bluuebunny/crossref_metadata_2025 huggingface dataset:
Regulating smoking in Japan: from manners to rules
Authors: Arrington Celeste L.
Published: February 2024

Opinions vary in Japan on whether smoking is deviant today, but the behavior, once widely accepted, faces increasing regulation. Recent reforms, moving beyond reliance on nonsmokers' tolerance and smokers' etiquette, impose stricter and more detailed rules on smoking, along with penalties for noncompliance. As the Japanese government's promotional materials note, the reforms move “from manners to rules” (manā kara rūru e). The evolution of Japan's smoking regulations exemplifies a shift toward more legalistic modes of social control. Historically, Japanese governance relied on non-binding “soft law,” administrative guidance, and societal cooperation. Legalistic governance, in contrast, hinges on formal rules and proceduralized enforcement mechanisms. This article, drawing on twenty-eight interviews and qualitative analysis of policy deliberations, advocacy organization documents, court rulings, and Japanese news coverage, traces how societal actors contributed to this legalistic turn. Tobacco control advocates filed lawsuits, pursued voluntary changes through local activities, and provided information subsidies to policymakers while lobbying for local and national reforms. They thereby helped de-normalize smoking and render it regulatable. By uncovering bottom-up drivers of legalistic governance and the strategies through which societal actors influence regulatory style, this paper contributes to scholarship on governance, policy diffusion, and law and social change.
Backlash after Quotas: Moral Panic as a Soft Repression Tactic against Women Politicians
Authors: Shiran Myriam
Published: February 2024

Advocates of gender quotas emphasize their transformative potential for women’s political participation. Yet evidence on the symbolic effects of quotas remains inconclusive, with some studies uncovering significant backlash after implementation. Although elite resistance to quotas has been posited as an explanation, the underlying mechanisms generating negative effects remain underexplored. This study proposes the utilization of “moral panic” by elites as a mechanism of resistance. By leveraging their media influence and employing conservative moral rhetoric, elites engineer moral panic, framing women’s political engagement as detrimental to social order and gender hierarchies. Such panic aims to incite public opposition and rationalize elite resistance to progressive changes. Notably, this tactic is more prevalent in countries with reserved-seat quotas, where elites possess limited control over electoral outcomes. Using an original dataset encompassing politician names and genders, I analyze over 150,000 news articles from 2000 to 2021 across 10 sub-Saharan African countries. The findings indicate that quota-induced gains in women’s representation are followed by significant increases in conservative ethical language in news coverage of women politicians, particularly in countries with reserved-seat quotas. These findings bear important implications for gender equality in politics and shed light on the dynamics of backlash after quota implementation.
Strengthening the medicolegal system: Fulfilling international law obligations during conflicts and disasters to prevent and resolve issues of humanitarian concern
Authors: Morcillo Mendez Maria Dolores
Published: February 2024

In times of armed conflict, disasters and violence, people may become separated from their families, go missing or die, or become victims of ill-treatment and sexual violence. Under international humanitarian law, States have obligations to prevent harm and address humanitarian needs. At State level, the medicolegal system is conventionally mandated to address these needs and fulfil related legal obligations. In practice, State responses can sometimes be non-existent, limited by the endemic functionality of existing systems, or degraded by crises of violence, natural disasters and migration. These conditions can, in turn, impede the establishment of peace, reparations, restorative justice efforts and post-conflict reconstruction. This paper explains what a medicolegal system is and the entities that encompass it. The paper highlights the importance of developing clear policies, regulation and procedures that ensure proper functioning of the system. It reviews common gaps and challenges that limit State prevention and response to issues of humanitarian concern. Finally, recommendations when developing and implementing humanitarian programmes to strengthen medicolegal systems are provided, with a particular focus on the content of protection dialogue in diplomatic fora.
Supplementation of the maternal diet with Brazil nut (Bertholletia excelsa H.B.K.) prevents cognitive impairment in the offspring of obese mothers
Authors: Apolinário Lilian Fioravanso , Silva Amanda Tais , Rosa Andrielli Pompermayer , Oliveira Cleber da Silva , Lira Cleberson , Guerra João Pedro Costa dos Santos , Friedrich Júlia Furtado , Rosa Letícia Queiroz , Chelegão Rodrigo , Botelho Sílvia de Carvalho Campos , Sinhorin Valéria Dornelles Gindri , de Oliveira Júlio Cezar , Velloso Nádia Aléssio
Published: February 2024

Maternal obesity may trigger long-term neurodevelopmental disorders in offspring. Considering the benefits of the Brazil nut (Bertholletia excelsa H.B.K.), a rich source of nutrients such as selenium, this study aimed to evaluate its effect on the behavior of obese rat offspring and its relationship with oxidative stress. From 60 days of age until weaning, female Wistar rats were fed a high-fat diet (mHF) or an HF diet supplemented with 5% Brazil nut (mHF/BN), while control mothers (mCTL) were fed a standard diet or a standard diet supplemented with 5% Brazil nut (mBN). Male pups received a standard diet throughout life and, at 30 and 90 days old, were subjected to behavioral tasks to evaluate anxiety and cognition. Biochemical evaluations were performed at 90 days of age. No alterations were observed in the anxiety behavior of the offspring. However, the offspring of the mHF group (oHF) exhibited impaired short-term memory at 30 and 90 days of age and impaired long-term memory at 30 days. Short-term memory impairment was prevented by Brazil nuts in young rats (30 days). While the serum selenium concentration was reduced in the oHF group, the serum catalase concentration was reduced in all groups, without changes in lipid peroxidation or protein carbonylation. Brazil nut maternal diet supplementation prevented short- and long-term cognitive impairment in the offspring, which may be related to the selenium levels.
Trends in Twin Births and Survival in Bangladesh: An Analysis of Half a Century of Evidence
Authors: Hossain Kazi Zubair , Hasan Iftekhar
Published: February 2024

