bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: In Situ Expression of a Proliferation-Inducing Ligand in Neuromyelitis Optica
Authors: Baert Laurie , Marignier Romain , Lassmann Hans P. , Huard Bertrand
Published: August 2021

A proliferation inducing ligand (APRIL) mediates a key role in the generation and survival of antibody-inducing plasmocytes. Based on this, APRIL has been targeted in autoimmune diseases including multiple sclerosis (MS) and optic neuritis (ON). In MS lesions, APRIL has a new cellular target, the reactive astrocyte and mediates an immunosuppressive activity. Here, we analyzed APRIL expression in a case of neuromyelitis optica (NMO), another autoimmune neurodegenerative disease, showing selective aquaporin-4 depletion in the spinal cord, complement deposition and infiltration of polymorphonuclear cells. We analyzed by immunohistochemistry the presence of APRIL-producing cells, plasmocytes, astrocytes and the localization of secreted APRIL in a lesion from NMO. Plasmocytes were present close to APRIL-producing cells in meninges. However, our main observation was that APRIL targets reactive astrocytes in this lesion of NMO similarly to MS.
Brain Vital Signs in Elite Ice Hockey: Towards Characterizing Objective and Specific Neurophysiological Reference Values for Concussion Management
Authors: Carrick Frederick R. , Pagnacco Guido , Azzolino Sergio F. , Hunfalvay Melissa , Oggero Elena , Frizzell Tory , Smith Christopher J. , Pawlowski Gabriela , Campbell Natasha K. J. , Fickling Shaun D. , Lakhani Bimal , D’Arcy Ryan C. N.
Published: August 2021

Background:Prior concussion studies have shown that objective neurophysiological measures are sensitive to detecting concussive and subconcussive impairments in youth ice-hockey. These studies monitored brain vital signs at rink-side using a within-subjects design to demonstrate significant changes from pre-season baseline scans. However, practical clinical implementation must overcome inherent challenges related to any dependence on a baseline. This requires establishing the start of normative reference data sets.

Methods:The current study collected specific reference data forN= 58 elite, youth, male ice-hockey players and compared these with a general reference dataset fromN= 135 of males and females across the lifespan. The elite hockey players were recruited to a select training camp through CAA Hockey, a management agency for players drafted to leagues such as the National Hockey League (NHL). The statistical analysis included a test-retest comparison to establish reliability, and a multivariate analysis of covariance to evaluate differences in brain vital signs between groups with age as a covariate.

Findings:Test-retest assessments for brain vital signs evoked potentials showed moderate-to-good reliability (Cronbach’s Alpha > 0.7, Intraclass correlation coefficient > 0.5) in five out of six measures. The multivariate analysis of covariance showed no overall effect for group (p= 0.105), and a significant effect of age as a covariate was observed (p< 0.001). Adjusting for the effect of age, a significant difference was observed in the measure of N100 latency (p= 0.022) between elite hockey players and the heterogeneous control group.

Interpretation:The findings support the concept that normative physiological data can be used in brain vital signs evaluation in athletes, and should additionally be stratified for age, skill level, and experience. These can be combined with general norms and/or individual baseline assessments where appropriate and/or possible. The current results allow for brain vital sign evaluation independent of baseline assessment, therefore enabling objective neurophysiological evaluation of concussion management and cognitive performance optimization in ice-hockey.
Abnormal Functional Connectivity Between the Left Medial Superior Frontal Gyrus and Amygdala Underlying Abnormal Emotion and Premature Ejaculation: A Resting State fMRI Study
Authors: Xu Yan , Zhang Xing , Xiang Ziliang , Wang Qing , Huang Xinfei , Liu Tao , Yang Zhaoxu , Chen Yun , Xue Jianguo , Chen Jianhuai , Yang Jie
Published: August 2021

Introduction

Premature ejaculation (PE) is a common sexual dysfunction and is found to be associated with abnormal emotion. The amygdala plays an important role in the processing of emotion. The process of ejaculation is found to be mediated by the frontal-limbic neural circuits. However, the correlations between PE and emotion are still unclear.

Methods

Resting-state functional magnetic resonance imaging (rs-fMRI) data were acquired in 27 PE patients with stable emotion (SPE), 27 PE patients with abnormal emotion (NPE), and 30 healthy controls (HC). We used rs-fMRI to explore the underlying neural mechanisms in SPE, NPE, and HC by measuring the functional connectivity (FC). Differences of FC values among the three groups were compared when choosing bilateral amygdala as the regions of interest (ROIs). We also explored the correlations between the brain regions showing altered FC values and scores of the premature ejaculation diagnostic tool (PEDT)/Eysenck Personality Inventory about neuroticism (EPQ-N) in the PE group.

Results

When the left amygdala was chosen as the ROI, the SPE group exhibited an increased FC between the left medial superior frontal gyrus (SFGmed) and amygdala compared with the NPE or HC group. When the right amygdala was chosen as the ROI, the NPE group exhibited a decreased FC between the left SFGmed and right amygdala compared with the HC group. In addition, FC values of the left SFGmed had positive correlations with PEDT and negative correlations with EPQ-N scores in the PE group. Moreover, FC values of the left superior temporal gyrus had positive correlations with EPQ-N scores in the PE group.

