bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeting GGT1 Eliminates the Tumor-Promoting Effect and Enhanced Immunosuppressive Function of Myeloid-Derived Suppressor Cells Caused by G-CSF
Authors: Xie Zhiqi , Kawasaki Takahiro , Zhou Haoyang , Okuzaki Daisuke , Okada Naoki , Tachibana Masashi
Published: April 2022

Myeloid-derived suppressor cells (MDSCs) are major immunosuppressive cells that accumulate in tumor-bearing hosts. Since MDSCs suppress anti-tumor immunity and promote tumor progression, they are promising targets for cancer immunotherapy. Granulocyte colony-stimulating factor (G-CSF) is an agent used for the treatment of chemotherapy-induced febrile neutropenia (FN) in patients with cancer. However, several reports have revealed that G-CSF plays crucial immune-related adverse roles in tumor progression through MDSCs. In this study, we showed that MDSCs differentiated in the presence of G-CSF in vitro exhibited enhanced proliferation and immunosuppressive activity compared to those differentiated without G-CSF. RNA sequencing analysis demonstrated that G-CSF enhanced the immunosuppressive function of MDSCs by upregulating gamma-glutamyltransferase (GGT) 1. Moreover, in the EL4 lymphoma-bearing neutropenic mouse model, administration of recombinant G-CSF increased the number of MDSCs and attenuated the anti-cancer effect of chemotherapy. We showed that the combination of GGsTop, a GGT inhibitor, could prevent G-CSF-induced tumor growth, without affecting the promotion of myelopoiesis by G-CSF. These results suggest that targeting GGT1 can mitigate G-CSF-induced enhanced immunosuppressive functions of MDSCs and can eliminate the tumor-promoting effect of G-CSF. Furthermore, GGsTop could be an attractive combination agent during G-CSF treatment for FN in patients with cancer.
Deciphering the Underlying Mechanisms of Formula Le-Cao-Shi Against Liver Injuries by Integrating Network Pharmacology, Metabonomics, and Experimental Validation
Authors: Zhao Qing , Ren Xia , Song Shu-Yue , Yu Ri-Lei , Li Xin , Zhang Peng , Shao Chang-Lun , Wang Chang-Yun
Published: April 2022

Le-Cao-Shi (LCS) has long been used as a folk traditional Chinese medicine formula against liver injuries, whereas its pharmacological mechanisms remain elusive. Our study aims to investigate the underlying mechanism of LCS in treating liver injuries via integrated network pharmacology, metabonomics, and experimental validation. By network pharmacology, 57 compounds were screened as candidate compounds based on ADME parameters from the LCS compound bank (213 compounds collected from the literature of three single herbs). According to online compound–target databases, the aforementioned candidate compounds were predicted to target 87 potential targets related to liver injuries. More than 15 pathways connected with these potential targets were considered vital pathways in collectively modulating liver injuries, which were found to be relevant to cancer, xenobiotic metabolism by cytochrome P450 enzymes, bile secretion, inflammation, and antioxidation. Metabonomics analysis by using the supernatant of the rat liver homogenate with UPLC-Q-TOF/MS demonstrated that 18 potential biomarkers could be regulated by LCS, which was closely related to linoleic acid metabolism, glutathione metabolism, cysteine and methionine metabolism, and glycerophospholipid metabolism pathways. Linoleic acid metabolism and glutathione metabolism pathways were two key common pathways in both network pharmacology and metabonomics analysis. In ELISA experiments with the CCl4-induced rat liver injury model, LCS was found to significantly reduce the levels of inflammatory parameters, decrease liver malondialdehyde (MDA) levels, and enhance the activities of hepatic antioxidant enzymes, which validated that LCS could inhibit liver injuries through anti-inflammatory property and by suppressing lipid peroxidation and improving the antioxidant defense system. Our work could provide new insights into the underlying pharmacological mechanisms of LCS against liver injuries, which is beneficial for its further investigation and modernization.
Extra Virgin Olive Oil-Based Green Formulations With Promising Antimicrobial Activity Against Drug-Resistant Isolates
Authors: Di Pietro Marisa , Filardo Simone , Mattioli Roberto , Francioso Antonio , Raponi Giammarco , Mosca Luciana , Sessa Rosa
Published: April 2022

Extra virgin olive oil (EVOO) from Olea europaea L. drupes, a cornerstone in the Mediterranean diet, is well known for its nutritional and health properties, especially for prevention of cardiovascular diseases and metabolic disorders. Traditionally, beneficial health effects have been largely attributed to the high concentration of monounsaturated fatty acids, and in recent years, these have also been related to other components including oleacein and oleocanthal. Here, we evaluated, for the first time, the antimicrobial activity of different green extra virgin olive oil-based formulations in natural deep eutectic solvents (NaDESs) emerging as powerful and biocompatible solvents. Specifically, the antimicrobial activity of the EVOO extract, as well as purified oleocanthal and oleacein in two NaDESs (choline/glycerol and choline/propylene glycol), against several drug-resistant clinical isolates and standard microbial strains has been evaluated. The main result was the inhibitory activity of the EVOO extract in choline/glycerol as well as oleacein in choline/propylene glycol toward drug-resistant Gram-positive and -negative strains. Specifically, the EVOO extract in choline/glycerol showed the highest antibacterial activity against several clinical strains of Staphylococcus aureus, whereas oleacein in choline/propylene glycol was the most effective toward various clinical strains of Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. In addition, all the formulations tested were effective against Candida spp. In conclusion, our results suggest EVOO-based formulations in NaDESs as an interesting strategy that may help in reducing the risk of development of drug resistance. Under this perspective, the usage of NaDESs for the preparation of new antimicrobial formulations may represent a promising approach.
β-Glucan Combined With PD-1/PD-L1 Checkpoint Blockade for Immunotherapy in Patients With Advanced Cancer
Authors: Wang Mengjie , Bai Yu , Pei Jiaxin , Li Dongqing , Pu Xiaolin , Zhu Wenyu , Xia Lei , Qi Chunjian , Jiang Hua , Ning Yongling
Published: April 2022

