bluuebunny/crossref_metadata_2025 huggingface dataset:
“We are taking every precaution to do our part…”: a comparative analysis of nursing, palliative and hospice care facilities’ websites during the COVID-19 pandemic
Authors: Ölcer Sabahat , Idris¹ Mohamed , Yilmaz-Aslan Yüce , Brzoska Patrick
Published: June 2021

Background

The COVID-19 pandemic has a significant impact on health care processes. Precautions such as restrictions imposed on visitors and social distancing have led to multiple challenges, particularly in terms of communication. Using the case of nursing, palliative and hospice care facilities in Germany and drawing on agenda-setting theory, the present study aims to shed light on how health care facilities use their websites to inform (potential) health care users about changes in regulations, new protective measures implemented and about recommendations in the context of the COVID-19 pandemic.

Methods

The websites of nursing, palliative and hospice care facilities in Germany were examined using qualitative document analysis and qualitative content analysis. A total of 138 websites was analysed in the study. The data gathered includes all information about COVID-19 on these websites published from the beginning of March until August 15, 2020.

Results

Facilities show similarities in adhering to the measures taken by the authorities to restrict the spread of SARS-CoV-2 and to protect vulnerable patients and employees. All facilities urged the public to avoid paying visits to patients in facilities unless there was an emergency; however, visiting procedures in practice varied by types of facilities. For optimal communication, telephone and especially video calls were the options preferred by health care providers and health care users. Facilities made great efforts to prevent emotional stress and to strengthen resilience among all stakeholders. Transparency was adopted by many facilities in order to build the public’s trust.

Conclusions

The agenda of health care facilities has been seriously affected by the COVID-19 pandemic. The study sheds light on the strategies developed by facilities, their efforts to increase emotional resilience among health care staff and health care users, the ethical guidelines they have adopted regarding privacy policies as well as how these themes are communicated via the facilities’ websites. The results can inform other health care facilities about how websites can be used as essential communication tools in times of public health crises.
A novel theory of Asian elephant high-frequency squeak production
Authors: Beeck Veronika C. , Heilmann Gunnar , Kerscher Michael , Stoeger Angela S.
Published: June 2021

Background

Anatomical and cognitive adaptations to overcome morpho-mechanical limitations of laryngeal sound production, where body size and the related vocal apparatus dimensions determine the fundamental frequency, increase vocal diversity across taxa. Elephants flexibly use laryngeal and trunk-based vocalizations to form a repertoire ranging from infrasonic rumbles to higher-pitched trumpets. Moreover, they are among the few evolutionarily distantly related animals (humans, pinnipeds, cetaceans, birds) capable of imitating species-atypical sounds. Yet, their vocal plasticity has so far not been related to functions within their natural communicative system, in part because not all call types have been systematically studied. Here, we reveal how Asian elephants (Elephas maximus)produce species-specific squeaks (F0 300–2300 Hz) by using acoustic camera recordings to visualize sound emission and examining this alongside acoustic, behavioral, and morphological data across seven captive groups.

Results

We found that squeaks were emitted through the closed mouth in synchrony with cheek depression and retraction of the labial angles. The simultaneous emission of squeaks with nasal snorts (biphonation) in one individual confirmed that squeak production was independent of nasal passage involvement and this implicated oral sound production. The squeaks’ spectral structure is incongruent with laryngeal sound production and aerodynamic whistles, pointing to tissue vibration as the sound source. Anatomical considerations suggest that the longitudinal closed lips function as the vibrators. Acoustic and temporal parameters exhibit high intra- and inter-individual variability that enables individual but no call-subtype classification. Only 19 of 56 study subjects were recorded to squeak, mostly during alarming contexts and social arousal but some also on command.

Conclusion

Our results strongly suggest that Asian elephants force air from the small oral cavity through the tensed lips, inducing self-sustained lip vibration. Besides human brass players, lip buzzing is not described elsewhere in the animal kingdom. Given the complexity of the proposed mechanism, the surprising absence of squeaking in most of the unrelated subjects and the indication for volitional control, we hypothesize that squeak production involves social learning. Our study offers new insights into how vocal and cognitive flexibility enables mammals to overcome size-related limitations of laryngeal sound production. This flexibility enables Asian elephants to exploit a frequency range spanning seven octaves within their communicative system.
Epigenetic landscapes of intracranial aneurysm risk haplotypes implicate enhancer function of endothelial cells and fibroblasts in dysregulated gene expression
Authors: Poppenberg Kerry E. , Zebraski Haley R. , Avasthi Naval , Waqas Muhammad , Siddiqui Adnan H. , Jarvis James N. , Tutino Vincent M.
Published: June 2021

Background

Genome-wide association studies have identified many single nucleotide polymorphisms (SNPs) associated with increased risk for intracranial aneurysm (IA). However, how such variants affect gene expression within IA is poorly understood. We used publicly-available ChIP-Seq data to study chromatin landscapes surrounding risk loci to determine whether IA-associated SNPs affect functional elements that regulate gene expression in cell types comprising IA tissue.

