bluuebunny/crossref_metadata_2025 huggingface dataset:
Ventilation Rates Estimated from Tracers in the Presence of Mixing
Authors: Hall Timothy M. , Haine Thomas W. N. , Waugh Darryn W. , Holzer Mark , Terenzi Francesca , LeBel Deborah A.
Published: December 2007

The intimate relationship among ventilation, transit-time distributions, and transient tracer budgets is analyzed. To characterize the advective–diffusive transport from the mixed layer to the interior ocean in terms of flux we employ a cumulative ventilation-rate distribution, Φ(τ), defined as the one-way mass flux of water that resides at least time τ in the interior before returning. A one-way (or gross) flux contrasts with the net advective flux, often called the subduction rate, which does not accommodate the effects of mixing, and it contrasts with the formation rate, which depends only on the net effects of advection and diffusive mixing. As τ decreases Φ(τ) increases, encompassing progressively more one-way flux. In general, Φ is a rapidly varying function of τ (it diverges at small τ), and there is no single residence time at which Φ can be evaluated to fully summarize the advective–diffusive flux. To reconcile discrepancies between estimates of formation rates in a recent GCM study, Φ(τ) is used. Then chlorofluorocarbon data are used to bound Φ(τ) for Subtropical Mode Water and Labrador Sea Water in the North Atlantic Ocean. The authors show that the neglect of diffusive mixing leads to spurious behavior, such as apparent time dependence in the formation, even when transport is steady.
Impact of Channel Geometry and Rotation on the Trapping of Internal Tides
Authors: Drijfhout Sybren , Maas Leo R. M.
Published: December 2007

The generation and propagation of internal tides has been studied with an isopycnic three-dimensional ocean model. The response of a uniformly stratified sea in a channel, which is forced by a barotropic tide on its open boundary, is considered. The tide progresses into the channel and forces internal tides over a continental slope at the other end. The channel has a length of 1200 km and a width of 191.25 km. The bottom profile has been varied. In a series of four experiments it is shown how the cross-channel geometry affects the propagation and trapping of internal tides, and the penetration scale of wave energy, away from the continental slope, is discussed. In particular it is found that a cross-channel bottom slope constrains the penetration of the internal tidal energy. Most internal waves refract toward a cross-channel plane where they are trapped. The exception is formed by edge waves that carry part of the energy away from the continental slope. In the case of rotation near the continental slope, the Poincaré waves that arise in the absence of a cross-channel slope no longer bear the characteristics of the wave attractor predicted by 2D theory, but are almost completely arrested, while the right-bound Kelvin wave preserves the 2D attractor in the cross-channel plane, which is present in the nonrotating case. The reflected, barotropic right-bound Kelvin wave acts as a secondary internal wave generator along the cross-channel slope.
Evaluation of Interior Circulation in a High-Resolution Global Ocean Model. Part II: Southern Hemisphere Intermediate, Mode, and Thermocline Waters
Authors: Sen Gupta Alexander , England Matthew H.
Published: December 2007

A high-resolution, offline ocean general circulation model, incorporating a realistic parameterization of mixed layer convection, is used to diagnose pathways and time scales of Southern Hemisphere intermediate, mode, and lower thermocline water ventilation. The use of such an offline methodology represents the only feasible way of simulating the long time scales required to validate the internal pathways of a high-resolution ocean model. Simulated and observed chlorofluorocarbon-11 (CFC-11) are in reasonably good agreement, demonstrating the model’s skill in representing realistic ventilation. Regional passive dye and age tracer experiments aid in the identification of pathways originating from different Southern Hemisphere locations. Northern Hemisphere penetration of intermediate, mode, and thermocline waters is most extensive and rapid into the North Atlantic Ocean because these waters are involved in closing the Atlantic meridional overturning cell. However, less than 8% of this ventilation is derived from subduction within the South Atlantic in the simulation. Instead, this water enters the Atlantic just to the south of South Africa, having originally subducted primarily in the east Indian Ocean, but also in the west Indian Ocean and the west Pacific region where a pathway advects water westward to the south of Australia. This pathway also plays a large part, together with water overturned in the east Indian Ocean, in ventilating the northern reaches of the Indian basin. Northward propagation in the Pacific Ocean is limited to the low latitudes of the Northern Hemisphere and is almost exclusively accomplished by water subducted in the South Pacific. A small contribution is made from the other basins from water that spreads northward, fed by a circumpolar pathway associated with the Antarctic Circumpolar Current that forms a conduit for intermediate and mode water exchange between all three basins. Intermediate water is injected into and branches off this pathway in all basins, but most vigorously in the southeastern Pacific.
Interannual Variations in Upper-Ocean Heat Content and Heat Transport Convergence in the Western North Atlantic
Authors: Dong Shenfu , Hautala Susan L. , Kelly Kathryn A.
Published: December 2007

