bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of the Poor Oral Health Status of Children on Their Families: An Analytical Cross-Sectional Study
Authors: Quadri Mir Faeq Ali , Jaafari Fatimah Rasheed M. , Mathmi Noha Ahmed A. , Huraysi Nouf Hassan F. , Nayeem Maryam , Jessani Abbas , Tadakamadla Santosh Kumar , Tadakamadla Jyothi
Published: July 2021

The impact of poor oral health may not just be limited to the children themselves but can impact their families. The current study aims to perform psychometric analyses of the Arabic version of the Family Impact Scale and investigate the association of its domains with the oral health status of children. This cross-sectional study was carried out in a sample of 500 parent-child dyads from high schools of Jazan city of the Kingdom of Saudi Arabia. The Arabic version of the Family Impact Scale was subjected to reliability and validity tests. The explanatory variables in the current study are: the oral health status, parents combined income, parents’ education, age and sex of the child. The descriptive analysis was reported using proportions, this was followed by the bivariate and multivariable analyses. About 24.2% of children were reported to have fair, poor, and very poor oral health. A lower frequency of family impact corresponded with better oral health (OH) status of children (p < 0.001). The likelihood of parent’s taking time off from work and having financial difficulties was nearly two-times greater if their children had poor oral health. Similarly, interruption in sleep and other normal activities of parents is four times and five times greater, respectively, if the child has poor oral health status. Thus, the poor oral health of school children in the Jazan region of Saudi Arabia is a matter of grave concern as it is observed to be associated with family impacts; particularly affecting the parent’s work, sleep, and other normal family activities.
A Pilot Clinical Study of Hyperacute Serum Treatment in Osteoarthritic Knee Joint: Cytokine Changes and Clinical Effects
Authors: Olmos Calvo Isabel , Fodor Eszter , Kardos Dorottya , Hornyák István , Hinsenkamp Adél , Kuten-Pella Olga , Gyevnár Zsuzsanna , Erdélyi Gábor , Bárdos Tamás , Paukovits Tamás Mirkó , Magos Krisztián , Béres György , Nehrer Stefan , Lacza Zsombor
Published: July 2021

The serum fraction of platelet-rich fibrin (hyperacute serum) has been shown to improve cartilage cell proliferation in in vitro osteoarthritic knee joint models. We hypothesize that hyperacute serum may be a potential regenerative therapeutic for osteoarthritic knees. In this study, the cytokine milieu at the synovial fluid of osteoarthritic knee joints exposed to hyperacute serum intraarticular injections was investigated. Patients with knee osteoarthritis received three injections of autologous hyperacute serum; synovial fluid was harvested before each injection and clinical monitoring was followed-up for 6 months. Forty osteoarthritic-related cytokines, growth factors and structural proteins from synovial fluid were quantified and analysed by Multivariate Factor Analysis. Hyperacute serum provided symptomatic relief regarding pain and joint stability for OA patients. Both patients “with” and “without effusion knees” had improved VAS, KOOS and Lysholm-Tegner scores 6 months after of hyperacute serum treatment. Synovial fluid analysis revealed two main clusters of proteins reacting together as a group, showing strong and significant correlations with their fluctuation patterns after hyperacute serum treatment. In conclusion, hyperacute serum has a positive effect in alleviating symptoms of osteoarthritic knees. Moreover, identified protein clusters may allow the prediction of protein expression, reducing the number of investigated proteins in future studies.
Axial Error of Spindle Measurements Using a High-Frequency-Modulated Interferometer
Authors: Nguyen Thanh-Trung , Vu Thanh-Tung , Nguyen Thanh-Dong , Vu Toan-Thang
Published: July 2021

In this paper, a novel, compact, and high-precision axial error measurement using a frequency-modulated interferometer is developed. Normally, heterodyne interferometers are a powerful system for small displacement measurements due to their property of being less sensitive to temperature and pressure variations. However, the maximum measurement speed of the heterodyne interferometer is around 5 m/s because it is usually limited by the difference in frequency between the two components of the laser beam, which is no larger than 3 MHz or 20 MHz corresponding laser source based on the Zeeman effect and acousto-optic modulator, respectively. The proposed measuring system is realized by modulating the frequency of the laser diode source at a high modulation frequency and using lock-in amplifiers to extract the harmonics of the interference signal. The measurement speed is proportional to the modulation frequency. Thus, the higher the modulation frequency, the higher the measuring speed attains. The frequency-modulated interferometer is then applied to measure the axial error of an ultra-precision spindle. The proposed system can be a capable solution for noncontact and high-precision spindle error measurements in the machining process.
Effect of Sulfurization Time on the Physical Properties of Tin (II) Monosulfide Thin Films
Authors: Minnam Reddy Vasudeva Reddy , Gedi Sreedevi , Amate Rutuja U. , Ramakrishna Reddy K. T. , Kim Woo Kyoung , Park Chinho
Published: July 2021

