bluuebunny/crossref_metadata_2025 huggingface dataset:
Determining the mechanism of pulsatilla decoction for treating gastric cancer: a network pharmacology-based study
Authors: Huang Siqi , Qu Manying , Chen Xiaowu , Yu Shaochen , Kong Fanhua
Published: June 2023

Background and aim

Gastric cancer (GC) is a prevalent malignancy worldwide. Pulsatilla decoction (PD), a traditional Chinese medicine formula, can treat inflammatory bowel disease and cancers. In this study, we explored the bioactive components, potential targets, and molecular mechanisms of PD in the treatment of GC.

Methods

We conducted a thorough search of online databases to gather gene data, active components, and potential target genes associated with the development of GC. Subsequently, we conducted bioinformatics analysis utilizing protein–protein interaction (PPI), network construction, and Kyoto Encyclopedia of Genes and Genomes (KEGG) to identify potential anticancer components and therapeutic targets of PD. Finally, the efficacy of PD in treating GC was further validated through in vitro experiments.

Results

Network pharmacological analysis identified 346 compounds and 180 potential target genes associated with the impact of PD on GC. The inhibitory effect of PD on GC may be mediated through modulation of key targets such as PI3K, AKT, NF-κB, FOS, NFKBIA, and others. KEGG analysis showed that PD mainly exerted its effect on GC through the PI3K–AKT, IL-17, and TNF signaling pathways. Cell viability and cell cycle experiments showed that PD could significantly inhibit proliferation and kill GC cells. Moreover, PD primarily induces apoptosis in GC cells. Western blotting analysis confirmed that the PI3K–AKT, IL-17, and TNF signaling pathways are the main mechanisms by which PD exerts its cytotoxic effects on GC cells.

Conclusion

We have validated the molecular mechanism and potential therapeutic targets of PD in treating GC through network pharmacological analysis, thereby demonstrating its anticancer efficacy against GC.
Primary intracranial sarcomas: a clinicopathological investigation
Authors: Cai Yu-Xiang , Liu Jin-Sheng , Xu Jian , He Yong-Ze , Zhang Huai-Nian , Tian Su-Fang , Li Zhi-Qiang
Published: June 2023

Background

The purpose of this study is to present a series of primary intracranial sarcomas (PIS), a rare type of tumor of the central nervous system, in order to improve our understanding of the disease. These tumors are heterogeneous and prone to recurrence after resection, exhibiting a high mortality rate. As PIS has yet to be understood and studied on a large scale, it is vital for further evaluation and research.

Methods

Our study included 14 cases of PIS. The patients’ clinical, pathological, and imaging features were retrospectively analyzed. Additionally, targeted DNA next-generation sequencing (NGS) was applied for the 481-gene panel to detect gene mutations.

Results

The average age for PIS patients was 31.4 years. Headache (7, 50.0%) was the most common symptom leading to the hospital visit. Twelve cases had PIS located in the supratentorial area and two in the cerebellopontine angle region. The maximum tumor diameter ranged from 19.0 mm to 130.0 mm, with an average diameter of 50.3 mm. Pathological types of tumors were heterogeneous, with chondrosarcoma being the most common, followed by fibrosarcoma. Eight of the 10 PIS cases that underwent MRI scanning showed gadolinium enhancement; 7 of these cases were heterogeneous, and 1 of them was garland-like. Targeted sequencing was performed in two cases and identified mutations in genes such as NRAS, PIK3CA, BAP1, KDR, BLM, PBRM1, TOP2A, DUSP2, and CNV deletions of SMARCB1. Additionally, the SH3BP5::RAF1 fusion gene was also detected. Of the 14 patients, 9 underwent a gross total resection (GTR), and 5 chose subtotal resection. Patients who underwent GTR displayed a trend toward superior survival. Among the 11 patients with available follow-up information, one had developed lung metastases, three had died, and eight were alive.

Conclusion

PIS is extremely rare compared to extracranial soft sarcomas. The most common histological type of intracranial sarcoma (IS) is chondrosarcoma. Patients who underwent GTR of these lesions showed improved survival rates. Recent advancements in NGS aided in the identification of diagnostic and therapeutic PIS-relevant targets.
Bronchoscopic instillation of amphotericin B is a safe and effective measure to treat pulmonary mycosis
Authors: Yang Lei , Yang Changqing , Wan Nansheng , Xie Wei , Tian Yu , Xiao Yangbao , Luo Li , Chen Enguo , Zhang Jisong , Wang Xiaoping , Xu Li , Wang Xingguang , Zhou Yunzhi , Guo Lu , Zou Jun , Liu Xingren , Wei Xuguang , Wang Yubao , Feng Jing
Published: June 2023

Background and objectives: In recent years, there has been a significant increase in the prevalence of pulmonary mycosis disease, and its mortality has increased. There are very few studies on treating pulmonary mycosiss with bronchoscopic instillation of amphotericin B. This study investigated the clinical efficacy and safety of bronchoscopic instillation of amphotericin B for treating pulmonary mycosiss.

Methods: This was a multi-centre, retrospective clinical study of 80 patients with pulmonary mycosiss who were treated with bronchoscopic instillation of amphotericin B. The efficacy and safety of this treatment were evaluated.

