bluuebunny/crossref_metadata_2025 huggingface dataset:
Impairments to the multisensory integration brain regions during migraine chronification: correlation with the vestibular dysfunction
Authors: Dong Liang , Fan Xiaoping , Fan Yulan , Li Ximao , Li Hui , Zhou Jiying
Published: July 2023

Objectives

Migraine is often combined with vestibular dysfunction, particularly in patients with chronic migraine (CM). However, the pathogenesis of migraine chronification leading to vestibular dysfunction is not fully understood. The current study investigated whether structural or functional impairments to the brain during migraine chronification could be associated with vestibular dysfunction development.

Methods

The eligible participants underwent clinical assessment and magnetic resonance imaging (MRI) scans. Voxel-based morphometry (VBM) determined structural impairment by evaluating alterations in gray matter volume (GMV). Functional impairment was assessed by the mean amplitude of low-frequency fluctuation (mALFF). Furthermore, the resting-state functional connectivity (rsFC) of regions possessing impairment was examined with a seed-based approach. We also analyzed the correlations between altered neuroimaging features with clinical variables and performed multiple linear regression.

Results

Eighteen CM patients, 18 episodic migraine (EM) patients, and 18 healthy controls (HCs) were included in this study. A one-way ANOVA indicated the group differences in mALFF. These were located within right supramarginal gyrus (SMG), left angular gyrus (AG), middle frontal gyrus (MFG), left middle occipital gyrus (MOG), right rolandic operculum (Rol) and left superior parietal gyrus (SPG). During rsFC analysis, the CM group had more enhanced rsFC of left SPG with left MOG than the EM and HC groups. The EM group revealed enhanced rsFC of left SPG with left AG than the CM and HC groups. In multiple linear regression, after controlling for age, body mass index (BMI) and disease duration, the rsFC of left SPG with left MOG (β = 48.896, p = 0.021) was found to predict the total Dizziness Handicap Inventory (DHI) score with an explained variance of 25.1%. Moreover, the rsFC of left SPG with left MOG (β = 1.253, p = 0.003) and right SMG (β = −1.571, p = 0.049) were significant predictors of migraine frequency, accounting for a total explained variance of 73.8%.

Conclusion

The functional impairments due to migraine chronification are primarily concentrated in the multisensory integration-related brain regions. Additionally, the rsFC of SPG with MOG can predict the frequency of migraine and the degree of vestibular dysfunction. Therefore, these neuroimaging features could be potential mechanisms and therapeutic targets for developing vestibular dysfunction in migraine.
Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease
Authors: Gareau Daniel S. , RochaKim Nicholas , Choudhury Arnab , Bamkole Michael , Snuderl Matija , Zou Julia , Yaroslavsky Anna , Jacques Steven L. , Strickland Sidney , Krueger James G. , Ahn Hyung Jin
Published: July 2023

Many Alzheimer’s disease (AD) patients suffer from altered cerebral blood flow and damaged cerebral vasculature. Cerebrovascular dysfunction could play an important role in this disease. However, the mechanism underlying a vascular contribution in AD is still unclear. Cerebrovascular reactivity (CVR) is a critical mechanism that maintains cerebral blood flow and brain homeostasis. Most current methods to analyze CVR require anesthesia which is known to hamper the investigation of molecular mechanisms underlying CVR. We therefore combined spectroscopy, spectral analysis software, and an implantable device to measure cerebral blood volume fraction (CBVF) and oxygen saturation (SO2) in unanesthetized, freely-moving mice. Then, we analyzed basal CBVF and SO2, and CVR of 5-month-old C57BL/6 mice during hypercapnia as well as during basic behavior such as grooming, walking and running. Moreover, we analyzed the CVR of freely-moving AD mice and their wildtype (WT) littermates during hypercapnia and could find impaired CVR in AD mice compared to WT littermates. Our results suggest that this optomechanical approach to reproducibly getting light into the brain enabled us to successfully measure CVR in unanesthetized freely-moving mice and to find impaired CVR in a mouse model of AD.
The role of policy in supporting environmentally sustainable foodservice in healthcare: lessons from exemplar hospitals
Authors: Carino Stefanie , Collins Jorja , Malekpour Shirin , Porter Judi
Published: July 2023

Background

Foodservice in hospitals contributes to the environmental footprint of healthcare delivery. There is little known about the role of policy in supporting environmentally sustainable foodservices. The aim of the study was to explore policy in exemplar environmentally sustainable hospital foodservices from the perspective of hospital staff, toward what makes a policy effective, the limitations of policy, and the influential levels and types of policy.

Methods

A generic qualitative inquiry approach was utilized. Staff involved in foodservices were interviewed about the role of policy during 2020–2021 from 14 hospitals across nine countries. Data were analyzed using framework and thematic analysis.

Results

Policies spanned across high level policies at the level of the healthcare organization, local hospital procedures and protocols, as well as public policy from local, state/provincial and national government. Internal organizational policy was used to embed practices within the organization in the long term and help to build a shared vision and goal where public policy had lacked guidance. The creation, content and methods of communication and creating accountability made internal organizational policy successful. Public policy was most effective when it was mandatory, had clearly defined targets and funding to assist implementation. These exemplar hospitals also demonstrated attributes of policy entrepreneurs by engaging with policy makers to share their stories and lobby government for policy change.

