bluuebunny/crossref_metadata_2025 huggingface dataset:
Community Composition of Alpine Dung Beetles Is Mostly Driven by Temperature and Habitat Type
Authors: Laini Alex , Roggero Angela , Carlin Mario , Palestrini Claudia , Rolando Antonio
Published: August 2024

In alpine environments, open habitats alternate with wood to create a habitat mosaic that shapes insect community composition and diversity. Dung beetles are an important group of insects specialized in feeding on vertebrate dung whose availability also depends on habitat type. Although the habitat preferences of dung beetles have been extensively studied, few studies have addressed the influence of habitat structure and temperature on dung beetle communities in alpine environments. We sampled dung beetles in pastures, at the edges between pasture and wood, in inner and outer woods of two alpine areas at different altitudes (two sites per area). We found that pastures had higher mean temperatures compared to other habitat types and hosted the highest number of species. However, the interaction between habitat and altitude significantly affected species richness and abundance, suggesting that habitat type and temperature modulate the response of dung beetles in the study area. Edges hosted intermediate communities between pastures and woodlands and were populated by both pasture and woodland specialists. Our results suggest that maintaining pastures is crucial to preserving dung beetle communities.
Enumeration Agar, Acid Exposure and Sampling Time Are Relevant Factors Accounting for the High-Pressure Inactivation of Vegetative Pathogens in Fruit Puree
Authors: Torrents-Masoliver Berta , Jofré Anna , Ribas-Agustí Albert , Bover-Cid Sara
Published: August 2024

High pressure processing (HPP) is a non-thermal technology with emerging application within the fruit and vegetable sector. The impact of the enumeration agar on the recorded HPP inactivation of L. monocytogenes, Salmonella spp. and E. coli in banana–apple and apple purees was evaluated. Additionally, the HPP inactivation and sublethal injury was quantified in apple puree, considering the impact of acid exposure (24 h before HPP) and sampling time. Inoculated purees were pressurized at 300 MPa for 2 min. Enumeration was performed immediately and 24 h after HPP. HPP inactivation was 0.9-to-4.5-fold higher in apple than banana–apple puree. Compared with nutrient-rich media, selective agar enumeration overestimated the inactivation. HPP inactivation and sublethal injury of L. monocytogenes, Salmonella and E. coli was variable, mainly dependent on the exposure to acid and the sampling time. The 24 h-delayed enumeration slightly increased the inactivation. In apple puree, the CECT5947 strain of E. coli O157:H7 was the most piezo-resistant strain (1.5 log reduction), while L. monocytogenes Scott A was the most piezo-sensitive (6-log reduction when exposed to acid and sampled 24 h after HPP). All the studied factors should be taken into account when designing HPP treatments, performing product-specific validation studies and setting verification procedures.
Evaluating Biocompounds in Discarded Beetroot (Beta vulgaris) Leaves and Stems for Sustainable Food Processing Solutions
Authors: Mella Carolina , Rojas Natalia , Calderon-Bravo Hector , Muñoz Loreto A.
Published: August 2024

The current trend focuses on reducing food waste, with scientific studies exploring the nutritional value of discarded food components to identify potential health benefits. Beetroot (Beta vulgaris L.) is highly consumed, but its stems and leaves are often discarded. This work aims to characterize the chemical properties and bioactive compounds in beet stems and leaves and assess their applicability in food products. The stems and leaves were subjected to different drying temperatures (50 to 70 °C) to determine the optimal temperature for preserving their bioactive compounds. They are then nutritionally and physiochemically characterized and incorporated into a food matrix. The optimal drying temperature was 60 °C. The leaves and stems contain approximately 30 and 15 g/100 g of protein, 30 and 32 g/100 g of dietary fiber, 4 and 0.45 g/100 g of lipids, and 24 and 25 g/100 g of ash, respectively. Both provide approximately 50% of the amino acid requirements established by the WHO/FAO/UNU and are rich in iron and potassium. The stems presented 53% more betalainic compounds (0.58 mg/g) and a higher nitrate content (359 mg/kg) than did the leaves, which presented a higher polyphenol content. The incorporation of flour from beet stems and leaves into food products is economical, reduces food waste, and enhances nutrition and health.
Association between the Kihon Checklist and Working Status among Young-Old Citizens: A Cross-Sectional Study
Authors: Ohsugi Hironori , Anzai Saori , Shiba Yoshitaka
Published: August 2024

Owing to increasing super-aging societies, older adults will be encouraged to continue working. Although demand exists for older adults to continue working in Japanese society, the enabling factors have not been clarified. This study aimed to clarify (1) the life functions that affect the working status among younger-older adults who continue to work and those who do not via the Kihon Checklist (KCL) and (2) examine whether the number of areas of difficulties in daily functions of the KCL affected older adults’ employment status. This cross-sectional study involved 5386 older men and women aged 65 years or older in one city in Japan. Employment status and the seven domains of the Kihon Checklist (KCL) were analyzed. The KCL items related to employment status were the physical (odds ratio = 2.46, p < 0.01), socialization (odds ratio = 1.95, p < 0.01), and mood domains (odds ratio = 1.29, p < 0.01). Furthermore, the odds ratio increased to 2.06 when three or more domains were applicable. To remain employed, one must be physically and mentally healthy. Furthermore, since the risk of non-employment increased when one KCL domain was applicable, a broader assessment of life functions is necessary.
Transcendence in Molefi Kete Asante’s Afrocentricity and Tu Wei-ming’s Embodied Confucianism from the Perspective of Cultural Community
Authors: Zhou Yingli , Calloway-Thomas Carolyn , Li Gaowei
Published: August 2024

