bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Problem-Based Learning Model Accompanied by the QuizWhizzer Game on Critical Thinking Skills and Physics Learning Outcomes of Vocational Students
Authors: Nurroniah Zhahrotun , Subiki Subiki , Nuraini Lailatul
Published: August 2024

Physics learning is learning that influences students' thinking skills so that students can be skilled in the cognitive and psychomotor fields and support students to think systematically, creatively, and objectively. But in fact, the implementation of learning that occurs is not contextual because the way teachers teach in class is still boring and less varied, so student involvement in the learning process in class becomes less active, both asking and answering questions. This study aims to determine the effect of using a problem-based learning model accompanied by a QuizWhizzer game on vocational students' critical thinking skills and physics learning outcomes in Jember Regency. This study used a quantitative approach, specifically a true experimental design of a posttest-only control group. The study population consisted of class X students at SMK Negeri 1 Jember, with the sample comprising two classes selected from all classes at SMK Negeri 1 Jember. Data analysis of students' critical thinking skills using the Mann-Whitney U Test resulted in a significance value (2-tailed) of 0.038 < 0.05, which leads to the rejection of H0 and acceptance of Ha. Data analysis of students' physics learning outcomes using the Mann-Whitney U Test and hypothesis testing results resulted in a significance value (2-tailed) of 0.023 < 0.05, which leads to the rejection of H0 and acceptance of Ha. These findings indicate a significant effect of applying a problem-based learning model assisted by QuizWhizzer on students' critical thinking skills and physics learning outcomes, especially on energy and its changes. With this research, teachers can design more effective learning models to support critical thinking skills and optimal student learning outcomes in physics learning accompanied by learning media by analysing student needs, selecting learning models, developing learning media, planning learning activities, and implementing them.
Arquitetura e percepção ambiental ou uma construção fenomenológica do habitar
Authors: Laelia Regina Batista Nogueira
Published: August 2024

Este estudo objetiva evidenciar as relações espaciais do povo Sateré-Mawé, fundamentado em um relato de vivência após dias de imersão nesse grupo. A abordagem fenomenológica foi adotada como referencial teórico. O relato em questão busca compreender a percepção ambiental de um grupo focal desse povo, especialmente durante a proposta inicial de construir uma casa de parto em Maués, Amazonas, que acolhesse as mulheres Sateré quando estas estivessem na cidade. Utilizando dinâmicas em grupo e metodologias participativas de projeto de arquitetura, além de mutirões de capina e abertura de clareiras, o estudo demonstra as possibilidades de uma arquitetura fenomenológica. Essa abordagem se baseia no conhecimento tradicional e na percepção ambiental dos povos tradicionais, elementos essenciais para o desenvolvimento de espaços que se alinham à arquitetura vernacular. A fenomenologia, como principal suporte teórico, permite a integração entre campo e teoria, abrindo caminhos para novas possibilidades e compreensões a partir das experiências do mundo vivido, enfocando principalmente nas relações sensoriais e de percepção.
Evaluation of Instagram’s popularity: Brand cue and message appeal as explanatory variables
Authors: Anggraini Putri , Gunarto Muji
Published: August 2024

The internet’s ascent has transformed digital marketing, with social media platforms like Instagram becoming essential tools. The “Instagrammable” trend has gained traction in the food industry, using visual appeal to drive business success and expand markets. This research investigates the factors influencing Instagram’s popularity metrics in the food sector, focusing on South Sumatra. A survey of 250 Instagram users was conducted using accidental sampling. The study assessed various factors on a Likert scale from 1 (strongly disagree) to 7 (strongly agree). The findings revealed that brand cue positively impacts selling strategy and information search. Additionally, message appeal positively influences selling strategy and information search but negatively correlates with the popularity of social media messages, suggesting that specific appeals might hinder social media popularity. Conversely, selling strategy and information search positively affect the popularity of social media messages, highlighting the importance of engaging consumers and providing informative content for brand popularity on social platforms. While brand cues do not directly influence the popularity of social media messages, message appeal and brand cues are crucial in shaping consumer interactions on social media. These results offer practical insights into utilizing brand cues and message appeal to cultivate consumer relationships, differentiate brands, and influence purchasing behavior through social media. The study provides valuable guidance for businesses navigating the complex world of social media marketing, emphasizing the importance of tailored messaging and brand presence in driving consumer engagement and brand perception within the competitive food industry.
Identificación y evaluación de riesgos ambientales en un astillero: enfoque en fuentes de riesgo y sustancias potencialmente peligrosas
Authors: Durán Joaquín
Published: August 2024

Se propone el uso del análisis de riesgos ambientales acorde con la norma UNE 150008:2008 para mejorar el desempeño ambiental de un astillero y prevenir la contaminación. La metodología empleada incluye la identificación de fuentes de riesgo, sustancias potencialmente peligrosas y escenarios de causas y consecuencias. La evaluación resultante se utiliza para cuantificar y clasificar los riesgos asociados a las actividades del astillero, lo que permite priorizar los esfuerzos y establecer objetivos ambientales.
A Metagenomics Pipeline to Characterize Self-Collected Vaginal Microbiome Samples
Authors: Thomas-White Krystal , Hilt Evann E , Olmschenk Genevieve , Gong Maryann , Philips Caleb , Jarvis Courtney , Sanford Nicholas , White Jennifer , Navarro Pita
Published: August 2024