Subsurface temperature data in the western North Atlantic Ocean are analyzed to study the variations in the heat content above a fixed isotherm and contributions from surface heat fluxes and oceanic processes. The study region is chosen based on the data density; its northern boundary shifts with the Gulf Stream position and its southern boundary shifts to contain constant volume. The temperature profiles are objectively mapped to a uniform grid (0.5° latitude and longitude, 10 m in depth, and 3 months in time). The interannual variations in upper-ocean heat content show good agreement with the changes in the sea surface height from the Ocean Topography Experiment (TOPEX)/Poseidon altimeter; both indicate positive anomalies in 1994 and 1998–99 and negative anomalies in 1996–97. The interannual variations in surface heat fluxes cannot explain the changes in upper-ocean heat storage rate. On the contrary, a positive anomaly in heat released to the atmosphere corresponds to a positive upper-ocean heat content anomaly. The oceanic heat transport, mainly owing to the geostrophic advection, controls the interannual variations in heat storage rate, which suggests that geostrophic advection plays an important role in the air–sea heat exchange. The 18°C isotherm depth and layer thickness also show good correspondence to the upper-ocean heat content; a deep and thin 18°C layer corresponds to a positive heat content anomaly. The oceanic transport in each isotherm layer shows an annual cycle, converging heat in winter, and diverging in summer in a warm layer; it also shows interannual variations with the largest heat convergence occurring in even warmer layers during the period of large ocean-to-atmosphere flux.
The Role of Wind Stress Curl in Jet Separation at a Cape
Authors: Castelao Renato M. , Barth John A.
Published: December 2007

A high-resolution numerical model is used to study the importance of spatial variability in the wind forcing to the separation of a coastal upwelling jet at a cape. An idealized topography and wind field based on observations from the Cape Blanco (Oregon) region are used. Several simulations are investigated, with both the intensity and the spatial structure of the wind forcing varied to isolate the importance of the observed intensification in the wind stress and wind stress curl magnitudes to the separation process. A simulation using a straight coast confirms that the presence of the cape is crucial for separation. Wind stress intensification by itself, with zero curl, does not aid separation. The wind stress curl intensification south of the cape, on the other hand, is important for controlling details of the process. Because the positive wind stress curl drives upwelling, isotherms in the offshore region tilt upward, creating a pressure gradient that sustains an intensification of the southward velocities via the thermal wind balance. This aids jet separation via continuity and by creating potential vorticity contours that track far offshore of the cape. The timing of the separation is dependent on the intensity of the wind stress curl (stronger curl leads to earlier separation), while how far offshore the jet is deflected depends on the offshore extent of the region of positive curl close to the coast (increasing the extent increases the deflection).
The Coastal Bottom Boundary Layer: A Note on the Model of Chapman and Lentz
Authors: Pedlosky Joseph
Published: December 2007

The bottom boundary layer of a stratified flow on a coastal continental shelf is examined using the model of Chapman and Lentz. The flow is driven by a surface stress, uniform in the alongshore coordinate, in a downwelling-favorable direction. The stress diminishes in the offshore direction and produces an Ekman pumping, as well as an onshore Ekman flux. The model yields an interior flow, sandwiched between an upper Ekman layer and a bottom boundary layer. The interior has a horizontal density gradient produced by a balance between horizontal diffusion of density and vertical advection of a background vertical density gradient. The interior flow is vertically sheared and in thermal wind balance. Whereas the original model of Chapman and Lentz considered an alongshore flow that is freely evolving, the present note focuses on the equilibrium structure of a flow driven by stress and discusses the vertical and lateral structure of the flow and, in particular, the boundary layer thickness. The vertical diffusivity of density in the bottom boundary layer is considered so strong, locally, as to render the bottom boundary layer’s density a function of only offshore position. Boundary layer budgets of mass, momentum, and buoyancy determine the barotropic component of the interior flow as well as the boundary layer thickness, which is a function of the offshore coordinate. The alongshore flow has enhanced vertical shear in the boundary layer that reduces the alongshore flow in the boundary layer; however, the velocity at the bottom is generally not zero but produces a stress that locally balances the applied surface stress. The offshore transport in the bottom boundary layer therefore balances the onshore surface Ekman flux. The model predicts the thickness of the bottom boundary layer, which is a complicated function of several parameters, including the strength of the forcing stress, the vertical and horizontal diffusion coefficients in the interior, and the horizontal diffusion in the boundary layer. The model yields a boundary layer over only a finite portion of the bottom slope if the interior diffusion coefficients are too large; otherwise, the layer extends over the full lateral extent of the domain.
Wave-Follower Field Measurements of the Wind-Input Spectral Function. Part III: Parameterization of the Wind-Input Enhancement due to Wave Breaking
Authors: Babanin Alexander V. , Banner Michael L. , Young Ian R. , Donelan Mark A.
Published: December 2007

