bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Pemanfaatan APBD Kota Yogyakarta dalam Pengembangan Aplikasi Jogja Smart Service: Studi Kualitatif Deskriptif
Authors: Anggrayni Irena , Devandra Marsheila Hera
Published: August 2024

Dalam penelitian ini akan lebih mengulik bagaimana pengalokasian dana APBD serta bentuk ilmpementasi yang dilakukan DISKOMINFOSAN dalam pemanfaatan aplikasi Jogja Smart Service. Penelitian ini menggunakan metode deskriptif kualitatif dengan teknik pengumpulan data melalui observasi, wawancara dan dokumentasi dengan menggunakan teori yang dikemukakan oleh Indrajit  menyebutkan bahwa ada 3 elemen sukses dalam pengembangan E-Government berdasarkan kajian yang dilakukan oleh Harvard JFK School Of Government dengan memfokuskan pada elemen dukungan, elemen kapasitas dan elemen nilai (Indrajit, 2001) dengan 3 elemen antara lain: elemen pendukung, elemen kapasistas dan elemen nilai. Hasil penelitian menunjukan bahwasannya dalam pengalokasian dana APBD Kota Yogyakarta mulai dari tahun anggaran 2019 sebesar Rp. 2.260.970.585 sampai tahun 2023 sebesar Rp. 43.079.079.458 sudah terimplementasi dengan baik berdasar pada elemen pendukung yaitu pemerintah dengan pengesahan peraturan tentang digitalisasi pemerintah, kemudan elemen kapasitas berkaitan dengan finansial (dukungan APBD), fasilitas (jaringan wifi dan perangkat lunak) dan sumber daya manusia. Sehingga aplikasi JSS ini dapat digunakan oleh masyarakat secara mudah dan cepat, aplikasi JSS ini pula dalam implemntasinya mendapat penghargaan antara lain: top inovasi pelayanan pelayanan publik nasional dmana JSS ini masuk dalam top 45 inovasi pelayanan publik, JSS ini mewakili Indonesia dalam kompetensi layanan publik internasional masuk pada jajaran 11 nominasi United Nation of Public Service Award (UNPSA). Namun begitu masih ada saya kekurangan yang terjadi sepertin kendala jaringan, sosialisasi serta perangkat yang kurang mumpuni.
Kata Kunci : Anggaran, Jogja Smart Service, Implementasi
Hubungan Pengetahuan, Sikap, dan Ketersediaan Sarana dan Prasarana terhadap Perilaku Pemilahan Sampah pada Pedagang di Pasar Agung Kota Depok
Authors: Rozni Zahira Hananda Naila , Sulistyorini Desy
Published: August 2024

The waste problem in Indonesia is exacerbated by people's awareness and their actions regarding healthy lifestyles and a clean, waste-free environment. Many people do not realize the importance of waste processing, but there are also many people who understand the importance of waste processing but do not carry out this behavior. The researcher's aim is to determine the relationship between knowledge, attitudes, and availability of facilities and infrastructure on waste sorting behavior among traders at Pasar Agung, Depok City in 2023. Quantitative research method with a cross-sectional research design. The population in this research is all traders at Pasar Agung Depok City. The sampling technique was carried out using probability sampling and 78 samples were obtained. The data used is primary data distributed through a questionnaire. The research results show a p value of 0.027 (p > 0.05), meaning that there is a relationship between knowledge and waste sorting behavior, there is a relationship between attitude and waste sorting behavior, p value 0.020 (p > 0.05), conclusion : and there is a relationship between the availability of facilities and infrastructure and behavior. waste sorting p value 0.046 (p > 0.05). Suggestions from researchers hope that traders can separate waste according to type (organic and non-organic). And it is also hoped that traders can choose packaging that is used for the long term rather than single-use packaging. Traders can also choose more environmentally friendly materials, think and reconsider before purchasing an item.
Sex-specific alterations in the gut and lung microbiome of allergen-induced mice
Authors: Ekpruke Carolyn Damilola , Alford Rachel , Rousselle Dustin , Babayev Maksat , Sharma Shikha , Parker Erik , Davis Kyle , Hemmerich Christopher , Rusch Douglas B. , Silveyra Patricia
Published: August 2024

Introduction

Recent evidence has demonstrated that the microbiome is a driver of the underlying pathophysiological mechanisms of respiratory disease. Studies have indicated that bacterial metabolites produced in the gut and lung can impact lung inflammation and immune cell activity, affecting disease pathology. Despite asthma being a disease with marked sex differences, experimental work linking microbiomes and asthma has not considered the sex variable.

Methods

To test the hypothesis that the lung and gut microbial composition impacts allergic lung inflammation in a sex-specific manner, we evaluated lung and gut microbiome alterations in a mouse model of allergic inflammation and assessed their association with lung function and inflammation phenotypes. For this, we exposed male and female adult C57BL/6J mice intranasally to 25 µg of a house dust mite extract mix (HDM) daily, or phosphate-buffered saline (PBS) as control, for 5 weeks (n = 4–6/group). DNA from fecal pellets collected before and after the 5-week treatment, and from lung tissue collected at endpoint, was extracted using the ZymoBIOMICS®-96 MagBead DNA Kit and analyzed to determine the 16S microbiome via Targeted Metagenomic Sequencing.

