bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Various Thawing Temperatures of Frozen Semen on the Success of Artificial Insemination of Beef Cattle
Authors: Wulandari Ida Arlita , Prihatno Surya Agus
Published: November 2022

The study about the effect of various thawing temperatures frozen semen on the success of artificial insemination of beef cattle had been conducted in Residency of Sleman Territory of Yogyakarta. The study used 59 heifers and cows that were oestrus (without oestrus stimulation) with body condition score (BCS) of 3 and 1,5-8 years age. They were inseminated using frozen semen of Simmental thawed in temperature of 37°C, 35°C (warm water) and 28-30°C (cold water). Effect of thawing temperatures was evaluated by non returnrate (NR). NR of thawing temperatures of 37°C, 35°C and 28-30°C were 63,16%, 55%, and 45%. Thawing temperature of 37°C increased the non returnrate compared to the thawing temperatures of 35°C and 28-30°C. Statistical analysis by Chi-square showed there were no differences between thawing temperatures of  37°C, 35°C and 28-30°C on the success of artificial insemination
Inhibitory Effect of Extract Granule of Earthworms (Lumbricus rubellus) on the Pathogenic Bacteria In Vitro
Authors: Istiqomah Lusty , Damayanti Ema , Julendra Hardi , Istika Dewi , Winarsih Sri
Published: November 2022

The objective of this study was to determine the inhibition ability of the earthworm (L. rubellus) extract (ECT), dried earthworm extract (ECT-k), and granule earthworm extract (ECT-g) as poultry feed additive against some pathogenic bacteria. Antibacterial activity was performed using diffusion method against Escherichia coli, Salmonella pullorum, Pseudomonas aeruginosa, and Staphylococcus aureus  in vitro. In the present study, the concentrations of ECT, ECT-k, and ECT-g in nutrient broth (NB) media tested were consisted of treatments A: 0%, B: 0.26%, C: 0.52%, D: 0.78% and E: 1.04% (g/vol) respectively. The results of the in vitro study showed that started from ECT level 0.26% inhibited (P <0.05) growth of P. aeruginosa and S. aureus, while ECT level 0.52% inhibited (P <0.05) E. coli and S. pullorum which proportional to the increased in concentration. ECT-k level 0.26% inhibited (P <0.05) growth of E. coli and S. aureus, while ECT-k level 0.52% inhibited (P <0.05) P. aeruginosa, and ECT-k level 1.04% inhibited (P<0.05) growth of S. pullorum. ECT-g level 0.26% inhibited (P <0.05) growth of S. pulorum, while ECT-g level 0.52% inhibited (P <0.05) S. aureus and ECT-g level 1.04% inhibited (P<0.05) growth of P. aeruginosa. There were no antibacterial action (P>0.05) of ECT and ECT-t against S. pullorum. Diameter of inhibition zone for 24 hours showed that S. aureus was the most sensitive bacterium to ECT and ECT-k, and S. pullorum was the most sensitive bacterium to ECT-g.
The In Vitro Study: Anti Aeromonas hydrophila of Ethanol Extract of Kamboja Leaves (Plumeria alba)
Authors: . Ikrom , T.R. Denok Asih , A. Reni Wira , B Bintang Perkasa , N. Rafika Tiara , . Wasito
Published: November 2022

Motile aeromonas septicemia (MAS) caused by Aeromonas hydrophila could attack seriously freshwater fish in Indonesia.  Its mortality rate was reported to be 100% on fish population. According to the Commission of Healthy Fish and National Environment, the MAS has been determined as one of major diseases in Indonesia.  However, its therapy using antibiotics is still difficult because the MAS has so many different strains and can be resistant to drugs. If the antibiotics given over the maximal limit this will be able to make the fish (food products) is unsafe for human consumption. One effort to do is by using a traditional herbal medicine. Kamboja leaves is reported to contain flavonoid as antibacterial agent. Therefore, this research is aimed to determine the effectiveness of kamboja leaves extract as antibacterial agent using diffusion and dilution method in vitro. Inhibition zone of diffusion method and bacterial growth are observed by diffusion and dilution approaches, rescpectively. The results of  the present study showed that 8% concentration of kamboja leaves extract was good enough to prevent bacterial growth by dilution method, whereas by diffusion method, it needs 100% of the extract concentration. The zones of growth inhibition is 1.307 cm cm which is almost the same as the diameter (1,637 cm.)  achieved by using  30 µg tetracycline. The zones of bacterial growth inbihition due to  the extract still persists on the day 10. But, for the antibiotic, the the zones of bacterial growth inbihition is already faded  starting from the day 4. Therefore, it is concluded that kamboja leaves extact  is more potent than that of antibiotic (tetracycline)  in inhibiting bacterial growth in vitro.
HISTOLOGY AND ELECTRONE MICROSCOPIC STUDY OF THE EFFECTS OF AN GnRH AGONIST ON THE CANINE TESTIS AND PROSTATE AFTER 3 MONTHS OF TREATMENT
Authors: Junaidi Aris
Published: November 2022

Using light and electrone microscopic techniques the structure of the testicle, epididymis and prostate gland of mature male dogs were studied 3 months after implantation with a slow release implant containing 6 mg GnRH agonist deslorelin. After 3 months of treatment, all tubules seminiferous showed atropic and aspermatogenic. Atrophy of the Leydig cells was observed in the interstitial tissue. The glandular epithelium of the prostate showed atrophy and was non secretory. At the electrone microscopic levels, Sertoli and Leydig cells showed a marked atrophy and their spermatogenic tubules were mostly lined with Sertoli cells at the basal lamina. The prostate gland showed atrophy of the nucleous and the epithelium was non secretory. The present data showed that a slow release implant containing GnRH agonist deslorelin can be used effectively to suppress fertility in male dogs.
IDENTIFICATION OF SERPTICAEMIA EPIZOOTICA (SE) ON SWAMP BUFFALOES (KALANG BUFALLOES) IN SOUTH KALIMANTAN
Authors: Utomo Bambang Nganji , Tarmudji Tarmudji , Hamdan A.
Published: November 2022

