bluuebunny/crossref_metadata_2025 huggingface dataset:
Rethinking China’s ‘economic coercion’: The case of the UK leaders’ meeting with the Dalai Lama in 2012
Authors: Zhang Biao
Published: October 2022

In 2012, David Cameron met the Dalai Lama. In retaliation for the meeting, China froze bilateral relations for 18 months. Subsequently, Cameron pledged to have no more meetings with the Dalai Lama and reiterated British recognition of Chinese sovereignty over Tibet. For some, China used economic punishment to extract the UK’s concession. For others, China used only diplomatic punishment. This article argues that both sides have underestimated the complexity of the case and need to integrate their insights for a better explanation. While China did not impose or threaten economic sanctions on the UK, its diplomatic sanctions put both political and economic pressure on the UK to concede. This article contributes to the studies of China–UK relations and China’s ‘economic coercion’.
From individual affectedness to collective identity: personal testimony campaigns on social media and the logic of collection
Authors: Gerbaudo Paolo
Published: October 2022

In recent years, there has been much debate about the consequences of the internet and social media for activism and social movements. According to Lance W Bennett and Alexandra Segerberg, digital activism involves a logic of ‘connective action’, in which collective action and collective identity lose importance. To assess the validity of this theory, I focus on personal testimony campaigns that have by now become a familiar digital tactic, especially in online mobilisations around issues of gender and sexuality. Drawing on discourse analysis of some of the most prominent examples, from #MeToo to #GirlsLikeUs, I argue that more than a logic of connection, what is at stake here is a ‘logic of collection’, involved in gathering personal testimonies as specimens of various grievances people are affected by (sexual harassment, discrimination, etc.). Aggregating personal testimonies around shared hashtags provides a means to construct and/or trasform the collective identity of the groups involved in order to raise their self-awareness and place them in a better position to engage in collective action. These practices thus suggest the need to overcome the opposition between personal and collective identity inherent in the theory of ‘connective action’, and refocus research on the forms of online identification that connect these two levels.
Experimental investigation of boundary layer flow near the cylinder wall in a direct-injection spark-ignition engine using particle image velocimetry
Authors: Tian Fuquan , Wang Tianyou , Che Zhizhao , Sun Kai , Lu Zhen , Liu Daming , Shi Lei
Published: October 2022

In a direct-injection spark-ignition (DISI) optical engine with a constant motored speed of 800 rpm, particle image velocimetry measurements on the in-cylinder bulk flow and the boundary layer flow near the cylinder wall were performed during the intake and compression strokes. Two different tumble flow levels were explored using a flap inserted in the intake port. The near-wall flow pattern under the effect of the bulk flow is more stable when the flap is fully closed with a higher intensity tumble. The serious optical distortion in the near-cylinder wall region under high magnification was addressed, and a high resolution of 76 μm × 76 μm within a field of view of 5 mm × 5 mm was achieved in six different measurement regions. Using inner scaling parameters (friction velocity uτ and viscous length-scale δυ), the dimensionless ensemble-averaged wall-tangential velocity profiles exhibit good consistency with the law-of-the-wall in the viscous sublayer but considerably departure in the logarithmic region. According to the low friction Reynolds number, the absence of the logarithmic layer was mainly attributed to the complete overlap between the viscosity-affected inner region and the outer region of the boundary layer. The near-wall velocity profiles were also normalized by three different outer scaling parameters. The collapse is significantly improved for y/ δ >0.07 when the velocity profiles are normalized by δ/ δ*(1− u/ u∞). The fluctuation RMS velocity in the wall-normal direction is greater, particularly for the high tumble flow level condition.
The novice nose knows: How olfactory knowledge shapes evaluation confidence
Authors: Meng Hua , Zamudio César , Grigsby Jamie L.
Published: October 2022

Prior consumer knowledge research compares differences between experts and novices within a product category, implicitly assuming that novices are similar across categories. Relying on the differences between incidental and intentional learning, as well as on the unique physiological characteristics of olfaction processing, we examine this assumption, comparing novice consumers between olfactory and non-olfactory categories and suggesting that olfactory novices are unique. Using electronic word-of-mouth (eWOM) analysis and experiments, we find that olfactory novices are more familiar with technical terms than non-olfactory novices in their product category. Thus, when exposed to technical olfactory product descriptions, olfactory novices are more confident in evaluating products by gaining a higher sense of understanding. This cross-category novice comparison and the underlying cognitive mechanism are new to the literature. The research highlights the theoretical importance of how olfactory novices are unique and shows how eWOM and experimental data can be combined in sensory marketing. Practically, we suggest scented product managers to provide more technical product descriptions for olfactory novices, especially when product trial is not feasible (e.g., online purchases).
Tool and formability multi-response optimization for ultimate strength and ductility of AA8011 during axial compression
Authors: Ravichandran Saravanan , Duraiswamy Rajenthirakumar , Arockiasamy Felix Sahayaraj
Published: October 2022