Programmed death-1 (PD-1)/PD-ligand 1 (PD-L1) checkpoint blocking antibodies have been shown to be a powerful immune checkpoint blockade (ICB) therapy for patients with cancer. However, patients quickly develop resistance to immunotherapy. β-glucan, an immune adjuvant, has been found to stimulate innate and adaptive immune responses. In this study, we assessed the use of whole glucan particle (WGP) β-glucan in combination with PD-1/PD-L1–blocking antibodies to slow down the resistance to immunotherapy. Results from a tumor mouse model demonstrated that administration of WGP β-glucan plus PD-1/PD-L1–blocking antibodies led to increased recruitment of immune-associated cells, improved regulation of the balance between T-cell activation and immune tolerance, and delayed tumor progression. This combination therapy was also found to improve progression-free survival in patients with advanced cancer who had previously discontinued anti-PD-1/PD-L1 because of disease progression. These findings suggest that β-glucan could be used as an immune adjuvant to reverse anti-PD-1/PD-L1 resistance by regulating the immune system.
Scalar Field and Particle Dynamics in Conformal Frame
Authors: Arbab Arbab I. , Albughylil Razan S. , Alfedeel Alnadhief H. A.
Published: April 2022

The dynamics of the scalar field and particle in a conformal frame are considered. The conformal Klein-Gordon equation describing the scalar field is transformed into the quantum Telegraph equation in Minkowski space-time. The conformal factor acts like a background field having a perfect energy-momentum tensor. The scalar field decays exponentially with time during inflation allowing the conformal field to induce space energy. The conformal field grows with time at the expense of decreasing the energy density of the real scalar field. Einstein’s tensor embodies an energy-momentum tensor representing the background contribution reflecting the matter aspect of the gravitational field. The energy density arising from the conformal field is negative. The background energy associated with Einstein’s curvature tensor gives rise to massive gravitons that act like a cosmological constant. In an expanding Universe, this particular case yields a background energy proportional to the square of the scalar field mass giving rise to the massive graviton. Because of the background fluid, which is intrinsically coupled to space curvature, the particle’s motion is found to exhibit a drag force and therefore moves in a curved path even no matter around exists. It is found that breaking the conformal invariance gives rise to the mass generation of gravitons.

PACS 04.20.-q, Classical general relativity; PACS 04.20.Cv, Fundamental problems and general formalism; PACS 95.30.Sf, Relativity and gravitation; PACS 4.62.+v, Quantum fields in curved space-time
Influence of an Acute Exposure to a Moderate Real Altitude on Motoneuron Pool Excitability and Jumping Performance
Authors: Štirn Igor , Garcia-Ramos Amador , Feriche Belen , Strojnik Vojko , Tomažin Katja
Published: April 2022

The aim of the study was to test whether ascending to a moderate real altitude affects motoneuron pool excitability at rest, as expressed by a change in the H-reflex amplitude, and also to elucidate whether a possible alteration in the motoneuron pool excitability could be reflected in the execution of lower-body concentric explosive (squat jump; SJ) and fast eccentric-concentric (drop jump; DJ) muscle actions. Fifteen participants performed four experimental sessions that consisted of the combination of two real altitude conditions [low altitude (low altitude, 690 m), high altitude (higher altitude, 2,320 m)] and two testing procedures (H-reflex and vertical jumps). Participants were tested on each testing day at 8, 11, 14 and 17 h. The only significant difference (p < 0.05) detected for the H-reflex was the higher H-reflex response (25.6%) obtained 15 min after arrival at altitude compared to baseline measurement. In terms of motor behavior, DJ height was the only variable that showed a significant interaction between altitude conditions (LA and HA) and time of measurement (8, 11, 14 and 17 h) as DJ height increased more during successive measurements at HA compared to LA. The only significant difference between the LA and HA conditions was observed for DJ height at 17 h which was higher for the HA condition (p = 0.04, ES = 0.41). Although an increased H-reflex response was detected after a brief (15–20 min) exposure to real altitude, the effect on motorneuron pool excitability could not be confirmed since no significant changes in the H-reflex were detected when comparing LA and HA. On the other hand, the positive effect of altitude on DJ performance was accentuated after 6 h of exposure.
Refining Our Understanding of the Flow Through Coronary Artery Branches; Revisiting Murray’s Law in Human Epicardial Coronary Arteries
Authors: Taylor Daniel J. , Feher Jeroen , Halliday Ian , Hose D. Rodney , Gosling Rebecca , Aubiniere-Robb Louise , van ‘t Veer Marcel , Keulards Danielle , Tonino Pim A. L. , Rochette Michel , Gunn Julian , Morris Paul D.
Published: April 2022

Background: Quantification of coronary blood flow is used to evaluate coronary artery disease, but our understanding of flow through branched systems is poor. Murray’s law defines coronary morphometric scaling, the relationship between flow (Q) and vessel diameter (D) and is the basis for minimum lumen area targets when intervening on bifurcation lesions. Murray’s original law (Q α DP) dictates that the exponent (P) is 3.0, whilst constant blood velocity throughout the system would suggest an exponent of 2.0. In human coronary arteries, the value of Murray’s exponent remains unknown.

