bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring Behavioral Creativity of a Proactive Robot
Authors: Buyukgoz Sera , Pandey Amit Kumar , Chamoux Marine , Chetouani Mohamed
Published: December 2021

Creativity, in one sense, can be seen as an effort or action to bring novelty. Following this, we explore how a robot can be creative by bringing novelty in a human–robot interaction (HRI) scenario. Studies suggest that proactivity is closely linked with creativity. Proactivity can be defined as acting or interacting by anticipating future needs or actions. This study aims to explore the effect of proactive behavior and the relation of such behaviors to the two aspects of creativity: 1) the perceived creativity observed by the user in the robot’s proactive behavior and 2) creativity of the user by assessing how creativity in HRI can be shaped or influenced by proactivity. We do so by conducting an experimental study, where the robot tries to support the user on the completion of the task regardless of the end result being novel or not and does so by exhibiting anticipatory proactive behaviors. In our study, the robot instantiates a set of verbal communications as proactive robot behavior. To our knowledge, the study is among the first to establish and investigate the relationship between creativity and proactivity in the HRI context, based on user studies. The initial results have indicated a relationship between observed proactivity, creativity, and task achievement. It also provides valuable pointers for further investigation in this domain.
Automating Endoscope Motion in Robotic Surgery: A Usability Study on da Vinci-Assisted Ex Vivo Neobladder Reconstruction
Authors: Da Col Tommaso , Caccianiga Guido , Catellani Michele , Mariani Andrea , Ferro Matteo , Cordima Giovanni , De Momi Elena , Ferrigno Giancarlo , de Cobelli Ottavio
Published: December 2021

Robots for minimally invasive surgery introduce many advantages, but still require the surgeon to alternatively control the surgical instruments and the endoscope. This work aims at providing autonomous navigation of the endoscope during a surgical procedure. The autonomous endoscope motion was based on kinematic tracking of the surgical instruments and integrated with the da Vinci Research Kit. A preclinical usability study was conducted by 10 urologists. They carried out an ex vivo orthotopic neobladder reconstruction twice, using both traditional and autonomous endoscope control. The usability of the system was tested by asking participants to fill standard system usability scales. Moreover, the effectiveness of the method was assessed by analyzing the total procedure time and the time spent with the instruments out of the field of view. The average system usability score overcame the threshold usually identified as the limit to assess good usability (average score = 73.25 > 68). The average total procedure time with the autonomous endoscope navigation was comparable with the classic control (p = 0.85 > 0.05), yet it significantly reduced the time out of the field of view (p = 0.022 < 0.05). Based on our findings, the autonomous endoscope improves the usability of the surgical system, and it has the potential to be an additional and customizable tool for the surgeon that can always take control of the endoscope or leave it to move autonomously.
Morphological Computation in Plant Seeds for a New Generation of Self-Burial and Flying Soft Robots
Authors: Mazzolai Barbara , Mariani Stefano , Ronzan Marilena , Cecchini Luca , Fiorello Isabella , Cikalleshi Kliton , Margheri Laura
Published: December 2021

Plants have evolved different mechanisms to disperse from parent plants and improve germination to sustain their survival. The study of seed dispersal mechanisms, with the related structural and functional characteristics, is an active research topic for ecology, plant diversity, climate change, as well as for its relevance for material science and engineering. The natural mechanisms of seed dispersal show a rich source of robust, highly adaptive, mass and energy efficient mechanisms for optimized passive flying, landing, crawling and drilling. The secret of seeds mobility is embodied in the structural features and anatomical characteristics of their tissues, which are designed to be selectively responsive to changes in the environmental conditions, and which make seeds one of the most fascinating examples of morphological computation in Nature. Particularly clever for their spatial mobility performance, are those seeds that use their morphology and structural characteristics to be carried by the wind and dispersed over great distances (i.e. “winged” and “parachute” seeds), and seeds able to move and penetrate in soil with a self-burial mechanism driven by their hygromorphic properties and morphological features. By looking at their motion mechanisms, new design principles can be extracted and used as inspiration for smart artificial systems endowed with embodied intelligence. This mini-review systematically collects, for the first time together, the morphological, structural, biomechanical and aerodynamic information from selected plant seeds relevant to take inspiration for engineering design of soft robots, and discusses potential future developments in the field across material science, plant biology, robotics and embodied intelligence.
Pooled Prevalence of Adverse Pregnancy and Neonatal Outcomes in Malawi, South Africa, Uganda, and Zimbabwe: Results From a Systematic Review and Meta-Analyses to Inform Trials of Novel HIV Prevention Interventions During Pregnancy
Authors: Lokken Erica M. , Mathur Anya , Bunge Katherine E. , Fairlie Lee , Makanani Bonus , Beigi Richard , Noguchi Lisa , Balkus Jennifer E.
Published: December 2021

Background: Robust data summarizing the prevalence of pregnancy and neonatal outcomes in low- and middle-income countries are critically important for studies evaluating investigational products for HIV prevention and treatment in pregnant and breastfeeding women. In preparation for studies evaluating the safety of the dapivirine vaginal ring for HIV prevention in pregnancy, we conducted a systematic literature review and meta-analyses to summarize the prevalence of pregnancy and neonatal outcomes in Malawi, South Africa, Uganda, and Zimbabwe.

