bluuebunny/crossref_metadata_2025 huggingface dataset:
PENINGKATAN PEMBERDAYAAN KELOMPOK WANITA TANI DUSUN WONOREJO KOTA BATU MELALUI PELATIHAN PEMBUATAN PRODUK SUSU PROBIOTIK-SARI BUAH SEBAGAI MINUMAN KESEHATAN
Authors: Diyah Nuzul Wahyuning , Isnaeni Isnaeni , Darmawati Asri , Hardjono Suko
Published: July 2020

Wonorejo village in Batu city is known as a vegetable and fruit producing area. Another popular product of Batu mostly is fresh dairy milk and only a small amount of processed products while the cow milk collected in KUD Batu has reached 16-17 tons per day. The problem was that the target community had no experiences in processing cow milk using probiotic microbes. The purpose was transfering of science and technology about the production of probiotic milk combined with fruit juice. This program was specifically directed to increase the empowerment of Kelompok Wanita Tani "Wonoasri" in Wonorejo, in developing of fruity- probiotic dairy products to increase the diversification of products with higher economic value. The method included workshop and technical assistance, which were carried out in a serie of steps: 1) exploring the knowledge of target by surveys and pre test, 2) lecturing and discussion, 3) demonstrating how to prepare and characterize the probiotic milk products, 4) training the preparation of product, 5) product characterization and packaging assistance, 6) evaluation. The results of this program were the target community gained increase in knowledge and experience, and they could produce fruity probiotic milk. In addition, we expected that they would increase their understanding and enthusiasm to appreciate the potency of their territory in order to improve the nation's competitiveness. abstrakDesa Wonorejo di kota Batu dikenal sebagai daerah penghasil sayur dan buah. Produk populer lain dari Batu adalah susu segar dan sejumlah kecil produk olahan.  Susu sapi dikumpulkan di KUD Batu. Masalahnya adalah bahwa komunitas target tidak memiliki pengalaman dalam memproses susu sapi menggunakan mikroba probiotik. Tujuannya adalah mentransfer ilmu pengetahuan dan teknologi tentang produksi susu probiotik yang dikombinasikan dengan jus buah. Program ini diarahkan untuk pemberdayaan Kelompok Tani Wanita "Wonoasri" di Wonorejo, dalam mengembangkan produk susu buah-probiotik untuk diversifikasi produk dengan nilai ekonomi lebih tinggi. Metode tersebut termasuk lokakarya dan bantuan teknis, dalam serangkaian langkah: 1) mengeksplorasi pengetahuan target dengan survei dan pra-tes, 2) ceramah dan diskusi, 3) menunjukkan bagaimana mempersiapkan dan mengkarakterisasi produk susu probiotik, 4) pelatihan persiapan produk, 5) karakterisasi produk dan bantuan pengemasan, 6) evaluasi. Hasil program ini adalah komunitas sasaran memperoleh peningkatan dalam pengetahuan dan pengalaman, dan mereka dapat menghasilkan susu probiotik buah. Selain itu, kami berharap bahwa mereka akan meningkatkan pemahaman dan antusiasme mereka untuk menghargai potensi wilayah mereka untuk meningkatkan daya saing bangsa.
Working From Home Phenomenon As an Effort to Prevent COVID-19 Attacks and Its Impacts on Work Productivity
Authors: Mustajab Duta , Bauw Azies , Rasyid Abdul , Irawan Andri , Akbar Muhammad Aldrin , Hamid Muhammad Amin
Published: July 2020

COVID-19 attacks have changed the methods and cultures of work in many organizations, the working from home (WFH) phenomenon as an effort to prevent the spread of COVID-19 in many countries in the world has impacts on employee productivity. This study aims to explore the impacts of working from home on employee productivity with a qualitative approach. The findings explain that working from home has provided advantages and disadvantages both for employee and organization as well as being responsible for the decline in employee productivity. Furthermore, we also find the fact that working from home cannot be generally accepted since many areas of work cannot be carried out from home, although for many employees, working from home has provided a work-life balance. Yet, this is sometimes interrupted by multiple jobs (multitasking) that must be done at home.
Difficulties of Educating People With Socio-Educational Needs
Authors: Simonenko S.M. , Klyuchnikov D.A. , Yunevich M.N. , Suprunov S.E. , Kuprieva I.A.
Published: July 2020

The publication “Problems of introducing inclusive education in the social environment of Ussuriysk city” examines the complex problems and sociocultural processes of society concerning people with special socio-educational needs, people with disabilities and people with physical disabilities. The paper briefly explains the essence of inclusive education and the problems of its introduction into the social environment of small towns, for example, the city of Ussuriysk, when, along with the development of defectological schools and the government order for them, there is a lack of understanding of the need for such education, an indifferent, callous attitude towards disadvantaged physical disabilities to people.
“COVID-19” MEME IN SOCIAL MEDIA: STUDY OF ROLAND BARTHES SEMIOLOGY
Authors: Isnaniah Siti , Tiya Agustina
Published: July 2020

ABSTRACT

The objective of this research is to reveal the meaning of Covid-19 memes in social media using study of Roland Barthes semiology. In addition, this research also explains the social representation in Covid-19 memes. The method of the research is qualitative descriptive using technical analysis of Roland Barthes semiology. The technique of collecting data is based on two resources. The primary data are collections of memes about Covid 19 in some social media, such as Instagram, Facebook, and Web Blog. The secondary data are references supporting the topic of the research. In validity technique, the primary data are collected based on the topic of the discussion. After that, the data are processed descriptively by explaining, and comparing the information gained from many resources. Then, the data are reduced and presented. The result of the study shows that the creators tried to reveal social condition through Covid-19 memes. The social conditions were presented in some types of memes, namely critical memes, parodist memes, and motivational memes. In relation to meaning presentation, some social problems were revealed Covid-19 memes.

