bluuebunny/crossref_metadata_2025 huggingface dataset:
Untangling the Influence of Heat Stress on Crop Phenology, Seed Set, Seed Weight, and Germination in Field Pea (Pisum sativum L.)
Authors: Lamichaney Amrit , Parihar Ashok K. , Hazra Kali K. , Dixit Girish P. , Katiyar Pradip K. , Singh Deepak , Singh Anil K. , Kumar Nitin , Singh Narendra P.
Published: March 2021

The apparent climatic extremes affect the growth and developmental process of cool-season grain legumes, especially the high-temperature stress. The present study aimed to investigate the impacts of high-temperature stress on crop phenology, seed set, and seed quality parameters, which are still uncertain in tropical environments. Therefore, a panel of 150 field pea genotypes, grouped as early (n = 88) and late (n = 62) maturing, were exposed to high-temperature environments following staggered sowing [normal sowing time or non-heat stress environment (NHSE); moderately late sowing (15 days after normal sowing) or heat stress environment-I (HSE-I); and very-late sowing (30 days after normal sowing) or HSE-II]. The average maximum temperature during flowering was about 22.5 ± 0.17°C for NHSE and increased to 25.9 ± 0.11°C and 30.6 ± 0.19°C in HSE-I and HSE-II, respectively. The average maximum temperature during the reproductive period (RP) (flowering to maturity) was in the order HSE-II (33.3 ± 0.03°C) > HSE-I (30.5 ± 0.10°C) > NHSE (27.3 ± 0.10°C). The high-temperature stress reduced the seed yield (24–60%) and seed germination (4–8%) with a prominent effect on long-duration genotypes. The maximum reduction in seed germination (>15%) was observed in HSE-II for genotypes with >115 days maturity duration, which was primarily attributed to higher ambient maximum temperature during the RP. Under HSEs, the reduction in the RP in early- and late-maturing genotypes was 13–23 and 18–33%, suggesting forced maturity for long-duration genotypes under late-sown conditions. The cumulative growing degree days at different crop stages had significant associations (p < 0.001) with seed germination in both early- and late-maturing genotypes; and the results further demonstrate that an extended vegetative period could enhance the 100-seed weight and seed germination. Reduction in seed set (7–14%) and 100-seed weight (6–16%) was observed under HSEs, particularly in HSE-II. The positive associations of 100-seed weight were observed with seed germination and germination rate in the late-maturing genotypes, whereas in early-maturing genotypes, a negative association was observed for 100-seed weight and germination rate. The GGE biplot analysis identified IPFD 11-5, Pant P-72, P-1544-1, and HUDP 11 as superior genotypes, as they possess an ability to produce more viable seeds under heat stress conditions. Such genotypes will be useful in developing field pea varieties for quality seed production under the high-temperature environments.
Rasch Analysis of Authentic Evaluation of Young Children's Functioning in Classroom Routines
Authors: Morales-Murillo Catalina Patricia , García-Grau Pau , McWilliam R. A. , Grau Sevilla Ma Dolores
Published: March 2021

This study evaluated the functioning of children in early childhood education classroom routines, using the 3M Functioning in Preschool Routines Scale. A total of 366 children aged 36 to 70 months and 22 teachers from six early childhood education centers in Spain participated in the study. The authors used the Rasch model to determine the item fit and the difficulty of the items in relation to children's ability levels in this age range. The Rasch Differential Item Functioning (DIF) analysis by child age groups showed that the item difficulty differed according to the children's age and according to their levels of competence. The results of this study supported the reliability and validity of the 3M scale for assessing children's functioning in preschool classroom routines. A few items, however, were identified as needing to be reworded and more difficult items needed to be added to increase the scale difficulty level to match the performance of children with higher ability levels. The authors introduced the new and reworded items based on the results of this study and the corresponding ICF codes per item. Moreover, the authors indicate how to use the ICF Performance Qualifiers in relation to the 3M scale response categories for developing a functioning profile for the child.
The Indirect Effect of Trauma via Cognitive Biases and Self-Disturbances on Psychotic-Like Experiences
Authors: Pionke-Ubych Renata , Frydecka Dorota , Cechnicki Andrzej , Nelson Barnaby , Gawęda Łukasz
Published: March 2021

Although self-disturbances (SD) are considered to be a core psychopathological feature of schizophrenia spectrum disorders, there is still insufficient empirical data on the mechanisms underlying these anomalous self-experiences. The aim of the present study was to test a hypothesized model in which cognitive biases and exposure to traumatic life events are related to the frequency of SD which, in turn, contribute to the frequency of psychotic-like experiences (PLEs). Our sample consisted of 193 Polish young adults from the general population (111 females; 18–35 years of age, M = 25.36, SD = 4.69) who experience frequent PLEs. Participants were interviewed for PLEs, SD and social functioning as well as completed self-reported questionnaires and behavioral tasks that measure cognitive biases (e.g., safety behaviors, attention to threat, external attribution, jumping to conclusion, source monitoring, overperceptualization). The model was tested using path analysis with structural equation modeling. All of the hypothesized relationships were statistically significant and our model fit the data well [χ2(23) = 31.201;p= 0.118; RMSEA = 0.043 (90% CI = 0.00–0.078), CFI = 0.985, SRMR = 0.041, TLI = 0.976]. The results revealed a significant indirect effect of traumatic life events on PLEs through SD and self-reported cognitive biases. However, performance-based cognitive biases measured with three behavioral tasks were unrelated to SD and PLEs. The frequency of SD explained a substantial part (43.1%) of the variance in PLEs. Further studies with longitudinal designs and clinical samples are required to verify the predictive value of the model.
The Epidemiology of COVID 19 in the Amazon and the Guianas: Similarities, Differences, and International Comparisons
Authors: Nacher Mathieu , Rousseau Cyril , Succo Tiphanie , Andrieu Audrey , Gaillet Mélanie , Michaud Céline , Servas Véronique , Douine Maylis , Schaub Roxane , Adenis Antoine , Demar Magalie , Abboud Philippe , Epelboin Loïc , Djossou Félix
Published: March 2021

