bluuebunny/crossref_metadata_2025 huggingface dataset:
Win-wins or trade-offs? Site and strategy determine carbon and local ecosystem service benefits for protection, restoration, and agroforestry
Authors: McDonald Robert I. , Chaplin-Kramer Rebecca , Mulligan Mark , Kropf Chahan M. , Hülsen Sarah , Welker Preston , Poor Erin , Erbaugh James T. , Masuda Yuta J.
Published: August 2024

Nature-based solutions (NBS) can deliver many benefits to human wellbeing, including some crucial to climate adaptation. We quantitatively assess the global potential of NBS strategies of protection, restoration, and agroforestry by modeling global climate change mitigation and local ecosystem services (water availability, sediment retention, runoff, pollination, nitrogen retention, green water storage, and coastal protection). The strategies with the most potential to help people do not necessarily deliver the most climate change mitigation: per area of conservation action, agroforestry provides substantial benefits (>20% increase in at least one local ecosystem service) to three times more people on average than reforestation while providing less than one tenth the carbon sequestration per unit area. Each strategy delivers a different suite of ecosystem service benefits; for instance, avoided forest conversion provides a strong increase in nitrogen retention (100% increase to 72 million people if fully implemented globally) while agroforestry increases pollination services (100% increase to 3.0 billion people if fully implemented globally). One common disservice shared by all the NBS strategies modeled here is that increased woody biomass increases transpiration, reducing annual runoff and in some watersheds negatively impacting local water availability. In addition, the places with the greatest potential for climate change mitigation are not necessarily the ones with the most people. For instance, reforestation in Latin America has the greatest climate change mitigation potential, but the greatest ecosystem service benefits are in Africa. Focusing on nations with high climate mitigation potential as well as high local ecosystem service potential, such as Nigeria in the case of reforestation, India for agroforestry, and the Republic of Congo for avoided forest conversion, can help identify win-win sites for implementation. We find that concentrating implementation of these three conservation strategies in critical places, covering 5.8 million km2, could benefit 2.0 billion people with increased local ecosystem services provision. These critical places cover only 35% of the possible area of implementation but would provide 80% of the benefits that are possible globally for the selected set of ecosystem services under the NBS scenarios examined here. We conclude that targeting these critical places for protection, restoration, and agroforestry interventions will be key to achieving adaptation and human wellbeing goals while also increasing nature-based carbon mitigation.
Developing an SSA-optimized attention-ConvGRU model for predicting and assessing soil contaminant distribution
Authors: Liang Yajie , Zhao Jieyu , Zhang Yiting , Li Jisheng , Ding Jieran , Jing Changyong , Ji Jiukun , Wu Dongtan
Published: August 2024

Introduction: Soil pollution, which includes a variety of contaminants such as heavy metals and organic compounds, poses significant environmental and health risks, making effective prediction and assessment techniques essential. Current predictive models often struggle with the complexity and diversity of soil contaminant behaviors, leading to limitations in their accuracy and applicability.

Methods: Recognizing the importance of capturing the temporal dynamics influenced by seasonal variations and agricultural practices, our study introduces an SSA-optimized Attention-ConvGRU model. This model integrates convolutional neural networks, gated recurrent units, and attention mechanisms, enhanced through optimization with the Sparrow Search Algorithm to improve predictive performance.

Results: Experimental results confirm that our model significantly outperforms traditional methods, demonstrating over 30% improvement in prediction accuracy across multiple datasets.

Discussion: This research underscores the potential of advanced machine learning techniques to revolutionize the assessment of soil pollution, providing substantial benefits for environmental management and public health protection.
The role of primary cilia in congenital heart defect-associated neurological impairments
Authors: Sarić Nemanja , Ishibashi Nobuyuki
Published: August 2024

Congenital heart disease (CHD) has, despite significant improvements in patient survival, increasingly become associated with neurological deficits during infancy that persist into adulthood. These impairments afflict a wide range of behavioral domains including executive function, motor learning and coordination, social interaction, and language acquisition, reflecting alterations in multiple brain areas. In the past few decades, it has become clear that CHD is highly genetically heterogeneous, with large chromosomal aneuploidies and copy number variants (CNVs) as well as single nucleotide polymorphisms (SNPs) being implicated in CHD pathogenesis. Intriguingly, many of the identified loss-of-function genetic variants occur in genes important for primary cilia integrity and function, hinting at a key role for primary cilia in CHD. Here we review the current evidence for CHD primary cilia associated genetic variants, their independent functions during cardiac and brain development and their influence on behavior. We also highlight the role of environmental exposures in CHD, including stressors such as surgical factors and anesthesia, and how they might interact with ciliary genetic predispositions to determine the final neurodevelopmental outcome. The multifactorial nature of CHD and neurological impairments linked with it will, on one hand, likely necessitate therapeutic targeting of molecular pathways and neurobehavioral deficits shared by disparate forms of CHD. On the other hand, strategies for better CHD patient stratification based on genomic data, gestational and surgical history, and CHD complexity would allow for more precise therapeutic targeting of comorbid neurological deficits.
Cannabinoids and triple-negative breast cancer treatment
Authors: Dobovišek Luka , Borštnar Simona , Debeljak Nataša , Kranjc Brezar Simona
Published: August 2024

