bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Plant Growth Promotion Under Reduced Phosphate Rates in Two Genotypes of Maize by a Rhizobial Phosphate-Solubilizing Strain
Authors: Beltran-Medina Jose I. , Romero-Perdomo Felipe , Molano-Chavez Lady , Silva Antonio M. M. , Estrada-Bonilla German A.
Published: July 2022

The biotechnological manipulation of phosphate-solubilizing bacteria (PSB) is gaining prominence to improve the poor phosphorus (P) availability in the soil and maintain crop yields. In this study, we investigated howRhizobiumsp. B02 inoculation influences maize crop development and whether its use reduces phosphate fertilizer rates. We conducted growth promotion assays using P fertilizer doses in two maize genotypes under greenhouse conditions. Morphometric, physiological, and productivity parameters were assessed in three phenological stages: tillering (V5), tassel (VT), and maturity (R6). Maize response was significantly influenced by both inoculation and plant genotype, showing that the plant-promoting effect of inoculation is substantially more prominent in the white endosperm than in the yellow endosperm maize genotype. The development of maize in all phenological stages was promoted by inoculation withRhizobiumsp. B02. The most significant influence of inoculation was observed on shoot dry weight, relative chlorophyll content, shoot P concentration, leaf area, photosynthetic rate, 1,000-grain weight, and grain yield. A 17% gain in grain yield, representing 20 g plant−1, was obtained by inoculation with 50% diammonium phosphate (DAP) compared with the control treatment at the same dose. The complete fertilization control was phenocopied by the white endosperm inoculated at 50% DAP in all productivity parameters. Therefore, half of the P fertilization in white endosperm was replaced by inoculation withRhizobiumsp. B02. Herein, we report the potential of aRhizobiumstrain in a non-legume crop to improve P management.
Impact of Surgery on Non-Functional Pancreatic Neuroendocrine Tumors ≤2 cm: Analyses With Propensity Score–Based Inverse Probability of Treatment Weighting
Authors: Ye Jingyuan , Wu Hongyu , Li Jinzheng , Liu Changan
Published: July 2022

Purpose

The impact of surgery on non-functional pancreatic neuroendocrine tumors (NF-PNETs) ≤2 cm is controversial. This study sought to demonstrate the impact of surgery on the prognosis of NF-PNETs ≤2 cm with different biological behaviors.

Methods

Patients with NF-PNETs ≤2 cm from 2004 to 2015 in the Surveillance, Epidemiology, and End Results database were included in this study. An inverse probability of treatment weighting (IPTW) method was used to reduce the selection bias. Kaplan–Meier survival analysis and Cox proportional hazards regression were used to evaluate the effect of surgery on the prognosis.

Results

In the IPTW-adjusted Cox proportional hazards regression analysis, surgery improved the cancer-specific survival (CSS) in the overall cohort (hazard ratio [HR], 0.187; 95% confidence interval [CI], 0.102–0.343; p < 0.001), patients with poorly differentiated or undifferentiated tumor grades (HR, 0.238; 95% CI, 0.105–0.64; p < 0.001), patients with distant metastasis (HR, 0.102; 95% CI, 0.021–0.496; p = 0.005), and patients with local invasion (HR, 0.059; 95% CI, 0.005–0.683; p = 0.002). Surgery did not improve the CSS in patients with lymph node metastasis only (HR, 0.26; 95% CI, 0.0462–1.461; p = 0.126) or patients with well or moderate differentiation while without distant and lymph node metastasis (HR, 0.387; 95% CI, 0.146–1.028; p = 0.057).

Conclusions

Among patients with NF-PNETs ≤2 cm, different biological behaviors correlate with different prognostic impacts of surgery. As long as distant metastasis does not occur and the grade is well–moderately differentiated, these patients will not benefit from surgery no matter whether lymph node metastasis occurs or not. However, when local invasion appears in this group of patients, surgery should be performed. Moreover, patients with a tumor grade of poorly differentiated or undifferentiated or those with distant metastases may benefit from surgery.
Intraoperative Identification of the Intersegmental Plane: From the Beginning to the Future
Authors: Zhang Xianfei , Li Chengqiang , Jin Runsen , Li Hecheng
Published: July 2022

Segmentectomy has played a crucial role in the treatment of early-stage lung cancer after the publication of JCOG0802, which indicated that patients with small-sized peripheral non-small-cell lung cancer could receive better survival from segmentectomy than lobectomy despite a higher local recurrence. The intraoperative identification of the intersegmental plane ensures complete resection of the lesion with sufficient margin so that it is deemed as the critical part of segmentectomy. Diverse methods have been developed to acquire distinguishable and lasting borderline between segments, but none of them is proved perfect. In this review, we searched and classified these techniques that emerged from the beginning when segmentectomy was used for bronchiectasis until now. Comparisons between different ways in mechanisms, facility, and safety were made to depict a comprehensive landscape for surgeons to select fit one. Furthermore, we presented our vision for the future of intersegmental plane identification.
Distribution of Cannabinoid Receptors in Keratinocytes of Healthy Dogs and Dogs With Atopic Dermatitis
Authors: Chiocchetti Roberto , De Silva Margherita , Aspidi Francesca , Cunha Rodrigo Zamith , Gobbo Francesca , Tagliavia Claudio , Sarli Giuseppe , Morini Maria
Published: July 2022

