bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Control of Wind Turbine Systems via Time-Scale Decomposition
Authors: Al-Iedani Intessar , Gajic Zoran
Published: January 2020

In this paper, we design an optimal controller for a wind turbine (WT) with doubly-fed induction generator (DFIG) by decomposing the algebraic Riccati equation (ARE) of the singularly perturbed wind turbine system into two reduced-order AREs that correspond to the slow and fast time scales. In addition, we derive a mathematical expression to obtain the optimal regulator gains with respect to the optimal pure-slow and pure-fast, reduced-order Kalman filters and linear quadratic Gaussian (LQG) controllers. Using this method allows the design of the linear controllers for slow and fast subsystems independently, thus, achieving complete separation and parallelism in the design process. This solves the corresponding ill-conditioned problem and reduces the complexity that arises when the number of wind turbines integrated to the power system increases. The reduced-order systems are compared to the original full-order system to validate the performance of the proposed method when a wind turbulence and a large-signal disturbance are applied to the system. In addition, we show that the similarity transformation does not preserve the performance index value in case of Kalman filter and the corresponding LQG controller.
Secure Routing Protocols for Source Node Privacy Protection in Multi-Hop Communication Wireless Networks
Authors: Mutalemwa Lilian C. , Shin Seokjoo
Published: January 2020

Traffic analysis attacks are common in monitoring wireless sensor networks (WSNs). In the attacks, adversaries analyze the traffic pattern to obtain critical information such as the location information of a source node. Fake source packet routing protocols are often used to ensure source location privacy (SLP) protection. The protocols rely on broadcasting fake packets from fake sources concurrently with the transmission of real packets from the real source nodes to obfuscate the adversaries. However, fake source packet routing protocols have demonstrated some performance limitations including high energy consumption, low packet delivery ratio (PDR), and long end-to-end delay (EED). In this study, two existing fake source packet routing protocols are considered. Then two new phantom-based SLP routing protocols are proposed to address the limitations. Each proposed protocol introduces a two-level phantom routing strategy to ensure two adversary confusion phases. When the adversaries perform traffic analysis attacks on the packet routes, they encounter two levels of obfuscation. Simulation results establish that the proposed protocols have superior performance features. The protocols guarantee strong SLP protection throughout the WSN domain with controlled energy consumption, PDR, and EED. Furthermore, the proposed protocols achieve more practical results under varied network configurations.
Multi-Horizon Dependence between Crude Oil and East Asian Stock Markets and Implications in Risk Management
Authors: Cai Xiaojing , Hamori Shigeyuki , Yang Lu , Tian Shuairu
Published: January 2020

This paper examines the dynamic dependence structure of crude oil and East Asian stock markets at multiple frequencies using wavelet and copulas. We also investigate risk management implications and diversification benefits of oil-stock portfolios by calculating and comparing risk and tail risk hedging performance. Our results provide strong evidence of time-varying dependence and asymmetric tail dependence between crude oil and East Asian stock markets at different frequencies. The level and fluctuation of their dependencies increase as time scale increases. Furthermore, we find the time-varying hedging benefits differ at investment horizons and reduced over the long run. Our results suggest that crude oil could be used as a hedge and safe haven against East Asian stock markets, especially in the short- and mid-term.
Large Scale Smart Charging of Electric Vehicles in Practice
Authors: Zweistra Marisca , Janssen Stan , Geerts Frank
Published: January 2020

The energy system is changing due to a steady increase in electric vehicles on the demand side and local production (mostly through solar panels) on the production side. Both developments can put the energy grid under stress during certain timeframes, while there might be enough capacity on the grid most of the day. Smart charging of electric vehicles might be a solution to time dependent congestion. In this study, a smart charging strategy was developed and tested in large scale with 1000 public chargers, operated in the real word. We developed and tested protocols to temporarily limit the charger capacity based on the transformer data and the number of running sessions. Over 150,000 sessions were handled, of which almost half were influenced by the smart charging strategy applied. We found that we were able to keep within the grid limits by using these controls, without hindering the driver experience. Further improvements to the smart charging strategy can be made as soon as car manufacturers share information about the car battery such as the state of charge.
Glabralysins, Potential New β-Pore-Forming Toxin Family Members from the Schistosomiasis Vector Snail Biomphalaria glabrata
Authors: Lassalle Damien , Tetreau Guillaume , Pinaud Silvain , Galinier Richard , Crickmore Neil , Gourbal Benjamin , Duval David
Published: January 2020

Biomphalaria glabrata is a freshwater Planorbidae snail. In its environment, this mollusk faces numerous microorganisms or pathogens, and has developed sophisticated innate immune mechanisms to survive. The mechanisms of recognition are quite well understood in Biomphalaria glabrata, but immune effectors have been seldom described. In this study, we analyzed a new family of potential immune effectors and characterized five new genes that were named Glabralysins. The five Glabralysin genes showed different genomic structures and the high degree of amino acid identity between the Glabralysins, and the presence of the conserved ETX/MTX2 domain, support the hypothesis that they are pore-forming toxins. In addition, tertiary structure prediction confirms that they are structurally related to a subset of Cry toxins from Bacillus thuringiensis, including Cry23, Cry45, and Cry51. Finally, we investigated their gene expression profiles in snail tissues and demonstrated a mosaic transcription. We highlight the specificity in Glabralysin expression following immune stimulation with bacteria, yeast or trematode parasites. Interestingly, one Glabralysin was found to be expressed in immune-specialized hemocytes, and two others were induced following parasite exposure.
Development and Fidelity Testing of the Test@Work Digital Toolkit for Employers on Workplace Health Checks and Opt-In HIV Testing
Authors: Blake Holly , Somerset Sarah , Evans Catrin
Published: January 2020