Identification of Septicaemia Epizootica (SE) was carried out on swamp buffaloes at the districts of Hulu Sungai Tengah (HST) and Hulu Sungai Utara (HSU) and those which were slaughtered in the abbatoars of Banjarmasin, Banjarbaru and Amuntai, South Kalimantan. This study was conducted to find out whether Septicaemia Epizootica was present among the swamp buffaloes population. Thirty two samples of swab nasopharynx from the field (HSU and HST) and 60 samples from the abbatoars were collected and checked toward Pasteurella multocida. And 45 samples of sera were examined for antibody with Elisa method. Based on bacterio­logical examination there was no Pasteurella multocida could be identified from the samples and from Elisa examination was also negative from Septicaemia Epizootica.
The Susceptibility Of Burkholderia Pseudomallei Strains To Various Chemotherapeutics : a review
Authors: Indriana Jeanne
Published: November 2022

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MONOCLONAL ANTIBODIES AGAINST OUTER MEMBRANE PROTEIN PASTEURELLA MULTOCIDA STRAIN DOMPU
Authors: Murwani Sri , Asmara Widya
Published: November 2022

Monoclonal antibodies were produced against outer membrane protein of Pasteurella multocida strain Dompu. The bacterial isolate was obtained from [he bacteria! collection held at Microbiology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Irs purity and patogenicily has beeen tested.Killed PatTeurella multocida were used ro immunized 7 female, 8-10 week old of Balb/ c mice. Cell fusion between limphocytes and myeloma cells (2:1) were carried out by PEG stimulation and bybrids were cultured in  HAT medium.The OMP were prepared from sonicated organism using centrifugallon in the presense of sarcosyL These antigens were than used to select antibody producing hvbridomas ELISA and cloning of hybridomas were done by serial dilution method.Four hybridomas clones were obtained, till producing monoclonal antibodies which reacted specifically against OMP Pasietirelta multacida stain Dompu.
THE EFFECT OF CADMIUM CHLORIDE TO THE STERILITY OF MALE RAT
Authors: Tato Syarifuddin , Sari Puspa Wikan
Published: November 2022

The effect of cadmium chloride (CdO2) to the sterility of male rat have been observed to the first generation (Fj).Sixty white rats consisted of 30 male rats at 3 weeks old divided Into 6 groups and were given CdCl2 orally ad libitum for 42 days, Tl»e concentration of CdCl2 for each group was 0 ppin (group I), 200 ppm (group H), 100 ppm (group ID), 50 ppm (group IV), 25 ppm (group V) and 12,5 ppm (group VI). After treatments, aii male rats were mated with female rats by mixing a couple of rats in a cage for 7 days. Then male rats were kiiied all to examine the histopathoiogy of their testis. The female rats were kept until 18 days of pregnancy, then all were killed. The percentage of pregnancy and the number of baby in each female rat were counted. The histopamoiogy of seminiferous tubules of testis were analysed qualitatively.The results showed that CdCl2 at 12,5 and 25 ppm caused 20% and 40% of infertility. 50 ppm of CdCfe caused 100% of infertility and necrosis of spermatocyte and spermaride celk; there no spematozoa in the seminiferous tubules.
CROSS-SECTIONAL STUDY ON CAPRINE SARCOPTIC SCABIES IN THE DISTRIC OF Kl LONPROGO
Authors: Samkhan Samkhan , Budiharta Setyawan , Darjoprawito Wardiarto , Sumiarto Bambang
Published: November 2022

A cross-sectional study of scabies was performed on 2615 goats from 492 small holder farmers, covered aC subdistricts and 40 out of 88 vfllages in Kulanprogo district, Yogyakarta Province. The Objectives of this study is to investigate (a) factors associated with the prevalence of the disease in goat, and (b) the prevalence uf thedisease among goat population in the district A combination of a three stage and duster sampling procedures was used to select the goat. An animal was considered as suffering from scabies if it showed cuaical sign of scabies and positive laboratory finding of Sarcoptes scabieLThe mportant findings of this study include significant association between sarcoptic scabies in the farm and cleanliness of the barn, animal density, new mcoming animal, new annual from outside of the village, intensive barn, separation of younger animal, between barn distance and closed barn. Other factos, Le. altitude, former's formal education and experience of goat raising are not significantly associated with the disease. In the animal level, new incoming animal, clinical signs, previous infestation and age are found to be associated with the occurrence of the disease. No significant association is found between the occurrence of sarcoptic scabies and sex and breed. The prevalence of the disease is found o be 93% (467492) and 73% (205/2615) for farm and animal level, i respectively.
THE USE OF DIFFERENT STAINING METHODS TO EVALUATE THE DEVELOPMENT AND VIABILITY OF BOVINE EMBRYOS OBTAINED IN VIVO AND IN VITRO
Authors: Gustari Sri , Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome
Published: November 2022

