bluuebunny/crossref_metadata_2025 huggingface dataset:
Can the Unified Theory of Acceptance and Use of Technology (UTAUT) Help Explain Subjective Well-Being in Senior Citizens due to Gateball Participation?
Authors: Hsu Chia-Chien , Sandford Brian , Ling Chia-Ju , Lin Ching-Torng
Published: August 2021

Promoting successful aging strategies through well-reasoned caregiving programs is, and should be, one of the main objectives of many government policies and their implementing agencies. Well-being has been considered an important indicator of successful aging. Leisure is a key life domain and a core ingredient for overall well-being. Yet, within well-being research, few studies have made the connection between leisure participation as accepted behavior and subjective well-being in senior citizens. This study proposed to examine the applicability of the Unified Theory of Acceptance and Use of Technology (UTAUT) in explaining senior citizens’ decision-making processes in terms of leisure participation behavior and the effect of such behavioral engagement on subjective well-being. The respondents were senior citizens in Taiwan who played gateball and were aged 60 years or older. A total of 595 usable responses were obtained and used to answer the research question. The empirical results indicate that performance expectancy, social influence, and facilitating conditions are positively and significantly related to senior citizens’ gateball participation behavior. In addition, gateball participation behavior had a positive and significant effect on respondents’ subjective well-being. The results of this study not only extend the application of UTAUT in terms of participation behavior in leisure activities, but also can provide gateball associations and government entities a theoretical model for developing and promoting gateball programs which serve or involve the elderly, as well as helping older adults to pursue satisfactory levels of wellbeing.
SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line
Authors: Zupin Luisa , Fontana Francesco , Gratton Rossella , Milani Margherita , Clemente Libera , Pascolo Lorella , Ruscio Maurizio , Crovella Sergio
Published: August 2021

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is mainly transmitted through respiratory droplets from positive subjects to susceptible hosts or by direct contact with an infected individual. Our study focuses on the in vitro minimal time of viral absorption as well as the minimal quantity of virus able to establish a persistent infection in Vero E6 cells. We observed that 1 min of in vitro virus exposure is sufficient to generate a cytopathic effect in cells after 7 days of infection, even at a multiplicity of infection (MOI) value of 0.01. Being aware that our findings have been obtained using an in vitro cellular model, we demonstrated that short-time exposures and low viral concentrations are able to cause infection, thus opening questions about the risk of SARS-CoV-2 transmissibility even following short contact times.
Self-Reported Diabetes Mellitus and Tooth Extraction Due to Periodontal Disease and Dental Caries in the Japanese Population
Authors: Suzuki Seitaro , Sugihara Naoki , Kamijo Hideyuki , Morita Manabu , Kawato Takayuki , Tsuneishi Midori , Kobayashi Keita , Hasuike Yoshihiro , Sato Tamotsu
Published: August 2021

Diabetes mellitus is closely related to oral health. We aimed to determine the relationship between diabetes mellitus and tooth extraction due to periodontal disease and dental caries. Japan’s second nationwide survey data collected from 4 June to 10 June 2018 was used to identify reasons for tooth extraction among patients aged > 40 years. General dentists collected information on patients who underwent tooth extraction procedures, and the presence of diabetes mellitus was determined through interviews. Multivariable logistic regression was performed to investigate the relationship between diabetes mellitus and the reasons for tooth extraction, including periodontal disease and dental caries. In total, 2345 dentists responded to the survey (response rate 44.8%). We analyzed data on 4625 extracted teeth from 3750 patients (1815 males and 1935 females). Among patients with self-reported diabetes mellitus, 55.4% had extractions due to periodontal disease compared to 46.7% of such extractions among those without self-reported diabetes mellitus. Self-reported diabetes mellitus was significantly associated with tooth extraction due to periodontal disease. No significant differences were observed in dental caries according to self-reported diabetes mellitus status. This study provides further evidence of a significant association between diabetes mellitus and tooth extraction due to periodontal disease.
Self-Control, Consideration of Future Consequences, and Internet Addiction among Chinese Adolescents: The Moderating Effect of Deviant Peer Affiliation
Authors: Li Jingjing , Chen Yanhan , Lu Jiachen , Li Weidong , Yu Chengfu
Published: August 2021

Although a large number of studies have indicated that self-control was an important predictive factor for adolescent internet addiction, the moderating and mediating mechanisms underlying this relationship remain unclear. To address this research gap, the present study, according to social learning theory and the organism–environment interaction theory, tested whether consideration of future consequences mediated the relation between self-control and adolescent internet addiction and whether deviant peer affiliation moderated the relationship between consideration of future consequences and internet addiction. Using longitudinal tracking (two-time points and the interval is 6 months). Three middle schools in Guangzhou were randomly selected. The participants were 1182 students ranging in age from 12 to 15 years (average age: 14.16, SD = 1.29) from three middle schools in Guangzhou (651 boys and 531 girls) in Guangdong Province. Results showed that consideration of future consequences mediated the relationship between self-control and internet addiction. Furthermore, the relationship between future consequence consideration and internet addiction was moderated by deviant peer affiliation. These findings highlighted the potential mediating role of consideration of future consequences in linking self-control to adolescent internet addiction. We also found high deviant peer affiliation weakens the protective effect of future consequence consideration on Internet addiction. This study may provide support for adolescent Internet addiction prevention and have some educational implications.
Dynamical Analysis of Universal Masking on the Pandemic
Authors: Tay Brandon Kaiheng , Roby Carvalho Andrea , Wu Jodi Wenjiang , Tan Da Yang
Published: August 2021

