bluuebunny/crossref_metadata_2025 huggingface dataset:
EVALUASI PROGRAM BURSA KERJA KHUSUS (BKK) SMK DI KABUPATEN BEKASI
Authors: Sumarno Sumarno
Published: June 2020

Setiap SMK di Kabupaten Bekasi memilki Bursa Kerja Khusus (BKK). Fungsi utama BKK adalah menyalurkan tenaga kerja lulusannya. Evaluasi terhadap program ini menggunakan model kesenjangan (discrepancy) dari Provus. Pelaksanaannya terdiri dari 4 tahapan, yaitu: Desain, Instalasi, Proses, dan Produk. Penelitian ini menggunakan metode kualitatif yang menghasilkan data deskriptif. Tujuan penelitian ini untuk mengetahui kesenjangan antara standar yang ditetapkan dengan fakta aktual yang terjadi. Data dikumpulkan dengan instrumen angket, wawancara, dokumentasi, dan observasi. Analisis data menentukan setiap komponen dengan kategori sangat tidak sesuai, tidak sesuai, sesuai, dan sangat sesuai. Kesenjangan tertinggi tahap desain terdapat pada aspek pedoman penyelenggaraan, sumber daya pada tahap instalasi dan pelaporan pada tahap produk, hal ini terjadi karena pengurus BKK tidak mengetahui petunjuk teknis akibat kurangnya sosialisasi dari Disnaker setempat. Sosialisasi tidak optimal karena kurangnya tenaga Pembina/pemandu dari instansi ketenagakerjaan. Aspek paling baik tingkat kesesuaiannya ada pada tahap proses, hal ini disebabkan beberapa kegiatan terintegrasi dengan kegiatan PKL. Rata-rata hasilnya adalah sesuai standar, keputusannya program dilanjutkan dengan rekomendasi berupa perbaikan pada beberapa komponen.
SAĞLIĞIN KORUNMASI VE GELİŞTİRİLMESİNDE BİREYLERİN SAĞLIKLI YAŞAM BİÇİMİ DAVRANIŞ DÜZEYİNİN BELİRLENMESİ
Authors: DEĞERLİ Havvana , YİĞİT Arzu
Published: June 2020

Bu çalışmanın amacı; sağlığın korunması ve geliştirilmesinde bireylerin sağlıklı yaşam biçimi davranış düzeyini ölçmektir. Yaşam biçimi; bireyin alışkanlıkları, tutumları ve değerlerinin toplamıdır. Sağlıklı yaşam biçimi ise bireylerin sağlık durumu üzerinde etkisi olan ve bireylerin kontrolü altında olan tüm davranışlardır. Bu davranışlar, bireyin sağlığını korumasını ve geliştirmesini sağlayan yaşam biçiminin bir göstergesidir. Araştırmanın evrenini Isparta il merkezindeki 18 yaş ve üzeri bireyler oluşturmaktadır. Örnekleme yöntemi olarak tesadüfi olmayan örnekleme yöntemlerinden olan “kolayda örnekleme yöntemi” kullanılmıştır. Araştırmada veri toplama aracı olarak Sağlıklı Yaşam Biçimi Davranışları Ölçeği II” (SYBDÖ-II) kullanılmıştır. Araştırmada 510 kişinin verisi değerlendirilmeye tabi tutulmuştur. Anket formları vasıtasıyla toplanan verilerin bilgisayar ortamına aktarılmasında Statistical Package for the Social Sciences (SPSS 22.0) programı kullanılmıştır. Elde edilen verilerin analizinde bu programdan faydalanılmıştır. Sağlıklı Yaşam Biçimi Davranışları Ölçeğinden “iyi” puan alınması sağlıklı yaşam biçimi davranışlarına dikkat edildiği ve olumlu sağlık davranışları sergilendiği anlamına gelmektedir. Araştırma sonucunda katılımcıların ölçekten 116,01 puan aldıkları ve “orta” düzeyde olumlu sağlık davranışı sergilediği tespit edilmiştir. Araştırma sonuçlarından hareketle, bireylerin sağlıklı yaşam biçimiyle ilgili davranışlar hakkında bilgilerini artıracak ve geliştirecek eğitim programlarının oluşturulması ve uygulanması önerilmektedir.
TÜKETİM TOPLUMUNDA META-MARKA BAĞIMLILIĞININ GÖRÜNÜMLERİ
Authors: ÖZKUL Metin , YILMAZ UÇAK Emine
Published: June 2020

Bu çalışma, tüketim toplumu bağlamında metayı, yüklendiği anlamlarıyla ele alarak, marka bağımlılığı ile olan ilişkisine açıklık getirmeye çalışmakta ve marka bağımlılığının gündelik yaşam pratiklerine nasıl yansıdığını tartışmaktadır. Tüketim toplumu kavramlaştırması, bir yönüyle Marxist bir eleştirinin konusu olarak ortaya çıkmış, diğer yönüyle ise Marxist toplum eleştirisinin de birçok argümanlarını kullanmıştır. Bu çalışma literatüre yansıyan bulgular ve düşünceler çerçevesinde günümüz toplumunda marka tüketiminin toplumsal ilişkilerdeki işlevlerini tartışmaktadır. Çalışma, her geçen gün daha fazla yükseliş gösteren tüketim eğilimleri, bireylerin kendilerini toplumsal alana sunmaları, aldıkları tepkileri ve bu sınırlar içinde oluşan ilişkilerin anlamsal çerçevesini çizmeye çalışmaktadır. Ulaşılan sonuçlar açısından; tüketim toplumunda, ürünlerin kullanım değerleri farklılaşmakta ve ortaya çıkan yeni kullanım değerleri, her ürünün başka bir boyutunu ortaya koymaktadır. Ürünün kimliği olan markalar, tüketim nesnesini putlaştırarak; nesneye toplumsal itibar kazanma ya da kaybetme misyonu yüklemektedir.
Respuesta de Aedes aegypti L. (Diptera: Culicidae) a transflutrina y linalol impregnados en diferentes tipos de tela
Authors: Paiz-Moscoso Keila Elizabeth , Fernández-Salas Ildefonso , Grieco John Paul , Achee Nicole L , Torres-Estrada José Luis
Published: June 2020

