bluuebunny/crossref_metadata_2025 huggingface dataset:
Task-Covariant Representations for Few-Shot Learning on Remote Sensing Images
Authors: Zhang Liyi , Tian Zengguang , Tang Yi , Jiang Zuo
Published: April 2023

In the regression and classification of remotely sensed images through meta-learning, techniques exploit task-invariant information to quickly adapt to new tasks with fewer gradient updates. Despite its usefulness, task-invariant information alone may not effectively capture task-specific knowledge, leading to reduced model performance on new tasks. As a result, the concept of task-covariance has gained significant attention from researchers. We propose task-covariant representations for few-shot Learning on remote sensing images that utilizes capsule networks to effectively represent the covariance relationships among objects. This approach is motivated by the superior ability of capsule networks to capture such relationships. To capture and leverage the covariance relations between tasks, we employ vector capsules and adapt our model parameters based on the newly learned task covariance relations. Our proposed meta-learning algorithm offers a novel approach to effectively address the real task distribution by incorporating both general and specific task information. Based on the experimental results, our proposed meta-learning algorithm shows a significant improvement in both the average accuracy and training efficiency compared to the best model in the experiments. On average, the algorithm increases the accuracy by approximately 4% and improves the training efficiency by approximately 8%.
On the Higher Nash Blow-Up Derivation Lie Algebras of Isolated Hypersurface Singularities
Authors: Asif Muhammad , Al-Kenani Ahmad N. , Hussain Naveed , Binyamin Muhammad Ahsan
Published: April 2023

It is a natural question to ask whether there is any Lie algebra that completely characterize simple singularities? The higher Nash blow-up derivation Lie algebras Lkl(V) associated to isolated hypersurface singularities defined to be the Lie algebra of derivations of the local Artinian algebra Mnl(V):=Ol/⟨F,Jn⟩, i.e., Lkl(V)=Der(Mnl(V)). In this paper, we construct a new conjecture for the complete characterization of simple hypersurface singularities using the Lie algebras Lkl(V) under certain condition and prove it true for Lkl(V) when k,l=2.
Rugulopteryx-Derived Spatane, Secospatane, Prenylcubebane and Prenylkelsoane Diterpenoids as Inhibitors of Nitric Oxide Production
Authors: Cuevas Belén , Arroba Ana I. , de los Reyes Carolina , Zubía Eva
Published: April 2023

This study aimed to evaluate the anti-inflammatory potential of the different classes of diterpenoids produced by algae of the genus Rugulopteryx. First, sixteen diterpenoids (1–16), including spatane, secospatane, prenylcubebane, and prenylkelsoane metabolites, were isolated from the extract of the alga Rugulopteryx okamurae collected at the southwestern Spanish coasts. Eight of the isolated diterpenoids are new compounds whose structures were determined by spectroscopic means: the spatanes okaspatols A-D (1–4); the secospatane rugukamural D (8); the prenylcubebanes okacubols A (13) and B (14); and okamurol A (16), which exhibits an unusual diterpenoid skeleton featuring a kelsoane-type tricyclic nucleus. Second, anti-inflammatory assays were performed on microglial cells Bv.2 and macrophage cells RAW 264.7. Compounds 1, 3, 6, 12, and 16 caused significant inhibition of the NO overproduction induced by LPS in Bv.2 cells, and compounds 3, 5, 12, 14, and 16 significantly decreased levels of NO in LPS-stimulated RAW 264.7 cells. The most active compound was okaspatol C (3), which completely suppressed the effects of LPS stimulation, both in Bv.2 and in RAW 264.7 cells.
Clinical Characterization and Predictive Factors for Progression in a Cohort of Patients with Interstitial Lung Disease and Features of Autoimmunity: The Need for a Revision of IPAF Classification Criteria
Authors: Bozzao Francesco , Tomietto Paola , Baratella Elisa , Kodric Metka , Cifaldi Rossella , Della Porta Rossana , Prearo Ilaria , Pirronello Silvia Maria Grazia , Confalonieri Paola , Ruaro Barbara , Fischetti Fabio , Fabris Bruno
Published: April 2023

Background and Objectives: The “interstitial pneumonia with autoimmune features” (IPAF) criteria have been criticized because of the exclusion of usual interstitial pneumonia (UIP) patients with a single clinical or serological feature. To classify these patients, the term UIPAF was proposed. This study aims to describe clinical characteristics and predictive factors for progression of a cohort of interstitial lung disease (ILD) patients with at least one feature of autoimmunity, applying criteria for IPAF, specific connective tissue diseases (CTD), and a definition of UIPAF when possible. Methods: We retrospectively evaluated data on 133 consecutive patients with ILD at onset associated with at least one feature of autoimmunity, referred by pulmonologists to rheumatologists from March 2009 to March 2020. Patients received 33 (16.5–69.5) months of follow-up. Results: Among the 101 ILD patients included, 37 were diagnosed with IPAF, 53 with ILD-onset CTD, and 11 with UIPAF. IPAF patients had a lower prevalence of UIP pattern compared to CTD-ILD and UIPAF patients (10.8% vs. 32.1% vs. 100%, p < 0.01). During the follow-up, 4 IPAF (10.8%) and 2 UIPAF (18.2%) patients evolved into CTD-ILD. IPAF patients presented features not included in IPAF criteria, such as sicca syndrome (8.1%), and were more frequently affected by systemic hypertension (p < 0.01). Over one year, ILD progression (greater extent of fibrosis on HRCT and/or decline in PFTs) was less frequent in the IPAF group compared to CTD-ILD and UIPAF (32.3% vs. 58.8% vs. 72.7, p = 0.02). A UIP pattern and an IPAF predicted a faster (OR: 3.80, p = 0.01) and a slower (OR: 0.28, p = 0.02) ILD progression, respectively. Conclusions: IPAF criteria help identify patients who might develop a CTD-ILD, even though a single clinical or serological feature is respected. Future revisions of IPAF criteria should include sicca syndrome and separate UIP-pattern into a different definition (UIPAF), given its association with a different prognosis, independently from ILD classification.
Fabrication and Evaluation of Filtration Membranes from Industrial Polymer Waste
Authors: Bano Saleheen , Pednekar Mukesh , Rameshkumar Saranya , Borah Dipu , Morris Michael A. , Padamati Ramesh Babu , Cronly Niamh
Published: April 2023

