bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced BSA Detection Precision: Leveraging High-Performance Dual-Gate Ion-Sensitive Field-Effect-Transistor Scheme and Surface-Treated Sensing Membranes
Authors: Kim Yeong-Ung , Cho Won-Ju
Published: March 2024

Bovine serum albumin (BSA) is commonly incorporated in vaccines to improve stability. However, owing to potential allergic reactions in humans, the World Health Organization (WHO) mandates strict adherence to a BSA limit (≤50 ng/vaccine). BSA detection with conventional techniques is time-consuming and requires specialized equipment. Efficient alternatives such as the ion-sensitive field-effect transistor (ISFET), despite rapid detection, affordability, and portability, do not detect BSA at low concentrations because of inherent sensitivity limitations. This study proposes a silicon-on-insulator (SOI) substrate-based dual-gate (DG) ISFET platform to overcome these limitations. The capacitive coupling DG structure significantly enhances sensitivity without requiring external circuits, owing to its inherent amplification effect. The extended-gate (EG) structure separates the transducer unit for electrical signal processing from the sensing unit for biological detection, preventing chemical damage to the transducer, accommodating a variety of biological analytes, and affording easy replaceability. Vapor-phase surface treatment with (3-Aminopropyl) triethoxysilane (APTES) and the incorporation of a SnO2 sensing membrane ensure high BSA detection efficiency and sensitivity (144.19 mV/log [BSA]). This DG-FET-based biosensor possesses a simple structure and detects BSA at low concentrations rapidly. Envisioned as an effective on-site diagnostic tool for various analytes including BSA, this platform addresses prior limitations in biosensing and shows promise for practical applications.
Neurite Orientation Dispersion and Density Imaging (NODDI) of Brain Microstructure in Adolescent Cannabis and Nicotine Use
Authors: Wallace Alexander L , Courtney Kelly E. , Wade Natasha E. , Hatz Laura E. , Baca Rachel , Jacobson Aaron , Liu Thomas T. , Jacobus Joanna
Published: March 2024

Introduction: Despite evidence suggesting deleterious effects of cannabis and nicotine tobacco product (NTP) use on white matter integrity, there have been limited studies examining white matter integrity among users of both cannabis and nicotine. Further, updated white matter methodology provides opportunities to investigate use patterns on neurite orientation dispersion and density (NODDI) indices and subtle tissue changes related to the intra- and extra-neurite compartment. We aimed to investigate how cannabis and NTP use among adolescents and young adults interacts to impact the white matter integrity microstructure. Materials and Methods: A total of 221 participants between the ages of 16 and 22 completed the Customary Drinking and Drug Use Record (CDDR) to measure substance use, and underwent a magnetic resonance imaging (MRI) session. Participants were divided into NTP-control and NTP groupings and cannabis-control and cannabis groupings (≥26 NTP/cannabis uses in past 6 months). Tract-Based Spatial Statistics (TBSS) and two-way between-subjects ANOVA investigated the effects of NTP use group, cannabis use group, and their interaction on fractional anisotropy (FA) and NODDI indices while controlling for age and biological sex. Results: NTP use was associated with decreased FA values and increased orientation dispersion in the left anterior capsule. There were no significant effects of cannabis use or the interaction of NTP and cannabis use on white matter outcomes. Discussion: NTP use was associated with altered white matter integrity in an adolescent and young adult sample. Findings suggest that NTP-associated alterations may be linked to altered fiber tract geometry and dispersed neurite structures versus myelination, as well as differential effects of NTP and cannabis use on white matter structure. Future work is needed to investigate how altered white matter is related to downstream behavioral effects from NTP use.
The Effect of Time Monitoring on the Development of Time-Based Prospective Memory among Children Aged 7–11 Years Old
Authors: Wang Yinya , Ren Zhi , Yue Yaqi , Zheng Xi , Zhang Xinyuan , Wang Lijuan
Published: March 2024

Time-based prospective memory (TBPM) refers to the ability of an individual to successfully execute an expected plan in the future at a certain time point or after a definite period of time. This study investigated the potential developmental mechanism of school-age children’s TBPM ability from the perspective of time monitoring. Experiment 1 used a between-subjects design of three ages (7, 9, 11) × two TBPM types (time point, time period) to investigate the trends and characteristics of two types of TBPM in children aged 7–11 years old. Experiment 2 used a between-subjects design of three ages (7, 9, 11) × two monitoring conditions (free monitoring, fixed monitoring) to investigate differences in two types of TBPM and monitoring behavior among school-age children under different monitoring conditions. These results showed that the age effect of TBPM was affected by the type of prospective memory (PM) and that time-point PM performance was significantly better than time-period PM performance among school-age children. These findings indicate that clear and definite external cues are helpful for school-age children in performing TBPM tasks. Moreover, there were significant differences found in the TBPM performance of school-age children under different time monitoring conditions. The performance of older children was significantly better than that of younger children. This indicates that older children can better allocate their attentional resources and use time monitoring strategies to improve their performance in PM tasks. Accordingly, this study showed that the TBPM ability of 7- to 11-year-old children is in continuous development and that the time monitoring behavior in the TBPM task is affected by task type and monitoring conditions.
Differences between Daylighting and Electric Lighting in Affective Response
Authors: Castilla Nuria , Blanca-Giménez Vicente , Pérez-Carramiñana Carlos , Llinares Carmen
Published: March 2024

