bluuebunny/crossref_metadata_2025 huggingface dataset:
IbM BK teacher for SMA and its equivalent in Sidoarjo District
Authors: Hardiansyah Eko , Mariyati Lely Ika
Published: January 2022

The role of guidance and counseling teachers is very strategic in school programs. Guidance and counseling activities have many important functions for the development of students in schools which ultimately lead to an increase in academic achievement and a decrease in student behavior problems. The goal of IbM BK teachers for high school and equivalent in Sidoarjo District is to improve the quality of BK teacher services. The first specific target is to increase the capacity of BK teachers in conducting individual group counseling using choice theory. The second is the provision of student development books so that BK teachers have a source of information to communicate student problems with parents, principals, subject teachers, and other parties such as psychologists or psychiatrists for sustainable treatment. The third is an increase in the network of cooperation between schools and the Sidoarjo Health Office regarding referral services in severe cases that are difficult for BK teachers to handle alone. The results obtained are 1) BK teachers have started using individual and group counseling in handling students on the basis of choice theory; 2) They have also begun to be able to use the principle of choice theory in interacting with other students and teachers so that students seem less tense when dealing with BK teachers; 3) By applying the principles of choice theory, BK teachers feel that the counseling carried out can be more directed, structured, and the impact of change is more visible on the students they treat; 4) BK teachers can conduct classroom action research (CAR) using the instruments provided in the student development book; 5) With the SOP for Transfer of Hands, teachers can have an idea of ​​the flow and parties who need to be invited to work together if they get students with severe psychological problems that are difficult to handle on their own, although currently they have not yet received such cases.
Exploratory Study on the Importance of Counseling Guidance Services for Students at UMSIDA
Authors: Nastiti Dwi , Habibah Nur
Published: January 2022

This research conducted because of many college student have problems associated with their learning process, examples : learning achievement down, relation problems with their parent or friends, adaptation with new environment or rules, etc., but theres no right place, like guidance and counseling, that can help have the college student that have difficulty solving their problems at the college. There 4 (four) aims of this research are : (1) If guidance and counseling services needed, (2)  Whether college student will take advantage of the guidance and counseling services, (3)  What kind of guidance and counseling services they need, (4) What factors that make student college need guidance and counseling services.The variable of this research is the need of guidance and counseling services at the college. This variable means the need of psychological assistance from  professionals who have the expertise and authority to give guidance and counseling servives to individu who are in college which aims to help solving problems so that the student develop optimally in accordance with their potential.The population of this research are 6.163 college student of Univesitas Muhammadiyah Sidoarjo 2014-2015. The sample taken with the technique non probability sampling taken using incidental sampling method. Determination of the number of samples based on the Issac Michael Table (with standart error of 1%) and representative samples obtained as 598 student, but samples collected 638 student.The analysis that used in this research is descriptive analysis with analysis of the percentage. The result show that 77,4% students  think that the guidance and college services required at college, and  78,06% students will utilize the available guidance and counseling services.  The form of services that most needed is Guidance and counseling services (19,2%), dan  18,37% student will utilize the services when they have a difficult problem solved.
The Relationship between Self Control and Job Stress with Police Aggressiveness
Authors: Putra Andra Purnama , Rini Rr. Amanda Pasca , Saragih Sahat
Published: January 2022

Aggression is a behavior that is intentionally to hurt,threaten, or endanger others or another object physically or verbal and direct or indirect. Aggression is a common characteristic that can’t be erase from human life, especially police. All police has it own aggression, but the capacity is different with others police depend on self control and work stress they have. The research purpose’s to know correlation of self control (X1) and work stress (X2) with police aggression (Y). It subject is sergeant policeman in Sidoarjo. Subject amount 120 Police. The sampel taken by purposive sampling method. Statistical analysis method that be used is regression and using SPSS 22.00 for windows. the first result is there is correlation between self control, work stress and Police aggression.the second result is there is correlation negatively between self control and aggression. The third result is there is correlation positively between work stress and aggression.
Husband's Social Support for Mothers With Postpartum Depression After Giving Birth to Their First Child
Authors: Sari Dian Mustika , Ansyah Eko Hardi
Published: January 2022

This study aims to provide an overview of social support her husband with postpartum depression in mothers postpartum first child and the impact of social support provided to the mother's husband. Social support is in the form of emotional support, the support award, instrumental support and informative support. The method used in this research is using qualitative exploratory study the two subjects who had a husband and wife with postpartum depression after giving birth first child with wife aged 21-28 years. Determination of the subjects in this study using purposive sampling technique and is located in several places according to the agreement of the subject and significant others. Data collection methods used were observation method using anecdotel record with the observation that social support indicators husband and interview methods are equipped with general guidance interview. The results showed that each subject provide social support her husband with different forms of support for marriage influenced by the subject. The effect of the second wife of the study subjects also differ on the subject of the first wife can get through 25 days postpartum depression after giving birth, while the second subject can get through 40 days postpartum depression after giving birth. Factors support from significant others and coping also affect maternal postpartum depression can get through the second wife of the research subjects.
Anxiety and Self-Reinforcement in Advanced Semester Students
Authors: Anwar Zainul , Fajri Silviana Nur
Published: January 2022

