bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Thermoresponsive P(NIPAAm-Co-Calix) Hydrogel: Synthesis, Characterization and its Sorption Ability for Metal Ions
Authors: Wang Meng , Yan Fan Yong , Cao Dong Lei , Song Xin Yu
Published: March 2013

A novel supramolecular smart poly(N-isopropylacrylamide-co-tetra(5-hexenyloxy)-p- tert-butylcalix[4]arene) (P(NIPAAm-co-Calix)) hydrogel was synthesized by free radical polymerization of N-isopropylacrylamine(NIPAAm) and modified calix[4]arene. The swelling behaviours and ionic adsorption capacity were studied. It is found that the polymeric network of the P(NIPAAm-co-Calix) hydrogel is characterized by an open structure with more pores and higher swelling ratio compared to the PNIPAAm hydrogel. The adsorption abilities of heavy toxic metal ions (Ni2+, Cu2+, Pb2+, Cd2+) for P(NIPAAm-co-Calix) hydrogels and PNIPAAm hydrogel were different and depend on the temperature. This could provide a theoretical basis for the intelligent response mechanism to stimulate research of supramolecular hydrogel.
Action of Flow Rate of Mobile Phase in Chromatography
Authors: Zhang Da Li , Ke Jia Jun , Lu Li Zhu
Published: March 2013

Chromatography has found an increasingly wide utilization in scientific and technological fields, even in some cases, has become indispensable methods for analysis and separation. Compared with its application, some common queries in chromatography still lack for good theoretical explanations, for instance, the action of flow rate of mobile phase on symmetry of peak. As is familiar to people, an increase in flow rate of mobile phase always causes theoretical plate number to decrease under normal conditions. At the same time, maybe the symmetry of chromatographic peak obviously increases, on the contrary. This result is self-contradictory. Why does the theoretical plate number not increase under the condition? Utilizing our prior paper on five plate numbers in chromatography, this paper points out why theoretical plate number decreases with an increase in symmetry of peak when flow rate of mobile phase increases. Based upon gas-solid chromatography results, the relation between flow rate of mobile phase and frequency of partition of solute, and the relation between flow rate and plate number are deduced. From the angle of theoretical model, this paper discusses the relation between solute partition in static mobile phase such as partition between two phases in plate theory and solute partition in flowing mobile phase such as Martin-Synge partition in slip mechanism, and their characteristics.
Experimental Research about the Application of ER Elastomer in the Shock Absorber
Authors: Zhu Shi Sha , Qian Xue Peng , He Hao , Zhang Quan Fu
Published: March 2013

When the Electrorheological elastomer (ERE) is embedded into intelligence structure system, the structure damping and stiffness of the system can be changed quickly and reversibly under an external electric field. Thus, the application of the Electrorheological elastomer in the active and passive hybrid control of structural vibration has already attracted people's wide attention. In this paper, three types of ER elastomer were prepared based on barium titanate, starch, then the microstructure of ER elastomer was observed and the mechanical properties were analyzed; a shear mode ERE shock absorber was designed, the vibration response performance of which was experimentally evaluated under various excitation frequency with or without the applied field. The experimental results showed that the damping and stiffness of the shock absorber could be modified with a changing external electric field, whose macro-features was that the damping coefficient increased with the increase of the electric field, and the damping effect in the high frequency was better than in the low frequency.
Experimental Research on Durability of Concrete Made by Seawater and Sea-Sand
Authors: Zhang Zhen Hai , Sang Zhen Qun , Zhang Le Yu , Ma Zhen Xiang , Zhang Yu
Published: March 2013

This paper introduce a series of tests about properties of seawater and sea-sand concrete, such as impermeability, dry shrinkage, alternate wetting and drying destruction and sulfate attack. The test results show that the impermeability of seawater and sea-sand concrete and ordinary concrete is basically same, the coral sand leads to decreasing of concrete impermeability, the admixture can effectively improve the impermeability of coral sand concrete. The difference between the shrinkage rate of seawater and sea-sand concrete and the ordinary concrete is not obvious, the shrinkage rate of seawater and coral-sand concrete is larger than ordinary concrete, and the admixtures can effectively inhibit the late drying shrinkage of seawater and sea-sand concrete and seawater and coral-sand concrete. In the case of alternate wetting and drying, the pressure generated by the salt crystals would not damage seawater and sea-sand concrete. Potential sulfate attack exits in seawater and sea-sand concrete, the active admixture is able to greatly enhance its ability of resisting sulfate corrosion.
Experimental Research on Mechanical Behavior of Carbon Fiber Reinforced Concrete Beam
Authors: Zhou Le , Wang Jun Wei , Liu Hong Tao
Published: March 2013

Based on the cross-section bending of 5 carbon fiber concrete beams, the mechanism of deflection and strain of carbon fiber concrete beam were studied considering the variation of the length of carbon fiber. The experimental results show that the deflection of destruction increased with the increase of the length of the carbon fiber. The carbon fiber can effectively improve the brittle failure of concrete beam, and the stain of concrete accorded with that steel bar at the same height. According to the existing test model, the theoretical calculating formula of CFRC was proposed.
Experimental Study on Parameters of Duncan-Chang Model for Cement-Soil
Authors: Qian Cai Fu , Song Xin Jiang , Wu Jie , Li Wei
Published: March 2013