Aim: To establish the exponent in Murray’s power law relationship that best reproduces coronary blood flows (Q) and microvascular resistances (Rmicro) in a bifurcating coronary tree.

Methods and Results: We screened 48 cases, and were able to evaluate inlet Q and Rmicro in 27 branched coronary arteries, taken from 20 patients, using a novel computational fluid dynamics (CFD) model which reconstructs 3D coronary anatomy from angiography and uses pressure-wire measurements to compute Q and Rmicro distribution in the main- and side-branches. Outputs were validated against invasive measurements using a Rayflow™ catheter. A Murray’s power law exponent of 2.15 produced the strongest correlation and closest agreement with inlet Q (zero bias, r = 0.47, p = 0.006) and an exponent of 2.38 produced the strongest correlation and closest agreement with Rmicro (zero bias, r = 0.66, p = 0.0001).

Conclusions: The optimal power law exponents for Q and Rmicro were not 3.0, as dictated by Murray’s Law, but 2.15 and 2.38 respectively. These data will be useful in assessing patient-specific coronary physiology and tailoring revascularisation decisions.
Roles of Endomembrane Alkali Cation/Proton Exchangers in Synaptic Function and Neurodevelopmental Disorders
Authors: Gao Andy Y. L. , Lourdin-De Filippis Etienne , Orlowski John , McKinney R. Anne
Published: April 2022

Endomembrane alkali cation (Na+, K+)/proton (H+) exchangers (eNHEs) are increasingly associated with neurological disorders. These eNHEs play integral roles in regulating the luminal pH, processing, and trafficking of cargo along the secretory (Golgi and post-Golgi vesicles) and endocytic (early, recycling, and late endosomes) pathways, essential regulatory processes vital for neuronal development and plasticity. Given the complex morphology and compartmentalization of multipolar neurons, the contribution of eNHEs in maintaining optimal pH homeostasis and cargo trafficking is especially significant during periods of structural and functional development and remodeling. While the importance of eNHEs has been demonstrated in a variety of non-neuronal cell types, their involvement in neuronal function is less well understood. In this review, we will discuss their emerging roles in excitatory synaptic function, particularly as it pertains to cellular learning and remodeling. We will also explore their connections to neurodevelopmental conditions, including intellectual disability, autism, and attention deficit hyperactivity disorders.
Time to Train: The Involvement of the Molecular Clock in Exercise Adaptation of Skeletal Muscle
Authors: Mansingh Shivani , Handschin Christoph
Published: April 2022

Circadian rhythms regulate a host of physiological processes in a time-dependent manner to maintain homeostasis in response to various environmental stimuli like day and night cycles, food intake, and physical activity. Disruptions in circadian rhythms due to genetic mutations, shift work, exposure to artificial light sources, aberrant eating habits, and abnormal sleep cycles can have dire consequences for health. Importantly, exercise training efficiently ameliorates many of these adverse effects and the role of skeletal muscle in mediating the benefits of exercise is a topic of great interest. However, the molecular and physiological interactions between the clock, skeletal muscle function and exercise are poorly understood, and are most likely a combination of molecular clock components directly acting in muscle as well as in concordance with other peripheral metabolic organ systems like the liver. This review aims to consolidate existing experimental evidence on the involvement of molecular clock factors in exercise adaptation of skeletal muscle and to highlight the existing gaps in knowledge that need to be investigated to develop therapeutic avenues for diseases that are associated with these systems.
Combining Rice Straw Biochar With Leguminous Cover Crop as Green Manure and Mineral Fertilizer Enhances Soil Microbial Biomass and Rice Yield in South China
Authors: Xie Zhijian , Shah Farooq , Zhou Chunhuo
Published: April 2022

Whether combining rice-straw biochar (RSB) with leguminous cover crop (LCC) has synergistic effects in the rice production system or not, is still unknown. Two pot experiments were conducted to systematically explore the impacts of RSB on mass decomposition and nitrogen (N) release from LCC residues after incorporation into acidic paddy soil. Similarly, the effect of combining these two factors on soil nutrient status and microbial biomasses in the rice production system was also examined. Five treatments, namely, no N fertilizer (CK), 100% N fertilizer (150 kg N ha–1 as N100), 80% N fertilizer plus RSB (N80B), LCC (N80M), and a combination of RSB with LCC (N80BM), were included. The results indicated that biomass decomposition and N release pattern followed a double exponential decay model such that the addition of RSB slightly stimulated the rates of both mass decomposition and N release during the initial rapid phase of decomposition. Thereafter, it notably slowed down the rates of both these parameters during the relatively slower stage of incorporating LCC residues to paddy soil during early rice season. Compared to 100% N, applying 80% N in conjunction with RSB and/or LCC residue increased grain yield and its components (i.e., effective panicles, 1,000-grain weight, and fully filled grains) that subsequently increased N accumulation and its physiological use efficiency (PUEN) of rice shoot. Moreover, under 20% N, applying RSB and/or LCC residue remarkably increased the soil organic matter and total N, and soil microbial populations and biomasses, while the contents of NH4+ and NO3– were decreased in RSB-amended paddy soil (N80B and N80BM), in comparison with N100. Thus, combining RSB with LCC residue is a novel and promising management intervention for reducing mineral fertilizer use, improving soil fertility and rice production, and consequently minimizing the overall production cost in south China.
Determinants of Genetic Structure in a Highly Heterogeneous Landscape in Southwest China
Authors: Wambulwa Moses C. , Luo Ya-Huang , Zhu Guang-Fu , Milne Richard , Wachira Francis N. , Wu Zeng-Yuan , Wang Hong , Gao Lian-Ming , Li De-Zhu , Liu Jie
Published: April 2022