Conclusion

The increased FC values between the left SFGmed and amygdala could reflect a compensatory cortical control mechanism with the effect of stabilized emotion in the limbic regions of PE patients. Abnormal FC between these brain regions could play a critical role in the physiopathology of PE and could help us in dividing PE into more subtypes.
Association Between Preoperative Factors and In-hospital Mortality in Neonates After Cardiac Surgery in China
Authors: Hu Renjie , Zhu Hongbin , Qiu Lisheng , Hong Haifa , Xu Zhiwei , Zhang Haibo , Chen Hao
Published: August 2021

Background: Little is known about preoperative factors affecting cardiac surgery outcomes of neonates in China. We sought to examine the association between characteristics of neonates with congenital heart disease (CHD) and early postoperative outcomes after cardiac repair in a tertiary care paediatric hospital.

Methods: A single-centre retrospective cohort study of neonates who underwent cardiac surgery between January 2006 and December 2019 was performed. Demographic, institutional, and surgical characteristics of neonates were examined and their association with in-hospital mortality was analysed using multivariable logistic regression models.

Results: During the study period, we analysed the outcomes of 1,078 neonates. In-hospital mortality decreased to 13.8% in the era 2017–2019. The overall in-hospital mortality rate was 16.3%. Normal weight at surgery [odds ratio (OR), 0.63; 95% confidence interval (CI), 0.47–0.85; P = 0.003] was associated with lower mortality risk. Poor health status (emergent: OR, 3.11; 95% CI, 1.96–4.94; P < 0.001; elective: OR, 1.63; 95% CI, 1.11–2.40; P = 0.013), higher Society of Thoracic Surgeons-European Association for Cardio-Thoracic Surgery (STAT) categories (STAT 5 category: OR, 2.58; 95% CI, 1.04–6.43; P = 0.042), and limited individual surgeon experience (surgeon with 5–10 operations per year: OR, 1.43; 95% CI, 1.06–1.95; P = 0.021) were associated with higher odds of early death.

Conclusion: In-hospital mortality after neonatal cardiac surgery remained high in our centre over the past 10 years. Some preoperative aspects, including low-weight at surgery, poor health status, increased surgical complexity, and limited surgeon experience were significantly associated with higher mortality. Based on the observed associations, the necessary practises to be modified, especially in preoperative care, should be identified and assessed in future research.
A Novel Thoracic Ultrasound Measurement After Congenital Diaphragmatic Hernia Repair Identifies Decreased Diaphragmatic Excursion Associated With Adverse Respiratory and Surgical Outcomes
Authors: Ross James T. , Liang Norah E. , Phelps Andrew S. , Squillaro Anthony I. , Vu Lan T.
Published: August 2021

Background and Aim: Congenital diaphragmatic hernia (CDH) is a rare defect often associated with pulmonary hypoplasia and abnormal pulmonary vascular development. Even after successful hernia repair, pulmonary disease may persist into adulthood. Impaired diaphragmatic motility may lead to compromised respiratory function long after index repair. This study investigates whether a novel ultrasound measurement, the diaphragmatic excursion ratio, can be a simple and non-invasive method to evaluate routine diaphragmatic motion after CDH repair, and whether it correlates with adverse surgical and respiratory outcomes.

Materials and Methods: A cross-sectional study was conducted in consecutive patients who presented at medium-term follow-up visit between December 2017 and December 2018 after CDH repair at single pediatric hospital. Transthoracic ultrasound was performed with craniocaudal diaphragmatic excursion measured bilaterally during routine breathing. Diaphragmatic excursion ratios (diaphragmatic excursion of repaired vs. unrepaired side) were calculated and retrospectively compared with clinical data including demographics, length of stay, respiratory adjuncts, oral feeding, and need for gastrostomy.

Results: Thirty-eight patients (median age at ultrasound, 24 months, interquartile range 11–60) were evaluated. Nine patients underwent primary repair, 29 had non-primary repair (internal oblique muscle flap or mesh patch). Patients with a diaphragmatic excursion ratio below the median (0.54) had longer hospital stays (median 77 vs. 28 days, p = 0.0007) more ventilator days (median 16 vs. 9 days, p =0.004), and were more likely to have been discharged on oxygen (68 vs. 16%, p = 0.001). They were also less likely to be exclusively taking oral feeds at 1-year post-surgery (37 vs. 74%, p = 0.02) and more likely to require a gastrostomy tube in the first year of life (74 vs. 21%, p = 0.003).

Conclusions: Transthoracic ultrasound after CDH repair is practical method to assess diaphragm motion, and decreased diaphragm excursion ratio is associated with worse respiratory outcomes, a longer length of stay, and dependence on gastrostomy tube feeding within 1 year. Further prospective studies may help validate this novel ultrasound measurement and offer prognostic value.
Protective Property of Scutellarin Against Liver Injury Induced by Carbon Tetrachloride in Mice
Authors: Miao Zhimin , Lai Yong , Zhao Yingying , Chen Lingmin , Zhou Jianeng , Li Chunyan , Wang Yan
Published: August 2021

