bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative analysis of left atrial appendage closure efficacy and outcomes by CHA2DS2-VASc score group in patients with non-valvular atrial fibrillation
Authors: Zhao Mingzhong , Zhao Mengxi , Hou Cody R. , Post Felix , Herold Nora , Walsleben Jens , Yuan Qingru , Meng Zhaohui , Yu Jiangtao
Published: July 2022

Background

Higher CHA2DS2-VASc score is associated with an increased risk of adverse cardio-cerebrovascular events in patients with non-valvular atrial fibrillation (NVAF), regardless of oral anticoagulation (OAC) status. However, whether this association still exists in patients undergoing left atrial appendage closure (LAAC) is unknown. We evaluated the impact of CHA2DS2-VASc score on LAAC efficacy and outcomes.

Methods

A total of 401 consecutive patients undergoing LAAC were included and divided into 3 groups based on CHA2DS2-VASc score (0–2, 3–4, and ≥5). Baseline characteristics, periprocedural complications, and long-term outcomes were collected and compared across all groups.

Results

There were no significant differences in implantation success, periprocedural complications, and long-term outcomes across all score groups. Kaplan-Meier estimation showed that the cumulative ratio of freedom from all-cause mortality (P = 0.146), cardiovascular mortality (P = 0.519), and non-cardiovascular mortality (P = 0.168) did not differ significantly by CHA2DS2-VASc score group. LAAC decreased the risks of thromboembolism and major bleeding, resulting in a relative risk reduction (RRR) of 82.4% (P < 0.001) and 66.7% (P < 0.001) compared with expected risks in the overall cohort, respectively. Subgroup analysis indicated that observed risks of thromboembolism and major bleeding were significantly lower than the expected risks in score 3–4 and score ≥5 groups, respectively. The level of RRR increased with CHA2DS2-VASc score (P < 0.001 for trend) for thromboembolism but not for major bleeding (P = 0.2729 for trend).

Conclusion

Patients with higher CHA2DS2-VASc score did not experience worse outcomes, which may be partly attributed to more benefits provided by LAAC intervention in such patients compared to those with a low score.
Angiographic severity in acute coronary syndrome patients with and without standard modifiable risk factors
Authors: Papazoglou Andreas S. , Farmakis Ioannis T. , Zafeiropoulos Stefanos , Moysidis Dimitrios V. , Karagiannidis Efstratios , Stalikas Nikolaos , Kartas Anastasios , Stamos Konstantinos , Sofidis Georgios , Doundoulakis Ioannis , Giannopoulos Georgios , Giannakoulas George , Sianos Georgios
Published: July 2022

Background

Routine coronary artery disease (CAD) secondary prevention strategies target standard modifiable cardiovascular risk factors (SMuRFs), which include: diabetes mellitus, dyslipidemia, hypertension, and smoking. However, a significant proportion of patients with acute coronary syndrome (ACS) present without any SMuRFs. The angiographic severity of disease in this population has not yet been investigated.

Methods

After propensity score matching of patients without SMuRFs and patients with ≥1 SMuRFs (ratio 1:3), we used zero-inflated negative binomial regression modeling to investigate the relationship of SMuRF-less status with the angiographic severity of CAD, as measured by the SYNTAX score. Survival analysis was performed to investigate differences in all-cause mortality at 30 days and at the end of follow-up period.

Results

We analyzed 534 patients presenting with ACS who underwent coronary angiography. Of them, 56 (10.5%) presented without any SMuRF. After propensity score matching, the median SYNTAX score was 13.8 (IQR 0–22.1) in 56 SMuRF-less patients and 14 (IQR 5–25) in 166 patients with ≥1 SMuRFs. SMuRF-less status was associated with increased odds of zero SYNTAX score [zero-part model: odds ratio = 2.11, 95% confidence interval (CI): 1.03–4.33], but not with decreased SYNTAX score among patients with non-zero SYNTAX score (count-part model: incidence rate ratio = 0.99, 95% CI: 0.79–1.24); the overall distribution of the SYNTAX score was similar between the two groups (p = 0.26). The 30-day risk for all-cause mortality was higher for SMuRF-less patients compared to patients with ≥1 SMuRFs [hazard ratio (HR) = 3.58, 95% CI: 1.30–9.88]; however, the all-cause mortality risk was not different between the two groups over a median 1.7-year follow-up (HR = 1.72, 95% CI: 0.83–3.57).

Conclusion

Among patients with ACS, the absence of SMuRFs is associated with increased odds for non-obstructive CAD and with increased short-term mortality rates.
Novel classification for simple peripheral arteriovenous malformations based on anatomic localization: Prevalence data from the tertiary referral center in China
Authors: Shen Yuchen , Wang Zhenfeng , Yang Xitao , Zheng Lianzhou , Wen Mingzhe , Han Yifeng , Li Xiao , Zhang Liming , Wang Jingbing , You Jianxiong , Jiang Chunyu , Su Lixin , Fan Xindong , Wang Deming
Published: July 2022

Background

In absence of the large-sample study of simple peripheral arteriovenous malfomations (pAVM), we aimed to perform the epidemiological analysis of over 1,000 simple pAVM patients from our center in the past 5 years, and establish a novel classification based on the anatomical localization of the primary lesion.

