bluuebunny/crossref_metadata_2025 huggingface dataset:
Стан лісових ділянок Житомирського ОУЛМГ, пошкоджених внаслідок видобутку бурштину
Authors: Kovalevskii Sergii , Marchuk Yuri , Maevsky Konstantin , Kurdyuk Olexander , Kovalevskyi Sergii
Published: November 2018

Оцінено стан лісових ділянок Житомирського ОУЛМГ, які зазнали пошкоджень внаслідок незаконного видобутку бурштину. За результатами аналізу встановлено найпоширеніші типи лісорослинних умов і типи лісу, які зазнали пошкоджень. З’ясовано, що внаслідок незаконного видобутку пошкоджено та знищено низку територій, що мають статус заказників місцевого та загальнодержавного значення. Наведено узагальнені дані про географію сучасного розміщення покладів бурштину на території Українського Полісся. Зазначено передумови виникнення проблеми незаконного видобутку бурштину. Наведено перелік територій, де активність самовільних старателів є найбільшою. Визначено основні екологічні, економічні та соціальні наслідки незаконного видобутку бурштину, описано специфіку ушкоджень. Встановлено, що питання рекультивації та, зокрема дендрорекультивації лісових земель, порушених унаслідок несанкціонованого видобутку бурштину на території Українського Полісся, є новим і не має аналогів у світі. Перші наукові публікації з цього питання почали з’являтися лише  три роки тому, їхніми основними напрямами є супутниковий моніторинг порушених територій та оцінювання екологічних наслідків і економічних збитків, завданих несанкціонованим видобутком бурштину. Необхідними передумовами для ефективних заходів з рекультивації порушених територій є: об’єктивна оцінка масштабів та наслідків порушень; здійснення аналізу основних типів порушень ґрунту та лісових ценозів; встановлення особливостей та стимулювання природного відновлення рослинності на деградованих територіях; дослідження впливу абіотичних екологічних чинників на процеси поновлення рослинного покриву.
A perceção dos profissionais de saúde sobre fontes de informação e sua influência na relação terapêutica
Authors: Vaz de Almeida Cristina , Sebastião Sonia Pedro
Published: November 2018

Este estudo tem como objetivo a aferição da perceção dos profissionais de saúde sobre a importância das fontes de informação para a relação terapêutica. Recorreu-se a uma metodologia qualitativa, através da realização de focus group, baseado num questionário semiestruturado, aplicado a nove especialistas em literacia em saúde e profissionais das áreas da saúde em Portugal. Os participantes enfatizaram a responsabilidade do profissional de saúde como fonte especialista e credível para o fornecimento de informação e direcionamento do paciente quando o mesmo recorre a fontes menos credíveis como a internet. Foi ainda destacado o papel dos pares (outros profissionais de saúde) e dos pacientes que prestam depoimento sobre a sua experiência (por exemplo, doentes crónicos).
Book Review - De velhas e novas humanidades, velhos e novos estudos de média: uma leitura crítica de Blow up the humanities e Greening the media
Authors: Sampaio Sofia
Published: November 2018

O artigo faz uma leitura crítica dos livros Blow up the humanities, de Toby Miller, e Greening the media, de Richard Maxwell e Toby Miller, ambos de 2012, que, a pretexto da análise de duas “crises” – a crise das humanidades e a crise ambiental (ou “eco-crise”) – propõem uma renovação das humanidades e dos estudos dos média em linha com a matriz materialista dos estudos culturais. Em ambos os casos, defende-se o alargamento radical dos objectos de estudo e das abordagens, num quadro de “robusta interdisciplinaridade” que combina métodos quantitativos e qualitativos e que valoriza o trabalho académico colectivo e colaborativo.
La importancia de la audiencia en diferido frente a la audiencia lineal en la televisión en España
Authors: Gallardo-Camacho Jorge , Lavín Eva
Published: November 2018

Esta investigación intenta cuantificar la importancia de la audiencia en diferido frente a la audiencia lineal o tradicional en el mercado televisivo en España desde una nueva perspectiva basada en la audiencia acumulada de los programas analizados. Planteamos tres hipótesis de investigación: la H1 asegura que el peso de la audiencia en diferido en España continúa siendo muy reducido cuantitativamente frente a la audiencia tradicional; la H2 determina que los géneros con mayor número de telespectadores acumulados en diferido son los vinculados a la ficción frente a los de actualidad y entretenimiento; y la H3 afirma que las series españolas generan más audiencia en diferido que las extranjeras. Para comprobar las hipótesis proponemos una metodología basada en el estudio de los 10 programas más vistos en diferido durante nueve meses pero, en este caso, nos centraremos en la audiencia acumulada de los programas y no en su frecuencia. Concluiremos que la audiencia en diferido tiene más peso de lo que pensábamos porque ya está transformando, en algunos casos, la manera en la que se determina si un programa o no ha tenido éxito. También concluiremos que los géneros de ficción son los que obtienen mayor audiencia en diferido y, primordialmente, las series españolas por el interés que el contenido local también genera en Internet.
Autism and Social Media: The Case of Brazil
Authors: Antunes Débora , Dhoest Alexander
Published: November 2018

