bluuebunny/crossref_metadata_2025 huggingface dataset:
Loneliness and Healthcare Use in Older Adults: Evidence From a Nationally Representative Cohort in Northern Ireland—A Cross-Sectional Replication Study
Authors: Burns Annette , Leavey Gerard , O'Sullivan Roger
Published: May 2021

Background: Few have explored associations between loneliness and healthcare use independent of health and health behaviors. Recent indication of gender effects also requires validation across health service and cultural settings. We investigated the associations among loneliness, health and healthcare use (HCU) in older adults including stratification to investigate whether associations differed by gender.

Methods: Secondary analysis of a nationally representative sample of 8,309 community-dwelling adults aged 50 and over from the Northern Ireland Cohort for the Longitudinal Study of Aging. Primary outcomes were: self-reported General Practice (GP) and emergency department (ED) visits in past year. Negative binomial and logistic regression analysis were used to investigate associations between loneliness and HCU, later adjusting for potential confounders (health and health behaviors).

Results: Loneliness was consistently positively associated with both GP and ED visits (with IRRs ranging from 1.10 to 1.49 for GP visits, 1.16 to 1.98 for ED visits and ORs ranging from 1.13 to 1.51 for reporting at least one ED visit). With addition of health and health behaviors, all associations between loneliness and HCU became non-significant, excepting a small independent association between UCLA score and GP visits [IRR 1.03 (95% CI 1.01–1.05)]. Stratification of models revealed no gender effects.

Conclusion: All but one association between loneliness and HCU became non-significant when health and health behaviors were included. The remaining association was small but implications remain for health service resources at population level. No gender effects were present in contrast to recent findings in the Republic of Ireland. Further studies on gender, loneliness and healthcare use needed.
The Enzymatic Digestion of Pomaces From Some Fruits for Value-Added Feed Applications in Animal Production
Authors: Kithama Munene , Hassan Yousef I. , Guo Keyi , Kiarie Elijah , Diarra Moussa S.
Published: May 2021

With the noticed steady increase of global demand for animal proteins coupled with the current farming practices falling short in fulfilling the requested quantities, more attention is being paid for means and methods intended to maximize every available agricultural-resource in a highly sustainable fashion to address the above growing gap between production and consumers' demand. Within this regard, considerable efforts are being invested either in identifying new animal feed ingredients or maximizing the utilization of already established ones. The public preference and awareness of the importance of using waste products generated by fruit-dependent industries (juice, jams, spirits, etc.) has improved substantially in recent years where a genuine interest of using the above waste(s) in meaningful applications is solidifying and optimization-efforts are being pursued diligently. While many of the earlier reported usages of fruit pomaces as feedstuffs suggested the possibility of using minimally processed raw materials alone, the availability of exogenous digestive and bio-conversion enzymes is promising to take such applications to new un-matched levels. This review will discuss some efforts and practices using exogenous enzymes to enhance fruit pomaces quality as feed components as well as their nutrients' accessibility for poultry and swine production purposes. The review will also highlight efforts deployed to adopt numerous naturally derived and environmentally friendly catalytic agents for sustainable future feed applications and animal farming-practices.
Evaluation of Chronic Kidney Disease Progression in Dogs With Therapeutic Management of Risk Factors
Authors: Perini-Perera Sofía , Del-Ángel-Caraza Javier , Pérez-Sánchez Alicia Pamela , Quijano-Hernández Israel Alejandro , Recillas-Morales Sergio
Published: May 2021

This research was performed to describe the characteristics of the progression of naturally occurring chronic kidney disease (CKD) in dogs, together with the management of identified risk factors, following the International Renal Interest Society recommendations. Dogs diagnosed and staged with CKD, and with a longitudinal follow-up from the moment of diagnosis of up to a maximum of 730 days, were included. A total of 545 dogs that presented risk factors for the development of CKD were analyzed, out of which 36 met the inclusion criteria. Advanced age was identified in 80.6% of cases. Initiation risk factors were represented by inflammatory/infectious diseases, history of anesthetic-surgical procedures, heart disease, neoplasms, endocrinopathies, and exposure to nephrotoxic drugs. During the follow-up period, progression of CKD was identified in 47.2% of the cases, being more salient in advanced stages. Serum symmetric dimethyl arginine (SDMA) was the only glomerular filtration rate (GFR) marker which displayed differences among studied times during early stages of CKD, associated with the disease progression and decline of renal function. A significant difference between the survival curves in early and advanced CKD stages was observed. The factors related to decreased survival were hyperphosphatemia, anemia, and low body condition score (BCS). No differences were found between the presence of arterial hypertension and renal proteinuria and decreased survival. Furthermore, CKD diagnosis based on the persistent finding of abnormalities in early disease markers, such as serum symmetric dimethyl arginine increase and/or renal proteinuria, and timely therapeutic management of risk factors, allowed for CKD stabilization, reducing progression to advanced stages, and favoring higher survival rates.
The Effects of 7 Days of Feeding Pulse-Based Diets on Digestibility, Glycemic Response and Taurine Levels in Domestic Dogs
Authors: Quilliam Chloe , Ren Yikai , Morris Tressa , Ai Yongfeng , Weber Lynn P.
Published: May 2021

