bluuebunny/crossref_metadata_2025 huggingface dataset:
Titanium alloys surface integrity of belt grinding considering different machining trajectory direction
Authors: Jiang Guiyun , Yang Hang , Xiao Guijian , Zhao Zeyong , Wu Yuan
Published: December 2022

The interplay of abrasive grains and materials complicates the grinding of titanium alloys by abrasive belts. Notably, the influence relationship of surface generation for complex curved workpieces such as hollow blades needs to be clarified, making precise control of the surface integrity of complex surfaces difficult in abrasive belt grinding applications. This paper thus proposes a trajectory planning method based on the direction of interaction between grinding grains and materials to reveal its influence law on the surface integrity of complex curved surfaces of titanium alloy with unevenly distributed machining allowances. First, a machining trajectory with different angles between the grinding direction and feed direction is proposed. In order to determine the corresponding experimental scheme for titanium alloy hollow blades. Experimental results are used to analyze the influence of different grinding trajectory directions on the surface roughness, residual stress, surface topography, and accuracy of the contours. The results show that different grinding trajectory directions significantly affect the workpiece’s surface integrity. By varying the grinding trajectory direction, it is possible to reduce the surface roughness of titanium alloy workpieces by approximately 40%, increase the surface residual compressive stress by approximately 50%, provide a finer workpiece surface and improve the consistency of the surface texture. This work is expected to guide the efficient and high-quality machining of complex curved parts such as titanium alloy hollow blades.
Untargeted metabolomics analysis reveals dynamic changes in co-fermentation with human milk-derived probiotics and Poria cocos
Authors: Wang Qishan , Yang Kai , Wei Xinyue , Qiao Weicang , Chen Lijun
Published: December 2022

Introduction

To develop functional foods with traditional medicines and homologous food ingredients as well as human milk-derived probiotics, the co-fermentation process of two probiotics, Lactobacillus plantarum R9 and Lactobacillus gasseri B1-27, isolated from the human milk of healthy parturients and the traditional medicine and food homologous ingredient Poria cocos, were separately investigated.

Results

The Poria cocos fermentation broth at 2.5% significantly enhanced the total number of L. plantarum R9 (p = 0.001) and L. gasseri B1-27 (p = 0.013) after 20 h of fermentation, and Non-targeted metabolomics assays conducted before and after fermentation of the human milk-derived L. plantarum R9 and L. gasseri B1-27 using the 2.5% Poria cocos fermentation broth revealed 35 and 45 differential metabolites, respectively. A variety of active substances with physiological functions, such as L-proline, L-serine, beta-alanine, taurine, retinol, luteolin, and serotonin, were found to be significantly increased. Mannitol, a natural sweetener with a low glycemic index, was also identified. The most significantly altered metabolic pathways were pyrimidine metabolism, pentose phosphate, yeast meiosis, ABC transporter, insulin signaling, and mineral absorption, suggesting that co-fermentation of human milk-derived probiotics and Poria cocos may affect the metabolism of trace minerals, sugars, organic acids, and amino acids.

Discussion

Overall, we determined that the optimal concentration of Poria cocos to be used in co-fermentation was 2.5% and identified more than 35 differentially expressed metabolites in each probiotic bacteria after co-fermentation. Moreover, several beneficial metabolites were significantly elevated as a result of the co-fermentation process indicating the valuable role of Poria cocos as a functional food.
Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China
Authors: Xu Xiandong , Fu Huiyun , Wan Guoyuan , Huang Jiangfeng , Zhou Zhiyong , Rao Yi , Liu Lihui , Wen Chungen
Published: December 2022

The area around Poyang Lake is the main aquaculture area in Jiangxi Province, China, and an important base for the supply of freshwater aquatic products. Aquaculture in the Poyang Lake area is severely threatened by diseases caused by bacterial pathogens, and Aeromonas veronii has been the main pathogen in recent years. In this paper, ERIC-PCR genotyping, virulence gene and antimicrobial resistance gene detection, and drug susceptibility tests were carried out on 46 A. veronii isolates obtained from aquaculture systems in the Poyang Lake area from 2016 to 2020. The results showed that the A. veronii strains in the Poyang Lake area had high genetic diversity, and 46 strains produced 36 ERIC genotypes. There were no geographical and temporal differences in the cluster analysis results and no dominant clones. All 13 virulence genes tested were detected, and all isolates had harbored 2 or more virulence genes, with a maximum of 12 virulence genes detected. Among the 22 antimicrobial resistance genes selected, 15 were detected; 97.8% of the isolates contained 2 or more antimicrobial resistance genes, with a maximum of 9 antimicrobial resistance genes. Drug susceptibility tests showed that some strains were resistant to a variety of traditionally effective drugs for Aeromomas, such as enrofloxacin and florfenicol. This study provides a reference for exploring the impact of aquaculture in the Poyang Lake area on public health.
FISHing for ciliates: Catalyzed reporter deposition fluorescence in situ hybridization for the detection of planktonic freshwater ciliates
Authors: Dirren-Pitsch Gianna , Bühler Dominique , Salcher Michaela M. , Bassin Barbara , Le Moigne Alizée , Schuler Martina , Pernthaler Jakob , Posch Thomas
Published: December 2022

