bluuebunny/crossref_metadata_2025 huggingface dataset:
The association between enteral feeding with survival of critical patients with COVID-19 patients
Authors: Gholamalizadeh Maryam , Salimi Zahra , Mobarakeh Khadijeh Abbasi , Mahmoudi Zahra , Tajadod Shirin , Moosavi Mehdi , Alami Farkhondeh , Bahar Bojlul , Doaei Saeid , khoshdooz Sara , Rahvar Masoume , Gholami Somayeh , Pourtaleb Masoume
Published: January 2024

Background: Coronavirus disease 2019 (COVID-19) results in several
complications and mortality in ICU patients. The effect of enteral
nutrition on the survival of COVID-19 patients in the ICU has been
investigated in limited instances. The aim of this study is to
investigate the association of enteral feeding with biochemical and
pathological indices associated with mortality in COVID-19 patients.
Methods: This case-control study was conducted on 240 patients with
COVID-19 hospitalized in the ICU including 120 eventually dead patients
as the cases and 120 discharged patients as the controls. All of the
patients received enteral nutrition. Data on general information,
anthropometric measurements, and the results of lab tests were
collected. Results: The recovered patients received significantly more
high protein (60.8% vs. 39.6%, P=0.004) and high volume (61.6% vs.
vs. 42.3%, P=0.005) formula compared to the dead patients. Mortality
was inversely associated with high volume (OR:0.45 CI95%, P=0.008) and
high protein formula (OR:0.42 CI95%, P=0.003). The results remained
significant after adjusting for age and sex. Further adjustment for
underlying diseases and smoking, BMI, and APACHII did not change the
results. Conclusion: The findings of the study showed that there was a
significant inverse association between mortality and high volume and
high protein formula in COVID-19 patients that warrants further
investigation.
Analysis of Clinical Features of Non-infectious Inflammatory Fever
Authors: LI Shan , LIAN Jianqi , ZHANG Yan , ZHOU Yun
Published: January 2024

Background: Fever of undetermined origin (FUO) is a challenging entity
with a striking presence in hospitals around the world. The main
etiologies are infectious, neoplastic, and non-infectious inflammatory
diseases. At present, the clinical characteristics of non-infectious
inflammatory diseases are less well described, the aim of this study is
to analysis the features of non-infectious inflammatory diseases, more
efficient differential diagnosis of FUO. Methods: A total of 444
patients with non-infectious inflammatory fever were retrospectively
studied, and another 133 patients with infectious fever act as control
group, to analysis the clinical features of non-infectious inflammatory
fever. Results: The pathogenesis of non-infectious inflammatory fever
were longer than infectious fever, and the levels of WBC, NEU, PLT, ESR,
LDH, α-HBDH, and FRT were increased obviously in non-infectious
inflammatory fever group, but the levels of RBC, HGB, LMY, and LCR were
dropped, and the positive rate of anti-nuclear antibodies, anti-RNP/Sm,
anti-ds-DNA, anti-Sm, anti-nucleosome, anti-histone, anti-ribosomal P
protein, anti-SSA, anti-SSB, and anti-Ro-52 antibodies in non-infectious
inflammatory fever patients were higher than infectious fever, the
concomitant symptoms of feeble, arthralgia, skin rash, and lower limb
edema were higher incidence in non-infectious inflammatory fever, but
the headache was lower incidence in non-infectious inflammatory fever,
the incidence of pericardial effusion was higher in non-infectious
inflammatory fever, but the incidence of pleural effusion was lower in
non-infectious inflammatory fever. Conclusion: The patients of
non-infectious inflammatory fever have longer pathogenesis, and the
levels of white blood cells, neutrophils, platelets, erythrocyte
sedimentation rate, lactate dehydrogenase, alpha-hydroxybutyrate
dehydrogenase, and ferritin were increased, the levels of red blood
cells, hemoglobin, lymphocytes, and lymphocyte to C-reactive protein
ratio were dropped, many autoantibodies were included, and the incidence
of feeble, arthralgia, skin rash, lower limb edema, and pericardial
effusion were higher, but the incidence of headache and pleural effusion
were lower.
Preschool children's heart rate variability across contexts of low and high emotional challenge correlates with their self-regulation performance
Authors: Clark Caron A. C. , Almeida Patricia Cardellini de , Joshi Keyoor
Published: January 2024

Heart rate variability (HRV) theoretically provides a biomarker for
self-regulation, although studies with young children offer mixed
findings regarding the relevance of emotional demands in this link. We
aimed to describe variation in children’s HRV during tasks with
relatively high and low emotional load, and to determine the relation of
HRV during these tasks to different behavioral measures of children’s
self-regulation. Electrocardiograms were recorded in 80 3 - 5-year-olds
(M = 57 months; 54% male, 47% female) while they completed a Go/No-go
task with low emotional load and an emotionally challenging Delay
Frustration task. Mean HRV was similar across these tasks, although it
increased during a between-task rest interval. Accounting for age,
gender, and caregiver education, higher HRV during both tasks, but not
during rest, correlated with children’s executive function task
performance. HRV during Delay Frustration correlated with
caregiver-reported self-regulation, whereas greater HRV withdrawal
during tasks correlated with children’s task-related negative
frustration. Children’s maintenance of HRV during emotional and
cognitive challenge may support their effective self-regulation.
Association of hypernatremia with mortality in patients with COVID-19: a systematic review and meta-analysis
Authors: Ma Yongzhi , 张 盼娟 , Hou Ming
Published: January 2024

