bluuebunny/crossref_metadata_2025 huggingface dataset:
Anticancer effects of OSW-1 on glioma cells via regulation of the PI3K/AKT signal pathway: A network pharmacology approach and experimental validation in vitro and in vivo
Authors: Zhan Zhixin , Liu Ziqiang , Zhang Chaochao , Gao Haijun , Lai Jiacheng , Chen Yong , Huang Haiyan
Published: September 2022

Background: Gliomas are the most common primary intracranial malignant tumors with poor prognosis, despite the remarkable advances in medical technology that have been made. OSW-1, isolated from Ornithogalum saundersiae, possesses anticancer activity against various malignant cancer cells. However, the effects of OSW-1 on gliomas and its potential mechanisms remain unclear.

Methods: Network pharmacology was employed for predicting potential key targets and mechanisms of the anticancer effects of OSW-1 on glioma. Experiments, including the Cell Counting Kit-8, colony formation, and flow cytometry, were performed to investigate how OSW-1 affects the biological behavior of glioma cells in vitro. Western blotting was used to detect changes in related proteins, such as those involved in the cell cycle, apoptosis, and signaling pathways. The nude mouse xenograft model was used to detect the effect of OSW-1 on inhibiting the proliferation of glioma cells in vivo.

Results: An “OSW-1-Targets-Glioma” intersection network consisting of 151 intersecting genes was acquired to construct a “Protein–Protein Interaction network” and predict the top 10 core targets. According to the Kyoto Encyclopedia of Genes and Genomes pathway analysis, the PI3K/AKT signaling pathway was the top 3-ranked pathway, with 38 enriched intersecting genes. The glioma T98G and LN18 cell lines were used to verify the predictions. OSW-1 significantly inhibited the viability and proliferation of glioma cells in a dose- and time-dependent manner. Flow cytometry showed that OSW-1 arrested the cell cycle at the G2/M phase, and the apoptotic ratio of glioma cells increased significantly with increasing concentrations. Western blotting revealed that the expression levels of p-PI3K and p-AKT1 in glioma cells treated with OSW-1 were significantly lower than those in the controls; however, 740Y-P, a PI3K activator, significantly reversed the inactivation of the PI3K/AKT signaling pathway caused by OSW-1. Furthermore, the mouse xenograft model confirmed the suppressive effect of OSW-1 on tumor growth in vivo.

Conclusion: OSW-1 is a promising anti-glioma chemotherapeutic drug owing to its anticancer effects via downregulation of the PI3K/AKT signaling pathway. However, OSW-1 still has a long way to go to become a real anti-glioma drug.
Pharmacological properties, molecular mechanisms and therapeutic potential of ginsenoside Rg3 as an antioxidant and anti-inflammatory agent
Authors: Wang Jing , Zeng Li , Zhang Ying , Qi Wenxiu , Wang Ziyuan , Tian Lin , Zhao Daqing , Wu Qibiao , Li Xiangyan , Wang Tan
Published: September 2022

Inflammation and oxidative stress lead to various acute or chronic diseases, including pneumonia, liver and kidney injury, cardiovascular and cerebrovascular diseases, metabolic diseases, and cancer. Ginseng is a well-known and widely used ethnic medicine in Asian countries, and ginsenoside Rg3 is a saponin isolated from Panax ginseng C. A. Meyer, Panax notoginseng, or Panax quinquefolius L. This compound has a wide range of pharmacological properties, including antioxidant and anti-inflammatory activities, which have been evaluated in disease models of inflammation and oxidative stress. Rg3 can attenuate lung inflammation, prevent liver and kidney function damage, mitigate neuroinflammation, prevent cerebral and myocardial ischemia–reperfusion injury, and improve hypertension and diabetes symptoms. The multitarget, multipathway mechanisms of action of Rg3 have been gradually deciphered. This review summarizes the existing knowledge on the anti-inflammatory and antioxidant effects and underlying molecular mechanisms of ginsenoside Rg3, suggesting that ginsenoside Rg3 may be a promising candidate drug for the treatment of diseases with inflammatory and oxidative stress conditions.
Study on strength characteristics of soft and hard rock masses based on thermal-mechanical coupling
Authors: Qiu Liewang , Xie Liangfu , Qin Yongjun , Liu Xuejun , Yu Guangming
Published: September 2022

For deep resource exploitation and engineering construction, the mechanical properties of soft and hard interbedded rock masses are important factors impacting engineering stability. Simultaneously, the influence of temperature on the strength of deep rock masses poses a significant obstacle to the exploitation of deep resources and the utilization of underground space. In this paper, the particle flow code (PFC2D) is utilized to establish the thermal-mechanical coupling numerical model of soft and hard interbedded rock masses, and then the uniaxial compression response of soft and hard interbedded rock masses following thermal damage is studied. The displacement and contact force produced by applying temperature, as well as the failure strength, strain, and crack development of the specimen after uniaxial compression is analyzed. The findings reveal that: 1) The peak displacement caused by applied temperature increases first and subsequently decreases with the increase of soft rock thickness ratio (Hs/H), whereas the peak displacement increases linearly with increasing temperature. The peak contact force varies in two stages with the increase of the soft rock thickness ratio (Hs/H), and with the same trends. 2) As the soft rock thickness ratio increases (Hs/H), the number of cracks decreases steadily. When the soft rock thickness ratio Hs/H < 0.5, the relationship curve between vertical strain and crack changes in two stages: the stage of crack development along with the stage of vertical strain gradually increases with crack development. When the thickness ratio of soft rock Hs/H > 0.5, the relationship curve changes in three stages: crack development stage, vertical strain increase stage, and vertical strain increase stage with crack development. 3) When the soft rock thickness ratio Hs/H < 0.5, the failure strength gradually decreases as soft rock thickness increases at T = 100°C, 200°C. The failure strength gradually increases as the soft rock thickness increases in general at T = 400°C. Soft rock thickness ratio Hs/H > 0.5, the failure strength increases with the increase of soft rock thickness at T = 300°C, 400°C. At T = 100°C, 200°C, the tendency of the failure strength changes less.
Functional recovery after accidental deep hypothermic cardiac arrest: Comparison of different cardiopulmonary bypass rewarming strategies
Authors: Filseth Ole Magnus , Kondratiev Timofei , Sieck Gary C. , Tveita Torkjel
Published: September 2022

Introduction:Using a porcine model of accidental immersion hypothermia and hypothermic cardiac arrest (HCA), the aim of the present study was to compare effects of different rewarming strategies on CPB on need for vascular fluid supply, level of cardiac restitution, and cerebral metabolism and pressures.

