bluuebunny/crossref_metadata_2025 huggingface dataset:
Cold tolerance of the aphid predator Episyrphus balteatus (DeGeer) (Diptera, Syrphidae)
Authors: HART A. J. , BALE J. S.
Published: March 2008

Abstract. Larvae of the hoverfiy Episyrphus balteatus (DeGeer) are important predators of aphids in the U.K. A large proportion of the U.K. population migrates south to warmer climes at the end of summer, but a small number are thought to overwinter in the U.K., with the mated female being the overwintering morph. The cold tolerance of adult flies was investigated to assess the overwintering potential of E. balteatus in the U.K. The high supercooling point (SCP) of ‐8.3 ± 0.7°C, and lethal temperature (LTemp30) of ‐9.1°C for acclimated females suggest that E. balteatus has limited cold hardiness. This was confirmed by experiments where, despite a strong acclimation response in both males and females, there was no long‐term survival at 5, 0 or ‐ 5°C. At 5°C, 90% of females had died after 10 days. The weak cold hardiness of adult E. balteatus was corroborated by field experiments which demonstrated a 100% mortality after 10 weeks' exposure to U.K. winter conditions. The ecological significance of this limited cold hardiness is discussed in relation to the overwintering abilities of E. balteatus in the U.K.
Oxygen uptake in coprophilous beetles (Aphodius, Geotrupes, Sphaeridium) at low oxygen and high carbon dioxide concentrations
Authors: HOLTER PETER , SPANGENBERG ANNETTE
Published: March 2008

Abstract.The rate of O2consumption was measured in five coprophilous beetle species (common in Denmark) at O2concentrations from 1–21%. With the exception of the mainly soil‐livingGeotrupes spiniger(Marsham) (Geotrupidae), these beetles are probably exposed to severe hypoxia in fresh cattle pats.Aphodius fossor(Linnaeus),A. contaminatus(Herbst) (Aphodiidae) andSphaeridium lunatumFabricius (Hydrophilidae) maintained normal movements and a normal rate of 02uptake (for at least 30 min) at only 1% O2. There is no evidence, therefore, that the beetles switch to anaerobic metabolism under these conditions. This ability to regulate respiration, and hence to extract 02at very low concentrations, is exceptional even among terrestrial arthropods living in soil or other potentially hypoxic substrates. InA. rufipes(Linnaeus), respiration declined at ambient concentrations below 2% O2, and inG. spinigerthe ability to regulate respiration seemed to fail at even higher concentrations. In four of the species (G.spinigerwas not tested), about 11% CO2(the level in a dung pat at 2% O2) did not affect the O2uptake at 2% O2.
Juvenile Hormone biosynthesis and utilization of farnesoic acid during the final larval stadium of the ring‐legged earwig
Authors: III JOHN G. LUNZ , RANKIN SUSAN M.
Published: March 2008

Abstract. The regulation of Juvenile Hormone (JH) HI biosynthesis and release by the corpora allata (CA) was studied in final instar male and female larvae of the earwig, Euborellia annulipes, using a radiochemical assay in vitro. In males, maximal biosyntiiesis of JH IH occurred on day 1, then declined to virtually undetectable levels for the following 12 days of the stadium, and finally increased on days 14–16. In females, peaks of biosynthesis were detected on days 0–1 and on day 12. A further investigation of the 12‐day‐old larvae demonstrated mat in nonmoulting males and females, JH UJ biosynthesis was undetectable. However, for males and females undergoing ecdysis, the biosynthesis of JH III was detected and quantified.

The addition of 60 μM farnesoic acid to the incubation medium significantly increased the production of JH III by CA taken from females from day 8 until the end of the stadium. Glands from 12‐day old females that had initiated ecdysis were stimulated by farnesoic acid. By contrast, we could detect no stimulation of production of JH III by farnesoic acid in CA taken from males, even very late in the stadium. CA from newly emerged adult males and females were more active than those of larvae, and were greatly stimulated by farnesoic acid. CA from females immediately after emergence were stimulated significantly more by farnesoic acid man were glands from newly emerged males. These results suggest fundamental differences in the synmetic activity of CA for males and females in this insect.
Effects of carbon dioxide, acetone and 1‐octen‐3‐ol on the flight responses of the stable fly, Stomoxys calcitrans, in a wind tunnel
Authors: SCHOFIELD STEVEN , BRADy JOHN
Published: March 2008

Abstract. The flight behaviour of Stomoxys calcitrans (L.) in odour plumes containing carbon dioxide, acetone or l‐octen‐3‐ol was assessed from video recordings. A downwind bias was evident in clean air, whereas all three test chemicals elicited upwind anemotaxis. Response thresholds were ∼0.006% for CO2, between 0.001 and 0.01 μg/l for acetone, and ∼0.0002 u.g/1 for l‐octen‐3‐ol. Sinuosity (° cm‐1) and angular velocity (° s‐1) increased with C02 concentration, but velocity (cm s“1) decreased. Similar, but less clear effects were observed for acetone and l‐octen‐3‐ol.
Monoterpenes emitted by the large pine weevil, Hylobius abietis (L.) feeding on Scots pine, Pinus sylvestris L.
Authors: ZAGATTI PIERRE , LEMPERIERE GUy , MALOSSE CHRISTIAN
Published: March 2008

Abstract. Attraction of the large pine weevil, Hylobius abietis (L.) (Coleoptera: Curculionidae) to pine logs was enhanced by the presence of weevils already on the logs. We investigated the volatiles emitted by insects feeding on host trees to determine the mechanism of the attraction. Chemical analyses of the collected volatiles showed large amounts of monoterpenes. There were no qualitative differences in the collections, between Scots pine alone and pine plus feeding weevils, nor between males and females, mated or virgin. Strong quantitative differences in monoterpenes existed that were correlated to the surface of cut bark. Electroantennograms (EAGs) were recorded on male and female antennae stimulated by increasing doses of collected volatiles. EAGs for pine alone and pine plus weevils at the same stimulus loading in (a + P)‐pinene gave superimposable curves. EAGs of male and female volatiles were also identical. These results suggest an attraction mediated by host plant allelochemicals rather than by an aggregation pheromone.
New subspecies‐specific polymerase chain reaction‐based assay for the detection of Acidovorax avenae subsp. citrulli
Authors: Bahar O. , Efrat M. , Hadar E. , Dutta B. , Walcott R. R. , Burdman S.
Published: March 2008

