bluuebunny/crossref_metadata_2025 huggingface dataset:
How Relevant Is It to Use Mineral Proxies to Mimic the Atmospheric Reactivity of Natural Dust Samples? A Reactivity Study Using SO2 as Probe Molecule
Authors: Urupina Darya , Romanias Manolis N. , Thevenet Frederic
Published: March 2021

The experimental investigation of heterogeneous atmospheric processes involving mineral aerosols is extensively performed in the literature using proxy materials. In this work we questioned the validity of using proxies such as Fe2O3, FeOOH, Al2O3, MgO, CaO, TiO2, MnO2, SiO2, and CaCO3 to represent the behavior of complex mixtures of minerals, such as natural desert and volcanic dusts. Five volcanic dusts and three desert dusts were compared to a number of metal oxides, commonly used in the literature to mimic the behavior of desert dusts in the ability to form sulfites and sulfates on the surface exposed to SO2 gas. First, all samples were aged at room temperature, atmospheric pressure, under controlled experimental conditions of 175 ppm SO2 for 1 h under 30% of relative humidity. Second, they were extracted with 1% formalin and analyzed by High-Performance Liquid Chromatography (HPLC) to quantify and compare the amount of sulfites and sulfates formed on their surfaces. It was evidenced that under the experimental conditions of this study neither one selected pure oxide nor a mixture of oxides can adequately typify the behavior of complex mixtures of natural minerals. Therefore, to evaluate the real-life impact of natural dust on atmospheric processes it is of vital importance to work directly with the natural samples, both to observe the real effects of desert and volcanic dusts and to evaluate the relevancy of proposed proxies.
A Metabolomic Approach to Beer Characterization
Authors: Cavallini Nicola , Savorani Francesco , Bro Rasmus , Cocchi Marina
Published: March 2021

The consumers’ interest towards beer consumption has been on the rise during the past decade: new approaches and ingredients get tested, expanding the traditional recipe for brewing beer. As a consequence, the field of “beeromics” has also been constantly growing, as well as the demand for quick and exhaustive analytical methods. In this study, we propose a combination of nuclear magnetic resonance (NMR) spectroscopy and chemometrics to characterize beer. 1H-NMR spectra were collected and then analyzed using chemometric tools. An interval-based approach was applied to extract chemical features from the spectra to build a dataset of resolved relative concentrations. One aim of this work was to compare the results obtained using the full spectrum and the resolved approach: with a reasonable amount of time needed to obtain the resolved dataset, we show that the resolved information is comparable with the full spectrum information, but interpretability is greatly improved.
Excited-State Intramolecular Proton Transfer: A Short Introductory Review
Authors: Joshi Hem C. , Antonov Liudmil
Published: March 2021

In this short review, we attempt to unfold various aspects of excited-state intramolecular proton transfer (ESIPT) from the studies that are available up to date. Since Weller’s discovery of ESIPT in salicylic acid (SA) and its derivative methyl salicylate (MS), numerous studies have emerged on the topic and it has become an attractive field of research because of its manifold applications. Here, we discuss some critical aspects of ESIPT and tautomerization from the mechanistic viewpoint. We address excitation wavelength dependence, anti-Kasha ESIPT, fast and slow ESIPT, reversibility and irreversibility of ESIPT, hydrogen bonding and geometrical factors, excited-state double proton transfer (ESDPT), concerted and stepwise ESDPT.
Special Issue “Lignocellulosic Biomass”
Authors: Rodríguez Alejandro , Espinosa Eduardo
Published: March 2021

The use of lignocellulosic biomass as potential raw material for fractionation and transformation into high value-added products or energy is gathering the attention of scientists worldwide in seeking to achieve a green transition in our production systems [...]
Design and Synthesis of Non-Covalent Imidazo[1,2-a]quinoxaline-Based Inhibitors of EGFR and Their Anti-Cancer Assessment
Authors: Kumar Manvendra , Joshi Gaurav , Arora Sahil , Singh Tashvinder , Biswas Sajal , Sharma Nisha , Bhat Zahid Rafiq , Tikoo Kulbhushan , Singh Sandeep , Kumar Raj
Published: March 2021

A series of 30 non-covalent imidazo[1,2-a]quinoxaline-based inhibitors of epidermal growth factor receptor (EGFR) were designed and synthesized. EGFR inhibitory assessment (against wild type) data of compounds revealed 6b, 7h, 7j, 9a and 9c as potent EGFRWT inhibitors with IC50 values of 211.22, 222.21, 193.18, 223.32 and 221.53 nM, respectively, which were comparable to erlotinib (221.03 nM), a positive control. Furthermore, compounds exhibited excellent antiproliferative activity when tested against cancer cell lines harboring EGFRWT; A549, a non-small cell lung cancer (NSCLC), HCT-116 (colon), MDA-MB-231 (breast) and gefitinib-resistant NSCLC cell line H1975 harboring EGFRL858R/T790M. In particular, compound 6b demonstrated significant inhibitory potential against gefitinib-resistant H1975 cells (IC50 = 3.65 μM) as compared to gefitinib (IC50 > 20 μM). Moreover, molecular docking disclosed the binding mode of the 6b to the domain of EGFR (wild type and mutant type), indicating the basis of inhibition. Furthermore, its effects on redox modulation, mitochondrial membrane potential, cell cycle analysis and cell death mode in A549 lung cancer cells were also reported.
Thermal Energy Storage and Heat Transfer of Nano-Enhanced Phase Change Material (NePCM) in a Shell and Tube Thermal Energy Storage (TES) Unit with a Partial Layer of Eccentric Copper Foam
Authors: Ghalambaz Mohammad , Mehryan Seyed Abdollah Mansouri , Ayoubloo Kasra Ayoubi , Hajjar Ahmad , El Kadri Mohamad , Younis Obai , Pour Mohsen Saffari , Hulme-Smith Christopher
Published: March 2021