Polyvinylidene fluoride (PVDF) polymers are known for their diverse range of industrial applications and are considered important raw materials for membrane manufacturing. In view of circularity and resource efficiency, the present work mainly deals with the reusability of waste polymer ‘gels’ produced during the manufacturing of PVDF membranes. Herein, solidified PVDF gels were first prepared from polymer solutions as model waste gels, which were then subsequently used to prepare membranes via the phase inversion process. The structural analysis of fabricated membranes confirmed the retention of molecular integrity even after reprocessing, whereas the morphological analysis showed a symmetric bi-continuous porous structure. The filtration performance of membranes fabricated from waste gels was studied in a crossflow assembly. The results demonstrate the feasibility of gel-derived membranes as potential microfiltration membranes exhibiting a pure water flux of 478 LMH with a mean pore size of ~0.2 µm. To further evaluate industrial applicability, the performance of the membranes was tested in the clarification of industrial wastewater, and the membranes showed good recyclability with about 52% flux recovery. The performance of gel-derived membranes thus demonstrates the recycling of waste polymer gels for improving the sustainability of membrane fabrication processes.
Effect of Coiling Temperature on Microstructure and Properties of Titanium Strengthened Weathering Building Steel
Authors: Li Zhengrong , Lv Zhenhu , Wang Chuangwei , Liu Lei , Cui Kaiyu , Zhao Zhengzhi
Published: April 2023

For weathering steel used in building, it is necessary not only to ensure weather resistance, but also to improve the strength and yield ratio. This study investigates the strengthening effect of Ti microalloying on the tested steel by conducting continuous cooling transformation tests of undercooled austenite and comparative tests of microstructure and performance at different coiling temperatures, with 0.07 wt.% Ti added to the weathering building test steel. The results show that, with an increase in cooling rate (0.1~50 °C/s), the room temperature microstructure of different cooling rates gradually transitions as follows: F + P, F + P + B, F + B and B + M; in addition, the hardness increases. Polygonal ferrite and pearlite were obtained by coiling at 650 °C; quasi-polygonal ferrite, acicular ferrite, pearlite and a small amount of bainite were obtained by coiling at 600 °C; and bainite was obtained by coiling at 550 °C. With a decrease in coiling temperature, the strength of the test steel increased, the yield ratio increased, the elongation after fracture decreased and the elongation at the yield point decreased. Compared with those observed at 650 °C, the nano precipitation phases observed in the sample at 600 °C were smaller in size, higher in number and higher in dislocation density. The combined action of second-phase precipitation strengthening and dislocation strengthening increased the strength and yield ratio of the test steel. This study will be helpful in guiding the improvement of strength grades for weathering steel used in building and industrial production.
Multinuclear Magnetic Resonance Spectroscopy at Ultra-High-Field: Assessing Human Cerebral Metabolism in Healthy and Diseased States
Authors: Veeraiah Pandichelvam , Jansen Jacobus F. A.
Published: April 2023

The brain is a highly energetic organ. Although the brain can consume metabolic substrates, such as lactate, glycogen, and ketone bodies, the energy metabolism in a healthy adult brain mainly relies on glucose provided via blood. The cerebral metabolism of glucose produces energy and a wide variety of intermediate metabolites. Since cerebral metabolic alterations have been repeatedly implicated in several brain disorders, understanding changes in metabolite levels and corresponding cell-specific neurotransmitter fluxes through different substrate utilization may highlight the underlying mechanisms that can be exploited to diagnose or treat various brain disorders. Magnetic resonance spectroscopy (MRS) is a noninvasive tool to measure tissue metabolism in vivo. 1H-MRS is widely applied in research at clinical field strengths (≤3T) to measure mostly high abundant metabolites. In addition, X-nuclei MRS including, 13C, 2H, 17O, and 31P, are also very promising. Exploiting the higher sensitivity at ultra-high-field (>4T; UHF) strengths enables obtaining unique insights into different aspects of the substrate metabolism towards measuring cell-specific metabolic fluxes in vivo. This review provides an overview about the potential role of multinuclear MRS (1H, 13C, 2H, 17O, and 31P) at UHF to assess the cerebral metabolism and the metabolic insights obtained by applying these techniques in both healthy and diseased states.
Design of Hybrid Beamforming System Based on Practical Circuit Parameter of 6-Bit Millimeter-Wave Phase Shifters
Authors: Alqaisei Mohammed A. , Sheta Abdel-Fattah A. , Elshafiey Ibrahim , Altamimi Majid
Published: April 2023