This is the third in a series of papers describing wave-follower observations of the aerodynamic coupling between wind and waves on a large shallow lake during the Australian Shallow Water Experiment (AUSWEX). It focuses on the long-standing problem of the aerodynamic consequences of wave breaking on the wind–wave coupling. Direct field measurements are reported of the influence of wave breaking on the wave-induced pressure in the airflow over water waves, and hence the energy flux to the waves. The level of forcing, measured by the ratio of wind speed to the speed of the dominant (spectral peak) waves, covered the range of 3–7. The propagation speeds of the dominant waves were limited by the water depth and the waves were correspondingly steep. These measurements allowed an assessment of the magnitude of any breaking-induced enhancement operative for these field conditions and provided a basis for parameterizing the effect. Overall, appreciable levels of wave breaking occurred for the strong wind forcing conditions that prevailed during the observational period. Associated with these breaking wave events, a significant phase shift is observed in the local wave-coherent surface pressure. This produced an enhanced wave-coherent energy flux from the wind to the waves with a mean value of 2 times the corresponding energy flux to the nonbreaking waves. It is proposed that the breaking-induced enhancement of the wind input to the waves can be parameterized by the sum of the nonbreaking input and the contribution due to the breaking probability.
Cecil Foster's Sleep on, Beloved
Authors: Thomas H. Nigel
Published: December 2007

This essay focuses on the pathology and alienation of Blacks in Cecil Foster's fictional Toronto. Space permits no more than a cursory analysis of why Blacks immigrate to Canada in large numbers. The author complements his analysis of Sleep on, Beloved with information from A Place Called Heaven and uses the latter to provide a context for the former. The exigencies of form—the novel and the essay—notwithstanding, the author does not feel that this approach distorts Foster's intentions inasmuch as Foster told the author that the single goal of his writing is to faithfully record and interpret reality to the best of his ability. It is worth noting too that in A Place Called Heaven, Foster's sense of life, which forms the psycho-epistemological foundation for his works, is expressed in minute detail. Underlying the analysis is the notion that place, unless the artist is inept, is always a major trope.
Lack of a Pharmacokinetic Effect of Raltegravir on Midazolam: In Vitro/In Vivo Correlation
Authors: Iwamoto Marian , Kassahun Kelem , Troyer Matthew D. , Hanley William D. , Lu Ping , Rhoton Alisha , Petry Amelia S. , Ghosh Kalyan , Mangin Eric , DeNoia Emanuel P. , Wenning Larissa A. , Stone Julie A. , Gottesdiener Keith M. , Wagner John A.
Published: December 2007

Raltegravir is a novel HIV‐1 integrase inhibitor with potent in vitro activity (95% inhibitory concentration = 33 nM in 50% human serum). In vitro characterization of raltegravir inhibition potential was assessed against a panel of cytochrome P450 (CYP) enzymes. An open‐label, 2‐period study was conducted to assess the effect of raltegravir on the pharmacokinetics of midazolam, a sensitive CYP 3A4 probe substrate: period 1, 2.0 mg of midazolam; period 2, 400 mg of raltegravir every 12 hours for 14 days with 2.0 mg of midazolam on day 14. There was no meaningful in vitro effect of raltegravir on inhibition of a panel of CYP enzymes and induction of CYP 3A4. In the presence of raltegravir, midazolam area under the curve extrapolated to infinity (AUC0‐infin) and maximum plasma concentration (Cmax) geometric mean ratios were similar (geometric mean ratios and 90% confidence intervals: 0.92 [0.82, 1.03] (P = .208) and 1.03 [0.87, 1.22] (P = .751), respectively). No substantial differences were observed in Tmax (P = .750) or apparent half‐life (P = .533) of midazolam. Plasma levels of midazolam were not substantially affected by raltegravir, which implies that raltegravir is not a clinically important inducer or inhibitor of CYP 3A4 and that raltegravir would not be expected to affect the pharmacokinetics of other drugs metabolized by CYP 3A4 to a clinically meaningful extent.
Adult age differences in reading and rereading processes associated with problem solving
Authors: Soederberg Miller Lisa M. , Gagne Danielle D.
Published: December 2007

We investigated age differences in reading and rereading processes associated with problem solving and explored the extent to which prior information affects rereading processes. Participants' reading times were recorded as they read short mysteries, twice, at their own pace on a computer, with the goal of providing the solution to the mystery. We varied the amount of information provided prior to rereading the mysteries such that participants received: no new information, a hint (partial information), or the full solution. Reading times for trial 1 and for all three rereading conditions were decomposed to determine resource allocation to specific reading processes including conceptual integration, attention to critical regions, and instantiation of new characters in the narrative. We found that younger and older adults attended to critical regions of the problem similarly on trial 1 as well as when rereading with no information or a hint. Age differences were found, however, in the effects of rereading with prior information on conceptual integration. For older relative to younger adults, a hint was more effortful (as reflected in conceptual processing time) and was not as helpful (as reflected in problem-solving accuracy scores). However, older adults who increased time to conceptual integration when applying a hint had higher performance on the second trial, suggesting increased integration is an effective strategy when utilizing new information.
The myth of the alpha male: A new look at dominance-related beliefs and behaviors among adolescent males and females
Authors: Hawley Patricia H. , Little Todd D. , Card Noel A.
Published: December 2007