Thermal energy storage units conventionally have the drawback of slow charging response. Thus, heat transfer enhancement techniques are required to reduce charging time. Using nanoadditives is a promising approach to enhance the heat transfer and energy storage response time of materials that store heat by undergoing a reversible phase change, so-called phase change materials. In the present study, a combination of such materials enhanced with the addition of nanometer-scale graphene oxide particles (called nano-enhanced phase change materials) and a layer of a copper foam is proposed to improve the thermal performance of a shell-and-tube latent heat thermal energy storage (LHTES) unit filled with capric acid. Both graphene oxide and copper nanoparticles were tested as the nanometer-scale additives. A geometrically nonuniform layer of copper foam was placed over the hot tube inside the unit. The metal foam layer can improve heat transfer with an increase of the composite thermal conductivity. However, it suppressed the natural convection flows and could reduce heat transfer in the molten regions. Thus, a metal foam layer with a nonuniform shape can maximize thermal conductivity in conduction-dominant regions and minimize its adverse impacts on natural convection flows. The heat transfer was modeled using partial differential equations for conservations of momentum and heat. The finite element method was used to solve the partial differential equations. A backward differential formula was used to control the accuracy and convergence of the solution automatically. Mesh adaptation was applied to increase the mesh resolution at the interface between phases and improve the quality and stability of the solution. The impact of the eccentricity and porosity of the metal foam layer and the volume fraction of nanoparticles on the energy storage and the thermal performance of the LHTES unit was addressed. The layer of the metal foam notably improves the response time of the LHTES unit, and a 10% eccentricity of the porous layer toward the bottom improved the response time of the LHTES unit by 50%. The presence of nanoadditives could reduce the response time (melting time) of the LHTES unit by 12%, and copper nanoparticles were slightly better than graphene oxide particles in terms of heat transfer enhancement. The design parameters of the eccentricity, porosity, and volume fraction of nanoparticles had minimal impact on the thermal energy storage capacity of the LHTES unit, while their impact on the melting time (response time) was significant. Thus, a combination of the enhancement method could practically reduce the thermal charging time of an LHTES unit without a significant increase in its size.
CdSe Quantum Dots in Human Models Derived from ALS Patients: Characterization, Nuclear Penetration Studies and Multiplexing
Authors: Tosat-Bitrián Carlota , Avis-Bodas Alicia , Porras Gracia , Borrego-Hernández Daniel , García-Redondo Alberto , Martín-Requero Angeles , Palomo Valle
Published: March 2021

CdSe quantum dots (QDs) are valuable tools for deciphering molecular mechanisms in cells. Their conjugation with antibodies offers a unique staining source with optimal characteristics, including increased photostability and narrow emission spectra, allowing for improved multiplexing capabilities using a single excitation source. In combination with pathology models derived from patients, they have great potential to contribute to quantitative molecular profiling and promote personalized medicine. However, the commercial availability of diverse CdSe QDs is still limited and characterization techniques must be performed to these materials or the conjugates developed in the lab to assure a proper function and reproducibility. Furthermore, while there is significant data of QDs experiments in cell lines, the literature with primary human cells is scarce, and QD behavior in these systems may be different. Rigorous characterization data of commercially available QDs and their conjugates with biomolecules of interest is needed in order to establish their potential for target labelling and expand their use among research labs. Here we compare the characterization and labelling performance of different QD conjugates in SH-SY5Y cell line, fibroblasts and immortalized lymphocytes derived from amyotrophic lateral sclerosis patients.
Fabrication and Characterization of High-Quality UV Photodetectors Based ZnO Nanorods Using Traditional and Modified Chemical Bath Deposition Methods
Authors: Abdulrahman Ahmed Fattah , Ahmed Sabah Mohammed , Barzinjy Azeez Abdullah , Hamad Samir Mustafa , Ahmed Naser Mahmoud , Almessiere Munirah Abullah
Published: March 2021