Results

The HDM treatment induced a sex-specific allergic inflammation phenotype with significantly higher neutrophilia, lymphocytosis, inflammatory gene expression, and histopathological changes in females than males following exposure to HDM, but higher airway hyperresponsiveness (AHR) in males than females. In addition, sex-specific lung gene expression and associated pathways were identified HDM mix after challenge. These changes corresponded to sex-specific alterations in the gut microbiome, where the Firmicutes to Bacteroidetes ratio (F:B) was significantly reduced in fecal samples from only male mice after HDM challenge, and alpha diversity was increased in males, but decreased in females, after 5-weeks of HDM treatment.

Discussion

Overall, our findings indicate that intranasal allergen challenge triggers sex-specific changes in both gut and lung microbiomes, and induces sex-specific lung inflammation, AHR, and lung inflammatory gene expression pathways, suggesting a contribution of the lung-gut axis in allergic airway disease.
Cardiac tissue engineering: an emerging approach to the treatment of heart failure
Authors: Rayat Pisheh Hossein , Nojabaei Fatemeh Sadat , Darvishi Ahmad , Rayat Pisheh Ali , Sani Mahsa
Published: August 2024

Heart failure is a major health problem in which the heart is unable to pump enough blood to meet the body’s needs. It is a progressive disease that becomes more severe over time and can be caused by a variety of factors, including heart attack, cardiomyopathy and heart valve disease. There are various methods to cure this disease, which has many complications and risks. The advancement of knowledge and technology has proposed new methods for many diseases. One of the promising new treatments for heart failure is tissue engineering. Tissue engineering is a field of research that aims to create living tissues and organs to replace damaged or diseased tissue. The goal of tissue engineering in heart failure is to improve cardiac function and reduce the need for heart transplantation. This can be done using the three important principles of cells, biomaterials and signals to improve function or replace heart tissue. The techniques for using cells and biomaterials such as electrospinning, hydrogel synthesis, decellularization, etc. are diverse. Treating heart failure through tissue engineering is still under development and research, but it is hoped that there will be no transplants or invasive surgeries in the near future. In this study, based on the most important research in recent years, we will examine the power of tissue engineering in the treatment of heart failure.
Molecular characteristics and pathogenic mechanisms of KPC-3 producing hypervirulent carbapenem-resistant Klebsiella pneumoniae (ST23-K1)
Authors: Tu Yanye , Gao Hui , Zhao Rongqing , Yan Jiliang , Wu Xingbing
Published: August 2024

Objective

This study aimed to comprehensively investigate hypervirulent carbapenem-resistant Klebsiella pneumoniae (CR-hvKP) in the Ningbo region. Importantly, we sought to elucidate its molecular characteristics and pathogenic mechanisms. This information will provide evidence-based insights for preventing and controlling nosocomial infections and facilitate improved clinical diagnosis and treatment in this region.

Methods

96 carbapenem-resistant Klebsiella pneumoniae strains were collected from the Ningbo region between January 2021 and December 2022. Whole genome sequencing and bioinformatic methods were employed to identify and characterize CR-hvKP strains at the molecular level. The minimum inhibitory concentrations (MICs) of common clinical antibiotics were determined using the VITEK-2 Compact automatic microbiological analyzer. Plasmid conjugation experiments evaluated the transferability of resistance plasmids. Finally, mouse virulence assays were conducted to explore the pathogenic mechanisms.

Results

Among the 96 strains, a single CR-hvKP strain, designated CR-hvKP57, was identified, with an isolation frequency of 1.04%. Whole-genome sequencing revealed the strain to be ST23 serotype with a K1 capsule. This strain harbored three plasmids. Plasmid 1, a pLVPK-like virulence plasmid, carried multiple virulence genes, including rmpA, rmpA2, iroB, iucA, and terB. Plasmid 2 contained transposable element sequences such as IS15 and IS26. Plasmid 3, classified as a resistance plasmid, harbored the blaKPC-3 carbapenem resistance gene. Mouse virulence assays demonstrated a high mortality rate associated with CR-hvKP57 infection. Additionally, there was a significant increase in IL-1β, IL-6, and TNF-α levels in response to CR-hvKP57 infection, indicating varying degrees of inflammatory response. Western blot experiments further suggested that the pathogenic mechanism involves activation of the NF-κB signaling pathway.

Conclusion

This study confirms the emergence of hypervirulent CR-hvKP in the Ningbo region, which likely resulted from the acquisition of a pLVPK-like virulence plasmid and a blaKPC-3 resistance plasmid by the ST23-K1 type Klebsiella pneumoniae. Our findings highlight the urgent need for more judicious use of antibiotics to limit the emergence of resistance. Additionally, strengthening infection prevention and control measures is crucial to minimize the spread of virulence and resistance plasmids.
Research progress on the pattern recognition receptors involved in porcine reproductive and respiratory syndrome virus infection
Authors: Xu Yulin , Ding Luogang , Zhang Yuyu , Ren Sufang , Li Jianda , Liu Fei , Sun Wenbo , Chen Zhi , Yu Jiang , Wu Jiaqiang
Published: August 2024