Evolutionary and biological approaches tend to suggest that social dominance is predominately an aspect of male social organization. Furthermore, when females behave non-normatively, they are less positively evaluated than males engaging in the same behavior. Alternate, less familiar models of females and dominance/aggression underlie the present study which proposes that dominant males and females are more similar in both behavioral profile and social reception than commonly believed. Participants ( N = 1723; grades 5—10) self-rated their aggression, social motivations, and strategies and beliefs associated with interpersonal influence. Peer ratings of strategies of influence, aggression, and the degree to which peers were liked and disliked were also obtained. Results demonstrated that socially dominant males and females balance prosocial and coercive strategies and win positive peer regard, their aggressiveness notwithstanding. These findings highlight competitiveness in females and provide insights into the paradoxical relationship between positive peer regard and aggression (the peer regard—aggression paradox).
Staying in the (Curricular) Lines
Authors: Dyson Anne Haas
Published: December 2007

Young children are growing up in a time when literacy practices and textual productions are in flux. Yet literacy curricula, particularly for those deemed “at risk,” are tightly focused on the written language “basics.” What are the potential consequences? In this article, the author considers this question, drawing on an ethnographic study of child writing in an urban school site. Using a sociocultural and dialogic frame, she examines first graders' interpretations and negotiations of official writing practices, detailing how these (a) shaped their written language use, including use of time and space, multimodal tools, and expected voices and modeled ideologies and (b) pushed to the sidelines or left in the unofficial child world aspects of their knowledge and know-how, including a breadth of communicative practices and a diversity of graphological symbols. The author concludes with reflections on instructional links among official writing practices, children's literacy experiences, and the “basics” in contemporary times.
Understanding and Reducing the Knowledge Effect: Implications for Writers
Authors: Hayes John R. , Bajzek Diana
Published: December 2007

To be effective, writers must understand what knowledge they share with the audience and what they do not. Achieving this understanding is made difficult by the knowledge effect—a tendency of individuals to assume that their own knowledge is shared by others. Understanding the knowledge effect and methods for reducing it is potentially useful for understanding and teaching writing. In Study 1, we explored the impact of an individual's knowledge of technical terms on that person's ability to estimate other people's understanding of those terms. We assessed how individuals' familiarity with technical terms influenced their predictions that college freshmen and college graduates would understand those terms. Results indicate that familiarity with the meaning of technical terms leads to substantial overestimation of others' knowledge. In Study 2, we evaluated an online tutor designed to improve writers' predictions of other's word knowledge by providing them with feedback on the accuracy of their judgments.
The Importance of the State: Political Dimensions of a Nonprofit Network in Oaxaca, Mexico
Authors: Neal Rachael
Published: December 2007

This article examines a network of nonprofit nongovernmental organizations (NGOs), government agencies, and other social and political actors in Oaxaca, Mexico. Analyses indicate that NGOs are more connected to government authorities than to any type of nonstate organization. Results also suggest that NGOs located in municipalities where support was the highest and lowest in the 1994 to 2001 elections for the long-ruling Partido Revolucionario Institucional were best connected to the government. The size of Indigenous populations located around the NGOs also appears to have had an impact on NGO connectedness to the government. These results offer support for social origins theory, which acknowledges the impact of social—political environments on the development of NGO sectors.
Wrestling With Proteus
Authors: Amidon Stevens , Blythe Stuart
Published: December 2007

Because communication specialists often lack the power and prestige of other knowledge workers, such as engineers and product designers, managers who direct the work of communication specialists face unique challenges. This study, based on interviews with 11 communication managers, found that their agency and identity were determined both by the structure of the organizations in which they worked and by their use of genres, technologies, and regulatory techniques. With their work undergoing transition because of globalization, outsourcing, and rapid technological change, the stories that these managers tell demonstrate the importance of studying management as it specifically applies to communication specialists.
Mode, Medium, and Genre
Authors: Graham S. Scott , Whalen Brandon
Published: December 2007

Recently, scholars of new media have been exploring the relationships between genre theory and new media. While these scholars have provided a great deal of insight into the nature of e-genres and how they function in professional contexts, few address the relationship between genre and new-media theories from a designer's perspective. This article presents the results of an ethnographic-style case study exploring the practice of a professional new-media designer. These results (a) confirm the role of dynamic rhetorical situations and hybridity during the new-media design process; (b) suggest that current genre and new-media theories underestimate the complexity of the relationships between mode, medium, genre, and rhetorical exigencies; and (c) indicate that a previously unrecognized form of hybridity exists in contemporary e-genres.
Eating Behavior in Persons With Moderate to Late-stage Dementia: Assessment and Interventions
Authors: Amella Elaine J. , Grant Alicia P. , Mulloy Cathy
Published: December 2007

Persons with various forms of dementia suffer from a progressive disease in which memory and the ability to function independently are lost. During moderate to late-stage dementia, individuals experience increased difficulty with eating and require more feeding assistance. Clinicians working with this population must acknowledge the multifactorial aspects of eating behavioral issues, use a team approach, and make careful assessments using appropriate instruments. Interventions should include attention to cognitive impairment, nutritional intake, training of caregivers, modification of the environment, and the quality of the interaction. Planning for care should include promoting the highest quality of life for these individuals and their caregivers. J Am Psychiatr Nurses Assoc, 2008; 13(6), 360-367.
Weight-Related Concerns and Behaviors in Children and Adolescents With Type 1 Diabetes
Authors: Howe Carol J. , Jawad Abbas F. , Kelly Sarah D. , Lipman Terri H.
Published: December 2007

