bluuebunny/crossref_metadata_2025 huggingface dataset:
Sources of Onboard Fumes and Smoke Reported by U.S. Airlines
Authors: Anderson Judith
Published: April 2021

This paper describes the relative frequency of reports of oil and hydraulic fluid fumes in the ventilation supply air (“fume events”) compared to other types of fumes and smoke reported by U.S. airlines over 10 years. The author reviewed and categorized 12,417 fume/smoke reports submitted to the aviation regulator to comply with the primary maintenance reporting regulation (14 CFR § 121.703) from 2002–2011. The most commonly documented category of onboard fumes/smoke was electrical (37%). Combining the categories of “bleed-sourced”, “oil”, and “hydraulic fluid” created the second most prevalent category (26%). The remaining sources of onboard fumes/smoke are also reported. To put the data in context, the fume event reporting regulations are described, along with examples of ways in which certain events are underreported. These data were reported by U.S. airlines, but aviation regulations are harmonized globally, so the data likely also reflect onboard sources of fumes and smoke reported in other countries with equivalent aviation systems. The data provide insight into the relative frequency of the types of reported fumes and smoke on aircraft, which should drive design, operational, and maintenance actions to mitigate onboard exposure. The data also provide insight into how to improve current fume event reporting rules.
Virulence Determinants and Antimicrobial Profiles of Pasteurella multocida Isolated from Cattle and Humans in Egypt
Authors: Elsayed Mohamed Sabry Abd Elraheam , Eldsouky Samah Mahmoud , Roshdy Tamer , Said Lamia , Thabet Nahed , Allam Tamer , Mohammed A. B. Abeer , Nasr Ghada M. , Basiouny Mohamed S. M. , Akl Behairy A. , Nader Maha M. , Hasan Al Shaimaa , Salah Ahmed
Published: April 2021

Pasteurella multocida is a Gram-negative bacterium that causes drastic infections in cattle and humans. In this study, 55 isolates were recovered from 115 nasal swabs from apparently healthy and diseased cattle and humans in Minufiya and Qalyubia, Egypt. These isolates were confirmed by kmt1 existence, and molecular classification of the capsular types showed that types B, D, and E represented 23/55 (41.8%), 21/55 (38.1%), and 11/55 (20.0%), respectively. The isolates were screened for five virulence genes with hgbA, hgbB, and ptfA detected in 28/55 (50.9%), 30/55 (54.5%), and 25/55 (45.5%), respectively. We detected 17 capsular and virulence gene combinations with a discriminatory power (DI) of 0.9286; the most prevalent profiles were dcbF type D and dcbF type D, hgbA, hgbB, and ptfA, which represented 8/55 (14.5%) each. These strains exhibited high ranges of multiple antimicrobial resistance indices; the lowest resistances were against chloramphenicol, ciprofloxacin, amoxicillin/clavulanic acid, and levofloxacin. The macrolide–lincosamide–streptogramin B methylase gene erm(Q), with erm(42) encoding MLSB monomethyltransferase, mph(E) encoding a macrolide efflux pump, and msr(E) encoding macrolide-inactivating phosphotransferase were present. The class 1 and 2 integrons and extended-spectrum β-lactamase genes intl1, intl2, blaCTX-M, blaCTX-M-1, and blaTEM were detected. It is obvious to state that co-occurrence of resistance genes resulted in multiple drug-resistant phenotypes. The identified isolates were virulent, genetically diverse, and resistant to antimicrobials, highlighting the potential risk to livestock and humans.
Long-Term Use of Oral Hygiene Products Containing Stannous and Fluoride Ions: Effect on Viable Salivary Bacteria
Authors: Kruse Anne Brigitte , Schlueter Nadine , Kortmann Viktoria Konstanze , Frese Cornelia , Anderson Annette , Wittmer Annette , Hellwig Elmar , Vach Kirstin , Al-Ahmad Ali
Published: April 2021

The aim of this randomized, controlled clinical trial was to isolate and identify viable microorganisms in the saliva of study participants that continuously used a stannous and fluoride ion (F/Sn)-containing toothpaste and mouth rinse over a period of three years in comparison to a control group that used stannous ion free preparations (noF/Sn) over the same time period. Each group (F/Sn and noF/Sn) included 16 participants that used the respective oral hygiene products over a 36-month period. Stimulated saliva samples were collected at baseline (T0) and after 36 months (T1) from all participants for microbiological examination. The microbial composition of the samples was analyzed using culture technique, matrix-assisted laser desorption ionization–time of flight (MALDI–TOF) mass spectrometry, and 16S rDNA Polymerase Chain Reaction (PCR). There were only minor differences between both groups when comparing the absolute values of viable microbiota and bacterial composition. The treatment with F/Sn led to a slight decrease in disease-associated and a slight increase in health-associated bacteria. It was shown that the use of stannous ions had no negative effects on physiological oral microbiota even after prolonged use. In fact, a stabilizing effect of the oral hygiene products containing stannous ions on the health-associated oral microbiota could be expected.
Can Prophylactic High Flow of Humidified and Warmed Filtered Air Improve Survival from Bacterial Pneumonia and SARS-CoV-2 in Elderly Individuals? The Role of Surfactant Protein A
Authors: Abbasi Ata , Phelps David S. , Ravi Radhika , Floros Joanna
Published: April 2021