Planktonic ciliate species form multiple trophic guilds and are central components of freshwater food webs. Progress in molecular analytical tools has opened new insight into ciliate assemblages. However, high and variable 18S rDNA copy numbers, typical for ciliates, make reliable quantification by amplicon sequencing extremely difficult. For an exact determination of abundances, the classical morphology-based quantitative protargol staining is still the method of choice. Morphotype analyses, however, are time consuming and need specific taxonomic expertise. Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) may represent a promising tool for the analysis of planktonic ciliates by combining molecular identification with microscopic quantification. We tested the applicability of CARD-FISH using nine cultured ciliate species. Eight species- and three genus-specific oligonucleotide probes were designed based on their 18S rRNA genes. The CARD-FISH protocol was adapted and the specificity of probes was established. We subsequently examined the precision of quantitation by CARD-FISH on single cultures and mock assemblages. Successful tests on lake water samples proved that planktonic ciliates could be identified and quantified in field samples by CARD-FISH. Double hybridizations allowed studying interspecific predator prey interactions between two ciliate species. In summary, we demonstrate that CARD-FISH with species-specific probes can facilitate studies on the population dynamics of closely related, small sized or cryptic species at high sampling frequencies.
Gut and lung microbiome profiles in pregnant mice
Authors: Wiscovitch-Russo Rosana , Taal Aji Mary , Kuelbs Claire , Oldfield Lauren M. , Ramar MohanKumar , Singh Harinder , Fedulov Alexey V. , Gonzalez-Juarbe Norberto
Published: December 2022

In recent years, microbiome research has expanded from the gastrointestinal tract to other host sites previously thought to be abacterial such as the lungs. Yet, the effects of pregnancy in the lung and gut microbiome remains unclear. Here we examined the changes in the gut and lung microbiome in mice at 14 days of gestation. Lung tissue and stool samples were collected from pregnant and non-pregnant female BALB/c mice, DNA was isolated, amplified, and bacterial specific V4 16S rRNA gene was sequenced. Using an in-house bioinformatic pipeline we assessed the microbial composition of each organ using stool and lung tissue samples. The stool data showed that Lachnospiraceae and Lactobacillaceae were more abundant in the pregnant mice. Likewise, Lactobacillaceae were dominant in the lungs of pregnant mice. However, Streptococcaceae were dominant in the lungs of non-pregnant mice with a low microbial abundance in the pregnant mice. A permutation test showed that pregnancy significantly contributes to the variance in both the lung and stool microbiome. At the same time, we estimate that 49% of the total detected operational taxonomic units were shared between the stool and lung data. After removing common stool-associated bacteria from the lung dataset, no microbial differential abundance was detected between the pregnant and non-pregnant lung microbial community. Thus, pregnancy contributes to variance to the lung and stool microbiome but not in the unique lung microbiota.
Utilizing apolipoprotein E genotypes and associated comorbidities for the assessment of the risk for dementia
Authors: Tung Hsin , Lin Ching-Heng , Chen Yi-Ming , Lee Wei-Ju , Chien Li-Sheng , Sun Ting-Hsuan , Liao Cai-Sian , Lin Yung-Yang , Hsiao Tzu-Hung
Published: December 2022

Introduction

Dementia is associated with many comorbidities while being related to Apolipoprotein E (ApoE) polymorphism. However, it is unclear how these clinical illnesses and genetic factors modify the dementia risk.

Methods

We enrolled 600 dementia cases and 6000 matched non-dementia controls, with identified ApoE genotype (ε4/ε4, ε4/ε3, and ε3/ε3). Eight comorbidities were selected by medical records, and counted if occurring within 3 years of enrollment.

Results

The dementia group had a higher ratio of carrying ε4 allele and prevalence of comorbidities than the non-dementia group. Homozygous ε4 carriers presented the broken line of dementia risk with the peak age at 65–75 years and odds ratio (OR) up to 6.6. The risk only emerged after 65 years of age in ε3/ε4 subjects with OR around 1.6–2.4 when aged > 75 years. Cerebrovascular accident (CVA) is the commonest comorbidity (14.6%). CVA, sleep disorder, and functional gastrointestinal disorders remained as significant risk comorbidities for dementia throughout all age groups (OR = 1.7–5.0). When functional gastrointestinal disorder and ε4 allele both occurred, the dementia risk exceeded the summation of individual risks (OR = 3.7 and 1.9 individually, OR = 6.0 for the combination). Comorbidities could also be predictors of dementia.

Conclusion

Combining the genetic and clinical information, we detected cognitive decline and optimize interventions early when the patients present a specific illness in a particular age and carry a specific ApoE allele. Of comorbidities, functional gastrointestinal disorder is the strongest predicting factor for dementia in ε4 allele carriers.
A pan-cancer analysis of the oncogenic role of zinc finger protein 419 in human cancer
Authors: Zhu Weizhen , Feng Dechao , Shi Xu , Li Dengxiong , Wei Qiang , Yang Lu
Published: December 2022

Background

As a ferroptosis-related gene, the polymorphism of zinc finger protein 419 (ZNF419) at the splice donor site may generate renal cell carcinoma-associated novel minor histocompatibility antigen ZAPHIR. However, the role of ZNF419 in prognosis and immunology in human tumors remains largely unknown. This study aimed to visualize the prognostic landscape of ZNF419 at pan-cancer level and explore the relationship between ZNF419 expression and the tumor immune microenvironment.

