bluuebunny/crossref_metadata_2025 huggingface dataset:
Operative Workflow from CT to 3D Printing of the Heart: Opportunities and Challenges
Authors: Bertolini Michele , Rossoni Marco , Colombo Giorgio
Published: September 2021

Medical images do not provide a natural visualization of 3D anatomical structures, while 3D digital models are able to solve this problem. Interesting applications based on these models can be found in the cardiovascular field. The generation of a good-quality anatomical model of the heart is one of the most complex tasks in this context. Its 3D representation has the potential to provide detailed spatial information concerning the heart’s structure, also offering the opportunity for further investigations if combined with additive manufacturing. When investigated, the adaption of printed models turned out to be beneficial in complex surgical procedure planning, for training, education and medical communication. In this paper, we will illustrate the difficulties that may be encountered in the workflow from a stack of Computed Tomography (CT) to the hand-held printed heart model. An important goal will consist in the realization of a heart model that can take into account real wall thickness variability. Stereolithography printing technology will be exploited with a commercial rigid resin. A flexible material will be tested too, but results will not be so satisfactory. As a preliminary validation of this kind of approach, print accuracy will be evaluated by directly comparing 3D scanner acquisitions to the original Standard Tessellation Language (STL) files.
Nephroprotective Role of Chrysophanol in Hypoxia/Reoxygenation-Induced Renal Cell Damage via Apoptosis, ER Stress, and Ferroptosis
Authors: Lin Chih-Hung , Tseng Han-Fang , Hsieh Po-Chun , Chiu Valeria , Lin Ting-Yun , Lan Chou-Chin , Tzeng I-Shiang , Chao Huan-Nung , Hsu Chia-Chen , Kuo Chan-Yen
Published: September 2021

Acute kidney injury (AKI) is caused by hypoxia-reoxygenation (H/R), which is a kidney injury produced by a variety of causes, resulting in the remaining portion of the kidney function being unable to maintain the balance for performing the tasks of waste excretion metabolism, and electrolyte and acid-base balance. Many studies have reported the use of Chinese medicine to slow down the progression and alleviate the complications of chronic renal failure. Chrysophanol is a component of Rheum officinale Baill, a traditional Chinese medicine that has been clinically used to treat renal disease. We aimed to study the nephroprotective effect of chrysophanol on hypoxia/ reoxygenation (H/R)-induced cell damage. The results showed that chrysophanol prevented H/R-induced apoptosis via downregulation of cleaved Caspase-3, p-JNK, and Bax but upregulation of Bcl-2 expression. In contrast, chrysophanol attenuated H/R-induced endoplasmic reticulum (ER) stress via the downregulation of CHOP and p-IRE1α expression. Our data demonstrated that chrysophanol alleviated H/R-induced lipid ROS accumulation and ferroptosis. Therefore, we propose that chrysophanol may have a protective effect against AKI by regulating apoptosis, ER stress, and ferroptosis.
Clinical Outcome of RAMPS for Left-Sided Pancreatic Ductal Adenocarcinoma: A Comparison of Anterior RAMPS versus Posterior RAMPS for Patients without Periadrenal Infiltration
Authors: Kwon Jaewoo , Park Yejong , Jun Eunsung , Lee Woohyung , Song Ki Byung , Lee Jae Hoon , Hwang Dae Wook , Kim Song Cheol
Published: September 2021

Radical antegrade modular pancreatosplenectomy (RAMPS) is considered an effective procedure for left-sided pancreatic ductal adenocarcinoma (PDAC). However, whether there are differences in perioperative outcomes, pathologies, or survival outcomes between anterior RAMPS (aRAMPS) and posterior RAMPS (pRAMPS) has not been reported previously. We retrospectively reviewed and compared the demographic, perioperative, histopathologic, and survival data of patients who underwent aRAMPS or pRAMPS for PDAC. We also compared these two groups among patients without periadrenal infiltration or adrenal invasion. A total of 112 aRAMPS patients and 224 pRAMPS patients were evaluated. Periadrenal infiltration, neoadjuvant treatment, and concurrent vessel resection were more prevalent in the pRAMPS group. After excluding patients with periadrenal infiltration, 106 aRAMPS patients were compared with 157 pRAMPS patients. There were no significant differences between the aRAMPS and pRAMPS groups in the pathologic tumor size, resection margin, proportion of tangential margin in the R1 resection, and number of harvested lymph nodes. The median overall survival and disease-free survival also did not differ significantly between the two groups. We cautiously suggest that pRAMPS will not necessarily provide more beneficial histopathologic outcomes and survival rates for left-sided PDAC cases without periadrenal infiltration. If periadrenal infiltration is not suspected, aRAMPS alone should be sufficiently effective.
Conformational Analysis of [60]PCBM from DFT Simulations of Electronic Energies, Bond Strain and the 13C NMR Spectrum: Input Geometry Determination and Ester Bond Rotation Dynamics
Authors: Liu Tong , Dennis T. John S.
Published: September 2021