Background: In the UK, few employers offer general health checks for employees, and opt-in HIV testing is rarely included. There is a need to provide evidence-based guidance and support for employers around health checks and HIV testing in the workplace. An Agile approach was used to develop and evaluate a digital toolkit to facilitate employers’ understanding about workplace health screening. Methods: The Test@Work toolkit development included an online survey (STAGE 1: n = 201), stakeholder consultation (STAGE 2: n = 19), expert peer review (STAGE 3: n = 24), and pilot testing (STAGE 4: n = 20). The toolkit includes employer guidance on workplace health promotion, workplace health screening, and confidential opt-in HIV testing with signposting to resources. Pilot testing included assessment of fidelity (delivery and engagement) and implementation qualities (attitudes, resources, practicality, acceptability, usability and cost). Results: STAGE 1: The vast majority of respondents would consider offering general health checks in the workplace that included confidential opt-in HIV testing, and this view was broadly comparable across organisation types (n = 201; public: 87.8%; private: 89.7%; third: 87.1%). STAGES 2 and 3: Stakeholders highlighted essential content considerations: (1) inclusion of the business case for workplace health initiatives, (2) clear pathways to employer responsibilities, and (3) presenting HIV-related information alongside other areas of health. With regards presentation, stakeholders proposed that the toolkit should be concise, with clear signposting and be hosted on a trusted portal. STAGE 4: Employers were satisfied with the toolkit content, usability and utility. The toolkit had high fidelity with regards to delivery and employer engagement. Assessment of implementation qualities showed high usability and practicality, with low perceived burden for completion and acceptable cost implications. Very few resource challenges were reported, and the toolkit was considered to be appropriate for any type of organisation, irrespective of size or resources. Conclusions: Employers perceived the Test@Work toolkit to be useful, meaningful and appropriate for their needs. This digital resource could be used to support employers to engage with health screening and opt-in HIV testing within the context of workplace health promotion.
Determinants of Bone Health Status in a Multi-Ethnic Population in Klang Valley, Malaysia
Authors: Chan Chin Yi , Subramaniam Shaanthana , Mohamed Norazlina , Ima-Nirwana Soelaiman , Muhammad Norliza , Fairus Ahmad , Ng Pei Yuen , Jamil Nor Aini , Abd Aziz Noorazah , Chin Kok-Yong
Published: January 2020

Background and objectives: Studies on osteoporosis risk factors are limited in Malaysia, so this study assesses the factors associated with bone health assessed using dual-energy X-ray absorptiometry (DXA) among Malaysians aged ≥40 years. Subjects and Methods: Data on demography, medical history, dietary and lifestyle practices of 786 Malaysians (51.4% women) aged ≥40 years recruited in Klang Valley were obtained. Their body composition and bone health were determined using DXA. The association between risk factors and bone health status was assessed using binary logistic regression. Results: The prevalence of suboptimal bone health and osteoporosis was higher in women (59.4% and 16.1%) than men (40.8% and 8.4%). Overall, the predictors of suboptimal bone health and osteoporosis among the subjects were increased age and higher fat mass. Lower monthly income was positively associated with osteoporosis. Being menopausal was a risk factor for both suboptimal bone health and osteoporosis in women. Women with no formal education were more likely to get osteoporosis. Being a smoker and Chinese were positively related to suboptimal bone health among men. Meanwhile, predictors of osteoporosis among men were regular alcohol and dairy product consumption, higher fat mass and having a tertiary education. Conclusions: This study calls for immediate and effective interventions for middle-aged and elderly populations with risk factors to halt the progression of bone loss.
Correction: Gu, Q., et al. Regionalization Analysis and Mapping for the Source and Sink of Tourist Flows. ISPRS Int. J. Geo-Inf. 2019, 8, 314
Authors: Gu Qiushi , Zhang Haiping , Chen Min , Chen Chongcheng
Published: January 2020

The authors wish to make the following corrections to their paper [...]
SIRT3 Acts as a Positive Autophagy Regulator to Promote Lipid Mobilization in Adipocytes via Activating AMPK
Authors: Zhang Tian , Liu Jingxin , Tong Qiang , Lin Ligen
Published: January 2020

Obesity is increasing at an alarming rate worldwide, which is characterized by the excessive accumulation of triglycerides in adipocytes. Emerging evidence has demonstrated that macroautophagy and chaperone-mediated autophagy (CMA) regulate lipid mobilization and play a key role in energy balance. Sirtuin 3 (SIRT3) is an NAD+-dependent deacetylase, which is important in regulating macroautophagy and lipid metabolism. It is still unknown whether SIRT3 modulates macroautophagy and CMA in adipocytes. The current study found that macroautophagy was dynamically regulated during 3T3-L1 adipocyte differentiation, which coincided with SIRT3 expression. In mature adipocytes, overexpression of SIRT3 activated macroautophagy, mainly on lipid droplets (LDs), through activating the AMP-activated protein kinase (AMPK)-unc-51-like kinase 1 (ULK1) pathway, which in turn resulting in smaller LD size and reduced lipid accumulation. Moreover, SIRT3 overexpression induced the formation of perilipin-1 (PLN1)-heat shock cognate 71 kDa protein (HSC70)-lysosome-associated membrane protein 2 (LAMP2) complex, to activate CMA and cause the instability of LDs in adipocytes. In summary, we found SIRT3 is a positive regulator of macroautophagy and CMA in adipocytes, which might be a promising therapeutic target for treatment of obesity and its related metabolic dysfunction.
Effect of 15 BMI-Associated Polymorphisms, Reported for Europeans, across Ethnicities and Degrees of Amerindian Ancestry in Mexican Children
Authors: Costa-Urrutia Paula , Abud Carolina , Franco-Trecu Valentina , Colistro Valentina , Rodríguez-Arellano Martha Eunice , Alvarez-Fariña Rafael , Acuña Alonso Víctor , Bertoni Bernardo , Granados Julio
Published: January 2020