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating infectious diseases of pigs globally. The pathogen, porcine reproductive and respiratory syndrome virus (PRRSV), is an enveloped positive-stranded RNA virus, which is considered to be the key triggers for the activation of effective innate immunity through pattern recognition receptor (PRR)-dependent signaling pathways. Toll-like receptors (TLRs), RIG-I-like receptors (RLRs), C-type lectin receptors (CLRs), NOD-like receptors (NLRs) and Cytoplasmic DNA receptors (CDRs) are used as PRRs to identify distinct but overlapping microbial components. The innate immune system has evolved to recognize RNA or DNA molecules from microbes through pattern recognition receptors (PRRs) and to induce defense response against infections, including the production of type I interferon (IFN-I) and inflammatory cytokines. However, PRRSV is capable of continuous evolution through gene mutation and recombination to evade host immune defenses and exploit host cell mechanisms to synthesize and transport its components, thereby facilitating successful infection and replication. This review presents the research progress made in recent years in the study of these PRRs and their associated adapters during PRRSV infection.
An in-depth study on survival mechanism of bacterial isolates in disinfectants within the hospital environment
Authors: Rakshit Pue , Singh Aradhana , Singh Ravindra , Banerjee Tuhina
Published: August 2024

Introduction

The emergence of disinfectant resistance has become a severe threat due to reduced effectiveness. This study was undertaken to determine how bacteria adapt to survive exposure to disinfectants in the busiest section of a tertiary care hospital in Varanasi, India.

Methods

Four isolates (two Klebsiella pneumoniae, Kp1 and Kp2; two Pseudomonas aeruginosa, Pa1 and Pa2) were obtained from chlorhexidine (CHX)–based handwash during microbiological surveillance of “in-use disinfectants” in hospital. Six disinfectants [4% CHX, 2% glutaraldehyde, 7.5% hydrogen peroxide, 1% sodium hypochlorite and 0.1% benzalkonium chloride (BAC), and 70% ethyl alcohol] were tested against these four isolates to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Antibiotic profile, change in MIC on exposure to disinfectants and biofilm formation in the presence and absence of disinfectants was studied. Whole genome sequencing (WGS) was done to identify the resistance mechanisms.

Result

The isolates showed the highest MBC/MIC ratio (4) against glutaraldehyde. Exposure to supra-inhibitory concentration of BAC for 21 days resulted in doubling of MIC/MBC. The majority (75%) of the isolates were multidrug resistant. All the isolates were strong biofilm producers. The reduction rate of biofilm formation decreased with an increase in the concentration of disinfectants (p = 0.05 for BAC). WGS revealed multiple AMR genes including blaDIM-1, disinfectant-resistant gene and efflux pump genes.

Conclusion

The study emphasized the various adaptation strategies of these isolates for survival in disinfectant environment, thus posing a huge challenge for their control in the hospital environment.
Cognitive function, mood and sleep changes in response to a Tai Chi/Qigong intervention among older breast cancer survivors: an exploratory analysis
Authors: James Dara L. , Maxfield Molly , Han SeungYong , Hawley Nanako A. , Petrov Megan E. , Sears Dorothy D. , Vance David E. , Larkey Linda K.
Published: August 2024

Introduction

Cognitive decline is a significant, persistent issue among breast cancer survivors (BCSs) affecting more than 50% and greatly impacting health and wellbeing, particularly among those who are aging. Scalable, lifestyle interventions to mitigate cognitive decline in this population are needed. This study aimed to explore the effects of Tai Chi Easy (TCE) on perceived cognition function among older BCSs.

Methods

The current work was part of a larger NCI-funded trial (R01CA182901, primary results reported elsewhere). Female BCSs, aged 45–75 years, were randomized to one of three conditions: two active interventions, Tai Chi Easy (TCE) or sham Qigong (SQG), or an education control group (EDC). In this exploratory analysis of older female participants (aged 60–75y), perceived cognitive function and performance and related factors (including anxiety, depression, and insomnia symptom severity) were examined. We anticipated TCE and SQG would show greater improvements in perceived cognitive function and performance compared to EDC.

Results

A total of 75 female BCS were included in the analysis (TCE = 23; SQG = 22; EDC = 30). Linear mixed model results showed BCS randomized to TCE and SQG reported improvements in perceived cognitive impairment and cognition-related effects on quality of life relative to EDC (with small to medium effect sizes; Cohen's d = 0.46 and 0.76), whereas no results were found for cognitive performance measures. Relative to EDC, TCE did not exact changes in depression, anxiety, and insomnia symptom severity; however, SQG showed decreases in depression and insomnia symptom severity (with corresponding small to medium effect sizes, Cohen's d = −0.36 and −0.56).

Discussion

Findings from the current exploratory study suggest that low-exertion, gentle exercise, with or without breath and meditative focus, may improve perceived cognitive function, and, that without breath and meditative focus, may improve depressed mood and insomnia symptoms among older BCS. These promising findings may have immediate and long-term implications on accessible treatment options recommended by geriatricians and oncologists treating older BCS at an elevated risk for cognitive impairment.