Results: Eighty patients were included {51 males; mean [standard deviation (SD)] age, 46 (15.9) years}. The most common underlying cause was haematological malignancy (73.75%). The mean number of bronchoscopic instillations of amphotericin B was 2.4 (SD 1.5). In terms of treatment success, 58 (72.5%) patients achieved complete or partial changes on imaging after treatment. A total of 62 (77.5%) patients achieved complete or partial changes on imaging and/or local limitation of the mycosis infection. Seventy-six (95%) patients achieved complete or partial changes on imaging and/or local limitation of mycosis infection and/or an immunotherapy time window. The efficacy rates for treatment of Aspergillus and Mucor infections in terms of the three treatment success criteria described above were 73.81% vs. 63.64%, 80.95% vs. 72.73%, and 92.86% vs. 90.91%, respectively.

Conclusion: Bronchoscopic instillation of amphotericin B is safe and effective for treatment of pulmonary mycosiss.
Value evaluation of knee joint sports injury detection model-aided diagnosis based on machine learning
Authors: Liu Hao
Published: June 2023

Athletes often suffer from knee joint injuries because they often use the knee joint to exert force during training. This paper aims to analyze and discuss the auxiliary diagnosis of the knee joint sports injury detection model based on machine learning. This paper expounds the treatment method of knee joint injury, and proposes a machine learning algorithm. On the basis of this research, the auxiliary diagnosis experiment of the knee joint sports injury detection model is analyzed. The experimental results show that after 3 months of machine learning-based rehabilitation training, there is a significant difference in the duration of the balance pad before and after the table tennis players practice. The duration of the athletes on the balance mat has increased, and the increase is relatively large. Among them, the average duration of female athletes on the balance mat increased from 75.5 seconds before training to 141.9 seconds after training, while the average duration of male athletes on the balance mat increased from 66.7 seconds before training to 136.8 seconds after training. Studies have shown that machine learning-based rehabilitation physical training can significantly improve athletes' endurance on balance mats and can improve knee function scores. In summary, machine learning-based rehabilitation physical training can effectively improve knee joint injuries.
Rapid automatic detection of water Ca, Mg elements using laser-induced breakdown spectroscopy
Authors: Zhu Yijun , Ma Shixiang , Yang Guiyan , Tian Hongwu , Dong Daming
Published: June 2023

Ca, Mg, as the main components in water, are not only essential nutrients for life but are also an important index of water hardness. Rapid detection of the element content is of great significance for water quality evaluation and nutrient balance in planting and breeding water. Traditional laboratory analysis methods are time consuming and involve complex procedures. Ion cross sensitivity and response drift also limit the application of online sensors. These disadvantages are not in line with the precise control and rapid response required for practical applications. Here, we propose a rapid automatic Ca, Mg ion concentration detection method using Laser-induced breakdown spectroscopy. Based on a cation exchange membrane, an automatic control system integrating water sampling, hybrid reacting, attachment drying and spectra measuring was constructed. The testing time for one sample could be compressed to 5 min. Consequently, it met the requirements of accuracy and timeliness of water element regulation. The proposed system can provide new technical means for large-scale aquaculture, soilless cultivation of facilities and other scenarios aiming at accurate measurement and control of elements in water body.
Sensitivity of Clarireedia spp. to benzimidazoles and dimethyl inhibitors fungicides and efficacy of biofungicides on dollar spot of warm season turfgrass
Authors: Ghimire Bikash , Aktaruzzaman Md. , Chowdhury Shukti R. , Spratling Willis T. , Vermeer C. Brian , Buck James W. , Martinez-Espinoza Alfredo D. , Bahri Bochra A.
Published: June 2023

Dollar spot caused by Clarireedia spp. (formerly Sclerotinia homoeocarpa) is an economically destructive fungal disease of turfgrass that can significantly compromise turf quality, playability, and aesthetic value. Fungicides are frequently used to manage the disease but are costly and potentially unfavorable to the environment. Repeated use of some active ingredients has resulted in reduced efficacy on C. jacksonii causing dollar spot in cool-season turfgrasses in the US. Experiments were conducted to study fungicide sensitivity of Clarireedia spp. as well as to develop alternatives to fungicides against dollar spot on warm-season turfgrass in Georgia. First, 79 isolates of Clarireedia spp. collected across the state were tested on fungicide-amended agar plates for their sensitivity to thiophanate-methyl (benzimidazole) and propiconazole (dimethyl inhibitor). Seventy-seven isolates (97.5%) were sensitive (0.001 to 0.654 μg/mL) and two isolates (2.5%) were found resistant (>1000 μg/mL) to thiophanate-methyl. However, in the case of propiconazole, 27 isolates (34.2%) were sensitive (0.005 to 0.098 μg/mL) while 52 isolates (65.8%) were resistant (0.101 to 3.820 μg/mL). Next, the efficacy of three bio- and six synthetic fungicides and ten different combinations were tested in vitro against C. monteithiana. Seven bio- and synthetic fungicide spray programs comprising Bacillus subtilis QST713 and propiconazole were further tested, either alone or in a tank mix in a reduced rate, on dollar spot infected bermudagrass ‘TifTuf’ in growth chamber and field environments. These fungicides were selected as they were found to significantly reduce pathogen growth up to 100% on in vitro assays. The most effective spray program in growth chamber assays was 100% B. subtilis QST713 in rotation with 75% B. subtilis QST713 + 25% propiconazole tank mix applied every 14 days. However, the stand-alone application of the biofungicide B. subtilis QST713 every seven days was an effective alternative and equally efficacious as propiconazole, suppressing dollar spot severity and AUDPC up to 75%, while resulting in acceptable turf quality (>7.0) in field experiments. Our study suggests that increased resistance of Clarireedia spp. to benzimidazoles and dimethyl inhibitors warrants continuous surveillance and that biofungicides hold promise to complement synthetic fungicides in an efficacious and environmentally friendly disease management program.
Transcriptome profiles of rice roots under simulated microgravity conditions and following gravistimulation
Authors: Kuya Noriyuki , Nishijima Ryo , Kitomi Yuka , Kawakatsu Taiji , Uga Yusaku
Published: June 2023

