bluuebunny/crossref_metadata_2025 huggingface dataset:
An ultra-dense linkage map identified quantitative trait loci corresponding to fruit quality- and size-related traits in red goji berry
Authors: Rehman Fazal , Gong Haiguang , Ma Yun , Zeng Shaohua , Ke Danmin , Yang Chao , Zhao Yuling , Wang Ying
Published: September 2024

Goji berries are a small-fruited shrub with industrial importance whose fruit considered beneficial in both fresh and dried forms. Current germplasms of goji berries include small fruits with a short shelf life, less sweet and bitter taste, and a lack of appropriate genetic information. This study aimed to employ whole genome resequencing to generate an ultra-dense bin linkage map and to elucidate the genetic basis of goji fruit quality and size using quantitative trait loci (QTL) mapping analysis in a cross-pollinated hybrid population. To achieve this goal, human sensory tests were carried out to determine the bitter taste (BT) and sweet taste (ST), and to quantify the soluble solid content (SSC), fruit firmness (FF), and fruit size-related traits of fresh goji fruits over three or four years. The results revealed that the goji bin linkage map based on resequencing spanned a total length of 966.42 cM and an average bin interval of 0.03 cM. Subsequent variant calling and ordering resulted in 3,058 bins containing 35,331 polymorphic markers across 12 chromosomes. A total of 99 QTLs, with individual loci in different environments explaining a phenotypic variance of 1.21-16.95% were identified for the studied traits. Ten major effects, including colocalized QTLs corresponding to different traits, were identified on chromosomes 1, 3, 5, 6, 7, and 8, with a maximum Logarithm of Odds (LOD) of 29.25 and 16.95% of explained phenotypic variance (PVE). In addition, four stable loci, one for FF, one for fruit weight (FW), and two for fruit shape index (FSI), were mainly mapped on chromosomes 5, 6, and 7, elucidating 2.10-16.95% PVE. These findings offer valuable insights into the genetic architecture of goji fruit traits along with identified specific loci and markers to further improve and develop sweeter, less bitter and larger fruited goji berry cultivars with extended shelf life.
Arbuscular mycorrhizal fungi improve the disease resistance of Lycium barbarum to root rot by activating phenylpropane metabolism
Authors: Li Nan , Chen Wei , Wang Bin , Zhang Chongqing , Wang Yupeng , Li Ruiyun , Yan Yuke , He Jing
Published: September 2024

Root rot is one of the common diseases of Lycium barbarum. Pathogens can cause devastating disasters to plants after infecting host plants. This study investigated the effect of arbuscular mycorrhizal fungi (AMF) Rhizophagus intraradices inoculation on phenylpropane metabolism in L. barbarum and evaluated its resistance to root rot. The experiment was set up with AMF inoculation treatments (inoculated or not) and root rot pathogen-Fusarium solani inoculation treatments (inoculated or not). The results showed that AMF was able to form a symbiosis with the root system of L. barbarum, thereby promoting plant growth significantly and increasing plants’ resistance to disease stress. The plant height of AMF-colonized L. barbarum increased by 24.83% compared to non-inoculated diseased plants. After inoculation with AMF, the plant defense response induced by pathogen infection was stronger. When the enzyme activity of the leaves reached the maximum after the onset of mycorrhizal L. barbarum, phenylalanine ammonia-lyase, cinnamic acid-4-hydroxylase, and 4-coumaric acid-CoA ligase increased by 3.67%, 31.47%, and 13.61%, respectively, compared with the non-inoculated diseased plants. The products related to the lignin pathway and flavonoid pathway downstream of phenylpropane metabolism such as lignin and flavonoids were also significantly increased by 141.65% and 44.61% compared to nonmycorrhizal diseased plants. The activities of chitinase and β-1,3-glucanase increased by 36.00% and 57.96%, respectively. The contents of salicylic acid and jasmonic acid were also 17.7% and 31.63% higher than those of nonmycorrhizal plants in the early stage of plant growth, respectively. The results indicated that AMF significantly promoted plant growth and enhanced disease resistance by increasing enzyme activities and the production of lignin and flavonoids.
Effect of social support on Muslim women’s sporting activities: mediating effect of psychological adjustment
Authors: Chen Nan , Qiao Fengjie
Published: September 2024

Objective

This study explores the relationship between social support and sporting activities of Muslim women and constructs a mediation model through role of psychological adjustment.

Methods

Using stratified cluster sampling, 301 Muslim women were measured in group psychology using the Social Support Scale and the Sports Activities and Psychological Adjustment Scale. The statistical software SPSS 24.0 and SPSS PROCESS 3.3 were used for statistical processing. The common-method variation test was carried out using the Harman single-factor control test. Finally, the Bootstrap sampling test method and process plug-in were used to test the significance of the intermediary effect.

Results

(1) Social support has a significant predictive effect on sports activities (β = 0.32, p < 0.001); (2) psychological adjustment (β = 0.552, p < 0.001) mediates the relationship between social support and sporting activities [social support → psychological adjustment → sporting activities (95% Cl, 0.093, 0.323)].

