bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Structure and Historical Dynamics of Pinus densiflora Siebold & Zucc. Populations
Authors: Jia Zhiyuan , Yu Tao , Jiang Bin , Song Xin , Li Junqing
Published: December 2022

The study of population genetic structure and historical dynamics of species with disjunct distribution can reveal the mechanisms through with they were formed. Pinus densiflora is an essential tree species with ecological and economic value, and its natural distribution shows a disjunct pattern. Using transcriptome-level SNP data from 220 samples representing 32 naturally-distributed populations in East Asia, we investigated Pinus densiflora genetic diversity and structure, divergence time, and ancestral distribution. We identified five subpopulations which diverged approximately 2.02–1.49 million years ago, and found relatively low genetic differentiation among the three large subpopulations (SL, JH, and JK). Northeast China is the most likely origin, and its current distribution is the result of dispersal and vicariance events. It migrated southwest through the Liaodong Peninsula to the Shandong Peninsula and southeast through the Korean Peninsula to Japan. These results provide a basis for the conservation and management of P. densiflora in the future and the evolutionary study of species with similar life histories.
Carbonaceous Materials from Forest Waste Conversion and Their Corresponding Hazardous Pollutants Remediation Performance
Authors: Ungureanu Gabriela , Bejenari Iuliana , Hristea Gabriela , Volf Irina
Published: December 2022

The conversion of lignocellulosic waste ascends as a promising path to generate new materials with wide industrial and environmental uses. Pyrolytic biochar (PBc), hydrochar (Hc), and activated hydrochar (AcHc) produced from the waste of Picea Abies bark were considered bio-based sorbents for lead uptake from aqueous effluents. PBc was obtained through slow pyrolysis (550 °C), while Hc resulted under hydrothermal conversion (280 °C). In order to enhance the specific surface, Hc was subjected to a physical activation, resulting AcHc. All three carbonaceous materials were prepared through relatively simple processes from a readily locally available resource. The carbonaceous materials were characterized using infrared spectroscopy and scanning electron microscopy. The Pb (II) removal has been tested in batch mode on a synthetic monocomponent wastewater matrix, as well as on a real mine drainage effluent. A significant effect of pH was observed, while the equilibrium was achieved in a short time, about 60 min for PBc and Hc and 120 min for AcHc. Langmuir model predicted a maximum adsorption capacity of 15.94 mg/g for PBc, 9.99 mg/g for Hc, and 37.46 mg/g for AcHc. All materials studied had good uptake capacities for lead with no drastic effect of typical coexisting species.
Mechanical Properties of Three Bamboo Species: Effect of External Climatic Conditions and Fungal Infestation in Laboratory Conditions
Authors: Elejoste Asier , Osa Juan Luis , Arevalillo-Roman Alfonso , Eceiza Arantxa , Abascal Jose Miguel , Rico-Martinez Jose Miguel , Butron Amaia , Peña-Rodriguez Cristina
Published: December 2022

Bamboo is a material with good tensile and flexural resistance. As a construction material with structural capacity, using bamboo implies considerable environmental advantages in relation to other typical materials such as steel or concrete. For its correct implementation, it is necessary to define its mechanical properties and durability. Bamboo is susceptible to degradation due to the lack of natural toxins and thin walls, which means that shallow decomposition processes can imply appreciable reductions in its mechanical capacity. The main degrading agents considered in this study were beetles, termites, and xylophagous fungi. The aim of this study was to analyze the durability of three different species: DS, PA, and AA. Durability and mechanical tests results after 6 months of exposure to biotic and abiotic agents were compared with their original properties and chemical composition. In this study, durability was analyzed in two ways. Firstly, the loss of mass due to fungal infection was investigated. The results obtained were based on the standard EN 113 using the fungus CP. Secondly, bending and compressive strength was evaluated after a durability test according to the standard EN 335:2013 for the CU3.1 use class after a 6 month period in the city of Donostia/San Sebastian, Spain. The DS and AA varieties were rated as very durable CD1, while the PA variety is durable CD2, thus proving to be an attractive material for construction.
A Low-Cost Open-Source Architecture for a Digital Signage Emergency Evacuation System for Cruise Ships, Based on IoT and LTE/4G Technologies
Authors: Cheimaras Vasileios , Trigkas Athanasios , Papageorgas Panagiotis , Piromalis Dimitrios , Sofianopoulos Emmanouil
Published: December 2022

During a ship evacuation, many people panic as they do not know the direction that leads to the emergency muster station. Moreover, sometimes passengers get crowded in corridors or stairs, so they cannot save their lives. This paper proposes an IoT-enabled architecture for digital signage systems that directs passengers to the muster stations of a cruise ship by following the less dangerous route. Thus, crews’ and passengers’ safety risks during a ship evacuation can be low, and human health hazards may be limited. The system is based on a low-cost and open-source architecture that can also be used in a variety of fields in industrial IoT applications. The proposed modular digital signage architecture utilizes Light Emitting Diode (LED) strips that are remotely managed through a private Long-Term Evolution (LTE)/Fourth Generation (4G) cellular network. Publish–subscribe communication protocols were used for the control of the digital strips and particularly through a Message Queuing Telemetry Transport (MQTT) broker who publishes/subscribes every message to specific topics of the realized IoT platform, while the overall digital signage system centralization was implemented with an appropriate dashboard supported from an open-source RESTful API.
Adjustment of the Grass Fuel Moisture Code for Grasslands in Southern Brazil
Authors: Labres dos Santos João Francisco , Kovalsyki Bruna , Ferreira Tiago de Souza , Batista Antonio Carlos , Tetto Alexandre França
Published: December 2022

