bluuebunny/crossref_metadata_2025 huggingface dataset:
The Metabolomics Changes in Luria–Bertani Broth Medium under Different Sterilization Methods and Their Effects on Bacillus Growth
Authors: Wang Haifeng , Guo Juan , Chen Xing , He Hongxuan
Published: August 2023

Luria–Bertani broth (LB) culture medium is a commonly used bacterial culture medium in the laboratory. The nutrient composition, concentration, and culture conditions of LB medium can influence the growth of microbial strains. The purpose of this article is to demonstrate the impact of LB liquid culture medium on microbial growth under different sterilization conditions. In this study, LB medium with four different treatments was used, as follows: A, LB medium without treatments; B, LB medium with filtration; C, LB medium with autoclaving; and D, LB medium with autoclaving and cultured for 12 h. Subsequently, the protein levels and antioxidant capacity of the medium with different treatments were measured, and the effects of the different LB medium treatments on the growth of microorganisms and metabolites were determined via 16s rRNA gene sequencing and metabolomics analysis, respectively. Firmicutes and Lactobacillus were the dominant microorganisms, which were enriched in fermentation and chemoheterotrophy. The protein levels and antioxidant capacity of the LB medium with different treatments were different, and with the increasing concentration of medium, the protein levels were gradually increased, while the antioxidant capacity was decreased firstly and then increased. The growth trend of Bacillus subtilis, Bacillus paralicheniformis, Micrococcus luteus, and Alternaria alternata in the medium with different treatments was similar. Additionally, 220 and 114 differential metabolites were found between B and C medium, and between C and D medium, which were significantly enriched in the “Hedgehog signaling pathway”, “biosynthesis of plant secondary metabolites”, “ABC transporters”, “arginine and proline metabolism”, and “linoleic acid metabolism”. LB medium may be a good energy source for Lactobacillus growth with unsterilized medium, and LB medium filtered with a 0.22 μm filter membrane may be used for bacterial culture better than culture medium after high-pressure sterilization. LB medium still has the ability for antioxidation and to keep bacteria growth whether or not autoclaved, indicating that there are some substances that can resist a high temperature and pressure and still maintain their functions.
Editorial for the Special Issue on Recent Advances in Reactive Ion Etching and Applications of High-Aspect-Ratio Microfabrication
Authors: Romano Lucia , Jefimovs Konstantins
Published: August 2023

Reactive ion etching (RIE) is the dominating technology for micromachining semiconductors with a high aspect ratio (HAR) [...]
A Low-Cost Multibeam Switching Antenna Using Reconfigurable Hybrid Metasurface for Beamforming Applications
Authors: Sheng Lili , Luo Yumei , Ning Gangxin , Meng Liang , Cao Weiping
Published: August 2023

In this paper, we proposed a multibeam switching antenna based on a low-cost reconfigurable hybrid metasurface applied for beamforming systems. The antenna consists of two parts: a microstrip feed antenna and a transmission hybrid metasurface. The latter is composed of three types of units with different amplitude and phase responses to electromagnetic waves so as to control the beams of the feed antenna. Sixteen PIN diodes are arranged in the metasurface with a simple bias network. When two different direct-current voltages are applied to the PIN diodes, the antenna can dynamically switch between two beams and four beams. For demonstration, the proposed antenna is fabricated, and the measured results show that the antenna operates at 9.07–9.42 GHz (−10 dB bandwidth) with a total size of 1.80λ0 × 1.52λ0 × 0.22λ0 (λ0 corresponds to the wavelength of 9.28 GHz in free space). With the merits of a compact structure, low cost and good radiation performance, the proposed design is suitable for beamforming applications.
Simultaneous Hydrostatic and Compressive Loading System for Mimicking the Mechanical Environment of Living Cartilage Tissue
Authors: Chang Minki , Takahashi Yosuke , Miyahira Kyosuke , Omuro Yuma , Montagne Kevin , Yamada Ryusei , Gondo Junki , Kambe Yu , Yasuno Masashi , Masumoto Noriyasu , Ushida Takashi , Furukawa Katsuko S.
Published: August 2023

In vivo, articular cartilage tissue is surrounded by a cartilage membrane, and hydrostatic pressure (HP) and compressive strain increase simultaneously with the compressive stress. However, it has been impossible to investigate the effects of simultaneous loading in vitro. In this study, a bioreactor capable of applying compressive stress under HP was developed to reproduce ex vivo the same physical loading environment found in cartilage. First, a HP stimulation unit was constructed to apply a cyclic HP pressure-resistant chamber by controlling a pump and valve. A compression-loading mechanism that can apply compressive stress using an electromagnetic force was implemented in the chamber. The synchronization between the compression and HP units was evaluated, and the stimulation parameters were quantitatively evaluated. Physiological HP and compressive strain were applied to the chondrocytes encapsulated in alginate and gelatin gels after applying high HP at 25 MPa, which induced damage to the chondrocytes. It was found that compressive stimulation increased the expression of genes related to osteoarthritis. Furthermore, the simultaneous application of compressive strain and HP, which is similar to the physiological environment in cartilage, had an inhibitory effect on the expression of genes related to osteoarthritis. HP alone also suppressed the expression of osteoarthritis-related genes. Therefore, the simultaneous hydrostatic and compressive stress-loading device developed to simulate the mechanical environment in vivo may be an important tool for elucidating the mechanisms of disease onset and homeostasis in cartilage.
Isolation and Characterization of Lactic Acid Bacteria from Cocoa Mucilage and Meat: Exploring Their Potential as Biopreservatives for Beef
Authors: Morante-Carriel Laura , Abasolo Fernando , Bastidas-Caldes Carlos , Paz Erwin A. , Huaquipán Rodrigo , Díaz Rommy , Valdes Marco , Cancino David , Sepúlveda Néstor , Quiñones John
Published: August 2023