Conclusion

Social support positively influences sporting participation among Muslim women, and psychological adjustment mediates this relationship. Strengthening social support for Muslim women can enhance their psychological adjustment, thereby improving their participation in sporting activities and offering valuable theoretical and practical guidance.
Campus landscape types and pro-social behavioral mediators in the psychological recovery of college students
Authors: Xu Yi , Wang Tingting , Wang Jinsong , Tian Hongtao , Zhang Ruixin , Chen Yixuan , Chen Hong
Published: September 2024

Introduction

Physical and mental health problems of college students are becoming more prominent, and contact with nature has a positive effect on physical and mental health. This paper investigates the psychological recovery effect of different types of campus green space landscape on college students. From the perspective of college students’ perception of campus landscape types, the green space, blue space, gray space and movement space of three universities in Anhui Province are investigated.

Methods

Through choose campus landscape types and questionnaires, structural equation modeling (SEM) and mediation modeling were constructed on the role of college students’ perception of campus landscape types on psychological recovery.

Results

It was found that the level of landscape type perception had a significant effect on the effect of psychological recovery and the generation of pro-social behavior, with no significant gender difference, while psychological recovery also had a positive effect on the generation of pro-social behavior. The study also found that campus landscape type not only directly affect students psychological recovery, but also promote psychological recovery through the mediating role of pro-social behavior.

Discussion

The study reveals the effects of campus landscape type on college students’ psychological recovery, and pro vides a basis for planning campus of different types.
Healthcare utilization and economics evaluation of paliperidone palmitate once-monthly in schizophrenia: a one-year, real-world, and retrospective mirror image study in China
Authors: Zhou Yixiang , Chen Binbin , Huang Yinghua
Published: September 2024

Background

Investigation and analysis of the changes in healthcare resources and burden of schizophrenia in the real world before and after switching from oral antipsychotics (OAPs) to paliperidone palmitate once-monthly (PP1M) could provide evidence to clinicians and patients for choosing treatment modality and data support for health policy optimization

Methods

The first dosage of PP1M was set as mirror point, and patients with mirror point between January 2020 and June 2022 were recruited in the study. The differences in treatment patterns, healthcare resource utilization, and costs within one year before and after the mirror point were compared.

Results

A total of 72 patients transitioning from OAPs to PP1M (mean age, 35.33 years; 43.06% female) were included in the study. Of the 72 patients, the application of PP1M resulted in a significant reduction in the hospitalization times, emergency room visits, and direct medical costs (P < 0.001), while the pharmacy cost and total cost increased by 222.57% (P < 0.001) and 16.35% (P < 0.001), respectively; PP1M accounted for 88.48% of the pharmacy cost. For patients with ≥1 hospitalization during the OAPs phase (n = 25), the number of hospitalizations, hospitalization days and hospitalization expenses decreased by more than 90% (P < 0.001). Total one-year expenses decreased by 37.67% (P < 0.001), and pharmacy expenses increased by 185.21% (P < 0.001). For patients with no hospitalizations during the OAPs phase (n = 47), emergency and outpatient visits decreased by 70% (P < 0.001) and 30.27% (P < 0.05), respectively, while the total cost increased by 117.56% (P < 0.001), and the pharmacy cost increased by 260.15% (P < 0.001) after initiation of PP1M treatment.

Conclusion

After the transition to PP1M, the number of hospitalizations and outpatient and emergency department visits reduced, and healthcare resources were conserved. Switching to PP1M may be more economically beneficial for patients with prior hospitalizations while on OAP regimens. The high price of PP1M might be an obstacle to its widespread use.
Minimum acceptable diet use and its associated factors among children aged 6–23 in Ghana: a mixed effect analysis using Ghana Demographic and Health Survey
Authors: Tekeba Berhan , Workneh Belayneh Shetie , Zegeye Alebachew Ferede , Gonete Almaz Tefera , Zeleke Gebreeyesus Abera , Tamir Tadesse Tarik
Published: September 2024

Introduction

Inappropriate feeding practices are a major contributor to child malnutrition. To monitor the feeding practices of young children, current and frequent studies are required. However, as far as our searches are concerned, there is a scarcity of up-to-date information on attainment of the minimum acceptable diet and its predictors in the study area. Therefore, this study aimed to assess the magnitude of attainment of the minimum acceptable diet and its associated factors among children aged 6–23 in Ghana by using the most recent data.

Methods

Secondary data analysis was conducted based on the demographic and health survey data conducted in Ghana in 2022. A total weighted sample of 2,621 children aged 6–23 months in the 5 years preceding the survey was included in this study. A multi-level logistic regression model was used to identify the determinants of the minimum acceptable diet. The adjusted odds ratio at 95% Cl was computed to assess the strength and significance of the association between explanatory and outcome variables. Factors with a p-value of <0.05 are declared statistically significant.

Results

The national prevalence of the attainment of the minimum acceptable diet in Ghana was 26.40% (95% CI: 24.82–28.06). Child from mother with higher education (AOR = 1.96; 95% CI: 1.56–3.31) and father with higher education (AOR = 1.59; 95% CI: 1.04–2.41), Children having postnatal visit (AOR = 1.29; 95% CI: 1.03–1.62), being in the child age of 9–11 months (AOR = 2.09; 95% CI: 1.42–5.03) and 12–23 months (AOR = 3.62; 95% CI: 2.61–5.03), being in a middle (AOR = 1.66; 95% CI: 1.14–3.06), and rich wealth quintile (AOR = 2.06; 95% CI: 1.37–3.10), breastfed children (AOR = 3.30; 95% CI: 2.38–4.56), being in a high-community poverty (AOR = 0.65; 95% CI: 0.44–0.96), and being in the Savannah region (AOR = 0.32; 95% CI: 0.16–0.67) were factors significantly associated with the minimum acceptable diet use.

