bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved Child Mental Health Following Brief Relationship Enhancement and Co-Parenting Interventions During the Transition to Parenthood
Authors: Tomfohr-Madsen Lianne M. , Giesbrecht Gerald F. , Madsen Joshua W. , MacKinnon Anna , Le Yunying , Doss Brian
Published: January 2020

The transition to parenthood has been identified as a significant relationship stressor. Many couples report declines in relationship satisfaction and difficulty with individual stress and co-parenting—problems that have been associated with both child temperament as well as emotional and behavioral problems. Several parenting and relationship interventions have been developed to buffer against these difficulties. In the current study, we report secondary analyses of a randomized controlled trial of brief (6-h) interventions that focused on improving either relationship satisfaction or co-parenting, delivered during pregnancy and the early postpartum period. In this trial, 90 opposite-sex couples (180 participants), who were pregnant with their first child, and were assessed as being at high risk for declines in relationship satisfaction, were randomized to receive either (1) a relationship intervention, (2) a co-parenting intervention, or (3) an information control. At 12 months postpartum, couples who received either the relationship or co-parenting intervention rated their infants as having lower negative emotionality and as having fewer externalizing symptoms compared to the information-only control. Lower externalizing symptoms at 12 months were, in turn, associated with reduced externalizing symptoms at 24 months postpartum. Whereas, lower ratings of child negative emotionality at 12 months were associated with reduced internalizing symptoms at 24 months postpartum. These results indicate that brief relationship or co-parenting interventions delivered during the transition to parenthood have secondary benefits for child mental health.
Ultra-Short-Term and Short-Term Heart Rate Variability Recording during Training Camps and an International Tournament in U-20 National Futsal Players
Authors: Chen Yung-Sheng , Clemente Filipe Manuel , Bezerra Pedro , Lu Yu-Xian
Published: January 2020

The aim of this study was to examine ultra-short-term and short-term heart rate variability (HRV) in under-20 (U-20) national futsal players during pre-tournament training camps and an official tournament. Fourteen male U-20 national futsal players (age = 18.07 ± 0.73 yrs; height = 169.57 ± 8.40 cm; body weight = 64.51 ± 12.19 kg; body fat = 12.42% ± 3.18%) were recruited to participate in this study. Early morning 10 min resting HRV, Borg CR-10 scale session rating of perceived exertion (sRPE), and general wellness questionnaire were used to evaluate autonomic function, training load, and recovery status, respectively. Log-transformed root mean square of successive normal-to-normal interval differences (LnRMSSD) was used to compare the first 30 s, first 1 min, first 2 min, first 3 min, and first 4 min with standard 5 min LnRMSSD. Mean (LnRMSSDmean) and coefficient of variation (LnRMSSDcv) of LnRMSSD were used to compare the different time segments of HRV analysis. The result of LnRMSSDmean showed nearly perfect reliability and relatively small bias in all comparisons. In contrast, LnRMSSDcv showed nearly perfect reliability and relatively small bias from 2-4 min time segments in all study periods. In conclusion, for accuracy of HRV measures, 30 s or 1 min ultra-short-term record of LnRMSSDmean and short-term record of LnRMSSDcv of at least 2 min during the training camps are recommended in U-20 national futsal players.
Modeling of the Concentrations of Ultrafine Particles in the Plumes of Ships in the Vicinity of Major Harbors
Authors: Karl Matthias , Pirjola Liisa , Karppinen Ari , Jalkanen Jukka-Pekka , Ramacher Martin Otto Paul , Kukkonen Jaakko
Published: January 2020

Marine traffic in harbors can be responsible for significant atmospheric concentrations of ultrafine particles (UFPs), which have widely recognized negative effects on human health. It is therefore essential to model and measure the time evolution of the number size distributions and chemical composition of UFPs in ship exhaust to assess the resulting exposure in the vicinity of shipping routes. In this study, a sequential modelling chain was developed and applied, in combination with the data measured and collected in major harbor areas in the cities of Helsinki and Turku in Finland, during winter and summer in 2010–2011. The models described ship emissions, atmospheric dispersion, and aerosol dynamics, complemented with a time–microenvironment–activity model to estimate the short-term UFP exposure. We estimated the dilution ratio during the initial fast expansion of the exhaust plume to be approximately equal to eight. This dispersion regime resulted in a fully formed nucleation mode (denoted as Nuc2). Different selected modelling assumptions about the chemical composition of Nuc2 did not have an effect on the formation of nucleation mode particles. Aerosol model simulations of the dispersing ship plume also revealed a partially formed nucleation mode (Nuc1; peaking at 1.5 nm), consisting of freshly nucleated sulfate particles and condensed organics that were produced within the first few seconds. However, subsequent growth of the new particles was limited, due to efficient scavenging by the larger particles originating from the ship exhaust. The transport of UFPs downwind of the ship track increased the hourly mean UFP concentrations in the neighboring residential areas by a factor of two or more up to a distance of 3600 m, compared with the corresponding UFP concentrations in the urban background. The substantially increased UFP concentrations due to ship traffic significantly affected the daily mean exposures in residential areas located in the vicinity of the harbors.
Social Support and Subclinical Coronary Artery Disease in Middle-Aged Men and Women: Findings from the Pilot of Swedish CArdioPulmonary bioImage Study
Authors: Djekic Demir , Fagman Erika , Angerås Oskar , Lappas George , Torén Kjell , Bergström Göran , Rosengren Annika
Published: January 2020