The conventional triaxial tests for cement-soil show stronger soften characteristics of cement-soil under low confining pressure. The soften character is weaken as confining pressure decrease. For adopting Duncan-Chang hyperbola model to obtain parameters of cement-soil, the model cannot include the stress-strain soften characteristics of cement-soil, and must be discussed. The paper studies on parameters of Duncan-Chang model using generalized Hooke law and nonlinear elastic theory. The researches show the parameters of k and n are unrelated to soften characteristics of cement-soil; the relation of Rf and σ3 is given by the soften characteristics, Rf influence the soften process directly. The calculation method could be used in E-u model, and has good practical value.
Fabrication B-Ni-Al Shielding Materials by Vacuum Metal Infiltration
Authors: Wang Chao , Zhang Jian , Xue Xiang Xin , Cao Xiao Zhou
Published: March 2013

In this paper, B-Ni-Al shield materials were fabricated by vacuum metal infiltration. The B-Ni-Al Composite were fabricated by preheating BN+Ni at 1550°C for one hour before infiltrating Al alloys into the preforms in a vacuum at 1100°C for two hours. Microstructural characterization of the materials obtained was performed using X-ray diffraction, scanning electron microscopy(SEM). Mechanical properties were evaluated by fracture toughness and bending strength. AlN was produced with interfacial reactions between Al and BN.
Fabrication of Superhydrophobic Films on Aluminum Foils with Controllable Morphologies
Authors: Lv Yu Zhen , Wang Le Feng , Ma Kai Bo , Zhou You , Li Cheng Rong
Published: March 2013

In this study, porous architectures with controllable morphologies on Aluminum foils were fabricated by a simple solution method using zinc nitrate hexahydrate and hexamethylenetetramine as reactants. Unlike works reported previously, we show that the porous architectures on the surface of Aluminum foils can be adjusted by controlling the molar ratio and total concentration of reactants, which is proved to be of importance in tailoring the wettability of the surface. It is found that by adjusting the space of pores and the thickness of nanoslices, the water contact angle of the obtained surface can change from hydrophobic to superhydrophobic.
Influences of Different Long-Pile Length on Composite Foundation of Rigid-Long-Pile & Flexible-Short-Pile
Authors: Xu Yu Fen
Published: March 2013

To provide a theoretical basis for the optimization design of the composite foundation, we analyzed the long-pile length of rigid-long-pile & flexible-short-pile composite foundation using finite element software ANSYS as well as the effects on the whole performance of the composite foundation. The results show that under same vertical load, foundation settlement was significantly reduced with increase of long-pile length when long-pile length is less than 20m, but when the long-pile length is more than 30 m, reduction amplitude of settlement decreases significantly. When the long-pile length increases from 10m to 30m, stress distribution of the pile is similar in shape, with slowly increase of the values. It proves that long-pile length is an important parameter affecting reliability and economy of composite foundation, foundation settlement decreases with the increase of long-pile length, but if the long-pile length is longer than the effective pile length, the settlement of composite foundation will be little change.
Preparation of the Phosphating Solution Modified with Organosilicon for the Steel
Authors: Song Wei , Xu Xue Li , Ma Yu
Published: March 2013

Abstract: the anti-corrosion treatment to the steel metal with the O/W emulsion prepared by combining the organosilicon and phosphating solution not only safeguards the corrosion resisting properties of the steel surface but also promotes the bond strength between this phosphating film and the following coating. This paper discussed the preparation technologies and the construction processes of this O/W emulsion. Coating films was test by EIS and SEM, proved that the film had excellent anti-corrosion perfomences.
Research on Rust-Based Anticorrosion Coating
Authors: Lv Yao Hui , Liu Yu Xin , Zhang Shu , Xu Fu Jia
Published: March 2013

The continuous development in the field of protective coatings and the search for newer rust-based anticorrosion with improved properties have led to the emergence of rust-based anticorrosion for high performance organic coatings for corrosion protection. In this study, one such rust-based anticorrosion coating have been formulated and coated over tinplate surfaces. The physical and anticorrosive properties of the coating were evaluated by accelerated laboratory tests (chemic immersion tests). The results are reported and conclusions are drawn in this paper.
Research Progress of Flotation Process for Hematite
Authors: Wang Wei Zhi , Chen Li Peng , Yang Chun Guang
Published: March 2013

This paper briefly describes the situation of the development of China's iron and steel industry. Based on the introduction of the basis and the nature causes of hematite, the mainly used flotation process of hematite is discussed. The importance and urgency of improving technology, the research and development of new flotation reagent is put forward. The good development and utilization of hematite will have far-reaching economic and historical significance for the healthy and coordinated development of our national economy.
The Structural and Optical Properties of(Cu, Al):ZnO Films
Authors: Yang Ying Xiang , Tan Hong Lin , Ni Cheng Lin , Xiang Chao
Published: March 2013

Un-doped and (Cu, Al)-doped ZnO thin films were prepared by sol-gel spin coating technique on glass substrate. The effect of(Cu, Al)incorporation on the structural, morphological and optical properties of the Zinc oxide (ZnO)film was investigated by means of X-ray diffraction, scanning electron microscopy and UV-vis spectrophotometer. It has been found that the grain sizes, Optical band gap and the preferred orientation growth of (002) plane were decreased with increasing of (Cu, Al) dopants amount in ZnO films.
Xperimental Research on Grinding Medium and Influence Factors of SiJiaYing's Fine Grain Hematite
Authors: Liang Bing , Zhao Li Bing , Zhang Jin Rui
Published: March 2013

