bluuebunny/crossref_metadata_2025 huggingface dataset:
Therapeutic Application of Musically Modulated Electromagnetic Fields in the Treatment of Musculoskeletal Disorders
Authors: Corallo C. , Rigato M. , Battisti E. , Albanese A. , Gonnelli S. , Giordano N.
Published: January 2017

Different studies have demonstrated the efficacy of extremely low frequency electromagnetic fields (ELF EMFs) in the treatment of pain. In particular, the positive effects of ELF EMFs seems to depend on their respective codes, such as frequency, intensity and waveform, even if the exact mechanism of interaction is still debated. The most commonly used for extremely low frequency magnetotherapy is a 100Hz sinusoidal field (ELF) with a mean of induction of few Gauss. This article reviews the therapeutic application of a musically modulated electromagnetic field (TAMMEF), a new-generation of electromagnetic field used for extremely low frequency magnetotherapy characterized by variable frequencies, intensities and waveforms. Both clinical and experimental studies, performed by authors of the present review, have demonstrated the efficacy of ELF and the new TAMMEF systems in several musculoskeletal disorders such as osteoarthritis, rheumatoid arthritis, carpal tunnel syndrome, shoulder periarthritis and cervical spondylosis. Moreover, it has been demonstrated that ELF and TAMMEF systems are not only effective, but also safe, from clinical and experimental point of view. In fact, clinical trials did not reported any undesired side effect, while in vitro studies showed that ELF EMFs did not induce uncontrolled cell proliferation, did not affect cell viability and did not induce apoptosis. With their efficacy and safety, ELF and even more the new TAMMEF systems represent a valid complementary or alternative treatment to standard pharmacological therapies in reducing both pain and inflammation of patients affected by musculoskeletal disorders.
Extended-Spectrum Beta-Lactamases Producing Klebsiella Species Isolated from Several Major Hospitals in Iran
Authors: Mohebi R. , Ghafourian S. , Sekawi Z. , Neela V. , Raftari M. , Aboualigalehdari E. , Sadeghifard N.
Published: January 2017

Klebsiella pneumoniae is a common hospital-acquired pathogen, causing urinary tract infections, nosocomial pneumoniae, and intra-abdominal infections. K.pneumoniae is also a potential community acquired pathogen. The aims of this study are to determine epidemiology of ESBL-producing K.pneumoniae and Koxytoca in Iran during different seasons, to determine the prevalence of blaTEM, SHV and CTX-M responsible for ESBL production among ESBL-producing K.pneumoniae and Koxytoca in the different wards and hospitals in Iraa Klebsiella spp producing ESBLs were identified by phenotypic and genotyping methods. The findings in this study revealed that 36.5%, 51.7% and 45.6% of K.pneumoniae were producing ESBLs in Ham, Milad and Emam Reza hospitals, respectively. The results revealed blaSHV was responsible for ESBLs production. The analysis showed significant difference of ESBLs production by K.pneumoniae in winter (53%) in comparison to the other seasons P≤ 0.01. The results also showed a significant difference in blaSHV that was the dominant gene responsible for ESBL production P≤ 0.049 but no significant difference was observed between blaTEM and blaSHV. The results showed that the highest ESBL production was found in Koxytoca isolated from patients in Emam Reza Hospital and the lowest frequency of ESBL production was found among Koxytoca in Ham hospital. BlaSHV was found as dominant gene responsible for ESBLs production by K.pneumoniae and Koxytoca, followed by blaCTX-M.
Plasma and Adipose Tissue Levels of Selected Growth/Inhibitory Factors, Proteolytic Enzymes and Sphingosine-1-Phosphate in Humans
Authors: Błogowski W. , Serwin K. , Sałata D. , Budkowska M. , Dołęgowska B. , Łokaj M. , Prowans P. , Starzyńska T.
Published: January 2017

Recent studies have shown that adipose tissue (AT), while implicated in orchestrating the sophisticated process termed “immunometabolism,” may also serve as a potential niche for various bone marrow-derived (stem) cells. However, at present, the direct biochemical and immunomodulatory composition of the human AT environment has not been studied. Several substances that might play a crucial role in regulating stem cell migration and/or homing to AT, have been implicated, namely, hepatocyte/vascular endothelial growth factor (VEGF/HGF), leukemia inhibitory factor (LIF), and sphingosine-1-phosphate (SIP). Therefore, we examined and compared the AT concentrations of these substances between plasma, subcutaneous, and omental AT samples derived from 35 generally healthy subjects. VEGF, HGF, LIF, and metalloproteinases (MMP)-2 and MMP9 levels were measured using ELISA, and S1P concentrations were analyzed using reverse-phase high performance liquid chromatography. We found that AT levels of analyzed growth/inhibitory factors were generally comparable (VEGF and LIF) or even higher (HGF) than the corresponding levels in the peripheral blood, particularly in overweight/obese subjects. In subcutaneous AT, significantly lower VEGF and LIF concentrations were observed, and these were accompanied by higher MMP levels. No depot-specific differences in S1P concentrations were found in all examined groups. Moreover, we established several associations between analyzed molecular substances and body composition, BMI, or adiposity index of the examined patients. In conclusion, our study revealed that human AT possesses relatively high levels of selected growth/inhibitory factors and of chemoattractants involved in the regulation of stem cell trafficking, and these factors are associated with the metabolic status of an individual. Further studies are needed to clearly establish the role of these factors in the regulation of bone marrow-derived (stem) cell homeostasis and homing in human AT.
Indole-3-Carbinol Exerts Sex-Specific Effects in Murine Colitis
Authors: Benson J.M. , Beamer C.A. , Seaver B.P. , Shepherd D.M.
Published: January 2017