Method

Pan-cancer and mutation data were downloaded from TCGA databases and analyzed through R (version 3.6.4) and its suitable packages. Differential ZNF419 expression and prognosis were analyzed. Correlations with ferroptosis-related genes, pathway analysis, tumor stemness, heterogeneity, mutation landscape, and RNA modifications were also explored. The relationships between ZNF419 expression and tumor immunity were investigated through the TIMER and ESTIMATE methods.

Result

ZNF419 was differentially expressed between tumor and normal samples and was associated with overall survival, disease-specific survival and progression-free interval for STES, KIRC, LIHC, LUSC, PRAD, and BLCA. We found the interaction between ZNF419 and FANCD2 might involve in ferroptosis in pan-cancer level. In addition, the mutation frequencies of STES, KIRC, LIHC, LUSC, PRAD, and BLCA were 1.5%, 0.3%, 0.3%, 1.9%, 0.2%, and 0.7%, respectively. We detected that the expression of ZNF419 was closely correlated with most immune checkpoint genes and immune regulatory genes. Furthermore, we found that the ZNF419 expression level was negatively related to the immune score in the six cancers mentioned above. The expression of ZNF419 was significantly associated with various infiltrating immune cells, such as CD4+ T cells, CD8+ T cells, and macrophages in patients with KIRC, PRAD, and LUSC but was only significantly related to macrophages in BLCA patients.

Conclusion

ZNF419 might serve as a potential prognostic and immunological pan-cancer biomarker, especially for KIRC, LIHC, LUSC, PRAD, and BLCA.
Case report: Multicentric Castleman disease as a manifestation of immune reconstitution inflammatory syndrome in Malawi
Authors: Painschab Matthew S. , Mponda Marriam , Tomoka Tamiwe , Kampani Coxcilly , Chimzimu Fred , Fedoriw Yuri , Gopal Satish
Published: December 2022

Introduction

Multicentric Castleman disease (MCD) is a lymphoproliferative disorder characterized by systemic inflammation, lymphadenopathy, and cytopenias. MCD caused by Kaposi sarcoma herpesvirus (MCD-KSHV) frequently arises in the context of HIV. It can be associated with immune reconstitution inflammatory syndrome (IRIS), but MCD-IRIS is rarely reported in sub-Saharan Africa (SSA) where HIV and KSHV infection are common.

Case description

A 36-year-old woman in Malawi with HIV on antiretroviral therapy (ART) for nine years presented with fatigue, weight loss, and lymphadenopathy. Lymph node biopsy was consistent with HIV lymphadenitis without evident KSHV-MCD and HIV RNA was 4,244 copies/mL. She switched to second-line ART and returned four months later with worsening lymphadenopathy, fever, night sweats, weight loss, and anemia. A repeat lymph node biopsy demonstrated unequivocal KSHV-MCD features not present on the original biopsy. Her repeat HIV viral load was undetectable and she received chemotherapy with subsequent remission on continued ART for 24 months.

Discussion

This is among the first reported cases of MCD-IRIS from SSA, which has implications for a region where HIV and KSHV are highly prevalent. MCD-IRIS may contribute to early mortality after ART initiation in SSA, and increased awareness alongside improved diagnostic and treatment capacity are needed.
Effects of visual deprivation on the injury of lower extremities among functional ankle instability patients during drop landing: A kinetics perspective
Authors: Meng Lingyue , Kong Lintao , Kong Lingyu , Zhang Qiuxia , Shen Jianzhong , Hao Yuefeng
Published: December 2022

Background:The ankle is prone to injury during drop landing with usual residual symptoms, and functional ankle instability (FAI) is the most common. Vision guarantees the postural stability of patients with FAI, and visual deprivation (VD) increases their risk of injury when completing various movements. This study explored injury risk during drop landing in patients with FAI under VD through the kinetics of lower extremities.

Methods:A total of 12 males with FAI participated in the study (age, 23.0 ± 0.8 years; height, 1.68 ± 0.06 m; weight, and 62.2 ± 10.4 kg) completed single-leg drop landings under visual presence (VP) and VD conditions. Ground reaction force (GRF), time to peak GRF, joint torque, and vertical length variation (ΔL) were measured.

Results:Significant effects were detected in the group for time to peak lateral GRF (p= 0.004), hip extensor torque (p= 0.022), ankle plantarflexion torque (p< 0.001), ankle varus torque (p= 0.021), lower extremity stiffness (p= 0.035), and ankle stiffness (p< 0.001). Significant effects of conditions were detected for vertical GRF, time to peak vertical and lateral GRF, loading rate, hip extensor torque, knee extensor torque, hip varus torque, knee varus torque, lower extremity stiffness, and ankle stiffness (p< 0.05). ΔL was affected by VD with a significant difference (p< 0.001).

Conclusion:In patients with FAI, an unstable extremity has a higher injury risk than a stable extremity, and VD increases such risk. However, because the influence of the central nervous system on hip strategy is also affected, the effect on the unstable extremity is more significant and more likely to result in injury. Deepening the squat range may be an effective preventive measure for reducing injury risk of unstable extremities during drop landing.
Effects of leadership style on coach-athlete relationship, athletes’ motivations, and athlete satisfaction
Authors: Jin Hao , Kim Seungmo , Love Adam , Jin Yun , Zhao Jie
Published: December 2022

Introduction

The current study investigated the impacts of autocratic and democratic leadership styles on the coach-athlete relationship, athletes’ motivations, and athlete satisfaction.