Grasslands are one of the vegetation types most widely affected by wildfires in southern Brazil. It is a fire-dependent ecosystem and it is necessary to know the hourly fuel moisture variation for its management. The objective of this work is to fit Grass Fuel Moisture Code (GFMC) models to estimate the moisture content for the grassland of the State Park of Vila Velha, Paraná, Brazil. Data sampling to determine the hourly moisture content was performed during the winter of 2018 and divided into two campaigns of five days with stable weather conditions. Destructive samples were taken out for the sorption tests on climatic chambers to obtain the equilibrium moisture content and the time lag values. The fitted equilibrium moisture and time lag models were evaluated by residual distribution analysis, mean absolute error (MAE), root mean square error (RSME) and coefficient of determination (R2). The fitted model performed better than the original GFMC model due to the obtained MAE, RSME and R2 values. The results showed that the fitted GFMC model is better to predict the fine fuel moisture for the region.
Erythrocyte Respiratory Activity of the Mitochondrial Complex of the Black Sea Thornback Ray (Raja clavata L.) under the Influence of Certain Activators and Inhibitors In Vitro
Authors: Silkin Yuriy A. , Silkin Mikhail Yu. , Korotkov Sergey M. , Silkina Elizaveta N. , Silkina Alla
Published: December 2022

Global warming implies the risk of a changing oxygen regime in the seas and oceans of our planet. The mitochondrial complex of nuclear erythrocytes of cartilaginous fish, as the energy basis of blood cells, has repeatedly encountered such climatic fluctuations throughout their evolutionary history. In this regard, the features of the adaptive strategy of the erythrocyte mitochondrial complex in the thornback ray (Raja clavata L.) are of interest from the evolutionary and ecological points of view. The rate of oxygen consumption in resuspended (Ht = 25–30%) erythrocytes taken from the Black Sea thornback ray in saline was studied by the polarographic method. A high “basal” rate of respiration in the erythrocytes of the thornback ray was shown, which ranged from 10.5 to 21.6 pmol O2 min−1·106 cells. The addition of substrates of the mitochondrial respiration activators glutamate, maleate, and succinate to the erythrocyte suspension caused a 2–6-fold increase in the respiratory activity of thornback ray erythrocytes. In cases where the rate of respiration of erythrocytes was high, protonophore–dinitrophenol caused an inhibition of the activity of mitochondrial respiration. At low respiration rates of erythrocytes, its effect was opposite and caused a stimulation of mitochondrial respiration. Oligomycin caused a significant inhibition of the respiratory activity of the red blood cell suspension of the thornback ray. This suppression of cell respiration was enhanced under conditions of exposure to the permeabilization of erythrocytes with digitonin. This can be recommended as one of the ways to block the respiratory activity of erythrocytes in cartilaginous fish. Another way of effectively blocking the respiration of the mitochondrial complex of the thornback ray’s erythrocytes was the effect of the blockers rotenone and sodium azide. The peculiarity of the mitochondria of the erythrocytes of the thornback ray was the absence of the complete inhibition of respiration by sodium azide (NaN3), which is characteristic of the mitochondria of other fish species. Our data on the activation of the “respiration” of erythrocytes in fish indicate that the potential capabilities of cold-blooded and warm-blooded vertebrates have rather similar characteristics. This may indicate the initial “laying” of the architecture of the inner membrane to support the energy potential of the mitochondria of the cell.
Injectable Composite Systems of Gellan Gum:Alginate Microparticles in Pluronic Hydrogels for Bioactive Cargo Controlled Delivery: Optimization of Hydrogel Composition based on Rheological Behavior
Authors: Carrêlo Henrique , Escoval André R. , Soares Paula I. P. , Borges João P. , Cidade Maria Teresa
Published: December 2022

Due to the high complexity of some treatments, there is a need to develop drug-delivery systems that can release multiple drugs/bioactive agents at different stages of treatment. In this study, a thermoresponsive injectable dual-release system was developed with gellan gum/alginate microparticles (GG:Alg) within a thermoresponsive Pluronic hydrogel composed of a mixture of Pluronic F127 and F68. The increase in F68 ratio and decrease in F127 lead to higher transition temperatures. The addition of the GG:Alg microparticles decreased the transition temperatures with a linear tendency. In Pluronic aqueous solutions (20 wt.%), the F127:F68 ratios of 16:4 and 17:3 (wt.%:wt.%) and the addition of microparticles (up to 15 wt.%) maintained the sol–gel transition temperatures within a suitable range (between 25 °C and 37 °C). Microparticles did not hinder the injectability of the system in the sol phase. Methylene blue was used as a model drug to evaluate the release mechanisms from microparticles, hydrogel, and composite system. The hydrogel delayed the release of methylene blue from the microparticles. The hydrogel loaded with methylene blue released at a faster rate than the microparticles within the hydrogel, thus demonstrating a dual-release profile.
The Effects of Soluble Dietary Fibers on Glycemic Response: An Overview and Futures Perspectives
Authors: Giuntini Eliana Bistriche , Sardá Fabiana Andrea Hoffmann , de Menezes Elizabete Wenzel
Published: December 2022