We investigate the impact of the delay in compulsory mask wearing on the spread of COVID-19 in the community, set in the Singapore context. By using modified SEIR-based compartmental models, we focus on macroscopic population-level analysis of the relationships between the delay in compulsory mask wearing and the maximum infection, through a series of scenario-based analysis. Our analysis suggests that collective masking can meaningfully reduce the transmission of COVID-19 in the community, but only if implemented within a critical time window of approximately before 80–100 days delay after the first infection is detected, coupled with strict enforcement to ensure compliance throughout the duration. We also identify a delay threshold of about 100 days that results in masking enforcement having little significant impact on the Maximum Infected Values. The results therefore highlight the necessity for rapid implementation of compulsory mask wearing to curb the spread of the pandemic.
Assessment of the Psychophysical Sphere and Functional Status of Women Aged 75–90 Living Alone and in Nursing Homes
Authors: Traczyk Justyna , Dębiec-Bąk Agnieszka , Skrzek Anna , Stefańska Małgorzata
Published: August 2021

Aim: To assess the psychophysical sphere and functional status of women aged 75–90 living alone and in nursing homes. Methods: 23 women living in nursing homes (NH) and 20 living alone (HOME) underwent the following tests: Up and Go Test, Chair Stand Test, assessment of daily physical activity levels using pedometers, Mini-Mental State Examination, Groningen Activity Restriction Scale, Geriatric Depression Scale, and WHOQOL-Bref. Results: It was shown that the subjects living by themselves performed a greater amount of daily physical activity, although there was not significant difference between the two groups in the Up and Go Test result. There were not statistically significant differences in the self-assessment of the mental sphere, but significant differences were found in the self-evaluation of the physical sphere. In the NH group, subjects with and without depression did not differ in terms of the amount of daily physical activity and functional test results. Women with depression from the HOME group were less physically active and had worse functional fitness. Conclusions: Women living alone performed a greater amount of daily physical activity, but the functional status of women in both groups did not differ in a statistically significant way. The groups didn’t differ statistically significantly in terms of psychological self-assessment.
Greening Wine Exports? Changes in the Carbon Footprint of Spanish Wine Exports
Authors: Carrasco Inmaculada , Castillo-Valero Juan Sebastián , Córcoles Carmen , Carchano Marcos
Published: August 2021

Spain is one of the leading wine-producing and -exporting countries and has traditionally been dominant in trade and world production in the sector. In an increasingly changing context, in which worldwide wine exports are growing exponentially, it is essential to study their impact on climate change as the transport of goods generates a significant volume of greenhouse gas emissions. The aim of this work, then, was to analyse the variation in the carbon footprint generated by Spanish wine exports between 2011 and 2016. To this end, a multi-regional input–output (MRIO) model was used, showing that the emissions associated with wine operations have increased less than exports, which might suggest that sustainable growth has been included as a goal in the wine supply chain. The methodology used has the advantage of allowing the calculation of direct and indirect emissions. At the same time, the results can provide relevant information to practitioners and policymakers due to the expected evolution of European environmental regulations and trades, in terms of carbon footprint.
Cervicovaginal Microbiome after Cervical Intraepithelial Neoplasia Treatment. A Protocol for Systematic Review and Meta-Analysis
Authors: Janicka-Kośnik Marta , Sarecka-Hujar Beata , Jakiel Grzegorz , Słabuszewska-Jóźwiak Aneta
Published: August 2021

(1) Background: The microbiome consists of microorganisms from various kingdoms with numerous physical and chemical properties Lactobacillus species constitute the highest percentage of healthy cervical and vaginal microbiota. Dysbiosis may cause adverse outcomes, e.g., bacterial vaginosis, pelvic inflammatory disease and pregnancy complications. The cervicovaginal microbiome might contribute to the development of a persistent HPV infection—the main risk factor of cervical cancer—and influence progression to malignancy The aim is to perform a systematic review of current literature and a meta-analysis regarding microbiome changes after cervical intraepithelial neoplasia treatment. (2) Methods: We will search PubMed, Scopus, Google Scholar and Embase Database and trace citations in the reference sections. Randomized and non-randomized controlled studies, case–control and cohort studies published between January 2000 and May 2021 will be included in the study protocol. The following keywords will be used: ‘microbiome’, ‘vaginal microbiome’, ‘cervical microbiome’, ‘cervical neoplasia treatment’, ’conization’, ‘electroconization’, and ‘electrosurgical treatment’. Statistical analyses will be performed using RevMan 5.4. (3) Results: The results will be published as a peer-reviewed article. (4) Conclusions: The study will show which forms of intraepithelial neoplasia treatment change the cervicovaginal microbiome. Finding the best form of treatment by studying the cervicovaginal microbiome after various forms of treatment is essential. Patients would benefit not only from the treatment of the initial disease but also the management of dysbiosis, which might underlie other pathologies.
A Systematic Review of E-Waste Generation and Environmental Management of Asia Pacific Countries
Authors: Andeobu Lynda , Wibowo Santoso , Grandhi Srimannarayana
Published: August 2021

