bluuebunny/crossref_metadata_2025 huggingface dataset:
Coxiella burnetii: A Brief Summary of the Last Five Years of Its Presence in the Abruzzo and Molise Regions in Italy
Authors: Alessiani Alessandra , Di Domenico Marco , Averaimo Daniela , Pompilii Cinzia , Rulli Marco , Cocco Antonio , Lomellini Laura , Coccaro Antonio , Cantelmi Maria Chiara , Merola Carmine , Tieri Elga Ersilia , Romeo Gianfranco , Secondini Barbara , Marfoglia Cristina , Di Teodoro Giovanni , Petrini Antonio
Published: August 2024

Coxiella burnetii is the causative agent of Q fever. The main reservoirs for this bacterium, which can lead to human infection, in our region are typically cattle, goats, and sheep. In animals, C. burnetii infection is often detected due to reproductive problems. European Member States are required to report confirmed cases annually, but the lack of uniform reporting methods makes the data rather inconsistent. The Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise is involved in official controls to identify the causes of abortions, monitor suspected or positive herds, evaluate suspected infections in pets and humans, monitor the spread in wildlife, etc. In this paper, we summarize the presence of C. burnetii over the last five years (2019–2023). Additionally, a detailed overview of C. burnetii infection in wild and domestic animals is provided. Five hundred sixty animals—including cattle; goats; sheep; wild animals, such as deer, boars, wolves, roe deer, owls, and otters; buffalo; dogs; horses; cats; and a donkey—and six human samples were tested by real-time PCR on the transposase gene IS1111 to detect C. burnetii. The MST profile was identified in some of the samples. Outbreaks of C. burnetii occurred in four herds. In one of them, it was possible to follow the outbreak from inception to eradication by evaluating the effect of vaccination on real-time PCR Ct values. A total of 116 animals tested positive for C. burnetii, including 73 goats, 42 sheep, and one bovine. None of the other samples tested positive. The strains for which the ST was performed were identified as ST79, a strain that has been present in the area for more than ten years. The effect of vaccination on the reduction of positive samples and the variation of real-time PCR Ct values was evaluated in strict correlation.
The Effects of Intra-Articular Triamcinolone and Autologous Protein Solution on Metabolic Parameters in Horses
Authors: Page Allen E. , Johnson Mackenzie , Parker Jordan L. , Jacob Olivia , Poston Rachel , Adams Amanda A. , Adam Emma N.
Published: August 2024

Intra-articular corticosteroids are a popular treatment choice for joint-associated pain and inflammation in horses despite recent work on the metabolic effects of these drugs. The goal of this project was to compare metabolic effects between intra-articular (IA) triamcinolone acetonide (TA) and an autologous protein solution (APS). Five mixed-breed geldings (4–9 years) were utilized for this project. Three identical and consecutive 28-day treatment blocks were used, with metacarpophalangeal IA treatments consisting of equal volumes of saline, a commercially available APS, or 9 mg of TA. Regular plasma and serum samples were collected for ACTH, cortisol, glucose, insulin, and thyroid hormone analysis, in addition to thyrotropin-releasing hormone (TRH) and oral sugar tests (OSTs). Significant treatment effects of IA TA were present at 48 h post-injection in both the TRH and the OST. There was also significant suppression by IA TA of baseline ACTH and cortisol between 2 h and 96 h post-treatment, hyperglycemia between 12 h and 48 h, and hyperinsulinemia at 32 h post-treatment. There were no treatment effects with respect to any measured thyroid hormones, nor were there any significant treatment effects of APS noted. Results suggest at least 2 days and up to 7 days should elapse between a single 9 mg IA TA treatment and OST and/or TRH testing. This study found that TA exhibits significant effects on ACTH, cortisol, glucose, and insulin, while the APS does not.
Phytochemical Evaluation of Lepidium meyenii, Trigonella foenum-graecum, Spirulina platensis, and Tribulus arabica, and Their Potential Effect on Monosodium Glutamate Induced Male Reproductive Dysfunction in Adult Wistar Rats
Authors: Shehab Naglaa Gamil , Omolaoye Temidayo S. , Du Plessis Stefan S. , Rawat Surendra Singh , Naidoo Nerissa , Abushawish Kholoud Y. , Ahmed Ayat , Alaa Baraa , Ihsan Heba , Abdelhalim Manar , Ayman Mariam , El Nebrisi Eslam
Published: August 2024