Abstract Background: The purpose of this meta-analysis was to look at
the relationship between hypernatremia and mortality in COVID-19
patients. Methods: We searched the PubMed, Web of science, Embase and
Cochrane databases for articles published from the inception of the
database until August 27, 2022. Three researchers reviewed the
literature, retrieved data, and assessed the quality of the literature,
respectively. A meta-analysis was performed using State 17 software to
assess the value of the effect of hypernatremia on mortality in patients
with new coronavirus pneumonia. Results: A total of 9 publications was
finally included in this study, including a total of 11,801 patients
with COVID-19, including 1,278 in the hypernatremia group and 10,523 in
the normonatremia group. Meta-analysis showed that hypernatremia was
associated with mortality in patients with COVID-19 [OR = 4.15, 95%
CI (2.95-5.84), P = 0.002, I² = 66.7%] with a sensitivity of 0.36
[0.26, 0.48] and a specificity of 0.88 [ 0.83, 0.91]. The
posterior probability of mortality was 42% in patients with COVID-19
hypernatremia and 15% in patients who did not have COVID-19
hypernatremia. Conclusion: According to available data, hypernatremia is
associated with death in patients with COVID-19.
Assessment of the Effectiveness of National Park Policies and Laws in Promoting Biodiversity Conservation and Ecological Sustainability in Pakistan
Authors: Javed Banafsha , Ridwan Qamer , Huang Delin
Published: January 2024

Ecological sustainability and biodiversity conservation have become
crucial focal points for researchers and governments worldwide. In
response, Pakistan has implemented various laws and policies aimed at
enhancing forest sustainability and preserving its rich biodiversity.
Despite these efforts, little investigation has been conducted to assess
the effectiveness of these policies. The present study aimed to analyze
the impact of national park policies and laws on various factors,
including biodiversity conservation, water resource protection,
consumptive and non-consumptive benefits, research and education, and
the promotion of recreation and tourism in 19 National Parks of
Pakistan. A mixed-mode research design was utilized, combining both
quantitative and qualitative approaches. Data was gathered from 300
participants through a close-ended questionnaire employing a Likert
scale. Analysis of the collected data was conducted using the SMART PLS
4 software. The findings demonstrate that the existing policies and laws
have significantly contributed to the preservation of forest
sustainability in Pakistan. However, the study also revealed certain
limitations and gaps in the current policies, highlighting the need for
periodic re-evaluation and reform of ecological regulations. To foster a
performance-oriented and change-conscious public sector in the country,
there is a pressing demand for a more dynamic approach to ecological
policy-making. Continuous evaluation and adaptation of policies will be
essential in creating an environment conducive to sustainable
development, meeting the aspirations of stakeholders, and conserving
Pakistan’s valuable natural resources.
How I Feel About My School: Cross-cultural Invariance of a Child Self-report Questionnaire of School-based Well-being in the UK and Hong Kong
Authors: Katus Laura , Fink Elian , Wong Siu Ching , Wu Catherine , Dempsey Caoimhe , Leach Molly , D'Souza Hana , Hughes Claire
Published: January 2024

Well-being across the early years plays an important role for life-long
quality or life and learning. We examine the measurement invariance of a
school well-being measure (How I Feel About My School [HIFAMS])
across the UK and Hong Kong. In context of school closures during the
COVID-19 pandemic, we also assessed the measurement invariance across
cohorts tested online and in-person in both the UK and Hong Kong. We
found configural, metric and partial-scalar invariance across countries
and assessment modalities. Child HIFAMS scores were inversely related to
parental SDQ ratings in the UK, but not in Hong Kong. COVID-19
restrictions were strongly associated with HIFAMS in both the UK and
Hong Kong. Our results highlight the utility of HIFAMS across cultures
and assessment modalities, however contextual factors of time (e.g.,
COVID-19 restrictions and resulting feelings about school and peers) and
place need to be considered.
An Exploratory Study of Fintech Security Concerns among its Users in Nigeria
Authors: Agwi C. , J Akpojaro
Published: January 2024

The rapid growth of financial technology (Fintech) in Nigeria has
revolutionized the way people conduct financial transactions. However,
this technological advancement brings along a host of security concerns
that impact users’ trust and confidence in using Fintech platforms. This
exploratory study aims to investigate the security concerns among
Fintech users in Nigeria, shedding light on the underlying factors
contributing to these concerns. This research employs a mixed-methods
approach, combining qualitative interviews and quantitative surveys to
gather comprehensive data. The qualitative phase involves in-depth
interviews with a diverse sample of Fintech users, exploring their
perceptions, experiences, and concerns related to the security aspects
of Fintech services. The quantitative phase consists of a survey
administered to a larger sample, aiming to quantify the prevalence and
intensity of security concerns among Fintech users in Nigeria. The
results highlighted the importance of addressing data breaches, privacy
concerns, and authentication methods to alleviate user apprehensions.
Participants expressed a desire for more transparency regarding the
security practices of Fintech providers through improved communication
and education to enhance user awareness and understanding of the
security measures in place. This corroborates the notion that users’
trust in Fintech services is depends on their perception of the security
measures implemented by providers. The study’s contribute to the
existing literature on Fintech security, providing an in-depth
understanding of the specific security concerns faced by Nigerian users,
and identify potential strategies to enhance the security measures and
alleviate users’ concerns, which will ultimately foster greater adoption
and utilization of Fintech services.
The role and significance of SVIL protein in the occurrence and development of gastric cancer
Authors: Chen Meiqiong , Wang Ru , Cui Haihong , Zhang Rongshan
Published: January 2024