Students who have not completed their study within the specified time limit indicated that they have psychological problems during doing the final task that trigger their anxiety. While the anxiety experienced by Students correlated with the frequency of self-reinforcement against them. This study aimed to determine the differences in the level of anxiety in terms of the frequency of self-reinforcement to Students who exceed the time limit. This study used quantitative methods to collect comparative data through the Taylor's Manifest Anxiety Scale instrument and Frequency of Self Reinforcement Questionnaire. The number of subjects were 123 students. The results showed that there were differences in anxiety levels significantly between students who have a high frequency of self-reinforcement tostudents with low frequency of self-reinforcement.
Psychological Contract and Employee Work Motivation of PT. Ark of the Krian Brothers
Authors: Cahyo Agus Dwi
Published: January 2022

Work motivation can affects employee productivities that can be supported by the expectations that are desired the employee through the psychological contract. This study aims to investigate the relationship between psychological contract with employee motivation. This study used a quantitative correlational approach using the psychological contract as an independent variable (X) and work motivation as the dependent variable (Y). Sampling techniques used proportionate stratified random sampling of 80 employees of PT. Primary Wisesa Mandiri. The data analysis used a correlational technique of product moment pearson which is calculated with SPSS 16.0 for Windows, obtained the correlation coefficient (r) = 0.446; ρ = 0.001 <ρ = 0.01. Correlation value is positive, it means there was a significant relationship, ie, the higher the psychological contract owned by the higher employee motivation.
Constructive Communication Patterns of Students in Facing Psychological Pressure in Completing Final Projects
Authors: Muzni Achmad Irfan , Wicaksono Awang Setiawan
Published: January 2022

Generally most students are feeling tense and depressed in finishing the final project. This condition if it doesn’t proportionately respond it will cause severe reactions such as depression feeling that will make them can’t complete their studies. This research is aiming to obtain a precise pattern of constructive communication among students in order to maintain the spirit when they faced psychological pressure while completing the final project. This study was matching methods approach which conducted at higher education institutions in Gresik. The subjects were the students who are taking the final project. Data collection did by Report Cards Card System, depth interviews and Focus Group Discussion. The data was analyzed by triangulation method. Research found in facing of psychological distress, students develop social communication patterns by opening discussion forum, telling the problem directly, asking for technical assistance, keeping and grumbling the problems and isolate their self. The constructive communication patterns that can be developed by students is providing mutual support group, sharing experiences, maintaining communication with the supervisor, discussing the issues, asking each other’s progress, and pulling away to reduce the exchange of information in order to maintain concentration in finishing the final project.
Work Spirit and Implementation of Nursing Care at the Kolaka General Hospital, Southeast Sulawesi
Authors: Esita Zulaeni
Published: January 2022

The implementation of nursing care that has not been done optimally, it has became a common problem in the most of government hospitals in Indonesia. Many customers feel not dissatisfied for health care services received from government hospitals. Work motivation is considered as one of the factors that influence the implementation of nursing care in hospital. It is can provide quality health services in hospital which expected by customers. The focus of this study was about how influence of Work motivation to the implementation of nursing care, especially in the Rumah Sakit Umum (RSU)Kolaka in Southeast Sulawesi. The study was designed as a quantitative. The population in this study were all nurses who worked at the RSU Kolaka. All the population in this study were the civil servants (PNS).The sampling technique used was purposive random sampling, so that the sample used in this research consisted of 53 nurses. Analysis of the data used was a simple linear regression analysis. The results of study showed thatthe work motivation contributed 15.6%to the implementation of nursing care (R2 = 0.156) in the RSU Kolaka Southeast Sulawesi.
Psychometric Analysis of Religiosity Scale in the Elderly
Authors: Amalia Sofa
Published: January 2022

Religiosity is deemed essential for humans, especially when humans entered the elderly. Increased relationship with their religion will improve the health and psychological well-being of the elderly. The purpose of this study is to conduct psychometric analysis of the religiosity scale based on the context of Islamic religion. The sample consisted of 205 elderly with aged criteria above 60 years. This scale was developed based on the multi-dimensional structure of religiosity theory, are: the ideological (acceptance of the belief system); the feeling (subjective and emotional religious experience as an expression of personal religiousness); the ritualistic (participation in religious activities and practices); the intellectual (knowledge of the belief system); and the consequential (ethical consequences of the belief system). The measurement method with reliability test uses Cronbach’s alpha and internal validity measurement using corrected item-total correlation and exploratory factor analysis (EFA). The results show that the scale has consistency and has items that are feasible to explain the variables of religiosity
Theory and Practice of a Flexible Fiber Optic Cable in a Horizontal Well Used for Cross-Well and Microseismic Hydraulic Fracture Monitoring
Authors: LeBlanc Michel , Suh Kwang , Machovoe Sean , Byrd Dane , Jaaskelainen Mikko , Bland Henry , Stokes Joshua , Henao Tito , Sahdev Neha
Published: January 2022

A flexible optical fiber cable, either as a wireline or a disposable fiber deployed using a pumped fiber payout shuttle, in a horizontal well, can be used be measure distributed near-static or dynamic strain. These measurements can be used to monitor the hydraulic fracturing treatment of nearby wells. It is the objective of this paper to present a theoretical framework for the understanding of the cable behavior and to compare it to field measurements.

