bluuebunny/crossref_metadata_2025 huggingface dataset:
Sistem Informasi Reservasi Parkir Kendaraan Bermotor Berbasis Android
Authors: Mangunsong Ikhwanda , Yuda Fakhri Roza  , Nasution Salsabilla Sadedin
Published: March 2024

Seiring dengan perkembangan teknologi yang membantu pekerjaan manusia, sistem informasi menjadi salah satu perangkat yang dapat menyediakan layanan kepada user dalam berbagai hal. Sistem informasi dapat diklasifikasikan dalam berbagai bentuk, yakni berbasis website,berbasis desktop, maupun berbasis android. Sistem informasi berbasis android merupakan tipe yang kini tengah digandrungi oleh berbagai kalangan, karena penggunaannya yang mudah dan praktis, sehingga aplikasinya lebih direkomendasikan untuk digunakan pada saat ini. Sistem Informasi dapat dimanfaatkan dalam berbagai bidang, baik didalam maupun luar  lapangan, salah satu penerapannya diluar lapangan ialah penyewaan lahan parkir. Sehubungan dengan terlalu banyaknya pengemudi yang membutuhkan lokasi untuk memarkirkan kendaraannya, membuat para pengemudi kesulitan untuk menemukan tempat pemberhentian mereka, maka diperlukan suatu sistem untuk memesan lokasi parkir pada blok yang tersedia di lahan tersebut. Sistem Informasi Reservasi Parkir Kendaraan Bermotor Berbasis Android dibentuk dengan tujuan mempermudah petugas parkir dalam mereservasi lahan parkir bagi para klien (pengendara).
Peningkatan Kreatifitas Dan Pengetahuan Bina Lingkungan Pada Anak Berkebutuhan Khusus Melalui Media Aquaponik Di Slb C Muzdalifah
Authors: Anisah Siti
Published: March 2024

The contents of the abstraction, between 150-300 words, just one paragraph. Abstract includes the background or importance of community service topics, devotion goals, service partners, service methods and service results. If you use this template correctly, all numbering will be generated automatically. So you don't need to edit it manually. Of course, if you create a section of this paper that requires numbering after this template, the number will continue with the existing part number. Once again, you don't need to edit the part numbers, because if the contents of this template are deleted, the numbers will be sorted by themselves. If you want to try to delete the contents of this template, please back up first
Penerapan Learning Management System Untuk Pembelajaran Jarak Jauh Sekolah Menengah Atas
Authors: Irwan  , Hamdi  , Supiyandi
Published: March 2024

Sekitar 19,8 juta anak sekolah menengah di Indonesia saat ini belajar di bawah ancaman pandemi COVID-19. Untuk mencegah penularan COVID-19 di lingkungan sekolah, Menteri Pendidikan dan Kebudayaan telah mengeluarkan surat edaran tentang pembelajaran daring dan kerja dari rumah, yang mewajibkan seluruh sekolah menerapkan kebijakan “Belajar dari Rumah”. Kebijakan ini harus diterapkan mulai semester baru 2020-2021 dan perlu didukung oleh Learning ManagementSystem (LMS) beserta kompetensi seluruh pemangku kepentingan. Moodle dipilih karena gratis, dapat disesuaikan, dan dapat digunakan di server khusus untuk dikelola sepenuhnya oleh sekolah. Tahapan implementasi meliputi pengumpulan kebutuhan, instalasi dan konfigurasi, pengujian, pelatihan, dan evaluasi. Evaluasi tersebut mencatat beberapa kebutuhan untuk program selanjutnya, seperti menyederhanakan proses input untuk pertanyaan kuis, mengeksplorasi fitur yang lebih dalam, dan memberikan tutorial yang lebih informatif.
Pemanfaatan Google Form Membuat Analisis Soal Ujian Online Dalam Pembelajaran Jarak Jauh
Authors: Rizal Chairul , Supiyandi  , Iqbal Muhammad
Published: March 2024

Model ujian telah mengalami perubahan yang drastis, awalnya ujian diselenggarakan dalam bentuk Paper Base Test kini berubah menjadi Computer Base Test sesuai dengan perkembangan teknologi informasi. Tujuan pengabdian ini untuk memberikan kontribusi pengetahuan dalam pemanfaatan teknologi Google Form sebagai solusi dalam membuat analisis soal ujian online. Sebagai tahap awal, metode yang digunakan dalam pengabdian ini meliputi survei dan wawancara dengan para dewan guru, observasi serta pengumpulan data terkait pembelajaran dan studi pustaka sebagai dasar untuk mendapatkan referensi yang baik. Hasil dari pengabdian ini terciptanya analisis soal ujian dalam bentuk Form yang bisa didistribusikan ke siswa melalui link-link yang telah disiapkan.
SOSIALISASI PEMANFAATAN MEDIA SOSIAL UNTUK MENAMBAH INCOME BAGI IBU RUMAH TANGGA DUSUN IV DESA KELAMBIR LIMA KEBUN
Authors: Jodi Hendrawan  , Ika Devi Perwitasari  , Nadya Andhika Putri
Published: March 2024

