bluuebunny/crossref_metadata_2025 huggingface dataset:
Laboratory information systems for research management in biology
Authors: Mukhin A. M. , Kazantsev F. V. , Lashin S. A.
Published: December 2023

Modern investigations in biology often require the efforts of one or more groups of researchers. Often these are groups of specialists from various scientific fields who generate and share data of different formats and sizes. Without modern approaches to work automation and data versioning (where data from different collaborators are stored at different points in time), teamwork quickly devolves into unmanageable confusion. In this review, we present a number of information systems designed to solve these problems. Their application to the organization of scientific activity helps to manage the flow of actions and data, allowing all participants to work with relevant information and solving the issue of reproducibility of both experimental and computational results. The article describes methods for organizing data flows within a team, principles for organizing metadata and ontologies. The information systems Trello, Git, Redmine, SEEK, OpenBIS and Galaxy are considered. Their functionality and scope of use are described. Before using any tools, it is important to understand the purpose of implementation, to define the set of tasks they should solve, and, based on this, to formulate requirements and finally to monitor the application of recommendations in the field. The tasks of creating a framework of ontologies, metadata, data warehousing schemas and software systems are key for a team that has decided to undertake work to automate data circulation. It is not always possible to implement such systems in their entirety, but one should still strive to do so through a step­by­step introduction of principles for organizing data and tasks with the mastery of individual software tools. It is worth noting that Trello, Git, and Redmine are easier to use, customize, and support for small research groups. At the same time, SEEK, OpenBIS, and Galaxy are more specific and their use is advisable if the capabilities of simple systems are no longer sufficient.
Analysis of the transcriptional activity of model piggyBac transgenes stably integrated into different loci of the genome of CHO cells in the absence of selection pressure
Authors: Yarinich L. A. , Ogienko A. A. , Pindyurin A. V. , Omelina E. S.
Published: December 2023

CHO cells are most commonly used for the synthesis of recombinant proteins in biopharmaceutical production. When stable producer cell lines are obtained, the locus of transgene integration into the genome has a great influence on the level of its expression. Therefore, the identification of genomic loci ensuring a high level of protein production is very important. Here, we used the TRIP assay to study the influence of the local chromatin environment on the activity of transgenes in CHO cells. For this purpose, reporter constructs encoding eGFP under the control of four promoters were stably integrated into the genome of CHO cells using the piggyBac transposon. Each individual transgene contained a unique tag, a DNA barcode, and the resulting polyclonal cell population was cultured for almost a month without any selection. Next, using the high-throughput sequencing, genomic localizations of barcodes, as well as their abundances in the population and transcriptional activities were identified. In total, ~640 transgenes more or less evenly distributed across all chromosomes of CHO cells were characterized. More than half of the transgenes were completely silent. The most active transgenes were identified to be inserted in gene promoters and 5’ UTRs. Transgenes carrying Chinese hamster full-length promoter of the EF-1α gene showed the highest activity. Transgenes with a truncated version of the same promoter and with the mouse PGK gene promoter were on average 10 and 19 times less active, respectively. In total, combinations of genomic loci of CHO cells and transgene promoters that together provide different levels of transcriptional activity of the model reporter construct were described.
FROM THE EDITOR
Authors: Kolchanov N. A. , Matushkin Yu. G.
Published: December 2023

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The central regulatory circuit in the gene network controlling the morphogenesis of Drosophila mechanoreceptors: an in silico analysis
Authors: Bukharina T. A. , Golubyatnikov V. P. , Furman D. P.
Published: December 2023

Identification of the mechanisms underlying the genetic control of spatial structure formation is among the relevant tasks of developmental biology. Both experimental and theoretical approaches and methods are used for this purpose, including gene network methodology, as well as mathematical and computer modeling. Reconstruction and analysis of the gene networks that provide the formation of traits allow us to integrate the existing experimental data and to identify the key links and intra-network connections that ensure the function of networks. Mathematical and computer modeling is used to obtain the dynamic characteristics of the studied systems and to predict their state and behavior. An example of the spatial morphological structure is the Drosophila bristle pattern with a strictly defined arrangement of its components – mechanoreceptors (external sensory organs) – on the head and body. The mechanoreceptor develops from a single sensory organ parental cell (SOPC), which is isolated from the ectoderm cells of the imaginal disk. It is distinguished from its surroundings by the highest content of proneural proteins (ASC), the products of the achaete-scute proneural gene complex (AS-C). The SOPC status is determined by the gene network we previously reconstructed and the AS-C is the key component of this network. AS-C activity is controlled by its subnetwork – the central regulatory circuit (CRC) comprising seven genes: AS-C, hairy, senseless (sens), charlatan (chn), scratch (scrt), phyllopod (phyl), and extramacrochaete (emc), as well as their respective proteins. In addition, the CRC includes the accessory proteins Daughterless (DA), Groucho (GRO), Ubiquitin (UB), and Seven-in-absentia (SINA). The paper describes the results of computer modeling of different CRC operation modes. As is shown, a cell is determined as an SOPC when the ASC content increases approximately 2.5-fold relative to the level in the surrounding cells. The hierarchy of the effects of mutations in the CRC genes on the dynamics of ASC protein accumulation is clarified. AS-C as the main CRC component is the most significant. The mutations that decrease the ASC content by more than 40 % lead to the prohibition of SOPC segregation.
Molecular-genetic pathways of hepatitis C virus regulation of the expression of cellular factors PREB and PLA2G4C, which play an important role in virus replication
Authors: Mishchenko E. L. , Makarova A. A. , Antropova E. A. , Venzel A. S. , Ivanisenko T. V. , Demenkov P. S. , Ivanisenko V. A.
Published: December 2023