Discussion

Policy from within the healthcare organization is an important mechanism for enabling hospitals to deliver and maintain environmentally sustainable foodservice. Public policy must be designed considering the unique implementation challenges hospitals face to ensure they are successful.
New treatment options in elderly patients with Diffuse Large B-cell Lymphoma
Authors: Arcari Annalisa , Cavallo Federica , Puccini Benedetta , Vallisa Daniele
Published: July 2023

Most patients with Diffuse Large B-cell Lymphoma (DLBCL) are old (>65 years of age) and this population is expected to increase in the following years. A simplified geriatric assessment based on a careful evaluation of the fitness status and comorbidities is essential to choose the correct intensity of treatment. Fit older patients can benefit from a standard immunochemotherapy, while unfit/frail patients frequently need reduced doses or substitution of particular agents with less toxic ones. This review focuses on new therapies (e.g., polatuzumab vedotin, tafasitamab, bispecific antibodies) that have indicated promising results in relapsed/refractory patients, particularly in cases not eligible to transplant. Some of these new drugs have been tested as single agents or in combinations as first-line treatment, aiming to improve the outcome of the traditional chemotherapy. If preliminary efficacy and safety data are confirmed in future clinical trials, a chemo-free immunotherapic approach could become an alternative option to offer a curative treatment even in frail patients.
Design and implementation of a community-based mother-to-mother peer support programme for the follow-up of low birthweight infants in rural western Kenya
Authors: Wright Jemma L. , Achieng Florence , Tindi Linda , Patil Manasi , Boga Mwanamvua , Kimani Mary , Barsosio Hellen C. , Juma Dan , Kiige Laura , Manu Alexander , Kariuki Simon , Mathai Matthews , Nabwera Helen M.
Published: July 2023

Background

Globally, low birthweight (LBW) infants (<2,500 g) have the highest risk of mortality during the first year of life. Those who survive often have adverse health outcomes. Post-discharge outcomes of LBW infants in impoverished communities in Africa are largely unknown. This paper describes the design and implementation of a mother-to-mother peer training and mentoring programme for the follow-up of LBW infants in rural Kenya.

Methods

Key informant interviews were conducted with 10 mothers of neonates (infants <28 days) from two rural communities in western Kenya. These data informed the identification of key characteristics required for mother-to-mother peer supporters (peer mothers) following up LBW infants post discharge. Forty potential peer mothers were invited to attend a 5-day training programme that focused on three main themes: supportive care using appropriate communication, identification of severe illness, and recommended care strategies for LBW infants. Sixteen peer mothers were mentored to conduct seven community follow-up visits to each mother-LBW infant pair with fifteen completing all the visits over a 6-month period. A mixed methods approach was used to evaluate the implementation of the programme. Quantitative data of peer mother socio-demographic characteristics, recruitment, and retention was collected. Two post-training focus group discussions were conducted with the peer mothers to explore their experiences of the programme. Descriptive statistics were generated from the quantitative data and the qualitative data was analysed using a thematic framework.

Results

The median age of the peer mothers was 26 years (range 21–43). From March-August 2019, each peer mother conducted a median of 28 visits (range 7–77) with fourteen (88%) completing all their assigned follow-up visits. Post training, our interviews suggest that peer mothers felt empowered to promote appropriate infant feeding practices. They gave multiple examples of improved health seeking behaviours as a result of the peer mother training programme.

Conclusion

Our peer mother training programme equipped peer mothers with the knowledge and skills for the post-discharge follow-up of LBW infants in this rural community in Kenya. Community-based interventions for LBW infants, delivered by appropriately trained peer mothers, have the potential to address the current gaps in post-discharge care for these infants.
Ginseng and health outcomes: an umbrella review
Authors: Li Zhongyu , Wang Yang , Xu Qing , Ma Jinxin , Li Xuan , Tian Yibing , Wen Yandong , Chen Ting
Published: July 2023

Background: Ginseng consumption has been associated with various health outcomes. However, there are no review articles summarizing these reports.

Methods: PubMed, Embase, the Cochrane Library of Systematic Reviews, Scopus, CNKI and Wanfang databases were searched from inception to 31 July 2022. The Assessment of Multiple Systematic Reviews-2 (AMSTAR-2) and Grading of Recommendations Assessment, Development and Evaluation (GRADE) systems were used to assess the methodological quality and quality of evidence in each meta-analysis, and the results were summarized in a narrative form.

Results: Nineteen meta-analyses that met the eligibility criteria were identified from among 1,233 papers. The overall methodological quality was relatively poor, with only five studies being low-quality, and 14 critically low-quality. When compared with control treatments (mainly placebo), ginseng was beneficial for improving fatigue and physical function, sexual function, menopausal symptoms, metabolic indicators, inflammatory markers, unstable angina and respiratory diseases. Adverse events included gastrointestinal symptoms and potential bleeding; however, no serious adverse events were reported.

