bluuebunny/crossref_metadata_2025 huggingface dataset:
trips4health: a single-blinded randomised controlled trial incentivising adult public transport use for physical activity gain
Authors: Evans Jack. T. , Stanesby Oliver , Blizzard Leigh , Jose Kim , Sharman Melanie J. , Ball Kylie , Greaves Stephen , Palmer Andrew J. , Cooper Katie , Gall Seana L. , Cleland Verity J.
Published: August 2023

Background

Public transport users tend to accumulate more physical activity than non-users; however, whether physical activity is increased by financially incentivising public transport use is unknown. The trips4health study aimed to determine the impact of an incentive-based public transport intervention on physical activity.

Methods

A single-blinded randomised control trial of a 16-week incentive-based intervention involved Australian adults who were infrequent bus users (≥ 18 years; used bus ≤ 2 times/week) split equally into intervention and control groups. The intervention group were sent weekly motivational text messages and awarded smartcard bus credit when targets were met. The intervention group and control group received physical activity guidelines. Accelerometer-measured steps/day (primary outcome), self-reported transport-related physical activity (walking and cycling for transport) and total physical activity (min/week and MET-min/week) outcomes were assessed at baseline and follow-up.

Results

Due to the COVID pandemic, the trial was abandoned prior to target sample size achievement and completion of all assessments (N = 110). Steps/day declined in both groups, but by less in the intervention group [-557.9 steps (-7.9%) vs.-1018.3 steps/week (-13.8%)]. In the intervention group, transport-related physical activity increased [80.0 min/week (133.3%); 264.0 MET-min/week (133.3%)] while total physical activity levels saw little change [35.0 min/week (5.5%); 25.5 MET-min/week (1.0%)]. Control group transport-related physical activity decreased [-20.0 min/week (-27.6%); -41.3 MET-min/week (-17.3%)], but total physical activity increased [260.0 min/week (54.5%); 734.3 MET-min/week (37.4%)].

Conclusion

This study found evidence that financial incentive-based intervention to increase public transport use is effective in increasing transport-related physical activity These results warrant future examination of physical activity incentives programs in a fully powered study with longer-term follow-up.

Trial registration

This trial was registered with the Australian and New Zealand Clinical Trials Registry August 14th, 2019: ACTRN12619001136190; https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=377914&isReview=true
Microglial senescence contributes to female-biased neuroinflammation in the aging mouse hippocampus: implications for Alzheimer’s disease
Authors: Ocañas Sarah R. , Pham Kevin D. , Cox Jillian E. J. , Keck Alex W. , Ko Sunghwan , Ampadu Felix A. , Porter Hunter L. , Ansere Victor A. , Kulpa Adam , Kellogg Collyn M. , Machalinski Adeline H. , Thomas Manu A. , Wright Zsabre , Chucair-Elliott Ana J. , Freeman Willard M.
Published: August 2023

Background

Microglia, the brain’s principal immune cells, have been implicated in the pathogenesis of Alzheimer’s disease (AD), a condition shown to affect more females than males. Although sex differences in microglial function and transcriptomic programming have been described across development and in disease models of AD, no studies have comprehensively identified the sex divergences that emerge in the aging mouse hippocampus. Further, existing models of AD generally develop pathology (amyloid plaques and tau tangles) early in life and fail to recapitulate the aged brain environment associated with late-onset AD. Here, we examined and compared transcriptomic and translatomic sex effects in young and old murine hippocampal microglia.

Methods

Hippocampal tissue from C57BL6/N and microglial NuTRAP mice of both sexes were collected at young (5–6 month-old [mo]) and old (22–25 mo) ages. Cell sorting and affinity purification techniques were used to isolate the microglial transcriptome and translatome for RNA-sequencing and differential expression analyses. Flow cytometry, qPCR, and imaging approaches were used to confirm the transcriptomic and translatomic findings.

Results

There were marginal sex differences identified in the young hippocampal microglia, with most differentially expressed genes (DEGs) restricted to the sex chromosomes. Both sex chromosomally and autosomally encoded sex differences emerged with aging. These sex DEGs identified at old age were primarily female-biased and enriched in senescent and disease-associated microglial signatures. Normalized gene expression values can be accessed through a searchable web interface (https://neuroepigenomics.omrf.org/). Pathway analyses identified upstream regulators induced to a greater extent in females than in males, including inflammatory mediators IFNG, TNF, and IL1B, as well as AD-risk genes TREM2 and APP.

Conclusions

These data suggest that female microglia adopt disease-associated and senescent phenotypes in the aging mouse hippocampus, even in the absence of disease pathology, to a greater extent than males. This sexually divergent microglial phenotype may explain the difference in susceptibility and disease progression in the case of AD pathology. Future studies will need to explore sex differences in microglial heterogeneity in response to AD pathology and determine how sex-specific regulators (i.e., sex chromosomal or hormonal) elicit these sex effects.
The top 100 cited articles in menstrual health among adolescent girls: a citation analysis
Authors: Alekhya G. , Sahu Dinesh Prasad , Behera Priyamadhaba
Published: August 2023

Background

Menstrual health is an important public health concern where it is still considered a taboo, and adolescent girls often lack knowledge about menstrual health, face limited access to sanitation facilities, and struggle with the affordability of sanitary materials. Every year numerous articles are published; however, only a few of them would be influential in the evolution of a particular field. The number of citations received by an article serves as a quality factor for the impact of the article in a particular field. Citation analysis analyses the relationship between citations received by articles. From the literature search, no citation analysis was conducted on menstrual health. Hence the objective of the study was to identify the articles which received hundred or more citations and also to identify the leading countries, journals, study designs, and departments conducting research on menstrual health.