Methods: Ten individual systematic literature reviews were conducted to identify manuscripts presenting prevalence data for 12 pregnancy and neonatal outcomes [pregnancy loss, stillbirth, preterm birth, low birthweight (LBW), neonatal mortality, congenital anomaly, chorioamnionitis, postpartum endometritis, postpartum hemorrhage, gestational hypertension, preeclampsia/eclampsia, and preterm premature rupture of membranes (PPROM)]. Studies included in the meta-analyses were published between January 1, 1998, and July 11, 2018, provided numerator and denominator data to support prevalence estimation, and included women of any HIV serostatus. Random-effects meta-analyses were conducted to estimate the pooled prevalence and 95% confidence interval (CI) for each outcome overall, by country, and by HIV status.

Results: A total of 152 manuscripts were included across the 12 outcomes. Overall, the frequency of stillbirth (n = 75 estimates), LBW (n = 68), and preterm birth (n = 67) were the most often reported. However, fewer than 10 total manuscripts reported prevalence estimates for chorioamnionitis, endometritis, or PPROM. The outcomes with the highest pooled prevalence were preterm birth (12.7%, 95%CI 11.2–14.3), LBW (11.7%, 95%CI 10.6–12.9), and gestational hypertension (11.4%, 95%CI 7.8–15.7). Among the outcomes with the lowest pooled prevalence estimates were neonatal mortality (1.7%, 95%CI 1.4–2.1), pregnancy loss [1.9%, 95%CI 1.1–2.8, predominately studies (23/29) assessing losses occurring after the first trimester], PPROM (2.2%, 95%CI 1.5–3.2), and stillbirth (2.5%, 95%CI 2.2–2.7).

Conclusions: Although this review identified numerous prevalence estimates for some outcomes, data were lacking for other important pregnancy-related conditions. Additional research in pregnant populations is needed for a thorough evaluation of investigational products, including for HIV prevention and treatment, and to inform better estimates of the burden of adverse pregnancy outcomes globally.
Hormonal Approach for Postmenopausal Vulvovaginal Atrophy
Authors: Costa Ana Paula Ferreira , Sarmento Ayane Cristine Alves , Vieira-Baptista Pedro , Eleutério José , Cobucci Ricardo Ney , Gonçalves Ana Katherine
Published: December 2021

Menopause is a physiological and progressive phenomenon secondary to decreased ovarian follicular reserve that significantly affects the genital tract. Although postmenopausal vulvovaginal atrophy primarily affects postmenopausal women, it is also seen in premenopausal women. The hypoestrogenic condition results in hormonal and anatomical changes, with the main symptoms, are dryness, burning and genital irritation, decreased lubrication, urinary urgency, dysuria, and recurrent urinary tract infections. This review aims to update hormone therapy for urogenital atrophy, both local and systemic, and discusses the importance of understanding and the need for active treatment of this condition. The main therapeutic objective is the relief of symptoms, and hormonal therapy (HT) is still the most effective choice for treating clinical manifestations, despite the side effects of its use. HT should be used in an individualized way to the needs of the women and appropriate to the stage in which she is menopausal, perimenopausal, or after menopause.
The Impact of Variable Renewable Energy Penetration on Wholesale Electricity Prices in Japan Between FY 2016 and 2019
Authors: Sakaguchi Makishi , Fujii Hidemichi
Published: December 2021

The merit order effect (MOE), which renewable energy sources can decrease wholesale electricity prices, plays an important role in establishing low-carbon societies. After the liberalization of the electricity market, the trade volume of the Japan Electric Power Exchange (JEPX) day-ahead spot market drastically increased between 2016 and 2019; however, price spikes still occur often. Ordinary least squares and quantile regression analyses were applied in this study to investigate how wind and solar photovoltaics (PV) energy generation affect the JEPX day-ahead spot price by time, price range, and area, and we concluded that the MOE of wind increased between 2016 and 2019 while that of PV decreased during this time. In regard to the high price ranges, although wind generation is not significant in terms of reducing price spikes, PV had this effect in 2016 and 2017 but not during the other years covered. The study area was divided into four regions, and each area followed trends that were different from those of the national analysis. Overall, the key finding of our study is that wind power has more potential to reduce electricity prices than PV.
Challenges to the Use of a Base of Fresh Water in Groundwater Management: Total Dissolved Solids vs. Depth Across California
Authors: Warrack Jessica , Kang Mary
Published: December 2021