It is commonly accepted that some form of skin barrier dysfunction is present in canine atopic dermatitis (AD), one of the most common cutaneous pruritic inflammatory diseases of dogs. The impaired skin barrier function facilitates the penetration of allergens and subsequently stronger sensitization responses. The role of the endocannabinoid system (ECS) in the physiology and pathology of the skin is becoming increasingly established. It has been demonstrated that cannabinoid receptors are expressed in healthy and diseased skin and, based on current knowledge, it could be stated that cannabinoids are important mediators in the skin. The present study has been designed to immunohistochemically investigate the expression of the cannabinoid receptors type 1 (CB1R) and 2 (CB2R) and the cannabinoid-related receptors G protein-coupled receptor 55 (GPR55), transient receptor potential vanilloid 1 (TRPV1) and ankyrin 1 (TRPA1), peroxisome proliferator-activated receptors alpha (PPARα), and serotoninergic receptor 1a (5-HT1aR) in keratinocytes of healthy dogs and of dogs with AD. Samples of skin tissues were collected from 7 healthy controls (CTRL-dogs) and from 8 dogs with AD (AD-dogs). The tissue samples were processed using an immunofluorescence assay with commercially available antibodies, and the immunolabelling of the receptors studied was quantitatively evaluated. The keratinocytes of the CTRL- and the AD-dogs showed immunoreactivity for all the receptors investigated with a significant upregulation of CB2R, TRPA1, and 5-HT1aR in the epidermis of the AD-dogs. The presence of cannabinoid and cannabinoid-related receptors in healthy keratinocytes suggested the possible role of the ECS in canine epidermal homeostasis while their overexpression in the inflamed tissues of the AD-dogs suggested the involvement of the ECS in the pathogenesis of this disease, having a possible role in the related skin inflammation and itching. Based on the present findings, the ECS could be considered a potential therapeutic target for dogs with AD.
Case Report: Disorder of Sexual Development in a Chinese Crested Dog With XX/XY Leukocyte Chimerism and Mixed Cell Testicular Tumors
Authors: Schwartz Rebecca , Sugai Nicole J. , Eden Kristin , Castaneda Caitlin , Jevit Matthew , Raudsepp Terje , Cecere Julie T.
Published: July 2022

A 10-year-old intact female Chinese Crested dog was presented for evaluation and further diagnostics due to persistent symptoms of vulvar swelling, vaginal discharge, and an 8-year history of acyclicity. At presentation, generalized hyperpigmentation and truncal alopecia were identified, with no aberrations of the female phenotype. Vaginal cytology confirmed the influence of estrogen at multiple veterinary visits, and hormonal screening of progesterone and anti-Mullerian hormone indicated gonadal presence. Based on findings from abdominal laparotomy and gonadectomy, the tissue was submitted for histopathology. Histopathologic evaluation identified the gonads to be abnormal testes containing multiple Sertoli and interstitial (Leydig) cell tumors. The histopathologic diagnosis of testes and concurrent normal external female phenotype in the patient lead to a diagnosis of a disorder of sexual development (DSD). Karyotype evaluation by conventional and molecular analysis revealed a two cell line chimeric pattern of 78,XX (80%) and 78,XY (20%) among blood leukocytes, as well as a positive PCR test for the Y-linked SRY gene. Cytogenetic analysis of skin fibroblasts revealed the presence of 78,XX cells exclusively, and PCR tests for the Y-linked SRY gene were negative in the hair and skin samples. These results are consistent with an XX/XY blood chimerism. This is one of the few case reports of a canine with the diagnosis of leukocyte chimerism with normal female phenotypic external genitalia. This case illustrates a distinct presentation for hormonally active Sertoli cell tumorigenesis and demonstrates surgery as a curative treatment option for clinically affected patients.
Prevalence and Consequences of Verbal Aggression among Bank Workers: A Survey into an Italian Banking Institution
Authors: Acquadro Maran Daniela , Varetto Antonella , Civilotti Cristina , Magnavita Nicola
Published: July 2022

Robberies and other violent acts by strangers and intruders are common in banks, which can lead to post-traumatic stress disorder in employees. However, the literature indicates that more subtle and less measurable forms of violence, such as psychological violence, can also cause discomfort among employees. The aim of the present work is to investigate the prevalence of verbal aggression and its consequences among 311 Italian bank employees. A self-administered questionnaire was completed by 197 employees. The results showed a high prevalence of verbal aggression in the sample. It is important to investigate and find the causes and effects of verbal aggression in order to understand the phenomenon in this workplace. Cases of verbal aggression need to be prevented and responded to appropriately. In addition, it should be noted that the effects of verbal aggression do not only affect the victim, but also the workplace, as productivity decreases, and the feeling of a safe environment is missing.
A Light Recipe including Far-Red Wavelength during Healing of Grafted Watermelon Seedlings Enhances the Floral Development and Yield Earliness
Authors: Bantis Filippos , Gkotzamani Anna , Dangitsis Christodoulos , Koukounaras Athanasios
Published: July 2022