With the aim of determining the best input geometry for DFT calculations of [60]PCBM, the geometry of 24 chemically possible [60]PCBM conformers were optimised and their electronic energies and average bond strains were determined. A DFT analysis of the relevant dihedral angles provided insights into the dynamical behaviour of the ester group through sterically restricted bond rotations. In addition, the 13C NMR spectra of the six better performing conformers were simulated and compared with an experiment. There is a close correlation between average bond strain, total electronic energy and mean absolute error of the simulated 13C NMR spectra of the ester carbons. The best overall candidate conformer for the input geometry had the C61-C4, C4-C3 and C3-C2 single bonds of the alkyl chain in syn, anti and anti arrangements, respectively, and had the C2-C1 and C1-O single bonds of the ester in syn and anti arrangements, respectively. This contrasts strikingly with most representations of PCBM in the literature, which depict all relevant bonds in anti arrangements.
The Role of Extracellular HSP70 in the Function of Tumor-Associated Immune Cells
Authors: Linder Manuel , Pogge von Strandmann Elke
Published: September 2021

Extracellular vesicles released by tumor cells (T-EVs) are known to contain danger-associated molecular patterns (DAMPs), which are released in response to cellular stress to alert the immune system to the dangerous cell. Part of this defense mechanism is the heat shock protein 70 (HSP70), and HSP70-positive T-EVs are known to trigger anti-tumor immune responses. Moreover, extracellular HSP70 acts as an immunogen that contributes to the cross-presentation of major histocompatibility complex (MHC) class I molecules. However, the release of DAMPs, including HSP70, may also induce chronic inflammation or suppress immune cell activity, promoting tumor growth. Here, we summarize the current knowledge on soluble, membrane-bound, and EV-associated HSP70 regarding their functions in regulating tumor-associated immune cells in the tumor microenvironment. The molecular mechanisms involved in the translocation of HSP70 to the plasma membrane of tumor cells and its release via exosomes or soluble proteins are summarized. Furthermore, perspectives for immunotherapies aimed to target HSP70 and its receptors for cancer treatment are discussed and presented.
Prostate Health Index and Multiparametric MRI: Partners in Crime Fighting Overdiagnosis and Overtreatment in Prostate Cancer
Authors: Ferro Matteo , Crocetto Felice , Bruzzese Dario , Imbriaco Massimo , Fusco Ferdinando , Longo Nicola , Napolitano Luigi , La Civita Evelina , Cennamo Michele , Liotti Antonietta , Lecce Manuela , Russo Gianluca , Insabato Luigi , Imbimbo Ciro , Terracciano Daniela
Published: September 2021

Widespread use of PSA as the standard tool for prostate cancer (PCa) diagnosis led to a high rate of overdiagnosis and overtreatment. In this study, we evaluated the performance of the prostate health index (PHI) and multiparametric magnetic resonance imaging (mpMRI) for the prediction of positive biopsy and of high-grade PCa at radical prostatectomy (RP). To this end, we prospectively enrolled 196 biopsy-naïve patients who underwent mpMRI. A subgroup of 116 subjects with biopsy-proven PCa underwent surgery. We found that PHI significantly outperformed both PI-RADS score (difference in AUC: 0.14; p < 0.001) and PHI density (difference in AUC: 0.08; p = 0.002) in the ability to predict positive biopsy with a cut-off value of 42.7 as the best threshold. Conversely, comparing the performance in the identification of clinically significant prostate cancer (csPCa) at RP, we found that PHI ≥ 61.68 and PI-RADS score ≥ 4 were able to identify csPCa (Gleason score ≥ 7 (3 + 4)) both alone and added to a base model including age, PSA, fPSA-to-tPSA ratio and prostate volume. In conclusion, PHI had a better ability than PI-RADS score to predict positive biopsy, whereas it had a comparable performance in the identification of pathological csPCa.
Meta-Analysis of Isolated Hepatic Perfusion and Percutaneous Hepatic Perfusion as a Treatment for Uveal Melanoma Liver Metastases
Authors: Bethlehem Martijn S. , Katsarelias Dimitrios , Olofsson Bagge Roger
Published: September 2021

Background: Uveal melanoma is the most commonly occurring primary intraocular malignancy in adults, and patients have a high risk of developing metastatic disease, mostly in the liver. Isolated hepatic perfusion (IHP) with melphalan is a liver-directed therapy for patients with liver metastases. Percutaneous hepatic perfusion (PHP), a minimally invasive technique, is available as well. PHP benefits from the fact that the procedure can be repeated and therefore possibly offers better survival. We conducted a systematic review and meta-analysis comparing both techniques. Methods: A systematic literature search was performed using the electronic databases of Scopus, MEDLINE, Web of Science, PubMed and Cochrane CENTRAL. A total of nine articles reporting on eight studies were included in the analysis. Individual survival data were extracted from each study. Results: The median overall survival (OS) was 17.1 months for IHP and 17.3 months for PHP. The median progression-free survival (PFS) was 7.2 months for IHP and 9.6 months for PHP. The median hepatic progression-free survival was 10 months for IHP and 9.5 months for PHP. The complication rate and 30-day mortality rate were 39.1% and 5.5% for IHP and 23.8% and 1.8% for PHP. Conclusion: There was no difference in OS or PFS between IHP and PHP for patients with uveal melanoma liver metastases, but patients have significantly less of a risk for complications and mortality following PHP.
Graphene Oxide Synthesis, Properties and Characterization Techniques: A Comprehensive Review
Authors: Trikkaliotis Dimitrios G. , Christoforidis Achilleas K. , Mitropoulos Athanasios C. , Kyzas George Z.
Published: September 2021