Background: The COVID 19 epidemic submerged many health systems in the Amazon. The objective of the present study was to focus on the epidemic curves of the COVID 19 epidemic in different centers, and to look at testing and mortality data.

Methods: Publicly available datasets were used. The log10 of the daily cumulated number of cases starting from the day the territory reached 100 cumulated cases was plotted to compare the magnitude, shape and slope of the different curves. The maximum daily testing efforts were plotted for each territory in relation to the maximum daily number of diagnoses. The case fatality rate was computed by dividing the number of COVID 19 deaths by the number of confirmed cases.

Results: In the Amazonian regions in general the speed of growth was generally lower than in Europe or the USA, or Southern Brazil. Whereas, countries like South Korea or New Zealand “broke” the curve relatively rapidly the log linear trajectory seemed much longer with signs of a decline in growth rate as of early July 2020. After a very slow start, French Guiana had the lowest slope when compared to other Amazonian territories with significant epidemics. The Amazonian states of Roraima, Amazonas, Parà, and Amapà had among the highest number of cases and deaths per million inhabitants in the world. French Guiana had significantly fewer deaths relative to its number of confirmed cases than other Amazonian territories. French Guiana had a late epidemic surge with intense testing scale-up often exceeding 4,000 persons tested daily per million inhabitants. Brazil was an outlier with low daily testing levels in relation to the number of daily diagnoses.

Conclusions: There were marked heterogeneities mortality rates suggesting that socioeconomic, political factors, and perhaps ethnic vulnerability led to striking outcome differences in this Amazonian context.
Determinants of Infodemics During Disease Outbreaks: A Systematic Review
Authors: Alvarez-Galvez Javier , Suarez-Lledo Victor , Rojas-Garcia Antonio
Published: March 2021

Background: The widespread use of social media represents an unprecedented opportunity for health promotion. We have more information and evidence-based health related knowledge, for instance about healthy habits or possible risk behaviors. However, these tools also carry some disadvantages since they also open the door to new social and health risks, in particular during health emergencies. This systematic review aims to study the determinants of infodemics during disease outbreaks, drawing on both quantitative and qualitative methods.

Methods: We searched research articles in PubMed, Scopus, Medline, Embase, CINAHL, Sociological abstracts, Cochrane Library, and Web of Science. Additional research works were included by searching bibliographies of electronically retrieved review articles.

Results: Finally, 42 studies were included in the review. Five determinants of infodemics were identified: (1) information sources; (2) online communities' structure and consensus; (3) communication channels (i.e., mass media, social media, forums, and websites); (4) messages content (i.e., quality of information, sensationalism, etc.,); and (5) context (e.g., social consensus, health emergencies, public opinion, etc.). Studied selected in this systematic review identified different measures to combat misinformation during outbreaks.

Conclusion: The clarity of the health promotion messages has been proven essential to prevent the spread of a particular disease and to avoid potential risks, but it is also fundamental to understand the network structure of social media platforms and the emergency context where misinformation might dynamically evolve. Therefore, in order to prevent future infodemics, special attention will need to be paid both to increase the visibility of evidence-based knowledge generated by health organizations and academia, and to detect the possible sources of mis/disinformation.
Online Survey of the Impact of COVID-19 Risk and Cost Estimates on Worry and Health Behavior Compliance in Young Adults
Authors: Imbriano Gabriella , Larsen Emmett M. , Mackin Daniel M. , An Akaisha Kaixuan , Luhmann Christian C. , Mohanty Aprajita , Jin Jingwen
Published: March 2021

