bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional Near-Infrared Fluorescence Imaging for Cardiac Surgery and Targeted Gene Therapy
Authors: Nakayama Akira , del Monte Federica , Hajjar Roger J. , Frangioni John V.
Published: March 2018

Cardiac revascularization is presently performed without realtime visual assessment of myocardial blood flow or perfusion. Moreover, gene therapy of the heart cannot, at present, be directed to specific territories at risk for myocardial infarction. We have developed a surgical imaging system that exploits the low autofluorescence, deep tissue penetration, low tissue scatter, and invisibility of near-infrared (NIR) fluorescent light. By completely isolating visible and NIR light paths, one is able to visualize, simultaneously, the anatomy and/or function of the heart, or any desired tissue. In rat model systems, we demonstrate that the heptamethine indocyanine-type NIR fluorophores IR-786 and the carboxylic acid form of IRDye78 can be injected intravenously in the living animal to provide real-time visual assessment of myocardial blood flow or perfusion intraoperatively. This imaging system may prove useful for the refinement of revascularization techniques, and for the administration of cardiac gene therapy.
Molecular Imaging of Gliomas
Authors: Jacobs A. H. , Dittmar C. , Winkeler A. , Garlip G. , Heiss W. D.
Published: March 2018

Gliomas are the most common types of brain tumors. Although sophisticated regimens of conventional therapies are being carried out to treat patients with gliomas, the disease invariably leads to death over months or years. Before new and potentially more effective treatment strategies, such as gene- and cell-based therapies, can be effectively implemented in the clinical application, certain prerequisites have to be established. First of all, the exact localization, extent, and metabolic activity of the glioma must be determined to identify the biologically active target tissue for a biological treatment regimen; this is usually performed by imaging the expression of up-regulated endogenous genes coding for glucose or amino acid transporters and cellular hexokinase and thymidine kinase genes, respectively. Second, neuronal function and functional changes within the surrounding brain tissue have to be assessed in order to save this tissue from therapy-induced damage. Third, pathognomonic genetic changes leading to disease have to be explored on the molecular level to serve as specific targets for patient-tailored therapies. Last, a concerted noninvasive analysis of both endogenous and exogenous gene expression in animal models as well as the clinical setting is desirable to effectively translate new treatment strategies from experimental into clinical application. All of these issues can be addressed by multimodal radionuclide and magnetic resonance imaging techniques and fall into the exciting and fast growing field of molecular and functional imaging. Noninvasive imaging of endogenous gene expression by means of positron emission tomography (PET) may reveal insight into the molecular basis of pathogenesis and metabolic activity of the glioma and the extent of treatment response. When exogenous genes are introduced to serve for a therapeutic function, PET imaging may reveal the assessment of the “location,” “magnitude,” and “duration” of therapeutic gene expression and its relation to the therapeutic effect. Detailed reviews on molecular imaging have been published from the perspective of radionuclide imaging (Gambhir et al., 2000; Blasberg and Tjuvajev, 2002) as well as magnetic resonance and optical imaging (Weissleder, 2002). The present review focuses on molecular imaging of gliomas with special reference on the status and perspectives of imaging of endogenous and exogenously introduced gene expression in order to develop improved diagnostics and more effective treatment strategies of gliomas and, in that, to eventually improve the grim prognosis of this devastating disease.
Diffusion MRI: A New Strategy for Assessment of Cancer Therapeutic Efficacy
Authors: Chenevert Thomas L. , Meyer Charles R. , Moffat Bradford A. , Rehemtulla Alnawaz , Mukherji Suresh K. , Gebarski Stephen S. , Quint Douglas J. , Robertson Patricia L. , Lawrence Theodore S. , Junck Larry , Taylor Jeremy M. G. , Johnson Timothy D. , Dong Qian , Muraszko Karin M. , Brunberg James A. , Ross Brian D.
Published: March 2018

The use of anatomical imaging in clinical oncology practice traditionally relies on comparison of patient scans acquired before and following completion of therapeutic intervention. Therapeutic success is typically determined from inspection of gross anatomical images to assess changes in tumor size. Imaging could provide significant additional insight into therapeutic impact if a specific parameter or combination of parameters could be identified which reflect tissue changes at the cellular or physiologic level. This would provide an early indicator of treatment response/outcome in an individual patient before completion of therapy. Moreover, response of a tumor to therapeutic intervention may be heterogeneous. The use of imaging could assist in delineating therapeutic-induced spatial heterogeneity within a tumor mass by providing information related to specific regions that are resistant or responsive to treatment. Largely untapped potential resides in exploratory methods such as diffusion MRI, which is a non-volumetric intravoxel measure of tumor response based upon water molecular mobility. Alterations in water mobility reflect changes in tissue structure at the cellular level. While the clinical utility of diffusion MRI for oncologic practice is still under active investigation, this overview on the use of diffusion MRI for the evaluation of brain tumors will serve to introduce how this approach may be applied in the future for the management of patients with solid tumors.
Quantitation of Brown Adipose Tissue Perfusion in Transgenic Mice Using Near-Infrared Fluorescence Imaging
Authors: Nakayama Akira , Bianco Antonio C. , Zhang Chen-Yu , Lowell Bradford B. , Frangioni John V.
Published: March 2018