Due to the rapid increase in the use of electrical and electronic equipment (EEE) worldwide, e-waste has become a critical environmental issue for many governments around the world. Several studies have pointed out that failure to adopt appropriate recycling practices for e-waste may cause environmental disasters and health concerns to humans due to the presence of hazardous materials. This warrants the need for a review of the existing processes of e-waste management. In view of the growing e-waste generation in the Asia Pacific region and the importance of e-waste management, this study critically reviews previous research on e-waste generation and management practices of major e-waste producing nations (Australia, China, India, Indonesia, and Malaysia) in the Asia Pacific region, provides an overview of progress made and identifies areas for improvement. To fulfil the aims of this research, previous studies from 2005 to 2020 are collected from various databases. Accordingly, this study focuses on e-waste generation and environmental management of these countries. This study found that e-waste management practices of the selected countries need to be enhanced and recommends several best practices for effectively managing e-waste.
Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product
Authors: Pires Eleomar de O. , Pereira Eliana , Carocho Márcio , Pereira Carla , Dias Maria Inês , Calhelha Ricardo C. , Ćirić Ana , Soković Marina , Garcia Carolina C. , Ferreira Isabel C. F. R. , Caleja Cristina , Barros Lillian
Published: August 2021

Flowers of the genus Impatiens are classified as edible; however, their inclusion in the human diet is not yet a common practice. Its attractive colours have stirred great interest by the food industry. In this sense, rose (BP) and orange (BO) I. balsamina flowers were nutritionally studied, followed by an in-depth chemical study profile. The non-anthocyanin and anthocyanin profiles of extracts of both flower varieties were also determined by high-performance liquid chromatography coupled to a diode array and mass spectrometry detector (HPLC-DAD-ESI/MS). The results demonstrated that both varieties presented significant amounts of phenolic compounds, having identified nine non-anthocyanin compounds and 14 anthocyanin compounds. BP extract stood out in its bioactive properties (antioxidant and antimicrobial potential) and was selected for incorporation in “bombocas” filling. Its performance as a colouring ingredient was compared with the control formulations (white filling) and with E163 (anthocyanins) colorant. The incorporation of the natural ingredient did not cause changes in the chemical and nutritional composition of the product; and although the colour conferred was lighter than presented by the formulation with E163 (suggesting a more natural aspect), the higher antioxidant activity could meet the expectations of the current high-demand consumer.
Improving the Sexual Health of Young People (under 25) in High-Risk Populations: A Systematic Review of Behavioural and Psychosocial Interventions
Authors: Brown Ellie , Lo Monaco Samantha , O’Donoghue Brian , Nolan Hayley , Hughes Elizabeth , Graham Melissa , Simmons Magenta , Gray Richard
Published: August 2021

Background: Ensuring young people experience good sexual health is a key public health concern, yet some vulnerable groups of young people remain at higher risk of poor sexual health. These individuals require additional support to achieve good sexual health but the best way to provide this remains needs to be better understood. Methods: We searched for randomised controlled trials of behavioural and psychosocial interventions aimed at promoting sexual health in high-risk young populations. Outcomes of interest were indicators of sexual health (e.g., condom use, attitudes to contraception, knowledge of risk). Participants were under 25 years old and in one of the following high-risk groups: alcohol and other drug use; ethnic minority; homeless; justice-involved; LGBTQI+; mental ill-health; or out-of-home care. Results: Twenty-eight papers from 26 trials met our inclusion criteria, with all but one conducted in North America. Condom use was the most frequently reported outcome measure along with knowledge and attitudes towards sexual health but considerable differences in measures used made comparisons across studies difficult. Change in knowledge and attitudes did not consistently result in long-term change in behaviours. Conclusions: There remains a dearth of research undertaken outside of North America across all high-risk groups of young people. Future interventions should address sexual health more broadly than just the absence of negative biological outcomes, with LGBTQI+, homeless and mental ill-health populations targeted for such work. An international consensus on outcome measures would support the research field going forward, making future meta-analyses possible.
Vision in Futsal Players: Coordination and Reaction Time
Authors: Nascimento Henrique , Alvarez-Peregrina Cristina , Martinez-Perez Clara , Sánchez-Tena Miguel Ángel
Published: August 2021

Background: Coordination and reaction time are relevant aspects of a sport’s competitive performance within teams. The aim of this study was to explore if a group of futsal players, in a laboratory context, would present better results from actions where vision is prevalent compared to a control group without contact with futsal or any other sport. Methods: The digital system of the COI- SV software was used; six tests were selected, related to coordination (“Eye/hand coordination”; “Coordination and identification”) and reaction time (“Anticipation Time”; “Peripheral response”; “reaction time”; “Visual memory”). Results: Of all the tests performed, only in the anticipation time test did the futsal players obtain better results than the control group. The average time of the failures was lower in relation to the control group. In the others, no differences were found between the two groups. Conclusions: The futsal players did not perform better than the control group in most of the tests carried out, except in the “anticipation time”. Therefore, visual training maybe necessary to improve visual skills and sports performance.
Telemonitoring of Real-World Health Data in Cardiology: A Systematic Review
Authors: Kinast Benjamin , Lutz Matthias , Schreiweis Björn
Published: August 2021

