bluuebunny/crossref_metadata_2025 huggingface dataset:
Bojungikki-Tang Augments Pembrolizumab Efficacy in Human PBMC-Injected H460 Tumor-Bearing Mice
Authors: Na Se Won , Yi Jin-Mu , Yeo Heerim , Park Sang-Min , Jeong Mibae , Chun Jaemoo , Jeong Mi-Kyung
Published: September 2024

Bojungikki-Tang (BJIKT) is traditionally used to enhance digestive function and immunity. It has gained attention as a supplement to chemotherapy or targeted therapy owing to its immune-boosting properties. This study aimed to evaluate the synergistic anti-tumor effects of BJIKT in combination with pembrolizumab in a preclinical model. MHC I/II double knockout NSG mice were humanized with peripheral blood mononuclear cells (PBMCs) and injected subcutaneously with H460 lung tumor cells to establish a humanized tumor model. Both agents were administered to evaluate their impact on tumor growth and immune cell behavior. Immunohistochemistry showed decreased exhaustion markers in CD8(+) and CD4(+) T cells within the tumor, indicating enhanced T cell activity. Additionally, RNA sequencing, transcriptome analysis, and quantitative PCR analysis were performed on tumor tissues to investigate the molecular mechanisms underlying the observed effects. The results confirmed that BJIKT improved T cell function and tumor necrosis factor signaling while suppressing transforming growth factor-β signaling. This modulation led to cell cycle arrest and apoptosis. These findings demonstrate that BJIKT, when combined with pembrolizumab, produces significant anti-tumor effects by altering immune pathways and enhancing the anti-tumor immune response. This study provides valuable insights into the role of BJIKT in the tumor microenvironment and its potential to improve therapeutic outcomes.
Development of a Machine Vision System for the Average Roughness Measurement of Shot- and Sand-Blasted Surfaces
Authors: Kim Kyungmok
Published: September 2024

This article presents a machine vision system for measuring the arithmetic average roughness of shot- and sand-blasted surfaces. In the developed system, a digital microscope was used for capturing surface images after shot- and sand-blasting processes. The captured grayscale images were analyzed with the proposed algorithm using Otsu’s global thresholding and a size bandpass filter. The algorithm detected white regions associated with the specular reflection of light on a binary image, and then calculated the size of selected regions. One-way ANOVA was used to identify the relation between the size of the regions and the arithmetic average roughness of blasted surfaces. It was noted that the average size of white regions showed a linear relation to the arithmetic average roughness of both shot- and sand-blasted surfaces. Different abrasives (shot or sand) were found to bring about differences in the rate of change of the average size within a chosen roughness range. When a surface image with unknown roughness is given, it is possible to predict the arithmetic average roughness on the basis of the relation. This machine vision system enables the fast and low-cost roughness measurement of shot- and sand-blasted surfaces. Thus, it could be useful in a quality inspection for shot- and sand-blasting.
Effect of Grinding Media Grading on Liner Wear and Load Behavior in a Ball Mill by Using Rocky DEM
Authors: Yin Zixin , Ma Daoming , Li Tongqing
Published: September 2024

The liner is a wear-prone component in ball mills, subject to continuous impacts, squeezing, and abrasion from the grinding media during operation. Its service performance directly affects the working efficiency of the ball mill. The service life of mining ball mill liners is about 8 months, and frequent downtimes occur due to liner wear and loss of effectiveness, with liner replacement accounting for about 6% of the total cost, resulting in huge economic losses. This paper focuses on a Φ305 mm × 150 mm experimental ball mill, using the discrete element software Rocky Discrete Element Method (DEM) (software version number Rocky 2022 R2) for simulation modeling analysis. With Φ10 mm and Φ20 mm mono-size particle simulations serving as reference groups, this study investigates the motion states and liner abrasion patterns under different liner heights for both sizes of grinding media in Equilibrium Quality Manufacturer (EQM) and Original Equipment Manufacturer (OEM) gradations. The results indicate that the impact of liner height on the wear of the ball mill liners is related to the size and gradation of the grinding media. The degree of liner wear from highest to lowest is as follows: EQM > Φ20 mm > OEM > Φ10 mm. Due to the effect of the cylinder end cap, the wear at the axial center of the ball mill liner is more severe than at both ends, and the wear on the facing side of the liner is more severe than on the backside. A thorough study of the influence of ball mill grinding media gradation on the wear pattern of liners is of great theoretical significance for optimizing liner structures, improving grinding efficiency, and promoting energy saving and cost reduction in ball mills. This study provides theoretical guidance for understanding the mechanisms behind liner wear in ball mills and predicting the liner lifespan.
Liftable Point-Line Configurations: Defining Equations and Irreducibility of Associated Matroid and Circuit Varieties
Authors: Clarke Oliver , Masiero Giacomo , Mohammadi Fatemeh
Published: September 2024

