bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable Enzymatic Synthesis of a Solketal Ester—Process Optimization and Evaluation of Its Antimicrobial Activity
Authors: Mendoza-Ortiz Patricia A. , Gama Rafaela S. , Gómez Omar C. , Luiz Jaine H. H. , Fernandez-Lafuente Roberto , Cren Erika C. , Mendes Adriano A.
Published: February 2020

The present study aims the enzymatic synthesis of solketal palmitate by esterification between solketal and palmitic acid using heptane as solvent. Lipases from Thermomyces lanuginosus (TLL), Candida rugosa type VII (CRL), and Pseudomonas fluorescens (PFL) were immobilized via interfacial activation on rice husk silica functionalized with triethoxy(octyl)silane (Octyl–SiO2) and used as biocatalysts. A loading of 20–22 mg of lipase/g of support was immobilized independently of the studied enzyme. TLL–Octyl–SiO2 was the most active biocatalyst in oil hydrolysis (656.0 ± 23.9 U/g) and ester synthesis (productivity of 6.8 mmol/min.gbiocat), and it has been chosen for further ester synthesis optimization. The effect of some important parameters such as biocatalyst concentration, reaction temperature and acid:alcohol molar ratio on the reaction has been evaluated using a central composite rotatable design at fixed mechanical stirring (240 rpm) and reaction time (15 min). Subsequently, the effect of reactants concentration and molecular sieve concentration has also been examined. Under optimal conditions (56 °C, acid:alcohol molar ratio of 1:3 with a palmitic acid concentration of 1 M, and 20% wt. of TLL–Octyl–SiO2 per volume of reaction mixture), 83% acid conversion was obtained after 150 min of reaction. The biocatalyst retained 87% of its initial activity after seven successive reaction batches. The product was identified by nuclear magnetic resonance analysis. Antimicrobial activity studies showed that the synthesized ester demonstrated antifungal activity against Candida albicans and Candida parapsilosis, with minimum inhibitory concentration (MIC) between 200 and 400 µg/mL, and bacteriostatic/fungistatic action—minimum microbicial concentration (MMC) > 400 µg/mL.
Tendon Extracellular Matrix Remodeling and Defective Cell Polarization in the Presence of Collagen VI Mutations
Authors: Antoniel Manuela , Traina Francesco , Merlini Luciano , Andrenacci Davide , Tigani Domenico , Santi Spartaco , Cenni Vittoria , Sabatelli Patrizia , Faldini Cesare , Squarzoni Stefano
Published: February 2020

Mutations in collagen VI genes cause two major clinical myopathies, Bethlem myopathy (BM) and Ullrich congenital muscular dystrophy (UCMD), and the rarer myosclerosis myopathy. In addition to congenital muscle weakness, patients affected by collagen VI-related myopathies show axial and proximal joint contractures, and distal joint hypermobility, which suggest the involvement of tendon function. To gain further insight into the role of collagen VI in human tendon structure and function, we performed ultrastructural, biochemical, and RT-PCR analysis on tendon biopsies and on cell cultures derived from two patients affected with BM and UCMD. In vitro studies revealed striking alterations in the collagen VI network, associated with disruption of the collagen VI-NG2 (Collagen VI-neural/glial antigen 2) axis and defects in cell polarization and migration. The organization of extracellular matrix (ECM) components, as regards collagens I and XII, was also affected, along with an increase in the active form of metalloproteinase 2 (MMP2). In agreement with the in vitro alterations, tendon biopsies from collagen VI-related myopathy patients displayed striking changes in collagen fibril morphology and cell death. These data point to a critical role of collagen VI in tendon matrix organization and cell behavior. The remodeling of the tendon matrix may contribute to the muscle dysfunction observed in BM and UCMD patients.
Towards an Integrated System as Point-of-Care Device for the Optical Detection of Sepsis Biomarkers
Authors: Giannetti Ambra , Trono Cosimo , Porro Giampiero , Domenici Claudio , Puntoni Mariarita , Baldini Francesco
Published: February 2020

Severe infection and sepsis are a common, expensive, and frequently fatal conditions in critically ill patients. The sepsis diagnosis is not trivial, since it is an extremely complex chain of events involving inflammatory and anti-inflammatory processes, cellular reactions, and circulatory disorders. For these reasons, delay in diagnosis and initiation of drug treatments have shown to be crucial for this pathology. Moreover, a multitude of biomarkers has been proposed, many more than for other pathologies. In order to select optimal treatments for the highly heterogeneous group of sepsis patients and to reduce costs, novel multiplexed tools that better characterize the patient and his or her specific immune response are highly desired. In order to achieve the fundament of drastically improved multi-analyte detection and to attain low limits of detection in diagnostics, the area of point-of-care testing (POCT) technology is developing quickly, leading to the production of instruments, the reliability of which is continuously increasing. For this purpose, a selection of two biomarkers—C-reactive protein (CRP) and neopterin (NP)—was studied in this paper and a fluorescence-based integrated optical system, suitable for future POCT applications, was implemented that is capable of performing the simultaneous measurement of the two different biomarkers in replicate. A limit of detection of 10 and 2.1 µg L−1 was achieved for CRP and NP spiked in commercially available human serum, respectively. Moreover, measurements on both biomarkers were also performed on serum samples collected from septic patients.
A Taxonomy of Techniques for SLO Failure Prediction in Software Systems
Authors: Grohmann Johannes , Herbst Nikolas , Chalbani Avi , Arian Yair , Peretz Noam , Kounev Samuel
Published: February 2020