Objetivo. Evaluar el efecto de repelencia espacial contra Ae. aegypti de dos compuestos químicos impregnados en diferentes tipos de telas. Material y métodos. El estudio se realizó en el periodo 2015-2016 en el Centro Regional de Investigación en Salud Pública, del Instituto Nacional de Salud Pública. Se utilizó el Sistema de Procesamiento de Alto-rendimiento para evaluar la respuesta de Ae. aegypti a transflutrina y linalol, impregnados individualmente a di­ferentes concentraciones en popelina, algodón y poliéster. También se determinó el efecto de sus mezclas, lavado sobre la residualidad y porcentaje de protección. Resul­tados. La mayor respuesta de repelencia espacial fue para el tratamiento linalol-algodón al 0.1% (RE= 70 ± 5.77%). La mezcla de linalol 0.1% y transflutrina 0.001% presentó un porcentaje de repelencia espacial similar para los tres tipos de tela. El tratamiento transflutrina-popelina 0.001% mantuvo una residualidad de cinco días. El linalol al 0.1% produjo 62.50% de protección en presencia de un estímulo de atracción. Conclusión. Se sugiere la impregnación de linalol al 0.1% en ropa como medida de protección de las picaduras de Ae. aegypti.
Allocating medical resources fairly: the CSG bioethics guide
Authors: Macklin Ruth
Published: June 2020

On April 12, 2020, a bioethics guide for allocating scarce hospital resources during the current Covid-19 pandemic was posted on the website of the Consejo de Salubridad General (CSG) of the Government of Mexico. The guide, entitled Guía bioética para asignación de recursos limitados de medicina crítica en situación de emergencia, was intended as a preliminary document, but the website posting did not describe it as a first step in the process. The publicity resulted in a wide array of comments and criticisms. That first version posted on the CSG website contained an age-based criterion for breaking a tie between two or more medically eligible patients who needed of a ventilator: younger patients would have prefer­ence over older ones. The final version of the guide eliminated that criterion and instead, relied on the leading public health principle, “save the most lives”, without regard to personal characteristics other than the possibility of benefitting from the scarce medical resources.
iTRAQ-based quantitative proteomic of tobacco (Nicotiana tabacum L.) identified major host proteins involved in photosystems throughout the aging process
Authors: He Conglian , Sun Shubin , Tang Yun , He Chenggang , Li Pengfei , Xu Tianyang , Zhao Gaokun , Zou Congming , Lin Zhonglong , Chen Yi
Published: June 2020

The authors have withdrawn this preprint due to author disagreement.
Dezocine inhibits cell proliferation, migration and invasion by targeting CRABP2 in ovarian cancer
Authors: Zhang Chuanfeng , Pan Ruirui , Ma Shuangshuang , Xu Shoucai , Wang Baosheng
Published: June 2020

Background: Previous studies have shown that some anesthesia drugs can inhibit tumor growth and metastasis. As a clinical anesthetic drug, dezocine has been reported to play an important role in immune function. However, the effects of dezocine on ovarian cancer cell growth and metastasis are not fully understood. Results: In this study, we found that dezocine dose-dependently inhibited the viability of ovarian cancer ES-2 and SKOV3 cells. Dezocine suppressed the migration and invasion abilities of ovarian cancer cells and promoted apoptosis. Moreover, the Akt/mTOR signaling pathway was also inhibited by dezocine. Furthermore, the mechanism study showed that dezocine could significantly inhibit the expression of CRABP2, and CRABP2 overexpression reversed the inhibitory effects of dezocine on ovarian cancer cell proliferation and migration. Conclusions: In conclusion, dezocine has significant anti-tumor effects on the growth and metastatic potential of ovarian cancer cells, and CRABP2 functions as a downstream effector of dezocine.
Exploring the Effective Factors on Fires Related Injuries in Residential Buildings Inhabitants: Findings from a Paradigm Model
Authors: Shokouhi Mohammadreza , Nasiriani Kadijeh , Ardalan Ali , Khorasani-Zavareh Davoud
Published: June 2020

Background

Fires is one of the most important hazards leading to human casualties as well as financial losses in the world, mostly in low- and middle- income countries. This study aims to provide in-depth information on fires in residential buildings and explain the safety process against fires in Iran.
Methods

This study has a qualitative study design using a grounded theory principle, recommended by Strauss and Corbin, and using the Paradigm Model. The data were collected via in-depth interviews with 27 participants, including injured patients, health professionals, firefighters and other stakeholders in Iran, who have practical experience or theoretical knowledge about management and action after fires. Data were analyzed based on a grounded theory approach, using the Paradigm model.
Results

Synergistic building safety deficiency and qualitative relief defects were identified as the center of this study. This reflects participants' experience essence on the barriers to preventing fires related injuries in residential areas in Iran. These issues are debatable at various levels, from local and national policy to individual characteristics and behaviors of people and inhabitants of residential buildings against fires.
Conclusions

