bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Impacts of Clove Essential Oil Nanoemulsion as Bio Fungicides against Neoscytalidium Blight Disease of Carum carvi L.
Authors: Hashem Amr H. , Abdelaziz Amer M. , Hassanin Mahmoud M. H. , Al-Askar Abdulaziz A. , AbdElgawad Hamada , Attia Mohamed S.
Published: April 2023

Fungal plant diseases are considered one of the most destructive diseases for plants. In this current study, Neoscytalidium dimidiatum as a fungal plant pathogen was isolated from an infected Carum carvi L. plant (C. carvi) for the first time and controlled it using clove essential oil nanoemulsion (CEONE). This fungal plant pathogen was identified morphologically and genetically, then deposited in the gene bank under accession number OQ338188. CEONE was prepared and characterized using DLS, Zeta potential, and TEM analysis. The characterization results illustrated that the size of CEONE was lower than 100 nm, which was confirmed by TEM, which appeared spherical in shape. Results revealed that CEONE has promising antifungal activity towards N. dimidiatum, where inhibition percentages of CEONE at concentrations of 5000, 3000, and 1000 ppm were 82.2%, 53.3%, and 25.5%, respectively. At the pot level, N. dimidiatum was highly virulent on C. carvi, and CEONE and CEO were highly effective against N. dimidiatum blight disease, with 71.42% and 57.14% protection, respectively. Moreover, results revealed that there was a vast improvement in root length, plant height, and leaves. Additionally, the application of CEONE and CEO induced plant resistance by modifying proline, phenol, hydrogen peroxide, malondialdehyde, and antioxidant enzymes. In conclusion, N. dimidiatum was highly virulent on C. carvi but can be effectively reduced by CEONE through the eco-friendly method.
Use of a Pair of Pulse-Charged Grounded Metal Nets as an Electrostatic Soil Cover for Eradicating Weed Seedlings
Authors: Matsuda Yoshinori , Takikawa Yoshihiro , Shimizu Kunihiko , Kusakari Shin-ichi , Toyoda Hideyoshi
Published: April 2023

An electrostatic technique was developed to generate a simple physical method to eradicate weeds in crop fields. The proposed apparatus consisted of double-expanded metal nets connected to a pulse-charging type negative voltage generator and a grounded line. The two metal nets were arranged in parallel at an interval (6 mm) that caused no arc (spark) discharge between the negatively charged metal net (NC-MN) and the grounded metal net (G-MN). The paired nets were used as a soil cover to zap weed seedlings emerging from the ground. As plant seedlings are biological conductors, the seedling was subjected to an arc discharge from the upper metal net (NC-MN) when it emerged from the soil and passed through the lower net (G-MN). The discharge was strong enough to destroy the seedling with a single exposure. The arc treatment was highly effective for eradicating successively emerging mono- and dicotyledonous weed seedlings, regardless of the number of coexisting weeds or the area of the netted field. Thus, the present study provides a simple and reliable weed eradication method that could be integrated into a sustainable crop production system.
Initial Weed and Maize Response to Conservation Tillage and Liming in Different Agroecological Conditions
Authors: Brozović Bojana , Jug Irena , Đurđević Boris , Ravlić Marija , Vukadinović Vesna , Rojnica Iva , Jug Danijel
Published: April 2023

Conservation tillage (CT) is an effective tool for maintaining crop productivity under adverse climate conditions, while its adoption is conditioned by the possible negative response of crop weed. Research with CT and liming (L) was conducted at different experimental sites on acid soils (ES 1 and ES 2) to determine the maize weediness and yield. The tillage treatments used were ST (conventional tillage), CTD (deep loosening), CTS (shallow loosening), and liming; Ly (CaO) and Ln (no CaO). The weediness assessment was conducted at the V7 and R5 maize growth stages. Weed density (WD), biomass (WB), weed coverage (WC), and species density (WSN) were determined. The highest WD was recorded on ES 2 in V7, and WB, WC, and WSN were significantly higher at CTS in R5 compared to ST. Liming affected the decrease of WD and WC in V7 and WB, WC, and WSN in R5. The average maize yield on ES 2 was 36% higher compared to ES 1. CTS resulted with the highest yield at ES 1, while at ES 2, it was similar to ST. Liming application significantly increased the maize yield. The given results indicated the positive impact of CT and L on crop productivity in different agroecological conditions, despite the increased weediness.
Effects of Water and Nitrogen Management on Root Morphology, Nitrogen Metabolism Enzymes, and Yield of Rice under Drip Irrigation
Authors: Tang Qingyun , Ma Yadong , Zhao Lei , Song Zhiwen , Yin Yongan , Wang Guodong , Li Yuxiang
Published: April 2023

