bluuebunny/crossref_metadata_2025 huggingface dataset:
Parental thermal environment controls the offspring phenotype in Brook charr (Salvelinus fontinalis): insights from a transcriptomic study
Authors: Banousse Ghizlane , Normandeau Eric , Semeniuk Christina , Bernatchez Louis , Audet Céline
Published: March 2024

Brook charr is a cold-water species which is highly sensitive to increased water temperatures, such as those associated with climate change. Environmental variation can potentially induce phenotypic changes that are inherited across generations, for instance, via epigenetic mechanisms. Here, we tested whether parental thermal regimes (intergenerational plasticity) and offspring-rearing temperatures (within-generational plasticity) modify the brain transcriptome of Brook charr progeny (fry stage). Parents were exposed to either cold or warm temperatures during final gonad maturation and their progeny were reared at 5 or 8 °C during the first stages of development. Illumina Novaseq6000 was used to sequence the brain transcriptome at the yolk sac resorption stage. The number of differentially expressed genes was very low when comparing fry reared at different temperatures (79 differentially expressed genes). In contrast, 9,050 differentially expressed genes were significantly differentially expressed between fry issued from parents exposed to either cold or warm temperatures. There was a significant downregulation of processes related to neural and synaptic activity in fry originating from the warm parental group vs fry from the cold parental one. We also observed significant upregulation of DNA methylation genes and of the most salient processes associated with compensation to warming, such as metabolism, cellular response to stress, and adaptive immunity.
Early-Life Exposure to War and Later-Life Physical Functional Health
Authors: Haas Steven A , Ramirez Daniel
Published: March 2024

Objectives

A growing body of research shows that early-life exposure to war has adverse effects on later-life health. Research has emphasized the importance of exposure timing implicating domain-specific developmental processes and associated critical/sensitive periods. This study looks at the impacts of early childhood war exposure and the repercussions for later-life physical and functional health, with a focus on time of exposure as a source of variability.

Methods

We use residential histories from the Survey of Health Ageing, and Retirement in Europe linked to external data on the location and timing of hostilities to examine the impact of early-life exposure to World War II on later-life physical and functional health.

Results

Exposure to war increases the risk of objective (grip strength, chair rise, and peak expiratory flow) and self-reported (mobility limitations and activities of daily living) measures of functional health. Effects are especially pronounced for those born during the war and for those with more prolonged exposures. There is little evidence that the impact of war is mediated by war-related hardships, socioeconomic conditions, health behaviors, or adult chronic disease.

Discussion

Our results suggest early-life exposure to war has a lasting impact on physical functional health. Exposure appears to largely operate via direct effects, indicative of altered initial development of physical capacity in early life. Because exposure was so pervasive among some cohorts of older individuals, understanding the health of present older European populations requires wrestling with the residual consequences of wartime exposure at the start of their lives.
New Genomic Techniques and Intellectual Property Law: Challenges and Solutions for the Plant Breeding Sector ‒ Position Statement of the Max Planck Institute for Innovation and Competition
Authors: Kim Daria , Kock Michael A , Lamping Matthias , Batista Pedro Henrique D , Hilty Reto M , Slowinski Peter R , Steinhart Miriam
Published: March 2024

On 5 July 2023, the European Commission proposed a regulation aiming to ease the requirements for the marketing authorisation of plants obtained through certain new genomic techniques (NGTs) within the European Union (EU). While NGTs are expected to become more attractive to breeders and farmers, the complexity of the intellectual property (IP) landscape surrounding these techniques and resulting products may negatively impact technology diffusion and innovation. Given numerous concerns related to IP protection for NGTs and NGT-derived plants, this Position Statement from the Max Planck Institute for Innovation and Competition presents a set of policy recommendations for facilitating access to and utilisation of IP-protected NGTs and NGT-derived products in the breeding sector.
Microfluidic sperm sorting selects a subpopulation of high-quality sperm with a higher potential for fertilization
Authors: Sheibak Nadia , Amjadi Fatemehsadat , Shamloo Amir , Zarei Fatemeh , Zandieh Zahra
Published: March 2024

STUDY QUESTION

Is a microfluidic sperm sorter (MSS) able to select higher quality sperm compared to conventional methods?

SUMMARY ANSWER

The MSS selects sperm with improved parameters, lower DNA fragmentation, and higher fertilizing potential.

WHAT IS KNOWN ALREADY

To date, the few studies that have compared microfluidics sperm selection with conventional methods have used heterogeneous study population and have lacked molecular investigations.

STUDY DESIGN, SIZE, DURATION

The efficiency of a newly designed MSS in isolating high-quality sperm was compared to the density-gradient centrifugation (DGC) and swim-up (SU) methods, using 100 semen samples in two groups, during 2023–2024.

PARTICIPANTS/MATERIALS, SETTING, METHODS

Semen specimens from 50 normozoospermic and 50 non-normozoospermic men were sorted using MSS, DGC, and SU methods to compare parameters related to the quality and fertilizing potential of sperm. The fertilizing potential of sperm was determined by measurement of phospholipase C zeta (PLCζ) and post-acrosomal sheath WW domain-binding protein (PAWP) expression using flow cytometry, and the chromatin dispersion test was used to assess sperm DNA damage.

