bluuebunny/crossref_metadata_2025 huggingface dataset:
Human Papillomavirus E6/E7 Expression in Preeclampsia-Affected Placentae
Authors: Reily-Bell Ashley L. , Fisher Amanda , Harrison Bryony , Bowie Sara , Ray Sankalita , Hawkes Mary , Wise Lyn M. , Fukuzawa Ryuji , Macaulay Erin C. , Devenish Celia J. , Hung Noelyn A. , Slatter Tania L.
Published: March 2020

Whether HPV is causative of pregnancy complications is uncertain. E6 and E7 affect functions underling preeclampsia (PET) in cultured trophoblasts, but whether E6 and E7 is produced in the placenta is uncertain. Here, we investigated whether E6/E7 was expressed in the placentae from pregnancies with PET, other pregnancy complications (fetal growth restriction (FGR) and diabetes mellitus), and uncomplicated pregnancies. Placental tissues collected from two geographical locations were subjected to RNAscope analyses of high- and low- risk E6/E7, and individual HPV types identified using an HPV array. High-risk E6/E7 expression was increased in both PET cohorts, (81% and 86% of patients positive for high-risk HPV DNA compared to 13% of control patients). Various HPV types were identified. Although HPV 18 was the most frequent in all cohorts, the majority of individuals had multiple HPV types (55% of the PET compared to 25% of the control cohort). Further evidence that E6 and E7 is present early when placental pathology underlying preeclampsia is established, is provided with the finding of high-risk E6/E7 in the first-trimester placenta anchoring trophoblast. In conclusion, E6/E7 expression and multiple HPV types were frequent in placentae from preeclampsia-complicated pregnancies.
Evolutionary Trajectory for the Emergence of Novel Coronavirus SARS-CoV-2
Authors: Rehman Saif ur , Shafique Laiba , Ihsan Awais , Liu Qingyou
Published: March 2020

Over the last two decades, the world experienced three outbreaks of coronaviruses with elevated morbidity rates. Currently, the global community is facing emerging virus SARS-CoV-2 belonging to Betacoronavirus, which appears to be more transmissible but less deadly than SARS-CoV. The current study aimed to track the evolutionary ancestors and different evolutionary strategies that were genetically adapted by SARS-CoV-2. Our whole-genome analysis revealed that SARS-CoV-2 was the descendant of Bat SARS/SARS-like CoVs and bats served as a natural reservoir. SARS-CoV-2 used mutations and recombination as crucial strategies in different genomic regions including the envelop, membrane, nucleocapsid, and spike glycoproteins to become a novel infectious agent. We confirmed that mutations in different genomic regions of SARS-CoV-2 have specific influence on virus reproductive adaptability, allowing for genotype adjustment and adaptations in rapidly changing environments. Moreover, for the first time we identified nine putative recombination patterns in SARS-CoV-2, which encompass spike glycoprotein, RdRp, helicase and ORF3a. Six recombination regions were spotted in the S gene and are undoubtedly important for evolutionary survival, meanwhile this permitted the virus to modify superficial antigenicity to find a way from immune reconnaissance in animals and adapt to a human host. With these combined natural selected strategies, SARS-CoV-2 emerged as a novel virus in human society.
Synthesis and Characterization of Polyfumarateurethane Nanoparticles for Sustained Release of Bupivacaine
Authors: Park Soo-Yong , Kang Jiin , Yoon Ji-Young , Chung Ildoo
Published: March 2020

Biodegradable polyfumarateurethane (PFU) for use as a bupivacaine delivery vehicle, synthesized using di-(2-hydroxypropyl fumarate) (DHPF), polyethylene glycol (PEG) and 1,6-hexamethylene diisocyanate (HMDI), was designed to be degradable through the hydrolysis and enzymatic degradation of the ester bonds in its polymer backbone. Using a water-in-oil-in-water double emulsion techniques, nanoparticles encapsulating water or fluorescein isothiocyanate (FITC) were fabricated to avoid the immune system owing to the presence of PEG on their surface. The morphologies of these nanoparticles were characterized by DLS, TEM, FE-SEM, and fluorescent microscopies. The present study explored the encapsulation, loading efficiency and in vitro drug release of bupivacaine encapsulated with biodegradable PFU nanoparticles for the treatment of local anesthesia. Various concentrations of bupivacaine were encapsulated into nanoparticles and their encapsulation efficiencies and drug loading were investigated. Encapsulation efficiency was highest when 2.5% bupivacaine was encapsulated. Drug release behavior from the bupivacaine-loaded PFU nanoparticles followed a sustained release profile.
Mechanochemical Formation of Racemic Praziquantel Hemihydrate with Improved Biopharmaceutical Properties
Authors: Zanolla Debora , Hasa Dritan , Arhangelskis Mihails , Schneider-Rauber Gabriela , Chierotti Michele R. , Keiser Jennifer , Voinovich Dario , Jones William , Perissutti Beatrice
Published: March 2020