Tin (II) monosulfide (SnS) films were prepared via sulfurization using sputtered Sn precursors of the tin metal layers in the presence of elemental sulfur vapor as a function of sulfurization time (ts) in the range of 30–180 min while keeping other parameters constant. The properties of these sulfurized layers were examined through suitable characterization techniques. The diffraction patterns exhibited various planes with the orientations (110), (120), (021), (101), (111), (211), (131), (210), (141), (002), (112), (122), and (042) corresponding to orthorhombic SnS at ts ≤ 90 min. However, for ts ≥ 120 min, the diffraction patterns showed a single (111) plane and enhanced the intensity of the peak with the increase of ts up to 150 min; with further increase of time, the peak intensity was found to decrease. The Raman spectra of films sulfurized at various ts showed modes at 95, 162, 189, 219, and 284 cm−1 for times were lower than 120 min and 95, 189, and 219 cm−1 for ts ≥ 120 min, related to SnS. In the transmittance spectra of the sulfurized films, it is clear that the film grown at ts = 30 min had higher transmittance, and with the increase of ts to 120 min, transmittance was decreased. For further extension of ts to 150 min, a sharp falling of the absorption edge was observed.
Seasonal and Interspecific Variation in Frugivory by a Mixed Resident-Migrant Overwintering Songbird Community
Authors: Carter Wales A. , Pearson Scott F. , Smith Adam D. , McWilliams Scott R. , Levey Douglas J.
Published: July 2021

Many temperate passerine bird species switch from diets of mostly invertebrates in the spring and summer to diets that include fruit and seeds in the fall and winter. However, relatively few studies have quantified diet composition or the extent of seasonal shifts during the non-breeding period, particularly among species and across communities with both residents and migrants. We measured carbon and nitrogen stable isotope values in food items (fruits, C3 and C4 seeds, and insects from various trophic levels and plant communities) and in multiple tissues (feathers and plasma/whole blood) from 11 species of songbirds wintering in the southeastern U.S. We combined these diet and tissue values with empirically derived discrimination factors and used concentration-dependent mixing models to quantify seasonal diet shifts. We also validated mixing model results with data from fecal samples. Diets in this bird community, as delineated N and C isotopic space, diverged in the fall and winter relative to the summer as consumption of fruits and seeds increased. Across this songbird community, estimated contributions of fruit to plasma/whole blood increased from 16.2 ± 7.5% in the fall (mean ± SD; range: 4–26%) to 21.7 ± 10.3% (range: 9–37%) in the winter, while contributions of seeds increased from 29.4 ± 2.6% (range: 28–32%) in the fall to 36.6 ± 4.8% (range: 32–42%) in the winter. Fecal data showed qualitatively similar trends to mixing models, but consistently estimated higher contributions of fruit. Our work indicates that fruits and seeds constitute substantial sources of sustenance for non-breeding songbirds, there is considerable separation of resource use among species in the fall and winter, and fecal estimates of contributions to songbird tissues should be interpreted cautiously.
Contemporary Teacher Education: A Global Perspective-Introduction to a Special Collection of Research
Authors: Tirri Kirsi
Published: July 2021

This special issue on “Contemporary Teacher Education: A Global Perspective” contains eleven articles focused on varied current topics in teacher education all over the world [...]
A Novel Anomaly Behavior Detection Scheme for Mobile Ad Hoc Networks
Authors: Chugh Neeraj , Tomar Geetam Singh , Bhadoria Robin Singh , Saxena Neetesh
Published: July 2021

To sustain the security services in a Mobile Ad Hoc Networks (MANET), applications in terms of confidentially, authentication, integrity, authorization, key management, and abnormal behavior detection/anomaly detection are significant. The implementation of a sophisticated security mechanism requires a large number of network resources that degrade network performance. In addition, routing protocols designed for MANETs should be energy efficient in order to maximize network performance. In line with this view, this work proposes a new hybrid method called the data-driven zone-based routing protocol (DD-ZRP) for resource-constrained MANETs that incorporate anomaly detection schemes for security and energy awareness using Network Simulator 3. Most of the existing schemes use constant threshold values, which leads to false positive issues in the network. DD-ZRP uses a dynamic threshold to detect anomalies in MANETs. The simulation results show an improved detection ratio and performance for DD-ZRP over existing schemes; the method is substantially better than the prevailing protocols with respect to anomaly detection for security enhancement, energy efficiency, and optimization of available resources.
Stability Improvement of Dynamic EV Wireless Charging System with Receiver-Side Control Considering Coupling Disturbance
Authors: Chen Kaiwen , Cheng Ka Wai Eric , Yang Yun , Pan Jianfei
Published: July 2021

Receiver-side control has been a reliable practice for regulating the transferred energy to the batteries in the electric vehicle (EV) wireless power transfer (WPT) systems. Nonetheless, the unpredictable fluctuation of the mutual inductance in dynamic wireless charging brings extreme instability to the charging process. This overshoot that appears in instant vibrations may largely increase the voltage/current stress of the system, and even cause catastrophic failure in the battery load. In addition, the speed of the vehicles may lead to untraceable steady-state operation. However, existing solutions to the above two issues suffer from either long communication time delay or significantly compromised output regulation. In this paper, the slow dynamics and the overshoot issues of the WPT system are elaborated in theory, and the small-signal model mainly considering mutual inductance disturbance is established. A simple feedforward control is proposed for overshoot damping and fast system dynamics. Experimental results validate that the overshoot can be reduced by 13% and the settling time is improved by 50% in vehicle braking or acceleration. In constant speed driving, the battery charging ripple is decreased by 12% and ensures better system stability.
Fabrication and Performance Evaluation of Cold Thermal Energy Storage Tanks Operating in Water Chiller Air Conditioning System
Authors: Nguyen Xuan Vien
Published: July 2021