SiJiaYing's iron ore is fine grain hematite and gangue mineral is quartz,chlorite,black mica and parts of clay mineral.Grinding process is tend to argillation,which seriously impact the subsequent strong magnetic and flotation thick operation.This paper mainly conduct ball mill sphere diameter,ball ratio and the grinding effect factor test,which including medium filling ratio,grinding concentration and so on.By compared experiments optimize,grinding process and improve the grinding effect.
Sensitive Determination of Rutin Using a Nafion/PMB/Graphene Composite-Modified Glassy Electrode
Authors: Cai Feng , Xia Jian Fei , Wang Zong Hua , Xia Yan Zhi , Zhang Fei Fei , Xia Lin Hua
Published: March 2013

A novel Nafion/PMB/G/GCE was prepared by firstly electro-polymerization of MB to G/GCE and then coating Nafion on PMB/G/GCE. The as-prepared modified electrode combining the advantages of Nafion, methylene blue and G was employed for the sensitive detection of rutin. The results showed that the peak current of rutin obtained on Nafion/PMB/G/GCE was obviously high compared to bare electrode and G/GCE. Under the optimized value of pH, which was pH 3.0, peak current of rutin had good linear relation with the scan rate. At the same time, peak current increased linearly with increasing concentration of rutin. The linear range was from 5×10-7 mol/L to 1.2×10-5 mol/L, and the detection limit was 9.5×10-8 mol/L
Pushover Analysis of a Seismic Strengthening of an Existing Building Reinforced Concrete Structure
Authors: Attari Nasser Eddine , Chemrouk Mohamed , Amziane Sofiane
Published: March 2013

Each operation to strengthen or repair requires special consideration and requires the use of methods, tools and techniques appropriate to the situation and specific problems of each of the constructs. The aim of this paper is to study the pathology of building of reinforced concrete towards the earthquake and the vulnerability assessment using a non-linear Pushover analysis and to develop curves for a medium capacity building in order to estimate the damaged condition of the building.
Bioinformatics Analysis of UL39 Gene from Duck Plague Virus
Authors: Lu Guo Fu , Cheng An Chun , Wang Ming Shu
Published: March 2013

The structures and characteristics of the large subunit of ribonucleotide reductase (R1), encoded by UL39 gene from duck plague virus (DPV) were analyzed and predicted by using a series of free bioinformatics software packages and bioinformatics tools. The DPV UL39 gene has a base composition of 681 adenine (27.99%), 503 cytosine (20.67%), 593 guanine (24.37%) and 656 thymine (26.96%). The online analysis of the physico-chemical properties demonstrates that the protein has 40 potential phosphorylation sites and 4 N-glycosylation sites when the threshold of prediction score is above 0.5; without the signal peptide and the transmembrance region. The phylogenetic tree proved that DPV R1 protein had a close evolutionary relationship with the Mardivirus genus of the Alphaherpesviruses. In conclusion, all those results will provide some valuable information for the further research of UL39 gene.
Screening of Additives for Preparing 5% Toltrazuril Suspension Concentrate
Authors: Hao Zhi Hui , Xiao Ke Feng , Wang Lei Lei
Published: March 2013

Suitable additives are the essential parts of 5% toltrazuril suspension concentrate. This paper mainly focused on screening additives for preparing 5% toltrazuril suspension concentrate. Results show that xanthan gum is a suitable suspending agent, 1,2-propanediol is optimal wetting agent, and citric acid act excellently as flocculant.
Effect of 5-Fluorouracil on the Regulation of Zebrafish Thymidylate Synthase Gene Expression
Authors: Song Chun Xia , Zhang Qian , Xiao Yu Liang
Published: March 2013

Thymidylate synthase (TS) is an important target in cancer therapy, which is a folate-dependent enyme, catalyzing the de novo synthesis of dUMP. In this report, the effect of 5-flurorouracil (5-FU) on the regulation of TS gene expression was estimated in zebrafish. The results showed 5-FU could significantly increase the TS expression in zebrafish embryos. However, TS mRNA level were remained unchanged. To determine the effect of 5-FU and 5-FdUMP on translation of TS mRNA, a rabbit reticulocyte lysate translation system was used. Addition of 5-FU, not inhibited the translation of TS mRNA. While addition of 5-FdUMP, completely repressed the translation of TS mRNA. Therefore, induced expression of thymidylate synthase by 5-FU in zebrafish occurred in translational level, not in transcriptional level. The findings demonstrated that zebrafish TS protein was able to bind to its own cogate mRNA and the 5-FU regulated TS in the translational level. This is the first time to confirm that the regulation of TS is affected by TS and its cognant mRNA interaction in the whole animal level.
Effect of Tumstatin T-7 Peptide on HepG-2 Cells and Human Umbilical Vein Endothelial Cells
Authors: Wang Shu Jing , Wang Fei , Liu Jia , Jiang Shan , Chen Ning , Liu Lin , Zhao Ying , Zhang Xiao Dan
Published: March 2013

The direct anti-tumor T-7 peptide of tumstatin was obtained to study its effect on human hepatoma cells (HepG-2) and human umbilical vein endothelial cells (ECV304). T-7 peptide was synthesized and its purity was up to 98.5%. MTT assay, growth curve, transmission electron microscopy(TEM) were used to detect the proliferation inhibition and pro-apoptotic function. MTT experiments and growth curve experiments showed that the survival of human hepatoma cells (HepG-2) decreased in a time- and dose-dependent way with the concentration of T-7 peptide increased. T-7 peptide can induce apoptosis of HepG-2 significantly. Apoptosis features were observed by TEM. However, the inhibition of T-7 peptide on human umbilical vein endothelial cells (ECV304) was weaker. Experiments showed thatT-7 peptide can affect HepG-2 cells. It had a certain therapeutic effect on human hepatocellular carcinoma. T-7 peptide have little effect on ECV304 cells, which means it didn’t influence the formation of tumor angiogenesis and normal cells
Enzymatic Transformation from Protopanaxadiol Ginsenoside Rb1 into Rare Ginsenoside C-K and its Anti-Cancer Activity
Authors: Liao Li Min , Zhang Yang , Lin Shu Fang , Hong Su Bin , Lin Yi
Published: March 2013

