bluuebunny/crossref_metadata_2025 huggingface dataset:
Physicochemical and Hydrogeological Characteristics of Water Obtained From Borehole and Rivers in Okada and Environs, Edo State, Nigeria
Authors: Ogieriakhi O.E. , Ilevbare M.
Published: March 2021

The consumption of unsafe water is detrimental to human health. It is therefore important to ascertain the quality and purity of water set out for  drinking purpose. This paper therefore investigates the physicochemical and hydrogeological properties of water obtained from boreholes and rivers located at various sites in Okada town and environs, Edo State, Nigeria. The physicochemical parameters were analyzed using standard methods. Results obtained for the physicochemical properties showed that the concentration of phosphate, sulphates, nitrates and manganese in the samples range between (0.0310-0.450) mg/L, (0.05-1.01) mg/L, (0.021-1.0) mg/L and (0.01-0.41) mg/L respectively. The maximum concentration of calcium and sodium in all the samples analyzed was 1.27 mg/L and 8.65mg/L. Comparison of the pH value of the samples shows they are all acidic in nature, with pH values which ranges from (4.3 – 6.0). In all the samples analyzed, it was observed that no trace of heavy metal (lead) was found except in Iguvinyoba River (down-stream) with a concentration of 0.001 mg/L. The biological oxygen demand (BOD) and chemical oxygen demand (COD) values from all the water samples are below the WHO standard while the dissolved oxygen (DO) values are above the WHO  standard. The results revealed that physicochemical parameters were slightly below WHO and NAFDAC standards for drinking water, except for the  high iron (Fe) content at Usen. Thus from established standard, the borehole and river water in the study area will need some treatment to render it suitable for drinking.

Keywords: physicochemical, hydrogeological properties, quality, drinking purpose, borehole and river water.
The Effects of Population Growth on Deforestation in Nigeria: 1991 – 2016
Authors: Oyetunji P.O. , Ibitoye O.S. , Akinyemi G.O. , Fadele O.A. , Oyediji O.T.
Published: March 2021

This study examines the effects of population growth on deforestation in Nigeria between 1991 and 2016. Anthropogenic factors especially population growth were identified to be the major forces responsible for deforestation in Nigeria. The Augmented Dickey Fuller (ADF) test was used to establish stationarity among the variables and the Johansen cointegration test was used to establish a long run relationship between population growth and deforestation in Nigeria. Population growth was found to have a negative effect on the available forest cover in Nigeria. The study therefore recommends the development of rural areas, enactment of policies aimed at reducing population growth and sensitization to protect the available forest resources in Nigeria.

Keywords: forests, deforestation, population growth
Body Weight Determination and Histological Examination of Livers in Normal Rats Administered with Tamsulosin
Authors: Dikko M. , Sarkingobir Y.
Published: March 2021

The objective of this study was to investigate histopathology of livers and carry out body weight determination in normal rats administered with  tamsulosin. Standard methods and procedures were used in this study. The results were revealed. Pertaining weight, at the 3rd , 6th and 8th weeks of the study, no significant difference (P>0.05) in weight was found in the group of rats treated with carvedilol (positive control), tamsulosin low dose (12μg/kg) and high dose tamsulosin (40μg/kg) compared to normal control group, respectively. Other inter-groups comparisons were not significantly different, respectively. Pertaining liver morphology, liver sections of groups revealed no significant histological lesions compared to the normal control group at the 6th and 8th weeks of the study, respectively. This study revealed that the tamsulosin cause no histopathological lesion, thus the drug might be safe to the liver and its biochemical processes.

Keywords: Tamsulosin, Liver, histopathology, weight, Wistar rats
Climate change adaptation and its implementation at national and international levels
Authors: Afinowi Olubunmi Ayodele
Published: March 2021

Climate change adaptation is the second arm of climate change action and seeks to ensure that all countries tackle the impacts of climate change by addressing both social and geographical vulnerabilities. The article discusses adaptation and its application at national and international levels. It also examines adaptation to climate change as a developmental challenge and how the principles of sustainable development can aid climate change action. The article analyses relevant aspects of national and international instruments relating to climate change adaptation and spotlights the commitment of Nigeria and South Africa to climate change adaptation. The article finds that integrating sustainable development into governance helps adaptation and increases resilience against the impacts of climate change. The article also emphasizes the necessity of good governance, particularly protection of human rights, climate finance and effective land use governance, to ensure climate change adaptation. The article argues that climate change adaptation should be viewed more as a developmental, rather than an environmental, challenge. It further asserts that coordinated action, especially in relation to land use governance and sustainable development is required to ensure sustainable climate action. This is particularly so given that citizens of many developing countries, including Nigeria and South Africa, depend on land and its resources  for social and economic development.

Keywords: Adaptation; Climate Finance; Good Governance; Nigeria; South Africa.
Advancing Online Dispute Resolution in Nigeria: Current Opportunities, Legal Challenges and the Ways Forward
Authors: Jimoh Mujib Akanni
Published: March 2021

The outbreak of COVID-19 has impacted the Nigerian legal system with the introduction of virtual court hearing. Currently, there is no legislation on virtual court hearings in Nigeria. The foregoing notwithstanding, this article examines the constitutionality of this type of hearing and its practicability under the extant laws. Virtual court had been discouraged because of the concern that it may not pass the test of public trial, which is constitutionally guaranteed. This article analyses the provisions of the Constitution as well as available case laws, which suggest that if certain requirements are met, virtual courts may pass the constitutional test of publicity of trial. It is also submitted that the virtual court will not offend the law on territorial jurisdiction. Nonetheless there are some legitimate concern about the issue of evidence, especially examination of witnesses, which may not be best suited for virtual court. Among these are technological inadequacy necessary for virtual court hearings in Nigeria leading to recommendations arising from practices in other jurisdictions.
Optimization of Crude Oil Biodegradation of Fungi Isolated from Refinery Effluent Site using Response Surface Methodology
Authors: Odili U.C. , Ibrahim F.B. , Shaibu-modagbe E.M. , Atta H.I.
Published: March 2021