Liver injury is a clinical disorder caused by toxins, drugs, and alcohol stimulation without effective therapeutic approaches thus far. Scutellarin (SCU), isolated from the edible herb Erigeron breviscapus (Vant.) Hand. -Mazz. showed potential hepatoprotective effects, but the mechanisms remain unknown. In this study, transcriptomics combined with nontargeted metabolomics and 16S rRNA amplicon sequencing were performed to elucidate the functional mechanisms of SCU in carbon tetrachloride (CCl4)–induced liver injury in mice. The results showed that SCU exerted potential hepatoprotective effects against CCl4-induced liver injury by repressing CYP2E1 and IκBα/NF-κB signaling pathways, modulating the gut microbiota (especially enriching Lactobacillus), and regulating the endogenous metabolites involved in lipid metabolism and bile acid homeostasis. SCU originates from a functional food that appears to be a promising agent to guard against liver injury.
The Relationships Between Hyperprolactinemia, Metabolic Disturbance, and Sexual Dysfunction in Patients With Schizophrenia Under Olanzapine Treatment
Authors: Wu Tzu-Hua , Lin Chieh-Hsin , Goh Kah Kheng , Chen Cynthia Yi-An , Chen Chun-Hsin , Lane Hsien-Yuan , Lu Mong-Liang
Published: August 2021

The aim of the study was to assess the relationship between prolactin levels and sexual dysfunction in patients with schizophrenia who use olanzapine medication. The potential risk factors of hyperprolactinemia and sexual dysfunction were also investigated. Patients with schizophrenia undergoing olanzapine monotherapy were invited to participate in this cross-sectional study. The Arizona Sexual Experiences Scale (ASEX) and the Positive and Negative Syndrome Scale were used to evaluate subjective sexual dysfunction and psychopathology, respectively. Levels of prolactin and metabolic parameters were also measured. In total, 279 participants with schizophrenia were recruited. The overall incidences of hyperprolactinemia, sexual dysfunction, and metabolic syndrome were 51.6, 53.8, and 43.7%, respectively. Higher ASEX scores, higher insulin levels, female sex, and younger age were associated with hyperprolactinemia. Prolactin level was significantly correlated with ASEX score. Elevated prolactin levels, concomitant antidepressant, increased insulin resistance, longer illness duration, and female sex were associated with sexual dysfunction. Female participants recorded higher levels of sexual dysfunction than their male counterparts did, whereas male participants had comparatively lower prolactin levels and lower rates of spousal partnership. Hyperprolactinemia, metabolic syndrome, and sexual dysfunction are prevalent in patients with schizophrenia treated with olanzapine. Clinicians should maintain awareness of these problems and monitor them regularly with their patients.
Molecular Mechanisms Underlying the Acclimation of Chlamydomonas reinhardtii Against Nitric Oxide Stress
Authors: Kuo Eva YuHua , Lee Tse-Min
Published: August 2021

The acclimation mechanism ofChlamydomonas reinhardtiito nitric oxide (NO) was studied by exposure toS-nitroso-N-acetylpenicillamine (SNAP), a NO donor. Treatment with 0.1 or 0.3 mM SNAP transiently inhibited photosynthesis within 1 h, followed by a recovery, while 1.0 mM SNAP treatment caused irreversible photosynthesis inhibition and mortality. The SNAP effects are avoided in the presence of the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-l-oxyl-3-oxide (cPTIO). RNA-seq, qPCR, and biochemical analyses were conducted to decode the metabolic shifts under NO stress by exposure to 0.3 mM SNAP in the presence or absence of 0.4 mM cPTIO. These findings revealed that the acclimation to NO stress comprises a temporally orchestrated implementation of metabolic processes: (1). modulation of NADPH oxidase (respiratory burst oxidase-like 2, RBOL2) and ROS signaling pathways for downstream mechanism regulation, (2). trigger of NO scavenging elements to reduce NO level; (3). prevention of photo-oxidative risk through photosynthesis inhibition and antioxidant defense system induction; (4). acclimation to nitrogen and sulfur shortage; (5). attenuation of transcriptional and translational activity together with degradation of damaged proteins through protein trafficking machinery (ubiquitin, SNARE, and autophagy) and molecular chaperone system for dynamic regulation of protein homeostasis. In addition, the expression of the gene encoding NADPH oxidase, RBOL2, showed a transient increase while that of RBOL1 was slightly decreased after NO challenge. It reflects that NADPH oxidase, a regulator in ROS-mediated signaling pathway, may be involved in the responses ofChlamydomonasto NO stress. In conclusion, our findings provide insight into the molecular events underlying acclimation mechanisms inChlamydomonasto NO stress.
Physiological Response of Cape Gooseberry Plants to Fusarium oxysporum f. sp. physali, Fusaric Acid, and Water Deficit in a Hydrophonic System
Authors: Mendoza-Vargas Luis Alberto , Villamarín-Romero Wendy Paola , Cotrino-Tierradentro Anderson Steven , Ramírez-Gil Joaquín Guillermo , Chávez-Arias Cristhian Camilo , Restrepo-Díaz Hermann , Gómez-Caro Sandra
Published: August 2021

