bluuebunny/crossref_metadata_2025 huggingface dataset:
Dental Implants with Anti-Biofilm Properties: A Pilot Study for Developing a New Sericin-Based Coating
Authors: Ghensi Paolo , Bettio Elia , Maniglio Devid , Bonomi Emiliana , Piccoli Federico , Gross Silvia , Caciagli Patrizio , Segata Nicola , Nollo Giandomenico , Tessarolo Francesco
Published: July 2019

Aim: several strategies have been tested in recent years to prevent bacterial colonization of dental implants. Sericin, one of the two main silk proteins, possesses relevant biological activities and also literature reports about its potential antibacterial properties, but results are discordant and not yet definitive. The aim of this study was to evaluate the effectiveness of different experimental protocols in order to obtain a sericin-based coating on medical grade titanium (Ti) able to reduce microbial adhesion to the dental implant surface. Materials and Methods: different strategies for covalent bonding of sericin to Ti were pursued throughout a multi-step procedure on Ti-6Al-4V disks. The surface of grade 5 Ti was initially immersed in NaOH solution to obtain the exposure of functional -OH groups. Two different silanization strategies were then tested using aminopropyltriethoxysilane (APTES). Eventually, the bonding between silanized Ti-6Al-4V and sericin was obtained with two different crosslinking processes: glutaraldehyde (GLU) or carbodiimide/N-Hydroxy-succinimide (EDC/NHS). Micro-morphological and compositional analyses were performed on the samples at each intermediate step to assess the most effective coating strategy able to optimize the silanization and bioconjugation processes. Microbiological tests on the coated Ti-6Al-4V disks were conducted in vitro using a standard biofilm producer strain of Staphylococcus aureus (ATCC 6538) to quantify the inhibition of microbial biofilm formation (anti-biofilm efficacy) at 24 hours. Results: both silanization techniques resulted in a significant increase of silicon (Si) on the Ti-6Al-4V surfaces etched with NaOH. Differences were found between GLU and EDC/NHS bioconjugation strategies in terms of composition, surface micro-morphology and anti-biofilm efficacy. Ti-6Al-4V samples coated with GLU-bound sericin after silanization obtained via vapor phase deposition proved that this technique is the most convenient and effective coating strategy, resulting in a bacterial inhibition of about 53% in respect to the uncoated Ti-6Al-4V disks. Conclusions: The coating with glutaraldehyde-bound sericin after silanization in the vapor phase showed promising bacterial inhibition values with a significant reduction of S. aureus biofilm. Further studies including higher number of replicates and more peri-implant-relevant microorganisms are needed to evaluate the applicability of this experimental protocol to dental implants.
Structure of Non-Oscillatory Solutions for Second Order Dynamic Equations on Time Scales
Authors: Zhou Yong , Ahmad Bashir , Alsaedi Ahmed
Published: July 2019

In this paper, we make a detailed analysis of the structure of non-oscillatory solutions for second order superlinear and sublinear dynamic equations on time scales. The sufficient and necessary conditions for existence of non-oscillatory solutions are presented.
Mitigation of Radiation-Induced Lung Pneumonitis and Fibrosis Using Metformin and Melatonin: A Histopathological Study
Authors: Farhood Bagher , Aliasgharzadeh Akbar , Amini Peyman , Rezaeyan Abolhasan , Tavassoli Alireza , Motevaseli Elahe , Shabeeb Dheyauldeen , Eleojo Musa Ahmed , Najafi Masoud
Published: July 2019

Background and objectives: Pneumonitis and fibrosis are the most common consequences of lung exposure to a high dose of ionizing radiation during an accidental radiological or nuclear event, and may lead to death, after some months to years. So far, some anti-inflammatory and antioxidant agents have been used for mitigation of lung injury. In the present study, we aimed to detect possible mitigatory effects of melatonin and metformin on radiation-induced pneumonitis and lung fibrosis. Materials and methods: 40 male mice were divided into 4 groups (10 mice in each). For control group, mice did not receive radiation or drugs. In group 2, mice were irradiated to chest area with 18 Gy gamma rays. In groups 3 and 4, mice were first irradiated similar to group 2. After 24 h, treatment with melatonin as well as metformin began. Mice were sacrificed after 100 days for determination of mitigation of lung pneumonitis and fibrosis by melatonin or metformin. Results: Results showed that both melatonin and metformin are able to mitigate pneumonitis and fibrosis markers such as infiltration of inflammatory cells, edema, vascular and alveolar thickening, as well as collagen deposition. Conclusion: Melatonin and metformin may have some interesting properties for mitigation of radiation pneumonitis and fibrosis after an accidental radiation event.
The Effect of a Low-Cost Body Weight-Supported Treadmill Trainer on Walking Speed and Joint Motion
Authors: Ventura Jessica D. , Charrette Ann L. , Roberts Katherine J.
Published: July 2019

