bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting Adolescents’ Self-Objectification from Sexualized Video Game and Instagram Use: A Longitudinal Study
Authors: Skowronski Marika , Busching Robert , Krahé Barbara
Published: August 2020

A growing body of research has demonstrated negative effects of sexualization in the media on adolescents’ body image, but longitudinal studies and research including interactive and social media are scarce. The current study explored the longitudinal associations of adolescents’ use of sexualized video games (SVG) and sexualized Instagram images (SII) with body image concerns. Specifically, our study examined relations between adolescents’ SVG and SII use and appearance comparisons, thin- and muscular-ideal internalization, valuing appearance over competence, and body surveillance. A sample of 660 German adolescents (327 female, 333 male; Mage = 15.09 years) participated in two waves with an interval of 6 months. A structural equation model showed that SVG and SII use at Time 1 predicted body surveillance indirectly via valuing appearance over competence at Time 2. Furthermore, SVG and SII use indirectly predicted both thin- and muscular-ideal internalization through appearance comparisons at Time 1. In turn, thin-ideal internalization at Time 1 predicted body surveillance indirectly via valuing appearance over competence at Time 2. The results indicate that sexualization in video games and on Instagram can play an important role in increasing body image concerns among adolescents. We discuss the findings with respect to objectification theory and the predictive value of including appearance comparisons in models explaining the relation between sexualized media and self-objectification.
Efficacy and Safety of 100 Laparoscopy-Assisted Transgastric Endoscopic Retrograde Cholangiopancreatography Procedures in Patients with Roux-en-Y Gastric Bypass
Authors: Koggel Lieke M. , Wahab Peter J. , Robijn Rob J. , Aufenacker Theo J. , Witteman Bart P. L. , Groenen Marcel J. M. , Vrolijk Jan M.
Published: August 2020

Purpose

Laparoscopy-assisted transgastric endoscopic retrograde cholangiopancreatography (LAERCP) is an alternative for the anatomically challenging conventional ERCP in patients with a Roux-en-Y gastric bypass (RYGB) as it allows access to the biliary tree via the gastric remnant. We investigated the efficacy and safety of LAERCP.

Material and Methods

We retrospectively reviewed all charts from RYGB patients who underwent a LAERCP between January 2009 and August 2019 in a non-academic referral center for bariatric surgery. Patients who underwent pancreatic therapy were excluded. We collected demographic, clinical, and outcome data. An adverse event was defined as any complaint related to the LAERCP up to 30 days after the procedure and graded according to the ASGE lexicon.

Results

We identified 100 LAERCP in 86 patients with RYGB (70% female, median age 54 years). Same-session cholecystectomy was performed in 35 LAERCP (35%). The papilla of Vater was visualized in 100% of LAERCP with a therapeutic success rate of 94%. Stone extraction succeeded in 88.8% and sphincterotomy was performed in 96.7%. We identified 30 adverse events in 28 procedures, of which eight endoscopy-related, 14 laparoscopy-related, and eight non-specified (f.i. fever, allergic reaction). In total, six severe adverse events were reported concerning post-ERCP pancreatitis (n = 2), laparoscopy-related hemorrhage (n = 1), abscess (n = 1), shock (n = 1), and pneumonia (n = 1). No patient died due to LAERCP.

Conclusion

LAERCP is an effective and relatively safe procedure for biliary diseases in patients with RYGB.
Indoor environment quality and work performance in “green” office buildings in the Middle East
Authors: Elnaklah Rana , Fosas Daniel , Natarajan Sukumar
Published: August 2020

Globally, a primary concern is whether green office buildings perform as promised in terms of providing better indoor environment quality (IEQ) for employees, which may affect their satisfaction and work performance. In the Middle East, although there has been renewed interest in green building design, post occupancy evaluation of performance has never been conducted to-date, and evidence of actual occupant perception in green and non-green buildings is still ambiguous. Hence, we present the first study on IEQ performance in the Middle East. We show that Jordan can be taken as a representative example and systematically compare five “green” office buildings (representing 71% of all green-certified office buildings) against eight comparable conventional office buildings (CBs). Detailed bi-lingual survey data on perceived IEQ (n = 502) and work performance are accompanied by high-resolution continuous physical measurements of air temperature + relative humidity (n = 83) and CO2 concentrations (n = 21) with periodic measurements of mean radiant temperature and air speed, covering two typical summers and one typical winter. Results show both buildings types comply with design standards for indoor CO2 levels, while thermal comfort in green buildings is better than in CBs. However, CBs have a higher overall occupant satisfaction of IEQ. Work performance measured as absolute and relative absenteeism was slightly higher in CBs, with no significant differences in relative and absolute presenteeism between the two buildings types. These findings challenge the notion that green buildings improve occupant satisfaction and work performance over CBs and suggest the need for a better understanding of the performance-satisfaction gap.
Development of high-resolution melting PCR (HRM-PCR) assay to identify native fungal species associated with the wheat endosphere
Authors: Cłapa Tomasz , Mikołajczak Katarzyna , Błaszczyk Lidia , Narożna Dorota
Published: August 2020