Root system architecture affects the efficient uptake of water and nutrients in plants. The root growth angle, which is a critical component in determining root system architecture, is affected by root gravitropism; however, the mechanism of root gravitropism in rice remains largely unknown. In this study, we conducted a time-course transcriptome analysis of rice roots under conditions of simulated microgravity using a three-dimensional clinostat and following gravistimulation to detect candidate genes associated with the gravitropic response. We found that HEAT SHOCK PROTEIN (HSP) genes, which are involved in the regulation of auxin transport, were preferentially up-regulated during simulated microgravity conditions and rapidly down-regulated by gravistimulation. We also found that the transcription factor HEAT STRESS TRANSCRIPTION FACTOR A2s (HSFA2s) and HSFB2s, showed the similar expression patterns with the HSPs. A co-expression network analysis and an in silico motif search within the upstream regions of the co-expressed genes revealed possible transcriptional control of HSPs by HSFs. Because HSFA2s are transcriptional activators, whereas HSFB2s are transcriptional repressors, the results suggest that the gene regulatory networks governed by HSFs modulate the gravitropic response through transcriptional control of HSPs in rice roots.
Mother phubbing and adolescents’ problematic SNS use: the mediating role of perceived burdensomeness and the moderating role of need to belong
Authors: Wang Pengcheng , Ouyang Mingkun , Yin Yulong , Li Biao
Published: June 2023

There is a rapidly growing academic interest on parental phubbing, however, little research has explored the association between mother phubbing and adolescent problematic social networking sites use (PSNSU), the underlying mediating and moderating effects in this relationship are also in need to be uncovered. The present study examined whether mother phubbing would be positively related to adolescent PSNSU, whether perceived burdensomeness would mediate this relationship, and whether need to belong would moderate the associations between mother phubbing and adolescent PSNSU. The hypothesized research model was examined among 3,915 Chinese adolescents (47% of them were boys, mean age = 16.42 ± 0.77 years). The results showed that mother phubbing was positively associated with adolescent PSNSU and perceived burdensomeness mediated this association. Furthermore, need to belong moderated the relationship between perceived burdensomeness and PSNSU, the relationship between mother phubbing and perceived burdensomeness, and the relationship between mother phubbing and PSNSU.
Post-traumatic-stress in the context of childhood maltreatment: pathways from attachment through mentalizing during the transition to parenthood
Authors: Ensink Karin , Bégin Michaël , Martin-Gagnon Gabriel , Biberdzic Marko , Berthelot Nicolas , Normandin Lina , Fonagy Peter , Bernazzani Odette , Borelli Jessica L.
Published: June 2023

Introduction

This study aimed to clarify the role of mentalizing in pathways from attachment to Post Traumatic Stress Symptoms (PTSS) in survivors of childhood maltreatment (CM). We focused on the transition to parenting, a critical period for reworking parenting representations to reduce intergenerational maltreatment cycles.

Method

Study participants included 100 pregnant CM survivors. We assessed PTSS with the SCID and attachment and mentalizing with the Adult Attachment Interview (AAI), which was rated for Attachment and Reflective Functioning (RF).

Results

Regarding Re-experiencing trauma symptoms, the results of the path analysis were consistent with mediation. CM survivors' mentalizing about their early relationships with their parents (RF-Other) directly impacted Re-experiencing trauma symptoms, and attachment had an effect on Re-experiencing trauma symptoms through mentalizing (RF-Other). Regarding Arousal/Reactivity symptoms, the results of the pathways analysis were consistent with partial mediation by mentalizing about early relationships with parents (RF-Other). In addition to the pathway from attachment via mentalizing (RF-Other) to Arousal/Reactivity, the pathway between attachment and Arousal/Reactivity also remained significant.

Discussion

This study provides new evidence of a mentalizing and attachment model of PTSS in CM survivors. The findings indicate that increased mentalizing about early relationships with parents is an important process associated with lower PTSS. Finally, we discuss the implications of developing interventions for CM survivors to reduce PTSS. Scaffolding the development of mentalizing regarding attachment relationships in which CM occurred may help CM survivors reduce the intrusion of traumatic memories and decrease trauma-related arousal and reactivity symptoms. Interventions to help CM survivors mentalize regarding parents and attachment relationships in which trauma occurred may be particularly important during the transition to parenting when activation of representations of parenting can trigger PTSS.
Detecting microstructural alterations of cerebral white matter associated with breast cancer and chemotherapy revealed by generalized q-sampling MRI
Authors: Chen Vincent Chin-Hung , Chuang Wei , Chen Chien-Wei , Tsai Yuan-Hsiung , McIntyre Roger S. , Weng Jun-Cheng
Published: June 2023

Objective

Previous studies have discussed the impact of chemotherapy on the brain microstructure. There is no evidence of the impact regarding cancer-related psychiatric comorbidity on cancer survivors. We aimed to evaluate the impact of both chemotherapy and mental health problem on brain microstructural alterations and consequent cognitive dysfunction in breast cancer survivors.

