bluuebunny/crossref_metadata_2025 huggingface dataset:
Possibilities of the Utilization of Ferritic Nitrocarburizing on Case-Hardening Steels
Authors: Prochazka Jiri , Pokorny Zdenek , Jasenak Jozef , Majerik Jozef , Neumann Vlastimil
Published: July 2021

This paper is devoted to the possibilities of the utilization of chosen chemical heat treatment technologies on steels used for manufacturing highly stressed components of military vehicles and weapons systems. The technologies chosen for this research are plasma ferritic nitrocarburizing and ferritic nitrocarburizing in a gaseous atmosphere. These technologies were applied on a steel equivalent 1.5752 (i.e., CSN 41 6426), which is suitable for carburizing. Chemical composition of the steel was verified by optical emission spectrometry. An observation of a microstructure and an assessment of the parameters of obtained white layers were performed by optical microscopy. Morphology and porosity of the surface were observed by electron microscopy. The depth of diffusion layers was evaluated in accordance with ISO 18203:2016(E) from the results of microhardness measurements. A friction coefficient was obtained as a result of measurements in accordance with a linearly reciprocating ball-on-flat sliding wear method. Wear resistance was assessed by employing the scratch test method and a profilometry. The profilometry was also utilized for surface roughness assessment. It was proved that both tested chemical heat treatment technologies are suitable for surface treatment of the selected steel. Both technologies, ferritic nitrocarburizing in plasma and a gaseous atmosphere, are beneficial for the improvement of surface properties and could lead to a suppression of geometrical deformation in comparison with frequently utilized carburizing. Moreover, the paper presents a procedure that creates a white layer-less ferritic nitrocarburized surface by utilizing an appropriate modification of chemical heat treatment parameters, thus subsequent machining is no longer required.
Large Deformation and Energy Absorption Behaviour of Perforated Hollow Sphere Structures under Quasi-Static Compression
Authors: Dai Meiling , Liang Junping , Cheng Cheng , Wu Zhiwen , Lu Jiexun , Deng Jiyu
Published: July 2021

Hollow sphere structures with perforations (PHSSs) in three different arrangements (simple cubic (SC), body-centred cubic (BCC), and face-centred cubic (FCC)) were fabricated through three-dimensional (3D) printing, and the mechanical behaviours of these PHSSs under quasi-static compression were investigated experimentally and numerically. The results indicated that under uniaxial compression, the PHSSs mainly undergo three stages, i.e., a linear elastic stage, a large deformation or plateau stage, and a densification stage. During the stage of large deformation, the SC and BCC PHSSs experience a preliminary compaction sub-stage after layer-by-layer buckling, while for the FCC PHSS, layer-by-layer collapse and compaction are the dominant deformation behaviours. A numerical simulation was employed to study the mechanical properties of PHSSs with different geometric parameters under quasi-static compression and to explore the effect of the wall thickness, hole diameter, and sphere arrangement on the first peak stress, plateau stress, and specific energy absorption (SEA) of the PHSSs. The results reveal that the geometric parameters have a significant impact on the large deformation behaviour and energy absorption capacity of PHSSs. The presented PHSS is also proven to be much lighter than traditional metallic hollow sphere structure (MHSS) and has higher specific strength and SEA.
Protection of Concrete Structures: Performance Analysis of Different Commercial Products and Systems
Authors: Coffetti Denny , Crotti Elena , Gazzaniga Gabriele , Gottardo Roberto , Pastore Tommaso , Coppola Luigi
Published: July 2021

The increasing demand for reconstructions of concrete structures and the wide availability on the market of surface protective products and systems could lead to misunderstandings in the decision of the most effective solution. Surface protectors have become increasingly widespread in recent years in concrete restoration interventions thanks to their properties: they are able to protect the substrate from aggressive agents and consequently extend the useful life of the structures. The aim of this article is first of all to present the surface protective treatments available on the market, outlining their strengths and weaknesses. Subsequently, a characterization of seven different commercial coatings for reinforced-concrete structures is provided, taking into account chemical nature, fields of use and effectiveness, both in terms of physic and elastic performance and resistance to aggressive agents that undermine the durability of the treated concrete elements.
TiO2@Cu2O n-n Type Heterostructures for Photochemistry
Authors: Trenczek-Zajac Anita , Banas-Gac Joanna , Radecka Marta
Published: July 2021

A TiO2@Cu2O semiconductor heterostructure with better photochemical response compared to TiO2 was obtained using an electrochemical deposition method of Cu2O on the surface of TiO2 nanotubes. The choice of 1D nanotubes was motivated by the possibility of achieving fast charge transfer, which is considered best suited for photochemical applications. The morphology and structural properties of the obtained heterojunction were determined using standard methods —SEM and Raman spectroscopy. Analysis of photoelectrochemical properties showed that TiO2@Cu2O heterostructures exhibit better properties resulting from an interaction with sunlight than TiO2. A close relationship between the morphology of the heterostructures and their photoproperties was also demonstrated. Investigations representing a combination of photoelectrochemical cells for hydrogen production and photocatalysis—photoelectrocatalysis—were also carried out and confirmed the observations on the photoproperties of heterostructures. Analysis of the Mott–Schottky plots as well as photoelectrochemical measurements (Iph-V, Iph-t) showed that TiO2 as well as, unusually, Cu2O exhibit n-type conductivity. On this basis, a new energy diagram of the TiO2@Cu2O system was proposed. It was found that TiO2@Cu2O n-n type heterostructure prevents the processes of photocorrosion of copper(I) oxide contained in a TiO2-based heterostructure.
Nano Calcium Carbonate (CaCO3) as a Reliable, Durable, and Environment-Friendly Alternative to Diminishing Fly Ash
Authors: Poudyal Lochana , Adhikari Kushal , Won Moon
Published: July 2021

