bluuebunny/crossref_metadata_2025 huggingface dataset:
DISCO-2 – an ambitious earth observing student CubeSat for arctic climate research
Authors: Dideriksen Andreas Kjær , Andersen Mads Fredslund , Priest Julian , Eriksen Nikolaj Forskov , Frandsen Mads Toudal , Melvad Claus , Rasmussen Tobias Frejo , Nielsen Noah Harry Kjeldgård , Strømsnes Cecillie Thorup , Ahlebæk Mads Juul , Samsing Sigrid , Larsen Thomas Buris , Don Jeppe , Pedersen Lasse Alexander Nissen , Jacobsen Rune Hylsberg , Rysgaard Søren , Kim Jung Min , Bayer Robert , Christensen Caroline , Christiansen Emil Fredsted , Gosvig-Leach Izabella Katharina , Gramstrup Rasmus , Hermansson Bóas , Hesselvig Jesper , Jakobsen Jonas Mariager , Kjellberg Daniel Gjesse , Lind Magnus Sejer , Lindhard Jeppe , Mikkelsen Mads , Millinge Oliver , Moltesen Tara Møller , Negendahl Sebastian Dahl , Nielsen Alexander Björn Kerff , Nielsen Gustav Balslev , Pedersen Maja Chieng Frisenberg , Platz Alexander Stegler Schøler , Rosero Paul , Savic Sofia , Sigurðsson Ívar Óli , Skjernov Gustav , Sørensen Nikolaj , Sørensen Samuel Grund , Theil Astrid Guldberg , Thomsen Jacob Bay , Valsted Nicolaj , Vestergaard Anna , Karoff Christoffer
Published: October 2024

The severe impact of global warming, especially in the arctic region, have a multitude of consequences spanning from sea-level rises and freshening of the ocean, to significant changes to the animal life, biodiversity and species distribution. As the arctic regions are inherently remote and can be both hazardous and difficult to reach, research to improve our understanding of the climate change impact is often limited to short term field-campaigns. Here we present the Danish DISCO-2 student CubeSat mission, designed to meet the growing need for an Earth-observing platform. This mission leverages the rapid advancements in CubeSat technology over the past decades to overcome the limitations of traditional fieldwork campaigns. DISCO-2 will assist on-going arctic climate research with a payload of optical and thermal cameras in combination with novel in-orbit data analysis capabilities. It will further be capable of performing photogrammetric observations to determine ice volumes from deteriorating glaciers and provide surface temperatures, enabling studies of heat transfer between glaciers and arctic fjords. As a student satellite, the payload capabilities will also be offered to novel student research ideas throughout the mission life time. The modularity and wide range of of-the-shelf-components for CubeSats has facilitated an immense opportunity to tailor this earth observing CubeSat to accommodate specific scientific goals and further provided students at the participating universities with an unparalleled possibility to go from an initial research idea to a running CubeSat mission.
Say No to Norovirus: What It Is and Why It Matters
Authors: Marino Andrew , Jekic Danica , Matharoo Jagtaj , Neville Siobhán
Published: October 2024

Norovirus is one of the most common causes of vomiting and diarrhea in the world and is often referred to as the “winter vomiting bug”. Scientists continue to study norovirus, as they aim to develop ways to treat and prevent it. In this article, we will tell you how norovirus infects people and how they feel when they are sick. When norovirus enters the human body, it takes over the cells and uses them to make more copies of itself. This mainly happens in the cells of the digestive system. Norovirus can cause people to have a fever, become tired, vomit, and have diarrhea. If the vomiting and diarrhea are persistent, then dehydration can result. This is often a greater risk in people who are older and living in nursing homes. Scientists have been working to develop new vaccines to protect people against norovirus by reducing the rate of infection.
An analytical framework to examine power in sustainable energy decision-making in cities
Authors: Basu Sumedha
Published: October 2024

Urban areas are emerging as “strategic sites” in the ongoing sustainable energy (SE) transitions. This has rekindled the importance of urban governments in initiating this transition urgently, a departure from the actors managing more mainstream centralized energy governance. However, while there is a growing international and academic interest in urban energy transitions, the political presence of cities in the global clean energy landscape remains largely underwhelming. Scholars studying urban energy transitions or governance have often pointed toward the lack of material and knowledge capacities of the urban governments as the key barrier for their muted actions. I argue that decision-making by urban governments with respect to clean energy adoption needs deeper inspection wherein aspects such as capacity, or the lack of it, are symptoms of underlying power contestations and conflicts that are negotiated in multi-level governance systems. The scholarship of power captures the ideas of contestation, control, and acquiescence, going beyond the ideas of cooperation prevalent in multilevel governance. In this article, I juxtapose these with concepts from multi-level governance and socio-technical studies to offer an analytical framework for understanding energy decision-making by city governments. The framework presented in this article attempts to capture both direct and indirect forms of power, their operationalization, and manifestation in constituting identities, actual decisions (and indecisions) as well as the conditions of decision-making. I also use the framework to understand the role of power in sustainable energy decision-making in three cities in India as an illustration of possible application of the framework.
Multidimensional strategies for sustainable management of cocoa by-products
Authors: Santos Lívia de Matos , Ferreira Ribeiro Camila Duarte , Alves Janaína de Carvalho , da Silva Isadora Santana Araújo , Silva Vanessa de Lima , Santos Isabelle Palma Patrício , Roselino Mariana Nougalli
Published: October 2024

Introduction

The limited understanding of the potential applications of cocoa by-products, due to the scarcity of studies that integrate scientific research and patent analysis, hinders the proper use of these by-products and compromises their role in promoting sustainability in the food industry.