Methods

Citation analysis was done with search terms pertaining to adolescent and menstrual health using Google Scholar as a database in Publish or Perish software. The articles retrieved were exported to Microsoft Excel. Articles that received a hundred or more citations were screened for the type of article, department, and country where the study was conducted. A descriptive analysis of the hundred or more cited articles was done in Microsoft Excel.

Results

A total of 982 articles pertaining to menstrual health among adolescent girls were retrieved. There were hundred articles with hundred and more citations pertaining to the menstrual health of adolescent girls. Cross-sectional study design, Obstetrics and Gynaecology department, India and USA countries, and PLOS ONE journal had the most citations in research on menstrual health among adolescent girls. The top ten articles were on menorrhagia, menstrual hygiene practices, Water, Sanitation and hygiene (WASH), stigma on menstruation, and education on menstrual health.

Conclusion

The hundred cited articles on menstrual health among adolescent girls were mainly from high-income countries and were of more observational in nature than interventional. Thus, highlighting the need to strengthen experimental studies on the menstrual health of adolescent girls in Lower-middle-income countries.
Mechanical properties of an elastically deformable cervical spine implant
Authors: Abudouaini Haimiti , Wu Tingkui , Meng Yang , Wang Beiyu , Liu Hao
Published: August 2023

Anterior cervical surgery is widely accepted and time-tested surgical procedure for treating cervical radiculopathy and myelopathy. However, there is concern about the high adjacent segment degeneration rate and implant subsidence after the surgery using the traditional polyetheretherketone cage. Thus, we creatively designed a polyurethane cervical implant that can continuous load-sharing through elastic deformation and decrease postoperative stress concentration at adjacent segments. In this study, the design rationality and safety of this novel implant was evaluated based on several mechanical parameters including compression test, creeping test, push-out test and subsidence test. The results showed that the novel cervical implant remained intact under the compressive axial load of 8000 N and continues to maintained the elastic deformation phase. The minimum push-out load of the implant was 181.17 N, which was significantly higher than the maximum compressive shear load of 20 N experienced by a normal human cervical intervertebral disc. Besides, the creep recovery behaviour of the implant closely resembled what has been reported for natural intervertebral discs and clinically applied cervical devices in literature. Under the load of simulating daily activities of the cervical spine, the implant longitudinal displacement was only 0.54 mm. In conclusion, this study showed that the current design of the elastically deformable implant was reasonable and stable to fulfil the mechanical requirements of a cervical prosthesis under physiological loads. After a more comprehensive understanding of bone formation and stress distribution after implantation, this cervical implant is promising to be applied to certain patients in clinical practice.
Host–pathogen associations revealed by genotyping of European strains of Anaplasma phagocytophilum to describe natural endemic cycles
Authors: Fröhlich Julia , Fischer Susanne , Bauer Benjamin , Hamel Dietmar , Kohn Barbara , Ahlers Marion , Obiegala Anna , Overzier Evelyn , Pfeffer Martin , Pfister Kurt , Răileanu Cristian , Rehbein Steffen , Skuballa Jasmin , Silaghi Cornelia
Published: August 2023

Background

The zoonotic intracellular alpha-proteobacterium Anaplasma phagocytophilum is a tick-transmitted pathogen. The associations between vertebrate reservoirs and vectors are described as wide-ranging, and it was previously shown that the pathogenicity of A. phagocytophilum differs depending on the combination of pathogen variant and infected host species. This leads to the question of whether there are variations in particular gene loci associated with different virulence. Therefore, this study aims at clarifying existing host-variant combinations and detecting possible reservoir hosts. To understand these interactions, a complex toolset for molecular epidemiology, phylogeny and network theory was applied.

Methods

Sequences of up to four gene loci (msp4, msp2, groEL and 16S rRNA) were evaluated for different isolates from variable host species, including, for example, dogs, cattle and deer. Variant typing was conducted for each gene locus individually, and combinations of different gene loci were analysed to gain more detailed information about the genetic plasticity of A. phagocytophilum. Results were displayed as minimum spanning nets and correlation nets.

Results

The highest diversity of variants for all gene loci was observed in roe deer. In cattle, a reduced number of variants for 16S rRNA [only 16S-20(W) and 16S-22(Y)] but multiple variants of msp4 and groEL were found. For dogs, two msp4 variants [m4-20 and m4-2(B/C)] were found to be linked to different variants of the other three gene loci, creating two main combinations of gene loci variants. Cattle are placed centrally in the minimum spanning net analyses, indicating a crucial role in the transmission cycles by possibly bridging the vector-wildlife cycle to infections of humans and domestic animals. The minimum spanning nets confirmed previously described epidemiological cycles of the bacterium in Europe, showing separation of variants originating from wildlife animals only and a set of variants shared by wild and domestic animals.

