bluuebunny/crossref_metadata_2025 huggingface dataset:
Where does Russian Music Begin? The Case of Mikhail Glinka in the Biographical Series of “The Lives of Wonderful People”
Authors:   Petrozavodsk State A. K. Glazunov Conservatory, Kupets Lyubov A.
Published: April 2022

Since the second half of the 19th century, the figure of Mikhail Glinka has become the dominant one for constructing the total history of Russian music and understanding the significance of other Russian composers. Among the multitude of historical and theoretic research works pertaining to Russian musicology, especially noteworthy is the publication of the musician’s biography in the popular scholarly series “The Lives of Wonderful People” for the period of 1892– 2019. A long-term analysis of the author’s narratives in the historical and cultural context revealed the mechanisms of cultural recycling in the interpretation of Glinka’s personality and work: the phenomenon of “forgetting” and the effects of “double recycling” and the “personal approach.” Several models of biography are traced, the features of which resonate with the historical, cultural, and political guidelines of the era as well as with the professional preferences of the authors of these books. The book about Glinka written during the Silver Age of Russian culture presents a kind of a “life guide,” which peculiarly conflates the Victorian biographical canon and the psychological essay bearing a positivist accent. The Soviet image of the composer was being formed gradually, becoming fixed in the 1940s and 1950s. In its case, both the tendency to visualize narratives associated with the influence of the cinematograph (from the “novel about the composer” to the “movie plot for the young generation”) and the ideological partisanship in Glinka’s biography (the maximal approach to the canons of socialist realism) become characteristic feature. In the 21st century the leading principle in designing the image of the composer is the demythologization of the Soviet canon and the fundamental orientation towards the Internet public consisting primarily of the generation of millennials.
Synthesis of Cultural Forms as the Key Paradigm of the Postmodern and Metamodern Eras
Authors:   Far Eastern State Technical Fisheries University, Tsareva Nadezhda A. , Kuleshov Valery E. ,   Pacific Higher Naval College after S. O. Makarov
Published: April 2022

The postmodern era is being replaced by the metamodern era. A number of authors consider the question of the evolution of postmodernism as a continuation of the development of postmodernism in the new aesthetic paradigm of metamodern (Robin Akker, Alexander Pavlov, Hansi Freinacht). The necessity for reflecting on the state of culture in the 21st century has determined the relevance of this work. In order to understand the specificity of the metamodern culture, it is necessary to know its foundations; the idea of pluralization being one of them. Therefore, the subject of the analysis is the reflection on postmodern philosophers and their view on integration of knowledge and cultural forms. The purpose of the article is to examine the idea of the synthesis of cultural forms in the philosophy of postmodernism to understand the specificity of its implementation in metamodern culture. The turn to the conception of integration of knowledge and cultural forms in postmodernism provides deeper understanding of metamodern culture. The authors highlight the following features of the synthesis of cultural forms in the metamodern era: digitalization of the cultural space; the synergy of art and everyday life; the trend for the pluralism of values. The contours of development of pluralistic paradigm in 21st century culture is identified. It is noted that in the present day we may observe the process of integration of various forms of culture in different spheres of social life. Pluralism becomes the key paradigm of the metamodern era. Consequently, the postmodern idea of synthesis is actualized in the metamodern culture, i.e., a development of postmodern culture processes takes.
Infrastructuring ageing: Theorizing non-human agency in ageing and technology studies
Authors: Ertner Sara Marie
Published: April 2022

Scholars of ageing and technology are becoming increasingly interested in how technology and ageing can be seen as mutually constitutive, an in­terest that is beginning to form new research agendas, alliances and fields of their own. Different concepts have been used to theorise and analyse this relationship of mutual construction. This article explores a concept from Science and technology studies, which has not previously been put in direct relation to ageing, namely the concept of infrastructure. It pro­poses the notion of “infrastructuring ageing” as a theoretical-analytical approach for studying the mutual constitution of ageing and technol­ogy. This approach implies slightly new versions of, or attentions to, the non-human actor, agency and socio-technical transformation, and opens up to fresh ethnographic views on the social, material and techno-political transformations of ageing.
Transcriptome-Wide Dynamics of m6A Methylation in Tumor Livers Induced by ALV-J Infection in Chickens
Authors: Zhao Qiqi , Yao Ziqi , Chen Liyi , He Yaai , Xie Zi , Zhang Huanmin , Lin Wencheng , Chen Feng , Xie Qingmei , Zhang Xinheng
Published: April 2022

Avian Leukosis Virus Subgroup J (ALV-J) is a tumorigenic virus with high morbidity and rapid transmission. N6-methyladenosine (m6A) is a common epigenetic modification that may be closely related to the pathogenicity of ALV-J. Currently, there are no reports on whether m6A modification is related to ALV-J induced tumor formation. In this study, we used methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) to examine the differences in m6A methylation and gene expression in normal livers and ALV-J-induced tumor livers systematically, with functional enrichment and co-expression analysis. The results identified 6,541 m6A methylated peaks, mainly enriched in CDS, and more than 83% of the transcripts contained 1-2 m6A peaks. For RNA-seq, 1,896 and 1,757 differentially expressed mRNAs and lncRNAs were identified, respectively. Gene enrichment analysis indicated that they may be involved in biological processes and pathways such as immunology-related and apoptosis. Moreover, we identified 17 lncRNAs, commonly existing in differently expressed methylome and transcriptome. Through co-expression analysis, 126 differentially expressed lncRNAs, and 18 potentially m6A-related methyltransferases were finally identified and connected, suggesting that m6A modifications might affect gene expression of lncRNAs and play a role in ALV-J induced tumor formation. This study provides the first comprehensive description of the m6A expression profile in tumor livers induced by ALV-J infection in chickens, which provides a basis for studying the role of m6A modification in ALV-J induced tumorigenesis. This study provides clues for studying the epigenetic etiology and pathogenesis of ALV-J.
Interleukin-34 Mediates Cross-Talk Between Stromal Cells and Immune Cells in the Gut
Authors: Monteleone Giovanni , Franzè Eleonora , Troncone Edoardo , Maresca Claudia , Marafini Irene
Published: April 2022

