bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on Spatio-Temporal Changes of Land Use Sustainability in Southwestern Border Mountainous Provinces in Recent 20 Years Based on Remote Sensing Interpretation: A Case Study in Yunnan Province, China
Authors: Yang Renyi , Wu Qiuju , Yang Zisheng , Yang Shiqin
Published: November 2022

The basic characteristics of mountain areas are that the innate ecological environment is relatively fragile, the acquired ecological environment is severely damaged and degraded, the whole economy and society are still relatively backward, and the degree of sustainable development is relatively low. At present, it has not been seen that land use sustainability evaluation (LUSE) research lasting for many years based on RS and GIS in mountainous provinces. It is urgent to build a suitable, feasible, operable and applicable LUSE system for mountainous regions so as to provide a basic foundation for strategic planning and management of sustainable land use in mountainous provinces. This study integrated “RS and GIS + multi-phase LUCC + LUSE theory and quantitative practical methods” based on mountain provinces, firmly grasped the basic characteristics of mountain areas—the vulnerability of ecological environment, and conducted the study on mountain LUSE. According to the outcomes of RS of land use in the study area in three phases (2000, 2010 and 2020), a comprehensive indicator system is established from the three dimensions of ecological friendliness of land use (EFLU), the economic viability of land use (EVLU) and social acceptability of land use (SALU). Using the Delphi method to determine weights and combining the AHP method and the comprehensive analysis method, this paper quantitatively measures the overall sustainability of land use (OSLU) of 129 counties in Yunnan province in 2000, 2010 and 2020, revealing the spatio-temporal characteristics of the OSLU of Yunnan province and all counties from 2000 to 2020, and aiming to provide a basic and foundation for strategic planning and management of sustainable land use (SLU) in mountainous provinces. The degree of ecological friendliness (DEF), degree of economic viability (DEV), degree of social acceptability (DSA) and degree of overall sustainability (DOS) in Yunnan province and all counties have been significantly improved. Accordingly, the OSLU has been improved to a certain extent, with a 0.66% annual growth rate. It indicates that the development and construction from 2000 to 2020 have significantly improved the OSLU. However, regional differences are large. At present, EFLU, EVLU, and SALU in Yunnan province are not high as a whole. The basic situation reflected that there are obvious deficiencies in EFLU, EVLU and SALU in Yunnan province. Land exploitation and use activities have caused certain influences and the destruction of the ecological environment.
Ultrasonographic Evaluation of Sub-Clinical Synovitis in Juvenile Idiopathic Arthritis: The Disease Classification and Management
Authors: Suhaib Rather , Riaz Rasool , Haris Shamas , Mushtaq Bhat , Irfan Robbani , Omair Shah , Ajaz Khushdil , Shehjar Faheem , El-Serehy Hamed
Published: November 2022

Background: Ultrasonography (USG) is a perfect device for analyzing more than one joint in rather brief intervals of time and is well accepted by children with no harmful ionizing radiation, usually does not require sedation, and can be carried out without difficulty in an outpatient setting. Purpose: To demonstrate the ability of ultrasonography (USG) in detecting clinical and subclinical synovitis in children with juvenile idiopathic arthritis (JIA) and compare the USG findings with clinical findings. Methods: 20 patients with JIA diagnosed according to the ILAR criteria were include. A total of 208 joints were examined both clinically and ultrasonographically for detection of synovitis. The presence of subclinical synovitis detected by USG was sought and its effect on the classification of JIA was assessed. USG assessment was done using the High-Resolution Linear probe including both grey scale and Power Doppler assessment. Results: The mean age of patients was 10.2 years with average disease duration of 5.9 months. A total of 49 joints (23.5%) had clinical synovitis and 59 joints (28.4%) had USG synovitis out of a total of 208 joints. A total of 14 joints had subclinical synovitis (8.8% out of the 159 clinically normal joints) upon USG. USG additionally brought about classifying three patients as having poly articular disorder who had been considered as oligo articular upon clinical examination. Conclusion: USG assessment of subclinical synovitis in JIA patients is an essential component of classifying the disease and detects more joints with synovitis than clinical examination; however, both are complimentary and should be used in combination in all patients with JIA.
Alpinumisoflavone Exhibits the Therapeutic Effect on Prostate Cancer Cells by Repressing AR and Co-Targeting FASN- and HMGCR-Mediated Lipid and Cholesterol Biosynthesis
Authors: Basavaraj Praveenkumar , Ruangsai Phakkhathorn , Hsieh Po-Fan , Jiang Wen-Ping , Bau Da-Tian , Huang Guan-Jhong , Huang Wen-Chin
Published: November 2022

Prostate cancer (PCa) is the most common cancer in men, and this has been mainly noticed in Western and Asian countries. The aggregations of PCa and castration-resistant PCa (CRPC) progression are the crucial causes in the mortality of patients without the effective treatment. To seek new remedies for the lethal PCa diseases is currently an urgent need. In this study, we endeavored to investigate the therapeutic efficacy of alpinumisoflavone (AIF), a natural product, in PCa. LNCaP (androgen- sensitive) and C4-2 (CRPC) PCa cells were used. An MTT-based method, soft agar colony forming assay, biological progression approaches were applied to determine cell viability, migration, and invasion. A fatty acid quantification kit, a cholesterol detection kit and oil red O staining were conducted to analyze the intracellular levels of lipids and cholesterols. Apoptosis assays were also performed. AIF reduced cell viability, migration, and invasion in PCa cells. The expression of androgen receptor (AR), fatty acid synthase (FASN), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) was substantially inhibited by AIF treatment in PCa cells. Furthermore, by inhibiting FASN and HMGCR expression, AIF decreased the amounts of intracellular fatty acids, cholesterols, and lipid droplets in PCa cells. Significantly, through coordinated targeting FASN- and HMGCR-regulated biosynthesis and the AR axis, AIF activated the caspase-associated apoptosis in PCa cells. These results collectively demonstrated for the first time the potential of AIF as a novel and attractive remedy and provided an alternative opportunity to cure PCa malignancy.
Genotype × Environment Interaction and Stability Analysis of Commercial Hybrid Grain Corn Genotypes in Different Environments
Authors: Adham Azmi , Ghaffar Mohamad Bahagia Ab , Ikmal Asmuni Mohd , Shamsudin Noraziyah Abd Aziz
Published: November 2022