Keywords: Meme, Covid-19, Social Media, Roland Barthes Semiology
PERANAN KELUARGA SEKOLAH DAN MASYARAKAT DALAM MENUNJANG PEMBELAJARAN YANG EFEKTIF
Authors: Rochanah Rochanah
Published: July 2020

Education is a shared responsibility between schools families and communities. The purpose of teaching and learning is to create change towards a better state. Learners are expected to transform themselves with reference to subjects who had just obtained. Learning intended to be something that is not known by the students will be known. According to Gagne, changes in behavior as a result of learning can take the form of verbal information, information in the form of verbal mastery of both written and spoken. Intellectual faculties, namely the individual skills in interacting with its environment by using symbols. Cognitive strategies, namely an individual's ability to perform the control and overall management activities. Attitude, namely learning outcomes in the form of an individual's ability to choose the type of action to be performed. Motor skills, ie learning outcomes in the form of skills that is controlled by muscle movement and physical. To support these goals, schools, families and communities must work together synergistically in order to succeed and the realization of effective learning. With the intervention and involvement among the three will certainly be able to support and to support effective learning in school.
The State of Rural Public Health: Enduring Needs in a New Decade
Authors: Leider Jonathon P. , Meit Michael , McCullough J. Mac , Resnick Beth , Dekker Debra , Alfonso Y. Natalia , Bishai David
Published: July 2020

Public health in the rural United States is a complex and underfunded enterprise. While urban–rural disparities have been a focus for researchers and policymakers alike for decades, inequalities continue to grow. Life expectancy at birth is now 1 to 2 years greater between wealthier urban and rural counties, and is as much as 5 years, on average, between wealthy and poor counties.

This article explores the growth in these disparities over the past 40 years, with roots in structural, economic, and social spending differentials that have emerged or persisted over the same time period. Importantly, a focus on place-based disparities recognizes that the rural United States is not a monolith, with important geographic and cultural differences present regionally. We also focus on the challenges the rural governmental public health enterprise faces, the so-called “double disparity” of worse health outcomes and behaviors alongside modest investment in health departments compared with their nonrural peers.

Finally, we offer 5 population-based “prescriptions” for supporting rural public health in the United States. These relate to greater investment and supporting rural advocacy to better address the needs of the rural United States in this new decade.
Cooperative Extension as a Force for Healthy, Rural Communities: Historical Perspectives and Future Directions
Authors: Buys David R. , Rennekamp Roger
Published: July 2020

Cooperative Extension (Extension), part of the land-grant university system, has been engaged in rural communities for more than a century. While the focus of Extension’s efforts has largely centered on agriculture, there is an important thread of work that has similarities to public health.

As Extension settles into its second century, we are working to be even more engaged in efforts that improve the health and well-being of rural communities in particular. Extension faculty and staff are accomplishing this through direct-to-the-population education and through partnerships with more classically oriented public health organizations able to leverage Extension’s networks and positive reputation in communities to engage them and improve their health. A component of these partnerships includes Extension faculty and staff increasingly engaging in policy, systems, and environment work and other initiatives that help ensure longer-term, systemic changes more likely to improve health outcomes.

In short, Extension clearly changed the agricultural system of the United States, and because of its reach into rural communities, it has the capacity to do for health in rural communities in this second century what it did for agriculture in the first century.
Hepatitis A Outbreaks Associated With the Opioid Epidemic in Kentucky Counties, 2017–2018
Authors: DuPre Natalie , Blair Lyndsey , Moyer Sarah , Cook E. Francis , Little Bert , Howard Jeffrey
Published: July 2020

Objectives. To describe county-level socioeconomic profiles associated with Kentucky’s 2017–2018 hepatitis A outbreak that predominately affected communities affected by the opioid epidemic.

Methods. We linked county-level characteristics on socioeconomic and housing variables to counties’ hepatitis A rates. Principal component analysis identified county profiles of poverty, education, disability, income inequality, grandparent responsibility, residential instability, and marital status. We used Poisson regression to estimate adjusted relative risks (RRs) and 95% confidence intervals (CIs).

Results. Counties with scores reflecting an extremely disadvantaged profile (RR = 1.21; 95% CI = 0.99, 1.48) and greater percentage of nonmarried men, residential instability, and income inequality (RR = 1.15; 95% CI = 0.94, 1.41) had higher hepatitis A rates. Counties with scores reflecting more married adults, residential stability, and lower income inequality despite disability, poverty, and low education (RR = 0.77; 95% CI = 0.59, 1.00) had lower hepatitis A rates. Counties with a higher percentage of workers in the manufacturing industry had slightly lower rates (RR = 0.97; 95% CI = 0.94, 1.00).

