bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-Concept, Prosocial School Behaviors, Well-Being, and Academic Skills in Elementary School Students: A Whole-Child Perspective
Authors: Wikman Carina , Allodi Mara Westling , Ferrer-Wreder Laura Anne
Published: April 2022

Whole-child development focuses on supporting not only academic but also social emotional skills. This cross-sectional study’s aim was to examine the associations between the child-rated self-concept and well-being, teacher-rated prosocial school behaviors, and academic skills (as measured by child performance tasks) and to examine if there were group differences by gender for these constructs. The sample was 143 s grade students (M age = 8 years old). The results indicate that self-concept correlated highly with well-being and moderately with prosocial behaviors, while academic skills correlated moderately with prosocial behaviors. The results also show that the boys had lower average prosocial behavior (teacher-rated) and self-concept (child-rated) ratings relative to the girls. The findings indicate that prosocial behavior, well-being, and self-concept can be measured in psychometrically sound and practically meaningful ways in Swedish elementary schools. Because many of the socioemotional and behavioral constructs were correlated with academic skills, these constructs could be important targets for early academic support interventions inspired by a whole-child approach.
Adaptively Periodic I/O Scheduling for Concurrent HPC Applications
Authors: Zha Benbo , Shen Hong
Published: April 2022

With the convergence of big data and HPC (high-performance computing), various machine learning applications and traditional large-scale simulations with a stochastically iterative I/O periodicity are running concurrently on HPC platforms, which poses more challenges on the scarcely shared I/O resources due to the ever-growing data transfer demand. Currently the existing heuristic online and periodic offline I/O scheduling methods for traditional HPC applications with a fixed I/O periodicity are not suitable for the applications with stochastically iterative I/O periodicities, which are required to schedule the concurrent I/Os from different applications under I/O congestion. In this work, we propose an adaptively periodic I/O scheduling (APIO) method that optimizes the system efficiency and application dilation by taking the stochastically iterative I/O periodicity of the applications into account. We first build a periodic offline scheduling method within a specified duration to capture the iterative nature. After that, APIO adjusts the bandwidth allocation to resist stochasticity based on the actual length of the computing phrase. In the case where the specified duration does not satisfy the actual running requirements, the period length will be extended to adapt to the actual duration. Theoretical analysis and extensive simulations demonstrate the efficiency of our proposed I/O scheduling method over the existing online approach.
Prompt Frequency Stabilization of Ultra-Stable Laser via Improved Mean Shift Algorithm
Authors: Fan Le , Jiao Dongdong , Liu Jun , Chen Long , Xu Guanjun , Zhang Linbo , Liu Jie , Dong Ruifang , Liu Tao , Zhang Shougang
Published: April 2022

In many scientific fields, the continuous operation of ultra-stable lasers is crucial for applications. To speed up the frequency stabilization process in case of the occurence of unexpected interruptions, a prompt frequency stabilization approach based on an improved mean shift algorithm is proposed and verified with a homemade laser system. We developed a double-loop feedback controller to steer the laser frequency with fast and slow channels, respectively. In this study, an improved mean shift algorithm is utilized to intelligently search for the transmission signal, which involves adaptively updating the sliding window radius and incorporating a Gaussian kernel function to update the shift vector. The number of lock points on the left and right sides of the central point determines the scanning direction to search for the transmission signal quickly. The laser is intentionally interrupted 306 times within 10,000 s to evaluate the relocking performance. The median auto-locking time of the laser is improved from 16 s to 4 s. By beating with another ultra-stable laser system, the laser frequency instability is measured to be less than 2.1×10−14 and the linewidth is 5 Hz. This work improves the adaptation and relocking ability of the ultra-stable laser in a complex environment.
An Overview of Direction-of-Arrival Estimation Methods Using Adaptive Directional Time-Frequency Distributions
Authors: Eranti Pranav Kumar , Barkana Buket D.
Published: April 2022

Direction of arrival (DOA) is one of the essential topics in array signal processing that has many applications in communications, smart antennas, seismology, acoustics, radars, and many more. As the applications of DOA estimation are broadened, the challenges in implementing a DOA algorithm arise. Different environments require different modifications to the existing methods. This paper reviews the DOA algorithms in the literature. It evaluates and compares the performance of the three well known algorithms, including MUSIC, ESPRIT, and Eigenvalue Decomposition (EVD), with and without using adaptive directional time–frequency distributions (ADTFD) at the preprocessing stage. We simulated a case with four sources and three receivers. The sources were well separated. Signals were received at each sensor with an SNR value of −5 dB, 0 dB, 5 dB, and 10 dB. The angles of the sources were 15, 30, 45, and 60 degrees. The simulation results show that the ADTFD algorithm significantly improved the performance of MUSIC, while it did not provide similar results for the ESPRIT and EVD methods. As expected, the computation time of the algorithms was increased by implementing the ADTFD algorithm as a preprocessing step.
Virtual Power Plant Optimization in Smart Grids: A Narrative Review
Authors: Goia Bianca , Cioara Tudor , Anghel Ionut
Published: April 2022

