bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly
Authors: Bennison Daniel J. , Irving Sophie E. , Corrigan Rebecca M.
Published: October 2019

Many facets of ribosome biogenesis and function, including ribosomal RNA (rRNA) transcription, 70S assembly and protein translation, are negatively impacted upon induction of a nutrient stress-sensing signalling pathway termed the stringent response. This stress response is mediated by the alarmones guanosine tetra- and penta-phosphate ((p)ppGpp), the accumulation of which leads to a massive cellular response that slows growth and aids survival. The 70S bacterial ribosome is an intricate structure, with assembly both complex and highly modular. Presiding over the assembly process is a group of P-loop GTPases within the TRAFAC (Translation Factor Association) superclass that are crucial for correct positioning of both early and late stage ribosomal proteins (r-proteins) onto the rRNA. Often described as ‘molecular switches’, members of this GTPase superfamily readily bind and hydrolyse GTP to GDP in a cyclic manner that alters the propensity of the GTPase to carry out a function. TRAFAC GTPases are considered to act as checkpoints to ribosome assembly, involved in binding to immature sections in the GTP-bound state, preventing further r-protein association until maturation is complete. Here we review our current understanding of the impact of the stringent response and (p)ppGpp production on ribosome maturation in prokaryotic cells, focusing on the inhibition of (p)ppGpp on GTPase-mediated subunit assembly, but also touching upon the inhibition of rRNA transcription and protein translation.
Regulation of Mitochondria-Associated Membranes (MAMs) by NO/sGC/PKG Participates in the Control of Hepatic Insulin Response
Authors: Bassot Arthur , Chauvin Marie-Agnès , Bendridi Nadia , Ji-Cao Jingwei , Vial Guillaume , Monnier Léa , Bartosch Birke , Alves Anaïs , Cottet-Rousselle Cécile , Gouriou Yves , Rieusset Jennifer , Morio Béatrice
Published: October 2019

Under physiological conditions, nitric oxide (NO) produced by the endothelial NO synthase (eNOS) upregulates hepatic insulin sensitivity. Recently, contact sites between the endoplasmic reticulum and mitochondria named mitochondria-associated membranes (MAMs) emerged as a crucial hub for insulin signaling in the liver. As mitochondria are targets of NO, we explored whether NO regulates hepatic insulin sensitivity by targeting MAMs. In Huh7 cells, primary rat hepatocytes and mouse livers, enhancing NO concentration increased MAMs, whereas inhibiting eNOS decreased them. In vitro, those effects were prevented by inhibiting protein kinase G (PKG) and mimicked by activating soluble guanylate cyclase (sGC) and PKG. In agreement with the regulation of MAMs, increasing NO concentration improved insulin signaling, both in vitro and in vivo, while eNOS inhibition disrupted this response. Finally, inhibition of insulin signaling by wortmannin did not affect the impact of NO on MAMs, while experimental MAM disruption, using either targeted silencing of cyclophilin D or the overexpression of the organelle spacer fetal and adult testis-expressed 1 (FATE-1), significantly blunted the effects of NO on both MAMs and insulin response. Therefore, under physiological conditions, NO participates to the regulation of MAM integrity through the sGC/PKG pathway and concomitantly improves hepatic insulin sensitivity. Altogether, our data suggest that the induction of MAMs participate in the impact of NO on hepatocyte insulin response.
Crack Initiation Criteria in EBC under Thermal Stress
Authors: Kawai Emi , Kakisawa Hideki , Kubo Atsushi , Yamaguchi Norio , Yokoi Taishi , Akatsu Takashi , Kitaoka Satoshi , Umeno Yoshitaka
Published: October 2019

For design of multi-layered environmental barrier coatings (EBCs), it is essential to assure mechanical reliability against interface crack initiation and propagation induced by thermal stress owing to a misfit of the coefficients of thermal expansion between the coating layers and SiC/SiC substrate. We conducted finite element method (FEM) analyses to evaluate energy release rate (ERR) for interface cracks and performed experiment to obtain interface fracture toughness to assess mechanical reliability of an EBC with a function of thermal barrier (T/EBC; SiC/SiAlON/mullite/Yb-silicate gradient composition layer/Yb2SiO5 with porous segment structure) on an SiC/SiC substrate under thermal stress due to cooling in fabrication process. Our FEM analysis revealed that a thinner SiAlON layer and a thicker mullite layer are most suitable to reduce ERRs for crack initiation at the SiC/SiAlON, SiAlON/mullite and mullite/Yb2Si2O7 interfaces. Interface fracture tests of the T/EBC with layer thicknesses within the proposed range exhibited fracture at the SiC/SiAlON and SiAlON/mullite interfaces. We also estimated the approximate fracture toughness for the SiC/SiAlON and SiAlON/mullite interfaces and lower limit of fracture toughness for the mullite/Yb2Si2O7 interface. Comparison between ERR and fracture toughness indicates that the fabricated T/EBC possesses sufficient mechanical reliability against interface crack initiation and propagation.
Low-Temperature Vapor-Solid Growth of ZnO Nanowhiskers for Electron Field Emission
Authors: Hedrich Carina , Haugg Stefanie , Pacarizi Leutrim , P. Furlan Kaline , Blick Robert H. , Zierold Robert
Published: October 2019

