bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Aloe Vera on healing of colonic anastomoses: experimental rat study
Authors: Tumay L. Volkan , Kılıcturgay Sadık , Savci Vahide , Saraydaroglu Ozlem , Anarat Ruksan
Published: November 2018

Background Although herbal medicinal products are being used widely throughout the World, beneficial and harmful effects have not been well documented. Our aim was to evaluate the effects of Aloe Vera (AV) on colonic anastomosis healing.

Material and methods 112 albino Wistar rats were randomly assigned into five main groups: preoperative Aloe Vera Group (P), pre- and postoperative Aloe Vera Group (PP), Control Group (C), sham Aloe Vera Group (SA) and Sham Control Group (SC). Groups P, PP, and SA received 1.6 mL/kg per day Aloe Vera by orogastric feeding catheter for 1 month prior to the experiment. Groups P, PP, and C underwent anastomosis of the distal colon, and subgroups (n = 4) of each were sacrificed on postoperative day 3, 7, 14 and 21. Anastomotic bursting pressure, perianastomotic collagen content and histopathological changes were studied.

Results The SC Group had significantly higher ABP when compared with the SA Group (p = 0.0002), although hydroxyproline content showed no difference. When ABP was compared between anastomosis groups, it was found significantly lower in Aloe Vera groups on Day 3 (P3 vs. C3, p = 0.003 and PP3 vs. C3, p = 0.007). Hydroxyproline content was significantly lower in Group PP than Group C, also on Day 3 (p = 0.05). Significant difference was not detected after Day 3 in any of the study parameters.

Conclusion  Aloe Vera decreased tissue collagen content in the early postoperative period. It is advisable to call into question the concomitant usage of conventional medicine and the herbal supplements for the surgeons in their clinical practice.
Evaluation of environmental cleaning of patient rooms: Impact of different fluorescent gel markers
Authors: Rock Clare , Xie Anping , Andonian Jennifer , Hsu Yea-Jen , Osei Patience , Keller Sara C. , Gurses Ayse P. , Trexler Polly , Maragakis Lisa L , Cosgrove Sara E. ,   for the CDC Prevention Epicenters Program
Published: November 2018

In this systematic evaluation of fluorescent gel markers (FGM) applied to high-touch surfaces with a metered applicator (MA) made for the purpose versus a generic cotton swab (CS), removal rates were 60.5% (476 of 787) for the MA and 64.3% (506 of 787) for the CS. MA-FGM removal interpretation was more consistent, 83% versus 50% not removed, possibly due to less varied application and more adhesive gel.
Developing a conceptual model for insider threat
Authors: Whitty Monica T
Published: November 2018

This paper sets out 99 case studies of insider attacks that took place in the UK. The study involved interviewing investigators, heads of security, information technologists, law enforcement, security officers, human resource managers, line managers, and coworkers who knew the insider. The analysis elucidates how to identify insiders and pathways to these attacks. It also highlights examples of archetypal insiders, in addition to the ‘disgruntled employee’ (e.g., ‘the show off’, ‘the career criminal’, ‘the addict’, etc.). In contrast to other studies, this study highlights multiple pathways to an attack. A conceptual model is set out that considers indicators (both physical and cyber) that might be monitored in an insider risk detection programme. The model stressors need to continuously seek out methods to close down opportunities as well as to monitor behavioural change. It also elucidates potential deterrence and prevention strategies for organisations to consider in an ethical and legal manner.
The association between dietary protein intake, energy intake and physical frailty: results from the Rotterdam Study
Authors: Schoufour Josje D. , Franco Oscar H. , Kiefte-de Jong Jessica C. , Trajanoska Katerina , Stricker Bruno , Brusselle Guy , Rivadeneira Fernando , Lahousse Lies , Voortman Trudy
Published: November 2018

Sufficient protein intake has been suggested to be important for preventing physical frailty, but studies show conflicting results which may be explained because not all studies address protein source and intake of other macronutrients and total energy. Therefore, we studied 2504 subjects with data on diet and physical frailty, participating in a large population-based prospective cohort among subjects aged 45+ years (the Rotterdam Study). Dietary intake was assessed with a FFQ. Frailty was defined according to the frailty phenotype as the presence of at least three out of the following five symptoms: weight loss, low physical activity, weakness, slowness and fatigue. We used multinomial logistic regression models to evaluate the independent association between protein intake and frailty using two methods: nutrient residual models and energy decomposition models. With every increase in 10 g total, plant or animal protein per d, the odds to be frail were 1·06 (95 % CI 0·98, 1·15), 0·87 (95 % CI 0·71, 1·07) and 1·07 (95 % CI 0·99, 1·15), respectively, using the nutrient residual method. Using the energy partition model, we observed that the odds to be frail were lower with higher vegetable protein intake (OR per 418·4 kJ (100 kcal): 0·61, 95 % CI 0·39, 0·97), however, results disappeared when adjusting for physical activity. For energy intake from any source we observed that with every 418·4 kJ (100 kcal) increase, the odds to be frail were 5 % lower (OR: 0·95, 95 % CI 0·93, 0·97). Our results suggest that energy intake, but not protein specifically, is associated with less frailty. Considering other macronutrients, physical activity and diet quality seems to be essential for future studies on protein and frailty.
Optimizing patient expectancy in the pharmacologic treatment of major depressive disorder
Authors: Zilcha-Mano Sigal , Brown Patrick J. , Roose Steven P. , Cappetta Kiley , Rutherford Bret R.
Published: November 2018

Background

Patient expectancy is an important source of placebo effects in antidepressant clinical trials, but all prior studies measured expectancy prior to the initiation of medication treatment. Little is known about how expectancy changes during the course of treatment and how such changes influence clinical outcome. Consequently, we undertook the first analysis to date of in-treatment expectancy during antidepressant treatment to identify its clinical and demographic correlates, typical trajectories, and associations with treatment outcome.