Cape gooseberry production has been limited by vascular wilt caused by Fusarium oxysporum f. sp. physali (Foph). Fusaric acid (FA) is a mycotoxin produced by many Fusarium species such as F. oxysporum formae speciales. The effects of the interaction between this mycotoxin and plants (such as cape gooseberry) under biotic stress (water deficit, WD) have been little explored. Three experiments were carried out. The objectives of this study were to evaluate (i) different Foph inoculum densities (1 × 104 and 1 × 106 conidia ml−1; experiment (1); (ii) the effect of times of exposure (0, 6, 9, and 12 h) and FA concentrations (0, 12.5, 25, 50, and 100 mg L−1; experiment (2), and (iii) the interaction between Foph (1 × 104 conidia mL−1) or FA (25 mg L−1 × 9 h), and WD conditions (experiment 3) on the physiological (plant growth, leaf stomatal conductance (gs), and photochemical efficiency of PSII (Fv/Fm ratio) and biochemical [malondialdehyde (MDA) and proline] responses of cape gooseberry seedling ecotype Colombia. The first experiment showed that Foph inoculum density of 1 × 106 conidia ml−1 caused the highest incidence of the disease (100%). In the second experiment, gs (~40.6 mmol m−2 s−1) and Fv/Fm ratio (~0.59) decreased, whereas MDA (~9.8 μmol g−1 FW) increased in plants with exposure times of 9 and 12 h and an FA concentration of 100 mg L−1 compared with plants without FA exposure or concentrations (169.8 mmol m−2 s−1, 0.8, and 7.2 μmol g−1 FW for gs, Fv/Fm ratio and MDA, respectively). In the last experiment, the interaction between Foph or FA and WD promoted a higher area under the disease progress curve (AUDPC) (Foph × WD = 44.5 and FA × WD = 37) and lower gs (Foph × WD = 6.2 mmol m−2 s−1 and FA × WD = 9.5 mmol m−2 s−1) compared with plants without any interaction. This research could be considered as a new approach for the rapid scanning of responses to the effects of FA, Foph, and WD stress not only on cape gooseberry plants but also on other species from the Solanaceae family.
Does Shoot Apical Meristem Function as the Germline in Safeguarding Against Excess of Mutations?
Authors: Burian Agata
Published: August 2021

A genetic continuity of living organisms relies on the germline which is a specialized cell lineage producing gametes. Essential in the germline functioning is the protection of genetic information that is subjected to spontaneous mutations. Due to indeterminate growth, late specification of the germline, and unique longevity, plants are expected to accumulate somatic mutations during their lifetime that leads to decrease in individual and population fitness. However, protective mechanisms, similar to those in animals, exist in plant shoot apical meristem (SAM) allowing plants to reduce the accumulation and transmission of mutations. This review describes cellular- and tissue-level mechanisms related to spatio-temporal distribution of cell divisions, organization of stem cell lineages, and cell fate specification to argue that the SAM functions analogous to animal germline.
Overexpression of OsHMGB707, a High Mobility Group Protein, Enhances Rice Drought Tolerance by Promoting Stress-Related Gene Expression
Authors: Xu Kai , Chen Shoujun , Li Tianfei , Yu Shunwu , Zhao Hui , Liu Hongyan , Luo Lijun
Published: August 2021

Drought stress adversely affects crop growth and productivity worldwide. In response, plants have evolved several strategies in which numerous genes are induced to counter stress. High mobility group (HMG) proteins are the second most abundant family of chromosomal proteins. They play a crucial role in gene transcriptional regulation by modulating the chromatin/DNA structure. In this study, we isolated a novel HMG gene, OsHMGB707, one of the candidate genes localized in the quantitative trait loci (QTL) interval of rice drought tolerance, and examined its function on rice stress tolerance. The expression of OsHMGB707 was up-regulated by dehydration and high salt treatment. Its overexpression significantly enhanced drought tolerance in transgenic rice plants, whereas its knockdown through RNA interference (RNAi) did not affect the drought tolerance of the transgenic rice plants. Notably, OsHMGB707-GFP is localized in the cell nucleus, and OsHMGB707 is protein-bound to the synthetic four-way junction DNA. Several genes were up-regulated in OsHMGB707-overexpression (OE) rice lines compared to the wild-type rice varieties. Some of the genes encode stress-related proteins (e.g., DREB transcription factors, heat shock protein 20, and heat shock protein DnaJ). In summary, OsHMGB707 encodes a stress-responsive high mobility group protein and regulates rice drought tolerance by promoting the expression of stress-related genes.
Homo- and Dikaryons of the Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Differ in Life History Strategy
Authors: Serghi Edward Umberto , Kokkoris Vasilis , Cornell Calvin , Dettman Jeremy , Stefani Franck , Corradi Nicolas
Published: August 2021

Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts that have the potential to improve crop yield. These multinucleate organisms are either “homokaryotic” or “dikaryotic”. In AMF dikaryons, thousands of nuclei originating from two parental strains coexist in the same cytoplasm. In other fungi, homokaryotic and dikaryotic strains show distinct life history traits (LHTs), such as variation in growth rates and fitness. However, how such traits compare between dikaryons and homokaryons of AMF is unknown. To address this, we measured 20 LHT of four dikaryons and five homokaryons of the model fungus Rhizophagus irregularis across root organ cultures of three host plants (carrot, chicory, and tobacco). Our analyses show that dikaryons have clearly distinct life history strategies (LHSs) compared to homokaryons. In particular, spores of homokaryons germinate faster and to a higher proportion than dikaryons, whereas dikaryons grow significantly faster and create a more complex hyphal network irrespective of host plant species. Our study links AMF nuclear status with key LHT with possible implications for mycorrhizal symbiotic functioning.
The Effects of Emotional Design on Multimedia Learning and Appreciation of Chinese Poetry
Authors: Wang Yi , Zhou Zhijin , Gong Shaoying , Jia Dandan , Lei Jing
Published: August 2021

