bluuebunny/crossref_metadata_2025 huggingface dataset:
Spodoptera frugiperda (Lepidoptera: Noctuidae) Life Table Comparisons and Gut Microbiome Analysis Reared on Corn Varieties
Authors: Jeon Jungwon , Rahman Md-Mafizur , Han Changhee , Shin Jiyeong , Sa Kyu Jin , Kim Juil
Published: April 2023

The fall armyworm (Spodoptera frugiperda, FAW) is an invasive migratory pest that has recently spread to Korea, damaging several corn cultivars with significant economic value. Comparisons of the growth stages of FAW were conducted based on the preferred feed. Therefore, we selected six maize cultivars, including three categories: (i) commercial waxy corn (mibaek 2-ho, heukjeom 2-ho, dreamoak); (ii) popcorn (oryun popcorn, oryun 2-ho); and (iii) processing corn (miheukchal). A significant effect was observed during the larvae period, pupal period, egg hatching ratio, and larvae weight, whereas the total survival period and adult period did not show significant variation among the tested corn cultivars. We identified variations in the FAW gut bacterial community that were dependent on the genotype of the corn maize feed. The identified phyla included Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. Among these genera, the most abundant bacterial genus was Enterococcus, followed by Ureibacillus. Enterococcus mundtii was the most abundant among the top 40 bacterial species. The intergenic PCR-based amplification and gene sequence of the colony isolates were also matched to the GenBank owing to the prevalence of E. mundtii. These results showed that the bacterial diversity and abundance of particular bacteria in the guts of FAWs were influenced by the six major maize corn cultivars.
Digital Application for Promoting Evidence-Based Children’s Oral Health to Control Early Childhood Caries: Randomized Control Trial on Parental Acceptance and Efficacy
Authors: Abdul Haq Jameela , Splieth Christian H. , Mourad Mhd Said , Vielhauer Annina , Abdulrahim Ruba , Khole Manasi R. , Santamaría Ruth M.
Published: April 2023

Background: Early childhood caries (ECC) remains a major global health problem. Various measures to prevent it have been implemented in the past, including those using digital applications. Aim: To evaluate the acceptance and efficacy of a digital application (FU-APP) based on evidence-based caries control recommendations for parents of children aged 6–72 months. Methods: Part 1, prospective questionnaire-based survey to test FU-APP (usage, acceptance, content information, usefulness, and satisfaction) filled out by parents (n = 22); Part 2, two-armed (test n = 20; control n = 23) care-based, randomized controlled trial, where the test arm received instructions verbally and via FU-APP, and the control arm received them only verbally. At baseline and follow-up (4 weeks), intraoral clinical indices (plaque index-API and caries-dmft) were recorded. Results: FU-APP was considered by parents to be a suitable tool for gaining knowledge about oral health practices for their children (all criteria >86%). No differences in the dmft levels were expected. However, API was significantly better at the follow-up in the test-arm (p = 0.01), with no differences in the control-arm (p = 0.72). Conclusion: A digital application can serve as an innovative tool to promote evidence-based oral hygiene recommendations among parents of children to control ECC. Its long-term usability and functionality should be tested.
Changes in Revolving-Door Mental Health Hospitalizations during the COVID-19 Pandemic: A 5-Year Chart Review Study
Authors: Napoli Giovanni , Garzitto Marco , Magliulo Vincenzo , Carnemolla Rossana , Anzallo Calogero , Balestrieri Matteo , Colizzi Marco
Published: April 2023

This study assessed changes in revolving-door (RD) mental health hospitalizations during the COVID-19 pandemic. A 5-year retrospective hospital chart review was performed, collecting revolving-door hospitalization, sociodemographic, and clinical data. Out of 1036 patients, 5.69% had RD hospitalizations, which accounted for 10.38% of all recorded hospitalizations. Further, a higher number of RD hospitalizations occurred following the pandemic outbreak, which is unlikely to have been a result of the confounding effect of trimester and month of hospitalization. Finally, several sociodemographic and clinical characteristics recurred more frequently in the context of RD hospitalizations, such as being younger, being compulsorily admitted, being an absconding patient, and being referred by a public service. Certain diagnostic categories occurred more frequently among RD hospitalizations, including psychotic, personality, and substance use disorders, as well as intellectual disability. Patients with specific characteristics are more likely to incur in RD hospitalizations, requiring the implementation of supportive treatment plans, especially following the pandemic outbreak.
Chronic Limb-Threatening Ischemia and the Need for Revascularization
Authors: Berchiolli Raffaella , Bertagna Giulia , Adami Daniele , Canovaro Francesco , Torri Lorenzo , Troisi Nicola
Published: April 2023

Background: Patients presenting with critical limb-threatening ischemia (CLTI) have been increasing in number over the years. They represent a high-risk population, especially in terms of major amputation and mortality. Despite multiple guidelines concerning their management, it continues to be challenging. Decision-making between surgical and endovascular procedures should be well established, but there is still a lack of consensus concerning the best treatment strategy. The aim of this manuscript is to offer an overview of the contemporary management of CLTI patients, with a focus on the concept that evidence-based revascularization (EBR) could help surgeons to provide more appropriate treatment, avoiding improper procedures, as well as too-high-risk ones. Methods: We performed a search on MEDLINE, Embase, and Scopus from 1 January 1995 to 31 December 2022 and reviewed Global and ESVS Guidelines. A total of 150 articles were screened, but only those of high quality were considered and included in a narrative synthesis. Results: Global Vascular Guidelines have improved and standardized the way to classify and manage CLTI patients with evidence-based revascularization (EBR). Nevertheless, considering that not all patients are suitable for revascularization, a key strategy could be to stratify unfit patients by considering both clinical and non-clinical risk factors, in accordance with the concept of individual residual risk for every patient. The recent BEST-CLI trial established the superiority of autologous vein bypass graft over endovascular therapy for the revascularization of CLTI patients. However, no-option CLTI patients still represent a critical issue. Conclusions: The surgeon’s experience and skillfulness are the cornerstones of treatment and of a multidisciplinary approach. The recent BEST-CLI trial established that open surgical peripheral vascular surgery could guarantee better outcomes than the less invasive endovascular approach.
Τhe Impact of Pre-Transplant Kidney Biopsy on the Evaluation of Prospective Living Kidney Donors
Authors: Marinaki Smaragdi , Vallianou Kalliopi , Darema Maria , Mantios Evangelos , Kapsia Eleni , Melexopoulou Christina , Filiopoulos Vassilis , Liapis George , Boletis Ioannis N.
Published: April 2023

