuation via synthetic biology, the so-called rational bioengineering. Genetic code expansion (GCE) explores beyond the natural translational processes to further enhance the structural properties and augment the functionality of a wide range of proteins. Prokaryotic and eukaryotic ribosomal machinery have been proven to accept engineered tRNAs from orthogonal organisms to efficiently incorporate noncanonical amino acids (ncAAs) with rationally designed side chains. These side chains can be reactive or functional groups, which can be extensively utilized in biochemical, biophysical, and cellular studies. Genetic code extension offers the contingency of introducing more than one ncAA into protein through frameshift suppression, multi-site-specific incorporation of ncAAs, thereby increasing the vast number of possible applications. However, different mediating factors reduce the yield and efficiency of ncAA incorporation into synthetic proteins. In this review, we comment on the recent advancements in genetic code expansion to signify the relevance of systems biology in improving ncAA incorporation efficiency. We discuss the emerging impact of tRNA modifications and metabolism in protein design. We also provide examples of the latest successful accomplishments in synthetic protein therapeutics and show how codon expansion has been employed in various scientific and biotechnological applications.
The Action of Vitamin D in Adipose Tissue: Is There the Link between Vitamin D Deficiency and Adipose Tissue-Related Metabolic Disorders?
Authors: Szymczak-Pajor Izabela , Miazek Krystian , Selmi Anna , Balcerczyk Aneta , Śliwińska Agnieszka
Published: January 2022

Adipose tissue plays an important role in systemic metabolism via the secretion of adipocytokines and storing and releasing energy. In obesity, adipose tissue becomes dysfunctional and characterized by hypertrophied adipocytes, increased inflammation, hypoxia, and decreased angiogenesis. Although adipose tissue is one of the major stores of vitamin D, its deficiency is detective in obese subjects. In the presented review, we show how vitamin D regulates numerous processes in adipose tissue and how their dysregulation leads to metabolic disorders. The molecular response to vitamin D in adipose tissue affects not only energy metabolism and adipokine and anti-inflammatory cytokine production via the regulation of gene expression but also genes participating in antioxidant defense, adipocytes differentiation, and apoptosis. Thus, its deficiency disturbs adipocytokines secretion, metabolism, lipid storage, adipogenesis, thermogenesis, the regulation of inflammation, and oxidative stress balance. Restoring the proper functionality of adipose tissue in overweight or obese subjects is of particular importance in order to reduce the risk of developing obesity-related complications, such as cardiovascular diseases and diabetes. Taking into account the results of experimental studies, it seemed that vitamin D may be a remedy for adipose tissue dysfunction, but the results of the clinical trials are not consistent, as some of them show improvement and others no effect of this vitamin on metabolic and insulin resistance parameters. Therefore, further studies are required to evaluate the beneficial effects of vitamin D, especially in overweight and obese subjects, due to the presence of a volumetric dilution of this vitamin among them.
Glial Modulation of Energy Balance: The Dorsal Vagal Complex Is No Exception
Authors: Troadec Jean-Denis , Gaigé Stéphanie , Barbot Manon , Lebrun Bruno , Barbouche Rym , Abysique Anne
Published: January 2022

The avoidance of being overweight or obese is a daily challenge for a growing number of people. The growing proportion of people suffering from a nutritional imbalance in many parts of the world exemplifies this challenge and emphasizes the need for a better understanding of the mechanisms that regulate nutritional balance. Until recently, research on the central regulation of food intake primarily focused on neuronal signaling, with little attention paid to the role of glial cells. Over the last few decades, our understanding of glial cells has changed dramatically. These cells are increasingly regarded as important neuronal partners, contributing not just to cerebral homeostasis, but also to cerebral signaling. Our understanding of the central regulation of energy balance is part of this (r)evolution. Evidence is accumulating that glial cells play a dynamic role in the modulation of energy balance. In the present review, we summarize recent data indicating that the multifaceted glial compartment of the brainstem dorsal vagal complex (DVC) should be considered in research aimed at identifying feeding-related processes operating at this level.
Biphasic Functions of Sodium Fluoride (NaF) in Soft and in Hard Periodontal Tissues
Authors: Wang Xingzhi , Tewari Nitesh , Sato Fuyuki , Tanimoto Keiji , Thangavelu Lakshmi , Makishima Makoto , Bhawal Ujjal K.
Published: January 2022