Intra-specific genetic diversity is a fundamental component of biodiversity, and is key to species adaptation and persistence. However, significant knowledge gaps still exist in our understanding of the patterns of genetic diversity and their key determinants. Most previous investigations mainly utilized single-species and/or a limited number of explanatory variables; so here we mapped the patterns of plastid genetic diversity within 15 plant species, and explored the key determinants shaping these patterns using a wide range of variables. Population-level cpDNA sequence data for 15 plant species from the Longitudinal Range Gorge Region (LRGR), southwest China, were retrieved from literature and used to estimate haplotype diversity (HD) and population pairwise genetic differentiation (FST) indices. Genetic diversity and divergence landscape surfaces were then generated based on theHDandFST, respectively, to clarify the patterns of genetic structure in the region. Subsequently, we analyzed the relationships between plastid genetic diversity and 16 explanatory variables (classified as anthropogenic, climatic, and topographic). We found that the highest genetic diversity occurred in the Yulong Mountain region, with a significant proportion (~74.81%) of the high diversity land area being located outside of protected areas. The highest genetic divergence was observed approximately along the 25°N latitudinal line, with notable peaks in the western and eastern edges of the LRGR. Genetic diversity (HD) was weakly but significantly positively correlated with both Latitude (lat) and Annual Mean Wet Day Frequency (wet), yet significantly negatively correlated with all of Longitude (long), Annual Mean Cloud Cover Percent (cld), Annual Mean Anthropogenic Flux (ahf), and Human Footprint Index (hfp). A combination of climatic, topographic, and anthropogenic factors explained a significant proportion (78%) of genetic variation, with topographic factors (latandlong) being the best predictors. Our analysis identified areas of high genetic diversity (genetic diversity “hotspots”) and divergence in the region, and these should be prioritized for conservation. This study contributes to a better understanding of the features that shape the distribution of plastid genetic diversity in the LRGR and thus would inform conservation management efforts in this species-rich, but vulnerable region.
Variability of Flowering Sex and Its Effect on Agronomic Trait Expression in White Guinea Yam
Authors: Iseki Kohtaro , Matsumoto Ryo , Olaleye Olajumoke , Shimizu Motoki , Asfaw Asrat
Published: April 2022

White Guinea yam (Dioscorea rotundata) is mainly a dioecious tuberous crop that produces flowers of varying sex phenotypes. Agronomic traits in Guinea yam differ according to the sex phenotype, but the precise interaction between the traits and sex phenotype is not clearly understood. This might be due to the high heterozygosity of yam where cultivars with different flowering sex have different genetic backgrounds, which mask the sole effect of sex phenotype on the agronomic traits. This study used F1-derived clonal progenies from a bi-parental cross to minimize the impact of different genetic backgrounds among the plants with different sex phenotypes. The impact of plant sex on agronomic traits, specifically tuber yield, was evaluated through field trials conducted for four years. The results showed that only plants with a female genotype exhibited varying sex phenotypes even within the clones of same accession grown in the same experimental field. The significant effects of sex genotype and phenotype on agronomic traits were detected. Our results revealed that the flowering date was delayed in the plants with female genotypes compared to male genotypes, even when compared only among the plants with male phenotypes. The flowering date is the most important reason for the sexual differences in tuber yield. A high tuber yield was obtained when plants with the female phenotype flowered before tuber enlargement. This result can be attributed to the fact that the low flowering intensity in female plants increases the availability of carbon resources for leaf development. Female plants also showed a large negative effect of late flowering on tuber yield owing to resource competition between flowering and tuber enlargement. These findings demonstrate the feasibility of yield improvement by controlling the flowering time, with a higher effectiveness achieved in female than in male plants.
Parallel Evolution of C-Type Lectin Domain Gene Family Sizes in Insect-Vectored Nematodes
Authors: Ning Jing , Zhou Jiao , Wang Haixiang , Liu Yaning , Ahmad Faheem , Feng Xiaohui , Fu Yu , Gu Xiaoting , Zhao Lilin
Published: April 2022