The aim of this study was to characterize lactic acid bacteria (LAB) isolated from cocoa mucilage and beef and evaluate their inhibitory effect in vitro against pathogenic bacteria, as well as determine their effect on beef quality. For the antagonist assay, 11 strains of LAB were selected and tested against pathogenic strains of Escherichia coli and Salmonella sp. The pathogenic bacteria were cultured in a medium, and a previously reactivated LAB bacterial pellet was added. After incubation, halos were observed around the bacterial colonies of the pathogenic strains, indicating inhibition by the LAB. It was identified that the LAB strains used belonged to the genus Lactobacillus, and the CCN-5 strain showed high percentages of inhibition against Salmonella sp. (58.33%) and E. coli (59%). The effectiveness of LAB application methods (immersion, injection, and spraying) did not present statistical differences. Furthermore, no significant changes in the physicochemical characteristics of beef were observed after the application of LAB. The results obtained demonstrate the potential of cocoa mucilage, as a biological control agent through LAB application, for beef biopreservation due to its ability to inhibit the growth of pathogenic bacteria.
Virtual Urban Field Studies: Evaluating Urban Interaction Design Using Context-Based Interface Prototypes
Authors: Dongas Robert , Grace Kazjon , Gillespie Samuel , Hoggenmueller Marius , Tomitsch Martin , Worrall Stewart
Published: August 2023

In this study, we propose the use of virtual urban field studies (VUFS) through context-based interface prototypes for evaluating the interaction design of auditory interfaces. Virtual field tests use mixed-reality technologies to combine the fidelity of real-world testing with the affordability and speed of testing in the lab. In this paper, we apply this concept to rapidly test sound designs for autonomous vehicle (AV)–pedestrian interaction with a high degree of realism and fidelity. We also propose the use of psychometrically validated measures of presence in validating the verisimilitude of VUFS. Using mixed qualitative and quantitative methods, we analysed users’ perceptions of presence in our VUFS prototype and the relationship to our prototype’s effectiveness. We also examined the use of higher-order ambisonic spatialised audio and its impact on presence. Our results provide insights into how VUFS can be designed to facilitate presence as well as design guidelines for how this can be leveraged.
Sensing Approaches Exploiting Molecularly Imprinted Nanoparticles and Lossy Mode Resonance in Polymer Optical Fibers
Authors: Arcadio Francesco , Noël Laurent , Del Prete Domenico , Seggio Mimimorena , Zeni Luigi , Bossi Alessandra , Soppera Olivier , Cennamo Nunzio
Published: August 2023

In this work, two different lossy mode resonance (LMR) platforms based on plastic optical fibers (POFs) are developed and tested in a biochemical sensing scenario. The LMR platforms are based on the combination of two metal oxides (MOs), i.e., zirconium oxide (ZrO2) and titanium oxide (TiO2), and deposited on the exposed core of D-shaped POF chips. More specifically, two experimental sensor configurations were obtained by swapping the mutual position of the Mos films over to the core of the D-shaped POF probe. The POF–LMR sensors were first characterized as refractometers, proving the bulk sensitivities. Then, both the POF–LMR platforms were functionalized using molecularly imprinted nanoparticles (nanoMIPs) specific for human transferrin (HTR) in order to carry out binding tests. The achieved results report a bulk sensitivity equal to about 148 nm/RIU in the best sensor configuration, namely the POF-TiO2-ZrO2. In contrast, both optical configurations combined with nanoMIPs showed an ultra-low detection limit (fM), demonstrating excellent efficiency of the used receptor (nanoMIPs) and paving the way to disposable POF–LMR biochemical sensors that are easy-to-use, low-cost, and highly sensitive.
The Influence of Reduced Graphene Oxide on the Texture and Chemistry of N,S-Doped Porous Carbon. Implications for Electrocatalytic and Energy Storage Applications
Authors: Samaniego Andrade Samantha K. , Lakshmi Shiva Shankar , Bakos István , Klébert Szilvia , Kun Robert , Mohai Miklós , Nagy Balázs , László Krisztina
Published: August 2023