Praziquantel (PZQ) is the first-line drug used against schistosomiasis, one of the most common parasitic diseases in the world. A series of crystalline structures including two new polymorphs of the pure drug and a series of cocrystals of PZQ have been discovered and deposited in the Cambridge Structural Database (CSD). This work adds to the list of multicomponent forms of PZQ a relevant example of a racemic hemihydrate (PZQ-HH), obtainable from commercial PZQ (polymorphic Form A) through mechanochemistry. Noteworthy, the formation of the new hemihydrate strongly depends on the initial polymorphic form of PZQ and on the experimental conditions used. The new PZQ-HH has been fully characterized by means of HPLC, Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), Hot-Stage Microscopy (SEM), Powder X-Ray Diffraction (PXRD), Scanning Electron Microscopy (SEM), FT-IR, polarimetry, solid-state NMR (SS-NMR), solubility and intrinsic dissolution rate (IDR), and in vitro tests on Schistosoma mansoni adults. The crystal structure was solved from the powder X-ray diffraction pattern and validated by periodic-DFT calculations. The new bioactive hemihydrate was physically stable for three months and showed peculiar biopharmaceutical features including enhanced solubility and a double intrinsic dissolution rate in water in comparison to the commercially available PZQ Form A.
Optimal Connection for Tiotropium SMI Delivery through Mechanical Ventilation: An In Vitro Study
Authors: Fang Tien-Pei , Chen Yu-Ju , Yang Tsung-Ming , Wang Szu-Hu , Hung Ming-Szu , Chiu Shu-Hua , Li Hsin-Hsien , Fink James B. , Lin Hui-Ling
Published: March 2020

We aimed to quantify Soft Mist Inhalers (SMI) delivery to spontaneous breathing model and compare with different adapters via endotracheal tube during mechanical ventilation or by manual resuscitation. A tiotropium SMI was used with a commercial in-line adapter and a T-adapter placed between the Y-adapter and the inspiratory limb of the ventilator circuit during mechanical ventilation. The SMI was actuated at the beginning of inspiration and expiration. In separate experiments, a manual resuscitator with T-adapter was attached to endotracheal tube, collecting filter, and a passive test lung. Drug was eluted from collecting filters with salt-based solvent and analyzed using high-performance liquid chromatography. Results showed the percent of SMI label dose inhaled was 3-fold higher with the commercial in-line adapter with actuation during expiration than when synchronized with inspiration. SMI with T-adapter delivery via ventilator was similar to inhalation (1.20%) or exhalation (1.02%), and both had lower delivery dose than with manual resuscitator (2.80%; p = 0.01). The inhaled dose via endotracheal tube was much lower than inhaled dose with spontaneous breathing (22.08%). In conclusion, the inhaled dose with the commercial adapter was higher with SMI actuated during expiration, but still far less than reported spontaneous inhaled dose.
Segmental-Dependent Solubility and Permeability as Key Factors Guiding Controlled Release Drug Product Development
Authors: Markovic Milica , Zur Moran , Fine-Shamir Noa , Haimov Ester , González-Álvarez Isabel , Dahan Arik
Published: March 2020

