bluuebunny/crossref_metadata_2025 huggingface dataset:
Hepatoprotective Effect of Allium ochotense Extracts on Chronic Alcohol-Induced Fatty Liver and Hepatic Inflammation in C57BL/6 Mice
Authors: Go Min Ji , Kim Jong Min , Lee Hyo Lim , Kim Tae Yoon , Kim Ju Hui , Lee Han Su , Kim In Young , Sim Seon Jeong , Heo Ho Jin
Published: March 2024

This study was performed to investigate the protective effects of Allium ochotense on fatty liver and hepatitis in chronic alcohol-induced hepatotoxicity. The physiological compounds of a mixture of aqueous and 60% ethanol (2:8, w/w) extracts of A. ochotense (EA) were identified as kestose, raffinose, kaempferol and quercetin glucoside, and kaempferol di-glucoside by UPLC Q-TOF MSE. The EA regulated the levels of lipid metabolism-related biomarkers such as total cholesterol, triglyceride, low-density lipoprotein (LDL), and high-density lipoprotein (HDL)-cholesterol in serum. Also, EA ameliorated the levels of liver toxicity-related biomarkers such as glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and total bilirubin in serum. EA improved the antioxidant system by reducing malondialdehyde contents and increasing superoxide dismutase (SOD) levels and reduced glutathione content. EA improved the alcohol metabolizing enzymes such as alcohol dehydrogenase, acetaldehyde dehydrogenase, and cytochrome P450 2E1 (CYP2E1). Treatment with EA alleviated lipid accumulation-related protein expression by improving phosphorylation of AMP-activated protein kinase (p-AMPK) expression levels. Especially, EA reduced inflammatory response by regulating the toll-like receptor–4/nuclear factor kappa-light-chain-enhancer of activated B cells (TLR-4/NF-κB) signaling pathway. EA showed an anti-apoptotic effect by regulating the expression levels of B-cell lymphoma 2 (BCl-2), BCl-2-associated X protein (BAX), and caspase 3. Treatment with EA also ameliorated liver fibrosis via inhibition of transforming growth factor-beta 1/suppressor of mothers against decapentaplegic (TGF-β1/Smad) pathway and alpha-smooth muscle actin (α-SMA). Therefore, these results suggest that EA might be a potential prophylactic agent for the treatment of alcoholic liver disease.
PANC-1 Cell Line as an Experimental Model for Characterizing PIVKA-II Production, Distribution, and Molecular Mechanisms Leading to Protein Release in PDAC
Authors: Farina Antonella , Tartaglione Sara , Preziosi Adele , Mancini Patrizia , Angeloni Antonio , Anastasi Emanuela
Published: March 2024

Pancreatic ductal adenocarcinoma (PDAC) represents a highly aggressive malignancy with a lack of reliable diagnostic biomarkers. Protein induced by vitamin K absence (PIVKA-II) is a protein increased in various cancers (particularly in hepatocellular carcinoma), and it has recently exhibited superior diagnostic performance in PDAC detection compared to other biomarkers. The aim of our research was to identify an in vitro model to study PIVKA-II production, distribution, and release in PDAC. We examined the presence of PIVKA-II protein in a panel of stabilized pancreatic cancer cell lines by Western blot analysis and indirect immunofluorescence (IFA). After quantitative evaluation of PIVKA-II in PaCa 44, H-Paf II, Capan-1, and PANC-1, we adopted the latter as a reference model. Subsequently, we analyzed the effect of glucose addiction on PIVKA-II production in a PANC-1 cell line in vitro; PIVKA-II production seems to be directly related to an increase in glucose concentration in the culture medium. Finally, we evaluated if PIVKA-II released in the presence of increasing doses of glucose is concomitant with the expression of two well-acknowledged epithelial–mesenchymal transition (EMT) markers (Vimentin and Snail). According to our experimental model, we can speculate that PIVKA-II release by PANC-1 cells is glucose-dependent and occurs jointly with EMT activation.
Use of Enzymatically Converted Cell-Free DNA (cfDNA) Data for Copy Number Variation-Linked Fragmentation Analysis Allows for Early Colorectal Cancer Detection
Authors: Černoša Iva , Trincado-Alonso Fernando , Canal-Noguer Pol , Kruusmaa Kristi , Perera-Lluna Alexandre
Published: March 2024

The use of non-invasive liquid biopsy-based cell-free DNA (cfDNA) analysis is an emerging method of cancer detection and intervention. Different analytical methodologies are used to investigate cfDNA characteristics, resulting in costly and long analysis processes needed for combining different data. This study investigates the possibility of using cfDNA data converted for methylation analysis for combining the cfDNA fragment size with copy number variation (CNV) in the context of early colorectal cancer detection. Specifically, we focused on comparing enzymatically and bisulfite-converted data for evaluating cfDNA fragments belonging to chromosome 18. Chromosome 18 is often reported to be deleted in colorectal cancer. We used counts of short and medium cfDNA fragments of chromosome 18 and trained a linear model (LDA) on a set of 2959 regions to predict early-stage (I–IIA) colorectal cancer on an independent test set. In total, 87.5% sensitivity and 92% specificity were obtained on the enzymatically converted libraries. Repeating the same workflow on bisulfite-converted data yielded lower accuracy results with 58.3% sensitivity, implying that enzymatic conversion preserves the cancer fragmentation footprint in whole genome data better than bisulfite conversion. These results could serve as a promising new avenue for the early detection of colorectal cancer using fragmentation and methylation approaches on the same datasets.
Comparative Analysis of the Chloroplast Genomes of Eight Species of the Genus Lirianthe Spach with Its Generic Delimitation Implications
Authors: Wu Tao , Sima Yong-Kang , Chen Shao-Yu , Fu Yu-Pin , Ma Hui-Fen , Hao Jia-Bo , Zhu Yun-Feng
Published: March 2024