According to the California Sustainable Groundwater Management Act, the bottom of a basin, and subsequently the depth to which groundwater is managed, can be defined through physical or geochemical qualities of the aquifer. Total dissolved solids (TDS) concentrations are most frequently used to define the basin bottom. However, upper limits in TDS concentrations for “fresh” and “useable” groundwater can range from 1,000 to 10,000 mg/L. To evaluate the applicability of using TDS concentrations to delineate depths subject to sustainable groundwater management, we analyze 216,754 TDS measurements throughout the state of California. We find major challenges to reasonably estimating the BFW with our dataset in 73% of California due to data insufficiencies or complexity introduced by non-montonic TDS-depth relationships. We estimate the BFW in 22% of the Central Valley, a key agricultural region with large groundwater demands and many critically overdrafted groundwater subbasins. Using a TDS limit of 3,000 mg/L, where possible, we estimate the shallowest BFW in the Central Valley to be 155 m below ground surface and the deepest BFW to be 589 m below ground surface. We find that the base of brackish water (TDS < 10,000 mg/L) can extend more than 500 m deeper than the BFW in 78% of the Central Valley where we are able to estimate the BFW, which corresponds to 2% of California. There is a need to evaluate alternative strategies for defining groundwater subject to sustainable management, which likely involves additional measurements and analysis to better characterize groundwater quality with depth throughout California.
Dual Inoculation With Arbuscular Mycorrhizal Fungi and Phosphorus Solubilizing Fungi Synergistically Enhances the Mobilization and Plant Uptake of Phosphorus From Meat and Bone Meal
Authors: Jatana Bhupinder Singh , Kitchens Christopher , Ray Christopher , Gerard Patrick , Tharayil Nishanth
Published: December 2021

Phosphorus (P) is the second most important mineral nutrient for plant growth and plays a vital role in maintaining global food security. The natural phosphorus reserves [phosphate rock (PR)] are declining at an unprecedented rate, which will threaten the sustainable food supply in near future. Rendered animal byproducts such as meat and bone meal (MBM), could serve as a sustainable alternative to meet crop phosphorus demand. Even though nitrogen (N) from MBM is readily mineralized within a few days, >75% of the P in MBM is present as calcium phosphate that is sparingly available to plants. Thus, application of MBM with the aim of meeting crop N demand could result in buildup of P reserves in soil, which necessitates the need to improve the P mobilization from MBM to achieve higher plant P use efficiency. Here, we tested the potential of two microbial inoculum-arbuscular mycorrhizal fungi (AMF) and P solubilizing fungi (Penicillium bilaiae), in improving the mobilization of P from MBM and the subsequent P uptake by maize (Zea mays). Compared to the non-inoculated MBM control, the application of P. bilaiae increased the P mobilization from MBM by more than two-fold and decreased the content of calcium bound P in the soil by 26%. However, despite this mobilization, P. bilaiae did not increase the tissue content of P in maize. On the other hand, AMF inoculation with MBM increased the plant root, shoot biomass, and plant P uptake as compared to non-inoculated control, but did not decrease the calcium bound P fraction of the soil, indicating there was limited P mobilization. The simultaneous application of both AMF and P. bilaiae in association with MBM resulted in the highest tissue P uptake of maize with a concomitant decrease in the calcium bound P in the soil, indicating the complementary functional traits of AMF and P. bilaiae in plant P nutrition from MBM. Arbuscular mycorrhizal fungi inoculation with MBM also increased the plant photosynthesis rate (27%) and root phosphomonoesterase activity (40%), which signifies the AMF associated regulation of plant physiology. Collectively, our results demonstrate that P mobilization and uptake efficiency from MBM could be improved with the combined use of arbuscular mycorrhizal fungi and P. bilaiae.
Establishing the Role of Elbow Muscles by Evaluating Muscle Activation and Co-contraction Levels at Maximal External Rotation in Fastball Pitching
Authors: van Trigt Bart , Galjee Eva , Hoozemans Marco J. M. , van der Helm Frans C. T. , Veeger DirkJan H. E. J.
Published: December 2021

Background: Baseball pitching is associated with a high prevalence of ulnar collateral ligament injuries, potentially due to the high external valgus load on the medial side of the elbow at the instant of maximal shoulder external rotation (MER). In-vitro studies show that external valgus torque is resisted by the ulnar collateral ligament but could also be compensated by elbow muscles. As the potential active contribution of these muscles in counteracting external valgus load during baseball pitching is unknown, the aim of this study is to determine whether and to what extent the elbow muscles are active at and around MER during a fastball pitch in baseball.

Methods: Eleven uninjured pitchers threw 15 fastball pitches. Surface electromyography of six muscles crossing the elbow were measured at 2000 Hz. Electromyography signals were normalized to maximal activity values. Co-contraction index (CCI) was calculated between two pairs of the flexor and extensor elbow muscles. Confidence intervals were calculated at the instant of MER. Four ranges of muscle activity were considered; 0–20% was considered low; 21–40% moderate; 41–60% high and over 60% as very high. To determine MER, the pitching motion was captured with a highspeed camera at 240 Hz.

Results: The flexor pronator mass, pronator teres, triceps brachii, biceps brachii, extensor supinator mass and anconeus show moderate activity at MER. Considerable variation between participants was found in all muscles. The CCI revealed co-contraction of the two flexor-extensor muscle pairs at MER.