Kegiatan sosialisasi pemanfaatan media sosial untuk meningkatkan pendapatan bagi ibu rumah tangga di Dusun IV, Desa Kelambir Lima Kebun adalah sebuah inisiatif yang bertujuan untuk memberdayakan dan mengembangkan potensi bisnis ibu rumah tangga melalui penggunaan media sosial. Penelitian ini menggunakan pendekatan partisipatif, di mana ibu rumah tangga aktif terlibat dalam proses sosialisasi. Melalui berbagai kegiatan, seperti pelatihan, lokakarya, dan diskusi kelompok, ibu rumah tangga diberikan pemahaman tentang potensi media sosial sebagai alat promosi dan penjualan produk atau jasa mereka. Hasil kegiatan sosialisasi menunjukkan adanya peningkatan kesadaran dan pengetahuan ibu rumah tangga mengenai manfaat media sosial dalam meningkatkan pendapatan keluarga. Mereka belajar bagaimana memanfaatkan berbagai platform media sosial untuk membangun merek, mencapai pasar yang lebih luas, dan menjalin hubungan dengan pelanggan potensial.
REFLEKSI AKHIR TAHUN DI SATUAN PENDIDIKAN (SMP PRIMBANA MEDAN)
Authors: Rusmin Saragih  , Gultom Imeldawaty , Sitompul Juliana Naftali , Harahap Ricky Ramadhan , Barany Fachri
Published: March 2024

Primbana Medan Middle School is one of the schools selected in the driving school program. Primbana Middle School received training from facilitators from the driving school program. Every month this school attends a meeting with the facilitator via zoom meeting/google meet to evaluate the results of the independent learning curriculum at the school. At the end of the 2022/2023 academic year, a year-end reflection was held to assess how the results of the Free Learning Curriculum program were at the school. The teaching method that we use is group discussion so that it makes it easier for fellow teachers to explain the results of reflection on learning according to the subject group taught and explained using cardboard that has been completed by the school, then fellow teachers explain in turn to see the effect in teaching in a better direction. Fellow teachers are better prepared for the demands of the independent learning curriculum and are more prepared to carry out activities related to the independent learning curriculum. Students are better prepared and trained to explore the basic knowledge they have and can develop it.
Optimalisasi Promosi Dalam Pengembangan Desa Wisata Berbasis Partisipasi Masyarakat Desa Sei Limbat
Authors: Rizal Chairul , Zen Muhammad , Hendry
Published: March 2024

Pengembangan desa wisata berbasis partisipasi masyarakat merupakan pendekatan yang bertujuan untuk meningkatkan potensi ekonomi dan sosial suatu daerah dengan melibatkan aktifitas partisipatif warga lokal. Penelitian ini bertujuan untuk mengoptimalkan strategi promosi dalam pengembangan Desa Wisata Sei Limbat, dengan fokus pada partisipasi masyarakat desa. Studi ini menggunakan metode penelitian kualitatif dan kuantitatif, dengan pengumpulan data melalui wawancara, survei, dan observasi lapangan. Hasil penelitian menunjukkan bahwa partisipasi aktif masyarakat desa memiliki peran penting dalam mengembangkan desa wisata. Namun, promosi desa wisata ini masih belum optimal dan perlu ditingkatkan. Dalam upaya meningkatkan promosi, penelitian ini mengidentifikasi berbagai faktor yang mempengaruhi partisipasi masyarakat, termasuk pengetahuan, minat, dan keterlibatan mereka dalam kegiatan promosi. Selain itu, studi ini juga mengidentifikasi hambatan dan kendala yang menghambat partisipasi masyarakat dalam promosi desa wisata. Hasil penelitian ini diharapkan dapat memberikan panduan dan rekomendasi kepada pemerintah daerah dan pemangku kepentingan terkait untuk meningkatkan promosi desa wisata Sei Limbat dengan memaksimalkan partisipasi masyarakat. Dengan demikian, pengembangan desa wisata berbasis partisipasi masyarakat dapat menjadi sarana yang efektif dalam mendorong pertumbuhan ekonomi dan kesejahteraan masyarakat desa Sei Limbat.
The Musical Expat: Privileged Migration and Baby Music Class in Lisbon
Authors: Snyder Andrew
Published: March 2024

This article focuses on the role of children’s music education in the settlement of Lisbon, Portugal, by “expats,” privileged migrants who build community not with others who share an ethnic identity but rather with other international residents of similar class status, a phenomenon accelerated by the advent of remote work. By calling attention to the multicultural and pop aesthetics used in baby music classes at the Music Room, a private music school conducted primarily in English and marketed to expats, I show how expat children are sonically enculturated in cosmopolitan spheres and how expat cultural infrastructure plays a crucial role in promoting expat migration.
Antropogēno un dabisko apdraudējumu mazināšana ar viedās apdrošināšanas līgumiem
Authors:   , Jonathan Pagano Andrea
Published: March 2024

Promocijas darba mērķis ir izpētīt apdrošināšanas daudzpusīgo lomu indivīda, kopienas un sabiedrības aizsardzībā pret sociālo un dabas katastrofu radīto finansiālo slogu. Pētījums sniedz visaptverošu izpratni par dinamiskajiem mehānismiem, kurus izmantojot, apdrošināšana darbojas kā riska pārnešanas un mazināšanas instruments, pārbaudot arī apdrošināšanas potenciālu ietekmēt sagatavošanos katastrofām, izturību un pilsētu un sabiedrības pielāgošanos. Pētījumā apskatīta arī katastrofu apdrošināšanas loma, problēmas un iespējas, ko tā sniedz arvien neparedzamākā klimata apstākļos.
Współpraca nauki z biznesem w obszarze energetyki rozproszonej
Authors: Skomudek Waldemar
Published: March 2024