Due to the severe adverse effects that can accompany conventional therapies for Crohn's disease, the search for natural complementary therapies has increased dramatically in recent years. Indole-3-carbinol (I3C), a constituent of cruciferous vegetables, possesses anti-inflammatory properties; however, its effects on intestinal inflammation have yet to be evaluated. To test the hypothesis that I3C dampens intestinal inflammation, C57B1/6 mice were treated with I3C and exposed to 2,4,6-trinitrobenzenesulfonic acid (TNBS) to induce colitis. Several parameters of disease severity and inflammation were subsequently evaluated. I3C dampened the disease severity, as indicated by decreased body weight loss and decreased severity of clinical signs. Interestingly, this effect was observed in female but not male mice, which displayed a trend towards exacerbated colitis. Differential effects were observed in the profiles of cytokine production, as the production of pro-inflammatory cytokines was increased in males. The sex-specific effect of I3C in TNBS-induced colitis is a novel finding and warrants further investigation since this is a common dietary compound and is also available commercially.
Overexpression of Recombinant Lipase from Burkholderia Cepacia in Escherichia Coli
Authors: Raftari M. , Ghafourian S. , Sadeghifard N. , Bakar F. Abu , Saari N. , Sekawi Z.
Published: January 2017

This study attempts to clone and express the extracellular lipase from Burkholderia cepacia in Escherichia coli using pET system as well as to determine the enzyme activity of recombinant lipase. The extracted DNA from B. cepacia was used as a template for amplifying lipase gene, and then the lipase gene was subcloned into pET-32a and subsequently transformed into E. coli BL21. Media assay and SDS-PAGE were carried out to analyse the results. Nucleotide sequencing of the DNA insert from the clone revealed that the lipase activity corresponded to an open reading frame consisting of 1092 bp coding for a 37.5-kDa protein. The successful expression of lipase was confirmed by obtaining blue color colonies on Nile Blue Sulphate Agar and big band at 37.5-kD size on SDS-PAGE. The enzyme activity assay also showed the high lipase activity around 590 μg lipase ml−1 culture 30 min−1 of recombinant E. coli BL21. The specific lipolytic activity of the recombinant lipase was 185 U/mL which is around 35-fold higher than the native baseline. The findings suggest that the crude recombinant lipase has potential application in digestion of lipids and fatty acids. In conclusion, the results of the current study showed a lipase gene encoding an enzyme with non-specific hydrolysis activity, which could be applied as lipase biosensor for digestion of lipids in food and medicine as well as oil-contamination treatment.
Long-Term Adjustment of Stable Asthma Treatment with Fractional Exhaled Nitric Oxide and Sputum Eosinophils
Authors: Malerba M. , Ragnoli B. , Radaeli A. , Ricciardolo F.L.M.
Published: January 2017

Current approaches to control asthma do not involve direct assessment of airway inflammation. The aim of this study is to assess whether the therapeutic adjustments of steroid treatment according to a stepwise algorithm based on sputum Eosinophils (sEos) and fractioned exhaled Nitric Oxide (FeNO) were effective in maintaining the stability of a group of stable asthmatic patients during a twelvemonth follow-up. Fourteen asthmatic patients, treated for asthma according to a previously published protocol, were enrolled in the study. The patients underwent clinical evaluation, pulmonary function tests, measuring of airway hyperresponsiveness to methacholine, and determination of FeNO and sEos at visit 1. These procedures were repeated after 6 and 12 months (Visits 2 and 3, respectively). Symptoms score gradually improved during the study (p=0.008), no changes were observed in the frequency of clinical asthma exacerbations or in airway hyperresponsiveness to methacholine. At the end of the study both sEos and FeNO were significantly improved (p=0.011 and p=0.003, respectively) and at visit 3 the median steroid dose was reduced (p=0.039) in accordance with the improving of symptoms score, FeNO and sEos values. A direct relationship was observed between the difference of FeNO values and the difference of sEos registered between visits 1 and 2 (r2=609, p0.001) and between visits 2 and 3 (r2=646, p<0.001). In conclusion, long-term titration of asthma inhaled steroid treatment based on sEos and FeNO values was able to provide long-term clinical stability and improvement to the asthmatic patients studied, without significant increases in the steroid dose.
Analysis of Sperm Motility Related to Transcriptional Alterations of Mitocondrial Genes in Males Affected by Infertility
Authors: Pietropaolo V. , Passariello C. , Bellizzi A. , Virga A. , Anzivino E. , Rodio D.M. , Fioriti D. , Bertozzi M.A. , Voliani S. , Scaravelli G. , Antonini G. , Gentile V.
Published: January 2017