Materials and Methods:Totally sixteen healthy, anesthetized castrated male pigs were immersion cooled to 20°C to induce HCA, maintained for 75 min and then randomized into two groups: 1) animals receiving CPB rewarming to 30°C followed by immersion rewarming to 36°C (CPB30,n= 8), or 2) animals receiving CPB rewarming to 36°C (CPB36,n= 8). Measurements of cerebral metabolism were collected using a microdialysis catheter. After rewarming to 36°C, surviving animals in both groups were further warmed by immersion to 38°C and observed for 2 h.

Results:Survival rate at 2 h after rewarming was 5 out of 8 animals in the CPB30group, and 8 out of 8 in the CPB36group. All surviving animals displayed significant acute cardiac dysfunction irrespective of rewarming method. Differences between groups in CPB exposure time or rewarming rate created no differences in need for vascular volume supply, in variables of cerebral metabolism, or in cerebral pressures and blood flow.

Conclusion:As 3 out of 8 animals did not survive weaning from CPB at 30°C, early weaning gave no advantages over weaning at 36°C. Further, in surviving animals, the results showed no differences between groups in the need for vascular volume replacement, nor any differences in cerebral blood flow or pressures. Most prominent, after weaning from CPB, was the existence of acute cardiac failure which was responsible for the inability to create an adequate perfusion irrespective of rewarming strategy.
Acute high altitude exposure, acclimatization and re-exposure on nocturnal breathing
Authors: Furian Michael , Bitos Konstantinos , Hartmann Sara E. , Muralt Lara , Lichtblau Mona , Bader Patrick R. , Rawling Jean M. , Ulrich Silvia , Poulin Marc J. , Bloch Konrad E.
Published: September 2022

Background: Effects of prolonged and repeated high-altitude exposure on oxygenation and control of breathing remain uncertain. We hypothesized that prolonged and repeated high-altitude exposure will improve altitude-induced deoxygenation and breathing instability.

Methods: 21 healthy lowlanders, aged 18-30y, underwent two 7-day sojourns at a high-altitude station in Chile (4–8 hrs/day at 5,050 m, nights at 2,900 m), separated by a 1-week recovery period at 520 m. Respiratory sleep studies recording mean nocturnal pulse oximetry (SpO2), oxygen desaturation index (ODI, >3% dips in SpO2), breathing patterns and subjective sleep quality by visual analog scale (SQ-VAS, 0–100% with increasing quality), were evaluated at 520 m and during nights 1 and 6 at 2,900 m in the 1st and 2nd altitude sojourn.

Results: At 520 m, mean ± SD nocturnal SpO2 was 94 ± 1%, ODI 2.2 ± 1.2/h, SQ-VAS 59 ± 20%. Corresponding values at 2,900 m, 1st sojourn, night 1 were: SpO2 86 ± 2%, ODI 23.4 ± 22.8/h, SQ-VAS 39 ± 23%; 1st sojourn, night 6: SpO2 90 ± 1%, ODI 7.3 ± 4.4/h, SQ-VAS 55 ± 20% (p < 0.05, all differences within corresponding variables). Mean differences (Δ, 95%CI) in acute effects (2,900 m, night 1, vs 520 m) between 2nd vs 1st altitude sojourn were: ΔSpO2 0% (-1 to 1), ΔODI -9.2/h (-18.0 to -0.5), ΔSQ-VAS 10% (-6 to 27); differences in acclimatization (changes night 6 vs 1), between 2nd vs 1st sojourn at 2,900 m were: ΔSpO2 -1% (-2 to 0), ΔODI 11.1/h (2.5 to 19.7), ΔSQ-VAS -15% (-31 to 1).

Conclusion: Acute high-altitude exposure induced nocturnal hypoxemia, cyclic deoxygenations and impaired sleep quality. Acclimatization mitigated these effects. After recovery at 520 m, repeated exposure diminished high-altitude-induced deoxygenation and breathing instability, suggesting some retention of adaptation induced by the first altitude sojourn while subjective sleep quality remained similarly impaired.
Predicting cigarette use initiation and dependence in adolescence using an affect-driven exploration model
Authors: Khurana Atika , Loan Christopher M. , Romer Dan
Published: September 2022