Brown adipose tissue (BAT; brown fat) is the principal site of adaptive thermogenesis in the human newborn and other small mammals. Of paramount importance for thermogenesis is vascular perfusion, which controls the flow of cool blood in, and warmed blood out, of BAT. We have developed an optical method for the quantitative imaging of BAT perfusion in the living, intact animal using the heptamethine indocyanine IR-786 and near-infrared (NIR) fluorescent light. We present a detailed analysis of the physical, chemical, and cellular properties of IR-786, its biodistribution and pharmacokinetics, and its uptake into BAT. Using transgenic animals with homozygous deletion of Type II iodothyronine deiodinase, or homozygous deletion of uncoupling proteins (UCPs) 1 and 2, we demonstrate that BAT perfusion can be measured noninvasively, accurately, and reproducibly. Using these techniques, we show that UCP 1/2 knockout animals, when compared to wild-type animals, have a higher baseline perfusion of BAT but a similar maximal response to β3-receptor agonist. These results suggest that compensation for UCP deletion is mediated, in part, by the control of BAT perfusion. Taken together, BAT perfusion can now be measured noninvasively using NIR fluorescent light, and pharmacological modulators of thermogenesis can be screened at relatively high throughput in living animals.
Choline Phospholipid Metabolites of Human Vascular Endothelial Cells Altered by Cyclooxygenase Inhibition, Growth Factor Depletion, and Paracrine Factors Secreted by Cancer Cells
Authors: Mori Noriko , Natarajan Kshama , Chacko V. P. , Artemov Dmitri , Bhujwalla Zaver M.
Published: March 2018

Magnetic resonance studies have previously shown that solid tumors and cancer cells in culture typically exhibit high phosphocholine and total choline. Treatment of cancer cells with the anti-inflammatory agent, indomethacin (INDO), reverted the phenotype of choline phospholipid metabolites in cancer cells towards a less malignant phenotype. Since endothelial cells form a key component of tumor vasculature, in this study, we used MR spectroscopy to characterize the phenotype of choline phospholipid metabolites in human umbilical vein endothelial cells (HUVECs). We determined the effect of growth factors, the anti-inflammatory agent INDO, and conditioned media obtained from a malignant cell line, on choline phospholipid metabolites. Growth factor depletion or treatment with INDO induced similar changes in the choline phospholipid metabolites of HUVECs. Treatment with conditioned medium obtained from MDA-MB-231 cancer cells induced changes similar to the presence of growth factor supplements. These results suggest that cancer cells secrete growth factors and/or other molecules that influence the choline phospholipid metabolism of HUVECs. The ability of INDO to alter choline phospholipid metabolism in the presence of growth factor supplements suggests that the inflammatory response pathways of HUVECs may play a role in cancer cell-HUVEC interaction and in the response of HUVECs to growth factors.
Breaching Biological Barriers: Protein Translocation Domains as Tools for Molecular Imaging and Therapy
Authors: Franc Benjamin L. , Mandl Stefanie J. , Siprashvili Zurab , Wender Paul , Contag Christopher H.
Published: March 2018

The lipid bilayer of a cell presents a significant barrier for the delivery of many molecular imaging reagents into cells at target sites in the body. Protein translocation domains (PTDs) are peptides that breach this barrier. Conjugation of PTDs to imaging agents can be utilized to facilitate the delivery of these agents through the cell wall, and in some cases, into the cell nucleus, and have potential for in vitro and in vivo applications. PTD imaging conjugates have included small molecules, peptides, proteins, DNA, metal chelates, and magnetic nanoparticles. The full potential of the use of PTDs in novel in vivo molecular probes is currently under investigation. Cells have been labeled in culture using magnetic nanoparticles derivatized with a PTD and monitored in vivo to assess trafficking patterns relative to cells expressing a target antigen. In vivo imaging of PTD-mediated gene transfer to cells of the skin has been demonstrated in living animals. Here we review several natural and synthetic PTDs that have evolved in the quest for easier translocation across biological barriers and the application of these peptide domains to in vivo delivery of imaging agents.
Quantitative Comparison of the Sensitivity of Detection of Fluorescent and Bioluminescent Reporters in Animal Models
Authors: Troy Tamara , Jekic-McMullen Dragana , Sambucetti Lidia , Rice Brad
Published: March 2018