The theory predicts the conditions under which slippage occurs between the optical fiber cable and the wellbore if coupling is provided by Coulomb friction. For near-static strain, as used in crosswell strain monitoring, the theory explains the broadening of the strain zone detected with a wireline cable. However, the theory underestimates the coupling provided by a grease-covered disposable fiber where a level of adhesion between the fiber and the wellbore provides better coupling than possible by Coulomb friction alone. Understanding the fundamental theory explains measured data and enables confident data interpretation regardless of sensing cable configuration.

For the dynamic strain, the theory confirms the generally good response observed using the flexible cables for microseismic monitoring due to the low amplitude of the dynamic strains involved. The low amplitude of the strains means that the strain gradients and inertial forces are also small, such that Coulomb friction is sufficient to provide the needed coupling.

An interesting result of the theory is the existence of a resonance condition allowing for large amplitudes to be detected faithfully even if only Coulomb friction is present. This resonance does not depend on signal frequency but on the match between the intrinsic travel speed of a disturbance on the cable and the apparent phase velocity of the seismic signal in the well direction.

Most importantly, the theory enables i) a comparison of different cable types for the near-static and dynamic strain applications, and ii) better data interpretation and associated decisions.

Field examples are provided to show both when the theory is applicable and where the obtained coupling exceeds what is predicted by the theory. The novel aspect of the paper is the first presentation of a theoretical background for the understanding of the performance of flexible cables inside horizontal wells used as static or dynamic strain sensors for the monitoring of hydraulic fracturing jobs.
Solar Farm Site Selection Using Spatial Multi Criteria Evaluation Method: Acase Study of Kewet Wereda, North-Center Ethiopia.
Authors: Goshem Gashaw Kibret , Hailu Binyam Tesfaw
Published: January 2022

Solar energy can provide a great opportunity for alternative energy source and development of the nation of the country. It can access freely throughout earth’s surface so, solar energy is the best alternative energy source for solving limitation of power especially when there is drought. Therefore, this thesis aims to select suitable solar farm sites by using spatial multi criteria evaluation method in Kewet Wereda. Different spatial and non-spatial data such as solar radiation, slope, aspect, LULC, proximity distance to road, proximity distance to sub-station site, proximity distance to river, proximity distance to railway and proximity distance to town have been used to identify and map area. Some parameter data are taken from 30 meter resolution of DEM such as solar radiation, slope, aspect and river streams. Analytical Hierarchy process (AHP) was used for Calculation of the criteria weighted overlay to produce a suitable solar farm site map. The result shows that solar radiation has the highest weight and distance to river has the lowest weight with 36.6% and 3.4%, respectively. In addition, optimal solar farm sites were identified and highly suitable area found in the south, southeast and northeast part of the study area and covers 35988.71 hectare area. Those potential zone contributes to fill the energy gap between the demand and supply of the country when there is a shortage of rainfall through the country and shortage of water at the dam.
Design of Noniterative Algorithms for Takagi Sugeno Kang Type General Type-2 Fuzzy Logic Systems
Authors: Chen Yang , Li Chenxi , Yang Jiaxiu
Published: January 2022

The paper performs the center-of-sets (COS) type-reduction (TR) and defuzzification for Takagi Sugeno Kang (TSK) type general type-2 fuzzy logic systems (GT2 FLSs) on the basis of the T-planes expression of general type-2 fuzzy sets. Actually, comparing the popular Karnik-Mendel (KM) algorithms with other noniterative algorithms is an important question in T2 society. Here the modules of fuzzy inference, COS TR, and defuzzification for TSK type GT2 FLSs are discussed by means of noniterative Nagar-Bardini (NB) algorithms, Nie-Tan (NT) algorithms, and Begian-Melek-Mendel (BMM) algorithms. Simulation instances are constructed to illustrate the performances of three types of noniterative algorithms compared with the KM algorithms. It is proved that, the proposed noniterative algorithms can enhance the computational efficiencies significantly, which afford the potential application value for designers of GT2 FLSs.
Transforming OpenAPI Specification 3.0 Documents into RDF-based Semantic Web Services
Authors: Muhamad Wardani , Suhardi Suhardi , Bandung Yoanes
Published: January 2022