This paper addresses the design of a hybrid beamforming system considering the circuit parameter of six-bit millimeter-wave phase shifters based on the process design kit. The phase shifter design adopts 45 nm CMOS silicon on insulator (SOI) technology at 28-GHz. Various circuit topologies are utilized, and in particular, a design is presented based on switched LC components, connected in a cascode manner. The phase shifter configuration is connected in a cascading manner to get the 6-bit phase controls. Six different phase shifters are obtained, which are 180°, 90°, 45°, 22.5°, 11.25°, and 5.6°, with a minimum number of LC components. The circuit parameters of the designed phase shifters are then incorporated in a simulation model of hybrid beamforming for a multiuser MIMO system. The number of OFDM data symbols used in the simulation is ten for eight users, 16 QAM modulation schemes, −25 dB SNR, 120 simulation runs, and around 170 h runtime. Simulation results are obtained considering four and eight users, assuming accurate technology-based models of RFIC components of the phase shifter as well as ideal phase shifter parameters. The results indicate that the performance of the multiuser MIMO system is affected by the accuracy level of the phase shifter RF component models. The outcomes also reveal the performance tradeoff based on user data streams and the number of BS antennas. By optimizing the amount of parallel data streams per user, higher data transmission rates are achieved, while maintaining acceptable error vector magnitude (EVM) values. In addition, stochastic analysis is conducted to investigate the distribution of the RMS EVM. The outcomes show that the best fitting of RMS EVM distribution of the actual and ideal phase shifters agreed with the log-logistic and logistic distributions, respectively. The obtained (mean, variance) values of the actual phase shifters based on accurate library models are (46.997, 481.36), and for ideal components the values are (36.47, 10.44).
Improving the Detection of Epidemic Clones in Candida parapsilosis Outbreaks by Combining MALDI-TOF Mass Spectrometry and Deep Learning Approaches
Authors: Mohammad Noshine , Normand Anne-Cécile , Nabet Cécile , Godmer Alexandre , Brossas Jean-Yves , Blaize Marion , Bonnal Christine , Fekkar Arnaud , Imbert Sébastien , Tannier Xavier , Piarroux Renaud
Published: April 2023

Identifying fungal clones propagated during outbreaks in hospital settings is a problem that increasingly confronts biologists. Current tools based on DNA sequencing or microsatellite analysis require specific manipulations that are difficult to implement in the context of routine diagnosis. Using deep learning to classify the mass spectra obtained during the routine identification of fungi by MALDI-TOF mass spectrometry could be of interest to differentiate isolates belonging to epidemic clones from others. As part of the management of a nosocomial outbreak due to Candida parapsilosis in two Parisian hospitals, we studied the impact of the preparation of the spectra on the performance of a deep neural network. Our purpose was to differentiate 39 otherwise fluconazole-resistant isolates belonging to a clonal subset from 56 other isolates, most of which were fluconazole-susceptible, collected during the same period and not belonging to the clonal subset. Our study carried out on spectra obtained on four different machines from isolates cultured for 24 or 48 h on three different culture media showed that each of these parameters had a significant impact on the performance of the classifier. In particular, using different culture times between learning and testing steps could lead to a collapse in the accuracy of the predictions. On the other hand, including spectra obtained after 24 and 48 h of growth during the learning step restored the good results. Finally, we showed that the deleterious effect of the device variability used for learning and testing could be largely improved by including a spectra alignment step during preprocessing before submitting them to the neural network. Taken together, these experiments show the great potential of deep learning models to identify spectra of specific clones, providing that crucial parameters are controlled during both culture and preparation steps before submitting spectra to a classifier.
Brine–Melts and Fluids of the Fe-F-P-(Ba)-(Sr)-REE Central Asian Carbonatite Province (Southern Siberia and Mongolia): The Petrogenetic Aspects
Authors: Prokopyev Ilya , Doroshkevich Anna , Redina Anna
Published: April 2023

The carbonatite complexes of the Central Asian carbonatite province comprise the Siberian carbonatites of the Western Transbaikalia and the Central Tuva regions, as well as those from the Mushugai-Khudag complex in Southern Mongolia. They are confined to Late Mesozoic rift structures and have endured considerable tectono-magmatic processes caused by intense plume activity, which also accompanied their formation. A systematic study of melt and fluid inclusions revealed that these carbonatites formed as a result of immiscibility processes in silicate–carbonate (salt) melts, as well as fractional crystallization. Alkaline–carbonatite rocks crystallized in the presence of brine–melts with different compositions, i.e., alkaline–fluorine, carbonate, sulfate, phosphate, and chloride. These melts are responsible for mineralization during the orthomagmatic stage and the primary phase of Fe-F-P-(Ba)-(Sr)-REE ore formation at temperature ranges of 850–830 °C, 650–610 °C, and 560–440 °C and pressures between 290 and 350 MPa. At a later stage, the brine–melts evolved into saline hydrothermal fluids, which are considered to be the source of the second stage of F-(Ba)-(Sr)-REE ore mineralization. The saline crystal–fluid inclusions consist mainly of fluorine–sulfate–carbonate–chloride and bicarbonate–chloride compositions, with temperatures of approximately 480–250 °C and pressures below 250 MPa. The shift from melt to fluid in carbonatite complexes could occur more frequently in nature than previously believed and could also apply to other F-REE carbonatite complexes that are linked to rifting and plume activity in mountain-building zones.
Reconstructing the Tectonic History of the Arabian–Nubian Shield in Sinai: Low-Temperature Thermochronology Implications on Wadi Agar Area
Authors: Mansour Sherif , Hasebe Noriko , Abdelrahman Kamal , Fnais Mohammed S. , Tamura Akihiro
Published: April 2023

