bluuebunny/crossref_metadata_2025 huggingface dataset:
New analytical solutions of fractional (HIV-1 infection of CD4 + T-cells) using two efficient methods
Authors: Alshahrani Maryam , Ouahid Loubna , Abdou M. A. , Kumar Sachin , Shahrani Jameelah S. Al
Published: October 2023

Here, we use a fractional model to perform an analytical investigation of (HIV-1 infection of CD4+ T-cells). This considered model is analyzed by utilizing a new extended sub-equation method as well as a direct algebraic method. The obtained results demonstrate the influence and superiority of the applied schemes over other analytical methodologies. The series of single solutions with a fractional derived in order achieved with these methods can be classified as trigonometric, hyperbolic, rational, and other basic functions. A comparison of analytical method results is discussed. We also offer some figures showing how exact the solutions are obtained using the two new analytical methods.
A dense tolerance to water stress: Coordination between water relations strategy and carbon investment in leaf and stem across six fruit tree species
Authors: Piña Ismael , Garrido Marco , Seguel Oscar , Opazo Ismael , Faúndez Carlos , Verdugo-Vásquez Nicolás , Villalobos-Soublett Emilio
Published: October 2023

The water relation strategy of a species (iso-anisohydric continuum as one of the most widely used definitions) is a key issue in the context of climate change. Given the difficulty of determining water relations strategy, there is a need for simple traits with a solid theoretical basis to estimate it. Among the many possibilities, traits associated with the "fast-slow" plant economics spectrum are particularly interesting. Avocado, Fig, Mandarin, Olive, Pomegranate, and Vine were characterized in terms of stomatal behavior, water potential at the turgor loss point (TLP), and Hydroscape Area, and the association of these metrics with leaf mass per area (LMA) and wood density (WDen) was explored. Our results showed high coordination between LMA and WDen across the six species, and both traits were related to metrics of water relation strategy. Species with less regulation of their water status tended to invest a greater amount of carbon per unit leaf area or unit stem volume with implications over hydraulic efficiency and water stress tolerance. WDen and TLP were the most powerful traits in estimating the water relation strategy for six fruit species. These traits are easy to measure, time-cost efficient, and appear central to coordinating multiple traits and behaviors along the water relations strategies. It is important to improve the understanding of these traits and their intraspecific variability to advance the understanding of how species and cultivars will respond to future scenarios and to design better selection, breeding, and agronomic strategies for climate change adapted agriculture.
The associations of blood volatile organic compounds with sleep health in the American adults: Data from NHANES 2007-2012
Authors: Chen Ming , Tang Mo , He Yi , Pang Ying , Tang Lili
Published: October 2023

Background
Unhealthy sleep health has become a rising public health epidemic, and environmental issues have become a burgeoning orientation to probe into the factors affecting sleep health. Volatile organic compounds (VOCs), common organic pollutants in the air, were connected with some somatic diseases, while whether VOCs exposure or which VOCs lead to sleep abnormality was yet explored so far.
Methods
We analyzed blood content data of five VOCs (LBXVBF, blood bromoform; LBXVBM, blood bromodichloromethane; LBXVCF, blood chloroform; LBXVCM, blood dibromochloromethane; LBXVME, blood MTBE) reported for 5740 adults in NHANES 2007–2012. Sleep health categories, including trouble sleeping, sleep disorder, and insufficient (<6 h/day) or excessive (>9 h/day) sleep, were examined. Weighted multiple linear/logistic regression models were constructed to estimate the linear associations of VOCs exposure with sleep health. As for survival analysis of different VOCs related with individual survival outcomes, we selected the weighted multivariate COX regression model to assess. To explore the non-linear association between variables, restricted cubic spline (RCS) regression analysis was carried out. Moreover, three adjustment regression modeling strategies were utilized to evaluate the VOCs effect on sleep health.
Results
According to the adjusted linear RCS regression model, it is reported that the U-shaped nonlinear relationship between blood VOCs (LBXVBM: P-overall = 0.0284; LBXVCM: P-overall = 0.0321) and sleep duration. The fully adjusted logistic RCS model revealed that LBXVBM, LBXVCM and LBXVCF all displayed statistically significant U-shaped curves in trouble sleep and insufficient sleep groups (P-overall < 0.05). The adjusted COX-RCS analysis results revealed that the LBXVME (p-overall = 0.0359) was risk factor for all-cause mortality.
Conclusions
There was a negative non-linear association between specific blood VOCs and sleep health among U.S. adults, and this adverse effect was mainly manifested in trouble sleeping and prolonged sleep duration. Moreover, the results of survival analysis showed that environmental VOCs exposure could induce adverse survival outcomes. Future prospective longitudinal studies should be conducted to further investigate and determine the degree of the association between VOCs and sleep health.
A study on the impact of life meaning on occupational identity of nursing students in China:A cross-sectional study
Authors: Qiu Lingjing , Miao Qunfang , Zhao YueYan , Zhu Li , Wang Yanling
Published: October 2023