Friction stir incremental forming is a dieless forming process achieved through the severe plastic deformation of sheet metals by localized heat through tool friction with sheet metal. Tool rotation contributes significantly to determining the local heat generation and deformation of the sheets. In this study, a novel attempt was made to improve the desired surface quality with minimum tool wear using three different tool coatings. To identify the optimal parameters, process factors such as spindle speed, step size, and table feed were considered with two response parameters forming force and surface roughness. A total of 27 experiments based on a full factorial design were performed. During this optimization using TOPSIS, parameters are selected based on which FSIF parts should attain the minimum forming force and surface roughness. Based on the results obtained, the titanium nitride coating in the tool enhanced the surface roughness and increased the maximum formability. This study provides an optimal parameter setting for AA8011 and an understanding of the friction stir effect during the incremental forming process.
Environmental Requirements for Fungi
Authors: Janinska Barbara
Published: October 2022

This paper is the result of a literature review on environmental requirements for fungal growth indoors. Popular and not quite precise opinions on the “high humidity of the environment” necessary for fungal growth have been clarified by information on their environmental requirements (for instance, water activity) contained in a variety of publications.
Strength, bulk and surgery time of ex situ versus in situ flexor tendon repair in a cadaveric model
Authors: Lindsay Christopher , Watson Nicole , Yanik John , Glass Natalie , Fowler Timothy
Published: October 2022

The purpose of this study was to compare strength, bulk and time of repair of human cadaveric flexor digitorum profundus tendons repaired in situ versus ex situ. Ninety-six human cadaveric flexor digitorum profundus tendons were transected 5 mm distal to the A2-pulley and randomized to 2-strand, 4-strand or 6-strand repairs. We found no significant differences in repair strength between in situ and ex situ repairs, but repair strength increased with increasing number of strands. The cross-sectional area of the repair was not significantly related to the number of strands, but 4- and 6-strand in situ repairs were bulkier than ex situ repairs. In situ repair took longer, and repair time increased with increasing number of strands. We suggest that cadaveric studies of flexor tendon repair should be performed in situ in order to better mimic the clinical reality of tissue handling and repair bulkiness.
Acceptability of cannabidiol in patients with psychosis
Authors: Chesney Edward , Lamper Doga , Lloyd Millie , Oliver Dominic , Hird Emily , McGuire Philip
Published: October 2022

Background:

Cannabidiol (CBD) is a promising novel candidate treatment for psychosis. It has a more benign side effect profile than antipsychotic medications, and being treated with CBD is not perceived as being stigmatising. These observations suggest that patients with psychosis would find CBD to be a relatively acceptable treatment.

Objective:

This study tested the above hypothesis by assessing the views of a sample of patients.

Methods:

Patients with a psychotic disorder were invited to complete a survey exploring their expectations about the efficacy and side effects of CBD.

Results:

Seventy patients completed the survey. The majority (86%) were willing to try CBD as a treatment. Most patients believed that CBD would improve their psychotic symptoms (69%) and that it would have fewer side effects than their current medication (64%; mainly antipsychotics). A minority of patients (10%) were concerned that CBD might exacerbate their psychotic symptoms. This, however, appeared to reflect confusion between the effects of CBD and those of cannabis.

Conclusion:

Most patients with psychosis regard CBD as an acceptable treatment. Although CBD has not yet been approved as a treatment for psychosis, many patients are aware of it through the presence of CBD in cannabis and in health supplements. When added to the emerging evidence of its efficacy and the low risk of side effects, the high acceptability of CBD underlines its therapeutic potential.
Pediatric youth who have obesity have high rates of adult criminal behavior and low rates of homeownership
Authors: Powell Adam W , Siegel Zachary , Kist Christopher , Mays Wayne A , Kharofa Roohi , Siegel Robert
Published: October 2022

Objectives:

The social outcomes in adulthood for pediatric patients with obesity are not well-described. This study investigated lifetime criminal behavior and homeownership in youth with obesity.

Methods:

Retrospective data on all children enrolled in the weight management program from 1999 to 2009 and who completed exercise testing were collected. Demographic and public record collection included body habitus, death records, real estate transactions, and criminal conviction history with comparisons made to published normative data.

Results:

In the children with obesity studied ( N = 716; 12.0 ± 3.1 years old), the now-adult patients (28.5 ± 3.7 years) had a 1.5% mortality rate (11/716). Overall, 9.6% of these adults were convicted of a felony compared to ~7% lifetime prevalence in Ohio ( p = 0.03). Also, 14.7% of study patients purchased a home compared to 38.3% of Midwest adults <30 years old ( p < 0.0001). Mortality, history of a criminal conviction, or homeownership was associated with any exercise or study parameter.