In an attempt to find a good method to evaluate embryos we classified the in vitro embryos into freezable and non-freezable by subjective method. These embryos were compared with in vivo freezable embryos using Giemsa staining. The embryo development and viability was evaluated by fluorescent stain (PDA and Hoechst 33342) and compared with the result after Giemsa stain.That number of cells in freezable in vitro and in vivo embryos were not different (P > 0.1), while in vitro fresszable and non-freezable embryos showed significantly differences (P < 0,001) in number of cells.The agreement between Hoechst 33342 and Giemsa stain was quite high (63%) in embryos with number of cells less than 20, and low agreement (25 - 34 %) in embryios with number of cells more than 20. There was a significantly correlation (0.82) betwen the degree of fluorescent of FDA stain and the number of cells at Giemsa stain.The subjective classification of in vitro embryos can be used in the practical situation. PDA staining is a good tool to check this classification as well as to evaluate the development and viability of embroys in different step of experimental trials. Hoechst can be a valuable
The Small-scale Peasant Economy in the French German Peasant Problem
Authors: Yin Jie
Published: November 2022

Capitalist socialized mass production has continuously squeezed the production environment of farmers, increased productivity and bankrupted farmers have also increased, and more farmers come to the city but can not stand firm in the city. In this regard, Engels believed that small-scale peasant household production was no longer suitable for the needs of economic development. He wrote the “French German Peasants’ Problem” to discuss his ideas about farmers taking the road of cooperative production, leaving valuable inspiration for the development of farmers, rural areas and agriculture in today’s social development.
Growth and Upheaval in the Network Media Economy in Canada, 1984-2021
Authors: Winseck Dwayne
Published: November 2022

The Canadian contribution and data set prepared as part of the Global Media and Internet Concentration (GMIC) project offers an independent academic, empirical and data-driven analysis of a deceptively simple yet profoundly important question: have telecom, media and internet markets become more concentrated over time, or less? Media Ownership and Concentration is presented from more than a dozen sectors of the telecom-media-internet industries, including film, music and book industries.
Dependence of the microstructure and mechanical properties of cold-resistant steel sheets with a guaranteed yield strength of 420 MPa on the thermomechanical processing
Authors: Knyazyuk T. V. , Motovilina G. D. , Novoskoltsev N. S. , Kondratiev N. A.
Published: November 2022

For the production of sheets with a thickness of 5 and 15 mm from cold-resistant steel with a guaranteed yield strength of 420 MPa, continuous rolling was developed and tested in laboratory conditions. The present study considers the influence of the parameters of thermal deformation treatment (temperature of the end of rolling and cooling rate) on the formation of the final microstructure. The mechanical properties of the sheets were analyzed taking into account the formed structural features. Technological recommendations for hot rolling of low-carbon low-alloy steel sheets with a guaranteed yield strength of 420 MPa are proposed.
Welding of ultra-high molecular weight polyethylene and its composite to increase the sealing of lining coatings
Authors: Sokolova M. D. , Chirikov A. A. , Gogoleva O. V. , Zyryanov I. V.
Published: November 2022

This article proposes the use of magnetic induction for ultra-high molecular weight polyethylene (UHMWPE) welding. The conducted mechanical and structural studies confirm the high efficiency of the method of welding ultra-high molecular weight polyethylene and its composite using magnetic induction with a ferromagnet in the form of a steel mesh. The chosen optimal welding mode allows obtaining a high-quality weld. It has been established that the preservation of the strength properties of the weld at the selected welding mode at the bulk level of UHMWPE is due to the controlled local heating of the material in the welding zone and increased adhesion at the UHMWPE-metal mesh interface.
Study of the structure and properties of the metal of reactor pressure vessel welded joints made of Cr–Mo–V steel in the process of manufacture and operation
Authors: Timofeev M. N. , Galyatkin S. N.
Published: November 2022

The paper presents a study of changes in the structure and mechanical properties of the metal of the welded joints of steel 15Cr2МoVА-А mod. A during post weld heat treatment (PWHT) at the reactor pressure vessel (RPV) manufacturing and also as a result of thermal exposure during its operation. It has been established that in as-welded state, the weld metal has a satisfactory level of plastic characteristics and high values of impact strength, but an excessively high level of strength properties. PWHT at 655°C reduces the strength properties, but does not lead to a significant increase of impact strength level. The final properties are formed by PWHT at 670°C. At the indicated temperatures, the low sensitivity of the heat affected zone (HAZ) metal and the base metal to tempering as well as to thermal exposure at 350°C, has been revealed.
Influence of macrostructure and phase composition on performance characteristics of welded joints of WWER type reactor vessels
Authors: Maltsev D. A. , Kuleshova E. A. , Fedotov S. V. , Vasilenko V. V. , Bubyakin S. A.
Published: November 2022

The influence of the grain structure and phase composition of welded seams of WWER-type reactors on their operational characteristics in the initial state is analyzed. Comprehensive studies of welds with various structures formed as a result of welding using various technologies have been carried out. It is shown that differences in the phase composition and morphology of the grain structure of the weld metal in the initial state, which is characterized by an increased proportion of columnar grains and a higher density of carbide phases, lead to an increase in the yield strength and critical brittleness temperature of welded joints.
Synthesis of nanoporous functional materials for the chemical industry
Authors: Memetova A. E. , Zelenin A. D. , Memetov N. R. , Pasko T. V. , Gerasimova A. V. , Tarov D. V.
Published: November 2022