One-dimensional zinc oxide nanostructures have aroused interest from scientists and engineers for electron field emission applications because of their experimentally accessible high aspect ratio in combination with their low work function. A comprehensive study of the vapor-solid growth of zinc oxide (ZnO) nanowhiskers by utilizing zinc acetylacetonate hydrate and oxygen at low temperature (580 °C) is reported herein. The nanowhiskers morphology was investigated by varying different growth parameters, such as temperature, substrate type and position, gas flow, precursor amount, and growth time. According to the obtained parameter dependences, the process was optimized to achieve homogenous crystalline nanowhiskers with high aspect ratios and clearly defined surface facets and tips. We show electron field emission measurements from tailor-made ZnO nanowhiskers grown on n-doped silicon, titanium thin films, and free-standing silicon nitride membranes, revealing field emission turn-on fields significantly lower compared to a perfect flat ZnO thin film. Especially the latter devices—ZnO nanowhiskers on a free-standing membrane—might pave the way into a novel nanomembrane detector unit in proteomics, which can significantly extend the mass range of current time-of-flight mass spectrometers.
Hydrothermal Synthesis of (001) Facet Highly Exposed ZnO Plates: A New Insight into the Effect of Citrate
Authors: Luo Shirui , Chen Ruosong , Xiang Lan , Wang Jing
Published: October 2019

In this work, two synthesis routes were applied to investigate the effect of citrates on the construction of the ZnO structure. Well-dispersed ZnO plates with (001) facet highly exposed were prepared via one-step hydrothermal route, while ZnO nanoparticles were obtained via two-step route. In one-step route, citrates were added before the formation of Zn(OH)2 precursor, while citrates were added after the formation of Zn(OH)2. For the first time, the interaction between citrates and the Zn(OH)2 precursor was investigated and citrates that participated in the formation of Zn(OH)2 were the main cause for (001) facet exposed structure construction. A growth mechanism about the formation of ZnO plates in the presence of citrates was proposed. The as-prepared ZnO plates showed enhanced photocatalytic activity for the degradation of methylene blue (MB).
Structure and Electrical Properties of Na0.5Bi0.5TiO3 Epitaxial Films with (110) Orientation
Authors: Song Jianmin , Gao Jie , Zhang Suwei , Luo Laihui , Dai Xiuhong , Zhao Lei , Liu Baoting
Published: October 2019

Pt/Na0.5Bi0.5TiO3/La0.5Sr0.5CoO3 (Pt/NBT/LSCO) ferroelectric capacitors were fabricated on (110) SrTiO3 substrate. Both NBT and LSCO films were epitaxially grown on the (110) SrTiO3 substrate. It was found that the leakage current density of the Pt/NBT/LSCO capacitor is favorable to ohmic conduction behavior when the applied electric fields are lower than 60 kV/cm, and bulk-limited space charge-limited conduction takes place when the applied electric fields are higher than 60 kV/cm. The Pt/NBT/LSCO capacitor possesses good fatigue resistance and retention, as well as ferroelectric properties with Pr = 35 μC/cm2. The ferroelectric properties of the Pt/NBT/LSCO capacitor can be modulated by ultraviolet light. The effective polarization, ΔP, was reduced and the maximum polarization Pmax was increased for the Pt/NBT/LSCO capacitor when under ultraviolet light, which can be attributed to the increased leakage current density and non-reversible polarization P^ caused by the photo-generated carriers.
Resveratrol-Loaded Solid Lipid Nanoparticle Supplementation Ameliorates Physical Fatigue by Improving Mitochondrial Quality Control
Authors: Sun Jingyu , Zhou Yunhe , Su Yajuan , Li Sheng , Dong Jingmei , He Qing , Cao Yang , Lu Tianfeng , Qin Lili
Published: October 2019

Resveratrol (RSV) has various pharmacological effects; however, few studies have directly addressed the possible antifatigue effects of long-term endurance exercise. The clinical use of RSV is limited by its poor water solubility and extremely short plasma half-life. Solid lipid nanoparticles (SLNs) are considered as reasonable drug delivery systems to overcome some of these drawbacks and expand its applications. In this study, RSV-SLNs were successfully prepared through emulsification and low-temperature solidification. Results showed that RSV-SLN supplementation effectively enhanced endurance performance. RSV-SLN supplementation might enhance mitochondrial function by ameliorating mitochondrial quality control (QC), which was superior to RSV application. These results revealed an unexpected role of RSV-SLN compared with RSV in terms of linking nutrient deprivation to mitochondrial oxidant production through mitochondrial QC. A mitochondrion-mediated pathway was likely involved in RSV-SLN, thereby improving endurance performance. Overall, this study highlighted new possibilities for anti-physical-fatigue strategies.
Use of a Smartphone-Based Augmented Reality Video Conference App to Remotely Guide a Point of Care Ultrasound Examination
Authors: Ramsingh Davinder , Van Gorkom Cori , Holsclaw Matthew , Nelson Scott , De La Huerta Martin , Hinson Julian , Selleck Emilie
Published: October 2019

Reports on the use of various smartphone-based video conference applications to guide point-of-care ultrasound (POCUS) examinations in resource-limited settings have been described. However, the use of an augmented reality-enabled smartphone video conference application in this same manner has not been described. Presented is a case in which such as application was used to remotely guide a point of care ultrasound examination.
Dynamical Transitions in a One-Dimensional Katz–Lebowitz–Spohn Model
Authors: Pelizzola Alessandro , Pretti Marco , Puccioni Francesco
Published: October 2019