The safety of building’s occupants requires the improvement of many factors at the local and national level. Safety construction of buildings, urban infrastructure and public education should be taken into consideration to promote community safety.
Luteolin Inhibits Respiratory Syncytial Virus Replication by Regulating the MiR-155/SOCS1/STAT1 Signaling Pathway
Authors: Wang Saisai , Ling Yiting , Yao Yuanyuan , Zheng Gang , Chen Wenbin
Published: June 2020

Background
Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infection in infants, children, immunocompromised adults, and elderly individuals. Currently, there are few therapeutic options available to prevent RSV infection. The present study aimed to investigate the effects of luteolin on RSV replication and the related mechanisms.
Material and methods
We pretreated cells and mice with luteolin before infection with RSV, the virus titer, expressions of interferon (IFN)-stimulated genes (ISGs), RSV-F and production of IFN-α and IFN-β were determined by plaque assay, RT-qPCR and ELISA. The activation of Janus kinase (JAK)- signal transducer and activator of transcription 1 (STAT1) signaling pathway was detected by Western blotting and luciferase assay. Proteins which negatively regulates STAT1 was determined by Western blotting. Then cells were transfected with suppressor of cytokine signaling 1 (SOCS1) plasmid and virus replication and ISGs expression was determined. Luciferase reporter assay and Western blotting was performed to detect the relationship between SOCS1 and miR-155.
Results
Luteolin inhibited RSV replication, as shown by the decreased viral titer and RSV-F mRNA expression both in vitro and in vivo. The antiviral activity of luteolin was attributed to the enhanced phosphorylation of STAT1, resulting in the increased production of ISGs. Further study showed that SOCS1 was downregulated by luteolin and SOCS1 is a direct target of microRNA-155 (miR-155). Inhibition of miR-155 rescued luteolin-mediated SOCS1 downregulation, whereas upregulation of miR-155 enhanced the inhibitory effect of luteolin.
Conclusion
Luteolin inhibits RSV replication by regulating the miR-155/SOCS1/STAT1 signaling pathway.
Advancing methodology for scoping reviews: recommendations arising from a scoping literature review (SLR) to inform transformation of children and adolescent mental health services.
Authors: Anderson Joanna K , Howarth Emma , Vainre Maris , Humphrey Ayla , Jones Peter B. , Ford Tamsin J.
Published: June 2020

Background: There is consensus that health services commissioning and clinical practice should be driven by scientific evidence. However, workload pressures, accessibility of peer reviewed publications and skills to find, appraise, and synthesise relevant evidence are often cited as barriers to uptake of research evidence by practitioners and commissioners alike. In recent years a growing requirement for rapid evidence synthesis to inform commissioning decisions about healthcare service delivery and provision of care contributed to an increasing popularity of scoping literature reviews (SLRs). Yet, published guidelines for conducting and reporting SLRs are still relatively scarce. Methods: The exemplar review used as a worked example aimed to provide a readily available, comprehensive, and user-friendly repository of research evidence for local commissioners to help them make evidence-informed decisions about redesigning East of England Children and Adolescent Mental Health Services. In conducting the review, we were broadly guided by Arksey and O’Malley’s framework, however some modifications were made at different stages to better reflect the largely pragmatic objective of this review. This paper compares the methodology used with existing methodological frameworks for scoping studies, to add to the existing knowledge base.Results: We proposed the following advancements to the existing SLR frameworks: (i) Assemble a research team with complementary skills and expertise; (ii); Draw on expertise of external partners, particularly practitioners, decision-makers and commissioners who will be translating findings into practice; (iii) Pre-register the review protocol. Keep a detailed record of all steps and decisions and consider how they would impact on generalisability and utility of review findings; (iv) Use systematic procedures for literature searchers, selection of studies, data extraction and analysis; (v) If feasible, appraise the quality of included evidence; (vi) Be transparent about limitations of findings.Conclusions: Despite some methodological limitations, scoping literature reviews are a useful method of rapidly synthesising a large body of evidence to inform commissioning and transformation of CAMHS. SLRs allow researchers to start with a broader questions, to explore the issue from different perspectives and perhaps find more comprehensive solutions that are not only effective, but also accounted for their feasibility and acceptability to key stakeholders.
miR-132-3p mediates Functions of Vascular Smooth Muscle Cells through targeting PTEN expression and ERK1/2 pathway
Authors: liu Guang , He zhijun , zhang Rong , Yang ganglion , Huang sheng , Lu huaxiang , lu xinwu , luo yanli , liu xiaobing
Published: June 2020

Background

This study was aimed to investigate the functional role of the microRNA (miR)-132-3p in rat vascular smooth muscle cells (VSMCs) and the potential mechanisms in abdominal aortic aneurysm (AAA).
Methods

VSMCs were transfected with miR-132-3p mimics and inhibitors, and the effects of miR-132-3p on VSMCs proliferation, migration were assessed by CCK-8 assay and Boyden chamber cell invasion assay, respectively. miRNA targets were determined using bioinformatics and luciferase reporter assays. The protein expression of phenotype markers and related signaling pathways were detected by Western blot.
Results

Overexpression of miR-132-3p in VSMCs attenuated VSMCs proliferation and migration. Conversely, the opposite effect was obtained with the inhibition of miR-132-3p. We further demonstrated that miR-132 could significantly promote the expression of VSMCs marker genes ACTA2 and MYH1. Reporter assays and western blot validated that PTEN as a direct target of miR-132-3p in VSMCs. Besides, miR-132-3p overexpression could also promote the expression of ras and c-myc, and activate the phosphorylation levels of ERK1/2.
Conclusions