Conclusions

In this comprehensive study of 1280 sequences, we found a high number of gene variants only occurring in specific hosts. Additionally, different hosts show unique but also shared variant combinations. The use of our four gene loci expand the knowledge of host–pathogen interactions and may be a starting point to predict future spread and infection risks of A. phagocytophilum in Europe.

Graphical abstract
Cranial and endocranial comparative anatomy of the Pleistocene glyptodonts from the Santiago Roth Collection
Authors: Christen Zoe M. , Sánchez-Villagra Marcelo R. , Le Verger Kévin
Published: August 2023

With their odd cranial features, glyptodonts, closely related to extant armadillos, are a highly diverse group of the South American megafauna. Doedicurus, Glyptodon, Panochthus, and Neosclerocalyptus were present in the “Pampean Formation” during the Pleistocene, and they are all exceptionally preserved in the Santiago Roth Collection, thus offering the possibility of investigating these four well-diversified genera. A total of 13 specimens (seven species) were analysed and compared in a qualitative/quantitative study of external cranial remains and endocranial reconstructions (i.e., braincase and associated cranial canals, and inner ears). We report on anatomical features that contribute to existing phylogenetic matrices; many of them are new potential synapomorphies supporting the current hypotheses regarding the evolutionary history of the Pleistocene glyptodonts. These include the anterior cranial shape, the position of the basicranium in respect to the whole cranium, the shape of the cranial roof, the position of the largest semicircular canal, and the inclination of the cerebrum. They may represent new shared-derived features among Glyptodon, Doedicurus, Neosclerocalyptus, and Panochthus. We also provide detailed comparative descriptions highlighting new potential convergences in respect to current phylogenies, concerning, for instance, the shape of the foramen magnum, the global shape of the cranium, orbital shape, cochlear position, and a strong protrusion of the zygomatic process of the squamosal. In light of these results, we discuss morphological transformations across phylogeny. The endocranial comparison brought insights on the phylogenetic patterns of cranial canal evolution.
Bootstrapped low complex iterative LDPC decoding algorithms for free-space optical communication links
Authors: Youssef Albashir A.
Published: August 2023

In recent years, much research has been devoted to free-space optical communication (FSO). The unregulated spectrum, low implementation costs, and robust security of FSO systems all are of great importance which lead to a wide range of applications for FSO links, from terrestrial communications to satellite communications. However, the fundamental limitation with FSO links is atmospheric turbulence (AT) caused by fading, significantly reducing link performance. Random phenomena are the best characteristic of atmospheric turbulence caused by changes in the air’s refractive index over time. Numerous probability density functions of the AT models were presented to model the randomness in AT channels. The Log-Normal (LN) channel model is for weak atmospheric turbulence, while the Gamma–Gamma (G–G) channel is selected for moderate and strong atmospheric turbulence. The impacts of geometric losses, attenuation due to weather, and errors due to misalignment are addressed using LN and G–G channels. Channel coding is one of the possible solutions for mitigating such FSO channel impairments as the low-density parity check (LDPC) codes. In this article, the Weighted Bit Flipping (Algorithm (1)), Implementation Efficient Reliability Ratio Weighted Bit Flipping (Algorithm (2)), and Min-Sum (Algorithm (3)) algorithms are compared and evaluated against FSO atmospheric turbulence channels. In addition, two novel algorithms are proposed to enhance the complexity or Bit Error Rate performance of LDPC decoding over FSO channels. The results showed an impressive improvement of the coded FSO system by employing the proposed algorithms compared to the existing LDPC decoders for FSO communications from the point of all comparison parameters.
Performance analysis of wireless compressed-image transmission over DST-based OFDMA systems
Authors: Al-Ashwal Nagi H. , Al Soufy Khaled A. M. , Al-Kamali Faisal S. , Swillam Mohamed
Published: August 2023

Multimedia data, like images, consumes significant bandwidth when transmitted over wireless systems. Therefore, compressing transmitted images becomes crucial to reduce the required bandwidth and improve energy efficiency. This work aims to analyze the performance of transmitting wireless compressed images over a recent Discrete Sine Transform (DST)-Based Orthogonal Frequency Division Multiple Access (DST-OFDMA) system. It investigates the effectiveness of several image compression methods by determining the minimum Signal-to-Noise Ratio (SNR) required for each method to achieve error-free image recovery at the receiver. This work considers different modulation schemes including 16QAM and QPSK, as well as different subcarrier mapping schemes (localized and interleaved) over vehicular A, SUI3, and uniform channels. Nine standard compression methods are used for analyzing the performance of the DST-OFDMA system and compared it with that of the conventional Discrete Fourier Transform (DFT)-based OFDMA (DFT-OFDMA) system. The results show that the performance of DST-OFDMA outperforms that of DFT-OFDMA, especially when QPSK modulation is used. Simulation results demonstrate that the interleaved DST-OFDMA (DST-IOFDMA) system, employing the SPIHT_3D compression method and QPSK modulation (over the SUI3 channel model), achieves the lowest SNR value required for compressed image recovery, approximately 18 dB. This indicates that the SPIHT_3D compression method exhibits lower power consumption compared to other methods as well as high bandwidth efficiency.
A Q-learning-based distributed queuing Mac protocol for Internet-of-Things networks
Authors: Wu Chien-Min , Chang Yu-Hsiu , Tsai Cheng-Tai , Hou Cheng-Chun
Published: August 2023