Based on Sima and Lu’s system of the family Magnoliaceae, the genus Lirianthe Spach s. l. includes approximately 25 species, each with exceptional landscaping and horticultural or medical worth. Many of these plants are considered rare and are protected due to their endangered status. The limited knowledge of species within this genus and the absence of research on its chloroplast genome have greatly impeded studies on the relationship between its evolution and systematics. In this study, the chloroplast genomes of eight species from the genus Lirianthe were sequenced and analyzed, and their phylogenetic relationships with other genera of the family Magnoliaceae were also elucidated. The results showed that the chloroplast genome sizes of the eight Lirianthe species ranged from 159,548 to 159,833 bp. The genomes consisted of a large single-copy region, a small single-copy region, and a pair of inverted repeat sequences. The GC content was very similar across species. Gene annotation revealed that the chloroplast genomes contained 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes, totaling 130 genes. Codon usage analysis indicated that codon usage was highly conserved among the eight Lirianthe species. Repeat sequence analysis identified 42–49 microsatellite sequences, 16–18 tandem repeats, and 50 dispersed repeats, with microsatellite sequences being predominantly single-nucleotide repeats. DNA polymorphism analysis revealed 10 highly variable regions located in the large single-copy and small single-copy regions, among which rpl32-trnL, petA-psbJ, and trnH-psbA were the recommended candidate DNA barcodes for the genus Lirianthe species. The inverted repeat boundary regions show little variation between species and are generally conserved. The result of phylogenetic analysis confirmed that the genus Lirianthe s. l. is a monophyletic taxon and the most affinal to the genera, Talauma and Dugandiodendron, in Sima and Lu’s system and revealed that the genus Lirianthe s. s. is paraphyletic and the genus Talauma s. l. polyphyletic in Xia’s system, while Magnolia subsection Gwillimia is paraphyletic and subsection Blumiana polyphyletic in Figlar and Nooteboom’s system. Morphological studies found noticeable differences between Lirianthe species in aspects including leaf indumentum, stipule scars, floral orientation, tepal number, tepal texture, and fruit dehiscence. In summary, this study elucidated the chloroplast genome evolution within Lirianthe and laid a foundation for further systematic and taxonomic research on this genus.
NO and H2S Contribute to Crop Resilience against Atmospheric Stressors
Authors: Corpas Francisco J.
Published: March 2024

Atmospheric stressors include a variety of pollutant gases such as CO2, nitrous oxide (NOx), and sulfurous compounds which could have a natural origin or be generated by uncontrolled human activity. Nevertheless, other atmospheric elements including high and low temperatures, ozone (O3), UV-B radiation, or acid rain among others can affect, at different levels, a large number of plant species, particularly those of agronomic interest. Paradoxically, both nitric oxide (NO) and hydrogen sulfide (H2S), until recently were considered toxic since they are part of the polluting gases; however, at present, these molecules are part of the mechanism of response to multiple stresses since they exert signaling functions which usually have an associated stimulation of the enzymatic and non-enzymatic antioxidant systems. At present, these gasotransmitters are considered essential components of the defense against a wide range of environmental stresses including atmospheric ones. This review aims to provide an updated vision of the endogenous metabolism of NO and H2S in plant cells and to deepen how the exogenous application of these compounds can contribute to crop resilience, particularly, against atmospheric stressors stimulating antioxidant systems.
KEAP1-Mutant Lung Cancers Weaken Anti-Tumor Immunity and Promote an M2-like Macrophage Phenotype
Authors: Occhiuto Christopher J. , Liby Karen T.
Published: March 2024

Considerable advances have been made in lung cancer therapies, but there is still an unmet clinical need to improve survival for lung cancer patients. Immunotherapies have improved survival, although only 20–30% of patients respond to these treatments. Interestingly, cancers with mutations in Kelch-like ECH-associated protein 1 (KEAP1), the negative regulator of the nuclear factor erythroid 2-related factor 2 (NRF2) transcription factor, are resistant to immune checkpoint inhibition and correlate with decreased lymphoid cell infiltration. NRF2 is known for promoting an anti-inflammatory phenotype when activated in immune cells, but the study of NRF2 activation in cancer cells has not been adequately assessed. The objective of this study was to determine how lung cancer cells with constitutive NRF2 activity interact with the immune microenvironment to promote cancer progression. To assess, we generated CRISPR-edited mouse lung cancer cell lines by knocking out the KEAP1 or NFE2L2 genes and utilized a publicly available single-cell dataset through the Gene Expression Omnibus to investigate tumor/immune cell interactions. We show here that KEAP1-mutant cancers promote immunosuppression of the tumor microenvironment. Our data suggest KEAP1 deletion is sufficient to alter the secretion of cytokines, increase expression of immune checkpoint markers on cancer cells, and alter recruitment and differential polarization of immunosuppressive macrophages that ultimately lead to T-cell suppression.
Disruption of Neuromuscular Junction Following Spinal Cord Injury and Motor Neuron Diseases
Authors: Nemeth Colin , Banik Naren L. , Haque Azizul
Published: March 2024