Aim: To discuss role and significance of SVIL protein in the occurrence
and development of gastric cancer. Materials and methods: Using GSE84437
as research object and analysis by bioinformatics. In vitro study, after
knockdown SVIL in MGC-803 and SGC-7901, observing cell biological
activities including proliferation, apoptosis, invasion and migration by
MTT, flow cytometry, transwell and wound healing assay. Results: By
bioinformatics analysis, SVIL was closely correlation with gastric
cancer, low expression group was significantly better compared with high
expression groups in survival rate (P＜0.001); in cell experiment, with
SVIL knockdown, MGC-803 and SGC-7901 cell proliferation were
significantly depressed with apoptosis significantly increasing
(P＜0.001), meanwhile, cells invasion and migration abilities were
significantly inhibited with SVIL knockdown in MGC-803 and SGC-7901 cell
lines (P＜0.001). Conclusion: SVIL overexpression might be closely
correlated with gastric cancer development.
CYBERBULLYING DETECTION: A COMPARATIVE STUDY OF CLASSIFICATION ALGORITHMS
Authors: NUTHALAPATI PARTHAV , ABBARAJU SRINIVAS ADITYA , VARMA G. HEMANTH , BISWAS SITANATH
Published: January 2024

In the realm of social media, cyberbullying’s pervasive impact raises
urgent concerns about its emotional and psychological toll on victims.
This study addresses the imperative of effectively detecting
cyberbullying. By leveraging ML and DL techniques, we aim to develop
reliable methods that accurately identify instances of cyberbullying in
social media data, enhancing detection efficiency and accuracy. This
facilitates timely intervention and support for affected individuals. In
this comprehensive analysis of existing systems, various ML and DL
models are extensively texted for cyberbullying detection. The evaluated
models include Random Forest, XgBoost, Naive Bayes, SVM, CNN, RNN, and
BERT. Pre-processed datasets are utilized to train and evaluate the
models. To evaluate the ability of each model to reliably identify
cyberbullying in social media data, performance metrics such as F1
score, recall, precision, and accuracy are used. The findings of this
study demonstrate the efficacy of different ML and DL models in
monitoring cyberbullying in social media data. Among the models
evaluated, the BERT model exhibits exceptional performance, achieving
the highest accuracy rates of 88 .8% for binary classification
and 86 .6% for multiclass classification.
Why Do Children Allocate More to the Poor? Impact Factors and Motivation in Children's Need-Based Fair Decision-Making
Authors: Peng Qingfeng , Li Mei , Li Hong
Published: January 2024

Fairness plays a pivotal role in moral development, and need-based
allocation, as a form of equitable resource distribution, entails
allocating resources to individuals with greater need. Children’s
need-based behavior is influenced by a variety of factors, including
situational, personal, and social aspects. Currently, the motivational
explanations for children’s need-based allocation are not fully
understood. Here, we present an explanatory model for comprehending
children’s motivation to allocate based on needs. We propose that
children’s motivation to engage in need-based behavior is a dynamic
process that unfolds in three stages: the balancing tendency stage (4-5
years old), the balancing-altruistic dual-motivation stage (6-7 years
old), and the altruistic tendency stage (8 years old and above). In
conclusion, we offer a perspective on future research directions aimed
at gaining a deeper understanding of the developmental process
underlying children’s fair decision-making.
Unconjugated Bilirubin as a State Marker in Patients with Schizophrenia in Acute Episode. An Egyptian Study
Authors: Mohamed Rehab Serag , Abd-Elsamei Afaf Mohamed , Gabry Dina Aly El , Mohamed Maha Sabry , Mohamed Mariam Yehia
Published: January 2024

Background: Evidence supporting a correlation between
unconjugated bilirubin and schizophrenia is considerable. Most previous
studies have reported a statistically relevant link between the two
variables. Aim of the Work: To assess the level of unconjugated
bilirubin in patients with schizophrenia in acute episode and to analyse
its correlation with the psychopathological, psychosocial and
neuropsychological dimensions. Patients and Methods: A sample
of 80 subjects with schizophrenia; divided into two major groups: Cases
(40 patients in relapse) and Controls (40 patients in remission) were
recruited. Subjects were diagnosed by DSM-IV and underwent the
Structured Clinical Interview for DSM-IV Axis I Disorders (SCID-I),
Positive and Negative Syndrome Scale (PANSS), General Assessment of
Function (GAF), and Trail Making Test (TMT) were used to assess severity
of symptoms, social functioning, and cognitive functioning respectively.
Subjects underwent a blood draw to measure serum bilirubin level. We
analysed the relation and correlation of unconjugated bilirubin with the
previous scales scores. Results: The study found that patients
with schizophrenia had significantly higher levels of total bilirubin,
and indirect bilirubin than healthy control subjects. Only one subject
(with schizophrenia) had an abnormally elevated total bilirubin level
(> 1.2 mg/dL). None of the subjects had a clinically
abnormal direct (> 0.3 mg/dl) or indirect bilirubin level
(> 1.2 mg/dL). A statistically significant positive
correlation was found between PANSS total score, PANSS N score, and
PANSS G score with total, direct, and indirect bilirubin levels. Linear
regression analysis showed that none of the variables entered into the
analysis (age, gender, smoking, BMI, Total PANSS, PANSS P, PANSS N,
PANSS G, GAF, TMT-A, TMT-B, duration of psychotic disorder, duration of
untreated psychosis, and antipsychotic medications) were predictive for
total or indirect bilirubin levels. However, Total PANSS, PANSS N, and
PANSS G were significantly predictive for direct bilirubin levels.
Conclusion: Our study managed to find a statistically
significant difference for total and unconjugated bilirubin mean serum
levels between patients with schizophrenia and healthy individuals.
However further longitudinal studies are needed to reveal the
contradictory results in literature on the unconjugated bilirubin and
Schizophrenia.
Integrating Smartwatches in Community Mental Health Services for Severe Mental Illness: A Case Series
Authors: Talbot Daniel , Johnston David , Foord Rachael , Casties Achim , Viaphay Nola , Tohamy Ahmed , Leeuwen Naomi Van , Sinclair Jodie , Harris Anthony
Published: January 2024