Virtual power plants (VPPs) are promising solutions to address the decarbonization and energy efficiency goals in the smart energy grid. They assume the coordination of local energy resources such as energy generation, storage, and consumption. They are used to tackle problems brought by the stochastic nature of renewable energy, lack of energy storage devices, or insufficient local energy flexibility on the demand side. VPP modeling, management, and optimization are open to research problems that should consider, on one side, the local constraints in the operation of the energy resources and power flows and the energy grid’s sustainability objectives on the other side. There are multiple goals to create a VPP, such as to deliver energy services on a market or to the grid operator, to operate a microgrid in autonomy decoupled from the main grid, or to sustain local energy communities. In this paper, we present the results of a narrative review carried out on the domain of VPP optimization for the local energy grid integration. We have defined a search strategy that considers highly rated international databases (i.e., Elsevier, IEEE, and MDPI) in a six-year timeframe and applied objective inclusion/exclusion criteria for selecting articles and publications for the review; 95 articles have been analyzed and classified according to their objectives and solutions proposed for optimizing VPP integration in smart grids. The results of the study show that VPP concepts and applications are well addressed in the research literature, however, there is still work to be done on: engaging prosumers and citizens in such a virtual organization, developing heuristics to consider a wider range of local and global constraints and non-energy vectors, and to decentralize and make transparent the services delivery and financial settlement towards community members. This study can help researchers to understand the current directions for VPP integration in smart grids. As a next step we plan to further analyze the open research directions related to this problem and target the development of innovative solutions to allow the integration of multi-energy assets and management of cross energy sector services in energy communities.
Corrosion Behavior of Fiber-Reinforced Concrete—A Review
Authors: Naidu Gopu Ganesh , Joseph Sofi Androse
Published: April 2022

Corrosion study of conventional reinforcement in concrete has been accorded wider importance in the last few decades based on the losses occurring in monitoring concrete structures. It is well known that the presence of chloride ions is one of the most significant factors contributing to the corrosion of reinforcing steel. Practically, it is observed that in the marine environment, the activating substances such as chlorides that penetrate the steel can counteract the passivity locally when the electrolyte is highly alkaline. The concrete cover is changed chemically when chloride ionspenetrate into the material, whereupon the pore solution is neutralized. Based on numerous studies, it is evident that steel fibers and glass fibers have less impact on cracked sections in a chloride environment and can oppose chloride infiltration. Glass fibers, when exposed to repeated freeze and thaw conditions, protect the passive layer. This review article highlights the corrosion behavior of reinforced concrete involving various factors such as cracking behavior, transportation, electric conductivity, resistivity, and diffusion of chloride ions in the presence of steel and glass fibers.
Potential Efficiency of Earth Observation for Optimum Fishing Zone Detection of the Pelagic Sardinella aurita Species along the Mediterranean Coast of Egypt
Authors: Ali Elham M. , Zanaty Naglaa , Abou El-Magd Islam
Published: April 2022

This research utilizes the efficiency of remotely sensed data and advanced digital image processing techniques to assist in monitoring for the management of marine fisheries in Egypt (south Mediterranean Sea region). A newly developed model is applied to the Sardinella aurita fisheries to determine optimal fishing zones, and is then applied to monitor and detect the potentially optimal fishing zones for other locally important and economic fish species. This approach depends on chlorophyll-a (Chl-a) and sea-surface temperature (SST) characteristics that are that known to control this species communities. The ranges and distribution of SST and Chl-a were obtained from Moderate Resolution Imaging Spectroradiometer (MODIS) aqua as well as Sentinel-3 satellites during the period from 2018 to 2020. The results revealed that there is a strong link between Sardinella abundance and both the SST and the Chl-a concentration. The suitable months for the abundance of this pelagic fish species were defined to be from May to November, producing 75% of the catch (nearly 42 K tons). Low abundances were recorded during the months from December to April, below the suitable harvest levels for this species. This research highlighted the importance of using satellite data as a tool to help scientists to develop appropriate management strategies for the sustainable management of fisheries resources worldwide.
The Use of γ-Aminobutyric Acid-Producing Saccharomyces cerevisiae SC125 for Functional Fermented Beverage Production from Apple Juice
Authors: Sun Xiangyang , Wang Jie , Li Chanyuan , Zheng Miaoxin , Zhang Qing , Xiang Wenliang , Tang Jie
Published: April 2022

The development of functional fermented beverages enriched with γ-aminobutyric acid (GABA) has been pursued because of the health benefits of GABA; however, few studies have described GABA production by yeast. Therefore, this study aimed to produce fermented apple beverages enriched with GABA produced by Saccharomyces cerevisiae SC125. Golden Delicious apples were fermented by S. cerevisiae SC125 to produce a novel functional beverage; commercial yeast was used as the control. The GABA, organic acid, and volatile compound content during the fermentation process was investigated by high-performance liquid chromatography and headspace solid-phase microextraction/gas chromatography-mass spectrometry. A yield of 898.35 ± 10.10 mg/L GABA was achieved by the efficient bioconversion of L-monosodium glutamate. Notably, the S. cerevisiae SC125-fermented beverage produced several unique volatile compounds, such as esters, alcohols, 6-decenoic acid, and 3-hydroxy−2-butanone, and showed significantly enhanced contents of organic acids, including malic acids, citric acid, and quinic acid. Sensory analysis demonstrated that the S. cerevisiae SC125-fermented apple beverage had improved aroma, flavor, and overall acceptability. In conclusion, a fermented functional apple beverage containing GABA was efficiently produced using S. cerevisiae SC125.
Purchasing Habits, Sustainability Perceptions, and Welfare Concerns of Italian Consumers Regarding Rabbit Meat
Authors: Crovato Stefania , Pinto Anna , Di Martino Guido , Mascarello Giulia , Rizzoli Valentina , Marcolin Silvia , Ravarotto Licia
Published: April 2022

