bluuebunny/crossref_metadata_2025 huggingface dataset:
Air Traffic Complexity Map Based on Linear Dynamical Systems
Authors: Delahaye Daniel , García Adrían , Lavandier Julien , Chaimatanan Supatcha , Soler Manuel
Published: April 2022

This paper presents a new air traffic complexity metric based on linear dynamical systems, of which the goal is to quantify the intrinsic complexity of a set of aircraft trajectories. Previous works have demonstrated that the structure and organization of air traffic are essential factors in the perception of the complexity of an air traffic situation. Usually, they were not able to explicitly address trajectory pattern organization. The new metric, by identifying the organization properties of trajectories in a traffic pattern, captures some of the key factors involved in ATC complexity. The key idea of this work is to find a linear dynamical system which fits a vector field as closely as possible to the observations given by the aircraft positions and speeds. This approach produces an aggregate complexity metric that enables one to identify high (low) complexity regions of the airspace and compare their relative complexity. The metric is very appropriate to compare different traffic situations for any scale (sector or country) by associating a complexity index to each trajectory sample in the airspace. For instance, to compute the complexity for a sector, one must just sum-up the complexity for trajectory samples intersecting such a sector. This computation can also be extended in the time dimension in order to estimate the average complexity in a given airspace for a period of time.
Case Study for Testing the Validity of NOx-Ozone Algorithmic Climate Change Functions for Optimising Flight Trajectories
Authors: Rao Pratik , Yin Feijia , Grewe Volker , Yamashita Hiroshi , Jöckel Patrick , Matthes Sigrun , Mertens Mariano , Frömming Christine
Published: April 2022

One possibility to reduce the climate impact of aviation is the avoidance of climate-sensitive regions, which is synonymous with climate-optimised flight planning. Those regions can be identified by algorithmic Climate Change Functions (aCCFs) for nitrogen oxides (NOx), water vapour (H2O) as well as contrail cirrus, which provide a measure of climate effects associated with corresponding emissions. In this study, we evaluate the effectiveness of reducing the aviation-induced climate impact via ozone (O3) formation (resulting from NOx emissions), when solely using O3 aCCFs for the aircraft trajectory optimisation strategy. The effectiveness of such a strategy and the associated potential mitigation of climate effects is explored by using the chemistry–climate model EMAC (ECHAM5/MESSy) with various submodels. A summer and winter day, characterised by a large spatial variability of the O3 aCCFs, are selected. A one-day air traffic simulation is performed in the European airspace on those selected days to obtain both cost-optimised and climate-optimised aircraft trajectories, which more specifically minimised a NOx-induced climate effect of O3 (O3 aCCFs). The air traffic is laterally and vertically re-routed separately to enable an evaluation of the influences of the horizontal and vertical pattern of O3 aCCFs. The resulting aviation NOx emissions are then released in an atmospheric chemistry–climate simulation to simulate the contribution of these NOx emissions to atmospheric O3 and the resulting O3 change. Within this study, we use O3-RF as a proxy for climate impact. The results confirm that the climate-optimised flights lead to lower O3-RF compared to the cost-optimised flights, although the aCCFs cannot reproduce all aspects of the significant impact of the synoptic situation on the transport of emitted NOx. Overall, the climate impact is higher for the selected summer day than for the selected winter day. Lateral re-routing shows a greater potential to reduce climate impact compared to vertical re-routing for the chosen flight altitude. We find that while applying the O3 aCCFs in trajectory optimisation can reduce the climate impact, there are certain discrepancies in the prediction of O3 impact from aviation NOx emissions, as seen for the summer day. Although the O3 aCCFs concept is a rough simplification in estimating the climate impact of a local NOx emission, it enables a reasonable first estimate. Further research is required to better describe the O3 aCCFs allowing an improved estimate in the Average Temperature Response (ATR) of O3 from aviation NOx emissions. A general improvement in the scientific understanding of non-CO2 aviation effects could make climate-optimised flight planning practically feasible.
Biofortification of Soybean (Glycine max L.) through FeSO4·7H2O to Enhance Yield, Iron Nutrition and Economic Outcomes in Sandy Loam Soils of India
Authors: Dhaliwal Salwinder Singh , Sharma Vivek , Shukla Arvind Kumar , Kaur Janpriya , Verma Vibha , Kaur Manmeet , Singh Prabhjot , Kaur Lovedeep , Verma Gayatri , Singh Jagdish , Gaber Ahmed , Hossain Akbar
Published: April 2022