This study assessed the trends in twin births and their survival in Bangladesh by analyzing over a quarter million live births during 1970–2018, pooled from all eight rounds of the Bangladesh Demographic and Health Survey. In these five decades, the twinning rate increased by 1.5 times, from 5.8 to 8.6 twins per 1000 maternities. The decadal twinning rates varied across maternal age, parity, body mass index, household wealth index, and geographic region. The gap in decadal neonatal, infant, and under-five cumulative survival probability between singleton and multiple births was found to be closing, using Kaplan-Meier curves. Child mortality decreased by 80% and 60% in singleton and multiple births respectively. However, the absolute size of child mortality in multiple births remained six times higher than in singletons and was concentrated in the neonatal period. The share of multiple births surged in all types of child mortality. We predict a further and faster rise in multiple births in the coming decades in the face of upward trends in maternal age overlapping with higher parities, education, career prospects, contraceptive use, and the future demand-supply of assisted reproductive technology. A particular focus on the improvement of perinatal and neonatal care with wider availability is warranted. Otherwise, increased multiple births might raise child mortality and create public health challenges.
Seasonal and Interannual Variations of Global Tides in the Mesosphere and Lower Thermosphere Neutral Winds: II. Semidiurnal and Terdiurnal Tides
Authors: Liu Yangkun , Xu Jiyao , Smith A. K. , Liu Xiao
Published: February 2024

In the mesosphere and lower thermosphere (MLT) region, semidiurnal and terdiurnal tides are dominant at middle and high latitudes and are important for the dynamics and structures. Using the global neutral horizontal wind data in the MLT region observed by TIMED Doppler interferometer from 2002 to 2021, the seasonal and interannual variations of six semidiurnal and terdiurnal tidal components (SW2, SW3, SW4, TW3, TW2 and TW4) are investigated. Particularly, the responses of these tidal components to the stratospheric quasi‐biennial oscillation (SQBO) and the solar cycle are presented. The results indicate that: (a) seasonal and interannual variations of these tidal components are pronounced in their peak regions. The peak region is defined as the region where the ratio of the annual mean amplitude to the maximum annual mean amplitude is larger than 0.8. (b) In their peak regions, migrating tidal components exhibit strong seasonal variations at annual (annual oscillations (AO)), semiannual (semiannual oscillations (SAO)) and terannual (terannual oscillations (TAO)) periods, whereas nonmigrating tidal components only exhibit prominent AO and SAO. (c) In their peak regions, the responses of the annual mean amplitudes of these tidal components to SQBO are negative; the responses of migrating tidal components to solar cycle are negative whereas those of nonmigrating tidal components are positive. (d) For these tidal components, the responses of the amplitudes of seasonal variations to SQBO and solar cycle are weaker than their annual mean amplitudes, and the latitude × altitude response patterns are not always consistent with those of the annual mean amplitudes.
Propagation of Very Oblique Chorus Waves Near a Plasmaspheric Plume Boundary
Authors: Gu Wenyao , Hartley David , Liu Xu , Chen Lunjin , Bortnik Jacob , Horne Richard B.
Published: February 2024

In a case study using Van Allen Probe B, we investigate chorus wave observations near the western edge of a plasmaspheric plume characterized by steep density gradients. Initially, wave vectors are oriented anti‐Earthward, but they become very oblique and eastward as the probe approaches the plume boundary. Treating the plume boundary as an azimuthal density gradient, ray tracing can reproduce the observed wave vector directions. Ray tracing shows that the azimuthal density gradient strongly inclines the wave vectors eastward. Consequently, waves are reflected upon reaching the Gendrin angle and cannot enter the plume. We establish an analytical criterion for the azimuthal density enhancement, determining the condition for chorus waves to enter plumes near the equatorial region. Our results partly explain the oblique chorus near plumes observed by Hartley, Chen et al. (2022, https://doi.org/10.1029/2022GL098710), offering insight into wave‐particle interactions by chorus waves with the influence of azimuthal density structures.
Superthermal Electron Observations at Mars During the December 2022 Disappearing Solar Wind Event
Authors: Xu Shaosui , Mitchell David L. , Halekas Jasper , Brain David A. , Weber Tristan , Andersson Laila , Shaver Skylar , Azari Abigail , McFadden James P. , Hanley Kathleen , Ma Yingjuan , Lee Christina , DiBraccio Gina A. , Mazelle Christian , Curry Shannon M.
Published: February 2024