Several factors drive consumer behavior in buying meat, particularly rabbit meat. The consumption of rabbits has decreased in Europe, and the main causes of this trend are an increasing association with the perception of rabbits as pets, consumers’ changes in lifestyle, and eating habits. Additionally, increasing attention is paid to ethical issues regarding animal welfare. Investigating consumers’ knowledge, perceptions, and concerns about rabbit meat production and consumption are crucial for improving market strategies. This study investigated consumers’ perspectives of rabbit meat to provide useful information to producers for promoting this sector to consumers. A mixed-methods research design was applied. Qualitative data were collected through four focus group discussions involving 32 consumers and quantitative data through a semi-structured questionnaire administered nationally and completed by 1001 consumers. The limited use of antibiotics in inbreeding and the absence of animal suffering are factors that most influence consumers’ willingness to purchase rabbit meat. Rural/domestic farms were recognized as places where animal welfare measures can be applied and have a positive influence not only on organoleptic quality but also on ethical value and food safety. The cage was perceived as unsuitable for rabbit growth and was oriented to a process of fattening based on industrial feed and antibiotics. Respondents sought information on rabbit farming during purchase, and the label was the most used tool. The rabbit meat production sector should consider these results to meet consumers’ demands and raise awareness among operators on the use of animal welfare-based farming systems, helping to build a more positive image of the rabbit meat industry.
Effects of Ultrasound Irradiation on the Co-Pigmentation of the Added Caffeic Acid and the Coloration of the Cabernet Sauvignon Wine during Storage
Authors: Xue Zhendan , Wang Tingting , Zhang Qing’an
Published: April 2022

In this paper, experiments were conducted to investigate the effects of ultrasound irradiation on the co-pigmentation of caffeic acid added in wine and the coloration of wine during storage. The wine color, chroma, level of the monomeric, combined and polymerized anthocyanins and the concentrations of malvidin-3-O-glucoside and syringic acid in wines were determined by the high-performance liquid chromatography (HPLC), ultraviolet-visible spectroscopy, respectively. The results indicate that ultrasound irradiation could definitely affect the color characteristic of wine to a certain extent. Compared with the wine without addition of caffeic acid, the co-pigmentation effects of wine added with caffeic acid could be significantly promoted by ultrasound irradiation, such as the wine color, color density and the polymerized anthocyanins. Furthermore, ultrasound irradiation had a continuous effect on the co-pigmentation of caffeic acid and wine coloration with the extended storage time. In summary, ultrasound could significantly modify the color properties of wine by enhancing the co-pigmentation between caffeic acid and monomeric anthocyanins in the wine, resulting in the improvement of wine quality.
Disulfide Bond Engineering for Enhancing the Thermostability of the Maltotetraose-Forming Amylase from Pseudomonas saccharophila STB07
Authors: Wang Yinglan , Li Caiming , Ban Xiaofeng , Gu Zhengbiao , Hong Yan , Cheng Li , Li Zhaofeng
Published: April 2022

Maltooligosaccharides are a novel type of functional oligosaccharides with potential applications in food processing and can be produced by glycosyl hydrolases hydrolyzing starch. However, the main obstacle in industrial applications is the balance between the high temperature of the process and the stability of enzymes. In this study, based on the structural information and in silico tools (DSDBASE-MODIP, Disulfide by Design2 and FoldX), two disulfide bond mutants (A211C-S214C and S409C-Q412C) of maltotetraose-forming amylase from Pseudomonas saccharophila STB07 (MFAps) were generated to improve its thermostability. The mutation A211C-S214C was closer to the catalytic center and showed significantly improved thermostability with a 2.6-fold improved half-life at 60 °C and the thermal transition mid-point increased by 1.6 °C, compared to the wild-type. However, the thermostability of mutant S409C-Q412C, whose mutation sites are closely to CBM20, did not change observably. Molecular dynamics simulations revealed that both disulfide bonds A211C-S214C and S409C-Q412C rigidified the overall structure of MFAps, however, the impact on thermostability depends on the position and distance from the catalytic center.
Ancient Caucasian Wheats: A Contribution for Sustainable Diets and Food Diversity
Authors: Nocente Francesca , Galassi Elena , Taddei Federica , Natale Chiara , Gazza Laura
Published: April 2022