The activities involved in the production and exploration of crude oil has constantly polluted the environment. This study investigated the ability of an indigenous fungus to utilize petroleum hydrocarbon. Response Surface Methodology was used to optimize the effects of pH, microbial concentration (spores/ml), and contact time (days) on the crude oil removal efficiency in refinery effluent. Monocillium sp. was isolated and used for the treatment of refinery effluent due to its predominance in the contaminated soil. Twenty experimental runs were analyzed to determine the effect of pH, microbial concentration and contact time on the oil removal efficiency. From theexperimental results obtained, a maximum oil removal efficiency of 98.42 % was achieved at a pH of 6.5, contact time of 14 days, and a microbial concentration of 3 spores/ml. The results obtained showed the percentage of crude oil removal in the effluent sample  increased with an increase in time. Optimization of the experimental result was achieved at a removal efficiency of 98.59 %, a contact time of 13.96 days, a pH of 6.85, and a microbial concentration of 3.01 spores/ml. The findings of this study revealed that Monocillium sp. is a viable hydrocarbon degrader, and can be used in the bioremediation of petroleum contaminated environments.

Keywords: Response surface, optimization, bioremediation, hydrocarbon, removal efficiency, Monocillium sp.
Appraisal of Aggregate Technical, Commercial and Collection Losses in Nigerian Electricity Distribution System
Authors: Dodo U.A. , Nwohu M. N. , Abubakar I.N. , Dodo M.A.
Published: March 2021

From a practical point of view, no power system is free of losses. Power system losses, especially in distribution systems are usually high and result in increasing the cost of operations to the electric utilities and the price tag of electricity to the consumers. Aggregate Technical, Commercial and Collection (ATC&C) losses is a reliable parameter that reveals the true energy and revenue loss conditions of distribution systems. In this paper, mathematical models were developed for the determination of billing efficiency, collection efficiency, and ATC&C losses using Life Camp Area Office’s network of Abuja Electricity Distribution Company Plc Nigeria, as a case study. The average billing efficiency, collection efficiency and ATC&C losses for the period under review were found to be 89.73%,84.80%, and 23.79% respectively. An understanding of appraisal of these losses is important to the power system Engineers, energy policy makers, and the power firms as it enables areas of high losses in the network to be identified, which will give room for credible investment plans and subsequent monitoring of the losses.

Keywords: ATC&C losses, billing, energy, distribution system, revenue
Effects of Tractorization and Organic Manure on Physical Properties of Sandy Loam Soil in Abeokuta, Nigeria
Authors: Dada P.O.O. , Musa J.J. , Olla O.O. , Ohu J.O. , Adewumi J.K.
Published: March 2021

Soil compaction has effect on soil physical properties which could affect crop growth and yield. This study was conducted to determine the influence of incorporating organic materials and load application (tractorization) on the physical properties of sandy loamy soil in Abeokuta, Ogun State, Nigeria. Organic materials were cow dung, poultry and swine manure. Application rates of organic manures were 0 (control), 5 and 10 tonnes per hectare. Forty-five plots measuring 5 by 3 m were established in a complete randomized block experimental design with three replications making a total of 135 plots. Load application was done using an MF 435 tractor coupled with a 20-disc harrow at 0 (control), 5, 10, 15 and 20 passes. Penetration resistance, bulk density, gravimetric moisture content andporosity were determined using standard procedures. Penetrometer resistance at these passes were 392.2, 293.3, 285.0, 302.0 and 224.9 kPa respectively with significant differences between treatments (P≤0.05). Mean bulk density for the passes were 1.21, 1.26, 1.31, 1.27 and 1.29 g/cm3 respectively and bulk density increased with tractor passes. The effect of tractor passes, and manure incorporation rate did not have any significant effect on gravimetric moisture content. Poultry manure increased bulk density and penetrometer resistance on plots than swine manure and cow dung hence poultry manure at 10 t/ha can be incorporated on a sandy loam soil to enhance soil fertility and sustainability.

Keywords: Tractorization, organic manure, sandy loam, penetration resistance, bulk density
Anaesthetic potency and physiological effects of refrigerated solution of thiopentone sodium in rabbits
Authors: Udegbunam RI , Udegbunam SO
Published: March 2021

Several in vitro studies have justified the use of thiopentone solutions beyond the period recommended by the manufacturer. We conducted this in vivo study to investigate the anaesthetic potency and physiological effect of refrigerated thiopentone solution. Rabbits in four groups designated A, B, C and D were anaesthetized using 25 mg/kg thiopentone solution stored for 0 (fresh solution), 3, 7 and 14 days in the refrigerator. Anaesthetic potency of the thiopentone solution was determined by monitoring the durations of anaesthesia and recovery time while its physiological effect was assessed by evaluating the changes in the heart and respiratory rates, packed cell volume and blood glucose post injections. The durations of anaesthesia and recovery time in group A were significantly (P < 0.05) longer than those obtained in groups B, C, and D. At 20 min during anaesthesia, heart rates of rabbits in group A and B were significantly (P < 0.05) lower than heart rates of rabbits in group C. Respiratory rates decreased significantly (P<0.05) in the four groups post induction of anaesthesia. Packed cell volumes (PCV) in all groups decreased significantly (P<0.05) by 10 min post induction while PCV of the groups increased at 30 and 60 min. In the four groups, no significant increase (P>0.05) in blood glucose occurred at 10 min post induction while blood glucose decreased significantly (P<0.05) at 60 min post induction. Our findings showed that the potency of thiopentone solution decreased with refrigeration. Also significant drop in blood glucose of rabbits occurred post thiopentone injection. Based on these findings, we discourage the use of refrigerated solutions of thiopentoneKeywords: Glucose, Rabbits, Refrigeration, Respiratory rate, Thiopentone sodium
Occurrence and molecular detection of avian coronavirus in selected live bird markets, northwestern, Nigeria
Authors: Bitrus I . , Shittu I. , Meseko C.A. , Joannis T.M.
Published: March 2021