Dynamical transitions, already found in the high- and low-density phases of the Totally Asymmetric Simple Exclusion Process and a couple of its generalizations, are singularities in the rate of relaxation towards the Non-Equilibrium Stationary State (NESS), which do not correspond to any transition in the NESS itself. We investigate dynamical transitions in the one-dimensional Katz–Lebowitz–Spohn model, a further generalization of the Totally Asymmetric Simple Exclusion Process where the hopping rate depends on the occupation state of the 2 nodes adjacent to the nodes affected by the hop. Following previous work, we choose Glauber rates and bulk-adapted boundary conditions. In particular, we consider a value of the repulsion which parameterizes the Glauber rates such that the fundamental diagram of the model exhibits 2 maxima and a minimum, and the NESS phase diagram is especially rich. We provide evidence, based on pair approximation, domain wall theory and exact finite size results, that dynamical transitions also occur in the one-dimensional Katz–Lebowitz–Spohn model, and discuss 2 new phenomena which are peculiar to this model.
Performance of a Simple Energetic-Converting Reaction Model Using Linear Irreversible Thermodynamics
Authors: Chimal-Eguia J. C. , Paez-Hernandez R. , Ladino-Luna Delfino , Velázquez-Arcos Juan Manuel
Published: October 2019

In this paper, the methodology of the so-called Linear Irreversible Thermodynamics (LIT) is applied to analyze the properties of an energetic-converting biological process using simple model for an enzymatic reaction that couples one exothermic and one endothermic reaction in the same fashion as Diaz-Hernandez et al. (Physica A, 2010, 389, 3476–3483). We extend the former analysis to consider three different operating regimes; namely, Maximum Power Output (MPO), Maximum Ecological Function (MEF) and Maximum Efficient Power Function (MEPF), respectively. Based on the later, it is possible to generalize the obtained results. Additionally, results show analogies in the optimal performance between the different optimization criteria where all thermodynamic features are determined by three parameters (the chemical potential gap Δ = μ 1 − μ 4 R T , the degree of coupling q and the efficiency η ). This depends on the election that leads to more or less efficient energy exchange.
Multivariate Shortfall and Divergence Risk Statistics
Authors: Song Haiyan , Zeng Xianfu , Chen Yanhong , Hu Yijun
Published: October 2019

The aim of this paper is to construct two new classes of multivariate risk statistics, and to study their properties. We, first, introduce the multivariate shortfall risk statistics and multivariate divergence risk statistics. Then, their basic properties are studied, and their representation results are provided. Furthermore, their coherency is also characterized by means of the corresponding loss function. Finally, entropic risk statistics are given to illustrate the proposed new classes of multivariate risk statistics. The relationship between multivariate shortfall and divergence risk statistics is also discussed.
Critical Size of Secondary Nuclei Determined via Nucleation Theorem Reveals Selective Nucleation in Three-Component Co-Crystals
Authors: Gao Yang , Guo Baohua , Xu Jun
Published: October 2019

The critical size of the secondary nuclei plays an important role in determining the crystal growth rate. In the past, the Nucleation Theorem has been applied to determine the number of molecules in the critical nuclei of a single-component crystal via variation of the crystal growth rate with dilution by the non-crystallizable component. In this work, we extend the method to the three-component co-crystal poly (ethylene oxide)/urea/thiourea inclusion compound. The theoretical crystal growth kinetics were deduced and the dependence of the radial growth rate of the inclusion compound spherulites on the mass fraction of urea in urea/thiourea was measured. The results reveal that the secondary nuclei of the poly (ethylene oxide)/urea/thiourea inclusion compound consist mainly of ethylene oxide repeating units and urea molecules. We propose that only urea molecules and ethylene oxide repeating units are selected to form the secondary nuclei while co-crystallization of the three components happens at the lateral spreading stage. As a result, the composition of the critical secondary nuclei is different from that of the bulk inclusion compound crystals. The work is expected to deepen our understanding of the nucleation of multi-component co-crystals.
Improved Control of Grid-connected DFIG-based Wind Turbine using Proportional-Resonant Regulators during Unbalanced Grid
Authors: El Karkri Yassir , Rey-Boué Alexis B. , El Moussaoui Hassan , Stöckl Johannes , Strasser Thomas I.
Published: October 2019

The quality of power and current control are the greatest challenges of grid-connected wind farms during abnormal conditions. The negative- and positive-sequence components of the grid currents may be injected into a wind generation system during grid faults, which can affect the power stability and damage the wind system. The proposed work assures a low-voltage ride through capability of doubly-fed induction generator- based wind turbines under the grid voltage sag. A new technique to protect the wind system and to recompense the reactive power during failures of the utility grid according to the Spanish grid code is proposed. The control design is implemented to the power converters, and the grid current regulation is developed by using proportional-resonant regulators in a stationary two-phase (αβ) reference frame. The control performance is significantly validated by applying the real-time simulation for the rotor-side converter and the hardware in the loop simulation technique for the experiment of the generator’s grid-side converter control.
Investigating Primary Factors Affecting Electricity Consumption in Non-Residential Buildings Using a Data-Driven Approach
Authors: Cho Sooyoun , Lee Jeehang , Baek Jumi , Kim Gi-Seok , Leigh Seung-Bok
Published: October 2019