Obecnie znajdujemy się w okresie zmian gospodarczych, które traktujemy jako zaawansowany i dynamicznie przebiegający proces transformacji energetycznej. Z założenia proces ten zmierza do wytworzenia bardziej konkurencyjnego, bezpiecznego i zrównoważonego systemu elektroenergetycznego oraz osiągnięcia długoterminowego celu ograniczenia emisji gazów cieplarnianych. Treść artykułu nawiązuje w szczególności do energetyki rozproszonej, której intensywny rozwój wyzwala ogromną potrzebę zacieśnienia współpracy nauki i biznesu. Zrównoważona współpraca tych obszarów powinna prowadzić do rozwoju techniki i innowacyjnych technologii, a także do wytworzenia nowej kadry oraz własności intelektualnej.
Aging and life control of cross-linked polyethylene as cable insulation material
Authors: Wang Jiang-Qiong , Li Wei-Kang , Zhang Wen-Ye , Wan Bao-Quan , Zha Jun-Wei ,   Beijing Advanced Innovation Center for Materials Genome Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China,   Beijing Institute of Smart Energy, Beijing 102211, China,   Shunde Innovation School, University of Science and Technology Beijing, Foshan 528300, China
Published: March 2024

Cross-linked polyethylene (XLPE) has been widely used in the field of power cables due to its excellent mechanical properties and insulating properties. However, during the manufacturing of high voltage cables, XLPE will inevitably be affected by electrical aging, thermal aging and electro-thermal combined aging, which makes the resistance and life of the material decline. Therefore, it is necessary to enhance the aging resistance of XLPE without affecting its mechanical properties and insulating properties, so as to extend its service life. In this work, the structural characteristics and cross-linking mechanism of XLPE are introduced, the aging process and influencing mechanism are systematically analyzed, and the life decay problems of XLPE due to aging are explored by using methods such as the temperature Arrhenius equation and the inverse power law of voltage. The improvement strategies such as grafting, blending, and nanoparticle modification can be used to enhance the thermal stability, antioxidant properties, and thermal aging resistance of XLPE, thereby extending its service life. Finally, the strategies of adjusting and controlling the service life of XLPE cable insulation materials in the future are discussed, which provide theoretical guidance for further improving long-term stable operation of XLPE cable insulation materials.
The BTB-ZF gene Bm-mamo regulates pigmentation in silkworm caterpillars
Authors: Wu Songyuan , Tong Xiaoling , Peng Chenxing , Luo Jiangwen , Zhang Chenghao , Lu Kunpeng , Li Chunlin , Ding Xin , Duan Xiaohui , Lu Yaru , Hu Hai , Tan Duan , Dai Fangyin
Published: March 2024

The color pattern of insects is one of the most diverse adaptive evolutionary phenotypes. However, the molecular regulation of this color pattern is not fully understood. In this study, we found that the transcription factor Bm-mamo is responsible for
black dilute
(
bd
) allele mutations in the silkworm. Bm-mamo belongs to the BTB zinc finger family and is orthologous to mamo in
Drosophila melanogaster
. This gene has a conserved function in gamete production in
Drosophila
and silkworms and has evolved a pleiotropic function in the regulation of color patterns in caterpillars. Using RNAi and clustered regularly interspaced short palindromic repeats (CRISPR) technology, we showed that Bm-mamo is a repressor or has dark melanin patterns in the larval epidermis. Using in vitro binding assays and gene expression profiling in wild-type and mutant larvae, we also showed that
Bm-mamo
likely regulates the expression of related pigment synthesis and cuticular protein genes in a coordinated manner to mediate its role in color pattern formation. This mechanism is consistent with the dual role of this transcription factor in regulating both the structure and shape of the cuticle and the pigments that are embedded within it. This study provides new insight into the regulation of color patterns as well as into the construction of more complex epidermis features in some insects.
Machine learning and biological validation identify sphingolipids as key mediators of paclitaxel-induced neuropathy in cancer patients
Authors: Lötsch Jörn , Gasimli Khayal , Malkusch Sebastian , Hahnefeld Lisa , Angioni Carlo , Schreiber Yannick , Trautmann Sandra , Wedel Saskia , Thomas Dominique , Bouzas Nerea Ferreiros , Brandts Christian , Schnappauf Benjamin , Solbach Christine , Geisslinger Gerd , Sisignano Marco
Published: March 2024

Chemotherapy-induced peripheral neuropathy (CIPN) is a serious therapy-limiting side effect of commonly used anticancer drugs. Previous studies suggest that lipids may play a role in CIPN. Therefore, the present study aimed to identify the particular types of lipids that are regulated as a consequence of paclitaxel administration and may be associated with the occurrence of post-therapeutic neuropathy.High resolution mass spectrometry lipidomics was applied to quantify d = 255 different lipid mediators in the blood of n = 31 patients drawn before and after paclitaxel therapy for breast cancer treatment. A variety of supervised statistical and machine-learning methods was applied to identify lipids that were regulated during paclitaxel therapy or differed among patients with and without post-therapeutic neuropathy.Twenty-seven lipids were identified that carried relevant information to train machine learning algorithms to identify, in new cases, whether a blood sample was drawn before or after paclitaxel therapy with a median balanced accuracy of up to 90%. One of the top hits, sphinganine-1-phosphate (SA1P), was found to induce calcium transients in sensory neurons via the transient receptor potential vanilloid 1 (TRPV1) channel and sphingosine-1-phosphate receptors.SA1P also showed different blood concentrations between patients with and without neuropathy.Present findings suggest an important role for sphinganine-1-phosphate in paclitaxel-induced biological changes associated with neuropathic side effects. The identified SA1P, through its receptors, provides a potential drug target for co-therapy with paclitaxel to reduce one of its major and therapy-limiting side effects.
Ocular dominance-dependent binocular combination of monocular neuronal responses in macaque V1
Authors: Zhang Sheng-Hui , Zhao Xing-Nan , Tang Shi-Ming , Yu Cong
Published: March 2024