We study point-line configurations through the lens of projective geometry and matroid theory. Our focus is on their realization spaces, where we introduce the concepts of liftable and quasi-liftable configurations, exploring cases in which an n-tuple of collinear points can be lifted to a nondegenerate realization of a point-line configuration. We show that forest configurations are liftable and characterize the realization space of liftable configurations as the solution set of certain linear systems of equations. Moreover, we study the Zariski closure of the realization spaces of liftable and quasi-liftable configurations, known as matroid varieties, and establish their irreducibility. Additionally, we compute an irreducible decomposition for their corresponding circuit varieties. Applying these liftability properties, we present a procedure to generate some of the defining equations of the associated matroid varieties. As corollaries, we provide a geometric representation for the defining equations of two specific examples: the quadrilateral set and the 3×4 grid. While the polynomials for the latter were previously computed using specialized algorithms tailored for this configuration, the geometric interpretation of these generators was missing. We compute a minimal generating set for the corresponding ideals.
Some Properties of the Potential Field of an Almost Ricci Soliton
Authors: Blaga Adara M. , Deshmukh Sharief
Published: September 2024

In this article, we are interested in finding necessary and sufficient conditions for a compact almost Ricci soliton to be a trivial Ricci soliton. As a first result, we show that positive Ricci curvature and, for a nonzero constant c, the integral of Ric(cξ,cξ) satisfying a generic inequality on an n-dimensional compact and connected almost Ricci soliton (Mn,g,ξ,σ) are necessary and sufficient conditions for it to be isometric to the n-sphere Sn(c). As another result, we show that, if the affinity tensor of the soliton vector field ξ vanishes and the scalar curvature τ of an n-dimensional compact almost Ricci soliton (Mn,g,ξ,σ) satisfies τnσ−τ≥0, then (Mn,g,ξ,σ) is a trivial Ricci soliton. Finally, on an n-dimensional compact almost Ricci soliton (Mn,g,ξ,σ), we consider the Hodge decomposition ξ=ξ¯+∇h, where divξ¯=0, and we use the bound on the integral of Ricξ¯,ξ¯ and an integral inequality involving the scalar curvature to find another characterization of the n-sphere.
Solitons, Lumps, Breathers, and Interaction Phenomena for a (2+1)-Dimensional Variable-Coefficient Extended Shallow-Water Wave Equation
Authors: Qiu Tianwei , Wang Zhen , Yang Xiangyu , Wei Guangmei , Cui Fangsen
Published: September 2024

In this paper, soliton solutions, lump solutions, breather solutions, and lump-solitary wave solutions of a (2+1)-dimensional variable-coefficient extended shallow-water wave (vc-eSWW) equation are obtained based on its bilinear form. By calculating the vector field of the potential function, the interaction between lump waves and solitary waves is studied in detail. Lumps can emerge from the solitary wave and are semi-localized in time. The analytical solutions may enrich our understanding of the nature of shallow-water waves.
Decentralized Stochastic Recursive Gradient Method for Fully Decentralized OPF in Multi-Area Power Systems
Authors: Hussan Umair , Wang Huaizhi , Ayub Muhammad Ahsan , Rasheed Hamna , Majeed Muhammad Asghar , Peng Jianchun , Jiang Hui
Published: September 2024

This paper addresses the critical challenge of optimizing power flow in multi-area power systems while maintaining information privacy and decentralized control. The main objective is to develop a novel decentralized stochastic recursive gradient (DSRG) method for solving the optimal power flow (OPF) problem in a fully decentralized manner. Unlike traditional centralized approaches, which require extensive data sharing and centralized control, the DSRG method ensures that each area within the power system can make independent decisions based on local information while still achieving global optimization. Numerical simulations are conducted using MATLAB (Version 24.1.0.2603908) to evaluate the performance of the DSRG method on a 3-area, 9-bus test system. The results demonstrate that the DSRG method converges significantly faster than other decentralized OPF methods, reducing the overall computation time while maintaining cost efficiency and system stability. These findings highlight the DSRG method’s potential to significantly enhance the efficiency and scalability of decentralized OPF in modern power systems.
A Secure and Fair Federated Learning Framework Based on Consensus Incentive Mechanism
Authors: Zhu Feng , Hu Feng , Zhao Yanchao , Chen Bing , Tan Xiaoyang
Published: September 2024

Federated learning facilitates collaborative computation among multiple participants while safeguarding user privacy. However, current federated learning algorithms operate under the assumption that all participants are trustworthy and their systems are secure. Nonetheless, real-world scenarios present several challenges: (1) Malicious clients disrupt federated learning through model poisoning and data poisoning attacks. Although some research has proposed secure aggregation methods to address this issue, many methods have inherent limitations. (2) Clients may refuse or passively participate in the training process due to considerations of self-interest, and may even interfere with the training process due to competitive relationships. To overcome these obstacles, we have devised a reliable federated framework aimed at ensuring secure computing throughout the entirety of federated task processes. Initially, we propose a method for detecting malicious models to safeguard the integrity of model aggregation. Furthermore, we have proposed a fair contribution assessment method and awarded the right to write blocks to the creator of the optimal model, ensuring the active participation of participants in both local training and model aggregation. Finally, we establish a computational framework grounded in blockchain and smart contracts to uphold the integrity and fairness of federated tasks. To assess the efficacy of our framework, we conduct simulations involving various types of client attacks and contribution assessment scenarios using multiple open-source datasets. Results from these experiments demonstrate that our framework effectively ensures the credibility of federated tasks while achieving impartial evaluation of client contributions.
Influence of Ageing Treatment on Microstructure and Mechanical Properties of GH4169 Alloy Prepared Using Wire Arc Additive Manufacturing
Authors: You Xuewen , Song Xinli , Geng Wei , Li Zhishen
Published: September 2024

