bluuebunny/crossref_metadata_2025 huggingface dataset:
Factors Influencing Primary Care Providers’ Unneeded Lumbar Spine MRI Orders for Acute, Uncomplicated Low-Back Pain: a Qualitative Study
Authors: Nevedal Andrea L. , Lewis Eleanor T. , Wu Justina , Jacobs Josephine , Jarvik Jeffrey G. , Chou Roger , Barnett Paul G.
Published: February 2020

Background

Clinical practice guidelines suggest that magnetic resonance imaging of the lumbar spine (LS-MRI) is unneeded during the first 6 weeks of acute, uncomplicated low-back pain. Unneeded LS-MRIs do not improve patient outcomes, lead to unnecessary surgeries and procedures, and cost the US healthcare system about $300 million dollars per year. However, why primary care providers (PCPs) order unneeded LS-MRI for acute, uncomplicated low-back pain is poorly understood.

Objective

To characterize and explain the factors contributing to PCPs ordering unneeded LS-MRI for acute, uncomplicated low-back pain.

Design

Qualitative study using semi-structured interviews.

Participants

Veterans Affairs PCPs identified from administrative data as having high or low rates of guideline-concordant LS-MRI ordering in 2016.

Approach

Providers were interviewed about their use of LS-MRI for acute, uncomplicated low-back pain and factors contributing to their decision-making. Directed content analysis of transcripts was conducted to identify and compare environmental-, patient-, and provider-level factors contributing to unneeded LS-MRI.

Key Results

Fifty-five PCPs participated (8.6% response rate). Both low (n = 33) and high (n = 22) guideline-concordant providers reported that LS-MRIs were required for specialty care referrals, but they differed in how other environmental factors (stringency of radiology utilization review, management of patient travel burden, and time constraints) contributed to LS-MRI ordering patterns. Low- and high-guideline-concordant providers reported similar patient factors (beliefs in value of imaging and pressure on providers). However, provider groups differed in how provider-level factors (guideline familiarity and agreement, the extent to which they acquiesced to patients, and belief in the value of LS-MRI) contributed to LS-MRI ordering patterns.

Conclusions

Results describe how diverse environmental, patient, and provider factors contribute to unneeded LS-MRI for acute, uncomplicated low-back pain. Prior research using a single intervention to reduce unneeded LS-MRI has been ineffective. Results suggest that multifaceted de-implementation strategies may be required to reduce unneeded LS-MRI.
Outcomes and revision rates of primary vs. secondary reverse total shoulder arthroplasty for proximal humeral fractures
Authors: Katthagen J. Christoph , Hesse Elina , Lill Helmut , Schliemann Benedikt , Ellwein Alexander , Raschke Michael J. , Imrecke Julia
Published: February 2020

Background

Reverse total shoulder arthroplasty (RSA) is a standard treatment for proximal humeral fractures (PHF) and its sequelae. In this study we analyzed the clinical outcomes of primary vs. secondary RSA for displaced PHF in elderly patients.

Methods

We retrospectively reviewed 68 cases of primary or secondary RSA for displaced PHF. For 51 patients (28 primary RSA, 23 secondary RSA), a minimum 12-month follow-up with clinical and radiological assessment was available. Clinical assessment comprised joint active range of motion, DASH, Constant–Murley Score (CMS), Subjective Shoulder Value, and Visual Analog Scale for pain. Outcomes and complications of patients with primary RSA were compared with those of patients with secondary RSA.

Results

Follow-up data were available for 45 women and six men with a mean age of 73 years (range, 59–87) at the time of implantation. In 28 cases, primary RSA was performed; in 23 cases, RSA was performed as a revision procedure for fracture sequelae after failed plating, nailing, or hemiarthroplasty. The mean age- and gender-related CMS was 82.2 ± 34.2% (raw mean CMS: 46.8 ± 19.6 points). Among nine of the 51 patients with follow-up data (17.6%), ten complications occurred with six surgical revisions. Primary RSA (n = 28) resulted in better clinical shoulder function compared with secondary RSA (n = 23). Significantly more complications and revision surgeries were observed following secondary than primary RSA (p = 0.013).

Conclusion

In this study, primary RSA for displaced PHF in the elderly was associated with better clinical function and lower complication and revision rates than secondary RSA. Predictive parameters for failure of humeral head-preserving fracture fixation and anatomic hemiarthroplasty should be carefully evaluated. Primary RSA should be considered when surgical treatment of PHF is indicated in elderly patients.
Exploring the Experiences of Women Who Develop Restrictive Eating Behaviours After Bariatric Surgery
Authors: Watson Charlotte , Riazi Afsane , Ratcliffe Denise
Published: February 2020

Objective

This study aimed to explore the experiences of women who had developed excessively restrictive eating behaviours following bariatric surgery.

Method

Five female participants, who were at least nine months post-bariatric surgery and exhibiting restrictive eating behaviours, were recruited from Bariatric Surgery Psychology Services and asked to complete qualitative face-to-face semi-structured interviews. The data was analysed using interpretative phenomenological analysis (IPA).

Results

Three super-ordinate themes emerged: (1) experiences of weight stigma and weight history on self, (2) the impact of loose skin, (3) thoughts about food and disordered eating patterns. These captured the impact of past weight-related experiences—including weight stigma, intense fears of weight gain, negative cognitions about the self, the impact of excess skin, changes in the way the women thought about food and restrictive eating behaviours.