Conclusion

Many children are still far behind in meeting the minimum acceptable diet in Ghana as per 90% of WHO-recommended coverage. Measures should be taken to optimize the minimum acceptable diet attainment in the country. Thus, policymakers, the government, and other relevant authorities should focus on the early initiation of complementary feeding, the Savannah region, further empowering women, and enhancing breast-feeding and household wealth status.
Study protocol of a proposed Neurofeedback-Assisted Mindfulness Training Program on symptoms of anxiety and psychological distress associated with smartphone use in young adults: a randomized controlled trial
Authors: Fernandez-Crespo Mei , Recio-Rodriguez Jose I. , Lee Hsin-Chien , Alonso-Dominguez Rosario , Montejo Angel L. , Hernandez-Gonzalez Laura , Iglesias Sierra Virginia , Rihuete-Galve Maria I.
Published: September 2024

Background

Nomophobia is a specific phobia characterized by the appearance of anxiety, nervousness, discomfort and distress when the mobile phone is not used and is considered an emerging public health problem because of the negative consequences on the physical and mental health of young people and adolescents, especially women. Neurofeedback-Assisted Mindfulness Training Programs may prove beneficials for improving self-control abilities, a key ability in addressing addictive behaviors. The main objective of this study is to evaluate the impact, in a young population aged 18–35 years, of an intervention based on Neurofeedback-Assisted Mindfulness Training Program (NAMTP) on disorders associated with problematic use of mobile phones. The effect of the intervention on the total score in the nomophobia test and habits of internet and social network use, as well as on signs of depression, anxiety and stress will be analyzed. As a secondary objective, the effect of the intervention on signs of insomnia will be analyzed.

Methods and design

Randomized, controlled clinical-trial with two-parallel groups. 40 young adults (18–35 years) will be included and randomly assigned to Intervention Group-NAMTP or Control Group (CG). The NAMTP will include a total of 25 sessions (2-3/week) during 3-months. Each session will have a duration of 10/15 min. The instrument to be used for the neurofeedback sessions is MUSE® (InteraXon Inc.). Study variables will be collected at the baseline visit and at the final visit (3-months after randomization). During these visits, questionnaires will be administered to evaluate the main and secondary variables that will include the Smartphone Addiction Scale-Short Version, Nomophobia Questionnaire, Depression, Anxiety and Stress Scale 21-item (DASS-21) and Athens Insomnia Scale.

Discussion

This trial will make an important contribution to the need for evidence of effective education programs and other primary care interventions through new non-invasive interventions in reducing the risk of developing addictions to new technologies and alleviating the symptoms of discomfort associated with this problem.

Ethics and dissemination

The project was approved by the Clinical Research Ethics Committee of the Salamanca Health Area (CEIm Code: PI 2023 071340).

Clinical trial registration

ClinicalTrials.gov, http://www.Clinicaltrials.gov/ct2/show/NCT06188910.
The association between health literacy and screening for disease-specific complications among community-dwelling adults with diabetes
Authors: Nari Fatima , Jun Jae Kwan , Oh Kyoung Hee , Jeong Wonjeong
Published: September 2024

Introduction

Diabetic retinopathy and nephropathy are examples of complications of uncontrolled diabetes. We hypothesized that health literacy has a defining role in understanding the importance of attending routine screening for diabetes complications. Therefore, our study investigated the relationship between verbal health literacy (VHL) and written health literacy (WHL) and screening for disease-specific complications in individuals with diabetes.

Methods

Cross-sectional data on 28,210 participants with diabetes was derived from the 2021 Korean Community Health Survey. Adjusted multiple logistic regression analysis was employed to investigate the association between VHL and WHL and diabetes complication screening. Further analysis was also carried out to further comprehend the relationship between those two forms of health literacy and other factors with diabetic retinopathy and nephropathy screening.

Results

Compared to those with high VHL, participants with low VHL had lower odds of diabetes complication screening; OR 0.89 (95% CI 0.84—0.95). The same was true for WHL, those who were uninterested reported the lowest odds ratio; OR 0.73 (95% CI 0.69—0.78), followed by low WHL; OR 0.88 (95% CI 0.82—0.94), of undergoing diabetes complication screening, when compared to individuals with high WHL. Our subgroup analysis presented similar results for diabetic nephropathy and retinopathy with both VHL and WHL.

Conclusion

Among individuals with diabetes, limited VHL and WHL was significantly associated with lower odds of diabetes complication screening. Interventions aimed at improving health literacy and associated health outcomes in the community setting are warranted.
The green transformation of agricultural production: evaluating the spatial role of agricultural industrial structure innovation in Guangdong, China
Authors: Zhang Weikun , Li Zheng , Wagan Shoaib Ahmed , Tan Shiwei
Published: September 2024

Introduction

The primary function and spatial layout of agriculture are essential foundations and prerequisites for achieving green development. This paper explores the growth drivers for the green transformation of agricultural production in Guangdong, China, aiming to guide the continuous green and low-carbon development of agricultural productivity in Guangdong.