The unique properties of graphene oxide (GO) have attracted the attention of the research community and cost-effective routes for its production are studied. The type and percentage of the oxygen groups that decorate a GO sheet are dependent on the synthesis path, and this path specifies the carbon content of the sheet. The chemical reduction of GO results in reduced graphene oxide (rGO) while the removal of the oxygen groups is also achievable with thermal processes (tpGO). This review article introduces the reader to the carbon allotropes, provides information about graphene which is the backbone of GO and focuses on GO synthesis and properties. The last part covers some characterization techniques of GO (XRD, FTIR, AFM, SEM-EDS, N2 porosimetry and UV-Vis) with a view to the fundamental principles of each technique. Some critical aspects arise for GO synthesized and characterized from our group.
A Prospective Cohort Study on Quality of Life among the Pediatric Population after Surgery for Recurrent Patellar Dislocation
Authors: Herdea Alexandru , Pencea Vlad , Lungu Claudiu N. , Charkaoui Adham , Ulici Alexandru
Published: September 2021

Patellofemoral instability is a frequent cause of knee pathology affecting quality of life among the pediatric population. Here, we present a prospective cohort study which included patients who had undergone surgical management using the lateral release and medial imbrication approach (LRMI) or medial patellofemoral ligament reconstruction (MPFL-R). The object of this study was to assess the quality of life among children that have undergone surgical treatment for patellar dislocation. Quality of life was assessed before and after surgery using the Pediatric International Knee Documentation Committee form (Pedi-IKDC), a questionnaire that aims to quantify knee functionality. Postoperative scarring was evaluated using The Stony Brook Scar Evaluation Scale. One hundred and eight patients were selected and grouped according to the type of procedure. Before surgery, the two groups had similar mean Pedi-IKDC scores (41,4 MPFL-R vs. 39,4 LRMI p = 0.314). Improvements were observed in the postoperative scores. The MPFL-R technique showed promising outcomes. When comparing the two surgical groups, there was a significant difference in favor of MPFL-R group (MPFL-R 77.71 points vs. LRMI 59.74 points, p < 0.0001–95% CI (11.22–24.72)). Using the Stony Brook Scar Evaluation Scale, a significant difference in scar quality in favor of MPFL-R was observed (4,5 MPFL-R vs. 2,77 LRMI p = 0.002). In conclusion, this study provides objective evidence-based outcome assessments that support the medial patellofemoral ligament reconstruction technique as the gold standard for patellofemoral instability.
A Population Based Study of Liver Function amongst Adults with Hyperuricemia and Gout in the United States
Authors: Deb Subrata , Sakharkar Prashant
Published: September 2021

To examine the association between uric acid levels and liver enzyme functions amongst adults with hyperuricemia and gout in the United States. The National Health and Nutrition Examination Survey (NHANES) data from 2007 to 2016 was used to study the research objective. Data were analyzed for descriptive statistics and for differences using the t test, Chi-square test and ANOVA. A regression analysis was performed to determine association between demographics and liver enzymes. A p value of <0.05 or <0.001 was considered statistically significant. A total of 14,946 adults (≥20 yrs.) were included in this study. Sample mean age was 49 ± 0.15 yrs., and 54% were female. Overall, 15% adults had elevated uric acid levels (≥6.8 mg/dL), men had significantly higher uric acid levels than women (6 mg/dL vs. 4.8 mg/dL). High uric acid levels were associated with more than two times higher odds of elevated ALT, AST and GGT (p < 0.001). Similarly, gender-based target uric acid values were associated with two-fold increased odds of GGT, over one-and-a-half fold higher odds of ALT and AST (p < 0.001). Regression analysis showed significant association between age, gender, race/ethnicity, body mass index, and hypertension and ALT, AST, ALP, total bilirubin and GGT (p < 0.001). Adults with hyperuricemia and gout are most likely to develop liver dysfunctions and suffer associated morbidities. Such patients need to be appropriately monitored and managed for their liver functions and to prevent associated morbidities.
A Review of How Building Mitigates the Urban Heat Island in Indonesia and Tropical Cities
Authors: Sari Dany Perwita
Published: September 2021

A consequence of urbanization was the intensification of urban heat islands, especially in tropical cities. There have been rapid developments in infrastructure that have displaced open spaces. Meanwhile, Indonesia has a tropical climate directly affected by climate change. A high priority has been placed on adaptation measures to address issues such as sea-level rise, increased extreme weather, and threats to ecosystems and biodiversity. There is still a lack of specific knowledge regarding tropical climate in urban areas. In this paper, the author examines how building and urban planning affect urban heat islands in the tropics. According to the review, early planning and building based on local weather data can reduce the energy consumption and minimize the UHI effect. Furthermore, a media campaign and early education should increase awareness about adaptation measures between governments and citizens. Based on the findings from this study, some recommendations are offered for future urban planning, especially for tropical climates, to reduce UHI effects.
Response of Biodiversity, Ecosystems, and Ecosystem Services to Climate Change in China: A Review
Authors: Yang Haijiang , Gou Xiaohua , Yin Dingcai
Published: September 2021