Initially known as a cytokine produced by and regulating the function of monocytes and macrophages, interleukin-34 (IL-34) can be synthesized by many cell types and interacts with receptors expressed by multiple immune and non-immune cells. IL-34 is constitutively expressed in the healthy human small intestine and colon and its production is markedly increased in damaged gut of patients with Crohn’s disease and patients with ulcerative colitis, the main forms of chronic inflammatory bowel diseases (IBD) in human beings. Circumstantial evidence suggests that, in these pathologies, IL-34 plays a crucial role in mediating cross-talk between immune cells and stromal cells, thereby promoting activation of signalling pathways, which amplify the ongoing mucosal inflammation as well as production of fibrogenic molecules. In this article, we summarize the available data supporting the multiple effects of IL-34 in human IBD with particular attention to the role of the cytokine in immune and stromal cell interactions.
Proteomic and Antibody Profiles Reveal Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19
Authors: He Chuan-Yu , Yang Jiang-Hua , Ye Yin-Bo , Zhao Hai-Long , Liu Meng-Zhi , Yang Qi-Lin , Liu Bao-Shan , He Sun , Chen Ze-Liang
Published: April 2022

Brucellosis is an important zoonotic disease that causes great economic losses. Vaccine immunisation is the main strategy for the prevention and control of brucellosis. Although live attenuated vaccines play important roles in the prevention of this disease, they also have several limitations, such as residual virulence and difficulty in the differentiation of immunisation and infection. We developed and evaluated a new bacterial ghost vaccine of Brucella abortus A19 by a new double inactivation method. The results showed that the bacterial ghost vaccine of Brucella represents a more safe and efficient vaccine for brucellosis. We further characterised the antigenic components and signatures of the vaccine candidate A19BG. Here, we utilised a mass spectrometry-based label-free relative quantitative proteomics approach to investigate the global proteomics changes in A19BGs compared to its parental A19. The proteomic analysis identified 2014 proteins, 1116 of which were differentially expressed compared with those in A19. The common immunological proteins of OMPs (Bcsp31, Omp25, Omp10, Omp19, Omp28, and Omp2a), HSPs (DnaK, GroS, and GroL), and SodC were enriched in the proteome of A19BG. By protein micro array-based antibody profiling, significant differences were observed between A19BG and A19 immune response, and a number of signature immunogenic proteins were identified. Two of these proteins, the BMEII0032 and BMEI0892 proteins were significantly different (P < 0.01) in distinguishing between A19 and A19BG immune sera and were identified as differential diagnostic antigens for the A19BG vaccine candidate. In conclusion, using comparative proteomics and antibody profiling, protein components and signature antigens were identified for the ghost vaccine candidate A19BG, which are valuable for further developing the vaccine and its monitoring assays.
Fragile X Mental Retardation Protein Mediates the Effects of Androgen on Hippocampal PSD95 Expression and Dendritic Spines Density/Morphology and Autism-Like Behaviors Through miR-125a
Authors: Chen Huan , Qiao Dan , Wang Chang , Zhang Bohan , Wang Zhao , Tang Longmei , Wang Yibo , Zhang Ran , Zhang Yizhou , Song Leigang , Zuo Hongchun , Guo Fangzhen , Wang Xia , Li Sha , Cui Huixian
Published: April 2022

Dysregulated synaptic plasticity is a key feature of neurodevelopmental disorders, including autism. This study investigated whether Fragile X mental retardation protein (FMRP), a selective RNA-binding protein that regulates synaptic protein expression by interacting with miRNAs, mediates the effects of androgens that play an important role in regulating the synaptic plasticity in the hippocampus. Experiments using mouse hippocampal neuron HT22 cells demonstrated that dihydrotestosterone (DHT) increased the expression of postsynaptic density protein 95 (PSD95) by inhibiting FMRP expression. Administration of miR-125a inhibitor upregulated the PSD95 expression and significantly increased the DHT-induced upregulation of PSD95. FMRP knockdown in HT22 cells reduced the expression of miR-125a. Moreover, miR-125a inhibitor upregulated the PSD95 expression in the DHT-treated HT22 cells with FMRP knockdown. Subsequently, the effects of androgen-mediated via FMRP in regulating neural behaviors and PSD95 expression and dendritic spines density/morphology were investigated using Fmr1 knockout (KO) and wild-type littermate (WT) mice. The castration of WT mice reduced the androgen levels, aggravated anxiety and depression, and impaired learning and memory and sociability of mice. DHT supplementation post-castration reversed the alterations in density and maturity of dendritic spines of hippocampal neurons and behavioral disorders in WT mice; however, it did not reveal such effects in Fmr1 KO mice. Further, immunohistochemical staining and western blotting analyses after knocking down miR-125a revealed similar effects of castration and post-castration DHT supplementation on PSD95 protein expression. These findings clarified that FMRP mediated the effects of DHT through miR-125a in regulating the expression of hippocampal synaptic protein PSD95. This study provides evidence for the neuroprotective mechanism of androgen in PSD95 expression and dendritic spines density/morphology and suggests that treatment interventions with androgen could be helpful for the management of synaptic plasticity disorders.
Impact of Treatment Modalities on Prognosis in Patients With Renal Collecting Duct Carcinoma: A Population-Based Study
Authors: Qian Xiaoyuan , Xu Jinzhou , Liu Chenqian , Zhong Mingliang , Hong Senyuan , Qian Can , Zhu Jianning , Zhang Jiaqiao , Wang Shaogang
Published: April 2022