Ultraviolet (UV) photodetectors (PDs) based on high-quality well-aligned ZnO nanorods (NRs) were fabricated using both modified and conventional chemical bath deposition (CBD) methods. The modified chemical bath deposition (M-CBD) method was made by adding air bubbles to the growth solution during the CBD process. The viability and effectiveness of M-CBD were examined by developing UV PDs based on ZnO NRs. The ZnO nano-seed layer was coated on a glass substrate utilizing radiofrequency (RF) sputtering. The impact of the different growth-times on morphology, growth rate, crystal structure, and optical and chemical properties were investigated systematically using different characterization techniques, such as field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD) analysis, UV–VIS double beam spectrometer, and energy dispersive X-ray analysis (EDX), respectively. The Al/ZnO UV PDs based on ZnO nanorods were fabricated with optimum growth conditions through the two methods of preparation. This study showed that the synthesized ZnO NRs using the M-CBD method for different growth times possess better properties than the conventional method under similar deposition conditions. Despite having the highest aspect ratio and growth rate of ZnO NRs, which were found at 4 h growth duration for both methods, the aspect ratio of ZnO NRs using the M-CBD technique was comparatively higher than the conventional CBD method. Besides, the UV PDs fabricated by the M-CBD method at 5 V bias voltage showed high sensitivity, short response time, quick recovery time, high gain, low dark current, and high photocurrent compared with the UV PD device fabricated by the conventional CBD method.
Effective Removal of Crystal Violet Dye Using Neoteric Magnetic Nanostructures Based on Functionalized Poly(Benzofuran-co-Arylacetic Acid): Investigation of the Adsorption Behaviour and Reusability
Authors: Ganea Iolanda-Veronica , Nan Alexandrina , Baciu Călin , Turcu Rodica
Published: March 2021

Synthetic dyes represent a significant class of contaminants released in the environment. Crystal violet is a triarylmethane dye used in several fields such as printing inks, the textile or paper industries, as well as in cell histology. Coating magnetic nanoparticles with functionalized polymers has been proved to improve their efficiency, offering unique properties for applications in wastewater treatment. The current paper focuses on preparing and characterising magnetic core-shell nanoparticles coated with poly(benzofuran-co-arylacetic acid) functionalized with folic acid as an organic shell. The new polymer-based magnetic nanostructures were applied for crystal violet extraction from aqueous solutions. The nanostructures were structurally and morphologically investigated by Fourier-transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM). While thermal and magnetic properties of the magnetic nanostructures were determined by thermogravimetric analysis (TGA) and magnetization measurements (VSM). At the same time, crystal violet concentrations were determined by UV-VIS spectroscopy. The influence of initial dye concentration and contact time on the removal efficiency has been studied to achieve the optimum adsorption conditions. The dye adsorbent neoteric magnetic nanostructure was easily desorbed and reused, the adsorption capacity decreasing from 100% to 97.63% in the first five cycles, reaching a minimum of 88.74% after the 10th recycling step.
Orosensory Perception of Fat/Sweet Stimuli and Appetite-Regulating Peptides before and after Sleeve Gastrectomy or Gastric Bypass in Adult Women with Obesity
Authors: Bernard Arnaud , Le Beyec-Le Bihan Johanne , Radoi Loredana , Coupaye Muriel , Sami Ouidad , Casanova Nathalie , Le May Cédric , Collet Xavier , Delaby Pascaline , Le Bourgot Cindy , Besnard Philippe , Ledoux Séverine
Published: March 2021

The aim of this study was to explore the impact of bariatric surgery on fat and sweet taste perceptions and to determine the possible correlations with gut appetite-regulating peptides and subjective food sensations. Women suffering from severe obesity (BMI > 35 kg/m2) were studied 2 weeks before and 6 months after a vertical sleeve gastrectomy (VSG, n = 32) or a Roux-en-Y gastric bypass (RYGB, n = 12). Linoleic acid (LA) and sucrose perception thresholds were determined using the three-alternative forced-choice procedure, gut hormones were assayed before and after a test meal and subjective changes in oral food sensations were self-reported using a standardized questionnaire. Despite a global positive effect of both surgeries on the reported gustatory sensations, a change in the taste sensitivity was only found after RYGB for LA. However, the fat and sweet taste perceptions were not homogenous between patients who underwent the same surgery procedure, suggesting the existence of two subgroups: patients with and without taste improvement. These gustatory changes were not correlated to the surgery-mediated modifications of the main gut appetite-regulating hormones. Collectively these data highlight the complexity of relationships between bariatric surgery and taste sensitivity and suggest that VSG and RYGB might impact the fatty taste perception differently.
ROS Modulating Effects of Lingonberry (Vaccinium vitis-idaea L.) Polyphenols on Obese Adipocyte Hypertrophy and Vascular Endothelial Dysfunction
Authors: Kowalska Katarzyna , Dembczyński Radosław , Gołąbek Agata , Olkowicz Mariola , Olejnik Anna
Published: March 2021

Oxidative stress and dysregulated adipocytokine secretion accompanying hypertrophied adipose tissue induce chronic inflammation, which leads to vascular endothelial dysfunction. The present study investigated the ability of anthocyanin (ACN) and non-anthocyanin polyphenol (PP) fractions from lingonberry fruit to mitigate adipose tissue hypertrophy and endothelial dysfunction using 3T3-L1 adipocytes and human umbilical vein endothelial cells (HUVECs). This study showed that the PP fraction decreased intracellular ROS generation in hypertrophied adipocytes by enhancing antioxidant enzyme expression (SOD2) and inhibiting oxidant enzyme expression (NOX4, iNOS). Moreover, PP and ACN fractions reduced triglyceride content in adipocytes accompanied by downregulation of the expression of lipogenic genes such as aP2, FAS, and DAGT1. Treatment with both fractions modulated the mRNA expression and protein secretion of key adipokines in hypertrophied adipocytes. Expression and secretion of leptin and adiponectin were, respectively, down- and upregulated. Furthermore, PP and ACN fractions alleviated the inflammatory response in TNF-α-induced HUVECs by inhibiting the expression of pro-inflammatory genes (IL-6, IL-1β) and adhesion molecules (VCAM-1, ICAM-1, SELE). The obtained results suggest that consuming polyphenol-rich lingonberry fruit may help prevent and treat obesity and endothelial dysfunction due to their antioxidant and anti-inflammatory actions.
The Interplay between the Gut Microbiome and the Immune System in the Context of Infectious Diseases throughout Life and the Role of Nutrition in Optimizing Treatment Strategies
Authors: Wiertsema Selma P. , van Bergenhenegouwen Jeroen , Garssen Johan , Knippels Leon M. J.
Published: March 2021

