bluuebunny/crossref_metadata_2025 huggingface dataset:
Flexible wavelength-, pulse-controlled mode-locked all-fiber laser based on a fiber Lyot filter
Authors: Han Dongdong , Mei Lingzhi , Hui Zhanqiang , Soliman Abdel-Hamid , Ren Kaili , Zheng Yipeng , Li Tiantian , Gong Jiamin
Published: October 2022

In this paper, we report a flexible wavelength-, pulse-controlled mode-locked all-fiber laser based on a novel fiber optic Lyot filter. The wavelength, pulse duration and spectral bandwidth of passive mode-locked lasers can be tuned by controlling the polarization controller. The proposed Lyot filter was constructed by a single-mode fiber insertion between two polarization-maintaining fibers. The filter bandwidth and laser output tunability were based on the birefringence characteristics of the polarization-maintaining fibers. This all-fiber laser is simple and stable and can be used for various applications where width-tunable or wavelength-tunable pulses are necessary.
Two single-axis scanners make a virtual gimbaled scanner
Authors: Liu Yehe , Jenkins Michael W.
Published: October 2022

A 2D scan generated from two single-axis scanning mirrors often has the beam steered about two distant axes that lead to scan artifacts, such as displacement jitters, telecentric errors, and spot variations. Previously, this problem has been addressed with complicated optical and mechanical designs, such as 4f relays and gimbaled mechanics, which ultimately limit the performance of the system. Here, we show that two single-axis scanners alone can produce a 2D scanning pattern nearly identical to a single-pivot gimbaled scanner through an apparently previously undiscovered simple geometry. This finding broadens the design parameter space of beam steering applications.
Turbulence-resilient pilot-assisted self-coherent free-space optical communications using a photodetector array for bandwidth enhancement
Authors: Song Hao , Zhang Runzhou , Zhou Huibin , Su Xinzhou , Zou Kaiheng , Duan Yuxiang , Karapetyan Narek , Song Haoqian , Pang Kai , Hu Nanzhe , Minoofar Amir , Tur Moshe , Willner Alan E.
Published: October 2022

We experimentally demonstrate a 4-Gbit/s 16-QAM pilot-assisted, self-coherent, and turbulence-resilient free-space optical link using a photodetector (PD) array. The turbulence resilience is enabled by the efficient optoelectronic mixing of the data and pilot beams in a free-space-coupled receiver, which can automatically compensate for turbulence-induced modal coupling to recover the data’s amplitude and phase. For this approach, a sufficient PD area might be needed to collect the beams while the bandwidth of a single larger PD could be limited. In this work, we use an array of smaller PDs instead of a single larger PD to overcome the beam collection and bandwidth response trade-off. In the PD-array-based receiver, the data and pilot beams are efficiently mixed in the aggregated PD area formed by four PDs, and the four mixing outputs are electrically combined for data recovery. The results show that: (i) either with or without turbulence effects (
D
/
r
0 = ∼8.4), the 1-Gbaud 16-QAM signal recovered by the PD array has a lower error vector magnitude than that of a single larger PD; (ii) for 100 turbulence realizations, the pilot-assisted PD-array receiver recovers 1-Gbaud 16-QAM data with a bit-error rate below 7% of the forward error correction limit; and (iii) for 1000 turbulence realizations, the average electrical mixing power loss of a single smaller PD, a single larger PD, and a PD array is ∼5.5 dB, ∼1.2 dB, and ∼1.6 dB, respectively.
Distance calibration via Newton’s rings in yttrium lithium fluoride whispering gallery mode resonators
Authors: Christensen Josh T. , Azeem Farhan , Trainor Luke S. , Strekalov Dmitry V. , Schwefel Harald G. L.
Published: October 2022

In this work, we analyze the first whispering gallery mode resonator (WGMR) made from monocrystalline yttrium lithium fluoride (YLF). The disc-shaped resonator is fabricated using single-point diamond turning and exhibits a high intrinsic quality factor (Q) of

8
×

10
8

. Moreover, we employ a novel, to the best of our knowledge, method based on microscopic imaging of Newton’s rings through the back of a trapezoidal prism. This method can be used to evanescently couple light into a WGMR and monitor the separation between the cavity and the coupling prism. Accurately calibrating the distance between a coupling prism and a WGMR is desirable as it can be used to improve experimental control and conditions, i.e., accurate coupler gap calibration can aid in tuning into desired coupling regimes and can be used to avoid potential damage caused by collisions between the coupling prism and the WGMR. Here, we use two different trapezoidal prisms together with the high-Q YLF WGMR to demonstrate and discuss this method.
High-gain integrated in-line few-mode amplifier enabling 3840-km long-haul transmission
Authors: Xu Tao , Gao Tianyu , Wang Yanze , Li Wenhao , Li Wei , Du Cheng , Yang Zhiqun , Liu Yaping , Zhang Lin
Published: October 2022

An integrated few-mode erbium-doped fiber amplifier (FM-EDFA) with high modal gain is suitable for the in-line amplification in mode-division multiplexing transmission (MDM) systems. We first experimentally demonstrate a dual-stage integrated FM-EDFA supporting three linear polarization modes. Consisting of integrated passive components with low insertion losses, the FM-EDFA has a similar structure and performance to widely used commercial single-mode EDFAs. The averaged modal gain of 25 dB, the differential modal gain (DMG) of

<
1.1
  
dB

, and noise figures of 5–7 dB are simultaneously achieved. In addition, the DMG of the 3M-EDF itself is