Adolescent decisions, especially in novel contexts, are often guided by affective evaluations (i.e., feelings associated with a stimulus) rather than knowledge of the risks and probabilities of different outcomes. In this study, we used the affect-driven exploration (ADE) model to illustrate how affective evaluations can play a critical role in driving early use of cigarettes, as well as the adaptive function of the resulting experiential learning in informing future affect and cigarette use. We analyzed five waves of data collected from a large, diverse community sample of adolescents who were followed from early to late adolescence (N = 386; 50.9% female; Baseline age = 11.41 ± 0.88 years) during years 2004–2010 to model trajectories of positive affect and risk perceptions (associated with cigarette use) and examined the associations of these trajectories with their self-reported cigarette use and dependence symptoms. Consistent with the ADE model, early initiators reported higher levels of positive affect at baseline, which we argue may have led them to try cigarettes. Notably, most early initiators reported a decline in positive affect over time, suggesting an experience-based shift in affective evaluations associated with cigarette use. Risk perceptions associated with cigarette use did not emerge as a significant predictor of cigarette use or subsequent dependence. Therefore, for deterring adolescent cigarette use, efforts to influence affect (through graphic warning labels and other media) may be more effective than directly influencing risk perceptions. Despite the affective basis for initiating cigarette use, few adolescents engaged in early use (N = 20) or developed symptoms of dependence (N = 25). Majority of those who engaged in early cigarette use showed a decline in positive affect, with corresponding increase in risk perceptions over time. Some early users may indeed continue to engage in cigarette use, but this is likely driven by the addictive properties of the drug. Overall these findings challenge the popular stereotype of impulsive and emotionally reactive behaviors during adolescence, and suggest a more nuanced interpretation of adolescent risk behavior.
Optimization of e-commerce logistics service quality considering multiple consumption psychologies
Authors: Ma Meng , Shen Lu , Sun XuanQing
Published: September 2022

This work is developed to improve the current quality of e-commerce logistics services. From the perspective of multiple consumer psychology, based on e-commerce, consumer psychology, and other related theories, vegetable e-commerce B is selected as the research object. The commodity quality, accuracy, and timeliness of commodity distribution and other factors of e-commerce B are discussed through questionnaire survey. Then, according to customers’ opinions about “e-commerce B’s distribution and professional aspects that need to be improved,” the research is conducted. Finally, the direction of follow-up optimization is proposed from four different perspectives of multiple consumption psychology. The research results show that more than 89% of the surveyed customers believe that e-commerce B does a good job in terms of commodity quality, accuracy, and timeliness of commodity distribution, and has a high level of logistics service. However, 26.34% of customers hope that e-commerce B can strengthen the protection of personal privacy, 24.97% hope that the platform can add “delay insurance” for goods, and 39.54% hope that the logistics information of purchased goods can be updated in real time. Therefore, e-commerce B needs to be optimized and improved continuously in the future development. Therefore, research on the optimization of logistics service quality of e-commerce is performed under multiple consumption psychology, which provides certain help for the rapid development of subsequent e-commerce.
Information needs and development of a question prompt sheet for upper extremity vascularized composite allotransplantation: A mixed methods study
Authors: Gacki-Smith Jessica , Kuramitsu Brianna R. , Downey Max , Vanterpool Karen B. , Nordstrom Michelle J. , Luken Michelle , Riggleman Tiffany , Altema Withney , Fichter Shannon , Cooney Carisa M. , Dumanian Greg A. , Jensen Sally E. , Brandacher Gerald , Tintle Scott , Levan Macey , Gordon Elisa J.
Published: September 2022

Background

People with upper extremity (UE) amputations report receiving insufficient information about treatment options. Furthermore, patients commonly report not knowing what questions to ask providers. A question prompt sheet (QPS), or list of questions, can support patient-centered care by empowering patients to ask questions important to them, promoting patient-provider communication, and increasing patient knowledge. This study assessed information needs among people with UE amputations about UE vascularized composite allotransplantation (VCA) and developed a UE VCA-QPS.

Methods

This multi-site, cross-sectional, mixed-methods study involved in-depth and semi-structured interviews with people with UE amputations to assess information needs and develop a UE VCA-QPS. Qualitative data were analyzed by thematic analysis; quantitative data were analyzed by descriptive statistics. The initial UE VCA-QPS included 130 items across 18 topics.

Results

Eighty-nine people with UE amputations participated. Most were male (73%), had a mean age of 46 years, and had a unilateral (84%) and below-elbow amputation (56%). Participants desired information about UE VCA eligibility, evaluation process, surgery, risks, rehabilitation, and functional outcomes. After refinement, the final UE VCA-QPS included 35 items, across 9 topics. All items were written at a ≤ 6th grade reading level. Most semi-structured interview participants (86%) reported being ‘completely’ or ‘very’ likely to use a UE VCA-QPS.

Conclusion

People with UE amputations have extensive information needs about UE VCA. The UE VCA-QPS aims to address patients’ information needs and foster patient-centered care. Future research should assess whether the UE VCA-QPS facilitates patient-provider discussion and informed decision-making for UE VCA.
A beam-tracking framework for THz networks
Authors: Stratidakis Giorgos , Droulias Sotiris , Alexiou Angeliki
Published: September 2022

Millimeter wave (mmWave) and terahertz (THz) frequencies are attractive for increased bandwidth applications, however are vulnerable to blockage and suffer from high pathloss. While the use of directional antennas can potentially mitigate these effects, the need for careful alignment becomes crucial, especially when the user moves. In this context, to ensure a reliable link, several parameters must be taken into account, such as the type of user’s motion, the location of the access point (AP), the shape of the area, the beamwidth, etc. In this work, the link reliability is divided into two main categories, the trajectory tracking resolution and the angular resolution. To address the challenges of both categories, a beam-tracking algorithm that promises high tracking reliability and low pilot overhead is proposed. The algorithm employs multiple cooperating APs and a hierarchical codebook and the performance of the proposed tracking method is evaluated through Monte-Carlo simulations with the probability of success, the average number of pilots per timeslot and the mean square error (MSE) as metrics, for different tracking estimation frequencies and different number of blocked links.
Analysis of the influencing factors on fine-needle aspiration biopsy results of the thyroid
Authors: Wang Chun-Yan , Zhou Yan , Ren Yu-Yuan , Luan Yu-Shuang , Jiang Zhong-Cai , Wang Zhi-Xing
Published: September 2022

Objective

The objective of the study is to analyze the influencing factors on the results of thyroid fine-needle aspiration biopsy (FNAB).