Monosodium glutamate (MSG), a sodium salt derived from glutamic acid, is widely used in commercial food products to improve taste, quality, and preservation. However, its consumption may have detrimental effects on male reproductive function. Nevertheless, plant extracts, such as Lepidium meyenii (Maca), Trigonella foenum-graecum (Fenugreek), Spirulina platensis (Spirulina), and Tribulus arabica (Tribulus), may ameliorate these adverse effects. To this effect, the phytochemical properties of Lepidium meyenii, Trigonella foenum-graecum, Spirulina platensis, and Tribulus arabica were assessed, and their potential impact on MSG-induced impairment of reproductive parameters was examined. The phytochemical composition (steroids, terpenes, phenols, flavonoids) of the plants was profiled through spectrophotometry and the antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Thirty-six male Wistar rats were divided into six groups at random: a control group receiving distilled water, and five experimental groups (MSG, Maca, Fenugreek, Spirulina, and Tribulus) receiving 900 mg/kg/day of MSG dissolved in water for 45 days. Subsequently, the animals in the experimental groups were administered 500 mg/kg/day of the respective plant extract via oral gavage for an additional 35 days, while the MSG group continued to receive water only. Following the treatment period, the animals were sacrificed, and their reproductive tract organs were collected, weighed, and subjected to further analysis. Phytochemical analysis revealed the presence of diverse bioactive elements in the plant extracts, including phenolic and flavonoid compounds. Exposure to MSG negatively impacted total and progressive sperm motility, which was ameliorated by Lepidium meyenii treatment. Sperm morphology showed no significant differences among groups. Treatment of the phytochemical agents diminished histomorphometric alternations of the testicular length, germinal epithelium height, and number of cells in seminiferous tubules, which were caused by the initial administration of MSG. Testosterone and LH levels were reduced in the MSG group but improved in extract-treated groups. The study suggests Lepidium meyenii as a potential remedy for reproductive dysfunction. However, further investigation into its mechanisms and human safety and efficacy is warranted.
Impact of Music Selection on Motivation and Performance during Cardiopulmonary Exercise Testing
Authors: Labudović Dragana , Stojiljković Stanimir , Orlić Ana , Matić Milan , Uzunović Slavoljub , Bubanj Saša , Dobrescu Tatiana , Macura Marija , Popović Dejana
Published: August 2024

Background: The aim of this study was to examine the effect of applying synchronized music and appropriate music selection on motivation for exercise and achieving better results in individuals of different fitness levels. Methods: The study included a total of 20 female participants, who for certain analyses were divided into two groups with slightly different levels of aerobic fitness: students from the Faculty of Sport and Physical Education (n = 10, age 23.0 ± 2.8), and middle-aged adult women exercising recreationally (n = 10, age 38.3 ± 11.6). Cardiopulmonary exercise testing (CPET) was conducted using a treadmill and gas analysis equipment, and motivational music qualities were assessed using the BMRI-2 questionnaire. The procedure included an initial maximal CPET test, echocardiography, and spirometric tests, followed by an interview to select preferred music tracks. A second CPET test was then performed with the chosen motivational music. The Borg Rating of Perceived Exertion (RPE) scale was used in both tests. Results: The internal consistency of the questionnaire was confirmed with a Cronbach’s Alpha of 0.982. The synchronized motivational music significantly improved cardiopulmonary parameters such as peak oxygen consumption (peak VO2), oxygen consumption (VO2) at the second ventilatory threshold (VT2), peak heart rate (peak HR), test duration, and reduced perceived exertion (RPE) at the beginning of the test and at the intensity level corresponding to the VT2. Negligible differences were noted between students and recreational athletes, so it can be assumed that music had an equal impact on these two groups of subjects. Conclusions: The study concluded that synchronous motivational music significantly enhances cardiopulmonary performance and reduces perceived fatigue during physical exertion by serving as a key motivational element and facilitating more economical movement.
Technique Variables Associated with Fast Bowling Performance: A Systematic-Narrative Review
Authors: Bhandurge Shruti , Alway Peter , Allen Sam , Blenkinsop Glen , King Mark
Published: August 2024

The performance characteristics of fast bowling include high ball release speed, that reduces the shot execution time of the batter, accuracy, ensuring the ball follows the desired trajectory, and deception. This can lead the batter to misjudge the ball speed or path. Previous research has utilised a variety of biomechanical methodologies in order to further understand fast bowling techniques. The aim of this study was to systematically review biomechanical literature related to cricket fast bowling performance and narratively synthesise findings to provide a comprehensive summary of key performance characteristics. The articles were finalised according to Preferred Reporting Items for Systematic Reviews (PRISMA) guidelines. PubMed/MEDLINE, Scopus, CINAHL, SPORT Discus and Web of Science databases were searched and assessed by title, abstract, full-text and a bibliography assessment of included full-text articles. The search strategy included keywords of cricket fast bowling, biomechanics and performance analysis. A validated scale was developed to evaluate the quality of studies and risk of bias. Across the 15 studies finalised for the review (from 2000--2022), there was consensus in some of the characteristics reported to be associated with ball release speed; however, there was disagreement or limited evidence for others. A faster run-up, efficient and sequential transfer of linear to angular momentum, quick deceleration during the delivery stride with an extended front knee from front foot contact to ball release and delaying the bowling arm leading to ball release were the major techniques variables consensually associated with ball release speed. The lack of standardisation of cohort ability and protocols may have contributed to contradictory findings across studies.
Heat Generated during Dental Implant Placement: A Scoping Review
Authors: Cimini Valerio , Perez Alexandre , Lombardi Tommaso , Felice Roberto Di
Published: August 2024

Background: Osseointegration is fundamental to achieving successful implant therapy in dentistry. However, the heat generated during implant placement emerges as a critical factor predisposing to implant failure. Objective: This study aimed to analyze the different factors related to heat generation during implant placement, offering insights to clinicians in their daily clinical practice. Methods: Utilizing the PubMed, Web of Science, and Embase databases, we conducted an electronic search for articles published between January 2013 and December 2023. The analysis focused on several factors including bone type, drill shape, drill speed, drill material, drilling force, osteotomy depth, drill load, drilling technique (intermittent or continuous), presence of a surgical guide, irrigation methods, drill wear, and preparation tools available. Results: Initially, 2525 records were identified. After applying the inclusion and exclusion criteria and full-text assessment, 93 articles were included in this scoping review. Additionally, some articles published before 2013 were incorporated in the bibliography to ensure completeness of the review. Conclusions: Heat generation during implant placement arises from a complex interplay of multiple factors. While irrigation and bone hardness appear to be crucial determinants of heat generation during the osteotomy phase, the involvement of other factors remains less clear. Further studies are needed to better understand the precise contribution of these factors towards increasing temperature at the implant site.
A Survey on Surface Defect Inspection Based on Generative Models in Manufacturing
Authors: He Yu , Li Shuai , Wen Xin , Xu Jing
Published: August 2024