On 26–27 December 2022, Mars experienced an extremely low‐density solar wind stream, which was encountered first by Earth because of the radial alignment of the two planets (i.e., Mars opposition). During this event, two important properties of the ionospheric and magnetospheric states changed significantly in response to the low solar wind ram pressure, as inferred from the superthermal electron observations from the Mars Atmospheric and Volatile EvolutioN (MAVEN) mission. The interface between the ionosphere and magnetosphere expanded to thousands of kilometers, outside of the nominal bow shock locations, coinciding with the expansion of the cold planetary ions. Meanwhile, the ambipolar electrostatic potential arising from the ionospheric electron pressure gradient increased from the nominal ∼ −0.7 to ∼ −2 V (relative to the lower ionosphere). This enhanced ambipolar potential likely facilitated the observed ionosphere expansion.
Simulating Long‐Term Dynamics of Radiation Belt Electrons Using DREAM3D Model
Authors: Lee Sang‐Yun , Tu Weichao , Cunningham Gregory S. , Cowee Misa M. , Wang Dedong , Shprits Yuri Y. , Henderson Michael G. , Reeves Geoffrey D.
Published: February 2024

We compared the performance of DREAM3D simulations in reproducing the long‐term radiation belt dynamics observed by Van Allen Probes over the entire year of 2017 with various boundary conditions (BCs) and model inputs. Specifically, we investigated the effects of three different outer boundary conditions, two different low‐energy boundary conditions for seed electrons, four different radial diffusion (RD) coefficients (DLL), four hiss wave models, and two chorus wave models from the literature. Using the outer boundary condition driven by GOES data, our benchmark simulation generally well reproduces the observed radiation belt dynamics inside L* = 6, with a better model performance at lower μ than higher μ, where μ is the first adiabatic invariant. By varying the boundary conditions and inputs, we find that: (a) The data‐driven outer boundary condition is critical to the model performance, while adding in the data‐driven seed population doesn't further improve the performance. (b) The model shows comparable performance with DLL from Brautigam and Albert (2000, https://doi.org/10.1029/1999ja900344), Ozeke et al. (2014, https://doi.org/10.1002/2013ja019204), and Liu et al. (2016, https://doi.org/10.1002/2015gl067398), while with DLL from Ali et al. (2016, https://doi.org/10.1002/2016ja023002) the model shows less RD compared to data. (c) The model performance is similar with data‐based hiss models, but the results show faster loss is still needed inside the plasmasphere. (d) The model performs similarly with the two different chorus models, but better capturing the electron enhancement at higher μ using the Wang et al. (2019, https://doi.org/10.1029/2018ja026183) model due to its stronger wave power, since local heating for higher energy electrons is under‐reproduced in the current model.
Modeling Physical and Physiological Processes Reveals the Role of Turbulence in the Prerequisites for Microcystis Blooms
Authors: Li Na , Gao Xueping , Sun Bowen
Published: February 2024

Harmful algal blooms of Microcystis have become a global problem. Turbulence, a determining factor affecting blooms, not only disperses surface scum but also controls the growth of Microcystis. Numerous studies have analyzed the effects of turbulence on the growth and colony size of Microcystis in laboratories, but the turbulence thresholds for Microcystis growth and colony disaggregation in the field are difficult to determine due to the complex environment. In addition, the quantitative contribution of turbulence‐driven blooms and the intrinsic mechanisms of the spatial distribution responding to turbulence are unclear. In this study, a fully integrated filed scale computational model focusing on turbulence‐driven blooms was developed, which incorporates physical processes (turbulence‐induced vertical mixing, VMT) and physiological processes such as buoyancy‐controlling transport (BCT), turbulence‐induced colony size variation (CSV), and growth rate variation (GRV). We performed model sensitivity analysis and evaluated the effects of turbulence intensity and duration on the biomass and vertical distribution of Microcystis. The results show that the optimal turbulence dissipation rate for Microcystis growth in the field is 1.0 × 10−5 m2/s3 and the critical turbulence dissipation rate for aggregation distribution is 3.81 × 10−6 m2/s3 in shallow lakes. A quantitative comparison of the effects of physical/physiological processes on blooms shows that physiological processes (CSV, GRV, and BCT) are critical for biomass enrichment, and the accumulation of Microcystis at the water surface is dominated by physical processes (VMT). This study reveals the mechanisms of turbulence‐driven Microcystis blooms and provides new insights for algal bloom prediction and control.
Automated Earthwork Detection Using Topological Persistence
Authors: Lapides Dana A. , Grindstaff Gillian , Nichols Mary H.
Published: February 2024

For thousands of years, humans have altered the movement of water through construction of earthworks. These earthworks remain in landscapes, where they continue to alter hydrology, even where structures have long since been abandoned. Management of lands containing earthworks requires an understanding of how the earthworks impact hydrology and knowledge of where the structures are located in the landscape. Various methods for detecting topographic features exist in the literature, including a set of rule and threshold‐based techniques and machine learning methods. These tools are either labor‐intensive or require special pre‐processing or a priori assumptions about structures that limit generalizability. Here, we test a topological analysis tool called “persistence” to determine if it is useful for earthwork detection in rangelands. We found that persistence can be used to detect earthworks with 83% precision and 64% accuracy. Breached berms and berms with significant upslope sedimentation are most likely not to be detected using persistence. These results indicate that persistence can be useful for terrain analysis, and it has the potential to substantially reduce manual effort in feature detection by identifying regions where berms may be found.
Effect of parental adverse childhood experiences on intergenerational DNA methylation signatures from peripheral blood mononuclear cells and buccal mucosa
Authors: Mohazzab-Hosseinian Sahra , Garcia Erika , Wiemels Joseph , Marconett Crystal , Corona Karina , Howe Caitlin G. , Foley Helen , Farzan Shohreh F. , Bastain Theresa M. , Breton Carrie V.
Published: February 2024