Methodology

In this study, a multidimensional review encompassing scientific and patentometric analyses of cocoa by-products was conducted. Databases (Web of Science, Scopus, and Medline/PubMed) were utilized with search terms (Cocoa* OR Theobroma cacao AND by-product*) to identify scientific articles (144). Additionally, we explored earch databases [Espacenet and National Institute of Industrial Property (INPI)], employing a combination of earch terms and international classification codes (Cocoa* OR Theobroma cacao AND by-product* AND A23G), to identify the generated technologies (73).

Results and discussion

The results revealed a significant concentration of scientific research in the agricultural and biological sciences (68%), focusing on the bioactive and biofunctional composition of the cocoa by-products. Additionally, interest in technological innovation related to these by-products was identified, as evidenced by the increased number of patent registrations after 2020. These findings suggest a significant potential for growth in this sector and provide valuable insights for future research and product development within the food industry.
Effects of different hinge positions on tibial rotation in uniplanar medial opening wedge high tibial osteotomy with three-dimensional tibial models
Authors: Jing Lizhong , Ren Yulian , Wang Shaoshan , Yang Jiushan , Wang Jian
Published: October 2024

Background

To investigate the effects of different hinge positions in the sagittal and axial planes on distal tibial rotation (DTR) during medial opening wedge high tibial osteotomy (MOWHTO) with three-dimensional tibial models.

Methods

Preoperative CT data from 30 knee joints in 30 patients who underwent surgery for varus malalignment of knee were included. 1 standard hinge position (0°), 6 axial planes (±5°, ±10°, ±15°), and 6 sagittal planes (±5°, ±10°, ±15°) hinge positions were defined and virtual uniplanar osteotomy was performed. The correction angle of each model was generated using Fujisawa's point. Participants’ baseline characteristics, radiologic parameters and DTR were measured. One-Way Repeated Measures ANOVA and single factor linear regression analysis were used to analyze the association between tibial rotation and hinge position in the sagittal and axial planes.

Results

We found a clear linear correlation between changes in hinge position in the sagittal plane and DTR. The changes in DTR were the smallest when the hinge position was at 5°, where internal or external rotation of the DTR may occur. When the front aspect of hinge axis rotated distally, DTR tended towards internal. Meanwhile, when the front aspect of hinge axis rotated proximally, DTR tended towards external. There were no correlations with every hinge axis position in the axial plane.

Conclusions

It is sagittal but not axial hinge axis affects DTR in uniplanar MOWHTO with three-dimensional tibial models. In the sagittal plane, every change in hinge position was significantly linearly correlated with DTR. However, no linear correlations were observed between every hinge position change in the axial plane.
Donor-Specific Blood Transfusion in Lung Transplantation
Authors: Jin Xin , Pirenne Jacques , Vos Robin , Hooft Charlotte , Kaes Janne , Van Slambrouck Jan , Kortleven Phéline , Vandervelde Christelle , Beeckmans Hanne , Kerckhof Pieterjan , Carlon Marianne S. , Van Raemdonck Dirk , Looney Mark R. , Vanaudenaerde Bart M. , Ceulemans Laurens J.
Published: October 2024

Lung transplantation is still hindered by a high rate of chronic rejection necessitating profound immunosuppression with its associated complications. Donor-specific blood transfusion is a pre-transplant strategy aimed at improving graft acceptance. In contrast with standard stored blood or donor-specific regulatory T cells transfusions, this approach utilizes fresh whole blood from the donor prior to allograft transplantation, encompassing all cell types and plasma. The precise mechanisms underlying donor-specific blood transfusion-induced tolerance remain incompletely understood. Associations with regulatory/helper T cells, modulation of mononuclear phagocytic cells or microchimerism have been suggested. While numerous (pre-)clinical studies have explored its application in solid organ transplants like liver, kidney, and intestine, limited attention has been given to the setting of lung transplantation. This comprehensive review summarizes existing knowledge on the mechanisms and outcomes of donor-specific blood transfusion in solid organ transplants both in preclinical and clinical settings. We also address the potential benefits and risks associated with donor-specific blood transfusion in the field of lung transplantation, offering insights into future research directions.
Real and Virtual Lecture Rooms: Validation of a Virtual Reality System for the Perceptual Assessment of Room Acoustical Quality
Authors: Guastamacchia Angela , Rosso Riccardo Giovanni , Puglisi Giuseppina Emma , Riente Fabrizio , Shtrepi Louena , Astolfi Arianna
Published: October 2024