Infectious bronchitis (IB) is one of the most common highly infectious viral respiratory diseases of poultry having wide geographical  distribution. Yet, little is known about the infection in the northwestern states of Nigeria. In this study, a total of 263 pooled cloacal and tracheal swab samples were collected from apparently healthy avian species (duck, dove, geese, guinea fowl, local chicken, ostrich, parrot, pigeon, peacock, and turkey). The samples were from nine live bird markets in three states (Kaduna, Kano and Jigawa) of northwestern,  Nigeria collected from September through November 2017. Total RNAs were extracted directly from the swab samples and screened for infectious bronchitis virus (IBV) using real-time reverse transcription-polymerase chain reaction. An overall prevalence of 38.0% (100/263)was recorded. IB was detected in 70 % (7/10) of the avian species with prevalence of 100 % in dove, local chicken 45.9 %, duck 42.3 %, geese 26.6 %, pigeon 23.5 %, turkey 20.0 % and guinea fowl 6.2 %. Conversely, no detection was made from ostrich, parrot, and peacock.  Widespread distribution of IBV was observed and evidence of subclinical infection in seven out of ten (70 %) of the avian species sampled. These avian species harbouring IBV may act as reservoirs with an influence on the ecology and epidemiology of the disease. Continuous surveillance and characterization of the different serotypes in avian species are recommended to inform the adoption of suitable  vaccination strategy and control measures for the disease in Nigeria.

Keywords: Infectious bronchitis virus, Live bird markets (LBMs), Molecular detection, Nigeria, Poultry
Hypolipidemic Activity of Prosopis cineraria L (Druce) Fruit Extract and Molecular Modeling Study with Farnesoid X Receptor (FXR)
Authors: Jain PG , Surana SJ
Published: March 2021

Purpose: To investigate the hypolipidemic potential of the 70 % ethanol fruit extract of Prosopis cineraria (Fabaceae) (Et. PCF) in triton-induced hyperlipidemia in rats.Methods: Et-PCF was obtained by pulverizing whole dried fruits and extracting with 70 % ethanol. Adult Sprague Dawley rats were divided into six groups of six rats each. The groups were namely normal control, hyperlipidaemic control, standard drug-treated (simvastatin 4 mg/kg), and three Et-PCF (200, 400 and 600 mg/kg, respectively)-treated groups. Apart from normal control, all other groups received a single dose of triton (200 mg/kg, i.p.) exactly 30 min after a dose of the standard drug and Et-PCF for the induction of hyperlipidemia. Twenty four hours after trito  injection, hyperlipidemia was confirmed by collecting blood samples from all the rats and testing for serum lipid profile. Antioxidant activity, in the form of inhibition of lipid peroxidation, was determined along with chromatographic analysis. Moreover, molecular docking study of â-sitosterol (active constitute of PCF) was performed with Farnesoid X receptor.Results: Triton-induced hyperlipidemia group showed significant increase in total cholesterol, low density lipoprotein cholesterol (LDL), very low density  lipoprotein cholesterol (VLDL) , triglyceride, atherogenic index and decreased high density lipoprotein cholesterol (HDL), compared to normal control group. Et-PCF treated groups showed reduction in serum cholesterol, triglyceride, VLDL and LDL levels compared to triton treated control group. Extract at the dose of 200 mg/kg significantly reduce serum cholesterol (p < 0.01) and serum LDL (p < 0.01). At the dose level of 400 mg/kg and 600 mg/kg extract is effective to significantly  reduce serum cholesterol (p < 0.05), triglyceride (p < 0.05), VLDL (p < 0.05), LDL (p < 0.05) and atherogenic index (p < 0.05) and these results are almost  equivalent to those of standard drug simvastatin. Furthermore, antioxidant activity, i.e., IC50 of Et-PCF was 58.33 ±g/ml. Molecular docking score of â-sitosterol for Farnesoid X receptor was -8.32 kcal/mol, suggesting excellent binding conformation of Et-PCF to receptor molecules.Conclusion: The findings suggest that Prosopis cineraria may be beneficial for preventing hyperlipidaemic complications by its anti-hyperlipidemic and antioxidant activities.Keywords: Prosopis cineraria, Anti-hyperlipidemic activity, Simvastatin, Triton, Docking studies, Farnesoid X receptor
Antihyperglycemic and Antioxidant Activity of Fructus hordei Germinatus Extract on Streptozotocin-induced Diabetic Rats
Authors: Wei H , Liang X , Wu B , Zhang J , Qin Y , Luo G , Luo Z
Published: March 2021