Results

Between March 27, 2016, and March 31, 2021, Chinese patients who were diagnosed with simple pAVM were taken into account. Those who suffered from simple arteriovenous malformations of the central nervous system (cnsAVM), combined types of AVM, and syndromes, such as CLOVES syndrome, etc. were all excluded from this study. A total of 1,070 simple pAVM patients were screened out. All of the simple pAVM patients were diagnosed by clinical manifestations and imaging examinations. Demographic data were obtained from the National Bureau of Statistics of China. The 5-year prevalence of simple pAVM was about (2.15–6.60) /1,000,000 population. The male-female ratio was approximately 1.22:1. The pAVM inpatients that were included in the age group of 21~30 years old had the highest constituent ratio (P= 0.01). The classification included four groups: Type I (primarily occurring in soft tissue); Type II (primarily occurring in bone); Type III (primarily occurring in the viscus) and Type IV (simple pAVM coexisting with CNS lesions). There were two subtypes of Type I: the A subtype (involving one major anatomical region) and the B subtype (involving two or more major anatomical regions); two subtypes of Type II: the A subtype (the cortex was intact) and the B subtype (the lesion had broken through the cortex). Generally, 657 patients were classified as Type IA (61.4%), 232 patients were Type IB (21.7%), 82 patients were Type IIA (7.7%) and 79 were categorized as Type IIB (7.4%); the number of patients who had Type III and Type IV pAVM were 9 (0.8%) and 11 (1.0%), respectively. The clinical manifestations and diagnostic standards for each type were also systematically summarized.

Conclusions

Prevalence data for simple pAVM were analyzed, and a novel classification was proposed based on the anatomy of the lesions. The present work was expected to facilitate the diagnosis of simple pAVM in clinical works.
Five-year comparative study of thin-strut rapamycin-eluting bioabsorbable scaffold with metallic drug-eluting stent in porcine coronary artery
Authors: Liu Yaokun , Zheng Bo , Zhang Bin , Ndondo-lay Robert , Nie Fangfang , Tang Naijie , Miao Yongsheng , Li Jianping , Huo Yong
Published: July 2022

Objectives

Using quantitative coronary angiography (QCA), optical coherence tomography (OCT), histomorphometry, and pharmacokinetics, this study tried to evaluate the safety and efficacy of Biomagic rapamycin-eluting bioabsorbable scaffold (BVS) in non-atherosclerotic porcine coronary arteries.

Background

Biomagic BVS is a new generation of thin-strut bioabsorbable scaffold. We conducted comparative study detailing pathological response, safety and efficacy of Biomagic BVS and the Firebird2 rapamycin-eluting cobalt-based alloy stent (DES) in a porcine coronary artery model. The animals were followed up at 14 days, 1, 3, 6, 12, 18, 24, 30, 36, 42, 48, 54, and 60 months after stent implantation.

Methods

A total of 143 devices (95 Biomagic and 48 Firebird2) were implanted in 2 or 3 main coronary arteries of 76 nonatherosclerotic swine and examined by QCA, OCT, light microscopy, and pharmacokinetics analyses at various time points.

Results

Vascular responses to Biomagic and Firebird2 were largely comparable at all time points, with struts being sequestered within the neointima. The degree of inflammation of both devices was mild to moderate, although the Biomagic score was higher at 14 days to 24 months. However, there was no statistical difference between the two groups except 14 days. At each follow-up time point, the percentage of area stenosis in the Biomagic group was greater than that in the Firebird 2 group, but there was no statistical difference between the two groups at 3 and 12 months. The extent of fibrin deposition was similar between Biomagic and Firebird2, which peaked at 1 month and decreased rapidly thereafter. Pharmacokinetic study showed that coronary tissue sirolimus concentration remained above 2 ng/mg of tissue at 28 day. Histomorphometry showed expansile remodeling of Biomagic-implanted arteries starting after 12 months, and lumen area was significantly greater in Biomagic than Firebird2 at 36 and 42 months. These changes correlated with dismantling of Biomagic seen after 12 months. OCT images confirmed that degradation of Biomagic was complete by 36 months.

Conclusions

Biomagic demonstrates comparable long-term safety to Firebird2 in porcine coronary arteries with mild to moderate inflammation. Although Biomagic was associated with greater percent stenosis relative to Firebird2 within 36 months, expansile remodeling was observed after 12 months in Biomagic with significantly greater lumen area at ≥36 months. Scaffold resorption is considered complete at 36 months.
Intracoronary artery retrograde thrombolysis combined with percutaneous coronary interventions for ST-segment elevation myocardial infarction complicated with diabetes mellitus: A case report and literature review
Authors: Shen Mingzhi , Liao Yichao , Wang Jian , Zhou Xinger , Guo Yuting , Nong Yingqiao , Guo Yi , Lu Haihui , Jin Rongjie , Wang Jihang , Fu Zhenhong , Li Dongyun , Zhao Shihao , Tian Jinwen
Published: July 2022

Background

The management of a large thrombus burden in patients with acute myocardial infarction and diabetes is still a worldwide problem.

Case presentation

A 74-year-old Chinese woman presented with ST-segment elevation myocardial infarction (STEMI) complicated with diabetes mellitus and hypertension. Angiography revealed massive thrombus formation in the mid-segment of the right coronary artery leading to vascular occlusion. The sheared balloon was placed far from the occlusion segment and urokinase (100,000 u) was administered for intracoronary artery retrograde thrombolysis, and thrombolysis in myocardial infarction (TIMI) grade 3 blood flow was restored within 7 min. At last, one stent was accurately implanted into the culprit’s vessel. No-reflow, coronary slow flow, and reperfusion arrhythmia were not observed during this process.