Through the centuries, the domestication and modern breeding of wheat led to a significant loss of genetic variation in the cultivated gene pool with a consequent decrease in food diversity. Current trends towards low-input and sustainable agriculture call for the revitalization and exploitation of ancient wheats, which represent a reservoir of biodiversity useful to ensure sustainable wheat production in the context of climate change and low-input farming systems. Ancient Caucasian wheat species, such as the hulled wheats Triticum timopheevii (tetraploid AuAuGG) and Triticum zhukovskyi (hexaploid AuAuAmAmGG), are still grown to a limited extent in the Caucasus for the production of traditional foods. These Caucasian wheats were grown in Italy and were analyzed for physical, nutritional and technological characteristics and compared to durum wheat. Both Caucasian species revealed a high protein content (on average 18.5%) associated with a low gluten index, mainly in T. zhukovskyi, and test weight values comparable to commercial wheats. The total antioxidant capacity was revealed to be the double of that in durum wheat, suggesting the use of ancient Caucasian wheats for the production of healthy foods. Finally, the technological and rheological results indicated that Caucasian wheats could be potential raw material for the formulation of flat breads, biscuits and pasta.
The Effect of Ozone Treatment on Metabolite Profile of Germinating Barley
Authors: Dong Xue , Sun Litao , Agarwal Manjree , Maker Garth , Han Yitao , Yu Xiangyang , Ren Yonglin
Published: April 2022

Ozone is widely used to control pests in grain and impacts seed germination, a crucial stage in crop establishment which involves metabolic alterations. In this study, dormancy was overcome through after-ripening (AR) in dry barley seed storage of more than 4 weeks; alternatively, a 15-min ozone treatment could break the dormancy of barley immediately after harvest, with accelerated germination efficiency remaining around 96% until 4 weeks. Headspace solid-phase microextraction (HS-SPME) and liquid absorption coupled with gas chromatography mass spectrometry (GC-MS) were utilized for metabolite profiling of 2-, 4- and 7-day germinating seeds. Metabolic changes during barley germination are reflected by time-dependent characteristics. Alcohols, fatty acids, and ketones were major contributors to time-driven changes during germination. In addition, greater fatty acids were released at the early germination stage when subjected to ozone treatment.
Editorial to Special Issue—Research on Isolation and Intelligent Detection Methods of Foodborne Pathogens
Authors: Zhang Daohong , Wang Jianlong
Published: April 2022

Foodborne illnesses pose a significant threat worldwide to public health [...]
Integrated Approach to Achieve a Sustainable Organic Waste Management System in Saudi Arabia
Authors: Abdullah Nibras , Al-wesabi Ola A. , Mohammed Badiea Abdulkarem , Al-Mekhlafi Zeyad Ghaleb , Alazmi Meshari , Alsaffar Mohammad , Anbar Mohammed , Sumari Putra
Published: April 2022

Organic waste management (OWM) has always been a fundamental aspect of human populations. Approaches to OWM must be matched to the characteristics of a certain population. In this consideration, the Kingdom of Saudi Arabia (KSA) is no exception. Organizations are being aligned to focus on sustainability matters sharing significant features with universal trends, especially the integration of 3Rs (reducing waste, reusing, and recycling resources). However, the degree and nature of advancement in the direction of sustainability vary depending on the economic level of a state. High-income economies can afford to pay a higher price to integrate 3Rs technologies. Most recent endeavors have focused on achieving ‘Zero Waste’, which is costly for low-income developing countries. The expectations of OWM systems in KSA must be estimated. In this work, the situations in KSA and other countries are analyzed, and pertinent aspects are explored. Matters relating to the sustainability of OWM are conceptually assessed. This study proposes an integrated method for an organic waste management system to achieve sustainable OWM in the context of state policy and appropriate frameworks, suitable technology, institutional order, operational and monetary administration, and people consciousness and involvement. A genetic-based waste collection transportation algorithm that enhances the efficiency of waste collection truck management is presented in line with this technology. The selected routes based on the Rfs and IPv are the most efficient among those available for the examined smart bin destinations. The minimum Rfs of selected routes is less than the maximum Rfs of available routes by 2.63%. Also, the minimum IPv of selected routes is less than the maximum IPv of available routes by 27.08%. The proposed integrated approach, including the waste collection transportation algorithm, would be beneficial across a variety of country-specific layouts.
Breakthrough Multi-Messenger Astrophysics with the THESEUS Space Mission
Authors: Stratta Giulia , Amati Lorenzo , Branchesi Marica , Ciolfi Riccardo , Tanvir Nial , Bozzo Enrico , Götz Diego , O’Brien Paul , Santangelo Andrea
Published: April 2022

The mission concept THESEUS (Transient High Energy Sky and Early Universe Surveyor) aims at exploiting Gamma-Ray Bursts (GRB) to explore the early Universe, as well as becoming a cornerstone of multi-messenger and time-domain astrophysics. To achieve these goals, a key feature is the capability to survey the soft X-ray transient sky and to detect the faint and soft GRB population so far poorly explored. Among the expected transients there will be high-redshift GRBs, nearby low-luminosity, X-ray Flashes and short GRBs. Our understanding of the physics governing the GRB prompt emission will benefit from the 0.3 keV–10 MeV simultaneous observations for an unprecedented large number of hundreds of events per year. In particular the mission will provide the identification, accurate sky localisation and characterization of electromagnetic counterparts to sources of gravitational wave and neutrino sources, which will be routinely detected during the 2030s by the upgraded second generation and third generation Gravitational Wave (GW) interferometers and next generation neutrino detectors.
One-Step Preparation of Adhesive Composite Hydrogels through Fast and Simultaneous In Situ Formation of Silver Nanoparticles and Crosslinking
Authors: Li Yi , Xiao Yunchao , Xi Man , Li Guibin , Jiang Yang
Published: April 2022