Background and Objectives: Gait training with body weight-support has been shown to improve the walking speed of individuals with movement disorders. The AccesSportAmerica Gait Trainer is a low-cost, pre-market gait rehabilitation device that alters the stride characteristics of participants walking on a standard treadmill. The purpose of this study was to examine the biomechanical outcomes that training on this device has for people with brain injuries that affect motor functioning. It was hypothesized that there would be an increase in walking speed post-intervention, and that there would be an increase in step length and joint range-of-motion. Materials and Methods: An intervention study was conducted with 11 people with ambulatory difficulty caused by post-stroke hemiparesis (n = 7), traumatic brain injury (n = 3), and cerebral palsy (n = 1). The average time using the AccesSportAmerica Gait Trainer was 34.5 (SD = 6.0) minutes per session for 36.9 (SD = 21.8) sessions. Gait speed, step length and time, and joint flexion were measured during the 10 Meter Walk Test. Results: From pre- to post-intervention, there was a mean increase in walking speed of 0.19 m/s (SD = 0.06, p = 0.016, d = 0.40) and a decrease in step time of both affected and unaffected legs (affected: p = 0.011, d = 0.37; unaffected: p = 0.004, d = 0.67). There was no significant change in stride length or joint angles. Conclusions: The AccesSportAmerica Gait Trainer has the potential to improve the walking speed of people with ambulatory difficulty.
Facile Preparation of CNT/Ag2S Nanocomposites with Improved Visible and NIR Light Photocatalytic Degradation Activity and Their Catalytic Mechanism
Authors: Di Lijing , Xian Tao , Sun Xiaofeng , Li Hongqin , Zhou Yongjie , Ma Jun , Yang Hua
Published: July 2019

In this work, a series of carbon nanotubes (CNT)/Ag2S hybrid nanocomposites were successfully prepared by a facile precipitation method. Transmission electron microscope (TEM) observation indicates that Ag2S nanoparticles with an average particle size of ~25 nm are uniformly anchored on the surface of CNT. The photocatalytic activities of the CNT/Ag2S nanocomposites were investigated toward the degradation of rhodamine B (RhB) under visible and near-infrared (NIR) light irradiation. It is shown that the nanocomposites exhibit obviously enhanced visible and NIR light photocatalytic activities compared with bare Ag2S nanoparticles. Moreover, the recycling photocatalytic experiment demonstrates that the CNT/Ag2S nanocomposites possess excellent photocatalytic stability. The photoelectrochemical and photoluminescence measurements reveal the efficient separation of photogenerated charges in the CNT/Ag2S nanocomposites. This is the dominant reason behind the improvement of the photocatalytic activity. Based on active species trapping experiments, the possible photocatalytic mechanism of CNT/Ag2S nanocomposites for dye degradation under visible and NIR light irradiation was proposed.
Inhibition and Interactions of Campylobacter jejuni from Broiler Chicken Houses with Organic Acids
Authors: Beier Ross , Byrd J. , Caldwell Denise , Andrews Kathleen , Crippen Tawni , Anderson Robin , Nisbet David
Published: July 2019

Campylobacter jejuni is a bacterium that causes major diarrheal disease worldwide and is also one of the top five foodborne pathogens encountered in the United States. Poultry is a major source of C. jejuni, and a high-risk factor for contracting campylobacteriosis. Organic acids are used in the United States during food animal processing for removal of bacterial contamination from animal carcasses. Six organic acids were evaluated in inhibition studies of 96 C. jejuni strains obtained from shoe covers used in broiler chicken houses at different poultry farms in several states by determining the susceptibilities of the C. jejuni strains, along with the pH values at the molar minimum inhibitory concentrations (MICMs). The undissociated and dissociated organic acid concentrations were calculated at the MICMs with the Henderson-Hasselbalch equation. The results for the 96 C. jejuni strains were treated similarly for each different organic acid. Campylobacter jejuni inhibition did correlate with the dissociated organic acids, but did not correlate with pH or with the undissociated organic acids. When the concentrations of dissociated organic acids decreased, the C. jejuni strains were not disinfected. A carcass wash using organic acids should have the concentration of dissociated acid species carefully controlled. It is suggested to maintain a dissociated acid concentration for propionic, l-lactic, formic, citric, butyric, and acetic acids at 24, 40, 36, 21, 23, and 25 mM, respectively, and at these dissociated organic acid levels an acid wash would be expected to remove or inhibit 97% or more of the C. jejuni bacteria studied here. However, studies must be undertaken to confirm that the suggested concentrations of dissociated organic acids are adequate to remove C. jejuni bacteria in the field vs. the laboratory. Due to propionate, l-lactate, formate, butyrate, and acetate being utilized by C. jejuni, these organic acids may not be appropriate for use as a carcass wash to remove C. jejuni surface contamination. Of all tested organic acids, dissociated citric acid was the most efficient at inhibiting C. jejuni.
Strengthening Sulfidation Flotation of Hemimorphite via Pretreatment with Pb2+
Authors: Huang Yuqing , Yin Wanzhong , Deng Rongdong , Xing Dingquan , Rao Feng
Published: July 2019

The conventional sulfidation-xanthate flotation process that consists of sulfidization with sodium sulfide, activation by heavy-metal ions, and collection with xanthate is not sufficiently efficient for treating hemimorphite, and the dosages of the sulfurizing reagent and metal ions are large. In this study, the sulfidation flotation (Pb2+ + Na2S + Pb2+ + xanthate) of hemimorphite was strengthened by pretreating with Pb2+ before sulfidation. Microflotation test results indicated that the recovery of hemimorphite increased by 5–10% after pretreatment with Pb2+. The comprehensive results of adsorption experiments, scanning electron microscopy–energy-dispersive X-ray spectroscopy, atomic force microscopy, and X-ray photoelectron spectroscopy indicated that a large amount of Pb2+ was adsorbed on the hemimorphite surface and entered the lattice, forming Zn(4−x)PbxSi2O7(OH)2·H2O. The newly formed component had an increased amount of surface sulfidation active sites and had the effect of induced crystallization, making the surface more effective for sulfidation. After the Pb2+ was added to the pulp, a large number of uniform and dense PbS species were formed on the hemimorphite surface, increasing the number of adsorption sites for xanthate and reducing the competitive adsorption of residual S2− on the xanthate.
Catalytic Production of Levulinic Acid (LA) from Actual Biomass
Authors: Signoretto Michela , Taghavi Somayeh , Ghedini Elena , Menegazzo Federica
Published: July 2019