Conclusion

Intracoronary artery retrograde thrombolysis (ICART) can be effectively and safely used in patients with STEMI along with diabetes mellitus and hypertension, even if the myocardial infarction exceeds 12 h (REST or named ICART ClinicalTrials.gov number, ChiCTR1900023849).
Boosting Face Presentation Attack Detection in Multi-Spectral Videos Through Score Fusion of Wavelet Partition Images
Authors: Agarwal Akshay , Singh Richa , Vatsa Mayank , Noore Afzel
Published: July 2022

Presentation attack detection (PAD) algorithms have become an integral requirement for the secure usage of face recognition systems. As face recognition algorithms and applications increase from constrained to unconstrained environments and in multispectral scenarios, presentation attack detection algorithms must also increase their scope and effectiveness. It is important to realize that the PAD algorithms are not only effective for one environment or condition but rather be generalizable to a multitude of variabilities that are presented to a face recognition algorithm. With this motivation, as the first contribution, the article presents a unified PAD algorithm for different kinds of attacks such as printed photos, a replay of video, 3D masks, silicone masks, and wax faces. The proposed algorithm utilizes a combination of wavelet decomposed raw input images from sensor and face region data to detect whether the input image is bonafide or attacked. The second contribution of the article is the collection of a large presentation attack database in the NIR spectrum, containing images from individuals of two ethnicities. The database contains 500 print attack videos which comprise approximately 1,00,000 frames collectively in the NIR spectrum. Extensive evaluation of the algorithm on NIR images as well as visible spectrum images obtained from existing benchmark databases shows that the proposed algorithm yields state-of-the-art results and surpassed several complex and state-of-the-art algorithms. For instance, on benchmark datasets, namely CASIA-FASD, Replay-Attack, and MSU-MFSD, the proposed algorithm achieves a maximum error of 0.92% which is significantly lower than state-of-the-art attack detection algorithms.
Isotopic and Hydrogeochemical Characteristics and Genesis of Warm Springs in the Jiangcang Basin, Qinghai, China
Authors: Liu Lingxia , Qi Shihua , Zhang Yongbin , Gao Ming , Hu Jing , Wang Wenzhong
Published: July 2022

The Jiangcang Basin is a Cenozoic faulted basin, where many low-temperature warm springs are exposed, with the highest temperature being 36.6°C. The study of the hydrogeochemical characteristics of the geothermal fluids of these warm springs can effectively clarify the genetic mechanisms of the geothermal resources in the Jiangcang Basin and thus is of great significance for the development and utilization of geothermal resources in the Qinghai Province. Using the geothermal and geological conditions of the Jiangcang Basin, this study analyzed the hydrochemical and isotopic characteristics of water samples collected from cold water and from four warm springs in the Jiangcang Basin, obtaining the following results. The four warm springs have the hydrochemical types of HCO3-Ca·Mg, HCO3-Ca·Na·Mg, and HCO3·SO4-Na·Ca and total dissolved solids (TDSs) of 720.4–1,663 mg/L. Moreover, no significant positive correlation is found between Cl− and other ions in the warm springs. The results of isotopic analysis show that the warm springs are mainly recharged by atmospheric precipitation and meltwater from ice and snow, with a recharge elevation of 4,401–4,501 m. Furthermore, the geothermal reservoirs in the Jiangcang Basin have temperatures of 51–83°C and circulation depths of 1,848–2,610 m. Atmospheric precipitation and the meltwater from ice and snow infiltrate downward in high mountain areas and form runoffs along the tectonic channels in the basin. After being heated through deep circulation, they migrate upward along fault fracture zones under the influence of faults and the water blocking of mudstones, during which they cool by thermal conduction and mixing with shallow cold groundwater. Finally, the mixed water is exposed in the form of low-temperature tectonic warm springs. The results of this study can provide data for the exploitation and utilization of geothermal resources in the Qinghai Province.
Spatial and Temporal Evolution Characteristics of Low Water Level Based on 70 years’ Measured Data: A Case Study of Dongting Lake in China
Authors: Ge Hua , Zhu Lingling , Long Rui , Deng Chunyan , Yin Shuran , Zhang Ouyang
Published: July 2022

In recent years, the operation of cascade reservoirs in the upper reaches of the Yangtze River led by the Three Gorges Reservoir has significantly changed the low water level of Dongting Lake, which has had an adverse impact on the water resources and ecological security in the lake area. As a result, the low water level situation of Dongting Lake is drawing more and more attention. Based on the abundant and long-term daily observation data of Dongting Lake and main stream of the Yangtze River, the spatial and temporal evolution characteristics of low water level in the Dongting Lake area since the 1950s were analyzed in this study, especially during the storage period of the Three Gorges Reservoir. In addition, the influence of the jacking effect of the Yangtze River on Dongting Lake was also discussed to analyze the impact of the Three Gorges Reservoir. The results show that the annual minimum water level in the Dongting Lake area over the years generally occurs from January to February and December. Since it is not within the storage period of the Three Gorges Reservoir, it is not affected by the Three Gorges Reservoir. However, the average water level in September and October during the main storage period of the Three Gorges Reservoir has significantly reduced, and the closer it is to the main stream of the Yangtze River, the greater the influence is. In recent years, affected by the advance of the impounding period, the average water level has decreased further in September. In addition, the low water level of Dongting Lake is obviously affected by the jacking effect of the main stream of the Yangtze River. After the impoundment of the Three Gorges Reservoir, the water surface slope of the Dongting Lake area has decreased, while that of the main stream of the Yangtze River has increased. This has strengthened the jacking effect of the main stream. The research results can provide reference for the solution of water resource and ecological crises in the Dongting Lake area.
Mapping and assessing ecosystem service supply–demand to identify critical areas: A case study of a waterside area in Shanghai metropolitan area
Authors: Ni Yuchun , Wei Jiaxing , Wu Tao , Guo Min , Han Qiyao , Zhang Mingjuan
Published: July 2022