Background
With the alterations of the Chinese epidemic prevention policy, China experienced a nationwide wave of the Coronavirus disease 2019 (COVID-19) pandemic from December 2022 to January 2023.The impact of the COVID-19 pandemic extends beyond individual perceptions of the meaning of life and attitudes toward life and death; it also affects the professional identity of nursing students.This study explored nursing students' professional identity and life meaning of affected by the pandemic.
Methods
An online survey was conducted using the Chinese version of the Nursing Student Career Identity Scale and the Chinese version of the Meaning in Life Questionnaire to assess the current status of nursing students' professional identity and sense of life meaning. Data were also collected on participant gender, education, clinical practice experience, and COVID-19 infection.
Results
The scores for nursing students' sense of professional identity (61.58±16.16) and sense of life meaning (45.29±12.65) were both at an intermediate level. Compared to the scores before the COVID-19 pandemic, Chinese nursing students' professional identity scores increased, while their sense of life meaning scores decreased. Moreover, we found a positive correlation between professional identity and sense of life meaning (p<0.001); nursing students exhibited a stronger professional identity when they had a relatively higher sense of life meaning than those with a relatively lower sense of life meaning.
Conclusion
Enhancing nursing students' sense of meaning in life is crucial for maintaining their professional identity. Attention should be given to life education for nursing students and the development of relevant educational curricula.
Development of Communication Systems for a Photovoltaic Plant with Battery Energy Storage System and All-Sky Camera
Authors: Haas Lucas , Gehrke Camila Seibel , de Lucena Micael Praxedes , Santos Julia Alves , Cavalcante Sidnéia Lira , Hartmann Lucas Vinícius , Gomes Flávio da Silva Vitorino , Silva Italo Roger Ferreira Moreno Pinheiro da
Published: October 2023

The efficient operation, monitoring, and maintenance of a photovoltaic (PV) plant are intrinsically linked to data accessibility and reliability, which, in turn, rely on the robustness of the communication system. As new technologies arise and newer equipment is integrated into the PV plants, such as the battery energy storage system (BESS) that transform the PV plant into a dispatchable plant and the all-sky camera (ASC) that enables the prediction of shading events, the communication system faces new challenges, that are described in this work. In this paper, two communication systems were developed using only open-source software, in which the first was designed for seamless communication between the PV and BESS equipment, while the second was tailored to collect photos from ASC devices. The requirements of the communication systems were defined based on the applications that control the PV plant, and on the industry-standard IEC-61724-1 norm for PV data. After being developed, the communication systems were installed in a PV plant, and the interaction between the data obtained from these two systems is discussed and presented.
Identification of a pyroptosis-immune-related lncRNA signature for prognostic and immune landscape prediction in bladder cancer patients
Authors: Zhao Fuguang , Jia Zhibo , Xie Hui
Published: October 2023

Purpose
Individualized medicine has become increasingly important in bladder cancer treatment, whereas useful biomarkers for prognostic prediction are still lacking. The current study, therefore, constructed a novel risk model based on pyroptosis- and immune-related long noncoding RNAs (Pyro-Imm lncRNAs) to evaluate the potential prognosis of bladder cancer.
Methods
Corresponding data of bladder cancer patients were downloaded from the Cancer Genome Atlas (TCGA) database. The univariate Cox regression analysis, least absolute shrinkage and selection operator (LASSO) regression analysis, and multivariate Cox regression analysis were employed to establish a predictive signature, which was evaluated by receiver operator characteristic (ROC) analysis and Kaplan–Meier analysis. Furthermore, the immune infiltration, immune checkpoints, and responses to chemotherapeutic drugs were analyzed with this model.
Results
Three Pyro-Imm lncRNAs (MAFG-DT, AC024060.1, AC116914.2) were finally identified. Patients in the low-risk group demonstrated a significant survival advantage. The area under the ROC curve (AUC) at 1, 3, and 5 years was 0.694, 0.709, and 0.736 respectively in the entire cohort. KEGG and GO analyses showed that the Wnt pathway plays a crucial role in the high-risk group. The risk score was significantly related to the degree of infiltration of different immune cells, the expression of multiple immune checkpoint genes, and the sensitivity of various chemotherapeutic drugs.
Conclusion
This novel signature provides a theoretical basis for cancer immunology and chemotherapy, which might help develop individualized therapy.
Impact of ablation heat sink effect on the lesion range after liver MWA at different times and powers
Authors: He Ping , Tang Xiao-Qing , Yang You , Luo Xia , Yang Xin , Li Yang , Yu Jin-Hong
Published: October 2023

Background During microwave ablation (MWA) treatment, the heat sink effect can cause incomplete ablation. We discussed the influence of the effect by comparing the lesion ranges of in vitro and vivo.Method Twenty rabbits were divided into four groups with MWA power and time set at 20W-1min, 20W-3min, 30W-1min, and 30W-3min. Compare the range of ablation lesions in vivo and in vitro under a microscope.Results The 20W-1min group and the 30W-3min group: there were not differences in the sizes of each diameter line of the lesion in vivo and in vitro (P > 0.05). The 30W-1min group: there were statistically significant differences in the major and minor axis and the area of lesion including edema zone (P < 0.05), while other diameter lines were not statistically significant differences (P > 0.05); The 20W-3min group: showed statistically significant differences in the major and minor axis and the area of coagulation necrosis lesion and lesion including edema zone (P < 0.05), while the size of circular oedema zone was not differences (P > 0.05).Conclusion The heat sink effect becomes more pronounced as the heat increases. However, after exceeding the thermal damage threshold that the tissue can withstand, the influence of the heat sink effect on MWA lesions will gradually weaken.
A linear optimization approach to decision support with CANS data
Authors: Offner David , Warner Dan , Yi Cara , Zhang Wani
Published: October 2023

This research is meant to make a contribution to the field of Communimetric (CMX) algorithms (Lyons, 2004, 2009). This is the science underscoring the behavioral health and child welfare assessment tools such as the Child and Adolescent Needs and Strengths (CANS) assessment, the Adult Needs and Strengths Assessment (ANSA), and the Family Advocacy and Support Tool (FAST), amongst others (https://praedfoundation.org/). These tools are used throughout the US child serving and adult mental health system not only to assure a standardized assessment process, but also to provide real-time Decision Support (DS) to assessors about client treatment planning and disposition. We propose a novel method for developing CMX DS utilizing linear optimization (commonly known also as linear programming (LP) or (mixed) integer linear programming (ILP or MILP)). Using administrative data from Pennsylvania’s Child Medicaid Behavioral Health system, a MILP model is developed to differentiate profiles that benefit from one of two programs in the Pennsylvania child system of care. The programs picked are both for children at risk of out of home placement, but their clinical models are different. There is often debate in the field as to which program is most appropriate for a given family, and thus the MILP model is meant to help facilitate this decision making. We propose both a method for developing a MILP model in Medicaid behavioral health, as well as a method for testing and validating the model. We then propose a method for identifying the impact on care that utilization of the model could have in facilitating improved care matching in a system. We close with a discussion of how this information could also be used for system planning, and program development issues.
CCCP ameliorated TGF-β1 mediated epithelial-mesenchymal transition in pulmonary fibrosis through the upregulation of PINK1/Parkin-mediated mitophagy
Authors: Ding Gaofeng , Duan Qingke , Wang Wen , Yuan Junhui , Xu Wencai , Lu Yufei
Published: October 2023