Surface defect inspection based on deep learning has demonstrated outstanding performance in improving detection accuracy and model generalization. However, the small scale of defect datasets always limits the application of deep models in industry. Generative models can obtain realistic samples in a very cheap way, which can effectively solve this problem and thus has received widespread attention in recent years. This paper provides a comprehensive analysis and summary of the current studies of surface defect inspection methods proposed between 2022 and 2024. First, according to the use of generative models, these methods are classified into four categories: Variational Auto-Encoders (VAEs), Generative Adversarial Networks (GANs), Diffusion Models (DMs), and multi-models. Second, the research status of surface defect inspection based on generative models in recent years is discussed from four aspects: sample generation, detection objective, inspection task, and learning model. Then, the public datasets and evaluation metrics that are commonly used for surface defect inspection are discussed, and a comparative evaluation of defect inspection methods based on generative models is provided. Finally, this study discusses the existing challenges for the defect inspection methods based on generative models, providing insights for future research.
Survival and Growth of Asellus aquaticus on Different Food Sources from Drinking Water Distribution Systems
Authors: van Bel Nikki , van Lieverloo J. , Verschoor Antonie , Pap-Veldhuizen Leonie , Hijnen Wim , Peeters Edwin , Wunderer Julia
Published: August 2024

Invertebrates, including Asellidae, are part of the natural ecosystem of the drinking water distribution system (DWDS) and are known to cause a nuisance to consumers. In addition, recently, the potential role of the species Asellus aquaticus (L. 1758) in the regrowth of Aeromonas bacteria was published. Aeromonas is included in the Dutch drinking water guidelines as a process parameter, and the guideline values are regularly exceeded. Although neither A. aquaticus nor Aeromonas is associated with health risks, the Evides drinking water utility shows a strong interest in the possible reasons for these exceedances and possible control measures. In surface waters, Asellidae feed mainly on decaying leaves that are abundantly present. These food sources are not present in the DWDS. Therefore, we determined suitable food sources for A. aquaticus in the DWDS. Laboratory experiments show that A. aquaticus individuals survive on biofilm on pipe wall material and loose deposits (sediments) collected from DWDS. Growth and survival rates on these loose deposits were even higher than on the positive control (decaying leaves). As the basis of these loose deposits is inorganic (iron deposits, sand, and pipe particles), the organic matter (living and decaying bacteria, protozoans, fungi, and invertebrates) must be their substrate. These experiments validate hypotheses that Asellidae can grow and survive on organic matter in deposits in DWDS.
Linearized Stability Analysis of Nonlinear Delay Differential Equations with Impulses
Authors: Bachar Mostafa
Published: August 2024

This paper explores the linearized stability of nonlinear delay differential equations (DDEs) with impulses. The classical results on the existence of periodic solutions are extended from ordinary differential equations (ODEs) to DDEs with impulses. Furthermore, the classical results of linearized stability for nonlinear semigroups are generalized to periodic DDEs with impulses. A significant challenge arises from the need for a discontinuous initial function to obtain periodic solutions. To address this, first-kind discontinuous spaces R([a,b],Rn) are introduced for defining DDEs with impulses, providing key existence and uniqueness results. This study also establishes linear stability results by linearizing the Poincaré operator for DDEs with impulses. Additionally, the stability properties of equilibrium solutions for these equations are analyzed, highlighting their importance due to the wide range of applications in various scientific fields.
FinSoSent: Advancing Financial Market Sentiment Analysis through Pretrained Large Language Models
Authors: Delgadillo Josiel , Kinyua Johnson , Mutigwe Charles
Published: August 2024

Predicting the directions of financial markets has been performed using a variety of approaches, and the large volume of unstructured data generated by traders and other stakeholders on social media microblog platforms provides unique opportunities for analyzing financial markets using additional perspectives. Pretrained large language models (LLMs) have demonstrated very good performance on a variety of sentiment analysis tasks in different domains. However, it is known that sentiment analysis is a very domain-dependent NLP task that requires knowledge of the domain ontology, and this is particularly the case with the financial domain, which uses its own unique vocabulary. Recent developments in NLP and deep learning including LLMs have made it possible to generate actionable financial sentiments using multiple sources including financial news, company fundamentals, technical indicators, as well social media microblogs posted on platforms such as StockTwits and X (formerly Twitter). We developed a financial social media sentiment analyzer (FinSoSent), which is a domain-specific large language model for the financial domain that was pretrained on financial news articles and fine-tuned and tested using several financial social media corpora. We conducted a large number of experiments using different learning rates, epochs, and batch sizes to yield the best performing model. Our model outperforms current state-of-the-art FSA models based on over 860 experiments, demonstrating the efficacy and effectiveness of FinSoSent. We also conducted experiments using ensemble models comprising FinSoSent and the other current state-of-the-art FSA models used in this research, and a slight performance improvement was obtained based on majority voting. Based on the results obtained across all models in these experiments, the significance of this study is that it highlights the fact that, despite the recent advances of LLMs, sentiment analysis even in domain-specific contexts remains a difficult research problem.
Comprehensive Environmental Impact Analysis of Dry Processing Methods for Specialty Coffee Beans in Bondowoso, Indonesia Using Life Cycle Assessment
Authors: Irawan Andik , Rabemanolontsoa Harifara , McLellan Benjamin C.
Published: August 2024