The nutritional value of Glycine max L. (soybean) and its yield potential for improving sustainability of agricultural systems has resulted into its increased production. Soybean crop has potential to replace the rice crop in the rice-wheat cropping system. However, the crop has shown high sensitivity towards iron (Fe) deficiency, and thus recorded major yield and nutritional quality losses. Thus, a three-year field experiment was planned to compare the impact of the application rate (0.5% and 1.0%) and number of sprays of FeSO4 on yield, Fe nutrition, and economic outcomes of soybeans. The Fe application posed a beneficial impact on the studied parameters due to an increase in enzymatic activity of Fe-containing enzymes. Among various treatments, maximum increase in grain and straw yield (3064 and 9341 kg ha−1, respectively) was obtained with 0.5% FeSO4 application at 30, 60, and 90 DAS over the control (2397 and 6894 kg ha−1, respectively). Similar results were attained for grain Fe concentration (69.9 mg kg−1) and Fe uptake in grain and straw (214 and 9088 g ha−1, respectively). The results were statistically non-significant, with the treatment in which 0.5% FeSO4 was applied at 30 and 60 DAS. The economic returns of soybean cultivation were also highest with 0.5% FeSO4 application at 30, 60, and 90 DAS with highest benefit; the cost (3.02) followed by treatment in which 0.5% FeSO4 was applied at 30 and 60 DAS. Thus, 0.5% FeSO4 application at 30, 60, and 90 DAS can be recommended for soybeans grown on sandy loam soil followed by 0.5% FeSO4 application at 30and 60 DAS to harness maximum yield, Fe concentration, and profitability.
Effects of TaMTL-Edited Mutations on Grain Phenotype and Storage Component Composition in Wheat
Authors: Tang Huali , Zhang Shuangxi , Yu Mei , Wang Ke , Yu Yang , Qiu Yuliang , Chang Yanan , Lin Zhishan , Du Lipu , Fu Daolin , Li Zichao , Ye Xingguo
Published: April 2022

Wheat nutrition and processing-quality are primarily based on the endosperm ingredients. However, the effect of embryos on grain traits and components remains unclear. In this study, we found that in the cross-pollinated and self-pollinated progenies of the four wheat mtl mutants (mtl-A, mtl-AD, mtl-BD, and mtl-ABD) the haploid induction rates were 0–15.6% and 0–14.1%, and the embryo abortion rates were 0–27.4% and 0–24.1%, respectively, in which mtl-A had no effect on haploid induction and embryo development. The embryoless grains (ELG) were comparable to the normal grains (NG) from mtl-AD, mtl-BD, and mtl-ABD in grain length, grain width and thousand-kernel weight, but the grain traits were significantly less than those in NG from mutant mtl-A. During grain filling period, mtl-ABD had similar ELG ratio and amount of starch granule (SG) and protein body (PB) in ELG and NG. At maturity stage, the morphological features of A-type and B-type SG in ELG were similar to those in NG in mtl mutants; however, amylose, gliadin, and glutenin contents were higher in ELG, and total starch, albumin and globulin contents were higher in NG. Our results clarified the effect of the wheat mtl mutants on haploid induction and grain traits and nutrition composition in this crop, and provided new clues for studying the development of embryo and endosperm and their interaction in plants.
Combination of Biochar and Phosphorus Solubilizing Bacteria to Improve the Stable Form of Toxic Metal Minerals and Microbial Abundance in Lead/Cadmium-Contaminated Soil
Authors: Lai Weiwei , Wu Yuanyi , Zhang Chaonan , Dilinuer Yimamu , Pasang Lhamo , Lu Yuqi , Wang Yuhong , Chen Haoming , Li Zhen
Published: April 2022

Biochar adsorption and microbial remediation have great potential in the field of soil remediation, but since both are stressed by high concentrations of toxic heavy metals when applied alone, combining the two may become an effective remediation method. In this study, the application effect of phosphorus-solubilizing bacteria (PSB) combined with rice husk biochar on the remediation of Pb/Cd-mixed pollution and the form differences of toxic metal were studied qualitatively and quantitatively. Compared with the contaminated soil, the combined remediation of biochar and PSB significantly increased the pH, carbon and phosphorus by 9.0%, 299.4% and 157.0%. Meanwhile, combined remediation increased the total microbial, bacterial and fungal biomass by 92.11%, 103.13% and 138.10%. This confirmed that the addition of biochar increased the soil nutrients and provided good conditions for PSB or native microorganisms to flourish. The extraction results showed that the stable form of Pb/Cd with biochar + PSB was better than that with biochar/PSB alone. Combined remediation significantly increased the acid-soluble and non-bioavailable fraction of Pb/Cd by 5/15 times and 14/5.8 times in contaminated soil. The acid-soluble and non-bioavailable fractions are the main fraction of toxic metals after combined remediation (>80%). The acid-soluble and non-bioavailable fractions were mainly carbonates and phosphate-based Pb/Cd minerals (XRD analysis). PCA and a GWB model further confirmed that the release of pH and phosphorus was the key to the passivation of Pb/Cd in a short time. Meanwhile, the combination of the biochar (phosphorus supply guarantee) and PSB (acid-soluble phosphorus function) can reduce soil acidification and improve soil nutrients, thus increasing microbial abundance in contaminated soil, even more than that in non-contaminated soil.
Physiological and Transcription Analyses Reveal the Regulatory Mechanism in Oat (Avena sativa) Seedlings with Different Drought Resistance under PEG-Induced Drought Stress
Authors: Gong Wenlong , Ju Zeliang , Chai Jikuan , Zhou Xiangrui , Lin Doudou , Su Weijuan , Zhao Guiqin
Published: April 2022