Failure prediction is an important aspect of self-aware computing systems. Therefore, a multitude of different approaches has been proposed in the literature over the past few years. In this work, we propose a taxonomy for organizing works focusing on the prediction of Service Level Objective (SLO) failures. Our taxonomy classifies related work along the dimensions of the prediction target (e.g., anomaly detection, performance prediction, or failure prediction), the time horizon (e.g., detection or prediction, online or offline application), and the applied modeling type (e.g., time series forecasting, machine learning, or queueing theory). The classification is derived based on a systematic mapping of relevant papers in the area. Additionally, we give an overview of different techniques in each sub-group and address remaining challenges in order to guide future research.
Priority Treatment Leaves Grassland Restoration Vulnerable to Invasion
Authors: Stuble Katharine L. , Young Truman P.
Published: February 2020

Priority effects can be used to promote target species during restoration. Early planting can provide an advantage over later-arriving species, increasing abundance of these early-arrivers in restored communities. However, we have limited knowledge of the indirect impacts of priority effects in restoration. In particular, we do not understand how priority effects impact non-target species. Of particular conservation concern is how these priority effects influence establishment by non-native species. We use a field-based mesocosm experiment to explore the impacts of priority effects on both target and non-target species in California grasslands. Specifically, we seeded native grasses and forbs, manipulating order of arrival by planting them at the same time, planting forbs one year before grasses, planting grasses one year before forbs, or planting each functional group alone. While our study plots were tilled and weeded for the first year, the regional species pool was heavily invaded. We found that, while early-arrival of native grasses did not promote establishment of non-native species, giving priority to native forbs ultimately left our restoration mesocosms vulnerable to invasion by non-native species. This suggests that, in some cases, establishment of non-native species may be an unintended consequence of using priority treatments as a restoration tool.
Urbanization and Human Population Favor Species Richness of Alien Birds
Authors: Lazarina Maria , Tsianou Mariana A. , Boutsis Georgios , Andrikou-Charitidou Aristi , Karadimou Elpida , Kallimanis Athanasios S.
Published: February 2020

Human activities like urbanization and agriculture affect spatial biodiversity patterns. The presence and activities of humans richly benefit alien species, but native species usually decline in human-impacted areas. Considering that the richness of alien and native species are inter-related, we explored the effect of human population density, human-related land uses (agricultural and urban), and natural land area on avian (alien and native) species richness of Massachusetts for two time periods using Generalized Additive Models. Avian alien species richness increased with native species richness in both time periods. Despite the predominant role of native species richness as a major driver of alien species richness, human activities play an important additional role in shaping species richness patterns of established aliens. Human-related land uses (urban and agricultural) and human population favored alien species richness in both time periods. Counter to expectations, human activities were also positively associated to native avian species richness. Possible explanations of these patterns may include habitat heterogeneity, increased availability of resources, and reduced predation risk.
The Diagnosis of Sulfated Hemoglobin (SulfHb) Secondary to Sulfur Dioxide Poisoning Using Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-ToF MS)—A Novel Approach to an Unusual Clinical Problem
Authors: Docherty Suzanne , Zmuidinaite Raminta , Coulson James , Besser Martin , Iles Ray
Published: February 2020

Sulfhemoglobinemia is a rare entity caused by irreversible sulfation of the heme moiety in haemoglobin to form sulfated haemoglobin (SulfHb) and has been caused by H2S arising from certain metabolites of drugs and bacterial infection. Clinical presentation is similar to that of methemoglobin (MetHb). Furthermore, it is often difficult to distinguish between the diagnosis of SulfHb from MetHb in arterial blood gas analysers due to the broad overlap in the optical density (OD) absorption spectra—that of SulfHb swamping the more distinct OD absorption shift seen with MetHb. The presence of SulfHb was suspected in a 73-year-old lady with low oxygen saturation (SaO2 ~75%), central cyanosis, and normal arterial oxygen partial pressure (pO2 ~12 kPa). Repeated arterial blood gas analysis on different systems returned error messages for MetHb quantification. There was an improvement in oxygen saturation and cyanosis after an exchange transfusion. A full OD spectrophotometry (500–700 nm) of the patient’s whole blood was suggestive of the presence of SulfHb, with a minor peak absorption at 620 nm. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) was undertaken on whole blood samples from the patient pre- and post-transfusion, alongside normal controls. These demonstrated the presence of SulfHb in the patient’s blood, identifying sulfur, sulfur monoxide, and sulfur dioxide bound to the heme moiety. This gave vital identification as to the cause of Hb sulfation, which was distinct from that previously reported. Levels fell after the exchange transfusion and were completely eradicated after the correct source, an Epsom Salts constipation tonic, was identified. MALDI-ToF mass spectrometry is a new, rapid, specific, and sensitive diagnostic test for rare hematological syndromes such as SulfHb. In addition, it can identify the specific compounds bound to heme. Here, we provide useful diagnostic evidence as to the source of SulfHb, which was via SO2 rather than the previously described H2S.
Detection of Occult Metastases in Patients with T1 and T2 Stage Lower Lip Squamous Cell Carcinomas after Positive Lymphoscintigraphy
Authors: Prekazi Loxha Mergime , Stubljar David , Jukic Tomislav , Rusinovci Sinan
Published: February 2020

