bluuebunny/crossref_metadata_2025 huggingface dataset:
The human placenta as a model for training and research in mechanical thrombectomy: Clarifications and use of the chorionic plate veins
Authors: Burel Julien , Cornacchini Jonathan , Garnier Matthieu , Patrier Sophie , Guigné Albane , Gerardin Emmanuel , Papagiannaki Chrysanthi , Sourour Nader , Shotar Eimad , Premat Kévin , Laporte Claire , Clarençon Frédéric
Published: September 2022

Indications for mechanical thrombectomy in acute ischemic stroke are increasing, resulting in the continuous development of new devices and techniques. Therefore, there is a need for a realistic testing and training environment that offers the opportunity to practice different procedures and test the latest devices. Some authors have described the use of the human placenta as a model for neurointerventional surgery, with striking similarities to real-life conditions. This model has many advantages, including its relatively low cost and minimal infrastructure requirements, with fewer ethical concerns compared to animal models. So far, some preparation and set-up details were missing, and only arteries from the chorionic plate were used. This article provides the necessary clarifications and a mapping of the chorionic plate veins, so that the use of this model, which is particularly well suited for mechanical thrombectomy, can be as easy and wide as possible. A video explaining how to prepare the model is provided.
COVID-19 and the risk of CNS demyelinating diseases: A systematic review
Authors: Lotan Itay , Nishiyama Shuhei , Manzano Giovanna S. , Lydston Melissa , Levy Michael
Published: September 2022

Background

Viral infections are a proposed possible cause of inflammatory central nervous system (CNS) demyelinating diseases, including multiple sclerosis (MS), neuromyelitis optica spectrum disorder (NMOSD), and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD). During the past 2 years, CNS demyelinating events associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been reported, but causality is unclear.

Objective

To investigate the relationship between CNS demyelinating disease development and exacerbation with antecedent and/or concurrent SARS-CoV-2 infection.

Methods

A systematic literature review of all publications describing either a new diagnosis or relapse of CNS demyelinating diseases (MS, NMOSD, MOGAD) in association with SARS-CoV-2 infection was performed utilizing PRISMA guidelines. Descriptive statistics were used for data analysis, using a case analysis approach.

Results

Sixty-seven articles met the inclusion criteria for the study. Most of the reported cases of NMOSD (n= 13, 72.2% of reported cases) and MOGAD (n= 27, 96.5% of reported cases) were of new disease onset, presenting with typical clinical and radiographic features of these conditions, respectively. In contrast, reported MS cases varied amongst newly diagnosed cases (n= 10, 10.5% of reported cases), relapses (n= 63, 66.4%) and pseudo-relapses (n= 22, 23.2%). The median duration between COVID-19 infection and demyelinating event onset was 11.5 days (range 0–90 days) in NMOSD, 6 days (range−7 to +45 days) in MOGAD, and 13.5 days (range−21 to +180 days) in MS. Most cases received high-dose corticosteroids with a good clinical outcome.

Conclusion

Based upon available literature, the rate of CNS demyelinating events occurring in the setting of preceding or concurrent SARS-CoV-2 infection is relatively low considering the prevalence of SARS-CoV-2 infection. The clinical outcomes of new onset or relapsing MS, NMOSD, or MOGAD associated with antecedent or concurrent infection were mostly favorable. Larger prospective epidemiological studies are needed to better delineate the impact of COVID-19 on CNS demyelinating diseases.
Intravenous thrombolysis with 0.65 mg/kg r-tPA may be optimal for Chinese mild-to-moderate stroke
Authors: Cui Yu , Yao Zhi-Guo , Chen Hui-Sheng
Published: September 2022

Background

Intravenous recombinant tissue plasminogen activator (r-tPA) with 0.9 mg/kg is the standard treatment for acute ischemic stroke, but it remains unclear whether it is optimal for all patients. We aimed to determine the optimal dose of r-tPA for Chinese stroke based on the data from the INTRECIS study.

Methods

From the INTRECIS cohort, patients receiving intravenous r-tPA within 4.5 h of onset were included. According to r-tPA dose, patients were assigned into seven groups (from 0.60 to 0.90 mg/kg). The primary outcomes were the proportion of excellent functional outcomes and symptomatic intracranial hemorrhage.

Results

Overall, 2,666 patients were included: 156 in 0.60 mg/kg group, 117 in 0.65 mg/kg group, 127 in 0.70 mg/kg group, 188 in 0.75 mg/kg group, 154 in 0.80 mg/kg group, 359 in 0.85 mg/kg group, and 1,565 in 0.90 mg/kg group. After adjustment for baseline characteristics, only 0.65 mg/kg group had significantly higher proportion of excellent functional outcome than 0.90 mg/kg group (79.5 vs. 71.4%, odds ratio = 1.833, 95% CI = 1.006–3.341, adjusted p = 0.048). The subgroup analysis showed no evidence of differences in the odds of having a primary outcome between the two groups by age, admission NIHSS, onset to thrombolysis time, and TOAST classification. There was no significant difference in symptomatic intracranial hemorrhage between groups.

Conclusion

Our study presented the first evidence that intravenous thrombolysis with 0.65 mg/kg r-tPA may be optimal for Chinese mild-to-moderate stroke.