In the past decades, rapid urbanization in China has dramatically transformed natural spaces into construction land, leading to serious degradation and supply–demand imbalance of ecosystem services (ESs). The identification of critical areas and ecological security patterns is crucial for balancing ESs and improving human well-being in rapidly urbanized regions. The purpose of this study was to establish a comprehensive assessment framework of ES supply–demand including provisioning, regulating, supporting, and cultural services, so as to provide theoretical support for the identification and refined management of regional critical areas. Taking a typical waterside area in Shanghai metropolitan area as an example, based on multi-source data and ES quantitative models, we used ecological supply–demand ratio and bivariate local indicators of spatial association to quantify the relationship and matching patterns of ES supply–demand, then explored the identification, protection, and restoration of ecologically critical areas at the regional scale. The results showed that: 1) the ES supply–demand relationship in the study area was quantitatively determined. The ecological supply–demand ratio was as follows: regulating > provisioning > supporting > cultural, in which the supply of supporting and cultural services was less than the demand, and the problem of the supply–demand mismatch is prominent; 2) we have identified 41 supply critical areas that require priority protection, with a total area of 206.79 km2 accounting for 9.65% of the total study area, showing a spatial pattern of more in the northwest and less in the southeast; 3) a total of 11 demand critical areas that need ecological restoration were identified, accounting for 31.43% of the 35 administrative towns in the study area, which are mainly distributed around three urban centers and a high-tech zone. The study is of great significance for the construction of regional ecological security patterns and rational ES allocation, and can provide a scientific framework for the ecological protection and restoration of critical areas around metropolises in developing countries.
Active crustal deformation model of the Fen–Wei rift zone, North China: Integration of geologic, geodetic, and stress direction datasets
Authors: Song Shangwu , Li Yuhang , Hao Ming
Published: July 2022

The Fen–Wei rift zone (FWRZ) of North China is an important zone of active crustal deformation representing a transition from extrusion tectonics related to the Tibetan Plateau to subduction tectonics related to the potential far-field influence of the west Pacific plate. In this study, we determined the kinematic constraints of active crustal deformation in the FWRZ, which are fundamental for forecasting seismicity. NeoKinema, a kinematic finite-element model, was employed to estimate the long-term fault slip rates, distributed crustal deformation field, and on- and off-fault strain-rate fields in the FWRZ by fitting updated geological fault slip rate, geodetic GPS velocity, and principal compressive stress direction datasets. Our results show that the FWRZ is a characteristic low-strain kinematic setting, with most active faults exhibiting slip rates of less than 1 mm/a. The total sinistral shear rate from the southern Ordos block to the Qinling Mountains is approximately 1 mm/a, indicating limited tectonic extrusion along the EW-trending Qinling Mountains. Additionally, the central Shanxi rift exhibits prominent dextral shear of ∼0.5 mm/a that decreases toward its north and south ends, corresponding to crustal extension of 1.1–1.2 mm/a in the Datong and Yuncheng basins, respectively. However, this significant crustal extension cannot be solely attributed to terminal effects caused by dextral shear in the central Shanxi rift. A comparison between predicted seismicity and historical earthquake records reveals some remarkable seismic gaps, particularly in the Datong, Hancheng, and Yuncheng basins, indicating higher seismic potential in these locations. This study provides insights into the long-term crustal deformation processes and regional seismic potential of the FWRZ.
The role of positive atmosphere on learning generic skills in higher education—Experiences of physical education students
Authors: Virtanen Anne , Lauritsalo Kirsti , Mäkinen Tommi , Hurskainen Heikki , Tynjälä Päivi
Published: July 2022

The purpose of this study is to examine what kind of role does positive atmosphere play in learning generic skills. The study was carried out in the final year of Finnish physical education (PE) teacher education where teachers of this study module have developed their pedagogical practices for a long time to foster and maintain the positive learning atmosphere of the course. In this study the learning atmosphere was examined from the perspectives of students addressing the following research questions: (1) How do the students perceive and experience the constructed learning atmosphere? (2) What generic skills do PE students report to learn during the final year study module? (3) What kind of relationship exists between the perceived learning atmosphere and learning generic skills? The data of this study were collected via an internet questionnaire (N = 189, n = 125) and interviews (n = 19) and analyzed applying qualitative content analysis and using statistical methods. According to the results, the PE students felt that the learning atmosphere of their final year study module was warm and conversational; it was easy to get their own voice heard during the final year. The PE students reported that they had learned versatile and plenty of different generic skills, particularly various social skills. There were statistically significant associations between positive atmosphere and some generic skills, such as the development of creativity, but they were not very strong. To understand this finding, the instruments of the study are discussed, especially from the perspective of the development of the questionnaire statements. Although the relationship between perceived positive atmosphere and learning generic skills was only moderate, the findings are promising. For example, the good practices documented in this study, such as how to construct a warm educational atmosphere, may be applied when developing other study modules.
Snailed It! Inside the Shell: Using Augmented Reality as a Window Into Biodiversity
Authors: Merino Cristian , Iturbe-Sarunic Catalina , Miller Brant G. , Parent Christine E. , Phillips John G. , Pino Sonia , Garrido José Miguel , Arenas Andoni , Zamora Juan
Published: July 2022

