bluuebunny/crossref_metadata_2025 huggingface dataset:
MECANISMOS DE CREACIÓN DE IDENTIDAD EN EL DISEÑO DE PRODUCTOS A TRAVÉS DE VALORES CULTURALES, EXPERIENCIALES Y RACIONALES
Authors: Martínez Rodríguez Rodrigo , Fernández-Gago Paula
Published: June 2024

El presente artículo explora el fenómeno del consumo identitario y la importancia de considerar, en el diseño de producto, las distintas posibilidades de inclusión de valores de distinta índole. Se aleja de una visión exclusiva del diseño como una disciplina configuradora con base en el binomio forma-función. Este trabajo expone la relevancia de dotar de identidad a los productos y, con ello, la posibilidad de creación de significados, al profundizar en la clasificación de valores de consumo- culturales, experienciales y racionales-. Esto se hace a través de ejemplos y casos prácticos que demuestran la importancia de emplear la identidad como un activo importante de consumo.Palabras clave: diseño de producto, identidad, valores de consumo, diseño y significado. 
AbstractThis article explores the phenomenon of identity consumption and the importance of considering the different possibilities of including values of different kinds in product design. It moves away from an exclusive vision of design as a shaping discipline based on the form-function binomial. This work exposes the relevance of providing identity to products and, with it, the possibility of creating meanings, delving into the classification of consumer values -cultural, experiential, and rational-, through examples and practical cases that demonstrate the importance of employ identity as an important consumer asset.
Keywords: product design, identity, consumption values, design and meaning.
Innovación y participación social para un enfoque colaborativo de la gestión patrimonial
Authors: Carrera Díaz Gema
Published: June 2024

El proyecto LAPat: Laboratorio Abierto del Patrimonio Cultural de Andalucía (PAIDI, 2018) busca transformar la gestión patrimonial mediante metodologías participativas e innovación social. Centrado en diversos ámbitos como documentación, difusión y restauración, el proyecto plantea como objetivos fundamentales fortalecer el papel mediador de las instituciones, recopilar iniciativas ciudadanas de innovación social, generar herramientas participativas y colaborar en la creación de contenidos digitales accesibles sobre el patrimonio cultural. Se busca consolidar así la Red de Agentes Informantes del Patrimonio Cultural y el papel del IAPH como Laboratorio Abierto de Patrimonio, promoviendo la transparencia institucional y la colaboración con diferentes agentes. Los casos de estudio presentados muestran el impacto de estas metodologías en la reactivación urbana, la generación de conocimiento colectivo y la promoción del patrimonio cultural. Este proyecto responde al desafío de construir sociedades inclusivas, innovadoras y reflexivas, en línea con el Plan Andaluz de Investigación y Desarrollo.
Pengembangan Digitalisasi Pemasaran UMKM Pada Kelompok Pengolah dan Pemasar Ikan Waluya Kota Bandar Lampung
Authors: Ramadhani Mutiara Aziza
Published: June 2024

Hadirnya media sosial sangat penting untuk meningkatkan bisnis dengan mempromosikan produknya khususnya untuk para pelaku bisnis kecil atau Usaha Mikro, Kecil, dan Menengah (UMKM). Pemasaran konvensional masih menjadi kendala utama yang harus diperhatikan pemerintah agar UMKM dapat menjangkau pasar lebih luas. Banyak UMKM belum memanfaatkan internet melalui media sosial atau e-Commerce untuk usaha mereka. Peserta kegiatan ini merupakan UMKM Pengrajin Ikan Teri Pulau Pasaran Provinsi Lampung bernama Kelompok Pengolah dan Pemasar Ikan (Poklahsar) Waluya. Kegiatan diikuti sebanyak 6 peserta, terdiri dari ketua dan tim inti kelompok usaha yang terlibat dalam pengembangan usaha. Kegiatan ini menggunakan metode Community-Centered Development (CCD) dengan tahapan persiapan, sosialisasi, CCD, dan Pendampingan.  Hasil pengabdian masyarakat ini adalah UMKM akan dapat menggunakan pemasaran digital, dimulai dari pembuatan konten media sosial hingga penggunaan iklan media sosial, untuk memasarkan produk mereka. Tim membantu UMKM Poklahsar Waluya membuat akun sosial media seperti instagram, konten yang menarik pelanggan, serta kemasan produk.
The Implementation of the Madrasah Principal's Policies in Enhancing the Competence of Educators and Educational Staff of Islamic Junior High School (Perspective of the Independent Curriculum)
Authors: Dwiyono Yudo , Tannarong Yudhy
Published: June 2024

This research aims to analyze the policies, implementation strategies, and influence of the policies of the Principal of the Islamic Middle School to improve the competence of educators and education staff based on the independent curriculum perspective. This research used a qualitative approach with a case study method. The informants in this research were the school principal, deputy principal for curriculum, educators/teachers, and education staff. The data was collected through in-depth interviews, observations, and documentary studies. The data analysis technique used the Miles and Huberman model with the following stages: (1) Data Collection, (2) Data Reduction, (3) Data Presentation, (4) Conclusion Drawing/Verification. Data validity used triangulation techniques, including source and technical triangulation. The results of the research show that: (1) the policies implemented by the principal of the Islamic Middle School, among others, recruitment of educators and educational staff is always adjusted to their qualifications or abilities, placement of educators and educational staff is based on competency, fostering active and communicative relationships with educators and educational staff, giving awards to educators and educational staff who excel; (2) the strategy of the principal of the Islamic Middle School includes: regular meetings, training, supervision, Subject Teacher Working Groups (MGMP), follow-up studies, and benchmarking; (3) The influence of the policy of the principal of the Islamic Middle School including increased accreditation, increased student achievement, and high public trust. The conclusion of this research is to implement the Islamic Middle School policy to improve the competence of educators and education personnel from the Independent Curriculum perspective.
LEXICAL DENSITY AND READABILITY IN STUDENTS’ THESIS INTRODUCTIONS
Authors: Siregar May Sarah , Hara Nina Tiya , Sinar Tengku Silvana
Published: June 2024