Social support has been associated with coronary artery disease (CAD), particularly in individuals who have sustained a cardiovascular event. This study investigated the relationship between social support and subclinical CAD among 1067 healthy middle-aged men and women. Social support was assessed with validated social integration and emotional attachment measures. Subclinical CAD was assessed as a coronary artery calcium score (CACS) using computed tomography. There was no association between social support and CACS in men. In women, low social support was strongly linked to cardiovascular risk factors, high levels of inflammatory markers, and CACS > 0. In a logistic regression model, after adjustment for 12 cardiovascular risk factors, the odds ratio (95% confidence intervals) for CACS > 0 in women with the lowest social integration, emotional attachment, and social support groups (reference: highest corresponding group) were 2.47 (1.23–5.12), 1.87 (0.93–3.59), and 4.28 (1.52–12.28), respectively. Using a machine learning approach (random forest), social integration was the fourth (out of 12) most important risk factor for CACS > 0 in women. Women with lower compared to higher or moderate social integration levels were about 14 years older in “vascular age”. This study showed an association between lack of social support and subclinical CAD in middle-aged women, but not in men. Lack of social support may affect the atherosclerotic process and identify individuals vulnerable to CAD events.
Association between Dental Amalgam Filling and Essential Tremor: A Nationwide Population-Based Case Control Study in Taiwan
Authors: Tseng Chien-Fang , Chen Kun-Huang , Yu Hui-Chieh , Chang Yu-Chao
Published: January 2020

Essential tremor (ET) is a common neurological disorder and the most common movement disorder. Low-level occupational exposure to mercury vapor is known to be a crucial factor that increases the risk of tremor. Dental amalgam is one of the main sources of mercury in those who possess amalgam restorations. However, the relationship between ET and amalgam filling (AMF) is not quite clear. The purpose of this study was to investigate the association between AMF and the risk of ET using a population-based administrative databank. The data for this study were sourced from the Taiwanese National Health Insurance Research Database (NHIRD). A retrospective case-control study was conducted using this databank from 2000 to 2013. Case and control groups were matched by sex, age, urbanization level, monthly income, and Charlson comorbidity index using the propensity score method with a 1:1 ratio. In this study, 3008 cases and 3008 controls were included. The results from this nationwide population-based case-control study did not indicate any association between ET and AMF in Taiwan. Although the results were not significantly statistical, the findings may be worthy to be valued.
Deficit Alternate Drip Irrigation Increased Root-Soil-Plant Interaction, Tomato Yield, and Quality
Authors: Wang Jingwei , Li Yuan , Niu Wenquan
Published: January 2020

To determine the soil mechanism in root-zone caused by water saving and the production response to alternate drip irrigation (ADI), the present study investigated the effects of deficit ADI on tomato growth using the conventional surface drip irrigation (CDI) as a control. The interactions among the experimental treatments on root index, photosynthetic efficiency, biomass accumulation, yield, fruit quality and irrigation water use efficiency (IWUE) were assessed and the inner mechanism of root-soil effecting on tomato growth, photosynthate distribution, yield and quality was discussed. ADI significantly enhanced root-soil interaction, promoted soil nitrogen and phosphorus absorption by tomato and tomato growth. However, different soil moisture deficits significantly affected tomato photosynthate accumulation and distribution, as well as fruit quality. With irrigation amount of 50% field capacity (F), ADI significantly increased soluble sugar, total soluble solid and lycopene by 38.08%, 19.48% and 30.05%, respectively, compared to those of CDI, but decreased irrigation amounts by 29.86% in comparison with the CDI one. ADI of 70% F could significantly distribute more photosynthate to fruits, thus enhanced tomato yields by 24.6% and improved IWUE by 17.05% compared to that of CDI. In addition, ADI of 70% F improved tomato fruits quality, and in particular organic acid was decreased by 43.75% and sugar-acid ratio was increased by 97% compared to CDI. However, ADI of 60% F distributed more photosynthate to plant, showing no significant difference of yields in comparison with CDI and ADI of 70% F, but a higher IWUE by 19.54% than that of CDI. ADI of 60% F significantly enhanced soluble sugar, total soluble solid, soluble protein, lycopene and sugar-acid ratio in tomato fruits by 2.06, 1.26, 1.61, 1.4 and 3.2 times respectively compared to CDI. Therefore, ADI of 60% or 70% F can be overall recommended for tomato production in a greenhouse, plant growth, fruit yield and quality, and IWUE.
Diagnosis-Specific Sickness Absence and Subsequent Common Mental Disorders: A Register-Linkage Cohort Study among Finnish Public Sector Employees
Authors: Mauramo Elina , Lallukka Tea , Mänty Minna , Sumanen Hilla , Pietiläinen Olli , Lahelma Eero , Rahkonen Ossi
Published: January 2020