Conclusion: This umbrella review suggests that ginseng intake has beneficial therapeutic effects for diverse diseases. However, the methodological quality of studies needs to be improved considerably. In addition, it is imperative to establish the clinical efficacy of ginseng through high-quality randomized controlled trials.
Inhibitory activity of flavonoids fraction from Astragalus membranaceus Fisch. ex Bunge stems and leaves on Bacillus cereus and its separation and purification
Authors: Cui Liyan , Ma Zhennan , Li Wenhui , Ma Haihui , Guo Shang , Wang Defu , Niu Yanbing
Published: July 2023

Introduction:Astragalusmembranaceus Fisch. ex Bunge is a traditional botanical drug with antibacterial, antioxidant, antiviral, and other biological activities. In the process of industrialization of A. membranaceus, most of the aboveground stems and leaves are discarded without resource utilization except for a small amount of low-value applications such as composting. This study explored the antibacterial activity of A. membranaceus stem and leaf extracts to evaluate its potential as a feed antibiotic substitute.

Materials and methods: The antibacterial activity of the flavonoid, saponin, and polysaccharide fractions in A. membranaceus stems and leaves was evaluated by the disk diffusion method. The inhibitory activity of the flavonoid fraction from A. membranaceus stems and leaves on B. cereus was explored from the aspects of the growth curve, cell wall, cell membrane, biofilm, bacterial protein, and virulence factors. On this basis, the flavonoid fraction in A. membranaceus stems and leaves were isolated and purified by column chromatography to determine the main antibacterial components.

Results: The flavonoid fraction in A. membranaceus stems and leaves had significant inhibitory activity against B. cereus, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 1.5625 and 6.25 mg/mL, respectively. A. membranaceus stem and leaf flavonoid fraction can induce death of B. cereus in many ways, such as inhibiting growth, destroying cell wall and cell membrane integrity, inhibiting biofilm formation, inhibiting bacterial protein synthesis, and downregulating virulence factor expression. In addition, it was clear that the main flavonoid with antibacterial activity in A. membranaceus stems and leaves was isoliquiritigenin. Molecular docking showed that isoliquiritigenin could form a hydrogen bonding force with FtsZ.

Conclusion:A. membranaceus stem and leaf flavonoid fractions had significant inhibitory activity against B. cereus, and the main chemical composition was isoliquiritigenin.
Gravity’s effect on biology
Authors: Narayanan S. Anand
Published: July 2023

Gravity is a fundamental interaction that permeates throughout our Universe. On Earth, gravity gives weight to physical objects, and has been a constant presence throughout terrestrial biological evolution. Thus, gravity has shaped all biological functions, some examples include the growth of plants (e.g., gravitropism), the structure and morphology of biological parts in multicellular organisms, to its effects on our physiological function when humans travel into space. Moreover, from an evolutionary perspective, gravity has been a constant force on biology, and life, to our understanding, should have no reason to not experience the effects of gravity. Interestingly, there appear to be specific biological mechanisms that activate in the absence of gravity, with the space environment the only location to study the effects of a lack of gravity on biological systems. Thus, in this perspective piece, biological adaptations from the cellular to the whole organism levels to the presence and absence of gravity will be organized and described, as well as outlining future areas of research for gravitational biological investigations to address. Up to now, we have observed and shown how gravity effects biology at different levels, with a few examples including genetic (e.g., cell cycle, metabolism, signal transduction associated pathways, etc.), biochemically (e.g., cytoskeleton, NADPH oxidase, Yes-associated protein, etc.), and functionally (e.g., astronauts experiencing musculoskeletal and cardiovascular deconditioning, immune dysfunction, etc., when traveling into space). Based from these observations, there appear to be gravity-sensitive and specific pathways across biological organisms, though knowledge gaps of the effects of gravity on biology remain, such as similarities and differences across species, reproduction, development, and evolutionary adaptations, sex-differences, etc. Thus, here an overview of the literature is provided for context of gravitational biology research to-date and consideration for future studies, as we prepare for long-term occupation of low-Earth Orbit and cis-Lunar space, and missions to the Moon and Mars, experiencing the effects of Lunar and Martian gravity on biology, respectively, through our Artemis program.
Identification of salt stress-tolerant candidate genes in the BC2F2 population at the seedling stages of G. hirsutum and G. darwinii using NGS-based bulked segregant analysis
Authors: Shehzad Muhammad , Ditta Allah , Cai Xiaoyan , Ur Rahman Shafeeq , Xu Yanchao , Wang Kunbo , Zhou Zhongli , Fang Liu
Published: July 2023