In this study, cold and thermal storage systems were designed and manufactured to operate in combination with the water chiller air-conditioning system of 105.5 kW capacity, with the aim of reducing operating costs and maximizing energy efficiency. The cold storage tank used a mixture of water and 10 wt.% glycerin as a phase-change material (PCM), while water was used as heat transfer fluid (HTF). The cold storage heat exchanger was made of polyvinyl chloride (PVC). On the other hand, the thermal storage tank used water as the storage fluid with a capacity of 50 L of hot water per hour. The thermal storage did not use a pump for water transfer through the heat exchanger, so as to save energy and operating costs. In this paper, the operating parameters of the cold and thermal storage tanks are shown according to the results of experimental research, including the temperatures of cooling and heating load, heat transfer fluid, and cold storage material during the discharge process, as well as the discharge duration. The system assisted the air conditioner in cooling the internship workshop space at the university with an area of 400 m2, contributing to a remarkable reduction in air-conditioning system operating costs during the daytime. Furthermore, the system recovered waste heat from the compressor of the water chiller, and a thermal storage system was successfully built and operated, providing 50 L of hot water at a temperature of 60 °C per hour to serve the everyday needs of school students. This design was suitable for the joint operation of cold and thermal storage tanks and the water chiller air-conditioning system for cooling and heating applications.
Bioaccessibility and Bioactivity of Cereal Polyphenols: A Review
Authors: Ed Nignpense Borkwei , Francis Nidhish , Blanchard Christopher , Santhakumar Abishek Bommannan
Published: July 2021

Cereal bioactive compounds, especially polyphenols, are known to possess a wide range of disease preventive properties that are attributed to their antioxidant and anti-inflammatory activity. However, due to their low plasma concentrations after oral intake, there is controversy regarding their therapeutic benefits in vivo. Within the gastrointestinal tract, some cereal polyphenols are absorbed in the small intestine, with the majority accumulating and metabolised by the colonic microbiota. Chemical and enzymatic processes occurring during gastrointestinal digestion modulate the bioactivity and bioaccessibility of phenolic compounds. The interactions between the cereal polyphenols and the intestinal epithelium allow the modulation of intestinal barrier function through antioxidant, anti-inflammatory activity and mucin production thereby improving intestinal health. The intestinal microbiota is believed to have a reciprocal interaction with polyphenols, wherein the microbiome produces bioactive and bioaccessible phenolic metabolites and the phenolic compound, in turn, modifies the microbiome composition favourably. Thus, the microbiome presents a key link between polyphenol consumption and the health benefits observed in metabolic conditions in numerous studies. This review will explore the therapeutic value of cereal polyphenols in conjunction with their bioaccessibility, impact on intestinal barrier function and interaction with the microbiome coupled with plasma anti-inflammatory effects.
Effect of Quinoa (Chenopodium quinoa Willd.) Starch and Seeds on the Physicochemical and Textural and Sensory Properties of Chicken Meatballs during Frozen Storage
Authors: Park Jin-Hwa , Lee Yun-Jin , Lim Jeong-Gyu , Jeon Ji-Hye , Yoon Ki-Sun
Published: July 2021

The effects of starch (corn and quinoa) and quinoa seeds on chicken meatballs’ physicochemical, textural, and sensory properties were investigated during frozen storage. The chicken meatballs were prepared with corn starch (CS), quinoa starch (QS), quinoa seeds (Q), and combinations of corn starch and quinoa seeds (CS-Q), and quinoa starch and quinoa seeds (QS-Q), which were subjected to five freeze–thaw (F-T) cycles of temperature fluctuation conditions during frozen storage. Regardless of the type used (CS or QS), adding starch resulted in fewer cooking, drip, and reheating losses in chicken meatballs during frozen storage. The values of the hardness, gumminess, and chewiness of chicken meatballs with CS or QS were half those of chicken meatballs without starch, indicating that the addition of starch inhibited the change in the meatballs’ texture. The total volatile basic nitrogen (TVB-N) and thiobarbituric acid reactive substance (TBARS) values were progressive but did not dynamically increase during five F-T cycles. Chicken meatballs containing CS-Q or QS-Q showed significantly lower TBARS values than those with CS, QS, or Q after five F-T cycles. Adding quinoa seeds significantly increased the antioxidant activity and the chewiness of meatballs (p < 0.05) compared with starch only. The addition of the combination of QS-Q to chicken meatballs increased the values of taste, texture, and overall acceptability, indicating that quinoa starch and seeds may be introduced as premium ingredients to frozen meat products.
Contribution of Multiple Inherited Variants to Autism Spectrum Disorder (ASD) in a Family with 3 Affected Siblings
Authors: Dhaliwal Jasleen , Qiao Ying , Calli Kristina , Martell Sally , Race Simone , Chijiwa Chieko , Glodjo Armansa , Jones Steven , Rajcan-Separovic Evica , Scherer Stephen W. , Lewis Suzanne
Published: July 2021