In this study, the effect of cumulative ACEs experienced on human maternal DNA methylation (DNAm) was estimated while accounting for interaction with domains of ACEs in prenatal peripheral blood mononuclear cell samples from the Maternal and Developmental Risks from Environmental Stressors (MADRES) pregnancy cohort. The intergenerational transmission of ACE-associated DNAm was also explored used paired maternal (N = 120) and neonatal cord blood (N = 69) samples. Replication in buccal samples was explored in the Children’s Health Study (CHS) among adult parental (N = 31) and pediatric (N = 114) samples. We used a four-level categorical indicator variable for ACEs exposure: none (0 ACEs), low (1–3 ACEs), moderate (4–6 ACEs), and high (>6 ACEs). Effects of ACEs on maternal DNAm (N = 240) were estimated using linear models. To evaluate evidence for intergenerational transmission, mediation analysis (N = 60 mother-child pairs) was used. Analysis of maternal samples displayed some shared but mostly distinct effects of ACEs on DNAm across low, moderate, and high ACEs categories. CLCN7 and PTPRN2 was associated with maternal DNAm in the low ACE group and this association replicated in the CHS. CLCN7 was also nominally significant in the gene expression correlation analysis among maternal profiles (N = 35), along with 11 other genes. ACE-associated methylation was observed in maternal and neonatal profiles in the COMT promoter region, with some evidence of mediation by maternal COMT methylation. Specific genomic loci exhibited mutually exclusive maternal ACE effects on DNAm in either maternal or neonatal population. There is some evidence for an intergenerational effect of ACEs, supported by shared DNAm signatures in the COMT gene across maternal-neonatal paired samples.
Personalized functional network mapping for autism spectrum disorder and attention-deficit/hyperactivity disorder
Authors: Zhang Jiang , Zhang Zhiwei , Sun Hui , Ma Yingzi , Yang Jia , Chen Kexuan , Yu Xiaohui , Qin Tianwei , Zhao Tianyu , Zhang Jingyue , Chu Congying , Wang Jiaojian
Published: February 2024

Autism spectrum disorder (ASD) and Attention-deficit/hyperactivity disorder (ADHD) are two typical neurodevelopmental disorders that have a long-term impact on physical and mental health. ASD is usually comorbid with ADHD and thus shares highly overlapping clinical symptoms. Delineating the shared and distinct neurophysiological profiles is important to uncover the neurobiological mechanisms to guide better therapy. In this study, we aimed to establish the behaviors, functional connectome, and network properties differences between ASD, ADHD-Combined, and ADHD-Inattentive using resting-state functional magnetic resonance imaging. We used the non-negative matrix fraction method to define personalized large-scale functional networks for each participant. The individual large-scale functional network connectivity (FNC) and graph-theory-based complex network analyses were executed and identified shared and disorder-specific differences in FNCs and network attributes. In addition, edge-wise functional connectivity analysis revealed abnormal edge co-fluctuation amplitude and number of transitions among different groups. Taken together, our study revealed disorder-specific and -shared regional and edge-wise functional connectivity and network differences for ASD and ADHD using an individual-level functional network mapping approach, which provides new evidence for the brain functional abnormalities in ASD and ADHD and facilitates understanding the neurobiological basis for both disorders.
Low dose post-transplant cyclophosphamide and sirolimus induce mixed chimerism with CTLA4-Ig or lymphocyte depletion in an MHC-mismatched murine allotransplantation model
Authors: Kabore Mariama D. , McElrath Corbin C. , Ali Mohamed A. E. , Almengo Katherine , Gangaplara Arunakumar , Fisher Cameron , Barreto Mauricio A. , Shaikh Ahmad , Olkhanud Purevdorj B. , Xu Xin , Gaskin Deanna , Lopez-Ocasio Maria , Saxena Ankit , McCoy J. Philip , Fitzhugh Courtney D.
Published: February 2024

Allogeneic hematopoietic cell transplantation (allo-HCT) offers a curative option for patients with certain non-malignant hematological diseases. High-dose post-transplant cyclophosphamide (PT-Cy) (200 mg/kg) and sirolimus (3 mg/kg), (HiC) synergistically induce stable mixed chimerism. Further, sirolimus and cytotoxic T lymphocyte-associated antigen-4 immunoglobulin (CTLA4-Ig), also known as Abatacept (Aba), promote immune tolerance and allograft survival. Here, in a major histocompatibility complex (MHC)-mismatched allo-HCT murine model, we combined Aba and/or T-cell depleting anti-Thy1.2 (Thy) with a lower dose of PT-Cy (50 mg/kg) and Sirolimus (3 mg/kg), (LoC). While mice in the LoC group showed graft rejection, the addition of Thy to LoC induced similar donor chimerism levels when compared to the HiC group. However, the addition of Aba to LoC led to graft acceptance only in younger mice. When Thy was added to the LoC+Aba setting, graft acceptance was restored in both age groups. Engrafted groups displayed significantly reduced frequencies of recipient-specific interferon-γ-producing T cells as well as an increased frequency in regulatory T cells (Tregs) except in the LoC+Aba group. Splenocytes from engrafted mice showed no proliferation upon restimulation with Balb/c stimulators. Collectively, in combination with Aba or Thy, LoC may be considered to reduce graft rejection in patients who undergo allo-HCT.
Single-cell RNA sequencing of cervical exfoliated cells reveals potential biomarkers and cellular pathogenesis in cervical carcinogenesis
Authors: Sheng Bo , Pan Shuya , Ye Miaomiao , Liu Hejing , Zhang Jiamin , Zhao Bo , Ji Huihui , Zhu Xueqiong
Published: February 2024

