bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular mechanisms of anthracycline induced cardiotoxicity: Zebrafish come into play
Authors: Moossavi Maryam , Lu Xiaoguang , Herrmann Joerg , Xu Xiaolei
Published: March 2023

Anthracyclines are among the most potent chemotherapeutics; however, cardiotoxicity significantly restricts their use. Indeed, anthracycline-induced cardiotoxicity (AIC) fares among the worst types of cardiomyopathy, and may only slowly and partially respond to standard heart failure therapies including β-blockers and ACE inhibitors. No therapy specifically designed to treat anthracycline cardiomyopathy at present, and neither is it known if any such strategy could be developed. To address this gap and to elucidate the molecular basis of AIC with a therapeutic goal in mind, zebrafish has been introduced as an in vivo vertebrate model about a decade ago. Here, we first review our current understanding of the basic molecular and biochemical mechanisms of AIC, and then the contribution of zebrafish to the AIC field. We summarize the generation of embryonic zebrafish AIC models (eAIC) and their use for chemical screening and assessment of genetic modifiers, and then the generation of adult zebrafish AIC models (aAIC) and their use for discovering genetic modifiers via forward mutagenesis screening, deciphering spatial-temporal-specific mechanisms of modifier genes, and prioritizing therapeutic compounds via chemical genetic tools. Several therapeutic target genes and related therapies have emerged, including a retinoic acid (RA)-based therapy for the early phase of AIC and an autophagy-based therapy that, for the first time, is able to reverse cardiac dysfunction in the late phase of AIC. We conclude that zebrafish is becoming an important in vivo model that would accelerate both mechanistic studies and therapeutic development of AIC.
Twenty-five year experience with aortic valve-sparing root replacement in a single teaching center
Authors: Sromicki Juri , Van Hemelrijck Mathias , Schmiady Martin O. , Greutmann Matthias , Bonassin Tempesta Francesca , Mestres Carlos-A. , Vogt Paul R. , Carrel Thierry P. , Holubec Tomáš
Published: March 2023

Objectives

Aortic valve-sparing root replacement (AVSRR) is a technically demanding procedure. In experienced centers it offers excellent short- and long-term results, making the procedure an attractive alternative for aortic root replacement especially in young patients. The aim of this study was to analyze long-term results of AVSRR using the David operation in our institution over the last 25 years.

Methods

This is a single-center retrospective analysis of outcomes of David operations performed in a teaching institution not running a large AVSRR-program. Pre-, intra- and postoperative data were collected from the institutional electronic medical record system. Follow-up data were collected through direct contact of the patients and their cardiologists/primary care physicians.

Results

Between 02/1996 and 11/2019, 131 patients underwent David operation in our institution by a total of 17 different surgeons. Median age was 48 (33–59), 18% were female. Elective surgery was performed in 89% of the cases, 11% were operated as emergency in the setting of an acute aortic dissection. Connective tissue disease was present in 24% and 26% had a bicuspid aortic valve. At hospital admission 61% had aortic regurgitation grade ≥3, 12% were in functional NYHA-class ≥III. 30-day mortality was 2%, 97% of the patients were discharged with aortic regurgitation ≤2. In 10-year follow-up, 15 (12%) patients had to be re-operated because of root-related complications. Seven patients (47%) received a transcatheter aortic valve implantation, 8 (53%) required surgical replacement of the aortic valve or a Bentall-De Bono operation. Estimated reoperation-free survival at 5 and 10 years was 93.5% ± 2.4% and 87.0% ± 3.5%, respectively. Subgroup analysis showed no differences in reoperation-free survival for patients presenting with a bicuspid valve or preoperative aortic regurgitation ≥3. However a preoperative left ventricular end diastolic diameter of ≥5.5 cm was associated with worse outcome.

Conclusion

David operations can be performed with excellent perioperative and 10-year follow-up outcomes in centers not running large AVSRR-programs.
Effect of PCSK9 on atherosclerotic cardiovascular diseases and its mechanisms: Focus on immune regulation
Authors: Ma Minglu , Hou Chang , Liu Jian
Published: March 2023

Atherosclerosis is a basic pathological characteristic of many cardiovascular diseases, and if not effectively treated, patients with such disease may progress to atherosclerotic cardiovascular diseases (ASCVDs) and even heart failure. The level of plasma proprotein convertase subtilisin/kexin type 9 (PCSK9) is significantly higher in patients with ASCVDs than in the healthy population, suggesting that it may be a promising new target for the treatment of ASCVDs. PCSK9 produced by the liver and released into circulation inhibits the clearance of plasma low-density lipoprotein-cholesterol (LDL-C), mainly by downregulating the level of LDL-C receptor (LDLR) on the surface of hepatocytes, leading to upregulated LDL-C in plasma. Numerous studies have revealed that PCSK9 may cause poor prognosis of ASCVDs by activating the inflammatory response and promoting the process of thrombosis and cell death independent of its lipid-regulatory function, yet the underlying mechanisms still need to be further clarified. In patients with ASCVDs who are intolerant to statins or whose plasma LDL-C levels fail to descend to the target value after treatment with high-dose statins, PCSK9 inhibitors often improve their clinical outcomes. Here, we summarize the biological characteristics and functional mechanisms of PCSK9, highlighting its immunoregulatory function. We also discuss the effects of PCSK9 on common ASCVDs.
Semi-universal geo-crack detection by machine learning
Authors: Shi Yongxiang , Ballesio Marco , Johansen Kasper , Trentman Daniel , Huang Yunsong , McCabe Matthew F. , Bruhn Ronald , Schuster Gerard
Published: March 2023

