bluuebunny/crossref_metadata_2025 huggingface dataset:
INSERÇÃO DAS TIC’S NO PROCESSO DE ENSINO APRENDIZAGEM NA PERSPECTIVA DO TRABALHO DOCENTE
Authors: Oliveira Jerffeson Miguel de , Moura Keila Dayane Pereira
Published: August 2020

This article presents a brief investigation on the real conditions about the needs and possibilities in the insertion of ICT's in the learning teaching process developed in schools of the public education network in the municipality of Piripiri, state of Piauí. This study is supported by the following objectives: to identify the difficulties and possibilities in the use of digital technologies as a didactic resource in elementary school classrooms I, in public schools in the municipality of Piripiri. Investigate the availability of technological resources for teaching professionals, reflect the importance of ICT's for the learning teaching process and analyze the main difficulties faced by teachers regarding the use of ICT's in the classroom. The methodology used is qualitatively oriented, the study carried out with data collection through field research, developed from the application of a questionnaire for teachers of municipal schools of Piripiri. From the analysis of the data collected during the research, it was noticed that the insertion of technologies in the learning teaching process comprises a long term path, requiring continuing education policies, which opportunism the teacher to acquire new skills and competencies, the creation of a set of actions aimed at strengthening the teacher relationship (a) / student (a). The conclusive aspect is that such policies and actions can make it possible to better know the public and the real conditions of practical applications for the use of ICT's in the classroom, considering the difficulties and possibilities expressed by teachers and the precarious conditions of equipment and laboratories present in schools. It showed how difficult the lack of equipment limits teaching work, however, transformative practice coexists with such difficulties when the teacher assumes the profile of researcher and an autonomous posture for the use of such resources.
A CIDADE SUBMARINA: UM EXERCÍCIO PROJETUAL INTERDISCIPLINAR
Authors: Beltrão André Luis Ferreira , Couto Rita Maria , Ribeiro Flavia Nizia Fonseca
Published: August 2020

This paper presents a pedagogical experiment of context shift applied to undergraduate Design classes, with students from the second period of the course. The proposal consisted of a project exercise that transported students to the bottom of the sea to participate in an interdisciplinary learning experience in the fictional context of a Submarine City, enabling the displacement of their creative references and enabling conditions that enhanced meaningful learning. The article describes the exploration of a way of teaching related to the search for innovative solutions to problems that can be extended to disciplines of any area of knowledge.
LETRAMENTO DIGITAL E FORMAÇÃO DE PROFESSORES: UMA PROPOSTA COM O APLICATIVO WHATSAPP
Authors: Marques Gersion Rodrigues , Sabota Barbra , Santclair Dllubia
Published: August 2020

This article aims to problematize the concept of digital literacy from experiences using Information and Communication Digital Technologies (ICDT) in a six-hour course planned for teacher education context. Six undergraduate students took part in this minicourse. Our intention is to provide opportunities for reflection on the opportunities and digital didactic resources of our time while providing that the discussions take place also mediated by these technologies. In this way, the participants of the minicourse were invited to take a meta-analytical look at the tool, that is, to use and reflect on how to use it. This qualitative interpretive study is guided by the theory of Multiliteracies and aims to discuss how we can contribute to the improvement of critical digital literacy of teachers and consequently favor work based on the theory of critical literacy resignified also among their students.
ANÁLISE DA EXISTÊNCIA E ABORDAGEM DA CONCEPÇÃO DE CTS EM LIVRO DIDÁTICO DE QUÍMICA
Authors: Mota Fabricio Gabriel , Comiotto Tatiana , Raulino Fernanda , Santos Patrícia Amynthas , Dias Lorena Silva de Andrade
Published: August 2020

This article presents, from the perspective of STS (Science, Technology and Society), one of the didactic resources most used by teachers: the textbook. The objective was to identify the conceptions present, regarding STS, in the textbooks of Chemistry for High School, belonging to the National Program of Didactic Book of the Ministry of Education. For that, a spreadsheet was created establishing criteria to be evaluated and a guide of definitions to increase the degree of impartiality of the analysis. Thus, this research opted for the collection adopted by the Federal Institute Catarinense (FIC), entitled "Chemistry", by the authors Mortimer and Machado, since the researchers worked at this institution and adopted this teaching material. After evaluation, through the proposed instrument, it was observed that the collection presents some shortcomings in its organization and contextualization - often exemplifying instead of contextualizing -, proposing closed activities and not relating them to everyday life. Therefore, it is identified that the work is relevant to its approach, but presents space for improvements regarding the items mentioned.
Study of the remediation effects of passivation materials on Pb-contaminated soil
Authors: Liang Shu-Xuan , Xi Xiao-Can , Li Yu-Ru
Published: August 2020

The passivation effects of blast furnace slag, fly ash, corncob biochar, and phosphate fertilizer in Pb-contaminated soil was evaluated against the soil pH, available Pb content, Pb fractions, and bioactivity coefficient. Blast furnace slag and fly ash could increase soil pH, while corncob biochar and phosphate fertilizers lowered soil pH. The available Pb content in the blast furnace slag and phosphate fertilizer treatment groups was significantly lower than in other treatments. Also, blast furnace slag and phosphate fertilizer could significantly convert nonresidual Pb into residual Pb. Combined with the environmental impact after application and cost of the material, it is recommended that blast furnace slag can be used as a passivation agent for low-concentration Pb-contaminated soil.
Stability-indicating HPLC-DAD assay for simultaneous quantification of hydrocortisone 21 acetate, dexamethasone, and fluocinolone acetonide in cosmetics
Authors: Arif Saira , Ata Sadia
Published: August 2020