Bioluminescent and fluorescent reporters are finding increased use in optical molecular imaging in small animals. In the work presented here, issues related to the sensitivity of in vivo detection are examined for standard reporters. A high-sensitivity imaging system that can detect steady-state emission from both bioluminescent and fluorescent reporters is described. The instrument is absolutely calibrated so that animal images can be analyzed in physical units of radiance allowing more quantitative comparisons to be performed. Background emission from mouse tissue, called autoluminescence and autofluorescence, is measured and found to be an important limitation to detection sensitivity of reporters. Measurements of dual-labeled (bioluminescent/fluorescent) reporter systems, including PC-3M-luc/DsRed2-1 and HeLa-luc/PKH26, are shown. The results indicate that although fluorescent signals are generally brighter than bioluminescent signals, the very low autoluminescent levels usually results in superior signal to background ratios for bioluminescent imaging, particularly compared with fluorescent imaging in the green to red part of the spectrum. Fluorescence detection sensitivity improves in the far-red to near-infrared, provided the animals are fed a low-chlorophyll diet to reduce autofluorescence in the intestinal region. The use of blue-shifted excitation filters is explored as a method to subtract out tissue autofluorescence and improve the sensitivity of fluorescent imaging.
Cardiac-Specific Gene Expression Facilitated by an Enhanced Myosin Light Chain Promoter
Authors: Boecker Wolfgang , Bernecker Oliver Y. , Wu Joseph C. , Zhu Xinsheng , Sawa Tomohiro , Grazette Luanda , Rosenzweig Anthony , del Monte Federica , Schmidt Ulrich , Hajjar Roger J.
Published: March 2018

Background: Adenoviral gene transfer has been shown to be effective in cardiac myocytes in vitro and in vivo. A major limitation of myocardial gene therapy is the extracardiac transgene expression. Methods: To minimize extracardiac gene expression, we have constructed a tissue-specific promoter for cardiac gene transfer, namely, the 250-bp fragment of the myosin light chain-2v (MLC-2v) gene, which is known to be expressed in a tissue-specific manner in ventricular myocardium followed by a luciferase (luc) reporter gene (Ad.4 × MLC250.Luc). Rat cardiomyocytes, liver and kidney cells were infected with Ad.4 × MLC.Luc or control vectors. For in vivo testing, Ad.4 × MLC250.Luc was injected into the myocardium or in the liver of rats. Kinetics of promoter activity were monitored over 8 days using a cooled CCD camera. Results: In vitro: By infecting hepatic versus cardiomyocyte cells, we found that the promoter specificity ratio (luc activity in cardiomyocytes per liver cells) was 20.4 versus 0.9 (Ad.4 × MLC250.Luc vs. Ad.CMV). In vivo: Ad.4 × MLC250.Luc significantly reduced luc activity in liver (38.4-fold), lung (16.1-fold), and kidney (21.8-fold) versus Ad.CMV ( p = .01); whereas activity in the heart was only 3.8-fold decreased. The gene expression rate of cardiomyocytes versus hepatocytes was 7:1 (Ad.4 × MLC.Luc) versus 1:1.4 (Ad.CMV.Luc). Discussion: This new vector may be useful to validate therapeutic approaches in animal disease models and offers the perspective for selective expression of therapeutic genes in the diseased heart.
Radiation-Force Assisted Targeting Facilitates Ultrasonic Molecular Imaging
Authors: Zhao Shukui , Borden Mark , Bloch Susannah H. , Kruse Dustin , Ferrara Katherine W. , Dayton Paul A.
Published: March 2018

Ultrasonic molecular imaging employs contrast agents, such as microbubbles, nanoparticles, or liposomes, coated with ligands specific for receptors expressed on cells at sites of angiogenesis, inflammation, or thrombus. Concentration of these highly echogenic contrast agents at a target site enhances the ultrasound signal received from that site, promoting ultrasonic detection and analysis of disease states. In this article, we show that acoustic radiation force can be used to displace targeted contrast agents to a vessel wall, greatly increasing the number of agents binding to available surface receptors. We provide a theoretical evaluation of the magnitude of acoustic radiation force and show that it is possible to displace micron-sized agents physiologically relevant distances. Following this, we show in a series of experiments that acoustic radiation force can enhance the binding of targeted agents: The number of biotinylated microbubbles adherent to a synthetic vessel coated with avidin increases as much as 20-fold when acoustic radiation force is applied; the adhesion of contrast agents targeted to αvβ3 expressed on human umbilical vein endothelial cells increases 27-fold within a mimetic vessel when radiation force is applied; and finally, the image signal-to-noise ratio in a phantom vessel increases up to 25 dB using a combination of radiation force and a targeted contrast agent, over use of a targeted contrast agent alone.
Small-Animal X-ray Dose from Micro-CT
Authors: Boone John M. , Velazquez Orlando , Cherry Simon R.
Published: March 2018

The use of micro-CT in small animals has increased in recent years. Although the radiation levels used for micro-CT are generally not lethal to the animal, they are high enough where changes in the immune response and other biological pathways may alter the experimental outcomes. Therefore, it is important to understand what the doses are for a specific imaging procedure. Monte Carlo simulation was used to evaluate the radiation dose to small animals (5–40 mm in diameter) as a result of X-ray exposure. Both monoenergetic (6–100 keV) and polyenergetic (15–100 kVp) X-ray sources were simulated under typical mouse imaging geometries. X-ray spectral measurements were performed on a mouse imaging X-ray system using a commercially available X-ray spectrometer, and spectra from high-energy systems were used as well. For a typical X-ray system with 1.0 mm of added Al at 40 kVp, the dose coefficients (dose to mouse per air kerma at isocenter) were 0.80, 0.63, 0.52, and 0.44 mGy/mGy for mouse diameters of 10, 20, 30, and 40 mm, respectively. A number of tables and figures are provided for dose estimation over a range of mouse imaging geometries.
In Vivo Imaging of Retinoic Acid Receptor Activity using a Sodium/Iodide Symporter and Luciferase Dual Imaging Reporter Gene
Authors: So Min Kyung , Kang Joo Hyun , Chung June-Key , Lee Yong Jin , Shin Jae Hoon , Kim Kwang Il , Jeong Jae Min , Lee Dong Soo , Lee Myung Chul
Published: March 2018