Purpose: To explore the antihyperglycemic and antioxidant effects of Fructus Hordei Germinatus extract (FHGE) in diabetic rats.Methods: The aqueous extract of FHG was obtained by steeping the dried Fructus Hordei Germinatus in water at 60oC three times, each for 1 h before first drying in an oven and then freeze-drying the last extract thus obtained. Antihyperglycemic effect was measured by blood glucose and plasma insulin levels. Oxidative stress was evaluated in liver and kidney by antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT), while blood serum levels of creatinine and urea were also determined in both diabetic control and treated rats.Results: Compared with diabetic rats, oral administration of FHGE at a concentration of 20, 40 and 80 mg/kg daily for 45 days showed a significant decrease in fasting blood glucose (p < 0.05) and increased insulin (p < 0.05) level,. Furthermore, it significantly reduced biochemical parameters (serum creatinine and serum urea, p < 0.05)). The treatment also resulted in significant increase in GSH, GPx (p < 0.05), SOD and CAT (p < 0.05), as well as decreased LPO (p < 0.05) level in the liver and kidney of diabetic rats.Conclusion: The results suggest that FHGE may effectively normalize the impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. FHGE has a protective effect against lipid peroxidation by scavenging free radicals and is thus capable of reducing the risk of diabetic complications.Keywords: Fructus hordei Germinatus, Diabetic, Antihyperglycemic, Lipid peroxidation, Antioxidant, Fasting blood glucose, Creatinine, Urea
Charge-Transfer Reaction of Cediranib with 2,3-Dichloro- 3,5-dicyano-1,4-benzoquinone: Spectrophotometric Investigation and Use in Development of Microwell Assay for Cediranib
Authors: Khalil NY , Wani TA , Darwish IA , Assiri IS
Published: March 2021

Purpose: To investigate the charge-transfer (CT) reaction between cediranib (CRB) and 2, 3 - dichloro-5, 6- dicyano -1, 4 - benzoquinone (DDQ) and employment of the reaction as a basis for the development of a novel 96 - microwell spectrophotometric assay for CRB.Method: The reaction of CRB and DDQ was investigated in different solvents of varying dielectric constant and polarity index The reaction resulted in the formation of a red-colored product. Spectrophotometric investigations confirmed that the reaction proceeded through to the formation of a (CT) complex.Results: The molar absorptivity of the CT complex was linearly correlated with the dielectric constant and polarity index of the solvent; the correlation coefficients were 0.801 and 0.858, respectively. The association constant of the complex was 0.5 × 103 L mol−1 in 2-propanol. The reaction conditions were optimized for the development of the microwell assay for CRB. The assay limits of detection and quantitation were 6.8 and 20.6 μg/well, respectively. The assay was validated as per the guidelines of the International Conference on Harmonization (ICH) and successfully applied to the analysis of CRB in its bulk and dosage forms with good accuracy and precision.Conclusion: The developed assay has high throughput and consumed minimum volume of organic solvent; thus, it reduces the exposure of the analysts to the toxic effects of organic solvents, and should significantly lower the analysis cost.Keywords: Cediranib, 2,3-dichloro-3,5-dicyano-1,4-benzoquinone, Charge-transfer reaction, Spectrophotometry, Microwell assay, High throughput analysis.
Preparation, Characterization and Evaluation of Elvitegravir-Loaded Solid Lipid Nanoparticles for Enhanced Solubility and Dissolution Rate
Authors: Kommavarapu P , Maruthapillai A , Palanisamy K
Published: March 2021

Purpose: To enhance the aqueous solubility and dissolution rate of elvitegravir (EVG) by formulating the drug as solid lipid nanoparticles (SLNs) using solvent injection method.Methods: EVG-loaded SLNs were prepared by solvent injection method. Four different formulations of SLN were prepared using gelucire - 44/14 as lipid core in ethanol, soya lecithin as emulsifier, and polysorbate 80 as surfactant in the aqueous phase. The SLNs were characterized for various physical properties, including particle size, zeta potential, polydispersity, release profile and entrapment efficiency.Results: The yield of SLNs was in the range 151.0 ± 2.4 to 199.1 ± 2.7 nm. Significant changes were observed in mean particle size (nm), Z - potential (mV) and polydispersity index (PDI) of the SLNs by varying the  concentration of cryoprotectant. EVG – SLNs demonstrated a 800 – 1030-fold enhancement in aqueous solubility compared with plain EVG. The dissolution efficiency (DE) for SLNs was > 63 % in all cases and increased up to 83 % with increasing lipid load.Conclusion: Successful preparation and characterization of elvitegravir–loaded solid lipid nanoparticles by solvent injection method has been accomplished in this study.Keywords: Elvitegravir, Solid lipid nanoparticles, Cryoprotectant, Lipid load, Entrapment efficiency
Preparation and Characterization of a Complex of Paeonol and Hydroxypropyl-β-cyclodextrin
Authors: Chen C , Xu X , Li Y , Ma H
Published: March 2021

Purpose: To improve the solubility of paeonol in water by complexing with hydroxypropyl-β-cyclodextrin (HP-β-CD) and also to characterize the physicochemical properties of the complex.Methods: The complex of paeonol and HP-β-CD was prepared by freeze-drying method. Its physicochemical properties were studied by phase-solubility method, ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), scanning electron microscopy (SEM), x-ray diffractometry (XRD) and  thermogravimetric/differential scanning calorimetry (TG/DSC).Results: The phase-solubility results showed that paeonol formed a 1:1 stoichiometric complex with HP-β-CD while UV and IR spectra suggested that HP-β-CD and paeonol in the complex interacted by a non-covalent bond. SEM and XRD indicate that the heat stability of paeonol was significantly improved by complexing with HP-β-CD.Conclusion: Paeonol can be efficiently complexed with HP-β-CD to form a complex by freeze-drying method. The aqueous solubility and heat stability of paeonol is significantly improved by complexation HP-β-CD.Keywords: Paeonol, Hydroxypropyl-β-cyclodextrin, Complexation, Aqueous solubility, Heat stability
Role of Glycol Chitosan-incorporated Ursolic Acid Nanoparticles in the Treatment of Osteosarcoma
Authors: Zhang X , Lu X , Geng W , Qu G , Zhou Z , Jiang L , Li Y , Chen X , Nie L
Published: March 2021