The Arabian–Nubian Shield envelops the entire regional tectonic history from its formation during the Ediacaran to the Red Sea/Gulf of Suez rifting in the Oligocene–Miocene. The occurrence and extent of the expected successive tectonic events on Sinai basement rocks remain uncertain. Integration of thermochronological techniques with time–temperature modelling has proven to be a powerful tool for thermal-tectonic history reconstruction. Therefore, we collected representative samples from the Arabian–Nubian Shield basement rocks of the Wadi Agar area at the eastern flank of the Suez rift. Zircon fission-track data show two cooling age possibilities of Ediacaran and Devonian ages. Meanwhile, apatite fission-track data represent three cooling age spans of Carboniferous, Triassic, and Cretaceous. The integration of these data with the modelled time–temperature histories reveals four different cooling events synchronous with the regional events; (1) the Neoproterozoic post-accretion erosional event that causes near-surface rock uplift, (2) the Devonian–Carboniferous Hercynian tectonic event which affected the region with rocks exhumation of ca. 4.2 ± 1.4 km, (3) the Triassic Gondwana breakup initiation, and (4) the Oligocene–Miocene Gulf of Suez rifting which caused flanks uplift in the studied region of ca. 1.2 ± 0.4 km. The Gulf of Suez is a passive rift with a dominant mechanical component that is divided into two differently exhumed northern and southern segments, where an additional far-field thermal overprint was restricted to the southern segment.
The Depositional Environment of the Lacustrine Source Rocks in the Eocene Middle Number of the Liushagang Formation of the Weixinan Sag, Beibuwan Basin, China: Implications from Organic Geochemical Analyses
Authors: Yang Xiaoyong , Lv Xiaoxia , Huang Yahao , He Yunlong , Yang Rui , Wang Ruyue , Peng Peng
Published: April 2023

The Eocene middle number of the Liushagang Formation (LS2) of the Weixinan Sag, Beibuwan Basin, characterized by a thick succession of excellent quality source rocks, is composed of lacustrine organic-rich shales, mudstones, and shales (mudstones/shales). However, the complex and specific depositional environment in the source rocks of LS2 raise questions about the mainly controlling factors of lacustrine organic matter (OM) accumulation. In this study, total organic carbon (TOC) contents, Rock-Eval pyrolysis, as well as biomarker data are used to investigate the nature of the depositional environment and the enrichment mechanism of OM in the source rocks of LS2. The values of Tmax, CPI, C29 steranes αββ/(ααα+αββ), and the 22S/(22S+22R) ratios of the 17α, 21β(H)-C31 hopane together confirm that the OM in the source rocks of LS2 is immature to of low maturity, which suggests that the nature of biomarkers may not be affected by thermal maturity. The hydrocarbon potential was higher in the organic-rich shales (with a mean of 20.99 mg/g) than in the mudstones/shales (with a mean of 7.10 mg/g). The OM in organic-rich shales is type I and II kerogen and that in mudstones/shales is type II kerogen. The C27/C29 regular steranes ratios and 4-methylsterane indices (4MSI) further confirmed the difference in the source of OM between organic-rich shales and mudstones/shales; that is, that the OM of organic-rich shales is mainly derived from the lake algae and aquatic macrophytes and the OM of mudstones/shales is mainly from the higher plants. The values of the gammacerane index and ratios of C21/C23 TT and C24 Tet/C26 TT all indicate that the source rocks from LS2 are deposited in freshwater to a low salinity water column. Moreover, a cross-plot of C21–22/C27–29 sterane versus dia/reg C27 sterane ratios and Pr/Ph ratios suggests that the source rocks from LS2 are recorded to have sub-oxic to oxic conditions. Based on those analyses, two dynamical formation models were proposed: a high-productivity and oxic-suboxic dynamical formation model (Model A) and a low-productivity and oxic-suboxic dynamical formation model (Model B).
Silver-Promoted Radical Cascade Aryldifluoromethylation/Cyclization of 2-Allyloxybenzaldehydes for the Synthesis of 3-Aryldifluoromethyl-Containing Chroman-4-one Derivatives
Authors: Sun Qianqian , Li Hongxiao , Chen Xingyu , Hao Jian , Deng Hongmei , Jiang Haizhen
Published: April 2023

A convenient silver-promoted radical cascade aryldifluoromethylation/cyclization of 2-allyloxybenzaldehydes has been developed. Experimental studies disclosed that the addition of aryldifluoromethyl radicals in situ produced from easily accessible gem-difluoroarylacetic acids to unactivated double bonds in 2-allyloxybenzaldehyde was an effective route to access a series of 3-aryldifluoromethyl-containing chroman-4-one derivatives in moderate to good yields under mild reaction conditions.
DFT Study of Adsorption Behavior of Nitro Species on Carbon-Doped Boron Nitride Nanoribbons for Toxic Gas Sensing
Authors: Villanueva-Mejia Francisco , Guevara-Martínez Santiago José , Arroyo-Albiter Manuel , Alvarado-Flores José Juan , Zamudio-Ojeda Adalberto
Published: April 2023

The modifications of the electronic properties on carbon-doped boron nitride nanoribbons (BNNRs) as a response to the adsorption of different nitro species were investigated in the framework of the density functional theory within the generalized gradient approximation. Calculations were performed using the SIESTA code. We found that the main response involved tuning the original magnetic behavior to a non-magnetic system when the molecule was chemisorbed on the carbon-doped BNNR. It was also revealed that some species could be dissociated through the adsorption process. Furthermore, the nitro species preferred to interact over nanosurfaces where dopants substituted the B sublattice of the carbon-doped BNNRs. Most importantly, the switch on the magnetic behavior offers the opportunity to apply these systems to fit novel technological applications.
Enhanced Electrochemical Performance of Metallic CoS-Based Supercapacitor by Cathodic Exfoliation
Authors: Tian Ye , Ma Yuxin , Sun Ruijin , Zhang Weichao , Liu Haikun , Liu Hao , Liao Libing
Published: April 2023