In recent years, mitophagy has been proved to be involved in the development of IPF. Nevertheless, the role of PINK1/Parkin-dependent mitophagy in airway epithelial cell injury during the development of IPF has not been sufficiently clarified. Herein, we investigated the effects of PINK1/Parkin-dependent mitophagy and mitochondrial function-regulated epithelial-mesenchymal transaction (EMT) in airway epithelial A549 cells exposed to transforming growth factor-β1 (TGF-β1). TGF-β1 seriously decreased PINK1, Parkin and Beclin‐1protein expression levels and increased the P62 protein expression level, and thus suppressing mitophagy in A549 cells. Meanwhile, the expression levels of E-cadherin and CK19 significantly increased and the N-cadherin and vimentin expression levels decreased with increasing TGF-β1 doses, which further aggravated the EMT phenomenon. Furthermore, transmission electron microscope showed that the numbers of autophagosomes and autophagolysosomes were markedly decreased. Meanwhile, Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the agonist of PINK1, enhanced the PINK1, Parkin and Beclin‐1protein expression and decreased the P62 protein expression. Confocal microscopy analysis also revealed that CCCP notably enhanced the LC3 protein expression and decreased the FN1 protein expression. CCCP also markedly decrease the ROS level. The expression levels of E-cadherin and CK19 increased and the expression levels of N-cadherin and vimentin decreased after treatment with CCCP. In conclusion, our study confirmed that the PINK1/Parkin-dependent mitophagy deficiency promotes alveolar epithelial cell EMT induced by TGF-β1 and may be involved in the pathogenesis of IPF. CCCP attenuated on TGF-β-induced cell mitophagy and EMT. These findings provide a new experimental basis and target for the treatment of IPF.
Efficient schemes of classifiers for Remote Sensing Satellite Imageries of land use pattern classifications
Authors: Udupi Sachidananda , HC Hemamalini , Kumar Vineet , Chittora Vaibhav , D.K.Prabhuraj  , Patil S.S.
Published: October 2023

Land use pattern classification of remote sensing imagery data is imperative to research that is used in remote sensing applications. Remote sensing (RS) technologies were exploited to mine some of the significant spatially variable factors, such as land cover and land use (LCLU), from satellite images of remote arid areas in Karnataka, India. Four diverse classification techniques, unsupervised and supervised (Maximum likelihood, Mahalanobis Distance, and Minimum Distance) are applied in Bellary district in Karnataka, India for the classification of raw satellite images. The developed maps are then visually compared with each other and accuracy evaluations made using ground-truths are carried out. It was found that the Maximum likelihood technique gave the finest results and both Minimum distance and Mahalanobis distance methods overvalued agricultural land areas. In spite of missing a few insignificant features due to the low resolution of the satellite images, a high-quality accord between parameters extracted automatically from the developed maps and field observations was found.
Assessing the Need for Values Counselling Among Higher Education Students in India: a Survey Study
Authors: SWARGIARY KHRITISH
Published: October 2023

This survey study aims to assess the need for values counselling among higher education students in India. The study aims to examine the impact of counselling interventions on students' values, attitudes, and behaviors. By understanding the potential benefits of values counselling, educators and policymakers can design effective intervention programs to promote holistic development among Indian higher education students.
The new mineral cuprozheshengite, Pb4CuZn2(AsO4)2(PO4)2(OH)2, from Yunnan, China, with site-selective As-P substitution
Authors: Sun Ningyue , Grey Ian E. , Li Guowu , Rewitzer Christian , Xue Yuan , Mumme William G. , Shen Hongtao , Hao Jinhua , MacRae Colin M. , Riboldi-Tunnicliffe Alan , Boer Stephanie , Williams Tim , Kampf Anthony R.
Published: October 2023

Cuprozheshengite, Pb4CuZn2(AsO4)2(PO4)2(OH)2, is a new mineral species from Yunnan, China. It occurs as sub-millimeter greenish-blue hemispherical aggregates of microscopic blade-like crystals on hemimorphite and is closely associated with veszelyite and galena. Cuprozheshengite is brittle with irregular fracture and has a Mohs hardness of 2½–3 and perfect cleavages on {011}. The calculated density is 5.91 g/cm3. The empirical chemical formula of the holotype is (Pb3.97Na0.04Ca0.01)Σ4.02Cu1.06 Zn2.09(AsO4)2[(P0.84As0.12Si0.01)Σ0.97O4]2(OH)2 based on 18 O atoms per formula unit. Cuprozheshengite is triclinic, space group P1, with unit-cell parameters a = 4.7977(8), b = 8.5789(8), c = 10.3855(9) Å, α = 97.270(8)°, β = 101.902(12)°, γ = 91.495(11)°, V = 414.30(9) Å3, and Z = 1. Cuprozheshengite is a member of dongchuanite group, whose general formula is A4VIBIVB2(X1O4)2(X2O4)2(OH)2, where A is an interlayer cation with Pb being dominant; B are transition metals with two crystallographic positions, IVB has tetrahedral coordination and is fully occupied by Zn, while VIB has octahedral coordination and is dominated by Zn or Cu; X1 and X2 are cations with tetrahedral coordination, occupied by As and P. Like other dongchuanite group minerals, the structural framework of cuprozheshengite is composed of two heteropolyhedral columns along [100]. Type 1 columns comprise corner-linked [IVBO4] and [X2O4] tetrahedra. Each tetrahedron is connected with three other tetrahedra in the columns. Type 2 columns have alternating [VIBO4(OH)2] octahedra with pairs of corner-connected [X1O4] tetrahedra. These two columns are connected by corner-sharing between [IVBO4] and [X1O4] tetrahedra to form layers parallel to (011). Pb atoms occupy two independent sites between the layers. Cuprozheshengite is named as the copper analog of zheshengite. Single-crystal X-ray diffraction reveals that As and P order over the X1 and X2 sites, with As tending to occupy X1. Density functional theory (DFT) calculations confirm the occupancy propensity of As benefiting structural stability. The structural and stability studies of cuprozheshengite may have implications for local environmental governance. As a stable mineral in the water and elemental cycles after weathering, cuprozheshengite still has the potential to continually crystallize, fixing As into a stable crystalline waste form.
Sandıklı Müfrezesi ve Faaliyetleri Sandıklı
Authors: ALTINTAŞ Ahmet
Published: October 2023