Methods

We mapped 16 significant IA-associated SNPs to linkage disequilibrium (LD) blocks within human genome. Using ChIP-Seq data, we examined these regions for presence of H3K4me1, H3K27ac, and H3K9ac histone marks (typically associated with latent/active enhancers). This analysis was conducted in several cell types that are present in IA tissue (endothelial cells, smooth muscle cells, fibroblasts, macrophages, monocytes, neutrophils, T cells, B cells, NK cells). In cell types with significant histone enrichment, we used HiC data to investigate topologically associated domains (TADs) encompassing the LD blocks to identify genes that may be affected by IA-associated variants. Bioinformatics were performed to determine the biological significance of these genes. Genes within HiC-defined TADs were also compared to differentially expressed genes from RNA-seq/microarray studies of IA tissues.

Results

We found that endothelial cells and fibroblasts, rather than smooth muscle or immune cells, have significant enrichment for enhancer marks on IA risk haplotypes (p < 0.05). Bioinformatics demonstrated that genes within TADs subsuming these regions are associated with structural extracellular matrix components and enzymatic activity. The majority of histone marked TADs (83% fibroblasts [IMR90], 77% HUVEC) encompassed at least one differentially expressed gene from IA tissue studies.

Conclusions

These findings provide evidence that genetic variants associated with IA risk act on endothelial cells and fibroblasts. There is strong circumstantial evidence that this may be mediated through altered enhancer function, as genes in TADs encompassing enhancer marks have also been shown to be differentially expressed in IA tissue. These genes are largely related to organization and regulation of the extracellular matrix. This study builds upon our previous (Poppenberg et al., BMC Med Genomics, 2019) by including a more diverse set of data from additional cell types and by identifying potential affected genes (i.e. those in TADs).
Metabolome analysis reveals a diversity of cancer tissues in advanced epithelial ovarian cancer
Authors: Yoshida Kosuke , Yoshikawa Nobuhisa , Kitami Kazuhisa , Tamauchi Satoshi , Ikeda Yoshiki , Yokoi Akira , Nishino Kimihiro , Niimi Kaoru , Kajiyama Hiroaki
Published: June 2021

Background

Epithelial ovarian cancer remains one of the leading causes of cancer deaths among women worldwide, and advanced epithelial ovarian cancer frequently metastasizes to the omentum. The characteristics of metastatic cancer may differ from those of primary ovarian cancer and reflect the unique omental microenvironment. This study investigated metabolomic differences in epithelial ovarian cancers.

Methods

Patients with advanced epithelial ovarian cancer were eligible for this study. Five patients underwent surgery and resection of paired primary ovarian and omental metastatic cancer at Nagoya University. Metabolome analysis was performed in these paired cancer and metastatic cancer tissues through a facility service (C-SCOPE) at Human Metabolome Technologies, Inc. The concentrations of 116 compounds were measured by CE-TOFMS and CE-QqQMS, and 30 metabolic parameters were calculated. For statistical analyses, Welch’s t-test was used for comparisons between two independent groups.

Results

Metabolite profiles were all different, which reflects diversity among these cancer tissues. Of the measured compounds, urea was the only metabolite that was significantly decreased in omental metastatic cancers compared with the primary cancers (p = 0.031). Moreover, in omental metastatic cancers, the pentose phosphate pathway was more dominant than glycolysis. Furthermore, in some cases, lactic acids in omental metastatic cancers were markedly decreased compared with primary cancers. With regard to histological subtype, the total levels of amino acids, especially the percentage of glutamine, were significantly enriched in serous carcinomas compared with nonserous carcinomas (p = 0.004 and p = 0.001). Moreover, the reduced forms of glutathione and polyamines were also more abundant in serous carcinomas than in nonserous carcinomas (p = 0.025 and 0.048).

Conclusions

The metabolite profiles differed depending on tumor location and histological subtype. Metabolome analysis may be a useful tool for identifying cancer diagnostic and prognostic markers.
Malaria epidemiology and anti-malarial drug efficacy in Guinea: a review of clinical and molecular studies
Authors: Cherif Mahamoud Sama , Dahal Prabin , Beavogui Abdoul Habib , Delamou Alexandre , Lama Eugene Kaman , Camara Alioune , Diallo Mamadou Pathe
Published: June 2021

Malaria is one of the leading causes of mortality and morbidity in Guinea. The entire country is considered at risk of the disease. Transmission occurs all year round with peaks occurring from July through October withPlasmodium falciparumas the primary parasite species. Chloroquine (CQ) was the first-line drug against uncomplicatedP.falciparumin Guinea until 2005, prior to the adoption of artemisinin-based combination therapy (ACT). In this review, data on therapeutic efficacy of CQ and artemisinin-based combinations reported in published literature is summarized. Against CQ, a failure rate of 27% (12/44) was reported in a study in 1992; a median failure rate of 15.6% [range: 7.7–28.3; 8 studies] was observed during 1996–2001, and 81% (17/21) of the patients failed to clear parasitaemia in a study conducted in 2007. For artemisinin-based combinations, three published studies were identified (1495 patients; 2004–2016); all three studies demonstrated day 28 polymerase chain reaction corrected efficacy > 95%. One study characterizedkelch-13mutations (389 tested; samples collected in 2016) with no evidence of mutations currently known to be associated with artemisinin resistance. The impact of the ongoing COVID-19 pandemic and widespread usage of counterfeit medicines are immediate challenges to malaria control activities in Guinea.
Correction to: M2 macrophage-derived exosomal microRNAs inhibit cell migration and invasion in gliomas through PI3K/AKT/mTOR signaling pathway
Authors: Yao Jie , Wang Zefen , Cheng Yong , Ma Chao , Zhong Yahua , Xiao Yilei , Gao Xu , Li Zhiqiang
Published: June 2021