The novel coronavirus COVID-19 pandemic is associated with elevated rates of anxiety and relatively lower compliance with public health guidelines in younger adults. To develop strategies for reducing anxiety and increasing adherence with health guidelines, it is important to understand the factors that contribute to anxiety and health compliance in the context of COVID-19. Earlier research has shown that greater perceived risk of negative events and their costs are associated with increased anxiety and compliance with health behaviors, but it is unclear what role they play in a novel pandemic surrounded by uncertainty. In the present study we measured (1) perceived risk as the self-reported probability of being infected and experiencing serious symptoms due to COVID-19 and (2) perceived cost as financial, real-world, physical, social, and emotional consequences of being infected with COVID-19. Worry was assessed using the Penn State Worry Questionnaire (PWSQ) and health compliance was measured as endorsement of the World Health Organization (WHO) health directives for COVID-19. Our results showed that greater perceived risk and costs of contracting the COVID-19 virus were associated with greater worry and while only costs were associated with greater compliance with health behaviors. Neither self-reported worry nor its interaction with cost estimates was associated with increased engagement in health behaviors. Our results provide important insight into decision making mechanisms involved in both increased anxiety and health compliance in COVID-19 and have implications for developing psychoeducational and psychotherapeutic strategies to target both domains.
What Does Social Support Sound Like? Challenges and Opportunities for Using Passive Episodic Audio Collection to Assess the Social Environment
Authors: Poudyal Anubhuti , van Heerden Alastair , Hagaman Ashley , Islam Celia , Thapa Ada , Maharjan Sujen Man , Byanjankar Prabin , Kohrt Brandon A.
Published: March 2021

Background: The social environment, comprised of social support, social burden, and quality of interactions, influences a range of health outcomes, including mental health. Passive audio data collection on mobile phones (e.g., episodic recording of the auditory environment without requiring any active input from the phone user) enables new opportunities to understand the social environment. We evaluated the use of passive audio collection on mobile phones as a window into the social environment while conducting a study of mental health among adolescent and young mothers in Nepal.

Methods: We enrolled 23 adolescent and young mothers who first participated in qualitative interviews to describe their social support and identify sounds potentially associated with that support. Then, episodic recordings were collected for 2 weeks from the mothers using an app to record 30 s of audio every 15 min from 4 A.M. to 9 P.M. Audio data were processed and classified using a pretrained model. Each classification category was accompanied by an estimated accuracy score. Manual validation of the machine-predicted speech and non-speech categories was done for accuracy.

Results: In qualitative interviews, mothers described a range of positive and negative social interactions and the sounds that accompanied these. Potential positive sounds included adult speech and laughter, infant babbling and laughter, and sounds from baby toys. Sounds characterizing negative stimuli included yelling, crying, screaming by adults and crying by infants. Sounds associated with social isolation included silence and TV or radio noises. Speech comprised 43% of all passively recorded audio clips (n = 7,725). Manual validation showed a 23% false positive rate and 62% false-negative rate for speech, demonstrating potential underestimation of speech exposure. Other common sounds were music and vehicular noises.

Conclusions: Passively capturing audio has the potential to improve understanding of the social environment. However, a pre-trained model had the limited accuracy for identifying speech and lacked categories allowing distinction between positive and negative social interactions. To improve the contribution of passive audio collection to understanding the social environment, future work should improve the accuracy of audio categorization, code for constellations of sounds, and combine audio with other smartphone data collection such as location and activity.
Factors Associated With Positive Self-Rated Health: Comparing Older Adults in Brazil and in Portugal
Authors: Cachioni Meire , Cipolli Gabriela Cabett , Borim Flávia Silva Arbex , Batistoni Samila Sathler Tavares , Yassuda Mônica Sanches , Neri Anita Liberalesso , Paúl Constança
Published: March 2021

Introduction: Self-rated health is a multidimensional health indicator and a predictor of adverse events in old age. Answers to this assessment are influenced by social, cultural and personality factors.

Aim: Exploring common and distinctive characteristics of Brazilian and Portuguese older adults aged 70 and over regarding positive self-rated health according to sociodemographic variables, to functional capacity, to independent performance of basic activities of daily living and to neuroticism, as well as analyzing associations between positive self-rated health and these variables.

Methods: The present paper is a comparative and cross-sectional study based on secondary data contained in the databases of the FIBRA (Frailty in Brazilian Older Adults) follow-up study, with 418 Brazilian older adults, and of the DIA (From Disability to Activity: The Challenge of Aging) study, with 380 Portuguese older adults. Both samples had higher percentages of women: 68.4% for Portugal and 69.9% for Brazil. The Brazilian sample had a higher average age (80.31 ± 4.67) than the Portuguese sample (76.80 ± 5.28).

Results: The Portuguese older adults had better overall cognition scores, higher handgrip strength and higher neuroticism values than the Brazilian older adults. In the simple and multiple logistic regression analyses, it was found that among Brazilian older adults, subjects with higher scores in the MMSE (OR 1.16; 95% CI 1.08–1.24), regardless of ADL performance (OR 2.13; 95% CI 1.31–3.47) and with scores 24–29 (OR 1.92; 95% CI 1.07–3.43) or 11–23 (OR 2.09; 95% CI 1.15–3.79) in neuroticism were more likely to assess their health as very good/good. On the other hand, the Portuguese older adults with intermediate 24–9 (OR 2.38; 95% CI 1.31–4.33) or low 11–23 (OR 5.31; 95% CI 2.69–10.45) scores in neuroticism were more likely to evaluate their health as very good/good.