Sickness absence is associated with poor health outcomes, but little is known about its consequences for general mental health. This study examined the associations between diagnosis-specific sickness absence and subsequent common mental disorders (CMD). Register data on medically certified all-cause sickness absence and sickness absence due to mental disorders and musculoskeletal diseases from 2004–2007 were linked to the Helsinki Health Study 2007 and 2012 survey data on City of Helsinki employees in Finland (N = 3560). Using logistic regression and multinomial logistic regression, we analysed the associations between the total number of reimbursed sickness absence days in 2004-7 and CMD General Health Questionnaire 12) in 2007 and 2012 and CMD changes. Sickness absence due to mental disorders (age- and sex-adjusted odds ratio (OR)range: 2.16 to 2.93), musculoskeletal diseases (OR range: 2.79 to 2.93) and all-cause sickness absence (OR range: 1.48 to 3.20) were associated with CMD in 2007. In 2012, associations with lower ORs were observed. Associations were also found with changing and especially repeated (OR range: 1.49 to 3.40) CMD. The associations remained after adjusting for work-related covariates and health behaviours. Diagnosis-specific sickness absence showed persistent associations with subsequent CMD and their changes. Attention should be paid to both the short- and long-term consequences of sickness absence for employee mental health.
Effect of Alkalinity on Catalytic Activity of Iron–Manganese Co-Oxide in Removing Ammonium and Manganese: Performance and Mechanism
Authors: Cheng Ya , Zhang Shasha , Huang Tinglin , Hu Feifan , Gao Minyi , Niu Xiruo
Published: January 2020

In this study, a pilot-scale experimental filter system was used to investigate the effect of bicarbonate alkalinity on the activity of an Fe–Mn co-oxide for ammonium and manganese removal from surface water. The results showed that an increase in alkalinity to 150 mg/L (calculated as CaCO3) by the addition of NaHCO3 significantly promoted the activity of the Fe–Mn co-oxide. The ammonium and manganese removal efficiencies of the Fe–Mn co-oxide increased from 40% to 95% and 85% to 100%, respectively. After NaHCO3 was no longer added, the activity of the filter column remained. Moreover, pH (7.4–8.0) and temperature (12.0–16.0 °C) were not the main factors affecting the activity of the filter, and had no significant effect on the activity of the filter. Further characterization analysis of the Fe–Mn co-oxide filter film showed that after alkalinity was increased, the accumulation of aluminum on the filter media surface decreased from 3.55% to 0.16% and the oxide functional groups changed. This was due to the action of bicarbonate and the residual aluminum salt coagulant in the filter, which caused the loss of Al from the surface of the filter media and weakened the influence of the aluminum salt coagulant on the activity of the Fe–Mn co-oxide; hence, the activity was recovered.
Methodological Quality of Manuscripts Reporting on the Usability of Mobile Applications for Pain Assessment and Management: A Systematic Review
Authors: Almeida Ana F. , Rocha Nelson P. , Silva Anabela G.
Published: January 2020

Background: There has been increasing use of mobile mHealth applications, including pain assessment and pain self-management apps. The usability of mHealth applications has vital importance as it affects the quality of apps. Thus, usability assessment with methodological rigor is essential to minimize errors and undesirable consequences, as well as to increase user acceptance. Objective: this study aimed to synthesize and evaluate existing studies on the assessment of the usability of pain-related apps using a newly developed scale. Methods: an electronic search was conducted in several databases, combining relevant keywords. Then titles and abstracts were screened against inclusion and exclusion criteria. The eligible studies were retrieved and independently screened for inclusion by two authors. Disagreements were resolved by discussion until consensus was reached. Results: a total of 31 articles were eligible for inclusion. Quality assessment revealed that most manuscripts did not assess usability using valid instruments or triangulation of methods of usability assessment. Most manuscripts also failed to assess the three domains of usability (effectiveness, efficiency and satisfaction). Conclusions: future studies should consider existing guidelines on usability assessment design, development and assessment of pain-related apps.
RNA Editing as a Therapeutic Approach for Retinal Gene Therapy Requiring Long Coding Sequences
Authors: Fry Lewis E. , Peddle Caroline F. , Barnard Alun R. , McClements Michelle E. , MacLaren Robert E.
Published: January 2020

RNA editing aims to treat genetic disease through altering gene expression at the transcript level. Pairing site-directed RNA-targeting mechanisms with engineered deaminase enzymes allows for the programmable correction of G>A and T>C mutations in RNA. This offers a promising therapeutic approach for a range of genetic diseases. For inherited retinal degenerations caused by point mutations in large genes not amenable to single-adeno-associated viral (AAV) gene therapy such as USH2A and ABCA4, correcting RNA offers an alternative to gene replacement. Genome editing of RNA rather than DNA may offer an improved safety profile, due to the transient and potentially reversible nature of edits made to RNA. This review considers the current site-directing RNA editing systems, and the potential to translate these to the clinic for the treatment of inherited retinal degeneration.
Osteogenic Potential of Bovine Bone Graft in Combination with Laser Photobiomodulation: An Ex Vivo Demonstrative Study in Wistar Rats by Cross-Linked Studies Based on Synchrotron Microtomography and Histology
Authors: Luca Ruxandra Elena , Giuliani Alessandra , Mănescu Adrian , Heredea Rodica , Hoinoiu Bogdan , Constantin George Dumitru , Duma Virgil-Florin , Todea Carmen Darinca
Published: January 2020