The concept of cultural community has been firstly or more obviously embodied in the works of the minority/minoritized literature or writers from marginalized countries and approached from different perspectives, such as small and enduring spiritual bonds, aspiration and an ideal, or self-deconstruction due to heterogeneity, conflict, and difference. However, most researchers explore the cultural community in the works of merely one racial group, such as American Indian, Chinese, Korean, or African. There has been comparatively little research on the construction of a cultural community across races. Focusing on Molefi Kete Asante’s Afrocentricity and Tu Wei-ming’s embodied Confucianism, two cultural movements that fully embody a “new cosmopolitanism” and have the potential to dialog and complement each other, this study compares the views of transcendence of these two philosophies in terms of sense, the ultimate goal, orientation of time, vehicle for realization, and thinking pattern in the hope of the construction of a Sino-African cultural community, which reflects mutual understanding, coexistence, harmony without uniformity, and the contact, conflict, and intermingling of heterogeneous cultures.
Positive Association between Patients’ Perception of Chronic Pain Rehabilitation as a Personally Meaningful Experience and the Flourishing Aspect of Well-Being
Authors: Liddiard Katrina J. , Brown Cary A. , Raynor Annette J.
Published: August 2024

Chronic pain rehabilitation helps to reduce pain and restore valued life roles. Patients may have more positive outcomes when they perceive rehabilitation to be personally meaningful. This study examined associations between self-reported, personally meaningful rehabilitation and well-being. A pilot study was conducted using an online survey of people with chronic pain and experiences of rehabilitation. The PROMIS Pain Interference Short Form 8a and The Flourishing Scale were used to explore well-being. A modified self-report measure, the Meaningfulness in Rehabilitation Scale, was pilot-tested for construct validity and used in the survey. Of the 48 participants (81% female; 19% male), most attended a generalist therapy practice (62%) once per week (33%) or once per fortnight (29%). No statistically significant relationship was found between self-reported meaningfulness in rehabilitation and pain interference or other patient and therapy characteristics (duration of chronic pain category, type of therapy practice, resolution of rehabilitation category, and frequency of appointments). The nonparametric analysis identified a statistically significant moderate positive correlation between self-reported meaningfulness in rehabilitation and the flourishing aspect of well-being. This raises important questions and suggests that patients’ perception of rehabilitation as meaningful warrants further research. This pilot study provides valuable guidance to inform a larger investigation.
Could 101-14 Mgt Rootstock Affect Post-Spring Frost Vine Developing? Preliminary Findings
Authors: Gutiérrez-Gamboa Gastón , Palacios-Peralta Cristóbal , Verdugo-Vásquez Nicolás , Reyes-Díaz Marjorie , Muñoz Ariel , Ribera-Fonseca Alejandra
Published: August 2024

(1) Background: Spring frost damage is a common phenomenon that occurs in Southern Chile that considerably affects vine productivity and grape quality. (2) Methods: A field trial was conducted in order to study vine phenology and berry physicochemical parameters in Chardonnay, Sauvignon Blanc and Pinot Noir ungrafted and grafted (onto 101-14 Mgt rootstock) grapevines after a spring frost. This event killed the totality of primary bud shoots when the vines reached the phenological stage of unfolded leaves. (3) Results: From budburst, to flowering of secondary bud shoots, ungrafted Sauvignon Blanc grapevines presented an advanced phenology, whereas 101-14 Mgt rootstock tended to advance the maturity of Pinot Noir grapevines from flowering to ripening of berries. At harvest, berries from secondary buds of vines grafted onto 101-14 Mgt rootstock showed higher soluble solids than the ones from ungrafted Chardonnay and Sauvignon Blanc vines. High total phenolic content was found in berries from secondary buds of the grafted vines, compared to the ones from the ungrafted vines. Berry soluble solids variability tended to statistically decrease toward harvest in the studied plant materials, and the maximum coefficient of variation for soluble solids, berry weight, berry firmness and berry size reached 9.5%, 25.9%, 18.6% and 8.9%, respectively. (4) Conclusions: These preliminary results may be interesting for the Southern Chilean viticulturists since it seems that 101-14 Mgt rootstock could affect phenology and grape berry maturity of grapevines established in the Cautín Valley after spring frost damage.
The Roles of Obesity and ASB4 in Preeclampsia Pathogenesis
Authors: Wang Yuye , Ssengonzi Rebecca , Townley-Tilson W. H. Davin , Kayashima Yukako , Maeda-Smithies Nobuyo , Li Feng
Published: August 2024