This study investigates lexical density and readability in students’ undergraduate thesis introductions. Documentation technique is used to collect the data while qualitative content analysis was employed as the method to present the data.  The data of this study were content words and sentences taken from the data sources of 20 undergraduate’s thesis introductions. The scores of lexical density and readability were obtained by using Flesch Reading Ease Tool online. The findings show that all introductions exhibit high lexical density, with scores ranging from 51,28% to 68,08%. Whilst, the readability scores range from 10.92 to 17.58, and they are all categorized as being very difficult writings. In summary, the findings demonstrate that all thesis introductions exhibit a high level of complexity in their writing. These introductions showcase the deliberate use of rich and precise content words. Consequently, they pose a significant challenge for comprehension due to their density.
MAKNA SIMBOL ARSITEKTUR RUMAH: PEMERTAHANAN GLOSARIUM KEBUDAYAAN SUKU SASAK
Authors: Sam'un Ahmad , Riadi Selamet , Idham Idham
Published: June 2024

Penelitian ini beranjak dari terus berkembangnya pembauaran perilaku masyarakat suku asli pulau Lombok dengan dunia luar khusunya dibidang arsitektur rumah yang dibangun. Perkembangan arsitektur tersebut merupakan suatu hal yang sangat baik, tetapi di sisi lainnya berbotensi memudarkan nilai filosofis dan makna yang terkandung di dalam model penerapan perilaku pembangunan tempat tinggal oleh masyarakat suku Sasak untuk menjaga keharmonisan antar masyarakat dan alam sekitarnya. Potensi-potensi yang bisa saja terjadi inilah kemudian khendak divalidasi apakah sudah terjadi, sejauhmana atau bagaimana masyarakat setempat mempertahankan makna dalam kebudayaannya sendiri. Makna yang terkandung kemudian dideskripsikan untuk kemudian dapat dimanfaatkan oleh berbagai pihak dan bidang keilmuan dalam rangka mewujudkan harmonisasi dan keindahan perilaku di tengah masyarakat dan alam sekitar. Hasil penelitian ini kemudian dapat juga dikembangkan menjadi sumber belajar kontekstual bagi masyarakat setempat. Penelitian ini mengandung deskripsi makna dalam simbol arsitektur rumah suku Sasak serta mekanisme pemertahanan kebudayaan dan bahasa serta solusi dalam pemertahana bahasa arsitektur budaya masyarakat. Penulis dalam kajian ini menggunakan pendekatan semiotika dan antropologi serta pemertahanan bahasa. Pengumpulan data dilakukan menggunakan metode cakap dengan teknik cakap semuka, serta dikombinasikan dengan teknik rekam dan teknik catat. Berdasarkan pengkajian objek penelitian ditemukan data arah mata angin dan simbol-simbol arsitektur khas suku Sasak yang memiliki makna unik dalam kehidupan masyarakat Sasak, desa Perina, Kecamatan Jonggat, Kabupaten Lombok Tengah, Provinsi Nusa Tenggara barat.
Vatican diplomacy around Trianon from a Hungarian Perspective
Authors:   Pázmány Péter Catholic University, Birher Nándor , Bertalan Péter Tamás ,   Institute of historic science, Budapest
Published: June 2024

The document, titled "Vatican Diplomacy Around Trianon from a Hungarian Perspective," is a scholarly work that examines the geopolitical and ecclesiastical changes in East-Central Europe after the Treaty of Trianon in 1920, with a focus on the role of the Vatican. The book explores the interactions between the Vatican and the reshaped geopolitical landscape, emphasizing the influence of religious and ecclesiastical issues on international relations. The monograph posits that Central Europe is not merely a geographic or economic concept but a "spiritual kingdom" with a shared destiny shaped by interdependence and cultural achievements, despite its history of conflicts. Central Europe's rich intellectual and cultural heritage, deeply influenced by religious convictions and Vatican diplomacy, continues to impact the region's identity and unity. The book suggests that the lessons from the early 20th century, including the Trianon Peace Treaty and subsequent geopolitical shifts, are still relevant in understanding the contemporary political and cultural dynamics of Central Europe. The authors encourage readers to reflect on the past and consider how historical events and decisions have shaped present-day Central Europe. The book aims to provide insights into the region's complex history and its ongoing struggle to find its place in the broader European and global context.
Fanny Brawne and Criticism
Authors: Nersessian Anahid
Published: June 2024

This paper considers the letters and the character of Frances ‘Fanny’ Brawne together as a model for criticism at the present time, as well as a meaningful indication of how criticism was practiced by laypeople in the Romantic period. Focusing on Keats’s great love affair rather than on Keats himself, or on Keats to the exclusion of his interlocuters, I describe the erotic conflict between Brawne and the poet as a form of agonistic engagement between writer and critic–the one tragic in impulse, the other more comic. Toward the end of the essay, I ask how conflict of this kind may be understood, in Stanley Cavell’s terms, not as the ugly underside of love but as one of its most significant expressions, a way of keeping up (in Cavell’s words) ‘the conversation love demands’. Throughout, the essay queries the relation between criticism and love, and how that relation might contest contemporary thinking around affect and pleasure.
Shell Composition Analysis of European Flat Oyster (Ostrea edulis, Linnaeus 1758) From Marmara Sea, Türkiye: Insights Into Chemical Properties
Authors: Kızılkaya Bayram , Yıldız Harun , Vural Pervin
Published: June 2024