Background: Alveolar bone defects are usually the main concern when planning implant treatments for the appropriate oral rehabilitation of patients. To improve local conditions and achieve implant treatments, there are several methods used for increasing bone volume, among which one of the most successful, versatile, and effective is considered to be guided bone regeneration. The aim of this demonstrative study was to propose an innovative analysis protocol for the evaluation of the effect of photobiomodulation on the bone regeneration process, using rat calvarial defects of 5 mm in diameter, filled with xenograft, covered with collagen membrane, and then exposed to laser radiation. Methods: The animals were sacrificed at different points in time (i.e., after 14, 21, and 30 days). Samples of identical dimensions were harvested in order to compare the results obtained after different periods of healing. The analysis was performed by cross-linking the information obtained using histology and high-resolution synchrotron-based tomography on the same samples. A comparison was made with both the negative control (NC) group (with a bone defect which was left for spontaneous healing), and the positive control (PC) group (in which the bone defects were filled with xenografts and collagen membrane without receiving laser treatment). Results: We demonstrated that using photobiomodulation provides a better healing effect than when receiving only the support of the biomaterial. This effect has been evident for short times treatments, i.e., during the first 14 days after surgery. Conclusion: The proposed analysis protocol was effective in detecting the presence of higher quantities of bone volumes under remodeling after photobiomodulation with respect to the exclusive bone regeneration guided by the xenograft.
Genome Sequence and QTL Analyses Using Backcross Recombinant Inbred Lines (BILs) and BILF1 Lines Uncover Multiple Heterosis-related Loci
Authors: Yu Yahui , Zhu Mengmeng , Cui Yue , Liu Yu , Li Zhenyu , Jiang Nan , Xu Zhengjin , Xu Quan , Sui Guomin
Published: January 2020

Heterosis is an interesting topic for both breeders and biologists due to its practical importance and scientific significance. Cultivated rice (Oryza sativa L.) consists of two subspecies, indica and japonica, and hybrid rice is the predominant form of indica rice in China. However, the molecular mechanism underlying heterosis in japonica remains unclear. The present study determined the genome sequence and conducted quantitative trait locus (QTL) analysis using backcross recombinant inbred lines (BILs) and BILF1 lines to uncover the heterosis-related loci for rice yield increase under a japonica genetic background. The BIL population was derived from an admixture variety Habataki and japonica variety Sasanishiki cross to improve the genetic diversity but maintain the genetic background close to japonica. The results showed that heterosis in F1 mainly involved grain number per panicle. The BILF1s showed an increase in grain number per panicle but a decrease in plant height compared with the BILs. Genetic analysis then identified eight QTLs for heterosis in the BILF1s; four QTLs were detected exclusively in the BILF1 population only, presenting a mode of dominance or super-dominance in the heterozygotes. An additional four loci overlapped with QTLs detected in the BIL population, and we found that Grains Height Date 7 (Ghd7) was correlated in days to heading in both BILs and BILF1s. The admixture genetic background of Habataki was also determined by subspecies-specific single nucleotide polymorphisms (SNPs). This investigation highlights the importance of high-throughput sequencing to elucidate the molecular mechanism of heterosis and provides useful germplasms for the application of heterosis in japonica rice production.
The “Janus Face” of Platelets in Cancer
Authors: Catani Maria Valeria , Savini Isabella , Tullio Valentina , Gasperi Valeria
Published: January 2020

Besides their vital role in hemostasis and thrombosis, platelets are also recognized to be involved in cancer, where they play an unexpected central role: They actively influence cancer cell behavior, but, on the other hand, platelet physiology and phenotype are impacted by tumor cells. The existence of this platelet-cancer loop is supported by a large number of experimental and human studies reporting an association between alterations in platelet number and functions and cancer, often in a way dependent on patient, cancer type and treatment. Herein, we shall report on an update on platelet-cancer relationships, with a particular emphasis on how platelets might exert either a protective or a deleterious action in all steps of cancer progression. To this end, we will describe the impact of (i) platelet count, (ii) bioactive molecules secreted upon platelet activation, and (iii) microvesicle-derived miRNAs on cancer behavior. Potential explanations of conflicting results are also reported: Both intrinsic (heterogeneity in platelet-derived bioactive molecules with either inhibitory or stimulatory properties; features of cancer cell types, such as aggressiveness and/or tumour stage) and extrinsic (heterogeneous characteristics of cancer patients, study design and sample preparation) factors, together with other confounding elements, contribute to “the Janus face” of platelets in cancer. Given the difficulty to establish the univocal role of platelets in a tumor, a better understanding of their exact contribution is warranted, in order to identify an efficient therapeutic strategy for cancer management, as well as for better prevention, screening and risk assessment protocols.
Neurobiology Research in Parkinson’s Disease
Authors: Yasuhara Takao
Published: January 2020

In recent years, therapeutic strategies for Parkinson’s disease (PD) have been developing in many different ways [...]
The β-Blocker Carvedilol Prevented Ultraviolet-Mediated Damage of Murine Epidermal Cells and 3D Human Reconstructed Skin
Authors: Chen Mengbing , Liang Sherry , Shahid Ayaz , Andresen Bradley T. , Huang Ying
Published: January 2020

