bluuebunny/crossref_metadata_2025 huggingface dataset:
Functionality and Storability of Cookies Fortified at the Industrial Scale with up to 75% of Apple Pomace Flour Produced by Dehydration
Authors: Zlatanović Snežana , Kalušević Ana , Micić Darko , Laličić-Petronijević Jovanka , Tomić Nikola , Ostojić Sanja , Gorjanović Stanislava
Published: November 2019

Apple pomace flour (APF) with high content of dietary fibers (DF), total polyphenolics (TPCs) and flavonoids (TFCs) was produced at the industrial scale. Bulk and tapped density, swelling, water and oil holding capacity, solubility and hydration density of fine and coarse APF with average particle size 0.16 and 0.50 mm were compared. The effect of wheat flour substitution with 25%, 50% and 75% of fine and coarse APF was studied upon cookies production at the industrial scale and after one year of storage. Coarse APF performed better in respect to sensorial properties, content and retention of dietary compounds and antioxidant (AO) activity. The cookies with optimal share of coarse APF (50%) contained 21 g/100 g of DF and several times higher TPC, TFC as well as AO activity than control cookies, retained well health promoting compounds and maintained an intensely fruity aroma and crispy texture. They were acceptable for consumers according to the hedonic test.
Multi-Point and Anti-Periodic Conditions for Generalized Langevin Equation with Two Fractional Orders
Authors: Salem Ahmed , Alghamdi Balqees
Published: November 2019

With anti-periodic and a new class of multi-point boundary conditions, we investigate, in this paper, the existence and uniqueness of solutions for the Langevin equation that has Caputo fractional derivatives of two different orders. Existence of solutions is obtained by applying Krasnoselskii–Zabreiko’s and the Leray–Schauder fixed point theorems. The Banach contraction mapping principle is used to investigate the uniqueness. Illustrative examples are provided to apply of the fundamental investigations.
Massive Stars in the Tarantula Nebula: A Rosetta Stone for Extragalactic Supergiant HII Regions
Authors: Crowther Paul A.
Published: November 2019

A review of the properties of the Tarantula Nebula (30 Doradus) in the Large Magellanic Cloud is presented, primarily from the perspective of its massive star content. The proximity of the Tarantula and its accessibility to X-ray through radio observations permit it to serve as a Rosetta Stone amongst extragalactic supergiant HII regions since one can consider both its integrated characteristics and the individual properties of individual massive stars. Recent surveys of its high mass stellar content, notably the VLT FLAMES Tarantula Survey (VFTS), are reviewed, together with VLT/MUSE observations of the central ionizing region NGC 2070 and HST/STIS spectroscopy of the young dense cluster R136, provide a near complete Hertzsprung-Russell diagram of the region, and cumulative ionizing output. Several high mass binaries are highlighted, some of which have been identified from a recent X-ray survey. Brief comparisons with the stellar content of giant HII regions in the Milky Way (NGC 3372) and Small Magellanic Cloud (NGC 346) are also made, together with Green Pea galaxies and star forming knots in high-z galaxies. Finally, the prospect of studying massive stars in metal poor galaxies is evaluated.
Differential Expression of Genes Related to Sexual Determination Can Modify the Reproductive Cycle of Astyanax scabripinnis (Characiformes: Characidae) in B Chromosome Carrier Individuals
Authors: Castro Jonathan Pena , Hattori Ricardo Shohei , Yoshinaga Túlio Teruo , Silva Duílio Mazzoni Zerbinato de Andrade , Ruiz-Ruano Francisco J. , Foresti Fausto , Santos Mateus Henrique , Almeida Mara Cristina de , Moreira-Filho Orlando , Artoni Roberto Ferreira
Published: November 2019

The species complex Astyanax scabripinnis is one of the most studied with respect to origin, distribution, and frequency of B chromosomes, and is considered a model organism for evolutionary studies. Research using population inferences about the occurrence and frequency of the B chromosome shows seasonal variation between sexes, which is associated with the presence of this supernumerary element. We hypothesized that the B chromosome could influence the sex ratio of these animals. Based on this assumption, the present work aimed to investigate if differences exist among levels of gene expression with qRT-PCR of the amh (associated with testicular differentiation) and foxl2a (associated with ovarian differentiation) genes between B-carrier and non-B-carrier individuals. The results showed that for the amh gene, the difference in expression between animals with B chromosomes was not accentuated compared to that in animals without this chromosome. Expression of foxl2a in B-carrier females, however, was reduced by 73.56% compared to females that lacked the B chromosome. Males had no difference in expression of the amh and foxl2a genes between carriers and non-carriers of the B chromosome. Results indicate that the presence of B chromosomes is correlated with the differential expression of sex-associated genes. An analysis of these results integrated with data from other studies on the reproductive cycle in the same species reveals that this difference in expression may be expanding the reproductive cycle of the species.
Genome-Wide Analysis of Basic Helix–Loop–Helix Superfamily Members Reveals Organization and Chilling-Responsive Patterns in Cabbage (Brassica oleracea var. capitata L.)
Authors: Shan Xi , Zhang Wei , Yu Fangwei , Wang Shenyun , Li Jianbin , Tang Jun , Dai Zhongliang
Published: November 2019