The participants of Hepatitis C virus (HCV) replication are both viral and host proteins. Therapeutic approaches based on activity inhibition of viral non-structural proteins NS3, NS5A, and NS5B are undergoing clinical trials. However, rapid mutation processes in the viral genome and acquisition of drug resistance to the existing drugs remain the main obstacles to fighting HCV. Identifying the host factors, exploring their role in HCV RNA replication, and studying viral effects on their expression is essential for understanding the mechanisms of viral replication and developing novel, effective curative approaches. It is known that the host factors PREB (prolactin regulatory element binding) and PLA2G4C (cytosolic phospholipase A2 gamma) are important for the functioning of the viral replicase complex and the formation of the platforms of HCV genome replication. The expression of PREB and PLA2G4C was significantly elevated in the presence of the HCV genome. However, the mechanisms of its regulation by HCV remain unknown. In this paper, using a text-mining technology provided by ANDSystem, we reconstructed and analyzed gene networks describing regulatory effects on the expression of PREB and PLA2G4C by HCV proteins. On the basis of the gene network analysis performed, we put forward hypotheses about the modulation of the host factors functions resulting from protein-protein interaction with HCV proteins. Among the viral proteins, NS3 showed the  greatest number of regulatory linkages. We assumed that NS3 could inhibit the function of host transcription factor (TF) NOTCH1 by protein-protein interaction, leading to upregulation of PREB and PLA2G4C. Analysis of the gene networks and data on differential gene expression in HCV-infected cells allowed us to hypothesize further how HCV could regulate the expression of TFs, the binding sites of which are localized within PREB and PLA2G4C gene regions. The results obtained can be used for planning studies of the molecular-genetic mechanisms of viral-host interaction and searching for potential targets for anti-HCV therapy.
Intratumor heterogeneity: models of malignancy emergence and evolution
Authors: Ivanov R. A. , Lashin S. A.
Published: December 2023

Cancer is a complex and heterogeneous disease characterized by the accumulation of genetic alterations that drive uncontrolled cell growth and proliferation. Evolutionary dynamics plays a crucial role in the emergence and development of tumors, shaping the heterogeneity and adaptability of cancer cells. From the perspective of evolutionary theory, tumors are complex ecosystems that evolve through a process of microevolution influenced by genetic mutations, epigenetic changes, tumor microenvironment factors, and therapy­induced changes. This dynamic nature of tumors poses significant challenges for effective cancer treatment, and understanding it is essential for developing effective and personalized therapies. By uncovering the mechanisms that determine tumor heterogeneity, researchers can identify key genetic and epigenetic changes that contribute to tumor progression and resistance to treatment. This knowledge enables the development of innovative strategies for targeting specific tumor clones, minimizing the risk of recurrence and improving patient outcomes. To investigate the evolutionary dynamics of cancer, researchers employ a wide range of experimental and computational approaches. Traditional experimental methods involve genomic profiling techniques such as next­generation sequencing and fluorescence in situ hybridization. These techniques enable the identification of somatic mutations, copy number alterations, and structural rearrangements within cancer genomes. Furthermore, single­cell sequencing methods have emerged as powerful tools for dissecting intratumoral heterogeneity and tracing clonal evolution. In parallel, computational models and algorithms have been developed to simulate and analyze cancer evolution. These models integrate data from multiple sources to predict tumor growth patterns, identify driver mutations, and infer evolutionary trajectories. In this paper, we set out to describe the current approaches to address this evolutionary complexity and theories of its occurrence.
TWO NEW LOCALITIES FOR THE SPOTTED BETTA BETTA PICTA (VALENCIENNES 1846) (ANABANTIFORMES, OSPHRONEMIDAE) IN JAVA, INDONESIA
Authors: Hasan Veryl , Samitra Dian , Dewi Masithah Endang , Sri Widodo Maheno , Irwanjasmoro Susilo , Vieira Lucas de Oliveira , Ottoni Felipe Polivanov
Published: December 2023

For the first time, the species Betta picta is registered for the southern part of Central Java (Indonesia), being recorded for two localities in the Progo River basin catchment, one in the Magelang and the other in the Bantul Regency, representing an extension of about 100km south to the nearest previously known distribution. These two new records were based on four specimens, three males and one female, collected in February 2019, which were identified as Betta picta since the males possess the main diagnostic features of the species: opercle with yellow-gold iridescence coloration, interrupted stripe below eyes, and a dark subdistal band on caudal and anal fins. Currently, this species is categorized as Near Threatened (NT) by the IUCN, and considering the negative effects of urbanization over the species natural habitats, the new records are important for the species conservation.
INFLUÊNCIA DO ESTILO PARENTAL PERMISSIVO-INDULGENTE NA CONSTRUÇÃO DO PERSONAGEM BRÁS CUBAS
Authors: Moraes Daniella Furtado , Silva Natália Leitão Barros da , Bonfim Maria Aracy
Published: December 2023