Two-dimensional nanomaterials hold great promise as electrode materials for the construction of excellent electrochemical energy storage and transformation apparatuses. In the study, metallic layered cobalt sulfide was, firstly, applied to the area of energy storage as a supercapacitor electrode. By a facile and scalable method for cathodic electrochemical exfoliation, metallic layered cobalt sulfide bulk can be exfoliated into high-quality and few-layered nanosheets with size distributions in the micrometer scale range and thickness in the order of several nanometers. With a two-dimensional thin sheet structure of metallic cobalt sulfide nanosheets, not only was a larger active surface area created, but also, the insertion/extraction of ions in the procedure of charge and discharge were enhanced. The exfoliated cobalt sulfide was applied as a supercapacitor electrode with obvious improvement compared with the original sample, and the specific capacitance increased from 307 F∙g−1 to 450 F∙g−1 at the current density of 1 A∙g−1. The capacitance retention rate of exfoliated cobalt sulfide enlarged to 84.7% from the original 81.9% of unexfoliated samples while the current density multiplied by 5 times. Moreover, a button-type asymmetric supercapacitor assembled using exfoliated cobalt sulfide as the positive electrode exhibits a maximum specific energy of 9.4 Wh∙kg−1 at the specific power of 1520 W∙kg−1.
Advances in WO3-Based Supercapacitors: State-of-the-Art Research and Future Perspectives
Authors: Mineo Giacometta , Bruno Elena , Mirabella Salvo
Published: April 2023

Electrochemical energy storage devices are one of the main protagonists in the ongoing technological advances in the energy field, whereby the development of efficient, sustainable, and durable storage systems aroused a great interest in the scientific community. Batteries, electrical double layer capacitors (EDLC), and pseudocapacitors are characterized in depth in the literature as the most powerful energy storage devices for practical applications. Pseudocapacitors bridge the gap between batteries and EDLCs, thus supplying both high energy and power densities, and transition metal oxide (TMO)-based nanostructures are used for their realization. Among them, WO3 nanostructures inspired the scientific community, thanks to WO3’s excellent electrochemical stability, low cost, and abundance in nature. This review analyzes the morphological and electrochemical properties of WO3 nanostructures and their most used synthesis techniques. Moreover, a brief description of the electrochemical characterization methods of electrodes for energy storage, such as Cyclic Voltammetry (CV), Galvanostatic Charge–Discharge (GCD), and Electrochemical Impedance Spectroscopy (EIS) are reported, to better understand the recent advances in WO3-based nanostructures, such as pore WO3 nanostructures, WO3/carbon nanocomposites, and metal-doped WO3 nanostructure-based electrodes for pseudocapacitor applications. This analysis is reported in terms of specific capacitance calculated as a function of current density and scan rate. Then we move to the recent progress made for the design and fabrication of WO3-based symmetric and asymmetric supercapacitors (SSCs and ASCs), thus studying a comparative Ragone plot of the state-of-the-art research.
Effects of Simulated In Vitro Digestion on the Structural Characteristics, Inhibitory Activity on α-Glucosidase, and Fermentation Behaviours of a Polysaccharide from Anemarrhena asphodeloides Bunge
Authors: Chen Juncheng , Lan Meijuan , Zhang Xia , Jiao Wenjuan , Chen Zhiyi , Li Lin , Li Bing
Published: April 2023

The purpose of this study is to investigate the effects of the simulated saliva–gastrointestinal digestion of AABP-2B on its structural features, inhibitory α-glucosidase activity, and human gut microbiota. The salivary–gastrointestinal digestion results show that there is no significant change in the molecular weight of AABP-2B, and no free monosaccharides are released. This indicates that, under a simulated digestive condition, AABP-2B is not degraded and can be further utilized by gut microbiota. AABP-2B still possessed good inhibitory activity on α-glucosidase after salivary–gastrointestinal digestion, which may be attributed to the largely unchanged structural characteristics of AABP-2B after simulated digestion. Furthermore, in vitro fecal fermentation with AABP-2B after salivary–gastrointestinal digestion showed that AABP-2B modulated the gut microbiota structure and increased the relative proportions of Prevotella, Faecalibacterium, and Megasphaera. AABP-2B can also modify the intestinal flora composition by inhibiting pathogen growth. Moreover, the AABP-2B group resulted in a significant increase in short-chain fatty acid (SCFAs) content during fermentation. These findings demonstrate that AABP-2B can be used as a prebiotic or functional food to promote gut health.
Effectiveness and Coverage of Severe Acute Malnutrition Treatment with a Simplified Protocol in a Humanitarian Context in Diffa, Niger
Authors: Charle-Cuéllar Pilar , Lopez-Ejeda Noemi , Aziz Gado Abdoul , Dougnon Abdias Ogobara , Sanoussi Atté , Ousmane Nassirou , Hamidou Lazoumar Ramatoulaye , Sánchez-Martínez Luis Javier , Toure Fanta , Vargas Antonio , Guerrero Saul
Published: April 2023