Background: New sensor technologies in wearables and other consumer health devices open up promising opportunities to collect real-world data. As cardiovascular diseases remain the number one reason for disease and mortality worldwide, cardiology offers potent monitoring use cases with patients in their out-of-hospital daily routines. Therefore, the aim of this systematic review is to investigate the status quo of studies monitoring patients with cardiovascular risks and patients suffering from cardiovascular diseases in a telemedical setting using not only a smartphone-based app, but also consumer health devices such as wearables and other sensor-based devices. Methods: A literature search was conducted across five databases, and the results were examined according to the study protocols, technical approaches, and qualitative and quantitative parameters measured. Results: Out of 166 articles, 8 studies were included in this systematic review; these cover interventional and observational monitoring approaches in the area of cardiovascular diseases, heart failure, and atrial fibrillation using various app, wearable, and health device combinations. Conclusions: Depending on the researcher’s motivation, a fusion of apps, patient-reported outcome measures, and non-invasive sensors can be orchestrated in a meaningful way, adding major contributions to monitoring concepts for both individual patients and larger cohorts.
Influential Factor Detection for Tourism on the Qinghai-Tibet Plateau Based on Social Media Data
Authors: Hu Lei , Xu Jun , Bao Chao , Pei Tao
Published: August 2021

Tourism is playing an important role in the economic development of the Qinghai-Tibet Plateau (QTP). To better develop tourism in this region, the spatial heterogeneity of influencing factors on tourism needs to be studied. Using the spatial distribution of tourism potential from social media data, this paper analyzes the influencing factors of tourism on the QTP from the perspective of spatial heterogeneity. We extract microblogs related to travel topics connected to the QTP in 2017 from Sina Weibo to capture tourism potential. Then, factors considered from six aspects (tourism resources, amenities, transportation, geography, population, and the economy) are selected, and a geographic detector (Geodetector) is employed to detect the explanatory power of these factors for tourism potential. The results indicate different influential tourism factors in Qinghai and Tibet. In Qinghai, the main factors are hotels, tourist attractions, and road network density, and the explanatory power of the factors mainly comes from eastern and western Qinghai. In Tibet, the main factors are road network density, regional GDP (Gross Domestic Product), and urban land. It is suggested that tourism in the central region of Qinghai can be improved by enhancing the publicity and utilization of tourism resources, and Tibet should enhance tourism resource utilization and improve tourism amenities and infrastructure.
Genome-Wide Identification and Characterization of KNOTTED-Like Homeobox (KNOX) Homologs in Garlic (Allium sativum L.) and Their Expression Profilings Responding to Exogenous Cytokinin and Gibberellin
Authors: Zhang Siyu , Pan Yupeng , Zhi Chengchen , Zheng Yujie , Wang Xi’ao , Li Xiaxia , Cheng Zhihui
Published: August 2021

Garlic (Allium sativum L.) is an important vegetable and is cultivated and consumed worldwide for its economic and medicinal values. Garlic cloves, the major reproductive and edible organs, are derived from the axillary meristems. KNOTTED-like homeobox (KNOX) proteins, such as SHOOT MERISTEM-LESS (STM), play important roles in axillary meristem formation and development. However, the KNOX proteins in garlic are still poorly known. Here, 10 AsKNOX genes, scattered on 5 of the 8 chromosomes, were genome-wide identified and characterized based on the newly released garlic genome. The typical conserved domains of KNOX proteins were owned by all these 10 AsKNOX homologs, which were divided into two Classes (Class I and Class II) based on the phylogenetic analysis. Prediction and verification of the subcellular localizations revealed the diverse subcellular localization of these 10 AsKNOX proteins. Cis-element prediction, tissue expression analysis, and expression profilings in responding to exogenous GA3 and 6-BA showed the potential involvement of AsKNOX genes in the gibberellin and cytokinin signaling pathways. Overall, the results of this work provided a better understanding of AsKNOX genes in garlic and laid an important foundation for their further functional studies.
Oral Microbiota Features in Subjects with Down Syndrome and Periodontal Diseases: A Systematic Review
Authors: Contaldo Maria , Lucchese Alberta , Romano Antonio , Della Vella Fedora , Di Stasio Dario , Serpico Rosario , Petruzzi Massimo
Published: August 2021

Down syndrome (DS) is a genetic disorder associated with early-onset periodontitis and other periodontal diseases (PDs). The present work aimed to systematically review the scientific literature reporting studies in vivo on oral microbiota features in subjects with DS and related periodontal health and to highlight any correlation and difference with subjects not affected by DS, with and without PDs. PubMed, Web of Science, Scopus and Cochrane were searched for relevant studies in May 2021. The participants were subjects affected by Down syndrome (DS) with and without periodontal diseases; the study compared subjects with periodontal diseases but not affected by DS, and DS without periodontal diseases; the outcomes were the differences in oral microbiota/periodontopathogen bacterial composition among subjects considered; the study design was a systematic review. Study quality was assessed with risk of bias in non-randomized studies of interventions (ROBINS-I). Of the 954 references retrieved, 26 studies were considered. The conclusions from the qualitative assessment of the papers revealed an increasing knowledge over the last years of the microbiota associated with DS and their periodontal diseases, in comparison with healthy subjects and subjects with other kinds of mental disabilities. Few data have emerged on the mycobiome and virobiome of DS, hence, further investigations are still necessary.
Implementation of a Non-Thermal Atmospheric Pressure Plasma for Eradication of Plant Pathogens from a Surface of Economically Important Seeds
Authors: Motyka-Pomagruk Agata , Dzimitrowicz Anna , Orlowski Jakub , Babinska Weronika , Terefinko Dominik , Rychlowski Michal , Prusinski Michal , Pohl Pawel , Lojkowska Ewa , Jamroz Piotr , Sledz Wojciech
Published: August 2021