Humans are spending more time indoors than ever due to urbanisation and industrialisation, leading to higher electricity consumption in lighting systems. Recent research has demonstrated the significance of maintaining a balance between daylight and electric light to create an ideal learning environment that can significantly impact students’ academic performance. The objective of this study is to analyse the changes in students’ emotional response depending on the type of lighting in the classroom—whether it is daylight, electric light, or a combination of both. A field study was conducted with 521 university students to assess their affective response to the lighting environment inside their classroom. The results show that students prefer a Clear-efficient lighting environment for writing–reading tasks and a Soft-calm atmosphere for using electronic devices. For the paying attention tasks, a combination of daylighting and electric lighting is determined to be the best solution, while for the tasks of discussing–teamwork, students prefer daylighting. Daylighting is found to be the only lighting option that students like. Despite this, students still consider electric lighting and the combination of daylight and electric light adequate for a classroom. The findings of this study may help educators and designers create learning spaces that promote a positive and stimulating student environment by understanding the relationship between the lighting environment and students’ affective responses.
An Analysis of South Korean Apartment Complex Types by Period Using Deep Learning
Authors: Yoon Sung-Bin , Hwang Sung-Eun , Kang Boo Seong , Lee Ji Hwan
Published: March 2024

The surge in demand for upscale apartments in South Korea in the 2000s necessitates the enhanced quality of apartment complexes. Achieving this improvement involves creating diverse spaces within complexes and categorizing them based on spatial arrangement. However, obtaining actual as-built drawings poses challenges, and manual analysis lacks objectivity. The study utilized map API for data collection and Roboflow API for labeling, employing a YOLOv8n-cls model for categorization. Performance evaluation included accuracy, precision, recall, and F1-score values using a confusion matrix. Eigen-CAM was utilized for an analysis that revealed the specific features influencing predictions. The classification model demonstrated relatively high accuracy. Furthermore, the prediction performance was high for lattice and square apartment complexes but low for distributed apartment complexes. These results indicate that a classification model is insufficient for assessing complex characteristics such as the scattered arrangement of building layouts and outdoor spaces, as seen in distributed apartment complexes. We determined that an in-depth analysis of the architectural plans for distributed apartment complexes is necessary to clearly identify their types, and the types must be categorized into several classes, including the distributed type.
A Novel Pervious Concrete Improved by Hexagonal Boron Nitride and Basalt Fiber in Mechanical Properties, Permeability, and Micro-Mechanisms
Authors: Zhan Qianqian , Yin Changjun
Published: March 2024

In order to overcome the limitations of traditional pervious concrete, which is difficult to balance in terms of both mechanical properties and permeability, this study proposed a novel and effective approach to improve the performances of pervious concrete (PC) based on hexagonal boron nitride (h-BN) and basalt fibers (BF). The mechanical properties and permeability tests of PC with single-doped or double-doped h-BN and BF were conducted first. Then the influence laws of h-BN and BF content on the compressive strength, flexural strength, porosity, and permeability coefficient for PC were revealed. Finally, the micro-mechanism effects of h-BN and BF on the performances of PC were explored by using a scanning electron microscope and an energy dispersive spectrometer. The results showed that the compressive strength of PC was increased with the increase in the h-BN content, and the flexural strength, porosity, and permeability coefficient increased first and then decreased. Meanwhile, with the increase in the BF content, the compressive strength and flexural strength of PC increased first and then decreased. Moreover, the compressive strength, flexural strength, porosity, and permeability coefficient of the proposed pervious concrete were 22.8 MPa, 5.17 MPa, 18.5%, and 5.09 mm/s, respectively, which were increases of 21.9%, 19.7%, 60.9%, and 42.2%, respectively, compared with ordinary permeable concrete when the optimal admixture combination was 15% fly ash, 0.08% h-BN, and 2.25% BF. This study can avoid the limitations of traditional pervious concrete and provide an efficient alternative way for improving the mechanical and permeability properties of pervious concrete.
Comprehensive Evaluation of Soil Improvement Benefits of Biological Retention Facilities Based on AHP-CRITIC
Authors: Liang Hui , Wu Meng , Jia Xingli , Yang Qi
Published: March 2024

Biological retention facilities are widely used in the construction of new urban infrastructure because of the increasingly serious problems of urban waterlogging and rainwater pollution. At present, a common method to resolve these problems is to improve the treatment performance of these facilities to improve the soil filling layer. How to evaluate the comprehensive benefits of the improved soil filling layer is a problem that cannot be ignored. In this paper, data from seven groups of soil improvement schemes were collected through laboratory tests, and an evaluation method based on AHP-CRITIC was proposed. Firstly, the evaluation indexes of soil improvement in biological retention facilities were determined, and their subjective and objective weights were determined by the AHP method and CRITIC method, respectively, and the comprehensive weights were further calculated. A comprehensive evaluation model was established by the TOPSISI method. Finally, the AHP-CRITIC evaluation model determined that 10% zeolite-amended soil had the best comprehensive evaluation effect, followed by 20% zeolite-amended soil and 10% gravel-amended soil. The results show that the evaluation model can comprehensively assess the benefits of soil improvement in bioretention facilities from the levels of infiltration, purification, and economy and can then select the optimal program for use in actual engineering practice.
Optimization of the Mechanical Properties of Bolted Connections between Concrete-Filled Tubular Columns and Steel Beam with Reinforcing Rings
Authors: Pan Zhicheng , Ma Fanjun , Cao Bing , Mo Zongyun , Liu Jing , Shi Ruoli , He Zhijian
Published: March 2024