Registration

https://www.clinicaltrials.gov, identifier: NCT 02854592.
Predictors of response to acetylcholinesterase inhibitors in dementia: A systematic review
Authors: Pozzi Federico Emanuele , Conti Elisa , Appollonio Ildebrando , Ferrarese Carlo , Tremolizzo Lucio
Published: September 2022

Background

The mainstay of therapy for many neurodegenerative dementias still relies on acetylcholinesterase inhibitors (AChEI); however, there is debate on various aspects of such treatment. A huge body of literature exists on possible predictors of response, but a comprehensive review is lacking. Therefore, our aim is to perform a systematic review of the predictors of response to AChEI in neurodegenerative dementias, providing a categorization and interpretation of the results.

Methods

We conducted a systematic review of the literature up to December 31st, 2021, searching five different databases and registers, including studies on rivastigmine, donepezil, and galantamine, with clearly defined criteria for the diagnosis of dementia and the response to AChEI therapy. Records were identified through the string:predict*AND respon*AND (acetylcholinesterase inhibitors OR donepezil OR rivastigmine OR galantamine). The results were presented narratively.

Results

We identified 1,994 records in five different databases; after exclusion of duplicates, title and abstract screening, and full-text retrieval, 122 studies were finally included.

Discussion

The studies show high heterogeneity in duration, response definition, drug dosage, and diagnostic criteria. Response to AChEI seems associated with correlates of cholinergic deficit (hallucinations, fluctuating cognition, substantia innominate atrophy) and preserved cholinergic neurons (faster alpha on REM sleep EEG, increased anterior frontal and parietal lobe perfusion after donepezil); white matter hyperintensities in the cholinergic pathways have shown inconsistent results. The K-variant of butyrylcholinesterase may correlate with better response in late stages of disease, while the role of polymorphisms in other genes involved in the cholinergic system is controversial. Factors related to drug availability may influence response; in particular, low serum albumin (for donepezil), CYP2D6 variants associated with reduced enzymatic activity and higher drug doses are the most consistent predictors, while AChEI concentration influence on clinical outcomes is debatable. Other predictors of response include faster disease progression, lower serum cholesterol, preserved medial temporal lobes, apathy, absence of concomitant diseases, and absence of antipsychotics. Short-term response may predict subsequent cognitive response, while higher education might correlate with short-term good response (months), and long-term poor response (years). Age, gender, baseline cognitive and functional levels, and APOE relationship with treatment outcome is controversial.
Physical, functional and sensory properties of bitter chocolates with incorporation of high nutritional value flours
Authors: Quispe-Sanchez Luz , Mestanza Marilu , Goñas Malluri , Gill Elizabeth Renee Ambler , Oliva-Cruz Manuel , Chavez Segundo G.
Published: September 2022

Due to the growing demand for healthy food products, the industry is seeking to incorporate inputs with high nutritional potential to traditional products. The objective of this research was to evaluate the effect of incorporating Lepidium meyenii, Chenopodium pallidicaule, Amaranthus caudatus, Sesamum indicum and Salvia hispanica flours on the physical, chemical, rheological, textural and thermal characteristics, and the degree of sensory acceptance of dark chocolate bars (65% cocoa). To this end, chocolate bars were made with the incorporation of five flours in four doses (1, 2, 3 and 4%), obtaining 20 different formulations compared with a control treatment (without flour addition). It was found that as flour incorporation levels increased, viscosity, antioxidants and particle size of the chocolates increased, but hardness and pH decreased. The addition of the flours also affected the acceptability and microstructure of the chocolate bars. The incorporation of up to 4% of the flours studied improved the degree of acceptance of the chocolates. Consequently, the incorporation of grain flours with high nutritional value can enhance the characteristics of dark chocolates, becoming a technological alternative for the chocolate industry.
Clinical implications of immune checkpoint markers and immune infiltrates in patients with thymic neuroendocrine neoplasms
Authors: Liu Man , Hu Wanming , Zhang Yixuan , Zhang Ning , Chen Luohai , Lin Yuan , Wang Yu , Luo Yanji , Guo Yu , Chen Minhu , Chen Jie
Published: September 2022

The potential response of immune checkpoint blockade (ICB) in thymic neuroendocrine neoplasms (T-NEN) is largely unknown and full of great expectations. The expression of immune checkpoint molecules and immune infiltrates greatly determine the response to ICB. However, studies regarding the immune landscape in T-NEN are scarce. This work was aimed to characterize the immune landscape and its association with clinical characteristics in T-NEN. The expression of programmed cell death protein 1 (PD-1) and its ligand, programmed death ligand-1 (PD-L1), and the density of tumor-infiltrating lymphocytes (TILs), monocytes, and granulocytes were determined by immunohistochemical (IHC) staining on tumor tissues from T-NEN. Immune landscapes were delineated and correlated with clinicopathological factors. We found that T-NEN with increased immune cell infiltration and enhanced expression of PD-1/PD-L1 tended to have restricted tumor size and less metastases. A higher density of CD8+ TILs was associated with a significantly lower rate of bone metastasis. In addition, we presented three cases of T-NEN who progressed after multiple lines of therapies and received ICB for alternative treatment. ICB elicited durable partial responses with satisfactory safety in two patients with atypical carcinoid, but showed resistance in 1 patient with large cell neuroendocrine carcinoma. This innovative study delineated for the first time the heterogeneous immune landscape in T-NEN and identified CD8+ TILs as a potential marker to predict bone metastasis. An “immune-inflamed” landscape with the presence of TILs predominated in T-NEN, making T-NEN a potentially favorable target for ICB treatment. Further judicious designs of “tailor-made” clinical trials of ICB in T-NEN are urgently needed.
Comprehensive analysis of m6A-modified circRNAs in peritoneal metastasis of high grade serious carcinoma of ovary
Authors: Guo Lin , Xu Nini , Qiu Daner , Yang Xiaozhe , Zhao Shasha , Zhao Hongxi
Published: September 2022