Watermelon is widely propagated through grafting, after which seedlings are subjected to healing under controlled conditions including artificial lighting. Light wavelengths, such as blue, red, and far-red, impose considerable effects on seedlings, which possibly carry on to the mature plants. The aim of the present study is to examine whether different light wavelengths during healing of grafted watermelon seedlings impose variable effects during field cultivation. After grafting, seedlings were healed in an environmentally controlled healing chamber under fluorescent (FL) lamps and light-emitting diodes, providing 100% red (R), 100% blue (B), 88/12% R/B (12B), and 12B including 5% far-red (12B + FR). After acclimatization, seedlings were transplanted in the field. Vegetative growth until floral initiation was enhanced by 12B and 12B + FR, as shown by stem diameter and leaf number measurements. Flowering was mainly accelerated by 12B + FR and considerably decelerated by FL and B. The same pattern was followed by fruit yield, which was similar for all treatments at the end of the experiment. Nevertheless, fruit quality was not affected by any of the light treatments. It is concluded that a light recipe, including red, blue and far-red, wavelengths during healing of grafted seedlings enhances the overall growth, and flowering and yield earliness of watermelon crops.
The Metabolomics Approaches Based on LC-MS/MS for Analysis of Non-Halal Meats in Food Products: A Review
Authors: Windarsih Anjar , Rohman Abdul , Riswanto Florentinus Dika Octa , Dachriyanus  , Yuliana Nancy Dewi , Bakar Nor Kartini Abu
Published: July 2022

Halal meats are meats that are allowed to be consumed by Muslim societies according to Islamic law (Syariah). Due to the development of food technology, non-halal meats such as pork or canine meat are added to food products to reduce the production costs. Non-halal meats also include meats from animals which are not slaughtered according to Syariah law; therefore, the availability of a standardized analytical method capable of detecting the presence of non-halal meats with high sensitivity is very urgent. The metabolomics technique, either targeted or untargeted approaches based on liquid chromatography–tandem mass spectrometry (LC-MS/MS) measurements is an emerging analytical method applied to the identification of non-halal meats in food products. The LC-MS/MS measurements provide an enormous metabolomics data, therefore, sophisticated data analysis tools such as chemometrics is required. Among the chemometrics techniques, exploratory data analysis for supervised and unsupervised pattern recognition, including principal component analysis (PCA), hierarchical cluster analysis (HCA), and linear-discriminant analysis (LDA), are the most-used. This review focused on the recent application of LC-MS/MS in combination with chemometrics for the detection and identification (qualitative analysis) of non-halal meats in food products. The selection criteria used for the papers in this review were studies on the application of metabolomics using LC-MS/MS and chemometrics for the halal authentication of meat products between 2005 and 2022. The results showed that potential biomarkers of non-halal meats could be found using chemometrics analysis. Therefore, it can be concluded that a combination of LC-MS/MS and chemometrics is promising for development as a standard analytical method for the analysis of non-halal meats in food products.
Growth and Fruit Yields of Greenhouse Tomato under the Integrated Water and Fertilizer by Moistube Irrigation
Authors: Zhang Mingzhi , Xiao Na , Li Yangjian , Li Yuan , Zhang Dong , Xu Zhijing , Zhang Zhenxing
Published: July 2022

The mechanism of greenhouse tomato growth and yield under the integrated water and fertilizer of moistube irrigation (MI) is not clear. Thus, to fill the research gap, a completely randomized trial design was used to study the effects of different irrigation amounts (I; to realize different I, the tube working pressure was 1 (I1), 2 (I2), 3 (I3) m) and fertilizer amounts (F, N-P-K: 20%-20%-20%; the F at a single time was 100 (F1), 200 (F2) and 300 (F3) kg/ha) on growth and yield of tomato. The results showed that with an increase in I, the photosynthetic rate (Pn) of leaves and total dry matter mass (TDM) first increased and then decreased, while the nutrition and the flavor indexes of fruit decreased. With an increase in F, the Pn of leaves, the TDM of tomato and the fruit quality increased at first and then decreased. The effects of I on the yield of tomato was higher than that of F. With an increase in I, the partial fertilizer productivity (PFP) increased at first and then decreased, and the water use efficiency (WUE) decreased by 13.96%. With an increase in F, the WUE increased at first and then decreased, and the PFP decreased by 148.97%. The conclusion based on a spatial analysis was consistent with the comprehensive evaluation of yield and water use efficiency, which showed that I2F2 was the best.
Reproductive Hormones Mediate Intestinal Microbiota Shifts during Estrus Synchronization in Grazing Simmental Cows
Authors: Wu Donglin , Wang Chunjie , Simujide Huasai , Liu Bo , Chen Zhimeng , Zhao Pengfei , Huangfu Mingke , Liu Jiale , Gao Xin , Wu Yi , Li Xiaorui , Chen Hao , Chen Aorigele
Published: July 2022

