bluuebunny/crossref_metadata_2025 huggingface dataset:
The effects of different 650 nm laser diode irradiation times on the viability and proliferation of human periodontal ligament fibroblast cells
Authors: Ismiyatin Kun , Subiyanto Ari , Tangdan Ika , Nawawi Rahmi , Lina Reinold C. , Ernawati Rizky , Kurniawan Hendy Jaya
Published: February 2020

Background: Endo-perio lesions are clinical manifestations of inflammation in the periodontal and pulp tissue. Damage to the periodontal ligament can inhibit its ability to regenerate. Therefore, laser therapy use is expected to improve the prognosis with regard to healing lesions. Unfortunately, the duration of irradiation during laser diode therapy can influence the viability and proliferation of human periodontal ligament fibroblast (hPDLF) cells. Purpose: This study aims to determine the effects of different irradiation exposure times of the 650 nm laser diode of the pulsed mode type on the viability and proliferation of human periodontal ligament fibroblast cells. Methods: This study constituted a laboratory experiment on hPDLF cells using 650 nm laser diode irradiation. Six groups formed the research subjects in this study, namely; two control groups, two radiation groups respectively subjected to irradiation exposure of 15 seconds and 35 seconds duration followed by 24-hour incubation, and two radiation groups exposed to irradiation for 15 and 35 seconds respectively followed by 72-hour incubation period. The viability and proliferation of those cells were subsequently calculated by ELISA reader, while the data was analyzed by means of one-way ANOVA and Tukey tests. Results: The significance value of the viability scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, indicating that there was a significant difference between these treatment groups. Similarly, the significance value of proliferation scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, again indicating a significant difference between these treatment groups. Conclusion: Irradiation using a 650 nm laser diode 15 seconds and 35 seconds in duration can induce an increase in the viability and proliferation of hPDLF cells.
Closed reduction in the treatment of neglected mandibular fractures at the Department of Oral and Maxillofacial Surgery, Universitas Airlangga
Authors: Gunardi Olivia Jennifer , Diana Riska , Kamadjaja David Buntoro , Sumarta Ni Putu Mira
Published: February 2020

Background: The mandible is one of the bones most affected by facial fractures commonly resulting from trauma to the face. The ultimate goal of treatment is to re-establish the pre-injury dental occlusion (bite), mandibular anatomy and jaw function of the patient. Treatment approaches range from conservative non-invasive management by ’closed’ reduction and immobilization using intermaxillary fixation (IMF) to the more invasive surgery-based ’open’ reduction incorporating an internal fixation approach. Purpose: The purpose of this case series was to describe the close reduction method as a form of treatment in cases of neglected mandibular fracture. Cases: Four cases of single or multiple mandibular fracture were presented. Case management: All of the cases were managed using a closed reduction method and IMF. Conclusion: A closed reduction method in this case series produced encouraging results and could be considered an alternative in the treatment of neglected mandibular fractures with displacement.
Reccurent trauma-induced aphthous stomatitis in adjustment disorder patients
Authors: Yuliana Y. , Winias Saka , Hendarti Hening Tuti , Soebadi Bagus
Published: February 2020

Background: Adjustment disorder is a temporary psychological condition related to emotional responses or behaviour in reaction to stress resulting from certain changes in a specific period of an individual’s life yet which does not significantly affect his/her daily life. Recurrent aphthous stomatitis (RAS) constitutes recurrent inflammation of the oral mucosa, in the form of an ulcer, frequently associated with psychological stress. Occasionally, a patient does not realise that she/he is suffering from a psychological disorder until the emergence of clinical symptoms, among them recurrent ulcers the causes of which are unknown. Purpose: This article presents a case of adjustment disorder diagnosed from symptoms observable in the oral cavity. Case: The case involved a 21-year-old student who presented with the symptoms of large, painful ulcers on her tongue, the inside of her cheek, and the floor of her mouth. These symptoms had been observable for one month but remained untreated. The patient only ate once a day or even once every two days. An introverted personality, she did not associate with other people. Case management: The procedure covered anamnesis, clinical examination, blood laboratory tests, total immunoglobulin E (IgE), an Antinuclear Antibody (ANA) test, bacterial and fungal culture in ulcus, questionnaire screening on psychological disorders, cortisol level examination and referral to a psychiatrist. Symptomatic therapy administered to the patient led to recovery of the ulcer in 29 days. Conclusion: RAS can be triggered by psychological stress which induces changes in the immune system and oral mucosa tissue.
Peripheral ossifying fibroma of the anterior maxillary gingiva
Authors: Anugraha Ganendra , Sumarta Ni Putu Mira
Published: February 2020