Service-Oriented Architecture (SOA) designs are translated into software units and meet principles such as discoverable, reusable, and compostable. Web services are becoming a technology adopted by service providers so that services can be discovered and reused by clients. To complete more complex business processes, a set of existing services can be composed following a certain flow. To support client needs, these services should be equipped with comprehensive user interfaces in the form of web descriptions, which outlines the service capabilities regarding specific standard or language. This should be considered through an understandable format for humans and/or machines. In the Representational State Transfer (REST) architectural style, the OpenAPI Specification (OAS) is used as a reference to create web service descriptions. However, it only supports syntactic interoperability, leading to the incapability of supporting the automated selection process. The main challenges in this automation process should be overcome by providing web service semantics to create "understandable" services that automatically interact with one other. This indicates that the OAS document is improved by adding semantics to each resource. Therefore, this study aims to develop a system capable of transforming resources in OAS documents into RDF-based semantic web services. The OAS structure was the main basis for creating a relational database schema. Furthermore, a database normalization technique was implemented to design a schema, which stored all objects in an OAS document. To map resources on the RDF data model, an ontology was developed, explaining the concepts and the relationships between them. This adopted the published linked-open vocabulary and corresponded to the characteristics of each identified OAS resource. To build RDF-based semantic web services, R2RML was used to generate the relational database model into triple RDF. The proposed system was also tested through prototyping and using a dataset of 106 OAS documents, which were downloaded from APIs.guru between 5–10 May 2021. The number of triple RDFs generated from each document varied according to the rate of resources. This was observed when an OAS document produced 36 to 16,505 triple RDF in a specific dataset. The final result showed the formation of a knowledge base in triple RDF format, which was managed using a graph management database. This led to the utilization of the repository semantic web services as a basis for service selection (using SPARQL) so that service operations can be found as well as input and output parameters that correspond to the service composition requirements. The use of relational databases to store OAS resources also increased the efficiency of reuse by approximately 48%, due to service developer designing interoperability between uniform parameter services, which were subsequently used as input and output.
Effectiveness of Mindfulness-Based Happiness Pregnant Birth Parenting (HPBP) Programs on Mental Health During Pregnancy and Early Motherhood in China: A Multicenter Randomized Control Trial
Authors: Shulei Wang , Mengyun Sun , Xing Huang , Daming Zhang , Li Yang , Tao Xu , XiaoPing Pan , Ruimin Zheng
Published: January 2022

Background
This study aimed to investigate the efficacy of Happiness Pregnant Birth Parenting (HPBP) in preventing perinatal depression and reducing perceived anxiety and fear in Chinese pregnant women.
Methods
Women experiencing first-time pregnancy (n = 104) were randomly allocated to the HPBP group or a parallel control treatment group; We collected data at baseline (T0), post-intervention (T1), three days after delivery (T2), and 42 days after delivery (T3). Participants completed questionnaires regarding depressive symptoms, perceived anxiety, fear, satisfaction with life, and five facets of mindfulness. We analyzed differences between the two groups and changes within the same group at the four time points using repeated-measures ANOVA.
Results
Compared to the active control treatment group, the HPBP group reported lower levels of perceived depressive symptoms at T2 (p < .05, d = 1.83) and T3 (p = .01, d = 2.21); significantly reduced anxiety at T1 (p = .001, d = 5.31) and T2 (p = .003, d = 6.12); significantly reduced fear at T1 (p < .01, d = 14.95) and T2 (p = .04, d = 10.95); and significantly increased life satisfaction at T1 (p < .01, d = 3.86) and T3 (p = .015, d = 3.1) and self-reported mindfulness at T1 (p = .01, d = 6.81) and T2 (p < .01, d = 8.7).
Conclusions
The findings indicate that the HPBP intervention based on mindfulness effectively decreases the risk of perinatal depression, anxiety, and fear. Thus, this research enhances our knowledge of effective intervention strategies to promote mental well-being and prevent perinatal depression or other negative mental states among pregnant women.
Trial registration
:Chinese Clinical Trial Registry (ChiCTR):ChiCTR2000033149; The date of first registration is 24/ 05/ 2020.
Study on Urban Rainstorms Design Based on Multivariate Secondary Return Period
Authors: Zhang Jinping , Zhang Hang , Fang Hongyuan
Published: January 2022

In order to reveal the correlations between rainstorm characteristics variables, and make the return period of rainstorm more reasonable and objective, in this study, the joint distribution models of rainstorm elements are established by using copula theory based on the rainfall data in a Chinese megacity, Zhengzhou. Then their combined design values of the primary return period (PRP) and secondary return period (SRP) derived by the maximum probability method (MP) and same frequency method (SF) are obtained, finally using the Pilgrim & Cordery method (PC) the rainstorm pattern is designed. The results indicate that the calculation of rainstorm return period (RRP) with SRP is more reasonable compared with PRP. For the same RRP, the rainstorm volume (RV) of “Or” return period (ORP) type is largest, while the “And” return period (ARP) is smallest, and the RVs of Kendall return period (KRP) and survival Kendall return period (SKRP) are between them. For the KRP, the RVs calculated by MP and SF are pretty close, and their relative errors is between -5.84% and 4.69%. Compared to ORP, the rainstorm patterns of KRP can reduce the magnitude and investment of the stormwater infrastructure. Moreover, the rainfall of the designed rainstorm pattern of SKRP mainly concentrated before the rain peak in contrast with KRP.
Computational Methods for Predicting the Outcome of Thoracic Transplantation
Authors: C.G Raji , A.K Safna
Published: January 2022