Fly ash is widely used in the cement industry to improve the performance and durability of concrete. The future availability of fly ash, however, is a concern, as most countries are inclining towards renewable energy sources as opposed to fossil fuels. Additional concerns have been raised regarding the impact of strict environmental regulations on fly ash quality and variability. This paper, therefore, evaluates if nano calcium carbonate (nano CaCO3) can be used as an alternative to fly ash. This paper presents comprehensive testing results (fresh, hardened, and durability) for OPC (Ordinary Portland Cement) and PLC (Portland Limestone Cement) concretes with 1% nano CaCO3 and compares them to those for concretes with fly ash (both Class F and C). Compared to concretes with fly ash, OPC and PLC with nano CaCO3 presented improved testing results in most cases, including later age strength, permeability, and scaling resistance. As nanotechnology in concrete is a relatively new topic, more research on the efficient use of nanotechnology, such as for proper dispersion of nano CaCO3 in the concrete, has potential to offer increased benefits. Further, nano CaCO3 is environmentally and economically viable, as it has the potential to be produced within the cement plant while utilizing waste CO2 and generating economic revenue to the industry. Thus, nano CaCO3 has the potential to serve as an alternative to fly ash in all beneficial aspects—economic, environmental, and technical.
Selecting Bloggers for Hotels via an Innovative Mixed MCDM Model
Authors: Tsai Jung-Fa , Wang Chin-Po , Chang Kuei-Lun , Hu Yi-Chung
Published: July 2021

The global coronavirus disease 2019 (COVID-19) outbreak had a great impact on the tourism industry. Numerous hotels have ceased operations. Because of the increasing influence of blogs, various industries have adopted blogs as a publicity and marketing strategy. Companies utilize consumers’ trust and loyalty toward bloggers to effectively contact them. Hence, bloggers play a crucial role in the hotel industry. No past study has researched blogger selection by hotel managers. In this study, an innovative mixed multiple-criteria decision-making (MCDM) model including importance-performance analysis (IPA), analytic hierarchy process (AHP), and technique for order preference by similarity to ideal solution (TOPSIS) is established to assist hotel managers in selecting bloggers. We firstly collect the selection criteria via interviews with hotel managers and a review of literature on blogger selection. Messages with stick are understood, remembered, and have an enduring influence on opinions and behavior. Hence, we also introduce the concept of stick to the selection criteria. Based on IPA and the literature review, a hierarchical structure for blogger selection is constructed. Then, AHP and TOPSIS are integrated to assist the case company managers to select suitable bloggers.
A New Forward–Backward Algorithm with Line Searchand Inertial Techniques for Convex Minimization Problems with Applications
Authors: Chumpungam Dawan , Sarnmeta Panitarn , Suantai Suthep
Published: July 2021

For the past few decades, various algorithms have been proposed to solve convex minimization problems in the form of the sum of two lower semicontinuous and convex functions. The convergence of these algorithms was guaranteed under the L-Lipschitz condition on the gradient of the objective function. In recent years, an inertial technique has been widely used to accelerate the convergence behavior of an algorithm. In this work, we introduce a new forward–backward splitting algorithm using a new line search and inertial technique to solve convex minimization problems in the form of the sum of two lower semicontinuous and convex functions. A weak convergence of our proposed method is established without assuming the L-Lipschitz continuity of the gradient of the objective function. Moreover, a complexity theorem is also given. As applications, we employed our algorithm to solve data classification and image restoration by conducting some experiments on these problems. The performance of our algorithm was evaluated using various evaluation tools. Furthermore, we compared its performance with other algorithms. Based on the experiments, we found that the proposed algorithm performed better than other algorithms mentioned in the literature.
Fast Leak-Proof, Intraumbilical, Single-Incision Laparoscopic Ovarian Cystectomy for Huge Ovarian Masses: “Hybrid Cystectomy and Reimplantation” Method
Authors: Lee Sa Ra
Published: July 2021

Background and Objectives: To introduce a new technique for fast leakage-proof, intraumbilical, single-incision laparoscopic ovarian cystectomy for huge ovarian masses (>10 cm) Materials and Methods: Seven consecutive, reproductive-aged women, including three adolescents, with huge ovarian masses (mature cystic teratoma, n = 4; endometrioma, n = 2; and mucinous cystadenoma, n = 1) who underwent transumbilical single-incision ovarian cystectomy with the new “hybrid cystectomy and reimplantation” method were included. The procedure was: (1) trans-umbilical single-incision laparoscopy; (2) inspection of the pelvic cavity and placing the mass in a laparoscopic endo-bag for cystic content leakage prevention; (3) in-bag resection using cold scissors and minimal cauterization of the cystectomy site; (4) in-bag tissue extraction; (5) rapid extracorporeal cystectomy with traction without electrocautery; (6) re-insertion of the retrieved ovarian cortex intracorporeally through the single port, and (7) intracorporeal suture of the retrieved tissue to the in situ ovary. Results: The mean patient age was 24.71 ± 6.56 (range 17–37) years and the mean maximal diameter of the masses was 17.71 ± 2.86 (range 13–22) cm. There was no case of unintended intracorporeal cyst rupture and no need for copious irrigation for washing and suctioning the leaked mass content. The mean total operating time was 76.42 ± 6.39 (range 65–85) min, the total volume of saline used for irrigation was 814.28 ± 331.35 (range 500–1500) mL, and the estimated blood loss was 107.14 ± 47.72 (range 50–200) mL. There were no perioperative complications. All patients except the two endometriosis patients had regular, normal menstruation. Conclusions: Our preliminary findings were encouraging in terms of the safety and efficiency of the new method. Future trials need to elucidate the benefits of this method in terms of fertility preservation.
Gut Microbiota Regulates the Interaction between Diet and Genetics to Influence Glucose Tolerance
Authors: Franson Jeralyn , Grose Julianne , Larson Kaitlyn , Bridgewater Laura
Published: July 2021

