bluuebunny/crossref_metadata_2025 huggingface dataset:
HIDTI: integration of heterogeneous information to predict drug-target interactions
Authors: Soh Jihee , Park Sejin , Lee Hyunju
Published: March 2022

Identification of drug-target interactions (DTIs) plays a crucial role in drug development. Traditional laboratory-based DTI discovery is generally costly and time-consuming. Therefore, computational approaches have been developed to predict interactions between drug candidates and disease-causing proteins. We designed a novel method, termed heterogeneous information integration for DTI prediction (HIDTI), based on the concept of predicting vectors for all of unknown/unavailable heterogeneous drug- and protein-related information. We applied a residual network in HIDTI to extract features of such heterogeneous information for predicting DTIs, and tested the model using drug-based ten-fold cross-validation to examine the prediction performance for unseen drugs. As a result, HIDTI outperformed existing models using heterogeneous information, and was demonstrating that our method predicted heterogeneous information on unseen data better than other models. In conclusion, our study suggests that HIDTI has the potential to advance the field of drug development by accurately predicting the targets of new drugs.
Stability, dissolution, and cytotoxicity of NaYF4-upconversion nanoparticles with different coatings
Authors: Bastos Verónica , Oskoei Párástu , Andresen Elina , Saleh Maysoon I. , Rühle Bastian , Resch-Genger Ute , Oliveira Helena
Published: March 2022

Upconversion nanoparticles (UCNPs) have attracted considerable attention owing to their unique photophysical properties. Their utilization in biomedical applications depends on the understanding of their transformations under physiological conditions and their potential toxicity. In this study, NaYF4:Yb,Er UCNPs, widely used for luminescence and photophysical studies, were modified with a set of four different coordinatively bound surface ligands, i.e., citrate, alendronate (AA), ethylendiamine tetra(methylene phosphonate) (EDTMP), and poly(maleic anhydride-alt-1-octadecene) (PMAO), as well as silica coatings with two different thicknesses. Subsequently, the aging-induced release of fluoride ions in water and cell culture media and their cytotoxic profile to human keratinocytes were assessed in parallel to the cytotoxic evaluation of the ligands, sodium fluoride and the lanthanide ions. The cytotoxicity studies of UCNPs with different surface modifications demonstrated the good biocompatibility of EDTMP-UCNPs and PMAO-UCNPs, which is in line with the low amount of fluoride ions released from these samples. An efficient prevention of UCNP dissolution and release of cytotoxic ions, as well as low cytotoxicity was also observed for UCNPs with a sufficiently thick silica shell. Overall, our results provide new insights into the understanding of the contribution of surface chemistry to the stability, dissolution behavior, and cytotoxicity of UCNPs. Altogether, the results obtained are highly important for future applications of UCNPs in the life sciences and bioimaging studies.
In-situ neutron diffraction study of lattice deformation behaviour of commercially pure titanium at cryogenic temperature
Authors: Lee Min-Su , Kawasaki Takuro , Yamashita Takayuki , Harjo Stefanus , Hyun Yong-Taek , Jeong Youngung , Jun Tea-Sung
Published: March 2022

Titanium has a significant potential for the cryogenic industrial fields such as aerospace and liquefied gas storage and transportation due to its excellent low temperature properties. To develop and advance the technologies in cryogenic industries, it is required to fully understand the underlying deformation mechanisms of Ti under the extreme cryogenic environment. Here, we report a study of the lattice behaviour in grain families of Grade 2 CP-Ti during in-situ neutron diffraction test in tension at temperatures of 15–298 K. Combined with the neutron diffraction intensity analysis, EBSD measurements revealed that the twinning activity was more active at lower temperature, and the behaviour was complicated with decreasing temperature. The deviation of linearity in the lattice strains was caused by the load-redistribution between plastically soft and hard grain families, resulting in the three-stage hardening behaviour. The lattice strain behaviour further deviated from linearity with decreasing temperature, leading to the transition of plastically soft-to-hard or hard-to-soft characteristic of particular grain families at cryogenic temperature. The improvement of ductility can be attributed to the increased twinning activity and a significant change of lattice deformation behaviour at cryogenic temperature.
Characterization of γ-H2AX foci formation under alpha particle and X-ray exposures for dose estimation
Authors: Lee Ui-Seob , Lee Dong-Hyun , Kim Eun-Hee
Published: March 2022

DNA double-strand break (DSB) induction is one of the phenotypes of cellular damage from radiation exposure and is commonly quantified by γ-H2AX assay with the number of excess fluorescent foci per cell as the main component. However, the number of foci alone may not fully characterize the state of DNA damage following exposures to different radiation qualities. This study investigated the feasibility of utilizing the focus size distribution and dephosphorylation rate of γ-H2AX to identify the type of causative radiation and dose. Human lung epithelial cells and mouse vascular endothelial cells were used to observe the expression changes of γ-H2AX foci due to alpha particle and X-ray exposures. Results showed that the average number of excess foci per cell linearly increased with the dose. The focus size distribution showed a consistent pattern depending on the causative radiation type. Three criteria for the identification of causative radiation type were derived from experimental focus size distributions and were validated in blind testing with correct identification of 27 out of 32 samples. The dose could be estimated based on the proportionality constant specific to the identified radiation type with a difference of less than 15% from the actual value. The different dephosphorylation rates of γ-H2AX produced from alpha particle and X-ray exposures were effectively utilized to determine the individual dose contributions of alpha particles and X-rays under mixed beam exposure. Individual doses were estimated to have differences of less than ~ 12% from actual values.
New Acetamidine Cu(II) Schiff base complex supported on magnetic nanoparticles pectin for the synthesis of triazoles using click chemistry
Authors: Khashei Siuki Hossein , Ghamari Kargar Pouya , Bagherzade Ghodsieh
Published: March 2022