Vaginitis is a widespread issue for women worldwide, yet current diagnostic tools are lacking. Bacterial vaginosis (BV) is the most prevalent type of vaginitis, found in 10-50% of reproductive aged women. Current diagnostic methods for BV rely on clinical criteria, microscopy, or detection of a few microbes by qPCR. However, many vaginal infections lack a single etiological agent and are characterized by changes in the vaginal microbiome community structure (e.g. BV is defined as a loss of protective lactobacilli resulting in an overgrowth of anaerobic bacteria). Shotgun metagenomic sequencing provides a comprehensive view of all the organisms present in the vaginal microbiome (VMB) allowing for a better understanding of all potential etiologies. Here, we describe a robust VMB metagenomics sequencing test with a sensitivity of 93.1%, specificity of 90%, a negative predictive value of 93.4%, and a positive predictive value of 89.6% certified by Clinical Laboratory Improvement Amendments (CLIA), the College of American Pathologist (CAP) and the Clinical Laboratory Evaluation Program (CLEP). We sequenced over 7,000 human vaginal samples with this pipeline and described general findings and comparison to US census data.
Conceptual Design of Wood Pillar Foundation in Swampy Soil A Construction Solution for Drainage Improvement with Adaptation of Rain Garden’s Concept
Authors: Agusniansyah Nursyarif , Syarief Akhmad , Noor Rijali , Annisa Nova
Published: August 2024

Building on swampy land is a major challenge due to the soft and waterlogged soil properties. Traditional approaches using wooden pile foundations have long been used but new technology with a more environmentally friendly and efficient approach needs to be applied to swampy land. The objectives of this research are: to obtain a conceptual model of modified wooden pile foundation construction combined with concrete, to identify the impact of soil condition changes due to foundation implementation and the swampy groundwater environment, and to determine the application of the 'constructed wetland' and rain garden adaptation methods to the foundation model concept.In this research, a qualitative method was used with a descriptive approach based on literature studies and data collection through surveys/field observations. There is interdisciplinary collaboration from architectural, material, and environmental fields. The method conducted includes experimental testing of the foundation model concept. The research stages are: identifying variations in foundation design on wetland, survey/field study observation of foundations in Banjarmasin, architectural-material-environmental analysis of the foundation model concept with rain garden adaptation, and finally, conclusions and suggestions.The rain garden concept is an innovation that can help address waterlogging issues by enhancing the improvement of foundation construction and drainage in swampy soils. In this paper, the use of galam wood, wooden pile foundations, and construction technology innovations to improve swampy soil conditions are discussed in detail.
Waste Management and Technological Advancement: Insights from Europe
Authors: Laureti Lucio , Costantiello Alberto , Anobile Fabio , Leogrande Angelo , Magazzino Cosimo
Published: August 2024

The paper analyzes the relationship between urban waste recycling and technological innovation systems in Europe. Data from the Global Innovation Index for 34 European countries in the period 2013-2022 was used. To analyze the characteristics of European countries in terms of waste recy-cling capacity, the k-Means algorithm optimized with the Elbow method and the Silhouette Coef-ficient was used. The results show that the optimal number of clusters is 3. Panel data results show that waste recycling increases with domestic market scale, gross capital formation, and the diffu-sion of Information and Communication Technologies (ICTs), while it decreases with the infra-structure index, business sophistication index, and the average expenditure in research and devel-opment of large companies.
How Is Tourism Geography Situated in Taiwan? Geography versus Tourism and Recreation
Authors: Jiang Quan yue , Fang Wei-Ta Fang , LePage Ben , Han Guo-sheng
Published: August 2024

Tourism Geographers in Anglo-American geographical departments migrate to teach and research tourism in applied schools and departments such as business, resource and environment, and even cultural and creative industry. Under national research assessment and funding pressures, tourism geographers are not encouraged to keep on undertaking serious theoretical researches related to their mother discipline and transferred to research applied researches and publish in hybrid journals. Such changes in tourism geographers` research behavior have not been tested and reported in Asian academic contexts, especially Taiwan while frequently being discussed internationally. This research aims to investigate the differences in the research behavior of tourism geographers between those who are staffed in geography departments and those who migrate to applies departments such as tourism and recreation, resource and environment, and others in terms of publishing journals, paradigmatic approaches, research types(qualitative, quantitative, and mixed), research methods and geographical scales conducted based on content analysis of articles published by tourism geographers in geography and tourism and recreation departments. This study revealed that there are significant differences in the research behavior of tourism geographers between different department. Finally, this paper explains the differences in terms of Taiwan changing social and economic conditions, academic traditions and politics of geography and applied departments, research habits and preferences of tourism geographers among departments and other aspects. This study can help international tourism geography academic circles to understand the complexity and diversity of tourism geography academic landscape of the Chinese language.
Salmonella Detection in Food Using a HEK-hTLR5 Reporter Cell-based Sensor
Authors: Eser Esma , Felton Victoria A. A , Drolia Rishi , Bhunia Arun K
Published: August 2024