Plant pathogenic bacteria cause significant economic losses in the global food production sector. To secure an adequate amount of high-quality nutrition for the growing human population, novel approaches need to be undertaken to combat plant disease-causing agents. As the currently available methods to eliminate bacterial phytopathogens are scarce, we evaluated the effectiveness and mechanism of action of a non-thermal atmospheric pressure plasma (NTAPP). It was ignited from a dielectric barrier discharge (DBD) operation in a plasma pencil, and applied for the first time for eradication of Dickeya and Pectobacterium spp., inoculated either on glass spheres or mung bean seeds. Furthermore, the impact of the DBD exposure on mung bean seeds germination and seedlings growth was estimated. The observed bacterial inactivation rates exceeded 3.07 logs. The two-minute DBD exposure stimulated by 3–4% the germination rate of mung bean seeds and by 13.4% subsequent early growth of the seedlings. On the contrary, a detrimental action of the four-minute DBD subjection on seed germination and early growth of the sprouts was noted shortly after the treatment. However, this effect was no longer observed or reduced to 9.7% after the 96 h incubation period. Due to the application of optical emission spectrometry (OES), transmission electron microscopy (TEM), and confocal laser scanning microscopy (CLSM), we found that the generated reactive oxygen and nitrogen species (RONS), i.e., N2, N2+, NO, OH, NH, and O, probably led to the denaturation and aggregation of DNA, proteins, and ribosomes. Furthermore, the cellular membrane disrupted, leading to an outflow of the cytoplasm from the DBD-exposed cells. This study suggests the potential applicability of NTAPPs as eco-friendly and innovative plant protection methods.
Influence of the Aryl Hydrocarbon Receptor Activating Environmental Pollutants on Autism Spectrum Disorder
Authors: Dhulkifle Hevna , Agouni Abdelali , Zeidan Asad , Al-Kuwari Mohammed Saif , Parray Aijaz , Tolefat Mohamed , Korashy Hesham M.
Published: August 2021

Autism spectrum disorder (ASD) is an umbrella term that includes many different disorders that affect the development, communication, and behavior of an individual. Prevalence of ASD has risen exponentially in the past couple of decades. ASD has a complex etiology and traditionally recognized risk factors only account for a small percentage of incidence of the disorder. Recent studies have examined factors beyond the conventional risk factors (e.g., environmental pollution). There has been an increase in air pollution since the beginning of industrialization. Most environmental pollutants cause toxicities through activation of several cellular receptors, such as the aryl hydrocarbon receptor (AhR)/cytochrome P450 (CYPs) pathway. There is little research on the involvement of AhR in contributing to ASD. Although a few reviews have discussed and addressed the link between increased prevalence of ASD and exposure to environmental pollutants, the mechanism governing this effect, specifically the role of AhR in ASD development and the molecular mechanisms involved, have not been discussed or reviewed before. This article reviews the state of knowledge regarding the impact of the AhR/CYP pathway modulation upon exposure to environmental pollutants on ASD risk, incidence, and development. It also explores the molecular mechanisms involved, such as epigenesis and polymorphism. In addition, the review explores possible new AhR-mediated mechanisms of several drugs used for treatment of ASD, such as sulforaphane, resveratrol, haloperidol, and metformin.
Unraveling the Relevance of ARL GTPases in Cutaneous Melanoma Prognosis through Integrated Bioinformatics Analysis
Authors: Brito Cheila , Costa-Silva Bruno , Barral Duarte C. , Pojo Marta
Published: August 2021

Cutaneous melanoma (CM) is the deadliest skin cancer, whose molecular pathways underlying its malignancy remain unclear. Therefore, new information to guide evidence-based clinical decisions is required. Adenosine diphosphate (ADP)-ribosylation factor-like (ARL) proteins are membrane trafficking regulators whose biological relevance in CM is undetermined. Here, we investigated ARL expression and its impact on CM prognosis and immune microenvironment through integrated bioinformatics analysis. Our study found that all 22 ARLs are differentially expressed in CM. Specifically, ARL1 and ARL11 are upregulated and ARL15 is downregulated regardless of mutational frequency or copy number variations. According to TCGA data, ARL1 and ARL15 represent independent prognostic factors in CM as well as ARL11 based on GEPIA and OncoLnc. To investigate the mechanisms by which ARL1 and ARL11 increase patient survival while ARL15 reduces it, we evaluated their correlation with the immune microenvironment. CD4+ T cells and neutrophil infiltrates are significantly increased by ARL1 expression. Furthermore, ARL11 expression was correlated with 17 out of 21 immune infiltrates, including CD8+ T cells and M2 macrophages, described as having anti-tumoral activity. Likewise, ARL11 is interconnected with ZAP70, ADAM17, and P2RX7, which are implicated in immune cell activation. Collectively, this study provides the first evidence that ARL1, ARL11, and ARL15 may influence CM progression, prognosis, and immune microenvironment remodeling.
BGP-15 Inhibits Hyperglycemia-Aggravated VSMC Calcification Induced by High Phosphate
Authors: Nagy Annamária , Pethő Dávid , Gesztelyi Rudolf , Juhász Béla , Balla György , Szilvássy Zoltán , Balla József , Gáll Tamás
Published: August 2021