This paper presents an overview of how social media is used by Brazilian autism communities, aiming at including the perspectives of the Global South into discussions surrounding the topic, which are mostly focusing on English speaking groups. The results are based on a digital ethnography conducted in three Facebook groups about autism. We observed that groups composed by parents looking for information and social support are predominant in Brazilian social media. Autistic people also engage in information gathering and the exchange of personal experiences, mainly the ones with Asperger Syndrome. Additionally, we saw that Facebook offers enough affordances to allow the participation of people with different levels of digital literacy. However, there is still a lack of representation from autistic people who have more challenges, such as non-verbal individuals, which may be a consequence of the digital divide, not only in terms of economical means, but also education and inclusion.
La fotografía en Twitter como forma de construcción de imagen política. El caso de las elecciones generales de 2015 en España
Authors: Bustos Díaz Javier , Ruiz del Olmo Francisco Javier
Published: November 2018

El objeto de este trabajo es analizar los aspectos visuales de los perfiles oficiales en Twitter de los principales candidatos a las elecciones generales de España de 2015. Para ello se emplea una metodología cualitativa a través de un sistema de categorías aplicado a la muestra del estudio, conformada por 527 imágenes. Esta investigación concluye que la gestión de las imágenes realizada por los perfiles de los principales candidatos políticos, que concurren a las elecciones generales, se centra sobre todo en la propia figura del político, dejando a un lado aspectos fundamentales como la exposición de propuestas y nuevas iniciativas para la ciudadanía.
TV news and social audience in Europe (EU5): On-screen and Twitter Strategies
Authors: Delgado Matilde , Navarro Celina , Garcia-Muñoz Nuria , LLuís Pau , Paz Elisa
Published: November 2018

Social networks have altered how viewers watch television and consume information. This new context has obliged broadcasters to adapt their practices to reach viewers in all platforms. In this article, we focus on how the evening news programmes from the general-interest DTT channels in the five main European markets (France, Germany, Italy, Spain, and the United Kingdom) appeal to their social audience. This concept, although ambiguous, refers to the activity of viewers on social networks in relation to the contents broadcast by the television industry in its multiple forms. We analyse both the screen strategies and their activity on their official Twitter accounts. Although the evening news is the anchor of the prime time and one of the main assets to instil the channels with prestige and influence, the conclusions point to a certain neglect of the social audience from the industry in these key programmes. But mostly, our research reveals how the specificities of the format and the nature of the content distinguish the evening news from other live TV shows.
Alite calcium sulfoaluminate cement: chemistry and thermodynamics
Authors: Hanein Theodore , Duvallet Tristana Y. , Jewell Robert B. , Oberlink Anne E. , Robl Thomas L. , Zhou Yongmin , Glasser Fredrik P. , Bannerman Marcus N.
Published: November 2018

Calcium sulfoaluminate (C$A) cement is a binder of increasing interest to the cement industry and is undergoing rapid development. Current formulations do not contain alite; however, alite calcium sulfoaluminate (a-C$A) cements can combine the favourable characteristics of Portland cement (PC) with those of C$A cement while also having a lower carbon dioxide footprint than the current generation of PC clinkers. This paper presents two results on the formation of a-C$A clinkers. The first is a thermodynamic study demonstrating that the production of a-C$A clinker is possible without the use of mineralisers, doping with foreign elements, or using multiple stages of heating. It is established that a-C$A clinker can be readily produced in a standard process by controlling the oxygen and sulfur dioxide fugacity in the atmosphere. This allows for the stabilisation of ye’elimite to the higher temperatures required for alite stability. The second result establishes that when using fluorine to mineralise a-C$A clinker production, the iron content in the clinker is also an important variable. Although the exact mechanism of alite stabilisation is not known, it is shown that alite formation increases with the combination of calcium fluoride and iron (III) oxide in the mix.
Bioinspired multifunctional layered magnetic hybrid materials
Authors: Debus Christian , Wu Baohu , Kollmann Tina , Duchstein Patrick , Siglreitmeier Maria , Herrera Steven , Benke Dominik , Kisailus David , Schwahn Dietmar , Pipich Vitaliy , Faivre Damien , Zahn Dirk , Cölfen Helmut
Published: November 2018

Nature has taken millennia to come up with unique solutions for providing materials with properties tailored toward versatile demands, making use of the very limited resources available in natural environments. Today, these biomaterials can be used as inspiration by combining and ‘remixing’ the concepts that nature displays to create new bioinspired materials. Here, the authors present materials combining the structural and functional elements of multiple biominerals: the inorganic–organic lamellar structure responsible for the high fracture toughness of nacre; highly mineralized composites, which give different mollusk teeth their very high hardness and strength; and the particle orientation and magnetic anisotropy of magnetosomes, giving magnetotactic bacteria a sensitive means to navigate along geomagnetic field lines. The authors show how the mechanical properties of a composite material can be improved with the addition of each of these elements. Small-angle neutron scattering studies and molecular simulation give additional insights into the mineralization from the very first attached ions to the finished composite.
Feasibility of recovered toner powder as an integral pigment in concrete
Authors: Newlands Moray , Csetenyi Laszlo , Zheng Li
Published: November 2018