Grain-based carbohydrate sources such as rice comprise 30–50% of commercial pet foods. Some pet foods however have removed the use of grains and have instead incorporated pulses, such as peas and lentils, resulting in grain-free diets. The hypothesis was dog diets with higher levels of dietary fiber will produce a low glycemic response due to decreased rates of digestion and lowered bioavailability of all macronutrients and increased fecal bile salt excretion. This in turn was hypothesized to produce lower plasma concentrations of cysteine, methionine and taurine after 7 days of feeding each test diet in dogs. Six diets were formulated at an inclusion level of 20% available carbohydrate, using white rice flour (grain) or whole pulse flours from smooth pea, fava bean, red lentil or 2 different wrinkled pea varieties (CDC 4,140–4 or Amigold) and fed to beagles in a randomized, cross-over, blinded design. After 7 days feeding each diet, fasting blood glucose was the lowest in the lentil (3.5 ± 0.1 mmol/L) and wrinkled pea (4,140–4; 3.6 ± 0.1 mmol/L) diet periods, while peak glucose levels was lowest after feeding the lentil diet (4.4 ± 0.1 mmol/L) compared to the rice diet. Total tract apparent digestibility of all macronutrients as well as taurine differed among diets yet plasma taurine was not outside normal range. Decreased macronutrient and amino acid digestibility was associated with increasing amylose and dietary fiber content but the specific causative agent could not be determined from this study. Surprisingly, digestibility decreases were not due to increased bile salt loss in the feces since increasing dietary fiber content led to decreased fecal bile salt levels. In conclusion, although pulse-based canine diets have beneficial low glycemic properties, after only 7 days, these pulse-based diets decrease macronutrient and amino acid digestibility. This is likely related at least in part to the lower animal protein content, but on a long-term basis could put domestic dogs at risk for low taurine and dilated cardiomyopathy.
Assessment of Agricultural Drought Using Soil Water Deficit Index Based on ERA5-Land Soil Moisture Data in Four Southern Provinces of China
Authors: Zhang Ruqing , Li Lu , Zhang Ye , Huang Feini , Li Jianduo , Liu Wei , Mao Taoning , Xiong Zili , Shangguan Wei
Published: May 2021

It is important to accurately assess agricultural drought because of its harmful impacts on the ecosystem and economy. Soil moisture reanalysis datasets provide an important way to assess agricultural drought. In this study, the ERA5-Land surface and subsurface soil moisture was used to estimate the soil water deficit index (SWDI) in four southern provinces of China. The ERA5-Land dataset was evaluated with in situ soil moisture observations from agrometeorological stations. Agricultural drought was assessed for three climate zones at a weekly scale from 2017 to 2019 and was compared with the atmospheric water deficit (AWD). It was found that both ERA5-Land soil moisture and the derived SWDI have relatively high accuracy, and the wet bias in the ERA5-Land dataset can be reduced by the calculation of the SWDI. The subsurface layer has better performance than the surface layer in drought monitoring, though they are highly correlated. Different climate zones demonstrate different drought periods and drought severity, and the temperate climate zone with no dry season has less droughts. The most severe droughts with the largest spatial extent occurred in the early winter, especially in 2019. Differences in the SWDI and AWD are mainly shown in southwestern Yunnan. The results of this study have important reference values for drought risk management.
Dicamba Injury on Soybean Assessed Visually and with Spectral Vegetation Index
Authors: Marques Matheus Gregorio , da Cunha João Paulo Arantes Rodrigues , Lemes Ernane Miranda
Published: May 2021

The recent availability of soybean cultivars with resistance to dicamba herbicide has increased the risk of injury in susceptible cultivars, mainly as a result of particle drift. To predict and identify the damage caused by this herbicide requires great accuracy. The objective of this work was to evaluate the injury caused by the simulated drift of dicamba on soybean (nonresistant to dicamba) plants assessed visually and using the Triangular Greenness Index (TGI) from images obtained from Remotely Piloted Aircraft (RPA). The study was conducted in a randomized complete block design with four replications during the 2019/2020 growing season, and the treatments consisted of the application of six doses of dicamba (0, 0.28, 0.56, 5.6, 28, and 112 g acid equivalent dicamba ha−1) on soybean plants at the third node growth stage. For the evaluation of treatments using the TGI technique, spectral data acquired through a Red Green Blue (RGB) sensor attached to an RPA was used. The variables studied were the visual estimation of injury, TGI response at 7 and 21 days after application, plant height, and crop yield. The exposure to the herbicide caused a reduction in plant height and crop yield. Vegetation indices, such as TGI, have the potential to be used in the evaluation of injury caused by dicamba, and may be used to cover large areas in a less subjective way than visual assessments.
Genomic Imprinting at the Porcine DIRAS3 Locus
Authors: Ahn Jinsoo , Hwang In-Sul , Park Mi-Ryung , Hwang Seongsoo , Lee Kichoon
Published: May 2021