Introduction: Cracks are a key feature that determines the structural integrity of rocks, and their angular distribution can be used to determine the local or regional stress patterns. The temporal growth of cracks can be monitored in order to predict impending failures of materials or structures such as a weakened dam. Thus, cracks and their spatial-temporal distributions should be automatically monitored for assessing their structural integrity, the associated stress patterns and their potential for failure.

Method: We show that the U-Net convolutional neural network, semantic segmentation and transfer learning can be used to accurately detect cracks in drone photos of sedimentary massifs. In this case, the crack distributions are used to assess the safest areas for tunnel excavation. Compared to the coarse performance of ridge detection, the U-Net accuracy in identifying cracks in images can be as high as 98% when evaluated against human identification, which is sufficient for assessing the general crack properties of the rock faces for the engineering project.

Result: Based on approximately 100 h of manual cracks labeling in 127 drone photos and 20 h of network training, the U-Net was able to successfully detect cracks in 23,845 high-resolution photographs in less than 22 h using two Nvidia V100 GPUs. Meanwhile, the network was able to detect more than 80% of the observable cracks of a volcanic outcrop in Idaho without additional training. With a modest amount of extra labeling on photos of the volcanic outcrop and transfer training, we found that the accuracy significantly improved. The surprising outcome of this research is that the U-Net crack detector laboriously trained on photos of sedimentary rocks can also be effectively applied to photos of volcanic rock faces. This can be important for real-time assessment of geological hazards and lithology information for dam inspection and planetary exploration by autonomous vehicles. For another application, we accurately detected fractures and faults with a scale of tens of kilometers from Martian photographs.

Conclusions: In summary, our methodology of using CNN with transfer training suggests that it can be used as a semi-universal detector of cracks in across a range of diverse geological settings.
Anthropogenic heat due to energy consumption contributes to cooler and wetter summers in Southwest China
Authors: Zhang Mengyi , Zhao Jingchuan , Chen Bing , Zheng Yuhao , Lu Mingwei , Wu Xue , Yang Huiyi , Liu Xiaohong , Wu Chenglai , Zhang Meigen , Zhao Xiaoqing , Jinlin Zha , Luo Tao , Fan Wenxuan , Shi Guangyu
Published: March 2023

Anthropogenic heat release is the release of heat generated by anthropogenic energy consumption. The regional mean Anthropogenic heat release flux in Southwest China grew quickly from 0.06 Wm-2 in 1992 to a peak of 0.37 Wm-2 in 2019. This study examines the climatic effects and feedbacks of Anthropogenic heat release due to energy consumption in Southwest China during the boreal summer using simulations from the Community Earth System Model. The modeling results show that Anthropogenic heat release impacts on the lower-troposphere stability and affects large-scale atmospheric circulation in Southwest China, which transports more water vapor and consequently increases the humidity and low cloud cover in Southwest China. This effect impacts the energy balance at the surface by reducing the amount of incoming shortwave radiation that reaches the ground. Anthropogenic heat release decreases the average 2-m air temperature in Southwest China by 0.10 ± 0.01 K (1σ uncertainty) and also decreases the minimum and maximum air temperatures in Southwest China as well. Anthropogenic heat release contributes to cooler and wetter summers in Southwest China. The results show that Anthropogenic heat release is a non-negligible factor that impacts the climate of Southwest China. This study improves our understanding of the climate change resulting from human activities in Southwest China.
Progesterone exerts a neuroprotective action in a Parkinson’s disease human cell model through membrane progesterone receptor α (mPRα/PAQR7)
Authors: Castelnovo Luca F. , Thomas Peter
Published: March 2023

Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide, and current treatment options are unsatisfactory on the long term. Several studies suggest a potential neuroprotective action by female hormones, especially estrogens. The potential role of progestogens, however, is less defined, and no studies have investigated the potential involvement of membrane progesterone receptors (mPRs). In the present study, the putative neuroprotective role for mPRs was investigated in SH-SY5Y cells, using two established pharmacological treatments for cellular PD models, 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenylpyridinium (MPP+). Our results show that both the physiologic agonist progesterone and the specific mPR agonist Org OD 02-0 were effective in reducing SH-SY5Y cell death induced by 6-OHDA and MPP+, whereas the nuclear PR agonist promegestone (R5020) and the GABAA receptor agonist muscimol were ineffective. Experiments performed with gene silencing technology and selective pharmacological agonists showed that mPRα is the isoform responsible for the neuroprotective effects we observed. Further experiments showed that the PI3K-AKT and MAP kinase signaling pathways are involved in the mPRα-mediated progestogen neuroprotective action in SH-SY5Y cells. These findings suggest that mPRα could play a neuroprotective role in PD pathology and may be a promising target for the development of therapeutic strategies for PD prevention or management.
Liver-bone crosstalk in non-alcoholic fatty liver disease: Clinical implications and underlying pathophysiology
Authors: Zhao Jiahui , Lei Hongyan , Wang Tianyi , Xiong Xuelian
Published: March 2023