The development of a rapid, sensitive, specific method for detecting foodborne pathogens is paramount in supplying safe food to enhance public health safety. Despite the significant improvement in pathogen detection methods, key issues are still associated with rapid methods, such as distinguishing living cells from dead, pathogenic potential or health risk of the analyte at the time of consumption, detection limit, and sample-to-result. The mammalian cell-based assays analyze pathogens' interaction with the host cells and are responsive to only live pathogens or active toxins. In this study, a human embryonic kidney (HEK293) cell line expressing Toll-Like Receptor 5 (TLR-5) and chromogenic reporter system (HEK dual hTLR5) was used for the detection of viable Salmonella in a 96-well tissue culture plate. This cell line responds to low concentrations of TLR5 agonist flagellin. Stimulation of TLR5 ligand activates nuclear factor-kB (NF-κB) - linked alkaline phosphatase (AP-1) signaling cascade inducing the production of secreted embryonic alkaline phosphatase (SEAP). With the addition of a ρ-nitrophenyl phosphate as a substrate, a colored end product representing a positive signal is quantified. The assay's specificity was validated with the top 20 Salmonella enterica serovars and 19 non-Salmonella spp. The performance of the assay was also validated with spiked food samples. The total detection time (sample-to-result), including shortened pre-enrichment (4 h) and selective enrichment (4 h) steps with artificially inoculated outbreak-implicated food samples (chicken, peanut kernel, peanut butter, black pepper, mayonnaise, and peach) was 15 h when inoculated at 1–100 CFU/25 g sample. These results show the potential of HEK-DualTM hTLR5 cell-based functional biosensors for rapid screening of Salmonella.
Determination of Recovery by Healing or Compensation Using Multifrequency Vestibular Tests and Subjective Functional Scales in a Human Model of Vestibular Neuritis
Authors: Armato Enrico , Dumas Georges , Perottino Flavio , Casteran Matthieu , Perrin Philippe
Published: August 2024

Background: Vestibular Neuritis (VN) induces Acute Vestibular Syndrome (AVS). This study aimed to identify predictive factors of VN recovery by healing and/or compensation. Methods: In this longitudinal and prospective study, 40 patients were included. The first assessment (T0) included medical history taking (general and specific), for cardiovascular risk factors (CVRFs), a battery of diagnostic tests, bithermal caloric test (BCT), video Head Impulse Test (VHIT) and skull vibration induced nystagmus (SVIN). All patients also completed a Dizziness Handicap Inventory (DHI). All assessments were repeated 90±15 days (T3) later. Subjective criteria compensation were based on DHI total score and objective criteria compensation on the instrumental test results. Four groups of patients (A, B, C, D) were delineated by combining patients with normal vs abnormal vestibular tests and patients with normal vs abnormal DH. Results: CVRFs were associated with a poorer recovery of symptoms but not the age or BMI. Vestibular tests mainly BCT (canal paresis %), VHIT (lateral semicircular canal gain) and SVINT (nystagmus slow phase velocity) recovered within normal values in 20%, 20%, 26% of cases respectively at T3 for the 40-patient group. Conclusions: Vascular risk factors (hypercholesterolemia) are correlated with patients who do not recover their symptoms either by healing or compensation. The evolution did not show any significant difference between high or low frequency vestibular tests in patients recovering from their symptoms. However, some patients with objective recovery may continue to show persisting subjective symptoms.
Assessment of Menopausal Symptoms Awareness Among Women in Bharatpur, Nepal
Authors: Panta Aliza , Dahal Babita , Koirala Hari
Published: August 2024

Background: Menopause signifies the end of a woman's reproductive phase, characterized by the cessation of ovarian hormone production and the termination of menstrual cycles, typically occurring between the ages of 45 and 55. The transition involves a range of physical and psychological symptoms manifesting before, during, and after menopause. Method: This cross-sectional analytical study assessed the level of awareness regarding menopausal symptoms among women in Bharatpur-9, Chitwan. A non-probability sampling technique was used to select 257 respondents. Data were collected using a semi-structured interview schedule and analyzed with SPSS version 20.0. Results: Among the respondents, 53.3% were aged 40-49 years (Mean ± S.D.; 48.84 ± 6.27), and 69.3% experienced menarche between ages 13- 15. A majority (59.9%) had not yet reached menopause. Knowledge of menopausal symptoms was as follows: joint pain (90.7%), sleep problems (90.7%), irritability (90.7%), hot flushes (22.2%), anxiety (22.2%), sadness (22.2%), vaginal dryness (22.6%), loss of interest in sex (25.7%), palpitations (18.7%), and decreased visual acuity (9.3%). Statistical significance was found between awareness levels and factors such as religion (p=0.00), ethnicity (p=0.00), educational status (p=0.006), family type (p=0.043), cessation of menstruation (p=0.013), and cause of menopause (p=0.00). Overall, 53.7% of respondents had average knowledge about menopausal symptoms. Conclusion: The study concluded that over half of the respondents possessed average awareness of menopausal symptoms. Hence, there is a need for women to receive correct and comprehensive information regarding menopausal symptoms and their management.
Lysine 473 Regulates the Progression of SLC7A11, the Cystine/Glutamate Exchanger, through the Secretory Pathway
Authors: Koppin Anna , Chase Leah
Published: August 2024