Understanding the complexity and biodiversity of fungal communities associated with the wheat endosphere can facilitate the identification of novel strains that might be beneficial to the host plant. However, the differentiation and taxonomic classification of the endosphere-associated fungi with respect to various cultivars and plant organs are challenging, time-consuming, and expensive, even with the use of molecular techniques. In the present work, we describe a fast, simple, and low-cost method based on high-resolution melting PCR (HRM-PCR) for the identification and differentiation of wheat endogenous fungal isolates. Using this approach, we differentiated 28 fungal isolates, which belonged to five different genera, namely Alternaria, Penicillium, Epicoccum, Fusarium, and Trichoderma. Furthermore, the results of the study revealed that this method can allow large-scale screening of cultured samples.
Theoretical fundamentals of short pulse laser–metal interaction: A review
Authors: Li Xinxin , Guan Yingchun
Published: August 2020

Short and ultrashort pulse lasers offer excellent advantages in laser precision machining mainly because of their high pulse energy and low ablation threshold. The complex process of laser interaction with metals limits the in-depth investigation into laser ablation. Numerical simulation is important in the study of fundamental mechanisms. This review explores the start-of-the-art methods for the theoretical simulation of the laser ablation of metals, including plasma formation and expansion. Laser-induced period surface structures are also studied.
Mapping the epistemic arguments for religious toleration
Authors: BEAUCHAMP GILLES
Published: August 2020

In the literature on toleration, epistemic arguments are commonly equated with John Stuart Mill's fallibilism according to which toleration of opinions is a necessary means to the attainment of truth. This conflation does not capture the variety of those arguments and it results from the fact that a proper analysis of epistemic arguments for religious toleration and a systematic account of their different types are still lacking. The purpose of this article is to provide such an analysis and to argue that those arguments come in four different types, which I name fallibilism, religious belief-forming process, epistemic parity, and differentiated access to religious truth or divine message. I also offer a mapping of those different types of arguments using the standard epistemic notions of justification, truth, and belief coupled with a distinction of focus in the epistemological approach – whether individually or socially focused.
Chip-based ion chromatography (chip-IC) with a sensitive five-electrode conductivity detector for the simultaneous detection of multiple ions in drinking water
Authors: Li Xiaoping , Chang Honglong
Published: August 2020

The emerging need for accurate, efficient, inexpensive, and multiparameter monitoring of water quality has led to interest in the miniaturization of benchtop chromatography systems. This paper reports a chip-based ion chromatography (chip-IC) system in which the microvalves, sample channel, packed column, and conductivity detector are all integrated on a polymethylmethacrylate (PMMA) chip. A laser-based bonding technique was developed to guarantee simultaneous robust sealing between the homogeneous and heterogeneous interfaces. A five-electrode-based conductivity detector was presented to improve the sensitivity for nonsuppressed anion detection. Common anions (F−, Cl−, NO3−, and SO42−) were separated in less than 8 min, and a detection limit (LOD) of 0.6 mg L−1 was achieved for SO42−. Tap water was also analyzed using the proposed chip-IC system, and the relative deviations of the quantified concentration were less than 10% when compared with that a commercial IC system.
Nanovolcano microelectrode arrays: toward long-term on-demand registration of transmembrane action potentials by controlled electroporation
Authors: Desbiolles Benoît X. E. , de Coulon Etienne , Maïno Nicolas , Bertsch Arnaud , Rohr Stephan , Renaud Philippe
Published: August 2020

Volcano-shaped microelectrodes (nanovolcanoes) functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular access. These nanovolcanoes exhibit recording characteristics similar to those of state-of-the-art micro-nanoelectrode arrays that use electroporation as an insertion mechanism. In this study, we investigated whether the use of electroporation improves the performance of nanovolcano arrays in terms of action potential amplitudes, recording durations, and yield. Experiments with neonatal rat cardiomyocyte monolayers grown on nanovolcano arrays demonstrated that electroporation pulses with characteristics derived from analytical models increased the efficiency of nanovolcano recordings, as they enabled multiple on-demand registration of intracellular action potentials with amplitudes as high as 62 mV and parallel recordings in up to ~76% of the available channels. The performance of nanovolcanoes showed no dependence on the presence of functionalized nanopatterns, indicating that the tip geometry itself is instrumental for establishing a tight seal at the cell–electrode interface, which ultimately determines the quality of recordings. Importantly, the use of electroporation permitted the recording of attenuated cardiomyocyte action potentials during consecutive days at identical sites, indicating that nanovolcano recordings are nondestructive and permit long-term on-demand recordings from excitable cardiac tissues. Apart from demonstrating that less complex manufacturing processes can be used for next-generation nanovolcano arrays, the finding that the devices are suitable for performing on-demand recordings of electrical activity from multiple sites of excitable cardiac tissues over extended periods of time opens the possibility of using the devices not only in basic research but also in the context of comprehensive drug testing.
Capacitive micromachined ultrasound transducers for intravascular ultrasound imaging
Authors: Wang Jiaqi , Zheng Zhou , Chan Jasmine , Yeow John T. W.
Published: August 2020