Preeclampsia is a complex pregnancy-related hypertensive disorder which poses significant risks for both maternal and fetal health. Preeclampsia affects 5–8% of pregnancies in the United States, causing a significant public health and economic burden. Despite extensive research, the etiology and pathogenesis of preeclampsia remain elusive, but have been correlated with maternal conditions such as obesity. In recent decades, the incidence of preeclampsia increased along with the prevalence of obesity among women of reproductive age. Maternal obesity has been shown to negatively affect pregnancy in almost all aspects. However, the precise mechanisms by which obesity influences preeclampsia are unclear. Ankyrin repeat and SOCS Box Containing protein 4 (ASB4) is an E3 ubiquitin ligase that can promote the degradation of a wide range of target proteins. ASB4-null mice display a full spectrum of preeclampsia-like phenotypes during pregnancy including hypertension, proteinuria, and decreased litter size. Furthermore, maternal obesity induced by a high-fat diet aggravates preeclampsia-like phenotypes in pregnant mice lacking ASB4. Variants in the ASB4 gene have been associated with obesity in humans, and a functional connection between the ASB4 gene and obesity has been established in mice. This review discusses the connections between preeclampsia, obesity, and ASB4.
A Fungicide, Fludioxonil, Formed the Polyploid Giant Cancer Cells and Induced Metastasis and Stemness in MDA-MB-231 Triple-Negative Breast Cancer Cells
Authors: Go Ryeo-Eun , Seong Su-Min , Choi Youngdong , Choi Kyung-Chul
Published: August 2024

Fludioxonil, an antifungal agent used as a pesticide, leaves a measurable residue in fruits and vegetables. It has been identified to cause endocrine disruption, interrupt normal development, and cause various diseases such as cancers. In this study, fludioxonil was examined for its effects on the development and metastasis of breast cancer cells. On fludioxonil exposure (10−5 M) for 72 h, mutant p53 (mutp53) MDA-MB-231 triple-negative breast cancer (TNBC) cells significantly inhibited cell viability and developed into polyploid giant cancer cells (PGCCs), with an increase in the number of nuclei and expansion in the cell body size. Fludioxonil exposure disrupted the normal cell cycle phase ratio, resulting in a new peak. In addition, PGCCs showed greater motility than the control and were resistant to anticancer drugs, i.e., doxorubicin, cisplatin, and 5-fluorouracil. Cyclin E1, nuclear factor kappa B (NF-κB), and p53 expressions were remarkably increased, and the expression of cell cycle-, epithelial–mesenchymal-transition (EMT)-, and cancer stemness-related proteins were increased in the PGCCs. The daughter cells obtained from PGCCs had the single nucleus but maintained their enlarged cell size and showed greater cell migration ability and resistance to the anticancer agents. Consequently, fludioxonil accumulated Cyclin E1 and promoted the inflammatory cytokine-enriched microenvironment through the up-regulation of TNF and NF-κB which led to the transformation to PGCCs via abnormal cell cycles such as mitotic delay and mitotic slippage in mutp53 TNBC MDA-MB-231 cells. PGCCs and their daughter cells exhibited significant migration ability, chemo-resistance, and cancer stemness. These results strongly suggest that fludioxonil, as an inducer of potential genotoxicity, may induce the formation of PGCCs, leading to the formation of metastatic and stem cell-like breast cancer cells.
Antioxidant Role of Probiotics in Inflammation-Induced Colorectal Cancer
Authors: Hamamah Sevag , Lobiuc Andrei , Covasa Mihai
Published: August 2024

Colorectal cancer (CRC) continues to be a significant contributor to global morbidity and mortality. Emerging evidence indicates that disturbances in gut microbial composition, the formation of reactive oxygen species (ROS), and the resulting inflammation can lead to DNA damage, driving the pathogenesis and progression of CRC. Notably, bacterial metabolites can either protect against or contribute to oxidative stress by modulating the activity of antioxidant enzymes and influencing signaling pathways that govern ROS-induced inflammation. Additionally, microbiota byproducts, when supplemented through probiotics, can affect tumor microenvironments to enhance treatment efficacy and selectively mediate the ROS-induced destruction of CRC cells. This review aims to discuss the mechanisms by which taxonomical shifts in gut microbiota and related metabolites such as short-chain fatty acids, secondary bile acids, and trimethylamine-N-oxide influence ROS concentrations to safeguard or promote the onset of inflammation-mediated CRC. Additionally, we focus on the role of probiotic species in modulating ROS-mediated signaling pathways that influence both oxidative status and inflammation, such as Nrf2-Keap1, NF-κB, and NLRP3 to mitigate carcinogenesis. Overall, a deeper understanding of the role of gut microbiota on oxidative stress may aid in delaying or preventing the onset of CRC and offer new avenues for adjunct, CRC-specific therapeutic interventions such as cancer immunotherapy.
Synthesis of Natural and Sugar-Modified Nucleosides Using the Iodine/Triethylsilane System as N-Glycosidation Promoter
Authors: Cimafonte Martina , Esposito Anna , De Fenza Maria , Zaccaria Francesco , D’Alonzo Daniele , Guaragna Annalisa
Published: August 2024

The reagent system based on the combined use of Et3SiH/I2 acts as an efficient N-glycosidation promoter for the synthesis of natural and sugar-modified nucleosides. An analysis of reaction stereoselectivity in the absence of C2-positioned stereodirecting groups revealed high selectivity with six-membered substrates, depending on the nucleophilic character of the nucleobase or based on anomerization reactions. The synthetic utility of the Et3SiH/I2-mediated N-glycosidation reaction was highlighted by its use in the synthesis of the investigational drug apricitabine.
The Pathophysiological Mechanism and Clinical Treatment of Polycystic Ovary Syndrome: A Molecular and Cellular Review of the Literature
Authors: Chang Kai-Jung , Chen Jie-Hong , Chen Kuo-Hu
Published: August 2024