Children and adolescents with type 1 diabetes are at risk for disordered eating and unhealthy weight-control practices. This study describes (a) participants' weight perception and weight satisfaction, (b) participants' scores on the Diabetes Eating Problem Survey (DEPS), (c) prevalence of weight-control behaviors, and (d) association of DEPS and weight-control behaviors with race, gender, age, body mass index (BMI), hemoglobin A1c (HbA1c), weight satisfaction, and weight perception. TheAHEAD survey was completed by 295 participants to determine weight satisfaction, weight perception, and weight-control behaviors. Height, weight, and HbA1c were obtained from clinic charts. Older females with higher BMI and elevated HbA1c used significantly more weight-control behaviors. Weight dissatisfaction and heavy weight perception were associated with significantly more unhealthy weight-control practices. Prevention programs should be directed toward the preteen female. Older female teens presenting with higher BMI, elevated HbA1c, weight dissatisfaction, and heavy weight perception should be formally assessed for unhealthy weight-control behaviors. J Am Psychiatr Nurses Assoc, 2008; 13(6), 376-385. DOI: 10.1177/1078390307310154
Implementation of Possession Laws and the Social Ecology of Tobacco Control
Authors: Livingood William C. , Woodhouse Lynn D. , Wludyka Peter
Published: December 2007

The objective of this evaluation research was to assess the impact of programs intended to support the enforcement component of a comprehensive youth tobacco control. The research method was a survey of a randomly stratified cluster sample of law enforcement officers. Results of the evaluation showed that the enforcement behaviors of officers were increased through the state programs to support tobacco enforcement activities. The study showed that support for implementing a policy is important to achieve the objectives of a policy. The results of a study of the enforcement component of a Florida tobacco control program are reported and discussed within the ecological context of previously reported enforcement-linked decreases in youth tobacco use and funding and defunding of the Florida Tobacco Control Program.
Identifying Belief-Based Targets for the Promotion of Leisure-Time Walking
Authors: Rhodes Ryan E. , Blanchard Chris M. , Courneya Kerry S. , Plotnikoff Ronald C.
Published: December 2007

Walking is the most common type of physical activity (PA) and the likely target of efforts to increase PA. No studies, however, have identified the belief-level correlates for walking using the theory of planned behavior. This study elicits salient beliefs about walking and evaluates beliefs that may be most important for walking-promotion campaigns. A random sample of 55 Canadian adults participated in the elicitation study, and the validation study includes 358 participants who completed measures of demographics, beliefs from the elicitation study, and self-reported walking behavior. Belief—behavior relationships show that almost all behavioral expectancies are correlates of intention and behavior, but normative and control expectancies are more select correlates. But when belief—behavior relations are evaluated by participants meeting PA recommendations, only beliefs about feeling good, appearance, stress relief, and time are significant. Public health efforts to promote walking should focus on making time for regular walking and the affective advantages that walking can provide.
A Cervical Cancer Community-Based Participatory Research Project in a Native American Community
Authors: Christopher Suzanne , Gidley Allison L. , Letiecq Bethany , Smith Adina , McCormick Alma Knows His Gun
Published: December 2007

The Messengers for Health on the Apsáalooke Reservation project uses a community-based participatory research (CBPR) approach and lay health advisors (LHAs) to generate knowledge and awareness about cervical cancer prevention among community members in a culturally competent manner. Northern Plains Native Americans, of whom Apsáalooke women are a part, continue to be disproportionately affected by cervical cancer. This article examines quantitative and qualitative changes that occurred in the community since the inception of the Messengers for Health program. Paired sample t tests are used to evaluate the one-group pretest and posttest interviews of 83 Apsáalooke women in knowledge, comfort, and cancer awareness levels. Results reveal cervical cancer knowledge gains, gains in participants' comfort discussing cancer issues, and gains in awareness of cervical cancer and the Messengers program. Field notes, meeting minutes, and community perceptions are used to qualitatively evaluate the effectiveness of the Messengers program. Practice implications are discussed.
(Re)considering Voice
Authors: Gray Jennie
Published: December 2007

This article considers some of complexities around a feminist imperative of `voice'. It is a reflective dialogue that follows the possibilities and problematics encountered in a social inquiry aimed at creating space for the women who participated to `give voice' as a collective. Our explorations about the ways that these women, diagnosed with a psychiatric disorder, come to experience their everyday worlds as they do, involved identifying the social processes and practices shaping their lived actualities. Speaking together and back to the discourses of biomedicine that work to position these women in fixed and universal ways was also central to our researching. That the woman's voice goes largely unheard when spoken from `madness' made such work more urgent. As our navigations of speaking together shows, however, this was not as straightforward as we had envisaged: because the circumstances that produce experiences of oppression are rarely simple, social change efforts to ameliorate inequities will necessarily need to be nuanced. This polyphonic discussion honours the respective contributions to our knowledge-making endeavours, and tells of a project cast in feminism's `with'.
Untitled Moments
Authors: Phillips Catherine
Published: December 2007