In this work, we study the influence of reduced graphene oxide (rGO) on the morphology and chemistry of highly porous N,S-doped carbon cryogels. Simultaneously, we propose an easily upscalable route to prepare such carbons by adding graphene oxide (GO) in as-received suspended form to the aqueous solution of the ι-carrageenan and urea precursors. First, 1.25–5 wt% GO was incorporated into the dual-doped polymer matrix. The CO2, CO, and H2O emitted during the thermal treatments resulted in the multifaceted modification of the textural and chemical properties of the porous carbon. This facilitated the formation of micropores through self-activation and resulted in a substantial increase in the apparent surface area (up to 1780 m2/g) and pore volume (up to 1.72 cm3/g). However, adding 5 wt% GO led to overactivation. The incorporated rGO has an ordering effect on the carbon matrix. The evolving oxidative species influence the surface chemistry in a complex way, but sufficient N and S atoms (ca. 4 and >1 at%, respectively) were preserved in addition to the large number of developing defects. Despite the complexity of the textural and chemical changes, rGO increased the electrical conductivity monotonically. In alkaline oxygen reduction reaction (ORR) tests, the sample with 1.25 wt% GO exhibited a 4e− mechanism and reasonable stability, but a higher rGO content gradually compromised the performance of the electrodes. The sample containing 5 wt% GO was the most sensitive under oxidative conditions, but after stabilization it exhibited the highest gravimetric capacitance. In Li-ion battery tests, the coulombic efficiency of all the samples was consistently above 98%, indicating the high potential of these carbons for efficient Li-ion insertion and reinsertion during the charge–discharge process, thereby providing a promising alternative for graphite-based anodes. The cell from the 1.25 wt% GO sample showed an initial discharge capacity of 313 mAh/g, 95.1% capacity retention, and 99.3% coulombic efficiency after 50 charge–discharge cycles.
Hybrid Mesoporous Carbon/Copper Ferrite Electrode for Asymmetric Supercapacitors
Authors: Huynh Khang , Maddipudi Bharathkiran , Shende Rajesh
Published: August 2023

Asymmetric supercapacitors (ASCs) with two dissimilar electrodes are known to exhibit relatively moderate energy and power densities. If electrodes derived from earth-abundant materials or renewable resources such as lignocellulosic biomass (LCB) are used for fabrication, energy storage systems are expected to become less expensive and more sustainable. Hybrid electrode materials have advantages such as higher surface area, better chemical stability, and superior energy density. This study reports on the synthesis of a novel hybrid electrode material containing porous carbon (POC) and copper ferrite, which is designated as POC@Cu-ferrite, and its electrochemical performance in ASC configuration. Corn stover derived hydrochar is utilized for the sol–gel synthesis of POC@Cu-ferrite hybrid material using earth-abundant Cu and Fe-based precursors. This material is characterized using X-ray diffraction (XRD), Raman spectroscopy, Brunauer–Emmett–Teller (BET) surface area analyzer, and scanning and transmission electron microscopy (SEM/TEM). As-synthesized Cu-ferrite is found to contain 89.2% CuFe2O4 and 10.8% Fe2O3, whereas other phases such as Fe3O4, CuFeO2, and CuO are observed for the POC@Cu-ferrite. BET-specific surface area (SSA) and pore volume of POC@Cu-ferrite are observed as 1068 m2/g and 0.72 cm3/g, respectively. POC@Cu-ferrite hybrid electrode is used with POC opposite electrode to fabricate ASC, which is tested using Gamry G-300 potentiostat/galvanostat/ZRA to obtain cyclic voltammetry (CV) profiles and galvanostatic charge–discharge (GCD) plots. ASC is also prepared using Cu-ferrite and POC materials and its specific capacitance and stability are compared with ASCs prepared with POC@Cu-ferrite and POC or graphene nanoplatelets (GNPs) electrodes. POC@Cu-ferrite hybrid electrode is found to be superior with a 2-fold higher capacitance and significant electrochemical stability over 100 GCD cycles as compared to the Cu-ferrite electrode.
Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
Authors: Malanina Anastasiya , Kuzin Yurii , Khadieva Alena , Shibaeva Kseniya , Padnya Pavel , Stoikov Ivan , Evtugyn Gennady
Published: August 2023

A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose, a monomeric phenothiazine derivative has been deposited on the glassy carbon electrode from the 0.4 M H2SO4-acetone mixture (1:1 v/v) by multiple potential cycling. The DNA aliquot was either on the electrode modified with electropolymerized film or added to the reaction medium prior to electropolymerization. The DNA entrapment and its influence on the redox behavior of the underlying layer were studied by scanning electron microscopy and electrochemical impedance spectroscopy. The DNA–doxorubicin interactions affected the charge distribution in the surface layer and, hence, altered the redox equilibrium of the polyphenothiazine coating. The voltametric signal was successfully applied for the determination of doxorubicin in the concentration range from 10 pM to 0.2 mM (limit of detection 5 pM). The DNA sensor was tested on spiked artificial plasma samples and two commercial medications (recovery of 90–95%). After further testing on real clinical samples, the electrochemical DNA sensor developed can find application in monitoring drug release and screening new antitumor drugs able to intercalate DNA.
Plasma One-Carbon Metabolism-Related Micronutrients and the Risk of Breast Cancer: Involvement of DNA Methylation
Authors: Liu Fubin , Zhou Huijun , Peng Yu , Qiao Yating , Wang Peng , Si Changyu , Wang Xixuan , Gong Jianxiao , Chen Kexin , Song Fangfang
Published: August 2023