Osteoporosis is a common complication of many types of chronic liver diseases (CLDs), such as cholestatic liver disease, viral hepatitis, and alcoholic liver disease. Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent metabolic liver disease, affecting almost one third of adults around the world, and is emerging as the dominant cause of CLDs. Liver serves as a hub for nutrient and energy metabolism in the body, and its crosstalk with other tissues, such as adipose tissue, heart, and brain, has been well recognized. However, much less is known about the crosstalk that occurs between the liver and bone. Moreover, the mechanisms by which CLDs increase the risk for osteoporosis remain unclear. This review summarizes the latest research on the liver–bone axis and discusses the relationship between NAFLD and osteoporosis. We cover key signaling molecules secreted by liver, such as insulin-like growth factor-1 (IGF-1), fibroblast growth factor 21 (FGF21), insulin-like growth factor binding protein 1 (IGFBP1), fetuin-A, tumor necrosis factor-alpha (TNF-α), and osteopontin (OPN), and their relevance to the homeostasis of bone metabolism. Finally, we consider the disordered liver metabolism that occurs in patients with NAFLD and how this disrupts signaling to the bone, thereby perturbing the balance between osteoclasts and osteoblasts and leading to osteoporosis or hepatic osteodystrophy (HOD).
Optimal capacity configuration of the wind-storage combined frequency regulation system considering secondary frequency drop
Authors: Li Dongdong , Wan Rui , Xu Bo , Yao Yin , Dong Nan , Zhang Xianming
Published: March 2023

With wind power integrated into the power system on a large scale, the system has become vulnerable to the frequency stability issue. The battery energy storage system (BESS) is considered the key solution to improving the system frequency regulation performance due to its fast response ability. Furthermore, the construction of wind-storage combined frequency regulation systems has been developed for many years, in which the optimal capacity configuration of the wind-storage system is getting more attention. However, the secondary frequency drop (SFD) caused by wind turbines (WTs) participating in primary frequency regulation (PFR) is neglected in most existing capacity configurations, which is worthy of further study. In this paper, the optimal capacity of the wind-storage combined frequency regulation system is studied from the perspective of SFD. The time-domain expressions of two-stage system frequency response considering SFD are derived based on the wind-storage combined frequency regulation model. Next, considering the technical and economic characteristics of wind-storage combined frequency regulation, an optimization model of the energy storage capacity configuration is established with the objective of minimizing the sum of the maximum frequency deviations in two stages and the energy storage cost. The optimization model is solved by the multi-objective salp swarm algorithm (MSSA) to obtain the setting value of wind-storage combined frequency regulation parameters and the optimal energy storage capacity. The effectiveness of the proposed method is verified in MATLAB. The simulation results show that the proposed model can effectively improve the frequency regulation effect of the system and ensure the optimal capacity configuration with better economy.
phenoC++: An open-source tool for retrieving vegetation phenology from satellite remote sensing data
Authors: Ruan Yongjian , Ruan Baozhen , Xin Qinchuan , Liao Xi , Jing Fengrui , Zhang Xinchang
Published: March 2023

Satellite-retrieved vegetation phenology has great potential for application in characterizing seasonal and annual land surface dynamics. However, obtaining regional-scale vegetation phenology from satellite remote sensing data often requires extensive data processing and computation, which makes the accurate and rapid retrieval of regional-scale phenology a challenge. To retrieve vegetation phenology from satellite remote sensing data, we developed an open-source tool called phenoC++, which uses parallel technology in C++. phenoC++ includes six common algorithms: amplitude threshold (AT), first-order derivative (FOD), second-order derivative (SOD), third-order derivative (TOD), relative change rate (RCR), and curvature change rate (CCR). We implemented the proposed phenoC++ and evaluated its performance on a site scale with PhenoCam-observed phenology metrics. The result shows that SOS derived from MODIS images by phenoC++ with six methods (i.e., AT, FOD, SOD, RCR, TOD, and CCR) obtained r-values of 0.75, 0.76, 0.75, 0.76, 0.64, and 0.67, and RMSE values of 21.36, 20.41, 22.38, 19.11, 33.56, and 32.14, respectively. Satellite-retrieved EOS by phenoC++ with six methods obtained r-values of 0.58, 0.59, 0.57, 0.56, 0.36, and 0.40, and RMSE values of 52.43, 46.68, 55.13, 49.46, 71.13, and 69.34, respectively. Using PhenoCam-observed phenology as a baseline, SOS retrieved by phenoC++ was superior to MCD12Q2, while EOS retrieved by phenoC++ was slightly inferior to that of MCD12Q2. Moreover, compared with MCD12Q2 on a regional scale, phenoC++-retrieved vegetation phenology yields more effective pixels. The innovative features of phenoC++ are 1) integrating six algorithms for retrieving SOS and EOS; 2) quickly processing data on a large scale with simple input startup parameters; 3) outputting phenology metrics in GeoTIFF format image, which is more convenient to use with other geospatial data. phenoC++ could aid in investigating and addressing large-scale phenology problems of the ecological environment.
The impact of FDI on haze pollution: “Pollution paradise” or “pollution halo?”--Spatial analysis of PM2.5 concentration raster data in 283 cities
Authors: Liu Yanbo , Guo Mingshun
Published: March 2023