Living kidney donation contributes to increasing the donor pool. Since safety and excellent outcomes of living kidney donors (LKD) are essential, renal biopsy must be part of the pre-transplant evaluation in donors with isolated urine abnormalities or other risk factors. We retrospectively collected data on potential living donors evaluated in the pre-transplant outpatient clinic of Laiko General Hospital of Athens between 2007 and 2022, who underwent a pre-transplant biopsy. Biopsy indications included microscopic hematuria, borderline proteinuria and comorbidities suggestive of chronicity. Those with glomerular diseases or chronic lesions were excluded from donation. We identified 59 potential living donors who underwent renal biopsy. Of these, 10 (16.9%) were male. Median age was 58 (IQR 51–63) years, while 23 (39%) were older than 60 years. 49 out of 59 (83%) had glomerular hematuria, 10 (16.7%) had proteinuria (150–300 mg/d). Out of the 59 donors, 21 (35.6%) were hypertensive, three (5.1%) had impaired glucose tolerance and seven (11.9%) had a BMI > 30 kg/m2. A total of 32 (54.2%) potential donors were accepted for donation. Eight (13.6%) had IgA nephropathy, 10 (16.9%) TBMD and nine (15.3%) had increased chronicity including secondary FSGS. When compared with a control group of donors who did not need a pre-transplant biopsy, those 32 who donated were more frequently hypertensive (p = 0.003), but had similar eGFR [61.3 (±10.4) vs. 61.9 (±13.8), p = 0.866] after a follow-up of 79 (36–114) months. Renal biopsy is a useful tool in the evaluation of prospective LKD. Thorough assessment of donors with isolated urine abnormalities and marginal donors is critical to ensure good post-donation outcomes.
Surrogates of Muscle Mass on Cardiac MRI Correlate with Exercise Capacity in Patients with Fontan Circulation
Authors: Smith Kevin L. , Gordon Emile B. , Gunsaulus Megan E. , Christopher Adam , Olivieri Laura J. , Tadros Sameh S. , Harris Tyler , Saraf Anita P. , Kreutzer Jacqueline , Feingold Brian , Alsaied Tarek
Published: April 2023

Background: Sarcopenia is an increasingly recognized marker of frailty in cardiac patients. Patients with a history of congenital heart disease and Fontan procedure have a higher risk of developing progressive muscle wasting. Our objective was to determine if we could use routine cardiac MRI (CMR) for the surveillance of muscle wasting. Methods: A retrospective study of all Fontan patients (n = 75) was conducted at our institution, with CMR performed from 2010 to 2022 and exercise stress testing performed within 12 months (4.3 ± 4.2 months). The skeletal muscle area (SMA) for the posterior paraspinal and anterior thoracic muscles were traced and indexed for body surface area (BSA). Patients were stratified by percentile into the upper and lower quartiles, and the two groups were compared. Multivariable regression was performed to control for sex and age. Results: There was a significant positive association of both anterior (r = 0.34, p = 0.039) and paraspinal (r = 0.43, p = 0.007) SMA to peak VO2. Similarly, paraspinal but not anterior SMA was negatively associated with the VE/VCO2 (r = –0.45, p = 0.006). The upper quartile group had significantly more males (18/19 vs. 8/20; p = 0.0003) and demonstrated a significantly higher peak VO2 (32.2 ± 8.5 vs. 23.8 ± 4.7, p = 0.009), a higher peak RER (1.2 ± 0.1 vs. 1.1 ± 0.04, p = 0.007), and a significantly lower VE/VCO2 (32.9 ± 3.6 vs. 40.2 ± 6.2, p = 0.006) compared to the lowest quartile. The association of SMA to VO2 peak and VE/VCO2 was redemonstrated after controlling for sex and age. Conclusion: Thoracic skeletal muscle area may be an effective surrogate of muscle mass and is correlated to several measures of cardiorespiratory fitness post-Fontan. CMR would be an effective tool for the surveillance of sarcopenia in post-Fontan patients given its accessibility and routine use in these patients.
Setting the Tibial Component Rotation Based on Femoral Landmarks Allows Congruent Knee Kinematics in Robotic-Assisted Medial Unicompartmental Knee Replacement
Authors: Innocenti Matteo , Zanna Luigi , Akkaya Mustafa , Huber Kim , Christen Bernhard , Calliess Tilman
Published: April 2023