New subspecies‐specific primers for the detection of Acidovorax avenae subsp. citrulli (Aac), the seedborne bacterium which causes bacterial fruit blotch of cucurbits, were designed based on PCR fragments obtained in ERIC‐ and BOX‐PCR profiles of Aac strains. PCR with both primer sets identified 30 strains from different locations and hosts, but did not amplify DNA from other closely related species and subspecies assessed, with the exception of DNA of one strain of A. avenae subsp. avenae, which was amplified by one of the primer sets, named BX‐S. This primer set, based on a BOX‐PCR fragment, performed under high‐stringency conditions without losing its detection sensitivity. The primers were also evaluated for their ability to detect the pathogen in contaminated watermelon and melon seed samples. The BX‐S primers facilitated the detection of the pathogen from washings of 5000‐seed samples with 0·02% infestation. This primer set was also assessed for detection using immunomagnetic separation polymerase chain reaction (IMS‐PCR) and was shown to be as sensitive as a previously described primer set (AACF2/R3), detecting 0·02% infestation in seed samples. This highly specific and sensitive primer set could be used to improve PCR‐based detection of this important pathogen.
Mefenoxam sensitivity and fitness analysis of Phytophthora nicotianae isolates from nurseries in Virginia, USA
Authors: Hu J. H. , Hong C. X. , Stromberg E. L. , Moorman G. W.
Published: March 2008

Mefenoxam is one of the most commonly used fungicides for managing diseases caused by Phytophthora spp. on ornamentals. The objectives of this study were to determine whether Phytophthora nicotianae, a destructive pathogen of numerous herbaceous annual and perennial plant species in nurseries, has developed resistance to mefenoxam, and to evaluate the fitness of mefenoxam‐resistant isolates. Ninety‐five isolates of P. nicotianae were screened for sensitivity to mefenoxam on 20% clarified V8 agar at 100 a.i. µg mL−1. Twenty‐five isolates were highly resistant to this compound with EC50 values ranging from 235·2 to 466·3 µg mL−1 and four were intermediately resistant with EC50 values ranging from 1·6 to 2·9 µg mL−1. Sixty‐six isolates were sensitive with EC50 values less than 0·04 µg mL−1. Nine resistant and seven sensitive isolates were tested for mefenoxam sensitivity on Pelargonium × hortorum cv. White Orbit. Mefenoxam provided good protection of pelargonium seedlings from colonization by sensitive isolates, but not by any resistant isolates. Four resistant and four sensitive isolates were compared for fitness components and their relative competitive ability on Lupinus Russell Hybrids in the absence of mefenoxam. Resistant isolates outcompeted sensitive ones within 3 to 6 sporulation cycles on lupin seedlings, regardless of their initial proportions in mixed zoospore inoculum. Resistant isolates exhibited greater infection rate and higher sporulation ability than sensitive ones when they were applied separately onto lupins. These results suggest that fungicide resistance may pose a serious challenge to the continued effectiveness of mefenoxam as a control option for nursery growers.
Identification of indole glucosinolate breakdown products with antifeedant effects on Myzus persicae (green peach aphid)
Authors: Kim Jae Hak , Lee Byong Won , Schroeder Frank C. , Jander Georg
Published: March 2008

The cleavage of glucosinolates by myrosinase to produce toxic breakdown products is a characteristic insect defense of cruciferous plants. Although green peach aphids (Myzus persicae) are able to avoid most contact with myrosinase when feeding from the phloem of Arabidopsis thaliana, indole glucosinolates are nevertheless degraded during passage through the insects. A defensive role for indole glucosinolates is suggested by the observation that atr1D mutant plants, which overproduce indole glucosinolates, are more resistant to M. persicae, whereas cyp79B2 cyp79B3 double mutants, which lack indole glucosinolates, succumb to M. persicae more rapidly. Indole glucosinolate breakdown products, including conjugates formed with ascorbate, glutathione and amino acids, are elevated in the honeydew of M. persicae feeding from atr1D mutant plants, but are absent when the aphids are feeding on cyp79B2 cyp79B3 double mutants. M. persicae feeding from wild‐type plants and myrosinase‐deficient tgg1 tgg2 double mutants excrete a similar profile of indole glucosinolate‐derived metabolites, indicating that the breakdown is independent of these foliar myrosinases. Artificial diet experiments show that the reaction of indole‐3‐carbinol, a breakdown product of indol‐3‐ylmethylglucosinolate, with ascorbate, glutathione and cysteine produces diindolylmethylcysteines and other conjugates that have antifeedant effects on M. persicae. Therefore, the post‐ingestive breakdown of indole glucosinolates provides a defense against herbivores such as aphids that can avoid glucosinolate activation by plant myrosinases.
Heteromeric K+ channels in plants
Authors: Lebaudy Anne , Hosy Eric , Simonneau Thierry , Sentenac Hervé , Thibaud Jean‐Baptiste , Dreyer Ingo
Published: March 2008

Voltage‐gated potassium channels of plants are multimeric proteins built of four α‐subunits. In the model plant Arabidopsis thaliana, nine genes coding for K+ channel α‐subunits have been identified. When co‐expressed in heterologous expression systems, most of them display the ability to form heteromeric K+ channels. Till now it was not clear whether plants use this potential of heteromerization to increase the functional diversity of potassium channels. Here, we designed an experimental approach employing different transgenic plant lines that allowed us to prove the existence of heteromeric K+ channels in plants. The chosen strategy might also be useful for investigating the activity and function of other multimeric channel proteins like, for instance, cyclic‐nucleotide gated channels, tandem‐pore K+ channels and glutamate receptor channels.
Generation of a 3D indexed Petunia insertion database for reverse genetics
Authors: Vandenbussche Michiel , Janssen Antoine , Zethof Jan , Van Orsouw Nathalie , Peters Janny , Van Eijk Michiel J.T. , Rijpkema Anneke S. , Schneiders Harrie , Santhanam Parthasarathy , De Been Mark , Van Tunen Arjen , Gerats Tom
Published: March 2008