Drought severely limits the growth and development of oat (Avena sativa) seedlings. As an osmotic regulator simulating a drought environment, Polyethylene glycol (PEG) has been widely linked in response to plant drought tolerance. However, the underlying mechanism of oats’ response to PEG stress is still largely unknown. Here, we investigated the physiological and transcriptome variables of the drought-resistant oat variety DA92-2F6, and the drought-susceptible variety Longyan 3 under 15% PEG-6000 drought stress to better understand the underlying drought tolerance molecular mechanisms. The physiological results showed that except for the cell membrane permeability, the antioxidant enzyme, osmotic adjustment substance, and photosynthetic efficiency were significantly higher in the DA92-2F6 after 7 d stress. Further, 12 cDNA libraries and 123,223 unigenes were obtained by RNA-seq. A total of 33,857 differentially expressed genes (DEGs) were detected, of which two co-upregulated and three co-downregulated in four comparisons. We highlighted an analysis of the DEGs in phytohormone signal transduction pathway. The auxin, cytokinin, and brassinosteroid signaling pathways, were suppressed in Longyan 3, while abscisic acid and jasmonic acid signaling pathways were mainly activated in DA92-2F6 under drought stress. The upregulated of PP2C, ABF, SNRK2, GID1, JAZ, and MYC2 genes may enhance the drought tolerance of DA92-2F6. Taken together, these results provided a new transcript resource for the drought tolerance improvement and a reference for oat drought resistance molecular breeding.
Performance Parameters Optimization of a Three-Row Pneumatic Precision Metering Device for Brassica chinensis
Authors: Sun Xinping , Li Hua , Qi Xindan , Nyambura Samuel Mbugua , Yin Jiaqiao , Ma Yunlong , Wang Jinshuang
Published: April 2022

To improve the problem of low seeding efficiency facing the single-row planting mode traditionally used in China, this study aimed to design a novel three-row pneumatic precision metering device. The working principle and main structure were introduced in the paper. The three main factors affecting the seeding performance comprised of vacuum pressure, angular velocity of the metering tray, and taper angle of the sucking hole. A Box-Behnken experiment design having the qualified index and missed seeding index as the experimental index was used, and the results indicated that optimal performance of the metering device was achieved when the vacuum pressure was 2.16 kPa, the angular velocity of the metering tray was 29.43 rpm, and the taper angle of the sucking hole was 61.51°. The qualified index of the inner, middle, and outer ring was 95.12%, 94.68% and 94.24% respectively, while the missed seeding index of the inner, middle, and outer ring was 3.67%, 3.12% and 2.58% respectively under the optimal combination of parameters. The bench test results were basically consistent with the optimized results. This paper can provide support for the design of a three-row pneumatic precision metering device for Brassica chinensis.
Monitoring of a Broad Set of Pharmaceuticals in Wastewaters by High-Resolution Mass Spectrometry and Evaluation of Heterogenous Catalytic Ozonation for Their Removal in a Pre-Industrial Level Unit
Authors: Nannou Christina , Kaprara Efthimia , Psaltou Savvina , Salapasidou Maria , Palasantza Panagiota-Aikaterini , Diamantopoulos Panagiotis , Lambropoulou Dimitra A. , Mitrakas Manassis , Zouboulis Anastasios
Published: April 2022

The removal of contaminants of emerging concern (CECs) occurring in wastewater effluents, such as pharmaceutically active substances (PhACs) and personal care products, pose a big research challenge since they can be a major source of pollution for water bodies and a danger to public health. The objective of this work was to perform a comprehensive monitoring of a broad set of PhACs (>130) in a wastewater treatment plant (WWTP) close to Thessaloniki (Greece), as well as to evaluate the potential of heterogeneous catalytic ozonation for the removal of CECs from wastewater through a continuous flow system. The high-resolution mass spectrometry analysis revealed the highest average concentrations for irbesartan (1817 ng/L). Antihypertensives along with antibiotics, psychiatrics, and β-blockers were found to aggravate the effluents. Removal efficiency after conventional treatment was >30%. The results from catalytic ozonation unit operation indicate that the introduction of a proper solid material that acts as catalyst can enhance the removal of CECs. A preliminary risk assessment using the risk quotient (RQ) revealed that irbesartan and telmisartan entail high acute risk. The overall results underline the urgent need to incessantly monitor PhACs and expand the toxicological studies to establish the sublethal and chronic effects on aquatic organisms.
Vaccination of Calves with the Mycobacterium bovis BCG Strain Induces Protection against Bovine Tuberculosis in Dairy Herds under a Natural Transmission Setting
Authors: Ábalos Pedro , Valdivieso Nicolás , Pérez de Val Bernat , Vordermeier Martin , Benavides María Belén , Alegría-Morán Raúl , Saadi Karina , Wistuba Mathias , Ortega Camila , Sánchez Nicole , Retamal Patricio
Published: April 2022

Bovine tuberculosis (bTB) is a zoonotic disease caused mainly by Mycobacterium bovis, which is associated with major economic losses for milk and meat producers. The objective of this trial was to assess the efficacy of the BCG Russia strain in a cohort study performed under field conditions, with the vaccination of calves in seven dairy farms from a high prevalence area in central Chile. The trial was performed with 501 animals, subcutaneously vaccinated with 2–8 × 105 colony-forming units of BCG, whilst 441 matched control animals received a saline placebo. Peripheral blood was collected at 6, 12 and 18 months post-vaccination, and infection status was determined using the IFNγ release assay in conjunction with the DIVA (Detecting Infected amongst Vaccinated Animals) antigens ESAT-6, CFP-10 and Rv3615c. The BCG vaccine showed a low but significant level of protection of 22.4% (95% CI 4.0 to 36.4) at the end of the trial. However, diverse levels of protection and a variable duration of immunity were observed between trial herds. This diverse outcome could be influenced by the general health condition of calves and their exposition to non-tuberculous mycobacteria. These results suggest that BCG vaccination of dairy calves in a natural transmission setting confers variable protection to animals against bTB in a high prevalence area.
Productive Performance, Serum Antioxidant Status, Tissue Selenium Deposition, and Gut Health Analysis of Broiler Chickens Supplemented with Selenium and Probiotics—A Pilot Study
Authors: Deng Shengting , Hu Shengjun , Xue Junjing , Yang Kaili , Zhuo Ruiwen , Xiao Yuanyuan , Fang Rejun
Published: April 2022

