Development of endogenic intoxication in rats under nitrite tobacco
toxicosis after carboline application
Authors: Lykhatskyi Petro H. , Fira V.D. , Fira PLudmila S. , Boyko L.A. 
Published: March 2024

Summary. Today, more and more attention of researchers is paid to the
combined pathologies that may be due to the action of several toxic factors
on the body. Interest in elucidating the mechanisms of action of nitrites
and nitrates on the body is due to their widespread use in industry,
agriculture and medicine. Smoking is a socio-economic phenomenon and one of
the most important health problems in Ukraine and around the world, as it is
accessible to all and therefore widespread. Intake of exogenous toxicants is
accompanied by the development of oxidative stress and the accumulation of
toxic products in various organs, the deepening of endogenous intoxication,
to eliminate which modern enterosorbents are used. The aim of the study – to
determine the degree of endogenous intoxication in rats of different ages
under conditions of nitrite-tobacco toxicosis after application of the
enterosorbent carboline. Materials and Methods. The experiments were
performed on white male rats of different ages that were exposed to tobacco
smoke for 45 days and received sodium nitrite 24 hours and 72 hours before
the end of the study. One of the groups of rats on the background of toxic
poisoning was administered enterosorbent carboline at a dose of 400 mg/kg of
body weight. Animals were removed from the experiment on the 30th and 45th
day of tobacco intoxication (and after 24 and 72 hours of sodium nitrite
poisoning). Results. A study of the content of MetHb in the blood of rats
affected by sodium nitrite on the background of tobacco intoxication, showed
a progressive increase in all age groups during the experiment. The most
pronounced increase in the permeability of erythrocyte membranes was
observed in immature rats during the experiment. In the last period of the
experiment, this figure increased by 55.6 % in this group of animals. The
indicators in senile animals were almost at the same level. The
enterosorbent carboline was effective in methemoglobin and erythrocyte
intoxication index, reducing these rates in poisoned animals compared to
rats that did not receive it. In the experiment, after poisoning of rats of
different ages with sodium nitrite on the background of tobacco intoxication
in the serum, an increase in the content of MMM of both fractions – MM1 and
MM2. Immature rats were more sensitive to the content of endogenous toxins.
Throughout the experiment, they had the highest content of medium weight
molecules in the serum compared to adult and senile rats. The 30-day use of
carboline was effective for all age groups. Conclusions. Under conditions of
nitrite-tobacco toxicosis in animals of different ages deepens endogenous
intoxication, manifested by increased methemoglobin and erythrocyte membrane
permeability, as well as increased content of medium weight molecules in the
serum of rats during 45 days of intoxication with tobacco poisoning.
Immature rats were the most sensitive to toxicants. The enterosorbent
carboline used under these conditions probably reduced all indicators of
endogenous intoxication in rats of all ages