Although the latest energy-efficient buildings use a large number of sensors and measuring instruments to predict consumption more accurately, it is generally not possible to identify which data are the most valuable or key for analysis among the tens of thousands of data points. This study selected the electric energy as a subset of total building energy consumption because it accounts for more than 65% of the total building energy consumption, and identified the variables that contribute to electric energy use. However, this study aimed to confirm data from a building using clustering in machine learning, instead of a calculation method from engineering simulation, to examine the variables that were identified and determine whether these variables had a strong correlation with energy consumption. Three different methods confirmed that the major variables related to electric energy consumption were significant. This research has significance because it was able to identify the factors in electric energy, accounting for more than half of the total building energy consumption, that had a major effect on energy consumption and revealed that these key variables alone, not the default values of many different items in simulation analysis, can ensure the reliable prediction of energy consumption.
Contribution of Yeast Extract, Activated Carbon, and an Electrostatic Field to Interspecies Electron Transfer for the Bioelectrochemical Conversion of Coal to Methane
Authors: Piao Dong-Mei , Song Young-Chae , Oh Gyung-Geun , Kim Dong-Hoon , Bae Byung-Uk
Published: October 2019

The bioelectrochemical conversion of coal to methane was investigated in an anaerobic batch reactor containing yeast extract and activated carbon. In anaerobic degradation of coal, yeast extract was a good stimulant for the growth of anaerobic microorganisms, and activated carbon played a positive role. An electrostatic field of 0.67 V/cm significantly improved methane production from coal by promoting direct and mediated interspecies electron transfers between exoelectrogenic bacteria and electrotrophic methanogenic archaea. However, the accumulation of coal degradation intermediates gradually repressed the conversion of coal to methane, and the methane yield of coal was only 31.2 mL/g lignite, indicating that the intermediates were not completely converted to methane. By supplementing yeast extract and seed sludge into the anaerobic reactor, the intermediate residue could be further converted to methane under an electrostatic field of 0.67 V/cm, and the total methane yield of coal increased to 98.0 mL/g lignite. The repression of the intermediates to the conversion of coal to methane was a kind of irreversible substrate inhibition. The irreversible substrate inhibition in the conversion of coal to methane could be attenuated under the electrostatic field of 0.67 V/cm by ensuring sufficient biomass through biostimulation or bioaugmentation.
Control Strategy of BESS for Providing Both Virtual Inertia and Primary Frequency Response in the Korean Power System
Authors: Choi Woo Yeong , Kook Kyung Soo , Yu Ga Ram
Published: October 2019

As the battery energy storage system (BESS) has been considered to be a solution to the diminished performance of frequency response in the Korean power system, in which renewable energy resources (RESs) are expected to increase rapidly, this paper proposes a control strategy for providing both the virtual inertia and primary frequency response considering the MW-scale BESS installed by the Korea Electricity Power Corporation (KEPCO). The benefit of such a fast and flexible BESS can be maximized by the proposed control strategy for making it provide both the inertia and primary frequency response, which would be deficit with the increased RES. In the proposed control strategy, the state of charge (SOC) is maintained in the specific range in which the life cycle is maximized, the interference of SOC recovery by frequency control is minimized, the responding capacity for providing the virtual inertia response is maximized during the transient period, and the performance requirements for frequency response are satisfied. The effectiveness of the proposed strategy is verified by both Korean power system model-based simulation and on-site operations.
Dynamic Duty-Cycle MAC Protocol for IoT Environments and Wireless Sensor Networks
Authors: Kim Gayoung , Kang Jin-Gu , Rim Minjoong
Published: October 2019

This paper proposes a new protocol that can be used to reduce transmission delay and energy consumption effectively. This will be done by adjusting the duty-cycle (DC) ratio of the receiver node and the contention window size of the sender node according to the traffic congestion for various devices in the Internet of Things (IoT). In the conventional duty-cycle MAC protocol, the data transmission delay latency and unnecessary energy consumption are caused by a high collision rate. This is because the receiver node cannot sufficiently process the data of the transmitting node during the traffic peak time when the transmission and reception have the same duty-cycle ratio. To solve this problem, this paper proposes an algorithm that changes the duty-cycle ratio of the receiver and broadcasts the contention window size of the senders through Early Acknowledgment (E-ACK) at peak time and off/peak time. The proposed algorithm, according to peak and off/peak time, can transmit data with fewer delays and minimizes energy consumption.
Step-by-Step Small-Signal Modeling and Control of a Light Hybrid Electric Vehicle Propulsion System
Authors: Raga Carmen , Lázaro Antonio , Barrado Andrés , Martín-Lozano Alberto , Quesada Isabel
Published: October 2019