This paper explores the effects of water and nitrogen management on drip irrigated rice root morphology, nitrogen metabolism and yield, clarifies the relationship between root characteristics and yield formation. Normal irrigation (W1, 10,200 m3/hm2) and limited irrigation (W2, 8670 m3/hm2, 85% of W1) were set with nitrogen-efficient variety (T-43) and nitrogen-inefficient variety (LX-3) as the materials. Under the condition of a total nitrogen application rate of 300 kg/hm2, three kinds of nitrogen management methods were applied, N1: a seedling: tiller: panicle: grain ratio of 30%:50%:13%:7%; N2: a ratio of 20%:40%:30%:10%; and N3: 10%:30%:40%:20%. Their effects on root morphology, root architecture, and nitrogen metabolism enzyme activities were studied. The results showed, drip irrigated rice yields were highest under W1N2, reaching 9.0 t/hm2 for T-43 and 7.3 t/hm2 for LX-3. Compared with W2, the root length density (RLD), surface area density (SAD), and root volume density (RVD) of finely branched roots, coarsely branched roots and adventitious roots increased by 49.5%, 44.6%, and 46.7%; the RLD, SAD, RVD, and root architecture RLD β values of the 0–30-cm soil layer increased significantly (p < 0.05); and the yield and nitrogen partial factor productivity increased by 20.7% and 23.3%, respectively, under W1. Compared with N1, RLD, SAD and RVD in 0–10 cm soil layer under N2 increased significantly by 24.8%, 35.6% and 31.4%, and RLDβ decreased significantly (p < 0.05); Leaf GS, GOGAT and GDH were increased by 37.9%, 17.0% and 40.9%; all indexes showed a downward trend under N3. Compared with LX-3, T-43 RLD, SAD, RVD increased significantly (p < 0.05), nitrogen metabolism enzyme activity increased, and yield increased by 21.8%. Rational water and nitrogen management can optimize the root growth and distribution characteristics and achieve simultaneous improvement of rice yield, nitrogen absorption, and nitrogen utilization efficiency under drip irrigation.
Deep Learning YOLO-Based Solution for Grape Bunch Detection and Assessment of Biophysical Lesions
Authors: Pinheiro Isabel , Moreira Germano , Queirós da Silva Daniel , Magalhães Sandro , Valente António , Moura Oliveira Paulo , Cunha Mário , Santos Filipe
Published: April 2023

The world wine sector is a multi-billion dollar industry with a wide range of economic activities. Therefore, it becomes crucial to monitor the grapevine because it allows a more accurate estimation of the yield and ensures a high-quality end product. The most common way of monitoring the grapevine is through the leaves (preventive way) since the leaves first manifest biophysical lesions. However, this does not exclude the possibility of biophysical lesions manifesting in the grape berries. Thus, this work presents three pre-trained YOLO models (YOLOv5x6, YOLOv7-E6E, and YOLOR-CSP-X) to detect and classify grape bunches as healthy or damaged by the number of berries with biophysical lesions. Two datasets were created and made publicly available with original images and manual annotations to identify the complexity between detection (bunches) and classification (healthy or damaged) tasks. The datasets use the same 10,010 images with different classes. The Grapevine Bunch Detection Dataset uses the Bunch class, and The Grapevine Bunch Condition Detection Dataset uses the OptimalBunch and DamagedBunch classes. Regarding the three models trained for grape bunches detection, they obtained promising results, highlighting YOLOv7 with 77% of mAP and 94% of the F1-score. In the case of the task of detection and identification of the state of grape bunches, the three models obtained similar results, with YOLOv5 achieving the best ones with an mAP of 72% and an F1-score of 92%.
Residual Carbon Derived from Different Maize Parts Differed in Soil Organic Carbon Fractions as Affected by Soil Fertility
Authors: Wang Mengmeng , Pei Jiubo , Yu Yaxi , Wang Siyin
Published: April 2023

Maize straw returning is one of the important measures to improve dryland soil organic carbon (SOC). However, the effects of different maize parts on SOC fractions with different soil fertility levels in situ are not exactly clear. Therefore, an in situ field incubation experiment over 540 days, by adding different 13C-labeled maize parts (root, stem and leaf) into low- (no fertilizer treatment) and high- (manure treatment) fertility soils, was conducted at a long-term brown earth experimental site in Shenyang of China to figure out the effects of different maize parts on SOC fractions (dissolved organic carbon (DOC) and particulate organic carbon (POC)). The results showed that the distribution–DOC ratio of low-fertility treatment was higher than that of high-fertility treatment in the period of rapid decomposition of straw. In both low- and high-fertility soils, the ratio of carbon to DOC in leaf residue was higher than that in root and stem residues. The proportion of root, stem and leaf residue converted to DOC in low-fertility soil was 4.51%, 3.89% and 5.00%, respectively. The proportion of root, stem and leaf residue converted to DOC in high-fertility soil was 4.10%, 3.65% and 4.11%, respectively. As for the distribution–POC ratio, during the period of rapid decomposition of straw, the ratio of carbon conversion from root and stem residue to POC was generally higher than that from leaf residue. The ratio of carbon conversion to POC of root, stem and leaf residues in high-fertility treatment was higher than that in low-fertility treatment. In low-fertility treatment, the proportion of root, stem and leaf residues converted to POC was 41.34%, 46.33% and 36.11%, respectively. The proportion of root, stem and leaf residue converted to POC in high-fertility soil was 46.48%, 44.45% and 41.14%, respectively. The results showed that, for DOC, a low fertility level and more leaf residue types were beneficial. While, for POC, root and stem residues with a high fertility level were beneficial. These results provide evidence that the addition of different parts of maize residues would have differing effects on DOC and POC. Leaf residues in low-fertility soils were more suitable for increasing DOC. Root and stem residues in high-fertility soils were more suitable for increasing POC. Nevertheless, we could not ignore the unmeasured SOC fractions that some of the residues could be converted to.
Droughts and Thermo-Priming Enhance Acclimation to Later Drought and Heat Stress in Maize Seedlings by Improving Leaf Physiological Activity
Authors: Ru Chen , Hu Xiaotao , Chen Dianyu , Wang Wene
Published: April 2023