Conclusion: Based on the findings of the present study and on the existing literature, it may be said that it is possible for people to age while keeping a positive perception of their own health, even in advanced old age; comparisons between the above-mentioned countries, however, point to the need for investments in healthcare systems so that older adults may enjoy greater physical independence and improved mental health.
Identity Multiplicity in an Ethnic and Religious Minority in Latvia: Old Believer Youth
Authors: Stasulane Anita
Published: March 2021

The paper explores the relationship between the religious identity and the ethnic and national identities of Old Believer youth in Latvia. This case is of particular interest in providing an in-depth insight into the intersection of ethnicity, nationality and religion, as the Old Believers are an ethnic and religious minority living in Latvia. Applying the concepts of multiple identities, this article explores the role of religion played in the integration of identity among young people belonging to the Old Believer religious community: their self-understanding as a composition of intersecting identities that influence each other; the manifestations of the intersection of various identities; the relationship of identity integration to religion. The analysis is based on the findings of ethnographic research conducted in the Old Believer youth group in Daugavpils (Latvia) within a framework of the international project “Cultural Heritage and Identities of Europe’s Future”, funded from the European Union’s Horizon 2020 Research and Innovation Program under Grant Agreement No. 770464. The author has highlighted that today’s rapid changes are leading to identity crisis: an individual faces difficulty in shaping and maintaining a stable identity, since economic life is becoming increasingly unpredictable and communities are becoming fragmented. The identity of Latvian Old Believer youth forms and develops in a local cultural context, and is affected by the social change. The qualitative data collected during the fieldwork provided a useful resource for an analysis of belonging, the crucial factor in the formation of identity for Old Believer youth. As the voices of young people in this study reveal, three types of belonging characterize Old Believer youth: their ethnicity, which interacts with national belonging in a complex way; the local belonging, which is stronger than the global one; the European belonging, which conflicts with national belonging and ethnicity.
Telework and Lifelong Learning
Authors: Bjursell Cecilia , Bergmo-Prvulovic Ingela , Hedegaard Joel
Published: March 2021

The increase of telework during the pandemic is predicted to impact working life, not only in terms of a larger number of employees working from home, but more importantly, it may transform the way we conceptualise work. This will in turn impact systems for and participation in lifelong learning. There is a risk for increased social inequalities, as neither telework nor lifelong learning is evenly distributed among workers. Statistics on telework in the EU show that there are differences between age groups, nations, sectors, and professions. If these trends will steer forward, there is a risk of widening gaps between countries, companies, and workers. To establish the current knowledge base, we have gathered literature reviews from several disciplines. One finding is that the previous literature on telework has not included lifelong learning in any form (formal, non-formal and informal). Based on a review of previous studies, we suggest a number of research questions for future research. This is relevant as research about telework and lifelong learning has the potential to contribute to a sustainable working life in terms of providing more flexible arrangements for employees and to support the lifelong learning that takes place in contexts such as the office, home, online meetings, and virtual reality.
Homogeneous vs Biased IGM: Impact on Reionization
Authors: Raut Dinesh
Published: March 2021

This paper considers the impact of large scale biasing of the IGM on reionization. The two simplest but extreme scenarios for IGM biasing are: an unbiased IGM which has a constant density and an IGM with density equal to the collapsed matter density. In this work, the relationship between the IGM density and the collapsed matter density is defined through an IGM bias parameter. The two extreme scenarios of homogeneous and perfectly biased IGM are produced for two extreme values of this bias parameter. It is found that, for the same level of reionization (i.e., for same global neutral hydrogen fraction). one could get very different 21 cm brightness temperature distributions for different values of this bias parameter. These distributions could give an order of magnitude more or less power as compared to the uniform case. It is also found that there exists a critical value for the IGM bias parameter for which there could be a near washout of the structure in the 21 cm brightness temperature distribution (i.e., zero power or a nearly uniform 21 cm brightness temperature distribution). To address the problem, a new method of generating 21 cm brightness temperature maps is used. The method uses the results of n-body simulations and then employs ray tracing to obtain the 21 cm brightness temperature maps. Towards the end, a prescription for the IGM bias parameter is given. This is derived within the framework of the Press-Schechter theory.
Evaluation of Three Medetomidine-Based Anesthetic Protocols in Free-Ranging Wild Boars (Sus scrofa)
Authors: Morelli Jacopo , Rossi Sophie , Fuchs Boris , Richard Emmanuelle , Barros Daniela S. B. , Küker Susanne , Arnemo Jon M. , Evans Alina L.
Published: March 2021