Method

A total of 339 patients who underwent FNAB in our hospital from December 2018 to July 2021 were retrospectively selected. The patients were chosen according to the gender ratio, age, and thyroid ultrasound characteristics and were divided into three groups: (1) a 22G needle vacuum aspiration group (Group 1, n = 85), (2) a 22G biopsy needle non-vacuum aspiration group (Group 2, n = 50), and (3) a 25G biopsy needle non-vacuum aspiration group (Group 3, n = 204). Patients in these groups were evaluated for determining the FNAB dissatisfaction rate of pathological samples. A bivariate regression analysis of independent risk factors related to the unsatisfactory pathological diagnosis of samples was performed.

Results

The specimen dissatisfaction rates of the three groups were 22/85 (25.9%), 15/50 (30%), and 18/186 (9.7%), respectively. The overall sample dissatisfaction rate was 55/339 (16.2%), and the sample satisfaction rate of Group 3 was higher than that of Groups 1 and 2 (P < 0.05). Logistic bivariate regression analysis showed that the age of the patients and the capillary sampling needles and aspiration methods were two independent risk factors for determining the dissatisfaction rate of FNAB pathological samples.

Conclusion

A 25G capillary sampling aspiration biopsy needle was selected to perform FNAB in thyroid nodules without vacuum aspiration, which could effectively improve the accuracy of FNAB results with a high specimen satisfaction rate.
Perioperative variation in serum FGF-23 level and its correlation with MACCE risk in unprotected left main coronary artery disease patients receiving coronary artery bypassing grafting
Authors: Wang Fanshun , Ma Runhua , Wang Chunsheng
Published: September 2022

Objective

Fibroblast growth factor-23 (FGF-23) mediates vascular endothelial injury, inflammatory infiltration, and atherosclerosis, which could reflect major adverse cardiac and cerebrovascular event (MACCE) risk in several cardiovascular diseases. This study aims to further investigate the perioperative change of FGF-23, as well as its association with clinical characteristics and MACCE risk in unprotected left main coronary artery disease (ULMCAD) patients receiving coronary artery bypass grafting (CABG).

Methods

A total of 226 ULMCAD patients who underwent CABG were enrolled. Serum samples of the patients were collected on the day before CABG, the third day (D3) after CABG, and at discharge; then, the FGF-23 level was determined by enzyme-linked immunosorbent assay. The MACCE rate was recorded during a median follow-up of 25.5 (range: 2.0–46.0) months.

Results

The median, interquartile range (IQR), and range of FGF-23 level in ULMCAD patients receiving CABG were 717.0, 582.5–869.8, and 407.0–1765.0 pg/ml, respectively. FGF-23 level was increased in patients with both previous heart failure (P = 0.046) and chronic renal failure (P = 0.009) compared to those without. FGF-23 level increased from before surgery [median (IQR): 712.5 (574.5–879.8) pg/ml] to D3 [median (IQR): 844.0 (666.0–1072.5) pg/ml], then declined at discharge [median (IQR): 764.5 (569.3–986.8) pg/ml] (P < 0.001). Meanwhile, the preoperative FGF-23 level (P = 0.028), but not the FGF-23 level at discharge (P = 0.067) was positively correlated with the cumulative MACCE rate. Multivariable Cox's analyses found that preoperative FGF-23 level could independently predict cumulative MACCE rate [P = 0.015, hazards ratio (HR) = 2.940].

Conclusion

Preoperative FGF-23 level predicts higher MACCE risk in ULMCAD patients undergoing CABG surgery.
Novel three-dimensional acellular dermal matrix for prepectoral breast reconstruction: First year in review with BRAXON®Fast
Authors: Berna Giorgio , De Grazia Alessia , Antoniazzi Elisa , Romeo Marco , Dell’Antonia Francesco , Lovero Stefano , Marchica Paolo , Rizzetto Christian , Burelli Paolo
Published: September 2022

Implant-based breast reconstruction is part of breast cancer treatment, and increasingly optimized reconstructive procedures exploit highly biocompatible materials to ensure enhanced aesthetic-functional results. Acellular dermal matrices (ADMs) are collagen-based materials that made prepectoral implant placement possible, thanks to their bioactive antifibrosis action. Recently, the first three-dimensional ADM, BRAXON®Fast, has been produced. Its 3D design represents the technological evolution of BRAXON® ADM, a flat collagen matrix, and allows for a time-saving complete wrapping of the synthetic prosthesis, thus creating a total biological interface on the implant with patient's tissues. Here, we report our experience on the first 23 eligible patients who received BRAXON®Fast-assisted prepectoral reconstruction. On a total of 27 breasts, the overall complication rate was 11.1%, including one minor seroma (3.7%), one case of necrosis (3.7%), and one implant removal due to infection. As new-generation devices, 3D ADMs showed an effective performance, allowing to reduce the overall exposure time for implant preparation and providing an optimal safety profile.
Optimal immediate sagittal alignment for kyphosis in ankylosing spondylitis following corrective osteotomy
Authors: Luo Jianzhou , Yang Kai , Yang Zili , Feng Chaoshuai , Li Xian , Luo Zhenjuan , Tao Huiren , Duan Chunguang , Wu Tailin
Published: September 2022

Purpose

To investigate the optimal immediate sagittal alignment of kyphosis in ankylosing spondylitis (AS) following corrective osteotomy.

Methods

Seventy-seven AS patients who underwent osteotomy were enrolled. Radiographic parameters, including global kyphosis (GK), lumbar lordosis (LL), T1 spinopelvic inclination (T1SPI), sagittal vertical axis (SVA), T1 pelvic angle (TPA), pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), and PI and LL mismatch (PI–LL), were collected. The clinical outcome was evaluated using the Scoliosis Research Society-22 (SRS-22) questionnaire and Oswestry Disability Index (ODI). At the final follow-up, SVA > 5 cm was regarded as sagittal imbalance, and a total ODI ≤ 20 or total SRS-22 score ≥4 was considered to indicate a good clinical outcome.