Primates rely on two eyes to see depth, while keeping a stable vision when one eye is closed. Although psychophysical and modeling studies have investigated how monocular signals are combined to form binocular vision, the corresponding neuronal mechanisms, especially in V1 where most neurons become binocular but with different eye preferences, are not well understood. Here we used two-photon calcium imaging to compare monocular and binocular responses of thousands of V1 superficial-layer neurons in three awake macaques. Under monocular stimulation, neurons preferring the stimulated eye responded substantially stronger than those preferring both eyes. However, under binocular stimulation, the responses of neurons preferring either eye were suppressed, and those preferring both eyes were enhanced, so that neuronal responses became similar regardless of eye preferences. A neuronally realistic model of binocular combination, which includes ocular dominance-dependent divisive interocular inhibition, and binocular summation, is proposed to account for these observations.
Export of discarded splicing intermediates requires mRNA export factors and the nuclear basket
Authors: Zeng Yi , Staley Jonathan P.
Published: March 2024

To promote fidelity in nuclear pre-mRNA splicing, the spliceosome rejects and discards suboptimal splicing substrates after they have engaged the spliceosome. Although nuclear quality control mechanisms have been proposed to retain immature mRNPs, evidence indicates that discarded splicing substrates, including lariat intermediates, do export to the cytoplasm, as indicated by their translation and degradation by cytoplasmic nucleases. However, the mechanism for exporting these species has remained unknown. By single molecule (sm) RNA FISH in budding yeast, we have directly observed the nuclear export of lariat intermediates. Further, by crosslinking, export reporter assays, and smRNA FISH, we have demonstrated that the export of lariat intermediates requires the general mRNA export receptor Mex67p and three of its mRNA export adapter proteins, Nab2p, Yra1p, and Nlp3, establishing that mRNAs and lariat intermediates share the same export machinery. Unexpectedly, the export of lariat intermediates, but not mRNA, requires an interaction between Nab2p and Mlp1p, a nuclear basket component implicated in retaining immature mRNPs, including unspliced pre-mRNA, in the nucleus of budding yeast. Finally, the export of lariat intermediates, like mRNA, relies on the E3 ubiquitin ligase Tom1p and its target sites in Yra1p. Overall, our data indicate that the nuclear basket can promote, rather than antagonize, the export of an immature mRNP. Further, our data imply that the export of discarded lariat intermediates requires both Mlp1p-dependent docking onto the nuclear basket and subsequent Tom1p-mediated undocking, a mechanism our data suggests functions in the export of mRNA also but in a manner obscured by redundant pathways.
Rab10 regulates neuropeptide release by maintaining Ca2+ homeostasis and protein synthesis
Authors: Dong Jian , Chen Miao , van Weering Jan RT , Li Ka Wan , Smit August B , Toonen Ruud F , Verhage Matthijs
Published: March 2024

Dense core vesicles (DCVs) transport and release various neuropeptides and neurotrophins that control diverse brain functions, but the DCV secretory pathway remains poorly understood. Here, we tested a prediction emerging from invertebrate studies about the crucial role of the intracellular trafficking GTPase Rab10, by assessing DCV exocytosis at single-cell resolution upon acute Rab10 depletion in mature mouse hippocampal neurons, to circumvent potential confounding effects of Rab10’s established role in neurite outgrowth. We observed a significant inhibition of DCV exocytosis in Rab10-depleted neurons, whereas synaptic vesicle exocytosis was unaffected. However, rather than a direct involvement in DCV trafficking, this effect was attributed to two ER-dependent processes, ER-regulated intracellular Ca
2+
dynamics and protein synthesis. Gene ontology analysis of differentially expressed proteins upon Rab10 depletion identified substantial alterations in synaptic and ER/ribosomal proteins, including the Ca
2+
-pump SERCA2. In addition, ER morphology and dynamics were altered, ER Ca
2+
levels were depleted and Ca
2+
homeostasis was impaired in Rab10-depleted neurons. However, Ca
2+
entry using a Ca
2+
ionophore still triggered less DCV exocytosis. Instead, leucine supplementation, which enhances protein synthesis, largely rescued DCV exocytosis deficiency. We conclude that Rab10 is required for neuropeptide release by maintaining Ca
2+
dynamics and regulating protein synthesis. Furthermore, DCV exocytosis appeared more dependent on (acute) protein synthesis than synaptic vesicle exocytosis.
Rab10 regulates neuropeptide release by maintaining Ca2+ homeostasis and protein synthesis
Authors: Dong Jian , Chen Miao , van Weering Jan R. T. , Li Ka Wan , Smit August B , Toonen Ruud F. , Verhage Matthijs
Published: March 2024

Dense core vesicles (DCVs) transport and release various neuropeptides and neurotrophins that control diverse brain functions, but the DCV secretory pathway remains poorly understood. Here, we tested a prediction emerging from invertebrate studies about the crucial role of the intracellular trafficking GTPase Rab10, by assessing DCV exocytosis at single- cell resolution upon acute Rab10 depletion in mature mouse hippocampal neurons, to circumvent potential confounding effects of Rab10’s established role in neurite outgrowth.We observed a significant inhibition of DCV exocytosis in Rab10-depleted neurons, whereas synaptic vesicle exocytosis was unaffected. However, rather than a direct involvement in DCV trafficking, this effect was attributed to two ER-dependent processes, ER-regulated intracellular Ca
2+
dynamics and protein synthesis. Gene ontology analysis of differentially expressed proteins upon Rab10 depletion identified substantial alterations in synaptic and ER/ribosomal proteins, including the Ca
2+
-pump SERCA2. In addition, ER morphology and dynamics were altered, ER Ca
2+
levels were depleted and Ca
2+
homeostasis was impaired in Rab10-depleted neurons. However, Ca
2+
entry using a Ca
2+
ionophore still triggered less DCV exocytosis. Instead, leucine supplementation, which enhances protein synthesis, largely rescued DCV exocytosis deficiency. We conclude that Rab10 is required for neuropeptide release by maintaining Ca
2+
dynamics and regulating protein synthesis. Furthermore, DCV exocytosis appeared more dependent on (acute) protein synthesis than synaptic vesicle exocytosis.
Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution
Authors: Merino-Salomón Adrián , Schneider Jonathan , Babl Leon , Krohn Jan-Hagen , Sobrinos-Sanguino Marta , Schäfer Tillman , Luque-Ortega Juan R. , Alfonso Carlos , Jiménez Mercedes , Jasnin Marion , Schwille Petra , Rivas Germán
Published: March 2024