Basic helix–loop–helix (bHLH) transcription factor (TF) family is commonly found in eukaryotes, which is one of the largest families of regulator proteins. It plays an important role in plant growth and development, as well as various biotic and abiotic stresses. However, a comprehensive analysis of the bHLH family has not been reported in Brassica oleracea. In this study, we systematically describe the BobHLHs in the phylogenetic relationships, expression patterns in different organs/tissues, and in response to chilling stress, and gene and protein characteristics. A total of 234 BobHLH genes were identified in the B. oleracea genome and were further clustered into twenty-three subfamilies based on the phylogenetic analyses. A large number of BobHLH genes were unevenly located on nine chromosomes of B. oleracea. Analysis of RNA-Seq expression profiles revealed that 21 BobHLH genes exhibited organ/tissue-specific expression. Additionally, the expression of six BobHLHs (BobHLH003, -048, -059, -093, -109, and -148) were significantly down-regulated in chilling-sensitive cabbage (CS-D9) and chilling-tolerant cabbage (CT-923). At 24h chilling stress, BobHLH054 was significantly down-regulated and up-regulated in chilling-treated CS-D9 and CT-923. Conserved motif characterization and exon/intron structural patterns showed that BobHLH genes had similar structures in the same subfamily. This study provides a comprehensive analysis of BobHLH genes and reveals several candidate genes involved in chilling tolerance of B. oleracea, which may be helpful to clarify the roles of bHLH family members and understand the regulatory mechanisms of BobHLH genes in response to the chilling stress of cabbage.
Simulation and Ergonomic Evaluation of Welders’ Standing Posture Using Jack Software
Authors: Zhang Yongbao , Wu Xiang , Gao Jingqi , Chen Jianwu , Xv Xun
Published: November 2019

Ergonomics research strives to make workers’ labor more efficient, safer, and more comfortable. Therefore, six digital humans and welding torch model were built and evaluated based on the Jack software in order to improve the ergonomics of welders’ standing postures. Three sets of standing welding actions were designed: walking, raising arm, and contracting arm. Through the Lower Back Analysis, Ovako Working Posture Analysis, Comfort Assessment, and Rapid Upper Limb Assessment, this paper evaluated the optimum range of the weight of the welding torch, the upper limb posture, and the neck posture of the welder. Firstly, the results show that Chinese welders should not use a welding torch with a weight of more than 6 kg when standing up. Secondly, for adult males in the 5th, 50th, 95th percentile of body size, the best operating distance is 321 mm, 371 mm, and 421 mm, respectively, and the best operating height is 1050 mm, 1100 mm, and 1150 mm, respectively; for females in the same percentiles, the optimal operating distance is 271 mm, 321 mm, and 371 mm, respectively, and the optimal operating height is 1000 mm, 1050 mm, and 1100 mm, respectively. Moreover, the horizontal and vertical rotation angle of the welder’s neck should not exceed 15° and 8.7°. The adjustment strategy not only has a positive effect on improving welders’ operational posture and preventing fatigue and injury to the welder, but it also develops research ideas for promoting safety from the perspective of ergonomics.
The Impact of Liquor Restrictions on Serious Assaults across Queensland, Australia
Authors: Taylor Nicholas , Coomber Kerri , Mayshak Richelle , Zahnow Renee , Ferris Jason , Miller Peter
Published: November 2019

Aims: This study aimed to explore the relationship between a 00:00 liquor restriction, introduced on 1 July 2016, and alcohol-related harm by examining its impact on serious assault numbers during high-alcohol hours (20:00–6:00 Friday and Saturday night), from 1 January 2009 to 30 June 2018. Methods: Two types of locations only impacted by the liquor restriction were identified: designated safe night precincts (SNPs) and other local government areas (LGAs). A times series autoregressive integrated moving average analysis was used to estimate the influence of liquor restrictions on police-recorded serious assaults in the two years following the policy introduction, for SNPs and LGAs separately. Results: Contrarily to our predictions, monthly police-recorded serious assaults did not significantly change within SNPs or LGAs following the introduction of liquor restrictions. Conclusion: The implementation of the Queensland liquor restriction did not result in a clear, unique reduction in serious assault trends. Further investigation should consider the impact of liquor restrictions in conjunction with other policy changes as public perception of restrictions and their cumulative impact may produce varied outcomes.
Application of Monitoring Network Design and Feedback Information for Adaptive Management of Coastal Groundwater Resources
Authors: Lal Alvin , Datta Bithin
Published: November 2019