Interpretation: The muscle activation of the flexor and pronator muscles at MER indicates a direct contribution of forearm muscles crossing the medial side of the elbow in counteracting the external valgus load during fastball pitching. The activation of both flexor and extensor muscles indicates an in-direct contributory effect as the combined activity of these muscles counteract opening of the humeroulnar joint space. We believe that active muscular contributions counteracting the elbow valgus torque can be presumed to relieve the ulnar collateral ligament from maximal stress and are thus of importance in injury risk assessment in fastball pitching in baseball.
Bridge Over Troubled Water: Shared Understanding Bridges Individual and Collective Resources in Developing Team Resilience in Professional Football
Authors: Grinde Ole Erik
Published: December 2021

This study explored how coaches facilitate coordinated activities through shared understanding in the processes of team resilience development. Constructs of shared information that underpin synchronised actions and behaviour in a team are investigated through individual experiences with a dialogic “we” perspective of appropriating and handling challenging situations. Interactional key elements underpin coordinated task actions within the team. Experiences of both players and coaches are investigated through semi-structured interviews and complementary texts such as an observation log and coach-meeting reports, originating as part of an action research process in the team environment. The interaction model is developed in the exploratory journey during the season with the team. The model suggests key strategic elements that help to bridge shared appropriation of information to strengthen role interactions between team members handling challenging situations. Coaching practise, which connects the interaction model to different team resources of coordinating activities in the development process, still needs to be explored from different contextual perspectives and environments, within the development of team resilience.
A Framework for Assessing Food System Governance in Six Urban and Peri-Urban Regions in Sub-saharan Africa
Authors: Chen Qiuzhen , Knickel Karlheinz , Tesfai Mehreteab , Sumelius John , Turinawe Alice , Isoto Rosemary Emegu , Medyna Galyna
Published: December 2021

An important goal across Sub-Saharan Africa (SSA), and globally, is to foster a healthy nutrition. A strengthening of the diversity, sustainability, resilience and connectivity of food systems is increasingly seen as a key leverage point. Governance arrangements play a central role in connecting sustainable, resilient farming with healthy nutrition. In this article, we elaborate a framework for assessing, monitoring and improving the governance of food systems. Our focus is on food chains in six peri-urban and urban regions in SSA. A literature review on food chain governance and a mapping of current agri-food chains in the six regions provide the basis for the elaboration of an indicator-based assessment framework. The framework is adapted to the specific conditions of SSA and related goals. The assessment framework is then used to identify the challenges and opportunities in food chain governance in the six regions. The first testing of the framework indicates that the approach can help to identify disconnects, conflicting goals and tensions in food systems, and to formulate strategies for empowering agri-food chain actors in transitioning toward more efficient, equitable and sustainable agri-food systems. The article is concluded with a brief reflection on the strengths and weaknesses of the framework and suggests further testing and refinement.
Learning Curve of Ex Vivo Liver Resection and Autotransplantation in Treating End-Stage Hepatic Alveolar Echinococcosis: A RA-CUSUM Analysis
Authors: Qiu Yiwen , Yang Xianwei , Wang Tao , Shen Shu , Yang Yi , Huang Bin , Wang Wentao
Published: December 2021

Background: This retrospective study aimed to evaluate the safety and learning curve of ex vivo liver resection and autotransplantation (ELRA).

Methods: A total of 102 consecutive end-stage HAE patients who underwent ELRA between 2014 and 2020 in West China Hospital were enrolled. The primary endpoint was major postoperative complications (comprehensive complication index, CCI > 26). The ELRA learning curve was evaluated using risk-adjusted cumulative sum (RA-CUSUM) methods. The learning phases were determined based on RA-CUSUM analysis and tested for their association with intra- and post-operative endpoints.

Results: The median surgery time was 738 (659–818) min, with a median blood loss of 2,250 (1,600–3,000) ml. The overall incidence of major morbidity was 38.24% (39/102). Risk-adjusted cumulative sum analysis demonstrated a learning curve of 53 ELRAs for major postoperative complications. The learning phase showed a significant association with the hemodynamic unstable time (HR −30.29, 95% CI −43.32, −17.25, P < 0.0001), reimplantation time (HR −13.92, 95% CI −23.17, −4.67, P = 0.004), total postoperative stay (HR −6.87, 95% CI −11.33, −2.41, P = 0.0033), and postoperative major morbidity (HR 0.25, 95% CI 0.09, 0.68, p = 0.007) when adjusted for age, disease course, liver function, and remote metastasis.

Discussion:Ex vivo liver resection and autotransplantation is feasible and safe with a learning curve of 53 cases for major postoperative complications.
Descriptive and Multivariate Analysis of the Pig Sector in North Macedonia and Its Implications for African Swine Fever Transmission
Authors: O'Hara Kathleen C. , Beltrán-Alcrudo Daniel , Hovari Mark , Tabakovski Blagojco , Martínez-López Beatriz
Published: December 2021