Purpose

High-grade serous ovarian cancer (HGSOC) remains the most lethal female cancer due to metastasis. CircRNAs are recently identified to be modified by N6-methyladenosine (m6A) in many cells. However, the significance of m6A-modified circular RNAs (circRNAs) has not been elucidated in HGSOC peritoneal metastasis. Here, we aimed to investigate the participation and potential functions of m6A-modified circRNAs in HGSCO peritoneal metastasis.

Methods

Cancerous tissues were collected from the in situ and the peritoneal metastasis lesions of HGSCO patients. M6A-tagged circRNAs were identified by m6A-modified RNA immunoprecipitation sequencing (m6A-RIP-seq). Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the potential functions of the m6A-modified circRNAs.

Results

For the m6A-modified circRNAs, 259 were upregulated and 227 were downregulated in the peritoneal metastasis than in the situ lesions of HGSCO patients. For the m6A peaks, 1541 were upregulated and 1293 were downregulated in the peritoneal metastasis than in the in situ lesions of HGSCO patients. For the differential expressed circRNAs, 1911(19.6%) were upregulated and 2883(29.6%) were downregulated in the peritoneal metastasis than in the in situ lesions of HGSCO patients. The upregulated m6A-modified circRNAs were associated with the HIF-1 signaling. The downregulated m6A-modified circRNAs were associated with the MAPK signaling.

Conclusions

This work firstly identified the transcriptome-wide map of m6A-modified circRNAs in peritoneal metastasis of HGSCO. Our findings provided novel evidences about the participation of m6A-modified circRNAs via HIF-1 and MAPK signaling and a new insight in molecular target of HGSCO peritoneal metastasis.
Application of tDCS in children with cerebral palsy: A mini review
Authors: Tang Lin , Wu Yuwei , Ma Jiayin , Lu Yan , Wang Ling , Shan Chunlei
Published: September 2022

Cerebral palsy (CP) refers to a group of diseases characterized by persistent central dyskinesia, postural development disorder and activity limitation syndromes caused by nonprogressive brain injury in the developing fetus or infant, which is often accompanied by sensory, cognitive and attention disorders. The routine rehabilitation methods for children with CP mainly include physical therapy, occupational therapy, speech therapy and other methods. In recent years, noninvasive brain stimulation (NIBS), as a relatively new intervention method, has been widely used because of its potential to regulate cortical excitability and plasticity. Transcranial direct current stimulation (tDCS) is an NIBS technique that is easier and more convenient to perform. It does not require patients to remain stationary for a long time or have a significant impact on treatment results due to children's frequent activities. Compared with other NIBS techniques, tDCS has greater flexibility and no strict restrictions on patients' activities; it also helps the therapist conduct occupational therapy or speech therapy while a child receives tDCS, which markedly reduces the treatment time and avoids burnout due to a long treatment duration. Thus, tDCS is a better and more convenient intervention for CP children and warrants further exploration. Accordingly, this article reviews tDCS application in children with CP and discusses tDCS application prospects for such children to promote its expansion in clinical practice.
Effective dose of propofol combined with a low-dose esketamine for gastroscopy in elderly patients: A dose finding study using dixon’s up-and-down method
Authors: Zheng Yuling , Xu Yafei , Huang Bixin , Mai Ying , Zhang Yiwen , Zhang Zhongqi
Published: September 2022

Objective: This study aimed to determine the optimal dose of propofol combined with esketamine to inhibit the response to gastroscope insertion in elderly patients.

Methods: This is a prospective, non-controlled, non-randomized, single-center study. Elderly patients aged 65–80 years were enrolled in the study with the American society of anesthesiologists (ASA) physical status I or II undergoing elective gastroscopy. All patients were administered propofol after an intravenous esketamine at the dosage of 0.3 mg/kg 30 s, the subsequent dose of propofol was determined by the response of the previous patient to gastroscope insertion (choking, body movement, etc.) using Dixon’s up-and-down method. The initial dose of propofol administered to the first elderly patient was 3.0 mg/kg, and the standard ratio of propofol dose in adjacent patients was 0.9. At least six crossover points were obtained before the conclusion of the study. By using Probit analysis the median effective dose (ED50), 95% effective dose (ED95), and the corresponding 95% confidence interval (CI) for propofol were determined.

Results: The study continued until we obtained seven crossover points and 32 elderly patients (17 males and 15 females) were collected. The ED50 of propofol combined with esketamine inhibiting response to gastroscope insertion in elderly patients were found to be 1.479 mg/kg (95% CI 1.331–1.592 mg/kg), and ED95 was found to be 1.738 mg/kg (95% CI 1.614–2.487 mg/kg).