Enhancing the acoustical quality in learning environments is necessary, especially for hearing aid (HA) users. When in-field evaluations cannot be performed, virtual reality (VR) can be adopted for acoustical quality assessments of existing and new buildings, contributing to the acquisition of subjective impressions in lab settings. To ensure an accurate spatial reproduction of the sound field in VR for HA users, multi-speaker-based systems can be employed to auralize a given environment. However, most systems require a lot of effort due to cost, size, and construction. This work deals with the validation of a VR-system based on a 16-speaker-array synced with a VR headset, arranged to be easily replicated in small non-anechoic spaces and suitable for HA users. Both objective and subjective validations are performed against a real university lecture room of 800 m3 and with 2.3 s of reverberation time at mid-frequencies. Comparisons of binaural and monoaural room acoustic parameters are performed between measurements in the real lecture room and its lab reproduction. To validate the audiovisual experience, 32 normal-hearing subjects were administered the Igroup Presence Questionnaire (IPQ) on the overall sense of perceived presence. The outcomes confirm that the system is a promising and feasible tool to predict the perceived acoustical quality of a room.
Current-Based Analysis and Validation of a Wheel–Soil Interaction Model for the Trafficability of a Planetary Rover
Authors: Shen Yan , Zou Meng , Cao Hongtao , Pan Dong , Yuan Baofeng , He Lianbin
Published: October 2024

The assessment of trafficability for planetary rovers in relation to non-geometric hazards is a crucial issue in deep space exploration. This study relies on terramechanics theory and incorporates actual data from Mars soil and rover parameters to develop a model that accurately represents the interaction between the rover’s wheels and Martian soil. Through numerical simulations, this model specifically investigates the relationship between the current of the rover’s wheel drive motor and factors such as slip ratio, soil pressure parameters, and soil shear parameters. Terrestrial experiments are also conducted to verify the precision of certain numerical calculations. The proposed wheel–soil interaction model, based on wheel motor current, provides a foundation for assessing non-geometric trafficability and the inversion of planetary soil parameters.
Design and Testing of an Offset Straw-Returning Machine for Green Manures in Orchards
Authors: Zhang Quanzhong , Zhao Jinfei , Yang Xiaowen , Wang Ling , Su Guangdong , Liu Xinying , Shan Chuang , Rahim Orkin , Yang Binghui , Liao Jiean
Published: October 2024

In order to solve the problems of traditional orchard-specific green manure crushing and returning machines, such as the single operation effect, root system damage, unsustainable green manure growth, and low utilization rate, an offset crushing–furrowing–burying–straw-returning machine was designed for green manures in orchards. Based on quadratic regression combination experiments, the Discrete Element Method (EDEM) was used to construct a discrete element model simulating the deep furrowing and burying processes of the furrowing and soil-covering device, where the advance speed, plow-shaped furrowing blade rotation speed, and furrowing depth were considered as experimental factors and the coverage rate was taken as an evaluation index, and then simulation analyses were carried out to obtain experimental data; Design-Expert was used to perform ANOVA and RSM analyses, thus finding that its optimal working parameter portfolio consists of the advance speed of 42 m/min, the furrowing blade rotation speed of 300 r/min, and the furrowing depth of 190 mm, and that the coverage rate is 95.82% when this parameter portfolio is applied. Field experiments were conducted to validate the optimal parameter portfolio. The experimental results show that with an average coverage rate of 90.87% (4.95% away from the optimal value based on the simulation experiments on average), an average crushing length qualification rate of 91.24%, and an average root system damage rate of 5.6%, this device is applicable for its operation conditions. The development of this machine and the construction of its parameter model can provide a certain reference value for developing and optimizing related machines including green manure-returning machines.
Enhancing Enzymatic Hydrolysis of Rice Straw by Acid-Assisted Mechanocatalytic Depolymerization Pretreatment
Authors: Yu Pengpeng , Yu Haizhang , Cheng Junhui , Nie Jingru , Liu Yuxin , Niu Qijian , Yang Qizhi , Liu Yucheng , Ji Guanya
Published: October 2024

The inherent complexity of cellulose, hemicellulose, and lignin contributes to the recalcitrance of lignocellulosic biomass, resulting in a low conversion efficiency and high cost of bioethanol conversion. Pretreatment methods that disrupt the plant cell structure of lignocellulose, such as straw, can significantly enhance the conversion efficiency. In this study, we utilized an acid-assisted mechanocatalytic depolymerization technique to pretreat rice straw, and the results demonstrated a significant disruption of the cellulose structure of the straw. Compared to the untreated straw, the particle size of pretreated straw reduced from 279 μm to 11.8 μm, the crystallinity of cellulose decreased from 43.05% to 22.71%, the specific surface area increased by 177%, and the surface oxygen-to-carbon ratio (O/C) ratio was enhanced by 75%. The changes in microstructure enabled the pretreated straw to achieve a total sugar yield of over 95% within 12 h of enzymatic hydrolysis, significantly superior to the 36.24% yield from untreated straw, the 45.20% yield from acid impregnated straw, and the 73.25% yield from ball milled straw. Consequently, acid-assisted mechanocatalytic depolymerization emerges as a highly effective pretreatment strategy to enhance both the enzymatic hydrolysis and the overall conversion efficiency of rice straw.
Enhancing Antioxidant Benefits of Kombucha Through Optimized Glucuronic Acid by Selected Symbiotic Fermentation Culture
Authors: Chou Yu-Chieh , Lin Hui-Wen , Wang Chung-Yi , Hsieh Chen-Che , Santoso Shella Permatasari , Lin Shin-Ping , Cheng Kuan-Chen
Published: October 2024