This paper constructs a spatial econometric model based on the environmental Kuznets curve to examine the effect of foreign direct investment (FDI) on urban haze pollution in Chinese cities. The evidence from the raster data of PM2.5 concentration in MODIS and MISR shows that there is a significant spatial correlation between haze pollution and FDI in Chinese cities, which is expressed as a spatial spillover effect; FDI in Chinese mainland cities reduces haze pollution, which confirms the “pollution halo hypothesis”; using instrumental variable estimation, the above conclusions are still robust; The total effect of FDI on haze pollution is negative, and the total effect can be decomposed into scale effect, technology effect and structural effect; FDI increases urban haze pollution through scale effects and structural effect, but decreases urban haze pollution through technology effects; the environmental Kuznets curve exists in urban haze pollution in China, i.e., there is an inverted “U” relationship between economic development and haze pollution. This paper proposes that the establishment of inter-city linkage mechanism of haze control and the full play of the technical effect of FDI on haze pollution will help reduce urban haze pollution.
BIRDIE: A data pipeline to inform wetland and waterbird conservation at multiple scales
Authors: Cervantes Francisco , Altwegg Res , Strobbe Francis , Skowno Andrew , Visser Vernon , Brooks Michael , Stojanov Yvan , Harebottle Douglas M. , Job Nancy
Published: March 2023

Introduction

Efforts to collect ecological data have intensified over the last decade. This is especially true for freshwater habitats, which are among the most impacted by human activity and yet lagging behind in terms of data availability. Now, to support conservation programmes and management decisions, these data need to be analyzed and interpreted; a process that can be complex and time consuming. The South African Biodiversity Data Pipeline for Wetlands and Waterbirds (BIRDIE) aims to help fast and efficient information uptake, bridging the gap between raw ecological datasets and the information final users need.

Methods

BIRDIE is a full data pipeline that takes up raw data, and estimates indicators related to waterbird populations, while keeping track of their associated uncertainty. At present, we focus on the assessment of species abundance and distribution in South Africa using two citizen-science bird monitoring datasets, namely: the African Bird Atlas Project and the Coordinated Waterbird Counts. These data are analyzed with occupancy and state-space models, respectively. In addition, a suite of environmental layers help contextualize waterbird population indicators, and link these to the ecological condition of the supporting wetlands. Both data and estimated indicators are accessible to end users through an online portal and web services.

Results and discussion

We have designed a modular system that includes tasks, such as: data cleaning, statistical analysis, diagnostics, and computation of indicators. Envisioned users of BIRDIE include government officials, conservation managers, researchers and the general public, all of whom have been engaged throughout the project. Acknowledging that conservation programmes run at multiple spatial and temporal scales, we have developed a granular framework in which indicators are estimated at small scales, and then these are aggregated to compute similar indicators at broader scales. Thus, the online portal is designed to provide spatial and temporal visualization of the indicators using maps, time series and pre-compiled reports for species, sites and conservation programmes. In the future, we aim to expand the geographical coverage of the pipeline to other African countries, and develop more indicators specific to the ecological structure and function of wetlands.
Epigenetics and immune cells in medulloblastoma
Authors: Gorini Francesca , Miceli Marco , de Antonellis Pasqualino , Amente Stefano , Zollo Massimo , Ferrucci Veronica
Published: March 2023

Medulloblastoma (MB) is a highly malignant childhood tumor of the cerebellum. Transcriptional and epigenetic signatures have classified MB into four molecular subgroups, further stratified into biologically different subtypes with distinct somatic copy-number aberrations, driver genes, epigenetic alterations, activated pathways, and clinical outcomes. The brain tumor microenvironment (BTME) is of importance to regulate a complex network of cells, including immune cells, involved in cancer progression in brain malignancies. MB was considered with a “cold” immunophenotype due to the low influx of immune cells across the blood brain barrier (BBB). Recently, this assumption has been reconsidered because of the identification of infiltrating immune cells showing immunosuppressive phenotypes in the BTME of MB tumors. Here, we are providing a comprehensive overview of the current status of epigenetics alterations occurring during cancer progression with a description of the genomic landscape of MB by focusing on immune cells within the BTME. We further describe how new immunotherapeutic approaches could influence concurring epigenetic mechanisms of the immunosuppressive cells in BTME. In conclusion, the modulation of these molecular genetic complexes in BTME during cancer progression might enhance the therapeutic benefit, thus firing new weapons to fight MB.
Deficiency of endothelial FGFR1 signaling via upregulation of ROCK2 activity aggravated ALI/ARDS
Authors: Deng Yue , Huang Xingming , Hu Yan , Zhong Weiting , Zhang Hua , Mo Chunheng , Wang Hongjun , Ding Bi-Sen , Wang Chen
Published: March 2023