Triple-negative breast cancer (TNBC) accounts for about 10-20% of all breast cancer cases and is associated with an unfavorable prognosis. Until recently, treatment options for TNBC were limited to chemotherapy. A new successful systemic treatment is immunotherapy with immune checkpoint inhibitors, but new tumor-specific biomarkers are needed to improve patient outcomes. Cannabinoids show antitumor activity in most preclinical studies in TNBC models and do not appear to have adverse effects on chemotherapy. Clinical data are needed to evaluate efficacy and safety in humans. Importantly, the endocannabinoid system is linked to the immune system and immunosuppression. Therefore, cannabinoid receptors could be a potential biomarker for immune checkpoint inhibitor therapy or a novel mechanism to reverse resistance to immunotherapy. In this article, we provide an overview of the currently available information on how cannabinoids may influence standard therapy in TNBC.
In pancreatic cancer patients, chemotherapy reshapes the gene expression profile and antigen receptor repertoire of T lymphocytes and enhances their effector response to tumor-associated antigens
Authors: Brugiapaglia Silvia , Bulfamante Sara , Curcio Claudia , Arigoni Maddalena , Calogero Raffaele , Bonello Lisa , Genuardi Elisa , Spadi Rosella , Satolli Maria Antonietta , Campra Donata , Giordano Daniele , Cappello Paola , Cordero Francesca , Novelli Francesco
Published: August 2024

Introduction

Pancreatic Ductal Adenocarcinoma (PDA) is one of the most aggressive malignancies with a 5-year survival rate of 13%. Less than 20% of patients have a resectable tumor at diagnosis due to the lack of distinctive symptoms and reliable biomarkers. PDA is resistant to chemotherapy (CT) and understanding how to gain an anti-tumor effector response following stimulation is, therefore, critical for setting up an effective immunotherapy.

Methods

Proliferation, and cytokine release and TCRB repertoire of from PDA patient peripheral T lymphocytes, before and after CT, were analyzed in vitro in response to four tumor-associated antigens (TAA), namely ENO1, FUBP1, GAPDH and K2C8. Transcriptional state of PDA patient PBMC was investigated using RNA-Seq before and after CT.

Results

CT increased the number of TAA recognized by T lymphocytes, which positively correlated with patient survival, and high IFN-γ production TAA-induced responses were significantly increased after CT. We found that some ENO1-stimulated T cell clonotypes from CT-treated patients were expanded or de-novo induced, and that some clonotypes were reduced or even disappeared after CT. Patients that showed a higher number of effector responses to TAA (high IFN-γ/IL-10 ratio) after CT expressed increased fatty acid-related transcriptional signature. Conversely, patients that showed a higher number of regulatory responses to TAA (low IFN-γ/IL-10 ratio) after CT significantly expressed an increased IRAK1/IL1R axis-related transcriptional signature.

Conclusion

These data suggest that the expression of fatty acid or IRAK1/IL1Rrelated genes predicts T lymphocyte effector or regulatory responses to TAA in patients that undergo CT. These findings are a springboard to set up precision immunotherapies in PDA based on the TAA vaccination in combination with CT.
Multi-omics pan-cancer analysis reveals the prognostic values and immunological functions of PPA2, with a spotlight on breast cancer
Authors: Zhang Jia-Ning , Yang Bei-Bei , Li Lin-Wei , Xu Hao , Wang Bin , Yi Zi-Lu , Zhou Xi-Rui , Liu Hong
Published: August 2024

Background

Recently, the role of inorganic pyrophosphatase 2 (PPA2) has been remaining merely superficial in many tumors. Hence, the aim was to analyze the potential functions of PPA2 in pan-cancer, focusing on its role in breast cancer.

Methods

A systematic pan-cancer analysis conducted primarily utilizing various open databases such as TCGA and GTEx. We explored the clinical value of PPA2 as well as various biological functions, including expression levels and subcellular localization, multi-dimensional immune-correlation analysis, co-expression networks, and gene heterogeneity. In addition, we not only verified the function of PPA2 through cell experiments but also analyzed PPA2 at the single-cell level and its drug sensitivity.

Results

PPA2 is abnormally expressed in various tumors, and it is mainly distributed in mitochondria. Furthermore, the indicators (OS, DSS, DFI, and PFI) of analysis hint that PPA2 exhibits significant prognostic value. At the same time, the genomic heterogeneity (including TMB, MSI, MATH, and NEO) of PPA2 in pan-cancer was analyzed. Across multiple tumors, the results showed a close correlation between PPA2 expression levels and different immune signatures (such as immune cell infiltration). All of these indicate that PPA2 could potentially be applied in the guidance of immunotherapy. We also have demonstrated that PPA2 promoted the process of breast cancer. Finally, some potential therapeutic agents (such as Fulvestrant) targeting the abnormal expression of PPA2 are revealed.