This paper develops step-by-step a complete electric model of a light hybrid electric vehicle propulsion system. This model includes the vehicle mass, the radius and mass of the wheels, the aerodynamic profile of the vehicle, the electric motor and the motor drive, among other elements. Each element of the model is represented by a set of equations, which lead to getting an equivalent electric circuit. Based on this model, the outer and inner loop compensators of the motor drive control circuit are designed to provide stability and a fast dynamic response to the system. To achieve this, the steady-state equations and the small-signal model of the equivalent electric circuit are also obtained. Furthermore, as these elements are the main load of the power distribution system of the fully electric and light hybrid electric vehicle, the input impedance model of the set composed of the input filter, the motor drive, the motor, and the vehicle is presented. This input impedance is especially useful to get the system stability of the entire power distribution system.
Probabilistic Methodology for Calculating PV Hosting Capacity in LV Networks Using Actual Building Roof Data
Authors: Grabner Miha , Souvent Andrej , Suljanović Nermin , Košir Andrej , Blažič Boštjan
Published: October 2019

There has been an increasing trend of integrating photovoltaic power plants (PVs). One of the important challenges for distribution system operators is to evaluate the total installed power of a PV that a particular network can host (or PV hosting capacity) while keeping voltage and element constraints within required limits. The major drawback of the existing methods for calculating PV hosting capacity is that they use the same installed power of the PV systems for all simulated PVs, as these methods do not use external data sources about building roofs. As a consequence, this has a significant impact on the final accuracy of the results. This paper presents a probabilistic methodology for calculating the PV hosting capacity in low voltage (LV) networks. The main contribution of this paper is the improved modeling of PV generation using actual building roof data when calculating the PV hosting capacity, as every building is treated according to its actual solar potential. Monte Carlo simulations with incorporated stochastic consumption and PV generation models are utilized for load flow calculations of the actual LV network. The simulation results presented in this paper prove that the proposed methodology increases the accuracy of the final PV hosting capacity calculations.
The Geochemistry of 1 ky Old Euxinic Sediments of the Western Black Sea
Authors: Duliu Octavian G. , Cristache Carmen I. , Bojar Ana-Voica , Oaie Gheorghe , Culicov Otilia-Ana , Frontasyeva Marina V. , Constantinescu Emil
Published: October 2019

To get more data on the geochemistry of Black Sea euxinic sediments, a 50-cm core was collected at a depth of 600 m on a Western Black Sea Continental Platform slope. The core contained unconsolidated sediments rich in cocoolithic ooze and mud. Epithermal Neutron and Prompt Gamma Activation Analysis were used to determine the content of nine major (Na, Mg, Al, Si, K, Ca, Ti, Mn, and Fe as oxides) and 32 trace elements (Cl, Sc, V, Cr, Co, Ni, Zn, As, Se, Br, Rb, Sr, Zr, Mo, Sn, Sb, Cs, Ba, La, Ce, Nd, Sm, Eu, Gd, Tb, Dy, Yb, Hf, Ta, W, Th, and U) with a precision varying between 3 and 9%. The core contained unconsolidated sediment rich in coccolithic ooze and mud. Previous 210 Pb geochronology suggests an age of ∼1 ky of considered sediments. Major components distribution showed that, except for Cl and Ca, the contents of all other elements are similar to Upper Continental Crust (UCC) and North American Shale Composite (NASC). The distribution of the 32 trace elements showed similarities to the UCC, except for redox-sensitive metals Fe, Se, Mo, and U, of which the significantly higher content reflects the presence of euxinic conditions during deposition. A chondrite normalized plot of nine rare earth elements indicated a similarity to UCC and NASC, suggesting a continental origin of sedimentary material.
Ethnic Variations in Nutritional Status among Preschool Children in Northern Vietnam: A Cross-Sectional Study
Authors: Le Thi Tuyet , Le Thi Thuy Dung , Do Nam Khanh , Nadezhda V. Savvina , Andrej M. Grjibovski , Nguyen Thi Trung Thu , Nguyen Thi Thanh Mai , Vu Thi Tuyen , Le Thi Huong , Nguyen Thi Thu Lieu , Duong Thi Anh Dao
Published: October 2019

(1) Background: Vietnam is a multiethnic country undergoing rapid economic development, the improvement in nutritional status in preschool children is not equally shared by all ethnic groups; (2) Methods: A cross-sectional study was performed from September–December 2018 on 16,177 children aged 24–60 months representing Kinh (n = 14421), Muong (n = 1307) and Tay (n = 449) ethnic groups. Prevalence of malnutrition, overweight, obesity and anthropometric indices were compared across ethnic groups, using WHO 2006 child growth standards; (3) Results: The prevalence of malnutrition among children of Kinh, Muong and Tay origins was 14.7%, 34.3% and 43.2%, respectively. The corresponding data for overweight was 5.5%, 2.7%, 2.2% and for obesity 2.8%, 0.8% and 0.4%, respectively. The prevalence of stunting remained the highest in three subtypes of malnutrition in all ethnic groups. Kinh children were heavier and taller than the other groups, while Muong children were taller than Tay children (p-value < 0.001); and (4) Conclusions: Malnutrition remains a major public health issue among children of minor ethnicities while overweight and obesity is an emerging challenge for the Kinh ethnic group. The results imply that a community-based intervention should be specific to ethnicity to reduce the gap in nutritional status between ethnic groups in Vietnam.
Metal (Pb, Cu, Cd, and Zn) Transfer along Food Chain and Health Risk Assessment through Raw Milk Consumption from Free-Range Cows
Authors: Miclean Mirela , Cadar Oana , Levei Erika Andrea , Roman Radu , Ozunu Alexandru , Levei Levente
Published: October 2019