Infertility is a problem afflicting about 1/6 couples, and in 40% of cases this is primarily due to the male. Male infertility is a multifactorial pathology and it seems mainly related to sperm motility or sperm number. However, a diagnosis of infertility is frequently not followed by a precise explanation of its cause, reflecting our poor understanding of the spermatogenesis-related regulatory mechanisms and gene expression profiles. Therefore, this study was design to investigate the relative gene expression of a specific gene profile in ejaculate spermatozoa of men affected by infertility. This profile included 13 mitochondrial gene encoding subunits of respiratory chain and 7 nuclear sperm motility-related genes. We used values of progressive sperm motility (PR) to separate subjects affected by infertility into two groups, showing PR values higher (H group) or lower (L group) than the mean of the sample, and to classify fertile men (control group). We did not obtain a statistically significant difference in nuclear gene expression patterns in spermatozoa among these three groups. On the other hand, we observed an over-expression in 11/13 tested mitochondrial genes in the population of infertile males with altered sperm motility compared to the control group. This over-expression led us to speculate that there is an abnormal mRNA transcription of these 11 subunits, that impaired the normal energy supply ensuring sperm motility. Regarding the under-expression of 2/13 tested mitochondrial genes, we could assume that the spermatozoa mtDNA has accumulated mutations involving these two genes (CYB and ND4L). In conclusion, our results will provide useful information for the development of molecular diagnostic tools for clinical assessment of sperm health. However, further investigation into other sperm-related genes is needed to establish their roles in male fertility.
Clinical, Microbiological and Inflammatory Evidence of the Efficacy of Combination Therapy Including Serratiopeptidase in the Treatment of Periimplantitis
Authors: Passariello C. , Lucchese A. , Pera F. , Gigola P.
Published: January 2017

The present study was aimed to evaluate the effect of introducing administration of the proteolytic enzyme serratiopeptidase in the combined mechanical-antibiotic treatment of periimplantitis (PI). Two randomized groups of 64 adults with a diagnosis of PI were studied over a 6-month period. All patients were treated with a combined mechanical and antibiotic protocol for 15 days. The experimental group (EG) was administered antibiotic and serratiopeptidase, while the control group was administered antibiotic alone. To evaluate the effects of the two treatment protocols, clinical and radiographic indices, the concentration of IL-lβ, IL-6 and TNF-α in the gingival crevicular fluid, the amount of total bacteria] DNA and the presence of specific bacteria were assessed at baseline and at 6 months from treatment. Success rates of combined treatments at 6 months were 96.9% and 78.1% for the EG and CG respectively ( P≤0.01). Implants of the EG showed greater enhancement of clinical, microbiological and inflammatory parameters as compared to those of the CG. Microbiological analyses showed that resistance to combined therapy was constantly associated with the isolation of bacterial species that are not common periodontal pathogens (mainly S.aureus and P.aeruginosa). The data demonstrate that the addition of serratiopeptidase to combined mechanical-antibiotic treatment protocols of periimplantitis significantly improves outcomes and suggest that serratiopeptidase acts at different levels during the healing process.
Impact of IL-33 on PGD2 Generation by Activated Human Cord Blood-Derived Mast Cell: Lack of Effect on Tryptase Release
Authors: Nicoletti M. , Maccauro G. , Tripodi D. , Saggini A. , Potalivo G. , Castellani M.L. , Conti F. , Rosati M. , Tomato E. , Caraffa A. , Antinolfi P. , Conti P. , Theoharides T.C.
Published: January 2017

Mast cells are important not only in allergic reactions, but also in inflammation and are involved in a variety of responses including the immediate release of potent inflammatory mediators after activation by cross-linking of FcεRI molecules. Prostaglandin D2 (PGD2) is a major cyclooxygenase metabolite of arachidonic acid produced by mast cells and it is released following allergen challenge in allergic diseases. IL-33 is an iflammatory cytokine which is critically involved in the regulation of in vitro and in vivo cyclooxygenase production, providing a potential therapeutic target for inflammatory disorders. In this study, using human derived umbelical cord blood mast cells, we show that IL-33 (50 ng/ml), and calcium ionophore A 23187 (0.5 μg/ml), compound 48/80 (10−5 M) or anti-IgE (10 μg/ml), enhaced the production of PGD2 and this effect was inhibited by indomethacin. However, IL-33 was unable to induce tryptase release in these cells. These effects confirm the inflammatory property of IL-33 by stimulating PGD2 but not tryptase in human mast cells. The inhibitory effect of this new cytokine may have a potential therapeutic response in allergic and inflammatory diseases.
Pseudoxanthoma Elasticum and Light-Chain Amyloidosis
Authors: Carlesimo M. , Abruzzese C. , Cortesi G. , Ciccone A.M. , Poggi C. , Lombardi M. , Moscetti A. , La Verde G. , Mari E.
Published: January 2017

Pseudoxanthoma elasticum is a heritable disorder of connective tissue characterized by cutaneous, vascular and ocular changes that result from the accumulation of fragmented elastic fibres. Even though the etiopathogenesis is not still completely understood, in recent years in literature some Authors have considered pseudoxanthoma elasticum as a metabolic disorder. We present the case of a 45-year-old man affected by pseudoxanthoma elasticum and light-chain amyloidosis and we discuss the possible reasons that led to this association.
Antiproliferative Properties of the Serotonin Receptor Antagonist Ondansetron Correlate with Increased Nitric Oxides Release and Inducible Nitric Oxide Synthase Activity in the Acute Lymphoblastic Leukemia Cell Line REH
Authors: Prada J. , Shalapour S. , Pfau M. , Henze G. , Seeger K.
Published: January 2017