Methods

Data were combined from two randomized controlled trials of antidepressant medication for major depressive disorder in which baseline and in-treatment expectancy assessments were available. Machine learning methods were used to identify pre-treatment clinical and demographic predictors of expectancy. Multilevel models were implemented to test the effects of expectancy on subsequent treatment outcome, disentangling within- and between-patient effects.

Results

Random forest analyses demonstrated that whereas more severe depressive symptoms predicted lower pre-treatment expectancy, in-treatment expectancy was unrelated to symptom severity. At each measurement point, increased in-treatment patient expectancy significantly predicted decreased depressive symptoms at the following measurement (B = −0.45, t = −3.04, p = 0.003). The greater the gap between expected treatment outcomes and actual depressive severity, the greater the subsequent symptom reductions were (B = 0.49, t = 2.33, p = 0.02).

Conclusions

Greater in-treatment patient expectancy is associated with greater subsequent depressive symptom reduction. These findings suggest that clinicians may benefit from monitoring and optimizing patient expectancy during antidepressant treatment. Expectancy may represent another treatment parameter, similar to medication compliance and side effects, to be regularly monitored during antidepressant clinical management.
The influence of family and professional lifecourse histories on economic activity among older French workers
Authors: Ogg Jim , Renaut Sylvie
Published: November 2018

This paper examines associations between early and mid-lifecourse events with economic activity in later life. These lifecourse trajectories are in turn examined for their impact on the pathways of men and women to retirement, including whether these pathways are perceived by individuals as been chosen or imposed. Data are from the three waves (2005, 2008 and 2011) of the French version of the Gender and Generations survey and comprise a sub-sample of 2,016 respondents in the birth cohort 1941–1960 who participated in all three waves. The analysis is undertaken within a gender perspective and in the context of the (de)standardisation of the lifecourse. The results show that mid-life and later-life work history, job category, employment sector and economic activity are influenced by early lifecourse events for both men and for women. Different pathways to retirement are observed according to institutional factors that determine access to pension rights. Women whose family formation occurred early, together with women who had an absence of family events (partnership or childlessness), were much more likely to be economically active in later life than men with the same characteristics. The results suggest that institutionalised (standardised) lifecourse patterns exist simultaneously with individualised (destandardised) patterns.
The acquisition of word order in L2 Norwegian: The case of subject and object shift
Authors: Anderssen Merete , Bentzen Kristine , Busterud Guro , Dahl Anne , Lundquist Björn , Westergaard Marit
Published: November 2018

This article reports on a syntactic acceptability judgement study of 59 adult L2/Ln learners of Norwegian and a group of native controls, studying subject and object shift. These constructions involve movement of (mainly) pronominal subjects or objects across negation/adverbs. Both subject shift and object shift display considerable micro-variation in terms of syntax and information structure, dependent on factors such as nominal type (pronoun vs. full DP), function (subject vs. object), and information status (given vs. new/focused). Previous studies have shown that Norwegian children have an early preference for the unshifted position in both constructions, but that they acquire subject shift relatively early (before age 3). Object shift, on the other hand, is typically not in place until after age 6–7. Importantly, children are conservative learners, and never shift elements that should not move in the adult language. The results of the current study show that L2/Ln learners do not make all the fine distinctions that children make, in that they have a clear preference for all subjects in shifted position and all objects in unshifted position, although some distinctions fall into place with increased proficiency. Importantly, unlike children, the L2/Ln learners are not conservative learners; rather, they over-accept syntactic movement in several cases. The equivalent to this in language production would be to apply syntactic movement where it is not attested in the target language, which would be the opposite behaviour to that observed in L1 children.
Association between rs12252 and influenza susceptibility and severity: an updated meta-analysis
Authors: Chen T. , Xiao M. , Yang J. , Chen Y. K. , Bai T. , Tang X. J. , Shu Y. L.
Published: November 2018