Purpose: To investigate the effect of ursolic acid (UA)-incorporated glycol chitosan (GC) nanoparticles on inhibition of human osteosarcoma.Methods: U2OS and Saos-2 osteosarcoma cells were transfected with ursolic acid (UA) incorporated glycol chitosan (GC) nanoparticles. Ultraviolet (UV)  spectrophotometry was used to measure drug contents in nanoparticles at 365 nm with empty GC vehicles as blank. Bicinchoninic acid assay (BCA) method was employed to determine protein concentration. Identification of apoptosis and necrosis in osteosarcoma cells was performed by propidium iodide and FITC-annexin V reagents, respectively. FAC Scan flow cytometry was used to analyse apoptotic cells.Results: Among the range of UA concentrations tested, the minimum effective concentration was 10 μM with half inhibitory concentration IC50 of 25 μM. In U2OS cells, treatment with 10 and 25 μM UAinduced apoptosis in 5.89 ± 3.90 and 60.54 ± 5.40 % cells, respectively, compared to 2.05 ± 1.01 % cells for control. In Saos-2 cells, exposure to 10 and 25 μM UA induced apoptosis in 9.86 ± 8.89 and 47.54 ± 14.5 % cells, respectively, compared to 1.79 ± 0.23 % for control cells. Western blot analysis revealed  translocation of Bax and Bcl-2 proteins from mitochondria to cell cytosol. Increase in UA concentration  from 10 μM to 25 μM led to increase in the proportion of cells in G0/G1 phase and decrease in the number of cells in S and G2/M phases. These results confirm that UA transfection arrests cell cycle in G0/G1 phase in human osteosarcoma cell lines.Conclusion: UA transfection resulted in the inhibition of cell proliferation, Ezh2 expression inhibition, and apoptosis via mitochondrial pathway due to decrease in membrane potential and release of cytochrome C, as well as cell cycle arrest in G0/G1 phase.Keywords: Osteosarcoma, Cell cycle arrest, Palliation, Glycol chitosan, Ursolic acid
Inhibitory Effect of Berberine on Zeste Homolog 2 (Ezh2) Enhancement in Human Esophageal Cell Lines
Authors: Chen S , Chen X , Li Y , Zhang F , Yang S , Mo KL , Mo X , Ding Y
Published: March 2021

Purpose: To investigate the inhibitory effect of berberine treatment on enhancement of zeste of homolog 2 (Ezh2) expressions in KYSE450 human esophageal cancer cells.Methods: Transwell motility chambers were used to analyze cell migration and invasion. Bio-Rad protein assay was used for the determination of protein concentration. Chemiluminescence with ECL system was employed for the detection of protein bands as per the manufacturer’s protocol. Staining was carried out with Alexa-Fluor 647 mouse anti-BrdU antibody. Flow cytometry was performed after adding DAPI. Annexin-V/DAPI staining and flow cytometry were used for the quantification of apoptotic cell death. Total RNA was isolated from KYSE450 cells using an RNA isolation kit.Results: Berberine-induced inhibition of Ezh2 expression led to inhibition of cell proliferation by G1 phase cell cycle arrest and induced anti-invasive properties of KYSE450 cells in Boyden chamber assays. There was 92 % reduction in invasive tendency of KYSE450 cells following treatment with berberine. Histone  methylation inhibitor, 3-deazaneoplanocin A (DZNep), also led to a similar effect on cell proliferation of KYSE450 cells. Berberine treatment also resulted in strong transcriptional reduction of the AXL receptor kinase. The results of qRT-PCR and FACS analyses showed significant inhibition of AXL mRNA and protein expression in KYSE450 carcinoma cells after treatment with berberine.Conclusion: Berberine may be an effective therapeutic agent in the treatment of esophageal carcinoma.Keywords: Berberine, Histone methylation inhibitor, Anti-invasive, Cell proliferation, Human Esophageal cancer
Characterization of Polyhydroxyalkanoates Produced by Contaminated Soil Bacteria using Wastewater and Glucose as Carbon Sources
Authors: Munir S , Jamil N
Published: March 2021

Purpose: To isolate polyhydroxyalkanoates (PHA)-producing bacterial strains from contaminated soil using industrial wastewater and glucose as carbon soured by Macrogen sequencing. Two different sources, namely, glucose and wastewater were used to ces.Methods: The strains were isolated and identified as Pseudomonas, Bacillus, Enterobacter, Exiguobacterium and Stenotrophomonas using biochemical tests and further confirmevaluate and  compare the use of wastewater as a carbon source for PHA production. The biomass obtained was analyzed by Fourier transform infra-red (FTIR) to identify the presence of PHA in it. Afterwards, PHA extraction was carried out and then gas chromatography (GC) performed to identify PHA monomers.Results: Utilization of glucose resulted in the production of PHB, while wastewater yielded copolymers poly-3 hydroxybutyrate-co-3hydroxyvalerate P(3HB-co-3HV) due to its content of volatile fatty acids such as acetic acid, propionic acid and butyric acid, which led to the production of different types of polymers. The maximum PHA production was 41 ± 0.22 % obtained for Stenotrophomonas (SM03) using 2 % glucose as carbon source while for wastewater, maximum production was achieved by the Pseudomonas strain (SM01).Conclusion: Wastewater is produced in large quantities daily during various activities and therefore can be used as a cheap carbon source for the production of valuable products such as PHA.Keywords: Polyhydroxyalkanoates, Wastewater, Glucose, Pseudomonas strain, Stenotrophomonas
Pharmacological basis for medicinal use of Ziziphyus nummularia (Rhamnaceae) leaves in gastrointestinal disorders
Authors: Hussain Sayed Muzahir , Khan Aslam , Khan Arif-ullah , Qazi Neelum Gul , Ali Fawad
Published: March 2021