Colour is an important property in many construction materials with pigments, coatings and paints being used primarily for aesthetic, safety and restoration purposes. However, the use of integral pigments in materials such as mortar and concrete can significantly increase material costs. Recovered toner powder (RTP) from printer and photocopier cartridges has the potential to be a low-cost and sustainable alternative pigment. The aim of this research was to examine the feasibility of using cyan, yellow, magenta and black RTP to create a range of colour options for mortar and concrete, and thereafter assess the colour stability in outdoor, indoor, ultraviolet and wet/dry conditions using the colour change parameter ΔE. The work showed that the RTP as a pigment could be blended to make a range of primary and secondary colours and had good colour stability in all environments with minimal impact on selected properties of hardened concrete.
Behaviour of hybrid fibre-reinforced engineered cementitious composites with strain recovery
Authors: Ali Mohamed A. E. M. , Nehdi Moncef L.
Published: November 2018

A self-centring engineered cementitious composite (ECC) was developed. The novel ECC incorporates a hybrid combination of short and dispersed polyvinyl alcohol and shape memory alloy (SMA) fibres, thus achieving superior tensile and flexural characteristics. In addition to its enhanced ductility, the composite is endowed with strain recovery capability, which could be achieved by heat treatment, leading to crack closing through the shape memory effect of the SMA fibres. An inverse analysis method was proposed based on the experimental results to predict the uniaxial tensile test results of the ECC composites using simple flexural test results. The inverse method proved to be an effective predictive tool, cost effective in terms of laborious direct tensile tests and the associated sophisticated test set-up. The ductile composite endowed with crack-closing capability is a strong contender for critical infrastructure projects and protective structures.
Ultralightweight foundation system for peaty ground
Authors: Aminu Ibrahim , Asadi Afshin , O’Kelly Brendan C , Huat Bujang B K , Reul Oliver
Published: November 2018

Construction on peat deposits represents a major challenge for the geotechnical community. Waterlogged peat deposits have great potential for buoyancy generation. The premise of the present investigation is that this can be beneficially incorporated in foundation design practice, thereby reducing the net bearing pressure and hence resulting settlements. A novel foundation system, comprising a bamboo frame (BF) structure incorporating recycled plastic block (RPB) inclusions, is presented for supporting lightweight structures bearing on peaty ground. The buoyancy effect is produced by the lower bulk density of the foundation construction materials combined with the waterlogged condition of the peat deposit. A programme of reduced-scale 1 g physical modelling was conducted to investigate the performance of BF- and BF–RPB-type footing bearing on remoulded peat with different water content and fibre content (FC) values. The mobilised undrained bearing capacity (q f) increased for lower-water-content and higher-FC peat materials. Deeper BF footings and the inclusion of the RPBs within their cavities significantly improved the mobilised q f value. Advantages of the presented foundation system over conventional solutions for peaty ground include its simple technology, reduced earthworks in construction, reduced settlement due to the buoyancy contribution and being more sustainable and economically viable.
Hydro-mechanical behaviour of high-density bentonite pellet on partial hydration
Authors: Darde B. , Tang A. M. , Pereira J.-M. , Roux J.-N. , Dangla P. , Talandier J. , Vu M. N.
Published: November 2018

The hydro-mechanical behaviour of a high-density bentonite pellet, potential candidate for engineered barriers in high-level radioactive waste disposal, is investigated through laboratory tests. Water content and volumetric strain are first determined at various suctions (ranging from 9 to 89 MPa) during partial hydration from its initial state. Afterwards, compression tests allow Young's modulus and strength to be determined at various suctions. The experimental results are then interpreted by using an existing model describing the hydro-mechanical behaviour of an aggregate in compacted expansive clay. The analyses show that a single set of parameters is sufficient to predict the suction dependency of volumetric strain, Young's modulus and compressive strengths. These findings would be helpful for further numerical investigations on the hydro-mechanical behaviour of granular bentonite-based engineered barriers by using both finite- and discrete-element methods.
Determination of optimum rock cutting data through single pick cutting tests
Authors: Yasar S.
Published: November 2018

Partial-face rock excavation machines, which are equipped with conical picks form a major share in the mineral production and in the roadway excavation for underground mines. Consequently, the performance of these machines plays a key role for the success of the project where the prediction of its performance generally relies on the instrumented cutting tests requiring a large rock sample. These rock samples, however, are in most cases impossible to be collected from excavation sites, especially from underground mines due to safety issues and harsh working environment. Therefore, alternative reliable methods requiring a smaller amount of rock sample are preferred. This paper was tailored to address a new plot to convert the index cutting data from the core cutting test into the optimum rock cutting data where these data are used for performance prediction and machine design. A special emphasis was given to medium strength rocks in which partial-face rock excavation machines frequently operate. Nine rock samples were collected from quarries and roadheader excavation sites, which have a strength spectrum from 1·89 to 99·92 MPa and these samples were subjected to rock cutting tests both using the procedure for the core-cutting test in single pick cutting mode and for the conical pick cutting test in relieved mode. In virtue of cutting tests, predictive plots were developed to determine the optimum conical pick cutting data through conducting a single pick cutting test using a small block sample. The major conclusion drawn from the experimental results is in the absence of large rock sample for an instrumented cutting test, small block or core samples can be utilised for estimation of optimum rock cutting data, especially for very low and medium strength rocks.
Vibration propagation of subgrade built on treated dredged fill
Authors: Ding Guangya , Sun Fan , Dai Pengyan
Published: November 2018