The epigenetic mechanisms underlying genomic imprinting include DNA methylation and monoallelic expression of genes in close proximity. Although genes imprinted in humans and mice have been widely characterized, there is a lack of detailed and comprehensive studies in livestock species including pigs. The purpose of this study was to investigate a detailed methylation status and parent-of-origin-specific gene expression within the genomic region containing an underexamined porcine DIRAS3 locus. Through whole-genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-seq) of porcine parthenogenetic embryos and analyses of public RNA-seq data from adult pigs, DNA methylation and monoallelic expression pattern were investigated. As a result, maternal hypermethylation at the DIRAS3 locus and hypothalamus-specific and monoallelic expression of the DIRAS3 gene were found in pigs. In conclusion, the findings from this study suggest that the presence of maternal hypermethylation, or imprints, might be maintained and related to monoallelic expression of DIRAS3 during pig development.
Effects of Medicinal Plants and Organic Selenium against Ovine Haemonchosis
Authors: Komáromyová Michaela , Mravčáková Dominika , Petrič Daniel , Kucková Katarína , Babják Michal , Dolinská Michaela Urda , Königová Alžbeta , Maďarová Michaela , Pruszyńska-Oszmałek Ewa , Cieslak Adam , Čobanová Klaudia , Váradyová Zora , Várady Marián
Published: May 2021

The objective of this study was to determine the effect of traditional medicinal plants typical to Central Europe as well as organic selenium on increasing the resistance of lambs to gastrointestinal nematode (GIN) infection with Haemonchus contortus. 21 female lambs were infected with third-stage larvae of H. contortus on the day (D) 0 and re-infected on D49 and D77. The animals were divided into three groups based on a treatment diet: a basal diet (control), a diet enriched with dry plants (Herbmix), and a diet enriched with selenized yeast (Selplex). The number of eggs per gram (EPG) of feces was quantified on D21, D28, D35, D42, D49, D56, D63, D70, D77, D84, D91, D98, D105, D112, and D119. The mean reductions in EPG on D28 were 43.4 and 28.6% for Selplex and Herbmix, respectively. The reduction in egg output was nearly uniform throughout the experiment for Selplex. However, for Herbmix the mean reduction was only 19.8% up to D91 and 46.1% after D91. Glutathione peroxidase activity in the blood from D35 to D98 was two to three-fold higher for Selplex than the other groups. Both supplements slowed the dynamics of GIN infection and gradually increased the resistance of lambs against ovine haemonchosis.
Toward Precision Feeding Regarding Minerals: What Is the Current Practice in Commercial Dairy Herds in Québec, Canada?
Authors: Duplessis Mélissa , Fadul-Pacheco Liliana , Santschi Débora E. , Pellerin Doris
Published: May 2021

This analysis is performed to obtain information on the current situation regarding phosphorus (P), cobalt (Co), copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) concentrations in cow diets of commercial dairy herds in Québec, Canada, and to compare them with National Research Council recommendations. Data are collected on 100 Holstein dairy herds in Québec, Canada, and 4430 cows were involved. Rations are analyzed for selected minerals and cow requirements relative to the recommendations were calculated. Median percentages of mineral recommendations fulfilled by forage were 55%, 196%, 54%, 776%, 181%, and 44% for P, Co, Cu, Fe, Mn, and Zn, respectively. Daily dietary concentrations of P, Cu, Mn, and Zn decreased as lactation progressed, whereas Co and Fe were stable throughout lactation. Phosphorus was the mineral fed the closest to the requirements, cows below 21 days in milk were even underfed by 11%. All studied trace minerals were fed in excess for the majority of cows. Cobalt was fed on average 480% above requirements regardless of the stage of lactation. For Cu, Fe, Mn, and Zn, rations for cows below 21 days in milk were fed 23% (95% confidence interval: 15–32), 930% (849–1019), 281% (251–314), and 35% (22–47) above the recommendations, respectively, and were closer to the requirements than after 21 days in milk. These results show that most nutritionists are aware that precision feeding regarding P is important to minimize detrimental environmental impacts of dairy production. However, some efforts should be made to limit trace mineral overfeeding to ensure environmental resiliency.
Liveweight and Sex Effects on Instrumental Meat Quality of Rubia de El Molar Autochthonous Ovine Breed
Authors: Miguel Eugenio , Blázquez Belén , Ruiz de Huidobro Felipe
Published: May 2021

The effects of sex and weight on instrumental meat quality characteristics of Rubia de El Molar autochthonous ovine breed were studied. Four weight groups (10, 15, 20 and 25 kg, each of the groups with seven males and seven females) were assessed. A decrease of longissimus thoracis muscle (LT) lightness from 43.39 for 10 kg lambs to 38.84 for 25 kg group was observed. LT redness and yellowness indices and chromaticity values increased as weight increased. The percentage of juice expelled increased from 11.52 (10 kg) to 17.15 (15 kg). The percentage of intramuscular fat in biceps femoris (BF) and supraespinatus (SE) muscles increased as weight increased. Warner–Bratzler shear force (WBSF) in raw meat and hardness in cooked meat increased as liveweight increased. However, a decrease in the amount of soluble collagen in quadriceps femoris (QF), biceps femoris (BF) and infraespinatus (IE) muscles was observed as weight increased. Sex effect was only observed for intramuscular fat content in QF and BF muscles (2.4% in males and 2.9 in females) and WBSF in raw meat (26.12 N for males and 21.25 N for females). Weight had a greater effect than sex on Rubia de El Molar meat quality characteristics.
Characterization of Pollutant Emissions from Typical Material Handling Equipment Using a Portable Emission Measurement System
Authors: Pang Kaili , Meng Xiangrui , Ma Shuai , Yin Ziyuan
Published: May 2021