To study shifts in the intestinal microbiota during estrus synchronization in ruminants, we characterized the intestinal microbiota in grazing Simmental cows and the possible mechanism that mediates this shift. Fourteen postpartum Simmental beef cows were synchronized beginning on day 0 (D0) with a controlled internal release device (CIDR), and cloprostenol was injected on D9 when the CIDR was withdrawn. Synchronization ended with timed artificial insemination on D12. Serum and rectal samples harvested on D0, D9, and D12 were analyzed to assess the reproductive hormones and microbiota. Reproductive hormones in the serum of the host were measured using enzyme-linked immunosorbent assay. The microbiota was characterized using 16S rRNA sequencing of the V3–V4 hypervariable region, alpha diversity and beta diversity analyses (principal coordinate analysis, PCoA), cladogram of the linear discriminant analysis effect size (LEfSe) analysis, and microbiota function analysis. Levels of the reproductive hormones, except gonadotropin-releasing hormone (p > 0.05), shifted among D0, D9, and D12 (p < 0.05). Decreased community diversity (Chao1 and ACE) was observed on D12 compared with D0 (p < 0.05). The beta diversity (PCoA) of the microbiota shifted markedly among D0, D9, and D12 (p < 0.05). The LEfSe analysis revealed shifts in the intestinal microbiota communities among D0, D9, and D12 (p < 0.05 and LDA cutoff >3.0). The KEGG pathway analysis showed that carbohydrate metabolism, genetic information and processing, the excretory system, cellular processes and signaling, immune system diseases, and the metabolism were altered (p < 0.05). Reproductive hormones (especially estradiol) were correlated with the alpha diversity indices, beta diversity indices, and an abundance of biomarkers of the shifting intestinal microbiota (p < 0.05). In conclusion, the structure, composition, and function of the intestinal microbiota were shifted during estrus synchronization in a grazing Simmental cow model, and these shifts were mediated by reproductive hormones.
The Development of a Multiplex Real-Time Quantitative PCR Assay for the Differential Detection of the Wild-Type Strain and the MGF505-2R, EP402R and I177L Gene-Deleted Strain of the African Swine Fever Virus
Authors: Zhao Kang , Shi Kaichuang , Zhou Qingan , Xiong Chenyong , Mo Shenglan , Zhou Hongjin , Long Feng , Wei Haina , Hu Liping , Mo Meilan
Published: July 2022

African swine fever virus (ASFV) causes African swine fever (ASF), a devastating hemorrhagic disease of domestic pigs and wild boars. Currently, the MGF505R, EP402R (CD2v) and I177L gene-deleted ASFV strains were confirmed to be the ideal vaccine candidate strains. To develop an assay for differentiating the wild-type and gene-deleted ASFV strains, four pairs of specific primers and TaqMan probes targeting the ASFV B646L (p72), I177L, MGF505-2R and EP402R (CD2v) genes were designed. A multiplex real-time qPCR assay for the differential detection of the wild-type and gene-deleted ASFV strains was developed after optimizing the reaction conditions, including the annealing temperature, primer concentration and probe concentration. The results showed that the multiplex real-time qPCR assay can specifically test the ASFV B646L (p72), I177L, MGF505-2R and EP402R (CD2v) genes with a limit of detection (LOD) of 32.1 copies/μL for the B646L (p72) gene, and 3.21 copies/μL for the I177L, MGF505-2R and EP402R (CD2v) genes. However, the assay cannot test for the classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), porcine circovirus type 2 (PCV2), PCV3 and pseudorabies virus (PRV). The assay demonstrated good repeatability and reproducibility with coefficients of variation (CV) less than 1.56% for both the intra- and inter-assay. The assay was used to test 4239 clinical samples, and the results showed that 12.60% (534/4239) samples were positive for ASFV, of which 10 samples lacked the EP402R gene, 6 samples lacked the MGF505-2R gene and 14 samples lacked the EP402R and MGF505-2R genes. The results indicated that the multiplex real-time qPCR developed in this study can provide a rapid, sensitive and specific diagnostic tool for the differential detection of the ASFV B646L, I177L, MGF505-2R and EP402R genes.
Impacts of Reducing Protein Content in Milk Replacer on Growth Performance and Health of Young Calves
Authors: Schubert Dana Carina , Chuppava Bussarakam , Hoffmans Sandra , Pries Martin , Visscher Christian , Kamphues Josef , Abd El-Wahab Amr
Published: July 2022

In the present study, a drinking amount of 10 L of milk replacer (MR) was allowed to dairy calves in order to approach the natural drinking behavior. The question is: how much protein is required by calves in order to achieve an intended growth rate? For this reason, sixty-eight pre-weaned Holstein calves were divided into two groups and fed with 10 L/d of MR containing either 22% protein (MR22) or 19% protein (MR19) at an almost comparable energy intake. Effects on performance, metabolic status, and health were compared. Feed intake, growth performance, and health status were monitored during the pre-transition, transition, and postweaning phase (until 157 d of age). Total feed intake, and intake of MR, body weight (BW), and average daily gain (ADG) were not significantly different between MR22 and MR19 during the entire experimental period (p > 0.05). At d 42, calves in MR19 group showed greater serum levels of growth hormone (16.2 vs. 22.2 ng/mL; p = 0.02), insulin-like growth factor 1 (262 vs. 291 ng/mL; p = 0.03), and urea (2.86 vs. 3.04 mmol/L; p < 0.01). The results of the present study suggested that when high amounts of MR are provided, the protein content in MR can be reduced to 19% without any adverse effects on growth performance as well as on health status of dairy calves.
Introducing Mediterranean Lupins in Lamb Diets: Effects on Carcass Composition, Meat Quality, and Intramuscular Fatty Acid Profile
Authors: Almeida Mariana , Garcia-Santos Sofia , Carloto Daniela , Arantes André , Lorenzo Jose M. , Silva José António , Santos Virgínia , Azevedo Jorge , Guedes Cristina , Ferreira Luís , Silva Severiano
Published: July 2022