Three medetomidine-based drug protocols were compared by evaluating time courses, reliability and physiological effects in wild boars. A total of 21 cage-trapped wild boars (Sus scrofa) were immobilized using one of the following drug combinations; MTZ: medetomidine (0.2 mg/kg) + tiletamine-zolazepam (2.0 mg/kg), MK: medetomidine (0.15 mg/kg) + ketamine (5 mg/kg), and MKB: medetomidine (0.1 mg/kg) + ketamine (5.0 mg/kg) + butorphanol (0.2 mg/kg). Induction time, recovery time, and physiological variables were recorded and arterial blood gas analysis measured twice, before and after 15 min of oxygen supplementation (0.5–1.0 L/min). For reversal, 4 mg of atipamezole per mg of medetomidine was administered intramuscularly. The boars recovered in the cage and were released once ataxia resolved. The MK group had significantly longer recovery times (mean 164 min ± 79 SD) compared to the other groups. MKB elicited longer and incomplete induction compared to the other groups (mean induction time 20 min ± 10 SD), decreasing the efficiency of the capture and increasing the risk of hyperthermia. Both ketamine-based protocols required additional ketamine intramuscularly to prolong the anesthesia after 20–40 min from induction. Agreement between the pulse oximeter and the blood gas analyzer was low, with the pulse oximeter underestimating the real values of arterial oxyhemoglobin saturation, particularly at higher readings. Mild acute respiratory acidosis (PaCO245–60 mmHg) and mild to moderate hypoxemia (PaO269–80 mmHg) occurred in most boars, regardless of the treatment group but especially in the MKB group. The acid-base status improved and hypoxemia resolved in all boars during oxygen supplementation, with the PaO2rising above the physiological reference range (81.6–107.7 mmHg) in many individuals. MK and MKB induced safe and reliable immobilization of wild boars for at least 20 min. Supplemental oxygen delivery is recommended in order to prevent hypoxemia in wild boars immobilized with the protocols used in the present study. Long and ataxic recoveries occurred in most animals, regardless of the protocol, but especially in the MKB group.
The Association of Family-Related Adversity With Fighting in Adolescents: Does Hopelessness Mediate This Association?
Authors: Macková Jaroslava , Dankulincova Veselska Zuzana , Madarasova Geckova Andrea , Jansen Danielle , van Dijk Jitse P. , Reijneveld Sijmen A.
Published: March 2021

Objectives: To explore the association of family-related adversities with physical fighting, and whether this association is mediated by hopelessness.

Methods: The sample consisted of 3712 Slovak adolescents (mean age: 13.9, 50.7% girls). Participants answered questions regarding experienced family-related adversities, involvement in physical fighting in the last 12 months and the Hopelessness Questionnaire. First, the association of family adversities in general with fighting and of each of family-related adversity separately was assessed using linear regression models and second, mediation was assessed using the a*b product method with bootstrapped 95% confidence intervals

Results: Adolescents who had experienced at least one family adversity reported more frequent fighting. Similarly, each of reported family adversities (death of a parent, substance abuse problems of a parent, conflicts/physical fights, divorce) was associated with more frequent fighting among adolescents. The mediation effect of hopelessness was found in each association of family-related adversity with fighting.

Conclusion: These findings suggest that interventions to support adolescents who had experienced family adversities could among other things be directed at better coping with hopelessness.
El trastorno de personalidad por evitación. De la psicoterapia descriptiva a un modelo de mantenimiento del trastorno.
Authors: Procacci Michele , Dimaggio Giancarlo , Semerari Antonio
Published: March 2021

El evitante se describe como el trastorno de personalidad más sensible al fracaso en el establecimiento de relaciones. A partir de las dimensiones mentales que lo componen (déficits metacognitivos, sentido de no pertenencia, estados mentales disfuncionales) se intenta proponer un modelo psicopatológico que vaya más allá de la mera descripción de síntomas propuesta por el DSM IV. Asimismo, se plantea la posibilidad de que existan mecanismos que favorezcan el mantenimiento de las partes básicas del trastorno. En este sentido, la descripción de los ciclos interpersonales disfuncionales más característicos también aporta elementos que permiten comprender las dificultades relacionales que se presentan al iniciar una psicoterapia con estos individuos. Finalmente, se propone un modelo circular del trastorno útil para poner en marcha estrategias específicas en el tratamiento cognitivista del Trastorno de Personalidad por Evitación.
Regional Differences in Benefits from the EU Common Agricultural Policy in Poland and Their Policy Implications
Authors: Kiryluk-Dryjska Ewa , Baer-Nawrocka Agnieszka
Published: March 2021

Although the beneficial impact of Common Agricultural Policy (CAP) on the stabilization on farming income is undisputable, the distribution of benefits derived from the CAP between operators and regions gives rise to some controversy. The objective of this paper was to estimate regional differences in the benefits derived from the Common Agricultural Policy in Poland based on the partial equilibrium model of the European Union (EU) agricultural sector with simulated interventions. The expectations of farmers from different regions of Poland were represented as a non-cooperative game to define vectors of change in the agricultural policy. The theory of moves was applied to set the game between different groups of farmers. Our results demonstrate that both the 1st and the 2nd pillars of the CAP were more profitable to farmers from regions with a more advantageous agrarian structure and a higher agricultural potential compared to their peers from the regions with a fragmented agriculture. However, considering long-term development objectives of the Polish agriculture, the theory of moves outcome argues against compensating for these differences by increasing redistributive payments to farmers in less favorable regions. To prevent widening of regional differences and ensure the social and economic development of rural areas in regions with less favorable agrarian structures where agriculture is currently unable to compete, it would be critical to enhance conditions for alternative types of economic activities.
Extracts of Emmer Wheatgrass Grown with Distilled Water, Salinity or Selenium Differently Affect Germination and Cytosolic Ca2+ of Maize Pollen
Authors: Del Pino Alberto Marco , Falcinelli Beatrice , D’Amato Roberto , Businelli Daniela , Benincasa Paolo , Palmerini Carlo Alberto
Published: March 2021