Methods

Survey data were collected from 298 student-athletes (male = 157; 52.7%, female = 141; 47.3%) from 20 different Chinese collegiate sports. The Structural Equation Model was used to test the hypothesized model.

Results

The results indicated democratic leadership had a direct positive influence on the coach-athlete relationship, while autocratic leadership had no direct effect. Both leadership styles did not influence autonomous motivation. The coach-athlete relationship, meanwhile, had a full mediation effect between democratic leadership and athletes’ motivation and satisfaction. In addition, autonomous motivation had a partial mediation effect between the coach-athlete relationship and athlete satisfaction.

Discussion

Ultimately, the findings of the current study underscore the need for coaches and administrators to understand the impact of different leadership styles and highlight the importance of democratic leadership in improving athletes’ psychological outcomes.
Segmental and suprasegmental encoding of speaker confidence in Wuxi dialect vowels
Authors: Ji Yujie , Hu Yanbing , Jiang Xiaoming
Published: December 2022

Introduction

Wuxi dialect is a variation of Wu dialect spoken in eastern China and is characterized by a rich tonal system. Compared with standard Mandarin speakers, those of Wuxi dialect as their mother tongue can be more efficient in varying vocal cues to encode communicative meanings in speech communication. While literature has demonstrated that speakers encode high vs. low confidence in global prosodic cues at the sentence level, it is unknown how speakers’ intended confidence is encoded at a more local, phonetic level. This study aimed to explore the effects of speakers’ intended confidence on both prosodic and formant features of vowels in two lexical tones (the flat tone and the contour tone) of Wuxi dialect.

Methods

Words of a single vowel were spoken in confident, unconfident, or neutral tone of voice by native Wuxi dialect speakers using a standard elicitation procedure. Linear-mixed effects modeling and parametric bootstrapping testing were performed.

Results

The results showed that (1) the speakers raised both F1 and F2 in the confident level (compared with the neutral-intending expression). Additionally, F1 can distinguish between the confident and unconfident expressions; (2) Compared with the neutral-intending expression, the speakers raised mean f0, had a greater variation of f0 and prolonged pronunciation time in the unconfident level while they raised mean intensity, had a greater variation of intensity and prolonged pronunciation time in the confident level. (3) The speakers modulated mean f0 and mean intensity to a larger extent on the flat tone than the contour tone to differentiate between levels of confidence in the voice, while they modulated f0 and intensity range more only on the contour tone.

Discussion

These findings shed new light on the mechanisms of segmental and suprasegmental encoding of speaker confidence and lack of confidence at the vowel level, highlighting the interplay of lexical tone and vocal expression in speech communication.
Cardiovascular Disease Mortality and Potential Risk Factor in China: A Multi-Dimensional Assessment by a Grey Relational Approach
Authors: Rehman Shazia , Rehman Erum , Mumtaz Ayesha , Jianglin Zhang
Published: December 2022

Graphical Abstract
Influence of Aerodynamic Interaction on Performance of Contrarotating Propeller/Wing System
Authors: Zhang Zhitao , Xie Changchuan , Huang Kunhui , Yang Chao
Published: December 2022

This paper gives a quantitative account of the influence of slipstream on the aerodynamic performance of a contrarotating propeller (CRP)/wing system, and compares it with the CRP and clean wing. To accurately evaluate the complex aerodynamic interaction, the unsteady Reynolds-averaged Navier–Stokes approach using the sliding mesh method is performed at a typical freestream velocity of 30 m/s. Four different critical parameters, including the freestream angle of attack (AoA), axial spacing between the front propeller (FP) and rear propeller (RP), number of blades, and rotational speed, are considered in the present work. The results show that the thrust coefficient, power coefficient, and propulsion efficiency of the CRP/wing system change sharply and the difference in amplitude between adjacent waves is large. In particular, the propeller slipstream has a significant impact on the lift–drag performance of the wing in the case of a nonzero AoA. The presence of a wing also increases the efficiency of propulsion due to the recovery of vortices. In the case of a small axial spacing, the thrust coefficient value of the FP is significantly smaller than that of the RP. However, when the axial spacing exceeds a certain value, the opposite relationship is obtained. When the rotational speed increases from 3695 RPM to 8867 RPM, the lift coefficient and drag coefficient of the wing gradually increase.
Supplementing Diets with Agriophyllum squarrosum Reduced Blood Lipids, Enhanced Immunity and Anti-Inflammatory Capacities, and Mediated Lipid Metabolism in Tan Lambs
Authors: Jiao Dan , Liang Yanping , Zhou Shanshan , Wu Xiukun , Degen Abraham Allan , Hickford Jonathan , Zhou Huitong , Cong Haitao , Shi Xinxin , Ma Xiaofei , Yang Guo
Published: December 2022