Catalytic conversion of actual biomass to valuable chemicals is a crucial issue in green chemistry. This review discusses on the recent approach in the levulinic acid (LA) formation from three prominent generations of biomasses. Our paper highlights the impact of the nature of different types of biomass and their complex structure and impurities, different groups of catalyst, solvents, and reaction system, and condition and all related pros and cons for this process.
Novel Functionalized Cellulose Microspheres for Efficient Separation of Lithium Ion and Its Isotopes: Synthesis and Adsorption Performance
Authors: Chen Ichen , Xu Chenxi , Peng Jing , Han Dong , Liu Siqi , Zhai Maolin
Published: July 2019

The adsorption of lithium ions(Li+) and the separation of lithium isotopes have attracted interests due to their important role in energy storage and nuclear energy, respectively. However, it is still challenging to separate the Li+ and its isotopes with high efficiency and selectivity. A novel cellulose-based microsphere containing crown ethers groups (named as MCM-g-AB15C5) was successfully synthesized by pre-irradiation-induced emulsion grafting of glycidyl methacrylate (GMA) and followed by the chemical reaction between the epoxy group of grafted polymer and 4′-aminobenzo-15-crown-5 (AB15C5). By using MCM-g-AB15C5 as adsorbent, the effects of solvent, metal ions, and adsorption temperature on the adsorption uptake of Li+ and separation factor of 6Li/7Li were investigated in detail. Solvent with low polarity, high adsorption temperature in acetonitrile could improve the uptake of Li+ and separation factor of lithium isotopes. The MCM-g-AB15C5 exhibited the strongest adsorption affinity to Li+ with a separation factor of 1.022 ± 0.002 for 6Li/7Li in acetonitrile. The adsorption isotherms in acetonitrile is fitted well with the Langmuir model with an ultrahigh adsorption capacity up to 12.9 mg·g−1, indicating the unexpected complexation ratio of 1:2 between MCM-g-AB15C5 and Li+. The thermodynamics study confirmed the adsorption process is the endothermic, spontaneous, and chemisorption adsorption. As-prepared novel cellulose-based adsorbents are promising materials for the efficient and selective separation of Li+ and its isotopes.
Thyroid Peroxidase Activity is Inhibited by Phenolic Compounds—Impact of Interaction
Authors: Habza-Kowalska Ewa , Kaczor Agnieszka A. , Żuk Justyna , Matosiuk Dariusz , Gawlik-Dziki Urszula
Published: July 2019

The aim of this study was to estimate the mode of thyroid peroxidase (TPO) inhibition by polyphenols: Chlorogenic acid, rosmarinic acid, quercetin, and rutin. All the tested polyphenols inhibited TPO; the IC50 values ranged from 0.004 mM to 1.44 mM (for rosmarinic acid and rutin, respectively). All these pure phytochemical substances exhibited different modes of TPO inhibition. Rutin and rosmarinic acid showed competitive, quercetin—uncompetitive and chlorogenic acid—noncompetitive inhibition effect on TPO. Homology modeling was used to gain insight into the 3D structure of TPO and molecular docking was applied to study the interactions of the inhibitors with their target at the molecular level. Moreover, the type and strength of mutual interactions between the inhibitors (expressed as the combination index, CI) were analyzed. Slight synergism, antagonism, and moderate antagonism were found in the case of the combined addition of the pure polyphenols. Rutin and quercetin as well as rutin and rosmarinic acid acted additively (CI = 0.096 and 1.06, respectively), while rutin and chlorogenic acid demonstrated slight synergism (CI = 0.88) and rosmarinic acid with quercetin and rosmarinic acid with chlorogenic acid showed moderate antagonism (CI = 1.45 and 1.25, respectively). The mixture of chlorogenic acid and quercetin demonstrated antagonism (CI = 1.79). All the polyphenols showed in vitro antiradical ability against 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), ABTS. The highest ability (expressed as IC50) was exhibited by rosmarinic acid (0.12 mM) and the lowest value was ascribed to quercetin (0.45 mM).
Comparative Analysis of Radical Adduct Formation (RAF) Products and Antioxidant Pathways between Myricetin-3-O-Galactoside and Myricetin Aglycone
Authors: Li Xican , Ouyang Xiaojian , Liang Minshi , Chen Dongfeng
Published: July 2019