These results indicate that miR-132-3p is a critical regulator in maintaining normal functions of VSMCs through PTEN-ERK1/2 axis. Restoring expression of miR-132-3p may serve as a potential therapeutic approach for treatment of AAAs.
Single and multiple suicide attempts: Prevalence and correlates in school-going adolescents in Liberia in 2017
Authors: Pengpid Supa , Peltzer Karl
Published: June 2020

Background: This investigation aimed to estimate the prevalence and its correlates of single and multiple suicide attempts among adolescents in Liberia.Method: Cross-sectional nationally representative data were analysed from 2,744 adolescents (18 years median age) that participated in the “2017 Liberia Global School-Based Student Health Survey (GSHS).”Results: Results indicate that 33.7% of students had made a suicide attempt in the past 12 months (16.5% single and 17.2% multiple suicide attempts). In adjusted multinomial logistic regression analysis, having no close friends, loneliness, having been frequently physically attacked, ever used amphetamine and fast food intake were associated with multiple suicide attempts in the past 12 months. In addition, having been frequently in a physical fight and current tobacco use were associated with single suicide attempt. In sex stratified analyses, in addition, among boys frequent bullying victimization and among girls, parental tobacco use and parents never or rarely check on home work were associated with multiple suicide attempts. Multiple psychosocial distressors, multiple social-environmental factors and multiple health risk behaviours were associated with single and multiple suicide attempts.Conclusion: One in three students had made suicide attempt in the past 12 months (one in six students multiple suicide attempts) and several associated variables were detected which can aid in designing intervention strategies.
Prognosis of lower respiratory tract infected patients with virus detected in bronchial alveolar lavage fluid: a retrospective observational study
Authors: Zhang Yuan , Huang Qiuping , Zhou Zhigang , Xie Yun , Li Xianchen , Jin Wei , Wang Ruilan
Published: June 2020

Background and Objectives The influence of virus detected in BALF is still debating. This study aimed to compare the prognosis of lower respiratory tract infected patients with virus detected in bronchial alveolar lavage fluid (BALF) and patients with virus undetected by using metagenomic next-generation sequencing technology.Methods This was a retrospective cohort study. 53 patients with lower respiratory tract infection were enrolled. BALF samples were collected from each patient and sent to perform mNGS pathogenic test in the study. According to the results of mNGS test, patients were divided into virus-detected group and virus-undetected group. In the meanwhile, patients’ clinical information, medical history, disease severity scores, parameters of organ function at the day of ICU admission, prognosis, hospital length of stay, ICU length of stay and needs for medical support were also collected.Results 39.6 percent (21/53) of the BALF samples were virus nucleic acid positive. Mortality rate, tracheotomy rate, mechanical ventilation supporting time, blood transfusion rate were significantly higher in virus-detected group than that in virus-undetected group. Virus-detected was closely related to hospital and ICU survival time.Conclusions Patients with virus detected in BALF were prone to a poorer prognosis. The detection of virus was a high-risk factor of death for LRTI patients. Virus-detected patients required more medical resources.
Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates
Authors: Chen Chun-mei , Wang Min , Li Xian-ping , Li Peng-ling , Tian Jing-jing , Zhang Kan , Luo Can
Published: June 2020

Background: Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections. It has been reported in many studies that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. But the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn’t been characterized yet.Results: 43 isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. Conclusions: These data showed that there might be a close homologous relationship between EXKP and EKP in neonates，indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.
Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort
Authors: Chen Yulian , Qiu Xingdi , Wang Wenjing , Li Dong , Wu Anyue , Hong Zubei , Di Wen , Qiu Lihua
Published: June 2020

Background: In this study, the association between human papillomavirus (HPV) infection and related cervical intraepithelial neoplasia (CIN) or cervical cancer and vaginal microbiome was evaluated in Chinese cohorts. Methods: The vaginal bacterial composition of five groups, HPV-infected women without CINs (HPV, n=78), women with low-grade squamous intraepithelial lesions (LSIL, n=51), women with high-grade squamous intraepithelial lesions (HSIL, n=23), women with invasive cervical cancer (Cancer, n=9) and healthy women without HPV infection (Normal, n=68), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Results: HPV infection increased vaginal bacterial richness and diversity regardless of the status of CINs. The vaginal bacterial richness and diversity were further augmented in women with cervical cancer. Lactobacillus was the most abundant genus in all groups. HPV infection had a negative influence on the abundances of Lactobacillus, Gardnerella and Atopobium. Accordingly, HPV infection increased the relative abundance of Prevotella, Bacillus, Anaerococcus, Sneathia, Megasphaera, Streptococcus and Anaerococcus. The increased proportions of Bacillus, Anaerococcus and the reduced abundance of Gradnerella vaginalis were probably related with the progression of CINs severity. HPV infection without CINs or cancerous lesions was strongly associated with Megasphaera. The most abundant bacterium in the LSIL group was Prevotella amnii. However, Prevotella timonensis, Shuttleworthia and Streptococcaceae at the family level were three taxa related to HSIL. Furthermore, more taxa were associated with the Cancer group including Bacillus, Sneathia, Acidovorax, Oceanobacillus profundus, Fusobacterium, Veillonellaceae at the family level, Anaerococcus and Porphyromonas uenonis. Samples in the Normal group were mostly assigned to CST III. HPV infection converted the vaginal bacterial community structure from CST III to CST IV. Furthermore, the proportions of CST IV were gradually augmented with the progression of the severity of CINs.Conclusions: This work interpreted the differential vaginal bacteria under HPV infection and various precancerous or cancerous lesions in a Chinese cohort. We distinguished the specific microbes and the vaginal bacterial structure that were related with the progression of CINs severity in Chinese women.
Screening household members of shepherds in brucellosis endemic areas in China and reporting follow-up results of asymptomatic infections
Authors: Hu Jing , Yang Huixin , Zhang Xiangyi , Zhang Siwen , Wang Taijun , Luo Jingjing , Hu Fangfang , Wang Dali , Ni Shaonan , Sheng Jian , Zhang Cui , Zhen Qing
Published: June 2020