Early heat and drought priming may increase the plant’s ability to resist later drought and heat stress. However, it remains unclear whether combined heat and drought priming can enhance the acclimation of plants to later combined stress by improving physiological activities. In this study, maize seedlings were first pre-exposed twice to heat, drought, and a combination of stresses followed by recovery, and then subjected to six days of more severe stresses. A considerable reduction in photosynthetic pigment content, stomatal size, and photosynthesis was observed under heat and drought conditions, and the changes in the above indicators were amplified under combined stress conditions. Stress priming improves antioxidant defense and cellular osmoregulation, as indicated by improved superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione reductase (GR), and ascorbate peroxidase activities, as well as elevated soluble sugar (SS) and proline (Pro) contents. Lower superoxide anion and malondialdehyde contents and injury index in the primed seedlings demonstrated the mitigation of oxidative stress. ROC analysis revealed that SOD and POD had considerable reliability in determining that maize seedlings were experiencing heat stress (AUC = 0.941–0.971); GR and SS were capable of accurately monitoring drought stress that was being experienced by plants (AUC = 0.919–0.958); and SOD, GR, and Pro had more capability for detecting the combination of heat and drought stress (AUC = 0.907–0.958). Collectively, the primed seedlings exhibited better performance than the non-primed seedlings, exhibiting stronger stress acclimation supported by an effective antioxidant defense system and osmoregulatory function.
SheepInst: A High-Performance Instance Segmentation of Sheep Images Based on Deep Learning
Authors: Zhao Hongke , Mao Rui , Li Mei , Li Bin , Wang Meili
Published: April 2023

Sheep detection and segmentation will play a crucial role in promoting the implementation of precision livestock farming in the future. In sheep farms, the characteristics of sheep that have the tendency to congregate and irregular contours cause difficulties for computer vision tasks, such as individual identification, behavior recognition, and weight estimation of sheep. Sheep instance segmentation is one of the methods that can mitigate the difficulties associated with locating and extracting different individuals from the same category. To improve the accuracy of extracting individual sheep locations and contours in the case of multiple sheep overlap, this paper proposed two-stage sheep instance segmentation SheepInst based on the Mask R-CNN framework, more specifically, RefineMask. Firstly, an improved backbone network ConvNeXt-E was proposed to extract sheep features. Secondly, we improved the structure of the two-stage object detector Dynamic R-CNN to precisely locate highly overlapping sheep. Finally, we enhanced the segmentation network of RefineMask by adding spatial attention modules to accurately segment irregular contours of sheep. SheepInst achieves 89.1%, 91.3%, and 79.5% in box AP, mask AP, and boundary AP metric on the test set, respectively. The extensive experiments show that SheepInst is more suitable for sheep instance segmentation and has excellent performance.
New Insight into the Quality Traits of Milk and Cheese from Teramana Goats, a Native Italian Breed
Authors: Florio Marco , Cimini Costanza , Ianni Andrea , Bennato Francesca , Grotta Lisa , Valbonetti Luca , Martino Giuseppe
Published: April 2023

The preservation and enhancement of native breeds is a central issue to initiate new breeding policies, which are sustainable and adapted to climate changes. The aim of this study was the characterisation of the qualitative traits of milk and cheese obtained from Teramana goats compared with Saanen goats reared in the same breeding facilities or environment. The research involved 41 Teramana goats and 40 Saanen goats. The milk of each group was collected and used to produce cheese, which was analysed fresh and after 30 and 60 days of ripening. Cheese samples were subjected to evaluations of the physical parameters, including colour and the TPA test, in addition to chemical evaluations that were focused on the determination of total lipids, fatty acids composition, volatile profile and proteolysis. The results showed the Teramana goat to be rich in fat, characterised by a significant increase in conjugates of linoleic acid (CLA), which are attributed to important health benefits. The analysis of volatile compounds showed more oxidative stability of Teramana goats’ cheeses during the ripening. The results from sensory analyses indicated an improved hardness and yellowness, which could be accompanied by an improvement in customer acceptance. In conclusion, our study shows interesting results regarding the milk and cheese from the Teramana goat, as well as a positive evaluation by consumers, findings that encourage the importance of promoting native breeds.
Survival, Growth Performance, and Hepatic Antioxidant and Lipid Profiles in Infected Rainbow Trout (Oncorhynchus mykiss) Fed a Diet Supplemented with Dihydroquercetin and Arabinogalactan
Authors: Sukhovskaya Irina V. , Lysenko Liudmila A. , Fokina Natalia N. , Kantserova Nadezhda P. , Borvinskaya Ekaterina V.
Published: April 2023