In several lately published studies, the association between single-nucleotide polymorphism (SNP, rs12252) of IFITM3 and the risk of influenza is inconsistent. To further understand the association between the SNP of IFITM3 and the risk of influenza, we searched related studies in five databases including PubMed published earlier than 9 November 2017. Ten sets of data from nine studies were included and data were analysed by Revman 5.0 and Stata 12.0 in our updated meta-analysis, which represented 1365 patients and 5425 no-influenza controls from four different ethnicities. Here strong association between rs12252 and influenza was found in all four genetic models. The significant differences in the allelic model (C vs. T: odds ratio (OR) = 1.35, 95% confidence interval (CI) (1.03–1.79), P = 0.03) and homozygote model (CC vs. TT: OR = 10.63, 95% CI (3.39–33.33), P < 0.00001) in the Caucasian subgroup were discovered, which is very novel and striking. Also novel discoveries were found in the allelic model (C vs. T: OR = 1.37, 95% CI (1.08–1.73), P = 0.009), dominant model (CC + CT vs. TT: OR = 1.48, 95% CI (1.08–2.02), P = 0.01) and homozygote model (CC vs. TT: OR = 2.84, 95% CI (1.36–5.92), P = 0.005) when we compared patients with mild influenza with healthy individuals. Our meta-analysis suggests that single-nucleotide T to C polymorphism of IFITM3 associated with increasingly risk of severe and mild influenza in both Asian and Caucasian populations.
The Comeback Kid: Donald Trump on Election Day in 2016
Authors: McKee Seth C. , Smith Daniel A. , Hood M. V. (Trey)
Published: November 2018

The surprise outcome of the 2016 presidential election continues to raise more questions as experts grapple with the evidence for why most prognosticators considered a Hillary Clinton victory almost certain. This article uses the 2016 Cooperative Congressional Election Study data to show that a primary explanation for why the election of Donald Trump was difficult to predict is that the bulk of his support did not materialize until Election Day, in the battleground states that he had to carry to win the Electoral College.
Harms and Wrongs in Epistemic Practice
Authors: Barker Simon , Crerar Charlie , Goetze Trystan S.
Published: November 2018

This volume has its roots in two recent developments within mainstream analytic epistemology: a growing recognition over the past two or three decades of the active and social nature of our epistemic lives; and, more recently still, the increasing appreciation of the various ways in which the epistemic practices of individuals and societies can, and often do, gowrong. The theoretical analysis of these breakdowns in epistemic practice, along with the various harms and wrongs that follow as a consequence, constitutes an approach to epistemology that we refer to asnon-ideal epistemology. In this introductory chapter we introduce and contextualise the ten essays that comprise this volume, situating them within four broad sub-fields: vice epistemology, epistemic injustice, inter-personal epistemic practices, and applied epistemology. We also provide a brief overview of several other important growth areas in non-ideal epistemology.
Just Say ‘No’: Obligations to Voice Disagreement
Authors: Johnson Casey Rebecca
Published: November 2018

It is uncontroversial that we sometimes have moral obligations to voice our disagreements, when, for example, the stakes are high and a wrong course of action will be pursued. But might we sometimes also have epistemic obligations to voice disagreements? In this paper, I will argue that we sometimes do. In other words, sometimes, to be behaving as we ought, qua epistemic agents, we must not only disagree with an interlocutor who has voiced some disagreed-with content but must also testify to this disagreement. This is surprising given that norms on testimony are generally taken to be permissive, and epistemic obligations are usually taken to be negative. In this paper I will discuss some occasions in which epistemic obligations to testify may arise, and I will attempt to investigate the nature of these obligations. I'll briefly discuss the relationship between epistemic and moral norms. I'll offer an account of what it takes to discharge epistemic obligations to testify. Finally, I'll look at some accounts of epistemic obligation that might explain these obligations.
The Epistemology of Terrorism and Radicalisation
Authors: Cassam Quassim
Published: November 2018

This paper outlines and criticises two models of terrorism, the Rational Agent Model (RAM) and the Radicalisation Model (RAD). A different and more plausible conception of the turn to violence is proposed. The proposed account is Moderate Epistemic Particularism (MEP), an approach partly inspired by Karl Jaspers’ distinction between explanation and understanding. On this account there are multiple idiosyncratic pathways to cognitive and behavioural radicalisation, and the actions and motivations of terrorists can only be understood (rather than explained) by engaging with their subjectivity in a way that depends on a degree of empathy. Scepticism is expressed about attempts to model radicalisation and predict political violence. This scepticism is based on reflections concerning the nature of complex particulars. The implications of MEP for counterterrorism are briefly discussed.
Perceived barriers of tobacco dependence treatment: a mixed-methods study among primary healthcare physicians in Armenia
Authors: Harutyunyan Arusyak , Abrahamyan Armine , Hayrumyan Varduhi , Petrosyan Varduhi
Published: November 2018

Background

Despite compelling evidence that physicians play a prominent role in smoking cessation, most smokers do not receive the recommended smoking cessation counseling.

Aim

To identify perceived barriers that hinder primary healthcare physicians (PHPs) from providing smoking cessation treatment to patients in Armenia.

Methods

A sequential exploratory mixed-methods study was conducted among PHPs from two Armenian cities (Yerevan and Gyumri). We implemented qualitative phase through focus group discussions (FGDs) using a semi-structured guide. For the subsequent quantitative phase, the data were collected through cross-sectional survey. A directed deductive content analysis technique was used to analyze the FGDs and questionnaires were analyzed descriptively. Following the data collection (March 2015–May 2016) and descriptive analysis, the qualitative and quantitative data sets were merged by drawing quantitative data onto qualitative categories.

Findings

Overall, 23 PHPs participated in five FGDs and 108 participants completed the survey. Three main categories of barriers were identified: physician-based, patient-based, and system-based barriers. The main physicians-based barriers were insufficient knowledge and inadequate training on tobacco-dependence treatment. Lack of patients’ motivation to quit, poor compliance with the treatment, patients’ withdrawal symptoms were identified as patient-based disincentives. System-based barriers included lack of reimbursement for providing smoking cessation counseling, high price and low availability of smoking cessation medications. Most of the qualitative descriptions were confirmed by quantitative findings.