A recent report from our group described that the (serotonin receptor-3)-antagonist ondansetron exhibits antiproliferative effects in the B-cell precursor acute lymphoblastic leukemia (BCP-ALL) cell line REH. Furthermore, after each application of ondansetron to cultured REH cells, significant increases (+23%) in the concentration of nitric oxides (NO) were observed in the cell supernatants after 72 hours incubation in standard conditions, and this effect was found to correlate with the described antiproliferative activity. This feature was further confirmed by using mRNA dot blot hybridizations with a specific gene probe for the inducible NO-synthase (iNOS), yielding significant increases (+100%) of iNOS mRNA, which were found to widely correlate with the detected increases of NO release, and also with the previously described antiproliferative effects. The presented results are the first report on high specific pro-inflammatory features of a (serotonin receptor 3)-antagonist in a BCP-ALL cell line, which are associated with previously described antiproliferative properties.
Implants Inserted in Mandible: A Case Series
Authors: Fanali S. , Lopez M. A. , Bassi M. Andreasi , Confalone L. , Carnevali G. , Carinci F.
Published: January 2017

It is generally accepted that the mandible (especially in the interforaminal region) has better bone quality than the maxilla, and this fact is probably the reason why several reports are available regarding implants inserted into the mandible. Since no report is available on a new type of implants, a retrospective study was performed. A total of 185 two-piece implants (FMD srl, Rome, Italy) were inserted in mandible, 102 in female and 83 in males. The median age was 58 ± 13 (min-max 25–80 years). Implants replaced 14 incisors, 7 cuspids, 49 premolars and 115 molars. Implant' length was x ≤ 10 mm, 10,30 ≤ x ≤ 12.30, equal to 13 mm and x ≥14 mm in 80, 90,13 and 2 cases, respectively. Implant' diameter was narrower than 3.5 mm, equal to 3.8 mm and wider than 4.0 mm in 25, 17,143 cases, respectively. There were 36,41,106 and 2 Elisir, I-fix, Shiner, and Storm implant types, respectively. One implant was lost, survival rate = 98.15%. Among the studies variables immediate loaded implants on single tooth rehabilitations (p=0.017) have a worse clinical outcome. Then peri-implant bone resorption (i.e. delta IAJ) was used to investigate SCR. Among the remaining 184 implants, 20 fixtures have a crestal bone resorption greater than 1.5 mm (SCR = 89.13). Statistical analysis demonstrated signifiance only for surgeon (p=0.001). In conclusion FMD implants are reliable devices for oral rehabilitation with a very high SCR and SVR.
Effect of Narrow Diameter Implants on Clinical Outcome
Authors: Fanali S. , Lopez M. A. , Bassi M. Andreasi , Confalone L. , Zollino I. , Carinci F.
Published: January 2017

Narrow diameter implants (NDI) (i.e. diameter 3.75 mm) are a potential solution for specific clinical situations such as reduced interradicuiar bone, thin alveolar crest and replacement of teeth with small cervical diameter. NDI have been available in clinical practice since the nineties but only a few studies have analyzed their clinical outcome. Since no report is available on a new type of implants, a retrospective study was performed. A total of 47 narrow diameter (i.e. x ≤ 3.40 mm) two-piece implants (FMD srl, Rome, Italy) were inserted, 35 in females and 12 in males. The median age was 60 ± 11 (min-max 30–80 years). Implants were inserted 22 in the maxilla and 25 in the mandible; they replaced 11 incisors, 3 cuspids, 21 premolars and 12 molars. Implant' length was shorter than 10 mm, 10.30 ≤ x ≤ 12.30, equal to 13 mm and longer than 13 mm in 17,28,1 and 1 fixtures, respectively. Implant' diameter was narrower than 3.5 mm. There were 3,18 and 26 Elisir, I-fix and Shiner implant types. No implant on single tooth rehabilitations was lost and thus survival rate was 100%. Then peri-implant bone resorption (i.e. delta IAJ) was used to investigate SCR. Seven fixtures have a crestal bone resorption greater than 1.5 mm (SCR = 85.1). Statistical analysis demonstrated that diabetes (p=0.044) and smoke (p=0.001) have a higher peri-implant crestal bone resorption. In conclusion FMD implants are reliable devices for oral rehabilitation with a very high SCR and SVR although smoker and diabetic patients have a worse clinical outcome.
Management Missing Later Incisor in Monozygotic Twins: Two Case Reports
Authors: Mingardi M. , Fumagalli T. , Cambini A. , Giacomello M.S. , Caccianiga G.
Published: January 2017

Tooth loss of anterior region is the result of congenital anomaly or of a traumatic event. Maxillary lateral incisor is very important in masticatory function because permit lateral protrusion movement of mandible and is a key point in aesthetic of smile. Tooth agenesis is one of the most common developmental anomalies in man and it often is a feature of syndromes. Tooth developmental results by interactions of genetic and environmental factors, in particular mutations in MSX1, PAX9 contribute tooth agenesis, but also radiotherapy, chemotherapy, maternal systemic diseases aid genetic aberrations. Upper lateral incisor is in a unfavorable anatomical position because it's in fusion area of facial processes for this it's the most common tooth loss in oral cleft. The management of these patients is complex and it includes an orthodontic, prosthetic and surgical analysis This report addresses the fundamental considerations related to replacement of congenitally missing lateral incisors by a team approach.
The Stomatognathic System's Role in the Postural Stabilization in Subjects Suffering from Whiplash Injury. Part 2: Patients with Malocclusion
Authors: Giacomello M.S. , Bertella L. , Crestale C. , Perego S. , Caccianiga G.
Published: January 2017