Clinical trial registration

The parent study was registered on ClinicalTrials.gov, identifier: NCT02690116.
Safety and efficacy of aspirin and indobufen in the treatment of coronary heart disease: a systematic review and meta-analysis
Authors: Zhang Xiaochen , Yan Qiaoyan , Jiang Jiao , Luo Hua , Ren Yu
Published: August 2024

Purpose

This meta-analysis aimed to compare the safety and efficacy of aspirin and indobufen in patients with coronary heart disease. The primary focus was on the incidence of cardiovascular events, bleeding events, and gastrointestinal reactions. Given the relatively limited research on indobufen, this study utilized aspirin as a control drug and employed meta-analysis to integrate existing clinical studies. The goal was to provide a reference for the clinical use of indobufen and to suggest directions for further largescale, multicenter prospective studies.

Methods

This review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. We conducted a comprehensive search of the PubMed, EMBASE, WOS, and Cochrane Library databases to identify all relevant literature on indobufen. A total of nine trials met the inclusion criteria, encompassing seven randomized controlled trails (RCTs) and two retrospective studies. Categorical variables were analyzed using odds ratio and random effects models.

Results

The meta-analysis included nine trials, comprising seven RCTs and two retrospective studies. The pooled results indicated that indobufen significantly reduced the incidence of minor bleeding events, and gastrointestinal discomfort compared to aspirin. However, both drugs had similar effects on the incidence of recurrent angina pectoris, myocardial infarction and mortality due to coronary heart disease.

Conclusion

Indobufen was associated with fewer gastrointestinal reactions and a low risk of bleeding, making it a viable option for patients with high-risk factors for bleeding and gastric ulcers. Despite this, indobufen's short history and limited evidence base compared to aspirin highlight the need for further research. Aspirin remains widely available, cost-effective, and the preferred drug for the primary and secondary prevention of cardiovascular and cerebrovascular diseases. Indobufen or other antiplatelet agents should only be considered when aspirin is not tolerated or contraindicated. Further clinical trials are necessary to determine whether indobufen can replace aspirin.

Systematic Review Registration

https://www.crd.york.ac.uk/, identifier [CRD42024523477].
Tectonic evolution of the Northeastern Paleo-Tethys Ocean during the late Triassic: insights from depositional environment and provenance of the Xujiahe formation
Authors: Xie Fenquan , Xiao Wenzhou , Sami Mabrouk , Sanislav Ioan V. , Ahmed Mohamed S. , Zhang Chenguang , Wang Yongkui , Yan Bingpeng , Hu Bing , Li Ning , Fathy Douaa
Published: August 2024

The Triassic tectonic evolution and affinity among the Yangtze Block, Qinling Orogenic Belt and Songpan-Ganzi Terrane remain subjects of ongoing scientific debate. In this context, the sedimentary rocks of Xujiahe Formation (second segment) (T3x2) represent an ideal case for addressing this issue. In this study, new stratigraphic, geochemical and detrital zircon analyses have been conducted on two sections of the T3x2. The stratigraphic assemblage and features suggests a braided delta as the depositional setting. Whole-rock geochemical results indicate that the sandstones from the T3x2 exhibit moderate palaeo-weathering and primarily originate from upper crustal felsic rocks, mostly S-type granites. The detrital zircons within these sandstones display distinctive age peaks at intervals of 200–300 Ma, 700–900 Ma, and 1800–2000 Ma, similar to those from the Qinling Orogenic Belt. Both the clastic particle composition and whole-rock geochemistry indicates that the T3x2 sections were deposited in a tectonic environment transitioning from a passive to an active continental margin, highlighted by a braided delta influenced by rivers flowing from the northeast to the southwest.
Framework for optimal energy storage duration for maximum-reliability renewable electricity
Authors: Sadhukhan Jhuma , Sen Sohum , Randriamahefasoa T. M. S.
Published: August 2024

Energy system optimization is needed for optimal sustainable net-zero electricity (NZE) mix even at regional/local scales because of the energy storage needs for addressing the intermittency of renewable energy supply. This study presents a novel regional/local energy planning model for optimum sustainable NZE mix under spatiotemporal climate/meteorological and electrical load demand constraints. A generic robust non-linear constrained mathematical programming (NLP) algorithm has been developed for energy system optimization; it minimizes the levelized cost and greenhouse gas emissions while maximizing reliability against stored energy discharge analysis (RADA). Reliability, defined as the ratio of excess stored renewable power discharge to unmet load demand, is a measure of the extent of unmet load demand met by the excess stored renewable power. Coupled with the NLP, the RADA and energy storage evaluations are used to determine the seasonal energy storage (SES) conditions and realistic renewable proportions for NZE. The significance of the proposed framework lies in determining the maximum hours of viable electrical energy storage beyond which the reliability enhancement is infinitesimal. The significant observations of this work include 96 h of maximum viable electrical energy storage beyond which the reliability enhancement is infinitesimal. While this observation is robust based on previous reports for the case of the United States, a realistic NZE mix for Southern United Kingdom is obtained as follows. Direct wind and solar sources can meet 63%, 62%, and 55% of the electricity demands in the southwest, Greater London, and southeast regions of the United Kingdom, respectively; further, battery energy storage systems can increase the renewable proportions by 21%, 22%, and 13% in these three regions. The unmet demands can be met by renewable electricity through SES. Compressed air energy storage (CAES) and pumped hydro storage offer viable SES. Following these, natural gas with carbon capture and storage (CCS), bioenergy, and hydrogen SES are the choices based on increasing cost per lifecycle climate impact potential to meet the electricity demands.
SAFE-MIL: a statistically interpretable framework for screening potential targeted therapy patients based on risk estimation
Authors: Guan Yanfang , Xue Zhengfa , Wang Jiayin , Ai Xinghao , Chen Rongrong , Yi Xin , Lu Shun , Liu Yuqian
Published: August 2024