Agriophyllum squarrosum (sand rice), a widespread desert plant, possesses anti-hyperglycemic and anti-inflammatory properties, and has been used in traditional Chinese medicine for many years. However, its effects on ruminants are unknown. To fill this gap, we examined the effects of A. squarrosum on the immune and anti-inflammatory responses of lambs. A total of 23, 6-month-old Tan ewe-lambs (27.6 ± 0.47 kg) were divided into four groups and offered a basic diet (C—control), or a diet that contained 10%, 20%, or 30% A. squarrosum, on a dry matter basis, for 128 days. Serum concentrations of total cholesterol were lower (p = 0.004) in the 30% supplemented lambs than controls, while concentrations of high-density lipoprotein cholesterol were lower (p = 0.006) in the 10% and 20%, but not in 30% supplemented lambs than controls. Serum-cortisol concentrations were lower (p = 0.012) in the 30% supplemented lambs and free fatty acid concentrations were higher in the 10% and 20% supplemented lambs than in control lambs (p < 0.001). Supplementation with A. squarrosum decreased (p < 0.05) the area of adipocytes in subcutaneous adipose tissue, but there was no difference between the 20% and 30% diets. Conversely, the area in visceral adipose tissue (VAT) increased (p < 0.05), especially for the 10% and 20% supplemented diets. Supplementation with A. squarrosum also enriched immune and anti-inflammatory related and lipid and glucose-metabolic pathways and associated differentially expressed gene expressions in adipose tissue. A total of 10 differential triacylglycerol, 34 differential phosphatidylcholines and seven differential phosphatidylethanolamines decreased in the diet with 30% supplementation, when compared to the other diets. Finally, adipocyte-differentiation genes, and immune and inflammatory response-related gene expression levels decreased in lamb adipocytes cultured with an aqueous A. squarrosum extract. In conclusion, supplementing lamb diets with A. squarrosum reduced blood lipids, enhanced immunity and anti-inflammatory capacities, and mediated lipid metabolism in adipose tissue and adipocytes of Tan lambs. A level of approximately 10% is recommended, but further research is required to determine the precise optimal level.
Assessment of Seasonal Variation in Methane Emissions of Mediterranean Buffaloes Using a Laser Methane Detector
Authors: Lanzoni Lydia , Chagunda Mizeck G. G. , Fusaro Isa , Chincarini Matteo , Giammarco Melania , Atzori Alberto Stanislao , Podaliri Michele , Vignola Giorgio
Published: December 2022

A direct assessment of the methane (CH4) emission level and its variability factors is needed in each animal species in order to target the best mitigation strategy for the livestock sector. Therefore, the present study aimed to (1) test a laser methane detector (LMD) for the first time in Italian Mediterranean buffaloes (IMB), a non-invasive tool to quantify CH4 emissions; (2) test the effect of season on the emissions; and (3) compare the results measured directly with the ones estimated with the existing equations. CH4 emissions of twenty non-productive IMB, under the same feeding regimen, were monitored for 12 days in summer and winter. Significantly higher THI (74.46 ± 1.88 vs. 49.62 ± 4.87; p < 0.001), lower DMI (2.24 ± 0.04 vs. 2.51 ± 0.03% DMI/kg live weight; p < 0.001) and lower emission intensities (0.61 ± 0.15 vs. 0.75 ± 0.13; p < 0.001) were found during the summer period when compared with winter. LMD was found to be a versatile tool to be used in buffaloes, and it was clear that a summer increase in THI could act as a stressor for the animals, influencing their emissions. In addition, measured emissions were significantly higher than when estimated with the existing equations (p < 0.001), suggesting the need for further research in this area.
Chicken LEAP2 Level Substantially Changes with Feed Intake and May Be Regulated by CDX4 in Small Intestine
Authors: Zheng Xiaotong , Chen Ziwei , Zhuang Wuchao , Zhang Jilong , He Jiaheng , Xie Yinku , Chen Jianfei
Published: December 2022

Ghrelin O-acyltransferase (GOAT), ghrelin, and GHSR have been reported to play important roles that influence feed intake in mammals. LEAP2, an endogenous antagonist of GHSR, plays an important role in the regulation of feed intake. However, chicken ghrelin has also been reported to have an inhibitory effect on feed intake. The role of the GOAT–Ghrelin–GHSR–LEAP2 axis in chicken-feed intake remains unclear. Therefore, it is necessary to systematically evaluate the changes in the tissue expression levels of these genes under different energy states. In this study, broiler chicks in different energy states were subjected to starvation and feeding, and relevant gene expression levels were measured using quantitative real-time PCR. Different energy states significantly modulated the expression levels of LEAP2 and GHSR but did not significantly affect the expression levels of GOAT and ghrelin. A high expression level of LEAP2 was detected in the liver and the whole small intestine. Compared to the fed group, the fasted chicks showed significantly reduced LEAP2 expression levels in the liver and the small intestine; 2 h after being refed, the LEAP2 expression of the fasted chicks returned to the level of the fed group. Transcription factor prediction and results of a dual luciferase assay indicated that the transcription factor CDX4 binds to the LEAP2 promoter region and positively regulates its expression. High expression levels of GHSR were detected in the hypothalamus and pituitary. Moreover, we detected GHSR highly expressed in the jejunum—this finding has not been previously reported. Thus, GHSR may regulate intestinal motility, and this aspect needs further investigation. In conclusion, this study revealed the function of chicken LEAP2 as a potential feed-intake regulator and identified the potential mechanism governing its intestine-specific expression. Our study lays the foundations for future studies on avian feed-intake regulation.
Identification of Copy Number Variations in Four Horse Breed Populations in South Korea
Authors: Kim Yong-Min , Ha Seok-Joo , Seong Ha-Seung , Choi Jae-Young , Baek Hee-Jung , Yang Byoung-Chul , Choi Jung-Woo , Kim Nam-Young
Published: December 2022