Background: the aim of this study is to evaluate the effectiveness and coverage of a simplified protocol that is implemented in health centers (HCs) and health posts (HPs) for children who are suffering from severe acute malnutrition (SAM) in the humanitarian context of Diffa. Methods: We conducted a non-randomized community-controlled trial. The control group received outpatient treatment for SAM, without medical complications, at HCs and HPs with the standard protocol of community management of acute malnutrition (CMAM). Meanwhile, with respect to the intervention group, the children with SAM received treatment at the HCs and HPs through a simplified protocol wherein the mid-upper arm circumference (MUAC) and the presence of edema were used as the admission criteria, and the children with SAM were administered doses of fixed ready-to-use therapeutic food (RUTF). Results: A total of 508 children, who were all under 5 years and had SAM, were admitted into the study. The cured proportion was 87.4% in the control group versus 96.6% in the intervention group (p value = 0.001). There was no difference between the groups in the length of stay, which was 35 days, but the intervention group used a lower quantity of RUTF—70 sachets versus 90 sachets, per child cured. Coverage increases were observed in both groups. Discussion: the simplified protocol used at the HCs and HPs did not result in worse recovery and resulted in fewer discharge errors compared to the standard protocol.
Handgrip Strength Test and Bioelectrical Impedance Analysis in SARS-CoV-2 Patients Admitted to Sub-Intensive Unit
Authors: Zotti Sonia , Luci Isabella , Finamore Panaiotis , Travaglino Francesco , Pedone Claudio , Antonelli Incalzi Raffaele
Published: April 2023

Hospitalized patients with respiratory failure due to SARS-CoV-2 pneumonia are at increased risk of malnutrition and related mortality. The predictive value of the Mini-Nutritional Assessment short form (MNA-sf®), hand-grip strength (HGS), and bioelectrical impedance analysis (BIA) was determined with respect to in-hospital mortality or endotracheal intubation. The study included 101 patients admitted to a sub-intensive care unit from November 2021 to April 2022. The discriminative capacity of MNA-sf, HGS, and body composition parameters (skeletal mass index and phase angle) was assessed computing the area under the receiver operating characteristic curves (AUC). Analyses were stratified by age groups (<70/70+ years). The MNA-sf alone or in combination with HGS or BIA was not able to reliably predict our outcome. In younger participants, HGS showed a sensitivity of 0.87 and a specificity of 0.54 (AUC: 0.77). In older participants, phase angle (AUC: 0.72) was the best predictor and MNA-sf in combination with HGS had an AUC of 0.66. In our sample, MNA- sf alone, or in combination with HGS and BIA was not useful to predict our outcome in patients with COVID-19 pneumonia. Phase angle and HGS may be useful tools to predict worse outcomes in older and younger patients, respectively.
Trends in Diet Quality and Increasing Inadequacies of Micronutrients Vitamin C, Vitamin B12, Iron and Potassium in US Type 2 Diabetic Adults
Authors: Sun Hongbing , Weaver Connie M.
Published: April 2023

BACKGROUND: Prevalence of diabetes was high and rose significantly in the US between 1999 and 2018. A healthy dietary pattern that provides micronutrient adequacy is one of the most important lifestyle choices for battling the progress of diabetes. Yet, the patterns and trends of diet quality of the US type 2 diabetes are understudied. OBJECTIVES: We aim to examine the patterns and trends of diet quality and major food sources of macronutrients of US type 2 diabetic adults. METHODS: The 24 h dietary recalls of 7789 type 2 diabetic adults, comprising 94.3% of total adults with diabetes from the US National Health and Nutrition Examination Survey cycles (1999–2018), were analyzed. Diet quality was measured by the total Healthy Eating Index (HEI)-2015 scores and 13 individual components. Trends of usual intakes of vitamin C (VC), vitamin B12 (VB12), iron, and potassium and supplements from two 24 h recalls were also examined for type 2 diabetic population. RESULTS: Diet quality of type 2 diabetic adults worsened between 1999 and 2018 while that of US adults of general population improved based on the total HEI 2015 scores. For people with type 2 diabetes, consumption of saturated fat and added sugar increased while consumption of vegetables and fruits declined significantly, although consumption of refined grain declined and consumption of seafood and plant protein increased significantly. In addition, usual intakes of micronutrients VC, VB12, iron, and potassium from food sources declined significantly during this period. CONCLUSIONS: Diet quality generally worsened for US type 2 diabetic adults between 1999 and 2018. Declining consumptions of fruits, vegetables, and non-poultry meat may have contributed to the increasing inadequacies of VC, VB12, iron, and potassium in the US type 2 diabetic adults.
Effects of Gut Microbiome Modulation on Reducing Adverse Health Outcomes among Elderly and Diabetes Patients during the COVID-19 Pandemic: A Randomised, Double-Blind, Placebo-Controlled Trial (IMPACT Study)
Authors: Wong Martin C. S. , Zhang Lin , Ching Jessica Y. L. , Mak Joyce W. Y. , Huang Junjie , Wang Shilan , Mok Chris K. P. , Wong Angie , Chiu Oi-Lee , Fung Yee-Ting , Cheong Pui-Kuan , Tun Hein-Min , Ng Siew C. , Chan Francis K. L.
Published: April 2023

Gut microbiota is believed to be a major determinant of health outcomes. We hypothesised that a novel oral microbiome formula (SIM01) can reduce the risk of adverse health outcomes in at-risk subjects during the coronavirus disease 2019 (COVID-19) pandemic. In this single-centre, double-blind, randomised, placebo-controlled trial, we recruited subjects aged ≥65 years or with type two diabetes mellitus. Eligible subjects were randomised in a 1:1 ratio to receive three months of SIM01 or placebo (vitamin C) within one week of the first COVID-19 vaccine dose. Both the researchers and participants were blinded to the groups allocated. The rate of adverse health outcomes was significantly lower in the SIM01 group than the placebo at one month (6 [2.9%] vs. 25 [12.6], p < 0.001) and three months (0 vs. 5 [3.1%], p = 0.025). At three months, more subjects who received SIM01 than the placebo reported better sleep quality (53 [41.4%] vs. 22 [19.3%], p < 0.001), improved skin condition (18 [14.1%] vs. 8 [7.0%], p = 0.043), and better mood (27 [21.2%] vs. 13 [11.4%], p = 0.043). Subjects who received SIM01 showed a significant increase in beneficial Bifidobacteria and butyrate-producing bacteria in faecal samples and strengthened the microbial ecology network. SIM01 reduced adverse health outcomes and restored gut dysbiosis in elderly and diabetes patients during the COVID-19 pandemic.
Comment on Sifakaki et al. Orthorexia Nervosa Practices in Rheumatoid Arthritis: The DORA Study. Nutrients 2023, 15, 713
Authors: Meule Adrian
Published: April 2023