In Mexico, the genetic mechanisms underlying childhood obesity are poorly known. We evaluated the effect of loci, known to be associated with childhood body mass index (BMI) in Europeans, in Mexican children from different ethnic groups. We performed linear and logistic analyses of BMI and obesity, respectively, in Mestizos and Amerindians (Seris, Yaquis and Nahuatl speakers) from Northern (n = 369) and Central Mexico (n = 8545). We used linear models to understand the effect of degree of Amerindian ancestry (AMA) and genetic risk score (GRS) on BMI z-score. Northern Mexican Mestizos showed the highest overweight-obesity prevalence (47.4%), followed by Seri (36.2%) and Central Mexican (31.5%) children. Eleven loci (SEC16B/rs543874, OLFM4/rs12429545/rs9568856, FTO/rs9939609, MC4R/rs6567160, GNPDA2/rs13130484, FAIM2/rs7132908, FAM120AOS/rs944990, LMX1B/rs3829849, ADAM23/rs13387838, HOXB5/rs9299) were associated with BMI and seven (SEC16B/rs543874, OLFM4/rs12429545/rs9568856, FTO/rs9939609, MC4R/rs6567160, GNPDA2 rs13130484, LMX1B/rs3829849) were associated with obesity in Central Mexican children. One SNP was associated with obesity in Northern Mexicans and Yaquis (SEC16B/rs543874). We found higher BMI z-score at higher GRS (β = 0.11, p = 0.2 × 10−16) and at lower AMA (β = −0.05, p = 6.8 × 10−7). The GRS interacts with AMA to increase BMI (β = 0.03, p = 6.08 × 10−3). High genetic BMI susceptibility increase the risk of higher BMI, including in Amerindian children.
Recent Advances in Lipopolysaccharide Recognition Systems
Authors: Mazgaeen Lalita , Gurung Prajwal
Published: January 2020

Lipopolysaccharide (LPS), commonly known as endotoxin, is ubiquitous and the most-studied pathogen-associated molecular pattern. A component of Gram-negative bacteria, extracellular LPS is sensed by our immune system via the toll-like receptor (TLR)-4. Given that TLR4 is membrane bound, it recognizes LPS in the extracellular milieu or within endosomes. Whether additional sensors, if any, play a role in LPS recognition within the cytoplasm remained unknown until recently. The last decade has seen an unprecedented unfolding of TLR4-independent LPS sensing pathways. First, transient receptor potential (TRP) channels have been identified as non-TLR membrane-bound sensors of LPS and, second, caspase-4/5 (and caspase-11 in mice) have been established as the cytoplasmic sensors for LPS. Here in this review, we detail the brief history of LPS discovery, followed by the discovery of TLR4, TRP as the membrane-bound sensor, and our current understanding of caspase-4/5/11 as cytoplasmic sensors.
Preclinical Activity of Embryonic Annexin A2-Specific Chimeric Antigen Receptor T Cells Against Ovarian Cancer
Authors: Leong Leonard , Tan Heng Liang , Cua Simeon , Yong Kylie Su Mei , Chen Qingfeng , Choo Andre
Published: January 2020

Chimeric antigen receptors (CARs) have found clinical success in B cell malignancies, but a dearth of potential targets limits their wider clinical application, especially in solid tumours. Here, we describe the development of an anti-annexin A2 CAR, CAR(2448), derived from an antibody found to have activity against epithelial ovarian cancer cell lines. The spacer length of CAR(2448) was optimised based on in vitro cytotoxic activity against ovarian cancer (OC) cell lines via a real-time cytotoxicity assay. The longer spacer CAR(2448)L T cells exhibit significant effector activity, inducing inflammatory cytokine release and cytotoxicity against OC cell lines. Furthermore, CAR(2448)L-BBz T cells induced enhanced survival in an in vivo OC xenograft model and reduced tumour volume by 76.6%. Our preclinical studies of CAR(2448) suggest its potential for the unmet need of novel strategies for the treatment of ovarian cancer.
New Insights into the Biological and Pharmaceutical Properties of Royal Jelly
Authors: Ahmad Saboor , Campos Maria Graça , Fratini Filippo , Altaye Solomon Zewdu , Li Jianke
Published: January 2020

Royal jelly (RJ) is a yellowish-white and acidic secretion of hypopharyngeal and mandibular glands of nurse bees used to feed young worker larvae during the first three days and the entire life of queen bees. RJ is one of the most appreciated and valued natural product which has been mainly used in traditional medicines, health foods, and cosmetics for a long time in different parts of the world. It is also the most studied bee product, aimed at unravelling its bioactivities, such as antimicrobial, antioxidant, anti-aging, immunomodulatory, and general tonic action against laboratory animals, microbial organisms, farm animals, and clinical trials. It is commonly used to supplement various diseases, including cancer, diabetes, cardiovascular, and Alzheimer’s disease. Here, we highlight the recent research advances on the main bioactive compounds of RJ, such as proteins, peptides, fatty acids, and phenolics, for a comprehensive understanding of the biochemistry, biological, and pharmaceutical responses to human health promotion and life benefits. This is potentially important to gain novel insight into the biological and pharmaceutical properties of RJ.
Rho Kinase Activity, Connexin 40, and Atrial Fibrillation: Mechanistic Insights from End-Stage Renal Disease on Dialysis Patients
Authors: Calò Lorenzo A. , Ravarotto Verdiana , Bertoldi Giovanni , Pagnin Elisa , Rossi Barbara , Rigato Matteo , Davis Paul A. , Proietti Riccardo
Published: January 2020