Methods

Based on the panel data of 21 prefecture-level cities in Guangdong, China, from 2004 to 2022, this paper uses DEA-BCC and DEA-Malmquist to measure the agricultural green total factor productivity (AGTFP) and its decomposition efficiency in Guangdong China. The SDM is employed to analyze its spatial spillover roles of the agricultural industrial structure (AIS) innovation.

Results

The results show that the AGTFP varies with an overall fluctuating upward trend, but the imbalance of regional differences still exists, characterized as “Pearl River Delta > Eastern Wing > Western Wing > Mountainous Area,” indicating the siphoning role from Pearl River Delta. There is a strong spatial correlation between the AGTFP and the AIS.

Discussion

Our evidence suggests that the AIS innovation improves the AGTFP and can have positive spatial consequences in neighboring areas. Besides, human capital hinders AGTFP, accounting for the self-exploitation in agricultural production driven by the excessive labor force input. Our findings highlight the spatial impact of the AIS innovation on the AGTFP when greening agricultural production.
Enhancing educational experience through establishing a VR database in craniosynostosis: report from a single institute and systematic literature review
Authors: Saemann Attill , Schmid Sina , Licci Maria , Zelechowski Marek , Faludi Balazs , Cattin Philippe C. , Soleman Jehuda , Guzman Raphael
Published: September 2024

Background

Craniosynostosis is a type of skull deformity caused by premature ossification of cranial sutures in children. Given its variability and anatomical complexity, three-dimensional visualization is crucial for effective teaching and understanding. We developed a VR database with 3D models to depict these deformities and evaluated its impact on teaching efficiency, motivation, and memorability.

Methods

We included all craniosynostosis cases with preoperative CT imaging treated at our institution from 2012 to 2022. Preoperative CT scans were imported into SpectoVR using a transfer function to visualize bony structures. Measurements, sub-segmentation, and anatomical teaching were performed in a fully immersive 3D VR experience using a headset. Teaching sessions were conducted in group settings where students and medical personnel explored and discussed the 3D models together, guided by a host. Participants’ experiences were evaluated with a questionnaire assessing understanding, memorization, and motivation on a scale from 1 (poor) to 5 (outstanding).

Results

The questionnaire showed high satisfaction scores (mean 4.49 ± 0.25). Participants (n = 17) found the VR models comprehensible and navigable (mean 4.47 ± 0.62), with intuitive operation (mean 4.35 ± 0.79). Understanding pathology (mean 4.29 ± 0.77) and surgical procedures (mean 4.63 ± 0.5) was very satisfactory. The models improved anatomical visualization (mean 4.71 ± 0.47) and teaching effectiveness (mean 4.76 ± 0.56), with participants reporting enhanced comprehension and memorization, leading to an efficient learning process.

Conclusion

Establishing a 3D VR database for teaching craniosynostosis shows advantages in understanding and memorization and increases motivation for the study process, thereby allowing for more efficient learning. Future applications in patient consent and teaching in other medical areas should be explored.
Fasting triglyceride concentrations are associated with markers of lipid metabolism and glucose homeostasis in healthy, non-obese dogs in lean and overweight condition
Authors: Gomez-Fernandez-Blanco Carlos , Peeters Dominique , Farnir Frédéric , Höglund Katja , Gouni Vassiliki , Wiberg Maria , Lundgren Willesen Jakob , Hanås Sofia , McEntee Kathleen , Tiret Laurent , Häggström Jens , Lohi Hannes , Chetboul Valérie , Fredholm Merete , Seppälä Eija , Lequarré Anne-Sophie , German Alexander James , Merveille Anne-Christine
Published: September 2024

Serum triglyceride concentrations increase in dogs with obesity, which is typically assessed by body condition score (BCS), however little is known about changes that take place in non-obese dogs in overweight condition. Further, the associations of triglyceride levels with other markers of energy homeostasis are poorly characterised in healthy animals. The present study aimed to evaluate associations between both BCS and triglyceride concentrations with other markers of lipid and glucose metabolism in healthy, non-obese dogs, as well as to assess whether these markers change significantly in non-obese dogs with overweight as compared to their lean counterparts. Serum concentrations of cholesterol, free fatty acids, triglycerides, insulin, glucose and fructosamine were measured in 532 healthy, client-owned dogs, assigned either to ‘lean’ (BCS: 3–5) or ‘overweight’ (BCS: 6–7) categories. Generalised linear mixed models were used to assess associations between BCS categories, triglyceride concentrations and other variables, correcting for the effect of breed. Compared with lean dogs, overweight dogs had a greater serum cholesterol concentration (95% CI, 5.3–6.2 mmol/L or 205–237 mg/dL versus 5.1–5.4 mmol/L or 198–210 mg/dL, p = 0.0032), insulin concentration (95% CI, 17.5–22.1 μU/ml versus 16.7–18.0 μU/ml, p = 0.0374) and were older (95% CI, 4.0–5.3 versus 3.4–3.7 years, p = 0.0005). Triglyceride concentrations were positively associated with fructosamine (r2 = 0.31, p = 0.0012), cholesterol (r2 = 0.25, p < 0.0001), insulin (r2 = 0.14, p = 0.0030) and glucose (r2 = 0.10, p = 0.0014) concentrations, and negatively associated with free fatty acid concentrations (r2 = 0.11, p < 0.0001). However, there was no association between triglyceride concentrations and age. In conclusion, both BCS and triglyceride concentrations were associated with other markers of glucose and lipid metabolism in non-obese healthy dogs, amongst which those with overweight showed metabolic changes as compared to their lean counterparts. Triglyceride concentrations were associated with an increase in insulin and fructosamine concentrations that might reflect an early-phase impairment in glucose tolerance which, surprisingly, was concurrent with lower basal free fatty acid concentrations.
Deep learning-based ultrasonographic classification of canine chronic kidney disease
Authors: Yu Heejung , Lee In-Gyu , Oh Jun-Young , Kim Jaehwan , Jeong Ji-Hoon , Eom Kidong
Published: September 2024