The accurate positioning of the prosthetic components is essential for achieving successful results in medial unicompartmental knee arthroplasty (mUKA). The tibial component rotation in image-based robotic-assisted UKA is usually based on tibial bony landmarks matched to the pre-operative CT model. The study aimed to evaluate whether setting the tibial rotation on femoral CT-based landmarks allows congruent knee kinematics. We retrospectively analyzed data from 210 consecutive image-based robotic-assisted mUKA cases. In every case, we set the tibia rotation landmark parallel to the posterior condylar axis and centered it on the trochlea groove defined on the preoperative CT scan. The implant positioning was primarily set parallel to this rotation landmark and then adjusted based on tibial sizes avoiding component over- or under-hang. During surgery, we recorded the knee kinematics under valgus stress to reduce the arthritic deformity. A femoral-tibial contact point was recorded over the entire range of motion and displayed as a tracking profile on the tibia implant. The femoro-tibial tracking angle (FTTA) was then calculated based on a tangent line to the femoro-tibial tracking-points and the difference to the femur-based rotation landmark. In 48% of the cases, we could position the tibia component exactly to the femoral rotation landmark, whereas in 52% of cases, minimal adjustments were made to avoid component’s under- or over-hang. The mean tibia component rotation (TRA) with reference to our femur-based landmark was +0.24° (SD ± 2.9°). The femur-based tibia rotation landmark showed a high correspondence to the FTTA with 60% of the cases having less than 1° of deviation. Mean FTTA was +0.7° (SD ± 2.2°). The mean difference between the absolute value of the TRA and the FTTA (|TRA| − |FTTA|) was −0.18° (SD ± 2°). Setting the tibial component rotation based on CT scan femoral landmarks and not on tibial anatomical landmarks is a reliable method to obtain congruent knee kinematics during image-based robotic-assisted medial UKA with less the 2° deviations on average.
Research on the Changes in Cultural Landscape of Tourist-Type Traditional Chinese Villages from the Perspective of Cultural Memory: Taking Anzhen Village in Chongqing as an Example
Authors: Yang Qi
Published: April 2023

With the development of rural tourism, rural culture in China has suffered from development problems. Existing studies focus more on how to protect rural culture, ignoring the dynamic changes in rural culture. Therefore, cultural memory offers an important perspective to review the dynamic process of cultural changes. This paper uses the concept of cultural landscape to explore the changes in rural culture from the perspective of cultural memory. The research methods of historical data analysis and field investigation are used. The change process of the cultural landscape in Anzhen Village is explored. Firstly, the clan-style group life in the traditional human–land relationship is the germination of cultural memory. Secondly, typical Chongqing residential mansions become ways of spatial expression of the local cultural landscape. Thirdly, the group interpretation of the settlement landscape becomes the continuation of local memory. Fourthly, the migration of living settlements in the dual influence of modern transportation and administrative divisions is the deconstruction of local memory. Fifthly, the touristic reconstruction of the traditional cultural landscape is summarized as memory reconstruction. In addition, the factors affecting the changes in the cultural landscape are analyzed from both internal and external aspects. The discussion on the dynamic changes in the cultural landscape provides a theoretical and practical basis for the analysis, protection, and inheritance of rural culture.
Satellite-Based Carbon Estimation in Scotland: AGB and SOC
Authors: Chan Chun Ki , Gomez Carla Arus , Kothikar Anish , Baiz-Villafranca P. M.
Published: April 2023

The majority of state-of-the-art research employs remote sensing on AGB (Above Ground Biomass) and SOC (Soil Organic Carbon) separately, although some studies indicate a positive correlation between the two. We intend to combine the two domains in our research to improve state-of-the-art total carbon estimation. We begin by establishing a baseline model in our study area in Scotland, using state-of-the-art methodologies in the SOC and AGB domains. The effects of feature engineering techniques such as variance inflation factor and feature selection on machine learning models are then investigated. This is extended by combining predictor variables from the two domains. Finally, we leverage the possible correlation between AGB and SOC to establish a relationship between the two and propose novel models in an attempt to outperform the state-of-the-art results. We compared three machine learning techniques, boosted regression tree, random forest, and xgboost. These techniques have been demonstrated to be the most effective in both domains. This research makes three contributions: (i) Including Digital Elevation Map (DEM) as a predictor variable in the AGB model improves the model result by 13.5 % on average across the three machine learning techniques experimented, implying that DEM should be considered for AGB estimation as well, despite the fact that it has previously been used exclusively for SOC estimation. (ii) Using SOC and SOC Density improves the prediction of the AGB model by a significant 14.2% on average compared to the state-of-the-art baseline (When comparing the R2 value across all three modeling techniques in Model B and Model H, there is an increase from 0.5016 to 0.5604 for BRT, 0.4958 to 0.5925 for RF and 0.5161 to 0.5750 for XGB), which strengthens our experiment results and suggests a future research direction of combining AGB and SOC as a joint study domain. (iii) Including AGB as a predictor variable for SOC improves model performance for Random Forest, but reduced performance for Boosted Regression tree and XG Boost, indicating that the results are specific to ML models and more research is required on the feature space and modeling techniques. Additionally, we propose a method for estimating total carbon using data from Sentinel 1, Sentinel 2, Landsat 8, Digital Elevation, and the Forest Inventory.
Effects of Turmeric Extract on Age-Related Skeletal Muscle Atrophy in Senescence-Accelerated Mice
Authors: Lyu Weida , Kousaka Marika , Jia Huijuan , Kato Hisanori
Published: April 2023