Salinity is a major threat to the yield and productivity of cotton seedlings. In the present study, we developed a BC2F2population of cotton plants fromGossypium darwinii(5–7) andGossypium hirsutum(CCRI 12–4) salt-susceptible parents to identify salt-resistant candidate genes. The Illumina HiSeq™ strategy was used with bulked segregant analysis. Salt-resistant and salt-susceptible DNA bulks were pooled by using 30 plants from a BC2F2population. Next-generation sequencing (NGS) technology was used for the sequencing of parents and both bulks. Four significant genomic regions were identified: the first genomic region was located on chromosome 18 (1.86 Mb), the second and third genomic regions were on chromosome 25 (1.06 Mb and 1.94 Mb, respectively), and the fourth was on chromosome 8 (1.41 Mb). The reads of bulk1 and bulk2 were aligned to theG. darwiniiandG. hirsutumgenomes, respectively, leading to the identification of 20,664,007 single-nucleotide polymorphisms (SNPs) and insertions/deletions (indels). After the screening, 6,573 polymorphic markers were obtained after filtration of the candidate regions. The SNP indices in resistant and susceptible bulks and Δ(SNP-index) values of resistant and susceptible bulks were measured. Based on the higher Δ(SNP-index) value, six effective polymorphic SNPs were selected in a different chromosome. Six effective SNPs were linked to five candidate genes in four genomic regions. Further validation of these five candidate genes was carried out using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), resulting in an expression profile that showed two highly upregulated genes in the salt-tolerant speciesG. darwinii, i.e.,Gohir.D05G367800andGohir.D12G239100; however, the opposite was shown inG. hirsutum, for which all genes, except one, showed partial expression. The results indicated thatGohir.D05G367800andGohir.D12G239100may be salt-tolerant genes. We are confident that this study could be helpful for the cloning, transformation, and development of salt-resistant cotton varieties.
TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions
Authors: Parra-Aguilar Thelma J. , Sarmiento-López Luis G. , Santana Olivia , Olivares Juan Elías , Pascual-Morales Edgar , Jiménez-Jiménez Saul , Quero-Hostos Andrea , Palacios-Martínez Janet , Chávez-Martínez Ana I. , Cárdenas Luis
Published: July 2023

Arbuscular mycorrhizal (AM) fungi and rhizobia form two of the most important plant-microbe associations for the assimilation of phosphorus (P) and nitrogen (N). Symbiont-derived signals are able to coordinate the infection process by triggering multiple responses in the plant root, such as calcium influxes and oscillations, increased reactive oxygen species (ROS), cytoskeletal rearrangements and altered gene expression. An examination was made of the role of tetraspanins, which are transmembrane proteins that self-organize into tetraspanin web regions, where they recruit specific proteins into platforms required for signal transduction, membrane fusion, cell trafficking, and ROS generation. In plant cells, tetraspanins are scaffolding proteins associated with root radial patterning, biotic and abiotic stress responses, cell fate determination, plasmodesmata and hormonal regulation. Some plant tetraspanins, such as Arabidopsis thaliana TETRASPANIN 8 and TETRASPANIN 9 (AtTET8 and AtTET9) are associated with exosomes during inter-kingdom communication. In this study, a homolog of AtTET8, PvTET8-1, in common bean (Phaseolus vulgaris L. var. Negro Jamapa) was examined in roots during interactions with Rhizobium tropici and Rhizophagus irregularis. The promoter of PvTET8-1 contained several cis-acting regulatory DNA elements potentially related to mutualistic interactions, and PvTET8-1 was transcriptionally activated during AM fungal and rhizobial associations. Silencing it decreased the size and number of nodules, nitrogen fixation, and mycorrhizal arbuscule formation, whereas overexpressing it increased the size and number of nodules, and mycorrhizal arbuscule formation but decreased nitrogen fixation. PvTET8-1 appears to be an important element in both of these mutualistic interactions, perhaps through its interaction with NADPH oxidase and the generation of ROS during the infection processes.
Research progress on iron absorption, transport, and molecular regulation strategy in plants
Authors: Ning Xinyi , Lin Mengfei , Huang Guohua , Mao Jipeng , Gao Zhu , Wang Xiaoling
Published: July 2023

Iron is a trace element essential for normal plant life activities and is involved in various metabolic pathways such as chlorophyll synthesis, photosynthesis, and respiration. Although iron is highly abundant in the earth’s crust, the amount that can be absorbed and utilized by plants is very low. Therefore, plants have developed a series of systems for absorption, transport, and utilization in the course of long-term evolution. This review focuses on the findings of current studies of the Fe2+ absorption mechanism I, Fe3+ chelate absorption mechanism II and plant-microbial interaction iron absorption mechanism, particularly effective measures for artificially regulating plant iron absorption and transportation to promote plant growth and development. According to the available literature, the beneficial effects of using microbial fertilizers as iron fertilizers are promising but further evidence of the interaction mechanism between microorganisms and plants is required.
Distance education project of English idioms learning from watching YouTube
Authors: Vongpumivitch Viphavee , Yu Li-Tang , Nguyen Thuy Phuong
Published: July 2023

Idioms have been recognized to be commonly adopted by academic practitioners in their speech and writing. However, despite their common occurrence in English communication, idioms pose great challenges for non-native English speakers, as they are often ignored in English classrooms. Particularly in Vietnam, a minimal number of idioms appear in English textbooks published by the Ministry of Education, and teachers appeared to under-emphasize English idioms’ usefulness in conversation. To fill the gap, the present study adopted YouTube for Vietnamese students to undertake supplementary learning of English idiom knowledge. The study recruited 34 students and randomly assigned them to a control group and an experimental group. Both groups were required to take notes during their watching. Yet, only the experimental group would take extra exercises at the end of their watching. Immediate post-tests, delayed post-tests, and a survey were conducted. The findings demonstrate that the students in the two groups retained idiom knowledge similarly well. They were also positive towards learning English idioms through YouTube. Pedagogical implications and research suggestions are provided to conclude the study.
Work-related burnout among public secondary school teachers is significantly influenced by the psychosocial work factors: a cross-sectional study from Ethiopia
Authors: Belay Azanaw Asega , Gasheya Kassahun Ayele , Engdaw Garedew Tadege , Kabito Gebisa Guyasa , Tesfaye Amensisa Hailu
Published: July 2023

Introduction

Work-related burnout (WRB) is the degree of physical and psychological fatigue and exhaustion perceived by individuals as related to their work. Even though the condition is widespread across various occupations, teachers inevitably experience high levels of burnout in their work, which can have long-term effects on their health and well-being. However, in developing countries such as Ethiopia, the lack of reliable data on psychosocial hazards, including work-related burnout, often encumbers officials from planning preventive measures. This study investigated the prevalence and contributing factors of work-related burnout among public secondary school teachers in Gondar City, northwestern Ethiopia.

