bluuebunny/crossref_metadata_2025 huggingface dataset:
An exploratory study of drawings as a tool to evaluate student understanding of the Food-Energy-Water (FEW) Nexus
Authors: Horne Lydia , Manzanares Amanda , Atalan-Helicke Nurcan , Vincent Shirley , Anderson Steven W. , Romulo Chelsie L.
Published: May 2024

As future decision-makers, students must develop interdisciplinary, systems thinking skills to make effective management decisions; however, systems thinking remains challenging for many students. Here, we use the Food-Energy-Water (FEW) Nexus as a framework to examine how drawings can help students cultivate systems thinking skills. Drawings can be tools to make implicit mental models of systems connections explicit for instructors to better comprehend student learning. Our goal was to understand how drawing can help students make connections across systems compared to using only verbal explanations. In 2021, we interviewed undergraduates, asking them to draw and verbally explain the FEW Nexus. Analysis revealed that student drawings showed an increase in the number of connections that half of students could describe when compared to verbal-only explanations. Instructors may benefit from this study by recognizing areas where students might struggle to understand FEW Nexus connections, where additional course emphasis is needed, and how drawings can help assess student learning.
Comparison of fatigue crack growth design curves on GMAW and EBW joints of high strength steels
Authors: Sisodia R. P. S. , Gáspár M. , Lukács J.
Published: May 2024

There is a growing demand in the industrial sector for the use of high-strength structural steels (HSSSs), which can achieve a significant weight reduction in structures. These structural steels are usually produced by quenching and tempering (Q + T) or thermomechanical treatment (TM), and their applications in welded structures pose several challenges for the users. In industrial practice, gas metal arc welding (GMAW) is basically the most commonly used fusion welding process, which has a relatively high heat input. However, at HSSSs, there is a need for low heat input but, at the same time, productive welding processes. High-energy density welding processes, e.g., electron beam welding (EBW), offer a unique opportunity to weld these steels. The widespread use of HSSSs is also hampered by the fact that the benefits of high strength can be exploited primarily under static loading. At the same time, different welded structures made of HSSSs are often subjected to cyclic loading, and possible weld defects and material discontinuities are major risks in this case. During our experiments, GMAW and autogenous EBW processes were applied to make welded joints from S960 Q + T and TM structural steels. The fatigue resistance of the welded joints was characterized by fatigue crack growth (FCG) tests, considering the increased crack sensitivity of HSSSs. A statistical approach was followed both in the design of the experiments and in the evaluation of their results. Based on the test results fatigue crack propagation design curves were determined for the investigated GMAW and EBW welded joints. The design curves were compared with each other, with design curves of lower strength material (S690QL) and with the recommended fatigue crack growth laws of BS 7910.
Mesoporous Pdx-Nix aerogels for electrocatalytic evaluation of urea-assisted electrolysis
Authors: Rodríguez-Buenrostro A. , Martínez-Lázaro A. , Contreras-Martínez M. V. , Sharma Ashutosh , Barcenas G. Luna , oza Goldie , Arenillas A. , Ledesma-García J. , Arriaga L. G.
Published: May 2024

This work presents the synthesis and evaluation of Pd-Ni aerogels toward the urea oxidation reaction (UOR). The incorporation of Ni led to a 0.13 V reduction in the energy required for the oxidation and reduction of PdO compared to monometallic Pd, both in alkaline medium with and without urea. Varying the Ni ratios in Pd (Pd-Ni 4:1, Pd-Ni 1:1, and Pd-Ni 1:4) led to significant changes in the electrochemical behaviour. In alkaline medium without urea, PdNi 4:1 showed the formation of NiOOH at 1.35 V, which promoted oxygen diffusion on the electrode surface and increased the current density, confirming the increase in the active sites of NiOOH and NiPdOOH and enabling urea-based electrolysis at these sites. While palladium aerogels alone are ineffective for UOR, the presence of nickel plays a key role in enhancing the UOR efficiency. On the other hand, physicochemical characterisation revealed that PdNi 4:1 has a crystal size of 4.37 nm and a larger shift in the 2θ positions of the (111) and (200) planes, which favours electronic changes that were investigated by XPS. These changes affected the electrocatalytic activity, which is primarily related to electronic effects. The results of SEM and TEM studies and nitrogen adsorption-desorption isotherm confirmed that the aerogels are highly porous and have an effective surface area and abundant active sites for reactions that allow efficient mass transfer and low diffusion resistance. TEM observations revealed interconnected nanochains indicating optimal electrocatalytic activity for both ORR and UOR due to high mass transfer. These interconnected networks are crucial for improving electrocatalytic activity in the urea oxidation reaction.
Prediction model to identify infectious COVID-19 patients in the emergency department
Authors: Aung Myat Oo , Venkatachalam Indumathi , Sim Jean X.Y. , Wee Liang En , Aung May K. , Yang Yong , Conceicao Edwin P. , Arora Shalvi , Lee Marcus A.B. , Sia Chang H. , Tan Kenneth B.K. , Ling Moi Lin
Published: May 2024

Background:

Real-time reverse-transcriptase polymerase chain reaction (RT-PCR) has been the gold standard for diagnosing coronavirus disease 2019 (COVID-19) but has a lag time for the results. An effective prediction algorithm for infectious COVID-19, utilized at the emergency department (ED), may reduce the risk of healthcare-associated COVID-19.