The aim of this study was to detect lower lip squamous cell carcinomas (SCC) that had metastasized to the lymph nodes and to evaluate if neck dissection was necessary for patients with T1 or T2-stage lip cancer after a sentinel lymph node biopsy (SLNB). The study was conducted as a prospective clinical study to detect occult neck metastases in patients with T1 or T2 stage SCC of the lower lip. Thirty-one patients were eligible and underwent echo-ultrasound, computer tomography, magnetic resonance and lymphoscintigraphy (LSG) as diagnostic procedures. LSG was performed on the same day as the surgical procedure, after intradermal injection of 37 Mbq Tc99m-Sn-colloid/mL at four peritumoral sites. In patients with positive LSG results, the sentinel lymph nodes were extracted surgically. The risk factors for cancer development were sun exposure and smoking. The highest accuracy for detecting lymph node enlargements was achieved with magnetic resonance imaging (MRI; 80.7%). LSG showed excellent sensitivity (100%) and negative predictive value (NPV; 100%). Overall, occult metastases were diagnosed with an SLNB in eight (25.8%) patients. According to the results, with great caution, we suggest that an SLNB is reasonable to initiate only for patients with positive sentinel nodes by positive LSG, to be used as a lower morbidity approach for selected patients with T1 and T2 stage cancers.
Investigation of Entropy in Two-Dimensional Peristaltic Flow with Temperature Dependent Viscosity, Thermal and Electrical Conductivity
Authors: Qasim Muhammad , Ali Zafar , Farooq Umer , Lu Dianchen
Published: February 2020

This study comprehensively explores the generalized form of two-dimensional peristaltic motions of incompressible fluid through temperature-dependent physical properties in a non-symmetric channel. Generation of entropy in the system, carrying Joule heat and Lorentz force is also examined. Viscous dissipation is not ignored, for viewing in-depth, effects of heat transmission and entropy production. The modeling of equations is tracked first in fixed and then in wave frame. The resultant set of coupled non-linear equations are solved numerically by utilizing NDSolve in Mathematica. Comparison between NDSolve and the numerical results obtained through bvp4c MATLAB is made for the validation of our numerical codes. The attained results are found to be in excellent agreement. The impact of control parameters on the velocity profiles, pressure gradient, heat transfer, streamlines and entropy production are studied and discussed graphically. It is witnessed that entropy production and heat transfer are increased significantly subject to the enhancement of Hartman number, Brinkman number and electrical conductivity parameter. Hence, choosing appropriate values of physical parameters, performance and efficiency of flow structure and system can be improved. The results reported provide a virtuous insight into bio energy systems providing a useful standard for experimental and extra progressive computational multiphysics simulations.
Editorial Statement and Research Ideas for Efficiency and Anomalies in Stock Markets
Authors: Wong Wing-Keung
Published: February 2020

The Efficient Market Hypothesis states that it is impossible for an investor to outperform the market because all available information is already built into stock prices. However, some anomalies could persist in stock markets while some other anomalies could appear, disappear and re-appear again without any warning. To explore new theories with applications in this direction, in this editorial, we suggest ideas to authors on what types of papers we will accept for publication in the areas of on Efficiency and Anomalies in Stock Markets. We will discuss some papers published in the special issue of Efficiency and Anomalies in Stock Markets.
Bidirectional Short-Circuit Current Blocker for DC Microgrid Based on Solid-State Circuit Breaker
Authors: Wang Lujun , Feng Boyu , Wang Yu , Wu Tiezhou , Lin Huipin
Published: February 2020

In order to solve the imminent problem in that the traditional protection strategy cannot meet time requirements, together with the fact that the rotational inertia of a DC microgrid is small and short-circuit fault develops rapidly, a bidirectional short-circuit current blocker (BSCCB) based on solid-state circuit breaker for a DC microgrid is proposed. Firstly, the bidirectional current blocking circuit structure is proposed based on the analysis of key components. Then, a top-level differential protection strategy is developed based on the aforementioned proposal. Finally, the performance of the blocking circuit is simulated and verified by experiments. The results show that the proposed method can block short-circuit current within 4 ms, and the response speed of the protection strategy is very fast compared with previous approaches. BSCCB also has reclosing, bidirectional blocking and energy releasing functions. The current blocker proposed in this paper can be reused multiple times and has a promising future in low-voltage DC microgrid application.
Reconfigurable Power Converter for Constant Current Underwater Observatory
Authors: Chen Yanhu , Zang Yujia , Yang Canjun , Duan Zhiyong , Zhang Haoyu , Muhammad Gul
Published: February 2020