Autism Spectrum Disorder (ASD) is the most common neurodevelopmental disorder in children and shows high heritability. However, how inherited variants contribute to ASD in multiplex families remains unclear. Using whole-genome sequencing (WGS) in a family with three affected children, we identified multiple inherited DNA variants in ASD-associated genes and pathways (RELN, SHANK2, DLG1, SCN10A, KMT2C and ASH1L). All are shared among the three children, except ASH1L, which is only present in the most severely affected child. The compound heterozygous variants in RELN, and the maternally inherited variant in SHANK2, are considered to be major risk factors for ASD in this family. Both genes are involved in neuron activities, including synaptic functions and the GABAergic neurotransmission system, which are highly associated with ASD pathogenesis. DLG1 is also involved in synapse functions, and KMT2C and ASH1L are involved in chromatin organization. Our data suggest that multiple inherited rare variants, each with a subthreshold and/or variable effect, may converge to certain pathways and contribute quantitatively and additively, or alternatively act via a 2nd-hit or multiple-hits to render pathogenicity of ASD in this family. Additionally, this multiple-hits model further supports the quantitative trait hypothesis of a complex genetic, multifactorial etiology for the development of ASDs.
Angiotensin-Converting Enzyme 2 (ACE2) as a Potential Diagnostic and Prognostic Biomarker for Chronic Inflammatory Lung Diseases
Authors: Marčetić Dejan , Samaržija Miroslav , Vukić Dugac Andrea , Knežević Jelena
Published: July 2021

Chronic inflammatory lung diseases are characterized by uncontrolled immune response in the airways as their main pathophysiological manifestation. The lack of specific diagnostic and therapeutic biomarkers for many pulmonary diseases represents a major challenge for pulmonologists. The majority of the currently approved therapeutic approaches are focused on achieving disease remission, although there is no guarantee of complete recovery. It is known that angiotensin-converting enzyme 2 (ACE2), an important counter-regulatory component of the renin–angiotensin–aldosterone system (RAAS), is expressed in the airways. It has been shown that ACE2 plays a role in systemic regulation of the cardiovascular and renal systems, lungs and liver by acting on blood pressure, electrolyte balance control mechanisms and inflammation. Its protective role in the lungs has also been presented, but the exact pathophysiological mechanism of action is still elusive. The aim of this study is to review and discuss recent findings about ACE2, including its potential role in the pathophysiology of chronic inflammatory lung diseases:, i.e., chronic obstructive pulmonary disease, asthma, and pulmonary hypertension. Additionally, in the light of the coronavirus 2019 disease (COVID-19), we will discuss the role of ACE2 in the pathophysiology of this disease, mainly represented by different grades of pulmonary problems. We believe that these insights will open up new perspectives for the future use of ACE2 as a potential biomarker for early diagnosis and monitoring of chronic inflammatory lung diseases.
Survey on Sternal Wound Management in the Italian Pediatric Cardiac Intensive Care Units
Authors: Prendin Angela , Tabacco Benedicta , Fazio Paola Claudia , Strini Veronica , Brugnaro Luca , De Barbieri Ilaria
Published: July 2021

(1) Background: a review of the literature found a lack of standardized pediatric guidelines regarding wound management after cardiac surgery. (2) Objective: the aim of the study is to investigate the cardiac surgical wound management in Italian pediatric cardiac intensive care units. (3) Methods: we sent an online questionnaire to the 13 Italian pediatric cardiac intensive care units. (4) Results: ten pediatric cardiac intensive care units (77%) have a protocol for the management of the cardiac surgical wound. The staff members that mainly have the responsibility for the wound management after cardiac surgery are registered nurses and physicians together both in the pediatric cardiac intensive care units (69%), and when a patient is transferred to another ward (62%). Thirty-eight percent of the pediatric cardiac intensive care units have a protocol used to monitor wound infection, and the staff mostly uses a written shift report (54%) to monitor the infection. (5) Discussion: this is the first survey to investigate the management of the wound after cardiac surgery in Italian pediatric cardiac intensive care units. The small sample size and the fact that the centers involved are only Italian cardiac intensive care units are the limits of this study. (6) Conclusions: in the Italian pediatric cardiac intensive care units it emerged that there is a diversity in the treatments adopted and a lack of specific protocols in the management of the pediatric cardiac surgical wound.
“Industrious Revolution” Revisited: A Variety of Diligence Derived from a Long-Term Local History of Kuta in Kyô-Otagi, a Former County in Japan
Authors: Murayama Satoshi , Nakamura Hiroko
Published: July 2021

Jan de Vries revised Akira Hayami’s original theory of the “Industrious Revolution” to make the idea more applicable to early modern commercialization in Europe, showcasing the development of the rural proletariat and especially the consumer revolution and women’s emancipation on the way toward an “Industrial Revolution.” However, Japanese villages followed a different path from the Western trajectory of the “Industrious Revolution,” which is recognized as the first step to industrialization. This article will explore how a different form of “industriousness” developed in Japan, covering medieval, early modern, and modern times. It will first describe why the communal village system was established in Japan and how this unique institution, the self-reliance system of a village, affected commercialization and industrialization and was sustained until modern times. Then, the local history of Kuta Village in Kyô-Otagi, a former county located close to Kyoto, is considered over the long term, from medieval through modern times. Kuta was not directly affected by the siting of new industrial production bases and the changes brought to villages located nearer to Kyoto. A variety of diligent interactions with living spaces is introduced to demonstrate that the industriousness of local women was characterized by conscience-driven perseverance.
Radiological Dose Assessment to Members of the Public Using Consumer Products Containing Naturally Occurring Radioactive Materials in Korea
Authors: Joseph Soja Reuben , Kim Juyoul
Published: July 2021