Objective:

To develop a prototypic prediction model for infectious COVID-19 at the time of presentation to the ED.

Material and methods:

Retrospective cohort study of all adult patients admitted to Singapore General Hospital (SGH) through ED between March 15, 2020, and December 31, 2022, with admission of COVID-19 RT-PCR results. Two prediction models were developed and evaluated using area under the curve (AUC) of receiver operating characteristics (ROC) to identify infectious COVID-19 patients (cycle threshold (Ct) of <25).

Results:

Total of 78,687 patients were admitted to SGH through ED during study period. 6,132 of them tested severe acute respiratory coronavirus 2 positive on RT-PCR. Nearly 70% (4,226 of 6,132) of the patients had infectious COVID-19 (Ct<25). Model that included demographics, clinical history, symptom and laboratory variables had AUROC of 0.85 with sensitivity and specificity of 80.0% & 72.1% respectively. When antigen rapid test results at ED were available and added to the model for a subset of the study population, AUROC reached 0.97 with sensitivity and specificity of 95.0% and 92.8% respectively. Both models maintained respective sensitivity and specificity results when applied to validation data.

Conclusion:

Clinical predictive models based on available information at ED can be utilized for identification of infectious COVID-19 patients and may enhance infection prevention efforts.
The Dynamics of Herder-Farmer Conflicts in Plateau State, Nigeria, and Central Darfur State, Sudan
Authors: Babatunde Abosede Omowumi , Ibnouf Fatma Osman
Published: May 2024

Drawing on their own field studies, the authors examine how state and local actors involved in resource management and peacebuilding activities are implicated in the conflict between farmers and herders in Plateau State, Nigeria, and Central Darfur State, Sudan. The authors show that state officials, traditional chiefs, and security agents intensified the conflict by perpetuating the inequitable distribution of resources needed for the survival of farmers and herders, while promoting a peacebuilding process that empowered some groups and disempowered others. The divisive role state and local actors played accentuated the socio-political grievances underlying the conflict and enervated the peacebuilding process.
Self-Determination and the Value of Nationality
Authors: Maguire Ruairi
Published: May 2024

In this article, I argue that because co-nationals have an intrinsically valuable relationship, they have a presumptive claim against interference in their collective affairs. My argument from the claim that co-nationals have an intrinsically valuable relationship to the presumptive claim against interference is threefold, and I set it out in section “From Intrinsic Value to Self-Determination”: firstly, parties to an intrinsically valuable relationship have a respect-based claim to autonomy. Secondly, the relationship between co-nationals realizes some important goods, and collective autonomy is internally related to these. Finally, the fact that co-nationals have an intrinsically valuable relationship, and affective attachments means that they have a strong interest in carrying out certain activities together, without interference from outsiders. In section “Grounding the Presumptive Claim,” I argue that these three grounds cumulatively amount to a presumptive claim to collective autonomy. I outline the implications for the issue of secession.
The African Guiding Principles on the Rights of Migrants, Refugees and Asylum Seekers (Afr. Comm'n H.P.R.)
Authors: Dirar Luwam , Kysel Ian M. , Raach Fatma
Published: May 2024

In its 75th ordinary session, the African Commission on Human and Peoples’ Rights (ACHPR, or Commission), the human rights organ of the African Union, with a mandate to promote and protect human and peoples’ rights and interpret the African Charter on Human and Peoples’ Rights, unanimously adopted the African Guiding Principles on the Human Rights of Migrants, Refugees and Asylum Seekers (Guiding Principles). They were publicly launched by the Commission at its 77th ordinary session on October 21, 2023. The drafting was led by the Commission's Special Rapporteur on Refugees, Asylum Seekers, Internally Displaced Persons, and Migrants in Africa. The thirty-eight Guiding Principles are based largely on African regional treaty law, case law, standards, and resolutions of the Commission and also draw from complementary international law. In addition, the Guiding Principles draw from the experience of other world regions, including adoption by the Inter-American Commission on Human Rights (the human rights organ of the Organization for American States) of the 2019 Inter-American Principles on the Human Rights of All Migrants, Refugees, Stateless Persons, and Victims of Human Trafficking.
A survey of context in neural machine translation and its evaluation
Authors: Castilho Sheila , Knowles Rebecca
Published: May 2024

The question of context in neural machine translation often focuses on topics related to document-level translation or intersentential context. However, there is a wide range of other aspects that can be considered under the umbrella of context. In this work, we survey ways that researchers have incorporated context into neural machine translation systems and the evaluation thereof. This includes building translation systems that operate at the paragraph level or the document level or ones that translate at the sentence level but incorporate information from other sentences. We also consider how issues like terminology consistency, anaphora, and world knowledge or external information can be considered as types of context relevant to the task of machine translation and its evaluation. Closely tied to these topics is the question of how to best evaluate machine translation output in a way that is sensitive to the contexts in which it appears. To this end, we discuss work on incorporating context into both human and automatic evaluations of machine translation quality. Furthermore, we also discuss recent experiments in the field as they relate to the use of large language models in translation and evaluation. We conclude with a view of the future of machine translation, where we expect to see issues of context continue to come to the forefront.
Nationalism, Status, and Conspiracy Theories: Evidence from Pakistan
Authors: Mir Asfandyar , Siddiqui Niloufer
Published: May 2024