Conclusions. As expected, impoverished counties had higher hepatitis A rates. Evaluation across the socioeconomic patterns highlighted community-level factors (e.g., residential instability, income inequality, and social structures) that can be collected to augment hepatitis A data surveillance and used to identify higher-risk communities for targeted immunizations.
GEOPHYSICS OF ENTANGLED STATES OF THE COVID-19 PANDEMIC AND QUANTUM TELEPORTATION OF SARS COV-2 IN THE PLANET'S BIOGEOSPHERE
Authors: Gavrilov Vladimir , Antipova Tatyana , Vlasov Yan , Ardatov Sergey , Ardatova Anastasia
Published: July 2020

In their previous works , leading their history since 1988, the authors of this article have repeatedly conceptually shown and experimentally verified the results of research on the teleportation of information between macro objects. Early author's works were performed during the existence of the Russian Federation – as a country called the Union of Soviet Socialist Republics (USSR). Some of which were marked "Top Secret" - links further down the text. Since they were performed under the supervision of the relevant special services and further "Department of external relations of the Russian Academy of Sciences". The authors used numerous examples to demonstrate the possibility of teleportation of information in macro-systems, including ecosystem, biogeocenotic levels, and then tissue and organism levels. Successful experimental verifications occurred only in cases when all the principles and rules laid down in the theory of quantum information, applied to biological objects, were correctly combined. Namely, the preparation of cascades of entangled States was performed both on the mental and somatic levels. In full accordance with the principle of complementarity and taking into account the fact that the observer and the observed are actively connected by the sum of similarities. In addition, the role of the classical communication channel in this process was performed by carrier electromagnetic fields modulated by a useful signal. This signal represented a cast of the simulated experimental process. An example of a real COVID-19 pandemic is the verification of author's works in nature on a biogeocenotic scale. And certainly with anthropogenic – so to speak-participation.
ОРГАНИЗАЦИЯ ПРОТИВОЭПИДЕМИЧЕСКИХ МЕРОПРИЯТИЙ В ГВИНЕЙСКОЙ РЕСПУБЛИКЕ ПРИ БОРЬБЕ C COVID-19.
Authors: Крицкий Андрей
Published: July 2020

В статье представлен опыт по организации комплекса противоэпидемических мероприятий на территории Республики Гвинея в период с января по июнь 2020 года.
Extra-Articular Hip Resection and Reconstruction with Custom Acetabular Resection Guide and Implants in a Case of High-Grade Spindle Cell Sarcoma of the Proximal Femur
Authors: Manrique Jorge , Schiffman Corey J. , Chan Albert D. , Olsen David , Thompson Matthew J.
Published: July 2020

Case:

A 29-year-old man was admitted with acute atraumatic left hip pain and inability to bear weight. Subsequent workup revealed an intracapsular pathologic fracture of the femoral neck secondary to a high-grade spindle cell sarcoma. A unique method of extra-articular resection and reconstruction using a 3-dimensional (3D)-printed custom cutting jig and a custom acetabular component was pursued. Wide margins were achieved without violating the joint capsule but preserving pelvic integrity, allowing a return to an active lifestyle.

Conclusion:

Three-dimensional-printed custom resection guides and implants can help achieve adequate resection margins while preserving pelvic integrity and function.
Delayed Radial Nerve Palsy After Nonoperative Treatment of Humeral Shaft Fractures
Authors: Kelly Erin A. , Walden Timothy , Gross Jonathan , Egol Kenneth A.
Published: July 2020

Case:

Two patients who developed radial nerve palsy at least 6 weeks after injury during nonoperative treatment of humeral shaft fractures. This complication was associated with external bracing, progressive varus angulation during treatment, and excess callus formation.

Conclusion:

Delayed radial nerve palsy may develop during nonoperative treatment of humeral shaft fractures when functional bracing fails to maintain alignment and stability at the fracture site.
Ambulatory blood pressure is better associated with target organ damage than clinic blood pressure in patients with primary glomerular disease
Authors: wen ruowei , chen xiaoqiu , zhu ye , ke jianting , du yi , wang cheng
Published: July 2020

Background: Blood pressure is an important and modifiable cardiovascular risk factor. Ambulatory blood pressure monitoring (ABPM) provides valuable prognostic information in patients with chronic kidney disease (CKD), yet little is known about the association of various types of BP measurements with target organ damage (TOD) in patients with primary glomerular disease. The goal of this study was to investigate whether ambulatory blood pressure is better associated with TOD than clinic blood pressure in patients with primary glomerular disease. Methods: 1178 patients with primary glomerular disease were recruited in this cross-sectional study. TOD were assessed by the following 4 parameters: left ventricular mass index (LVMI or LVH, left ventricular hypertrophy), estimated glomerular filtration rate (eGFR<60ml/min/1.73m 2 ), albumin-to-creatinine ratio (ACR≥30mg/g) and carotid intima-media thickness (cIMT) or plaque. Receiver operating characteristic (ROC) curve and multivariate logistic regression analyses were used to evaluate the relationship between ambulatory or clinic systolic blood pressure (SBP) indexes and TOD. Results: Among 1178 patients (mean age, 39 years,54% men), 116, 458, 1031 and 251 patients had LVH, eGFR < 60 ml/min/1.73m 2 , ACR≥30mg/g and cIMT≥0.9mm or plaque respectively. Area under ROC curves for TOD in ambulatory SBP, especially nighttime SBP, was greater than that in clinic SBP ( P <0.05). Multivariate logistic regression analyses showed that 24h SBP, daytime SBP and nighttime SBP were significantly associated with LVH, eGFR<60 ml/min/1.73m 2 and ACR≥30mg/g after adjustment for clinic SBP, while the association of clinic SBP was attenuated after further adjustment for nighttime SBP. Conclusions: Ambulatory blood pressure, especially nighttime blood pressure, is probably superior to clinic blood pressure in predicting TOD in patients with primary glomerular disease.
The prevalence and immunological features of anti-glomerular basement membrane antibody in patients with HIV
Authors: Wang Wenjing , Jia Xiao-yu Jia , Cui Zhao , Chen Yan , Wang Wei , Lou Jin-li , Zhao Ming-hui , Ying Sun
Published: July 2020