İzmir’in işgali sonrasında Anadolu’nun her yerinde işgallere karşı bir farkındalık oluşmuş çareler aranmaya başlanmıştı. Afyonkarahisar’a bağlı Sandıklı ilçesi de Yunan işgaline karşı en çok tepki gösterilen yerleşim merkezlerinden birisiydi. İşgalin ardından Sandıklı Kuva-yı Milliye Teşkilatı oluşturulmuş Sandıklılılar ellerinden gelen her türlü yardımı yapmışlardı. Sandıklı Kuva-yı Milliye Teşkilatı’nın Yunan işgali karşısındaki faaliyetlerinden birisi de bir piyade ve süvari müfrezesi teşkil etmek olmuştu. İlerleyen dönemde bu teşkilatlar daha sonra Sandıklı Müfrezesi’ne dönüştürülmüştü. Sandıklı Müfrezesi, İkinci İnönü Savaşı’ndan önce bölge askerlik şubeleri tarafından kurgulaması yapılmış daha sonra Afyonkarahisar Askerlik Şubesi’nce 20 Mayıs 1921 tarihinde yeniden teşkilatlandırılmıştı. Sandıklı Müfrezesi, üç taburlu bir piyade alayı, üç bölüklü bir süvari alayı, iki toplu bir dağ bataryasından meydana gelmişti. Sandıklı Müfrezesi daha sonra düzenli orduya katılarak Milli Mücadele’deki faaliyetlerini İzmir’in alınışına kadar devam ettirmiştir.
Bitle Yayılan Felaket: Tifüs
Authors: KARCI Erol
Published: October 2023

Tifüs, insanlık tarihinin en eski hastalıklarındandır. Tarihi ve coğrafi kökenleri tartışmalı olmakla birlikte bazı tıp tarihçileri tifüsün Thucydides tarafından tanımlanan eski bir Avrupa hastalığı olduğunu ileri sürmektedirler. Vücut biti “Pediculus humanus” ile bulaşan tifüsün belirtileri arasında yüksek ateş, halsizlik, zihinsel karışıklık ve karakteristik bir döküntü bulunmaktadır. Hastalık, vücut bitinin çoğalmasına ve geçişine elverişli koşullarda özellikle de soğuk, aşırı kalabalık ve sağlıksız yaşam koşullarında gelişir. Bu çalışma Avrupa tarihine yön veren bazı savaşlarda kendisini gösteren ve savaşın sonucuna etki eden tifüs salgınları hakkında bilgi vermeyi amaçlamaktadır. Bu sayede bir hastalığın siyasi, askeri ve sosyal etkileri ortaya konulmaya çalışılacaktır. Tifüs salgınlarının birçok savaşta etkili olduğu bilinmektedir. Çalışmamızda bunlardan Granada Savaşları, Habsburg-Valois mücadelesi, Osmanlı-Macar savaşları, Otuz Yıl Savaşları, Napolyon Savaşları, Kırım Savaşı, 1877-1878 Osmanlı-Rus Savaşı, I. Dünya Savaşı yıllarında Sırbistan’daki tifüs salgını ve Rusya İç Savaşı (1918-1922) sırasındaki tifüs salgını ele alınmaya çalışılmıştır.
Modeling of water table profile variations owing to stream–aquifer interaction
Authors: Upadhyaya Ashutosh , Kankarej Manisha M. , Jeet Pawan
Published: October 2023

Spatial and temporal variations of the water table could be explained by the one-dimensional Boussinesq equation by incorporating the variables of evapotranspiration and groundwater recharge with appropriate initial and boundary conditions. In this study, the stream–aquifer interaction has been investigated through a numerical example model with the implementations of Galerkin's method-based Finite Element Solution (FES), Hybrid Finite Analytic Solution (HFAS), Fully Implicit Finite Difference Solution (FIFDS) of one-dimensional nonlinear Boussinesq equation, and analytical solutions of the Boussinesq equation linearized by Baumann's transformation (AS I) as well as linearized by Werner's transformation (AS II). Considering HFAS as the benchmark solution, it was observed that in both recharging and discharging aquifers, water table profiles at 1 day and 5 days as obtained from FES followed by FIFDS were observed quite close to HFAS. Based on L2 and Tchebycheff norms, FES and FIFDS were ranked in first and second place, respectively. L2 and Tchebycheff norms could not consistently establish the performance ranking of analytical solutions but their performance ranking was certainly below the numerical solutions. The performance ranking of analytical solutions could not consistently be established using the L2 and Tchebycheff norms, but it was certainly below the numerical solutions.
The Effects of Statin Therapy on Circulating Levels of
Trimethylamine N-oxide: A Systematic Review and Meta-analysis
Authors: Jamialahmadi Tannaz , Reiner Željko , Matbou Riahi Maryam , Kesharwani Prashant , Eid Ali H. , Tayarani-Najaran Zahra , Sahebkar Amirhossein
Published: October 2023