Patients with the target gene mutation frequently derive significant clinical benefits from target therapy. However, differences in the abundance level of mutations among patients resulted in varying survival benefits, even among patients with the same target gene mutations. Currently, there is a lack of rational and interpretable models to assess the risk of treatment failure. In this study, we investigated the underlying coupled factors contributing to variations in medication sensitivity and established a statistically interpretable framework, named SAFE-MIL, for risk estimation. We first constructed an effectiveness label for each patient from the perspective of exploring the optimal grouping of patients’ positive judgment values and sampled patients into 600 and 1,000 groups, respectively, based on multi-instance learning (MIL). A novel and interpretable loss function was further designed based on the Hosmer-Lemeshow test for this framework. By integrating multi-instance learning with the Hosmer-Lemeshow test, SAFE-MIL is capable of accurately estimating the risk of drug treatment failure across diverse patient cohorts and providing the optimal threshold for assessing the risk stratification simultaneously. We conducted a comprehensive case study involving 457 non-small cell lung cancer patients with EGFR mutations treated with EGFR tyrosine kinase inhibitors. Results demonstrate that SAFE-MIL outperforms traditional regression methods with higher accuracy and can accurately assess patients’ risk stratification. This underscores its ability to accurately capture inter-patient variability in risk while providing statistical interpretability. SAFE-MIL is able to effectively guide clinical decision-making regarding the use of drugs in targeted therapy and provides an interpretable computational framework for other patient stratification problems. The SAFE-MIL framework has proven its effectiveness in capturing inter-patient variability in risk and providing statistical interpretability. It outperforms traditional regression methods and can effectively guide clinical decision-making in the use of drugs for targeted therapy. SAFE-MIL offers a valuable interpretable computational framework that can be applied to other patient stratification problems, enhancing the precision of risk assessment in personalized medicine. The source code for SAFE-MIL is available for further exploration and application at https://github.com/Nevermore233/SAFE-MIL.
Identification and validation of Rab GTPases RAB13 as biomarkers for peritoneal metastasis and immune cell infiltration in colorectal cancer patients
Authors: Lv Fei , Li Xiaoqi , Wang Zhe , Wang Xiaobo , Liu Jing
Published: August 2024

Background

As one of the most common cancer, colorectal cancer (CRC) is with high morbidity and mortality. Peritoneal metastasis (PM) is a fatal state of CRC, and few patients may benefit from traditional therapies. There is a complex interaction between PM and immune cell infiltration. Therefore, we aimed to determine biomarkers associated with colorectal cancer peritoneal metastasis (CRCPM) and their relationship with immune cell infiltration.

Methods

By informatic analysis, differently expressed genes (DEGs) were selected and hub genes were screened out. RAB13, one of the hub genes, was identificated from public databases and validated in CRC tissues. The ESTIMATE, CEBERSORT and TIMER algorithms were applied to analyze the correlation between RAB13 and immune infiltration in CRC. RAB13’s expression in different cells were analyzed at the single-cell level in scRNA-Seq. The Gene Set Enrichment Analysis (GSEA) was performed for RAB13 enrichment and further confirmed. Using oncoPredict algorithm, RAB13’s impact on drug sensitivity was evaluated.

Results

High RAB13 expression was identified in public databases and led to a poor prognosis. RAB13 was found to be positively correlated with the macrophages and other immune cells infiltration and from scRNA-Seq, RAB13 was found to be located in CRC cells and macrophages. GSEA revealed that high RAB13 expression enriched in a various of biological signaling, and oncoPredict algorithm showed that RAB13 expression was correlated with paclitaxel sensitivity.

Conclusion

Our study indicated clinical role of RAB13 in CRC-PM, suggesting its potential as a therapeutic target in the future.
Density of tertiary lymphoid structures and their correlation with prognosis in non-small cell lung cancer
Authors: Xin Shuyue , Wen Shuang , He Peipei , Zhao Yulong , Zhao Hui
Published: August 2024

Background

Tertiary lymphoid structures (TLS), ordered structure of tumor-infiltrating immune cells in tumor immune microenvironment (TIME), play an important role in the development and anti-tumor immunity of various cancers, including liver, colon, and gastric cancers. Previous studies have demonstrated that the presence of TLS in intra-tumoral (IT), invasive margin (IM), and peri-tumoral (PT) regions of the tumors at various maturity statuses. However, the density of TLS in different regions of non-small cell lung cancer (NSCLC) has not been extensively studied.

Methods

TLS and tumor-infiltrating immune cells were assessed using immunohistochemistry (IHC) staining in 82 NSCLC patients. Tumor samples were divided into three subregions as IT, IM and PT regions, and TLS were identified as early/primary TLS (E-TLS) or secondary/follicular TLS (F-TLS). The distribution of TLS in different maturity statuses, along with their correlation with clinicopathological characteristics and prognostic value, was assessed. Nomograms were used to predict the probability of 1-, 3-, and 5-year overall survival (OS) in patients with NSCLC.