Natural feed supplements have been shown to improve fish viability, health, and growth, and the ability to withstand multiple stressors related to intensive cultivation. We assumed that a dietary mix of plant-origin substances, such as dihydroquercetin, a flavonoid with antioxidative, anti-inflammatory, and antimicrobial properties, and arabinogalactan, a polysaccharide with immunomodulating activity, would promote fish stress resistance and expected it to have a protective effect against infectious diseases. Farmed rainbow trout fish, Oncorhynchus mykiss, received either a standard diet or a diet supplemented with 25 mg/kg of dihydroquercetin and 50 mg/kg of arabinogalactan during a feeding season, from June to November. The fish in the control and experimental groups were sampled twice a month (eight samplings in total) for growth variable estimations and tissue sampling. The hepatic antioxidant status was assessed via the quantification of molecular antioxidants, such as reduced glutathione and alpha-tocopherol rates, as well as the enzyme activity rates of peroxidase, catalase, and glutathione-S-transferase. The lipid and fatty acid compositions of the feed and fish liver were analyzed using thin-layer and high-performance liquid chromatography. The viability, size, and biochemical indices of the fish responded to the growth physiology, environmental variables such as the dissolved oxygen content and water temperature, and sporadic factors. Due to an outbreak of a natural bacterial infection in the fish stock followed by antibiotic treatment, a higher mortality rate was observed in the fish that received a standard diet compared to those fed supplemented feed. In the postinfection period, reduced dietary 18:2n-6 and 18:3n-3 fatty acid assimilation contents were detected in the fish that received the standard diet in contrast to the supplemented diet. By the end of the feeding season, an impaired antioxidant response, including reduced glutathione S-transferase activity and glutathione content, and a shift in the composition of membrane lipids, such as sterols, 18:1n-7 fatty acid, and phospholipids, were also revealed in fish fed the standard diet. Dietary supplementation with plant-origin substances, such as dihydroquercetin and arabinogalactan, decreases lethality in fish stocks, presumably though the stimulation of natural resistance in farmed fish, thereby increasing the economic efficacy during fish production. From the sustainable aquaculture perspective, natural additives also diminish the anthropogenic transformation of aquaculture-bearing water bodies and their ecosystems.
Dietary Supplementation with Linseed Oil Ethyl Esters Improves Sexual Behavior and Chosen Seminal Parameters in Porcine Species
Authors: Zwyrzykowska-Wodzińska Anna , Bielas Wiesław , Niżański Wojciech , Jankowska-Mąkosa Anna , Knecht Damian
Published: April 2023

Numerous studies have shown that improvements in the sperm and semen quality of males of many species can be achieved with appropriate dietary supplements added to feed or fodder. Particularly promising seems to be the inclusion of omega polyunsaturated fatty acids in the diets of males. Among other things, it has been shown that linseed oil ethyl esters (EELO can be an excellent source of omega 3 polyunsaturated fatty acids in animal diets. These compounds are more durable and resistant to oxidation, epoxidation and resinification processes, and do not exhibit toxic properties in living organisms. At present, there is a lack of data in the literature on the enrichment of boar diets with EELO. The purpose of this study was to analyze the effects of the addition of EELO to boar diets on the properties of sperm in fresh semen. The study was conducted during the summer on semen collected from 12 boars of the line 990. Linseed oil ethyl esters were administered in each feeding at a rate of 3.0% (45 mL each) in basal diets for each boar on a daily basis for 16 weeks. Ejaculates were collected manually by the gloved-hand technique, at one-week intervals for eight-week periods, from the eighth week onwards after the start of feeding. Eight ejaculates were collected from each boar, totaling 96 samples. The addition of EELO to the diets of boars caused an increase in sperm viability (p < 0.001), semen volume (310 mL versus 216 mL, p < 0.001) and sperm concentration (331 versus 216 million per mL, p < 0.001). Furthermore, in the experimental animals, there was a decrease in the percentage of spermatozoa exhibiting DNA fragmentation. The experimental boars also showed an increase in the percentage of gametes without apoptosis and capacitation and an increase in the percentage of viable spermatozoa not showing lipid peroxidation membranes. Consequently, EELO nutritional supplementation resulted in the improved quality of the fresh semen of boars.
Age Rather Than Supplementation with Oat β-Glucan Influences Development of the Intestinal Microbiota and SCFA Concentrations in Suckling Piglets
Authors: Arapovic Lidija , Huang Yi , Manell Elin , Verbeek Else , Keeling Linda , Sun Li , Landberg Rikard , Lundh Torbjörn , Lindberg Jan Erik , Dicksved Johan
Published: April 2023

The effects of early supplementation with oat β-glucan during the suckling period on piglet gut microbiota composition, concentrations of short-chain fatty acids, and gut physiological markers were assessed. Fifty piglets from five litters, balanced for sex and birth weight, were divided within litters into two treatment groups: β-glucan and control. Piglets in the β-glucan group received the supplement three times/week from day 7 of age until weaning. Rectal swab samples were collected from 10 piglets per treatment group (balanced across litters) from week 1 to week 4, and plasma samples were collected at 1, 3, and 4 weeks of age. Additional samples of intestinal tissues and jugular and portal vein plasma were collected from 10 animals at weaning (one per treatment group and litter). The concentrations of short-chain fatty acids in plasma and the microbiota composition in rectal swabs were mainly influenced by piglet age, rather than the supplement. There were significant differences in microbiota composition between litters and several correlations between concentrations of short-chain fatty acids in plasma and specific microbial taxa in rectal swabs. Overall, β-glucan supplementation did not have any clear impact on the gut environment in suckling piglets, whereas a clear age-related pattern emerged.
Complex Networks Analyses of Antibiofilm Peptides: An Emerging Tool for Next-Generation Antimicrobials’ Discovery
Authors: Agüero-Chapin Guillermin , Antunes Agostinho , Mora José R. , Pérez Noel , Contreras-Torres Ernesto , Valdes-Martini José R. , Martinez-Rios Felix , Zambrano Cesar H. , Marrero-Ponce Yovani
Published: April 2023