North Macedonia, a country in the Balkan region of Europe, is currently bordered to the north and east by countries with active African swine fever (ASF) outbreaks. The predominantly traditional backyard pig farming sector in this country is under imminent threat of disease incursion. The characteristics and practices of such sectors have rarely been described, and thus the implications for these factors on disease introduction and spread are poorly understood. Using a semi-structured questionnaire, 457 pig producers were interviewed, providing information on 77.7% of the pig population in North Macedonia. In addition, a pilot study of 25 pig producers in Kosovo was performed. This study aimed to provide a detailed description of the North Macedonian pig sector, to make comparisons with nearby Kosovo, and to identify areas with high-risk practices for targeted mitigation. Descriptive data were summarized. Results of the questionnaire were used to identify farm-level risk factors for disease introduction. These factors were used in the calculation of a biosecurity risk score. Kernel density estimation methods were used to generate density maps highlighting areas where the risk of disease introduction was particularly concentrated. Multiple correspondence analysis with hierarchical clustering on principal components was used to explore patterns in farm practices. Results show that farms were predominantly small-scale with high rates of turnover. Pig movement was predominantly local. The highest biosecurity risk scores were localized in the eastern regions of North Macedonia, concerningly the same regions with the highest frequency of wild boar sightings. Veterinarians were highly regarded, regularly utilized, and trusted sources of information. Practices that should be targeted for improvement include isolation of new pigs, and consistent application of basic sanitary practices including washing hands, use of disinfection mats, and separation of clean and dirty areas. This study provides the most complete description of the North Macedonian pig sector currently available. It also identifies regions and practices that could be targeted to mitigate the risk of disease incursion and spread. These results represent the first steps to quantify biosecurity gaps and high-risk behaviors in North Macedonia, providing baseline information to design risk-based, more cost-effective, prevention, surveillance, and control strategies.
Linguistic Validation of the Canine Brief Pain Inventory (CBPI) for Global Use
Authors: Wells Jane R. , Young Alyson L. , Crane Alexandra , Moyaert Hilde , Michels Gina , Wright Andrea
Published: December 2021

A valid and reliable quantitative measure of chronic pain is essential for developing and evaluating interventions that aim to treat pain. In dogs, the Canine Brief Pain Inventory (CBPI) was originally adapted from a human measure, the Brief Pain Inventory, to assess owner-perceived pain and the impact of such pain on a dog's daily functioning. To be reliable and valid, data collected using a translated instrument should have evidence it is an accurate representation of the original instrument and is culturally appropriate for use in the intended context. To achieve this, instruments should undergo a rigorous translation process and be debriefed in the intended population of use. The CBPI is widely accepted and has been fully validated for use in US-English, Swedish, Italian, and French (France); further translation and validation of the CBPI is required to increase access to and use in other languages and countries. The objective of this study was to linguistically validate the CBPI for global use (Australia, China, Germany, Hungary, Ireland, Japan, Netherlands and Portugal). In cognitive debriefing with a representative sample of dog owners in the target countries it was confirmed that the translations of the CBPI adequately convey the concepts in the original US-English version and that items are easily understood by dog owners. The results of the linguistic validation process thus produced measures that are conceptually equivalent to the original US-English-language CBPI and are culturally appropriate for use in the target countries.
Case Report: Non-traumatic Unilateral Forelimb Arterial Thrombosis Associated With Hyperadrenocorticism in a Dog
Authors: Eom Tae-Yoon , Choi Ju-Won , Yoon Kyong-Ah , Jeong Soon-Wuk , Kim Jung-Hyun
Published: December 2021

A 16-year-old spayed female Pomeranian dog was presented to the hospital with an acute onset of pain and non-weight-bearing lameness in the right forelimb. On physical examination, knuckling, coolness, pain, and cyanosis were observed in the affected forelimb. Peripheral blood glucose concentration and body surface temperature differed between the right and left forelimbs. Hypercoagulable thromboelastographic results and increased D-dimer levels were suggestive of thrombus. Accordingly, recombinant tissue plasminogen activator (rtPA) was administered intravenously. Prompt clinical improvements (including restored warmth of the affected limb) occurred, and rtPA was discontinued after two shots administered 2 h apart owing to concerns of bleeding side effects. The dog was discharged 6 days after admission, and outpatient treatment with clopidogrel was continued for the prevention of re-thrombosis. Following patient stabilization, further examinations for underlying diseases of hypercoagulability were conducted; hyperadrenocorticism (HAC) was diagnosed, and oral trilostane therapy was thus administered. Eight weeks later, the patient regained normal mobility. Finally, in the present canine patient with arterial thrombosis, thrombolysis with rtPA successfully improved clinical symptoms and the following administration of clopidogrel inhibited the formation of additional thrombus.
Performance of tasks related to state security in the European Union conditions by the President of the Republic of Poland
Authors: Tomaszewski Waldemar
Published: December 2021

The subject of the article is to consider the theoretical and practical possibilities of the President of the Republic of Poland to perform his constitutional tasks in the area of state security. The presented analysis takes into account the legal and institutional conditions resulting from Poland’s membership in the European Union.
Free-Vertex Tetrahedral Finite-Element Representation and Its Use for Estimating Density Distribution of Irregularly-Shaped Asteroids
Authors: Yin Weidong , Shu Leizheng , Yu Yang , Shi Yu
Published: December 2021

In this article, we present a free-vertex tetrahedral finite-element representation of irregularly shaped small bodies, which provides an alternative solution for estimating asteroid density distribution. We derived the transformations between gravitational potentials expressed by the free-vertex tetrahedral finite elements and the spherical harmonic functions. Inversely, the density of each free-vertex tetrahedral finite element can be estimated via the least-squares method, assuming a spherical harmonic gravitational function is present. The proposed solution is illustrated by modeling gravitational potential and estimating the density distribution of the simulated asteroid 216 Kleopatra.
Lead Bioaccumulation and Translocation in Herbaceous Plants Grown in Urban and Peri-Urban Soil and the Potential Human Health Risk
Authors: Baldi Ada , Cecchi Stefano , Grassi Chiara , Zanchi Camillo A. , Orlandini Simone , Napoli Marco
Published: December 2021