Este artigo tem como objetivo estudar a influência do estilo parental, mais necessariamente o estilo permissivo pautado nas teóricas Albuquerque (2016), Baumrind (1966) e Weber (2009), no comportamento de Brás Cubas, no livro Memórias Póstumas de Brás Cubas, escrito por Machado de Assis. Antes de adentrar na análise da influência que os pais tiveram na vida de Brás Cubas, este artigo vai analisar como é feita a construção de um personagem seguindo como embasamento teórico Antonio Candido (1976) e Elvis Couto (2019), a análise da criação do personagem explica as distinções dos diferentes modos de agir de um indivíduo e de um personagem de ficção, que possui toda sua complexidade delimitada pelo autor. Para fazer o presente estudo sobre esse artigo, foram feitas diversas pesquisas bibliográficas mediante os diversos tipos de gênero e estilos parentais, bem como as influências que esses pais possuem no desenvolvimento do sujeito, e também foi necessário estudar profundamente o que seria um personagem de Ficção, principalmente indo para a perspectiva apresentada por Antônio Candido sobre o que seria um personagem de romance e como é feita a construção desse personagem de romance, que no caso deste artigo analisa-se todo o processo de formação e influência de Brás Cubas.
Entre o nacional e o internacional: a edição de livros de culinária e gastronomia no Brasil
Authors: Previatti Débora
Published: December 2023

Na virada do século XX para o século XXI, há um boom na produção de livros de culinária e gastronomia no Brasil, passando a compor fração representativa do mercado editorial brasileiro. A fim de compreender características de tal expansão, foram realizadas entrevistas em profundidade com editores e consultados os acervos da Fundação Biblioteca Nacional e do Sistema Integrado de Bibliotecas da Universidade de São Paulo. Foram analisados os determinantes sociais de uma produção cosmopolita desde a origem, a partir da qual constituiu-se um gênero editorial e um subcampo relativamente autônomo, com suas próprias hierarquias e classificações. Múltiplos agentes passaram a repercutir na feição e no sentido desses livros, nos quais a fotografia e a estética ganharam ampla evidência.
Leis nº 10.639/2003 e nº 11.645/2008 e educação antirracista: ideias de um grupo de docentes de química
Authors: Lima Analice Almeida
Published: December 2023

O presente estudo teve como objetivo analisar as ideias de docentes de química acerca das possibilidades e limitações em adotar uma perspectiva antirracista no ensino de química. Na discussão teórica do trabalho, partimos, principalmente, do referencial de Silvio Almeida e de Neusa Santos Souza nas discussões sobre o racismo, e do referencial de Eliane Cavalleiro sobre educação antirracista. Os/as participantes foram 19 mestrandos/as do Programa de Mestrado Profissional em Química em Rede Nacional da Universidade Federal Rural de Pernambuco, disciplina de Seminários Web 2, ministrada de modo remoto. Como instrumento de pesquisa, utilizamos o Google Forms contendo 15 perguntas divididas em sete questões relacionadas à caracterização dos/as participantes, sete perguntas concernentes ao objeto de pesquisa e a autorização dos/das participantes para utilização dos dados da pesquisa. Embora as discussões sobre a temática tenham sido destacadas como importantes nas respostas, observamos a pouca aproximação com as questões relacionadas às Leis nº 0.639/2003 e nº 11.645/200 e à educação antirracista. As temáticas citadas pelo grupo para subsidiar uma abordagem antirracista no ensino de química foram cor da pele e estruturas químicas, cientistas negros e negras na história da química, tintas naturais utilizadas no continente africano e ervas medicinais e culinária indígena.
Laws nº 10.639/2003 and nº 11.645/2008 and anti-racist education: ideas of a group of chemistry teachers
Abstract
This study aimed to analyze the ideas of chemistry teachers about the possibilities and limitations of adopting an anti-racist perspective in the teaching of chemistry. In the theoretical discussion of the study, we adopted, mainly, the references of Silvio Almeida and Neusa Santos Souza in discussions about racism and the reference of Eliane Cavalleiro on anti-racist education. The participants were 19 master students from Programa de Mestrado Profissional em Química em Rede Nacional da Universidade Federal Rural de Pernambuco during the course Seminários Web 2, taught remotely. As a research instrument, we used Google Forms containing 15 questions divided into seven questions related to the characterization of the participants, seven questions concerning the research object, and the authorization of the participants for the use of the research data. Although the discussions on the theme were highlighted as important in the answers, we observed little approximation to the issues related to Laws 10.639/2003 and 11.645/200 and anti-racist education. The themes mentioned by the group to support an anti-racist approach in the teaching of chemistry were skin color and chemical structures, black scientists in the history of chemistry, natural dyes used on the African continent, and medicinal herbs and indigenous cuisine.
Civic budget and its role in the process of urban reconstruction - a case study of the city of Lodz
Authors: Ślebocka Magdalena , Kilanowski Artur
Published: December 2023

The purpose of the article/hypothesis: The aim of the article is to assess the role and importance of the civic budget in a broader processes of reconstruction of the degraded urban fabric in the period 2016–2023. The article poses a research hypothesis that the civic budget, as an advanced form of social participation not limited to information or public consultation, is a tool supporting the implementation of revitalization processes undertaken in the city. The research questions were helpful in verifying the proposed hypothesis: 1) Does the civic budget support revitalization processes? 2) What types of projects submitted as part of the civic budget are included in revitalization measures? 3) How is the tool that the civic budget is in revitalization perceived by stakeholders and initiators of these processes?

Methodology: The research involved a literature review and an analysis of numerical data and information on revitalization projects that can be found in the civic budget of Lodz. The study also used an original survey questionnaire conducted among the residents.