Cervical cancer (CC) is a common gynecological malignancy. Despite the current screening methods have been proved effectively and significantly decreased CC morbidity and mortality, deficiencies still exist. Single-cell RNA sequencing (scRNA-seq) approach can identify the complex and rare cell populations at single-cell resolution. By scRNA-seq, the heterogeneity of tumor microenvironment across cervical carcinogenesis has been mapped and described. Whether these alterations could be detected and applied to CC screening is unclear. Herein, we performed scRNA-seq of 56,173 cervical exfoliated cells from 15 samples, including normal cervix, low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and malignancy. The present study delineated the alteration of immune and epithelial cells derived during the cervical lesion progression. A subset of lipid-associated macrophage was identified as a tumor-promoting element and could serve as a biomarker for predicting the progression of LSIL into HSIL, which was then verified by immunofluorescence. Furthermore, cell–cell communication analysis indicated the SPP1-CD44 axis might exhibit a protumor interaction between epithelial cell and macrophage. In this study, we investigated the cervical multicellular ecosystem in cervical carcinogenesis and identified potential biomarkers for early detection.
Aggregability of the SQSTM1/p62-based aggresome-like induced structures determines the sensitivity to parthanatos
Authors: Hamano Shuhei , Noguchi Takuya , Asai Yukino , Ito Ryo , Komatsu Ryuto , Sato Tetsu , Inoue Aya , Maruyama Tomoe , Kudo Tada-aki , Hirata Yusuke , Shindo Sawako , Uchida Yasuo , Hwang Gi-Wook , Matsuzawa Atsushi
Published: February 2024

Overactivation of poly (ADP-ribose) polymerase-1 (PARP-1) triggers a noncanonical form of programmed cell death (PCD) called parthanatos, yet the mechanisms of its induction are not fully understood. We have recently demonstrated that the aggresome-like induced structures (ALIS) composed of the autophagy receptor SQSTM1/p62 and K48-linked polyubiquitinated proteins (p62-based ALIS) mediate parthanatos. In this study, we identified the D1 dopamine receptor agonist YM435 as a unique parthanatos inhibitor that acts as the disaggregating agent for the p62-based ALIS. We found that YM435 structurally reduces aggregability of the ALIS, and then increases its hydrophilicity and liquidity, which prevents parthanatos. Moreover, dopamine and L-DOPA, a dopamine precursor, also prevented parthanatos by reducing the aggregability of the ALIS. Together, these observations suggest that aggregability of the p62-based ALIS determines the sensitivity to parthanatos, and the pharmacological properties of YM435 that reduces the aggregability may be suitable for therapeutic drugs for parthanatos-related diseases such as neurodegenerative diseases.
Applying valency-based immuno-selection to generate broadly cross-reactive antibodies against influenza hemagglutinins
Authors: Hinke Daniëla Maria , Anderson Ane Marie , Katta Kirankumar , Laursen Marlene Fyrstenberg , Tesfaye Demo Yemane , Werninghaus Ina Charlotta , Angeletti Davide , Grødeland Gunnveig , Bogen Bjarne , Braathen Ranveig
Published: February 2024

Conserved epitopes shared between virus subtypes are often subdominant, making it difficult to induce broadly reactive antibodies by immunization. Here, we generate a plasmid DNA mix vaccine that encodes protein heterodimers with sixteen different influenza A virus hemagglutinins (HA) representing all HA subtypes except H1 (group 1) and H7 (group 2). Each single heterodimer expresses two different HA subtypes and is targeted to MHC class II on antigen presenting cells (APC). Female mice immunized with the plasmid mix produce antibodies not only against the 16 HA subtypes, but also against non-included H1 and H7. We demonstrate that individual antibody molecules cross-react between different HAs. Furthermore, the mix vaccine induces T cell responses to conserved HA epitopes. Immunized mice are partially protected against H1 viruses. The results show that application of valency-based immuno-selection to diversified antigens can be used to direct antibody responses towards conserved (subdominant) epitopes on viral antigens.
Rat superior colliculus encodes the transition between static and dynamic vision modes
Authors: Gil Rita , Valente Mafalda , Shemesh Noam
Published: February 2024