In this opinion article, we discuss a serendipitous observation we made in a study investigating survival in aged mice after bacterial infection. This observation involved a non-invasive ventilation approach that led to variable and higher survival in male and female mice with different genetic backgrounds for the innate immune molecule, surfactant protein A (SP-A). We suggest that employing the best ventilatory modality, whether that be HFNC or another method, may augment the role of other factors such as SP-A genetics and sex in a personalized approach, and may ultimately improve the outcome.
The Effects of Matriptase Inhibition on the Inflammatory and Redox Homeostasis of Chicken Hepatic Cell Culture Models
Authors: Barna Réka Fanni , Mackei Máté , Pászti-Gere Erzsébet , Neogrády Zsuzsanna , Jerzsele Ákos , Mátis Gábor
Published: April 2021

The function of the transmembrane serine protease matriptase is well described in mammals, but it has not been elucidated in avian species yet. Hence, the aim of the present study was to assess the effects of the 3-amidinophenylalanine (3-AphA)-type matriptase inhibitors MI432 and MI460 on the inflammatory and oxidative state of chicken primary hepatocyte mono-cultures and hepatocyte–nonparenchymal cell co-cultures, the latter serving as a proper model of hepatic inflammation in birds. Cell cultures were exposed to MI432 and MI460 for 4 and 24 h at 10, 25, and 50 µM concentrations, and thereafter the cellular metabolic activity, extracellular interleukin (IL-)6, IL-8, H2O2 and malondialdehyde concentrations were monitored. Both inhibitors caused a transient moderate reduction in the metabolic activity following 4 h exposure, which was restored after 24 h, reflecting the fast hepatic adaptation potential to matriptase inhibitor administration. Furthermore, MI432 triggered an intense elevation in the cellular proinflammatory IL-6 and IL-8 production after both incubation times in all concentrations, which was not coupled to enhanced oxidative stress and lipid peroxidation based on unchanged H2O2 production, malondialdehyde levels and glutathione peroxidase activity. These data suggest that physiological matriptase activities might have a key function in retaining the metabolic and inflammatory homeostasis of the liver in chicken, without being a major modulator of the hepatocellular redox state.
Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy
Authors: Kim Kyeong-Seok , Lee Jin-Sol , Park Jae-Hyeon , Lee Eun-Young , Moon Jong-Seok , Lee Sang-Kyu , Lee Jong-Sil , Kim Jung-Hwan , Kim Hyung-Sik
Published: April 2021

Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus. After development of DN, patients will progress to end-stage renal disease, which is associated with high morbidity and mortality. Here, we developed early-stage diagnostic biomarkers to detect DN as a strategy for DN intervention. For the DN model, Zucker diabetic fatty rats were used for DN phenotyping. The results revealed that DN rats showed significantly increased blood glucose, blood urea nitrogen (BUN), and serum creatinine levels, accompanied by severe kidney injury, fibrosis and microstructural changes. In addition, DN rats showed significantly increased urinary excretion of kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL). Transcriptome analysis revealed that new DN biomarkers, such as complementary component 4b (C4b), complementary factor D (CFD), C-X-C motif chemokine receptor 6 (CXCR6), and leukemia inhibitory factor (LIF) were identified. Furthermore, they were found in the urine of patients with DN. Since these biomarkers were detected in the urine and kidney of DN rats and urine of diabetic patients, the selected markers could be used as early diagnosis biomarkers for chronic diabetic nephropathy.
Non-Invasive Monitoring of the Spatio-Temporal Dynamics of Vocalizations among Songbirds in a Semi Free-Flight Environment Using Robot Audition Techniques
Authors: Sumitani Shinji , Suzuki Reiji , Arita Takaya , Nakadai Kazuhiro , Okuno Hiroshi G.
Published: April 2021

To understand the social interactions among songbirds, extracting the timing, position, and acoustic properties of their vocalizations is essential. We propose a framework for automatic and fine-scale extraction of spatial-spectral-temporal patterns of bird vocalizations in a densely populated environment. For this purpose, we used robot audition techniques to integrate information (i.e., the timing, direction of arrival, and separated sound of localized sources) from multiple microphone arrays (array of arrays) deployed in an environment, which is non-invasive. As a proof of concept of this framework, we examined the ability of the method to extract active vocalizations of multiple Zebra Finches in an outdoor mesh tent as a realistic situation in which they could fly and vocalize freely. We found that localization results of vocalizations reflected the arrangements of landmark spots in the environment such as nests or perches and some vocalizations were localized at non-landmark positions. We also classified their vocalizations as either songs or calls by using a simple method based on the tempo and length of the separated sounds, as an example of the use of the information obtained from the framework. Our proposed approach has great potential to understand their social interactions and the semantics or functions of their vocalizations considering the spatial relationships, although detailed understanding of the interaction would require analysis of more long-term recordings.
Absolute Configuration of In Situ Crystallized (+)-γ-Decalactone
Authors: Patzer Michael , Nöthling Nils , Goddard Richard , Lehmann Christian W.
Published: April 2021