Conclusion

In conclusion, the results demonstrated the great value of PPA2 in pan-cancer research, as well as its potential as a therapeutic target for breast tumors.
Less is more! Low amount of Fusobacterium nucleatum supports macrophage-mediated trophoblast functions in vitro
Authors: Einenkel Rebekka , Ehrhardt Jens , Zygmunt Marek , Muzzio Damián Oscar
Published: August 2024

F. nucleatum, involved in carcinogenesis of colon carcinomas, has been described as part of the commensal flora of the female upper reproductive tract. Although its contribution to destructive inflammatory processes is well described, its role as commensal uterine bacteria has not been thoroughly investigated. Since carcinogenesis shares similar mechanisms with early pregnancy development (including proliferation, invasion, blood supply and the induction of tolerance), these mechanisms induced by F. nucleatum could play a role in early pregnancy. Additionally, implantation and placentation require a well-balanced immune activation, which might be suitably managed by the presence of a limited amount of bacteria or bacterial residues. We assessed the effect of inactivated F. nucleatum on macrophage-trophoblast interactions. Monocytic cells (THP-1) were polarized into M1, M2a or M2c macrophages by IFN-γ, IL-4 or TGF-β, respectively, and subsequently treated with inactivated fusobacteria (bacteria:macrophage ratio of 0.1 and 1). Direct effects on macrophages were assessed by viability assay, flow cytometry (antigen presentation molecules and cytokines), qPCR (cytokine expression), in-cell Western (HIF and P-NF-κB) and ELISA (VEGF secretion). The function of first trimester extravillous trophoblast cells (HTR-8/SVneo) in response to macrophage-conditioned medium was microscopically assessed by migration (scratch assay), invasion (sprouting assay) and tube formation. Underlying molecular changes were investigated by ELISA (VEGF secretion) and qPCR (matrix-degrading factors and regulators). Inflammation-primed macrophages (M1) as well as high bacterial amounts increased pro-inflammatory NF-κB expression and inflammatory responses. Subsequently, trophoblast functions were impaired. In contrast, low bacterial stimulation caused an increased HIF activation and subsequent VEGF-A secretion in M2c macrophages. Accordingly, there was an increase of trophoblast tube formation. Our results suggest that a low-mass endometrial/decidual microbiome can be tolerated and while it supports implantation and further pregnancy processes.
The MET Norway Ice Service: a comprehensive review of the historical and future evolution, ice chart creation, and end user interaction within METAREA XIX
Authors: Copeland William , Wagner Penelope , Hughes Nick , Everett Alistair , Robertsen Trond
Published: August 2024

The MET Norway Ice Service (NIS) celebrated its fiftieth year as a formal operational sea ice information provider in 2020. Prior to the 1970’s, support to navigation had started off with ad-hoc observations from coastal stations on Svalbard in the 1930’s, before developing as a research programme in the 1960’s. Activity in the region has steadily increased, and now the NIS also supports a large number of research, tourist, and resource exploration vessels, in addition to the ice chart archive being a resource for climate change research. The Ice Service has always been at the forefront in the use of satellite Earth Observation technologies, beginning with the routine use of optical thermal infrared imagery from NASA TIROS and becoming a large user of Canadian RADARSAT-2 Synthetic Aperture Radar (SAR), and then European Copernicus Sentinel-1, in the 2000’s and 2010’s. Initially ice charts were a weekly compilation of ice information using cloud-free satellite coverage, aerial reconnaissance, and in situ observations, drawn on paper at the offices of the Norwegian Meteorological Institute (MET Norway) in Oslo. From 1997 production moved to the Tromsø office using computer-based Geographical Information System (GIS) software and the NIS developed the ice charting system Bifrost. This allowed the frequency of production to be increased to every weekday, with a greater focus on detailed sea ice concentrations along the ice edge and coastal zones in Eastern Greenland and in the Svalbard fjords. From 2010, the NIS has also provided a weekly austral summer ice chart for the Weddell Sea and Antarctic Peninsula. To further develop its capabilities, NIS engages in a number of national and international research projects and led the EU Horizon 2020 project, Key Environmental monitoring for Polar Latitudes and European Readiness (KEPLER). This paper summarises the overall mandate and history of the NIS, and its current activities including the current state of routine production of operational ice charts at the NIS for maritime safety in both the Arctic and Antarctic, and future development plans.
Impacts of sea ice on suspended sediment transport during heavy ice years in the Bohai Sea
Authors: Liu Siyu , Li Guangxue , Liu Shidong , Zhang Lei , Li Mengqi , Feng Qi , Xing Lvyang , Yu Di , Pan Yufeng
Published: August 2024

The Bohai Sea, known for being the lowest latitude seasonally frozen sea area in the world, experienced severe ice conditions with a 30-year recurrence period during the winter of 2009-2010. Water-sediment flux is a crucial parameter for water quality management in marine environment. Using a highly accurate three-dimensional hydrodynamic and sediment transport numerical model, the significant wave height (Hs), current velocity, suspended sediment concentration (SSC) and water-sediment flux in the Bohai Sea during ice-covered and ice-free conditions are compared. Our findings indicate that the current velocity and sediment resuspension decrease under the ice coverage, but increase at the edge of the ice. The net outflow tidal flux (TF) in winter under ice-free conditions accounts for 24.5% of the whole year. The net outflow TF increases by 32.7% during ice-covered conditions, primarily due to the pressure difference between high air pressure superimposing heavy sea ice in the northwest Bohai Sea and the low air pressure in Bohai Strait, resulting in increased ebb velocity and decreased flood velocity. The net outflow suspended sediment flux (SSF) during ice-free conditions in the winter is 2.32×109 kg, while SSF increases by 1.24 times during ice-covered conditions, leading to worse water quality in the outer sea. The decrease of TF in the southern part of the Bohai Strait and the reduction of suspended sediment concentration by nearly 15 mg/l lead to the significant decrease in SSF. This study has significance guiding value for understanding the source-sink sedimentation system and water quality research in East China Sea.
Remote sensing of suspended particulate matter concentrations in the Yellow River Estuary, China: algorithm development, long-term dynamics and driving mechanisms
Authors: Li Peng , Chen Shenliang , Ji Hongyu , Zhong Xiaojing , Qi Jiarui , Gong Xuelei , Yu Peng , Ke Yinghai
Published: August 2024