Many previous studies showed that some nodes should be in a sleeping state while the traffic is high. These nodes can wake up periodically to transmit data while they have data to transmit. The system throughput will be increased while each node can change between wake-up and sleeping states periodically. If a node can estimate the active rate and wake up at the optimal time, the collision probability decreases. This study proposes a Q-learning-based distributed queuing medium access control (QL-based DQMAC) protocol for Internet-of-Things (IoT) networks. In the proposed QL-based DQMAC, we derive the optimal number of contention IoT nodes. Each node calculates the active rate by itself through the Q-learning algorithm. Then, each node determines whether it will be active or in sleeping mode in the next contention period according to the active rate. Finding the optimal IoT nodes in each contention period decreases the probability of collision. The energy consumption due to the contention and delay for MAC contention is reduced owing to the lower number of contentions. Protocol comparison with other DQMAC protocols shows that the proposed QL-based DQMAC protocol achieves higher performance in IoT networks.
A qualitative study of interest in and preferences for potential medications to treat methamphetamine use disorder
Authors: Wagner Karla D. , Marks Charles , Fiuty Phillip , Harding Robert W. , Page Kimberly
Published: August 2023

Introduction

We examined acceptability of and preferences for potential medications for treating methamphetamine use disorder (MUD) among people who use methamphetamine and examined how benefits and drawbacks of methamphetamine use affect perceived acceptability and preferences.

Methods

We conducted qualitative interviews as part of a larger study in 2019–2020. The interview assessed patterns of substance use (including methamphetamine), benefits and drawbacks of methamphetamine use, and interest in a medication to treat MUD. Analysis used an inductive thematic approach, guided by three primary questions: (1) would participants be interested in taking a potential medication for MUD?; (2) what effects would they would like from such a medication?; and (3) what would their ideal treatment route and schedule be (e.g. daily pill, monthly injection)?.

Results

We interviewed 20 people reporting methamphetamine use in the past 3 months (10 from Reno, Nevada, USA and 10 from Rio Arriba County, New Mexico, USA). Seven used exclusively methamphetamine, while thirteen used other substances in addition to methamphetamine. Most were enthusiastic about a potential medication to treat MUD. Of those who were not interested (n = 5), all indicated no current concerns about their methamphetamine use. Perceived functional benefits of methamphetamine use (i.e., energy, counteracting opioid sedation, and improved social and emotional wellbeing) informed preferences for a replacement-type medication that would confer the same benefits while mitigating drawbacks (e.g., psychosis, hallucinations, withdrawal). Opinions on preferred dosing varied, with some preferring longer acting medications for convenience, while others preferred daily dosing that would align with existing routines.

Conclusion

Participants were excited about a potential for a medication to treat MUD. Their preferences were informed by the functional role of methamphetamine in their lives and a desire to maintain the stimulant effects while mitigating harms of illicit methamphetamine. Treatment outcomes that emphasize functioning and wellbeing, rather than abstinence, should be explored.
The impact of methamphetamine use on medications for opioid use disorder (MOUD) treatment retention: a scoping review
Authors: Russell Cayley , Law Justine , Imtiaz Sameer , Rehm Jürgen , Le Foll Bernard , Ali Farihah
Published: August 2023

Background

An emerging public health threat of methamphetamine/opioid co-use is occurring in North America, including increases in overdoses related to concomitant methamphetamine/opioid use. This presents a potential risk to established treatments for opioid use disorder (i.e., medications for opioid use disorder [MOUD]). To date, few studies have examined the impact of methamphetamine use on MOUD-related outcomes, and no studies have synthesized data on MOUD retention.

Methods

A scoping review was undertaken to examine the impact of methamphetamine use on MOUD retention. All original published research articles were searched in Embase, MEDLINE, PsychINFO, CINAHL, Scopus, Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews and Cochrane Protocols, and Google scholar databases. Data were extracted into a standardized data extraction chart. Findings were presented narratively.

Results

All eight included studies demonstrated an increased likelihood of treatment discontinuation or dropout among patients enrolled in MOUD who used methamphetamine. The frequency of methamphetamine use was also associated with MOUD dropout, in that those who used methamphetamine more often were more likely to discontinue MOUD. The definitions and measurements of MOUD retention varied considerably, as did the magnitude of effect size.

