bluuebunny/crossref_metadata_2025 huggingface dataset:
RETRACTED: CAR-NK Cell: A New Paradigm in Tumor Immunotherapy
Authors: Marofi Faroogh , Al-Awad Alaa S. , Sulaiman Rahman Heshu , Markov Alexander , Abdelbasset Walid Kamal , Ivanovna Enina Yulianna , Mahmoodi Mahnaz , Hassanzadeh Ali , Yazdanifar Mahboubeh , Stanley Chartrand Max , Jarahian Mostafa
Published: June 2021

The tumor microenvironment (TME) is greatly multifaceted and immune escape is an imperative attribute of tumors fostering tumor progression and metastasis. Based on reports, the restricted achievement attained by T cell immunotherapy reflects the prominence of emerging other innovative immunotherapeutics, in particular, natural killer (NK) cells-based treatments. Human NK cells act as the foremost innate immune effector cells against tumors and are vastly heterogeneous in the TME. Currently, there exists a rapidly evolving interest in the progress of chimeric antigen receptor (CAR)-engineered NK cells for tumor immunotherapy. CAR-NK cells superiorities over CAR-T cells in terms of better safety (e.g., absence or minimal cytokine release syndrome (CRS) and graft-versus-host disease (GVHD), engaging various mechanisms for stimulating cytotoxic function, and high feasibility for ‘off-the-shelf’ manufacturing. These effector cells could be modified to target various antigens, improve proliferation and persistence in vivo, upturn infiltration into tumors, and defeat resistant TME, which in turn, result in a desired anti-tumor response. More importantly, CAR-NK cells represent antigen receptors against tumor-associated antigens (TAAs), thereby redirecting the effector NK cells and supporting tumor-related immunosurveillance. In the current review, we focus on recent progress in the therapeutic competence of CAR-NK cells in solid tumors and offer a concise summary of the present hurdles affecting therapeutic outcomes of CAR-NK cell-based tumor immunotherapies.
Cellular Metabolomics Profiles Associated With Drug Chemosensitivity in AML
Authors: Stockard Bradley , Bhise Neha , Shin Miyoung , Guingab-Cagmat Joy , Garrett Timothy J. , Pounds Stanley , Lamba Jatinder K.
Published: June 2021

Background

Acute myeloid leukemia (AML) is a hematological malignancy with a dismal prognosis. For over four decades, AML has primarily been treated by cytarabine combined with an anthracycline. Although a significant proportion of patients achieve remission with this regimen, roughly 40% of children and 70% of adults relapse. Over 90% of patients with resistant or relapsed AML die within 3 years. Thus, relapsed and resistant disease following treatment with standard therapy are the most common clinical failures that occur in treating this disease. In this study, we evaluated the relationship between AML cell line global metabolomes and variation in chemosensitivity.

Methods

We performed global metabolomics on seven AML cell lines with varying chemosensitivity to cytarabine and the anthracycline doxorubicin (MV4.11, KG-1, HL-60, Kasumi-1, AML-193, ME1, THP-1) using ultra-high performance liquid chromatography – mass spectrometry (UHPLC-MS). Univariate and multivariate analyses were performed on the metabolite peak intensity values from UHPLC-MS using MetaboAnalyst to identify cellular metabolites associated with drug chemosensitivity.

Results

A total of 1,624 metabolic features were detected across the leukemic cell lines. Of these, 187 were annotated to known metabolites. With respect to doxorubicin, we observed significantly greater abundance of a carboxylic acid (1-aminocyclopropane-1-carboxylate) and several amino acids in resistant cell lines. Pathway analysis found enrichment of several amino acid biosynthesis and metabolic pathways. For cytarabine resistance, nine annotated metabolites were significantly different in resistance vs. sensitive cell lines, including D-raffinose, guanosine, inosine, guanine, aldopentose, two xenobiotics (allopurinol and 4-hydroxy-L-phenylglycine) and glucosamine/mannosamine. Pathway analysis associated these metabolites with the purine metabolic pathway.

Conclusion

Overall, our results demonstrate that metabolomics differences contribute toward drug resistance. In addition, it could potentially identify predictive biomarkers for chemosensitivity to various anti-leukemic drugs. Our results provide opportunity to further explore these metabolites in patient samples for association with clinical response.
Competing Endogenous RNA in Colorectal Cancer: An Analysis for Colon, Rectum, and Rectosigmoid Junction
Authors: Vieira Lucas Maciel , Jorge Natasha Andressa Nogueira , de Sousa João Batista , Setubal João Carlos , Stadler Peter F. , Walter Maria Emília Machado Telles
Published: June 2021

Background

Colorectal cancer (CRC) is a heterogeneous cancer. Its treatment depends on its anatomical site and distinguishes between colon, rectum, and rectosigmoid junction cancer. This study aimed to identify diagnostic and prognostic biomarkers using networks of CRC-associated transcripts that can be built based on competing endogenous RNAs (ceRNA).

Methods

RNA expression and clinical information data of patients with colon, rectum, and rectosigmoid junction cancer were obtained from The Cancer Genome Atlas (TCGA). The RNA expression profiles were assessed through bioinformatics analysis, and a ceRNA was constructed for each CRC site. A functional enrichment analysis was performed to assess the functional roles of the ceRNA networks in the prognosis of colon, rectum, and rectosigmoid junction cancer. Finally, to verify the ceRNA impact on prognosis, an overall survival analysis was performed.