A rapid and specific method was developed for simultaneous quantification of hydrocortisone 21 acetate (HCA), dexamethasone (DEX), and fluocinolone acetonide (FCA) in whitening cream formulations using reversed-phase high-performance liquid chromatography. The effect of the composition of the mobile phase, analysis temperature, and detection wavelength was investigated to optimize the separation of studied components. The analytes were finally well separated using ACE Excel 2, C18 AR column having 150 mm length, 3 mm internal diameter, and 2 µm particle size at 35°C using methanol with 1% formic acid and double-distilled deionized water in the ratio of 60:40 (v/v), respectively, as the mobile phase in isocratic mode. Ten microliters of sample were injected with a flow rate of 0.5 mL/min. The specificity, linearity, accuracy, precision, recovery, limit of detection (LOD), limit of quantification (LOQ), and robustness were determined to validate the method as per International Conference on Harmonization guidelines. All the analytes were simultaneously separated within 8 min, and observed retention times of HCA, DEX, and FCA were 4.5, 5.5, and 6.9 min, respectively. The proposed method showed good linearity with the correlation coefficient, R2 = 0.999 over the range of 1–150 µg/mL for all standards. The linear regression equations were y = 12.7x + 118.7 (r = 0.999) for HCA, y = 12.9x + 106.8 (r = 0.999) for DEX, and y = 12.9x + 96.8 (r = 0.999) for FCA. The LOD was 0.25, 0.20, and 0.08 µg/mL for HCA, FCA, and DEX and LOQ was 2.06, 1.83, and 1.55 µg/mL for HCA, FCA, and DEX, respectively. The recovery values of HCA, DEX, and FCA ranged from 100.7–101.3, 102.0–102.6, and 100.2–102.0%, respectively, and the relative standard deviation for precision (intra- and interday) was less than 2, which indicated repeatability and reproducibility. The novelty of the method was described by forced degradation experimentation of all analytes in the combined form under acidic, basic, oxidative, and thermal stress. The proposed method was found to be simple, rapid, and reliable for the simultaneous determination of HCA, DEX, and FCA in cosmetics.
Design, synthesis and pharmacological profile of (−)-verbenone hydrazones
Authors: Nesterkina Mariia , Barbalat Dmytro , Kravchenko Iryna
Published: August 2020

A series of novel (−)-verbenone hydrazones was designed and synthesized via condensation of terpenoid with hydrazides derived from phenoxyacetic acid. The structure of target compounds was confirmed by 1H-NMR and 13C-NMR analysis, Raman and FT-IR spectroscopy, electrospray ionization method and fast atom bombardment (FAB) mass spectrometry. Thermal properties of (−)-verbenone hydrazones 3a–3e were estimated by differential scanning calorimetry and their purity by HPLC coupled to mass spectrometry. Verbenone hydrazones were revealed to exist as Z/E geometrical isomers about C═N bond and cis/trans amide conformers. Verbenone derivatives were estimated as potential anticonvulsant agents after their oral administration against pentylenetetrazole and maximal electroshock-induced seizures in mice. Analgesic effect of hydrazones was studied by topical application on models of allyl isothiocyanate and capsaicin-induced pain. The present findings indicate that verbenone hydrazones contribute to seizure protection both at short (6 h) and long (24 h) time periods by blocking chemical- and electroshock-induced convulsions. Binding of compounds 3a–3e to TRPA1/TRPV1 ion channels was suggested as a feasible mechanism explaining their significant analgesic activity.
PCSK9 concentrations in different stages of subclinical atherosclerosis and their relationship with inflammation
Authors: Tóth Štefan , Olexa Peter , Hertelyová Zdenka , Štefanič Peter , Kopolovets Ivan , Berek Peter , Filip Vladimir , Chakravarty Ryan , Široká Monika , Pella Daniel
Published: August 2020

The aim of this study was to detect the concentrations of PCSK9 in various subclinical stages of atherosclerosis and to highlight its relationship with inflammation. One hundred and fifty-nine healthy patients were divided into three groups, based on the extent of atherosclerotic changes in the carotid artery: a group without identifiable atherosclerosis, cIMT>75‰and an asymptomatic plaque group. The PCSK9 was measured by ELISA and hsCRP by the immunoturbidimetric method. Vascular changes were identified by a carotid ultrasound. PCSK9 was elevated, when comparing the healthy group with the cIMT>75‰group; however, no significant increase was detected between cIMT>75‰and the asymptomatic plaque group. A positive linear correlation of the PCSK9 concentration and atherosclerotic changes was found; however, after the re-analysis in each group, this correlation persisted only in the group with still normal values. Additionally, a significant linear correlation was found between the PCSK9 concentrations and lipid parameters. However, no significant association was found with hsCRP. PCSK9 was found to be elevated only in cIMT>75‰, but not in the later plaque stage. A linear correlation of PCSK9 values was detected only in the group with still reference values. Based on this fact, we assumed the direct linear role of PCSK9 in initiating atherosclerosis; however, in the later phases, the relationship, which highlights other risk factors such as inflammation, is not linear.
New derivatives of a natural nordentatin
Authors: Thant Tin Myo , Aminah Nanik Siti , Kristanti Alfinda Novi , Ramadhan Rico , Aung Hnin Thanda , Takaya Yoshiaki
Published: August 2020