Objective

Renal collecting duct carcinoma (CDC) is an extremely rare disease with few studies, and the current understanding of its prognosis is limited. We used the Surveillance, Epidemiology, and End Results (SEER) registry data to explore the prognostic factors and effect of treatment modalities on the overall survival (OS) and cancer-specific survival (CSS) in patients with CDC.

Methods

Patients’ information of CDCs diagnosed by pathological examination between 2000 and 2018 was extracted from the SEER database. The Kaplan–Meier method was used to calculate OS and CSS and log-rank tests to evaluate the differences in OS and CSS. The associations between clinicopathological variables and survival outcomes were assessed with the Cox proportional hazard model. A directed acyclic graph (DAG) was drawn to recognize confounding factors and to obtain the multivariable regression model, and the impact of surgery, radiotherapy, and chemotherapy on OS and CSS was analyzed, respectively.

Results

A total of 242 patients with CDC were enrolled. The median OS and CSS time were 17 and 21 months, respectively. The OS rates at 1, 2, and 5 years were 56.9%, 41.9%, and 30.0%, respectively, while the CSS rates at 1, 2, and 5 years were 60.1%, 47.5%, and 34.8%, respectively. Patients who had a large tumor size, poor pathological grade, and advanced TNM classification exhibited worse survival outcomes. Univariable and multivariable Cox regression analyses revealed that surgery, chemotherapy, T stage, N stage, and M stage were independent prognostic factors for OS and CSS. The DAG-guided multivariate Cox regression model revealed that surgery and chemotherapy improved OS and CSS.

Conclusions

CDC is an exceedingly rare disease and has malignant behavior. Most patients have a high pathological grade and advanced TNM stage at diagnosis and exhibited poor survival. Resection of all visible tumors including metastatic lesions or chemotherapy can be beneficial to prognosis, while healthier benefits are less likely to receive radiotherapy. More relevant studies with larger samples are needed to verify the value of surgery and adjuvant therapy in the treatment of CDCs.
Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients
Authors: Guarda Vittoria , Schroeder Lea , Pawlita Michael , Ikenberg Kristian , Rupp Niels J. , Jochum Wolfram , Stoeckli Sandro J. , Holzinger Dana , Broglie Martina A.
Published: April 2022

Objectives

The natural history of HPV-related oropharyngeal squamous cell carcinoma (OPSCC) is still largely unknown. Since reports of second primary tumors (SPTs) in patients with HPV-related OPSCCs are increasing, a multifocal HPV infection, hinting a «virus-induced field effect», has been hypothesized. This study aimed to investigate the HPV-prevalence in normal appearing oropharyngeal tissue in patients with OPSCCs.

Materials and Methods

49 OPSCC patients undergoing panendoscopy were prospectively enrolled. Tumor specimens and biopsies of normal appearing oropharyngeal tissue adjacent to and distant from the index OPSCC underwent histopathological examination, p16INK4A immunohistochemical staining, HPV DNA and mRNA-detection. Patient characteristics and follow-up data on SPTs were obtained.

Results

26 of 49 (53%) OPSCC were positive for HPV DNA and p16INK4A. HPV mRNA was detected in 23 of 26 (88%) of these tumor samples. HPV DNA was detected in 36% adjacent mucosa and in 17% distant mucosa samples and only in patients with an HPV-related index OPSCC. HPV mRNA could not be detected in tumor-free distant and adjacent mucosa samples. No evidence of association between HPV detection in normal appearing mucosa and development of second primary tumors was found.

Conclusions

HPV was detectable but not transcriptionally active in adjacent/distant tumor-free oropharyngeal tissue. This suggests that a multifocal HPV infection, hinting a «virus-induced fielcd cancerization», may not be pertaining to HPV-related OPSCC.
Markers of Hypoxia and Metabolism Correlate With Cell Differentiation in Retina and Lens Development
Authors: Gardiner Tom A. , Branagh Tiarnan , Tipping Nuala , McDonald Denise M.
Published: April 2022