Suspended particulate matter (SPM) concentrations and associated estuarine high turbidity zones (HTZ) play crucial role in maintaining delta land building, coastal wetlands and marine ecosystems. In the background of new water-sediment delivery regime and major geomorphological transition in delta, the long-term change characteristics and driving mechanisms of SPM and HTZ in the Yellow River Estuary (YRE) are not clarified. In this study, it was found that the existing SPM models could not be adapted to the extremely turbid YRE, for which a novel SPM retrieval algorithm and HTZ extraction method were developed. Spatiotemporal dynamics of SPM and HTZ in YRE from 1984 to 2023 were investigated using 798 Landsat TM/ETM+/OLI imageries. Results indicated that our proposed SPM algorithm outperformed all the previous models of YRE (R2 > 0.95, relative percentage difference (RPD)< 22%), and high accuracies were achieved for both satellite-derived SPM (RPD = 33.01%) and HTZ (overall accuracy = 94%). Over the last four decades, both SPM concentration and HTZ distribution area in YRE had demonstrated an increasing and then decreasing trend, reaching a peak around 1996. HTZ experienced four morphological transformations involving the circular shape surrounding coast (1984–1993), the enlarged southeasterly ovoid shape (1994–2007), the circle-like shape (2007–2017) and the thin northeasterly ovoid shape (2018–2023). Riverine sediment load and strong-wind frequency controlled 67% of interannual SPM variability in the study area, and the former contributed more. Moreover, combination of weakening ocean dynamics and coastal erosion, coarsening of sediments, and coastal defense structures led to an exponential decline of SPM concentrations in northern subregion. High sediment discharge and rapid accretion of estuarine spit in 1984–1996 resulted in increased SPM concentrations in southern subregion and a quick expansion of HTZ into Laizhou Bay. With the geomorphological evolution of new and old estuaries, the high velocity zone and strong bottom shear stress zone were consequently adjusted, which explained the interannual variability of SPM and HTZ in the region. This study will contribute to understanding of the long-term evolutionary patterns of the coupled system consisting of runoff-tidal dynamics, sediment transport and geomorphological development in the estuary.
Finite element analysis and automation of a medium scale grinder applied to the manufacture of cassava starch
Authors: Oroun’Gobi Idayatou , Guo Chen Guang
Published: August 2024

This research is based on the modeling, internal structure analysis, and automation of a cassava grinding machine. For the design, the single-cylinder grinder was chosen due to its advantages, notably simplicity and versatility. The grinding method used in this type of grinder is compression. The mechanical design and simulation software first allowed for the design of the grinder and then for performing static analyses under three loads (P1 = 10000N, P2 = 15000N, and P3 = 20000N). The results of these tests led to the selection of P3 = 20000N as the straightening load, with a maximum stress value in the static analysis of 88,18 MPa and a maximum deformation of 0,000358 under the force of load P3. Additionally, the frequency analysis distinguished five resonance modes. The results for each mode concluded that no resonance would affect the machine, thus ensuring stable operation. Furthermore, fatigue, frequency, and safety factor studies showed that the machine could withstand a load of m3 = 2000 kg without damage, with a service life of 1e+09 cycles. Considering the total number of life cycles, which is 1e+09 cycles, this means that the machine will have a service life of 347000 years, with a total grinding output of 2e+12 kg of cassava and 5840000 kg per year. The factor of safety is FoS = 1,78, indicating a sufficient margin for safe operating conditions. The automation of the grinder was carried out using a GRAFCET model and a sophisticated human-machine interface (HMI), providing an additional safety point for the machine and allowing the operator to monitor the operation via a simple graphical interface. This automation enables continuous operation with minimal human intervention, thereby improving the efficiency and safety of the cassava grinding process.
The memory binding test can anticipate Alzheimer’s disease diagnosis at an early preclinical stage: a longitudinal study in the INSIGHTpreAD cohort
Authors: Raposo Pereira Filipa , George Nathalie , Dalla Barba Gianfranco , Dubois Bruno , La Corte Valentina
Published: August 2024

Introduction

Anticipating the diagnosis of Alzheimer’s disease (AD) at an early asymptomatic at-risk stage, where therapeutics can more effectively delay conscious cognitive decline, is currently among the biggest challenges in the field. Herein, we aimed to compare the capacity of the Memory Binding Test (MBT) with the official diagnostic tool, the Free and Cued Selective Reminding Test (FCSRT), to anticipate AD diagnosis at an early preclinical stage based on the associative memory component of MBT (binding), suggested as more sensitive to the emergence of subtle episodic memory (EM) deficits (AD hallmark).