Background:

Gut microbiota is associated with an increased risk of atherosclerotic
cardiovascular disease (ASCVD) through the metabolites, which can induce
atherogenesis. One of these metabolites is trimethylamine N-oxide (TMAO). Some
studies indicate that statins do not only decrease LDL-cholesterol and thus ASCVD risk,
but they also affect gut microbiota. There are only a few studies on humans suggesting
that statins might also decrease TMAO, but their results are not unanimous. This
meta-analysis aimed to provide an answer as to whether statins do affect decreasing the
plasma levels of atherogenic TMAO.

Methods:

A systematic literature search in PubMed, Scopus, Embase, and Web of Science
was performed from inception to January 1st, 2023. To assess the quality of each
study included in the meta-analysis, the Cochrane Quality Assessment tool 1 (ROB 1)
was used. Comprehensive Meta-Analysis V3 software was used to perform the metaanalysis.
The weighted mean difference was also used. A random effects meta-analysis
was used to calculate the overall estimate of effect size. In the leave-one-out approach,
one study was excluded from each analysis to evaluate the effect of each study on the
overall effect size.

Results:

Random-effects meta-analysis of 3 studies including 244 patients demonstrated
a significant decrease in plasma TMAO levels after statin treatment (WMD: -1.839,
95% CI: -2.391, -1.287, p<0.001; I2:0). The reduction in TMAO was robust in the leaveone-
out sensitivity analysis.

Conclusion:

Statins might reduce TMAO levels, but there is a need for further evidence
from long-term studies taking into account different types and doses of statins.
Effects of Consuming Repeatedly Heated Edible Oils on Cardiovascular Diseases: A Narrative Review
Authors: Soundararajan Prathyusha , Parthasarathy Srividya , Sakthivelu Meenakumari , Karuppiah Kanchana Mala , Velusamy Palaniyandi , Gopinath Subash Chandra Bose , Raman Pachaiappan
Published: October 2023

:

Edible oils are inevitable requisites in the human diet as they are enriched with
essential fatty acids, vitamins, carotenoids, sterols, and other antioxidants. Due to their nutritive
value and commercial significance, edible oils have been used for food preparation for
many centuries. The use of global consumption of edible oils has dramatically increased
throughout the world in the 21st century owing to their incredible application in all kinds of
food preparation. However, a variety of pollutants, such as pesticides, toxic chemicals,
heavy metals, and environmental pollution, have contributed to the contamination of edible
oils. Furthermore, the benzophenanthridine alkaloids, sanguinarine, dihydrosanguinarine,
butter yellow, and other several agents are added intentionally, which are known to cause a
number of human diseases. Apart from this, repeated heating and reusing of oils results in
trans fats, and lipid peroxidation alters the fatty acid composition, which adversely affects
the health of consumers and increases the risk of cardiovascular diseases. Moreover, the
prevention of edible oil contamination in human health at various levels is inevitable to ensure
consumer safety. Hence, the present review provides an overview of vegetable cooking
oils and the health ailments that detection techniques are focused on.
SGLT-2 Inhibitors: The Next-generation Treatment for Type 2
Diabetes Mellitus
Authors: Lukic Nikola , Macvanin Mirjana T. , Gluvic Zoran , Rizzo Manfredi , Radak Djordje , Suri Jasjit S. , Isenovic Esma R.
Published: October 2023

:

Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent
years, primarily in highly developed Western societies. T2DM causes systemic complications,
such as atherosclerotic heart disease, ischemic stroke, peripheral artery disease,
kidney failure, and diabetes-related maculopathy and retinopathy. The growing number
of T2DM patients and the treatment of long-term T2DM-related complications pressurize
and exhaust public healthcare systems. As a result, strategies for combating T2DM
and developing novel drugs are critical global public health requirements. Aside from
preventive measures, which are still the most effective way to prevent T2DM, novel and
highly effective therapies are emerging. In the spotlight of next-generation T2DM treatment,
sodium-glucose co-transporter 2 (SGLT-2) inhibitors are promoted as the most efficient
perspective therapy. SGLT-2 inhibitors (SGLT2i) include phlorizin derivatives,
such as canagliflozin, dapagliflozin, empagliflozin, and ertugliflozin. SGLT-2, along
with SGLT-1, is a member of the SGLT family of proteins that play a role in glucose absorption
via active transport mediated by Na+/K+ ATPase. SGLT-2 is only found in the
kidney, specifically the proximal tubule, and is responsible for more than 90% glucose
absorption. Inhibition of SGLT-2 reduces glucose absorption, and consequently increases
urinary glucose excretion, decreasing blood glucose levels. Thus, the inhibition of
SGLT-2 activity ultimately alleviates T2DM-related symptoms and prevents or delays
systemic T2DM-associated chronic complications. This review aimed to provide a more
detailed understanding of the effects of SGLT2i responsible for the acute improvement
in blood glucose regulation, a prerequisite for T2DM-associated cardiovascular complications
control.
Keywords: Type 2 diabetes mellitus, T2DM,
In Silico Screening and Molecular Dynamics Simulations against Tyrosine-protein Kinase Fyn Reveal Potential Novel Therapeutic Candidates for Bovine Papillomatosis
Authors: Barros Gerlane Salgueiro , Barreto Débora Machado , Cavalcanti Sandy Gabrielly Souza , Oliveira Tiago Branquinho , Rodrigues Ricardo Pereira , de Aragão Batista Marcus Vinicius
Published: October 2023

Background:

Decreased beef productivity due to papillomatosis has led to
the development and identification of novel targets and molecules to treat the disease.
Protein kinases are promising targets for the design of numerous chemotherapy drugs.