Smallholders play a key role in specialty coffee production. Implementing industrial ecology coffee (IEC) practices is crucial for sustainable coffee production (SCP), aiming to add value, achieve zero waste, and respect the environment. For that purpose, this study used life cycle assessment (LCA) to assess the environmental impact of coffee production, specifically focusing on the global warming potential (GWP) of dry methods (DMs). Data were collected from pilot plant operations in Bondowoso, Indonesia, covering the process from cherry beans (CBs) to coffee powder (CP). A unique aspect of this study is assessing the impact of the DMs: Natural, Anaerobic, Hydro honey, Lactic, and Carbonic Maceration, which were often overlooked in previous research. Observations and experimental results served as primary data for input calculations in LCA. As a result, it was found that for the studied DMs, inputting 150 kg per batch of CBs produced approximately 22.4–22.8 kg of CP. The LCA revealed that for one kg of CP produced by the DMs, GWP ranged from 0.676 kg to 1.168 kg of CO2-eq, with Natural being the least polluting and Lactic having the highest environmental impact. This study also suggests potential improvements in by-products for novel food and fuel applications.
Differential Cytotoxicity of Flavored E-Liquids with and without Nicotine on Neonatal Human Melanocytes from Lightly and Darkly Pigmented Donors: A Preliminary Report
Authors: Goenka Shilpi
Published: August 2024

The increasing use of e-cigarettes (ECs) has raised public health concerns due to the observed cytotoxic effects in both in vitro and in vivo studies. Infants and young children, being particularly vulnerable groups, exhibit heightened susceptibility to potential hazards arising from maternal use of ECs, as well as exposure to second-hand and third-hand aerosols emitted by ECs. Melanocytes are neural-crest-derived cells that regulate multiple biological functions. Melanocyte death, triggered by chemical exposure, is a known etiological cause of pigmentation abnormalities and neurodevelopmental disorders. Prior reports have demonstrated nicotine-induced differential cytotoxicity to neonatal human melanocytes derived from lightly pigmented (LP) and darkly pigmented (DP) donors. We recently reported that the vehicle base propylene glycol (PG) in e-liquid can alter the functions of LP melanocytes. However, to date, the effects of e-liquid flavors on LP and DP cells remain unexplored. Hence, in this preliminary study, a panel of twenty EC refill liquids comprising ten popular flavors (strawberry, grape, banana, vanilla, butterscotch, cinnamon, menthol, chocolate, cola, and tobacco), where each flavored e-liquid contained either 0 or 18 mg/mL nicotine, was examined for in vitro cytotoxicity to neonatal human melanocytes derived from LP and DP donors. Our results reveal that of the ten flavors, five (menthol, cinnamon, vanilla, tobacco, and banana) were highly cytotoxic, with their half-maximal inhibitory concentration (IC50) values within the tested concentration ranges. Moreover, the cytotoxicity of the specific flavors menthol, cinnamon, and vanilla was enhanced in the presence of nicotine, indicative of interactive effects, with nicotine and flavor contributing to greater melanocyte injury. The cytotoxicity of menthol (both with and without nicotine) and cinnamon (without nicotine) e-liquids was found to be higher in LP cells as compared to DP cells. In contrast, nicotine-containing vanilla e-liquid induced higher cytotoxicity in DP cells than LP cells. Only three flavors, cola (without nicotine), strawberry (without nicotine), and chocolate (without nicotine), were non-cytotoxic to both LP and DP cells. The findings that popular flavors in e-liquids induced moderate to high degree of melanocytotoxicity even in the absence of nicotine suggests that ECs are not harmless. This information may assist EC users identify particular flavors in refill liquids that may be detrimental to melanocytes. A first-screen identification of flavors in e-liquids that show a racial/ethnicity dependence can provide a baseline to identify cytotoxicity concentration ranges for popular flavors and help inform the regulatory guidelines for EC toxicity to young children and youth.
DCTPP1 Expression as a Predictor of Chemotherapy Response in Luminal A Breast Cancer Patients
Authors: Muñoz Juan P. , Soto-Jiménez Diego , Calaf Gloria M.
Published: August 2024