The neuromuscular junction (NMJ) is a crucial structure that connects the cholinergic motor neurons to the muscle fibers and allows for muscle contraction and movement. Despite the interruption of the supraspinal pathways that occurs in spinal cord injury (SCI), the NMJ, innervated by motor neurons below the injury site, has been found to remain intact. This highlights the importance of studying the NMJ in rodent models of various nervous system disorders, such as amyotrophic lateral sclerosis (ALS), Charcot–Marie–Tooth disease (CMT), spinal muscular atrophy (SMA), and spinal and bulbar muscular atrophy (SBMA). The NMJ is also involved in myasthenic disorders, such as myasthenia gravis (MG), and is vulnerable to neurotoxin damage. Thus, it is important to analyze the integrity of the NMJ in rodent models during the early stages of the disease, as this may allow for a better understanding of the condition and potential treatment options. The spinal cord also plays a crucial role in the functioning of the NMJ, as the junction relays information from the spinal cord to the muscle fibers, and the integrity of the NMJ could be disrupted by SCI. Therefore, it is vital to study SCI and muscle function when studying NMJ disorders. This review discusses the formation and function of the NMJ after SCI and potential interventions that may reverse or improve NMJ dysfunction, such as exercise, nutrition, and trophic factors.
Assessment of Blood and Semen Detection and DNA Collection from Swabs up to Three Months after Deposition on Five Different Cloth Materials
Authors: Medina-Paz Francisco , Kuba Brandon , Kryvorutsky Emily , Roca Gabriela , Zapico Sara C.
Published: March 2024

Body fluid identification plays a crucial role in criminal investigations. Because of their presence in many cases, blood and semen are the most relevant body fluids in forensic sciences. Based on antigen–antibody reactions binding unique proteins for each body fluid, serological assays represent one of the most rapid and highly specific tests for blood and semen. Currently, few studies have assessed the factors affecting body fluid identification by applying these assays. This work aimed to study the effect of different fabrics from clothes and time since deposition on identification through immunochromatographic tests for blood and semen, DNA isolation, and STR profiling from these samples. Body fluids were deposited on black- and white-dyed denim and cotton fabrics, and on leather. Afterward, blood and semen were sampled at 1 day, 30 days, and 90 days after deposition and identified by using the SERATEC® HemDirect Hemoglobin Test and the PSA Semiquant and SERATEC® BLOOD CS and SEMEN CS tests, respectively. Laboratory and crime scene tests presented similar performances for the detection of blood and semen stains on every tested fabric. No differences were found on band intensities between timepoints for all fabrics. It was possible to recover and identify blood and semen samples up to three months after deposition and to obtain full STR profiles from all the tested fabrics. Both body fluid STR profiles showed differences in their quality between 1 and 90 days after deposition for all fabrics except for black cotton for semen samples. Future research will expand the results, assessing body fluid identification on other substrates and under different environmental conditions.
Agroindustrial By-Products as a Source of Biostimulants Enhancing Responses to Abiotic Stress of Horticultural Crops
Authors: Zuzunaga-Rosas Javier , Boscaiu Monica , Vicente Oscar
Published: March 2024

Together with other abiotic stresses such as drought and high temperatures, salt stress is one of the most deleterious environmental factors affecting plant development and productivity, causing significant crop yield reductions. The progressive secondary salinisation of irrigated farmland is a problem as old as agriculture but is aggravated and accelerated in the current climate change scenario. Plant biostimulants, developed commercially during the last decade, are now recognised as innovative, sustainable agronomic tools for improving crop growth, yield, plant health and tolerance to abiotic stress factors such as water and soil salinity. Biostimulants are a disparate collection of biological extracts, natural and synthetic organic compounds or mixtures of compounds, inorganic molecules and microorganisms, defined by the positive effects of their application to crops. The growing interest in biostimulants is reflected in the increasing number of scientific reports published on this topic in recent years. However, the processes triggered by the biostimulants and, therefore, their mechanisms of action remain elusive and represent an exciting research field. In this review, we will mainly focus on one specific group of biostimulants, protein hydrolysates, generally produced from agricultural wastes and agroindustrial by-products—contributing, therefore, to more sustainable use of resources and circular economy—and primarily on the consequences of their application on the abiotic stress resistance of horticultural crops. We will summarise data in the scientific literature describing the biostimulants’ effects on basic, conserved mechanisms activated in response to elevated salinity and other abiotic stress conditions, such as the control of ion transport and ion homeostasis, the accumulation of osmolytes for osmotic adjustment, or the activation of enzymatic and non-enzymatic antioxidant systems to counteract the induced secondary oxidative stress. The collected information confirms the positive effects of biostimulants on crop tolerance to abiotic stress by enhancing morphological, physiological and biochemical responses, but also highlights that more work is needed to further establish the molecular mechanisms underlying biostimulants’ effects.
Consumption of Limosilactobacillus fermentum Inhibits Corneal Damage and Inflammation in Dry Eye Disease Mouse Model through Regulating the Gut Microbiome
Authors: Lee Kippeum , Gwon Hyeonjun , Shim Jae Jung , Kim Joo Yun , Lee Jae Hwan
Published: March 2024