Results of the research: It was found that the civic budget in Lodz contributed to the implementation of a number of revitalization projects. The high civic awareness of the residents and their knowledge of the needs of their surroundings make it possible to implement these projects in areas where the condition of the urban fabric is critical. Thanks to the civic budget, the city's residents have had and continue to have an opportunity to make changes in their immediate environment. They can realize the most important investments from the point of view of local needs.
Glaucoma neovascular, una de las consecuencias de la oclusión de la vena central de la retina
Authors: Jiménez Rojas Nicol Daniela
Published: December 2023

Objetivo. Estudiar y describir la relación entre la oclusión de la vena central de la retina y el glaucoma neovascular, su etiología, epidemiología, fisiopatología, factores de riesgo, presentación clínica y tratamientos. Metodología. Revisión de la literatura acerca del glaucoma neovascular secundario a oclusión de la vena central de la retina, tomando artículos comprendidos entre 2014 y 2022, con criterios de exclusión artículos de series de casos. Resultados. El glaucoma neovascular (GNV) es una afectación patológica ocular, secundaria a una afección ocular y/o sistémica hipóxica que conduce a isquemia retiniana, dentro de las etiologías más comunes (33 %) se encuentra la oclusión de la vena central de la retina (OVCR) una retinopatía vascular común que puede presentarse como isquémica o no isquémica. Discusión de resultados. La OVCR ocurre secundariamente a cambios estructurales glaucomatosos o coexiste con la anormalidad hemodinámica de la retina, esta es mediada por procesos inflamatorios como consecuencia de la isquemia retiniana y la hemorragia, lo cual aumenta la expresión de factor de crecimiento endotelial vascular (VEGF) y otros factores inflamatorios en el ojo, que pueden llevar a GNV. Conclusiones. El GNV es una patología con una presentación clínica severa, se asocia de forma directa con la OVCR y tiene múltiples opciones de tratamientos.
Production of Broccoli on No-Tillage and Surrounding Range
Authors: Antunes Darley Tiago
Published: December 2023

The broccoli (bráassic oleracea var. ittheLica) is a kind of great consumer demand and attractive price to producers in Mato Grosso. The present Work had as objective to develop a cultivation system using no-tillage and surrounding range, aiming to improve the production of broccoli. The experiment was conducted open Field and between the period of17/07/2016 to 31/12/2016.In the plots were evaluated the presence and absence of the surrounding range with Sorghum broom (sorghum vulgare) and in the subplots were evaluated four soil covers on no-tillage of Millet (pennisetum Glaujizz), sorghum (sorghum bicolor), Crow’s foot grass (elusive indica), fallow (spontaneous species) and conventional cultivation with soil revolting. The parameters evaluated were fresh mass of leaves, stem and inflorescence (production - kg Plant-1), inflorescence and stem diameter at inflorescence insertion (cm), number of leaves and visual aspect index of Inflorescences. The type of soil cover did not influence any of the evaluated parameters. The use of the surrounding range provided better results for fresh stem mass, inflorescence diameter and fresh inflorescence mass.
Production of A Mors Drink with A Natural Sweetener
Authors: Senchenko Marina
Published: December 2023

Nowadays many countries are actively replacing sugar in the formulations of different products, due to the need to optimize human nutrition, as well as the ability to address the issues of rational nutrition of people with certain diseases. In everyday life, people prefer non-alcoholic beverages because of their ability to cover the body’s need for water, quench thirst and ease of use. One of the most preferred non-carbonated non-alcoholic beverages is moors, in the formulation of which sucrose, an undesirable component for people living with certain diseases, such as obesity, diabetes mellitus, etc., is used. The purpose of our research was to study the possibility of using dry stevia leaves [Stevia rebaudiana BERTONI (L.)] (hereinafter stevia) as a sugar substitute and traditional local vegetable raw material in the formulation when producing a moors drink. The results of the evaluation of organoleptic and physico-chemical indicators, the content of macro-and microelements in the moors drink produced from different formulations are presented. In order to use domestic, traditional local vegetable raw material containing a wide range of substances of different pharmacological orientation and in order to adjust organoleptic indicators, additional components of the prescription set (dry peppermint grass, Melissa, organum) were used. The use of traditional local vegetable raw material and a stevia sweetener in the production of a moors drink expands the range of this product in the world industry of non-alcoholic beverages.
Os cientistas sociais no topo da hierarquia acadêmica
Authors: Oliveira Amurabi
Published: December 2023

Introdução: As ciências sociais brasileiras têm realizado um exercício contínuo de autorreflexão, principalmente a partir de sua institucionalização e da relação entre as disciplinas. Objetivo: Visando contribuir para a análise desse campo, este artigo objetiva examinar o perfil dos bolsistas de Produtividade em Pesquisa do CNPQ nível 1A das Ciências Sociais (Antropologia, Ciência Política e Sociologia), examinando suas trajetórias acadêmicas e atuação profissional. Metodologia: Os dados utilizados foram coletados no site do CNPQ e na plataforma lattes, buscando realizar uma prosopografia desse grupo, analisando-o a partir da teoria dos campos. Resultados: Observou-se uma forte concentração regional e geracional entre esses pesquisadores, com uma relevante inserção na burocracia acadêmica e em associações científicas, além de uma intensa circulação internacional. Conclusão: Apesar de haver um cenário relativamente similar entre as três áreas, há algumas diferenças no perfil dos pesquisadores principalmente com relação à distribuição geográfica, geração e formação acadêmica.
A importância da dimensão da cultura nos processos de qualificação em turismo nos territórios brasileiros da Rota Bioceânica
Authors: Bassinello Patricia Zaczuk , Souza Carla Cristina de , Arruda Dyego de Oliveira , Mariani Milton Augusto Pasquotto
Published: December 2023