Knowledge about the absolute configuration of small bioactive organic molecules is essential in pharmaceutical research because enantiomers can exhibit considerably different effects on living organisms. X-ray crystallography enables chemists to determine the absolute configuration of an enantiopure compound due to anomalous dispersion. Here, we present the determination of the absolute configuration of the flavoring agent (+)-γ-decalactone, which is liquid under ambient conditions. Single crystals were grown from the liquid in a glass capillary by in situ cryo-crystallization. Diffraction data collection was performed using Cu-Kα radiation. The absolute configuration was confirmed. The molecule consists of a linear aliphatic non-polar backbone and a polar lactone head. In the solid state, layers of polar and non-polar sections of the molecule alternating along the c-axis of the unit cell are observed. In favorable cases, this method of absolute configuration determination of pure liquid (bioactive) agents or liquid products from asymmetric catalysis is a convenient alternative to conventional methods of absolute structure determination, such as optical rotatory dispersion, vibrational circular dichroism, ultraviolet-visible spectroscopy, use of chiral shift reagents in proton NMR and Coulomb explosion imaging.
Risperidone or Aripiprazole Can Resolve Autism Core Signs and Symptoms in Young Children: Case Study
Authors: Alsayouf Hamza A. , Talo Haitham , Biddappa Marisa L. , De Los Reyes Emily
Published: April 2021

Risperidone and aripiprazole are approved by the USA Food and Drug Administration for the treatment of irritability and aggression in children from the ages of 5 and 6 years, respectively. However, there are no approved medications for the treatment of autism spectrum disorder (ASD) core signs and symptoms. Nevertheless, early intervention is recognized as key to improving long-term outcomes. This retrospective case study included 10 children (mean age, 2 years 10 months) with ASD who presented with persistent irritability and aggression before 4 years of age that was unresponsive to behavioral interventions and sufficiently severe to consider pharmacological intervention with risperidone or aripiprazole combined with standard supportive therapies. Besides ameliorating comorbid behaviors, improvement was observed in ASD core signs and symptoms for all patients, with minimal-to-no symptoms observed in 60% of patients according to the Childhood Autism Rating Scale 2-Standard Test and Clinical Global Impression scales. Excessive weight gain in two patients was the only adverse effect observed that required intervention. This is the first study to suggest that ASD can potentially be treated in very young children (<4 years). Clinical trials are urgently required to validate these findings among this pediatric population.
Parent and Peer Emotion Responsivity Styles: An Extension of Gottman’s Emotion Socialization Parenting Typologies
Authors: Jespersen Jens E. , Hardy Nathan R. , Morris Amanda Sheffield
Published: April 2021

This theoretical paper introduces six emotion socialization typologies that can be used for designating emotion responsivity styles of parents and peers of children in middle childhood, referred to as Parent and Peer Emotion Responsivity Styles (PPERS). This typology draws on theoretical foundations of meta-emotion and emotion socialization. These typologies are compliment with and extend Gottman’s emotion-based parenting styles, as they are organized generally by whether the response is more positive or more negative and whether the response is more emotionally constructive or destructive, but extend the four styles to include whether the parent or peer targets the emotion directly when responding to a child’s emotions, or whether they target the emotion-related behavior. On the positive end, there is the Emotion Constructive style, which targets the child’s emotions directly. The other two positive styles include Emotion Responsive and Emotion Acceptive, which target the child’s emotional behaviors with higher or lower levels of activity. On the negative side, there is the Emotion Destructive style which is employed to target the emotion itself, while the Emotion Punitive and Emotion Dismissive styles target the child’s emotion-related behavior with varying levels of activity. Implications for the development and study of these theoretical typologies are discussed.
Lessons in Stories: Why Narrative Medicine Has a Role in Pediatric Palliative Care Training
Authors: Lanocha Natalie
Published: April 2021

Narrative medicine is introduced and explored as a potential tool for developing competency in medical training, including reduction of burnout, sustaining empathy, and allowing for reflective practice. Developing cultural humility, communication skills, ethics, community building, and advocacy are also reviewed as domains that may be bolstered by training in narrative. Applications specific to pediatric palliative care are suggested, along with avenues for further research.
Overview of the Enablers and Barriers for a Wider Deployment of CSP Tower Technology in Europe
Authors: Aprà Fabio Maria , Smit Sander , Sterling Raymond , Loureiro Tatiana
Published: April 2021

For years, concentrated solar power (CSP) has been considered an emerging technology that could disrupt the energy production sector. The possibility to store the electricity generated during the sunny operating hours in the form of heat enhances energy dispatchability and gives CSP a unique value proposition that conventional renewable energies cannot provide cost-efficiently since it requires the integration of costly large-scale battery systems. CSP is a cleaner technology compared to photovoltaics, but photovoltaics currently has lower overall capital costs, making it more attractive to investors and stakeholders who want to spend less money upfront. This is one of the main reasons why CSP has never really led either the electricity market or the heating one, even if its combined generation capability (heat and electricity) is globally recognized as a great advantage for a renewable technology. In this study, we analyze the reasons why CSP is not as widespread as it could be; at the same time, we look at the opportunities and the enablers for a further deployment of this technology, focusing on the European region.
Meal and Sleep Timing before and during the COVID-19 Pandemic: A Cross-Sectional Anonymous Survey Study from Sweden
Authors: Benedict Christian , Brandão Luiz Eduardo Mateus , Merikanto Ilona , Partinen Markku , Bjorvatn Bjørn , Cedernaes Jonathan
Published: April 2021