In this work, the biological activity of emmer (Triticum turgidum L. spp. dicoccum (Schrank ex Shubler) Thell.) wheatgrass extracts obtained from grains sprouted with distilled water, or salinity (50 mM) or selenium (45 mg L−1 of Na2SeO3), was tested through an experimental biological model based on the germination and cytosolic Ca2+ homeostasis of maize pollen grains. The effects of thapsigargin (TG) and of four phenolic acids (PAs: ferulic, coumaric, salicylic and 3-HO benzoic) on maize pollen were also tested as controls. Wheatgrass extracts influenced both pollen cytosolic Ca2+ and germination. The Ca2+ agonist activity of emmer wheatgrass was transient, different from that of TG, which caused a depletion of the stored Ca2+ and a permanent alteration of Ca2+ homeostasis. The results obtained with extracts compared to those obtained with pure PAs suggest that PAs in unconjugated forms, which are known to be well represent in emmer wheatgrass, contribute to the biological activity of extracts. The extent of the biological response of emmer wheatgrass extracts was influenced by emmer sprouting conditions (i.e., distilled water, or salinity or selenium). Maize pollen treated with Se-enriched wheatgrass extracts showed a less perturbed cytosolic Ca2+ and a higher germination rate.
Yield and Quality of Inflorescences in the Zantedeschia albomaculata (Hook.) Baill. ‘Albomaculata’ after the Treatment with AMF and GA3
Authors: Andrzejak Roman , Janowska Beata
Published: March 2021

This study was conducted to assess the influence of gibberellic acid (GA3) and arbuscular mycorrhizal fungi (AMF) on the flowering and quality of Zantedeschia albomaculata (Hook.) Baill ‘Albomaculata’ plants. Before planting, the rhizomes were soaked in water or an aqueous solution of GA3 at a concentration of 150 mg dm−3 for 30 min. A mixture of AMF was applied to the rhizomes a week after planting. The AMF treatment increased the yield of inflorescences of the ‘Albomaculata’ cultivar by 100%. AMF and GA3 had a favourable effect on the quality of inflorescences, expressed by the length of peduncles, whereas AMF individually positively affected the length of the spathes. AMF and GA3 had no effect on the level of macroelements in calla lily leaves, with the exception of calcium (Ca). The leaves of mycorrhized plants had a high content of sodium (Na) and micronutrients, except for iron (Fe). The results of the study showed that GA3 could be replaced by mycorrhizal inoculation when applied to Zantedeschia plants.
New Autoantibody Specificities in Systemic Sclerosis and Very Early Systemic Sclerosis
Authors: Lande Roberto , Palazzo Raffaella , Mennella Anna , Pietraforte Immacolata , Cadar Marius , Stefanantoni Katia , Conrad Curdin , Riccieri Valeria , Frasca Loredana
Published: March 2021

Chemokine (C-X-C motif) ligand 4 (CXCL4) is a biomarker of unfavorable prognosis in Systemic Sclerosis (SSc), a potentially severe autoimmune condition, characterized by vasculitis, fibrosis and interferon (IFN)-I-signature. We recently reported that autoantibodies to CXCL4 circulate in SSc patients and correlate with IFN-α. Here, we used shorter versions of CXCL4 and CXCL4-L1, the CXCL4 non-allelic variant, to search for autoantibodies exclusively reacting to one or the other CXCL4 form. Moreover, to address whether anti-CXCL4/CXCL4-L1 antibodies were present before SSc onset and predicted SSc-progression, we longitudinally studied two VEDOSS (Very Early Diagnosis of Systemic Sclerosis) patient cohorts, separating SSc-progressors from SSc-non-progressors. We found that anti-CXCL4-specific autoantibodies were present in both SSc and VEDOSS patients (both SSc-progressors and SSc-non-progressors). Anti-CXCL4-L1-specific autoantibodies were especially detected in long-standing SSc (lsSSc). Anti-CXCL4/CXCL4-L1 antibodies correlated with IFN-α and with specific SSc-skin features but only in lsSSc and not in early SSc (eaSSc) or VEDOSS. Thus, a broader antibody response, with reactivity spreading to CXCL4-L1, is characteristic of lsSSc. The early anti-CXCL4 autoantibody response seems qualitatively different from, and likely less pathogenic than, that observed in advanced SSc. Lastly, we confirm that anti-CXCL4 autoantibodies are SSc-biomarkers and uncover that also CXCL4-L1 becomes an autoantigen in lsSSc.
In Vitro Synergistic Interactions of Isavuconazole and Echinocandins against Candida auris
Authors: Caballero Unai , Kim Sarah , Eraso Elena , Quindós Guillermo , Vozmediano Valvanera , Schmidt Stephan , Jauregizar Nerea
Published: March 2021