In most bacteria, division depends on a cytoskeletal structure, the Z ring, which serves as a scaffold for recruiting additional proteins, with which it forms the machinery responsible for division, the divisome. The detailed architecture of the ring, in particular the mechanisms of assembly, stabilization, and disassembly, are still largely unknown. Here, we highlight the role of FtsZ-associated proteins (Zaps) in stabilizing the Z ring by crosslinking the filaments. Among Zap proteins, ZapD binds the C-terminal domain of FtsZ, which serves as a hub for its regulation. We demonstrate that ZapD crosslinks FtsZ filaments in solution into toroidal structures formed by an arrangement of short, curved filaments. Using cryo-electron tomography combined with biochemical analysis, we reveal the three-dimensional organization of FtsZ within the toroids, shedding light on the crosslinking mechanism by ZapD. In spite of the compositional simplicity of our reconstituted system, the structural organization of the FtsZ polymers by ZapD appears to be compatible with the current model of the Z ring in the bacterial cell.
Loss of ZNRF3/RNF43 Unleashes EGFR in Cancer
Authors: Yue Fei , Ku Amy T , Stevens Payton D , Michalski Megan N , Jiang Weiyu , Tu Jianghua , Shi Zhongcheng , Dou Yongchao , Wang Yi , Feng Xin-Hua , Hostetter Galen , Wu Xiangwei , Huang Shixia , Shroyer Noah F , Zhang Bing , Williams Bart O , Liu Qingyun , Lin Xia , Li Yi
Published: March 2024

ZNRF3 and RNF43 are closely related transmembrane E3 ubiquitin ligases with significant roles in development and cancer. Conventionally, their biological functions have been associated with regulating WNT signaling receptor ubiquitination and degradation. However, our proteogenomic studies have revealed EGFR as the protein most negatively correlated with
ZNRF3/RNF43
mRNA levels in multiple human cancers. Through biochemical investigations, we demonstrate that ZNRF3/RNF43 interact with EGFR via their extracellular domains, leading to EGFR ubiquitination and subsequent degradation facilitated by the E3 ligase RING domain. Overexpression of
ZNRF3
reduces EGFR levels and suppresses cancer cell growth
in vitro
and
in vivo
, whereas knockout of
ZNRF3
/
RNF43
stimulates cell growth and tumorigenesis through upregulated EGFR signaling. Together, these data highlight ZNRF3 and RNF43 as novel E3 ubiquitin ligases of EGFR and establish the inactivation of ZNRF3/RNF43 as a driver of increased EGFR signaling, ultimately promoting cancer progression. This discovery establishes a connection between two fundamental signaling pathways, EGFR and WNT, at the level of cytoplasmic membrane receptors, uncovering a novel mechanism underlying the frequent co-activation of EGFR and WNT signaling in development and cancer.
Overview of chicken embryo genes related to sex differentiation
Authors: Luo Xiaolu , Guo Jiancheng , Zhang Jiahang , Ma Zheng , Li Hua
Published: March 2024

Sex determination in chickens at an early embryonic stage has been a longstanding challenge in poultry production due to the unique ZZ:ZW sex chromosome system and various influencing factors. This review has summarized the genes related to the sex differentiation of chicken early embryos (mainly Dmrt1, Sox9, Amh, Cyp19a1, Foxl2, Tle4z1, Jun, Hintw, Ube2i, Spin1z, Hmgcs1, Foxd1, Tox3, Ddx4, cHemgn and Serpinb11 in this article), and has found that these contributions enhance our understanding of the genetic basis of sex determination in chickens, while identifying potential gene targets for future research. This knowledge may inform and guide the development of sex screening technologies for hatching eggs and support advancements in gene-editing approaches for chicken embryos. Moreover, these insights offer hope for enhancing animal welfare and promoting conservation efforts in poultry production.
Endosymbiont and gut bacterial communities of the brown-banded cockroach, Supella longipalpa
Authors: Guse Kylene , Pietri Jose E.
Published: March 2024