Non-road equipment has been an important source of pollutants that negatively affect air quality in China. An accurate emission inventory for non-road equipment is therefore required to improve air quality. The objective of this paper was to characterize emissions from typical diesel-fueled material handling equipment (loaders and cranes) using a portable emission measurement system. Instantaneous, modal, and composite emissions were quantified in this study. Three duty modes (idling, moving, and working) were used. Composite emission factors were estimated using modal emissions and time-fractions for typical duty cycles. Results showed that emissions from loaders and cranes were higher and more variable for the moving and working modes than the idling mode. The estimated fuel-based CO, HC, NO, and PM2.5 composite emission factors were 21.7, 2.7, 38.2, and 3.6 g/(kg-fuel), respectively, for loaders, and 8.7, 2.4, 28.3, and 0.3 g/(kg-fuel), respectively, for cranes. NO emissions were highest and should be the main focus for emission controls. CO, HC, NO, and PM2.5 emissions measured were different from emission factors in the US Environmental Protection Agency NONROAD model and the Chinese National Guideline for Emission Inventory Development for Non-Road Equipment. This indicates that improving emission inventory accuracy for non-road equipment requires more real-world emission measurements.
Temperature Response to Changes in Vegetation Fraction Cover in a Regional Climate Model
Authors: Jiménez-Gutiérrez Jose Manuel , Valero Francisco , Ruiz-Martínez Jesús , Montávez Juan Pedro
Published: May 2021

Vegetation plays a key role in partitioning energy at the surface. Meteorological and Climate Models, both global and regional, implement vegetation using two parameters, the vegetation fraction and the leaf area index, obtained from satellite data. In most cases, models use average values for a given period. However, the vegetation is subject to strong inter-annual variability. In this work, the sensitivity of the near surface air temperature to changes in the vegetation is analyzed using a regional climate model (RCM) over the Iberian Peninsula. The experiments have been designed in a way that facilitates the physical interpretation of the results. Results show that the temperature sensitivity to vegetation depends on the time of year and the time of day. Minimum temperatures are always lower when vegetation is increased; this is due to the lower availability of heat in the ground due to the reduction of thermal conductivity. Regarding maximum temperatures, the role of increasing vegetation depends on the available moisture in the soil. In the case of hydric stress, the maximum temperatures increase, and otherwise decrease. In general, increasing vegetation will lead to a higher daily temperature range, since the decrease in minimum temperature is always greater than the decrease for maximum temperature. These results show the importance of having a good estimate of the vegetation parameters as well as the implications that vegetation changes due to natural or anthropogenic causes might have in regional climate for present and climate change projections.
Numerical Study on the Plume Behavior of Multiple Stacks of Container Ships
Authors: Xu Yine , Yu Qi , Zhang Yan , Ma Weichun
Published: May 2021

This paper showed different plume behaviors of exhausts from different number of stacks of the container ship, using CFD code PHOENICS version 6.0. The plume behavior was quantitatively analyzed by mass fraction of the pollutant in the exhaust and plume heights. Three simplified typical configurations were constructed by CFD according to the investigation of container ships. The configurations included a single main stack (BL1), one main stack and multiple auxiliary stacks (BL2), and two main stacks and multiple auxiliary stacks (BL3). All the main stacks had the same emission characteristics, and all the auxiliary stacks had the same emission characteristics. The results show that the transmission and diffusion characteristics of the exhaust from multiple stacks are different from those of the exhaust from a single stack. In BL2 and BL3 simulations, the maximum mass fraction of SO2 in the exhaust (C1max) of multiple stack emissions was approximately 329% and 269% higher than that of single stack emissions over the main stack, respectively, and the plume height of multiple stack emissions is higher than that of single stack emissions. In BL2 and BL3 simulations, the plume height of multiple stack emissions was 41% and 75% higher than that of single stack emissions, respectively. The increase of C1max, due to multiple stack emissions, is weakened as the distance of the stacks increase. The difference in plume behavior between multiple stack emissions and single stack emissions is of great significance for air quality management and pollution control in port areas.
Single-Stage Deep Brain Stimulator Placement for Movement Disorders: A Case Series
Authors: Brooks Arrin , Hoyt Alastair T.
Published: May 2021

With more than two decades of experience and thousands of patients treated worldwide, deep brain stimulation (DBS) has established itself as an efficacious and common surgical treatment for movement disorders. However, a substantial majority of patients in the United States still undergo multiple, “staged” surgeries to implant a DBS system. Despite several reports suggesting no significant difference in complications or efficacy between staged and non-staged approaches, the continued use of staging implies surgeons harbor continued reservations about placing all portions of a system during the index procedure. In an effort to eliminate multiple surgeries and simplify patient care, DBS implantations at our institution have been routinely performed in a single surgery over the past four years. Patients who underwent placement of new DBS systems at our institution from January 2016 to June 2019 were identified and their records were reviewed. Revision surgeries were excluded. Total operative time, length of stay and rates of surgical site infections, lead fracture or migration, and other complications were evaluated. This series expands the body of evidence suggesting placement of a complete DBS system during a single procedure appears to be an efficacious and well-tolerated option.
Microsurgical Anatomy of the Inferomedial Paraclival Triangle: Contents, Topographical Relationships and Anatomical Variations
Authors: Wysiadecki Grzegorz , Radek Maciej , Tubbs R. Shane , Iwanaga Joe , Walocha Jerzy , Brzeziński Piotr , Polguj Michał
Published: May 2021