Conclusion:

Children with obesity appear to have greater social risk than their peers in adulthood with higher rates of criminal behavior and lower rates of homeownership. This appears to highlight the need for treatment in this vulnerable group of children and young adults.
Erythrodermic mycosis fungoides with large cell transformation: An unusual and complicated case
Authors: Lombardi Conner V , Glosser Logan D , Hopper Wade , Veria Spiro , Awad Mohammed T , Garg Anu
Published: October 2022

Mycosis fungoides is the most common cutaneous T-cell lymphoma. It presents a diagnostic challenge due to resemblance with many other dermatologic conditions. The disease typically follows a progression from patches to plaques to skin-based tumors with potential for visceral involvement. Diagnosis is made by clinical presentation and histology. When early diagnosis is made, there is an estimated 88% five-year survival. This report details a 60-year-old Black man diagnosed with stage IIIA mycosis fungoides with a severe degree of cutaneous involvement. This case is unique due to the aggressive large cell transformation and rapid progression to death within 18 months of diagnosis. We highlight the challenge of diagnosing, treating, and monitoring the therapeutic response of mycosis fungoides. Finally, this case calls for a multi-disciplinary approach to treatment and to include mycosis fungoides on the differential diagnosis for patients presenting with a variety of vague, recurrent cutaneous symptoms, especially with patchy dyspigmentation or plaques.
Severe hyponatremia due to trimethoprim-sulfamethoxazole-induced SIADH
Authors: Marak Creticus , Nunley Matthew , Guddati Achuta Kumar , Kaushik Prashant , Bannon Mark , Ashraf Adrita
Published: October 2022

Hyponatremia, a serum sodium level of <135 mEq/L, is the most common electrolyte abnormality occurring in 5%–35% of hospitalized patients. It is a predictor of increased morbidity and mortality. Diuretics, psychotropic, and antiepileptic drugs are commonly implicated in drug-induced hyponatremia. Trimethoprim-sulfamethoxazole and spironolactone are two commonly prescribed drugs; unfortunately, most providers are unfamiliar with these two drugs causing hyponatremia. Simultaneous use of trimethoprim-sulfamethoxazole and spironolactone can cause serious drug interactions that increase the risk of hyponatremia, hyperkalemia, and overall mortality. Despite recommendations to avoid using these two drugs concurrently, many healthcare providers continue to prescribe them together. We report a case of an elderly female with severe hyponatremia caused by trimethoprim-sulfamethoxazole superimposed on a chronic but stable mild hyponatremia.
Toward the renal vesicle: Ultrastructural investigation of the cap mesenchyme splitting process in the developing kidney
Authors: Piras Monica , Gerosa Clara , Congiu Terenzio , Cau Flaviana , Fanni Daniela , Pichiri Giuseppina , Coni Pierpaolo , Lachowicz Joanna Izabela , Schirru Enrico , Congia Mauro , Rossino Rossano , Muntoni Sandro , Jaremko Mariusz , Piludu Marco
Published: October 2022

Background:

A complex sequence of morphogenetic events leads to the development of the adult mouse kidney. In the present study, we investigated the morphological events that characterize the early stages of the mesenchymal-to-epithelial transition of cap mesenchymal cells, analyzing in depth the relationship between cap mesenchymal induction and ureteric bud (UB) branching.

Design and methods:

Normal kidneys of newborn non-obese diabetic (NOD) mice were excised and prepared for light and electron microscopic examination.

Results:

Nephrogenesis was evident in the outer portion of the renal cortex of all examined samples. This process was mainly due to the interaction of two primordial derivatives, the ureteric bud and the metanephric mesenchyme. Early renal developmental stages were initially characterized by the formation of a continuous layer of condensed mesenchymal cells around the tips of the ureteric buds. These caps of mesenchymal cells affected the epithelial cells of the underlying ureteric bud, possibly inducing their growth and branching.

Conclusions:

The present study provides morphological evidence of the reciprocal induction between the ureteric bud and the metanephric mesenchyme showing that the ureteric buds convert mesenchyme to epithelium that in turn stimulates the growth and the branching of the ureteric bud.
Knowledge, attitude, and practice toward the COVID-19 infection among adults Iran: A cross-sectional study
Authors: NeJhaddadgar Nazila , Pirani Narges , Heydarian Nasrin , Ebadi Fard Azar Amir Ali , Yazdi Fateme , Toghroli Razie , Chaboksavar Fakhreddin , Shalchi Oghli Somayyeh , Kianipour Neda , Zokaei Abdolhamid , Foroughinia Azadeh
Published: October 2022

Background:

Coronavirus illness (COVID-19) has spread globally and is affecting everyone severely. The evaluation of educational needs (knowledge, attitudes, and practices) is important in controlling COVID-19 situations. The goal of this study is to find out what adults in Ardabil City know, how they feel, and what they do about the COVID-19 infection.

Methods:

In November 2021, a cross-sectional descriptive-correlational survey of 384 people was conducted using stratified-cluster sampling in Ardabil. The researchers created a self-reported questionnaire with 23 items as the data collection technique. The quantitative data were evaluated using descriptive statistics, the chi-square test, the correlation coefficient, and regression analysis.

Results:

The correct answer rate for this research found that 73.17% of participants ( n = 281) had appropriate knowledge, 61.19% ( n = 235) had favorable attitudes, and 69.53% ( n = 267) had enough practice behavior. However, knowledge was related to gender, employment, and location of residence. Age, marital status, education level, and location of residence were all connected with attitude. Age, gender, and marital status were all related to the behavior. The findings of linear regression analysis revealed that knowledge and attitude influence behavior.