Aim of this study is analyze the effects of dental occlusion on postural stability in patients with previous whiplash and malocclusion. Materials and methods: 30 adult patients with previous whiplash and malocclusion (crossbite, mandibular shift, bad alignment, edentulous spaces) were selected. The patients were suffering from masticatory muscles pain caused by clenching during sleep. Exclusion criteria: use of psych drugs. To simulate the conditions of sleep (with a reduction of proprio- and esteroceptive inputs for postural management performed by CNS), we used postural Romberg analysis (feet together, closed eyes) on stabilometric platform changing occlusal parameters only, under the following conditions: 1) in occlusal rest position, 2) in centric occlusion, 3) in clenching, 4) with occlusal disengage with cotton rolls and 5) in clenching on cotton rolls. Time of each test: 15 seconds. It was performed a statistical analysis with T-test comparing between them the mean of all tests. Results: stabilometry data (postural ball and ellipse) show better postural with occlusal disengage (in centric and in clenching), and a worsening in all tests with centric occlusion (with statistically significant difference - p<0.05). Conclusions: postural instability caused by whiplash increases muscles tone and dental clenching as compensatory effects, with onset of facial pain and a worsening of TMJ health in subjects with malocclusion. An occlusal disengage (like an occlusal splint to reduce clenching) induces better postural performances. Sostomatognathic system becomes a source of postural dysfunction and a cause of postural worsening.
The Use of Laser Diodes Superpulses in Implantology
Authors: Caccianiga G. , Cambini A. , Rey G. , Paiusco A. , Fumagalli T. , Giacomello M.S.
Published: January 2017

Diode laser is a laser, usually between 810 and 980 nm wavelength, able to work on hard and soft tissues, as periodontal tissues and bone. The use of Diode laser is recommended during oral surgery or periodontal therapy, when it is necessary to erase any periodontal bacteria evolved in periodontal disease, in combination with hydrogen peroxide (photodynamic therapy), and also, by its biostimulating effects, to improve the healing of the regenerative procedures, as sinus lift, bone crestal expansion or to assist implant surgery. This article shows two cases report in which diode lasers are evolved. Diode lasers in association with Hydrogen Peroxide on utilizing the decontaminant effects oh Photodynamic Therapy, and to prepare the area defects for a regenerative surgery.
Photodynamic Therapy (Association Diode Laser/Hydrogen Peroxide): Evaluation of Bactericidal Effects on Periodontopathy Bacteria: An in Vitro Study
Authors: Caccianiga G. , Rey G. , Fumagalli T. , Cambini A. , Denotti G. , Giacomello M.S.
Published: January 2017

The main cause for the development of periodontitis is the accumulation of subgingival microbial deposits organized tightly to the porous surface of the tooth cementuni. The growth of the microbial populations and the immunological reaction of the host organism are responsible for the destruction of periodontal ligament apparatus. The primary goal in the treatment of periodontitis is the complete removal of subgingival bacterial deposits. The large amount of evidence establishing a microbial etiology for periodontitis (has been the basis for the development of antimicrobial treatment approaches. Photodynamic therapy (PDT) employs a non-toxic dye, termed a photosensitizer (PS), and low intensity visible light which, in the presence of oxygen, combine to produce cytotoxic species. In this in vitro protocol study it was tested the bactericidal efficacy of Diode laser (Oralia), of Hydrogen Peroxyde and the association Diode Laser-Peroxyd Hydrogen (PDT) on Prevotella intermedia, Peptostreptococcus micros and Fusobacterium nucleatum, three of the most aggressive bacteria envolved on periodontal disease.
Crestal Bone Remodeling around Platform Switched, Immediately Loaded Implants Placed in Sites of Previous Failures
Authors: Quaranta A. , Cicconetti A. , Battaglia L. , Piemontese M. , Pompa G. , Vozza I.
Published: January 2017

The possibility to insert immediately-loaded implants in post-extraction sites where previously inserted implants had failed and had never received a prosthesis has not been sufficiently studied to the present day. Only few articles in the literature address this problem. The aim of the present study was to evaluate crestal bone remodeling around implants with a platform-switched design inserted and loaded immediately after the failure of the previous implants. This study has a follow-up of 36 months. Failed implants that had never received a prosthesis have been removed in 68 patients. In the present study 10 patients, whose bone crest measured at least 8 mm in width, were selected and 16 immediately loaded implants with a platform-switched design and a sandblasted and acid etched SLA surface, (Winsix®, Biosaf srl, Italy) were placed. Statistically significant differences were observed in crestal bone remodeling between 12 and 36 months (p<0.05) and between 24 and 36 months (p<0.001), although no statistically significant differences were found between 12 and 24 months (p>0.05). The values of periodontal indices have sometimes been borderline, although they returned normal later on. The cumulative success-rate of all the 16 implants inserted was of 93.75%, while the survival rate was 100%. Results show that immediately loaded platform-switched implants are a practicable solution for the rehabilitation of sites of early failed implants.
Oral Lichen Planus: Novel Acquisitions in the Pathogenesis and Treatment
Authors: Saraceno R. , Lorè B. , Ventura A. , Nisticò S.P. , Perugia C. , Chimenti S. , Bianchi L.
Published: January 2017

Lichen planus (LP) is a mucocutaneous disease of chronic inflammatory nature, commonly seen in dermatological and dental clinics; it is a relatively common disorder of stratified squamous epithelia, frequently exclusively involving the oral cavity. Oral Lichen Planus (OLP) is often asymptomatic, the atrophic-erosive form can cause symptoms ranging from burning sensation to severe pain, interfering with speaking, eating, and swallowing. Lichen planus is regarded as a premalignant lesion. This review discusses the role of hepatitis C virus (HCV), bacterial and fungal infection in LP. Analysing the seroprevalence of HCV infection in LP patients and patients with oral OLP in particular, which was the case in the vast majority of studies, the association varied from 0% to 62% and seemed to be connected to the high HCV seroprevalence in the general population. Candida albicans is present in about 37% of oral LP lesions. The aim of this review is to summarize what is new in the pathogenesis and treatment of OLP.
Impact of Mast Cells in Rejection of Allografts
Authors: Kritas S.K. , Saggini A. , Varvara G. , Murmura G. , Caraffa A. , Antinolfi P. , Tomato E. , Pantalone A. , Neri G. , Frydas S. , Rosati M. , Tei M. , Speziali A. , Saggini R. , Pandolfi F. , Cerulli G. , Theoharides T.C. , Conti P.
Published: January 2017