The main factors influencing the absorption of orally administered drugs are solubility and permeability, which are location-dependent and may vary along the gastrointestinal tract (GIT). The purpose of this work was to investigate segmental-dependent intestinal absorption and its role in controlled-release (CR) drug product development. The solubility/dissolution and permeability of carvedilol (vs. metoprolol) were thoroughly studied, in vitro/in vivo (Octanol-buffer distribution coefficients (Log D), parallel artificial membrane permeability assay (PAMPA), rat intestinal perfusion), focusing on location-dependent effects. Carvedilol exhibits changing solubility in different conditions throughout the GIT, attributable to its zwitterionic nature. A biorelevant pH-dilution dissolution study for carvedilol immediate release (IR) vs. CR scenario elucidates that while the IR dose (25 mg) may dissolve in the GIT luminal conditions, higher doses used in CR products would precipitate if administered at once, highlighting the advantage of CR from the solubility/dissolution point of view. Likewise, segmental-dependent permeability was evident, with higher permeability of carvedilol vs. the low/high Peff marker metoprolol throughout the GIT, confirming it as a biopharmaceutical classification system (BCS) class II drug. Theoretical analysis of relevant physicochemical properties confirmed these results as well. A CR product may shift the carvedilol’s solubility behavior from class II to I since only a small dose portion needs to be solubilized at a given time point. The permeability of carvedilol surpasses the threshold of metoprolol jejunal permeability throughout the entire GIT, including the colon, establishing it as a suitable candidate for CR product development. Altogether, this work may serve as an analysis model in the decision process of CR formulation development and may increase our biopharmaceutical understanding of a successful CR drug product.
Mutations of OsPLDa1 Increase Lysophospholipid Content and Enhance Cooking and Eating Quality in Rice
Authors: Khan Muhammad Saad Shoaib , Basnet Rasbin , Ahmed Sulaiman , Bao Jinsong , Shu Qingyao
Published: March 2020

Phospholipids belong to a significant class of lipids and comprise ~10% of total lipids in rice grains. Lysophospholipid (LPL) is derived from the hydrolysis of phospholipids and plays an important role in rice grain quality. Our previous study demonstrated that mutations in a phospholipase D gene (OsPLDα1) significantly altered lipid metabolites and reduced phytic acid content. In the present study, the effect of two ospldα1 mutations on LPL and other physicochemical prosperities of brown rice was further investigated, with the aim of assessing the overall importance of ospldα1 mutations in rice grain quality. Metabolite profiling revealed a ~15% increase in LPL level in both ospldα1 mutants as compared with their wild-type (WT) parent. Both ospldα1 mutations significantly lowered the apparent amylose content in brown rice flour (~1.9%) and altered viscosity profiles with significantly increased breakdown (+12.4%) and significantly reduced setback viscosity (−6.2%). Moreover, both ospldα1 mutations significantly lowered the gelatinization onset, peak temperature and retrogradation percentage of brown rice flour. This study demonstrated that OsPLDα1 plays a crucial role in rice grain quality and its mutation could, in general, improve the cooking and eating quality and nourishment of brown rice.
Ecological and Landscape Factors Affecting the Spread of European Mistletoe (Viscum album L.) in Urban Areas (A Case Study of the Kaliningrad City, Russia)
Authors: Skrypnik Liubov , Maslennikov Pavel , Feduraev Pavel , Pungin Artem , Belov Nikolay
Published: March 2020

Green spaces are very important for an urban environment. Trees in cities develop under more stressful conditions and are, therefore, more susceptible to parasite including mistletoe infestation. The aim of this study was to investigate the ecological, microclimatic, and landscape factors causing the spread of European mistletoe (Viscum album L.) in urban conditions. The most numerous hosts of mistletoe were Tilia cordata (24.4%), Acer platanoides (22.7%), and Populus nigra (16.7%). On average, there were more than 10 mistletoe bushes per tree. The mass mistletoe infestations (more than 50 bushes per the tree) were detected for Populus × berolinensis, Populus nigra, and Acer saccharinum. The largest number of infected trees was detected in the green zone (city parks), historical housing estates, and green zone along water bodies. Based on the results of principal component analysis (PCA), the main factors causing the spread of mistletoe on the urban territories are trees’ age and relative air humidity. The factors reflecting environmental pollution (the content of heavy metals in the soil and the concentration of nitrogen dioxide in the air) did not statistically affect the mistletoe distribution in the study area. However, this result may be due to the heterogeneity of other parameters in the studied areas. Therefore, additional research is required to more accurately interpret the data on the relationship between environmental pollutions and distribution of mistletoe infestation of trees in urban areas.
The Effect of Recombinant Tags on Citrus paradisi Flavonol-Specific 3-O Glucosyltransferase Activity
Authors: Birchfield Aaron S. , McIntosh Cecilia A.
Published: March 2020