Background: Metabolic phenotypes are the result of an intricate interplay between multiple factors, including diet, genotype, and the gut microbiome. Per–Arnt–Sim (PAS) kinase is a nutrient-sensing serine/threonine kinase, whose absence (PASK−/−) protects against triglyceride accumulation, insulin resistance, and weight gain on a high-fat diet; conditions that are associated with dysbiosis of the gut microbiome. Methods: Herein, we report the metabolic effects of the interplay of diet (high fat high sugar, HFHS), genotype (PASK−/−), and microbiome (16S sequencing). Results: Microbiome analysis identified a diet-induced, genotype-independent forked shift, with two discrete clusters of HFHS mice having increased beta and decreased alpha diversity. A “lower” cluster contained elevated levels of Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria and Defferibacteres, and was associated with increased weight gain, glucose intolerance, triglyceride accumulation, and decreased claudin-1 expression. Genotypic effects were observed within the clusters, lower cluster PASK−/− mice displayed increased weight gain and decreased triglyceride accumulation, whereas upper PASK−/− were resistant to decreased claudin-1. Conclusions: These results confirm previous reports that PAS kinase deficiency can protect mice against the deleterious effects of diet, and they suggest that microbiome imbalances can override protection. In addition, these results support a healthy diet for beneficial microbiome maintenance and suggest microbial culprits associated with metabolic disease.
Effect of Intermetallic Compound Bridging on the Cracking Resistance of Sn2.3Ag Microbumps with Different UBM Structures under Thermal Cycling
Authors: Mo Chun-Chieh , Tran Dinh-Phuc , Juang Jing-Ye , Chen Chih
Published: July 2021

In this study, the effect of intermetallic compound (IMC) bridging on the cracking resistance of microbumps with two different under bump metallization (UBM) systems, Cu/solder/Cu and Cu/solder/Ni, under a thermal cycling test (TCT) is investigated. The height of the Sn2.3Ag solders was ~10 µm, which resembles that of the most commonly used microbumps. We adjusted the reflow time to control the IMC bridging level. The samples with different bridging levels were tested under a TCT (−55–125 °C). After 1000 and 2000 TCT cycles (30 min/cycle), the samples were then polished and characterized using a scanning electron microscope (SEM). Before IMC bridging, various cracks in both systems were observed at the IMC/solder interfaces after the 1000-cycle tests. The cracks propagated as cyclic shapes from the sides to the center and became more severe as the thermal cycle was increased. With IMC bridging, we could not observe any further failure in all the samples even when the thermal cycle was up to 2000. We discovered that IMC bridging effectively suppressed crack formation in microbumps under TCTs.
New Ti–35Nb–7Zr–5Ta Alloy Manufacturing by Electron Beam Melting for Medical Application Followed by High Current Pulsed Electron Beam Treatment
Authors: Surmeneva Maria , Grubova Irina , Glukhova Natalia , Khrapov Dmitriy , Koptyug Andrey , Volkova Anastasia , Ivanov Yurii , Cotrut Cosmin Mihai , Vladescu Alina , Teresov Anton , Koval Nikolay , Tyurin Alexander , Surmenev Roman
Published: July 2021

High-current pulsed electron-beam (PEB) treatment was applied as a surface finishing procedure for Ti–35Nb–7Zr–5Ta (TNZT) alloy produced by electron beam melting (EBM). According to the XRD results the TNZT alloy samples before and after the PEB treatment have shown mainly the single body-centered cubic (bcc) β-phase microstructures. The crystallite size, dislocation density, and microstrain remain unchanged after the PEB treatment. The investigation of the texture coefficient at the different grazing angle revealed the evolution of the crystallite orientations at the re-melted zone formed at the top of the bulk samples after the PEB treatment. The top-view SEM micrographs of the TNZT samples treated by PEB exhibited the bcc β-phase grains with an average size of ~85 μm. TEM analysis of as-manufactured TNZT alloy revealed the presence of the equiaxed β-grains with the fine dispersion of nanocrystalline α and NbTi4 phases together with β-Ti twins. Meanwhile, the β phase regions free of α phase precipitation are observed in the microstructure after the PEB irradiation. Nanoindentation tests revealed that the surface mechanical properties of the melted zone were slightly improved. However, the elastic modulus and microhardness in the heat-affected zone and the deeper regions of the sample were not changed after the treatment. Moreover, the TNZT alloy in the bulk region manufactured by EBM displayed no significant change in the corrosion resistance after the PEB treatment. Hence, it can be concluded that the PEB irradiation is a viable approach to improve the surface topography of EBM-manufactured TNZT alloy, while the most important mechanical parameters remain unchanged.
Survival Rate and Deformation of External Hexagon Implants with One-Piece Zirconia Crowns
Authors: Bottino Marco Antonio , de Oliveira Flávio Rosa , Sabino Clarice Ferreira , Dinato José Cícero , Silva-Concílio Laís Regiane , Tribst João Paulo Mendes
Published: July 2021