To study the mechanical performance of bolted connections with different structural forms of reinforced rings, based on the results of monotonic loading tests on two bolted connections between a concrete-filled steel tubular column and a steel beam with an outer reinforcing ring, this article uses ABAQUS v.2020 software to establish a three-dimensional refined finite element analysis model of such connections using appropriate constitutive models for concrete and steel. Subsequently, the effect of the dimensions of the steel beam, reinforcing ring, and cover plate on the load-bearing properties and the failure mechanism of the connections is investigated, and the numerical model is consistent with the verification test results. Then, the numerical simulations comparing bolted exterior reinforced rings under seven different construction measures (i.e., number of bolts, stiffeners) based on a conventional welded exterior reinforced rings with rigid connections (i.e., CGJ) are standardized. The research results indicate that when four rows of bolts are introduced on exterior reinforced rings, the web of steel beam is welded with stiffeners, and the top and bottom reinforced rings are also added with stiffeners; this bolted connection with an external reinforcing ring (i.e., GZ-7) can achieve the rigidity and load-bearing capacity of a fully welded external reinforcing ring rigid connection. At the same time, the reinforcing ring plate is bolted to the flange of the steel beam, and the force transmission path at the connection is changed to avoid the brittle fracture easily caused by the welded flange joints. It is also in line with the development trend of sustainable construction of “assembly” and “disassembly”.
Contrasting Roles of Programmed Death-Ligand 1 Expression in Tumor and Stroma in Prognosis of Esophageal Squamous Cell Carcinoma
Authors: Murakami Tomohiro , Booka Eisuke , Furuhashi Satoru , Sakai Yuki , Sekimori Kenichi , Haneda Ryoma , Fujihiro Mayu , Matsumoto Tomohiro , Morita Yoshifumi , Kikuchi Hirotoshi , Hiramatsu Yoshihiro , Baba Satoshi , Takeuchi Hiroya
Published: March 2024

The assessment of programmed death-ligand 1 (PD-L1) expression in esophageal squamous cell carcinoma (ESCC) has become increasingly important with the rise of immune checkpoint inhibitors (ICIs). However, challenges persist, including subjective interpretation and the unclear significance of staining intensity, as well as contrasting roles in tumoral and stromal regions. Our study enhances the understanding of PD-L1 in ESCCs by analyzing its expression in tumors and stroma with H-scores, highlighting its distinct clinicopathological impacts. In a retrospective cohort of 194 ESCC specimens from surgical resection, we quantified PD-L1 expression in tumoral and stromal compartments using H-scores, analyzing whole slide images with digital pathology analysis software. Kaplan–Meier analysis demonstrated that higher PD-L1 expression is significantly associated with improved postoperative overall survival (OS) and recurrence-free survival (RFS) in both tumoral and stromal areas. Multivariable analysis identified high tumoral PD-L1 expression as an independent prognostic factor for prolonged OS and RFS (HR = 0.47, p = 0.007; HR = 0.54, p = 0.022, respectively). In a separate analysis, high stromal PD-L1 expression was found to correlate with less advanced pathological stages and a prolonged response to cytotoxic chemotherapy, with no similar correlation found for ICI treatment response. This study reveals PD-L1’s contrasting role in the ESCC tumor immune microenvironment, impacting prognosis, tumor stage, and treatment response.
Follicle-Stimulating Hormone Receptor Expression and Its Potential Application for Theranostics in Subtypes of Ovarian Tumors: A Systematic Review
Authors: Bakker Marie-Christine E. , Brink Geertruid J. , Poot Alex J. , Braat Arthur J. A. T. , Jonges Geertruida N. , Zweemer Ronald P.
Published: March 2024

Ovarian cancer mortality rates have not decreased significantly in the past years. As most women are still diagnosed in an advanced stage, there is a need for new treatment strategies for recurrent disease. A potentially new developing targeted approach, theranostics, combines diagnostics and treatment using radiopharmaceuticals. Through target receptors, imaging and treatment of malignant tissue can be achieved. For ovarian malignancy, the follicle-stimulating hormone (FSH) receptor may serve as a possible target since expression appears to be limited to ovarian cells. In this systematic review, we aim to gather all available literature on the expression of the FSH receptor in ovarian tumors. Pubmed, Embase and the Cochrane databases were searched until December 2023 for eligible studies. The search yielded 41 studies, mostly regarding serous carcinomas, sex cord–stromal tumors (SCSTs) and cell lines of serous and SCSTs. Various techniques were used to analyze the expression of the FSH receptor. For serous carcinomas, conflicting results on the expression of the FSH receptor were found. Studies on SCSTs, mainly studying the subtype of granulosa cell tumors, all showed positive expression of the FSH receptor. In the cell lines studies, the KGN cell line derived from a granulosa cell tumor shows positive expression in all studies. Available studies show that SCSTs express the FSH receptor. A theranostic approach targeting the FSH receptor may, therefore, provide a useful new approach for this malignancy with limited therapeutic options in recurrent disease.
Mechanical Stress Induces Sodium Entry and Osmoprotective Responses in Murine Synovial Fibroblasts
Authors: Proff Annemarie , Nazet Ute , Schröder Agnes , Jantsch Jonathan
Published: March 2024