Various products containing a small number of added radionuclides are commonly available for use worldwide. However, frequent use of such products puts the public at risk of radiation exposure. In this study, dose assessments to members of the public using consumer products containing naturally occurring radioactive materials (NORMs) were conducted for various usage scenarios to evaluate the external and internal exposure dose. Data for this study were obtained from previous literature and were statistically analyzed using Boxplot to determine the input data for assessment. A normalized value of activity concentration was used for dose evaluation. In addition to other external and internal dose calculation codes, analytical calculations were used to perform age-dependent. Based on analytical calculations, the highest total effective dose equivalent (TEDE) received from necklace products at the upper whiskers with an activity concentration of 4.21 Bq/g for 238U, 24.4 Bq/g for 232Th, and 0.55 Bq/g for 40K for various age groups is 2.03 mSv/y for 1 year old, 1.24 mSv/y for 10 years old and 1.11 mSv/y for adult, which are above the international commission for radiation protection (ICRP) recommended public dose limit of 1 mSv/y. Results of external and internal exposure dose obtained using Microshield code, IMBA code and Visual Monte Carlo (VMC) code are all below the recommended public dose limit of 1 mSv/y.
Development of a Mobile Application of Internet-Based Support Program on Parenting Outcomes for Primiparous Women
Authors: Li Xilin , Zhang Yao , Ye Ziwen , Huang Lingling , Zheng Xujuan
Published: July 2021

Primiparous women usually experience various parenting problems after childbirth that have negative effects on the well-being of mothers and infants. Although e-Support technology could provide an innovative and easily accessible intervention approach, mobile-phone interventions remain limited for Chinese primiparous women. Therefore, a new mobile application (APP) called the “Internet-based Support Program” (“ISP”) was designed, incorporating the self-efficacy theory and the social-exchange theory for Chinese first-time mothers to improve their levels of maternal self-efficacy (MSE), social support, and satisfaction, as well as to reduce their postpartum depression symptoms. The research was conducted to develop and optimize the “ISP” APP for new mothers via a theory-, evidence-, and person-based approach. Five modules of “learning forum”, “communication forum”, “ask-the-expert forum”, “baby home forum”, and “reminder forum” were included in the APP to meet various parenting needs of first-time mothers; and its contents and functions were validated by the experts and primiparous women. The majority of participants gave positive feedback on the APP’s perceived ease of use and usefulness. The “ISP” APP was the first designed for Chinese primiparous women, and a multicenter randomized controlled trial (RCT) will be conducted to measure its effectiveness on parenting outcomes.
The Supply of Calories, Proteins, and Fats in Low-Income Countries: A Four-Decade Retrospective Study
Authors: Erokhin Vasilii , Diao Li , Gao Tianming , Andrei Jean-Vasile , Ivolga Anna , Zong Yuhang
Published: July 2021

Over the past decades, both the quantity and quality of food supply for millions of people have improved substantially in the course of economic growth across the developing world. However, the number of undernourished people has resumed growth in the 2010s amid food supply disruptions, economic slowdowns, and protectionist restrictions to agricultural trade. Having been common to most nations, these challenges to the food security status of the population still vary depending on the level of economic development and national income of individual countries. In order to explore the long-run determinants of food supply transformations, this study employs five-stage multiple regression analysis to identify the strengths and directions of effects of agricultural production parameters, income level, price indices, food trade, and currency exchange on supply of calories, proteins, and fats across 11 groups of agricultural products in 1980–2018. To address the diversity of effects across developing nations, the study includes 99 countries of Asia, Europe, Latin America, the Middle East, and Africa categorized as low-income, lower-middle-income, and upper-middle-income economies. It is found that in low-income countries, food supply parameters are more strongly affected by production factors compared to economic and trade variables. The effect of economic factors on the food supply of higher-value food products, such as meat and dairy products, fruit, and vegetables, increases with the rise in the level of income, but it stays marginal for staples in all three groups of countries. The influence of trade factors on food supply is stronger compared to production and economic parameters in import-dependent economies irrelevant of the gross national income per capita. The approach presented in this paper contributes to the research on how food supply patterns and their determinants evolve in the course of economic transformations in low-income countries.
Understanding the Implementation of “Sit Less at Work” Interventions in Three Organisations: A Mixed Methods Process Evaluation
Authors: Mackenzie Kelly , Such Elizabeth , Norman Paul , Goyder Elizabeth
Published: July 2021

Long periods of workplace sitting are associated with poor health outcomes. Interventions to reduce workplace sitting time have had variable impacts, the reasons for which require further investigation. In this paper, we report on a process evaluation aiming to determine the intervention fidelity of three “sit less at work” interventions and to explore barriers and enablers to implementation, using a mixed methods “before and after” intervention study design. Convenience samples of staff were recruited from three diverse organisations to participate in pre- and post-intervention online questionnaires, objective measures of sitting time (using activPAL3™ devices) and post-intervention focus groups. Intervention implementers and key personnel were also recruited to participate in post-intervention focus groups and interviews. The process evaluation found that none of the interventions were implemented as intended, with no consistent reductions in sitting time. Contextual and organisational cultural barriers included workload pressures and the social norms of sitting, competing priorities, lack of management buy-in, and perceptions of where the responsibility for behaviour change should come from. To ensure effective implementation of future initiatives, deeper organisational-level change, requiring buy-in from all levels of management and staff, may be needed to shift organisational culture and associated social norms.
Factors Associated with Physical Activity among People with Hypertension in a Rural Area in Bangladesh: Baseline Data from a Cluster Randomized Control Trial
Authors: Islam Fakir M Amirul
Published: July 2021