Climate change is having a significant impact on the global ecosystem and is likely to become increasingly important as this phenomenon intensifies. Numerous studies in climate change impacts on biodiversity, ecosystems, and ecosystem services in China have been published in recent decades. However, a comprehensive review of the topic is needed to provide an improved understanding of the history and driving mechanisms of environmental changes within the region. Here we review the evidence for changes in climate and the peer-reviewed literature that assesses climate change impacts on biodiversity, ecosystem, and ecosystem services at a China scale. Our main conclusions are as follows. (1) Most of the evidence shows that climate change (the increasing extreme events) is affecting the change of productivity, species interactions, and biological invasions, especially in the agro-pastoral transition zone and fragile ecological area in Northern China. (2) The individuals and populations respond to climate change through changes in behavior, functions, and geographic scope. (3) The impact of climate change on most types of services (provisioning, regulating, supporting, and cultural) in China is mainly negative and brings threats and challenges to human well-being and natural resource management, therefore, requiring costly societal adjustments. In general, although great progress has been made, the management strategies still need to be further improved. Integrating climate change into ecosystem services assessment and natural resource management is still a major challenge. Moving forward, it is necessary to evaluate and research the effectiveness of typical demonstration cases, which will contribute to better scientific management of natural resources in China and the world.
Thermoelectric Power Generators: State-of-the-Art, Heat Recovery Method, and Challenges
Authors: Aridi Rima , Faraj Jalal , Ali Samer , Lemenand Thierry , Khaled Mahmoud
Published: September 2021

Electricity plays a significant role in daily life and is the main component of countless applications. Thus, ongoing research is necessary to improve the existing approaches, or find new approaches, to enhancing power generation. The thermoelectric generator (TEG) is among the notable and widespread technologies used to produce electricity, and converts waste energy into electrical energy using the Seebeck effect. Due to the Seebeck effect, temperature change can be turned into electrical energy; hence, a TEG can be applied whenever there is a temperature difference. The present paper presents the theoretical background of the TEG, in addition to a comprehensive review of the TEG and its implementation in various fields. This paper also sheds light on the new technologies of the TEG and their related challenges. Notably, it was found that the TEG is efficient in hybrid heat recovery systems, such as the phase change material (PCM), heat pipe (HP), and proton exchange membrane (PEM), and the efficiency of the TEG has increased due to a set of improvements in the TEG’s materials. Moreover, results show that the TEG technology has been frequently applied in recent years, and all of the investigated papers agree that the TEG is a promising technology in power generation and heat recovery systems.
Criteria, Indicators, and Factors of the Sustainable Energy-Saving Economic Development: The Case of Natural Gas Consumption
Authors: Yemelyanov Olexandr , Symak Anastasiya , Petrushka Tetyana , Vovk Olena , Ivanytska Oksana , Symak Dmytro , Havryliak Anatolii , Danylovych Taras , Lesyk Lilia
Published: September 2021

To solve the contradiction between achieving long-term economic growth and reducing the consumption of certain types of resources, the concept of sustainable resource saving economic development must be put into practice. The purpose of this research is to establish criteria, develop indicators, and identify factors of the sustainable energy-saving economic development, as well as to test the developed theoretical provisions using the example of natural gas consumption by different countries. To achieve this goal, various methods were used, including economic and mathematical modeling, time series analysis, factor analysis, regression analysis, and so on. The criteria were formalized, according to which a certain type of economic development can be attributed to energy saving both at the level of the state economy as a whole and at the level of individual industries and enterprises. It was established that the formalized criteria of the sustainable energy-saving economic development have the form of chains of inequalities, and their application makes it possible to identify the general conditions for ensuring this type of development. The main properties of energy-saving economic development were identified. They include the pace of this development, its potential, balance, permanence, and other characteristics. Indicators that can be used to quantify these characteristics were developed. The factors influencing the scale and time characteristics of sustainable energy-saving economic development at the level of the state economy and that of industries and individual enterprises, were systematized. The dynamics of natural gas consumption in different countries was analyzed. The reasons for the lack of energy-saving natural gas economic development in some countries were identified. A quantitative assessment of the properties of this type of economic development by country was conducted. The influence of some factors on the parameters of the sustainable energy-saving natural gas economic development of countries was analyzed. The existence of a negative effect of the rebound in the consumption of natural gas was established at certain intervals in some countries. The obtained results provide an opportunity to increase the degree of understanding of the complex patterns that underlie the sustainable energy-saving economic development of states, industries, and enterprises. These results can also be used in the development of government programs to stimulate energy conservation.
Simulation and Sensitivity Analysis for Various Geometries and Optimization of Solid Oxide Fuel Cells: A Review
Authors: Tonekabonimoghaddam Mina , Shamiri Ahmad
Published: September 2021