Results

The study identified various CRC site-specific prognosis biomarkers: hsa-miR-1271-5p, NRG1, hsa-miR-130a-3p, SNHG16, and hsa-miR-495-3p in the colon; E2F8 in the rectum and DMD and hsa-miR-130b-3p in the rectosigmoid junction. We also identified different biological pathways that highlight differences in CRC behavior at different anatomical sites, thus reinforcing the importance of correctly identifying the tumor site.

Conclusions

Several potential prognostic markers for colon, rectum, and rectosigmoid junction cancer were found. CeRNA networks could provide better understanding of the differences between, and common factors in, prognosis of colon, rectum, and rectosigmoid junction cancer.
Tumor-Associated Macrophages: A Potential Target for Cancer Therapy
Authors: Tan Yifan , Wang Min , Zhang Yang , Ge Shengyang , Zhong Fan , Xia Guowei , Sun Chuanyu
Published: June 2021

Macrophages, an important class of innate immune cells that maintain body homeostasis and ward off foreign pathogens, exhibit a high degree of plasticity and play a supportive role in different tissues and organs. Thus, dysfunction of macrophages may contribute to advancement of several diseases, including cancer. Macrophages within the tumor microenvironment are known as tumor-associated macrophages (TAMs), which typically promote cancer cell initiation and proliferation, accelerate angiogenesis, and tame anti-tumor immunity to promote tumor progression and metastasis. Massive infiltration of TAMs or enrichment of TAM-related markers usually indicates cancer progression and a poor prognosis, and consequently tumor immunotherapies targeting TAMs have gained significant attention. Here, we review the interaction between TAMs and cancer cells, discuss the origin, differentiation and phenotype of TAMs, and highlight the role of TAMs in pro-cancer functions such as tumor initiation and development, invasive metastasis, and immunosuppression. Finally, we review therapies targeting TAMs, which are very promising therapeutic strategies for malignant tumors.
Healing in a Social Context: The Importance of Clinician and Patient Relationship
Authors: Wampold Bruce E.
Published: June 2021

When a patient presents to a health provider, the course of the disorder is composed of three effects: natural effects, specific effects, and contextual effects. Part of the contextual effect is due to the relationship between the healer and the patient. Social healing appears to be present in eusocial species and particularly well-developed in humans. Evidence for the importance of the relationship in healing is found in placebo studies, including placebo analgesics, medicine, and psychotherapy. Although the theory for how the relationship is therapeutic is not well-developed, four possible mechanisms are discussed. The implications for health care and the treatment of pain are discussed.
Clinical Analysis of Pediatric Systemic Juvenile Xanthogranulomas: A Retrospective Single-Center Study
Authors: Lian Hongyun , Wei Ang , He Lejian , Yang Ying , Ma Honghao , Zhang Liping , Guan Yitong , Zhang Qing , Wang Dong , Li Zhigang , Zhang Rui , Wang Tianyou
Published: June 2021

Objective: To investigate the clinical characteristics, treatment, and prognosis of children with systemic juvenile xanthogranuloma (JXG).

Methods: Clinical data of children with JXG who were hospitalized in Beijing Children's Hospital, Capital Medical University, from January 2012 to December 2019 were retrospectively analyzed, including clinical manifestations, laboratory determinations, treatment, and prognosis of the children. Patients were treated with vindesine + prednisone as the first-line treatment and cytarabine + vindesine + dexamethasone ± cladribine as the second-line treatment.

Results: Ten patients, including 8 males and 2 females, with a median of onset age of 1.95 (0.80–7.30) years, exhibited multi-system dysfunction. The median age of diagnosis was 2.45 (1.30–12.10) years. The most common location of extracutaneous lesions was the central nervous system (6 cases), followed by the lung (5 cases) and bone (4 cases). Nine patients underwent first-line chemotherapy, and 6 patients underwent second-line chemotherapy, including 5 patients with poorly controlled disease after first-line treatment. The median observation time was 29 (3–115) months. Nine patients survived, whereas one patient died of respiratory failure caused by pulmonary infection. At the end of follow-up, 7 patients were in active disease (AD)/regression state (AD-better), and 2 patients were in an AD/stable state (AD-stable). Three patients had permanent sequelae, mainly central diabetes insipidus. The rates of response to the first-line treatment and the second-line treatment were 40.0 and 66.7% respectively.

Conclusion: The chemotherapy protocol for Langerhans cell histiocytosis (LCH) may be effective for patients with systemic JXG. Central nervous system involvement may not impact overall survival, but serious permanent sequelae may occur.
Angelicin Alleviates Post-Trauma Osteoarthritis Progression by Regulating Macrophage Polarization via STAT3 Signaling Pathway
Authors: Tian Zhansong , Zeng Fanchun , Zhao Chunrong , Dong Shiwu
Published: June 2021