Methods

We assessed the tests performance longitudinally (over 5 years) in 263 cognitively-normal elderly individuals at risk of AD (>6 months of subjective memory complaints) using linear mixed-effect models controlled for age, sex, and education. We stratified participants in 2 models: amyloid-β (Aβ)/neurodegeneration (N) model, assessing Aβ burden and neurodegeneration effect [3 groups: controls (Aβ-/N-); stable/N- (Aβ+); stable/N+ (Aβ+)]; and the stable/progressors model, assessing progression to prodromal-AD effect [2 groups: stable (Aβ+); progressors (Aβ+)], based on 15 subjects who progressed to AD during follow-up (excluded once diagnosed).

Results

Aβ burden was associated with significantly less MBT-intrusions, while Aβ burden and neurodegeneration together, with the most. Progression status had a strong negative effect on both tests performance. When compared with the FCSRT, the MBT seems to anticipate diagnosis based on a worst performance in a higher number of scores (including binding) in at least a year.

Discussion

Anticipation of diagnosis to an asymptomatic at-risk stage, while participants remain cognitively-normal according to FCSRT cut-offs and unaware of objective EM deficits, has the potential to delay the onset of AD-linked cognitive decline by applying promising therapeutics before decline becomes too advanced.
Risk factors of in-stent restenosis after carotid angioplasty and stenting: long-term follow-up study
Authors: Park Sangil , Kim Bum Joon , Choi Hye-Yeon , Chang Dae-Il , Woo Ho Geol , Heo Sung Hyuk
Published: August 2024

Background

After carotid artery angioplasty with stenting (CAS), it is unclear which risk factors are related to long-term outcomes, including in-stent restenosis (ISR). This study aimed to assess the factors associated with restenosis after CAS with a median follow-up of 35.7 months.

Materials and methods

Patients who underwent CAS from January 2013 to December 2018 were included if they had symptomatic or asymptomatic carotid artery stenosis. The carotid Doppler ultrasonography (CDU) was followed up after the procedure. We defined at least 50% restenosis using the criteria that the internal carotid artery (ICA) peak systolic velocity (PSV) was greater than 224 cm/s or the ICA to common carotid artery PSV ratio was higher than 3.4. The risk factors for ISR were also assessed.

Results

Of the 189 patients, 122 had symptomatic carotid artery stenosis, and 67 had asymptomatic carotid artery stenosis. Patients were evaluated by CDU for a median of 35.7 months (interquartile range 19.5 to 70.0). Kaplan–Meier analysis showed that the longest time to ISR was 39 months, and ISR-free was better in the asymptomatic CAS group. In all groups, ISR was independently associated with current smoker [adjusted odds ratio (aOR), 3.425; 95% confidence interval (CI), 1.086 to 10.801] and elevated ICA PSV at baseline (aOR, 1.004; 95% CI, 1.001 to 1.007).

Conclusion

Independent risk factors for ISR in the CAS group included current smoking and elevated ICA PSV at baseline. In the symptomatic CAS group, alcohol was independently associated with the ISR. ISR did not occur after 39 months from the CAS procedure in our study patients. Future studies with extended follow-up are necessary to fully understand the long-term outcomes of CAS.
Role of desmoplakin in supporting neuronal activity, neurogenic processes, and emotional-related behaviors in the dentate gyrus
Authors: Otsubo Keisuke , Sakashita Naoko , Nishimoto Yuki , Sato Yo , Tsutsui Takehisa , Kobayashi Katsunori , Suzuki Kanzo , Segi-Nishida Eri
Published: August 2024

Desmoplakin (Dsp) is a component of desmosomal cell–cell junctions that interacts with the cadherin complex and cytoskeletal intermediate filaments. In addition to its function as an adhesion component, Dsp is involved in various biological processes, such as gene expression, differentiation, and migration. Dsp is specifically expressed in the hippocampal dentate gyrus (DG) in the central nervous system. However, it is unclear how Dsp impacts hippocampal function and its related behaviors. Using an adeno-associated virus knockdown system in mice, we provide evidence that Dsp in the DG maintains hippocampal functions, including neuronal activity and adult neurogenesis, and contributes to anxiolytic-like effects. Dsp protein is mostly localized in mature granule cells in the adult DG. Dsp knockdown in the DG resulted in a lowered expression of an activity-dependent transcription factor FosB, and an increased expression of mature neuronal markers, such as calbindin. In addition, the suppression of Dsp decreases serotonin responsiveness at the DG output mossy fiber synapses and alters adult neurogenic processes in the subgranular zone of the DG. Moreover, DG-specific Dsp knockdown mice showed an increase in anxiety-like behaviors. Taken together, this research uncovers an unexplored function for Dsp in the central nervous system and suggests that Dsp in the DG may function as a regulator to maintain proper neuronal activation and adult neurogenesis, and contribute to the adaptation of emotion-related behavior.
Leveraging design of experiments to build chemometric models for the quantification of uranium (VI) and HNO3 by Raman spectroscopy
Authors: Sadergaski Luke R. , Einkauf Jeffrey D. , Delmau Laetitia H. , Burns Jonathan D.
Published: August 2024