Purpose: To explore the pharmacological basis for the folkloric use of Ziziphus nummularia for treating diarrhea and gastrointestinal spasm.Methods: Ziziphus nummularia crude extract (Zn.Cr) was investigated for  antidiarrheal activity (50, 100 and 300 mg/kg) in terms of reduction diarrhea  droppings as well as for antisecretory activity (300 and 1000 mg/kg) in castor-oil induced model in mice. The effect of the extract on potassium chloride (KCl, 80 mM)-induced contractions in isolated rabbit jejunum tissues were was also  examined. Furthermore, the antiulcer properties of the extract was assessed in an ethanol-induced gastrointestinal ulcer model.Results: Zn.Cr (50 – 1000 mg/kg) exhibited protective effect against castor  oil-induced diarrhea (p <0.05, p < 0.01 vs saline group) and intestinal fluid  accumulation (p < 0.001 vs. castor oil group) in mice. In isolated rabbit jejunum model, Zn.Cr concentration-dependently (0.01, 0.1, 0.3, 0.5, 1 and 3 mg/mL) caused relaxation of spontaneous and KCl-induced contractions, similar to  verapamil. Calcium antagonistic effect was indicated, as pretreatment of intestinal tissues with Zn.Cr (0.3, 0.5 and 1.0 mg/mL) produced a rightward shift in Ca2+ concentration-response curves, with suppression of maximum contraction. In ethanol-induced gastric ulceration assay, Zn.Cr (300 and 1000 mg/kg) caused52.5 and 93.6 % inhibition, respectively (p < 0.001 vs. saline group).Conclusion: These results reveal that Ziziphus nummularia possesses anti-diarrheal, anti-secretory, anti-spasmodic and anti-ulcer actions, mediated possibly through voltage-gated Ca2+channel blockade.Keywords: Ziziphus nummularia, Anti-diarrheal, Anti-secretory, Anti-spasmodic, Anti-ulcer
Effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-transferase activity in rats
Authors: Nurkhasanah Laela Hayu Nurani , Hakim Zainur Rahman
Published: March 2021

Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on  glutathione-S-trasferase (GST) activity and its hepatoprotective effect.Methods: A total of 25 rats were divided randomly into 5 groups (5 rats per group). Group I served as the baseline, group II was the negative control group, while groups III, IV and V were treated with rosella extract at doses of 10, 50 and 100 mg /kg /day, respectively for 35 days. On day 36, the animals were given a single dose of dimethyl benz(a)anthracene (DMBA) orally. After one week, blood was taken from the sinus orbitalis for measurement of serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) activities by a kinetic method with DIasys® kit. The activity of GST was measured in liver  homogenate using 1-choloro-2,4-dinitrobenzene (CDNB) method, while the  expression of GST gene was determined by reverse transcriptase polymerase chain reaction (RT-PCR).Results: Treatment with rosella extract at 10, 50 and 100 mg /kg for 35 days led to significant increasesin GST activity relative to the control group. In addition, serum SGPT and SGOT activities were significantly decreased. There were significant increases in the expression of GST gene as evidenced  by increased GST band intensity.Conclusion: These results indicate that rosella possesses significant  hepatoprotective effect against hepatic injury caused by DMBA treatment. Thus, rosella may be useful for the prevention of oxidative stress caused by free radicals produced from pollutants and foods.Keywords: Hibiscus sabdariffa, Rosella, Glutamate pyruvate transaminase (SGPT),  Glutamate oxaloacetate transaminase (SGOT), glutathion-S-transferase (GST)
Anti-hyperglycemic activity of Heliotropium strigosum (Boraginaecae) whole plant extract in alloxan-induced diabetic mice
Authors: Aslam Huma , Khan Arif-ullah , Rehman Najeeb-ur- , Ali Fawad , Nadeem Humaira , Shah Syed Majid
Published: March 2021

Purpose: To investigate Heliotropium strigosum whole plant extract for its potential to reduce the blood glucose level of alloxan-induced diabetic mice.Methods: Preliminary phytochemical analysis was carried out using standard  procedures. Diabetes was induced in Balb/c mice by injecting alloxan (200 mg/kg i.p.). The crude methanol extract of Heliotropium strigosum (Hs.Cr, 30, 100 and 300 mg/kg doses) was administered daily to alloxan-induced diabetic mice for 15 days and its effect on fasting blood glucose levels, body weight and oral glucose tolerancewas evaluated. Two control groups (non-diabetic control and diabetic control)  received normal saline (0.2 ml). Metformin (500 mg/kg) was used as reference standard.Results: Heliotropium strigosum showed positive for the presence of alkaloids, tannins and flavonoids. The extract (30, 100 and 300 mg/kg) caused significant reduction in the fasting blood glucose level of alloxan-induced diabetic mice on days 5, 10 and 15 compared to diabetic control (p < 0.001). In this regard, the  anti-hyperglycemic effect compared to the reference (metformin). The extract also timedependently decreased the body weight of the treated animals as well as improved tolerance of the oral glucose overload.Conclusion: These results indicate that Heliotropium strigosum possesses  anti-hyperglycemic effect, reduces body weight and enhances the tolerance of  glucose overload in mice. Further studies are therefore required to determine its  feasiilty as an alternate herbal medicine in the management of diabetes in humans.Keywords: Heliotropium strigosum, Anti-hyperglycemic, Alloxan-induced diabetic mice, Blood glucose, Oral glucose tolerance, Body weight
Development of a high performance liquid chromatography method for simultaneous analysis of theophylline, guaifenesin and diphenhydramine in an elixir
Authors: Hayun Yahdiana Harahap , Puspasari Maria O.
Published: March 2021