This study aimed to evaluate the survival rates of several external hexagon implants directly connected to zirconia crowns after thermomechanical fatigue. The deformation of the hexagons and the integrity of zirconia crowns were also evaluated. A monolithic zirconia crown (Y-TZP) and four different external hexagon dental implants (n = 10, N = 40) were mounted together and embedded in polyurethane. The specimens were subjected to thermomechanical cycling for 2.5 × 106 cycles, at 3.0 Hz frequency, at 200 N loading. The interface of the implant/zirconia crown system, zirconia crowns integrity before and after cycling, and the implant hexagon surface were evaluated under stereomicroscopy and SEM. A nanohardness analysis was performed to verify the hardness of zirconia and implants. Statistical analysis was performed using the Kaplan-Meier test, Multi-Sample Survival Tests, Logrank Test, (p = 0.05). The data did not show significant differences in the survival rates of different implant groups. However, some crowns presented fractures (16.67%) and the external hexagon region of the implants presented plastic deformations (100%). During chewing simulation, the interface between titanium implant and zirconia abutment can promote plastic deformation in the metal and surface defects in the ceramic. In addition, the types of interface defects can be affected by the external hexagon design.
In Situ Medium Entropy Intermetallic Reinforced Composite Coating Fabricated by Additive Manufacturing
Authors: Ostovari Moghaddam Ahmad , Shaburova Nataliya Aleksandrovna , Samodurova Marina Nikolaevna , Latfulina Yuliya Sergeevna , Mikhailov Dmitry Vyacheslavovich , Trofimov Evgeny Alekseevich
Published: July 2021

The possibility of stabilizing different amounts of medium-entropy intermetallic compounds (MEIMCs) within a multicomponent matrix using laser cladding is demonstrated. The results indicated that MEIMC with a B2 structure could be successfully formed within a multicomponent BCC matrix during laser cladding of a proper ratio of Al, Fe, Co, Cu, Mn, and Ni powders. Two coatings with different contents of MEIMC were fabricated by changing the feeding rate of the powder mixture. Based on the X-ray diffraction (XRD) and energy dispersive spectroscopy (EDS) analyses, the Al-rich intermetallic particles were qualitatively identified as (Fe0.55Co0.18Cu0.22Ni0.03Mn0.02)Al MEIMC. It was also found that the feeding rate affects the content of MEIMC, and consequently, the grain structure and microhardness values. Finally, we propose MEIMC-reinforced alloys as a more effective alternative system to be used for fabricating high-performance coatings using laser cladding.
Assessment of Antioxidant and Antimutagenic Properties of Red and White Wine Extracts In Vitro
Authors: Tekos Fotios , Makri Sotiria , Skaperda Zoi-Vasiliki , Patouna Anastasia , Terizi Kallirroi , Kyriazis Ioannis D. , Kotseridis Yorgos , Mikropoulou Eleni Vaskani , Papaefstathiou Georgios , Halabalaki Maria , Demetrios Kouretas
Published: July 2021

Wine is an alcoholic beverage of complex composition obtained through the fermentation of grape must. The consumption of wine has already been associated with a multitude of beneficial effects due to its high polyphenolic content. In this study, four Greek emblematic wines from two red (i.e., Xinomavro and Agiorgitiko) and two white (i.e., Assyrtiko and Malagouzia) varieties were analyzed for the estimation of their antioxidant profiles. To address this question, we assessed their ability to scavenge both synthetic and endogenous free radicals, such as DPPH•, ABTS+•, OH•, O2−, their potential reducing power, and their antimutagenic and antigenotoxic properties. All varieties exhibited potent antioxidant activity, as indicated by the results of methods above, with the red wines appearing more effective than the white ones regarding antioxidant capacity. Our small-scale study is the first to reveal that these wine varieties may have the ability to scavenge the most reactive endogenous radicals. In the future, this finding must be accompanied by larger studies to fill a knowledge gap in the scientific literature concerning a holistic approach of the in vitro antioxidant action of plant polyphenolic compounds. Conclusively, we believe that wines possess high bioactivity that allow them to settle in the industry of food additives and medicinal products.
Memristor-CMOS Hybrid Neuron Circuit with Nonideal-Effect Correction Related to Parasitic Resistance for Binary-Memristor-Crossbar Neural Networks
Authors: Nguyen Tien Van , An Jiyong , Min Kyeong-Sik
Published: July 2021