Candida auris is an emergent fungal pathogen that causes severe infectious outbreaks globally. The public health concern when dealing with this pathogen is mainly due to reduced susceptibility to current antifungal drugs. A valuable alternative to overcome this problem is to investigate the efficacy of combination therapy. The aim of this study was to determine the in vitro interactions of isavuconazole with echinocandins against C. auris. Interactions were determined using a checkerboard method, and absorbance data were analyzed with different approaches: the fractional inhibitory concentration index (FICI), Greco universal response surface approach, and Bliss interaction model. All models were in accordance and showed that combinations of isavuconazole with echinocandins resulted in an overall synergistic interaction. A wide range of concentrations within the therapeutic range were selected to perform time-kill curves. These confirmed that isavuconazole–echinocandin combinations were more effective than monotherapy regimens. Synergism and fungistatic activity were achieved with combinations that included isavuconazole in low concentrations (≥0.125 mg/L) and ≥1 mg/L of echinocandin. Time-kill curves revealed that once synergy was achieved, combinations of higher drug concentrations did not improve the antifungal activity. This work launches promising results regarding the combination of isavuconazole with echinocandins for the treatment of C. auris infections.
Allergic Diseases Caused by Aspergillus Species in Patients with Cystic Fibrosis
Authors: Curran Aidan K. , Hava David L.
Published: March 2021

Aspergillus spp. are spore forming molds; a subset of which are clinically relevant to humans and can cause significant morbidity and mortality. A. fumigatus causes chronic infection in patients with chronic lung disease such as asthma, chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF). In patients with CF, A. fumigatus infection can lead to allergic disease, such as allergic bronchopulmonary aspergillosis (ABPA) which is associated with high rates of hospitalizations for acute exacerbations and lower lung function. ABPA results from TH2 immune response to Aspergillus antigens produced during hyphal growth, marked by high levels of IgE and eosinophil activation. Clinically, patients with ABPA experience difficulty breathing; exacerbations of disease and are at high risk for bronchiectasis and lung fibrosis. Oral corticosteroids are used to manage aspects of the inflammatory response and antifungal agents are used to reduce fungal burden and lower the exposure to fungal antigens. As the appreciation for the severity of fungal infections has grown, new therapies have emerged that aim to improve treatment and outcomes for patients with CF.
Butterfly Pea Flower (Clitoria ternatea Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats
Authors: Maneesai Putcharawipa , Iampanichakul Metee , Chaihongsa Nisita , Poasakate Anuson , Potue Prapassorn , Rattanakanokchai Siwayu , Bunbupha Sarawoot , Chiangsaen Petcharat , Pakdeechote Poungrat
Published: March 2021

In this study, we examine whether Clitoria ternatea Linn. (CT) can prevent Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced cardiac and vascular dysfunction in rats. Male Sprague Dawley rats were given L-NAME (40 mg/kg, drinking water) and orally administered with CT extract (300 mg/kg/day) or lisinopril (2.5 mg/kg/day) for 5 weeks. The main phytochemical components of the CT extract were found to be flavonoids. The CT extract alleviated the high blood pressure in rats receiving L-NAME. Decreased vasorelaxation responses to acetylcholine and enhanced contractile responses to sympathetic nerve stimulation in aortic rings and mesenteric vascular beds of L-NAME treated rats were ameliorated by CT extract supplementation. Left ventricular hypertrophy and dysfunction were developed in L-NAME rats, which were partially prevented by CT extract treatment. The CT extract alleviated upregulated endothelial nitric oxide synthase expression, decreased plasma nitrate/nitrite levels, and increased oxidative stress in L-NAME rats. It suppressed high levels of serum angiotensin-converting enzyme activity, plasma angiotensin II, and cardiac angiotensin II type 1 receptor, NADPH oxidases 2, nuclear factor-kappa B, and tumor necrosis factor-alpha expression. The CT extract, therefore, partially prevented L-NAME-induced hypertension and cardiovascular alterations in rats. These effects might be related to a reduction in the oxidative stress and renin–angiotensin system activation due to L-NAME in rats.
Imaging Biomarkers for Monitoring the Inflammatory Redox Landscape in the Brain
Authors: Fernandes Eduardo Felipe Alves , Özcelik Dennis
Published: March 2021

Inflammation is one key process in driving cellular redox homeostasis toward oxidative stress, which perpetuates inflammation. In the brain, this interplay results in a vicious cycle of cell death, the loss of neurons, and leakage of the blood–brain barrier. Hence, the neuroinflammatory response fuels the development of acute and chronic inflammatory diseases. Interrogation of the interplay between inflammation, oxidative stress, and cell death in neurological tissue in vivo is very challenging. The complexity of the underlying biological process and the fragility of the brain limit our understanding of the cause and the adequate diagnostics of neuroinflammatory diseases. In recent years, advancements in the development of molecular imaging agents addressed this limitation and enabled imaging of biomarkers of neuroinflammation in the brain. Notable redox biomarkers for imaging with positron emission tomography (PET) tracers are the 18 kDa translocator protein (TSPO) and monoamine oxygenase B (MAO–B). These findings and achievements offer the opportunity for novel diagnostic applications and therapeutic strategies. This review summarizes experimental as well as established pharmaceutical and biotechnological tools for imaging the inflammatory redox landscape in the brain, and provides a glimpse into future applications.
Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis
Authors: Amara Ines , Ontario Maria Laura , Scuto Maria , Lo Dico Gianluigi Maria , Sciuto Sebastiano , Greco Valentina , Abid-Essefi Salwa , Signorile Anna , Salinaro Angela Trovato , Calabrese Vittorio
Published: March 2021