Post-trauma osteoarthritis (PTOA) is the most common articular disease characterized by degeneration and destruction of articular cartilage (Bultink and Lems, Curr. Rheumatol Rep., 2013, 15, 328). Inflammatory response of local joint tissue induced by trauma is the most critical factor accelerating osteoarthritis (OA) progression (Sharma et al., 2019; Osteoarthritis. Cartilage, 28, 658–668). M1/M2 macrophages polarization and repolarization participates in local inflammation, which plays a major role in the progression of OA (Zhang et al., 2018; Ann. Rheum. Dis., 77, 1524–1534). The regulating effect of macrophage polarization has been reported as a potential therapy to alleviate OA progression. Synovitis induced by polarized macrophages could profoundly affect the chondrocyte and cartilage matrix (Zhang et al., 2018; Ann. Rheum. Dis., 77, 1524–1534). Generally, anti-inflammatory medications widely used in clinical practice have serious side effects. Therefore, we focus on exploring a new therapeutic strategy with fewer side effects to alleviate the synovitis. Angelicin (ANG) is traditional medicine used in various folk medicine. Previous studies have revealed that angelicin has an inhibitory effect on inflammation (Wei et al., 2016; Inflammation, 39, 1876–1882), tumor growth (Li et al., 2016; Oncology reports, 36, 3,504–3,512; Wang et al., 2017; Molecular Medicine Reports, 16, 5441–5449), DNA damage (Li et al., 2019; Exp. Ther. Med., 18, 1899–1906), and virus proliferation (Li et al., 2018; Front. Cell. Infect. Microbiol., 8, 178). But its specific effects on influencing the process of OA were rarely reported. In this study, the molecular mechanism of angelicin in vivo and in vitro was clearly investigated. Results showed that angelicin could regulate the M1/M2 ratio and function and alleviate the development of PTOA in the meanwhile. Bone marrow monocytes were isolated and induced by macrophage colony-stimulating factor (M-CSF), lipopolysaccharide (LPS) and interferon (IFN)-γ for M1 polarization and interleukin (IL)-4/IL-13 for M2 polarization. Subsequently, repolarization intervention was performed. The results indicate that angelicin can repolarize M1 toward M2 macrophages by upregulating the expression of CD9. Besides, angelicin can also protect and maintain M2 polarization in the presence of LPS/IFN-γ, and subsequently downregulate the expression of inflammatory mediators such as IL-1β and TNF-α. Mechanistically, angelicin can activate the p-STAT3/STAT3 pathway by conducting CD9/gp130 to repolarize toward M2 macrophages. These results suggest angelicin can alleviate the progression of OA by regulating M1/M2 polarization via the STAT3/p-STAT3 pathway. Therefore, angelicin may have a promising application and potential therapeutic value in OA clinical treatment.
Algebraic Structure and Poisson Integral Method of Snake-Like Robot Systems
Authors: Jing-Li Fu , Chun Xiang , Lei Meng
Published: June 2021

The algebraic structure and Poisson's integral of snake-like robot systems are studied. The generalized momentum, Hamiltonian function, generalized Hamilton canonical equations, and their contravariant algebraic forms are obtained for snake-like robot systems. The Lie-admissible algebra structures of the snake-like robot systems are proved and partial Poisson integral methods are applied to the snake-like robot systems. The first integral methods of the snake-like robot systems are given. An example is given to illustrate the results.
From Brain Organoids to Networking Assembloids: Implications for Neuroendocrinology and Stress Medicine
Authors: Makrygianni Evanthia A. , Chrousos George P.
Published: June 2021

Brain organoids are three-dimensional cultures that contain multiple types of cells and cytoarchitectures, and resemble fetal human brain structurally and functionally. These organoids are being used increasingly to model brain development and disorders, however, they only partially recapitulate such processes, because of several limitations, including inability to mimic the distinct cortical layers, lack of functional neuronal circuitry as well as non-neural cells and gyrification, and increased cellular stress. Efforts to create improved brain organoid culture systems have led to region-specific organoids, vascularized organoids, glia-containing organoids, assembloids, sliced organoids and polarized organoids. Assembloids are fused region-specific organoids, which attempt to recapitulate inter-regional and inter-cellular interactions as well as neural circuitry development by combining multiple brain regions and/or cell lineages. As a result, assembloids can be used to model subtle functional aberrations that reflect complex neurodevelopmental, neuropsychiatric and neurodegenerative disorders. Mammalian organisms possess a highly complex neuroendocrine system, the stress system, whose main task is the preservation of systemic homeostasis, when the latter is threatened by adverse forces, the stressors. The main central parts of the stress system are the paraventricular nucleus of the hypothalamus and the locus caeruleus/norepinephrine-autonomic nervous system nuclei in the brainstem; these centers innervate each other and interact reciprocally as well as with various other CNS structures. Chronic dysregulation of the stress system has been implicated in major pathologies, the so-called chronic non-communicable diseases, including neuropsychiatric, neurodegenerative, cardiometabolic and autoimmune disorders, which lead to significant population morbidity and mortality. We speculate that brain organoids and/or assembloids could be used to model the development, regulation and dysregulation of the stress system and to better understand stress-related disorders. Novel brain organoid technologies, combined with high-throughput single-cell omics and gene editing, could, thus, have major implications for precision medicine.
A Comparison of an Alternative Weight-Grading Model Against Chronological Age Group Model for the Grouping of Schoolboy Male Rugby Players
Authors: Lentin Grégory , Cumming Sean , Piscione Julien , Pezery Patrick , Bouchouicha Moez , Gadea José , Raymond Jean-Jacques , Duché Pascale , Gavarry Olivier
Published: June 2021

Objectives

Concerns regarding marked differences in the weights and body composition of young rugby players competing within the same age groups have led to the suggestion of alternative models for grouping young players. The aims of this study were (1) to compare variance in the body size and body composition of schoolboy rugby players (9 to 14 years), across weight- and age-grading models, and (2) to identify morphotypes for the weight model using Hattori’s body composition chart.