The COVID-19 pandemic and related restrictions, such as stay-at-home-orders, have significantly altered daily routines and lifestyles. Given their importance for metabolic health, we herein compared sleep and meal timing parameters during vs. before the COVID-19 pandemic based on subjective recall, in an anonymous Swedish survey. Among 191 adults (mean age: 47 years; 77.5% females), we show that social jetlag, i.e., the mismatch in sleep midpoint between work and free days, was reduced by about 17 min during the pandemic compared with the pre-pandemic state (p < 0.001). Concomitantly, respondents’ sleep midpoint was shifted toward morning hours during workdays (p < 0.001). A later daily eating midpoint accompanied the shift in sleep timing (p = 0.001). This effect was mainly driven by a later scheduled first meal (p < 0.001). No difference in the timing of the day’s last meal was found (p = 0.814). Although our survey was limited in terms of sample size and by being cross-sectional, our results suggest that the delay in sleep timing due to the COVID-19 pandemic was accompanied by a corresponding shift in the timing of early but not late meals.
Corrosion Performance of Electrodeposited Zinc and Zinc-Alloy Coatings in Marine Environment
Authors: Maniam Kranthi Kumar , Paul Shiladitya
Published: April 2021

Electrodeposited zinc and zinc-alloy coatings have been extensively used in a wide variety of applications such as transport, automotive, marine, and aerospace owing to their good corrosion resistance and the potential to be economically competitive. As a consequence, these coatings have become the industry choice for many applications to protect carbon and low alloy steels against degradation upon their exposure in different corrosive environments such as industrial, marine, coastal, etc. Significant works on the electrodeposition of Zn, Zn-alloys and their composites from conventional chloride, sulfate, aqueous and non-aqueous electrolyte media have been progressed over the past decade. This paper provides a review covering the corrosion performance of the electrodeposited Zn, Zn-alloy and composite with different coating properties that have been developed over the past decade employing low-toxic aqueous and halide-free non-aqueous electrolyte media. The influence of additives, nano-particle addition to the electrolyte media on the morphology, texture in relation to the corrosion performance of coatings with additional functionalities are reviewed in detail. In addition, the review covers the recent developments along with cost considerations and the future scope of Zn and Zn-alloy coatings.
A Rapid and Sensitive Method for the Determination of Cannabidiol in Cosmetic Products by Liquid Chromatography–Tandem Mass Spectrometry
Authors: Schettino Lorenza , Prieto Marta , Benedé Juan L. , Chisvert Alberto , Salvador Amparo
Published: April 2021

Cannabidiol is a phytocannabinoid with proven pharmacological properties that is also used in the cosmetic industry for its sebostatic and antioxidant activities, being considered a new anti-aging ally. An analytical method is proposed for the determination of CBD in cosmetic products by liquid chromatography with tandem mass spectrometry, after leaching the CBD from the cosmetic matrix with ethanol. Low instrumental limits of detection (0.22 ng mL−1) and quantification (0.74 ng mL−1) allow the determination of CBD at trace levels without needing preconcentration, whereas the wide linearity of the method allows the determination of CBD in more concentrated samples without high dilution. The method was successfully applied to the analysis of six cosmetic products and a raw material. The proposed method is suitable for the quality control of cosmetic products containing CBD, being able to quickly and easily determine this compound, ensuring that its concentration in the finished product is the desired one.
Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy
Authors: Zhang Chen , Welsher Kevin
Published: April 2021

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.
Optimization Modeling of Irreversible Carnot Engine from the Perspective of Combining Finite Speed and Finite Time Analysis
Authors: Costea Monica , Petrescu Stoian , Feidt Michel , Dobre Catalina , Borcila Bogdan
Published: April 2021

An irreversible Carnot cycle engine operating as a closed system is modeled using the Direct Method and the First Law of Thermodynamics for processes with Finite Speed. Several models considering the effect on the engine performance of external and internal irreversibilities expressed as a function of the piston speed are presented. External irreversibilities are due to heat transfer at temperature gradient between the cycle and heat reservoirs, while internal ones are represented by pressure losses due to the finite speed of the piston and friction. Moreover, a method for optimizing the temperature of the cycle fluid with respect to the temperature of source and sink and the piston speed is provided. The optimization results predict distinct maximums for the thermal efficiency and power output, as well as different behavior of the entropy generation per cycle and per time. The results obtained in this optimization, which is based on piston speed, and the Curzon–Ahlborn optimization, which is based on time duration, are compared and are found to differ significantly. Correction have been proposed in order to include internal irreversibility in the externally irreversible Carnot cycle from Curzon–Ahlborn optimization, which would be equivalent to a unification attempt of the two optimization analyses.
Some Tips on Numerical Modeling of Airflow and Fires in Road Tunnels
Authors: Król Aleksander , Król Małgorzata
Published: April 2021