The introduction of superior grain corn genotypes with high and stable yield (YLD) in most environments is important to increase local production and reduce dependency on imported grain corn. In this study, days to tasseling (DT), plant height, and YLD of 11 grain corn genotypes were observed in 10 environments to evaluate the effects of genotype (G), environment (E), and genotype by environment interactions (GEI) using GGE analysis and the stability of genotypes using stability parameters. In each location, grain corn genotypes were arranged in three replications using a randomized complete block design. An analysis of variance showed that all three traits were highly significant toward G and E factors, whereas GEI showed that only DT and YLD were highly significant. Genotype V14 produced the highest YLD of 10,354 kg/ha, followed by V4 (10,114 kg/ha) and V2 (9797.74 kg/ha). These three genotypes also dominated in seven out of 10 tested environments. With regard to stability ranking, genotype V4 was the most stable genotype, with a big gap difference between the second (V14) and third places (V2). Therefore, V14, V4, and V2 were the most promising genotypes because of their great YLD performance and most stable across tested environments, which can be recommended to farmers for high-scale planting.
Genome-Wide Identification of LeBAHDs in Lithospermum erythrorhizon and In Vivo Transgenic Studies Confirm the Critical Roles of LeBAHD1/LeSAT1 in the Conversion of Shikonin to Acetylshikonin
Authors: Wang Xuan , He Zhuoyu , Yang Huan , He Cong , Wang Changyi , Fazal Aliya , Lai Xiaohui , Yang Liangjie , Wen Zhongling , Yang Minkai , Ma Shenglin , Jie Wencai , Cai Jinfeng , Yin Tongming , Liu Bao , Yang Yonghua , Qi Jinliang
Published: November 2022

The BAHD acyltransferase family is a unique class of plant proteins that acylates plant metabolites and participates in plant secondary metabolic processes. However, the BAHD members in Lithospermum erythrorhizon remain unknown and uncharacterized. Although the heterologously expressed L. erythrorhizon BAHD family member LeSAT1 in Escherichia coli has been shown to catalyze the conversion of shikonin to acetylshikonin in vitro, its in vivo role remains unknown. In this study, the characterization, evolution, expression patterns, and gene function of LeBAHDs in L. erythrorhizon were explored by bioinformatics and transgenic analysis. We totally identified 73 LeBAHDs in the reference genome of L. erythrorhizon. All LeBAHDs were phylogenetically classified into five clades likely to perform different functions, and were mainly expanded by dispersed and WGD/segmental duplication. The in vivo functional investigation of the key member LeBAHD1/LeSAT1 revealed that overexpression of LeBAHD1 in hairy roots significantly increased the content of acetylshikonin as well as the conversion rate of shikonin to acetylshikonin, whereas the CRISPR/Cas9-based knockout of LeBAHD1 in hairy roots displayed the opposite trend. Our results not only confirm the in vivo function of LeBAHD1/LeSAT1 in the biosynthesis of acetylshikonin, but also provide new insights for the biosynthetic pathway of shikonin and its derivatives.
Liver Fibrosis, Liver Cancer, and Advances in Therapeutic Approaches
Authors: Rajapaksha Indu
Published: November 2022

Chronic liver diseases (CLDs) that lead to hepatic fibrosis, cirrhosis, and/or hepatocellular carcinoma (HCC) have become a major cause of illness and death worldwide. The main causative factors for CLDs are chronic viral infections, excessive alcohol consumption, non-alcoholic fatty liver disease (NAFLD), and cholestatic diseases. The primary approach to managing cirrhosis should be removing the causative agent, and the secondary approach should address fibrogenesis. Liver cancer is also a leading cause of death worldwide, and many therapeutic approaches exist to treat the disease. However, liver transplantation remains the last treatment option for cirrhosis and liver cancer. Thus, this review discusses the pathophysiology of liver fibrosis, its progression to cirrhosis and HCC, and current therapeutic options available to treat the diseases with potential therapeutic options that will be available in the near future.
Corrosion Behavior of AZ31B Magnesium Alloy Anode by Sulfate-Reducing Prokaryotes in the Tidal Flat Mud with Different Water Contents
Authors: Li Jinrong , Zhang Ruiyong , Sand Wolfgang , Zhu Qingjun , Liu Xin , Duan Jizhou , Hou Baorong , Zhang Jie
Published: November 2022