Vascular calcification associated with high plasma phosphate (Pi) level is a frequent complication of hyperglycemia, diabetes mellitus, and chronic kidney disease. BGP-15 is an emerging anti-diabetic drug candidate. This study was aimed to explore whether BGP-15 inhibits high Pi-induced calcification of human vascular smooth muscle cells (VSMCs) under normal glucose (NG) and high glucose (HG) conditions. Exposure of VSMCs to Pi resulted in accumulation of extracellular calcium, elevated cellular Pi uptake and intracellular pyruvate dehydrogenase kinase-4 (PDK-4) level, loss of smooth muscle cell markers (ACTA, TAGLN), and enhanced osteochondrogenic gene expression (KLF-5, Msx-2, Sp7, BMP-2). Increased Annexin A2 and decreased matrix Gla protein (MGP) content were found in extracellular vesicles (EVs). The HG condition markedly aggravated Pi-induced VSMC calcification. BGP-15 inhibited Pi uptake and PDK-4 expression that was accompanied by the decreased nuclear translocation of KLF-5, Msx-2, Sp7, retained VSMC markers (ACTA, TAGLN), and decreased BMP-2 in both NG and HG conditions. EVs exhibited increased MGP content and decreased Annexin A2. Importantly, BGP-15 prevented the deposition of calcium in the extracellular matrix. In conclusion, BGP-15 inhibits Pi-induced osteochondrogenic phenotypic switch and mineralization of VSMCs in vitro that make BGP-15 an ideal candidate to attenuate both diabetic and non-diabetic vascular calcification.
Development of a Method of Measuring β-D-Glucan and Its Use in Preemptive Therapy for Invasive Fungal Infections
Authors: Yoshida Minoru
Published: August 2021

Invasive fungal infections (IFIs) are serious infections that develop in conjunction with neutropenia after chemotherapy for acute leukemia or with hematopoietic stem cell transplantation. Conventionally, empirical antifungal therapy was recommended to treat IFIs for patient safety despite a lack of evidence of fungal infections. However, many studies have indicated that antifungals were not necessary for over half of patients, and several detriments of empirical therapy were noted, e.g., antifungals caused adverse reactions, an increase in drug-resistant fungi was a possibility, and medical costs soared. β-D-glucan (BDG) is a component of clinically important fungi such as Aspergillus and Candida. The G-test was developed in Japan as a way to measure BDG in serum using a coagulation factor from the blood of the horseshoe crab. Pre-emptive antifungal therapy based upon serodiagnosis with a BDG or galactomannan assay and CT imaging has been introduced. With pre-emptive antifungal therapy, the prognosis is equivalent to that with empirical therapy, and the dose of the antifungal has been successfully reduced. Measurement of BDG has been adopted widely as a method of diagnosing IFIs and is listed in the key guidelines for fungal infections and febrile neutropenia.
Dermal Olfactory Receptor OR51B5 Is Essential for Survival and Collagen Synthesis in Human Dermal Fibroblast (Hs68 Cells)
Authors: Son Bomin , Kang Wesuk , Park Soyoon , Choi Dabin , Park Taesun
Published: August 2021

Skin dermis comprises extracellular matrix components, mainly collagen fibers. A decrease in collagen synthesis caused by several factors, including ultraviolet (UV) irradiation and stress, eventually causes extrinsic skin aging. Olfactory receptors (ORs) were initially considered to be specifically expressed in nasal tissue, but several ORs have been reported to be present in other tissues, and their biological roles have recently received increasing attention. In this study, we aimed to characterize the role of ORs in cell survival and collagen synthesis in dermal fibroblasts. We confirmed that UVB irradiation and dexamethasone exposure significantly decreased cell survival and collagen synthesis in Hs68 dermal fibroblasts. Moreover, we demonstrated that the mRNA expression of 10 ORs detectable in Hs68 cells was significantly downregulated in aged conditions compared with that in normal conditions. Thereafter, by individual knockdown of the 10 candidate ORs, we identified that only OR51B5 knockdown leads to a reduction of cell survival and collagen synthesis. OR51B5 knockdown decreased cAMP levels and dampened the downstream protein kinase A/cAMP-response element binding protein pathway, downregulating the survival- and collagen synthesis-related genes in the dermal fibroblasts. Therefore, OR51B5 may be an interesting candidate that plays a role in cell survival and collagen synthesis.
The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast Yarrowia lipolytica
Authors: Hapeta Piotr , Szczepańska Patrycja , Witkowski Tadeusz , Nicaud Jean-Marc , Crutz-Le Coq Anne-Marie , Lazar Zbigniew
Published: August 2021