Intravascular ultrasound (IVUS) is a burgeoning imaging technology that provides vital information for the diagnosis of coronary arterial diseases. A significant constituent that enables the IVUS system to attain high-resolution images is the ultrasound transducer, which acts as both a transmitter that sends acoustic waves and a detector that receives the returning signals. Being the most mature form of ultrasound transducer available in the market, piezoelectric transducers have dominated the field of biomedical imaging. However, there are some drawbacks associated with using the traditional piezoelectric ultrasound transducers such as difficulties in the fabrication of high-density arrays, which would aid in the acceleration of the imaging speed and alleviate motion artifact. The advent of microelectromechanical system (MEMS) technology has brought about the development of micromachined ultrasound transducers that would help to address this issue. Apart from the advantage of being able to be fabricated into arrays with lesser complications, the image quality of IVUS can be further enhanced with the easy integration of micromachined ultrasound transducers with complementary metal-oxide-semiconductor (CMOS). This would aid in the mitigation of parasitic capacitance, thereby improving the signal-to-noise. Currently, there are two commonly investigated micromachined ultrasound transducers, piezoelectric micromachined ultrasound transducers (PMUTs) and capacitive micromachined ultrasound transducers (CMUTs). Currently, PMUTs face a significant challenge where the fabricated PMUTs do not function as per their design. Thus, CMUTs with different array configurations have been developed for IVUS. In this paper, the different ultrasound transducers, including conventional-piezoelectric transducers, PMUTs and CMUTs, are reviewed, and a summary of the recent progress of CMUTs for IVUS is presented.
T-cell tumour exclusion and immunotherapy resistance: a role for CAF targeting
Authors: Hanley Christopher J. , Thomas Gareth J.
Published: August 2020

Recent studies have highlighted a major role for cancer-associated fibroblasts (CAFs) in promoting immunotherapy resistance by excluding T cells from tumours. Recently, we showed that CAFs can be effectively targeted by inhibiting the enzyme NOX4; this ‘normalises’ CAFs and overcomes immunotherapy resistance. Here we discuss our study and other strategies for CAF targeting.
Predictive Models for Very Preterm Birth: Developing a Point-of-Care Tool
Authors: Hebert Courtney L. , Nattino Giovanni , Gabbe Steven G. , Gabbe Patricia T. , Benedict Jason , Philips Gary , Lemeshow Stanley
Published: August 2020

Objective The objective of this study was to create three point-of-care predictive models for very preterm birth using variables available at three different time points: prior to pregnancy, at the end of the first trimester, and mid-pregnancy.

Study Design This is a retrospective cohort study of 359,396 Ohio Medicaid mothers from 2008 to 2015. The last baby for each mother was included in the final dataset. Births prior to 22 weeks were excluded. Multivariable logistic regression was used to create three models. These models were validated on a cohort that was set aside and not part of the model development. The main outcome measure was birth prior to 32 weeks.

Results The final dataset contained 359,396 live births with 6,516 (1.81%) very preterm births. All models had excellent calibration. Goodness-of-fit tests suggested strong agreement between the probabilities estimated by the model and the actual outcome experience in the data. The mid-pregnancy model had acceptable discrimination with an area under the receiver operator characteristic curve of approximately 0.75 in both the developmental and validation datasets.

Conclusion Using data from a large Ohio Medicaid cohort we developed point-of-care predictive models that could be used before pregnancy, after the first trimester, and in mid-pregnancy to estimate the probability of very preterm birth. Future work is needed to determine how the calculator could be used to target interventions to prevent very preterm birth.

Key Points
A Standardized Protocol for Nonoperative Management of Complicated Appendicitis in Children Reduces Resource Utilization
Authors: Pennell Christopher , Meckmongkol Teerin , Prasad Rajeev , Ciullo Sean , Arthur III Lindsay Grier , Lindholm Erika , Grewal Harsh
Published: August 2020

Introduction To standardize care and reduce resource utilization, we implemented a standardized protocol (SP) for the nonoperative treatment of complicated appendicitis.

Materials and Methods We conducted a prospective, historically controlled, study of patients <21 years with complicated appendicitis managed nonoperatively using an SP from January 2017 to November 2018. The primary outcomes included length of stay (LOS), antibiotic days, peripheral inserted central catheter (PICC) utilization, discharge on intravenous antibiotics, and predischarge imaging. Secondary outcomes were protocol adherence and the rates of adverse events (AE) including return to emergency department (ED), readmission, failure of nonoperative treatment, and interval appendectomy complications.

Results Protocol adherence was 67.9%. In total, 741 children were treated for appendicitis of which 58 (30 pre-SP and 28 post-SP) were treated nonoperatively for complicated appendicitis at presentation. Patients were well matched for age, admission white blood cell, sex, body mass index, race, and the proportion requiring percutaneous drainage. After implementing the SP, fewer children had PICCs (100.0 vs. 57.1%, p ≤ 0.001), fewer were discharged on intravenous antibiotics (90.0 vs. 42.9%, p < 0.001), and total antibiotic days were reduced (14.0 vs. 10.0, p = 0.006). There was no difference in LOS (5.5 vs. 6.0 days, p = 0.790) or the proportion undergoing ultrasound (36.7 vs. 39.3%, p = 0.837) or computed tomography scan (16.7 vs. 3.6%, p = 0.195) prior to discharge. There were nonsignificant trends toward reduced AEs (46.7 vs. 35.7%, p = 0.397), returns to ED (40.0 vs. 28.6%, p = 0.360), and readmissions (26.7 vs. 17.9%, p = 0.421). The proportion failing nonoperative treatment (10.0 vs. 3.6%, p = 0.612) and experiencing complications of interval appendectomy (3.3 vs. 3.6%, p = 0.918) were not significantly different.