Background: Metal transfer along the food chain has raised concerns about impacts on human health due to dietary exposure to low but chronic concentrations. Soil–forage–milk–consumer is a short food chain through which metals are able to reach an organism. Methods: Pb, Cu, Cd, and Zn were determined in water, soil, forage, and milk samples collected from free-range cattle farms situated near Baia Mare, Romania. The soil-to-forage (TFsf) and forage-to-milk (TFfm) transfer factors for metals and the health risk for three population groups (females, males, and children) through the consumption of milk containing low levels of metals were assessed. Results: TFsf indicated that the uptake capabilities of the metals from soil to forage were in the following order: Zn > Cd > Cu > Pb. TFfm indicated a lack of metal accumulation through forage ingestion. Estimated daily (EDI) and provisional tolerable weekly (PTWI) intake values revealed a minimal exposure of the population to those metals through milk consumption. A noncarcinogenic hazard index indicated that milk consumption from local markets does not pose any risk for human health; however, the average cancer risk showed a high potential carcinogenic risk. Conclusions: The consumption of milk produced by small local farmers does not pose noncarcinogenic risks. More extended studies should be carried out in order to identify the potential carcinogenic risk caused by the low levels of metals in the milk consumed.
Small Animal Veterinarians’ Perceptions, Experiences, and Views of Common Dog Breeds, Dog Aggression, and Breed-Specific Laws in the United States
Authors: Kogan Lori R. , Schoenfeld-Tacher Regina M. , Hellyer Peter W. , Oxley James A. , Rishniw Mark
Published: October 2019

Dog aggression directed towards humans is a common and serious behavioral and public health issue. This cross-sectional study was designed to gain insights into U.S. small animal veterinarians’ views and experiences with the most common dog breeds in the U.S., dog aggression, and breed-specific legislation. An electronic survey was distributed via email to an online veterinary community, and responses were summarized and compared by means of χ2 and Cochran–Mantel–Haenszel tests. Most respondents were concerned about the risks of dog bite injuries, but the majority were not in favor of banning specific breeds of dogs in order to enhance public safety. When participants rated the perceived bite risk associated with popular dog breeds, Chow Chows were perceived as the highest risk, with pit bull types categorized as a moderate risk. Golden Retrievers were seen as the most appropriate for families with children. Public education about animal behavior was the most frequently endorsed policy intervention to increase public safety. These findings suggest that most veterinarians feel that banning an entire dog breed is not an effective way to ensure human safety. Instead, most respondents endorsed alternative initiatives, such as public education and stricter leash laws, to reduce the risk of dog bites.
A Pilot Intervention Using Gamification to Enhance Student Participation in Classroom Activity Breaks
Authors: Beemer Lexie R. , Ajibewa Tiwaloluwa A. , DellaVecchia Gabriel , Hasson Rebecca E.
Published: October 2019

The purpose of this study was to determine the effect of adding game design elements (goal setting, feedback, and external rewards) to an evidence-based physical activity intervention to increase in-class physical activity participation (intensity of activity breaks performed). Nine third- through sixth-grade classrooms (n = 292 students) in one elementary-middle school in Detroit, Michigan (49% female, 95% nonwhite; 80% qualified for free/reduced lunch) participated in this 20-week intervention where teachers implemented 5 × 4 min moderate-to-vigorous activity breaks per day. Gamification of activity breaks occurred during weeks 13–20 of the intervention and included the use of game design elements and classroom goals for activity break intensity. Moderate-to-vigorous physical activity (MVPA) during activity breaks was measured via direct observation. There was a significant effect of intervention with a 27% increase in student MVPA participation during the gamified intervention weeks compared with the standard intervention weeks (p = 0.03). Gamification of activity breaks resulted in 55% (compared with 25% during the standard intervention) of students accumulating approximately 20 min of health-enhancing physical activity per day in their classroom. These findings provide preliminary evidence that gamifying activity breaks may be an important strategy for increasing student participation in classroom activity breaks.
Are Psychological Distress and Resilience Associated with Dietary Intake Among Australian University Students?
Authors: Whatnall Megan C. , Patterson Amanda J. , Siew Yu Yao , Kay-Lambkin Frances , Hutchesson Melinda J.
Published: October 2019

University students report unhealthy diets and experience poorer mental health than the general population. This study explores the association between psychological distress and resilience with dietary intake in a sample of Australian university students. Cross-sectional data from the University of Newcastle Student Healthy Lifestyle Survey 2017 were analysed. Psychological distress (Kessler Scale), resilience (Brief Resilience Scale) and fruit, vegetable, soft drink, takeaway food and breakfast intakes (short diet questions) were assessed. Socio-demographic (e.g., gender), student (e.g., undergraduate/postgraduate) and health characteristics (e.g., physical activity) were captured. Multivariate linear regression models explored associations between psychological distress and resilience with dietary intake, with adjustment for potential confounders. Analysis included 2710 students (mean age 26.9 ± 9.5 years, 30.4% male). In adjusted models, lower psychological distress was associated with higher fruit (β = −0.37, p = 0.001) and vegetable (β = −0.37, p < 0.001) serves/day, more frequent breakfast consumption (p < 0.001) and less frequent soft drink and takeaway food consumption (p < 0.001). Higher resilience was associated with higher fruit (β = 0.03, p = 0.022) and vegetable (β = 0.06, p < 0.001) serves/day, more frequent breakfast consumption (p = 0.005), and less frequent soft drink (p < 0.001) and takeaway food consumption (p = 0.001). These results highlight a potential link between psychological distress and resilience with diet, and that further research in this area is warranted.
Validation of the Hong Kong Chinese version of the Support Person’s Unmet Needs Survey—Short Form
Authors: Leung Doris Y. P. , Choy Yin-Ping , Ling Wai-Man , Yim Elaine , So Winnie K. W. , Chan Carmen W. H. , Mak Yim-Wah
Published: October 2019