In this project, the new catalyst copper defines as Fe3O4@Pectin@(CH2)3-Acetamide-Cu(II) was successfully manufactured and fully characterized by different techniques, including FT-IR, XRD, TEM, FESEM, EDX, VSM, TGA, and ICP analysis. All results showed that copper was successfully supported on the polymer‐coated magnetic nanoparticles. One of the most important properties of a catalyst is the ability to be prepared from simple materials such as pectin that’s a biopolymer that is widely found in nature. The catalytic activity of Fe3O4@Pectin@(CH2)3-Acetamide-Cu(II) was examined in a classical, one pot, and the three-component reaction of terminal alkynes, alkyl halides, and sodium azide in water and observed, proceeding smoothly and completed in good yields and high regioselectivity. The critical potential interests of the present method include high yields, recyclability of catalyst, easy workup, using an eco-friendly solvent, and the ability to sustain a variety of functional groups, which give economical as well as ecological rewards. The capability of the nanocomposite was compared with previous works, and the nanocomposite was found more efficient, economical, and reproducible. Also, the catalyst can be easily removed from the reaction solution using an external magnet and reused for five runs without reduction in catalyst activity.
Correlates of engaging in sports and exercise volunteering among older adults in Japan
Authors: Tsuji Taishi , Kanamori Satoru , Yamakita Mitsuya , Sato Ayane , Yokoyama Meiko , Miyaguni Yasuhiro , Kondo Katsunori
Published: March 2022

This study aimed to identify factors associated with engaging in sports and exercise volunteering among older adults. We used cross-sectional data from the Japan Gerontological Evaluation Study (JAGES), a nationwide mail survey of 20,877 older adults from 60 municipalities. Multilevel mixed-effects logistic regression analysis was used to investigate the correlation between engaging in sports and exercise volunteering and 39 variables classified into five factors: (1) demographic and biological, (2) behavioral, (3) psychological, cognitive, and emotional, (4) social and cultural, and (5) environmental factors. Among the analyzed samples, 1580 (7.6%) participants volunteered a few times/year or more often. Factors that showed positive association with the volunteering were older age, a current drinking habit, excellent self-rated health, high proportion of sports group participants in a living area, low municipal population density, and rich social and cultural features (i.e., social cohesion, support, network, and participation). Meanwhile, those that had a negative association were women, low level of education, deteriorated instrumental activities of daily living, having a past or current smoking habit, poor self-rated health, and depressive symptoms. We clarified the characteristics of the population that is more likely to participate in sports and exercise volunteering as well as those of the population that is less likely to participate and requires support.
Outburst of pest populations in rice-based cropping systems under conservation agricultural practices in the middle Indo-Gangetic Plains of South Asia
Authors: Kumar Rakesh , Choudhary Jaipal Singh , Mishra Janki Sharan , Mondal Surajit , Poonia Shishpal , Monobrullah Mohammad , Hans Hansraj , Verma Mausam , Kumar Ujjwal , Bhatt Bhagwati Prasad , Malik Ram Kanwar , Kumar Virender , McDonald Andrew
Published: March 2022

Conservation agriculture (CA), which encompasses minimum soil disturbance, residue retention either through crop residue, or cover crops and crop diversification-based crop management practices can modify the status of pest dynamics and activities under the changing climatic scenarios. CA has been advocated extensively to optimize the use of available resources, maintain the environmental quality, enhance crop productivity, and reduce the climate change impacts. Information related to the impacts of long-term CA-production systems under rice-based cropping systems on pest status is lacking, particularly in middle Indo-Gangetic Plains (MIGP). Under CA, puddling is completely avoided, and rice is directly sown or transplanted to maintain better soil health. Different sets of experimentations including farmers practice, partial CA and full CA (CA) as treatments in rice-based cropping systems, were established from 2009, 2015 and 2016 to understand the long-term impacts of CA on pest dynamics. In this study, direct and indirect effects of tillage (zero, reduced and conventional tillage), residue retention and cropping sequences on abundance and damage by pests were investigated. After 4–5 years of experimentation, populations of oriental armyworm [Mythinma (Leucania) (Pseudaletia) separata (Wlk.)] in wheat, mealybug [Brevennia rehi (Lindinger)] and bandicoot rat [Bandicota bengalensis (Gray)] in rice were found to increase abnormally in CA-based production systems. Conventionally tilled plots had a significant negative effect while residue load in zero-tilled plots had a significant positive effect on larval population build-up of M. separata. Zero tillage had a higher infestation of mealybug (52–91% infested hills) that used grassy weeds (Echinochloa colona, Echinochloa crusgalli, Cynodon dactylon, Leptochloa chinensis and Panicum repense) as alternate hosts. Cropping sequences and no disturbance of soil and grassy weeds had higher live burrow counts (4.2 and 13.7 burrows as compared to 1.47 and 7.53 burrows per 62.5 m2 during 2019–2020 and 2020–2021, respectively) and damaged tillers (3.4%) in CA-based practices. Based on the present study, pest management strategies in CA need to be revisited with respect to tillage, residue retention on soil surface, grassy weeds in field and cropping sequences to deliver the full benefits of CA in MIGP to achieve the sustainable development goals under the climate change scenarios.
MicroRNome analysis generates a blood-based signature for endometriosis
Authors: Bendifallah Sofiane , Dabi Yohann , Suisse Stéphane , Jornea Ludmila , Bouteiller Delphine , Touboul Cyril , Puchar Anne , Daraï Emile
Published: March 2022