Conclusion: According to the present study, propofol combined with 0.3 mg/kg esketamine is safe and effective for elderly patients undergoing gastroscopy. The ED50 and ED95 doses of propofol inhibiting response to gastroscope insertion in elderly patients when combined with 0.3 mg/kg esketamine were 1.479 and 1.738 mg/kg, respectively, without apparent adverse effects.
Preventing chronic fatigue in Czech young athletes: The features description of the “SmartTraining” mobile application
Authors: Bernaciková Martina , Kumstát Michal , Burešová Iva , Kapounková Kateřina , Struhár Ivan , Sebera Martin , Paludo Ana Carolina
Published: September 2022

This study describes a beta version of a mobile application (app) that focuses on preventing chronic fatigue in Czech youth athletes. The first version of the SmartTraining app was developed for athletes as a way to prevent chronic fatigue via alertness and education. For alertness, a multistage process was developed using a combination of parameters about training responses, such as tiredness, well-being, heart rate, energy balance and psychological, and health-related aspects. According to the combination of the multistage parameter outcomes, the algorithm classifies the risk of fatigue based on semaphore light: green corresponds to low, yellow to moderate and red to high risk. The education presented in the app consisted of written and “animated videos” material about the variables involved in training, such as training demands and athletes’ responses, regeneration, nutrition and communication between athletes, coaches, and parents. Subsequently, a beta version of the app was created and freely available to download for Android or iOS mobile. The app can be used in daily routines to reduce the risk of chronic fatigue from inadequate training dose response. Prevention can minimise the risk of injury or physical and emotional burnout in youth. Informing athletes on how to carefully handle the training factors can improve athletes’ awareness of their performance and health status. Collaboration between sports scientists and the commercial sector allows for the efficient development of an easy-to-use and low-cost tool for use in sports settings. Future steps should be performed to validate the app’s accuracy in its alertness and in the efficiency of the educational process.
Stem canker pathogen Botryosphaeria dothidea inhibits poplar leaf photosynthesis in the early stage of inoculation
Authors: Xing Junchao , Li Min , Li Jinxin , Shen Wanna , Li Ping , Zhao Jiaping , Zhang Yinan
Published: September 2022

Fungal pathogens can induce canker lesions, wilting, and even dieback in many species. Trees can suffer serious physiological effects from stem cankers. In this study, we investigated the effects of Botryosphaeria dothidea (B. dothidea) on Populus bolleana (P. bolleana) leaves photosynthesis and stomatal responses, when stems were inoculated with the pathogen. To provide experimental and theoretical basis for preventing poplar canker early. One-year-old poplar stems were inoculated with B. dothidea using an epidermal scraping method. In the early stage of B. dothidea inoculation (2–14 days post inoculation, dpi), the gas exchange, stomatal dynamics, hormone content, photosynthetic pigments content, chlorophyll fluorescence parameters, and non-structural carbohydrate (NSC) were evaluated to elucidate the pathophysiological mechanism of B. dothidea inhibiting photosynthesis. Compared with the control groups, B. dothidea noteworthily inhibited the net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr), and other photosynthetic parameters of poplar leaves, but stomatal limit value (Ls) increased. Consistent with the above results, B. dothidea also reduced stomatal aperture and stomatal opening rate. In addition, B. dothidea not only remarkably reduced the content of photosynthetic pigments, but also decreased the maximum photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPSII), electron transfer efficiency (ETR), and photochemical quenching coefficient (qP). Furthermore, both chlorophyll and ΦPSII were positively correlated with Pn. In summary, the main reason for the abated Pn under stem canker pathogen was that B. dothidea not merely inhibited the stomatal opening, but hindered the conversion of light energy, electron transfer and light energy utilization of poplar leaves. In general, the lessened CO2 and Pn would reduce the synthesis of photosynthetic products. Whereas, sucrose and starch accumulated in poplar leaves, which may be due to the local damage caused by B. dothidea inoculation in phloem, hindering downward transport of these products.
Why does leader aggressive humor lead to bystander workplace withdrawal behavior?—Based on the dual path perspective of cognition- affection
Authors: Chen Hao , Wang Liang , Bao Jiaying
Published: September 2022

Based on the Cognitive-Affective Personality System Theory, this study takes 443 employees of several Chinese enterprises and their direct superiors as the research objects, then a 1:1 paired survey is carried out at three different time points, and data is processed by Mplus 7.4 software. This study finds from a bystander perspective: leader aggressive humor plays a positive role in bystander affective rumination and bystander workplace anxiety. Both bystander affective rumination and bystander workplace anxiety play a mediation role between leader aggressive humor and bystander workplace withdrawal behavior. Besides, organization-based self-esteem alleviates the positive impact of leader aggressive humor on bystander affective rumination and bystander workplace anxiety, and then moderates the indirect impact of leader aggressive humor on bystander workplace withdrawal behavior through bystander affective rumination and bystander workplace anxiety, respectively. This study has practical guiding significance for promoting the organization to reduce the occurrence of aggressive humor, helping employees better integrate into the organization, and building a harmonious organizational environment.
To be assertive or not to be assertive: That is the question! Students' reactions to sexual harassment in academia
Authors: Cabras Cristina , Sechi Cristina , Agus Mirian , Cois Ester , Casula Clementina , Raffo Luigi , Mosca Oriana
Published: September 2022

Introduction

In the literature, no integrated definition of sexual harassment (SH) occurs but there is clear unanimity about SH being offensive, humiliating, and intimidating behavior. Within academic settings, SH has severe negative effects on students' physical or emotional wellbeing as well as on their ability to succeed academically.