New derivatives were obtained from natural nordentatin (1) previously isolated from the methanol fraction of Clausena excavata by an acylation method. Herein, we report ten new pyranocoumarin derivatives 1a–1j. Their structures were elucidated based on UV-vis, FT-IR, NMR, and DART-MS data. The α-glucosidase inhibition and anticancer activities of nordentatin (1) and its derivatives were also evaluated. The α-glucosidase inhibition assay exhibited that the derivatives 1b, 1d, 1e, 1f, 1h, 1i, and 1j possess higher inhibitory activity for α-glucosidase with IC50 values of 1.54, 9.05, 4.87, 20.25, 12.34, 5.67, and 2.43 mM, whereas acarbose was used as the positive control, IC50 = 7.57 mM. All derivatives exhibited a weak cytotoxicity against a cervical cancer (HeLa) cell line with the IC50 between 0.25 and 1.25 mM. They also showed moderate to low growth inhibition of a breast cancer (T47D) cell line with IC50 values between 0.043 and 1.5 mM, but their activity was lower than that of the parent compound, nordentatin (1) (IC50 = 0.041 mM).
Estrategia de gestión para la generación de recursos en un centro de investigación y posgrado. Estudio de caso del CIITEC en el contexto de México
Authors: Peinado Camacho José de Jesús , Montoy Hernández Luis Daniel , Torres Hernández Zacarías
Published: August 2020

Cuando las políticas públicas son de austeridad, los recursos económicos disminuyen y las oportunidades son escasas. En condiciones como esta es momento de pensar, sugerir y aplicar nuevas alternativas para superar situaciones adversas. En este contexto, el objetivo de la investigación es proponer una estrategia para la generación de recursos económicos a través de pruebas de laboratorios acreditados para el Centro de Investigación e Innovación Tecnológica (Ciitec). El diseño de la investigación es no experimental. El tipo de estudio es descriptivo, y como técnica de investigación se utilizó el estudio de caso. La presente investigación aportó información útil sobre las pruebas de laboratorios acreditados, por lo que se puede tener una administración eficiente a fin de elevar los ingresos monetarios. Entre los resultados relevantes se encontró que el Ciitec tiene infraestructura de servicios, capacidad instalada, capital intelectual y gestión del conocimiento; por esta razón, tiene mayores ventajas y mejores elementos para generar recursos.
Can Foot Anthropometry Predict Vertical Jump Performance?
Authors: Hawley Victoria S. , Gurchiek Reed D. , van Werkhoven Herman
Published: August 2020

Hawley, VS, Gurchiek, RD, and van Werkhoven, H. Can foot anthropometry predict vertical jump performance? J Strength Cond Res 36(7): 1860–1865, 2022—Vertical jumping is an important element of many sporting activities, and whether anthropometric adaptations can predict jumping performance is of interest. Few studies have specifically considered anthropometric measures of the foot and its link to performance. Furthermore, previous studies have mainly focused on a male subject pool, and whether relationships are consistent across sexes is unclear. The purpose of this study was to investigate relationships between common anthropometric measures, as well as specific foot measures, and jump performance in men and women. Anthropometric measures of 21 men (age: 22.0 ± 1.5 years; stature: 181.4 ± 6.3 cm; body mass: 85.6 ± 9.4 kg) and 21 women (age: 21.2 ± 1.8 years; stature: 166.1 ± 7.5 cm; body mass: 61.4 ± 11.4 kg) were taken before performing 3 maximal countermovement jumps (CMJs). Correlational analysis was used to determine relationships between anthropometric measures and CMJ height (a priori significance set at p≤ 0.05, effect size: small >0.1; medium >0.3; large >0.5). There was no significant correlation between anthropometric variables and CMJ height for men, whereas for women, mass (r = −0.585, p = 0.005, large effect), foot length (r = −0.533, p = 0.013, large effect), and toe length (r = −0.604, p = 0.004, large effect) showed significant negative correlations with CMJ height. The unexpected result that smaller feet and toes predicted higher jumps for women warrants further investigation. Furthermore, these results highlight the need to incorporate diverse subject pools, and a need for caution when generalizing across sexes.
Astrocytes Function as an Intermediate for Retrograde Endocannabinoid Signaling in the Suprachiasmatic Nucleus to Influence Circadian Clock Timing
Authors: Carney Rosalind S.E.
Published: August 2020

Highlighted Research Paper:Cannabinoid Signaling Recruits Astrocytes to Modulate Presynaptic Function in the Suprachiasmatic Nucleus. Lauren M. Hablitz, Ali N. Gunesch, Olga Cravetchi, Michael Moldavan and Charles N. Allen.
Tritium measurement using a photo-stimulable phosphor BaFBr(I):Eu2+ plate
Authors: Ohuchi H. , Hatano Y.
Published: August 2020

Tritium measurement is indispensable for the fuel- processing systems of deuterium-tritium (DT)-fusion facilities. A new approach to detect tritium in regions deeper than the escape depth of beta-rays from tritium is being developed using an imaging plate (IP). The measurement principle of this approach is to observe bremsstrahlung X-rays induced by the tritium beta-rays. An IP made of europium-doped BaFBr(I), a photostimulated luminescence (PSL) material, is a two-dimensional radiation sensor. In the present study, the characteristics of this IP for measuring tritium by detecting bremsstrahlung X-rays, in particular a fading effect and the energy dependence of PSL sensitivities, are examined.
Lanthanide and actinide extractions with cobalt bis(dicarbollide) ion derivatives covalently bonded to diglycolyl diamide platform
Authors: Lučaníková M. , Selucký P. , Rais J. , Grüner B. , Kvíčalová M.
Published: August 2020