System xc-, the cystine/glutamate exchanger, is a membrane transporter that plays a critical role in the antioxidant response of cells. Recent work has shown that System xc- localizes to the plasma membrane during oxidative stress, allowing for increased activity to support production of glutathione. In this study, we used site-directed mutagenesis to examine the role of C-terminal lysine residues (K422, K472, K473) of xCT (SLC7A11) in regulating System xc-. We observed that K473R exhibits loss of transporter activity and membrane localization and is 7.5 kD lower in molecular weight, suggesting that K473 regulates System xc- trafficking and is modified under basal conditions. After ruling out ubiquitination and neddylation, we demonstrated that unlike WT xCT, K473R lacks N- and O-glycosylation and is sequestered in the endoplasmic reticulum. Next we demonstrated that K473Q, a constitutively acetylated lysine mimic, also exhibits loss of transporter activity, decreased membrane expression, and a 4 kD decrease in molecular weight, however, it is N- and O-glycosylated and localized to the endoplasmic reticulum and Golgi. These results suggest that acetylation and deacetylation of K473 in the endoplasmic reticulum and Golgi, respectively, serves to regulate the progression of the transporter through the biosynthetic pathway.
Collaborative Channel Perception of UAV Data Link Network Based on Data Fusion
Authors: Zhao Zhiyong , Mao Zhongyang , Zhang Zhilin , Pan Yaozong , Xu Jianwu
Published: August 2024

The existing collaborative channel perception suffered from unreasonable data fusion weight allocation, which mismatched the channel perception capability of node devices. This often led to significant deviations between the channel perception results and the actual channel state. To solve this issue, this paper integrated the data fusion algorithm from evidence fusion theory with data link channel state perception. It applied the data fusion advantages of evidence fusion theory to evaluate the traffic pulse statistical capability of network node devices. Specifically, the typical characteristic parameters describing the channel perception capability of node devices were regarded as evidence parameter sets under the recognition framework. By calculating the credibility and falsity of the characteristic parameters, the differences and conflicts between nodes were measured to achieve a comprehensive evaluation of the traffic pulse statistical capabilities of node devices. Based on this evaluation, the geometric mean method was adopted to calculate channel state perception weights for each node within a single-hop range, and a weight allocation strategy was formulated to improve the accuracy of channel state perception.
Thermodynamic Factors Controlling Electron Transfer amongst the Terminal Electron Acceptors of Photosystem I: Insight from Kinetic Modelling
Authors: Santabarbara Stefano , Casazza Anna Paola
Published: August 2024

Photosystem I is a key component of primary energy conversion in oxygenic photosynthesis. Electron transfer reactions in Photosystem I take place across two, parallel, electron transfer chains that converge after a few electron transfer steps, sharing both the terminal electron acceptors, that are a series of three Iron-Sulphur (Fe-S) clusters known as , and , and the terminal donor, . The two electron transfer chains show kinetic differences which are, due to their close geometrical symmetry, mainly attributable to the tuning of the physical-chemical reactivity of the bound cofactors, exerted by the protein surroundings. The factors controlling the rate of electron transfer between the terminal Fe-S clusters are still not fully understood because of the difficulties of monitoring these events directly. Here we present a discussion concerning the driving forces associated to electron transfer between and as well as and , employing a tunnelling-based description of the reaction rates coupled to the kinetic modelling of forward and recombination reactions. It is concluded that the reorganisation energy for oxidation shall be lower than 1 eV. Moreover, it is suggested that the analysis of mutants with altered redox properties can also provide useful information concerning the upstream, phylloquinone, cofactor energetics.
New Insights into Chronic Pancreatitis Treatment: Potential Mechanisms and Applications of Probiotics and Prebiotics
Authors: Pan Yingyu , Li Jianing , Fan Zhengyang , Chen Yonghao , Huang Xiaoxuan , Wu Dong
Published: August 2024

Chronic pancreatitis is a progressive fibroinflammatory disorder with no currently satisfactory treatment. Emerging evidence suggests an association between gut microbial dysbiosis and chronic pancreatitis. Although direct causative evidence is lacking, it is hypothesized that the gut microbiota may play a pivotal role in modulating pancreatic function via the gut-pancreas axis. Thus, modulating the gut microbiota through the administration of probiotics or prebiotics may alleviate pancreatic disorders. In this review, we first propose the potential mechanisms by which specific probiotics or prebiotics may ameliorate chronic pancreatitis, including the alleviation of small intestinal bacterial overgrowth (SIBO), facilitation of short-chain fatty acids (SCFAs) production, activation of the glucagon-like peptide 1 receptors (GLP-1Rs) in the pancreas. Since there are currently no probiotics or prebiotics used for the treatment of chronic pancreatitis, we have discussed research in other disease models that use probiotics or prebiotics to modulate pancreatic endocrine and exocrine functions and prevent pancreatic fibrosis. This provides indirect evidence for their potential application in the treatment of chronic pancreatitis. We anticipate that this research will stimulate further investigation into the gut-pancreas axis and the potential therapeutic value of probiotics and prebiotics in chronic pancreatitis.
A Novel Approach for Reducing Catheter Associated Urinary Tract Infections in Intermittent Catheter Users
Authors: Sarangapani Shantha , Hopke Katherine
Published: August 2024