Discussion

This study is one of the first to specifically explore restrictive eating disorders after bariatric surgery using a qualitative approach. The findings of this study may offer helpful aspects for professionals to hold in mind when identifying individuals with problematic restrictive eating behaviours following bariatric surgery. Body contouring surgery, internalised weight bias and weight stigma are explored in relation to the post-bariatric surgery treatment pathway. The current diagnostic criterion for anorexia nervosa are discussed to highlight difficulties in diagnosing this presentation in the post-bariatric surgery population, where people can have BMIs over 25 kg/m2 but are severely restricting energy intake.
The laser beamline in SULF facility
Authors: Zhang Zongxin , Wu Fenxiang , Hu Jiabing , Yang Xiaojun , Gui Jiayan , Ji Penghua , Liu Xingyan , Wang Cheng , Liu Yanqi , Lu Xiaoming , Xu Yi , Leng Yuxin , Li Ruxin , Xu Zhizhan
Published: February 2020

In this paper, we report the recent progress on the

$1~\text{PW}/0.1~\text{Hz}$

laser beamline of Shanghai Superintense Ultrafast Laser Facility (SULF). The SULF-1 PW laser beamline is based on the double chirped pulse amplification (CPA) scheme, which can generate laser pulses of 50.8 J at 0.1 Hz after the final amplifier; the shot-to-shot energy fluctuation of the amplified pulse is as low as 1.2% (std). After compression, the pulse duration of 29.6 fs is achieved, which can support a maximal peak power of 1 PW. The contrast ratio at

$-80~\text{ps}$

before main pulse is measured to be

$2.5\times 10^{-11}$

. The focused peak intensity is improved by optimizing the angular dispersion in the grating compressor. The maximal focused peak intensity can reach

$2.7\times 10^{19}~\text{W}/\text{cm}^{2}$

even with an

$f/26.5$

off-axis parabolic mirror. The horizontal and vertical angular pointing fluctuations in 1 h are measured to be 1.89 and

$2.45~\unicode[STIX]{x03BC}\text{rad}$

, respectively. The moderate repetition rate and the good stability are desirable characteristics for laser–matter interactions. The SULF-1 PW laser beamline is now in the phase of commissioning, and preliminary experiments of particle acceleration and secondary radiation under 300–400 TW/0.1 Hz laser condition have been implemented. The progress on the experiments and the daily stable operation of the laser demonstrate the availability of the SULF-1 PW beamline.
Diverse and Dynamic Dietary Patterns in Early Colonial Cuba: New Insights from Multiple Isotope Analyses
Authors: Laffoon Jason E. , Valcárcel Rojas Roberto , Weston Darlene A. , Hoogland Menno L. P. , Davies Gareth R. , Hofman Corinne L.
Published: February 2020

The European conquest and colonization of the Caribbean precipitated massive changes in indigenous cultures and societies of the region. One of the earliest changes was the introduction of new plant and animal foods and culinary traditions. This study presents the first archaeological reconstruction of indigenous diets and foodways in the Caribbean spanning the historical divide of 1492. We use multiple isotope datasets to reconstruct these diets and investigate the potential relationships between dietary and mobility patterns at multiple scales. Dietary patterns are assessed by isotope analyses of different skeletal elements from the archaeological skeletal population of El Chorro de Maíta, Cuba. This approach integrates carbon and nitrogen isotope analyses of bone and dentine collagen with carbon and oxygen isotope analyses of bone and enamel apatite. The isotope results document extreme intrapopulation dietary heterogeneity but few systematic differences in diet between demographic/social groups. Comparisons with published isotope data from other precolonial and colonial period populations in the Caribbean indicate distinct dietary and subsistence practices at El Chorro de Maíta. The majority of the local population consumed more animal protein resources than other indigenous populations in the Caribbean, and their overall dietary patterns are more similar to colonial period enslaved populations than to indigenous ones.
Millennial-scale increase in winter precipitation in the southern Rocky Mountains during the Common Era
Authors: Parish Meredith C. , Calder W. John , Shuman Bryan N.
Published: February 2020

We employed the modern analog technique to quantitatively reconstruct temperature and precipitation over the past 2500 yr based on fossil pollen records from six high-elevation lakes in northern Colorado. Reconstructed annual temperatures for the study area did not deviate significantly from modern over the past 2500 yr despite hemispheric expressions of Medieval Climate Anomaly warmth and Little Ice Age cooling. Annual precipitation, however, shifted from lower than modern rates from 2500 to 1000 cal yr BP to higher than modern rates after 1000 cal yr BP, a greater than 100 mm increase in precipitation. Winter precipitation accounts for the majority of the change in annual precipitation, while summer precipitation rates did not change significantly over the past 2500 yr. The large change in winter precipitation rates from the first to second millennium of the Common Era is inferred from a shift in fossil pollen assemblages dominated by subalpine conifers, which have southern sites as modern analogs, to assemblages representing open subalpine vegetation with abundant Artemisia spp. (sagebrush), which have more northern modern analogs. The change helps to explain regional increases in lake levels and shifts in some isotopic and tree-ring data sets, highlighting the risk of large reductions in snowpack and water supplies in the Intermountain West.
Luminescence and Radiocarbon Dating of Mortars at Milano-Bicocca Laboratories
Authors: Panzeri Laura , Maspero Francesco , Galli Anna , Sibilia Emanuela , Martini Marco
Published: February 2020