An amendment to this paper has been published and can be accessed via the original article.
Manifestations of HbSE sickle cell disease: a systematic review
Authors: Khamees Ibrahim , Ata Fateen , Choudry Hassan , Soliman Ashraf T. , De Sanctis Vincenzo , Yassin Mohamed A.
Published: June 2021

Background

Sickle cell disease (SCD) is commonly encountered in Africa and Middle Eastern countries. The causative mutation in the gene encoding the hemoglobin subunit β (HBB) leads to various genotypic variants of the disease. This results in varied phenotypes, with a spectrum of complications, from benign to fatal. Hemoglobin SS (HBSS) genotype is associated with most of these complications; hence, it is a severe form of SCD. On the other hand, rare genotypes such as hemoglobin SE (HBSE) are considered benign. There is limited literature about the clinical manifestations and characteristics of patients with HBSE. We pooled all available data describing the phenotypic manifestations of HBSE heterozygote worldwide to perform a systematic review.

Methods

We performed a systematic review according to PRISMA guidelines using PubMed, SCOPUS, and Google Scholar databases. Two independent reviewers (FA and IK) evaluated studies for eligibility and extracted data. We synthesized data on demographics, manifestations, and management of HBSE disease. PROSPERO Registration Number: CRD42021229877.

Results

We found 68 HBSE patients reported in the literature. 24 cases were extracted from case reports whereas 44 cases from case series and retrospective studies. Turkey reported the highest number of patients (n = 22). 32 (47%) of the patients were males. The mean age was 20.9 ± 18.26 years. The mean HBS and HBE percentages were 61.1% ± 7.25% and 32.3% ± 5.06%, respectively, whereas the mean hemoglobin was 11.64 ± 1.73 g/dl. Reported manifestations of HBSE disease included acute vaso-occlusive pain crisis (n = 22, 32.3%), splenomegaly (n = 11, 16.1%), hemolytic anemia (n = 10, 14.7%), infections (n = 8. 11.7%), bone infarction (n = 4, 5.8%), gallstones (n = 3, 4.4%), venous thromboembolism (VTE) (n = 2, 2.9%) and stroke (n = 2, 2.9%), and hematuria (n = 2, 2.9%). Death due to HBSE complications was reported in three patients.

Conclusion

HBSE is a rare genotypic variant of SCD. It has been considered a benign form; however, there are multiple reports of severe complications. Severe complications observed in HBSE disease include vaso-occlusive crisis, acute chest syndrome, stroke, bone marrow embolism, and death.
Intimate partner violence against adolescents and young women in sub-Saharan Africa: who is most vulnerable?
Authors: Wado Yohannes Dibaba , Mutua Martin K. , Mohiddin Abdu , Ijadunola Macellina Y. , Faye Cheikh , Coll Carolina V. N. , Barros Aluisio J. D. , Kabiru Caroline W.
Published: June 2021

Background

Intimate partner violence (IPV) is a global public health and human rights issue that affects millions of women and girls. While disaggregated national statistics are crucial to assess inequalities, little evidence exists on inequalities in exposure to violence against adolescents and young women (AYW). The aim of this study was to determine inequalities in physical or sexual IPV against AYW and beliefs about gender based violence (GBV) in sub-Saharan Africa (SSA).

Methods

We used data from the most recent Demographic and Health Surveys (DHS) conducted in 27 countries in SSA. Only data from surveys conducted after 2010 were included. Our analysis focused on married or cohabiting AYW aged 15–24 years and compared inequalities in physical or sexual IPV by place of residence, education and wealth. We also examined IPV variations by AYW’s beliefs about GBV and the association of country characteristics such as gender inequality with IPV prevalence.

Results

The proportion of AYW reporting IPV in the year before the survey ranged from 6.5% in Comoros to 43.3% in Gabon, with a median of 25.2%. Overall, reported IPV levels were higher in countries in the Central Africa region than other sub-regions. Although the prevalence of IPV varied by place of residence, education and wealth, there was no clear pattern of inequalities. In many countries with high prevalence of IPV, a higher proportion of AYW from rural areas, with lower education and from the poorest wealth quintile reported IPV. In almost all countries, a greater proportion of AYW who approved wife beating for any reason reported IPV compared to their counterparts who disapproved wife beating. Reporting of IPV was weakly correlated with the Gender Inequality Index and other societal level variables but was moderately positively correlated with adult alcohol consumption (r = 0.48) and negative attitudes towards GBV (r = 0.38).