A constant current (CC) underwater observatory employing the shunt method to provide constant voltage (CV) power for external loads is favored in occasions where shunt-fault tolerance is required. However, low efficiency of CC to CV conversion with the shunt method limits its application, especially in scenarios of varying loads. In this paper, a highly reliable and stable CC/CV converter with better efficiency is introduced based on the proposed novel active soft bypass (ASB) technology and the proposed novel priority-based power management strategy (PPMS). The ASB technology is a method that employs switches and a special control sequence which greatly depresses the large voltage transient presenting on the input side when trying hard bypass redundant modules, and the PPMS makes the system easy to monitor and ensures the absolute reliability of ASB technology. The theoretical study of this novel reconfigurable CC/CV converter and validation experiments on a prototype are carried out, with results showing great improvement in the performance. In addition, the proposed reconfigurable power converter is applied to a coastal observatory in the East China Sea.
Dual Threshold Adaptive Dynamic Migration Strategy of Virtual Resources Based on BBU Pool
Authors: Wang Cheng , Cao Yushi , Zhang Zhili , Wang Weidong
Published: February 2020

The rapid development of mobile communications and the continuous growth of service needs lead to an increase in the number of base stations (BSs). Through virtualization and cloud technology, virtual Baseband Units (BBUs) are deployed on a virtual machine (VM) to build a BBU pool to achieve hardware resource sharing, which not only saves BS construction costs but also facilitates management and control. However, too high or too low server resource utilization in the pool not only affects the performance of the virtual BBU but also increases the maintenance cost of the physical equipment. In this paper, BBUs are virtualized to construct a virtual BBU pool based on the OpenStack cloud architecture and a dual threshold adaptive dynamic migration strategy is proposed in this scenario. Establish upper and lower threshold of resource utilization of the servers in the pool and the strategy determines whether the dynamic migration is triggered according to the resource utilization of each compute node. If the migration is triggered, the strategy selects the virtual resource to be moved out and the target node to realize the dynamic migration to achieve the purpose of balancing the server load and saving energy consumption. The migration strategy proposed in this paper is simulated on Cloudsim and the experimental results show that the strategy can effectively reduce the number of migrations and migration time on the basis of reducing energy consumption and SLA violations. This paper successfully deployed the strategy on the OpenStack platform, which implements dynamic migration autonomously to save the overall energy consumption of the BBU pool, instead of manual operations.
Numerical Investigations of a Counter-Current Moving Bed Reactor for Thermochemical Energy Storage at High Temperatures
Authors: Preisner Nicole Carina , Bürger Inga , Wokon Michael , Linder Marc
Published: February 2020

High temperature storage is a key factor for compensating the fluctuating energy supply of solar thermal power plants, and thus enables renewable base load power. In thermochemical energy storage, the thermal energy is stored as the reaction enthalpy of a chemically reversible gas-solid reaction. Metal oxides are suitable candidates for thermochemical energy storage for solar thermal power plants, due to their high reaction temperatures and use of oxygen as a gaseous reaction partner. However, it is crucial to extract both sensible and thermochemical energy at these elevated temperatures to boost the overall system efficiency. Therefore, this study focuses on the combined extraction of thermochemical and sensible energy from a metal oxide and its effects on thermal power and energy density during discharging. A counter-current moving bed, based on manganese-iron-oxide, was investigated with a transient, one-dimensional model using the finite element method. A nearly isothermal temperature distribution along the bed height was formed, as long as the gas flow did not exceed a tipping point. A maximal energy density of 933 kJ/kg was achieved, when ( Mn , Fe ) 3 O 4 was oxidized and cooled from 1050 ° C to 300 ° C . However, reaction kinetics can limit the thermal power and energy density. To avoid this drawback, a moving bed reactor based on the investigated manganese-iron oxide should combine direct and indirect heat transfer to overcome kinetic limitations.
A Cloud-Based In-Field Fleet Coordination System for Multiple Operations
Authors: Wu Caicong , Chen Zhibo , Wang Dongxu , Song Bingbing , Liang Yajie , Yang Lili , Bochtis Dionysis D.
Published: February 2020

In large-scale arable farming, multiple sequential operations involving multiple machines must be carried out simultaneously due to restrictions of short time windows. However, the coordination and planning of multiple sequential operations is a nontrivial task for farmers, since each operation may have its own set of operational features, e.g., operating width and turning radius. Taking the two sequential operations—hoeing cultivation and seeding—as an example, the seeder has double the width of the hoeing cultivator, and the seeder must remain idle while waiting for the hoeing cultivator to finish two rows before it can commence its seeding operation. A flow-shop working mode can coordinate multiple machines in multiple operations within a field when different operations have different implement widths. To this end, an auto-steering-based collaborative operating system for fleet management (FMCOS) was developed to realize an in-field flow-shop working mode, which is often adopted by the scaled agricultural machinery cooperatives. This paper proposes the structure and composition of the FMCOS, the method of operating strip segmenting, and a new algorithm for strip state updating between successive field operations under an optimal strategy for waiting time conditioning between sequential operations. A simulation model was developed to verify the state-updating algorithm. Then, the prototype system of FMCOS was combined with auto-steering systems on tractors, and the collaborative operating system for the server was integrated. Three field experiments of one operation, two operations, and three operations were carried out to verify the functionality and performance of FMCOS. The results of the experiment showed that the FMCOS could coordinate in-field fleet operations while improving both the job quality and the efficiency of fleet management by adopting the flow-shop working mode.
Real-time Energy Management Strategy for Oil-Electric-Liquid Hybrid System based on Lowest Instantaneous Energy Consumption Cost
Authors: Yang Yang , Zhong Zhen , Wang Fei , Fu Chunyun , Liao Junzhang
Published: February 2020