The development of efficient bioprocesses requires inexpensive and renewable substrates. Molasses, a by-product of the sugar industry, contains mostly sucrose, a disaccharide composed of glucose and fructose, both easily absorbed by microorganisms. Yarrowia lipolytica, a platform for the production of various chemicals, can be engineered for sucrose utilization by heterologous invertase expression, yet the problem of preferential use of glucose over fructose remains, as fructose consumption begins only after glucose depletion what significantly extends the bioprocesses. We investigated the role of hexose transporters and hexokinase (native and fructophilic) in this preference. Analysis of growth profiles and kinetics of monosaccharide utilization has proven that the glucose preference in Y. lipolytica depends primarily on the affinity of native hexokinase for glucose. Interestingly, combined overexpression of either hexokinase with hexose transporters significantly accelerated citric acid biosynthesis and enhanced pentose phosphate pathway leading to secretion of polyols (31.5 g/L vs. no polyols in the control strain). So far, polyol biosynthesis was efficient in glycerol-containing media. Moreover, overexpression of fructophilic hexokinase in combination with hexose transporters not only shortened this process to 48 h (84 h for the medium with glycerol) but also allowed to obtain 23% more polyols (40 g/L) compared to the glycerol medium (32.5 g/L).
Enhanced Performance of Zn/Br Flow Battery Using N-Methyl-N-Propylmorpholinium Bromide as Complexing Agent
Authors: Jiménez-Blasco Uxua , Moreno Eduardo , Cólera Maura , Díaz-Carrasco Pilar , Arrebola José C. , Caballero Alvaro , Morales Julián , Vargas Óscar A.
Published: August 2021

Redox flow batteries (RFB) are one of the most interesting technologies in the field of energy storage, since they allow the decoupling of power and capacity. Zinc–bromine flow batteries (ZBFB) are a type of hybrid RFB, as the capacity depends on the effective area of the negative electrode (anode), on which metallic zinc is deposited during the charging process. Gaseous bromine is generated at the positive electrode (cathode) during the charging process, so the use of bromine complexing agents (BCA) is very important. These BCAs are quaternary amines capable of complexation with bromine and generating an organic phase, immiscible with the aqueous electrolyte. One of the most commonly used BCAs in RFB technology is 4-methylethylmorpholinium bromide (MEM-Br). In this work, an alternative quaternary amine 4-methylpropylmorpholinium bromide (MPM-Br) was studied. MPM-Br was integrated into the electrolyte, and 200 charge–discharge cycles were performed on the resulting ZBFBs. The obtained results were compared with those when MEM-Br was used, and it was observed that the electrolyte with MPM-Br displays a higher resistance in voltage and higher energy efficiency, making it a promising alternative to MEM-Br.
High-Throughput NanoBiT-Based Screening for Inhibitors of HIV-1 Vpu and Host BST-2 Protein Interaction
Authors: Li Boye , Dong Xiaoxiao , Zhang Wenmei , Chen Tian , Yu Boyang , Zhao Wenyue , Yang Yishu , Wang Xiaoli , Hu Qin , Wang Xiayan
Published: August 2021

Bone marrow stromal cell antigen 2 (BST-2), also known as CD317 or tetherin, has been identified as a host restriction factor that suppresses the release of enveloped viruses from host cells by physically tethering viral particles to the cell surface; however, this host defense can be subverted by multiple viruses. For example, human immunodeficiency virus (HIV)-1 encodes a specific accessory protein, viral protein U (Vpu), to counteract BST-2 by binding to it and directing its lysosomal degradation. Thus, blocking the interaction between Vpu and BST-2 will provide a promising strategy for anti-HIV therapy. Here, we report a NanoLuc Binary Technology (NanoBiT)-based high-throughput screening assay to detect inhibitors that disrupt the Vpu-BST-2 interaction. Out of more than 1000 compounds screened, four inhibitors were identified with strong activity at nontoxic concentrations. In subsequent cell-based BST-2 degradation assays, inhibitor Y-39983 HCl restored the cell-surface and total cellular level of BST-2 in the presence of Vpu. Furthermore, the Vpu-mediated enhancement of pesudotyped viral particle production was inhibited by Y-39983 HCl. Our findings indicate that our newly developed assay can be used for the discovery of potential antiviral molecules with novel mechanisms of action.
Starve to Sustain—An Ancient Syrian Landrace of Sorghum as Tool for Phosphorous Bio-Economy?
Authors: Kanbar Adnan , Mirzai Madeleine , Abuslima Eman , Flubacher Noemi , Eghbalian Rose , Garbev Krassimir , Bergfeldt Britta , Ullrich Angela , Leibold Hans , Eiche Elisabeth , Müller Mario , Mokry Markus , Stapf Dieter , Nick Peter
Published: August 2021

Phosphorus (P) is an essential macronutrient, playing a role in developmental and metabolic processes in plants. To understand the local and systemic responses of sorghum to inorganic phosphorus (Pi) starvation and the potential of straw and ash for reutilisation in agriculture, we compared two grain (Razinieh) and sweet (Della) sorghum varieties with respect to their morpho-physiological and molecular responses. We found that Pi starvation increased the elongation of primary roots, the formation of lateral roots, and the accumulation of anthocyanin. In Razinieh, lateral roots were promoted to a higher extent, correlated with a higher expression of SbPht1 phosphate transporters. Infrared spectra of straw from mature plants raised to maturity showed two prominent bands at 1371 and 2337 cm−1, which could be assigned to P-H(H2) stretching vibration in phosphine acid and phosphinothious acid, and their derivates, whose abundance correlated with phosphate uptake of the source plant and genotype (with a higher intensity in Razinieh). The ash generated from these straws stimulated the shoot elongation and root development of the rice seedlings, especially for the material derived from Razinieh raised under Pi starvation. In conclusion, sorghum growing on marginal lands has potential as a bio-economy alternative for mineral phosphorus recycling.
Obérisk: Cybersecurity Requirements Elicitation through Agile Remote or Face-to-Face Risk Management Brainstorming Sessions
Authors: Paul Stéphane , Naouar Douraid , Gureghian Emmanuel
Published: August 2021