Conclusions

Results indicate that methamphetamine use has an undesirable impact on MOUD retention and results in an increased risk of treatment discontinuation or dropout. Strategies to identify concurrent methamphetamine use among individuals engaging in MOUD and educate them on the increased risk for dropout should be undertaken. Further research is needed to understand how MOUD retention among patients with concomitant opioid and methamphetamine use can be improved.
Evaluation of the QuEChERS extraction approach for the analysis of active compounds of pharmaceuticals in olive tree portions
Authors: Brunetti Gennaro , Traversa Andreina , De Mastro Francesco , Dichio Bartolomeo , Mottola Francesca , Mininni Alba N. , Nigro Pietro , Cocozza Claudio
Published: August 2023

Background

A modified version of the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method has been tested for quantifying six active compounds of pharmaceuticals (PhACs), i.e., two different antibiotics, two anti-inflammatories, one antifungal, and one anti-depressant, extracted from roots, leaves and stems, pulp, pits, and oil obtained from olive trees. The different matrices have been polluted with all contaminants at 25, 50, and 250 µg L−1 and the recoveries were determined by liquid chromatography tandem–mass spectrometry. The validation of the method has been carried out by determining linearity, recovery, precision, limits of detection (LODs), and limits of quantification (LOQs) values. A matrix-matched calibration for each matrix has been adopted in order to avoid the matrix effect at the aforementioned levels of fortification.

Results

The recoveries of PhACs from the different matrices were always above 70% and the relative standard deviation (RSD) always ≤ 20%, conditions required for the validation of the method. The LOD and LOQ values were always lower than 25 µg L−1, i.e., always lower than the minimum concentration used in the experiment; therefore, the method can be validated at 25, 50, and 250 µg L−1.

Conclusions

This method can represent a valid alternative to the traditional extraction methods to quantify pharmaceuticals extracted also from fatty matrices.

Graphical Abstract
First report of inhibitory abilities of dark septate endophytic fungi against white root rot disease on Hevea brasiliensis seedlings in nursery conditions
Authors: Dalimunthe Cici Indriani , Surono  , Soekarno Bonny Poernomo Wahyu , Al-Ani Laith Khalil Tawfeeq , Munif Abdul , Sriherwanto Catur , Nurdebyandaru Nicho
Published: August 2023

Background

The dark septate endophytes (DSE) are endophytic and non-mycorrhizal fungi with the ability to impact and control some plant pathogens and promote plant growth. The aim of this study was to investigate the effectiveness of five different DSE fungal isolates in controlling white root rot disease (WRRD) caused by the plant pathogen, Rigidoporus microporus in Hevea brasiliensis in a nursery system. There are no previous reports on the role of DSE in controlling WRRD. In this study, the efficacy of five DSE isolates, including Acrocalymma vagum SBTBMDS 1, Clonostachys chloroleuca TMDS 2.1, Lasiodiplodia theobromae APDS 3.2, Penicillium oxalicum TMDS 3.2, and Fusarium falciforme TBMDS 2.4b, was tested for their ability to reduce the severity of WRRD in vivo.

Results

The results showed that all the DSE isolates were able to inhibit R. microporus, leading to a decrease in the disease severity of WRRD, with percentages ranging from 7.50 to 17.5% and percentage of disease inhibition from 57.67 to 83.33% than the controls, which had a severity percentage of 45%. The TMDS3.2 isolate showed high efficacy in increasing the girth (137%) and height (63.3%) of H. brasiliensis seedlings, while the isolates of P. oxalicum TMDS 3.2 and F. falciforme TMDS 2.4b enhanced the dry weight (123 and 122%, respectively) than the control. Two isolates of P. oxacilum TMDS 3.2 and C. chloroleuca TMDS 2.1 increased the root volume (120 and 107%, respectively) than the control. Gas chromatography–mass spectrometry (GC–MS) analysis showed the ability of three selected DSE isolates, L. theobromae APDS 3.2, F. falciforme TBMDS 2.4b, and P. oxacilum TMDS 3.2, to produce acetic acid butyl methyl-phosphinoylmethyl ester and ethanone, 1-(4-methyl-1H(imidazol-2-yl)—that previously reported as antimicrobials.

Conclusion

This study demonstrated that DSE fungal isolates had the potential to act as a biocontrol agent against R. microporus in H. Brasiliensis seedlings. In the future, the findings of this study could be utilized to prevent WRRD, one of the most serious problems in rubber plantations, in an environmentally friendly way by reducing the usage of fungicides.
Endophytic establishment of native Bacillus thuringiensis strain in maize plants and its efficacy against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Authors: Karshanal J. , Kalia Vinay K.
Published: August 2023

Background

Bacillus thuringiensis (Bt) is known as the most successful microbial insecticide worldwide used against lepidopteran insect pests in agriculture. Native Bacillus isolate VKK5 showing insecticidal activity against Spodoptera frugiperda (FAW) (J.E. Smith) (Lepidoptera: Noctuidae) was characterized as B. thuringiensis (BtVKK5) on a morphological and molecular basis. Recent research has shown that Bt can be established as an endophytic organism for controlling insect pests. The present work aimed at assessing the colonization of BtVKK5 as an endophyte in five maize cultivars by seed treatment (ST), soil drenching (SD), foliar application (FA) and combination of all methods (ST + SD + FA) and its bioefficacy against neonates of FAW.