For the oil–electric–hydraulic hybrid power system, a logic threshold energy management strategy based on the optimal working curve is proposed, and the optimal working curve in each mode is determined. A genetic algorithm is used to determine the optimal parameters. For driving conditions, a real-time energy management strategy based on the lowest instantaneous energy cost is proposed. For braking conditions and subject to the European Commission for Europe (ECE) regulations, a braking force distribution strategy based on hydraulic pumps/motors and supplemented by motors is proposed. A global optimization energy management strategy is used to evaluate the strategy. Simulation results show that the strategy can achieve the expected control target and save about 32.14% compared with the fuel consumption cost of the original model 100 km 8 L. Under the New European Driving Cycle (NEDC) working conditions, the energy-saving effect of this strategy is close to that of the global optimization energy management strategy and has obvious cost advantages. The system design and control strategy are validated.
Natural Dyes from Mortiño (Vaccinium floribundum) as Sensitizers in Solar Cells
Authors: Taco-Ugsha Miguel A. , Santacruz Cristian P. , Espinoza-Montero Patricio J.
Published: February 2020

Photovoltaic energy presents environmental advantages; however, these advantages are limited by the cost of manufacturing solar cells and in many cases, scarce or dangerous materials are incorporated. Therefore, the use of natural dyes from mortiño (Vaccinium floribundum) as sensitizers in solar cells is proposed. The dyes were extracted by maceration in acidified methanol (HCl, citric acid and trifluoroacetic acid TFA) and were characterized by High-Performance Liquid Chromatography (HPLC), Thin-Layer Chromatography (TLC) and spectrometric methods (UV-Vis, IR and MS-MALDI). The construction and characterization of cells were in standard conditions. The study confirms that pigments in mortiño are flavonoids of the anthocyanidin group as: cyanidin-3-galactoside, and cyanidin-3-arabinoside. The efficiency of solar cells was between 0.18–0.26%; the extraction with TFA in methanol leads to the best performance. Although they have low power conversion efficiency, mortiño dyes could be an alternative to artificial sensitizers for solar cell technologies because they are harmless and abundant substances.
Multi-State Household Appliance Identification Based on Convolutional Neural Networks and Clustering
Authors: Zhang Ying , Yin Bo , Cong Yanping , Du Zehua
Published: February 2020

Non-intrusive load monitoring, a convenient way to discern the energy consumption of a house, has been studied extensively. However, most research works have been carried out based on a hypothetical condition that each electric appliance has only one running state. This leads to low identification accuracy for multi-state electric appliances. To deal with this problem, a method for identifying the type and state of electric appliances based on a power time series is proposed in this paper. First, to identify the type of appliance, a convolutional neural network model was constructed that incorporated residual modules. Then, a k-means clustering algorithm was applied to calculate the number of states of the appliance. Finally, in order to identify the states of the appliances, different k-means clustering models were established for different multi-state electric appliances. Experimental results show effectiveness of the proposed method in identifying both the type and the running state of electric appliances.
Modelling Heat Pumps with Variable EER and COP in EnergyPlus: A Case Study Applied to Ground Source and Heat Recovery Heat Pump Systems
Authors: Priarone Antonella , Silenzi Federico , Fossa Marco
Published: February 2020

Dynamic energy modelling of buildings is a key factor for developing new strategies for energy management and consumption reduction. For this reason, the EnergyPlus software was used to model a near-zero energy building (Smart Energy Buildings, SEB) located in Savona, Italy. In particular, the focus of the present paper concerns the modeling of the ground source water-to-water heat pump (WHP) and the air-to-air heat pump (AHP) installed in the SEB building. To model the WHP in EnergyPlus, the Curve Fit Method was selected. Starting from manufacturer data, this model allows to estimate the COP of the HP for different temperature working conditions. The procedure was extended to the AHP. This unit is a part of the air-handling unit and it is working as a heat recovery system. The results obtained show that the HP performance in EnergyPlus can closely follow manufacturer data if proper input recasting is performed for EnergyPlus simulations. The present paper clarifies a long series of missed information on EnergyPlus reference sources and allows the huge amount of EnergyPlus users to properly and consciously run simulations, especially when unconventional heat pumps are present.
Selection and Validation of Appropriate Reference Genes for Real-Time Quantitative PCR Analysis in Needles of Larix olgensis under Abiotic Stresses
Authors: Li Dandan , Yu Sen , Zeng Minzhen , Liu Xiao , Yang Jia , Li Chenghao
Published: February 2020