The effect and interaction of dietary selenium (Se) and probiotics on three yellow chicken growth performance, tissue Se content, antioxidant capacity, and gut health were studied from 0 to 70 days of age. A total of 400 one-day-old broilers were distributed into four groups (I-Se, O-Se, I-Se + pros, and O-Se + pros groups) consisting of a 2 × 2 factorial design. The main factors were the source of Se (I-Se = inorganic Se: 0.2 mg/kg sodium selenite; O-Se = organic Se: 0.2 mg/kg Selenium yeast) and the level of probiotics (0.5% EM or 0% EM, the component of EM mainly includes Lactobacillus and Yeast at the dose of 2 × 108 cfu/kg and 3 × 107 cfu/kg, respectively). Each treatment had 5 duplicates consisting of 20 broilers. The results showed that the I-Se group had a greater (p < 0.05) ratio of feed: weight gain (F/G) of broilers at Starter (0–35 d) than the other treatments. Compared to the I-Se group, the O-Se group increased (p < 0.05) Se concentrations in the liver, pancreas, breast muscles, thigh muscle, and the activity of total antioxidative capacity (T-AOC) in serum, as well as the relative abundance of Barnesiella and Lactobacillus in cecum. Meanwhile, probiotics enhanced (p < 0.05) Se concentrations in the pancreas, thigh muscle, serum, and the activity of T-AOC and glutathione peroxidase (GSH-Px), the duodenum’s ratio of villi height to crypt depth (V/C), the jejunum villus height and V/C, and the ileum’s villus height. Furthermore, the significant interactions (p < 0.05) between Se sources and the level of probiotics were observed in Se concentrations in the pancreas, thigh muscle, serum, crypt depth of duodenum, and villus height of jejunum of birds, and Barnesiella abundance in the cecal. In conclusion, our results demonstrate that the combination of O-Se + pros can improve broiler early growth performance, tissue Se content in the pancreas, thigh muscle, and serum, promote intestinal development, and regulate the composition of intestinal flora, suggesting a better combination. These findings provide an effective method of nutrient combination addition to improving the performance of three yellow chickens.
An Outstanding Role of Adipose Tissue in Canine Stem Cell Therapy
Authors: Prišlin Marina , Vlahović Dunja , Kostešić Petar , Ljolje Ivana , Brnić Dragan , Turk Nenad , Lojkić Ivana , Kunić Valentina , Karadjole Tugomir , Krešić Nina
Published: April 2022

Adipose tissue, previously known as connective tissue with a role in energy storage, is currently changing the course of treatments in veterinary medicine. Recent studies have revealed one particularly impressive function among all the newly discovered functions of adipose tissue. The interactive cells hosted by adipose tissue, the stromal vascular fraction (SVF), and their role in treating numerous diseases have provided a prospective course of research with positive outcomes in regenerative veterinary medicine (RVM). This review describes the main features of adipose tissue, emphasizing an eclectic combination of cells within the SVF and its thus far researched therapeutic possibilities in canine RVM. An afterwards focus is on a highly researched component of the SVF, adipose-derived mesenchymal stem cells (ASCs), which were shown to have an extraordinary impact relying on several proposed mechanisms of action on mitigating pathologies in canines. Furthermore, ASC therapy showed the most significant results in the orthopaedics field and in neurology, dermatology, ophthalmology, gastroenterology, and hepatology, which elevates the possibilities of ASC therapy to a whole new level. Therefore, this review article aims to raise awareness of the importance of research on cellular components, within abundant and easily accessible adipose tissue, in the direction of regenerative therapy in canines, considering the positive outcomes so far. Although the focus is on the positive aspects of cellular therapy in canines, the researchers should not forget the importance of identifying the potential negative aspects within published and upcoming research. Safe and standardized treatment represents a fundamental prerequisite for positively impacting the lives of canine patients.
Wind Farms and Power Lines Have Negative Effects on Territory Occupancy in Eurasian Eagle Owls (Bubo bubo)
Authors: Husby Magne , Pearson Martin
Published: April 2022

Wind power is useful for reducing greenhouse gas emissions, but the construction and operation might have negative effects on biodiversity. The purpose of this study was to investigate any effects of wind farm and power line construction on territory occupancy in the vulnerable Eurasian eagle owl. We investigated 48 eagle owl territories before and after the whole construction period and a short operation period with the use of sound meters. We found that territorial eagle owls within 4–5 km from the wind farm and power line construction disturbance left their territories to a significantly higher extent (41% reduction in the number of territories with eagle owls) compared with the eagle owls in territories further away (23% reduction). The distance from the nest site to the disturbance was significantly shorter for those territories that were abandoned compared with territories where the birds stayed. Possible reasons for this decline might be a higher mortality caused by collisions, desertion and avoidance of wind power areas caused by the noise and disturbance from their construction. In addition, there are possible indirect effects, for example reductions in prey species may force eagle owls to abandon their territories. The construction period lasted much longer than the period with active wind turbines and power lines in this investigation, but we cannot separate the effects of the two because the investigations were only possible in the eagle owl breeding season, and the wind turbines were activated shortly after the construction period. Our results imply that careful investigations are needed to detect the possible occurrence of eagle owls near any type of construction work. Studies of these territories should strongly influence how and when the construction work can be carried out, but more investigations are needed to find details about the influence of distance.
Identification of Candidate Genes for Pigmentation in Camels Using Genotyping-by-Sequencing
Authors: Bitaraf Sani Morteza , Zare Harofte Javad , Banabazi Mohammad Hossein , Faraz Asim , Esmaeilkhanian Saeid , Naderi Ali Shafei , Salim Nader , Teimoori Abbas , Bitaraf Ahmad , Zadehrahmani Mohammad , Burger Pamela Anna , Asadzadeh Nader , Silawi Mohammad , Taghipour Sheshdeh Afsaneh , Mohammad Nazari Behrouz , Faghihi Mohammad Ali , Roudbari Zahra
Published: April 2022