Background: Anti-glomerular basement membrane disease (GBM) is a kind of chronic autoimmune disease caused by the deposition of circulating anti-GBM antibodies. Non-collagen region of α3 chain of type IV collagen (α3(IV)NC1) is one of the main target antigens. And on α3, EA and EB are the most classical antigen epitopes. It has been reported that anti-GBM antibodies can be detected in HIV patients, but its immunological characteristics are still unclear. In this study, the immunological characteristics of the target antigens were clarified. Methods: Total 93 HIV patients and 20 healthy volunteers were selected in Beijing Youan Hospital from 2017 to 2018. Recombinant human α1-α5(IV)NC1, chimeric protein EA and EB were used as solid phase antigens. Enzyme-linked immunosorbent assay was employed to measure concentrations and subtypes of serum IgG autoantibodies specifically against GBM.Results: Five out of the 93 patients with HIV had low to moderate levels of anti-GBM antibodies. However, these patients presented with no clinical manifestation of any kidney injury or pulmonary hemorrhages. Compared with HIV patients with negative antibodies, there were no significant differences in gender, age, CD4+T cell count and HIV viral load. All sera of five patients recognized non-collagenous domain1 (NC1) of alpha 3 chain of type IV collagen [(α3(IV)NC1] as classic anti-GBM patients, followed by α5(IV)NC1. The antibodies against α3(IV)NC1 were IgG3 predominant, but did not react with either of the classic epitopes on α3 (EA and EB).Conclusion: These data suggest a distinct immunological profile of anti-GBM antibodies in patients with HIV, and might explain the non-pathogenic features of HIV associated anti-GBM antibodies.
Occupational injuries, daily workload, and fitness levels among fitness and swimming instructors.
Authors: Bonato Matteo , Merati Giampiero , Agnello Luca , Grevers Dorothée , Maggioni Martina Anna
Published: July 2020

Background. Fitness (FI) and swimming instructor (SI) are two different type of trainers that are usually employed in fitness centers and their profession is highly physically demanding because they have to practice while teaching. To date, data on occupational disorders in FI are limited and there is no information about SI. Therefore, the primary outcome of this study was an exploratory analysis of occupational disorders experienced by FI and SI employed in various fitness centers through a self-reported questionnaire, to provide first data on its prevalence. The secondary outcome was to assess fitness levels, workloads and perceived exertion during a typical working day of SI and FI in order to explore the possible factors associated with occupational disorders in these professional activities. Methods. An online cross-sectional survey addressing physical data, frequency, duration and time of class participation and self-reported occupational disorders was conducted among 435 subjects (FI=254; SI=179). Subsequently, 97 subjects (FI=57; SI=42), were enrolled to investigate physical fitness by maximal oxygen consumption ( O2max), and daily workload by heart rate (HR) and rating of perceived exertion (RPE). Results. A total of 621 musculoskeletal disorders and 521 of other disorders were included in the study with 157 and 155 participants that experienced 2 or more injuries during their career respectively. FI exhibited a higher prevalence of musculoskeletal injuries and SI experienced more upper respiratory tract infections. O2max ranged from 47.0±4.0 to 51.9±3.7 ml·kg-1·min-1, and no statistically significant differences between sex and instructor type was found. No statistical differences between instructor type in HRmean and HRmean/HRmax was found. According to sex, female SI displayed significantly higher HRmean and HRmean/HRmax (p=0.018, p=0.022, respectively) than female FI. Conclusions. In conclusion, FI and SI experienced a high prevalence of musculoskeletal and occupational disorders during their professional career. Moreover, data on FI and SI physical fitness and daily workload monitoring will help to set up guidelines regarding maximum weekly teaching load.
Differential diagnostic value of total alpha-synuclein assay in the cerebrospinal fluid between Alzheimer's disease and dementia with Lewy bodies from the prodromal stage
Authors: BOUSIGES Olivier , Philippi Nathalie , Lavaux Thomas , Perret-Liaudet Armand , Lachmann Ingolf , Schaeffer-Agalède Caroline , Anthony Pierre , Botzung Anne , Rauch Lucie , Jung Barbara , de Sousa Paulo Loureiro , Demuynck Catherine , Martin-Hunyadi Catherine , Cretin Benjamin , Blanc Frédéric
Published: July 2020