The present study investigated the effect of orally administered Limosilactobacillus fermentum HY7302 (HY7302) on the relationship between ocular tissue and the microbiome in a corneal injury dry eye mouse model. Specifically, 0.1% benzalkonium chloride (BAC) was applied to the ocular surface for 14 days to induce corneal injury in male Balb/c mice. During the BAC treatment period, HY7302 (1 × 108 CFU/kg/day or 1 × 109 CFU/kg/day) or an omega-3 positive control (400 mg/kg/day) were administered orally (n = eight/group). To examine the signaling pathways affected by the HY7302 treatment, the in vitro effects of HY7302 on the tight junctions and the inflammatory response were investigated in the mouse colon epithelial cell line, CMT-93. BAC exposure decreased tear production, induced ocular inflammation and corneal epithelial detachment, and altered the gut microbiota. However, oral administration of HY7302 restored tear secretion and decreased corneal epithelial detachment in BAC-treated corneal injury mice. Further, HY7302 alleviated corneal inflammation via modulation of matrix metalloproteinase-9 (MMP-9) expression and affeted alterations in gut microbiota composition. These findings suggest that the gut–eye axis interaction between gut microbiota and corneal tissue affects disease severity in corneal injury, and that the alteration of the microbiota by HY7302 could improve eye health by regulating the inflammatory response.
Is Vesicular Therapy the Newcomer That Matters for the Medicine of Tomorrow?
Authors: Velot Émilie , Bianchi Arnaud
Published: March 2024

Extracellular vesicles (EVs) are membrane-enclosed particles released by cells into their extracellular environment [...]
Portuguese Neonatal Screening Program: A Cohort Study of 18 Years Using MS/MS
Authors: Gonçalves Maria Miguel , Marcão Ana , Sousa Carmen , Nogueira Célia , Fonseca Helena , Rocha Hugo , Vilarinho Laura
Published: March 2024

The Portuguese Neonatal Screening Program (PNSP) conducts nationwide screening for rare diseases, covering nearly 100% of neonates and screening for 28 disorders, including 24 inborn errors of metabolism (IEMs). The study’s purpose is to assess the epidemiology of the screened metabolic diseases and to evaluate the impact of second-tier testing (2TT) within the PNSP. From 2004 to 2022, 1,764,830 neonates underwent screening using tandem mass spectrometry (MS/MS) to analyze amino acids and acylcarnitines in dried blood spot samples. 2TT was applied when necessary. Neonates with profiles indicating an IEM were reported to a reference treatment center, and subsequent biochemical and molecular studies were conducted for diagnostic confirmation. Among the screened neonates, 677 patients of IEM were identified, yielding an estimated birth prevalence of 1:2607 neonates. The introduction of 2TT significantly reduced false positives for various disorders, and 59 maternal cases were also detected. This study underscores the transformative role of MS/MS in neonatal screening, emphasizing the positive impact of 2TT in enhancing sensitivity, specificity, and positive predictive value. Our data highlight the efficiency and robustness of neonatal screening for IEM in Portugal, contributing to early and life-changing diagnoses.
Odonata Assemblages in Urban Semi-Natural Wetlands
Authors: Vilenica Marina , Brigić Andreja , Štih Koren Ana , Koren Toni , Sertić Perić Mirela , Schmidt Bruno , Bužan Tomislava , Gottstein Sanja
Published: March 2024

As the human population in urban areas is continuously growing, urbanization is one of the greatest threats to biodiversity. To mitigate the negative effects, the inclusion of blue zones (aquatic habitats) in modern urban development practices is strongly recommended, as they could be beneficial for the local biodiversity conservation. Odonata are a flagship group and are widely used in freshwater conservation as ecological indicators of habitat integrity and health. However, our understanding of their ecological requirements in urban landscapes is not yet complete. Therefore, we analyzed the taxonomic and functional diversity of Odonata in a semi-natural wetland in the Croatian capital. This study was conducted in the summers of 2020 and 2023. Most taxonomic and functional assemblage metrics were comparable between the two main habitat types, anthropogenically disturbed and natural oxbow lakes. However, significant differences were found in relation to the time scale, where most metrics were lower in 2023, indicating the negative impact of extreme climate events (including droughts) that occurred in this region after 2020. With 19 species recorded, our results indicate that semi-natural urban wetlands, especially natural oxbow lakes, have great potential to function as good habitats for Odonata, where even some species of conservation concern were detected. When developing landscape management plans in urban areas, it is essential to consider the importance of habitat heterogeneity in terms of good structure of aquatic macrophytes (presence of submerged, emergent and floating vegetation), which would ensure the most suitable habitat conditions for local Odonata species.
Combinations of Lemongrass and Star Anise Essential Oils and Their Main Constituent: Synergistic Housefly Repellency and Safety against Non-Target Organisms
Authors: Soonwera Mayura , Sinthusiri Jirisuda , Passara Hataichanok , Moungthipmalai Tanapoom , Puwanard Cheepchanok , Sittichok Sirawut , Murata Kouhei
Published: March 2024