The coat color of dromedary is usually uniform and varies from black to white, although dark- to light-brown colors are the most common phenotypes. This project was designed to gain knowledge on novel color-related variants using genotyping-by-sequencing (GBS). The association between the SNPs and coat color was tested using MLM (mixed linear models) with kinship matrix. Three GWAS models including white color vs. non-white color, black vs. non-black color, and light-brown vs. dark-brown color were performed. There were no distinct genetic clusters detected based on the color phenotypes. However, admixture occurred among all individuals of the four different coat color groups. We identified nine significant SNPs associated with white color after Bonferroni correction, located close to ANKRD26, GNB1, TSPYL4, TEKT5, DEXI, CIITA, TVP23B, CLEC16A, TMPRSS13, FXYD6, MPZL3, ANKRD26, HFM1, CDC7, TGFBR3, and HACE1 genes in neighboring flanking regions. The 13 significant SNPs associated with black color and the candidate genes were: CAPN7, CHRM4, CIITA, CLEC16A, COL4A4, COL6A6, CREB3L1, DEXI, DGKZ, DGKZ, EAF1, HDLBP, INPP5F, MCMBP, MDK, SEC23IP, SNAI1, TBX15, TEKT5, TMEM189, trpS, TSPYL4, TVP23B, and UBE2V1. The SNAI1 gene interacted with MCIR, ASIP and KIT genes. These genes play a key role in the melanin biosynthetic and pigmentation biological process and melanogenesis biological pathway. Further research using a larger sample size and pedigree data will allow confirmation of associated SNPs and the identified candidate genes.
Assessment of Husbandry Practices That Can Reduce the Negative Effects of Exposure to Low Ammonia Concentrations in Broiler Houses
Authors: Barbosa Leonardo V. S. , De Moura Daniella J. , Estellés Fernando , Ramón-Moragues Adrian , Calvet Salvador , Villagrá Arantxa
Published: April 2022

Ammonia is an important pollutant emitted by broiler litter that can accumulate inside farms, impairing animal health and welfare productivity. An experiment was designed to evaluate of precision husbandry practices such as the effect of ventilation, animal density and growth rate as management options to reduce the adverse effects of ammonia exposure on productive parameters in broiler houses. Two identical experimental rooms were used in this study. They were programmed to differ in ammonia concentration from day 32 of the growing period (10 and 20 ppm in Room 1 and Room 2, respectively). Three treatments were tested in each room: slow growth in high stocking density (SHD), fast growth in low density (FLD) and fast growth in high density (FHD). Animal weight, feed intake and feed conversion ratio were determined weekly. In addition, the immune status of animals was assessed by weighing the organs related to immune response as stress indicators. Increasing ventilation was effective to control ammonia concentrations. Exposure to ammonia caused no significant effect on productive parameters. However, lowering stocking density improved response to higher ammonia concentrations by lowering the feed conversion ratio. No other relevant effects of differential exposure to ammonia were found in fast-growing animals, either at high or low stocking density. The use of slow-growing breeds had no effect on production parameters. Despite having a slower growth rate, their feed conversion ratio was not different from that of fast-growing breeds. The productive performance of slow-growing animals was not affected by the differential exposure to ammonia, but the reduced spleen size would suggest an impairment of the immune system.
Exploiting the Anti-Biofilm Effect of the Engineered Phage Endolysin PM-477 to Disrupt In Vitro Single- and Dual-Species Biofilms of Vaginal Pathogens Associated with Bacterial Vaginosis
Authors: Castro Joana , Sousa Lúcia G. V. , França Ângela , Podpera Tisakova Lenka , Corsini Lorenzo , Cerca Nuno
Published: April 2022

Bacterial vaginosis (BV) is the most frequent vaginal infection in women of reproductive age. It is caused by the overgrowth of anaerobic vaginal pathogens, such as Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia, which are vaginal pathogens detected during the early stages of incident BV and have been found to form multi-species biofilms. Treatment of biofilm-associated infections, such as BV, is challenging. In this study, we tested the role of an investigational engineered phage endolysin, PM-477, in the eradication of dual-species biofilms composed of G. vaginalis–F. vaginae or G. vaginalis–P. bivia. Single-species biofilms formed by these species were also analysed as controls. The effect of PM-477 on biomass and culturability of single- and dual-species biofilms was assessed in vitro using a microtiter plate assay, epifluorescence microscopy, confocal laser scanning microscopy, and quantitative PCR. The results showed that PM-477 was particularly effective in the disruption and reduction of culturability of G. vaginalis biofilms. In dual-species biofilms, PM-477 exhibited lower efficiency but was still able to selectively and significantly eliminate G. vaginalis. Since polymicrobial interactions have been shown to strongly affect the activity of various antibiotics, the activity of PM-477 in dual-species biofilms is a potentially promising result that should be further explored, aiming to completely eradicate multi-species biofilms associated with BV.
Development of New Antimycobacterial Sulfonyl Hydrazones and 4-Methyl-1,2,3-thiadiazole-Based Hydrazone Derivatives
Authors: Angelova Violina T. , Pencheva Tania , Vassilev Nikolay , K-Yovkova Elena , Mihaylova Rositsa , Petrov Boris , Valcheva Violeta
Published: April 2022