Results

Seventy-seven patients with an average age of 37.4 ± 8.6 years were followed up for 29.4 ± 4.2 months. At the final follow-up, GK, LL, PT, SS, TPA, and T1SPI showed some degree of correction loss (P < 0.05). The follow-up parameters could be predicted with the immediate postoperative parameters through their linear regression equation (P < 0.05). The postoperative immediate T1SPI, TPA, SVA, and PI were also highly correlated with the clinical outcome (ODI and/or SRS-22) at the final follow-up (P < 0.05). Based on the relationship, the optimal immediate sagittal alignment for obtaining good clinical outcome was determined: T1SPI ≤ 0.9°, TPA ≤ 31.5°, and SVA ≤ 9.3cm. AS patients with PI ≤ 49.2° were more likely to achieve the optimal alignment and obtained lower ODI and a lower incidence of sagittal imbalance than those with PI > 49.2° at the final follow-up (P < 0.05).

Conclusion

Postoperative immediate parameters could be used to predict the final follow-up parameters and clinical outcome. The optimal postoperative immediate sagittal alignment of AS patients was T1SPI ≤ 0.9°, TPA ≤ 31.5°, and SVA ≤ 9.3 cm, providing a reference for kyphosis correction and a means for clinical outcome evaluation. Patients with a lower PI (≤49.2°) were more likely to achieve optimal alignment and obtain satisfactory clinical outcomes.
Surgeons' workload assessment during indocyanine-assisted deep endometriosis surgery using the surgery task load index: The impact of the learning curve
Authors: Spagnolo Emanuela , Cristóbal Quevedo Ignacio , Gortázar de las Casas Sara , López Carrasco Ana , Carbonell López Maria , Pascual Migueláñez Isabel , Hernández Gutiérrez Alicia
Published: September 2022

Objective

Assess the surgeons' workload during deep endometriosis surgery after ureteral ICG

Design

Prospective, consecutive, comparative, single-center study

Population

41 patients enrolled to deep endometriosis surgery with ureteral ICG from January 2019 to July 2021 at La Paz University Hospital

Methods

Patients were divided into 2 groups: patients operated during the learning curve of ureteral ICG instillation and patients operated after the technique was implemented and routinely performed. After surgery, the SURG-TLX form was completed by the surgeons. We evaluated whether a workload reduction occurred.

Main outcomes measures

Surgeon's workload was measured using the SURG-TLX form, obtaining the total workload and 6 different dimensions (distractions, temporal demands, task complexity, mental demands, situational stress and physical demands)

Results

A significant positive correlation was found between surgical complexity and situational stress (p = 0.04). Mental demands (p = 0.021), physical demands (p = 0.03), and total workload (p = 0.025) were significantly lower when the technique was routinely performed. The mental demand, physical demands, and total workload perceived by the surgeons at the beginning of the implementation was higher (68 [39–72], 27 [11–46.5], 229 [163–240], respectively) than in the latter ones (40 [9–63], 11.5 [0–32.8], 152 [133.3–213.8], respectively). Distractions appeared to be higher in the latter surgeries (8.5 [0–27.8]) than in the first surgeries (0 [0–7]; p = 0.057).

Conclusions

Ureter ICG instillation prior to DE surgery significantly reduces the mental and physical demands and total workload of the surgeons in DE surgeries after overcoming the learning curve. Distractions appear to increase as surgical stress decreases.
Pre-validation of a reporter gene assay for oxidative stress for the rapid screening of nanobiomaterials
Authors: Martin Sebastin , de Haan Laura , Miro Estruch Ignacio , Eder Kai Moritz , Marzi Anne , Schnekenburger Jürgen , Blosi Magda , Costa Anna , Antonello Giulia , Bergamaschi Enrico , Riganti Chiara , Beal David , Carrière Marie , Taché Olivier , Hutchison Gary , Malone Eva , Young Lesley , Campagnolo Luisa , La Civita Fabio , Pietroiusti Antonio , Devineau Stéphanie , Baeza Armelle , Boland Sonja , Zong Cai , Ichihara Gaku , Fadeel Bengt , Bouwmeester Hans
Published: September 2022

Engineered nanomaterials have been found to induce oxidative stress. Cellular oxidative stress, in turn, can result in the induction of antioxidant and detoxification enzymes which are controlled by the nuclear erythroid 2-related factor 2 (NRF2) transcription factor. Here, we present the results of a pre-validation study which was conducted within the frame of BIORIMA (“biomaterial risk management”) an EU-funded research and innovation project. For this we used an NRF2 specific chemically activated luciferase expression reporter gene assay derived from the human U2OS osteosarcoma cell line to screen for the induction of the NRF2 mediated gene expression following exposure to biomedically relevant nanobiomaterials. Specifically, we investigated Fe3O4-PEG-PLGA nanomaterials while Ag and TiO2 “benchmark” nanomaterials from the Joint Research Center were used as reference materials. The viability of the cells was determined by using the Alamar blue assay. We performed an interlaboratory study involving seven different laboratories to assess the applicability of the NRF2 reporter gene assay for the screening of nanobiomaterials. The latter work was preceded by online tutorials to ensure that the procedures were harmonized across the different participating laboratories. Fe3O4-PEG-PLGA nanomaterials were found to induce very limited NRF2 mediated gene expression, whereas exposure to Ag nanomaterials induced NRF2 mediated gene expression. TiO2 nanomaterials did not induce NRF2 mediated gene expression. The variability in the results obtained by the participating laboratories was small with mean intra-laboratory standard deviation of 0.16 and mean inter laboratory standard deviation of 0.28 across all NRF2 reporter gene assay results. We conclude that the NRF2 reporter gene assay is a suitable assay for the screening of nanobiomaterial-induced oxidative stress responses.
Entrepreneurial Management as a Predictor of Business Performance in the Greater Accra Region, Ghana
Authors: Nti Nicholas Kofi
Published: September 2022