Results

The density of TLS and proportion of F-TLS in the IT region (90.2%, 0.45/mm2, and 61.0%, respectively) were significantly higher than those in the IM region (72.0%, 0.18/mm2, and 39.0%, respectively) and PT region (67.1%, 0.16/mm2, and 40.2%, respectively). A lower density of TLS, especially E-TLS in the IM region, was correlated with better prognosis in NSCLC patients. CD20+ B cells, CD3+ T cells, CD8+ cytotoxic T cells, and CD68+ macrophages were significantly overexpressed in the IM region. CD20+ B cells and CD3+ T cells in the IM region were significantly correlated with the density of E-TLS, while no statistically significant correlation was found with F-TLS. The E-TLS density in the IM region and TNM stage were independent prognostic factors for NSCLC patients. The nomogram showed good prognostic ability.

Conclusions

A higher density of E-TLS in the IM region was associated with a worse prognosis in NSCLC patients, potentially due to the inhibition of TLS maturation caused by the increased density of suppressive immune cells at the tumor invasion front.
Application of hydrogels in cancer immunotherapy: a bibliometric analysis
Authors: Liu Xiang , Zhou Qiang , Yang Yue , Chen Erhua
Published: August 2024

Background

Cancer immunotherapy has made significant progress in recent years, with numerous studies worldwide. Immunotherapy has had a transformative impact on oncology and autoimmune diseases. In the biomedical arena, hydrogels with good properties are widely used in cancer immunotherapy. Our study used bibliometrics to analyze the changing trends in using hydrogels for cancer immunotherapy.

Methods

From 2013 to 2023, a systematic search was conducted in the Web of Science Core Collection database to identify reviews and articles discussing the applications of hydrogels in cancer immunotherapy. The software CiteSpace was used to visually perform the bibliometric analysis in terms of research trends, countries, institutions, authors, journals, and keywords. Individual authors’ productivity was assessed with the Lotka’s law. The most relevant publication sources were identified by Bradford’s law.

Results

A total of 422 English-language publications related to hydrogels in cancer immunotherapy were collected. The number of annual publications increased rapidly after 2021 and remained constant for the past two years. China published the most articles in this field. The institution with the maximum number of published articles was the Chinese Academy of Sciences in China. Chen. Q was the most prolific author, and Liu. Z was the second most published author. In terms of journal contributions, the journal “Biomaterials” had the highest number of publications (n = 30). Biomaterials, Advanced Functional Materials and Journal of Controlled Release were the most influential journals. Keyword analysis revealed that cancer immunotherapy, drug delivery, immunogenic cell death, tumor microenvironment, injectable hydrogels, and immune checkpoint blockade were the primary research hotspots. In recent 3 years, adoptive T-cell therapy, black phosphorus, cell capture, adaptive cell therapy, tumor microenvironment, photodynamic therapy, and sustained release were the research hotspots in this field. Our study summarizes the objective of hydrogels in cancer immunotherapy in recent years, providing a reference for potential researchers in related field.

Conclusion

This bibliometric analysis shows the progress and trend of research on hydrogels in cancer immunotherapy. This study provides a significant avenue for future investigation into current concerns and trends in research within this field.
The effect of second language acquisition on central auditory processing abilities and its interaction with HIV
Authors: Kambhampaty Abby , Niemczak Christopher E. , Leigh Samantha M. , Lichtenstein Jonathan , Adhikari Monika , Fellows Abigail M. , Magohe Albert , Gui Jiang , Zhang Linda , Massawe Enica R. , Buckey Jay C.
Published: August 2024

Introduction

Second language learning is a multifaceted task that benefits across numerous neurocognitive domains including central auditory processing. Existing cross-sectional and longitudinal data show that performance on tests of central auditory processing [central auditory tests (CATs)] worsens with HIV infection. Second language learning may modify this relationship. To explore the relationship between second language learning, central auditory processing, and its interaction with HIV, we assessed the effect of learning English as a second language on CATs among children both living with and without HIV (CLWH/CLWOH) in Dar es Salaam, Tanzania.

Methods

Three hundred and seventy-two native Kiswahili speaking children aged 3-10 years old (196 CLWOH, 176 CLWH) were enrolled. Participants completed questionnaires about English language learning, socioeconomic status (SES), and health history. Three central auditory tests-the Triple Digit Test (TDT), the Staggered Spondaic Word Test (SSW), and the Hearing-In-Noise Test (HINT)-were used to assess each participant's central auditory processing abilities. Multivariate linear regression was used to assess the effect of written and spoken English language learning at home and in school on CATs with age, HIV-status, and SES included in each model.

Results

HIV status, age, and SES were all significant predictors of all three central auditory tests, with CLWH performing significantly worse on all three CATs than CLWOH. Children actively learning spoken and written English at home had significantly better central auditory processing abilities on the TDT compared to children not actively learning English at home (p < 0.01) independent of HIV status, age, and SES. Children learning spoken and written English at school performed significantly better on the HINT (p < 0.05) than those not actively learning English at school.