Conclusion:

The study findings revealed a high degree of understanding of COVID-19, a positive attitude, and a strong commitment to good practices. Knowledge, attitudes, and behaviors were influenced to varying degrees by age, marital status, education level, employment, and location of residence. Furthermore, knowledge and attitudes influenced behaviors.
Pathological Thresholds of Segmental Femoral Torsion in Patients With Patellar Dislocation: Influence on Patellofemoral Malalignment
Authors: Xu Zijie , Song Yifan , Deng Ronghui , Zhang Zining , Wang Haijun , Yu Jia-Kuo
Published: October 2022

Background

Femoral torsion can be evaluated from different femoral segments. The pathological thresholds for femoral torsion of different segments and the influence of segmental femoral torsion on patellofemoral alignment remain unknown.

Purpose

To compare femoral torsion between patients with recurrent patellar dislocation and healthy individuals, to determine the statistical physiological range and pathological thresholds of femoral torsion in different segments, and to investigate the influence of femoral torsion on patellofemoral malalignment.

Study Design

Cross-sectional study; Level of evidence, 3.

Methods

We retrospectively reviewed the records of patients with patellar dislocation who received surgical treatment in our department between 2019 and 2020. Healthy participants were recruited as the control group. The control patients were asymptomatic and had no history of lower extremity disorders. The differences in femoral torsion between the study and control groups were compared. The diagnostic capacity of femoral torsion in different segments and their correlation with patellar tilt were investigated. The mean value and 95% CI of femoral torsion in different segments were established using data from healthy volunteers.

Results

A total of 60 patients with patellar dislocation and 100 healthy volunteers were included in this study. The total, mid, and distal femoral torsion values differed significantly between the study and control groups ( P < .01). Total femoral torsion had the highest diagnostic value (area under the receiver operating curve = 0.733). Total torsion ( r = 0.432; P < .001), mid torsion ( r = 0.242; P = .002), and distal torsion ( r = 0.324; P < .001) showed significant correlations with patellar tilt. The pathological thresholds of excessive femoral torsion of the total, proximal, mid, and distal femoral segments were 24.73°, 46.68°, –6.55°, and 14.92°, respectively.

Conclusion

Patients with patellar dislocation had greater femoral torsion than healthy individuals in multiple femoral segments. Excessive mid, distal, and total torsion was associated with more significant patellar tilt.
Comparison of Functional Outcomes After Arthroscopic Rotator Cuff Repair Between Patients With Traumatic and Atraumatic Tears
Authors: Godshaw Brian M. , Hughes Jonathan D. , Boden Stephanie Ann , Lin Albert , Lesniak Bryson P.
Published: October 2022

Background:

The role of tear etiology in outcomes after rotator cuff repair is not well understood.

Purpose/Hypothesis:

The purpose of this study was to determine the difference in outcomes after rotator cuff repair based on tear etiology. We hypothesized that traumatic rotator cuff tears will have greater improvements in functional outcome measures and range of motion (ROM) than atraumatic tears.

Study Design:

Cohort study; Level of evidence, 3.

Methods:

We conducted a chart review of 221 consecutive patients who underwent arthroscopic rotator cuff repair; prospectively collected preoperative and minimum 2-year postoperative data were evaluated. Shoulder ROM, strength, and standard shoulder physical examination findings were recorded pre- and postoperatively. Outcome measures included visual analog scale for pain, Subjective Shoulder Value (SSV), 10-item Patient-Reported Outcomes Measurement Information System (PROMIS-10; physical and mental components), and American Shoulder and Elbow Surgeons (ASES) form.

Results:

Of the 221 patients, 73 had traumatic tears and 148 had atraumatic/degenerative tears. There were no differences in age, body mass index, or Charlson Comorbidity Index between groups. Patients in the atraumatic cohort had significantly longer duration of symptoms before presentation (18 vs 7 months; P < .01). Preoperatively, the traumatic cohort had less motion to forward flexion (mean ± SD; 138° ± 43.7° vs 152° ± 29.8°; P = .02). Postoperatively, both groups experienced significant improvements in visual analog scale and SSV scores ( P < .001 each). However, only the traumatic cohort demonstrated improvements in ASES and PROMIS-10 physical component scores. Patients with traumatic rotator cuff tears had lower preoperative SSV and less motion than those with atraumatic tears, but they had greater improvements in SSV (40.6% ± 39.0% vs 29.2% ± 39.7%; P = .005) and forward flexion (21.6° ± 48.6° vs 2.3° ± 48.2°; P < .001), as well as strength in forward flexion, external rotation, and internal rotation ( P < .001, P = .003, and P = .002, respectively).