Methods

In this cross-sectional study conducted in a tertiary center, data from 125 female breast cancer survivors who had not received chemotherapy (BB = 65; 49.86 ± 8.23 years) and had received chemotherapy (BA = 60; 49.82 ± 7.89 years) as well as from 71 age-matched healthy controls (47.18 ± 8.08 years) was collected. Chemotherapeutic agents used were docetaxel and epirubicin. We used neuropsychological testing and questionnaire to evaluate psychiatric comorbidity, cognitive dysfunction as well as generalized sampling imaging (GQI) and graph theoretical analysis (GTA) to detect microstructural alterations in the brain.

Findings

Cross-comparison between groups revealed that neurotoxicity caused by chemotherapy and cancer-related psychiatric comorbidity may affect the corpus callosum and middle frontal gyrus. In addition, GQI indices were correlated with the testing scores of cognitive function, quality of life, anxiety, and depression. Furthermore, weaker connections between brain regions and lower segregated ability were found in the post-treatment group.

Conclusion

This study suggests that chemotherapy and cancer-related mental health problem both play an important role in the development of white matter alterations and cognitive dysfunction.
Children's independent mobility during dark hours: a scoping review
Authors: Litsmark Anna , Rahm Johan , Mattsson Pimkamol , Johansson Maria
Published: June 2023

Introduction

Independent mobility is every child's right and has implications for their health, wellbeing, and development. This scoping review addresses children's needs and experiences of light conditions in their everyday outdoor life. The review examines peer-reviewed scientific literature that analyses associations between different light conditions and children's independent mobility (CIM) during dark hours.

Methods

By formulating a Boolean search string, including terms related to children independent mobility, light and outdoor environment, five scientific databases were searched. The search resulted in 67 eligible papers that were analyzed through an inductive, thematic analysis.

Results

Four overarching themes representing the researched topics of the effects of light conditions with importance for CIM during dark hours were identified: (1) physical activity (PA) and active travel, (2) outdoor activities and place use, (3) safety perception, and (4) outdoor risks. The findings highlight that darkness constitutes a major obstacle for CIM, and that fear of darkness is common among children. It restricts the degree of CIM and influences children's safety perception as well as how they navigate through public places outdoors. The findings show that the type and design of outdoor settings during dark hours and children's familiarity with places during daytime could play a role in the degree of CIM after dark. The presence of outdoor lighting is related to children's increased PA and active travel, and outdoor lighting seems to also influence children's place use and interaction with the environment. The presence and extent of outdoor lighting and lighting quality may play a role in children's safety perception, which in turn can influence CIM.

Discussion

The findings suggest that promoting CIM during dark hours might not only contribute to the accumulation of children's PA, confidence, and skills, but also support mental health. The understanding of children's perspectives on the quality of outdoor lighting needs to be deepened to support CIM. Highlighting the child perspective would aid the development of current recommendations for outdoor lighting and the implementation of the Agenda 2030 of ensuring healthy lives and promoting wellbeing for all at all ages, and making cities inclusive, safe, resilient and sustainable throughout the day and seasons.
Nearly one out of every five adult TB patients suffered from food insecurity in Grawa District, Eastern Ethiopia: a multicenter facility-based cross-sectional study
Authors: Abdulla Jabir H. , Gebremichael Berhe , Maruta Melat B. , Yuye Ibsa , Mohammed Abdusalam , Debella Adera , Mussa Ibsa
Published: June 2023

Background

Despite a dramatic decline in tuberculosis mortality over the past 10 years, tuberculosis is still the leading cause of death globally. In the last 2 years, tuberculosis has affected an estimated 10 million individuals, and 1.4 million people have died worldwide. In Ethiopia, the weight of the problem is less known in the study area. As a result, the purpose of this study was to assess food insecurity and associated factors among adult patients with tuberculosis attending public health facilities in Grawa district, Eastern Ethiopia.

Methods

A multicenter facility-based cross-sectional study was conducted from 01 March to 31 March 2022, among 488 randomly selected adult tuberculosis patients on treatment follow-up at public health facilities in Grawa district, Eastern Ethiopia. Data were collected using a pretested structured questionnaire through a face-to-face interview and document review, entered into EpiData version 3.1, and analyzed using SPSS version 25. The prevalence was reported using a 95% confidence interval (CI) and summary measures. Predictors were assessed using a multivariable logistic regression analysis model and reported using an adjusted odds ratio (AOR) with 95% CI. Statistical significance was declared at a p-value of <0.05.

Results

Overall, the prevalence of food insecurity among the study participants was 19.5%, with a 95% CI (15.8%, 23.2%). Factors such as being male [AOR = 0.58, 95% CI: (0.34, 0.97)], being married [AOR = 2.93, 95% CI: (1.33, 6.47)], being merchant [AOR = 0.22, 95% CI: (0.04, 0.67)], having low wealth quintiles [AOR = 2.10,95%CI:(1.04–4.23)], receiving anti-TB treatment for two or fewer months [AOR = 0.48, 95% CI: (0.26–0.91)], using khat [AOR = 2.18, 95% CI: (1.29, 3.70)], and owning livestock (AOR = 0.56, 95% CI: 0.29–0.94) were significantly associated with food insecurity.