∼
0.3
  
dB

. Moreover, an MDM transmission experiment with the in-line few-mode amplification by our proposed FM-EDFA over a 3840-km few-mode fiber link for a 28-Gbaud quadrature phase-shift keying (QPSK) signal is demonstrated.
Nascent polypeptide-Associated Complex and Signal Recognition Particle have cardiac-specific roles in heart development and remodeling
Authors: Schroeder Analyne M. , Nielsen Tanja , Lynott Michaela , Vogler Georg , Colas Alexandre R. , Bodmer Rolf
Published: October 2022

Establishing a catalog of Congenital Heart Disease (CHD) genes and identifying functional networks would improve our understanding of its oligogenic underpinnings. Our studies identified protein biogenesis cofactors Nascent polypeptide-Associated Complex (NAC) and Signal-Recognition-Particle (SRP) as disease candidates and novel regulators of cardiac differentiation and morphogenesis. Knockdown (KD) of the alpha- (Nacα) or beta-subunit (bicaudal, bic) of NAC in the developing Drosophila heart disrupted cardiac developmental remodeling resulting in a fly with no heart. Heart loss was rescued by combined KD of Nacα with the posterior patterning Hox gene Abd-B. Consistent with a central role for this interaction in cardiogenesis, KD of Nacα in cardiac progenitors derived from human iPSCs impaired cardiac differentiation while co-KD with human HOXC12 and HOXD12 rescued this phenotype. Our data suggest that Nacα KD preprograms cardioblasts in the embryo for abortive remodeling later during metamorphosis, as Nacα KD during translation-intensive larval growth or pupal remodeling only causes moderate heart defects. KD of SRP subunits in the developing fly heart produced phenotypes that targeted specific segments and cell types, again suggesting cardiac-specific and spatially regulated activities. Together, we demonstrated directed function for NAC and SRP in heart development, and that regulation of NAC function depends on Hox genes.
Cross-species transmission of an ancient endogenous retrovirus and convergent co-option of its envelope gene in two mammalian orders
Authors: Simpson J’Zaria , Kozak Christine A. , Boso Guney
Published: October 2022

Endogenous retroviruses (ERVs) found in vertebrate genomes are remnants of retroviral invasions of their ancestral species. ERVs thus represent molecular fossil records of ancient retroviruses and provide a unique opportunity to study viral-host interactions, including cross-species transmissions, in deep time. While most ERVs contain the mutated remains of the original retrovirus, on rare occasions evolutionary selection pressures lead to the co-option/exaptation of ERV genes for a host function. Here, we report the identification of two ancient related non-orthologous ERVenvgenes,ARTenvVandCARenvV, that are preserved with large open reading frames (ORFs) in the mammalian orders Artiodactyla and Carnivora, respectively, but are not found in other mammals. These Env proteins lack a transmembrane motif, but phylogenetic analyses show strong sequence preservation and positive selection of theenvsurface ORF in their respective orders, and transcriptomic analyses show a broad tissue expression pattern for bothARTenvVandCARenvV, suggesting that these genes may be exapted for a host function. Multiple lines of evidence indicate thatARTenvVandCARenvVwere derived from an ancient ancestral exogenous gamma-like retrovirus that was independently endogenized in two mammalian orders more than 60 million years ago, which roughly coincides with the K-Pg mass extinction event and subsequent mammalian diversification. Thus, these findings identify the oldest known retroviral cross-ordinal transmission of a gamma-like retrovirus with no known extant infectious counterpart in mammals, and the first discovery of the convergent co-option of an ERV gene derived from the same ancestral retrovirus in two different mammalian orders.
Demographic and professional risk factors of SARS-CoV-2 infections among physicians in low- and middle-income settings: Findings from a representative survey in two Brazilian states
Authors: Russo Giuliano , Cassenote Alex , De Oliveira Bruno Luciano Carneiro Alves , Scheffer Mário
Published: October 2022

Health workers (HWs) are a key resource for health systems worldwide, and have been affected heavily by the COVID-19 pandemic. Evidence is consolidating on incidence and drivers of infections, predominantly in high-income settings. It is however unclear what the risk factors may be for specific health professions, particularly in low- and middle-income countries (LMICs). We conducted a cross-sectional survey in a representative sample of 1,183 medical doctors registered with Brazil’s Federal Council of Medicine in one developed (São Paulo) and one disadvantaged state (Maranhão). Between February-June 2021, we administered a telephone questionnaire to collect data on physicians’ demographics, deployment to services, vaccination status, and self-reported COVID-19 infections. We performed descriptive, univariate, and multilevel clustered analysis to explore the association between physicians’ infection rates, and their sociodemographic and employment characteristics. A generalized linear mixed model with a binomial distribution was used to estimate the adjusted odds ratio. We found that 35.8% of physicians in our sample declared having been infected with SARS-CoV-2 virus during the first year of the pandemic. The infection rate in Maranhão (49.2%) [95% CI 45.0–53.4] was almost twice that in São Paulo (24.1%) [95% CI 20.8–27.5]. Being a physician in Maranhão [95% CI 2.08–3.57], younger than 50 years [95% CI 1.41–2.89] and having worked in a COVID-19 ward [95% CI 1.28–2.27], were positively associated with the probability of infection. Conversely, working with diagnostic services [95% CI 0.53–0.96], in administrative functions [95% CI 0.42–0.80], or in teaching and research [95% CI 0.48–0.91] were negatively associated. Based on our data from Brazil, COVID-19 infections in LMICs may be more likely in health systems with lower physician-to-patient ratios, and younger doctors working in COVID-19 wards may be infected more frequently. Such findings may be used to identify policies to mitigate COVID-19 effects on HWs in LMICs.
Improving the quality of HIV rapid testing in Ghana using the dried tube specimen-based proficiency testing program
Authors: Nkrumah Bernard , Iriemenam Nnaemeka C. , Frimpong Francis , Kalou Mireille B. , Botchway Berenice , Adukpo Rowland , Jackson Keisha G. , Angra Pawan , Whistler Toni , Adhikari Amitabh P. , Ayisi-Addo Stephen , Melchior Michael A.
Published: October 2022

Background

The introduction of human immunodeficiency virus (HIV) antibody rapid testing (RT) in resource-limited settings has proven to be a successful intervention to increase access to prevention measures and improve timely linkage to care. However, the quality of testing has not always kept pace with the scale-up of this testing strategy. To monitor the accuracy of HIV RT test results, a national proficiency testing (PT) program was rolled out at selected testing sites in Ghana using the dried tube specimen (DTS) approach.