In this article I argue that social work practice is continually informed by not just a corporeal or physical body, but also by an incorporeal body. Incorporeal knowledge. I am interested in how such a body invokes questions about how we theorize (in/through) practice. To examine these questions, I work with the postmodern theoretical stance that the separation of practice and theory in social work is not tenable (Fook, 1996; Fook, 2002; John, 1994; Rossiter, 2000). I narrate effects on my body of trauma work that have been framed by the incorporeal body.
Potentially oncogenic B-cell activation–induced smaller isoforms of FOXP1 are highly expressed in the activated B cell–like subtype of DLBCL
Authors: Brown Philip J. , Ashe Sally L. , Leich Ellen , Burek Christof , Barrans Sharon , Fenton James A. , Jack Andrew S. , Pulford Karen , Rosenwald Andreas , Banham Alison H.
Published: December 2007

The FOXP1 forkhead transcription factor is targeted by recurrent chromosome translocations in several subtypes of B-cell non-Hodgkin lymphomas, where high-level FOXP1 protein expression has been linked to a poor prognosis. Western blotting studies of diffuse large B-cell lymphoma (DLBCL) cell lines unexpectedly identified the atypical high-level expression of 2 smaller, 60 to 65 kDa, FOXP1 isoforms in all 5 of those with the activated B cell (ABC)–like DLBCL subtype and in a subgroup of primary DLBCL. The anti-FOXP1 (JC12) monoclonal antibody cannot distinguish FOXP1 isoforms by immunohistochemistry, a finding that may be clinically relevant as high-level expression of the full-length FOXP1 protein was observed in some germinal center–derived DLBCLs. ABC-like DLBCL-derived cell lines were observed to express 2 novel, alternatively spliced FOXP1 mRNA isoforms, encoding N-terminally truncated proteins. These transcripts and the smaller protein isoforms were induced as a consequence of normal B-cell activation, which thus represents an additional mechanism for up-regulating FOXP1 expression in lymphomas. The expression of potentially oncogenic smaller FOXP1 isoforms may resolve the previously contradictory findings that FOXP1 represents a favorable prognostic marker in breast cancer and an adverse risk factor in B-cell lymphomas.
On the analysis of glycomics mass spectrometry data via the regularized area under the ROC curve
Authors: Ye Jingjing , Liu Hao , Kirmiz Crystal , Lebrilla Carlito B , Rocke David M
Published: December 2007

Background

Novel molecular and statistical methods are in rising demand for disease diagnosis and prognosis with the help of recent advanced biotechnology. High-resolution mass spectrometry (MS) is one of those biotechnologies that are highly promising to improve health outcome. Previous literatures have identified some proteomics biomarkers that can distinguish healthy patients from cancer patients using MS data. In this paper, an MS study is demonstrated which uses glycomics to identify ovarian cancer. Glycomics is the study of glycans and glycoproteins. The glycans on the proteins may deviate between a cancer cell and a normal cell and may be visible in the blood. High-resolution MS has been applied to measure relative abundances of potential glycan biomarkers in human serum. Multiple potential glycan biomarkers are measured in MS spectra. With the objection of maximizing the empirical area under the ROC curve (AUC), an analysis method was considered which combines potential glycan biomarkers for the diagnosis of cancer.

Results

Maximizing the empirical AUC of glycomics MS data is a large-dimensional optimization problem. The technical difficulty is that the empirical AUC function is not continuous. Instead, it is in fact an empirical 0–1 loss function with a large number of linear predictors. An approach was investigated that regularizes the area under the ROC curve while replacing the 0–1 loss function with a smooth surrogate function. The constrained threshold gradient descent regularization algorithm was applied, where the regularization parameters were chosen by the cross-validation method, and the confidence intervals of the regression parameters were estimated by the bootstrap method. The method is called TGDR-AUC algorithm. The properties of the approach were studied through a numerical simulation study, which incorporates the positive values of mass spectrometry data with the correlations between measurements within person. The simulation proved asymptotic properties that estimated AUC approaches the true AUC. Finally, mass spectrometry data of serum glycan for ovarian cancer diagnosis was analyzed. The optimal combination based on TGDR-AUC algorithm yields plausible result and the detected biomarkers are confirmed based on biological evidence.