The inferomedial triangle is one of the two surgical triangles in the paraclival subregion of the skull base. It is delineated by the posterior clinoid process, the dural entrance of the trochlear nerve and the dural entrance of the abducens nerve. The aim of the present article is to describe the anatomical variations within the inferomedial triangle. Measurements of the triangle’s borders and area were supplemented by detailed observations of the topographical anatomy and various arrangements of its contents. Nine adult cadaveric heads (18 sides) and 28 sagittal head sections were studied. The mean area of the inferomedial triangle was estimated to be 60.7 mm2. The mean lengths of its medial, lateral and superior borders were 16.1 mm, 11.9 mm and 10.4 mm, respectively. The dorsal meningeal artery was identified within the inferomedial triangle in 37 out of 46 sides (80.4%). A well-developed petrosphenoidal ligament of Grüber was identified within the triangle on 36 sides (78.3%). Although some structures were variable, the constant contents of the inferomedial triangle were the posterior petroclinoid dural fold, the upper end of the petroclival suture, the gulfar segment of the abducens nerve and the posterior genu of the intracavernous internal carotid artery.
Mental Health Screening Needs and Preference in Treatment Types and Providers in African American and Asian American Older Adults
Authors: Lee Minsun , Lu Wenyue , Mann-Barnes Tyrell , Nam Jin-Hyeok , Nelson Julie , Ma Grace X.
Published: May 2021

Older African Americans and Asian Americans in the U.S. underuse mental health services, despite their vulnerability to diverse mental health problems. This study examined their perspectives on the importance of various mental health problems, mental health treatment, and provider type preference. A total of 243 participants residing in Philadelphia were recruited through community-based organizations. Chi-square, ANOVA, and logistic regression were conducted to examine ethnic differences in demographic characteristics, mental health screening needs, and treatment preferences. African Americans were more likely to endorse the screening needs for depression (AOR: 3.77; 95% CI: 1.19–11.93, p < 0.05) and less likely to endorse the screening needs for suicide (AOR: 0.24; 95% CI: 0.08–0.76, p < 0.05) compared to Asian Americans. For treatment preferences, African Americans were more likely to seek help from primary care physicians (AOR: 8.26; 95% CI: 1.71–32.86, p < 0.01) and less likely to prefer medication as a treatment option (AOR: 0.36; 95% CI: 0.09–0.79, p < 0.05) than Asian Americans. African Americans and Asian Americans prioritized mental health screening needs differently and had different treatment preferences, indicating that matching community needs and preferences regarding mental health services is critical to improve mental service utilization rates in the targeted populations.
Targeting BTK Signaling in the Microenvironment of Solid Tumors as a Feasible Cancer Therapy Option
Authors: Messex Justin K. , Liou Geou-Yarh
Published: May 2021

The cell environment plays a pivotal role in determining cellular outcome, as well as cancer initiation, progression, and dissemination. Within this environment, in addition to the structural components, such as the extracellular matrix, there are various types of cells surrounding the tumor cells. Communication among these cells and the tumor cells via signaling pathways is important for tumor growth. Originally discovered in patients with immunodeficiency X-linked gammaglobulinemia, the Bruton’s tyrosine kinase (BTK) signaling pathway, known for its role in B cell maturation, is critical to cancer cell proliferation, metastasis and evasion of cancer eliminating cells. Given that BTK inhibitors have been FDA approved for chronic lymphocytic leukemia/small lymphocytic lymphoma and that the majority of BTK studies have been focused on B cells, the use of BTK inhibitors as a future treatment strategy of solid tumors has yet to be evaluated. In this review, we summarize studies analyzing BTK signaling within the cells found in the tumor microenvironment, as well as clinical trial where BTK inhibitors are currently being used to target the tumor microenvironment as a way to combat solid tumors.
Novel Human Antibodies to Insulin Growth Factor 2 Receptor (IGF2R) for Radioimmunoimaging and Therapy of Canine and Human Osteosarcoma
Authors: Broqueza Jaline , Prabaharan Chandra B. , Andrahennadi Samitha , Allen Kevin J. H. , Dickinson Ryan , MacDonald-Dickinson Valerie , Dadachova Ekaterina , Uppalapati Maruti
Published: May 2021

Etiological and genetic drivers of osteosarcoma (OS) are not well studied and vary from one tumor to another; making it challenging to pursue conventional targeted therapy. Recent studies have shown that cation independent mannose-6-phosphate/insulin-like growth factor-2 receptor (IGF2R) is consistently overexpressed in almost all of standard and patient-derived OS cell lines, making it an ideal therapeutic target for development of antibody-based drugs. Monoclonal antibodies, targeting IGF2R, can be conjugated with alpha- or beta-emitter radionuclides to deliver cytocidal doses of radiation to target IGF2R expression in OS. This approach known as radioimmunotherapy (RIT) can therefore be developed as a novel treatment for OS. In addition, OS is one of the common cancers in companion dogs and very closely resembles human OS in clinical presentation and molecular aberrations. In this study, we have developed human antibodies that cross-react with similar affinities to IGF2R proteins of human, canine and murine origin. We used naïve and synthetic antibody Fab-format phage display libraries to develop antibodies to a conserved region on IGF2R. The generated antibodies were radiolabeled and characterized in vitro and in vivo using human and canine OS patient-derived tumors in SCID mouse models. We demonstrate specific binding to IGF2R and tumor uptake in these models, as well as binding to tumor tissue of canine OS patients, making these antibodies suitable for further development of RIT for OS
Magnetic Nanoparticles—A Multifunctional Potential Agent for Diagnosis and Therapy
Authors: Chouhan Raghuraj Singh , Horvat Milena , Ahmed Jahangeer , Alhokbany Norah , Alshehri Saad M. , Gandhi Sonu
Published: May 2021