Infectious diseases and infections remain a leading cause of death in low-income countries and a major risk to vulnerable groups, such as infants and the elderly. The immune system plays a crucial role in the susceptibility, persistence, and clearance of these infections. With 70–80% of immune cells being present in the gut, there is an intricate interplay between the intestinal microbiota, the intestinal epithelial layer, and the local mucosal immune system. In addition to the local mucosal immune responses in the gut, it is increasingly recognized that the gut microbiome also affects systemic immunity. Clinicians are more and more using the increased knowledge about these complex interactions between the immune system, the gut microbiome, and human pathogens. The now well-recognized impact of nutrition on the composition of the gut microbiota and the immune system elucidates the role nutrition can play in improving health. This review describes the mechanisms involved in maintaining the intricate balance between the microbiota, gut health, the local immune response, and systemic immunity, linking this to infectious diseases throughout life, and highlights the impact of nutrition in infectious disease prevention and treatment.
The Role of Galcanezumab in Migraine Prevention: Existing Data and Future Directions
Authors: Gklinos Panagiotis , Mitsikostas Dimos
Published: March 2021

Galcanezumab is a humanized monoclonal antibody blocking the calcitonin gene-related peptide (CGRP) pathway by targeting the CGRP. Data from four phase-3 randomized placebo-controlled clinical trials showed that galcanezumab is superior to placebo in reducing migraine headaches, migraine-specific quality of life, and headache-related disability. Most of the adverse events (AEs) were mild to moderate and did not affect trial completion rates significantly. Along with erenumab, fremanezumab, and eptinezumab, galcanezumab forms a novel class of anti-migraine preventative treatments that is disease-specific and mechanism-based, unlike the standard ones. In addition, galcanezumab has also been shown to be effective in cluster headache, though more clinical trials are required. Overall, galcanezumab is a promising emerging treatment in migraine prophylaxis. However, it needs to be tested in larger clinical trials focused on treatment-resistant migraine. Furthermore, its safety profile, especially its potential association with an increased cardiovascular risk, needs to be established through long-term, real-world data. This review aims to give an overview of its pharmacological properties as well as to report and discuss data from clinical trials and its potential place in headache therapeutics.
Dual Anti-Malarial and GSK3β-Mediated Cytokine-Modulating Activities of Quercetin Are Requisite of Its Potential as a Plant-Derived Therapeutic in Malaria
Authors: Ali Amatul Hamizah , Sudi Suhaini , Shi-Jing Ng , Hassan Wan Rozianoor Mohd , Basir Rusliza , Agustar Hani Kartini , Embi Noor , Sidek Hasidah Mohd , Latip Jalifah
Published: March 2021

Although death in malaria is attributed to cerebrovascular blockage and anaemia, overwhelming cytokine production can contribute to the severity of the disease. Therefore, mitigation of dysregulated inflammatory signalling may provide further benefit for malaria treatment. Quercetin (3,3′,4′,5,7-pentahydroxyflavone) is known to inhibit glycogen synthase kinase-3β (GSK3β), a potent regulator of both pro- and anti-inflammatory effects. Quercetin is therefore a potential therapeutic to modulate the imbalanced cytokine production during malarial infection. Anti-malarial effects of quercetin were evaluated in murine models of severe and cerebral malaria using Plasmodium berghei NK65 and ANKA strains, respectively. Western blotting and analysis of cytokines were carried out to determine the GSK3β-mediated cytokine-modulating effects of quercetin in infected animals. Quercetin (25 mg/kg BW) treatment in P. berghei NK65-infected animals resulted in 60.7 ± 2.4% suppression of parasitaemia and significantly decreased serum levels of TNF-α and IFN-γ, whilst levels of IL-10 and IL-4 were elevated significantly. Western analysis revealed that pGSK3β (Ser9) increased 2.7-fold in the liver of quercetin-treated NK65-infected animals. Treatment of P. berghei ANKA-infected mice with quercetin (15 mg/kg BW) increased (2.3-fold) pGSK3β (Ser9) in the brains of infected animals. Quercetin is a potential plant-derived therapeutic for malaria on the basis that it can elicit anti-malarial and GSK3β-mediated cytokine-modulating effects.
A Novel Isolate of Bacillus cereus Promotes Growth in Tomato and Inhibits Clavibacter michiganensis Infection under Greenhouse Conditions
Authors: Solano-Alvarez Nallely , Valencia-Hernández Juan Antonio , Rico-García Enrique , Torres-Pacheco Irineo , Ocampo-Velázquez Rosalía Virginia , Escamilla-Silva Eleazar Máximo , Romero-García Ana Luz , Alpuche-Solís Ángel G. , Guevara-González Ramón Gerardo
Published: March 2021