This work shows the results of optically stimulated luminescence (OSL) and radiocarbon (14C) dating applied to mortars of historical structures in northern Italy. All the results are compared with archaeological evidence and thermoluminescence (TL) dating of bricks. The main issue for OSL mortar dating is that the quartz grains contained in the mortar may be only partially bleached, leading to an overestimation of the sample age. In order to identify the best protocol to apply, both multi-grain (MG) and single grain (SG) methods were used. The minimum age model (MAM) statistical approach was applied to refine their accuracy. However, the identification of the bleached grains is not always successful, indicating that further investigations are needed to develop suitable dating protocol. For the 14C technique, a crucial aspect is the selection of anthropogenic calcite. In this work the mortars were treated using a Cryosonic method to select anthropogenic calcite from raw material, and the obtained powder was sieved to select the finer fraction. Unfortunately, only in two cases an acceptable amount of sample could be obtained. All the fractions were dated via accelerator mass spectrometry (AMS), and the results compared with independently obtained dates. The results show that the execution of the dating analysis requires previous characterizations to assess the nature of the mortar components and avoid unusable fractions.
Aragonite Fraction Dating of Vermetids in the Context of Paleo Sea-Level Curves Reconstruction
Authors: Moreira Vinicius N , Macario Kita D , Guimarães Renato B , Dias Fábio F , Araujo Julia C , Jesus Perla , Douka Katerina
Published: February 2020

Identifying and tackling recrystallization is a critical factor in the reliable radiocarbon (14C) dating of carbonates, since exogenous carbon can be incorporated and thus mask the real age of the samples. Vermetids are among the most important bioindicators used for paleo sea-level reconstruction, and the accuracy of their chronology can significantly impact sea-level curves. Age differences larger than 1 14C kyr before and after acid etching, combined with X-ray diffraction (XRD) analysis that indicates a significant amount of calcite still remains in the shell, led us to apply the previously developed carbonate density separation protocol (CarDS). Using a solution of sodium polytungstate, with density of 2.80 g/cm3, we successfully separated different carbonate fractions for a set of 10 vermetid samples from the coast of Rio de Janeiro, southeast of Brazil. Each separation was verified by XRD analysis and the 14C concentrations of different fractions were compared. The results show that the calcite fraction in the studied vermetid samples varied from 12 to 63% and aragonite fraction ages are up to 2 14C kyr older than the raw samples, thus confirming the efficacy of CarDS in removing young carbonates and the importance of density separation to vermetids prior to accelerator mass spectrometry (AMS) dating.
Fed by Famine: The Hindu Mahasabha's politics of religion, caste, and relief in response to the Great Bengal Famine, 1943–1944
Authors: SARKAR ABHIJIT
Published: February 2020

This article demonstrates how the Great Bengal Famine of 1943–1944 and relief activism during it fed the politics of the Hindu right, a development that has not previously received much scholarly attention. Using hitherto unused primary sources, the article introduces a novel site to the study of communal politics, namely, the propagation of Hindu communalism through food distribution during a humanitarian crisis. It examines the caste and class bias in private relief and provides the first in-depth study of the multifaceted process whereby the Hindu Mahasabha used the famine for political purposes. The party portrayed Muslim food officials as ‘saboteurs’ in the food administration, alleged that the Muslim League government was ‘creating’ a new group of Muslim grain traders undermining the established Hindu traders, and publicized the government's failure to avert the famine to prove the economic ‘unviability’ of creating Pakistan. This article also explores counter-narratives, for example, that Hindu political leaders were deliberately impeding the food supply in the hope that starvation would compel Bengali Muslims to surrender their demand for Pakistan. The politics of religious conversion played out blatantly in famine-relief when the Mahasabha accused Muslim volunteers of converting starving Hindus to Islam in exchange for food, and demanded that Hindu and Muslim famine orphans should remain in Hindu and Muslim orphanages respectively. Finally, by dwelling on beef consumption by the army at the time of an acute shortage of dairy milk during the famine, the Mahasabha fanned communal tensions surrounding the orthodox Hindu taboo on cow slaughter.
Can Emergency Medical Services (EMS) Shorten the Time to Stroke Team Activation, Computed Tomography (CT), and the Time to Receiving Antithrombotic Therapy? A Prospective Cohort Study
Authors: Alabdali Abdullah , Yousif Sami , Alsaleem Abdullah , Aldhubayb Mazen , Aljerian Nawfal
Published: February 2020

Introduction:

Stroke is a major emergency that can cause a significant morbidity and mortality. Advancement in stroke management in recent years has allowed more patients to be diagnosed and treated by stroke teams; however, stroke is a time-sensitive emergency that requires a high level of coordination, particularly within the prehospital phase. This research is to determine whether patients received by Emergency Medical Services (EMS) at a tertiary health care facility had shorter stroke team activation, time to computed tomography (CT), or time to receive intravenous thrombolytics.

Methods:

This research is a prospective cohort study of adults with stroke symptoms who required stroke team activation at a tertiary medical facility. The study included all patients received from September 1, 2017 through August 31, 2018. The primary outcome was the time difference to stroke team activation between patients received by EMS compared to patients that arrived by a private method of transportation. The secondary outcomes were the difference in time to CT scan and the time to receive intravenous recombinant tissue plasminogen activator (rtPA).