The visual continuity illusion involves a shift in visual perception from static to dynamic vision modes when the stimuli arrive at high temporal frequency, and is critical for recognizing objects moving in the environment. However, how this illusion is encoded across the visual pathway remains poorly understood, with disparate frequency thresholds at retinal, cortical, and behavioural levels suggesting the involvement of other brain areas. Here, we employ a multimodal approach encompassing behaviour, whole-brain functional MRI, and electrophysiological measurements, for investigating the encoding of the continuity illusion in rats. Behavioural experiments report a frequency threshold of 18±2 Hz. Functional MRI reveal that superior colliculus signals transition from positive to negative at the behaviourally-driven threshold, unlike thalamic and cortical areas. Electrophysiological recordings indicate that these transitions are underpinned by neural activation/suppression. Lesions in the primary visual cortex reveal this effect to be intrinsic to the superior colliculus (under a cortical gain effect). Our findings highlight the superior colliculus’ crucial involvement in encoding temporal frequency shifts, especially the change from static to dynamic vision modes.
Income determines the impact of cash transfers on HIV/AIDS: cohort study of 22.7 million Brazilians
Authors: Silva Andréa F. , Dourado Inês , Lua Iracema , Jesus Gabriela S. , Guimarães Nathalia S. , Morais Gabriel A. S. , Anderle Rodrigo V. R. , Pescarini Julia M. , Machado Daiane B. , Santos Carlos A. S. T. , Ichihara Maria Y. , Barreto Mauricio L. , Magno Laio , Souza Luis E. , Macinko James , Rasella Davide
Published: February 2024

Living with extremely low-income is an important risk factor for HIV/AIDS and can be mitigated by conditional cash transfers. Using a cohort of 22.7 million low-income individuals during 9 years, we evaluated the effects of the world’s largest conditional cash transfer, the Programa Bolsa Família, on HIV/AIDS-related outcomes. Exposure to Programa Bolsa Família was associated with reduced AIDS incidence by 41% (RR:0.59; 95%CI:0.57-0.61), mortality by 39% (RR:0.61; 95%CI:0.57-0.64), and case fatality rates by 25% (RR:0.75; 95%CI:0.66-0.85) in the cohort, and Programa Bolsa Família effects were considerably stronger among individuals of extremely low-income [reduction of 55% for incidence (RR:0.45, 95% CI:0.42-0.47), 54% mortality (RR:0.46, 95% CI:0.42-0.49), and 37% case-fatality (RR:0.63, 95% CI:0.51 −0.76)], decreasing gradually until having no effect in individuals with higher incomes. Similar effects were observed on HIV notification. Programa Bolsa Família impact was also stronger among women and adolescents. Several sensitivity and triangulation analyses demonstrated the robustness of the results. Conditional cash transfers can significantly reduce AIDS morbidity and mortality in extremely vulnerable populations and should be considered an essential intervention to achieve AIDS-related sustainable development goals by 2030.
Perfect circular polarization of elastic waves in solid media
Authors: Lee Jeseung , Kweun Minwoo “Joshua” , Lee Woorim , Seung Hong Min , Kim Yoon Young
Published: February 2024

Elastic waves involving mechanical particle motions of solid media can couple volumetric and shear deformations, making their manipulation more difficult than electromagnetic waves. Thereby, circularly polarized waves in the elastic regime have been little explored, unlike their counterparts in the electromagnetic regime, where their practical usage has been evidenced in various applications. Here, we explore generating perfect circular polarization of elastic waves in an isotropic solid medium. We devise a novel strategy for converting a linearly polarized wave into a circularly polarized wave by employing an anisotropic medium, which induces a so-far-unexplored coupled resonance phenomenon; it describes the simultaneous occurrence of the Fabry-Pérot resonance in one diagonal plane and the quarter-wave resonance in another diagonal plane orthogonal to the former with an exact 90° out-of-phase relation. We establish a theory explaining the involved physics and validate it numerically and experimentally. As a potential application of elastic circular polarization, we present simulation results demonstrating that a circularly polarized elastic wave can detect an arbitrarily oriented crack undetectable by a linearly polarized elastic wave.
A common allele increases endometrial Wnt4 expression, with antagonistic implications for pregnancy, reproductive cancers, and endometriosis
Authors: Pavličev Mihaela , McDonough-Goldstein Caitlin E. , Zupan Andreja Moset , Muglia Lisa , Hu Yueh-Chiang , Kong Fansheng , Monangi Nagendra , Dagdas Gülay , Zupančič Nina , Maziarz Jamie , Sinner Debora , Zhang Ge , Wagner Günter , Muglia Louis
Published: February 2024

The common human SNP rs3820282 is associated with multiple phenotypes including gestational length and likelihood of endometriosis and cancer, presenting a paradigmatic pleiotropic variant. Deleterious pleiotropic mutations cause the co-occurrence of disorders either within individuals, or across population. When adverse and advantageous effects are combined, pleiotropy can maintain high population frequencies of deleterious alleles. To reveal the causal molecular mechanisms of this pleiotropic SNP, we introduced this substitution into the mouse genome by CRISPR/Cas 9. Previous work showed that rs3820282 introduces a high-affinity estrogen receptor alpha-binding site at the Wnt4 locus. Here, we show that this mutation upregulates Wnt4 transcription in endometrial stroma, following the preovulatory estrogen peak. Effects on uterine transcription include downregulation of epithelial proliferation and induction of progesterone-regulated pro-implantation genes. We propose that these changes increase uterine permissiveness to embryo invasion, whereas they decrease resistance to invasion by cancer and endometriotic foci in other estrogen-responsive tissues.
Ultrastrong and ductile steel welds achieved by fine interlocking microstructures with film-like retained austenite
Authors: Moon Joonoh , Bae Gyuyeol , Jeong Bo-Young , Shin Chansun , Kwon Min-Ji , Kim Dong-Ik , Choi Dong-Jun , Lee Bong Ho , Lee Chang-Hoon , Hong Hyun-Uk , Suh Dong-Woo , Ponge Dirk
Published: February 2024