Does nationalism increase beliefs in conspiracy theories that frame minorities as subversives? From China to Russia to India, analysts and public commentators increasingly assume that nationalism fuels belief in false or unverified information. Yet existing scholarly work has neither theoretically nor empirically examined this link. Using a survey experiment conducted among 2,373 individuals and 6 focus groups with 6–8 participants each, for a total of 50 individuals, we study the impact of nationalist sentiment on belief in conspiracy theories related to ethnic minority groups in Pakistan. We find that nationalist primes – even those intended to emphasize the integration of diverse groups into one superordinate national identity – increase belief in statements about domestic minorities collaborating with hostile foreign powers. Subgroup analysis and focus groups suggest that nationalism potentially increases the likelihood that one views rights-seeking minorities as undermining the pursuit of national status.
Effect of calcium soaps from garlic (Allium sativum) and willow (Salix babylonica) extracts on nematode loads, nutrient intake and digestibility, nitrogen balance and rumen fermentation kinetics in dairy goats
Authors: Vargas-Bello-Pérez Einar , Ghavipanje Navid , Torres-Gonzalez Teresa , Angeles-Hernandez Juan Carlos , Velázquez-Ordoñez Valente , Castelán-Ortega Octavio Alonso , Robles Jimenez Lizbeth E. , Roskopf Sergio Daniel , Gonzalez-Ronquillo Manuel
Published: May 2024

The objective of this study was to determine the effect of dietary calcium soaps from garlic (Allium sativum) and willow (Salix babylonica) extracts on nematode loads, nutrient intake and digestibility, nitrogen balance and rumen fermentation kinetics in dairy goats. Nine adult non-lactating Saanen goats were grouped into a complete randomized block design with 3 treatments (n = 3) over a period of 28 d. Animals were fed a diet based on alfalfa hay and a concentrate that was supplemented (65 g/kg DM) with calcium soaps of safflower (control), garlic or willow. Intake of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) were not affected by dietary calcium soaps. However, the highest digestibility of DM and OM were observed in willow supplemented goats. In vitro gas kinetics and fermentation profile were not affected by diets. Results from fecal egg count indicated a reduction in total count, Haemonchus spp. and Trychostrongylus spp. for both garlic and willow compared to control. Our results suggest that calcium soaps of garlic or willow extracts can be used to reduce gastrointestinal parasites in goats without compromising productive traits or rumen function.
Palaeo-environmental significance of evaporative calcite crusts in the Untersee Oasis, East Antarctica
Authors: Lacelle Denis , Christy Michelle , Faucher Benoit , Sobron Pablo , Andersen Dale
Published: May 2024

Secondary carbonate precipitates on the surface of clasts have rarely been reported from Antarctica. Here, we infer the origin, age and palaeo-environmental significance of the calcite crusts in the Untersee Oasis, East Antarctica. The distribution of calcite crusts, which are up to 1 mm thick, is limited to locations with residual snow patches, and they have some of the highest δ18O (up to +17.4‰ Vienna Standard Mean Ocean Water (VSMOW)) and δ13C (up to +14.6‰ Vienna Pee Dee Belemnite (VPDB)) compositions of any carbonate deposits in terrestrial polar environments. Their δ18O and δ13C values are substantially enriched with respect to the isotopic values expected from equilibrium precipitation from the δ18O and δ13CDIC (DIC = dissolved inorganic carbon) of snow meltwater. The formation of the calcite crusts is ascribed to the evaporation of residual snow meltwater and the low relative humidity and strong winds, favouring a kinetic isotope effect. The 14C age distribution of the calcite crusts (1550 cal yr bp to modern) provides a minimum age for ice retreat and drainage of the palaeo-lake in Aurkjosen Cirque. However, in this polar desert environment in which surface melting is limited, the calcite crusts require sufficient snow accumulation and air temperatures warm enough to generate meltwater, and their age distribution corresponds to the late Holocene warm-wet climate period.
Negotiating the representations of the village in socialist Czechoslovakia
Authors: Doušek Roman
Published: May 2024

This study deals with shared ideas about the countryside and its inhabitants during the Communist regime in Czechoslovakia. It identifies the most significant sources of contemporary representations of the rural. These include, besides historical ideas pre-dating the regime, the party-state itself, expert structures, the arts, and pop culture. In many cases, representations encompassed efforts to transform the countryside, or society, or to maintain the status quo, and, following de Certeau, we can consider such activities to be strategies. On the other hand, villagers approached these representations and strategies using their own tactics. This study demonstrates that rural policymaking was not just in the hands of power structures; the effects it had depended a great deal on the dominated rural inhabitants.
‘Blown from a gun’: situating the British practice of execution by cannon in the context of southern and western Asia
Authors: Jenzen-Jones N. R. , Randall Charles , Shanley Jack , Sayadi Omer
Published: May 2024