Voltages and currents in a memristor crossbar can be significantly affected due to nonideal effects such as parasitic source, line, and neuron resistance. These nonideal effects related to the parasitic resistance can cause the degradation of the neural network’s performance realized with the nonideal memristor crossbar. To avoid performance degradation due to the parasitic-resistance-related nonideal effects, adaptive training methods were proposed previously. However, the complicated training algorithm could add a heavy computational burden to the neural network hardware. Especially, the hardware and algorithmic burden can be more serious for edge intelligence applications such as Internet of Things (IoT) sensors. In this paper, a memristor-CMOS hybrid neuron circuit is proposed for compensating the parasitic-resistance-related nonideal effects during not the training phase but the inference one, where the complicated adaptive training is not needed. Moreover, unlike the previous linear correction method performed by the external hardware, the proposed correction circuit can be included in the memristor crossbar to minimize the power and hardware overheads for compensating the nonideal effects. The proposed correction circuit has been verified to be able to restore the degradation of source and output voltages in the nonideal crossbar. For the source voltage, the average percentage error of the uncompensated crossbar is as large as 36.7%. If the correction circuit is used, the percentage error in the source voltage can be reduced from 36.7% to 7.5%. For the output voltage, the average percentage error of the uncompensated crossbar is as large as 65.2%. The correction circuit can improve the percentage error in the output voltage from 65.2% to 8.6%. Almost the percentage error can be reduced to ~1/7 if the correction circuit is used. The nonideal memristor crossbar with the correction circuit has been tested for MNIST and CIFAR-10 datasets in this paper. For MNIST, the uncompensated and compensated crossbars indicate the recognition rate of 90.4% and 95.1%, respectively, compared to 95.5% of the ideal crossbar. For CIFAR-10, the nonideal crossbars without and with the nonideal-effect correction show the rate of 85.3% and 88.1%, respectively, compared to the ideal crossbar achieving the rate as large as 88.9%.
Multiple Potential Plant Growth Promotion Activities of Endemic Streptomyces spp. from Moroccan Sugar Beet Fields with Their Inhibitory Activities against Fusarium spp.
Authors: Aallam Yassine , Maliki Bouchra El , Dhiba Driss , Lemriss Sanaa , Souiri Amal , Haddioui Abdelmajid , Tarkka Mika , Hamdali Hanane
Published: July 2021

The characterized 10 Streptomyces isolates were previously selected by their abilities to solubilize phosphates. To investigate whether these isolates represent multifaceted plant growth-promoting rhizobacteria (PGPR), their potassium-solubilizing, auxin-producing and inhibitory activities were determined. The 10 Streptomyces spp. yielded a variable biomass in the presence of insoluble orthoclase as the sole potassium (K) source, indicating that they were able to extract different amounts of K from this source for their own growth. Three strains (AZ, AYD and DE2) released soluble K from insoluble orthoclase in large amounts into the culture broth. The production levels ranged from 125.4 mg/L to 216.6 mg/L after 5 days of culture. Only two strains, Streptomyces enissocaesilis (BYC) and S. tunisiensis (AI), released a larger amount of soluble K from orthoclase and yielded much more biomass. This indicated that the rate of K released from this insoluble orthoclase exceeded its consumption rate for bacterial growth and that some strains solubilized K more efficiently than others. The results also suggest that the K solubilization process of AZ, AYD and DE2 strains, the most efficient K-solubilizing strains, involves a slight acidification of the medium. Furthermore, these 10 Streptomyces spp. were able to secrete indole acetic acid (IAA) in broth medium and ranged from 7.9 ± 0.1 µg/mL to 122.3 ± 0.1 µg/mL. The results of the antibiosis test proved the potential of the 10 tested strains to limit the growth of fungi and bacteria. In dual culture, S. bellus (AYD) had highest inhibitory effect against the three identified fungal causal agents of root rot of sugar beet: Fusarium equiseti and two F. fujikuroi at 55, 43 and 36%, respectively. Streptomyces enissocaesilis (BYC), S. bellus (AYD) and S. saprophyticus (DE2) exhibited higher multifaceted PGPR with their potassium-solubilizing, auxin-producing and inhibitory activities, which could be expected to lead to effectiveness in field trials of sugar beet.
Nutrient Acquisition and Attachment Strategies in Basal Lineages: A Tough Nut to Crack in the Evolutionary Puzzle of Apicomplexa
Authors: Valigurová Andrea , Florent Isabelle
Published: July 2021

Apicomplexa are unicellular eukaryotes that parasitise a wide spectrum of invertebrates and vertebrates, including humans. In their hosts, they occupy a variety of niches, from extracellular cavities (intestine, coelom) to epicellular and intracellular locations, depending on the species and/or developmental stages. During their evolution, Apicomplexa thus developed an exceptionally wide range of unique features to reach these diversified parasitic niches and to survive there, at least long enough to ensure their own transmission or that of their progeny. This review summarises the current state of knowledge on the attachment/invasive and nutrient uptake strategies displayed by apicomplexan parasites, focusing on trophozoite stages of their so far poorly studied basal representatives, which mostly parasitise invertebrate hosts. We describe their most important morphofunctional features, and where applicable, discuss existing major similarities and/or differences in the corresponding mechanisms, incomparably better described at the molecular level in the more advanced Apicomplexa species, of medical and veterinary significance, which mainly occupy intracellular niches in vertebrate hosts.
Current Epidemiological Characteristics of Imported Malaria, Vector Control Status and Malaria Elimination Prospects in the Gulf Cooperation Council (GCC) Countries
Authors: Iqbal Jamshaid , Ahmad Suhail , Sher Ali , Al-Awadhi Mohammad
Published: July 2021