Conclusion Implementing an SP for treating complicated appendicitis nonoperatively reduced resource utilization without negatively affecting clinical outcomes.
The Clinical Spectrum of Patients of Horizontal Semicircular Canal Benign Paroxysmal Positional Vertigo Attending an Otoneurology Center in South Rajasthan, India and Their Response to Appropriate Repositioning Maneuvers at a Short-Term Follow-Up
Authors: Vats Ajay Kumar
Published: August 2020

Introduction This article aims to study the clinical spectrum of 20 consecutive patients diagnosed with horizontal semicircular canal benign paroxysmal positional vertigo (HSC-BPPV) attending an otoneurology center in Udaipur, Rajasthan, India, over a period of 11 months and their response to appropriate repositioning maneuvers evaluated at short-term follow-up of 1 hour and 24 hours.

Study Design This is a nonrandomized prospective interventional study.

Materials and Methods Twenty patients with unilateral HSC-BPPV were treated with a session of an appropriate repositioning maneuver (Gufoni maneuver or barbecue roll maneuver for the geotropic variant of HSC-BPPV [geo-HSC-BPPV]; Appiani maneuver or barbecue roll maneuver or head-shaking maneuver for the apogeotropic variant of the HSC-BPPV [apo-HSC-BPPV]). Patients were followed up twice (at 1 hour and 24 hours) and audited by a supine roll test with questioning for the absence or presence of concomitant vertigo.

Results At the 1-hour follow-up, 78.57% (11/14) patients of geo-HSC-BPPV treated with Gufoni maneuver recovered, and 66.67% (4/6) patients of apo-HSC-BPPV treated with some form of physical therapy recovered. The recovery was maintained at 24 hours’ follow-up in both groups.

Conclusion To the best of the author’s knowledge, no such study on patients of HSC-BPPV has been reported from India hitherto. Gufoni maneuver is an effective and safe treatment for the geo-HSC-BPPV with a recovery rate of 78.57% (11/14) at a short-term follow-up. The physical therapy for the apo-HSC-BPPV must be tailored according to the purported site of pathology which cannot be precisely predetermined most of the times.
Intraoperative Thrombophilia-Associated Thrombosis of Both Saphenous Veins during Harvesting for Coronary Artery Bypass Grafting
Authors: Mazur Piotr , Ząbczyk Michał , Litwinowicz Radosław , Natorska Joanna , Kapelak Bogusław
Published: August 2020

Introduction Intraoperative thrombosis of saphenous veins (SV) during open harvesting is very rare.

Case Report We present a case of a 60-year-old male patient with multivessel coronary artery disease and a history of a non-ST elevation acute coronary syndrome, and type-2 diabetes mellitus admitted for coronary artery bypass grafting, in whom bilateral intraoperative SV thrombosis occurred during graft harvesting. Routine thrombophilia screening showed no abnormalities and cancer was excluded. Compared with healthy controls, we observed prolonged fibrin clot lysis time and increased thrombin generation reflected by endogenous thrombin potential. Scanning electron microscopy of the thrombosed material revealed compact and thick fibrin layer on the clot surface with a solid mass of unusually compressed platelets and erythrocytes underneath. The patient was tested for fibrinogen and factor (F) XIII polymorphisms, and was found to be heterozygous for β-fibrinogen HaeIII (-455G > A) and FXIII Val34Leu (100G > T).

Conclusion β-fibrinogen HaeIII and FXIII Val34Leu polymorphisms are reflected in reduced clot permeability and susceptibility to lysis, and might contribute to intraoperative SV thrombosis during vascular grafting procedures. Carriers of those are at risk of primary venous graft failure after bypass procedures.
Analyzing Burmese refugee children’s multimodal work
Authors: Cun Aijuan
Published: August 2020

Researchers have investigated how family literacy practices can effectively support children’s literacy development in school. However, few studies have explored the lived experiences of Burmese refugee families in the United States. Utilizing a social semiotics multimodal perspective, this qualitative study examines how two Burmese refugee children made meaning by blending different modes. The data sources include video recordings, artifacts, and interviews. The findings illustrate three major themes that span time and space: family past experiences across global contexts, representation of current life experiences in the United States, and family beliefs carried across global contexts and Gawa’s dream for the future. The findings also show that the participants drew upon multimodal semiotic resources to create and share family storybooks. Implications include the importance of integrating multimodal perspectives into classroom learning and the possibilities of bridging home and school literacy.
Teachers’ collaboration-mediated practices in meeting the needs of refugee EAL students in the Victorian secondary science classroom
Authors: Nguyen Minh Hue
Published: August 2020

This paper examines the collaborative practices of an English as an additional language (EAL) teacher and a content area teacher in meeting the needs of refugee students in a secondary science classroom. The study is based in Victoria, Australia. Drawing on a sociocultural perspective on mediated teacher work, the study gathered qualitative data primarily through two group interviews with the teachers in the middle and at the end of the school term. A secondary source of data includes relevant documents namely teaching materials, students’ work, and teachers’ notes. Content analysis of data shows two broad collaborative practices, including joint lesson planning and resources development and joint teaching. These collaborative practices were found to create structured mediational spaces that enabled them to effectively work together to meet the needs of refugee EAL students in their class. The study generates implications for content and EAL teachers in collaboratively addressing the needs of refugees in mainstream classrooms and for school leadership in supporting EAL and content teachers in this process.
Agency and pedagogy in literacy education
Authors: Jeong Heeok
Published: August 2020