MAIN RESULTS AND THE ROLE OF CHANCE

In both normozoospermic and non-normozoospermic groups, the MSS-selected sperm with the highest progressive motility, PLCζ positive expression and PLCζ and PAWP fluorescence intensity the lowest non-progressive motility, and minimal DNA fragmentation, compared to sperm selected by DGC and SU methods (P < 0.05).

LIMITATION, REASONS FOR CAUTION

The major limitations of our study were the low yield of sperm in the MSS chips and intentional exclusion of severe male factor infertility to yield a sufficient sperm count for molecular experiments; thus testing with severe oligozoospermic semen and samples with low count and motility is still required. In addition, due to ethical considerations, at present, it was impossible to use the sperm achieved from MSS in the clinic to assess the fertilization rate and further outcomes.

WIDER IMPLICATIONS OF THE FINDINGS

Our research presents new evidence that microfluidic sperm sorting may result in the selection of high-quality sperm from raw semen. This novel technology might be a key to improving clinical outcomes of assisted reproduction in infertile patients.

STUDY FUNDING/COMPETING INTEREST(S)

The study is funded by the Iran University of Medical Sciences and no competing interest exists.

TRIAL REGISTRATION NUMBER

N/A.
Crisis Leadership in the Time of Covid: Effects of Personality Traits on Response Speed
Authors: Oktay Sibel
Published: March 2024

Why were some countries faster than others at introducing COVID-19 mitigation policies? Existing research shows that leaders are central to defining and making sense of crises, which then shapes their governments’ crisis response. I argue that crisis-sense-making depends on leaders’ personality traits pertaining to how they perceive their power status, collect and process information, and interact with their environment, subsequently influencing their governments’ crisis response speed. I test this argument in the context of the European experience with COVID-19, combining an original Leadership Traits Analysis dataset of thirty-two leaders with the Oxford COVID-19 Government Response Tracker data. I show that leaders who were highly self-confident or exhibited a tendency to challenge constraints were significantly slower at introducing pandemic mitigation policies. Leaders who were open to information were significantly faster to do so. I also show that policy emulation between countries over time dissipated the slowing effects of the leaders’ need for power and conceptual complexity on response speed. The results present new and systematic evidence on how leader personality shaped pandemic policy-making, contributing to the literature on crisis policy-making and political leadership. They also offer science advisors food for thought on how to communicate with leaders.
Assessment of risk-adjusted mortality ratio (RAMR) in bloodstream infections using all-patient refined diagnosis-related groups (APR-DRGs)
Authors: Maestro De La Calle Guillermo , Vélez Jorge , Mateo Flores Javier , García Barrio Noelia , Orellana María Ángeles , Quirós-González Víctor , Lumbreras Bermejo Carlos , Bernal José Luis
Published: March 2024

Objectives

To calculate a risk-adjusted mortality ratio (RAMR) for bloodstream infections (BSIs) using all-patient refined diagnosis-related groups (APR-DRGs) and compare it with the crude mortality rate (CMR).

Methods

Retrospective observational study of prevalent BSI at our institution from January 2019 to December 2022. In-hospital mortality was adjusted with a binary logistic regression model adjusting for sex, age, admission type and mortality risk for the hospitalization episode according to the four severity levels of APR DRGs. The RAMR was calculated as the ratio of observed to expected in-hospital mortality, and the CMR was calculated as the proportion of deaths among all bacteraemia episodes.

Results

Of 2939 BSIs, 2541 were included: Escherichia coli (n = 1310), Klebsiella pneumoniae (n = 428), Pseudomonas aeruginosa (n = 209), Staphylococcus aureus (n = 498) and candidaemia (n = 96). A total of 436 (17.2%) patients died during hospitalization and 279 died within the first 14 days after the onset of BSI. Throughout the period, all BSI cases had a mortality rate above the expected adjusted mortality (RAMR value greater than 1), except for Escherichia coli (1.03; 95% CI 0.86–1.21). The highest overall RAMR values were observed for P. aeruginosa, Candida and S. aureus with 2.06 (95% CI 1.57–2.62), 1.99 (95% CI 1.3–2.81) and 1.8 (95% CI 1.47–2.16), respectively. The temporal evolution of CMR may differ from RAMR, especially in E. coli, where it was reversed.

Conclusions

RAMR showed higher than expected mortality for all BSIs studied except E. coli and provides complementary to and more clinically comprehensive information than CMR, the currently recommended antibiotic stewardship programme mortality indicator.
Investigation of the seasonal prevalence, phenotypic, and genotypic characteristics ofListeria monocytogenesin slaughterhouses in Burdur
Authors: Erol Zeki , Taşçı Fulya
Published: March 2024

Aim

This study examined Listeria monocytogenes isolates from two slaughterhouses in Burdur province, southern Turkey, over four seasons for antibiotic resistance, serogroups, virulence genes, in vitro biofilm forming capacity, and genetic relatedness.