Muscle atrophy is one of the main causes of sarcopenia—the age-related loss of skeletal muscle. In this study, we investigated the effect of turmeric (Curcuma longa) extract (TE) supplementation on age-related muscle atrophy in a senescence-accelerated mouse model and explored the underlying mechanisms. Twenty-six-week-old male, senescence-accelerated mouse resistant (SAMR) mice received the AIN-93G basal diet, while twenty-six-week-old male, senescence-accelerated mouse prone 8 (SAMP8) mice received the AIN-93G basal diet or a 2% TE powder-supplemented diet for ten weeks. Our findings revealed that TE supplementation showed certain effects on ameliorating the decrease in body weight, tibialis anterior weight, and mesenteric fat tissue weight in SAMP8 mice. TE improved gene expression in the glucocorticoid receptor-FoxO signaling pathway in skeletal muscle, including redd1, klf15, foxo1, murf1, and mafbx. Furthermore, TE might have the certain potential on improving the dynamic balance between anabolic and catabolic processes by inhibiting the binding of glucocorticoid receptor or FoxO1 to the glucocorticoid response element or FoxO-binding element in the MuRF1 promoter in skeletal muscle, thereby promoting muscle mass and strength, and preventing muscle atrophy and sarcopenia prevention. Moreover, TE may have reduced mitochondrial damage and maintained cell growth and division by downregulating the mRNA expression of the genes mfn2 and tsc2. Thus, the results indicated TE’s potential for preventing age-related muscle atrophy and sarcopenia.
Insight into Elderly ALS Patients in the Emilia Romagna Region: Epidemiological and Clinical Features of Late-Onset ALS in a Prospective, Population-Based Study
Authors: Gianferrari Giulia , Martinelli Ilaria , Simonini Cecilia , Zucchi Elisabetta , Fini Nicola , Caputo Maria , Ghezzi Andrea , Gessani Annalisa , Canali Elena , Casmiro Mario , De Massis Patrizia , Curro’ Dossi Marco , De Pasqua Silvia , Liguori Rocco , Longoni Marco , Medici Doriana , Morresi Simonetta , Patuelli Alberto , Pugliatti Maura , Santangelo Mario , Sette Elisabetta , Stragliati Filippo , Terlizzi Emilio , Vacchiano Veria , Zinno Lucia , Ferro Salvatore , Amedei Amedeo , Filippini Tommaso , Vinceti Marco ,   ERRALS GROUP, Mandrioli Jessica
Published: April 2023

Few studies have focused on elderly (>80 years) amyotrophic lateral sclerosis (ALS) patients, who represent a fragile subgroup generally not included in clinical trials and often neglected because they are more difficult to diagnose and manage. We analyzed the clinical and genetic features of very late-onset ALS patients through a prospective, population-based study in the Emilia Romagna Region of Italy. From 2009 to 2019, 222 (13.76%) out of 1613 patients in incident cases were over 80 years old at diagnosis, with a female predominance (F:M = 1.18). Elderly ALS patients represented 12.02% of patients before 2015 and 15.91% from 2015 onwards (p = 0.024). This group presented with bulbar onset in 38.29% of cases and had worse clinical conditions at diagnosis compared to younger patients, with a lower average BMI (23.12 vs. 24.57 Kg/m2), a higher progression rate (1.43 vs. 0.95 points/month), and a shorter length of survival (a median of 20.77 vs. 36 months). For this subgroup, genetic analyses have seldom been carried out (25% vs. 39.11%) and are generally negative. Finally, elderly patients underwent less frequent nutritional- and respiratory-supporting procedures, and multidisciplinary teams were less involved at follow-up, except for specialist palliative care. The genotypic and phenotypic features of elderly ALS patients could help identify the different environmental and genetic risk factors that determine the age at which disease onset occurs. Since multidisciplinary management can improve a patient’s prognosis, it should be more extensively applied to this fragile group of patients.
Physico-Mechanical Performances of Mortars Prepared with Sorted Earthquake Rubble: The Role of CDW Type and Contained Crystalline Phases
Authors: Galderisi Antonio , Bravo Miguel , Iezzi Gianluca , Cruciani Giuseppe , Paris Eleonora , Brito Jorge de
Published: April 2023

Construction and demolition waste (CDW) from earthquake rubbles was used here as recycled aggregates (RA) in cementitious binders. The materials were sorted in six groups: concrete (CO), natural stone (NS), tile (TI), brick (BR), perforated brick (PF) and roof tile (RT). The abundance (wt.%) of crystalline phases in each RA type was determined by X-ray Powder Diffraction (XRPD). Each group of RAs was used alone (100 wt.% of RA) and mixed with quartz-rich virgin aggregates (VA) to prepare 13 types of mortars (12 specimens per type): one reference mortar (RM) with only VA, six recycled aggregate mortars (RAM) and six recycled-plus-virgin aggregate mortars (RVAM). The physical and mechanical properties of aggregates and mortars reflect the type and abundance of crystalline phases in each CDW group. Recycled mortars rich in concrete, natural stones and tiles have better mechanical performance than mortars prepared with recycled bricks, perforated bricks and roof tiles. For each RA, RVAMs have superior mechanical characteristics than the corresponding RAM. Since the type and amount of phases contained in recycled aggregates strongly control the mechanical performance of new construction materials, they should be routinely quantified as reported here, in addition to other physical features (water absorption, density, etc.). The separation of heterogeneous CDW into homogeneous RA groups is necessary for the production of new construction materials with stable and predictable performances to ensure CDW recycling, especially in areas hit by major adverse events, where large amounts of still valuable materials could be used for reconstruction processes.
Photoluminescence and Photocatalytic Properties of MWNTs Decorated with Fe-Doped ZnO Nanoparticles
Authors: Popa Adriana , Stefan Maria , Macavei Sergiu , Muresan Laura Elena , Leostean Cristian , Floare-Avram Cornelia Veronica , Toloman Dana
Published: April 2023