Objective:

This study aimed to screen and design new inhibitors of bovine Fyn, a protein
kinase, using structure-based computational methods, such as molecular docking
and molecular dynamics simulation (MDS).

Methods:

To carry out the molecular docking analysis, five ligands obtained through
structural similarity between active compounds along with the cross-inhibition function
between the ChEMBL and Drugbank databases were used. Molecular modeling was performed,
and the generated models were validated using PROCHECK and Verify 3D.
Molecular docking was performed using Autodock Vina. The complexes formed between
Fyn and the three best ligands had their stability assessed by MDS. In these simulations,
the complexes were stabilized for 100 ns in relation to a pressure of 1 atm, with
an average temperature of 300 k and a potential energy of 1,145,336 kJ/m converged in
997 steps.

Results:

Docking analyses showed that all selected ligands had a high binding affinity
with Fyn and presented hydrogen bonds at important active sites. MDS results support
the docking results, as the ligand showed similar and stable interactions with amino
acids present at the binding site of the protein. In all simulations, sorafenib obtained the
best results of interaction with the bovine Fyn.

Conclusion:

The results highlight the identification of possible bovine Fyn inhibitors;
however, further studies are important to confirm these results experimentally.
LncRNA MEG3: Targeting the Molecular Mechanisms and Pathogenic causes of Metabolic Diseases
Authors: Luo Yiyang , Wang Hailin , Wang Lijun , Wu Wei , Zhao Jiale , Li Xueqing , Xiong Ruisi , Ding Xueliang , Yuan Ding , Yuan Chengfu
Published: October 2023

Background:

Non-coding RNA is a type of RNA that does not encode proteins,
distributed among rRNA, tRNA, snRNA, snoRNA, microRNA and other RNAs
with identified functions, where the Long non-coding RNA (lncRNA) displays a nucleotide
length over 200. LncRNAs enable multiple biological processes in the human
body, including cancer cell invasion and metastasis, apoptosis, cell autophagy, inflammation,
etc. Recently, a growing body of studies has demonstrated the association of lncRNAs
with obesity and obesity-induced insulin resistance and NAFLD, where MEG3 is
related to glucose metabolism, such as insulin resistance. In addition, MEG3 has been
demonstrated in the pathological processes of various cancers, such as mediating inflammation,
cardiovascular disease, liver disease and other metabolic diseases.

Objective:

To explore the regulatory role of lncRNA MEG3 in metabolic diseases. It provides
new ideas for clinical treatment or experimental research.

Methods:

In this paper, in order to obtain enough data, we integrate and analyze the data
in the PubMed database.

Results:

LncRNA MEG3 can regulate many metabolic diseases, such as insulin resistance,
NAFLD, inflammation and so on.

Conclusion:

LncRNA MEG3 has a regulatory role in a variety of metabolic diseases,
which are currently difficult to be completely cured, and MEG3 is a potential target for
the treatment of these diseases. Here, we review the role of lncRNA MEG3 in mechanisms
of action and biological functions in human metabolic diseases.
Cathepsin D Attenuates the Proliferation of Vascular Smooth Muscle Cells
Induced by the AGE/RAGE Pathway by Suppressing the ERK Signal
Authors: Ye Ning , Miao Linlin , Wang Fengzhi , Wu Shaojun , Wu Boquan , Zhou Ying , Wang Chang , Sun Guozhe
Published: October 2023

Background:

In this study, we aimed to clarify the role and mechanism by which Cathepsin D
(CTSD) mediates the advanced glycation end products (AGEs)-induced proliferation of vascular smooth muscle
cells (VSMCs).

Methods:

We conducted a Western blotting assay and co-immunoprecipitation assay to detect the expression
of target proteins and the interaction between different proteins. Cell Counting Kit-8 (CCK-8) assay and 5-
ethynyl-2’-deoxyuridine (EdU) were used to evaluate the proliferation.

Results:

AGEs significantly promoted phenotypic switching and proliferation of VSMCs in a concentration-dependent
manner. This effect of AGEs was accompanied by inhibition of CTSD. Both the proliferation of
VSMCs and inhibition of CTSD induced by AGEs could be attenuated by the specific inhibitor of the receptor
for advanced glycation end products (RAGE), FPS-ZM1. Overexpression of CTSD significantly alleviated these
effects of AGEs on VSMCs. The mechanism of CTSD action in VSMCs was also explored. Overexpression
of CTSD reduced the activation of p-ERK caused by AGEs. By contrast, the knockdown of CTSD, elicited
using a plasmid containing short hairpin RNA (shRNA) against CTSD, further increased the activation of
p-ERK compared to AGEs alone. Additionally, co-immunoprecipitation studies revealed an endogenous interaction
between CTSD, a protease, and p-ERK, its potential substrate.

Conclusion:

It has been demonstrated that CTSD downregulates the level of phosphorylated ERK by degrading
its target, and this interaction plays a critical role in the proliferation of VSMCs induced by the
AGE/RAGE axis. These results provide a novel insight into the prevention and treatment of vascular complications
in diabetes.
Raddeanin A Improves the Therapeutic Effect of Osimertinib in NSCLC by
Accelerating ROS/NLRP3-mediated Pyroptosis
Authors: Lin Liping , Wu Xuan , Jiang Yuanxue , Luo Xi , Cao Xiaolong
Published: October 2023

Background:

Osimertinib (Osm) is the preferred treatment for non-small cell lung cancer (NSCLC)
patients with the epidermal growth factor receptor (EGFR) T790M mutation. Nevertheless, the resistance of
NSCLC cells to Osm will eventually develop, which remains the biggest obstacle to treating such diseases.
Raddeanin A (RA) exhibits a potent anti-tumor effect on various types of cancer cells. In this study, we aimed
to investigate whether RA suppresses NSCLC growth and increases the therapeutic effect of Osm.