Purpose: To develop and validate a new low-cost high performance liquid  chromatography (HPLC) method for simultaneous analysis of theophylline (TH), guaifenesin (GF) and diphenhydramine hydrochloride (DH) in elixir dosage form.Methods: Chromatographic conditions were an isocratic elution with C18-Kromasil® column (250 x 4.6 mm, 5 μm), methanol-water (1:1, v/v, pH 3,0) as mobile phase, flow rate 1.0 ml/min and UV detector at λ 218 nm. The method was validated for selectivity, linearity, LOD-LOQ, precision, and accuracy.Results: Retention time of TH, GF and DH was 3.3, 5.3 and 9.1 min, respectively. The method showed good selectivity, calibration curves were linear over the concentration range of 1.000 – 10.002 μg/mL, 0.801 – 8.008 μg/mL, and 0.251 – 2.514 μg/mL (r2 > 0.999). LOD was 0.1093, 0.16520, and 0.0706 μg/mL, while LOQ was 0.3645, 0.5506, and 0.2354 μg/mL for TH, GF and DH, respectively. Recovery accuracy was 99.77 - 101.10, 100.50 - 101.95 and 99.20 - 100.13 % for TH, GF and DH, respectively; precision (RSD) was < 2.0.Conclusion: The proposed method is highly selective, sensitive, precise, and  accurate, and would suitable for the simultaneous analysis of TH, GF, and DH in elixir dosage form. Since methanol is cheaper than acetonitrile, the application of the method may reduce the cost of analysis.Keywords: Simultaneous analysis, Theophylline, Guaifenesin, Diphenhydramine hydrochloride, Elixir
In-silico design of novel myocilin inhibitors for glaucoma therapy
Authors: Tang Min , Fu Yang , Fan Ying , Fu Ming-Shui , Zheng Zhi , Xu Xun
Published: March 2021

Purpose: To explore newer computational approaches in the design of novel myocilin inhibitors for the treatment of glaucoma.Methods: An in-silico virtual screening technique based on simulation of molecular docking was utilised to design a novel myocilin inhibitors for the treatment of  glaucoma. The designed novel molecules were theoretically evaluated to predict their pharmacokinetic properties and toxic effects. Lead molecules were screened out in virtual screening technique on the basis of low binding energies obtained in AutoDock based molecular docking simulation.Results: Out of ten top lead compounds screened, ZINC01729523 and ZINC04692015 were promising, having shown potent inhibition of myocilin, good pharmacokinetic properties and absence of any toxic effects.Conclusion: In-silico virtual screening of molecular libraries containing a large number of ligands is very useful for short-listing of potential lead molecules for further structure-based discovery of antiglaucoma-drugs.Keywords: Glaucoma, Myocilin, Docking, Virtual-screening, Autodock, Ligand, Drug design
Synthesis, characterization and evaluation of biological activities of manganese-doped zinc oxide nanoparticles
Authors: Khan Shakeel Ahmad , Shahid Sammia , Bashir Waqas , Kanwal Sadia , Iqbal Ahsan
Published: March 2021

Purpose: To synthesize, characterize and investigate the antimicrobial properties of pure and manganese-doped zinc oxide nanoparticles.Method: Un-doped and manganese-doped zinc oxide (Mn-doped ZnO) nanoparticles were prepared using co-precipitation method. The synthesized Mn-doped ZnO  nanoparticles were characterized using energy-dispersive x-ray spectroscopy  (EDX), scanning electron microscopy (SEM), and x-ray diffraction (XRD)  spectroscopic techniques. Their band gap energies were measured with ultraviolet-visible (UVVis) spectroscopy, while their antioxidant properties were evaluated by ferric reducing antioxidant power (FRAP), DPPH radical-scavenging, ferric  thiocyanate (FTC) and total phenolic content (TPC) assays. The antimicrobial  activities of the nanoparticles against different bacterial strains were determined using agar diffusion method.Result: Results from XRD, SEM, EDX and UV-Vis analyses demonstrated  successful synthesis of undoped and Mn-doped ZnO nanoparticles as seen in their hexagonal, wurtzite structures. The un-doped and Mn-doped ZnO nanoparticles had average grain sizes of 16.72 nm and 17.5 nm, and band gap energies of 3.585 eV and 2.737 eV, respectively. Significant antibacterial activity was manifested by Mndoped ZnO against E. coli, S. aureus, Klebsiella and B. subtilis, with zones of inhibition (ZOIs) of 13 ± 0.09 mm, 14 ± 0.01 mm, 18 ± 0.07 mm and 20 ± 0.10 mm, respectively. The Mn-doped ZnO nanoparticles also exhibited effective and significant antioxidant potential relative to butylated hydroxytoluene (BHT) and un-doped ZnO nanoparticles.Conclusion: Mn-doped ZnO nanoparticles demonstrate significant antimicrobial and antioxidant activities. Thus, the preparation is a good candidate for further development into therapeutic formulations.Keywords: Mn-doped ZnO, Nanoparticles, Properties, Antioxidant, Antibacterial
Bacoside-A exerts protective effect against Parkinson’s disease-induced functional damage in mice via inhibition of apoptosis and oxidative response
Authors: Zhang Binbin , Shi Jiankuan , Chang Lei , Wang Hong , Wang Yaping , Li Minxia , Li Yuying , Song Yijun
Published: March 2021

Purpose: To determine the effect of bacoside-A on Parkinson's disease (PD) in a rat model, and elucidate its mechanism of action.Methods: A rat model of PD was established by administration of 5 µL of 6-hydroxydopamine in ascorbic acid (0.1 %). Measurement of serum levels of inflammatory factors was carried out using enzyme-linked immunosorbent assay (ELISA) kits. Western blotting was used to assay Bax, cytochrome c and Bcl-2 in rat hippocampus.Results: Bacoside-A treatment significantly reduced PD-induced high turning values in rats (p < 0.05). Treatment with bacoside-A reversed PD-mediated suppression of serum activities of CAT and glutathione peroxidase (GPx). In bacoside-A-treated PD rats, dose-dependent suppression of acetylcholinesterase (AChE) and inducible nitric oxide synthase (iNOS) activities were observed (p < 0.05). Bacoside-A-treated PD rats significantly (p < 0.018) reduced interleukin (IL)-1β and IL-6 levels. Treatment of PD rats with bacoside-A effectively reduced levels of tumor necrosis factor (TNF)-α, NF-κB p65, (COX)-2 and p53 protein, and also reversed up-regulations of Bax, cytochrome C, caspase-3 and caspase-9.Conclusion: Bacoside-A exhibits a protective effect against Parkinson disease-induced oxidative damage and neuronal degeneration in rats through downregulation of iNOS, AChE, inflammatory cytokines and pro-apoptotic proteins. Therefore, bacoside-A has potentials for use in the management of Parkinson disease.