The present study evaluated the housefly repellency of single-component formulations and combinations of lemongrass and star anise essential oils (EOs) and their main constituents. The efficacies of the combinations were compared against those of single-component formulations and DEET. Safety bioassays of all formulations and DEET on non-target species—guppy, molly, dwarf honeybee, and stingless bee—were conducted. GC–MS analysis showed that the main constituent of lemongrass EO was geranial (46.83%) and that of star anise EO was trans-anethole (92.88%). All combinations were highly synergistic compared to single-component formulations, with an increased repellent value (IR) of 34.6 to 51.2%. The greatest synergistic effect was achieved by 1.0% lemongrass EO + 1.0% trans-anethole combination, with an IR of 51.2%. The strongest, 100% repellent rate at 6 h was achieved by 1.0% geranial + 1.0% trans-anethole. They were twice as effective as DEET and caused obvious damage to housefly antennae under microscopic observation. All single-component formulations and combinations were benign to the four tested non-target species. In contrast, DEET was highly toxic to them. The synergistic repellency and biosafety of these two combinations are compellingly strong support for developing them into an effective green repellent.
Extremely Rare Complications in Uniportal Spinal Endoscopy: A Systematic Review with Unique Case Analyses
Authors: Łątka Kajetan , Kołodziej Waldemar , Pawuś Dawid , Waligóra Marek , Trompeta Jacek , Klepinowski Tomasz , Lasowy Piotr , Tanaka Masato , Łabuz-Roszak Beata , Łątka Dariusz
Published: March 2024

Background: Endoscopic spine surgery represents a significant advancement in the minimally invasive treatment of spinal disorders, promising reduced surgical invasiveness while aiming to maintain or improve clinical outcomes. This study undertakes a comprehensive review of the literature on endoscopic spine surgery, with a particular focus on cataloging and analyzing the range of complications, from common postoperative issues to more severe, casuistic outcomes like dural tears and nerve damage. Methods: Our methodology encompassed a detailed review of meta-analyses, prospective randomized trials, cohort studies, and case reports to capture a broad spectrum of complications associated with endoscopic spine techniques. The emphasis was on identifying both the frequency and severity of these complications to understand better the procedural risks. Results: The findings suggest that endoscopic spine surgery generally exhibits a lower complication rate compared to traditional surgical approaches. Nonetheless, the identification of specific, rare complications peculiar to endoscopic methods underscores the critical need for surgeons’ advanced skills, continuous learning, and awareness of potential risks. Conclusions: Recognizing and preparing for the potential complications associated with the rapid adoption of endoscopic techniques is paramount to ensuring patient safety and improving surgical outcomes in minimally invasive spine surgery.
Reducing the Risk of Pre-Eclampsia in Women with Polycystic Ovary Syndrome Using a Combination of Pregnancy Screening, Lifestyle, and Medical Management Strategies
Authors: Parker Jim , O’Brien Claire , Yeoh Christabelle , Gersh Felice , Brennecke Shaun
Published: March 2024

Polycystic ovary syndrome (PCOS) is a multisystem disorder that presents with a variety of phenotypes involving metabolic, endocrine, reproductive, and psychological symptoms and signs. Women with PCOS are at increased risk of pregnancy complications including implantation failure, miscarriage, gestational diabetes, fetal growth restriction, preterm labor, and pre-eclampsia (PE). This may be attributed to the presence of specific susceptibility features associated with PCOS before and during pregnancy, such as chronic systemic inflammation, insulin resistance (IR), and hyperandrogenism, all of which have been associated with an increased risk of pregnancy complications. Many of the features of PCOS are reversible following lifestyle interventions such as diet and exercise, and pregnant women following a healthy lifestyle have been found to have a lower risk of complications, including PE. This narrative synthesis summarizes the evidence investigating the risk of PE and the role of nutritional factors in women with PCOS. The findings suggest that the beneficial aspects of lifestyle management of PCOS, as recommended in the evidence-based international guidelines, extend to improved pregnancy outcomes. Identifying high-risk women with PCOS will allow targeted interventions, early-pregnancy screening, and increased surveillance for PE. Women with PCOS should be included in risk assessment algorithms for PE.
A Long-Term Comparative Analysis of Endovascular Coiling and Clipping for Ruptured Cerebral Aneurysms: An Individual Patient-Level Meta-Analysis Assessing Rerupture Rates
Authors: Wach Johannes , Vychopen Martin , Güresir Agi , Guranda Alexandru , Nestler Ulf , Güresir Erdem
Published: March 2024