Rare ginsenoside C-K was prepared using enzymatic transformation with snailase from main ginsenoside Rb1 at the following conditions: material ratio 62.14:10, pH 4.0, incubation at 46.40 °C for 15 h. After chromatography by silica gel column and crystal formation, C-K with more than 95% purity was obtained from the production of enzymatic transformation. The yield of C-K reached 63.02% after optimization, representing an about 9% increase than before. In addition, C-K we prepared exhibited an obvious inhibitory effect on the proliferation of human lung adenocarcinoma cancer cell A549 and human hepatoma cell HepG-2.
Experimental Study of Impact of Biomass Pellet Size on the Pyrolysis Products
Authors: Xue Ai Jun , Pan Ji Hong , Tian Mao Cheng
Published: March 2013

In the present study, pyrolysis characteristics of corn straw pellet were studied in biomass pyrolysis test bench. The influence of pellet diameter on pyrolysis products was investigated. The results showed that: with the increase of pellet diameter, the yield of tar decreased ,and the yield of char and gas increased, and char density increased too. The results has significant theoretical guidance on the biomass gasification and preparation of char using biomass pellets.
Expression of Aminolevulinate Dehydratase during Mycelium Development in Monascus purpureus
Authors: Xue Xiu , Gao Lan
Published: March 2013

During the initial steps of porphyrins biosynthesis, two molecules of 5-aminolevulinic acid are asymmetrically condensed to porphobilinogen by aminolevulinate dehydratase (ALAD). To investigate the relationship between the mycelium development and gene expression for ALAD in Monascus purpureus, the cDNA coding for ALAD of M.purpureus was isolated. The recombinant ALAD protein was expressed in E. coli BL21 (DE3) cells and subsequently purified using affinity chromatography. We analyzed the phylogenetic variation in metal ion usage by ALAD enzymes, and suggested that MpALAD utilize zinc to act in catalytic site. Transcriptional expression of MpALAD during M.purpureus liquid cultivation process was assayed by RT-PCR analysis on mycelium at two distinct development stages. With the transition of mycelium color from white to red, a decrease in the transcript level for MpALAD gene was observed. The results revealed that the transcriptional expression of MpALAD is developmentally regulated in M.purpureus.
Histochemical Study of early Embryo Implantation in Mice
Authors: Bai Zhi Kun , Tian Xue Chao , Cao Yun Kao , Luo Ji Long
Published: March 2013

Implantation involves a complex process between the embryo and uterus and is crucial to further embryonic development. In this histochemical study, we investigated the early stage of embryo implantation and artiﬁcial decidualization in mice by the detection of total proteins and glycosaminoglycans using toluidine blue at pH 4.0 (TB) and Xylidine Ponceau at pH 2.5 (XP). TB staining showed mild metachromatic basophilia, which was more visible in the endometrial stroma around the implantation site in day 5 of pregnancy, and the level of glycosaminoglycans was more intense near the site of implantation on day 6, 8. Histochemical staining with XP was observed more intense in the stroma close to the site of implantation, especially in day 8 of pregnancy. At sites distant from the blastocyst were observed more discrete by staining with either TB or XP. Compared with TB, XP were more evident in the stroma around the uterine lumen under artiﬁcial decidualization. This study demonstrated changes in glycosaminoglycans and proteins by the detection of anionic and cationic radicals in the endometrial stroma adjacent to the site of embryo implantation during peri-implantation, post-implantation and artiﬁcial decidualization.
Combined Oil Displacment System and Field Test of Composite Surfactant
Authors: Wu Xiao Lin , Le Jian Jun , Wang Ming Jun , Li Wei , Guo Meng Hua , Wang Rui , Bai Lu Lu , Wu Xiao Lei
Published: March 2013

Laboratory evaluation showed that rhamnolipid combined displacement system with low and wide effective concentration (0.125%~1.0%) can reduce interfacial tension with Pubei oil and water to 10-2mN/m. Injection of 0.5PV of rhamnolipid combined displacement system in natural core simulating reservoir permeability can increase recovery by 7.9%~9.3% more than water flooding. Field test for rhamnolipid combined displacement system was carried out with 2 injectors and 9 producers. Alternating injection is performed with two slugs with total volume of 19895.5m3, containing 250.9t of dosage. During 13 months of field test, stageous cumulative incremental oil production was 2014t, with 224t of average single well incremental oil. Daily oil production increased from 1.5t/d to 1.85t/d, average water cut decreased by1.5%, and input-output ratio was 1:2.4. This study provided a new way to improve oil recovery for other similar water flooded reservoirs.
Research on Glucose Oxidase Biosensor Based on Reverse Iontophoresis
Authors: Wang Hong , Liu Yang Yang , Yao Xiao Jing , Li Yan , Wu Ji Yu , Cui Jian Guo
Published: March 2013

Objective: Making a glucose sensor to detect the glucose which is extracted from the tissue fluid on reverse iontophoresis. Method: In the role of the catalysis of glucose oxidase which was fixed in polyethylene oxide gel, the glucose and potassium ferricyanide were change into gluconic acid and potassium ferrocyanide. Then we could get the concentration of glucose by detecting the current which was created by the redox reaction. Results: The glucose sensors could detect the concentration of glucose in the range of 2.2~22mmol/l and have a good linear too. The conformance test results show that the deviation of multiple measurements of the same sensor is less than 2% and the reaction time is less than 1s. Conclusion: The sensors could detect the blood glucose.
Sequence Analysis of the Cas1 Gene in Riemerella anatipestifer
Authors: He Yang , Cheng An Chun , Wang Ming Shu , Zhu De Kang , Wang Xiao Jia , Zhang Xin
Published: March 2013