Retinoic acids are natural derivatives of vitamin A, and play important roles in modulating tumor cell growth by regulating differentiation, thus suggesting the potential use of these derivatives in cancer therapy and prevention. To visualize the intranuclear responses of functional retinoic acid receptors, we have developed a dual-imaging reporter gene system based on the use of sodium/iodide symporter (NIS) and luciferase in cancer cell lines. NIS and luciferase genes were linked with an internal ribosome entry site, and placed under the control of an artificial cis-acting retinoic acid responsive element (pRARE/NL). After retinoic acid treatment, I-125 uptake by pRARE/NL transfected cells was found to have increased by up to about five times that of nontreated cells. The bioluminescence intensity of pRARE/NL transfected cells showed dose-dependency. In vivo luciferase images showed higher intensity in retinoic acid treated SK-RARE/NL tumors, and scintigraphic images of SK-RARE/NL tumors showed increased Tc-99m uptake after retinoic acid treatment. The NIS/luciferase imaging reporter system was sufficiently sensitive to allow the visualization of intranuclear retinoic acid receptor activity. This cis-enhancer imaging reporter system may be useful in vitro and in vivo for the evaluation of retinoic acid responses in such areas as cellular differentiation and chemoprevention.
Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation
Authors: Crich Simonetta Geninatti , Lanzardo Stefania , Barge Alessandro , Esposito Giovanna , Tei Lorenzo , Forni Guido , Aime Silvio
Published: March 2018

The ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, Ka = 2.2 × 103 M−1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.
A Potential Dubin-Johnson Syndrome Imaging Agent: Synthesis, Biodistribution, and MicroPET Imaging
Authors: Yoo Jeongsoo , Reichert David E. , Kim Joonyoung , Anderson Carolyn J. , Welch Michael J.
Published: March 2018

Dubin-Johnson syndrome (DJS) is caused by a deficiency of the human canalicular multispecific organic anion transporter (cMOAT). A new lipophilic copper-64 complex of 1,4,7-tris(carboxymethyl)-10-(tetradecyl)-1,4,7,10-tetraazadodecane (5) was prepared and evaluated for potential as a diagnostic tool for DJS. The prepared ligand was labeled with 64Cu citrate in high radiochemical purity. In vivo uptake and clearance of the complex was determined through biodistribution studies using normal Sprague-Dawley rats and mutant cMOAT-deficient (TR−) rats. In normal rats, the radioactive copper complex was cleared quickly from the body exclusively through the hepatic pathway. The 64Cu complex was taken up rapidly by the liver and quickly excreted into the small intestine and then the upper large intestine, whereas < 1% ID/organ was found in the kidney at all time points post injection. Whereas activity was accumulated continuously in the liver of TR− rats, it was not excreted into the small intestine. MicroPET studies of normal and TR rats were consistent with biodistribution data and showed dramatically different images. This study strongly suggests that cMOAT is involved in excretion of 64Cu-5. The significant difference between the biodistribution data and microPET images of the normal and TR− rats demonstrates that this new 64Cu complex may allow noninvasive diagnosis of DJS in humans.
Cellular Imaging of Inflammation after Experimental Spinal Cord Injury
Authors: Dunn Elizabeth A. , Weaver Lynne C. , Dekaban Gregory A. , Foster Paula J.
Published: March 2018

The ability to visualize the cellular inflammatory responses after experimental spinal cord injury (SCI) was investigated using a clinical 1.5-T magnetic resonance imaging scanner, a custom-built, high-strength gradient coil insert, a 3-D fast imaging employing steady-state acquisition (FIESTA) imaging sequence and a superparamagnetic iron oxide (SPIO) contrast agent. An “active labeling” approach was used, with SPIO administered intravenously at different time points following SCI. Our results show that this strategy can be used to visualize clusters of iron-labeled cells associated with the inflammatory response in SCI. Of particular importance for this application was the finding that in FIESTA images hemorrhage does not cause signal loss. In T2-weighted spin echo or T2*-weighted gradient-echo images, which are more commonly used to detect signal loss associated with SPIO, the signal loss associated with hemorrhage interferes with the detection of iron-induced signal loss. FIESTA, therefore, allowed us to discriminate between iron associated with blood products in hemorrhage that occurs in acute SCI and the iron associated with SPIO-labeled cells accumulating in the injured cord.
Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas
Authors: Himmelreich Uwe , Weber Ralph , Ramos-Cabrer Pedro , Wegener Susanne , Kandal Korinna , Shapiro Eric M. , Koretsky Alan P. , Hoehn Mathias
Published: March 2018