Polycystic ovary syndrome (PCOS) is a prevalent metabolic disorder among women of reproductive age, characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovaries. The pathogenesis of PCOS involves a complex interplay of genetic and environmental factors, including insulin resistance (IR) and resultant hyperinsulinemia. Insulin receptors, primarily in skeletal muscle, liver, and adipose tissue, activate downstream signaling pathways like PI3K-AKT and MAPK-ERK upon binding. These pathways regulate glucose uptake, storage, and lipid metabolism. Genome-wide association studies (GWASs) have identified several candidate genes related to steroidogenesis and insulin signaling. Environmental factors such as endocrine-disrupting chemicals and lifestyle choices also exacerbate PCOS traits. Other than lifestyle modification and surgical intervention, management strategies for PCOS can be achieved by using pharmacological treatments like antiandrogens, metformin, thiazolidinediones, aromatase inhibitor, and ovulation drugs to improve insulin sensitivity and ovulatory function, as well as combined oral contraceptives with or without cyproterone to resume menstrual regularity. Despite the complex pathophysiology and significant economic burden of PCOS, a comprehensive understanding of its molecular and cellular mechanisms is crucial for developing effective public health policies and treatment strategies. Nevertheless, many unknown aspects of PCOS, including detailed mechanisms of actions, along with the safety and effectiveness for the treatment, warrant further investigation.
Comparative Analysis of Water Hammer Performance in Different Pipe Parameters with FSI
Authors: Kandil Mostafa , El-Sayed Tamer A. , Kamal Ahmed M.
Published: August 2024

Water hammer (WH) is a critical phenomenon in fluid-filled piping systems that can lead to severe pressure surges and structural damage. The characteristics of the pipe material, geometry, and support conditions play a crucial role in the fluid–structure interaction (FSI) during WH events. This study investigates the impact of various pipe parameters, including material, length, thickness, and diameter, on the WH behavior using an FSI-based numerical approach. A comprehensive computational model was developed based on the algorithm presented in Delft Hydraulics Benchmark Problem (A) to simulate the WH phenomenon in pipes made of different materials, such as steel, copper, ductile iron, PPR (polypropylene random copolymer), and GRP (glass-reinforced plastic). This study examines the influence of pipe parameters on WH performance in pipelines, utilizing FSI to analyze the phenomenon. The results show that the pipe material has a significant influence on the pressure wave speed, stress wave propagation, and the overall system response during WH. Pipes with lower modulus of elasticity, such as PPR and GRP, exhibit lower pressure wave speeds but higher stress wave speeds compared with steel pipes. Increasing the elastic modulus, pipe wall thickness, length, and diameter enhances the pipe’s stiffness and impacts the timing, magnitude of pressure surges, and the likelihood of cavitation. The findings of this study provide valuable insights into the design and mitigation of WH in piping systems.
Role of Cathelicidins in Atherosclerosis and Associated Cardiovascular Diseases
Authors: Dabravolski Siarhei A. , Orekhov Nikolay A. , Churov Alexey V. , Starodubtseva Irina A. , Beloyartsev Dmitry F. , Kovyanova Tatiana I. , Sukhorukov Vasily N. , Orekhov Alexander N.
Published: August 2024

Cathelicidins (human LL-37 and rat CRAMP) are multifunctional peptides involved in various cardiovascular conditions. This review integrates the recent findings about the functional involvement of LL-37/CRAMP across atherosclerosis, acute coronary syndrome, myocardial infarction, heart failure, diabetic cardiomyopathy, and platelet aggregation/thrombosis. In atherosclerosis, LL-37 interacts with scavenger receptors to modulate lipid metabolism and binds with mitochondrial DNA and lipoproteins. In acute coronary syndrome, LL-37 influences T cell responses and mitigates calcification within atherosclerotic plaques. During myocardial infarction and ischaemia/reperfusion injury, LL-37/CRAMP exhibits dual roles: protecting against myocardial damage through the AKT and ERK1/2 signalling pathways, while exacerbating inflammation via TLR4 and NLRP3 inflammasome activation. In heart failure, LL-37/CRAMP attenuates hypertrophy and fibrosis via NF-κB inhibition and the activation of the IGFR1/PI3K/AKT and TLR9/AMPK pathways. Moreover, in diabetic cardiomyopathy, these peptides alleviate oxidative stress and fibrosis by inhibiting TGFβ/Smad and AMPK/mTOR signalling and provide anti-inflammatory effects by reducing NF-κB nuclear translocation and NLRP3 inflammasome formation. LL-37/CRAMP also modulates platelet aggregation and thrombosis through the FPR2 and GPVI receptors, impacting apoptosis, autophagy, and other critical cellular processes. This comprehensive overview underscores LL-37/CRAMP as a promising therapeutic target in cardiovascular diseases, necessitating further elucidation of its intricate signalling networks and biological effects for clinical translation.
Global Media Sentiments on the Rohingya Crisis: A Comparative Analysis of News Articles from Ten Countries
Authors: Al-Zaman Md. Sayeed , Rashid Mohammad Harun Or
Published: August 2024