Conclusions

Targeted interventions are needed to address barriers that limited PHPs’ involvement in providing smoking cessation services in Armenia. There is an urgent need to enhance PHPs’ knowledge and skills in delivering smoking cessation counseling, to increase patients’ demand for smoking cessation services, and to ensure availability and affordability of smoking cessation services in Armenia.
The Anglican Via Media: The Idea of Moderation in Reform
Authors: Holtzen T.L.
Published: November 2018

This essay examines the concept of the Anglican via media and its historical development into its present form. It argues that the Anglican via media is properly understood not as a fixed program of reform, but as moderation in reform, following the classical notion of moderation as a mean between two extremes. The essay traces the theological theme of moderation in reform through the figures of Jewel, Parker, Hooker, Hall, Montagu, Cosin, Forbes, Bramhall, Puller, Knox, to Jebb’s idea of Anglican exceptionalism, and, ultimately, to Newman’s attempt to create the doctrine of the Anglican via media.
Making Gender Visible in Election Violence: Strategies for Data Collection
Authors: Bjarnegård Elin
Published: November 2018

Election violence is an important issue from a number of perspectives. Understanding the causes and consequences of violations of personal integrity is always relevant, but election violence adds a different dimension to this already serious issue: it also violates electoral integrity and decreases democratic quality (Norris 2013). Therefore, election violence should be studied as a simultaneous violation of personal and electoral integrity. In this contribution, I define election violence as occurring when (1) the goal of the act is to affect an electoral outcome or prevent someone from running in an election, and (2) the means by which it is carried out violates the personal integrity of individuals involved in the electoral process.
Stochastic modelling of hydraulic conductivity derived from geotechnical data; an example applied to central Glasgow
Authors: Williams J. D. O. , Dobbs M. R. , Kingdon A. , Lark R. M. , Williamson J. P. , MacDonald A. M. , Ó Dochartaigh B. É.
Published: November 2018

Characterising the three-dimensional (3D) distribution of hydraulic conductivity and its variability in the shallow subsurface is fundamental to understanding groundwater behaviour and to developing conceptual and numerical groundwater models to manage the subsurface. However, directly measuringin situhydraulic conductivity can be difficult and expensive and is rarely carried out with sufficient density in urban environments. In this study we model hydraulic conductivity for 603 sites in the unconsolidated Quaternary deposits underlying Glasgow using particle size distribution and density description widely available from geotechnical investigations. Six different models were applied and the MacDonald formula was found to be most applicable in this heterogeneous environment, comparing well with the few availablein situhydraulic conductivity data. The range of the calculated hydraulic conductivity values between the 5th and 95th percentile was 1.56×10–2–4.38mday–1with a median of 2.26×10–1mday–1. These modelled hydraulic conductivity data were used to develop a suite of stochastic 3D simulations conditioned to existing 3D representations of lithology. Ten per cent of the input data were excluded from the modelling process for use in a split-sample validation test, which demonstrated the effectiveness of this approach compared with non-spatial or lithologically unconstrained models. Our spatial model reduces the mean squared error between the estimated and observed values at the excluded data locations over those predicted using a simple homogeneous model by 73 %. The resulting 3D hydraulic conductivity model is of a much higher resolution than would have been possible from using only direct measurements, and will improve understanding of groundwater flow in Glasgow and reduce the spatial uncertainty of hydraulic parameters in groundwater process models. The methodology employed could be replicated in other regions where significant volumes of suitable geotechnical and site investigation data are available to predict ground conditions in areas with complex superficial deposits.
Geochemistry and related studies of Clyde Estuary sediments
Authors: Jones David G. , Vane Christopher H. , Lass-Evans Solveigh , Chenery Simon , Lister Bob , Cave Mark , Gafeira Joana , Jenkins Gareth , Leslie Alick , Breward Neil , Freeborough Katy , Harrison Ian , Kim Alexander W. , Lacinska Alicja , Milodowski Tony , Ridgway John , Riding Jim , Strutt Mick , Wagner Doris , Wilkinson Ian
Published: November 2018

Geochemical and related studies have been made of near-surface sediments from the River Clyde estuary and adjoining areas, extending from Glasgow to the N, and W as far as the Holy Loch on the W coast of Scotland, UK. Multibeam echosounder, sidescan sonar and shallow seismic data, taken with core information, indicate that a shallow layer of modern sediment, often less than a metre thick, rests on earlier glacial and post-glacial sediments. The offshore Quaternary history can be aligned with onshore sequences, with the recognition of buried drumlins, settlement of muds from quieter water, probably behind an ice dam, and later tidal delta deposits. The geochemistry of contaminants within the cores also indicates shallow contaminated sediments, often resting on pristine pre-industrial deposits at depths less than 1m. The distribution of different contaminants with depth in the sediment, such as Pb (and Pb isotopes), organics and radionuclides, allow chronologies of contamination from different sources to be suggested. Dating was also attempted using microfossils, radiocarbon and 210Pb, but with limited success. Some of the spatial distribution of contaminants in the surface sediments can be related to grain-size variations. Contaminants are highest, both in absolute terms and in enrichment relative to the natural background, in the urban and inner estuary and in the Holy Loch, reflecting the concentration of industrial activity.
Planning the city of tomorrow: bridging the gap between urban planners and subsurface specialists
Authors: Dick Gillian , Eriksson Ingelöv , de Beer Johannes , Bonsor Helen , van der Lugt Petra
Published: November 2018