BLAST searchable databases containing insertion flanking sequences have revolutionized reverse genetics in plant research. The development of such databases has so far been limited to a small number of model species and normally requires extensive labour input. Here we describe a highly efficient and widely applicable method that we adapted to identify unique transposon‐flanking genomic sequences in Petunia. The procedure is based on a multi‐dimensional pooling strategy for the collection of DNA samples; up to thousands of different templates are amplified from each of the DNA pools separately, and knowledge of their source is safeguarded by the use of pool‐specific (sample) identification tags in one of the amplification primers. All products are combined into a single sample that is subsequently used as a template for unidirectional pyrosequencing. Computational analysis of the clustered sequence output allows automatic assignment of sequences to individual DNA sources. We have amplified and analysed transposon‐flanking sequences from a Petunia transposon insertion library of 1000 individuals. Using 30 DNA isolations, 70 PCR reactions and two GS20 sequencing runs, we were able to allocate around 10 000 transposon flanking sequences to specific plants in the library. These sequences have been organized in a database that can be BLAST‐searched for insertions into genes of interest. As a proof of concept, we have performed an in silico screen for insertions into members of the NAM/NAC transcription factor family. All in silico‐predicted transposon insertions into members of this family could be confirmed in planta.
Interplay of miR164, CUP‐SHAPED COTYLEDON genes and LATERAL SUPPRESSOR controls axillary meristem formation in Arabidopsis thaliana
Authors: Raman Smita , Greb Thomas , Peaucelle Alexis , Blein Thomas , Laufs Patrick , Theres Klaus
Published: March 2008

Aerial architecture in higher plants is established post‐embryonically by the inception of new meristems in the axils of leaves. These axillary meristems develop into side shoots or flowers. In Arabidopsis, the NAC domain transcription factors CUP SHAPED COTYLEDON1 (CUC1), CUC2 and CUC3 function redundantly in initiating the shoot apical meristem and establishing organ boundaries. Transcripts of CUC1 and CUC2 are targeted for degradation by miR164. In this study, we show that cuc3‐2 mutants are impaired in axillary meristem initiation. Overexpression of miR164 in the cuc3‐2 mutant caused an almost complete block of axillary meristem formation. Conversely, mir164 mutants and plants harbouring miR164‐resistant alleles of CUC1 or CUC2 developed accessory buds in leaf axils. Collectively, these experiments reveal that, in addition to CUC3, redundant functions of CUC1 and CUC2 as well as miR164 regulation are required for the establishment of axillary meristems. Studies on LAS transcript accumulation in mir164 triple mutants and cuc3‐2 plants overexpressing miR164 suggest that regulation of axillary meristem formation by miR164 is mediated through CUC1 and CUC2, which in turn regulate LAS.
The oomycete response gene LURP1 is required for defense against Hyaloperonospora parasitica in Arabidopsis thaliana
Authors: Knoth Colleen , Eulgem Thomas
Published: March 2008

LURP1 is a member of the LURP cluster and the PR1 regulon, two previously defined sets of co‐expressed Arabidopsis thaliana genes that share a pronounced upregulation in response to infections by the pathogenic oomycete Hyaloperonospora parasitica. LURP1 shows the most extreme transcriptional inducibility by H. parasitica of all LURP and PR1 regulon genes. Using insertion mutants we found that LURP1 is required for full basal defense to H. parasitica and resistance to this pathogen mediated by the R‐proteins RPP4 and RPP5. The LURP1 protein shows no obvious similarity to proteins of known molecular function. We identified a 39‐bp region of the LURP1 promoter that mediates reporter gene expression in response to H. parasitica and salicylic acid. This promoter region contains a W box motif, WLURP1, that interacts in vitro with nuclear factors producing two separate DNA‐binding patterns. WLURP1‐related sequences are strongly enriched in the promoters of the PR1 regulon, suggesting a role for this motif in the coordinated expression of genes inducible by H. parasitica and related defense conditions.
Excessive Trichilemmal Keratinisation (flame follicles) In Endocrine Skin Disorders Of The Dog
Authors: Scott Danny W.
Published: March 2008

Abstract— Excessive trichilemmal keratinisation (flame follicle formation) was observed in the skin biopsies from 61 of 403 (15.1 %) dogs with endocrine skin disease. Flame follicles occurred in dogs with hypothyroidism, hyperadrenocorticism, hyposomatotropism, Sertoli's cell neoplasm with feminisation and castration‐responsive dermatosis. Flame follicles appeared to be a specific histopathological indicator of endocrine skin disease, as they were not observed in the skin biopsies from 2,865 dogs with non‐endocrine skin disease. They do not, however, indicate a specific endocrinopathy.

Résumé— Une kératinisation pilo‐folliculaire excessive (follicules en “flamme”) a été observée sur les biopsies de peau provenant de 61 chiens sur 403 (15.1%) atteints de dermatose d'origine endocrinienne. Des follicules en “flamme” ont été notés chez des chiens souffrant d'hypothyroidie, d'hypercorticisme, d'hyposomatotropisme, de sertolinome avec féminisation et de dermatose répondant à la castration. Les follicules en “flamme” représentent un signe histologique spécifique d'une dermatose d'origine endocrinienne car, ils n'ont pas été observés sur les biopsies de peau provenant de 2,865 chiens atteints de dermatose d'origine non endocrinienne. Toutefois, ils n'indiquent pas une endocrinopathie particuliere.

Zusammenfassung— Eine exzessive trichilemmale Keratinisierung (Flammenfollikel) wurde bei Hautbiopsien von 61 von 403 (15,1%) Hunden mit endokrinen Hauterkrankungen festgestellt. Diese “Flammenfollikel” traten bei Hunden mit Hypothyrose, Hyperadrenocortizismus, Hyposomatotropismus, Sertolizelitumoren mit Feminisierung und bei durch Kastration zu beeinflussenden Dermatosen auf.