In this study, we synthesized samples of nanoporous carbon materials (NCM) from polymer raw materials. The influence of the conditions of the synthesis process (the mass ratio of the activating agent to the precursor) on the structure of the obtained samples has been studied. Varying the mass ratio of the activating agent to the precursor made it possible to obtain microporous, micromesoporous, and mesoporous carbon materials. Methane adsorption has been researched in a wide pressure range. The highest adsorption of methane, equal to ≈ 20 mmol/g at 100 bar and 298 K, is achieved on a sample with a ratio of the activating agent KOH to carbonized precursor 6:1 (6NCM).
The influence of natural shungite on the properties and structure of polytetrafluoroethylene
Authors: Ammosova A. P. , Ushkanov A. A. , Sleptsova S. A. , Okhlopkova A. A. , Lazareva N. N.
Published: November 2022

The paper presents the results of physical-mechanical and tribological studies of composites based on polytetrafluoroethylene and natural shungite. It has been established that the introduction of shungite leads to an increase in the wear resistance of the material by 114 times compared to an unfilled polymer. Electron microscopy has shown that a secondary layer is formed on the friction surface of the composites, which protects the material from wear. Using IR spectroscopy, it has been established that during the wear of composites, tribochemical reactions occur with the formation of oxygen-containing functional groups and subsequent structuring of the surface layer. The results of the study obtained by differential scanning calorimetry show that the presence of natural shungite in the PTFE matrix leads to ordering of the structure of the composites.
Anodic behavior of gallium doped Zn55Al alloy in acid, neutral and alkaline environments
Authors: Sirojidinov M. E. , Ganiev I. N. , Sharipov J. H. , Obidov Z. R.
Published: November 2022

The paper presents results of potentiostatical and potentiodynamical research of anode behaviour of gallium-doped Zn55Al alloy in acid, neutral and alkaline environments of electrolytes HCl, NaCl and NaOH, at various pH values. Gallium additives (0.01-1.0 wt%) lead to displacement of corrosion electrochemical potential, pitting formation and repassivity to positive values. The results indicate a decrease in the corrosion rate of gallium doped alloys by 2-3 times compared to the base alloy. Such dependence is observed in all investigated corrosion environments.
Hydraulic vulnerability segmentation between branches and roots increases with environmental aridity
Authors: Ye Qing , Tang Weize , Liu Hui , Liang Xingyun , He Pengcheng , Zhao Han , Liu Xiaorong
Published: November 2022

The hydraulic vulnerability segmentation hypothesis proposes that plant
branches are more resistant to cavitation than roots, namely, the
difference in vulnerability to cavitation between branches and roots is
positive ( P > 0).
However, it is not clear how this phenomenon can vary along
environmental aridity gradients. We compiled the above hypothesis with
105 woody species from four biomes with increasing aridity, by compiling
functional traits related to hydraulic properties and anatomical
structures of branches and roots. We investigated the relationships
between P and several
environmental factors that are associated with aridity. We found a
positive P across species, which
supported the hydraulic vulnerability segmentation hypothesis, and
P increased significantly with
environmental aridity. Branch xylem hydraulic conductivity changed from
“more efficient” (e.g., wider conduit, higher hydraulic conductivity)
to “safer” (e.g., narrower conduit, more negative P) in response to increased aridity, while root xylem
hydraulic conductivity remained unchanged across aridity gradients. Our
results demonstrated that hydraulic vulnerability segmentation is more
pronounced for species from arid regions. Changes in branch traits may
be responsible for hydraulic vulnerability segmentation between branches
and roots.
Complete mitochondrial genome of Sineleotris saccharae (Perciformes, Odontobutiae) and phylogenetic analysis
Authors: Zhou Liying , Wang Minghua , Li Daming , Tang Shengkai , Liu Yanshan , Chen Xiaohui , Zhong Liqiang
Published: November 2022

The freshwater sleeper, Sineleotris saccharae, endemic to southeastern
of China belongs to the family of Odontobutiae. The composition of the
Odontobutidae is controversial due to the lack of molecular data.
Information about genetic characteristics of S. saccharae was still not
reported. In the present study, we determined the complete mitochondrial
genome of S. saccharae for the first time and analyzed its evolutionary
relationship. The complete mitochondrial genome of S. saccharae was
16,487 bp in length, and consisted of 13 protein-coding genes (PCGs), 22
transfer RNAs (tRNAs), 2 ribosomal RNA (rRNAs) and a control region
(CR). The gene arrangement and orientation of S. saccharae mitogenome
was identical to those of other teleosts. Phylogenetic analysis by
Maximum likelihood (ML) methods strongly supported the monophyly of
Odontobutidae containing two clades. The genus Odontobutis was sister to
the clade consisting of Perccottus and Neodontobutis. Meanwhile,
Microdous, Sineleotris and Micropercops formed another clade. These data
will be helpful for understanding the genetic information of S.
saccharae and systematics of the odontobutids.
Results from a test-and-treat study for influenza among residents of homeless shelters in King County, WA: a stepped-wedge cluster-randomized trial.
Authors: Rogers Julia , Casto Amanda , Nwanne Gift , Link Amy , Martinez Miguel , Nackviseth Callista , Wolf Caitlin , Hughes James , Englund Janet , Sugg Nancy , Uyeki Timothy M , Han Peter , Pfau Brian , Shendure Jay , Chu Helen
Published: November 2022