In this study, a series of gelatin/silver nanoparticles (AgNPs) composite hydrogels are prepared for the first time through the facile in situ formation of AgNPs. AgNPs, which are formed by reducing Ag+ using dopamine-conjugated gelatins. These can simultaneously crosslink gelatin molecules, thus generating three-dimentional and porous hydrogels. The gelation time and pore sizes of these composite hydrogels can be controlled by controlling the feeding concentration of AgNO3 and weight content of gelatin in water, respectively. The feeding concentration of AgNO3 also has an effect on the equilibrium swelling ratio of the hydrogels. Moreover, these composite hydrogels, with a controllable gelation time and in situ forming ability, exhibit good adhesive properties and can be used as drug-release depots.
Construction of Macroporous Co2SnO4 with Hollow Skeletons as Anodes for Lithium-Ion Batteries
Authors: Wang Jintian , Wang Junzhang , Guo Xingzhong , Yang Hui
Published: April 2022

Increasing the energy density of lithium-ion batteries (LIBs) can broaden their applications in energy storage but remains a formidable challenge. Herein, with polyacrylic acid (PAA) as phase separation agent, macroporous Co2SnO4 with hollow skeletons was prepared by sol-gel method combined with phase separation. As the anode of LIBs, the macroporous Co2SnO4 demonstrates high capacity retention (115.5% at 200 mA·g−1 after 300 cycles), affording an ultrahigh specific capacity (921.8 mA h·g−1 at 1 A·g−1). The present contribution provides insight into engineering porous tin-based materials for energy storage.
A Co-Polymerizable Linker for the Covalent Attachment of Fibronectin Makes pHEMA Hydrogels Cell-Adhesive
Authors: Schumacher Laura , Siemsen Katharina , Appiah Clement , Rajput Sunil , Heitmann Anne , Selhuber-Unkel Christine , Staubitz Anne
Published: April 2022

Hydrogels are attractive biomaterials because their chemical and mechanical properties can be tailored to mimic those of biological tissues. However, many hydrogels do not allow cell or protein attachment. Therefore, they are post-synthetically functionalized by adding functional groups for protein binding, which then allows cell adhesion in cell culture substrates. However, the degree of functionalization and covalent binding is difficult to analyze in these cases. Moreover, the density of the functional groups and the homogeneity of their distribution is hard to control. This work introduces another strategy for the biofunctionalization of hydrogels: we synthesized a polymerizable linker that serves as a direct junction between the polymeric structure and cell adhesion proteins. This maleimide-containing, polymerizable bio-linker was copolymerized with non-functionalized monomers to produce a bioactive hydrogel based on poly(2-hydroxyethyl methacrylate) (pHEMA). Therefore, the attachment site was only controlled by the polymerization process and was thus uniformly distributed throughout the hydrogel. In this way, the bio-conjugation by a protein-binding thiol-maleimide Michael-type reaction was possible in the entire hydrogel matrix. This approach enabled a straightforward and highly effective biofunctionalization of pHEMA with the adhesion protein fibronectin. The bioactivity of the materials was demonstrated by the successful adhesion of fibroblast cells.
Status and Prospect of Drilling Fluid Loss and Lost Circulation Control Technology in Fractured Formation
Authors: Yang Jingbin , Sun Jinsheng , Bai Yingrui , Lv Kaihe , Zhang Guodong , Li Yuhong
Published: April 2022

Lost circulation in fractured formation is the first major technical problem that restricts improvements in the quality and efficiency of oil and gas drilling engineering. Improving the success rate of one-time lost circulation control is an urgent demand to ensure “safe, efficient and economic” drilling in oilfields all over the world. In view of the current situation, where drilling fluid loss occurs and the plugging mechanism of fractured formation is not perfect, this paper systematically summarizes the drilling fluid loss mechanism and model of fractured formation. The mechanism and the main influencing factors to improve the formation’s pressure-bearing capacity, based on stress cage theory, fracture closure stress theory, fracture extension stress theory and chemical strengthening wellbore theory, are analyzed in detail. The properties and interaction mechanism of various types of lost circulation materials, such as bridging, high water loss, curable, liquid absorption and expansion and flexible gel, are introduced. The characteristics and distribution of drilling fluid loss in fractured formation are also clarified. Furthermore, it is proposed that lost circulation control technology for fractured formation should focus on the development of big data and intelligence, and adaptive and efficient intelligent lost circulation material should be continuously developed, which lays a theoretical foundation for improving the success rate of lost circulation control in fractured formation.
Functional Hydrogels with Chondroitin Sulfate Release Properties Regulate the Angiogenesis Behaviors of Endothelial Cells
Authors: Wang Haonan , Li Qian , Jiang Yongchao , Wang Xiaofeng
Published: April 2022