Endometriosis, characterized by endometrial-like tissue outside the uterus, is thought to affect 2–10% of women of reproductive age: representing about 190 million women worldwide. Numerous studies have evaluated the diagnostic value of blood biomarkers but with disappointing results. Thus, the gold standard for diagnosing endometriosis remains laparoscopy. We performed a prospective trial, the ENDO-miRNA study, using both Artificial Intelligence (AI) and Machine Learning (ML), to analyze the current human miRNome to differentiate between patients with and without endometriosis, and to develop a blood-based microRNA (miRNA) diagnostic signature for endometriosis. Here, we present the first blood-based diagnostic signature obtained from a combination of two robust and disruptive technologies merging the intrinsic quality of miRNAs to condense the endometriosis phenotype (and its heterogeneity) with the modeling power of AI. The most accurate signature provides a sensitivity, specificity, and Area Under the Curve (AUC) of 96.8%, 100%, and 98.4%, respectively, and is sufficiently robust and reproducible to replace the gold standard of diagnostic surgery. Such a diagnostic approach for this debilitating disorder could impact recommendations from national and international learned societies.
Automatic compensation enhances the orientation perception in chronic astigmatism
Authors: Son Sangkyu , Shim Won Mok , Kang Hyungoo , Lee Joonyeol
Published: March 2022

Astigmatism is a prevalent optical problem in which two or more focal points blur the retinal image at a particular meridian. Although many features of astigmatic vision, including orientation perception, are impaired at the retinal image level, the visual system appears to partly restore perceptual impairment after an extended period of astigmatism. However, the mechanism of orientation perception restoration in chronic astigmatism has not yet been clarified. We investigated the notable reduction of perceptual error in chronic astigmatism by comparing the orientation perception of a chronic astigmatism group with the perception of a normal-vision group, in which astigmatism was transiently induced. We found that orientation perception in the chronic group was more accurate than in the normal vision group. Interestingly, the reduction of perceptual errors was automatic; it remained even after the optical refractive errors were fully corrected, and the orientation perception was much more stable across different orientations, despite the uneven noise levels of the retinal images across meridians. We provide here a mechanistic explanation for how the compensation of astigmatic orientation perception occurred, using neural adaptation to the biased distribution of orientations.
Challenging presumed technological superiority when working with (artificial) colleagues
Authors: Rieger Tobias , Roesler Eileen , Manzey Dietrich
Published: March 2022

Technological advancements are ubiquitously supporting or even replacing humans in all areas of life, bringing the potential for human-technology symbiosis but also novel challenges. To address these challenges, we conducted three experiments in different task contexts ranging from loan assignment over X-Ray evaluation to process industry. Specifically, we investigated the impact of support agent (artificial intelligence, decision support system, or human) and failure experience (one vs. none) on trust-related aspects of human-agent interaction. This included not only the subjective evaluation of the respective agent in terms of trust, reliability, and responsibility, when working together, but also a change in perspective to the willingness to be assessed oneself by the agent. In contrast to a presumed technological superiority, we show a general advantage with regard to trust and responsibility of human support over both technical support systems (i.e., artificial intelligence and decision support system), regardless of task context from the collaborative perspective. This effect reversed to a preference for technical systems when switching the perspective to being assessed. These findings illustrate an imperfect automation schema from the perspective of the advice-taker and demonstrate the importance of perspective when working with or being assessed by machine intelligence.
Evaluation of choroidal melanin-containing tissue in healthy Japanese subjects by polarization-sensitive optical coherence tomography
Authors: Miura Masahiro , Makita Shuichi , Yasuno Yoshiaki , Iwasaki Takuya , Azuma Shinnosuke , Mino Toshihiro , Yamaguchi Tatsuo
Published: March 2022

In this study, the choroidal melanin content in healthy eyes was evaluated with polarization-sensitive optical coherence tomography (PS-OCT). We evaluated 105 healthy eyes of 105 Japanese subjects. The mean thickness of melanin-containing tissue in the choroid (thickness of MeCh) and the choroidal melanin occupancy rate within a 5-mm circular region from the foveal center were calculated using the degree of polarization uniformity obtained by PS-OCT and compared with the choroidal thickness, patient age, and axial length. To evaluate regional variations, the 5-mm circular region was divided into a center area and an outer ring area, and the outer ring area was further divided into four areas (nasal, temporal, superior, and inferior). The mean thickness of MeCh showed a significant positive correlation with the choroidal thickness. The mean choroidal melanin occupancy rate showed a significant positive correlation with age. The mean choroidal melanin occupancy rate of the center area was significantly larger than that of the outer ring area. The mean thickness of MeCh and choroidal melanin occupancy rate of the nasal area were significantly lower than those of other areas. The distribution of melanin-containing tissue in the choroid varies significantly with age and location.
Evaluation of hepatitis C virus antibody assay using dried blood spot samples
Authors: Holzmayer Vera , Taylor Russell , Kuhns Mary C. , Gawel Susan H. , Ndembi Nicaise , Mbanya Dora , Kaptue Lazare , Rodgers Mary A. , Cloherty Gavin
Published: March 2022