Malaria is the most common vector-borne parasitic infection causing significant human morbidity and mortality in nearly 90 tropical/sub-tropical countries worldwide. Significant differences exist in the incidence of malaria cases, dominant Plasmodium species, drug-resistant strains and mortality rates in different countries. Six Gulf Cooperation Council (GCC) countries (Bahrain, Kuwait, Qatar, Oman, Saudi Arabia and United Arab Emirates, UAE) in the Middle East region with similar climates, population demographics and economic prosperity are aiming to achieve malaria elimination. In this narrative review, all studies indexed in PubMed describing epidemiological characteristics of indigenous and imported malaria cases, vector control status and how malaria infections can be controlled to achieve malaria elimination in GCC countries were reviewed and discussed. These studies have shown that indigenous malaria cases are absent in Bahrain, Kuwait, Qatar and UAE and have progressively declined in Oman and Saudi Arabia. However, imported malaria cases continue to occur as GCC countries have large expatriate populations originating from malaria-endemic countries. Various malaria control and prevention strategies adopted by GCC countries including more stringent measures to reduce the likelihood of importing malaria cases by prior screening of newly arriving expatriates and vector elimination programs are likely to lead to malaria elimination in this region.
Insights from Bacteroides Species in Children with Type 1 Diabetes
Authors: Matos José , Matos Isabel , Calha Manuela , Santos Pedro , Duarte Isabel , Cardoso Yameric , Faleiro Maria Leonor
Published: July 2021

In our previous study the enrichment of the intestinal proteome of type 1 diabetes (T1D) children with Bacteroides proteins was observed, which led us to our current study that aimed to isolate and characterize Bacteroides species from fecal samples of T1D and control children. Repetitive sequence-based PCR (rep-PCR) was used for typing the isolated Bacteroides species. The antibiotic susceptibility and mucinolytic activity of the isolates was determined. The quantification of specific bacterial groups in the fecal samples was determined by qPCR. The ability to adhere and invade the human colonic cell line HT29-MTX-E12 of strains of P. dorei, B. uniformis and P. distasonis was determined and their whole genome sequencing was performed. The results showed similar numbers of Bacteroides species in T1D and control samples, but unique Bacteroides species and a higher recovery of P. distasonis from T1D samples was observed. Rep-PCR grouped the different Bacteroides species, but no discrimination by origin was achieved. T1D children showed a significant increase in Proteobacteria and a depletion in Lactobacillus sp. All tested P. dorei, B. uniformis and P. distasonis were able to adhere to HT29-MTX-E12 cells but significant differences (p < 0.05) in the ability to invade was observed. The highest ability to invade was exhibited by P. distasonis PtF D14MH1 and P. dorei PtFD16P1, while B. uniformis strains were unable to invade. The damage to tight junctions was also observed. The presence of Lactobacillus sp. inhibited the invasion ability of P. distasonis PtF D14MH1 but not P. dorei PtFD16P1. Sequences of agonist peptides of the human natural preproinsulin and the insulin B chain insB:9-23 peptide mimics were identified. The results reported in our study stresses the continued efforts required to clarify the link between T1D and gut microbiota.
On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation
Authors: Lukić Miodrag J. , Lücke Felix , Ilić Teodora , Petrović Katharina , Gebauer Denis
Published: July 2021

Nucleation of minerals in the presence of additives is critical for achieving control over the formation of solids in biomineralization processes or during syntheses of advanced hybrid materials. Herein, we investigated the early stages of Fe(III) (oxy)(hydr)oxide formation with/without polyglutamic acid (pGlu) at low driving force for phase separation (pH 2.0 to 3.0). We employed an advanced pH-constant titration assay, X-ray diffraction, thermal analysis with mass spectrometry, Fourier Transform infrared spectroscopy, and scanning electron microscopy. Three stages were observed: initial binding, stabilization of Fe(III) pre-nucleation clusters (PNCs), and phase separation, yielding Fe(III) (oxy)(hydr)oxide. The data suggest that organic–inorganic interactions occurred via binding of olation Fe(III) PNC species. Fourier Transform Infrared Spectroscopy (FTIR) analyses revealed a plausible interaction motif and a conformational adaptation of the polypeptide. The stabilization of the aqueous Fe(III) system against nucleation by pGlu contrasts with the previously reported influence of poly-aspartic acid (pAsp). While this is difficult to explain based on classical nucleation theory, alternative notions such as the so-called PNC pathway provide a possible rationale. Developing a nucleation theory that successfully explains and predicts distinct influences for chemically similar additives like pAsp and pGlu is the Holy Grail toward advancing the knowledge of nucleation, early growth, and structure formation.
Chalcogen Bonding in Co-Crystals: Activation through 1,4-Perfluorophenylene vs. 4,4′-Perfluorobiphenylene Cores
Authors: Dhaka Arun , Jeannin Olivier , Aubert Emmanuel , Espinosa Enrique , Fourmigué Marc
Published: July 2021

The ability of alkylseleno/alkyltelluroacetylenes such as bis(selenomethylethynyl)-perfluorobenzene (4F-Se) to act as a ditopic chalcogen bond (ChB) donor in co-crystals with ditopic Lewis bases such as 4,4′-bipyridine is extended here to the octafluorobiphenylene analog, 4,4′-bis(selenomethylethynyl)-perfluorobiphenyl (8F-Se), with the more electron-rich 4,4′-bipyridylethane (bpe), showing in the 1:1 (8F-Se)•(bpe) co-crystal a shorter and more linear C−Se•••N ChB interaction than in (4F-Se)•(bpe), with Se•••N distances down to 2.958(2) Å at 150 K, i.e., a reduction ratio of 0.85 vs. the van der Waals contact distance.
Effects of Green Tea Powder, Pomegranate Peel Powder, Epicatechin and Punicalagin Additives on Antimicrobial, Antioxidant Potential and Quality Properties of Raw Meatballs
Authors: Demir Tuğba
Published: July 2021