Microbial biofilms cause several environmental and industrial issues, even affecting human health. Although they have long represented a threat due to their resistance to antibiotics, there are currently no approved antibiofilm agents for clinical treatments. The multi-functionality of antimicrobial peptides (AMPs), including their antibiofilm activity and their potential to target multiple microbes, has motivated the synthesis of AMPs and their relatives for developing antibiofilm agents for clinical purposes. Antibiofilm peptides (ABFPs) have been organized in databases that have allowed the building of prediction tools which have assisted in the discovery/design of new antibiofilm agents. However, the complex network approach has not yet been explored as an assistant tool for this aim. Herein, a kind of similarity network called the half-space proximal network (HSPN) is applied to represent/analyze the chemical space of ABFPs, aiming to identify privileged scaffolds for the development of next-generation antimicrobials that are able to target both planktonic and biofilm microbial forms. Such analyses also considered the metadata associated with the ABFPs, such as origin, other activities, targets, etc., in which the relationships were projected by multilayer networks called metadata networks (METNs). From the complex networks’ mining, a reduced but informative set of 66 ABFPs was extracted, representing the original antibiofilm space. This subset contained the most central to atypical ABFPs, some of them having the desired properties for developing next-generation antimicrobials. Therefore, this subset is advisable for assisting the search for/design of both new antibiofilms and antimicrobial agents. The provided ABFP motifs list, discovered within the HSPN communities, is also useful for the same purpose.
Novel Mutations Conferring Amoxicillin Resistance in Helicobacter pylori in South Korea
Authors: Park Soon Young , Lee Eun Hwa , Kim Dokyun , Song Young Goo , Jeong Su Jin
Published: April 2023

Helicobacter pylori is the primary causative agent of gastritis, gastric ulcers, duodenal ulcers, gastric cancer, and peripheral B-cell lymphoma. H. pylori eradication often fails due to elevated antibiotic resistance. However, no previous studies have thoroughly examined amoxicillin resistance. Here, the objective was to identify clinical strains of H. pylori with amoxicillin resistance and to analyze single-nucleotide polymorphisms (SNPs) associated with amoxicillin resistance. From March 2015 to June 2019, genotypic and phenotypic amoxicillin resistance was analyzed using an E-test and whole-genome sequencing (WGS). Analysis of 368 clinical strains confirmed amoxicillin resistance in 31 strains (resistance rate of 8.7%). The genomes were extracted from nine resistant (<0.125 mg/L) strains, and WGS was performed for genetic analysis. WGS analysis identified SNPs present in pbp1a, pbp2, nhaC, hofH, hofC, and hefC in all nine isolates. Some of these genes may be related to amoxicillin resistance. A total of six SNPs (A69V, V374L, S414R, T503I, A592D, and R435Q) were identified in PBP2 of H-8, the most resistant strain. We predict that these six SNPs are associated with high amoxicillin resistance. Amoxicillin resistance should be considered in the clinical setting for the treatment failure of H. pylori eradication.
Editorial for the Special Issue: “Current Technique for Antibiotic Susceptibility Test: Advantages and Limitations; Need for Next-Generation Test”
Authors: Nicolai Eleonora
Published: April 2023

The overuse or misuse of antibiotics, either when recommended by physicians or administered through self-medication at the time of infection, has caused drug-resistant pathogens to become a major healthcare issue, with millions of reported cases every year [...]
A Century of Clinical Use of Phages: A Literature Review
Authors: Diallo Kevin , Dublanchet Alain
Published: April 2023

Growing antibiotic resistance and the broken antibiotic market have renewed interest in the use of phages, a century-old therapy that fell into oblivion in the West after two decades of promising results. This literature review with a particular focus on French literature aims to complement current scientific databases with medical and non-medical publications on the clinical use of phages. While several cases of successful treatment with phages have been reported, prospective randomized clinical trials are needed to confirm the efficacy of this therapy.
Local Antibiotic Delivery Options in Prosthetic Joint Infection
Authors: Steadman William , Chapman Paul R. , Schuetz Michael , Schmutz Beat , Trampuz Andrej , Tetsworth Kevin
Published: April 2023

Prosthetic Joint Infection (PJI) causes significant morbidity and mortality for patients globally. Delivery of antibiotics to the site of infection has potential to improve the treatment outcomes and enhance biofilm eradication. These antibiotics can be delivered using an intra-articular catheter or combined with a carrier substance to enhance pharmacokinetic properties. Carrier options include non-resorbable polymethylmethacrylate (PMMA) bone cement and resorbable calcium sulphate, hydroxyapatite, bioactive glass, and hydrogels. PMMA allows for creation of structural spacers used in multi-stage revision procedures, however it requires subsequent removal and antibiotic compatibility and the levels delivered are variable. Calcium sulphate is the most researched resorbable carrier in PJI, but is associated with wound leakage and hypercalcaemia, and clinical evidence for its effectiveness remains at the early stage. Hydrogels provide a versatile combability with antibiotics and adjustable elution profiles, but clinical usage is currently limited. Novel anti-biofilm therapies include bacteriophages which have been used successfully in small case series.
Antibiofilm Effect of Biogenic Silver Nanoparticles Combined with Oregano Derivatives against Carbapenem-Resistant Klebsiella pneumoniae
Authors: Scandorieiro Sara , Teixeira Franciele Maira M. B. , Nogueira Mara C. L. , Panagio Luciano A. , de Oliveira Admilton G. , Durán Nelson , Nakazato Gerson , Kobayashi Renata K. T.
Published: April 2023