In vivo monitoring of cells labeled with paramagnetic iron oxide particles by magnetic resonance imaging (MRI) is complicated by intrinsic contrast of blood vessels. Distinction between T2* effects caused by blood vessels from those caused by labeled cells was so far only possible after carefully following the location of hypointense regions through subsequent slices of T2*-weighted 3-D MRI datasets, a procedure that is time consuming and not always reliable in the case of smaller blood vessels. Here, we demonstrate that the modification of the inhalation gas mixture from the routinely used composition 35% O2 and 65% N2O to a mixture containing 95% O2 and 5% CO2 results in a contrast suppression of most small blood vessels and reduces the intrinsic T2* effect of large vessels dramatically in an animal model. This change in protocol of physiological conditions was well tolerated by all studied animals, even over prolonged experimental times. The changed inhalation gas mixture thus provides a more reliable identification method for small clusters of iron oxide labeled cells in vivo.
Noninvasive Monitoring of Pneumococcal Meningitis and Evaluation of Treatment Efficacy in an Experimental Mouse Model
Authors: Kadurugamuwa Jagath L. , Modi Kshitij , Yu Jun , Francis Kevin P. , Orihuela Carlos , Tuomanen Elaine , Purchio Anthony F. , Contag Pamela R.
Published: March 2018

Noninvasive real-time in vivo bioluminescent imaging was used to assess the spread of Streptococcus pneumoniae throughout the spinal cord and brain during the acute stages of bacterial meningitis. A mouse model was established by lumbar (LP) or intracisternal (IC) injection of bioluminescent S. pneumoniae into the subarachnoid space. Bacteria replicated initially at the site of inoculation and spread progressively from the spinal cord to the brain or from the brain down to the cervical part of the spinal column and to the lower vertebral levels. After 24 hr, animals showed strong bioluminescent signals throughout the spinal canal, indicating acute meningitis of the intracranial and intraspinal meninges. A decline in bacterial cell viability, as judged by a reduction in the bioluminescent signal, was observed over time in animals treated with ceftriaxone, but not in untreated groups. Mice treated with the antibiotic survived infection, whereas all mice in untreated groups became moribund, first in the IC group then in the LP group. No untreated animal survived beyond 48 hr after induction of infection. Colony counts of infected cerebrospinal fluid (CSF) correlated positively with bioluminescent signals. This methodology is especially appealing because it allows detecting infected mice as early as 3 hr after inoculation, provide temporal, sequential, and spatial distribution of bacteria within the brain and spinal cord throughout the entire disease process and the rapid monitoring of treatment efficacy in a nondestructive manner. Moreover, it avoids the need to sacrifice the animals for CSF sampling and the potential manipulative damage that can occur with other conventional methods.
Improved Excitation Light Rejection Enhances Small-Animal Fluorescent Optical Imaging
Authors: Hwang Kildong , Houston Jessica P. , Rasmussen John C. , Joshi Amit , Ke Shi , Li Chun , Sevick-Muraca Eva M.
Published: March 2018

Small-animal fluorescence-enhanced imaging involves the detection of weak fluorescent signals emanating from nanomolar to picomolar concentrations of exogenous or endogenously produced fluorophore concurrent with the rejection of an overwhelmingly large component of backscattered excitation light. The elimination of the back-reflected excitation light of the collected signal remains a major and often unrecognized challenge for further reducing the noise floor and increasing sensitivity of small-animal fluorescence imaging. Herein, we show that the combination of three-cavity interference and holographic super notch filters with appropriate imaging lenses to collimate light improves rejection of excitation light, enabling more accurate imaging. To assess excitation leakage, the “out-of-band ( S(Λ x))” to “in-band ( S(Λ m)– S(Λ x))” signal ratio from phantom studies and the target-to-background ratio (TBR) from in vivo animal imaging was acquired with and without collimating optics. The addition of collimating optics resulted in a 51% to 75% reduction in the ratio of ( S(Λ x))/( S(Λ m)– S(Λ x)) for the phantom studies and an improvement of TBR from 11% to 31% and of signal-to-noise ratio from 11% to 142% for an integrin-targeting conjugate in human glioma xenografts.
Muḥammad as an Episcopal Figure
Authors: Sinai Nicolai
Published: March 2018

The Medinan stratum of the Qurʾān ascribes to Muḥammad a noticeably elevated status and a far wider range of functions than the earlier Meccan layer. Although this shift may well have responded to, and been facilitated by, historical circumstances, it is nonetheless appropriate to inquire whether specific aspects of it might be drawing on pre-Qurʾānic precedents. I argue that the Christian episcopate, arguably the most widespread type of urban religious leadership in late antiquity, yields a surprising number of close overlaps with the Medinan presentation of the function and authority of Muḥammad. In tandem with this assessment, however, the article also considers important differences between the figure of Muḥammad and that of the Christian bishop. The most important such divergence consists in the fact that the Qurʾānic Messenger, unlike a Christian bishop, does not owe his authority to ordination by an ecclesiastical hierarchy: Muḥammad does not occupy an office that imparts authority independently of the person occupying it.
Spectral sensitivities of ants – a review
Authors: Aksoy Volkan , Camlitepe Yilmaz
Published: March 2018