Mast cells in the tissue are located close to nerves in and around the small vessels where they orchestrate important immune response after antigen recognition through Toll-like receptors. Mast cells can activate T and B lymphocytes and dendritic cells and have been postulated to act directly within tissue allografts and/or to induce indirect effects via inflammatory mediator release, therefore they have been shown to play an indispensable role in allograft tolerance. Major limitation in the success of transplantation is the immune response of the recipient to the donor tissue. The failure of tissue grafting is caused by an inflammatory reaction called rejection. Mast cells play a role during immune response and are elicited with transplanted allograft and also may exhibit their immune-regulatory effects directly through secretion of modulatory cytokines and activation of metabolic pathways. However, the role of mast cells in transplantation is poorly understood. The most severe rejection episodes have been found in patients with an increased number of mast cells. Mast cell mediators which can activate latent forms of TGF-β or increase angiotensin II levels are capable of inducing fibrosis through various mechanisms, activating fibroblasts and inducing collagen synthesis. Mast cells are also implicated in regulatory T-cell functions and are required to sustain peripheral tolerance via Treg, therefore there is an interaction between mast cells and Treg cells. Treg create IL-9 in enhancing mast cell growth and Chemotaxis, suggesting that Treg and mast cells form a functional unit that mediates graft tolerance. In this study we concentrate our attention on the role of mast cells in rejection of allografts and try to understand the role of mast cell-related immune mechanisms in organ transplantation.
MG63 and MC3T3-E1 Osteoblastic Cell Lines Response to Raloxifene
Authors: Muzio L. Lo , Santarelli A. , Orsini G. , Memè L. , Mattioli-Belmonte M. , De Florio I. , Gatto R. , Gallusi G. , Nocini P.F. , Bertossi D. , Emanuelli M. , Putignano A. , Bambini F.
Published: January 2017

Bone resorption in edentulous regions often results in inadequate ridge for implant osseointegration. In order to overcome this problem, the use of osteoconductive biomaterials has been proposed as a carrier for different types of pharmacological molecules. Since raloxifene, a drug used in osteoporosis therapy, inhibits the osteoclast, but not osteoblast functions, it has been suggested to improve recovery during implant surgery. The present work evaluated in vitro the effect of raloxifene on two different cell populations: the human osteoblast-like cells (MG63) and osteoblasts derived from rat calvaria (MC3T3-E1). The morpho-functional investigations carried out showed a different behavior of the two cell lines. Raloxifene showed a stimulatory effect towards MG63 cell proliferation with a significant increase in cell viability after 7 days of culture. On the contrary, MC3T3-E1 cells showed a significant reduction in cell viability, when compared with the same cells at 72 h, or with the control cell population. The predominantly proliferative effect of raloxifene on MG63 cells is partly confirmed by the reduction of alkaline phosphatase activity, an early marker of osteoblast differentiation. The different effect of raloxifene on osteoblastic population in relationship to the type and age of the cell is an issue that needs further investigation.
Underestimation of Atypical Lobular Hyperplasia and Lobular Carcinoma in Situ at Stereotaxic 11-Gauge Vacuum-Assisted Breast Biopsy
Authors: Gatta G. , Di Grezia G. , Ancona A. , Capodieci M. , Coppolino F. , Rossi C. , Feragalli B. , Iacomino A. , Cappabianca S. , Grassi R.
Published: January 2017

The aims of this study are to determine the frequency of diagnosis of atypical lobular hyperplasia (ALH) and lobular carcinoma in situ (LCIS) at stereotaxic-guided 11-gauge vacuum-assisted breast biopsy (VABB) and to assess the rate of underestimation of these at subsequent surgical excision and follow-up. Moreover, we aimed to define clinical, radiological and histological features of nonpalpable lesions in core biopsies that predict the lesion upgrade. Retrospective review of 11-gauge VABB was performed to identify the underestimation rate of nonpalpable lesions diagnosed as ALH or LCIS at VABB. Thirteen cases of ALH and 36 cases of LCIS were sent to surgery, 29 cases of ALH and 14 cases of LCIS were sent to follow-up. The clinical, mammographic and histologic features were assessed. The correlation between mammographic BI-RADS score and histological B-classification for both ALH and LCIS lesions were performed by Pearson's test. Of 1,765 patients enrolled, lobular lesions (ALH and/or LCIS) occurred in 82 cases, and underestimation arose in 9 (10.9%). Two cases of underestimated ALH were upgraded to invasive lobular carcinoma and one to invasive ductal carcinoma. One case of underestimated LCIS was upgraded to ductal carcinoma in situ, two to invasive ductal carcinoma and three to invasive lobular carcinoma. The histology of the core and surgical specimens were compared. A significant difference was seen in the BI-RADS score (4–5 in 91% of underestimated lesions), and the size of the lesions (≥ 1.5 cm) for underestimated cases versus accurately diagnosed cases (p<0.001). Further significant parameters predictive for malignancy were the incomplete lesion removal by VABB and the presence of associated different breast lesions in the specimen. In conclusion, as far as ALH is concerned, we propose surgery as first choice when at least one of the following condition is respected: positive history for breast carcinoma, lesion >1.5cm, co-presence of high-risk lesions in the sample, signs of ductal involvement, high histological grading for atypia and follow-up in the other cases. Surgery is recommended in all cases of LCIS:
Correlation between the Expression Level of the MDM Homolog Gene and Inflammatory Lesions in Different Populations of the Model Organism Mytilus Galloprovincialis from Polluted Areas of the Gulf of Naples (Southern Italy)
Authors: Carella F. , Aceto S. , Fiengo M. , Salemme M. , Aniello F. , Muzio L. Lo , De Vico G.
Published: January 2017