Kombucha, a functional beverage rich in glucuronic acid, is fermented in the presence of acetic acid bacteria and yeast as the primary microorganisms. Glucuronic acid is recognized for its various physiological benefits, such as detoxification, antioxidation, and anti-inflammation. To optimize the glucuronic acid content in kombucha, various strain combinations by selecting fermented sources were accomplished. According to the experimental results, kombucha produced through co-fermentation with Pichia anomala and Komagataeibacter hansenii, with glucose-added black tea as the carbon source, exhibited the highest glucuronic acid production. A response surface methodology found that under optimized conditions of a 12.27% (w/v) carbon source concentration, a 10.07% (w/v) substrate concentration, and a 28.4 °C temperature, the highest glucuronic acid production reached 80.16 g/L, which represented a 2.39-fold increase compared to the original kombucha. Furthermore, the total polyphenol content increased by 3.87-fold, while DPPH and ABTS free radical–scavenging capacities increased by 1.86- and 2.22-fold, respectively. To sum up, these observations reveal the potential for commercial production of glucuronic acid–enriched kombucha and contribute to the development of functional food products related to kombucha in the future.
NFC Tag Awareness and Its Visual Implementation on Food Packaging: Case Study in Slovenia
Authors: Žurbi Tina , Gregor-Svetec Diana
Published: October 2024

Many companies are using smart packaging to provide consumers with more information about their products. The goal of our case study is to access the Near Field Communication (NFC) tag knowledge, as well as getting insights into tag positioning in food package design for better visibility in order to develop some guidelines for future tag implementations. A preliminary survey of professionals provided an overview of NFC tag usage, followed by an online survey that assessed knowledge and visibility of tag placements. These findings were further discussed in focus groups and measured using eye-tracking technology. For placement visibility assessments, well-known and fictitious packaging designs of milk were used. The results show that, due to the NFC tag’s low market penetration, consumer recognition is low, with only generations Y and Z being familiar with the NFC tag. Knowledge of the NFC tag does not significantly vary based on education level. When considering the use of NFC tags, it is crucial to define and understand the target market. If the target is younger generations, the potential to increase engagement with the product can be achieved. Additionally, to boost consumer interaction, NFC tags or other smart elements should include an activation prompt, positioned on the central right section of the packaging and distinguished by a distinct color. The influence of the design on tag visibility is essential to ensure its effectiveness.
Multi-Dimensional Fuzzy Clustering-Based Trajectory Initialization Algorithm for Infrared Weak Target Trajectories in Robust Clutter Environments
Authors: Yang Ziqian , Nie Hongbin , Li Yiran , Bian Chunjiang
Published: October 2024

When conducting maneuver target tracking, trajectory initialization plays a crucial role in enhancing the accuracy of tracking algorithms. During maneuver target tracking, the accuracy of the tracking algorithm can be significantly improved through trajectory initialization. However, the traditional trajectory initialization algorithms face issues such as susceptibility to noise interference, lack of universality, and poor robustness in environments with high clutter levels. To address these issues, this study proposes a trajectory initialization algorithm based on multidimensional fuzzy clustering (MDF-clustering). The algorithm utilizes multidimensional feature information of the target, such as speed and irradiance, to determine point trajectory affiliation by assigning weights based on the clustering center of each feature type. Subsequently, it updates the clustering center and weight assignment using the new target features, ultimately deriving the correct trajectory through iterative processes. Experimental results demonstrate that the proposed method achieves an average stable initialization frame number of 3.12 frames, an average correct trajectory initialization rate of 99.59%, an average false trajectory occupancy rate of 0.04%, and an average missed batch rate of 0.06%. These results indicate improvements of at least 0.87 frames, 27.11%, 60.28%, and 6.48%, respectively, in terms of initialization rate, false trajectory rate, and missed batch rate, when compared to traditional methods. The algorithm enhances the accuracy and robustness of trajectory initialization in challenging environments characterized by solid clutter and target maneuvers, offering significant practical value for target tracking in complex scenarios.
Lifecycle DoE—The Companion for a Holistic Development Process
Authors: Kunzelmann Marco , Wittmann Anja , Presser Beate , Brosig Philipp , Marhoffer Pia Kristin , Haider Marlene Antje , Martin Julia , Berger Martina , Wucherpfennig Thomas
Published: October 2024

Within process development, numerous experimental studies are undertaken to establish, optimize and characterize individual bioprocess unit operations. These studies pursue diverse objectives such as enhancing titer or minimizing impurities. Consequently, Design of Experiment (DoE) studies are planned and analyzed independently from each other, making it challenging to interlink individual data sets to form a comprehensive overview at the conclusion of the development process. This paper elucidates the methodology for constructing a Life-Cycle-DoE (LDoE), which integrates data-driven process knowledge through design augmentations. It delves into the strategy, highlights the challenges encountered and provides solutions for overcoming them. The LDoE approach facilitates the augmentation of an existing model with new experiments in a unified design. It allows for flexible design adaptations as per the requirements of subject matter experts (SME) during process development, concurrently enhancing model predictions by utilizing all available data. The LDoE boasts a broad application spectrum as it consolidates all data generated within bioprocess development into a single file and model. The study demonstrates that the LDoE approach enables a process characterization study (PCS) to be performed solely with development data. Furthermore, it identifies potentially critical process parameters (pCPPs) early, allowing for timely adaptations in process development to address these challenges.
A Co-Culture System for Studying Cellular Interactions in Vascular Disease
Authors: Padmanaban Abirami M. , Ganesan Kumar , Ramkumar Kunka Mohanram
Published: October 2024