Ants constitute one of the most intriguing animal groups with their advanced social lifes, different life histories and sensory modalities, one of which is vision. Chemosensation dominates all other modalities in the accomplishment of different vital tasks, but vision, varying from total blindness in some species to a relatively well-developed vision providing ants the basis for visually-guided behaviors, is also of importance. Although studies on ant vision mainly focused on recognition of and guidance by landmark cues in artificial and/or natural conditions, spectral sensitivities of their compound eyes and ocelli were also disclosed, but to a lesser extent. In this review, we have tried to present current data on the spectral sensitivities of the different ant species tested so far and the different methodological approaches. The results, as well as the similarities and/or discrepancies of the methodologies applied, were compared. General tendencies in ants’ spectral sensitivities are presented in a comparative manner and the role of opsins and ant ocelli in their spectral sensitivity is discussed in addition to the sensitivity of ants to long wavelengths. Extraocular sensitivity was also shown in some ant species. The advantages and/or disadvantages of a dichromatic and trichromatic color vision system are discussed from an ecological perspective.
Intact Dynamic Visual Capture in People With One Eye
Authors: Moro Stefania S. , Steeves Jennifer K. E.
Published: March 2018

Observing motion in one modality can influence the perceived direction of motion in a second modality (dynamic capture). For example observing a square moving in depth can influence the perception of a sound to increase in loudness. The current study investigates whether people who have lost one eye are susceptible to audiovisual dynamic capture in the depth plane similar to binocular and eye-patched viewing control participants. Partial deprivation of the visual system from the loss of one eye early in life results in changes in the remaining intact senses such as hearing. Linearly expanding or contracting discs were paired with increasing or decreasing tones and participants were asked to indicate the direction of the auditory stimulus. Magnitude of dynamic visual capture was measured in people with one eye compared to eye-patched and binocular viewing controls. People with one eye have the same susceptibility to dynamic visual capture as controls, where they perceived the direction of the auditory signal to be moving in the direction of the incongruent visual signal, despite previously showing a lack of visual dominance for audiovisual cues. This behaviour may be the result of directing attention to the visual modality, their partially deficient sense, in order to gain important information about approaching and receding stimuli which in the former case could be life-threatening. These results contribute to the growing body of research showing that people with one eye display unique accommodations with respect to audiovisual processing that are likely adaptive in each unique sensory situation.
Mesostigmatic mites (Acari: Mesostigmata) of Saudi Arabia (excluding Phytoseioidea), new records and a key to the known species
Authors: ALATAWI FAHAD JABER , KAMRAN MUHAMMAD , MIRZA JAWWAD HASSAN
Published: March 2018

This paper reports on 35 species and 33 genera belonging to 18 families of the order Mesostigmata, collected from different provinces of Saudi Arabia. Among these, eight families, 21 genera and 30 species, mostly collected from date palm agroecosystem, are new to the fauna of Saudi Arabia. Five species reported previously are also recorded in this study. The collection data and distribution of all species recorded in this study as well as those reported previously are given. A comprehensive key to the females of the mesostigmatic mites reported from Saudi Arabia, except the Phytoseioidea, is provided.
First record of Africactenus Hyatt, 1954 and redescriptions of two poorly known species of Ctenus Walckenaer, 1805 (Araneae, Ctenidae, Cteninae) from India
Authors: SANKARAN PRADEEP M. , SEBASTIAN POTHALIL A.
Published: March 2018

The ctenid genus Africactenus Hyatt, 1954 is recorded for the first time from India. Africactenus unumus sp. nov. is described and illustrated based on male specimens. Detailed redescriptions and illustrations of Ctenus cochinensis Gravely, 1931 (both male and female) and Ctenus indicus Gravely, 1931 (only female) are provided and their distribution in India is updated.
Observations in Aid of Weather Prediction for North America: Report of Prospectus Development Team Seven
Authors: Emanuel Kerry , Kalnay Eugenia , Bishop Craig , Elsberry Russell , Gelaro Ronald , Keyser Daniel , Lord Steven , Rogers David , Shapiro Melvyn , Snyder Christopher , Velden Christopher
Published: March 2018

One of the most significant impediments to progress in forecasting weather over North America is the relative paucity of routine observations over data-sparse regions adjacent to the United States. Prospectus Development Team Seven was convened to consider ways to promote research that seeks to determine implementations of observing systems that are optimal for weather prediction in the United States. An “optimal” measurement system is considered to be one that maximizes the ratio of societal benefit to overall cost. The thrust of the conclusions is that existing means of estimating the value of current observing systems and the potential benefits of new or proposed observing systems are underutilized. At the same time, no rational way exists for comparing the cost of observations across the spectrum of federal agencies responsible for measuring the atmosphere and ocean. The authors suggest that a rational procedure for configuring an observation system that is optimal for weather prediction would consist of the following steps.Identify specific forecast problems arising from insufficient data. Examples might include hurricane landfall and intensity forecasts, 24-h forecasts of intense extratropical cyclones affecting the West Coast and Alaska, and medium-range forecasts of severe weather for all of North America.Use contemporary modeling techniques, such as observing system simulation experiments, ensemble forecasting, and model adjoint-derived sensitivities, to delineate measurement requirements for each specific forecasting problem and identify candidate observing systems and data assimilation techniques that could be brought to bear on each problem.Estimate the incremental forecast improvements that could plausibly result from the added or reconfigured data and the societal benefits that would accrue from such improvements.Estimate the overall cost (to the nation, not to specific federal agencies) of obtaining the data by the various candidate techniques and the benefits that are projected to result.Use standard cost–benefit analysis as a basis for deciding the optimal deployment of measuring systems.