Methods:

The effects of RA on inhibiting NSCLC cell viability and proliferation were tested using cell counting
kit 8 (CCK-8) and EdU assay. The roles of RA in improving the anti-tumor effect of Osm were tested with
CCK-8 and colony formation assays. The roles of RA in regulating reactive oxygen species (ROS)/NOD-, LRR-,
and pyrin domain-containing protein 3 (NLRP3)-mediated pyroptosis were assessed using quantitative real-
time PCR (qRT-PCR) and western blotting analysis.

Results:

RA treatment decreased A549 and H1975 cell viability in a dose- and time-dependent way. RA inhibited
NSCLC cell proliferation and tumor growth in vivo. Mechanistically, RA induced ROS overgeneration
and resulted in subsequent NLRP3-mediated pyroptosis. In particular, combination treatment with Osm and
RA reduced cell viability and clonogenic growth capacity more efficiently than Osm mono treatment in A549
and H1975 cells. Combination treatment also promoted NLRP3-mediated pyroptosis more efficiently than
Osm mono treatment.

Conclusion:

RA inhibited the NSCLC growth and increased the anti-tumor role of Osm in NSCLC by facilitating
ROS/NLRP3-mediated pyroptosis. These results suggested that combination therapy with RA and Osm
might be an effective strategy to treat Osm-resistant NSCLC.
Review of Analytical Methods for Evaluating Azithromycin in the Context of
Green Analytical Chemistry
Authors: Sinzervinch Aline , Lustosa Isadora Alves , Kogawa Ana Carolina
Published: October 2023

Background:

Azithromycin (AZT) is an antimicrobial available in different pharmaceutical forms
and many people can have access to this medicine. Therefore, the existence of adequate and reliable analytical
methods for evaluating the quality of AZT and AZT-based products is essential.

Objective/Methods:

The purpose of this review is to discuss the analytical methods for evaluating AZT present
in the literature and official compendia in the context of Green Analytical Chemistry (GAC).

Results:

Among the methods found in the literature for evaluating AZT, the most used method is HPLC (62%)
followed by TLC (14%) and the microbiological method by agar diffusion (14%). Even pharmacopoeias recommend
the analysis of AZT by HPLC or agar diffusion. Acetonitrile and methanol account for 35% of the
most used solvents in the analyses, followed by buffer.

Conclusion:

AZT lacks analytical methods in the context of GAC. Both physical-chemical and microbiological
methods can contemplate the environmentally friendly way to analyze AZT and AZT-based products, depending
only on the chosen conditions. Ethanol, purified water, acetic acid instead of methanol, acetonitrile,
buffer, formic acid in the physical-chemical methods are excellent alternatives. However, in the microbiological
method, turbidimetry is a great option instead of agar diffusion.
Transcriptomic Analysis of THP-1 Cells Exposed by Monosodium Urate Reveals Key Genes Involved in Gout
Authors: Wang Guozhen , Liu Zijia , Zheng Yuchen , Sheng Chao , Hou Xiaonan , Yao Mengfei , Zong Qi , Tang Duo , Zhou Zhixiang , Zhang Tie , Yang Yishu
Published: October 2023

Background:

Gout is a common inflammatory arthritis, which is mainly caused by
the deposition of monosodium urate (MSU) in tissues. Transcriptomics was used to explore the
pathogenesis and treatment of gout in our work.

Objective:

The objective of the study was to analyze and validate potential therapeutic targets
and biomarkers in THP-1 cells that were exposed to MSU.

Methods:

THP-1 cells were exposed to MSU. The inflammatory effect was characterized, and
RNA-Seq analysis was then carried out. The differential genes obtained by RNA-Seq were analyzed with gene expression omnibus (GEO) series 160170 (GSE160170) gout-related clinical
samples in the GEO database and gout-related genes in the GeneCards database. From the three
analysis approaches, the genes with significant differences were verified by the differential
genes’ transcription levels. The interaction relationship of long non-coding RNA (lncRNA) was
proposed by ceRNA network analysis.

Results:

MSU significantly promoted the release of IL-1β and IL-18 in THP-1 cells, which aggravated their inflammatory effect. Through RNA-Seq, 698 differential genes were obtained, including 606 differential mRNA and 92 differential `LncRNA. Cross-analysis of the RNA-Seq
differential genes, the GSE160170 differential genes, and the gout-related genes in GeneCards
revealed a total of 17 genes coexisting in the tripartite data. Furthermore, seven differential
genes—C-X-C motif chemokine ligand 8 (CXCL8), C-X-C motif chemokine ligand 2 (CXCL2),
tumor necrosis factor (TNF), C-C motif chemokine ligand 3 (CCL3), suppressor of cytokine
signaling 3 (SOCS3), oncostatin M (OSM), and MIR22 host gene (MIR22HG)—were verified
as key genes that analyzed the weight of genes in pathways, the enrichment of inflammationrelated pathways, and protein-protein interaction (PPI)nodes combined with the expression of
genes in RNA-Seq and GSE160170. It is suggested that MIR22HG may regulate OSM and
SOCS3 through microRNA 4271 (miR-4271), OSM, and SOCS3m; CCL3 through microRNA
149-3p (miR-149-3p); and CXCL2 through microRNA 4652-3p (miR-4652-3p).