The chemical structure of Ostrea edulis (O. edulis) shells was investigated in this work. The study determined zero charge points (PZC) of Ostrea edulis shells. The shell surface charge status is indicated by the PZC value. It was found that the shell PZC value was 8.30. The shells were subjected to Energy Dispersive X-Ray Spectroscopy (EDS) analyses and scanning electron microscope (SEM) pictures. The main structure of calcium carbonate (CaCO3) is made up of carbon, oxygen, and calcium atoms, which were found in the largest quantities based on the EDS data. The structure of CaCO3 was supported by Fourier Transform Infrared Spectroscopy (FT-IR) analysis. As part of the study, X-Ray Diffraction (XRD) investigations were conducted, and it was found that the shell structures are primarily composed of an aragonite and CaCO3 mixture. As is well known, CaCO3, which makes up roughly 94% of the shell, is the primary constituent of bivalves’ shells. This research offers a thorough examination of the chemical makeup of O. edulis shells. This study is thought to serve as the foundation for further research on the biological and chemical characteristics of marine species.
Tinjauan Yuridis Terhadap Akibat Tidak Melaksanakan Putusan Pengadilan Agama Terkait Pemberian Nafkah Pasca Cerai
Authors: Rohmawati Fauziyah
Published: June 2024

This research was motivated by the fact that quite a few parents neglected their responsibilities and obligations to their children after divorce, to the point that their children became neglected. This study will examine the court decision regarding post-divorce maintenance in case no. 2569/Pdt.G/2022/PA.Gs, a judicial review of the consequences of not implementing the court decision regarding post-divorce maintenance in case no. 2569/Pdt.G/2022/PA.Gs. as well as legal measures that can be taken by the wife and children in case No. 2569/Pdt.G/2022/PA.Gs. The methodology used in writing this scientific work is library research. The type of research is normative using a case approach. The research results show that Decision No. 2569/Pdt.G/2022/PA. Gs The implementation of the contents of the decision was not carried out properly. The consequences for not paying child support include child neglect (articles 76 and 77 of Law No. 35 of 2014) which carries a maximum penalty of imprisonment for 5 (five) years and/or a maximum fine of IDR 100,000,000. 00 (one hundred million rupiah). So the legal action that can be taken by the ex-wife is to submit a forced effort to the court against the ex-husband to provide maintenance, which is called a request for execution of the decision. The legal instrument used in the decision is Article 195-197 HIR, providing legitimacy to parties who feel their rights in the household have been violated to demand justice
RANCANG BANGUN SISTEM INFORMASI MONITORING BIMBINGAN TUGAS AKHIR BERBASIS WEB MENGGUNAKAN FRAMEWORK REACTJS
Authors: Setiawan La Ode Muhammad Ilham , Gunawan  , Tenriawaru Andi
Published: June 2024

Proses bimbingan skripsi menjadi bagian penting dari proses pengerjaan tugas akhir yang dilakukan oleh mahasiswa. Bimbingan dilakukan dengan melibatkan dosen pembimbing dengan tujuan memberi tahu capaian yang telah dicapai dalam penulisan tugas akhir. Berdasarkan hasil observasi yang dilakukan, proses bimbingan tugas akhir yang ada di Program Studi Ilmu Komputer Universitas Halu Oleo masih dilaksanakan secara tatap muka sehingga sering terjadi komunikasi antara mahasiswa dan dosen pembimbing. Untuk mengefisienkan proses monitoring bimbingan tugas akhir mahasiswa Program Studi Ilmu Komputer. ReactJs merupakan library front-end yang dikembangkan oleh Meta. Keunggulan yang ditawarkan oleh reactJs diantaranya memberikan kecepatan, kemudahan dan skalabilitas yang luas. Tujuan dari penelitian ini adalah menghasilkan sebuah sistem informasi layanan monitoring bimbingan tugas akhir pada Program Studi Ilmu Komputer mengunakan framework ReactJS. Pemilihan reactJs dalam penelitian ini dikarenakan keunggulan serta kelebihan yang diberikannya. ReactJs dapat mengembangkan single page application dengan kecepatan, kemudahan serta skalabilitas yang luas. Karena kelebihan yang ditawarkan inilah framework reactJs mampu membantu mengembangkan sistem monitoring tugas akhir lebih efisien dan cepat. Berdasarkan hasil pengujian fungsional yang dilakukan menggunakan blackbox telah menunjukan hasil yang baik, begitu juga berdasarkan hasil pengujian menggunakan system usability scale yang dilakukan pada dosen dan mahasiswa telah menunjukan skor 87 dari 100 dengan kategori excellent serta skala A untuk pada pengujian SUS dosen serta skor 73 dari 100 dengan kategori Good  serta grade scale yaitu B pada pengujian SUS mahasiswa. Dengan tingkat penerimaan untuk dosen dan mahasiswa berada pada skala accpetable atau dapat diterima.
EVALUASI ALAT PENDUKUNG PADA MANAJEMEN PROYEK PERANGKAT LUNAK
Authors: Hidayatullah Muhammad Andrean , Mangkunegara Putra Ki Ageng Nasrokh , Yaqin Muhammad 'Ainul , Hidayatullah Muhammad Andrean
Published: June 2024