Lead (Pb) contamination risks to crops grown in urban and peri-urban soils is a great concern that should be better evaluated to define the Pb maximum levels in soils for safe cultivation and to identify suitable strategies to remediate Pb polluted urban soils. The objective of this work was to evaluate the potential risk for human health from the ingestion of the edible portions of barley, castor bean, common bean, Indian mustard, sorghum, spinach, and tomato grown in an unpolluted soil (initial Pb content 32.6 mg kg−1) spiked with 0, 300, 650, 1000 mg Pb kg−1, respectively. The potential possibility of using these plants to phyto-remediate the soil of Pb was also assessed. Pot trials were conducted for two years (2008 and 2009). Results highlighted that all the investigated species were able to attain growth to maturity in high Pb spiked soil, although Pb influenced dry matter accumulation. Even in soils with low Pb concentrations, Pb accumulated the edible parts. Noteworthy, even in untreated control soils, all tested species revealed a Pb concentration in the edible parts that was higher than the safe limit set by FAO/WHO. None of the investigated species were considered Pb hyperaccumulators, but all were shown to be potentially suitable for phyto-stabilization.
Comparative Study of Sample-Preparation Techniques for Quantitative Analysis of the Mineral Composition of Humic Substances by Inductively Coupled Plasma Atomic Emission Spectroscopy
Authors: Karpukhina Evgeniya A. , Vlasova Elena A. , Volkov Dmitry S. , Proskurnin Mikhail A.
Published: December 2021

Five sample-preparation techniques were compared to quantify 31 elements in coal humic substances (HS) by ICP–AES from the viewpoints of complete isolation and speciation of elements. They include, for bulk composition, preparation of an aqueous colloidal HS solution followed by direct injection of the sample without decomposition and ashing followed by metaborate fusion; for element speciation, preparation of an aqueous colloidal HS solution followed by centrifugation and direct analysis without decomposition for water-soluble species; treatment with boiling nitric acid; and microwave-assisted treatment with nitric acid at 250 °C for acid-isolated species. The results of analysis significantly depend on the selected method of sample preparation due to specific features of HS, the simultaneous presence of many inorganic components in wide concentration ranges, and a significant fraction of the organic matrix; therefore, the total mineral composition of HS, both macro- and microcomponents, requires a combination of decomposition methods.
Anisotropic Silver Nanoparticles Gel Exhibits Antibacterial Action and Reduced Scar Formation on Wounds Contaminated with Methicillin-Resistant Staphylococcus pseudintermedius (MRSP) in a Mice Model
Authors: Thammawithan Saengrawee , Srichaiyapol Oranee , Siritongsuk Pawinee , Daduang Sakda , Klaynongsruang Sompong , Prapasarakul Nuvee , Patramanon Rina
Published: December 2021

Staphylococcus pseudintermedius (S. pseudintermedius) infected wounds can cause seriously delayed wound healing processes in animals. Antimicrobial agents that have antimicrobial and wound healing efficacy have become an essential tool for overcoming this problem. In our previous study, anisotropic AgNPs have been reported to have antimicrobial efficiency against animal and human pathogens, and could be suitable as antimicrobial agents for infected wounds. Here, antimicrobial and wound healing activities of anisotropic AgNPs gels were assessed in vivo. BALB/cAJcl mice wounds were infected by Methicillin-resistant Staphylococcus pseudintermedius (MRSP). Then, antibacterial and wound healing activities were evaluated by bacterial cell count, wound contraction, digital capture, and histology. The results show that anisotropic AgNPs gels could eliminate all bacterial cell infected wounds within 7 days, the same as povidone iodine. Wound healing activity was evaluated by wound contraction (%). The results showed 100% wound contraction in groups treated with anisotropic AgNPs gels within 14 days that was not significantly different from povidone iodine and control gel without AgNPs. However, the digital capture of wounds on day 4 showed that anisotropic AgNPs gel prevented pus formation and reduced scar appearance within 21 days. The histology results exhibit improved collagen fiber alignment that supports scar disappearance. In conclusion, these results indicate that anisotropic AgNPs gels are suitable for treating infected wounds. The gel is effective in eliminating bacteria that supports the natural process of wound repair and also causes reduced scar formation.
Effects of Eimeria tenella Infection on Key Parameters for Feed Efficiency in Broiler Chickens
Authors: Choi Janghan , Ko Hanseo , Tompkins Yuguo Hou , Teng Po-Yun , Lourenco Jeferson M. , Callaway Todd R. , Kim Woo Kyun
Published: December 2021