Vascular leakage and inflammation are pathological hallmarks of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Endothelial cells (ECs) serve as a semipermeable barrier and play a key role in disease progression. It is well known that fibroblast growth factor receptor 1 (FGFR1) is required for maintaining vascular integrity. However, how endothelial FGFR1 functions in ALI/ARDS remains obscure. Here, we revealed that conditional deletion of endothelial FGFR1 aggravated LPS-induced lung injury, including inflammation and vascular leakage. Inhibition of its downstream Rho-associated coiled-coil–forming protein kinase 2 (ROCK2) by AAV Vec-tie-shROCK2 or its selective inhibitor TDI01 effectively attenuated inflammation and vascular leakage in a mouse model. In vitro, TNFα-stimulated human umbilical vein endothelial cells (HUVECs) showed decreased FGFR1 expression and increased ROCK2 activity. Furthermore, knockdown of FGFR1 activated ROCK2 and thus promoted higher adhesive properties to inflammatory cells and higher permeability in HUVECs. TDI01 effectively suppressed ROCK2 activity and rescued the endothelial dysfunction. These data demonstrated that the loss of endothelial FGFR1 signaling mediated an increase in ROCK2 activity, which led to an inflammatory response and vascular leakage in vivo and in vitro. Moreover, inhibition of ROCK2 activity by TDI01 provided great value and shed light on clinical translation.
Alterations of the fecal and vaginal microbiomes in patients with systemic lupus erythematosus and their associations with immunological profiles
Authors: Ling Zongxin , Cheng Yiwen , Gao Jie , Lei Wenhui , Yan Xiumei , Hu Xiaogang , Shao Li , Liu Xia , Kang Runfang
Published: March 2023

Background

Exploring the human microbiome in multiple body niches is beneficial for clinicians to determine which microbial dysbiosis should be targeted first. We aimed to study whether both the fecal and vaginal microbiomes are disrupted in SLE patients and whether they are correlated, as well as their associations with immunological features.

Methods

A group of 30 SLE patients and 30 BMI-age-matched healthy controls were recruited. Fecal and vaginal samples were collected, the 16S rRNA gene was sequenced to profile microbiomes, and immunological features were examined.

Results

Distinct fecal and vaginal bacterial communities and decreased microbial diversity in feces compared with the vagina were found in SLE patients and controls. Altered bacterial communities were found in the feces and vaginas of patients. Compared with the controls, the SLE group had slightly lower gut bacterial diversity, which was accompanied by significantly higher bacterial diversity in their vaginas. The most predominant bacteria differed between feces and the vagina in all groups. Eleven genera differed in patients’ feces; for example, Gardnerella and Lactobacillus increased, whereas Faecalibacterium decreased. Almost all the 13 genera differed in SLE patients’ vaginas, showing higher abundances except for Lactobacillus. Three genera in feces and 11 genera in the vagina were biomarkers for SLE patients. The distinct immunological features were only associated with patients’ vaginal microbiomes; for example, Escherichia−Shigella was negatively associated with serum C4.

Conclusions

Although SLE patients had fecal and vaginal dysbiosis, dysbiosis in the vagina was more obvious than that in feces. Additionally, only the vaginal microbiome interacted with patients’ immunological features.
The role of the complosome in health and disease
Authors: Xiao Fang , Guo Jixu , Tomlinson Stephen , Yuan Guandou , He Songqing
Published: March 2023

The complement system is one of the immune system’s oldest defense mechanisms and is historically regarded as a liver-derived and serum-active innate immune system that ‘complements’ cell-mediated and antibody-mediated immune responses against pathogens. However, the complement system is now recognized as a central component of both innate and adaptive immunity at both the systemic and local tissue levels. More findings have uncovered novel activities of an intracellularly active complement system—the complosome—that have shifted established functional paradigms in the field. The complosome has been shown to play a critical function in regulating T cell responses, cell physiology (such as metabolism), inflammatory disease processes, and cancer, which has amply proved its immense research potential and informed us that there is still much to learn about this system. Here, we summarize current understanding and discuss the emerging roles of the complosome in health and disease.
Association of rheumatoid arthritis with bronchial asthma and asthma-related comorbidities: A population-based national surveillance study
Authors: Kim Jung Gon , Kang Jiyeon , Lee Joo-Hyun , Koo Hyeon-Kyoung
Published: March 2023

Background

The aim of this study was to investigate the impact of rheumatoid arthritis (RA) on the prevalence of bronchial asthma and asthma-related comorbidities. We also aimed to identify the influence of RA on interrelationship between asthma and asthma-related comorbidities.

Methods

From the Korean National Health and Nutrition Examination Survey, participants >40 years of age who completed questionnaires and spirometry tests were enrolled. Patient data on RA, asthma, allergic rhinitis, atopic dermatitis, chronic obstructive pulmonary disease (COPD), sinusitis, otitis media, and body mass index (BMI) were collected. Logistic regression and network analyses were performed.