The properties of each food, composition, and structure affect the digestion and absorption of nutrients. Dietary fiber (DF), especially viscous DF, can contribute to a reduction in the glycemic response resulting from the consumption of carbohydrate-rich foods. Target and control of postprandial glycemic values are critical for diabetes prevention and management. Some mechanisms have been described for soluble DF action, from the increase in chyme viscosity to the production of short-chain fatty acids resulting from fermentation, which stimulates gastrointestinal motility and the release of GLP-1 and PYY hormones. The postprandial glycemic response due to inulin and resistant starch ingestion is well established. However, other soluble dietary fibers (SDF) can also contribute to glycemic control, such as gums, β-glucan, psyllium, arabinoxylan, soluble corn fiber, resistant maltodextrin, glucomannan, and edible fungi, which can be added alone or together in different products, such as bread, beverages, soups, biscuits, and others. However, there are technological challenges to be overcome, despite the benefits provided by the SDF, as it is necessary to consider the palatability and maintenance of their proprieties during production processes. Studies that evaluate the effect of full meals with enriched SDF on postprandial glycemic responses should be encouraged, as this would contribute to the recommendation of viable dietary options and sustainable health goals.
A New Approach in Meat Bio-Preservation through the Incorporation of a Heteropolysaccharide Isolated from Lobularia maritima L.
Authors: Akacha Boutheina Ben , Najar Basma , Venturi Francesca , Quartacci Mike Frank , Saad Rania Ben , Brini Faiçal , Mnif Wissem , Kačániová Miroslava , Ben Hsouna Anis
Published: December 2022

In this study, a new heteropolysaccharide extracted from Lobularia maritima (L.) Desv. (LmPS), a halophyte harvested in Tunisia, was evaluated as an antioxidant and antibacterial additive in the bio-preservation of raw minced meat. For antibacterial testing, Gram-positive bacteria such as Staphylococcus aureus ATCC and Listeria monocytogenes ATCC 19,117 and Gram-negative bacteria such as Salmonella enterica ATCC 43,972 and Escherichia coli ATCC 25,922 were used. The results indicate that this polymer had a significant antibacterial activity against foodborne pathogens. Additionally, the effects of LmPS at 0.15, 0.3 and 0.6% on refrigerated raw ground beef were investigated from a microbiological, chemical, and sensory perspective. Microbiological analysis of the meat showed that treatment with LmPS significantly (p < 0.05) improved its shelf life, while the biochemical analysis evidenced a significant (p < 0.05) decrease in lipid oxidation. LmPS at 0.6% significantly reduced by 61% and 48% metmyoglobin accumulation at the end of the storage period when compared to BHT and control samples, respectively. The chemometric approach highlighted the relationships among the different meat quality parameters. LmPS can be introduced in the food industry as a powerful natural additive and could be an alternative to synthetic antioxidant compounds.
Gastrointestinal Digestion and Microbial Hydrolysis of Alkyl Gallates: Potential Sustained Release of Gallic Acid
Authors: Wang Xinmiao , Wang Qian , Hu Yuanyuan , Yin Fawen , Liu Xiaoyang , Zhou Dayong
Published: December 2022

Phenolipids such as alkyl gallates (A-GAs) have been approved by the food industry as non-toxic antioxidant additives, which are also regarded as an emerging source of functional food ingredients. However, comprehensive understanding of their digestive absorption is needed. Thus, the models of live mice and anaerobic fermentation were used to clarify the distribution and microbial hydrolysis characteristics of A-GAs in the gastrointestinal tract. HPLC-UV results demonstrated that A-GAs could be hydrolyzed by intestinal lipases and gut microorganisms including Lactobacillus to produce free gallic acid (GA). Through regulating the chain length of the lipid part in A-GAs, the sustained and controllable release of the GA can be easily achieved. Furthermore, A-GAs were also able to reach the colon and the cecum, which would lead to potential gastrointestinal protective effects. Therefore, A-GAs may be applied as possible ingredient for functional foods.
Non-Conventional Induction Heat Treatment: Effect of Design and Electrical Parameters on Apple Juice Safety and Quality
Authors: Wu Shilin , Xu Xueming , Yang Na , Jin Yamei , Jin Zhengyu , Xie Zhengjun
Published: December 2022

The proposed non-conventional induction heating, which combines an MSCP and VDC structure, was proved to have excellent thermal effect. Different from other electric field sterilization, this electrotechnology operates with no electrodes, and it is a continuous-flow process with short-duration (about 20 s). In current study, the parameters related to temperature rise were investigated, including applied voltage, frequency, the diameter of the secondary coil and heating tube, as well as their length, etc. It was demonstrated that a smaller diameter of the heating tube, parallel connection sample coils, and higher frequency were beneficial for the inactivation of microorganisms. At 500 Hz, the optimal condition is 800 V, d1 = 2 mm, and L1 = 10 cm. Notably, the system could inactivate all microorganisms and maintained the physicochemical properties of apple juice at 40 kHz. It suggests that this structural design has the potential for industrial applications and the proposed induction heating can realize the rapid sterilization of liquid food without applying electrodes.
A Review of the Effects of Puerarin on Glucose and Lipid Metabolism in Metabolic Syndrome: Mechanisms and Opportunities
Authors: Jing Xiaoxuan , Zhou Jingxuan , Zhang Nanhai , Zhao Liang , Wang Shiran , Zhang Liebing , Zhou Feng
Published: December 2022