Solid oxide fuel cells (SOFCs) are considered as one of the most promising fuel cell types for application as high efficiency power generators. This work reviews the use of computational fluid dynamics (CFD) to maximise SOFC performance and life, and minimise cost, by considering numerous configurations and designs. A critical analysis of available literature proves that detailed research on the simulation of thermal stress and its damaging impact on the SOFC is still in its early stage of development. Numerical simulation is expected to help optimize the design, operating parameters and fuel cell materials. Therefore, sensitivity analysis of fuel cell parameters using simulation models is analysed to address the issue. Finally, the present status of the SOFC optimization efforts is summarized so that unresolved problems can be identified and solved.
Residual Gas for Ethanol Production by Clostridium carboxidivorans in a Dual Impeller Stirred Tank Bioreactor (STBR)
Authors: Benevenuti Carolina , Branco Marcelle , do Nascimento-Correa Mariana , Botelho Alanna , Ferreira Tatiana , Amaral Priscilla
Published: September 2021

Recycling residual industrial gases and residual biomass as substrates to biofuel production by fermentation is an important alternative to reduce organic wastes and greenhouse gases emission. Clostridium carboxidivorans can metabolize gaseous substrates as CO and CO2 to produce ethanol and higher alcohols through the Wood-Ljungdahl pathway. However, the syngas fermentation is limited by low mass transfer rates. In this work, a syngas fermentation was carried out in serum glass bottles adding different concentrations of Tween® 80 in ATCC® 2713 culture medium to improve gas-liquid mass transfer. We observed a 200% increase in ethanol production by adding 0.15% (v/v) of the surfactant in the culture medium and a 15% increase in biomass production by adding 0.3% (v/v) of the surfactant in the culture medium. The process was reproduced in stirred tank bioreactor with continuous syngas low flow, and a maximum ethanol productivity of 0.050 g/L.h was achieved.
A Mixed Methods Literature Review and Framework for Decision Factors That May Influence the Utilization of Managed Wildfire on Federal Lands, USA
Authors: Fillmore Stephen D. , McCaffrey Sarah M. , Smith Alistair M. S.
Published: September 2021

There is increasing discussion in the academic and agency literature, as well as popular media, about the need to address the existing deficit of beneficial fire on landscapes. One approach allowable under United States federal wildland fire policy that could help address this condition is by deliberately managing wildfire with a strategy other than full suppression (hereafter referred to as ‘managed wildfire’). To improve the understanding of the managed fire decision-making process, we conducted a mixed methods review of the existing literature. This review spanned 1976 to 2013 and used thematic coding to identify key factors that affect the decision to manage a wildfire. A total of 110 descriptive factors categories were identified. These were classified into six key thematic groups, which addressed specific decision considerations. This nexus of factors and decision pathways formed what we describe as the ‘Managed Fire Decision Framework’, which contextualizes important pressures, barriers, and facilitators related to managed wildfire decision-making. The most prevalent obstacles to managing wildfire were operational concerns and risk aversion. The factor most likely to support managing a fire was the decision maker’s desire to see the strategy be implemented. Ultimately, we found that the managed fire decision-making process is extremely complex, and that this complexity may itself be a barrier to its implementation.
Comparative Analysis of the nrDNA Repeat Unit of Manila Clam Ruditapes philippinarum and Quahog Mercenaria mercenaria
Authors: Guo Zhansheng , Wang Zhen , Hou Xuguang
Published: September 2021

Ruditapes philippinarum and Mercenaria mercenaria are economically important bivalve species. The complete ribosomal DNA (rDNA) unit sequences of R. philippinarum and M. mercenaria, with as-sembled rDNA unit lengths of 12,910 and 12,100 bp, respectively, were obtained in this study for the first time. The rDNA unit structural organisation was similar to that in other eukaryotes, in-cluding the following elements in order: 18S rRNA-internal transcribed spacer 1 (ITS1); 5.8S rRNA-ITS2-28S rRNA-intergenic spacer (IGS) (3′ external transcribed spacer (ETS); non-transcribed spacer (NTS)-5′ ETS). The genetic differences between R. philippinarum and M. mercenaria were mainly attributable to non-coding regions (ITS1, ITS2 and IGS), especially the IGS region. The boundaries of putative 3′ ETS, NTS and 5′ ETS were confirmed. Seven and three sub-repeat fragments were found in R. philippinarum and M. mercenaria, respectively. These frag-ments ranged from 4 to 154 bp in length, and were located at the NTS and 5′ ETS regions. Five and six cytosine–guanine (CpG) islands were detected in R. philippinarum and M. mercenaria, respec-tively, and these covered 85.58% and 79.29% of the entire IGS sequence, respectively. The phylo-genetic tree was constructed based on Veneridae ITS and 18S rRNA sequences using the maxi-mum likelihood (ML) method. The ML tree based on ITS revealed that species within the same genus clearly clustered together with relatively high supporting values, and all the genera were recovered as monophyletic. The phylogenetic analyses using 18S rRNA provided a weaker phy-logenetic signal than ITS.
Health-Promoting Properties of Lacticaseibacillus paracasei: A Focus on Kefir Isolates and Exopolysaccharide-Producing Strains
Authors: Bengoa Ana Agustina , Dardis Carolina , Garrote Graciela L. , Abraham Analía G.
Published: September 2021