Cyberattacks make the news daily. Systems must be appropriately secured. Cybersecurity risk analyses are more than ever necessary, but… traveling and gathering in a room to discuss the topic has become difficult due to the COVID, whilst having a cybersecurity expert working isolated with an electronic support tool is clearly not the solution. In this article, we describe and illustrate Obérisk, an agile, cross-disciplinary and Obeya-like approach to risk management that equally supports face-to-face or remote risk management brainstorming sessions. The approach has matured for the last three years by using it for training and a wide range of real industrial projects. The overall approach is detailed and illustrated on a naval use case, with extensive feedback from the end-users. We show that Obérisk is really time-efficient and effective at managing risks at the early stages of a project, whilst remaining extremely low-cost. As the project grows or when the system is deployed, it may eventually be necessary to shift to a more comprehensive commercial electronic support tool.
Study of DNA Interaction and Cytotoxicity Activity of Oxidovanadium(V) Complexes with ONO Donor Schiff Base Ligands
Authors: Sahu Gurunath , Tiekink Edward R. T. , Dinda Rupam
Published: August 2021

Two new oxidovanadium(V) complexes, (HNEt3)[VVO2L] (1) and [(VVOL)2μ-O] (2), have been synthesized using a tridentate Schiff base ligand H2L [where H2L = 4-((E)-(2-hydroxy-5-nitrophenylimino)methyl)benzene-1,3-diol] and VO(acac)2 as starting metal precursor. The ligand and corresponding metal complexes are characterized by physicochemical (elemental analysis), spectroscopic (FT-IR, UV–Vis, and NMR), and spectrometric (ESI–MS) methods. X-ray crystallographic analysis indicates the anion in salt 1 features a distorted square-pyramidal geometry for the vanadium(V) center defined by imine-N, two phenoxide-O, and two oxido-O atoms. The interaction of the compounds with CT–DNA was studied through UV–Vis absorption titration and circular dichroism methods. The results indicated that complexes showed enhanced binding affinity towards DNA compared to the ligand molecule. Finally, the in vitro cytotoxicity studies of H2L, 1, and 2 were evaluated against colon cancer (HT-29) and mouse embryonic fibroblast (NIH-3T3) cell lines by MTT assay. The results demonstrated that the compounds manifested a cytotoxic potential comparable with clinically referred drugs and caused cell death by apoptosis.
Harnessing the Therapeutic Potential of Decoys in Non-Atherosclerotic Cardiovascular Diseases: State of the Art
Authors: Mahjoubin-Tehran Maryam , Atkin Stephen L. , Bezsonov Evgeny E. , Jamialahmadi Tannaz , Sahebkar Amirhossein
Published: August 2021

Cardiovascular disease (CVD) is the main cause of global death, highlighting the fact that conventional therapeutic approaches for the treatment of CVD patients are insufficient, and there is a need to develop new therapeutic approaches. In recent years, decoy technology, decoy oligodeoxynucleotides (ODN), and decoy peptides show promising results for the future treatment of CVDs. Decoy ODN inhibits transcription by binding to the transcriptional factor, while decoy peptide neutralizes receptors by binding to the ligands. This review focused on studies that have investigated the effects of decoy ODN and decoy peptides on non-atherosclerotic CVD.
Improving the Alignment of Dynamic Sheet-Formed Mats by Changing Nozzle Geometry and Their Reinforcement of Polypropylene Matrix Composites
Authors: Sunny Tom , Pickering Kim L. , McDonald-Wharry John
Published: August 2021

The main objective of this study was to improve the orientation of fibres within the mats produced using dynamic sheet forming (DSF). DSF is used to make fibre mats by forcing a fibre suspension through a nozzle onto a rotating drum. In this research, the effect of nozzle geometry on the orientation of hemp fibres within DSF mats was investigated. The orientation of fibres within the mats produced was assessed using ImageJ (OrientationJ) and X-ray diffraction. It was found that, as the contraction ratio of the nozzle increased, the orientation of fibres within the fibre mats increased. It was also found that the composite tensile strength increased with increased fibre orientation.
Comparative Study of Data Matrix Codes Localization and Recognition Methods
Authors: Karrach Ladislav , Pivarčiová Elena
Published: August 2021

We provide a comprehensive and in-depth overview of the various approaches applicable to the recognition of Data Matrix codes in arbitrary images. All presented methods use the typical “L” shaped Finder Pattern to locate the Data Matrix code in the image. Well-known image processing techniques such as edge detection, adaptive thresholding, or connected component labeling are used to identify the Finder Pattern. The recognition rate of the compared methods was tested on a set of images with Data Matrix codes, which is published together with the article. The experimental results show that methods based on adaptive thresholding achieved a better recognition rate than methods based on edge detection.