The present work reports the photoluminescence (PL) and photocatalytic properties of multi-walled carbon nanotubes (MWCNTs) decorated with Fe-doped ZnO nanoparticles. MWCNT:ZnO-Fe nanocomposite samples with weight ratios of 1:3, 1:5 and 1:10 were prepared using a facile synthesis method. The obtained crystalline phases were evidenced by X-ray diffraction (XRD). X-ray Photoelectron spectroscopy (XPS) revealed the presence of both 2+ and 3+ valence states of Fe ions in a ratio of approximately 0.5. The electron paramagnetic resonance EPR spectroscopy sustained the presence of Fe3+ ions in the ZnO lattice and evidenced oxygen vacancies. Transmission electron microscopy (TEM) images showed the attachment and distribution of Fe-doped ZnO nanoparticles along the nanotubes with a star-like shape. All of the samples exhibited absorption in the UV region, and the absorption edge was shifted toward a higher wavelength after the addition of MWCNT component. The photoluminescence emission spectra showed peaks in the UV and visible region. Visible emissions are a result of the presence of defects or impurity states in the material. All of the samples showed photocatalytic activity against the Rhodamine B (RhB) synthetic solution under UV irradiation. The best performance was obtained using the MWCNT:ZnO-Fe(1:5) nanocomposite samples, which exhibited a 96% degradation efficiency. The mechanism of photocatalytic activity was explained based on the reactive oxygen species generated by the nanocomposites under UV irradiation in correlation with the structural and optical information obtained in this study.
Semiconductor Characterization by Terahertz Excitation Spectroscopy
Authors: Krotkus Arūnas , Nevinskas Ignas , Norkus Ričardas
Published: April 2023

Surfaces of semiconducting materials excited by femtosecond laser pulses emit electromagnetic waves in the terahertz (THz) frequency range, which by definition is the 0.1–10 THz region. The nature of terahertz radiation pulses is, in the majority of cases, explained by the appearance of ultrafast photocurrents. THz pulse duration is comparable with the photocarrier momentum relaxation time, thus such hot-carrier effects as the velocity overshoot, ballistic carrier motion, and optical carrier alignment must be taken into consideration when explaining experimental observations of terahertz emission. Novel commercially available tools such as optical parametric amplifiers that are capable of generating femtosecond optical pulses within a wide spectral range allow performing new unique experiments. By exciting semiconductor surfaces with various photon energies, it is possible to look into the ultrafast processes taking place at different electron energy levels of the investigated materials. The experimental technique known as the THz excitation spectroscopy (TES) can be used as a contactless method to study the band structure and investigate the ultrafast processes of various technologically important materials. A recent decade of investigations with the THz excitation spectroscopy method is reviewed in this article. TES experiments performed on the common bulk A3B5 compounds such as the wide-gap GaAs, and narrow-gap InAs and InSb, as well as Ge, Te, GaSe and other bulk semiconductors are reviewed. Finally, the results obtained by this non-contact technique on low-dimensional materials such as ultrathin mono-elemental Bi films, InAs, InGaAs, and GaAs nanowires are also presented.
Synthesis of Coal-Fly-Ash-Based Ordered Mesoporous Materials and Their Adsorption Application
Authors: Tan Miaomiao , Pan Dahai , Chen Shuwei , Yan Xiaoliang , Han Lina , Li Ruifeng , Wang Jiancheng
Published: April 2023

A feasible approach was developed for the synthesis of ordered mesoporous SBA-15-type materials using coal fly ash (CFA) as raw material. In the proposed approach, CFA was, firstly, activated by subcritical water with the addition of NaOH, which allowed an efficient extraction of silicon species from CFA under strong acidic conditions at near room temperature. Subsequently, in the synthesis system, using silicon extraction solution as the silicon precursor, the introduction of anhydrous ethanol as a co-solvent effectively inhibited the polymerization of silanol species and promoted their collaborative self-assembly with surfactant molecules by enhancing the hydrogen bond interactions. The resultant SBA-15 material had a high purity, high specific surface area (1014 m2/g) and pore volume (1.08 cm3/g), and a highly ordered mesostructure, and, therefore, exhibited an excellent removal efficiency (90.5%) and adsorption capacity (160.8 mg/g) for methylene blue (MB) from simulated wastewater. Additionally, the generation of surface acid sites from the homogenous incorporation of Al atoms onto the mesoporous walls of SBA-15 combined with the perfect retention of the ordered mesostructure endowed the obtained Al-SBA-15 material with a further boost in the removal performance of MB. The MB removal efficiency can reach ~100%, along with a maximum adsorption capacity of 190.1 mg/g.
The Parameter Identification of Physical-Based Constitutive Model by Inverse Analysis Method for Application in Near-Net Shape Forging of Aluminum Wheels
Authors: Chen Lingling , Yuan Chaolong , Wu Rendong , Jiao Wei , Jiang Haishun , Zhou Xingyou
Published: April 2023

A reliable constitutive model is a prerequisite to simulate a new complex forming technique, which is represented by the near-net shape forging process of aluminum wheels in this study. The aim of the present work was to identify the physical-based constitutive model parameters of Al-Zn-Mg alloy via the inverse analysis method based on experimental data and numerical analysis: the stress–strain curves at different temperatures and strain rates were obtained based on hot compression tests. On the basis of the shape of the compressed specimens and experimental force–displacement data, the friction coefficients and the optimized physical-based constitutive model were determined by using two-times inverse analysis techniques. Results showed that the global average error between the predicted and experimental force–displacement curves was only 3.8%. Then, thermo-mechanical finite element models were built in the Deform-3D software to simulate the two-stage forging processes of the near-net shape forging of aluminum alloy wheels, and the results showed that the predicted load–stroke curves were in good agreement with the experimental ones in all forging stages, which verified the prediction accuracy of the optimized physical-based constitutive model. In addition, the identification of the physical-based constitutive model parameters by the inverse analysis method provides a theoretical basis for formulating and optimizing the near-net shape forging process parameters of aluminum alloy wheels.
Research on the Electric-Pulse-Assisted Turning Behavior of TC27 Alloy
Authors: Guan Huashen , Zhong Yanzhen , Zou Wei , Sun Pengfei , Zhai Jianshuo , Qu Shengguan
Published: April 2023