Among artisanal fermented beverages, kefir (fermented milk drink) and water kefir (fermented nondairy beverage) are of special interest because their grains can be considered natural reservoirs of safe and potentially probiotic strains. In the last years, several reports on Lacticaseibacillus paracasei (formerly Lactobacillus paracasei) isolated from both artisanal fermented beverages were published focusing on their health-promoting properties. Although this is not the predominant species in kefir or water kefir, it may contribute to the health benefits associated to the consumption of the fermented beverage. Since the classification of L. paracasei has been a difficult task, the selection of an adequate method for identification, which is essential to avoid mislabeling in products, publications, and some publicly available DNA sequences, is discussed in the present work. The last findings in health promoting properties of L. paracasei and the bioactive compounds are described and compared to strains isolated from kefir, providing a special focus on exopolysaccharides as effector molecules. The knowledge of the state of the art of Lacticaseibacillus paracasei from kefir and water kefir can help to understand the contribution of these microorganisms to the health benefits of artisanal beverages as well as to discover new probiotic strains for applications in food industry.
Unified Scale Theorem: A Mathematical Formulation of Scale in the Frame of Earth Observation Image Classification
Authors: Karydas Christos G.
Published: September 2021

In this research, the geographic, observational, functional, and cartographic scale is unified into a single mathematical formulation for the purposes of earth observation image classification. Fractal analysis is used to define functional scales, which then are linked to the other concepts of scale using common equations and conditions. The proposed formulation is called Unified Scale Theorem (UST), and was assessed with Sentinel-2 image covering a variety of land uses from the broad area of Thessaloniki, Greece. Provided as an interactive excel spreadsheet, UST promotes objectivity, rapidity, and accuracy, thus facilitating optimal scale selection for image classification purposes.
Dynamics of Fractional-Order Digital Manufacturing Supply Chain System and Its Control and Synchronization
Authors: He Yingjin , Zheng Song , Yuan Liguo
Published: September 2021

Digital manufacturing is widely used in the production of automobiles and aircrafts, and plays a profound role in the whole supply chain. Due to the long memory property of demand, production, and stocks, a fractional-order digital manufacturing supply chain system can describe their dynamics more precisely. In addition, their control and synchronization may have potential applications in the management of real-word supply chain systems to control uncertainties that occur within it. In this paper, a fractional-order digital manufacturing supply chain system is proposed and solved by the Adomian decomposition method (ADM). Dynamical characteristics of this system are studied by using a phase portrait, bifurcation diagram, and a maximum Lyapunov exponent diagram. The complexity of the system is also investigated by means of SE complexity and C0 complexity. It is shown that the complexity results are consistent with the bifurcation diagrams, indicating that the complexity can reflect the dynamical properties of the system. Meanwhile, the importance of the fractional-order derivative in the modeling of the system is shown. Moreover, to further investigate the dynamics of the fractional-order supply chain system, we design the feedback controllers to control the chaotic supply chain system and synchronize two supply chain systems, respectively. Numerical simulations illustrate the effectiveness and applicability of the proposed methods.
Formalizing Opponent Modeling with the Rock, Paper, Scissors Game
Authors: Brockbank Erik , Vul Edward
Published: September 2021

In simple dyadic games such as rock, paper, scissors (RPS), people exhibit peculiar sequential dependencies across repeated interactions with a stable opponent. These regularities seem to arise from a mutually adversarial process of trying to outwit their opponent. What underlies this process, and what are its limits? Here, we offer a novel framework for formally describing and quantifying human adversarial reasoning in the rock, paper, scissors game. We first show that this framework enables a precise characterization of the complexity of patterned behaviors that people exhibit themselves, and appear to exploit in others. This combination allows for a quantitative understanding of human opponent modeling abilities. We apply these tools to an experiment in which people played 300 rounds of RPS in stable dyads. We find that although people exhibit very complex move dependencies, they cannot exploit these dependencies in their opponents, indicating a fundamental limitation in people’s capacity for adversarial reasoning. Taken together, the results presented here show how the rock, paper, scissors game allows for precise formalization of human adaptive reasoning abilities.
Ultrasound-Guided Percutaneous Drainage of Abdominal Collections—An Analysis over 5 Years
Authors: Stan-Ilie Madalina , Plotogea Oana-Mihaela , Rinja Ecaterina , Sandru Vasile , Butuc Andreea , Gheorghe Gina , Diaconu Camelia Cristina , Macovei Oprescu Anca , Popa Bogdan , Scafa-Udriste Alexandru , Constantinescu Gabriel
Published: September 2021

Introduction. Ultrasound-guided (US-guided) percutaneous drainage (PD) of abdominal collections represents the first-choice alternative to surgical intervention. The aim of our study was to assess the efficacy and safety of PD of visceral and non-visceral abdominal collections by reviewing our personal experience over a period of 5 years. Material and methods. The present study included 66 patients who underwent PD under ultrasound guidance. We analyzed clinical variables (collection size, catheter diameter, collection type, microbiological analysis, antibiotic regimens) along with the outcomes of the procedure. Results. Visceral collections were predominant, encompassing 38 hepatic abscesses and 1 splenic hematoma. Microbiological analysis showed that the majority (54%) were monomicrobial. The most encountered pathogens were Klebsiella pneumoniae and Escherichia coli. Technical success was achieved in all cases and clinical success was observed in 84.6% of the cases. No immediate procedural complications were detected. There were 6 patients who needed reinterventions, either by catheter replacement or by surgical treatment. The mortality rate was 4.5%, due to patients’ poor overall status and oncological comorbidities. Conclusions. Percutaneous drainage under ultrasound guidance is a safe and effective procedure in the management of abdominal collections.
Silica Xerogel Doped with Iron(III) as Sensor Material for Salicylhydroxamic Acid Determination in Urine
Authors: Morosanova Maria A. , Kovalev Vitaliy A. , Morosanova Elena I.
Published: September 2021