Saturated soft clay subgrade in Wenzhou was reinforced by a combination of vacuum preloading and surcharge preloading using different loading materials. Using the principle of similarity, embankments were erected on samples of the soft clay subgrade in a large model box using three different materials (slag, coarse aggregate and sand). A vibration transmission test was conducted on the samples. The test results were used to analyse three factors that majorly affect the transmission of dynamic loads. The relationship between the vibration velocity and the distance from the vibration source was also investigated and used to derive a modified vibration attenuation equation. An increase in subgrade compressive stiffness was found to be helpful to decrease the vibration amplitude. In addition, the attenuation of the vertical vibration velocity was observed to accelerate with increasing embankment height and width.
Influence of nanosilica on mechanical and durability properties of concrete
Authors: Kumar Suman , Kumar Arun , Kujur Jitu
Published: November 2018

The results of an experimental study on the effect of the partial replacement of ordinary Portland cement with nanosilica (NS) on the mechanical and durability properties of concrete are presented. Concrete mixes were made with varying NS contents at a constant water/binder ratio of 0·40. The test results demonstrated that the inclusion of 3% NS as a partial replacement of cement enhanced the mechanical properties and reduced the water penetration depth and water absorption of the concrete, resulting in overall improved performance. Compared with normal concrete, the water penetration depth and water absorption of the NS concrete was reduced by 42·85% and 36·84%, respectively. The carbonation depth was also reduced as a result of cement replacement with NS, leading to enhanced concrete durability. These enhancements in the mechanical and durability properties were mainly due to the high fineness and increased pozzolanic activity of the added NS.
Effect of soil–structure interaction on a masonry structure under train-induced vibrations
Authors: Khan Bilal Lal , Farooq Hassan , Usman Muhammad , Butt Faheem , Khan Abdul Qudoos , Hanif Asad
Published: November 2018

This paper presents an experimental investigation to assess the influence of soil–structure interaction (SSI) on an unreinforced masonry structure under train-induced vibrations. For this purpose, a structure near a railway line was instrumented and monitored when subjected to five railway traffic vibrations. The measured vibrations were used to estimate the modal properties using a frequency domain decomposition technique. The variation in estimated modal properties against varying excitations indicated that the dynamic response depends on the source of excitation. Two finite-element (FE) models were also developed and updated through manual tuning, one with a fixed base and the other with a flexible base accounting for SSI. The modal properties and response time histories measured through experimentation were compared to those predicted by detailed three-dimensional FE models. A comparison between the base input force, base moment and peak displacement of both FE models was also performed. The results indicated that the effect of SSI on the fundamental mode shape and in the prediction of accurate response time histories of the investigated structure was significant. However, the effect on modal frequencies, base input force, base moment and peak displacement of the investigated structure under train-induced vibrations was observed to be very low.
Follower behavioural change based on natural patterns in traffic oscillation
Authors: Salehikalam Arsalan , Kordani Ali Abdi
Published: November 2018

An understanding of the change in follower behaviour as a driver characteristic in traffic simulations is necessary because these behavioural changes are frequently observed in traffic oscillations. The change in follower behaviour due to traffic acceleration and deceleration waves can be classified according to different behavioural patterns resulting from different driver reactions. Combinations of existing behavioural patterns (under-reaction–timid, over-reaction–timid and constant over-reaction–timid patterns) were thus analysed. The method of analysis was based on an artificial neural network model at the microscopic level in order to simulate the extent to which follower behaviour deviates from Newell's car-following model. The most influential parameters of all the behavioural patterns were determined. Based on the under-reaction–timid pattern, decreasing the reaction speed resulted in a change in behaviour due to the development of safe spacing at the reaction point. In addition, follower spacing was the most influential in the over-reaction–timid pattern, resulting in increasing and then decreasing behavioural change. In the constant over-reaction–timid pattern, the follower's speed resulted in increasing and then decreasing the change in behaviour. The application of behavioural change to an intelligent transportation system would improve safety.
The validity of canadian cervical spine rules and the NEXUS low risk criteria in trauma patients
Authors: KAYA Bora
Published: November 2018

Background: The
first decision rule developed to identify clinically significant injuries in
blunt cervical trauma patients is National Emergency X-Radiography Utilisation Study (NEXUS). In the NEXUS study, the negative predictive value (NPV) has
been determined as 99.8%. Sensitivity of Canadian
Cervical
Spine Rules (CCR) was reported as
99.4%, specificity as 45.1% and NPV was reported as 100%. The
objective of this study is to determine the
reliability and utility of NEXUS and CCR for Turkish patient population that
has a risk of cervical injury.  Methods:
This prospective observational study
included 225 patients, all stable,
conscious patients over 16 years of age who had acute
trauma and were brought to the hospital with ambulances or using their own
means and who had been injured by a mechanism that may cause cervical trauma, and without exclusion criteria. The patients included in
the study were then evaluated for NEXUS and CCR validity.Results:
When CCR was evaluated as a
whole, it was determined that all pathological cases were identified using
these rules. In terms of identifying the presence of pathological imaging
finding the sensitivity of CCR was 100% (95% CI %
56-100) and specificity was 3.2% (95% CI 1.4-6.7%). NEXUS's sensitivity was
calculated as 93% (95% CI 83-97) and specificity as 1.3%(95% CI 0.2-5.1).