Discussion

Learning English at home and learning English in school were associated with improved central auditory performance independent of HIV status, SES, and age. These findings also underscore the significance of second language acquisition as a potential mechanism of improving central auditory function within a Kiswahili-speaking cohort. This study found differences in central auditory processing between children exposed to English at home and in school, suggesting differences in language learning in both settings mediated by SES, and this benefit exists regardless of HIV status.
The radiological diagnosis of pregnancy associated venous thromboembolism: a review of current research
Authors: Yang Di , Wang Li
Published: August 2024

One of the main causes of unfavorable pregnancy outcomes in expectant mothers is pregnancy-associated venous thromboembolism. Although pregnancy-related venous thromboembolism does not always manifest obvious clinical symptoms and lacks a comprehensive standard risk assessment and prediction system as well as simple and effective laboratory testing techniques, timely and accurate diagnosis can still help reduce the probability of adverse pregnancy outcomes. To aid in the early detection, diagnosis, and treatment of pregnancy- associated venous thromboembolism, we attempt to provide an overview of the radiological diagnostic techniques for various forms of the condition.
Imaging findings of Budd-Chiari syndrome caused by intravenous leiomyomatosis: a case report
Authors: Wang Yahong , Dong Yifan , Tian Xueyu , Chen Yu , Yang Xiao , Li Jianchu
Published: August 2024

Intravenous leiomyomatosis (IVL) is a rare gynecological-related tumor. It can invade and extend in the blood vessel and eventually involve the cardiac cavity or even the pulmonary artery. IVL generally does not adhere to the vein wall and infrequently leads to the manifestation of Budd-Chiari syndrome (BCS). In this case report, the presence of a sizable tumor obstructed the second hepatic portal, impeding the return flow of the hepatic veins, thereby precipitating the development of BCS. The presence of collateral veins and dilation of the accessory hepatic vein were identified through computed tomography venography and ultrasonography, thus supporting the diagnosis of BCS. The patient underwent a comprehensive surgical resection and was found to have a favorable prognosis.
Transcriptional regulator MarT negatively regulates MarT-regulated motility gene I, a new gene involved in invasion and virulence of Salmonella enterica
Authors: Jerez Sebastián A. , Mora Aracely Y. , Millanao Ana R. , Saavedra Claudia P. , Bucarey Sergio A. , Mora Guido C. , Villagra Nicolás A. , Hidalgo Alejandro A.
Published: August 2024

The speciation of Salmonella occurred by acquisition of genomic islands from other bacterial species and continued to diverge into subspecies and serovars with diferent range of host. S. enterica serovar Typhimurium (STM) is a generalist pathogen infecting hosts that include birds, mice, and humans, whilst S. enterica serovar Typhi (STY) is a restricted-host pathogen, infecting only humans. Despite their ranges of hosts, STM and STY possess 97–98% identity. Gain of genes by horizontal transference and loss of genes by mutations, are believed essential for differentiation of Salmonella. Salmonella pathogenicity island 3 (SPI-3) is an example combining these two processes. SPI-3 encodes misL and marT, among other genes. In STM, misL is required for gut colonization. Furthermore, protein MarT, positively regulates expression of misL by binding to misL-promoter. On the other hand, in SPI-3 of STY, marT and misL are pseudogenes. Interestingly, the gene t3766 (gene involved in resistance to H2O2) is present only in STY and is negatively regulated when marTSTM is heterologously expressed in STY. Based on the view that MarT might regulate genes implicated in virulence, this work searched for new genes regulated by MarT. In silico searches for possible MarT target genes were performed, and 4 genes were selected for further analysis as they contained at least 2 copies of the consensus MarT-binding sequence in their promoters. Mutating marT in STM or heterologously expressing marTSTM in STY confirmed that MarT negatively regulates ORF STY1408 or STM14_2003, its homologue in STM. STY1408 encodes for a putative protein with homology to methyl accepting chemotaxis proteins, which participate in chemotaxis and motility. Therefore, STY1408 was named mrmI (MarT-regulated motility gene I). Motility assays confirmed that the product of mrmI modulates motility. In addition, in vitro infection of cells with STM and STY mutants in mrmI reduces association with cells at 1, 3 and 24 h post-infection. Oral infection of mice showed that a mrmI null mutant was defective in producing systemic disease. Therefore, we conclude that MarT regulated mrmI, is involved in virulence of Salmonella. While pseudogenization of marT might modulate the fitness of narrow host range STY.
Leptospiral adhesins: from identification to future perspectives
Authors: Surdel Matthew C. , Coburn Jenifer
Published: August 2024

Leptospirosis is a significant zoonosis worldwide, with disease severity ranging from a mild non-specific illness to multi-organ dysfunction and hemorrhage. The disease is caused by pathogenic bacteria of the genus Leptospira, which are classified into pathogenic and saprophytic clades. Bacterial binding to host molecules and cells, coordinated by adhesin proteins, is an important step in pathogenesis. While many leptospiral adhesins have been identified, the vast majority have not been characterized in vivo. Herein, we present an overview of the current methodologies and successes in identifying adhesins in Leptospira, including known biological roles in vivo. We will also identify and discuss potential areas for future research.
Breathomics for diagnosing tuberculosis in diabetes mellitus patients
Authors: Xu Rong , Zhang Ying , Li Zhaodong , He Mingjie , Lu Hailin , Liu Guizhen , Yang Min , Fu Liang , Chen Xinchun , Deng Guofang , Wang Wenfei
Published: August 2024

Introduction

Individuals with diabetes mellitus (DM) are at an increased risk of Mycobacterium tuberculosis (Mtb) infection and progressing from latent tuberculosis (TB) infection to active tuberculosis disease. TB in the DM population is more likely to go undiagnosed due to smear-negative results.