This study describes the psychometric properties of a Hong Kong Chinese version of the short form of the Support Person’s Unmet Needs Survey (SPUNS-SF) for caregivers of patients with cancer. A convenience sample of 280 patient-caregiver dyads was recruited between April and June 2018. A subsample of 70 caregivers completed the survey again, two weeks later. A confirmatory factor analysis (CFA) examined the instrument’s factorial structure, ordinal alpha coefficients evaluated the internal consistency, and intra-class correlation coefficients (ICCs) assessed the test-retest reliability. Convergence validity was evaluated by the correlations with sleep disturbance and caregiver burden in caregivers. The Hong Kong Chinese version of the SPUNS-SF (SPUNS-SFHKC) had a high completion rate of 96.8% (271/280) among caregivers. The original five-factor model provided an acceptable fit with the data in the CFA. The ordinal alpha coefficients were 0.866–0.945, and the two-week test-retest reliabilities were 0.524–0.678. The correlations of the five domains of the SPUNS-SFHKC with caregiver burden were 0.257–0.446, and for sleep disturbance were 0.075–0.464. The SPUNS-SFHKC has a suitable factor structure and psychometric properties for use in assessing unmet supportive needs among Chinese caregivers of patients with cancer. The applicability of the instrument for long-term use still needs to be studied.
Air-Quality Assessment of On-Site Brick-Kiln Worker Housing in Bhaktapur, Nepal: Chemical Speciation of Indoor and Outdoor PM2.5 Pollution
Authors: Thygerson Steven M. , Beard John D. , House Marion J. , Smith Rilee L. , Burbidge Hunter C. , Andrus Kathryn N. , Weber Frank X. , Chartier Ryan , Johnston James D.
Published: October 2019

Brick workers and their families in Nepal generally live in poorly ventilated on-site housing at the brick kiln, and may be at higher risk for non-occupational exposure to fine particulate matter air pollution and subsequent respiratory diseases due to indoor and outdoor sources. This study characterized non-occupational exposure to PM2.5 by comparing overall concentrations and specific chemical components of PM2.5 inside and outside of brick workers’ on-site housing. For all samples, the geometric mean PM2.5 concentration was 184.65 μg/m3 (95% confidence interval: 134.70, 253.12 μg/m3). PM2.5 concentrations differed by kiln number (p = 0.009). Kiln number was significantly associated with 16 of 29 (55%) air pollutant, temperature, or relative humidity variables. There was not a significant interaction between kiln number and location of sample for PM2.5 (p = 0.16), but there was for relative humidity (p = 0.02) and temperature (p = 0.01). Results were qualitatively similar when we repeated analyses using indoor samples only. There was no difference in the chemical makeup of indoor and outdoor PM2.5 in this study, suggesting that outdoor PM2.5 air pollution easily infiltrates into on-site brick worker housing. Outdoor and indoor PM2.5 concentrations found in this study far exceed recommended levels. These findings warrant future interventions targeted to this vulnerable population.
An Exploration of Medical Education in Central and Southern China: Measuring the Professional Competence of Clinical Undergraduates
Authors: Cheng Xueyan , Chen Jing
Published: October 2019

Background: The cultivation and assessment of the professional competence of clinical undergraduates is essential to medical education. This study aimed to construct a scale to evaluate the professional competence of clinical undergraduates as well as its determinants. Methods: The competence scale was developed on the basis of four medical education standards, the literature, and expert interviews. A total of 288 undergraduates from two types of medical colleges in central and southeastern China were selected by a multistage sampling strategy. Factor analysis, correlation analysis, and internal consistency reliability were used to verify the validity and reliability of the scale. Results: A scale consisting of eight factors with 51 items was determined for factor analysis. Cronbach’α coefficients among the eight dimensions were over 0.800, with mean scores of 1.76, 1.38, 1.92, 1.54, 1.77, 1.25, 1.60, and 2.34. Clinical undergraduates with above average academic grades achieved a higher score in essential clinical knowledge (p < 0.05) and better professionalism was reported among females (p < 0.05). Conclusion: The competence scale showed excellent reliability and validity. Respondents in this study showed a moderate level of professional competence. This study could be a reference for medical educators and policy makers in order to improve medical education standards for clinical undergraduates in China and other countries with similar settings.
Performance Testing of istSOS under High Load Scenarios
Authors: Cannata Massimiliano , Antonovic Milan , Strigaro Daniele , Cardoso Mirko
Published: October 2019