In parts of southern and western Asia, as elsewhere, the cannon once served as one of the most dramatic tools in the inventories of state executioners. The practice of ‘blowing from a gun’, by which the condemned was bound to the front of a cannon and quite literally blown to pieces, was most infamously employed in British India and the Princely States, and the vast majority of English-language scholarship focuses on these regions. However, blowing from guns was commonplace in several other contemporary states, and the British use of the practice has rarely been situated in this context. The tactic was considered especially useful in Persia and Afghanistan, where weak governance, rebellion, and rampant banditry all threatened the legitimacy of the nascent state in the nineteenth and early twentieth centuries. This article presents a history of the practice of execution by cannon in southern and western Asia, positioning it within the existing literature on public executions in the context of military and civilian justice. In doing so, the article seeks to situate the British use of the tactic within a broader regional practice, arguing that, whilst the British—following the Mughal tradition—used execution by cannon primarily in maintaining military discipline, states such as Persia and Afghanistan instead employed the practice largely in the civilian context. This article also provides a brief technical review of the practice, drawing upon numerous primary sources to examine execution by cannon within the Mughal empire, British India, Persia, and Afghanistan.
Princely prisons, state exhibitions, and Muslim industrial authority in colonial India
Authors: Lanzillo Amanda
Published: May 2024

This article analyses the prison industries and state industrial exhibitions of three Indian princely states in the late nineteenth and early twentieth centuries, tracing how princely elites sought to develop distinct labouring and industrial cultures. Drawing on examples from three Muslim-led princely states, namely Rampur, Bhopal, and Hyderabad, the article argues that state elites distinguished their forms of cultural and religious authority from that of the British Raj by coercing and displaying new industrial practices. They aimed to cultivate an industrial modernity that could compete with colonial projects while also promoting what they characterised as Indian Muslim characteristics and courtly traditions for artisan labourers and their work. The article asks how princely elites worked to conscript their subjects—including marginalised subjects such as convict labourers—into visions of regional industrial authority. Princely visions of Muslim and courtly industrial futures in Rampur, Bhopal, and Hyderabad were rooted in the attempts of state administrators to fashion distinctive regional identities and assert authority in a context of circumscribed, quasi-colonial rulership. Industrial cultures associated with princely prisons and exhibitions ultimately exceeded the bounds of these projects, placing pressure on other state subjects to adopt new material practices and engage with state-defined regional craft traditions.
Kerosene Is King: Kerosene Consumers and the Antitrust Movement against Standard Oil, 1859–1911
Authors: Jang Minseok
Published: May 2024

In the late nineteenth-century United States, kerosene became a universal illuminant for artificial lighting, providing its users with a shared material environment. While kerosene users employed the fluid not only for lighting but also for washing, cooking, and cleaning, they had to deal with the material’s risks, such as fires and explosions. With the help of chemists and domestic advisors, American consumers adapted to this ambivalent material condition, weaving kerosene into their economic life and social thought. In so doing, some consumers identified as a “professional class” that navigated within this material environment through their own expertise—which paralleled their economic struggle within a rapidly growing but volatile political economy during the Gilded Age. As Standard Oil’s monopolization of the kerosene business became a substantial issue in national politics, this social consciousness among kerosene users attracted anti-monopolists like Ida Tarbell. Because Standard Oil had lowered the consumer price, these reformers sought an alternative rationale to persuade kerosene-consuming households to participate in the antitrust movement against the company. Examining how these progressive reformers turned kerosene consumers’ social identity to their political ends, this article sheds new light on the relationship between the energy transition, consumer culture, and American capitalism.
CHORUS: A New Dataset of State Interest Group Policy Positions in the United States
Authors: Hall Galen , Basseches Joshua A. , Bromley-Trujillo Rebecca , Culhane Trevor
Published: May 2024

Research on the activities and influence of interest groups in state legislatures faces a data problem: we are missing a comprehensive, systematic dataset of interest groups’ policy preferences on state legislation. We address this gap by introducing the Dataset on Policy Choice and Organizational Representation in the United States (CHORUS). This dataset compiles over 13 million policy positions stated by tens of thousands of interest groups and individuals on bills in 17 state legislatures over the past 25 years. We describe the process used to construct CHORUS and present a new network science technique for analyzing policy position data from interest groups: the layered stochastic block model, which groups similar interest groups and bills together, respectively, based on patterns in the policy positions. Through two demonstrative applications, we show the utility of these data, combined with our novel analytical approach, for understanding interest group configurations in different state legislatures and policy areas.
Distant Stars Spotlight Mini Moons in Saturn’s Rings
Authors: Kornei Katherine
Published: May 2024

By studying how starlight attenuates as it travels through the icy particles encircling Saturn, researchers have zoomed in on a host of small structures in the gas giant’s famous rings.
Enrichment of a subset of Neanderthal polymorphisms in autistic probands and siblings
Authors: Pauly Rini , Johnson Layla , Feltus F. Alex , Casanova Emily L.
Published: May 2024