Painting, music, literature, and other art forms embody the essence of human wisdom and induce esthetic experience, among which poetry is inherently creative, because it contains a wealth of symbols, imageries, insights, and so forth. The appreciation and learning of Chinese poetry is an important part of the curriculum in secondary schools. However, studies have mainly focused on textual characters of poetry, with little literature focusing on esthetic appreciation and in-depth learning of poetry. In this vein, we ask whether emotional designs will promote the appreciation and learning of Chinese poetry. To answer this question, we explored the influence of the combination of external emotion induction (positive and neutral movie clips) and internal colorful design (chromatic and achromatic) on esthetic preference and learning of poetry. One hundred and sixty-six participants (14–15 years old) were randomly assigned to one of four conditions created by two factors (external emotion induction and internal colorful design). The results showed that the combination of external emotion induction and internal colorful design promoted positive emotions, retention, and transfer performances of learners. Furthermore, perceived difficulty of learners decreased significantly when external emotional induction and internal colorful design were both positive. Consequently, these findings indicated that emotional designs in multimedia facilitated the learning performance of middle school students in Chinese poetry, and supported the cognitive-affective theory of learning with media. This research was a preliminary exploration of emotional design in humanities.
Depression, Anxiety, and Suicidal Ideation in Chinese University Students During the COVID-19 Pandemic
Authors: Zhou Shuang-Jiang , Wang Lei-Lei , Qi Meng , Yang Xing-Jie , Gao Lan , Zhang Suo-Yuan , Zhang Li-Gang , Yang Rui , Chen Jing-Xu
Published: August 2021

Coronavirus disease-2019 (COVID-19) pandemic has seriously threatened the global public health security and caused a series of mental health problem. Current research focuses mainly on mental health status and related factors in the COVID-19 pandemic among Chinese university students. Data from 11133 participants was obtained through an online survey. The Patient Health Question-9 (PHQ-9) was used to assess depressive symptoms, the Social Support Rate Scale (SSRS) was used to assess social support. We also used 7-item Generalized Anxiety Disorder Scale (GAD-7) to assess anxiety symptoms. Totally, 37.0% of the subjects were experiencing depressive symptoms, 24.9% anxiety symptoms, 20.9% comorbid depressive and anxiety symptoms, and 7.3% suicidal ideation. Multivariable logistic regression analysis revealed an increased presence of mental health problems in female students, graduate students, and those with personal COVID-19 exposure. Awareness of COVID-19, living with family were protective factors that reduced anxiety and depression symptoms. In addition, male, personal COVID-19 exposure, depressive and anxiety symptoms were risk factors for suicidal ideation. Social support, COVID-19 preventive and control measures, prediction of COVID-19 trends, living with family and graduate students are protective factors for reducing suicidal ideation.
I Do It, but I Decide With Whom
Authors: Pulido Cristina M. , Vidu Ana , Racionero-Plaza Sandra , Puigvert Lídia
Published: August 2021

Social interactions and communication shape the desires and preferences of men and women. While it is true that some men have modified their behavior due to feminist women, the same happened with some women, who changed attraction patterns thanks to new alternative masculinities (NAM). This study examines the latter, focusing on social interactions mediated by language, as a crucial element to impact and change the desires of people. For this purpose, six autobiographical interviews were conducted with women aged 19–39 years, from two different countries and continents, paying attention to the narratives of their sexual-affective relationships. Using the communicative methodology, interactions have been analyzed from verbal communication and nonverbal communication, based on the consequences of the actions rather than intentionality. The results of this study show how dialogic communicative acts with NAMs influenced some women who first defended or justified actions of male perpetrators to later prefer to support female survivors against their perpetrators. Analysis reveals that communicative acts grounded in such language that enacted the desire of NAM for women of solidarity have shaped some memories of women of relationships with dominant traditional masculinities (DTM) and, ultimately, contributed to change their attraction and election patterns.
Relationship Between Social Anxiety and Internet Addiction in Chinese College Students Controlling for the Effects of Physical Exercise, Demographic, and Academic Variables
Authors: Ye Songdong , Cheng Huiying , Zhai Zongpeng , Liu Hongyou
Published: August 2021

This study aims to identify the relationship between social anxiety (SA) and internet addiction (IA) in a group of Chinese college students by controlling for the effects of physical exercise (PE), demographic, and academic variables. A sample of 4,677 students from five major regions of China participated in this survey. The findings revealed that: (1) SA had a direct effect on IA; (2) regular and active participation in physical exercise can relieve SA and IA effectively; (3) the level of SA and IA is strongly linked to sex; (4) the levels of SA and IA are different among students of different majors; (5) students in the middle phase of their academic career are more likely to have IA than those in the starting phase. The study is significant because few existing studies discuss the role of PE on SA and IA. Additionally, the study found that college students with more PE would have a lower level of SA and a lower probability of IA.
Water Use Efficiency: A Review of Contextual and Behavioral Factors
Authors: Callejas Moncaleano Diana Carolina , Pande Saket , Rietveld Luuk
Published: August 2021

Water withdrawals around the world have increased almost twice as fast as the population during the last century. Higher than expected water demand is leading to water scarcity and causing rapid depletion of water tables around the world. One reason behind the higher than expected demand is the inefficient use of water. Inefficient use of water affects the well-being of society, the economic stability of countries, and environmental health. Indeed, water use efficiency (WUE) is one of the pillars of sustainable development goals (SDG 6.4.1). However, progress toward achieving WUE is slow, especially for many developing countries where the degradation of natural resources is critical, economic growth is slow, and there are few strong institutions to coordinate actions. One reason behind inefficient water use is human behavior. A variety of contextual and psychological factors underlie the behavior. The contextual factors include socioeconomic, technical, institutional, and environmental factors and the behavioral factors include factors associated with the perception of risk, attitudes, norms, etc. Yet, few studies consider an integrated view of these factors in shaping water use behavior. This paper consolidates contextual and behavioral factors which influence water use, studies the gaps in our understanding of human water behavior underlying WUE and highlights the need to comprehensive assess and consistently measure such factors and their relationships. Based on the gaps identified, it proposes a conceptual model that connects contextual and behavioral factors and represents potential cause-effect relationships as supported by various environmental behavior approaches and psychological theories. Based on the literature review of water use, and conservation behavior, environmental psychology, and water use models, this model proposes an institutional factor to assess the relationship between institutions and stakeholders, and study contextual factors linked not only for individual water users but also studying these factors for individuals of water supply organizations.
Cross-Validation Indicates Predictive Models May Provide an Alternative to Indicator Organism Monitoring for Evaluating Pathogen Presence in Southwestern US Agricultural Water
Authors: Belias Alexandra , Brassill Natalie , Roof Sherry , Rock Channah , Wiedmann Martin , Weller Daniel
Published: August 2021