Conclusion

IPV is pervasive among AYW, with substantial variation across and within countries reflecting the role of contextual and structural factors in shaping the vulnerability to IPV. The lack of consistent patterns of inequalities by the stratifiers within countries shows that IPV against women and girls cuts across socio-economic boundaries suggesting the need for comprehensive and multi-sectoral approaches to preventing and responding to IPV.
Are rhetorical commitments to adolescents reflected in planning documents? An exploratory content analysis of adolescent sexual and reproductive health in Global Financing Facility country plans
Authors: George Asha S. , Jacobs Tanya , Kinney Mary V. , Haakenstad Annie , Singh Neha S. , Rasanathan Kumanan , Chopra Mickey
Published: June 2021

Background

The Global Financing Facility (GFF) offers an opportunity to close the financing gap that holds back gains in women, children’s and adolescent health. However, very little work exists examining GFF practice, particularly for adolescent health. As momentum builds for the GFF, we examine initial GFF planning documents to inform future national and multi-lateral efforts to advance adolescent sexual and reproductive health.

Methods

We undertook a content analysis of the first 11 GFF Investment Cases and Project Appraisal Documents available on the GFF website. The countries involved include Bangladesh, Cameroon, Democratic Republic of Congo, Ethiopia, Guatemala, Kenya, Liberia, Mozambique, Nigeria, Tanzania and Uganda.

Results

While several country documents signal understanding and investment in adolescents as a strategic area, this is not consistent across all countries, nor between Investment Cases and Project Appraisal Documents. In both types of documents commitments weaken as one moves from programming content to indicators to investment. Important contributions include how teenage pregnancy is a universal concern, how adolescent and youth friendly health services and school-based programs are supported in several country documents, how gender is noted as a key social determinant critical for mainstreaming across the health system, alongside the importance of multi-sectoral collaboration, and the acknowledgement of adolescent rights. Weaknesses include the lack of comprehensive analysis of adolescent health needs, inconsistent investments in adolescent friendly health services and school based programs, missed opportunities in not supporting multi-component and multi-level initiatives to change gender norms involving adolescent boys in addition to adolescent girls, and neglect of governance approaches to broker effective multi-sectoral collaboration, community engagement and adolescent involvement.

Conclusion

There are important examples of how the GFF supports adolescents and their sexual and reproductive health. However, more can be done. While building on service delivery approaches more consistently, it must also fund initiatives that address the main social and systems drivers of adolescent health. This requires capacity building for the technical aspects of adolescent health, but also engaging politically to ensure that the right actors are convened to prioritize adolescent health in country plans and to ensure accountability in the GFF process itself.
Inequalities in early marriage, childbearing and sexual debut among adolescents in sub-Saharan Africa
Authors: Melesse Dessalegn Y. , Cane Réka M. , Mangombe Aveneni , Ijadunola Macellina Y. , Manu Adom , Bamgboye Eniola , Mohiddin Abdu , Kananura Rornald M. , Akwara Elsie , du Plessis Elsabé , Wado Yohannes D. , Mutua Martin K. , Mekonnen Wubegzier , Faye Cheikh M. , Neal Sarah , Boerma Ties
Published: June 2021

Background

Adolescent sexual and reproductive health (ASRH) is a major public health concern in sub-Saharan Africa (SSA). However, inequalities in ASRH have received less attention than many other public health priority areas, in part due to limited data. In this study, we examine inequalities in key ASRH indicators.

Methods

We analyzed national household surveys from 37 countries in SSA, conducted during 1990–2018, to examine trends and inequalities in adolescent behaviors related to early marriage, childbearing and sexual debut among adolescents using data from respondents 15–24 years. Survival analyses were conducted on each survey to obtain estimates for the ASRH indicators. Multilevel linear regression modelling was used to obtain estimates for 2000 and 2015 in four subregions of SSA for all indicators, disaggregated by sex, age, household wealth, urban–rural residence and educational status (primary or less versus secondary or higher education).

Results

In 2015, 28% of adolescent girls in SSA were married before age 18, declined at an average annual rate of 1.5% during 2000–2015, while 47% of girls gave birth before age 20, declining at 0.6% per year. Child marriage was rare for boys (2.5%). About 54% and 43% of girls and boys, respectively, had their sexual debut before 18. The declines were greater for the indicators of early adolescence (10–14 years). Large differences in marriage and childbearing were observed between adolescent girls from rural versus urban areas and the poorest versus richest households, with much greater inequalities observed in West and Central Africa where the prevalence was highest. The urban–rural and wealth-related inequalities remained stagnant or widened during 2000–2015, as the decline was relatively slower among rural and the poorest compared to urban and the richest girls. The prevalence of the ASRH indicators did not decline or increase in either education categories.

Conclusion

Early marriage, childbearing and sexual debut declined in SSA but the 2015 levels were still high, especially in Central and West Africa, and inequalities persisted or became larger. In particular, rural, less educated and poorest adolescent girls continued to face higher ASRH risks and vulnerabilities. Greater attention to disparities in ASRH is needed for better targeting of interventions and monitoring of progress.
The EOS 3D imaging system reliably measures posterior tibial slope
Authors: Hecker Andreas , Lerch Till D. , Egli Rainer J. , Liechti Emanuel F. , Klenke Frank M.
Published: June 2021

Background

One of the values determined during the assessment of knee issues is the posterior tibial slope (PTS). A new option for measuring the PTS is the EOS 3D imaging system, which provides anteroposterior (AP) and lateral long leg radiographs (LLRs) using less radiation than a conventional LLR. We investigated the reliability of the EOS 3D imaging system with respect to PTS measurements.