Methods

Between 2015 and 2018, 635 HIV testing sites, located in five regions and supported by the U.S. President’s Emergency Plan for AIDS Relief (PEPFAR), were enrolled in the HIV PT program of the Ghana Health Service National AIDS/STI Control Programme. These sites offered various services: HIV Testing and Counselling (HTC), prevention of mother-to-child transmission (PMTCT) and Antiretroviral Treatment (ART). The PT panels, composed of six DTS, were prepared by two regional laboratories, using fully characterized plasma obtained from the regional blood banks and distributed to the testing sites. The results were scored by the PT providers according to the predefined acceptable performance criteria which was set at ≥ 95%.

Results

Seven rounds of PT panels were completed successfully over three years. The number of sites enrolled increased from 205 in round 1 (June 2015) to 635 in round 7 (December 2018), with a noticeable increase in Greater Accra and Eastern regions. The average participation rates of enrolled sites ranged from 88.0% to 98.0% across the PT rounds. By round 7, HTC (257/635 (40.5%)) and PMTCT (237/635 (37.3%)) had a larger number of sites that participated in the PT program than laboratory (106/635 (16.7%)) and ART (12/635 (1.9%)) sites. The average testing performance rate improved significantly from 27% in round 1 to 80% in round 7 (p < 0.001). The highest performance rate was observed for ART (100%), HTC (92%), ANC/PMTCT (90%) and Laboratory (89%) in round 5.

Conclusion

The DTS PT program showed a significant increase in the participation and performance rates during this period. Sub-optimal performances observed was attributed to non-compliance to the national testing algorithm and testing technique. However, the implementation of review meetings, peer-initiated corrective action, supportive supervisory training, and mentorship proved impactful. The decentralized approach to preparing the PT panels ensured ownership by the region and districts.
Sero-surveillance for IgG to SARS-CoV-2 at antenatal care clinics in three Kenyan referral hospitals: Repeated cross-sectional surveys 2020–21
Authors: Lucinde Ruth K. , Mugo Daisy , Bottomley Christian , Karani Angela , Gardiner Elizabeth , Aziza Rabia , Gitonga John N. , Karanja Henry , Nyagwange James , Tuju James , Wanjiku Perpetual , Nzomo Edward , Kamuri Evans , Thuranira Kaugiria , Agunda Sarah , Nyutu Gideon , Etyang Anthony O. , Adetifa Ifedayo M. O. , Kagucia Eunice , Uyoga Sophie , Otiende Mark , Otieno Edward , Ndwiga Leonard , Agoti Charles N. , Aman Rashid A. , Mwangangi Mercy , Amoth Patrick , Kasera Kadondi , Nyaguara Amek , Ng’ang’a Wangari , Ochola Lucy B. , Namdala Emukule , Gaunya Oscar , Okuku Rosemary , Barasa Edwine , Bejon Philip , Tsofa Benjamin , Ochola-Oyier L. Isabella , Warimwe George M. , Agweyu Ambrose , Scott J. Anthony G. , Gallagher Katherine E.
Published: October 2022

Introduction

The high proportion of SARS-CoV-2 infections that have remained undetected presents a challenge to tracking the progress of the pandemic and estimating the extent of population immunity.

Methods

We used residual blood samples from women attending antenatal care services at three hospitals in Kenya between August 2020 and October 2021and a validated IgG ELISA for SARS-Cov-2 spike protein and adjusted the results for assay sensitivity and specificity. We fitted a two-component mixture model as an alternative to the threshold analysis to estimate of the proportion of individuals with past SARS-CoV-2 infection.

Results

We estimated seroprevalence in 2,981 women; 706 in Nairobi, 567 in Busia and 1,708 in Kilifi. By October 2021, 13% of participants were vaccinated (at least one dose) in Nairobi, 2% in Busia. Adjusted seroprevalence rose in all sites; from 50% (95%CI 42–58) in August 2020, to 85% (95%CI 78–92) in October 2021 in Nairobi; from 31% (95%CI 25–37) in May 2021 to 71% (95%CI 64–77) in October 2021 in Busia; and from 1% (95% CI 0–3) in September 2020 to 63% (95% CI 56–69) in October 2021 in Kilifi. Mixture modelling, suggests adjusted cross-sectional prevalence estimates are underestimates; seroprevalence in October 2021 could be 74% in Busia and 72% in Kilifi.