Cardiovascular diseases (CVDs) are leading causes of morbidity and mortality globally, characterized by complications such as heart failure, atherosclerosis, and coronary artery disease. The vascular endothelium, forming the inner lining of blood vessels, plays a pivotal role in maintaining vascular homeostasis. The dysfunction of endothelial cells contributes significantly to the progression of CVDs, particularly through impaired cellular communication and paracrine signaling with other cell types, such as smooth muscle cells and macrophages. In recent years, co-culture systems have emerged as advanced in vitro models for investigating these interactions and mimicking the pathological environment of CVDs. This review provides an in-depth analysis of co-culture models that explore endothelial cell dysfunction and the role of cellular interactions in the development of vascular diseases. It summarizes recent advancements in multicellular co-culture models, their physiological and therapeutic relevance, and the insights they provide into the molecular mechanisms underlying CVDs. Additionally, we evaluate the advantages and limitations of these models, offering perspectives on how they can be utilized for the development of novel therapeutic strategies and drug testing in cardiovascular research.
Updated Review of Metal Nanoparticles Fabricated by Green Chemistry Using Natural Extracts: Biosynthesis, Mechanisms, and Applications
Authors: El-Seedi Hesham R. , Omara Mohamed S. , Omar Abdulrahman H. , Elakshar Mahmoud M. , Shoukhba Yousef M. , Duman Hatice , Karav Sercan , Rashwan Ahmed K. , El-Seedi Awg H. , Altaleb Hamud A. , Gao Haiyan , Saeed Aamer , Jefri Ohoud A. , Guo Zhiming , Khalifa Shaden A. M.
Published: October 2024

Metallic nanoparticles have found wide applications due to their unique physical and chemical properties. Green biosynthesis using plants, microbes, and plant/microbial extracts provides an environmentally friendly approach for nanoparticle synthesis. This review discusses the mechanisms and factors governing the biosynthesis of metallic nanoparticles such as silver, gold, and zinc using various plant extracts and microorganisms, including bacteria, fungi, and algae. The phytochemicals and biomolecules responsible for reducing metal ions and stabilizing nanoparticles are discussed. Key process parameters like pH, temperature, and precursor concentration affecting particle size are highlighted. Characterization techniques for confirming the formation and properties of nanoparticles are also mentioned. Applications of biosynthesized nanoparticles in areas such as antibacterial delivery, cancer therapy, biosensors, and environmental remediation are reviewed. Challenges in scaling up production and regulating nanoparticle properties are addressed. Power Point 365 was used for creating graphics. Overall, green biosynthesis is an emerging field with opportunities for developing eco-friendly nanomanufacturing platforms using abundant natural resources. Further work on optimizing conditions, standardizing protocols, and exploring new biosources is needed to realize the full potential of this approach.
Applications, Limitations, and Considerations of Clinical Trials in a Dish
Authors: Mir Amatullah , Zhu Angie , Lau Rico , Barr Nicolás , Sheikh Zyva , Acuna Diana , Dayal Anuhya , Hibino Narutoshi
Published: October 2024

Recent advancements in biotechnology forged the path for clinical trials in dish (CTiDs) to advance as a popular method of experimentation in biomedicine. CTiDs play a fundamental role in translational research through technologies such as induced pluripotent stem cells, whole genome sequencing, and organs-on-a-chip. In this review, we explore advancements that enable these CTiD biotechnologies and their applications in animal testing, disease modeling, and space radiation technologies. Furthermore, this review dissects the advantages and disadvantages of CTiDs, as well as their regulatory considerations. Lastly, we evaluate the challenges that CTiDs pose and the role of CTiDs in future experimentation.
Familial Pancreatic Cancer Research: Bridging Gaps in Basic Research and Clinical Application
Authors: Archasappawat Suyakarn , Al-Musawi Fatimah , Liu Peiyi , Lee EunJung , Hwang Chang-il
Published: October 2024

Familial pancreatic cancer (FPC) represents a significant yet underexplored area in pancreatic cancer research. Basic research efforts are notably limited, and when present, they are predominantly centered on the BRCA1 and BRCA2 mutations due to the scarcity of other genetic variants associated with FPC, leading to a limited understanding of the broader genetic landscape of FPC. This review examines the current state of FPC research, focusing on the molecular mechanisms driving pancreatic ductal adenocarcinoma (PDAC) progression. It highlights the role of homologous recombination (HR) and its therapeutic exploitation via synthetic lethality with PARP inhibitors in BRCA1/2-deficient tumors. The review discusses various pre-clinical models of FPC, including conventional two-dimensional (2D) cell lines, patient-derived organoids (PDOs), patient-derived xenografts (PDXs), and genetically engineered mouse models (GEMMs), as well as new advancements in FPC research.
Oncological Outcomes of Partial Gland Ablation Using High-Intensity Focused Ultrasound After Additional Confirmatory Transperineal Mapping Biopsy in Men with Prostate Cancer
Authors: Lee Jihwan , Song Wan
Published: October 2024