O presente artigo tem como objetivo propor diretrizes de um programa de qualificação em turismo voltado para os municípios brasileiros sob influência da Rota Bioceânica, tendo como premissa o respeito das especificidades culturais do território e a respectiva participação das comunidades nesse processo. Em termos metodológicos, partimos de uma abordagem qualitativa, na qual conduzimos um conjunto de seis rodas de conversa com diversos atores que estão em contato permanente com as comunidades no território. No que tange à perspectiva teórico-analítica empregada na construção do artigo, nos valemos dos Estudos Culturais, Pós-Coloniais e decoloniais, tendo como premissa pensar, propor e contribuir com iniciativas de qualificação em turismo que rompam com a lógica da colonialidade e, portanto, auxiliem nos processos de emancipação das comunidades e afirmação de suas manifestações culturais. Dessa maneira, o artigo parte de um eixo central – que denominamos de Eixo Cultura – e sugere um conjunto de cinco diretrizes que podem ser centrais na difusão de iniciativas de qualificação em turismo junto às comunidades no território brasileiro da Rota Bioceânica. Esperamos, com as reflexões presentes neste manuscrito, contribuir para visibilizar a realidade das comunidades no entorno da Rota Bioceânica, chamando a atenção para a importância de que essas comunidades estejam plenamente contempladas em todas as dinâmicas que envolvem as atividades direta ou indiretamente estimuladas pela Rota Bioceânica, incluindo, nesta seara, a própria atividade do turismo.
Mato Grosso do Sul na Rota do Crescimento: uma investigação sobre as potencialidades impulsionadas pela Rota Bioceânica
Authors: Constantino Michel , Da Costa Reginaldo Brito , Bolson Daniel Silva , Mendes Dany
Published: December 2023

O presente artigo objetivou investigar a relação entre as potencialidades do crescimento econômico de Mato Grosso do Sul com a implementação da Rota Bioceânica. Utilizando dos estudos realizados e publicados desde 2015, a análise estrutura e define as principais potencialidades no âmbito macroeconômico e setorial para Mato Grosso do Sul. Os resultados definiram cinco pontos de análise: i. Crescimento econômico acelerado; ii. Ampliação do comércio internacional; iii. Investimento em infraestrutura; iv. Desenvolvimento de novas cadeias produtivas e v. Fortalecimento da posição estratégica do estado. Foi elaborado uma análise de radar e a distribuição de probabilidade de impacto em cada ponto elencado após a implementação da Rota em definitivo, o que pode levar o estado de Mato Grosso do Sul para a Rota do Crescimento.
A governança do turismo em fronteira internacional: políticas públicas e paradiplomacia no contexto da RILA
Authors: Silva Fabio Roberto Cordeiro da , Grechi Dores Cristina , Carneiro Camilo Pereira
Published: December 2023

A Rota de Integração Latino-Americana (RILA), também conhecida como Rota Bioceânica, é uma iniciativa com o potencial de promover o desenvolvimento da zona de fronteira Brasil-Paraguai. Os idealizadores da RILA objetivam, desde o surgimento da proposta em 2006 (que seria oficializada em 2015), impulsionar a atividade logística, com vistas a aumentar as exportações de commodities (grãos, minérios e carnes) e, em segundo lugar, potencializar a atividade turística regional.  Nesse sentido, este artigo visou analisar as potencialidades e obstáculos que envolvem a governança do turismo na fronteira entre Brasil e Paraguai, especificamente nas cidades gêmeas de Porto Murtinho (BR) e Carmelo Peralta (PY). Em relação à metodologia, o presente trabalho configura uma pesquisa qualitativa, pautada em análise bibliográfica e documental, sob uma ótica interdisciplinar, ancorada nos referenciais do Turismo e da Geografia Política. Os resultados teórico-empíricos correspondem a delimitação conceitual do que se configura a governança do turismo em territórios de fronteira internacional e na organização da matriz institucional que dará subsídio à compreensão da organização do turismo e de sua governança no território pesquisado. De forma complementar, foram elaborados mapas que demonstram a intersecção entre a principal política de desenvolvimento do turismo em Mato Grosso do Sul e a RILA.
Os povos da Rota de Integração Latino Americana (RILA): interseções turísticas
Authors: Leonel Waldir , Ferreira Magali Luzio , Castilho Maria Augusta
Published: December 2023

O presente artigo ressalta a ocupação dos primeiros e inúmeros povos, como as diversas etnias indígenas, em especial os povos guarani, os ameríndios incas (os quéchua), uruguaios, austríacos, bolivianos, espanhóis, britânicos, croatas, entre outros. que se deslocaram para os países que contribuem para a edificação da Rota Bioceânica Latino Americana, com enfoque em seus costumes, crenças e proliferações culturais. Com efeito as realizações destes povos permitem visualizar e entender a simbologia local que dá o tom e a forma do desenrolar do projeto Bioceânico que tem como objetivo a redução do tempo para a chegada de mercadorias, facilitar a exportação e fomentar turismo regional. O cenário que perpassa a rota foi elaborado por migrantes que ajustaram suas histórias a mobilidade turística, que caminha com o setor econômico. Ocupou-se desta análise a espacialidade, a história, as mudanças e permanências ocorridas com o tempo. As argumentações teórico-metodológicas estão presentes nos estudos de autores que se debruçam nos assuntos, tais qual: patrimônio cultural material/imaterial, memória, espaço, lugar, território/territorialidade, reterritorialidade e desenvolvimento local. Desse modo, esta seara acadêmica proporciona esteio para os estudos e pesquisas em integração de povos, vocações turísticas e consequentemente desenvolvimento local.
Às margens da rota: discursos sobre o desenvolvimento na cobertura jornalistica da RILA
Authors: Machado Flavia Cristina Albuquerque Palhares , Maciel Josemar de Campos
Published: December 2023