The efficiency of tunnels systems is often evaluated using numerical simulations. This concerns both to normal and emergency mode of tunnel systems operation. Therefore the safety level of tunnel users may depend on the quality of numerical models being built. The most often studied areas cover the researches on natural and forced airflows in the normal mode and on fire development and smoke spreading in the emergency mode as well as modeling of fan operation. Thus, many software packages implementing Computational Fluid Dynamics (CFD) are applied here. Despite the available software is recognized as reliable, the problem arises because the built numerical models should be validated at least partially with experimental data. There is a shortage of experimental data from real tunnels due to high costs and many organizational or formal difficulties. Some researchers use data from scaled experiments, but this leads to problems connected with scaling. The paper presents the application of two widely used software packages—Fire Dynamics Simulator (FDS) and ANSYS Fluent to reproduce some scenarios of the operation of a tunnel ventilation system for normal and emergency mode. Most of results were compared with data obtained by own full scale measurements or data available in literature. Some practical issues concerning the application of FDS and ANSYS Fluent were discussed as well.
Estimation of Tire Normal Forces including Suspension Dynamics
Authors: Filipozzi Louis , Assadian Francis , Kuang Ming , Johri Rajit , Velazquez Alcantar Jose
Published: April 2021

Tire normal forces are difficult to measure, but information on the vehicle normal force can be used in many automotive engineering applications, e.g., rollover detection and vehicle and wheel stability. Previous papers use algebraic equations to estimate the tire normal force. In this article, the estimation of tire normal force is formulated as an input estimation problem. Two observers are proposed to solve this problem by using a quarter-car suspension model. First, the Youla Controller Output Observer framework is presented. It converts the estimation problem into a control problem and produces a Youla parameterized controller as observer. Second, a Kalman filter approach is taken and the input estimation problem is addressed with an Unbiased Minimum Variance Filter. Both methods use accelerometer and suspension deflection sensors to determine the vehicle normal force. The design of the observers is validated in simulation and a sensitivity analysis is performed to evaluate their robustness.
Energy-Aware Scheduling Based on Marginal Cost and Task Classification in Heterogeneous Data Centers
Authors: Ji Kaixuan , Chi Ce , Zhang Fa , Anta Antonio Fernández , Song Penglei , Marahatta Avinab , Wang Youshi , Liu Zhiyong
Published: April 2021

The energy consumption problem has become a bottleneck hindering further development of data centers. However, the heterogeneity of servers, hybrid cooling modes, and extra energy caused by system state transitions increases the complexity of the energy optimization problem. To deal with such challenges, in this paper, an Energy Aware Task Scheduling strategy (EATS) utilizing marginal cost and task classification method is proposed that cooperatively improves the energy efficiency of servers and cooling systems. An energy consumption model for servers, cooling systems, and state transition is developed, and the energy optimization problem in data centers is formulated. The concept of marginal cost is introduced to guide the task scheduling process. The task classification method is incorporated with the idea of marginal cost to further improve resource utilization and reduce the total energy consumption of data centers. Experiments are conducted using real-world traces, and energy reduction results are compared. Results show that EATS achieves more energy-savings of servers, cooling systems, state transition in comparison to the other two techniques under a various number of servers, cooling modules and task arrival intensities. It is validated that EATS is effective at reducing total energy consumption and improving the resource utilization of data centers.
Spatial Modeling of Potential Lobster Harvest Grounds in Palabuhanratu Bay, West Java, Indonesia
Authors: Mukhtar Mutia Kamalia , Manessa Masita Dwi Mandini , Supriatna Supriatna , Khikmawati Liya Tri
Published: April 2021

Palabuhanratu Bay is a location in the southern part of Java Island with a high lobster population. Based on field observation, the lobster population in Palabuhanratu Bay is dominated by Panulirus homarus (green sand lobster), Panulirus versicolor (bamboo lobster), Panulirus penicillatus (black lobster), and Panulirus ornatus (pearl lobster). This study aimed to develop a spatial model using satellite-derived data to predict potential lobster harvest grounds in Palabuhanratu Bay. The Earth observational satellite data used were multispectral Landsat 8-SR imagery, and information about chlorophyll-a, salinity, total suspended solids (TSSs), sea surface temperature (SST), and distance from the coastline was extracted. Multiple linear regression was applied to build the prediction model, which was validated using 10-fold cross-validation. The result of the lobster harvest prediction model agreed with the root-mean-square error (RMSE) and adjusted R2 values of 0.326 and 0.708, respectively. The distribution of lobsters was strong at the following preferred ranges: chlorophyll-a: 1.1–1.7 mg/m3; salinity: 20.2–23.7 ppt; TSS: 40–56.4 mg/L; SST: 29.5–29.9 °C; and distance from the coastline: 500–4700 m. In this study, the habitats of four species of lobsters and their relationships with satellite-derived parameters were evaluated.
Characterization of the Triacylglycerol Fraction of Italian and Extra-European Hemp Seed Oil
Authors: Tringaniello Carmela , Cossignani Lina , Blasi Francesca
Published: April 2021