The β-blocker carvedilol prevents ultraviolet (UV)-induced skin cancer, but the mechanism is unknown. Since carvedilol possesses antioxidant activity, this study investigated whether carvedilol prevents oxidative photodamage of skin, a precursor event in skin carcinogenesis. The effects of carvedilol, metoprolol (a β-blocker without antioxidant property), and 4-hydroxycarbazole (4-OHC, a carvedilol synthesis intermediate and a free radical scavenger) were compared on UV- or H2O2-induced cell death and reactive oxygen species (ROS) production in murine epidermal JB6 P+ cells. Although carvedilol attenuated cell death, metoprolol and 4-OHC failed to show protective effects. As expected, increased cellular ROS induced by H2O2 or UV was abolished by carvedilol and 4-OHC, but not by metoprolol. Consistently, carvedilol attenuated the formation of UV-induced cyclobutane pyrimidine dimers (CPDs) and release of prostaglandin E2 in JB6 P+ cells. Carvedilol’s activity was further confirmed in full thickness 3D human reconstituted skin, where carvedilol attenuated UV-mediated epidermal thickening, the number of Ki-67 and p53 positive cells as well as CPD formation. Based on pathway-specific Polymerase Chain Reaction (PCR) Array analysis, carvedilol treatment in many cases normalized UV-induced expression changes in DNA repair genes. Thus, carvedilol’s photoprotective activity is not attributed to β-blockade or direct ROS-scavenging capacity, but likely via DNA repair regulation.
Enhanced Inflammation is a Marker for Risk of Post-Infarct Ventricular Dysfunction and Heart Failure
Authors: Świątkiewicz Iwona , Magielski Przemysław , Kubica Jacek , Zadourian Adena , DeMaria Anthony N. , Taub Pam R.
Published: January 2020

Acute ST-segment elevation myocardial infarction (STEMI) activates inflammation that can contribute to left ventricular systolic dysfunction (LVSD) and heart failure (HF). The objective of this study was to examine whether high-sensitivity C-reactive protein (CRP) concentration is predictive of long-term post-infarct LVSD and HF. In 204 patients with a first STEMI, CRP was measured at hospital admission, 24 h (CRP24), discharge (CRPDC), and 1 month after discharge (CRP1M). LVSD at 6 months after discharge (LVSD6M) and hospitalization for HF in long-term multi-year follow-up were prospectively evaluated. LVSD6M occurred in 17.6% of patients. HF hospitalization within a median follow-up of 5.6 years occurred in 45.7% of patients with LVSD6M vs. 4.9% without LVSD6M (p < 0.0001). Compared to patients without LVSD6M, the patients with LVSD6M had higher CRP24 and CRPDC and persistent CRP1M ≥ 2 mg/L. CRP levels were also higher in patients in whom LVSD persisted at 6 months (51% of all patients who had LVSD at discharge upon index STEMI) vs. patients in whom LVSD resolved. In multivariable analysis, CRP24 ≥ 19.67 mg/L improved the prediction of LVSD6M with an increased odds ratio of 1.47 (p < 0.01). Patients with LVSD6M who developed HF had the highest CRP during index STEMI. Elevated CRP concentration during STEMI can serve as a synergistic marker for risk of long-term LVSD and HF.
Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry
Authors: Sun Lue , Inaba Yohei , Kanzaki Norie , Bekal Mahesh , Chida Koichi , Moritake Takashi
Published: January 2020

Biodosimetry is a useful method for estimating personal exposure doses to ionizing radiation. Studies have identified metabolites in non-cellular biofluids that can be used as markers in biodosimetry. Levels of metabolites in blood cells may reflect health status or environmental stresses differentially. Here, we report changes in the levels of murine blood cell metabolites following exposure to X-rays in vivo. Levels of blood cell metabolites were measured by capillary electrophoresis time-of-flight mass spectrometry. The levels of 100 metabolites were altered substantially following exposure. We identified 2-aminobutyric acid, 2′-deoxycytidine, and choline as potentially useful markers of radiation exposure and established a potential prediction panel of the exposure dose using stepwise regression. Levels of blood cell metabolites may be useful biomarkers in estimating exposure doses during unexpected radiation incidents.
Salivary Immunoglobulin A Secretion and Polymeric Ig Receptor Expression in the Submandibular Glands Are Enhanced in Heat-Acclimated Rats
Authors: Matsuzaki Kentaro , Sugimoto Naotoshi , Islam Rafiad , Hossain Md Emon , Sumiyoshi Eri , Katakura Masanori , Shido Osamu
Published: January 2020

Salivary immunoglobulin A (IgA) plays a critical role in mucosal immunity. Chronic exposure to moderate heat induces heat acclimation, which modifies salivary functions. However, the changes in salivary IgA secretion in heat-acclimated rats are unclear. In this study, we investigated salivary IgA secretion and the expression of polymeric Ig receptor (pIgR), a key mediator of mucosal IgA secretion, in the submandibular glands (SMGs) of heat-acclimated rats. Following maintenance at an ambient temperature (Ta) of 24 ± 0.1 °C for 10 days, male Wistar rats were subjected to Ta of 32 ± 0.2 °C for 5 days (HE group) for heat acclimation or maintained at Ta of 24 ± 0.1°C (CN group). The rats were then anesthetized, pilocarpine (0.5 mg/kg) was intraperitoneally injected, and saliva was collected. Afterward, the SMGs and plasma were sampled. The salivary IgA concentration and IgA flow rate were significantly higher in the HE group than in the CN group. Similarly, SMG pIgR expression was significantly higher in HE rats. The levels of plasma cytokines, including interleukin (IL)-5, IL-6, and interferon-γ, were significantly greater in HE rats than in CN rats. Heat acclimation may enhance oral immunity through salivary IgA secretion and pIgR upregulation in the SMGs.
Increased Urinary 3-Mercaptolactate Excretion and Enhanced Passive Systemic Anaphylaxis in Mice Lacking Mercaptopyruvate Sulfurtransferase, a Model of Mercaptolactate-Cysteine Disulfiduria
Authors: Akahoshi Noriyuki , Minakawa Tatsuro , Miyashita Masashi , Sugiyama Uran , Saito Chihiro , Takemoto Rintaro , Honda Akihiro , Kamichatani Waka , Kamata Shotaro , Anan Yasumi , Ishii Isao
Published: January 2020