Methods and results

Carcass (540) and environment-equipment surface (180) samples were collected from two slaughterhouses (S1, S2) for 1 year (4 samplings). Of the 89 (12.4%) positive isolates, 48 (53.9%) were from animal carcasses, and 41 (46.1%) from the environment-equipment surfaces. Autumn was the peak season for Listeria monocytogenes compared to summer and spring (P < 0.05). In addition, the most common serotype between seasons was 1/2c. Except for plcA and luxS genes, all isolates (100%) harbored inlA, inlC, inlJ, hlyA, actA, iap, flaA genes. Listeria monocytogenes isolates were identified as belonging to IIc (1/2c-3c; 68.5%), IVb (4b-4d-4e; 29.2%), and IIa (1/2a-3a; 2.2%) in the screening using multiplex polymerase chain reaction-based serogrouping test. A total of 65 pulsotypes and 13 clusters with at least 80% homology were determined by using pulsed field gel electrophoresis on samples that had been digested with ApaI. Thirty-four (38.2%) of the isolates were not resistant to any of the 14 antibiotics tested. The antibiotic to which the isolates showed the most resistance was rifampicin (44.9%). Serotype 1/2c was the most resistant serotype to antibiotics. Despite having biofilm-associated genes (inlA, inlB, actA, flaA, and luxS), a minority (11%) of isolates formed weak biofilm.

Conclusion

This study revealed seasonal changes prevalence of Listeria monocytogenes, particularly higher in autumn, posing a greater risk of meat contamination. Notably, Serotype 1/2c showed significant prevalence and antibiotic resistance. Indistinguishable isolates indicated cross-contamination, underscoring the importance of prioritized training for slaughterhouse personnel in sanitation and hygiene protocols.
Antimicrobial resistance, β-lactamase genotypes, and plasmid replicon types of Shiga toxin-producing Escherichia coli isolated from different animal hosts
Authors: Tomeh Rwida , Nemati Ali , Hashemi Tabar Gholamreza , Tozzoli Rosangela , Badouei Mahdi Askari
Published: March 2024

Aims

The primary objective of this study was to analyze antimicrobial resistance (AMR), with a particular focus on β-lactamase genotypes and plasmid replicon types of Shiga toxin-producing Escherichia coli (STEC) strains originating from various animal hosts.

Methods and results

A total of 84 STEC strains were isolated from cattle (n = 32), sheep/goats (n = 26), pigeons (n = 20), and wild animals (n = 6) between 2010 and 2018 in various regions of Iran. The Kirby-Bauer susceptibility test and multiple polymerase chain reaction (PCR) panels were employed to elucidate the correlation between AMR and plasmid replicon types in STEC isolates. The predominant replicon types were IncFIC and IncFIB in cattle (46.8%), IncFIC in sheep/goats (46.1%), IncA/C in pigeons (90%), and IncP in wild animals (50%). STEC of serogroups O113, O26, and O111 harbored the IncFIB (100%), IncI1 (80%), and IncFIC + IncA/C (100%) plasmids, respectively. A remarkable AMR association was found between ciprofloxacin (100%), neomycin (68.7%), and tetracycline (61.7%) resistance with IncFIC; amoxicillin + clavulanic acid (88.8%) and tetracycline (61.7%) with IncA/C; ciprofloxacin (100%) with IncFIB; fosfomycin (85.7%) and sulfamethoxazole + trimethoprim (80%) with IncI1. IncI1 appeared in 83.3%, 50%, and 100% of the isolates harboring blaCTX-M, blaTEM, and blaOXA β-lactamase genes, respectively.

Conclusions

The emergence of O26/IncI1/blaCTX-M STEC in cattle farms poses a potential risk to public health.
Feather meal processing methods impact the production parameters, blood biochemical indices, gut function, and hepatic enzyme activity in broilers
Authors: Safari Hassan , Mohit Ardeshir , Mohiti-Asli Maziar
Published: March 2024

This study investigated the effects of feather meal (FM) processing methods on production parameters, blood biochemical indices, intestinal morphology, digestive and hepatic enzyme activities, and gastrointestinal tract pH and microflora of broilers. A total of 480-d-old male broilers were used for 42 d in a completely randomized design with eight treatments and five replicates (12 chicks/replicate). Treatments were 1) a control diet (without FM), 2) a diet containing 4% raw FM (RFM), 3) a diet containing 4% processed FM (PFM) by autoclave (Au-PFM), 4) a diet containing 4% fermented FM (FFM) by Bacillus licheniformis (Bl-FFM), 5) a diet containing 4% FFM by Bacillus subtilis (Bs-FFM), 6) a diet containing 4% FFM by Aspergillus niger (An-FFM), 7) a diet containing 4% FFM by B. licheniformis + B. subtilis + A. niger (Co-FFM), and 8) a diet containing 4% PFM by an enzyme (En-PFM). Results showed that in the FFMs the contents of ash, ether extract, total volatile nitrogen, and amino acids including Lys, Met, Thr, Trp, His, Leu, Gly, Ile, Phe, and Tyr increased (P < 0.05), while crude fiber, crude protein, and dry matter content decreased (P < 0.05). Compared with the control, the Co-FFM diet had no significant differences (P > 0.05) in total body weight gain (2,827 vs. 2,791 g/chick), total feed intake (5,018 vs. 4,991 g/chick), European production efficiency factor (375 vs. 377), European Broiler Index (371 vs. 371), and feed conversion ratio (1.77 vs. 1.78 g/g). Feeding FFM decreased (P < 0.05) serum total cholesterol (1.46-fold), triglyceride (1.61-fold), very low-density lipoprotein cholesterol (1.61-fold), and low-density lipoprotein cholesterol (2.27-fold) compared to the control. Also, FFM increased (P < 0.05) villus height (1,045 to 1,351, 661 to 854, and 523 to 620 μm), and villus height to crypt depth ratio (6.15 to 8.45, 4.55 to 7.04, and 4.27 to 5.45), in the duodenum, jejunum, and ileum, respectively, compared to the control. Compared to the control, the Co-FFM diet increased (P < 0.05) protease (34, 39, and 45 %) in the pancreas, duodenum, and jejunum, as well as amylase (73, and 97 %) activities in the duodenum, and jejunum, respectively. Diets containing FFM reduced (P < 0.05) pH in the crop, gizzard, and ileum, and decreased (P < 0.05) Escherichia coli (6.12 to 5.70) count in ileum compared to the control. The Co-FFM diet increased (P < 0.05) lactic acid bacteria count in crop (6.77 to 7.50) and ileum (6.94 to 7.73), also decreased (P < 0.05) coliforms (6.31 to 5.75) count in ileum compared to the control. In conclusion, FM fermentation, particularly Co-FFM, improves the nutritional value of FM, converting it into a decent source of dietary protein for broilers.
Combining active learning and self-paced learning for cost-effective process design intents extraction of process data
Authors: Huang Rui , Zhu Shuyi , Huang Bo
Published: March 2024