Sie scheinen ein spezifischer histopathologischer Indikator für endokrine Hauterkrankungen zu sein, da sie in Hautbiopsien von 2865 Hunden mit nicht‐endokrinen Hauterkrankungen nicht festgestellt werden konnten. Sie geben allerdings keinen Hinweis auf eine spezifische Endokrinopathie.
Food Allergy In Dogs And Cats. A Review and Report of 43 Cases
Authors: CARLOTTI DIDIER N. , REMY ISABELLE , PROST CHRISTINE
Published: March 2008

Abstract— The literature on food allergy in dogs and cats is reviewed and 33 cases in dogs and 10 cases in cats, seen in Aquitaine, France, are described. Clinical aspects, diagnosis and aetiology are emphasised and compared with published data.

Résumé— Cet article fait la synthèse des données bibliographiques concernant l'allergie alimentaire chez le chien et le chat. Puis les auteurs présentent 33 cas de la maladie chez le chien et 10 cas chez le chat, observés en Aquitaine (France). Les aspects cliniques, diagnostiques et étiologiques sont soulignés et comparés aux données de la littérature.

Zusammenfassung— In diesem Artikel wird ein Rückblick über die Futtermittel‐allergie bie Hund und Katze in der Literatur gegeben. 33 Fälle dieser Erkrankung bei Hunden und 10 bei Katzen in Aquitanien, Frankreich, werden beschrieben. Klinische Aspekte, Diagnostik und Ätiologie werden herausgestellt und mit den Literaturangaben verglichen.

Resumen  Este artículo presenta una revisión bibliográfica de la alergia alimentaria en el perro y en el gato. A continuación se presentan los datos clinicos de 33 casos de alergia alimentaria en perros y de 10 casos de alergia alimentaria en gatos, diagnosticados en Aquitania, Francia. Se describen los signos clinicos, el diagnóstico y la etiologia y se comparan éstos con los descritos en la bibliografia.
Canine Vogt‐Koyanagi‐Harada‐like Syndrome in Two Siberian Husky Dogs
Authors: VERCELLI ANTONELLA , TARAGLIO STEFANO
Published: March 2008

Abstract— A syndrome of uveitis, associated with depigmentation and erosion of areas of skin and mucous membrane on the head and, in one dog, on the scrotum, was observed by the authors in two male Siberian Huskies. These clinical signs are similar to the Vogt‐Koyanagi‐Harada syndrome in man. Histological examination of the affected skin revealed a lichenoid dermatitis of the upper dermis. Intensive topical therapy with mydriatic and cycloplegic drugs and corticosteroids coupled with systemic prednisolone and azathioprine were used to control the disease. Both subjects were monitored for 10 months and retained useful vision. The skin and oral lesions resolved.

Résumé— Un syndrome d'uvéite idiopathique, non traumatique, associée à une dépigmentation et une érosion de zones cutanée et muqueuse sur la tête et, chez un chien, sur le scrotum, a été observé par les auteurs sur deux mâles Siberian Huskies. Ces signes cliniques évoquent le syndrome de Vogt—Koyanagi—Harada de l'homme. Un examen histologique de la peau atteinte a montré l'existence d'une dermatite lichénoide du derme superficiel. Un traitement topique intensif par des médicaments mydriatiques et cyclopégiques, ausociéà l'administration systémique de prednisolone et d'azathioprine a été utilisé pour controler la maladie. Les 2 sujets ont été observés pendant 10 mois et ont retrouvé une vision utile. Les lésions cutanées et orales ont guéri.

Zusammenfassung— Bei zwei Husky‐Rüden wurden von dem Autoren ein Krankheitssyndrom aus idiopathischer, nicht‐traumatisch bedingter Uveitis, Depigmentierung einzelner Haut‐ und Schleimhautbezirke am Kopf und—bei einem Hund—am Skrotum festgestellt. Diese Symptome ähneln dem Vogt‐Koyanagi‐Harada‐Syndrom beim Menschen. Bei der histologischen Untersuchung von Biopsien der erkrankten Hautbezirke wurde eine lichénoide Dermatitis der oberen Dermisschichten diagnostiziert. Durch eine intensive örtliche Behandlung mit Mydriatica, Cycloplegica und Corticosteroiden in Kombination mit systemischer Prednisolon‐ und Azathioptringabe konnte die Erkrankung unter Kontrolle gehalten werden. Beide Patienten wurden 10 Monate überwacht und sahen zufriedenstellend aus. Die Veränderungen an Haut und Mundhöhle bildeten sich zurück.

Resumen  Los autores describen un síndrome de uveitis no traúmatica idiopática asociada a una despigmentación y erosión de áreas de la piel y de las membranas cutáneas de la cabeza, en un perro y del escroto en otro perro. Ambos animales pertenecían a la raza Siberian Husky. Estos signos clínicos se asemejan en los descritos en la síndrome de Vogt‐Koyanagi‐Harada de las personas. El examen histológico mostró la presencia de un infiltrado inflamatorio liquenoide en la dermis superficial. Para el tratamiento a la enfermedad se aplicaron corticosteroides y fármacos midriáticos y ciclopéjicos de forma tópica y prednisona de forma sistémica. Ambos animales fueron controlados durante diez meses y mantuvieron la visión. Las lesiones orales y cutáneas se resolvieron por completo.
Bilateral Pinnal Squamous Cell Carcinoma in a Dog with Chronic Otitis Externa
Authors: MILLER WILLIAM H. , SHANLEY KEVIN J.
Published: March 2008

Abstract—An aged dog with a chronic, bilateral, bacterial otitis externa developed bilateral squamous cell carcinomas of the pinna. The tumours were located where the pinna would cover the external auditory meatus and probably were induced by the chronic infection.

Résumé—Un chien agé souftrant d'otite externe bactérienne chronique bilatérale présenta un épithelioma spinocellulaire bilatéral des pavilions auriculaires. Les tumeurs étaient situées là où les pavilions auraient recourvert le méat auriculaire et furent sans doute provoqués par l'infection chronique.

Zusammenfassung—Ein alter Hund mit einer chronischen, bilateralen, bakteriell bedingten Otitis externa entwickelte Plattenepithelkarzinome en beiden Ohrmuscheln. Diese Tumore traten an der Pinna exakt an der Mündung des äußeren Gehörgangs auf, und sind möglicherweise durch die chronische Infektion induziert worden.