The brown-banded cockroach (Supella longipalpa) is a widespread nuisance and public health pest. Like the German cockroach (Blattella germanica), this species is adapted to the indoor biome and completes the entirety of its life cycle in human-built structures. Recently, understanding the contributions of commensal and symbiotic microbes to the biology of cockroach pests, as well as the applications of targeting these microbes for pest control, have garnered significant scientific interest. However, relative to B. germanica, the biology of S. longipalpa, including its microbial associations, is understudied. Therefore, the goal of the present study was to quantitatively examine and characterize both the endosymbiont and gut bacterial communities of S. longipalpa for the first time. To do so, bacterial 16S rRNA gene amplicon sequencing was conducted on DNA extracts from whole adult females and males, early instar nymphs, and late instar nymphs. The results demonstrate that the gut microbiome is dominated by two genera of bacteria known to have beneficial probiotic effects in other organisms, namely Lactobacillus and Akkermansia. Furthermore, our data show a significant effect of nymphal development on diversity and variation in the gut microbiome. Lastly, we reveal significant negative correlations between the two intracellular endosymbionts, Blattabacterium and Wolbachia, as well as between Blattabacterium and the gut microbiome, suggesting that Blattabacterium endosymbionts could directly or indirectly influence the composition of other bacterial populations. These findings have implications for understanding the adaptation of S. longipalpa to the indoor biome, its divergence from other indoor cockroach pest species such as B. germanica, the development of novel control approaches that target the microbiome, and fundamental insect-microbe interactions more broadly.
TBX5 genetic variants and SCD-CAD susceptibility: insights from Chinese Han cohorts
Authors: Rui Yukun , Zhou Ju , Zhen Xiaoyuan , Zhang Jianhua , Liu Shiquan , Gao Yuzhen
Published: March 2024

Background

The prevention and prediction of sudden cardiac death (SCD) present persistent challenges, prompting exploration into common genetic variations for potential insights. T-box 5 (TBX5), a critical cardiac transcription factor, plays a pivotal role in cardiovascular development and function. This study systematically examined variants within the 500-bp region downstream of the TBX5 gene, focusing on their potential impact on susceptibility to SCD associated with coronary artery disease (SCD-CAD) in four different Chinese Han populations.

Methods

In a comprehensive case-control analysis, we explored the association between rs11278315 and SCD-CAD susceptibility using a cohort of 553 controls and 201 SCD-CAD cases. Dual luciferase reporter assays and genotype-phenotype correlation studies using human cardiac tissue samples as well as integrated in silicon analysis were applied to explore the underlining mechanism.

Result

Binary logistic regression results underscored a significantly reduced risk of SCD-CAD in individuals harboring the deletion allele (odds ratio = 0.70, 95% CI [0.55–0.88], p = 0.0019). Consistent with the lower transcriptional activity of the deletion allele observed in dual luciferase reporter assays, genotype-phenotype correlation studies on human cardiac tissue samples affirmed lower expression levels associated with the deletion allele at both mRNA and protein levels. Furthermore, our investigation revealed intriguing insights into the role of rs11278315 in TBX5 alternative splicing, which may contribute to alterations in its ultimate functional effects, as suggested by sQTL analysis. Gene ontology analysis and functional annotation further underscored the potential involvement of TBX5 in alternative splicing and cardiac-related transcriptional regulation.

Conclusions

In summary, our current dataset points to a plausible correlation between rs11278315 and susceptibility to SCD-CAD, emphasizing the potential of rs11278315 as a genetic risk marker for aiding in molecular diagnosis and risk stratification of SCD-CAD.
Framework for self-organizing resilient ad-hoc networks using hierarchical routing
Authors:   , Ladrix Aguilera Tomás Andrés
Published: March 2024

La resiliencia ha sido objeto de estudio en varios campos de estudio. En lo que respecta a redes e infraestructura de telecomunicaciones, no hay consenso sobre cómo definir, medir e implementar sistemas resilientes. Generalmente se analiza como una propiedad que se encuentra a nivel de Capa de Red, proponiendo soluciones y protocolos resilientes asumiendo que las capas inferiores son capaces de proporcionar una comunicación confiable. Se considera a menudo que los nodos de una red tienen un alcance fijo para comunicarse confiablemente con sus vecinos, siendo inalcanzables fuera de éste. Sin embargo, en la práctica, fenómenos como el desvanecimiento de canal contradicen esta idea. En este trabajo, se elaboran conceptos y se propone un marco para entender y evaluar la resiliencia en el contexto de redes inalámbricas ad-hoc. Esta propuesta involucra elementos tanto en la Capa de Red (NET) como en la Capa de Enlace (DLL), con la consideración clave de tener en cuenta la existencia de canales no confiables entre nodos. El objetivo es otorgar a la red la capacidad de auto-organización y adaptación, como una forma de proporcionar comportamiento resiliente a través de un sistema organizacional que sea capaz de adaptarse a las disrupciones que ocurren en la red. Se implementan tres componentes que consisten en un Protocolo de Enrutamiento Jerárquico (HRP), encargado de mantener y estructurar el sistema organizacional, un Protocolo de Recuperación de Rutas (RRP) que actúa como un mecanismo de recuperación en caso de fallas de nodos, y un Estimador de Confiabilidad de Vecinos (NRE) que sirve para informar sobre la confiabilidad de los vecinos de cada nodo. En conjunto permiten la operación descentralizada, autónoma y auto-configurable de los nodos. Además, permiten una operación cohesionada de la red, constituyendo la contribución de este trabajo.
Optimization-based simulation framework for large-scale hydrothermal power systems through interconnected models
Authors:   , Riquelme Gajardo Tomás Eduardo
Published: March 2024