Objectives

In veterinary medicine, attempts to apply artificial intelligence (AI) to ultrasonography have rarely been reported, and few studies have investigated the value of AI in ultrasonographic diagnosis. This study aimed to develop a deep learning-based model for classifying the status of canine chronic kidney disease (CKD) using renal ultrasonographic images and assess its diagnostic performance in comparison with that of veterinary imaging specialists, thereby verifying its clinical utility.

Materials and methods

In this study, 883 ultrasonograms were obtained from 198 dogs, including those diagnosed with CKD according to the International Renal Interest Society (IRIS) guidelines and healthy dogs. After preprocessing and labeling each image with its corresponding IRIS stage, the renal regions were extracted and classified based on the IRIS stage using the convolutional neural network-based object detection algorithm You Only Look Once. The training scenarios consisted of multi-class classification, categorization of images into IRIS stages, and four binary classifications based on specific IRIS stages. To prevent model overfitting, we balanced the dataset, implemented early stopping, used lightweight models, and applied dropout techniques. Model performance was assessed using accuracy, recall, precision, F1 score, and receiver operating characteristic curve and compared with the diagnostic accuracy of four specialists. Inter- and intra-observer variabilities among specialists were also evaluated.

Results

The developed model exhibited a low accuracy of 0.46 in multi-class classification. However, a significant performance improvement was observed in binary classifications, with the model designed to distinguish stage 3 or higher showing the highest accuracy of 0.85. In this classification, recall, precision, and F1 score values were all 0.85, and the area under the curve was 0.89. Compared with radiologists, whose accuracy ranged from 0.48 to 0.62 in this experimental scenario, the AI model exhibited superiority. Intra-observer reliability among radiologists was substantial, whereas inter-observer variability showed a moderate level of agreement.

Conclusions

This study developed a deep-learning framework capable of reliably classifying CKD IRIS stages 3 and 4 in dogs using ultrasonograms. The developed framework demonstrated higher accuracy than veterinary imaging specialists and provided more objective and consistent interpretations. Therefore, deep-learning-based ultrasound diagnostics are potentially valuable tools for diagnosing CKD in dogs.
Supplementation of sperm cooling medium with Eurycoma longifolia extract enhances native Thai chicken sperm quality and fertility potential
Authors: Koedkanmark Thirawat , Ratchamak Ruthaiporn , Authaida Supakorn , Boonkum Wuttigrai , Semaming Yoswaris , Chankitisakul Vibuntita
Published: September 2024

Cooled semen storage methods result in oxidative stress generated by an imbalance between oxidation rates, specifically reactive oxygen species production, and sperm cell antioxidants, leading to degradation of semen quality. We aimed to investigate the impact of adding Eurycoma longifolia (EL) extract as an antioxidant supplement in semen storage medium (IGGKPh semen extender) on semen quality and fertility potential. EL extract at concentrations of 5, 10, 15, and 20 mg/mL was assessed for its antioxidant capacity in IGGKPh semen extender. Our findings revealed that the total phenolic content in the EL extract did not vary significantly across the various concentrations and temperatures tested. However, incubation at 5°C was found to be the most effective temperature for increasing the EL extract antioxidant capacity as assessed via the 2,2-diphenyl-1-picrylhydrazyl inhibition assay in a dose-dependent manner. Supplementation of the IGGKPh semen extender with 15 mg/mL EL extract was found to enhance semen quality during cold storage for up to 48 h (p < 0.05), as evidenced by decreased malondialdehyde levels in cooled semen (p < 0.05). However, antioxidant enzyme activities showed no significant differences among the various experimental groups (p > 0.05). The fertility test showed that the 15 mg/mL EL extract group stored for 24 h had a higher percentage than the control group (p < 0.05). However, there was no significant difference in percentage between the two groups at 48 h of storage (p > 0.05). The hatchability showed no significant difference in both 24 and 48-h storage periods (p > 0.05). Our results indicated that supplementing the IGGKPh semen extender with 15 mg/mL EL extract may positively influence semen quality during storage, suggesting potential applications for enhancing semen quality.
Correction: Kim et al. Development of Ethyl Formate Disinfestation Treatment Methods for the Prevention of the Introduction and Establishment of Exotic Insect Pests in Greenhouse Cultivation. Agriculture 2023, 13, 2251
Authors: Kim Kyeongnam , Kim Dongbin , Kim Chaeeun , Kim Donghyeon , Kim Byeongjun , Lee Byung-Ho , Lee Sung-Eun
Published: September 2024

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Performance Study of a Chain–Spoon Seed Potato Discharger Based on DEM-MBD Coupling
Authors: Xing Wei , Zhang Hua , Sun Wei , Li Hui , Liu Xiaolong , Li Hongling , Chen Yangzhou , Lu Yonggang
Published: September 2024