The objective of this preliminary study was to evaluate the effects of partial replacement of soybean meal by lupins on lambs’ diets, on the carcass traits, meat characteristics, and meat fatty acid profile. Two trials were conducted: In trial 1, the soybean meal (control; C) was partially replaced by Lupinus albus or Lupinus luteus (50 g/kg; LA5 and LL5, respectively); in trial 2, lambs were fed four diets with graded levels of Lupinus luteus (0, 100, 150 and 200 g/kg; C, LL10, LL15, LL20, respectively). At the end of the feeding trials, animals were slaughtered to evaluate carcass characteristics and meat composition, including fatty acids. Carcass composition in tissues was not affected (p > 0.05) by diet in both trials. Additionally, no significant (p < 0.05) differences were observed in meat quality attributes between diets in trials 1 and 2. Overall, the Longissimus muscle’s fatty acid content was not affected by diet (p > 0.05) in both trials. Carcass and meat quality was overall comparable between lambs fed with soybean meal and lupins, indicating the latter as a potential alternative protein source. However, the lack of significant differences could also be attributed to the small sample size.
Novel 1,2,3-Triazole-sulphadiazine-ZnO Hybrids as Potent Antimicrobial Agents against Carbapenem Resistant Bacteria
Authors: Aljohani Faizah S. , Rezki Nadjet , Aouad Mohamed R. , Hagar Mohamed , Bakr Basant A. , Shaaban Marwa M. , Elwakil Bassma H.
Published: July 2022

Bacterial pneumonia is considered one of the most virulent diseases with high morbidity and mortality rates, especially in hospitalized patients. Moreover, bacterial resistance increased over the last decades which limited the therapy options to carbapenem antibiotics. Hence, the metallo-β-lactamase-producing bacteria were deliberated as the most deadly and ferocious infectious agents. Sulphadiazine-ZnO hybrids biological activity was explored in vitro and in vivo against metallo-β-lactamases (MBLs) producing Klebsiella pneumoniae. Docking studies against NDM-1 and IMP-1 MBLs revealed the superior activity of the 3a compound in inhibiting both MBLs enzymes in a valid reliable docking approach. The MBLs inhibition enzyme assay revealed the remarkable sulphadiazine-ZnO hybrids inhibitory effect against NDM-1 and IMP-1 MBLs. The tested compounds inhibited the enzymes both competitively and noncompetitively. Compound 3b-ZnO showed the highest antibacterial activity against the tested metallo-β-lactamase producers with an inhibition zone (IZ) diameter reaching 43 mm and a minimum inhibitory concentration (MIC) reaching 2 µg/mL. Sulphadiazine-ZnO hybrids were tested for their in vitro cytotoxicity in a normal lung cell line (BEAS-2Bs cell line). Higher cell viability was observed with 3b-ZnO. Biodistribution of the sulphadiazine-ZnO hybrids in the lungs of uninfected rats revealed that both [124I]3a-ZnO and [124I]3b-ZnO hybrids remained detectable within the rats’ lungs after 24 h of endotracheal aerosolization. Moreover, the residence duration in the lungs of [124I]3b-ZnO (t1/2 4.91 h) was 85.3%. The histopathological investigations confirmed that compound 3b-ZnO has significant activity in controlling bacterial pneumonia infection in rats.
Photodynamic Therapy for Peri-Implant Diseases
Authors: Rahman Betul , Acharya Anirudh Balakrishna , Siddiqui Ruqaiyyah , Verron Elise , Badran Zahi
Published: July 2022

Peri-implant diseases are frequently presented in patients with dental implants. This category of inflammatory infections includes peri-implant mucositis and peri-implantitis that are primarily caused by the oral bacteria that colonize the implant and the supporting soft and hard tissues. Other factors also contribute to the pathogenesis of peri-implant diseases. Based on established microbial etiology, mechanical debridement has been the standard management approach for peri-implant diseases. To enhance the improvement of therapeutic outcomes, adjunctive treatment in the form of antibiotics, probiotics, lasers, etc. have been reported in the literature. Recently, the use of photodynamic therapy (PDT)/antimicrobial photodynamic therapy (aPDT) centered on the premise that a photoactive substance offers benefits in the resolution of peri-implant diseases has gained attention. Herein, the reported role of PDT in peri-implant diseases, as well as existing observations and opinions regarding PDT, are discussed.
The RNA Polymerase Inhibitor Corallopyronin A Has a Lower Frequency of Resistance Than Rifampicin in Staphylococcus aureus
Authors: Balansky Jan , Pfarr Kenneth , Szekat Christiane , Kehraus Stefan , Aden Tilman , Grosse Miriam , Jansen Rolf , Hesterkamp Thomas , Schiefer Andrea , König Gabriele M. , Stadler Marc , Hoerauf Achim , Bierbaum Gabriele
Published: July 2022

Corallopyronin A (CorA) is active against Gram-positive bacteria and targets the switch region of RNA polymerase. Because of the high frequency of mutation (FoM) leading to rifampicin resistance, we determined the CorA FoM in S. aureus using fluctuation analysis at 4 × minimum inhibitory concentration (MIC). Resistant mutants were characterized. S. aureus strains HG001, Mu50, N315, and USA300 had an MIC of 0.25 mg/L. The median FoM for CorA resistance was 1.5 × 10−8, 4.5-fold lower than the median FoM of 6.7 × 10−8 for rifampicin, and was reflected in a 4-fold lower mutation rate for CorA than rifampicin (6 × 10−9 for CorA vs. 2.5 × 10−8 for rifampicin). In CorA-resistant/rifampicin-sensitive strains, the majority of amino acid exchanges were S1127L in RpoB or K334N in RpoC. S. aureus Mu50, a rifampicin-resistant clinical isolate, yielded two further exchanges targeting amino acids L1131 and E1048 of the RpoB subunit. The plating of >1011 cells on agar containing a combination of 4 × MIC of rifampicin and 4 × MIC of CorA did not yield any growth. In conclusion, with proper usage, e.g., in combination therapy and good antibiotic stewardship, CorA is a potential antibiotic for treating S. aureus infections.
7-O-Methylluteolin Suppresses the 2,4-Dinitrochlorobenzene-Induced Nrf2/HO-1 Pathway and Atopic Dermatitis-like Lesions
Authors: Kim Tae-Young , Park No-June , Jo Beom-Geun , Paik Jin-Hyub , Choi Sangho , Kim Su-Nam , Yang Min Hye
Published: July 2022