Background: Peripheral ossifying fibroma is a rejuvenation of the reactive gingiva, usually occurring in the anterior maxillary gingiva. The condition is often clinically ambiguous when diagnosed on the basis of gingival hyperplastic lesions such as focal fibrous hyperplasia, peripheral giant cell granuloma, peripheral fibroma and pyogenic granuloma because peripheral ossifying fibroma has a tendency to recur with a ratio of around 20%. The literature on the subject predominantly classifies peripheral osifying fibroma as an epulis type, but it has also been identified as a peripheral mesenchymal tumor presenting similar clinical symptoms to ossified fibrous epulis. Purpose: The purpose of this article is to explain the rare case of peripheral ossifying fibroma in the anterior maxillary gingiva which can be clinically misdiagnosed as reactive gingival hyperplastic lesions. Case: A case report of peripheral ossifying fibroma in the left lateral incisor and canine of the maxillary gingiva in a 26 year-old male. The patient chiefly complained of a painless, slow growing gingival enlargement on the upper left jaw during the previous five years. Clinical examination confirmed it to be a single, hard swelling in the 21-24 region, pale in color and with a rough surface. Case management: The procedure constituted a complete surgical excision of the lesion together with the underlying periosteum curettage intended to prevent recurrence. The histopathologic examination results indicated tissue with squamous epithelial lining, stroma consisting of fibroblasts, and immature trabecula with osteoblastic rimming between collagen tissue without signs of malignancy. Osteoblastic rimming has specific features in histopathologic examination of ossifying fibroma. Conclusion: Peripheral ossifying fibroma is a rare solitary enlargement in the oral cavity frequently misdiagnosed as ossified fibrous epulis. A definitive diagnosis is made by means of histopathologic examination. The condition has a low reccurance rate.
Sistem Informasi Program Pelaporan ISPA di Kota Surabaya
Authors: Mubarokah Nur Fauzia Laily , Notobroto Hari Basuki , Primayanti Primayanti
Published: February 2020

Acute Respiratory Infections (ARI) is a disease that occurs in many developing countries and also on the list of 10 most diseases in hospitals. This study aims to analyze the information system of ARI Reporting Program in Surabaya. Reporting on ARI cases is a surveillance of the disease. If many cases reported, it is better than only a few cases reported. This research is a descriptive study with a qualitative approach. Based on the results of the interview with the informant, it was stated that the ARI reporting program had helped in recording ARI cases that occurred in Surabaya. File formats that are still manual and not integrated with the internet (online) make sending reports longer. Even if you implement an integrated online, data entry only needs to be done once so that the same data will be stored electronically and can be sent and processed. In addition, in the excel file ARI reporting program, there are several output menus that can be used as a result of the description of ARI cases. However, the output was underutilized so that less case monitoring was implemented to reduce the number of ARI / Pneumonia cases that occurred in Surabaya. The conclusion is the ARI reporting program is very helpful in the process of data collection of ARI cases, but it would be better if they use the type of online computerization management and better utilize the graphical output of the ARI reporting program.
Faktor yang Mempengaruhi Terjadinya Pernikahan Usia Dini
Authors: Angraini Wulan , Pratiwi Bintang Agustina , Febriawati Henni , Yanuarti Riska , Anita Betri , Oktarianita Oktarianita
Published: February 2020

Bengkulu Tengah is one of the Regencies in Bengkulu Province with the highest number of marriages in the early age compared to the other nine regencies/cities, which amounted to 29.12 percent. This study aims to determine the factors that influence early marriage in Bengkulu Tengah District. This type of research is quantitative, the population were all married couples in January-September 2017. Sampling using Propotional Random Sampling technique with a total sample of 237 people. Data was collected using a valid and reliable questionnaire then analyzed using univariate and multivariabel analysis (Multiple Linear Regression Test). The result show that youngest age of marriage is 15 years, lowest income Rp 100,000, youngest menarche age 10 years, average knowledge score 6.19, attitude 29.43, media exposure 1.63, influence of friend 5, parental support 2.23, stigma 4.29. The factors that influence the occurrence of early marriage are knowledge, Age of Menarche, and Media (p value < 0.05). The age of Menarche is the most influential factor in the age of marriage. The younger the age of menarche, the younger the age of one's marriage. It is better for teens who have experienced menstruation to maintain their reproductive health by delaying marriage in their teens.
Mental health of disabled children: a preventive aspect
Authors: Kupriyanova I. E. , Dashieva B. A. , Karaush I. S.
Published: February 2020

The aimis to identify the priority directions for preventing mental disorders in disabled children following a comprehensive analysis of clinical, social, psychological, ethnic and cultural characteristics over 2009– 2017.Materials and methods. 2,204 people were included in the study: disabled children, students of remedial schools aged 7–18 years old (n = 834); parents of disabled children (n = 772); teachers working in various educational facilities (n = 217); respondents of the survey identifying people’s attitude towards children with disabilities (schoolchildren, students, adults, n = 381). Clinical, psychological and statistical methods were used in the study. To identify depression and anxiety in children, Children’s Depression Inventory (CDI), Childhood Myositis Assessment Scale (CMAS), PedsQL inventory, survey on coping strategies (E. Heim) and adapted questionnaires for suicidal risk and alexithymia were used. For adult participants, the Quality of Life Scale, Hamilton Anxiety Rating Scale, Hospital Anxiety and Depression Scale (HADS), and a questionnaire identifying the attitude towards children with disabilities were applied.

Results. A high prevalence of mental disorders in children with disabilities (62.7–95.2%) was identified, and the levels of anxiety, suicidal risk, alexithymia were determined. Children’s families were characterized by impaired structure (50%), parenting styles resulting in pathologies (71.5%), low income level (60%), low qualifications of parents and unemployment (13%), alcohol abuse (17.6%), victimized attitudes and low satisfaction with the psychological criteria of quality of life, combined with low level of anxiety. For teachers, the levels of anxiety and depression and signs of burnout were revealed, and the features of their interaction with disabled children in the educational process were described. The attitude of different social groups to children with disabilities was studied. The methodological conditions for carrying out research and organizing medical and psychological care were formulated.