Snails have occupied an important role in the ideology and religion of the ancient American peoples, who considered them to be magical and used them in ritual ceremonies as ornaments, musical instruments, and architectural elements. Today, they are a valuable study system for understanding biodiversity and evolution due to their remarkable ecological and morphological diversity. Given that many endemic snails are of conservation concern, and that most South American species are poorly studied, there is a need to engage the public through understandable and scientifically based language, conveying the importance of biodiversity. However, not all biodiversity can be seen with the naked eye. Herein, we describe how we utilize snails and their shells to engage citizens and train teachers to promote the many different facets of biodiversity. Through design-based research oriented toward educational innovation, we created a teaching–learning sequence with immersive technology through the following stages of work: (1) produce a teaching–learning sequence and accompanying mobile device application (for Android on GooglePlay), (2) evaluate the impact of the educational resource, and (3) conduct research through a pre- and posttest design on the learning outcomes of participants. In this work, we first present the field experience where scientists, teachers, and pre-service teachers worked together to find snails from northern Chile to Chiloé Island. Some results from this research stage are: criteria for designing a teaching–learning sequence (e.g., how to utilize place as an opportunity for learning science with developmentally appropriate technologies identified for every phase of the sequence), modeling relevant phenomena about biodiversity and ecosystems through snails, scaffolding for teachers implementing the sequence, and activities that enhance STEM education. A teaching–learning sequence that addresses snails as study objects for 4th grade is presented and validated, allowing us to continue the next phase of our research with schools. A second article will propose results from implementation, iterations, and their implications.
Oscillation-Based Spectroscopy for Cell-Culture Monitorization
Authors: Pérez Pablo , Serrano-Viseas Juan A. , Fernández-Scagliusi Santiago , Martín-Fernández Daniel , Huertas Gloria , Yúfera Alberto
Published: July 2022

Biological Impedance is a physical property related to the state and inherent evolution of biological samples. Among the existing impedance measurement methods, Oscillation-Based (OB) tests are a simple and smart solution to indirectly measure impedance correlated with the amplitude and frequency of the generated oscillation which are proportional to the sample under test. An OB test requires tuning of the system blocks to specifications derived from every measurement problem. The OB setup must be done to obtain the optimum measurement sensitivity for the specific constraints imposed by the system under test, electronic interfaces, and electrodes employed for test. This work proposes the extension of OB measurement systems to spectroscopy test, enabling a completely new range of applications for this technology without the restrictions imposed by setting a fixed frequency on the electrical oscillator. Some examples will be presented to the measurement of cell cultures samples, considering the corresponding circuit interfaces and electric models for the electrode-cell system. The proposed analysis method allows the selection of the best oscillator elements for optimum sensitivity range in amplitude and frequency oscillation values, when a specific cell culture is monitored for the OB system.
TAC3/TACR3 System Function in the Catadromous Migration Teleost, Anguilla japonica
Authors: Zuo Chenpeng , Lyu Likang , Zou Wenhui , Wen Haishen , Li Yun , Qi Xin
Published: July 2022

Neurokinin B (NKB), a member of the tachykinin (TAC) family, plays important roles in mammalian neuropeptide secretion in related to reproduction. However, its potential role in spawning migration teleost is less clear. In the present study, Japanese eel (Anguilla japonica) was employed to study the performance of NKB in regulating reproduction. Results showed that two tac3 and one tacr3 genes were identified in Japanese eel. Sequence analysis showed that two tac3 transcripts, tac3a and tac3b, encode four NKBs: NKBa-13, NKBa-10, NKBb-13, and NKBb-10. However, compared with other species, a mutation caused early termination of TACR3 protein was confirmed, leading to the loss of the 35 amino acid (aa) C-terminal of the receptor. Expression analysis in different tissues showed that both tac3a and tac3b mRNAs were highly expressed in the brain. In situ hybridization localized both tac3a and tac3b mRNAs to several brain regions, mainly in the telencephalon and hypothalamus. Because of the mutation in TACR3 of Japanese eel, we further analyzed whether it could activate the downstream signaling pathway. Luciferase assay results showed the negative regulation of cAMP Response Element (CRE) and Sterol Response Element (SRE) signal pathways by Japanese eel NKBs. Intraperitoneal injection of four different NKB mature peptides at 100 ng/g had negative effect on either gnrh or gth gene expression. However, the high concentration of NKBa-10 and NKBb-13 (1,000 ng/g) upregulated mgnrh and fshb or lhb expression level significantly, which may be mediated by other receptors. In general, the NKBs/NK3Rs system has important functions in regulating eel puberty onset.
Recurrent Implantation Failure May Be Identified by a Combination of Diagnostic Biomarkers: An Analysis of Peripheral Blood Lymphocyte Subsets
Authors: Cai Jun-Ying , Tang Yuan-Yuan , Deng Xi-He , Li Yan-Juan , Liang Gui , Meng Ya-Qing , Zhou Hong
Published: July 2022

Background

Recurrent implantation failure (RIF) is a challenge during assisted reproductive technology (ART). In the present study, potential diagnostic biomarkers for the immune status of peripheral blood lymphocyte subsets in patients with RIF were analyzed, with the aim of identifying novel biomarkers that may predict RIF.