Larix olgensis Henry is an important afforestation species in northeastern China because of its fast juvenile growth, high-quality timber, and significant economic and ecological values. The selection of appropriate reference genes is necessary for the normalization of gene expression determination during quantitative real-time polymerase chain reaction (qRT-PCR) experiments. In this study, qRT-PCR was used to study gene expression. Three software packages geNorm, NormFinder, BestKeeper were used, and a comprehensive ranking of candidate reference genes was produced based on their output to evaluate the expression stability of 16 candidate reference genes from L. olgensis under drought, salt, cold, and heat stress. PP2A-1 and GAPDH ranked as the most stable reference genes under drought and cold stress, PP2A-1 and UBQ10 were most stable under salt stress, and TIP41 and ACT2 were most stable under heat stress. The least stable gene was ADP, which ranked the last under all treatments. Expression profile analysis of the antioxidant gene CAT using the two most stable and the single least stable reference genes under each stress further verified that the selected reference genes were suitable for gene expression normalization. This study provides an important foundation for the selection of suitable reference genes for the normalization and quantification of L. olgensis gene expression under abiotic stress conditions.
Influence of Heat Treatment and Tannin Impregnation on Boron Depletion and Wood Durability
Authors: Verly Lopes Dercilio Junior , Barnes H. Michael , dos Santos Bobadilha Gabrielly
Published: February 2020

Heat treatment (HT) of a mixture of tannins and inorganic boron compounds showed effective results against wood decay organisms. Boron compounds play a critical role in the preservation of wood against wood decay organisms. The use of tannins and HT represents a relatively new environmentally friendly approach to the wood preservation industry. The aim of this study was to investigate the effect of tannin impregnation and HT on boron depletion, and termite and fungi resistance. Southern yellow pine (SYP) and yellow-poplar (YP) cube samples were used for this study. A mixture of condensed tannins from the Quebracho tree and disodium octaborate tetrahydrate (DOT) was injected into the specimens using a vacuum/pressure cycle, and the specimens were then heat-treated under N2 atmosphere for four hours at 190 °C to investigate both the tannin’s fixative ability to reduce boron leaching and the performance of the mixture against brown and white-rot fungi and termites. Tannins restricted boron leaching in 46% and 34% for SYP and YP, respectively, and also significantly increased the resistance against white-rot fungi for YP. Tannins and HT showed unpredictably good performance against termites. Tannins may be acting to denature proteins; in that case, fungal enzymes would be inhibited. This study revealed the importance of HT on a mixture of boron and tannins to decrease boron leaching and increase the durability of preservative systems.
Comparison of Tree Biomass Modeling Approaches for Larch (Larix olgensis Henry) Trees in Northeast China
Authors: Dong Lihu , Zhang Yue , Zhang Zhuo , Xie Longfei , Li Fengri
Published: February 2020

Accurate quantification of tree biomass is critical and essential for calculating carbon storage, as well as for studying climate change, forest health, forest productivity, nutrient cycling, etc. Tree biomass is typically estimated using statistical models. Although various biomass models have been developed thus far, most of them lack a detailed investigation of the additivity properties of biomass components and inherent correlations among the components and aboveground biomass. This study compared the nonadditive and additive biomass models for larch (Larix olgensis Henry) trees in Northeast China. For the nonadditive models, the base model (BM) and mixed effects model (MEM) separately fit the aboveground and component biomass, and they ignore the inherent correlation between the aboveground and component biomass of the same tree sample. For the additive models, two aggregated model systems with one (AMS1) and no constraints (AMS2) and two disaggregated model systems without (DMS1) and with an aboveground biomass model (DMS2) were fitted simultaneously by weighted nonlinear seemingly unrelated regression (NSUR) and applied to ensure additivity properties. Following this, the six biomass modeling approaches were compared to improve the prediction accuracy of these models. The results showed that the MEM with random effects had better model fitting and performance than the BM, AMS1, AMS2, DMS1, and DMS2; however, when no subsample was available to calculate random effects, AMS1, AMS2, DMS1, and DMS2 could be recommended. There was no single biomass modeling approach to predict biomass that was best for all aboveground and component biomass except for MEM. The overall ranking of models based on the fit and validation statistics obeyed the following order: MEM > DMS1 > AMS2 > AMS1> DMS2 > BM. This article emphasized more on the methodologies and it was expected that the methods could be applied by other researchers to develop similar systems of the biomass models for other species, and to verify the differences between the aggregated and disaggregated model systems. Overall, all biomass models in this study have the benefit of being able to predict aboveground and component biomass for larch trees and to be used to predict biomass of larch plantations in Northeast China.
Multi-Product Lactic Acid Bacteria Fermentations: A Review
Authors: Mora-Villalobos José Aníbal , Montero-Zamora Jéssica , Barboza Natalia , Rojas-Garbanzo Carolina , Usaga Jessie , Redondo-Solano Mauricio , Schroedter Linda , Olszewska-Widdrat Agata , López-Gómez José Pablo
Published: February 2020