Results

Establishment of inoculated BtVKK5 as endophytes in five maize cultivars, viz. Pusa HQPM7 Improved, Pusa Jawahar Hybrid Maize 1, Pusa Vivek Hybrid 27 Improved (PVH27I), Pusa HQPM5 Improved and DMRH 1301, was confirmed by re-isolating from the leaves of the plant on ampicillin-selected agar plates. Estimation of colony-forming units per gram of leaf showed that there was a significant difference in colonization of the Bt strain among maize cultivars by different inoculation methods. The colonies were further substantiated by the amplification of cry1A and cry1E genes. Bioefficacy studies showed the highest mortality (50%) in the ST + FA + SD inoculation method, followed by ST (40%) in PVH27I. Moreover, growth inhibition was observed in survived larvae on inoculated plants vis-a-vis control.

Conclusion

Establishment of Bt strain as an endophyte in maize plants, complemented with insecticidal activity, could possibly lead to an innovative approach to the management of S. frugiperda and other borers.
Psychopathology of antisocial personality disorder: from the structural, functional and biochemical perspectives
Authors: Wong Rebecca Shin-Yee
Published: August 2023

Background

Antisocial personality disorder (ASPD) is characterized by a lack of empathy, a sense of guiltlessness and shamelessness, as well as impulsiveness. ASPD is a relatively common psychiatric condition in the general population, whereas individuals with ASPD often have substantial social impairments and a lower quality of life, especially for those who have mental comorbidities. This review gives an overview of the etiological and clinical aspects of ASPD and critically examines ASPD from the structural, functional and biochemical perspectives.

Results

Twin and family studies showed genetic predisposition in ASPD. Some candidate genes associated with ASPD include SLC6A4, COMT, 5-HTR2A, TPH1, DRD2, OXTR, CACNG8, COL25A1 and several serotonergic genes. Environmental factors like adverse childhood experience (ACE) and active empathy deficits in toddlerhood play a role in the etiology of ASPD, whereas low intelligence or attainment, a large family size, a convicted parent, a disrupted family, and a young mother are predictors of antisocial personality. Structural abnormalities involving the corpus callosum, amygdala, putamen, anterior cingulate cortex, as well as orbitofrontal- and dorsolateral frontal cortices have been identified in ASPD. Other observed structural changes include a decrease in grey matter volume, whole-brain volume, and white matter volume and thickness. In addition, functional abnormalities involving autonomic activity, prefrontal functions, as well as brain functional networks like sensorimotor networks, cognitive networks and cortico-striatal connectivity have been reported. Biochemical factors associated with ASPD include fatty acid amide hydrolase (FAAH) reduction in the amygdala, as well as changes in plasma levels of inflammatory biomarkers and neurotropic factors [namely, tumor necrosis factor (TNF)-α, interleukin 10 (IL-10), transforming growth factor (TGF)-β1 and brain-derived neurotrophic factor (BNDF). Increased plasma levels of testosterone, ghrelin and cortisol and decreased levels of leptin have also been implicated in ASPD.

Conclusions

To date, there is no Food and Drug Administration (FDA) approved drugs for ASPD. Understanding the disease from different perspectives is important, as this provides insights into the underlying mechanisms of ASPD, whereas the associated biochemical markers can be used as potential diagnostic and treatment targets for ASPD.
Plasma D-dimer as a biomarker for the early classification of common acute ischemic stroke subtypes in Indonesia
Authors: Akbar Muhammad , Damayanti Fitri , Tammasse Jumraini , Bintang Andi Kurnia , Aulina Susi , Soraya Gita Vita
Published: August 2023

Background

D-dimer is a well-known marker for abnormal hemostasis in acute ischemic stroke (AIS), indicating the presence of fibrin degradation due to thrombus formation and lysis. The diagnostic performance of D-dimer for different AIS types in the Indonesian population has not been established. The aim of this study is to compare the plasma D-dimer levels in three of the most common AIS subtypes in Indonesia; the cardioembolic, large artery atherosclerosis (LAA), and small-vessel occlusion (SVO), and to determine its most optimal diagnostic performance.

Results

In this cross-sectional study, 64 subjects with confirmed AIS diagnosis at the Wahidin Sudirohusodo General Hospital Makassar between June and October 2019 were recruited. Plasma D-dimer levels were measured and grouped according to the subtype of acute ischemic stroke based on the TOAST classification. A significant difference was observed between the D-dimer levels across the three AIS subtypes, with an average D-dimer of 2.93 ± 1.7, 1.27 ± 0.81, and 0.56 ± 0.46 µg/ml in the cardioembolic, LAA, and SVO subtypes, respectively. As a marker of cardioembolic stroke, an optimal cut-off was determined to be 1.52 µg/ml, yielding a sensitivity of 84.44% (CI 71.22–92.25% and specificity of 84.21% (CI 62.43–94.48%).