Methods

A total of 41 participants, including 21 women with RIF and 20 fertile controls, were included in the present study. Functional analysis was performed and the cytokine status of natural killer (NK), T, CD8+ T, T helper (Th), and γδ T cells which are lymphocyte subsets in peripheral blood was measured using flow cytometry. Binary logistic regression analysis adjusted for T follicular helper 1 (Tfh1), Tfh2, Tfh17, and early NK cells was performed to determine the relationship between the peripheral blood lymphocyte subsets and RIF. Potential diagnostic biomarkers were assessed by logistic regression analysis and receiver operating characteristic curves.

Results

There were significantly more Tfh1, Tfh17, and NK cells in the RIF group compared with the control group (all P < 0.05). However, the percentage of T, regulatory T (Tregs), and Tfh2 cells, as well as early inhibitory NK cells, was significantly lower in the RIF group compared with the control group (all P < 0.05). Following logistics regression analysis, Treg, Tfh17, and early inhibitory NK cells exhibited significant differences between the two groups. Combination diagnosis using these 3 biomarkers had a higher area under the curve of 0.900 (95% confidence interval: 0.808–0.992, P < 0.001) in the RIF group compared with that in the control group.

Conclusion

T, Tregs, Tfh1, Tfh2, Tfh17, NK cells, and early inhibitory NK cells may play important regulatory roles in embryo implantation. The combination of 3 molecular markers (Treg, Tfh17, and early inhibitory NK cells) could provide a high diagnostic value for women with RIF, thus providing novel potential biomarkers for RIF in ART. The present findings could provide a reference either for the clinical treatment of patients with RIF or for future large, well-designed studies.
Association between height growth patterns in puberty and stature in late adolescence: A longitudinal analysis in chinese children and adolescents from 2006 to 2016
Authors: Chen Li , Su Binbin , Zhang Yi , Ma Tao , Liu Jieyu , Yang Zhaogeng , Li Yanhui , Gao Di , Chen Manman , Ma Ying , Wang Xijie , Wen Bo , Jiang Jun , Dong Yanhui , Song Yi , Ma Jun
Published: July 2022

Introduction

The relationship between the characteristics of puberty growth and the stature (height and overweight and obesity) in late adolescence was not clear. We aimed to explore the effects of puberty growth patterns on the stature in late adolescence.

Methods

A total of 13,143 children from a longitudinal cohort from 2006 to 2016 in Zhongshan city of China were included. The Preece–Baines growth curve was fitted for each individual child, and the age at peak height velocity (APHV), peak height velocity (PHV), and age at take-off (TOA) were obtained from the Preece–Baines model. To compare the difference in height in late adolescence (at 18 years old) at different pubertal height growth patterns (height spurt timing, intensity, and duration), the height at baseline was matched by using the propensity score matching. The log-binomial model was applied to assess the association between the three pubertal height growth patterns (timing, intensity, and duration) and overweight and obesity status in late adolescence, controlling the urbanity and body mass index (BMI) at baseline.

Results

After matching the baseline height, boys and girls in three pubertal patterns with early timing (P < 0.01), small intensity (P < 0.01), and short duration (P < 0.01) of height spurt had the lowest final height in the late adolescence. A 16% increase and 45% increase of risk for overweight and obesity were significantly associated with the early APHV in boys and girls, respectively, relative risk (RR) in boys, 1.16(95% confidence interval, CI: 1.03–1.30), P = 0.011; RR in girls, 1.45(1.21–1.75), P < 0.001. A 21% increase and 59% increase of risk for overweight and obesity were significantly associated with small PHV in boys and girls, respectively, RR in boys, 1.21(1.07–1.36), P < 0.001; RR in girls, 1.59(1.30–1.95), P < 0.001; and an 80% increase of risk for overweight and obesity with small spurt duration in girls (RR = 1.80; 95% CI: 1.49, 2.18; P < 0.001).

Conclusion

Pubertal growth patterns, including earlier puberty onset timing, smaller puberty intensity, and shorter puberty spurt duration, had a positive association with lower height risks and higher overweight and obesity risks in late adolescence.
Aging Relevant Metabolite Itaconate Inhibits Inflammatory Bone Loss
Authors: Wang Yuting , Li Song , Zhao Liming , Cheng Peng , Liu Jian , Guo Fengjing , Xiao Jun , Zhu Wentao , Chen Anmin
Published: July 2022