Conclusion. Prevention of mental disorders in disabled children includes identification of early signs of anxiety, depression, suicidal risk and assessment of the quality of life and work. This is ensured by a reasonable choice of research methods, psychosocial rehabilitation with involvement of beloved people, and increase in the tolerance level towards children with disabilities in the society.
Adicciones: viejas terapias grupales (TG) y nuevos pacientes
Authors: Monras Miquel , Ortega Lluisa , Mondon Silvia , Lopez-Lazcano Ana , Lligoña Anna
Published: February 2020

La comorbilidad psiquiátrica y la variabilidad y frecuencia del policonsumo de drogas ha aumentado (un 75% de la muestra), lo que dificulta el uso de enfoques e instrumentos clásicos como la TG y que los profesionales vamos a remolque intentando adaptarlos a estas nuevas realidades. Pero esta adaptación es posible y positiva y pasa por: i) flexibilizar las indicaciones de TG mediante una valoración más individualizada, posiblemente en un dispositivo de hospital de día, de los pacientes con TP, trastornos cognitivos o con consumo activo de alguna droga secundaria, ii) adecuado control psicoterapéutico de estos pacientes con mayor riesgo de recaída y de afectar negativamente las dinámicas grupales, iii) mayor formación de los terapeutas para poder manejar a estos pacientes, iv) creación en algunos casos de grupos específicos para pacientes con características y objetivos específicos, como son los pacientes que mantienen consumos activos de alcohol (porque quieren beber o porque no pueden evitarlo), o los mayores de 60 años, muchos con deterioro cognitivo.
Public Green Space and Mental Health building Self-Esteem for Elderly Population in Dhaka
Authors: Rahman K M Atikur , Meiyu Pan , Shorkar Md Abu Naim
Published: February 2020

Green public space immensely provides ecological soundness (ES), social resilience, social inclusiveness, mental health, self-esteem, and cultural prosperity from privileged class to lower ones. This research aims at estimating the effect of social services, safety, and infrastructural amenity in green space (parks) on mental health building self-esteem for elderly population. Data were collected from four parks out of 21 in Dhaka selected by systematic random sampling. Two-hundred and three elderly participants were picked up for survey using snowball sampling. The survey was conducted in a pre-structured questionnaire consisted of 40 close-ended questions. Data was analyzed applying ordinal logistic model, multivariate regression analysis, and Partial Least Square (PLS) -Path Modeling. The study explored several key findings-1) Infrastructural amenities impacted social services of parks; 2) Social services contributed positively to mental health boosting up self-esteem for elderly population. 3) Likewise, mental health for elderly population was affected by safety measure of green space. Dhaka requires constructing age-friendly parks to promote elderly mental health.
An Alternative to PCA for Estimating Dominant Patterns of Climate Variability, with Application to US Rainfall
Authors: Jewson Stephen
Published: February 2020

Floods and droughts are driven, in part, by patterns of extreme rainfall. Heat waves are driven, in part, by patterns of extreme temperature. The standard work-horse for understanding patterns of climate variability is Principal Component Analysis (PCA) and its variants. But PCA does not optimize for spatial extremes, and so there is no particular reason why the first PCA pattern should identify, or even approximate, the types of patterns that may drive these phenomena, even if the linear assumptions underlying PCA are correct. We present an alternative pattern identification algorithm that makes the same linear assumptions as PCA, but which can be used to explicitly optimize for spatial extremes. We call the method Directional Component Analysis (DCA), since it involves introducing a preferred direction, or metric, such as `sum of all points in the field'. We compare the first PCA and DCA patterns for US rainfall on a 6 month timescale, using the sum metric for the definition of DCA, and find that they are somewhat different. The definitions of PCA and DCA mean that the first PCA pattern has the larger explained variance of the two, while the first DCA pattern, when scaled appropriately, is both more likely and captures more rainfall. In combination these two patterns yield more insight into rainfall variability than either pattern on its own.
Calculation of the Isobaric Heat Capacities of the Liquid and Solid Phase of Organic Compounds at 298.15K by Means of a Generally Applicable Computer Algorithm Based on the Group-Additivity Method
Authors: Naef Rudolf
Published: February 2020

The calculation of the isobaric heat capacities of the liquid and solid phase of molecules at 298.15 K is presented, applying a universal computer algorithm based on the atom-groups additivity method, using refined atom groups. The atom groups are defined as the molecules' constituting atoms and their immediate neighbourhood. In addition, the hydroxy group of alcohols are further subdivided to take account of the different intermolecular interactions of primary, secondary and tertiary alcohols. The evaluation of the groups' contributions has been carried out by means of a fast Gauss-Seidel fitting calculus using experimental data from literature. Plausibility has been tested immediately after each fitting calculation using a 10-fold cross-validation procedure. For the heat capacity of liquids, the respective goodness of fit of the direct (R2) and the cross-validation calculations (Q2) of 0.998 and 0.9975, and the respective standard deviations of 8.2 and 9.16 J/mol/K, together with a medium absolute percentage deviation (MAPD) of 2.69%, based on the experimental data of 1133 compounds, proves the excellent predictive applicability of the present method. The statistical values for the heat capacity of solids are only slightly inferior: for R2 and Q2, the respective values are 0.9915 and 0.9875, the respective standard deviations are 12.19 and 14.13 J/mol/K and the MAPD is 4.65%, based on 732 solids. The predicted heat capacities for a series of liquid and solid compounds has been directly compared to those received by a complementary method based on the "true" molecular volume [1] and their deviations elucidated.
The Microbiota-gut-immune-glia (MGIG) Axis in Major Depression
Authors: Rudzki Leszek , Maes Michael
Published: February 2020