Methods

The aim of this study was to investigate the relationship between sex, gender roles, and the ways to manage SH (assertive and nonassertive reactions) in university students. It was hypothesized that female students would report more nonassertive reactions compared to male students. In addition, following the Bem theory on gender roles and using the self-report tool by the same author, it is hypothesized that female and male students, who are classified as feminine, will report more nonassertive responses, whereas male and female students, who are classified as masculine, will report more assertive responses. Our hypothesis was tested with a sample of 1,415 university students (593 men, 41.9%, and 822 women, 58.1%) who completed a questionnaire approved by the local ethical review board for research from the end of January 2019 to the first half of February 2019.

Results

Contrary to our hypothesis, results showed that women react more than men in both assertive and nonassertive modalities. In addition, our results confirmed the main effect of both sex and gender roles on students' assertive and nonassertive reactions to SH in academia.

Conclusion

Educational programs about SH may prove useful in preventing its occurrence. Gender equality plans in academia can improve a nonsexist and safe environment for students. It is urgent to improve transparency and accountability of policies on the management of SH: academic institutions need to formulate a procedure to facilitate SH reporting, considering the sensitive balance of confidentiality and transparency issues. Support for the victims (social services, healthcare, legal representation, and advice concerning career/professional development) must be included.
Number comparison under the Ebbinghaus illusion
Authors: Liu Wei , Wang Chunhui , Zhao Xiaoke , Deng Shixin , Zhao Yajun , Zhang Zhijun
Published: September 2022

A series of studies show interest in how visual attributes affect the estimate of object numbers in a scene. In comparison tasks, it is suggested that larger patches are perceived as more numerous. However, the inequality of density, which changes inversely with the area when numerosity remains constant, may mediate the influence of area on numerosity perception. This study aims to explore the role of area and density in the judgment of numerosity. The Ebbinghaus illusion paradigm was adopted to induce differences in the perceived, rather than the physical, area of the two patches to be compared. Participants were asked to compare the area, density, and the number of the two patches in three tasks. To this end, no PSE (point of subjective equality) bias was found in number comparison with randomly distributed dots, although a significant difference was revealed in the perceived area of the two patches. No PSE bias was found in the density comparison, either. For a comparison, density and number tasks were also conducted with regularly distributed dots. No PSE bias was found in density comparison. By contrast, significant PSE bias showed up in number comparison, and larger patches appeared to be more numerous than smaller patches. The density mechanism was proposed as the basis for number comparison with regular patterns. The individual Weber fractions for regular patterns were not correlated with those for random patterns in the number task, but they were correlated with those for both patterns in the density task. To summarize, numerosity is directly sensed, and numerosity perception is not affected by area inequality induced by the Ebbinghaus illusion. In contrast, density and area are combined to infer numerosity when the approximate numerosity mechanism is disrupted by dot distribution.
Childhood maltreatment increases the suicidal risk in Chinese schizophrenia patients
Authors: Cheng Peng , Ju Peijun , Xia Qingrong , Chen Yuanyuan , Li Jingwei , Gao Jianliang , Zhang Loufeng , Yan Fanfan , Cheng Xialong , Pei Wenzhi , Chen Long , Zhu Cuizhen , Zhang Xulai
Published: September 2022

Objectives

Childhood trauma might be a modifiable risk factor among adults with serious mental illness. However, the correlation of child trauma and suicide is unclear, which were cited most frequently as the biggest challenge to schizophrenia (SCZ) patients in China. We aim to study relationships between child trauma and suicide in SCZ patients of different disease stages.

Methods

Ninety-one participants were included and divided into two groups, namely, first-episode group (n = 46), relapsed group (n = 45). The Positive and Negative Syndrome Scale was used to evaluate the severity of psychotic symptoms. The Beck's Suicide Intent Scale and The Nurses' Global Assessment of Suicide Risk were conducted by patient self-report to assess suicide symptom. The childhood trauma questionnaire was used to estimate severity of traumatic stress experienced during childhood.

Results

Childhood trauma and different dimensions of suicide were significantly higher in the relapsed group than first-episode group (P < 0.01, respectively). BMI has a significant positive relationship with recent psychosocial stress (β = 0.473, t = 3.521, P < 0.001) in first-episode group. As in relapsed group, BMI has a positive effect between severe mental illness and suicide ideation (β = 0.672, t = 5.949, P < 0.001; β = 0.909, t = 2.463, P < 0.001), Furthermore, emotional neglect presented positively related to the suicide risk and proneness to suicidal behavior (β = 0.618, t = 5.518, P < 0.001; β = 0.809, t = 5.356, P < 0.001).