Resumen Un perro de edad avanzada que presentaba una otitis externa bilateral bacteriana crónica desarrolló unos carcinomas de células escamosas bilaterales del pabellón auditivo. Los tumores estaban localizados en la entrada del meato auditivo y probablemente fueron inducidos por la infección crónica.
Bilaterally Symmetric Alopecia Associated with an Adrenocortical Adenoma in a Pet Ferret
Authors: SCOTT DANNY W. , HARVEY H. JAY , YEAGER AMY E.
Published: March 2008

Abstract—Bilaterally symmetrical alopecia in association with an adrenocortical adenoma was diagnosed in a 5‐year‐old, spayed female ferret. The only other clinical abnormality was vulvar enlargement. Hematologic, serum biochemical, urologic, and basal serum Cortisol values were all within normal limits. Ultrasonography revealed a mass in the area of the right adrenal gland. Surgical removal of the right adrenal gland resulted in complete recovery. Histologic examination of the excised adrenal gland revealed an adrenocortical adenoma.

Résumé—Une alopécie bilatérale symétrique associée à un adénome surrénalien éété diagnostiquée chez un furret femelle castrée de 5 ans. La seule autre manifestation clinique était une hypertrophie vulvaire. Les résultats des examens hématologiques, urinaires, biochimiques et la concentration de cortisol basal étaient dans les limites des valeurs usuelles. L'examen échographique a révéle la présence d'une masse en région surrénale droite. L'excision chirurgicale de la surrénale droite a provoqué la rémission compléte des symptômes. L'examen histologique da la glande excisée a révélé l'existence d'un adénome.

Zusammenfassung—Bei einem 5 Jahre alten, weiblichen, kastrierten Frettchen wurde eine bilateral symmetrische Alopezie in Verbindung mit einem Nebennierenrindenadenom diagnostiziert. Das einzige zusätzliche Symptom bestand in der Vergrößerung der Vulva. Die Laborergebnisse der Hämatologie, Serumbiochemie und Urinuntersuchung sowie der Serumkortisolwert bewegten sich innerhalb physiologischer Grenzen. Mittels Ultraschalluntersuchung konnte ein Gebilde in der Gegend der rechten Nebenniere festgestellt werden. Die chirurgische Entfernung der rechten Nebenniere führte zur einer vollständigen Wiederherstellung. Bei der histologischen Untersuchung der entfernten Nebenniere wurde ein adrenokortikales Adenom festgestellt.

Resumen En un hembra hurón castrada de 5 anos se diagnosticó alopecia simétrica bilateral asociada con un adenoma adrenocortical. Clínicamente, solo se observó abnormalidad, la vulva aumentada de tamaño. El exámen hematológico, bioquímico, urológico y el valor del cortisol basal, estaban dentro del rango normal. Por medio de la ultrasongrafía se detectó una masa en el área de la glándula adrenal derecha. La extirpación quirurgica de la glándula afectada produjo una recuperación completa. La examinación histológica de la glándula demostró la presencia de un adenoma.
Necrolytic Migratory Erythema in a Dog with a Glucagon‐Secreting Endocrine Tumor
Authors: MILLER WILLIAM H. , ANDERSON WAYNE I. , McCANN JOSEPH P.
Published: March 2008

Abstract—A 5‐year‐old mongrel dog developed necrolytic migratory erythema secondary to a glucagon‐secreting α cell tumor. The dog was anemic, glucose intolerant, and hyperglucagonemic but liver function tests were within normal limits. Metastatic tumor cells were found in the liver but the primary tumor was not identified.

Résumé—Un chien croisé mâle de 5 ans a développé un Erythème nécrolytique migrant secondaire à un glucayonome. L'animal était anémié, présentait une intolérance au glucose et une hyperglucagonémie, avec des tests fontcionnels hépatiques dans les limites des valeurs usuelles. Des cellules métastaiques tumorales ont été retrouvées dans le foie, mais la tumeur primitive n'a pas été identifiée.

Zusamenfassung—Ein 5 Jahr alter Mischingshund entwickelte sekundär auf einen Glukagon‐sezernierenden Tumor ein nekroyltisches Erythema migrans. Das Tier war anämisch, Glukose‐intolerant und hyperglukagonämische; die Leberfunktionstests befanden sich jedoch innerhalb normaler Grenzwerte. Matastatische Tumorzellen waren in der Leber nachweisbar, der Primärtumor konnte jedoch nicht identifizert werden.

Resumen Un perro cruzado de 5 años desarrollo un eritema necrolítico migratorio, secundario a un tumor de celulas secretoras de glucógeno. El perro era anémico, presentaba intolerancia a la glucosa, e hiperglucogenia, pero los resultados de los tests que evaluaban la función hepática feuron normales. Se encontraron células tumorales metastásicas procedentes del hígado, pero el tumor primario no se identificó. Se encontraron células tumorales metastásicas procedentes del hígado, pero el tumor primario no se identificicó.
Footpad Hyperkeratosis in a Family of Dogues de Bordeaux
Authors: PARADIS MANON
Published: March 2008

Abstract— Footpad hyperkeratosis is reported in a male Dogue de Bordeaux dog and six of his offspring. In each dog, the lesions were noted by the owners before six months of age. The hyperkeratosis can be improved but not resolved by regular footsoaks in propylene glycol, and secondary bacterial infection can be controlled with antibiotic therapy. This appears to be a new autosomal genodermatosis, but the exact mode of inheritance is unknown.

Résumé— L'hyperkératose des coussinets plantaires chez un chien male de race Dogue de Bordeaux ainsi que chez six de ses chiots est rapportée. Dans chaque cas, les lésions ont été notées par le propriétaire avant l'âge de six mois. L'hyperkératose peut être améliorée mais non guéri par des bains de pieds réguliers dans du propylène glycol et l'infection bactérienne secondaire peut être controlée à l'aide d'une antibiothérapie orale. Ceci semble être une nouvelle génodermatose autosomique, toutefois le mode d'héridité exact n'est pas connu.