Osteoarthritis (OA) is a multifactorial disease depending on molecular, genetic, and environmental factors like mechanical strain. Next to the cartilage and the subchondral bone, OA also affects the synovium, which is critically involved in the maintenance of joint homeostasis. As there is a correlation between the extracellular sodium content in the knee joint and OA, this study investigates the impact of sodium on OA-associated processes like inflammation and bone remodeling without and with mechanical loading in synovial fibroblasts. For that purpose, murine synovial fibroblasts from the knee joint were exposed to three different extracellular sodium chloride concentrations (−20 mM, ±0 mM and +50 mM NaCl) in the absence or presence of compressive or intermittent tensile strain. In addition to the intracellular Na+ content and gene expression of the osmoprotective transcription factor nuclear factor of activated T cells 5 (Nfat5), the gene and protein expression of inflammatory mediators (interleukin-6 (IL6), prostaglandin endoperoxide synthase-2 (Ptgs2)/prostaglandin E2 (PGE2)), and factors involved in bone metabolism (receptor activator of NF-κB ligand (RANKL), osteoprotegerin (OPG)) were analyzed by qPCR and ELISA. Mechanical strain already increased intracellular Na+ and Nfat5 gene expression at standard salt conditions to levels obtained by exposure to increased extracellular Na+ content. Both high salt and compressive strain resulted in elevated IL6 and PGE2 release. Intermittent tensile strain did not increase Il6 mRNA expression or IL6 protein secretion but triggered Ptgs2 expression and PGE2 production. Increased extracellular Na+ levels and compressive strain increased RANKL expression. In contrast, intermittent tension suppressed RANKL expression without this response being subject to modification by extracellular sodium availability. OPG expression was only induced by compressive strain. Changes in extracellular Na+ levels modified the inflammatory response and altered the expression of mediators involved in bone metabolism in cells exposed to mechanical strain. These findings indicate that Na+ balance and Nfat5 are important players in synovial fibroblast responses to mechanical stress. The integration of Na+ and Na+-dependent signaling will help to improve the understanding of the pathogenesis of osteoarthritis and could lead to the establishment of new therapeutic targets.
A Recombinant Peptide Device Combined with Adipose Tissue-Derived Stem Cells Enhances Subcutaneous Islet Engraftment
Authors: Mizui Takahiro , Inagaki Akiko , Nakamura Yasuhiro , Imura Takehiro , Uematsu Satomi Suzuki , Miyagi Shigehito , Kamei Takashi , Unno Michiaki , Watanabe Kimiko , Goto Masafumi
Published: March 2024

Subcutaneous space has been considered an attractive site for islet graft transplantation; however, the oxygen tension and vascularization are insufficient for islet graft survival. We investigated whether subcutaneous pre-implantation of a recombinant peptide (RCP) device with adipose tissue-derived stem cells (ADSCs) enhanced subcutaneous islet engraftment. RCP devices with/without syngeneic ADSCs were pre-implanted into the subcutaneous space of C57BL/6 mice. Syngeneic islets (300 or 120 islet equivalents (IEQs)) were transplanted into the pre-treated space after diabetes induction using streptozotocin. The cure rates of groups in which RCP devices were implanted four weeks before transplantation were significantly better than the intraportal transplantation group when 300 IEQs of islets were transplanted (p < 0.01). The blood glucose changes in the RCP+ADSCs-4w group was significantly ameliorated in comparison to the RCP-4w group when 120 IEQs of islets were transplanted (p < 0.01). Immunohistochemical analyses showed the collagen III expression in the islet capsule of the RCP+ADSCs-4w group was significantly enhanced in comparison to the RCP-4w and RCP+ADSCs-d10 groups (p < 0.01, p < 0.01). In addition, the number of von Willebrand factor-positive vessels within islets in the RCP+ADSCs-4w group was significantly higher than the RCP-4w group. These results suggest that using ADSCs in combination with an RCP device could enhance the restoration of the extracellular matrices, induce more efficient prevascularization within islets, and improve the graft function.
Molecular Mechanism of Autophagosome–Lysosome Fusion in Mammalian Cells
Authors: Ke Po-Yuan
Published: March 2024

In eukaryotes, targeting intracellular components for lysosomal degradation by autophagy represents a catabolic process that evolutionarily regulates cellular homeostasis. The successful completion of autophagy initiates the engulfment of cytoplasmic materials within double-membrane autophagosomes and subsequent delivery to autolysosomes for degradation by acidic proteases. The formation of autolysosomes relies on the precise fusion of autophagosomes with lysosomes. In recent decades, numerous studies have provided insights into the molecular regulation of autophagosome–lysosome fusion. In this review, an overview of the molecules that function in the fusion of autophagosomes with lysosomes is provided. Moreover, the molecular mechanism underlying how these functional molecules regulate autophagosome–lysosome fusion is summarized.
Leucine Supplementation Prevents the Development of Skeletal Muscle Dysfunction in a Rat Model of HFpEF
Authors: Alves Paula Ketilly Nascimento , Schauer Antje , Augstein Antje , Prieto Jarabo Maria-Elisa , Männel Anita , Barthel Peggy , Vahle Beatrice , Moriscot Anselmo S. , Linke Axel , Adams Volker
Published: March 2024