This longitudinal ethnographic case study examines, from a sociocultural perspective, how a White female English as a second language teacher of immigrant adolescents enacted agency toward culturally sustaining pedagogy in her classroom, school, and community. The teacher demonstrated her agency in her personal and professional spaces by (a) re-contextualizing curriculum and instruction with students, (b) engaging in daily conversations with students, (c) recruiting community members to participate in class, (d) constructing an ELL teacher community, (e) visiting students’ homes and involving herself in refugee communities, and (f) performing community services with students. This study provides significant implications about how teachers, who will be working in classrooms and schools and encountering deficit discourses about immigrant students and standardization forces, can create agentive pedagogical spaces where the linguistic, cultural, and community resources of immigrant students are identified, understood, centered, and can connect those resources with classroom and community practices.
Enhancing Asian immigrants’ and international students’ learning
Authors: Yeh Ellen , Jaiswal-Oliver Monika , Sharma Rashmi , Wan Guofang
Published: August 2020

With the rapid growth of Asian immigrants in American educational settings, this mixed method study examined whether a professional development program on cultural awareness and pedagogy for faculty members would impact their knowledge and skills in working with Asian immigrants to ensure these students’ retention and success. This study, therefore, assessed the effectiveness and the impact of the program designed to address the needs of Asian immigrants. Guided and supported by the constructivism learning theory (Vygotsky, 1980), the professional development program applied theories of intercultural communication training (Bennett & Salonen, 2004). A mixed method of inquiry involved surveys with ten faculty members and 16 students, in-depth interviews, classroom observations, and document analysis. Faculty members attended the workshops during the first year of students’ enrollment in their classes. The data stem from a longitudinal study conducted throughout the three years of students’ enrollment in the institution. The results established a probable relationship between the workshops and faculty members’ success in working with Asian immigrants.
Why is reproductive isolation between intraspecific cytotypes so important? A commentary on: ‘Strong post-pollination interactions govern cytotype isolation in a tetraploid–octoploid contact zone’
Authors: Čertner Martin
Published: August 2020

This article comments on:

Mariana Castro1, João Loureiro, Brian C. Husband and Sílvia Castro, The role of multiple reproductive barriers: strong post-pollination interactions govern cytotype isolation in a tetraploid–octoploid contact zone, Annals of Botany, Volume 126, Issue 6, 2 November 2020, Pages 991–1003, https://doi.org/10.1093/aob/mcaa084
Sex-Dependent Effects of theAPOEɛ4 Allele on Behavioral Traits and White Matter Structures in Young Adults
Authors: Takeuchi Hikaru , Tomita Hiroaki , Browne Ryan , Taki Yasuyuki , Kikuchi Yoshie , Ono Chiaki , Yu Zhiqian , Nouchi Rui , Yokoyama Ryoichi , Kotozaki Yuka , Nakagawa Seishu , Sekiguchi Atsushi , Iizuka Kunio , Hanawa Sugiko , Araki Tsuyoshi , Miyauchi Carlos Makoto , Sakaki Kohei , Nozawa Takayuki , Ikeda Shigeyuki , Yokota Susumu , Magistro Daniele , Sassa Yuko , Kawashima Ryuta
Published: August 2020

The APOE ɛ4 allele is associated with a risk of Alzheimer’s disease in the elderly, with the association being pronounced in females. Conversely, findings of the effects of the APOE ɛ4 allele in young adults are mixed. Here, we investigated the sex–genotype interaction effects of the APOE ɛ4 allele on cognitive functions as well as brain structures among 1258 young adults. After adjusting for multiple comparisons, there were significant effects of the interaction between sex and the number of APOE ɛ4 allele on some speed tasks (e.g., simple processing speed tasks and the reverse Stroop task) as well as on regional white matter volume (rWMV). The observed sex–genotype interaction conferred better cognitive performance and greater rWMV in the anterior frontal and precentral white matter areas in females having more APOE ɛ4 alleles and reduced rWMV in the same areas in male having more APOE ɛ4 alleles. These findings support the long-debated antagonistic pleiotropic effects of the APOE ɛ4 allele in females.
Short-term exposure to unmanned aerial vehicles does not alter stress responses in breeding tree swallows
Authors: Scholten Bradley D , Beard Abigail R , Choi Hyeryeong , Baker Dena M , Caulfield Margaret E , Proppe Darren S
Published: August 2020