The degradation of mechanical properties caused by grain coarsening or the formation of brittle phases during welding reduces the longevity of products. Here, we report advances in the weld quality of ultra-high strength steels by utilizing Nb and Cr instead of Ni. Sole addition of Cr, as an alternative to Ni, has limitations in developing fine weld microstructure, while it is revealed that the coupling effects of Nb and Cr additions make a finer interlocking weld microstructures with a higher fraction of retained austenite due to the decrease in austenite to acicular ferrite and bainite transformation temperature and carbon activity. As a result, an alloying design with Nb and Cr creates ultrastrong and ductile steel welds with enhanced tensile properties, impact toughness, and fatigue strength, at 45% lower material costs and lower environmental impact by removing Ni.
Structurally robust lithium-rich layered oxides for high-energy and long-lasting cathodes
Authors: Jang Ho-Young , Eum Donggun , Cho Jiung , Lim Jun , Lee Yeji , Song Jun-Hyuk , Park Hyeokjun , Kim Byunghoon , Kim Do-Hoon , Cho Sung-Pyo , Jo Sugeun , Heo Jae Hoon , Lee Sunyoung , Lim Jongwoo , Kang Kisuk
Published: February 2024

O2-type lithium-rich layered oxides, known for mitigating irreversible transition metal migration and voltage decay, provide suitable framework for exploring the inherent properties of oxygen redox. Here, we present a series of O2-type lithium-rich layered oxides exhibiting minimal structural disordering and stable voltage retention even with high anionic redox participation based on the nominal composition. Notably, we observe a distinct asymmetric lattice breathing phenomenon within the layered framework driven by excessive oxygen redox, which includes substantial particle-level mechanical stress and the microcracks formation during cycling. This chemo-mechanical degradation can be effectively mitigated by balancing the anionic and cationic redox capabilities, securing both high discharge voltage (~ 3.43 V vs. Li/Li+) and capacity (~ 200 mAh g−1) over extended cycles. The observed correlation between the oxygen redox capability and the structural evolution of the layered framework suggests the distinct intrinsic capacity fading mechanism that differs from the previously proposed voltage fading mode.
Residual ANTXR1+ myofibroblasts after chemotherapy inhibit anti-tumor immunity via YAP1 signaling pathway
Authors: Licaj Monika , Mhaidly Rana , Kieffer Yann , Croizer Hugo , Bonneau Claire , Meng Arnaud , Djerroudi Lounes , Mujangi-Ebeka Kevin , Hocine Hocine R. , Bourachot Brigitte , Magagna Ilaria , Leclere Renaud , Guyonnet Lea , Bohec Mylene , Guérin Coralie , Baulande Sylvain , Kamal Maud , Le Tourneau Christophe , Lecuru Fabrice , Becette Véronique , Rouzier Roman , Vincent-Salomon Anne , Gentric Geraldine , Mechta-Grigoriou Fatima
Published: February 2024

Although cancer-associated fibroblast (CAF) heterogeneity is well-established, the impact of chemotherapy on CAF populations remains poorly understood. Here we address this question in high-grade serous ovarian cancer (HGSOC), in which we previously identified 4 CAF populations. While the global content in stroma increases in HGSOC after chemotherapy, the proportion of FAP+ CAF (also called CAF-S1) decreases. Still, maintenance of high residual CAF-S1 content after chemotherapy is associated with reduced CD8+ T lymphocyte density and poor patient prognosis, emphasizing the importance of CAF-S1 reduction upon treatment. Single cell analysis, spatial transcriptomics and immunohistochemistry reveal that the content in the ECM-producing ANTXR1+ CAF-S1 cluster (ECM-myCAF) is the most affected by chemotherapy. Moreover, functional assays demonstrate that ECM-myCAF isolated from HGSOC reduce CD8+ T-cell cytotoxicity through a Yes Associated Protein 1 (YAP1)-dependent mechanism. Thus, efficient inhibition after treatment of YAP1-signaling pathway in the ECM-myCAF cluster could enhance CD8+ T-cell cytotoxicity. Altogether, these data pave the way for therapy targeting YAP1 in ECM-myCAF in HGSOC.
Agile THz-range spectral multiplication of frequency combs using a multi-wavelength laser
Authors: Abdollahi Shahab , Ladouce Mathieu , Marin-Palomo Pablo , Virte Martin
Published: February 2024

A breakthrough technology, on-chip frequency comb sources offer broadband combs while being compact, energy-efficient, and cost-effective solutions for various applications from lidar to telecommunications. Yet, these sources encounter a fundamental trade-off between controllability and bandwidth: broadband combs, generated in microresonators, lack free-spectral range or spectral envelope control, while combs generated with electro-optic modulators can be carefully tailored but are limited in bandwidth. Here, we overcome this trade-off through agile spectral multiplication of narrowband combs. Exploiting the nonlinear dynamics of a multi-wavelength laser under modulated optical injection, we achieve spectral multiplication at frequency offsets from 26 GHz to 1.3 THz. Moreover, on-chip control allows for nano-second switching of the frequency offset. Compatible with generic platforms, our approach can be scaled up to cover several THz. When combined with THz photomixers, our system could enable low-cost, compact, and power-efficient THz comb sources, paving the way towards a new generation of THz applications.
Manufacturing of high strength and high conductivity copper with laser powder bed fusion
Authors: Liu Yingang , Zhang Jingqi , Niu Ranming , Bayat Mohamad , Zhou Ying , Yin Yu , Tan Qiyang , Liu Shiyang , Hattel Jesper Henri , Li Miaoquan , Huang Xiaoxu , Cairney Julie , Chen Yi-Sheng , Easton Mark , Hutchinson Christopher , Zhang Ming-Xing
Published: February 2024