Objective: The aim was to analyze Riemerella anatipestifer (RA) Cas1 gene and acquire more useful information for guiding the further study of the gene. Methods: Using bioinformatics method, sequence analysis of RA Cas1 gene was carried out with some databases and software. Results: Composition analysis of RA Cas1 gene, and homology search, sequence alignment, phylogenetic tree analysis and physicochemical property analysis of RA Cas1 protein were performed. Homology search revealed that Cas1 protein sequence of R. anatipestifer showed high similarity to those of strains within the Genus Capnocytophaga and Chryseobacterium, in accordance with the result of multiple sequence alignment and phylogenetic tree analysis. The rare codon analysis showed that there was little rare codon string in RA Cas1 gene. Conclusion: Cas1 gene of R. anatipestifer was evolutionally closely related to those of the Genus Capnocytophaga and Chryseobacterium, and can be over-expressed in E.coli without considering the impact of rare codons on translation.
Stability and Hopf Bifurcation for a HIV Infection Model with Delayed Immune Response
Authors: Zhang Xiao , Huang Dong Wei , Guo Yong Feng
Published: March 2013

In this paper, a class of HIV infection model with delayed immune response has been studied. We analyze the global asymptotic stability of the viral free equilibrium, and the stability and Hopf bifurcation of the infected equilibrium have been studied. Numerical simulations are carried out to explain the results of the analysis, and the change of the immune response of CTLs infects stability of system. These results can explain the complexity of the immune state of AIDs.
Statistic Comparison between High-Fat Gavage and Feeding to Establish Hyperlipemia Rat Model
Authors: Ma Hong Yan , Wang Fang , Du Jing Sheng
Published: March 2013

The study established hyperlipemia rat model by high-fat diets feeding and high-fat solution gavage. Body weight and related bio-physiological parameters in plasma after 30 days of treatment were tested and then statistic analysis was performed. Body weight and bio-physiological parameters had significant difference in both high-fat diets feeding and high-fat solution gavage groups when they were compared with control group. Results of statistic analysis about two groups showed that the way of high-fat solution gavaging could reduce the influence on individual difference, meanwhile it could improve the uniformity and scientificity of the data compared with the other one.
Study on Angelica sinensis Endophytic Fungi and its Antibacterial Activity
Authors: Yang Ming Jun , Wang Yong Gang , Liu Xiao Feng , Wu Jing , Qian Jiao Ling
Published: March 2013

Method Endophytic Fungi were isolated from the stem, leaves and root of Angelica sinensis by the surface sterilization method and scoring method. the antimicrobial activity from isolated endophytic fungi were detected using filter paper method with Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae as indicator microorganisms. the partial isolates were identified based on morphological characteristics and classified according to ITS-rDNA sequences analysis. Result A total of 28 endophytic were isolated from the different tissues of the Angelica sinensis, antibiotic activity of the strain AS-R109 and AS-R203 against four indicator microorganisms were the highest, with inhibiting zones diameter of 18, 22, 25, 20 and20,22,19,23 mm respectively at concentration of 10mg/mL. With morphology and molecular biology identification, AS-R109 and AS-R203 was determined to belong to the genus for Alternaria sp. and Penicillium sp., respectively.
The Role of 1H-MRS in Diagnosis of Elderly Chronic Alcoholic Toxic Encephalopathy
Authors: Ma Jun , Mu Xiao Mei , Wang Zhi Qiang , Wang Yan , Kong Fan Li
Published: March 2013

Objective: This study was undertaken to study the effect of MR imaging on patients with elderly chronic alcoholic toxic encephalopathy. Methods: 20 patients with chronic alcoholic toxic encephalopathy (CATE) and 18 patients cerebral infarction diagnosed were collected to analyze conventional imaging material and spectral spectrum line shape variation peak by magnetic resonance imaging (MRI). Results: Cerebral and cerebellar vermis atrophy and abnormal signal in the deep part of the cerebral hemispheres, basal ganglia, pons asymmetric small pieces long T1 and long T2 were detected in CATE patients. And 1H-MRS also showed that the concentrations of NAA and Cho in cerebellar vermis were significantly reduced. In cerebral infarction patients declined NAA and elevated Lac were found. Conclusion: Our study suggests that 1H-MRS play a positive role in CATE diagnosis, which keeps from further damage to the brain tissue.
Nafion/Quercetin/Graphene Composite-Modified Glassy Electrode for Selective and Sensitive Detection of Dopamine
Authors: Song Dai Min , Xia Jian Fei , Wang Zong Hua , Xia Yan Zhi , Zhang Fei Fei , Xia Lin Hua
Published: March 2013