The separation of minor actinides from high-level waste (HLW) remains a difficult problem. A wide series of compounds based on two cobalt bis(dicarbollide) ions [(1,2-C2B9H10)(1′,2′-C2B9H11)-3,3′-Co(III)]− (1) covalently bonded to a diglycolyl amide platform via diethyleneglycol connectors was prepared by reaction of the diglycolic acid dichloride with various ammonium derivatives of the ion 1 with the aim to develop new class of extractants for trivalent radionuclides from acidic waste solutions. The extractants of general formulation [{(N,N-(8-CH2-CH2O)2-1,2-C2B9H10)(1′,2′-C2B9H11)-3,3′-Co)(N,N′-R)NCOCH2}2-O]Na2 differed in substitution on the amidic nitrogen (by e.g. butyl-, hexyl-, octyl-, t-octyl-, dodecyl-, benzyl-groups). Compounds were characterized by combination of 11B, 1H and 13C NMR spectroscopy, ESI-MS, HPLC, and TLC RF. The purity of all species was better than 98%. Most of the compounds proved to be very efficient extraction agents and enabled effective extraction of europium(III) and americium(III) from nitric acid solutions. The recovery of the trivalent radionuclides from the loaded organic phase was possible by using ammonium citrate or even better by using solution of ammonium citrate and ammonium DTPA (diethylenetriamine pentaacetate).
Separation of Am(III), Cm(III), and Cf(III) using capillary electrophoresis
Authors: Kikunaga H. , Yoshimura T. , Kuribayashi T. , Kitamoto Y. , Takahashi N. , Haba H. , Ezaki Y. , Enomoto S. , Mitsugashira T. , Shinohara A.
Published: August 2020

Trivalent actinides Am(III), Cm(III), and Cf(III) were successfully separated for the first time using capillary electrophoresis in 2-hydroxyisobutyric acid/acetic acid. It was found that the ionic radius was primarily important for separation of trivalent actinides as well as lanthanides in this condition. The stability constants of the Am(III) complexes with 2-hydroxyisobutyrate were estimated using the correlations between the molar fraction ratio of lanthanides and their ionic radii.
Rapid separation and extraction of radioactive analytes onto filters and surfaces
Authors: Gonzáles E. R. , Klingensmith A. L. , Peterson D. S.
Published: August 2020

This paper presents a radiochemical procedure that combines radiochemical sample preparation and analyte separation to provide a rapid sample preparation method. Extractive ligands are prepared in a polymer matrix. This system can be immobilized on a surface or on a filter. Filters allow rapid sample uptake due to the movement of the analyte through the filter material. These approaches offer fast uptake kinetics, high material loading, and good selectivity. These systems have been demonstrated for the rapid extraction of plutonium and americium from solution and their subsequent analysis by alpha spectrometry, with no additional sample preparation required, thus eliminating extensive wet chemical processes that would otherwise be necessary. We have evaluated the extraction efficiency that can be obtained and have shown that these systems can provide for alpha spectrometry peak widths near what can be obtained using traditional techniques such as electroplating. We have also demonstrated Polymer Ligand Films (PLFs) for the selective extraction of plutonium from a solution prepared from a soil digest.
Preparing a laboratory for radioanalytical emergency response
Authors: Bennett J. , Webb C. J. , Isch S.
Published: August 2020

As the state of the nation's ability to respond to a radiological event is examined, it has become apparent that both capacity and capability are lacking. Department of Homeland Security National Planning Scenario #11 is designed to address the planning activities for the response to an attack using radiological dispersal devices. The scenario details show that the cleanup activity will take several years, and that there will be between 360000 and 1000000 environmental samples in the first year. Based on existing capacity and capabilities it would take four to six years to analyze the samples generated at the lower end of the sample range.

The Environmental Protection Agency (EPA) has been given responsibility for the remediation activities following a radiological event, and has awarded cooperative agreements to several laboratories to start the process of developing capacity and capabilities. The Connecticut Department of Public Health Laboratory (DPHL) was awarded one of the cooperative agreements. The DPHL has started activities to further those goals by investigating and implementing procedures to ensure that samples with activity higher than normal background can be processed safely, as well as implementing more rapid methods for radiochemical analysis. The DPHL already served as the primacy radiochemistry laboratory for several New England states and thus had a solid foundation to build upon. The DPHL has taken a process flow approach in preparing for radiological emergency response and recommends that radioanalytical laboratories that are reviewing their roles in such a response:

• Ensure that their Nuclear Regulatory Commission licenses allow for appropriate radioisotope types and activities;

• Develop procedures and processes to ensure that samples with higher activities can be processed safely, with due regard for sample screening and aliquanting samples;

• Provide for enhanced radioanalytical contamination control, with careful consideration of sample flow and breaking the laboratory into zones with controlled access;

• Address personnel safety by enhancing training, adding real time dosimetry to exposure monitoring protocols, and reviewing personal protective equipment and hygiene protocols with staff;

• Develop plans for spills and decontamination, as well as for increased monitoring of laboratory areas;

• Plan for secure sample storage;

• Exercise the plan.
Nuclear field shift effect of lead in ligand exchange reaction using a crown ether
Authors: Fujii T. , Moynier F. , Agranier A. , Ponzevera E. , Abe M.
Published: August 2020

Lead isotopes were fractionated by the liquid-liquid extraction technique between an aqueous phase and a crown ether. After purification by ion-exchange chemistry, the 207Pb/206Pb and 208Pb/206Pb isotopic ratios were measured by multiple-collector inductively coupled plasma mass spectrometry (MC-ICP-MS). Isotope fractionations > 0.05‰ have been found. The conventional equilibrium mass-dependent isotope effect estimated by an ab initio calculation was smaller than the Pb isotope fractionation experimentally obtained. Conventional mass-dependent isotope fractionation is not a valuable explanation for our results. The nuclear field shift effect, which results from the isotopic change in the nuclear size and shape, was also estimated by an ab initio method combined with a finite nucleus model. The nuclear field shift effect calculated was in agreement with our experimental results. Therefore, this study reports the first nuclear field shift effect of Pb in an equilibrium ligand exchange reaction.
The Potential of Using Grapefruit Peel as a Natural Support for Yeast Immobilization During Beer Fermentation
Authors: Estela-Escalante Waldir D. , Perez-Escalante Jimy J. , Fuentes-Navarro Eduardo L. , Pinillos-Miñano Ricardo M.
Published: August 2020