Cardiac disease and the death rates due to coronary heart failure and cardiomyopathy are increasing. Thoracic transplantation is now a widely accepted therapeutic option for end-stage cardiac failure. The survival rate after the organ transplantation is crucial. Survival prediction after heart transplantation is a hot area of research. The use of conventional statistical techniques is computationally expensive and does not provide reliable solutions. Artificial Neural Networks based survival prediction helps surgeons make precise decisions and predict the best outcomes. The proposed system implements multi-layer perceptron algorithm, which shows good performance in survival prediction. We also implemented our work in the Radial Basis Function Network model to prove the accuracy of proposed model. For this research study, data were collected from United Network for Organ Sharing database and extracted the relevant thoracic transplantation survival prediction attributes with the help of suitable data mining techniques. We obtained an accuracy of 97.1% from the multi-layer perceptron model with the evaluation of various performance measures. In order to assure the validity of the proposed model we implemented the Radial Basis Function model and obtained an accuracy of 92.37%. We collated the accuracy of proposed survival prediction models with existing systems and proved that the proposed system appeared to be best for survival prediction with higher accuracy compared to 85.9% in the existing system. The outcome of the model will be an asset for the lifesaving procedures in the medical field.
Amplitude and Frequency of Wetting and Drying Cycles Drive N2 and N2O Emissions From a Subtropical Pasture
Authors: Friedl Johannes , Deltedesco Evi , Keiblinger Katharina , Gorfer Markus , De Rosa Daniele , Scheer Clemens , Grace Peter R , Rowlings David W
Published: January 2022

Changes in amplitude and frequency of wetting and drying cycles in pasture systems due to climatic variation and irrigation management are likely to affect magnitude and partitioning of N2 and N2O emissions. This study investigated the effects of irrigation frequency on N2 and N2O emissions from an intensively managed pasture in the subtropics. Irrigation volumes were estimated to replace evapotranspiration and were applied either once (Low-Frequency) or split into four applications (High-Frequency). To test for legacy effects, a large rainfall event was simulated at the end of the experiment. Over 15 days, 7.9 ± 2.7 kg N2+N2O-N ha−1 was emitted on average regardless of irrigation frequency, with N2O accounting for 25% of overall N2+N2O. Repeated, small amounts of irrigation produced an equal amount of N2+N2O losses as a single, large irrigation event. The increase of N2O emissions after the large rainfall event was smaller in the High-Frequency treatment, shifting the N2O/(N2O+N2) ratio towards N2. Our findings suggest that reduced soil-gas diffusivity predominantly drives N2O and N2 emissions following large wetting events, while microbial O2 consumption largely drives N2O and N2 emissions after small, repeated wetting events in high N turnover pasture soils. The abundance of marker genes for N cycling did not differ between treatments, indicating that N and C availability, not gene abundance determined magnitude and N2O:N2 partitioning after rainfall. The observed legacy effect suggests that increased irrigation frequency can reduce the environmental impact (N2O), but not overall magnitude of N2O and N2 emissions from intensively managed pastures.
CO2 Emissions, Legal Strength & Environmental Sustainability Policies; And FDI Nexus In Sub-Saharan Africa
Authors: Asiedu Michael , Aboagye Emmanuel Mensah , Arthur Benedict , Kyeremeh Gabriel
Published: January 2022

The study employed the two steps systems GMM technique to examine the effect of FDI inflow in 54 countries in Africa from 2004 -2020. The effects of FDI on the economy and growth are positive, provided that policymakers meet minimum conditions. The advantages of FDI are conditioned by the ability of the host country to assimilate technologies and by a local environment that is secure and transparent. The effects of FDI on the environment are inescapable. The results show that there is indeed a positive relationship between FDI inflows and CO2 emissions in Africa. The results also show that the intensity of CO2 emissions does depend on the increase in GDP growth (economic growth). However, with robust, sustainable environmental policies and legal quality, the effect of FDI inflow on CO2 emissions can be mitigated.
Identifying KRT20 as Key Gene in Lymphatic Metastasis of Head and Neck Squamous Cell Carcinoma
Authors: Zhang Yi-Fan , Huang Qiang , Huang Hui-Ying , Shen Yu-jie , Lau Hui-Ching , Guo Yang , Hsueh Chi-Yao , Ren Heng-Lei , Zhou Liang
Published: January 2022