Early diagnosis of hepatitis C virus (HCV) infection is essential for prompt initiation of treatment and prevention of transmission, yet several logistical barriers continue to limit access to HCV testing. Dried blood spot (DBS) technology involves a simple fingerstick that eliminates the need for trained personnel, and DBS can be stored and transported at room temperature. We evaluated the use of DBS whole blood samples in the modified Abbott ARCHITECT anti-HCV assay, comparing assay performance against the standard assay run using DBS and venous plasma samples. 144 HCV positive and 104 HCV negative matched venous plasma and whole blood specimens were selected from a retrospective study with convenience sampling in Cameroon. Results obtained using a modified volume DBS assay were highly correlated to the results of the standard assay run with plasma on clinical samples and dilution series (R2 = 0.71 and 0.99 respectively). The ARCHITECT Anti-HCV assay with input volume modification more accurately detects HCV antibodies in DBS whole blood samples with 100% sensitivity and specificity, while the standard assay had 90.97% sensitivity. The use of DBS has the potential to expand access to HCV testing to underserved or marginalized populations with limited access to direct HCV care.
An animal model of limitation of gut colonization by carbapenemase-producing Klebsiella pneumoniae using rifaximin
Authors: Xenofontos Eleni , Renieris Georgios , Kalogridi Maria , Droggiti Dionyssia-Eirini , Synodinou Kalliopi , Damoraki Georgia , Koufargyris Panagiotis , Sabracos Labros , Giamarellos-Bourboulis Evangelos J.
Published: March 2022

Current knowledge suggests that infection by carbapenem-resistant enterobacteria is preceded by gut colonization. It is hypothesized that colonization is eradicated by non-absorbable antibiotics like rifaximin. We investigated the effect of rifaximin against carbapenem-resistant Klebsiella pneumoniae (CRKP) in vitro and in a mouse model. We studied the in vitro efficacy of rifaximin against 257 CRKP clinical isolates, 188 KPC producers and 69 OXA-48 producers, by minimum inhibitory concentration and time-kill assays. We then developed a model of gut colonization by feeding 30 C57Bl6 mice with 108 cfu of one KPC-KP isolate for 7 days; mice were pre-treated orally with saline, omeprazole or ampicillin. Then, another 60 mice with established KPC-2 gut colonization received orally for 7 consecutive days rifaximin 180 mg/kg dissolved in ethanol and 4% bile or vehicle. On days 0, 3 and 7 stool samples were collected; mice were sacrificed for determination of tissue outgrowth. At a concentration of 1000 μg/ml rifaximin inhibited 84.8% of CRKP isolates. Α 3 × log10 decrease of the starting inoculum was achieved by 100, 250 and 500 μg/ml of rifaximin after 24 h against 25, 55 and 55% of isolates. Pre-treatment with ampicillin was necessary for gut colonization by KPC-KP. Treatment with rifaximin succeeded in reducing KPC-KP load in stool and in the intestine. Rifaximin inhibits at clinically meaningful gut concentrations the majority of CRKP isolates and is efficient against gut colonization by KPC-KP.
Discriminant analysis and binary logistic regression enable more accurate prediction of autism spectrum disorder than principal component analysis
Authors: Hassan Wail M. , Al-Dbass Abeer , Al-Ayadhi Laila , Bhat Ramesa Shafi , El-Ansary Afaf
Published: March 2022

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impaired social interaction and restricted, repetitive behavior. Multiple studies have suggested mitochondrial dysfunction, glutamate excitotoxicity, and impaired detoxification mechanism as accepted etiological mechanisms of ASD that can be targeted for therapeutic intervention. In the current study, blood samples were collected from 40 people with autism and 40 control participants after informed consent and full approval from the Institutional Review Board of King Saud University. Sodium (Na+), Potassium (K+), lactate dehydrogenase (LDH), glutathione-s-transferase (GST), and mitochondrial respiratory chain complex I (MRC1) were measured in plasma of both groups. Predictive models were established to discriminate individuals with ASD from controls. The predictive power of these five variables, individually and in combination, was compared using the area under a ROC curve (AUC). We compared the performance of principal component analysis (PCA), discriminant analysis (DA), and binary logistic regression (BLR) as ways to combine single variables and create the predictive models. K+ had the highest AUC (0.801) of any single variable, followed by GST, LDH, Na+, and MRC1, respectively. Combining the five variables resulted in higher AUCs than those obtained using single variables across all models. Both DA and BLR were superior to PCA and comparable to each other. In our study, the combination of Na+, K+, LDH, GST, and MRC1 showed the highest promise in discriminating individuals with autism from controls. These results provide a platform that can potentially be used to verify the efficacy of our models with a larger sample size or evaluate other biomarkers.
Synthesis of 1D Bi2O3 nanostructures from hybrid electrospun fibrous mats and their morphology, structure, optical and electrical properties
Authors: Matysiak Wiktor
Published: March 2022