Methods

An institution-based cross-sectional study was conducted from May to June 2022. A sample of 588 teachers was recruited using simple random sampling. Work-related burnout was assessed using a standardized seven-item Copenhagen Burnout Inventory (CBI). The data were collected through a self-administered questionnaire. The collected data were entered into EpiData and analyzed using SPSS. A multivariable logistic regression analysis was used to identify factors associated with work-related burnout.

Results

The overall response rate was 94.05% (N = 553). The majority of participants, 356 (64.4%), were male. The mean (±SD) age of the participants was 38.74 (±7.65) years. This study demonstrated that the prevalence of work-related burnout among school teachers in the past 12 months was 37.4% (n = 207). High job demands, job stress, job dissatisfaction, low role clarity, and student demotivation were found to be the psychosocial work factors that significantly influenced the prevalence of work-related burnout among school teachers.

Conclusion

This study highlights the high prevalence of work-related burnout among public secondary school teachers. Psychosocial work factors such as job demands, job stress, job satisfaction, role clarity, and student demotivation were significant factors influencing work-related burnout. To reduce the condition, it is recommended to take measures to cope with high job demands, improve stress management skills, promote job satisfaction strategies, clarify teachers’ responsibilities, and use effective teaching practices to motivate students. In general, addressing psychosocial work factors needs to be central to efforts to prevent teacher burnout.
Case report: Interstitial pneumonitis after initiation of lamotrigine
Authors: Watzal Victoria , Godbersen Godber Mathis , Weidenauer Ana , Willeit Matthäus , Popper Valentin , Treiber Michael , Preiss Maximilian , Ivkic Dominik , Rabl Ulrich , Fugger Gernot , Frey Richard , Kraus Christoph , Rujescu Dan , Bartova Lucie
Published: July 2023

The second-generation anticonvulsant lamotrigine is widely used in the psychiatric field as a mood stabilizer or antidepressant augmentation therapy. Although particularly older anticonvulsants are known for their potential to cause hypersensitivity syndromes, newer antiepileptic drugs do hold a certain risk as well. Presenting a case of a 32-year-old male inpatient of African ethnicity suffering from a primary severe depressive episode in the course of a recurrent major depressive disorder, we report the occurrence of a rapid-onset drug-induced pneumonitis. Herewith, the interstitial pneumonitis occurred after the initiation of 25 mg lamotrigine as an augmentation therapy. Except for the clear temporal correlation between the administration of lamotrigine and the onset of pneumonitis, we did not reveal any further potentially causal diagnostic hints. Importantly, no relevant genetic variations of metabolizing enzymes or drug interactions resulting in lamotrigine overdosage as a potential cause of toxicity were identified. Our experience with a potentially life-threatening adverse drug reaction shortly after the initiation of the largely well-tolerated lamotrigine suggests a potential side effect under the second-generation anticonvulsant although similar adverse events are deemed to be very rare.
Comparing the effects of microgravity and amyotrophic lateral sclerosis on mouse dorsal root ganglia
Authors: Yoshikawa Masaaki , Matsukawa Mutsumi , Oshima Hideki , Ishikawa Chihiro , Li Haiyan , Kudo Takashi , Shiba Dai , Shirakawa Masaki , Muratani Masafumi , Takahashi Satoru , Uemura Mamoru , Aizawa Shin , Shiga Takashi
Published: July 2023

Introduction: Microgravity (MG) exposure causes motor deficits and decreased neuronal activity, effects that resemble the ones observed in motor neuron diseases such as amyotrophic lateral sclerosis (ALS). Several recent studies have shown that exposure to MG and ALS also impacts the sensory systems. Yet, the role of sensory impairment in this degenerative process of exposure to MG and ALS remains unknown. In this study, we aimed at elucidating how the sensory system is affected by exposure to MG and ALS.

Methods: To this end, we compared gene expression in the mouse lumbar dorsal root ganglia (DRG) of MG-exposed animals with that of control animals that remained under artificial gravity conditions. We then investigated the effects of the human superoxide dismutase 1 (SOD1) G93A mutation in a mouse model of ALS (SOD1G93A mice) on gene expression in the DRG.

Results: The overlap of genes with negatively correlated expression was greater than those with positively correlated expression between the DRG of MG-exposed and SOD1G93A mice. Additionally, genes related to Imoonglia (characteristics of both immune and glial cells) and macrophage increased in response to MG exposure, while satellite glial cell genes were expressed in response to SOD1 mutation. Next, we examined genes related to sensory neuron subtypes in the DRG. We found altered gene expression in genes related to proprioceptive and mechanoreceptive neurons in the DRG of MG-exposed and SOD1G93A mice. Remarkably, the expression of Atf3 and genes related to nociceptive neurons in the DRG of SOD1G93A mice at postnatal day (P) 120 was considerably altered, whereas MG-exposed and SOD1G93A mice at P30 presented little changes.