The effects of solid-solution and ageing treatments on the microstructure and mechanical properties of a GH4169 alloy made by wire arc additive manufacturing, micro-casting, and forging were researched. The microstructure, along with the size and type of precipitated phase, were analysed using an optical microscope, scanning electron microscope, and transmission electron microscope. The strength and toughness were tested using a tensile testing machine. The results show that a polygonal austenite microstructure was obtained for the GH4169 alloy prepared through wire arc additive manufacturing, micro-casting, and forging, followed by solid-solution and double-ageing treatments at different times. There were a few twins in the austenite matrix. A large number of nano-sized γ″- and γ′-precipitated phases and a small number of Laves phases and MX phases were found in the matrix. The tensile strength and yield strength of the GH4169 alloy increased first and then decreased with the ageing time. After ageing for 16 h, the maximum yield strength was 1287 ± 22 MPa, the maximum tensile strength was 1447 ± 19 MPa, and the elongation was about 19.5%. The main strength mechanism is precipitated phase strength and solid-solution strength. The fracture exhibited obvious ductile fracture characteristics.
A New Approach for Phage Cocktail Design in the Example of Anti-Mastitis Solution
Authors: Królikowska Daria , Szymańska Marta , Krzyżaniak Marta , Guziński Arkadiusz , Matusiak Rafał , Kajdanek Agnieszka , Kaczorek-Łukowska Edyta , Maszewska Agnieszka , Wójcik Ewelina A. , Dastych Jarosław
Published: September 2024

The studies on phage therapy have shown an overall protective effect of phages in bacterial infections, thus providing an optimistic outlook on the future benefits of phage-based technologies for treating bacterial diseases. However, the therapeutic effect is highly affected by the proper composition of phage cocktails. The rational approach to the design of bacteriophage cocktails, which is the subject of this study, allowed for development of an effective anti-mastitis solution, composed of virulent bacteriophages acting on Escherichia coli and Staphylococcus aureus. Based on the in-depth bioinformatic characterization of bacteriophages and their in vitro evaluation, the cocktail of five phages against E. coli and three against S. aureus strains was composed. Its testing in the milk model experiment revealed a reduction in the number of S. aureus of 45% and 30% for E. coli strains, and in the study of biofilm prevention, it demonstrated 99% inhibition of biofilm formation for all tested S. aureus strains and a minimum of 50% for 50% of E. coli strains. Such insights justify the need for rational design of cocktails for phage therapy and indicate the potential of the developed cocktail in the treatment of diseased animals, but this requires further investigations to evaluate its in vivo efficacy.
Hepatitis E and Potential Public Health Implications from a One-Health Perspective: Special Focus on the European Wild Boar (Sus scrofa)
Authors: Castagna Fabio , Liguori Giovanna , Lombardi Renato , Bava Roberto , Costagliola Anna , Giordano Antonio , Quintiliani Massimiliano , Giacomini Denise , Albergo Francesco , Gigliotti Andrea , Lupia Carmine , Ceniti Carlotta , Tilocca Bruno , Palma Ernesto , Roncada Paola , Britti Domenico
Published: September 2024

The hepatitis E virus (HEV) has become increasingly important in recent years in terms of risk for public health, as the main causative agent of acute viral hepatitis. It is a foodborne disease transmitted to humans through the consumption of contaminated water or contaminated food. Human-to-human transmission is sporadic and is linked to transfusions or transplants. The main reservoirs of the hepatitis E virus are domestic pigs and wild boars, although, compared to pigs, wild boars represent a lesser source of risk since their population is smaller and the consumption of derived products is more limited. These peculiarities often make the role of the wild boar reservoir in the spread of the disease underestimated. As a public health problem that involves several animal species and humans, the management of the disease requires an interdisciplinary approach, and the concept of “One Health” must be addressed. In this direction, the present review intends to analyze viral hepatitis E, with a particular focus on wild boar. For this purpose, literature data have been collected from different scientific search engines: PubMed, MEDLINE, and Google scholar, and several keywords such as “HEV epidemiology”, “Extrahepatic manifestations of Hepatitis E”, and “HEV infection control measures”, among others, have been used. In the first part, the manuscript provides general information on the disease, such as epidemiology, transmission methods, clinical manifestations and implications on public health. In the second part, it addresses in more detail the role of wild boar as a reservoir and the implications related to the virus epidemiology. The document will be useful to all those who intend to analyze this infectious disease from a “One-Health” perspective.
Effect of Different Basal Media and Organic Supplements on In Vitro Seedling Development of the Endangered Orchid Species Dendrobium moniliforme (L.) Swartz
Authors: Hwang Jung Eun , Park Hyeong Bin , Jeon Dae Young , Park Hwan Joon , Kim Seongjun , Lee Chang Woo , Kim Young-Joong , Yoon Young-Jun
Published: September 2024