In this study, genome-wide CNVs were identified using a total of 469 horses from four horse populations (Jeju horses, Thoroughbreds, Jeju riding horses, and Hanla horses). We detected a total of 843 CNVRs throughout all autosomes: 281, 30, 301, and 310 CNVRs for Jeju horses, Thoroughbreds, Jeju riding horses, and Hanla horses, respectively. Of the total CNVRs, copy number losses were found to be the most abundant (48.99%), while gains and mixed CNVRs accounted for 41.04% and 9.96% of the total CNVRs, respectively. The length of the CNVRs ranged from 0.39 kb to 2.8 Mb, while approximately 7.2% of the reference horse genome assembly was covered by the total CNVRs. By comparing the CNVRs among the populations, we found a significant portion of the CNVRs (30.13%) overlapped; the highest number of shared CNVRs was between Hanla horses and Jeju riding horses. When compared with the horse CNVRs of previous studies, 26.8% of CNVRs were found to be uniquely detected in this study. The CNVRs were not randomly distributed throughout the genome; in particular, the Equus caballus autosome (ECA) 7 comprised the largest proportion of its genome (16.3%), while ECA 24 comprised the smallest (0.7%). Furthermore, functional analysis was applied to CNVRs that overlapped with genes (genic-CNVRs); these overlapping areas may be potentially associated with the olfactory pathway and nervous system. A racing performance QTL was detected in a CNVR of Thoroughbreds, Jeju riding horses, and Hanla horses, and the CNVR value was mixed for three breeds.
Efficacy and Safety of 4.7 mg Deslorelin Acetate Implants in Suppressing Oestrus Cycle in Prepubertal Female Dogs
Authors: Gontier Aymeric , Youala Myriam , Fontaine Christelle , Raibon Elsa , Fournel Sandrine , Briantais Philippe , Rigaut Delphine
Published: December 2022

Our multicentric, masked, controlled and randomised study aimed to assess the efficacy and safety of Suprelorin® 4.7 mg (Virbac, Carros, France) regarding oestrus prevention in prepubertal intact bitches. Twelve- to eighteen-week-old females (n = 83) were allocated either a deslorelin implant (n = 62) or 0.9% sodium chloride (n = 21) group. Clinical assessment (heat signs), 17β oestradiol and progesterone assays, and vaginal cytology were performed at day (D)0, D7, D21, month (M)3 and M6 after product administration, and were then performed every other month until reaching puberty. Trained owners assessed heat signs between each veterinary visit. All bitches (n = 83) reached puberty before M30. Deslorelin significantly extended the median time to sexual maturity when compared to the control group (377 days versus 217 days after D0, p < 0.0001). Three females, implanted between 16 and 18 weeks of age, expressed an induced oestrus. Additional descriptive data, collected over a 24 month-period, showed functional reproductive abilities in both deslorelin (n = 52) and control (n = 21) groups once puberty was achieved. In conclusion, Suprelorin® 4.7 mg seems to be an effective and safe option for postponing the onset of oestrus when administered to prepubertal female dogs aged from 12 to 16 weeks.
Reconstruction of the Major Maternal and Paternal Lineages in the Feral New Zealand Kaimanawa Horses
Authors: Sharif Muhammad Bilal , Fitak Robert Rodgers , Wallner Barbara , Orozco-terWengel Pablo , Frewin Simone , Fremaux Michelle , Mohandesan Elmira
Published: December 2022

New Zealand has the fourth largest feral horse population in the world. The Kaimanawas (KHs) are feral horses descended from various domestic horse breeds released into the Kaimanawa ranges in the 19th and 20th centuries. Over time, the population size has fluctuated dramatically due to hunting, large-scale farming and forestry. Currently, the herd is managed by an annual round-up, limiting the number to 300 individuals to protect the native ecosystem. Here, we genotyped 96 KHs for uniparental markers (mitochondrial DNA, Y-chromosome) and assessed their genetic similarity with respect to other domestic horses. We show that at least six maternal and six paternal lineages contributed unequally to the KH gene pool, and today’s KH population possibly represents two sub-populations. Our results indicate that three horse breeds, namely Welsh ponies, Thoroughbreds and Arabian horses had a major influence in the genetic-makeup of the extant KH population. We show that mitochondrial genetic diversity in KHs (π = 0.00687 ± 0.00355) is closer to that of the Sable Island horses (π = 0.0034 ± 0.00301), and less than other feral horse populations around the world. Our current findings, combined with ongoing genomic research, will provide insight into the population-specific genetic variation and inbreeding among KHs. This will largely advance equine research and improve the management of future breeding programs of these treasured New Zealand horse.
Posteriors in Limited Time
Authors: Bhattacharya Ayan
Published: December 2022

This paper obtains a measure-theoretic restriction that must be satisfied by a prior probability measure for posteriors to be computed in limited time. Specifically, it is shown that the prior must be factorizable. Factorizability is a set of independence conditions for events in the sample space that allows agents to calculate posteriors using only a subset of the dataset. The result has important implications for models in mathematical economics and finance that rely on a common prior. If one introduces the limited time restriction to Aumann’s famous Agreeing to Disagree setup, one sees that checking for factorizability requires agents to have access to every event in the measure space, thus severely limiting the scope of the agreement result.
Application of the FDEM Based on the CZM in Simulating Three-Point Bending Test of Frozen Soil
Authors: Wang Yongtao , Ma Baicong , Hua Weihang , Wang Wei , Ma Luxing , Wang Boyuan , Mei Zijian
Published: December 2022