To address the issues of the poor filling and clearing efficiency of spoon–chain potato seed dischargers, an optimization design was implemented in this study. Based on the motion characteristics of potatoes during the filling and transport processes, an inclination angle was set for the seed spoon cavity, and a seed-clearing brush was installed at the top of the seed discharger. A DEM-MBD coupled simulation model of the seed discharger was constructed. The working speed of the driving sprocket, the inclination angle of the seed spoon cavity, and the seed holding height were used as experimental factors, while the single-seed qualification rate, missed seed rate, and over-seeding rate were used as evaluation indices to conduct a quadratic regression orthogonal rotation combination experiment. This determined the optimal technical parameter combination for the best working performance. Based on the results of the DEM-MBD coupled simulation experiments, a response surface optimization test was conducted. The results showed that the optimal working performance was achieved when the working speed of the driving sprocket was 43 rpm, the inclination angle of the seed spoon cavity was 15°, and the seed holding height was 0.2 m. Under these conditions, the single-seed qualification rate was 95.28%, the missed seed rate was 0.92%, and the over-seeding rate was 3.80%. Further soil bin tests confirmed that, under the optimal working parameters, the relative deviations of all test indices from the response surface optimization test results were less than 2%. The research results provide new insights for the optimization design of spoon–chain potato seed-metering devices.
Exploring the Role of Biostimulants in Sweet Cherry (Prunus avium L.) Fruit Quality Traits
Authors: Afonso Sílvia , Oliveira Ivo , Ribeiro Carlos , Vilela Alice , Meyer Anne S. , Gonçalves Berta
Published: September 2024

Sweet cherries are among consumers’ preferred fresh fruits, known for their attractive organoleptic properties and high nutritional value. Agronomical practices, which are now shifting to more environmentally sustainable options, can influence several key quality traits of sweet cherries. In this context, reducing conventional agrochemicals and increasing the application of preharvest biostimulants has emerged as an innovative strategy. This approach can not only enhance cherry production and quality but also ensure the economic and environmental sustainability of the cherry supply chain. Hence, this work is aimed at studying the effect of the application of two concentrations of glycine betaine (GB) and Ecklonia maxima-based (EM) biostimulants, and their combination, in two cultivars of sweet cherry: the early-maturing ‘Early Bigi’ and the late-maturing ‘Lapins’, both grafted onto SL-64 rootstock. Evaluated parameters included fruit weight and dimensions, color, firmness, total soluble solids (TSS), titratable acidity (TA), phenolic and anthocyanin contents, and sensory profile. Key findings highlight that, with a few exceptions, biostimulant treatments had a positive impact on the studied parameters, although the responses varied between cultivars. For instance, fruit size increased by 13.41% in ‘Early Bigi’ and 47.20% in ‘Lapins’. Additionally, reduced color values, coupled with higher TSS/TA ratios, indicate advanced fruit maturation, which could allow for an earlier harvest. The total phenolic content rose by 56.88% in ‘Early Bigi’ and 30.24% in ‘Lapins’, while anthocyanin levels surged by 88.28% and 36.10%, respectively. Fruit firmness also improved following biostimulant application. Sensory analysis further revealed enhancements in key descriptors such as “overall aspect”, “firmness”, and “cherry flavor”, underscoring the beneficial effects of these treatments. These combined results indicate that the preharvest application of glycine betaine or Ecklonia maxima-based (EM) biostimulants significantly improves key quality traits of sweet cherries. This approach offers benefits not only from a commercial perspective but also for the sweet cherry supply chain sustainability by reducing the application of chemical-based products and replacing them with ecofriendly substances while enhancing the quality of the fruit.
Effect of Moringa oleifera Leaf Powder Supplementation on Growth Performance, Digestive Enzyme Activity, Meat Quality, and Cecum Microbiota of Ningdu Yellow Chickens
Authors: Song Qiongli , Zou Zhiheng , Chen Xiaolian , Ai Gaoxiang , Xiong Pingwen , Song Wenjing , Liu Guohua , Zheng Aijuan , Chen Jiang
Published: September 2024

This study aimed to investigate the impact of dietary supplementation with Moringa oleifera leaf powder (MOLP) on the growth performance, digestive enzyme activity, meat quality, and cecum microbiota of Ningdu yellow chickens. A total of 300 78-day-old Ningdu yellow chickens with similar initial body weights were randomly distributed into five treatments consisting of six replicates of 10 birds. The control group (M0) was fed a basal diet, and the experimental groups were fed diets supplemented with 0.5% (M0.5), 1% (M1), 2% (M2), and 4% (M4) of MOLP, respectively. Our results showed that dietary supplementation with 2% MOLP significantly (p < 0.05) decreased the feed to gain (F/G) and showed a quadratic (p < 0.05) decrease with the level of MOLP. Dietary supplementation with 1~4% MOLP resulted in a significant increase (p < 0.05) in serum total superoxide dismutase (T-SOD) activity and total antioxidant capacity (T-AOC). Furthermore, both serum T-SOD and T-AOC exhibited linear and quadratic increases (p < 0.01) in response to the supplementation with MOLP in the diets. Dietary supplementation with 1~4% MOLP significantly (p < 0.05) decreased serum uric acid (UA) level. Additionally, 4% MOLP significantly (p < 0.05) decreased triglycerides (TG), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels, and showed linear and quadratic effects. The activity of lipase in the duodenum showed a linear decreasing trend (p < 0.05) with the level of MOLP, while the activities of α-amylase (both in duodenum and jejunum) showed a linear and quadratic increasing trend (p < 0.05). In addition, there was a linear decrease response in abdominal fat (p < 0.05) to MOLP supplementation levels in the diets. In terms of meat quality, dietary supplementation with 4% MOLP significantly reduced (p < 0.05) the L*45 min and L*24 h values of the breast muscle, and drip loss had a linear decreasing trend (p < 0.05). In terms of cecum microbiota, dietary supplementation with 1~4% MOLP significantly increased the Bacteroidota abundance but decreased the Firmicutes abundance (p < 0.05). Overall, dietary supplementation with MOLP improved the growth performance and meat quality of Ningdu yellow chickens through improving the antioxidant function, intestinal digestive enzyme activity, and the cecal microbial structure. The optimum level of MOLP in the diet of Nindu yellow chicken is recommended to be 2.59%.
Prediction of Useful Eggplant Seedling Transplants Using Multi-View Images
Authors: Yuan Xiangyang , Liu Jingyan , Wang Huanyue , Zhang Yunfei , Tian Ruitao , Fan Xiaofei
Published: September 2024