The dispersal stage of pathogens is crucial for the successful spread and infection of their hosts. Some plant-parasitic nematodes (PPNs) have evolved specialized dispersal stages to reach healthy hosts by being carried out by insect vectors. Because gene gain and loss is a major factor contributing to the evolution of novel characteristics, it is essential to clarify the gene family characteristics among nematodes with different dispersal modes to disentangle the evolution of insect-mediated dispersal. Here, the size of the C-type lectin (CTL) family genes of insect-vectored nematodes was found to be drastically reduced compared with those of self-dispersing nematodes, whereas the diversity of their functional domains was significantly higher. The gene family sizes of vector-dispersed nematodes were only a twentieth of the size of that of a self-dispersing (i.e., without a biotic vector) nematode model Caenorhabditis elegans, and these genes were inactive during the dispersal stage. Phylogenetic analysis showed that some CTL genes of vector-borne PPNs shared higher homology to the animal parasitic nematodes compared with other PPNs. Moreover, homology modeling predicted that the CTLs of insect-vectored nematodes bear remarkable structural similarity to the lectin genes of their vector's immune system. Because CTL genes are important sugar-binding proteins for the innate immune response of C. elegans, the loss of some CTL genes of vector-transmitted PPNs might be responsible for their parallel adaptations to a mutualistic relationship with their vector. These results expand our understanding of the evolutionary benefits of vector-mediated transmission for the nematode and vector-nematode co-evolution.
Whole Genome Resequencing of 20 Accessions of Rice Landraces Reveals Javanica Genomic Structure Variation and Allelic Genotypes of a Grain Weight Gene TGW2
Authors: Long Weixiong , Luo Lihua , Luo Laiyang , Xu Weibiao , Li Yonghui , Cai Yaohui , Xie Hongwei
Published: April 2022

The landraces preserved by indigenous worldwide exhibited larger variation in the phenotypes and adaption to different environments, which suggests that they comprise rich resources and can be served as a gene pool for rice improvement. Despite extensive studies on cultivated rice, the variations and relationships between landraces and modern cultivated rice remain unclear. In this study, a total of 20 varieties that include 10 Oryza javanica collected from different countries worldwide and 10 Oryza indica from China were genotyped and yielded a sum of 99.9-Gb resequencing raw data. With the genomic sequence of the japonica cultivar Nipponbare as a reference, the following genetic features of single-nucleotide polymorphism (SNP) ranged from 861,177 to 1,044,617, insertion–deletion polymorphisms (InDels) ranged from 164,018 to 211,135, and structural variation (SV) ranged from 3,313 to 4,959 were identified in Oryza javanica. Variation between the two subspecies was also determined that 584,104 SNPs, 75,351 InDels, 104,606 SNPs, and 19,872 InDels specific to Oryza indica and Oryza javanica, respectively. Furthermore, Gene Ontology (GO) and KEGG of Oryza javanica-specific SNP-related genes revealed that they participated in DNA metabolic process, DNA replication, and DNA integration. The sequence variation and candidate grain shape-related gene TGW2 were identified through Fst and sweep selective analysis. Hap4 of TGW2 is performed better than others. The whole genome sequence data and genetic variation information illustrated in this study will serve as an important gene pool for molecular breeding and facilitate genetic analysis of Oryza javanica varieties.
AtRsmD Is Required for Chloroplast Development and Chloroplast Function in Arabidopsis thaliana
Authors: Wang Zi-Yuan , Qu Wan-Tong , Mei Tong , Zhang Nan , Yang Nai-Ying , Xu Xiao-Feng , Xiong Hai-Bo , Yang Zhong-Nan , Yu Qing-Bo
Published: April 2022

AtRsmD was recently demonstrated to be a chloroplast 16S rRNA methyltransferase (MTase) for the m2G915 modification in Arabidopsis. Here, its function of AtRsmD for chloroplast development and photosynthesis was further analyzed. The AtRsmD gene is highly expressed in green photosynthetic tissues. AtRsmD is associated with the thylakoid in chloroplasts. The atrsmd-2 mutant exhibited impaired photosynthetic efficiency in emerging leaves under normal growth conditions. A few thylakoid lamellas could be observed in the chloroplast from the atrsmd-2 mutant, and these thylakoids were loosely organized. Knockout of the AtRsmD gene had minor effects on chloroplast ribosome biogenesis and RNA loading on chloroplast ribosomes, but it reduced the amounts of chloroplast-encoded photosynthesis-related proteins in the emerging leaves, for example, D1, D2, CP43, and CP47, which reduced the accumulation of the photosynthetic complex. Nevertheless, knockout of the AtRsmD gene did not cause a general reduction in chloroplast-encoded proteins in Arabidopsis grown under normal growth conditions. Additionally, the atrsmd-2 mutant exhibited more sensitivity to lincomycin, which specifically inhibits the elongation of nascent polypeptide chains. Cold stress exacerbated the effect on chloroplast ribosome biogenesis in the atrsmd-2 mutant. All these data suggest that the AtRsmD protein plays distinct regulatory roles in chloroplast translation, which is required for chloroplast development and chloroplast function.
Contrasting Effects of Nitrogen Addition on Vegetative Phenology in Dry and Wet Years in a Temperate Steppe on the Mongolian Plateau
Authors: Zhou Zhenxing , Zhang Liwei , Liu Yinzhan , Zhang Kunpeng , Wang Wenrui , Zhu Junkang , Chai Shijie , Zhang Huiying , Miao Yuan
Published: April 2022