Results

A total of 14,272 subjects were enrolled, among which, 334 (2.4%) had RA. RA was significantly associated with asthma (OR 2.32; 95% CI 1.51–3.57), allergic rhinitis (OR 1.51; 95% CI 1.08–2.10), and sinusitis (OR 1.64; 95% CI 1.08–2.50). The network analysis of total patients revealed a positive interrelationship between asthma and allergic rhinitis, sinusitis, otitis media, atopic dermatitis, BMI, and RA. The interrelationship between asthma and sinusitis was stronger in the RA group. Of note, the relationship between asthma and BMI was distinctively found only in the RA group (r = 0.214, P < 0.05). In patients with asthma, the prevalence of obesity was 64% in the presence of RA, and 40% in the absence of RA (P = 0.034).

Conclusion

This study supports the positive association of RA with asthma, allergic rhinitis, and sinusitis. Our analysis suggests a notable interrelationship between the presence of asthma and higher BMI values in patients with RA, indicating that asthma is more obesity-related in patients with RA.
Case report: Thyroid sarcoidosis as a rare localization of the disease: Report of two cases and review of the literature
Authors: Kašiković Lečić Svetlana , Javorac Jovan , Lovrenski Aleksandra , Đokić Jelena , Sazdanić Velikić Danica , Živanović Dejan
Published: March 2023

Sarcoidosis is a multi-systemic disease of unknown etiology that is characterized by the formation of non-necrotizing and non-caseating granulomas in affected organs. Sarcoidosis granulomas can form in any organ, but the lungs and intrathoracic lymph nodes are the most commonly affected. Thyroid involvement in sarcoidosis is very rare, with prevalence estimates of 1–4.5% in case series of autopsied patients with systemic sarcoidosis. It is extremely rare for sarcoidosis to occur solely in the thyroid gland, but it is usually associated with the involvement of other organs, primarily the lungs and lymph nodes. Typical manifestations are diffuse goiter and solitary or multiple thyroid nodules. Thyroid function remains intact in the majority of cases, but sometimes it can result in hypothyroidism or hyperthyroidism. The diagnosis can be made after fine needle aspiration cytology, but usually it is diagnosed as an incidental finding while analyzing thyroidectomy tissue or during autopsy. Oral steroids are the cornerstone of thyroid sarcoidosis management, along with specific endocrinological treatment on some occasions. Given that we found only 71 reports of thyroid sarcoidosis available in the literature, we created and analyzed a cohort of 24 patients with thyroid sarcoidosis who were described in the literature in the 21st century and reported two additional cases of thyroid sarcoidosis.
Platelet, a key regulator of innate and adaptive immunity
Authors: Yan Cheng , Wu Haojie , Fang Xianchun , He Junji , Zhu Feng
Published: March 2023

Platelets, anucleate blood components, represent the major cell type involved in the regulation of hemostasis and thrombosis. In addition to performing haemostatic roles, platelets can influence both innate and adaptive immune responses. In this review, we summarize the development of platelets and their functions in hemostasis. We also discuss the interactions between platelet products and innate or adaptive immune cells, including neutrophils, monocytes, macrophages, T cells, B cells and dendritic cells. Activated platelets and released molecules regulate the differentiation and function of these cells via platelet-derived receptors or secreting molecules. Platelets have dual effects on nearly all immune cells. Understanding the exact mechanisms underlying these effects will enable further application of platelet transfusion.
Algal amendment enhances biogenic methane production from coals of different thermal maturity
Authors: Platt George A. , Davis Katherine J. , Schweitzer Hannah D. , Smith Heidi J. , Fields Matthew W. , Barnhart Elliott P. , Gerlach Robin
Published: March 2023

The addition of small amounts of algal biomass to stimulate methane production in coal seams is a promising low carbon renewable coalbed methane enhancement technique. However, little is known about how the addition of algal biomass amendment affects methane production from coals of different thermal maturity. Here, we show that biogenic methane can be produced from five coals ranging in rank from lignite to low-volatile bituminous using a coal-derived microbial consortium in batch microcosms with and without algal amendment. The addition of 0.1 g/l algal biomass resulted in maximum methane production rates up to 37 days earlier and decreased the time required to reach maximum methane production by 17–19 days when compared to unamended, analogous microcosms. Cumulative methane production and methane production rate were generally highest in low rank, subbituminous coals, but no clear association between increasing vitrinite reflectance and decreasing methane production could be determined. Microbial community analysis revealed that archaeal populations were correlated with methane production rate (p = 0.01), vitrinite reflectance (p = 0.03), percent volatile matter (p = 0.03), and fixed carbon (p = 0.02), all of which are related to coal rank and composition. Sequences indicative of the acetoclastic methanogenic genus Methanosaeta dominated low rank coal microcosms. Amended treatments that had increased methane production relative to unamended analogs had high relative abundances of the hydrogenotrophic methanogenic genus Methanobacterium and the bacterial family Pseudomonadaceae. These results suggest that algal amendment may shift coal-derived microbial communities towards coal-degrading bacteria and CO2-reducing methanogens. These results have broad implications for understanding subsurface carbon cycling in coal beds and the adoption of low carbon renewable microbially enhanced coalbed methane techniques across a diverse range of coal geology.
White matter and gray matter changes related to cognition in community populations
Authors: Li Wen-Xin , Yuan Jing , Han Fei , Zhou Li-Xin , Ni Jun , Yao Ming , Zhang Shu-Yang , Jin Zheng-Yu , Cui Li-Ying , Zhai Fei-Fei , Zhu Yi-Cheng
Published: March 2023

Objective

Further studies are needed to improve the understanding of the pathological process underlying cognitive impairments. The purpose of this study is to investigate the global and topographic changes of white matter integrity and cortical structure related to cognitive impairments in a community-based population.