Conclusions

There has been substantial, unobserved transmission of SARS-CoV-2 in Nairobi, Busia and Kilifi Counties. Due to the length of time since the beginning of the pandemic, repeated cross-sectional surveys are now difficult to interpret without the use of models to account for antibody waning.
Altered brain responses to noxious dentoalveolar stimuli in high-impact temporomandibular disorder pain patients
Authors: Peck Connor M. , Bereiter David A. , Eberly Lynn E. , Lenglet Christophe , Moana-Filho Estephan J.
Published: October 2022

High-impact temporomandibular disorder (TMD) pain may involve brain mechanisms related to maladaptive central pain modulation. We investigated brain responses to stimulation of trigeminal sites not typically associated with TMD pain by applying noxious dentoalveolar pressure to high- and low-impact TMD pain cases and pain-free controls during functional magnetic resonance imaging (fMRI). Fifty female participants were recruited and assigned to one of three groups based on the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) and Graded Chronic Pain Scale: controls (n = 17), low-impact (n = 17) and high-impact TMD (n = 16). Multimodal whole-brain MRI was acquired following the Human Connectome Project Lifespan protocol, including stimulus-evoked fMRI scans during which painful dentoalveolar pressure was applied to the buccal gingiva of participants. Group analyses were performed using non-parametric permutation tests for parcellated cortical and subcortical neuroimaging data. There were no significant between-group differences for brain activations/deactivations evoked by the noxious dentoalveolar pressure. For individual group mean activations/deactivations, a gradient in the number of parcels surviving thresholding was found according to the TMD pain grade, with the highest number seen in the high-impact group. Among the brain regions activated in chronic TMD pain groups were those previously implicated in sensory-discriminative and motivational-affective pain processing. These results suggest that dentoalveolar pressure pain evokes abnormal brain responses to sensory processing of noxious stimuli in high-impact TMD pain participants, which supports the presence of maladaptive brain plasticity in chronic TMD pain.
Adherence to multidisciplinary care in a prospective chronic kidney disease cohort is associated with better outcomes
Authors: Rios Pablo , Sola Laura , Ferreiro Alejandro , Silvariño Ricardo , Lamadrid Verónica , Ceretta Laura , Gadola Liliana ,   on behalf of all nephrologists who report to Programa de Salud Renal Uruguay Registry
Published: October 2022

Introduction

The Renal Healthcare Program Uruguay (NRHP-UY) is a national, multidisciplinary program that provides care to chronic kidney disease (CKD) patients. In this study, we report the global results of CKD patient outcomes and a comparison between those treated at the NRHP-UY Units, with those patients who were initially included in the program but did not adhere to follow up.

Methods

A cohort of not-on dialysis CKD patients included prospectively in the NRHP-UY between October 1st 2004 and September 30th 2017 was followed-up until September 30th 2019. Two groups were compared: a) Nephrocare Group: Patients who had at least one clinic visit during the first year on NRHP-UY (n = 11174) and b) Non-adherent Group: Patients who were informed and accepted to be included but had no subsequent data registered after admission (n = 3485). The study was approved by the Ethics Committee and all patients signed an informed consent. Outcomes were studied with Logistic and Cox´s regression analysis, Fine and Gray competitive risk and propensity-score matching tests.

Results

14659 patients were analyzed, median age 70 (60–77) years, 56.9% male. The Nephrocare Group showed improved achievement of therapeutic goals, ESKD was more frequent (HR 2.081, CI 95%1.722–2.514) as planned kidney replacement therapy (KRT) start (OR 2.494, CI95% 1.591–3.910), but mortality and the combined event (death and ESKD) were less frequent (HR 0.671, CI95% 0.628–0.717 and 0.777, CI95% 0.731–0.827) (p = 0.000) compared to the Non-adherent group. Results were similar in the propensity-matched group: ESKD (HR 2.041, CI95% 1.643–2.534); planned kidney replacement therapy (KRT) start (OR 2.191, CI95% 1.322–3.631) death (HR 0.692, CI95% 0.637–0.753); combined event (HR 0.801, CI95% 0.742–0.865) (p = 0.000).

Conclusion

Multidisciplinary care within the NRHP-UY is associated with timely initiation of KRT and lower mortality in single outcomes, combined analysis, and propensity-matched analysis.
A measurement invariance analysis of selected Opioid Overdose Knowledge Scale (OOKS) items among bystanders and first responders
Authors: Swartz James A. , Lin Qiao , Kim Yerim
Published: October 2022

The Opioid Overdose Knowledge Scale (OOKS) is widely used as an adjunct to opioid education and naloxone distribution (OEND) for assessing pre- and post-training knowledge. However, the extent to which the OOKS performs comparably for bystander and first responder groups has not been well determined. We used exploratory structural equation modeling (ESEM) to assess the measurement invariance of an OOKS item subset when used as an OEND training pre-test. We used secondary analysis of pre-test data collected from 446 first responders and 1,349 bystanders (N = 1,795) attending OEND trainings conducted by two county public health departments. Twenty-four items were selected by practitioner/trainer consensus from the original 45-item OOKS instrument with an additional 2 removed owing to low response variation. We used exploratory factor analysis (EFA) followed by ESEM to identify a factor structure, which we assessed for configural, metric, and scalar measurement invariance by participant group using the 22 dichotomous items (correct/incorrect) as factor indicators. EFA identified a 3-factor model consisting of items assessing: basic overdose risk information, signs of an overdose, and rescue procedures/advanced overdose risk information. Model fit by ESEM estimation versus confirmatory factor analysis showed the ESEM model afforded a better fit. Measurement invariance analyses indicated the 3-factor model fit the data across all levels of invariance per standard fit statistic metrics. The reduced set of 22 OOKS items appears to offer comparable measurement of pre-training knowledge on opioid overdose risks, signs of an overdose, and rescue procedures for both bystanders and first responders.
Deploying deep learning models on unseen medical imaging using adversarial domain adaptation
Authors: Valliani Aly A. , Gulamali Faris F. , Kwon Young Joon , Martini Michael L. , Wang Chiatse , Kondziolka Douglas , Chen Viola J. , Wang Weichung , Costa Anthony B. , Oermann Eric K.
Published: October 2022