At present, there are few studies on microbial corrosion of magnesium anode materials that provide protection for oil pipelines in tidal flat environment. In view of an abnormal failure of magnesium anodes in oil pipelines in a tidal flat mud environment, the influence of the change in water content in the beach mud on the corrosion of AZ31B magnesium anode by sulfate-reducing prokaryote (SRP) was investigated by electrochemical methods, weight loss and surface analysis techniques. SRP can grow well in a tidal flat mud environment and cause microbial corrosion of magnesium alloy. The results show that with the increase in water content, the number of SRP cells in the mud increased, that the corrosivity of tidal flat muds was enhanced and that the corrosion rate of AZ31B magnesium anode was accelerated: compared with the corrosion rate of 0.554 mm/y in 40% water content, the corrosion rate of magnesium alloy samples in 60% water content is as high as 1.38 mm/y.
Bionic Hovering Micro-Aerial Vehicle Using Array-Spiracle Wings
Authors: Zhou Xiangcong , Song Xiaogang , Zhang Deyuan , Liu Yanqiang
Published: November 2022

Hovering ability is the basis of fixed-point monitoring and tracking of an aircraft. Herein, we propose a new hovering vehicle inspired by bird feathers, with an airfoil composed of passive opening and closing feather units. Two wings of the prototype are vertically distributed and driven in anti-phase linear reciprocation. The lift is generated by the asymmetric flow of air caused by the opening and closing of the feather unit. The design of the vehicle is simple and can be incorporated into a large-area hovering platform for large loads. In this paper, the design and fabrication of the vehicle are described, along with an aerodynamic theoretical model of the vehicle kinematics. The correctness of the model was verified by numerical calculations and tests on the opening and closing characteristics of the plume unit. The opening and closing characteristics of the feather unit were investigated and revealed through controlled-variable experiments. An experimental prototype with a mass of 52 g was built. During preliminary flight tests, the vibration frequency was controlled to verify that the prototype was capable of hovering and vertical takeoff and landing.
Design and Evaluation of a Low-Cost Electromechanical System to Test Dynamic Performance of Force Sensors at Low Frequencies
Authors: Esposito Daniele , Centracchio Jessica , Andreozzi Emilio , Bifulco Paolo , Gargiulo Gaetano D.
Published: November 2022

Piezoresistive or piezoelectric force sensors are widely available today. These sensors are preferred to loadcells because of their extremely reduced size, slimness, and low cost, which allow their easy inclusion in a large variety of devices including wearables. In particular, many applications are devoted to monitoring human body movements, such as those related to breathing, muscle contraction, walking, etc. However, such sensors offer variable performance, and they need to be individually calibrated and tested to ensure accurate measurements. An automated electromechanical system that allows simple mechanical tests of force sensors is proposed. The system by means of an electrical motor; a gear box; a connecting rod-crank mechanism; two pistons, and a coupling spring between them, impress sinusoidal axial forces onto the sensor under test. The system is designed as modular so that it can be customized: the force range to which the sensor is subjected, the frequency range, and the coupler with the sensor can be changed to resemble the actual application context. The actual force (read from a loadcell coupled to the sensor under test), a piston displacement, and the sensor output are simultaneously recorded. The electromechanical system generates nearly pure sinusoidal stresses at varying low frequencies (mean total harmonic distortion of 2.77%). The energy dissipated for a single stress cycle was 3.62 gf mm on average. The developed system was used to test a Force Sensitive Resistor (FSR)-based sensor and a piezoelectric (PZT) sensor. The tests revealed significant differences from the actual force values (particularly at very low frequencies), output drifts of the FSR sensor in measurements, and non-linear behaviors. The system was found to be able to provide dynamic performances, accurate calibration, and non-linear behavior of the individual sensor.
Early Fault Warning Method of Wind Turbine Main Transmission System Based on SCADA and CMS Data
Authors: Chen Huanguo , Chen Jie , Dai Juchuan , Tao Hanyu , Wang Xutao
Published: November 2022

The main transmission system of wind turbines is a multi-component coupling system, and its operational state is complex and varied. These lead to frequent false alarms and missed alarms in existing monitoring systems. To accurately obtain the operational state of the main transmission system and detect its abnormal operation, an early fault warning method for the main transmission system based on SCADA and CMS data is proposed. Firstly, the SCADA and CMS feature parameters relevant to the operating status of the main transmission system are selected by two different methods separately, and the correlation mechanism between the feature parameters and the operating characteristics of the main transmission system is further analyzed. Secondly, the Long Short-Term Memory (LSTM) network-based prediction model of the main transmission system operating parameters is established, in which SCADA and CMS feature parameters are fused as the input feature vectors. Then, the predicted residuals of the state evaluation parameters are used as the operational state evaluation index. The early fault warning model is established by Analytic Hierarchy Process (AHP) and Kernel Density Estimation (KDE). Finally, a case study is used to verify the correct performance of the proposed method. The results show that this method can realize early warning functions 73 h earlier than the existing SCADA system. The method can provide a theoretical basis for the safe operation and condition-based maintenance of wind turbines.
Optimization of Gain Scheduled Controller for an Active Trailer Steering System Using an Evolutionary Algorithm
Authors: Qureshi Khizar , Liscano Ramiro , He Yuping
Published: November 2022

Car–trailer combinations can experience unstable motion modes such as trailer-sway, jackknifing and rollover that can lead to fatal accidents. These unstable motions can be mitigated with the use of an active trailer steering (ATS) system. Prior studies in ATS have leveraged the linear quadratic regulator (LQR) as an ATS controller but for many of these designs it was assumed that the vehicle and operating parameters were constant. In reality, vehicle and operating parameters may vary and have an impact on the stability of a car–trailer combination. In this paper, the weighting matrices of the LQR controller are determined using the GDE3 evolutionary optimization algorithm with the objective of addressing the design trade off between minimizing the car–trailer’s path-following performance for low vehicle speeds and minimizing the rearward amplification for high vehicle speeds. The effectiveness of the approach is demonstrated using a numerical simulation car–trailer model developed in the CarSim simulator. Our results show that the multi-objective tuned gain scheduling controller outperforms a non-tuned gain scheduling controller in terms of improving the lateral stability and the path following performance of car–trailer combinations in driver in the loop single lane-change maneuvers at a constant vehicle forward speed.
Spheres and Tori as Elliptic Linear Weingarten Surfaces
Authors: Kim Dong-Soo , Kim Young Ho , Qian Jinhua
Published: November 2022