Methods

A cross-sectional analysis was performed based on 995 subjects (aged 56.8 ± 9.1 years, 34.8% males) from the Shunyi study, a community-dwelling cohort. Cognitive status was accessed by a series of neurocognitive tests including Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), category Verbal Fluency Test (VFT), Digit Span Test (DST), and Trail Making Tests A and B (TMT-A and TMT-B). Structural and diffusional MRI data were acquired. White matter integrity was assessed using fractional anisotropy (FA), mean diffusivity (MD), and peak width of skeletonized mean diffusivity (PSMD). Cortical surface area, thickness, and volume were measured using Freesurfer. Probabilistic tractography was further conducted to track the white matter fibers connecting to the cortical regions related to cognition. General linear models were used to investigate the association between brain structure and cognition.

Results

Global mean FA and MD were significantly associated with performances in VFT (FA, β 0.119, p < 0.001; MD, β −0.128, p < 0.001). Global cortical surface area, thickness, and volume were not related to cognitive scores. In tract-based spatial statistics analysis, disruptive white matter integrity was related to cognition impairment, mainly in visuomotor processing speed, semantic memory, and executive function (TMT-A and VFT), rather than verbal short-term memory and working memory (DST). In the whole brain vertex-wise analysis, surface area in the left orbitofrontal cortex, right posterior-dorsal part of the cingulate gyrus, and left central sulcus were positively associated with MMSE and MoCA scores, and the association were independent of the connecting white matter tract.

Conclusion

Disrupted white matter integrity and regional cortical surface area were related to cognition in community-dwelling populations. The associations of cortical surface area and cognition were independent of the connecting white matter tract.
Factors impacting quality of life in multiple system atrophy
Authors: Ali Nabila , Nesspor Vanessa , Bang Jee , Scholz Sonja W. , Pantelyat Alexander
Published: March 2023

Background

Multiple system atrophy (MSA) is an atypical parkinsonian disorder marked by autonomic dysfunction, parkinsonism, cerebellar dysfunction, and poor response to dopaminergic medications such as levodopa. Patient-reported quality of life is an important benchmark for clinicians and clinical trials. The Unified Multiple System Atrophy Rating Scale (UMSARS) allows healthcare providers to rate and assess MSA progression. The MSA-QoL questionnaire is a health-related quality of life scale intended to provide patient-reported outcome measures. In this article, we investigated inter-scale correlations between the MSA-QoL and UMSARS to determine factors impacting the quality of life of patients with MSA.

Methods

Twenty patients at the Johns Hopkins Atypical Parkinsonism Center's Multidisciplinary Clinic with a diagnosis of clinically probable MSA and who filled out the MSA-QoL and UMSARS questionnaires within 2 weeks of each other were included. Inter-scale correlations between MSA-QoL and UMSARS responses were examined. Linear regressions were also performed to examine relationships between both scales.

Results

Significant inter-scale correlations were found between the MSA-QoL and UMSARS, both between MSA-QoL total score and UMSARS Part I subtotal scores and for individual scale items. There were no significant correlations between MSA-QoL life satisfaction rating and UMSARS subtotal scores or any specific UMSARS items. Linear regression analysis found significant associations between MSA-QoL total score and UMSARS Part I and total scores, and between MSA-QoL life satisfaction rating and UMSARS Part I, Part II, and total scores (after adjustment for age).

Conclusions

Our study demonstrates significant inter-scale correlations between MSA-QoL and UMSARS, particularly relating to activities of daily living and hygiene. MSA-QoL total score and UMSARS Part I subtotal scores, which assess patients' functional status, were significantly correlated. The lack of significant associations between MSA-QoL life satisfaction rating and any UMSARS item suggests there may be aspects to quality of life that are not fully captured by this assessment. Larger cross-sectional and longitudinal analyses utilizing UMSARS and MSA-QoL are warranted and modification of the UMSARS should be considered.
The effects of COVID-19 on the vestibular system
Authors: Zaubitzer Lena , Ludwig Sonja , Berkemann Michelle , Walter Beatrice , Jungbauer Frederic , Held Valentin , Hegemann Stefan C. A. , Rotter Nicole , Schell Angela
Published: March 2023

Introduction

The symptoms and severity of SARS-CoV-2 infection vary greatly across the spectrum, from asymptomatic infection to severe pneumonia with acute respiratory distress syndrome and even death. Dizziness is a frequently reported symptom of SARS-CoV-2 viral infection. However, the extent to which this symptom results from the effect of SARS-CoV-2 on the vestibular system remains unclear.