Evidence on cellular/molecular mechanisms leading to atrial fibrillation (AF) are scanty. Increased expression of Rho kinase (ROCK) and myosin-phosphatase-target subunit-1 (MYPT-1), ROCK activity’s marker, were shown in AF patients, which correlated with connexin 40 (Cx40) expression, membrane protein of heart gap junctions, key for rapid action potential’s cell–cell transfer. AF is the most frequent arrhythmia in dialysis patients who present increased MYPT-1 phosphorylation, which correlates with left ventricular (LV) mass. Given ROCK’s established role in cardiovascular–renal remodeling, induction of impaired cell-to-cell coupling/potential conduction promoting AF initiation/perpetuation, we evaluated in dialysis patients with AF, MYPT-1 phosphorylation, Cx40 expression, and their relationships to support their involvement in AF. Mononuclear cells’ MYPT-1 phosphorylation, Cx40 expression, and the ROCK inhibitor fasudil’s effect were assessed in dialysis patients with AF (DPAFs), dialysis patients with sinus rhythm (DPs), and healthy subjects (C) (western blot). M-mode echocardiography assessed LV mass and left atrial systolic volume. DPAF’s phospho-MYPT-1 was increased vs. that of DPs and C (1.57 ± 0.17 d.u. vs. 0.69 ± 0.04 vs. 0.51 ± 0.05 respectively, p < 0.0001). DP’s phospho-MYPT-1 was higher vs. that of C, p = 0.009. DPAF’s Cx40 was higher vs. that of DPs and C (1.23 ± 0.12 vs. 0.74 ± 0.03 vs. 0.69 ± 0.03, p < 0.0001). DPAF’s phospho-MYPT-1 correlated with Cx40 (p < 0.001), left atrial systolic volume (p = 0.013), and LV mass (p = 0.014). In DPAFs, fasudil reduced MYPT-1 phosphorylation (p < 0.01) and Cx40 expression (p = 0.03). These data point toward ROCK and Cx40’s role in the mechanism(s) leading to AF in dialysis patients. Exploration of the ROCK pathway in AF could contribute to AF generation’s mechanistic explanations and likely identify potential pharmacologic targets for translation into treatment.
IMCs Microstructure Evolution Dependence of Mechanical Properties for Ni/Sn/Ni Micro Solder-Joints
Authors: Ren Ning , Fang Heng , Wang Dong , Hou Chenyi , Zhao Yatao , Chen Fan , Tian Ye , Paik Kyung-Wook , Wu Yiping
Published: January 2020

The current miniaturization trend of microelectronic devices drives the size of solder joints to continually scale down. The miniaturized joints considerably increase intermetallic compounds (IMCs) volume fraction to trigger mechanical reliability issues. This study investigated precise relationships between varying IMC volumes and mechanical properties of Ni/Sn(20μm)/Ni micro-joints. A designed method that followed the IMC volume as the only variable was used to prepare micro-joint samples with different IMC volumes. The continuously thickened Ni3Sn4 IMCs exhibited a noticeable morphology evolution from rod-like to chunky shape. The subsequent tensile tests showed unexpected tensile strength responses as increasing Ni3Sn4 volume, which was strongly associated with the Ni3Sn4 morphological evolutions. Fractographic analysis displayed that the ductile fracture dominates the 20%–40% IMC micro-joints, whereas the brittle fracture governs the 40%–80% IMC micro-joints. For the ductile fracture-dominated joints, an abnormal reduction in strength occurred as increasing IMCs volume from 20% to 40%. This is primarily due to severe stress concentrations caused by the transformed long rod-typed morphology of the Ni3Sn4. For the brittle fracture-dominated joints, the strength appeared a monotonous increase as the Ni3Sn4 volume increased. This may be attributed to the increased crack resistance resulting from continuous coarsening of the chunky Ni3Sn4 without any voids. Moreover, the finite element analysis was provided to further understand the joint failure mechanisms.
On Residual Stress Development, Prevention, and Compensation in Metal Additive Manufacturing
Authors: Carpenter Kevin , Tabei Ali
Published: January 2020

One of the most appealing qualities of additive manufacturing (AM) is the ability to produce complex geometries faster than most traditional methods. The trade-off for this advantage is that AM parts are extremely vulnerable to residual stresses (RSs), which may lead to geometrical distortions and quality inspection failures. Additionally, tensile RSs negatively impact the fatigue life and other mechanical performance characteristics of the parts in service. Therefore, in order for AM to cross the borders of prototyping toward a viable manufacturing process, the major challenge of RS development must be addressed. Different AM technologies contain many unique features and parameters, which influence the temperature gradients in the part and lead to development of RSs. The stresses formed in AM parts are typically observed to be compressive in the center of the part and tensile on the top layers. To mitigate these stresses, process parameters must be optimized, which requires exhaustive and costly experimentations. Alternative to experiments, holistic computational frameworks which can capture much of the physics while balancing computational costs are introduced for rapid and inexpensive investigation into development and prevention of RSs in AM. In this review, the focus is on metal additive manufacturing, referred to simply as “AM”, and, after a brief introduction to various AM technologies and thermoelastic mechanics, prior works on sources of RSs in AM are discussed. Furthermore, the state-of-the-art knowledge on RS measurement techniques, the influence of AM process parameters, current modeling approaches, and distortion prevention approaches are reported.
Production of Sustainable Construction Materials Using Agro-Wastes
Authors: Maraveas Chrysanthos
Published: January 2020