The linear Weingarten condition with ellipticity for the mean curvature and the extrinsic Gaussian curvature on a surface in the three-sphere can define a Riemannian metric which is called the elliptic linear Weingarten metric. We established some local characterizations of the round spheres and the tori immersed in the 3-dimensional unit sphere, along with the Laplace operator, the spherical Gauss map and the Gauss map associated with the elliptic linear Weingarten metric.
Optimal Control of Degrading Units through Threshold-Based Control Policies
Authors: Efrosinin Dmitry , Stepanova Natalia
Published: November 2022

Optimal control problems are applied to a variety of dynamical systems with a random law of motion. In this paper we show that the random degradation processes defined on a discrete set of intermediate degradation states are also suitable for formulating and solving optimization problems and finding an appropriate optimal control policy. Two degradation models are considered in this paper: with random time to an instantaneous failure and with random time to a preventive maintenance. In both cases, a threshold-based control policy with two thresholds levels defining the signal state, after which an instantaneous failure or preventive maintenance can occur after a random time, and a maximum number of intermediate degradation states is applied. The optimal control problem is mainly solved in a steady-state regime. The main loss functional is formulated as the average cost per unit of time for a given cost structure. The Markov degradation models are used for numerical calculations of the optimal threshold policy and reliability function of the studied degrading units.
Clinical and Prognostic Values of TRPM7 in Colon and Rectal Cancers
Authors: Lee Jun-Chae , Bae An-Na , Lee Ha-Jin , Lee Jae-Ho
Published: November 2022

Background and Objectives: Transient receptor potential melastatin 7 (TRPM7) is a unique channel protein, and functionally responsible for transportation of calcium and magnesium. Physiologically, the TRPM7 channel is involved in homeostasis of calcium and magnesium, and cell survival. TRPM7 expression is up-regulated in many cancers as malignant behaviors of cancer cells, and its deficiency suppresses their growth. Materials and Methods: In this study, we aimed to analyze clinical and prognostic characteristics of TRPM7 expression in colorectal cancers (CRC) using The Cancer Genome Atlas. Results: High expression of TRPM7 was observed in younger patients with rectal cancer (p = 0.0002). By quantitative correlation analysis, TRPM7 was negatively correlated with age (R = −0.239, p = 0.003) and p53 (R = −0.240, p = 0.002). Furthermore, it was positively correlated with APC expression (R = 0.534, p < 0.001) and KRAS expression (R = 0.319, p < 0.001). In colon cancer, there were no variables that showed a significant correlation with TRPM7. Survival analysis found that TRPM7 expression did not have any prognostic value in colon and rectal cancers. Conclusions: Our study highlights that TRPM7 expression in CRC, particularly in rectal cancer, may be a potential marker. Future studies are needed to provide deeper insights into the role of TRPM7 in rectal cancer.
Plasma Metabolome Alterations Discriminate between COVID-19 and Non-COVID-19 Pneumonia
Authors: More Tushar H. , Mozafari Bahareh , Märtens Andre , Herr Christian , Lepper Philipp M. , Danziger Guy , Volk Thomas , Hoersch Sabrina , Krawczyk Marcin , Guenther Katharina , Hiller Karsten , Bals Robert
Published: November 2022

Pneumonia is a common cause of morbidity and mortality and is most often caused by bacterial pathogens. COVID-19 is characterized by lung infection with potential progressive organ failure. The systemic consequences of both disease on the systemic blood metabolome are not fully understood. The aim of this study was to compare the blood metabolome of both diseases and we hypothesize that plasma metabolomics may help to identify the systemic effects of these diseases. Therefore, we profiled the plasma metabolome of 43 cases of COVID-19 pneumonia, 23 cases of non-COVID-19 pneumonia, and 26 controls using a non-targeted approach. Metabolic alterations differentiating the three groups were detected, with specific metabolic changes distinguishing the two types of pneumonia groups. A comparison of venous and arterial blood plasma samples from the same subjects revealed the distinct metabolic effects of pulmonary pneumonia. In addition, a machine learning signature of four metabolites was predictive of the disease outcome of COVID-19 subjects with an area under the curve (AUC) of 86 ± 10%. Overall, the results of this study uncover systemic metabolic changes that could be linked to the etiology of COVID-19 pneumonia and non-COVID-19 pneumonia.
Circularly Polarized MIMO Antenna with Wideband and High Isolation Characteristics for C-Band Communication Systems
Authors: Hussain Niamat , Pham Tuyen , Tran Huy-Hung
Published: November 2022

This paper presents a circularly polarized (CP) multiple-input multiple-output (MIMO) antenna using a microstrip patch and parasitic elements. The proposed design exhibits wideband characteristics for both impedance and axial ratio bandwidths. Especially, the mutual coupling between the MIMO elements is significantly depressed without using any decoupling network. To achieve these features, parasitic elements are positioned nearby and in different layers to the radiating elements. The measured results demonstrate that the proposed MIMO CP antenna has a wideband operation of 11.3% (5.0–5.6 GHz), which is defined by an overlap between −10–dB impedance and 3–dB axial ratio bandwidths. Across this band, the realized gain is better than 6.0 dBi, and the isolation is greater than 32 dB with the highest value of 45 dB. The MIMO parameters such as the envelope correlation coefficient, diversity gain, and channel capacity loss are also investigated thoroughly, which are found to be good on the scale of diversity standards.
Breakage Ratio of Silicon Wafer during Fixed Diamond Wire Sawing
Authors: Liu Tengyun , Su Yancai , Ge Peiqi
Published: November 2022