The potential use of grapefruit peel as support material for yeast immobilization during beer fermentation was evaluated. After conditioning, FTIR analysis revealed a higher quantity of methoxy (–OCH3) groups, suggesting that lignin is the major component of the support. Cell adhesion onto the conditioned support in 12°Plato laboratory malt wort was evaluated, observing a maximal cell adhesion (2.25 · 109 cells/gram of dried support) at 20 h of cultivation, remaining almost constant in the subsequent time points. Evaluations of the fermentative behaviour of the biocatalyst at 15±0.5 °C in a 14°Plato laboratory malt wort indicated good stability in terms of physical integrity (confirmed by SEM observation). The fermentation time was shortened to four days, and the rates of reducing sugar consumption and ethanol production were improved when compared to fermentations carried out with free suspended cells. These results show a promising potential of grapefruit peel as support material in beer fermentation.
Paspalum notatum Grass-waste-based Adsorbent for Rhodamine B Removal from Polluted Water
Authors: Zahir Abdul , Aslam H.M. Zaheer , Aslam Umair , Abdullah Ahmed , Ali Rizwan , Bello Mustapha Mohammed
Published: August 2020

The potential of Paspalum notatum grass waste to adsorb Rhodamine B dye from aqueous phase is reported in this research. The grass waste was activated and characterized through various techniques to analyze the chemical (FTIR), morphological (SEMEDX), and thermal (TGA) changes incorporated through the activation process. The pollutant removal efficiency of the raw and modified adsorbents was studied by varying different process parameters in a batch process. The maximum capacity of adsorption which was observed for grass waste and activated grass waste was 54 mg g–1 and 72.4 mg g–1 respectively. Among the various kinetic models, the pseudo-second order model gives the best regression results. However, the intraparticle diffusion-adsorption model showed that the diffusion within pores controlled the adsorption rate. Thermodynamic analysis of this process revealed that Rhodamine B adsorption was endothermic and spontaneous in nature. The results of this study show that grass waste has the potential to be used as an adsorbent for the treatment of colored water.
Hybrid Genome Assembly and Evidence-Based Annotation of the Egg Parasitoid and Biological Control AgentTrichogramma brassicae
Authors: Ferguson Kim B , Kursch-Metz Tore , Verhulst Eveline C , Pannebakker Bart A
Published: August 2020

Trichogramma brassicae (Bezdenko) are egg parasitoids that are used throughout the world as biological control agents and in laboratories as model species. Despite this ubiquity, few genetic resources exist beyond COI, ITS2, and RAPD markers. Aided by a Wolbachia infection, a wild-caught strain from Germany was reared for low heterozygosity and sequenced in a hybrid de novo strategy, after which several assembling strategies were evaluated. The best assembly, derived from a DBG2OLC-based pipeline, yielded a genome of 235 Mbp made up of 1,572 contigs with an N50 of 556,663 bp. Following a rigorous ab initio-, homology-, and evidence-based annotation, 16,905 genes were annotated and functionally described. As an example of the utility of the genome, a simple ortholog cluster analysis was performed with sister species T. pretiosum, revealing over 6000 shared clusters and under 400 clusters unique to each species. The genome and transcriptome presented here provides an essential resource for comparative genomics of the commercially relevant genus Trichogramma, but also for research into molecular evolution, ecology, and breeding of T. brassicae.
Evaluation of physicochemical properties and dissolution studies on quality control of low water solubility drugs (raw materials and pharmaceutical formulations)
Authors: Da Silva Ferreira Matheus , De Carvalho Teles Júnior Gilmar Antônio , Ramos Carvalho Júnior Carlos Magno , De Souza Dias Fernanda , Dias dos Santos Júnior Wilson Saback , Oliveira da Guarda Souza Marluce , De Freitas Santos Júnior Aníbal
Published: August 2020

The purpose of this study was to evaluate physicochemical properties and dissolution studies of furosemide (FUR), hydrochlorothiazide (HCTZ) and nifedipine (NIF), low water solubility drugs, in raw materials and pharmaceutical formulations. Surface and physicochemical characterization techniques -scanning electronic microscopy (SEM), thermogravimetry (TG), X-ray diffraction (XRD) and infrared (IR) spectrometry- as well as physical and physicochemical tests on tablets and capsules were applied as supporting information on drug quality control. Simple, rapid, and efficient UV-Vis methods were developed and validated for the determination of FUR, HCTZ and NIF samples. SEM exhibited considerable differences in the crystal morphological structures. Among the drugs studied, except for furosemide, more than one polymorph was present in the samples. Drug release profiles were satisfactory for all products. FUR and HCTZ tablets exhibited similar dissolution profiles, with very rapid release to the pharmaceutical specialties (reference, similar and generic). For HCTZ tablets, the similar drug (f2= 48.74) is not equivalent to the reference drug. NIF capsules (reference and compounded) showed a release ≥80% of stated on product labels, in 10 minutes. The results obtained in this study suggest that the quality parameters and drug dissolution profiles may have been influenced by the morphology and size of the crystals, excipients, and technological processes.
Evaluación de la publicidad de medicamentos en los principales medios televisivos, radiofónicos y escritos en Costa Rica
Authors: Corrales-Cubillo Yenifer , Saborío-Quesada Nathalie , Alfaro-Mora Ramsés
Published: August 2020