Conclusion:

Patients with traumatic rotator cuff tears have worse preoperative symptoms and more functional deficits but experience greater improvements in ROM, strength, and perceived shoulder function than those with degenerative/atraumatic tears.
IL-13/IL-4 signaling contributes to fibrotic progression of the myeloproliferative neoplasms
Authors: Melo-Cardenas Johanna , Bezavada Lavanya , Crawford Jeremy Chase , Gurbuxani Sandeep , Cotton Anitria , Kang Guolian , Gossett Jeffrey , Marinaccio Christian , Weinberg Rona , Hoffman Ronald , Migliaccio Anna Rita , Zheng Yan , Derecka Marta , Rinaldi Ciro R. , Crispino John D.
Published: October 2022

Myelofibrosis (MF) is a disease associated with high unmet medical needs because allogeneic stem cell transplantation is not an option for most patients, and JAK inhibitors are generally effective for only 2 to 3 years and do not delay disease progression. MF is characterized by dysplastic megakaryocytic hyperplasia and progression to fulminant disease, which is associated with progressively increasing marrow fibrosis. Despite evidence that the inflammatory milieu in MF contributes to disease progression, the specific factors that promote megakaryocyte growth are poorly understood. Here, we analyzed changes in the cytokine profiles of MF mouse models before and after the development of fibrosis, coupled with the analysis of bone marrow populations using single-cell RNA sequencing. We found high interleukin 13 (IL-13) levels in the bone marrow of MF mice. IL-13 promoted the growth of mutant megakaryocytes and induced surface expression of transforming growth factor β and collagen biosynthesis. Similarly, analysis of samples from patients with MF revealed elevated levels of IL-13 in the plasma and increased IL-13 receptor expression in marrow megakaryocytes. In vivo, IL-13 overexpression promoted disease progression, whereas reducing IL-13/IL-4 signaling reduced several features of the disease, including fibrosis. Finally, we observed an increase in the number of marrow T cells and mast cells, which are known sources of IL-13. Together, our data demonstrate that IL-13 is involved in disease progression in MF and that inhibition of the IL-13/IL-4 signaling pathway might serve as a novel therapeutic target to treat MF.
PreAcrs: a machine learning framework for identifying anti-CRISPR proteins
Authors: Zhu Lin , Wang Xiaoyu , Li Fuyi , Song Jiangning
Published: October 2022

Background

Anti-CRISPR proteins are potent modulators that inhibit the CRISPR-Cas immunity system and have huge potential in gene editing and gene therapy as a genome-editing tool. Extensive studies have shown that anti-CRISPR proteins are essential for modifying endogenous genes, promoting the RNA-guided binding and cleavage of DNA or RNA substrates. In recent years, identifying and characterizing anti-CRISPR proteins has become a hot and significant research topic in bioinformatics. However, as most anti-CRISPR proteins fall short in sharing similarities to those currently known, traditional screening methods are time-consuming and inefficient. Machine learning methods could fill this gap with powerful predictive capability and provide a new perspective for anti-CRISPR protein identification.

Results

Here, we present a novel machine learning ensemble predictor, called PreAcrs, to identify anti-CRISPR proteins from protein sequences directly. Three features and eight different machine learning algorithms were used to train PreAcrs. PreAcrs outperformed other existing methods and significantly improved the prediction accuracy for identifying anti-CRISPR proteins.

Conclusions

In summary, the PreAcrs predictor achieved a competitive performance for predicting new anti-CRISPR proteins in terms of accuracy and robustness. We anticipate PreAcrs will be a valuable tool for researchers to speed up the research process. The source code is available at:https://github.com/Lyn-666/anti_CRISPR.git.
Structural and developmental dynamics of Matrix associated regions in Drosophila melanogaster genome
Authors: Sureka Rahul , Avvaru Akshay Kumar , Sowpati Divya Tej , Pathak Rashmi Upadhyay , Mishra Rakesh Kumar
Published: October 2022

Background

Eukaryotic genome is compartmentalized into structural and functional domains. One of the concepts of higher order organization of chromatin posits that the DNA is organized in constrained loops that behave as independent functional domains. Nuclear Matrix (NuMat), a ribo-proteinaceous nucleoskeleton, provides the structural basis for this organization. DNA sequences located at base of the loops are known as the Matrix Attachment Regions (MARs). NuMat relates to multiple nuclear processes and is partly cell type specific in composition. It is a biochemically defined structure and several protocols have been used to isolate the NuMat where some of the steps have been critically evaluated. These sequences play an important role in genomic organization it is imperative to know their dynamics during development and differentiation.

Results

Here we look into the dynamics of MARs when the preparation process is varied and during embryonic development of D. melanogaster. A subset of MARs termed as “Core-MARs” present abundantly in pericentromeric heterochromatin, are constant unalterable anchor points as they associate with NuMat through embryonic development and are independent of the isolation procedure. Euchromatic MARs are dynamic and reflect the transcriptomic profile of the cell. New MARs are generated by nuclear stabilization, and during development, mostly at paused RNA polymerase II promoters. Paused Pol II MARs depend on RNA transcripts for NuMat association.