Chronic diseases, including metabolic syndrome related to sugar and lipid metabolic disorders, are the leading causes of premature death around the world. Novel treatment strategies without undesirable effects are urgently needed. As a natural functional ingredient, puerarin is a promising alternative for the treatment of sugar and lipid metabolic disorders. However, the applications of puerarin are limited due to its poor solubility and short half-life. Various drug delivery systems have been investigated to improve the bioavailability of puerarin. This review summarizes the mechanisms involved in the beneficial action of puerarin: suppressing the release of glucose and FFA; regulating the transport of glucose and fatty acids; acting on the PI3K–Akt and AMPK signaling pathways to decrease the synthesis of glucose and fatty acids; acting on the PPAR signaling pathway to promote β-oxidation; and improving insulin secretion and sensitivity. In addition, the preparation technologies used to improve the bioavailability of puerarin are also summarized in this review, in the hope of helping to promote the application of puerarin.
Quality Aspects of Designing Prohealth Liver Sausages Enriched with Walnut Paste
Authors: Florowski Tomasz , Florowska Anna , Chmiel Marta , Adamczak Lech , Pietrzak Dorota , Ostrowska Agnieszka , Szymańska Iwona
Published: December 2022

The aim of the study was to determine the influence of enriching liver sausages with different levels of walnut paste on the quality properties of this product. Sausages were produced with 5, 10, 15, 20, and 25% amount additions of walnut paste and without the addition of nuts (control product). It was found that walnut paste, especially when introduced at an amount >15%, was a component that limited thermal losses and significantly modified the characteristics of liver sausages. The addition of walnut paste also increased the fat content of liver sausages by two–three times, which was one of the factors that weakened their structure, including lowering their compression, shear, and penetration force but increasing their spreadability. Moreover, the addition of walnut paste at an amount of ≥20% resulted in the products having a slightly different color, with lower values for the a* color parameter. Such changes were assessed as a favorable modification to the product, increasing its overall desirability, especially with the addition of walnut paste at the level of 20%. Walnut paste can therefore be a valuable ingredient that allows for the development of a health-promoting product with improved quality features. However, with the addition of a walnut paste at an amount of 25%, it is necessary to take into account the more rapid and unfavorable fat changes that occur during the storage of the liver sausages, as indicated by about 50% higher TBARS values (compared to the control product).
Obtention of Sacha Inchi (Plukenetia volubilis Linneo) Seed Oil Microcapsules as a Strategy for the Valorization of Amazonian Fruits: Physicochemical, Morphological, and Controlled Release Characterization
Authors: Rodríguez-Cortina Aureliano , Rodríguez-Cortina Jader , Hernández-Carrión María
Published: December 2022

Sacha inchi seed oil (SIO) is a promising ingredient for the development of functional foods due to its large amount of high-value compounds; however, it is prone to oxidation. This work aimed to obtain SIO microcapsules using conventional and ultrasound probe homogenization and using spray- and freeze-drying technologies as effective approaches to improve the long-term stability of functional compounds. The application of ultrasound probe homogenization improved the rheological and emulsifying properties and decreased the droplet size and interfacial tension of emulsions. The microcapsules obtained by both drying technologies had low moisture (1.64–1.76) and water activity (0.03–0.11) values. Spray-dried microcapsules showed higher encapsulation efficiency (69.90–70.18%) compared to freeze-dried ones (60.02–60.16%). Thermogravimetric analysis indicated that heat protection was assured, enhancing the shelf-life. Results suggest that both drying technologies are considered effective tools to produce stable microcapsules. However, spray-drying technology is positioned as a more economical alternative to freeze-drying.
Effect of Modified Manufacturing Conditions on the Composition of Greek Strained Yogurt and the Quantity and Composition of Generated Acid Whey
Authors: Karastamatis Stavros , Zoidou Evangelia , Moatsou Golfo , Moschopoulou Ekaterini
Published: December 2022

Greek strained yogurt is produced in high quantities worldwide. This production leaves behind acid whey, a by-product that is an environmental challenge. Hence, efforts are made to minimize the acid whey generation. In this study, the combined effect of the different heat treatment levels of milk and the different time of straining on the composition of the produced strained yogurt, as well as on the quantity and composition of the expelled acid whey, was investigated. The initial yogurts were prepared with bovine milk heated at 85 °C/16 s or 100 °C/16 s or 90 °C/5 min, and the acid whey was removed by centrifugation (5500 rpm, 5 min, 25 °C) either immediately after incubation or after 24 h. The results showed that, regardless of the heat treatment of milk, straining after 24 h resulted in an 8% increase in the yield in strained yogurt and about an 11% decrease in the generated acid whey, compared to straining immediately after incubation. The heat treatment level of milk significantly influenced the fat, lactose, and total solids contents of the strained yogurts, as well as the residual whey proteins, protein, and total solids contents of acid whey. Yogurt’s sensory properties were not affected significantly. It was concluded that the quantity of the acid whey expelled during the production of Greek strained yogurt could be decreased without affecting the general quality of the yogurt.
Fractal Curves on Banach Algebras
Authors: Navascués María A.
Published: December 2022