There is robust evidence that major depression (MDD) is accompanied by a low-grade activation of the immune-inflammatory response system, which is involved in the pathophysiology of this disorder. It is also becoming apparent that glia cells are in reciprocal communication with neurons and orchestrate various neuromodulatory, homeostatic, metabolic, and immune mechanisms and have a crucial role in neuroinflammatory mechanisms in MDD. Those cells mediate the central nervous system (CNS) response to systemic inflammation and psychological stress, but at the same time, they may be an origin of the inflammatory response in the CNS. The sources of activation of the inflammatory response in MDD are immense, however, in recent years, it is becoming increasingly evident that the gastrointestinal tract with gut-associated lymphoid tissue (GALT) and increased intestinal permeability to bacterial LPS and food-derived antigens contribute to activation of low-grade inflammatory response with subsequent psychiatric manifestations. Furthermore, an excessive permeability to gut-derived antigenic material may lead to subsequent autoimmunities which are also known to be comorbid with MDD. In this chapter, we discuss fascinating interactions between the gastrointestinal tract, increased intestinal permeability, intestinal microbiota, and glia-neuron crosstalk, and their roles in the pathogenesis of the inflammatory hypothesis of MDD. To emphasize those crucial intercommunications for the brain functions, we propose the term of microbiota-gut-immune-glia (MGIG) axis.
Study on Coupling Coordination Relationship Between Water Use Efficiency and Economic Development
Authors: Xu Wen-Jie , Zhang Xiao-Ping , Xu Qian , Gong Hui-Ling , Li Qing , Liu Bo , Zhang Jing-Wei
Published: February 2020

In order to realize the sustainable utilization of water resources and the sustainable development of economy, the evaluation index system of water use efficiency system and economic development system was constructed. Entropy weight method and comprehensive evaluation method were adopted to determine the index weights and conduct comprehensive evaluation for the two systems. The coupling coordination model was used to calculate the coupling degree, coordination degree and coupling coordination degree of the two systems. The annual coupling stage and coupling coordination intensity of the two systems were analyzed and determined. The results showed that the comprehensive evaluation values of both water use efficiency system and economic development system in Jinan City increased greatly from 2008 to 2017, and showed a changing trend of ups and downs. The two systems were in the antagonistic stage, and were gradually approaching the running-in stage, indicating that they were in the state of common development. The coupling coordination degree of the two systems gradually increased in waves, progressed from the moderately to the highly coordinated coupling. In the future, if the water control path appropriate to Jinan City can be explored actively, the water-saving kinetic energy of economic development can be increased constantly, and the linkage effect between economic development and water resources utilization can be given full play to, the two systems will be in the orbit with the benign interaction and healthy harmonious development.
Reciprocal Heterospecific Pollen Interference among Alien and Native Species
Authors: Malecore Eva M. , Berthelot Sylvie , van Kleunen Mark , Razanajatovo Mialy
Published: February 2020

1. Heterospecific pollen interference has recently been proposed as a mechanism contributing to the success of alien invaders, as heterospecific pollen of alien plants interferes with the reproduction of natives by reducing fruit and seed set. However, no study has looked at the opposite interaction. Moreover, few studies have considered the roles of phylogenetic and trait distances between pollen donors and recipients. 2. We did a large multi-species experiment in which we used alien and native species both as pollen recipients and as pollen donors, and included phylogenetic as well as trait distance as explanatory variables. 3. We found that both alien and native recipients suffered from heterospecific pollen from donors of the opposite status in terms of seed and fruit set. Phylogenetic distance and trait distance both affected heterospecific pollen interference, but the effect depended on recipient and donor statuses. 4. We conclude that heterospecific pollen interference affects both native and alien recipients, thus indirectly altering community composition and increasing biotic resistance against invaders.
A Low Profile Wideband Antenna for WWAN/LTE Applications
Authors: Azim Rezaul , Affandi Adnan , Alam M Mottahir , Islam M Tariqul
Published: February 2020