Alternative technologies, which have been developed in order to meet the consumers’ demand for nourishing and healthy meat and meat products, are followed by the food industry. In the present study, it was determined, using the HPLC method, that green tea contains a high level of epicatechin (EP) under optimal conditions and that pomegranate peel contains a high level of punicalagin (PN). Green tea, pomegranate peel, EP and PN were added to meatballs at different concentrations in eight groups. The antioxidant capacities of extracts were measured. The antimicrobial activity was examined for 72 h using three different food pathogens. The highest level of antimicrobial activity was achieved in the 1% punicalagin group, whereas the minimum inhibition concentration (L. monocytogenes, S. typhimurium) was found to be 1.87 mg/mL. A statistically significant decrease was found in FFA, POV and TBARS levels of meatballs on different days of storage (p < 0.05). When compared to the control group, the bioactive compounds preserved the microbiological and chemical properties of meatballs during storage at +4 °C (14 days). It was concluded that the extracts with high EP and PN concentrations can be used as bio-preservative agents for meat and meat products.
The Tannins from Sanguisorba officinalis L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS2 Analysis
Authors: Tu Jingjing , Li Qiaoling , Zhou Benhong
Published: July 2021

Sanguisorba tannins are the major active ingredients in Sanguisorba ofJicinalis L. (Rosaceae), one of the most popular herbal medicines in China, is widely prescribed for hemostasis. In this study, three kinds of tannins extract from Sanguisorba officinalis L. (Rosaceae), and the metabolites in vivo and in vitro were detected and identified by high-pressure liquid chromatography, coupled with linear ion trap orbitrap tandem mass spectrometry (HPLC–LTQ–Orbitrap). For in vivo assessment, the rats were administered at a single dose of 150 mg/kg, after which 12 metabolites were found in urine, 6 metabolites were found in feces, and 8 metabolites were found in bile, while metabolites were barely found in plasma and tissues. For in vitro assessment, 100 μM Sanguisorba tannins were incubated with rat liver microsomes, liver cytosol, and feces, after which nine metabolites were found in intestinal microbiota and five metabolites were found in liver microsomes and liver cytosol. Moreover, the metabolic pathways of Sanguisorba tannins were proposed, which shed light on their mechanism.
Exploration of the Antimicrobial Effects of Benzothiazolylthiazolidin-4-One and In Silico Mechanistic Investigation
Authors: Haroun Michelyne , Tratrat Christophe , Petrou Anthi , Geronikaki Athina , Ivanov Marija , Ćirić Ana , Soković Marina , Nagaraja Sreeharsha , Venugopala Katharigatta Narayanaswamy , Balachandran Nair Anroop , Elsewedy Heba S. , Kochkar Hafedh
Published: July 2021

Background: Infectious diseases still affect large populations causing significant morbidity and mortality. Bacterial and fungal infections for centuries were the main factors of death and disability of millions of humans. Despite the progress in the control of infectious diseases, the appearance of resistance of microbes to existing drugs creates the need for the development of new effective antimicrobial agents. In an attempt to improve the antibacterial activity of previously synthesized compounds modifications to their structures were performed. Methods: Nineteen thiazolidinone derivatives with 6-Cl, 4-OMe, 6-CN, 6-adamantan, 4-Me, 6-adamantan substituents at benzothiazole ring were synthesized and evaluated against panel of four bacterial strains S. aureus, L. monocytogenes, E. coli and S. typhimirium and three resistant strains MRSA, E. coli and P. aeruginosa in order to improve activity of previously evaluated 6-OCF3-benzothiazole-based thiazolidinones. The evaluation of minimum inhibitory and minimum bactericidal concentration was determined by microdilution method. As reference compounds ampicillin and streptomycin were used. Results: All compounds showed antibacterial activity with MIC in range of 0.12–0.75 mg/mL and MBC at 0.25–>1.00 mg/mL The most active compound among all tested appeared to be compound 18, with MIC at 0.10 mg/mL and MBC at 0.12 mg/mL against P. aeruginosa. as well as against resistant strain P. aeruginosa with MIC at 0.06 mg/mL and MBC at 0.12 mg/mL almost equipotent with streptomycin and better than ampicillin. Docking studies predicted that the inhibition of LD-carboxypeptidase is probably the possible mechanism of antibacterial activity of tested compounds. Conclusion: The best improvement of antibacterial activity after modifications was achieved by replacement of 6-OCF3 substituent in benzothiazole moiety by 6-Cl against S. aureus, MRSA and resistant strain of E. coli by 2.5 folds, while against L. monocytogenes and S. typhimirium from 4 to 5 folds.
Recent Advances in Chemistry of Unsymmetrical Phosphorus-Based Pincer Nickel Complexes: From Design to Catalytic Applications
Authors: Gafurov Zufar N. , Kantyukov Artyom O. , Kagilev Alexey A. , Kagileva Alina A. , Sakhapov Il’yas F. , Mikhailov Ilya K. , Yakhvarov Dmitry G.
Published: July 2021

Pincer complexes play an important role in organometallic chemistry; in particular, their use as homogeneous catalysts for organic transformations has increased dramatically in recent years. The high catalytic activity of such bis-cyclometallic complexes is associated with the easy tunability of their properties. Moreover, the phosphorus-based unsymmetrical pincers showed higher catalytic activity than the corresponding symmetrical analogues in several catalytic reactions. However, in modern literature, an increasing interest in the development of catalysts based on non-precious metals is observed. For example, nickel, which is an affordable and sustainable analogue of platinum and palladium, known for its low toxicity, has attracted increasing attention in the catalytic chemistry of transition metals in recent years. Thus, this mini-review is devoted to the recent advances in the chemistry of unsymmetrical phosphorus-based pincer nickel complexes, including the ligand design, the synthesis of nickel complexes and their catalytic applications.
Synthetic Data in Quantitative Scanning Probe Microscopy
Authors: Nečas David , Klapetek Petr
Published: July 2021