El marco propuesto en esta tesis evalúa modelos operacionales que gestionan agua en la generación eléctrica. Este trabajo está motivado por dos consideraciones principales. En primer lugar, la escasez global hídrica requiere gestión inteligente del agua para conservarla en el futuro. En segundo lugar, la necesaria descarbonización de la industria eléctrica posee desafíos por la integración de nuevas tecnologías, nuevas dinámicas operacionales y la mantención de resiliencia de la red. El marco comprende tres modelos interconectados: un modelo de largo plazo, generando condiciones de término para el modelo de mediano plazo; un modelo de mediano plazo, determinando el valor del agua al considerar el impacto de su uso en los costos actuales y futuros, y sirviendo como dato de entrada esencial para el modelo de corto plazo, donde el valor del agua es utilizado como factor de costo por utilizar agua de embalses; y un modelo de corto plazo, representando en mayor detalle el desempeño de la red en comparación con los otros modelos. En nuestra simulación, adoptamos un enfoque de horizonte en ejecución, ejecutando los modelos de manera semanal y utilizando los resultados del modelo de corto plazo de una semana como condiciones iniciales para los modelos de la siguiente semana. Este trabajo considera cuatro experimentos para analizar cómo el cambio en detalles del modelo de mediano plazo afecta en el desempeño. Los hallazgos destacan al marco propuesto como una valiosa herramienta para evaluar el impacto de cambiar características de modelos en el tiempo. Nuestros experimentas muestran que refinar el detalle de corto plazo en el modelo de mediano plazo eleva el valor del agua, resultando en incrementos del nivel de los embalses y los costos operacionales al hacerse más atractivo guardar agua, requiriendo otras tecnologías para compensar los déficits de generación.
Actos escriturales a ras de suelo: Infrabildungsroman e infrapolítica en cinco guiones óperaprimistas chilenos
Authors:   , Leiva Barahona Carlos
Published: March 2024

Esta investigación revisa cinco guiones óperaprimistas chilenos, “Volantín cortao” de Diego Ayala & Aníbal Jofré, “Mala Junta” de Claudia Huaiquimilla & Pablo Greene, “El primero de la familia” de Carlos Leiva Barahona, “Niñas Araña” de Guillermo Helo y “Piola” de Luis Pérez, todos escritos entre 2014 y 2018, a partir de sus actos escriturales con el propósito de entenderlos como escrituras infrapolíticas del presente: a ras de suelo, desde la vivencia propia de los guionistas, la investigación comprometida, la interdisciplina y el contexto de crisis institucional imperante en el Chile pre-estallido. Estos guionistas ––que también son los directores de las películas–– decididamente buscan reflexionar sobre nuestro país y sus grandes problemáticas, pero sin hacer explícitos llamados a la activación política. Más bien buscan proponer una nueva perspectiva, una más infra, desde un punto de vista narrativo centrado en la emoción que suscitan personajes jóvenes que subyacen en los márgenes del neoliberalismo. En sus escrituras, cuyo modus operandi es distinto a las lógicas tradicionales de la industria cinematográfica, se destaca la prominencia de un nuevo tropo literario que denomino infrabildungsroman (bildungsroman modo infra), el cual me sirve para describir al joven infra presente en las ficciones: adolescentes marginales que sobreviven el mundo desde la fractura con sus estructuras parentales (Estado, familia, colegio) y una horizontalidad a la hora de plantear sus relaciones con los pares.
Prevalence of Exercise-induced Desaturation in Survivors of Severe COVID-19 Pneumonia and the Predictive Value of Lung Ultrasound: A Cross-sectional Study
Authors: Praveen GS , Suraj KP , Mohamed N Safreena
Published: March 2024

Introduction: Survivors of severe Coronavirus Disease2019 (COVID-19) pneumonia may experience ExerciseInduced Desaturation (EID), which can remain undiagnosed at discharge, as most exhibit normal resting Arterial Blood Gases (ABGs). This undiagnosed condition may lead to unexpected hospital readmissions, causing additional burden to the health system. However, the prevalence of EID among this population, especially in developing countries, is not well documented. Aim: To estimate the prevalence of EID among survivors of severe COVID-19 pneumonia and to explore the predictive role of Lung Ultrasound Scan (LUS) in detecting EID. Materials and Methods: This hospital-based, observational, cross-sectional study was conducted at the Institute of Chest Diseases in Kozhikode, Kerala, India from October 2021 to September 2022. It involved 153 COVID-19 patients aged 18 years and above who had recovered from severe pneumonia, were otherwise fit for hospital discharge, and had normal resting ABGs. The study examined the prevalence of EID using the 6-Minute Walk Test (6MWT). Additionally, it investigated the predictive ability of a LUS for EID. A LUS was performed on all patients and scoring was conducted according to the established protocol. Results: The mean age of the study subjects was 53.50±14.13 years, with 119 (77.8%) being male. During the 6MWT, 96 patients (62.7%) showed significant desaturation. A total of 83 (95.4%) subjects in the desaturated group required Non-Invasive Ventilation (NIV) compared to 4 (4.6%) in the non-desaturated group (p<0.001). All 24 (100%) subjects in the desaturated group required Invasive Mechanical Ventilation (IMV), while none in the non-desaturated group did. The mean Length Of Stay (LOS) in the hospital was 35.50±13.35 days for the desaturated group, compared to 23.32±16.25 days for the non-desaturated group (p<0.001). The LUS score was significantly higher in the desaturated group (16.61±5.92) than in the non-desaturated group (5.54±4.62). The Receiver Operating Characteristic (ROC) curve for the LUS score indicated a cut-off value of 8.5 for identifying significant EID. Conclusion: Survivors of COVID-19 who recovered from Acute Respiratory Failure (ARF) and have normal resting ABGs may still have significant EID. A protocol-based LUS score can potentially identify individuals at risk for EID.
Allergic Reaction to Aminophylline in a Case of Lower Respiratory Tract Infection with Measles: A Rare Case Report
Authors: Handargule Anuja Satish , Taksande Amar , Meshram Revat , Uke Poonam
Published: March 2024