Objective: This case series explored the integration of
smartwatches in a community mental health service to support severe
mental illness (SMI) management and intervention. We examined whether
biometric data provided by smartwatches could help to predict relapse
and inform treatment decisions. Method: Four SMI patients were
selected from a prior study. Clinicians accessed patients’ biometric
data (activity, sleep, heart rate, and electrodermal activity) through
smartwatches. Results: Changes in circadian rhythm and
electrodermal activity preceded hospitalization in two cases.
Additionally, smartwatch data was effectively used to guide targeted
interventions, improving patient treatment outcomes.
Conclusion: Integrating smartwatches in community mental health
services offers promise as adjunct tools for severe mental illness
management. However, ethical considerations on data privacy and
technology reliance require further evaluation.
Advancement in Efficient Approaches for Detection of Prevalent Phishing Attacks
Authors: Kaur Hargeet , Pandya Kaxit , Kapaliya Sarthak , Modi Saloni
Published: January 2024

Phishing attacks are thoroughly engineered attacks where the
attackers use emails, messages, and websites of reputed sources as a
medium to trick their targets into sharing sensitive content. This
sensitive content primarily consists of their financial information, in
the case of small attacks whereas some planned advanced attacks also
target to obtain their login information. In the past few years, there
has been a noticeable shift in attackers’ priorities, moving away from
targeting individuals and instead concentrating on the organization’s
employees. It is also observed that most cyber-attacks are the result of
employee negligence. Due to the widespread availability of phishing kits
and the expansion of ransomware as a service (RaaS), aspiring hackers
now possess a straightforward method to defraud individuals. What is
particularly worrisome about this growing trend is that individuals
lacking technical expertise are engaging in such activities using simple
tools and online instructional materials. Machine learning can help in
recognizing different phishing attacks and patterns. We describe several
classical algorithms to detect Phishing attacks. We aim to utilize
machine learning techniques like Multilayer perceptron, Random Forest,
XG Boost, and different classifiers for detection. This paper will
compare various studies for detecting phishing attacks using each
Artificial Intelligence technique: Deep Learning and Machine learning.
In order to enhance our study, we have also listed various other
conventional methods of detection that do not utilize the benefits of
training through machine learning models.
Mass spectrometric identification of the secretome of Plasmodium falciparum merozoites to provide novel candidates for vaccine development pipeline
Authors: Munjal Akshay , B Rex D. A. , Prasad T. S. Keshava Prasad , Mukherjee Soumyadeep , Pati Soumya , Singh Shailja
Published: January 2024

Purpose: Merozoites are the only extracellular form of blood stage
parasites, making it a worthwhile target for vaccine and drug
development. Multiple invasins that are stored in the merozoite apical
organelles, are secreted just prior to invasion, and mediates its
interaction with host cell. A comprehensive identification of all these
secreted invasins is lacking and this study addresses that gap.
Experimental design: Pf3D7 merozoites were enriched and were triggered
to discharge apical organelle contents by exposure to ionic conditions
mimicking that of blood plasma. The secreted proteins were separated
from cellular contents and both the fractions were subjected to
proteomic analysis. Also, the identified secreted proteins were
subjected to gene ontology and PPI network analysis. Results: A total of
63 proteins were identified in the secretory fraction with membrane and
apical organellar localization. This includes various MSPs, micronemal
EBAs, various rhoptry bulb proteins, which plays a crucial role in
initial and late attachment of the merozoite with the RBC, and harboured
multiple linear B cell epitopes. Conclusion and clinical relevance: We,
for the first time, report the secretory repertoire of merozoite. This
information can be utilized to develop better invasion blocking
multisubunit vaccines, comprising of immunological epitopes from several
secreted invasins.
Cyanotis shrirangii (Commelinaceae), a new species from lateritic plateaus of Konkan region of Maharashtra, India
Authors: Chandore Arun , Borude Devidas , Bhalekar Paresh
Published: January 2024

A new species Cyanotis shrirangii is described from Konkan region of
Maharashtra, India. The new species is allied with Cyanotis pilosa
Schult. & Schult.f. but differs in having annual habit, stems densely
hispid hairy, leaves distichous and hispid on both sides; flowers
purple, 2–3-flowered cincinni, anthers black; seeds ovoid to pyramidal
and rugose deeply pitted.
Catalyst- and Additive-Free Hydrosulfonylation of 1, 3-Dienes for Allylic Sulfones Synthesis
Authors: Zhang Shuaichen , Chang Xiaoqiang , Ren Yu , Zhang Jing , Sun Peng
Published: January 2024