The biological process, 3-O-galactosylation, is important in plant cells. To understand the mechanism of the reduction of flavonol antioxidative activity by 3-O-galactosylation, myricetin-3-O-galactoside (M3OGa) and myricetin aglycone were each incubated with 2 mol α,α-diphenyl-β-picrylhydrazyl radical (DPPH•) and subsequently comparatively analyzed for radical adduct formation (RAF) products using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS) technology. The analyses revealed that M3OGa afforded an M3OGa–DPPH adduct (m/z 873.1573) and an M3OGa–M3OGa dimer (m/z 958.1620). Similarly, myricetin yielded a myricetin–DPPH adduct (m/z 711.1039) and a myricetin–myricetin dimer (m/z 634.0544). Subsequently, M3OGa and myricetin were compared using three redox-dependent antioxidant analyses, including DPPH•-trapping analysis, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•)-trapping analysis, and •O2 inhibition analysis. In the three analyses, M3OGa always possessed higher IC50 values than those of myricetin. Conclusively, M3OGa and its myricetin aglycone could trap the free radical via a chain reaction comprising of a propagation step and a termination step. At the propagation step, both M3OGa and myricetin could trap radicals through redox-dependent antioxidant pathways. The 3-O-galactosylation process, however, could limit these pathways; thus, M3OGa is an inferior antioxidant compared to its myricetin aglycone. Nevertheless, 3-O-galactosylation has a negligible effect on the termination step. This 3-O-galactosylation effect has provided novel evidence that the difference in the antioxidative activities of phytophenols exists at the propagation step rather than the termination step.
Protic Ionic Liquids as Efficient Solvents in Microwave-Assisted Extraction of Rhein and Emodin from Rheum palmatum L.
Authors: Fan Yunchang , Niu Zeyu , Xu Chen , Yang Lei , Yang Tuojie
Published: July 2019

Rheum palmatum L. (R. palmatum L.) is a traditional Chinese herb and food, in which rhein and emodin are the main bioactive components. The extraction of the two compounds from R. palmatum L. is, thus, of great importance. In this work, protic ionic liquids (PILs) were applied in the microwave-assisted extraction (MAE) of rhein and emodin from R. palmatum L., which avoids the toxicity of organic solvents. The results of the present study indicate that PILs possessing higher polarity exhibit higher extraction ability due to their stronger absorption ability for microwave irradiation. Compared with conventional solvents, such as methanol, trichloromethane, and deep eutectic solvents (DESs), the PIL, 1-butyl-3-himidazolium methanesulfonate ([BHim]MeSO3) reported herein is more efficient. The selected extraction conditions of liquid–solid ratio, microwave irradiation time, microwave irradiation power, and PIL concentration were 40 g·g−1, 50 s, 280 W, and 80%, respectively. Under the selected conditions, the extraction yields of rhein and emodin were 7.8 and 4.0 mg·g−1, respectively. These results suggest that PILs are efficient extraction solvents for the separation of active components from natural products.
Identification of Patulin from Penicillium coprobium as a Toxin for Enteric Neurons
Authors: Brand Benjamin , Stoye Nicolai M. , dos Santos Guilherme Malena , Nguyen Vu Thu Thuy , Baumgaertner Julia C. , Schüffler Anja , Thines Eckhard , Endres Kristina
Published: July 2019

The identification and characterization of fungal commensals of the human gut (the mycobiota) is ongoing, and the effects of their various secondary metabolites on the health and disease of the host is a matter of current research. While the neurons of the central nervous system might be affected indirectly by compounds from gut microorganisms, the largest peripheral neuronal network (the enteric nervous system) is located within the gut and is exposed directly to such metabolites. We analyzed 320 fungal extracts and their effect on the viability of a human neuronal cell line (SH-SY5Y), as well as their effects on the viability and functionality of the most effective compound on primary enteric neurons of murine origin. An extract from P. coprobium was identified to decrease viability with an EC50 of 0.23 ng/µL in SH-SY5Y cells and an EC50 of 1 ng/µL in enteric neurons. Further spectral analysis revealed that the effective compound was patulin, and that this polyketide lactone is not only capable of evoking ROS production in SH-SY5Y cells, but also diverse functional disabilities in primary enteric neurons such as altered calcium signaling. As patulin can be found as a common contaminant on fruit and vegetables and causes intestinal injury, deciphering its specific impact on enteric neurons might help in the elaboration of preventive strategies.
Preparation of Nanofibrous PVDF Membrane by Solution Blow Spinning for Mechanical Energy Harvesting
Authors: Liu Rui-Qiang , Wang Xiao-Xiong , Fu Jie , Zhang Qian-Qian , Song Wei-Zhi , Xu Yuan , Chen You-Qiang , Ramakrishna Seeram , Long Yun-Ze
Published: July 2019

Self-powered nanogenerators composed of poly(vinylidene fluoride) (PVDF) have received much attention. Solution blow spinning (SBS) is a neoteric process for preparing nanofiber mats with high efficiency and safely, and SBS is a mature fiber-forming technology that offers many advantages over conventional electrospinning methods. Herein, we adopted the SBS method to prepare independent PVDF nanofiber membranes (NFMs), and successfully employed them as nanogenerators. Finally, we tested the change in the output current caused by mechanical compression and stretching, and studied its durability and robustness by charging the capacitor, which can drive tiny electronic devices. The results show that the PVDF nanogenerators by using this SBS equipment can not only be used in wearable electronic textiles, but are also suitable for potential applications in micro-energy harvesting equipment.
Nanophosphor-Based Contrast Agents for Spectral X-ray Imaging
Authors: Smith Kevin , Getzin Matthew , Garfield Josephine J. , Suvarnapathaki Sanika , Camci-Unal Gulden , Wang Ge , Gkikas Manos
Published: July 2019