Mercaptopyruvate sulfurtransferase (Mpst) and its homolog thiosulfate sulfurtransferase (Tst = rhodanese) detoxify cyanide to thiocyanate. Mpst is attracting attention as one of the four endogenous hydrogen sulfide (H2S)/reactive sulfur species (RSS)-producing enzymes, along with cystathionine β-synthase (Cbs), cystathionine γ-lyase (Cth), and cysteinyl-tRNA synthetase 2 (Cars2). MPST deficiency was found in 1960s among rare hereditary mercaptolactate-cysteine disulfiduria patients. Mpst-knockout (KO) mice with enhanced liver Tst expression were recently generated as its model; however, the physiological roles/significances of Mpst remain largely unknown. Here we generated three independent germ lines of Mpst-KO mice by CRISPR/Cas9 technology, all of which maintained normal hepatic Tst expression/activity. Mpst/Cth-double knockout (DKO) mice were generated via crossbreeding with our previously generated Cth-KO mice. Mpst-KO mice were born at the expected frequency and developed normally like Cth-KO mice, but displayed increased urinary 3-mercaptolactate excretion and enhanced passive systemic anaphylactic responses when compared to wild-type or Cth-KO mice. Mpst/Cth-DKO mice were also born at the expected frequency and developed normally, but excreted slightly more 3-mercaptolactate in urine compared to Mpst-KO or Cth-KO mice. Our Mpst-KO, Cth-KO, and Mpst/Cth-DKO mice, unlike semi-lethal Cbs-KO mice and lethal Cars2-KO mice, are useful tools for analyzing the unknown physiological roles of endogenous H2S/RSS production.
Myoblast Migration and Directional Persistence Affected by Syndecan-4-Mediated Tiam-1 Expression and Distribution
Authors: Becsky Daniel , Gyulai-Nagy Szuzina , Balind Arpad , Horvath Peter , Dux Laszlo , Keller-Pinter Aniko
Published: January 2020

Skeletal muscle is constantly renewed in response to injury, exercise, or muscle diseases. Muscle stem cells, also known as satellite cells, are stimulated by local damage to proliferate extensively and form myoblasts that then migrate, differentiate, and fuse to form muscle fibers. The transmembrane heparan sulfate proteoglycan syndecan-4 plays multiple roles in signal transduction processes, such as regulating the activity of the small GTPase Rac1 (Ras-related C3 botulinum toxin substrate 1) by binding and inhibiting the activity of Tiam1 (T-lymphoma invasion and metastasis-1), a guanine nucleotide exchange factor for Rac1. The Rac1-mediated actin remodeling is required for cell migration. Syndecan-4 knockout mice cannot regenerate injured muscle; however, the detailed underlying mechanism is unknown. Here, we demonstrate that shRNA-mediated knockdown of syndecan-4 decreases the random migration of mouse myoblasts during live-cell microscopy. Treatment with the Rac1 inhibitor NSC23766 did not restore the migration capacity of syndecan-4 silenced cells; in fact, it was further reduced. Syndecan-4 knockdown decreased the directional persistence of migration, abrogated the polarized, asymmetric distribution of Tiam1, and reduced the total Tiam1 level of the cells. Syndecan-4 affects myoblast migration via its role in expression and localization of Tiam1; this finding may facilitate greater understanding of the essential role of syndecan-4 in the development and regeneration of skeletal muscle.
From HMI to HMIs: Towards an HMI Framework for Automated Driving
Authors: Bengler Klaus , Rettenmaier Michael , Fritz Nicole , Feierle Alexander
Published: January 2020

During automated driving, there is a need for interaction between the automated vehicle (AV) and the passengers inside the vehicle and between the AV and the surrounding road users outside of the car. For this purpose, different types of human machine interfaces (HMIs) are implemented. This paper introduces an HMI framework and describes the different HMI types and the factors influencing their selection and content. The relationship between these HMI types and their influencing factors is also presented in the framework. Moreover, the interrelations of the HMI types are analyzed. Furthermore, we describe how the framework can be used in academia and industry to coordinate research and development activities. With the help of the HMI framework, we identify research gaps in the field of HMI for automated driving to be explored in the future.
Information Needs and Visual Attention during Urban, Highly Automated Driving—An Investigation of Potential Influencing Factors
Authors: Feierle Alexander , Danner Simon , Steininger Sarah , Bengler Klaus
Published: January 2020

During highly automated driving, the passenger is allowed to conduct non-driving related activities (NDRA) and no longer has to act as a fallback at the functional limits of the driving automation system. Previous research has shown that at lower levels of automation, passengers still wish to be informed about automated vehicle behavior to a certain extent. Due to the aim of the introduction of urban automated driving, which is characterized by high complexity, we investigated the information needs and visual attention of the passenger during urban, highly automated driving. Additionally, there was an investigation into the influence of the experience of automated driving and of NDRAs on these results. Forty participants took part in a driving simulator study. As well as the information presented on the human–machine interface (system status, navigation information, speed and speed limit), participants requested information about maneuvers, reasons for maneuvers, environmental settings and additional navigation data. Visual attention was significantly affected by the NDRA, while the experience of automated driving had no effect. Experience and NDRA showed no significant effect on the need for information. Differences in information needs seem to be due to the requirements of the individual passenger, rather than the investigated factors.
Spectral Normalization for Domain Adaptation
Authors: Zhao Liquan , Liu Yan
Published: January 2020