Changes in spring and autumn phenology and thus growing season length (GSL) pose great challenges in accurately predicting terrestrial primary productivity. However, how spring and autumn phenology in response to land-use change and nitrogen deposition and underlying mechanisms remain unclear. This study was conducted to explore the GSL and its components [i.e., the beginning of growing season and ending of growing season (EGS)] in response to mowing and nitrogen addition in a temperate steppe on the Mongolia Plateau during 2 years with hydrologically contrasting condition [dry (2014) vs. wet (2015)]. Our results demonstrated that mowing advanced the BGS only by 3.83 days, while nitrogen addition advanced and delayed the BGS and EGS by 2.85 and 3.31 days, respectively, and thus prolonged the GSL by 6.16 days across the two growing seasons from 2014 to 2015. When analyzed by each year, nitrogen addition lengthened the GSL in the dry year (2014), whereas it shortened the GSL in the wet year (2015). Further analyses revealed that the contrasting impacts of nitrogen on the GSL were attributed to monthly precipitation regimes and plant growth rate indicated by the maximum of normalized difference vegetation index (NDVmax). Moreover, changes in the GSL and its two components had divergent impacts on community productivity. The findings highlight the critical role of precipitation regimes in regulating the responses of spring and autumn phenology to nutrient enrichment and suggest that the relationships of ecosystem productivity with spring and autumn phenology largely depend on interannual precipitation fluctuations under future increased nitrogen deposition scenarios.
Monitoring Autophagy in Rice With GFP-ATG8 Marker Lines
Authors: Liu Rui , Zhang Rongxue , Yang Yi , Liu Xuejun , Gong Qingqiu
Published: April 2022

Autophagy is a conserved intracellular trafficking pathway for bulk degradation and recycling of cellular components in eukaryotes. The hallmark of autophagy is the formation of double-membraned vesicles termed autophagosomes, which selectively or non-selectively pack up various macromolecules and organelles and deliver these cargoes into the vacuole/lysosome. Like all other membrane trafficking pathways, the observation of autophagy is largely dependent on marker lines. ATG8/LC3 is the only autophagy-related (ATG) protein that, through a covalent bond to phosphatidylethanolamine (PE), associates tightly with the isolation membrane/pre-autophagosomal structure (PAS), the growing phagophore, the mature autophagosome, and the autophagic bodies. Therefore, fluorescent protein (FP)-tagged ATG8 had been widely used for monitoring autophagosome formation and autophagic flux. In rice (Oryza sativa), FP-OsATG8 driven by Cauliflower mosaic virus (CaMV) 35S promoter had been used for imaging autophagosome and autophagic bodies. Here, we constructed three vectors carrying GFP-OsATG8a, driven by 35S, ubiquitin, and the endogenous ATG8a promoter, individually. Then, we compared them for their suitability in monitoring autophagy, by observing GFP-ATG8a puncta formation in transiently transformed rice protoplasts, and by tracking the autophagic flux with GFP-ATG8 cleavage assay in rice stable transgenic lines. GFP-Trap immunoprecipitation and mass spectrometry were also performed with the three marker lines to show that they can be used reliably for proteomic studies. We found out that the ubiquitin promoter is the best for protoplast imaging. Transgenic rice seedlings of the three marker lines showed comparable performance in autophagic flux measurement using the GFP-ATG8 cleavage assay. Surprisingly, the levels of GFP-ATG8a transcripts and protein contents were similar in all marker lines, indicating post-transcriptional regulation of the transgene expression by a yet unknown mechanism. These marker lines can serve as useful tools for autophagy studies in rice.
Optical Property Mapping of Apples and the Relationship With Quality Properties
Authors: Peng Hehuan , Zhang Chang , Sun Zhizhong , Sun Tong , Hu Dong , Yang Zidong , Wang Jinshuang
Published: April 2022

This paper reports on the measurement of optical property mapping of apples at the wavelengths of 460, 527, 630, and 710 nm using spatial-frequency domain imaging (SFDI) technique, for assessing the soluble solid content (SSC), firmness, and color parameters. A laboratory-based multispectral SFDI system was developed for acquiring SFDI of 140 “Golden Delicious” apples, from which absorption coefficient (μa) and reduced scattering coefficient (μs′) mappings were quantitatively determined using the three-phase demodulation coupled with curve-fitting method. There was no noticeable spatial variation in the optical property mapping based on the resulting effect of different sizes of the region of interest (ROI) on the average optical properties. Support vector machine (SVM), multiple linear regression (MLR), and partial least square (PLS) models were developed based on μa, μs′ and their combinations (μa × μs′ and μeff) for predicting apple qualities, among which SVM outperformed the best. Better prediction results for quality parameters based on the μa were observed than those based on the μs′, and the combinations further improved the prediction performance, compared to the individual μa or μs′. The best prediction models for SSC and firmness parameters [slope, flesh firmness (FF), and maximum force (Max.F)] were achieved based on the μa × μs′, whereas those for color parameters of b* and C* were based on the μeff, with the correlation coefficients of prediction as 0.66, 0.68, 0.73, 0.79, 0.86, and 0.86, respectively.
Comprehensive Evolutionary Analysis of CPP Genes in Brassica napus L. and Its Two Diploid Progenitors Revealing the Potential Molecular Basis of Allopolyploid Adaptive Advantage Under Salt Stress
Authors: Li Mengdi , Wang Fan , Ma Jiayu , Liu Hengzhao , Ye Hang , Zhao Peng , Wang Jianbo
Published: April 2022