Homo sapiens and Neanderthals underwent hybridization during the Middle/Upper Paleolithic age, culminating in retention of small amounts of Neanderthal-derived DNA in the modern human genome. In the current study, we address the potential roles Neanderthal single nucleotide polymorphisms (SNP) may be playing in autism susceptibility in samples of black non-Hispanic, white Hispanic, and white non-Hispanic people using data from the Simons Foundation Powering Autism Research (SPARK), Genotype-Tissue Expression (GTEx), and 1000 Genomes (1000G) databases. We have discovered that rare variants are significantly enriched in autistic probands compared to race-matched controls. In addition, we have identified 25 rare and common SNPs that are significantly enriched in autism on different ethnic backgrounds, some of which show significant clinical associations. We have also identified other SNPs that share more specific genotype-phenotype correlations but which are not necessarily enriched in autism and yet may nevertheless play roles in comorbid phenotype expression (e.g., intellectual disability, epilepsy, and language regression). These results strongly suggest Neanderthal-derived DNA is playing a significant role in autism susceptibility across major populations in the United States.
The role of peripheral white blood cell counts in the association between central adiposity and glycemic status
Authors: Liu Fengqiong , Li Yanni , Li Wanxin , Feng Ruimei , Zhao Hongwei , Chen Jun , Du Shanshan , Ye Weimin
Published: May 2024

Aims

Although central adiposity is a well-known risk factor for diabetes, the underlying mechanism remains unclear. The aim of this study was to explore the potential mediation role of circulating WBC counts in the association between central adiposity and the risk of diabetes.

Materials and methods

A cross-sectional study was conducted using data from the Fuqing cohort study, which included 6,613 participants aged 35–75 years. Logistic regression analysis and Spearman’s rank correlation analysis were used to examine the relationships between waist-to-hip ratio, WBC counts and glycemic status. Both simple and parallel multiple mediation models were used to explore the potential mediation effects of WBCs on the association of waist-to-hip ratio with diabetes.

Results

The study revealed a positive relationship between waist-to-hip ratio and risk of prediabetes (OR = 1.53; 95% CI, 1.35 to 1.74) and diabetes (OR = 2.89; 95% CI, 2.45 to 3.40). Moreover, elevated peripheral WBC counts were associated with both central adiposity and worsening glycemic status (P < 0.05). The mediation analysis with single mediators demonstrated that there is a significant indirect effect of central adiposity on prediabetes risk through total WBC count, neutrophil count, lymphocyte count, and monocyte count; the proportions mediated were 9.92%, 6.98%, 6.07%, and 3.84%, respectively. Additionally, total WBC count, neutrophil count, lymphocyte count, monocyte count and basophil count mediated 11.79%, 11.51%, 6.29%, 4.78%, and 1.76%, respectively, of the association between central adiposity and diabetes. In the parallel multiple mediation model using all five types of WBC as mediators simultaneously, a significant indirect effect (OR = 1.09; 95% CI, 1.06 to 1.14) were observed, with a mediated proportion of 12.77%.

Conclusions

Central adiposity was independently associated with an elevated risk of diabetes in a Chinese adult population; levels of circulating WBC may contribute to its underlying mechanisms.
Monosymmetric Fe-N4 sites enabling durable proton exchange membrane fuel cell cathode by chemical vapor modification
Authors: Bai Jingsen , Zhao Tuo , Xu Mingjun , Mei Bingbao , Yang Liting , Shi Zhaoping , Zhu Siyuan , Wang Ying , Jiang Zheng , Zhao Jin , Ge Junjie , Xiao Meiling , Liu Changpeng , Xing Wei
Published: May 2024

The limited durability of metal-nitrogen-carbon electrocatalysts severely restricts their applicability for the oxygen reduction reaction in proton exchange membrane fuel cells. In this study, we employ the chemical vapor modification method to alter the configuration of active sites from FeN4 to the stable monosymmetric FeN2+N’2, along with enhancing the degree of graphitization in the carbon substrate. This improvement effectively addresses the challenges associated with Fe active center leaching caused by N-group protonation and free radicals attack due to the 2-electron oxygen reduction reaction. The electrocatalyst with neoteric active site exhibited excellent durability. During accelerated aging test, the electrocatalyst exhibited negligible decline in its half-wave potential even after undergoing 200,000 potential cycles. Furthermore, when subjected to operational conditions representative of fuel cell systems, the electrocatalyst displayed remarkable durability, sustaining stable performance for a duration exceeding 248 h. The significant improvement in durability provides highly valuable insights for the practical application of metal-nitrogen-carbon electrocatalysts.
Structure of HIV-1 RRE stem-loop II identifies two conformational states of the high-affinity Rev binding site
Authors: Tipo Jerricho , Gottipati Keerthi , Slaton Michael , Gonzalez-Gutierrez Giovanni , Choi Kyung H.
Published: May 2024