The Rohingya crisis has been a significant issue for national and international news media, capturing their attention for an extended period and documenting various phases of the crisis. Previous research exploring the tones and portrayal of the Rohingyas in the news lacks comparative and temporal analysis of news sentiments. In this study, we aim to fill this gap by analyzing 8074 news stories on Rohingya issues published in 10 news media outlets from 10 countries between 2009 and 2023. Our computational sentiment analysis reveals that Rohingya-related news sentiments are predominantly negative and fluctuate over the years across different countries, showing little identifiable patterns. An ANOVA suggests significant variation in news sentiments among countries, with some countries exhibiting more similar sentiments than others, thus creating distinguishable groups. Some of our findings contradict previous scholarships, warranting further research and novel frameworks. Additionally, we encourage scrutiny of academic insights to address potential biases against news media’s journalistic integrity.
Spatial–Temporal Changes and Driving Factor Analysis of Net Ecosystem Productivity in Heilongjiang Province from 2010 to 2020
Authors: Zhang Hui , He Zhenghong , Zhang Liwen , Cong Rong , Wei Wantong
Published: August 2024

Net ecosystem productivity (NEP) is an important indicator for the quantitative evaluation of carbon sources/sinks in terrestrial ecosystems. An improved CASA model and soil respiration model, combined with MODIS and meteorological data, are utilized to estimate vegetation NEP from 2010 to 2020. A Theil–Sen trend analysis, a Mann–Kendall test, the Hurst index, and geographical detector methods were employed to analyze the spatiotemporal variations in NEP in Heilongjiang Province and its driving factors. The results show the following: (1) The overall NEP in Heilongjiang Province exhibited a fluctuating upward trend from 2010 to 2020, with a growth rate of 4.74 g C·m−2·yr−1, and an average annual NEP of 404 g C·m−2·yr−1. Spatially, NEP exhibits a distribution pattern of “low from east to west to high from north to south in the central region”, with 99.27% of the area being a carbon sink. (2) Significant regional differences were observed in the spatial trend of NEP changes, with 78.39% of regions showing increasing trends and 17.53% showing decreasing trends. Future NEP changes are expected to continue, with regions showing a persistent increase (58.44%), potential decrease (19.95%), potential increase (5.65%), and persistent decrease (11.88%). (3) The geographical detector results indicate that altitude is the dominant factor affecting NEP, followed by slope, temperature, population density, etc. The interaction-detector results show that the interaction between each factor shows an increasing trend, and the interaction between any two factors is higher than that of a single factor. The research results can provide scientific references for reducing emissions, increasing sinks, and protecting ecosystems in Heilongjiang Province.
Optimization of Foams—Polypropylene Fiber-Reinforced Concrete Mixtures Dedicated for 3D Printing
Authors: Rudziewicz Magdalena , Maroszek Marcin , Setlak Kinga , Góra Mateusz , Hebda Marek
Published: August 2024

The continued global urbanization of the world is driving the development of the construction industry. In order to protect the environment, intensive research has been carried out in recent years on the development of sustainable materials and ecological construction methods. Scientific research often focuses on developing building materials that are renewable, energy-efficient, and have minimal impact on the environment throughout their life cycle. Therefore, this article presents research results aimed at developing a concrete mixture using cement with reduced CO2 emissions. In the context of increasing ecological awareness and in line with European Union policy, the development of a mixture based on environmentally friendly cement is of key importance for the future development of the construction industry. The article compares the physical properties of two mixtures, their foaming possibilities, and the influence of the added polypropylene (PP) fibers on the strength properties of the produced composites. It was found that bending strength and compressive strength were highest in the material with silica fume and aluminum powder at 5.36 MPa and 28.76 MPa, respectively. Microscopic analysis revealed significant pore structure differences, with aluminum foamed samples having regular pores and hydrogen peroxide foamed samples having irregular pores. Optimizing aluminum powder and water content improved the materials’ strength, crucial for maintaining usability and achieving effective 3D printing. The obtained results are important in the development of research focused on the optimization of 3D printing technology using concrete.
Effect of Scaffold Geometrical Structure on Macrophage Polarization during Bone Regeneration Using Honeycomb Tricalcium Phosphate
Authors: Takabatake Kiyofumi , Tsujigiwa Hidetsugu , Nakano Keisuke , Chang Anqi , Piao Tianyan , Inada Yasunori , Arashima Takuma , Morimatsu Ayumi , Tanaka Ayumi , Kawai Hotaka , Nagatsuka Hitoshi
Published: August 2024

The polarization balance of M1/M2 macrophages with different functions is important in osteogenesis and bone repair processes. In a previous study, we succeeded in developing honeycomb tricalcium phosphate (TCP), which is a cylindrical scaffold with a honeycomb arrangement of straight pores, and we demonstrated that TCP with 300 and 500 μm pore diameters (300TCP and 500TCP) induced bone formation within the pores. However, the details of the influence of macrophage polarization on bone formation using engineered biomaterials, especially with respect to the geometric structure of the artificial biomaterials, are unknown. In this study, we examined whether differences in bone tissue formation due to differences in TCP geometry were due to the polarity of the assembling macrophages. Immunohistochemistry for IBA-1, iNOS, and CD163 single staining was performed. The 300TCP showed a marked infiltration of iNOS-positive cells, which are thought to be M1 macrophages, during the osteogenesis process, while no involvement of CD163-positive cells, which are thought to be M2 macrophages, was observed in the TCP pores. In addition, 500TCP showed a clustering of iNOS-positive cells and CD163-positive cells at 2 weeks, suggesting the involvement of M2 macrophages in the formation of bone tissue in the TCP pores. In conclusion, we demonstrated for the first time that the geometrical structure of the artificial biomaterial, i.e., the pore size of honeycomb TCP, affects the polarization of M1/2 macrophages and bone tissue formation in TCP pores.
Experimental Investigation and Bayesian Assessment for Permeability Characteristics of Lightweight Ceramsite Concrete
Authors: Li Min , Wang Yongjun , Chen Mengzhang , Zhang Lin , Xu Yinshan , Zhao Hongbo , Ren Jiaolong
Published: August 2024