Allopolyploids exist widely in nature and have strong environmental adaptability. The typical allopolyploid Brassica napus L. is a widely cultivated crop, but whether it is superior to its diploid progenitors in abiotic stress resistance and the key genes that may be involved are not fully understood. Cystein-rich polycomb-like protein (CPP) genes encode critical transcription factors involved in the response of abiotic stress, including salt stress. To explore the potential molecular basis of allopolyploid adaptation to salt stress, we comprehensively analyzed the characteristics and salt stress response of the CPP genes in B. napus and its two diploid progenitors in this study. We found some molecular basis that might be associated with the adaptability of B. napus, including the expansion of the CPP gene family, the acquisition of introns by some BnCPPs, and abundant cis-acting elements upstream of BnCPPs. We found two duplication modes (whole genome duplication and transposed duplication) might be the main reasons for the expansion of CPP gene family in B. napus during allopolyploidization. CPP gene expression levels and several physiological indexes were changed in B. napus and its diploid progenitors after salt stress, suggesting that CPP genes might play important roles in the response of salt stress. We found that some BnCPPs might undergo new functionalization or subfunctionalization, and some BnCPPs also show biased expression, which might contribute to the adaptation of B. napus under saline environment. Compared with diploid progenitors, B. napus showed stronger physiological responses, and BnCPP gene expression also showed higher changes after salt stress, indicating that the allopolyploid B. napus had an adaptive advantage under salt stress. This study could provide evidence for the adaptability of polyploid and provide important clues for the study of the molecular mechanism of salt stress resistance in B. napus.
iTRAQ Proteomic Analysis of Wheat (Triticum aestivum L.) Genotypes Differing in Waterlogging Tolerance
Authors: Yang Rui , Li Murong , Harrison Matthew Tom , Fahad Shah , Wei Mingmei , Li Xiu , Yin Lijun , Sha Aihua , Zhou Meixue , Liu Ke , Wang Xiaoyan
Published: April 2022

Transient and chronic waterlogging constrains crop production in many regions of the world. Here, we invoke a novel iTRAQ-based proteomic strategy to elicit protein synthesis and regulation responses to waterlogging in tolerant (XM 55) and sensitive genotypes (YM 158). Of the 7,710 proteins identified, 16 were distinct between the two genotypes under waterlogging, partially defining a proteomic basis for waterlogging tolerance (and sensitivity). We found that 11 proteins were up-regulated and 5 proteins were down-regulated; the former included an Fe-S cluster assembly factor, heat shock cognate 70, GTP-binding protein SAR1A-like and CBS domain-containing protein. Down-regulated proteins contained photosystem II reaction center protein H, carotenoid 9, 10 (9′, 10′)-cleavage dioxygenase-like, psbP-like protein 1 and mitochondrial ATPase inhibitor. We showed that nine proteins responded to waterlogging with non-cultivar specificity: these included 3-isopropylmalate dehydratase large subunit, solanesyl-diphosphate synthase 2, DEAD-box ATP-dependent RNA helicase 3, and 3 predicted or uncharacterized proteins. Sixteen of the 28 selected proteins showed consistent expression patterns between mRNA and protein levels. We conclude that waterlogging stress may redirect protein synthesis, reduce chlorophyll synthesis and enzyme abundance involved in photorespiration, thus influencing synthesis of other metabolic enzymes. Collectively, these factors accelerate the accumulation of harmful metabolites in leaves in waterlogging-susceptible genotypes. The differentially expressed proteins enumerated here could be used as biological markers for enhancing waterlogging tolerance as part of future crop breeding programs.
Recent Developments in Deciphering the Biological Role of Plant Complex N-Glycans
Authors: Strasser Richard
Published: April 2022

Asparagine (N)-linked protein glycosylation is a ubiquitous co- and posttranslational modification which has a huge impact on the biogenesis and function of proteins and consequently on the development, growth, and physiology of organisms. In mammals, N-glycan processing carried out by Golgi-resident glycosidases and glycosyltransferases creates a number of structurally diverse N-glycans with specific roles in many different biological processes. In plants, complex N-glycan modifications like the attachment of β1,2-xylose, core α1,3-fucose, or the Lewis A-type structures are evolutionary highly conserved, but their biological function is poorly known. Here, I highlight recent developments that contribute to a better understanding of these conserved glycoprotein modifications and discuss future directions to move the field forward.
Measuring Theory of Mind in Adolescents With Language and Communication Problems: An Ecological Perspective
Authors: Smit Lidy , Knoors Harry , Rabeling-Keus Inge , Verhoeven Ludo , Vissers Constance
Published: April 2022

We tested if the newly designed ToMotion task reflects a single construct and if the atypical groups differ in their performance compared to typically developing peers. Furthermore, we were interested if ToMotion maps a developmental sequence in a Theory of Mind (ToM) performance as exemplified by increasing difficulty of the questions asked in every item. The sample consisted of 13 adolescents that have been diagnosed with a developmental language disorder (DLD) and 14 adolescents that are deaf or hard of hearing (DHH). All of these adolescents were in special schools for secondary vocational education. The control group existed of 34 typical developing adolescents (TD) who were in regular intermediate vocational education, ranging from level 2 to 4. The ToMotion, available in a spoken Dutch version and in a version in Sign Language of the Netherlands (SLN), was used to map ToM abilities. An attempt has been made to fill the gap of missing studies of ToM in adolescents by developing a new measuring instrument. In conclusion, assessing ToM with the ToMotion results in a picture that DHH adolescents score lower than TD peers. However, their scores are as consistent as those of the TD peers. The picture of DLD adolescents is the reverse. They show no differences in ToM scores, but seem to be somewhat more inconsistent compared to TD peers. We provide a discussion on those results and its implications for future research. What this paper adds? The current study introduces a new visual Theory of Mind (ToM) task, ToMotion, designed specifically to assess ToM in adolescents in an ecologically valid way and adapted to the needs of adolescents with language and communication difficulties.
Validation of Polish Version of Dispositional Flow Scale-2 and Flow State Scale-2 Questionnaires
Authors: Józefowicz Justyna , Kowalczyk-Grębska Natalia , Brzezicka Aneta
Published: April 2022