Resistant bacteria may kill more people than COVID-19, so the development of new antibacterials is essential, especially against microbial biofilms that are reservoirs of resistant cells. Silver nanoparticles (bioAgNP), biogenically synthesized using Fusarium oxysporum, combined with oregano derivatives, present a strategic antibacterial mechanism and prevent the emergence of resistance against planktonic microorganisms. Antibiofilm activity of four binary combinations was tested against enteroaggregative Escherichia coli (EAEC) and Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC): oregano essential oil (OEO) plus bioAgNP, carvacrol (Car) plus bioAgNP, thymol (Thy) plus bioAgNP, and Car plus Thy. The antibiofilm effect was accessed using crystal violet, MTT, scanning electron microscopy, and Chromobacterium violaceum anti-quorum-sensing assays. All binary combinations acted against preformed biofilm and prevented its formation; they showed improved antibiofilm activity compared to antimicrobials individually by reducing sessile minimal inhibitory concentration up to 87.5% or further decreasing biofilm metabolic activity and total biomass. Thy plus bioAgNP extensively inhibited the growth of biofilm in polystyrene and glass surfaces, disrupted three-dimensional biofilm structure, and quorum-sensing inhibition may be involved in its antibiofilm activity. For the first time, it is shown that bioAgNP combined with oregano has antibiofilm effect against bacteria for which antimicrobials are urgently needed, such as KPC.
Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Acquisition and Colonization at a Korean Hospital over 1 Year
Authors: Kim Hye-Jin , Hyun Jung-Hee , Jeong Hyo-Seon , Lee Yeon-Kyeng
Published: April 2023

Background: Carbapenemase-producing Enterobacteriaceae (CPE) are known to be primarily responsible for the increasing spread of carbapenem-resistant Enterobacteriaceae and have therefore been targeted for preventing transmission and appropriate treatment. This study aimed to describe the clinical and epidemiological characteristics and risk factors of CPE infection in terms of acquisition and colonization. Methods: We examined patients’ hospital data, including active screening on patients’ admission and in intensive care units (ICUs). We identified risk factors for CPE acquisition by comparing the clinical and epidemiological data of CPE-positive patients between colonization and acquisition groups. Results: A total of 77 CPE patients were included (51 colonized and 26 acquired). The most frequent Enterobacteriaceae species was Klebsiella pneumoniae. Among CPE-colonized patients, 80.4% had a hospitalization history within 3 months. CPE acquisition was significantly associated with treatment in an ICU [adjusted odds ratio (aOR): 46.72, 95% confidence interval (CI): 5.08–430.09] and holding a gastrointestinal tube (aOR: 12.70, 95% CI: 2.61–61.84). Conclusions: CPE acquisition was significantly associated with ICU stay, open wounds, holding catheters or tubes, and antibiotic treatment. Active CPE screening should be implemented on admission and periodically for high-risk patients.
Bioavailability of Organosulfur Compounds after the Ingestion of Black Garlic by Healthy Humans
Authors: Moreno-Ortega Alicia , Pereira-Caro Gema , Ludwig Iziar A. , Motilva María-José , Moreno-Rojas José Manuel
Published: April 2023

The consumption of black garlic has been related to a decreased risk of many human diseases due to the presence of phytochemicals such as organosulfur compounds (OSCs). However, information on the metabolization of these compounds in humans is limited. By means of ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS), this study aims to determine the OSCs and their metabolites excreted in urine 24 h after an acute intake of 20 g of black garlic by healthy humans. Thirty-three OSCs were identified and quantified, methiin (17,954 ± 6040 nmol), isoalliin (15,001 ± 9241 nmol), S-(2-carboxypropyl)-L-cysteine (8804 ± 7220 nmol) and S-propyl-L-cysteine (deoxypropiin) (7035 ± 1392 nmol) being the main ones. Also detected were the metabolites N-acetyl-S-allyl-L-cysteine (NASAC), N-acetyl-S-allyl-L-cysteine sulfoxide (NASACS) and N-acetyl-S-(2-carboxypropyl)-L-cysteine (NACPC), derived from S-allyl-L-cysteine (SAC), alliin and S-(2-carboxypropyl)-L-cysteine, respectively. These compounds are potentially N-acetylated in the liver and kidney. The total excretion of OSCs 24 h after the ingestion of black garlic was 64,312 ± 26,584 nmol. A tentative metabolic pathway has been proposed for OSCs in humans.
Agomelatine, a Melatonin-Derived Drug, as a New Strategy for the Treatment of Colorectal Cancer
Authors: Moreno-SanJuan Sara , Puentes-Pardo Jose D. , Casado Jorge , Escudero-Feliu Julia , Khaldy Huda , Arnedo Javier , Carazo Ángel , León Josefa
Published: April 2023

The potential use of agomelatine as an alternative treatment for colorectal cancer is evaluated in this work. The effect of agomelatine was studied in an in vitro model using two cell lines with different p53 statuses (HCT-116, wild-type p53, and HCT-116 p53 null) and an in vivo xenograft model. The inhibitory effects of agomelatine and melatonin were stronger in the cells harboring the wild-type p53, although in both cell lines, the effect of agomelatine was greater than that of the melatonin. In vivo, only agomelatine was able to reduce the volumes of tumors generated by the HCT-116-p53-null cells. Both treatments induced changes in the rhythmicity of the circadian-clock genes in vitro, albeit with some differences. Agomelatine and melatonin regulated the rhythmicity of Per1-3, Cry1, Sirt1, and Prx1 in the HCT-116 cells. In these cells, agomelatine also regulated Bmal1 and Nr1d2, while melatonin changed the rhythmicity of Clock. In the HCT-116-p53-null cells, agomelatine regulated Per1-3, Cry1, Clock, Nr1d2, Sirt1, and Prx1; however, melatonin only induced changes in Clock, Bmal1, and Sirt1. The differences found in the regulation of the clock genes may explain the greater oncostatic effect of agomelatine in CRC.
Associations between Oxidant/Antioxidant Status and Circulating Adipokines in Non-Obese Children with Prader–Willi Syndrome
Authors: Gajewska Joanna , Ambroszkiewicz Jadwiga , Szamotulska Katarzyna , Rowicka Grażyna , Strucińska Małgorzata , Klemarczyk Witold , Chełchowska Magdalena
Published: April 2023