This paper addresses the process towards the integration of subsurface knowledge into urban planning for three cities – Rotterdam, Glasgow and Oslo – participating in the European Cooperation in Science and Technology (COST) Action TU1206 Sub-Urban. These cities each have unique challenges in managing conflicts and opportunities in the subsurface in the planning process. The COST Sub-Urban Action has enabled a unique interaction between subsurface specialists and urban planners across over 20 European cities, and has laid the foundations for a new understanding between experts who develop subsurface knowledge and those who can benefit most from it – urban planners and decision makers. Common challenges identified include: improving planning policies, enhancing the level of awareness of the subsurface in city development and the modification of legislation to include the subsurface. The paper provides a review of the current status of subsurface planning in the three cities, each of whom are aiming to adapt their urban planning practice and legislation in light of emerging subsurface knowledge, and the current major knowledge gaps. In our opinion, there are two main routes to raise awareness that lead to improved understanding and the use of subsurface information in urban planning processes: (1) the development of a subsurface policy and (2) providing subsurface information. These measures should enable subsurface knowledge to be widely disseminated in order to manage risks and opportunities, and maximise the economic, social and environmental benefits of the urban subsurface and its services on which cities depend.
Wideband filtering power dividers with wide stopband rejection
Authors: Dong Gaoya , Wang Weimin , Zhang Bo , Liu Yuanan
Published: November 2018

A series of novel wideband filtering power dividers (WFPDs) with wide stopband rejection performances is proposed in this paper. The proposed WFPD structure consists of a parallel-coupled line, two transmission line (TL) sections, four loading terminations, and an isolation resistor. The coupled line is applied at the input port to provide wideband impedance transformation, while different types of loading terminations and TL sections are adopted to realize various out-of-band rejection performances. To verify the proposed concepts, three WFPDs operating at 3.0 GHz are designed and fabricated with 3 dB bandwidth of 79.7, 79.0, and 74.4%. In WFPD1, the measured out-of-band rejection of better than 13.4 dB extends to 2.57f0. Moreover, the measured out-of-band rejection of better than 17 dB extends to 2.47f0 (4.75f0) in WFPD2 (WFPD3), respectively. Good agreements between the simulated and measured results validate the presented ideas.
Being born small-for-gestational-age is associated with an unfavourable dietary intake in Danish adolescent girls: findings from the Danish National Birth Cohort
Authors: Kampmann F. B. , Grunnet L. G. , Halldorsson T. I. , Bjerregaard A. A. , Granstrøm C. , Pires S. M. , Strøm M. , Vaag A. A. , Tetens I. , Olsen S. F.
Published: November 2018

Individuals born small have an increased risk for developing type 2 diabetes. Altered food preferences in these subjects seem to play a role; however, limited evidence is available on the association between being born small-for-gestational-age (SGA) at term and food intake in adolescence. Alterations in leptin, ghrelin and dopamine levels are suggested mechanisms linking SGA with later food intake. From a large prospective Danish National Birth Cohort, we compared dietary intake of adolescents being born SGA with normal-for-gestational-age (NGA) adolescents. Intake of foods and nutrients was assessed by a validated food frequency questionnaire in a subsample of 15,607 14-year-old individuals born at term. SGA was defined by birth weight (BW) <10th percentile (n = 1470) and NGA as BW between 10 and 90th percentile (n = 14,137) according to sex and gestational age-specific BW standard curves. Girls born SGA had a 7% (95% CI: 3–12%, P = 0.002) higher intake of added sugar and a 2–8% lower intake of dietary fibre, vegetables, polyunsaturated fatty acids, and total n−6, compared with NGA girls (P < 0.05). Adjusting for parental socio-occupational status, maternal smoking and diet in pregnancy did not substantially change the differences in dietary intake, except from dietary fibre, which were no longer statistically significant. No significant differences in dietary intake between SGA and NGA boys were found. In summary, girls born SGA had an unfavourable dietary intake compared with NGA girls. These differences persisted after controlling for potential confounders, thus supporting a fetal programming effect on dietary intake in girls born SGA at term. However, residual confounding by other factors operating early in childhood cannot be excluded.
Observations and Analysis of the Horizontal Structure of a Tidal Jet at Deep Scour Holes
Authors: Broekema Y. B. , Labeur R. J. , Uijttewaal W. S. J.
Published: November 2018