In vertebrate species, the MDM2 and MDM4 gene paralogs negatively regulate the activity of p53 family members and are involved in the development of a number of tumor types. Comparative genomic analyses have shown the presence of a single mdm homolog gene in invertebrates; its interaction with p53 and its involvement in tumor pathogenesis was demonstrated only in the mussel Mytilus trossulus. In addition to p53-related activity, a pro-inflammatory role for MDM2 in mammals has recently been described. In the present paper, we report the Real-time RT-PCR expression analysis of the mdm homolog gene in digestive gland tissue of Mytilus galloprovincialis collected from four different sites in the Campania region (Italy) during a single year. Our results revealed a positive correlation between the expression level of the mdm homolog and the percentage of chronic inflammatory lesions, both of which are increased during the summer period, suggesting a previously unidentified involvement of mdm in inflammatory processes in invertebrate species. Results obtained pointed out the potential interest of the use of mdm gene expression in marine food and seawater quality evaluation monitoring programs.
The Effect of Intra-Articular Hyaluronic Acid (Sinovial® One) on Knee Osteoarthritis: A Preliminary Study
Authors: Polacco A. , Zobel B. Beomonte , Polacco M. , Scarlata S. , Gasparro F. , Del Vescovo R. , Scarciolla L.
Published: January 2017

Intra-articular injections of hyaluronic acid are a valid treatment option for patients with osteoarthritis. Differences in purity, origin, and molecular weight may influence the efficacy and safety of hyaluronic acid products, therefore, we evaluated the safety, efficacy, and duration of improvements following a single intra-articular injection of a low-medium molecular weight hyaluronic acid product of bacterial synthesis, Sinovial® One, on patients with osteoarthritis of the knee. The double-blind study enrolled 21 patients (24 knees) with symptomatic knee osteoarthritis, classified into moderate, severe and very severe osteoarthritis using the Western Ontario and McMaster Universities Osteoarthritis (WOMAC) pain functional Index and the Kellgren and Lawrence scales. At four months there was improvement in measured clinical parameters in 77.6% of the 24 treated knees, particularly in patients with moderate and severe osteoarthritis (improvement in 100% and 66.7%, respectively). No local or systemic adverse events were observed. These preliminary findings suggest that Sinovial® One is safe and effective for patients with knee osteoarthritis, providing long-lasting improvement in clinical parameters.
Orthopedic Therapy and Allergic Contact Dermatitis: An Unusual Case of Double Sensitization
Authors: Tammaro A. , Abruzzese C. , Narcisi A. , Cortesi G. , Parisella F.R. , Grippaudo F.R. , Persechino S.
Published: January 2017

We report the first case of a double sensitization for two apparently non-related allergens: disperse red textile dye and topical ketoprofen. A possible explanation was that the topical gel medication could act as a vehicle between the skin and the textile red dye, underlining the importance of a “carrier-substance” to facilitate the allergen penetration and a possible sensitization to it.
Measurement of Interrupter Respiratory Resistance and Spirometry in Preschool Children: Influence of Respiratory Symptoms
Authors: Tancredi G. , de Castro G. , Rugiano A. , Zicari A.M. , Indinnimeo L. , Nicolai A. , Duse M.
Published: January 2017

Pulmonary function tests play an important role in the diagnosis and management of respiratory diseases in children. The purpose of the study was to evaluate lung function using the interrupter resistance technique (Rint) and spirometry (flow-volume and volume-time) in preschool children and to correlate the findings with respiratory symptoms. We studied 103 children (65 males, 38 females; mean age 5.2±0.7 years; range 3.6–5.8). For each child we collected family history concerning: respiratory diseases, skin prick tests, smoking during maternal pregnancy, history of gestational and neonatal period. All children performed lung function tests (Rint and spirometry) and skin prick test for inhalant and food allergens. Twenty-eight subjects (27.2%) had respiratory symptoms (RS). Expiratory Rint were performed in all subjects and spirometry was carried out on 76 children (73.8%). Spirometric indices were not statistically different between subjects without respiratory symptoms (controls) and RS children except for FEF25–75 expressed as a percentage of the predicted value (RS: 81.5±13.7% vs controls: 94.5±15.8%; p <0.001). Rint mean values were significantly higher in RS children than in controls (RS: 135.6 ±24.8% vs controls: 102.4 ±21.7%; p< 0.0001). We found a statistically negative correlation between Rint and the following Spirometric indices: FEV0.5 (R= −0.696; p < 0.0001), FEV, (R= − 0.728; p < 0.0001) and FEF25–75 (R= −0.681; p < 0.0001). In preschool children with respiratory disease we found significantly higher mean values of Rint and lower FEF25-75 than in the control group and a significant negative relationship between Rint and Spirometric indices.
A Case of Pericarditis Due to Roseomonas Gilardii in a Patient with Angiosarcoma of the Pericardium
Authors: Santino I. , Borghesi R. , Alari A.
Published: January 2017