Salicylhydroxamic acid (SHA) is used as antimicrobic medicine and its concentration has to be monitored in urine. For the first time, silica xerogels doped with iron(III) have been proposed as sensor materials for SHA determination in biological samples. Three xerogels with iron(III) content in the range of 0.04–1.74% wt have been synthesized. BET surface area of these xerogels has varied in the range of 696–529 m2/g and total pore volume has varied in the range of 0.92–0.23 cm3/g. Complex formation between immobilized iron(III) and salicylhydroxamic acid has been investigated with solid phase spectrophotometry and IR spectroscopy. Orange-brown iron(III)-SHA complex with 1:1 stoichiometry is formed at pH 1–4 with half-reaction time of 17 min. Silica xerogel doped with 0.33% wt iron(III)) has been used as sensor material for SHA solid phase spectrophotometric determination (LOD 1.4 mg/L (n = 3), analytical range 4–230 mg/L). Proposed sensor material has been applied for SHA determination in biological samples of synthetic and human urine. The proposed procedure is characterized by a good level of accuracy (recovery values 97–120%) and precision (RSD values 4–9%) and can be recommended for pharmacokinetic–pharmacodynamic studies of hydroxamic acid-based medications.
Robust Silica-Bacterial Cellulose Composite Aerogel Fibers for Thermal Insulation Textile
Authors: Sai Huazheng , Wang Meijuan , Miao Changqing , Song Qiqi , Wang Yutong , Fu Rui , Wang Yaxiong , Ma Litong , Hao Yan
Published: September 2021

Aerogels are nanoporous materials with excellent properties, especially super thermal insulation. However, owing to their serious high brittleness, the macroscopic forms of aerogels are not sufficiently rich for the application in some fields, such as thermal insulation clothing fabric. Recently, freeze spinning and wet spinning have been attempted for the synthesis of aerogel fibers. In this study, robust fibrous silica-bacterial cellulose (BC) composite aerogels with high performance were synthesized in a novel way. Silica sol was diffused into a fiber-like matrix, which was obtained by cutting the BC hydrogel and followed by secondary shaping to form a composite wet gel fiber with a nanoscale interpenetrating network structure. The tensile strength of the resulting aerogel fibers reached up to 5.4 MPa because the quantity of BC nanofibers in the unit volume of the matrix was improved significantly by the secondary shaping process. In addition, the composite aerogel fibers had a high specific area (up to 606.9 m2/g), low density (less than 0.164 g/cm3), and outstanding hydrophobicity. Most notably, they exhibited excellent thermal insulation performance in high-temperature (210 °C) or low-temperature (−72 °C) environments. Moreover, the thermal stability of CAFs (decomposition temperature was about 330 °C) was higher than that of natural polymer fiber. A novel method was proposed herein to prepare aerogel fibers with excellent performance to meet the requirements of wearable applications.
Comparative Inhibition Study by Nanomaterial, Plant Extract and Chemical Microcide on the Screaming Mummy in Egyptian Museum Store
Authors: Ismael Sahar , Omar Ali , Maher Manal
Published: September 2021

Mummies in museums are exposed to different deterioration factors like microorganisms, especially unwrapped mummies, such as the screaming mummy. This screaming mummy in the store of the Egyptian museum is suffering from stains due to microbial infection. There are three trends of materials to inhibit microbial growth: nano materials, plant extraction and chemical materials. This research compares three materials representing the three trends such as nano zinc oxide (ZnO-NPs), Ceratophyllum demersum and 4-chloro-m-cresol, respectively. Microorganisms, isolated from the degraded mummy, were identified with an optical microscope and ribosomal ribonucleic acid (rRNA) analysis to guarantee identification accuracy. Results indicated that the bacteria in the mummy are Bacillus jeotgali, Kocuria turfanensis, Microbacterium imperial, Micrococcus luteus and Bacillus megaterium. Fungi are Monascus pallens and Rhizopus oryzae. The results of minimum inhibitory concentration (MIC) illustrated that the best concentrations for the bio treatment of isolated microorganisms is plant extract (Ceratophyllum demersum) at 600 ppm/100 mL, followed by 4-chloro-m-cresol at 600 ppm/100 mL and finally nano zinc oxide at 700 ppm/100 mL.
Overweight and Obesity in Patients with Congenital Heart Disease: A Systematic Review
Authors: Willinger Laura , Brudy Leon , Meyer Michael , Oberhoffer-Fritz Renate , Ewert Peter , Müller Jan
Published: September 2021