Hemp seed oil (HSO) has received considerable attention for its health properties, especially due to unsaturated fatty acid (UFA) content. In this work, the triacylglycerol (TAG) fraction of Italian and Extra-European HSO was characterized by applying an enzymatic approach, based on the use of pancreatic lipase and sn-1,2-diacylglycerol kinase. This procedure allows determination of the intrapositional FA% composition of TAG. The results of the stereospecific analysis are useful for deepening knowledge on HSO nutritional aspects. The high percentage of UFA (88.3–89.9%), in particular essential FA (74.4–85.9%), of HSO samples in sn-2 position is important for long-term health effects, but also to enhance the use of this oil as a functional ingredient in food, cosmetic and nutraceutical fields. Furthermore, the results of total and intrapositional FA % compositions, subjected to principal component analysis, were able to differentiate HSO Italian samples from Extra-European ones. Based on the obtained results, it can be stated that the stereospecific analysis represents a potent analytical tool providing the fingerprint of TAG fraction, useful to highlight possible chemical descriptors for HSO authenticity and traceability purposes.
Gluten-Free Breadsticks Fortified with Phenolic-Rich Extracts from Olive Leaves and Olive Mill Wastewater
Authors: Conte Paola , Pulina Simone , Del Caro Alessandra , Fadda Costantino , Urgeghe Pietro Paolo , De Bruno Alessandra , Difonzo Graziana , Caponio Francesco , Romeo Rosa , Piga Antonio
Published: April 2021

Nowadays, food processing by-products, which have long raised serious environmental concerns, are recognized to be a cheap source of valuable compounds. In the present study, incorporation of phenolic-rich extracts (500 and 1000 mg kg−1) from olive leaves (OL) and olive mill wastewater (OMW) into conventional gluten-free formulations has been exploited as a potential strategy for developing nutritious and healthy breadsticks with extended shelf-life. To this end, moisture, water activity (aw), visual and textural properties, the composition of biologically active compounds (soluble, insoluble, and bio-accessible polyphenols), antioxidant activity, oxidation stability, and consumer preference of the resulting breadsticks were investigated. Fortified breadsticks had higher moisture and aw, lower hardness, and similar color in comparison to the control, especially in the case of OL extract supplementation. All enriched formulations significantly affected the phenolic composition, as evidenced by the decrease in insoluble/soluble polyphenols ratio (from 7 in the control up to 3.1 and 4.5 in OL and OMW, respectively), and a concomitant increase in polyphenol bio-accessibility (OL: 14.5–23% and OMW: 10.4–15% rise) and antioxidant activity (OL: 20–36% and OMW: 11–16% rise). Moreover, a significant shelf-life extension was observed in all fortified breadsticks (especially in case of OMW supplementation). Sensory evaluation evidenced that 61% of the assessors showed a marked, but not significant, tendency to consider the sample supplemented with high levels of OL as a more palatable choice.
Theory of Charged Gels: Swelling, Elasticity, and Dynamics
Authors: Jia Di , Muthukumar Murugappan
Published: April 2021

The fundamental attributes of charged hydrogels containing predominantly water and controllable amounts of low molar mass electrolytes are of tremendous significance in biological context and applications in healthcare. However, a rigorous theoretical formulation of gel behavior continues to be a challenge due to the presence of multiple length and time scales in the system which operate simultaneously. Furthermore, chain connectivity, the electrostatic interaction, and the hydrodynamic interaction all lead to long-range interactions. In spite of these complications, considerable progress has been achieved over the past several decades in generating theories of variable complexity. The present review presents an analytically tractable theory by accounting for correlations emerging from topological, electrostatic, and hydrodynamic interactions. Closed-form formulas are derived for charged hydrogels to describe their swelling equilibrium, elastic moduli, and the relationship between microscopic properties such as gel diffusion and macroscopic properties such as elasticity. In addition, electrostatic coupling between charged moieties and their ion clouds, which significantly modifies the elastic diffusion coefficient of gels, and various scaling laws are presented. The theoretical formulas summarized here are useful to adequately capture the essentials of the physics of charged gels and to design new hydrogels with specified elastic and dynamical properties.
Common Factors in the Term Structure of Credit Spreads and Predicting the Macroeconomy in Japan
Authors: Kobayashi Takeshi
Published: April 2021

This study extracts the common factors from firm-based credit spreads of major Japanese corporate bonds and examines the predictive content of the credit spread on the real economy. Instead of employing single-maturity corporate bond spreads, we focus on the entire term structure of the credit spread to predict the business cycle. We extend the dynamic Nelson-Siegel model to allow for both common and firm-specific factors. The results show that the estimated common factors are important drivers of individual credit spreads and have substantial predictive power for future Japanese economic activity. This study contributes to the literature by examining the relationship between firm-based credit spread curves and economic fluctuation and forecasting the business cycle.
Effects of Whole-Body Adenylyl Cyclase 5 (Adcy5) Deficiency on Systemic Insulin Sensitivity and Adipose Tissue
Authors: Dommel Sebastian , Hoffmann Anne , Berger Claudia , Kern Matthias , Klöting Nora , Kannt Aimo , Blüher Matthias
Published: April 2021