Methods

Exhaled breath samples were collected and analyzed using high-pressure photon ionization time-of-flight mass spectrometry. An eXtreme Gradient Boosting (XGBoost) model was utilized for breathomics analysis and TB detection.

Results

XGBoost model achieved a sensitivity of 88.5%, specificity of 100%, accuracy of 90.2%, and an area under the curve (AUC) of 98.8%. The most significant feature across the entire set was m106, which demonstrated a sensitivity of 93%, specificity of 100%, and an AUC of 99.7%.

Discussion

The breathomics-based TB detection method utilizing m106 exhibited high sensitivity and specificity potentially beneficial for clinical TB screening and diagnosis in individuals with diabetes.
Effect of hypoglycemic events on cognitive function in individuals with type 2 diabetes mellitus: a dose–response meta-analysis
Authors: Ye Min , Yang Qiqi , Zhang Lele , Song Hudie , Fu Qin , Qian Jun , Xie Hongyu , Yuan Aihong
Published: August 2024

Background

Type 2 diabetes mellitus (T2DM) is widely acknowledged as a vital warning sign contributing to cognitive dysfunction. However, there is still a lack of consensus on whether hypoglycemic events resulting from poor glycemic control increase the risk of cognitive dysfunction in people with diabetes, and the potential dose–response correlation between hypoglycemic events and cognitive dysfunction remains unexplored. The primary objective of the current study was to assess the contribution of hypoglycemic events to cognitive dysfunction in T2DM patients and the dose–response correlation between the two.

Methods

A comprehensive search of nine major databases was executed from inception to May 2023. We screened all observational studies examining the connection between hypoglycemia and cognitive dysfunction. The DerSimonian-Laird method was used to compute the combined risk ratio (RR) and its 95% confidence interval (CI). Additionally, dose–response analysis was employed to investigate the correlation between the frequency of hypoglycemia and the likelihood of cognitive dysfunction.

Results

A total of 30 studies of different levels in 17 articles with 3,961,352 participants were included in this review. The pooled RR for the connection of hypoglycemia and the likelihood of cognitive dysfunction was 1.47 (95% CI: 1.35–1.60). Subgroup analyses showed that the pooled RR for the likelihood of cognitive dysfunction was 1.20 (95% CI: 1.11–1.31) for one episode of hypoglycemia, 1.41 (95% CI: 1.05–1.88) for two episodes of hypoglycemia, and 1.62 (95% CI: 1.20–2.91) for three or more episodes of hypoglycemia. Dose–response analysis showed a linear dose–response relationship between hypoglycemia and the likelihood of cognitive dysfunction (exp (b) = 1.178694, z = 7.12, p < 0.001).

Conclusion

Our investigations demonstrated a 47% heightened likelihood of cognitive dysfunction in individuals with hypoglycemia compared to those without. Furthermore, the likelihood of cognitive dysfunction climbed by 17.87% for every subsequent episode of hypoglycemia. Therefore, long-term monitoring of blood glucose, periodic screening of cognitive function, and moderate health education should be encouraged, which will be beneficial for people with diabetes to prevent hypoglycemic events and cognitive dysfunction.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, CRD42023432352.
Global, regional, and national time trends in the burden of epilepsy, 1990–2019: an age-period-cohort analysis for the global burden of disease 2019 study
Authors: Shan Tao , Zhu Yahui , Fan Haozhi , Liu Zeye , Xie Jing , Li Mao , Jing Shenqi
Published: August 2024

Background

Epilepsy is a non-communicable chronic brain disease that affects all age groups. There are approximately 50 million epilepsy patients worldwide, which is one of the most common neurological disorder. This study reports the time trends in the burden of epilepsy from 1999 to 2019.

Methods

We evaluated the disease burden and its temporal trends of epilepsy using the prevalence and years lived with disability (YLDs), which was estimated based on the Global Burden of Disease (GBD) 2019 study. The age-period-cohort (APC) model was used to estimate the temporal trends of the epilepsy prevalence and YLDs rates, and to analyze the relative risks of age, periods and queues (age/period/queue effects).

Results

In the past 30 years, the global age-standardized prevalence rate and age-standardized rate has increased by 29.61% and 27.02%, respectively. Globally, the APC model estimated the net drift of prevalence and YLDs were 0.88% (95% CI: 0.83–0.93) and 0.80% (95% CI: 0.75–0.85) per year. Among 204 countries and territories, the YLDs in 146 and prevalence 164 showed an increasing trend. And the risk of YLDs and prevalence increases with age, with the lowest risk among 0–4 years old and the highest risk among 75–79 years old. Unfavorable increasing period and cohort risks of YLDs and prevalence were observed.

Conclusion

Over the past 30 years, the YLDs and prevalence of epilepsy have gradually increased globally and unfavorable increasing period and cohort risks were observed. Emphasizing epilepsy prevention, strengthening epilepsy health education, optimizing older adults epilepsy diagnosis and treatment plans, and actively promoting epilepsy diagnosis and treatment plans can effectively reduce new cases of epilepsy and related disabilities.