Manajemen proyek perangkat lunak merupakan aspek krusial dalam pengembangan perangkat lunak yang efektif dan efisien. Salah satu tantangan utama dalam manajemen proyek perangkat lunak adalah pemilihan alat pendukung yang tepat untuk memastikan keberhasilan proyek. Penelitian ini mengevaluasi berbagai alat pendukung manajemen proyek perangkat lunak dengan tujuan mengidentifikasi alat yang paling efektif berdasarkan kriteria tertentu seperti kemudahan penggunaan, fitur yang disediakan, integrasi dengan alat lain, dan dukungan teknis. Penelitian ini mengusulkan solusi berupa penggunaan metodologi evaluasi berbasis kriteria yang telah ditentukan, dan kemudian menguji beberapa alat populer seperti Jira, Trello, Asana, Docker dan Microsoft Project. Hasil eksperimen menunjukkan bahwa Jira telah terbukti menjadi pilihan terbaik dengan skor tertinggi secara keseluruhan. Namun, Asana dan Microsoft Project juga menunjukkan performa yang baik dalam beberapa kategori, sementara Docker dan Trello cenderung memiliki skor lebih rendah. Tafsiran hasil ini menunjukkan Pemilihan alat manajemen proyek harus didasarkan pada analisis yang cermat terhadap kebutuhan spesifik proyek dan kriteria yang paling penting bagi tim yang terlibat. Oleh karena itu, keberhasilan implementasi alat bantu bergantung pada pemahaman yang mendalam tentang proses pengembangan perangkat lunak, dinamika tim, dan keterampilan manajemen yang kuat.
Differential Sensitivity Bounds for Dynamic Control
Authors: O'Neil Sean , Schirmer Sophie
Published: June 2024

This dataset contains the code and data for investigation of the differential sensitivity of the gate infidelity to parametric (structured) uncertainty. The provided controllers are piecewise constant, dynamic controls optimized to maximize gate fidelity across nine problems of varying system complexity and control paradigms. The analysis code computes bounds on the differential sensitivity and statistically analyses the relationship between the infidelity and sensitivity bounds. The analysis code also provides plots to aid in visualization of the statical data.
Between Rootedness and Alienation: An Ecological Reading of Hala Alyan's Salt Houses
Authors: AlRamahi Samia , Al-Shetawi Mahmoud F.
Published: June 2024

The overlapping of the theoretical framework of Ecocriticism and Postcolonialism highlights the interdisciplinarity between the environment and literature and the possibility of using elements of either of them to reflect and overwhelm the other. This overlap becomes a significant case study in depicting Anglophone writers' yearning in the diaspora or at home while writing about their homelands, reflecting their longing to belong while suffering from alienation and up-rootedness. It is worth mentioning that most widely different literary works that blend ecocriticism with postcolonialism concentrate on the idea of devastation or mishandling of nature because of colonialism and other causes. However, this research will attempt to deviate from these critical readings in handling this interdisciplinarity, as it will examine the reflections of the environmental elements with postcolonial concepts of alienation and rootedness on people's feelings and emotions in the diaspora by offering a critical reading for Hala Alyan's Salt Houses (2017). The novel reveals the role of natural environmental elements like plants, gardens, and trees in reflecting the characters' senses of rootedness and belonging, while water, seas, and oceans describe their senses of alienation and estrangement. Salt Houses draws on how the dispossessed Palestinians' cultivation and regeneration of their gardens and plants drive their struggle, resistance, and existence against the Israeli occupation.
Care Burden and Compassion in Caregivers of Stroke Survivors
Authors: Erdem Yüzbaşıoğlu Hatice , Kars Fertelli Tülay
Published: June 2024

Objective: This study was conducted to evaluate the factors related to care burden and compassion levels of caregivers of family members who had a stroke and the relationship between the two variables.
Methods: The study was designed as a descriptive, cross-sectional, and correlational one. The sample included 280 stroke survivors and family caregivers. The data were collected using a Family Caregiver Information Form, the Stroke Survivors Information Form, the Zarit Burden Interview (ZBI), the Compassion Scale (CS), and the Modified Barthel Index (MBI).
Results: In the study, it was determined that the compassion scores (3.71±0.85), and caregiving burden scores (34.30±17.42) of the family caregivers of stroke survivors were moderate. There was a negative, strong and significant relationship between caregiving burden and compassion (p
The Relationship between Menopause Perception, Body Mass Index, and Waist-Hip Ratio with Menopausal Symptoms in Turkish Women
Authors: Uyanık Abdurrahim , Yıldız Hatice
Published: June 2024

Objective: The study aimed to investigate the relationship between menopause perceptions, feelings felt, body mass index, and waist-hip ratio with menopausal symptoms in Turkish climacteric women.
Methods: This descriptive and correlational study was conducted in a Family Health Center. The research sample consisted of 220 women in the climacteric period. Data were collected with the survey form and The Menopause Rating Scale (MRS). Body Mass Index (BMI) and Waist Hip Ratio (WHR) were measured and calculated by the researchers.
Results: The women who perceive menopause as a “natural, normal process” had lower somatic, psychological, and general menopausal symptoms, and those who defined it as “the end of sexuality” had higher genitourinary symptoms (p< .01). Women who were adversely affected or felt negative emotions about menopause reported that they experienced all menopausal symptoms more severely (p< .001). Obese women experienced particularly higher levels of somatic and general menopausal symptoms (p< .05-p< .01), while women with WHR< 0.72 experienced a higher level of genitourinary symptoms (p< .05).
Conclusion: The results showed that menopause perception, feelings felt, obesity, and WHR have an impact on menopausal symptoms and levels.
Computer-aided design and implementation of efficient biosynthetic pathways to produce high added-value products derived from tyrosine in Escherichia coli
Authors: Ferreira Sofia , Balola Alexandra , Sveshnikova Anastasia , Hatzimanikatis Vassily , Vilaça Paulo , Maia Paulo , Carreira Rafael , Stoney Ruth , Carbonell Pablo , Souza Caio Silva , Correia João , Lousa Diana , Soares Cláudio M. , Rocha Isabel
Published: June 2024