The demand for environmentally-friendly processes in organic synthesis
has spurred interest in developing catalyst- and additive-free reactions
for the synthesis of valuable organic compounds. Among these, allylic
sulfones are a crucial structural motif used in drug development and
organic synthesis. Despite several catalytic protocols have been
established for the synthesis of allylic sulfones in recent years, the
catalyst- and additive-free process has yet to be explored. In this
study, we report a highly efficient and green pro-tocol for the
synthesis of allylic sulfones from aryl-1, 3-dienes and sulfinic acids.
The reaction was conducted under mild conditions using an aerobic
atmosphere at room temperature without any catalyst or additive. The
procedure demonstrated excellent atom economy, good regio- and
chemo-selectivity, simple operation, and scalability. Overall, this
method provides a promising strategy for the synthesis of allylic
sulfones in a sustainable and cost-effective manner.
Selective adsorption of DMSO on two chlorinated calixarene type scaffolds
Authors: Baa Ebenezer , Krause Rui , Watkins Gareth , Smith Vincent
Published: January 2024

The selective adsorption of DMSO vapor on calixarene scaffold from a
mixture consisting of either DMSO and water or DMSO and methanol was
studied. Two calixarene-type scaffolds based on 4-chlorobenzaldehyde
were obtained by reacting the aldehyde with either resorcinol or
pyrogallol and characterized by various techniques including NMR, FTIR,
thermal, and XRD. These materials were then used as sorbent materials
for separating vapors. Results from this investigation indicate the
scaffolds show selectivity towards DMSO. The resorcinol-based scaffold
showed a higher selectivity for DMSO compared to the pyrogallol
counterpart. The influence of synthesis solvent on the conformation and
structural changes resulting from the interaction of the guest molecules
with the host was also investigated.
Early Perspective Taking predicts later Cognitive Flexibility: A longitudinal study
Authors: Kloo Daniela , Kaltefleiter Larissa J. , Sodian Beate
Published: January 2024

Perspective taking and cognitive flexibility are important abilities for
navigating our everyday lives. In this longitudinal study with 108
children (61 girls, mostly White), we investigated the developmental
relation between Level 1 perspective taking at 27 months of age and
Level 2 perspective taking at 52 months of age as well as relations to
cognitive flexibility at 52 months of age. We found that early
perspective taking was significantly related to later, more complex
perspective taking abilities as well as to cognitive flexibility. This
highlights the importance of early perspective taking abilities for
later perspective understanding and flexible cognition.
Coparenting moderates the association between maternal prenatal depressive symptoms and infant regulatory capacity
Authors: Pinto Tiago Miguel , Feinberg Mark Ethan , Figueiredo Barbara
Published: January 2024

As a development-enhancing or a risk-promoting environment, coparenting
may shape the association between maternal prenatal depressive symptoms
and infant regulatory capacity. This study aimed to analyze the
moderator role of positive and negative coparenting in the association
between maternal prenatal depressive symptoms and infant regulatory
capacity at 3 months. A sample of 103 primiparous couples (N = 206
parents) was recruited at the 1st trimester of pregnancy. Mothers
reported on depressive symptoms at the 1st trimester of pregnancy, and
both parents reported on depressive symptoms, coparenting, and infant
regulatory capacity at 2 weeks and 3 months postpartum. Higher levels of
maternal prenatal depressive symptoms and higher levels of maternal and
paternal reports of negative coparenting at 2 weeks postpartum were
associated with lower infant regulatory capacity at 3 months. Negative
coparenting at 2 weeks postpartum accentuated the association between
maternal prenatal depressive symptoms and infant regulatory capacity at
3 months. The results support a view of negative coparenting as a
risk-promoting environment that can accentuate the association between
maternal prenatal depressive symptoms and infant regulatory capacity.
Infants in families with mothers with elevated prenatal depressive
symptoms and with high levels of negative coparenting may be at high
risk of low regulatory capacity.
Constitutive G protein coupling profiles of understudied orphan GPCRs
Authors: Lu Sumin , Jang Wonjo , Inoue Asuka , Lambert Nevin
Published: January 2024

Background and Purpose: A large number of GPCRs are potentially valuable
drug targets but remain understudied. Many of these lack well-validated
activating ligands and are considered “orphan” receptors, and G
protein coupling profiles have not been defined for many orphan GPCRs.
Here we asked if constitutive receptor activity can be used to determine
G protein coupling profiles of orphan GPCRs. Experimental Approach: We
monitored GDP-sensitive interactions (coupling) between 48 understudied
orphan GPCRs and five G proteins (240 combinations) using
bioluminescence resonance energy transfer (BRET). No activating ligands
were used. Constitutive BRET between the same receptors and β-arrestins
was also measured. Key Results: We found sufficient GDP-sensitive BRET
to generate G protein coupling profiles for 22 of the 48 receptors we
studied. Altogether we identified 48 coupled receptor-G protein pairs,
many of which have not been described previously. Conclusion and
Implications: We conclude that receptor-G protein complexes that form
spontaneously in the absence of guanine nucleotides can be used to
profile G protein coupling of constitutively active GPCRs. This approach
may prove useful for studying G protein coupling of other GPCRs for
which activating ligands are not available.
Current status and influencing factors of evidence-based practice with knowledge, attitude and practice of ophthalmic nursing staffs in South China
Authors: Xiaomin Fang , Huiming Xiao , Yu Zhang , Guiting Wang , Shanshan Luo
Published: January 2024