Background: Brucellosis is a zoonotic infectious disease caused by brucella, patients often show obvious clinical manifestation, however, many cases of asymptomatic brucella infection were reported. Previous scholars have described or screened the asymptomatic infection, but little attention has been paid to the results. This research focused on the short-term results in patients with asymptomatic brucella infectionMethods: 595 household members of shepherds in brucellosis endemic areas were included, all of them have questionnaires and laboratory tests. Based on inclusion and exclusion criteria for the cohort, 15 asymptomatic infections were included and followed-up for 18months.Results: Among 595 subjects, 34(5.7%) were asymptomatic infections, 460(77.3%) were healthy, 58patients (9.7%) were diagnosed as brucellosis, 13(2.2%) suspected cases, 19(3.2%) cured cases and 11(1.8%) unclear diagnosis. Among 15 asymptomatic infections, the median age was 34 [12, 50] years, there were 40%cases <18 years old and the male-female ratio was 1.5:1, 60% cases were farmers and herdsmen, 11(73.3%) cases had a history of possible exposure to brucella. Average follow-up time was 10.47 ± 8.47 months. A total of 7 asymptomatic infections developed into brucellosis, of which five patients turned in the first month of follow-up, one patient in the second month, and one minor case turned in the seventh month. Remaining asymptomatic infections showed negative outcomes after 7 months of follow-up, among them, SAT titer decreased in two cases, no changes in SAT titer and clinical manifestations in six cases.Conclusions: Continued exposure to brucella may be a major risk factor for asymptomatic infection turn to brucellosis.
Effect of structured training programme on knowledge of stakeholders working for Particularly Vulnerable Tribal Groups (PVTGs) in twelve tribal districts of an eastern state of India
Authors: Bhatia Vikas , Giri Prajna Paramita , Taywade Manish
Published: June 2020

Background

Particularly Vulnerable Tribal Groups (PVTGs) are the most marginalized tribal communities in the Indian society. The uptake of government led health & nutrition programme by them is low due to the geographical remoteness of their habitat, communication gap between service providers and PVTGs and their cultural believe. The objective of this study is to implement & assess the effectiveness of training programmes for stakeholders working for PVTGs in tribal districts of Odisha, an eastern state of India.
Methods

A cross-sectional study was done in 12 tribal districts during April 2019 to January 2020. Six modules were developed for the trainings of different stakeholders like health officials, officials from Women & Child Development departments, NGO personnel, functionaries of Odisha Livelihood Mission (OLM) and Odisha PVTG Empowerment & Livelihood Programme (OPELIP). Three-day residential training programmes covered six thematic areas like child malnutrition, adolescent health, maternal health, communication in service delivery and about different PVTG communities and evaluation was done for 231 participants out of total 242 trained. Pretest and posttest knowledge assessment of the participants were done using a pretested structured questionnaire consisting of 20 items.
Results

The mean total score of knowledge was significantly improved after training as compared to before training among all stakeholders (13.79 ± 3.78 vs 11.5 ± 5.2, p value = 0.001). The pre-test result for the overall knowledge was low among OPELIP staff and health functionaries with mean score of 8.41 ± 3.03&9.16 ± 2.49 respectively. The post-test scores of health functionaries were highest in all domains and the overall increase in knowledge was statistically significant (pre-test 9.16 ± 2.49 vs 15.48 ± 4.19, p < 0.0001).
Conclusion

The assessment of pre & post test results revealed a significant improvement in knowledge of stakeholders. Hence, there should be administrative mechanism in government system to impart training to all stakeholders at regular intervals.
Implications of power imbalance in antenatal care-seeking among pregnant adolescents in rural Tanzania: a qualitative study
Authors: Mweteni Wemaeli , Kabirigi Julieth , Matovelo Dismas , Lassier Rose , Yohani Victoria , Shabani Girles , Shayo Prosper , Brenner Jennifer L. , Chaput Kathleen H.
Published: June 2020

Background: Adolescent girls (age 10-19 years) are at increased risk of morbidity and mortality due to pregnancy and childbirth complications, compared with older mothers. Low and middle-income countries, including Tanzania, bear the largest proportion of adolescent perinatal deaths globally. Most adolescent girls in Tanzania do not access antenatal care at health facilities, but the reasons for lack of antenatal care attendance are poorly understood. Methods: We conducted a qualitative thematic analysis study of the experiences of pregnant adolescents with accessing antenatal care in Misungwi district, Mwanza Region, Tanzania. We recruited 22 adolescent girls who were pregnant or parenting a child aged less than 5 years, using purposive sampling, and collected data about their lived experiences using in-depth individual interviews (IDIs). IDI data were triangulated with data from eight focus group discussions (FGDs) involving young fathers and elder men/women, and nine key informant interviews (KIIs) conducted with local health care providers. FGDs, KIIs and all but two IDIs were conducted and audiotaped in Swahili. All Swahili recordings were transcribed verbatim in Swahili. Two IDIs were conducted in local vernacular (Sukuma), and were transcribed into Swahili (as Sukuma is uncommon), by bilingual research assistants. All Swahili transcripts were then translated to English. A team of researchers analysed transcripts using emergent thematic analysis and constant comparison technique. Results: We identified four main themes: 1) Lack of maternal personal autonomy (Diminished power for decision making, Lack of financial and personal independence), 2) Stigma and judgment, 3) Vulnerability to violence and abuse, and 4) Knowledge about antenatal care. Conclusion: Pregnant adolescent care seeking for antenatal services is compromised by a complex power imbalance that involves financial dependence, lack of choice, lack of personal autonomy in decision making, experiences of social stigma, judgement, violence and abuse. Multi-level interventions are needed to empower adolescent girls, and to address policies and social constructs that may contribute to observed power imbalance; addressing these barriers can improve access to antenatal care among pregnant adolescents, and potentially reduce maternal morbidity and mortality.
Clinical and biochemical indexes of 11 COVID-19 patients and the genome sequence analysis of the tested SARS-CoV-2
Authors: Yu Zhikang , Wu Heming , Huang Qingyan , Guo Xuemin , Zhong Zhixiong
Published: June 2020