In this paper a low profile antenna is presented for wideband communication applications. The presented design is consists of an I-shape driven strip and a rectangular ground strip with an open slot in the middle and steeped lower portion. Measured result demonstrates that the achieved operating band of the proposed antenna has the potential to cover Global Star Satellite Phones (1.61-1.63 GHz, uplink), Advanced Wireless Systems (1.71-1.76 GHz, 2.11-2.17 GHz), DCS (1710-1880 MHz), GSM (1800MHz), DCP (1.88-1.90 GHz), DCS-1900/PCS/PHS (1850-1990MHz), WCDMA/IMT-2000 (1920-2170MHz), UMTS (1920-2170 MHz), LTE bands (FDD LTE bands 1-4, 9-10, 15-16, 23-25, 33-37, 39). The designed antenna possessed a very small size of 0.35λ0 × 0.027λ0 at the lowest frequency (S11 ≤ -10dB) and achieved good gain, exhibits stable radiation patterns which make it suitable for handheld communication devices.
Restricticin B from the Marine Fungus Penicillium janthinellum and Its Inhibitory Activity on the NO Production in BV-2 Microglia Cells
Authors: Choi Byeonung-Kyu , Park Shin-Young , Jo Song-Hee , Choi Dong-Kug , Trinh Phan Thi Hoai , Lee Hwa-Sun , Cao Van Anh , Ngo Thi Duy Ngoc , Van Tran Thi Thanh , Shin Hee Jae
Published: February 2020

A new compound containing a triene, a tetrahydropyran ring and glycine ester functionalities, restricticin B (1), together with four known compounds (2-5) were obtained from the EtOAc extract of the marine fungus Penicillium janthinellum. The planar structure of 1 was determined by detailed analyses of MS, 1D and 2D NMR data. The relative and absolute configurations of 1 were established via analyses of NOESY spectroscopy data, comparison of optical rotation values with those of restricticin derivatives reported and electronic circular dichroism (ECD). All the compounds were screened for their anti-neuroinflammatory effects in lipopolysaccharide (LPS)-induced BV-2 microglia cells. Restricticin B (1) and N-acetyl restricticin (2) exhibited anti-neuroinflammatory effects by suppressing the production of pro-inflammatory mediators in activated microglial cells.
Preoperative anemia and hospitalization time are the independent factors of preoperative deep venous thromboembolism in Chinese elderly undergoing hip surgery
Authors: Author) long feng(Former Corresponding , Xu Longhe , Yuan Weixiu , Xu Zhipeng , Author) ZeGuo Feng(New Corresponding , Zhang Hong
Published: February 2020

Aim This study was designed to explore the prevalence condition and risk factors of preoperative deep venous thromboembolism (DVT) in Chinese elderly with hip fracture. Methods From January 1, 2012, to December 31, 2018, 273 elderly over 70 years old with elective hip surgery were collected from the electronic medical records. Collected Data included demographic characteristics, comorbidities, ASA classification, types of surgeries, types of anesthesia, operation time, fracture to operation time, perioperative blood transfusion, preoperative hemoglobin level, anemia, blood-gas analysis, cardiac function, electrocardiograph, lower limb venous ultrasonography and hospitalization time. Results In all patients, 15 patients (5.6%) had ultrasonography evidence of DVT in affected limbs before surgery. Three of all patients received an temporary inferior vena cave filter placement. Fracture to surgery time, preoperative hemoglobin level, anemia, pulmonary disease and hospitalization time were statistically different between DVT group and non-DVT group (P<0.05 for all). Moreover, preoperative anemia (OR: 0.144, 95%CI: 0.026-0.799, P=0.027) and hospitalization time (OR: 1.135; 95%CI: 1.023-1.259, P=0.017) were the two independent risk factors for preoperative DVT. Conclusion Preoperative anemia and hospitalization time were independent risk factors for venous DVT in Chinese elderly with hip fracture.
Management and Prognostic Prediction of appendiceal mucinous adenocarcinoma with peritoneal metastasis: a single center study in China
Authors: Ma Ruiqing , Wang Bing , Zhai Xichao , Lu Yiyan , Xu Hongbin
Published: February 2020

Background To investigate the clinical and pathological characteristics of appendiceal mucinous adenocarcinoma with peritoneal metastasis and analyze the prognostic factors. Methods A retrospective analyses of clinicopathological features of 50 patients with appendiceal mucinous adenocarcinoma with peritoneal metastasis from January, 2013 to December, 2017 in Aerospace Central Hospital, Beijing, China. Survival data calculation and comparison were respectively performed with the Kaplan-Meier method and the log-rank test. The Cox proportional hazards regression method was used for multivariate survival analyses. Results Cytoreduction for appendiceal mucinous adenocarcinoma was conducted on 50 patients (24 males and 26 females), with a median age of 52.5 years at the time of surgery (range 31-71 years). The median overall survival (OS) time was 24 months, with 2-,3- and 5-year survival rates of 53%, 24% and 8%, respectively. At the last follow-up in December 2018, 13 patients were still alive. Multivariate analysis revealed that patients who had low Ki-67 expression (less than 50%) and CCR (completeness of cytoreduction) 0/1/2 score had significantly better OS rate than their respective counterparts. Conclusions Ki-67 expression statue and CCR score could be employed as the prognosis prediction in patients with appendiceal mucinous adenocarcinoma.
Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila
Authors: Najafi Hadi , Wong Kyle , Kim Woo Jae
Published: February 2020