ABSTRACT Objective: To investigate the current status and influencing
factors of evidence-based practice (EBP) with knowledge, attitude and
practice (KAP) of ophthalmic nursing staffs in South China. Methods:
Using a convenient sampling method, we selected 429 ophthalmic nursing
staffs from 28 ophthalmology specialist hospitals or general hospitals
in South China, and investigated their general information and
implemented the evidence-based practice questionnaire (EBPQ). Results:
The scores of EBP and KAP of ophthalmic nursing staffs in South China
from high to low were as follows: practical attitude (4.85±1.07) points,
practical behavior (4.42±1.14) points, practical knowledge and skills
(4.30±0.65) points. The single factor analysis results showed that the
first graduation degree, technical title, scientific research
achievements, whether or not participate in EBP training, the frequency
of reading literature at ordinary time, and whether or not participate
in EBP project were the influencing factors of EBP level; the
multi-factor analysis results showed that EBP attitude, EBP knowledge
and skills, whether or not participate in EBP training were independent
influencing factors of EBP. Conclusions: The ophthalmic nursing staffs
in South China have a positive attitude towards EBP, however, their EBP
knowledge, skills and behavioral capabilities need to be focused and
improved. The ophthalmic nursing administrators should fully master the
factors affecting the implementation of EBP, and take effective
intervention measurement to improve the EBP abilities of ophthalmic
nursing staffs, and promote the development of EBP in ophthalmology
specialty.
Solutions of nonlinear time fractional Klein-Gordon equations using composite fractional derivatives
Authors: Kachhia Krunal , Prajapati Jyotindra
Published: January 2024

In this paper, the generalized iterative method has been successfully
implemented for nding exact solutions of the non linear time fractional
Klein-Gordon equations using com- posite fractional derivatives.
Graphical representations also been studied. Illustrations related to
Klein-Gordon equations using composite fractional derivative viz.
Riemaan- Liouville fractional derivatives, Hilfer fractional
derivatives, Caputo fractional derivatives has been discussed.
ECHOCARDIOGRAPHIC DIAGNOSIS OF AORTIC SINUS ANEURYSM RUPTURE INTO THE RIGHT ATRIUM WITH TRICUSPID VALVE PROLAPSE AND SEVERE REGURGITATION：A CASE REPORT
Authors: qiang ma , ZHANG Xia
Published: January 2024

Sinus of Valsalva aneurysm (SVA) is a relatively rare organic cardiac
anomality. Here, we report a case of a ruptured SVA with tricuspid valve
prolapse (TVP) and severe tricuspid regurgitation (TR). A 33-years-old
man was admitted to our hospital who presented chest tightness after
activity. Coronary sinus aneurysm(1.2cm*1.0cm) and tricuspid systolic
prolapsed into the right atrium was detected by echocardiography. And
later the patient was confirmed by surgery and pathology that the
congenital aortic sinus aneurysm ruptured. Echocardiography played a
vital role in the diagnosis and differential diagnosis of this case of
RSVA and even the follow-up preoperative preparation.
Altered Sphingolipid metabolism in Vernal Keratoconjunctivitis: Newer Insights
Authors: Vignesh Menta , Agarwal Shweta , Das Ujjwalkumar , Moksha Lakshmi , Srividya Gurumurthy , Anandan Amrutha Mahalakshmi , Srinivasan Bhaskar , Iyer Geetha , Velpandian Thirumurthy , Angayarkanni Narayanasamy
Published: January 2024

Background: The etiopathogenesis of vernal keratoconjunctivitis (VKC),
is incompletely understood. Bioactive lipids play a key role in the
allergic disorders and there are no studies exploring it in the ocular
surface allergic disorder such as VKC. This study focused on the
sphingolipid metabolism in VKC and explored the association of ocular
surface sphingolipids with the refractory nature of the disease.
Methods: Active VKC cases and age-matched healthy controls were
recruited as part of a one-year prospective study at our tertiary eye
care centre in South India. Imprint cytology was used to assess gene
expression of enzymes of sphingolipids metabolism in conjunctival cells.
Sphingolipids levels were estimated in the tears by LC-MS/MS analysis.
Systemic levels of Sphingosine-1-phosphate (S1P) and
Ceramide-1-Phosphate and IgE were estimated by ELISA. IgE induced
regulation of S1P receptor gene expression was assessed by western blot
analysis of histone deacetylases in cultured mast cells. Results:
Significantly altered gene expression of the sphingolipids enzymes was
noted in VKC in conjunctival cells. Pooled tears showed significantly
lowered levels of S1P (d17:1), S1P (d17:0) and S1P (d20:1) in VKC.
Additionally, Cer (d18:/17:0) was significantly lowered in R-VKC
compared to NR-VKC. Lowered C1P and raised IgE were observed at serum
level in VKC. Increased S1P receptors and histone deacetylases
expression indicate regulation by histone modification as evaluated in
IgE induced mast cells. Conclusion: Altered sphingolipid metabolism is
associated with VKC pathogenesis and is differential in refractory cases
that showed lower ceramide and specific sphingosines compared to
non-refractory VKC.
HEPATOBLASTOMA TREATED SUCCESSFULLY WITH DRUG-ELUTING BEAD TRANSARTERIAL CHEMOEMBOLIZATION: OUTCOME OF SEVEN-YEAR FOLLOW-UP
Authors: yağcı-küpeli begül , Ballı Tuğsan
Published: January 2024