The transfer learning method is used to extend our existing model to more difficult scenarios, thereby accelerating the training process and improving learning performance. The conditional adversarial domain adaptation method proposed in 2018 is a particular type of transfer learning. It uses the domain discriminator to identify which images the extracted features belong to. The features are obtained from the feature extraction network. The stability of the domain discriminator directly affects the classification accuracy. Here, we propose a new algorithm to improve the predictive accuracy. First, we introduce the Lipschitz constraint condition into domain adaptation. If the constraint condition can be satisfied, the method will be stable. Second, we analyze how to make the gradient satisfy the condition, thereby deducing the modified gradient via the spectrum regularization method. The modified gradient is then used to update the parameter matrix. The proposed method is compared to the ResNet-50, deep adaptation network, domain adversarial neural network, joint adaptation network, and conditional domain adversarial network methods using the datasets that are found in Office-31, ImageCLEF-DA, and Office-Home. The simulations demonstrate that the proposed method has a better performance than other methods with respect to accuracy.
Association of Trimethylamine, Trimethylamine N-oxide, and Dimethylamine with Cardiovascular Risk in Children with Chronic Kidney Disease
Authors: Hsu Chien-Ning , Chang-Chien Guo-Ping , Lin Sufan , Hou Chih-Yao , Lu Pei-Chen , Tain You-Lin
Published: January 2020

Chronic kidney disease (CKD) is associated with high risk for cardiovascular disease (CVD). Gut microbiota-dependent metabolites trimethylamine (TMA), trimethylamine N-oxide (TMAO), and dimethylamine (DMA) have been linked to CKD and CVD. We examined whether these methylamines are correlated with cardiovascular risk in CKD children. A total of 115 children and adolescents with CKD stage G1–G4 were enrolled in this cross-sectional study. Children with CKD stage G2–G4 had higher plasma levels of DMA, TMA, and TMAO, but lower urinary levels of DMA and TMAO than those with CKD stage G1. Up to 53% of CKD children and adolescents had blood pressure (BP) abnormalities on 24-h ambulatory BP monitoring (ABPM). Plasma TMA and DMA levels inversely associated with high BP load as well as estimated glomerular filtration rate (eGFR). Additionally, CKD children with an abnormal ABPM profile had decreased abundance of phylum Cyanobacteria, genera Subdoligranulum, Faecalibacterium, Ruminococcus, and Akkermansia. TMA and DMA are superior to TMAO when related to high BP load and other CV risk factors in children and adolescents with early-stage CKD. Our findings highlight that gut microbiota-dependent methylamines are related to BP abnormalities and CV risk in pediatric CKD. Further studies should determine whether these microbial markers can identify children at risk for CKD progression.
Fenofibrate Facilitates Post-Active Tuberculosis Infection in Macrophages and is Associated with Higher Mortality in Patients under Long-Term Treatment
Authors: Liu Ching-Lung , Lu Yen-Ta , Tsai I-Fan , Wu Ling-Chiao , Chien Wu-Chien , Chung Chi-Hsiang , Ma Kuo-Hsing
Published: January 2020

Background: Mycobacterium tuberculosis (Mtb) is an intracellular pathogen that infects and persists in macrophages. This study aimed to investigate the effects of long-term fenofibrate treatment in patients with tuberculosis (TB), and the intracellular viability of Mtb in human macrophages. Methods: Epidemiological data from the National Health Insurance Research Database of Taiwan were used to present outcomes of TB patients treated with fenofibrate. In the laboratory, we assessed Mtb infection in macrophages treated with or without fenofibrate. Mtb growth, lipid accumulation in macrophages, and expression of transcriptional genes were examined. Results: During 11 years of follow-up, TB patients treated with fenofibrate presented a higher risk of mortality. Longer duration of fenofibrate use was associated with a significantly higher risk of mortality. Treatment with fenofibrate significantly increased the number of bacilli in human macrophages in vitro. Fenofibrate did not reduce, but induced an increasing trend in the intracellular lipid content of macrophages. In addition, dormant genes of Mtb, icl1, tgs1, and devR, were markedly upregulated in response to fenofibrate treatment. Our results suggest that fenofibrate may facilitate intracellular Mtb persistence. Conclusions: Our data shows that long-term treatment with fenofibrate in TB patients is associated with a higher mortality. The underlying mechanisms may partly be explained by the upregulation of Mtb genes involved in lipid metabolism, enhanced intracellular growth of Mtb, and the ability of Mtb to sustain a nutrient-rich reservoir in human macrophages, observed during treatment with fenofibrate.
Molecular Bases of Neurodegeneration and Cognitive Decline, the Major Burden of Sanfilippo Disease
Authors: Heon-Roberts Rachel , Nguyen Annie L. A. , Pshezhetsky Alexey V.
Published: January 2020

The mucopolysaccharidoses (MPS) are a group of diseases caused by the lysosomal accumulation of glycosaminoglycans, due to genetic deficiencies of enzymes involved in their degradation. MPS III or Sanfilippo disease, in particular, is characterized by early-onset severe, progressive neurodegeneration but mild somatic involvement, with patients losing milestones and previously acquired skills as the disease progresses. Despite being the focus of extensive research over the past years, the links between accumulation of the primary molecule, the glycosaminoglycan heparan sulfate, and the neurodegeneration seen in patients have yet to be fully elucidated. This review summarizes the current knowledge on the molecular bases of neurological decline in Sanfilippo disease. It emerges that this deterioration results from the dysregulation of multiple cellular pathways, leading to neuroinflammation, oxidative stress, impaired autophagy and defects in cellular signaling. However, many important questions about the neuropathological mechanisms of the disease remain unanswered, highlighting the need for further research in this area.
Title: Human Serum/Plasma Glycoprotein Analysis by 1H-NMR, an Emerging Method of Inflammatory Assessment
Authors: Fuertes-Martín Rocío , Correig Xavier , Vallvé Joan-Carles , Amigó Núria
Published: January 2020