Catheter-associated urinary tract infections (CAUTI) are a recurring problem for individuals with neurogenic bladder who rely on frequent intermittent catheterization. Dysbiosis of the urine microbiome, characterized by the presence of pathogenic organisms and insufficient levels of Lactobacilli, is common in this population. We propose that introducing beneficial Lactobacilli via a catheter lubricant could act as a prophylactic "live barrier" to enhance beneficial bacteria in the urethra. The IC TF1 Catheter Lubricant gel discussed in this study is a freshly prepared viscous gel composed of sterile gelling components and a dry Lactobacillus Rhamnosus spp. powder. The gel prepared on- site by simple mixing, is usable for 24 hours with predictable and precise viability counts. Our investigation focuses on the design, physical attributes, manufacturability with quality control criteria, stability of probiotic viability, antimicrobial properties, cellular toxicity, and tissue compatibility of the IC TF1 gel. The gel demonstrates promising physical properties and biocompatibility, leading to the need for further pre-clinical and human trials to assess its safety and effectiveness in reducing pathogenic urethral colonization.The localized delivery of probiotic supplementation through catheter lubrication has the potential to enhance the well-being of individuals with neurogenic bladder and aid in the management of CAUTI.
MINOCA of Ischemic, Non-atherosclerotic Cause: Pathophysiological Approach of Diagnosis and Prognosis – a Narrative Review
Authors: Khattab Elina , Karelas Dimitrios , Pallas Theofilos , Kostakis Panagiotis , Patsourakos Nikolaos , Papadopoulos Constantinos Hristou , Sideris Skevos , Kadoglou Nikolaos P.E.
Published: August 2024

Myocardial Infarction with Non-Obstructive Coronary Arteries (MINOCA) is a clinical entity characterized by the absence of significant coronary artery obstruction in epicardial arteries (< 50%) on coronary angiography in the setting of acute myocardial infarction (AMI). This article aims to provide a narrative review of the pathophysiological mechanisms, diagnostic challenges, and prognosis associated with MINOCA focusing only on cases associated with myocardial ischemia but of non-atherosclerotic origin. Etiological factors, including thromboembolism, coronary artery spasm, spontaneous coronary artery dissection, coronary microvascular disease, and supply-demand mismatch are addressed. Imaging modalities such as echocardiography, advances in coronary angiography like intravascular ultrasound (IVUS) and optical coherence tomography (OCT), cardiac magnetic resonance (CMR), and coronary computed tomography angiography (CCTA) are also analyzed. MINOCA patients have a better short-term prognosis compared to those with obstructive coronary artery disease but face significant long-term risks, underscoring the need for precise diagnosis and management strategies. Elevated inflammatory markers and specific genetic predispositions are also associated with adverse outcomes in MINOCA. This review focused on MINOCA from the pathophysiological perspective on ischemic, non-atherosclerotic causes, the diverse underlying mechanisms, the challenges in achieving accurate diagnosis, the importance of a tailored therapeutic approach and the necessity for further investigation of clinical outcomes.
Empirical Study of Ecological Innovation and Occupational Health Hazard among Nigerian Paddy Rice Farmers: Response to Potential Field Contamination and Climate Risks
Authors: Omoyajowo Koleayo , Ogunyebi Amos , Ogunkanmi Adebayo , Njoku Kelechi , Omoyajowo Kolawole , Oludoye Oluseye , Gupta Jyoti , Raimi Morufu
Published: August 2024

This study investigates the adoption of ecological innovations and management of occupational health hazards among Nigerian paddy rice farmers in response to field contamination and climate risks. Conducted across four major rice-producing states—Ekiti, Kwara, Edo, and Ogun—the research employed a semi-structured questionnaire developed from a comprehensive literature review and validated through peer review. Data were collected from 192 farmers through face-to-face interviews and guided dialogues, focusing on ecological innovations, vulnerability assessments, satisfaction with implemented methods, occupational health hazards, and perceptions of sustainable rice cultivation. Findings reveal that only 29.69% of farmers have adopted new measures to address contamination and climate risks, while 70.31% have not. Work-related injuries, such as cuts and abrasions, affected 28% of farmers, with minimal participation (3.1%) in safety training and support from agricultural or government agencies (1%). Correlation analysis demonstrated weak relationships among variables such as farmers' education, adoption of ecological innovations, satisfaction with results, and use of personal protective equipment (PPE). Importantly, farmers who adopted ecological innovations expressed significantly higher satisfaction (odds ratio = 11.613), as did those with prior exposure to pesticides and chemicals (odds ratio = 4.084) regarding safety practices. The study emphasizes the critical need for increased awareness, training, and support to promote sustainable farming practices and improve occupational health outcomes among paddy rice farmers in Nigeria.
Laser-Induced Breakdown Spectroscopy Detection of Heavy Metal Contamination in Soil Samples from North Birmingham, Alabama
Authors: Adhikari Nirmala , Martyshkin Dmitry , Fedorov Vladimir , Das Deblina , Antony Veena , Mirov Sergey
Published: August 2024