Findings of epidemiologic studies focusing on the association between one-carbon metabolism-related micronutrients and breast cancer risk, along with the involvement of DNA methylation, have been inconsistent and incomprehensive. We conducted a case–control study in China including 107 paired participants and comprehensively detected 12 plasma one-carbon metabolism-related micronutrients. Genomic DNA methylation was measured using an 850 K chip and differential methylation probes (DMPs) were identified. Multivariate logistic regression was performed to estimate the associations between plasma micronutrients and the odds of breast cancer. The mediation of selected DMPs in micronutrient breast cancer associations was examined using mediation analyses. An inverse association of plasma folate, methionine cycling-related micronutrients (methionine, S-adenosylmethionine, and S-adenosylhomocysteine), and all micronutrients in the choline metabolism and enzymatic factor groups, and a positive association of methionine cycling-related cysteine with breast cancer risk were observed. Nine micronutrients (methionine, cysteine, SAM, folate, choline, betaine, P5P, vitamins B2, and B12) were related to global or probe-specific methylation levels (p < 0.05). The selected DMPs mediated the micronutrient breast cancer associations with an average mediation proportion of 36.43%. This study depicted comprehensive associations between circulating one-carbon metabolism-related micronutrients and breast cancer risk mediated by DNA methylation.
Controlling Chronic Diseases and Acute Infections with Vitamin D Sufficiency
Authors: Wimalawansa Sunil J.
Published: August 2023

Apart from developmental disabilities, the prevalence of chronic diseases increases with age especially in those with co-morbidities: vitamin D deficiency plays a major role in it. Whether vitamin D deficiency initiates and/or aggravates chronic diseases or vice versa is unclear. It adversely affects all body systems but can be eliminated using proper doses of vitamin D supplementation and/or safe daily sun exposure. Maintaining the population serum 25(OH)D concentration above 40 ng/mL (i.e., sufficiency) ensures a sound immune system, minimizing symptomatic diseases and reducing infections and the prevalence of chronic diseases. This is the most cost-effective way to keep a population healthy and reduce healthcare costs. Vitamin D facilitates physiological functions, overcoming pathologies such as chronic inflammation and oxidative stress and maintaining broader immune functions. These are vital to overcoming chronic diseases and infections. Therefore, in addition to following essential public health and nutritional guidance, maintaining vitamin D sufficiency should be an integral part of better health, preventing acute and chronic diseases and minimize their complications. Those with severe vitamin D deficiency have the highest burdens of co-morbidities and are more vulnerable to developing complications and untimely deaths. Vitamin D adequacy improves innate and adaptive immune systems. It controls excessive inflammation and oxidative stress, generates antimicrobial peptides, and neutralizes antibodies via immune cells. Consequently, vitamin D sufficiency reduces infections and associated complications and deaths. Maintaining vitamin D sufficiency reduces chronic disease burden, illnesses, hospitalizations, and all-cause mortality. Vulnerable communities, such as ethnic minorities living in temperate countries, older people, those with co-morbidities, routine night workers, and institutionalized persons, have the highest prevalence of vitamin D deficiency—they would significantly benefit from vitamin D and targeted micronutrient supplementation. At least now, health departments, authorities, and health insurance companies should start assessing, prioritizing, and encouraging this economical, non-prescription, safe micronutrient to prevent and treat acute and chronic diseases. This approach will significantly reduce morbidity, mortality, and healthcare costs and ensure healthy aging.
Effects of an Eating Pattern Including Colorful Fruits and Vegetables on Management of Gestational Diabetes: A Randomized Controlled Trial
Authors: Jaworsky Kataryna , DeVillez Pamela , Alexander James M. , Basu Arpita
Published: August 2023

Gestational diabetes mellitus (GDM), defined as abnormal glucose tolerance that presents during the second and third trimesters of pregnancy, is a growing issue in the United States and worldwide. If left untreated or poorly controlled, GDM can result in numerous consequences for both the mother and the fetus; thus, it is imperative that different avenues of management for GDM be explored. There is a paucity of studies that examine how lifestyle changes, including dietary and physical activity, affect management of GDM. We examined how counseling on lifestyle changes can affect cardiometabolic risks in women with GDM. We conducted a 12-week randomized controlled trial based on behavioral counseling in which women with GDM (N = 38) were randomized into either a nutrition education (control) (N = 18) group or nutrition intervention (N = 20) group. The nutrition education group were given dietary counseling regarding healthy dietary choices based on USDA guidelines, while the nutrition intervention group were instructed to consume a total of one cup of whole berries and one cup of leafy vegetables daily along with performing postprandial exercise (walking). Blood samples, anthropometric measures, and dietary and physical activity data, recorded in daily food and activity logs, were collected at baseline and at the end of the study and compared between the two groups. Dietary counseling on supplementation with whole berries and leafy vegetables resulted in increased fiber intake, increased antioxidant intake and total serum antioxidant capacity, improved random blood glucose, decreased serum IL-6, and improved HDL cholesterol versus the control group (all p < 0.05). These results highlight that whole berry and leafy vegetable supplementation-based dietary counseling can improve the metabolic pathways involved in gestational diabetes pathogenesis and prognosis. These functional foods must be recommended in the management of pregnancies affected by GDM.
Effects of Oral Alpha-Lipoic Acid Treatment on Diabetic Polyneuropathy: A Meta-Analysis and Systematic Review
Authors: Hsieh Ruey-Yu , Huang I-Chen , Chen Chiehfeng , Sung Jia-Ying
Published: August 2023