Background/Objectives: To evaluate whether additional confirmatory transperineal mapping biopsy (TPMB) in men with localized prostate cancer (PCa) alters the treatment plan and outcome of partial gland ablation (PGA) using high-intensity focused ultrasound (HIFU). Methods: We retrospectively reviewed data from 96 patients who underwent PGA using HIFU between January 2020 and June 2022. After multiparametric magnetic resonance imaging (mpMRI), all men underwent transrectal ultrasound (TRUS)-guided, cognitive-targeted biopsy and systematic biopsy. Men eligible for PGA using HIFU first underwent confirmatory TPMB. Any changes in the treatment plan after TPMB were analyzed. Follow-up TRUS-guided biopsy was performed 1 year post-operatively to evaluate oncological outcomes. Clinically significant PCa (csPCa) was defined as Gleason grade (GG) ≥ 2. Results: Among all subjects, the median age (IQR) was 65.0 (60.0–72.0) years and the prostate-specific antigen level was 5.20 (3.71–7.81) ng/mL. The results of both TRUS-guided biopsy and TPMB led to a change in the treatment plan (from unilateral to bilateral PGA) for 13 (13.5%) patients. The 1-year follow-up TRUS-guided biopsy identified PCa in 13 (13.5%) patients, and csPCa in 7 (7.3%) patients. The infield- and outfield-positive rates were 8.3% (8/96) and 3.1% (3/96), respectively, for any PCa, and 3.1% (3/96) and 2.1% (2/96), respectively, for csPCa. Conclusions: Confirmatory TPMB results in better disease identification and localization, thereby affecting the treatment plan and improving oncological outcomes. Therefore, confirmatory TPMB should be considered to establish an appropriate strategy for patients with localized PCa eligible for PGA using HIFU.
Impact of Formwork Materials on Concrete Surface Quality
Authors: Dapper Silvia Trein Heimfarth , Bersch Jéssica Deise , Masuero Angela Borges
Published: October 2024

Given the functional and aesthetic quality expected from concrete surfaces, this study investigated the influence of different formwork materials on their surface density, porosity, voids, and elementary chemical composition by relying on X-ray Microtomography (μCT), Scanning Electronic Microscopy (SEM), and Energy Dispersive X-ray Spectroscopy (EDS). The formwork materials assessed were galvanized steel, regular plywood (pink), marine plywood, polyvinyl chloride (PVC), and silicone. μCT showed that distinct formwork affected the surface density of the concrete. In this case, specimens cast within silicone and marine plywood had similar pore volumes although different pore sizes, whereas PVC led to the highest pore volume with small pore sizes. Galvanized steel and regular plywood resulted in similar porosity. SEM showed that the concrete surfaces produced with marine plywood formwork had the highest void content. EDS identified surface products resulting from the contact of concrete with the different formwork materials, suggesting the potential migration of chemical elements. This research significantly contributes to optimizing formwork material selection and enhancing concrete quality and durability. Moreover, it establishes a foundation for further investigations into how formwork materials affect concrete surfaces and the pathological manifestations potentially arising from the molding process.
Low-Temperature NH3-SCR Technology for Industrial Application of Waste Incineration: An Overview of Research Progress
Authors: Guo Qiannan , Zhao Minghu , Fan Hongzhao , Zhu Rongshu , Zhong Rigang , Bai Xianxiang
Published: October 2024

Selective catalytic reduction of nitrogen oxides with NH3 (NH3-SCR) was investigated deeper and deeper with poisoning factors such as H2O, SO2, heavy metals, etc. In order to remove the reheating process before the SCR reactor, the application trend of NH3-SCR technology in the non-power industry is concentrated on the condition of low temperature even ultra-low temperature. The present study summarizes the research process of SO2 and H2O resistance of NH3-SCR catalysts under low temperatures related to the working conditions of municipal solid waste incineration plants. In detail, the effects of a high content of H2O and low concentration of SO2 are reviewed. Other factors such as heavy metals, alkali, or alkaline earth metals in the reaction system, synergistic removal of NOx, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) are addressed. Finally, the catalytic performance of assembled monolithic catalysts and pilot-scale experiments are also analyzed for the possibility of industrial application. Hopefully, in view of the questions outlined in this study, valuable insights could be taken into consideration for the development of NH3-SCR in waste incineration.
Advancing CRISPR-Based Solutions for COVID-19 Diagnosis and Therapeutics
Authors: Hadi Roaa , Poddar Abhishek , Sonnaila Shivakumar , Bhavaraju Venkata Suryanarayana Murthy , Agrawal Shilpi
Published: October 2024

Since the onset of the COVID-19 pandemic, a variety of diagnostic approaches, including RT-qPCR, RAPID, and LFA, have been adopted, with RT-qPCR emerging as the gold standard. However, a significant challenge in COVID-19 diagnostics is the wide range of symptoms presented by patients, necessitating early and accurate diagnosis for effective management. Although RT-qPCR is a precise molecular technique, it is not immune to false-negative results. In contrast, CRISPR-based detection methods for SARS-CoV-2 offer several advantages: they are cost-effective, time-efficient, highly sensitive, and specific, and they do not require sophisticated instruments. These methods also show promise for scalability, enabling diagnostic tests. CRISPR technology can be customized to target any genomic region of interest, making it a versatile tool with applications beyond diagnostics, including therapeutic development. The CRISPR/Cas systems provide precise gene targeting with immense potential for creating next-generation diagnostics and therapeutics. One of the key advantages of CRISPR/Cas-based therapeutics is the ability to perform multiplexing, where different sgRNAs or crRNAs can target multiple sites within the same gene, reducing the likelihood of viral escape mutants. Among the various CRISPR systems, CRISPR/Cas13 and CARVER (Cas13-assisted restriction of viral expression and readout) are particularly promising. These systems can target a broad range of single-stranded RNA viruses, making them suitable for the diagnosis and treatment of various viral diseases, including SARS-CoV-2. However, the efficacy and safety of CRISPR-based therapeutics must be thoroughly evaluated in pre-clinical and clinical settings. While CRISPR biotechnologies have not yet been fully harnessed to control the current COVID-19 pandemic, there is an optimism that the limitations of the CRISPR/Cas system can be overcome soon. This review discusses how CRISPR-based strategies can revolutionize disease diagnosis and therapeutic development, better preparing us for future viral threats.
Fluorescent Carbon Dots with Red Emission: A Selective Sensor for Fe(III) Ion Detection
Authors: Fernández-Merino Ángela , Chávez Miriam , Sánchez-Obrero Guadalupe , Madueño Rafael , Blázquez Manuel , Del Caño Rafael , Pineda Teresa
Published: October 2024