The need to produce food in a sustainable way to counteract the effects of excessive use of agrochemicals opens the door to the generation of new technologies that are not based on fossil fuels and are less toxic to ecosystems. Plant growth-promoting bacteria (PGPB) could represent an alternative to chemical biofertilizers and pesticides offering protection for biotic and abiotic stresses. In this work, a bacterial isolate from roots of castor bean (Ricinus communis) was identified and named as Bacillus cereus strain “Amazcala” (B.c-A). This isolate displayed the ability to solubilize inorganic phosphate and produce gibberellic acid (GA3). Moreover, this bacterium provided significant increases in height, stem width, dry weight, and total chlorophyll content in tomato plants. Interestingly, B.c-A also significantly decreased the severity of bacterial canker disease on tomato caused by Clavibacter michiganensis (Cmm) in preventive disease assays under greenhouse conditions. Based on our results, B.c-A can be considered as PGPB and a useful tool in Cmm disease control on tomato plant under greenhouse conditions.
Evolutionary and Ecological Considerations on Nectar-Mediated Tripartite Interactions in Angiosperms and Their Relevance in the Mediterranean Basin
Authors: Nepi Massimo , Calabrese Daniele , Guarnieri Massimo , Giordano Emanuele
Published: March 2021

The Mediterranean basin hosts a high diversity of plants and bees, and it is considered one of the world’s biodiversity hotspots. Insect pollination, i.e., pollen transfer from male reproductive structures to conspecific female ones, was classically thought to be a mutualistic relationship that links these two groups of organisms, giving rise to an admirable and complex network of interactions. Although nectar is often involved in mediating these interactions, relatively little is known about modifications in its chemical traits during the evolution of plants. Here, we examine how the current sucrose-dominated floral nectar of most Mediterranean plants could have arisen in the course of evolution of angiosperms. The transition from hexose-rich to sucrose-rich nectar secretion was probably triggered by increasing temperature and aridity during the Cretaceous period, when most angiosperms were radiating. This transition may have opened new ecological niches for new groups of insects that were co-diversifying with angiosperms and for specific nectar-dwelling yeasts that originated later (i.e., Metschnikowiaceae). Our hypothesis embeds recent discoveries in nectar biology, such as the involvement of nectar microbiota and nectar secondary metabolites in shaping interactions with pollinators, and it suggests a complex, multifaceted ecological and evolutionary scenario that we are just beginning to discover.
Genotyping-by-Sequencing in Vigna unguiculata Landraces and Its Utility for Assessing Taxonomic Relationships
Authors: Zuluaga Diana Lucia , Lioi Lucia , Delvento Chiara , Pavan Stefano , Sonnante Gabriella
Published: March 2021

Genotyping by sequencing (GBS) was used to analyze relationships among cowpea and asparagus bean landraces from southern Italy and to assess the utility of this technology to study taxonomy in a wider panel, including V. unguiculata cultigroups, subspecies, and other Vigna species. The analysis of SNPs derived from GBS highlighted that, among the cowpea landraces, the African samples were separated from the other material, while, for the Italian landraces, a certain clustering depending on seed color/pattern was observed in the dendrogram. When examining the V. unguiculata species complex, a clear separation between the two groups of wild subspecies, i.e., the allogamous wild perennials and the perennial out/inbreds, could be observed, the former representing the more ancestral wild progenitors of V. unguiculata. The species V. vexillata appeared more closely related to V. unguiculata than to the other Vigna species analyzed.
Micromorphology, Ultrastructure and Histochemistry of Commelina benghalensis L. Leaves and Stems
Authors: Doolabh Kareshma , Naidoo Yougasphree , Dewir Yaser Hassan , Al-Suhaibani Nasser
Published: March 2021

Commelina benghalensis L. is used as a traditional medicine in treating numerous ailments and diseases such as infertility in women, conjunctivitis, gonorrhea, and jaundice. This study used light and electron microscopy coupled with histochemistry to investigate the micromorphology, ultrastructure and histochemical properties of C. benghalensis leaves and stems. Stereo and scanning electron microscopy revealed dense non-glandular trichomes on the leaves and stems and trichome density was greater in emergent leaves than in the young and mature. Three morphologically different non-glandular trichomes were observed including simple multicellular, simple bicellular and simple multicellular hooked. The simple bicellular trichomes were less common than the multicellular and hooked. Transmission electron micrographs showed mitochondria, vesicles and vacuoles in the trichome. The leaf section contained chloroplasts with plastoglobuli and starch grains. Histochemical analysis revealed various pharmacologically important compounds such as phenols, alkaloids, proteins and polysaccharides. The micromorphological and ultrastructural investigations suggest that Commelina benghalensis L. is an economically important medicinal plant due to bioactive compounds present in the leaves and stems.
Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves
Authors: Zhai Zhiyang , Liu Hui , Shanklin John
Published: March 2021