Objective: This study used the Behavioural theory of entrepreneurship as the theoretical foundation, and correlation research design to examine the impact of the entrepreneurial management practices (strategic orientation, resource orientation, management structure, reward philosophy, growth orientation, and entrepreneurial culture) on business performance in Ghana. Research Design & Methods: 500 businesses in the Greater Accra Region were selected for the data analysis. Descriptive statistics and hierarchical multiple regression were used to analyse the data. Findings: After controlling the business profile, the result showed that four out of the six entrepreneurial management practices – resource orientation, strategic orientation, reward philosophy, and entrepreneurial culture significantly predicted business performance. However, growth orientation and management structure did not. Implications & Recommendations: The study concludes that entrepreneurial management practices improve business performance in Ghana. The researcher recommends that approaching the study with a mixed-method will help provide a holistic understanding of the management decision-making of the entrepreneurs and its impact on their business performance. Contribution & Value Added: This study contributes to the entrepreneurial management literature in Ghana.
Photothermal Nano-Vaccine Promoting Antigen Presentation and Dendritic Cells Infiltration for Enhanced Immunotherapy of Melanoma via Transdermal Microneedles Delivery
Authors: Zhu Jiaojiao , Chang Ruimin , Wei Benliang , Fu Yao , Chen Xiang , Liu Hong , Zhou Wenhu
Published: September 2022

Immunotherapy has demonstrated the potential to cure melanoma, while the current response rate is still unsatisfactory in clinics. Extensive evidence indicates the correlation between the efficacy and pre-existing T-cell in tumors, whereas the baseline T-cell infiltration is lacking in low-response melanoma patients. Herein, we demonstrated the critical contribution of dendritic cells (DCs) on melanoma survival and baseline T-cell level, as well as the efficacy of immunotherapy. Capitalized on this fact, we developed a photothermal nano-vaccine to simultaneously promote tumor antigens presentation and DCs infiltration for enhanced immunotherapy. The nano-vaccine was composed of polyserotonin (PST) core and tannic acid (TA)/Mn
2+
coordination-based metal-organic-framework (MOF) shell for
β
-catenin silencing DNAzyme loading, which was further integrated into dissolving microneedles to allow noninvasive and transdermal administration at melanoma skin. The nano-vaccine could rapidly penetrate skin upon microneedles insertion and exert a synergistically amplified photothermal effect to induce immunogenic cell death (ICD). The MOF shell then dissociated and released Mn
2+
as a cofactor to self-activate DNAzyme for
β
-catenin suppression, which in turn caused a persistent CCL4 excretion to promote the infiltration of DCs into the tumor. Meanwhile, the liberated PST core could effectively capture and facilitate tumor antigens presentation to DCs. As a result, potent antitumor efficacies were achieved for both primary and distal tumors without any extra treatment, indicating the great promise of such a nano-vaccine for on-demand personalized immunotherapy of melanoma.
THE USE OF LINEAR MODELS OF THE MECHANICAL SYSTEM OF THE MACHINE’S TO STUDY THE OPERATION OF THEIR ACTUATORS
Authors: Meshcheryakov Alexander V. , Bogacheva Svetlana Yu.
Published: September 2022

This article discusses the possibility of using chain linear models to study the kinematics of the movement of actuators of machines with a spatial branched mechanical system. The example of weaving machines shows the effect on kinematics of movement of links of their inertial and dissipative characteristics, driving moment, torque developed by the starting clutch, rigidity of transmission mechanisms. During the studies, chain dynamic models with rigid links, which are connected by elastic dissipative bonds, second-order Lagrange's equations were used.
NEW APPROACHES TO THERMAL CALCULATIONS OF VENTILATED CLOTHING
Authors: Rodicheva Margarita V. , Abramov Anton V. , Utkin Nikolai N. , Malyarova Polina M.
Published: September 2022

It is shown that the existing mathematical models of heat transfer do not fully take into account the real shape and dimensions of the air layers of ventilated clothing. A numerical modelling method is proposed that takes into account the influence of the morphology of the human body and the physical and mechanical properties of textile materials on the size and shape of the air gap. Peculiarities of convective processes in the air interlayers of ventilated clothing at the ambient temperature of +27 °C are revealed by the calcula-tions. The zones of intensification and deceleration of the air flow under the clothes were revealed. It is shown that the results of calculations are consistent with the results of experimental studies.
EVALUATION OF STEAM PERMEABILITY OF MATERIALS SYSTEMS FOR ADAPTIVE CLOTHING OF PEOPLE WITH LIMITED MOTOR CAPABILITIE
Authors: Zimina Marina V. , Chagina Lyubov′ L. , Ivanov Vladislav V.
Published: September 2022

The article presents the results of a study of the characteristics of vapour permeability of materials systems for adaptive clothing of people with motor disabilities. The expediency of experimental evaluation of these indicators for the modern range of jacket fabrics of this target audience has been established. The analysis of the results showed that the value of vapour permeability depends on the structural characteristics and fibrous composition of the material systems and varies in a wide range. A comparative analysis of the characteristics of the vapour permeability of the material systems allows, during confection, to choose a ra-tional option for creating garments that are characterised by high consumer value from the point of view of providing comfortable microclimate conditions of the space under the clothes during operation. The results of experimental studies can be used at the design stage to predict the characteristics of vapour permeability of systems of materials of the jacket assortment of clothing for people with disabilities.
PRODUCTION OF ELECTRICALLY CONDUCTIVE TEXTILE MATERIALS LAMINATED WITH POLYMER FILMS
Authors: Karnoukhov Alexander E. , Timoshina Yulia A. , Voznesensky Emil F.
Published: September 2022