Recent studies suggest that visual and acoustic anthropogenic disturbances can cause physiological stress in animals. Human-induced stress may be particularly problematic for birds as new technologies, such as drones, increasingly invade their low-altitude air space. Although professional and recreational drone usage is increasing rapidly, there is little information on how drones affect avian behavior and physiology. We examined the effects of drone activity on behavior and physiology in adult, box-nesting tree swallows (Tachycineta bicolor). Specifically, we monitored bird behavior during drone flights and in response to a control object and measured telomere lengths and corticosterone levels as indicators of longer-term physiological stress. We predicted that drone-exposed tree swallows would habituate behaviorally after multiple flights, but that telomeres would shorten more quickly and that baseline corticosterone levels would be altered. One significant and two strong, non-significant trends in behavioral assays indicated that adult swallows acted more aggressively towards drone presence compared to a control object, but were slower to approach the drone initially. Swallows were also more reluctant to use nest boxes during drone activity. Tree swallows habituated to drone presence as expected, although the rate of habituation often did not differ between drone-exposed and control groups. Contrary to our prediction, drone activity did not affect telomere length, corticosterone levels, body mass or fledging rates. Overall, our results indicate that a small number of short, targeted, drone flights do not impact tree swallow health or productivity differently than a non-invasive control object. Minor behavioral differences suggest that increasing the frequency of drone use could impact this species. We provide some of the first results addressing how drone activity alters behavioral, physiological and molecular responses to stress in songbirds. A better understanding of these impacts will allow ecologists to make more informed decisions on the use and regulation of new drone technologies.
Diagnostic challenge in primary cardiac lymphoma: a case report
Authors: Tanking Chonthicha , Ratanapo Supawat
Published: August 2020

Background

Primary cardiac lymphoma is an extra-nodal non-Hodgkin’s lymphoma, which usually responds well to chemotherapy. The disease has high mortality rate unless it is recognized and treated in time. Tissue pathology is crucially the diagnosis gold standard for treatment plan. This is a case report of an elderly female who presented with a huge right-sided cardiac tumour obstructing tricuspid flow.

Case summary

An 81-year-old Asian female presented with clinical right-sided heart failure. Echocardiogram showed a large mass compressing and obliterating the right atrium. Trans-jugular tissue biopsy was performed. Initial pathology report was consistent with an angiosarcoma, based on an expression of Fli-1 (Friend leukaemia virus integration 1) from immunohistochemical staining. She died shortly after refusal to surgery. Autopsy was performed with diagnosis change to a diffuse large B-cell lymphoma (DLBCL) after tissue pathology.

Discussion

Primary cardiac lymphoma is extremely rare. Adequate tissue and proper immunohistochemical staining are mandatory for treatment plan. Besides an angiosarcoma, DLBCL should be considered in the differential diagnosis of Fli-1 positive tissue cardiac mass.
Prosthetic graft replacement of a large subclavian aneurysm in a child with Loeys–Dietz syndrome: a case report
Authors: Sobh Mohamed , Voges Inga , Attmann Tim , Scheewe Jens
Published: August 2020

Background

Loeys–Dietz syndrome (LDS) is a genetic connective tissue disorder, which is characterized by rapid development of aortic and peripheral arterial aneurysms. Loeys–Dietz syndrome has some overlapping phenotypic features with other inherited aortopathies such as Marfan syndrome. However, LDS has a more aggressive vascular course with patient morbidity and mortality occurring at an early age.

Case summary

We present the rare case of an 11-year-old girl with LDS who underwent valve sparing aortic root replacement at the age of 2.9 years with good results. She had routine follow-up cardiovascular magnetic resonance imaging and was found to have a large aneurysm of the right subclavian artery. After multidisciplinary team discussion, successful surgical resection with prosthetic graft replacement of the right subclavian artery was performed.

Discussion

This case illustrates that large aneurysms of aortic branches can already develop in childhood and underlines the need for frequent follow-ups including cross-sectional imaging and multidisciplinary team management.
Thoracic duct decompression and jugular vein banding—an effective treatment option for protein-losing enteropathy and plastic bronchitis in severe failing Fontan circulation: a case report
Authors: Bauer Christoph , Mair Roland , Mair Rudolf , Tulzer Gerald
Published: August 2020

Background

Plastic bronchitis (PB) and protein-losing enteropathy (PLE) are devastating complications after Fontan palliation that lead to uncontrolled loss of protein-rich lymphatic fluid into extra lymphatic compartments. Decompression of the thoracic duct is a new treatment option that effectively restores lymphatic system integrity by redirecting lymphatic flow into the low-pressure levels of the common atrium.

Case summary

We report a patient with severe failing Fontan circulation where surgical thoracic duct decompression leads to resolution of PLE and PB symptoms but worsening hypoxaemia that could be managed with banding of internal jugular vein.

Discussion

Thoracic duct decompression in patients with failing Fontan circulation can be a simple and effective treatment for PLE and PB. Hypoxaemia may occur but can be managed with banding of internal jugular vein.
Percutaneous closure of accidental left atrium puncture: a case report
Authors: Sá Mendes Gustavo , De Araújo Gonçalves Pedro , Madeira Sérgio , Oliveira Paulo
Published: August 2020

Background

Minimally invasive alternatives to surgical closure of cardiac perforations are a recognized need, especially in critically ill patients in whom predicted surgical mortality is prohibitive. To the best of our knowledge, this is the first reported case of an iatrogenic left atrium (LA) puncture closed with a plug-based vascular closure device (VCD).

Case summary

During a palliative right-sided thoracentesis on a 73-year-old woman, with end-stage heart failure due to rheumatic valvular heart disease, an accidental puncture and insertion of a central venous catheter into an aneurysmatic LA occurred. This complication was successfully managed percutaneously, under transthoracic echocardiographic guidance, after cardiac computed tomography planning, using a plug-based VCD.