Monocrystalline silicon is an important material for processing electronic and photovoltaic devices. The fixed diamond wire sawing technology is the first key technology for monocrystalline silicon wafer processing. A systematic study of the relationship between the fracture strength, stress and breakage rate is the basis for thinning silicon wafers. The external vibration excitation of sawing machine and diamond wire lead to the transverse vibration and longitudinal vibration for silicon wafers. The transverse vibration is the main reason of wafer breakage. In this paper, a mathematical model for calculating breakage ratio of silicon wafer is established. The maximum stress and breakage ratio for as-sawn silicon wafers are studied. It is found that the maximum amplitude of the silicon wafers with the size of 156 mm × 156 mm × 0.2 mm was 160 μm during the diamond wire sawing process. The amplitude, maximum stress and breakage rate of the wafers increased with the increase of the cutting depth. The smaller the silicon wafer thickness, the larger of silicon wafer breakage ratio. In the sawing stage, the breakage ratio of the 156 mm × 156 mm section with a thickness of 0.15 mm of silicon wafers is 6%.
Ophthalmic Hydrogel Contact Lens Material Containing Magnesium Oxide Nanoparticles and 3-(Trifluoromethyl)styrene for Biomedical Application
Authors: Lee Min-Jae , Park Seon-Young , Sung A-Young
Published: November 2022

This research was conducted for the synthesis and application of ophthalmic lens materials with improved oxygen permeability and durability. Polyvinylpyrrolidone (PVP), N-vinyl-2-pyrrolidone (NVP), 3-(trifluoromethyl)styrene (3-TFMSt), and magnesium oxide nanoparticles were used as additives for the basic combination of 2-hydroxyethyl methacrylate (HEMA). Additionally, the materials were copolymerized with ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent and azobisisobutyronitrile (AIBN) as the initiator. The addition of magnesium oxide nanoparticles was found to increase the tensile strength from 0.0631 to 0.0842 kgf/mm2. Copolymerization with a small amount of 3-TFMSt of about 1% increased the tensile strength to 0.1506 kgf/mm2 and the oxygen permeability from 6.00 to 9.64 (cm2/s)∙(mLO2/mL·mmHg)∙10−11. The contact lens material produced using N-vinyl-2-pyrrolidone and magnesium oxide nanoparticles as additives satisfied the basic physical properties required for hydrogel contact lenses and is expected to be used usefully as a material for fabricating high-performance hydrogel lenses.
High Sensitivity Planar Hall Effect Magnetic Field Gradiometer for Measurements in Millimeter Scale Environments
Authors: Nhalil Hariharan , Schultz Moty , Amrusi Shai , Grosz Asaf , Klein Lior
Published: November 2022

We report a specially designed magnetic field gradiometer based on a single elliptical planar Hall effect (PHE) sensor, which allows measuring magnetic field at nine different positions in a 4 mm length scale. The gradiometer detects magnetic field gradients with equivalent gradient magnetic noises of ∼958, ∼192, ∼51, and ∼26 nT/m√ Hz (pT/mm√Hz) at 0.1, 1, 10, and 50 Hz, respectively. The performance of the gradiometer is tested in ambient conditions by measuring the field gradient induced by electric currents driven in a long straight wire. This gradiometer is expected to be highly useful for the measurement of magnetic field gradients in confined areas for its small footprint, low noise, scalability, simple design, and low costs.
Cardiovascular Complications in Community-Acquired Pneumonia
Authors: Desai Antonio , Aliberti Stefano , Amati Francesco , Stainer Anna , Voza Antonio
Published: November 2022

Community-acquired pneumonia (CAP) is accountable for high mortality in both pediatric and adult populations worldwide, about one-third of hospitalized patients pass away within a year of being discharged from the facility. The high mortality and morbidity rates are closely related to cardiovascular complications that are consequent or concomitant to the acute episode of pneumonia. An updated perspective on the major pathophysiological mechanisms, prevalence, risk factors, outcomes, and relevant treatments of cardiovascular events in CAP patients is provided in the current study. It is possible to evaluate the pathophysiology of cardiac disease in this population based on plaque-related events, such as acute myocardial infarction, or events unrelated to plaque, such as arrhythmias and heart failure. With an absolute rate of cardiovascular problems ranging broadly from 10% to 30%, CAP raises the risk of both plaque-related and plaque-unrelated events. Both in- and out-patients may experience these issues at admission, throughout hospitalization, or even up to a year following discharge. At long-term follow-up, cardiac events account for more than 30% of deaths in CAP patients, making them a significant cause of mortality. If patients at risk for cardiac events are stratified, diagnostic tools, monitoring, and preventive measures may be applied to these patients. A prospective evaluation of cardioprotective treatments is urgently required from a research point of view.
Mineralization Based on CSAMT and SIP Sounding Data: A Case Study on the Hadamengou Gold Deposit in Inner Mongolia
Authors: Lv Hanqin , Xu Liuyang , Yang Biao , Su Panyun , Xu Haoqing , Wang Hailong , Yao Chuan , Su Peng
Published: November 2022