As a difficult-to-machine material widely used in the aerospace industry, the high-quality and efficient cutting of titanium alloys has been a hot issue in the field of machining. This work performed electric-pulse-assisted turning (EPT) and conventional turning (CT) for Ti5Al4Mo6V2Nb1Fe (TC27) alloy with two kinds of cutters. The results showed that electric pulses significantly improved the machining performance and surface finish quality. With the help of the electric pulse, the cutting force of TiAlN-coated cemented carbide insert (CCI) and uncoated carbide insert (UCI) tools during turning was reduced by more than 20%, and the surface roughness decreased by about 30% with a root mean square of the current density of 0.9 A/mm2, because the larger current led to a more obvious electro-plasticity effect. Compared with CT, the surface microhardness of the EPT samples processed with different current densities decreased; this is because the hardened surface was softened by both thermal and athermal effects under the continuous pulse current. However, the microhardness of the EPT samples was still higher than the matrix. At the same time, EPT reduces the wear of the cutting tool, thus helping to extend the tool’s service life. Finally, the electric pulse parameters recommended to assist turning are as follows: an electro-pulsing frequency of 600 Hz, a root mean square of the current density in the cross-section of 0.57 A/mm2, and a lasting time of the current per circle of 100 μs.
Unveiling the Alloying-Processing-Microstructure Correlations in High-Formability Sheet Magnesium Alloys
Authors: Yang Jiyong , Shi Renhai , Luo Alan A.
Published: April 2023

Designing magnesium sheet alloys for room temperature (RT) forming is a challenge due to the limited deformation modes offered by the hexagonal close-packed crystal structure of magnesium. To overcome this challenge for lightweight applications, critical understanding of alloying-processing–microstructure relationship in magnesium alloys is needed. In this work, machine learning (ML) algorithms have been used to fundamentally understand the alloying-processing–microstructure correlations for RT formability in magnesium alloys. Three databases built from 135 data collected from the literature were trained using 10 commonly used machine learning models. The accuracy of the model is obviously improved with the increase in the number of features. The ML results were analyzed using advanced SHapley Additive exPlanations (SHAP) technique, and the formability descriptors are ranked as follows: (1) microstructure: texture intensity > grain size; (2) annealing processing: time > temperature; and (3) alloying elements: Ca > Zn > Al > Mn > Gd > Ce > Y > Ag > Zr > Si > Sc > Li > Cu > Nd. Overall, the texture intensity, annealing time and alloying Ca are the most important factors which can be used as a guide for high-formability sheet magnesium alloy design.
Therapeutic Potentials of Reducing Liver Fat in Non-Alcoholic Fatty Liver Disease: Close Association with Type 2 Diabetes
Authors: Tsamos Georgios , Vasdeki Dimitra , Koufakis Theocharis , Michou Vassiliki , Makedou Kali , Tzimagiorgis Georgios
Published: April 2023

Nonalcoholic fatty liver disease (NAFLD), the most widespread chronic liver disease worldwide, confers a significant burden on health systems and leads to increased mortality and morbidity through several extrahepatic complications. NAFLD comprises a broad spectrum of liver-related disorders, including steatosis, cirrhosis, and hepatocellular carcinoma. It affects almost 30% of adults in the general population and up to 70% of people with type 2 diabetes (T2DM), sharing common pathogenetic pathways with the latter. In addition, NAFLD is closely related to obesity, which acts in synergy with other predisposing conditions, including alcohol consumption, provoking progressive and insidious liver damage. Among the most potent risk factors for accelerating the progression of NAFLD to fibrosis or cirrhosis, diabetes stands out. Despite the rapid rise in NAFLD rates, identifying the optimal treatment remains a challenge. Interestingly, NAFLD amelioration or remission appears to be associated with a lower risk of T2DM, indicating that liver-centric therapies could reduce the risk of developing T2DM and vice versa. Consequently, assessing NAFLD requires a multidisciplinary approach to identify and manage this multisystemic clinical entity early. With the continuously emerging new evidence, innovative therapeutic strategies are being developed for the treatment of NAFLD, prioritizing a combination of lifestyle changes and glucose-lowering medications. Based on recent evidence, this review scrutinizes all practical and sustainable interventions to achieve a resolution of NAFLD through a multimodal approach.
Persistent Lipid Accumulation Leads to Persistent Exacerbation of Endoplasmic Reticulum Stress and Inflammation in Progressive NASH via the IRE1α/TRAF2 Complex
Authors: Lei Na , Song Hongfei , Zeng Ling , Ji Shaoxiu , Meng Xiangbo , Zhu Xiuying , Li Xiuyan , Feng Quansheng , Liu Jibin , Mu Jie
Published: April 2023