Background: Several studies have investigated the value of alpha-synuclein assay in the cerebrospinal fluid (CSF) of Alzheimer’s disease (AD) and dementia with Lewy bodies (DLB) patients in the differential diagnosis of these two pathologies. However, very few studies have focused on this assay in AD and DLB patients at the MCI stage.Methods: All patients were enrolled under a hospital clinical research protocol from the tertiary Memory Clinic (CM2R) of Alsace, France, by an experienced team of clinicians. A total of 166 patients were included in this study: 21 control subjects (CS), 51 patients with DLB at the prodromal stage (pro-DLB), 16 patients with DLB at the demented stage (DLB-d), 33 AD patients at the prodromal stage (pro-AD), 32 AD patients at the demented stage (AD-d) and 13 patients with mixed pathology (AD+DLB). CSF levels of total alpha-synuclein were assessed using a commercial enzyme-linked immunosorbent assay (ELISA) for alpha-synuclein (AJ Roboscreen). Alzheimer’s biomarkers (t-Tau, P-Tau, Aβ42 and Aβ40) were also measured.Results: The alpha-synuclein assays showed a significant difference between the AD and DLB groups. Total alpha-synuclein levels were significantly higher in AD patients than in DLB patients. However, the ROC curves show a moderate discriminating power between MA and DLB (AUC = 0.78) which does not improve the discriminating power of the combination of Alzheimer biomarkers (AUC = 0.95 with or without alpha-synuclein). Interestingly, the levels appeared to be altered from the prodromal stage in both AD and DLB.Conclusions: The modification of total alpha-synuclein levels in the CSF of patients occurs early, from the prodromal stage. The adding of alpha-synuclein total to the combination of Alzheimer’s biomarker does not improve the differential diagnosis between AD and DLB.Trial registration: ClinicalTrials.gov, (AlphaLewyMa, Identifier: NCT01876459)
Factors affecting bone maturation in Chinese girls aged 4-8 years with isolated premature thelarche
Authors: Su Huiping , Su Zhe , Pan Lili , Wang Li , Xu Zhongwei , Peng Gang , Li Xianglei
Published: July 2020

Background: In isolated premature thelarche (IPT) girls, bone age (BA) is considered consistent with chronological age. However, some IPT girls confirmed by gonadotropin-releasing hormone (GnRH) stimulation test could show another trend. We analysed BA and possible potentiating factors in a selected group of girls aged 4-8 years with IPT. Methods: IPT girls confirmed by GnRH stimulation test aged 4-8 years hospitalized from January 2015 to April 2018 at Shenzhen Children's Hospital were included in this retrospective study. They were divided into two groups with advanced BA of 2 years as the cut-off. Body mass index (BMI) and hormone levels were the main outcome measures, and regression analysis was used to identify independent risk factors. IPT girls were divided into subgroups according to the levels of BMI standard deviation score (SDS), insulin-like growth factor-1 (IGF-1) SDS and dehydroepiandrosterone sulfate (DHEAS) SDS for comparisons of advanced BA. Results: Overall, 423 subjects were included and classified into the advanced BA group (48.7%, n=206) and control group (51.3%, n=217). The advanced BA group had significantly higher BMI SDS, serum DHEAS SDS, IGF-1 SDS, androstenedione and fasting insulin and significantly lower sex hormone binding globulin (all p<0.001). Serum IGF-1 SDS (OR=1.926, p＜0.001), BMI SDS (OR=1.427, p=0.001) and DHEAS SDS (OR=1.131, p=0.005) were independent risk factors for significantly advanced BA. In the multiple linear regression model, serum IGF-1 SDS, BMI SDS and DHEAS SDS were the strongest predictors of advanced BA, accounting for 19.3% of the variance. According to BMI, 423 patients were classified into three groups: normal weight (56.03%, n=237), overweight (19.15%, n=81) and obesity (24.82%, n=105). The proportion of advanced BA in obesity group was significantly higher than those of normal weight and overweight groups (χ2=18.088, p＜0.001). In the subgroup with normal weight, higher serum IGF-1 SDS (p=0.009) and DHEAS SDS (p=0.003) affected BA advancement independent of BMI SDS. Conclusions: Girls with IPT confirmed by GnRH stimulation test aged 4-8 years might have significantly advanced BA. Obesity was highly associated with advanced BA. Age-specific serum IGF-1 SDS and DHEAS SDS were risk factors for BA advancement independent of BMI.
ABT-263 enhanced bacterial phagocytosis of macrophages in aged mouse through Beclin-1-dependent autophagy
Authors: Zhang Yu , Tang Li-hua , Lu Jia , Xu Li-ming , Cheng Bao-li , Xiong Junyu
Published: July 2020

Background: Sepsis is a critical challenge for the elderly population as the immune function is less responsive by aging. Although cell numbers seem preserved in the elderly, macrophages present age-related function decline, which including reduced chemokines, phagocytosis, and autophagy. ABT-263, an inhibitor of the anti-apoptotic protein Bcl-2, is reported had a senolytic effect which can selectively clear the senescent cells in vivo and rejuvenate the aged tissues.Methods: We treated the aged (12-16 months) and young (4-6 months) C57BL/6 mouse with ABT-263, then gave the animals cecal slurry injection to induce sepsis to observe the effect of senolytic compound ABT-263 on the survival rate of sepsis. Additionally, we isolated peritoneal macrophages from the aged mouse to investigate the cell function and molecular mechanism. 3-methyladenine (3-MA), a phosphatidylinositol 3-kinases (PI3K) inhibitor, and rapamycin, an autophagy-enhancer, were used to block or mimic the autophagy, respectively. RT-PCR and Western Blot were used to detect autophagy related gene and protein changes in sepsis. EGFP-expressing E. Coli was used as a marker to evaluate the phagocytic ability of macrophages. Results: The results showed ABT-263 treatment improved the survival rate of sepsis in the aged mouse which related to autophagy, while blocking the autophagy can eliminate this effect. It is revealed that ABT-263 enhanced the phagocytic ability of the peritoneal macrophages by increasing the Trem-2 receptor. Additionally, ABT-263 blocked the binding of Bcl-2 to Beclin-1, thus induced Beclin-1-dependent autophagy. Conclusion: ABT-263 enhanced the macrophage function in aged mouse by increasing the Trem-2 receptors and inducing a beclin-1-dependent autophagy, consequently, protected the aged mouse from sepsis.
Scope of a weekly infection control team rounding in an acute-care teaching hospital: a pilot study
Authors: Jeong Yeon Su , Kim Jin Hwa , Lee Seungju , Lee So Young , Oh Sun Mi , Lee Eun Jung , Kim Tae Hyong , Park Se Yoon
Published: July 2020