Oxidative stress is implicated in the pathophysiology of Prader–Willi syndrome (PWS), but there are no data on these disorders in non-obese children with PWS. Therefore, the presented study examined total oxidant capacity (TOC), total antioxidant capacity (TAC), the oxidative stress index (OSI), and adipokine levels in 22 non-obese children with PWS during dietary intervention and growth hormone treatment compared with 25 non-obese healthy children. Serum concentrations of TOC, TAC, nesfatin-1, leptin, hepcidin, ferroportin, and ferritin were determined using immunoenzymatic methods. We found that TOC concentrations were higher by 50% (p = 0.006) in patients with PWS than in healthy children, but no significant differences in TAC concentrations were observed between these groups. The OSI was higher in children with PWS than in the controls (p = 0.002). We found positive associations between TOC values and the percentage of the Estimated Energy Requirement, body mass index (BMI) Z-score, percentage of fat mass, and leptin, nesfatin-1, and hepcidin concentrations in patients with PWS. A positive association was also found between the OSI and nesfatin-1 levels. These observations suggest that higher daily energy intake and weight gain may be accompanied by an increasing prooxidant state in these patients. Adipokines such as leptin, nesfatin-1, or hepcidin may also play a role in the prooxidant state in non-obese children with PWS.
Role of Advanced Glycation End-Products and Oxidative Stress in Type-2-Diabetes-Induced Bone Fragility and Implications on Fracture Risk Stratification
Authors: Cavati Guido , Pirrotta Filippo , Merlotti Daniela , Ceccarelli Elena , Calabrese Marco , Gennari Luigi , Mingiano Christian
Published: April 2023

Type 2 diabetes (T2D) and osteoporosis (OP) are major causes of morbidity and mortality that have arelevant health and economic burden. Recent epidemiological evidence suggests that both of these disorders are often associated with each other and that T2D patients have an increased risk of fracture, making bone an additional target of diabetes. As occurs for other diabetic complications, the increased accumulation of advanced glycation end-products (AGEs) and oxidative stress represent the major mechanisms explaining bone fragility in T2D. Both of these conditions directly and indirectly (through the promotion of microvascular complications) impair the structural ductility of bone and negatively affect bone turnover, leading to impaired bone quality, rather than decreased bone density. This makes diabetes-induced bone fragility remarkably different from other forms of OP and represents a major challenge for fracture risk stratification, since either the measurement of BMD or the use of common diagnostic algorithms for OP have a poor predictive value. We review and discuss the role of AGEs and oxidative stress on the pathophysiology of bone fragility in T2D, providing some indications on how to improve fracture risk prediction in T2D patients.
The Inhibitory Effects of Hydroxytyrosol, α-Tocopherol and Ascorbyl Palmitate on Lipid Peroxidation in Deep-Fat Fried Seafood
Authors: Theah Audrey Yue Vern , Akanbi Taiwo O.
Published: April 2023

This study aimed to investigate the inhibitory effects of hydroxytyrosol, α-tocopherol and ascorbyl palmitate on lipid peroxidation in squid, hoki and prawn during deep-fat frying and refrigerated storage. Fatty acid analysis using gas chromatography (GC) showed that the seafood had a high omega-3 polyunsaturated fatty acid (n-3 PUFAs) content, including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). The total content of n-3 fatty acids in their lipids was 46% (squid), 36% (hoki) and 33% (prawn), although they all had low lipid contents. The oxidation stability test results showed that deep-fat frying significantly increased the peroxide value (POV), p-anisidine value (p-AV) and the value of thiobarbituric acid reactive substances (TBARS) in squid, hoki and prawn lipids. Meanwhile, antioxidants delayed the lipid oxidation in fried seafood and sunflower oil (SFO) used for frying, albeit in different ways. The least effective of all the antioxidants was α-tocopherol, as the POV, p-AV and TBARS values obtained with this antioxidant were significantly higher. Ascorbyl palmitate was better than α-tocopherol but was not as effective as hydroxytyrosol in suppressing lipid oxidation in the frying medium (SFO) and in the seafood. However, unlike the ascorbyl palmitate-treated oil, hydroxytyrosol-treated oil could not be used for multiple deep-fat frying of seafood. Hydroxytyrosol appeared to be absorbed in the seafood during multiple frying, thus leaving a low concentration in the SFO and making it susceptible to oxidation.
Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism
Authors: Rezgui Raja , Walia Ruhi , Sharma Jyoti , Sidhu Dwinder , Alshagadali Khalid , Ray Chaudhuri Saumya , Saeed Amir , Dey Priyankar
Published: April 2023