Recent studies have provided novel insights of co-development of the neural and vascular elements of the retina. Knowledge of these relationships are crucial to understand the impact of therapeutic measures in Retinopathy of Prematurity (ROP). ROP is imposed by therapeutic oxygen upon immature retinal blood vessels and neural cells causing delayed development and vascular regression. However, the impact of hyperoxia on developing retinal neurons is less understood because some aspects of normal development remain unknown. The metabolic changes during differentiation of retinal progenitor cells to functional neurons is one such aspect. We correlated immunomarkers of hypoxia with markers of metabolic change in developing retinal neurons during the early postnatal period in mice. The same marker proteins were studied in secondary lens fiber differentiation at postnatal day-3 (P3). Nuclear localization of the oxygen-sensitive subunits of hypoxia inducible factor, HIF-1α and HIF-2α was correlated with increasing mitochondrial content in differentiating neurons. Nuclear HIF was also correlated with AMP-dependent protein kinase (AMPK), and the AMPK phosphorylation target PPAR-gamma coactivator-1alpha (PGC-1α), the principal regulator of mitochondrial biogenesis. Expression of AMPK, PGC1α and HIF-2α in secondary fiber differentiation was visible in each profile of the lens equator. Strong nuclear localization for all markers was present at the onset of secondary fiber differentiation, and reflected changes in size, mitochondrial content, and metabolism. We speculate that the ‘physiological hypoxia’ that drives retinal vascular development is cell-specific and reliant upon neuronal differentiation and mitochondrial biogenesis. We suggest that the onset of differentiation increases energy consumption that is detected by AMPK. In turn AMPK increases mitochondrial biogenesis via PGC-1α. Mitochondrial oxygen consumption may then create intracellular hypoxia that activates HIF. This progression is congruent with the expression of these markers in secondary lens fiber differentiation and nuclear localization of HIF-2α. Nuclear localization of HIF-1α and HIF-2α in the postnatal retina is less defined than in the lens as it may involve the remnant of HIF expression from the embryonic period that is sustained and increased by intracellular hypoxia caused by increasing mitochondrial oxygen consumption. This the first report of the involvement of HIF-2α, AMPK and PGC-1α in lens development.
Mapping of the Quantitative Trait Loci and Candidate Genes Associated With Iron Efficiency in Maize
Authors: Xu Jianqin , Qin Xiaoxin , Zhu Huaqing , Chen Fanjun , Fu Xiuyi , Yu Futong
Published: April 2022

Iron (Fe) is a mineral micronutrient for plants, and Fe deficiency is a major abiotic stress in crop production because of its low solubility under aerobic and alkaline conditions. In this study, 18 maize inbred lines were used to preliminarily illustrate the physiological mechanism underlying Fe deficiency tolerance. Then biparental linkage analysis was performed to identify the quantitative trait loci (QTLs) and candidate genes associated with Fe deficiency tolerance using the recombinant inbred line (RIL) population derived from the most Fe-efficient (Ye478) and Fe-inefficient (Wu312) inbred lines. A total of 24 QTLs was identified under different Fe nutritional status in the Ye478 × Wu312 RIL population, explaining 6.1–26.6% of phenotypic variation, and ten candidate genes were identified. Plants have evolved two distinct mechanisms to solubilize and transport Fe to acclimate to Fe deficiency, including reduction-based strategy (strategy I) and chelation-based strategy (strategy II), and maize uses strategy II. However, not only genes involved in Fe homeostasis verified in strategy II plants (strategy II genes), which included ZmYS1, ZmYS3, and ZmTOM2, but also several genes associated with Fe homeostasis in strategy I plants (strategy I genes) were identified, including ZmFIT, ZmPYE, ZmILR3, ZmBTS, and ZmEIN2. Furthermore, strategy II gene ZmYS1 and strategy I gene ZmBTS were significantly upregulated in the Fe-deficient roots and shoots of maize inbred lines, and responded to Fe deficiency more in shoots than in roots. Under Fe deficiency, greater upregulations of ZmYS1 and ZmBTS were observed in Fe-efficient parent Ye478, not in Fe-inefficient parent Wu312. Beyond that, ZmEIN2 and ZmILR3, were found to be Fe deficiency-inducible in the shoots. These findings indicate that these candidate genes may be associated with Fe deficiency tolerance in maize. This study demonstrates the use of natural variation to identify important Fe deficiency-regulated genes and provides further insights for understanding the response to Fe deficiency stress in maize.
Proving Fixed-Point Theorems Employing Fuzzy (σ,𝒵)-Contractive-Type Mappings
Authors: Saleh Hayel N. , Imdad Mohammad , Sessa Salvatore , Di Martino Ferdinando
Published: April 2022

In this article, the concept of fuzzy (σ,Z)-contractive mappings is introduced in the setting of fuzzy metric spaces. Thereafter, we utilize our newly introduced concept to prove some existence and uniqueness theorems in M-complete fuzzy metric spaces. Our obtained theorems extend and generalize the corresponding results in the existing literature. Moreover, some examples are adopted to exhibit the utility of the newly obtained results.
Comparison of Fluorescent Techniques Using Two Enzymes Catalysed for Measurement of Atmospheric Peroxides
Authors: Sun Jieya , Ning Yi , Chen Xuan , Zhang Xinlu , Ren Yanjun , Li Bin
Published: April 2022