Background Persons experiencing homelessness face increased risk of
influenza as overcrowding in congregate shelters can facilitate
influenza virus spread. Data regarding on-site influenza testing and
antiviral treatment within homeless shelters remains limited. Methods We
conducted a cluster-randomized stepped-wedge trial of point-of-care
molecular influenza testing coupled with antiviral treatment with
baloxavir or oseltamivir in residents of 14 homeless shelters in
Seattle, WA, USA. Residents ≥3 months with cough or ≥2 acute respiratory
illness (ARI) symptoms and onset <7 days were eligible. In
control periods, mid-nasal swabs were tested for influenza by RT-PCR.
The intervention period included on-site rapid molecular influenza
testing and antiviral treatment for influenza-positives if symptom onset
was <48 hours. The primary endpoint was monthly influenza
virus infections in the control versus intervention period. Influenza
whole genome sequencing was performed to assess transmission and
antiviral resistance. Results During 11/15/2019–4/30/2020, and
11/2/2020–4/30/2021, 1,283 ARI encounters from 668 participants were
observed. Influenza virus was detected in 51 (4%) specimens using
RT-PCR, (A=14; B=37); 21 influenza virus infections were detected from
269 (8%) of intervention-eligible encounters by rapid molecular testing
and received antiviral treatment. 37% of ARI-participant encounters
reported symptom onset <48 hours. The intervention had no
effect on influenza virus transmission (adjusted relative risk 1.73,
95% CI 0.50–6.00). Of 23 influenza genomes, 86% of A(H1N1)pdm09 and
81% of B/Victoria sequences were closely related. Conclusion Our
findings suggest feasibility of influenza test-and-treat strategies in
shelters. Additional studies would help discern an intervention effect
during periods of increased influenza activity
Sensitivity to long days for flowering is reduced in Arabidopsis by yearly variation in growing season temperatures
Authors: Fjellheim Siri , Kinmonth-Schultz Hannah , Sønstebø Jørn H. , Croneberger Andrew J. , Johnsen Sylvia S. , Leder Erica , Lewandowska-Sabat Anna , Imaizumi Takato , Rognli Odd Arne , Vinje Hilde , Ward Joy K.
Published: November 2022

Conservative flowering behaviors, such as flowering during long days in
summer or late flowering at a high leaf number, are often proposed to
protect against variable winter and spring temperatures which lead to
frost damage if premature flowering occurs. Yet, due the many factors in
natural environments relative to the number of individuals compared,
assessing which climate characteristics drive these flowering traits has
been difficult. We applied a multidisciplinary approach to ten
winter-annual Arabidopsis thaliana populations originating along
a wide climactic gradient in Norway. We used a variable reduction
strategy to assess which of 100 climate descriptors from their home
sites correlated most to their behaviors when grown in common garden and
assessed sequence variation of 19 known environmental-response flowering
genes. Photoperiod sensitivity inversely correlated with interannual
variation in timing of growing season onset (start of favorable spring
temperatures). Time to flowering appeared driven by growing season
length, curtailed by cold fall temperatures. The distribution of
FLM, TFL2, and HOS1 haplotypes, genes involved in
ambient temperature response, correlated with growing-season climate. We
show that long-day sensitivity and late flowering may be driven not by
risk of spring frosts, but by growing season temperature and length
perhaps to opportunistically maximize growth.
Contemporary issues, current best practice and ways forward in soil protist ecology
Authors: Geisen Stefan , Lara Enrique , Mitchell Edward
Published: November 2022

Soil protists are increasingly studied due to a release from previous
methodological constraints and the acknowledgement of their immense
diversity and functional importance in ecosystems. However, these
studies often lack a sufficient depth in knowledge, which is visible in
the form of falsely used terms and false- or over-interpreted data with
conclusions that cannot be drawn from the data obtained. As we welcome
that also non-experts include protists in their still mostly bacterial
and/or fungal focused studies, our aim here is to help avoid some common
errors. We provide an overview of current terms to be used when working
on soil protists, like protist instead of protozoa, predator instead of
grazer, microorganisms rather than microflora and terms to be used to
describe the prey spectrum of protists. We then highlight some do’s and
don’ts in soil protist ecology including challenges related to
interpreting 18S rRNA gene amplicon sequencing data. We caution against
the use of standard bioinformatic settings optimized for bacteria and
the uncritical reliance on incomplete and partly erroneous reference
databases. We also show why causal inferences cannot be drawn from
sequence-based correlation analyses or any sampling/monitoring, study in
the field without thorough experimental confirmation and sound
understanding of the biology of taxa. Together, we envision this work to
help non-experts to more easily include protists in their soil ecology
analyses, and obtain more reliable interpretations from their protist
data and other biodiversity data that, in the end, will help to better
understand soil ecology.
INFLUENCE OF SCAT AGEING ON THE GUT MICROBIOME
Authors: Oliveros Alejandro , Terraube Julien , Levengood Alexis , Powell Daniel , Frere Celine
Published: November 2022

The study of the host-microbiome by the collection of non-invasive
samples has the potential to become a powerful tool for conservation
monitoring and surveillance of wildlife. However, multiple factors can
bias the quality of data recovered from scats, particularly when
field-collected samples are used given that the time of defecation is
generally unknown and could have been as recent as hours, days, or
weeks. Previous studies using scats have shown that exposure to aerobic
conditions can compromise the microbial composition and that this rate
of exposure differs between species. However, the impact that this aging
process has on the relationship between the bacterial and fungal
composition has yet to be explored. In this study, we measured the
effects of time post-defecation on bacterial and fungal compositions and
structures in a controlled experiment using scat sample from the
endangered koala (Phascolarctos cinereus). We found that targeting the
core of the scat for DNA extraction reduced the impact of oxygen
exposure as we did not observe the previously reported reduction in
obligate anaerobic bacteria nor an increase in facultative anaerobes
even after aging for 10 days. We found that even though bacteria remain
stable through the scat aging process, the fungal composition did not.
We report a cluster of fungal taxa that colonises scats after defecation
which can dilute the genetic material from the autochthonous mycoflora
and inhibit recovery. Finally, we propose strategies to combat the
effects of time and preserve the integrity of a scat sample collected in
the wild.
A Necessary and Sufficient Conditions for the Global Existence of Solutions to Reaction-Diffusion Equations on R N
Authors: Chung Soon-Yeong , Hwang Jaeho
Published: November 2022