Conclusions

According to this study, nearly one out of every five adults TB patients is food insecure. Factors such as being male, being married, being merchant, having low wealth quintiles, receiving anti-TB treatment for two or less months, those who chew mKhat and having a livestock were significantly associated with food insecurity. As a result, all stakeholders and concerned entities should prioritize improving the livelihood of TB patients through social security system programs, which are critical to the success of TB control and prevention efforts.
Effectiveness of SARS-CoV-2 vaccines against Omicron infection and severe events: a systematic review and meta-analysis of test-negative design studies
Authors: Song Shangchen , Madewell Zachary J. , Liu Mingjin , Longini Ira M. , Yang Yang
Published: June 2023

Background

A rapidly growing body was observed of literature evaluating the vaccine effectiveness (VE) against Omicron in test-negative design studies.

Methods

We systematically searched papers that evaluated VE of SARS-CoV-2 vaccines on PubMed, Web of Science, Cochrane Library, Google Scholar, Embase, Scopus, bioRxiv, and medRxiv published from November 26th, 2021, to June 27th, 2022 (full doses and the first booster), and to January 8th, 2023 (the second booster). The pooled VE against Omicron-associated infection and severe events were estimated.

Results

From 2,552 citations identified, 42 articles were included. The first booster provided stronger protection against Omicron than full doses alone, shown by VE estimates of 53.1% (95% CI: 48.0–57.8) vs. 28.6% (95% CI: 18.5–37.4) against infection and 82.5% (95% CI: 77.8–86.2) vs. 57.3% (95% CI: 48.5–64.7) against severe events. The second booster offered strong protection among adults within 60 days of vaccination against infection (VE=53.1%, 95% CI: 48.0–57.8) and severe events (VE=87.3% (95% CI: 75.5–93.4), comparable to the first booster with corresponding VE estimates of 59.9% against infection and 84.8% against severe events. The VE estimates of booster doses against severe events among adults sustained beyond 60 days, 77.6% (95% CI: 69.4–83.6) for first and 85.9% (95% CI: 80.3–89.9) for the second booster. The VE estimates against infection were less sustainable regardless of dose type. Pure mRNA vaccines provided comparable protection to partial mRNA vaccines, but both provided higher protection than non-mRNA vaccines.

Conclusions

One or two SARS-CoV-2 booster doses provide considerable protection against Omicron infection and substantial and sustainable protection against Omicron-induced severe clinical outcomes.
Factors associated with heartworm preventative use in the golden retriever lifetime study
Authors: Wisnieski Lauren , Faulkner Vina , Faulkner Charles
Published: June 2023

Introduction

Heartworm disease is preventable with use of heartworm preventatives, but the reported prevalence of heartworm preventative use in the United States is low, some estimates falling around 50% of dogs. However, there are very few estimates of prevalence and its associated factors.

Methods

We aimed to estimate prevalence and evaluate factors, including vaccination status, demographics, lifestyle, physical conditions, medications and supplements, and environment and living conditions, for their association with heartworm preventative use in a large dataset from the Golden Retriever Lifetime Study (N = 2,998). Due to the large number of predictors evaluated, we built a bootstrapped elastic net logistic regression model, which is robust to overfitting and multicollinearity. Variables were evaluated by calculating covariate stability (>80%) and statistical significance (p<0.02).

Results

In our sample, the prevalence of heartworm use was 39.5%. In our elastic net model, receiving vaccinations (rabies, Bordetella, or any other vaccine), being located in the Southern U.S., being altered, having an infectious disease or ear/ nose/throat system disease diagnosis, being on heartworm preventatives in the past, currently being on tick preventative, having sun exposure in an area with concrete flooring, living in a house with more rooms with carpeted floors, and spending time on hardwood flooring inside were associated with greater odds of heartworm preventative use. Supplementation use and being in the top quartile of height were associated with lower odds of heartworm preventative use.

Discussion

The explanatory factors we identified can be used to improve client communication. In addition, target populations for educational interventions and outreach can be identified. Future studies can validate the findings in a more diverse population of dogs.
Electron Beam Irradiation to Control Rhizoctonia solani in Potato
Authors: Chulikova Natalya , Malyuga Anna , Borshchegovskaya Polina , Zubritskaya Yana , Ipatova Victoria , Chernyaev Alexander , Yurov Dmitry , Zolotov Sergei , Nikitchenko Alexander , Bliznyuk Ulyana , Rodin Igor
Published: June 2023

This study focuses on the influence of pre-planting irradiation on the development, health, and yield of seed potatoes infected with Rhizoctonia solani. The research was prompted by the need to ensure crop security and sustainability in the modern-day environment, which calls into question the future sufficiency of crop yields. Considering that the focus has shifted to non-chemical methods of crop treatment at all plant development stages in response to more stringent regulations governing potato production, it is particularly important to refine physics-based methods to suppress fungal diseases caused by Rhizoctonia solani. Irradiation of tubers with 20–150 Gy inhibited the potato development phases and the doses exceeding 150 Gy completely suppressed the potato sprouting. Doses ranging from 20 Gy to 100 Gy decreased the quantity of large tubers by 10–20% on average while the number of medium and small tubers increased by 5–15% and 3–10%, respectively. Irradiation of seed potatoes also decreased the sclerotia and non-sclerotia forms of diseases caused by Rhizoctonia solani in the harvested tubers. It was found that 1 MeV electron irradiation with doses ranging from 20 Gy to 30 Gy is the most efficient for the pre-planting treatment of seed potatoes since the penetration of low-energy accelerated electrons into the upper layers of potato tubers ensures the suppression of diseases caused by Rhizoctonia solani by at least 10% from the value of non-irradiated samples and prevents the reduction of total yield allowing for a maximum of 25% loss.
Dodder Parasitism Leads to the Enrichment of Pathogen Alternaria and Flavonoid Metabolites in Soybean Root
Authors: Luo Wen , Li Yuanli , Luo Ruiping , Wei Gehong , Liu Yongxin , Chen Weimin
Published: June 2023