Developing efficient bioprocesses requires selecting the best biosynthetic pathways, which can be challenging and time-consuming due to the vast amount of data available in databases and literature. The extension of the shikimate pathway for the biosynthesis of commercially attractive molecules often involves promiscuous enzymes or lacks well-established routes. To address these challenges, we developed a computational workflow integrating enumeration/retrosynthesis algorithms, a toolbox for pathway analysis, enzyme selection tools, and a gene discovery pipeline, supported by manual curation and literature review. Our focus has been on implementing biosynthetic pathways for tyrosine-derived compounds, specifically L-3,4-dihydroxyphenylalanine (L-DOPA) and dopamine, with significant applications in health and nutrition. We selected one pathway to produce L-DOPA and two different pathways for dopamine–one already described in the literature and a novel pathway. Our goal was either to identify the most suitable gene candidates for expression in Escherichia coli for the known pathways or to discover innovative pathways. Although not all implemented pathways resulted in the accumulation of target compounds, in our shake-flask experiments we achieved a maximum L-DOPA titer of 0.71 g/L and dopamine titers of 0.29 and 0.21 g/L for known and novel pathways, respectively. In the case of L-DOPA, we utilized, for the first time, a mutant version of tyrosinase from Ralstonia solanacearum. Production of dopamine via the known biosynthesis route was accomplished by coupling the L-DOPA pathway with the expression of DOPA decarboxylase from Pseudomonas putida, resulting in a unique biosynthetic pathway never reported in literature before. In the context of the novel pathway, dopamine was produced using tyramine as the intermediate compound. To achieve this, tyrosine was initially converted into tyramine by expressing TDC from Levilactobacillus brevis, which, in turn, was converted into dopamine through the action of the enzyme encoded by ppoMP from Mucuna pruriens. This marks the first time that an alternative biosynthetic pathway for dopamine has been validated in microbes. These findings underscore the effectiveness of our computational workflow in facilitating pathway enumeration and selection, offering the potential to uncover novel biosynthetic routes, thus paving the way for other target compounds of biotechnological interest.
Clinical characteristics, viral dynamics, and antibody response of monkeypox virus infections among men with and without HIV infection in Guangzhou, China
Authors: Yang Huiqin , Xie Xiaoqing , Zeng Mou , Cao Yinghui , Fan Qinghong , Jiang Mengling , Lei Chunliang , Wang Jian , Li Feng , Tang Xiaoping , Yu Haisheng , Li Linghua
Published: June 2024

Background

Monkeypox virus (MPXV) is spreading globally and nearly half of the infected people were human immunodeficiency virus (HIV) positive. Therefore, an in-depth understanding of the effects of HIV infection on the outcomes of MPXV infection is urgently needed. This study aimed to explore the clinical features, viral dynamics, and antibody response to MPXV infections in men who had sex with men (MSM) with and without HIV co-infection.

Design or methods

MPXV-infected patients diagnosed by PCR were recruited in this study and were divided into MPXV and MPXV + HIV groups based on whether they were co-infected with HIV. Clinical data and samples were collected during of the hospital stay and follow up interviews. The symptoms and signs, laboratory examinations, viral shedding in various body fluids or swabs, antibody dynamics were tracked and compared between the two groups.

Results

A total of 41 MPXV patients were recruited through June 2023 to September 2023 in Guangzhou. The MPXV group and MPXV + HIV group comprised 20 and 21 MSM, respectively. Patients in the two groups exhibited similar clinical characteristics except for pruritus and eschar, both were significantly fewer in MPXV + HIV group than in MPXV only group. Among the 355 clinical samples collected, MPXV DNA was detected in 100% of scabs, 97.4% of skin swabs, and 92.3% of exudate swabs from lesions, while the positive rate was 87.5% from oropharyngeal swabs, 59% from saliva, 51.3% from anal swabs, 50% from feces, 30.6% from urine samples, 37.5% of semen, and 28.2% from sera. Dynamics analysis revealed that viral DNA was undetectable in most patients 20 days after symptom onset. IgM and IgG antibodies to MPXV were detected in all patients with 3–5 days earlier in the MPXV group than in the MPXV + HIV group.

Conclusion

This cohort analysis based on a large outbreak among MSM in Guangzhou indicated no obvious differences in clinical symptoms, viral DNA data, but antibody responses were 3–5 days later in mpox patients with HIV infection.
Effects of high-intensity intermittent exercise on glucose and lipid metabolism in type 2 diabetes patients: a systematic review and meta-analysis
Authors: Feng Jingwen , Zhang Qiuhua , Chen Baoyi , Chen Jinping , Wang Wenjun , Hu Yuhang , Yu Jiabin , Huang Huiming
Published: June 2024

Objective

To evaluate the effects of high-intensity interval training (HIIT) on glycolipid metabolism among type 2 diabetes patients.

Methods

HIIT is consistent with an exercise program (65%-90%VO2max or 75%-95% HRmax; exercise cycle≥2 weeks; frequency ≥ 2 times/week). A meta-analysis was conducted utilizing the random effects model to synthesize the data.