Conclusion: CCR
and the NEXUS were determined to be useful in the emergency department for the exclusion of cervical pathologies. CCR were more reliable and useful when compared with the
NEXUS.
Türk Entomoloji biliminin önemli bir kaybı: Prof. Dr. Şeniz KISMALI’nın ardından (19 Aralık 1942 - 14 Kasım 2017)
Authors: TURANLI Ferit
Published: November 2018

Prof. Dr. Şeniz Kısmalı 19 Aralık 1942 yılında Ankara’da doğmuştur. İlk öğrenimini Ankara’da Kızılay İlkokulu’nda tamamlamıştır. Lise eğitimini ise İzmir Kız Lisesi’nde 1959 yılında tamamlamıştır. Üniversite eğitimine Ege Üniversitesi, Ziraat Fakültesi Bitki Koruma Bölümü’nde 1960 yılında başlamıştır. Prof. Dr. Şeniz Kısmalı, bu bölümden 1964 yılında mezun olduktan sonar, açılan asistanlık sınavını başarıyla geçerek 29Ocak 1965 tarihinde mezun olduğu bölümünde göreve başlamıştır. Doktora eğitimini 1972 yılında tamamladıktan sonra, 1979 ve 1988 yıllarında sırasıyla “Doçent” ve “Profesör” lüğe atanmıştır. Prof. Dr. Şeniz KISMALI “Fakülte ve Fakülte Yönetim Kurulu Üyeliği”, “Bitki Koruma Bölüm Başkanlığı” ve “Türkiye Entomoloji Derneği Başkanlığı” görevlerini yürütmüştür. Türkiye’de ilk kez düzenlenen “VIII Avrupa Entomoloji Kongresi” “Organizasyon Komitesi Başkanlığı”nı yürütmüştür. 19 Aralık 2009 tarihinde emekli olan Prof. Dr. Şeniz KISMALI 14 Kasım 2017 tarihinde vefat etmiştir. Bu yazıda kendisinin akademik ve yönetimsel aktivitelerinden özet olarak bahsedilmiştir. Kendisine Allah’tan rahmet diliyorum.
ChroSpin - ProteinG v1
Authors: Frommholz David , Ehl Alexandra , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Antibodies with ChroSpin Columns by DALEX Biotech. Easy and quick small scale antibody purification from various sources and species. Each ChroSpin column has a binding capacity of > 2 mg (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
ChroSpin - ProteinA v1
Authors: Frommholz David , Ehl Alexandra , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Antibodies with ChroSpin Columns by DALEX Biotech. Easy and quick small scale antibody purification from various sources and species. Each ChroSpin column has a binding capacity of > 2 mg (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
ChroDrip - ProteinA v1
Authors: Frommholz David , Ehl Alexandra , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Antibodies with ChroDrip Columns by DALEX Biotech. Easy and quick small scale antibody purification from various sources and species. Each ChroDrip column has a binding capacity of > 10 mg/ml (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
ChroPlate - ProteinG v1
Authors: Ehl Alexandra , Frommholz David , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Antibodies with ChroPlate Filtration Plates by DALEX Biotech. Easy and quick high throughput antibody purification from various sources and species. Each well of the ChroPlate has a binding capacity of > 1 mg (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
RNA extraction from Calotropis procera v1
Authors: R. V. Coêlho Maria , Rivas Rebeca , Pandolfi Valesca , Ribamar C. Ferreira-Neto José , P. Bezerra-Neto João , Maria Benko-Iseppon Ana , G. Santos Mauro
Published: November 2018

A protocol for extracting RNA from Calotropis procera adjusted from the SV Total RNA Isolation System protocol
ChroSpin - ProteinA/G v1
Authors: Frommholz David , Ehl Alexandra , Stefanczyk Nadine
Published: November 2018

Purification Guide for the Isolation of Antibodies with ChroSpin Columns by DALEX Biotech. Easy and quick small scale antibody purification from various sources and species. Each ChroSpin column has a binding capacity of > 2 mg (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
Protocol for DNA Extraction and Quantitative PCR Detection of Verticillium dahliae from Soil v1
Authors: Wheeler David , Scott Jeness , Kam Sung Dung Jeremiah , Allen Johnson Dennis
Published: November 2018

This protocol describes the extraction of DNA from relatively large volumes of soil and subsequent real-time quantitative PCR (qPCR). The qPCR protocol is a modification of the protocol presented by : Wei, F., Fan, R., Dong, H.-T., Shang, W.-J., Xu, X.-M., Zhu, H.-Q., Yang, J.-R., and Hu, X.-P. 2015. Threshold microsclerotial inoculum for cotton Verticillium wilt determined through wet-sieving and real-time quantitative PCR. Phytopathology 105:220-229.
Restorative minimally invasive interventions in patients with colostomy
Authors: Akhmetzyanov F Sh , Valiev N A , Daminov A N , Bikbov B Sh
Published: November 2018