Glassy carbon electrode (GCE) was modified using graphene (G), quercetin (Qu) and Nafion in this sequence to fabricate Nafion-Qu-G composite-modified GCE (Nafion/Qu/G/GCE). The as-prepared modified electrode combining the advantages of Nafion, Qu and G was employed for the selective and sensitive detection of dopamine (DA) in the presence of ascorbic acid (AA) and uric acid (UA). Compared with GCE, Qu/GCE, G/GCE and Qu/G/GCE, the Nafion/Qu/G/GCE was more electroactive and selective for DA. Differential pulse voltammetry (DPV) was used for electrochemical detection, the separations of the oxidation peak potentials for AA-DA and DA-UA were about 304 mV and 136 mV, which allowed selectively determining DA. In phosphate buffer solution (PBS) of pH 6.8, the Nafion/Qu/G/GCE provided a detection limit of 2.31×10-8 mol/L (S/N=3) for DA. Linearity (R=0.9963) of the peak currents against the concentration of DA was found over the range of 1.0×10-7 to 1.0×10-3 mol/L. Furthermore, the modified electrode exhibited good reproducibility and stability.
Tumstatin 7 Peptide Affect Biological Activity of B16 Melanoma Cell
Authors: Wang Shu Jing , Liu Jia , Wang Fei , Chen Ning , Jiang Shan , Liu Lin , Zhao Ying , Zhang Xiao Dan
Published: March 2013

To study the effect of biological activity of Tumstatin 7 peptide on the cell proliferation and apoptosis of B16 melanoma. Tumstatin 7 peptide was synthesized and its purity is 98.4532% by high-performance liquid chromatography. The effect of 7 peptide on B16 melanoma was observed by MTT, cell growth curve, the observation of the transmission electron microscope (TEM). 7 peptide can significantly inhibit B16 melanoma cell proliferation, showing dose- and time-dependent .Its IC50 was 72.53 μg/ml.The morphology of B16 melanoma cell was obviously changed by TEM, such as karyopyknosis and apoptotic bodies. So7 peptides can inhibit the proliferation of melanoma cells and promote melanoma cells apoptosis. It will be of great potential value to melanoma treatment.
Screening of Additives for 1% Abamectin Microcapsules Suspension
Authors: Xiao Ke Feng , Wang Zhi , Wang Lei Lei , Zhang Bao Hua , Feng Xiu Guang
Published: March 2013

Suitable additives for 1% abamectin microcapsules suspension were screened in this paper. Results show that Sodium dodecyl sulfate and NNO are suitable wetting and dispersing agent, xanthan gum act better than other four reagents as thickener, and 3% ethylene glycol is better than glycerol as antifreeze. The determination of suitable additives lays a solid foundation for the preparation of 1% abamectin microcapsules suspension.
Study on Autotoxicity of Aqueous Extracts from Different Parts of Flowering Chinese Cabbage
Authors: Liu Hou Cheng , Song Shi Wei , Chen Ri Yuan , Sun Guang Wen
Published: March 2013

Autotoxicity of aqueous extracts from roots, stems and leaves of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) were studied by hydroponic in different concentrations (0.5g/L, 2g/L, 5g/L). The results showed that in solution with aqueous extracts from roots, stems and leaves, the growth of flowering Chinese cabbage were inhibited, leaf chlorophyll content declined, photosynthesis rate were affected, activities of SOD, POD and CAT were changed, MDA concentration increased, thus cell membrane permeability of flowering Chinese cabbage was damaged. The autotoxicity of aqueous extracts in flowering Chinese cabbage increased with the extracts concentration increasing.
Enhancing Antioxidant Activity of Soluble Polysaccharide from the Submerged Fermentation Product of Cordyceps sinensis by Using Cellulase
Authors: Li Xiao Lei , Li Dan
Published: March 2013

Soluble polysaccharide is a main bioactive composition of Cordyceps sinensis. To increase its antioxidant activity, 500 mg of submerged fermentation products of C. sinensis was incubated with 7.5 U or 375 U of cellulase in 50 mL of water at 50°C for 2 h. The content of soluble polysaccharide of submerged fermentation products of C. sinensis increased from 30.28% to 33.23~38.10%. At the same concentration, the DPPH free radicals scavenging capability and reducing power of cellulase-treated soluble polysaccharide were higher than those of untreated control.
Effect of the Shape of Beans on the Properties of Delayed Luminescence and Decay Kinetic Models
Authors: Guo Jian Jun , Bai Hua , Lin Lie , Tang Guo Qing
Published: March 2013

The decay kinetic models of delayed luminescence of beans have been examined, the effect of the shape of bean samples on the properties of delayed luminescence has been investigated. It is showed the delayed luminescence of beans mainly comes from the surface of beans and can’t reflect the whole information of beans. After beans being crushed, the component of beans can be mixed together. When luminescence, the components of beans powders are all irradiated, which can reflect the whole information of beans. The fitting degree of hyperbolic function is best.
Influence of pH on Electrochemical Performances of Iron Phosphate (FePO4•xH2O) Particles and LiFePO4/C Composites
Authors: Gu Yi Jie , Liu Peng , Chen Yun Bo , Liu Hong Quan , Wang Yan Min , Hao Fei Xiang , Zhang Qing Gang , Li Shu Qi
Published: March 2013

The effect of pH concentrations on the size and morphology of FePO4•xH2O particles synthesized in a hydrothermal reactor was investigated in this work. FePO4•xH2O was prepared through co-precipitation by employing Fe(NO3)3•9H2Oand H3PO4 as raw materials. The LiFePO4 obtained through lithiation of FePO4•xH2O by using glucose as a reducing agent at 700°C. The electrochemical performance of LiFePO4 powder synthesized at 700°C were evaluated using coin cells by galvanostatic charge/discharge .The results indicated that the synthesized LiFePO4/C composites (pH=2) showed a superior electrochemical capacity of 146 mAh/g and possessed a capacity favorable cycling maintenance at the 0.1C rate and high electronic conductivity.
Mechanics Modeling upon Nano-MRM for Treatment of Intracranial Aneurysm
Authors: Sun Tiger , Jiao Shu Ping , Peng Xiang He , Xu Chang Xing , Li Jing Zhi
Published: March 2013