Results:

There were 75 (34.1%) patients who had been received by EMS, while 145 (65.9%) patients arrived via private transportation method (private car or by a friend/family member). The mean time to stroke team activation, time to CT, and time to receive thrombolytic therapy for the EMS group were: 8.19 (95% CI, 6.97 - 9.41) minutes; 18 (95% CI, 15.9 - 20.1) minutes; and 13.1 (95% CI, 6.95 - 19.3) minutes, respectively. Those for the private car group, on the other hand, were: 16 (95% CI, 12.4 - 19.6) minutes; 23.39 (95% CI, 19.6 - 27.2) minutes; and nine (95% CI, 4.54 -13.5) minutes, respectively. There was a significantly shorter time to stroke team activation for patients arriving via EMS compared to private car (P ≤ .00), but no significant difference was found on time to CT (P = .259) or time to receive rtPA (P = .100).

Conclusion:

Emergency Medical Service transportation of stroke patients can significantly shorten the time to stroke team activation, leading to shorter triage and accelerated patient management. However, there was no statistical difference in time to CT or time to receive rtPA. Patients with stroke symptoms may benefit more from EMS transportation compared to private methods of transportation.
Study of Medical Demand-Supply Balance for the Nankai Trough Earthquake
Authors: Takada Yosuke , Otomo Yasuhiro
Published: February 2020

Introduction:

The Nankai Trough, which marks the boundary between the Eurasian and Philippine Sea plates, is forecasted to create a catastrophic earthquake and tsunami within 30 years. The Japanese government believes that the number of casualties would be huge. However, the exact number of severely injured (SI) people who would need emergency and intensive care has not been identified.

Objective:

This study, therefore, aimed to clarify the gap between medical supplies and forecasted demand.

Methods:

The official data estimating the number of injured people were collected, together with the number of intensive care unit (ICU) and high care unit (HCU) beds from each prefecture throughout Japan. The number of SI cases was recalculated based on official data. The number of hospital beds was then compared with the number of SI people.

Results:

The total number of hospitals in Japan is 8,493 with 893,970 beds, including 6,556 ICU and 5,248 HCU beds. When the Nankai Trough earthquake occurs, 187 of the 723 disaster base hospitals (DBHs) would be located in the areas with a seismic intensity of an upper six on the Japanese Seismic Intensity Scale (JSIS) of seven, and 79 DBHs would be located in the tsunami inundation area. The estimated total number of injured people would be 661,604, including 26,857 severe, 290,065 moderate, and 344,682 minor cases.

Conclusion:

Even if all ICU and HCU beds were available for severe patients, an additional 15,053 beds would be needed. If 80% of beds were used in non-disaster times, the available ICU and HCU beds would be only 2,361. The Cabinet Office of Japan (Chiyoda City, Tokyo, Japan) assumes that 60% of hospital beds would be unavailable in an area with an upper six on the JSIS. The number of ICU and HCU beds that would be usable during a disaster would thus further decrease. The beds needed for severe patients, therefore, would be significantly lacking when the Nankai Trough earthquake occurs. It would be necessary to start the treatment of those severe patients who are “more likely to be saved.”
The Sun Herald Sydney City-2-Surf Fun Run – Historical Injury Patterns and Factors Influencing Injury Type and Frequency
Authors: Vassil John C. , Winn Linda , Heslop David J.
Published: February 2020

Introduction:

The Sydney City-2-Surf (Australia) fun run is the world’s largest annual run entered by around 80,000 people. First aid planning at mass-participation running events such as the City-2-Surf is an area in the medical literature that has received little attention. Consequently, first aid planning for these events is based on experience rather than evidence. The models for predicting casualties that currently exist in the literature are either dated or not statistically significant.

Aim:

The aim of this study was to characterize patterns of injuries linked to geographic location across the course of the City-2-Surf, and to explore relationships of injury types with location and meteorological conditions.

Methods:

Records for formally treated casualties and meteorological conditions were obtained for the race years 2010-2016 and statistically analyzed to find associations between meteorological conditions, geographic conditions, casualty types, and location.

Results:

The most common casualties encountered were heat exhaustion or hyperthermia (39.2%), musculoskeletal (25.4%), and physical exhaustion (10.2%). Associations were found between gradient and the location. Type of casualty incidence with the individual distribution trends of casualty types were quite clear. Clusters of musculoskeletal casualties emerged in the parts of the course with the steepest negative gradients, while a cluster of cardiovascular events was found to occur at the top of the “heartbreak hill,” the longest climb of the race. Regression analysis highlighted the linear relationship between the number of heat and physical exhaustion casualties and the apparent temperature (AT) at 12:00pm (R2 = 0.59; P = .044). This linear equation was used to formulate a model to predict these casualties.

Conclusion:

The findings of this study demonstrate the relationship between meteorological conditions, geographic conditions, and casualties. This will assist planners of other similar events to determine optimum allocation of resources to anticipated injury and illness burden.
In-transit cold treatment: a case of institutional path dependence
Authors: Lubello Pasquale , Codron Jean-Marie
Published: February 2020

In-transit cold treatment consists of exposing food commodities – generally fresh agricultural products – to temperatures approaching 0 °C for a variable number of days during shipping in purpose-equipped containers in order to manage the risks of quarantine contamination. In this paper, we show that in-transit cold treatment is frequently required in the international trade of apples potentially affected by Mediterranean fruit fly (Ceratitis Capitata), despite the existence of potentially less costly and equally effective alternative means of applying the same treatment, in particular ‘cold storage’. We then try to understand why these alternative methods do not emerge spontaneously or become more widespread. We suggest that technical aspects and their respective costs are not always the most important factors. Transaction costs may also come into play. In accordance with ‘institutional path dependence’ literature, we suggest that the negotiation costs a country has to bear in order to encourage its trading partners to adopt an alternative treatment are high enough for it to be preferable to continue using the current solution, despite its higher adoption cost.
Confirmation and characterization of cyhalofop-butyl–resistant Chinese sprangletop (Leptochloa chinensis) populations from China
Authors: Peng Yajun , Pan Lang , Liu Ducai , Cheng Xiaomei , Ma Guolan , Li Sifu , Liu Xueyuan , Wang Lifeng , Bai Lianyang
Published: February 2020