The orchid Dendrobium moniliforme faces endangerment due to habitat loss and illegal harvesting, necessitating the development of an optimized artificial propagation system to aid conservation and reintroduction efforts. This study evaluated the effects of three plant growth media, namely Murashige and Skoog (MS), Hyponex, and Orchid Maintenance Medium (OMM) (P668), and various organic additives (apple homogenate, banana homogenate, and coconut water) on the in vitro seedling growth of D. moniliforme. The results reveal that, in early postgermination stages, seedlings achieve maximum growth in the Hyponex medium, with a fresh weight (92 mg) and root length (2.7 cm) approximately 20-fold greater than those in the MS medium and OMM. After 6 months, for seedlings grown in MS medium and OMM with banana (50 g·L−1), the mean fresh weights were 29 and 107 mg, respectively; however, the highest biomass was observed in seedlings grown in the Hyponex medium with coconut water (50 mL·L−1), exhibiting a mean fresh weight of 201 mg. This study highlights Hyponex medium with coconut water as the most effective combination for promoting D. moniliforme growth and identifies suitable organic supplements for the in vitro cultivation of seedlings from asymbiotic seed culture. This propagation system offers valuable technical support for the mass production and conservation of this epiphytic orchid.
Effects of Plant Extracts on Growth Promotion, Antioxidant Enzymes, and Secondary Metabolites in Rice (Oryza sativa) Plants
Authors: Ei Ei , Park Hyun Hwa , Kuk Yong In
Published: September 2024

Plant extracts are widely used in sustainable agriculture practices to enhance crop production and reduce chemical usage in agriculture. This study employed several extraction solutions of various plant extracts to synthesize planting and spraying strategies, assess the persistence efficacy of rice, and investigate the influence of selected water extracts on secondary chemicals at different rice planting stages. Among 17 water extracts that were evaluated on rice seeds, 7 were enhanced to align with the lengths of rice roots 50–70% and shoots 40–50%. The analysis of extraction, spraying, and planting experiments revealed that water extracts, soil application, and transplanting were the most efficient methods for stimulating rice growth, especially 0.1 and 0.5% concentrations. The efficacy of the extracts remained intact also after 14 days of treatment. This study showed that photosynthesis and antioxidant activities may play crucial roles in plant growth. Rice growth stimulation has been linked to photosynthesis, flavonoid contents, and antioxidant enzymes, providing a balanced supply of nutrients for plant growth. Among all tested water extracts, Psidium guajava, Aloe vera, Allium sativum, and Medicago sativa extracts can be used to promote plant growth in organic farming.
Combined Effects of Heavy Metal and Simulated Herbivory on Leaf Trichome Density in Sunflowers
Authors: Grossman Eyal , Shtein Ilana , Gruntman Michal
Published: September 2024

Trichomes play a key role in both heavy metal tolerance and herbivory defense, and both stressors have been shown to induce increased trichome density. However, the combined effect of these stressors on trichome density in general, and specifically on metal-hyperaccumulating plants, has yet to be examined. The aim of this study was to test the effect of cadmium availability and herbivory on leaf trichome density and herbivore deterrence in the metal hyperaccumulator Helianthus annuus. To test this, H. Annuus plants were grown in control pots or pots inoculated with 10 mg/kg cadmium and were subjected to either no herbivory or simulated herbivory using mechanical damage and foliar jasmonic acid application. Herbivore deterrence was tested in a feeding assay using Spodoptera littoralis caterpillars. Interestingly, while the trichome density of H. annuus increased by 79% or 53.5% under high cadmium availability or simulated herbivory, respectively, it decreased by 26% when the stressors were combined. Furthermore, regardless of cadmium availability, simulated herbivory induced a 40% increase in deterrence of S. littoralis. These findings suggest that the combination of metal availability and herbivory might present excessive stress to hyperaccumulators. Moreover, they suggest that the risk of metal bioaccumulation in phytoremediation can be reduced by simulated herbivory.
New Chloroplast Microsatellites in Helichrysum italicum (Roth) G. Don: Their Characterization and Application for the Evaluation of Genetic Resources
Authors: Hladnik Matjaž , Baruca Arbeiter Alenka , Gabrovšek Petra , Tomi Félix , Gibernau Marc , Brana Slavko , Bandelj Dunja
Published: September 2024

Helichrysum italicum (Roth) G. Don is a Mediterranean medicinal plant with great potential in the cosmetics, culinary and pharmaceutical fields due to its unique bioactive compounds. Its recent introduction into agroecosystems has enhanced the exploitation of genetic diversity in natural populations, although limited molecular markers have made this challenging. In the present study, primers were designed for all 43 SSRs (72.1% mononucleotide, 21% dinucleotide and 6.9% trinucleotide repeats) identified in the chloroplast genome. Populations from Cape Kamenjak (Croatia) and Corsica (France) were analyzed with ten carefully selected cpSSR markers. From the initial set of 16 cpSSRs amplified in all samples, 6 cpSSR markers were removed due to low-length polymorphisms, size homoplasy and nucleotide polymorphisms that could not be detected with allele length. Of the 38 haplotypes detected, 32 were unique to their geographic origin. The highest number of private haplotypes was observed in the Cape Kamenjak population (seven out of nine detected). Based on clustering analyses, the Kamenjak population was the most similar to the Capo Pertusato (south Corsica) population, although only one sub-haplotype was shared. Other Corsican populations were more similar to each other. A cross-species transferability test with Helichrysum litoreum Guss. and Helichrysum arenarium (L.) Moench was successfully conducted and private alleles were identified.
Response of Native and Non-Native Subarctic Plant Species to Continuous Illumination by Natural and Artificial Light
Authors: Shibaeva Tatjana G. , Sherudilo Elena G. , Rubaeva Alexandra A. , Shmakova Natalya Yu. , Titov Alexander F.
Published: September 2024