Grandes projetos de infraestrutura são frequentemente apresentados e justificados como estratégias para o desenvolvimento. Entretanto, muitos dos resultados experienciados diretamente pelos afetados por estes projetos em seus cotidianos são diferentes dos objetivos previamente definidos. Partindo da ideia de desenvolvimento como um processo consistente, desejado e promovido de transformações sociais e econômicas em direção a ao progresso e à modernidade, buscamos identificar, a partir do noticiário sobre a Rota de Integração Latino Americana (RILA), os significados e ideias expressos sobre o desenvolvimento vinculados ao projeto e implantação da Rota Bioceânica. Este trabalho, portanto, tem como objetivo refletir sobre os aspectos discursivos implícitos ao projeto da RILA e a sua implantação a partir da análise de quarenta e nove reportagens veiculadas no portal G1 sobre a sua implementação publicados no período entre 23/08/2017 e 04/06/2023. A análise permite inferir que a RILA não é tratada como um projeto nacional de desenvolvimento mais amplo pelo governo Brasileiro que envolve além dos aspectos diplomáticos, produtivos e de infraestrutura também aspectos sociais, culturais, ambientais e legais enquanto agentes econômicos interessados na concretização do projeto atuam diretamente no projeto e desenho das práticas e estratégias a serem adotadas para promover o desenvolvimento.
Rede de pesquisa em turismo: conexões, prospecções e desafios para a sustentabilidade na Rota de Integração Latino-Americana
Authors: Mamede Simone , Amaral Vivianne , Benites Maristela
Published: December 2023

O presente trabalho tem como objetivo apresentar as conexões dos elos do eixo de Turismo e Desenvolvimento Local, de forma a evidenciar a malha e a dinamicidade de uma rede que emerge no campo de interações potenciais da UniRila (Rede Universitária da Rota de Integração Latino-Americana), tecida por pesquisadores e parceiros que atuam na investigação do turismo e desenvolvimento local na região da Rota de Integração Latino-Americana, também conhecida como Rota Bioceânica. Para a análise foram considerados os elos (atores sociais), parcerias institucionais, produtos e tipo de atividade da Rede de Pesquisa em Turismo na Rota de Integração Latino-Americana no período de 2018 a 2020. Para a representação gráfica da Rede foi utilizado o programa NodeXL Basic Framework 3.5. Nessa tecitura de rede, tudo é social e os elos são caracterizados por atores sociais/pessoas (pesquisadores, acadêmicos, profissionais do turismo local ou do território comum a todos) atuantes na Rota de Integração Latino-Americana. Ao todo, foram identificados 16 produtos sociais resultantes de ações cooperativas entre 24 instituições. As conexões são as relações sociais entre esses atores e os fluxos existentes estão relacionados aos produtos sociais resultantes dessas relações, como projetos, pesquisas, artigos e outras ações como lives via redes de computadores, workshops e seminários relacionados ao turismo na Rota de Integração Latino-Americana.
Technology Acceptance Model of ATM Machine Services: A Case Study of MyGraPARI within Analytical Hierarchy Process
Authors: Sutresno  , Affandi Achmad
Published: December 2023

Determining the priority level of acceptance of the MyGraPARI ATM machine service technology at Jam Gadang Bukit Tinggi is crucial for enhancing user satisfaction and streamlining financial transactions. The implementation of the Analytic Hierarchy Process (AHP) framework can effectively identify and prioritize factors that influence user acceptance and satisfaction. AHP systematically decomposes the decision-making process into a hierarchical structure, enabling the evaluation of various criteria based on their relative importance. AHP quantifies the subjective judgments of decision-makers, resulting in a comprehensive understanding of the factors that drive user acceptance and satisfaction. This predictive capability enables decision-makers to select the most effective strategies, optimizing the overall user experience and maximizing the adoption of MyGraPARI ATM machines at Jam Gadang Bukit Tinggi. In conclusion, the application of the AHP method is highly relevant and beneficial for overcoming existing challenges and enhancing the value proposition of MyGraPARI ATM machines. By prioritizing critical factors, implementing targeted strategies, and evaluating the impact of improvement efforts, AHP empowers decision-makers to optimize user acceptance and satisfaction, leading to a more successful and impactful MyGraPARI ATM service at Jam Gadang Bukit Tinggi.
Influence of Halal Lifestyle, Islamic Branding, and Social Media Marketing on Muslim Fashion Purchasing Decisions
Authors: Mutmainah  , Romadhon Muhammad Ryan
Published: December 2023

This study aim to analyze the influence of halal lifestyle, Islamic branding, social media marketing on Muslim fashion purchasing decisions in East Java. The current halal lifestyle trend also affects the consumption pattern of society, including Muslim fashion trends that are always developed. The development of information technology affects the economic activities carried out by community, as well as halal lifestyle indicates the increasing awareness of Muslims in making purchase decisions should be based on religiosity. This research uses a quantitative approach within multiple linear regression methods using Eviews 9 statistical tool. Data obtained by consumer surveys, the research was conducted online in August 2023 with 101 respondents in East Java. The result showed halal lifestyle variable does not have a significant effect on the purchase decision variable. Furthermore, Islamic branding and social media marketing variable are a significant effect on the purchase decision variable. In purchasing Muslim fashion products, not all consumers pay attention to halal lifestyle because sometimes in determining the purchase of a Muslim fashion product consumer think more about brands and products that are trending on social media. Thus, this research bring any practical implication for Muslim fashion business to strengthen promotion and communication through social media that has a greater likelihood of marketing than through traditional advertising channels.
DIFFERENCES IN PARENTS’ READINESS TO ACCEPT CHILDREN’S COVID-19 VACCINATION IN PATI REGENCY
Authors: Verganza Margaretha Inadyas , Sriatmi Ayun , Nandini Nurhasmadiar
Published: December 2023