7-O-methylluteolin (7-ML) is a flavonoid isolated from the aerial parts of Wikstroemia ganpi (W. ganpi). We describe the anti–atopic dermatitis (AD) effects of 7-ML in tert-butyl hydroperoxide (tBHP)-induced HepG2 cells and 2,4-dinitrochlorobenzene (DNCB)-induced SKH-1 hairless mice. Results demonstrated that 7-ML dose-dependently inhibited the activation of Nrf2 (nuclear factor-erythroid 2-related factor 2) in tBHP-induced HepG2 cells. 7-ML applied topically to our DNCB-induced mouse model upregulated the antioxidant protein expression (phosphorylated Nrf2 (pNrf2), Nrf2, and heme oxygenase-1 (HO-1)) in skin tissues, improved epidermal thickness, and reduced mast cell infiltration into the skin. In addition, 7-ML reduced the serum levels of immunoglobulin E (IgE) and interleukin-4 (IL-4) and improved skin barrier functions. These results suggest that 7-ML should be considered a novel antioxidant and anti-AD agent.
Cruise Industry Trends and Cruise Ships’ Navigational Practices in the Central and South Part of the Adriatic East Coast Affecting Navigational Safety and Sustainable Development
Authors: Dorigatti Josip , Perić Tina , Mrčelić Gorana Jelić
Published: July 2022

The analysis of cruising trends in the Mediterranean regions shows that the Adriatic is the fastest growing cruise region in terms of the number of passenger movements and cruise ships’ port calls among all regions, particularly the central and south part of the east Adriatic coast. The aim of the paper is to analyze leading cruise destination trends in the central and south part of the Adriatic east coast, as well as to identify newly established cruise ships routes, define high-risk navigational and environmental areas and determine cruise traffic density in the vicinity of marine protected areas. The analyses of leading cruise destinations trends are based on four-year (from 2015 to 2019) cruise passenger movement and cruise calls data, whereas analyses of cruise traffic movement are based on one-year cruise ships traffic monitoring (from August 2014 to July 2015). The results of the cruise ship traffic analysis show that cruise ships frequently pass through areas of high navigational and environmental risks that are geographically restricted, navigationally challenging and environmentally sensitive. These routes have become standard navigational practice in newly discovered cruising regions. The obtained results offer a general overview of high-risk cruise ships’ navigational practices in coastal navigation that can be associated with any coastal region in the world.
Effect of Scents on Gazing Behavior and Choice
Authors: Szakál Dorina , Fehér Orsolya , Radványi Dalma , Gere Attila
Published: July 2022

Environmental stimuli can have a significant impact on our decisions. Elements of the store atmosphere, such as music, lights and smells, all have effects on choices, but these have been only vaguely investigated. In the present study, we aim to uncover the effect of strawberry scent on the gazing behavior and choices of the 62 recruited participants. A static eye-tracker was used to study the effect of scent, released by a diffuser. In total, 31 participants completed the study under odorless conditions, while another 31 participants had strawberry fragrance sprayed into the air. The objectives of the study were (1) to determine whether the most gazed-upon product in each of the four categories (chocolate, tea, muesli bar, yoghurt) was chosen, (2) whether the presence of the strawberry scent influenced consumer decision making, i.e., whether the strawberry scent influenced more people to choose strawberry-flavored products, and (3) to introduce the application of a fast and easy-to-use technique for the qualitative analysis of strawberry aroma present in the air during eye-tracking measurements. The results show that (1) participants chose the product they had studied the longest, for all four categories, and (2) the presence or absence of the scent had no significant effect on choice, with the same frequencies of choosing each product in the two conditions regardless of the flavor of the products.
Age-Related Study and Collision Response of Material Properties of Long Bones in Chinese Pedestrian Lower Limbs
Authors: Zeng Yong , Meng Qingnan , Chen Yijiu , Zou Donghua , Tao Luyang
Published: July 2022

In forensic examination cases, lower limb injuries are common, and pedestrians of different ages suffer different injuries when they are hit by vehicles, especially the injuries to the long bones of the lower limbs. Aging remains a challenging issue for the material properties and injury biomechanical properties of pedestrian lower limb long bones. We analyzed the regression relationship between the age of 50 Chinese pedestrians and the material properties of the lower limb long bones (femur, tibia). We compared them with previous studies to propose a regression model suitable for Chinese human long bone material properties. Through the established Human Active Lower Limb (HALL) model that conforms to the Chinese human anatomy, seven pedestrians’ (20/30/40/50/60/70/80 years old (YO)) lower limbs were parameterized to assign long bone material properties. In the finite element analysis, the Hall model was side-impacted by a family car (FCR) at speeds of 30/40/50/60/70 km/h, respectively. The results showed that an increase in age was negatively correlated with a decrease in the material properties of each long bone. Moreover, with an increase in age, the tolerance limit of long bones gradually decreases, but there will be a limit, and there is no obvious positive correlation with age. During a standing side impact, the stress change in the femur was significantly smaller than that of the tibia, and the stress of the femur and tibia decreased with age. Age is a more significant influencing factor for lower limb injuries. Older pedestrians have a higher risk of lower limb injuries. Forensic experts should pay attention to the critical factor of age when encountering lower limb traffic accident injuries in forensic identification work.
A Possible Information Entropic Law of Genetic Mutations
Authors: Vopson Melvin M.
Published: July 2022