Conclusion

Relapsed group of patients had significantly more severe childhood trauma, recent psychosocial stress, suicidal risk and proneness to suicidal behavior. BMI and emotional neglect are unique predictors for different dimensions of suicide.
Protective effect of irisin against Alzheimer’s disease
Authors: Chen Kang , Wang Kun , Wang Tianhui
Published: September 2022

Despite being one of the greatest global challenges for health and social care in the 21st century, Alzheimer’s disease (AD) lacks specific medicine. Irisin, an exercise-generated muscle factor, emerges as a potential hormone for AD prevention and treatment because of its role in promoting the browning of white adipose tissue, accelerating energy expenditure, regulating energy metabolism, and improving insulin resistance. The study reviews classic hallmarks of AD and irisin’s physiology before discussing the possible mechanism by which irisin protects against AD in terms of its effects related to molecular biology and cellular biology. Results reveal that irisin sharpens learning memory by inducing the production of brain-derived neurotrophic factor (BDNF), lowers the production of inflammatory factors, protects neurology through astrocytes, and ameliorates AD symptoms by improving insulin resistance. The review aims to facilitate future experimental studies and clinical applications of irisin in preventing and treating AD.
Low SARS-CoV-2 viral load among vaccinated individuals infected with Delta B.1.617.2 and Omicron BA.1.1.529 but not with Omicron BA.1.1 and BA.2 variants
Authors: Selvavinayagam Sivaprakasam T. , Yong Yean Kong , Joseph Narcisse , Hemashree Kannan , Tan Hong Yien , Zhang Ying , Rajeshkumar Manivannan , Kumaresan Anandhazhvar , Kalpana Raghu , Kalaivani Vasudevan , Monika Ayyagari Venkata Devi , Suvaithenamudhan Suvaiyarasan , Kannan Meganathan , Murugesan Amudhan , Narayanasamy Krishnasamy , Palani Sampath , Larsson Marie , Shankar Esaki M. , Raju Sivadoss
Published: September 2022

The rapid spread of SARS-CoV-2 variants in the global population is indicative of the development of selective advantages in emerging virus strains. Here, we performed a case-control investigation of the clinical and demographic characteristics, clinical history, and virological markers to predict disease progression in hospitalized adults for COVID-19 between December 2021 and January 2022 in Chennai, India. COVID-19 diagnosis was made by a commercial TaqPath COVID-19 RT-PCR, and WGS was performed with the Ion Torrent Next Generation Sequencing System. High-quality (<5% of N) complete sequences of 73 Omicron B.1.1.529 variants were randomly selected for phylogenetic analysis. SARS-CoV-2 viral load, number of comorbidities, and severe disease presentation were independently associated with a shorter time-to-death. Strikingly, this was observed among individuals infected with Omicron BA.2 but not among those with the BA.1.1.529, BA.1.1, or the Delta B.1.617.2 variants. Phylogenetic analysis revealed severe cases predominantly clustering under the BA.2 lineage. Sequence analyses showed 30 mutation sites in BA.1.1.529 and 33 in BA.1.1. The mutations unique to BA.2 were T19I, L24S, P25del, P26del, A27S, V213G, T376A, D405N and R408S. Low SARS-CoV-2 viral load among vaccinated individuals infected with Delta B.1.617.2 and the Omicron BA.1.1.529 variant but not with Omicron BA.1.1 or BA.2 suggests that the newer strains are largely immune escape variants. The number of vaccine doses received was independently associated with increased odds of developing asymptomatic disease or recovery. We propose that the novel mutations reported herein could likely bear a significant impact on the clinical characteristics, disease progression, and epidemiological aspects of COVID-19. Surging rates of mutations and the emergence of eclectic variants of SARS-CoV-2 appear to impact disease dynamics.
Human trafficking and the growing malady of disinformation
Authors: Prakash Jaya , Erickson Timothy B. , Stoklosa Hanni
Published: September 2022

Disinformation has endangered the most vulnerable communities within our world. The anti-trafficking movement in particular has been adversely impacted by disinformation tactics advanced through the QAnon campaign. QAnon's extremist messaging exacerbates gendered, racist, and xenophobic manifestations of trafficking victimization as well as problematic responses to trafficking that underpin historic structural inequities built into the United States' response to trafficking. We describe an overview of mechanisms used by the QAnon campaign to spread disinformation and illustrate how these mechanisms adversely affect the anti-trafficking movement. Given the critical role of healthcare providers in both the identification and connection to care for trafficked persons, as well as their susceptibility to disinformation, we provide several recommendations for the health sector to leverage their educational and advocacy power to combat trafficking disinformation while addressing the root causes of human trafficking.
Using Microbes to Produce Hydrogen From Garbage
Authors: Montoya-Rosales José de Jesús , Razo-Flores Elías
Published: September 2022

What if it was mentioned that there are good microbes that can eat garbage? There may be reports stating that microbes are bad and cause diseases. However, there are also good microbes that can eat the organic garbage (like banana peels and old vegetables) generated in houses or restaurants. These microbes can use garbage to produce energy, in the form of hydrogen, which can be used to fuel our cars. The process of turning organic garbage into energy is called dark fermentation. During dark fermentation, other helpful compounds are also generated, which can be used to make foods, medicines, beverages, and other useful things. This article discusses the dark fermentation process and the products obtained along the way.
COVID-19-related impact on mental health and career uncertainty in student-athletes—Data from a cohort of 7,025 athletes in an elite sport high school system in Sweden
Authors: Håkansson Anders , Moesch Karin , Kenttä Göran
Published: September 2022

Objectives

Mental health consequences and behavior change has been described in elite athletes following the vast impact of the COVID-19 pandemic on the world of sports. However, most study samples have been of limited size, and few studies have assessed student-athletes. This study aimed to analyze perceived mental health impact, measured as clinical degree of depression and anxiety, worry about one's sport and about one's career, and behavioral change with respect to video gaming behavior, in high-school athletes in Sweden.