The aim of this study was to produce Bi2O3 nanowires using a combination of sol–gel process and electrospinning methods and a solution based on a 13% solution of polyacrylonitrile (PAN) in N,N-dimethylformamide (DMF) containing 1.5 g of bismuth (III) nitrate pentahydrate (Bi(NO3)3·5H2O). The obtained fibrous composite mats were dried at room temperature for 24 h followed by the calcination process in air at two different temperatures of 400 °C and 600 °C. Analysis of the morphology of the fabricated Bi2O3 nanomaterials based on TEM images showed that the obtained ceramic structures could be classified as one-dimensional Bi2O3 nanostructures, with the sizes of the presented structures being 260 nm, 125 nm and 200 nm for diameter, and 5.5μm , 2 μm and 2.125 μm for length, respectively. Moreover, further analysis of the morphology of the obtained Bi2O3 nanostructures with the use of SEM showed that their diameters ranged from 150 to 500 nm when a calcination temperature of 400 °C was employed, while Bi2O3 nanowires with diameters ranging from 150 to 450 nm were obtained at 600 °C. To analyse the chemical composition and oscillatory transitions of atoms vibrating between the oscillatory levels in the molecules of the produced 1D nanostructures, and to determine the functional groups existing therein, EDX and FTIR were used. Transmission peaks in FTIR spectra recorded for wave numbers in the range of 400–4000 cm-1 were due to the presence of vibrations in Bi–O bonds, which correspond to the structure of Bi2O3. In addition, a detailed analysis of optical constants of one-dimensional Bi2O3 nanostructures fabricated using a combination of sol–gel process, electrospinning and calcination methods has been presented in this paper for the first time. Optical studies based on the recorded UV–Vis spectra showed that the obtained Bi2O3 nanowires were characterized by sharp absorption edges of radiation in the near-ultraviolet range, with sharp absorption edges falling at wavelengths of 400 nm, regardless of the applied temperature during the calcination process. The study of optical constants showed that the Bi2O3 nanostructures exhibited refractive indices of 2.62 and 2.53 at temperatures of 400 °C and 600 °C, respectively, while dielectric constants were 6.87 and 6.42, respectively. The final stage of the study was the determination of the width of energy gaps of the produced bismuth oxide nanostructures, which were found to be 3.19 and 2.97 eV, respectively. The presented results of morphology and optical properties of the obtained one-dimensional Bi2O3 semiconductor nanostructures indicate a potential possibility to apply this type of materials for the production of a new generation of dye-sensitized photovoltaic cells (DSSCs).
Lower glutamate and GABA levels in auditory cortex of tinnitus patients: a 2D-JPRESS MR spectroscopy study
Authors: Isler B. , von Burg N. , Kleinjung T. , Meyer M. , Stämpfli P. , Zölch N. , Neff P.
Published: March 2022

We performed magnetic resonance spectroscopy (MRS) on healthy individuals with tinnitus and no hearing loss (n = 16) vs. a matched control group (n = 17) to further elucidate the role of excitatory and inhibitory neurotransmitters in tinnitus. Two-dimensional J-resolved spectroscopy (2D-JPRESS) was applied to disentangle Glutamate (Glu) from Glutamine and to estimate GABA levels in two bilateral voxels in the primary auditory cortex. Results indicated a lower Glu concentration (large effect) in right auditory cortex and lower GABA concentration (medium effect) in the left auditory cortex of the tinnitus group. Within the tinnitus group, Glu levels positively correlated with tinnitus loudness measures. While the GABA difference between groups is in line with former findings and theories about a dysfunctional auditory inhibition system in tinnitus, the novel finding of reduced Glu levels came as a surprise and is discussed in the context of a putative framework of inhibitory mechanisms related to Glu throughout the auditory pathway. Longitudinal or interventional studies could shed more light on interactions and causality of Glu and GABA in tinnitus neurochemistry.
Divergence time estimation using ddRAD data and an isolation-with-migration model applied to water vole populations of Arvicola
Authors: Balmori-de la Puente Alfonso , Ventura Jacint , Miñarro Marcos , Somoano Aitor , Hey Jody , Castresana Jose
Published: March 2022

Molecular dating methods of population splits are crucial in evolutionary biology, but they present important difficulties due to the complexity of the genealogical relationships of genes and past migrations between populations. Using the double digest restriction-site associated DNA (ddRAD) technique and an isolation-with-migration (IM) model, we studied the evolutionary history of water vole populations of the genusArvicola, a group of complex evolution with fossorial and semi-aquatic ecotypes. To do this, we first estimated mutation rates of ddRAD loci using a phylogenetic approach. An IM model was then used to estimate split times and other relevant demographic parameters. A set of 300 ddRAD loci that included 85 calibrated loci resulted in good mixing and model convergence. The results showed that the two populations ofA. schermanpresent in the Iberian Peninsula split 34 thousand years ago, during the last glaciation. In addition, the much greater divergence from its sister species,A. amphibius, may help to clarify the controversial taxonomy of the genus. We conclude that this approach, based on ddRAD data and an IM model, is highly useful for analyzing the origin of populations and species.
A conical passive magnetic bearing with constant stiffness
Authors: Bjørk R. , Bahl C. R. H.
Published: March 2022