The fundamental challenge in machine learning is ensuring that trained models generalize well to unseen data. We developed a general technique for ameliorating the effect of dataset shift using generative adversarial networks (GANs) on a dataset of 149,298 handwritten digits and dataset of 868,549 chest radiographs obtained from four academic medical centers. Efficacy was assessed by comparing area under the curve (AUC) pre- and post-adaptation. On the digit recognition task, the baseline CNN achieved an average internal test AUC of 99.87% (95% CI, 99.87-99.87%), which decreased to an average external test AUC of 91.85% (95% CI, 91.82-91.88%), with an average salvage of 35% from baseline upon adaptation. On the lung pathology classification task, the baseline CNN achieved an average internal test AUC of 78.07% (95% CI, 77.97-78.17%) and an average external test AUC of 71.43% (95% CI, 71.32-71.60%), with a salvage of 25% from baseline upon adaptation. Adversarial domain adaptation leads to improved model performance on radiographic data derived from multiple out-of-sample healthcare populations. This work can be applied to other medical imaging domains to help shape the deployment toolkit of machine learning in medicine.
Aspergillus violaceofuscus alleviates cadmium and chromium stress in Okra through biochemical modulation
Authors: Aziz Laila , Hamayun Muhammad , Rauf Mamoona , Iqbal Amjad , Husssin Anwar , Khan Sumera Afzal , Shafique Maryam , Arif Muhammad , Ahmad Ayaz , Rehman Gauhar , Ali Sajid , Kang Sang Mo , Lee In-Jung
Published: October 2022

Endophytic fungi from the Chilli were used to help okra plants exposed to cadmium (Cd) or chromium (Cr) stress. Initially, the strain Ch06 produced higher amounts of indole acetic acid (IAA) (230.5 μg/mL), sugar (130.7 μg/mL), proteins (128.2 μg/mL), phenolics (525.6 μg/mL) and flavonoids (98.4 μg/mL) in Czapek broth supplemented with Cd or Cr. The production of IAA and other metabolites in such a higher concentration suggested that Ch06 might improve plant growth under heavy metal stress. For this reason, an experiment was designed, in which biomass of Ch06 (at 2g/100g of sand) were applied to the okra plants exposed to Cd or Cr stress (at 100 or 500 μg/g). The results exhibited that Ch06 improved the total chlorophyll (36.4±0.2 SPAD), shoot length (22.6±0.2 cm), root length (9.1±0.6 cm), fresh weight (5±0.6 g), dry weight (1.25±0.01 g), sugars (151.6 μg/g), proteins (114.8 μg/g), proline (6.7 μg/g), flavonoids (37.9 μg/g), phenolics (70.7 μg/g), IAA (106.7 μg/g), catalase (0.75 enzyme units/g tissue) and ascorbic acid oxidaze (2.2 enzyme units/g tissue) of the associated okra plants. Similar observations have been recorded in Ch06 associated okra plants under Cd and Cr stress. Also, Ch06 association reduced translocation of Cd (35% and 45%) and Cr (47% and 53%) to the upper parts of the okra plants and thus reduced their toxicity. The internal transcribed spacer (ITS) region amplification of 18S rDNA (ribosomal deoxyribo nucleic acid) exhibited that the potent strain Ch06 was Aspergillus violaceofuscus. The results implied that A. violaceofuscus has the ability to promote host species growth exposed to Cd and Cr. Moreover, it helped the host plants to recover in Cd and Cr polluted soils, hence can be used as biofertilizer.
Mortality among mine and mill workers exposed to respirable crystalline silica
Authors: Kleinschmidt Sarah E. , Andres Kara L. , Holen Brian M. , Buehrer Betsy D. , Durand Gerardo , Taiwo Oyebode , Olsen Geary W.
Published: October 2022

Background

Millions of workers are potentially exposed to respirable crystalline silica (RCS) which has been associated with several diseases. We updated the mortality experience of a cohort of 2,650 mine and mill workers at four manufacturing facilities to assess cause-specific mortality risks associated with estimated cumulative RCS exposure.

Methods

Study eligibility was defined as any employee who had ≥1 year of service by 2000, with work history experience available from 1945 through 2004. Vital status and cause of death were ascertained from 1945 through 2015. RCS exposure was estimated across plant-, department-, job-, and time-dependent categories using historic industrial hygiene sampling data and professional judgment. Associations between cumulative RCS (mg/m3-years) and cause-specific mortality were examined using Cox proportional hazard regression models.

Results

In the exposure-response analysis defined on quartiles of cumulative RCS exposure, no increasing trend (ptrend = 0.37) in lung cancer mortality (n = 116 deaths) was observed (Hazard ratio (HR) = 1.00 (referent), 1.20, 1.85, 0.92). Mortality risk for non-malignant respiratory disease was increased across quartiles (HR = 1.00, 1.35, 1.89, 1.70; ptrend = 0.15), based on 83 deaths. Non-malignant renal disease mortality was increased across quartiles (HR = 1.00, 6.64, 3.79, 3.29; ptrend = 0.11), based on 26 deaths.