With the widespread use of computer-aided technologies like CAD/CAM/CAPP in the product manufacturing process, a large amount of process data is constantly generated and data-driven process planning has shown promising potentials for effectively reusing the process knowledge. However, a lot of labeled data are needed to train a deep learning model for effectively extracting the embedded knowledge and experiences within these process data and the labeling of process data is quite expensive and time-consuming. This paper proposes a cost-effective process design intents extraction approach for process data by combining active learning (AL) and self-paced learning (SPL). First, the process design intents inference model based on Bi-LSTM is generated by using a few pre-labeled samples. Then, the prediction uncertainty of each unlabeled sample is calculated by using a Bayesian neural network, which can assist in the identification of high confidence samples in SPL and low confidence samples in AL. Finally, the low confidence samples with manual-labels and the high confidence samples with pseudo-labels are incorporated into the training data for retraining the process design intents inference model iteratively until the model attains optimal performance. The experiments demonstrate that our approach can substantially decrease the number of labeled samples required for model training and the design intents in the process data could be inferred effectively with dynamically undated training data.
Wavefield simulation of the acoustic VTI wave equation based on the adaptive-coefficient finite-difference frequency-domain method
Authors: Zhao Haixia , Wang Shaoru , Xu Wenhao
Published: March 2024

Many simulation methods have been developed for P-waves in vertically transversely isotropic (VTI) media. These methods are based on the acoustic approximation. The finite-difference frequency-domain (FDFD) method stands out for its ability to simulate multi-shot or narrowband seismic data. It has no temporal dispersion, facilitates attenuation modelling, and enables parallelization. The optimal FDFD method is commonly used to simulate the acoustic VTI wave equation, but it applies the same FDFD coefficients for different frequencies and model velocities, which cannot fully minimize the numerical dispersion error. To enhance its accuracy and effectiveness, we develop an adaptive-coefficient FDFD method specifically for the acoustic VTI wave equation. The FDFD coefficients depend on two factors: the number of wavelengths in each grid and the Thomsen parameters. The dispersion analysis reveals that the proposed FDFD method can achieve a reduction in the necessary number of grid points from 4 to 2.5 compared to the optimal nine-point average derivative method (ADM), while maintaining a maximum dispersion error of 1%. From three numerical examples, the developed FDFD method can obtain more accurate wavefield results than the ADM optimal FDFD method, while taking comparable computational time and memory.
Physical integrity and bioefficacy of used long-lasting insecticidal nets in Makenene, Centre Region of Cameroon
Authors: Djoufounna Joel , Takougan-Sonfouet Laeticia , Bamou Roland , Ngangue-Siewe Idriss N , Mbiakop Paulette N , Djiappi-Tchamen Borel , Tabue Raymond , Achu-Fosah Dorothy , Ateba Joel M , Mayi Marie P A , Awono-Ambene Parfait , Antonio-Nkondjio Christophe , Tchuinkam Timoléon
Published: March 2024

Long-lasting insecticide nets (LLINs) are the recommended tools against mosquito-borne diseases. However, their physical integrity and bioefficacy in the field could be affected by several factors. This study evaluated the physical integrity and bioefficacy of nets used in Makenene since 2016. Cross-sectional field surveys were carried out after 6 y. A questionnaire was first administered to the heads of households, and then the physical integrity of the LLINs was determined by calculating the proportional hole index (pHI). WHO cone bioassays were conducted to determine the bioefficacy of LLINs currently being used against wild strains of Anopheles gambiae s.l., Culex pipiens s.l., and laboratory-reared pyrethroid-susceptible strain of Anopheles coluzzii (Ngousso). Of the 167 LLINs examined in households, 39.5% were fairly good, 26.4% were acceptable, and 34.1% were damaged. The most torn faces of the nets were the sides used for entering and exiting. None of the 30 LLINs used for WHO cone bioassays was still effective against An. gambiae s.l. and Cx. pipiens s.l. while up to 85.7% of these LLINs were at least effective against the susceptible strain after 24 h, with a significant difference observed when comparing the mortality rates between wild and laboratory-susceptible strain of Anopheles (P-value < 0.01). Anopheles gambiae s.l. were all (100%) identified as An. gambiae s.s. by PCR. The LLINs distributed in Makenene since the 2016 campaign are only effective on susceptible strain and should be replaced for a better control of residual malaria transmission and the nuisance by Culex mosquitoes in the locality.
A case of conservatively managed idiopathic spinal cord herniation presenting with low-pressure headache
Authors: Mancuso-Marcello Marco , Frantzias Joseph , Hardwidge Carl
Published: March 2024