During HIV infection, specific RNA-protein interaction between the Rev response element (RRE) and viral Rev protein is required for nuclear export of intron-containing viral mRNA transcripts. Rev initially binds the high-affinity site in stem-loop II, which promotes oligomerization of additional Rev proteins on RRE. Here, we present the crystal structure of RRE stem-loop II in distinct closed and open conformations. The high-affinity Rev-binding site is located within the three-way junction rather than the predicted stem IIB. The closed and open conformers differ in their non-canonical interactions within the three-way junction, and only the open conformation has the widened major groove conducive to initial Rev interaction. Rev binding assays show that RRE stem-loop II has high- and low-affinity binding sites, each of which binds a Rev dimer. We propose a binding model, wherein Rev-binding sites on RRE are sequentially created through structural rearrangements induced by Rev-RRE interactions.
Wide range of possible trajectories of North Atlantic climate in a warming world
Authors: Gu Qinxue , Gervais Melissa , Danabasoglu Gokhan , Kim Who M. , Castruccio Frederic , Maroon Elizabeth , Xie Shang-Ping
Published: May 2024

Decadal variability in the North Atlantic Ocean impacts regional and global climate, yet changes in internal decadal variability under anthropogenic radiative forcing remain largely unexplored. Here we use the Community Earth System Model 2 Large Ensemble under historical and the Shared Socioeconomic Pathway 3-7.0 future radiative forcing scenarios and show that the ensemble spread in northern North Atlantic sea surface temperature (SST) more than doubles during the mid-twenty-first century, highlighting an exceptionally wide range of possible climate states. Furthermore, there are strikingly distinct trajectories in these SSTs, arising from differences in the North Atlantic deep convection among ensemble members starting by 2030. We propose that these are stochastically triggered and subsequently amplified by positive feedbacks involving coupled ocean-atmosphere-sea ice interactions. Freshwater forcing associated with global warming seems necessary for activating these feedbacks, accentuating the impact of external forcing on internal variability. Further investigation on seven additional large ensembles affirms the robustness of our findings. By monitoring these mechanisms in real time and extending dynamical model predictions after positive feedbacks activate, we may achieve skillful long-lead North Atlantic decadal predictions that are effective for multiple decades.
Para-selective nitrobenzene amination lead by C(sp2)-H/N-H oxidative cross-coupling through aminyl radical
Authors: Zhang Zhen , Yue Shusheng , Jin Bo , Yang Ruchun , Wang Shengchun , Zhang Tianqi , Sun Li , Lei Aiwen , Cai Hu
Published: May 2024

Arylamines, serving as crucial building blocks in natural products and finding applications in multifunctional materials, are synthesized on a large scale via an electrophilic nitration/reduction sequence. However, the current methods for aromatic C–H amination have not yet attained the same level of versatility as electrophilic nitration. Here we show an extensively investigated transition metal-free and regioselective strategy for the amination of nitrobenzenes, enabling the synthesis of 4-nitro-N-arylamines through C(sp2)-H/N-H cross-coupling between electron-deficient nitroarenes and amines. Mechanistic studies have elucidated that the crucial aspects of these reactions encompass the generation of nitrogen radicals and recombination of nitrobenzene complex radicals. The C(sp2)-N bond formation is demonstrated to be highly effective for primary and secondary arylamines as well as aliphatic amines under mild conditions, exhibiting exceptional tolerance towards diverse functional groups in both nitroarenes and amines (>100 examples with yields up to 96%). Notably, this C(sp2)-H/N-H cross-coupling exhibits exclusive para-selectivity.
Shaping of microbial phenotypes by trade-offs
Authors: Zhu Manlu , Dai Xiongfeng
Published: May 2024

Growth rate maximization is an important fitness strategy for microbes. However, the wide distribution of slow-growing oligotrophic microbes in ecosystems suggests that rapid growth is often not favored across ecological environments. In many circumstances, there exist trade-offs between growth and other important traits (e.g., adaptability and survival) due to physiological and proteome constraints. Investments on alternative traits could compromise growth rate and microbes need to adopt bet-hedging strategies to improve fitness in fluctuating environments. Here we review the mechanistic role of trade-offs in controlling bacterial growth and further highlight its ecological implications in driving the emergences of many important ecological phenomena such as co-existence, population heterogeneity and oligotrophic/copiotrophic lifestyles.
A reactive neural network framework for water-loaded acidic zeolites
Authors: Erlebach Andreas , Šípka Martin , Saha Indranil , Nachtigall Petr , Heard Christopher J. , Grajciar Lukáš
Published: May 2024

Under operating conditions, the dynamics of water and ions confined within protonic aluminosilicate zeolite micropores are responsible for many of their properties, including hydrothermal stability, acidity and catalytic activity. However, due to high computational cost, operando studies of acidic zeolites are currently rare and limited to specific cases and simplified models. In this work, we have developed a reactive neural network potential (NNP) attempting to cover the entire class of acidic zeolites, including the full range of experimentally relevant water concentrations and Si/Al ratios. This NNP has the potential to dramatically improve sampling, retaining the (meta)GGA DFT level accuracy, with the capacity for discovery of new chemistry, such as collective defect formation mechanisms at the zeolite surface. Furthermore, we exemplify how the NNP can be used as a basis for further extensions/improvements which include data-efficient adoption of higher-level (hybrid) references via Δ-learning and the acceleration of rare event sampling via automatic construction of collective variables. These developments represent a significant step towards accurate simulations of realistic catalysts under operando conditions.
Cerebral microstructural alterations in Post-COVID-condition are related to cognitive impairment, olfactory dysfunction and fatigue
Authors: Hosp Jonas A. , Reisert Marco , Dressing Andrea , Götz Veronika , Kellner Elias , Mast Hansjörg , Arndt Susan , Waller Cornelius F. , Wagner Dirk , Rieg Siegbert , Urbach Horst , Weiller Cornelius , Schröter Nils , Rau Alexander
Published: May 2024