Optimal strategies for the management of coastal groundwater resources can be derived using coupled simulation-optimization based management models. However, the management strategy actually implemented on the field sometimes deviates from the recommended optimal strategy, resulting in field-level deviations. Monitoring these field-level deviations during actual implementation of the recommended optimal management strategy and sequentially updating the management model using the feedback information is an important step towards efficient adaptive management of coastal groundwater resources. In this study, a three-phase adaptive management framework for a coastal aquifer subjected to saltwater intrusion is applied and evaluated for a regional-scale coastal aquifer study area. The methodology adopted includes three sequential components. First, an optimal management strategy (consisting of groundwater extraction from production and barrier wells) is derived and implemented for optimal management of the aquifer. The implemented management strategy is obtained by solving a homogenous ensemble-based coupled simulation-optimization model. Second, a regional-scale optimal monitoring network is designed for the aquifer system considering possible user noncompliance of a recommended management strategy, and uncertainties in estimating aquifer parameters. A new monitoring network design objective function is formulated to ensure that candidate monitoring wells are placed in high risk (highly contaminated) locations. In addition, a new methodology is utilized to select candidate monitoring wells in areas representative of the entire model domain. Finally, feedback information in the form of measured concentrations obtained from the designed optimal monitoring wells is used to sequentially modify pumping strategies for future time periods in the management horizon. The developed adaptive management framework is evaluated by applying it to the Bonriki aquifer system located in Kiribati, which is a small developing island country in the South Pacific region. Overall, the results from this study suggest that the implemented adaptive management strategy has the potential to address important practical implementation issues arising due to noncompliance of an optimal management strategy and uncertain aquifer parameters.
HLA-F Allele-Specific Peptide Restriction Represents an Exceptional Proteomic Footprint
Authors: Hò Gia-Gia T. , Heinen Funmilola J. , Blasczyk Rainer , Pich Andreas , Bade-Doeding Christina
Published: November 2019

Peptide-dependent engagement between human leucocyte antigens class I (HLA-I) molecules and their cognate receptors has been extensively analyzed. HLA-F belongs to the non-classical HLA-Ib molecules with marginal polymorphic nature and tissue restricted distribution. The three common allelic variants HLA-F*01:01/01:03/01:04 are distinguished by polymorphism outside the peptide binding pockets (residue 50, α1 or residue 251, α3) and are therefore not considered relevant for attention. However, peptide selection and presentation undergoes a most elaborated extraction from the whole available proteome. It is known that HLA-F confers a beneficial effect on disease outcome during HIV-1 infections. The interaction with the NK cell receptor initiates an antiviral downstream immune response and lead to delayed disease progression. During the time of HIV infection, HLA-F expression is upregulated, while its interaction with KIR3DS1 is diminished. The non-polymorphic nature of HLA-F facilitates the conclusion that understanding HLA-F peptide selection and presentation is essential to a comprehensive understanding of this dynamic immune response. Utilizing soluble HLA technology we recovered stable pHLA-F*01:01, 01:03 and 01:04 complexes from K562 cells and analyzed the peptides presented. Utilizing a sophisticated LC-MS-method, we analyzed the complete K562 proteome and matched the peptides presented by the respective HLA-F subtypes with detected proteins. All peptides featured a length of 8 to 24 amino acids and are not N-terminally anchored; the C-terminus is preferably anchored by Lys. To comprehend the alteration of the pHLA-F surface we structurally compared HLA-F variants bound to selected peptides. The peptides were selected from the same cellular content; however, no overlap between the proteomic source of F*01:01, 01:03 or 01:04 selected peptides could be observed. Recognizing the balance between HLA-F expression, HLA-F polymorphism and peptide selection will support to understand the role of HLA-F in viral pathogenesis.
Identification of the Virulence Factors of Candidatus Liberibacter asiaticus via Heterologous Expression in Nicotiana benthamiana using Tobacco Mosaic Virus
Authors: Ying Xiaobao , Wan Mengyuan , Hu Linshuang , Zhang Jinghua , Li Hui , Lv Dianqiu
Published: November 2019

Huanglongbing (HLB), also known as citrus greening, is the most destructive disease of citrus worldwide. HLB is associated with the non-culturable bacterium, Candidatus Liberibacter asiaticus (CaLas) in the United States. The virulence mechanism of CaLas is largely unknown, partly because of the lack of a mutant library. In this study, Tobacco mosaic virus (TMV) and Nicotiana benthamiana (N. benthamiana) were used for large-scale screening of the virulence factors of CaLas. Agroinfiltration of 60 putative virulence factors in N. benthamiana led to the identification of four candidates that caused severe symptoms in N. benthamiana, such as growth inhibition and cell death. CLIBASIA_05150 and CLIBASIA_04065C (C-terminal of CLIBASIA_04065) could cause cell death in the infiltrated leaves at five days post infiltration. Two low-molecular-weight candidates, CLIBASIA_00470 and CLIBASIA_04025, could inhibit plant growth. By converting start codon to stop codon or frameshifting, the four genes lost their harmful effects to N. benthamiana. It indicated that the four virulence factors functioned at the protein level rather than at the RNA level. The subcellular localization of the four candidates was determined by confocal laser scanning microscope. CLIBASIA_05150 located in the Golgi apparatus; CLIBASIA_04065 located in the mitochondrion; CLIBASIA_00470 and CLIBASIA_04025 distributed in cells as free GFP. The host proteins interacting with the four virulence factors were identified by yeast two-hybrid. The host proteins interacting with CLIBASIA_00470 and CLIBASIA_04025 were overlapping. Based on the phenotypes, the subcellular localization and the host proteins identified by yeast two-hybrid, CLIBASIA_00470 and CLIBASIA_04025, functioned redundantly. The hypothesis of CaLas virulence was proposed. CaLas affects citrus development and suppresses citrus disease resistance, comprehensively, in a complicated manner. Ubiquitin-mediated protein degradation might play a vital role in CaLas virulence. Deep characterization of the interactions between the identified virulence factors and their prey will shed light on HLB. Eventually, it will help in developing HLB-resistant citrus and save the endangered citrus industry worldwide.
N-Dihydrogalactochitosan Potentiates the Radiosensitivity of Liver Metastatic Tumor Cells Originated from Murine Breast Tumors
Authors: Wang Chung-Yih , Chang Chun-Yuan , Wang Chun-Yu , Liu Kaili , Kang Chia-Yun , Lee Yi-Jang , Chen Wei R.
Published: November 2019