Results

A total of 22 RCT studies with 1034 diabetic patients were included. Compared to moderate-intensity aerobic exercise or conventional controls, HIIT yields noteworthy effects on FBG (MD: -0.55; 95% CI: -0.85- -0.25, Hedges’ g =0.98), 2h-PG (MD: -0.36; 95% CI: -0.57- -0.14, Hedges’ g =1.05), FINS (MD: -0.41; 95% CI: -0.79- -0.03, Hedges’ g =1.07), HbA1c (MD: -0.60; 95% CI: -0.84- -0.36, Hedges’ g =2.69), TC (MD: -0.58; 95% CI: -0.80- -0.36, Hedges’ g =2.36), TG (MD: -0.50; 95% CI: -0.86- -0.14, Hedges’ g =1.50), HDL (MD: 0.62; 95% CI: 0.29–0.95, Hedges’ g =1.19) and LDL (MD: -0.31; 95% CI: -0.56- -0.08, Hedges’ g =0.91), all of the above p<0.01.

Conclusions

HIIT has been shown to improve glucose and lipid metabolism in patients with type 2 diabetes, especially in HbA1c, TC, TG, and HDL. For patients between the ages of 40 and 60 with less than 5 years of disease, exercise programs of moderate to longer duration or moderate to high intensity will produce more favorable results.
The m6A-ncRNAs axis in diabetes complications: novel mechanism and therapeutic potential
Authors: Yu Siming , Li Chunsheng , Lu Xinxin , Han Zehui , Li Yue , Yuan Xingxing , Guo Dandan
Published: June 2024

Diabetes, a multifaceted metabolic disorder, poses a significant global health burden with its increasing prevalence and associated complications, such as diabetic nephropathy, diabetic retinopathy, diabetic cardiomyopathy, and diabetic angiopathy. Recent studies have highlighted the intricate interplay between N6-methyladenosine (m6A) and non-coding RNAs (ncRNAs) in key pathways implicated in these diabetes complications, like cell apoptosis, oxidative stress, and inflammation. Thus, understanding the mechanistic insights into how m6A dysregulation impacts the expression and function of ncRNAs opens new avenues for therapeutic interventions targeting the m6A-ncRNAs axis in diabetes complications. This review explores the regulatory roles of m6A modifications and ncRNAs, and stresses the role of the m6A-ncRNA axis in diabetes complications, providing a therapeutic potential for these diseases.
Energy balancing strategy for the multi-storage islanded DC microgrid based on hierarchical cooperative control
Authors: Xie Chen , Wei Maohua , Luo Dongtao , Yang Ling
Published: June 2024

To simultaneously solve the problems of the state-of-charge (SOC) equalization and accurate current distribution among distributed energy storage units (DESUs) with different capacities in isolated DC microgrids, a multi-storage DC microgrid energy equalization strategy based on the hierarchical cooperative control is proposed. In the primary control layer, the link between the droop coefficient and SOC is established through a logarithmic function, and the droop coefficient is adaptively adjusted according to the SOC value in order to achieve fast SOC equalization. In the secondary control layer, by designing a coordinated state factor, only one PI controller is required to eliminate the influence of the line impedance on the accurate distribution of the output current and the DC bus voltage drop. In the communication layer, local nodes only need to communicate with neighboring nodes without the need for a central controller, and the dynamic consistency algorithm is used to obtain the average value information about the energy storage system (ESS). Finally, the feasibility and effectiveness of the proposed control strategy are verified by experimental analysis on the DC microgrid hardware-in-the-loop experimental platform.
Spatial-temporal changes of landscape ecological risk in the Liuchong river basin from the perspective of production-life-ecological space
Authors: Ren Jintong , Dong Yanqin , He Panxing , Lu Hanyu
Published: June 2024

Promoting the construction of ecological civilization and sustainable development in karst mountainous areas by analyzing the spatial and temporal changes of landscape ecological risks is critical in karst mountainous watersheds. In this study, the land use transfer matrix, landscape ecological risk evaluation model, ecological contribution rate of land use change, and spatial autocorrelation analysis were combined to quantitatively analyze the land use and landscape ecological risk of a typical karst watershed, Liuchong River Basin, over the past 20 years. The results revealed that: 1) From 2000 to 2020, the functional classification of land use in the Liuchong River Basin was dominated by the woodland ecological space, and the most significant shifting characteristics were the increase in the area of watershed ecological space and industrial production space and the decrease in woodland ecological space, with shifts in the middle reaches of the Liuchong River being the most drastic; 2) Generally, the change of the regional landscape pattern was related to the transformation of the land use function type of “production-life-ecological space,” and the spatial aggregation of ecological risk level showed a gradual weakening trend. 3) The conversion of the watershed ecological space to the grassland ecological and agricultural production spaces, the conversion of urban living space to the agricultural production space, and the conversion of the rural living space to the agricultural production space were the dominant factors affecting ecological improvement, whereas the conversion of the woodland ecological space to the grassland ecological space, the woodland ecological space to the agricultural production space, and the grassland ecological space to the agricultural production space contributed to ecological degradation. The study findings can be used as a reference for the coordinated development of “production-life-ecological space” in karst watersheds and provide a scientific basis for ecological environmental protection and sustainable utilization.
CXCR4 has a dual role in improving the efficacy of BCMA-redirected CAR-NK cells in multiple myeloma
Authors: Moles Michael W. , Erdlei Henry , Menzel Lutz , Massaro Marialucia , Fiori Agnese , Bunse Mario , Schrimpf Moritz , Gerlach Kerstin , Gudipati Venugopal , Reiser John , Mathavan Ketan , Goodrich Jodie P. , Huppa Johannes B. , Krönke Jan , Valamehr Bahram , Höpken Uta E. , Rehm Armin
Published: June 2024