Recombinant tags are used extensively in protein expression systems to allow purification through IMAC (Immobilized Metal Affinity Chromatography), identification through Western blot, and to facilitate crystal formation for structural analysis. While widely used, their role in enzyme characterization has raised concerns with respect to potential impact on activity. In this study, a flavonol-specific 3-O glucosyltransferase (Cp3GT) from grapefruit (Citrus paradisi) was expressed in Pichia pastoris, and was assayed in its untagged form and with a C-terminal c-myc/6x His tag under various conditions to determine the effect of tags. Prior characterization of pH optima for Cp3GT obtained through expression in Escherichia coli, containing an N-terminal thioredoxin/6x His tag, indicated an optimal pH of 7–7.5, which is indicative of a normal physiological pH and agrees with other glucosyltransferase (GT) pH optima. However, characterization of Cp3GT expressed using P. pastoris with a C-terminal c-myc-6x His tag showed a higher optimal pH of 8.5–9. This suggests a possible tag effect or an effect related to physiological differences between the cell expression systems. Results testing recombinant Cp3GT expressed in Pichia with and without C-terminal tags showed a possible tag effect with regard to substrate preference and interactions with metals, but no apparent effect on enzymatic kinetics or pH optima.
Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
Authors: Vollár Martin , Feigl Gábor , Oláh Dóra , Horváth Attila , Molnár Árpád , Kúsz Norbert , Ördög Attila , Csupor Dezső , Kolbert Zsuzsanna
Published: March 2020

Similar to animals, it has recently been proven that nitro-fatty acids such as nitro-linolenic acid and nitro-oleic acid (NO2-OA) have relevant physiological roles as signalling molecules also in plants. Although NO2-OA is of great therapeutic importance, its presence in plants as a free fatty acid has not been observed so far. Since Brassica napus (oilseed rape) is a crop with high oleic acid content, the abundance of NO2-OA in its tissues can be assumed. Therefore, we quantified NO2-OA in B. napus seeds and differently developed seedlings. In all samples, NO2-OA was detectable at nanomolar concentrations. The seeds showed the highest NO2-OA content, which decreased during germination. In contrast, nitric oxide (•NO) levels increased in the early stages of germination and seedling growth. Exogenous NO2-OA treatment (100 µM, 24 h) of Brassica seeds resulted in significantly increased •NO level and induced germination capacity compared to untreated seeds. The results of in vitro approaches (4-Amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM) fluorescence, •NO-sensitive electrode) supported the •NO liberating capacity of NO2-OA. We observed for the first time that Brassica seeds and seedlings contain free NO2-OA which may be involved in germination as an •NO donor as suggested both by the results of exogenous NO2-OA treatment of seeds and in vitro approaches. Due to their high NO2-OA content, Brassica sprouts can be considered as a good source of dietary NO2-OA intake.
Preparation and Application of Light-Colored Lignin Nanoparticles for Broad-Spectrum Sunscreens
Authors: Lee Sang Cheon , Yoo Eunjin , Lee Sang Hyun , Won Keehoon
Published: March 2020

Recently, natural sun blockers have been drawing considerable attention because synthetic UV filters could have adverse effects not only on humans but also on the environment. Even though lignin, the second most abundant renewable resource on earth, is a natural UV-absorbing polymer, its unfavorable dark color hampers its applications in sunscreens. In this work, we obtained light-colored lignin (CEL) from rice husks through cellulolytic enzyme treatment and subsequent solvent extraction under mild conditions and compared CEL to technical lignin from rice husks using the International Commission on Illumination L*a*b* (CIELAB) color space. Spherical nanoparticles of CEL (CEL-NP) were also prepared using a solvent shifting method and evaluated for broad-spectrum sunscreens. A moisturizing cream blended with CEL-NP exhibited higher sun protection factor (SPF) and UVA PF (protection factor) values than that with CEL. In addition, CEL-NP had synergistic effects when blended with an organic UV-filter sunscreen: CEL-NP enhanced the SPF and UVA PF values of the sunscreen greatly. However, there was no synergistic effect between CEL-NP and inorganic sunscreens. We expect nanoparticles of light-colored lignin to find high-value-added applications as a natural UV-blocking additive in sunscreens and cosmetics.
Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro
Authors: Shamsah Alyah H. , Cartmell Sarah H. , Richardson Stephen M. , Bosworth Lucy A.
Published: March 2020