Ceramsite concrete is one of the most widely used lightweight concretes at present. Although mechanical properties of ceramsite concrete have been extensively discussed, its permeability characteristics are neglected in previous studies. Considering the importance of permeability resistance to concrete, the permeability grade and residual compressive strength after permeability of ceramsite concrete are analyzed in this study. The influence of ceramsite content and size on the permeability grade and residual strength of ceramsite concrete were investigated by the orthogonal experimental method. To further understand the above influence, an improved Bayesian framework for small sample data is also established to analyze the permeability grade and residual strength. Results show that the water–binder ratio and the content of 20–30 mm ceramsite aggregates are the most and least significant influencing factors affecting the permeability characteristics, respectively. The 5–10 mm and 10–20 mm ceramsite aggregates play secondary roles. Increasing 5–10 mm and 10–20 mm ceramsite aggregates is not helpful for improving the permeability resistance of ceramsite concrete. Compared with the orthogonal method, the proposed Bayesian framework is a useful tool for revealing the effects of various factors, which can cut the time cost and provide parameter visualization for the analysis process. Results show that the permeability resistance and residual strength of ceramsite concrete are improved significantly under optimal conditions. The permeability grade and residual strength are increased 200% and 80.3%, respectively. In addition, the residual strength may be more suitable for evaluating the permeability characteristics than the permeability grade.
Seawater Mixed with One Part Alkali Activated Material: An Environmental and Cost Evaluation
Authors: Li Xiaoyu , Liu Huiyang , Xing Jinxin , Gan Min , Ji Zhiyun , Fan Xiaohui , Sun Zengqing
Published: August 2024

Concrete production is associated with extensive energy consumption and significant CO2 emissions. In addition, tremendous amounts of freshwater are used as a mixing agent. Urgency is increasing to develop sustainable cementitious materials and promote freshwater-saving strategies. An environmentally friendly alternative binder, seawater mixed with one part alkali activated material, is studied. In this work, a cradle-to-gate life cycle assessment was applied to study the equivalent CO2 emission and cost properties of the clinker-free binder. The seawater mixed mortar possesses comparable mechanical properties to Portland cement, with 3 d flexural and compressive strengths of 5.3 MPa and 25.2 MPa. In addition, the mortar developed in this work is of similar cost as commercial cement, but reduces CO2 emissions by 44.8%.
Hydroxyapatite-Based Natural Biopolymer Composite for Tissue Regeneration
Authors: Alkaron Wasan , Almansoori Alaa , Balázsi Katalin , Balázsi Csaba
Published: August 2024

Hydroxyapatite (HAp) polymer composites have gained significant attention due to their applications in bone regeneration and tooth implants. This review examines the synthesis, properties, and applications of Hap, highlighting various manufacturing methods, including wet, dry, hydrothermal, and sol–gel processes. The properties of HAp are influenced by precursor materials and are commonly obtained from natural calcium-rich sources like eggshells, seashells, and fish scales. Composite materials, such as cellulose–hydroxyapatite and gelatin–hydroxyapatite, exhibit promising strength and biocompatibility for bone and tissue replacement. Metallic implants and scaffolds enhance stability, including well-known titanium-based and stainless steel-based implants and ceramic body implants. Biopolymers, like chitosan and alginate, combined with Hap, offer chemical stability and strength for tissue engineering. Collagen, fibrin, and gelatin play crucial roles in mimicking natural bone composition. Various synthesis methods like sol–gel, hydrothermal, and solution casting produce HAp crystals, with potential applications in bone repair and regeneration. Additionally, the use of biowaste materials, like eggshells and snails or seashells, not only supports sustainable HAp production but also reduces environmental impact. This review emphasizes the significance of understanding the properties of calcium–phosphate (Ca-P) compounds and processing methods for scaffold generation, highlighting novel characteristics and mechanisms of biomaterials in bone healing. Comparative studies of these methods in specific applications underscore the versatility and potential of HAp composites in biomedical engineering. Overall, HAp composites offer promising solutions for improving patient outcomes in bone replacement and tissue engineering and advancing medical practices.
Conductive Polymer-Based Electrodes and Supercapacitors: Materials, Electrolytes, and Characterizations
Authors: Roohi Zahra , Mighri Frej , Zhang Ze
Published: August 2024

New materials and the interactions between them are the basis of novel energy storage devices such as supercapacitors and batteries. In recent years, because of the increasing demand for electricity as an energy source, the development of new energy storage materials is among the most actively studied topics. Conductive polymers (CPs), because of their intrinsic electrochemical activity and electrical conductivity, have also been intensively explored. While most of the high capacitance reported in the literature comes from hybrid materials, for example, conductive polymers composed of metal oxides and carbon materials, such as graphene and carbon nanotubes, new chemistry and the 3D structure of conductive polymers remain critical. This comprehensive review focuses on the basic properties of three popular conductive polymers and their composites with carbon materials and metal oxides that have been actively explored as energy storage materials, i.e., polypyrrole (PPy), polyaniline (PANi), and polythiophene (PTh), and various types of electrolytes, including aqueous, organic, quasi-solid, and self-healing electrolytes. Important experimental parameters affecting material property and morphology are also discussed. Electrochemical and analytical techniques frequently employed in material and supercapacitor research are presented. In particular, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) are discussed in detail, including how to extract data from spectra to calculate key parameters. Pros and cons of CP-based supercapacitors are discussed together with their potential applications.
The Influence of Various Modifications of Hazelnut Shell Flour as Potential Filler in Plywood Technology
Authors: Wronka Marta , Wojnicz Damian , Kowaluk Grzegorz
Published: August 2024