Genome-wide association studies have identified adenylyl cyclase type 5 (ADCY5) as candidate gene for diabetes-related quantitative traits and an increased risk of type 2 diabetes. Mice with a whole-body deletion of Adcy5 (Adcy5–/–) do not develop obesity, glucose intolerance and insulin resistance, have improved cardiac function and increased longevity. Here, we investigated Adcy5 knockout mice (Adcy5–/–) to test the hypothesis that changes in adipose tissue (AT) may contribute to the reported healthier phenotype. In contrast to previous reports, we found that deletion of Adcy5 did not confer any physiological or biochemical benefits. However, this unexpected finding allowed us to investigate the effects of Adcy5 depletion on AT independently of lower body weight and a metabolically healthier phenotype. Adcy5–/– mice exhibited an increased number of smaller adipocytes, lower mean adipocyte size and a distinct AT gene expression pattern with midline 1 (Mid1) as the most significantly downregulated gene compared to control mice. Our Adcy5–/– model challenges previously described beneficial effects of Adcy5 deficiency and suggests that targeting Adcy5 does not improve insulin sensitivity and may therefore limit the relevance of ADCY5 as potential drug target.
Inhalational Anesthetics Inhibit Neuroglioma Cell Proliferation and Migration via miR-138, -210 and -335
Authors: Ishikawa Masashi , Iwasaki Masae , Zhao Hailin , Saito Junichi , Hu Cong , Sun Qizhe , Sakamoto Atsuhiro , Ma Daqing
Published: April 2021

Inhalational anesthetics was previously reported to suppress glioma cell malignancy but underlying mechanisms remain unclear. The present study aims to investigate the effects of sevoflurane and desflurane on glioma cell malignancy changes via microRNA (miRNA) modulation. The cultured H4 cells were exposed to 3.6% sevoflurane or 10.3% desflurane for 2 h. The miR-138, -210 and -335 expression were determined with qRT-PCR. Cell proliferation and migration were assessed with wound healing assay, Ki67 staining and cell count kit 8 (CCK8) assay with/without miR-138/-210/-335 inhibitor transfections. The miRNA downstream proteins, hypoxia inducible factor-1α (HIF-1α) and matrix metalloproteinase 9 (MMP9), were also determined with immunofluorescent staining. Sevoflurane and desflurane exposure to glioma cells inhibited their proliferation and migration. Sevoflurane exposure increased miR-210 expression whereas desflurane exposure upregulated both miR-138 and miR-335 expressions. The administration of inhibitor of miR-138, -210 or -335 inhibited the suppressing effects of sevoflurane or desflurane on cell proliferation and migration, in line with the HIF-1α and MMP9 expression changes. These data indicated that inhalational anesthetics, sevoflurane and desflurane, inhibited glioma cell malignancy via miRNAs upregulation and their downstream effectors, HIF-1α and MMP9, downregulation. The implication of the current study warrants further study.
Biomarkers in Prostate Cancer Diagnosis: From Current Knowledge to the Role of Metabolomics and Exosomes
Authors: Salciccia Stefano , Capriotti Anna Laura , Laganà Aldo , Fais Stefano , Logozzi Mariantonia , De Berardinis Ettore , Busetto Gian Maria , Di Pierro Giovanni Battista , Ricciuti Gian Piero , Del Giudice Francesco , Sciarra Alessandro , Carroll Peter R. , Cooperberg Matthew R. , Sciarra Beatrice , Maggi Martina
Published: April 2021

Early detection of prostate cancer (PC) is largely carried out using assessment of prostate-specific antigen (PSA) level; yet it cannot reliably discriminate between benign pathologies and clinically significant forms of PC. To overcome the current limitations of PSA, new urinary and serum biomarkers have been developed in recent years. Although several biomarkers have been explored in various scenarios and patient settings, to date, specific guidelines with a high level of evidence on the use of these markers are lacking. Recent advances in metabolomic, genomics, and proteomics have made new potential biomarkers available. A number of studies focused on the characterization of the specific PC metabolic phenotype using different experimental approaches has been recently reported; yet, to date, research on metabolomic application for PC has focused on a small group of metabolites that have been known to be related to the prostate gland. Exosomes are extracellular vesicles that are secreted from all mammalian cells and virtually detected in all bio-fluids, thus allowing their use as tumor biomarkers. Thanks to a general improvement of the technical equipment to analyze exosomes, we are able to obtain reliable quantitative and qualitative information useful for clinical application. Although some pilot clinical investigations have proposed potential PC biomarkers, data are still preliminary and non-conclusive.
The Role of NRF2/KEAP1 Signaling Pathway in Cancer Metabolism
Authors: Song Moon-Young , Lee Da-Young , Chun Kyung-Soo , Kim Eun-Hee
Published: April 2021

The nuclear factor-erythroid 2 p45-related factor 2 (NRF2, also called Nfe2l2) and its cytoplasmic repressor, Kelch-like ECH-associated protein 1 (KEAP1), are major regulators of redox homeostasis controlling a multiple of genes for detoxification and cytoprotective enzymes. The NRF2/KEAP1 pathway is a fundamental signaling cascade responsible for the resistance of metabolic, oxidative stress, inflammation, and anticancer effects. Interestingly, a recent accumulation of evidence has indicated that NRF2 exhibits an aberrant activation in cancer. Evidence has shown that the NRF2/KEAP1 signaling pathway is associated with the proliferation of cancer cells and tumerigenesis through metabolic reprogramming. In this review, we provide an overview of the regulatory molecular mechanism of the NRF2/KEAP1 pathway against metabolic reprogramming in cancer, suggesting that the regulation of NRF2/KEAP1 axis might approach as a novel therapeutic strategy for cancers.
Role of Short Chain Fatty Acids and Apolipoproteins in the Regulation of Eosinophilia-Associated Diseases
Authors: Sturm Eva Maria , Knuplez Eva , Marsche Gunther
Published: April 2021