Discussion

This case demonstrates the possible utility of plug-based devices for iatrogenic LA perforation closure, when surgical risk is deemed prohibitive.
Cerebral venous thrombosis as a rare thromboembolic complication of celiac disease: a case report
Authors: Bouziane Maha , Arous Salim , Habbal Rachida
Published: August 2020

Background 

Celiac disease is a digestive inflammatory syndrome with several complications. It is associated with coagulation and platelets abnormalities leading to thromboembolic events. Cerebral venous thrombosis is an exceptional localization of thrombosis in celiac disease and could be life-threatening.

Case summary 

A 17-year-old female patient with history of celiac disease and not following a gluten-free diet, checked in to the emergency department for a sudden, 2-week-old, and deteriorating, onset of intense headache and muscle weakness. The cerebral computed tomography-scan showed bilateral fronto-parietal hypodensity with micro-bleeds. We investigated using a cerebral magnetic resonance imaging that revealed superior longitudinal sinus thrombosis and right transverse and sigmoid sinuses thrombosis, along with right haematoma and ischaemic areas. The patient was prescribed anticoagulation therapy. Follow-ups over a 2-year period confirmed a favourable outcome and a complete regression of symptoms.

Discussion 

Evolution of celiac disease could be associated with several complications. Eighty-five percent of patients is potentially exposed to thromboembolic events due to the hypercoagulability state of the disease and different coagulation and fibrinolysis abnormalities (e.g. hyperhomocysteinaemia, protein C and S deficiencies, vitamin K and B deficiencies). Cerebral venous thrombosis is a rare thromboembolic localization. Anticoagulation is efficient in most cases though endovascular treatment might be required.
Efficacy of entry exclusion strategy for DeBakey type III retrograde Stanford type A acute aortic dissection
Authors: Inoue Yosuke , Matsuda Hitoshi , Matsuo Jiro , Shijo Takayuki , Omura Atsushi , Seike Yoshimasa , Uehara Kyokun , Sasaki Hiroaki , Kobayashi Junjiro
Published: August 2020

OBJECTIVES

Resection of a primary entry tear is essential for the treatment of Stanford type A acute aortic dissection (AAAD). In DeBakey type III retrograde AAAD (DBIII-RAAAD), resection of the primary entry tear in the descending aorta is sometimes difficult. The frozen elephant trunk technique and thoracic endovascular aortic repair (TEVAR) enable the closure of the primary entry in the descending aorta. The aim of this study was to investigate the efficacy of resection or closure of primary entry, i.e. entry exclusion, in patients with DeBakey type III retrograde-AAAD.

METHODS

The medical records of 654 patients with AAAD who underwent emergency surgery between January 2000 and March 2019 were retrospectively reviewed, and 80 eligible patients with DeBakey type III retrograde-AAAD were divided into the excluded (n = 50; age, 62 ± 12 years) and residual (n = 30; age, 66 ± 14 years) groups according to postoperative computed tomography angiographic data of the false lumen around the primary entry. The excluded group was defined as having a postoperative false lumen at the level of the elephant trunk or thrombosis of the endograft including primary entry. Patients with early false lumen enhancement around the elephant trunk or an unresected primary entry tear after isolated hemiarch replacement were included in the residual group. The early and long-term surgical outcomes were compared between the groups.

RESULTS

The in-hospital mortality rate was 8% (6/80), with no significant difference observed between the excluded and the residual groups (10% and 7%, respectively; P > 0.99). Ninety-five percentage of the patients (20/21) achieved entry exclusion with stent grafts including the frozen elephant trunk procedure and TEVAR. Spinal cord ischaemia was not observed in either group. The cumulative overall survival at 5 years was comparable between the 2 groups (76% and 81% in the excluded and residual groups, respectively; P = 0.93). The 5-year freedom from distal aortic reoperation rate was significantly higher in the excluded group (97%) than in the residual group (97% vs 66%; P = 0.008).

CONCLUSIONS

Not only resection but also closure using the entry exclusion approach for DeBakey type III retrograde-AAAD utilizing new technologies including the frozen elephant trunk technique and TEVAR might mitigate dissection-related reoperations.
DNA damage bypass pathways and their effect on mutagenesis in yeast
Authors: Arbel Matan , Liefshitz Batia , Kupiec Martin
Published: August 2020

What is the origin of mutations? In contrast to the naïve notion that mutations are unfortunate accidents, genetic research in microorganisms has demonstrated that most mutations are created by genetically encoded error-prone repair mechanisms. However, error-free repair pathways also exist, and it is still unclear how cells decide when to use one repair method or the other. Here, we summarize what is known about the DNA damage tolerance mechanisms (also known as post-replication repair) for perhaps the best-studied organism, the yeast Saccharomyces cerevisiae. We describe the latest research, which has established the existence of at least two error-free and two error-prone inter-related mechanisms of damage tolerance that compete for the handling of spontaneous DNA damage. We explore what is known about the induction of mutations by DNA damage. We point to potential paradoxes and to open questions that still remain unanswered.
Divergent Evolution of Mutation Rates and Biases in the Long-Term Evolution Experiment with Escherichia coli
Authors: Maddamsetti Rohan , Grant Nkrumah A
Published: August 2020