La presente investigación evaluó la publicidad de medicamentos en los principales medios televisivos, radiofónicos y escritos en Costa Rica para conocer la información que se transmite al consumidor. Los medios de comunicación son interesantes objetos de estudios porque son usados por las industrias como una herramienta para poder llegar al consumidor. Este es un estudio observacional, descriptivo de tipo transversal, se realizó una encuesta a la población estudiantil de la Universidad Latina de Costa Rica y con la misma se hizo una observación de las pautas publicitarias en la radio, periódicos y la televisión en diferentes horarios. En la investigación se determinó que la mayoría de los anuncios encontrados en los diferentes medios de comunicación carecieron de ciertas disposiciones solicitadas por el reglamento. Las faltas que se encontraron fueron: composición del producto (principios activos), casa fabricante, categoría del producto, presentaciones disponibles. Se demostró que las regulaciones en la parte de la promoción de un medicamento son de gran importancia para poder proteger a los consumidores, porque las ganancias de las industrias farmacéuticas dependen en gran medida de su mercadotecnia. La promoción de productos busca aumentar las ganancias, sin embargo, si esto no se hace correctamente podría haber una mala utilización de los medicamentos.
A descrição matemática da detecção eletroquímica dos alcaloides do grupo da insubosina, assistida pelos novos triazóis com e sem composto de ligação
Authors: Tkach Volodymyr V. , Kushnir Marta V. , De Oliveira Sílvio C. , Parchenko Volodymyr V. , Odyntsova Vira M. , Aksyonova Ilona I. , Luganska Olga V. , Korne Maryna M. , Ivanushko Yana G. , Yagodynets´ Petro I.
Published: August 2020

Pela primeira vez, foi feita uma abordagem teórica do desempenho dos novos derivados triazólicos durante a determinação química dos alcaloides acridínicos do grupo insubosina, na presença de uma concentração pequena de um composto de ligação. A detecção eletroanalítica é realizada mediante um mecanismo híbrido, envolvendo ou não a formação de novos compostos iônicos. É possível mostrar que o estado estacionário é estabilzado, apesar de a região topológica, que lhe é correspondente, ser mais estreita que no caso da detecção mais comum. O comportamento oscilatório, neste caso, é mais provável que nos casos mais comuns, mas o fator adicional é relacionado apenas com a introdução de um composto de ligação.
Forced degradation studies and stability-indicating liquid chromatography method for determination of tirofiban hydrochloride and synthetic impurities
Authors: Roll Feijó Adriane Lettnin , Macke Hellwig Fernanda , Soldateli Paim Clésio , Malesuik Marcelo Donadel
Published: August 2020

This study aimed to develop and validate a stability-indicating liquid chromatography method for the determination of tirofiban hydrochloride and two synthetic impurities (impurity A and impurity C). The method utilizes a RP-18 column (250 mm × 4.6 mm; 5 μm) with the PDA detector for quantitation. A mixture of triethylamine 0.1% (acidified to pH 5.5 with phosphoric acid) and acetonitrile was used as the mobile phase at a flow rate of 1 mL min−1 with gradient elution. The method presented satisfactory linearity, precision, accuracy and robustness, as well as low limits of detection and quantification, which demonstrate sensitivity in the determination of tirofiban and impurities A and C. It was selective for the determination of the drug and impurities analysed, without interference of the degradation products generated under forced conditions, demonstrating the stability-indicating capacity of the proposed method. Tirofiban showed to be practically stable to oxidative (30% H2O2 for 24 h) and thermal (75 ºC for 24 h) conditions, but presented degradation to UVA light and acid hydrolysis, obeying the first order kinetics for both. In this way, it can be used as a stability-indicating method in the quality control of the raw material of tirofiban hydrochloride, as well as of the finished product. The obtained results demonstrate the importance of deepening the studies in this area, in order to guarantee the quality of commercialized pharmaceutical products.
Optimal Detection of Bilinear Dependence in Short Panels of Regression Data
Authors: Lmakri Aziz , Akharif Abdelhadi , Mellouk Amal
Published: August 2020

In this paper, we propose parametric and nonparametric locally andasymptotically optimal tests for regression models with superdiagonal bilinear time series errors in short panel data (large n, small T). We establish a local asymptotic normality property– with respect to intercept μ, regression coefficient β, the scale parameter σ of the error, and the parameter b of panel superdiagonal bilinear model (which is the parameter of interest)– for a given density f1 of the error terms. Rank-based versions of optimal parametric tests are provided. This result, which allows, by Hájek’s representation theorem, the construction of locally asymptotically optimal rank-based tests for the null hypothesis b = 0 (absence of panel superdiagonal bilinear model). These tests –at specified innovation densities f1– are optimal (most stringent), but remain valid under any actual underlying density. From contiguity, we obtain the limiting distribution of our test statistics under the null and local sequences of alternatives. The asymptotic relative efficiencies, with respect to the pseudo-Gaussian parametric tests, are derived. A Monte Carlo study confirms the good performance of the proposed tests.
Efecto de caracteres propios e inherentes al animal sobre la edad al primer parto en la hembra bovina de la raza Romosinuano
Authors: López Martínez Jorge Leonardo , Quijano Bernal Jorge Humberto , Garcés Blanquicet Jorge Luis , Ossa Sarraz Gustavo Alfonso
Published: August 2020