As a new intelligent material, Nano-Magnetorheological Material (Nano-MRM) gets a wide range of great concern to researchers and its application scope is very broad. For diagnosis and treatment cancer in one of the most difficult types of intracranial aneurysm, the work established the most practical model on Nano-MRM in intracranial aneurysm therapy research and analyzed quantitatively. The results are effective. Hope the work can be a strong impetus to human progress in the anti-cancer project.
Synthesis of a Disaccharide with a Thiol Spacer Used in Gold Nanoparticles
Authors: Wang Chao
Published: March 2013

The synthesis of a mannose-bearing disaccharide containing a thiol spacer at the reducing end was carried out to provide a tethered sugar suitable for attaching to gold nanoparticles. Such sugar is designed to mimic carbohydrates involved in cell-surface interactions. The molecule was constructed via Schmidt glycosylation of an appropriately protected glycosyl donor and an acceptor, followed by removal of protective groups and reductive amination to introduce a protected thiol spacer at the reducing end of the glycan. Subsequent removal of the thiol protective group gave the target disaccharide in a satisfactory yield.
Optimization of Formulations of 1% Abamectin Microcapsule Suspensions with Properties of Biochemical Materials
Authors: Xiao Ke Feng , Hao Zhi Hui , Wang Lei Lei , Feng Xiu Guang
Published: March 2013

By orthogonal experiments, optimal formulation of 1% abamectin microcapsule suspensions was determined as follow: the proportion of abamectin microcapsule is 1%, NNO 5.0%, sodium dodecyl sulfate 3.0%, xanthan gum 0.3%, ethylene glycol 3%, and the remainder is water. The indicators of the formulation are all excellent: good dispersibility, suspension rate greater than 90%, cold storage and hot storage syneresis rates under 5%. The properties of biochemical materials were used in optimization. The optimal formulation provides an experimental basis for industrial production of abamectin microcapsule suspensions.
Degradation of Alizarin Red Solution Using Nanometer TiO2 Film under UV Light Irradiation
Authors: Tian Peng , Sun Lu , Liu Gang , Kang Yan Hong , Duan Ji Dong , Du Wen Le
Published: March 2013

This paper explores the sol-gel titanium dioxide the conditions of experiments to determine the best conditions. The photodegradation capability of alizarin red has been enhanced by using immobilized TiO2 particles, where the titanium sol synthesized by a sol-gel method was used as the binder for the immobilization. Optimal conditions of TiO2 film, alizarin red concentration, solution pH, and UV irradiation intensity have been found to enhance the photodegradation efficiency.
The Application of Biological Materials on the Cruciate Ligament Reconstruction of Knee-Joint in Athletic Injury
Authors: Liu Kai
Published: March 2013

It is Important content that to make Surface modification and surface modification and Improve the material on the surface of the cell adhesion and promotes cell proliferation to bone tissue engineering scaffolds. The role of osteoblast and support material dependent on the Material surface characteristics, Local shape, surface energy and chemical energy, which Determine how cells adsorbed onto the surface of the material and Localization of cells and cell function behavior. Therefore, the complexity of biomaterials and cell biological material surface interaction determines the biological scaffold materials for surface modification of importance. Ideal surface modification should take into consideration the surface topology, specific identification, hydrophilic and hydrophobic protein adsorption equilibrium, and other aspects of functional new tissues. At present, the most applications in surface modification of materials is type I collagen, future research will be a variety of surface modification of materials for composite materials, which will play complementary roles, as well as gene therapy and the development of nanometer materials, it will become a hot issue in the field of bone tissue engineering.
Corrosion Behavior of Cu50Zr40Ti10 Metallic Glass in HCl and NaCl
Authors: An Wei Ke , Cai An Hui , Zhou Guo Jun , Luo Yun , Li Tie Lin , Li Xiao Song
Published: March 2013

The corrosion behavior of Cu50Zr40Ti10 (at. %) in HCl and NaCl solutions was investigated. The corrosion current densities icorr in HCl and NaCl solutions increase with increasing Clconcentration when the Cl- concentration is <0.5 molL-1, then continuously increase in the former and decrease in the latter. The icorr is larger in the latter than in the former when the Clconcentration is <0.5 molL-1, while inversely for in 1 molL-1 Cl- solution. The corrosion potential Ecorr decreases with increasing Cl- concentration in HCl. However, the change of the Ecorr vs. the chloride ion concentration in NaCl solution appears down-up-down.
Simultaneous Determination of Lomefloxacin and Ciprofloxacin in Dairy Products by First-Derivative Synchronous Spectrofluorimetry
Authors: Wang Yang , Tian Tian , Wang Lu , Hu Xiao Ya
Published: March 2013

A simple, sensitive and quick assay to simultaneously determine lomeﬂoxacin (LFLX) and ciproﬂoxacin (CPLX) had been developed by using zero-crossing first derivative constant wavelength synchronous spectrofluorimetry. Due to their similar molecule structures, it was difﬁcult to analysis and determine LFLX and CPLX simultaneously by conventional ﬂuorometry. In order to improve the sensitivity, aluminium ion was used to significantly enhance their endogenous fluorescent intensity. The different experimental parameters affecting the synchronous fluorescence intensity of the two fluoroquinolone drugs were carefully studied and optimized. The proposed method was successfully applied for the determination of the two drugs in milk samples.
The Application of Tissue Engineering Cartilage and Bracket Constructed Biomaterials for Athletic Injury
Authors: Li Bing Hui , Yu Yong , Xu Dan
Published: March 2013