The Hadamengou deposit is the largest gold deposit in Inner Mongolia. However, given that the sources of ore-forming alkaline magmatic hydrothermal solutions and ore-controlling structures are still controversial, the theories behind the genesis of the deposit have been controversial. In this study, four controlled-source audio magnetotellurics (CSAMT) and spectral induced polarization (SIP) profiles in the mining area were used to obtain the underground resistivity model and the pseudo section map of the apparent frequency dispersivity based on fine inversion. In the resistivity model, there are two high-resistivity blocks with resistivity greater than 3000 Ω m and three low-resistivity channels with resistivity less than 50 Ω m. Combined with the regional geological and drilling data, it is inferred that the high-resistance bodies, R4 and R5, may be alkaline magmatic intrusions related to multiple stages of magmatic hydrothermal activities, ranging from the Precambrian to Yanshanian periods. The highly conductive channels, C3, C5, and C4, may represent the Baotou-Hohhot fault, secondary faults, and ductile shear zone, respectively, which were formed in the Precambrian era and underwent multiple activations during the Hercynian to Yanshanian period. According to the spatial relationship, it is inferred that the ductile shear zone is an important ore-controlling and ore-hosting structure. However, the Baotou–Hohhot fault may be a pre-metallogenic fault rather than an ore-controlling fault. By comparing the resistivity model with the pseudo section of the apparent frequency dispersivity, it was found that all the known gold veins are located in the superimposed area of low resistivity and high-frequency dispersivity. It is speculated that the ductile shear zone outside the alkaline magmatic rock with the superimposed characteristics of low resistivity and high-frequency dispersivity is the favorable area for mineralization.
The Chemical Composition and Antimitotic, Antioxidant, Antibacterial and Cytotoxic Properties of the Defensive Gland Extract of the Beetle, Luprops tristis Fabricius
Authors: Sabira Ovungal , Vignesh Attuvalappil Ramdas , Ajaykumar Anthyalam Parambil , Varma Sudhir Rama , Jayaraj Kodangattil Narayanan , Sebastin Merin , Nikhila Kalleringal , Babu Annet , Rasheed Vazhanthodi Abdul , Binitha Valiyaparambil Sivadasan , Vasu Zeena koldath , Sujith Madathilpadi Subrahmanian
Published: November 2022

The unpredictable invasion of the Mupli beetle, Luprops tristis Fabricius (Coleoptera: Tenebrionidae), makes areas uninhabitable to humans. These beetles produce a strong-smelling, irritating secretion as a defence mechanism, which causes blisters on contact with human skin. In the current study, gas chromatography high-resolution mass spectrometry (GC-HRMS) analysis of the defensive gland extract of the Mupli beetle revealed the presence of compounds such as 2,3,dimethyl-1,4-benzoquinone, 1,3-dihydroxy-2-methylbenzene, 2,5-dimethyl hydroquinone, tetracosane, oleic acid, hexacosane, pentacosane, 7-hexadecenal and tert-hexadecanethiol. The defensive gland extracts showed considerable antibacterial activity on Gram-negative and Gram-positive bacteria in an agar diffusion assay. The chromosomal aberration analysis using root tips of Allium cepa L. exposed to the defensive secretion showed chromosomal aberrations such as disturbed metaphase, sticky chromosomes and chromosomal breakage. The antioxidant activity of the extract was determined using a radical scavenging (DPPH) assay. A cytotoxic assay of the defensive gland extract against Dalton’s lymphoma ascites (DLA) cell line showed anticancer properties. In the present study, the defensive gland extract of the Mupli beetle, L. tristis, which is generally perceived as a nuisance insect to humans, was found to have beneficial biological activities.
Effects of Tebuconazole Application on Soil Microbiota and Enzymes
Authors: Baćmaga Małgorzata , Wyszkowska Jadwiga , Borowik Agata , Kucharski Jan
Published: November 2022

Identification of pesticide impact on the soil microbiome is of the utmost significance today. Diagnosing the response of bacteria to tebuconazole, used for plant protection, may help isolate the most active bacteria applicable in the bioaugmentation of soils contaminated with this preparation. Bearing in mind the above, a study was undertaken to test the effect of tebuconazole on the diversity of bacteria at all taxonomic levels and on the activity of soil enzymes. It was conducted by means of standard and metagenomic methods. Its results showed that tebuconazole applied in doses falling within the ranges of good agricultural practice did not significantly disturb the biological homeostasis of soil and did not diminish its fertility. Tebuconazole was found to stimulate the proliferation of organotrophic bacteria and fungi, and also the activities of soil enzymes responsible for phosphorus, sulfur, and carbon metabolism. It did not impair the activity of urease responsible for urea hydrolysis, or cause any significant changes in the structure of bacterial communities. All analyzed soil samples were mainly populated by bacteria from the phylum Proteobacteria, Actinobacteria, Firmicutes, Gemmatimonadetes, Acidobacteria, Planctomycetes, and Chloroflexi. Bacteria from the genera Kaistobacter, Arthrobacter, and Streptomyces predominated in the soils contaminated with tebuconazole, whereas these from the Gemmata genus were inactivated by this preparation.
Post-Consumer Poly(ethylene terephthalate) (PET) Depolymerization by Yarrowia lipolytica: A Comparison between Hydrolysis Using Cell-Free Enzymatic Extracts and Microbial Submerged Cultivation
Authors: Sales Julio Cesar Soares , de Castro Aline Machado , Ribeiro Bernardo Dias , Coelho Maria Alice Zarur
Published: November 2022