The combined finite–discrete element method (FDEM) based on the cohesive zone model (the CZM) achieves cracking simulation by inserting cohesive elements between solid elements. In this study, three-point bending fracture tests of frozen soil were simulated by using the FDEM based on the CZM. Firstly, the sensitivity of the cohesive model parameters was analyzed. Secondly, through a series of simulations of the three-point bending test of frozen soil, it was found that the model with reasonable values of the CZM parameters had a good adaptability to the three-point bending cracking test of frozen soil, as the model not only reflects the load-displacement curve, but also has good correspondence with the fracture pattern compared with the test. Finally, the relationship between the CZM parameters and the specimens’ temperature under two loading rates of 1 mm/min and 0.1 mm/min was analyzed, and it was found that the CZM parameters had a good linear relationship with the specimens’ temperature. This paper is expected to provide a new possibility for the numerical simulation of frozen soil cracking.
Severe Socioeconomic Exposures Due to Enhanced Future Compound Flood-Heat Extreme Hazards in China
Authors: Li Haochuan , Gu Ziye , Chen Jie , Yin Jiabo , Gu Lei
Published: December 2022

As the climate warms, a new hazard, compound flood-heat extreme (CFH) events, characterized by the rapid succession of devastating floods and deadly heat (or vice-versa), are becoming increasingly frequent, threatening infrastructure and ecosystems. However, how this CFH hazard will change under future anthropogenic warming in China and their potential population and economic exposures remains unexamined. Here, we systematically quantify the projected changes in bivariate CHF hazards for 187 catchments in China during the 2071–2100 period relative to the 1985–2014 period and investigate the potential population and gross domestic product (GDP) exposure, by developing a climatic-hydrological-socioeconomic modelling chain. We find that there is a nationwide increase in CFH hazards and the historical 30-year CFH episodes are projected to increase by 10 times in southern catchments. Under the synergistic impacts of changing CFH episodes and population (GDP), a mass of people in southern (0.79–2.13 thousand/km2) and eastern (1.68 thousand/km2) catchments and an enormous sum of GDP in eastern catchments (400–912 million/km2) will be exposed to increasing CFH hazards. Our results highlight the necessity of improving both societal resilience and mitigation solutions to address such weather-related hazards.
3D Printed Voltammetric Sensor Modified with an Fe(III)-Cluster for the Enzyme-Free Determination of Glucose in Sweat
Authors: Koukouviti Eleni , Plessas Alexios K. , Economou Anastasios , Thomaidis Nikolaos , Papaefstathiou Giannis S. , Kokkinos Christos
Published: December 2022

In this work, a 3D printed sensor modified with a water-stable complex of Fe(III) basic benzoate is presented for the voltammetric detection of glucose (GLU) in acidic epidermal skin conditions. The GLU sensor was produced by the drop-casting of Fe(III)-cluster ethanolic mixture on the surface of a 3D printed electrode fabricated by a carbon black loaded polylactic acid filament. The oxidation of GLU was electrocatalyzed by Fe(III), which was electrochemically generated in-situ by the Fe(III)-cluster precursor. The GLU determination was carried out by differential pulse voltammetry without the interference from common electroactive metabolites presented in sweat (such as urea, uric acid, and lactic acid), offering a limit of detection of 4.3 μmol L−1. The exceptional electrochemical performance of [Fe3O(PhCO2)6(H2O)3]∙PhCO2 combined with 3D printing technology forms an innovative and low-cost enzyme-free sensor suitable for noninvasive applications, opening the way for integrated 3D printed wearable biodevices.
Anti-Correlated Myelin-Sensitive MRI Levels in Humans Consistent with a Subcortical to Sensorimotor Regulatory Process—Multi-Cohort Multi-Modal Evidence
Authors: Barnden Leighton , Crouch Benjamin , Kwiatek Richard , Shan Zack , Thapaliya Kiran , Staines Donald , Bhuta Sandeep , Del Fante Peter , Burnet Richard
Published: December 2022

Differential axonal myelination synchronises signalling over different axon lengths. The consequences of myelination processes described at the cellular level for the regulation of myelination at the macroscopic level are unknown. We analysed multiple cohorts of myelin-sensitive brain MRI. Our aim was to (i) confirm a previous report of anti-correlation between myelination in subcortical and sensorimotor areas in healthy subjects, (ii) and thereby test our hypothesis for a regulatory interaction between them. We analysed nine image-sets across three different human cohorts using six MRI modalities. Each image-set contained healthy controls (HC) and ME/CFS subjects. Subcortical and Sensorimotor regions of interest (ROI) were optimised for the detection of anti-correlations and the same ROIs were used to test the HC in all image-sets. For each cohort, median MRI values were computed in both regions for each subject and their correlation across the cohort was computed. We confirmed negative correlations in healthy controls between subcortical and sensorimotor regions in six image-sets: three T1wSE (p = 5 × 10−8, 5 × 10−7, 0.002), T2wSE (p =2 × 10−6), MTC (p = 0.01), and WM volume (p = 0.02). T1/T2 was the exception with a positive correlation (p = 0.01). This myelin regulation study is novel in several aspects: human subjects, cross-sectional design, ROI optimization, spin-echo MRI and reproducible across multiple independent image-sets. In multiple independent image-sets we confirmed an anti-correlation between subcortical and sensorimotor myelination which supports a previously unreported regulatory interaction. The subcortical region contained the brain’s primary regulatory nuclei. We suggest a mechanism has evolved whereby relatively low subcortical myelination in an individual is compensated by upregulated sensorimotor myelination to maintain adequate sensorimotor performance.
The Interrelated Multifactorial Actions of Cortisol and Klotho: Potential Implications in the Pathogenesis of Parkinson’s Disease
Authors: Luthra Nijee S. , Clow Angela , Corcos Daniel M.
Published: December 2022