Partial least squares regression (PLSR) and support vector regression (SVR) models were optimized for the quantification of U(VI) (10–320 g L−1) and HNO3 (0.6–6 M) by Raman spectroscopy with optimized calibration sets chosen by optimal design of experiments. The designed approach effectively minimized the number of samples in the calibration set for PLSR and SVR by selecting sample concentrations with a quadratic process model, despite complex confounding and covarying spectral features in the spectra. The top PLS2 model resulted in percent root mean square errors of prediction for U(VI), HNO3, and NO3− of 3.7%, 3.6%, and 2.9%, respectively. PLS1 models performed similarly despite modeling an analyte with a majority linear response (i.e., uranyl symmetric stretch) and another with more covarying vibrational modes (i.e., HNO3). Partial least squares (PLS) model loadings and regression coefficients were evaluated to better understand the relationship between weaker Raman bands and covarying spectral features. Support vector machine models outperformed PLS1 models, resulting in percent root mean square error of prediction values for U(VI) and HNO3 of 1.5% and 3.1%, respectively. The optimal nonlinear SVR model was trained using a similar number of samples (11) compared with the PLSR model, even though PLS is a linear modeling approach. The generic D-optimal design presented in this work provides a robust statistical framework for selecting training set samples in disparate two-factor systems. This approach reinforces Raman spectroscopy for the quantification of species relevant to the nuclear fuel cycle and provides a robust chemometric modeling approach to bolster online monitoring in challenging process environments.
Precision nutrition for type 2 diabetes in Benin: leveraging linear goal programming to optimize diets with emphasis on adequacy, affordability, accessibility, and culture
Authors: Alaofè Halimatou , Mahdavimanshadi Mahdi , Mizéhoun-Adissoda Carmelle , Okechukwu Abidemi , Hounkpatin Waliou Amoussa , Bedrick Edward John , Gninkoun Jules , Fan Neng , Ehiri John
Published: August 2024

Background

Nutrition and diet are critical to managing Type 2 diabetes (T2D). Low-income households often face challenges maintaining a healthy and balanced diet due to food insecurity, availability, and cost. To address this issue, we used a linear goal programming (LGP) model to develop nutritionally adequate, affordable, accessible, and culturally acceptable diets for persons with T2D in Benin, a French-speaking sub-Saharan country. The goal was to help persons with T2D manage their condition more effectively.

Methods

We compiled a robust list of local commonly consumed foods in Benin, and calculated their nutritional value using West African food composition tables and food costs per serving from a market survey. Using mathematical optimization techniques, we designed dietary plans that meet the daily nutrient intake recommended by the World Health Organization (WHO) to prevent chronic diseases in normal adults. While adhering to dietary constraints of T2D, we developed optimized diet plans with varying energy levels that meet all nutrient requirements while considering availability, acceptability, and budgetary constraints.

Results

Fifty-two food items and recipes were evaluated to create six low-cost daily menus. Menu 1 was the most affordable at CFA 1,127 (USD 1.88), providing 1890 kcal of energy, while Menu 6 was the most expensive at CFA 1,227 (USD 2.05), providing 1749 kcal. All the menus met the daily WHO minimum requirements for carbohydrates, fat, cholesterol, and fiber content, while other nutrients such as protein, vitamin C, and iron reached the upper limits of the acceptable value range.

Conclusion

Linear goal programming can be an effective tool in helping to obtain optimized adequate, accessible, and culturally acceptable diets at minimal cost by interpreting and translating dietary recommendations into a nutritional model, based on local market prices.
Dietary advanced glycation end-products and their associations with body weight on a Mediterranean diet and low-fat vegan diet: a randomized, cross-over trial
Authors: Kahleova Hana , Znayenko-Miller Tatiana , Motoa Giulianna , Eng Emma , Prevost Alex , Uribarri Jaime , Holubkov Richard , Barnard Neal D.
Published: August 2024

Objective

Evidence suggests that changes in dietary advanced glycation end-products (AGEs) may influence body weight, but the effects of different dietary patterns remain to be explored.

The aim of this study was to compare the effects of a Mediterranean and a low-fat vegan diet on dietary AGEs and test their association with body weight.

Materials and methods

In this randomized cross-over trial, 62 overweight adults were assigned to a Mediterranean or a low-fat vegan diet for 16-week periods in random order, separated by a 4-week washout. Body weight was the primary outcome. Three-day diet records were analyzed using the Nutrition Data System for Research software and dietary AGEs were estimated, using an established database. Statistical approaches appropriate for crossover trials were implemented.

Results

Dietary AGEs decreased by 73%, that is, by 9,413 kilounits AGE/day (95% −10,869 to −7,957); p < 0.001, compared with no change on the Mediterranean diet (treatment effect −10,303 kilounits AGE/day [95% CI −13,090 to −7,516]; p < 0.001). The participants lost 6.0 kg on average on the vegan diet, compared with no change on the Mediterranean diet (treatment effect −6.0 kg [95% CI −7.5 to −4.5]; p < 0.001). Changes in dietary AGEs correlated with changes in body weight (r = +0.47; p < 0.001) and remained significant after adjustment for total energy intake (r = +0.39; p = 0.003).

Conclusion

Dietary AGEs did not change on the Mediterranean diet but decreased on a low-fat vegan diet, and this decrease was associated with changes in body weight, independent of energy intake.