Progressive bone loss during aging makes osteoporosis one of the most common and life impacting conditions in geriatric populations. The bone homeostasis is maintained through persistent remodeling mediated by bone-forming osteoblast and bone-resorbing osteoclast. Inflammaging, a condition characterized by increased pro-inflammatory markers in the blood and other tissues during aging, has been reported to be associated with skeletal stem/progenitor cell dysfunction, which will result in impaired bone formation. However, the role of age-related inflammation and metabolites in regulation of osteoclast remains largely unknown. In the present study, we observed dichotomous phenotypes of anti-inflammatory metabolite itaconate in responding to inflammaging. Itaconate is upregulated in macrophages during aging but has less reactivity in responding to RANKL stimulation in aged macrophages. We confirmed the inhibitory effect of itaconate in regulating osteoclast differentiation and activation, and further verified the rescue role of itaconate in lipopolysaccharides induced inflammatory bone loss animal model. Our findings revealed that itaconate is a crucial regulatory metabolite during inflammaging that inhibits osteoclast to maintain bone homeostasis.
Development of an Active Surveillance or Surgery Model to Predict Lymph Node Metastasis in cN0 Papillary Thyroid Microcarcinoma
Authors: Zhang Huan , Zheng Xiangqian , Liu Juntian , Gao Ming , Qian Biyun
Published: July 2022

Objective

Involvement of multiple lymph node (LN) metastasis in papillary thyroid microcarcinoma (PTMC) may indicate a progressive disease. To assist treatment decision, we conducted a clinical study to develop and validate a prediction model for the preoperative evaluation of LN metastasis involving more than five lymph nodes in patients with clinical N0 (cN0) PTMC.

Material and Methods

Using data from 6,337 patients with cN0 PTMCs at Tianjin Medical University Cancer Institute and Hospital from 2013 to 2017, we identified and integrated risk factors for the prediction of multiple LN metastasis to build a nomogram. The predictive accuracy and discriminative ability of the nomogram were evaluated by the concordance index (C-index) and calibration curve. The model was validated using bootstrap resampling of the training cohort and an independent temporal validation cohort at the same institution.

Results

In the training cohort (n = 3,209 patients), six independent risk factors were identified and included the prediction model (PTMC Active Surveillance or Surgery (ASOS) Model), including age, gender, multifocality, tumor size, calcification, and aspect ratio. The PTMC ASOS model was validated both internally and through the temporal validation cohort (n = 3,128 patients) from the same institute. The C-indexes of the prediction model in the training cohort were 0.768 (95% CI, 0.698–0.838), 0.768 and 0.771 in the internal validation and external validation cohorts, respectively. The area under the receiver operating characteristic curve (AUC) was 0.7068 and 0.6799. The calibration curve for probability of large-LN metastasis showed good agreement between prediction by nomogram and actual observation. DCA curves were used for comparison with another model, and IDI and NRI were also calculated. The cutoff value of our model was obtained by the ROC curve. Based on this model and cut point, a web-based dynamic nomogram was developed (https://tjmuch-thyroid.shinyapps.io/PTMCASOSM/).

Conclusion

We established a novel nomogram that can help to distinguish preoperatively cN0 PTMC patients with or without metastasis of multiple lymph nodes. This clinical prediction model may be used in decision making for both active surveillance and surgery.
Multi-System Langerhans Cell Histiocytosis as a Mimic of IgG4-Related Disease: A Case Report and Literature Review
Authors: Feng Xiaohui , Zhang Lu , Chen Fuqiong , Yuan Gang
Published: July 2022

Langerhans cell histiocytosis (LCH) is a rare disease characterized by the clonal accumulation and/or proliferation of specific dendritic cells resembling normal epidermal Langerhans cells (LCs). Clinical manifestations are variable, depending on the affected tissues or organs, however, LCH with elevated serum IgG4 has not been reported. Herein, we reported a 26-year-old Chinese female multi-system LCH (MS-LCH) who first presented with central diabetes insipidus (CDI), accompanied by panhypopituitarism and hepatic dysfunction. Diagnostic investigations were strongly suspicious of IgG4-RD because of elevated serum IgG4 levels during the process. Furtherly, thyroid and lymph node involvement and biopsy led to the diagnosis of MS-LCH; the strongly positive staining of CD1a, S100, CD207 (langerin), and Ki67 was found. Moreover, after systemic treatment with five cycles of chemotherapy, many lesions were greatly improved. Since both LCH and IgG4-RD are orphan diseases that can affect any organ, the differential diagnosis is challenging, especially when LCH is associated with unexplained serum IgG4 elevation. In this article, the case of a young woman suffering from MS-LCH that affected organs including the pituitary, thyroid, lymph node, and liver was summarized, and relevant literature was reviewed to better equip the diagnosis and treatment in its early stages.
NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation
Authors: Lin Jiayu , Xiang Yuting , Huang Jiana , Zeng Haitao , Zeng Yanyan , Liu Jiawen , Wu Taibao , Liang Qiqi , Liang Xiaoyan , Li Jingjie , Zhou Chuanchuan
Published: July 2022