Aim. To show the benefits of performing mini-access restorative phase on the colon in patients with colostomy. Methods. A retrospective analysis of the results of closure stomy operations in 2011-2017 in two emergency cancer departments of Republican Clinical Oncology Center MH RT was conducted. The inclusion criteria of the study were: 1) surgical intervention for acute intestinal obstruction in colorectal cancer performed in RCOC; 2) mandatory removal of the primary tumor during the first surgery; 3) the presence of a functioning intestinal stoma formed during the first surgery; 4) fixation of both stumps in one stoma. The exclusion criteria were refusal to restore the continuity of the colon for medical indications and the patient's refusal to undergo the surgery. Results. The study included 11 males (44 %) and 14 females (56 %). The age interval was 49 to 81 years, of which 12 patients were over 70 years old (48 %), the average age was 67.7 ± 5.4 years. The tumor removed at the first stage was localized in the right parts of the colon in 5 patients (20 %), and in the left in 20 patients (80 %). By the stages of the malignant process, the patients were distributed as follows: stage B - 14 cases (56 %), stage C - 10 (40 %), stage D - 1 (4 %). The average duration of the surgery was 53 ± 14.38 minutes (40 to 123). The postoperative period was 10.8 ± 1.92 days on average (5 to 18). Conclusion. Restoration of intestinal continuity through mini-paracolostomy access is technically rational, as due to the mini-access the patient undergoes minor surgical trauma; the anatomical proximity of the anastomosed bowel stumps excludes the difficulties associated with the search for the distal stump in the traditional method of recovery; the duration of the postoperative hospital stay decreases significantly.
Comparative analysis of clinical effectiveness of α1-adrenoblockers doxazosin, tamsulosin and silodosin in males with chronic non-inflammatory prostatitis
Authors: Lobkarev A O , Khafiz'yanova R Kh , Lobkarev O A
Published: November 2018

Aim. Comparative study of clinical effectiveness of doxazosin, tamsulosin and silodosin as monotherapy for patients with chronic non-inflammatory prostatitis. Methods. Outpatient medical records (OMR) of patients with the diagnosis of chronic non-inflammatory prostatitis who were treated in “The clinic of ambulatory urology” (Kazan) in 2012-2017, were analyzed: all 173 OMR of patients receiving doxazosin 1 mg QD for 30 days as monotherapy; all 150 OMR of patients receiving doxazosin 2 mg per day BID for 30 days as monotherapy; all 54 OMR of patients receiving doxazosin 4 mg per day BID for 30 days as monotherapy; all 77 OMR of patients receiving tamsulosin 0.4 mg QD for 30 days as monotherapy; all 36 OMR of patients receiving silodosin 8 mg QD for 30 days as monotherapy. Based on these OMR the “Clinical index of chronic prostatitis” (CI CP) was analyzed before the start of pharmacotherapy and 30 days after the beginning. According to the clinical index, the discrete sample was ordered containing the values of CI from 0 points (no complaints) to 50 points (maximal score - the most marked symptoms). Results. Before the beginning of the treatment every sample of patients is homogeneous and after 30-day treatment they lose homogeneity and tend to divide into several homogeneous samples. Conclusion. Clinical effectiveness of doxazosin, tamsulosin and silodosin in men with CNP varies in wide range, is poorly predictable, poorly explainable and needs further studies according to the principles of modern evidence-based medicine.
Epidemiological features of acute and chronic hepatitis B in the Kyrgyz Republic over the 20-year period (1997-2017)
Authors: Tobokalova S T , Bekenova D S , Zairova G M , Nurmatov Z Sh , Nazarbaeva Zh N , Aytieva Zh T
Published: November 2018

Aim. To determine epidemiological features of acute and chronic viral hepatitis B in the Kyrgyz Republic over the period 1997-2017. Меthоds. Analysis was performed on data bases of the National accounting forms on treated cases of acute and chronic hepatitis B cumulated by the Medical Information Centre of the Ministry of Health of Kyrgyz Republic for the period of 1997 to 2017 as well as 384 medical records of patients with chronic hepatitis B treated in the Hepatologic Centre “Tsadmir”. Results. In the Kyrgyz Republic among all acute cases of acute viral hepatitides, hepatitis A comprises 60.5 %, hepatitis B - 16.9 %, the ratio of unverified non-A non-C viral hepatitis - 15.2%. With the introduction of HBV vaccination of children (2000) and improvement of infectious control in healthcare settings, a decline of acute HBV incidence is observed - from 26.7 0/0000 to 5.3 0/0000 due to the decrease of acute hepatitis B among children and adolescents to isolated cases, adults from risk groups. The highest incidence of acute hepatitis B is recorded in Osh and Bishkek, in Jalal-Abad and Chuy Regions. This can be explained by that in the southern and northern capitals of the Kyrgyz Republic and in the near lying areas with high population density, access to laboratory and instrumental methods of diagnosis of viral hepatitides is better, along with the higher awareness of its inhabitants regarding the prevention compared to other regions. At the same time an increase of chronic hepatitis B incidence is observed. Predominant modes of transmission are medical-parenteral exposure and household contact (60.9 %), with frequent formation of family clusters (23.8 %). Imperfections were found in the early detection, reporting and accounting of cases of chronic viral hepatitides as well as in examination of hepatitis B virus carriers. Conclusion. The performed analysis of the incidence and prevalence of acute and chronic hepatitis B in the Kyrgyz Republic allowed assessing the dynamics of the epidemiologic situation in the country regarding this disease, revealed shortcomings in the detection and examination of viral hepatitis B carriers, contributed to development of a new guide for epidemiological surveillance of viral hepatitides.
Leonardo da Vinci and the Insulin Experience
Authors: Kalra Sanjay ,   Department of Endocrinology, Bharti Hospital, Karnal, India, Saboo Banshi ,   Department of Diabetology, DiaCare Hospitals, Ahmedabad, India
Published: November 2018