Zusammenfassung— Es wird über eine Hyperkératose der Pfotenballen bei einem Bordeauxdoggenrüden und bei sechs seiner Nachkommen berichtet. Bei jedem Tier wurden die Veränderungen vor dem Alter von 6 Monaten vom Besitzer bemerkt. Die Hyperkératose kann durch regelmäßige Pfotenbäder mit Propylenglykol gebessert, nicht aber geheilt werden. Die sekundäre bakterielle Infektion kann durch antibiotische Therapie kontrolliert werden. Bei dem vorliegenden Fall scheint es sich um eine neue autosomale Génodermatose zu handeln, der genaue Erbgang ist jedoch unbekannt.

Resumen  En un dogo de Burdeos macho y 6 cachorros de su camada se reportó hiperkeratosis plantar. En cada caso, las lesiones fueron observadas por a el dueño antes de los seis meses de edad. Baños regulares con propilen glicol pueden controlar la hiperkeratosis, pero no curarla y la infección secundaria causada por bacteria, es susceptible at tratamiento con antibióticos. Aparentemente esto es debido a un nueva genodermatosis autosómica, pero el mode exacto de transmisión hereditaria es desconocido.
Pemphigus Vulgaris Lacking Mucosal Involvement in a German Shepherd Dog: Possible Response to Heparin Therapy
Authors: OLIVRY THIERRY , IHRKE PETER J. , ATLEE BARBARA A.
Published: March 2008

Abstract— An 8 year old intact female German shepherd dog was presented with well demarcated symmetric facial erosions, ulcerations and crusting. Systemic illness was absent, and oral involvement occurred only late in the course of the disease. Skin biopsy specimens revealed suprabasal acantholysis primarily involving hair follicles and direct immunofluorescent testing showed intercellular deposition of IgG in the epidermis compatible with a diagnosis of pemphigus vulgaris. The clinical signs in this dog were not adequately controlled by immune suppression. Therapy with heparin alone, or combined with prednisolone, appeared to induce a transient remission.

Résumé— Une femelle Berger Allemand de 8 ans a été présentée pour des lésions faciales bien circonscrites de la face: érosions, ulcères et croutes. 11 n'y avait pas d'atteinte de l'état general et l'atteinte buccale n'est apparue qu'en fin d'évolution. L'examen de biopsies cutanées a révélé la presence d'une acantholyse suprabasale atteignant primitivement les follicules pileux et l'immunofluorescence directe un dépöt intercellulaire d'IgG au niveau de l'épiderme, compatibles avec un diagnostic de pemphigus vulgaire. Les symptómes n'ont pas pu être controlés avec une thérapeutique immunosuppressive. Un traitement à base d'héparine seule, ou associée à la prednisone, a permis d'obtenir une rémission transitoire.

Zusammenfassung— Eine 8 Jahre alte, unkastrierte Deutsche Schäferhündin wurde mit gut abgegrenzten symmetrischen Erosionen, Ulzera und Verkrustungen im Gesichtsbereich vorgestellt. Systemische Krankheitssymptome fehlten, Veränderungen im Mundhöhlenbereich traten nur spat im Krankheitsverlauf auf. Hautbiopsieproacn zeigten eine suprabasale Akantholyse, die hauptsächlich die Haarfollikel betraf, die direktc immunfluoreszenzuntersuchung ergab cine interzelluläre Ablagerung von IgG in der Epidermis: Befunde, die mit der Diagnose Pemphigus vulgaris überenstimmen. Die klinischen Symptome bei diesem Hund konnten durch immunsuppression nicht adaquat kontrolliert werden. Eine Behandlung ausschlicßlich mit Heparin oder Heparin in Kombination mit Prednisolon schien eine vorübergchende Abheilung zu induzieren.

Resumen  Una hembra intacta pastor alemán de 8 años fué presentada con una erosión facial simétrica, úlceras y costras. El animal no sufría enfermedad sistémica y las lesiones orales ocurieron en el curso tardío de la enfermedad. La biopsia de la piel reveló acantolisis suprabasal localizada fundamentalmente en los folículos pilosos, y un test de inmunofluorescencia directa demonstró deposición intercelular de Tg G en la epidermis, compatible con la diagnosis de Pemphigus vulgaris. El cuadro clínico de este animal no se controló por medio de terapia inmunosupresiva. El tratamiento con heparina sola, o combinada con prednisolona produjo una remisión transitoria.
Progressive Dermal Collagenosis of Male Miniature Swine
Authors: McEWEN BEVERLY J. E.
Published: March 2008

Abstract— A unique dermatosis of male miniature swine is described. The disease occurs in post‐pubertal pigs, and is characterized by symmetrical, indurated, plaques over the truncal region. Histologically, the dermis and panniculus are effaced by thick, interwoven bundles of collagen resulting in an absence of deep dermal elastin. Hypertrophy and hyperplasia of superficial dermal vessels, fibroblasts and fibrocytes are accompanied by perivascular infiltrates of lymphocytes, plasma cells and eosinophils. The pathogenesis of this unusual dermatosis, designated as “progressive dermal collagenosis of male miniature swine” is unknown.

Résumé— Une dermatose originale du cochon nain mâle est décrite. La maladie apparait chez des cochons post pubertaires, et est caractérisée par des plaques sur le tronc, indurées et symétriques. Histologliquement, le derme et le pannicule sont envahis par d‘épais falsceaux de collagène, entrainant l'absence d’élastine dermique. Les vaisseaux du derme superficiei sont hyperplasiés et hypertrophiés, des fibroblastes et des fibrocytes sont accompagnés d'un infiltrat inflammatoire périvasculaire de lymphocytes, éosinophiles et plasmocytes. La pathogénie de cette affection peu commune, appelée “collagénose dermique progressive du cochon nain mâle” est inconnue.

Zusammenfassung— Es wird eine einzigartige Hauterkrankung beim männlichen Miniaturschwein beschrieben. Die Krankheit tritt bei postpubertären Schweinen auf und wird durch symmetrische, indurierte Plagues im Rumpfbereich gekennzeichnet. Histologisch treten Dermis und Pannikulus gegenüber dicken, verflochtenen Kollagenbündeln in den Hintergrund, wodurch das tiefe, dermale Elastin verschwindet. Die Hypertrophie und Hyperplasie der oberflächlichen Hautgefäße, Fibroblasten und Fibrozyten wird von perivaskulären Infiltraten aus Lymphozyten, Plasmazellen und eosinophilen Granulozyten begleitet. Die Pathogenese dieser ungewöhnlichen Dermatose, die als “progressive dermale Kollagenose des männlichen Miniaturschweins” bezeichnet wird, ist unbekannt.