Regular and well-organized inspection of infection control is an essential element of an infection control program. The aim of this study was to identify the functional scope of weekly infection control team rounding (ICTR) in an acute care hospital. We conducted weekly ICTR between January 18 and December 26, 2018 to improve the compliance to infection control and prevention measures at a 734-bed academic hospital in the Republic of Korea and analyzed the results retrospectively. We categorized the results into five groups: “well maintained,” “improvement needed,” “long-term support, such as space or manpower, needed,” “not applicable,” or “could not be observed”. A total of nine categories and 85 sub-elements of infection control and prevention practices were evaluated. The median number of infection control team (ICT) visits per department was 7 (interquartile range [IQR]: 6-7). The ICT assessed a median of 16 elements (IQR: 12-22), and a total of 7,452 results were obtained. Of those, 75% were monitored properly, 22% were “not applicable”, and 4% were difficult to observe. The most common practices that were difficult to observe were strategies to prevent catheter-related surgical site infections, pneumonia, and occupationally acquired infections as well as injection safety practices. Although the ICTR was able to maintain regular visits to each department, further strategies beyond regular ICTR are needed to reduce category of “could not observed”. This pilot study may provide an important reference for institutional infection prevention practices as it is the first study to investigate the functional coverage of ICTR.
Acute hospital staff’s attitudes towards dementia and perceived dementia knowledge: a cross-sectional survey in Ireland
Authors: Keogh Brian , To Wing Ting , Daly Louise , Hynes Geralyn , Kennelly Siobhan , Lawlor Brian , Timmons Suzanne , O’Reilly Susan , Bracken-Scally Mairead , Ciblis Aurelia , Cole Natalie , Drury Amanda , Pittalis Chiara , Kennelly Brendan , McCarron Mary , Brady Anne-Marie
Published: July 2020

Background: Little is known about staff’s attitudes in Irish acute hospital settings towards people living with dementia and their perceived dementia knowledge. The aim objective of this study was to understand the general level of dementia knowledge and attitudes towards dementia in different types of hospital staff, as well as to explore the potential influence of previous dementia training and experience (having a family member with dementia) and the potential moderating effects of personal characteristics. This data was required to plan and deliver general and targeted educational interventions to raise awareness of dementia throughout the acute services. Methods: A cross-sectional survey was carried out among a diverse range of hospital staff (n = 1795) in three urban acute general hospitals in Ireland, including doctors, nurses, healthcare attendants, allied professionals, and general support staff. Participants’ perceived dementia knowledge and attitudes were assessed as well as their previous dementia training and experience. To measure participant’s attitude towards dementia, the validated Approaches to Dementia Questionnaire (ADQ) was used.Results: Hospital staff demonstrated positive attitudes towards people living with dementia, and believed they had a fair to moderate understanding of dementia. Both ‘having previous dementia training’ and ‘having a relative living with dementia’ predicted attitude towards dementia and perceived dementia knowledge. Interestingly, certain personal staff characteristics did impact dementia training in predicting attitude towards dementia and perceived dementia knowledge.Conclusion: This study provides a baseline of data regarding the attitudes towards dementia and perceived dementia knowledge for hospital staff in Irish acute hospitals. The results can inform educational initiatives that target different hospital staff, in order to increase awareness and knowledge to improve quality of dementia care in Irish hospitals.
Antimicrobial resistance and toxigenic profiles of bacteria isolated from tropical shrimps (Farfantepenaeus notialis and Penaeus monodon) in Cameroun
Authors: OMBEDE Sabine Ninelle NGA , DOUGNON Victorien Tamègnon , KOUDOKPON Hornel , DEGUENON Esther , NGOMO Rajeunie Pernelle Jaelle MINDZIE , TCHIBOZO Carine , GNIMATIN Jean Pierre , TCHOUMBOUGNANG François , YADOULETON Anges , DOUGNON Jacques
Published: July 2020