Magnetic nanoparticles gained considerable attention in last few years due to their remarkable properties. Superparamaganetism, non-toxicity, biocompatibility, chemical inertness, and environmental friendliness are some of the properties that make iron oxide nanoparticles (IONPs) an ideal choice for biomedical applications. Along with being easily tuneable and a tailored surface for conjugation of IONPs, their physio-chemical and biological properties can also be varied by modifying the basic parameters for synthesis that enhances the additional possibilities for designing novel magnetic nanomaterial for theranostic applications. This review highlights the synthesis, surface modification, and different applications of IONPs for diagnosis, imaging, and therapy. Furthermore, it also represents the recent report on the application of IONPs as enzyme mimetic compounds and a contrasting agent, and its significance in the field as an anticancer and antimicrobial agent.
The Role of HSP90 in Preserving the Integrity of Genomes Against Transposons Is Evolutionarily Conserved
Authors: Specchia Valeria , Bozzetti Maria Pia
Published: May 2021

The HSP90 protein is a molecular chaperone intensively studied for its role in numerous cellular processes both under physiological and stress conditions. This protein acts on a wide range of substrates with a well-established role in cancer and neurological disorders. In this review, we focused on the involvement of HSP90 in the silencing of transposable elements and in the genomic integrity maintenance. The common feature of transposable elements is the potential jumping in new genomic positions, causing chromosome structure rearrangements, gene mutations, and influencing gene expression levels. The role of HSP90 in the control of these elements is evolutionarily conserved and opens new perspectives in the HSP90-related mechanisms underlying human disorders. Here, we discuss the hypothesis that its role in the piRNA pathway regulating transposons may be implicated in the onset of neurological diseases.
Chromothripsis—Explosion in Genetic Science
Authors: Shorokhova Mariia , Nikolsky Nikolay , Grinchuk Tatiana
Published: May 2021

Chromothripsis has been defined as complex patterns of alternating genes copy number changes (normal, gain or loss) along the length of a chromosome or chromosome segment (International System for Human Cytogenomic Nomenclature 2020). The phenomenon of chromothripsis was discovered in 2011 and changed the concept of genome variability, mechanisms of oncogenic transformation, and hereditary diseases. This review describes the phenomenon of chromothripsis, its prevalence in genomes, the mechanisms underlying this phenomenon, and methods of its detection. Due to the fact that most often the phenomenon of chromothripsis occurs in cancer cells, in this review, we will separately discuss the issue of the contribution of chromothripsis to the process of oncogenesis.
Beiging Modulates Inflammatory Adipogenesis in Salt-Treated and MEK6–Transfected Adipocytes
Authors: Kang Songjoo , Lee Myoungsook
Published: May 2021

To investigate whether the beiging process changes the interactive effects of salt and MEK6 gene on inflammatory adipogenesis, the salt treatment (NaCl 50 mM) and MEK6 transfection of Tg(+/+) cells were performed with white adipocytes (WAT) and beige-like-adipocytes (BLA). BLA induced by T3 were confirmed by UCP-1 expression and the MEK6 protein was 3.5 times higher in MEK6 transfected WAT than the control. The adipogenic genes, PPAR-γ and C/EBP-α, were 1.5 times more highly expressed in the salt-treated groups than the non-salt-treated groups, and adipogenesis was greatly increased in Tg(+/+) WAT compared to non-transfected Tg(−/−). The adipogenesis induced by salt treatment and MEK6 transfection was significantly reduced in BLA. The inflammatory adipocytokines, TNF-α, IL-1β, and IL-6, were increased in the salt-treated Tg(+/+) WAT, but an anti-inflammation biomarker, the adiponectin/leptin ratio, was reduced in Tg(+/+), to tenth of that in Tg(−/−). However, the production of adipocytokines in WAT was strongly weakened in BLA, although a combination of salt and MEK6 transfection had the most significant effects on inflammation in both WAT and BLA. Oxygen consumption in mitochondria was maximized in salt-treated and MEK6 transfected WAT, but it was decreased by 50% in BLA. In conclusion, beiging controls the synergistic effects of salt and MEK6 on adipogenesis, inflammation, and energy expenditure.
New Treatment Strategy Targeting Galectin-1 against Thyroid Cancer
Authors: Gheysen Laetitia , Soumoy Laura , Trelcat Anne , Verset Laurine , Journe Fabrice , Saussez Sven
Published: May 2021