Several studies suggest that variations in the concentration of plasma glycoproteins can influence cellular changes in a large number of diseases. In recent years, proton nuclear magnetic resonance (1H-NMR) has played a major role as an analytical tool for serum and plasma samples. In recent years, there is an increasing interest in the characterization of glycoproteins through 1H-NMR in order to search for reliable and robust biomarkers of disease. The objective of this review was to examine the existing studies in the literature related to the study of glycoproteins from an analytical and clinical point of view. There are currently several techniques to characterize circulating glycoproteins in serum or plasma, but in this review, we focus on 1H-NMR due to its great robustness and recent interest in its translation to the clinical setting. In fact, there is already a marker in H-NMR representing the acetyl groups of the glycoproteins, GlycA, which has been increasingly studied in clinical studies. A broad search of the literature was performed showing a general consensus that GlycA is a robust marker of systemic inflammation. The results also suggested that GlycA better captures systemic inflammation even more than C-reactive protein (CRP), a widely used classical inflammatory marker. The applications reviewed here demonstrated that GlycA was potentially a key biomarker in a wide range of diseases such as cancer, metabolic diseases, cardiovascular risk, and chronic inflammatory diseases among others. The profiling of glycoproteins through 1H-NMR launches an encouraging new paradigm for its future incorporation in clinical diagnosis.
New Theoretical Model of the Irradiance Distribution in Water from a Unidirectional Point Source
Authors: Dolin Lev S.
Published: January 2020

Formulas are presented for calculating the irradiance field, which is formed in a turbid medium with a narrow scattering phase function and homogeneous optical properties when an infinitely narrow light beam passes through it. The calculations are based on a new mathematical model of the stationary radiation field of an omnidirectional point source and relationships enabling one to represent the irradiance distribution in a continuous or modulated light beam through this field. The obtained formulas, in contrast to the previously known ones, permit taking into account the temporal spreading of a pulsed light beam in the sea without a significant decrease in the accuracy of describing its spatial structure.
Role of Social Norms in Natural Resource Management: The Case of the Communal Land Distribution Program in Northern Ethiopia
Authors: Oniki Shunji , Berhe Melaku , Negash Teklay
Published: January 2020

The increasing population pressure in the rural areas of Sub-Saharan Africa has caused land degradation as well as an increase in the number of landless farmers. To promote a conservation-oriented utilization of communal lands and increase the livelihood of poor farmers, the Ethiopian government introduced a program to distribute less-utilized communal lands to landless farmers. This study identified the social norms related to natural resource conservation that affect the participation in this program. Using data from 477 farmer households in northern Ethiopia, we estimated probit models with endogenous regressors for the determinants of social norms and their impacts on program participation. The results show that social norms related to conservation positively affect program participation. Regarding policy implication of the findings, an intervention to improve the social norms of local farmers leads to sustainable resource conservation without reducing intrinsic motivation of the local people. A conservation-oriented utilization of the communal lands would be more effective if the land distribution program was accompanied by other programs to improve the social norms in the villages.
Assessing Changes in Ecosystem Service Values over 1985–2050 in Response to Land Use and Land Cover Dynamics in Abaya-Chamo Basin, Southern Ethiopia
Authors: Woldeyohannes Ashebir , Cotter Marc , Biru Wubneshe , Kelboro Girma
Published: January 2020

This study evaluated the effect of Land Use and Land Cover (LULC) dynamics on the value of ecosystem services in Abaya-Chamo basin over 1985–2050. The main objectives of the study were to estimate the value of ecosystem services of Abaya-Chamo basin using local and global ecosystem service value coefficients, assess how it changes over time, and develop tools to inform policy and public decision-making to protect lands and waters in the region. The study utilized observed (1985 and 2010) and predicted (2030 and 2050) LULC datasets and ecosystem service value coefficients obtained from publications in peer-reviewed scientific journals. The results indicated that the total ecosystem service value of Abaya-Chamo basin was 12.13 billion USD in 1985 and 12.45 billion USD in 2010. The value is predicted to increase to 12.47 billion USD by the year 2050, which is 2.84% (344.5 million USD) higher than the total value of ecosystem services of the basin in 1985. Although the total ecosystem service value of the basin showed a slight increase over the study period, it was observed that the total value of services obtained from natural ecosystems is expected to decline by 36.24% between 1985 and 2050. The losses of services obtained from natural ecosystems, such as water regulation and erosion control, are major concern as the consequence has already been reported in the basin in the form of reduced water quality and productivity of the lakes due to an increased soil erosion and sediment transport in the basin. Therefore, special attention should be given to the rehabilitation of degraded ecosystems and the protection of remaining natural vegetation and water bodies to enhance natural capital and ecosystem services in the basin. A large-scale dissemination of eco-agricultural land use practices, which provide multiple ecosystem services (such as agroforestry and heterogeneous agricultural areas) in the basin, needs to be considered in the future.