Background Drosophila melanogaster is a proper model organism for studying the development and function of the nervous system. The Drosophila nervous system consists of distinct cell types with significant homologies to various cell types of more advanced organisms, including human. Among all cell types of the nervous system, astrocyte-like glia (ALG) have conserved functions to mammals and are essential for normal physiology and behaviours of the fly. Results In this study, we exploited the gene expression profile of single cells in Drosophila optic lobe to identify the genes with specific expression pattern in each cell type. Through a bioinformatical analysis of the data, a novel ALG-specific gene (here assigned as deathstar ) was identified. Immunostaining of deathstar in the central nervous system (CNS) showed its presence in specific regions of Drosophila ventral nerve cord, which previously has been characterized as ALG cells. Consistent with the bioinformatical analysis, deathstar -related signals were overlapped with the signals of the previously-reported ALG marker, Eaat1 , supporting its specific expression in ALG cells. At the physiological level, RNAi-mediated suppression of deathstar gene impeded the normal development of male flies without any effects on females. Cell type-specific expression of deathstar RNAi showed that deathstar gene affects locomotion behaviour and lifespan of D. melanogaster , in an ALG-specific manner. Conclusions Taken together, we showed that bioinformatical analysis of a previously reported expression data of Drosophila optic lobe successfully predicted the ALG-specific expression pattern of deathstar gene. Moreover, it was consistent with the ALG-specific effects of this gene on locomotion and lifespan of D. melanogaster, in vivo .
Retrospective analysis of salvage surgery for local progression of brain metastasis previously treated with stereotactic irradiation: diagnostic contribution, functional outcome, and prognostic factors
Authors: Mitsuya Koichi , Nakasu Yoko , Deguchi Shoichi , Oishi Takuma , Sugino Takashi , Yasui Kazuaki , Ogawa Hirofumi , Onoe Tsuyoshi , Asakura Hirofumi , Harada Hideyuki , Hayashi Nakamasa
Published: February 2020

Background: Stereotactic irradiation (STI) is a primary treatment for patients with newly diagnosed brain metastases. Some of these patients experience local progression, which is difficult to differentiate from radiation necrosis, and difficult to treat. So far, just a few studies have clarified the prognosis and effectiveness of salvage surgery after STI. We evaluated the diagnostic value and improvement of functional outcomes after salvage surgery. Based on these results, we reconsidered surgical indication for patients with local progression after STI.

Methods: We evaluated patients with brain metastases treated with salvage surgery for local progression from October 2002 to July 2019. These patients had undergone salvage surgery based on magnetic resonance imaging findings and/or clinical evidence of post-STI local progression and stable systemic disease. We employed two prospective strategies according to the eloquency of the lesions. Lesions in non-eloquent areas had been resected completely with a safety margin, utilizing a fence-post method; while lesions in eloquent areas had been treated with minimal resection and postoperative STI. Kaplan-Meier curves were used for the assessment of overall survival. Prognostic factors for survival were analyzed.

Results: Fifty-four salvage surgeries had been performed on 48 patients. The median age of patients was 63.5 years (range 36-79). The median interval from STI to surgery was 12 months. The median overall survival was 20.2 months from salvage surgery and 37.5 months from initial STI. Primary cancers were lung 31, breast 9, and others 8. Local recurrence developed in 13 of 54 lesions (24%). Leptomeningeal dissemination occurred after surgery in 3 patients (5.6%). Primary breast cancer (breast vs. lung: HR: 0.17), (breast vs. others: HR: 0.08) and RPA class 1-2 (RPA 1 vs. 3, HR:0.13), (RPA 2 vs 3, HR:0.4) were identified as good prognostic factors for overall survival (OS) in multivariate analyses. The peripheral neutrophil-to-lymphocyte ratio (NLR) of ≤3.65 predicted significantly longer OS (median 25.5 months) than an NLR >3.65 (median 8 months).

Conclusion: We insist that salvage surgery leads to rapid improvement of neurological function and clarity of histological diagnosis. Salvage surgery is recommended for large lesions especially with surrounding edema either in eloquent or non-eloquent areas.
Microbial and inflammatory parameters during the process of non-surgical treatment of Peri-implantitis: Topical minocycline application
Authors: Shwe Yee Mon , Inoue Kensuke , Yamamoto Maiko , Hidemi Nakata , Kuroda Shinji , Kasugai Shohei
Published: February 2020

The authors have withdrawn this preprint from Research Square.
Effect of the Polaris Loop ureteral stent on vesicoureteral reflux and relief of lumbago symptoms: a single-center study
Authors: Yan Wei , Qiao Ludong , Du Zhen , Guan Di , Ping Hao , Chen Shan
Published: February 2020

Objective

To verify the effect of the Polaris Loop ureteral stent on vesicoureteral reflux and relief of lumbago symptoms in patients after ureteroscopic procedures.

Methods

This was a prospective single-center analysis of 20 patients who received indwelling bilateral ureteral stents after undergoing ureteroscopic lithotripsy. The Polaris Loop stent and Polaris Ultra stent (both F6 and 26 cm) were implanted randomly on the left and right sides of a patient. The results of the visual analogue scale (VAS) of the symptoms of lumbar discomfort that occurred within 2 weeks after surgery and whether or not vesicoureteral reflux occurred during postoperative cystography were recorded.

Results

The differences between the VAS scores and rates of vesicoureteral reflux for the 2 types of stents were significant (p<0.05). The patients receiving the Polaris Loop stent had lower VAS scores and lower rates of vesicoureteral reflux than the patients receiving the Polaris Ultra stent.