Conclusions

After nearly seven decades of follow-up, the exposure-response analyses showed no evidence of a positive trend for lung cancer, and limited evidence of a trend for non-malignant respiratory disease, and non-malignant renal disease mortality as a result of cumulative RCS exposure in this occupational cohort.
Depletion of Scleraxis-lineage cells during tendon healing transiently impairs multi-scale restoration of tendon structure during early healing
Authors: Korcari Antonion , Muscat Samantha , McGinn Elizabeth , Buckley Mark R. , Loiselle Alayna E.
Published: October 2022

Tendons are composed of a heterogeneous cell environment, with Scleraxis-lineage (ScxLin) cells being the predominant population. Although ScxLin cells are required for maintenance of tendon homeostasis, their functions during tendon healing are unknown. To this end, we first characterized the spatiotemporal dynamics of ScxLin cells during tendon healing, and identified that the overall ScxLin pool continuously expands up to early remodeling healing phase. To better define the function of ScxLin cells during the late proliferative phase of healing, we inducibly depleted ScxLin cells from day 14–18 post-surgery using the Scx-Cre; Rosa-DTR mouse model, with local administration of diphtheria toxin inducing apoptosis of ScxLin cells in the healing tendon. At D28 post-surgery, ScxLin cell depleted tendons (DTRScxLin) had substantial impairments in structure and function, relative to WT, demonstrating the importance of ScxLin cells during tendon healing. Next, bulk RNAseq was utilized to identify the underlying mechanisms that were impaired with depletion and revealed that ScxLin depletion induced molecular and morphological stagnation of the healing process at D28. However, this stagnation was transient, such that by D56 tendon mechanics in DTRScxLin were not significantly different than wildtype repairs. Collectively, these data offer fundamental knowledge on the dynamics and roles of ScxLin cells during tendon healing.
A genome resource for Acacia, Australia’s largest plant genus
Authors: McLay Todd G. B. , Murphy Daniel J. , Holmes Gareth D. , Mathews Sarah , Brown Gillian K. , Cantrill David J. , Udovicic Frank , Allnutt Theodore R. , Jackson Chris J.
Published: October 2022

Acacia(Leguminosae, Caesalpinioideae, mimosoid clade) is the largest and most widespread genus of plants in the Australian flora, occupying and dominating a diverse range of environments, with an equally diverse range of forms. For a genus of its size and importance,Acaciacurrently has surprisingly few genomic resources.Acacia pycnantha, the golden wattle, is a woody shrub or tree occurring in south-eastern Australia and is the country’s floral emblem. To assemble a genome forA.pycnantha, we generated long-read sequences using Oxford Nanopore Technology, 10x Genomics Chromium linked reads, and short-read Illumina sequences, and produced an assembly spanning 814 Mb, with a scaffold N50 of 2.8 Mb, and 98.3% of complete Embryophyta BUSCOs. Genome annotation predicted 47,624 protein-coding genes, with 62.3% of the genome predicted to comprise transposable elements. Evolutionary analyses indicated a shared genome duplication event in the Caesalpinioideae, and conflict in the relationships betweenCercis(subfamily Cercidoideae) and subfamilies Caesalpinioideae and Papilionoideae (pea-flowered legumes). Comparative genomics identified a suite of expanded and contracted gene families inA.pycnantha, and these were annotated with both GO terms and KEGG functional categories. One expanded gene family of particular interest is involved in flowering time and may be associated with the characteristic synchronous flowering ofAcacia. This genome assembly and annotation will be a valuable resource for all studies involvingAcacia, including the evolution, conservation, breeding, invasiveness, and physiology of the genus, and for comparative studies of legumes.
Late Quaternary activity of the Xiadian west concealed fault east to Beijing and its tectonic significance
Authors: Shen Jun , Dai Xunye , Jiao Xuankai , Shao Bo
Published: October 2022

Beijing plain is a strong earthquake tectonic area in China. There was a Sanhe-Pinggu earthquake with Ms8 that happened in1679. The seismogenic fault of this earthquake is called Xiadian fault. Our work found fault with a similar strike and opposite dip in the west of the Xiadian fault, which is called the Xiadian west fault in this paper. Six shallow seismic profiles have been constructed to determine the location of the fault in Sanhe city, and the late Quaternary activity of the fault is studied with the method of combined drilling, magnetic susceptibility logging, and luminescence dating. The results of shallow seismic exploration show that the fault is zigzag and generally strikes NE and inclines NW. According to the core histogram and logging curves of ten boreholes and eight effective dating data, the buried depth of the upper breakpoint of the concealed fault is about 12 m, which dislocates the late Pleistocene strata. The effective dating result of this set of strata is 36.52 ±5.39 ka. The vertical slip rate has been about 0.075± 0.023 mm/a since the late Pleistocene and about 0.058 ± 0.030 mm/a since the late period of the late Pleistocene. It can be inferred that the Xiadian west fault is probably a part of the seismogenic structure of the Sanhe-Pinggu Ms8 earthquake that happened in 1679. In a broad sense, the Xiadian fault zone is likely to extend to the southwest along the Xiadian west fault.
Impact of the COVID-19 pandemic on community-dwelling older adults: A longitudinal qualitative study of participants from the Hertfordshire Cohort Study
Authors: Bloom Ilse , Zhang Jean , Hammond Julia , Bevilacqua Gregorio , Lawrence Wendy , Ward Kate A. , Cooper Cyrus , Dennison Elaine M.
Published: October 2022

Background

Older adults have been especially vulnerable to adverse effects from the COVID-19 pandemic including higher mortality and more severe disease complications. At the same time, social isolation, malnutrition and physical inactivity are serious concerns among older adults. The pandemic and associated restrictions may serve to exacerbate these issues, presenting increased risks to physical and mental health. The aims of this qualitative study were: i) to explore how community-living older people in the UK experienced the first wave of the COVID-19 pandemic, specifically how it impacted their well-being and associated health behaviours; ii) to explore how older people’s experiences and behaviours changed over time throughout the first wave.