A nested conical passive magnetic bearing is presented. The bearing consists of a nested conical rotor inside a conical stator, i.e. two coaxial tilted rings of permanent magnets, both with a rectangular cross section. Varying the cone or tilt angle of the rotor and stator we determine the rotor radius that provides the highest force for three different magnetization cases. For this optimal rotor radius, we show that the bearing with the highest volume normalized force also has the highest stiffness, and furthermore often also the highest varying stiffness with axial displacement. Finally, we show that a conical bearings with a tilt angle of $$60^\circ$$

60
∘

has an almost constant stiffness and a linearly varying force with axial displacement, making it ideal as a bearing.
Quantified density of performance-degrading near-interface traps in SiC MOSFETs
Authors: Chaturvedi Mayank , Dimitrijev Sima , Haasmann Daniel , Moghadam Hamid Amini , Pande Peyush , Jadli Utkarsh
Published: March 2022

Characterization of near-interface traps (NITs) in commercial SiC metal–oxide–semiconductor field-effect transistors (MOSFETs) is essential because they adversely impact both performance and reliability by reducing the channel carrier mobility and causing threshold-voltage drift. In this work, we have applied a newly developed integrated-charge technique to measure the density of NITs that are active in the above-threshold region of commercial SiC MOSFETs. The results demonstrate that NITs trap about 10% of the channel electrons for longer than 500 ns.
PTEN/PI3K/Akt pathway alters sensitivity of T-cell acute lymphoblastic leukemia to l-asparaginase
Authors: Hlozkova Katerina , Hermanova Ivana , Safrhansova Lucie , Alquezar-Artieda Natividad , Kuzilkova Daniela , Vavrova Adela , Sperkova Kristyna , Zaliova Marketa , Stary Jan , Trka Jan , Starkova Julia
Published: March 2022

Childhood T-cell acute lymphoblastic leukemia (T-ALL) still remains a therapeutic challenge due to relapses which are resistant to further treatment. l-asparaginase (ASNase) is a key therapy component in pediatric T-ALL and lower sensitivity of leukemia cells to this drug negatively influences overall treatment efficacy and outcome. PTEN protein deletion and/or activation of the PI3K/Akt signaling pathway leading to altered cell growth and metabolism are emerging as a common feature in T-ALL. We herein investigated the relationship amongst PTEN deletion, ASNase sensitivity and glucose metabolism in T-ALL cells. First, we found significant differences in the sensitivity to ASNase amongst T-ALL cell lines. While cell lines more sensitive to ASNase were PTEN wild type (WT) and had no detectable level of phosphorylated Akt (P-Akt), cell lines less sensitive to ASNase were PTEN-null with high P-Akt levels. Pharmacological inhibition of Akt in the PTEN-null cells rendered them more sensitive to ASNase and lowered their glycolytic function which then resembled PTEN WT cells. In primary T-ALL cells, although P-Akt level was not dependent exclusively on PTEN expression, their sensitivity to ASNase could also be increased by pharmacological inhibition of Akt. In summary, we highlight a promising therapeutic option for T-ALL patients with aberrant PTEN/PI3K/Akt signaling.
Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis
Authors: Walski Tomasz , Grzeszczuk-Kuć Karolina , Gałecka Katarzyna , Trochanowska-Pauk Natalia , Bohara Raghvendra , Czerski Albert , Szułdrzyński Konstanty , Królikowski Wiesław , Detyna Jerzy , Komorowska Małgorzata
Published: March 2022

Photobiomodulation (PBM) in the red/near-infrared (R/NIR) spectral range has become widely recognized due to its anti-inflammatory and cytoprotective potential. We aimed to assess the effects of blood PBM on platelets function and hemolysis in an in vitro setting. Porcine blood samples were separated into four aliquots for this study, one of which served as a control, while the other three were subjected to three different NIR PBM dosages. The platelet count and functions and the plasma free haemoglobin and osmotic fragility of red blood cells were measured during the experiment. The control group had a considerable drop in platelet number, but the NIR exposed samples had more minimal and strictly dose-dependent alterations. These modifications were consistent with ADP and collagen-induced platelet aggregation. Furthermore, red blood cells that had received PBM were more resistant to osmotic stress and less prone to hemolysis, as seen by a slightly lower quantity of plasma free hemoglobin. Here we showed under well-controlled in vitro conditions that PBM reversibly inhibits platelet activation in a dose-dependent manner and reduces hemolysis.
Atypical processing pattern of gaze cues in dynamic situations in autism spectrum disorders
Authors: Liu Jia , Hu Jinsheng , Li Qi , Zhao Xiaoning , Liu Ying , Liu Shuqing
Published: March 2022

Psychological studies have generally shown that individuals with Autism Spectrum Disorder (ASD) have particularity in the processing of social information by using static or abstract images. Yet, a recent study showed that there was no difference in their use of social or non-social cues in dynamic interactive situations. To establish the cause of the inconsistent results, we added gaze cues in different directions to the chase detection paradigm to explore whether they would affect the performance of participants with ASD. Meanwhile, eye-tracking methodology was used to investigate whether the processing patterns of gaze cues were different between individuals with ASD and TD. In this study, unlike typical controls, participants with ASD showed no detection advantage when the direction of gaze was consistent with the direction of movement (oriented condition). The results suggested that individuals with ASD may utilize an atypical processing pattern, which makes it difficult for them to use social information contained in oriented gaze cues in dynamic interactive situations.
Relationship between body mass index and masticatory factors evaluated with a wearable device
Authors: Yoshimura Shogo , Hori Kazuhiro , Uehara Fumiko , Hori Shoko , Yamaga Yoshio , Hasegawa Yoko , Akazawa Kohei , Ono Takahiro
Published: March 2022