The construction sector, in modern times, is faced by a myriad of challenges primarily due to the increase in the urban population and dwindling natural resources that facilitate the production of construction materials. Furthermore, higher awareness on climate change is forcing companies to rethink their strategies in developing more sustainable construction materials. Diverse types of agro-waste ranging from rice husk ash (RHA), sugarcane bagasse ash (SCBA), and bamboo leaves ash (BLA) among others have been identified as potent solutions in the development of sustainable construction materials. In this review paper, six different construction materials, made using agro-waste products, are examined. The materials include brick/masonry elements, green concrete, insulation materials for buildings, reinforcement materials for buildings, particleboards, and bio-based plastics. The main criterion adopted in selecting the materials regards their popularity and wide-scale use in modern construction applications. Additionally, as this research emphasizes identifying alternative approaches to develop sustainable construction materials, the focus is directed toward mainstream materials whose continued use has an adverse impact on the environment. The findings obtained from the review showed that the use of agro-waste to develop sustainable construction materials was effective, as the developed materials adhered to established building standards. Therefore, this indicates that agro-waste materials have the potential to replace conventional construction materials and hence achieve economic, environmental, and social sustainability in the long run.
The Effect of Microstructural Imperfections on Corrosion Fatigue of Additively Manufactured ER70S-6 Alloy Produced by Wire Arc Deposition
Authors: Ron Tomer , Levy Galit Katarivas , Dolev Ohad , Leon Avi , Shirizly Amnon , Aghion Eli
Published: January 2020

This study aims at evaluating the effect of microstructure imperfections on the corrosion fatigue performance of an ER70S-6 alloy produced by wire arc additive manufacturing (WAAM) process, in a 3.5% NaCl solution. For reference, a regular ST-37 alloy with relatively similar chemical composition was considered as a counterpart alloy. This was justified by the fact that the ER70S-6 alloy is usually used for conventional welding of ST-37 steel. The results obtained indicated that while the ST-37 alloy exhibited fatigue strength of 240 MPa in the corrosive solution, the additively manufactured ER70S-6 alloy showed fatigue strength of only 140 MPa. These differences were related to microstructural imperfections that are inherently produced during the WAAM process.
Effects of Thymoquinone on Small-Molecule Metabolites in a Rat Model of Cerebral Ischemia Reperfusion Injury Assessed using MALDI-MSI
Authors: Tian Fang , Liu Runzhe , Fan Chaoxin , Sun Yi , Huang Xi , Nie Zongxiu , Zhao Xin , Pu Xiaoping
Published: January 2020

Thymoquinone is one of the main components present in Nigella sativa seeds and is known to have various biological functions in inflammation, oxidative stress, tumors, aging, and in lowering blood glucose levels. Few studies have focused on its neuroprotective effects and its regulation of small-molecule metabolites during cerebral ischemia reperfusion injury. In this study, transient middle cerebral occlusion (tMCAO) was used to establish the rat model of cerebral ischemia reperfusion injury. We investigated the effects of thymoquinone using matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) in a model of ischemia reperfusion injury to explore the changes in small-molecule metabolites in the brain. We found that that thymoquinone significantly improved neurobehavioral scores, reduced the cerebral infarct area, alleviated brain edema, and increased the number of normal neurons following injury. MALDI-MSI revealed that thymoquinone reduced abnormal accumulations of glucose, citric acid, succinate and potassium ions. Thymoquinone also increased the amount of energy-related molecules such as ADP, AMP, GMP, and creatine, antioxidants such as glutathione, ascorbic acid, and taurine, and other metabolism-related molecules such as glutamate, glutamine, aspartate, N-acetyl-L-aspartate, and sodium ions in damaged areas of the brain following cerebral ischemia reperfusion injury. In summary, based on the neuroprotective effect of thymoquinone on cerebral ischemia reperfusion injury, this study revealed the regulation of thymoquinone on energy metabolism and small-molecule substance metabolism.
Substrate Profiling of the Cobalt Nitrile Hydratase from Rhodococcus rhodochrous ATCC BAA 870
Authors: Mashweu Adelaide R. , Chhiba-Govindjee Varsha P. , Bode Moira L. , Brady Dean
Published: January 2020

The aromatic substrate profile of the cobalt nitrile hydratase from Rhodococcus rhodochrous ATCC BAA 870 was evaluated against a wide range of nitrile containing compounds (>60). To determine the substrate limits of this enzyme, compounds ranging in size from small (90 Da) to large (325 Da) were evaluated. Larger compounds included those with a bi-aryl axis, prepared by the Suzuki coupling reaction, Morita–Baylis–Hillman adducts, heteroatom-linked diarylpyridines prepared by Buchwald–Hartwig cross-coupling reactions and imidazo[1,2-a]pyridines prepared by the Groebke–Blackburn–Bienaymé multicomponent reaction. The enzyme active site was moderately accommodating, accepting almost all of the small aromatic nitriles, the diarylpyridines and most of the bi-aryl compounds and Morita–Baylis–Hillman products but not the Groebke–Blackburn–Bienaymé products. Nitrile conversion was influenced by steric hindrance around the cyano group, the presence of electron donating groups (e.g., methoxy) on the aromatic ring, and the overall size of the compound.
The Application of Reversible Intramolecular Sulfonamide Ligation to Modulate Reactivity in Organometallic Ruthenium(II) Diamine Complexes
Authors: Kemp Samuel A. , Prior Timothy J. , Savoie Huguette , Boyle Ross W. , Murray Benjamin S.
Published: January 2020