The annulus fibrosus—one of the two tissues comprising the intervertebral disc—is susceptible to injury and disease, leading to chronic pain and rupture. A synthetic, biodegradable material could provide a suitable scaffold that alleviates this pain and supports repair through tissue regeneration. The transfer of properties, particularly biomechanical, from scaffold to new tissue is essential and should occur at the same rate to prevent graft failure post-implantation. This study outlines the effect of hydrolytic degradation on the material properties of a novel blend of polycaprolactone and poly(lactic acid) electrospun nanofibers (50:50) over a six-month period following storage in phosphate buffered saline solution at 37 °C. As expected, the molecular weight distribution for this blend decreased over the 180-day period. This was in line with significant changes to fiber morphology, which appeared swollen and merged following observation using Scanning Electron Microscopy. Similarly, hydrolysis resulted in considerable remodeling of the scaffolds’ polymer chains as demonstrated by sharp increases in percentage crystallinity and tensile properties becoming stiffer, stronger and more brittle over time. These mechanical data remained within the range reported for human annulus fibrosus tissue and their long-term efficacy further supports this novel blend as a potential scaffold to support tissue regeneration.
Influence of Heat Sink on the Mold Temperature of Gypsum Mold Used in Injection Molding
Authors: Lin Chung-Chih , Chen Kun-Chen , Yeh Hon-Chih
Published: March 2020

Gypsum molds have been developed as an alternative for the Rapid tooling (RT) method used in injection molding. However, the poor capability of the heat delivery forces the gypsum mold to operate under a high-risk condition, and distortion of the molded part becomes apparent. The goal is to investigate the effect of a heat sink on the reduction of the gypsum mold temperature and to establish a methodology for the heat sink design. The methodology used the advantage of the electrical circuit concept to analyze the mold temperature. The heat transfer of a mold was modeled using an equivalent thermal circuit. After all the components on the circuit were determined, the heat transfer rate could then be calculated. Once the heat transfer rate was known, the mold temperature could be easily analyzed. A modified thermal circuit considering transverse heat conduction was also proposed, which estimated the mold temperature more accurately. The mold temperature was reduced by 16.8 °C when a gypsum mold was installed with a 40 mm thick heat sink in a parallel configuration. Moreover, the reduction of the mold temperature improved the deflection of the molded part from 0.78 mm to 0.54 mm. This work provides a quick approach to analyze the mold temperature based on the thermal circuit concept. As the cooling system of the mold was modularized analytically, important properties of the cooling system in the heat transfer process were revealed by analyzing the thermal circuit of the mold, for example, the heat transfer rate or the mold temperature.
Bio-Nanocomposite Hydrogel Based on Zinc Alginate/Graphene Oxide: Morphology, Structural Conformation, Thermal Behavior/Degradation, and Dielectric Properties
Authors: Sabater i Serra Roser , Molina-Mateo José , Torregrosa-Cabanilles Constantino , Andrio-Balado Andreu , Meseguer Dueñas José María , Serrano-Aroca Ángel
Published: March 2020

Bio-nanocomposite hydrogels based on sodium alginate (SA) as polymer matrix and graphene oxide (GO) nanosheets with zinc as crosslinking agent were synthesized with the aim of incorporating the intrinsic properties of their constituents (bioactivity and antimicrobial activity). Thus, stable and highly interconnected networks were obtained from GO nanosheets dispersed in SA matrices through interactions with low amounts of zinc. The GO nanosheets were successfully incorporated into the alginate matrix in the form of a complex nano-network involving different interactions: Bonds between alginate chains induced by Zn ions (egg box structure), interactions between GO nanosheets through Zn ions and hydrogen bonds between alginate chains, and GO nanosheets. The molecular interactions and morphology were confirmed by Fourier-transform infrared spectroscopy and transmission electron microscopy. The composite’s structural organization showed enhanced thermal stability. The glass transition temperature shifted to a higher temperature due to the reduced mobility induced by additional crosslinking bonds after incorporating the GO nanosheets and Zn into the polymer matrix. Finally, the dielectric behavior revealed that charge carrier mobility was hampered by the compact structure of the nanonetwork, which reduced conductivity. The combined properties of these nanocomposite hydrogels make them attractive biomaterials in the field of regenerative medicine and wound care since both surface bioactivity and antibacterial behavior are two critical factors involved in the success of a biomaterial.
The Effect of Dielectric Polarization Rate Difference of Filler and Matrix on the Electrorheological Responses of Poly(ionic liquid)/Polyaniline Composite Particles
Authors: Zheng Chen , Lei Qi , Zhao Jia , Zhao Xiaopeng , Yin Jianbo
Published: March 2020