Radiation is a widely used therapeutic method for treating breast cancer. N-dihydrogalactochitosan (GC), a biocompatible immunostimulant, is known to enhance the effects of various treatment modalities in different tumor types. However, whether GC can enhance the radiosensitivity of cancer cells remains to be explored. In this study, triple-negative murine 4T1 breast cancer cells transduced with multi-reporter genes were implanted in immunocompetent Balb/C mice to track, dissect, and identify liver-metastatic 4T1 cells. These cells expressed cancer stem cell (CSC) -related characteristics, including the ability to form spheroids, the expression of the CD44 marker, and the increase of protein stability. We then ex vivo investigated the potential effect of GC on the radiosensitivity of the liver-metastatic 4T1 breast cancer cells and compared the results to those of parental 4T1 cells subjected to the same treatment. The cells were irradiated with increased doses of X-rays with or without GC treatment. Colony formation assays were then performed to determine the survival fractions and radiosensitivity of these cells. We found that GC preferably increased the radiosensitivity of liver-metastatic 4T1 breast cancer cells rather than that of the parental cells. Additionally, the single-cell DNA electrophoresis assay (SCDEA) and γ-H2AX foci assay were performed to assess the level of double-stranded DNA breaks (DSBs). Compared to the parental cells, DNA damage was significantly increased in liver-metastatic 4T1 cells after they were treated with GC plus radiation. Further studies on apoptosis showed that this combination treatment increased the sub-G1 population of cells, but not caspase-3 cleavage, in liver-metastatic breast cancer cells. Taken together, the current data suggest that the synergistic effects of GC and irradiation might be used to enhance the efficacy of radiotherapy in treating metastatic tumors.
Neutrophil Maturation and Survival Is Controlled by IFN-Dependent Regulation of NAMPT Signaling
Authors: Siakaeva Elena , Pylaeva Ekaterina , Spyra Ilona , Bordbari Sharareh , Höing Benedikt , Kürten Cornelius , Lang Stephan , Jablonska Jadwiga
Published: November 2019

Granulocyte-colony stimulating factor (G-CSF)/nicotinamide phosphoribosyltransferase (NAMPT) signaling has been shown to be crucial for the modulation of neutrophil development and functionality. As this signaling pathway is significantly suppressed by type I interferons (IFNs), we aimed to study how the regulation of neutrophil differentiation and phenotype is altered in IFN-deficient mice during granulopoiesis. The composition of bone marrow granulocyte progenitors and their Nampt expression were assessed in bone marrow of type I IFN receptor knockout (Ifnar1-/-) mice and compared to wild-type animals. The impact of NAMPT inhibition on the proliferation, survival, and differentiation of murine bone marrow progenitors, as well as of murine 32D and human HL-60 neutrophil-like cell lines, was estimated. The progressive increase of Nampt expression during neutrophil progenitor maturation could be observed, and it was more prominent in IFN-deficient animals. Altered composition of bone marrow progenitors in these mice correlated with the dysregulation of apoptosis and altered differentiation of these cells. We observed that NAMPT is vitally important for survival of early progenitors, while at later stages it delays the differentiation of neutrophils, with moderate effect on their survival. This study shows that IFN-deficiency leads to the elevated NAMPT expression in the bone marrow, which in turn modulates neutrophil development and differentiation, even in the absence of tumor-derived stimuli.
A Gas Turbine Cooled-Stage Expansion Model for the Simulation of Blade Cooling Effects on Cycle Performance
Authors: Masci Roberta , Sciubba Enrico
Published: November 2019

Modern gas turbine firing temperatures (1500–2000 K) are well beyond the maximum allowable blade material temperatures. Continuous safe operation is made possible by cooling the HP turbine first stages, nozzle vanes and rotor blades, with a portion of the compressor discharge air, a practice that induces a penalty on the thermal efficiency cycle. Therefore, a current issue is to investigate the real advantage, technical and economical, of raising maximum temperatures much further beyond current values. In this paper, process simulations of a gas turbine are performed to assess HP turbine first-stage cooling effects on cycle performance. A new simplified and properly streamlined model is proposed for the non-adiabatic expansion of the hot gas mixed with the cooling air within the blade passage, which allows for a comparison of several cycle configurations at different turbine inlet temperatures (TIT) and total turbine expansion ratio (PR) with a realistically acceptable degree of approximation. The calculations suggest that, at a given PR, the TIT can be increased in order to reach a higher cycle efficiency up to a limit imposed by the required amount and temperature of the cooling air. Beyond this limit, no significant gains in thermal efficiency are obtained by adopting higher PR and/or increasing the TIT, so that it is convenient in terms of cycle performance to design at a lower rather than higher PR. The small penalty on cycle efficiency is compensated by the lower plant cost. The results of our model agree with those of some previous and much more complex and computationally expensive studies, so that the novelty of this paper lies in the original method adopted on which the proposed model is based, and in the fast, accurate, and low resource intensity of the corresponding numerical procedure, all advantages that can be crucial for industry needs. The presented analysis is purely thermodynamic and it includes no investigation on the effects of the different configurations on plant costs. Therefore, performing a thermo-economic analysis of the air-cooled gas turbine power plant is the next logical step.
The Impact of Neighbourhood Deprivation on Embryonic Growth Trajectories: Rotterdam Periconception Cohort
Authors: Gootjes Dionne V. , Koster Maria P. H. , Willemsen Sten P. , Koning Anton H. J. , Steegers Eric A. P. , Steegers-Theunissen Régine P. M.
Published: November 2019