Pathogen contamination of agricultural water has been identified as a probable cause of recalls and outbreaks. However, variability in pathogen presence and concentration complicates the reliable identification of agricultural water at elevated risk of pathogen presence. In this study, we collected data on the presence of Salmonella and genetic markers for enterohemorrhagic E. coli (EHEC; PCR-based detection of stx and eaeA) in southwestern US canal water, which is used as agricultural water for produce. We developed and assessed the accuracy of models to predict the likelihood of pathogen contamination of southwestern US canal water. Based on 169 samples from 60 surface water canals (each sampled 1–3 times), 36% (60/169) and 21% (36/169) of samples were positive for Salmonella presence and EHEC markers, respectively. Water quality parameters (e.g., generic E. coli level, turbidity), surrounding land-use (e.g., natural cover, cropland cover), weather conditions (e.g., temperature), and sampling site characteristics (e.g., canal type) data were collected as predictor variables. Separate conditional forest models were trained for Salmonella isolation and EHEC marker detection, and cross-validated to assess predictive performance. For Salmonella, turbidity, day of year, generic E. coli level, and % natural cover in a 500–1,000 ft (~150–300 m) buffer around the sampling site were the top 4 predictors identified by the conditional forest model. For EHEC markers, generic E. coli level, day of year, % natural cover in a 250–500 ft (~75–150 m) buffer, and % natural cover in a 500–1,000 ft (~150–300 m) buffer were the top 4 predictors. Predictive performance measures (e.g., area under the curve [AUC]) indicated predictive modeling shows potential as an alternative method for assessing the likelihood of pathogen presence in agricultural water. Secondary conditional forest models with generic E. coli level excluded as a predictor showed < 0.01 difference in AUC as compared to the AUC values for the original models (i.e., with generic E. coli level included as a predictor) for both Salmonella (AUC = 0.84) and EHEC markers (AUC = 0.92). Our data suggests models that do not require the inclusion of microbiological data (e.g., indicator organism) show promise for real-time prediction of pathogen contamination of agricultural water (e.g., in surface water canals).
Targeting Soluble Epoxide Hydrolase and Cyclooxygenases Enhance Joint Pain Control, Stimulate Collagen Synthesis, and Protect Chondrocytes From Cytokine-Induced Apoptosis
Authors: Tucker Laura , Trumble Troy N. , Groschen Donna , Dobbs Erica , Baldo Caroline F. , Wendt-Hornickle Erin , Guedes Alonso G. P.
Published: August 2021

Objective:To determine the symptomatic and disease-modifying capabilities of sEH and COX inhibitors during joint inflammation.

Methods:Using a blinded, randomized, crossover experimental design, 6 adult healthy horses were injected with lipopolysaccharide (LPS; 3 μg) fromE. coliin a radiocarpal joint and concurrently received the non-selective cyclooxygenase (COX) inhibitor phenylbutazone (2 mg/kg), the sEH inhibitort-TUCB (1 mg/kg) or both (2 mg/kg phenylbutazone and 0.1, 0.3, and 1 mg/kgt-TUCB) intravenously. There were at least 30 days washout between treatments. Joint pain (assessedviainertial sensors and peak vertical forces), synovial fluid concentrations of prostanoids (PGE2, TxB2), cytokines (IL-1β, IL-6, TNF-α) and biomarkers of collagen synthesis (CPII) and degradation (C2C) were measured at pre-determined intervals over a 48-h period. The anti-apoptotic effect of COX and sEH inhibitors was determinedviaELISA technique in primary equine chondrocytes incubated with TNF-α (10 ng/ml) for 24 h. Apoptosis was also determined in chondrocytes incubated with sEH-generated metabolites.

Results:Combined COX and sEH inhibition produced significantly better control of joint pain, prostanoid responses, and collagen synthesis-degradation balance compared to each compound separately. When administered separately, pain control was superior with COXvs. sEH inhibition. Cytokine responses were not different during COX and/or sEH inhibition. In cultured chondrocytes, sEH inhibition alone or combined with COX inhibition, but not COX inhibition alone had significant anti-apoptotic effects. However, sEH-generated metabolites caused concentration-dependent apoptosis.