The health benefits of physical activity (PA) are well recognized, and PA levels vary in different populations. The study aimed to investigate PA levels and associated sociodemographic factors among people with hypertension in a rural area in Bangladesh. Baseline data were part of a cluster randomized controlled trial of 307 adults aged 30–75 years to study the effectiveness of PA and lifestyle changes in lowering blood pressure. The outcome variables were PA at work, commuter, recreation, metabolic equivalent task (MET)-minute per week and sitting time. Total 68 (22.1%) people participated in vigorous-intensity activity, 23 (7.5%) participated in moderate-intensity sports. Overall, 83% of people were physically active more than 600 MET-min. Women (OR 2.95, 95% CI, 1.36–6.39) compared to men, and people with no education (OR 4.47, 95% CI, 1.62–12.33) compared to people with secondary school certificates or above were less physically active. Of total PA, 63% were work-related, and 1% were recreation-related for women, and these figures were 55% and 3% for men. The study reports that vigorous-intensity PA is low, and recreation time is minimal. Routine PA, especially for women and people with low education levels, should be encouraged to increase PA to manage hypertension.
Prevalence of Larval Breeding Sites and Seasonal Variations of Aedes aegypti Mosquitoes (Diptera: Culicidae) in Makkah Al-Mokarramah, Saudi Arabia
Authors: Ali Elfadol Obeid Mohamed , Babalghith Ahmed Omer , Bahathig Adil Omer Saeed , Toulah Fawzia Hassan Suleiman , Bafaraj Turki Ghazi , Al-Mahmoudi Sameer Mohammed Yousef , Alhazmi Abdullah Mousa Fawaz , Abdel-Latif Mohamed E.
Published: July 2021

Since 1994, dengue fever (DF) transmission rates have increased significantly in Saudi Arabia (KSA). Climatic, geographic, and demographic conditions make KSA especially suitable for DF’s spread. Still, there are insufficient strategies for controlling the Aedes species that transmit DF virus (DENV). To develop effective management strategies, it is necessary to identify Aedes species and the ecological habitat of larvae in Makkah Al-Mokarramah, KSA. We conducted a longitudinal survey of Aedes mosquitoes in 14 localities from January 2015 to December 2015. World Health Organization (WHO) inspection kits for larvae were used to detect and sample larvae, along with pictorial keys. A total of 42,981 potential Aedes larval breeding sites were surveyed. A total of 5403 (12.6%) sites had at least one water source positive for Aedes aegypti (Linnaeus) mosquitoes. Among the total of 15,133 water sources surveyed within the sampled sites, 1815 (12.0%) were positive for Aedes aegypti. Aedes aegypti was the only Aedes species identified in the course of the survey. The presence of such a large immature population may indicate an imminent outbreak of DF in the near future unless proper implementation of control and elimination of Aedes aegypti are undertaken. Additionally, the adaptation of Aedes aegypti to the arid climate of Makkah needs further investigation.
Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model
Authors: Tai Chien-Cheng , Lo Hon-Lok , Liaw Chen-Kun , Huang Yu-Min , Huang Yen-Hua , Yang Kuo-Yi , Huang Chih-Chieh , Huang Shin-I , Shen Hsin-Hsin , Lin Tzu-Hung , Lu Chun-Kuan , Liu Wen-Chih , Sun Jui-Sheng , Tsai Pei-I , Chen Chih-Yu
Published: July 2021

This study evaluated the biocompatibility and biological performance of novel additive-manufactured bioabsorbable iron-based porous suture anchors (iron_SAs). Two types of bioabsorbable iron_SAs, with double- and triple-helical structures (iron_SA_2_helix and iron_SA_3_helix, respectively), were compared with the synthetic polymer-based bioabsorbable suture anchor (polymer_SAs). An in vitro mechanical test, MTT assay, and scanning electron microscope (SEM) analysis were performed. An in vivo animal study was also performed. The three types of suture anchors were randomly implanted in the outer cortex of the lateral femoral condyle. The ultimate in vitro pullout strength of the iron_SA_3_helix group was significantly higher than the iron_SA_2_helix and polymer_SA groups. The MTT assay findings demonstrated no significant cytotoxicity, and the SEM analysis showed cells attachment on implant surface. The ultimate failure load of the iron_SA_3_helix group was significantly higher than that of the polymer_SA group. The micro-CT analysis indicated the iron_SA_3_helix group showed a higher bone volume fraction (BV/TV) after surgery. Moreover, both iron SAs underwent degradation with time. Iron_SAs with triple-helical threads and a porous structure demonstrated better mechanical strength and high biocompatibility after short-term implantation. The combined advantages of the mechanical superiority of the iron metal and the possibility of absorption after implantation make the iron_SA a suitable candidate for further development.
Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties
Authors: Jabłońska Joanna , Onyszko Magdalena , Konopacki Maciej , Augustyniak Adrian , Rakoczy Rafał , Mijowska Ewa
Published: July 2021

Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity. We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles. The proposed processing of bacterial cellulose resulted in the formation of nanofibers. Antimicrobial behavior was tested using E. coli ATCC® 25922™ following the ASTM E2149-13a standard. The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard. The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties. Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
Peripheral Opioid Receptor Blockade Enhances Epithelial Damage in Piroxicam-Accelerated Colitis in IL-10-Deficient Mice
Authors: Mas-Orea Xavier , Sebert Morgane , Benamar Mehdi , Petitfils Camille , Blanpied Catherine , Saoudi Abdelhadi , Deraison Céline , Barreau Frederick , Cenac Nicolas , Dietrich Gilles
Published: July 2021

Mucosal CD4+ T lymphocytes display a potent opioid-mediated analgesic activity in interleukin (IL)-10 knockout mouse model of inflammatory bowel diseases (IBD). Considering that endogenous opioids may also exhibit anti-inflammatory activities in the periphery, we examined the consequences of a peripheral opioid receptor blockade by naloxone-methiodide, a general opioid receptor antagonist unable to cross the blood–brain barrier, on the development of piroxicam-accelerated colitis in IL-10-deficient (IL-10-/-) mice. Here, we show that IL-10-deficient mice treated with piroxicam exhibited significant alterations of the intestinal barrier function, including permeability, inflammation-related bioactive lipid mediators, and mucosal CD4+ T lymphocyte subsets. Opioid receptor antagonization in the periphery had virtually no effect on colitis severity but significantly worsened epithelial cell apoptosis and intestinal permeability. Thus, although the endogenous opioid tone is not sufficient to reduce the severity of colitis significantly, it substantially contributes to the protection of the physical integrity of the epithelial barrier.
[68Ga]Ga-DFO-c(RGDyK): Synthesis and Evaluation of Its Potential for Tumor Imaging in Mice
Authors: Krajcovicova Sona , Daniskova Andrea , Bendova Katerina , Novy Zbynek , Soural Miroslav , Petrik Milos
Published: July 2021

Angiogenesis has a pivotal role in tumor growth and the metastatic process. Molecular imaging was shown to be useful for imaging of tumor-induced angiogenesis. A great variety of radiolabeled peptides have been developed to target αvβ3 integrin, a target structure involved in the tumor-induced angiogenic process. The presented study aimed to synthesize deferoxamine (DFO)-based c(RGD) peptide conjugate for radiolabeling with gallium-68 and perform its basic preclinical characterization including testing of its tumor-imaging potential. DFO-c(RGDyK) was labeled with gallium-68 with high radiochemical purity. In vitro characterization including stability, partition coefficient, protein binding determination, tumor cell uptake assays, and ex vivo biodistribution as well as PET/CT imaging was performed. [68Ga]Ga-DFO-c(RGDyK) showed hydrophilic properties, high stability in PBS and human serum, and specific uptake in U-87 MG and M21 tumor cell lines in vitro and in vivo. We have shown here that [68Ga]Ga-DFO-c(RGDyK) can be used for αvβ3 integrin targeting, allowing imaging of tumor-induced angiogenesis by positron emission tomography.
New Partners Identified by Mass Spectrometry Assay Reveal Functions of NCAM2 in Neural Cytoskeleton Organization
Authors: Parcerisas Antoni , Ortega-Gascó Alba , Hernaiz-Llorens Marc , Odena Maria Antonia , Ulloa Fausto , de Oliveira Eliandre , Bosch Miquel , Pujadas Lluís , Soriano Eduardo
Published: July 2021

Neuronal cell adhesion molecule 2 (NCAM2) is a membrane protein with an important role in the morphological development of neurons. In the cortex and the hippocampus, NCAM2 is essential for proper neuronal differentiation, dendritic and axonal outgrowth and synapse formation. However, little is known about NCAM2 functional mechanisms and its interactive partners during brain development. Here we used mass spectrometry to study the molecular interactome of NCAM2 in the second postnatal week of the mouse cerebral cortex. We found that NCAM2 interacts with >100 proteins involved in numerous processes, including neuronal morphogenesis and synaptogenesis. We validated the most relevant interactors, including Neurofilaments (NEFs), Microtubule-associated protein 2 (MAP2), Calcium/calmodulin kinase II alpha (CaMKIIα), Actin and Nogo. An in silico analysis of the cytosolic tail of the NCAM2.1 isoform revealed specific phosphorylation site motifs with a putative affinity for some of these interactors. Our results expand the knowledge of NCAM2 interactome and confirm the key role of NCAM2 in cytoskeleton organization, neuronal morphogenesis and synaptogenesis. These findings are of interest in explaining the phenotypes observed in different pathologies with alterations in the NCAM2 gene.
Function of Polyamines in Regulating Cell Cycle Progression of Cultured Silkworm Cells
Authors: Chang Li , Li Zhiqing , Guo Hao , Zhang Wenchang , Lan Weiqun , Wang Jue , Shen Guanwang , Xia Qingyou , Zhao Ping
Published: July 2021