Atmospheric peroxides, especially hydrogen peroxide (H2O2), are essential oxidants. The peroxide concentration is closely related to the extent of OH radicals and the O3 cycle in the tropospheric atmospheric chemistry. However, only a few studies have investigated their atmospheric concentrations in China because of inadequacies in the measurement techniques or higher costs of analytical instruments. Therefore, it is essential to design a suitable analysis method of peroxides with higher sensitivity, lower detection limit, and low cost. In view of that, this study investigated the optimum analysis conditions of two H2O2 analytical techniques: the high-performance liquid chromatography (HPLC) with fluorescence detection using two-enzyme catalysis of horseradishperoxidase (HRP method) and Hemin (Hemin method). Furthermore, these two analysis methods were systematically compared in terms of detection limit, calibration curve, precision, accuracy, and applicability for the first time. The findings showed that the HRP method had a lower detection limit, higher sensitivity, and better applicability for detecting H2O2 and methyl hydroperoxide (MHP) than the Hemin method. Moreover, the HRP method is better suitable for H2O2 and MHP detection, which requires low detection limits and high sensitivity. Besides this, the Hemin method is inexpensive and is more suitable for detecting hydroxyl alkyl peroxides (C ≥ 3). The atmospheric concentrations (average) of H2O2 and MHP were 0.60 ± 0.37 ppb and 0.081 ± 0.039 ppb, respectively, as determined by the HRP method. Importantly, atmospheric peroxide concentrations were higher on sunny days than on cloudy days in Beijing in September 2016. H2O2 concentrations showed a diurnal variation with the lowest value in the morning and two peaks at 13:00–17:00. In contrast, MHP concentrations were lowest in the morning and highest after 17:00. Photochemical reactions were responsible for the production of H2O2 and MHP. The reactions of O3 and olefins emitted by motor vehicles also caused H2O2 concentration to increase during the evening rush hour.
Stochastic Dynamic Aircraft System Conflict Distribution under Uncertainties
Authors: Dudoit Anrieta , Rimša Vytautas , Bogdevičius Marijonas
Published: April 2022

A dynamic aircraft system conflict (concurrent event) situation exists when a time with a loss (-es) of separation (LOS) in their true or predicted trajectories is determined. Regional air traffic management (ATM) programs aim to make ATM safer and more efficient through a higher level of automation for such processes as dynamic aircraft systems concurrent events detection and, consequently, resolution. Therefore, wind and aircraft speed uncertainty parameters should be properly addressed. This paper offers an approach to a dynamic aircraft system flying under a certain concurrent event situation and demonstrates situation stochastic distribution results (output) based on determined wind speed values (while wind direction angles and the dynamic aircraft system speed values are random). Based on these facts, the stochastic dynamic aircraft system conflict distribution information under determined and random parameters might be retrieved at any specific (preferred) time moment. The observations of this study disclosed that such stochastic output data might have a certain impact on safety matters (potential “domino effect” conflicts on a horizontal plane) and on the efficiency (i.e., flight distance which eventually is a determinant of flight time, fuel costs, delays, emissions, monitoring, etc.).
The Application of Radiochemical Measurements of PWR Spent Fuel for the Validation of Burnup Codes
Authors: Oettingen Mikołaj
Published: April 2022

The paper shows the usage of destructive assay data from PWR fuel samples for the validation of the NFCSS burnup system developed by the IAEA. The results of radiochemical measurements of spent fuel isotopic composition were compared with the results of numerical modelling. In total, 254 samples from 15 PWRs, incorporated in the SFCOMPO database, were applied in the validation study. The paper shows the trends observed in the calculated-to-experimental ratios for eight major actinides and six minor actinides present in spent nuclear fuel. The data were quantified and analysed considering the enrichment, burnup and origin. The obtained results were compared with other studies on burnup validation using different numerical tools. In general, the results of numerical modelling for major actinides show rather good consistency with radiochemical measurements, while the results for minor actinides are less accurate.
Fouling Behavior and Dispersion Stability of Nanoparticle-Based Refrigeration Fluid
Authors: Ponticorvo Eleonora , Iuliano Mariagrazia , Cirillo Claudia , Maiorino Angelo , Aprea Ciro , Sarno Maria
Published: April 2022

Nanofluids as heat transfer fluids have been acquiring popularity ever since their beginning. Therefore, the refrigeration research could not keep itself away from the ever-rising horizon of nanofluid applications. On the other hand, nanofluid stability remains the critical bottleneck for use. A significant reduction in nanofluids’ performance can derivate from instability phenomena. Looking to industrial applications, nanofluid long-term stability and reusability are crucial requisites. Nanoparticles’ deposits induce microchannel circuit obstruction, limiting the proper functioning of the device and negating the beneficial characteristics of the nanofluid. The aggregation and sedimentation of the particles may also determine the increased viscosity and pumping cost, and reduced thermal properties. So, there is a need to address the features of nanofluid starting from realization, evaluation, stabilization methods, and operational aspects. In this review, investigations of nanorefrigerants are summarized. In particular, a description of the preparation procedures of nanofluids was reported, followed by a deep elucidation of the mechanism of nanofluid destabilization and sedimentation, and finally, the literature results in this field were reviewed.
Experimental Research on the Dynamic Characteristics and Voltage Uniformity of a PEMFC Stack under Subzero Temperatures
Authors: Liu Pengcheng , Xu Sichuan
Published: April 2022