Materials and Methods

Skinfold thickness measurements were used to assess body fat mass (BF), fat-free mass (FFM), body fat mass index (BFMI), and fat-free mass index (FFMI). Standardized measure of height and weight were taken for all participants. Data were grouped according to the age categories of the French Rugby Federation (U11: Under 11 years, U13: Under 13 years, and U15: Under 15 years), and to the weight categories (W30–44.9; W45–59.9; and W60–79.9) carried out from 25th and 75th weight percentile in each age category. Body mass index status (NW normal-weight versus OW/OB overweight/obese) was considered. Extreme morphotypes are characterized from BFMI and FFMI in the weight-grading model on Hattori’s body composition chart.

Results

The dispersion of anthropometric characteristics decreased significantly for the weight model, except for height in all groups and BFMI for U13. Among NW, 3, 1.8, and 0% upgraded; 18.2, 68.7, and 45.5% downgraded; among OW, 50, 21.5, and 12.5%; and among OB, 91.3, 83.3, and 74.6% upgraded, respectively, in U11, U13, U15. FFMI/BFMI were correlated in U11 (r = 0.80, p < 0.001), U13 (r = 0.66, p < 0.001), and U15 (r = 0.77, p < 0.001). There was no significant correlation in W45–59.9 and low correlations in W30–44.9 (r = 0.25, p < 0.001) and W60–79.9 (r = 0.29, p < 0.001). Significant grading difference between the centroids (p < 0.05) and the distribution deviates from centroids of BFMI and FFMI (p < 0.0001) were noted between the two models. Thirteen players were located in adipo-slender, twenty-three in adipo-solid, twenty-two in lean-slender, and two located in the lean-solid morphotype in weight model.

Conclusion

A weight-grading model should be considered to limit mismatches in anthropometric variables. However, variations of body composition also persisted for this model. Hattori’s body composition chart allowed more detailed examination of morphological atypicalities among schoolboy rugby players.
Correlation Among Phenotypic Parameters Related to the Growth and Photosynthesis of Strawberry (Fragaria × ananassa Duch.) Grown Under Various Light Intensity Conditions
Authors: Choi Hyo Gil
Published: June 2021

The objective of this study was to investigate characteristics of phenotypic parameters such as physiology, yield, and fruit quality responses of strawberry (Fragaria × ananassa Duch.) to various light intensity conditions (VLICs), and to determine the correlations among these phenotypic parameters. Strawberry plants were cultivated in a smart greenhouse separated into four areas, three of which were completely shaded by curtains from 20:00 until 10:00 (3 hS), 12:00 (5 hS), and 14:00 (7 hS), respectively. The fourth area was a non-shaded control treatment (0 hS). The ambient light intensities during the experimental period for the 0, 3, 5, and 7 hS treatments were 1,285, 1,139, 770, and 364 mol⋅m–2, respectively. Strawberry plants grown under low light intensity conditions experienced decreases in photosynthetic rate, stomatal conductance, and sugar accumulation compared to the 0 hS. Petiole generation and fruit yield were also sharply decreased in proportion to the degree of decrease in light intensity. In contrast, photosynthetic pigment content was shown to increase under low light conditions. Organic acid contents (excluding acetic acid) and leaflet size did not change significantly under low light conditions compared to the 0 hS. Changes to light intensity are considered to induce changes to the phenotypic characteristics of strawberry plants to favor growth using the energy and carbon skeletons obtained through respiration and photosynthesis. In the 7 hS treatment, where light intensity was drastically reduced, NPQ, qP, and RFd values as chlorophyll a fluorescence parameters were significantly lowered, which could indicate their measurement as an important technique to check the stress response of plants grown in low light conditions.
Plant-Mediated Rhizosphere Oxygenation in the Native Invasive Salt Marsh Grass Elymus athericus
Authors: Koop-Jakobsen Ketil , Meier Robert J. , Mueller Peter
Published: June 2021