In vitro maturation (IVM) refers to the process of developing immature oocytes into the mature in vitro under the microenvironment analogous to follicle fluid. It is an important technique for patients with polycystic ovary syndrome and, especially, those young patients with the need of fertility preservation. However, as the mechanisms of oocyte maturation have not been fully understood yet, the cultivation efficiency of IVM is not satisfactory. It was confirmed in our previous study that oocyte maturation was impaired after N-acetyltransferase 10 (NAT10) knockdown (KD). In the present study, we further explored the transcriptome alteration of NAT10-depleted oocytes and found that O-GlcNAcase(OGA) was an important target gene for NAT10-mediated ac4C modification in oocyte maturation. NAT10 might regulate OGA stability and expression by suppressing its degradation. To find out whether the influence of NAT10-mediated ac4C on oocyte maturation was mediated by OGA, we further explored the role of OGA in IVM. After knocking down OGA of oocytes, oocyte maturation was inhibited. In addition, as oocytes matured, OGA expression increased and, conversely, O-linked N-acetylglucosamine (O-GlcNAc) level decreased. On the basis of NAT10 KD transcriptome and OGA KD transcriptome data, NAT10-mediated ac4C modification of OGA might play a role through G protein–coupled receptors, molecular transduction, nucleosome DNA binding, and other mechanisms in oocyte maturation. Rsph6a, Gm7788, Gm41780, Trpc7, Gm29036, and Gm47144 were potential downstream genes. In conclusion, NAT10 maintained the stability of OGA transcript by ac4C modification on it, thus positively regulating IVM. Moreover, our study revealed the regulation mechanisms of oocytes maturation and provided reference for improving IVM outcomes. At the same time, the interaction between mRNA ac4C modification and protein O-GlcNAc modification was found for the first time, which enriched the regulation network of oocyte maturation.
NLRP3 downregulation enhances engraftment and functionality of adipose-derived stem cells to alleviate erectile dysfunction in diabetic rats
Authors: Luo Chao , Peng Yaqian , Zhou Xiongcai , Fan Junhong , Chen Weihong , Zhang Haibo , Wei Anyang
Published: July 2022

Background

The transplantation of adipose-derived stem cells (ASCs) is a most promising treatment for diabetic erectile dysfunction (DMED). However, the effect of high glucose on the post-transplantation survival of stem cells limits the efficacy of ASCs transplantation. Prolonging the survival time of ASCs in vivo after transplantation is a key issue in the utilization of ASCs for DMED. Herein, we aimed to investigate the therapeutic effect of ASCs by downregulating NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) as well as its mechanism of action in DMED.

Methods

ASCs were obtained by isolating subcutaneous fat from SD rats and were identified using lipogenic and osteogenic differentiation assays, as well as flow cytometric analysis. The shNLRP3 lentivirus with the best downregulating effect was screened, and shNLRP3 lentivirus (LV-shNLRP3) was transfected into ASCs (ASCsshNLRP3) to detect apoptosis and the reactive oxygen species (ROS) levels in each group under high glucose conditions. In DMED rats, ASCsLV-shNLRP3, ASCsLV-control, or phosphate buffered saline (PBS) were administrated via intra-cavernous injection, and normal rats served as normal controls. One week post-injection, animal imaging was performed to track the ASCs. Four weeks post-injection, erectile function was evaluated by measuring the intra-cavernosal pressure and mean arterial pressure. Corpus cavernosum pyroptosis and endothelial function were examined by western blotting and immunofluorescence.

Results

NLRP3-mediated pyroptosis might be a pathogenic mechanism of ED and DMED. ASCs were isolated successfully. Thereafter, the LV-shNLRP3 with the highest transfection efficiency was selected and used to modify ASCs successfully. LV-shNLRP3 could protect ASCs paracrine function under hyperglycemia through anti-apoptosis and anti-ROS deposition mechanisms. Furthermore, ASCsLV-shNLRP3 showed an advantage in the suppression of pyroptosis compared to ASCsLV-control. The ASCsLV-shNLRP3 group had improved cavernous endothelial function and smooth muscle injury, thus reversing erectile function, and was superior to the ASCsLV-control group.

Conclusions

NLRP3 Inflammasome-mediated pyroptosis might be involved in DMED formation. Intra-cavernous injection of ASCsLV-shNLRP3 could suppress cavernosal pyroptosis, contributing to improved erectile function in DMED rats.
The Different Effects of Skeletal Muscle and Fat Mass on Height Increment in Children and Adolescents Aged 6–11 Years: A Cohort Study From China
Authors: Wu Dingting , Shi Liuhong , Xu Qiongying , Zeng Yuanyuan , Lin Xihua , Li Xiaolin , Zhao Hanxin , Zhu Zhihong , Fu Yeliu , Li Hong , Dong Xuehong
Published: July 2022

Objective

This study aimed to investigate the contribution of body composition including skeletal muscle mass (SMM) and body fat mass (BFM) to longitudinal growth among children and adolescents aged 6–11 years old.

Methods

This cohort study was conducted from the annual health examination between 2019 and 2020. Annual height gain and weight gain and changes in SMM and BFM were calculated and compared between sexes, different nutritional status, and growth curve shifting mode. Spearman analyses and multiple linear regression analysis were performed to identify the impact of SMM, BFM, or body mass index (BMI) on height gain.

Results

Of the 584 subjects, the annual height gains of boys (4.76 cm in the 6–9-year group and 4.63 cm in the 10–11-year group) were significantly lower than those of girls (5.48 and 5.74 cm, respectively). Spearman analysis showed that SMM gain and height gain were positively and significantly correlated in each examination of all children (r = 0.535 for boys and 0.734 for girls, p < 0.001). Conversely, BFM and height gains were negatively (r = -0.5240 for boys and -0.232 for girls, p < 0.001) correlated. Multiple linear regression analysis identified SMM gain as an independent predictor (95% CI: 1.20,1.44) of height gain after adjusting for age, gender, BMI, BFM gain, and percentage of body fat (PBF).

Conclusion

SMM gains, rather than BFM gains, were associated with height gains in children and adolescents aged 6–11 years. Monitoring SMM changes in routine healthcare might motivate children and adolescents to achieve dietary and exercise recommendations, thereby growing taller without gaining excessive weight.