Clinical trial registration

https://clinicaltrials.gov/, identifier NCT03698955
Solitary fibrous tumor of the pineal gland: a case report and review of the literature
Authors: He Yixiao , He Pengchen , Wang Anqun , Ji Yuzhu , Xie Gang , Zou Lili
Published: August 2024

Solitary fibrous tumor (SFT) is a type of fibroblastic neoplasm that can occur in various parts of the body, with SFT of the pineal gland being exceedingly rare. We report the case of a 58-year-old male presenting with recurrent hiccups, acid reflux, and headache. Magnetic resonance imaging revealed an occupying lesion in the pineal region, suggestive of a neoplastic process. Intraoperatively, the lesion was located in the pineal region, exhibiting a grayish-red color, and was largely resected. Pathological examination confirmed the diagnosis of solitary fibrous tumor (CNS WHO Grade 1). Postoperatively, the patient was supplemented with radiotherapy, and long-term follow-up showed no signs of recurrence or metastasis.
Efficacy and safety evaluation of mixed nutrition for postoperative esophageal cancer patients in China: a meta-analysis
Authors: Liu Shui , Qiao Lin , Liu Yang , Liu Hangmei , Li Yiwen , Sun Jingbo , Chen Wei , Shang Rongguo , Zhang Lili , Liu Xiaochuan
Published: August 2024

Objective

The purpose of this study was to evaluate the clinical effect of mixed nutrition and parenteral nutrition support on postoperative patients with esophageal cancer.

Method

By searching PubMed, Web of Science, Cochrane, CNKI, Wanfang and other databases, all the literatures until March 2024 about the comparison of randomized controlled Trial (RCT) of mixed nutrition and parenteral nutrition support in postoperative patients with esophageal cancer were screened. The inclusion criteria were that the patients were from randomized controlled trials or clinical trials in China, and the patients were all diagnosed with esophageal cancer by pathological biopsy. The exclusion criteria were the literature other than the above, including repeated published literature, non-Chinese and English literature, incomplete or missing analysis data, etc. After two researchers independently screened the literature, extracted the data and evaluated the risk of bias according to the criteria, Meta-analysis was carried out with RevMan 5.4 software.

Results

A total of 11 studies were included, including 1216 patients. Meta-analysis showed that, compared with parenteral nutrition, mixed nutrition can improve the levels of transferrin, serum albumin, prealbumin and lymphocyte counts in patients with esophageal cancer after surgery, shorten the time of anal recovery of exhaust, defecation and hospital stay after surgery, and reduce the incidence of pulmonary infection, abdominal distension, incision infection and anastomotic fistula, with statistical significance between the two groups (P < 0.05). The heterogeneity of individual results in this study is relatively high, the analysis comes from clinical heterogeneity, and the publication bias is analyzed through Funnel plot. Taking the incidence of lung infection as an example, the results are evenly distributed on both sides of the Funnel plot, and the publication bias has little impact on the results of the study.

Conclusion

Compared with parenteral nutrition, mixed nutrition can improve the prognosis of postoperative patients with esophageal cancer and reduce the incidence of related adverse events.
Tissue, cellular, and molecular level determinants for eye lens stiffness and elasticity
Authors: Cheng Catherine
Published: August 2024

The eye lens is a transparent, ellipsoid tissue in the anterior chamber that is required for the fine focusing of light onto the retina to transmit a clear image. The focusing function of the lens is tied to tissue transparency, refractive index, and biomechanical properties. The stiffness and elasticity or resilience of the human lens allows for shape changes during accommodation to focus light from objects near and far. It has long been hypothesized that changes in lens biomechanical properties with age lead to the loss of accommodative ability and the need for reading glasses with age. However, the cellular and molecular mechanisms that influence lens biomechanical properties and/or change with age remain unclear. Studies of lens stiffness and resilience in mouse models with genetic defects or at advanced age inform us of the cytoskeletal, structural, and morphometric parameters that are important for biomechanical stability. In this review, we will explore whether: 1) tissue level changes, including the capsule, lens volume, and nucleus volume, 2) cellular level alterations, including cell packing, suture organization, and complex membrane interdigitations, and 3) molecular scale modifications, including the F-actin and intermediate filament networks, protein modifications, lipids in the cell membrane, and hydrostatic pressure, influence overall lens biomechanical properties.
Efficacy and safety of fospropofol disodium sedation for same-day bidirectional endoscopy in elderly patients: protocol for a prospective, single-center, randomized, double-blind, non-inferiority trial
Authors: Zhao Zhe , Jin Xiaogao , Li Yong , Wang Xiaofeng , Cui Yongchen , Zhang Bing , Kang Yu , Zhang Guangming , Chu Qinjun , Zhang Junfeng
Published: August 2024

Introduction

Fospropofol disodium is a novel prodrug that has improved pharmacokinetic and pharmacodynamic properties when compared with propofol. This trial aims to compare the efficacy and safety of fospropofol versus propofol sedation for same-day bidirectional endoscopy in elderly patients.

Methods and analysis

This is a prospective, single-center, double-blind, randomized, propofol-controlled, non-inferiority trial. A total of 256 patients aged 65 years or older, who are scheduled for same-day bidirectional endoscopy under sedation, will be randomly allocated, in a 1:1 ratio, to either fospropofol group or propofol group (n = 128 in each group). All patients will receive analgesic pre-treatment with sufentanil 5 μg. Two minutes later, an initial bolus dose of fospropofol 6.5 mg/kg or 1.5 mg/kg propofol and supplemental doses of fospropofol 1.6 mg/kg or 0.5 mg/kg propofol will be titrated as needed to achieve target sedation levels during the procedures. The primary outcome is the success rate of same-day bidirectional endoscopy. Secondary outcomes include the time to successful induction of sedation, duration, time to being fully alert, time to patient discharge, endoscopist satisfaction, patient satisfaction, and the top-up frequency and dosage of sedative medications. The safety endpoints consist of adverse events concerning cough reflex, gag reflexes, body movement, muscular tremor, and pain on injection. Sedation-related AEs, including episodes of desaturation, severe desaturation (SpO2 < 90%), hypotension, severe hypotension (decrease in MBP ≥30% of baseline), and bradycardia, will also be recorded. Data will be analyzed on an intention-to-treat basis.