Hepatoblastoma, the most common liver tumor of childhood is generally
treated with chemotherapy and surgery. More than half of the patients
are unresectable at time of diagnosis. Therefore, neo-adjuvant
chemotherapy has been the mainstay of treatment. However, long-term
effects of systemic chemotherapy and inadequate response to conventional
chemotherapy have been major issues in management of hepatoblastoma. As
a result, attempts towards local treatment options were made such as
transarterial chemoembolization (TACE) which allows tumor necrosis both
via vascular occlusion and chemotherapy. Here we present a seven-year
follow-up results of an infant treated with neoadjuvant chemotherapy,
drug-eluting bead-TACE and surgery.
Three novel avastroviruses identified in dead wild crows
Authors: Zhang Chunge , Yang Yongchun , Hu Tao , Zhou Hong , Zhang Cheng , Cao Jian , Li Juan , Wang Peihan , Wong Gary , Wang Xiaodu , Song Houhui , Gao George F. , Shi Weifeng , Bi Yuhai
Published: January 2024

We described three novel avastroviruses identified in dead wild crows by
next-generation and Sanger sequencing. They shared 40-50% nucleotide
identities to known avastroviruses, and formed a new cluster in the
genus Avastrovirus. This study highlighted the continually expanding
host range and increasing genetic diversity of avastroviruses.
Longitudinal profile of sHLA-G during pregnancy and its association with small for gestational age births in North Indian pregnant females: A nested case-control study
Authors: Sehgal Shilpi , Vyawahare Sushrut , Bhatnagar Shinjini , Kshetrapal Pallavi , group GARBH-Ini study
Published: January 2024

Abstract Objective: To assess the difference in the trajectories of
soluble HLA-G in maternal sera during pregnancy between women delivering
small for gestational age (SGA) and appropriate for gestational age
(AGA) neonates. Design and Settings: Pilot case-control study nested
within a cohort study - Garbh-Ini. Population: North-Indian pregnant
females delivering SGA (N=23) or AGA (N=17) neonates. Method: Soluble
HLA-G1/G5 was estimated in the maternal sera at different time points in
pregnancy using sandwich ELISA. Linear mixed models were built and
compared to study the association between sHLA-G levels during pregnancy
and SGA births. Main Outcome Measure:Birth of SGA or AGA neonates.
Results: No significant difference was observed in the sHLA-G
trajectories during pregnancy in mothers delivering SGA as compared to
those delivering AGA (p-value = 0.5677). A trend towards higher sHLA-G
levels at the first trimester of pregnancy (<14weeks of
gestation) was observed in mothers delivering SGA neonates (Median=
41.71, IQR= 21.31 to 71.38) as compared to those delivering AGA neonates
(Median=37.58, IQR=19.05 to 73.57). Conclusion: The trajectory of sHLA-G
during the course of pregnancy is not different between mothers
delivering SGA and those delivering AGA. However, a trend towards higher
sHLA-G levels at the first trimester was observed in mothers delivering
SGA, which could be explored further in studies with larger sample
sizes. Funding: “Department of Biotechnology, Ministry of Science and
Technology, Government of India (BT/07/IYBA/2013-12), (grant
BT/PR9983/MED/97/194/2013)” and “Grand Challenges India–All Children
Thriving Program, Biotechnology Industry Research Assistance Council
(grant BIRAC/GCI/0114/03/14-ACT)”. Keywords: India, SGA, pregnancy,
sHLA-G
RHINOSINUSITIS IN POST-RENAL TRANSPLANT PATIENTS- a Diagnostic Conundrum and Treatment Dilemma
Authors: Baruah Shruti , Ojha Tarun
Published: January 2024

INTRODUCTION: Immunosuppressed Renal transplant patients are often
susceptible to upper airway infections, especially those of the
paranasal sinuses. The increasing number of cases of rhinosinusitis by
opportunistic organisms in post-renal transplant (PRT) patients has
spawned our interest to analyze this subset of patients. MATERIAL &
METHOD: A Retrospective observational Study was done on all PRT patients
who presented to the Dept. of Otorhinolaryngology, MGH, Jaipur, with
symptoms of rhinosinusitis and required hospitalization. The following
variables were studied: age at time of transplantation, gender, cause of
CRF, Pre-transplant co-morbidities, Post-transplant immunosuppressant
medications, time-interval between transplant and development of
symptoms of rhinosinusitis, presenting complaints, radiological
presentation, treatment approaches and surgical challenges experienced.
RESULT: Out of 11 patients, 8 were males (72.7%) and 3 females
(27.3%). All the patients (100%) of the study group presented with
unilateral facial pain and/or headache in about 6-12 months post-renal
transplant. The most consistent CT finding was involvement of the
Maxillary sinuses. All the cases worsened rapidly and required immediate
treatment and 9(81.8%) of these patients required Endoscopic surgical
debridement, of which 3 (27%) cases required revision surgery within 2
weeks. CONCLUSION: In the immunocompromised host, the initial
presentation of rhinosinusitis may be subtle, but the subsequent
clinical course is usually fulminant or even fatal. Therefore, their
pre-transplant ENT evaluation, a high index of suspicion in the
post-transplant period and timely management is vital to avoiding fatal
outcomes in these patients. Keywords: Rhinosinusitis, Fungal infections,
Invasive Fungal Sinusitis, Immunocompromised states, Post-renal
transplant infections. Succinct Keywords: Fungal Rhinosinusitis,
Opportunistic infections, Post-transplant Sinusitis, Chronic
Rhinosinusitis, Mucormycosis.
Biological Prescience: The Role of Anticipation in Organismal Design
Authors: Deans Carrie
Published: January 2024