Traditional deep learning methods employing 2D images can only classify healthy and unhealthy seedlings; consequently, this study proposes a method by which to further classify healthy seedlings into primary seedlings and secondary seedlings and finally to differentiate three classes of seedling through a 3D point cloud for the detection of useful eggplant seedling transplants. Initially, RGB images of three types of substrate-cultivated eggplant seedlings (primary, secondary, and unhealthy) were collected, and healthy and unhealthy seedlings were classified using ResNet50, VGG16, and MobilNetV2. Subsequently, a 3D point cloud was generated for the three seedling types, and a series of filtering processes (fast Euclidean clustering, point cloud filtering, and voxel filtering) were employed to remove noise. Parameters (number of leaves, plant height, and stem diameter) extracted from the point cloud were found to be highly correlated with the manually measured values. The box plot shows that the primary and secondary seedlings were clearly differentiated for the extracted parameters. The point clouds of the three seedling types were ultimately classified directly using the 3D classification models PointNet++, dynamic graph convolutional neural network (DGCNN), and PointConv, in addition to the point cloud complementary operation for plants with missing leaves. The PointConv model demonstrated the best performance, with an average accuracy, precision, and recall of 95.83, 95.83, and 95.88%, respectively, and a model loss of 0.01. This method employs spatial feature information to analyse different seedling categories more effectively than two-dimensional (2D) image classification and three-dimensional (3D) feature extraction methods. However, there is a paucity of studies applying 3D classification methods to predict useful eggplant seedling transplants. Consequently, this method has the potential to identify different eggplant seedling types with high accuracy. Furthermore, it enables the quality inspection of seedlings during agricultural production.
Evaluation of the Effects of Recent Weather Variations on Winter-Wheat Agronomic Characteristics, and Their Correlations in Jinju, Republic of Korea
Authors: Lee Jongtae , Moon Jinyoung , Kim Jinyoung , Yang Munhee , Kim Seonhui , Kim Boram , Ryu Eonjung , Hwang Yeon-Hyeon , Kim Young-Gwang , Kim Dea-Wook , Cho Seong-Woo
Published: September 2024

Wheat grain productivity is different from year to year because growing environments are highly seasonally variable as a result of climate change. This study analyzed the variation in the weather conditions in the 2010–2023 growing seasons and evaluated the crop developmental phase, yield-related components, and the correlations of the variables in the southern plain of South Korea, measuring agronomic traits, including the above-ground dry weight, young-panicle length, spike number per m2, number of grains per spike, thousand-grain weight, and grain yield. The number of days in the heading and ripening phase showed less differences than the other growth phases. The thousand-grain weight showed low variations over the fourteen years observed, unlike the number of grains per spike, the marketable grain yield, and the straw yield, with comparatively high variations. The grain yield was negatively correlated with the average air temperature during the winter dormancy phase (R = −0.687, p = 0.007) and precipitation (R = −0.726, p = 0.003), but showed positive associations with the number of days in the winter dormancy phase (R = 0.597, p = 0.024) and the number of grains per spike (R = 0.809, p = 0.000). In conclusion, longer winter dormancy and a longer tillering phase delay young-panicle development but increase the number of spikes and the number of grains per spike, resulting in a higher wheat grain yield in Southern Korean weather conditions.
CsNWD Encoding VPS62 Emerges as a Candidate Gene Conferring the Glabrous Phenotype in Cucumber
Authors: Zhou Yuelong , Xu Mingyuan , Zhou Yong , Hu Zhaoyang , Liu Shiqiang
Published: September 2024