Breast cancer (BRCA) remains a significant global health challenge due to its prevalence and lethality, exacerbated by the development of resistance to conventional therapies. Therefore, understanding the molecular mechanisms underpinning chemoresistance is crucial for improving therapeutic outcomes. Human deoxycytidine triphosphate pyrophosphatase 1 (DCTPP1) has emerged as a key player in various cancers, including BRCA. DCTPP1, involved in nucleotide metabolism and maintenance of genomic stability, has been linked to cancer cell proliferation, survival, and drug resistance. This study evaluates the role of DCTPP1 in BRCA prognosis and chemotherapy response. Data from the Cancer Genome Atlas Program (TCGA), Genotype-Tissue Expression (GTEx), and Gene Expression Omnibus (GEO) repositories, analyzed using GEPIA and Kaplan–Meier Plotter, indicate that high DCTPP1 expression correlates with poorer overall survival and increased resistance to chemotherapy in BRCA patients. Further analysis reveals that DCTPP1 gene expression is up-regulated in non-responders to chemotherapy, particularly in estrogen receptor (ER)-positive, luminal A subtype patients, with significant predictive power. Additionally, in vitro studies show that DCTPP1 gene expression increases in response to 5-fluorouracil and doxorubicin treatments in luminal A BRCA cell lines, suggesting a hypothetical role in chemoresistance. These findings highlight DCTPP1 as a potential biomarker for predicting chemotherapy response and as a therapeutic target to enhance chemotherapy efficacy in BRCA patients.
Effects of Exhaustive Exercise on Adiponectin and High-Molecular-Weight Oligomer Levels in Male Amateur Athletes
Authors: Mallardo Marta , Tommasini Ester , Missaglia Sara , Pecci Claudio , Rampinini Ermanno , Bosio Andrea , Morelli Andrea , Daniele Aurora , Nigro Ersilia , Tavian Daniela
Published: August 2024

Physical activity promotes metabolic health and prevents lifestyle-related diseases. Adiponectin is specifically produced by adipose tissue and comes in three forms, differing in terms of weight: trimers (LMW), hexamers (MMW), and high-molecular-weight (HMW) oligomers. The oligomers are associated with the beneficial effects of adiponectin. In this study, we aimed to investigate the impact of a single bout of exhaustive exercise on adiponectin expression in 25 male amateur athletes, divided into two groups, one comprising young adults (YAs) (n = 15), and the other comprising middle-aged adults (MAs) (n = 10). Body fat was estimated through skinfold thickness. Adiponectin levels were assessed at baseline and at 15 min and 24 h post-exercise, while HMW oligomer levels were evaluated at baseline and at 24 h post-exercise. We observed a significant increase in total adiponectin at both 15 min and 24 h after exercise, with there being a more evident effect among the YA subjects. HMW oligomers also increased significantly after exercise both in the total sample and among the YA subjects, but this was not the case in the MA subjects. The increase in adiponectin levels was significantly associated with Powerpeak. Furthermore, a significant inverse correlation was found between basal adiponectin and VO2peak and Powerpeak. In conclusion, a single bout of exhaustive exercise can rapidly and significantly enhance the basal circulating adiponectin concentration, which seems to be negatively associated with maximal aerobic capacity.
Insecure Attachment, Oxytocinergic System and C-Tactile Fibers: An Integrative and Translational Pathophysiological Model of Fibromyalgia and Central Sensitivity Syndromes
Authors: Bruti Gianluca , Foggetti Paola
Published: August 2024

Although the pathophysiology of fibromyalgia syndrome has been better understood in recent decades, a unified model of its pathogenesis and an effective therapeutic approach are still far from being realized. The main aim of this article will be to delve into the fundamental mechanisms of the pathophysiology of fibromyalgia conceptualized as stress intolerance syndrome. Using the biopsychosocial model of chronic pain syndromes, we will describe the potential role of the attachment system, C-tactile fibers, and oxytocinergic system dysfunction in the pathophysiology of fibromyalgia syndrome and other central sensitivity syndromes. At the end of the article, the therapeutic implications of this new global and translational pathophysiological model will be briefly discussed.
Molecular Insight into the Response of Lactic Acid Bacteria to Bile Acids
Authors: Moreno Caren N. , Gomez Jorge N. , Taranto María P. , Ledesma Ana E. , Bustos Ana Y.
Published: August 2024

Bile acids (BAs) are the main endogenous modulators of the composition and metabolic activity of the intestinal microbiota. In the present work, the effect of conjugated (glycodeoxycholic, glycocholic, taurodeoxycholic, taurocholic acids) and free BAs [cholic acid (CA) and deoxycholic acid (DCA)] on the survival, biological molecules, and structural and surface properties of two potential probiotic lactic acid bacteria (LAB) was evaluated. For this, viability assays, Raman spectroscopy, scanning electron microscopy (SEM), and zeta potential (ZP) measurements were employed. Our results evidenced that free BAs were more toxic than conjugates, with CA being significantly more harmful than deoxycholic acid (DCA). RAMAN studies show that BAs modify the bands corresponding to proteins, lipids, carbohydrates, and DNA. SEM showed that BAs cause surface distortions with depressions and fold formation, as well as incomplete cell division. DCA was the one that least altered the ZP of bacteria when compared to CA and taurodeoxycholic acid, with gradual changes towards more positive values. In general, the magnitude of these effects was different according to the BA and its concentration, being more evident in the presence of CA, even at low concentrations, which would explain its greater inhibitory effect. This work provides solid evidence on the effects of BAs on LAB that will allow for the development of strategies by which to modulate the composition of the microbiota positively.
Semantic Segmentation of Heavy Construction Equipment Based on Point Cloud Data
Authors: Park Suyeul , Kim Seok
Published: August 2024