Measles is an acute respiratory infectious disease caused by the measles virus. It leads to respiratory involvement with manifestations such as pneumonia, laryngobronchitis, pneumonitis, etc. It can also cause secondary bacterial and fungal infections. Aminophylline is a methylxanthine bronchodilator composed of theophylline and ethylenediamine. Airway blockage is reversed by bronchial smooth muscle relaxation, increased myosin light chain kinase activity, and decreased intracellular calcium concentration. It relaxes the smooth muscle of the bronchial airways and pulmonary blood vessels, reducing airway responsiveness to histamine, methacholine, adenosine, and allergens. Allergic skin reactions secondary to aminophylline administration have been rarely seen. Aminophylline can be given orally as well as intravenously. A seven-month-old male infant presented with a lower respiratory tract infection and a maculopapular rash involving the face and trunk. A history of contact with measles was present. Intravenous aminophylline was administered for persistent wheezing. The infant developed a generalised erythematous papular rash with a more widespread distribution than before within one hour of administration. The child was treated with antihistamines for the same. After a detailed physical examination, looking at the pattern of the rash and excluding other causes like environmental factors and drug history, the diagnosis of an allergic reaction to aminophylline was made. Early identification of allergic reactions is paramount, as prompt cessation of the offending agent and initiation of appropriate medical interventions can significantly mitigate the severity of the reaction and prevent potential life-threatening complications.
Meropenem-induced Haematological Abnormalities in the Elderly: A Series of Three Cases
Authors: Rao Sharadashri , Vaidya Kuladeepa Ananda
Published: March 2024

Meropenem is a broad-spectrum antibacterial agent given empirically for diseases caused by single or multiple susceptible bacteria in both adults and children with a wide range of serious infections. Several studies have reported an association between meropenem and haematological abnormalities such as leukopenia, thrombocytopenia, neutropenia, and haemolytic anaemia. In this case series, the authors present three cases - one with meropenem-induced leukopenia and the other two with meropeneminduced thrombocytopenia. In the first case, leukopenia was observed on the third day of administration. The leukocyte count dropped to 3,500 cells/mcL but recovered upon discharge. In the other two cases, the platelet count dropped below 1.3 lac/cu.mm on the fourth day of administration but started to recover once the antibiotic was discontinued. This underscores the importance of being vigilant and conducting regular laboratory blood counts when administering antibiotics like meropenem to prevent lifethreatening complications.
Phenology and Climate Indices of Maize Hybrids under North Western Himalayan Region of Temperate Kashmir
Authors: Mir Suhail A , Ahmad Latief , Qayoom Kahkashan , Rasool Faisul Ur , Mir Aamir Hassan , Bhat Raies A , Bhat Owais A , Ahmad Roheela , Pandit Bilal Ahmad
Published: March 2024

In the Kharif season of 2021, a field experiment was conducted at the crop research farm of the Division of Agronomy, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir. The primary objective of the study was to investigate the phenology of maize hybrids in relation to agrometeorological indices. The experimental design included two factors: Factor A, representing different irrigation regimes at various growth stages (I1 to I6), and Factor B, consisting of two maize hybrid varieties (Shalimar Hybrid-2 and Shalimar Hybrid-3). The irrigation treatments ranged from early vegetation stage to rainfed conditions. The experiment followed a split plot design with three replications. Results indicated that the treatment I5 (Irrigation at IW/CPE ratio of 0.8) and Shalimar Hybrid-3 exhibited the highest values for key agrometeorological indices, including GDD, HTU, PTU, and HYTU. These findings provide valuable insights into optimizing irrigation practices and selecting suitable maize hybrids for enhanced phenological performance.
Studies on the Sex Ratio of Different Mango Varieties under the Northern Dry Zone of Karnataka
Authors: Sushmitha B. H.  , Bhyravi B. M.  , Allolli T. B.  , Kulapati H.  , Awati Mallikarjun , Kurabetta Krishna , Jain Shubham
Published: March 2024

An investigation was carried out to evaluate different cultivars of mango for their performance, growth, and flowering under the northern dry zone of Karnataka. Tree height, canopy volume and trunk girth were maximum in Baneshan, while Kadar recorded maximum tree spread. Baneshan were the first to come into full bloom, and Dashehari were recorded maximum panicles per plant. The maximum number of fruits per panicle at harvest was found in Kesar. The maximum fruit set per cent was observed in Totapuri
The Revolutionary Impact of Regenerative Agriculture on Ecosystem Restoration and Land Vitality: A Review
Authors: Vamshi Maragani , Jagadeesan R. , Lamani Hanumanta D , Rout Sandeep , VijayKumar R , Jagadesh M  , Sachan Kushal
Published: March 2024

The revolutionary impact of regenerative agriculture on ecosystem restoration and land vitality in India delineates the nuances of its principles, practices, and the symbiotic relationship with community engagement and policy frameworks. Regenerative agriculture, a holistic approach prioritizing soil health, biodiversity, water management, and carbon sequestration, emerges as a critical solution to the challenges of soil degradation, biodiversity loss, and climate change faced by Indian agriculture. Through a synthesis of comparative studies and case analyses, this review highlights the tangible benefits of regenerative practices, such as enhanced soil structure, fertility, and microbial health, alongside improved water infiltration and conservation, underscoring the pivotal role of biodiversity in bolstering farm resilience and ecological balance. The narrative further delves into the socio-economic dimensions, examining the economic viability, knowledge dissemination, and the vital role of measurement and verification in scaling regenerative practices. Community and societal engagement, pivotal for fostering consumer demand for regeneratively produced products and collective restoration efforts, is identified as a cornerstone for the transition towards sustainable agriculture. Moreover, the review identifies the need for long-term impact studies to monitor ecosystem changes and assess global scalability. By integrating scientific research with policy analysis, the review advocates for innovations in regenerative techniques, aligned with precision agriculture, and underscores the necessity of supportive policy and economic incentives to catalyze the transition towards regenerative agriculture in India, thereby contributing to global food security and environmental sustainability.