Background: Putrescine, spermidine, and spermine are polyamines that are ubiquitously distributed in prokaryotic and eukaryotic cells, which play important roles in cell proliferation and differentiation. Methods: We investigated the expression profiles of polyamine pathway genes by qRT-PCR in different tissues of the lepidopteran silkworm. The polyamine levels in cultured silkworm cells were measured by HPLC. Spermidine and polyamine biosynthetic inhibitors were used for treating the cultured silkworm cells in order to clarify their effects on cell cycle progression. Results: We identified the anabolic and catabolic enzymes that are involved in the polyamine biosynthetic pathway in silkworm. Transcriptional expression showed at least seven genes that were expressed in different silkworm tissues. Treatments of the cultured silkworm cells with spermidine or inhibitor mixtures of DFMO and MGBG induced or inhibited the expression of cell cycle-related genes, respectively, and thus led to changed progression of the cell cycle. Conclusions: The present study is the first to identify the polyamine pathway genes and to demonstrate the roles of polyamines on cell cycle progression via regulation of the expression of cell cycle genes in silkworm.
Development of Monitoring and Mating Disruption against the Chilean Leafroller Proeulia auraria (Lepidoptera: Tortricidae) in Orchards
Authors: Flores M. Fernanda , Bergmann Jan , Ballesteros Carolina , Arraztio Diego , Curkovic Tomislav
Published: July 2021

The leafroller Proeulia auraria (Clarke) (Lepidoptera: Tortricidae) is a native, polyphagous, and growing pest of several fruit crops in Chile; it also has quarantine importance to several markets, thus tools for management are needed. Using synthetic pheromone compounds, we conducted field trials to optimize the blend for monitoring, and to determine the activity period of rubber septa aged under field conditions. We concluded that septa loaded with 200 μg of E11-14:OAc + 60 μg E11-14:OH allowed for efficient trap captures for up to 10 weeks. Using this blend, we studied the phenology of adult males in vineyards, apple, and blueberry orchards, identifying two long flight cycles per season, lasting from September to May and suggesting 2–3 generations during the season. No or low adult activity was observed during January and between late May and late August. Furthermore, mating disruption (MD) field trials showed that application of 250 pheromone point sources using the dispenser wax matrix SPLAT (Specialized Pheromone and Lure Application Technology, 10.5% pheromone) with a total of 78 g/ha of the blend described above resulted in trap shutdown immediately after application, and mating disruption >99% in all orchards for at least 5 months. We concluded that MD is feasible for P. auraria, needing now the development of a commercial product and the strategy (and protocols) necessary to control this pest in conventional and organic orchards in Chile. As far as we know, this is the first report on MD development against a South American tortricid pest.
Pilot Study of Acupuncture’s Antispasmodic Effect on Upper Gastrointestinal Tract during Endoscopic Submucosal Dissection for Early Gastric Cancer: Controlled Clinical Trial
Authors: Suzuki Masao , Ishizaki Naoto , Kayo Takumi , Furuta Taiga , Igarashi Ryo , Maki Takumi , Hoshi Koki , Yamabe Akane , Fujisawa Mariko , Funakubo Akira , Mitsuma Tadamichi , Irisawa Atsushi , Shibukawa Goro
Published: July 2021

A prospective study was conducted in patients with early-stage gastric cancer to determine the efficacy and safety of acupuncture stimulation as an antispasmodic compared with conventional medication during the procedure of endoscopic submucosal dissection (ESD) of the upper gastrointestinal tract. This study was a prospective single blinded quasi-randomized controlled trial. Seventy-three patients who were scheduled to undergo ESD for gastric cancer at Aizu Medical Center between 19 February 2016 and 30 June 2016 were assessed for eligibility for the study. Sixty out of 73 patients were included in the study and assigned into two intervention groups: medication group (MG) and acupuncture group (AG). Ease of the procedure was evaluated using modified NIWA classification (MNC) by endoscopist considering the frequency and amplitude of the upper gastrointestinal peristalsis. For the statistical analysis, Mann–Whitney test was used to compare the differences of MNC values (baseline and end of procedure) between two groups. The difference of MNC found in the AG (−2.00 (−3.0 to −2.0)) was significantly greater than that in the MG (−1.00 (−2.0 to −1.0), p < 0.0001, Mann–Whitney test). We consider that acupuncture to the abdomen could be an alternative antispasmodic method during upper gastrointestinal endoscopic procedure.
Pre-Hospital Pain Management in Children with Injuries: A Retrospective Cohort Study
Authors: Holak Ada , Czapla Michał , Zielińska Marzena
Published: July 2021

Background: The all-too-frequent failure to rate pain intensity, resulting in the lack of or inadequacy of pain management, has long ceased to be an exclusive problem of the young patient, becoming a major public health concern. This study aimed to evaluate the methods used for reducing post-traumatic pain in children and the frequency of use of such methods. Additionally, the methods of pain assessment and the frequency of their application in this age group were analysed. Methods: A retrospective analysis of 2452 medical records of emergency medical teams dispatched to injured children aged 0–18 years in the area around Warsaw (Poland). Results: Of all injured children, 1% (20 out of 2432) had their pain intensity rated, and the only tool used for this assessment was the numeric rating scale (NRS). Children with burns most frequently received a single analgesic drug or cooling (56.2%), whereas the least frequently used method was multimodal treatment combining pharmacotherapy and cooling (13.5%). Toddlers constituted the largest percentage of patients who were provided with cooling (12%). Immobilisation was most commonly used in adolescents (29%) and school-age children (n = 186; 24%). Conclusions: Low frequency of pain assessment emphasises the need to provide better training in the use of various pain rating scales and protocols. What is more, non-pharmacological methods (cooling and immobilisation) used for reducing pain in injured children still remain underutilized.