Conclusion

Plasma D-dimer levels varied significantly between the cardioembolic, LAA, and SVO subtypes of AIS, with the highest D-dimer level in the cardioembolic subtypes. As a marker of cardioembolic stroke, an optimal cut-off was determined to be 1.52 µg/mL, yielding a sensitivity and specificity of 84.44% and 84.21%, respectively.
Successful management by employing situational extracorporeal membrane oxygenation strategies in a patient with acute type A aortic dissection: a case report
Authors: Suzuki Kengo , Yoshida Keisuke , Hakozaki Takahiro , Tanaka Shiori , Inoue Satoki
Published: August 2023

Background

We successfully treated a case of acute type A aortic dissection in a patient with acute inferior wall infarction as well as severe circulatory and respiratory disorders.

Case presentation

A 69-year-old woman was diagnosed with acute type A aortic dissection. She received emergency partial aortic arch replacement and coronary artery bypass grafting. After the cardiopulmonary bypass, extracorporeal membrane oxygenation (ECMO) with central cannulation was performed due to severe right heart failure and extensive alveolar hemorrhage. Since the surgery, transesophageal echocardiography was used to monitor her hemodynamic status. The positive end-expiratory pressure (PEEP) was managed based on end-expiratory transpulmonary pressure. Replacement of the ECMO circuit was required every 2–3 days due to intra-circuit thrombus, and continuous renal replacement therapy was started on postoperative day (POD) 8. On POD 13, improvement of cardiac function was observed; we therefore attempted closure of the chest and conversion to veno-venous (V-V) ECMO. However, the patient’s hemodynamics were unstable due to diastolic impairment after the chest closure; thus, peripheral veno-arteriovenous (V-AV) ECMO was introduced. The patient was converted to V-V ECMO on POD 16 and weaned from ECMO on POD 17. The patient was extubated on POD 19. She left the intensive care unit with non-invasive ventilation on POD 20.

Conclusions

The favorable outcome in the current case can be attributed to the following three points: (1) appropriate ECMO strategies were employed according to the patient’s condition, (2) the patient’s lung condition improved due to transpulmonary pressure monitoring and fluid balance management from an early stage, and (3) we observed respiratory and hemodynamic status during the 50–90-s circulatory arrest periods that occurred during ECMO circuit changes, and this observation contributed to the evaluation of weaning from ECMO.
Diagnostic accuracy of B-mode, Doppler ultrasound, strain elastography, and 2D shear wave elastography in differentiation between benign and malignant lymphadenopathy
Authors: Abd-Elmageed Mohamed Kamel , Ibrahim Sahar Gamal , Zawawi Mohammed Salah Eldeen El
Published: August 2023

Background

Conventional ultrasound is used as an imaging modality for differentiation between benign and malignant lymph nodes, but with different accuracy, sensitivity, and specificity between different studies. A biopsy is the gold standard diagnosis modality but has a drawback due to its invasive nature. In general, metastatic lymph nodes present with a higher stiffness than benign lymph nodes, so ultrasound elastography could be used as an imaging modality for lymphadenopathy differentiation based on its ability to assess tissue stiffness. However, more studies should be done to assess its accuracy. Therefore, we aimed to assess the accuracy of B-mode, Doppler ultrasound, strain elastography, and 2D shear wave elastography criteria in differentiation between benign from malignant lymph nodes.

Methods

A cross-sectional study included 60 patients with lymphadenopathy. B-mode, Doppler ultrasound, strain elastography, and 2D shear wave elastography criteria were assessed for accuracy, sensitivity, and specificity compared to the final histopathological diagnosis, whether by core biopsy, fine needle aspiration, or excision biopsy.

Results

We assessed 60 patients in our study (20 benign and 40 malignant). Of these ultrasound criteria that achieved statistical significance, the highest accuracy was for elasticity ratio 88.9%, followed by strain ratio 83.8%. Also, the highest specificity was for an elasticity ratio of 80%, followed by strain score and strain ratio of 75% and 70%, respectively. Cortical asymmetry achieved the highest sensitivity, 100%, followed by strain ratio and elasticity ratio, with a sensitivity of 90% and 87.5%, respectively. However, cortical asymmetry had an extremely low specificity of 25%.

Conclusions

Ultrasound elastography criteria achieved higher accuracy compared to conventional ultrasound. So it can be used as an effective imaging modality to differentiate between benign and malignant lymph nodes.
Simultaneous spectrophotometric determination of recombined sofosbuvir, ledipasvir and paracetamol together as commonly repurposed drugs for COVID-19 treatment
Authors: Gamal Sherif , Mandour Asmaa A. , Mohamed Gehad G. , Salih Said A. , Ahmed Dina A.
Published: August 2023

Background

COVID-19 has emerged as the most serious outbreak in recent years. Certain medications such as sofosbuvir (SOF), ledipasvir (LDV) and paracetamol (PAR) were proposed as a safer and recommended substance to control symptoms of COVID-19.