The article presents the results of a study of the complex properties of metallised textile materials laminated with polymer films by thermal pressing. The application of a copper metal coating to the surface of a textile material was carried out by magnetron sputtering. To increase the adhesion of synthetic textile materials to the metal coating, pretreatment in plasma of a radio-frequency capacitive (RFC) discharge of reduced pressure was used, carried out immediately before spraying the metal within one technological cycle. It has been established that the metallised textile materials obtained have a shielding coefficient of up to 50 dB, and the use of perforated films for lamination makes it possible to obtain materials with air and vapour permeability. The use of plasma modification in the process makes it possible to increase the adhesion of polymer materials to metal coatings, which contributes to an increase in the wear resistance of the resulting textile materials with shielding properties.
Subdiaphragmatic Abscess: Complication of Emergency Laparotomy
Authors: Kharche Abhijeet Dilip , Iyer Sriranjani , Natarajan Saileshwar
Published: September 2022

Intra-abdominal abscesses usually occur following any intra-abdominal surgery, trauma, Gastrointestinal infection or intestinal perforation. In particular, the diagnosis of sub-phrenic collection can be notoriously difficult. This fact is expressed by the well-known aphorism: 'Pus somewhere, pus nowhere else, pus under the diaphragm'. Sub-diaphragmatic abscesses form between the diaphragm and abdominal organs, such as the liver and spleen. Depending on the severity of the sub-diaphragmatic abscess and the cause, treatment method may vary for each case. The abscess may be treated with early percutaneous drainage and empiric intravenous antibiotics. When dealing with post-operative persistent pyrexia that does not react to antibiotics, surgeons must always be careful, and the likelihood of a sub-diaphragmatic abscess must always be considered. If not treated, thoracic and abdominal complications may prevail, in rare cases, death. We present a case of sub-diaphragmatic abscess in a patient with a perforated duodenal ulcer treated by ultrasound-guided percutaneous drainage with a good outcome.
TINJAUAN UNDANG - UNDANG NO.18 TAHUN 2011 TENTANG KOMISI YUDISIAL GUNA TERWUJUD PERADILAN YANG BERSIH DI PENGADILAN NEGERI SEMARANG
Authors: Ibnu Sefrin
Published: September 2022

Penegakan keadilan sebagai unsur penting dari hak asasi manusia, sejatinya harus dibangun dan diwujudkan dalam sistem negara hukum manapun. Selain menghadirkan sistem dan personil peradilan yang berkualitas, juga perlu didukung dengan mekanisme perekrutan dan pembinaan hakim serta penegak hukum lainnya yang jujur dan berintegritas. komisi yudisial yang secara resmi diberikan mandat oleh konstitusional dan Undang-Undang No. 18 Tahun 2011 untuk mewujudkan kewibawaan peradilan sehingga akan dipercaya kembali oleh masyarakat tersebut.2

Komisi Yudisial yang lahir karena adanya reformasi memang bertujuan mewujudkan peradilan yang bersih, mandiri, independen, transparan serta menegakan keadilan untuk semua lapisan masyarakat. Terlebih lagi, konstitusi sudah jelas menyebut jika wewenang utama KY adalah menjaga dan menegakkan kehormatan, keluhuran martabat, dan perilaku hakim.

Penerapan UU No.18 Tahun 2011 tentang Komisi Yudisial dalam terwujudnya peradilan bersih di PN Semarang dilakukan dengan membentuk penghubung komisi yudisial di daerah, dengan cara inilah komisi yudisial melaksanakan tugas dan fungsinya sebagai penghubung demi terciptanya sistem pengadilan bersih di PN Semarang. Hal itu sesuai dengan Pasal 3 ayat (2) Undang- undang Nomor 18 Tahun 2011 tentang Komisi Yudisial yang berbunyi “Komisi Yudisal dapat mengangkat penghubung di daerah sesuai dengan kebutuhan”. Bahwa wewenang Komisi Yudisial dalam hal pengawasan kepada hakim hanya sebatas memberikan rekomendasi kepada Mahkamah Agung terkait pemberian sanksi. Diperlukan kesepakatan bersama lembaga peradilan bagaimana meningkatkan kapasitas wewenang komisi yudisial agar lebih berperan dalam menegakkan peradilan yang bersih dan berwibawa. Dibutuhkan juga partisipasi aktif masyarakat dalam melakukan pemantauan dengan melakukan kerja sama dengan Komisi Yudisial melalui pos koordinasi pemantauan peradilaan. Skripsi ini diharapkan mampu menjadi bahan pertimbangan Komisi Yudisial agar dapat menambah jumlah petugas penghubung di daerah demi efektifitas kerja dan terwujudnya cita-cita Peradilan bersih di Pengadilan Negeri Semarang.

Kata kunci:, Komisi Yudisial, Peradilan Bersih Hakim
PELATIHAN BANTUAN HIDUP DASAR UNTUK MENINGKATKAN PENGETAHUAN DAN KETERAMPILAN PENANGGULANGAN GAWAT DARURAT PADA RELAWAN PALANG MERAH INDONESIA
Authors: Pujianto Ahmat , Ose Maria Imaculata , Tri Wahyudi Donny , Hidaya Nurman , Handayani Fitriya
Published: September 2022