Eosinophils are key components of our host defense and potent effectors in allergic and inflammatory diseases. Once recruited to the inflammatory site, eosinophils release their cytotoxic granule proteins as well as cytokines and lipid mediators, contributing to parasite clearance but also to exacerbation of inflammation and tissue damage. However, eosinophils have recently been shown to play an important homeostatic role in different tissues under steady state. Despite the tremendous progress in the treatment of eosinophilic disorders with the implementation of biologics, there is an unmet need for novel therapies that specifically target the cytotoxic effector functions of eosinophils without completely depleting this multifunctional immune cell type. Recent studies have uncovered several endogenous molecules that decrease eosinophil migration and activation. These include short chain fatty acids (SCFAs) such as butyrate, which are produced in large quantities in the gastrointestinal tract by commensal bacteria and enter the systemic circulation. In addition, high-density lipoprotein-associated anti-inflammatory apolipoproteins have recently been shown to attenuate eosinophil migration and activation. Here, we focus on the anti-pathogenic properties of SCFAs and apolipoproteins on eosinophil effector function and provide insights into the potential use of SCFAs and apolipoproteins (and their mimetics) as effective agents to combat eosinophilic inflammation.
A Review on Combination of Ab Initio Molecular Dynamics and NMR Parameters Calculations
Authors: Mazurek Anna Helena , Szeleszczuk Łukasz , Pisklak Dariusz Maciej
Published: April 2021

This review focuses on a combination of ab initio molecular dynamics (aiMD) and NMR parameters calculations using quantum mechanical methods. The advantages of such an approach in comparison to the commonly applied computations for the structures optimized at 0 K are presented. This article was designed as a convenient overview of the applied parameters such as the aiMD type, DFT functional, time step, or total simulation time, as well as examples of previously studied systems. From the analysis of the published works describing the applications of such combinations, it was concluded that including fast, small-amplitude motions through aiMD has a noticeable effect on the accuracy of NMR parameters calculations.
The Emerging Clinical Role of Spermine in Prostate Cancer
Authors: Peng Qiang , Wong Christine Yim-Ping , Cheuk Isabella Wai-yin , Teoh Jeremy Yuen-Chun , Chiu Peter Ka-Fung , Ng Chi-Fai
Published: April 2021

Spermine, a member of polyamines, exists in all organisms and is essential for normal cell growth and function. It is highly expressed in the prostate compared with other organs and is detectable in urine, tissue, expressed prostatic secretions, and erythrocyte. A significant reduction of spermine level was observed in prostate cancer (PCa) tissue compared with benign prostate tissue, and the level of urinary spermine was also significantly lower in men with PCa. Decreased spermine level may be used as an indicator of malignant phenotype transformation from normal to malignant tissue in prostate. Studies targeting polyamines and key rate-limiting enzymes associated with spermine metabolism as a tool for PCa therapy and chemoprevention have been conducted with various polyamine biosynthesis inhibitors and polyamine analogues. The mechanism between spermine and PCa development are possibly related to the regulation of polyamine metabolism, cancer-driving pathways, oxidative stress, anticancer immunosurveillance, and apoptosis regulation. Although the specific mechanism of spermine in PCa development is still unclear, ongoing research in spermine metabolism and its association with PCa pathophysiology opens up new opportunities in the diagnostic and therapeutic roles of spermine in PCa management.
Inflammasomes in Teleosts: Structures and Mechanisms That Induce Pyroptosis during Bacterial Infection
Authors: Morimoto Natsuki , Kono Tomoya , Sakai Masahiro , Hikima Jun-ichi
Published: April 2021

Pattern recognition receptors (PRRs) play a crucial role in inducing inflammatory responses; they recognize pathogen-associated molecular patterns, damage-associated molecular patterns, and environmental factors. Nucleotide-binding oligomerization domain-leucine-rich repeat-containing receptors (NLRs) are part of the PRR family; they form a large multiple-protein complex called the inflammasome in the cytosol. In mammals, the inflammasome consists of an NLR, used as a sensor molecule, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) as an adaptor protein, and pro-caspase1 (Casp1). Inflammasome activation induces Casp1 activation, promoting the maturation of proinflammatory cytokines, such as interleukin (IL)-1β and IL-18, and the induction of inflammatory cell death called pyroptosis via gasdermin D cleavage in mammals. Inflammasome activation and pyroptosis in mammals play important roles in protecting the host from pathogen infection. Recently, numerous inflammasome-related genes in teleosts have been identified, and their conservation and/or differentiation between their expression in mammals and teleosts have also been elucidated. In this review, we summarize the current knowledge of the molecular structure and machinery of the inflammasomes and the ASC-spec to induce pyroptosis; moreover, we explore the protective role of the inflammasome against pathogenic infection in teleosts.