Dodders (Cuscuta chinensis) are rootless and holoparasitic herbs that can infect a variety of host plants, including the vitally important economic and bioenergy crop soybean (Glycine max). Although dodder parasitism severely affects the physiology of host plants, little is known about its effects on fungal communities and root secondary metabolites in hosts. In this study, variations in root-associated fungal communities and root metabolites of soybean under different parasitism conditions were investigated using ITS rRNA gene sequencing and UPLC–MS/MS metabolome detection technologies. The results showed that dodder parasitism significantly altered the composition and diversity of the fungal communities in the rhizosphere and endosphere of soybean. The relative abundance of the potential pathogenic fungus Alternaria significantly increased in the root endosphere of dodder-parasitized soybean. Furthermore, correlation analysis indicated that the fungal community in the root endosphere was susceptible to soil factors under dodder parasitism. Meanwhile, the content of soil total nitrogen was significantly and positively correlated with the relative abundance of Alternaria in the rhizosphere and endosphere of soybean. Metabolomic analysis indicated that dodder parasitism altered the accumulation of flavonoids in soybean roots, with significant upregulation of the contents of kaempferol and its downstream derivatives under different parasitism conditions. Taken together, this study highlighted the important role of dodder parasitism in shaping the fungal communities and secondary metabolites associated with soybean roots, providing new insights into the mechanisms of multiple interactions among dodder, soybean, microbial communities and the soil environment.
Modeling of Typically Shaped Corn Seeds and Calibration of the Coefficient of Rolling Friction
Authors: Shi Linrong , Zhao Wuyun , Rao Gang , Guo Junhai , Wang Zun
Published: June 2023

The shape of corn seeds not being spherical affects their mobility. This study focuses on modeling the typically shaped corn seeds and calibrating the coefficient of rolling friction for different shape types to improve simulation reliability. By analyzing the corn seed shape characteristics and size statistics, this study establishes a classification system that enables the determination of the average value and quantity of different types of corn seed using the spherical granule cluster method. The discrete element method is used to model simplified corn models, and contact parameters are validated through two types of repose angle and a seed metering experiment. In the collapse repose experiment, the relative error between the simulation and the experiment was only 0.72%, while the relative error in another repose experiment was 0.2%. The verification experiment for the metering of seeds showed that the relative error between the simulation and the experiment was below 15% at both low and high speeds, and the multi-grain rate error was less than 10%. This shows that the method proposed in this paper is somewhat accurate.
Effect of Oral Administration with Lactobacillus plantarum CAM6 on the Hematological Profile, Relative Weight of Digestive Organs, and Cecal Traits in Growing Pigs
Authors: Betancur Cesar , Martínez Yordan
Published: June 2023

This study aimed to investigate the effects of oral administration with L. plantarum CAM6 on the hematological profile, relative weight of digestive organs, and cecal traits in growing pigs. A total of 36 castrated male pigs [(Landrace × Pietrain) × Duroc] aged 49 to 139 days old were randomly assigned to 3 experimental groups with 12 animals per treatment. The treatments included a control diet without additives (CTRL), a positive control with subtherapeutic antibiotics (TRT1), and CTRL supplemented with 5 mL Lactobacillus plantarum CAM6 preparation providing 109 CFU/pig/day (TRT2). The TRT2 group showed a higher (p ≤ 0.05) small intestine length and the cecum relative weight compared to the CTRL group. Moreover, L. plantarum CAM6 supplementation promoted (p ≤ 0.05) increased thickness of the muscular and mucosal layers, as well as enhanced depth and width of the cecal crypts. The TRT2 group also showed well-defined crypts without lesions, while the CTRL and TRT1 groups exhibited congestion, lymphocytic infiltration in the crypt, and intestinal-associated lymphoid tissue atrophy, respectively. Additionally, TRT2 stimulated (p ≤ 0.05) the growth of the autochthonous cecal microbiota compared to other experimental groups. Overall, the results indicate that oral administration of L. plantarum CAM6 improved intestinal health and enhanced the growth of autochthonous cecal lactic acid bacteria and had no impact on the complete blood count in growing pigs.
In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis
Authors: Lillo Edoardo , Pellegrini Francesco , Rizzo Annalisa , Lanave Gianvito , Zizzadoro Claudia , Cicirelli Vincenzo , Catella Cristiana , Losurdo Michele , Martella Vito , Tempesta Maria , Camero Michele
Published: June 2023