High levels of heavy metal contamination in soil present substantial threats to human health and the environment, leading to severe health problems such as neurotoxicity, cancer, kidney issues, chronic obstructive pulmonary disease, and reduced life expectancy. This research aims to identify and analyze heavy metals in soil samples collected from Superfund sites in North Birmingham, Alabama, specifically in affected areas with Zip codes 35207, 35217, and control area 35214. These affected areas were previously used for mining, coal-fired power plants, coke furnaces, smelting, and other potential sources of heavy metal pollution. Laser-Induced Breakdown Spectroscopy (LIBS) was employed to study 60 soil samples systematically collected from affected and control areas. We found that using LIBS, we could detect arsenic (As), lead (Pb), and manganese (Mn) in all soil samples from the affected areas. The limit of detection (LoD) was 16 ppm for Pb, 43 ppm for As, and 33 ppm for Mn using specific parameters of the detection system and/or argon gas purging at atmospheric pressure. The results were compared with ICP-MS measurements to validate the accuracy of the LIBS findings. Data showed a good linearity for all calibration data at relatively low concentrations and a good correlation between ICP-MS measurements.
Exploration of the Commonalities and Specificities in Wheat Respond to Aluminum Toxicity and Low Phosphorus via Transcriptomics and Targeted Metabolomics Study
Authors: Luo Daozhen , Li Qing , Pang Fei , Zhang Wenjie , Li Yangrui , Xing Yongxiu , Dong Dengfeng
Published: August 2024

Aluminum (Al) toxicity and low phosphorus availability (LP) are the top two co-existing edaphic constraints limiting agriculture productivity in acid soils. Plants have evolved versatile mechanisms to cope with the two stresses alone or simultaneously. However, the specific and common molecular mechanisms, especially those on flavonoids and carbohydrate metabolism, are still unclear. Laboratory studies were conducted on two wheat genotypes, Fielder (Al-tolerant and P-efficient) and Ardito (Al-sensitive and P-inefficient), exposed to 50 μM Al and 2 μM Pi (LP) in hydroponic solutions. After 4 days of stress, wheat roots were analyzed via transcriptomic and targeted metabolomic techniques. A total of 2296 differentially expressed genes (DEGs, 1535 up and 761 down) under Al and 3029 DEGs (1591 up and 1438 down) under LP were identified in Fielder. Similarly, 4404 DEGs (3191 up and 1213 down) under Al and 1430 DEGs (1176 up and 254 down) under LP were identified in Ardito. The GO annotation analysis results that 4079 DEGs annotated to the metabolic processes term. These DEGs were significantly enriched in the phenylpropanoid, flavonoid, flavone and flavonol biosynthesis, and carbohydrate metabolism pathways by performing the KEGG enrichment analysis. The targeted metabolome analysis detected 19 flavonoids and 15 carbohydrate components in Fielder and Ardito under Al and LP. More responsive genes and metabolites were involved in flavonoid metabolism under LP than Al in the Fielder, while an opposite trend in the Ardito. In the carbohydrate metabolism pathway, the genes and metabolites were higher in Fielder than in Ardito. The combined transcriptome and metabolome analysis revealed that the flavonoid and carbohydrate genes and metabolites differed between Fielder and Ardito under Al and LP, which may be an important reason for Fielder’s high Al and LP resistance. The results of this study lay a foundation for pyramiding genes and breeding multi-resistance varieties.
Assessment of Health Centers Indoor Air Quality across Various Locations in Nigeria: A Comparative Analysis of Particle Count Data
Authors: Abulude Francis Olawale , Akinnusotu Akinyinka , Oluwagbayide Samuel Dare , Arifalo Kikelomo Mabinuola , Adamu Ademola , Kenni Amoke Monisola , Sunday Acha
Published: August 2024

This study presents a comparative analysis of indoor air quality (IAQ) across health centers in Arakale, Iju, Owode, FECA, and Oba Ile, Ondo State, Nigeria. Particle count data were analyzed to identify common trends, similarities, and differences in IAQ parameters, with the aim of informing targeted interventions for improving indoor air quality in healthcare settings. The findings reveal consistent positive skewness in particle count distributions, indicating occasional spikes in pollution levels amidst generally lower concentrations. Fine particles in the size range of PC 2.5 were consistently dominant across all locations, emphasizing their significance in IAQ assessment. Similar variability in particle count data suggests comparable levels of fluctuations in IAQ among the health centers. However, variations in PCA patterns highlight unique factors influencing IAQ in specific locations, such as combustion sources and control strategies. Targeted interventions, including enhancing ventilation systems, controlling indoor pollution sources, and promoting smoking cessation programs, are proposed to mitigate health risks associated with indoor air pollution exposure. Ongoing monitoring and collaboration with environmental experts are crucial for assessing IAQ trends and implementing evidence-based interventions to safeguard the health of patients, staff, and visitors in healthcare facilities.
Transplanted Murine Tumours SPECT Imaging with 99mTc Delivered with an Artificial Recombinant Protein
Authors: Pozdniakova Natalia V. , Lipengolts Alexey A. , Skribitsky Vsevolod A. , Shpakova Kristina E. , Finogenova Yulia A. , Smirnova Anna V. , Shevelev Alexei B. , Grigorieva Elena Y.
Published: August 2024