Objective: Post-harvest shrimp losses are a big problem due to the proliferation of spoilage bacteria. Presence and multiplication of these bacteria promotes the emergence of food-borne illnesses. This study was carried out to characterize specifics spoilage bacteria from tropical brackish water shrimp and black tiger shrimp shrimps. Result: 22 Bacillus spp ; 09 SCNs staphylococcus and 04 enterobacteria such as 01 Pantoea genus ; 01 Serratia plymutica and 02 Serratia rubidaea have been identified. Resistance and virulence genes were searched. All isolates expressed resistance to at least three of antibiotics tested. 03 strains of enterobacteria were sensible to cetfazidim and Amoxicillin-Clavulanic Acid . Bacillus showed complete susceptibility to Cefixim, ertapenem and cetfazidim. Staphylocococci were susceptible to clyndamycine. Pantoea spp was resistant to all antibiotics but exhibited intermediate susceptibility to Amoxycyclin-Clavulanic Acid. 04 strains of Staphycolocci were positive to mecA genes. None enterobacteria were positives to tetracycline resistance genes. All the strains of Bacillus contained enterotoxin genes, high prevalence of 21 strain to hemolytic enterotoxins and 17 strains to cell-lyse factor production like sphingomyelinase activities. The majority of Bacillus strains present in the study poses a potential risk of food poisoning due to the prevalence of toxin genes found.
Impact of prematurity and the CTG repeat length on outcomes in congenital myotonic dystrophy
Authors: Saito Yu , Matsumura Kenta , Kageyama Misao , Kato Yuichi , Ohta Eiji , Sumi Kiyoaki , Futatani Takeshi , Yoshida Taketoshi
Published: July 2020

Objective: Patients with congenital myotonic dystrophy (CDM) tend to be born preterm. Although the CDM severity generally depends on the CTG repeat length, prematurity may also affect the prognosis in patients with CDM. Given that preterm birth is expected to increase the risk of CDM in newborns, we investigated the outcomes of newborns with CDM according to gestational age to assess prematurity and the CTG repeat length for predicting prognosis. Results: We assessed the outcomes of 54 infants with CDM using data collected from our hospitals and previously published studies. The patients were divided into mild and severe groups based on clinical outcomes. Logistic regression analysis was performed to estimate odds ratios (ORs) for CDM prognosis according to gestational age and the CTG repeat length and to construct a predictive model. Logistic regression analysis showed both the CTG repeat and gestational age were significantly associated with severe outcomes in patients with CDM (OR: 32.27, 95% CI: 3.45–300.7; p = .002 and OR: 0.73, 95% CI: 0.58–0.93; p = .0094, respectively). This predictive model for CDM prognosis exhibited good sensitivity (63%) and specificity (86%). Both prematurity and the CTG repeat length were significantly associated with the CDM severity.
Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury
Authors: Sierra-Parraga Jesus Maria , Merino Ana , Eijken Marco , Leuvenink Henri , Ploeg Rutger J. , Møller Bjarne K. , Jespersen Bente , Baan Carla C. , Hoogduijn Martin J.
Published: July 2020

Background: The renal endothelium is a prime target for ischemia reperfusion injury (IRI) during donation and transplantation procedures. Mesenchymal stromal cells (MSC) have been shown to ameliorate kidney function after IRI. However, whether this involves repair of the endothelium is not clear. Therefore, our objective is to study potential regenerative effects of MSC on injured endothelial cells and to identify the molecular mechanisms involved.Methods: Human umbilical vein endothelial cells (HUVEC) were submitted to hypoxia and reoxygenation and TNF-a treatment. To determine whether physical interaction or soluble factors released by MSC were responsible for the potential regenerative effects of MSC on endothelial cells, dose-response experiments were performed in co-culture and transwell conditions and with secretome deficient MSC. Results: MSC showed increased migration and adhesion to injured HUVEC, mediated by CD29 and CD44 on the MSC membrane. MSC decreased membrane injury marker expression, oxidative stress levels and monolayer permeability of injured HUVEC, which was observed only when allowing both physical and paracrine interaction between MSC and HUVEC. Furthermore, viable MSC in direct contact with injured HUVEC improved wound healing capacity by 45% and completely restored their angiogenic capacity. In addition, MSC exhibited an increased ability to migrate through an injured HUVEC monolayer compared to non-injured HUVEC in vitro. Conclusions: These results show that MSC have regenerative effects on injured HUVEC via a mechanism which requires both physical and paracrine interaction. The identification of specific effector molecules involved in MSC-HUVEC interaction will allow targeted modification of MSC to apply and enhance the therapeutic effects of MSC in IRI.
Validation of multiplex PCR sequencing assay of SIV
Authors: Moriarty Ryan V , Fesser Nico , Sutton Matthew S , Venturi Vanessa , Davenport Miles P , Schlub Timothy , O'Connor Shelby L
Published: July 2020

Background: The generation of accurate and reproducible viral sequence data is necessary to understand the diversity present in populations of RNA viruses isolated from clinical samples. While various sequencing methods are available, they often require high quality templates and high viral titer to ensure reliable data. Methods: We modified a multiplex PCR and sequencing approach to characterize populations of simian immunodeficiency virus (SIV) isolated from nonhuman primates. We chose this approach with the aim of reducing the number of required input templates while maintaining fidelity and sensitivity. We conducted replicate sequencing experiments using different numbers of quantified viral RNA (vRNA) or viral cDNA as input material. We performed assays with clonal SIVmac239 to detect false positives, and we mixed SIVmac239 and a variant with 24 point mutations (SIVmac239-24X) to measure variant detection sensitivity. Results: We found that utilizing a starting material of quantified viral cDNA templates had a lower rate of false positives and increased reproducibility when compared to that of quantified vRNA templates. This study identifies the importance of rigorously validating deep sequencing methods and including replicate samples when using a new method to characterize low frequency variants in a population with a small number of templates. Conclusions: Because the need to generate reproducible and accurate sequencing data from diverse viruses from low titer samples, we modified a multiplex PCR and sequencing approach to characterize SIV from populations from non-human primates. We found that increasing starting template numbers increased the reproducibility and decreased the number of false positives identified, and this was further seen when cDNA was used as a starting material. Ultimately, we highlight the importance of vigorously validating methods to prevent overinterpretation of low frequency variants in a sample.
The Influential factors of Adherence to Physical Activity and Exercise among Community-Dwelling Stroke Survivors: A Path Analysis
Authors: Lin Beilei , Zhang Zhenxiang , Mei Yongxia , Wang Wenna , Liu Lamei , Xu Hui
Published: July 2020