Scour in the vicinity of a hydraulic structure may compromise its geotechnical stability or that of nearby structures. Much fundamental research has been dedicated to the understanding and prediction of these processes, in order to enable the efficient design of mitigation measures. While most of these efforts consider laterally uniform flows, nonuniformity is the rule rather than the exception. This study presents field observations near the storm surge barrier in the Eastern Scheldt estuary, The Netherlands, where significant scour holes have developed. The tidal flow through this semi‐open barrier exhibits characteristics of a shallow jet. A pronounced contraction of this jet toward the nearby scour hole during particular stages of the tidal cycle is revealed, attributed to potential vorticity conservation; the depth‐averaged vertical vorticity increases proportionally to the depth increase. Measurements show a vertically uniform velocity profile in the scour hole that is attributed to a reduction of the adverse pressure gradient due to the contraction, that suppresses boundary layer separation from the upstream slope of the scour hole. A positive feedback mechanism is thus revealed; lateral velocity gradients lead to relatively high near‐bed velocities in the scour hole which enhances erosion—causing an even stronger horizontal contraction—maintaining the scouring potential. If the upstream flow field is laterally uniform (observed around slack tide), horizontal contraction does not occur, and the boundary layer separates from the upstream slope. The scour depth will then tend to an equilibrium. The results infer the importance of the nonuniformity of the horizontal flow field.
Anomalous Diffusion of Sand Tracer Particles Under Waves
Authors: Wilson Gregory W.
Published: November 2018

A model is presented for the horizontal and vertical diffusion of sand tracer particles by waves and is verified using a reanalysis of the experiments of Miller and Komar (1979, https://doi.org/10.1086/628455). In the model, sand particles take random jumps at a rate associated with the wave period but also can bury one another resulting in intermittent long rest times between jumps. The particle jump length between burials is modeled by considering a vortex entrainment and advection mechanism, resulting in a probability distribution as a function of wave orbital excursion amplitude and particle settling velocity. The model is formalized using the theory of continuous time random walks to obtain closed‐form expressions for the horizontal particle spreading rate, and plume shape, without tunable parameters. Importantly, the model predicts subdiffusive particle spreading, a previously unconsidered phenomenon that is apparent in the experimental data. The plume horizontal extent versus time is also well predicted compared to experimental data.
Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells
Authors: Tamari Keisuke , Konno Masamitsu , Asai Ayumu , Koseki Jun , Hayashi Kazuhiko , Kawamoto Koichi , Murai Noriyuki , Matsufuji Senya , Isohashi Fumiaki , Satoh Taroh , Goto Noriko , Tanaka Shinji , Doki Yuichiro , Mori Masaki , Ogawa Kazuhiko , Ishii Hideshi
Published: November 2018

Cancer stem cells (CSCs) exhibit tumorigenic potential and can generate resistance to chemotherapy and radiotherapy. A labeled ornithine decarboxylase (ODC, a rate-limiting enzyme involved in polyamine [PA] biosynthesis) degradation motif (degron) system allows visualization of a fraction of CSC-like cells in heterogeneous tumor populations. A labeled ODC degradation motif system allowed visualization of a fraction of CSC-like cells in heterogeneous tumor populations. Using this system, analysis of polyamine flux indicated that polyamine metabolism is active in CSCs. The results showed that intracellular polyamines inhibited the activity of histone lysine 4 demethylase enzymes, including lysine-specific demethylase-1 (LSD1). Chromatin immunoprecipitation with Pol II antibody followed by massively parallel DNA sequencing, revealed the global enrichment of Pol II in transcription start sites in CSCs. Increase of polyamines within cells resulted in an enhancement of ID1 gene expression. The results of this study reveal details of metabolic pathways that drive epigenetic control of cancer cell stemness and determine effective therapeutic targets in CSCs.
Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors
Authors: Bulatov Emil , Sayarova Regina , Mingaleeva Rimma , Miftakhova Regina , Gomzikova Marina , Ignatyev Yuri , Petukhov Alexey , Davidovich Pavel , Rizvanov Albert , Barlev Nickolai A.
Published: November 2018

Medicinal bioinorganic chemistry is a thriving field of drug research for cancer treatment. Transition metal complexes coordinated to essential biological scaffolds represent a highly promising class of compounds for design of novel target-specific therapeutics. We report here the biological evaluation of a novel Isatin-Schiff base derivative and its Cu(II) complex in several tumor cell lines by assessing their effects on cellular metabolism, real-time cell proliferation and induction of apoptosis. Further, the impact of compounds on the p53 protein and expression of its target genes, including MDM2, p21/CDKN1A, and PUMA was evaluated. Results obtained in this study provide further evidence in support of our prior data suggesting the p53-mediated mechanism of action for Isatin-Schiff base derivatives and their complexes and also shed light on potential use of these compounds for stimulation of apoptosis in breast cancer cells via activation of the pro-apoptotic PUMA gene.
The evolutionary dynamics of microRNAs in domestic mammals
Authors: Penso-Dolfin Luca , Moxon Simon , Haerty Wilfried , Di Palma Federica
Published: November 2018