Background: The coverage of COVID-19 vaccination for children in Pati Regency was still low at 37% in February 2022 compared to the 75% target. Some preliminary studies in Kudus showed that parents who received socialization about children’s COVID-19 vaccination experienced concerns affecting their responses negatively to the program.
Aims: This study aims to analyze differences in parental acceptance of the COVID-19 vaccination for children in Pati Regency.
Methods: This study was quantitative research with a cross-sectional approach. The variables consisted of 7Cs components (Confidence, Complacency, Constraints, Calculation, Collective Responsibility, Compliance, Conspiracy) and acceptance of COVID-19 vaccination. The total sample of respondents was 372 parents divided into two independent groups obtained by cluster and proportional sampling. Data were collected using a questionnaire via Google Forms and analyzed using the Mann-Whitney test.
Results: The results showed differences in parental acceptance of COVID-19 vaccination (sig = 0.006) regarding constraints (sig = 0.000), collective responsibility (sig = 0.012), compliance (sig = 0.012), confidence (sig = 0.019), complacency (sig = 0.020), calculation (sig = 0.027), and conspiracy (sig = 0.037).
Conclusion: Some differences were found between parents whose children received the vaccine and those whose children did not receive the COVID-19 vaccine. Parents whose children received the COVID-19 vaccine were likely to have a good perception of the COVID-19 vaccination. Meanwhile, parents whose children did not get vaccinated would likely have a bad perception. Educational and consulting services possibly increase vaccination coverage.
Keywords: COVID-19 vaccination for children; different acceptance; 7Cs components
DIFFERENT ROUTES FOR THE SYNTHESIS OF BENZALDEHYDE-BASED DIHYDROPYIMIDINONES VIA BIGINELLI REACTION
Authors: Ilfahmi Yan Alamanda , Fadlan Arif
Published: December 2023

Multicomponent reactions involving three or more reactants are commonly used to prepare dihydropyrimidinone with various bioactivities. This study reports the different routes for the synthesis of benzaldehyde-based dihydropyrimidinone via the Biginelli reaction and investigates the yield of the obtained products. The synthesis was performed via routes A, B, C, D, and E based on the formation of iminium, enamine, and Knoevenagel intermediates between urea, benzaldehyde, and ethyl acetoacetate. Route A, through a one-pot reaction via iminium, produced dihydropyrimidinone with a yield of 58%. The product from route B via iminium was obtained in 62% yield. Route C and D occurred via enamine at room temperature, and reflux gave the product 31% and 40% yield, respectively. Route E involving Knoevenagel intermediate provided the product in a 38% yield. 1H NMR, FTIR, and MS spectroscopic techniques were used for structure elucidation.
DEGRADATION OF TETRACYCLINE BY FLOATING PHOTOCATALYST TiO2/Ni-COCONUT FIBER
Authors: Putri Lavena Imelda , Agustiawan Deri , Sugandi Didiek , Arfida Khaizurani , Mardhatilla  , Wahyuni Nelly
Published: December 2023

The photocatalyst process involves light (photons) as an energy source and catalysts such as TiO2 to accelerate the reaction. Efforts are made to reduce the band gap energy of TiO2 by shifting the absorption towards visible light using metal cation doping, such as Ni2+, and they can float on the surface with coconut fiber. XRD characteristics with TiO2 diffractogram experienced a 2θ shift as an indication that Ni has entered the TiO2 structure and seen some peaks decreased in intensity after being embedded with coconut fiber as an indication that TiO2/Ni has successfully attached to the fiber. The band gap energy on TiO2 is 3.21 eV with a wavelength of 386.5 nm in UV light. TiO2/Ni-coconut fiber experienced a shift in band gap energy to 3.09 eV with a wavelength of 400.9 nm, which is in visible light. This indicates that Ni has successfully entered the TiO2 structure. The TiO2/Ni catalyst embraced with coconut fiber has a higher degradation activity than the catalyst without an embrainer, with a percent degradation of 28.66% for 120 minutes of irradiation. This is influenced by the amount of light that can be absorbed during the photocatalysis process.
Prof. Dr. GÖKHAN HOTAMIŞLIGİL: Nobel’e doğru yürüyüşüne tanık olduğum Bir Bilim Adamı
Authors: AKAR Nejat
Published: December 2023

Prof. Dr. Gökhan Hotamışlıgil, obezite, diyabet, karaciğer yağlanması ve kalp-damar hastalıkları gibi halk sağlığı için sorun oluşturan ve sık görülen kronik metabolik hastalıkların temel mekanizmalarını aydınlatmaya yönelik bilimsel buluşlara imza atmış bir bilim adamıdır.

Onun alanında çalışmayan ama gerçekleştirdiği buluşlarla Nobel Ödülüne yürüyüşüne şahit olan bir öğretim üyesiyim. Onu ilk kez tanıdığım 1987 yılından itibaren kesişen yollarımızın bir özetiyle, taşıdığı bir bilim adamında olması gereken özelliklerini, benim gözümden bu yazıda okuyacaksınız.
Prof. Dr. Türkan Saylan, Yönderim
Authors: YÜKSEL Ayse
Published: December 2023

Özet

13 Aralık 1935 yılında, İstanbul’un Kandilli semtinde Leyla-Fasih Saylan çiftinin ilk çocuğu olarak dünyaya gelmişti. Sonra da üç oğlan bir kız kardeşi daha oldu, beş kardeş bahçeli bir evde gönüllerince çocukluk yaşadılar. İlk, orta eğitimini Kandilli’de aldı, sonraki yıllarda Kandilli Kız Lisesi Vakfı Başkanlığı da yaptığı Kandilli Kız Lisesinde, gündüzlü eğitim görürken, arkadaşları ve öğretmenleri ile güzel anılar biriktirdi. Türkan Saylan, okul arkadaşlarına çok önem verirdi, onlarla iletişimi yaşama veda edene kadar sürdü.