Anticipation is the act of using information about the past and present
to make predictions about future scenarios. As a concept, it is
predominantly associated with the psychology of the human mind; however,
there is accumulating evidence that diverse taxa without complex neural
systems, and even biochemical networks themselves, can respond to
perceived future conditions. Although predictive biological responses,
such as circadian rhythms and stress preconditioning, have been
extensively studied over the last century, newer research on predictive
genetic networks in microbes have highlighted the existence of
anticipatory processes in older, more primitive taxa. When taken
together, these seemingly disparate processes can be connected based on
a shared anticipatory design. This review discusses the important and
often overlooked role of anticipation in the evolution of biological
designs, and advocates for establishing a fundamental distinction
between anticipatory and reactive processes as an effort to advance our
understanding of complex biological processes.
Benefits of prophylactic emicizumab in enhancing immune tolerance induction in a hemophiliac boy with very high inhibitor titer
Authors: Sirachainan Nongnuch , Chuansumrit Ampaiwan , Parapakpenjune Surapan , Wongwerawattanakoon Pakawan , Lertthammakiat Surapong , Kadegasem Praguywan , Sasanakul Werasak
Published: January 2024

A 12-year-old severe hemophilia boy had developed inhibitor since he was
two. At 10 years of age, he received high dose factor VIII after plasma
exchange in addition to bypassing agents for treatment of CNS bleeding,
causing peak inhibitor titer of 3,360 BU. ITI was initiated when his
inhibitor was decreased to 140 BU, and the prophylactic emicizumab had
been added since the second year of ITI. After 3.5 years of ITI and
emicizumab, his inhibitor titer was down to 0.56 BU (factor VIII
recovery was 60% and half-life 5.5 hours). His QoL was remarkedly
improved and without bleeding.
Esophageal Thermal Injury Secondary to Cryoballoon Ablation for Atrial Fibrillation: A Case Report
Authors: Er-hong Zu Ke-la Tu , Lu Yan-mei , zhou xianhui , Li Yao-Dong , Zhang Jiang-Hua , Xing Qiang , Tang Baopeng
Published: January 2024

The esophageal injury after cryoballoon ablation is much rarer than that
after radio-frequency ablation.Although it‘s rare, once progressed into
atrioesophageal fistula, it could cause a catastrophic iatrogenic
tragedy.We report on the occurrence of esophageal injury of A
71-year-old female with symptomatic paroxysmal AF who underwent
cryoballoon pulmonary vein isolation. The patient complaint about chest
pain exacerbated by swallowing on the postoperative day. Endoscopy
detected the esophageal thermal lesions. After 7 days of interventions,
the symptoms relieved and retest of endoscopy showed normal esophageal
mucosa.We suggest that esophageal protection in cryoballoon ablation is
just as important as in RF ablation.
The new stochastic solutions to the perturbed NLSE
Authors: Abdelwahed Hesham , abdelrahman mahmoud
Published: January 2024

This article extracts exact solutions for the perturbed nonlinear
Schrödinger’s equation (PNLSE) with Kerr law nonlinearity forced by
multiplicative noise in It^o sense by utilizing the unified solver
method. The presented solutions involve three types: rational function,
trigonometric function and hyperbolic function solutions. These
stochastic solutions may be applicable for investigation various complex
phenomena in applied science and new physics. We exhibit the influence
of multiplicative noise on the solution of the PNLSE forced by
multiplicative noise in It^o sense. The study and acquired solutions
clarify that the unified solver technique is sturdy and efficient.
Finally, some 3D proles to some of the gained solutions are also
illustrated.
Genetic diversity and population structure of Jubaea chilensis (Molina) Baill., an endemic and monotype gender from Chile, based on SNPs markers
Authors: Jara-Arancio Paola , Carvalho Carolina , Bustamante Ramiro O. , Carmona-Ortiz Martín , Villela Priscilla Schmidt , Andrade Sonia , Peña-Gómez Francisco , González Luis Alberto , Fleury Marina
Published: January 2024

Jubaea chilensis (Molina)Baill., also named Chilean palm, is an endemic
species found in the coastal area of Mediterranean sclerophyllous forest
in Chile. It has a highly restricted and fragmented distribution along
the coast, being under intense exploitation and anthropogenic impact.
Based on 1,038 SNP markers, we evaluated the genetic diversity and
population structure among six J. chilensis natural groups. We observed
low levels of genetic diversity (overall HE = 0.024 and HO =0.014), a
deficit of heterozygotes, and high levels of inbreeding (mean FIS =
0.424), with little or no random mating. All Wright fixation index and
Nei’s genetic distance pairwise comparisons indicated moderate
differentiation among populations, with a tendency to similarity. There
was no evidence of isolation by distance (r =-0.214, P =0.799). In the
cluster analysis, we observed a closer relationship among Culimo,
Cocalán and Candelaria populations. The K value that best represented
the spatial distribution of genetic diversity was ∆K =3. Habitat
fragmentation and deterioration of the sclerophyllous forest may have
driven inbreeding and low levels of genetic diversity in the palm groves
of J. chilensis, putting the persistence of present and future
populations at risk. In this scenario, it is imperative to reclassify J.
chilensis as an endangered species, as well as a Natural Monument, in
order to improve conservation efforts, the species management, and the
environmental protection Also, the preservation of genetically different
individuals may increase the overall genetic variability required to
sustain the species persistence in the context of climate change and
anthropogenic disturbance.