This editorial is based on the philosophy of Leonardo da Vinci, the great maestro, and its relevance to insulin use. We use the basic principles of scientific thought that he practiced to address a major clinical challenge today: the suboptimal insulin experience. The seven cardinal principles of da Vinci: curiosita, dimostrazione, senzazione, sfumato, arte/scienza, corporalita and connessione are applicable to insulin usage as well. This editorial discusses these principles and their concordance with various therapeutic models and frameworks. These include therapeutic patient education, injection technique, diabetes-friendly healthcare, user-friendly delivery devices, the biopsychosocial model, patient-centred care, diabetes therapy by the ear and motivational interviewing. The need to improve insulin literacy and numeracy, teach injection technique with user-friendly delivery devices, and address insulin distress is articulated. The importance of continuing medical education, learning from experience, following a healthy lifestyle and preventing compassion fatigue is discussed.
Ketocrinology
Authors: Kalra Sanjay ,   Department of Endocrinology, Bharti Hospital, Karnal, India, Singla Rajiv ,   Department of Endocrinology, Kalpavriksh Healthcare, New Delhi, India, Rosha Rahul ,   Department of Nutrition, Novique Healthcare, Pune, India, Dhawan Munish ,   Department of Pediatrics, Miri Piri Hospital, Shahabad, India, Priya Gagan ,   Department of Pediatrics, Miri Piri Hospital, Shahabad, India, Priya Gagan ,   Department of Endocrinology, Fortis Hospital, Mohali, India, Dutta Deep ,   Department of Endocrinology, Venkateshwar Hospital, New Delhi, India
Published: November 2018

Ketocrinology is the study of the relationship between ketosis, including ketogenic diet, and endocrine function of the body. This article introduces this novel concept, discusses its importance, lists the reasons for its neglect, and explores the wide clinical spectrum of the subject. The study of ketocrinology should be an integral part of the subject of endocrinology and metabolism. This will facilitate rational and evidence-based prescription of ketogenic diet, in an effective, safe, and well-tolerated manner.
Underlying Mechanisms Between Diabetes Mellitus and Osteoporosis
Authors: Olarte Oscar Rosero ,   Instituto de Osteoporosis de los Llanos, Osteollanos, Regional Hospital, Villavicencio, Colombia, Andrade Mauricio Alvarez ,   Central Military Hospital, Bogotá, Colombia
Published: November 2018

Several studies have linked diabetes mellitus with an increased risk of bone fractures. Patients with type 1 diabetes have lower bone mineral density and patients with type 2 diabetes have an increased risk of fracture despite normal or high bone mineral density, measured by dual energy x-ray absorptiometry. Different mechanisms have been proposed in the pathophysiology of bone disease in diabetic patients. The mechanisms involved include microvasculature changes, advanced glycation end products accumulation, and altered osteoblast–osteoclast function with increased sclerostin, decreased insulin-like growth factor 1 (IGF1), decreased osteocalcin, and other molecular alterations, leading to a decrease in bone turnover and altered bone quality. Other factors, such as increased risk of falls related to diabetic neuropathy and diabetic retinopathy have also been proposed. Different diagnostic methods have been described for the study of bone disease in patients with diabetes, due to the lack of correlation between bone mineral density measured by dual x-ray absorptiometry and the fracture risk in this group of patients. These methods include the evaluation of bone turnover markers, the FRAX score, the trabecular bone score, and the evaluation of microarchitecture with high-resolution peripheral quantitative tomography. This editorial provides a short overview of the underlying mechanisms between diabetes mellitus and osteoporosis, and the diagnostic methods used to evaluate these patients.
Telemedicine Services for Emerging Adults with Type 1 Diabetes
Authors: Ramchandani Neesha ,   New York University Rory Meyers College of Nursing, New York, NY, US
Published: November 2018