Conclusions

The Polaris Loop stent led to improved patient comfort after ureteroscopy and prevented vesicoureteral reflux of urine. However, careful selection of the appropriate length of stent and appropriate placement technique is required.
Engaging Stakeholders in the Adaptation of the Connect for Health Pediatric Weight Management Program for National Implementation
Authors: Simione Meg , Frost Holly M. , Cournoyer Rachel , Mini Fernanda Neri , Cassidy Jackie , Craddock Cassie , Moreland Jennifer , Wallace Jessica , Metlay Joshua , Kistin Caroline J. , Sease Kerry , Hambidge Simon J. , Taveras Elsie M.
Published: February 2020

Background: Connect for Health is an evidence-based weight management program with clinical- and family-facing components for delivery in pediatric primary care for families of children ages 2 to 12 years. We used the Consolidated Framework for Implementation Research (CFIR) to guide formative work prior to national implementation. The purpose of this study was to describe the process and results of stakeholder engagement and program adaptation.Methods: We used mixed qualitative and quantitative methods to iteratively adapt and optimize the program by assessing needs and perspectives of clinicians and parents, as well as contextual barriers, facilitators and organizational readiness for the uptake of the proposed program tools and implementation strategies. We conducted interviews with primary care clinicians from four health care organizations in Boston, MA, Denver, CO, and Greenville, SC and used principles of immersion-crystallization for qualitative analyses. We also conducted surveys of parents of children with a body mass index ≥ 85th percentile.Results: We reached thematic saturation after 52 clinician interviews. Emergent themes representing the CFIR domains of intervention characteristics, outer and inner setting, and process included: (1) importance of evidence-based clinical decision support tools that integrate into the workflow and do not extend visit time; (2) developing resources that respond to family’s needs; (3) using multimodal delivery options for family resources; (4) addressing childhood obesity while balancing competing demands; (5) emphasizing patient care rather than documentation and establishing sustainability plans; and (6) offering multiple training methods that incorporate performance feedback. Of the parents surveyed (n=400), approximately 50% were Spanish-speaking and over 75% reported an annual income < $50,000. Parents affirmed the importance of addressing weight management during well-child visits, being provided with referrals and resources, and offering multiple methods for resource delivery. Decisions about program modifications were made at the program and healthcare-system level and based on stakeholder engagement findings. Modifications included cultural, geographic, and target audience adaptations, as well as varied resource delivery options.Conclusions: To ensure the fit between the Connect for Health program and national implementation settings, adaptations were systematically made through engagement of clinician and parent stakeholders to support adoption, sustainability, and health outcomes.
Clinical difference between acute appendicitis and acute right-sided colonic diverticulitis
Authors: Song Ji Ho , Lee Sanghun , Ho Han , Seo Jun Seok , Lee Jeong Hun , Lee Seung Chul , Kim Yong Won
Published: February 2020

Background: The clinical presentations of acute appendicitis (AA) and acute right-sided colonic diverticulitis (ARCD) are similar, but the usual treatment for each disease differs between surgical and conservative management. This study aimed to identify the clinical differences between AA and ARCD.Method: We performed a single-center retrospective case-control study on adult patients with AA and ARCD confirmed by computed tomography who had visited an emergency department between March 2018 and February 2019. The clinical variables, including past medical history, presented symptoms and signs, and laboratory findings were compared between the two groups. We subsequently performed a logistic regression analysis for differentiating ARCD from AA based on the results of univariate analyses.Results: A total of 222 (79%) and 59 (21%) patients were enrolled in the AA and ARSD groups, respectively. Logistic regression analysis revealed that factors associated with ARCD were a past history of diverticulitis [OR 141.691 (95% CI: 12.222 – 1642.601), p < 0.001], ketonuria [OR 0.268 (95% CI: 0.099 – 0.726), p = 0.010], anorexia [OR 0.037 (95% CI: 0.007 – 0.207), p < 0.001], and neutrophilia [OR 0.179 (95% CI: 0.062 – 0.519), p = 0.002].Conclusion: Anorexia, neutrophilia, and ketonuria were predictors of AA and a history of diverticulitis was a predictor of ARCD.
Elevated Plasmodium infection and multiple insecticide resistance in the principal malaria vectors Anopheles funestus and Anopheles gambiae in a forested locality close to the airport of Yaoundé, the Capital city of Cameroon
Authors: Nongley Nkemngo Francis , Mbugulize Jean Leon Mugenzi , Terence Ebai , Niang Abdoulaye , Wondji Murielle , Tchoupo Micareme , Nguete Daniel Nguiffo , Tchapga Williams , Irving Helen , Ghogomu Stephen M. , Ndo Cyrille , Consortium COMAL , Borrmann Steffen , Wondji Charles S.
Published: February 2020