In a recently published article, Sifakaki and colleagues [...]
Natural and Engineered Resistance Mechanisms in Plants against Phytoviruses
Authors: Majumdar Anik , Sharma Abhishek , Belludi Rakesh
Published: April 2023

Plant viruses, as obligate intracellular parasites, rely exclusively on host machinery to complete their life cycle. Whether a virus is pathogenic or not depends on the balance between the mechanisms used by both plants and viruses during the intense encounter. Antiviral defence mechanisms in plants can be of two types, i.e., natural resistance and engineered resistance. Innate immunity, RNA silencing, translational repression, autophagy-mediated degradation, and resistance to virus movement are the possible natural defence mechanisms against viruses in plants, whereas engineered resistance includes pathogen-derived resistance along with gene editing technologies. The incorporation of various resistance genes through breeding programmes, along with gene editing tools such as CRISPR/Cas technologies, holds great promise in developing virus-resistant plants. In this review, different resistance mechanisms against viruses in plants along with reported resistance genes in major vegetable crops are discussed.
Characterization and Control of Dendrobium officinale Bud Blight Disease
Authors: Zhang Jinzhao , Sha Haodong , Chen Weiliang , Mao Bizeng
Published: April 2023

Dendrobium officinale is an important traditional Chinese medicine (TCM). A disease causing bud blight in D. officinale appeared in 2021 in Yueqing city, Zhejiang Province, China. In this paper, 127 isolates were obtained from 61 plants. The isolates were grouped into 13 groups based on collected areas and morphological observations. Four loci (ITS, LSU, tub2 and rpb2) of 13 representative isolates were sequenced and the isolates were identified by constructing phylogenetic trees with the multi-locus sequence analysis (MLSA) method. We found the disease to be associated with three strains: Ectophoma multirostrata, Alternaria arborescens and Stagonosporopsis pogostemonis, with isolates frequencies of 71.6%, 21.3% and 7.1%, respectively. All three strains are pathogenic to D. officinale. A. arborescens and S. pogostemonis isolated from D. officinale were reported for the first time. Iprodione (50%), 33.5% oxine-copper and Meitian (containing 75 g/L pydiflumetofen and 125 g/L difenoconazole) were chosen to control the dominant pathogen E. multirostrata, with an EC50 value of 2.10, 1.78 and 0.09 mg/L, respectively. All three fungicides exhibited an effective inhibition of activities to the growth of the dominant pathogen E. multirostrata on potato dextrose agar (PDA) plates, with Meitian showing the strongest inhibitory effect. We further found that Meitian can effectively control D. officinale bud blight disease in pot trial.
Disruption of Proteostasis by Natural Products and Synthetic Compounds That Induce Pervasive Unfolding of Proteins: Therapeutic Implications
Authors: Vilaboa Nuria , Lopez Juan Antonio , de Mesa Marco , Escudero-Duch Clara , Winfield Natalie , Bayford Melanie , Voellmy Richard
Published: April 2023

Exposure of many cancer cells, including multiple myeloma cells, to cytotoxic concentrations of natural products celastrol and withaferin A or synthetic compounds of the IHSF series resulted in denaturation of a luciferase reporter protein. Proteomic analysis of detergent-insoluble extract fractions from HeLa-derived cells revealed that withaferin A, IHSF058 and IHSF115 caused denaturation of 915, 722 and 991 of 5132 detected cellular proteins, respectively, of which 440 were targeted by all three compounds. Western blots showed that important fractions of these proteins, in some cases approaching half of total protein amounts, unfolded. Relatively indiscriminate covalent modification of target proteins was observed; 1178 different proteins were modified by IHSF058. Further illustrating the depth of the induced proteostasis crisis, only 13% of these proteins detectably aggregated, and 79% of the proteins that aggregated were not targets of covalent modification. Numerous proteostasis network components were modified and/or found in aggregates. Proteostasis disruption caused by the study compounds may be more profound than that mediated by proteasome inhibitors. The compounds act by a different mechanism that may be less susceptible to resistance development. Multiple myeloma cells were particularly sensitive to the compounds. Development of an additional proteostasis-disrupting therapy of multiple myeloma is suggested.
Super Carbonate Apatite-miR-497a-5p Complex Is a Promising Therapeutic Option against Inflammatory Bowel Disease
Authors: Tsujimura Naoto , Ogino Takayuki , Hiraki Masayuki , Kai Taisei , Yamamoto Hiroyuki , Hirose Haruka , Yokoyama Yuhki , Sekido Yuki , Hata Tsuyoshi , Miyoshi Norikatsu , Takahashi Hidekazu , Uemura Mamoru , Mizushima Tsunekazu , Doki Yuichiro , Eguchi Hidetoshi , Yamamoto Hirofumi
Published: April 2023