Discussion

We hypothesize that the efficacy and safety of fospropofol sedation for elderly patients undergoing same-visit bidirectional endoscopy will not be inferior to that of propofol. Our findings will potentially provide a new sedation regimen for same-visit bidirectional endoscopy in elderly patients.

Clinical Trial Registration

clinicaltrials.gov, identifier NCT02875639
Qianggu concentrate: unlocking bone protection power via antioxidative SIRT1/NRF2/HO-1 pathways in type 2 diabetic osteoporosis
Authors: Wang Kaili , Dang Xiang , Wang Yanyan , Yang Qing , Zhang Tingting , Yang Peng , Yuan Ling , Xu Rongming , Dang Yuqi , Nan Yi
Published: August 2024

Background

Qianggu Concentrate (QGHJ), a traditional Chinese medicine, is extensively used to treat Type 2 Diabetic Osteoporosis (T2DOP). Despite its widespread use, research on its therapeutic mechanisms within T2DOP is notably scarce.

Objective

To explore QGHJ’s osteoprotection in T2DOP rats and BMSCs, focusing on the antioxidant activation of SIRT1/NRF2/HO-1 and NRF2 nuclear migration.

Methods

QGHJ constituent analysis was performed using UPLC-HRMS. Safety, bone-health efficacy, and glucose metabolic effects in T2DOP rats were evaluated via general condition assessments, biomarker profiling, micro-CT, biomechanics, staining methods, and ELISA, supplemented by RT-qPCR and Western blot. BMSCs’ responses to QGHJ under oxidative stress, including viability, apoptosis, and osteogenic differentiation, were determined using CCK-8, flow cytometry, ALP/ARS staining, and molecular techniques. The modulation of the SIRT1/NRF2/HO-1 pathway by QGHJ was explored through oxidative stress biomarkers, immunofluorescence, and Western blot assays.

Results

UPLC-HRMS identified flavonoids, monoterpenes, and isoflavones as QGHJ’s key compounds. In vivo, QGHJ proved safe and effective for T2DOP rats, enhancing bone mineral density, microenvironment, and biomechanical properties without impairing vital organs. It modulated bone markers PINP, TRACP 5b, RUNX2 and PPARγ, favoring bone anabolism and reduced catabolism, thus optimizing bone integrity. QGHJ also regulated glycemia and mitigated insulin resistance. In vitro, it preserved BMSCs’ viability amidst oxidative stress, curbed apoptosis, and fostered osteogenesis with regulated RUNX2/PPARγ expression. Mechanistic insights revealed QGHJ activated the SIRT1/NRF2/HO-1 pathway, augmented NRF2 nuclear translocation, and enhanced the antioxidative response, promoting bone health under stress.

Conclusion

In T2DOP rat and BMSCs oxidative stress models, QGHJ’s bone protection is anchored in its antioxidative mechanisms via the SIRT1/NRF2/HO-1 pathway activation and NRF2 nuclear translocation.
Time-restricted eating, the clock ticking behind the scenes
Authors: Ribas-Latre Aleix , Fernández-Veledo Sonia , Vendrell Joan
Published: August 2024

Introduction

Maintaining metabolic balance relies on accumulating nutrients during feeding periods and their subsequent release during fasting. In obesity and metabolic disorders, strategies aimed at reducing food intake while simulating fasting have garnered significant attention for weight loss. Caloric restriction (CR) diets and intermittent fasting (IF) interventions have emerged as effective approaches to improving cardiometabolic health. Although the comparative metabolic benefits of CR versus IF remain inconclusive, this review focuses on various forms of IF, particularly time-restricted eating (TRE).

Methods

This study employs a narrative review methodology, systematically collecting, synthesizing, and interpreting the existing literature on TRE and its metabolic effects. A comprehensive and unbiased search of relevant databases was conducted to identify pertinent studies, including pre-clinical animal studies and clinical trials in humans. Keywords such as “Obesity,” “Intermittent Fasting,” “Time-restricted eating,” “Chronotype,” and “Circadian rhythms” guided the search. The selected studies were critically appraised based on predefined inclusion and exclusion criteria, allowing for a thorough exploration and synthesis of current knowledge.

Results

This article synthesizes pre-clinical and clinical studies on TRE and its metabolic effects, providing a comprehensive overview of the current knowledge and identifying gaps for future research. It explores the metabolic outcomes of recent clinical trials employing different TRE protocols in individuals with overweight, obesity, or type II diabetes, emphasizing the significance of individual chronotype, which is often overlooked in practice. In contrast to human studies, animal models underscore the role of the circadian clock in mitigating metabolic disturbances induced by obesity through time-restricted feeding (TRF) interventions. Consequently, we examine pre-clinical evidence supporting the interplay between the circadian clock and TRF interventions. Additionally, we provide insights into the role of the microbiota, which TRE can modulate and its influence on circadian rhythms.