During the transformation of wild-type (WT) Arabidopsis thaliana, a T-DNA containing OLEOSIN-GFP (OLE1-GFP) was inserted by happenstance within the GBSS1 gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining gbss1 with the expression of OLE1-GFP was confirmed by transforming an independent gbss1 mutant (GABI_914G01) with OLE1-GFP. The resulting OLE1-GFP/gbss1 transgenic lines showed higher leaf oil content than the individual OLE1-GFP/WT or single gbss1 mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in OG significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties.
Effect of Crosslinking Type on the Physical-Chemical Properties and Biocompatibility of Chitosan-Based Electrospun Membranes
Authors: Dodero Andrea , Scarfi Sonia , Mirata Serena , Sionkowska Alina , Vicini Silvia , Alloisio Marina , Castellano Maila
Published: March 2021

Chitosan nanofibrous membranes are prepared via an electrospinning technique and explored as potential wound healing patches. In particular, the effect of a physical or chemical crosslinking treatment on the mat morphological, mechanical, water-related, and biological properties is deeply evaluated. The use of phosphate ions (i.e., physical crosslinking) allows us to obtain smooth and highly homogenous nanofibers with an average size of 190 nm, whereas the use of ethylene glycol diglycidyl ether (i.e., chemical crosslinking) leads to rougher, partially coalesced, and bigger nanofibers with an average dimension of 270 nm. Additionally, the physically crosslinked mats show enhanced mechanical performances, as well as greater water vapour permeability and hydrophilicity, with respect to the chemically crosslinked ones. Above all, cell adhesion and cytotoxicity experiments demonstrate that the use of phosphate ions as crosslinkers significantly improves the capability of chitosan mats to promote cell viability owing to their higher biocompatibility. Moreover, tuneable drug delivery properties are achieved for the physically crosslinked mats by a simple post-processing impregnation methodology, thereby indicating the possibility to enrich the prepared membranes with unique features. The results prove that the proposed approach may lead to the preparation of cheap, biocompatible, and efficient chitosan-based nanofibers for biomedical and pharmaceutical applications.
A Win–Win Combination to Inhibit Persistent Organic Pollutant Formation via the Co-Incineration of Polyvinyl Chloride E-Waste and Sewage Sludge
Authors: Gandon-Ros Gerard , Nuñez Samuel S. , Ortuño Nuria , Aracil Ignacio , Gómez-Rico María Francisca , Conesa Juan A.
Published: March 2021

Persistent organic pollutant inhibition in the combustion process of polyvinyl chloride (PVC) by prior addition of an inhibitor is currently being studied, reducing the emission of pollutants, and thus reducing the large amount of waste PVC destined for landfill. In this work, the use of sewage sludge (SS) as an alternative to chemical inhibitors to improve the quality emissions of the incineration of polyvinyl chloride waste (PVC e-waste) was studied and optimized. Different combustion runs were carried out at 850 °C in a laboratory tubular reactor, varying both the molar ratio Ri (0.25, 0.50, 0.75) between inhibitors (N + S) and chlorine (Cl) and the oxygen ratio λ (0.15, 0.50) between actual oxygen and stoichiometric oxygen. The emissions of several semivolatile compounds families such as polycyclic aromatic hydrocarbons (PAHs), polychlorobenzenes (ClBzs), and polychlorophenols (ClPhs), with special interest in the emissions of the most toxic compounds, i.e., polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dl-PCBs), were analyzed. A notable decrease in PCDD/F and dl-PCB formation was achieved in most of the experiments, especially for those runs performed under an oxygen-rich atmosphere (λ = 0.50), where the addition of sludge was beneficial with inhibition ratios Ri ≥ 0.25. An inhibition ratio of 0.75 showed the best results with almost a 100% reduction in PCDD/F formation and a 95% reduction in dl-PCB formation.
Glucose-Assisted One-Pot Hydrothermal Synthesis of Hierarchical-Structured MoS2/C Quasi-Hollow Microspheres for High-Performance Lithium Ion Battery
Authors: Liu Xingang , Tan Jiang , Li Xi , Zhang Chuhong
Published: March 2021

In this work, hierarchical MoS2/C quasi-hollow microspheres are prepared by a one-pot hydrothermal process with the addition of glucose. The glucose is not only inclined to form the roundish sphere in the completion of the synthesis of MoS2, but at the same time the microspheres formed by the glucose can act as the nuclei on which the MoS2 grows. Glucose, acting as a nucleating agent, has the advantages of being low-cost and environmentally friendly, which can simplify the fabrication process. The interiors of the MoS2/C samples are multi-hole and quasi-hollow, which is beneficial for the insertion and extraction of lithium ions. For the first time, we demonstrate that hierarchical-structured MoS2/C quasi-hollow microspheres exhibit an excellent cycling stability and rate capability in lithium ion batteries (LIBs) and are significantly superior to the bulk MoS2. The method presented in this article may provide a simple, clean. and economical strategy for the preparation of MoS2/C microspheres as a feasible and promising anode material for LIBs.
A New Cycle-Slip Repair Method for Dual-Frequency BDS Against the Disturbances of Severe Ionospheric Variations and Pseudoranges with Large Errors
Authors: Li Dehai , Dang Yaming , Yuan Yunbin , Mi Jinzhong
Published: March 2021