Alpha-lipoic acid (ALA) was found to improve the symptoms in patients with diabetic sensorimotor peripheral neuropathy (DSPN) by reducing oxidative stress and ameliorating microcirculation. Our meta-analysis is aimed at evaluating the effects of oral-administered ALA versus a placebo in patients with DSPN and determining the optimal dosage for this treatment. We systematically reviewed randomized controlled trials (RCTs) in the PubMed, Embase, and Cochrane databases to determine the efficacy of oral ALA for patients with DSPN. The primary outcome was total symptoms’ score (TSS), and secondary outcomes were the neurological disability score (NDS), neuropathy impaired score (NIS), NIS-lower limb (NIS-LL), vibration perception threshold (VPT), nerve conduction study (NCS) results, and global satisfaction. A subgroup analysis of the ALA dosage (600, 1200, and 1800 mg/day) was also conducted. Ten RCTs (1242 patients) were included. ALA treatment produced favorable results for TSS (a dose-related trend was observed), NDS, and the global satisfaction score. For VAS, VPT, NIS-LL, and NCS results, ALA did not produce favorable results. ALA treatment had favorable effects on DSPN by reducing sensory symptoms, and it resulted in a dose-dependent response relative to the placebo for TSS and the global satisfaction score. The use of ALA to prevent neurological symptoms should be further researched.
Additive Effects of Cyclic Peptide [R4W4] When Added Alongside Azithromycin and Rifampicin against Mycobacterium avium Infection
Authors: Kelley Melissa , Sasaninia Kayvan , Abnousian Arbi , Badaoui Ali , Owens James , Beever Abrianna , Kachour Nala , Tiwari Rakesh Kumar , Venketaraman Vishwanath
Published: August 2023

Mycobacterium avium (M. avium), a type of nontuberculous mycobacteria (NTM), poses a risk for pulmonary infections and disseminated infections in immunocompromised individuals. Conventional treatment consists of a 12-month regimen of the first-line antibiotics rifampicin and azithromycin. However, the treatment duration and low antibiotic tolerability present challenges in the treatment of M. avium infection. Furthermore, the emergence of multidrug-resistant mycobacterium strains prompts a need for novel treatments against M. avium infection. This study aims to test the efficacy of a novel antimicrobial peptide, cyclic [R4W4], alongside the first-line antibiotics azithromycin and rifampicin in reducing M. avium survival. Colony-forming unit (CFU) counts were assessed after treating M. avium cultures with varying concentrations of cyclic [R4W4] alone or in conjunction with azithromycin or rifampicin 3 h and 4 days post-treatment. M. avium growth was significantly reduced 4 days after cyclic [R4W4] single treatment. Additionally, cyclic [R4W4]–azithromycin and cyclic [R4W4]–rifampicin combination treatments at specific concentrations significantly reduced M. avium survival 3 h and 4 days post-treatment compared with single antibiotic treatment alone. These findings demonstrate cyclic [R4W4] as a potent treatment method against M. avium and provide insight into novel therapeutic approaches against mycobacterium infections.
Bovine Papillomavirus Type 1 Infection in an Equine Congenital Papilloma
Authors: Maggi Raffaella , De Paolis Livia , De Santis Daria , Vellone Valerio Gaetano , De Ciucis Chiara Grazia , Fruscione Floriana , Mazzocco Katia , Ghelardi Alessandro , Marruchella Giuseppe , Razzuoli Elisabetta
Published: August 2023

Papillomas are benign epithelial lesions protruding on the epithelial surfaces as finger-like or warty projections. These lesions are often caused by papillomavirus (PV) infection. Congenital papillomas have been reported in foals. However, to date, no evidence of PV infection has been provided. In the present paper, we describe the main clinical–pathological features of a congenital papilloma observed in a foal. In addition, biomolecular tests demonstrated BPV1 infection in the case under study. Such data stimulate further investigations, even on archived samples, aiming to clarifying the etiology of equine congenital papilloma and the clinical relevance, if any, of BPV1 vertical transmission in horses.
Neuropharmacological Effects of the Dichloromethane Extract from the Stems of Argemone ochroleuca Sweet (Papaveraceae) and Its Active Compound Dihydrosanguinarine
Authors: Yáñez-Barrientos Eunice , Barragan-Galvez Juan Carlos , Hidalgo-Figueroa Sergio , Reyes-Luna Alfonso , Gonzalez-Rivera Maria L. , Cruz Cruz David , Isiordia-Espinoza Mario Alberto , Deveze-Álvarez Martha Alicia , Villegas Gómez Clarisa , Alonso-Castro Angel Josabad
Published: August 2023

Argemone ochroleuca Sweet (Papaveraceae) is used in folk medicine as a sedative and hypnotic agent. This study aimed to evaluate the anxiolytic-like, sedative, antidepressant-like, and anticonvulsant activities of a dichloromethane extract of A. ochroleuca stems (AOE), chemically standardized using gas chromatography–mass spectrometry (GC–MS), and its active compound dihydrosanguinarine (DHS). The anxiolytic-like, sedative, antidepressant-like, and anticonvulsant activities of the AOE (0.1–50 mg/kg p.o.) and DHS (0.1–10 mg/kg p.o.) were evaluated using murine models. A possible mechanism for the neurological actions induced by the AOE or DHS was assessed using inhibitors of neurotransmission pathways and molecular docking. Effective dose 50 (ED50) values were calculated by a linear regression analysis. The AOE showed anxiolytic-like activity in the cylinder exploratory test (ED50 = 33 mg/kg), and antidepressant-like effects in the forced swimming test (ED50 = 3 mg/kg) and the tail suspension test (ED50 = 23 mg/kg), whereas DHS showed anxiolytic-like activity (ED50 = 2 mg/kg) in the hole board test. The AOE (1–50 mg/kg) showed no locomotive affectations or sedation in mice. A docking study revealed the affinity of DHS for α2-adrenoreceptors and GABAA receptors. The anxiolytic-like and anticonvulsant effects of the AOE are due to GABAergic participation, whereas the antidepressant-like effects of the AOE are due to the noradrenergic system. The noradrenergic and GABAergic systems are involved in the anxiolytic-like actions of DHS.
Taste-Masked Flucloxacillin Powder Part 2: Formulation Optimisation Using the Mixture Design Approach and Storage Stability
Authors: Yoo Okhee , Salman Sam , von Ungern-Sternberg Britta S. , Lim Lee Yong
Published: August 2023