MiRNAs are crucial regulators of gene expression found across both the plant and animal kingdoms. While the number of annotated miRNAs deposited in miRBase has greatly increased in recent years, few studies provided comparative analyses across sets of related species, or investigated the role of miRNAs in the evolution of gene regulation. We generated small RNA libraries across 5 mammalian species (cow, dog, horse, pig and rabbit) from 4 different tissues (brain, heart, kidney and testis). We identified 1676 miRBase and 413 novel miRNAs by manually curating the set of computational predictions obtained from miRCat and miRDeep2. Our dataset spanning five species has enabled us to investigate the molecular mechanisms and selective pressures driving the evolution of miRNAs in mammals. We highlight the important contributions of intronic sequences (366 orthogroups), duplication events (135 orthogroups) and repetitive elements (37 orthogroups) in the emergence of new miRNA loci. We use this framework to estimate the patterns of gains and losses across the phylogeny, and observe high levels of miRNA turnover. Additionally, the identification of lineage-specific losses enables the characterisation of the selective constraints acting on the associated target sites. Compared to the miRBase subset, novel miRNAs tend to be more tissue specific. 20 percent of novel orthogroups are restricted to the brain, and their target repertoires appear to be enriched for neuron activity and differentiation processes. These findings may reflect an important role for young miRNAs in the evolution of brain expression plasticity. Many seed sequences appear to be specific to either the cow or the dog. Analyses on the associated targets highlight the presence of several genes under artificial positive selection, suggesting an involvement of these miRNAs in the domestication process. Altogether, we provide an overview on the evolutionary mechanisms responsible for miRNA turnover in 5 domestic species, and their possible contribution to the evolution of gene regulation.
Awareness, treatment and control of hypertension among hypertensive patients aged 18 to 59 years old in the northeast of China
Authors: Lv Xin , Niu Huikun , Qu Yangming , Li Meiqi , Li Lu , Ma Xiaoyu , Jiang Shan , Gao Chunshi , Wang Rui , Zhang Peng , Li Bo
Published: November 2018

Hypertension not only has a high prevalence, but also brings disease burden to the affected patients. To assess the level of awareness, treatment and control of hypertension in the northeast of China, we investigated the rates of awareness, treatment and control of hypertension and identified its related factors among hypertensive patients aged 18 to 59 years old in Jilin, China. The data (n = 4632) for the present study were extracted from a cross-sectional study in Jilin. Among individuals with hypertension, the standardized rates of awareness, treatment and control of hypertension were 44.9%, 36.5%, 24.3%, respectively. The rates of awareness and treatment of hypertension among middle aged patients were higher than those among young patients. Compared to patients with normal Body Mass index (BMI), obese hypertensive patients had a higher rate of treatment (43.7% vs. 25.9%) and a lower rate of control (18.9% vs. 29.6%). Compared to patients with normal BMI, patients who were obese were more likely to take measures to treat hypertension (OR = 2.50, 95%CI: 2.05–3.05); but were less likely to have well-controlled blood pressure (OR = 0.55, 95%CI: 0.40–0.78). BMI is one of the influencing factors of hypertension awareness, treatment and control among patients 18 to 59 years old with hypertension.
Spatio-temporal cluster and distribution of human brucellosis in Shanxi Province of China between 2011 and 2016
Authors: Wang Ting , Wang Xiang , Tie Ping , Bai Yongfei , Zheng Yuhua , Yan Changfu , Chai Zhikai , Chen Jing , Rao Huaxiang , Zeng Lingjia , Chen Limin , Qiu Lixia
Published: November 2018

In recent years, the incidence of human brucellosis (HB) in the Shanxi province has ranked to be the top five among the 31 China provinces. HB data in Shanxi province between 2011 and 2016 were collected from the Centers for Disease Control and Prevention. Spatial and temporal distribution of HB was evaluated using spatial autocorrelation analysis and space-time scan analysis. The global Moran’s I index ranged from 0.37 to 0.50 between 2011 and 2016 (all P < 0.05), and the “high-high” clusters of HB were located at the northern Shanxi, while the “low-low” clusters in the central and southeastern Shanxi. The high-incidence time interval was between March and July with a 2-fold higher risk of HB compared to the other months in the same year. One most likely cluster and three secondary clusters were identified. The radius of the most likely cluster region was 158.03 km containing 10,051 HB cases. Compared to the remaining regions, people dwelling in the most likely region were reported 4.50-fold ascended risk of incident HB. HB cases during the high-risk time interval of each year were more likely to be younger, to be males or to be farmers or herdsman than that during the low-risk time interval. The HB incidence had a significantly high correlation with the number of the cattle or sheep especially in the northern Shanxi. HB in Shanxi showed unique spatio-temporal clustering. Public health concern for HB in Shanxi should give priority to the northern region especially between the late spring and early summer.
Lipoprotein markers associated with disability from multiple sclerosis
Authors: Gafson A. R. , Thorne T. , McKechnie C. I. J. , Jimenez B. , Nicholas R. , Matthews P. M.
Published: November 2018