A necessary-sufficient condition for the existence or nonexistence of
global solutions to the following reaction-diffusion equations { u t =
Δ u + ψ ( t ) u p , in R N ×( 0 , t ∗ ) , u ( ⋅ , 0 )= u 0 ≥ 0 , in R N
, has not been known and remained as an open problem for a few decades.
The purpose of this paper is to resolve this problem completely, even
for more general source ψ( t) f( u) as
follows: There is a global solution to the equation if and only if ∫ 0 ∞
ψ ( t ) f ( ‖ S ( t ) u 0 ‖ ∞ ) ‖ S ( t ) u 0 ‖ ∞ dt < ∞ for
some nonnegative and nontrivial u 0 ∈ C 0 ( R N ) . Here, ( S ( t ) ) t
≥ 0 is the heat semigroup on R N .
Learning to make a difference for chILD: Value creation through network collaboration and team science
Authors: McKnight Lauren , Schultz André , Vidic Nada , Palmer Elizabeth , Jaffe Adam
Published: November 2022

Addressing the recognised challenges and inequalities in providing high
quality health care for rare diseases such as children’s interstitial
lung disease (chILD) requires collaboration across institutional,
geographical, discipline, and system boundaries. The Children’s
Interstitial Lung Disease Respiratory Network of Australia and New
Zealand (chILDRANZ) is an example of a clinical network that brings
together multidisciplinary health professionals for collaboration, peer
learning, and advocacy with the goal of improving the diagnosis and
management of this group of rare and ultra-rare conditions. This
narrative review explores the multifaceted benefits arising from social
learning spaces within rare disease clinical networks by applying the
Value Creation Framework. The operation of the chILDRANZ network is used
as an example across the framework to highlight how value is generated,
realised, and transferred within such collaborative clinical and
research networks. The community of clinical practice formed in the
chILDRANZ multidisciplinary clinical peer support meetings provides a
strong example of social learning that engages with the uncertainty
inherent in rare disease diagnosis and management and pays attention to
generate new knowledge and best practice to make a difference for
children and families living with chILD. This review underscores
international calls for further investment in, and support of,
collaborative expert clinical networks for rare disease.
Nivolumab-induced acute tubular injury: a case report
Authors: YANG HUI HSIN , Chang Chia-Wen , Chen Tai-Di
Published: November 2022

A 58-year-old woman had gastric cancer treated with nivolumab. Her serum
creatinine (Cr) increased to 5.94 mg/dL post two cycles of nivolumab and
co-administered with acemetacin. A kidney biopsy showed acute tubular
injury (ATI).
Sternomanubrial reduction with plating for fully displaced sternal fracture: A case series and systematic review Written informed consent was obtained from the patient to publish this report in accordance with the journal’s patient consent policy.
Authors: Samat Sajjaad , Yelleswarapu Krishna , Zakharov Kirill
Published: November 2022

Sternal fractures are commonly due to blunt force trauma and reduction
is an invasive surgical procedure typically indicated for refractory
pain sternal instability. Retrospective literature. There were various
modalities used for treatment and fixation of the sternal
fractures.Sternal displacement fractures are traumatic injuries that may
require surgical correction.
Hybrid Architecture Based Intelligent Diagnosis Assistant for GP
Authors: Wang Ruibin , Jayathunge Kavisha , Page Rupert , Li Hailing , Zhang Jianjun , Yang Xiaosong
Published: November 2022

As the first point of contact for patients, General Practitioner (GP)
plays an important role in the National Health System. An accurate
primary diagnosis from the GP on the patient will relieve specialists’
pressure and save time from confirming the patient’s condition and doing
examinations. Because GP has broader but less specialized knowledge, the
accuracy of their diagnosis is limited. Therefore, it is imperative to
introduce an intelligent system to assist GP to make decisions. This
paper proposed a hybrid architecture, which fuses the features of words
from different representation spaces. Two data augmentation methods
(Complaint-Symptoms Integration method and Symptom Dot Separating
Method) have been proposed to integrate essential information into the
training data. Experiments demonstrate that this hybrid architecture has
good performance in the classification of 4 common neurological diseases
. Finally, this paper develops an AI diagnosis
assistant webapp which leverages the superior performance of this
architecture to help GPs complete primary diagnosis efficiently and
accurately.
VLSI Architecture of Decision-Based Adaptive Denoising Filter for Removing Salt & Pepper Noise
Authors: Sanki Pradyut Kumar
Published: November 2022

A new Decision-Based Adaptive Denoising Filter (DBADF) algorithm
& hardware architecture are proposed for restoring the digital image
that is highly corrupted with impulse noise. The proposed DBADF detects
only the corrupted pixels and that pixel is restored by the noise-free
median value or previous value based upon the noise density in the
image. The proposed DBADF uses a 3 × 3 window initially and
adaptively goes up to 7 × 7 window based on the noise
corruption more than 50% by impulse noise in the current processing
window. The proposed architecture was found to exhibit better visual
qualitative and quantitative evaluation based on PSNR, IEF, EKI, SSIM,
FOM, and error rate. The DBAMF architecture also preserves the original
information of digital image with a high density of salt & pepper
noise, when compared to many standard conventional algorithms. The
proposed architecture has been simulated using the VIRTEX7 FPGA device
and the reported maximum post place and route frequency are 149.995MHz
and the dynamic power consumption is 179mW.
Economía solidaria, procesos cognitivos con enfoque de género en Lejanías, Meta (Colombia, 2020)
Authors: Rojas Gutiérrez Saúl Eduardo , Mosquera Tapiero Mileida
Published: November 2022