Chinese sprangletop [Leptochloa chinensis (L.) Nees] is one grass weed severely affecting rice (Oryza sativa L.) growth in paddies in China. Cyhalofop-butyl is the main herbicide used to control grass weeds in Chinese paddy fields, especially for controlling L. chinensis; however, L. chinensis has evolved resistance to cyhalofop-butyl due to continuous and extensive application. To investigate cyhalofop-butyl resistance levels and mechanisms in L. chinensis in some of the Chinese rice areas, 66 field populations were collected and treated with cyhalofop-butyl. Of these tested populations, 10 showed a high level of resistance to cyhalofop-butyl; the 50% effective dose ranged within 108.4 to 1,443.5 g ai ha−1 with resistance index values of 9.1 to 121.8 when compared with the susceptible population. Acetyl-coenzyme A carboxylase genes (ACCase) of susceptible and all 10 resistant populations were amplified and sequenced. Among them, Ile-1781-Leu, Trp-2027-Cys, Trp-2027-Ser, and Ile-2041-Asn mutations were found in five resistant populations. No known resistance-related mutations were found in the other five resistant populations, indicating that resistance to cyhalofop-butyl in these populations was likely to be endowed by non–target site resistance mechanisms. Notably, the Ile-1781-Leu and Trp-2027-Cys substitutions have previously been reported, but this is the first report of Trp-2027-Ser and Ile-2041-Asn mutations in L. chinensis. Furthermore, three derived cleaved amplified polymorphic sequence methods were developed to rapidly detect these mutations in L. chinensis.
Pore‐Scale Investigation Of Methane Hydrate‐Bearing Sediments Under Triaxial Condition
Authors: Lei Liang , Seol Yongkoo
Published: February 2020

Mechanical behavior of hydrate‐bearing sediments is critical for well stability, reservoir deformation, and sea floor settlement during gas production. Current understanding on the mechanism of hydrate strengthening hosting sediments relies on conceptual models based on idealistic pore‐scale assumptions. Yet pore‐scale study is rare and limited to hydrate‐bearing sediments formed with surrogate guest molecules rather than methane. We present for the first time pore‐scale triaxial test results of methane hydrate‐bearing sediments. Besides the traditional stress‐strain relationship, we further explored (1) sand particle crushing and (2) pressure‐temperature dependent strength variations and (3) creep of hydrate‐bearing sediments. Results show that as hydrate enables the sand skeleton to bear additional loads, the potential of sand crushing upon hydrate dissociation also increases. Strength of hydrate‐bearing sediments decreases as pressure‐temperature condition approaches hydrate phase boundary. Hydrate‐bearing sediments creep and heal with time. The new observations suggest additional complications to be considered during gas production.
Retrievals of Convective Detrainment Heights Using Ground‐Based Radar Observations
Authors: Starzec M. , Mullendore G. L. , Homeyer C. R.
Published: February 2020

To better constrain model simulations, more observations of convective detrainment heights are needed. For the first time, ground‐based S band radar observations are utilized to create a comprehensive view of irreversible convective transport over a 7‐year period for the months of May and July across the United States. The radar observations are coupled with a volumetric radar echo classification scheme and a methodology that uses the convective anvil as proxy for convective detrainment to determine the level of maximum detrainment (LMD) for deep moist convection. The LMD height retrievals are subset by month (i.e., May and July), by morphology (i.e., mesoscale convective system, MCS, and quasi‐isolated strong convection, QISC), and region (i.e., northcentral, southcentral, northeast, and southeast). Overall, 135,890 deep convective storms were successfully sampled and had a mean LMD height of 8.6 km or tropopause‐relative mean LMD height of
4.3 km; however, LMD heights were found to extend up to 2 km above the tropopause. May storms had higher mean tropopause‐relative LMD heights, but July storms contained the highest overall LMD heights that more commonly extended above the tropopause. QISC had higher mean tropopause‐relative LMD heights and more commonly had LMD heights above the tropopause while only a few MCSs had LMD heights above the tropopause. The regional analysis showed that northern regions have higher mean LMD heights due to large amounts of diurnally driven convection being sampled in the southern regions. By using the anvil top, the highest possible convective detrainment heights extended up to 6 km above the tropopause.
GEOS‐S2S Version 2: The GMAO High‐Resolution Coupled Model and Assimilation System for Seasonal Prediction
Authors: Molod Andrea , Hackert Eric , Vikhliaev Yury , Zhao Bin , Barahona Donifan , Vernieres Guillaume , Borovikov Anna , Kovach Robin M. , Marshak Jelena , Schubert Siegfried , Li Zhao , Lim Young‐Kwon , Andrews Lauren C. , Cullather Richard , Koster Randal , Achuthavarier Deepthi , Carton James , Coy Lawrence , Friere Julliana L. M. , Longo Karla M. , Nakada Kazumi , Pawson Steven
Published: February 2020