Functional hydrogels with properties that mimic the structure of extracellular matrix (ECM) and regulate cell behaviors have drawn much attention in biomedical applications. Herein, gelatin-based hydrogels were designed and loaded with chondroitin sulfate (CS) to endow biological regulation on the angiogenesis behaviors of endothelial cells (ECs). Manufactured hydrogels containing various amounts of CS were characterized via methods including mechanical tests, cytocompatibility, hemolysis, and angiogenesis assays. The results showed that the prepared hydrogels exhibited excellent mechanical stability, cytocompatibility, and hemocompatibility. Additionally, the angiogenesis behaviors of ECs were obviously promoted. However, excessive loading of CS would weaken the effect due to a higher proportion of occupation on the cell membrane. In conclusion, this investigation highlights the great potential of these hydrogels in treating ischemic diseases and accelerating tissue regeneration in terms of regulating the angiogenesis process via CS release.
Complete Mitochondrial Genomes of Five Racerunners (Lacertidae: Eremias) and Comparison with Other Lacertids: Insights into the Structure and Evolution of the Control Region
Authors: Tian Lili , Guo Xianguang
Published: April 2022

Comparative studies on mitochondrial genomes (mitogenomes) as well as the structure and evolution of the mitochondrial control region are few in the Lacertidae family. Here, the complete mitogenomes of five individuals of Eremias scripta (2 individuals), Eremias nikolskii, Eremias szczerbaki, and Eremias yarkandensis were determined using next-generation sequencing and were compared with other lacertids available in GenBank. The circular mitogenomes comprised the standard set of 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and a long non-coding control region (CR). The extent of purifying selection was less pronounced for the COIII and ND2 genes in comparison with the rest of the PCGs. The codons encoding Leucine (CUN), Threonine, and Isolecucine were the three most frequently present. The secondary structure of rRNA of Lacertidae (herein, E. scripta KZL15 as an example) comprised four domains and 28 helices for 12S rRNA, with six domains and 50 helices for 16S rRNA. Five types and twenty-one subtypes of CR in Lacertidae were described by following the criteria of the presence and position of tandem repeats (TR), termination-associated sequence 1 (TAS1), termination-associated sequence 2 (TAS2), conserved sequence block 1 (CBS1), conserved sequence block 2 (CSB2), and conserved sequence block 3 (CSB3). The compositions of conserved structural elements in four genera, Acanthodactylus, Darevskia, Eremias, and Takydromus, were further explored in detail. The base composition of TAS2 – TATACATTAT in Lacertidae was updated. In addition, the motif “TAGCGGCTTTTTTG” of tandem repeats in Eremias and the motif ”GCGGCTT” in Takydromus were presented. Nucleotide lengths between CSB2 and CSB3 remained 35 bp in Eremias and Darevskia. The phylogenetic analyses of Lacertidae recovered the higher-level relationships among the three subfamilies and corroborated a hard polytomy in the Lacertinae phylogeny. The phylogenetic position of E. nikolskii challenged the monophyly of the subgenus Pareremias within Eremias. Some mismatches between the types of CR and their phylogeny demonstrated the complicated evolutionary signals of CR such as convergent evolution. These findings will promote research on the structure and evolution of the CR and highlight the need for more mitogenomes in Lacertidae.
Infrageneric Plastid Genomes of Cotoneaster (Rosaceae): Implications for the Plastome Evolution and Origin of C. wilsonii on Ulleung Island
Authors: Yang JiYoung , Kim Seon-Hee , Pak Jae-Hong , Kim Seung-Chul
Published: April 2022

Cotoneaster is a taxonomically and ornamentally important genus in the family Rosaceae; however, phylogenetic relationships among its species are complicated owing to insufficient morphological diagnostic characteristics and hybridization associated with polyploidy and apomixis. In this study, we sequenced the complete plastomes of seven Cotoneaster species (C. dielsianus, C. hebephyllus, C. integerrimus, C. mongolicus, C. multiflorus, C. submultiflorus, and C. tenuipes) and included the available complete plastomes in a phylogenetic analysis to determine the origin of C. wilsonii, which is endemic to Ulleung Island, Korea. Furthermore, based on 15 representative lineages within the genus, we carried out the first comparative analysis of Cotoneaster plastid genomes to gain an insight into their molecular evolution. The plastomes were highly conserved, with sizes ranging from 159,595 bp (C. tenuipes) to 160,016 bp (C. hebephyllus), and had a GC content of 36.6%. The frequency of codon usage showed similar patterns among the 15 Cotoneaster species, and 24 of the 35 protein-coding genes were predicted to undergo RNA editing. Eight of the 76 common protein-coding genes, including ccsA, matK, ndhD, ndhF, ndhK, petA, rbcL, and rpl16, were positively selected, implying their potential roles in adaptation and speciation. Of the 35 protein-coding genes, 24 genes (15 photosynthesis-related, seven self-replications, and three others) were found to harbor RNA editing sites. Furthermore, several mutation hotspots were identified, including trnG-UCC/trnR-UCU/atpA and trnT-UGU/trnL-UAA. Maximum likelihood analysis based on 57 representative plastomes of Cotoneaster and two Heteromeles plastomes as outgroups revealed two major lineages within the genus, which roughly correspond to two subgenera, Chaenopetalum and Cotoneaster. The Ulleung Island endemic, C. wilsonii, shared its most recent common ancestor with two species, C. schantungensis and C. zabelii, suggesting its potential origin from geographically close members of the subgenus Cotoneaster, section Integerrimi.
Advances in RNA-Silencing-Related Resistance against Viruses in Potato
Authors: Jiang Lili , Du Zunhe , Zhang Guizhi , Wang Teng , Jin Guanghui
Published: April 2022