Several microorganisms have been reported as capable of acting on poly(ethylene terephthalate) (PET) to some extent, such as Yarrowia lipolytica, which is a yeast known to produce various hydrolases of industrial interest. The present work aims to evaluate PET depolymerization by Y. lipolytica using two different strategies. In the first one, biocatalysts were produced during solid-state fermentation (SSF-YL), extracted and subsequently used for the hydrolysis of PET and bis(2-hydroxyethyl terephthalate) (BHET), a key intermediate in PET hydrolysis. Biocatalysts were able to act on BHET, yielding terephthalic acid (TPA) (131.31 µmol L−1), and on PET, leading to a TPA concentration of 42.80 µmol L−1 after 168 h. In the second strategy, PET depolymerization was evaluated during submerged cultivations of Y. lipolytica using four different culture media, and the use of YT medium ((w/v) yeast extract 1%, tryptone 2%) yielded the highest TPA concentration after 96 h (65.40 µmol L−1). A final TPA concentration of 94.3 µmol L−1 was obtained on a scale-up in benchtop bioreactors using YT medium. The conversion obtained in bioreactors was 121% higher than in systems with SSF-YL. The results of the present work suggest a relevant role of Y. lipolytica cells in the depolymerization process.
Role of Iron Oxide (Fe2O3) Nanocomposites in Advanced Biomedical Applications: A State-of-the-Art Review
Authors: Pourmadadi Mehrab , Rahmani Erfan , Shamsabadipour Amin , Mahtabian Shima , Ahmadi Mohammadjavad , Rahdar Abbas , Díez-Pascual Ana M.
Published: November 2022

Nanomaterials have demonstrated a wide range of applications and recently, novel biomedical studies are devoted to improving the functionality and effectivity of traditional and unmodified systems, either drug carriers and common scaffolds for tissue engineering or advanced hydrogels for wound healing purposes. In this regard, metal oxide nanoparticles show great potential as versatile tools in biomedical science. In particular, iron oxide nanoparticles with different shape and sizes hold outstanding physiochemical characteristics, such as high specific area and porous structure that make them idoneous nanomaterials to be used in diverse aspects of medicine and biological systems. Moreover, due to the high thermal stability and mechanical strength of Fe2O3, they have been combined with several polymers and employed for various nano-treatments for specific human diseases. This review is focused on summarizing the applications of Fe2O3-based nanocomposites in the biomedical field, including nanocarriers for drug delivery, tissue engineering, and wound healing. Additionally, their structure, magnetic properties, biocompatibility, and toxicity will be discussed.
Effect of Resistant Dextrin on Intestinal Gas Homeostasis and Microbiota
Authors: Barber Claudia , Sabater Carlos , Ávila-Gálvez María Ángeles , Vallejo Fernando , Bendezu Rogger Alvaro , Guérin-Deremaux Laetitia , Guarner Francisco , Espín Juan Carlos , Margolles Abelardo , Azpiroz Fernando
Published: November 2022

Previous studies have shown that a resistant dextrin soluble fibre has prebiotic properties with related health benefits on blood glucose management and satiety. Our aim was to demonstrate the effects of continuous administration of resistant dextrin on intestinal gas production, digestive sensations, and gut microbiota metabolism and composition. Healthy subjects (n = 20) were given resistant dextrin (14 g/d NUTRIOSE®, Roquette Frères, Lestrem, France) for four weeks. Outcomes were measured before, at the beginning, end, and two weeks after administration: anal evacuations of gas during daytime; digestive perception, girth, and gas production in response to a standard meal; sensory and digestive responses to a comfort meal; volume of colonic biomass by magnetic resonance; taxonomy and metabolic functions of fecal microbiota by shotgun sequencing; metabolomics in urine. Dextrin administration produced an initial increase in intestinal gas production and gas-related sensations, followed by a subsequent decrease, which magnified after discontinuation. Dextrin enlarged the volume of colonic biomass, inducing changes in microbial metabolism and composition with an increase in short chain fatty acids-producing species and modulation of bile acids and biotin metabolism. These data indicate that consumption of a soluble fibre induces an adaptative response of gut microbiota towards fermentative pathways with lower gas production.
Association of CYP2R1 and VDR Polymorphisms with Metabolic Syndrome Components in Non-Diabetic Brazilian Adolescents
Authors: Araújo Eduarda Pontes dos Santos , Lima Severina Carla Vieira da Cunha , Galdino Ony Araújo , Arrais Ricardo Fernando , de Souza Karla Simone Costa , de Rezende Adriana Augusto
Published: November 2022

Associations between vitamin D deficiency and metabolic syndrome (MS) have been reported; however, the underlying biological mechanisms remain controversial. The aim of this study was to investigate the associations of CYP2R1 and VDR variants with MS and MS components in non-diabetic Brazilian adolescents. This cross-sectional study included 174 adolescents who were classified as overweight/obese. Three CYP2R1 variants and four VDR variants were identified by allelic discrimination. The CYP2R1 polymorphisms, rs12794714 (GG genotype) (odds ratio [OR] = 3.54, 95% confidence interval [CI] = 1.24–10.14, p = 0.023) and rs10741657 (recessive model—GG genotype) (OR = 3.90, 95%CI = 1.18–12.92, p = 0.026) were significantly associated with an increased risk of MS and hyperglycemia, respectively. The AG + GG genotype (dominant model) of the rs2060793 CYP2R1 polymorphism was associated with hyperglycemia protection (OR = 0.28, 95%CI = 0.08–0.92, p = 0.037). Furthermore, the CC genotype (recessive model) of the rs7975232 VDR polymorphism was significantly associated with a risk of hypertension (OR = 5.91, 95%CI = 1.91–18.32, p = 0.002). In conclusion, the CYP2R1 rs12794714 polymorphism could be considered a possible new molecular marker for predicting the risk of MS; CYP2R1 rs10741657 polymorphism and VDR rs7975232 polymorphism are associated with an increased risk of diabetes and hypertension in adolescents with overweight/obesity.
Protective Effects of Fish (Alaska Pollock) Protein Intake against Short-Term Memory Decline in Senescence-Accelerated Mice
Authors: Murakami Yuki , Hosomi Ryota , Nishimoto Ayano , Nishiyama Toshimasa , Yoshida Munehiro , Fukunaga Kenji
Published: November 2022