Heart failure with preserved ejection fraction (HFpEF) is associated with exercise intolerance due to alterations in the skeletal muscle (SKM). Leucine supplementation is known to alter the anabolic/catabolic balance and to improve mitochondrial function. Thus, we investigated the effect of leucine supplementation in both a primary and a secondary prevention approach on SKM function and factors modulating muscle function in an established HFpEF rat model. Female ZSF1 obese rats were randomized to an untreated, a primary prevention, and a secondary prevention group. For primary prevention, leucine supplementation was started before the onset of HFpEF (8 weeks of age) and for secondary prevention, leucine supplementation was started after the onset of HFpEF (20 weeks of age). SKM function was assessed at an age of 32 weeks, and SKM tissue was collected for the assessment of mitochondrial function and histological and molecular analyses. Leucine supplementation prevented the development of SKM dysfunction whereas it could not reverse it. In the primary prevention group, mitochondrial function improved and higher expressions of mitofilin, Mfn-2, Fis1, and miCK were evident in SKM. The expression of UCP3 was reduced whereas the mitochondrial content and markers for catabolism (MuRF1, MAFBx), muscle cross-sectional area, and SKM mass did not change. Our data show that leucine supplementation prevented the development of skeletal muscle dysfunction in a rat model of HFpEF, which may be mediated by improving mitochondrial function through modulating energy transfer.
Inflammatory Skin Diseases: Focus on the Role of Suppressors of Cytokine Signaling (SOCS) Proteins
Authors: Cianciulli Antonia , Calvello Rosa , Porro Chiara , Lofrumento Dario Domenico , Panaro Maria Antonietta
Published: March 2024

Inflammatory skin diseases include a series of disorders characterized by a strong activation of the innate and adaptive immune system in which proinflammatory cytokines play a fundamental role in supporting inflammation. Skin inflammation is a complex process influenced by various factors, including genetic and environmental factors, characterized by the dysfunction of both immune and non-immune cells. Psoriasis (PS) and atopic dermatitis (AD) are the most common chronic inflammatory conditions of the skin whose pathogeneses are very complex and multifactorial. Both diseases are characterized by an immunological dysfunction involving a predominance of Th1 and Th17 cells in PS and of Th2 cells in AD. Suppressor of cytokine signaling (SOCS) proteins are intracellular proteins that control inflammatory responses by regulating various signaling pathways activated by proinflammatory cytokines. SOCS signaling is involved in the regulation and progression of inflammatory responses in skin-resident and non-resident immune cells, and recent data suggest that these negative modulators are dysregulated in inflammatory skin diseases such as PS and AD. This review focuses on the current understanding about the role of SOCS proteins in modulating the activity of inflammatory mediators implicated in the pathogenesis of inflammatory skin diseases such as PS and AD.
The Effect of Leukocyte Removal and Matrix Metalloproteinase Inhibition on Platelet Storage Lesions
Authors: Rak-Pasikowska Alina , Hałucha Kornela , Sapa-Wojciechowska Agnieszka , Wrzyszcz Aneta , Gałuszka Wioletta , Pęcak-Solińska Anna , Bil-Lula Iwona
Published: March 2024

The reasons for unfavorable changes in platelet concentrate (PC) quality during storage are not fully understood yet. We aimed to evaluate whether leukocytes and matrix metalloproteinases (MMPs) lead to a decrease in the quality of PCs and examine whether MMP inhibition will slow down the platelets’ aging. Nine PCs were divided into three parts: (1) leukocyte-depleted (F) PCs, (2) PCs with no additional procedures (NF), and (3) PCs with the addition of an MMP inhibitor—doxycycline (D). Each PC was stored for 144 h, and a sample for testing was separated from each part on the day of preparation and after 24, 48, 72 and 144 h of storage. Blood morphological analysis, platelet aggregation, and the expression of activation markers were evaluated. MMP-2 and MMP-9 concentration, activity, and gene expression were assessed. Platelet aggregation decreased, and platelet activation marker expression increased during the storage. D concentrates showed the lowest level of platelet activation. In turn, leukocyte-depleted PCs showed the highest level of platelet activation in general. MMP-9 platelet activity was higher in leukocyte-containing concentrates at the end of the storage period. We concluded that the filtration process leads to a higher platelet activation level. The presence of doxycycline in PCs reduces the expression of the activation markers as compared to leukocyte-depleted concentrates.
Hydrogen Permeation Properties of Ternary Ni–BaCe0.9Y0.1O3–Ce0.9Gd0.1O2 Cermet Membranes
Authors: Itagaki Yoshiteru , Mori Hiroyuki , Matsubayashi Takumi , Aono Hiromichi
Published: March 2024

A ternary Ni–BaCe0.9Y0.1O3 (BCY)–Ce0.9Gd0.1O2 (GDC) cermet involving 40 vol% Ni was fabricated, and its hydrogen permeation characteristics were evaluated when the GDC volume fraction was varied from 0 to 30 vol%. The X-ray diffraction results of the cermet after sintering at 1400 °C revealed that GDC was dissolved in BCY when the GDC volume composition was 20 vol%. Regardless of the BCY and GDC volume fractions, the metal conductivity of the cermet was dominated by Ni. After the addition of only 1 vol% GDC, the particle sizes of Ni and BCY in the cermet significantly decreased, and the particle size decreased as the volume fraction of GDC increased. The hydrogen permeability increased with increasing temperature and for up to 10 vol% GDC, and a maximum permeation rate of 0.142 mL min−1 cm−2 was obtained at 700 °C. This value is comparable to or better than previously reported values for Ni-cermets under the same conditions. The amount of hydrogen permeation decreased above 10 vol% GDC. This study demonstrated that Ni-BCY-GDC cermet is a material that has both high hydrogen permeability and CO2 resistance.
Gender Differences in the Co-Use of Tranquilizers, Sedatives, Sleeping Pills and Alcohol among Spanish Adolescents: A Nationwide Population-Based Study
Authors: Carrasco-Garrido Pilar , Jiménez-Trujillo Isabel , Hernández-Barrera Valentín , Florencio Lidiane Lima , Yeamans Spencer , Palacios-Ceña Domingo
Published: March 2024