This study addressed the following questions: How does continuous lighting (CL) impact plant physiology, and photosynthetic and stress responses? Does the impact of CL depend on the source of the light and other environmental factors (natural vs. artificial)? Do responses to CL differ for native and non-native plant species in the subarctic region and, if differences exist, what physiological reasons might they be associated with them? Experiments were conducted with three plants native to the subarctic region (Geranium sylvaticum L., Geum rivale L., Potentilla erecta (L.) Raeusch.) and three non-native plant species (Geranium himalayense Klotzsch, Geum coccineum Sibth. and Sm., Potentilla atrosanguinea Loddiges ex D. Don) introduced in the Polar-Alpine Botanic Garden (KPABG, 67°38′ N). The experimental groups included three species pairs exposed to (1) a natural 16 h photoperiod, (2) natural CL, (3) an artificial 16 h photoperiod and (4) artificial CL. In the natural environment, measurements of physiological and biochemical parameters were carried out at the peak of the polar day (at the end of June), when the plants were illuminated continuously, and in the second week of August, when the day length was about 16 h. Th experiments with artificial lighting were conducted in climate chambers where plants were exposed to 16 h or 24 h photoperiods for two weeks. Other parameters (light intensity, spectrum composition, temperature and air humidity) were held constant. The obtained results have shown that plants lack specific mechanisms of tolerance to CL. The protective responses are non-specific and induced by developing photo-oxidative stress. In climate chambers, under constant environmental conditions artificial CL causes leaf injuries due to oxidative stress, the main cause of which is circadian asynchrony. In nature, plants are not photodamaged during the polar day, as endogenous rhythms are maintained due to daily fluctuations of several environmental factors (light intensity, spectral distribution, temperature and air humidity). The obtained data show that among possible non-specific protective mechanisms, plants use flavonoids to neutralize the excess ROS generated under CL. In local subarctic plants, their photoprotective role is significantly higher than in non-native introduced plant species.
Women’s Knowledge of Local Plants and Their Gastronomic Heritage in Chitral, NW Pakistan
Authors: Sabbah Arfaa , Abbasi Arshad Mehmood , Aziz Muhammad Abdul , Benhasher Fahdah Falah , Pieroni Andrea , Aldosari Ali Abdullah , Gatasheh Mansour K. , Amin Muhammad
Published: September 2024

Women are the “guardians of the kitchen” and central to household food security, yet their role has never been studied across the Hindukush region or Pamiri Knot. This study explores the women’s knowledge (specifically from the Khowar (Kho) and Wakhi linguistic groups) of local food systems and determines their role in ensuring household food security and sustainability in the mountain regions of northern Pakistan. Based on in-depth semi-structured interviews with female informants in the Rech and Broghil valleys of upper Chitral, 91 different types of food products were recorded, including wild and cultivated species. Eremurus stenophyllus and Allium barsczewskii were the commonly utilized plant species, though distinct preferences between the Kho and Wakhi groups were noted. Prominent differences were perceived in using certain cultivated plants among the two ethnic groups. For instance, Kho preferred plants like Beta vulgaris, Zea mays, and Brassica napus as indicated by the use reports, while Wakhi concentrated on Thymus serpyllum, Zygophyllum obliquum and Papaver involucratum. Both groups had shared dairy practices, but cottage cheese and curd were highly cited among Kho, while double-fermented curd and Qurut for Wakhi. The study recorded some new food uses for specific plants, such as Atriplex hortensis, Carthamus tinctorius, Hylotelephium spp., and Saxifraga sibirica. Cross-culture analyses revealed a mosaic pattern of homogenous and heterogenous trends based on reported food species of plants/animals and their use reports. Our findings emphasize the significant role of women in sustaining local food diversity, food sustainability, and the preservation and security of the local food systems, cultural legacy, and household food management. Therefore, inclusive research addressing their social, economic, and environmental issues must be conducted. Furthermore, policies must incorporate women’s traditional knowledge to build resilient food systems.
The Role of K2CO3 in the Synthesis of Dimethyl Carbonate from CO2 and Methanol
Authors: Zhou Yi , Chen Mingzhe , Wu Congyi , Dong Xueling , Yang Dezhong
Published: September 2024

The synthesis of dimethyl carbonate (DMC) from methanol and CO2 has also received widespread attention, and K2CO3 is usually used as a catalyst in the synthesis of DMC. In this work, the role of K2CO3 in synthesizing dimethyl carbonate (DMC) from methanol and CO2 was revisited. Interestingly, NMR results indicated that K2CO3 can react with methanol to form carbonate CH3OCOO−, an essential intermediate in the synthesis of DMC, which can be transformed into DMC in the presence of CH3I. In other words, K2CO3 can act as not only a catalyst but also a reactant to synthesize DMC from methanol and CO2.
Extraction of Vanadium from High Phosphorus Vanadium Containing Waste Residue via Carbonation: Optimization Using Response Surface Methodology
Authors: Bai Zhigang , Zhang Yimin , Xue Nannan , Zheng Qiushi , Dai Zilin
Published: September 2024