Flucloxacillin is prescribed to treat skin infections but its highly bitter taste is poorly tolerated in children. This work describes the application of the D-optimal mixture experimental design to identify the optimal component ratio of flucloxacillin, Eudragit EPO and palmitic acid to prepare flucloxacillin taste-masked microparticles that would be stable to storage and would inhibit flucloxacillin release in the oral cavity while facilitating the total release of the flucloxacillin load in the lower gastrointestinal tract (GIT). The model predicted ratio was found to be very close to the stoichiometric equimolar component ratio, which supported our hypothesis that the ionic interactions among flucloxacillin, Eudragit EPO and palmitic acid underscore the polyelectrolyte complex formation in the flucloxacillin taste-masked microparticles. The excipient–drug interactions showed protective effects on the microparticle storage stability and minimised flucloxacillin release at 2 min in dissolution medium. These interactions had less influence on flucloxacillin release in the dissolution medium at 60 min. Storage temperature and relative humidity significantly affected the chemical stability of the microparticles. At the preferred storage conditions of ambient temperature under reduced RH of 23%, over 90% of the baseline drug load was retained in the microparticles at 12 months of storage.
Denoising of Laser Self-Mixing Interference by Improved Wavelet Threshold for High Performance of Displacement Reconstruction
Authors: Liu Hui , You Yaqiang , Li Sijia , He Dan , Sun Jian , Wang Jingwei , Hou Dong
Published: August 2023

This article proposes an improved wavelet threshold denoising for laser self-mixing interference signals. The improved wavelet threshold function exhibits smoothness and continuity near the threshold. By replacing hard or soft wavelet threshold with the improved wavelet threshold, it can eliminate the generation of fake self-mixing interference peaks due to local oscillation induced by hard wavelet threshold, as well as the loss of self-mixing interference peaks due to over-smoothness induced by the soft wavelet threshold. Compared with hard and soft wavelet threshold denoising, theoretical simulations and experimental results demonstrate that the displacement of vibrations are well reconstructed based on the improved wavelet threshold denoising.
Advantages of Using Hard X-ray Photons for Ultrafast Diffraction Measurements
Authors: Lipp Vladimir , Inoue Ichiro , Ziaja Beata
Published: August 2023

We present a comparative theoretical study of silicon crystals irradiated with X-ray free-electron laser pulses, using hard X-ray photons of various energies. Simulations are performed with our in-house hybrid code XTANT based on Monte Carlo, Tight Binding and Molecular Dynamics simulation techniques. By comparing the strength of the coherently scattered signal and the corresponding electronic radiation damage for three X-ray photon energies available at the SACLA free-electron laser facility, we conclude that it would be beneficial to use higher photon energies for “diffraction-before-destruction” experiments.
Hyperspectral Channel-Modulated Static Birefringent Fourier Transform Imaging Spectropolarimeter with Zoomable Spectral Resolution
Authors: Zhang Xiangzhe , Zhu Jingping , Huang Liqing , Zhang Yu , Wang Huimin , Li Haoxiang , Guo Fengqi , Deng Jinxin
Published: August 2023

A novel channel-modulated static birefringent Fourier transform imaging spectropolarimeter (CSBFTIS) is introduced, which is based on a double Wollaston prism (DWP). With an adjustable air gap (AG), the spectral resolution can be adjusted by changing the AG. The CSBFTIS combines the channel-modulated imaging spectropolarimeter and the slit-free static birefringent Fourier transform imaging spectrometer technology with adjustable spectral resolution. The device is compact and robust, with a wide spectral range and a large luminous flux. Compared with various previous spectropolarimeters, it can greatly reduce the size of the spectral image data to adapt to different application requirements. A prototype is built, and simulation and experiments are carried out, and the results prove the effectiveness of the method.
Changes in Profiles of Classes and of Individual Polyphenols in Leaves of Spiraea chamaedryfolia and Spiraea media along an Altitudinal Gradient
Authors: Boyarskikh Irina G. , Artemov Igor A. , Kuznetsov Alexander A. , Kostikova Vera A.
Published: August 2023