By using different conductivity of polyaniline as filler, a kind of poly(ionic liquid)/polyaniline composite particles was synthesized to investigate the influence of dielectric polarization rate difference between filler and matrix on the electrorheological response and flow stability of composite-based electrorheological fluids under simultaneous effect of shear and electric fields. The composite particles were prepared by a post ion-exchange procedure and then treated by ammonia or hydrazine to obtain different conductivity of polyaniline. Their electrorheological response was measured by dispersing these composite particles in insulating carrier liquid under electric fields. It showed that the composite particles treated by ammonia had the strongest electrorheological response and most stable flow behavior in a broad shear rate region from 0.5 s−1 to 1000 s−1. By using dielectric spectroscopy, it found that the enhanced electrorheological response with stable flow depended on the matching degree of the dielectric polarization rates between poly(ionic liquid) matrix and polyaniline filler. The closer their polarization rates are, the more stable the flow curves are. These results are helpful to design optimal composite-based electrorheological materials with enhanced and stable ER performance.
Catalytic Fast Pyrolysis of Poly (Ethylene Terephthalate) (PET) with Zeolite and Nickel Chloride
Authors: Jia Hang , Ben Haoxi , Luo Ying , Wang Rui
Published: March 2020

The pyrolysis of poly (ethylene terephthalate) (PET) in the presence of ZSM-5 zeolite and NiCl2 as a catalyst was studied at different temperatures under N2 atmosphere. Quantitative 13C nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FT-IR) were applied to characterize the waxy and solid residue. The carboxyl and aliphatic hydroxyl groups in the waxy residue have been greatly depleted after the use of zeolite during pyrolysis on the basis of the results of 13C NMR and FT-IR analysis. The proportion of aromatic hydroxyl groups increased by 21.82% when the mass ratio of zeolite to PET was set to 2.0/1.0. The results indicate that ZSM-5 is able to facilitate the decomposition of carboxyl, aliphatic groups, and ether bonds in the primary products produced from the pyrolysis of PET. In addition, the deoxygenation effects on the waxy products have been significantly enhanced with the addition of zeolite based on the results of NMR.
Investigation on the Continuous Wave Mode and the ms Pulse Mode Fiber Laser Drilling Mechanisms of the Carbon Fiber Reinforced Composite
Authors: Li Xiao , Hou Wentao , Han Bing , Xu Lingfei , Li Zewen , Nan Pengyu , Ni Xiaowu
Published: March 2020

The near infrared (NIR) laser drilling of a carbon fiber reinforced polymer (CFRP) composite in the continuous wave (CW) mode and the ms pulse mode was investigated by an experiment and a numerical simulation. The relationships between the laser penetrating time, entrance hole diameter, surface heat affected zone (HAZ) width, and material ablation rate and the laser irradiation time and laser peak power densities were obtained from the experiment. For the same average power density of the laser output, 3.5 kW/cm2, it was found that the ms pulse laser mode, which had a higher peak power density, had a higher drilling efficiency. When drilling the same holes, the pulse laser mode, which had the highest peak power density of 49.8 kW/cm2, had the lowest drilling time of 0.23 s and had the smallest surface HAZ width of 0.54 mm. In addition, it was found that the laser penetrating time decreased sharply when the peak power density was higher than 23.4 kW/cm2. After analyzing the internal gas pressure by the numerical simulation, it was considered that a large internal gas pressure appeared, which resulted from polymer pyrolysis, causing a large amount of the mechanical erosion of the composite material to improve the drilling efficiency. Therefore, the ms pulse laser showed its potential and advantage in laser drilling the CFRP composite.
Reliability Analysis of FRP-Confined Concrete at Ultimate using Conjugate Search Direction Method
Authors: Keshtegar Behrooz , Gholampour Aliakbar , Ozbakkaloglu Togay , Zhu Shun-Peng , Trung Nguyen-Thoi
Published: March 2020