Conclusions:Combined COX and sEH inhibition optimize pain control, attenuate loss of articular cartilage matrix during joint inflammation and cytokine-induced chondrocyte apoptosis.
The Proteome of Equine Oviductal Fluid Varies Before and After Ovulation: A Comparative Study
Authors: Fernández-Hernández Pablo , Marinaro Federica , Sánchez-Calabuig María Jesús , García-Marín Luis Jesús , Bragado María Julia , González-Fernández Lauro , Macías-García Beatriz
Published: August 2021

Equine fertilization cannot be performed in the laboratory as equine spermatozoa do not cross the oocyte's zona pellucida in vitro. Hence, a more profound study of equine oviductal fluid (OF) composition at the pre-ovulatory and post-ovulatory stages could help in understanding what components are required to achieve fertilization in horses. Our work aimed to elucidate the proteomic composition of equine OF at both stages. To do this, OF was obtained postmortem from oviducts of slaughtered mares ipsilateral to a pre-ovulatory follicle (n = 4) or a recent ovulation (n = 4); the samples were kept at −80°C until analysis. After protein extraction and isobaric tags for relative and absolute quantification (iTRAQ) labeling, the samples were analyzed by nano-liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The analysis of the spectra resulted in the identification of a total of 1,173 proteins present in pre-ovulatory and post-ovulatory samples; among these, 691 were unique for Equus caballus. Proteins from post-ovulatory oviductal fluid were compared with the proteins from pre-ovulatory oviductal fluid and were categorized as upregulated (positive log fold change) or downregulated (negative log fold change). Fifteen proteins were found to be downregulated in the post-ovulatory fluid and 156 were upregulated in the post-ovulatory OF compared to the pre-ovulatory fluid; among the upregulated proteins, 87 were included in the metabolism of proteins pathway. The identified proteins were related to sperm–oviduct interaction, fertilization, and metabolism, among others. Our data reveal consistent differences in the proteome of equine OF prior to and after ovulation, helping to increase our understanding in the factors that promote fertilization and early embryo development in horses.
Design and Structural Analyses of a Reciprocating S1223 High-Lift Wing for an RA-Driven VTOL UAV
Authors: Imumbhon Johnson O. , Alam Mohammad D. , Cao Yiding
Published: August 2021

In the design stage of an aircraft, structural analyses are commonly employed to test the integrity of the aircraft components to demonstrate the capability of the structural elements to withstand what they are designed for, as well as predict potential failure of the components. This research focused on the structural design and analysis of a high-lift, low Reynolds number airfoil profile, the Selig S1223, under reciprocating inertial force loading, to determine the feasibility of its use in a new reciprocating airfoil (RA) driven VTOL UAV. The material selected for the wing structures including ribs, spars, and skin, was high-strength carbon fiber. The wing was designed in SolidWorks, while finite element analysis was performed with ANSYS mechanical in conjunction with the inertia forces due to the reciprocating motion of the wing and the lift and drag forces that were derived from the aerodynamic wing analyses. The structural stress and strain determined under the loading conditions were satisfactory and the designed wing could sustain the high reciprocating inertia forces in the RA-driven VTOL UAV module. The results of this study indicate that the Selig S1223 airfoil profile, due to its superior performance at low Reynolds numbers, high-lift, and reduced noise characteristics at low angles of attack, combined with the use of the high strength carbon fiber, proves to be an excellent choice for this RA-driven aircraft application.
Chemical Features and Bioactivities of Lactuca canadensis L., an Unconventional Food Plant from Brazilian Cerrado
Authors: Liberal Ângela , Coelho Carla T. P. , Fernandes Ângela , Cardoso Rossana V. C. , Dias Maria Inês , Pinela José , Alves Maria José , Severino Vanessa G. P. , Ferreira Isabel C. F. R. , Barros Lillian
Published: August 2021

Throughout the world, people’s diet is generally quite restricted regarding the variety of plants used in their daily regime. The Unconventional Food Plant (UFP) Lactuca canadensis L. is an edible species of wild lettuce sparsely described in literature and considered to be native from the eastern and central parts of North America. To valorize this species as potential alternative food, an analysis of its nutritional, chemical, and bioactive properties was performed. The results specify the occurrence of organic acids, mainly quinic acid (127.9 ± 0.6 g/kg dry weight (dw)), polyunsaturated fatty acids (65.3%), among which are linolenic acid (44.4 ± 0.4 %), and tocopherols, mostly α-tocopherol (61.2 ± 0.7 mg/kg dw). Additionally, eight phenolic compounds were also identified, among which luteolin-O-glucuronide was found in larger amounts in both infusion and hydroethanolic extracts (5.46 ± 0.09 and 4.6 ± 0.1 mg/g dw, respectively). Carbohydrates and proteins were the main macronutrients (603 ± 1 and 177.5 ± 0.3 g/kg dw, respectively), followed by ashes (166.5 ± 0.9), indicative of a great amount of minerals. Additionally, good antioxidant and antibacterial activities were detected in the analyzed extracts. In general, our results contribute to extend the range of different, unexploited, and nutritionally balanced plant foods, such as Lactuca canadensis, that can and should be included in the daily diet.
The Combined Effect of Weaning Stress and Immune Activation during Pig Gestation on Serum Cytokine and Analyte Concentrations
Authors: Rymut Haley E. , Rund Laurie A. , Bolt Courtni R. , Villamil Maria B. , Southey Bruce R. , Johnson Rodney W. , Rodriguez-Zas Sandra L.
Published: August 2021