Conclusions

Our data reveals the role of MARs in functionally dynamic nucleus and contributes to the current understanding of nuclear architecture in genomic context.
Escalating pyrethroid resistance in two major malaria vectors Anopheles funestus and Anopheles gambiae (s.l.) in Atatam, Southern Ghana
Authors: Mugenzi Leon M. J. , Akosah-Brempong Gabriel , Tchouakui Magellan , Menze Benjamin D. , Tekoh Theofelix A. , Tchoupo Micareme , Nkemngo Francis N. , Wondji Murielle J. , Nwaefuna Ekene K. , Osae Michael , Wondji Charles S.
Published: October 2022

Background

Aggravation of insecticide resistance in malaria vectors is threatening the efforts to control malaria by reducing the efficacy of insecticide-based interventions hence needs to be closely monitored. This study investigated the intensity of insecticide resistance of two major malaria vectors An. funestus sensu stricto (s.s.) and An. gambiae sensu lato (s.l.) collected in southern Ghana and assessed the bio-efficacy of several long-lasting insecticidal nets (LLINs) against these mosquito populations.

Methods

The insecticide susceptibility profiles of Anopheles funestus s.s. and Anopheles gambiae s.l. populations from Obuasi region (Atatam), southern Ghana were characterized and the bio-efficacy of some LLINs was assessed to determine the impact of insecticide resistance on the effectiveness of these tools. Furthermore, molecular markers associated with insecticide resistance in both species were characterized in the F0 and F1 populations using PCR and qPCR methods.

Results

Anopheles funestus s.s. was the predominant species and was resistant to pyrethroids, organochlorine and carbamate insecticides, but fully susceptible to organophosphates. An. gambiae s.l. was resistant to all four insecticide classes. High intensity of resistance to 5 × and 10 × the discriminating concentration (DC) of pyrethroids was observed in both species inducing a considerable loss of efficacy of long-lasting insecticidal nets (LLINs). Temporal expression analysis revealed a massive 12-fold increase in expression of the CYP6P4a cytochrome P450 gene in An. funestus s.s., initially from a fold change of 41 (2014) to 500 (2021). For both species, the expression of candidate genes did not vary according to discriminating doses. An. gambiae s.l. exhibited high frequencies of target-site resistance including Vgsc-1014F (90%) and Ace-1 (50%) while these mutations were absent in An. funestus s.s.

Conclusions

The multiple and high intensity of resistance observed in both malaria vectors highlights the need to implement resistance management strategies and the introduction of new insecticide chemistries.
NOVA1 promotes NSCLC proliferation and invasion by activating Wnt/β-catenin signaling
Authors: Qu Lianyue , Tian Yulong , Wang Fan , Li Zixuan
Published: October 2022

Background

Neuro-oncological ventral antigen 1 (NOVA1) is a neuron-specific RNA-binding protein which regulates alternative splicing in the developing nervous system. Recent research has found that NOVA1 plays a significant role in carcinogenesis. In this paper, we examine the role of NOVA1 in non-small cell lung cancer (NSCLC) and its underlying molecular mechanisms.

Methods

The expression of NOVA1 in NSCLC was detected by immunohistochemistry and correlations between NOVA1 expression and clinicopathological factors were analyzed by chi–square tests. Kaplan–Meier survival analysis and the Cox regression model were used to evaluate the predictive effect of prognostic factors. Western blotting, Cell Counting Kit-8, colony formation, apoptosis, migration and invasion assays were used to detect the effects of silencing (si)NOVA1 RNA on Wnt/β-catenin signaling and biological behavior in NSCLC cell lines.

Results

Our study showed that expression of NOVA1 was up-regulated and significantly correlated with poor differentiation (p = 0.020), advanced TNM stage (P = 0.001), T stage (P = 0.001) and lymph node metastasis (P = 0.000) as well as the expression of β-catenin (P = 0.012) in NSCLC. The down-regulation of NSCLC by siRNA significantly inhibited proliferation, migration and invasion and promoted apoptosis in NSCLC cells. Expression of Wnt signaling molecules, including β-catenin, activated β-catenin, cyclin D1, matrix metalloproteinase (MMP)-2 and MMP-7, was also significantly reduced by siNOVA1. The inhibition of Wnt/β-catenin signaling in A549 and H1299 cells by siNOVA1 was reversed after treatment with a β-catenin expression plasmid.

Conclusion

The present study suggests that NOVA1 may serve as a potential prognosis biomarker in NSCLC. High NOVA1 expression was associated with poor survival rate. Finally, in vitro experiments verified that NOVA1 promotes NSCLC cell proliferation and invasion by regulating Wnt/β-catenin signaling.
Evaluation of urinary continence status and its influence on quality of life after gyneco-oncological treatment of female pelvic malignancies at an oncological center
Authors: Zafarnia Maryam , Kennes Lieven N. , Stickeler Elmar , Hoff Johannes , Najjari Laila
Published: October 2022