Fifteen 4-methyl-1,2,3-thiadiazole-based hydrazone derivatives 3a–d and sulfonyl hydrazones 5a–k were synthesized. They were characterized by 1H-NMR, 13C NMR, and HRMS. Mycobacterium tuberculosis strain H37Rv was used to assess their antimycobacterial activity. All compounds demonstrated significant minimum inhibitory concentrations (MIC) from 0.07 to 0.32 µM, comparable to those of isoniazid. The cytotoxicity was evaluated using the standard MTT-dye reduction test against human embryonic kidney cells HEK-293T and mouse fibroblast cell line CCL-1. 4-Hydroxy-3-methoxyphenyl substituted 1,2,3-thiadiazole-based hydrazone derivative 3d demonstrated the highest antimycobacterial activity (MIC = 0.0730 µM) and minimal associated cytotoxicity against two normal cell lines (selectivity index SI = 3516, HEK-293, and SI = 2979, CCL-1). The next in order were sulfonyl hydrazones 5g and 5k with MIC 0.0763 and 0.0716 µM, respectively, which demonstrated comparable minimal cytotoxicity. All compounds were subjected to ADME/Tox computational predictions, which showed that all compounds corresponded to Lipinski’s Ro5, and none were at risk of toxicity. The suitable scores of molecular docking performed on two crystallographic structures of enoyl-ACP reductase (InhA) provide promising insight into possible interaction with the InhA receptor. The 4-methyl-1,2,3-thiadiazole-based hydrazone derivatives and sulfonyl hydrazones proved to be new classes of lead compounds having the potential of novel candidate antituberculosis drugs.
Ozone Treatment Improves the Texture of Strawberry Fruit during Storage
Authors: Piechowiak Tomasz , Migut Dagmara , Józefczyk Radosław , Balawejder Maciej
Published: April 2022

The major aim of this study was to check whether a cyclic ozonation process will affect the preservation of the texture of strawberries stored at room temperature. Strawberry fruit was stored for 3 days at room temperature and ozonated with gaseous ozone at a concentration of 10 and 100 ppm for 30 min, every 12 h of storage. Research showed that the ozonation process inhibited the texture deterioration of the fruit during storage. The positive effect of ozone was directly related to the inhibition of the activity of enzymes involved in the degradation of the fruit cell walls, as well as indirectly from the improved energy metabolism of the fruit. The higher level of energy charge corresponded to the higher resistance of ozonated fruit to abiotic stress, leading to the maintenance of the integrity of cell membranes and, consequently, to maintaining good hardness of the fruit throughout the storage period.
R Packages for Data Quality Assessments and Data Monitoring: A Software Scoping Review with Recommendations for Future Developments
Authors: Mariño Joany , Kasbohm Elisa , Struckmann Stephan , Kapsner Lorenz A. , Schmidt Carsten O.
Published: April 2022

Data quality assessments (DQA) are necessary to ensure valid research results. Despite the growing availability of tools of relevance for DQA in the R language, a systematic comparison of their functionalities is missing. Therefore, we review R packages related to data quality (DQ) and assess their scope against a DQ framework for observational health studies. Based on a systematic search, we screened more than 140 R packages related to DQA in the Comprehensive R Archive Network. From these, we selected packages which target at least three of the four DQ dimensions (integrity, completeness, consistency, accuracy) in a reference framework. We evaluated the resulting 27 packages for general features (e.g., usability, metadata handling, output types, descriptive statistics) and the possible assessment’s breadth. To facilitate comparisons, we applied all packages to a publicly available dataset from a cohort study. We found that the packages’ scope varies considerably regarding functionalities and usability. Only three packages follow a DQ concept, and some offer an extensive rule-based issue analysis. However, the reference framework does not include a few implemented functionalities, and it should be broadened accordingly. Improved use of metadata to empower DQA and user-friendliness enhancement, such as GUIs and reports that grade the severity of DQ issues, stand out as the main directions for future developments.
Hierarchy Establishment from Nonlinear Social Interactions and Metabolic Costs: An Application to Harpegnathos saltator
Authors: Bustamante-Orellana Carlos , Bai Dingyong , Cevallos-Chavez Jordy , Kang Yun , Pyenson Benjamin , Xie Congbo
Published: April 2022

Social hierarchies are ubiquitous in social groups such as human societies and social insect colonies; however, the factors that maintain these hierarchies are less clear. Motivated by the shared reproductive hierarchy of the ant species Harpegnathos saltator, we have developed simple compartmental nonlinear differential equations to explore how key life-history and metabolic rate parameters may impact and determine its colony size and the length of its shared hierarchy. Our modeling approach incorporates nonlinear social interactions and metabolic theory. The results from the proposed model, which were linked with limited data, show that: (1) the proportion of reproductive individuals decreases over colony growth; (2) an increase in mortality rates can diminish colony size but may also increase the proportion of reproductive individuals; and (3) the metabolic rates have a major impact in the colony size and structure of a shared hierarchy.
On the Absorption and Photoluminescence Properties of Pure ZnSe and Co-Doped ZnSe:Eu3+/Yb3+ Crystals
Authors: Abbasgholi-NA Behnaz , Aldaghri Osamah A. , Ibnouf Khalid Hassan , Madkhali Nawal , Cabrera Humberto
Published: April 2022