Idiopathic spinal cord herniation presenting with low-pressure headache is extremely rare. We present a case of thoracic ventral spinal cord herniation in a 35-year-old lady who presented with low-pressure headaches. To our knowledge, this is only the fourth case described in the literature of spontaneous ventral cord herniation presenting in this way. The patient was managed conservatively with no manifestation of focal neurological symptoms at 12-month follow-up. The proposed aetiology of spontaneous ventral cord herniation is an initial CSF leak via a dural defect, through which the cord subsequently also enters blocking the CSF leak. We endorse a conservative approach for patients who present similarly, secondary to the above pathophysiology.
Synchronous intussusception with primary neuroendocrine tumour in an adult
Authors: Turco Jennifer
Published: March 2024

Intussusception in adults is rare. There are only a few cases in the literature that report two lead points resulting in double or synchronous intussusception. We present a case of synchronous intussusception in a 45 year old man with neuroendocrine tumour of the ileocaecal valve and lipomatous polyp of the jejunum.
Simulations of galaxy cluster mergers with velocity-dependent, rare, and frequent self-interactions
Authors: Sabarish V M , Brüggen Marcus , Schmidt-Hoberg Kai , Fischer Moritz S , Kahlhoefer Felix
Published: March 2024

Self-interacting dark matter (SIDM) has been proposed to solve small-scale problems in $\rm {\Lambda CDM}$ cosmology. In previous work, constraints on the self-interaction cross-section of dark matter have been derived assuming that the self-interaction cross-section is independent of velocity. However, a velocity-dependent cross-section is more natural in most theories of SIDM. Using idealized N-body simulations without baryons, we study merging clusters with velocity-dependent SIDM. In addition to the usual rare scattering in the isotropic limit, we also simulate these systems with anisotropic, small-angle (frequent) scatterings. We find that the collisionless brightest cluster galaxy (BCG) has an offset from the DM peak that grows at later stages. Finally, we also extend the existing upper bounds on the velocity-independent, isotropic self-interaction cross-section to the parameter space of rare and frequent velocity-dependent self-interactions by studying the central densities of dark matter-only isolated haloes. For these upper-bound parameters, the DM-BCG offsets just after the first pericentre in the dark matter-only simulations are found to be ≲10 kpc. On the other hand, because of BCG oscillations, we speculate that the distribution of BCG offsets in a relaxed cluster is a statistically viable probe. Therefore, this motivates further studies of BCG off-centring in hydrodynamic cosmological simulations.
The vertical structure of galactic discs: non-local gravity versus dark matter
Authors: Kashfi Tahere , Roshan Mahmood
Published: March 2024

Recent isolated galactic simulations show that the morphology of galactic discs in modified gravity differs from that of the standard dark matter model. In this study, we focused on the vertical structure of galactic discs and compared the bending instability in the vertical direction for both paradigms. To achieve this, we utilized high-resolution N-body simulations to construct two models in a specific non-local gravity theory (NLG) and the standard dark matter model and compared their stability against the bending perturbations. Our numerical results demonstrate that the outer regions of the disc are more susceptible to the instability in NLG, whereas the disc embedded in the dark matter halo is more unstable in the central regions. We then interpret these results based on the dispersion relation of the bending waves. To do so, we presented an analytical study to derive the dispersion relation in NLG. Our numerical results align with the predictions of our analytical models. Consequently, we conclude that the analysis of bending instability in galactic discs offers an explanation for the distinct vertical structures observed in simulated galactic discs under these two theories. These findings represent a significant step towards distinguishing between the modified gravity and dark matter models.
Probing nuclear physics with supernova gravitational waves and machine learning
Authors: Mitra A , Orel D , Abylkairov Y S , Shukirgaliyev B , Abdikamalov E
Published: March 2024

Core-collapse supernovae (CCSNe) are sources of powerful gravitational waves (GWs). We assess the possibility of extracting information about the equation of state (EOS) of high density matter from the GW signal. We use the bounce and early post-bounce signals of rapidly rotating supernovae. A large set of GW signals is generated using general relativistic hydrodynamics simulations for various EOS models. The uncertainty in the electron capture rate is parametrized by generating signals for six different models. To classify EOSs based on the GW data, we train a convolutional neural network (CNN) model. Even with the uncertainty in the electron capture rates, we find that the CNN models can classify the EOSs with an average accuracy of about 87 per cent for a set of four distinct EOS models.
Tight stellar binaries favour active longitudes at sub- and antistellar points
Authors: Sethi Ritika , Martin David V
Published: March 2024