We present a procedure for the synthesis and purification of p-phenylenediamine-based carbon dots that can be used for the recognition of Fe(III) ions. Carbon dots have an approximately spherical shape with an average size of 10 nm and are composed of a carbonaceous core surrounded by functional groups attached to it, both of which are responsible for their dual fluorescence properties. The emission bands have a different behavior, with a blue band dependent and a red emission independent of the excitation wavelength, respectively. Red emission is appropriate for the detection of ions and other molecules in biological environments because this high wavelength prevents the occurrence of processes such as resonance energy transfer and internal filter effects. In particular, the presence of Fe(III) ions produces an important quenching phenomenon that can be applied to the fabrication of a sensor. The platform is very sensitive, with a detection limit of 0.85 µM, which is within the lowest values reported for this ion, and a high selectivity that is believed to be due to the formation of a specific complex in the ground state through specific interactions of Fe (III) ions with pyridinic and amino groups on the surface of the nanomaterials.
Congenital Radioulnar Synostosis Review: Recommendations and Treatment Outcomes
Authors: Alabau-Rodriguez Sergi , Garrido Ferrer Jose Felix , Bulló Mir Xavier , Martín Dominguez Lidia Ana , Pardo Pol Albert , Soldado Carrera Francisco
Published: October 2024

Background/Objectives: Congenital radioulnar synostosis (CRS) is a rare congenital disorder of the elbow joint caused by the abnormal fusion of the radius and ulna during fetal development, leading to limited forearm rotation and functional impairment. This narrative review aims to summarize the key aspects of diagnostic suspicion, treatment options, and lifestyle management strategies for individuals affected by CRS. Relevant sections: While CRS often occurs sporadically, there are familial cases with an autosomal dominant inheritance pattern. The diagnosis is established through a combination of clinical evaluation and radiological imaging, which confirms the presence and extent of the synostosis. Identifying the specific type and severity of CRS is critical for management decisions. Surgical interventions are considered based on factors such as the patient’s age, level of functional limitation, and symptom severity, while conservative treatment may be appropriate for cases with mild impairment. Discussion: Various surgical techniques have been described, but derotation osteotomy has emerged as a preferred option due to its predictable improvement in forearm function. Nevertheless, surgical treatment poses challenges, including potential complications like nerve injury and recurrence of deformity. Cultural and individual considerations, such as the desired forearm position, must be addressed to achieve optimal outcomes aligned with the patient’s lifestyle and needs. Conclusions: Managing CRS requires a nuanced and individualized approach, recognizing the unique challenges each patient presents. This review highlights the importance of continuous research to refine diagnostic and therapeutic strategies, ultimately aiming to enhance functional outcomes and quality of life for CRS patients.
Evaluation of Physical and Mental Health in Adults Who Underwent Limb-Lengthening Procedures with Circular External Fixators During Childhood or Adolescence
Authors: Depaoli Alessandro , Magnani Marina , Casamenti Agnese , Ramella Marco , Menozzi Grazia Chiara , Gallone Giovanni , Viotto Marianna , Rocca Gino , Trisolino Giovanni
Published: October 2024

Background: Lower limb length discrepancy (LLD) in children and adolescents, often due to congenital or acquired conditions, is treated to achieve limb equality and alignment, optimizing function and minimizing cosmetic concerns for an active adulthood. This study evaluated the Health-Related Quality of Life (HRQoL) and physical functioning of adults who underwent unilateral limb lengthening with circular external fixators (EFs) in childhood. Methods: Fifty patients treated at a median age of 14.9 years completed the Short Form 36 (SF-36) and Stanmore Limb Reconstruction Score (SLRS) questionnaires in adulthood, with a median follow-up of 8.9 years. Results: Among the 50 patients, 38 underwent a single limb lengthening (21 tibia, 12 femur, 5 both), while 12 required multiple cycles. The median residual LLD was 0.4 cm, with 12 patients (24%) having over 2 cm. Complications occurred in 67% of procedures, mainly due to prolonged healing. Physical and mental health scores were significantly lower than normative data. The mean Physical Component Summary was 52.2 ± 7.2 (p = 0.20). The mean Mental Component Summary was 43.9 ± 8.6 (p = 0.001), notably lower in congenital LLD cases. Many SLRS items (Pain, Social, Physical Function, Work, and Emotions) strongly correlated with SF-36 items. Conclusions: Adults treated with distraction osteogenesis for congenital LLD show normal physical but lower mental health scores compared to peers. Lengthening procedure characteristics did not significantly impact mental health. Routine psychological and social assessments are recommended to prevent long-term distress by providing appropriate support.
Unveiling Tumorigenesis Mechanisms and Drug Therapy in Neuroblastoma by Mass Spectrometry Based Proteomics
Authors: Ren Keyi , Wang Yu , Zhang Minmin , Tao Ting , Sun Zeyu
Published: October 2024