Methods

Qualitative data were collected by conducting serial telephone interviews, with an interval of approximately three months. Participants were from the Hertfordshire Cohort Study, all aged over 80 years. Discussions were audio-recorded, information related to the COVID-19 pandemic was transcribed verbatim and transcripts analysed thematically. Interviews were conducted from March to October 2020.

Results

Data for twelve participants (7 men and 5 women) from a total of 35 interviews were used, comprising two or three timepoints per participant. Analysis identified five overarching themes: 1) shopping strategies and food accessibility, 2) limitations on activities and going out, 3) disruption to healthcare, 4) social and psychological repercussions, and 5) coping strategies. Findings highlight challenges associated with accessing shops, healthcare, and usual activities due to pandemic-related restrictions. Longitudinal findings showed that for some, the ongoing pandemic and related restrictions appeared to aggravate mental health issues (low mood, anxiety) over time, as well as greater feelings of isolation or loneliness, reduced activity and functional limitations; this was despite some relaxation of restrictions later on. Coping strategies used by participants included finding ways to keep busy and to do physical activity safely, maintaining social contact remotely, and having an optimistic or positive outlook, a ‘do what you can’ attitude.

Conclusions

Interventions are likely to be needed in the wake of the COVID-19 pandemic to support health behaviours, such as increasing physical activity, social engagement and improving mental health among community-living older adults.
Wound healing in db/db mice with type 2 diabetes using non-contact exposure with an argon non-thermal atmospheric pressure plasma jet device
Authors: Iswara Arya , Tanaka Kenta , Ishijima Tatsuo , Nakajima Yukari , Mukai Kanae , Tanaka Yasunori , Nakano Yusuke , Sugama Junko , Oe Makoto , Okuwa Mayumi , Nakatani Toshio
Published: October 2022

A non-thermal atmospheric pressure plasma jet (APPJ) may stimulate cells and tissues or result in cell death depending on the intensity of plasma at the target; therefore, we herein investigated the effects of non-thermal plasma under non-contact conditions on the healing of full-thickness wounds in diabetic mice (DM+ group) and normal mice (DM- group). A hydrogen peroxide colorimetric method and high performance liquid chromatography showed that APPJ produced low amounts of reactive oxygen and nitrogen species. Ten-week-old male C57BL/6j mice with normal blood glucose levels (DM- group) and 10-week-old male C57BLKS/J Iar-+Leprdb/+Leprdb mice (DM+ group) received two full-thickness cutaneous wounds (4 mm in diameter) on both sides of the dorsum. Wounds were treated with or without the plasma jet or argon gas for 1 minute and were then covered with a hydrocolloid dressing (Hydrocolloid), according to which mice were divided into the following groups: DM+Plasma, DM+Argon, DM+Hydrocolloid, DM-Plasma, DM-Argon, and DM-Hydrocolloid. Exudate weights, wound areas, and wound area ratios were recorded every day. Hematoxylin and eosin staining was performed to assess re-epithelialization and α-SMA immunohistological staining to evaluate the formation of new blood vessels. Non-thermal plasma under non-contact conditions reduced the production of exudate. Exudate weights were smaller in the DM+Plasma group than in the DM+Hydrocolloid and DM+Argon groups. The wound area ratio was smaller for plasma-treated wounds, and was also smaller in the DM+Plasma group than in the DM+Hydrocolloid and DM+Argon groups on days 1–21 (p<0.01). Wound areas were smaller in the DM-Plasma group than in the DM-Argon group until day 14 and differences were significant on days 1–5 (p<0.01). The percentage of re-epithelialization was significantly higher in the DM+Plasma group than in the DM+Argon and DM+Hydrocolloid groups (p<0.01). The number of new blood vessels that had formed by day 7 was significantly higher in the DM+Plasma group than in the DM+Hydrocolloid and DM+Argon groups (p<0.05). These results indicate that treatment with the current non-thermal plasma APPJ device under non-contact conditions accelerated wound healing in diabetic mice.
Understanding factors that could influence patient acceptability of the use of the PINCER intervention in primary care: A qualitative exploration using the Theoretical Framework of Acceptability
Authors: Laing Libby , Salema Nde-eshimuni , Jeffries Mark , Shamsuddin Azwa , Sheikh Aziz , Chuter Antony , Waring Justin , Avery Anthony , Keers Richard N.
Published: October 2022

Introduction

Medication errors are an important cause of morbidity and mortality. The pharmacist-led IT-based intervention to reduce clinically important medication errors (PINCER) intervention was shown to reduce medication errors when tested in a cluster randomised controlled trial and when implemented across one region of England. Now that it has been rolled out nationally, and to enhance findings from evaluations with staff and stakeholders, this paper is the first to report patients’ perceived acceptability on the use of PINCER in primary care and proposes suggestions on how delivery of PINCER related care could be delivered in a way that is acceptable and not unnecessarily burdensome.

Methods

A total of 46 participants living with long-term health conditions who had experience of medication reviews and/or monitoring were recruited through patient participant groups and social media. Semi-structured, qualitative interviews and focus groups were conducted face-to-face or via telephone. A thematic analysis was conducted and findings mapped to the constructs of the Theoretical Framework of Acceptability (TFA).

Results

Two themes were identified and interpreted within the most relevant TFA construct: Perceptions on the purpose and components of PINCER (Affective Attitude and Intervention Coherence) and Perceived patient implications (Burden and Self-efficacy). Overall perceptions on PINCER were positive with participants showing good understanding of the components. Access to medication reviews, which PINCER related care can involve, was reported to be limited and a lack of consistency in practitioners delivering reviews was considered challenging, as was lack of communication between primary care and other health-care providers. Patients thought it would be helpful if medication reviews and prescription renewal times were synchronised. Remote medication review consultations were more convenient for some but viewed as a barrier to communication by others. It was acknowledged that some patients may be more resistant to change and more willing to accept changes initiated by general practitioners.