Angka kejadian henti jantung di luar rumah sakit (out of hospital cardiac arrest) cenderung tinggi dan kurang dari 40% dari jumlah korban tersebut diberikan tindakan resusitasi jantung paru (RJP) oleh masyarakat awam. Kondisi tersebut menunjukkan bahwa keterampilan bantuan hidup dasar sangat penting dimiliki oleh masyarakat termasuk juga para relawan. Kesiapsiagaan relawan yang didukung dengan kompetensi yang mumpuni menjadi faktor penting bagi relawan dalam memberikan pertolongan pertama. Pentingnya pengetahuan dan keterampilan bantuan hidup dasar di masyarakat, maka masyarakat termasuk para relawan perlu diberikan pelatihan bantuan hidup dasar. Kegiatan ini diikuti oleh 15 peserta yang merupakan relawan PMI. Kegiatan ini meliputi 2 tahap, diantaranya penyampaian teori/konsep BHD dengan metode ceramah, serta praktik melakukan resusitasi jantung paru (RJP) dan manajemen pembebasan jalan napas pada korban tersedak/chocking. Secara kuantitatif terdapat perbedaan nilai pre dan post test pengetahuan peserta tentang BHD. Untuk aspek keterampilan, rerata nilai peserta setelah pelatihan BHD juga baik. Pelatihan BHD mampu meningkatkan pengetahuan dan keterampilan peserta.
SKRINING KAKI DIABETES “IPSWICH TOUCH TEST (IpTT)” DALAM MENDETEKSI RESIKO LUKA KAKI PADA PASIEN DM
Authors: Sulistiani Ita , Djamaluddin Nurdiana , Rahim Nirwanto K
Published: September 2022

Penatalaksanaan penyandang DM perlu melibatkan keluarga sebagai support system, tidak hanya dalam proses pengontrolan gula darah tetapi juga untuk mencegah terjadinya kejadian LKD.Bukti menunjukkan metode yang paling efektif untuk mengurangi peningkatan gejala dari neuropati yang berakibat pada angka kejadian LKD yang terus meningkat adalah dengan melakukan skrining neuropati. Skrining untuk gejala dan tanda-tanda neuropati diabetes penting dalam praktek klinis, karena dapat mendeteksi tahap awal neuropati, yang memungkinkan dilakukannya intervensi awal. Terdapat pemeriksaan sederhana dengan menggunakan Ipswich Touch Test (IpTT), yang dilakukan dimana saja, kapanpun pada pasien DM.IpTT juga dapat diaplikasikan secara langsung oleh tenaga non profesional dan tidak memerlukan alat khusus dan hanya memerlukann sedikit latihan saja. Sehingga dapat diajarkan oleh keluarga yang merupakan orang terdekat dari pasien. Adapun metode yang digunakan dalam program ini adalah sosialisasi yang diberikan pada keluarga pasien . Program ini bekerja sama dengan Puskesmas Kota Selatan. Alur kegiatan meliputi tahap persiapan berupa observasi ke puskesmas dan melakukan penyuluhan dan sosialisai terhadap keluarga pasien DM terkait cara melakukan skrining awal menggunakan IpTT. Dengan adanya partisipasi langsung olehkeluarga, diharapkan dapat mengurangi angka kejadian LKD
PENYULUHAN STIMULASI PERKEMBANGAN PSIKOSOSIAL REMAJA PUTRI DI PESANTREAN ALISYAF KABUPATEN PEKALONGAN
Authors: Kamalah Aisyah Dzil , Arifiyanto Dafid , Salma Ayesha Nur
Published: September 2022

Masalah psikososial yang sering terjadi pada remaja putri adalah kurang percaya diri, merasa malu, mudah tersinggung dan marah. Hal ini akan menghambat remaja putri untuk tumbuh , berkembang dan mengeksplorasi kemampuannya. Sehingga perlu dilakukan penyuluhan tentang stimulasi perkembangan psikososial pada remaja putri di Pesantren Alisyaf Kabupaten Pekalongan. Kegiatan diikuti oleh 66 remaja putri / santriwati. Kegiatan yang dilakukan dibagi mejdai dua sesi. Sesi pertama adalah dengan penyampaian materi terkait perkembangan psikososial dan sesi kedua adalah dengan konsultasi secara langsung. Kegiatan penyampaian materi disampaikan menggunakan media power point yang ditampilkan dalam layar proyektor dilakukan selama 30 menit. Sesi kedua adalah konsultasi dilakukan selama 90 menit. Perlu adanya tindak lanjut terkait peningkatan kesehatan jiwa remaja putri untuk mengatasi masalah psikososial yang terjadi pada remaja putri di lingkungan pesantren Alisyaf Kabupaten Pekalonga
ANALYSING HOUSE PRICE PREDICTIONS ACCORDING TO LIVING STANDARDS BASED ON MACHINE LEARNING METHODS
Authors: Mahboob Khalid , Khalil Nida , Rehan Saniah
Published: September 2022

There is a lack of reliable economical methods for forecasting house prices for those who wish to buy a house according to their living standards. This paper presents details of predictive analytics for house pricing in three different towns of Karachi, Pakistan according to different living standards based on machine learning (ML) methods. The purpose of this study is to determine which data set features contribute greatly to the accuracy of the predictions when experimenting with selected predictive techniques. The house price value has been analysed using five different ML methods. A model selection has been made by comparing the accuracy of the techniques based on some performance metrics and the best technique was used to predict the house price value.
Implikasi Hukum Bagi Akta Pembiayaan Murabahah yang Tidak Sesuai dengan Prinsip Syariah
Authors: Ayatik Arini , Ochtorina Susanti Dyah , Indra Tektona Rahmadi
Published: September 2022

Pada perbankan syariah terdapat pembiayaan salah satunya murabahah. Semua produk bank syariah harus berdasarkan pada prinsip syariah. Akad murabahah harus dibuat dengan akta otentik. Akta tersebut harus sesuai dengan prinsip syariah. Penelitian ini menganalisis mengenai akibat hukum bagi akta pembiayaan murabahah yang tidak sesuai dengan prinsip syariah. Adapun tujuan penelitian ini yaitu untuk mengetahui dan menganalisis akibat hukum bagi akta pembiayaan murabahah yang tidak sesuai dengan prinsip syariah. Penelitian ini menggunakan metode yuridis normatif, dengan empat pendekatan masalah yaitu pendekatan perundang-undangan, pendekatan konseptual, pendekatan kasus, dan pendekatan perbandingan. Hasil penelitian ini yaitu apabila akta pembiayaan murabahah tidak sesuai dengan prinsip syariah maka akta tersebut dapat batal demi hukum.