Sodium fluoride (NaF) is widely used in clinical dentistry. However, the administration of high or low concentrations of NaF has various functions in different tissues. Understanding the mechanisms of the different effects of NaF will help to optimize its use in clinical applications. Studies of NaF and epithelial cells, osteoblasts, osteoclasts, and periodontal cells have suggested the significant roles of fluoride treatment. In this review, we summarize recent studies on the biphasic functions of NaF that are related to both soft and hard periodontal tissues, multiple diseases, and clinical dentistry.
Transcriptome Analysis Reveals Multiple Genes and Complex Hormonal-Mediated Interactions with PEG during Adventitious Root Formation in Apple
Authors: Li Shaohuan , Tahir Muhammad Mobeen , Wu Tong , Xie Lingling , Zhang Xiaoyun , Mao Jiangping , Ayyoub Anam , Xing Libo , Zhang Dong , Shao Yun
Published: January 2022

Adventitious root (AR) formation is a bottleneck for the mass propagation of apple rootstocks, and water stress severely restricts it. Different hormones and sugar signaling pathways in apple clones determine AR formation under water stress, but these are not entirely understood. To identify them, GL-3 stem cuttings were cultured on polyethylene glycol (PEG) treatment. The AR formation was dramatically decreased compared with the PEG-free control (CK) cuttings by increasing the endogenous contents of abscisic acid (ABA), zeatin riboside (ZR), and methyl jasmonate (JA-me) and reducing the indole-3-acetic acid (IAA) and gibberellic acid 3 (GA3) contents. We performed a transcriptomic analysis to identify the responses behind the phenotype. A total of 3204 differentially expressed genes (DEGs) were identified between CK and PEG, with 1702 upregulated and 1502 downregulated genes. Investigation revealed that approximately 312 DEGs were strongly enriched in hormone signaling, sugar metabolism, root development, and cell cycle-related pathways. Thus, they were selected for their possible involvement in adventitious rooting. However, the higher accumulation of ABA, ZR, and JA-me contents and the upregulation of their related genes, as well as the downregulation of sugar metabolism-related genes, lead to the inhibition of ARs. These results indicate that AR formation is a complicated biological process chiefly influenced by multiple hormonal signaling pathways and sugar metabolism. This is the first study to demonstrate how PEG inhibits AR formation in apple plants.
Reactions of Recombinant Neuronal Nitric Oxide Synthase with Redox Cycling Xenobiotics: A Mechanistic Study
Authors: Lesanavičius Mindaugas , Boucher Jean-Luc , Čėnas Narimantas
Published: January 2022

Neuronal nitric oxide synthase (nNOS) catalyzes single-electron reduction of quinones (Q), nitroaromatic compounds (ArNO2) and aromatic N-oxides (ArN → O), and is partly responsible for their oxidative stress-type cytotoxicity. In order to expand a limited knowledge on the enzymatic mechanisms of these processes, we aimed to disclose the specific features of nNOS in the reduction of such xenobiotics. In the absence or presence of calmodulin (CAM), the reactivity of Q and ArN → O increases with their single-electron reduction midpoint potential (E17). ArNO2 form a series with lower reactivity. The calculations according to an “outer-sphere” electron transfer model show that the binding of CAM decreases the electron transfer distance from FMNH2 to quinone by 1–2 Å. The effects of ionic strength point to the interaction of oxidants with a negatively charged protein domain close to FMN, and to an increase in accessibility of the active center induced by high ionic strength. The multiple turnover experiments of nNOS show that, in parallel with reduced FAD-FMN, duroquinone reoxidizes the reduced heme, in particular its Fe2+-NO form. This finding may help to design the heme-targeted bioreductively activated agents and contribute to the understanding of the role of P-450-type heme proteins in the bioreduction of quinones and other prooxidant xenobiotics.