The purpose of the study was to investigate effects of different inoculation dosages of E. tenella on growth performance, gastrointestinal permeability, oocyst shedding, intestinal morphology, fecal consistency, ileal apparent digestibility, antioxidant capacity, and cecal VFA profile in broiler chickens. Five different dosages (T0: 0, T1: 6250, T2: 12,500, T3: 25,000, and T4: 50,000) of E. tenella oocysts were inoculated via oral gavage to fourteen-day-old broilers. Inoculation of E. tenella linearly increased FCR (p < 0.05), and feed intake was quadratically increased on 6 days post-infection (dpi; p = 0.08) and 7 dpi (p = 0.09). Cecal lesion score of each treatment was T0: 0; T1: 0.39 ± 0.14; T2: 0.93 ± 0.21; T3: 1.25 ± 0.16; and T4: 1.58 ± 0.2. Cecal total VFA production was linearly reduced due to E. tenella infection on 6 dpi (p < 0.01). E. tenella infection deepened cecal crypts depth on 6 dpi (CD; p < 0.05). Gastrointestinal permeability tended to be linearly increased (p = 0.07). E. tenella infection tended to linearly reduce duodenal VH (p = 0.1) and jejunal VH on 9 dpi (p = 0.09). Different dosages of E. tenella modulated the tendency of fecal moisture content and oocyst shedding. Therefore, E. tenella infection impaired feed efficiency and small intestinal health mainly by reducing cecal VFA production and deepening cecal CD in broilers.
Central Nervous System Delivery of Antibodies and Their Single-Domain Antibodies and Variable Fragment Derivatives with Focus on Intranasal Nose to Brain Administration
Authors: Soleimanizadeh Arghavan , Dinter Heiko , Schindowski Katharina
Published: December 2021

IgG antibodies are some of the most important biopharmaceutical molecules with a high market volume. In spite of the fact that clinical therapies with antibodies are broadly utilized in oncology, immunology and hematology, their delivery strategies and biodistribution need improvement, their limitations being due to their size and poor ability to penetrate into tissues. In view of their small size, there is a rising interest in derivatives, such as single-domain antibodies and single-chain variable fragments, for clinical diagnostic but also therapeutic applications. Smaller antibody formats combine several benefits for clinical applications and can be manufactured at reduced production costs compared with full-length IgGs. Moreover, such formats have a relevant potential for targeted drug delivery that directs drug cargo to a specific tissue or across the blood–brain barrier. In this review, we give an overview of the challenges for antibody drug delivery in general and focus on intranasal delivery to the central nervous system with antibody formats of different sizes.
Nanotechnology as a Novel Approach in Combating Microbes Providing an Alternative to Antibiotics
Authors: Mubeen Bismillah , Ansar Aunza Nayab , Rasool Rabia , Ullah Inam , Imam Syed Sarim , Alshehri Sultan , Ghoneim Mohammed M. , Alzarea Sami I. , Nadeem Muhammad Shahid , Kazmi Imran
Published: December 2021

The emergence of infectious diseases promises to be one of the leading mortality factors in the healthcare sector. Although several drugs are available on the market, newly found microorganisms carrying multidrug resistance (MDR) against which existing drugs cannot function effectively, giving rise to escalated antibiotic dosage therapies and the need to develop novel drugs, which require time, money, and manpower. Thus, the exploitation of antimicrobials has led to the production of MDR bacteria, and their prevalence and growth are a major concern. Novel approaches to prevent antimicrobial drug resistance are in practice. Nanotechnology-based innovation provides physicians and patients the opportunity to overcome the crisis of drug resistance. Nanoparticles have promising potential in the healthcare sector. Recently, nanoparticles have been designed to address pathogenic microorganisms. A multitude of processes that can vary with various traits, including size, morphology, electrical charge, and surface coatings, allow researchers to develop novel composite antimicrobial substances for use in different applications performing antimicrobial activities. The antimicrobial activity of inorganic and carbon-based nanoparticles can be applied to various research, medical, and industrial uses in the future and offer a solution to the crisis of antimicrobial resistance to traditional approaches. Metal-based nanoparticles have also been extensively studied for many biomedical applications. In addition to reduced size and selectivity for bacteria, metal-based nanoparticles have proven effective against pathogens listed as a priority, according to the World Health Organization (WHO). Moreover, antimicrobial studies of nanoparticles were carried out not only in vitro but in vivo as well in order to investigate their efficacy. In addition, nanomaterials provide numerous opportunities for infection prevention, diagnosis, treatment, and biofilm control. This study emphasizes the antimicrobial effects of nanoparticles and contrasts nanoparticles’ with antibiotics’ role in the fight against pathogenic microorganisms. Future prospects revolve around developing new strategies and products to prevent, control, and treat microbial infections in humans and other animals, including viral infections seen in the current pandemic scenarios.
An Orthogonal Wheel Odometer for Positioning in a Relative Coordinate System on a Floating Ground
Authors: Lu Zhiguo , He Guangda , Wang Ruchao , Wang Shixiong , Zhang Yichen , Liu Chong , Chen Ding , Hou Teng
Published: December 2021

This paper introduces a planar positioning sensing system based on orthogonal wheels and encoders for some surfaces that may float (such as ship decks). The positioning sensing system can obtain the desired position and angle information on any such ground that floats. In view of the current method of using the IMU gyroscope for positioning, the odometer data on these floating grounds are not consistent with the real-time data in the world coordinate system. The system takes advantage of the characteristic of the orthogonal wheel, using four vertical omnidirectional wheels and encoders to position on the floating ground. We design a new structure and obtain the position and angle information of a mobile robot by solving the encoder installed on four sets of omnidirectional wheels. Each orthogonal wheel is provided with a sliding mechanism. This is a good solution to the problem of irregular motion of the system facing the floating grounds. In the experiment, it is found that under the condition that the parameters of the four omnidirectional wheels are obtained by the encoder, the influence of the angle change of the robot in the world coordinate system caused by the flotation of the ground can be ignored, and the position and pose of the robot on the fluctuating ground can be well obtained. Regardless of straight or curved motion, the error can reach the centimeter level. In the mobile floating platform experiment, the maximum error of irregular movement process is 2.43 (±0.075) cm and the RMSE is 1.51 cm.
A Multilayer CARU Framework to Obtain Probability Distribution for Paragraph-Based Sentiment Analysis
Authors: Ke Wei , Chan Ka-Hou
Published: December 2021