Background: Although the initial functional outcome findings of the International Subarachnoid Aneurysm Trial (ISAT) study favored coiling at one year after aneurysmal subarachnoid hemorrhage (aSAH), concerns arose regarding limited long-term rerupture data. This meta-analysis is the first to analyze longitudinal individual patient data (IPD) of target aneurysm rerupture in terms of treatment modality. Methods: The present meta-analysis included studies that compared clipping with coiling of ruptured aneurysms regarding long-term rerupture. Rerupture rates’ individual patient data (IPD) were extracted from published Kaplan–Meier curves utilizing the R package IPDfromKM in R Version 4.3.1. Results: A total of 3153 patients from two studies were included. The clipping arm included 1755 patients, whereas the coiling arm included 1398 patients. Median reconstructed follow-up was 6.1 years (IQR = 0.5–11.7). The rerupture rates in the clipping arm and the coiling arm were 0.5% and 1.5%, respectively (p = 0.002). Kaplan–Meier chart analysis of the 3153 patients revealed a shortened time to rerupture in the coiling arm (log-rank test: p = 0.01). The hazard ratio (HR) for coiling compared with clipping regarding rerupture was 3.62 (95% CI:1.21–10.86, p = 0.02). Conclusion: Target aneurysm rerupture was rare beyond the initial year. Pooled long-term IPD from the 3153 patients revealed that reruptures of target aneurysms are more common after coiling and might be considered in the pretherapeutic decision-making process for aSAH.
Enhancing Diagnostic Precision: Evaluation of Preprocessing Filters in Simple Diffusion Kurtosis Imaging for Head and Neck Tumors
Authors: Nakamitsu Yuki , Kuroda Masahiro , Shimizu Yudai , Kuroda Kazuhiro , Yoshimura Yuuki , Yoshida Suzuka , Nakamura Yoshihide , Fukumura Yuka , Kamizaki Ryo , Al-Hammad Wlla E. , Oita Masataka , Tanabe Yoshinori , Sugimoto Kohei , Sugianto Irfan , Barham Majd , Tekiki Nouha , Asaumi Junichi
Published: March 2024

Background: Our initial clinical study using simple diffusion kurtosis imaging (SDI), which simultaneously produces a diffusion kurtosis image (DKI) and an apparent diffusion coefficient map, confirmed the usefulness of SDI for tumor diagnosis. However, the obtained DKI had noticeable variability in the mean kurtosis (MK) values, which is inherent to SDI. We aimed to improve this variability in SDI by preprocessing with three different filters (Gaussian [G], median [M], and nonlocal mean) of the diffusion-weighted images used for SDI. Methods: The usefulness of filter parameters for diagnosis was examined in basic and clinical studies involving 13 patients with head and neck tumors. Results: The filter parameters, which did not change the median MK value, but reduced the variability and significantly homogenized the MK values in tumor and normal tissues in both basic and clinical studies, were identified. In the receiver operating characteristic curve analysis for distinguishing tumors from normal tissues using MK values, the area under curve values significantly improved from 0.627 without filters to 0.641 with G (σ = 0.5) and 0.638 with M (radius = 0.5). Conclusions: Thus, image pretreatment with G and M for SDI was shown to be useful for improving tumor diagnosis in clinical practice.
Feasibility of Fluid Responsiveness Assessment in Patients at Risk for Increased Intracranial Pressure
Authors: Zivkovic Aleksandar R. , Kjaev Aleko , Schönenberger Silvia , Krieg Sandro M. , Weigand Markus A. , Neumann Jan-Oliver
Published: March 2024

Background: Effective fluid management is important for patients at risk of increased intracranial pressure (ICP). Maintaining constant cerebral perfusion represents a challenge, as both hypovolemia and fluid overload can severely impact patient outcomes. Fluid responsiveness tests, commonly used in critical care settings, are often deemed potentially hazardous for these patients due to the risk of disrupting cerebral perfusion. Methods: This single-center, prospective, clinical observational study enrolled 40 patients at risk for increased ICP, including those with acute brain injury. Informed consent was obtained from each participant or their legal guardians before inclusion. The study focused on the dynamics of ICP and cerebral perfusion pressure (CPP) changes during the Passive Leg Raise Test (PLRT) and the End-Expiratory Occlusion Test (EEOT). Results: The results demonstrated that PLRT and EEOT caused minor and transient increases in ICP, while consistently maintaining stable CPP. EEOT induced significantly lower ICP elevations, making it particularly suitable for use in high-risk situations. Conclusions: PLRT and EEOT can be considered feasible and safe for assessing fluid responsiveness in patients at risk for increased ICP. Notably, EEOT stands out as a preferred method for high-risk patients, offering a dependable strategy for fluid management without compromising cerebral hemodynamics.
The Impact of Regional Anesthesia in Masking Acute Compartment Syndrome after Limb Trauma
Authors: Hilber Nicole , Dodi Anna , Blumenthal Stephan , Bruppacher Heinz , Borgeat Alain , Aguirre José
Published: March 2024

Regional anesthesia has shown to be successful in controlling major pain in trauma patients. However, the possibility of masking acute compartment syndrome (ACS) after peripheral nerve blocks for limb injuries is still controversially discussed. Therefore, we aimed to summarize the current literature regarding this topic to shed light on the impact of peripheral regional anesthesia on the diagnosis of ACS in trauma patients. We searched Pubmed, Google Scholar and the Cochrane Library for literature following the PRISMA (preferred reporting items for systematic reviews and meta-analyses) guidelines. The analysis of these reports was included in the context of the current literature concerning this topic. We found no (randomized) studies, and only six case reports dealing with the impact of peripheral nerve blocks and ACS in patients after a limb trauma met our criteria and were included in our review. Only one reported a delay in the diagnosis of ACS. In most of the cases (5 of 6), the breakthrough pain, despite the nerve block, proved to be a good indicator of a developing ACS. However, despite some narrative articles about the topic including some recommendations about the possibly safe use of regional anesthesia techniques for limb trauma, there is still no international consensus and only one national guideline focusing on the possibly safe use of peripheral nerve blocks in trauma patients at risk of ACS. After reviewing the respective literature, we consider that intra-articular analgesia, sensory blocks, fascial plane blocks and low-concentration continuous peripheral nerve blocks are effective for analgesia and a low-risk analgesia tool for trauma and postsurgical patients at risk of ACS due to the fact that they do not lead to a dense block. Finally, we summarized suggestions based on the results of the literature for the different regional anesthesia modalities in these patients in a table to facilitate the use of these techniques.
A Simplified Gm − C Filter Technique for Reference Spur Reduction in Phase-Locked Loop
Authors: Chary P. Purushothama , Shaik Peerla Rizwan , Dutta Ashudeb
Published: March 2024