Methods

Data on anxiety and depression as well as on perceived behavioral changes during COVID-19 were collected from students at sports high schools in Sweden (N = 7,025) in February 2021, during the ongoing COVID-19 pandemic.

Results

Sixteen and 14% met criteria of moderate/severe depression and anxiety, respectively. Many respondents reported feeling mentally worse during the pandemic (66%), and were worried about the future of their sport (45%) or about their own future in sports (45%). Increased gaming behavior during COVID-19 was reported by 29%. All mental health variables were significantly more common in women, except increased gaming (more common in men). Being worried about one's career was less common in winter sports, more common in team sports and more common in older student-athletes, and associated with both depression and anxiety in regression analyses.

Discussion

Self-reported mental health impact of COVID-19 is substantial in student-athletes, and even more so in women and in team sports. The lower impact in winter athletes suggests a moderating effect of the seasons in which the COVID-19 outbreak occurred.
The diagnostic value of non-invasive methods for diagnosing bladder outlet obstruction in men with lower urinary tract symptoms: A meta-analysis
Authors: Cheng Yu , Li Taicheng , Wu Xiaoyu , Ling Qin , Rao Ke , Yuan Xiaoyi , Chen Zhong , Du Guanghui , Xu Shengfei
Published: September 2022

Purpose

We conducted the first meta-analysis to determine the diagnostic value of non-invasive methods for diagnosing bladder outlet obstruction (BOO) in men with lower urinary tract symptoms (LUTS).

Methods

We searched a range of databases for relevant publications up to June 2022, including PubMed, Embase, Web of Science, and the Cochrane Library. Retrieved studies were then reviewed for eligibility and data were extracted. The risk of bias (RoB) was assessed using the QUADAS-2 tool. We then performed a formal meta-analysis to evaluate the accuracy of various non-invasive methods for diagnosing BOO in men.

Results

We identified 51 eligible studies including 7,897 patients for meta-analysis. The majority of the studies had a low overall RoB. Detrusor wall thickness (DWT) (pooled sensitivity (SSY): 71%; specificity (SPY): 88%; diagnostic odds ratio (DOR): 17.15; area under curve (AUC) 0.87) and the penile cuff test (PCT) (pooled SSY: 87%; SPY: 78%; DOR: 23.54; AUC: 0.88) showed high accuracy for diagnosing BOO. Furthermore, data suggested that DWT had the highest pooled SPY (0.89), DOR (32.58), and AUC (0.90), when using 2 mm as the cut-off.

Conclusion

Of the non-invasive tests tested, DWT and PCT had the highest levels of diagnostic accuracy for diagnosing BOO in men with LUTS. DWT, with a 2 mm cut-off, had the highest level of accuracy. These two methods represent good options as non-invasive tools for evaluating BOO in males.
Low-host double MDA workflow for uncultured ASFV positive blood and serum sample sequencing
Authors: Zhang Chengjun , Cheng Tangyu , Li Dongfan , Yu Xuexiang , Chen Fangzhou , He Qigai
Published: September 2022

African swine fever (ASF) is a highly lethal and contagious disease caused by African swine fever virus (ASFV). Whole-genome sequencing of ASFV is necessary to study its mutation, recombination, and trace its transmission. Uncultured samples have a considerable amount of background DNA, which causes waste of sequencing throughput, storage space, and computing resources. Sequencing methods attempted for uncultured samples have various drawbacks. In this study, we improved C18 spacer MDA (Multiple Displacement Amplification)-combined host DNA exhaustion strategy to remove background DNA and fit NGS and TGS sequencing. Using this workflow, we successfully sequenced two uncultured ASFV positive samples. The results show that this method can significantly reduce the percentage of background DNA. We also developed software that can perform real-time base call and analyses in set intervals of ASFV TGS sequencing reads on a cloud server.
First report of structural characteristics and polymorphisms of the prion protein gene in raccoon dogs: The possibility of prion disease-resistance
Authors: Jo Woo-Sung , Kim Yong-Chan , Oem Jae-Ku , Jeong Byung-Hoon
Published: September 2022

Prion diseases are fatal degenerative encephalopathies caused by misfolded prion protein (PrPSc) converted from normal prion protein (PrPC). Previous studies have reported that genetic polymorphisms of the prion protein gene (PRNP) play a critical role in susceptibility to prion diseases. In addition, prion disease-resistant animals showed unique structural features of prion protein (PrP) related to species-specific amino acids. However, investigations of genetic polymorphisms of the PRNP gene and structural characteristics of PrP have not been performed in raccoon dogs thus far. We investigated genetic polymorphisms of PRNP in 87 raccoon dogs using amplicon sequencing and analyzed the genotype, allele, haplotype frequencies, and linkage disequilibrium (LD) using Haploview version 4.2. In addition, we performed phylogenetic analysis and multiple sequence alignment (MSA) using MEGA X version 10.1.8 and Clustal X version 2.1, respectively. We estimated the impact of raccoon dog and Canidae family-specific amino acids using PolyPhen-2, PROVEAN, and AMYCO. Furthermore, we analyzed the effect of raccoon dog and Canidae family-specific amino acids using the AlphaFold2 and Swiss-PdbViewer programs. We found 4 novel single nucleotide polymorphisms (SNPs) of the raccoon dog PRNP gene. In addition, the raccoon dog PrP showed 99.61% identity and the closest genetic distance to dog PrP. Among the substitutions of Canidae-specific amino acids with interspecific amino acids, D163N showed increased amyloidogenic propensity, and R181H showed alterations of hydrogen bonds. Furthermore, electrostatic potentials were changed according to the substitutions of D163N and R181H. By comparing PrP between raccoon dogs and raccoons, R168K and K224R were found to be related to changes in hydrogen bonds, and K224R altered the electrostatic potential of raccoon dog PrP. In the present study, we first reported 4 novel synonymous SNPs of the raccoon dog PRNP gene. We also identified that the PrP of raccoon dog has high homology (99.61%) with PrP of dog, which is a prion-resistant animal. In addition, raccoon dog PrP-specific amino acids are related to low amyloid propensity and inherent characteristics of 3D structure of raccoon dog PrP compared to the PrP of prion-susceptible species.
Metaphor and the Material Object in Moscow Conceptualism
Authors: Nicholas Mary A.
Published: September 2022