Materials and methods

In the present single-center, prospective cohort study, patients with a previous SARS-CoV-2 infection underwent a vestibular assessment consisting of the Dizziness Handicap Inventory to assess dizziness during and after infection, a clinical examination, the video head impulse test, and the subjective visual vertical test. When the subjective visual vertical test result was abnormal, vestibular-evoked myogenic potentials were performed. Vestibular testing results were compared to pre-existing normative data of healthy controls. In addition, we performed a retrospective data analysis of patients admitted to hospital presenting with acute symptoms of dizziness who were also diagnosed with acute SARS-CoV-2 infection.

Results

A total of 50 participants have been enrolled. During and after the SARS-CoV-2 infection, women were significantly more likely than men to suffer from dizziness. A significantly reduced semicircular canal or otolith function was not observed in either women or men. Acute SARS-CoV-2 infection was diagnosed in nine patients who presented to the emergency room with acute vestibular syndrome. Six of the patients exhibited acute unilateral peripheral vestibulopathy upon diagnosis. A different patient was diagnosed with vestibular migraine, and two individuals had a posterior inferior cerebellar artery infarct revealed by magnetic resonance imaging.

Discussion/conclusion

Overall, a persisting structural affection of the vestibular system by SARS-CoV-2 seems to be unlikely and could not be confirmed by vHIT, SVV, and VEMPS in our study. It seems possible but unlikely that SARS-CoV-2 induces acute vestibulopathy. Nevertheless, dizziness is a common symptom in patients with COVID-19, which should be taken and worked through seriously.
Diagnosis and treatment evaluation in patients with spontaneous intracranial hypotension
Authors: Mehta Dwij , Cheema Sanjay , Davagnanam Indran , Matharu Manjit
Published: March 2023

Spontaneous intracranial hypotension is characterized by an orthostatic headache and audiovestibular symptoms alongside a myriad of other non-specific symptoms. It is caused by an unregulated loss of cerebrospinal fluid at the spinal level. Indirect features of CSF leaks are seen on brain imaging as signs of intracranial hypotension and/or CSF hypovolaemia as well as a low opening pressure on lumbar puncture. Direct evidence of CSF leaks can frequently, but not invariably, be observed on spinal imaging. The condition is frequently misdiagnosed due to its vague symptoms and a lack of awareness of the condition amongst the non-neurological specialities. There is also a distinct lack of consensus on which of the many investigative and treatment options available to use when managing suspected CSF leaks. The aim of this article is to review the current literature on spontaneous intracranial hypotension and its clinical presentation, preferred investigation modalities, and most efficacious treatment options. By doing so, we hope to provide a framework on how to approach a patient with suspected spontaneous intracranial hypotension and help minimize diagnostic and treatment delays in order to improve clinical outcomes.
Research progress on the therapeutic effects of polysaccharides on non-alcoholic fatty liver diseases
Authors: Hu Yu-long , Ma Qiaoli , Dong Xiaoqiang , Kong Yuanfang , Cai Juntao , Li Jieming , Dong Chunhong
Published: March 2023

Non-alcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease and is a leading cause of cirrhosis and hepatocellular carcinoma. Due to its complex pathophysiology, there is currently no approved therapy. Polysaccharide, a kind of natural product, possesses a wide range of pharmacological activities. Numerous preclinical studies have confirmed that polysaccharides could interfere with the occurrence and development of NAFLD at multiple interrelated levels, such as improvement of glucose and lipid metabolism, antioxidation, anti-inflammation, and regulation of gut-liver axis, thus showing great potential as novel anti-NAFLD drugs. In this paper, we reviewed the polysaccharides with anti-NAFLD effect in recent years, and also systematically analyzed their possible pharmacological mechanisms.
In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis
Authors: Fan Di , Zhang Chengkai , Luo Qi , Li Baowang , Ai Lin , Li Deling , Jia Wang
Published: March 2023

Introduction

Integrin αvβ6, which is upregulated in malignancies and remains absent or weak in normal tissue, is a promising target in molecular imaging therapeutics. In vivo imaging of integrin αvβ6 could therefore be valuable for early tumor detection and intraoperative guidance.

Methods

In this study, integrin αvβ6-targeting probe G2-SFLAP3 was labeled with near-infrared (NIR) dye Cy5.5 or radioisotope 68Ga. The resulting probes were evaluated in integrin αvβ6-positive A549 and αvβ6-negative H1703 xenograft mice models.

Results

The cellar uptake of G2-SFLAP3-Cy5.5 was consistent with the expression of integrin αvβ6. Both subcutaneous and brain metastatic A549 tumors could be clearly visualized by NIR fluorescent imaging of G2-SFLAP3-Cy5.5. A549 tumors demonstrated the highest G2-SFLAP3-Cy5.5 accumulation at 4h post-injection (p.i.) and remain detectable at 84h p.i. The fluorescent signal of G2-SFLAP3-Cy5.5 was significantly reduced in H1703 and A549-blocking groups. Consistently, small-animal PET imaging showed tumor-specific accumulation of 68Ga-DOTA-G2-SFLAP3.

Discussion

G2-SFLAP3 represents a promising agent for noninvasive imaging of non-small cell lung cancer (NSCLC) and brain metastases.