Altered lipid metabolism is a feature of chronic inflammatory disorders. Increased plasma lipids and lipoproteins have been associated with multiple sclerosis (MS) disease activity. Our objective was to characterise the specific lipids and associated plasma lipoproteins increased in MS and to test for an association with disability. Plasma samples were collected from 27 RRMS patients (median EDSS, 1.5, range 1–7) and 31 healthy controls. Concentrations of lipids within lipoprotein sub-classes were determined from NMR spectra. Plasma cytokines were measured using the MesoScale Discovery V-PLEX kit. Associations were tested using multivariate linear regression. Differences between the patient and volunteer groups were found for lipids within VLDL and HDL lipoprotein sub-fractions (p < 0.05). Multivariate regression demonstrated a high correlation between lipids within VLDL sub-classes and the Expanded Disability Status Scale (EDSS) (p < 0.05). An optimal model for EDSS included free cholesterol carried by VLDL-2, gender and age (R2 = 0.38, p < 0.05). Free cholesterol carried by VLDL-2 was highly correlated with plasma cytokines CCL-17 and IL-7 (R2 = 0.78, p < 0.0001). These results highlight relationships between disability, inflammatory responses and systemic lipid metabolism in RRMS. Altered lipid metabolism with systemic inflammation may contribute to immune activation.
The efficacy of serum cell death biomarkers for diagnosing biliary tract cancer
Authors: Sugimoto Mitsuru , Abe Kazumichi , Hayashi Manabu , Takagi Tadayuki , Suzuki Rei , Konno Naoki , Asama Hiroyuki , Sato Yuki , Irie Hiroki , Watanabe Ko , Nakamura Jun , Kikuchi Hitomi , Waragai Yuichi , Takasumi Mika , Hashimoto Minami , Hikichi Takuto , Nozawa Yoshihiro , Ohira Hiromasa
Published: November 2018

In this study, we determined the efficacy of the cell death biomarker cytokeratin 18 for diagnosing biliary tract cancer (BTC). We recruited 36 patients with BTC (Malignant group) and 45 patients with benign biliary tract disease (Benign group) for this study. We used M30 and M65 as cell death biomarkers. M30 levels indicate apoptosis, and M65 levels indicate both apoptosis and necrosis. M30 and M65 levels were significantly higher in the Malignant group than in the Benign group (142.4 ± 117.0 vs 48.9 ± 71.2 U/l, P < 0.001; 1513.3 ± 837.4 vs 882.2 ± 831.2 U/l, P = 0.001). The diagnosability of M30 was the highest of the four markers (CEA, CA19-9, M30, M65) (cut-off value: 74.429 U/l, sensitivity: 72.2%, specificity: 77.1%, AUC: 0.771). The sensitivity of M30 (cut-off value: 74.429 U/l) was significantly higher than that of biliary cytology (76% (19/25) vs 12% (3/25), P < 0.001), and the accuracy of M30 was significantly higher than that of biliary cytology (78.3% (36/46) vs 52.2% (24/46), P = 0.015). The sensitivity of M30 (cut-off value: 74.429 U/l) was significantly higher than that of biliary cytology and brush cytology (72.4% (21/29) vs 24.1% (7/29), P < 0.001). In conclusion, cell death biomarkers were increased in patients with BTC, and M30 could efficiently diagnose BTC.
Physical mechanisms of oceanic mantle earthquakes: Comparison of natural and experimental events
Authors: Kita Saeko , Ferrand Thomas P.
Published: November 2018

Because they provide information about the spatial distribution of brittle deformation, both seismologists and experimentalists use b-values to study earthquake populations. Here, we present the b-values for intermediate-depth intraslab earthquakes in the Pacific slab beneath the Tohoku and Hokkaido regions, northeastern Japan and find a difference in the lower-plane event b-values in the double seismic zone. Lower-plane events reveal significantly larger b-values beneath Tohoku (0.96) than Hokkaido (0.86), implying that the brittle deformation beneath Hokkaido is more localized and leads to higher ratio of relatively large lower-plane events than occur beneath Tohoku. We also estimated the b-values for experimental earthquakes, and found they increase with increasing antigorite content in serpentinized peridotite. These experimental earthquakes already led to the “dehydration driven stress transfer” (DDST) model, which suggests that a highly hydrated peridotite is not required when oceanic mantle events occur. A comparison of experimental and natural earthquake b-values implies that lower-plane peridotite is more hydrated beneath the Tohoku region, which could also explain the difference in oceanic-plate velocity structures near the trench identified in Ocean Bottom Seismometer studies off Tohoku and Hokkaido. These results suggest that lower-plane events occur in fresh peridotite near serpentinized faults.
Detection of correlated hidden factors from single cell transcriptomes using Iteratively Adjusted-SVA (IA-SVA)
Authors: Lee Donghyung , Cheng Anthony , Lawlor Nathan , Bolisetty Mohan , Ucar Duygu
Published: November 2018

Single cell RNA-sequencing (scRNA-seq) precisely characterizes gene expression levels and dissects variation in expression associated with the state (technical or biological) and the type of the cell, which is averaged out in bulk measurements. Multiple and correlated sources contribute to gene expression variation in single cells, which makes their estimation difficult with the existing methods developed for batch correction (e.g., surrogate variable analysis (SVA)) that estimate orthogonal transformations of these sources. We developed iteratively adjusted surrogate variable analysis (IA-SVA) that can estimate hidden factors even when they are correlated with other sources of variation by identifying a set of genes associated with each hidden factor in an iterative manner. Analysis of scRNA-seq data from human cells showed that IA-SVA could accurately capture hidden variation arising from technical (e.g., stacked doublet cells) or biological sources (e.g., cell type or cell-cycle stage). Furthermore, IA-SVA delivers a set of genes associated with the detected hidden source to be used in downstream data analyses. As a proof of concept, IA-SVA recapitulated known marker genes for islet cell subsets (e.g., alpha, beta), which improved the grouping of subsets into distinct clusters. Taken together, IA-SVA is an effective and novel method to dissect multiple and correlated sources of variation in scRNA-seq data.