After contracting COVID-19, a substantial number of individuals develop a Post-COVID-Condition, marked by neurologic symptoms such as cognitive deficits, olfactory dysfunction, and fatigue. Despite this, biomarkers and pathophysiological understandings of this condition remain limited. Employing magnetic resonance imaging, we conduct a comparative analysis of cerebral microstructure among patients with Post-COVID-Condition, healthy controls, and individuals that contracted COVID-19 without long-term symptoms. We reveal widespread alterations in cerebral microstructure, attributed to a shift in volume from neuronal compartments to free fluid, associated with the severity of the initial infection. Correlating these alterations with cognition, olfaction, and fatigue unveils distinct affected networks, which are in close anatomical-functional relationship with the respective symptoms.
Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC
Authors: Ward Nathan P. , Yoon Sang Jun , Flynn Tyce , Sherwood Amanda M. , Olley Maddison A. , Madej Juliana , DeNicola Gina M.
Published: May 2024

Cysteine metabolism occurs across cellular compartments to support diverse biological functions and prevent the induction of ferroptosis. Though the disruption of cytosolic cysteine metabolism is implicated in this form of cell death, it is unknown whether the substantial cysteine metabolism resident within the mitochondria is similarly pertinent to ferroptosis. Here, we show that despite the rapid depletion of intracellular cysteine upon loss of extracellular cystine, cysteine-dependent synthesis of Fe-S clusters persists in the mitochondria of lung cancer cells. This promotes a retention of respiratory function and a maintenance of the mitochondrial redox state. Under these limiting conditions, we find that glutathione catabolism by CHAC1 supports the mitochondrial cysteine pool to sustain the function of the Fe-S proteins critical to oxidative metabolism. We find that disrupting Fe-S cluster synthesis under cysteine restriction protects against the induction of ferroptosis, suggesting that the preservation of mitochondrial function is antagonistic to survival under starved conditions. Overall, our findings implicate mitochondrial cysteine metabolism in the induction of ferroptosis and reveal a mechanism of mitochondrial resilience in response to nutrient stress.
Combination of UGT1A1 polymorphism and baseline plasma bilirubin levels in predicting the risk of antipsychotic-induced dyslipidemia in schizophrenia patients
Authors: Lin Chenquan , Zhang Shuangyang , Yang Ping , Zhang Bikui , Guo Wenbin , Wu Renrong , Liu Yong , Wang Jianjian , Wu Haishan , Cai Hualin
Published: May 2024

The prolonged usage of atypical antipsychotic drugs (AAPD) among individuals with schizophrenia often leads to metabolic side effects such as dyslipidemia. These effects not only limit one’s selection of AAPD but also significantly reduce compliance and quality of life of patients. Recent studies suggest that bilirubin plays a crucial role in maintaining lipid homeostasis and may be a potential pre-treatment biomarker for individuals with dyslipidemia. The present study included 644 schizophrenia patients from two centers. Demographic and clinical characteristics were collected at baseline and 4 weeks after admission to investigate the correlation between metabolites, episodes, usage of AAPDs, and occurrence of dyslipidemia. Besides, we explored the combined predictive value of genotypes and baseline bilirubin for dyslipidemia by employing multiple PCR targeted capture techniques to sequence two pathways: bilirubin metabolism-related genes and lipid metabolism-related genes. Our results indicated that there existed a negative correlation between the changes in bilirubin levels and triglyceride (TG) levels in patients with schizophrenia. Among three types of bilirubin, direct bilirubin in the baseline (DBIL-bl) proved to be the most effective in predicting dyslipidemia in the ROC analysis (AUC = 0.627, p < 0.001). Furthermore, the odds ratio from multinomial logistic regression analysis showed that UGT1A1*6 was a protective factor for dyslipidemia (ß = −12.868, p < 0.001). The combination of baseline DBIL and UGT1A1*6 significantly improved the performance in predicting dyslipidemia (AUC = 0.939, p < 0.001). Schizophrenia patients with UGT1A1*6 mutation and a certain level of baseline bilirubin may be more resistant to dyslipidemia and have more selections for AAPD than other patients.
Sweet-spot operation of a germanium hole spin qubit with highly anisotropic noise sensitivity
Authors: Hendrickx N. W. , Massai L. , Mergenthaler M. , Schupp F. J. , Paredes S. , Bedell S. W. , Salis G. , Fuhrer A.
Published: May 2024