Non-alcoholic steatohepatitis (NASH) is a metabolic disorder that often leads to other severe liver diseases, yet treatment options are limited. Endoplasmic reticulum (ER) stress is an important pathogenetic mechanism of NASH and plays a key role in tandem steatosis as well as liver inflammation. This study aims to develop a progressive NASH model through sustained lipid accumulation and to elucidate its molecular mechanism through IRE1α/TRAF2 complex. Male SD rats were fed a high-fat diet (HFD) for 4, 8, and 12 weeks to induce progressive NASH. MRNA sequencing and PPI analysis were used to screen core genes. Transmission electron microscopy, immunofluorescence staining, ELISA, qRT-PCR, and Western blotting were used at each time point to compare differences between each index of progressive NASH at 4, 8, and 12 weeks. Sustained lipid accumulation led to structural disruption of the ER, a reduction in ER number, and an increase of lipid droplet aggregation in hepatocytes. Persistent lipid accumulation led to a persistent increase in mRNA and protein expression of the IRE1α/TRAF2 complex, IKK/IκB/NF-κB signaling pathway and ASK1/JNK1 signaling pathway, and TNF-α, IL-1β, and IL-6 also continued to increase. Persistent lipid accumulation led to a persistent exacerbation of ER stress and inflammation in progressive NASH via the IRE1α/TRAF2 complex.
Physicochemical and Antibacterial Evaluation of TiO2/CNT Mesoporous Nanomaterials Prepared by High-Pressure Hydrothermal Sol–Gel Method under an Ultrasonic Composite Environment
Authors: Lai Huansheng , Zhao Zilong , Yu Wenhe , Lin Yuan , Feng Zhiyuan
Published: April 2023

TiO2 has attracted significant research interest, principally due to its nontoxicity, high stability, and abundance. Carbon-doped TiO2 can improve light absorption efficiency. In order to prepare high-efficiency photocatalysts, carbon-doped composites were prepared by hydrothermal reaction in a high-pressure reactor, and then TiO2/CNT mesoporous composites were prepared by the sol–gel method in an ultrasonic environment. Characterized by SEM and TEM, the composite materials contained TiO2 nanoparticles as well as CNT. After phase analysis, it was the anatase-doped phase. The following infrared light absorption performance and Escherichia coli bactericidal performance tests showed that it had better infrared and visible light absorption performance than pure TiO2. The TiO2/CNT mesoporous nanomaterials synthesized in this work are possible for clean industrial productions.
Isoxazolyl-Derived 1,4-Dihydroazolo[5,1-c][1,2,4]Triazines: Synthesis and Photochemical Properties
Authors: Sadchikova Elena V. , Safronov Nikita E. , Beliaev Nikolai A. , Nenajdenko Valentine G. , Belskaya Nataliya P.
Published: April 2023

New fluorescent dyes containing an assembled 1,4-dihydroazolo[5,1-c][1,2,4]triazine (DAT) core and an isoxazole ring were synthesized through a reaction between diazopyrazole or diazoimidazoles and isoxazolyl-derived enamines in mild conditions. The photophysical characteristics (maxima absorption and emission, Stokes shifts, fluorescent quantum yields, and fluorescence lifetimes) of the new fluorophores were obtained. The prepared DATs demonstrated emission maxima ranging within 433–487 nm, quantum yields within 6.1–33.3%, and a large Stokes shift. The photophysical characteristics of representative DAT examples were studied in ten different solvents. Specific (hydrogen bonds) and non-specific (dipole–dipole) intermolecular and intramolecular interactions were analyzed using XRD data and spectral experiments. Solvatochromism was analyzed using Lippert–Mataga and Dimroth–Reichardt plots, revealing the relationship between the DAT structure and the nature of solute–solvent interactions. The significant advantages of DATs are the fluorescence of their powders (QY up to 98.7%). DAT-NMe2 10 expressed bright aggregation-induced emission (AIE) behavior in DMSO and THF as the water content increased. The numerous possible variations of the structures of the heterocycles included in the DATs, as well as substituents, create excellent prospects for adjusting their photophysical and physicochemical properties.
Chemical Characterization and Several Bioactivities of Cladanthus mixtus from Morocco
Authors: El Mihyaoui Amina , Erbiai El Hadi , Charfi Saoulajan , Pinto Eugénia , Castillo María Emilia Candela , Hernández-Ruiz Josefa , Cano Antonio , Badoc Alain , Lamarti Ahmed , Esteves da Silva Joaquim C. G. , Arnao Marino B.
Published: April 2023

The purpose of this work was to investigate, for the first time to our knowledge, the chemical composition and bioactivity of methanolic extracts (roots, stems, leaves, and flowers) from Cladanthus mixtus (L.) Chevall. that grows wild in northern Morocco (the Tangier-Tetouan-Al Hoceima region). The phenolic and flavonoid contents were determined by spectrophotometer methods, and the composition of derivatized methanolic extracts from C. mixtus using N-O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) was analyzed by gas chromatography–mass spectrometry (GC-MS). The antioxidant activity was carried out by applying the 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and DPPH (2,2-diphenyl-1-picrylhydrazyl) tests. The micro-dilution technique was chosen to investigate the antimicrobial activity of methanolic extracts against two bacterial strains and three fungal species. The results showed that the values of total phenolic and flavonoid contents were found to be higher in flower extracts (30.55 ± 0.85 mg of gallic acid equivalents (GAE)/g of dried weight (DW) and 26.00 ±1.34 mg of quercetin equivalents (QE)/g DW, respectively). Other groups of chemical compounds were revealed by GC-MS, such as carbohydrates (27.25–64.87%), fatty acids (1.58–9.08%), organic acids (11.81–18.82%), and amino acids (1.26–7.10%). Root and flower methanolic extracts showed the highest antioxidant activity using ABTS (39.49 mg of Trolox equivalents (TE)/g DW) and DPPH (36.23 mg TE/g DW), respectively. A positive correlation between antioxidant activity and polyphenol and flavonoid amounts was found. Antibacterial tests showed that the best activity was presented by the leaf extract against Staphylococcus aureus (minimum inhibitory concentration (MIC) = minimum bactericidal concentration (MBC) = 20 mg/mL) and Escherichia coli (MIC of 30 mg/mL and MBC of 35 mg/mL). S. aureus was more sensitive to the extracts compared to E. coli. All extracts showed antifungal activity against Trichophyton rubrum, with the best efficacy reported by the flower and leaf extracts (MIC = 1.25 mg/mL and minimum fungicidal concentration (MFC) = 2.5 mg/mL). In general, extracts of C. mixtus appeared less effective against Candida albicans and Aspergillus fumigatus.
Selenium in Peptide Chemistry
Authors: Pehlivan Özge , Waliczek Mateusz , Kijewska Monika , Stefanowicz Piotr
Published: April 2023