In this paper compressive strength and ultimate strain results in the current database of fiber-reinforced polymer (FRP)-confined concrete are used to determine the reliability of their design space. The Lognormal, Normal, Frechet, Gumbel, and Weibull distributions are selected to evaluate the probabilistic characteristics of six FRP material categories. Following this, safety levels of the database are determined based on a probabilistic model. An iterative reliability method is developed with conjugate search direction for evaluating the reliability. The results show that Lognormal and Gumbel distributions provide best probability distribution for model errors of strength and strain enhancement ratios. The developed conjugate reliability method provides improved robustness over the existing reliability methods owing to its faster convergence to stable results. The results reveal that the part of the database containing normal strength concrete (NSC) heavily confined (i.e., actual confinement ratio (flu,a/f’co) > 0.5) by low and normal modulus carbon fibers (i.e., fiber elastic modulus (Ef) ≤ 260 GPa) and moderately confined (i.e., 0.3 ≤ flu,a/f’co ≤ 0.5) by aramid fibers exhibits a very high safety level. The segments of the database with a low and moderate safety level have been identified as i) NSC moderately and heavily confined by higher modulus glass fibers (i.e., Ef > 60 GPa), ii) high strength concrete (HSC) moderately and heavily confined (i.e., flu,a/f’co > 0.3) by glass fibers, iii) HSC lightly confined (i.e., flu,a/f’co ≤ 0.2) by carbon fibers, and iv) HSC lightly confined by aramid fibers. Additional experimental studies are required on these segments of the database before they can be used reliably for design and modeling purposes.
Material Properties Influencing the Charge Decay of Electret Filters and their Impact on Filtration Performance
Authors: Lee Jinwook , Kim Jooyoun
Published: March 2020

Electret filters as opposed to mechanical filters display the enhanced ability to capture airborne particles with the electrostatic attraction. However, the environmental aging during shelf-life or use may cancel its benefit by dissipating the charges. This work investigates the polymeric attributes influencing the charge decay and the electrostatic filtration of electret filters, employing polymers with different dielectric constants (εr) and wettability. As accelerated aging, high temperature (120 °C) or high humidity (25 °C, 90% RH) was applied to the electret filters for 48 h. For the humidity aging, wetting property of material was a critical factor affecting the charge decay and the filtration performance, as the absorbed water increases the electrical conductivity. For the thermal aging, the material with the highest εr deteriorated the electric potential and the filtration performance by the largest extent, due to the lower band gap energy for charge transfer. The results of this study implicate that εr and wettability are important material parameters influencing the electric conductivity and chain mobility, and they can be used as convenient predictors for charge retention capacity affecting the robust electrostatic filtration performance.
Theoretical Insights into the Structures and Capacitive Performances of Confined Ionic Liquids
Authors: Yang Jie , Ding Yajun , Lian Cheng , Ying Sanjiu , Liu Honglai
Published: March 2020

Room-temperature ionic liquids (RTILs) together with nano-porous electrodes are the most promising materials for supercapacitors and batteries. Many theoretical works have addressed the structures and performances of RTILs inside nanopores. However, only limited attention has been given to how the dispersion forces of RTILs influence the behavior of ions inside the slit pores. Toward this aim, we investigate the effects of various dispersion forces between ions on the macroscopic structures in nanoconfinement and the capacitance performance of supercapacitors by the classical density functional theory (CDFT). The results show that the dispersion force can significantly change the mechanism of the charging process and even the shape of differential capacitance curves. In addition, the voltage-dependent structures of RTILs with appropriate dispersion force appears in a given silt pore, which leads to extremely high capacitance and enhances the energy storage density. We hope that this work could further offer guidance for the optimizing of electrolytes for electrical double layer capacitors, like tuning the dispersion force between ions by adding/removing certain chemical groups on the cations and anions of RTILs.
Synthesis, Physical, Mechanical and Antibacterial Properties of Nanocomposites Based on Poly(vinyl alcohol)/Graphene Oxide–Silver Nanoparticles
Authors: Cobos Mónica , De-La-Pinta Iker , Quindós Guillermo , Fernández María , Fernández María
Published: March 2020