Alphaherpesviruses cause genital lesions and reproductive failure in both humans and animals. Their control is mainly based on prevention using hygienic prophylactic measures due to the absence of vaccines and limitations of antiviral drug therapy. Ozone is an oxidating gas showing a strong microbicidal activity on bacteria, fungi, viruses, and protozoa. The present study assessed the in vitro virucidal and antiviral activity of ozone against caprine herpesvirus type 1 (CpHV-1). The virucidal activity of a gaseous mixture containing O3 at 20 and 50 μg/mL was assessed against the virus at different contact times (30 s, 60 s, 90 s, 120 s, 180 s, and 300 s). Antiviral activity of a gaseous mixture containing O3 at 20 and 50 μg/mL was evaluated against the virus after 30 s and 60 s. Ozone displayed significant virucidal activity when used at all the tested concentrations whilst significant antiviral activity was observed using ozone at 50 μg/mL. The gaseous mixture, tested in the present study, showed virucidal and antiviral activity against CpHV-1 in a dose- and time contact-dependent fashion. Ozone therapy could be evaluated in vivo for the treatment of CpHV-1-induced genital lesions in goats using topical applications.
Longitudinal Changes in Milk Microorganisms in the First Two Months of Lactation of Primiparous and Multiparous Cows
Authors: Zhu Huan , Miao Renfang , Tao Xinxu , Wu Jianhao , Liu Licheng , Qu Jiachen , Liu Hongzhi , Sun Yanting , Li Lingyan , Qu Yongli
Published: June 2023

The present experiment was carried out to analyze the longitudinal changes in milk microorganisms. For this purpose, milk samples were collected from 12 healthy cows (n = 96; six primiparous cows and six multiparous cows) at eight different time points. The characteristics and variations in microbial composition were analyzed by 16S rRNA gene high-throughput sequencing. In the primiparous group, higher and more stable alpha diversity was observed in transitional and mature milk compared with the colostrum, with no significant difference in alpha diversity at each time point in the multiparous group. Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteriota were the most dominant phyla, and Pseudomonas, UCG-005, Acinetobacter, Vibrio, Lactobacillus, Bacteroides, Serratia, Staphylococcus, and Glutamicibacter were the most dominant genera in both primiparous and multiparous cow milk. Some typically gut-associated microbes, such as Bacteroides, UCG-005, and Rikenellaceae_RC9_gut_group, etc., were enriched in the two groups. Biomarker taxa with the day in time (DIM) were identified by a random forest algorithm, with Staphylococcus showing the highest degree of interpretation, and the difference in milk microbiota between the two groups was mainly reflected in 0 d–15 d. Additionally, network analysis suggested that there were bacteria associated with the total protein content in milk. Collectively, our results disclosed the longitudinal changes in the milk microbiota of primiparous and multiparous cows, providing further evidence in dairy microbiology.
Means of Livelihood, Clean Environment to Women Empowerment: The Multi-Faceted Role of Donkeys
Authors: Ravichandran Thanammal , Perumal Ramesh Kumar , Vijayalakshmy Kennady , Raw Zoe , Cooke Fiona , Baltenweck Isabelle , Rahman Habibar
Published: June 2023

Despite the substantial contribution donkeys make to the livelihood of the world’s poorest populations, the existence of donkeys has received little notice worldwide. This article reviews the value of donkeys in a variety of sectors, including agriculture, construction industry, and mining, as well as their role in empowering women and achieving sustainable development goals. However, donkeys and mules are not given enough credit or attention in terms of developing strategies regarding their role in reducing poverty. There is a dearth of information and statistics on their impact across industries, the factors contributing to the donkey population dropping, the socioeconomic status of the dependent communities, and related animal and human welfare issues.
Morphometric Characterization of Local Goat Breeds in Two Agroecological Zones of Burkina Faso, West Africa
Authors: Akounda Badjibassa , Ouédraogo Dominique , Soudré Albert , Burger Pamela A. , Rosen Benjamin D. , Van Tassell Curtis P. , Sölkner Johann
Published: June 2023

In Burkina Faso, goats are the second most numerous ruminant livestock population, with almost exclusively indigenous breeds being reared in extensive production systems in various agroecological zones. This study was carried out to understand the morphological variation of local goat breeds in the Sudano-Sahelian and Sudanian agroecological zones. A total of 511 adult female animals belonging to two presumed populations (Mossi breed in Sudano-Sahelian zone and Djallonké breed in Sudanian zone) were sampled and body weight as well as a range of linear body measurements, following FAO guidelines, were recorded. The least squares means of body measurements of indicated that Sudano-Sahelian goats have significantly (p < 0.001) larger body measurements than Sudanian goats. Furthermore, relative high variability of the two populations in morphometric traits was observed. Principal Component Analysis (PCA) suggested structure between Mossi breed on one side and Djallonké on the other side, but no strict separation was observed, suggesting that gene flow is occurring among the different populations. A dispersion map with four clusters was built based on the first two factors. The least square means of body measurements ranked the four groups from small to large body size, namely Djallonké, Mossi × Djallonké, Mossi, and Sahelian × Mossi. Gene flow from Sahelian goat into other populations of the country, based on migration of the Fulani ethnic group from the Sahel into areas with Mossi and Djallonké breeds, could explain this configuration and confirms the continuous erosion of genetic identity of these two local breeds. The sustainable use of these adapted local goat genetic resources calls for the promotion of sustainable genetic improvement using participatory breeding approaches.
Down-Syndrome-Related Maternal Dysbiosis Might Be Triggered by Certain Classes of Antibiotics: A New Insight into the Possible Pathomechanisms
Authors: Ternák Gábor , Márovics Gergely , Sümegi Katalin , Bánfai Zsolt , Büki Gergely , Magyari Lili , Szabó András , Melegh Béla
Published: June 2023