Discussion: These results indicate that exposure to MG and ALS affect gene expression in genes related to neurons and non-neuronal cells in the DRG, with significant differences between the effects of MG and the SOD1 mutation. Elucidation of the impact of exposure to MG and ALS pathogenesis in the DRG, including identification of the molecular pathways that regulate DRG dysfunction, will help better understand the differences in vulnerability and the triggering processes of impaired motor function associated with MG and ALS.
Mechanisms of pediatric ischemic strokes in COVID-19: a systematic review
Authors: Layug Elbert John V. , Apor Almira Doreen Abigail O. , Kuhn Rudolf V. , Tan Marilyn A.
Published: July 2023

Background

Coronavirus disease 2019 (COVID-19) has been shown to cause vasculopathic and hemostatic derangements predisposing to cerebrovascular and thrombotic disorders in adults. Data in children, however, are limited to case reports and series. Given the unique risk factors and potential pathomechanisms in children, it is imperative to characterize stroke in children with COVID-19. Understanding these mechanisms is essential in drafting an appropriate management protocol to improve outcomes in a population where stroke carries higher disability-adjusted life years.

Methods

A systematic literature search was done in MEDLINE, EMBASE, Web of Science and Google Scholar using the terms “pediatric ischemic stroke,” “cerebral sinovenous thrombosis,” “SARS-CoV-2,” and “COVID-19.” Patient demographics, clinical profile, stroke risk factors, neuroimaging findings, interventions and outcomes were recorded.

Results

The search produced 776 records. After preliminary review of titles, abstracts and selected full texts, 52 articles comprising of 74 patients were studied. The cohort has slight female predominance (51.5%), with mean age of 9.2 years (±2SD 5.6). Pediatric ischemic strokes were categorized as arterial ischemic strokes (82.40%), cerebral sinovenous thrombosis (12.20%) and combined arterial and venous strokes (5.41%). Mechanisms of ischemic stroke included thrombophilia (47.3%), vasculopathies (27%) and cardioembolism (6.8%). Twenty cases (27%) had comorbidities predisposing to stroke and only 18.9% met the criteria for multisystem inflammatory syndrome in children (MIS-C). Outcomes ranged from complete recoveries (13/58), residual deficits (35/58), and mortalities (10/58).

Conclusion

This study presents a comprehensive summary of the currently available published literature on pediatric ischemic strokes in the background of COVID-19. The clinical profiles and outcomes of patients reviewed support prior hypotheses that the virus can cause both a vasculopathy and induce a derangement in the coagulation system, predisposing to ischemic strokes.

Study registration

This paper's protocol has been registered in PROSPERO with ID number CRD42022315219.
Minimally invasive surgery as a new clinical standard for bone anchored hearing implants—real-world data from 10 years of follow-up and 228 surgeries
Authors: Cruz Leonardo Di Santana , Danieli Fabiana , Håkansson Maria Åberg , Johansson Martin Lars , dos Santos Francine Raquel , Mirândola Barbosa Reis Ana Claudia , Hyppolito Miguel Angelo
Published: July 2023

Purpose

To explore the clinical practice development of different surgical techniques when installing bone-anchored hearing implants and their associated trends in outcomes.

Design

Retrospective study of 228 bone-anchored hearing implants in 200 patients, performed over a 10-year period between 2012 and 2022 in a referral hospital.

Method

Real-world data of demography, etiology, surgical setup, complications, and audiological outcomes were collected. Eligibility criteria from clinical practice were applied.

Results

The minimally invasive technique is associated with shorter surgery duration, 20 vs. 44 min as compared to a linear incision technique. The minimally invasive technique was also associated with a lower occurrence of complications when compared to linear incision techniques (intraoperative; 1.8% vs. 4.9%, postoperative; 49% vs. 66%). Most differences were seen in complications relating to skin and wound healing.

Conclusion

Adoption of a minimally invasive surgical technique for the installations of bone-anchored hearing implants can reduce surgical complexity without compromising safety aspects or clinical benefits.
Participatory assessment of management and biosecurity practices of smallholder pig farms in North East India
Authors: Singh Mahak , Pongenere Nungshitula , Mollier R. T. , Patton R. N. , Yadav Rekha , Katiyar Rahul , Jaiswal Priyanka , Bhattacharjee M. , Kalita H. , Mishra V. K.
Published: July 2023

The present study was aimed at describing the pig production system, farm management, pig movement, and existing biosecurity level of smallholders' pig production system in North East India. A cross-sectional survey of 1,000 pig producers in four districts (two urban and two rural) in core pig-producing regions of India, where ASF occurrence had been reported, was conducted. The mean pig population was significantly (p < 0.05) higher in urban districts. In urban districts, most of the pig houses were isolated but located on the roadside, while in rural districts, commune pig houses along the roadside were more common. The majority of the respondent purchased (91%) or sold (60%) the pigs during the past 12 months. Swill feeding was common in the entire study area. The majority of the respondent (80%) in rural districts were unaware of ASF. Significant pig trade of live pigs and pork products was observed in the urban district. In the case of on-farm biosecurity measures, only 6.9% of respondents had fencing for the pig farm, 99.3% did not have provision for a footbath, and only 17.2% of the respondents restricted visitors' access to the pig farm. The study revealed that the pig production system is dominated by smallholding units with a frequent introduction or exit of pigs along with poor on-farm biosecurity measures. With the current level of farm management and biosecurity practices, smallholder pig farmers are at an increased risk of ASF and other contagious diseases.
Vessel Velocity Estimation and Docking Analysis: A Computer Vision Approach
Authors: de Andrade João V. R. , Fernandes Bruno J. T. , Izídio André R. L. C. , da Silva Filho Nilson M. , Cruz Francisco
Published: July 2023