Metallation of biomacromolecular species forms the basis for the anticancer activity of many metallodrugs. A major limitation of these compounds is that their reactivity is indiscriminate and can, in principle, occur in healthy tissue as well as cancerous tissue, potentially leading to side effects in vivo. Here we present pH-dependent intramolecular coordination of an arene-tethered sulfonamide functionality in organometallic ruthenium(II) ethylenediamine complexes as a route to controlling the coordination environment about the central metal atom. Through variation of the sulfonamide R group and the length of the tether linking it to the arene ligand the acidity of the sulfonamide NH group, and hence the pH-region over which regulation of metal coordination occurs, can be modulated. Intramolecular sulfonamide ligation controlled the reactivity of complex 4 within the physiologically relevant pH-region, rendering it more reactive towards 5ʹ-GMP in mildly acidic pH-conditions typical of tumour tissue compared to the mildly alkaline pH-conditions typical of healthy tissue. However, the activation of 4 by ring-opening of the chelate was found to be a slow process relative to the timescale of typical cell culture assays and members of this series of complexes were found not to be cytotoxic towards the HT-29 cell line. These complexes provide the basis for the development of analogues of increased potency where intramolecular sulfonamide ligation regulates reactivity and therefore cytotoxicity in a pH-dependent, and potentially, tissue-dependent manner.
Herba houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade
Authors: Cao Ke , Lv Weiqiang , Liu Xuyun , Fan Yingying , Wang Kexin , Feng Zhihui , Liu Jianshu , Zang Weijin , Xing Lianxi , Liu Jiankang
Published: January 2020

Hyperlipidemia is associated with metabolic disorders, but the detailed mechanisms and related interventions remain largely unclear. As a functional food in Asian diets, Herba houttuyniae has been reported to have beneficial effects on health. The present research was to investigate the protective effects of Herba houttuyniae aqueous extract (HAE) on hyperlipidemia-induced liver and heart impairments and its potential mechanisms. Male C57BL/6J mice were administered with 200 or 400 mg/kg/day HAE for 9 days, followed by intraperitoneal injection with 0.5 g/kg poloxamer 407 to induce acute hyperlipidemia. HAE treatment significantly attenuated excessive serum lipids and tissue damage markers, prevented hepatic lipid deposition, improved cardiac remodeling, and ameliorated hepatic and cardiac oxidative stress induced by hyperlipidemia. More importantly, NF-E2 related factor (Nrf2)-mediated antioxidant and peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α)-mediated mitochondrial biogenesis pathways as well as mitochondrial complex activities were downregulated in the hyperlipidemic mouse livers and hearts, which may be attributable to the loss of adenosine monophosphate (AMP)-activated protein kinase (AMPK) activity: all of these changes were reversed by HAE supplementation. Our findings link the AMPK/PGC-1α/Nrf2 cascade to hyperlipidemia-induced liver and heart impairments and demonstrate the protective effect of HAE as an AMPK activator in the prevention of hyperlipidemia-related diseases.
The Influence of Socioeconomic Status on Snacking and Weight among Adolescents: A Scoping Review
Authors: Williamson Victoria , Dilip Abhaya , Dillard Julia , Morgan-Daniel Jane , Lee Alexandra , Cardel Michelle
Published: January 2020

Eating behaviors, including unhealthy snacking or excessive snacking leading to excess calorie consumption, may contribute to obesity among adolescents. Socioeconomic status (SES) also significantly influences eating behaviors, and low SES is associated with increased risk for obesity. However, little is known regarding the relationship between snacking behavior and SES among adolescents and how this may contribute to obesity-related outcomes. The primary objective of this scoping review was to review the literature to assess and characterize the relationship between SES and snacking in adolescents. The secondary objective was to assess weight-related outcomes and their relation to snacking habits. Included articles were published between January 2000 and May 2019; written in English, Portuguese, or Spanish; and focused on adolescents (13–17 years). In total, 14 bibliographic databases were searched, and seven studies met the inclusion criteria. Preliminary evidence from the seven included studies suggests a weak but potential link between SES and snacking. Additionally, these dietary patterns seemed to differ by sex and income type of country. Finally, only three of the included studies addressed weight-related outcomes, but the overall available evidence suggests that snacking does not significantly affect weight-related outcomes. Due to the small number of included studies, results should be interpreted with caution.
Sugar Reduction in Yogurt Products Sold in the UK between 2016 and 2019
Authors: Moore J. Bernadette , Sutton Eiméar H. , Hancock Neil
Published: January 2020