Background: Neighbourhood deprivation is a risk factor for impaired health and adverse pregnancy outcomes. We investigated whether living in a deprived neighbourhood is associated with embryonic growth. Methods: From the Predict cohort, we studied 566 women who underwent repeated first trimester ultrasound examinations. Crown rump length (CRL; n = 1707) and embryonic volume (EV; n = 1462) were measured using three-dimensional techniques. Neighbourhood deprivation was assessed using the neighbourhood status scores (NSS) of the Dutch Social Cultural Planning office. A high NSS represents a non-deprived neighbourhood. Associations between the NSS and embryonic growth were investigated using linear mixed models. Adjustment was performed for individual-level factors: maternal age, geographic origin, educational level, BMI, folic acid supplement use, fruit and vegetable intake, alcohol use and smoking habits. Results: The NSS was negatively associated with embryonic growth: a higher score (a less deprived neighbourhood) was associated with a smaller CRL and EV; adjusted β: −0.025 (95% CI −0.046, −0.003) and adjusted β: −0.015 (95% CI −0.026, −0.003). At 11 weeks of pregnancy, we observed a 0.55 cm3 smaller EV (7.65 cm3 vs. 7.10 cm3) and 1.08 mm smaller CRL (43.14 mm vs. 42.06 mm) in the highest compared to the lowest category. Conclusion: In deprived neighbourhoods, embryos are larger than in non-deprived neighbourhoods.
Correlations between Molecular Landscape and Sonographic Image of Different Variants of Papillary Thyroid Carcinoma
Authors: Lewiński Andrzej , Adamczewski Zbigniew , Zygmunt Arkadiusz , Markuszewski Leszek , Karbownik-Lewińska Małgorzata , Stasiak Magdalena
Published: November 2019

Papillary thyroid carcinoma (PTC), the most common thyroid cancer, is predominantly driven by mutations in BRAF (primarily p. V600E) and RAS oncogenes. Ultrasound (US) examination provides significant diagnostic data in the management of thyroid nodules, as many sonographic features of thyroid lesions are correlated with the potential risk of thyroid carcinoma. The aim of the study was to analyze the current literature in regard to the potential associations between genetic landscape and sonographic features of PTC. Based on the current literature, sonographic features of PTCs correlate with their molecular drivers, particularly between tumors harboring BRAFV600E versus activating RAS mutations, although many of these findings appear to be dependent on the tumor variant. Suspicious US findings, such as hypoechogenicity, spiculated/microlobulated margins, non-parallel orientation/taller-than-wide shape, and the presence of microcalcifications, are typical for PTC positive for BRAFV600E mutations. On the contrary, tumors with RAS mutations are most frequently hypo- or isoechoic and ovoid-to-round in shape, with smooth margins and without calcifications. There are also some US features typical for PTCs harboring other mutations, including BRAFK601E, RET/PTC rearrangements, PAX8-PPARγ, CTNNB1, and APC. However, further research is necessary, as some rare PTC variants still cannot be reliably analyzed due to the scarce published data.
Post-Concussion Symptoms in Complicated vs. Uncomplicated Mild Traumatic Brain Injury Patients at Three and Six Months Post-Injury: Results from the CENTER-TBI Study
Authors: Voormolen Daphne C. , Haagsma Juanita A. , Polinder Suzanne , Maas Andrew I.R. , Steyerberg Ewout W. , Vuleković Petar , Sewalt Charlie A. , Gravesteijn Benjamin Y. , Covic Amra , Andelic Nada , Plass Anne Marie , von Steinbuechel Nicole ,   on behalf of the CENTER-TBI Participants and Investigators
Published: November 2019

The aim of this study was to assess the occurrence of post-concussion symptoms and post-concussion syndrome (PCS) in a large cohort of patients after complicated and uncomplicated mild traumatic brain injury (mTBI) at three and six months post-injury. Patients were included through the prospective cohort study: Collaborative European NeuroTrauma Effectiveness Research (CENTER-TBI). Patients enrolled with mTBI (Glasgow Coma Scale 13–15) were further differentiated into complicated and uncomplicated mTBI based on the presence or absence of computed tomography abnormalities, respectively. The Rivermead Post-Concussion Symptoms Questionnaire (RPQ) assessed post-concussion symptoms and PCS according to the mapped ICD-10 classification method. The occurrence of post-concussion symptoms and syndrome at both time points was calculated. Chi square tests were used to test for differences between and within groups. Logistic regression was performed to analyse the association between complicated versus uncomplicated mTBI and the prevalence of PCS. Patients after complicated mTBI reported slightly more post-concussion symptoms compared to those after uncomplicated mTBI. A higher percentage of patients after complicated mTBI were classified as having PCS at three (complicated: 46% vs. uncomplicated: 35%) and six months (complicated: 43% vs. uncomplicated 34%). After adjusting for baseline covariates, the effect of complicated versus uncomplicated mTBI at three months appeared minimal: odds ratio 1.25 (95% confidence interval: 0.95–1.66). Although patients after complicated mTBI report slightly more post-concussion symptoms and show higher PCS rates compared to those after uncomplicated mTBI at three and six months, complicated mTBI was only found a weak indicator for these problems.
Perinatal Outcome and Long-Term Gastrointestinal Morbidity of Offspring of Women with Celiac Disease
Authors: Abecassis Avishag , Wainstock Tamar , Sheiner Eyal , Pariente Gali
Published: November 2019