This study investigates the potential of utilizing hazelnut shells (HS) as an innovative filler in three-layer plywood technology, addressing the growing need for sustainable, high-performance materials. Traditional plywood production relies on adhesives enhanced with various fillers to improve physical, mechanical, and operational characteristics. This research explores using native, chemically modified, and activated carbon derived from hazelnut shells as fillers in urea–formaldehyde (UF) resin. The produced plywood’s mechanical properties, water absorption, and formaldehyde emissions were thoroughly analyzed. Key findings demonstrate that incorporating 10 part by weight (pbw) native hazelnut shell flour significantly enhances the modulus of rupture (MOR) to 138.6 N mm−2 and modulus of elasticity (MOE) to 13,311 N mm−2. Chemically modified hazelnut shell flour achieves optimal results at 5 pbw, while activated carbon from hazelnut shells, even at 1 pbw, markedly improves bonding strength (2.79 N mm−2 referred to 0.81 N mm−2 for reference sample without filler added). Notably, activated carbon effectively reduces formaldehyde emissions (2.72 mg 100 g−1 oven dry panel referred to 3.32 mg 100 g−1 oven dry panel for reference samples with 10 pbw filler) and improves water resistance, indicating better further dimensional stability and lower environmental impact. The study also shows that excessive filler content negatively affects strength parameters, confirming the importance of optimizing filler concentration. These results highlight the potential of hazelnut shells as an eco-friendly alternative filler in plywood production, contributing to waste valorization and environmental sustainability. This study supports the practical application of hazelnut shell fillers, promoting a circular economy and reducing reliance on traditional, less sustainable materials, thus providing a valuable solution for the wood composite industry.
SMPTC3: Secure Multi-Party Protocol Based Trusted Cross-Chain Contracts
Authors: Mao Hanyu , Nie Tiezheng , Yu Minghe , Dong Xiaomei , Li Xiaohua , Yu Ge
Published: August 2024

We propose an innovative approach called SMPTC3, designed specifically to enhance security and privacy in cross-chain transaction verification. This approach addresses multi-chain, multi-participant information exchange and large-scale cross-chain transfers, resisting various types of malicious attacks. We leverage the verifiability of cross-chain transactions based on smart contracts and innovatively transform transaction information into confidential sets, organizing them into quadratic secret polynomials. By utilizing secret sharing and random distribution techniques, we construct a secure multiparty computation method, tailored for cross-chain transactions. To enhance the efficiency of cross-chain transactions, we introduce cross-chain batch processing technology, grouping inter-chain transactions into cross-chain transaction sets. Unlike traditional distributed notary technologies, SMPTC3 designates honest participants as a cross-chain notary group, reducing the time required for redundant signature confirmations and significantly lowering the possibility of malicious notaries. Theoretical analysis and empirical experiments demonstrate that SMPTC3 is highly efficient in addressing cross-chain transaction security issues.
Towards Discriminability with Distribution Discrepancy Constrains for Multisource Domain Adaptation
Authors: Lu Yuwu , Huang Wanming
Published: August 2024

Multisource domain adaptation (MDA) is committed to mining and extracting data concerning target tasks from several source domains. Many recent studies have focused on extracting domain-invariant features to eliminate domain distribution differences. However, there are three aspects that require further consideration. (1) Efforts should be made to ensure the maximum correlation in the potential subspace between the source and target domains. (2) While aligning the marginal distribution, the conditional distribution must also be considered. (3) Merely aligning the source distribution and target distribution cannot guarantee sufficient differentiation for classification tasks. To address these problems, we propose a novel approach named towards discriminability with distribution discrepancy constrains for multisource domain adaptation (TD-DDC). Specifically, TD-DDC first mines features of maximal relations learned from all domains while constructing domain data distribution mean distance metrics for interdomain distribution adaptation. Simultaneously, we integrate discriminability into domain alignment, which means increasing the distance among labels that are distinct from one another while reducing the distance among labels that are the same. Our proposed method not only reduces the interdomain distributional differences but also takes into account the preservation of interdomain correlation and inter-category discrimination. Numerous experiments have shown that TD-DDC performs much better than its competitors on three visual benchmark test databases.
A Subspace-Based Frequency Synchronization Algorithm for Multicarrier Communication Systems
Authors: Wang Yung-Yi , Yang Shih-Jen
Published: August 2024