Discussions of conceptual art both East and West have focused on the notion of “dematerialization” of the artwork and the substitution of “art as idea” for concrete works of art. Yet such an approach oversimplifies the role of materiality in works of conceptual art generally and underestimates the transformative role of the concrete object in early Moscow conceptualism in particular. An examination of the Nest, an influential group of artists active from 1974 to 1979, as well as other analytical conceptualists who highlighted materiality in their unofficial art practice suggests that their use of concrete objects and realized metaphors revolutionized late-Soviet unofficial art, moving it from an outdated modernist model of artistic autonomy to a more dynamic and engaged postmodernism. Their previously underappreciated contribution to the evolution of global conceptualism expands our picture of the movement as a whole and provides needed context for late-Soviet art and the post-Soviet period that followed.
Synthesis and Application of Innovative and Environmentally Friendly Photocatalysts: A Review
Authors: Mancuso Antonietta , Iervolino Giuseppina
Published: September 2022

Modern society faces two major challenges: removing pollutants from water and producing energy from renewable sources. To do this, science proposes innovative, low-cost, and environmentally friendly methods. The heterogeneous photocatalysis process fits perfectly in this scenario. In fact, with photocatalysis, it is possible both to mineralize contaminants that are not easily biodegradable and to produce hydrogen from the water splitting reaction or from the conversion of organic substances present in water. However, the main challenge in the field of heterogeneous photocatalysis is to produce low-cost and efficient photocatalysts active under visible light or sunlight. The objective of this review is to compare the new proposals for the synthesis of innovative photocatalysts that reflect the requirements of green chemistry, applied both in the removal of organic contaminants and in hydrogen production. From this comparison, we want to bring out the strengths and weaknesses of the proposals in the literature, but above all, new ideas to improve the efficiency of heterogeneous photocatalysis guaranteeing the principles of environmental and economic sustainability.
Structural Parameter Optimization of the Helical Blade of the Variable-Pitch, Downhole, Cyclone Separator Based on the Response Surface Method
Authors: Nie Qi , Li Meiqiu , Zhou Sizhu
Published: September 2022

Aiming at the problems of pipeline blockage and equipment wear caused by large sand production in shallow gas hydrate mining on the seabed, based on the solid-state fluidization mining method, the idea of in situ separation of natural gas hydrate is proposed, and the downhole design is based on the physical parameters of hydrate mixed slurry. For the in situ helical separator, the CFD-Fluent commercial software was used to establish an analysis model and optimize the response surface of the model. The effects of the three-stage variable-pitch helix and blades on the performance of the separation device were investigated. The simulation results and response surface optimization were conducted through experiments to verify the accuracy. The results show that the third-stage pitch has the greatest impact on the separation efficiency and pressure drop, while the first-stage pitch has the least impact. The pressure drop and separation efficiency are fully considered. After the response surface optimization, the optimal three-stage pitch is the first-stage pitch x1 = 72.227 mm, the pitch of the second stage x2 = 105 mm, and the pitch of the third stage x3 = 124.817 mm. The separation efficiency of the optimal structure is verified by experiments. Compared with the previously used fixed-pitch downhole cyclone separator, the three-stage variable pitch cyclone, the separator improves the separation efficiency from 88.29% to 97.16% while keeping the pressure drop unchanged.
The Modeling of Bubble Lift-Off Diameter in Vertical Subcooled Boiling Flow
Authors: Du Jingyu , Zhao Chenru , Bo Hanliang , Ren Xin
Published: September 2022

Bubble lift-off diameter is characterized as the size of a bubble rising from a wall, which is vital in the boundary condition of heat transfer model and interfacial area transport equation. In this paper, mechanistic force analysis was conducted to explore a predictive model for bubble lift-off diameter in a vertical channel of subcooled boiling flow. Specifically, the component of growth force normal to the wall and the shear lift force lead to the lift-off of a bubble on the vertical surface. Through force analysis, we found that bubble lift-off diameter is arranged to be related to wall superheat, latent heat, liquid velocity, fluid properties, bulk liquid subcooling, etc. To account for the contribution of the influencing factors, the dimensionless bubble lift-off diameter was correlated with dimensionless parameters, including the Prandtl number, the Reynolds number, the Jacob number, and dimensionless subcooling. The proposed correlation was assessed according to experimental data and the predictions showed good agreement with the data.