Dietary fish intake has proven to have health benefits in humans. n-3 polyunsaturated fatty acids (PUFAs) in fish oil (FO), especially, may provide protection against age-related cognitive disorders. Owing to the unique benefits of n-3 PUFAs, other nutrients, such as fish protein (FP), have not been well studied. To clarify the effects of FO and FP on brain function, we investigated whether FO or FP feeding can prevent age-related cognitive dysfunction in senescence-accelerated mouse-prone 10 (SAMP10) mice. The FP group maintained a better working memory compared to the control and FO groups in the Y-maze test, but not episodic memory in the novel object recognition test. To evaluate demyelination levels, we measured neurofilament H (NfH) and myelin basic protein (MBP) immunoreactivity in the hippocampus (Hipp). Axon morphology was maintained in the FP group, but not in the control and FO groups. Additionally, the percentage of positive area for double-staining with NfH/MPB was significantly higher in the Hipp of FP-fed mice than in the control (p < 0.05). These results suggest that FP intake prevents age-related cognitive dysfunction by maintaining axonal morphology in the Hipp of SAMP10 mice.
The Importance of Nutritional Aspects in the Assessment of Inflammation and Intestinal Barrier in Patients with Inflammatory Bowel Disease
Authors: Kaczmarczyk Olga , Dąbek-Drobny Agnieszka , Piątek-Guziewicz Agnieszka , Woźniakiewicz Michał , Paśko Paweł , Dobrowolska-Iwanek Justyna , Woźniakiewicz Aneta , Targosz Aneta , Ptak-Belowska Agata , Szczyrk Urszula , Strzałka Małgorzata , Zagrodzki Paweł , Zwolińska-Wcisło Małgorzata
Published: November 2022

Intestinal inflammation in inflammatory bowel disease (IBD) is closely linked to nutrition. This study aimed to evaluate associations between nutritional, inflammatory, and intestinal barrier parameters in patients with IBD. We assessed nutritional status, fecal short-chain fatty acid profile, serum cytokine levels, and mRNA expression of enzymes and tight junction proteins in intestinal biopsies obtained from 35 patients, including 11 patients with inactive IBD, 18 patients with active IBD, and six controls. Patients with active IBD were characterized by hypoalbuminemia, fluctuations in body weight, and restriction of fiber-containing foods. In addition, they had significantly reduced levels of isovaleric acid and tended to have lower levels of butyric, acetic, and propionic acids. Patients with active IBD had higher mRNA expression of peroxisome proliferator-activated receptor γ and inducible nitric oxide synthase, and lower mRNA expression of claudin-2 and zonula occludens-1, compared with patients with inactive IBD. Moreover, patients with a body mass index (BMI) of ≥25 kg/m2 had higher median tumor necrosis factor-α levels that those with a lower BMI. We comprehensively evaluated inflammatory parameters in relation to IBD activity and nutritional status. The discrepancies between proinflammatory and anti-inflammatory parameters depending on IBD activity may be related to nutritional factors, including diet and abnormal body weight.
Vitamin D Status and Risk of All-Cause and Cause-Specific Mortality in Osteoarthritis Patients: Results from NHANES III and NHANES 2001–2018
Authors: Wang Jing , Fan Jiayao , Yang Ye , Moazzen Sara , Chen Dingwan , Sun Lingling , He Fan , Li Yingjun
Published: November 2022

Objectives: The role of Vitamin D (VD) in calcium balance and bone health makes VD a vital factor in osteoarthritis (OA). Studies that have evaluated the effect of VD on OA patients have mainly been performed on a short-term basis. In this analysis, we aimed to evaluate whether VD was associated with mortality, a long-term outcome, in OA patients. Methods: Participants with self-reported OA from NHANES III and NHANES 2001–2018 were included. Associations of 25(OH)D concentrations with mortality risk were assessed continuously using restricted cubic splines and by categories (i.e., <25.0, 25.0–49.9, 50.0–74.9, and ≥75.0 nmol/L) using the Cox regression model. Sensitivity and stratified analyses were performed to evaluate the robustness of the results. Results: A total of 4570 patients were included, of which 1388 died by 31 December 2019. An L-shaped association was observed between 25(OH)D concentrations and all-cause mortality, whereas an inverse association was found for cardiovascular disease (CVD) mortality. The adjusted hazard ratios (95% confidence intervals) across four categories were 1.00 (reference), 0.49 (0.31, 0.75), 0.45 (0.29, 0.68), and 0.43 (0.27, 0.69) for all-cause mortality and 1.00 (reference), 0.28 (0.14, 0.59), 0.25 (0.12, 0.51), and 0.24 (0.11, 0.49) for CVD-specific mortality; no significant associations were found for cancer-specific mortality. Similar results were observed when stratified and sensitivity analyses were performed. Conclusions: Compared with patients with insufficient or deficient serum 25(OH)D, those with sufficient 25(OH)D concentrations had a lower risk of all-cause and CVD mortality, supporting a beneficial role of VD on a long-term basis.