The authors believe that a rational approach to atmospheric measurement is critical to further improvements in weather prediction and that such improvements might very well be made within the current budget of routine observations, integrated across all of the responsible federal agencies. This document outlines a proposed strategy for rationalizing atmosphere observations in aid of weather prediction in the United States. The paper begins with a summary of recommendations.
Design and Test of a “Hands-On” Applied Climate Course in an Undergraduate Meteorology Program
Authors: Changnon David
Published: March 2018

Job opportunities for undergraduate meteorology students are decreasing. An innovative course in applied climatology has been designed and tested to help prepare such students for the career options developing in the private sector. Students are trained to use their meteorological knowledge and analytical skills to work interactively with weather-sensitive users in utilities, agribusinesses, water-resource agencies, recreation firms, and transportation companies. The students develop and test climate relationship-decision models in a real-world environment for these organizations. The models they develop bridge existing information “gaps” between climatologists and weather-sensitive managers who 1) do not understand climate information, and/or 2) do not know how to apply it to their environmental or economic decisions. As a result, students receive applied research experience and important “education-to-career” opportunities; that is, students can apply what is learned through direct and often beneficial interactions with decision makers. These efforts address problems similar to those they likely will encounter after employment. Other long-term objectives of this course are to develop a more effective information flow between climatologists and weather-sensitive users and to assist climatologists by identifying the types of needs for climate information.
Observations for Reanalyses
Authors: Brönnimann Stefan , Allan Rob , Atkinson Christopher , Buizza Roberto , Bulygina Olga , Dahlgren Per , Dee Dick , Dunn Robert , Gomes Pedro , John Viju O. , Jourdain Sylvie , Haimberger Leopold , Hersbach Hans , Kennedy John , Poli Paul , Pulliainen Jouni , Rayner Nick , Saunders Roger , Schulz Jörg , Sterin Alexander , Stickler Alexander , Titchner Holly , Valente Maria Antonia , Ventura Clara , Wilkinson Clive
Published: March 2018

Global dynamical reanalyses of the atmosphere and ocean fundamentally rely on observations, not just for the assimilation (i.e., for the definition of the state of the Earth system components) but also in many other steps along the production chain. Observations are used to constrain the model boundary conditions, for the calibration or uncertainty determination of other observations, and for the evaluation of data products. This requires major efforts, including data rescue (for historical observations), data management (including metadatabases), compilation and quality control, and error estimation. The work on observations ideally occurs one cycle ahead of the generation cycle of reanalyses, allowing the reanalyses to make full use of it. In this paper we describe the activities within ERA-CLIM2, which range from surface, upper-air, and Southern Ocean data rescue to satellite data recalibration and from the generation of snow-cover products to the development of a global station data metadatabase. The project has not produced new data collections. Rather, the data generated has fed into global repositories and will serve future reanalysis projects. The continuation of this effort is first contingent upon the organization of data rescue and also upon a series of targeted research activities to address newly identified in situ and satellite records.
Why Groups Are Politically Active: An Incentive-Theoretical Approach
Authors: Bolleyer Nicole , Weiler Florian
Published: March 2018

Political activity is conventionally considered a constitutive feature of interest groups, underpinning an impressive literature on the strategies groups employ to exercise political influence. Whether and how intensely voluntary membership groups engage in political activities to start with, however, are rarely examined. We present a new incentive-theoretical perspective on group political activity, considering both member demands and leadership constraints. We argue that investments in political activities (one way of generating collective incentives) as a means to prevent member exit are more or less important depending on a group’s composition. Simultaneously, the extent to which leaders are incentivized to cater to members’ demands, when trying to balance these against conflicting demands, depends on communication channels between leaders and members and the importance of membership fees. Applying Bayesian ordered logit models to data from two group surveys supports our perspective and stresses the importance of considering how intraorganizational dynamics steer groups’ external activities.
Creaming and parking in marketized employment services: An Anglo-German comparison
Authors: Greer Ian , Schulte Lisa , Symon Graham
Published: March 2018

The delivery of public services by nonprofit and for-profit providers alters the nature of services and jobs, often in unintended and undesired ways. We argue that these effects depend on the degree to which the service is ‘marketized’, that is, subjected by the funder to price-based competition. Using case studies of British and German employment services, this article scrutinizes the link between funding practices and service quality. Of particular concern in marketized employment services is the problem of ‘creaming and parking’, in which providers select job-ready clients for services and neglect clients more distant from the labour market. We explore three questions. What are the mechanisms through which marketization produces creaming and parking? What are the differences between these mechanisms in commercial and non-commercial service providers? Which national institutions might serve as a buffer for the landscape of service provision facing price-based competition?
Managing the Unmanageable
Authors: Lowerson Ashley Jane
Published: March 2018