Vanadium (V) was successfully extracted from a high phosphorus vanadium residue (HPVR) through a carbonation process. Vanadium within HPVR substitutes for Fe in the mineral structure of Ca9(Fe,V)(PO4)7 at elevated temperatures, Na2CO3 reacts with V to form sodium metavanadate (NaVO3), concurrently generating calcium carbonate (CaCO3) through its interaction with Ca9(Fe,V)(PO4)7. Subsequently, V is liberated and leached by water, dissolving in the aqueous phase as metavanadate ions (VO3−). Crucial factors influencing V leaching efficiency include roasting time, roasting temperature, and the amount of Na2CO3 utilized. Response Surface Methodology (RSM) was employed. The optimized parameters determined were as follows: a roasting temperature of 850 °C, a roasting duration of 120 min, a Na2CO3 dosage of 8.01%, a liquid-to-solid ratio (L/S) of 3, and a leaching time of 60 min. Under these conditions, a remarkable V leaching efficiency of 83.82% was achieved. This study underscores the viability of a simplified approach for treating solid waste containing metal slag, which not only mitigates environmental pollution but also yields valuable metals.
Robustness and Scalability of Incomplete Virtual Pheromone Maps for Stigmergic Collective Exploration
Authors: Dimitrov Kaloyan , Hristov Vladimir
Published: September 2024

The Swarm Guiding and Communication System (SGCS) is a decision-making and information-sharing framework for robot swarms that only needs close-range peer-to-peer communication and no centralized control. Each robot makes decisions based on an incomplete virtual pheromone map that is updated on each interaction with another robot, imitating ant colonial behavior. Similar systems rely on continuous communication with no range limitations, environment modification, or centralized control. A computer simulation is developed to assess the effectiveness and robustness of the framework in covering an area. Consistency and the time needed for 99% coverage are compared with an unbiased random walk. The pheromone approach is shown to outperfom the unbiased one regardless of number of agents. Innate resilience to individual failures is also demonstrated.
Towards Characterization of Hass Avocado Peel and Pulp Proteome during Postharvest Shelf Life
Authors: Camacho-Vázquez Carolina , Elizalde-Contreras José Miguel , Reyes-Soria Francisco Antonio , Monribot-Villanueva Juan Luis , Guerrero-Analco José Antonio , Juarez-Escobar Janet , Velázquez-López Olinda , Meza-Menchaca Thuluz , Bojórquez-Velázquez Esaú , Zamora-Briseño Jesús Alejandro , Ramirez-Vazquez Monica , González Barrenechea Guadalupe Alheli , Ibarra-Laclette Enrique , Ruiz-May Eliel
Published: September 2024

In recent years, avocados have gained worldwide popularity as a nutritive food. This trend is causing a rise in the production of this fruit, which is accompanied by several problems associated with monocultural practices. Despite massive economic gains, limited molecular and structural information has been generated about avocado ripening. In fact, limited studies have attempted to unravel the proteome complexity dynamics of avocado fruit. We therefore conducted a comparative proteomics study on avocado peel and pulp during the postharvest shelf life using tandem mass tag synchronous precursor selection triple-stage mass spectrometry. We identified 3161 and 1128 proteins in the peel and pulp, respectively. Peels exhibited major over-accumulation of proteins associated with water deprivation and oxidative stress, along with abscisic acid biosynthesis. Ethylene, jasmonic acid, phenylpropanoid, and flavonoid biosynthesis pathways were activated. Structurally, we observed the accumulation of lignin and a reduction in cuticular thickness, which coincides with the reduction in the levels of long-chain acyl-coenzyme A synthetase and a marginal increase in 10,16-dihydroxyhexadecanoic acid. Our study sheds light on the association of proteome modulation with the structural features of Hass avocado. Its detailed characterization will provide an alternative for better preservation during the postharvest period.
The Faith at the End of Knowing
Authors: MacDonald Kevin
Published: September 2024

This essay explores the complexity of faith in our time. The continued belief in religion indicates, at least to some extent, the failure of science to fully demonstrate its absoluteness to a convincing degree for many believers. And, in Kantian terms, the reason for that is because it cannot. This essay will focus on the point at which faith and reason intersect and become neither each other nor themselves but share in an unspoken and unintended complicity of belief in order to negotiate the impossible truth of the matter, which is just that—the true is the impossible.
Self-Localization of Anonymous UGVs Using Deep Learning from Periodic Aerial Images for a GPS-Denied Environment
Authors: Poulet Olivier , Guinand Frédéric , Guérin François
Published: September 2024

This work concerns the autonomous navigation of non-holonomic ground mobile robots in a GPS-denied environment. The objective was to locate, in a global frame, without GPS, anonymous ground mobile robots starting from two consecutive aerial images captured by a single fixed webcam. The effectiveness of deep learning by a MultiLayer Perceptron in an indexed localization was compared to the methods studied in previous works. The ability of a robot to determine the position of other non-indexed robots was also performed. The structure and parameters of the network and the choice of the points taken into account during the learning phase to obtain a local optimum are presented. The results, obtained from simulated and experimental data, are compared to those obtained with more classical methods for different sampling periods (time between images).
Evaluation of the Smart Logistics Based on the SLDI Model: Evidence from China
Authors: Liu Yan , Zhao Jiaqi
Published: September 2024