Background
Head and neck squamous cell carcinoma (HNSCC) was the seventh-most-common cancer worldwide in 2018. Moreover, lymphatic metastasis (LM) closely relates to HNSCC’s prognosis and recurrence. However, mechanism driving LM remains unclear. Therefore, this study aimed to identify key genes in the LM of HNSCC.
Methods
We used The Cancer Genome Atlas (TCGA) to identify differentially expressed genes (DEGs) between LM and non-LM cases. Random Forest model, the Search Tool for the Retrieval of Interacting Genes (STRING), Cytoscape, and cytoHubba were used to identify hub genes in DEGs, including KRT20 (Cytokeratins 20). We analyzed survival and pan-cancer of KRT20 in the TCGA. After overexpressing KRT20 in HNSCC cell lines, migration and invasion were investigated. We analyzed KRT20’s expression in HNSCC tissue microarrays, as well as the survival and clinicopathological features.
Results
In total, we identified 243 DEGs—143 upregulated genes and 100 downregulated genes. Further analysis revealed KRT20 as a potential key gene associated with lymphatic metastasis and overall survival rates among patients with HNSCC. Overexpression of KRT20 increased migration and invasion ability of Tu686 and FD cells. Our analysis of tissue microarrays revealed an overexpression of KRT20 among N1+ patients. Survival analysis results and HNSCC tissue microarrays’ clinicopathological features were consistent with our analysis of the TCGA. Moreover, high level of KRT20 expression might suggest an adverse HNSCC prognosis. Additionally, our GSEA analysis revealed that KRT20 participates in many compounds’ metabolic pathways—including pathways related to tumorigenesis and development.
Conclusion
We proposed that KRT20 may be a key gene in HNSCC with LM.
Disinfection Strategies of Carbapenem-resistant Klebsiella Pneumoniae in a Healthcare Facility
Authors: Ni Lijia , Zhang Zhixian , Shen Rui , Liu Xiaoqiang , Li Xuexue , Chen Baiji , Wu Xiquan , Li Hongyu , Xie Xiaoying , Huang Songyin
Published: January 2022

Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections have become a major global public health challenge. Disinfectants play an important role in controlling horizontal transmission in healthcare facilities. However, the long and extensive routine use of disinfectants, and far less regulated, leading to concern about the development of disinfectant resistance. We detected the susceptibility of CRKP strains against seven kinds of disinfectants commonly used in clinic to guide and assist the infection control work in hospital. Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests of seven kinds of disinfectants (0.1% benzalkonium bromide, 4% aqueous chlorhexidine, 75% alcohol, entoiodine II, 2% glutaraldehyde, 2000mg/L chlorine-containing disinfectants and 3% hydrogen peroxide) were detected by broth dilution method. Three efflux pump genes (oqxA、oqxB、and qacE△1-sul1) were detected by PCR. Results: A total of 162 strains of non-repetitive CRKP strains were isolated in SYS Memorial Hospital from 2015 to 2019. The MBCs expressed by diluted multiples of 64 CRKP strains against the seven kinds of disinfectants were 1-128, 16-32768, 2-64, 16-4096, 4-64, 2-8 and 64-2048, respectively. Aqueous chlorhexidine should be ≥ 2.5 g/L when rinsing or gargling mucous membrane and wound, 0.1% benzalkonium bromide should avoided be diluted, and chlorine-containing disinfectants should be ≥ 1000 mg/L. The mean MIC value of aqueous chlorhexidine from intensive care unit (ICU) (0.0034%) was significantly higher than that from non-ICU (0.0019%) (p < 0.05). The positive rates of three efflux pump genes oqxA, oqxB and qacE△1-sul1 were 68.8% (44/64), 15.6% (10/64) and 89.1% (57/64), respectively.Conclusion: The CRKP strains isolated showed extensive resistance to clinically used disinfectants, and the concentration of aqueous chlorhexidine and chlorine-containing disinfectants needs to be increased compared with the current standards of the healthcare industry. It reminds us to strengthen the monitoring of disinfectant resistance to super drug-resistant bacteria.
Clinical Outcomes of Dual Trigger With Combination GnRH Agonist and hCG Versus Single Trigger With hCG Alone in Women Undergoing IVF/ICSI Cycles: a Retrospective Cohort Study With Propensity Score Matching
Authors: Dong Li , Lian Fang , Wu Haicui , Xiang Shan , Li Yuan , Wei Chaofeng , Yu Xiaona , Xin Xin
Published: January 2022

Background: Despite a large number of studies on the selection of trigger drugs, it remains unclear whether the dual trigger with human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH) agonist, compared to the single trigger with hCG alone, can improve the clinical outcome of patients undergoing assisted reproductive technology. Therefore, this study aimed to compare the laboratory and clinical outcomes of dual trigger versus single trigger.Methods: In this retrospective cohort study, we evaluated 520 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles between July 2014 and September 2020 at the Reproductive and Genetic Center of Integrative Medicine, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine. All patients underwent IVF/ICSI treatment with fresh embryo transfer using the GnRH antagonist protocol. We used propensity score matching to control for confounding variables and binary logistic regression analysis to determine the correlations between trigger methods and pregnancy outcomes. After propensity score matching, 57 cycles from each group were evaluated and compared for laboratory or clinical outcomes in this retrospective cohort study. Results: There was no significant difference in the number of oocytes retrieved, embryos available, top-quality embryos, or the rate of normal fertilization between the dual-trigger and single-trigger protocols, respectively. The implantation rate, clinical pregnancy rate, ectopic pregnancy rate, and live birth rate were also similar between the two groups, while the miscarriage rate (37.0% vs. 12.5%, p=0.045) was notably higher in the dual-trigger than the single-trigger group. Subsequent binary logistic regression analysis showed that age was a remarkably significant independent predictor of both clinical pregnancy rate (odds ratio=0.90, 95% confidence interval: 0.84–0.97, p=0.006) and live birth rate (odds ratio =0.89, 95% confidence interval: 0.82–0.97, p=0.005). Conclusions: Therefore, dual-trigger for final oocyte maturation might increase miscarriage rate, but in terms of the laboratory and other pregnancy outcomes such as clinical pregnancy rate or live birth rate, there was no evidence to show that dual trigger was superior to an hCG-trigger alone for patients undergoing GnRH-antagonist cycles with fresh embryo transfer. Trial registration: Retrospectively registered.
Magnetic phase diagram of the solid solution LaMn2(Ge1-xSix)2 (0 ≤ x ≤ 1) unraveled by powder neutron diffraction
Authors: Mudring Anja-Verena , Siebeneichler Stefanie , Ovchinnikov Alexander , Bosch-Santos Brianna , Cabrera-Pasca Gabriel A. , Flacau Roxana , Huang Qingzhen , Carbonari Artur W. , Ryan Dominic
Published: January 2022