In the last decades, the spread of Elymus athericus has caused significant changes to the plant community composition and ecosystem services of European marshes. The distribution of E. athericus was typically limited by soil conditions characteristic for high marshes, such as low flooding frequency and high soil aeration. However, recently the spread of E. athericus has begun to also include low-marsh environments. A high-marsh ecotype and a low-marsh ecotype of E. athericus have been described, where the latter possess habitat-specific phenotypic traits facilitating a better adaption for inhabiting low-marsh areas. In this study, planar optodes were applied to investigate plant-mediated sediment oxygenation in E. athericus, which is a characteristic trait for marsh plants inhabiting frequently flooded environments. Under waterlogged conditions, oxygen (O2) was translocated from aboveground sources to the roots, where it leaked out into the surrounding sediment generating oxic root zones below the sediment surface. Oxic root zones were clearly visible in the optode images, and no differences were found in the O2-leaking capacity between ecotypes. Concentration profiles measured perpendicular to the roots revealed that the radius of the oxic root zones ranged from 0.5 to 2.6 mm measured from the root surface to the bulk anoxic sediment. The variation of oxic root zones was monitored over three consecutive light–dark cycles (12 h/12 h). The O2 concentration of the oxic root zones was markedly reduced in darkness, yet the sediment still remained oxic in the immediate vicinity of the roots. Increased stomatal conductance improving the access to atmospheric O2 as well as photosynthetic O2 production are likely factors facilitating the improved rhizosphere oxygenation during light exposure of the aboveground biomass. E. athericus’ capacity to oxygenate its rhizosphere is an inheritable trait that may facilitate its spread into low-marsh areas. Furthermore, this trait makes E. athericus a highly competitive species in marshes facing the effects of accelerated sea-level rise, where waterlogged sediment conditions could become increasingly pronounced.
Validating the Strategic Deployment of Blackleg Resistance Gene Groups in Commercial Canola Fields on the Canadian Prairies
Authors: Cornelsen Justine , Zou Zhongwei , Huang Shuanglong , Parks Paula , Lange Ralph , Peng Gary , Fernando W. G. Dilantha
Published: June 2021

Blackleg, caused by the fungal pathogen Leptosphaeria maculans, is a serious threat to canola (Brassica napus L.) production in western Canada. Crop scouting and extended crop rotation, along with the use of effective genetic resistance, have been key management practices available to mitigate the impact of the disease. In recent years, new pathogen races have reduced the effectiveness of some of the resistant cultivars deployed. Strategic deployment and rotation of major resistance (R) genes in cultivars have been used in France and Australia to help increase the longevity of blackleg resistance. Canada also introduced a grouping system in 2017 to identify blackleg R genes in canola cultivars. The main objective of this study was to examine and validate the concept of R gene deployment through monitoring the avirulence (Avr) profile of L. maculans population and disease levels in commercial canola fields within the Canadian prairies. Blackleg disease incidence and severity was collected from 146 cultivars from 53 sites across Manitoba, Saskatchewan, and Alberta in 2018 and 2019, and the results varied significantly between gene groups, which is likely influenced by the pathogen population. Isolates collected from spring and fall stubble residues were examined for the presence of Avr alleles AvrLm1, AvrLm2, AvrLm3, AvrLm4, AvrLm5, AvrLm6, AvrLm7, AvrLm9, AvrLm10, AvrLm11, AvrLepR1, AvrLepR2, AvrLep3, and AvrLmS using a set of differential host genotypes carrying known resistance genes or PCR-based markers. The Simpson’s evenness index was very low, due to two dominant L. maculans races (AvrLm2-4-5-6-7-10-11 and AvrLm2-5-6-7-10-11) representing 49% of the population, but diversity of the population was high from the 35 L. maculans races isolated in Manitoba. AvrLm6 and AvrLm11 were found in all 254 L. maculans isolates collected in Manitoba. Knowledge of the blackleg disease levels in relation to the R genes deployed, along with the L. maculans Avr profile, helps to measure the effectiveness of genetic resistance.
Regulation and Physiological Significance of the Nuclear Shape in Plants
Authors: Goto Chieko , Hara-Nishimura Ikuko , Tamura Kentaro
Published: June 2021

The shape of plant nuclei varies among different species, tissues, and cell types. In Arabidopsis thaliana seedlings, nuclei in meristems and guard cells are nearly spherical, whereas those of epidermal cells in differentiated tissues are elongated spindle-shaped. The vegetative nuclei in pollen grains are irregularly shaped in angiosperms. In the past few decades, it has been revealed that several nuclear envelope (NE) proteins play the main role in the regulation of the nuclear shape in plants. Some plant NE proteins that regulate nuclear shape are also involved in nuclear or cellular functions, such as nuclear migration, maintenance of chromatin structure, gene expression, calcium and reactive oxygen species signaling, plant growth, reproduction, and plant immunity. The shape of the nucleus has been assessed both by labeling internal components (for instance chromatin) and by labeling membranes, including the NE or endoplasmic reticulum in interphase cells and viral-infected cells of plants. Changes in NE are correlated with the formation of invaginations of the NE, collectively called the nucleoplasmic reticulum. In this review, what is known and what is unknown about nuclear shape determination are presented, and the physiological significance of the control of the nuclear shape in plants is discussed.
Psychological Complications at 3 Months Following Stroke: Prevalence and Correlates Among Stroke Survivors in Lebanon
Authors: Khazaal Walaa , Taliani Maram , Boutros Celina , Abou-Abbas Linda , Hosseini Hassan , Salameh Pascale , Sadier Najwane Said
Published: June 2021

Introduction: Stroke continues to be a common and debilitating medical condition which has a significant effect on public health as the second primary source of mortality and the third major root of disability worldwide. A wide range of complications affecting the survivor's life and interfering with the recovery process usually follows stroke; anxiety and depression are considered one of the major complications post-stroke. This study sought to investigate the short-term psychological consequences of stroke among Lebanese survivors and to identify their correlates.

Methods: This study is a prospective observational epidemiological study. 143 stroke patients admitted to hospitals in Mount Lebanon and Beirut between February and May 2018.were included in this study. Assessments of complications were carried out at 3 months post-stroke by completing a 30-min face-to-face interview questionnaire. The survey included the socio-demographic -characteristics of the patients, their lifestyle, health indicators, the severity of stroke, and the post-stroke consequences disturbing their quality of life.