Many Beidou navigation satellite system (BDS) receivers or boards provide dual-frequency measurements to conduct precise positioning and navigation for low-power consumption. Cycle-slip processing is a primary work to guarantee consistent, precise positioning with the phase data. However, the cycle-slip processing of BDS dual-frequency phases still follows with those of existing GPS methods. For single-satellite data, cycle-slip detection (CSD) with the geometry-free phase (GF) is disturbed by severe ionospheric delay variations, while CSD or cycle-slip repair (CSR) with the Melbourne–Wubbena combination (MW) must face the risk of the tremendous disturbance from large pseudorange errors. To overcome the above limitations, a new cycle-slip repair method for BDS dual-frequency phases (BDCSR) is proposed: (1) An optimal model to minimize the variance of the cycle-slip calculation was established to the dual-frequency BDS, after correcting the ionospheric variation with a reasonable and feasible way. (2) Under the BDS dual-frequency condition, a discrimination function was built to exclude the adverse disturbance from the pseudorange errors on the CSR, according to the rankings of the absolute epoch-difference GFs calculated by the searched cycle-slip candidates after correcting the ionospheric variation. Subsequently, many compared CSR tests were implemented in conditions of low and medium elevations during strong geomagnetic storms. Comparisons from the results of different methods show that: (1) The variations of ionospheric delays are intolerable in the cycle-slip calculation during the geomagnetic storm, and the tremendous influence from the ionospheric variation should be corrected before calculating the cycle-slip combination with the BDS dual-frequency data. (2) Under the condition of real dual-frequency BDS data during the geomagnetic storm, the actual success rate of the conventional dual-frequency CSR (CDCSR) by employing the optimized combinations, but absenting from the discrimination function, is lower than that of BDCSR by about 2%; The actual success rate of the CSD with MW (MWCSD), is lower than that of BDCSR by about 2%. (3) After adding gross errors of 0.7 m to all real epoch-difference pseudoranges epoch-by-epoch, results of CDCSR and MWCSD showed many errors. However, BDCSR achieved a higher actual success rate than those of CDCSR and MWCSD, about 43% and 16%, respectively, and better performance of refraining the disturbance of large pseudorange error on the cycle-slip determination was achieved in the BDCSR methodology.
Advanced Dual-Satellite Method for Detection of Low Stratus and Fog near Japan at Dawn from FY-4A and Himawari-8
Authors: Yang Jung-Hyun , Yoo Jung-Moon , Choi Yong-Sang
Published: March 2021

The detection of low stratus and fog (LSF) at dawn remains limited because of their optical features and weak solar radiation. LSF could be better identified by simultaneous observations of two geostationary satellites from different viewing angles. The present study developed an advanced dual-satellite method (DSM) using FY-4A and Himawari-8 for LSF detection at dawn in terms of probability indices. Optimal thresholds for identifying the LSF from the spectral tests in DSM were determined by the comparison with ground observations of fog and clear sky in/around Japan between April to November of 2018. Then the validation of these thresholds was carried out for the same months of 2019. The DSM essentially used two traditional single-satellite tests for daytime such as the 0.65-μm reflectance (R0.65), and the brightness temperature difference between 3.7 μm and 11 μm (BTD3.7-11); in addition to four more tests such as Himawari-8 R0.65 and BTD13.5-8.5, the dual-satellite stereoscopic difference in BTD3.7-11 (ΔBTD3.7-11), and that in the Normalized Difference Snow Index (ΔNDSI). The four were found to show very high skill scores (POD: 0.82 ± 0.04; FAR, 0.10 ± 0.04). The radiative transfer simulation supported optical characteristics of LSF in observations. The LSF probability indices (average POD: 0.83, FAR: 0.10) were constructed by a statistical combination of the four to derive the five-class probability values of LSF occurrence in a grid. The indices provided more details and useful results in LSF spatial distribution, compared to the single satellite observations (i.e., R0.65 and/or BTD3.7-11) of either LSF or no LSF. The present DSM could apply for remote sensing of environmental phenomena if the stereoscopic viewing angle between two satellites is appropriate.
Metal Oxide Nanorods-Based Sensor Array for Selective Detection of Biomarker Gases
Authors: Kim Gwang Su , Park Yumin , Shin Joonchul , Song Young Geun , Kang Chong-Yun
Published: March 2021

The breath gas analysis through gas phase chemical analysis draws attention in terms of non-invasive and real time monitoring. The array-type sensors are one of the diagnostic methods with high sensitivity and selectivity towards the target gases. Herein, we presented a 2 × 4 sensor array with a micro-heater and ceramic chip. The device is designed in a small size for portability, including the internal eight-channel sensor array. In2O3 NRs and WO3 NRs manufactured through the E-beam evaporator’s glancing angle method were used as sensing materials. Pt, Pd, and Au metal catalysts were decorated for each channel to enhance functionality. The sensor array was measured for the exhaled gas biomarkers CH3COCH3, NO2, and H2S to confirm the respiratory diagnostic performance. Through this operation, the theoretical detection limit was calculated as 1.48 ppb for CH3COCH3, 1.9 ppt for NO2, and 2.47 ppb for H2S. This excellent detection performance indicates that our sensor array detected the CH3COCH3, NO2, and H2S as biomarkers, applying to the breath gas analysis. Our results showed the high potential of the gas sensor array as a non-invasive diagnostic tool that enables real-time monitoring.
Sustainable Conversion of Renewable Energy Sources
Authors: Sher Farooq , Curnick Oliver , Azizan Mohammad Tazli
Published: March 2021