Although the overall survival rate of papillary or follicular thyroid cancers is good, anaplastic carcinomas and radio iodine refractory cancers remain a significant therapeutic challenge. Galectin-1 (Gal-1) is overexpressed in tumor cells and tumor-associated endothelial cells, and is broadly implicated in angiogenesis, cancer cell motility and invasion, and immune system escape. Our team has previously demonstrated a higher serum level of Gal-1 in patients with differentiated thyroid cancers versus healthy patients, and explored, by a knockdown strategy, the effect of Gal-1 silencing on cell proliferation and invasion in vitro, and on tumor and metastasis development in vivo. OTX008 is a calixarene derivative designed to bind the Gal-1 amphipathic β-sheet conformation and has previously demonstrated anti-proliferative and anti-invasive properties in several cancer cell lines including colon, breast, head and neck, and prostate cancer lines. In the current work, the impacts of OTX008 were evaluated in six thyroid cancer cell lines, and significant inhibitions of proliferation, migration, and invasion were observed in all lines expressing high Gal-1 levels. In addition, the signaling pathways affected by this drug were examined using RPPA (reverse phase protein array) and phosphoprotein expression assays, and opposite regulation of eNos, PYK2, and HSP27 by OTX008 was detected by comparing the two anaplastic lines 8505c and CAL 62. Finally, the sensitive 8505c line was xenografted in nude mice, and 3 weeks of OTX008 treatment (5 mg/kg/day) demonstrated a significant reduction in tumor and lung metastasize sizes without side effects. Overall, OXT008 showed significant anti-cancer effects both in vitro and in vivo in thyroid cancer lines expressing Gal-1, supporting further investigation of the molecular mechanisms of the drug and future clinical trials in patients with anaplastic thyroid cancer.
Influence of Surface Post-Processing on Crystal Refinement and Characteristics of Hopeite Coating by Phosphating
Authors: Liu Bing , Zheng Yongzhen , Xiao Guiyong , Chen Chuanzhong , Lu Yupeng
Published: May 2021

Hopeite (Zn3(PO4)2·4H2O) conversion coating was prepared on pure titanium using mild phosphate chemical conversion technology. In this investigation the effect of post-processing after phosphating was focused on coating microstructure, chemical composition, adherence and corrosion resistance. Different sequential order of drying and rinsing of the coated samples were carried out. The results demonstrated that the priority shift of rinsing or drying did not change the hopeite phase of the conversion coating, but played a significant role in the crystal size and coating microstructure. Compared with the traditional preparation of the coating, a more compact and uniform coating with finer crystals grains (3–5 μm) was obtained by the unconventional post-treatment of drying before rinsing, along with a higher bonding strength and corrosion resistance.
Effects of Heat Treatment on Interfacial Properties of Pinus Massoniana Wood
Authors: Wu Zhigang , Deng Xue , Li Lifen , Xi Xuedong , Tian Meifen , Yu Liping , Zhang Bengang
Published: May 2021

Understanding the interfacial changes of wood during heat treatment can facilitate the improvement of the bonding and coating processes of heat-treated wood. Steam was used as the medium to modify Pinusmassoniana wood through heat treatment at 160, 180, 200, and 220 °C. Changes to the surface characteristics after heat treatment were characterized by Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscope (SEM) and contact angle measurement. The results showed that: (1) hemicelluloses were the first to experience degradation at 160 °C, and this degradation was the most intense at 200 °C. The cellulose started experiencing obvious degradation at 200 °C, while there was less degradation of lignin at this temperature. (2) Oxygen-containing groups like hydroxyl and carbonyl were gradually reduced as temperature increased with deepened color and passivated surface. (3) Cellulose crystallinity presented a variable trend of increasing–decreasing–increasing. (4) Surface porosity and roughness of Pinus massoniana wood both increased after heat treatment. (5) The Pinus massoniana wood interface turned from hydrophilic to hydrophobic, and 180 °C was a turning point for the wettability of the Pinus massoniana wood interface.
Bulk and Surface Conformations in Solid-State Lovastatin: Spectroscopic and Molecular Dynamics Studies
Authors: Pallipurath Anuradha R. , Skelton Jonathan M. , Britton Andrew , Willneff Elizabeth A. , Schroeder Sven L. M.
Published: May 2021

Conformational flexibility in molecules can give rise to a range of functional group terminations at crystal surfaces and dynamic disorder in the bulk. In this work, we explore the conformational behavior of the drug molecule lovastatin in the crystallographically disordered solid and at crystal surfaces through a combination of computational modeling and spectroscopy. Gas-phase and periodic quantum-chemical calculations are used to study the potential energy surface associated with rotatable bonds to examine the disorder in bulk. These calculations are combined with vibrational and X-ray photoelectron spectroscopy measurements to obtain insight into the conformations in bulk and at the surface. Our MD simulations show that the bulk disorder is driven by cooperative motion of the butyl group on the S-butanoate moiety along one crystallographic direction beyond a unit cell. The calculations show that the O-H group can rotate relatively freely between two low-energy conformers in the gas phase but is locked in position by intermolecular H-bonding interactions in the bulk crystal, and we find tentative spectroscopic evidence for the second conformer being present at the surface. We also comment on the relative utility of these different techniques for studying molecular conformation in bulk and at surfaces and highlight possible areas for future developments.
Recent Developments in Flexible Transparent Electrode
Authors: Wang Tingting , Lu Kuankuan , Xu Zhuohui , Lin Zimian , Ning Honglong , Qiu Tian , Yang Zhao , Zheng Hua , Yao Rihui , Peng Junbiao
Published: May 2021