We present a subspace-based polynomial rooting algorithm to estimate the frequency bias (FB) of generalized frequency division multiplexing (GFDM) systems employing null subcarriers and repetitive sub-symbols. The estimation process is classified into fractional FB (FFB) and integer FB (IFB) estimation. The use of repetitive sub-symbols creates a quasi-periodic structure in the FB-distorted received signal, allowing the proposed algorithm to estimate the FFB using the root-MUSIC algorithm. Based on this, the proposed algorithm compensates for the FFB in the received signal and then estimates the null subcarrier pattern (NSP) in the frequency domain. As a result, the IFB estimate can be obtained in a maximum likelihood (ML) manner. Before the NSP estimation, this study uses a sub-symbol combiner to enhance signal strength of the FFB-aligned signal, ensuring the reliability of the IFB estimate. Computer simulations show that the proposed subspace-based algorithm has several advantages over traditional FB estimation methods: 1. Unlike some existing algorithms that use a training sequence to estimate FB, the proposed approach is a semi-blind algorithm because it can deliver information through repeated sub-symbols while estimating FB; 2. The proposed algorithm demonstrates excellent estimation accuracy compared to most traditional FB estimation algorithms; and 3. The proposed algorithm is computationally efficient, making it applicable to real-time applications in future communication systems.
Identification of Marine-Derived SLC7A11 Inhibitors: Molecular Docking, Structure-Based Virtual Screening, Cytotoxicity Prediction, and Molecular Dynamics Simulation
Authors: Chen Jiaqi , Li Xuan , Tao Jiahua , Luo Lianxiang
Published: August 2024

The search for anticancer drugs that target ferroptosis is a promising avenue of research. SLC7A11, a key protein involved in ferroptosis, has been identified as a potential target for drug development. Through screening efforts, novel inhibitors of SLC7A11 have been designed with the aim of promoting ferroptosis and ultimately eliminating cancer cells. We initially screened 563 small molecules using pharmacophore and 2D-QSAR models. Molecular docking and ADMET toxicity predictions, with Erastin as a positive control, identified the small molecules 42711 and 27363 as lead compounds with strong inhibitory activity against SLC7A11. Further optimization resulted in the development of a new inhibitor structure (42711_11). Molecular docking and ADMET re-screening demonstrated successful fragment substitution for this small molecule. Final molecular dynamics simulations also confirmed its stable interaction with the protein. These findings represent a significant step towards the development of new therapeutic strategies for ferroptosis-related diseases.
IL-1 Receptor Contributes to the Maintenance of the Intestinal Barrier via IL-22 during Obesity and Metabolic Syndrome in Experimental Model
Authors: Machado Melissa , Rodrigues Vanessa , Barbosa Sara , Elias-Oliveira Jefferson , Pereira Ítalo , Pereira Jéssica , Pacheco Thaílla , Carlos Daniela
Published: August 2024

Intestinal permeability and bacterial translocation are increased in obesity and metabolic syndrome (MS). ILC3 cells contribute to the integrity of intestinal epithelium by producing IL-22 via IL-1β and IL-23. This study investigates the role of IL-1R1 in inducing ILC3 cells and conferring protection during obesity and MS. For this purpose, C57BL/6 wild-type (WT) and IL-1R1-deficient mice were fed a standard diet (SD) or high-fat diet (HFD) for 16 weeks. Weight and blood glucose levels were monitored, and adipose tissue and blood samples were collected to evaluate obesity and metabolic parameters. The small intestine was collected to assess immunological and junction protein parameters through flow cytometry and RT-PCR, respectively. The intestinal permeability was analyzed using the FITC-dextran assay. The composition of the gut microbiota was also analyzed by qPCR. We found that IL-1R1 deficiency exacerbates MS in HFD-fed mice, increasing body fat and promoting glucose intolerance. A worsening of MS in IL-1R1-deficient mice was associated with a reduction in the ILC3 population in the small intestine. In addition, we found decreased IL-22 expression, increased intestinal permeability and bacterial translocation to the visceral adipose tissue of these mice compared to WT mice. Thus, the IL-1R1 receptor plays a critical role in controlling intestinal homeostasis and obesity-induced MS, possibly through the differentiation or activation of IL-22-secreting ILC3s.
Molecular Diversity of Ectomycorrhizal Fungi in Relation to the Diversity of Neighboring Plant Species
Authors: Zhang Weiwei , Xue Wenyan , Liu Jinliang , Zhu Hailan , Zhao Zhong
Published: August 2024

(1) Background: Plant diversity has long been assumed to predict soil microbial diversity. The mutualistic symbiosis between forest trees and ectomycorrhizal (EM) fungi favors strong correlations of EM fungal diversity with host density in terrestrial ecosystems. Nevertheless, in contrast with host tree effects, neighboring plant effects are less well studied. (2) Methods: In the study presented herein, we examined the α-diversity, community composition, and co-occurrence patterns of EM fungi in Quercus acutissima across different forest types (pure forests, mixed forests with Pinus tabuliformis, and mixed forests with other broadleaved species) to ascertain how the EM fungi of focal trees are related to their neighboring plants and to identify the underlying mechanisms that contribute to this relationship. (3) Results: The EM fungal community exhibited an overall modest but positive correlation with neighboring plant richness, with the associations being more pronounced in mixed forests. This neighboring effect was mediated by altered abiotic (i.e., SOC, TN, LC, and LP) and biotic (i.e., bacterial community) factors in rhizosphere soil. Further analysis revealed that Tomentella_badia, Tomentella_galzinii, and Sebacina_incrustans exhibited the most significant correlations with plant and EM fungal diversity. These keystone taxa featured low relative abundance and clear habitat preferences and shared similar physiological traits that promote nutrient uptake through contact, short-distance and medium-distance smooth contact-based exploration types, thereby enhancing the potential correlations between EM fungi and the neighboring plant community. (4) Conclusions: Our findings contribute to the comprehension of the effect of neighboring plants on the EM fungal community of focal trees of different forest communities and the biodiversity sensitivity to environmental change.