The commercial value of cucumbers is influenced by the presence of spines or trichomes on their fruit, while the trichomes found on other parts of the plant are essential for protecting the cucumber against a range of biotic and abiotic stresses. In our research, we identified a glabrous nwd mutant from EMS-induced cucumber (Cucumis sativus L.) mutant lines. This mutant displays a consistent glabrous phenotype across all plant organs. Upon analysis, it was revealed that the nwd mutation is governed by a lone recessive nuclear gene. The nwd plants show a significant reduction in both their photosynthetic capacity and chlorophyll a content when compared to the wild-type. Furthermore, there are notable changes in the antioxidant enzyme activities, soluble protein levels, and malondialdehyde content of the nwd mutant. Genotyping and MutMap approaches identified the CsNWD gene, encoding a VPS protein, as a candidate responsible for the glabrous trait. Transcriptome analysis indicated that 15 trichome-related genes exhibit significant expression changes in the nwd mutant, implying that CsNWD may regulate trichome development through interactions with these genes. This study contributes to the understanding of VPS proteins in trichome initiation and the underlying mechanisms of trichome development.
Delayed Reproduction, Injury, and Regeneration of Testes in Out-of-Season Breeding of Largemouth Bass (Micropterus nigricans)
Authors: He Kuo , Yang Yi , Li Zhihong , Yan Haoxiao , Song Kaige , Liu Qiao , Zhao Liulan , Yang Song
Published: September 2024

Out-of-season breeding is an effective method for addressing seasonal shortages of fry in aquaculture species such as largemouth bass (LMB) for year-round production. Off-season breeding of LMB can be achieved by subjecting breeding LMB to prolonged low-temperature conditions; however, this can alter reproductive rhythms, affecting the quality of their sperm and leading to a decrease in reproductive efficiency. Therefore, it is crucial to investigate issues such as the damage to the testes and the related mechanisms caused by low-temperature stress during out-of-season breeding. In this experiment, we assessed the changes in the testes during this time in LMB by comparing reproductive rhythms, testicular histomorphology, ultrastructure, antioxidant capacity and apoptosis. We synthesized measurements of LMB from three identically treated cement ponds and fish exposed to water temperatures of 13–16 °C to assess the changes in the testes. The results showed that (1) out-of-season reproduction delayed sperm production and promoted sperm redevelopment in LMB, various hormone levels have changed over time (e.g., LH, FSH, and T). (2) The head plasma membrane of LMB spermatozoa was separated, and the middle mitochondria were swollen. (3) The expression levels of antioxidant enzymes (cat, sod, and gpx) were upregulated, and oxidative stress occurred in LMB. (4) The expression levels of apoptosis genes (e.g., bax, bcl2, and caspase3) were upregulated, and apoptosis occurred in LMB due to off-season breeding. Moreover, important genes of the mitochondrial apoptosis pathway (bid, CYT-C) were upregulated, indicating that spermatozoan apoptosis in LMB was probably achieved through the mitochondrial apoptosis pathway. These results suggest the delays, damage, and regeneration of LMB testes. Our findings provide new insights into the molecular mechanisms that trigger changes in sperm quality during out-of-season breeding in fish.
Eularian–Eularian Model for Agglomeration Behavior of Combusted Iron Particles
Authors: Fernando Warnakulasooriya Dinoja Sammani , Naser Jamal
Published: September 2024

Direct reduction of iron (DRI) technology in fluidized beds has been identified as a promising approach due to its environmental benefits over other methods. Nevertheless, the process of iron particle sintering in the DRI approach poses a significant obstacle to its advancement. The present work investigated the phenomenon of agglomeration in fine iron particles across various temperatures and with multiple sintering force models of different intensities of solid bridge force. The study utilized a simple but comprehensive and cost-effective CFD model developed using the Eularian–Eularian two-fluid model. The model was explicitly incorporated with user-defined subroutines for the solid phase, while the gas phase was modeled with AVL Fire advance simulation software. The solid bridge force between solid particles was modeled as the inter-particle cohesive force. The model was validated with the experimental results and results from another CFD-DEM model for the same experiment. High temperatures with increased sintering forces were observed to have the most impact on the iron particle agglomeration, while the gas’s superficial velocity had a minimal effect on it. The predictions of this model closely align with the CFD-DEM model results, providing sufficient reliability to implement this model on a large scale.
A Study on the Mechanical Resonance Frequency of a Piezo Element: Analysis of Resonance Characteristics and Frequency Estimation Using a Long Short-Term Memory Model
Authors: Moon Jeonghoon , Lim Sangkil , Kim Jinhong , Kang Geonil , Kim Beomhun
Published: September 2024

In an ultrasonic system, a piezoelectric transducer (PT) is a key component and contains a piezo element inside. In order to design and operate a system that uses a piezo element for its intended purpose, resonance analysis of the piezo element and an equivalent circuit analysis of the output stage of the ultrasonic system generator are required. Due to the characteristics of the equivalent circuit, a piezo element has multiple resonance points. Therefore, the system must be operated at the corresponding frequency by tracking the resonance frequency that suits the purpose of the system. In this study, the mechanical resonance frequency of the piezo element was analyzed and a method for operating the system at the corresponding frequency was studied. In order to operate a piezo element, a voltage-type inverter is used to apply a high-frequency AC (Alternating Current). Then, an LC filter is inserted into the output stage of the inverter, and the piezo element is finally located at the output stage of the LC filter. Therefore, when designing an LC filter, a design is required to optimize the performance of the piezo element. In this paper, we analyzed the resonance of a piezo element and the equivalent circuit of the generator output stage of an ultrasonic system for effective operation of an ultrasonic system. In addition, we proposed a method to estimate the characteristics of the entire mechanical resonance frequency range of a piezo element by using an LSTM (Long Short-Term Memory) model suitable for analyzing the nonlinear characteristics of a piezo element. The study on estimating the mechanical resonance frequency of a piezo element using an LSTM model was verified through MATLAB 2021b simulation and ultrasonic system experiments.