The pathogenesis of Parkinson’s disease (PD) is complex, multilayered, and not fully understood, resulting in a lack of effective disease-modifying treatments for this prevalent neurodegenerative condition. Symptoms of PD are heterogenous, including motor impairment as well as non-motor symptoms such as depression, cognitive impairment, and circadian disruption. Aging and stress are important risk factors for PD, leading us to explore pathways that may either accelerate or protect against cellular aging and the detrimental effects of stress. Cortisol is a much-studied hormone that can disrupt mitochondrial function and increase oxidative stress and neuroinflammation, which are recognized as key underlying disease mechanisms in PD. The more recently discovered klotho protein, considered a general aging-suppressor, has a similarly wide range of actions but in the opposite direction to cortisol: promoting mitochondrial function while reducing oxidative stress and inflammation. Both hormones also converge on pathways of vitamin D metabolism and insulin resistance, also implicated to play a role in PD. Interestingly, aging, stress and PD associate with an increase in cortisol and decrease in klotho, while physical exercise and certain genetic variations lead to a decrease in cortisol response and increased klotho. Here, we review the interrelated opposite actions of cortisol and klotho in the pathogenesis of PD. Together they impact powerful and divergent mechanisms that may go on to influence PD-related symptoms. Better understanding of these hormones in PD would facilitate the design of effective interventions that can simultaneously impact the multiple systems involved in the pathogenesis of PD.
Primary Headaches and Physical Performance: A Professional Youth Female Soccer Team Study
Authors: Kobus Magdalena , Żądzińska Elżbieta , Michaelides Marcos , Parpa Koulla
Published: December 2022

The vast majority of the research on acute post-traumatic incidents in athletes focuses on migraines. Physical exercise might reduce the frequency of migraines as well as trigger a migraine attack. The objectives of the study were to evaluate primary headache prevalence in professional young female soccer players and to assess whether headaches are related to physical performance. To the best of our knowledge, this is the first study that has considered the relationship between primary headaches and physical performance assessment. The research was conducted in 19 females aged 12–17 from a professional youth soccer academy. Players completed a questionnaire about health status and then performed laboratory physical tests (e.g., cardiopulmonary tests, countermovement and squat jumps, handgrip, sit-and-reach tests). Subsequently, players were separated into a headache group and a headache-free control group. In the analysed group, eight female players (42%) suffered from primary headaches. Among the performance parameters, a significant result was found in terms of flexibility. Females from the headache group had higher results in the sit-and-reach test than females from the control group (p = 0.029). Flexibility as well as mobility in migraine patients is an area with significant potential for further investigation, as little research has been conducted to date.
The Role of Relative Deprivation and Attribution Style in the Relationship between Organizational Fairness and Employees’ Service Innovation Behavior
Authors: Li Zhao , Liang Wangbing , Bao Yinggang , Zhang Ruili
Published: December 2022

The basis of organizational innovation is employee innovation, which is of great significance for organizations to gain a competitive advantage. At present, the research on the influencing factors of employee service innovation behavior is increasing. This study, based on the social cognitive theory, with relative deprivation as the mediator and attribution style as the moderator, explores the mechanism of the effect of organizational fairness on employee service innovation behavior. Taking 342 employees of service-oriented enterprises as the subjects of investigation, this paper empirically tests the theoretical model by using Amos and SPSS. The results indicated the following, organizational fairness was positively related to employees’ service innovation behavior. Relative deprivation partially mediated the relationship between organizational fairness and service innovation behavior. An external attribution style positively moderated the relationship between relative deprivation and employee service innovation behavior. An external attribution style also positively moderated the mediation effect of the relationship between organizational fairness and service innovation behavior. The internal attribution style negatively moderated the relationship between relative deprivation and employee service innovation behavior. The internal attribution style also negatively moderated the mediation effect of the relationship between organizational fairness and service innovation behavior. The conclusion of this study has managerial implications on how to promote employee service innovation behavior in service-oriented enterprises.
An Experimental and Numerical Study on Fire Spread in a Furnished Room
Authors: Król Małgorzata , Król Aleksander
Published: December 2022

The main objective of this research was to examine the development of fire in a furnished room in a typical high-rise building. This work was part of research on the fire safety of building occupants. It included two controlled fires in which a standard sofa in a room was set on fire. Several thermocouple trees were arranged in the test room and the temperature was continuously recorded. Additionally, each fire test was videotaped for further analysis. Since an unexpected forepeak of the temperature course was observed, special attention was paid to explain this phenomenon. For this purpose, numerical models of fire development in a furnished room were built using the well-recognized software package, ANSYS Fluent and Fire Dynamic Simulator (FDS). The numerical research was focused on fire spread over a single piece of furniture, the sofa. The data recorded in real experiments were used to tune and validate the numerical models. The results of the Fluent numerical simulation were consistent with the recorded experimental data and proved that after the initial growth, there was a critical phase of fire development in which the fire might almost snuff or flare again. Meanwhile, the FDS results, despite being generally in accordance with the experiment, did not reproduce this critical phase of fire spreading.