Results: Complications were recorded for 117 stroke survivors (mean age, 72.46 years; 60.7% male). The analysis of results 3 months post stroke showed that 29 survivors suffered from neuropathic pain (24.8%), 110 (94%) suffered from fatigue, and 81 (69.2%) from cognitive impairment. High rates of anxiety (51.3%), and depression (76.1%) were recorded as well. Multivariate logistic regression confirmed that there is a significant association between depression and the following variables: anxiety (OR = 4.814, p-value = 0.017), pain (OR = 6.868, p-value = 0.002), and physical activity, which acts as a protective factor against depression (OR = 0.261; p-value = 0.029). However, the results of the multivariate logistic regression analysis for anxiety indicated that immobility-related complications increase the risk of anxiety by 8.457 in sedentary duration longer than 12 h (ORa = 8.457, p-value = 0.01). Furthermore, patients with neuropathic pain (24.8%) are 3.858 times more likely to have anxiety compared to patients without neuropathic pain (ORa = 3.858, p-value = 0.019).

Conclusion: Using a patient-centered structure more interventions should take place to evaluate stroke survivors' outcomes, and organize rehabilitation services that deal with stroke consequences, particularly high anxiety and depression levels, which are prevalent and persistent among the Lebanese stroke survivors.
Etiology of Burst Suppression EEG Patterns
Authors: Shanker Akshay , Abel John H. , Schamberg Gabriel , Brown Emery N.
Published: June 2021

Burst-suppression electroencephalography (EEG) patterns of electrical activity, characterized by intermittent high-power broad-spectrum oscillations alternating with isoelectricity, have long been observed in the human brain during general anesthesia, hypothermia, coma and early infantile encephalopathy. Recently, commonalities between conditions associated with burst-suppression patterns have led to new insights into the origin of burst-suppression EEG patterns, their effects on the brain, and their use as a therapeutic tool for protection against deleterious neural states. These insights have been further supported by advances in mechanistic modeling of burst suppression. In this Perspective, we review the origins of burst-suppression patterns and use recent insights to weigh evidence in the controversy regarding the extent to which burst-suppression patterns observed during profound anesthetic-induced brain inactivation are associated with adverse clinical outcomes. Whether the clinical intent is to avoid or maintain the brain in a state producing burst-suppression patterns, monitoring and controlling neural activity presents a technical challenge. We discuss recent advances that enable monitoring and control of burst suppression.
Cross-National Outcomes of a Digital Weight Loss Intervention in the United States, Canada, United Kingdom and Ireland, and Australia and New Zealand: A Retrospective Analysis
Authors: Yang Qiuchen , Mitchell Ellen Siobhan , Ho Annabell S. , DeLuca Laura , Behr Heather , Michaelides Andreas
Published: June 2021

Mobile health (mHealth) interventions are ubiquitous and effective treatment options for obesity. There is a widespread assumption that the mHealth interventions will be equally effective in other locations. In an initial test of this assumption, this retrospective study assesses weight loss and engagement with an mHealth behavior change weight loss intervention developed in the United States (US) in four English-speaking regions: the US, Australia and New Zealand (AU/NZ), Canada (CA), and the United Kingdom and Ireland (UK/IE). Data for 18,459 participants were extracted from the database of Noom's Healthy Weight Program. Self-reported weight was collected every week until program end (week 16). Engagement was measured using user-logged and automatically recorded actions. Linear mixed models were used to evaluate change in weight over time, and ANOVAs evaluated differences in engagement. In all regions, 27.2–33.2% of participants achieved at least 5% weight loss by week 16, with an average of 3–3.7% weight loss. Linear mixed models revealed similar weight outcomes in each region compared to the US, with a few differences. Engagement, however, significantly differed across regions (P < 0.001 on 5 of 6 factors). Depending on the level of engagement, the rate of weight loss over time differed for AU/NZ and UK/IE compared to the US. Our findings have important implications for the use and understanding of digital weight loss interventions worldwide. Future research should investigate the determinants of cross-country engagement differences and their long-term effects on intervention outcomes.
Development of a Digital Video-Based Occupational Risk Assessment Method
Authors: Beese Nils Ove , Rodriguez Francisca S. , Spilski Jan , Lachmann Thomas
Published: June 2021

The development and implementation of an observational video-based risk assessment is described. Occupational risk assessment is one of the most important yet also challenging tasks for employers. Most assessment tools to date use questionnaires, expert interviews, and similar tools. Video analysis is a promising tool for risk assessment, but it needs an objective basis. A video of a plastering worker was recorded using a 360° camera. The recording was then analyzed using the developed observational matrix concerning Work Characteristics, Work Activities as well as potential risks. Risk factors present during the video of the work included lifting, fall from ladder, hazardous substances as well as occasionally bad posture. The worker had no or just one risk factor present during most of the time of the video recording, while only 16 s with more than one risk factor present according to the observational matrix. The paper presents a promising practical method to assess occupational risks on a case-by-case basis. It can help with the risk assessment process in companies which is required by law in some industrialized countries. The matrix in combination with video analysis is a first step toward digital observational risk assessment. It can also be the basis of an automated risk assessment process.
Vacuum-Ultraviolet Absorption Spectra of Icy C2H4 at 13–60 K
Authors: Lo Jen-Iu , Ghosh Rana , Lu Hsiao-Chi , Hung Wei-Hsiu , Cheng Bing-Ming
Published: June 2021