Background: At present, SARS-CoV-2 epidemic in the world rapidly spread. It is a serious global public health emergency.Methods: Here we described the clinical characteristics of 11 SARS-CoV-2 infected patients hospitalized in the Meizhou People's Hospital. And viral genome sequences of SARS-CoV-2 from these patients were analyzed.Results: Of the 11 patients, 6 cases developed fever, 9 cases developed cough, and 2 cases developed headache and chills. 4 patients (36.4%) had underlying diseases. Pneumonia is the most common complication. The most common laboratory abnormalities were decreased lymphocytopenia (LYM) and lymphocytic percentage (LYM%), decreased total protein (TP) and albumin (ALB), increased erythrocyte sedimentation rate (ESR), C reactive protein (CRP), activated partial thromboplastin time (APTT), increased fibrinogen (FIB), creatine kinase isoenzymes (CK-MB), and lactate dehydrogenase (LDH). Neutrophil (NEU) (r=0.664, P=0.026), CK-MB (r=0.655, P=0.029), blood urea nitrogen (BUN) (r=0.682, P=0.021) and SARS-CoV-2 virus cycle threshold (Ct) value were significantly correlated. Multiple sequence alignment (MSA) shows that we identified two different SNPs at positions 8781 and 28144.Conclusions: We hope that the reports of these 11 cases in our hospital will provide useful information for the diagnosis, treatment and drug development of SARS-CoV-2.
SOX9 in prostate cancer is upregulated by cancer-associated fibroblasts to promote tumor progression through HGF/c-Met-FRA1 signaling
Authors: Qin Haixiang , Yang Yang , Jiang Bo , Pan Chun , Chen Wei , Diao Wenli , Ding Meng , Cao Wenmin , Zhang Zhenxing , Ma Haoxing , Chen Mengxia , Gao Jie , Zhao Xiaozhi , Qiu Xuefeng , Guo Hongqian
Published: June 2020

Background
Previous studies have demonstrated that transcription factor SOX9 which was reactivated in prostate cancer (Pca) and promoted tumor growth was a poor prognostic biomarker for Pca. Nevertheless, the regulatory mechanism underlying SOX9 upregulation in Pca still remains unclear. Several cytokines widely distributed in the tumor microenvironment (TME) have been reported to be involved in the regulation of SOX9, suggesting that cancer-associated fibroblasts (CAFs), one of the main sources of secreted factors in TME, may play a role in regulating SOX9 expression.
Methods
Herein, an in vitro model of paracrine interaction between primary CAFs and Pca cells (both AR-positive and AR-negative Pca cells), was applied to investigate the molecular mechanism of SOX9 upregulation during Pca progression. The regulatory axis was validated by in vivo experiments and The Cancer Genome Atlas (TCGA) data.
Results
Conditional medium from Pca CAFs (CAF-CM) upregulated the expression of SOX9, which was also proved to be essential for CAF-induced tumor progression. Further analysis showed that it was hepatocyte growth factor (HGF) secreted by CAFs that was responsible for the SOX9 elevation in Pca cells via activating c-Met signaling. Mechanistically, HGF/c-Met signaling specifically activated MEK1/2-ERK1/2 pathway which then induced phosphorylated status and protein upregulation of FRA1. Furthermore, ChIP assay demonstrated that FRA1 transcriptionally upregulated SOX9 expression by binding to the TPA-responsive element (TRE) sequence in the promoter of SOX9 gene. We also found that HGF/c-Met-ERK1/2-FRA1-SOX9 axis was relatively conserved in human and mouse species by validating in mouse Pca cells (RM-1).
Conclusions
Our results revealed a novel insight into the molecular mechanism that SOX9 expression in Pca cells is promoted by CAFs, through the HGF/cMet-ERK1/2-FRA1 axis. Besides, SOX9 may serve as an alternative marker for the activated HGF/c-Met signaling to enroll the optimal Pca patients for HGF/c-Met inhibition treatment, since it is much more stable and easier to detect.
A New Perioperative Glucocorticosteroid Replacement Therapy for Cushing's Syndrome
Authors: Wang Liang , Yang Lin , Yang Yuanxing , Cui Zhe , Li Liming
Published: June 2020