Industrial biotechnology is a continuously expanding field focused on the application of microorganisms to produce chemicals using renewable sources as substrates. Currently, an increasing interest in new versatile processes, able to utilize a variety of substrates to obtain diverse products, can be observed. A robust microbial strain is critical in the creation of such processes. Lactic acid bacteria (LAB) are used to produce a wide variety of chemicals with high commercial interest. Lactic acid (LA) is the most predominant industrial product obtained from LAB fermentations, and its production is forecasted to rise as the result of the increasing demand of polylactic acid. Hence, the creation of new ways to revalorize LA production processes is of high interest and could further enhance its economic value. Therefore, this review explores some co-products of LA fermentations, derived from LAB, with special focus on bacteriocins, lipoteichoic acid, and probiotics. Finally, a multi-product process involving LA and the other compounds of interest is proposed.
A Survey on Troll Detection
Authors: Tomaiuolo Michele , Lombardo Gianfranco , Mordonini Monica , Cagnoni Stefano , Poggi Agostino
Published: February 2020

A troll is usually defined as somebody who provokes and offends people to make them angry, who wants to dominate any discussion or who tries to manipulate people’s opinions. The problems caused by such persons have increased with the diffusion of social media. Therefore, on the one hand, press bodies and magazines have begun to address the issue and to write articles about the phenomenon and its related problems while, on the other hand, universities and research centres have begun to study the features characterizing trolls and to look for solutions for their identification. This survey aims at introducing the main researches dedicated to the description of trolls and to the study and experimentation of methods for their detection.
Analysis of the Behavior of FRCM Confined Clay Brick Masonry Columns
Authors: Ombres Luciano , Verre Salvatore
Published: February 2020

The behavior of FRCM (Fabric Reinforced Cementitious Mortar) confined clay brick masonry columns is analyzed in this paper. The results of an experimental investigation conducted on small-scale columns made by clay brick masonry confined with steel-FRCM (or Steel Reinforced Grout, SRG), PBO (poly-paraphenylene-benzo-bisoxazole) FRCM and basalt-FRCM, tested under monotonic compressive load, are described and discussed. Tests were conducted on thirteen prismatic columns; eleven columns (two unconfined and nine confined) were tested under concentric load while an eccentric load was applied on two confined columns. For each confinement system, the parameters investigated were the ‘confinement ratio’, the ‘load eccentricity’ and the ‘overlap configuration of the fiber fabrics’. FRCM confinement improved the structural response of masonry columns in terms of ultimate strength, ultimate strain and ductility. Some models from the literature were also examined to evaluate their applicability in predicting the axial capacity of confined columns.
Numerical Simulations of Turbulent Combustion
Authors: Lipatnikov Andrei N.
Published: February 2020

Turbulent burning of gaseous fuels is widely used for energy conversion in stationary power generation, e [...]
Sensory and Physicochemical Analysis of Meat from Bovine Breeds in Different Livestock Production Systems, Pre-Slaughter Handling Conditions, and Ageing Time
Authors: López-Pedrouso María , Rodríguez-Vázquez Raquel , Purriños Laura , Oliván Mamen , García-Torres Susana , Sentandreu Miguel Ángel , Lorenzo José Manuel , Zapata Carlos , Franco Daniel
Published: February 2020

Different bovine breeds and production systems are used worldwide, giving rise to differences in intrinsic and extrinsic characteristics of beef. In order to meet the consumer requirements, new approaches are currently being developed to guarantee tenderness, taste, and juiciness of beef. However, the final consumer perception is complex, and it is also affected by several interrelated variables. This study aimed to evaluate the physicochemical parameters and sensory profile of three Spanish cattle breeds under different livestock production systems (extensive and intensive) and pre-slaughter handling conditions (mixing and not mixing with unfamiliar individuals at pre-mortem time). Meat samples from each group were also studied at different ageing times (7 and 14 days). Regarding sensory attributes, twelve panelists assessed meat samples and an exhaustive statistical analysis was carried out. The most evident and strongest effect was the breed type, allowing a great differentiation among them using principal components and discriminant analysis. The livestock production system was the second most important parameter, significantly affecting odor, flavor, and textural profile (fibrousness). It can be concluded that there were marked differences in the traits of these beef that could be modified by other factors in order to fulfill consumer tastes.
Exploring Consumer Palatability of Australian Beef Fajita Meat Enhanced with Phosphate or Sodium Bicarbonate
Authors: Garmyn Andrea , Hardcastle Nicholas , Bendele Clay , Polkinghorne Rod , Miller Mark
Published: February 2020