The working life of a stack is basically determined by the behavior of its weakest monomer; therefore, during a dynamic loading process, high-voltage uniformity plays a key role in stack durability. This work experimentally investigated dynamic responses and voltage uniformity with different loading strategies from an initial state (open-circuit state) to a target current at subzero temperatures. The results presented that the maximum voltage coefficient variation Cv values, representing the relative standard deviation of the monomer voltage, increased from 19.75 to 35.65 and the voltage uniformity sharply decreased with the increase in loading sizes at the stack temperature of −4 °C. It was possible that this could result in the instability of the single cell voltage output and a voltage uniformity fluctuation with a high step loading amplitude. The voltage uniformity became worse as the stack temperature decreased from −2 °C to −8 °C, the voltage uniformity between single cells of the stack continued to deteriorate under a step current density of 0.15 A/cm2 at a relatively low temperature of −8 °C. By comparing several constant loading rate strategies, it could be ascertained that reducing the loading rate was conducive to voltage uniformity in the whole investigated process. A comparison of the different loading rates indicated that the square increasing loading rate strategy is the best strategy among them and a Cv threshold of 5.5 can be obtained during the whole load process at a temperature of −4 °C, which might be a guideline and provide a reference in actual engineering applications for PEMFC dynamic loading responses.
Mission Himalaya: Exploring the Impact of a Supported High-Altitude Mountaineering Expedition on the Well-Being and Personal Development of UK Military Veterans
Authors: Kay Christopher William Philip , Wingfield Harriet Laura , McKenna Jim
Published: April 2022

Meaningful, positive, emotional and challenging adventurous activities may generate personal growth or recovery from ill health or injury. In this study, we used a distinctive longitudinal and immersive research approach to explore the psychological impact of a high-altitude expedition to the Nepalese Himalaya on 10 (9 males) UK military veterans with longstanding well-being concerns. In the 12 months prior to the expedition, participants took part in three training weekends in the UK mountains. During the expedition, instructors—who were all experienced health coaches—facilitated reflective practices with the beneficiaries throughout, focusing on experiential transfer to day-to-day lives after the expedition. Follow-up interviews, conducted up to 18-months post-expedition, identified that the most desirable changes aligned with the three innate psychological needs of self-determination theory: autonomy, competence and relatedness. The routines established during the preparation stage and during the expedition itself activated a renewed energy for personal improvement. At 18 months post-expedition, the key changes reflected altered perspective, employment skills and work–life balance, increased physical activity and enhanced personal awareness and mindfulness. Importantly, supported by regular health coaching and focused on the transfer of learning, expeditions can activate meaningful long-term changes to the well-being and personal development of military veterans.
Impact of Short-Term Air Pollution on Respiratory Infections: A Time-Series Analysis of COVID-19 Cases in California during the 2020 Wildfire Season
Authors: Ademu Lilian Ouja , Gao Jingjing , Thompson Onah Peter , Ademu Lawrence Anebi
Published: April 2022

The 2020 California wildfire season coincided with the peak of the COVID-19 pandemic affecting many counties in California, with impacts on air quality. We quantitatively analyzed the short-term effect of air pollution on COVID-19 transmission using county-level data collected during the 2020 wildfire season. Using time-series methodology, we assessed the relationship between short-term exposure to particulate matter (PM2.5), carbon monoxide (CO), nitrogen dioxide (NO2), and Air Quality Index (AQI) on confirmed cases of COVID-19 across 20 counties impacted by wildfires. Our findings indicate that PM2.5, CO, and AQI are positively associated with confirmed COVID-19 cases. This suggests that increased air pollution could worsen the situation of a health crisis such as the COVID-19 pandemic. Health policymakers should make tailored policies to cope with situations that may increase the level of air pollution, especially during a wildfire season.
Physical and Motor Fitness Tests for Older Adults Living in Nursing Homes: A Systematic Review
Authors: Galhardas Luis , Raimundo Armando , Del Pozo-Cruz Jesús , Marmeleira José
Published: April 2022

This systematic review aimed to identify the physical/motor fitness tests for nursing home residents and to examine their psychometric properties. Electronic databases were searched for articles published between January 2005 and October 2021 using MeSh terms and relevant keywords. Of the total of 4196 studies identified, 3914 were excluded based on title, abstracts, or because they were duplicates. The remaining 282 studies were full-text analyzed, and 41 were excluded, resulting in 241 studies included in the review. The most common physical component assessed was muscle strength; 174 (72.2%) studies assessed this component. Balance (138 studies, 57.3%) and agility (102 studies, 42.3%) were the second and third components, respectively, most widely assessed. In this review, we also describe the most used assessment tests for each physical/motor component. Some potentially relevant components such as manual dexterity and proprioception have been little considered. There are few studies assessing the psychometric properties of the tests for nursing home residents, although the data show that, in general, they are reliable. This review provides valuable information to researchers and health-care professionals regarding the physical/motor tests used in nursing home residences, helping them select the screening tools that could most closely fit their study objectives.
Novel Physical Fitness Fuzzy Evaluation Model for Individual Health Promotion
Authors: Chen Kuen-Suan , Hsieh Tzung-Hua
Published: April 2022

Physical fitness level plays a significant role in health promotion. Cardiorespiratory endurance, muscular endurance, muscle power, and flexibility are the four key indicators of physical fitness level, listed as one of the important fields of preventive medicine. Some studies targeted at students, based on statistical inference, have put forward a set of physical fitness evaluation methods to see whether they have reached the level of healthy physical fitness. Testing and monitoring of individual physical fitness takes up little time and requires a small sample dataset; this paper hence proposed an evaluation and analysis model that suits individual physical fitness by means of a fuzzy evaluation method suitable for evaluating small sample datasets. This paper developed the evaluation model based on the upper confidence limit of the physical fitness evaluation index so that it could reduce the risk of misjudgment caused by sampling error. At the same time, a simple and easy-to-use fuzzy evaluation form was developed as an evaluation interface, which can present the whole picture of all evaluation indicators as well as have good and convenient management performance. Accordingly, it can help every individual simultaneously monitor multiple physical fitness indicators to ensure that each physical fitness index can meet the requirement of healthy physical fitness.
Body-Esteem, Self-Esteem and Loneliness among Social Media Young Users
Authors: Pop Lavinia Maria , Iorga Magdalena , Iurcov Raluca
Published: April 2022