Methods

We retrospectively searched our radiological database for patients who underwent an EOS scan and a computed tomography (CT) scan of their lower extremities between January and December 2019. Fifty-six knees were included in the study. Medial and lateral PTSs were determined using both modalities. A radiologist and an orthopaedic surgeon each performed all measurements twice and the intraclass correlation (ICC) was calculated to assess inter- and intrarater reliability. The Student t test and Pearson correlation were used to compare the results of both imaging modalities.

Results

The mean medial PTS was 8.5° (95% confidence interval [CI], 8.1–8.9°) for the EOS system and 7.7° (95% CI, 7.3–8.1°) for CT, and the lateral PTS was 7.4° (95% CI, 6.9–7.9°) for the EOS system, and 7.0° (95% CI, 6.5–7.4°) for CT. Interrater reliability (ICC) with respect to medial and lateral PTSs measured on the EOS (0.880, 0.765) and CT (0.884, 0.887) images was excellent. The intrarater reliability of reader 1 (ICC range, 0.889–0.986) and reader 2 (ICC range, 0.868–0.980) with respect to the same measurements was excellent.

Conclusion

The PTS measurements from the EOS 3D imaging system are as reliable and reproducible as those from CT, the current gold standard method. We recommend using this system if possible, because it acquires more information (sagittal plane) in a scan than a conventional LLR, while exposing the patient to less radiation.

Level of evidence

Level III, Retrospective cohort study
A community-based service enhancement model of training and employing Ear Health Facilitators to address the crisis in ear and hearing health of Aboriginal children in the Northern Territory, the Hearing for Learning Initiative (the HfLI): study protocol for a stepped-wedge cluster randomised trial
Authors: Kong Kelvin , Cass Alan , Leach Amanda Jane , Morris Peter Stanley , Kimber Amy , Su Jiunn-Yih , Oguoma Victor Maduabuchi
Published: June 2021

Background

Almost all Aboriginal children in remote communities have persistent bilateral otitis media affecting hearing and learning throughout early childhood and school years, with consequences for social and educational outcomes, and later employment opportunities. Current primary health care and specialist services do not have the resources to meet the complex needs of these children.

Method/design

This stepped-wedge cluster randomised trial will allocate 18 communities to one of five 6-monthly intervention start dates. Stratification will be by region and population size. The intervention (Hearing for Learning Initiative, HfLI) consists of six 20-h weeks of training (delivered over 3 months) that includes Certificate II in Aboriginal Primary Health Care (3 modules) and competencies in ear and hearing data collection (otoscopy, tympanometry and hearScreen), plus 3 weeks of assisted integration into the health service, then part-time employment as Ear Health Facilitators to the end of the trial. Unblinding will occur 6 months prior to each allocated start date, to allow Community Reference Groups to be involved in co-design of the HfLI implementation in their community. Relevant health service data will be extracted 6-monthly from all 18 communities. The primary outcome is the difference in proportion of children (0 to 16 years of age) who have at least one ear assessment (diagnosis) documented in their medical record within each 6-month period, compared to control periods (no HfLI). Secondary outcomes include data on sustainability, adherence to evidence-based clinical guidelines for otitis media, including follow-up and specialist referrals, and school attendance. Structured interviews with staff working in health and education services, Ear Health Trainees, Ear Health Facilitators and families will assess process outcomes and the HfLI broader impact.

Discussion

The impact of training and employment of Ear Health Facilitators on service enhancement will inform the health, education and employment sectors about effectiveness of skills and job creation that empowers community members to contribute to addressing issues of local importance, in this instance ear and hearing health of children.

Trial registration

ClinicalTrials.gov NCT03916029. Registered on 16 April 2019.
Effectiveness of a digitally supported care management programme to reduce unmet needs of family caregivers of people with dementia: study protocol for a cluster randomised controlled trial (GAIN)
Authors: Klein Olga A. , Boekholt Melanie , Afrin Dilshad , Dornquast Christina , Dreier-Wolfgramm Adina , Keller Armin , Michalowsky Bernhard , Zwingmann Ina , Teipel Stefan , Thyrian Jochen René , Kilimann Ingo , Hoffmann Wolfgang
Published: June 2021

Background

Up to two-thirds of dementia care is provided by family caregivers who often experience high burden, little support and adverse health outcomes. Enabling and supporting family caregivers to provide care at home prevents early institutionalisation of the person with dementia and alleviates the economic burden of dementia in the long term. General practitioners (GPs), as the first point of contact, have a key role in identifying and managing burden and care needs of family caregivers. However, in routine care, this opportunity is often limited by time constraints and even if caregiver needs are recognised, detailed information about regionally available support and advice on healthcare services is often lacking.

Methods

This is a cluster randomised, controlled trial investigating the clinical use and cost-effectiveness of a digitally supported care management programme for caregivers of people with dementia (PwD). Five hundred family caregivers will be randomised at GP offices, specialist practices and memory clinics, with about n=250 participants per arm. Participants are eligible if they are the primary family caregiver of a PwD, are at least 18 years of age and provide informed consent. Participants in the intervention group will receive an individualised care management plan, which will be carried out by qualified study nurses in collaboration with the treating GP. All participants will receive a baseline assessment and a 6-months follow-up assessment. Participants in the wait-list control group will receive usual care. Starting at the 6 months’ follow-up, the former controls will also receive an individualised management plan. Primary outcomes are the number of unmet needs (incl. the Camberwell Assessment of Need for the Elderly, CANE) and health-related quality of life (EQ-5D-5L) at 6 months. Secondary outcomes include caregiver burden (Zarit Burden Interview, ZBI), social support (Lubben Social Network Scale, LSNS), the use of medical and non-medical services (Questionnaire for the Use of Medical and Non-Medical Services, FIMA) and resource utilisation (Resource Utilisation in Dementia, RUD). The primary analysis will be based on intention-to-treat. Between- and within-group analyses and a cost-effectiveness analysis will be conducted to estimate the effect of the tablet PC-based care management programme. This trial is funded by the German Federal Joint Committee (G-BA) Innovation Fund.