The development of tissue engineering techniques for cartilage repair and regeneration provided a new way, according to their own characteristics and structure of the cartilage, as artificial cartilage replacement materials and scaffold materials should have good biomechanical properties. The effect of autologous cartilage transplantation is best in the field of articular cartilage repair, the study of bone marrow stromal cells in vitro tests and animal experiments was more, and the clinical application was less, which is still in the stage of exploration. Biomaterials material for tissue repair plays a more and more important role, especially in athletic injury.
Effect of Phosphates on Weight Gain, Thawing Loss and Cooking Yield of Frozen Pollock (Gadus morhua) Fillets
Authors: Ni Guan Pei , Zhao Qian Cheng , Li Zhi Bo , Li Jian Wei , Wang Gui Bin
Published: March 2013

Phosphate is widely used in frozen aquatic products. In this study, frozen Pollock was taken as raw materials, and the effect of different phosphates (sodium pyrophosphate, sodium tripolyphosphate and sodium hexametaphosphate) on weight gain, thawing loss and cooking yield of Pollock fillets were studied. The results showed that with the increasing concentration, the weight gain and cooking yield of sodium pyrophosphate and sodium tripolyphosphate treatment groups increased, and the thawing loss reduced, whereas sodium hexametaphosphate treatment group remained unchangeable. The optimal proportion of sodium pyrophosphate, sodium tripolyphosphate and sodium hexametaphosphate on Pollock fillets was 3:2:3 and the concentration of mixed phosphates was 4%, and the weight gain, cooking yield and thawing loss was 17.1%, 88.6% and 2.7%, respectively
The Development of Air Suspension Controller in Mechanical Engineering Based on DSP
Authors: Zhang Zhen Wei , Chen Chong , Jiao Ruo Bing , Hu Rong Rong
Published: March 2013

Cylindrical air spring suspension’s vehicle model with seven freedom degrees is established. Then the model is simulated by use of Matlab/Simulink to accomplish the simulated-computation of the driving state such as rolling angle and pitching angle. Based on the above work, air suspension controller, DSP TMS320F2812 chip as the core processor, is developed. The result of the real vehicle test proves that the controller can obviously improve vehicle’s driving smoothness and handling stability, so it meets the applying requirements.
Fault Pattern Recognition for Partial Discharge of Electrical Power Equipment Based on Properties of Electrical Materials
Authors: Chen Guo Jin , Xu Ming , Liu Ting Ting , Ni Jing , Xie Dong , Zhang Yan Ping
Published: March 2013

Partial discharge causes mainly the insulation deterioration. It is the significant symptom and manifestation, and is an important factor of the insulation failure for the electrical power equipment. On the basis of analyzing the physical model of partial discharge, this paper used the online monitoring technology of partial discharge that combines the ultra high frequency (UHF) method and the acoustic emission (AE) method, studied the fault pattern recognition method of partial discharge based on the case-based reasoning algorithm, and established the intelligent fault identification system of partial discharge based on the case-based reasoning. The system can accurately and reliably identify the fault mode type, the specific fault location and severity of partial discharge for the electrical power equipment to make the health evaluation and improve the reliability. Through the application of the new materials and new technology, the load loss of the transformer can drop by 15%, the no-load loss can decline by 50% and the fee of electricity loss can down by 32.5%.
The Design of INS under ITS Mechanical Properties for Vehicles in Mechanical Engineering
Authors: Chen Ming Qiang , Xu Kai Jun , Qian Yu
Published: March 2013

With the development of the civil aviation, the surface of airport becomes more and more crowed. The navigation systems of surface vehicles are of vital importance. The Global Positioning System (GPS) has been approved for a limited number of applications. Because it is not highly available for meeting safety-of life levels of integrity, which require timely alert if the system is unreliable for navigation under intelligent transportation system (ITS). This paper presents an analysis about the requirement and principle of an inertial navigation system (INS) and design an new mechanical architecture of INS in terms of increased availability of GPS for surface vehicles. This new mechanical architecture has a good mechanical properties in mechanical engineering.
Effect of Film Thickness and Annealing Time on Residual Stress of High-k Al2O3 Film on Si-(100) Substrate
Authors: Tang Wu , Yang Ji Jun , Li Chi Ming
Published: March 2013

In this paper, Al2O3 thin film samples were deposited on Si-(100) substrate by electron beam evaporation with different thickness at substrate temperature 400°C and after that, annealed in the air at 500°C with different time. The structure, thickness and residual stress of these films were measured by X-ray diffraction (XRD), stylus profiler and electronic thin film stress distribution tester, respectively. The effects of several parameters on the properties of Al2O3 films were studied. In addition, the relations between thickness and residual stress of Al2O3 thin films as the high-k gate dielectric was analyzed. The results shown that the residual stress becomes smaller after annealing, the residual stress was depressed down to maximum value 300MPa from 580MPa for annealing time 30min, and depressed down to minimum value 220MPa from 580MPa for annealing time 60min. But eventually, it has a critical film thickness point on the scale.
Study on the Experimental of the Electrical Discharge Machining Titanium Alloy on Gas Dielectric
Authors: Zhao Shuo Guo , Yao Xiao Min , Li Rui
Published: March 2013

In order to know the processing properties of the Electrical Discharge Machining titanium alloy on gas dielectric. Firstly, this paper does comparing experiment of EDM in gas processing titanium alloy. The results indicate that electrode dissipation is low with equifrequence square wave, positive polarity and low supply voltage to machining titanium alloy when pulse width is narrow. Secondly, this paper has done the orthogonal experiment and the variance analysis of experimental results, Its result shows that pulse width and peak current have a great influence on the machining efficiency .