Neuroblastoma (NB) is the most common type of extracranial solid tumors in children. Despite the advancements in treatment strategies over the past years, the overall survival rate in patients within the high-risk NB group remains less than 50%. Therefore, new treatment options are urgently needed for this group of patients. Compared with genomic aberrations, proteomic alterations are more dynamic and complex, as well as more directly related to pathological phenotypes and external perturbations such as environmental changes and drug treatments. This review focuses on specific examples of proteomics application in various fundamental aspects of NB research, including tumorigenesis, drug treatment, drug resistance, and highlights potential protein signatures and related signaling pathways with translational values for clinical practice. Moreover, emerging cutting-edge proteomic techniques, such as single cell and spatial proteomics, as well as mass spectrometry imaging, are discussed for their potentials to probe intratumor heterogeneity of NB.
Dental Caries in Children and Its Relationship with Parenting Styles: A Systematic Review
Authors: Moya-López María , Ruiz-Guillén Ana , Romero-Maroto Martin , Carrillo-Díaz María
Published: October 2024

Background: It can be affirmed that the parenting style of parents has an impact on the health-related behaviors of their children; the environment that parents create for their children can have an impact on both their habits and their oral health, and on the incidence of dental caries in children. The purpose of this study was to analyze the association between parenting style and childhood dental caries. Methods: Two researchers independently searched the English literature published up to May 2024 in four databases (PubMed, Web of Science, Scopus y Cochrane Library). The risk of bias was evaluated using the Modified Newcastle–Ottawa Quality Assessment Scale (NOS). This study is registered on PROSPERO (CRD42024573447). Results: Of the 130 studies identified, nine of them, with a total of 4250 participants, met our inclusion criteria. The evidence on the relationship between parenting styles and dental caries is varied. Of the studies reviewed, three showed a significant association between both factors, while four found no correlation, and two reported no significant differences in relation to parenting styles and the occurrence of dental caries. Conclusions: This discrepancy emphasizes the need for further research. Parenting styles impact child dental behavior on a global level, highlighting the relevance of recognizing these approaches in a dental context, given that parents have a fundamental role in guiding their children’s behaviors.
Microbial Dynamics in Periodontal Regeneration: Understanding Microbiome Shifts and the Role of Antifouling and Bactericidal Materials: A Narrative Review
Authors: Hashim Nada Tawfig , Babiker Rasha , Priya Sivan Padma , Mohammed Riham , Chaitanya Nallan CSK , Padmanabhan Vivek , El Bahra Shadi , Rahman Muhammed Mustahsen , Gismalla Bakri Gobara
Published: October 2024

Periodontal regeneration is a multifaceted therapeutic approach to restore the tooth-supporting structures lost due to periodontal diseases. This manuscript explores the intricate interactions between regenerative therapies and the oral microbiome, emphasizing the critical role of microbial balance in achieving long-term success. While guided tissue regeneration (GTR), bone grafting, and soft tissue grafting offer promising outcomes in terms of tissue regeneration, these procedures can inadvertently alter the oral microbial ecosystem, potentially leading to dysbiosis or pathogenic recolonization. Different grafting materials, including autografts, allografts, xenografts, and alloplasts, influence microbial shifts, with variations in the healing timeline and microbial stabilization. Biologics and antimicrobials, such as enamel matrix derivatives (EMD) and sub-antimicrobial dose doxycycline (SDD), play a key role in promoting microbial homeostasis by supporting tissue repair and reducing pathogenic bacteria. Emerging strategies, such as enzyme-based therapies and antifouling materials, aim to disrupt biofilm formation and enhance the effectiveness of periodontal treatments. Understanding these microbial dynamics is essential for optimizing regenerative therapies and improving patient outcomes. The future of periodontal therapy lies in the development of advanced materials and strategies that not only restore lost tissues but also stabilize the oral microbiome, ultimately leading to long-term periodontal health.
Performance of Drying Oil Modified Chinese Lacquer and Its Gilding Effect
Authors: Wang Qiuyi , Zhang Changjun , Wang Wei , Liu Xinyou
Published: October 2024

This study explores the modification of traditional Chinese lacquer by incorporating boiled tung oil (BTO), boiled linseed oil (BLO), and turpentine oil (TO) to enhance its properties for gold leafing applications. Current traditional lacquers are limited by slow drying times and inconsistent surface quality, making their performance suboptimal for decorative gilding. The research addresses these gaps by investigating how varying oil types and concentrations (10%, 30%, and 50%) affect the lacquer’s drying time, viscosity, leveling properties, and overall gilding performance. Results indicate that TO-modified lacquer exhibits the best overall performance, showing the fastest drying time, highest glossiness, and smallest color variation, while BTO provides the smoothest surface and BLO ensures the best adhesion. These results demonstrate that the careful selection of oil type and concentration significantly improves lacquer’s functionality for gold leafing, offering a more efficient and aesthetically superior alternative to unmodified lacquers. This study provides valuable insights for optimizing traditional lacquer formulations for modern applications in gilding and decorative finishes.