This paper presents a wideband approach for L5 and S-band integer-N phase-locked loop (PLL) targeting Indian Regional Navigation Satellite System (IRNSS) applications. A reference spur reduction technique using a Gm−C filter is proposed. The reference spur is improved by 7 dB when compared with one without any Gm−C filter. The wideband integer-N PLL is designed and fabricated in UMC 65-nm CMOS process. The Gm−C filter block consumes 200 μA current. The wideband voltage-controlled oscillator (VCO) oscillates from 1.6 GHz to 3.2 GHz having a tuning range (TR) of 40%, achieving a best and worst phase noise of ≈−122 dBc/Hz and ≈−116 dBc/Hz at a 1 MHz offset, respectively.
A Weakly Nonlinear System for Waves and Sheared Currents over Variable Bathymetry
Authors: Touboul Julien , Morales-Marquez Veronica , Belibassakis Kostas
Published: March 2024

The wave–current–seabed interaction problem is studied by using a coupled-mode system developed for modeling wave scattering by non-homogeneous, sheared currents in variable bathymetry regions. The model is based on a modal series expansion of wave velocity based on vertical eigenfunctions, dependent on local depth and flow parameters, including propagating and evanescent modes. The latter representation is able to accurately satisfy the wave flow continuity condition and the no-entrance boundary condition on the sloping parts of the seabed. A new derivation of a simplified nonlinear system is introduced using decomposition to a mean flow and a perturbative wave field. To force the system to consider incoming waves at the inlet, boundary knowledge of periodic, travelling nonlinear water waves over a flat bottom is required. For this purpose, specific solutions are derived using the semi-analytical method based on the stream function formulation, for cases of water waves propagating above linearly and exponentially sheared currents. Results obtained by the application of the CMS concerning the propagation of waves and currents—in particular, examples characterized by depth inhomogeneities—are presented and discussed, illustrating the applicability and performance of the method.
Net Transport Patterns of Surficial Marine Sediments in the North Aegean Sea, Greece
Authors: Vakalas Ioannis , Zananiri Irene
Published: March 2024

The spatial distribution of sediments on the seafloor reflects the various dynamic processes involved in the marine realm. To analyze sediment transport patterns in the North Aegean Sea, 323 surficial samples were obtained and studied. The granulometry data revealed a diverse range of grain sizes of surficial sediments, ranging from purely sandy to clay. The predominant size classes were silt and muddy sand, followed by sandy silt and mud. However, there were very few samples that fell within the clay classes. The sorting coefficient ranged from 0.21 to 5.48, while skewness ranged from −1.09 to 1.29. The sediment transport patterns were analyzed based on the grain-size parameters (mean, sorting, and skewness). The results showed the variability of flow parameters involved in sediment distribution. River influx and longshore drift near the shoreline are the most significant factors affecting sediment transport. At the open sea, sediment distribution is mainly controlled by general water circulation patterns, especially by the outflow of low-salinity waters from the Black Sea through the Dardanelles and the Marmara Sea. The heterogeneity of sediment textural parameters across the study area suggests that seafloor sediments are further reworked in areas where water masses are highly energetic. It can be concluded that open sea water circulation controls sediment distribution patterns at the open shelf, while close to the coast, river discharge plays a key role.
An Adaptive Multimodal Data Vessel Trajectory Prediction Model Based on a Satellite Automatic Identification System and Environmental Data
Authors: Xiao Ye , Hu Yupeng , Liu Jizhao , Xiao Yi , Liu Qianzhen
Published: March 2024

Ship trajectory prediction is essential for ensuring safe route planning and to have advanced warning of the dangers at sea. With the development of deep learning, most of the current research has explored advanced prediction methods based on historical spatio-temporal Automatic Identification System (AIS) data. However, environmental factors such as sea wind and visibility also affect ship navigation in real-world maritime shipping. Therefore, developing reliable models utilizing multimodal data, such as AIS and environmental data, is challenging. In this research, we design an adaptive multimodal vessel trajectory data prediction model (termed AMD) based on satellite AIS and environmental data. The AMD model mainly consists of an AIS-based extraction network, an environmental-based extraction network, and a fusion block. In particular, this work considers multimodal data such as historical spatio-temporal information and environmental factors. Time stamps and distances are correlated with AIS and environmental data, and a multilayer perceptron and gated recurrent unit networks are used to design multimodal feature extraction networks. Finally, the fusion block realizes the fusion output of multimodal features to improve the reliability of the AMD model. Several quantitative and qualitative experiments are conducted using real-world AIS and multimodal environmental datasets. Numerous experimental results prove that prediction performance using multimodal data can ensure satisfactory accuracy and reliability while exhibiting a positive impact on improving maritime transport services.
The Effect of Green Extraction Technologies on the Chemical Composition of Medicinal Chaga Mushroom Extracts
Authors: Lazić Vesna , Klaus Anita , Kozarski Maja , Doroški Ana , Tosti Tomislav , Simić Siniša , Vunduk Jovana
Published: March 2024