The current scientific consensus is that genetic mutations are random processes. According to the Darwinian theory of evolution, only natural selection determines which mutations are beneficial in the course of evolution, and there is no deterministic correlation between any parameter and the probability that these mutations will occur. Here, we investigate RNA genetic sequences of the SARS-CoV-2 virus using Shannon’s information theory, and we report a previously unobserved relationship between the information entropy of genomes and their mutation dynamics. Based on the analysis presented here, we are able to formulate a governing law of genetic mutations, stating that genomes undergo genetic mutations over time driven by a tendency to reduce their overall information entropy, challenging the existing Darwinian paradigm.
An Experimental Demonstration of MIMO C-OOK Scheme Based on Deep Learning for Optical Camera Communication System
Authors: Nguyen Van Linh , Tran Duc Hoang , Nguyen Huy , Jang Yeong Min
Published: July 2022

Currently, wireless communication systems that use radio frequency are commonly deployed, for example, mobile communication systems, satellite systems, and the Internet of Things (IoT) systems. Based on their easy installation, wireless communication systems have benefits over other wired communication systems. However, using high frequencies to transfer data via wireless communication can hold significant risks for human health. Several researchers have studied this topic using visible light instead of Radio Frequency (RF) waveforms in communication systems. Many potential approaches are relevant in this regard, i.e., visible light communication, light fidelity, free-space optical, and optical camera communication. Artificial intelligence is also influencing the future of industry and people and is used to solve complex problems, create intelligent solutions, and replace human intelligence as the driving force behind emerging technologies such as big data, smart factories, and the IoT. In this paper, we proposed the architecture of the MIMO C-OOK (Multiple-Input Multiple-Output Camera On–Off Keying) scheme, which uses a convolutional neural network for light-emitting diode detection and a deep learning neural network for threshold predictions considering long-distance communication and mobility support. Our suggested method aimed to improve the performance of the traditional camera on–off keying scheme by increasing data rate, communication distance, and low bit error rate. Our suggested technique may achieve a communication distance of up to 22 m with a low error rate when considering the mobility impact (2 m/s, i.e., walking velocity) by controlling the exposure time, focal length, and employing Forward Error Correction code.
The Effect of Side Wall Cutout Sizes on Corrugated Box Compression Strength in the Function of Length-to-Width Ratios—An Experimental Study
Authors: Pidl Renáta , Fehér Lajos , Böröcz Péter
Published: July 2022

Packaging made from corrugated cardboard is a widely used solution in modern supply chains for the handling, storage and distribution of goods. These packages are required to maintain adequate protection conditions; however, in many cases, the cardboard box dimensions, handles and/or ventilation holes, quality and their configuration could compromise its protection strength. This study observes and evaluates the performance of corrugated cardboard boxes made with B-flute boards by considering different cutout sizes from the side walls (0%, 20%, 40%, 60% and 80%) in various box length–width ratios of 200 mm, 300 mm, 400 mm, 500 mm and 600 mm in length and a constant 300 mm width and height. Box compression tests were performed in a laboratory, and results were compared with mathematical statistics. In each cutout case, the maximum compression force was observed with the box with dimensions of 400 × 300 × 300 mm. The measurement results showed that the 1.33 length-to-width ratio has the best maximum compression force result. The statistical tests showed that there is no significant difference between the 0% and 20% cutout groups.
Numerical Analysis of a Full-Scale Thermophilic Biological System and Investigation of Nitrate and Ammonia Fates
Authors: Collivignarelli Maria Cristina , Pedrazzani Roberta , Bellazzi Stefano , Carnevale Miino Marco , Caccamo Francesca Maria , Baldi Marco , Abbà Alessandro , Bertanza Giorgio
Published: July 2022

Thermophilic biological processes proved to be effective in aqueous waste (AW) and high-strength wastewater treatment. In this work, the monitoring of a full-scale aerobic thermophilic biological plant treating various high-strength AW in continuous mode is reported. This paper aims to: (i) provide models to help the AW utility manager in predicting the load of fed pollutants and performances, and (ii) fully investigate nitrogen transformations in biological reactor. Based on the results, the thermophilic sludge in the studied plant was able to degrade Chemical Oxygen Demand (COD) and remove nitrate nitrogen with very high efficiency (79.3% and 97.1, respectively). The monitoring was conducted following a statistical approach and searched for the possible correlations between the input parameters and the efficiency of removal of the plant. Moreover, a multivariate linear regression was carried out highlighting that the yield value of the removal of COD and nitrogen forms, apart from ammonia, was well explained (R2 = 0.9) by the linear regression against the other monitored parameters. As far as nitrification is concerned, there was, on the one hand, an increase in ammonium ions due to the hydrolysis of the organic substance that occurs in the reactor, and on the other hand, a stripping of the same ammoniacal nitrogen in the form of NH3. While nitrates were effectively removed, according to fluorescent in situ hybridization tests, sludge proved to be formed by minute flocs, where bacteria responsible for the oxidation of ammonium and nitrite seem to be unable to grow.
A Review of Collaborative Virtual Reality Systems for the Architecture, Engineering, and Construction Industry
Authors: Ververidis Dimitrios , Nikolopoulos Spiros , Kompatsiaris Ioannis
Published: July 2022