The aim of this study was to evaluate perinatal outcome and long-term offspring gastrointestinal morbidity of women with celiac disease. Perinatal outcomes, as well as long-term gastrointestinal morbidity of offspring of mothers with and without celiac disease were assessed. The study groups were followed until 18 years of age for gastrointestinal-related morbidity. For perinatal outcomes, generalized estimation equation (GEE) models were used. A Kaplan–Meier survival curve was used to compare cumulative incidence of long-term gastrointestinal morbidity, and Cox proportional hazards models were constructed to control for confounders. During the study period, 243,682 deliveries met the inclusion criteria, of which 212 (0.08%) were to mothers with celiac disease. Using GEE models, maternal celiac disease was noted as an independent risk factor for low birth weight and cesarean delivery. Offspring born to mothers with celiac disease had higher rates of gastrointestinal related morbidity (Kaplan–Meier log rank test P < 0.001). Using a Cox proportional hazards model, being born to a mother with celiac disease was found to be an independent risk factor for long-term gastrointestinal morbidity of the offspring. Pregnancy of women with celiac disease is independently associated with adverse perinatal outcome as well as higher risk for long-term gastrointestinal morbidity of offspring.
Composite Electrodes of Activated Carbon and Multiwall Carbon Nanotubes Decorated with Silver Nanoparticles for High Power Energy Storage
Authors: Markoulidis Foivos , Todorova Nadia , Grilli Rossana , Lekakou Constantina , Trapalis Christos
Published: November 2019

Composite materials in electrodes for energy storage devices can combine different materials of high energy density, in terms of high specific surface area and pseudocapacitance, with materials of high power density, in terms of high electrical conductivity and features lowering the contact resistance between electrode and current collector. The present study investigates composite coatings as electrodes for supercapacitors with organic electrolyte 1.5 M TEABF4 in acetonitrile. The composite coatings contain high surface area activated carbon (AC) with only 0.15 wt% multiwall carbon nanotubes (MWCNTs) which, dispersed to their percolation limit, offer high conductivity. The focus of the investigations is on the decoration of MWCNTs with silver nanoparticles, where smaller Ag crystallites of 16.7 nm grew on carboxylic group-functionalized MWCNTs, MWCNT–COOH, against 27–32 nm Ag crystallites grown on unfunctionalized MWCNTs. All Ag-decorated MWCNTs eliminate the contact resistance between the composite electrode and the current collector that exists when undecorated MWCNTs are used in the composite electrodes. Ag-decorated MWCNT–COOH tripled the power density and Ag-decorated MWCNT additive doubled the power density and increased the maximum energy density by 6%, due to pseudocapacitance of Ag, compared to composite electrodes with undecorated MWCNTs.
Comparative Analysis of Linear and Non-Linear Programming Techniques for the Optimization of Ship Machinery Systems
Authors: Baldasso Enrico , Elg Mia , Haglind Fredrik , Baldi Francesco
Published: November 2019

The selection of a proper machinery system is one of the primary decisions to be taken during the ship design phase. Nonetheless, this selection is made challenging by the presence of a variety of alternatives, and by the limited data availability at the early stages of the design phase. An optimization framework is presented in this paper, supporting decision making at the earliest stages of the ship design process. The framework is suitable to perform the screening and the selection of optimal machinery configurations for a predefined ship operational profile, and it includes both linear and non-linear optimization routines. The results of the linear and the non-linear approaches are compared, and indications on what conditions are the most suitable for the application of one or the other approach are provided. Both approaches are tested for two case studies, a bulk carrier and a small cruise ship. The results indicate that both optimization approaches lead to the same layout of the machinery system, but to slightly different unit scheduling. This suggests that the use of the linear approach is suitable for design purposes, but less appropriate for operational optimization. In addition, the findings of the work suggest that the trade-off between fuel consumption and volume of the engines should be considered when selecting the machinery system for a ship.
Cultural Expertise: An Emergent Concept and Evolving Practices
Authors: Holden Livia
Published: November 2019