Paragraph-based datasets are hard to analyze by a simple RNN, because a long sequence always contains lengthy problems of long-term dependencies. In this work, we propose a Multilayer Content-Adaptive Recurrent Unit (CARU) network for paragraph information extraction. In addition, we present a type of CNN-based model as an extractor to explore and capture useful features in the hidden state, which represent the content of the entire paragraph. In particular, we introduce the Chebyshev pooling to connect to the end of the CNN-based extractor instead of using the maximum pooling. This can project the features into a probability distribution so as to provide an interpretable evaluation for the final analysis. Experimental results demonstrate the superiority of the proposed approach, being compared to the state-of-the-art models.
Silk Fibroin Scaffolds as Biomaterials for 3D Mesenchymal Stromal Cells Cultures
Authors: Milazzo Luisa , Vulcano Francesca , Macioce Giampiero , Marziali Giovanna , Iosi Francesca , Bertuccini Lucia , Falchi Mario , Rech Francesco , Giampaolo Adele , Pecci Raffaella , Campioni Ilaria , Bedini Rossella
Published: December 2021

Silk fibroin (SF), a protein-based fiber extracted from Bombyx mori cocoons, has recently emerged with great potential for the biomedical field to be used as a biomaterial processable in a variety of formats and applications, due to its natural characteristics. The aims of the present study were to characterize the structural properties of the SF scaffolds, in the format of porous sponges, and to investigate their feasibility to support the adhesion of mesenchymal stromal/stem cells isolated from human Wharton’s jelly of the umbilical cord (WJ-MSC). Adhesion is a prerequisite for using the SF scaffold as biomaterial for supporting three-dimensional (3D) WJ-MSC cultures for several applications. The integration among micro-computed tomography, confocal analysis, and field emission scanning electron microscopy allowed carrying out a deep investigation based on quantitative morphological parameters and qualitative observations at high resolution. High levels of porosity, interconnection, and contact surface–volume ratio confirmed the appropriateness of the designed SF porous scaffolds as supports for cell cultures. WJ-MSC was demonstrated to be capable of adhering to and colonizing the SF scaffold applicable as a 3D cell culture system, of conducting in vitro experiments in a more controlled environment, and possibly of being used in tissue engineering, regenerative medicine, and applications in oncology.
Wind Tunnel Test on Windblown Sand Two-Phase Flow Characteristics in Arid Desert Regions
Authors: Huang Bin , Li Zhengnong , Zhang Zhitian , Zhao Zhefei , Gong Bo
Published: December 2021

Windblown sand two-phase flow characteristics become an essential factor in evaluating the windblown sand load on infrastructures and civil structures. Based on the measured wind characteristics in arid desert regions, windblown sand flow fields with three kinds of sand beds are simulated in the wind tunnel, respectively. The results indicate that the characteristic saltation height of sand particles increases with the wind speed and particle size in the windblown sand flow field. As the sand concentration increases, the wind speed decreases, and the turbulence intensity increases. The concentration, energy, and impact pressure of sand particles increase with increasing wind speed and decrease exponentially with increasing height. At the same wind speed, the concentration, energy, and impact pressure of the coarse sand, fine sand, and mixed sand increases, in turn. Moreover, the variation of kinetic energy with height is similar to that of total energy with height and the proportion of potential energy to total energy is quite small.
High Frequency, High Efficiency, and High Power Density GaN-Based LLC Resonant Converter: State-of-the-Art and Perspectives
Authors: Mortazavizadeh Seyed Abolfazl , Palazzo Simone , Amendola Arturo , De Santis Enzo , Di Ruzza Dario , Panariello Giuseppe , Sanseverino Annunziata , Velardi Francesco , Busatto Giovanni
Published: December 2021

Soft switching for both primary and secondary side devices is available by using LLC converters. This resonant converter is an ideal candidate for today’s high frequency, high efficiency, and high power density applications like adapters, Uninterrupted Power Supplies (UPS), Solid State Transformers (SST), electric vehicle battery chargers, renewable energy systems, servers, and telecom systems. Using Gallium-Nitride (GaN)-based power switches in this converter merits more and more switching frequency, power density, and efficiency. Therefore, the present paper focused on GaN-based LLC resonant converters. The converter structure, operation regions, design steps, and drive system are described precisely. Then its losses are discussed, and the magnets and inductance characteristics are investigated. After that, various interleaved topologies, as a solution to improve power density and decrease current ripples, have been discussed. Also, some challenges and concerns related to GaN-based LLC converters have been reviewed. Commercially available power transistors based on various technologies, i.e., GaN HEMT, Silicon (Si) MOSFET, and Silicon Carbide (SiC) have been compared. Finally, the LLC resonant converter has been simulated by taking advantage of LTspice and GaN HEMT merits, as compared with Si MOSFETs.