Discussion

The findings of this trial will be useful in informing and improving current healthcare system structures and processes to support family dementia caregivers within routine care practices.

Trial registration

ClinicalTrials.gov NCT04037501. Registered on 30 July 2019.
melRNA-seq for Expression Analysis of SINE RNAs and Other Medium-Length Non-Coding RNAs
Authors: Mori Yoshinobu , Ichiyanagi Kenji
Published: June 2021

Background

Small interspersed elements (SINEs) are transcribed by RNA polymerase III (Pol III) to produce RNAs typically 100–500 nucleotides in length. Although their RNA abundance can be evaluated by Northern blotting and primer extension, the nature (sequence, exact length, and genomic origin) of these RNAs cannot be revealed by these methods. Moreover, mRNA sequencing (mRNA-seq) is not able to distinguish bona fide SINE RNAs or SINE sequences present in longer transcripts.

Results

To elucidate the abundance, source loci, and sequence nature of SINE RNAs, we established a deep sequencing method, designated as melRNA-seq (medium-length RNA-seq), which can determine whole-length RNA sequences. Total RNA samples were treated with 5′ pyrophosphohydrolase (RppH), which allowed ligation of an RNA adaptor to the 5′ end of intact SINE RNAs. Similarly, another adaptor was ligated to the 3′ end, followed by reverse transcription, PCR amplification, size selection, and single-end deep sequencing. The analysis of two biological replicates of RNAs from mouse spermatogonia showed high reproducibility of SINE expression data both at family and locus levels.

Conclusions

This new method can be used for quantification and detailed sequence analysis of medium-length non-coding RNAs, such as rRNA, snRNA, tRNAs, and SINE RNAs. Further, its dynamic range is much wider than Northern blotting and primer extension.
Real-time embedded object detection and tracking system in Zynq SoC
Authors: Ji Qingbo , Dai Chong , Hou Changbo , Li Xun
Published: June 2021

With the increasing application of computer vision technology in autonomous driving, robot, and other mobile devices, more and more attention has been paid to the implementation of target detection and tracking algorithms on embedded platforms. The real-time performance and robustness of algorithms are two hot research topics and challenges in this field. In order to solve the problems of poor real-time tracking performance of embedded systems using convolutional neural networks and low robustness of tracking algorithms for complex scenes, this paper proposes a fast and accurate real-time video detection and tracking algorithm suitable for embedded systems. The algorithm combines the object detection model of single-shot multibox detection in deep convolution networks and the kernel correlation filters tracking algorithm, what is more, it accelerates the single-shot multibox detection model using field-programmable gate arrays, which satisfies the real-time performance of the algorithm on the embedded platform. To solve the problem of model contamination after the kernel correlation filters algorithm fails to track in complex scenes, an improvement in the validity detection mechanism of tracking results is proposed that solves the problem of the traditional kernel correlation filters algorithm not being able to robustly track for a long time. In order to solve the problem that the missed rate of the single-shot multibox detection model is high under the conditions of motion blur or illumination variation, a strategy to reduce missed rate is proposed that effectively reduces the missed detection. The experimental results on the embedded platform show that the algorithm can achieve real-time tracking of the object in the video and can automatically reposition the object to continue tracking after the object tracking fails.
Optimal control for obstacle problems involving time-dependent variational inequalities with Liouville–Caputo fractional derivative
Authors: Sa Ngiamsunthorn Parinya , Suechoei Apassara , Kumam Poom
Published: June 2021

We consider an optimal control problem for a time-dependent obstacle variational inequality involving fractional Liouville–Caputo derivative. The obstacle is considered as the control, and the corresponding solution to the obstacle problem is regarded as the state. Our aim is to find the optimal control with the properties that the state is closed to a given target profile and the obstacle is not excessively large in terms of its norm. We prove existence results and establish necessary conditions of obstacle problems via the approximated time fractional-order partial differential equations and their adjoint problems. The result in this paper is a generalization of the obstacle problem for a parabolic variational inequalities as the Liouville–Caputo fractional derivatives were used instead of the classical derivatives.
Combined Indeterminate and Impaired Glucose Tolerance Is a Novel Group at High Risk of Cystic Fibrosis-Related Diabetes
Authors: Potter Kathryn J , Reynaud Quitterie , Boudreau Valérie , Racine Florence , Tremblay François , Lavoie Annick , Carricart Maite , Mailhot Geneviève , Durieu Isabelle , Senior Peter A , Rabasa-Lhoret Rémi
Published: June 2021

Background

Indeterminate glycemia (INDET) and impaired glucose tolerance (IGT) are independently associated with cystic fibrosis-related diabetes (CFRD) risk. We determined whether patients meeting both criteria have increased risk of diabetes in 2 separate adult cohorts.