Additive manufacturing (AM), known as 3D printing, enables rapid fabrication of geometrically complex copper (Cu) components for electrical conduction and heat management applications. However, pure Cu or Cu alloys produced by 3D printing often suffer from either low strength or low conductivity at room and elevated temperatures. Here, we demonstrate a design strategy for 3D printing of high strength, high conductivity Cu by uniformly dispersing a minor portion of lanthanum hexaboride (LaB6) nanoparticles in pure Cu through laser powder bed fusion (L-PBF). We show that trace additions of LaB6 to pure Cu results in an improved L-PBF processability, an enhanced strength, an improved thermal stability, all whilst maintaining a high conductivity. The presented strategy could expand the applicability of 3D printed Cu components to more demanding conditions where high strength, high conductivity and thermal stability are required.
Diverse volcanism and crustal recycling on early Mars
Authors: Michalski Joseph R. , Deanne Rogers A. , Edwards Christopher S. , Cowart Aster , Xiao Long
Published: February 2024

The relatively well-preserved ancient crust of Mars provides a natural window into early planetary evolution not available on Earth due to sustained tectonic recycling and erosion on this planet. Mars has generally been considered a one-plate basaltic planet, though recent evidence suggests magmatic evolution resulting in felsic crust might have occurred sporadically. Here we show multiple lines of evidence for diverse volcanism and complex volcanotectonics in the southern highlands of Mars within and around the ∼3.5–4-billion-year-old Eridania basin. Infrared remote sensing reveals bimodal volcanism consisting of olivine-bearing basalts and voluminous, widespread dacitic (64–69% SiO2, and possibly higher) volcanic deposits within a region of high crustal potassium. The diverse igneous compositions are associated with an extraordinary number and morphological range of volcanic structures, including domes, stratovolcanoes, calderas and pyroclastic shields occurring proximal to large (hundreds of kilometres in diametre) basins within the Eridania region. The 2–4 km-deep topographically concave-up basins have crustal thicknesses 10–20 km thinner than adjacent terrain and disrupt patterns of deeply seated remnant crustal magnetism. The Eridania basins may represent ancient episodes of crustal recycling via lithospheric delamination in which altered, hydrated volcanic materials were cycled downward and melted resulting in magmatic evolution analogous to pre-plate tectonic processes on the Archaean Earth.
Basal ganglia–spinal cord pathway that commands locomotor gait asymmetries in mice
Authors: Cregg Jared M. , Sidhu Simrandeep K. , Leiras Roberto , Kiehn Ole
Published: February 2024

The basal ganglia are essential for executing motor actions. How the basal ganglia engage spinal motor networks has remained elusive. Medullary Chx10 gigantocellular (Gi) neurons are required for turning gait programs, suggesting that turning gaits organized by the basal ganglia are executed via this descending pathway. Performing deep brainstem recordings of Chx10 Gi Ca2+ activity in adult mice, we show that striatal projection neurons initiate turning gaits via a dominant crossed pathway to Chx10 Gi neurons on the contralateral side. Using intersectional viral tracing and cell-type-specific modulation, we uncover the principal basal ganglia–spinal cord pathway for locomotor asymmetries in mice: basal ganglia → pontine reticular nucleus, oral part (PnO) → Chx10 Gi → spinal cord. Modulating the restricted PnO → Chx10 Gi pathway restores turning competence upon striatal damage, suggesting that dysfunction of this pathway may contribute to debilitating turning deficits observed in Parkinson’s disease. Our results reveal the stratified circuit architecture underlying a critical motor program.
Ancient and modern DNA track temporal and spatial population dynamics in the European fallow deer since the Eemian interglacial
Authors: Baker K. H. , Gray H. W. I. , Lister A. M. , Spassov N. , Welch A. J. , Trantalidou K. , De Cupere B. , Bonillas E. , De Jong M. , Çakırlar C. , Sykes N. , Hoelzel A. R.
Published: February 2024

Anthropogenic factors have impacted the diversity and evolutionary trajectory of various species. This can be through factors such as pressure on population size or range, habitat fragmentation, or extensive manipulation and translocation. Here we use time-calibrated data to better understand the pattern and processes of evolution in the heavily manipulated European fallow deer (Dama dama). During the Pleistocene, fallow deer had a broad distribution across Europe and were found as far north as Britain during the Eemian interglacial. The last glacial period saw fallow deer retreat to southern refugia and they did not disperse north afterwards. Their recolonisation was mediated by people and, from northern Europe and the British Isles, fallow deer were transported around the world. We use ancient and modern mitochondrial DNA (mtDNA) and mitogenomic data from Eemian Britain to assess the pattern of change in distribution and lineage structure across Europe over time. We find founder effects and mixed lineages in the northern populations, and stability over time for populations in southern Europe. The Eemian sample was most similar to a lineage currently in Italy, suggesting an early establishment of the relevant refuge. We consider the implications for the integration of anthropogenic and natural processes towards a better understanding of the evolution of fallow deer in Europe.