Co-doped Zinc selenide (ZnSe) is a promising material because of a high photoluminescence efficiency and wide spectral range emission in the visible region. In this work, ZnSe and Eu3+/Yb3+ co-doped ZnSe crystals were grown by the chemical vapour transport method. Photoluminescence and optical measurements revealed the effect of trivalent rare earth Eu3+/Yb3+ ions on the emission of new lines with enhancement intensity. In the photoluminescence spectrum, some sharp and intense lines were observed that allow for the possibility of covering a broad emission range. Moreover, the optical measurement showed a lower bandgap compared to that of pure ZnSe bulk crystal. This material is suitable for developing optoelectronic devices, which can emit light in the visible and near infrared range with an improved emission efficiency and wide tunability.
Continuous Rotor Dynamics of Multi-Disc and Multi-Span Rotor: A Theoretical and Numerical Investigation on the Identification of Bearing Coefficients from Unbalance Responses
Authors: Wang Aiming , Bi Yujie , Cheng Xiaohan , Yang Jie , Meng Guoying , Xia Yun , Feng Yu
Published: April 2022

Identification of bearings’ stiffness and damping coefficients, which strongly affects the dynamic characteristics of rotors, is another inverse problem of Rotor Dynamics. In this paper, aiming at multi-disc and multi-span rotors, two novel algorithms are proposed for identifying each bearing’s coefficients based on the continuous rotor dynamic analysis method. A linear functional relationship between the main complex coefficients and the cross-coupled complex coefficients is obtained, which eliminates the coupling between the coefficients and the rotor unbalance in the forward problem. Then, Algorithm I is proposed. However, it is only suitable for rolling-bearing. To solve the problem, changing the rotating speed slightly is proposed to solve the difficulty that another set of equations cannot be developed because the slope of the proposed linear function is constant when the rotating speed is maintained at a fixed speed. Then, Algorithm II, which can be applied to both rolling-bearing and oil-journal bearing, is provided. Numerical investigations are conducted to study the two methods. It is indicated that there should be a measuring point, called an adjustment point, near each bearing, whose coefficients should be identified, to obtain high identification accuracy. Moreover, the identification accuracy of the two algorithms is strongly related to sensor resolution. When the measuring errors of all the required unbalance responses are zero or the same, the identification errors are almost equal to zero. In conclusion, the proposed algorithms provide a method for monitoring the stiffness and damping coefficients of all bearings in a multi-disc and multi-span rotor under operation conditions to predict rotor dynamic behavior for the safe and steady running of rotating machines.
Biophotonics in Dentistry
Authors: Daghigh Ahmadi Ehsaneh , Hafeji Saudah , Khurshid Zohaib , Imran Eisha , Zafar Muhammad Sohail , Saeinasab Morvarid , Sefat Farshid
Published: April 2022

The aim of this review paper is to concentrate on the use and application of photonics in dentistry. More than one hundred review and research articles were comprehensively analysed in terms of applications of photonics in dentistry, including surgical applications, as well as dental biomaterials, diagnosis and treatments. In biomedical engineering, various fields, such as biology, chemistry, material and physics, come together in to tackle a disease/disorder either as a diagnostic tool or an option for treatment. Engineers believe that biophotonics is the application of photonics in medicine, whereas photonics is simply a technology for creating and connecting packets of light energy, known as photons. This review paper provides a comprehensive discussion of its main elements, such as photoelasticity, interferometry techniques, optical coherence tomography, different types of lasers, carbon nanotubes, graphene and quantum dots.
A Low-Complexity Channel Estimation Based on a Least-Squares Algorithm in OFDM Systems
Authors: Kao Yung-An , Wu Kun-Feng
Published: April 2022

As the channel frequency responses (CFRs) at virtual pilot subcarriers are assumed to be zero, the estimated CFRs will have a leakage effect for discrete Fourier transform (DFT)-based channel estimation in OFDM systems. The CFRs at odd pilot subcarriers and even pilot subcarriers are related if the number of maximum channel delay points is smaller than or equal to half the number of pilots (including virtual pilots). According to this correlation, we propose a low-complexity least-squares (LS) method to estimate the CFRs at virtual even and odd pilot subcarriers, respectively. This will solve the problem of the leakage effect in DFT-based channel estimation. The proposed method does not need to know the statistical properties of the channel or insert extra pilots as with some estimation methods. Furthermore, although this method has less computation than the LS method, both have almost the same channel estimation efficiency in simulation. The channel estimation efficiency of our proposed method is still similar to that of the LS method, even if the number of maximum channel delay points is greater than half the number of pilots. Therefore, the proposed low-complexity method is very suitable for equalizer hardware implementation.
Optimal Configuration of Hybrid Energy System for Rural Electrification of Community Healthcare Facilities
Authors: Gbadamosi Saheed Lekan , Nwulu Nnamdi I.
Published: April 2022