Methods

The Montreal Cystic Fibrosis Cohort (MCFC; n = 293 baseline and 198 for prospective analysis excluding subjects identified with incident CFRD at baseline) and the Lyon cystic fibrosis cohort [Determination of the Predictive Factors in the Reversibility or the Aggravation in the Disorders of the Glucose Metabolism in Cystic Fibrosis Patients (DIAMUCO); n = 144/105] are prospective observational cohorts.

Results

In the MCFC and DIAMUCO cohorts, mean age was 25.5 ± 7.7 and 25.0 ± 8.6 years; body mass index, 21.7 ± 3.0 and 20.2 ± 2.2 kg/m2; percentage of forced expiratory volume expired in 1 sec, 73.2 ± 22.1 and 62.5 ± 21.9; and follow-up, 6.9 ± 3.8 and 2.4 ± 1.2 years, respectively. In the MCFC cohort, the IGT only and combined INDET and IGT (INDET + IGT) groups had greater risk of CFRD (P = 0.0109). In the DIAMUCO cohort, there was lower diabetes-free survival in the INDET + IGT group (P = 0.0105). In both cohorts, CFRD risk ranged from 17% in normal glucose tolerance patients up to 42% to 56% in patients with INDET + IGT.

Conclusion

Patients who meet combined criteria have a higher risk of developing diabetes probably justifying closer follow-up.
Decreased KATP Channel Activity Contributes to the Low Glucose Threshold for Insulin Secretion of Rat Neonatal Islets
Authors: Yang Juxiang , Hammoud Batoul , Li Changhong , Ridler Abigail , Yau Daphne , Kim Junil , Won Kyoung-Jae , Stanley Charles A , Hoshi Toshinori , Stanescu Diana E
Published: June 2021

Transitional hypoglycemia in normal newborns occurs in the first 3 days of life and has clinical features consistent with hyperinsulinism. We found a lower threshold for glucose-stimulated insulin secretion from freshly isolated embryonic day (E) 22 rat islets, which persisted into the first postnatal days. The threshold reached the adult level by postnatal day (P) 14. Culturing P14 islets also decreased the glucose threshold. Freshly isolated P1 rat islets had a lower threshold for insulin secretion in response to 2-aminobicyclo-(2, 2, 1)-heptane-2-carboxylic acid, a nonmetabolizable leucine analog, and diminished insulin release in response to tolbutamide, an inhibitor of β-cell KATP channels. These findings suggested that decreased KATP channel function could be responsible for the lower glucose threshold for insulin secretion. Single-cell transcriptomic analysis did not reveal a lower expression of KATP subunit genes in E22 compared with P14 β cells. The investigation of electrophysiological characteristics of dispersed β cells showed that early neonatal and cultured cells had fewer functional KATP channels per unit membrane area. Our findings suggest that decreased surface density of KATP channels may contribute to the observed differences in glucose threshold for insulin release.
The Ethical Life of Counter-Communities
Authors: Loick Daniel
Published: June 2021

This article explores the normative structure of counter-communities. These communities do not simply fulfill a compensatory function for excluded or oppressed groups; their specific sociality is transcendent of society at large. The article outlines this notion of the “superiority of the subjugated” with Hegel and explains its social-theoretical relevance by turning to Marx and Engels. This tradition of thought offers two explanations for the superiority of subjugated sociality, one that relies on a philosophy of history (the servant's way of existence anticipates a coming sociality), and one that relies on anthropological premises (servants have an already-present access to specific social potentials of their species being that the masters are missing). After considering these two options with regard to the proletariat as a form of subjugated sociality, the article makes the case for a general theory of the ethical life of counter-communities and applies it to the examples of queer and diasporic forms of collectivity. In closing, the article explores the success conditions of counter-collectivization as well as names some social-theoretical implications.
Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2
Authors: Balaraju Anurag Kakkerla , Hu Bo , Rodriguez Juan J. , Murry Matthew , Lin Fang
Published: June 2021

Noncanonical Wnt/planar cell polarity (Wnt/PCP) signaling has been implicated in endoderm morphogenesis. However, the underlying cellular and molecular mechanisms of this process are unclear. We found that, during convergence and extension (C&E) in zebrafish, gut endodermal cells are polarized mediolaterally, with GFP-Vangl2 enriched at the anterior edges. Endoderm cell polarity is lost and intercalation is impaired in the absence of glypican 4 (gpc4), a heparan-sulfate proteoglycan that promotes Wnt/PCP signaling, suggesting that this signaling is required for endodermal cell polarity. Live imaging revealed that endoderm C&E is accomplished by polarized cell protrusions and junction remodeling, which are impaired in gpc4-deficient endodermal cells. Furthermore, in the absence of gpc4, Cadherin 2 expression on the endodermal cell surface is increased as a result of impaired Rab5c-mediated endocytosis, which partially accounts for the endodermal defects in these mutants. These findings indicate that Gpc4 regulates endodermal planar cell polarity during endoderm C&E by influencing the localization of Cadherin 2. Thus, our study uncovers a new mechanism by which Gpc4 regulates planar cell polarity and reveals the role of Wnt/PCP signaling in endoderm morphogenesis.
Entre los hermanos Lumiére y TikTok. Apuntes didácticos para una educación audiovisual en la infancia.
Authors: Augustowsky Gabriela
Published: June 2021