Background: To evaluate the safety and effects of a new perioperative glucocorticosteroid replacement therapy for Cushing,s syndrome.Methods: A simple method of glucocorticosteroid replacement therapy as follow was adopted for 83 cases of Cushing's syndrome patient which were diagnosed before the operation: No glucocorticosteroid was used before operation, but during adrenal adenoma resection 100 mg hydrocortisone was given by intravenous injection，and another 200 mg hydrocortisone was given on the day of surgery. On day 1 and day 2 postoperative, 100 mg/12h and 100 mg/24h hydrocortisone were given by intravenous injection respectively. Prednisone(10mg/8h) was given from the day 2 postoperative by oral administration, then 5mg was reduced once a week until a maintenance dosage (5mg) was reached. Clinical symptoms were observed, plasma cortisol and 24h urine cortisol levels were intermittently measured post-operation to evaluate the safety and the curative effect of this new glucocorticosteroid replacement therapy.Results: Adrenal insufficiency and steroid withdrawal syndrome did not occur with all patients. Compared with the concentration of urine cortisol preoperation, it was significantly decreased on the day 7 postoperative. Plasma cortisol concentration was significantly decreased on day 6 postoperative compared with preoperative. All the patients were followed up to 6 months, plasma cortisol and 24h urine cortisol levels were all normal.Conclusions: This new glucocorticosteroid replacement therapy was safe, simple, and effective.
Interaction of Endogenous Proinflammatory Mediators on the Function of the Ex Vivo Perfused Human lung
Authors: Leligdowicz Aleksandra , Ross James T. , Nesseler Nicolas , Matthay Michael A.
Published: June 2020

Background The ex vivo human perfused lung model has enabled optimizing donor lungs for transplantation and delineating mechanisms of lung injury. Perfusate and airspace biomarkers are a proxy of the lung response to experimental conditions. However, there is a lack of studies evaluating biomarker kinetics during perfusion and after exposure to stimuli. In this study we analyzed the ex vivo perfused lung response to three key perturbations: exposure to the perfusion circuit, exogenous fresh whole blood, and bacteria. Results 99 lungs rejected for transplantation underwent ex vivo perfusion. One hour after reaching experimental conditions, fresh whole blood was added to the perfusate (n=55). Two hours after reaching target temperature, Streptococcus pneumoniae was added to the perfusate (n=42) or to the airspaces (n=17). Perfusate and airspace samples were collected at baseline (once lungs were equilibrated for 1 hour, but before blood or bacteria were added) and 4 hours later. Interleukin (IL)-6, IL-8, Angiopoietin (Ang)-2, and soluble tumor necrosis factor receptor (sTNFR)-1 were quantified. Baseline perfusate and airspace biomarker levels varied significantly, and this was not related to pre-procurement PaO2:FiO2 ratio, cold ischemia time, and baseline alveolar fluid clearance (AFC). After 4 hours of ex vivo perfusion, the lung demonstrated a sustained production of proinflammatory mediators. The change in biomarker levels was not influenced by baseline donor lung characteristics (cold ischemia time, baseline AFC) nor was it associated with measures of experimental epithelial (final AFC) or endothelial (percent weight gain) injury. In the presence of exogenous blood, the rise in biomarkers was attenuated. Lungs exposed to intravenous (IV) bacteria relative to control lungs demonstrated a significantly higher rise in perfusate IL-6. Conclusions The ex vivo perfused lung has a marked endogenous capacity to generate inflammatory responses over the course of short-term perfusion. The lack of association between biomarker change and donor lung cold ischemia time as well as final alveolar fluid clearance and experimental percent weight gain suggests that the maintained ability to produce biomarkers is not merely a marker of lung epithelial or endothelial injury but may support the lung’s role as an immune cell reservoir.
Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway by TLR2/4 activation
Authors: Wu Meng-Huang , Lin Chuang-Yu , Hou Chun-Yin , Sheu Ming-Thau , Chang Hsi
Published: June 2020

Background
Ganoderma sp., such as Ganoderma tsugae (GT), play an important role in traditional Chinese medicine. Ganoderma sp. contains several constituents, including Sacacchin, which has recently drawn attention because it can not only enhance the repair of muscle damage but also strengthen the muscle enforcement. Although Ganoderma sp. have a therapeutic effect for neuromuscular disorders, the underlying mechanism remains unclear. This study investigated the effect and underlying molecular mechanism of micronized sacchachitin (mSC) on satellite cells (SCs), which are known as the muscle stem cells.
Methods
The myogenic cells, included SCs (Pax7+) were isolated from tibialis anterior muscles of a healthy rat and were cultured in growth media with different mSC concentrations. For the evaluation of SC proliferation, these cultivated cells were immunostained with Pax7 and bromodeoxyuridine assessed simultaneously. The molecular signal pathway was further investigated by using Western blotting and signal pathway inhibitors.
Results
Our results revealed that 200 µg/mL mSC had an optimal capability to significantly enhance the SC proliferation. Furthermore, this enhancement of SC proliferation was verified to be involved with activation of TAK1-JNK-AP-1 signaling pathway through TLR2, whose expression on SC surface was confirmed for the first time here.
Conclusion
Micronized sacchachitin extracted from GT was capable of promoting the proliferation of SC under a correct concentration.
‘I would have stayed home if I could manage it alone’; testimony of an Ethiopian mother abandoned by care providers during facility based childbirth: a case report
Authors: Adinew Yohannes , Hall Helen , Marshall Amy , Kelly Janet
Published: June 2020

Background: Every woman is entitled to respectful care during pregnancy and childbirth as a basic human right. However, not all women are being treated equally well. Case presentation: This case study highlights some of the common disrespectful practices that women face. This is a testimony of a 28-year-old mother of two, narrated in her own words. The data were collected during an in-depth interview in November 2019. The interview was conducted in her house and her name has been changed to protect her identity. The interview was audio-taped by a digital voice recorder, later transcribed and translated verbatim from the local language, Amharic, to English.Conclusion: This woman’s story highlights the unfortunate reality for some women. Five themes emerged from her narrative: denial of care, non-consented care, non-dignified care, taking a sick baby home with resentment, and loss of trust of care providers.
ESTRATÉGIAS DE GESTÃO DA QUALIDADE E GESTÃO DE MATERIAIS DE UMA FARMÁCIA HOSPITALAR: REVISÃO INTEGRATIVA DA LITERATURA
Authors: Torres Dantas da Silva Rafaela , Silva dos Santos Fernanda Julyanna , Dos Santos Bezerril Manacés , Salvador Pétala
Published: June 2020