The opportunities for leveraging technology to enhance the efficiency of vessel port activities are vast. Applying video analytics to model and optimize certain processes offers a remarkable way to improve overall operations. Within the realm of vessel port activities, two crucial processes are vessel approximation and the docking process. This work specifically focuses on developing a vessel velocity estimation model and a docking mooring analytical system using a computer vision approach. The study introduces algorithms for speed estimation and mooring bitt detection, leveraging techniques such as the Structural Similarity Index (SSIM) for precise image comparison. The obtained results highlight the effectiveness of the proposed algorithms, demonstrating satisfactory speed estimation capabilities and successful identification of tied cables on the mooring bitts. These advancements pave the way for enhanced safety and efficiency in vessel docking procedures. However, further research and improvements are necessary to address challenges related to occlusions and illumination variations and explore additional techniques to enhance the models’ performance and applicability in real-world scenarios.
Magnetic Poles Position Detection of Permanent Magnet Linear Synchronous Motor Using Four Linear Hall Effect Sensors
Authors: Zhou Bin , Huang Cong
Published: July 2023

Magnetic pole position detection is the core of the closed-loop control system of the permanent magnet linear synchronous motor (PMLSM), and its position estimation accuracy directly affects control performance and dynamic response speed. In order to solve the problem of the increased estimation error of magnetic pole position caused by magnetic field distortion at the end of PMLSM while also considering the cost of control hardware, the paper uses four linear Hall sensors as magnetic pole position detection components and adopts an optimized estimation algorithm to improve the dynamic performance of the motor. Firstly, a numerical simulation of the magnetic field of poles was conducted using Ansoft Maxwell software, and combined with theoretical analysis, the optimal installation position range of four linear Hall orthogonal placements relative to the motor was obtained. Meanwhile, based on the existing vector tracking position observer, an improved observer detection model is proposed. The Matlab/Simulink software was used to compare the Hall-based detection model with the Hall-based improved observer detection model, verifying the feasibility of the improved detection algorithm. Finally, the rationality of the spatial layout design of linear Hall and the feasibility of improving the estimation algorithm were verified through experiments.
Attitude Tracking Adaptive Control of a Geocentric Polar Displaced Solar Sail
Authors: Zhang Tengfei , Mu Rongjun
Published: July 2023

To deal with the attitude tracking control problem of a struck or pierced geocentric polar displaced solar sail (GPDSS), an attitude adaptive control strategy is proposed in this paper under the complex conditions of unknown inertial parameters, external disturbance and input saturation. First, on the basis of a flexible solar sail spacecraft attitude dynamics model with damping terms and vibration initial values, an integrated disturbance term, including inertial parameter uncertainties and external disturbance, is constructed. Second, a radial basis function neural network is applied to design a disturbance estimator with an adaptive law to estimate the integrated disturbance in real time. Then, a sliding-mode controller with fixed-time convergence in the reach phase and finite-time stability in the sliding phase is designed, and stability analysis is conducted by using the Lyapunov theory. Finally, comparative simulations with a linear sliding-mode controller and numerical simulations under various workings are performed. The results show that the designed adaptive control strategy can effectively achieve the attitude tracking control of the GPDSS.
Reduction of Typical Diesel NOx Emissions by SCR-NH3 Using Metal-Exchanged Natural Zeolite and SBA-15 Catalysts
Authors: Alcantara Amanda Pontes Maia Pires , Moura de Oliveira Mona Lisa , Santiago de Araújo Jesuína Cássia , dos Santos Araújo Rinaldo , Chaves de Lima Rita Karolinny , Bueno André Valente , Vieira da Silva Maria Eugênia , Costa Rocha Paulo Alexandre , Rodríguez-Castellón Enrique
Published: July 2023

In this work, the catalytic performance of clinoptilolite (CLIN) and SBA-15 catalysts, doped with Fe and Cu, was evaluated in the selective catalytic reduction of NO using NH3 as a reducing agent (SCR-NH3). Both Cu-CLIN and Fe-CLIN were obtained by ion-exchange using natural clinoptilolite zeolite originating from the Hrabovec deposit (northeast Slovakia region). Cu-SBA-15 and Fe-SBA-15 were prepared by impregnation into SBA-15 mesoporous synthesized silica. Standard catalytic activity tests were carried out on a bench-scale laboratory apparatus using a reaction mixture of a standard test. GHSV of 48,000 h−1 was adopted based on the space velocity of a real NH3-SCR catalyst for diesel vehicles (100–550 °C). All Cu-doped samples showed better NO conversion values than Fe-doped samples. Clinoptilolite catalysts were more active than those based on SBA-15. Maximum NO conversions of about 96% were observed for Cu-CLIN and Fe-CLIN at 350–400 °C, respectively. Moreover, Fe-CLIN also showed higher stability in the presence of SO2 and water steam at 350 °C. These results demonstrate the potential of metal-doped natural clinoptilolite to be used as cost-effective catalysts applied to the abatement of NOx emissions generated in automotive combustion processes.
Effects of Bacillus licheniformis on the Growth Performance, Antioxidant Capacity, Ileal Morphology, Intestinal Short Chain Fatty Acids, and Colonic Microflora in Piglets Challenged with Lipopolysaccharide
Authors: Cao Guangtian , Yang Shenglan , Wang Huixian , Zhang Ruiqiang , Wu Yanping , Liu Jinsong , Qiu Kaifan , Dong Yingkun , Yue Min
Published: July 2023