Potato is a major food crop that has the potential to feed the increasing global population. Potato is the fourth most important crop and a staple food for many people worldwide. The traditional breeding of potato poses many challenges because of its autotetraploid nature and its tendency toward inbreeding depression. Moreover, potato crops suffer considerable production losses because of infections caused by plant viruses. In this context, RNA silencing technology has been successfully applied in model and crop species. In this review, we describe the RNA interference (RNAi) mechanisms, including small-interfering RNA, microRNA, and artificial microRNA, which may be used to engineer resistance against potato viruses. We also explore the latest advances in the development of antiviral strategies to enhance resistance against potato virus X, potato virus Y, potato virus A, potato leafroll virus, and potato spindle tuber viroid. Furthermore, the challenges in RNAi that need to be overcome are described in this review. Altogether, this report would be insightful for the researchers attempting to understand the RNAi-mediated resistance against viruses in potato.
A Dispersion Index for the Analysis of the Distribution of Activities in the Tunisian Coastal City of Nabeul
Authors: Bakhtyari Narjiss , Rejeb Bouzgarrou Asma , Claramunt Christophe , Rejeb Hichem
Published: April 2022

This research investigates the duality of the spatial organization and urban activities generated by the coastalization processes of the city of Nabeul. The first part of the study analyzes the city’s fragmentations of the urban landscape using a novel generic index of directional dispersion and a set of space syntax metrics. These structural and functional properties are studied by the concentration and/or dispersion of urban functions of the evolution and development patterns. Among the emerging features, we observe a dispersion of urban activities beyond a central radius all along the western periphery of the city, confirming the phenomenon of urban sprawl that many Tunisian cities are experiencing. These spaces generate urban fragmentations of “new polarity zones” under the influence of the coastline attraction. Finally, this study introduces a novel approach for identifying urban structural polarity and activities, as well as new perspectives for coastal land management and planning.
Italian Translation, Adaptation, and Validation of the Novel Satisfaction Measure Assessment after Primary Total Joint Arthroplasty: The Goodman Score Questionnaire
Authors: Ulivi Michele , Orlandini Luca , Meroni Valentina , Viganò Marco , D’Errico Mario , Perrotta Riccardo , Nannini Alessandra , Peretti Giuseppe M. , Mangiavini Laura
Published: April 2022

Patient satisfaction after total joint arthroplasties (TJA) represents a key element for the evaluation of surgery success in relation to subjects’ needs and expectations. The assessment tools are applied inconsistently throughout the literature, and thus, it is difficult to compare results among different studies. Goodman et al. proposed a standardized questionnaire with strong psychometric properties for the assessment of satisfaction. The present study aims to translate, adapt, and validate the Goodman questionnaire for the Italian population. After translation and back translation, the questionnaire was administrated to 50 patients. Internal consistency, test–retest reliability, floor and ceiling effects, and construct validity were evaluated (correlation with KOOS/HOOS, SF-12 PCS/MCS, EQ-5D). Responsiveness was evaluated with respect to SF-12 PCS improvements. The Italian version of the Goodman score questionnaire demonstrated psychometric properties similar to those of the original version. The translated questionnaire showed good internal consistency (Cronbach’s alpha = 0.836) and test–retest reliability (ICC: 0.507). Moderate/strong correlations were observed between the Italian version of the Goodman score and other scores. The score significantly discriminated patients who improved from those who did not improve in SF-12 PCS after treatment. This study provides an adapted and validated Italian version of the Goodman score questionnaire, with psychometric properties similar to those of its original counterpart.
Health, Economic and Social Development Challenges of the COVID-19 Pandemic: Strategies for Multiple and Interconnected Issues
Authors: Panneer Sigamani , Kantamaneni Komali , Palaniswamy Udhayakumar , Bhat Lekha , Pushparaj Robert Ramesh Babu , Nayar Kesavan Rajasekharan , Soundari Manuel Hilaria , Flower F. X. Lovelina Little , Rice Louis
Published: April 2022

The COVID-19-pandemic-related economic and social crises are leading to huge challenges for all spheres of human life across the globe. Various challenges highlighted by this pandemic include, but are not limited to, the need for global health cooperation and security, better crisis management, coordinated funding in public health emergencies, and access to measures related to prevention, treatment and control. This systematic review explores health, economic and social development issues in a COVID-19 pandemic context and aftermath. Accordingly, a methodology that focuses on identifying relevant literature with a focus on meta-analysis is used. A protocol with inclusion and exclusion criteria was developed, with articles from 15 December 2019 to 15 March 2022 included in the study. This was followed by a review and data analysis. The research results reveal that non-pharmaceutical measures like social distancing, lockdown and quarantine have created long-term impacts on issues such as changes in production and consumption patterns, market crashes resulting in the closure of business operations, and the slowing down of the economy. COVID-19 has exposed huge health inequalities across most countries due to social stratification and unequal distribution of wealth and/or resources. People from lower socio-economic backgrounds lack access to essential healthcare services during this critical time for both COVID-19 and other non-COVID ailments. The review shows that there is minimal literature available with evidence and empirical backup; similarly, data/studies from all countries/regions are not available. We propose that there is a need to conduct empirical research employing a trans-disciplinary approach to develop the most effective and efficient strategies to combat the pandemic and its aftermath. There is a need to explore the social and ecological determinants of this contagious infection and develop strategies for the prevention and control of COVID-19 or similar infections in future.
Predicting Areas with High Concentration of the Long-Term Uninsured and Their Association with Emergency Department Usage by Uninsured Patients in South Carolina
Authors: Truong Khoa , Bedi Julie Summey , Zhang Lingling , Draghi Brooke , Shi Lu
Published: April 2022