The structural and magnetic properties of the ThCr2Si2-type solid solution LaMn2(Ge1−xSix)2 (x = 0.0 to 1.0) have been investigated employing a combination of X-ray diffraction, magnetization and neutron diffraction measurements, which allowed establishing a magnetic composition-temperature phase diagram. Substitution of Ge by Si leads to a compression of the unit cell, which affects the magnetic exchange interactions. In particular, the magnetic structure of LaMn2(Ge1−xSix)2 is strongly affected by the unit cell parameter c, related to the separation of adjacent Mn layers. Commensurate antiferromagnetic layers and a canted ferromagnetic structure dominate the Si-rich part of the solid solution, whilst an incommensurate antiferromagnetic flat spiral and a conical magnetic structure are observed in the Si-poor part.
Relative Equilibrium and Stability of a Three-body Tethered Satellite in an Elliptical Orbit
Authors: Zhong Xue , Zhao Jie , Yu Kaiping , Xu Minqiang
Published: January 2022

This work focuses primarily on the relative equilibrium and stability of a three-body tethered satellite which center of mass moves along an elliptical orbit with arbitrary eccentricity in the central Newtonian field. We only consider the case with straight tethers, and the lengths of them are small compared to the size of the orbit. It means that the attitude motion of the satellite has no impact on its orbital motion. Additionally, it assumes that the lengths of the tethers are controllable. The law of the tether length control is determined on the basis of the dynamical model of the system so that there exists an Earth-pointing relative equilibrium of the tethered satellite. By analyzing the Floquet multipliers of the linearized system, Lyapunov instability regions and linear stability regions of the relative equilibrium are obtained in a plane of dimensionless parameters. Further nonlinear stability analysis is presented in the linear stability regions by means of a normal form technique and stability theorems on the Hamiltonian system. In particular, stability of nonresonant cases as well as fourth-order resonant cases are investigated in detail.
Caveolar Disruption with Methyl-β-Cyclodextrin Causes Endothelium-Dependent Contractions in Wistar Rat Carotid Arteries
Authors: Albrakati Ashraf
Published: January 2022

Caveolae are organizing centers for cellular signal transduction in endothelial cells (ED) and smooth muscle cells (SMCs) in the blood vessels. Methods: Myography was used to investigate effects of caveolar disruption using methyl-β-cyclodextrin (MBCD) on maxi-K channels in rat carotid arteries. Results: Incubation of carotid segments with MBCD augmented contractions in response to BaK (chemical channel agonist) but not those induced by depolarizing high potassium physiological saline (KPSS). In contrast, incubation with cholesterol-saturated MBCD (Ch-MBCD) abolished the effects of MBCD. Mechanical removal of endothelial cells by MBCD triggered a small contraction in response to BaK. Incubation with nitroarginine methyl ester (L-NAME) inhibited nitric oxide (NO) release, thereby causing increased contractions in response to BaK, and this effect was reversed by pretreatment with MBCD. These results suggest that MBCD inhibits endothelial NO release. Contrastingly, inhibition of maxi-K channels with iberiotoxin enhanced contractions in response to BaK. Likewise, L-NAME decreased the contractile effect of iberiotoxin, as in the ED-denuded arteries. Transmission electron microscopy (TEM) showed the presence and absence of caveolae in intact blood vessels before and after MBCD treatment, respectively, whereas histology confirmed ED removal after the treatment. Conclusions: Caveolar disrupted using MBCD impairs ED-dependent relaxation by inhibiting the release of NO from the ED and altered the contractility of SMCs independent of the ED due to reduced contribution of maxi-K channels to the SMC membrane potential, causing depolarization and increasing carotid artery contraction. These findings might help to understand physiological role of the maxi-K channels in rat carotid arteries.
Management and long-term outcomes of acute right colonic diverticulitis and risk factors of recurrence
Authors: Chen Ji , Ma Zhilong , Zhou Jia , Liu Weiwei , Yao Le , Xie Wangcheng , Su Mingqi , Yang Jin , Shao Jun
Published: January 2022