Weaning stress can elicit changes in the metabolic, hormone and immune systems of pigs and interact with prolonged disruptions stemming from maternal immune activation (MIA) during gestation. The present study advances the characterization of the combined effects of weaning stress and MIA on blood chemistry, immune and hormone indicators that inform on the health of pigs. Three-week-old female and male offspring of control gilts or gilts infected with the porcine reproductive and respiratory syndrome virus were allocated to weaned or nursed groups. The anion gap and bilirubin profiles suggest that MIA enhances tolerance to the effects of weaning stress. Interleukin 1 beta and interleukin 2 were highest among weaned MIA females, and cortisol was higher among weaned relative to nursed pigs across sexes. Canonical discriminant analysis demonstrated that weaned and nursed pigs have distinct chemistry profiles, whereas MIA and control pigs have distinct cytokine profiles. The results from this study can guide management practices that recognize the effects of the interaction between MIA and weaning stress on the performance and health of pigs.
Immunohistochemical Characterization of Tumor-Associated Macrophages in Canine Lymphomas
Authors: Vázquez Sergio , Vallejo Raquel , Espinosa José , Arteche Noive , Vega José A. , Pérez Valentín
Published: August 2021

Macrophages have been confirmed to play a significant role in the behavior of human lymphomas, albeit no consistent data are so far available in canine lymphomas. The present study characterizes the macrophages present in cases of canine nodal lymphoma and their relationship with the histological grade and the immunophenotype. Samples from the lymph nodes of 25 dogs diagnosed with lymphoma were selected. Immunohistochemistry was used to determine the tumor immunophenotype (CD3 and CD20 antibodies) and macrophage characterization (Iba1, MAC387, CD204, CD163 and iNOS antibodies). Macrophage counting was performed in 10 randomly selected, high-power fields per sample. Generalized linear models with Poisson distribution were used for statistical analysis. A significantly greater number of macrophages (Iba1+) were detected in high-grade and B-cell lymphomas. The highest amount of both M1 (iNOS+) and M2 (CD204+ and CD163+) subtypes were observed in B-cell lymphomas. High-grade lymphomas showed a greater number of CD204+ and CD163+ cells and recently recruited MAC387+ macrophages. The latter were most abundant in T than in B-cell lymphomas. In conclusion, a significant population of macrophages is present in canine lymphomas, which constitute a heterogeneous population that shows variations in the amount and immunohistochemical profile according to the histological grade and immunophenotype.
Microbiome-Metabolites Analysis Reveals Unhealthy Alterations in the Gut Microbiota but Improved Meat Quality with a High-Rice Diet Challenge in a Small Ruminant Model
Authors: Wang Kaijun , Peng Xiaomin , Lv Feifei , Zheng Mengli , Long Donglei , Mao Hongxiang , Si Hongbin , Zhang Peihua
Published: August 2021

Effects of a high-rice dietary proportion on the meat quality, acute phase reaction proteins (APRPs) and colonic microbiota and metabolites in goats are rarely reported. This study was designed to investigate the meat quality and metabolism in goats. Sixteen goats were equally divided into two groups and fed a control diet (Con, 55% concentrate) or a high-rice diet (HR, 90% concentrate) for five weeks. We found that the HR diet improved the slaughtering characteristic and meat quality but induced an acute phase reaction and decreased bacterial richness and diversity when compared to the control group. Furthermore, the levels of acetate, propionate and total VFA concentrations were higher in the colonic contents of the HR-fed goats than in those of the control group (p < 0.05). Meanwhile, the HR diet decreased the pH value, lactic acid concentration and increased the activity of amylase and lipopolysaccharide concentration in the colonic contents of goats (p < 0.05). The proportion of Oscillibacter increased while Phocaeicola and Christensenellaceae_R-7_group significantly decreased with the HR diet (p < 0.05). Collectively, the HR diet induced an acute phase reaction and altered the colonic bacterial community, which increases the health risk to growing goats.
Effect of GP19 Peptide Hyperimmune Antiserum on Activated Macrophage during Ehrlichia canis Infection in Canine Macrophage-like Cells
Authors: Nambooppha Boondarika , Rittipornlertrak Amarin , Muenthaisong Anucha , Koonyosying Pongpisid , Tangtrongsup Sahatchai , Tiwananthagorn Saruda , Chung Yang-Tsung , Sthitmatee Nattawooti
Published: August 2021

In terms of its veterinary importance, vaccine development against Ehrlichia canis is needed. However, the effect of developing vaccines on humoral immune response against E. canis infection is still unknown. Novel GP194-43 was synthesized according to E. canis GP19 epitope prediction. To restrict any loss and/or illness in the host animal, rabbits were used in this study to produce GP194-43 hyperimmune sera. The effect of GP194-43 hyperimmune sera on neutralization was examined in vitro by determining the inhibition of E. canis infection of the macrophage-like cell line (DH82) in the presence of the sera. Four groups of DH82 cells received differing treatments. These included E. canis experimentally infected DH82 cells, E. canis-infected DH82 cells with control rabbit serum (untreated group), E. canis-infected DH82 cells with GP194-43 rabbit antiserum (treated group) and uninfected cells (negative control group), respectively. The treated group developed a decrease (p < 0.01) in the percentage of E. canis infected cells after 3 days post-infection at 48.57 ± 1.28. In addition, real-time PCR analyses of cytokine mRNA expression involved with the macrophage, humoral, and cellular immune responses were conducted. The findings revealed an upregulated expression of IFNG in the treated group during the infection. This study demonstrated neutralization in the GP194-43 peptide hyperimmune sera of immunized rabbits. Notably, IFN-γ production could be effectively promoted in canine macrophages in relation to the activation of macrophages and adaptive immune responses. The results of this study indicate the potential for the use of this immunogen in further investigations involving immunized and infected dogs as E. canis host species.