Plants in high-altitude habitats are exposed to severe environmental stressors, including extreme temperatures and irradiation, which can have wide-ranging effects on changes of secondary-metabolite profiles in higher plants. Altitude-related variation of levels of polyphenols in organs of medicinal and food plant species has not yet been investigated sufficiently. This study was focused on variation in quantitative profiles of classes and of individual biologically active phenolic compounds in leaf extracts of resource species Spiraea chamaedryfolia and Spiraea media from the family Rosaceae in coenopopulations of the Altai Mountains, along an altitudinal gradient. High-performance liquid chromatography revealed 22 polyphenolic compounds in the extracts of S. media leaves, with the main polyphenolic compounds being flavonols. Sixteen compounds were found in S. chamaedryfolia leaf extracts, and the major ones were flavonols and a flavanone. Opposite responses to changes in the altitude-associated growth conditions were documented for levels of some individual polyphenolic compounds. With an increase in altitude, concentrations of chlorogenic acid and of flavanone in the extracts of S. chamaedryfolia leaves significantly increased, while concentrations of cinnamic acid, astragalin, and kaempferol diminished. A statistically significant positive correlation between the altitude of plant habitats and total levels of polyphenols and phenolcarboxylic acids was detected. In leaf extracts from S. media, an altitude increase was significantly positively correlated with astragalin, avicularin, and cinnamic acid levels and negatively correlated with hyperoside concentration.
Thermal and Mechanical Properties of Reprocessed Polylactide/Titanium Dioxide Nanocomposites for Material Extrusion Additive Manufacturing
Authors: Bergaliyeva Saltanat , Sales David L. , Jiménez Cabello José María , Burgos Pintos Pedro , Fernández Delgado Natalia , Marzo Gago Patricia , Zammit Ann , Molina Sergio I.
Published: August 2023

Polylactic acid (PLA) is a biodegradable polymer that can replace petroleum-based polymers and is widely used in material extrusion additive manufacturing (AM). The reprocessing of PLA leads to a downcycling of its properties, so strategies are being sought to counteract this effect, such as blending with virgin material or creating nanocomposites. Thus, two sets of nanocomposites based respectively on virgin PLA and a blend of PLA and reprocessed PLA (rPLA) with the addition of 0, 3, and 7 wt% of titanium dioxide nanoparticles (TiO2) were created via a double screw extruder system. All blends were used for material extrusion for 3D printing directly from pellets without difficulty. Scanning electron micrographs of fractured samples’ surfaces indicate that the nanoparticles gathered in agglomerations in some blends, which were well dispersed in the polymer matrix. The thermal stability and degree of crystallinity for every set of nanocomposites have a rising tendency with increasing nanoparticle concentration. The glass transition and melting temperatures of PLA/TiO2 and PLA/rPLA/TiO2 do not differ much. Tensile testing showed that although reprocessed material implies a detriment to the mechanical properties, in the specimens with 7% nano-TiO2, this effect is counteracted, reaching values like those of virgin PLA.
Influence of Ethylene Oxide and Gamma Irradiation Sterilization Processes on the Properties of Poly-L-Lactic-Acid (PLLA) Materials
Authors: Krug Natalie , Zarges Jan-Christoph , Heim Hans-Peter
Published: August 2023

In order to encourage the substitution of petrochemical polymers in medical technology with sustainable, bio-based materials, there is an urgent need for further investigations, especially data regarding their sterility performance. Within the scope of the investigations, selected material properties of poly-L-lactic-acid (PLLA), a specific type of poly(lactic-acid) (PLA), were analyzed before and after sterilization (using ethylene oxide or gamma irradiation) in order to investigate deviations in its chemical structure, wettability, optical, and mechanical properties. In particular, parameters such as molecular weight, complex viscosity, tensile strength, water contact angle, and color were discussed. Sterilization temperatures close to the glass transition of PLA, high humidity, and interactions with the ethylene oxide molecules have resulted in an increase in crystallinity, a decrease in elongation at break, and in some cases, a variation in wettability. As a consequence of exposure to high-energy radiation, the material’s toughness is reduced due to chain scission, which is manifested through a decrease in molecular weight, an increase in crystallinity, and a partial change in surface energy. For the selected PLLA-materials (Luminy® L130, NP HT 202, and NP HT 203), ethylene oxide sterilization resulted in a comparatively minor variation in the characteristics behavior, and was chosen as the preferred method.
On the Coherence of Mencius’ Concept of Li: An Analysis Based on Moral Reasons Internalism
Authors: Liu Shuwen , Xie Xiaodong
Published: August 2023

This paper addresses the problem of coherence, i.e., whether the internal and external dimensions of li (礼 rituals, rites, and the observance of them) are compatible. This problem stems mainly from Mencius’ seemingly conflicting statements. On the one hand, he emphasized the goodness of xing (性 human nature) in terms of the goodness of the heart-mind. On the other hand, he defended the act of following li regardless of one’s heart-mind. This paper argues that Mencius held coherent moral reasons internalism, asserting that moral reasons are embedded in people’s universal and potential instinct to do morally good things. Consequently, he distinguished morality from non-moral normativity and claimed that the former takes precedence over the latter. The concept of li is thus divided into moral li and non-moral li, with moral li taking precedence over non-moral li. Therefore, the act of “following li regardless of one’s heart-mind” refers to following non-moral li, as long as it does not conflict with moral li. Based on the fact that Mencius’ concept of li is a kind of coherent moral reasons internalism, this paper further responds to some challenges from moral reasons externalism. It does so by clarifying the meaning of “seeking the cause within oneself” (反求诸己 fan qiu zhu ji), showing that Mencius believed every individual could establish rational inner guidance and be motivated to lead a reflective and autonomous moral life.
Anti-Cult Movement and Religious Freedom for Religious Minorities in the Russian Arctic
Authors: Beliakova Nadezhda , Kliueva Vera
Published: August 2023