Stellar binaries are ubiquitous in the galaxy and a laboratory for astrophysical effects. We use TESS to study photometric modulations in the light curves of 162 unequal mass eclipsing binaries from the EBLM (Eclipsing Binary Low Mass) survey, comprising F/G/K primaries and M-dwarf secondaries. We detect modulations on 81 eclipsing binaries. We catalogue the rotation rates of the primary star in 69 binaries and discover 17 ellipsoidal variables. In a large portion (at least $\sim 51~{{\ \rm per\ cent}}$) of our sample, we detect photometric modulations consistent with two overdensities of spots on the primary star that are roughly 180° apart. We show that these so-called active longitudes are preferentially at the sub- and antistellar points on the primary star. Physically, this means that the spots on the primary star preferentially face directly towards and away from the secondary star.
Using Gaussian Processes to detect AGN flares
Authors: McLaughlin Summer A J , Mullaney James R , Littlefair Stuart P
Published: March 2024

A key feature of active galactic nuclei (AGN) is their variability across all wavelengths. Typically, AGN vary by a few tenths of a magnitude or more over periods lasting from hours to years. By contrast, extreme variability of AGN – large luminosity changes that are a significant departure from the baseline variability – are known as AGN flares. These events are rare and their time-scales poorly constrained, with most of the literature focusing on individual events. It has been suggested that extreme AGN variability including flares can provide insights into the accretion processes in the disc. With surveys such as the Legacy Survey of Space and Time promising millions of transient detections per night in the coming decade, there is a need for fast and efficient classification of AGN flares. The problem with the systematic detection of AGN flares is the requirement to detect them against a stochastically variable baseline; the ability to define a signal as a significant departure from the ever-present variability is a statistical challenge. Recently, Gaussian Processes have revolutionized the analysis of time-series data in many areas of astronomical research. They have, however, seen limited uptake within the field of transient detection and classification. Here, we investigate the efficacy of Gaussian Processes to detect AGN flares in both simulated and real optical light curves. We show that GP analysis can successfully detect AGN flares with a false-positive rate of less than seven per cent, and we present examples of AGN light curves that show extreme variability.
An implicit algorithm for simulating the dynamics of small dust grains with smoothed particle hydrodynamics
Authors: Elsender Daniel , Bate Matthew R
Published: March 2024

We present an implicit method for solving the diffusion equation for the evolution of the dust fraction in the terminal velocity approximation using dust-as-mixture smoothed particle hydrodynamics. The numerical scheme involves casting the dust diffusion equation into implicit form, rearranging into its resolvent cubic equation and solving analytically. This method is relevant for small grains that are tightly coupled to the gas, such as sub-micron dust grains in the interstellar medium or millimetre-sized dust grains in protoplanetary discs. The method avoids problems with the variable used to evolve the dust fraction becoming negative when evolved explicitly and is fast and accurate, avoiding the need for dust stopping time limiters and significantly reducing computational expense. Whilst this method is an improvement over using the explicit terminal velocity approximation method, as with any dust-as-mixture method it still fails to give accurate solutions in the limit of large (weakly coupled) grains.
Particle trapping and acceleration in turbulent post-flare coronal loops
Authors: Bacchini Fabio , Ruan Wenzhi , Keppens Rony
Published: March 2024

We present a study of energetic-electron trapping and acceleration in the Kelvin–Helmholtz-induced magnetohydrodynamic (MHD) turbulence of post-flare loops in the solar corona. Using the particle-tracing capabilities of MPI-AMRVAC 3.0, we evolve ensembles of test electrons (i.e. without feedback to the underlying MHD) inside the turbulent looptop, using the guiding-centre approximation. With the MHD looptop model of Ruan et al., we investigate the relation between turbulence and particle trapping inside the looptop structure, showing that better-developed turbulent cascades result in more efficient trapping primarily due to mirror effects. We then quantify the electron acceleration in the time-evolving MHD turbulence, and find that ideal-MHD processes inside the looptop can produce non-thermal particle spectra from an initial Maxwellian distribution. Electrons in this turbulence are preferentially accelerated by mirror effects in the direction perpendicular to the local magnetic field while remaining confined within small regions of space between magnetic islands. Assuming dominance of Bremsstrahlung radiation mechanisms, we employ the resulting information from accelerated electrons (combined with the MHD background) to construct HXR spectra of the post-flare loop that include non-thermal-particle contributions. Our results pave the way to constructing more realistic simulations of radiative coronal structure for comparison with current and future observations.
Observations of spiral and streamer on a candidate proto-brown dwarf
Authors: Riaz B , Stamatellos D , Machida M N
Published: March 2024

Spirals and streamers are the hallmarks of mass accretion during the early stages of star formation. We present the first observations of a large-scale spiral and a streamer towards a very young brown dwarf candidate in its early formation stages. These observations show, for the first time, the influence of external environment that results in asymmetric mass accretion via feeding filaments on to a candidate proto-brown dwarf in the making. The impact of the streamer has produced emission in warm carbon-chain species close to the candidate proto-brown dwarf. Two contrasting scenarios, a pseudo-disc twisted by core rotation and the collision of dense cores, can both explain these structures. The former argues for the presence of a strong magnetic field in brown dwarf formation while the latter suggests that a minimal magnetic field allows large-scale spirals and clumps to form far from the candidate proto-brown dwarf.
Heavy Ion Radiolysis of the Chiral Terpene α-pinene
Authors: de Barros A L F , Ricca A , Bychkova A , da Costa C A P , Costa J W , Boduch P , Rothard H , da Silveira E F , Domaracka A
Published: March 2024