99mTc is a well-known radionuclide widely used and readily available for medical imaging. Despite this, the 99mTc isotope is rarely utilized for tumour imaging due to the lack of agents that can selectively deliver it to tumours. However, the use of immunologically compatible recombinant proteins, which combine metal-binding sites and tumour-specific ligands, shows promise for utilizing 99mTc in the early detection of tumours. To illustrate this, a protein called E1b, containing a metal binding center with ELP repeats, tumour-specific ligands recognizing integrin αvβ3 and nucleolin, as well as a short Cys-rich sequence, was artificially designed. This protein was produced in E. coli and purified to homogeneity using metal-chelate chromatography. In experiments, C57Bl/6 and Balb/C mice with subcutaneously grafted lung carcinoma (LLC) or mammary gland adenocarcinoma (Ca755, EMT6, or 4T1) were used to test 99mTc-E1b selectivity and the kinetics of isotope retention. The mice were intravenously injected with approximately 80 MBq of 99mTc in complex with E1b, which had been freshly purified using gel-chromatography. The biodistribution of the labeled protein was then studied using the SPECT/CT imaging method within 24 h. The ratio of radionuclide distribution in the tumour versus the adjacent normal tissue (T/N) steadily increased over the 24-h period, reaching 15.7±4.2 for EMT6, 16.5±3.8 for Ca755, 6.7±4.2 for LLC, and 7.5±3.1 for 4T1.
Integrated Scale Adaptive Adjustment Factor Enhanced BlendMask Method for Pineapple Processing System
Authors: Wang Haotian , Zhang Haojian , Zhang Yukai , Deng Jieren , Liu Chengbao , Tan Jie
Published: August 2024

The study addresses the challenge of efficiently peeling pineapples, which have a distinct elliptical form, thick skin, and small eyes that are difficult to detect with conventional automated methods. This results in significant flesh waste. To improve the process, the researchers developed an integrated system combining an enhanced BlendMask method, termed SAAF-BlendMask, and a PCP method. SAAF-BlendMask improves the detection of small pineapple eyes, while PCP ensures accurate posture adjustment for precise path planning. The system uses 3D vision and deep learning technologies, achieving an average precision (AP) of 73.04% and a small object precision (APs) of 62.54% in eye detection, with a path planning success rate reaching 99%. The fully automated electromechanical system was tested on 110 real pineapples, demonstrating a reduction in flesh waste by 11.7% compared to traditional methods. This study highlights the potential of advanced machine vision and robotics in enhancing the efficiency and precision of food processing.
Extracts and Terpenoids from Stevia Species as Potential Anthelmintics for Neglected Tropical Diseases Caused by Cestode Parasites
Authors: Cevasco Contreras María del Pilar , Borgo Jimena , Celentano Ana María , Elso Orlando G. , Bach Hernán , Catalan Cesar Atilio Nazareno , Bivona Augusto Ernesto , Vaca Hugo Rolando , Rosenzvit Mara Cecilia , Sülsen Valeria Patricia
Published: August 2024

Cestodes are etiological agents of neglected diseases such as echinococcosis and cysticercosis, which are major public health problems. Antiparasitic treatment relies on a small number of approved drugs, which are often only partially effective, poorly tolerated and require prolonged administration. Thus, the discovery of novel potential treatments is critical. The Stevia genus (Asteraceae) includes species that are associated with medicinal uses and are considered a source of bioactive compounds. Here, we analyzed four South American Stevia species that have previously demonstrated antiprotozoal properties. The effect of dichloromethane extracts of these species was determined by motility assays on Mesocestoides vogae, a laboratory model of cestodes. Stevia alpina showed the highest anthelmintic potential followed by S. maimarensis and S. multiaristata, while S. aristata was the least active. The sesquiterpene lactones estafietin and eupatoriopicrin were purified from S. alpina and S. maimarensis, respectively. Estafietin showed cestocidal activity, inhibiting parasite viability in a dose-dependent manner, even from the first day of incubation. Consistent with the motility effects, the extract of S. alpina and estafietin induced major alterations on the morphology of the parasite. The results of this report show that Stevia species represent a source of new molecules with potential for the treatment of neglected tropical diseases caused by cestodes.
Threshold Spaces: The Crucial Link Study of the Transitional Spaces from the Outside (Street) to Inside of the Traditional Dwelling Units Across Various Regions of India
Authors: Saxena Ishita , Tzortzi Nerantzia Julia
Published: August 2024

Segregating one space from another is a rudimentary act of architecture and the basic principle of this act is creating differentiation between outside and inside. Thresholds, in their expanded or summed form, also produce spaces. They establish the staging for threshold areas in conjunction with space-defining elements. Threshold spaces are typically complicated spatial formations that serve as transition points. They pose a challenge to the interacting human senses in terms of perception. In this research threshold areas have been studied under a specific typology of architecture i.e the traditional housing in historic cities of India. The traditional dwelling settlements have evolved and grown over multiple layers of history, influenced by the culture, society, economy, climate and topography. The purpose of this research is to examine the characteristics of these transition spaces of the traditional dwelling units in relation to the symbolic reference and social meanings assigned to them that influence the architectural and urban spaces attached to them. It seeks to describe transition space in terms of its nature, character, morphology, and social significance. Transparency and material articulation, as well as hierarchy and enclosure, will be investigated. The importance and quality of the nature of "transition" will be examined from the inside to the outside. The research reveals that the space has great functional and socio-cultural importance. It allows multiple activities, provides protection from sun and rain, defines boundaries, connects people within the neighborhood, enhances security and gives an identity to the owner. The contributions made by this research have the potential to improve design considerations for transition areas between the dwelling units and the street.
Relationships between Six Cultural Scales and Ten Ageism Dimensions: Correlation Analysis Using Data from 31 Countries
Authors: Kokubun Keisuke
Published: August 2024