En un contexto social con alta disponibilidad y accesibilidad tecnológica, este artículo convoca a discutir acerca de los propósitos, contenidos, estrategias y sentidos de una educación audiovisual contemporánea para niñas y niños. Para esto, se presentan tres perspectivas teóricas: el cine como arte, encuentros con la alteridad y la experiencia creadora; el cine como lenguaje y la alfabetización en medios audiovisuales; la creación audiovisual para la democratización de saberes, la inclusión social y la ampliación de derechos. Frente a las estrategias de corte analítico, defensivas o centradas en aspectos tecnológicos, se propone vincular el universo audiovisual con la creación artística y con la rica tradición cultural del cine y su historia. Así se abordan: las experimentaciones lúdicas con el movimiento y la luz; los dispositivos de creación audiovisual (El Minuto Lumiére) y el cine de animación cuadro a cuadro. Finalmente, se trata la educación audiovisual como un campo en construcción y sus posibilidades para una labor docente transformadora.
Case Article—Route Planning at an Animal Husbandry Department
Authors: Choudhari Sanjay , Chandra Saurabh
Published: June 2021

The case provides an opportunity for undergraduate, graduate, and doctoral students to demonstrate a practical application of supply chain modeling in the area of livestock management for a group of rural communities in India. An animal husbandry department needs to ensure timely replenishment of liquid nitrogen to several livestock locations to preserve semen straws, which are frozen for artificial insemination. The department wishes to explore a possible route redesign for cost reduction. The description in the case, as well as the presented data, is based on a real project undertaken by the authors. The case enables students to conceptualize and design sample routes manually to meet the cost objective. However, the main objective is to make students understand and implement solution heuristics for large-scale vehicle routing problems. It also allows students to compare various designs trying not only possible vehicle capacities but also three solution heuristics. Nevertheless, students can also realize the importance of the spreadsheet as a decision support tool for evaluating several options by integrating the chosen heuristic. An important milestone of the case project was the implementation of our recommendations by the animal husbandry department.
Investigation of Third Grade Students' Views on Media Literacy
Authors: Arslan-Cansever Belgin
Published: June 2021

The purpose of this study is to examine the views of nine years old students about media literacy and media literate. Phenomenology approach was used qualitative research designs in the research. The study group consisted of 12 (7 girls, 5 boys) third grade students from two different primary schools in Bayrakli, Izmir, Turkey. Research data were collected through interviews. In these interviews, a semi-structured interview form was used. Content analysis was used to analyze the data. Research findings are grouped under three themes: The meaning of media literacy, Characteristics of media literate and Being able to define themselves as being media literates or not. The findings showed that students mostly defined media literacy as “having a time limit in the use of media tools” and “critical view to the media”. According to students, the media literate is “ the one who uses the media for educational purposes" and as "critics".
Vortex and symmetric radiation character of nonlinear Thomson scattering in Laguerre-Gaussian circularly polarized laser pulses
Authors: Wang Yiqiu , Zhou Qinyan , Zhuang Jiawei , Yu Penghang , Tian Youwei
Published: June 2021

The radiation character of nonlinear Thomson scattering is investigated in the
interaction of Laguerre-Gaussian circularly polarized laser pulses
with a single electron in the angular plane. With theoretical analysis
and numerical calculation, it is shown that the angular radiation
distributions have annular structures with great fourfold or plane
symmetry in pulses characterized by comparatively lower laser
intensity (a0 < 6),
prolonged pulse duration (τ > 50fs)or wide beam waist
(b0 > 5μm). In other
circumstances, a vortex radiation pattern is found for the first time
on the basis of the electron dynamics. Further, by increasing the
initial phase of laser pulse, the overall angular radiation has an
interesting counter-clockwise rotating trend with a cycle of Δξ0 = 2π. These results would help the understanding of nonlinear
Thomson scattering and push forward the research of twisted X/γ-ray
generation in optical laboratory.
Third-order harmonic generation in a bi-chromatic elliptical laser field
Authors: He Tao , Zhang Yizhu , Zhao J. J. , Wang Xincheng , Shen Zhenjie , Jin Zuanming , Yan Tian-Min , Jiang Yuhai
Published: June 2021

The low-order harmonic generation induced by a strong laser field produces a bright, ultrashort, supercontinuum radiation ranging from the terahertz to ultraviolet band. By controlling the phase-delay and ellipticity of the bi-chromatic laser fields, the third harmonic generation is experimentally and theoretically investigated for elucidating the mechanism of the low-order harmonics. The third harmonic generation is found to be strongly suppressed in the counter-rotating bi-chromatic laser field due to the selection rule for harmonic emissions. The continuum-continuum transition in the strong field approximation is extended to explain the third harmonic generation as a function of the phase delay and ellipticity of the bi-chromatic laser fields. Compared with the semi-classical photocurrent model, the continuum-continuum transition on the basis of quantum-mechanical treatment achieves better agreement with the experimental observations. Our work indicates that the overlapping in continuum states via different quantum paths of a single electron plays a role in low-order harmonics generation under elliptical bi-chromatic laser fields.