Radiolysis of α-pinene by 61.3 MeV 84Kr15 + ions was analysed with the scope to simulate the effects of heavy ion cosmic ray bombardment on chiral molecules in the interstellar medium. The α-pinene ice samples were irradiated at 10 K and their chemical evolution was monitored by mid-infrared Fourier transform (FTIR) spectroscopy to characterize the reaction products and to determine the extent of racemization. The integrated band strengths have been obtained for all the neutral α-pinene vibrational bands using the experimental band integrated absorbances and the theoretical absolute intensities calculated along the column densities. In the current heavy ion bombardment experiments, small molecules were formed and the precursor, α-pinene, was destroyed instead of being racemized. Twelve hydrocarbons were produced (final fluence of 2.0 × 1012 ions cm−2): methane (CH4), acetylene (C2H2), ethylene (C2H4), propylene (C3H6), propane (C3H8), n-butane (C4H10), butene (C4H8), propyne (C3H4), benzene (C6H6), ethane (C2H6), vinylacetylene (C4H4), and 2-methyl-1,3-butadiene or isoprene (C5H8). The highest formation cross-section (∼ 40 × 10−15 cm2) was observed for the C3H4 and the lowest was for C3H8 (∼ 3 × 10−15 cm2). The radiochemical yields for these molecules follow the same trends as those of their cross-sections. The atom budget calculation confirms that all the expected products have been generated during the radiolysis and supports the conclusion that the proposed A values are accurate. The α-pinene sputtering yield for this ion beam was found to be Y0  = 1.84 × 106 molecules per impact.
CRISPR antiphage defence mediated by the cyclic nucleotide-binding membrane protein Csx23
Authors: Grüschow Sabine , McQuarrie Stuart , Ackermann Katrin , McMahon Stephen , Bode Bela E , Gloster Tracey M , White Malcolm F
Published: March 2024

CRISPR-Cas provides adaptive immunity in prokaryotes. Type III CRISPR systems detect invading RNA and activate the catalytic Cas10 subunit, which generates a range of nucleotide second messengers to signal infection. These molecules bind and activate a diverse range of effector proteins that provide immunity by degrading viral components and/or by disturbing key aspects of cellular metabolism to slow down viral replication. Here, we focus on the uncharacterised effector Csx23, which is widespread in Vibrio cholerae. Csx23 provides immunity against plasmids and phage when expressed in Escherichia coli along with its cognate type III CRISPR system. The Csx23 protein localises in the membrane using an N-terminal transmembrane α-helical domain and has a cytoplasmic C-terminal domain that binds cyclic tetra-adenylate (cA4), activating its defence function. Structural studies reveal a tetrameric structure with a novel fold that binds cA4 specifically. Using pulse EPR, we demonstrate that cA4 binding to the cytoplasmic domain of Csx23 results in a major perturbation of the transmembrane domain, consistent with the opening of a pore and/or disruption of membrane integrity. This work reveals a new class of cyclic nucleotide binding protein and provides key mechanistic detail on a membrane-associated CRISPR effector.
Structural change, labour reallocation, and productivity growth in post-reform China
Authors: Li Wenliang
Published: March 2024

Why has the structural bonus been so small during China’s post-reform era? This article explains the puzzle by exploiting the heterogeneity in structural-change patterns across Chinese provinces. Using an original database covering production and factor inputs in 8 sectors and 31 provinces over 1993–2016, I show only those provinces where labour was reallocated from agriculture to manufacturing and services benefited from a structural bonus on labour productivity growth. In the other provinces, this structural-change effect was minimal or negative. Regarding the structural-change effect on total factor productivity (TFP) growth, I find both labour and capital reallocation played a limited role. Labour reallocation has little potential in boosting aggregate TFP growth, as marginal labour returns are similar across sectors. Capital reallocation has a far greater potential but remains restrained, suggesting substantial reallocation frictions. China’s TFP growth is mostly explained by within-sector technological progress, which has been dissipating since 2008, leading to declining TFP growth.
Remarks on Effects of Projective Phase on Eigenstate Thermalization Hypothesis
Authors: Fukushima Osamu
Published: March 2024

The existence of p-form symmetry in a (d + 1)-dimensional quantum field is known to always lead to the breakdown of the eigenstate thermalization hypothesis for certain (d − p)-dimensional operators other than symmetry operators under some assumptions. The assumptions include the mixing of symmetry sectors within a given energy shell, which is rather challenging to verify because it requires information on the eigenstates in the middle of the spectrum. We reconsider this assumption from the viewpoint of projective representations to avoid this difficulty. In the case of $\mathbb {Z}_N$ symmetries, we can circumvent the difficulty by considering $\mathbb {Z}_N\times \mathbb {Z}_N$-symmetric theories with nontrivial projective phases, and perturbing the Hamiltonian while preserving one of the $\mathbb {Z}_N$ symmetries of our interest. We also perform numerical analyses for (1 + 1)-dimensional spin chains and the (2 + 1)-dimensional $\mathbb {Z}_2$ lattice gauge theory.
Preslaughter factors affecting mobility, blood parameters, bruising, and muscle pH of finished beef cattle in the United States
Authors: Sullivan Paxton A , Davis Melissa K , Nair Mahesh N , Hess Ann M , Mooney Daniel F , Edwards-Callaway Lily N
Published: March 2024