Moringa oleifera (MO) is a medicinal plant that has been shown to possess antioxidant, anticarcinogenic and antibiotic activities. In a rat model, MO extract (MOe) has been shown to have a protective effect against brain damage and memory decline. As an extending study, here, we have examined the protective effect of MOe against oxidative stress and apoptosis caused in human neuroblastome (SH-SY5Y) cells by di-(2-ethylhexyl) phthalate (DEHP), a plasticizer known to induce neurotoxicity. Our data show that MOe prevents oxidative damage by lowering reactive oxygen species (ROS) formation, restoring mitochondrial respiratory chain complex activities, and, in addition, by modulating the expression of vitagenes, i.e., antioxidant proteins Nrf2 and HO-1. Moreover, MOe prevented neuronal damage by partly inhibiting endoplasmic reticulum (ER) stress response, as indicated by decreased expression of CCAAT-enhancer-binding protein homologous protein (CHOP) and Glucose-regulated protein 78 (GRP78) proteins. MOe also protected SH-SY5Y cells from DEHP-induced apoptosis, preserving mitochondrial membrane permeability and caspase-3 activation. Our findings provide insight into understanding of molecular mechanisms involved in neuroprotective effects by MOe against DEHP damage.
Cold Crystallization Kinetics and Thermal Degradation of PLA Composites with Metal Oxide Nanofillers
Authors: Tarani Evangelia , Pušnik Črešnar Klementina , Zemljič Lidija Fras , Chrissafis Konstantinos , Papageorgiou George Z. , Lambropoulou Dimitra , Zamboulis Alexandra , N. Bikiaris Dimitrios , Terzopoulou Zoi
Published: March 2021

Poly(lactic acid) (PLA) nanocomposites with antimicrobial fillers have been increasingly explored as food packaging materials that are made of a biobased matrix and can minimize food loss due to spoilage. Some of the most commonly studied fillers are zinc oxide (ZnO), titanium dioxide (TiO2), and silver nanoparticles (AgNPs). In this work, nanocomposites with 1 wt.% of each filler were prepared by melt mixing. An extensive study of thermally stimulated processes such as crystallization, nucleation, degradation, and their kinetics was carried out using Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA). In detail, non-isothermal cold crystallization studies were performed with DSC and polarized light microscopy (PLM), and kinetics were analyzed with multiple equations. The activation energy of the non-isothermal cold crystallization was calculated with the methods of Kissinger and Friedman. The latter was used to also determine the Hoffman–Lauritzen parameters (Kg and U*) by applying the Vyazovkin method. Additionally, effective activation energy and kinetic parameters of the thermal decomposition process were determined by applying the isoconversional differential method and multivariate non-linear regression method. According to TGA results, metal oxide nanofillers affected the thermal stability of PLA and caused a decrease in the activation energy values. Moreover, the fillers acted as heterogenous nucleating agents, accelerating the non-isothermal crystallization of PLA, thus reducing its activation energy. It can be concluded that metal oxide nanofillers catalytically affect the thermal degradation and crystallization of PLA samples.
Multi-View Attention Network for Visual Dialog
Authors: Park Sungjin , Whang Taesun , Yoon Yeochan , Lim Heuiseok
Published: March 2021

Visual dialog is a challenging vision-language task in which a series of questions visually grounded by a given image are answered. To resolve the visual dialog task, a high-level understanding of various multimodal inputs (e.g., question, dialog history, and image) is required. Specifically, it is necessary for an agent to (1) determine the semantic intent of question and (2) align question-relevant textual and visual contents among heterogeneous modality inputs. In this paper, we propose Multi-View Attention Network (MVAN), which leverages multiple views about heterogeneous inputs based on attention mechanisms. MVAN effectively captures the question-relevant information from the dialog history with two complementary modules (i.e., Topic Aggregation and Context Matching), and builds multimodal representations through sequential alignment processes (i.e., Modality Alignment). Experimental results on VisDial v1.0 dataset show the effectiveness of our proposed model, which outperforms previous state-of-the-art methods under both single model and ensemble settings.
Single Output and Algebraic Modal Parameters Identification of a Wind Turbine Blade: Experimental Results
Authors: Trujillo-Franco Luis Gerardo , Abundis-Fong Hugo Francisco , Campos-Amezcua Rafael , Gomez-Martinez Roberto , Martinez-Perez Armando Irvin , Campos-Amezcua Alfonso
Published: March 2021

This paper describes the evaluation of a single output, online, and time domain modal parameters identification technique based on differential algebra and operational calculus. In addition, an analysis of the frequency response function (FRF) of the system is conducted in a specific set up, emulating its nominal or operational conditions to determine the influence of the non-linearities over the dynamic behavior of the system in those particular magnitudes of deformations; thus, this influence is quantified by a numerical index. This methodology is applied to a wind turbine blade submitted to wind tunnel experiments. The natural frequencies and modal damping ratios of six bending modes associated with the blade are estimated using real-time velocity measurements from one single point of the blade. A comparison with the usual impact hammer modal testing is performed to evaluate and establish the proposed approach’s main contributions. The developed modal parameter identification algorithms are implemented to run into a standard personal computer (PC) where the data acquisition system’s measurements are conditioned and processed. The results show the performance and the fast parametric estimation of the proposed algebraic identification approach.