The UK government has called for industry reformulation of foods that contribute most to sugar consumption in children’s diets, including yogurts. The aim of this work was to comprehensively survey yogurt products available in UK supermarkets in 2019 to determine whether sugar contents had been reduced since our baseline survey in 2016. Product information was collected for 893 unique yogurt, fromage frais and dairy dessert products, and nutrient contents were analysed in comparison to those previously examined. Examining all products, there was a highly significant (p < 0.0001) reduction in the median total sugar contents in 2019 compared to those in 2016: median (interquartile range): 10.4 g/100 g (6.6, 13.0) versus 11.9 g/100 (8.8, 13.6). However, notable product turnover was evident; while 60% of the 2019 products surveyed could be matched by brand and name to 2016, 40% were new. In scrutinising paired products closely, only 32% (173 of 539) had reduced sugar contents with a smaller mean difference of −0.65 g/100 g (p < 0.0001), suggesting that the overall median had dropped as a result of higher sugar products being discontinued. Categories showing the most improvements were children’s, drinks and fruit yogurts. Although only 15% of the 2019 products contained ≤5 g/100 g sugars, considered a ‘low-sugar’ product for labelling, this was an improvement over the 9% identified in 2016. Our results yield important insights into current market trends and demonstrate that the median sugar content of UK yogurt products has been reduced by 13% in two years. These data independently evidence modest, but encouraging changes in response to public policy initiatives aimed at preventing childhood obesity.
Mitogen-Activated Protein Kinase Inhibitors and T-Cell-Dependent Immunotherapy in Cancer
Authors: Kumar Sandeep , Principe Daniel R. , Singh Sunil Kumar , Viswakarma Navin , Sondarva Gautam , Rana Basabi , Rana Ajay
Published: January 2020

Mitogen-activated protein kinase (MAPK) signaling networks serve to regulate a wide range of physiologic and cancer-associated cell processes. For instance, a variety of oncogenic mutations often lead to hyperactivation of MAPK signaling, thereby enhancing tumor cell proliferation and disease progression. As such, several components of the MAPK signaling network have been proposed as viable targets for cancer therapy. However, the contributions of MAPK signaling extend well beyond the tumor cells, and several MAPK effectors have been identified as key mediators of the tumor microenvironment (TME), particularly with respect to the local immune infiltrate. In fact, a blockade of various MAPK signals has been suggested to fundamentally alter the interaction between tumor cells and T lymphocytes and have been suggested a potential adjuvant to immune checkpoint inhibition in the clinic. Therefore, in this review article, we discuss the various mechanisms through which MAPK family members contribute to T-cell biology, as well as circumstances in which MAPK inhibition may potentiate or limit cancer immunotherapy.
Effects of Endophytic Bacillus Subtilis and Salicylic Acid on Postharvest Diseases (Phytophthora infestans, Fusarium oxysporum) Development in Stored Potato Tubers
Authors: Lastochkina Oksana , Baymiev Andrey , Shayahmetova Aysylu , Garshina Darya , Koryakov Igor , Shpirnaya Irina , Pusenkova Liudmila , Mardanshin Il’dar , Kasnak Cemal , Palamutoglu Recep
Published: January 2020

Postharvest diseases of potato lead to significant food and economic losses worldwide. The exogenous application of eco-friendly methods plays an important role in the control of postharvest decay. In this work the effects of endophytic bacteria B. subtilis (10-4, 26D) were studied in the context of two application parameters: concentration, with a range between 103–108 CFU/mL tested, and synergistic effects of the signal molecule salicylic acid (SA) (0.05 mM) on potato tubers’ resistance to Phytophthora infestans and Fusarium oxysporum during storage. The experiments were carried out on hydroponically grown potato (Solanum tuberosum L.) mini-tubers. This study demonstrates the suppressive effect of B. subtilis (10-4, 26D) on diseases of potato during storage and reveals that this effect happens in a dose-dependent manner, both individually and in combination with SA. The most effective concentrations of B. subtilis for suppression of both Ph. infestans and F. oxysporum are 108 CFU/mL (10-4 and 26D), 107 CFU/mL (10-4 + SA) and 106 CFU/mL (26D + SA). The ability of B. subtilis (10-4, 26D) to effectively penetrate and colonize the internal tubers’ tissues when applied immediately prior to storage, and the ability of SA to accelerate these processes, have been proven. B. subtilis (10-4, 26D), individually and in compositions with SA, increased ascorbic acid content and decreased pathogen-induced proline accumulation and lipid peroxidation in tubers. This indicates a protective effect conferred to cells against reactive oxygen and an extension of aging processes, manifested by a prolonged shelf life and extended preservation of fresh appearance.
Fabrication and Optimization of the Thermo-Sensitive Hydrogel Carboxymethyl Cellulose/Poly(N-isopropylacrylamide-co-acrylic acid) for U(VI) Removal from Aqueous Solution
Authors: Tan Juan , Xie Shuibo , Wang Guohua , Yu Chuck Wah , Zeng Taotao , Cai Pingli , Huang Huayong
Published: January 2020

In this work, the thermo-sensitive materials N-isopropylacrylamide (NIPAM) and acrylic acid (AA) were crosslinked with carboxymethyl cellulose (CMC) (CMC/P (NIPAM-co-AA)) via a free radical polymerization method for the removal of U(VI) from aqueous solution. The L16 (45) orthogonal experiments were designed for the optimization of the synthesis condition. The chemical structures of the crosslinking hydrogel were confirmed by FTIR spectroscopy. The microstructural analyses were conducted though scanning electron microscopy (SEM) to show the pore structure of the hydrogel. The adsorption performance of the CMC/P (NIPAM-co-AA) hydrogel for the uptake of U(VI) from simulated wastewater was also investigated. The adsorption reached equilibrium within 1 h. Under the reaction of pH = 6 and a temperature of 298 K, an initial concentration of U(VI) of 5 mg·L−1, and 10 mg of the CMC/P(NIPAM-co-AA) hydrogel, the maximum adsorption capacity was 14.69 mg g−1. The kinetics fitted perfectly with the pseudo-second-order model, and the isotherms for the composite hydrogel adsorption of U(VI) was in accordance with the Langmuir model. The chemical modification confirmed that the acylamino group played an important role in uranium adsorption. The desorption and reusability study revealed that the resolution rate was still available at approximately 77.74% after five alternate heating cycles at 20 and 50 °C of adsorption-desorption.
Interrogating Gender in Sikh Tradition and Practice
Authors: Bains Satwinder Kaur
Published: January 2020