Most of the fractal functions studied so far run through numerical values. Usually they are supported on sets of real numbers or in a complex field. This paper is devoted to the construction of fractal curves with values in abstract settings such as Banach spaces and algebras, with minimal conditions and structures, transcending in this way the numerical underlying scenario. This is performed via fixed point of an operator defined on a b-metric space of Banach-valued functions with domain on a real interval. The sets of images may provide uniparametric fractal collections of measures, operators or matrices, for instance. The defining operator is linked to a collection of maps (or iterated function system, and the conditions on these mappings determine the properties of the fractal function. In particular, it is possible to define continuous curves and fractal functions belonging to Bochner spaces of Banach-valued integrable functions. As residual result, we prove the existence of fractal functions coming from non-contractive operators as well. We provide new constructions of bases for Banach-valued maps, with a particular mention of spanning systems of functions valued on C*-algebras.
Ultrasonic Treatment of Food Colloidal Systems Containing Oleogels: A Review
Authors: Frolova Yuliya , Sarkisyan Varuzhan , Sobolev Roman , Kochetkova Alla
Published: December 2022

The use of oleogels as an alternative to solid fats to reduce the content of saturated and trans-isomeric fatty acids is a developing area of research. Studies devoted to the search for methods of obtaining oleogels with given properties are of current interest. Ultrasonic treatment as a method for modifying oleogel properties has been used to solve this problem. The number of publications on the study of the effect of ultrasonic treatment on oleogel properties is increasing. This review aimed to systematize and summarize existing data. It allowed us to identify the incompleteness of this data, assess the effect of ultrasonic treatment on oleogel properties, which depends on various factors, and identify the vector of this direction in the food industry. A more detailed description of the parameters of ultrasonic treatment is needed to compare the results between various publications. Ultrasonic treatment generally leads to a decrease in crystal size and an increase in oil-binding capacity, rheological properties, and hardness. The chemical composition of oleogels and the concentration of gelators, the amplitude and duration of sonication, the cooling rate, and the crystallization process stage at which the treatment occurs are shown to be the factors influencing the efficiency of the ultrasonic treatment.
Identification of an Amino Acid Metabolism-Related Gene Signature for Predicting Prognosis in Lung Adenocarcinoma
Authors: Chang Wuguang , Li Hongmu , Wu Chun , Zhong Leqi , Zhu Tengfei , Chang Zenghao , Ou Wei , Wang Siyu
Published: December 2022

Dysregulation of amino acid metabolism (AAM) is an important factor in cancer progression. This study intended to study the prognostic value of AAM-related genes in lung adenocarcinoma (LUAD). Methods: The mRNA expression profiles of LUAD datasets from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were applied as the training and validation sets. After identifying the differentially expressed AAM-related genes, an AAM-related gene signature (AAMRGS) was constructed and validated. Additionally, we systematically analyzed the differences in immune cell infiltration, biological pathways, immunotherapy response, and drug sensitivity between the two AAMRGS subgroups. Results: The prognosis-related signature was constructed on the grounds of key AAM-related genes. LUAD patients were divided into AAMRGS-high and -low groups. Patients in the two subgroups differed in prognosis, tumor microenvironment (TME), biological pathways, and sensitivity to chemotherapy and immunotherapy. The area under the receiver operating characteristics (ROC) and calibration curves showed good predictive ability for the nomogram. Analysis of immune cell infiltration revealed that the TME of the AAMRGS-low group was in a state of immune activation. Conclusion: We constructed an AAMRGS that could effectively predict prognosis and guide treatment strategies for patients with LUAD.
Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco
Authors: Zhao Lingmin , Zhang Yanfang , Shao Ying , Xing Linan , Ge Mingran , Huo Xiuwen
Published: December 2022

β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 DoBAM members were identified in yam (Dioscorea opposita Thunb.). A novel β-amylase gene BAM1, (named DoBAM1), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of DoBAM1 is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of DoBAM1 were characterized and found to all be higher than in Bikeqi. DoBAM1 overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and β-amylase. Under cold treatment, we observed the induced upregulation of DoBAM1 and lower starch content and malondialdehyde (MDA) accumulation than in WT plants. In conclusion, these results demonstrate that DoBAM1 overexpression plays an advanced role in cold tolerance, at least in part by raising the levels of soluble sugars that are capable of acting as osmolytes or antioxidants.
Genome-Wide Association Study (GWAS) of White Mold Resistance in Snap Bean
Authors: Arkwazee Haidar A. , Wallace Lyle T. , Hart John P. , Griffiths Phillip D. , Myers James R.
Published: December 2022