Numerous studies have evaluated the relationship between eating behavior and obesity, however few studies have objectively assessed eating behavior. Additionally, the association of masticatory behaviors with masticatory performance remains unclear. This study aimed to verify the relationship between masticatory performance and behavior measured by a wearable masticatory counter, and BMI. 365 healthy adults participated. Mastication behaviors, i.e. number of chews and bites, chewing rate, and chewing time, were measured using wearable masticatory counter while consuming one rice ball (100 g). Masticatory performance was evaluated using testing gummy jelly. Lifestyle habits including exercise, walking, and breakfast, were surveyed by questionnaire. The correlation coefficients between masticatory behaviors and performance and BMI were analyzed. Furthermore, multiple regression analysis was performed. The number of chews showed positive correlation with chewing rate, number of bites and chewing time, but no correlation with masticatory performance. BMI had weak but significant negative correlation with number of chews, bites, chewing time, and masticatory performance, but had no correlation with chewing rate. Multiple regression analysis revealed that BMI was associated with sex, age, number of chews, bites, masticatory performance, and walking speed. In conclusion, masticatory behavior and performance were not interrelated, but both were independently associated with BMI weakly.
Spinal fracture reveals an accident episode in Eremotherium laurillardi shedding light on the formation of a fossil assemblage
Authors: de S. Barbosa Fernando H. , de Araújo-Júnior Hermínio I. , da Costa Isadora , de Araújo André Vieira , Oliveira Edison Vicente
Published: March 2022

The Toca das Onças cave is one of the most important Quaternary mammal deposits of Brazil. Two different hypotheses have been proposed to explain the preservation mode of its skeletal remains: either the animals climbed down into the cave, or it could have functioned as a natural trap. Evaluation of pathological modifications on three articulated vertebrae of a single adult giant ground sloth Eremotherium laurillardi reveals a particular type of bone fracture caused by compressive force on the vertebral column, which split the vertebral bodies in the sagittal plane. This diagnosis suggests that the animal accidentally fell into the cave, in accordance with the second hypothesis proposed to the incorporation mode of skeletal remains into the cave.
Chromitite layers indicate the existence of large, long-lived, and entirely molten magma chambers
Authors: Latypov Rais , Chistyakova Sofya , Barnes Stephen J. , Godel Belinda , Delaney Gary W. , Cleary Paul W. , Radermacher Viktor J. , Campbell Ian , Jakata Kudakwashe
Published: March 2022

The classical paradigm of the ‘big magma tank’ chambers in which the melt differentiates, is replenished, and occasionally feeds the overlying volcanos has recently been challenged on various grounds. An alternative school of thought is that such large, long-lived and largely molten magma chambers are transient to non-existent in Earth’s history. Our study of stratiform chromitites in the Bushveld Complex—the largest magmatic body in the Earth’s continental crust—tells, however, a different story. Several chromitites in this complex occur as layers up to 2 m in thickness and more than 400 kms in lateral extent, implying that chromitite-forming events were chamber-wide phenomena. Field relations and microtextural data, specifically the relationship of 3D coordination number, porosity and grain size, indicate that the chromitites grew as a 3D framework of touching chromite grains directly at the chamber floor from a basaltic melt saturated in chromite only. Mass-balance estimates imply that a few km thick column of this melt is required to form each of these chromitite layers. Therefore, an enormous volume of melt appears to have been involved in the generation of all the Bushveld chromitite layers, with half of this melt being expelled from the magma chamber. We suggest that the existence of thick and laterally extensive chromitite layers in the Bushveld and other layered intrusions supports the classical paradigm of big, albeit rare, ‘magma tank’ chambers.
Joint integrity evaluation of laser beam welded additive manufactured Ti6Al4V sheets
Authors: Omoniyi P. O. , Mahamood R. M. , Arthur N. , Pityana S. , Skhosane S. , Okamoto Y. , Shinonaga T. , Maina M. R. , Jen T. C. , Akinlabi E. T.
Published: March 2022

The feasibility of joining laser metal deposited Ti6Al4V sheets using laser beam welding was investigated in this article. The additive manufactured sheets were joined using a 3 kW CW YLS-2000-TR ytterbium laser system. The mechanical properties and microstructure of the welded additive manufactured parts (AM welds) were compared with those of the wrought sheets welded using the same laser process. The welds were characterized and compared in terms of bead geometry, microhardness, tensile strength, fractography, and microstructure. The differences in characteristics are majorly found in the width of the bead and tensile strength. The bead width of AM welds appear wider than the wrought welds, and the wrought welds exhibited higher tensile strength and ductility than the AM welds.
Prevalence, awareness, and treatment of depression among community-dwelling stroke survivors in Korea
Authors: Lee Eung-Joon , Kwon Oh Deog , Kim Seung Jae
Published: March 2022