Urinary incontinence (UI) could negatively affect a person’s quality of life (QoL). This study investigates the association among gynecological cancers, their treatments, UI, and its effect on the QoL of survivors of gynecological cancer. This cross-sectional questionnaire-based study included 121 cases from 405 patients who had undergone gyneco-oncological therapy. The participants were asked whether they experienced any form of UI and whether it impacted their QoL. The following therapies were used to treat the 12 gynecologic tumor types found in the participants: surgery (n = 116, 95.87%), chemotherapy (CTx) (n = 51, 42.2%), radiotherapy (RTx) (n = 31, 25.6%), and antibody therapy (ABT) (n = 11, 9.1%). No significant association was determined between tumor type and UI. However, body mass index (BMI), radical hysterectomy, vulvar or vaginal surgery, and presence of UI before treatment had significant impacts on the presence of UI. The surgical access routes, CTx, ABT, and hysterectomy had significant impacts on the severity of UI after treatment. Among all patients, 55.4% reported very good QoL. These reports of good QoL by patients could be due to their very good adjustment to the situation, with regard to being diagnosed with and receiving treatment for cancer, or due to the patients considering UI to not be much of an issue. Additionally, 34% of patients reported they had not been informed about the risk of UI before treatment. Informing patients about UI as one of the risks of therapy before initiating the treatment is crucial as patients who had been informed beforehand coped with UI far better than those who were not informed. Hence, the treatment of UI is often successful, so patients should be encouraged to receive urogynecological consultation.
Entrustable professional activities for Junior Brazilian Medical Students in community medicine
Authors: Francischetti Ieda , Holzhausen Ylva , Peters Harm
Published: October 2022

Background

Entrustable professional activities (EPAs) have been defined to promote the workplace participation of undergraduate medical students, generally in the context of high-income countries with a focus on the secondary and tertiary health care sectors. These EPAs have limited applicability to training and health care contexts in low- to middle-income countries that have a focus on primary health care, for instance, the context of community medicine. The purpose of this article is to report the process and results of defining EPAs for undergraduate medical training in a community health care setting.

Methods

A modified Delphi study was performed to develop EPAs for the training of medical students in community medicine during their first and second years of education at the Marília Medical School (FAMEMA), Brazil. The supervision level was operationalized in terms of a student’s ability to perform the EPA autonomously in an effective and safe manner with supervision readily available on request. Panellists (9 physicians and 6 nurses) rated the completeness of the proposed list of EPAs and EPA categories on four-point Likert scales. The threshold for consensus among panellists was a mean content validity index of at least 80%.

Results

Consensus was reached after two Delphi rounds, resulting in 11 EPAs for undergraduate medical education and training in community medicine. These EPAs were organized into three overarching EPA domains: integrality of care for individual health needs in all phases of the life cycle (5 EPAs), integrality of care for family health needs (3 EPAs), and integrality of care for community health needs (3 EPAs). For each EPA, descriptions of the following categories were created: title; specifications and limitations; conditions and implications of the entrustment decision; knowledge, skills, and attitudes; links to competencies; and assessment sources.

Conclusion

The resulting 11 EPAs for training medical students in community medicine expand the application of the EPA framework to both early undergraduate medical education and the context of primary health care. This report can support and guide other medical schools in their attempts to train students in primary health care contexts and to incorporate EPAs into their curricula.
Developing a competence framework for gerontological nursing in China: a two-phase research design including a needs analysis and verification study
Authors: Dijkman Bea L. , Hirjaba Marina , Wang Wenwen , Palovaara Marjo , Annen Marjolein , Varik Merle , Cui Ying’ai , Li Jing , van Slochteren Cornelia , Jihong Wang , Feiteng Chen , Luo Yu , Chen Yudong , Paans Wolter
Published: October 2022

Background

China faces a serious shortage of competent nurses who can address the healthcare needs of older people in an ageing society. Chinese higher education institutes face serious challenges when it comes to developing new curricula that are capable of educating sufficient numbers of competent gerontological nurses. Therefore, the aim of this research study was to identify and verify competencies for gerontological nurses in China that are needed to provide nursing care for the growing number of older people in all care settings. This study takes into account the possible opportunities that trends and developments may offer in the near future.

Methods

In this study, a two-phase research design was used. The first phase concerned needs analysis, including a situational analysis, a trend analysis and a competence analysis. This process resulted in a draft competence framework. The second phase addressed the verification of the competence framework through a two-round Delphi study with a panel of Chinese and European experts. This process led to the final competence framework.

Results

The final competence framework for gerontological nursing in China included six competencies divided into 13 essential and five relevant learning outcomes. The competencies are: ‘providing gerontological care’, ‘communication and collaboration’, ‘organization of gerontological nursing care’, ‘health promotion’, ‘evidence-based nursing and lifelong learning’ and ‘professional behaviour’.

Conclusion

The framework comprehensively covers the six core competencies that nurses who care for older people should possess. These competencies are well-embedded in a Chinese context. The framework therefore offers concrete, practical suggestions for the competencies and skills that nursing graduates will need to work in current and future professions related to gerontological nursing education and practice.
(Dis)advantaged positions in accessing gender-affirming healthcare in Finland: an intersectional qualitative study of foreign-origin transgender people
Authors: Czimbalmos Mercédesz , Rask Shadia
Published: October 2022

Background

An increasing body of scholarship focuses on transgender individuals’ experiences when accessing gender-affirming healthcare. However, the experiences of transgender individuals who identify as being of foreign-origin in Finland have rarely been studied. This study aims to fill the gap in research and contribute to the understanding of the experiences of transgender individuals who also identify as belonging to the foreign-origin populations in Finland.