The Global Modeling and Assimilation Office (GMAO) has recently released a new version of the Goddard Earth Observing System (GEOS) Subseasonal to Seasonal prediction (S2S) system, GEOS‐S2S‐2, that represents a substantial improvement in performance and infrastructure over the previous system. The system is described here in detail, and results are presented from forecasts, climate equillibrium simulations, and data assimilation experiments. The climate or equillibrium state of the atmosphere and ocean showed a substantial reduction in bias relative to GEOS‐S2S‐1. The GEOS‐S2S‐2 coupled reanalysis also showed substantial improvements, attributed to the assimilation of along‐track absolute dynamic topography. The forecast skill on subseasonal scales showed a much improved prediction of the Madden‐Julian Oscillation in GEOS‐S2S‐2, and on a seasonal scale the tropical Pacific forecasts show substantial improvement in the east and comparable skill to GEOS‐S2S‐1 in the central Pacific. GEOS‐S2S‐2 anomaly correlations of both land surface temperature and precipitation were comparable to GEOS‐S2S‐1 and showed substantially reduced root‐mean‐square error of surface temperature. The remaining issues described here are being addressed in the development of GEOS‐S2S Version 3, and with that system GMAO will continue its tradition of maintaining a state‐of‐the‐art seasonal prediction system for use in evaluating the impact on seasonal and decadal forecasts of assimilating newly available satellite observations, as well as evaluating additional sources of predictability in the Earth system through the expanded coupling of the Earth system model and assimilation components.
ROS in cancer therapy: the bright side of the moon
Authors: Perillo Bruno , Di Donato Marzia , Pezone Antonio , Di Zazzo Erika , Giovannelli Pia , Galasso Giovanni , Castoria Gabriella , Migliaccio Antimo
Published: February 2020

Reactive oxygen species (ROS) constitute a group of highly reactive molecules that have evolved as regulators of important signaling pathways. It is now well accepted that moderate levels of ROS are required for several cellular functions, including gene expression. The production of ROS is elevated in tumor cells as a consequence of increased metabolic rate, gene mutation and relative hypoxia, and excess ROS are quenched by increased antioxidant enzymatic and nonenzymatic pathways in the same cells. Moderate increases of ROS contribute to several pathologic conditions, among which are tumor promotion and progression, as they are involved in different signaling pathways and induce DNA mutation. However, ROS are also able to trigger programmed cell death (PCD). Our review will emphasize the molecular mechanisms useful for the development of therapeutic strategies that are based on modulating ROS levels to treat cancer. Specifically, we will report on the growing data that highlight the role of ROS generated by different metabolic pathways as Trojan horses to eliminate cancer cells.
Transcriptome of iPSC-derived neuronal cells reveals a module of co-expressed genes consistently associated with autism spectrum disorder
Authors: Griesi-Oliveira K. , Fogo M. S. , Pinto B. G. G. , Alves A. Y. , Suzuki A. M. , Morales A. G. , Ezquina S. , Sosa O. J. , Sutton G. J. , Sunaga-Franze D. Y. , Bueno A. P. , Seabra G. , Sardinha L. , Costa S. S. , Rosenberg C. , Zachi E. C. , Sertie A. L. , Martins-de-Souza D. , Reis E. M. , Voineagu I. , Passos-Bueno M. R.
Published: February 2020

Evaluation of expression profile in autism spectrum disorder (ASD) patients is an important approach to understand possible similar functional consequences that may underlie disease pathophysiology regardless of its genetic heterogeneity. Induced pluripotent stem cell (iPSC)-derived neuronal models have been useful to explore this question, but larger cohorts and different ASD endophenotypes still need to be investigated. Moreover, whether changes seen in this in vitro model reflect previous findings in ASD postmortem brains and how consistent they are across the studies remain underexplored questions. We examined the transcriptome of iPSC-derived neuronal cells from a normocephalic ASD cohort composed mostly of high-functioning individuals and from non-ASD individuals. ASD patients presented expression dysregulation of a module of co-expressed genes involved in protein synthesis in neuronal progenitor cells (NPC), and a module of genes related to synapse/neurotransmission and a module related to translation in neurons. Proteomic analysis in NPC revealed potential molecular links between the modules dysregulated in NPC and in neurons. Remarkably, the comparison of our results to a series of transcriptome studies revealed that the module related to synapse has been consistently found as upregulated in iPSC-derived neurons—which has an expression profile more closely related to fetal brain—while downregulated in postmortem brain tissue, indicating a reliable association of this network to the disease and suggesting that its dysregulation might occur in different directions across development in ASD individuals. Therefore, the expression pattern of this network might be used as biomarker for ASD and should be experimentally explored as a therapeutic target.
Double-stranded DNA virioplankton dynamics and reproductive strategies in the oligotrophic open ocean water column
Authors: Luo Elaine , Eppley John M , Romano Anna E , Mende Daniel R , DeLong Edward F
Published: February 2020