In contemporary Sikh society, what we consider religious is constantly being challenged, but for Sikhs, what remain constant are Sikhi’s sacred texts—they continue to be the paramount teacher and guide. Within this consistency, I ask the question: how can Sikh feminist ideas of representation and identity find expression in response to our understanding/practice of our faith, our institutions, and of the everyday Sikh symbols? This paper critically examines the gendered nature of the Guru Granth, practices within the gurdwaras, and focuses on a part of the Rahit Maryada (Code of Conduct) as an area of exploration in the understanding of the everyday ascribed five symbols of Sikhi (punj kakar) through a feminist lens. I undertake this in order to gain a gendered appreciation of how the scriptures, religious institutions, and the articles of faith resonate with the feminine.
Measuring Surface Moisture on a Sandy Beach based on Corrected Intensity Data of a Mobile Terrestrial LiDAR
Authors: Jin Junling , De Sloover Lars , Verbeurgt Jeffrey , Stal Cornelis , Deruyter Greet , Montreuil Anne-Lise , De Maeyer Philippe , De Wulf Alain
Published: January 2020

Surface moisture plays a key role in limiting the aeolian transport on sandy beaches. However, the existing measurement techniques cannot adequately characterize the spatial and temporal distribution of the beach surface moisture. In this study, a mobile terrestrial LiDAR (MTL) is demonstrated as a promising method to detect the beach surface moisture using a phase-based Z&F/Leica HDS6100 laser scanner mounted on an all-terrain vehicle. Firstly, two sets of indoor calibration experiments were conducted so as to comprehensively investigate the effect of distance, incidence angle and sand moisture contents on the backscattered intensity by means of sand samples with an average grain diameter of 0.12 mm. A moisture estimation model was developed which eliminated the effects of the incidence angle and distance (it only relates to the target surface reflectance). The experimental results reveal both the distance and incidence angle influencing the backscattered intensity of the sand samples. The standard error of the moisture model amounts to 2.0% moisture, which is considerably lower than the results of the photographic method. Moreover, a field measurement was conducted using the MTL system on a sandy beach in Belgium. The accuracy and robustness of the beach surface moisture derived from the MTL data was evaluated. The results show that the MTL is a highly suitable technique to accurately and robustly measure the surface moisture variations on a sandy beach with an ultra-high spatial resolution (centimeter-level) in a short time span (12 × 200 m per minute).
Successive Interference Cancellation Based Throughput Optimization for Multi-Hop Wireless Rechargeable Sensor Networks
Authors: Zhang Peng , Ding Xu , Xu Juan , Wang Jing , Shi Lei
Published: January 2020

Wireless Sensor Networks are constrained by low channel utilization and limited battery capacity, so they are widely regarded as the mainly performance bottlenecks. In this paper, in order to improve channel utilization and prolong network lifetime, we investigate the cooperation of multi-hop Wireless Rechargeable Sensor Networks (WRSNs) with Successive Interference Cancellation (SIC) technology. In WRSNs, since the flow rate of each node is unknown, the power of the nodes is not constant. However, SIC will not work if the signal power levels at receive node cannot be sorted. To solve this issue, we first construct a minimum energy routing and unify the transmit rate to determine the transmit power. We can also obtain the time scheduling scheme after determining the routing and power. Next, we formulate an optimization problem, with the objective of maximizing the mobile charger’s vacation time over the rechargeable cycle. Finally, we provide a near-optimal solution and prove its feasible performance. Simulation results present that SIC can achieve the better upper boundary on throughput (compared to inference avoidance increasing about 180–450%) and no extra transmit and receive energy consumption in the multi-hop WRSNs.
Enhancing the Sensor Node Localization Algorithm Based on Improved DV-Hop and DE Algorithms in Wireless Sensor Networks
Authors: Han Dezhi , Yu Yunping , Li Kuan-Ching , de Mello Rodrigo Fernandes
Published: January 2020

The Distance Vector-Hop (DV-Hop) algorithm is the most well-known range-free localization algorithm based on the distance vector routing protocol in wireless sensor networks; however, it is widely known that its localization accuracy is limited. In this paper, DEIDV-Hop is proposed, an enhanced wireless sensor node localization algorithm based on the differential evolution (DE) and improved DV-Hop algorithms, which improves the problem of potential error about average distance per hop. Introduced into the random individuals of mutation operation that increase the diversity of the population, random mutation is infused to enhance the search stagnation and premature convergence of the DE algorithm. On the basis of the generated individual, the social learning part of the Particle Swarm (PSO) algorithm is embedded into the crossover operation that accelerates the convergence speed as well as improves the optimization result of the algorithm. The improved DE algorithm is applied to obtain the global optimal solution corresponding to the estimated location of the unknown node. Among the four different network environments, the simulation results show that the proposed algorithm has smaller localization errors and more excellent stability than previous ones. Still, it is promising for application scenarios with higher localization accuracy and stability requirements.