Methods

Fourteen semi-structured qualitative interviews were conducted and analyzed with reflexive thematic analysis (RTA), through the framework of intersectionality. The interviews were part of a broader sample of qualitative data, collected about the experiences of sexual and gender minorities among the foreign-origin populations in Finland.

Results

The analysis showed two main interconnected themes. Firstly, perceived barriers when accessing gender-affirming care. In this theme, the intersections of transgender identity, foreign background, class, and age affected the experiences of the individuals. Secondly, the necessity of “performing identities:” the intersections of class, transgender identity, nativity, and race affected those.

Conclusion

The findings of the current study suggest that the intersectional aspects of individual identities create structural inequalities in the Finnish gender-affirming healthcare system. To tackle these inequalities, further research is needed on the healthcare experiences of gender minorities in Finland both within and outside the scope of transgender-specific healthcare.
Why do older people not use the public health services of the integrated aging program? A multidimensional approach in a qualitative study
Authors: Moghadasi Amir Mohamad , Sum Shima , Matlabi Hossein
Published: October 2022

Objectives

The world’s population is aging rapidly and a huge amount of services are being provided to meet the needs of the older people. Identifying the factors affecting the non-attendance of the older people to health care centres is of particular importance. We focused on the reasons why older people do not use the services of the integrated aging program in Iran from the perspective of the older people, general practitioners, and primary health providers.

Methods

A qualitative study in Ghaemshahr (IRAN) carried out during 2021. Data were collected through semi-structured interviews in two groups with the participation of 29 older adults and 18 employees of the health centres Purposeful sampling and sample size were determined based on data saturation. Data were analyzed manually using conventional content analysis.

Results

Potential barriers to and challenges of older adults were generally categorized into four main themes including individual, systemic-structural, environmental, and social factors.

Conclusions

Both groups agreed on many aspects, including lack of education of the patients and lack of proper medical services. Existing problems in health care relate to both medical and non-medical factors. Improvement in health care delivery requires a deliberate focus on the patients’ specific needs.
Metabolomic profile of prostate cancer-specific survival among 1812 Finnish men
Authors: Huang Jiaqi , Zhao Bin , Weinstein Stephanie J. , Albanes Demetrius , Mondul Alison M.
Published: October 2022

Background

Abnormal metabolism and perturbations in metabolic pathways play significant roles in the development and progression of prostate cancer; however, comprehensive metabolomic analyses of human data are lacking and needed to elucidate the interrelationships.

Methods

We examined the serum metabolome in relation to prostate cancer survival in a cohort of 1812 cases in the Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study. Using an ultrahigh-performance LC-MS/MS platform, we identified 961 known metabolites in prospectively collected serum. Median survival time from diagnosis to prostate cancer-specific death (N=472) was 6.6 years (interquartile range=2.9–11.1 years). Cox proportional hazards regression models estimated hazard ratios and 95% confidence intervals of the associations between the serum metabolites (in quartiles) and prostate cancer death, adjusted for age at baseline and diagnosis, disease stage, and Gleason sum. In order to calculate risk scores, we first randomly divided the metabolomic data into a discovery set (70%) and validated in a replication set (30%).

Results

Overall, 49 metabolites were associated with prostate cancer survival after Bonferroni correction. Notably, higher levels of the phospholipid choline, amino acid glutamate, long-chain polyunsaturated fatty acid (n6) arachidonate (20:4n6), and glutamyl amino acids gamma-glutamylglutamate, gamma-glutamylglycine, and gamma-glutamylleucine were associated with increased risk of prostate cancer-specific mortality (fourth versus first quartile HRs=2.07–2.14; P-values <5.2×10−5). By contrast, the ascorbate/aldarate metabolite oxalate, xenobiotics S-carboxymethyl-L-cysteine, fibrinogen cleavage peptides ADpSGEGDFXAEGGGVR and fibrinopeptide B (1-12) were related to reduced disease-specific mortality (fourth versus first quartile HRs=0.82–0.84; P-value <5.2×10−5). Further adjustment for years from blood collection to cancer diagnosis, body mass index, smoking intensity and duration, and serum total and high-density lipoprotein cholesterol did not alter the results. Participants with a higher metabolic score based on the discovery set had an elevated risk of prostate cancer-specific mortality in the replication set (fourth versus first quartile, HR=3.9, P-value for trend<0.0001).

Conclusions

The metabolic traits identified in this study, including for choline, glutamate, arachidonate, gamma-glutamyl amino acids, fibrinopeptides, and endocannabinoid and redox pathways and their composite risk score, corroborate our previous analysis of fatal prostate cancer and provide novel insights and potential leads regarding the molecular basis of prostate cancer progression and mortality.