Üniversite eğitimine, İstanbul Tıp Fakültesi’nde başladı. Öğrenci iken evlendi, anne oldu, sağlık sorunları yaşadı ama hekim olmaktan vazgeçmedi. Mezuniyet sonrasında, Nişantaşı Sosyal Sigortalar Kurumu Cildiye Servisinde asistanlığa başladı. Asistanlık, sadece cilt hastalıklarını öğrenmek demek değildi, aynı zamanda, hastaların sosyal sorunlarını öğrenmek, çözümler üretmek, kliniği yönetmek idi.

Deri ve zührevi hastalıklar uzmanı oldu. Bursa’ya tayin olmuştu ama, İstanbul Tıp Fakültesi Dermatoloji Anabilim Dalı Başkanı Prof. Dr. Osman Yemni’den ‘Başasistanlık’ teklifi aldı ve 1968 yılında bu göreve başladı. 1971’de İngiliz Kültür Heyeti’nin bursuyla İngiltere'de ileri eğitim gördü. 1972 yılında doçent unvanını aldı.1974'te Fransa’da ve 1976’da İngiltere’de kısa süreli çalışmalar yaptı. Cüzzam hastalığı konusunda kendini geliştirdi. 1976 yılında, Cüzzamla Savaş Derneği’ni kurdu, cüzzam hastalarının tedavi ve sosyal sorunlarına çözümler bulmaya başladı.

Başasistan olarak girdiği dermatoloji kliniğinin kapısından, 2002 yılında emekli olarak çıktı. Yıllar içinde, birçok hasta tedavi etti, sosyal sorunları ile ilgilendi, tıp öğrencilerine ders verdi, onları tanımaya anlamaya çalıştı, çok sayıda asistan yetiştirdi, zaman zaman kliniğin yöneticiliğini yaptı, birçok proje üretti, çalıştı ama hep çalıştı. Çalışmak ona şifa idi, zamanı iyi yönetiyor, kısa zaman içinde çok iş üretiyor, kendine iyi gelecek sosyal ve sanat etkinliklerini de hiç kaçırmıyordu.

Kurucularından biri ve Genel Başkanı olduğu Çağdaş Yaşamı Destekleme Derneği(ÇYDD)’nde, 19 yıl boyunca başta kız çocuklarının eğitimi olmak üzere, okumak isteyen ama ekonomik koşulları yetersiz olan binlerce çocuğa eğitim yolunu açtı. Onların, okuyup meslek sahibi olmasını, kendi ayakları üzerinde durmasını sağladı.
Hekim, Hekimlik Sanatı ve Ustalara Saygı
Authors: YILDIZ Pinar , DÖNDERİCİ Ömer
Published: December 2023

Türk tıbbına hizmet etmiş saygıyla anılmayı hak edenlere ayrılmış bir yayında, anılmayı fazlasıyla hak eden ama ünlü olamayanların da hatırlanması gerektiğini düşünüyoruz. Dr Yavuz Erkoçak’ı Türk tıbbını sırtlayarak saygın bir konuma taşıyan, işini en iyi şekilde yapmaya çalışan, tüketim toplumu değerlerine yenik düşmemiş, yüreği ülke ve insan sevgisiyle dolu, ‘vida sıkmakla yetinmeyen’ bilge/ârif yüzlerce hekimin adına selamlıyoruz.
Aziz Sancar
Authors: ULUPİNAR Emel
Published: December 2023

Bu derlemede halen North Carolina Üniversitesi Tıp Fakültesi, Chapel Hill'de akademik çalışmalarına devam etmekte olan Prof.Dr. Aziz Sancar'in otobiyografisi ve akademik çalışmaları yer almaktadır.
THE EFFECT OF MAULI BANANA (Musa acuminata) STEMS AND BASIL LEAVES (Ocimum basilicum) EXTRACTS ON GIC DISCOLORATION
Authors: Norhayati Norhayati , Diana Sherli , Adhani Rosihan
Published: December 2023

Background: The water settable GIC material is easily influenced by fluids and is hydrophilic which causes discoloration of the restoration. Chlorhexidine is the gold standard mouthwash and if used for a long period of time it can cause discoloration of the restoration because it contains chemicals, so an alternative mouthwash made from natural ingredients is needed. A mixture of mauli banana stem and basil leaves extract has a more optimal antibacterial ability. Objective: In general, this study aimed to analyze the effect of a mixture of Mauli banana stem (Musa acuminata) and basil leaves (Ocimum basilicum) extracts on discoloration of the water settable GIC. Methods: This research is a true experimental research with pre test and post test designs with control group design. This study was divided into three treatments, namely water settable GIC soaked in a mixture of extracts of banana stems and basil leaves at concentrations of 75%, 100%, and 0.2% chlorhexidine gluconate (positive control). Color measurement was carried out using a series of digital analysis tools consisting of a Samsung A1 macro camera, Peyond series PD-480 SL and the MATLAB application. Results: The results of the Kruskal-Wallis statistical test showed p value = 0.282 (> 0.05), meaning that there was no significant difference in color change between the three treatments. Conclusion: There is an effect of a mixture banana mauli stems (Musa acuminata) and basil leaves (Ocimum basilicum) extracts at concentrations of 75%, 100%, and 0.2% chlorhexidine gluconate on the discoloration of the water settable GIC. Keywords: Basil leaves (Ocimum basilicum), CIELAB system, discoloration, mauli banana stem (Musa acuminata), water settable GIC