Lanthanide-based nanophosphors (NPhs) are herein developed as contrast agents for spectral X-ray imaging, highlighting the chemical, macromolecular and structural differences derived from ligand exchange on computed tomography (CT) and solvent dispersibility. Taking advantage of the ability of spectral X-ray imaging with photon-counting detectors to perform image acquisition, analysis, and processing at different energy windows (bins), enhanced signal of our K-edge materials was derived, improving sensitivity of CT imaging, and differentiation between water, tumor-mimic phantoms, and contrast materials. Our results indicate that the most effective of our oleic acid-stabilized K-edge nanoparticles can achieve 2–4x higher contrast than the examined iodinated molecules, making them suitable for deep tissue imaging of tissues or tumors. On the other hand, ligand exchange yielding poly(acrylic acid)-stabilized K-edge nanoparticles allows for high dispersibility and homogeneity in water, but with a lower contrast due to the high density of the polymer grafted, unless further engineering is probed. This is the first well-defined study that manages to correlate NPh grafting density with CT numbers and water dispersibility, laying the groundwork for the development of the next generation CT-guided diagnostic and/or theranostic materials.
The Modern Western Diet Rich in Advanced Glycation End-Products (AGEs): An Overview of Its Impact on Obesity and Early Progression of Renal Pathology
Authors: Bettiga Arianna , Fiorio Francesco , Di Marco Federico , Trevisani Francesco , Romani Annalisa , Porrini Esteban , Salonia Andrea , Montorsi Francesco , Vago Riccardo
Published: July 2019

Advanced glycation end-products (AGEs) are an assorted group of molecules formed through covalent bonds between a reduced sugar and a free amino group of proteins, lipids, and nucleic acids. Glycation alters their structure and function, leading to impaired cell function. They can be originated by physiological processes, when not counterbalanced by detoxification mechanisms, or derive from exogenous sources such as food, cigarette smoke, and air pollution. Their accumulation increases inflammation and oxidative stress through the activation of various mechanisms mainly triggered by binding to their receptors (RAGE). So far, the pathogenic role of AGEs has been evidenced in inflammatory and chronic diseases such as chronic kidney disease, cardiovascular disease, and diabetic nephropathy. This review focuses on the AGE-induced kidney damage, by describing the molecular players involved and investigating its link to the excess of body weight and visceral fat, hallmarks of obesity. Research regarding interventions to reduce AGE accumulation has been of great interest and a nutraceutical approach that would help fighting chronic diseases could be a very useful tool for patients’ everyday lives.
Stability, Continuity, and Bi-Directional Associations of Parental Feeding Practices and Standardized Child Body Mass Index in Children from 2 to 12 Years of Age
Authors: Eichler Janina , Schmidt Ricarda , Poulain Tanja , Hiemisch Andreas , Kiess Wieland , Hilbert Anja
Published: July 2019

(1) Background: Parental feeding practices are related to child body mass index (BMI, kg/m2) and seem to be a consequence rather than cause of child BMI, but research so far is limited. Stability and continuity of feeding practices, probably explaining changes in food intake and child BMI, remain to be poorly examined. (2) Methods: Feeding practices (i.e., restriction, food as reward, pressure to eat, monitoring) assessed via the Child Feeding Questionnaire, child age, standardized BMI (zBMI), and socio-economic status were measured annually at multiple visits (range 2–8) in a population-based longitudinal cohort study of 1512 parents with their children aged 2 to 12 years. Stability, continuity, and bi-directionality of feeding practices and child zBMI were calculated using correlation coefficients, paired t tests, and cross-lagged panels, respectively. (3) Results: Feeding practices and child zBMI showed moderate to high stability. While continuity was high for restriction, minor temporal changes were observed for other feeding practices and child zBMI. Cross-lags indicated that child zBMI predicted restriction, pressure to eat, and monitoring, while food-rewarding predicted child zBMI only minorly. (4) Conclusions: Parents seem to adapt feeding practices to child zBMI with the exception of food-rewarding.
Body Mass Index Z-Score Modifies the Association between Added Sugar Intake and Arterial Stiffness in Youth with Type 1 Diabetes: The Search Nutrition Ancillary Study
Authors: The Natalie S. , Couch Sarah C. , Urbina Elaine M. , Crandell Jamie L. , Liese Angela D. , Dabelea Dana , Kim Grace J. , Tooze Janet A. , Lawrence Jean M. , Mayer-Davis Elizabeth J.
Published: July 2019

The relationship between added sugar and arterial stiffness in youth with type 1 diabetes (T1D) has not been well-described. We used data from the SEARCH for Diabetes in Youth Study (SEARCH), an ongoing observational cohort study, to determine the association between added sugar and arterial stiffness in individuals diagnosed with T1D <20 years of age (n = 1539; mean diabetes duration of 7.9 ± 1.9 years). Added sugar intake was assessed by a food frequency questionnaire, and arterial stiffness measures included pulse wave velocity (PWV) and augmentation index. Separate multivariate linear regression models were used to evaluate the association between added sugar and arterial stiffness. Separate interaction terms were included to test for effect modification by body mass index (BMI) z-score and physical activity (PA). Overall, there was no association between added sugar and arterial stiffness (P > 0.05); however, the association between added sugar and arterial stiffness differed by BMI z-score (P for interaction = 0.003). For participants with lower BMI z-scores, added sugar intake was positively associated with PWV trunk measurements, whereas there was no association for those who had a higher BMI z-score. PA did not significantly modify the association between added sugar and arterial stiffness. Further research is needed to determine the longitudinal relationship and to confirm that obesity differentially affects this association.
The Prevalence of Metabolic Syndrome and Its Related Risk Complications among Koreans
Authors: Lee Seung-Hoo , Tao Shuting , Kim Hak-Seon
Published: July 2019