All organisms encode enzymes that replicate, maintain, pack, recombine, and repair their genetic material. For this reason, mutation rates and biases also evolve by mutation, variation, and natural selection. By examining metagenomic time series of the Lenski long-term evolution experiment (LTEE) with Escherichia coli (Good BH, McDonald MJ, Barrick JE, Lenski RE, Desai MM. 2017. The dynamics of molecular evolution over 60,000 generations. Nature 551(7678):45–50.), we find that local mutation rate variation has evolved during the LTEE. Each LTEE population has evolved idiosyncratic differences in their rates of point mutations, indels, and mobile element insertions, due to the fixation of various hypermutator and antimutator alleles. One LTEE population, called Ara+3, shows a strong, symmetric wave pattern in its density of point mutations, radiating from the origin of replication. This pattern is largely missing from the other LTEE populations, most of which evolved missense, indel, or structural mutations in topA, fis, and dusB—loci that all affect DNA topology. The distribution of mutations in those genes over time suggests epistasis and historical contingency in the evolution of DNA topology, which may have in turn affected local mutation rates. Overall, the replicate populations of the LTEE have largely diverged in their mutation rates and biases, even though they have adapted to identical abiotic conditions.
Brazilian GIs Landscape: From the TRIPS Commitments to the Real World, What Was Achieved, What Is Yet to be Faced?
Authors: Carls Suelen
Published: August 2020

After 25 years of the TRIPS Agreement, it is time to look at the Brazilian GI system and how it has developed since its establishment. GIs under TRIPS might not be the universal consensus, but they formed the starting point for the Brazilian GI system, and since 1996 GIs are expressly mentioned as such in domestic legislation. The article analyses the Brazilian GI journey from TRIPS to today’s framework, and points out the challenges that are yet to be faced at domestic, regional and international level.
Impacts of the Percentage of Basal Core Promoter Mutation on the Progression of Liver Fibrosis After Hepatitis B e Antigen Seroconversion
Authors: Wu Jia-Feng , Chang Kai-Chi , Ni Yen-Hsuan , Hsu Hong-Yuan , Chang Mei-Hwei
Published: August 2020

Background

We investigated the relationships among the percentage of hepatitis B virus (HBV) mutations and liver fibrosis after hepatitis B e antigen (HBeAg) seroconversion.

Methods

We quantified the percentage of HBV mutants by pyrosequencing using serum samples obtained at inflammatory phase and after HBeAg seroconversion in 160 initially HBeAg-positive chronic HBV-infected patients. The relationships between antiviral agents, percentages of HBV mutations, and liver stiffness measurements (LSMs) were analyzed.

Results

We demonstrated that the percentages of A1762T/G1764A mutation are significantly higher in subjects with an LSM >7 kPa than in those with an LSM ≤7 kPa after HBeAg seroconversion. Hepatitis B e antigen seroconversion age is positively correlated with the percentages of A1762T/G1764A mutation at inflammatory phase before HBeAg seroconversion. Subjects who underwent interferon, entecavir, or tenofovir disoproxil fumarate therapy before HBeAg seroconversion possessed a lower percentage of A1762T/G1764A mutation after HBeAg seroconversion. The percentage of A1762T/G1764A ≥20% after HBeAg seroconversion was predictive of an LSM >7 kPa (hazard ratio = 6.37, P = .001). The presence of A1762T/G1764A led to downregulated messenger ribonucleic acid and protein levels of programmed-death ligand-1 (PD-L1) in hepatocytes.

Conclusions

The percentage of A1762T/G1764A mutations after HBeAg seroconversion was associated with liver fibrosis. The A1762T/G1764A mutation may evoke hepatic inflammation by suppressing PD-L1 in hepatocytes.
A Review of the Control of Aedes aegypti (Diptera: Culicidae) in the Continental United States
Authors: McGregor Bethany L , Connelly C Roxanne
Published: August 2020

Aedes aegypti (L) is an anthropophilic mosquito involved in the transmission of a variety of viral pathogens worldwide including dengue, chikungunya, yellow fever, and Zika viruses. This species, native to Africa, is well established in the continental U.S. (CONUS) and occasionally contributes to localized outbreaks of viral diseases. In the last seven decades, mosquito control programs in the CONUS have been focused on vectors of eastern equine encephalitis, St. Louis encephalitis, and West Nile viruses, as well as nuisance species. Aedes aegypti receives little control focus except during outbreak periods, which has led to a lack of information on appropriate and effective control options targeting Ae. aegypti in the CONUS. As such, in the event of an Ae. aegypti-borne arboviral outbreak in the CONUS, there are limited evidence-based control recommendations or protocols in place. Autochthonous outbreaks of Ae. aegypti-borne pathogens have occurred recently in the CONUS, including dengue outbreaks in 2010 and 2013, a chikungunya outbreak in 2014, and the 2016 outbreak of Zika virus. The increasing frequency of Ae. aegypti-borne outbreaks necessitates increased attention and research on control of this species to prevent and mitigate future outbreaks. This review consolidates and synthesizes the available literature on control of Ae. aegypti, specifically within the CONUS, focusing on data generated through operational applications as well as field and semifield experiments. The purpose of this review is to identify and highlight areas where additional research is needed. The review covers chemical control and insecticide resistance, biological control, source reduction, trapping, and alternative techniques.