Conclusions

Participants found the concept of PINCER acceptable; however, acceptability could be improved if awareness on the role of primary care pharmacists is raised and patient-pharmacist relationships enhanced. Being transparent with communication and delivering streamlined and consistent but flexible PINCER related care is recommended.
Commitment to protective measures during the COVID-19 pandemic in Syria: A nationwide cross-sectional study
Authors: Shibani Mosa , Alzabibi Mhd Amin , Mohandes Abdul Fattah , Armashi Humam , Alsuliman Tamim , Mouki Angie , Mansour Marah , Ismail Hlma , Alhayk Shahd , Rmman Ahmad abdulateef , Almohi Alsaid Mushaweh Hala Adel , Battikh Elias , Khalayli Naram , Sawaf Bisher , Kudsi Mayssoun
Published: October 2022

Background

Severe acute respiratory syndrome coronavirus 2 continues to impose itself on all populations of the world. Given the slow pace of vaccination in the developing world and the absence of effective treatments, adherence to precautionary infection control measures remains the best way to prevent the COVID-19 pandemic from spiraling out of control. In this study, we aim to evaluate the extent to which the Syrian population adheres to these measures and analyze the relationship between demographic variables and adherence.

Methods

This cross-sectional study took place in Syria between January 17 and March 17, 2021. A structured self-administered questionnaire was used to collect the data. The questionnaire was distributed in both electronic and printed versions. Our sample consisted of 7531 individuals. Collected data were analyzed using SPSS v.25. The chi-square test was used to address the correlation between adherence and demographic variables.

Results

Of the 10083 reached out, only 8083 responded, and 7531 included in the final analysis with an effective response rate of 74.7%. Of them, 4026 (53.5%) were women, 3984 (52.9%) were single, and 1908 (25.3%) had earned university degrees. 5286 (70.25) were in the high level of adherence category to protective measures. Statistically significant differences were documented when investigating the correlation between commitment to preventive measures and age, sex, marital status, financial status, employment, and educational attainment. Furthermore, those who believed that COVID-19 poses a major risk to them, or society were more committed to preventive measures than those who did not.

Conclusion

The participants in this study generally showed a high level of adherence to the preventive measures compared to participants in other studies from around the world, with some concerns regarding the sources of information they depend on. Nationwide awareness campaigns should be conducted and focus on maintaining, if not expanding, this level of commitment, which would mitigate the pandemic’s impact on Syrian society.
Does “lottery culture” affect household financial decisions? Evidence from China
Authors: Cao Dongmei , Wang Dan , Liao Yujia , Liu Qing
Published: October 2022

In recent years, China’s “lottery culture” has developed vigorously. Moreover, the investment participation rate of Chinese families in the formal financial market is low, whereas that in the informal financial market is high. Is there a certain relationship between “lottery culture” and family financial decision-making? If so, what is the underlying mechanism? Based on the 2017 CHFS data and lottery sales data of provinces, this study explores the impact of “lottery culture” on household participation in the formal and informal financial markets and the diversity of household financial portfolios. Results show that “lottery culture” can impede household participation in the formal financial market and the diversity of household financial portfolios while promoting household participation in the informal financial market in China. Furthermore, we analyze two channels of “lottery culture” impacts on household financial decisions: (1) risk attitude and (2) human capital. Results illustrate that “lottery culture” can influence household financial decisions by increasing risk tolerance and reducing the human capital of households.
Implementing quality improvement intervention to improve intrapartum fetal heart rate monitoring during COVID-19 pandemic- observational study
Authors: Bhattarai Pratiksha , Gurung Rejina , Basnet Omkar , Malla Honey , Målqvist Mats , K. C. Ashish
Published: October 2022

Introduction

Adherence to intrapartum fetal heart rate monitoring (FHRM) for early decision making in high-risk pregnancies remains a global health challenge. COVID-19 has led to disruption of routine intrapartum care in all income settings. This study aims to evaluate the implementation of quality improvement (QI) intervention to improve intrapartum FHRM and birth outcome before and during pandemic.

Method and materials

We conducted an observational study among 10,715 pregnant women in a hospital of Nepal, over 25 months. The hospital implemented QI intervention i.e facilitated plan-do-study-act (PDSA) meetings before and during pandemic. We assessed the change in intrapartum FHRM, timely action in high-risk deliveries and fetal outcomes before and during pandemic.

Results

The number of facilitated PDSA meetings increased from an average of one PDSA meeting every 2 months before pandemic to an average of one PDSA meeting per month during the pandemic. Monitoring and documentation of intrapartum FHRM at an interval of less than 30 minutes increased from 47% during pre-pandemic to 73.3% during the pandemic (p<0.0001). The median time interval from admission to abnormal heart rate detection decreased from 160 minutes to 70 minutes during the pandemic (p = 0.020). The median time interval from abnormal FHR detection to the time of delivery increased from 122 minutes to 177 minutes during the pandemic (p = 0.019). There was a rise in abnormal FHR detection during the time of admission (1.8% vs 4.7%; p<0.001) and NICU admissions (2.9% vs 6.5%; p<0.0001) during the pandemic.

Conclusion

Despite implementation of QI intervention during the pandemic, the constrains in human resource to manage high risk women has led to poorer neonatal outcome. Increasing human resources to manage high risk women will be key to timely action among high-risk women and prevent stillbirth.