There is an increasing number of metabolic syndrome (MetS) patients worldwide, and there is no exception in South Korea. The risk complications of metabolic syndrome have been investigated by many previous research studies, while no data on any current trends of MetS are available. Therefore, the present study investigates the recent prevalence of MetS and its associated risk complications in Korean adults by using the Korean National Health and Nutrition Examination Survey (KNHANES). The Survey respondents (n = 4744) were adults over the age of 30, and they had participated in KNHANES 2016, which is a health survey of a national representative sample of non-institutionalized civilian South Koreans. The cross-tabulation analysis was applied to figure out the general characteristics impacting on the prevalence of MetS; furthermore, the odds ratios and 95% confidence intervals (CIs) using multivariate logistic regression analysis were presented for the risk complications of MetS. Findings from this study indicated that subjective health status, family structure, age, income level, use of nutrition labelling and gender showed significant connections with the prevalence of MetS. The risk diseases, stroke (OR = 2.174, 95% CI = 1.377–3.433, p < 0.01), myocardial infarction (MI) (OR = 2.667, 95% CI = 1.474–4.824, p < 0.01) and diabetes (OR = 6.533, 95% CI = 4.963, p < 0.001) were explored and verified attributable to the prevalence of MetS. The findings in this study suggest that sociodemographic characteristics-concentrated strategies are vital to prevent the prevalence of MetS in South Korea, and relative risk complications ought to be cautiously dealt with as well.
Social Jet Lag Associates Negatively with the Adherence to the Mediterranean Diet and Body Mass Index among Young Adults
Authors: Zerón-Rugerio María Fernanda , Cambras Trinitat , Izquierdo-Pulido Maria
Published: July 2019

Obesity and unhealthy eating habits have been associated with irregular sleep–wake patterns during the week, also known as social jet lag. The Mediterranean diet is a healthy pattern related with a better health and sleep quality. However, potential associations with social jet lag remain unexplored. The aim of this study was to examine whether higher social jet lag is linked to lower adherence to the Mediterranean diet and whether it is associated with BMI (Body Mass Index). This cross-sectional study included 534 young adults (18–25 years). Anthropometric parameters, adherence to the Mediterranean diet, chronotype and social jet lag were studied. Our results revealed that individuals with greater social jet lag showed lower adherence to the Mediterranean diet and had a higher BMI. Among the habits that characterized the Mediterranean dietary pattern, we observed that higher social jet lag was significantly associated with a lower intake of fruits and vegetables, as well as skipping breakfast. Hence, the promotion of regular sleep habits together with healthy dietary patterns should be considered for obesity prevention, especially among young adults.
Generation of Coherent and Spatially Squeezed States of an Electromagnetic Beam in a Planar Inhomogeneous Dielectric Waveguide
Authors: Bogatskaya Anna , Schegolev Andrey , Klenov Nikolay , Popov Alexander
Published: July 2019

We use slow-varying amplitude approximation (SVA) for the wave equation to study both analytically and numerically propagation of an electromagnetic beam in the waveguide structure with parabolic susceptibility spatial dependence. Such a structure is similar to the harmonic oscillator in quantum mechanics. We analyze this structure as a single mode guide and introduce the notion of number of “photons” in the mode. In particular, we pay special attention to the possibility of effective build-up of the coherent and spatially squeezed vacuum states of the mode that can be of interest for a number of practical applications. The way to provide these types of mode excitation is suggested. Several applications for controlling the mode composition of an electromagnetic wave in the parabolic index-gradient waveguide for various frequency ranges are considered.
Foliar Application of Zinc Oxide Nanoparticles and Zinc Sulfate Boosts the Content of Bioactive Compounds in Habanero Peppers
Authors: García-López Josué I. , Niño-Medina Guillermo , Olivares-Sáenz Emilio , Lira-Saldivar Ricardo H. , Barriga-Castro Enrique Díaz , Vázquez-Alvarado Rigoberto , Rodríguez-Salinas Pablo A. , Zavala-García Francisco
Published: July 2019

The physiological responses of habanero pepper plants (Capsicum chinense Jacq.) to foliar applications of zinc sulphate and zinc nano-fertilizer were evaluated in greenhouse trials. The effect of the supplement on fruit quality of habanero pepper was particularly observed. Habanero pepper plants were grown to maturity, and during the main stages of phenological development, they were treated with foliar applications of Zn at concentrations of 1000 and 2000 mg L−1 in the form of zinc sulfate (ZnSO4) and zinc oxide nanoparticles (ZnO NPs). Additional Zn was not supplied to the control treatment plants. ZnO NPs at a concentration of 1000 mg L−1 positively affected plant height, stem diameter, and chlorophyll content, and increased fruit yield and biomass accumulation compared to control and ZnSO4 treatments. ZnO NPs at 2000 mg L−1 negatively affected plant growth but significantly increased fruit quality, capsaicin content by 19.3%, dihydrocapsaicin by 10.9%, and Scoville Heat Units by 16.4%. In addition, at 2000 ZnO NPs mg L−1 also increased content of total phenols and total flavonoids (soluble + bound) in fruits (14.50% and 26.9%, respectively), which resulted in higher antioxidant capacity in ABTS (2,2′azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), and FRAP (ferric reducing antioxidant power) (15.4%, 31.8%, and 20.5%, respectively). These results indicate that application of ZnO NPs could be employed in habanero pepper production to improve yield, quality, and nutraceutical properties of fruits.
Biology and Function of miR159 in Plants
Authors: Millar Anthony A. , Lohe Allan , Wong Gigi
Published: July 2019