Multiple myeloma (MM) is a plasma cell disease with a preferential bone marrow (BM) tropism. Enforced expression of tissue-specific chemokine receptors has been shown to successfully guide adoptively-transferred CAR NK cells towards the malignant milieu in solid cancers, but also to BM-resident AML and MM. For redirection towards BM-associated chemokine CXCL12, we armored BCMA CAR-NK-92 as well as primary NK cells with ectopic expression of either wildtype CXCR4 or a gain-of-function mutant CXCR4R334X. Our data showed that BCMA CAR-NK-92 and -primary NK cells equipped with CXCR4 gained an improved ability to migrate towards CXCL12 in vitro. Beyond its classical role coordinating chemotaxis, CXCR4 has been shown to participate in T cell co-stimulation, which prompted us to examine the functionality of CXCR4-cotransduced BCMA-CAR NK cells. Ectopic CXCR4 expression enhanced the cytotoxic capacity of BCMA CAR-NK cells, as evidenced by the ability to eliminate BCMA-expressing target cell lines and primary MM cells in vitro and through accelerated cytolytic granule release. We show that CXCR4 co-modification prolonged BCMA CAR surface deposition, augmented ZAP-70 recruitment following CAR-engagement, and accelerated distal signal transduction kinetics. BCMA CAR sensitivity towards antigen was enhanced by virtue of an enhanced ZAP-70 recruitment to the immunological synapse, revealing an increased propensity of CARs to become triggered upon CXCR4 overexpression. Unexpectedly, co-stimulation via CXCR4 occurred in the absence of CXCL12 ligand-stimulation. Collectively, our findings imply that co-modification of CAR-NK cells with tissue-relevant chemokine receptors affect adoptive NK cell therapy beyond improved trafficking and retention within tumor sites.
Rational approach to the prescription of anti-rheumatic drugs in rheumatoid arthritis: a product leaflet-based strategy in Italy
Authors: Perricone Carlo , Castellucci Andrea , Cafaro Giacomo , Calvacchi Santina , Bruno Lorenza , Dal Pozzolo Roberto , Tromby Francesco , Colangelo Anna , Gerli Roberto , Bartoloni Elena
Published: June 2024

The treatment of patients with rheumatoid arthritis (RA) has dramatically changed in the past 30 years. Currently, numerous conventional, biologic, and targeted synthetic DMARDs have been licensed and used following recommendations provided by international and national scientific societies. However, the availability of biosimilars and the increasing necessity of savings impacted on the local/national prescription of these drugs. The information provided by data sheet of every single drug is a decisive factor on the choice of a certain treatment merged with the patient’s profile. Thus, our purpose was to construct a rational algorithm for the treatment strategy in RA according to costs and the product leaflet of the biologic and targeted-synthetic DMARDs currently licensed in Italy. We used the most recent available recommendations and then we performed a review of the literature considering all the factors that are known to influence drug safety/effectiveness. All these factors were considered in the context of the data sheets of currently available originators and biosimilars.
Effects of flows on transparent exopolymer particles released from branching Acropora coral colonies
Authors: Wu Heng , Yamada Yosuke , Chuang Po-Shun , Ishikawa Kota , Mitarai Satoshi
Published: June 2024

Transparent exopolymer particles (TEP), a major component of coral mucus, are responsible for particle aggregation. These particles contribute substantially to the carbon cycle in coral reefs, and serve as an energy source for bacteria and other microorganisms. Water flows and induced turbulent mixing control material exchange between the coral canopy and the surrounding water, which is critical for coral health. However, how these factors affect TEP release by coral colonies has yet to be evaluated. Using a recirculating flume, we assessed TEP release by branching Acropora coral colonies and associated bacterial growth in the water column under different unidirectional flows. Changes in TEP and bacterial concentrations after 24-h incubation were quantified for flow speeds of 0, 5, 10, and 30 cm/s. Particle image velocimetry (PIV) measurements provided an estimate of turbulent mixing efficiency above the coral canopy. TEP and bacterial concentrations in the water column increased after 24 h of incubation. The increase in TEP and bacterial concentrations were 6.2–9.3 times and 3.4–5.1 times higher in the absence of flows, respectively, than mean values under water flows. Although mixing efficiency increased linearly with mean flow speeds, TEP release and bacterial growth differed only marginally at flows ranging from 5–30 cm/s. Detailed flow measurements combined with evaluation of TEP release suggest that the complex geometry of corals facilitates efficient material exchange at a range of flow speeds, and highlight the importance of considering these factors when estimating coral reef biogeochemistry.
Bioinformatics and system biology approach to identify potential common pathogenesis for COVID-19 infection and sarcopenia
Authors: Zhong Jun , Yuan Hui , Yang Jinghong , Du Yimin , Li Zheng , Liu Xu , Yang Haibo , Wang Zhaojun , Wang Zi , Jiang Lujun , Ren Zhiqiang , Li Hongliang , Li Zhong , Liu Yanshi
Published: June 2024