Problemática: la desigualdad de género se da en todos los entornos. Para el caso de estudio, en organizaciones de economía solidaria, en el contexto de prácticas asociacionistas del ámbito rural, en el departamento del Meta, municipio de Lejanías, el cual ha sido afectado por la violencia. Objetivos: determinar la incidencia de procesos cognitivos de planificación y toma de decisiones en la economía solidaria con enfoque de género de organizaciones del municipio de Lejanías (Colombia, 2020). Materiales y métodos: estudio interdisciplinar de los procesos cognitivos a partir de la economía y la psicología de tipo probabilística, de tipo exploratorio, descriptivo y correlacional según fases, con enfoque mixto aplicado en una población de 363 asociados con muestra de 187 individuos (p = 0,5 y nivel de confianza 95%). Se aplica la teoría de juegos para análisis. Resultados: se identifican diferencias en los ingresos, las actividades agrícolas y la comercialización. Se valida la hipótesis de trabajo con categoría emergente de autogestión y la inferencia de una mejor explicación de un modelo en la submuestra hombres. A partir de esto se establece el equilibrio de Nash en estrategias mixtas (ENEM), compuesta por elementos que describen, de la mujer alta participación en planificación (MAPP), y mujer alta participación en toma de decisiones (MAPTD), en hombres alta participación en planificación (HAPP) y hombres alta participación en toma de decisiones (HAPTD); equilibrio determinado en: (1/2) MAPP+(1/2) MAPTD, (1/2) HAPP + (1/2) HAPTD. Discusión: del resultado es importante reconocer la participación de la mujer en procesos de planificación y toma de decisiones, transitando de la falencia a la oportunidad, postulando resignificaciones a partir de equilibrios gestionados en función de la reconfiguración de desigualdades. Conclusiones: de manera concluyente se presenta la economía conductual como espectro que explica las variaciones de capacidades en fuerza en trabajo familiar, específicamente en el uso de estrategias como la economía de fichas y los contratos de contingencias en espacios de planificación y toma de decisiones en función del aprovechamiento y la eficiencia.
Evolución de la producción científica sobre Servicios de Atención de Salud a Domicilio: análisis bibliométrico MEDLINE versus EMBASE
Authors: Sanz-Lorente María
Published: November 2022

Objetivo: Analizar la evolución de la producción científica sobre Servicios de Atención de Salud a Domicilio indizada en las bases de datos bibliográficas MEDLINE y Embase.
Método: Estudio descriptivo transversal. Los datos se obtuvieron de las bases de datos MEDLIEN y Embase, interrogando mediante los descriptores “Home Care Services” y “Home Care” según la base a interrogar. El periodo estudiado fue desde el inicio de la indización en esta base de datos hasta el 31 de diciembre de 2021.
Resultados: Se comprobó un modelo de regresión con muy buen ajuste al lineal creciente, siendo el coeficiente de determinación R2 = 0,91 (p < 0,001). Las tendencias observadas fueron para MEDLINE R2 = 0,94 y para Embase R2 = 0,85. La obsolescencia ofreció en MEDLINE un semiperiodo de Burton-Kebler (B-K) igual a 19 años y en Embase de 12 años. El Índice de Price fue en MEDLINE del 13,71% y en Embase del 23,68%. Se observó asociación significativa directa en la relación Embase versus MEDLINE (1 versus 0,83; p < 0,001).
Conclusiones: La producción científica presentó un adecuado incremento lineal sin alcanzar el modelo exponencial. Si bien, los resultados sobre obsolescencia fueron superiores a lo esperado. La relación de la producción científica entre las dos bases demostró asociación directa significativa.
Cambios posológicos con el fin de aumentar la adherencia a los tratamientos a través de Sistema Personalizado de Dosificación (SPD) en un paciente domiciliario: Un caso de Atención Farmacéutica Domiciliaria
Authors: Domingo-Pueyo Andrea , Campos-Martínez Beatriz
Published: November 2022

Paciente de 87 años incluido en el Servicio de Sistema Personalizado de Dosificación (SPD) a petición de su esposa. Tras seguir el Protocolo Normalizado de Trabajo de la Farmacia, se prepara la medicación del paciente en dichos dispositivos.
Ante la falta de adherencia a los tratamientos, se decide proponer al médico un cambio de posología al que accede. A partir de este momento, el paciente tiene una adherencia a los tratamientos del 100%.
El uso del SPD es una excelente herramienta para ayudar a los pacientes en el manejo de la medicación y además permitir observar la adherencia a la misma y en su defecto realizar propuestas de modificación para aumentarla. Así pues, un seguimiento multidisplinar del paciente aumenta el cumplimiento terapéutico, mejorando su calidad de vida y suponiendo un ahorro al Sistema Nacional de Salud.
Adherencia a la vacunación COVID-19 en profesionales sanitarios: revisión exploratoria
Authors: Verdú-Victoria Marina , Lopez-Pintor Elsa
Published: November 2022