Most of the currently developed 3D point cloud data-based object recognition algorithms have been designed for small indoor objects, posing challenges when applied to large-scale 3D point cloud data in outdoor construction sites. To address this issue, this research selected four high-performance deep learning-based semantic segmentation algorithms for large-scale 3D point cloud data: Rand-LA-Net, KPConv Rigid, KPConv Deformable, and SCF-Net. These algorithms were trained and validated using 3D digital maps of earthwork sites to build semantic segmentation models, and their performance was tested and evaluated. The results of this research represent the first application of 3D semantic segmentation algorithms to large-scale 3D digital maps of earthwork sites. It was experimentally confirmed that object recognition technology can be implemented in the construction industry using 3D digital maps composed of large-scale 3D point cloud data.
Retrospective Cohort Study on the Impact of Travel Distance on Late-Stage Oral Cancer Treatment and Outcomes: An NCDB Analysis
Authors: Harris Courtney S. , Groman Adrienne , Sigurdson S. Lynn , Magner William J. , Singh Anurag K. , Gupta Vishal
Published: August 2024

The National Comprehensive Cancer Network guidelines provide evidence-based consensus for optimal individual site- and stage-specific treatments. This is a cohort study of 11,121 late-stage oral cancer patients in the National Cancer Database from 2010 to 2016. We hypothesized that patient travel distance may affect treatment choices and impact outcome. We split travel distance (miles) into quartiles (D1–4) and assessed treatment choices, type of facility, and survival outcome in relation to distance traveled. Univariate and multivariate analyses addressed contributions of specific variables. White patients were most likely to travel farthest (D4) for treatment compared to Black patients (D1). Urban area patients traveled shorter distances than those from rural areas. Greater travel distance was associated with patients undergoing surgical-based therapies and treatment at academic centers. Patients in D1 had the lowest median survival of all distance quartiles. Surgery-based multimodality treatment (surgery and radiation) had a median survival significantly greater than for non-surgical therapy. Several factors including travel distance and treatment facility were associated with survival outcomes for late-stage oral cavity cancers. Consideration of these factors may help improve the outcome for this patient population.
Imaging Biomarkers of Oral Dysplasia and Carcinoma Measured with In Vivo Endoscopic Optical Coherence Tomography
Authors: Malone Jeanie , Hill Chloe , Tanskanen Adrian , Liu Kelly , Ng Samson , MacAulay Calum , Poh Catherine F. , Lane Pierre M.
Published: August 2024

Optical coherence tomography is a noninvasive imaging technique that provides three-dimensional visualization of subsurface tissue structures. OCT has been proposed and explored in the literature as a tool to assess oral cancer status, select biopsy sites, or identify surgical margins. Our endoscopic OCT device can generate widefield (centimeters long) imaging of lesions at any location in the oral cavity—but it is challenging for raters to quantitatively assess and score large volumes of data. Leveraging a previously developed epithelial segmentation network, this work develops quantifiable biomarkers that provide direct measurements of tissue properties in three dimensions. We hypothesize that features related to morphology, tissue attenuation, and contrast between tissue layers will be able to provide a quantitative assessment of disease status (dysplasia through carcinoma). This work retrospectively assesses seven biomarkers on a lesion-contralateral matched OCT dataset of the lateral and ventral tongue (40 patients, 70 sites). Epithelial depth and loss of epithelial–stromal boundary visualization provide the strongest discrimination between disease states. The stroma optical attenuation coefficient provides a distinction between benign lesions from dysplasia and carcinoma. The stratification biomarkers visualize subsurface changes, which provides potential for future utility in biopsy site selection or treatment margin delineation.
Improving Rutin Biotransformation Efficiency of α-L-Rhamnosidase from Bacteroides thetaiotaomicron VPI-5482 via Targeted Mutagenesis Focused on General Acid Motif
Authors: Li Bin-Chun , Li Xue , Peng Bo , Wu Bingbing , Li Xinfeng , Ding Guo-Bin
Published: August 2024

α-L-Rhamnosidases with desirable activity and thermostability profiles could be used for the biocatalytic production of the flavonoid glucoside isoquercetin from natural rutin for functional food. Herein, to improve the catalytic activity of GH78 α-L-rhamnosidase BtRha78A from Bacteroides thetaiotaomicron VPI-5482, a list of residues located at the conserved general acid motif were selected for targeted mutagenesis by the sequence alignment of BtRha78A with homologous α-L-rhamnosidases. Ala-scanning mutagenesis and site-directed mutagenesis based on sequence alignment were performed, and the relative activity on rutin was evaluated. Furthermore, the reaction time curves and enzyme kinetics of better mutants were determined. The results indicate that the conversion rates of mutants V338A, V338I, S340A, and G341A were increased by 21.3%, 20.1%, 13.2%, and 1.6%, respectively, compared with the wild type when using whole-cell biotransformation. Moreover, the catalytic efficiency kcat/KM value of mutant V338A was 1.3-fold higher than that of the wild type. The best mutant, V338A, was employed for the enzymatic preparation of isoquercetin via the biotransformation of rutin at a concentration of 2 mM, and 1.80 g of isoquercetin was obtained. The identification of the best mutant V338A lays the foundation for the efficient preparation of isoquercetin via the biotransformation of rutin, which in turn provides theoretical guidance for its large-scale production.
3D-Printed Conformal Thin Film Thermocouple Arrays for Distributed High-Temperature Measurements
Authors: Liu Jun , Xu Lida , Zhou Xiong , Zhao Fuxin , Wang Yusen , Wang Siqi , Lv Wenlong , Sun Daoheng , Chen Qinnan
Published: August 2024