Synthetic data are of increasing importance in nanometrology. They can be used for development of data processing methods, analysis of uncertainties and estimation of various measurement artefacts. In this paper we review methods used for their generation and the applications of synthetic data in scanning probe microscopy, focusing on their principles, performance, and applicability. We illustrate the benefits of using synthetic data on different tasks related to development of better scanning approaches and related to estimation of reliability of data processing methods. We demonstrate how the synthetic data can be used to analyse systematic errors that are common to scanning probe microscopy methods, either related to the measurement principle or to the typical data processing paths.
Pharmacodynamics of Oral Cholecalciferol in Healthy Individuals with Vitamin D Deficiency: A Randomized Open-Label Study
Authors: Fassio Angelo , Gatti Davide , Rossini Maurizio , Benini Camilla , Fracassi Elena , Bertoldo Eugenia , Viapiana Ombretta , Milleri Stefano , Gatti Matteo , Adami Giovanni
Published: July 2021

Comparative pharmacodynamic (PD) analyses on different dosing schedules for cholecalciferol supplementation are limited. This was an open-label, randomized, parallel-group study involving 75 healthy individuals deficient in vitamin D (baseline 25OHD < 20 ng/mL) receiving oral cholecalciferol with three different dosing regimens: Group A: 10,000 IU/day for 8 weeks followed by 1000 IU/day for 4 weeks; Group B: 50,000 IU/week for 12 weeks and Group C: 100,000 IU every other week for 12 weeks. Regulators of calcium and phosphate homeostasis, bone turnover markers and Wnt inhibitors were measured at baseline, Day 28, 53, 84, and 112. The 1,25OH2D increased at each time point. The increase was greater (p < 0.05) for group A vs. B and C at Day 28, and vs. group B at Day 56. No significant difference among groups was observed for the other biomarkers. The 24,25OH2D remained stable over time. PTH decreased at Day 84 and FGF-23 increased at all time points. CTX-I and PINP increased slightly at Day 28. BALP decreased from Day 56 onward. Dkk-1 increased from Day 56 onward, while sclerostin did not show significant changes. In healthy individuals deficient in vitamin D, vitamin D supplementation exerted effects on multiple regulators of calcium, phosphate and bone metabolism, without marked differences using the three regimens.
Successful Eradication of Porcine Epidemic Diarrhea in an Enzootically Infected Farm: A Two-Year Follow-Up Study
Authors: Jang Guehwan , Park Jonghyun , Lee Changhee
Published: July 2021

Porcine epidemic diarrhea virus (PEDV) has negatively affected the welfare of animals and their productivity in South Korea for three decades. A shortage of effective control measures has led to the virus becoming endemic in domestic pig populations. This study aimed to describe how our intervention measures were implemented for PEDV elimination in an enzootically infected farm. We operated a risk assessment model of PEDV recurrence to obtain information about the virus itself, herd immunity, virus circulation, and biosecurity at the farm. Next, we conducted a four-pillar-based two-track strategy to heighten sow immunity and eradicate the virus, with longitudinal monitoring of immunity and virus circulation, involving strict biosecurity, prime-boost pre-farrow L/K/K immunization, all-in-all-out and disinfection practices in farrowing houses, and disinfection and gilt management in wean-to-finish barns. In particular, we observed a high prevalence and long-term survival of PEDV in slurries, posing a critical challenge to PED eradication and highlighting the necessity for consecutive testing of barn slurry samples and for the management of infected manure to control PEDV. Genetic analysis of PEDVs in this farm indicated that genetic drift continued in the spike gene, with a substitution rate of 1.683 × 10−4 substitutions/site/year. Our study underlines the need for active monitoring and surveillance of PEDV in herds and their environments, along with the coordinated means, to eliminate the virus and maintain a negative herd. The tools described in this study will serve as a framework for regional and national PED eradication programs.
Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
Authors: Lücken Anneke , Wente Nicole , Zhang Yanchao , Woudstra Svenja , Krömker Volker
Published: July 2021

In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rods were differentiated using the direct transfer method in MALDI-TOF MS. C. bovis, C. amycolatum, C. xerosis, and five other species were identified with proportions of 90.1%, 7.7%, and 0.8% for the named species, respectively, and 1.4% for the remaining unnamed species. In addition, somatic cell count (SCC) was determined by flow cytometry. Based on this, the isolates were classified into four udder health groups: “latent infection”, “subclinical mastitis”, “clinical mastitis” and “others”. Approximately 90% of isolates of C. bovis and C. amycolatum were from latently and subclinically infected quarters. Of the C. bovis isolates, 5.8% were obtained from milk samples from clinical mastitis, whereas C. amycolatum was not present in clinical mastitis. The distribution of groups in these two species differed significantly. The geometric mean SCC of all species combined was 76,000 SCC/mL, almost the same as the SCC of C. bovis. With 50,000 SCC/mL, the SCC of C. amycolatum was slightly below the SCC of C. bovis. Through the species-level detection and consideration of SCC performed here, it is apparent that individual species differ in terms of their pathogenicity. Overall, their classification as minor pathogens with an SCC increase is confirmed.