Objetivo: identificar as estratégias de gestão da qualidade e gestão de materiais utilizadas para a organização do processo de trabalho da farmácia hospitalar. Método: trata-se de uma Revisão Integrativa da Literatura realizada em quatro bases de dados. A coleta de dados ocorreu no mês de setembro de 2019. Os dados foram analisados por meio de estatística descritiva, de modo que foi possível fazer a contabilização e a descrição dos dados encontrados.  Resultados: a amostra foi composta por 29 artigos, todos publicados no Brasil e a região que teve uma maior quantidade de artigos disponíveis foi a Sudeste. O instrumento de organização mais utilizado foi o questionário estruturado, citado em 17,24% (n=05) dos estudos, também foi identificado a utilização de ferramentas de gestão da qualidade. Dentre os benefícios, os mais citados foram a redução de custos para as instituições e a otimização do trabalho. Conclusão: a gestão do setor de farmácia hospitalar ainda é pouco discutida e a falta de trabalhos sobre o assunto se apresentou como uma dificuldade para o desenvolvimento da revisão de literatura.

Palavras-chave:  Gestão de materiais. Controle de qualidade. Serviço de farmácia hospitalar. Gestão em saúde.
ELEMEN ARSITEKTUR, INTERIOR DAN SIGNAGE UNTUK MENEMUKAN JALAN DAN BRANDING UNIVERSITAS KRISTEN PETRA
Authors: Tanuwidjaja Gunawan , Wijaya Nerissa Arviana , Widyanto Lavenia , Wiarta Stephanie Seaver , Sugianto John Kenley
Published: June 2020

Title: Architectural, Interior Elements and Signage for Wayfinding and Branding in Petra Christian University

Petra Christian University (PCU) is a leading private University in Surabaya. PCU possessed advantage in facility?s quality as well as educational service. This was clearly visible in the campus building design that played as architecture, interior and graphic wayfinding elements, especially in W Building (Radius Prawiro Building), EH Building (Entrance Hall) and T Building. Further these elements could also support the University?s Branding and were integrated in the PCU?s integrated wayfinding system.
PERAN PEREMPUAN DALAM MERUMAH
Authors: Koniherawati  , Pratikno Priyo
Published: June 2020

Title: The Roles of Wemen in Domestic Activities

Women, especially mother, has a major role in build and maintain a home, as shown by various ethnic groups in the world. The various roles of woman indicated in protection, give a sense of comfort, and safety for the baby and all family member. Mother is person who gives a shade of home through food processing activities and how to primp her self. The efforts collecting building materials, to decorate, and chose home furnishings are dominant activities that mother does. This study is explore the problems of designing of home by women in the past were intended to justify the design of art and architecture. This study based on literature that shows the role of the mother in build, manage, and interpreting a house. The method of this study base on secondary data and then weave every aspects to build the hypotheses. These information show that women as housewives have a significant role in shaping the house as culturally or physically. The results showed that women as mother very dominant in giving the feel and shape of house.
PENINGKATAN VISIBILITAS BANGUNAN LAMA, STUDI KASUS JALAN KREMBANGAN BARAT SURABAYA
Authors: Nugroho Setyo
Published: June 2020

Title: The Improvement of Old Building Visibility, Krembangan Barat Street of Surabaya as of Case Study

High development in the City center forces old building losing its existence. The main factor old buildings losing its existences are the lack of skyline guideline (new buildings obstruct the view toward old buildings), the changes of traffic direction, and the selection of vegetation type. This paper discusses a brief evaluation of old building visibility through visual experiences, and gives a schematic design as proposal for jalan Krembangan Barat. Serial vision technique analysis is addressed in order to gain the visual and spatial experiences of the built environment. Result shows that three spots of place should be improved in order to perceive the visibility by rehabilitating, adaptive re-using, and providing pedestrian ways to connect one potential spot to others in the corridor of Krembangan Barat.
KINERJA TERMAL RUMAH NIANG DI DATARAN TINGGI TROPIS LEMBAB DI DISTRIK MANGGARAI
Authors: Alfred D.D. P. Jhon , Antaryama I Gusti Ngurah , N.E. Sri Nastiti
Published: June 2020

Title: The Performance of Niang House in the Tropical Highland of Manggarai District

Niang home is one of the ten varieties of Traditional Architecture in East Nusa Tenggara Province located in the highlands of Manggarai district (800 - 1200 meters above sea level) with its peculiarities, namely the circular floor plan and a conical roof. Niang home is growth by people with all kinds of traditions and utilize a variety of local potentials such as materials, technology and knowledge. But local potentials such as the use of lightweight materials such problems occur in upland areas that tend to be cold because the thermal properties of lightweight material is loose and flowing out so that it can potentially create underheating and conditions affecting comfort conditions in buildings. This study aimed to evaluate the effect of house design of Niang in response to thermal conditions in the highlands and analyze the behaviour of the material of the design elements that affect the thermal performance of the house of Niang. The study was conducted by the method of observation and simulation with the help of software ARCHIPAK version 5.0. The final results of this study indicate that the design of Niang house can not provide adequate thermal comfort, especially at night until morning, where the condition of the buildings tend to underheating in considerable period of time. From the simulation can also be seen that the floor and the roof is the most critical element of the heat release.