Roseomonas gilardii is a Gram-negative slow growing coccobacillus that has been recognized as an opportunistic pathogen which can lead to infections, especially in immunocompromised and chronically ill patients. The organism is generally susceptible to carbapenems and aminoglycosides, but resistant to most of the cephalosporins. We report what we believe is the first case of a 72-year-old oncologic woman who developed a pericarditis caused by R. gilardii in Italy.
Is Minimally Invasive Spine Surgery Also Minimally Pro-Inflammatory? Muscular Markers, Inflammatory Parameters and Cytokines to Quantify the Operative Invasiveness Assessment in Spine Fusion
Authors: Lombardi G. , Grasso D. , Berjano P. , Banfi G. , Lamartina C.
Published: January 2017

Over the last decades, minimally invasive surgery (MIS) techniques entered in the surgical routine due to their major advantage in reducing the unnecessary exposure of tissue and, thus, the trauma. Even in the context of orthopedics and spine surgery these practices have been widely developed and applied. Besides the clinical outcome of the patients, few studies have quantitatively assessed the traumatic and inflammatory effects of a specific surgical technique. Indeed, currently, a universally accepted biological outcome measure, such as a panel of biochemical markers, to define the success of MIS approach is still lacking. We reviewed the literature to collect the published data regarding the quantitative analysis of trauma induced by either conventional or minimally invasive surgery with the aim of highlighting evidence useful to guide future studies. Previous publications show some evidence in support of the hypothesis that MIS approaches are less traumatic, and possibly less pro-inflammatory, than conventional ones. Creatin kinase (as a marker of muscular damage) and C-reactive protein (as a marker of systemic inflammation) seem to reproducibly follow different trends in minimally invasive surgery compared to conventional procedures. Moreover, cytokines, such as interleukin (IL)-6 and IL-10 are also promising markers in this context.
Articular and other Immune-Mediated Extra-Intestinal Manifestations in Inflammatory Bowel Diseases
Authors: Peluso R. , Iervolino S. , Vitiello M. , Bruner V. , Ambrosino P. , Manguso F. , Castiglione F. , Di Minno M.N.D.
Published: January 2017

The articular involvement in patients with inflammatory bowel diseases is included in the group of immune-mediated extra-intestinal manifestations, occurring approximately in a range from 6.2–36% of the patients. This group is also made up of the skin and eyes manifestations, that usually but not invariably are correlated with intestinal inflammatory disease activity. Rheumatic manifestations are the most frequent extra-intestinal findings of this group with a prevalence from 20–50%. They are divided into two different clinical subsets: peripheral and axial joint involvement (including sacroiliitis with or without spondylitis). Peripheral arthritis is the most frequent finding in both Crohn's disease and ulcerative colitis, occurring with a frequency ranging from 17–20%, and it is more common in Crohn's disease. Axial involvement is more common in Crohn's disease (5–22%) than in ulcerative colitis (2–6%) and generally the prevalence of sacroiliitis (asymptomatic and symptomatic) is between 12–20% and of spondylitis is between 2–16%. The IBD is also associated with other rheumatic diseases such as rheumatoid arthritis, Sjogren syndrome, Takayasu arteritis and fibromyalgia. The management of patients with EA requires an active cooperation between gastroenterologists and rheumatologists.
Genome-Wide Scan for Copy Number Variation Association with Biomarker Quantitative Trait Loci in Aging
Authors: Szigeti K. , Trummer B. , Lal D. , Doody R.S. , Yan L. , Liu S. , Ma C. ,   Texas Alzheimer Research and Care Consortium and the Alzheimer's Disease Neuroimaging Initiative
Published: January 2017

Biomarkers are emerging as important tools in the detection and monitoring of various diseases. A major limitation and challenge to effectively utilize biomarker signals is the limited understanding of factors contributing to their variance. As genetic variation is a major contributor to phenotypic variation, exploring genetic contributions is of great importance. Copy number variants (CNVs) offer an alternative genomic framework to understand contributions to phenotypic variance. A copy-number variation genome-wide association study was performed using 116 serum inflammatory biomarkers as quantitative trait in elderly normal controls to test the hypothesis that CNVs contribute to the phenotypic heterogeneity of serum biomarkers. Three chromosomal regions were associated with four biomarkers in trans. Transforming growth factor alpha (TG-alpha) serum levels were associated with CNV dosage at chr11:5,788 kb, soluble levels of receptor for advanced glycation endproducts (sRAGE) was associated with CNV dosage at chr8:40,183 kb and both thrombospondin-1 and tissue inhibitor of metalloproteinase 1 (TIMP-1) were associated with CNV dosage at chr11:18,961 kb. The CNV at chr11:5,788 kb harbors 2 olfactory genes and the introns of Tripartite motif-containing (TRIM) gene cluster TRIM5&22 while the CNV at chr11:18,961 includes the Mas-related G-protein coupled receptor member X1. These trans associations may identify novel relationships in the relevant pathways and suggest that genetic variation can contribute to biomarker levels. The detected trans-association between MRGPRX1 and thrombospondin-1/TIMP-1 could implicate a novel pathway between pain/itching and inflammation. Cataloguing all genetic variants with an effect on biomarkers will serve as a tool to interpret epidemiological studies and establish causal relationships through Mendelian randomization.