The objective of this study was to determine the consumer eating quality of five Australian beef muscles (outside skirt/diaphragm, inside skirt/transversus abdominis, inside round cap/gracilis, bottom sirloin flap/obliquus abdominis internus, and flank steak/rectus abdominis) served as fajita strips. All the muscles were divided in half and enhanced (12%) with a brine solution containing either phosphate, a “clean label” ingredient sodium bicarbonate, or not enhanced. Muscle and enhancement independently influenced (P < 0.01) tenderness, juiciness, flavor, and overall liking. Overall, the bottom sirloin flap was liked the most (P < 0.05) when compared with all the other muscles, while the inside round cap was liked less but did not differ (P > 0.05) from the inside skirt or flank steak. Samples enhanced with sodium bicarbonate were the most (P < 0.05) tender and juicy; samples enhanced with phosphate were intermediate, and the control samples were the least tender and juicy, regardless of the muscle. Flavor and overall liking were similar (P > 0.05) between clean and phosphate-enhanced samples, and both were liked more than the control samples. Enhancement was necessary for acceptable eating quality of all the muscles evaluated in this study; however, the inside round cap was the least suitable. These results indicate that a “clean label” enhanced fajita product is possible without compromising cooking yield or consumer satisfaction.
Identification of Functional Transcriptional Binding Sites within Chicken Abcg2 Gene Promoter and Screening Its Regulators
Authors: Zhang Yujuan , Huang Jinhu , Li Xiangxiu , Fang Ci , Wang Liping
Published: February 2020

Breast cancer resistance protein (BCRP), an ATP-binding cassette (ABC) half transporter encoded by the Abcg2 gene, is reported to influence the pharmacokinetics of substrate drugs during clinical therapy. The aim of this study was to clarify the mechanisms that regulate the transcription of the chicken Abcg2 gene through cloning and characterization of its promoter region. Results showed that the Abcg2 gene is transcribed by a TATA-less promoter with several putative Sp1 sites upstream from two putative CpG islands. A luciferase reporter assay conducted both in chicken leghorn male hepatoma (LMH) cells and chicken primary hepatocytes mapped a basal promoter to nucleotides −110 to +30, which is responsible for the constitutive expression of Abcg2. The 5′-region upstream of the basal promoter was characterized by both positive and negative regulatory domains. Further, using the cell-based reporter gene assay combined with RT-PCR and drug accumulation analysis, we found that four xenobiotics, daidzein, clotrimazole, ivermectin, and lipopolysaccharide (LPS), influence the expression and function of BCRP through significant regulation of the Abcg2 gene promoter. Interaction sites with the Abcg2 gene promoter of these four selected regulators were clarified by progressive deletions and mutation assays. This study shed some light on the regulatory mechanisms involved in chicken Abcg2 gene expression and the results may have far-reaching significance regarding the usage and development of veterinary drugs.
The Bacillus Subtilis K-State Promotes Stationary-Phase Mutagenesis via Oxidative Damage
Authors: Martin Holly A. , Kidman Amanda A. , Socea Jillian , Vallin Carmen , Pedraza-Reyes Mario , Robleto Eduardo A.
Published: February 2020

Bacterial cells develop mutations in the absence of cellular division through a process known as stationary-phase or stress-induced mutagenesis. This phenomenon has been studied in a few bacterial models, including Escherichia coli and Bacillus subtilis; however, the underlying mechanisms between these systems differ. For instance, RecA is not required for stationary-phase mutagenesis in B. subtilis like it is in E. coli. In B. subtilis, RecA is essential to the process of genetic transformation in the subpopulation of cells that become naturally competent in conditions of stress. Interestingly, the transcriptional regulator ComK, which controls the development of competence, does influence the accumulation of mutations in stationary phase in B. subtilis. Since recombination is not involved in this process even though ComK is, we investigated if the development of a subpopulation (K-cells) could be involved in stationary-phase mutagenesis. Using genetic knockout strains and a point-mutation reversion system, we investigated the effects of ComK, ComEA (a protein involved in DNA transport during transformation), and oxidative damage on stationary-phase mutagenesis. We found that stationary-phase revertants were more likely to have undergone the development of competence than the background of non-revertant cells, mutations accumulated independently of DNA uptake, and the presence of exogenous oxidants potentiated mutagenesis in K-cells. Therefore, the development of the K-state creates conditions favorable to an increase in the genetic diversity of the population not only through exogenous DNA uptake but also through stationary-phase mutagenesis.
Postpartum Physical Activity and Weight Retention within One Year: A Prospective Cohort Study in Vietnam
Authors: Ha Anh Vo Van , Zhao Yun , Binns Colin W. , Pham Ngoc Minh , Nguyen Phung Thi Hoang , Nguyen Cong Luat , Chu Tan Khac , Lee Andy H.
Published: February 2020

After delivery, mothers are encouraged to increase physical activity (PA) gradually to regulate body weight; however, data on PA in relation to postpartum weight retention remains scarce, particularly among Asian women. In a cohort of 1617 Vietnamese mothers, we investigated the prospective association between habitual PA exposures at 3-month postpartum and weight retention at 6-month and 12-month postpartum. Detailed information on PA intensity and domains was collected from participants using a validated instrument specifically for Vietnamese women. Linear regression analyses and a general linear model for the repeated weight retention measures were used to ascertain the apparent relationships. On average, the participants reported 3.6 (SD 3.9) and 2.6 (SD 3.8) kg weight loss at 6- and 12-month postpartum, respectively. Total and light-intensity PA were inversely associated with the postpartum weight retention (p for trend <0.05). Our findings highlight the importance of resuming PA in the early postpartum period as an appropriate weight management strategy.