The unavailability of a constant power supply has been a major problem in remote communities in Africa as it impedes the proper operation of healthcare facilities in these locations. This has deprived inhabitants of free access to good healthcare services, thereby resulting in an increase in maternal and child mortality rates in rural communities in Africa. Therefore, in order to address this problem and render a life-saving intervention for rural dwellers and to also improve their healthcare service delivery, this paper focuses on the optimal configuration of a hybrid energy system for the rural electrification of community healthcare facilities. It presents an analysis of an off-grid hybrid energy system comprised of diesel generators, wind turbines and solar PV with a battery storage system to meet the energy demand of healthcare facilities in a remote community in Nigeria. In this study, hybrid energy systems are considered owing to the high reliability and availability of the intensity of solar radiation and wind speeds in Nigeria. An optimization model was developed which seeks to minimize the operational cost of hybrid energy systems. The proposed model was implemented using four case studies and solved using algebraic modeling language. The results obtained from the sensitivity analysis indicate that the configuration that includes solar PV, wind turbines, a battery storage system and a diesel generator provides the optimum power required for a rural healthcare center with a suitable energy cost and emission reduction from the system of diesel generators.
Computational Analysis of Concrete Flow in a Reinforced Bored Pile Using the Porous Medium Approach
Authors: Kränkel Thomas , Weger Daniel , Beckhaus Karsten , Geppert Fabian , Gehlen Christoph , Timothy Jithender J.
Published: April 2022

In this paper, the flow of concrete in a reinforced bored pile is analysed using computational simulations. In order to reduce the computational time, a porous medium that equally mimics the presence of the reinforcement is used. Experimental measurements are used as bounds on the material parameters describing the flow of fresh concrete. The influence of rheological properties of fresh concrete and the thickness of the porous medium that represents the reinforcements is analysed with a classical U-box simulation. Finally, casting of a bored pile is analysed using computational simulation implementing a porous medium representing the reinforcement cage. The concrete flow behavior and especially the filling of the concrete cover zone is analyzed for casting scenarios with different concretes varying in their rheological behavior. Simulations using the porous medium approach is 10x faster than simulations that explicitly model the reinforcements. Simulation results show that a good workability (low viscosity and low yield stress) of the initial batches of concrete must be maintained throughout pouring to avoid the risk of defect formation in the cover zone.
A Climatology of Mesoscale Convective Systems in Northwest Mexico during the North American Monsoon
Authors: Ramos-Pérez Omar , Adams David , Ochoa-Moya Carlos , Quintanar Arturo
Published: April 2022

Mesoscale Convective Systems (MCS) may vary greatly with respect to their morphology, propagation mechanism, intensity, and under which synoptic-scale conditions as a function of topographic complexity. In this study, we develop a long-term climatology of MCS during the North American Monsoon focusing on MCS morphology, lifecycle, and intensity as well as possible propagation mechanisms. We employ an MCS tracking and classification technique based on 23 years (1995 to 2017) of GOES IR satellite data. MCS intensity is also gauged with 7 years (2011 to 2017) of Vaisala GLD360 lightning data and, finally, monthly and interannual variability in synoptic conditions are examined with ERA5 reanalysis data. Our results based on 1594 identified MCS reveal that 98% are morphologically classified as Persistent Elongated Convective Systems. During the 23 summers (June through September) observed, the number of MCS varied considerably, averaging 70 MCS with minimum of 41 and maximum of 94. MCS typically have an average duration of around 8 h ± with a 2 h standard deviation. Propagation speeds, estimated with Hovmöller diagrams in addition to MCS centroid initial and final position, vary slightly depending on the trajectory. A notable result suggests that MCS propagation speeds are more consistent density currents or cold pools and not gravity waves nor steering-level winds. The results of this study could also provide a dataset for examining larger-scale controls on MCS frequency in addition to assesing convective parameterization and convective-resolving models in regions of complex topography.
Can Environmental Regulation Reduce Urban Haze Concentration from the Perspective of China’s Five Urban Agglomerations?
Authors: Li Xinfei , Li Yueming , Xu Chang , Duan Jingyang , Zhao Wenqi , Cheng Baodong , Tian Yuan
Published: April 2022

Based on the perspective of urban agglomerations, this paper explores the impact mechanism of environmental regulation on haze, and tries to find the most suitable environmental regulation intensity for haze control in urban agglomerations. This paper uses the fixed-effect model and panel threshold model to verify the effect of environmental regulations on haze concentration in 206 cities in China. A grouping test is also conducted to verify whether a regional heterogeneity arises due to different regional development levels for five urban agglomerations and non-five urban agglomerations, respectively. The results show that: (1) In the linear model, strengthening environmental regulation can reduce the haze concentration, but this effect is not significant. The effect of environmental regulation on haze control in the five major urban agglomerations is better than that in the non-five major urban agglomerations; (2) In the nonlinear model, the impact of environmental regulation on haze shows a “U” trend in the five major urban agglomerations and an inverted “U” trend in the non-five major urban agglomerations. Although the results are not significant, we can still conclude that the impact of environmental regulation on haze varies depending on the level of regional economic development. Therefore, the environmental regulation should be formulated according to local conditions; (3) In the threshold model, the impact of environmental regulation on the haze concentration in five major urban agglomerations has a threshold effect. In the five major urban agglomerations, although environmental regulation can effectively reduce haze concentration, the governance effect will weaken as the environmental regulation increases. This study plays a positive role in guiding local governments to adjust environmental regulation intensity according to local conditions and helping local environmental improvement.