The use of social networking sites for socializing, having fun, solving academic tasks or even getting counselling for health-related problems is now inevitable. Methods: A total of 427 medical students, who are users of social media sites, were included in the research. Data about socio-demographic, anthropometric, and self-rated items regarding satisfaction with physical and mental health were collected. Three psychological tools were also used to measure self-esteem (Rosenberg Self-Esteem Scale), body-esteem (Body Esteem Scale for Adolescents and Adults) and loneliness (UCLA Loneliness Scale). Collected data were analyzed using SPSS version 23. Results: Students use these networks for socialization (49.0%), entertainment (31.1%) and academic tasks (19.9%), spending 3.38 ± 0.80 h per day on SNSs. Less than half of them (47.5%) compared themselves to other SNS profiles. The use of Snapchat was found to be strongly positively correlated with self-esteem, and weight status was negatively correlated with the use of TikTok. More than three-quarters declared that they exercised to lose weight or to prevent weight gain. Participants were found to have a high level of body esteem. Almost half of the students proved to have a moderate to a high level of loneliness. Age and gender were found to be important: the younger the user, the higher the scores for loneliness and feeling depressed, and the greater the number of hours on SNSs. The total score for self-esteem was significantly higher in men than in women, and male students appreciated themselves as being in a better state of mental health than women. Conclusions: The results prove a relationship between the use of SNSs and the presence of loneliness, self-esteem and body-esteem, with gender differences. However, the use of SNSs should not be neglected in clinical settings, and are a good means of reaching patients and providing medical and psychological intervention.
Needs to Create Healthy Living Environments—A Two-Stage Delphi Survey in Europe to Identify Facilitating Factors and Barriers in Municipal Health Promotion
Authors: Quilling Eike , Kuchler Maja , Tollmann Patricia , Osterhoff Anke , Leimann Janna
Published: April 2022

(1) In the field of health promotion, municipalities offer opportunities to reduce SES-based health inequalities by addressing vulnerable communities. This research project aims to identify facilitating and inhibiting factors for the creation of healthy living environments. (2) After preliminary literature and qualitative research work, an online-based Delphi survey was conducted (December 2020–March 2021). This included the rating and commentating of 22 theses at two times, whereby the results of the first round of rating were visible to the participants the second time. (3) Twelve experts from seven European countries participated in the Delphi survey across both rounds (1st round: n = 37; 12 countries). The consensus was particularly clear with regard to providing resources, which, in turn, are especially necessary for involving target groups in health promotion. (4) The results illustrate the relevance of further cross-national exchange. Certain aspects however, such as the HiAP approach or strategies to reach disadvantaged groups, are still challenging in practice. In order to develop concrete recommendations, the theses need to be further operationalised. The Delphi method offers a suitable possibility to map international expertise in this field and with a focus on health equity.
Infant Young Child Feeding Practices in an Indian Maternal–Child Birth Cohort in Belagavi, Karnataka
Authors: Khandelwal Shweta , Kondal Dimple , Chakravarti Anindita Ray , Dutta Soumam , Banerjee Bipsa , Chaudhry Monica , Patil Kamal , Swamy Mallaiah Kenchaveeraiah , Ramakrishnan Usha , Prabhakaran Dorairaj , Tandon Nikhil , Stein Aryeh D.
Published: April 2022

Poor infant young child feeding (IYCF) practices result in malnutrition, poor psychosocial development, poor school performance and less productivity in later life, thereby perpetuating a vicious cycle. The current study aims to characterize the IYCF practices during the first year of life in a maternal–child birth cohort (DHANI) in Belagavi, Karnataka, India. We collected data from the dyad at birth, 6 and 12 months postpartum. We examined dietary diversity among these infants at 12 months using WHO criteria. A total of 902 live births were recorded, and 878 mother–child pairs completed the 12-month follow up. The overall prevalence of early (within 1 h of delivery) initiation of breastfeeding (EIBF) was 77.9%, and that of exclusive breastfeeding (EBF) at 6 months was 52.4%. At 12 months, most (90%) infants were breastfed, while 39% also received formula. The large majority (94.4%) of infants met minimum meal frequency (MMF), but only 55% of infants were receiving a minimum acceptable diet (MAD). The mean dietary diversity (DD) score was 4.7 ± 1.1. Only 21.9% of infants consumed egg and/or flesh food. A large proportion (33.8%) of infants received no vegetables and/or fruits till 12 months of age. Consumption of sweet beverage was 4.8%, but consumption of ultra-processed foods high in trans-fats, sugars and salt was high (85.8%). High-quality, sustainable and scalable interventions to enhance knowledge and support positive behaviour change for adopting and implementing better IYCF practices may be urgently needed in low- and middle-income group settings to improve diet diversity and overall nutritional intake amongst young children.
Forecasting Multiple Groundwater Time Series with Local and Global Deep Learning Networks
Authors: Clark Stephanie R. , Pagendam Dan , Ryan Louise
Published: April 2022