White mold can result in snap bean yield losses of 90 to 100% when field conditions favor the pathogen. A genome-wide association study (GWAS) was conducted to detect loci significantly associated with white mold resistance in a panel of snap bean (Phaseolus vulgaris L.) cultivars. Two populations of snap bean were used in this study. The first population was the BeanCAP (Coordinated Agriculture Project) Snap Bean Diversity Panel (SBDP) (n = 136), and the second population was the Snap Bean Association Panel (SnAP) (n = 378). SBDP was evaluated for white mold reaction in the field in 2012 and 2013, and SnAP was screened in a greenhouse only using the seedling straw test in 2016. Two reference genomes representing the Andean and Middle American centers of domestication were utilized to align the genotyping-by-sequencing (GBS) data. A GWAS was performed using FarmCPU with one principal component after comparing five models. Thirty-four single-nucleotide polymorphisms (SNPs) significantly associated with white mold resistance were detected. Eleven significant SNPs were identified by the seedling straw test, and 23 significant SNPs were identified by field data. Fifteen SNPs were identified within a 100 kb window containing pentatricopeptide repeat (PPR)-encoding genes, and eleven were close to leucine-rich repeat (LRR)-encoding genes, suggesting that these two classes are of outsized importance for snap bean resistance to white mold.
Ensuring Nutritional Security in India through Wheat Biofortification: A Review
Authors: Kamble Umesh , Mishra Chandra Nath , Govindan Velu , Sharma Amit Kumar , Pawar Sushma , Kumar Satish , Krishnappa Gopalareddy , Gupta Om Prakash , Singh Gyanendra Pratap , Singh Gyanendra
Published: December 2022

Undernourishment of nutrients, also known as hidden hunger, affects over 2 billion populace globally. Even though stunting among children below five years of age has decreased in India in the last ten years, India is home to roughly thirty percent of the world’s population of stunted pre-schoolers. A significant improvement has been witnessed in the targeted development and deployment of biofortified crops; approximately 20 million farm households from developing counties benefit from cultivating and consuming biofortified crops. There is ample scope for including biofortified varieties in the seed chain, ensuring nutritional security. Wheat is a dietary staple in India, typically consumed as wholemeal flour in the form of flatbreads such as chapatti and roti. Wheat contributes to nearly one fifth of global energy requirements and can also provide better amounts of iron (Fe) and zinc (Zn). As a result, biofortified wheat can serve as a medium for delivery of essential micronutrients such as Fe and Zn to end users. This review discusses wheat biofortification components such as Fe and Zn dynamics, its uptake and movement in plants, the genetics of their buildup, and the inclusion of biofortified wheat varieties in the seed multiplication chain concerning India.
The Expansion of the Spectrum in Stuttering Disorders to a Novel ARMC Gene Family (ARMC3)
Authors: Rehman Adil U , Hamid Malaika , Khan Sher Alam , Eisa Muhammad , Ullah Wasim , Rehman Zia Ur , Khan Muzammil Ahmad , Basit Sulman , Muhammad Noor , Khan Saadullah , Wasif Naveed
Published: December 2022

Stuttering is a common neurodevelopment speech disorder that negatively affects the socio-psychological dimensions of people with disability. It displays many attributes of a complex genetic trait, and a few genetic loci have been identified through linkage studies. Stuttering is highly variable regarding its phenotypes and molecular etiology. However, all stutters have some common features, including blocks in speech, prolongation, and repetition of sounds, syllables, and words. The involuntary actions associated with stuttering often involve increased eye blinking, tremors of the lips or jaws, head jerks, clenched fists, perspiration, and cardiovascular changes. In the present study, we recruited a consanguineous Pakistani family showing an autosomal recessive mode of inheritance. The exome sequencing identified a homozygous splice site variant in ARMC3 (Armadillo Repeat Containing 3) in a consanguineous Pashtun family of Pakistani origin as the underlying genetic cause of non-syndromic stuttering. The homozygous splice site variant (NM_173081.5:c.916 + 1G > A) segregated with the stuttering phenotype in this family. The splice change leading to the skipping of exon-8 is a loss of function (LoF) variant, which is predicted to undergo NMD (Nonsense mediated decay). Here, we report ARMC3 as a novel candidate gene causing the stuttering phenotype. ARMC3 may lead to neurodevelopmental disorders, including stuttering in humans.
Unaffected Li-Fraumeni Syndrome Carrier Parent Demonstrates Allele-Specific mRNA Stabilization of Wild-Type TP53 Compared to Affected Offspring
Authors: Buzby Jeffrey S. , Williams Shirley A. , Nugent Diane J.
Published: December 2022

Li-Fraumeni Syndrome (LFS) is an autosomal dominant disorder where an oncogenic TP53 germline mutation is inherited by offspring of a carrier parent. p53 is a key tumor suppressor regulating cell cycle arrest in response to DNA damage. Unexpectedly, some mutant TP53 carriers remain unaffected, while their children develop cancer early in life. To begin unravelling this paradox, the response of dermal fibroblasts (dFb) isolated from a child with LFS was compared to those from her unaffected father after UV exposure. Phospho-Chk1[S345], a key activator of cell cycle arrest, was increased by UV induction in the LFS patient compared to their unaffected parent dFb. This result, along with previous findings of reduced CDKN1A/p21 UV induction in affected dFb, suggest that cell cycle dysregulation may contribute to cancer onset in the affected LFS subject but not the unaffected parent. Mutant p53 protein and its promoter binding affinity were also higher in dFb from the LFS patient compared to their unaffected parent. These results were as predicted based on decreased mutant TP53 allele-specific mRNA expression previously found in unaffected dFb. Investigation of the potential mechanism regulating this TP53 allele-specific expression found that, while epigenetic promoter methylation was not detectable, TP53 wild-type mRNA was specifically stabilized in the unaffected dFb. Hence, the allele-specific stabilization of wild-type TP53 mRNA may allow an unaffected parent to counteract genotoxic stress by means more characteristic of homozygous wild-type TP53 individuals than their affected offspring, providing protection from the oncogenesis associated with LFS.
Biomarker Discovery for Meta-Classification of Melanoma Metastatic Progression Using Transfer Learning
Authors: Miñoza Jose Marie Antonio , Rico Jonathan Adam , Zamora Pia Regina Fatima , Bacolod Manny , Laubenbacher Reinhard , Dumancas Gerard G. , de Castro Romulo
Published: December 2022