With the rapid development of flexible electronic devices (especially flexible LCD/OLED), flexible transparent electrodes (FTEs) with high light transmittance, high electrical conductivity, and excellent stretchability have attracted extensive attention from researchers and businesses. FTEs serve as an important part of display devices (touch screen and display), energy storage devices (solar cells and super capacitors), and wearable medical devices (electronic skin). In this paper, we review the recent progress in the field of FTEs, with special emphasis on metal materials, carbon-based materials, conductive polymers (CPs), and composite materials, which are good alternatives to the traditional commercial transparent electrode (i.e., indium tin oxide, ITO). With respect to production methods, this article provides a detailed discussion on the performance differences and practical applications of different materials. Furthermore, major challenges and future developments of FTEs are also discussed.
Malignant Skin Cancer Excision in Combined Therapy with Electro-Chemotherapy and Dermal Substitute
Authors: De Angelis Barbara , Balzani Alberto , Pagnotta Alessia , Tati Eleonora , Orlandi Fabrizio , D’Autilio Margarida Fernandes Lopes Morais , Cervelli Valerio , Gentile Pietro
Published: May 2021

Squamous cell carcinoma (SCC) is the second most common malignancy skin cancer. It is characterized by abnormal, accelerated growth of squamous cells (SCs). SCC occurs when DNA damage from exposure to ultraviolet radiation or other damaging agents trigger abnormal changes in the SCs, presenting as painless lesions on areas of high sun exposure, such as the dorsum of the hand and upper extremity. For most skin SCC, the surgical excision alone is standard practice. However, recent efforts in new treatment strategies have involved around adjuvant or concomitant electrochemotherapy (ECT). ECT is a non-thermal tumor ablation modality, safe and effective on any type of solid tumor. An 87-year-old patient affected by hand SCC with invasion of deep structures including tendons was treated with neoadjuvant intra-tumoral ECT sessions followed by a selective surgical removal and reconstruction of the substance loss with collagen dermal template (CDT). Two neoadjuvant intra-tumoral ECT procedures, at distance of 3 months, with the aim to reduce the tumor size before a selective surgery, were performed. This study shows that combined surgical selective excision with ECT and CDT is a valid technique for the extended-deep dorsal hand tumor lesions reconstruction.
Newcomers from the Periphery: The International Expansion of Polish Automotive Companies
Authors: Micek Grzegorz , Guzik Robert , Gwosdz Krzysztof , Domański Bolesław
Published: May 2021

The rapid growth of Central European economies has been related to inward FDI, so these countries are often regarded as dependent market economies. What is interesting in this context is the international expansion of some domestic-owned manufacturing companies from Central Europe. Looking through the lens of global production networks (GPN) and evolutionary resilience perspectives, the paper aims to identify paths and mechanisms of development of new multinational companies, namely Polish automotive firms expanding from around 2010 onwards in the global markets through acquisitions and greenfield investments in Western Europe, North America, and Asia. The authors ponder on the underlying motives and barriers of this process, the capabilities, and features of Polish-owned companies. In order to do it, both qualitative (13 interviews with representatives of new multinationals and the other stakeholders preceded by content analysis of various documents, including company annual reports in particular) and quantitative (analysis of international trade, revenues, employment, locational data) insights are used for the period covering the time span between 1999 and 2020. As the result, the international expansion of domestic-owned firms is interpreted as a specific critical conjuncture of three mechanisms: (1) development of Central-Eastern European economies since the end of communism, (2) the evolution of the Western European core, and (3) the trajectory of individual Polish firms. We argue development trajectories of the firms going global cannot be understood without taking into account the dynamic interdependence between firm-specific capabilities (including the position within GPNs) and the changing characteristics of the countries (e.g., evolving regional assets).
Energy Harvesting and Water Saving in Arid Regions via Solar PV Accommodation in Irrigation Canals
Authors: Alhejji Ayman , Kuriqi Alban , Jurasz Jakub , Abo-Elyousr Farag K.
Published: May 2021

The Egyptian irrigation system depends mainly on canals that take water from the River Nile; nevertheless, the arid climate that dominates most of the country influences the high rate of water losses, mainly through evaporation. Thus, the main objective of this study is to develop a practical approach that helps to accommodate solar photovoltaic (PV) panels over irrigation canals to reduce the water evaporation rate. Meanwhile, a solar PV panel can contribute effectively and economically to an on-grid system by generating a considerable amount of electricity. A hybrid system includes a solar PV panel and a diesel generator. Several factors such as the levelized cost of energy (LCOE), total net present cost, loss of power supply probability, and greenhouse gas emissions should be considered while developing a technoeconomically feasible grid-connected renewable integrated system. A mathematical formulation for the water loss was introduced and the evaporation loss was monthly estimated. Thus, this study also aims to enhance an innovative metaheuristic algorithm based on a cuckoo search optimizer to show the way forward for developing a technoeconomic study of an irrigation system integrated with an on-grid solar PV panel designed for a 20-year lifespan. The results are compared using the mature genetic algorithm and particle swarm optimization to delimit the optimal size and configuration of the on-grid system. The optimal technoeconomic feasibility is connected to the graphical information system to delimit the optimal length and direction of the solar PV accommodation covering the canals. Finally, based on the simulated results, the optimal sizing and configuration of the irrigation-system-integrated on-grid solar PV accommodation have less impact on the LCOE without violating any constraint and, at the same time, generating clean energy.