In the last 20 years, a mainstream in Earth information and decision making has been drawn by the vision of the digital earth that calls for 3D representation, interoperability and modelling. In this context, the time dimension is essential but despite its importance, not many open standards and implementations are available. The Sensor Observation Service from the Open Geospatial Consortium is one of them and was specifically designed to collect, store and share timeseries of observations from sensors. To better understand the performance and limitation of one software implementation of this standard in real cases, this study executed a load testing of the istSOS application under a high load condition, characterized by a high number of concurrent users, in three cases mimicking existing monitoring networks. The results, in addition to providing reference values for future similar tests, show the general capacity of istSOS in meeting the INSPIRE quality of service requirements and in offering good performance with less than 500 concurrent users. When the number of concurrent users increases to 1000 and 2000, only 80% of the response times are below 30 seconds, performance that is unsatisfactory in most modern usages.
Using a Complex Network to Analyze the Effects of the Three Gorges Dam on Water Level Fluctuation in Poyang Lake
Authors: Ning Lixin , Zhou Yunkai , Cheng Changxiu , Ye Sijing , Shen Shi
Published: October 2019

Because the Three Gorges Dam (TGD) has disturbed the normal hydrological regime downstream, analyzing the influence of the TGD on water level fluctuation is of great importance to ecological planning. The distribution and dynamic of the water level before and after the TGD were analyzed using frequency distribution and a complex network. Frequency distribution was unimodal before the TGD, and the peak ranged from 13–15 m. Frequency distribution was bimodal after TGD and two peaks ranged from 9–10 m and 16–17 m. The number of days when the water level was above warning level was reduced, and it was increased when the water level was below the ecological level. Further, the TGD had little effect on the number of days of rapid water level rising, which mainly existed during the flood season. However, this imposed a greater influence on the number of days of rapid water level decline, which implies a weaker intensity of the recession process, along with a longer duration. Thirdly, in winter and spring, the water level after the TGD was lower than that before the TGD by approximately 1 m. In summer, the number of days when the water level was above warning level was reduced. In autumn, the frequency distribution changed from unimodal to bimodal. The TGD has the greatest influence during the winter, which resulted in a lower water level and more severe drought.
Automated Road Curb Break Lines Extraction from Mobile LiDAR Point Clouds
Authors: Gézero Luis , Antunes Carlos
Published: October 2019

In last decades, Mobile Light Detection And Ranging (LiDAR) systems were revealed to be an efficient and reliable method to collect dense and precise point clouds. The challenge now faced by researchers is the automatic object extraction from those point clouds, such as the curb break lines, which are essential to road rehabilitation projects and autonomous driving. Throughout this work, an efficient method to extract road curb break lines from mobile LiDAR point clouds is presented. The proposed method was based on the system working principles instead of an algorithmic application over the cloud as a mass of points. The point cloud was decomposed in the original sensor scan profiles. Then, a GPS epoch versus trajectory distance was used to eliminate most non-ground points. Finally, through a vertical monotone chain decomposition, candidate point arrays were created and the curb break lines are formed. The proposed method was shown to be able to avoid the occlusion effect caused by undergrowth. The method allows for distinguishing between right and left curbs and works on curved curbs. Both top and bottom tridimensional break lines were extracted. When compared with a reference manual method, in the tested dataset, the proposed method allowed for a decrease in the curb break lines extraction time from 25 min to less than 30 s. The extraction method provided completeness and correctness rates above 95% and 97%, respectively, and a quality value higher than 93%.
Road Extraction from Very High Resolution Images Using Weakly labeled OpenStreetMap Centerline
Authors: Wu Songbing , Du Chun , Chen Hao , Xu Yingxiao , Guo Ning , Jing Ning
Published: October 2019

Road networks play a significant role in modern city management. It is necessary to continually extract current road structure, as it changes rapidly with the development of the city. Due to the success of semantic segmentation based on deep learning in the application of computer vision, extracting road networks from VHR (Very High Resolution) imagery becomes a method of updating geographic databases. The major shortcoming of deep learning methods for road networks extraction is that they need a massive amount of high quality pixel-wise training datasets, which is hard to obtain. Meanwhile, a large amount of different types of VGI (volunteer geographic information) data including road centerline has been accumulated in the past few decades. However, most road centerlines in VGI data lack precise width information and, therefore, cannot be directly applied to conventional supervised deep learning models. In this paper, we propose a novel weakly supervised method to extract road networks from VHR images using only the OSM (OpenStreetMap) road centerline as training data instead of high quality pixel-wise road width label. Large amounts of paired Google Earth images and OSM data are used to validate the approach. The results show that the proposed method can extract road networks from the VHR images both accurately and effectively without using pixel-wise road training data.
Casting a Wider Net: Differentiating between Inner Nuclear Envelope and Outer Nuclear Envelope Transmembrane Proteins
Authors: Tingey Mark , Mudumbi Krishna C. , Schirmer Eric C. , Yang Weidong
Published: October 2019

The nuclear envelope (NE) surrounds the nucleus with a double membrane in eukaryotic cells. The double membranes are embedded with proteins that are synthesized on the endoplasmic reticulum and often destined specifically for either the outer nuclear membrane (ONM) or the inner nuclear membrane (INM). These nuclear envelope transmembrane proteins (NETs) play important roles in cellular function and participate in transcription, epigenetics, splicing, DNA replication, genome architecture, nuclear structure, nuclear stability, nuclear organization, and nuclear positioning. These vital functions are dependent upon both the correct localization and relative concentrations of NETs on the appropriate membrane of the NE. It is, therefore, important to understand the distribution and abundance of NETs on the NE. This review will evaluate the current tools and methodologies available to address this important topic.