Melanoma is considered to be the most serious and aggressive type of skin cancer, and metastasis appears to be the most important factor in its prognosis. Herein, we developed a transfer learning-based biomarker discovery model that could aid in the diagnosis and prognosis of this disease. After applying it to the ensemble machine learning model, results revealed that the genes found were consistent with those found using other methodologies previously applied to the same TCGA (The Cancer Genome Atlas) data set. Further novel biomarkers were also found. Our ensemble model achieved an AUC of 0.9861, an accuracy of 91.05, and an F1 score of 90.60 using an independent validation data set. This study was able to identify potential genes for diagnostic classification (C7 and GRIK5) and diagnostic and prognostic biomarkers (S100A7, S100A7, KRT14, KRT17, KRT6B, KRTDAP, SERPINB4, TSHR, PVRL4, WFDC5, IL20RB) in melanoma. The results show the utility of a transfer learning approach for biomarker discovery in melanoma.
Ontological Analysis of Coronavirus Associated Human Genes at the COVID-19 Disease Portal
Authors: Wang Shur-Jen , Brodie Kent C. , De Pons Jeffrey L. , Demos Wendy M. , Gibson Adam C. , Hayman G. Thomas , Hill Morgan L. , Kaldunski Mary L. , Lamers Logan , Laulederkind Stanley J. F. , Nalabolu Harika S. , Thota Jyothi , Thorat Ketaki , Tutaj Marek A. , Tutaj Monika , Vedi Mahima , Zacher Stacy , Smith Jennifer R. , Dwinell Melinda R. , Kwitek Anne E.
Published: December 2022

The COVID-19 pandemic stemmed a parallel upsurge in the scientific literature about SARS-CoV-2 infection and its health burden. The Rat Genome Database (RGD) created a COVID-19 Disease Portal to leverage information from the scientific literature. In the COVID-19 Portal, gene-disease associations are established by manual curation of PubMed literature. The portal contains data for nine ontologies related to COVID-19, an embedded enrichment analysis tool, as well as links to a toolkit. Using these information and tools, we performed analyses on the curated COVID-19 disease genes. As expected, Disease Ontology enrichment analysis showed that the COVID-19 gene set is highly enriched with coronavirus infectious disease and related diseases. However, other less related diseases were also highly enriched, such as liver and rheumatic diseases. Using the comparison heatmap tool, we found nearly 60 percent of the COVID-19 genes were associated with nervous system disease and 40 percent were associated with gastrointestinal disease. Our analysis confirms the role of the immune system in COVID-19 pathogenesis as shown by substantial enrichment of immune system related Gene Ontology terms. The information in RGD’s COVID-19 disease portal can generate new hypotheses to potentiate novel therapies and prevention of acute and long-term complications of COVID-19.
The Initial Response to COVID-19 Disruptions for Older People with HIV in Ukraine
Authors: Rozanova Julia , Rich Katherine M. , Altice Frederick L. , Shenoi Sheela V. , Zaviryukha Irina , Kiriazova Tetiana , Mamedova Elmira , Shipunov Oleksandr , Yariy Volodymyr , Deac Alexandra , Zeziulin Oleksandr
Published: December 2022

Ukraine imposed a COVID-19 lockdown in March 2020. From April to June 2020, we surveyed 123 older people with HIV (OPWH) by phone to assess their mental health, engagement in HIV and other healthcare, and substance use using standardised scales. Variables of key interest were symptoms of depression and symptoms of anxiety. Univariate and multivariable Firth logistic regression models were built to assess factors associated with: (1) symptoms of depression, and (2) symptoms of anxiety. Findings indicated high suicidal ideation (10.6%); 45.5% met the screening criteria for moderate to severe depression; and 35.0% met the criteria for generalised anxiety disorder (GAD). Independent correlates of having moderate to severe depression included being female (AOR: 2.83, 95%CI = 1.19–7.05), having concerns about potential barriers to HIV treatment (AOR: 8.90, 95%CI = 1.31–104.94), and active drug use (AOR: 34.53, 95%CI = 3.02–4885.85). Being female (AOR: 5.30, 95%CI = 2.16–14.30) and having concerns about potential barriers to HIV treatment (AOR: 5.33, 95%CI = 1.22–28.45) were independently correlated with GAD, and over half (58.5%) were willing to provide peer support to other OPWH. These results highlight the impact of the COVID-19 restrictions in Ukraine on mental health for OPWH and support the need to screen for psychiatric and substance use disorders, potentially using telehealth strategies.
Long-Term Follow-Up of Intensive Integrative Treatment including Motion Style Acupuncture Treatment (MSAT) in Hospitalized Patients with Lumbar Disc Herniation: An Observational Study
Authors: Park Mu-Jin , Jin So-Ri , Kim Eun-Song , Lee Hyun-Seok , Hwang Kyu-Hyun , Oh Seung-Ju , Lee Jee Young , Kim Doori , Lee Yoon Jae , Ha In-Hyuk
Published: December 2022