Down syndrome (DS) is a leading human genomic abnormality resulting from the trisomy of chromosome 21. The genomic base of the aneuploidy behind this disease is complex, and this complexity poses formidable challenges to understanding the underlying molecular basis. In the spectrum of the classic DS risk factor associations, the role of nutrients, vitamins, and, in general, the foodborne-associated background, as part of the events ultimately leading to chromosome nondisjunction, has long been recognized as a well-established clinical association. The integrity of the microbiome is a basic condition in these events, and the dysbiosis may be associated with secondary health outcomes. The possible association of DS development with maternal gut microbiota should therefore require more attention. We have hypothesized that different classes of antibiotics might promote or inhibit the proliferation of different microbial taxa; and hence, we might find associations between the use of the different classes of antibiotics and the prevalence of DS through the modification of the microbiome. As antibiotics are considered major disruptors of the microbiome, it could be hypothesized that the consumption/exposure of certain classes of antibiotics might be associated with the prevalence of DS in European countries (N = 30). By utilizing three different statistical methods, comparisons have been made between the average yearly antibiotic consumption (1997–2020) and the estimated prevalence of people living with DS for the year 2019 as a percentage of the population in European countries. We have found strong statistical correlations between the consumption of tetracycline (J01A) and the narrow-spectrum, beta-lactamase-resistant penicillin (J01CF) and the prevalence of DS.
Application of a Structured Training Plan on Different-Length Microcycles in Soccer—Internal and External Load Analysis between Training Weeks and Games
Authors: Vardakis Lazaros , Michailidis Yiannis , Topalidis Panagiotis , Zelenitsas Charalambos , Mandroukas Athanasios , Gissis Ioannis , Christoulas Kosmas , Mavrommatis George , Metaxas Thomas
Published: June 2023

The aim of this study was to apply a training plan to four different-length microcycles (MIC) in soccer and analyze (a) the load within the training weeks and (b) the short-term effect on the matches that were played at the end of each microcycle. An intervention training program applied to microcycles of five, six, seven and nine days during two seasons of the Cypriot Fist Division. The GPS technology and subjective (wellness, RPE) assessments were used to monitor the load throughout trainings and games. In weekly external load, there were differences between the four microcycles, with a lower load in MIC5 for all the parameters and higher on MIC9 in the most of them (p < 0.05). In RPE, MIC9 (229 ± 60 arbitrary units (au)) differed significantly from MIC5 (229 ± 60 au, p < 0.001), MIC6 (281 ± 67 au; p < 0.001) and MIC7 (297 ± 48 au, p = 0.009). MIC5 also differed from MIC6 (p = 0.001) and MIC7 (p < 0.001). In the game external load, the only differences found in GDEC2 (game decelerations) were between MIC7 (68 ± 10 number (n)), MIC5 (61 ± 11 n, p = 0.035) and MIC6 (60 ± 10 n, p = 0.002); in GSPEF (game speed efforts), between MIC5 (40 ± 17 n), MIC7 (48 ± 14 n, p = 0.004) and MIC9 (48 ± 16 n, p < 0.001) and between MIC6 (41 ± 14 n), MIC7 (p = 0.009), and MIC9 (p = 0.009); in GMPW5 (game metabolic power efforts), between MIC7 (1307 ± 271 n), MIC5 (1201 ± 340 n, p = 0.035) and MIC6 (1178 ± 261 n, p = 0.001). No differences were found for wellness and perceived exertion. It is important for performance coaches to adapt the training load to the length of the microcycle, applying a lower load to short training weeks and manage the load fluctuation on long training weeks in terms of volume, intensity and recovery. In our study, the results confirmed that this strategy could result in similar performance in the games, regardless of microcycle length.
Wheat Bread Enriched with Black Chokeberry (Aronia melanocarpa L.) Pomace: Physicochemical Properties and Sensory Evaluation
Authors: Cacak-Pietrzak Grażyna , Dziki Dariusz , Gawlik-Dziki Urszula , Parol-Nadłonek Natalia , Kalisz Stanisław , Krajewska Anna , Stępniewska Sylwia
Published: June 2023

Fruit pomace is a highly valuable byproduct from a nutritional standpoint. The aim of this study was to determine the physicochemical and sensory properties of wheat bread enriched with freeze-dried and powdered chokeberry pomace in amounts of 1, 2, 3, 4, 5, and 6% relative to the flour weight. The influence of pomace addition on the physical properties of the wheat dough was analyzed, and the physicochemical properties and consumer acceptance of the chokeberry-pomace-enriched bread were determined. Based on the obtained research results, it was shown that the addition of pomace increased the water absorption of the flour but caused a decrease in stability and weakening of the dough, resulting in increased softening. Consequently, the volume of the bread decreased, and the crumb hardness increased. Furthermore, the addition of pomace significantly reduced the brightness and yellowness of the bread crumbs, while making them more red. Importantly, compared to the control bread, the pomace-enriched bread was characterized by higher contents of minerals, dietary fiber, phenolic compounds, and higher antioxidant activity. Sensory evaluation showed that the addition of freeze-dried chokeberry pomace to wheat bread should not exceed 3% in relation to the weight of the flour used. Additionally, a 3% addition of chokeberry pomace increased the dietary fiber content by 80.9%, ash content by 2.6%, fat content by 26.5%, and total phenolics content by 272%. It decreased the protein content by 1.2%, and reduced the carbohydrate content by 4% compared to the control sample.