In recent years, researchers have been exploring the potential of incorporating selenium into peptides, as this element possesses unique properties that can enhance the reactivity of these compounds. Selenium is a non-metallic element that has a similar electronic configuration to sulfur. However, due to its larger atomic size and lower electronegativity, it is more nucleophilic than sulfur. This property makes selenium more reactive toward electrophiles. One of the most significant differences between selenium and sulfur is the dissociation of the Se-H bond. The Se-H bond is more easily dissociated than the S-H bond, leading to higher acidity of selenocysteine (Sec) compared to cysteine (Cys). This difference in acidity can be exploited to selectively modify the reactivity of peptides containing Sec. Furthermore, Se-H bonds in selenium-containing peptides are more susceptible to oxidation than their sulfur analogs. This property can be used to selectively modify the peptides by introducing new functional groups, such as disulfide bonds, which are important for protein folding and stability. These unique properties of selenium-containing peptides have found numerous applications in the field of chemical biology. For instance, selenium-containing peptides have been used in native chemical ligation (NCL). In addition, the reactivity of Sec can be harnessed to create cyclic and stapled peptides. Other chemical modifications, such as oxidation, reduction, and photochemical reactions, have also been applied to selenium-containing peptides to create novel molecules with unique biological properties.
Enhancement of Structural, Optical and Photoelectrochemical Properties of n−Cu2O Thin Films with K Ions Doping toward Biosensor and Solar Cell Applications
Authors: Abdelfatah Mahmoud , Darwesh Nourhan , Habib Mohamed A. , Alduaij Omar K. , El-Shaer Abdelhamid , Ismail Walid
Published: April 2023

n-type Cu2O thin films were grown on conductive FTO substrates using a low-cost electrodeposition method. The doping of the n−Cu2O thin films with K ions was well identified using XRD, Raman, SEM, EDX, UV-vis, PL, photocurrent, Mott–Schottky, and EIS measurements. The results of the XRD show the creation of cubic Cu2O polycrystalline and monoclinic CuO, with the crystallite sizes ranging from 55 to 25.2 nm. The Raman analysis confirmed the presence of functional groups corresponding to the Cu2O and CuO in the fabricated samples. Moreover, the samples’ crystallinity and morphology change with the doping concentrations which was confirmed by SEM. The PL results show two characteristic emission peaks at 520 and 690 nm which are due to the interband transitions in the Cu2O as well as the oxygen vacancies in the CuO, respectively. Moreover, the PL strength was quenched at higher doping concentrations which reveals that the dopant K limits e−/h+ pairs recombination by trapped electrons and holes. The optical results show that the absorption edge is positioned between 425 and 460 nm. The computed Eg for the undoped and K−doped n−Cu2O was observed to be between 2.39 and 2.21 eV. The photocurrent measurements displayed that the grown thin films have the characteristic behavior of n-type semiconductors. Furthermore, the photocurrent is enhanced by raising the doped concentration, where the maximum value was achieved with 0.1 M of K ions. The Mott–Schottky measurements revealed that the flat band potential and donor density vary with a doping concentration from −0.87 to −0.71 V and 1.3 × 1017 to 3.2 × 1017 cm−3, respectively. EIS shows that the lowest resistivity to charge transfer (Rct) was attained at a 0.1 M concentration of K ions. The outcomes indicate that doping n−Cu2O thin films are an excellent candidate for biosensor and photovoltaic applications.
The Association between Caffeine Intake and the Colonic Mucosa-Associated Gut Microbiota in Humans—A Preliminary Investigation
Authors: Dai Annie , Hoffman Kristi , Xu Anthony A. , Gurwara Shawn , White Donna L. , Kanwal Fasiha , Jang Albert , El-Serag Hashem B. , Petrosino Joseph F. , Jiao Li
Published: April 2023

We examined the association between caffeine and coffee intake and the community composition and structure of colonic microbiota. A total of 34 polyp-free adults donated 97 colonic biopsies. Microbial DNA was sequenced for the 16S rRNA gene V4 region. The amplicon sequence variant was assigned using DADA2 and SILVA. Food consumption was ascertained using a food frequency questionnaire. We compared the relative abundance of taxonomies by low (<82.9 mg) vs. high (≥82.9 mg) caffeine intake and by never or <2 cups vs. 2 cups vs. ≥3 cups coffee intake. False discovery rate-adjusted p values (q values) <0.05 indicated statistical significance. Multivariable negative binomial regression models were used to estimate the incidence rate ratio and its 95% confidence interval of having a non-zero count of certain bacteria by intake level. Higher caffeine and coffee intake was related to higher alpha diversity (Shannon index p < 0.001), higher relative abundance of Faecalibacterium and Alistipes, and lower relative abundance of Erysipelatoclostridium (q values < 0.05). After adjustment of vitamin B2 in multivariate analysis, the significant inverse association between Erysipelatoclostridium count and caffeine intake remained statistically significant. Our preliminary study could not evaluate other prebiotics in coffee.