MicroR159 (miR159) is ancient, being present in the majority of land plants where it targets a class of regulatory genes called GAMYB or GAMYB-like via highly conserved miR159-binding sites. These GAMYB genes encode R2R3 MYB domain transcription factors that transduce the gibberellin (GA) signal in the seed aleurone and the anther tapetum. Here, GAMYB plays a conserved role in promoting the programmed cell death of these tissues, where miR159 function appears weak. By contrast, GAMYB is not involved in GA-signaling in vegetative tissues, but rather its expression is deleterious, leading to the inhibition of growth and development. Here, the major function of miR159 is to mediate strong silencing of GAMYB to enable normal growth. Highlighting this requirement of strong silencing are conserved RNA secondary structures associated with the miR159-binding site in GAMYB mRNA that promotes miR159-mediated repression. Although the miR159-GAMYB pathway in vegetative tissues has been implicated in a number of different functions, presently no conserved role for this pathway has emerged. We will review the current knowledge of the different proposed functions of miR159, and how this ancient pathway has been used as a model to help form our understanding of miRNA biology in plants.
Ethnobotanical Knowledge in Sete Cidades, Azores Archipelago: First Ethnomedicinal Report
Authors: Silva Pedro T. M. , Silva Marta A. F. , Silva Luís , Seca Ana M. L.
Published: July 2019

Knowledge about folk medicines is limited to elder community members of remote communities, like Sete Cidades in the Azores. The Azores, 1300 km west of Portugal, are nine volcanic islands, totalling 2330 km2 of land dispersed by 173,200 km2 in the North Atlantic Ocean. The present study aims to scientifically document the uses of plant species for medicinal purposes, in the Sete Cidades. Twenty-eight community members from 40 to 84 years of age, of whom half were 55 to 64 years old, were interviewed. Twenty-nine taxa were reported as being used for medicinal purposes, ten of which have not been previously reported for ethnomedicinal use in Portugal, with a first record of the use of Morella faya. Leaves were the most used plant part (55%), and decoction the most common preparation mode. The five reported taxa with both the highest use value (0.71–0.25) and relative frequency of citation (0.14–0.11) were Clinopodium menthifolium subsp. ascendens, Aloysia citriodora, Mentha x piperita, Citrus limon and Rosmarinus officinalis. The traditional uses of some of the reported plants are supported by scientific studies, confirming their ethnomedicinal value and the need to preserve local knowledge of folk medicine practices.
Uncovering the Host Range for Maize Pathogen Magnaporthiopsis maydis
Authors: Dor Shlomit , Degani Ofir
Published: July 2019

The fungus Magnaporthiopsis maydis is a soil-borne, seed-borne vascular wilt pathogen that causes severe damage to sensitive Zea mays L. (maize) hybrids throughout Egypt, Israel, India, Spain, and other countries. It can undergo virulence variations and survive as spores, sclerotia, or mycelia on plant residues. Maize, Lupinus termis L. (lupine) and Gossypium hirsutum L. (cotton) are the only known hosts of M. maydis. Identification of new plant hosts that can assist in the survival of the pathogen is an essential step in restricting disease outbreak and spread. Here, by field survey and growth chamber pathogenicity test, accompanied by real-time PCR analysis, the presence of the fungal DNA inside the roots of cotton (Pima cv.) plants was confirmed in infested soil. Moreover, we identified M. maydis in Setaria viridis (green foxtail) and Citrullus lanatus (watermelon, Malali cv.). Infected watermelon sprouts had delayed emergence and development, were shorter, and had reduced root and shoot biomass. M. maydis infection also affected root biomass and phenological development of cotton plants but caused only mild symptoms in green foxtail. No M. maydis DNA was detected in Hordeum vulgare (barley, Noga cv.) and the plants showed no disease symptoms except for reduced shoot weight. These findings are an important step towards uncovering the host range and endophytic behavior of M. maydis, encouraging expanding this evaluation to other plant species.
Mechanical and Morphological Properties of Waterborne ABA Hard-Soft-Hard Block Copolymers Synthesized by Means of RAFT Miniemulsion Polymerization
Authors: Siljanovska Petreska Gordana , Arbe Arantxa , Auschra Clemens , Paulis Maria
Published: July 2019