Resumen  El presente artículo es una descripción de una dermatosis única del macho cerdo miniatura. Le enfermedad aparece después de la pubertad, y se caracteriza por la aparición de placas simétricas sobre la región del tronco. El exámen histológico de la dermis y del tejido panicular revela la presencia de gruesas bandas de colágeno entrelazadas, lo cual tiene como resultado la ausencia de la capa de elastina profunda. La hipertrofia e hiperplasia de los vasos superficiales dérmicos, fibroblastos y fibrocitos, se ve acompañadas de infiltrados perivasculares de linfocitos, células plasmáticas y eosinófilos.

La patogénesis de esta rara dermatosis Ilamada ‘colagenosis dérmica progresiva del cardo macho miniatura’, es desconocida.
Anagen Defluxion in Two Horses
Authors: MILNE ELSPETH M. , ROWLAND ALAN C.
Published: March 2008

Abstract— Anagen defluxion is an unusual cause of non‐pruritic alopecia in many species. Two cases are described in horses, the onset coinciding with upper respiratory disease associated with pyrexia. Skin biopsies showed typical changes with follicles containing broken hairs of irregular shaft diameter. The alopecia resolved slowly without therapy.

Résumé— L'effluvium anagène est une cause rare d'alopécie non prurigineuse dans de nombreuses espèces. Deux cas ont été obserés chez des chevaux. Le début a coincidé avec une atteinte des voies respiratoires supérieures associées à une fièvre. Les biopsies cutanées ont révélé des lésions caractéristiques: follicules contenant des poils cassés de diamètre iérrgulier. L'alopécie s'est progressivement résorbée sans traitement.

Zusammenfassung— Anagen‐Deflux ist eine ungewöhnliche Ursache für eine nicht pruritische Alopezie bei vielen Spezies. Beim Pferd werden zwei Fälle beschrieben, wobei der Beginn mit einer fieberhaften Erkrankung der oberen Atemwege zusammenfällt. Hautbiopsien zeigten typische Veränderungen mit Follikeln, die abgebrochene Haare mit unregelmäßigem Schaftdurchmesser enthielten. Die Alopezie heilte langsam ohne Therapie ab.

Resumen  La defluxión de la fase anagenica del pelo es una rara causa de alopecia de tipo no pruriginoso en una variedad de especies. En este artículo se describe el caso de dos caballos, en los cuales el punto más agudo de la enfermedad coincide con afeccion de las vías respiratorias altas acompañada de fiebre. Las biopsias cutáneas demostraron la presencia de cambios típicos en los folículos pilosos que contenían pelos rotos o de diámetro irregular. La alopecia se resolvió de manera lenta sin ningun tipo de terapia.
Cutaneous Mastocytosis and Mucinosis with Gross Deformity in a Shar pei Dog
Authors: MADEWELL BRUCE R. , AKITA GEOFFREY Y. , VOGEL PETER
Published: March 2008

Abstract—An 8‐year‐old Chinese Shar pei dog was examined because of gross deformity of the hind limbs. The deforming soft tissue swellings were associated with mucinosis and mastocytosis.

Résumé—Un Shar Peï de 8 ans a été examiné pour une déformation au niveau des membres postérieurs. Les épaissiessements des déformations des tissus mous étaient associés à une mucinose et une mastocytose.

Zusammenfassung—Ein 8 Jahre alter Chinesischer Shar Pei wurde wegen makroskopischer Verformung der Hintergliedmaßen untersucht. Die deformierenden Weichteilschwellungen standen in Zusammenhang mit einer Muzinose und Mastozytose.

Resumen Un perro Shar pei de 8 años de edad se examinó, debido a la presencia de vastas deformidades de los cuartos traseros. La deformidad de los tejidos blandos se asoció con mastocitosis y mucinosis.
Corneocyte Counts for Evaluation of Antiseborrheic Shampoos in Dogs
Authors: GORDON JOHN G. , KWOCHKA KENNETH W.
Published: March 2008

Abstract— An objective method for measuring corneocyte numbers before and after antiseborrheic shampoo therapy was assessed in dogs. Corneocytes were collected from six skin regions on the dorsal trunk of nine colony‐raised beagles with clinically normal skin. Forty‐eight collection sites were randomly assigned a treatment with one of seven medicated shampoos or water alone; six additional sites served as non‐treated controls. Corneocytes were collected immediately before the first treatment, and 14 and 28 days after initiation of therapy with a 0.1% Tween 80 surfactant and a quantitative cup‐scrub technique. Pretreatment corneocyte counts were not significantly different among the nine dogs nor among the randomly assigned treatment and control sites (3.55 · 104 cells.cm‐2, SEM 0.17). Corneocyte counts increased in all treatment and control sites from days 0 to 14. Corneocyte counts were similar in the non‐treated and water‐treated sites from days 14 to 28. Corneocyte counts were not significantly different when the same treatments were compared on days 0, 14 and 28, or when different treatments were compared on the same day. The corneocyte collection technique used in this study proved to be a reliable method for assessing the rate of cell desquamation and cell surface cohesion in dogs.
Dystrophic (Dermolytic) Epidermolysis Bullosa in a Cat
Authors: WHITE STEPHEN D. , DUNSTAN ROBERT W. , OLIVRY THIERRY , NAYDAN DIANE K. , RICHTER KEITH
Published: March 2008

Abstract— Dystrophic epidermolysis bullosa was diagnosed in an 8‐month‐old male domestic shorthaired cat. Lesions noted were paronychia, ulcers on the palate, oropharynx, gums and tongue, as well as ulceration and crusts on the metacarpal, metatarsal and digital pads. Histology of lesions showed dermal‐epidermaj separation and ulceration. Immunohistochemistry showed collagen IV (characteristic of the lamina densa) located at the roof of the cleft at both the dermal‐epidermal and follicular‐dermal interfaces. Electron microscopy showed the dermal‐epidermal cleft to be sublamina densa.