Global energy requirements are rising tremendously because of increasing urbanization and the human population. In the last few centuries, the consumption of fossil fuels has caused increased emissions of greenhouse gases resulting in environmental concerns like global warming, climatic change, and biodiversity loss. As a result, progress in sustainable energy has become the centre of attention in climatic change agenda and economic growth. Until now, various methods of renewable energy production have been extensively studied such as geothermal energy, wind energy, and solar energy. In addition to this, the utilization of biofuels from different sustainable sources are also being considered to reduce greenhouses gas emissions. New approaches and developments are still required for the creation of more sustainable, efficient, and affordable renewable energy systems and for the mitigation of global environmental threats. This special issue aims to advance novel developments in the sustainable conversion of renewable energy, providing up to date, fruitful, and actionable insights into economic, social, and environmental sustainability and includes original research articles and reviews to describe the interaction between renewable fuels, CO2 mitigation, and global warming.
Monitoring Land Use/Cover Change Using Remotely Sensed Data in Guangzhou of China
Authors: Guo Liang , Xi Xiaohuan , Yang Weijun , Liang Lei
Published: March 2021

Land use/cover change (LUCC) has a crucial influence on ecosystem function, environmental change and decision support. Rapid and precise monitoring of land use/cover change information is essential for utilization and management of land resources. The objectives of this study were to monitor land use/cover change of Guangzhou of China from 1986 to 2018 using remotely sensed data, and analyze the correlation between artificial surface expansion and the gross domestic product (GDP) growth. Supervised classification was performed using Random Forest classifier, and the overall accuracy (OA) ranged from 86.42% to 96.58% and kappa coefficient (K) ranged from 0.8079 to 0.9499. The results show that the built-up area of Guangzhou of China from 1986 to 2018 continued to increase. However, the vegetation area continued to decrease during 32 years. The built-up area increased by 1315.56 km2 (increased by 439.34%) with an average growth of 41.11 km2/year. The vegetation area reduced by 1290.78 km2 (reduced by 19.99%) with an average reduction of 40.34 km2/year. Research has shown that the reduced vegetation area was mainly converted into built-up area. The area of water bodies and bare lands was relatively stable and had a little change. The results indicate that the GDP had a strong positive correlation with built-up area (R2 = 0.98). However, there is a strong negative correlation between the GDP and vegetation area (R2 = 0.97) in Guangzhou City, China. As a consequence, the increase of built-up area was at the cost of the reduction of vegetation area.
Structural Model to Determine the Factors That Affect the Quality of Emergency Teaching, According to the Perception of the Student of the First University Courses
Authors: del Arco Isabel , Flores Òscar , Ramos-Pla Anabel
Published: March 2021

A quantitative study was conducted in order to know, from the perspective of university students, the relationship between the quality perceived (QP) during the period of confinement derived from the SARS-CoV-2 virus, with the variables teaching plan (PL), material resources (MR), interaction processes (IN), and the affective–emotional component (EM). An online questionnaire was designed, directed to students from 20 universities in Spain, with a total participation of 893 individuals. The results indicate that the perception of the students on the quality of online teaching is directly associated with the material resources provided by the professors and the professor–student interactions. However, this perception does not have any direct effect on the planning or the emotional state or affectation created by the unprecedented situation of confinement. Among the conclusions, we highlight the need for the universities to apply models of support and tutoring, especially for students in their first years at university, to develop competences such as autonomy, digital competence, and self-regulation, and the need for a change of approach of the students and the professors based on the new normality we are currently experiencing.
Knowledge Management Practices for Sustainable Supply Chain Management: A Challenge for Business Education
Authors: Kassaneh Tomas Cherkos , Bolisani Ettore , Cegarra-Navarro Juan-Gabriel
Published: March 2021

In the last decades, business competition has been increasingly among supply chains (SCs) rather than individual firms. Today, considering the challenges of environmental, social, and economic sustainability, it is becoming even more vital to coordinate and co-manage company resources, activities, and innovative efforts at the SC level. Consequently, knowledge, which is a critical resource for companies, needs to be managed properly not only in single firms but also across SCs. For the education of business managers, this implies a double challenge: first, to make students and future executives become aware of the knowledge management (KM) practices that can be adopted; second, to facilitate the assimilation of these practices for the effective management of SCs, to ensure higher economic and environmentally sustainable performances. Standard definitions and classifications can be of great help, but the current studies are very fragmented. This study contributes by exploring the literature and examining the KM practices that are proposed and defined by the different authors. A systematic review and a descriptive analysis of selected papers showed the trend and focus of papers in the KM and SC fields. In addition, based on the definitions and classifications drawn from the literature, this paper discusses a possible systematization of the key KM practices in SCs. The major contribution of this paper is the effort of re-definition and re-classification of KM practices and their potential importance for effective and sustainable SC management. This analysis can be especially useful for organizing KM courses targeted to current and future business managers.