Conformal thin film sensing represents a cutting-edge technology capable of precisely measuring complex surface temperature fields under extreme conditions. However, fabricating high-temperature-resistant conformal thin film thermocouple arrays remains challenging. This study reports a method for manufacturing conformal thin film thermocouple arrays on metal spherical surfaces using a printable paste composed of silicates and Ag. Specifically, the use of silicate glass phases enhances the high-temperature performance of the silver printable paste, enabling the silver ink coatings to withstand temperatures up to 947 °C and survive over 25 h at 900 °C. The thermocouples, connected to Pt thin films, exhibited a Seebeck coefficient of approximately 17 μV/°C. As a proof of concept, an array of six Ag/Pt thin film thermocouples was successfully fabricated on a metal spherical surface. Compared to traditional wire-type thermocouples, the conformal thin film thermocouple arrays more accurately reflect temperature variations at different points on a spherical surface. The Ag/Pt conformal thin film thermocouple arrays hold promise for monitoring temperature fields in harsh environments, such as aerospace and nuclear energy applications.
High-Temperature Mechanical and Tribological Performance of W-DLC Coating with Cr interlayer on X40CrMoV5-1 Hot Work Tool Steel
Authors: Hagarová Mária , Baranová Gabriela , Heželová Mária , Truchlý Martin , Vojtko Marek , Petruš Ondrej , Csík Dávid
Published: August 2024

Diamond-like carbon (DLC) coatings enhance tool wear resistance across various temperatures. The sp3/sp2 bond ratio within DLC significantly impacts its properties and thermal stability. Elevated temperatures can alter DLC’s structure, while metallic elements and interlayers like chromium can modify its microstructure and performance. To evaluate the potential of W-DLC coatings with a chromium interlayer on 40CrMoV5-1 hot work tool steel under elevated temperatures, mechanical and tribological properties were assessed at room temperature and at temperatures of 100, 200, 300, 400, and 500 °C. Nanoindentation revealed a maximum hardness of 14.1 ± 1.3 GPa for the coating deposited at room temperature, attributed to a high sp3 content confirmed by Raman spectroscopy. Hardness decreased to 9.3 ± 1.0 GPa at 400 °C due to graphitization. The elastic modulus remained relatively constant across all temperatures. Tribological tests indicated a low coefficient of friction (CoF) of 0.15 at room temperature, increasing to 0.35 at 100 °C. The CoF further rose to 0.5 at 200 °C, coinciding with increased graphitization. However, the CoF reduced to 0.45 and 0.35 at 400 °C and 500 °C, respectively, likely due to the formation of a WO3 tribo-film and the protective effect of the chromium interlayer.
Analysis of Mechanical Properties of Fiber Reinforced Concrete Using RCC and PCC
Authors: Fan Jiawei , Long Yiyu , Xu Juntao , Qiu Shumao , Qiao Wei
Published: August 2024

The addition of macro fibers to concrete pavements has been used to improve the cracking of concrete pavement, reduce slab thickness and contribute to increasing the joint spacing. A laboratory test was carried out in the study to analyze the impact of fiber reinforced concrete (FRC) on plain cement concrete (PCC) and roller compacted concrete (RCC), determining the flexural strength by performing ASTM-1609 tests and compressive strength by ASTM C-39 tests. Two synthetic fiber types selected with different geometries and different dosages (0.25% and 0.5% by volume) were tested for both RCC and PCC. To examine the effect of fiber contents and property, statistical testing was done using strength-test data. The test result showed that flexural strength was not affected by fibers. As fiber content increased, both residual strength (F600 and F150) and specimen toughness (T150) increased for each fiber type. To the contrary, the compressive strength of specimens with higher fiber contents was lower in every case. Fiber properties including length and shape affected the residual strength of RCC more, than PCC. It is notable that the residual strength of RCC and PCC with the same fiber condition is very similar, even though the mix design and compressive and flexural strengths are different. In this paper, the strength-test data results are discussed, and the factors affecting the test results and the limitations of the testing methods are suggested.
Fabrication of Thermally Evaporated CuIx Thin Films and Their Characteristics for Solar Cell Applications
Authors: Jeon Kiseok , Park Min-Joon , Youn Sung-Min , Lim Sangwoo , Jeong Chaehwan
Published: August 2024

Carrier-selective contacts (CSCs) for high-efficiency heterojunction solar cells have been widely studied due to their advantages of processing at relatively low temperatures and simple fabrication processes. Transition metal oxide (TMO) (e.g., molybdenum oxide, vanadium oxide, and tungsten oxide) thin films are widely used as hole-selective contacts (HSCs, required work function for Si solar cells > 5.0 eV). However, when TMO thin films are used, difficulties are faced in uniform deposition. In this study, we fabricated a copper (I) iodide (CuI) thin film (work function > 5.0 eV) that remained relatively stable during atmospheric exposure compared with TMO thin films and employed it as an HSC layer in an n-type Si solar cell. To facilitate efficient hole collection, we conducted iodine annealing at temperatures of 100–180 °C to enhance the film’s electrical characteristics (carrier density and carrier mobility). Subsequently, we fabricated CSC Si solar cells using the annealed CuIx layer, which achieved an efficiency of 6.42%.
Contribution of Dung Beetles to the Enrichment of Soil with Organic Matter and Nutrients under Controlled Conditions
Authors: Hajji Hasnae , Janati-Idrissi Abdellatif , Taybi Abdelkhaleq Fouzi , Lumaret Jean-Pierre , Mabrouki Youness
Published: August 2024