Background Reducing the burden of malaria requires better understanding of vector populations particularly in forested regions where the incidence remains elevated. Here, we characterized malaria vectors in a locality near the international airport of Yaoundé, the capital city of Cameroon, including species composition, abundance, Plasmodium infection rate; insecticide resistance profiles and underlying resistance mechanisms. Methods Blood-fed adult mosquitoes resting indoors were aspirated from houses in April 2019 at Elende, a village located 2km from the Yaoundé-Nsimalen airport. Female mosquitoes were forced to lay eggs to generate F 1 adult progeny. WHO bioassays were performed to assess resistance profile to insecticides. The threshold of insecticide susceptibility was fixed above 98% mortality rate and mortality rates below 90% were considered indicative of confirmed insecticide resistance according to the WHO test procedures for insecticide resistance monitoring. Furthermore, the molecular basis of resistance and Plasmodium infection rates were investigated.Results Anopheles funestus s.s. was most abundant species in Elende (85%) followed by Anopheles gambiae s.s. (15%) with both having a similar sporozoite infection rate. Both species exhibited high levels of resistance to pyrethroids (<40% mortality). An. gambiae s.s. was also resistant to DDT (9.9% mortality) and bendiocarb (54% mortality) while susceptible to organophosphate. An. funestus s.s. was resistant to dieldrin (1% mortality), DDT (86% mortality) but susceptible to carbamates and organophosphates. The DDT/pyrethroid L119F-GSTe2 resistance allele (8%) and G119S ace -1 resistance allele (15%) were detected in An. funestus s.s. and An. gambiae s.s. respectively. Furthermore, the high pyrethroid/DDT resistances in An. gambiae s.s. corresponded with an increase frequency of 1014F kdr allele (95%). The CYP6P9a P450 resistance allele was absent in the An. funestus s.s. population meanwhile transcriptional profiling of candidate cytochrome P450 genes reveals the over-expression of CYP6P5 , CYP6P9a and CYP6P9b. Conclusion The resistance to multiple insecticide classes observed in these vector populations alongside the high Plasmodium infection rate highlights the challenges that public health vector control programs encounter in sustaining the regular effectiveness of contemporary insecticide-based control interventions in forested areas. More particularly, the resistance observed against the carbamates and possible resistance against the organophosphates constitutes a major concern for IRS.
Radiation increases BTB permeability in a preclinical model of breast cancer brain metastasis.
Authors: Sprowls Samuel Adam , Arsiwala Tasneem A , Kielkowski Brooke N , Pizzuti Vincenzo , Siochi R. Alfredo C. , Lockman Paul R
Published: February 2020

Background Brain metastasis is a devastating stage of cancer progression, occurring in ~30% of metastatic breast cancer patients. Two-year survival rates for these patients is low, and most typically survive less than one year. Treatments for these women are limited by the blood-brain barrier, but include cytotoxic chemotherapy, surgical resection, and radiation therapy (whole-brain radiotherapy or stereotactic radiosurgery). Radiotherapy is considered to be capable of inducing disruption of the blood-brain barrier and eliciting an abscopal response to extracranial tumors. Methods A combination of ionization chamber and Gafchromic film dosimetry was used to commission and determine dose outputs for our experimental design. Dose deposition in-vivo was verified by immunohistochemistry. To evaluate the effects of ionizing radiation at the normal blood-brain barrier and the blood-tumor barrier, athymic nude and FVB mice were used. Athymic nude mice were injected with MDA-MB-231Br cells. Lesions were allowed to develop for ~28 days. Mice were then irradiated at the prescribed dose. Prior to tissue collection, mice were injected with Texas red, followed by a vascular washout with physiological buffer. Fluorescence in normal and diseased brain was quantified by fluorescent microscopy. Results Using a 10mmx10mm collimator, determined to have adequate field homogeneity as determined by Gafchromic film analysis, we were able to successfully treat a single hemisphere in mice. The blood-brain-barrier remained undisrupted in athymic nude mice at doses up to 12Gy compared to untreated brain and radiation naïve controls. Immune competent FVB mice treated with radiation showed significant blood-brain barrier disruption at a dose of 12Gy only. The blood-tumor barrier showed significant disruption at 24hrs following radiation treatment (6 or 12Gy). Conclusion Our study demonstrated that radiation therapy disrupts the blood-tumor barrier, but fails to disrupt the normal blood-brain barrier in athymic nude mice. However, in FVB immune competent mice, the blood-brain barrier was disrupted at a dose of 12Gy, suggesting an abscopal-like response impacts extent of barrier leakage.
MDC1 depletion promotes Cisplatin induced cell death in Cervical cancer cells
Authors: Singh Neeru , Bhakuni Rashmi , Chhabria Dimple , Kirubakaran Sivapriya
Published: February 2020

Objective: Cisplatin, the most common chemotherapeutic drug for the treatment of advanced stage cervical cancers has limitations in terms of drugs resistance observed in patients partly due to functional DNA damage repair (DDR) processes in the cell. Mediator of DNA damage checkpoint1 (MDC1) is an important protein in the Ataxia telangiectasia mutated (ATM) mediated double stranded DNA break (DSB) repair pathway. In this regard, we investigated the effect of MDC1change in expression on the cisplatin sensitivity in cervical cancer cells.Results: Through modulation of MDC1 expression in the cervical cancer cell lines; Hela, SiHa and Caski, we found that all the three cell lines silenced for MDC1 exhibited higher sensitivity to cisplatin treatment with inefficiency in accumulation of p γH2AX, Ser 139 foci and increased accumulation of pChk2 Thr 68 at the damaged chromatin followed by enhanced apoptosis. Further, we observed the increased p53 Ser 15 phosphorylation in the MDC1 depleted cells. Our studies suggest that MDC1 expression could be a key determinant in cervical cancer prognosis and treatment and its depletion in combination with cisplatin has the potential to be explored for the sensitisation of chemo-resistant cervical cancer cells.