High molecular weight waterborne ABA block copolymers of styrene (St) and 2-ethylhexyl acrylate (2EHA) containing hard and soft domains were synthesized by means of RAFT (mini)emulsion polymerization using a bifunctional symmetric S,S-dibenzyl trithiocarbonate (DBTTC) RAFT agent. Miniemulsion polymerization was initially used for the synthesis of the A-block, which forms hard domains, followed by 2EHA pre-emulsion feeding to build the B-block soft domains. Polymerization kinetics and the evolution of the Molecular Weight Distribution (MWD) were followed during the synthesis of different ABA block copolymers. The thermal properties of the final symmetric block copolymers were studied on dried films by means of DSC. It was found that the block copolymers have two glass transitions, which indicates the presence of a two-phase system. Phase separation was investigated by means of microscopic techniques (AFM and TEM) and SAXS, both of the particles in the latex form, as well as after film formation at room temperature and after different post-treatments. Films were annealed at temperatures well above the glass transition temperature (Tg) of the hard phase to study the bulk morphology of the films after complete particle coalescence. Moreover, for comparison purposes, the films were re-dissolved in THF, and films were again cast directly from the homogeneous THF solutions. As THF is a good solvent for both blocks, such films serve as a reference for the equilibrium morphology. Finally, DMTA studies of the films annealed at different temperatures were performed to correlate the morphology changes with the mechanical properties of the block copolymers.
The Effect of Extraction Methods on Preliminary Structural Properties and Antioxidant Activities of Polysaccharides from Lactarius vividus
Authors: Xu Zhou , Feng Shiling , Qu Jipeng , Yuan Ming , Yang Ruiwu , Zhou Lijun , Chen Tao , Ding Chunbang
Published: July 2019

Four polysaccharides (LVP-u, LVP-m, LVP-e, and LVP-h) were extracted from the fruiting bodies of Lactarius vividus by an ultrasonic-assisted extraction method, microwave-assisted extraction method, enzyme-assisted extraction method, and hot water extraction method, respectively. Then, the effect of extraction methods on yields, preliminary structural properties, and antioxidant activities was systematically investigated using the weighing method, chemical composition analysis, high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), radical scavenging, and metal ion chelating assays. Results demonstrated that the four L. vividus polysaccharides (LVPs) were all combined with protein, and the yield of LVP-u was higher than others. Molecular weight distribution, monosaccharide and amino acid compositions, and microstructures among the four LVPs were significantly different. Moreover, the LVPs showed significant antioxidant activities in a dose-dependent manner, and LVP-e demonstrated better antioxidant activities in superoxide anion radical scavenging activity assays and metal ion chelating activity assays, while LVP-u showed higher activity in its hydroxyl radical scavenging ability.
An Assessment of Airport Sustainability: Part 3—Water Management at Copenhagen Airport
Authors: Baxter Glenn , Srisaeng Panarat , Wild Graham
Published: July 2019

Sustainable water management is critical for airports as they consume substantial volumes of water to maintain their infrastructure and operations. Airports also generate large volumes of surface and waste waters. The aim of this study was to examine Copenhagen Airport’s sustainable water management strategies and systems from 2006 to 2016. The study used a longitudinal qualitative research design. The annual water consumption at Copenhagen Airport has risen from 2006 to 2016 in line with the increased passenger volumes and aircraft movements. Drinking water is sourced from the Taarnby and Dragør municipal water works. Non-potable water is used wherever possible and is sourced from a local remedial drilling. Copenhagen Airport uses two separate sewer systems for handling surface and wastewater. These waters are not discharged to same system due to their different nature. To mitigate environmental risks and impacts on soil, water, and local communities; the quality of drinking, ground, and surface water are regularly monitored. The airport has implemented various water saving initiatives, such as, an aquifer thermal energy system, to reduce water consumption. The strategies, systems, and the water-saving initiatives have successfully underpinned Copenhagen Airport’s sustainable water management.
Population–Urbanization–Energy Nexus: A Review
Authors: Avtar Ram , Tripathi Saurabh , Aggarwal Ashwani Kumar , Kumar Pankaj
Published: July 2019

Energy expansion and security in the current world scenario focuses on increasing the energy generation capacity and if possible, adopting cleaner and greener energy in that development process. However, too often this expansion and planning alters the landscape and human influence on its surroundings through a very complex mechanism. Resource extraction and land management activity involved in energy infrastructure development and human management of such development systems have long-term and sometimes unforeseen consequences. Although alternative energy sources are being explored, energy production is still highly dependent on fossil fuel, especially in most developing countries. Further, energy production can potentially affect land productivity, land cover, human migration, and other factors involved in running an energy production system, which presents a complex integration of these factors. Thus, land use, energy choices, infrastructure development and the population for which such facilities are being developed must be cognizant of each other, and the interactions between them need to be studied and understood closely. This study strives to analyze the implications of linkages between the energy industry, urbanization, and population and especially highlights processes that can be affected by their interaction. It is found that despite advancement in scientific tools, each of the three components, i.e., population growth, urbanization, and energy production, operates in silos, especially in developing countries, and that this complex issue of nexus is not dealt with in a comprehensive way.
Automatic Detection of Lightning Whistlers Observed by the Plasma Wave Experiment Onboard the Arase Satellite Using the OpenCV Library
Authors: Ali Ahmad Umar , Kasahara Yoshiya , Matsuda Shoya , Ozaki Mitsunori , Goto Yoshitaka
Published: July 2019

The automatic detection of shapes or patterns represented by signals captured from spacecraft data is essential to revealing interesting phenomena. A signal processing approach is generally used to extract useful information from observation data. In this paper, we propose an image analysis approach to process image datasets produced via plasma wave observations by the Arase satellite. The dataset consists of 31,380 PNG files generated from the dynamic power spectra of magnetic wave field data gathered from a one-year observation period from March 2017 to March 2018. We implemented an automatic detection system using image analysis to classify the various types of lightning whistlers according to the Arase whistler map. We successfully detected a large number of whistler traces induced by lightning strikes and recorded their corresponding times and frequencies. The various shapes of the lightning whistlers indicate different very-low-frequency propagations and provide important clues concerning the geospace electron density profile.