Background: Overweight and obesity have become a major public health concern in recent decades, particularly in patients with chronic health conditions like congenital heart disease (CHD). This systematic review elaborates on the prevalence and the longitudinal development of overweight and obesity in children and adults with CHD. Methods: A systematic literature search was conducted in PubMed, Cochrane, and Scopus from January 2010 to December 2020 on overweight and obesity prevalence in children and adults with CHD. Results: Of 30 included studies, 15 studies evaluated 5680 pediatric patients with CHD, 9 studies evaluated 6657 adults with CHD (ACHD) and 6 studies examined 9273 both pediatric patients and ACHD. Fifteen studies received the quality rating “good”, nine studies “fair”, and six studies “poor”. In children with CHD, overweight prevalence was between 9.5–31.5%, and obesity prevalence was between 9.5–26%; in ACHD, overweight prevalence was between 22–53%, and obesity was between 7–26%. The prevalence of overweight and obesity was thereby similar to the general population. Overweight and obesity have been shown to increase with age. Conclusion: The prevalence of overweight and obesity in children and adults with CHD is similar to the general population, demonstrating that the growing obesity pandemic is also affecting the CHD population.
Early or Delayed Onset of Food Intake in Time-Restricted Eating: Associations with Markers of Obesity in a Secondary Analysis of Two Pilot Studies
Authors: Kesztyüs Dorothea , Cermak Petra , Kesztyüs Tibor , Barzel Anne
Published: September 2021

Time-restricted eating (TRE) has rapidly gained interest in the public and the scientific community. One presumed mechanism of action is the adaptation of the eating–fasting rhythm to the evolutionary circadian rhythm of the metabolism. Study results regarding the suggestion that earlier beginning of food intake leads to better outcomes are heterogeneous. We conducted a secondary analysis of pooled data from two pilot studies on TRE to examine an association between the timing of onset of food intake with obesity-related outcomes. Participants (n = 99, 83 females aged 49.9 ± 10.8 years) were asked to restrict their daily eating to 8–9 h for three months. Tertiles of the onset of food intake were assessed for changes in anthropometry, blood lipid levels, and health-related quality of life. We detected no significant differences in outcomes between early (before 9:47), medium (9:47–10:50), and late onset (after 10:50) of food intake. However, the duration of the eating period was longest in the group with the earliest (8.6 ± 1.0 h) and shortest in the group with the latest onset (7.5 ± 0.8 h). Subsequently, fasting duration was longest in the last group (16.5 h). This may have compromised the results. More research is needed in this area to address this question.
Performances of Limited Area Models for the WORKLIMATE Heat–Health Warning System to Protect Worker’s Health and Productivity in Italy
Authors: Grifoni Daniele , Messeri Alessandro , Crisci Alfonso , Bonafede Michela , Pasi Francesco , Gozzini Bernardo , Orlandini Simone , Marinaccio Alessandro , Mari Riccardo , Morabito Marco ,   on behalf of the WORKLIMATE Collaborative Group
Published: September 2021

Outdoor workers are particularly exposed to climate conditions, and in particular, the increase of environmental temperature directly affects their health and productivity. For these reasons, in recent years, heat-health warning systems have been developed for workers generally using heat stress indicators obtained by the combination of meteorological parameters to describe the thermal stress induced by the outdoor environment on the human body. There are several studies on the verification of the parameters predicted by meteorological models, but very few relating to the validation of heat stress indicators. This study aims to verify the performance of two limited area models, with different spatial resolution, potentially applicable in the occupational heat health warning system developed within the WORKLIMATE project for the Italian territory. A comparison between the Wet Bulb Globe Temperature predicted by the models and that obtained by data from 28 weather stations was carried out over about three summer seasons in different daily time slots, using the most common skill of performance. The two meteorological models were overall comparable for much of the Italian explored territory, while major limits have emerged in areas with complex topography. This study demonstrated the applicability of limited area models in occupational heat health warning systems.
Novel Insights into Epigenetic Regulation of IL6 Pathway: In Silico Perspective on Inflammation and Cancer Relationship
Authors: Candido Saverio , Tomasello Barbara Maria Rita , Lavoro Alessandro , Falzone Luca , Gattuso Giuseppe , Libra Massimo
Published: September 2021

IL-6 pathway is abnormally hyperactivated in several cancers triggering tumor cell growth and immune system inhibition. Along with genomic mutation, the IL6 pathway gene expression can be affected by DNA methylation, microRNAs, and post-translational modifications. Computational analysis was performed on the Cancer Genome Atlas (TCGA) datasets to explore the role of IL6, IL6R, IL6ST, and IL6R transmembrane isoform expression and their epigenetic regulation in different cancer types. IL6 was significantly modulated in 70% of tumor types, revealing either up- or down-regulation in an approximately equal number of tumors. Furthermore, IL6R and IL6ST were downregulated in more than 10 tumors. Interestingly, the correlation analysis demonstrated that only the IL6R expression was negatively affected by the DNA methylation within the promoter region in most tumors. Meanwhile, only the IL6ST expression was extensively modulated by miRNAs including miR-182-5p, which also directly targeted all three genes. In addition, IL6 upregulated miR-181a-3p, mirR-214-3p, miR-18a-5p, and miR-938, which in turn inhibited the expression of IL6 receptors. Finally, the patients’ survival rate was significantly affected by analyzed targets in some tumors. Our results suggest the relevance of epigenetic regulation of IL6 signaling and pave the way for further studies to validate these findings and to assess the prognostic and therapeutic predictive value of these epigenetic markers on the clinical outcome and survival of cancer patients.