Dung beetles are important ecosystem engineers as they bury manure produced by animals and contribute to nutrient cycling. This study assessed the impact of four dung beetle species, a roller (Gymnopleurus sturmi) and three tunnelers (Onthophagus vacca, Onthophagus marginalis subsp. andalusicus and Euonthophagus crocatus), on manure removal and soil fertility by using microcosms in a greenhouse setting. The four species contributed significantly to the removal of manure from the soil surface and increased the nutrient content of the soil, notably potassium, phosphorus, and nitrogen, but the amount varied depending on the species. These results highlight the importance of dung beetles in facilitating soil organic matter and nutrient flows and the need to preserve their populations to support the sustainability of grazing systems.
The Northern Red Sea (Shushah Island) Coral Health Inferred from Benthic Foraminifers
Authors: Öğretmen Nazik , Angulo-Preckler Carlos , Aranda Manuel , Duarte Carlos M. , Westphal Hildegard
Published: August 2024

The northeastern Red Sea (Saudi Arabia) is currently being transformed to become a global hub of economic activity and tourism. This transformation requires the development of pristine coastal areas into populated and dynamic settlements. At the same time, the northern Red Sea is considered a climate refuge for corals in changing climate conditions, and efforts to preserve and protect marine biodiversity are being proposed. Accordingly, foraminifers are an efficient tool to assess and monitor their associated coral reefs’ health. This study reports a modern-day health assessment of the corals of Shushah Island (Saudi Arabia) in the northeastern Red Sea as a reference for future monitoring as inferred by applying the FoRAM Index method. In general, our results revealed healthy conditions conducive to coral growth, yet some precautions and regular assessments are recommended.
Impact of Different Sources of Anthropogenic Pollution on the Structure and Distribution of Antarctic Marine Meiofauna Communities
Authors: França Débora A.A. , Ingels Jeroen , Stark Jonathan S. , da Silva Renan B. , de França Flávia J.L. , dos Santos Giovanni A.P.
Published: August 2024

Human influence on Antarctic marine ecosystems is a growing concern, despite limited information being available. This study investigated the coastal meiofauna and environmental parameters of 10 locations, 4 of which served as reference points (OB1, OB2, OB3, and McGrady) and 6 which were impacted by different levels of human activity in the past and present (Wharf, Wilkes, BBIN, BBMID, BBOUT, and Shannon) in East Antarctica. Environmental variables such as metals, total petroleum hydrocarbons (TPHs), polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), grain size, organic matter content, and nutrients were measured for analysis. Locations close to human activities showed higher concentrations of pollutants (metals, TPHs, PBDEs, PCBs) and greater variations in meiofauna diversity compared to the reference sites (OB1, OB2, OB3, and McGrady). In the area impacted by the Casey Station waste dump (Brown Bay), the meiofauna community at the location furthest from the pollution (BBOUT) source exhibited greater diversity compared to the closest location (BBIN). In addition to metals and TPHs, particle size was also correlated with community patterns, with finer sediments associated with more impacted sites, facilitating the accumulation of toxic compounds. These results contribute to the understanding of the role and impact of pollutants on meiofauna biodiversity in Antarctic coastal ecosystems.
Sinus Irrigation with N-Acetylcysteine after Endoscopic Sinus Surgery for Chronic Rhinosinusitis: A Preliminary Report of a Single-Blind Randomized Controlled Trial
Authors: Wee Jee Hye , Park Joo Hyun , Park Min Woo , Choi Young Seok , Jung Hahn Jin
Published: August 2024

Nasal irrigation is crucial following endoscopic sinus surgery (ESS), especially for managing chronic rhinosinusitis (CRS). This study assessed the effectiveness of N-acetylcysteine (NAC) irrigation during the post-ESS period of patients with CRS without nasal polyposis. In this prospective, single-blind randomized controlled trial, 49 patients (NAC, n = 24; saline, n = 25) undergoing ESS were assigned to receive either NAC or saline irrigations twice daily for a month. The preoperative and postoperative assessments conducted included Lund–Macka (LM) and Lund–Kennedy (LK) endoscopic scores, the Nasal Obstruction Symptom Evaluation (NOSE) scale, and the Sino-Nasal Outcome Test-20 (SNOT-20). At 2 weeks, 1 month, and 3 months after the operation, endoscopic findings and symptoms were evaluated. Both groups showed no differences in age, sex, LM and LK scores, NOSE scale, and SNOT-20 preoperatively. In terms of the endoscopic findings regarding the sinonasal mucosa after ESS, the NAC group had slightly lower scores 2 weeks, 1 month, and 3 months after the operation, but this difference was not statistically significant. The NAC group showed significant improvement in VAS scores, namely, postnasal drip (1.0, p = 0.041), smell dysfunction (0.8, p = 0.003), and crust (1.5, p = 0.034), compared to the control group’s scores of 2.6, 4.7, and 3.6, respectively, 2 weeks after the operation, although no significant differences were observed in VAS scores for any symptoms 1 and 3 months after the operation. NAC was well tolerated, and no adverse events were reported. NAC irrigation showed benefits over saline irrigation in terms of improving postnasal drip, smell dysfunction, and crust after ESS for CRS without nasal polyposis in the immediate postoperative period.