Smart logistics (SL) reflects the digital transformation of the logistics industry, which is key for economic development. Most evaluations are based on the application of technology in SL, and few studies have evaluated SL from a comprehensive perspective. The paper builds the SL development index (SLDI) model from five dimensions based on the driving force, pressure, state, impact, and response (DPSIR) model and identifies the indicator weight by the entropy weight technique. The paper employs the ETDK method, a combined quantitative approach that incorporates entropy weight (E), the technique for order preference by similarity to an ideal solution (TOPSIS) (T), the Dagum Gini coefficient (D), and Kernel density estimation (K), to calculate the closeness degree, analyze spatial-temporal differentiation, and explain the distribution characteristics using data from China spanning 2013 to 2021. The findings show that (1) The SL evaluation is multidimensional and cannot be evaluated only based on technical indicators. A comprehensive evaluation indicator system is necessary. (2) A combined quantitative approach can measure SL development from multiple perspectives and get a clearer picture of the characteristics and regional differences of SL. (3) Influenced by economic development, infrastructure, regional clusters, location, talent, etc., the overall SL development is improving yearly, but SL development in different regions is unbalanced and has different distribution characteristics. The SLDI model developed in this paper will provide a more scientific and reasonable tool for comprehensively evaluating SL. The findings are helpful in proposing suggestions and optimization approaches for subsequent research on SL evaluation and development.
Sequential Immune Acquisition of Monoclonal Antibodies Enhances Phagocytosis of Acinetobacter baumannii by Recognizing ATP Synthase
Authors: Huang Dong , Zeng Zhujun , Li Zhuolin , Li Mengjun , Zhai Linlin , Lin Yuhao , Xu Rui , Qu Jiuxin , Zhang Bao , Zhao Wei , Shen Chenguang
Published: September 2024

Objectives: The aim of this study was to prepare monoclonal antibodies (mAbs) that broadly target Acinetobacter baumannii and protect against infection by multi-drug-resistant (MDR) A. baumannii from different sources. Methods: mAb 8E6 and mAb 1B5 were prepared by sequentially immunizing mice with a sublethal inoculation of three heterogeneous serotypes of pan-drug-resistant (PDR) A. baumannii, ST-208, ST-195, and ST-229. Results: The cross-recognition of heterogeneous bacteria (n = 13) by two mAbs and potential targets was verified, and the in vitro antibacterial efficacy of mAbs was assessed. The median killing rate of mAb 8E6 against A. baumannii in the presence of complement and dHL-60 cells was found to be 61.51%, while that of mAb 1B5 was 41.96%. When only dHL-60 cells were present, the killing rate of mAb 8E6 was 65.73%, while that of mAb 1B5 was 69.93%. We found that mAb 8E6 and mAb 1B5 broadly targeted MDR A. baumannii on the ATP synthase complex and were equipped with an antibacterial killing ability by enhancing the innate immune bacteriolytic effect of ST-208 and ST-195 strains. Both monoclonal antibodies were validated to protect against respiratory infection at 4 and 24 h via enhancing the release of innate immune substances and inflammatory cytokines, effectively shortening the disease period in mice. Conclusions: mAb 8E6 and mAb 1B5 significantly enhanced the opsonization process of phagocytosis against A. baumannii strains prevalent in southern China by targeting ATP synthase antigens thereof, resulting in protective effects in mice.
Microplastic: Characteristics, exposure pathways, toxicity, and implication for human health
Authors: Amrullah Muhammad Khalaf , Putri Alifa Nabilah Elma , Nababan Windika Yosephin , Siahaan Andre Marolop Pangihutan
Published: September 2024

Microplastics particles ranging from 1 µm to 5 mm, have gained attention for their omnipresence in marine environments and potential health risks. Initially recognized in the 1990s, microplastics are classified as primary (designed for microscopic dimensions) or secondary (resulting from degradation). Exposure pathways include ingestion, inhalation, and dermal contact, with sources ranging from food and beverages to personal care products. These particles possess unique properties, facilitating their interaction with organic contaminants and potential bioaccumulation in marine life. Their small size allows them to infiltrate ecosystems, raising concerns about their impacts on human health. Studies suggest associations between microplastic exposure and health issues such as inflammatory bowel disease and neurodevelopmental. Microplastics exhibit toxicity through mechanisms like oxidative stress induction and disruption of neurotransmitter levels. They have been detected in human tissues, including the brain, raising concerns about potential neurological impacts. To comprehend the effects on health over time, additional research is required, including biopersistence and tissue accumulation. Regulatory measures and consumer awareness initiatives are crucial to mitigate microplastic pollution and minimize health risks. Strategies to reduce plastic production, enhance recycling, and develop microplastic removal technologies are vital for protecting both human health and the environment. In summary, microplastics pose significant health risks due to their widespread presence and potential toxicity. Understanding their impacts and implementing effective mitigation strategies are essential for safeguarding human health and environmental integrity.
Effect of moringa flower extract on liver function and histopathology appearance of obesity model rat's liver
Authors: Wahyuniasri Nuri Rahma , Nardi Leo , Sopacua Erwin
Published: September 2024