In this paper, we focus on interdisciplinary collaboration using intuitive virtual reality interfaces and building information models in the architecture, engineering, and construction industries. These systems have been a topic of research and development for the past ten years; however, there is still no widely open standard format, related software platform, or guidelines that are sufficiently mature; the complexity of such systems is very high. We review existing state-of-the-art interdisciplinary collaborative virtual reality systems, proposing solutions and standards. Thirteen state-of-the-art systems are reviewed and compared to illustrate emerging trends and insufficiencies. It is found that these systems differ significantly with respect to drawing capabilities, photorealism, construction simulation, and interdisciplinary communication. We discover trends in user interfaces that could be evolved to better standards, and provide future guidelines to developers. Combining the best aspects of existing systems, we provide a blueprint for an ideal system that combines the most advanced features for collaborative design.
Effect of Heel Lift Insoles on Lower Extremity Muscle Activation and Joint Work during Barbell Squats
Authors: Lu Zhenghui , Li Xin , Xuan Rongrong , Song Yang , Bíró István , Liang Minjun , Gu Yaodong
Published: July 2022

The effect of heel elevation on the barbell squat remains controversial, and further exploration of muscle activity might help find additional evidence. Therefore, 20 healthy adult participants (10 males and 10 females) were recruited for this study to analyze the effects of heel height on lower extremity kinematics, kinetics, and muscle activity using the OpenSim individualized musculoskeletal model. One-way repeated measures ANOVA was used for statistical analysis. The results showed that when the heel was raised, the participant’s ankle dorsiflexion angle significantly decreased, and the percentage of ankle work was increased (p < 0.05). In addition, there was a significant increase in activation of the vastus lateralis, biceps femoris, and gastrocnemius muscles and a decrease in muscle activation of the anterior tibialis muscle (p < 0.05). An increase in knee moments and work done and a reduction in hip work were observed in male subjects (p < 0.05). In conclusion, heel raises affect lower extremity kinematics and kinetics during the barbell squat and alter the distribution of muscle activation and biomechanical loading of the joints in the lower extremity of participants to some extent, and there were gender differences in the results.
Exosomes in Alpha-Synucleinopathies: Propagators of Pathology or Potential Candidates for Nanotherapeutics?
Authors: Mavroeidi Panagiota , Vetsi Maria , Dionysopoulou Dimitra , Xilouri Maria
Published: July 2022

The pathological accumulation of alpha-synuclein governs the pathogenesis of neurodegenerative disorders, such as Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy, collectively termed alpha-synucleinopathies. Alpha-synuclein can be released in the extracellular space, partly via exosomes, and this extracellular protein pool may contribute to disease progression by facilitating the spread of pathological alpha-synuclein or activating immune cells. The content of exosomes depends on their origin and includes specific proteins, lipids, functional mRNAs and various non-coding RNAs. Given their ability to mediate intercellular communication via the transport of multilevel information, exosomes are considered to be transporters of toxic agents. Beyond neurons, glial cells also release exosomes, which may contain inflammatory molecules and this glia-to-neuron or neuron-to-glia transmission of exosomal alpha-synuclein may contribute to the propagation of pathology and neuroinflammation throughout the brain. In addition, as their content varies as per their originating and recipient cells, these vesicles can be utilized as a diagnostic biomarker for early disease detection, whereas targeted exosomes may be used as scaffolds to deliver therapeutic agents into the brain. This review summarizes the current knowledge regarding the role of exosomes in the progression of alpha-synuclein-related pathology and their potential use as biomarkers and nanotherapeutics in alpha-synucleinopathies.
Metabotropic Glutamate Receptor 5 in the Dysgranular Zone of Primary Somatosensory Cortex Mediates Neuropathic Pain in Rats
Authors: Chung Geehoon , Yun Yeong-Chan , Kim Chae Young , Kim Sun Kwang , Kim Sang Jeong
Published: July 2022

The primary somatosensory cortex (S1) plays a key role in the discrimination of somatic sensations. Among subdivisions in S1, the dysgranular zone of rodent S1 (S1DZ) is homologous to Brodmann’s area 3a of primate S1, which is involved in the processing of noxious signals from the body. However, molecular changes in this region and their role in the pathological pain state have never been studied. In this study, we identified molecular alteration of the S1DZ in a rat model of neuropathic pain induced by right L5 spinal nerve ligation (SNL) surgery and investigated its functional role in pain symptoms. Brain images acquired from SNL group and control group in our previous study were analyzed, and behaviors were measured using the von Frey test, acetone test, and conditioned place preference test. We found that metabotropic glutamate receptor 5 (mGluR5) levels were significantly upregulated in the S1DZ contralateral to the nerve injury in the SNL group compared to the sham group. Pharmacological deactivation of mGluR5 in S1DZ ameliorated symptoms of neuropathic allodynia, which was shown by a significant increase in the mechanical paw withdrawal threshold and a decrease in the behavioral response to cold stimuli. We further confirmed that this treatment induced relief from the tonic-aversive state of chronic neuropathic pain, as a place preference memory associated with the treatment-paired chamber was formed in rats with neuropathic pain. Our data provide evidence that mGluR5 in the S1DZ is involved in the manifestation of abnormal pain sensations in the neuropathic pain state.