Post-stroke depression (PSD), a prevalent complication of stroke, causes poor outcomes. However, little is known about its prevalence and management among community-dwelling stroke survivors. Thus, we investigated the prevalence, awareness, and treatment of PSD in a community setting. A cross-sectional study was performed using representative data from the Korea National Health and Nutrition Surveys 2014, 2016, and 2018. A total of 11,122 participants aged ≥ 40 years were categorized, including 343 stroke survivors and 10,779 non-stroke survivors. We then calculated and compared the prevalence, awareness (formal diagnosis of depression by a doctor), and treatment rates of depression between the two groups. Depression was defined as a score ≥ 10 in the nine-item Patient Health Questionnaire (PHQ-9). Depression was significantly more prevalent among stroke survivors than in non-stroke survivors (22.2% vs. 8.5%, respectively), while the differences in the awareness and treatment rates were insignificant. However, only 46.8% of stroke survivors with PSD were aware of their condition, and only 20.5% were receiving treatment. These results suggest that clinicians should more actively screen for and treat depression among stroke survivors.
Post-buckling behaviors of thin-film soft-substrate bilayers with finite-thickness substrate
Authors: Li Meng , Sun Bohua
Published: March 2022

Surface buckling behaviors of thin-film soft-substrate bilayers have important research value. Recent research has focused on bilayers with infinite-thickness substrates. However, bilayers with finite-thickness substrates widely exist. To study this problem more comprehensively, we extended the stability theory of a beam on an elastic foundation to bilayers and then established a finite element method of bilayers using the neo-Hookean hyperelastic constitutive model. A self-contact analysis method was coupled to the finite element method so that the further buckling evolution of the film surface after folding could be simulated. Based on our analysis of various modulus ratios and thickness ratios, the evolution of the buckling path was significantly influenced by the thickness ratio. Without considering the situation of a prestressed substrate, four new buckling paths were found. Thus, we extended the single buckling path (under infinite thickness substrate) to five types. Our study also found that for path four, the substrate with a certain thickness exhibited a special final stable surface morphology. That is, regardless of the friction, a new periodic morphology after film folding appeared due to the contact slip of the film surface. Finally, further analysis showed that these five buckling paths were all dependent on different modulus ratios and thickness ratios.
Neural-based modeling adsorption capacity of metal organic framework materials with application in wastewater treatment
Authors: Parsaei Mozhgan , Roudbari Elham , Piri Farhad , El-Shafay A. S. , Su Chia-Hung , Nguyen Hoang Chinh , Alashwal May , Ghazali Sami , Algarni Mohammed
Published: March 2022

We developed a computational-based model for simulating adsorption capacity of a novel layered double hydroxide (LDH) and metal organic framework (MOF) nanocomposite in separation of ions including Pb(II) and Cd(II) from aqueous solutions. The simulated adsorbent was a composite of UiO-66-(Zr)-(COOH)2 MOF grown onto the surface of functionalized Ni50-Co50-LDH sheets. This novel adsorbent showed high surface area for adsorption capacity, and was chosen to develop the model for study of ions removal using this adsorbent. A number of measured data was collected and used in the simulations via the artificial intelligence technique. Artificial neural network (ANN) technique was used for simulation of the data in which ion type and initial concentration of the ions in the feed was selected as the input variables to the neural network. The neural network was trained using the input data for simulation of the adsorption capacity. Two hidden layers with activation functions in form of linear and non-linear were designed for the construction of artificial neural network. The model’s training and validation revealed high accuracy with statistical parameters of R2 equal to 0.99 for the fitting data. The trained ANN modeling showed that increasing the initial content of Pb(II) and Cd(II) ions led to a significant increment in the adsorption capacity (Qe) and Cd(II) had higher adsorption due to its strong interaction with the adsorbent surface. The neural model indicated superior predictive capability in simulation of the obtained data for removal of Pb(II) and Cd(II) from an aqueous solution.
Induction of resistance to Myzus persicae-nicotianae in Cucumber mosaic virus infected tobacco plants using silencing of CMV-2b gene
Authors: Karimi Kazhal , Sadeghi Amin , Maroufpoor Mostafa , Azizi Abdolbaset
Published: March 2022

Aphids such as tobacco aphidMyzus persicae-nicotianae,are among the most important plant viral vectors and plant viruses encode genes to interact with their vectors.Cucumber mosaic virus(CMV) encodes 2b protein as a suppressor of plant immune and it plays a vital role in CMV accumulation and susceptibility to aphid vectors. In this study, the resistance of tobacco plants (Nicotiana tabacum) toM. p. nicotianaewas evaluated by silencing of2bin CMV-infected plants. However, thepFGC-C.hsilencing gene construct was transiently expressed usingAgrobacterium tumefacience,LBA 4404 in tobacco leaves, and four days later, the plants were mechanically inoculated by CMV (Kurdistan isolate), and then, 15 days post-inoculation 1 nonviruliferous aphid was placed on each leaf for evaluation of resistance toM. p. nicotianae. To evaluate the tobacco plants resistance and susceptibility toM. p. nicotianae, the number of aphids existent per tobacco leaf, life table and, demographic parameters were recorded and used as a comparison indicator. The obtained results were analyzed using the age-stage, two-sex life table. The highest number of aphids was recorded on the control CMV-infected plants, while the lowest number on CMV infected leaves expressing CMV-2b silencing construct (pFGC-C.h). The obtained data revealed the lowest rate for all of intrinsic rate of natural increase (rm) (0.246/day), the rate of reproduction (r0) (17.04 females/generation), and finite rate of increase (λ) (1.279/day), on thepFGC-C.htreatment. The maximum generation time (T) (11.834 days) was observed on (V) treatment. However, the collected data revealed induction of resistance to tobacco aphids by silencing of CMV-2b in CMV infected plants.