The incidence of inflammatory bowel disease (IBD) is increasing worldwide. It is reported that TGF-β/Smad signal pathway is inactivated in patients with Crohn’s disease by overexpression of Smad 7. With expectation of multiple molecular targeting by microRNAs (miRNAs), we currently attempted to identify certain miRNAs that activate TGF-β/Smad signal pathway and aimed to prove in vivo therapeutic efficacy in mouse model. Through Smad binding element (SBE) reporter assays, we focused on miR-497a-5p. This miRNA is common between mouse and human species and enhanced the activity of TGF-β/Smad signal pathway, decreased Smad 7 and/or increased phosphorylated Smad 3 expression in non-tumor cell line HEK293, colorectal cancer cell line HCT116 and mouse macrophage J774a.1 cells. MiR-497a-5p also suppressed the production of inflammatory cytokines TNF-α, IL-12p40, a subunit of IL-23, and IL-6 when J774a.1 cells were stimulated by lipopolysaccharides (LPS). In a long-term therapeutic model for mouse dextran sodium sulfate (DSS)-induced colitis, systemic delivery of miR-497a-5p load on super carbonate apatite (sCA) nanoparticle as a vehicle restored epithelial structure of the colonic mucosa and suppressed bowel inflammation compared with negative control miRNA treatment. Our data suggest that sCA-miR-497a-5p may potentially have a therapeutic ability against IBD although further investigation is essential.
Precision Oncology by Point-of-Care Therapeutic Drug Monitoring and Dosage Adjustment of Conventional Cytotoxic Chemotherapies: A Perspective
Authors: Briki Myriam , André Pascal , Thoma Yann , Widmer Nicolas , Wagner Anna D. , Decosterd Laurent A. , Buclin Thierry , Guidi Monia , Carrara Sandro
Published: April 2023

Therapeutic drug monitoring (TDM) of conventional cytotoxic chemotherapies is strongly supported yet poorly implemented in daily practice in hospitals. Analytical methods for the quantification of cytotoxic drugs are instead widely presented in the scientific literature, while the use of these therapeutics is expected to keep going for longer. There are two main issues hindering the implementation of TDM: turnaround time, which is incompatible with the dosage profiles of these drugs, and exposure surrogate marker, namely total area under the curve (AUC). Therefore, this perspective article aims to define the adjustment needed from current to efficient TDM practice for cytotoxics, namely point-of-care (POC) TDM. For real-time dose adjustment, which is required for chemotherapies, such POC TDM is only achievable with analytical methods that match the sensitivity and selectivity of current methods, such as chromatography, as well as model-informed precision dosing platforms to assist the oncologist with dose fine-tuning based on quantification results and targeted intervals.
Double-Loaded Doxorubicin/Resveratrol Polymeric Micelles Providing Low Toxicity on Cardiac Cells and Enhanced Cytotoxicity on Lymphoma Cells
Authors: Radeva Lyubomira , Yordanov Yordan , Spassova Ivanka , Kovacheva Daniela , Tzankova Virginia , Yoncheva Krassimira
Published: April 2023

The anthracycline antibiotic doxorubicin is a well-known antitumour agent, however its cardiotoxicity is a significant obstacle to therapy. The aim of the present study was to improve the safety of doxorubicin through its simultaneous encapsulation with a cardioprotective agent (resveratrol) in Pluronic micelles. The formation and double-loading of the micelles was performed via the film hydration method. Infrared spectroscopy proved the successful incorporation of both drugs. X-ray diffraction analyses revealed that resveratrol was loaded in the core, whereas doxorubicin was included in the shell. The double-loaded micelles were characterised by a small diameter (26 nm) and narrow size distribution, which is beneficial for enhanced permeability and retention effects. The in vitro dissolution tests showed that the release of doxorubicin depended on the pH of the medium and was faster than that of resveratrol. In vitro studies on cardioblasts showed the opportunity to reduce the cytotoxicity of doxorubicin through the presence of resveratrol in double-loaded micelles. Higher cardioprotection was observed when the cells were treated with the double-loaded micelles compared with referent solutions with equal concentrations of both drugs. In parallel, treatments of L5178 lymphoma cells with the double-loaded micelles revealed that the cytotoxic effect of doxorubicin was enhanced. Thus, the study demonstrated that the simultaneous delivery of doxorubicin and resveratrol via the micellar system enabled the cytotoxicity of doxorubicin in lymphoma cells and lowered its cardiotoxicity in cardiac cells.
Experimental Elucidation of Templated Crystallization and Secondary Processing of Peptides
Authors: Verma Vivek , Bade Isha , Karde Vikram , Heng Jerry Y. Y.
Published: April 2023

The crystallization of peptides offers a sustainable and inexpensive alternative to the purification process. In this study, diglycine was crystallised in porous silica, showing the porous templates’ positive yet discriminating effect. The diglycine induction time was reduced by five-fold and three-fold upon crystallising in the presence of silica with pore sizes of 6 nm and 10 nm, respectively. The diglycine induction time had a direct relationship with the silica pore size. The stable form (α-form) of diglycine was crystallised in the presence of porous silica, with the diglycine crystals obtained associated with the silica particles. Further, we studied the mechanical properties of diglycine tablets for their tabletability, compactability, and compressibility. The mechanical properties of the diglycine tablets were similar to those of pure MCC, even with the presence of diglycine crystals in the tablets. The diffusion studies of the tablets using the dialysis membrane presented an extended release of diglycine through the dialysis membrane, confirming that the peptide crystal can be used for oral formulation. Hence, the crystallization of peptides preserved their mechanical and pharmacological properties. More data on different peptides can help us produce oral formulation peptides faster than usual.