Microbial communities are critical to ecosystem dynamics and biogeochemical cycling in the open oceans. Viruses are essential elements of these communities, influencing the productivity, diversity, and evolution of cellular hosts. To further explore the natural history and ecology of open-ocean viruses, we surveyed the spatiotemporal dynamics of double-stranded DNA (dsDNA) viruses in both virioplankton and bacterioplankton size fractions in the North Pacific Subtropical Gyre, one of the largest biomes on the planet. Assembly and clustering of viral genomes revealed a peak in virioplankton diversity at the base of the euphotic zone, where virus populations and host species richness both reached their maxima. Simultaneous characterization of both extracellular and intracellular viruses suggested depth-specific reproductive strategies. In particular, analyses indicated elevated lytic interactions in the mixed layer, more temporally variable temperate phage interactions at the base of the euphotic zone, and increased lysogeny in the mesopelagic ocean. Furthermore, the depth variability of auxiliary metabolic genes suggested habitat-specific strategies for viral influence on light-energy, nitrogen, and phosphorus acquisition during host infection. Most virus populations were temporally persistent over several years in this environment at the 95% nucleic acid identity level. In total, our analyses revealed variable distributional patterns and diverse reproductive and metabolic strategies of virus populations in the open-ocean water column.
Conversion of p–n conduction type by spinodal decomposition in Zn-Sb-Bi phase-change alloys
Authors: Wang Guoxiang , Shi Haizhou , Lotnyk Andriy , Shi Daotian , Wang Rongping
Published: February 2020

Phase-change films with multiple resistance levels are promising for increasing the storage density in phase-change memory technology. Diffusion-dominated Zn2Sb3 films undergo transitions across three states, from high through intermediate to low resistance, upon annealing. The properties of the Zn2Sb3 material can be further optimized by doping with Bi. Based on scanning transmission electron microscopy combined with electrical transport measurements, at a particular Bi concentration, the conduction of Zn-Sb-Bi compounds changes from p- to n-type, originating from spinodal decomposition. Simultaneously, the change in the temperature coefficient of resistivity shows a metal-to-insulator transition. Further analysis of microstructure characteristics reveals that the distribution of the Bi-Sb phase may be the origin of the driving force for the p–n conduction and metal-to-insulator transitions and therefore may provide us with another way to improve multilevel data storage. Moreover, the Bi doping promotes the thermoelectric properties of the studied alloys, leading to higher values of the power factor compared to known reported structures. The present study sheds valuable light on the spinodal decomposition process caused by Bi doping, which can also occur in a wide variety of chalcogenide-based phase-change materials. In addition, the study provides a new strategy for realizing novel p–n heterostructures for multilevel data storage and thermoelectric applications.
Post-functionalization of dibenzothiophene to functionalized biphenyls via a photoinduced thia-Baeyer-Villiger oxidation
Authors: Ma Xiaofeng , Liu Yazhou , Du Le , Zhou Jingwei , Markó István E.
Published: February 2020

The Baeyer-Villiger reaction is used extensively in organic chemistry. Sila- and bora-variants have also been documented widely, with these processes underpinning, for example, the Fleming-Tamao oxidation and hydroborative alkene hydration, respectively. By contrast, the development of thia-Baeyer-Villiger reactions involving sulfoxides has long been considered unlikely because competitive oxidation to the sulfone occurs exclusively. Here, we disclose a photoinduced thia-Baeyer-Villiger-type oxidations; specifically, we find that exposure of dibenzothiophene (DBT) derivatives to an iron porphyrin catalyst under Ultraviolet irradiation in the presence oft-BuOOH generates sulfinic esters in up to 87% yield. The produced sulfinic esters are transformed to a variety of biphenyl substrates including biphenyl sulfoxides, sulfones and sulfonamides in 1-2 steps. These results provide a mild process for the selective functionalization of sulfur compounds, and offer a biomimetic approach to convert DBT into 2-hydroxybiphenyl under controllable stepwise pathway. Based upon experimental evidences and DFT calculation, a mechanism is proposed.
Evolutionary games with environmental feedbacks
Authors: Tilman Andrew R. , Plotkin Joshua B. , Akçay Erol
Published: February 2020

Strategic interactions arise in all domains of life. This form of competition often plays out in dynamically changing environments. The strategies employed in a population may alter the state of the environment, which may in turn feedback to change the incentive structure of strategic interactions. Feedbacks between strategies and the environment are common in social-ecological systems, evolutionary-ecological systems, and even psychological-economic systems. Here we develop a framework of ‘eco-evolutionary game theory’ that enables the study of strategic and environmental dynamics with feedbacks. We consider environments governed either by intrinsic growth, decay, or tipping points. We show how the joint dynamics of strategies and the environment depend on the incentives for individuals to lead or follow behavioral changes, and on the relative speed of environmental versus strategic change. Our analysis unites dynamical phenomena that occur in settings as diverse as human decision-making, plant nutrient acquisition, and resource harvesting. We discuss implications in fields ranging from ecology to economics.
Insights into the aetiology of snoring from observational and genetic investigations in the UK Biobank
Authors: Campos Adrián I. , García-Marín Luis M. , Byrne Enda M. , Martin Nicholas G. , Cuéllar-Partida Gabriel , Rentería Miguel E.
Published: February 2020

Although snoring is common in the general population, its aetiology has been largely understudied. Here we report a genetic study on snoring (n ~ 408,000; snorers ~ 152,000) using data from the UK Biobank. We identify 42 genome-wide significant loci, with an SNP-based heritability estimate of ~10% on the liability scale. Genetic correlations with body mass index, alcohol intake, smoking, schizophrenia, anorexia nervosa and neuroticism are observed. Gene-based associations identify 173 genes, including DLEU7, MSRB3 and POC5, highlighting genes expressed in the brain, cerebellum, lungs, blood and oesophagus. We use polygenic scores (PGS) to predict recent snoring and probable obstructive sleep apnoea (OSA) in an independent Australian sample (n ~ 8000). Mendelian randomization analyses suggest a potential causal relationship between high BMI and snoring. Altogether, our results uncover insights into the aetiology of snoring as a complex sleep-related trait and its role in health and disease beyond it being a cardinal symptom of OSA.
Immunoproteasome expression is associated with better prognosis and response to checkpoint therapies in melanoma
Authors: Kalaora Shelly , Lee Joo Sang , Barnea Eilon , Levy Ronen , Greenberg Polina , Alon Michal , Yagel Gal , Bar Eli Gitit , Oren Roni , Peri Aviyah , Patkar Sushant , Bitton Lital , Rosenberg Steven A. , Lotem Michal , Levin Yishai , Admon Arie , Ruppin Eytan , Samuels Yardena
Published: February 2020