Conclusion

The TGDR-AUC algorithm relaxes the normality and independence assumptions from previous literatures. In addition to its flexibility and easy interpretability, the algorithm yields good performance in combining potential biomarkers and is computationally feasible. Thus, the approach of TGDR-AUC is a plausible algorithm to classify disease status on the basis of multiple biomarkers.
Unravelling the molecular control of calvarial suture fusion in children with craniosynostosis
Authors: Coussens Anna K , Wilkinson Christopher R , Hughes Ian P , Morris C Phillip , van Daal Angela , Anderson Peter J , Powell Barry C
Published: December 2007

Background

Craniosynostosis, the premature fusion of calvarial sutures, is a common craniofacial abnormality. Causative mutations in more than 10 genes have been identified, involving fibroblast growth factor, transforming growth factor beta, and Eph/ephrin signalling pathways. Mutations affect each human calvarial suture (coronal, sagittal, metopic, and lambdoid) differently, suggesting different gene expression patterns exist in each human suture. To better understand the molecular control of human suture morphogenesis we used microarray analysis to identify genes differentially expressed during suture fusion in children with craniosynostosis. Expression differences were also analysed between each unfused suture type, between sutures from syndromic and non-syndromic craniosynostosis patients, and between unfused sutures from individuals with and without craniosynostosis.

Results

We identified genes with increased expression in unfused sutures compared to fusing/fused sutures that may be pivotal to the maintenance of suture patency or in controlling early osteoblast differentiation (i.e.RBP4,GPC3,C1QTNF3,IL11RA,PTN,POSTN). In addition, we have identified genes with increased expression in fusing/fused suture tissue that we suggest could have a role in premature suture fusion (i.e.WIF1,ANXA3,CYFIP2). Proteins of two of these genes, glypican 3 and retinol binding protein 4, were investigated by immunohistochemistry and localised to the suture mesenchyme and osteogenic fronts of developing human calvaria, respectively, suggesting novel roles for these proteins in the maintenance of suture patency or in controlling early osteoblast differentiation. We show that there is limited difference in whole genome expression between sutures isolated from patients with syndromic and non-syndromic craniosynostosis and confirmed this by quantitative RT-PCR. Furthermore, distinct expression profiles for each unfused suture type were noted, with the metopic suture being most disparate. Finally, although calvarial bones are generally thought to grow without a cartilage precursor, we show histologically and by identification of cartilage-specific gene expression that cartilage may be involved in the morphogenesis of lambdoid and posterior sagittal sutures.

Conclusion

This study has provided further insight into the complex signalling network which controls human calvarial suture morphogenesis and craniosynostosis. Identified genes are candidates for targeted therapeutic development and to screen for craniosynostosis-causing mutations.
Imparting carrier status results detected by universal newborn screening for sickle cell and cystic fibrosis in England: a qualitative study of current practice and policy challenges
Authors: Parker Hilda , Qureshi Nadeem , Ulph Fiona , Kai Joe
Published: December 2007

Background

Universal newborn screening for early detection of children affected by sickle cell disorders and cystic fibrosis is currently being implemented across England. Parents of infants identified as carriers of these disorders must also be informed of their baby's result. However there is a lack of evidence for most effective practice internationally when doing so. This study describes current or proposed models for imparting this information in practice and explores associated challenges for policy.

Methods

Thematic analysis of semi-structured interviews with Child Health Coordinators from all English Health Regions.

Results

Diverse methods for imparting carrier results, both within and between regions, and within and between conditions, were being implemented or planned. Models ranged from result by letter to in-person communication during a home visit. Non-specialists were considered the best placed professionals to give results and a similar approach for both conditions was emphasised. While national guidance has influenced choice of models, other factors contributed such as existing service structures and lack of funding. Challenges included uncertainty about guidance specifying face to face notification; how best to balance allaying parental anxiety by using familiar non-specialist health professionals with concerns about practitioner competence; and extent of information parents should be given. Inadequate consideration of resource and service workload was seen as the main policy obstacle. Clarification of existing guidance; more specific protocols to ensure consistent countrywide practice; integration of the two programmes; and 'normalising' carrier status were suggested as improvements.

Conclusion

Differing models for communicating carrier results raise concerns about equity and clinical governance. However, this variation provides opportunity for evaluation. Timely and more detailed guidance on protocols with clarification of existing recommendations is needed.
Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats
Authors: Rodrigues Bruno , Figueroa Diego M , Mostarda Cristiano T , Heeren Marcelo V , Irigoyen Maria-Cláudia , De Angelis Kátia
Published: December 2007

Background

The aim of the present study was to investigate the relationship between speed during maximum exercise test (ET) and oxygen consumption (VO2) in control and STZ-diabetic rats, in order to provide a useful method to determine exercise capacity and prescription in researches involving STZ-diabetic rats.

Methods

Male Wistar rats were divided into two groups: control (CG, n = 10) and diabetic (DG, n = 8). The animals were submitted to ET on treadmill with simultaneous gas analysis through open respirometry system. ET and VO2 were assessed 60 days after diabetes induction (STZ, 50 mg/Kg).

Results

VO2 maximum was reduced in STZ-diabetic rats (72.5 ± 1 mL/Kg/min-1) compared to CG rats (81.1 ± 1 mL/Kg/min-1). There were positive correlations between ET speed and VO2 (r = 0.87 for CG and r = 0.8 for DG), as well as between ET speed and VO2 reserve (r = 0.77 for CG and r = 0.7 for DG). Positive correlations were also obtained between measured VO2 and VO2 predicted values (r = 0.81 for CG and r = 0.75 for DG) by linear regression equations to CG (VO2 = 1.54 * ET speed + 52.34) and DG (VO2 = 1.16 * ET speed + 51.99). Moreover, we observed that 60% of ET speed corresponded to 72 and 75% of VO2 reserve for CG and DG, respectively. The maximum ET speed was also correlated with VO2 maximum for both groups (CG: r = 0.7 and DG: r = 0.7).