Conclusion:

The potential of CXCL8, CXCL2, TNF, CCL3, SOCS3, and OSM as gout biomarkers and MIR22HG as a therapeutic target for gout are proposed, which provide new insights into the mechanisms of gout biomarkers and therapeutic methods.
Qingrexiaoji Recipe Regulates the Differentiation of M2 TAM via miR-29 in GC
Authors: Zhang Yiqiong , Chen Luting , Fei Yuchang , Chen Peifeng , Pan Lei
Published: October 2023

Background:

Gastric cancer, one of the most familiar adenocarcinomas of the gastrointestinal
tract, ranks third in the world in cancer-related deaths. Traditional Chinese medicine
can suppress the growth of tumors, and the underlying mechanism may be associated with
the tumor microenvironment. Here, we investigated the anti-cancer effects of the Qingrexiaoji
recipe on gastric cancer and the underlying molecular mechanism.

Methods:

An in vivo nude mouse model was established, and the expression of CD206, CD80,
and M2 phenotype-related proteins (Arg-1, Fizz1) was obtained by flow cytometry and western
blotting. The expressions of the M2 phenotype-related cytokines were examined by ELISA.

Results:

Qingrexiaoji recipe inhibited gastric tumor growth and downregulated the expression
of CD206, IFN-γ, IL-13, IL-4, and TNF-α in vivo. Qingrexiaoji recipe deceased M2 phenotypic
polarization by upregulating microRNA (miR)-29a-3p level. Luciferase activity assays showed
that HDAC4 is a potential target of miR-29a-3p. In cells co-transfected with HDAC4 siRNA and
miR-29a-3p inhibitor and treated with IL-4 and Qingrexiaoji recipe, the miR-29a-3p inhibitorinduced
increase of M2 phenotypic polarization was reversed.

Conclusion:

In summary, these results suggested that the Qingrexiaoji recipe regulated M2
macrophage polarization by regulating miR-29a-3p/HDAC4, providing a different and innovative
treatment for gastric cancer.
The Treatment of Tubal Inflammatory Infertility using Yinjia Tablets
through EGFR/MEK/ERK Signaling Pathway based on Network
Pharmacology
Authors: Huang Yefang , He Zhelin , Zhou Hang , Wen Yi , Ji Xiaoli , Ding Weijun , Zhu Boyu , Zhang Yongqing , Tan Ying , Yang Kun , Wang Yan
Published: October 2023

Background:

Salpingitis obstructive infertility (SOI) refers to infertility caused by abnormal
conditions such as tubal adhesion and blockage caused by acute and chronic salpingitis.
SOI has a serious impact on women's physical and mental health and family harmony, and it is a
clinical problem that needs to be solved urgently.

Objective:

The purpose of the present study was to explore the potential pharmacological mechanisms
of the Yinjia tablets (Yin Jia Pian, YJP) on tubal inflammation.

Methods:

Networks of YJP-associated targets and tubal inflammation-related genes were constructed
through the STRING database. Potential targets and pathway enrichment analysis related
to the therapeutic efficacy of YJP were identified using Cytoscape and Database for Annotation,
Visualization, and Integrated Discovery (metascape). E. coli was used to establish a rat model of
tubal inflammation and to validate the predictions of network pharmacology and the therapeutic
efficacy of YJP. H&E staining was used to observe the pathological changes in fallopian tubes.
TEM observation of the ultrastructure of the fallopian tubes. ELISA was used to detect the changes
of IL-6 and TNF-α in fallopian tubes. Immunohistochemistry was used to detect the expression of
ESR1. The changes of Bcl-2, ERK1/2, p-ERK1/2, MEK, p-MEK, EGFR, and p-EGFR were detected
by western blot.

Results:

Through database analysis, it was found that YJP shared 105 identical targets with the
disease. Network pharmacology analysis showed that IL-6, TNF, and EGFR belong to the top 5
core proteins associated with salpingitis, and EGFR/MEK/ERK may be the main pathway involved.
The E. coli-induced disease rat model of fallopian tube tissue showed damage, mitochondrial
disruption, and increased levels of the inflammatory factors IL-6 and TNF-α. Tubal inflammatory
infertility rats have increased expression of Bcl-2, p-ERK1/2, p-MEK, and p-EGFR, and
decreased expression of ESR1. In vivo, experiments showed that YJP improved damage of tissue,
inhibited shedding of tubal cilia, and suppressed the inflammatory response of the body. Furthermore,
YJP inhibited EGFR/MEK/ERK signaling, inhibited the apoptotic protein Bcl-2, and upregulated
ESR1.

Conclusion:

This study revealed that YJP Reducing tubal inflammation and promoting tissue repair
may be associated with inhibition of the EGFR/MEK/ERK signaling pathway.
TPD52 is a Potential Prognostic Biomarker and Correlated with Immune Infiltration: A Pan-cancer Analysis
Authors: Miao Lu , Jing Li , Chen Buze , Zeng Tian , Chen Youguo
Published: October 2023

Background:

Some tumors have a poor prognosis regarding TPD52 (tumor
protein D52). This study aims to explore TPD52's role in the cancer process from a
pan-cancer perspective.

Methods:

A pan-cancer analysis was conducted to investigate how TPD52 may be
involved in cancer as well as its association with prognosis.

Results:

A variety of human cancers express TPD52 abnormally and correlate with
clinical stage. There was a significant association between low expression of TPD52
and poor survival in BRCA, KIRP, LAML, LIHC, UCEC, and UVM. TPD52 alterations
were most frequently amplified in pan-cancer. The co-occurrence of 10 genes
alterations was found in the TPD52 altered group. There was a significant association
between TPD52 expression and MSI in four cancer types and TMB in twelve cancer
types. There was a significant correlation between TPD52 expression and immunerelated
cell infiltration. A significant correlation was found between TPD52 expression
in many tumor types and 8 immune checkpoint genes. There were signaling pathways
involved in pan-cancer caused by TPD52, including endocytosis, Fc gamma Rmediated
phagocytosis, and so on. TPD52 may be involved in chemotherapy and
chemoresistance.

Conclusion:

The TPD52 gene may be important for human cancer treatment.