Adolescence is a critical developmental stage for the initiation of substance use worldwide, which is one of the main risk-taking behaviors that may impact adolescents’ physical and mental well-being. The aims of this study were to (1) assess the prevalence of the co-use of tranquilizers, sedatives, and sleeping pills with alcohol (TSSp&AC) by gender in the Spanish adolescent population in 2018 and (2) identify the variables associated with TSSp&AC. An observational cross-sectional study following STROBE guidelines was conducted. We analyzed data from 38,010 adolescents aged 14 to 18 years old (18,579 males and 19,431 females) who participated in ESTUDES (Survey on Drug Use in Secondary Education in Spain) 2018. Female adolescents reported a higher prevalence of TSSp&AC than males (p < 0.001). The factors associated with female co-use were being 16–18 years of age (OR 1.65); the consumption of tobacco (OR 1.73), cocaine (OR 1.84), other illicit psychoactive drugs (OR 1.89); and novel illicit psychoactive drugs (OR 1.74); no perceived health risk from the consumption of TSSps (OR 2.45); and the perceived availability of TSSps (OR 2.23) and alcohol (OR 2.09). There are several factors associated with TSSp&AC in Spanish female adolescents with potential implications for healthcare providers.
Suspected Simple Appendicitis in Children: Should We Use a Nonoperative, Antibiotic-Free Approach? An Observational Study
Authors: Reis Wolfertstetter Patricia , Ebert John Blanford , Barop Judith , Denzinger Markus , Kertai Michael , Schlitt Hans J. , Knorr Christian
Published: March 2024

Background: Simple appendicitis may be self-limiting or require antibiotic treatment or appendectomy. The aim of this study was to assess the feasibility and safety of a nonoperative, antibiotic-free approach for suspected simple appendicitis in children. Methods: This single-center, retrospective study included patients (0–17 years old) who were hospitalized at the pediatric surgery department due to suspected appendicitis between 2011 and 2012. Data from patients who primarily underwent appendectomy were used as controls. The follow-up of nonoperatively managed patients was conducted in 2014. The main outcome of interest was appendicitis recurrence. Results: A total of 365 patients were included: 226 were treated conservatively and 139 underwent appendectomy. Fourteen (6.2% of 226) of the primarily nonoperatively treated patients required secondary appendectomy during follow-up, and histology confirmed simple, uncomplicated appendicitis in 10 (4.4% of 226) patients. Among a subset of 53 patients managed nonoperatively with available Alvarado and/or Pediatric Appendicitis Scores and sonographic appendix diameters in clinical reports, 29 met the criteria for a high probability of appendicitis. Three of these patients (10.3% of 29) underwent secondary appendectomy. No complications were reported during follow-up. Conclusions: A conservative, antibiotic-free approach may be considered for pediatric patients with suspected uncomplicated appendicitis in a hospital setting. Only between 6 and 10% of these patients required secondary appendectomy. Nevertheless, the cohort of patients treated nonoperatively was likely to have also included individuals with further abdominal conditions other than appendicitis. Active observation and clinical support during the disease course may help patients avoid unnecessary procedures and contribute to spontaneous resolution of appendicitis or other pediatric conditions as the cause of abdominal pain. However, further studies are needed to define validated diagnostic and management criteria.
Acute Effects of Walking and Standing on Executive Function in Children with Attention Deficit/Hyperactivity Disorder: A Feasibility Study
Authors: Barudin-Carreiro Amanda , Camhi Sarah M. , Stanish Heidi I. , Wright Julie A.
Published: March 2024

Children with attention deficit/hyperactivity disorder (ADHD) struggle with executive functioning (EF). While physical activity (PA) benefits EF, little is known about the impact of specific activities like standing. The purpose of this study was to evaluate the feasibility of performing a rigorous experimental study to compare the effects of walking and standing on EF in children with ADHD. Six areas of feasibility were assessed: recruitment, randomization, treatment adherence, retention, acceptability of the intervention, and implementation. A randomized pilot study using three parallel conditions compared the effects of two modes of activity on EF in children 6–11 with ADHD. While there were no significant differences between walking and standing for EF, analyses suggest that it is feasible to compare effects of standing vs. walking on EF among children with ADHD. This study supports the feasibility of undertaking a larger scale study to evaluate the effect of standing on EF in children with ADHD.
Clinician- and Patient-Centred Outcomes of Digital Impressions in Infants with Cleft Lip and Palate: A Systematic Review
Authors: Unnikrishnan Jyotsna , Etemad Shahidi Yasaman , Bakr Mahmoud , Love Robert , Idris Ghassan
Published: March 2024