This article analyses the current context and the use of the public order offence of ‘riot’ held in s. 1 of the Public Order Act 1986. It examines the historical roots of the statutory offence and the difficulty of securing a conviction following times of public disorder due to the nature and interpretation of the wording in s. 1. The article identifies, and uses as an example, the perception that football disorder includes riotous football fans, although official Home Office statistics on football disorder highlight that the offence of riot is not utilised. The statutory offence of ‘riot’ is an underused tool, although the term ‘riot’ is freely used by the media to describe disorderly behaviour. The article projects to make a recommendation that the factors underpinning the definition of riot needs to be re-examined to enable the judiciary to interpret s. 1 in a manner that is more practicable.
Responses to the Statements of New General Self-Efficacy Scale: The Case of the Arabic–English Bilingual Speaker
Authors: Mulhem Huda , El Alaoui Khadija , Hamdan Amani K. , Abdul-Rahim Mohammad B. , Pilotti Maura A. E. , Tallouzi Ebtesam A.
Published: March 2018

General Self-Efficacy (GSE) scales are used to measure the extent to which individuals possess a general sense of mastery that is not tied to a specific situation or behavior. The present field experiment assesses whether the language in which a GSE scale is written can prime cultural mind-sets and, thus, shape participants’ appraisal of their own self-efficacy. Arabic–English bilingual speakers completed the New General Self-Efficacy (NGSE) scale and a questionnaire to measure their emotional reactions to each of the statements of the scale. Across all participants, Arabic yielded lower self-efficacy appraisals than English. Furthermore, in Arabic, both males’ and females’ reports of positive emotions decreased as self-efficacy estimates increased, whereas in English, females’ reports of positive emotions rose with self-efficacy estimates. These results support the notion that self-appraisal of mastery can be modulated by one’s cultural mind-set.
Activation of Stimulator of Interferon Genes (STING) and Sjögren Syndrome
Authors: Papinska J. , Bagavant H. , Gmyrek G.B. , Sroka M. , Tummala S. , Fitzgerald K.A. , Deshmukh U.S.
Published: March 2018

Sjögren syndrome (SS), a chronic autoimmune disorder causing dry mouth, adversely affects the overall oral health in patients. Activation of innate immune responses and excessive production of type I interferons (IFNs) play a critical role in the pathogenesis of this disorder. Recognition of nucleic acids by cytosolic nucleic acid sensors is a major trigger for the induction of type I IFNs. Upon activation, cytosolic DNA sensors can interact with the stimulator of interferon genes (STING) protein, and activation of STING causes increased expression of type I IFNs. The role of STING activation in SS is not known. In this study, to investigate whether the cytosolic DNA sensing pathway influences SS development, female C57BL/6 mice were injected with a STING agonist, dimethylxanthenone-4-acetic acid (DMXAA). Salivary glands (SGs) were studied for gene expression and inflammatory cell infiltration. SG function was evaluated by measuring pilocarpine-induced salivation. Sera were analyzed for cytokines and autoantibodies. Primary SG cells were used to study the expression and activation of STING. Our data show that systemic DMXAA treatment rapidly induced the expression of Ifnb1, Il6, and Tnfa in the SGs, and these cytokines were also elevated in circulation. In contrast, increased Ifng gene expression was dominantly detected in the SGs. The type I innate lymphoid cells present within the SGs were the major source of IFN-γ, and their numbers increased significantly within 3 d of treatment. STING expression in SGs was mainly observed in ductal and interstitial cells. In primary SG cells, DMXAA activated STING and induced IFN-β production. The DMXAA-treated mice developed autoantibodies, sialoadenitis, and glandular hypofunction. Our study demonstrates that activation of the STING pathway holds the potential to initiate SS. Thus, apart from viral infections, conditions that cause cellular perturbations and accumulation of host DNA within the cytosol should also be considered as possible triggers for SS.
Regulating and resisting queer creativity: Community-engaged arts practice in the neoliberal city
Authors: McLean Heather
Published: March 2018

This article draws from and advances urban studies literature on ‘creative city’ policies by exploring the contradictory role of queer arts practice in contemporary placemarketing strategies. Here I reflect on the fraught politics surrounding Radiodress’s each hand as they are called project, a deeply personal exploration of radical Jewish history programmed within Luminato, a Toronto-based international festival of creativity. Specifically, I explore how Luminato and the Koffler Centre, a Jewish organisation promoting contemporary art, regulated Radiodress’s work in order to stage marketable notions of ethnic and queer diversity. I also examine how and why the Koffler Centre eventually blacklisted Radiodress and her project. However, I also consider the ways Radiodress and Toronto artists creatively and collectively responded to these tensions. I maintain that bringing queer arts practice into discussions about contemporary creative city policies uncovers sites of queer arts activism that scale up to shape broader policies and debates. Such disidentificatory interventions, acts of co-opting and re-working discourses which exclude minoritarian subjects, challenge violent processes of colonisation and commodification on multiple fronts, as well as fostering more collective and relational ways of being.