Conclusion

These results suggest that: a) VO2 and VO2 reserve can be estimated using linear regression equations obtained from correlations with ET speed for each studied group; b) exercise training can be prescribed based on ET in control and diabetic-STZ rats; c) physical capacity can be determined by ET. Therefore, ET, which involves a relatively simple methodology and low cost, can be used as an indicator of cardio-respiratory capacity in future studies that investigate the physiological effect of acute or chronic exercise in control and STZ-diabetic male rats.
Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS
Authors: Sterjovski Jasminka , Churchill Melissa J , Ellett Anne , Gray Lachlan R , Roche Michael J , Dunfee Rebecca L , Purcell Damian FJ , Saksena Nitin , Wang Bin , Sonza Secondo , Wesselingh Steven L , Karlsson Ingrid , Fenyo Eva-Maria , Gabuzda Dana , Cunningham Anthony L , Gorry Paul R
Published: December 2007

Background

CCR5-restricted (R5) human immunodeficiency virus type 1 (HIV-1) variants cause CD4+ T-cell loss in the majority of individuals who progress to AIDS, but mechanisms underlying the pathogenicity of R5 strains are poorly understood. To better understand envelope glycoprotein (Env) determinants contributing to pathogenicity of R5 viruses, we characterized 37 full-length R5 Envs from cross-sectional and longitudinal R5 viruses isolated from blood of patients with asymptomatic infection or AIDS, referred to as pre-AIDS (PA) and AIDS (A) R5 Envs, respectively.

Results

Compared to PA-R5 Envs, A-R5 Envs had enhanced fusogenicity in quantitative cell-cell fusion assays, and reduced sensitivity to inhibition by the fusion inhibitor T-20. Sequence analysis identified the presence of Asn 362 (N362), a potential N-linked glycosylation site immediately N-terminal to CD4-binding site (CD4bs) residues in the C3 region of gp120, more frequently in A-R5 Envs than PA-R5 Envs. N362 was associated with enhanced fusogenicity, faster entry kinetics, and increased sensitivity of Env-pseudotyped reporter viruses to neutralization by the CD4bs-directed Env mAb IgG1b12. Mutagenesis studies showed N362 contributes to enhanced fusogenicity of most A-R5 Envs. Molecular models indicate N362 is located adjacent to the CD4 binding loop of gp120, and suggest N362 may enhance fusogenicity by promoting greater exposure of the CD4bs and/or stabilizing the CD4-bound Env structure.

Conclusion

Enhanced fusogenicity is a phenotype of the A-R5 Envs studied, which was associated with the presence of N362, enhanced HIV-1 entry kinetics and increased CD4bs exposure in gp120. N362 contributes to fusogenicity of R5 Envs in a strain dependent manner. Our studies suggest enhanced fusogenicity of A-R5 Envs may contribute to CD4+ T-cell loss in subjects who progress to AIDS whilst harbouring R5 HIV-1 variants. N362 may contribute to this effect in some individuals.
HTLV-1 HBZ cooperates with JunD to enhance transcription of the human telomerase reverse transcriptase gene (hTERT)
Authors: Kuhlmann Anne-Sophie , Villaudy Julien , Gazzolo Louis , Castellazzi Marc , Mesnard Jean-Michel , Duc Dodon Madeleine
Published: December 2007

Background

Activation of telomerase is a critical and late event in tumor progression. Thus, in patients with adult-T cell leukaemia (ATL), an HTLV-1 (Human T cell Leukaemia virus type 1)-associated disease, leukemic cells display a high telomerase activity, mainly through transcriptional up-regulation of the human telomerase catalytic subunit (hTERT). The HBZ (HTLV-1 bZIP) protein coded by the minus strand of HTLV-1 genome and expressed in ATL cells has been shown to increase the transcriptional activity of JunD, an AP-1 protein. The presence of several AP-1 binding sites in the hTERT promoter led us to investigate whether HBZ regulates hTERT gene transcription.

Results

Here, we demonstrate using co-transfection assays that HBZ in association with JunD activates the hTERT promoter. Interestingly, the -378/+1 proximal region, which does not contain any AP-1 site was found to be responsible for this activation. Furthermore, an increase of hTERT transcripts was observed in cells co-expressing HBZ and JunD. Chromatin immunoprecipitation (ChIP) assays revealed that HBZ, and JunD coexist in the same DNA-protein complex at the proximal region of hTERT promoter. Finally, we provide evidence that HBZ/JunD heterodimers interact with Sp1 transcription factors and that activation of hTERT transcription by these heterodimers is mediated through GC-rich binding sites for Sp1 present in the proximal sequences of the hTERT promoter.

Conclusion

These observations establish for the first time that HBZ by intervening in the re-activation of telomerase, may contribute to the development and maintenance of the leukemic process.