As the aging of the world accelerates, clarifying the relationship between cultural differences and ageism is an urgent issue. Therefore, in this study, we conducted a correlation analysis between the six cultural scales of Hofstede et al. [1] and the 10 ageism scales calculated from data on 35,232 people from 31 countries included in the World Values Survey Wave 6 by Inglehart et al. [2]. The results of a partial correlation analysis controlling for economic and demographic factors showed that the cultural scales were correlated with ageism. This is the first study to show that diverse cultural scales are related to multiple dimensions of ageism.
Research on strength of Bilateral Support Bearing of PDC -Cone Hybrid Bit
Authors: Liu Baxian , Yang Liyuan , Pian Xiaoxuan , Xie Rui , Chen Ting , Huang Kuilin
Published: August 2024

The existing PDC(Polycrystalline Diamond Compacts) -cone hybrid bit bearing adopts a unilateral support structure, which is prone to stress concentration in the journal area, resulting in fracture and wear failure of the bearing, thus reducing the service life of the hybrid bit. In this paper, a new type of double supported bearing hybrid bit is proposed. The static strength analysis of unilateral and bilateral support bearing structures is carried out by finite element simulation, and the stress and strain distribution of the two structures under the load of 20KN-100KN is obtained. Experimental devices for unilateral and bilateral support bearing structures are designed and manufactured to complete 50KN-100KN static pressure loading experiments. The results showed that the stress and strain of unilateral and bilateral support bearing increased linearly with the increase of load. Compared with unilateral bearing, when the load was 100KN, the maximum Mises stress of bilateral bearing decreased from 358.80MPa to 211.10MPa, with a decrease of 41.16%. The maximum contact stress decreased from 415.20MPa to 378.10MPa, decreased by 8.94%, and the maximum principal strain decreased from 11.01e-4 to 9.71e-4, decreased by 11.81%. The axial strain in the danger zone was reduced by 14.68% and 17.35%, respectively. It is found that the contact stress of the simulation data is highly correlated with the bearing life, and the service life of the bilateral bearing bit is increased by 8.94%. The simulation data and experimental results provide data support for the production of hybrid bits with bilateral bearing support.
Effects of Loading and Perforation Parameters on Rock Deformation and Damage Behaviors Induced by Dynamic Loadings Caused by the Fracturing of Tight Oil Reservoirs
Authors: Chen Bo , Aibaibu Abulimiti , Liu Yuan , Guo Xinwei , Zhou Hua , Zhao Xiangyun , Zhu Bolong
Published: August 2024

This study presents a numerical analysis of rock deformation and damage behaviors induced by dynamic loadings, specifically focusing on the fracturing of tight oil reservoirs. An elastoplastic model based on finite element methods was utilized to simulate the effects of varying loading and perforation parameters. Three distinct scenarios were modeled: a single perforation, boundary loading on three sides, and multiple perforations, each providing unique insights into the mechanical responses of the rock material. The analysis revealed that dynamic effects are highly localized around perforations and loaded boundaries across all scenarios. Acceleration magnitudes are captured with rapid attenuation observed as the distance from the perforation increased. This indicated that the highest stress concentrations and deformation occurred close to the points of loading or perforation. Strain rate analysis in the x direction showed significant fluctuations near the perforations, reflecting intense stress redistributions and compressive deformation, especially prominent in the scenario with multiple perforations. Plastic strain, representing irreversible damage, was found to concentrate near perforation tips and loaded boundaries. However, the pattern of plastic strain accumulation varied: in the small domain scenario with boundary loading, plastic strain did not immediately accumulate next to the loading boundary, whereas in scenarios with single and multiple perforations, damage developed from the loading boundaries. These findings highlight the need for precise control in perforation and dynamic loading processes to manage stress and deformation effectively. It is concluded that optimizing perforation patterns and loading strategies can enhance reservoir stimulation efficiency while minimizing unintended damage to the surrounding formation.
Anion and Cation Dynamics in Mixed-Anion Hydroborate Na3(BH4)(B12H12): 1H, 11B, and 23Na NMR Studies
Authors: Babanova Olga A. , Sadikin Yolanda , Skoryunov Roman V. , Soloninin Alexei V. , Skripov Alexander V.
Published: August 2024

Sodium borohydride-closo-hydroborate Na3(BH4)(B12H12) exhibits the high room-temperature ionic conductivity and high electrochemical stability. To study the dynamical properties of this mixed-anion compound at the microscopic level, we have measured the 1H, 11B, and 23Na nuclear magnetic resonance spectra and nuclear spin-lattice relaxation rates over the temperature range of 8 – 573 K. Our 1H and 11B spin-lattice relaxation measurements have revealed two types of reorientational jump motion. The faster motional process attributed to reorientations of the [BH4]− anions is characterized by the activation energy of 159 meV, and the corresponding reorientational jump rate reaches ~10^8 s^-1 near 130 K. The slower process ascribed to reorientations of the larger [B12H12]− anions is characterized by the activation energy of 319 meV, and the corresponding reorientational jump rate reaches ~10^8 s^-1 near 240 K. The results of the 23Na nuclear magnetic resonance measurements are consistent with the fast long-range diffusion of Na+ ions in Na3(BH4)(B12H12). The diffusive jump rate of Na+ is found to reach ~10^4 s^-1 at 300 K and ~8 × 10^8 s^-1 at 530 K. Comparison of these jump rates with the ionic conductivity data suggests the importance of correlations between diffusing ions.