Introducción: Revisar la literatura científica relacionada con la adherencia a la vacunación COVID-19 entre los profesionales sanitarios y explorar las barreras y facilitadores que inclinan la balanza hacia la aceptación o hacia la vacilación de la vacunación contra la COVID-19.
Métodos: Revisión exploratoria de los artículos recuperados en la base de datos bibliográfica MEDLINE (vía PubMed) hasta marzo de 2022. La ecuación de búsqueda se formuló mediante los descriptores “Occupational Groups”, “Vaccination”, “Coronavirus Infections”, “COVID-19 Vaccines” y “Treatment Adherence and Compliance”, utilizando también los Entry Terms relacionados y los filtros: «Humans» y «Adult: 19+ years». Para conocer el nivel de evidencia y su grado de recomendación se usaron las recomendaciones SIGN.
Resultados: De las 135 referencias recuperadas, tras aplicar los criterios de inclusión y exclusión, se seleccionaron 27 artículos: 26 estudios descriptivos transversales y 1 estudio cualitativo. En 17 (62,96%) artículos se midió la aceptación, cuyos datos recopilados mostraron una aceptación moderada tomando como valor conforme un 70% de aceptación para frenar la pandemia por COVID-19. Y en 21 (77,78%) artículos que cuantificaron la vacilación se observaron valores de reticencia elevados, pero que, a largo plazo, seguían una tendencia a la baja. Además, se recopilaron las principales barreras para la vacunación contra la COVID-19, las cuales eran: preocupaciones acerca de la seguridad, eficacia, rápido desarrollo de las vacunas contra la COVID-19 y falta de información y confianza en ellas y los principales facilitadores: sexo masculino, haber sido vacunado anteriormente contra la gripe, ser médico, enfermero o trabajos afines en los que se tiene contacto con los pacientes.
Conclusiones: Se recomienda mejorar la información, comunicación y la formación de los profesionales sanitarios debido a ser los asesores más fiables y los que más influyen en las decisiones de vacunación de la población general.
Association between Non Alcoholic Fatty Liver Disease and Polycystic Ovarian Syndrome: A Systematic Review
Authors: MU Ashraf
Published: November 2022

Nonalcoholic fatty liver disease (NAFLD), has emerged as one of the most common causes of chronic liver disease and is increasingly being attributed to cirrhosis in a large number of patients. NAFLD is characterized by accumulation of fat in hepatocytes without a significant history of alcohol consumption. Since the prevalence of NAFLD is markedly increased in obesity, type 2 diabetes mellitus and dyslipidemia, the role of insulin resistance in the pathogenesis of this disease has been studied and a strong association has been found between insulin resistance and NAFLD. It is now established that NAFLD is the hepatic component of the metabolic syndrome. On the other hand, polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies in premenopausal women. Insulin resistance which is the hallmark of metabolic syndrome, has also been documented in about 50% to 80% of women with PCOS. Over the past few years evidence has accumulated showing an association between nonalcoholic fatty liver disease and polycystic ovary syndrome. The pathophysiologic link and the clinical significance of this relationship are still not very well understood, however, there is sufficient data to support the same. We present here a systematic review of studies which have evaluated this association and have underlined the clinical implications of this co-existence
Adjunctive and Transformed Immunity: Histiocytic & Dendritic Cell Neoplasm
Authors: A Bajaji
Published: November 2022

Exceptional malignancies of the lymph nodes or soft tissue comprising of < 1% of the tumour incidence are the Histiocytic and Dendritic cell neoplasm. A definitive appearance/biology/ Hematology/ histopathology / exclusive therapeutic options describe the condition. Morphology and Immune reactive appraisal may be mandated to distinguish the neoplasm. Preface: Histiocytic and Dendritic neoplasms are infrequent disorders which incriminate the accessory immune system or mesenchymal cells. Subject to the origin of the neoplasm, from the bone marrow precursors or the mesenchye, the lesions may be categorized as. i) The Histiocytic sarcoma (HS), Langerhans cell histiocytosis(LCH) and the Inter-digitizing dendritic cell sarcoma (IDCS) may commence from the bone marrow precursors and ii) The Follicular dendritic cell sarcoma (FDCS), Intermediate dendritic cell sarcoma (INDCS), Fibroblastic reticular cell tumour (FRCT) and Disseminated juvenile xanthogranuloma(DJX) may emanate from the Stromal or the mesenchymal cell. Divergent differentiation of the bone marrow precursors is a usual manifestation, though hybrid or trans-differentiating malignant lymphoid clones may also materialize in Inter-digitizing dendritic cell sarcoma (IDCS), Histolytic sarcoma (HS) and Follicular dendritic cell sarcoma (FDCS). Together, Histolytic and Dendritic cell tumors constitute < 1% of the lymph node or soft tissue neoplasm. The tumors may be misinterpreted as Non Hodgkin’s Lymphoma or adjunct Lymph-Proliferative disorders. The rare malignancies may be a challenge to discern and medicate. Debatable Histolytic and Dendritic neoplasm mandate a referral. The disorder may be ascertained by the combined analysis on Histology and Immunehistochemistry
Gluten Related Disorders - A Clinical and Nutritional Approach
Authors: G Choudhuri
Published: November 2022

With the urbanization and development of mankind, digestive disorders due to wheat or its constituent gluten has seen increased prevalence globally. Celiac disease (CD), non-celiac gluten sensitivity (NCGS) and Wheat Allergy are all conditions in which the primary treatment is avoidance of specific dietary components. There are certain overlaps and often common misconceptions associated with these three conditions. Following a wheat or gluten free diet can be extremely difficult and overwhelming for an individual as well as having an impact on their nutritional status, growth and quality of life. Currently there is a lack of clarity in terms of global standard dilations for the management of all three of the named conditions. The aim of this paper is to provide an update on different gluten related disorders, their nutritional management and to provide practical guidance on implementing the specific dietary changes.