Emerging adults with type 1 diabetes (T1D) often find it challenging to manage their diabetes well due to the many competing priorities they are juggling during this time. The majority of emerging adults (72%) are already looking for health information online, so telemedicine may be an avenue to explore with them. Telemedicine services for individuals with T1D are becoming increasingly popular and have been found to be beneficial for those who use them. However, there are very few multi-component telemedicine services available for individuals with T1D, and none of them provide a comprehensive technology-based system. Additionally, while these multi-component T1D telemedicine systems often included emerging adults in the group under study, the average age of the study subjects was usually above the age range of emerging adulthood. This suggests that specific developmental needs of emerging adults are not being addressed by these telemedicine systems, nor are the issues faced by diverse populations. More research needs to be done to address which telemedicine services, if any, would be beneficial for this especially vulnerable population.
A Review of the Relationship Between Chronic Periodontitis and Diabetes
Authors: Winning Lewis ,   Division of Restorative Dentistry & Periodontology, Dublin Dental University Hospital, Trinity College Dublin, Ireland, Linden Gerard J ,   Centre for Public Health, School of Medicine Dentistry and Biomedical Sciences, Queen#x2019;s University Belfast, Belfast, Northern Ireland
Published: November 2018

Chronic periodontitis (CP) is a common inflammatory disease which is characterized by progressive destruction of the tooth supporting structures and ultimately, potential tooth loss. A relationship between CP and diabetes has been recognized for some time. Both diseases share several collective risk factors, however, recent research has provided greater insight into the relationship with evidence emerging for each disease being a co-factor in the pathogenesis of the other. The aim of this review is to examine recent epidemiological evidence for the impact of diabetes on CP, and in the reverse direction, the impact of CP on diabetes. Based on the available evidence to date, it seems likely that the level of metabolic control influences future periodontal disease risk. In the reverse direction, the presence of CP has the potential to adversely affect glycemic control in both diabetes-affected and diabetes-free individuals. CP may potentially be a risk factor for the development of type 2 diabetes in diabetes-free individuals. Interventional studies show that successful treatment of CP may improve metabolic control in people with diabetes in the short term.
Ischemic Heart Disease in Subjects of Young Age: Current State of the Problem. Features of Etiology, Clinical Manifestation and Prognosis
Authors: Andreenko E. Yu. , Yavelov I. S. , Loukianov М. М. , Vernohaeva A. N. , Drapkina O. M. , Boytsov S. A.
Published: November 2018

In addition to conventional risk factors in young patients with ischemic heart disease (IHD) numerous other risk factors including genetics play an important role in its causation. Molecular genetic testing is recommended for the detection of monogenic diseases with a high risk of developing IHD, such as familial hypercholesterolemia. In majority ofyoung patients, the first manifestation of IHD is an acute coronary syndrome. Young patients with IHD more often have normal coronary arteries or single-vessel coronary disease, and in up to 20% of them cause of myocardial ischemia is not related to atherosclerosis. In general, young patients with IHD have better prognosis. However, there are sex differences in IHD outcomes the prognosis of patients with premature IHD and reason for this is still unclear.
Myocardial Strain and Stiffness Parameters as a Novel Target of Antihypertensive Treatment
Authors: Ostroumova O. D. , Kochetkov A. I.
Published: November 2018

In the review, the clinical significance of increased myocardial stiffness and strain impairment in the settings of arterial hypertension is considered. The mechanisms of increasing myocardial stiffness as a part of hypertensive heart disease are presented. Particular attention is paid to the role of the sympathetic nervous system activation as one of the triggers that induce the connective tissue alteration of cardiac interstitium. The possibilities of echocardiography in the early noninvasive detection of myocardial strain abnormalities are discussed. New ultrasound parameters for describing stiffness properties of the heart are presented. From the evidence-based medicine point of view, the prognostic significance of increasing myocardial stiffness as a risk factor of the adverse cardiovascular events, as well as the possibility of its management with different antihypertensive drugs, is considered. Finally, there are presented clinical trials data, indicating high potential of the highly selective ^1-adrenoblocker bisoprolol for of correction myocardial stiffness and strain impairment.
Yolk sac and its effects on fetal prognosis
Authors: Das Sankar Kumar , Roy Priyanka
Published: November 2018

Background: The yolk sac is the first extra embryonic structure that becomes sonographically visible within the gestational sac. It provides nutritional, metabolic, endocrine, immunologic, and hematopoietic functions during organogenesis in embryonic life, before placental circulation is established. Spontaneous abortion rates are significantly elevated where the yolk sac volume falls outside the 5th to 95th percentile. Abnormal morphological appearance of the yolk sac is also suggestive of serious growth disorders of the fetus. The aim is to find out the predictive value of yolk sac on outcome of the pregnancy and any probable relationship of the yolk sac pattern with the gender of the fetus.Methods: The present study was carried out on fifty patients in their first trimester of pregnancy. Patients were divided into their respective gestational age groups and according to the morphology of the yolk sac. Data were compared using Chi square test. p value less than 0.5 was considered significant.Results: Out of the fifty patients, twenty-nine were having regular yolk sac, eleven were with irregular yolk sac and ten were with hyperechoic yolk sac. There was a significant number of fetal demise in the cases with hyperechoic and irregular yolk sac. Also, there were hardly any live births in the few cases in which the yolk sac was either too small or too big.Conclusions: The present study concludes that along with a yolk sac of a given normal range for its size, it should also be of normal morphology for better prognosis of the pregnancy. Also, the increase in the number of female live births needs further studies as to whether there is any abnormality in the Y chromosome causing morphologically abnormal yolk sac and subsequent fetal demise.