Keywords: Parkinson disease, Neuroprotective, Pro-apoptotic, Cytokines, Neurotoxicity
Effect of atorvastatin on spermatogenesis in rats: A stereological study
Authors: Akdeniz Ekrem , Onger Mehmet Emin , Bolat Mustafa Suat , Firat Fatih , Gur Metin , Cinar Onder , Bakirtas Mustafa , Acıkgoz Abdullah , Erdemir Fikret
Published: March 2021

Purpose: To investigate the effects of oral atorvastatin on spermatogenesis in a rat model.Methods: Rats were equally assigned into control and study groups, the latter receiving atorvastatin (20 mg/kg/day). At the end of 12 weeks, spermatogenetic activity was evaluated using stereological and optical fractionator methods. Serum follicle-stimulating hormone (FSH), total testosterone (TT), and luteinizing hormone (LH) levels were measured using micro–ELISA kits. Total cholesterol, triglyceride (TG), low-density lipoprotein cholesterol (LDL - C), and high-density lipoprotein cholesterol levels were also measured by enzymatic colorimetric assays.Results: Testicular stereological analysis revealed that atorvastatin reduced Sertoli cell numbers (p < 0.001), spermatogonia (p < 0.001), spermatocytes (p < 0.001), and seminiferous tubule diameters (p < 0.001). LDL – C (p = 0.01) and TG (p = 0.01) values were significantly lower in the study group compared with the control group. There was no significant difference in FSH (p = 0.44), LH (p = 0.48),and TT (p = 0.06) levels between the groups.Conclusion: The findings show that atorvastatin causes deleterious effects on rat spermatogenesis. It should therefore be used with caution in clinical practice owing to its potential adverse effects, especially on male fertility.

Keywords: Statin, Atorvastatin, Spermatogenesis, Stereology, Testis
Effect of ganoderic acid on diethylnitrosamine-induced liver cancer in mice
Authors: Sun Zhongwen , Sun Libo , Li Wenwen
Published: March 2021

Purpose: To investigate the hepatoprotective role of ganoderic acid A (GAA) on liver cancer induced by diethylnitrosamine (DEN) via Nrf-2/HO-1/NF-κB signal pathway in mice.

Methods: Sixty male C57BL/6J mice were randomly divided into 4 groups: (1) control group, (2) DEN (25 mg/kg) group, (3) GAA (20 mg/kg) + DEN group, (4) GAA (40 mg/kg) + DEN group. The protective effect of GAA on liver was evaluated by determining malondialdehyde (MDA), superoxide dismutase (SOD), inflammatory cytokines including interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and the expression of heme oxygenase-1 (HO-1), nuclear factor erythroid- 2-related factor-2 (Nrf-2), IκBα, p-IκBα, p65, p-p65, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in serum.

Results: The results demonstrate that GAA treatment significantly suppressed the generation of MDA, proinflammatory cytokines, and restored the activity of SOD in the serum of DEN-induced liver cancer in mice. Western blots analysis revealed that GAA significantly restored Nrf-2/HO-1/NF-κB signal pathwayrelated protein levels in DEN-induced mice liver cancer model.

Conclusion: This research reveals the anticancer activity of GAA in liver tissue, and suggests that GAA counters DEN-induced liver  cancer through Nrf-2/HO-1/NF-κB signal pathway.

Keywords: Ganoderic acid A, Nrf-2/HO-1/NF-κB pathway, Liver cancer, MDA, GAPDH, SOD
Pharmacognostic, phytochemical, biological and spectroscopic analyses of Capparis decidua (Forsk.) Edgew root and stem bark
Authors: Wazir Muhammad Asif , Azhar Muhammad Iqbal , Mehmood Zafar Alam , Qadir Muhammad Imran , Khan M. Younis , Siddique Faheem Ahmad , Shaheer Talal , Hussain Waqar , Abbas Khizar
Published: March 2021

Purpose: To investigate the pharmacognostic, phytochemical, biological and spectroscopic analyses of Capparis decidua (Forsk.) Edgew root and stem bark.Methods: Plant material (root and stem bark) was collected, authenticated, shade-dried and extracted by maceration using methanol as a solvent separately. Powder microscopy was performed using a binocular microscope. Fluorescence, physico-chemical analysis and phytochemical screening for the presence of secondary metabolites were performed using standard methods. Brine shrimp lethalitybioassay was carried out using Artemia salina bioassay, while enzymatic modulatory study was performed by α-amylase inhibition assay. Microscopic analysis was carried out with scanning electron microscopy. Spectroscopic analysis was performed by Fourier transform infrared spectroscopy (FTIR).Results: Powder microscopy showed the presence of different cellular structures. Various colors were observed under ultraviolet (UV) and ordinary light when treated with different reagents. Phytochemical screening revealed the presence of alkaloids, tannins, saponins and flavonoids but phenol and cardiac glycosides were absent from both extracts. The root bark of the plant showed significant brine shrimp lethality activity.Conclusion: Capparis decidua (Forsk.) Edgew root and stem bark contain a variety of bioactive compounds that have medicinal and therapeutic potentials. Therefore, further investigations are required to elucidate their pharmacological properties.

Keywords: Capparis decidua (Forsk.) Edgew, Phytochemical, Spectroscopy, α-Amylase