This study aims to test and analyze the effectiveness of moringa flower extract on the liver function of male wistar strain white rats obesity model and how the histopathological picture. This research is a type of experimental quantitative research using post-test only group design. This study used test animals in the form of male white wistar rats with a body weight of 200-300 gr aged 2-3 months. The test animals were divided into 4 groups, the control group was only given regular food and distilled water, the treatment group was given high-fat food and Moringa flower extract at different doses, namely 200 mg / kgBB, 400 mg / kgBB, and 600 mg / kgBB. The results of the phytochemical test showed that moringa flower extract contains secondary metabolites in the form of saponins, tannins, flavonoids, alkaloids, and steroids. The administration of moringa flower extract at a dose of 600 mg/KgBB is effective in improving liver function in obese Wistar white rats. This improvement can be seen through the levels of ALT, AST, and histological structure of the liver that has improved. The results of histopathological observations of liver tissue in treatment group 3, namely moringa flower extract at a dose of 600 mg/KgBB, experienced the most significant improvement and approached the control group compared to other groups.
Antibacterial activity of palm leaf extract against Propionibacterium acnes and Staphylococcus aureus
Authors: Harmileni Harmileni , Hidayani Tengku Rachmi , Chiuman Linda , Marfitania Taranipa
Published: September 2024

Acne vulgaris is the most common skin disorder in adults and young adults. The growth of acne is caused by many factors, including excess sebum secretion and changes in microbial flora, mainly the colonization of Propionibacterium acnes and Staphylococcus aureus. This study aimed to determine the anti-bacterial activity of palm leaf extract against Propionibacterium acnes and Staphylococcus aureus. The research procedure includes palm leaf extraction and anti-bacterial tests. Palm leaf extraction was carried out using ultrasonication, while the anti-bacterial test was carried out using disc diffusion. The palm leaf extract obtained was diluted to 10, 20, 30, 50, and 50%, and each variation of this concentration was tested for anti-bacterial activity. The results of the anti-bacterial test showed that all palm leaf extract samples from 10% to 50% concentration could inhibit the growth of P. acnes and S. aureus bacteria. The 50% extract of ultrasonicated palm leaves had the largest inhibition zone diameter of 13.8 mm for P. acnes bacteria and 10.1 for S. aureus bacteria. From the results of this study, palm leaf extract has the potential to be used as a product for the prevention or treatment of acne vulgaris.
Demographic factors and open defecation practices: A cross-sectional study in Gunungtua Jae Village, Padang Lawas Utara
Authors: Lubis Rini Khofifah , Wisudawan B Owildan , Ahmad Haslinah
Published: September 2024

Open defecation (OD) remains a significant public health concern, particularly in rural areas of developing countries. This study aimed to investigate the association between demographic factors and OD practices in Gunungtua Jae Village, Padang Lawas Utara, Indonesia. A cross-sectional study was conducted involving 82 households. Data were collected using structured questionnaires and analyzed using Chi-square test and logistic regression. The findings revealed a significant association between several demographic factors and OD practices. These factors included household head knowledge (p = 0.000), occupation (p = 0.002), attitude (p = 0.000), education level (p = 0.000), and income level (p = 0.000). The study highlights the importance of considering demographic factors when addressing OD practices in public health interventions. Comprehensive efforts are needed to enhance community knowledge, particularly among household heads, regarding the significance of adequate sanitation and the detrimental impacts of OD practices. Collaboration between community health centers (puskesmas) and various stakeholders is essential for the sustainable implementation of the Total Sanitation Based on Community Approach (STBM) program. This program can effectively raise awareness and promote positive behavioral changes towards environmental hygiene and personal health, with a primary focus on encouraging proper sanitation practices and preventing environmental pollution.
Knowledge of primary survey on lowering consciousness among Pasar Petisah citizens
Authors: Suherman Jessen , Lubis Andriamuri Primaputra , Paramita Deryne Anggia , Nasution Afrida Aryani
Published: September 2024

Lowered consciousness is a life-threatening condition that necessitates prompt assessment and first aid. One crucial method for addressing such emergencies is the primary survey, which aims to identify and treat life-threatening conditions quickly. Pasar Petisah, one of the largest and busiest markets in Medan, is a high-risk location for such emergencies due to its crowded nature. Therefore, the community at Pasar Petisah needs to have a strong understanding of the primary survey procedure. This study is a descriptive cross-sectional analysis that uses primary data collected through questionnaires. The sample size consists of 97 subjects, selected using non-probability sampling techniques. The results indicate that among the 97 participants, only 3 (3.1%) demonstrated a good level of knowledge about the primary survey, 41 (42.3%) had a sufficient level of knowledge, and 53 (54.6%) had a lacking level of knowledge. Regarding readiness to perform the primary survey, 61 individuals (62.9%) reported feeling prepared, 23 individuals (23.7%) were doubtful, and 13 individuals (13.4%) felt unprepared. Overall, while the community at Pasar Petisah generally lacks adequate knowledge about conducting a primary survey in cases of lowered consciousness, a majority still express readiness to perform the primary survey in emergency situations.