The thermal variation of absorption spectra of icy ethene in wavelength range 105–220 nm was measured from 13 to 100 K using a synchrotron as light source. Sublimation of icy ethene began above 62 K, resulting in decreasing absorption. The absorption of icy ethene increased at wavelengths less than about 150 nm with increasing temperature from 13 to 60 K, but decreased beyond above 150 nm. According to detailed examination, the absorption spectra of icy ethene intersected at isosbestic point 147.0 nm from 13 to 17 K, whereas those varied absorption profiles crossed at another point, 150.6 nm, from 23 to 60 K. These results indicate that ethene ices might exhibit three structures within temperature range 13–60 K. This work enhances our understanding of the spectra of icy ethene at low temperatures and our knowledge of its astrochemistry and astrophysics in cold astro-environments.
A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1
Authors: Zarski Lila M. , Vaala Wendy E. , Barnett D. Craig , Bain Fairfield T. , Soboll Hussey Gisela
Published: June 2021

Equine herpesvirus 1 (EHV-1) ubiquitously infects horses worldwide and causes respiratory disease, abortion, and equine herpesvirus myeloencephalopathy. Protection against EHV-1 disease is elusive due to establishment of latency and immune-modulatory features of the virus. These include the modulation of interferons, cytokines, chemokines, antigen presentation, and cellular immunity. Because the modulation of immunity likely occurs at the site of first infection—the respiratory epithelium, we hypothesized that the mucosal influenza vaccine Flu Avert® I.N. (Flu Avert), which is known to stimulate strong antiviral responses, will enhance antiviral innate immunity, and that these responses would also provide protection from EHV-1 infection. To test our hypothesis, primary equine respiratory epithelial cells (ERECs) were treated with Flu Avert, and innate immunity was evaluated for 10 days following treatment. The timing of Flu Avert treatment was also evaluated for optimal effectiveness to reduce EHV-1 replication by modulating early immune responses to EHV-1. The induction of interferons, cytokine and chemokine mRNA expression, and protein secretion was evaluated by high-throughput qPCR and multiplex protein analysis. Intracellular and extracellular EHV-1 titers were determined by qPCR. Flu Avert treatment resulted in the modulation of IL-8, CCL2, and CXCL9 starting at days 5 and 6 post-treatment. Coinciding with the timing of optimal chemokine induction, our data also suggested the same timing for reduction of EHV-1 replication. In combination, our results suggest that Flu Avert may be effective at counteracting some of the immune-modulatory properties of EHV-1 at the airway epithelium and the peak for this response occurs 5–8 days post-Flu Avert treatment. Future in vivo studies are needed to investigate Flu Avert as a prophylactic in situations where EHV-1 exposure may occur.
Advancing Motivation Feedforward Control of Permanent Magnetic Linear Oscillating Synchronous Motor for High Tracking Precision
Authors: Jiao Zongxia , Cao Yuan , Yan Liang , Li Xinglu , Zhang Lu , Li Yang
Published: June 2021

Linear motors have promising application to industrial manufacture because of their direct motion and thrust output. A permanent magnetic linear oscillating synchronous motor (PMLOSM) provides reciprocating motion which can drive a piston pump directly having advantages of high frequency, high reliability, and easy commercial manufacture. Hence, researching the tracking performance of PMLOSM is of great importance to realizing its popularization and application. Traditional PI control cannot fulfill the requirement of high tracking precision, and PMLOSM performance has high phase lag because of high control stiffness. In this paper, an advancing motivation feedforward control (AMFC), which is a combination of advancing motivation signal and PI control signal, is proposed to obtain high tracking precision of PMLOSM. The PMLOSM inserted with AMFC can provide accurate trajectory tracking at a high frequency. Compared with single PI control, AMFC can reduce the phase lag from −18 to −2.7 degrees, which shows great promotion of the tracking precision of PMLOSM. In addition, AMFC will promote the application of PMLOSM to other working conditions needing high frequency reciprocating tracking performance and give PMLOSM greater future prospects.
Theory of Contrail Formation for Fuel Cells
Authors: Gierens Klaus
Published: June 2021

The theory of contrail formation for fuel cells is derived. It is a variant of the well-known Schmidt-Appleman theory. The contrail factor or G-factor for fuel cells is much larger than for jet engines, such that condensation of the exhaust water vapour can happen even at the Earth’s surface in sufficiently cold (a few degrees above zero) weather. Contrail formation from fuel cells will occur frequently in the lower troposphere and is unavoidable below moderate temperature limits, in the upper troposphere and in the stratosphere. Despite the high frequency of contrail formation from fuel cells, their climate impact is lower than that of contrails from jet engines. Most fuel cell contrails will be short and those persistent ones will be optically thinner and have on average a shorter lifetime than traditional persistent contrails. From a climate point of view, the introduction of fuel cells into aviation can be recommended.
Genetic Progress Achieved during 10 Years of Selective Breeding for Honeybee Traits of Interest to the Beekeeping Industry
Authors: Maucourt Ségolène , Fortin Frédéric , Robert Claude , Giovenazzo Pierre
Published: June 2021