El objetivo del trabajo consistió en caracterizar fenotípica y genéticamente la edad al primer parto de hembras bovinas de la raza Romosinuano del banco de Germoplasma Bovino del Centro de Investigación Turipaná. Para esto, se usaron 1190 registros de edad al primer parto, obtenidos entre los años de 1936 al 2015. Inicialmente, un bootstrapping se llevó a cabo para determinar que variables tenían efecto sobre dicho carácter. Posteriormente, los parámetros genéticos para el mismo, fueron obtenidos previa estimación de los componentes de varianza a partir del modelo animal, mediante máxima verosimilitud restringida, utilizando el programa MTDFREML. La media y desviación para la edad al primer parto fue de 34.56 ± 3.48 meses y el índice de herencia de 0.32 ± 0.09. Se evaluaron los efectos fijos de sexo de la cría, y las clases de año y mes de nacimiento de la hembra bovina, de los cuales las dos últimas tuvieron influencia significativa sobre el carácter. En general, el índice de herencia indicó que la selección para la edad al primer parto puede tener un efecto interesante para promover cambios deseables en la composición genética, en caso de que se desease promover ganancias genéticas para este carácter en la población bovina de la raza Romosinuano.
Helicobacter pylori y la expresión de genes en pacientes con adenocarcinoma gástrico
Authors: Rabanal-Sanchez Jhonatan
Published: August 2020

El cáncer gástrico (CG) es uno de los canceres con mayor incidencia en el mundo. Diversos estudios han asociado la presencia de una infección crónica por Helicobacter pylori, como uno de los procesos claves en la formación, crecimiento y desarrollo de este cáncer. El adenocarcinoma gástrico es un subtipo de CG y representa alrededor del 90% de los casos reportados. Muchos estudios en pacientes con CG, han reportado la presencia de mutaciones en genes que cumplen funciones importantes en señalización celular, integridad genómica, adhesión celular, remodelación de cromatina, motilidad celular y formación del citoesqueleto. En este trabajo se analizó los datos provenientes del atlas del genoma del cáncer(TCGA), del cual se obtuvo datos de la expresión de estos genes (expresados en transcripciones por millón); se obtuvo la mediana, valor de los cuartiles y el ρ-valor de la diferencia de medianas. Se encontró que la mayor parte estaban sobreexpresados en pacientes con adenocarcinoma gástrico. Sin embargo, algunos genes presentaron una expresión similar a lo observado en condiciones no cancerosas, cuando estaba presente una infección por H. pylori; y estos mismos genes presentaron una sobreexpresión en ausencia de H. pylori. Este estudio plantea que estos resultados se deberían a la metilación del ADN producida por esta bacteria, por lo que este mecanismo inhibiría la expresión de estos genes.
Implementación de un Proyecto Pedagógico Productivo (PPP) en el marco de la piscicultura
Authors: Candela Boris , Salazar Jenny Viviana
Published: August 2020

La enseñanza de la química en la escuela secundaria se ha llevado a cabo desde una perspectiva descontextualizada y focalizada en los contenidos y el profesor, situación que limita la oportunidad a los estudiantes de comprometerse en la construcción de los productos y procesos de la química. Con el ánimo de superar estas restricciones, este estudio se propuso implementar un PPP representado en la piscicultura a fin de asistir a los estudiantes de   básica secundaria ciclo II rural, en la comprensión del tópico de las disoluciones junto con el desarrollo de algunas habilidades para la vida. La metodología fue cualitativa e interpretativa por estudios de casos. Esta estuvo configurada por dos fases: planeación e implementación de una secuencia de actividades de aprendizaje, y documentación de los razonamientos y acciones de los estudiantes y el profesor. Los datos provenientes de las diferentes fuentes documentales fueron analizados desde la teoría fundamentada, emergiendo una serie de generalizaciones naturalisticas sustentadas por la afirmación que los PPP de la cría de trucha Arcoíris es una orientación de enseñanza contextualizada, la cual ayudó a los estudiantes a construir no sólo la comprensión del fenómeno de las disoluciones, sino, también, el desarrollo de habilidades como aprender a aprender y el trabajo cooperativo con el objetivo de informar el cultivo de esta clase de peces.
Efecto de la vigilancia Entomológica en la estructuración poblacional de triatoma infestans (hemiptera, reduviidae) de intra y peridomicilio en un área endémica de la Provincia de la Rioja (Argentina).
Authors: Hernández María Laura
Published: August 2020

Se analiza aquí, la influencia del rociado y vigilancia sobre la estructuración de las poblaciones peri e intradomésticas de T. infestans   usando morfología cuantitativa. Las colectas  en intra (ID) y peridomicilio (PD) se realizaron sobre una población libre de rociado por 10 años y en poblaciones hasta 8 años post rociado (población con vigilancia y rociado). En pre-rociado, no se observó estructuración poblacional entre ID y PDPost intervención,  se detectaron 6 adultos de T. infestans en  ID. Estos insectos mostraron  similitud morfológica con T. infestans residuales. Los resultados plantean que en presencia de vigilancia entomológica, los ID  pueden permanecer libres de T. infestans aún con PD infestados.
Discursos criminalizáveis: proposta de conceituação a partir de cartas de ameaça
Authors: Silva Welton Pereira e
Published: August 2020

Baseados em estudiosos do discurso (CHARAUDEAU, 2010, 2012; BARROS, 2011), bem como em estudos da Linguística Forense (SHUY, 2005) e do Direito Penal (BITENCOURT, 2010), analisamos duas cartas de ameaça com motivação homofóbica, procurando vislumbrar os imaginários sociodiscursivos que embasam a argumentação intolerante desenvolvida pelos sujeitos enunciadores. Notamos que, à condição homossexual, os sujeitos enunciadores das ameaças associam condutas imorais, orgias, brigas, drogas, bem como a bestialidade, i.e., a aproximação da relação homoafetiva ao animal, ao inumano. Além disso, emergem das cartas conflitos identitários que se assentam sobre a condição homossexual como oposta à identidade de “pessoas de bem” ou “servo de Deus”, em um posicionamento explicitamente contrário à comunidade LGBTQI+. Por configurarem discursos de ódio e ameaças, tais discursos podem ser tipificados como crime e, por isso, propomos, neste trabalho, a nomenclatura de discursos criminalizáveis como possíveis objetos de pesquisa na Linguística Forense.
Encenação e ubiquidade no Twitter: a intolerância dos discursos sobre Marielle Franco
Authors: Boaventura Luis Henrique , De Freitas Ernani Cesar
Published: August 2020