This systematic review examines the effectiveness of digital impressions in infants with cleft lip and palate (CLP), focusing on accuracy, operator preferences, and parents’ perceptions. The PICO-formulated focused questions assessed the accuracy and operator preference of digital impressions compared to conventional impressions in infants with cleft lip and palate, while also exploring parents’ perceptions as patient-centred outcomes. Electronic and manual searches were conducted in five databases including PubMed, Scopus, Web of Science, Embase, and Cochrane Library; to acquire grey literature, Google Scholar was also consulted. Both experimental and observational studies that used digital impressions in the clinical care of infants with CLP were included. The Joanna Briggs Institute Critical Appraisal Checklist was used to assess the quality of the included studies. Out of 503 records, 12 studies met the inclusion criteria. The accuracy assessment included surface discrepancy and intra-arch measurements. Surface discrepancy studies showed variations in the premaxillary segments, while intra-arch measurements revealed no significant differences. Operators preferred digital impressions, citing reduced stress and streamlined workflows. Parents expressed a clear preference for digital over conventional impressions. The conclusions drawn were substantiated by weak evidence due to the limited number and the high risk of bias of the included studies. Challenges remain here, warranting continued research to enhance accuracy and assess parents’ preferences, ensuring optimal outcomes for infants with CLP.
Dimethyloxalylglycine Suppresses SREBP1c and Lipogenic Gene Expressions in Hepatocytes Independently of HIF1A
Authors: Kwon Yong Seong , Cho Ye Eun , Kim Yeonsoo , Koh Minseob , Hwang Seonghwan
Published: March 2024

Dimethyloxalylglycine (DMOG) is a representative inhibitor of the prolyl hydroxylase domain (PHD), which mediates the degradation of hypoxia-inducible factor-1-alpha (HIF1A). DMOG exerts its pharmacological effects via the canonical pathway that involves PHD inhibition; however, it remains unclear whether DMOG affects lipogenic gene expression in hepatocytes. We aimed to elucidate the effects of DMOG on sterol regulatory element-binding protein-1c (SREBP1c), a master regulator of fatty acid synthesis in hepatocytes. DMOG treatment inhibited SREBP1c mRNA and protein expression in HepG2 and AML12 hepatocytes and reduced the transcript levels of SREBP1c-regulated lipogenic genes. A luciferase reporter assay revealed that DMOG inhibited the transcriptional activity of SREBP1c. Moreover, DMOG suppressed SREBP1c expression in mice liver. Mechanistically, treatment with DMOG enhanced the expression of HIF1A and insulin-induced gene 2 (INSIG2), which inhibits the activation of SREBP1c. However, HIF1A or INSIG2 knockdown failed to reverse the inhibitory effect of DMOG on SREBP1c expression, suggesting a redundant role of HIF1A and INSIG2 in terms of repressing SREBP1c. DMOG did not function through the canonical pathway involving inhibition of SREBP1c by PHD, highlighting the presence of non-canonical pathways that mediate its anti-lipogenic effect.
Space–Time Characterization of Extreme Precipitation Indices for the Semiarid Region of Brazil
Authors: dos Santos Ana Letícia Melo , Gonçalves Weber Andrade , Andrade Lara de Melo Barbosa , Rodrigues Daniele Tôrres , Batista Flávia Ferreira , Lima Gizelly Cardoso , e Silva Cláudio Moisés Santos
Published: March 2024

Various indices of climate variability and extremes are extensively employed to characterize potential effects of climate change. Particularly, the semiarid region of Brazil is influenced by adverse effects of these changes, especially in terms of precipitation. In this context, the main objective of the present study was to characterize the regional trends of extreme precipitation indices in the semiarid region of Brazil (SAB), using daily precipitation data from the IMERG V06 product, spanning the period from 1 January 2001 to 31 December 2020. Twelve extreme precipitation indices were considered, which were estimated annually, and their spatial and temporal trends were subsequently analyzed using the nonparametric Mann–Kendall test and Sen’s slope. The analysis revealed that the peripheral areas of the SAB, especially in the northwest and extreme south regions, exhibited higher intensity and frequency of extreme precipitation events compared to the central portion of the area. However, a negative trend in event intensity was noted in the north, while positive trends were identified in the south. The frequency of extreme events showed a predominance of negative trends across most of the region, with an increase in consecutive dry days particularly throughout the western SAB. The average total precipitation index was above 1000 mm in the north of the SAB, whereas in the central region, the precipitation averages were predominantly below 600 mm, with rainfall intensity values ranging between 6 and 10 mm/day. Over the span of 20 years, the region underwent an average of 40 consecutive dry days in certain localities. A negative trend was observed in most of the indices, indicating a reduction in precipitation intensity in future decades, with variations in some indices. The dry years observed towards the end of the analyzed period likely contributed to the observed negative trends in the majority of extreme precipitation indices. Such trends directly impact the intensity and frequency of extreme weather events in the SAB. The study is important for highlighting and considering the impacts of changes in precipitation extremes in the semiarid region of Brazil. Based on the obtained results, we advocate the implementation of public policies to address future challenges, such as incorporating adaptations in water resource management, sustainable agricultural practices, and planning for urban and rural areas.
Understanding the Fracture Failure Mechanism of WC Particle-Reinforced FeCoCrNiMn High-Entropy Alloy Coatings at 600 °C
Authors: Wang Xinbo , Zhang Shihan , Zhao Fei , Wu Zhisheng , Xie Zhiwen
Published: March 2024