Time series data from environmental monitoring stations are often analysed with machine learning methods on an individual basis, however recent advances in the machine learning field point to the advantages of incorporating multiple related time series from the same monitoring network within a ‘global’ model. This approach provides the opportunity for larger training data sets, allows information to be shared across the network, leading to greater generalisability, and can overcome issues encountered in the individual time series, such as small datasets or missing data. We present a case study involving the analysis of 165 time series from groundwater monitoring wells in the Namoi region of Australia. Analyses of the multiple time series using a variety of different aggregations are compared and contrasted (with single time series, subsets, and all of the time series together), using variations of the multilayer perceptron (MLP), self-organizing map (SOM), long short-term memory (LSTM), and a recently developed LSTM extension (DeepAR) that incorporates autoregressive terms and handles multiple time series. The benefits, in terms of prediction performance, of these various approaches are investigated, and challenges such as differing measurement frequencies and variations in temporal patterns between the time series are discussed. We conclude with some discussion regarding recommendations and opportunities associated with using networks of environmental data to help inform future resource-related decision making.
JAK2 Inhibitor, Fedratinib, Inhibits P-gp Activity and Co-Treatment Induces Cytotoxicity in Antimitotic Drug-Treated P-gp Overexpressing Resistant KBV20C Cancer Cells
Authors: Oh Yunmoon , Lee Jin-Sol , Lee Ji Sun , Park Jae Hyeon , Kim Hyung Sik , Yoon Sungpil
Published: April 2022

P-glycoprotein (P-gp) overexpression is one of the major mechanisms of multidrug resistance (MDR). Previously, co-treatment with Janus kinase 2 (JAK2) inhibitors sensitized P-gp-overexpressing drug-resistant cancer cells. In this study, we assessed the cytotoxic effects of JAK2 inhibitor, fedratinib, on drug-resistant KBV20C cancer cells. We found that co-treatment with fedratinib at low doses induced cytotoxicity in KBV20C cells treated with vincristine (VIC). However, fedratinib-induced cytotoxicity was little effect on VIC-treated sensitive KB parent cells, suggesting that these effects are specific to resistant cancer cells. Fluorescence-activated cell sorting (FACS), Western blotting, and annexin V analyses were used to further investigate fedratinib’s mechanism of action in VIC-treated KBV20C cells. We found that fedratinib reduced cell viability, increased G2 arrest, and upregulated apoptosis when used as a co-treatment with VIC. G2 phase arrest and apoptosis in VIC–fedratinib-co-treated cells resulted from the upregulation of p21 and the DNA damaging marker pH2AX. Compared with dimethyl sulfoxide (DMSO)-treated cells, fedratinib-treated KBV20C cells showed two-fold higher P-gp-inhibitory activity, indicating that VIC–fedratinib sensitization is dependent on the activity of fedratinib. Similar to VIC, fedratinib co-treatment with other antimitotic drugs (i.e., eribulin, vinorelbine, and vinblastine) showed increased cytotoxicity in KBV20C cells. Furthermore, VIC–fedratinib had similar cytotoxic effects to co-treatment with other JAK2 inhibitors (i.e., VIC–CEP-33779 or VIC–NVP-BSK805) at the same dose; similar cytotoxic mechanisms (i.e., early apoptosis) were observed between treatments, suggesting that co-treatment with JAK2 inhibitors is generally cytotoxic to P-gp-overexpressing resistant cancer cells. Given that fedratinib is FDA-approved, our findings support its application in the co-treatment of P-gp-overexpressing cancer patients showing MDR.
Comprehensive Analysis of Long Noncoding RNA Modified by m6A Methylation in Oxidative and Glycolytic Skeletal Muscles
Authors: Wang Shanshan , Tan Baohua , Xiao Liyao , Zhao Xinming , Zeng Jiekang , Hong Linjun , Yang Jie , Cai Gengyuan , Zheng Enqin , Wu Zhenfang , Gu Ting
Published: April 2022

N6-methyladenosine (m6A) is the most common modification in eukaryotic RNAs. Accumulating evidence shows m6A methylation plays vital roles in various biological processes, including muscle and fat differentiation. However, there is a lack of research on lncRNAs’ m6A modification in regulating pig muscle-fiber-type conversion. In this study, we identified novel and differentially expressed lncRNAs in oxidative and glycolytic skeletal muscles through RNA-seq, and further reported the m6A-methylation patterns of lncRNAs via MeRIP-seq. We found that most lncRNAs have one m6A peak, and the m6A peaks were preferentially enriched in the last exon of the lncRNAs. Interestingly, we found that lncRNAs’ m6A levels were positively correlated with their expression homeostasis and levels. Furthermore, we performed conjoint analysis of MeRIP-seq and RNA-seq data and obtained 305 differentially expressed and differentially m6A-modified lncRNAs (dme-lncRNAs). Through QTL enrichment analysis of dme-lncRNAs and PPI analysis for their cis-genes, we finally identified seven key m6A-modified lncRNAs that may play a potential role in muscle-fiber-type conversion. Notably, inhibition of one of the key lncRNAs, MSTRG.14200.1, delayed satellite cell differentiation and stimulated fast-to-slow muscle-fiber conversion. Our study comprehensively analyzed m6A modifications on lncRNAs in oxidative and glycolytic skeletal muscles and provided new targets for the study of pig muscle-fiber-type conversion.