Background: To predict areas with a high concentration of long-term uninsured (LTU) and Emergency Department (ED) usage by uninsured patients in South Carolina. Methods: American Community Survey data was used to predict the concentration of LTU at the ZIP Code Tabulation Area (ZCTA) level. In a multivariate regression model, the LTU concentration was then modeled to predict ED visits by uninsured patients. ED data came from the restricted South Carolina Patient Encounter data with patients’ billing zip codes. A simulation was conducted to predict changes in the ED visit numbers and rates by uninsured patients if the LTU concentration was reduced to a lower level. Results: Overall, there was a positive relationship between ED visit rates by the uninsured patients and areas with higher concentrations of LTU. Our simulation model predicted that if the LTU concentration for each ZCTA was reduced to the lowest quintile, the ED visit rates by the uninsured would decrease significantly. The greatest reduction in the number of ED visits by the uninsured over a two-year period was for the following primary diagnoses: abdominal pain (15,751 visits), cellulitis and abscess (11,260 visits) and diseases for the teeth and supporting structures (10,525 visits). Conclusions: The provision of primary healthcare services to the LTU could help cut back inappropriate uses of ED resources and healthcare costs.
Future Scenarios of the Data-Driven Healthcare Economy in South Korea
Authors: Choi Ji-Young , Lee Hee-Jo , Lee Myoung-Jin
Published: April 2022

Although data-based healthcare innovation has been spotlighted in South Korea in recent years, previous studies have made little effort to systematically predict various possible future outcomes in the data-driven healthcare economy. This study investigated possible future such scenarios in South Korea by conducting a general morphological analysis (GMA). Seven key factors were identified that will drive the data-driven healthcare economy: the acceptability of data utilization, the level of data literacy, the status of healthcare data regulation, the healthcare data system, medical costs, the convergence of ICT and biotechnology, and the utilization of data in medical services. The main findings are as follows: Four possible scenarios for the data-driven healthcare economy in South Korea were identified. The first scenario suggested mostly optimistic prospects and close associations between factorial values on the various spectra. The second scenario was similar to the first one, except for medical costs. However, the third scenario contrasted with the first, as it entailed relatively pessimistic factorial values. Finally, most of the elements of the current healthcare status quo were maintained in the fourth scenario. This study makes not only an academic contribution, but also has policy implications based on the four scenarios.
Comparison of One-Stage and Two-Stage Intraoperative Uterine Artery Embolization during Cesarean Delivery for Placenta Accreta: Report of Two Clinical Cases at a Tertiary Referral Medical Center
Authors: Lim Zhu-Wei , Lee Wei-Yang , Huang Yuan-Chun , Wu Wan-Ju , Chen Ming
Published: April 2022

Placenta accreta spectrum (PAS) described the anchoring placental villi attached or penetrating into/through the myometrium. PAS is clinically important because of the unpredictable bleeding amount when manually removing the defective decidualization at the endometrial-myometrial interface. Therefore, a multidisciplinary strategy for cesarean delivery with PAS is crucial. Postoperative embolization after cesarean hysterectomy in a hybrid suite was studied by many scientists. In this study, we demonstrated two cases of intraoperative embolization without hysterectomy in a hybrid operating room for cesarean delivery with placenta accreta. Our results show that intraoperative uterine artery embolization with a hybrid suite is a time-preserving and safe method for cesarean delivery with PAS owing to avoiding the risk of morbidity and mortality during patient transfer.
Searching for Silphium: An Updated Review
Authors: Briggs Lisa , Jakobsson Jens
Published: April 2022

From luxury spice to medical cure-all, silphium was a product coveted throughout the ancient world and occupied an essential place in the export economy of ancient Cyrene. The mysterious extinction of the silphium plant in the 1st century CE leaves us with little evidence as to the exact nature of this important agricultural product. In this paper, an historical background on the kingdom of Cyrene is provided, evidence for the nature of the silphium plant is reviewed, how and why it was consumed and traded is discussed. Possible causes of extinction are considered in the context of plant genetics, biometrics, and soil geochemistry. Next, we demonstrate how modern medical studies conducted on possible living relatives can inform us about claims made by ancient authors as to the medical uses of the silphium plant, including its use as a contraceptive and abortifacient. Finally, methods for recovering silphium are explored. We show how underwater archaeology and the search for ancient shipwrecks off the northern coast of Libya may offer our best chance for the recovery of botanical remains of ancient silphium, and how ancient DNA may be able to establish the genetic makeup of this elusive plant.