Sarcopenia is a condition characterized by age-related loss of muscle mass and strength. Increasing evidence suggests that patients with sarcopenia have higher rates of coronavirus 2019 (COVID-19) infection and poorer post-infection outcomes. However, the exact mechanism and connections between the two is unknown. In this study, we used high-throughput data from the GEO database for sarcopenia (GSE111016) and COVID-19 (GSE171110) to identify common differentially expressed genes (DEGs). We conducted GO and KEGG pathway analyses, as well as PPI network analysis on these DEGs. Using seven algorithms from the Cytoscape plug-in cytoHubba, we identified 15 common hub genes. Further analyses included enrichment, PPI interaction, TF-gene and miRNA-gene regulatory networks, gene-disease associations, and drug prediction. Additionally, we evaluated immune cell infiltration with CIBERSORT and assessed the diagnostic accuracy of hub genes for sarcopenia and COVID-19 using ROC curves. In total, we identified 66 DEGs (34 up-regulated and 32 down-regulated) and 15 hub genes associated with sarcopenia and COVID-19. GO and KEGG analyses revealed functions and pathways between the two diseases. TF-genes and TF-miRNA regulatory network suggest that FOXOC1 and hsa-mir-155-5p may be identified as key regulators, while gene-disease analysis showed strong correlations with hub genes in schizophrenia and bipolar disorder. Immune infiltration showed a correlation between the degree of immune infiltration and the level of infiltration of different immune cell subpopulations of hub genes in different datasets. The ROC curves for ALDH1L2 and KLF5 genes demonstrated their potential as diagnostic markers for both sarcopenia and COVID-19. This study suggests that sarcopenia and COVID-19 may share pathogenic pathways, and these pathways and hub genes offer new targets and strategies for early diagnosis, effective treatment, and tailored therapies for sarcopenia patients with COVID-19.
Serum parameters of inflammatory markers as prognostic biomarkers with maternal-neonatal outcome in patients with GDM
Authors: Xiu Xiaoyan , Lin Yingying , Chen Zhiwei , Lin Lin , Zu Yizheng , Yan Jianying
Published: June 2024

Objective

Gestational diabetes mellitus (GDM) is one of the most common complications of pregnancy, which is increasing annually. GDM can cause serious harm to both the mother and the offspring. However, the clinical indicators that predict pregnancy outcomes with GDM remain limited.

Methods

This study included 3,229 pregnancies. Inflammatory markers were defective in the mother’s peripheral blood. Also, the Chi-square test, logistic regression analyses and Spearman rank correlation coefficient were performed to evaluate inflammatory markers with pregnancy outcomes. The association between inflammatory markers and pregnancy outcomes was analyzed. The optimal cut-off values of inflammatory markers were calculated.

Results

Finally, 3,229 women were included. 1852 (57.36%) participants suffered good pregnancy outcomes. This study revealed that the maternal age, the baseline BMI (kg/m2), the times of parity, and the level of lymphocyte, SII and SIRI significantly increased in poor pregnancy outcomes groups. Additionally, inflammatory markers, such as white blood cells (WBC), neutrophils, monocytes, platelet counts, lymphocytes, systemic immune-inflammation index (SII) and systemic inflammation response index (SIRI) were related to pregnancy outcomes. Furthermore, the results revealed that the SII level had the highest odd rates (OR) [OR = 6.957; 95% CI (5.715–8.468)], followed by SIRI level [OR = 2.948; 95% CI (2.382–3.649)], the WBC counts [OR = 1.930; 95% CI (0.901–2.960)], the lymphocyte counts [OR = 1.668; 95% CI (1.412–1.970)], and baseline BMI [OR = 1.050; 95% (1.021–1.080)].

Conclusion

This study presented that the baseline SII and SIRI levels can be valuable biochemical markers to predict the pregnancy outcome with GDM with non-invasive procedures. They can help identify high-risk pregnant women with GDM early, provide a personalized intervention in time, and enhance perinatal surveillance.
Differential distribution of plasma apoA-I and apoB levels and clinical significance of apoB/apoA-I ratio in ischemic stroke subtypes
Authors: Van Tuyen Nguyen , Hoang Ngoc Nguyen , Quoc Hoan Phan , Thi Yen Nguyen , Hoan Nghiem Xuan , Cam Thach Nguyen
Published: June 2024

Background and purpose

Ischemic stroke (IS) is classified into clinical subtypes and likely influenced by various lipid components. Nevertheless, the roles of apolipoprotein A-I (apoA-I), apolipoprotein B (apoB), and apoB/apoA-I ratio in different IS subtypes remain underexplored. This study aimed to investigate the differential distribution of plasma apoA-I and apoB levels among IS subtypes and to evaluate the predictive value of the apoB/apoA-I ratio in assessing IS subtypes and disease severity.

Methods

In this study, 406 IS patients were categorized into three IS-subtypes based on clinical manifestations and imaging assessment, including intracranial atherosclerosis-related IS patients (ICAS, n = 193), extracranial atherosclerosis-related IS patients (ECAS, n = 111), and small artery occlusion-related IS patients (SAO, n = 102). Plasma apoA-I and apoB levels were measured upon hospital admission. Random forest (RF) models were performed to assess predictive values of these apolipoproteins apoB, apoA-I and their ratio in assessing IS subtype stratification and disease severity.

Results

Serum apoA-I levels were significantly lower in ICAS compared to ECAS and SAO patients (p < 0.0001), while apoB levels were higher in ICAS patients (p < 0.0001). The apoB/apoA-I ratio was significantly higher in ICAS compared to ECAS and SAO patients (p < 0.0001). Correlation analyses found a significant correlation between the apoB/apoA-I ratio and conventional lipid components. Additionally, RF models and plots of variable importance and distribution of minimal depth revealed that the apoB/apoA-I ratio played the most influential predictor in predicting IS subtypes and stenosis severity.

Conclusion

Our study shows the differential distribution of apoA-I and apoB IS subtypes and reveals the significance of the apoB/apoA-I ratio in assessing IS subtypes and arterial stenosis severity. Further studies are warranted to validate these findings and enhance their clinical applicability.