The aim of the present study was to investigate the effects of Bacillus licheniformis (BL) on the growth performance, antioxidant capacity, ileal morphology, intestinal fecal short-chain fatty acids, and microflora of weaned piglets challenged with lipopolysaccharide (LPS). Piglets were assigned into three groups: basal diet (Con), a basal diet with added 109 CFU B. licheniformis/kg (BLl), and a basal diet with added 1010 CFU B. licheniformis/kg (BLh). On day 28, BLh piglets were intraperitoneally injected with LPS (CBL) and sterilized saline water (BL), Con piglets were injected with LPS (LPS) and sterilized saline water (Con), with the injections being administered for three consecutive days. The average daily gain significantly increased from day 1 to day 28 and the feed: gain ratio decreased with BL supplementation compared with the Con group. Supplementation with BLl and BLh reduced the diarrhea rate in piglets. Serum catalase activity increased and malondialdehyde concentration decreased in the CBL treatment group compared with the LPS treatment group. Both BL and CBL treatments increased the ileal villus length/crypt depth ratio compared with Con and LPS treatments. BL administration significantly increased colonic propionic and isobutyric acid concentrations compared with Con treatment. Both BL and CBL piglets had significantly increased fecal acetic, propionic, and butyric acid levels compared with LPS piglets. Analysis of the colonic microbial metagenome showed that Prevotella species were the predominant bacteria in piglets treated with BL and CBL. The CBL-treated piglets had higher scores for lysine biosynthesis, arginine biosynthesis, sulfur relay system, and histidine metabolism. BL-treated piglets had higher scores for glycosaminoglycan biosynthesis-keratan sulfate, oxidative phosphorylation, and pyruvate and carbon metabolism.
A Novel Standardized Method for Aiding to Determine Left Atrial Enlargement on Lateral Thoracic Radiographs in Dogs
Authors: Szatmári Viktor , Hofman Zelie M. M. , van Bijsterveldt Nynke J. , Tellegen Anna R. , Vilaplana Grosso Federico R.
Published: July 2023

Background: Left atrial enlargement indicates severe cardiac disease. Although the gold standard for determining left atrial size is echocardiography, many veterinary practices lack the necessary equipment and expertise. Therefore, thoracic radiography is often used to differentiate cardiogenic pulmonary edema from primary respiratory diseases and to facilitate distinguishing dogs with stage B1 and B2 mitral valve degeneration. Methods: The goal was to test a new standardized method for identifying radiographic left atrial enlargement. On a lateral radiograph, a straight line was drawn from the dorsal border of the tracheal bifurcation to the crossing point of the dorsal border of the caudal vena cava and the most cranial crus of the diaphragm. If a part of the left atrium extended this line dorsally, it was considered enlarged. Echocardiographic left atrial to aortic ratio (LA:Ao) was used as a reference. Thirty-nine observers with various levels of experience evaluated 90 radiographs, first subjectively, then applying the new method. Results: The new method moderately correlated with LA:Ao (r = 0.56–0.66) in all groups. The diagnostic accuracy (72–74%) of the subjective assessment and the new method showed no difference. Conclusions: Though the new method was not superior to subjective assessment, it may facilitate learning and subjective interpretation.
Translation Enhancement by a Short Nucleotide Insertion at 5′UTR: Application to an In Vitro Cell-Free System and a Photosynthetic Bacterium
Authors: Kondo Tomo , Shimizu Takayuki
Published: July 2023

We previously showed that insertion of Dictyostelium gene sequences, such as mlcR, upstream of the Shine–Dalgarno sequence, positively impacts downstream gene expression in Escherichia coli. However, the mechanism by which protein production is facilitated and its applicability to other bacteria remains unknown. In this study, a translation-enhancing effect, associated with this system, on the mRNA amount and property as well as the versatility of the method has been demonstrated. The insertion of mlcR-terminal 25 bp (mlcR25) stabilized the mRNAs and led to increased mRNA levels in E. coli. In the in vitro translation system, a four-fold enhancement was observed when DNA was used as the template, and a three-fold enhancement was observed when mRNA was used as the template. This suggests that mlcR25 has an effect on the facilitation of the interaction between mRNA and ribosome. Furthermore, when this enhancement system was adapted to the photosynthetic bacterium Rhodobacter capsulatus, a more than six-fold increase in translation was observed. Thus, we propose that enhanced translation by mlcR25 is mediated by mechanisms that help the translation machinery to work efficiently, and the system can be applied to bacteria other than E. coli.