Genetic improvement programs have resulted in spectacular productivity gains for most animal species in recent years. The introduction of quantitative genetics and the use of statistical models have played a fundamental role in achieving these advances. For the honeybee (Apis mellifera), genetic improvement programs are still rare worldwide. Indeed, genetic and reproductive characteristics are more complex in honeybees than in other animal species, which presents additional challenges for access genetic selection. In recent years, advances in informatics have allowed statistical modelling of the honeybee, notably with the BLUP-animal model, and access to genetic selection for this species is possible now. The aim of this project was to present the genetic progress of several traits of interest to the Canadian beekeeping industry (hygienic behavior, honey production and spring development) achieved in our selection program since 2010. Our results show an improvement of 0.30% per year for hygienic behavior, 0.63 kg per year for honey production and 164 brood cells per year for spring development. These advances have opened a new era for our breeding program and sharing this superior genetic available to beekeepers will contribute to the sustainability and self-sufficiency of the beekeeping industry in Canada.
The Usefulness of Mixtures with Festulolium braunii for the Regeneration of Grassland under Progressive Climate Change
Authors: Kitczak Teodor , Jänicke Heidi , Bury Marek , Malinowski Ryszard
Published: June 2021

The climate warming and changes in weather patterns in Europe are negatively affecting the structure of grassland swards. The disappearance of valuable forage grasses can be observed. Therefore, in order to keep grassland productivity high, high-yielding grasses of good quality that are resistant to changes in the weather are being sought. At the moment there are few publications which present the long-term influence of changing weather conditions on meadow sward structure and its fodder value. This study aimed to assess the suitability of grass mixtures with Festulolium braunii for grassland renewal by full tillage on organic soil, taking into account atmospheric conditions. The experiments were carried out in the years 2009–2018 on a meadow complex located on organic soil in the Randow river valley, near Ramin (Germany). Grassland restoration was carried out by the method of full cultivation in three experiments with different proportions of Festulolium braunii and Lolium perenne (the existing grassland was eliminated and seeds of a new grass mix were sown). The meadow experiments were established using the method of random blocks in four repetitions Detailed studies included: floristic composition of meadow sward, fresh and dry mass yields, and content of: crude protein, soluble sugars, raw fiber, and net energy NEL (net energy lactation) concentration. Festulolium braunii, Poa pratensis, Dactylis glomerata, and Phleum pratense proved to be more resistant to low temperatures in winter and excess water in spring than Lolium perenne. The largest production potential of fresh and dry mass was found in mixtures with a high content of Festulolium braunii Festulolium braunii tolerates varied and unfavorable weather conditions (frosts during snowless winters, spring frosts and waterlogging, and droughts) very well and provides a stable good quality sward yield (favourably affected the carbohydrate and protein content of the sward). Lolium perenne, on the other hand, decreases its share in the sward under unfavorable weather conditions.
Influence of Fertilization and Mycorrhizae on the Nutritional Status of Rhododendron (Rhododendron hybridum) in a Nursery
Authors: Jarosz Zbigniew , Michałojć Zenia , Pitura Karolina , Dzida Katarzyna , Koter Michał
Published: June 2021

Background: This study of the large-flowered azalea cv. ‘Anneke’ investigated the impact of two factors, i.e., methods of fertilization and mycorrhization, on the nutritional status of plants during three years of nursery cultivation. Methods: Single mineral fertilizers, a slow-release fertilizer Hortiform pH (SRF), and fertigation in combination with mycorrhization of plants, were applied. Plant roots were inoculated with fungi from the genera Oidiodendron and Hymenoscyphus sp. The nutritional status of the large-flowered azalea in the first three years of cultivation was assessed based on macroelements. Results: The analyses revealed significantly higher content of nitrogen, phosphorus and calcium in the leaves of plants inoculated with fungal mycelium. A beneficial effect of plant mycorrhization on plant nutritional status, i.e., higher levels of nitrogen, phosphorus, potassium, calcium, and magnesium, was noted in the second and third years of azalea cultivation. Conclusions: Significant amounts of the nutrients were utilized in the middle of the growing season and almost fully utilized after the season. Hence, the necessity to supplement nutrients in each subsequent year of plant vegetation was postulated. Fertigation was shown to require further improvement of the nutrient solution.
An Ethical and Societal Analysis for Biotechnological Methods in Plant Breeding
Authors: Louwaars Niels , Jochemsen Henk
Published: June 2021

Technological developments in plant breeding, notably cisgenesis and gene editing, require a rethinking of biotechnology policies. In addition to legal debates about the definition of genetic modification in the Cartagena Protocol and at national and supra-national levels, and debates about the safety of the resulting products for mankind and environment, discussions are ongoing in society concerning ethical and societal questions. In this paper, we analyse the main ethical issues that need to be taken into account when evaluating contemporary plant breeding techniques. After a brief description of the state of the art in plant breeding, we discuss these main ethical issues. We take Consequentialist, Deontological and Virtue ethics as bases of our analysis. This results in a generally positive approach to gene editing, but also highlights several concerns, predominantly used by particular groups in society. This leads to a moral incentive toward transparency and options for operationalizing consumer choice.