In this article, using the example of the Russian Arctic, we analyze how the anti-cult movement creates the conditions for the discrimination and suppression of religious minorities. The anti-cultist (anti-sectarian) fears and phobias of the Russian establishment are closely bound to the fears of missionary activity. The change in legislation regulating missionary activity deliberately limits the activities of those religious communities, which, at the suggestion of the anti-cultists, are labeled in Russian society as “sectarian” and/or “foreign”.
Blind Restoration of a Single Real Turbulence-Degraded Image Based on Self-Supervised Learning
Authors: Guo Yiming , Wu Xiaoqing , Qing Chun , Liu Liyong , Yang Qike , Hu Xiaodan , Qian Xianmei , Shao Shiyong
Published: August 2023

Turbulence-degraded image frames are distorted by both turbulent deformations and space–time varying blurs. Restoration of the atmospheric turbulence-degraded image is of great importance in the state of affairs, such as remoting sensing, surveillance, traffic control, and astronomy. While traditional supervised learning uses lots of simulated distorted images for training, it has poor generalization ability for real degraded images. To address this problem, a novel blind restoration network that only inputs a single turbulence-degraded image is presented, which is mainly used to reconstruct the real atmospheric turbulence distorted images. In addition, the proposed method does not require pre-training, and only needs to input a single real turbulent degradation image to output a high-quality result. Meanwhile, to improve the self-supervised restoration effect, Regularization by Denoising (RED) is introduced to the network, and the final output is obtained by averaging the prediction of multiple iterations in the trained model. Experiments are carried out with real-world turbulence-degraded data by implementing the proposed method and four reported methods, and we use four non-reference indicators for evaluation, among which Average Gradient, NIQE, and BRISQUE have achieved state-of-the-art effects compared with other methods. As a result, our method is effective in alleviating distortions and blur, restoring image details, and enhancing visual quality. Furthermore, the proposed approach has a certain degree of generalization, and has an excellent restoration effect for motion-blurred images.
Quantifying the Loss of Coral from a Bleaching Event Using Underwater Photogrammetry and AI-Assisted Image Segmentation
Authors: Kopecky Kai L. , Pavoni Gaia , Nocerino Erica , Brooks Andrew J. , Corsini Massimiliano , Menna Fabio , Gallagher Jordan P. , Capra Alessandro , Castagnetti Cristina , Rossi Paolo , Gruen Armin , Neyer Fabian , Muntoni Alessandro , Ponchio Federico , Cignoni Paolo , Troyer Matthias , Holbrook Sally J. , Schmitt Russell J.
Published: August 2023

Detecting the impacts of natural and anthropogenic disturbances that cause declines in organisms or changes in community composition has long been a focus of ecology. However, a tradeoff often exists between the spatial extent over which relevant data can be collected, and the resolution of those data. Recent advances in underwater photogrammetry, as well as computer vision and machine learning tools that employ artificial intelligence (AI), offer potential solutions with which to resolve this tradeoff. Here, we coupled a rigorous photogrammetric survey method with novel AI-assisted image segmentation software in order to quantify the impact of a coral bleaching event on a tropical reef, both at an ecologically meaningful spatial scale and with high spatial resolution. In addition to outlining our workflow, we highlight three key results: (1) dramatic changes in the three-dimensional surface areas of live and dead coral, as well as the ratio of live to dead colonies before and after bleaching; (2) a size-dependent pattern of mortality in bleached corals, where the largest corals were disproportionately affected, and (3) a significantly greater decline in the surface area of live coral, as revealed by our approximation of the 3D shape compared to the more standard planar area (2D) approach. The technique of photogrammetry allows us to turn 2D images into approximate 3D models in a flexible and efficient way. Increasing the resolution, accuracy, spatial extent, and efficiency with which we can quantify effects of disturbances will improve our ability to understand the ecological consequences that cascade from small to large scales, as well as allow more informed decisions to be made regarding the mitigation of undesired impacts.
Random Shuffling Data for Hyperspectral Image Classification with Siamese and Knowledge Distillation Network
Authors: Yang Zhen , Cao Ying , Zhou Xin , Liu Junya , Zhang Tao , Ji Jinsheng
Published: August 2023

Hyperspectral images (HSIs) are characterized by hundreds of spectral bands. The goal of HSI is to associate the pixel with a corresponding category label by analyzing subtle differences in the spectrum. Due to their excellent local context modeling capabilities, Convolutional Neural Network (CNN)-based methods are often adopted to complete the classification task. To verify whether the patch-data-based CNN methods depend on the homogeneity of patch data during the training process in HSI classification, we designed a random shuffling strategy to disrupt the data homogeneity of the patch data, which is randomly assigning the pixels from the original dataset to other positions to form a new dataset. Based on this random shuffling strategy, we propose a sub-branch to extract features on the reconstructed dataset and fuse the loss rates (RFL). The loss rate calculated by RFL in the new patch data is cross combined with the loss value calculated by another sub-branch in the original patch data. Moreover, we construct a new hyperspectral classification network based on the Siamese and Knowledge Distillation Network (SKDN) that can improve the classification accuracy on randomly shuffled data. In addition, RFL is introduced into the original model for hyperspectral classification tasks in the original dataset. The experimental results show that the improved model is also better than the original model, which indicates that RFL is effective and feasible. Experiments on four real-world datasets show that, as the proportion of randomly shuffling data increases, the latest patch-data-based CNN methods cannot extract more abundant local contextual information for HSI classification, while the proposed sub-branch RFL can alleviate this problem and improve the network’s recognition ability.