Spin qubits defined by valence band hole states are attractive for quantum information processing due to their inherent coupling to electric fields, enabling fast and scalable qubit control. Heavy holes in germanium are particularly promising, with recent demonstrations of fast and high-fidelity qubit operations. However, the mechanisms and anisotropies that underlie qubit driving and decoherence remain mostly unclear. Here we report the highly anisotropic heavy-hole g-tensor and its dependence on electric fields, revealing how qubit driving and decoherence originate from electric modulations of the g-tensor. Furthermore, we confirm the predicted Ising-type hyperfine interaction and show that qubit coherence is ultimately limited by 1/f charge noise, where f is the frequency. Finally, operating the qubit at low magnetic field, we measure a dephasing time of $${T}_{2}^{* }$$

T

2

*

 = 17.6 μs, maintaining single-qubit gate fidelities well above 99% even at elevated temperatures of T > 1 K. This understanding of qubit driving and decoherence mechanisms is key towards realizing scalable and highly coherent hole qubit arrays.
Bidirectional epigenetic editing reveals hierarchies in gene regulation
Authors: Pacalin Naomi M. , Steinhart Zachary , Shi Quanming , Belk Julia A. , Dorovskyi Dmytro , Kraft Katerina , Parker Kevin R. , Shy Brian R. , Marson Alexander , Chang Howard Y.
Published: May 2024

CRISPR perturbation methods are limited in their ability to study non-coding elements and genetic interactions. In this study, we developed a system for bidirectional epigenetic editing, called CRISPRai, in which we apply activating (CRISPRa) and repressive (CRISPRi) perturbations to two loci simultaneously in the same cell. We developed CRISPRai Perturb-seq by coupling dual perturbation gRNA detection with single-cell RNA sequencing, enabling study of pooled perturbations in a mixed single-cell population. We applied this platform to study the genetic interaction between two hematopoietic lineage transcription factors, SPI1 and GATA1, and discovered novel characteristics of their co-regulation on downstream target genes, including differences in SPI1 and GATA1 occupancy at genes that are regulated through different modes. We also studied the regulatory landscape of IL2 (interleukin-2) in Jurkat T cells, primary T cells and chimeric antigen receptor (CAR) T cells and elucidated mechanisms of enhancer-mediated IL2 gene regulation. CRISPRai facilitates investigation of context-specific genetic interactions, provides new insights into gene regulation and will enable exploration of non-coding disease-associated variants.
Research on trajectory control technology for L-shaped horizontal exploration wells in coalbed methane
Authors: Liu Xiugang , Jiang Zaibing , Wang Yi , Mo Haitao , Li Haozhe , Guo Jianlei
Published: May 2024

Horizontal wells have significant advantages in coal bed methane exploration and development blocks. However, its application in new exploration and development blocks could be challenging. Limited geological data, uncertain geological conditions, and the emergence of micro-faults in pre-drilled target coal seams make it hard to accurately control the well trajectory. The well trajectory prior to drilling needs to be optimized to ensure that the drilling trajectory is within the target coal seam and to prevent any reduction in drilling ratio (defined here as the percentage of the drilling trajectory in the entire horizontal section of the well located in the target coal seam) caused by faults. In this study, the well trajectory optimization is achieved by implementing the following process to drill pilot hole, acquire 2D resonance, and azimuthal gamma logging while drilling. The pilot hole drilling can obtain the characteristic parameters of the target coal seam and the top and bottom rock layers in advance, which can provide judgment values for the landing site design and real-time monitoring of whether the wellbore trajectory extends along the target coal seam; 2D resonance exploration can obtain the construction of set orientation before drilling and the development of small faults and formation fluctuations in the horizontal section, which can optimize the well trajectory in advance; the azimuth gamma logging while drilling technology can monitor the layers drilled by the current drill bit in real time, and can provide timely and accurate well trajectory adjustment methods.The horizontal well-Q in the Block-W of the Qinshui Basin was taken as a case study and underwent technical mechanism research and applicability analysis. The implementation of this new innovative process resulted in a successful drilling of a 711 m horizontal section, with a target coal seam drilling rate of 80%. Compared to previous L-type wells, the drilling rate increased by about 20%, and the drilling cycle shortened by 25%. The technical experience gained from this successful case provides valuable insight for low-cost exploration and development of new coalbed methane blocks.
Optimized multi-frequency nonlinear broadband piezoelectric energy harvester designs
Authors: Elgamal Mohamed A. , Elgamal Hassan , Kouritem Sallam A.
Published: May 2024

Many electrical devices can be powered and operated by harvesting the wasted energy of the surroundings. This research aims to overcome the challenges of output power with a sharp peak, small bandwidth, and the huge dimensions of the piezoelectric energy harvesters relative to the output power. The aforementioned challenges motivated us to investigate the effect of nonlinearity in the shape (tapered and straight cross-section area) as well as the fixation method (the number of fastened ends) to determine the optimal design with high output power and wide working frequency. This research proposes a novel piezoelectric energy harvester array, where each beam is made up of three fixed beams that are joined together by a center mass. The proposed design produces an output power of 35 mW between 25 and 40 Hz. The output power of the proposed design is 3.24 times more than the conventional designs. The recommended approach is simulated utilizing finite element analysis FEA. Analytical and experimental methods validate the proposed FEA, which exhibits excellent agreement.