Predicting the outcome of immunotherapy treatment in melanoma patients is challenging. Alterations in genes involved in antigen presentation and the interferon gamma (IFNγ) pathway play an important role in the immune response to tumors. We describe here that the overexpression of PSMB8 and PSMB9, two major components of the immunoproteasome, is predictive of better survival and improved response to immune-checkpoint inhibitors of melanoma patients. We study the mechanism underlying this connection by analyzing the antigenic peptide repertoire of cells that overexpress these subunits using HLA peptidomics. We find a higher response of patient-matched tumor infiltrating lymphocytes against antigens diferentially presented after immunoproteasome overexpression. Importantly, we find that PSMB8 and PSMB9 expression levels are much stronger predictors of melanoma patientsʼ immune response to checkpoint inhibitors than the tumors’ mutational burden. These results suggest that PSMB8 and PSMB9 expression levels can serve as important biomarkers for stratifying melanoma patients for immune-checkpoint treatment.
CASZ1 induces skeletal muscle and rhabdomyosarcoma differentiation through a feed-forward loop with MYOD and MYOG
Authors: Liu Zhihui , Zhang Xiyuan , Lei Haiyan , Lam Norris , Carter Sakereh , Yockey Oliver , Xu Max , Mendoza Arnulfo , Hernandez Edjay R. , Wei Jun S. , Khan Javed , Yohe Marielle E. , Shern Jack F. , Thiele Carol J.
Published: February 2020

Embryonal rhabdomyosarcoma (ERMS) is a childhood cancer that expresses myogenic master regulatory factor MYOD but fails to differentiate. Here, we show that the zinc finger transcription factor CASZ1 up-regulates MYOD signature genes and induces skeletal muscle differentiation in normal myoblasts and ERMS. The oncogenic activation of the RAS-MEK pathway suppresses CASZ1 expression in ERMS. ChIP-seq, ATAC-seq and RNA-seq experiments reveal that CASZ1 directly up-regulates skeletal muscle genes and represses non-muscle genes through affecting regional epigenetic modifications, chromatin accessibility and super-enhancer establishment. Next generation sequencing of primary RMS tumors identified a single nucleotide variant in the CASZ1 coding region that potentially contributes to ERMS tumorigenesis. Taken together, loss of CASZ1 activity, due to RAS-MEK signaling or genetic alteration, impairs ERMS differentiation, contributing to RMS tumorigenesis.
Room-temperature terahertz anomalous Hall effect in Weyl antiferromagnet Mn3Sn thin films
Authors: Matsuda Takuya , Kanda Natsuki , Higo Tomoya , Armitage N. P. , Nakatsuji Satoru , Matsunaga Ryusuke
Published: February 2020

Antiferromagnetic spin motion at terahertz (THz) frequencies attracts growing interests for fast spintronics, however, their smaller responses to external field inhibit device application. Recently the noncollinear antiferromagnet Mn3Sn, a Weyl semimetal candidate, was reported to show large anomalous Hall effect (AHE) at room temperature comparable to ferromagnets. Dynamical aspect of such large responses is an important issue to be clarified for future THz data processing. Here the THz anomalous Hall conductivity in Mn3Sn thin films is investigated by polarization-resolved spectroscopy. Large anomalous Hall conductivity $${\mathrm{Re}}\;\sigma _{xy}\left( \omega \right) \sim 20\;{\mathrm{\Omega }}^{ - 1}{\mathrm{cm}}^{ - 1}$$
Re

σ

x
y

ω

~
20

Ω

−
1

cm

−
1

at THz frequencies is clearly observed as polarization rotation. A peculiar temperature dependence corresponding to the breaking/recovery of symmetry in the spin texture is also discussed. Observation of the THz AHE at room temperature demonstrates the ultrafast readout for the antiferromagnetic spintronics using Mn3Sn, and will also open new avenue for studying nonequilibrium dynamics in Weyl antiferromagnets.
In vitro self-replication and multicistronic expression of large synthetic genomes
Authors: Libicher K. , Hornberger R. , Heymann M. , Mutschler H.
Published: February 2020

The generation of a chemical system capable of replication and evolution is a key objective of synthetic biology. This could be achieved by in vitro reconstitution of a minimal self-sustaining central dogma consisting of DNA replication, transcription and translation. Here, we present an in vitro translation system, which enables self-encoded replication and expression of large DNA genomes under well-defined, cell-free conditions. In particular, we demonstrate self-replication of a multipartite genome of more than 116 kb encompassing the full set of Escherichia coli translation factors, all three ribosomal RNAs, an energy regeneration system, as well as RNA and DNA polymerases. Parallel to DNA replication, our system enables synthesis of at least 30 encoded translation factors, half of which are expressed in amounts equal to or greater than their respective input levels. Our optimized cell-free expression platform could provide a chassis for the generation of a partially self-replicating in vitro translation system.