This study aimed to investigate the long-term effects of and satisfaction with integrative Korean medicine treatment and motion style acupuncture treatment (MSAT) in patients with lumbar disc herniation (LDH). We retrospectively analyzed medical charts and prospectively surveyed adult patients aged between 19 and 64 years treated for lumbar disc herniation for at least 6 days at three Korean hospitals from 1 January 2015 to 31 December 2020. The primary outcome was the Numeric Rating Scale (NRS) for back pain. Secondary outcome measures included the NRS for radiating leg pain, the Oswestry Disability Index (ODI), and the European Quality of Life-5 Dimension-5 Level (EQ-5D-5L) questionnaire. The NRS scores for low back pain decreased from 5.40 ± 1.58 to 2.92 ± 2.09, NRS for radiating leg pain from 5.57 ± 1.56 to 1.78 ± 2.36, and ODI from 46.39 ± 16.72 to 16.47 ± 15.61 at baseline and survey, respectively. The EQ-5D-5L increased from 0.57 ± 0.19 to 0.82 ± 0.14. In conclusion, Korean medicine and MSAT could be effective treatment methods for patients with LDH. The results of this study can be used as helpful information for clinicians who treat patients with LDH in real clinical settings.
The Effect of Child Trauma on the Relation between Psychological Well-Being and Depressive Symptoms in Chilean University Students
Authors: Barros Paulina , Assar Rodrigo , Botto Alberto , Leighton Caroline , Quevedo Yamil , Jiménez Juan Pablo
Published: December 2022

(1) Background: There is consistent evidence of the impact of early adverse experiences on mental health in adulthood, especially as a risk factor for depression. However, their influence on positive aspects of mental health such as well-being has been less extensively studied. Therefore, this study aims to investigate the effect of traumatic childhood experiences on the relationship between depression and psychological well-being in a sample of university students. (2) Methods: The Childhood Trauma Questionnaire—Short Form (CTQ-SF), the Beck Depression Inventory (BDI-IA), and Ryff’s psychological well-being scale were administered to 700 Chilean university students. Several regression models were used to analyze the interaction between variables, with multivariate SEM being applied to hierarchize the relationships found. (3) Results: Emotional Neglect and Abuse stand out as the types of maltreatment with the greatest impact on mental health, associated first with a decrease in the self-acceptance dimension of psychological well-being and then with depressive symptomatology in adulthood. (4) Conclusions: Results provide evidence that early trauma has an important impact on mental health, increasing the risk of depression, however, its impact is greater on positive aspects of health, such as self-acceptance, a fundamental element in the construction of psychological well-being.
A Multilevel Multiresolution Machine Learning Classification Approach: A Generalization Test on Chinese Heritage Architecture
Authors: Zhang Kai , Teruggi Simone , Ding Yao , Fassi Francesco
Published: December 2022

In recent years, the investigation and 3D documentation of architectural heritage has made an efficient digitalization process possible and allowed for artificial intelligence post-processing on point clouds. This article investigates the multilevel multiresolution methodology using machine learning classification algorithms on three point-cloud projects in China: Nanchan Ssu, Fokuang Ssu, and Kaiyuan Ssu. The performances obtained by extending the prediction to datasets other than those used to train the machine learning algorithm are compared against those obtained with a standard approach. Furthermore, the classification results obtained with an MLMR approach are compared against a standard single-pass classification. This work proves the reliability of the MLMR classification of heritage point clouds and its good generalizability across scenarios with similar geometrical characteristics. The pros and cons of the different approaches are highlighted.
Glass Icons in Transylvania (Romania) and the Craft of Painting Them as Cultural Heritage Resources
Authors: Sorea Daniela , Popescu Ionuț Mihai
Published: December 2022

The craft of painting icons on glass developed in the 17th century in Transylvania (Romania) following the spread of the news about the wooden icon of the weeping Mother of God in the church of Nicula. This news turned Nicula into a pilgrimage centre, and requests for reproductions of the icon led to the locals becoming glass painters. Because of the surplus of icons, some of the Nicula painters set up new icon-painting centres along the road to Brașov (to the south) and the other main trade routes of Transylvania. In order to highlight the potential for sustainable development associated with this craft and to stimulate the painting of icons in the traditional way, we conducted documentary research on the subject of icons on glass. This research revealed the local peculiarities of the icon painters’ workshops and their importance to the identity of the Romanian peasants in Transylvania. We also conducted a participatory observation carried out in Brasov, which revealed that the iconography courses in popular schools of arts and crafts were both a viable way of managing the relationship with the iconographic tradition and a means to capitalize on religious painting on glass as a cultural heritage resource. The research highlighted the way in which, to preserve the traditional dimension of the craft, it is useful to encourage students to use anonymous glass icons as models and to have limited involvement in model restoration.