Centering around the concept that metabolites from the gut commensals can exert metabolic health benefits along the gut–liver axis, we tested whether the cell-free global metabolome of probiotic bacteria can exert hepatoprotective benefits against H2O2-induced oxidative stress. Cell-free global metabolites of Lactobacillus plantarum (LPM) were isolated and untargeted metabolomics was performed. The free radical scavenging potentials of LPM were measured. The cytoprotective effects of LPM were tested on HepG2 cells. A total of 66 diverse metabolites were identified in LPM, among which saturated fatty acids, amino acids and dicarboxylic acids were highly enriched. LPM attenuated cell damage, lipid peroxidation and the levels of intracellular cytoprotective enzymes in H2O2-treated cells. LPM also attenuated H2O2-induced increased expressions of TNF-α and IL-6. However, the cytoprotective effects of LPM were diminished in cells that were pretreated with a pharmacological inhibitor of Nrf2. Our data collectively indicate that LPM can significantly attenuate oxidative damage to HepG2 cells. However, the cytoprotective effects of LPM likely depend on an Nrf2-dependent mechanism.
Nrf2 Protein Serum Concentration in Human CKD Shows a Biphasic Behavior
Authors: Rasmussen Marianne , Hansen Kristian Horsman , Scholze Alexandra
Published: April 2023

Oxidative stress contributes to the progression of chronic kidney disease (CKD) and CKD-related mortality. The nuclear factor erythroid 2-related factor 2 (Nrf2) is essential in the regulation of cellular redox status, and Nrf2-activating therapies are under evaluation in several chronic diseases, including CKD. It is therefore inevitable to understand how Nrf2 behaves in advancing CKD. We analyzed Nrf2 protein concentrations in patients with varying extents of CKD but without renal replacement therapy, and in healthy subjects. Compared to healthy controls, Nrf2 protein was upregulated in mild to moderate kidney function impairment (G1–3). Within the CKD population, we found a significant positive correlation between Nrf2 protein concentration and kidney function (estimated glomerular filtration rate). In severe kidney function impairment (G4,5), Nrf2 protein was reduced compared to mild to moderate kidney function impairment. We conclude that Nrf2 protein concentration in severe kidney function impairment is reduced relative to the mild to moderate kidney function impairment where increased Nrf2 protein concentrations prevail. With respect to the implementation of Nrf2 targeted therapies, it will be necessary to explore in which population of patients with CKD such therapies are able to effectively add to the endogenous Nrf2 activity.
Multimodal Fusion with Dual-Attention Based on Textual Double-Embedding Networks for Rumor Detection
Authors: Han Huawei , Ke Zunwang , Nie Xiangyang , Dai Li , Slamu Wushour
Published: April 2023

Rumors may bring a negative impact on social life, and compared with pure textual rumors, online rumors with multiple modalities at the same time are more likely to mislead users and spread, so multimodal rumor detection cannot be ignored. Current detection methods for multimodal rumors do not focus on the fusion of text and picture-region object features, so we propose a multimodal fusion neural network TDEDA (dual-attention based on textual double embedding) applied to rumor detection, which performs a high-level information interaction at the text–image object level and captures visual features associated with keywords using an attention mechanism. In this way, we explored the ability to enhance feature representation with assistance from different modalities in rumor detection, as well as to capture the correlations of the dense interaction between images and text. We conducted comparative experiments on two multimodal rumor detection datasets. The experimental results showed that TDEDA could reasonably handle multimodal information and thus improve the accuracy of rumor detection compared with currently relevant multimodal rumor detection methods.
Multirobot Task Planning Method Based on the Energy Penalty Strategy
Authors: Liang Lidong , Zhu Liangheng , Jia Wenyou , Cheng Xiaoliang
Published: April 2023

In multirobot task planning, the goal is to meet the multi-objective requirements of the optimal and balanced energy consumption of robots. Thus, this paper introduces the energy penalty strategy into the GA (genetic algorithm) to achieve the optimization of the task planning of multiple robots in different operation scenarios. First, the algorithm model is established, after which the objective function is constructed by taking the energy excess of the relative average energy consumption of each robot as the penalty energy, along with the total energy consumption of multiple robots. In the genetic operation, two-segment chromosome coding is used to realize the iterative optimization of the number and task sequences of robots through diversified cross and mutation operators. Then, in the task scenario with obstacles, the A* (A-Star) algorithm and GA are used to plan the optimal obstacle avoidance path and to realize the secondary optimization of the robot task sequence without changing the number of tasks. During optimization, the energy penalty strategy imposes punishment on the objective function through the size of the penalty energy, enabling the robot energy consumption to reach an equilibrium state by maintaining the total energy consumption at the minimum. Finally, the MATLAB platform is used to conduct the simulation experiments to compare with basic genetic algorithms and penalty function algorithms, after which the optimal allocation scheme and energy consumption iteration of the algorithm are analyzed under different robot numbers, task numbers, and task scenarios, and the simulation results include robot task sequences, total energy consumption, average energy consumption, and standard deviation of energy consumption.
Physical and Antioxidant Properties of Innovative Gluten-Free Bread with the Addition of Hemp Inflorescence
Authors: Pecyna Anna , Buczaj Agnieszka , Różyło Renata , Kobus Zbigniew
Published: April 2023

Hemp inflorescences from byproducts have been proposed as an addition to gluten-free rice bread. The scope of the research was to bake a control loaf of bread as well as bread loaves containing 1%, 2%, 3%, 4%, and 5% dried and crushed hemp inflorescence (HI). The loaves of bread were evaluated in terms of their physical and sensory properties, polyphenol and flavonoid contents, and DPPH and FRAP antioxidant activities. The study’s findings revealed that the addition of HI influenced changes in the physical properties of the bread loaves, such as increased specific volume, decreased bread hardness, increased elasticity, and chewiness of the breadcrumb, especially when the additive concentrations were greater than 3%. The addition of HI significantly increased the total amount of polyphenols, flavonoids, and antioxidant activity in the bread. The sensory evaluation revealed that gluten-free bread can be produced with a maximum of 2% HI without affecting its taste and aroma.