This introduction provides a snapshot on cultural expertise as an emergent concept in the socio-legal studies and evolving practices in the formulation of rights and the resolution of conflicts in and out of court. It starts with the definition of cultural expertise and the need for an integrated and broad conceptualization that includes all the arrays of socio-legal instruments that use knowledge from the social sciences to assist decision-making authorities in the settlement of conflicts. It then mentions the wide span of fields of cultural expertise going from the recognition of the rights of autochthone minorities and the First Nations to the politics of cultural expertise in modern reformulations of customs, vis-à-vis gender rights, including the revisitation of socio-legal instruments such as the cultural test and the scrutiny of psychiatric evaluation in criminal trials. It concludes by offering short descriptions of the papers included in the Special Issue, which include judicial practices involving cultural experts and surveys of the most frequent fields of expert witnessing that are related to the culture. In addition, it interrogates who the experts are; how cultural expert witnessing has been received; how cultural expertise has developed across the sister disciplines; and finally, it asks whether academic truth and legal truth are commensurable.
Morphological and Spectroscopic Study of an Apatite Layer Induced by Fast-Set Versus Regular-Set EndoSequence Root Repair Materials
Authors: Abu Zeid Sawsan T. , Alamoudi Ruaa A. , Abou Neel Ensanya A. , Mokeem Saleh Abeer A.
Published: November 2019

This study aimed to evaluate the morphology and chemistry of an apatite layer induced by fast-set versus regular-set EndoSequence root repair materials using spectroscopic analysis. Holes of a 4 mm diameter were created in the root canal dentin, which were filled with the test material. Fetal calf serum was used as the incubation medium, and the samples incubated in deionized water were used as controls. The material-surface and material-dentin interfaces were analyzed after 28 days using Raman and infrared spectroscopy, scanning electron microscopy/energy dispersive X-ray, and X-ray diffraction. After incubation in fetal calf serum, both materials formed a uniform layer of calcium phosphate precipitate on their surfaces, with the dentinal interface. This precipitated layer was a combination of hydroxyapatite and calcite or aragonite, and had a high mineral maturity with the regular-set paste. However, its crystallinity index was high with the fast-set putty. Typically, both consistencies (putty and paste) of root repair material have an apatite formation ability when they are incubated in fetal calf serum. This property could be beneficial in improving their sealing ability for root canal dentin.
Shrinkage and Mitigation Strategies to Improve the Dimensional Stability of CaO-FeOx-Al2O3-SiO2 Inorganic Polymers
Authors: Ascensão Guilherme , Beersaerts Glenn , Marchi Maurizio , Segata Monica , Faleschini Flora , Pontikes Yiannis
Published: November 2019

Volumetric stability is an important aspect of the performance of building materials, and the shrinkage of CaO-FeOx-Al2O3-SiO2-rich inorganic polymers (IPs) has not been thoroughly investigated yet. Hence, this paper describes the outcome of a study conducted to investigate ways to minimize their shrinkage using different curing regimes. Two different slags were used as case studies to assess the robustness of the developed mitigation strategies. IP pastes and mortars were cured at (i) room condition, (ii) in slightly elevated temperature (60 °C for 2 d) and (iii) in a water-saturated environment. The reaction kinetics and formed products were examined on IP pastes, while mortars were made to characterize the 28 d pore structure, autogenous shrinkage, drying shrinkage, and strength development. The results showed that the precursors’ reactivity and curing conditions severely affect shrinkage mechanisms and magnitude. Volumetric changes in the plastic stage can be related to the precursors’ reactivity but drying shrinkage was the driving mechanism affecting the volumetric stability of all IP mortars. Understanding the effect of a precursor’s composition and curing conditions on shrinkage is fundamental to develop proper mitigation strategies and to overcome one of IPs’ main technical drawbacks.
Evaluation of the Thermal and Shrinkage Stresses in Restrained High-Performance Concrete
Authors: Yang Yang , Ma Linhao , Huang Jie , Gu Chunping , Xu Zhenjian , Liu Jintao , Ni Tongyuan
Published: November 2019

The early age volume deformation is the main course for the cracking of high-performance concrete (HPC). Hence, the shrinkage behavior and the restrained stress development of HPC under different restraints and curing conditions were experimentally studied in this paper. The method to separate the stress components in the total restraint stress was proposed. The total restrained stress was separated into autogenous shrinkage stress, drying shrinkage stress and thermal stress. The results showed that the developments of the free shrinkage (autogenous shrinkage and drying shrinkage) and the restrained stress were accelerated when the drying began; but the age when the drying began did not significantly influence the long-term shrinkage and restrained stress of HPC; the autogenous shrinkage stress continuously contributed to the development of the total restrained stress in HPC; the drying shrinkage stress developed very rapidly soon after the drying began; and the thermal stress was generated when the temperature dropped. The thermal stress was predominant at the early age, but the contributions of the three stresses to the total restrained stress were almost the same at the age of 56 d in this study.
Evaluating the Wear of Resin Teeth by Different Opposing Restorative Materials
Authors: Jin Seunglee , Choi Jae-Won , Jeong Chang-Mo , Huh Jung-Bo , Lee So-Hyoun , Lee Hyeonjong , Yun Mi-Jung
Published: November 2019

The aim of this study was to evaluate the wear properties of resin teeth with different opposing dental restorative materials. One type of resin tooth (Trubyte Biotone) was tested against six types of restorative materials including type III gold alloy (GO), monolithic zirconia (MZ), lithium disilicate glass ceramic (LD), nickel–chromium alloy (NC), feldspathic ceramic (FC), and steatite (ST). Two-body wear tests were performed under a vertical load of 5 kgf and thermo-cycling at 5/55 °C with a total of 120,000 cycles. The wear amount was quantified by measuring the volume loss of the resin teeth and the vertical substance loss of the opposing materials using three-dimensional images. The FC group showed a significantly greater amount of wear of the resin teeth, followed by the ST, NC, LD, MZ, and GO groups. The GO group showed significantly less wear of resin teeth than the other groups. There were no statistically significant differences in the wear of opposing restorative materials between groups. Within the limits of this study, it is recommended that zirconia, rather than feldspathic ceramic, should be used for restorations in the esthetic zone, and gold alloy should be used for areas with little or no esthetic demand.
Effect of TiB Orientation on Near-Threshold Fatigue Crack Propagation in TiB-Reinforced Ti-3Al-2.5V Matrix Composites Treated with Heat Extrusion
Authors: Kikuchi Shoichi , Tamai Shunsuke , Kawai Takao , Nakai Yoshikazu , Kurita Hiroki , Gourdet Sophie
Published: November 2019