The authors have withdrawn this preprint due to erroneous posting.
Minimum Volume Standards in day Surgery: A Systematic Review
Authors: Stanak Michal , Strohmaier Christoph
Published: July 2020

Background: To find out if and for what indications are minimum volume standards (MVS) applied in the day surgery setting and whether the application of MVS improves patient relevant outcomes. Methods: We conducted a comprehensive systematic literature search in seven databases on July 12th, 2019. Concerning effectiveness and safety, the data retrieved from the selected studies were systematically extracted into data-extraction tables. Two independent researchers (MS, CS) systematically assessed the quality of evidence using the quality assessment tool for individual studies of the International Society for Pharmacoeconomics and Outcomes Research (ISPOR) called Task Force Checklist for Quality Assessment of Retrospective Database Studies. No instances of disagreement occurred. No further data processing was applied.Results: The systematic literature search, together with hand search, yielded 595 hits. No prospective or controlled studies were found. Data from eight retrospective studies were used in the analysis of clinical effectiveness and safety on seven indications: anterior cruciate ligament reconstruction, cataract surgery, me­niscectomy, thyroidectomy, primary hip arthroscopy, open carpal tunnel release, and rotator cuff repair. All interventions (except for carpal tunnel release and thyroidectomy) confirmed a volume-outcome relationship (VOR) with relation to surgeon/hospital volume, however, none established MVS for the respective interventions. Safety related data were reported without its relationship to surgeon/hospital volume.Conclusions: This present paper provides some evidence in favor of the VOR, however, it based on low quality retrospective data-analyses. The present results cannot offer any clear-cut MVS thresholds for the day surgery setting and so the simple transition from inpatient results (that support MVS) to the day surgery setting is questionable. Further quality assuring policy approaches should be considered.
Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells by suppressing the PI3K/Akt/mTOR signalling pathway
Authors: Kwakye Akosua Kotaa , Kampo Sylvanus , Lv Jiaxin , Ramzan Muhammad Noman , Richard Seidu A. , Falagán Aglais Arredondo , Agudogo Jerry , Atito-Narh Evans , Yan Qiu , Wen Qing-Ping
Published: July 2020

Objective This study aimed to test the hypothesis that levobupivacaine has anti-tumour effects on breast cancer cells Results Colony formation and transwell assay were used to determine breast cancer cells proliferation. Flow Cytometry (annexin V and PI staining) was used to investigate breast cancer cells apoptosis. The effects of levobupivacaine on cellular signalling and molecular response were studied with Quantitative Polymerase Chain Reaction and western blot. Induction of apoptosis was confirmed by cell viability, morphological changes showed cell shrinkage, rounding, and detachments from plates. The results of the western blot and Quantitative Polymerase Chain Reaction indicated activation of active caspase-3 and inhibition of FOXO1. The results of the flow Cytometry confirmed that levobupivacaine inhibited breast cancer cell proliferation and enhanced apoptosis of breast cancer cells. Quantitative Polymerase Chain Reaction and Western blot analysis showed increased p21 and decreased cyclin D. Quantitative Polymerase Chain Reaction and western blot analysis showed that levobupivacaine significantly increased Bax expression, accompanied by a significant decreased Bcl-2 expression and inhibition of PI3K/Akt/mTOR signalling pathway. These findings suggested that levobupivacaine inhibits proliferation and promotes breast cancer cells apoptosis in vitro.
Voltage controlled Néel vector rotation in zero magnetic field at CMOS-compatible temperatures
Authors: Mahmood Ather , Echtenkamp Will , Street Mike , Wang Jun-Lei , Cao Shi , Komesu Takashi , Dowben Peter , Buragohain Pratyush , Lu Haidong , Gruverman Alexei , Parthasaraty Arun , Rakheja Shaloo , Binek Christian
Published: July 2020

Multi-functional thin films of boron (B) doped Cr2O3 exhibit voltage-controlled and nonvolatile Néel vector reorientation in the absence of an applied magnetic field, H. Toggling of antiferromagnetic states is demonstrated in prototype device structures at CMOS compatible temperatures between 300 and 400 K. Boundary magnetization associated with the Néel vector orientation serves as state variable which is read via magnetoresistive detection in a Pt Hall bar adjacent to the B: Cr2O3 film. Switching of the Hall voltage between zero and negative values implies Néel vector rotation by 90-degree. Magnetometry, spin resolved inverse photoemission, electric transport measurements and piezo force microscopy reveal B-dependent TN and resistivity enhancement, spin-canting, anisotropy reduction and dynamic polarization hysteresis. Their combined effect enables H=0, voltage controlled, nonvolatile Néel vector rotation at high-temperature. Theoretical modeling estimates switching speeds of about 100ps making B: Cr2O3 a promising multifunctional single-phase material for energy efficient nonvolatile CMOS compatible memory applications.