Este artigo trata da encenação do ato de linguagem em interações verbais na rede social Twitter. O objetivo geral é analisar o funcionamento do ato de linguagem na era da hipermobilidade sobre um clima político bipartidário e polarizado em que os parceiros de linguagem abdicam do contrato de comunicação a fim de fortalecerem sua posição original junto a seus pares do discurso político, rejeitando engajar uma negociação legítima de sentido com o lado oposto. O marco teórico se situa sobre a Teoria Semiolinguística, de Patrick Charaudeau (2010), e a comunicação ubíqua de Santaella (2013). A análise recai sobre uma troca linguageira ocorrida no Twitter acerca do assassinato de Marielle Franco e Anderson Gomes, em 14 de março de 2018. Concluímos que as partes opostas implementam a encenação do discurso a fim de conservar suas posições iniciais, o que as impede de considerar pontos de vista dissidentes.
“Comunicação não-violenta” pelas lentes da Linguística: embates no combate à intolerância
Authors: Motta Ana Raquel
Published: August 2020

O presente artigo visa a analisar e discutir a proposta conhecida como “Comunicação não-violenta” (CNV) através das lentes da Linguística. Tal proposta tem sido utilizada em muitos países do mundo como ferramenta no combate ao discurso intolerante, mas é pouco conhecida, ou até desprezada, nos meios acadêmicos dos Estudos da Linguagem. Este artigo analisa os quatro componentes da CNV: “fato/observação”, “sentimento”, “necessidade” e “pedido”, de acordo com o postulado em Rosenberg (2006, 2019). As conclusões são que a contribuição teórica da Linguística, especialmente de conceitos como “discurso”, “simulacro”, “evidencialidade”, “princípio da cooperação conversacional”, entre outros, proporciona mais clareza e coerência para noções utilizadas sem rigor teórico pela CNV e podem, por isso, contribuir para o desenvolvimento da proposta. Em relação à Linguística, espera-se que o artigo contribua para que a disciplina esteja mais engajada na construção de um mundo menos violento, seja analisando propostas já existentes de linguagem para a paz, como o que se faz aqui, seja propondo novas abordagens.
Documentos jurídicos, “cura gay” e legitimação do heterossexismo: uma análise discursiva do PDC 234/2011
Authors: Villela Gabriel Merlim Morae , Giorgi Maria Cristina , De Almeida Fabio Sampaio , Vargens Dayala Paiva de Medeiros
Published: August 2020

Este artigo, desdobramento de pesquisa realizada pelos grupos Práticas Discursivas na produção de Identidades Sociais: Fatores humanos, organizações, trabalho tecnologia e sociedade (PRADISIS) e Discurso e Educação Linguística (DELIN), tem como objetivo analisar documentos jurídicos, considerando sua suposta neutralidade (GONDIM, 2014), para entender como esses, a partir de determinadas relações de poder-saber (FOUCAULT, 2013), podem ser dispositivos de legitimação de práticas sociais de caráter heterossexista, que fundamentam discursos de ódio e intolerância, apontando para um caráter de intervenção na realidade social por parte da linguagem, e não somente de representação (ROCHA, 2006). Para isso, partiu-se do conceito de dialogismo (BAKHTIN, 2014), a partir do qual se compreende que os discursos são construídos por diversas vozes anteriores e posteriores, com as quais dialogam; uma abordagem da Análise do Discurso, de base enunciativa (MAINGUENEAU, 2004), que compreende a produção de sentidos realizada através da interação social do texto com seus coenunciadores e em Foucault (2013) um meio de entender como nossa sociedade atua disciplinarizando corpos, visando docilizá-los através de mecanismos como o heterossexismo diferencialista (BORRILLO, 2010). Com esse embasamento teórico analisou-se o Projeto de Decreto Legislativo n° 234 de 2011, conhecido popularmente como “projeto da cura gay”, de modo a identificar seu caráter intervencionista e legitimatório no meio social, por estar constituído dentro de um sistema de verdade, de controle, de disputas e de tensões de poder.
Discursos político-religiosos como armas de guerra: heteroterrorismo em ação contra sexualidades dissidentes
Authors: Fernandes Alexandre de Oliveira , De Souza Luciano Fernandes
Published: August 2020

Discursos político-religiosos estão implicados na construção de corpos e subjetividades generificadas. Organizando-se como uma arma de guerra, entrelaçam crenças e posicionamentos políticos, impõem padrões de normalidade, enfatizam ou desprezam determinadas condutas, reiterando discursos que seguem a heteronorma. Com vistas à construção de sujeitos e subjetividades normativizados – binários e dimórficos –, tratam o outro como abjeto, uma estratégia prático-discursiva que inferioriza aquele que se quer excluir do humano. Estigmatizando características corporais e psíquicas, legam aos sujeitos desviantes, ares de animalidade e de aberração. Defendemos neste artigo que tal interpelação discursiva emanada por discursos político-religiosos que atuam como armas de guerra, regula desejos, produz privações, punições e inscreve os dissidentes sexuais em uma alteridade cativa e repreendida, sob o signo de um “heteroterrorismo” (BENTO, 2017). Apoiamos nossa argumentação, especialmente em Roland Barthes (2004), Jacques Derrida (2001), Judith Butler (2017), Michel Foucault (2012) e Berenice Bento (2017).