The aim of this study is to evaluate the psychometric properties of the Polish version of the Dispositional Flow Scale-2 (DFS-2) and Flow State Scale-2 (FSS-2), for use with Polish adults and young adults. Currently, there are no tools that would allow us to study flow among Polish speakers. At the same time, due to the great interest in flow and its potential importance for effectiveness, cooperation, and learning, it is worth ensuring that reliable validated measurement questionnaires are available for people studying the Polish population. Study participants completed 856 questionnaires, of which 496 individuals (with an average age of 36.31 years) participated in the DFS-2 study and 360 individuals (with an average age of 33.46 years) participated in the FSS-2 study. The maximum likelihood estimator (MLR) was selected for the CFA analysis. Model fit was assessed using: χ2, comparative fit index (CFI), Tucker-Lewis index (TLI), and standardized root mean square of residuals (SRMR), and root mean square of approximation error (RMSEA). For both questionnaires, formative first-level models with nine factors and second-level models with nine factors loaded on a higher-order flow factor were compared using the Satorra-Bentler Scaled difference χ2 test. The ω coefficient was used to estimate the reliability of the FFS-2 and DFS-2 models tested in the CFA method. Confirmatory factor analysis of both DFS-2 structural models showed satisfactory model fit. Most of the fit indices for the hierarchical 2nd order FSS-2 model presented satisfactory values, except for SRMR. Both DFS-2 and FSS-2 factors tested in the analysis showed good reliability (ω ≥ 0.7). Our findings confirmed the reliability and validity of the Polish versions of DFS-2 and FSS-2 scales. The scales are reliable when applied to Polish adults and young adults.
Sense of Coherence as a Mediator in the Association Between Empathy and Moods in Healthcare Professionals: The Moderating Effect of Age
Authors: Hori Miyo , Yoshikawa Eisho , Hayama Daichi , Sakamoto Shigeko , Okada Tsuneo , Sakai Yoshinori , Fujiwara Hideomi , Takayanagi Kazue , Murakami Kazuo , Ohnishi Junji
Published: April 2022

While empathy is considered a critical determinant of the quality of medical care, growing evidence suggests it may be associated with both one’s own positive and negative moods among healthcare professionals. Meanwhile, sense of coherence (SOC) plays an essential role in the improvement of both psychological and physical health. Reportedly, individual SOC reaches full stability after around age 30. The aim of this study was first to evaluate the mediatory role of SOC on the association between empathy and individual moods among 114 healthcare professionals in a general hospital, and then to examine the moderating effect of age on this association. Participants completed a range of self-report demographic questionnaires, Empathy Process Scale (EPS), the 13-item Antonovsky’s SOC, and Profile of Mood States (POMS). Findings showed that SOC mediated the relations between empathy (EPS) and both POMS-Vigor (POMS-V: self-vigor mood) and POMS-Depression (POMS-D: self-depression mood). Notably, moderated mediation analysis revealed that there was a significant interaction (age × SOC) on self-vigor mood (POMS-V) in healthcare professionals. The indirect effect of empathy (EPS) on self-vigor mood (POMS-V) through SOC was significant at over mean age “32.83.” Although there was no significant interaction with age regarding the indirect effect of empathy (EPS) on self-depression mood (POMS-D), in the sub-category level analysis of empathy (EPS), we found a significant interaction item [age × empathy for other’s negative affect (EPS-N)] on SOC. This indirect effect was also significant at over mean age “32.83.” Taken, together, the current study highlighted the significant mediator of SOC on that empathy amplifies self-vigor mood and attenuates self-depression mood as a protective factor among the Japanese healthcare professionals. Some components of these processes may depend on the moderating role of age, indicating that we may need to consider the SOC development with age for more effective empathy performance interventions among healthcare professionals.
Spanish Pre-Olympic Athletes’ Motivations and Barriers to Pursuing Dual Career as a Function of Sociodemographic, Sport and Academic Variables
Authors: Mateo-Orcajada Adrián , Leiva-Arcas Alejandro , Vaquero-Cristóbal Raquel , Abenza-Cano Lucía , García-Roca Juan Alfonso , Meroño Lourdes , Isidori Emanuele , Sánchez-Pato Antonio
Published: April 2022

The dual career allows elite athletes to attain their maximum competitive and academic performance, but the COVID-19 pandemic hindered their development and changed their perception of the importance given to the sporting and educational environment. For this reason, the aim of the present study was to determine the differences in the motivations and perceived barriers, the importance given to academic qualifications, and the perception of the dual career from a multifactorial perspective, of elite athletes according to sex, type of sport practiced, job performance, time of sports career, type of athlete, and type of scholarship received. A total of 100 student-athletes participated in the research study by completing the “Perceptions of dual career student-athletes” questionnaire. The results showed that athletes from individual modalities (p = 0.012) and those who did not receive any scholarships described more barriers (p < 0.001). In addition, women studied more because they enjoyed it (p = 0.007); athletes from individual modalities studied to work later (p = 0.008); athletes who do not work perceived a greater influence between study and sports performance (p = 0.029); at the beginning and at the best stage of their sports career, a greater influence of academics on performance was perceived (p = 0.016); and athletes who considered themselves professionals, and athletes who did not receive any scholarships (p = 0.025), reported that the conciliation between sports and academic life was difficult (p = 0.034). The results obtained point to the importance of dual career scholarships for student-athletes, as well as the need for the programs implemented for these athletes to consider sex, sport modality or type of scholarship granted.