TiB-reinforced Ti-3Al-2.5V matrix composites, in which TiB whiskers are oriented parallel to the direction of heat extrusion, were fabricated via mechanical alloying and hot isostatic pressing (HIP). To investigate the near-threshold fatigue crack propagation in TiB-reinforced Ti-3Al-2.5V matrix composites, stress intensity factor K-decreasing tests were conducted for disk-shaped compact specimens having two different orientations of TiB whiskers at force ratios from 0.1 to 0.8 under ambient conditions. The crack growth rates, da/dN, for the composites incorporating TiB whiskers oriented perpendicular to the direction of crack growth were constantly lower than those obtained in the case where the orientation was parallel at the same stress intensity range ΔK, while the threshold stress intensity range, ΔKth, was higher. This effect can be explained by the increase in the degree of roughness-induced crack closure resulting from the perpendicular TiB, because fatigue cracks preferentially propagated across the boundaries between the matrix and the TiB in certain regions. In contrast, the effective threshold stress intensity range, ΔKeff,th, for composites was unaffected by the TiB orientation at low force ratios.
Nanoindentation of Aluminum Single Crystals: Experimental Study on Influencing Factors
Authors: Filippov Pavel , Koch Ursula
Published: November 2019

Results from nanoindentation of aluminum single crystals deliver valuable information as model systems for understanding technical aluminum alloys. The effect of the crystal orientation and the azimuthal indenter orientation on indentation hardness and modulus was studied by Vickers indentation (max. load 10 mN) on single crystal surfaces with (100), (110), and (111) orientations. The average indentation hardness varied, depending on the crystallographic orientation, by 1.8%. The anisotropy of the elastic modulus (1.1% of the average modulus) is lowered (indentation averaging effect). This is predicted by explicit approximation of the contact problem (conical indenter, orthotropic material). It was found that indentation hardness and modulus vary periodically with the azimuthal indenter orientation on (100)- and (110)-oriented surfaces (relative amplitude of 1.8% for indentation hardness and 2.6% of the modulus). This is attributed to the combined effect of the indenter geometry and crystal symmetry. For the first time, this effect was quantified for aluminum single crystals.
Mechanical and Microstructural Characterization of Arc-Welded Inconel 625 Alloy
Authors: Kotzem Daniel , Beermann Lucas , Awd Mustafa , Walther Frank
Published: November 2019

The objective of this work was to verify a relatively new fusion-based additive manufacturing (AM) process to produce a high-temperature aerospace material. The nickel-based superalloy Inconel 625 (IN625) was manufactured by an arc-based AM technique. Regarding microstructure, typical columnar-oriented dendritic structure along the building direction was present, and epitaxial growth was visible. The mechanical behavior was characterized by a combination of quasi-static tensile and compression tests, whereas IN625 showed high yield and ultimate tensile strength with a maximum fracture strain of almost 68%. Even quasi-static compression tests at room and elevated temperatures (650 °C) showed that compression strength only slightly decreased with increasing temperature, demonstrating the good high-temperature properties of IN625 and opening new possibilities for the implementation of arc-based IN625 in future industrial applications.
Quantitative Structure-Activity Relationship Model to Predict Antioxidant Effects of the Peptide Fraction Extracted from a Co-Culture System of Chlorella pyrenoidosa and Yarrowia lipolytica
Authors: Liu Huifan , Li Sufen , Zhong Yuming , Liu Jianliang , Liu Hui , Cheng Jian , Ma Lukai , Huang Yuqing , Cai Xuanyi , Liu Haijun , Zheng Jiantong , Su Zhongai , Wang Qin
Published: November 2019

In this study, the antioxidant components in co-culture of Chlorella pyrenoidosa and Yarrowia lipolytica (3:1 ratio) were confirmed as trypsin-hydrolyzed peptides (EHPs). The EHPs were composed of 836 different peptides with molecular weights ranging from 639 to 3531 Da and were mainly composed of hydrophobic amino acids (48.1%). These peptides showed remarkable protective effects against oxidative stress in HepG2, which may be attributed to their structures. Furthermore, the mRNA and protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) were significantly lower in the peptide-treated group than in the control group, suggesting that the antioxidant enzyme-coding genes were not activated. The EC50 value of three peptides in the EHPs were in the order of AGYSPIGFVR (0.04 ± 0.002 mg/mL) > VLDELTLAR (0.09 ± 0.001 mg/mL) > LFDPVYLFDQG (0.41 ± 0.03 mg/mL); these results agreed with the prediction of the model (R2 > 0.9, Q2 > 0.5). Thus, EHPs show potential as potent new antioxidant agents.