Decades of work have focused on reducing fear, stress, and discomfort in cattle during the preslaughter phase by improving and promoting animal handling, transportation, and management processes. Even still, there is limited information about the effects of preslaughter factors on animal welfare and meat quality outcomes in finished cattle in the United States. This study aimed to track individual animals through the slaughter process to identify preslaughter factors associated with key welfare and quality outcomes. A total of 454 cattle from one commercial slaughter facility were studied. Preslaughter factors assessed included distance traveled, truck waiting time, lairage density, lairage duration, and season. Animal characteristics, i.e., body weight, breed, and sex, were also recorded. One trained observer scored the mobility of all cattle using the North American Meat Institute’s 1-4 scale (i.e., normal to extremely reluctant to move). Exsanguination blood was collected and analyzed for cortisol, creatine kinase, and lactate. Carcass bruising was scored using a modified version of the National Beef Quality Audit’s bruise-scoring methodology (i.e., no bruise, one bruise ≤ the size of a deck of cards, one bruise > than the size of a deck of cards, and multiple bruises). Ultimate muscle pH was measured 32 to 36 h postmortem. Multi-predictor models were selected for each outcome variable using Akaike Information Criterion. Continuous outcome variables were analyzed using linear mixed-effect models and categorical outcome variables with mixed-effect logistic regression models. Longer truck waiting times were associated with increased cortisol (P = 0.04) and lactate (P = 0.02) concentrations. Similarly, increased lairage duration was associated with increased creatine kinase concentrations (P = 0.05) and the odds of cattle being bruised (P = 0.03). Less space allowance per animal in lairage was associated with increased odds of cattle having impaired mobility (P = 0.01). There was a seasonal effect for many of the measured outcomes; the summer season was associated with greater lactate concentrations (P < 0.0001), increased odds of impaired mobility (P < 0.0001), and increased odds of carcass bruising (P = 0.003). The findings of this study indicate that many of the preslaughter factors assessed influence critical welfare and meat quality outcomes of finished beef cattle, warranting future research and consideration.
Financial market regulation and private law: a new frontier of transnational commercial law
Authors: Wittmann Josef
Published: March 2024

Global financial markets are among the most extensively regulated markets in the world economy. This poses significant challenges from a private law perspective when regulatory rules interfere with private rights and obligations. This article examines the potential of transnational commercial law (TCL) to provide for a private law framework suited to the regulatory reality in global financial markets. The article finds that, while regulatory aspects are generally excluded from TCL instruments, notable exceptions to this general rule occur. Based on an analysis of the approaches undertaken in the Unidroit Convention of Substantive Rules for Intermediated Securities (Geneva Convention), including the accompanying Legislative Guide, the Unidroit Principles on the Operation of Close-Out Netting Provisions and the UNCITRAL Model Law on Secured Transactions, the article maps out three methods that allow for a reconciliation of private and regulatory rules: (i) the adoption of a flexible private law system including default and conflict rules; (ii) the use of opening clauses to allow deviations from TCL rules in accordance with international regulatory standards; and (iii) a codificatory approach through the development of a comprehensive and systematic legal framework consisting of both private law and regulatory rules. Finally, the article addresses potential obstacles and objections that future TCL instruments will face when addressing regulatory aspects. Overall, the article argues that an efficient financial market requires a foreseeable set of private law rules that—considering the continuous expansion of regulatory law—must address the pressing issues deriving from the interrelation of regulation and private law. However, the adaption of TCL instruments should be limited to common-sense financial market regulation that, even if differing from State to State, generally follows globally shared regulatory goals.
Multigene phylogeny and expanded morphological comparison reveal one new lineage and finer differentiation in the order Peniculida (Alveolata: Ciliophora)
Authors: Wang Tian , Lu Linger , Sun Cuiming , Li Tao , Zhao Yan , Ni Bing , Fan Xinpeng
Published: March 2024

Frontoniidae, the largest family of the order Peniculida, is non-monophyletic with its members dispersed among other families in phylogenetic trees, which complicated the unsolved inner relationship of the order. In this study, we conducted phylogenetic analyses using 34 newly obtained nuclear sequences and 29 COI sequences from the families Frontoniidae and Stokesiidae. Additionally, the nematodesmata of Frontoniidae species were described using light and electron microscopy. Our findings revealed that Frontonia depressa represents a distinct lineage within the order Peniculida, leading to the establishment of a new genus, Protofrontonia, based on the distinctive morphology of F. depressa as a diagnostic feature. The finer differentiation of nematodesmata and cysts were adopted as novel characters for evolutionary discussion of the major Peniculida lineages. We propose that the strengthened nematodesmata, enabling the ability to feed on larger food, represents a derived feature of the ‘true’ Frontoniidae lineage, while a cyst with a papula may serve as a common derived feature of Stokesiidae and its Frontoniidae affinity. It is suggested that divergent evolution may have also occurred in the lineages retaining Frontoniidae plesiomorphies, albeit not reflected in the traditional taxonomic features.