Résumé— Une épidermolyse bulleuse dystrophíque a été diagnostiquée chez un chat européen à poils courts de huit mois. Les lésions étaient une paronychie, des ulcères du palai, de l'oropharynx, des gencives et de la langue ains que des ulcères et des croutes au niveau des métacarpes, métatarses et coussinets plantaires. L'examen histologique des lésions faiasait apparaitre des ulcérations et des disjonctions derme/épiderme. L'étude immunohistochimique a montré la présence de fibres de collagène IV (caractéristiques de la lamina densa) sur lew toit des bulles tant au niveau des interfaces derme/épiderme que derme/follicule. La microscopie électronique a montré que les séparations derme/épiderme se faisaient au niveau de la sublamina densa.

Zusammenfassung— Eine dystrophe Epidermolysis bullosa wurde bei einer achtjährigen kurzhaarigen männlichen hauskatze diagnostiziert. Die beschriebenen Veränderungen bestanden in Paronychie, Ulzera an Gaumen, Oropharynx, Gingiva und Zunge sowie Ulzerationen und Krusten an den metacarpalen, metatarsalen und digitalen Ballen. Die Histologie der Veränderungen zeigte dermal‐epidermale Trennungen und Ulzerationen. Mit der Immunhistochemie wurde Kollagen IV nachgewiesen (ein Kennzeichen der Lamina densa), das am Gewölbe der Spaltbildung sowohl an den derma‐epidermalen als auch an den follikulär‐dermalen Schnittstellen lokalisiert war. Die Elektronenmikroskopie zeigte, daß die dermal‐epidermale Spaltbildung die Sublamina densa darstellte.

Resumen  En un gato macho de ocho meses doméstico de pelo corto se diagnóstico epidermolisis distrófica bullosa. Las lesiones presentes fueron paroniquia, úlceras del paladar, orofaringe, encías y lengua, así como ulceraciones y costras en las regiones del metacarpo, metatarso, y almohadillas plantares. Las lesiones histologicas consistieron de separación y ulceración a nivel de la dermis y epidermis. Exámenes inmunohistoquímicos demostraron la presencia de colágeno IV (característico de la lámina densa), localizado en el techo de las indentactiones en ambas interfases, epidermo‐dérmica y foliculo‐dérmica. Por medio de microscopía electrónica se comprobo que las indentaciones de la dermis y epidermis consistían de sublámina densa.
Giant Cell Dermatosis in FeLV‐positive Cats
Authors: GROSS THELMA LEE , CLARK EDWARD G. , HARGIS ANN M. , HEAD LAURIE L. , HAINESH DEBORAH M.
Published: March 2008

Abstract— Giant cell dermatosisis is reported in six cats with evidence of concurrent feline leukemia virus (FeLV) infection. Scaling, crusting and alopecia, accompanied by pruritus, affected the head and/or face predominantly in most cats; generalized skin disease developed in one cat. Histologically, giant keratinocytes resembling syncytial cells were found in the epidermis and superficial hair follicles, and were accompanied by dyskeratosis, pustules and ulcers. Immunohistochemical staining revealed FeLV antigen in the giant cells and adjacent keratinocytes. FeLV infection may have led to neoplastic alteration of the keratinocytes through recombination with host oncogenes

Resumen  Este artículo se basa en seis gatos con dermatosis de las células gigantes infectados con el virus FeLV. Éstos animales presentaban escamas y costras, así como pérdida de pelo y picor en las regiones de la cabera y cara, predominantemente. Tan solo uno de los gatos desarrolló una dermatosis generalizada. En el exámen histológico se demostró la presencia de keratinocitos gigantes con semejonza a las células sinticiales localizados en la epidermis y folículos pilosos superficiales, acompañados de disqueratosis, pústulas y ülceras. Las finturas histo‐inmunológicas demostraron el antígeno FeLV en las células gigantes y keratinocitos adyacentes. La injeccion con el virus FeLV pudo haber conducido a la alteración neoplástica de los keratinocitos a través de la recombinación con los oncogenes del hospedador.
Clinical Picture and Antibody Response in Pigs Infected by Sarcoptes scabiei var, suis
Authors: BORNSTEIN SET , ZAKRISSON GÖRAN
Published: March 2008

Abstract— Clinical signs and antibody responses were observed in juvenile SPF‐pigs experimentally infected with Sarcoptes scabiei var. suis and in neonatal pigs born to a sow cronically infected with S. scabiei. Mange became evident after 2–3 weeks. Three SPF‐pigs, which were infected twice, developed clinical signs a few days earlier than three other SPF‐pigs infected once. Serum antibodies to S. scabiei were demonstrated by an ELISA, using an extract of S. scabiei var. vulpes as an antigen, 5–7 weeks after the experimental infection and significant levels of antibody to S. scabiei were seen during the acute and chronic phases of the disease. Pruritus was seen at 4 days of age in piglets born to the infected sow. Maternal antibodies to S. scabiei were demonstrated within 6 h reaching mean maximum levels between 24 and 48 h after birth.

Résumé— Les symptômes et la réponse anticorps chez des pores SPF infestés expérimentalement par Sarcoptes scabiei var. suis et des porcelets nouveaux nés dans une porcherie infectée chroniquement par Sarcoptes scabiei one étéétudiés. La gale est apparue au bout de deux à trois semaines. Trois pores SPF, qui ont été infectés deux fois, ont présenté des symptômes quelques jours avant trois porcelet SPF infectés une seule fois. Les anticorps spécifiques de Sarcoptes scabiei ont été mis en évidence par une technique EIA, utilisant comme antigène des extraits de Sarcoptes scabiei tar. vulpes, 5 à 7 semaines après l'inoculmation. Des taux significatifs ont été observés tant pendant la phase aigue aue la phase chronique de la maladie. Le prurit était observé dès l'âge de 4 jours pour les porcelets nés dans la porcherie infectée. Des anticorps maternels spécifiques Sarcoptes scabiei ont été mis en evidence dès la sixième heure, avec des pics maximum entre 24 et 48 heures après la mise‐bas.