Background: Acute right-sided colonic diverticulitis (RCD) is a common disease in Asian populations for which the optimal treatment remains controversial. The aim of this study was to investigate management and evaluate long-term outcomes of treatment in patients with acute RCD. Methods: We retrospectively collected and analyzed clinical data for patients with acute RCD admitted to the Tongren Hospital, Shanghai Jiao Tong University School of Medicine from December 2015 to December 2020. The patients were divided into two groups, according to primary treatment strategy, which was either conservative treatment or surgical treatment. Results: A total of 162 consecutive patients with acute RCD were enrolled in the study. There was no significant difference in age, sex, history of abdominal surgery, medical co-morbidities, fever, previous history of RCD, treatment success rate and incidence of complications between the conservative and surgery groups. However, the recurrence rate in conservative groups was significantly higher than in surgery groups (16.53% vs 2.44%, P = 0.020). And more frequent bowel movements and previous history of RCD increased the risk of recurrence of acute RCD. Moreover, there was no significant difference in either treatment success rate or the overall recurrence rate between the patients with uncomplicated diverticulitis and patients with complicated diverticulitis. Conclusions: Surgical treatment is also safe and effective for acute RCD. Surgical treatment should mainly be considered for patients with acute RCD with recurrence risk factors (more frequent bowel movements and previous history of RCD) or with complicated acute RCD.
Drug Components-Disease Network Related to Acute Lung Injury Inference Based on Forest Graph-embedded Deep Feedforward Network
Authors: Yang Bin , Bao Wenzheng , Wang Jinglong , Chen Baitong
Published: January 2022

Background: Acute lung injury (ALI) is a serious respiratory disease, which can lead to acute respiratory failure or death. It is closely related to the pathogenesis of New Coronavirus pneumonia (COVID-19). Many researches showed that traditional Chinese medicine (TCM) had a good effect on its intervention, and network pharmacology could play a very important role. Results: In order to construct "disease-gene-target-drug" interaction network more accurately, deep learning algorithm is utilized in this paper. Two ALI-related target genes (REAL and SATA3) are considered, and the active and inactive compounds of the two corresponding target genes are collected as training data, respectively. Molecular descriptors and molecular fingerprints are utilized to characterize each compound. Forest graph embedded deep feed forward network (forgeNet) is proposed to train and identify 19 compounds in Erhuang decoction (EhD) and Dexamethasone (DXMS).Conclusions: The experiment results show that forgeNet performs better than support vector machines (SVM), random forest (RF) and gcForest.
Vascular and Neuronal Effects of General Anesthesia on Functional Magnetic Resonance Imaging
Authors: Vedaei Faezeh , Alizadeh Mahdi , Tantawi Mohamed , Romo Victor M , Mohamed Feroze B. , Wu Chengyuan
Published: January 2022

A number of functional magnetic resonance imaging (fMRI) studies rely on application of anesthetic agents that can modulate and complicate interpretation of the measured hemodynamic blood oxygenation level-dependent (BOLD) response. The purpose of this study was to investigate the effect of general anesthesia on two main components of BOLD signal including neuronal activity and vascular response. Breath-holding (BH) fMRI was conducted in wakefulness and under anesthesia states in patients with drug resistant epilepsy (DRE) who needed to get scanned under anesthesia during laser interstitial thermal therapy (LITT). BOLD and BOLD-cerebrovascular reactivity (BOLD-CVR) maps were compared between two states to assess the effect of anesthesia on neuronal activity and vascular factors. Overall, our findings revealed an increase in BOLD-CVR and decrease in BOLD response under anesthesia. The results proposed that the modulatory mechanism of anesthetics on neuronal and vascular components of BOLD signal may work in different ways. To our knowledge, this is the first human study to examine the effect of general anesthesia using BH fMRI imaging, enhancing our understanding of the effect of anesthesia on neuronal and vascular aspects of BOLD response and assisting the implication of general anesthesia and interpretation of outcomes in clinical setting.
Evaluation of fertility, hatchability and egg quality of indigenous chicken at different agro-ecologies of Sidama Region, Ethiopia
Authors: Tunsisa Legesse Yotona , Berihun Kefyalew
Published: January 2022

Poultry is one of the most important subsectors of livestock, supplying a cheap source of good quality animal protein in the form of meat and eggs. This study was conducted to assess egg fertility, hatchability and egg quality parameters of indigenous chickens in the Hulla, Aleta Wondo and Dale districts, representing highland, midland and lowland agroecologies, respectively. Six kebeles (two kebeles from each district) were purposively selected based on a lower distribution of exotic chickens. A total of 750 eggs of indigenous chicken were collected from three different agro-ecologies, 450 eggs were used for fertility and hatchability evaluation, and 300 eggs were used for internal and external quality analysis. The collected data were analyzed using SPSS version 20 and SAS version 9.0 packages. The results indicated that agroecology has no significant effect on the fertility and hatchability of indigenous chicken eggs. The values of egg weight, egg length, shell weight, yolk weight, and yolk diameter and albumen weight of eggs collected from midland were higher than those of eggs from highland and lowland areas. However, the highest values for albumen height, yolk height and Haugh unit were observed on eggs collected from highland agroecology. In conclusion, agroecology has no effect on the fertility and hatchability of indigenous chicken eggs. However, agroecology significantly affects the internal and external egg quality of indigenous chicken eggs. Therefore, the storage condition and duration of storage need consideration to maintain the external quality of eggs.