A detailed electron microscopy study was performed to clarify the fracture mechanism of WC particles reinforced with FeCoCrNiMn high-entropy alloy coatings at 600 °C. Large-sized fibers and elemental segregation formed in the coating, triggering high local stress in the matrix alloy and resulting in a low tensile strength of 150 MPa. High temperature promoted the homogenization process of elemental segregation, but also facilitated the dissolution of large-sized fibers, resulting in the growth of slim fibers and nanofibers. Both the structural homogenization and multi-scale fiber strengthening led to an enhanced tensile strength of 242 MPa at 600 °C. These current findings provide an understanding of the fracture mechanism of HEA/WC coatings during high-temperature exposure.
Temperature Analysis in Cubic Boron Nitrate Cutting Tool during Minimum Quantity Lubrication Turning with a Coconut-Oil-Based Nano-Cutting Fluid Using Computational Fluid Dynamics
Authors: Khetre Subhash , Bongale Arunkumar , Kumar Satish , Ramesh B. T.
Published: March 2024

The minimum quantity lubrication (MQL) approach is used for improving tool life at a low cost, and it is environmentally friendly. When compared to traditional flood cooling technology, the flow rate in MQL is thought to be 10,000 times lower. The workpiece’s surface smoothness is enhanced by continuous chip formation during turning, but because the tool is always in touch with the chip, a crater wear zone is formed on the rake face due to high friction and thermal stress. While adding nanoparticles to MQL enhances cutting performance, a high concentration of these nanoparticles causes burr adhesion and decreased chip evacuation capability due to the agglomeration of nanoparticles, which affects the surface finish of the workpiece. A novel “coconut-oil-based SiC–MWCNT nano-cutting fluid for a CBN insert cutting tool” is proposed in this approach to overcome these issues. Silicon carbide (SiC) and multi-walled carbon nanotubes (MWCNTs) are added to coconut oil with an appropriate volume fraction for better lubrication. The thermal properties of the proposed nano-cutting fluid are compared with those of some existing nano MQL cutting fluids, and it was found that the MQL cutting fluid under consideration exhibits an elevated thermal conductivity and convective heat transfer coefficient that efficiently reduce tool temperature and improve tool life. The comparative study between the Finite Element Simulation using computational fluid dynamics (CFD) predicted variation in tool temperature and the corresponding experimental values revealed a remarkable alignment with a marginal error ranging from 1.27% to 3.44%.
Mechanical Characterization of Resistance-Welded and Seamless API 5L X52 Pipes: A Comparative Study
Authors: Terán Méndez Gerardo , Capula-Colindres Selene Irais , Velázquez Julio César , Angeles-Herrera Daniel , González-Arévalo Noé Eliseo , Torres-Santillan Esther , Cervantes-Tobón Arturo
Published: March 2024

It is well known that the mechanical properties of a steel plate depend on the anisotropy of the material and the rolling directions. This paper presents the results of the Charpy V-Notch (CVN) impact test for the ST, TL, TS, LS, LT, 45°, and SL directions in API 5L X52 pipelines with electric-resistance-welded (ERW) and seamless (SMLS) pipes. Charpy specimens were manufactured and tested according to the ASTM E23 standard in laboratory conditions. All possible directions in the pipe were tested. Three Charpy specimens were tested for each direction, for a total of 21 Charpy tests. Moreover, the microstructures, hardness, ductile and brittle areas, and fracture surfaces of the Charpy specimens are presented in this research. The results show that the Charpy energy values, hardness, and microstructures depend on the direction of the specimens. The Charpy values of the SMLS pipe are higher than those of the ERW pipe because of several metallurgical factors, such as grain size, non-metallic inclusions, delaminations, and microstructures.
Obtaining Accurate Gold Prices
Authors: Sinha Amit K.
Published: March 2024

Gold prices have been of major interest for a lot of investors, analysts, and economists. Accordingly, a number of different modeling approaches have been used to forecast gold prices. In this manuscript, the geometric Brownian motion approach, used in the pricing of numerous types of assets, is used to forecast the prices of gold at yearly, monthly, and quarterly frequencies. This approach allows for simulating one-period-ahead prices and the associated probabilities. The expected prices obtained from the simulated prices and probabilities are found to provide reliable forecasts when compared with the observed yearly, monthly, and quarterly prices.
Accurate Height Determination in Uneven Terrains with Integration of Global Navigation Satellite System Technology and Geometric Levelling: A Case Study in Lebanon
Authors: Mustafin Murat , Moussa Hiba
Published: March 2024

The technology for determining a point’s coordinates on the earth’s surface using the global navigation satellite system (GNSS) is becoming the norm along with ground-based methods. In this case, determining coordinates does not cause any particular difficulties. However, to identify normal heights using this technology with a given accuracy, special research is required. The fact is that satellite determinations of geodetic heights (h) over an ellipsoid surface differ from ground-based measurements of normal height (HN) over a quasi-geoid surface by a certain value called quasi-geoid height or height anomaly (ζ). In relation to determining heights of a certain territory, the concept of geoid height (N) is usually operated when dealing with a geoid model. In this work, geodetic and normal heights are determined for five control points in three different regions in Lebanon, where measurements are carried out using GNSS technology and geometric levelling. The obtained quasi-geoid heights are compared with geoid heights derived from the global Earth model EGM2008. The results obtained showed that, in the absence of gravimetric data, the combination of global Earth model data, geometric levelling for selected areas, and satellite determinations allows for the creation of a highly accurate altitude network for mountainous areas.