The mushroom industry should implement green extraction technologies; however, there is not enough information on the differences between these techniques expressed as the chemical composition of the resulting extract. In this study, selected types of green extraction techniques (GETs) were used on Chaga (Inonotus obliquus) (Fr.) Pilát from Serbia (IS) and Mongolia (IM) to examine the differences that would enable the composition-based technology choices in the mushroom supplement industry. Subcritical water extraction (SWE), microwave-assisted (MW) extraction, and ultrasonic-assisted extraction (VAE) were used to prepare the extracts. SWE was performed at two different temperatures (120 and 200 °C), while 96% ethanol, 50% ethanol, and water were used for MW and VAE. The yield, the content of total phenols, total proteins, and carbohydrates, qualitative and quantitative analysis of phenolic compounds, carbohydrates, including α- and β- and total glucans, and fatty acids, were determined in the obtained extracts. SWE resulted in a significantly higher yield, total polysaccharide, and glucan content than any other technique. Glucose was the most dominant monosaccharide in the SWE samples, especially those extracted at 200 °C. The MW 50% EtOH extracts showed the highest yield of total phenols. Among the tested phenolic compounds, chlorogenic acid was the most dominant. SWE can be recommended as the most efficient method for extracting commercially important compounds, especially glucans and phenols.
Assessment of a New Medical Device (PirifixTM) for Positioning and Maintaining the Upper Dental Arch during Le Fort I Osteotomy
Authors: Serree Pierre-Etienne , Bertin Eugénie , Coussens Camille , Brumpt Eleonore , Devoti Jean-François , Louvrier Aurélien
Published: March 2024

Introduction: Several medical devices (MDs) are used to assist surgeons in positioning the upper dental arch (UDA) during Le Fort I osteotomies (LFIOs). Some only allow holding, others only positioning. This study aimed to assess the accuracy of a new MD (PirifixTM) coupling these two functions during LFIO on 3D-printed models. Materials and Methods: DICOM data were selected from patients who underwent surgical planning for LFIO between 27 July 2020 and 1 December 2022. Their anatomy was reproduced after segmentation, planning, and stereolithography in two models. Each model was assigned to one of two surgical groups: the control group (positioning by occlusal splint) and the PirifixTM group. Each patient’s model was planned with the objective of horizontalizing and recentering the UDA. After positioning, models were digitalized using Einscan Pro 2X and compared to the planned model with CloudCompare. The statistical analysis was performed using the Wilcoxon Mann–Whitney test. The result was considered significant if the p-value was less than 0.05. Results: Twenty-one patients were selected. Forty-two anatomical models were 3D-printed. The mean difference compared to the planned and corrected positions was 0.69 mm for the control group and 0.84 mm for the PirifixTM group (p = 0.036). Conclusion: PirifixTM may be a new alternative to available MDs. Further investigations are needed to describe the relationship between the device and facial soft tissues.
The Current and Expected Pricing Markup as Derived from the Capital Asset Pricing Model and Tobin’s Q and Applied to the UK’s FTSE 100
Authors: Hackworth Paul
Published: March 2024

Price markups and firms’ Tobin’s Q ratios are widely believed to have been increasing in the past several decades. Various models for the calculation of price markups have been developed, each relying on the historically held definition of the ratio of price to marginal cost; however, all of these have methodological drawbacks, and some of the results they have produced have been poorly reflective of the near past wider macroeconomic experience. This paper defines a new approach for the definition and measurement of markup pricing, and it also avoids some of the issues surrounding the marginal cost approaches by using the measure of economic rent and the capital asset pricing model. The results show limited markup pricing for the UK’s FTSE 100 companies (2018–2023), but that certain real estate, technology/media and financial services/equity investment firms have enjoyed higher price markup levels. An analysis of the business models of these firms is used to qualitatively propose explanations for such markups. This work offers formal proof that that the expected price markup is equal to Tobin’s Q and finds that the empiric market level of markup is near equivalent to the market Tobin’s Q; the differences between the markup and Tobin’s Q at the level of the firm are equally assessed. This work challenges the general consensus that price markups are above one and have been increasing; it may also aid policy makers with respect to taxation policy and regulatory measures, as well as the financial management of firms in decisions concerning capital deployment and portfolio management. The method merits expansion to wider data sets, as well as to those from outside of the UK.
An Experimental Investigation into the Scope Assignment of Japanese and Chinese Quantifier-Negation Sentences
Authors: Chen Yunchuan
Published: March 2024

Quantifier-Negation sentences such as all teachers did not use Sandy’s car are known to allow an inverse scope interpretation in English. However, there is a lack of experimental evidence to determine whether this interpretation is allowed in equivalent sentences in Japanese and Chinese. To address this issue, this study conducted a sentence–picture matching truth value judgment experiment in both Japanese and Chinese. The data suggested that Japanese Quantifier-Negation sentences do allow inverse scope readings, which suggests that the subject may be interpreted within the scope of negation. In contrast, Chinese Quantifier-Negation sentences prohibit inverse scope readings, which is in accordance with the strong scope rigidity consistently observed in this language. This paper also discussed how to develop a valid experiment for investigating scope ambiguities.