Results

Using built-in spectrophotometer software, zero order and derivative spectra of the studied components, two extremely clear, quick, and sensitive spectrophotometric techniques for simultaneous determinations of SOF, LDV, as well as PAR have been developed. LDV was calculated using a zero order absorption spectrum at wavelength maxima of 333 nm. SOF and PAR were evaluated simultaneously using a first derivative spectrophotometer at 247.2 nm and 260.8 nm, respectively. The calibration graphs for SOF, LDV, and PAR are linear over ranges of concentrations of 8–60 µg/mL, 4–22 µg/mL, and 2–14 µg/mL, consequently. The suggested methodologyspecificity was investigated using laboratory manufactured (different ratios) mixtures, which were then effectively used to the analysis of Mpiviropack plus® and Panadol® pills. Valid limitations included accuracy, precision, and specificity. The methodologies were validated in accordance with some ICH standards.

Conclusions

The methods proposed were simple, accurate, precise, and neither require any complex equipment nor specific software.
A novel approach to address source overprint and shadow effects in controlled-source audio-frequency magnetotelluric exploration
Authors: Wang Shengtao , Lin Changhong
Published: August 2023

Source overprint and shadow effects can significantly affect controlled-source audio-frequency magnetotelluric (CSAMT) data and mask subsurface geologic structures; however, few solutions have been presented. Based on the traditional observation approach, we develop a new approach to solve this problem. An additional transmitter and measurement zone are introduced in the novel approach to constrain the anomalous body causing source overprint or shadow effects in the inversion. To test this approach, six experimental models are designed. Three-dimensional CSAMT responses on the six synthetic models have been simulated, and the results indicate that source overprint and shadow effects have a strong influence on the data. Strong source overprint and shadow effects are found at low frequencies. The distribution of the source overprint or shadow distortions is affected by the shape of the anomalies below the transmitter or between the transmitter and receiver. We evaluate our approach by comparing the inversion results obtained with the new and traditional approaches based on the six synthetic data sets. The results indicate that the new approach not only effectively resolves the anomalies causing source overprint or shadow distortions but also yields more accurate target results in terms of resistivity value, structure geometry, and position than the conventional scheme. The inversion of field data from Yanqing, China, further verifies the effectiveness of our approach. Compared with the traditional inversion, the new scheme inversion obtains results that are more consistent with the rock type revealed by a drillhole.
Curating “Comfort Women”
Authors: Li Lin
Published: August 2023

Using the Liji Alley Museum as a vantage point, this article examines the politics of remembering “comfort women” in contemporary China. I situate the museum within three contexts: Chinese museums on Japanese aggression, the global initiative to create women's museums, and the trans-Asian movement to found museums dealing exclusively with the comfort women issue. On the one hand, I discuss how the Liji Alley Museum has made important interventions by foregrounding the relevance of gender to discussions of civilian wartime experience and by normalizing critical discussion of violence against women. By examining the museum's physical layout, narrative frameworks, and display techniques, I reveal its use of a combination of survivors' testimonies and affective devices to engage visitors. On the other hand, I analyze its limitations vis-à-vis present-day China's gender politics. The museum's reliance on government funding and its incorporation into the state-sponsored system of patriotic education have constricted its potential to offer a more complex critique of the multilayered causes behind comfort women victims' suffering, and to connect the comfort women issue with contemporary discussions of gender-based violence.
Deep Brain Stimulation Lead Localization Variability Comparing Intraoperative MRI Versus Postoperative Computed Tomography
Authors: Yearley Alexander G. , Chua Melissa , Horn Andreas , Cosgrove G. Rees , Rolston John D.
Published: August 2023

BACKGROUND AND OBJECTIVES:

Commercially available lead localization software for deep brain stimulation (DBS) often relies on postoperative computed tomography (CT) scans to define electrode positions. When cases are performed with intraoperative MRI, another imaging set exists with which to perform these localizations. To compare DBS localization error between postoperative CT scans and intraoperative MRI.

METHODS:

A retrospective cohort of patients who underwent MRI-guided placement of DBS electrodes using the ClearPoint platform was identified. Using Brainlab Elements, postoperative CT scans were coregistered to intraoperative magnetic resonance images visualizing the ClearPoint guidance sheaths and ceramic stylets. DBS electrodes were identified in CT scans using Brainlab's lead localization tool. Trajectory and vector errors were quantified between scans for each lead in each patient.

RESULTS:

Eighty patients with a total of 157 implanted DBS electrodes were included. We observed mean trajectory and vector errors of 0.78 ± 0.44 mm (range 0.1-2.0 mm) and 1.57 ± 0.79 mm (range 0.2-4.2 mm), respectively, between postoperative CT and intraoperative MRI. There were 7 patients with CT scans collected at multiple time points. Trajectory error increased by 0.15 ± 0.42 mm (P = .31), and vector error increased by 0.22 ± 0.53 mm (P = .13) in the later scans. Across all scans, there was no significant association between trajectory (P = .053) or vector (P = .98) error and the date of CT acquisition. DBS electrodes targeting the subthalamic nucleus had significantly greater trajectory errors (P = .02) than those targeting the globus pallidus pars internus nucleus.

CONCLUSION:

Commercially available software produced largely concordant lead localizations when comparing intraoperative MRIs with postoperative CT scans, with trajectory errors on average <1 mm. CT scans tend to be more comparable with intraoperative MRI in the immediate postoperative period, with increased time intervals associated with a greater magnitude of error between modalities.