The design of new materials with antimicrobial properties has emerged in response to the need for preventing and controlling the growth of pathogenic microorganisms without the use of antibiotics. In this study, partially reduced graphene oxide decorated with silver nanoparticles (GO–AgNPs) was incorporated as a reinforcing filler with antibacterial properties to poly(vinyl alcohol) (PVA) for preparation of poly(vinyl alcohol)/graphene oxide-silver nanoparticles nanocomposites (PVA/GO–AgNPs). AgNPs, spherical in shape and with an average size of 3.1 nm, were uniformly anchored on the partially reduced GO surface. PVA/GO–AgNPs nanocomposites showed exfoliated structures with improved thermal stability, tensile properties and water resistance compared to neat PVA. The glass transition and crystallization temperatures of the polymer matrix increased with the incorporation of the hybrid. The nanocomposites displayed antibacterial activity against Staphylococcus aureus and Escherichia coli in a filler content- and time-dependent manner. S. aureus showed higher susceptibility to PVA/GO–AgNPs films than E. coli. Inhibitory activity was higher when bacterial cells were in contact with nanocomposite films than when in contact with leachates coming out of the films. GO–AgNPs based PVA nanocomposites could find application as wound dressings for wound healing and infection prevention.
Evaluation of Polymeric Materials for Chemical Enhanced Oil Recovery
Authors: Scott Alison J. , Romero-Zerón Laura , Penlidis Alexander
Published: March 2020

Polymer flooding is a promising enhanced oil recovery (EOR) technique; sweeping a reservoir with a dilute polymer solution can significantly improve the overall oil recovery. In this overview, polymeric materials for enhanced oil recovery are described in general terms, with specific emphasis on desirable characteristics for the application. Application-specific properties should be considered when selecting or developing polymers for enhanced oil recovery and should be carefully evaluated. Characterization techniques should be informed by current best practices; several are described herein. Evaluation of fundamental polymer properties (including polymer composition, microstructure, and molecular weight averages); resistance to shear/thermal/chemical degradation; and salinity/hardness compatibility are discussed. Finally, evaluation techniques to establish the polymer flooding performance of candidate EOR materials are described.
Steady-State Water Drainage by Oxygen in Anodic Porous Transport Layer of Electrolyzers: A 2D Pore Network Study
Authors: Altaf Haashir , Vorhauer Nicole , Tsotsas Evangelos , Vidaković-Koch Tanja
Published: March 2020

Recently, pore network modelling has been attracting attention in the investigation of electrolysis. This study focuses on a 2D pore network model with the purpose to study the drainage of water by oxygen in anodic porous transport layers (PTL). The oxygen gas produced at the anode catalyst layer by the oxidation of water flows counter currently to the educt through the PTL. When it invades the water-filled pores of the PTL, the liquid is drained from the porous medium. For the pore network model presented here, we assume that this process occurs in distinct steps and applies classical rules of invasion percolation with quasi-static drainage. As the invasion occurs in the capillary-dominated regime, it is dictated by the pore structure and the pore size distribution. Viscous and liquid film flows are neglected and gravity forces are disregarded. The curvature of the two-phase interface within the pores, which essentially dictates the invasion process, is computed from the Young Laplace equation. We show and discuss results from Monte Carlo pore network simulations and compare them qualitatively to microfluidic experiments from literature. The invasion patterns of different types of PTLs, i.e., felt, foam, sintered, are compared with pore network simulations. In addition to this, we study the impact of pore size distribution on the phase patterns of oxygen and water inside the pore network. Based on these results, it can be recommended that pore network modeling is a valuable tool to study the correlation between kinetic losses of water electrolysis processes and current density.
Finite Element Analysis in Setting of Fillings of V-Shaped Tooth Defects Made with Glass-Ionomer Cement and Flowable Composite
Authors: Dikova Tsanka , Vasilev Tihomir , Hristova Vesela , Panov Vladimir
Published: March 2020

The aim of the present paper is to investigate the deformation–stress state of fillings of V-shaped tooth defects by finite element analysis (FEA). Two different materials are used—auto-cured resin-reinforced glass-ionomer cement (GIC) and flowable photo-cured composite (FPC). Two materials are placed into the cavity in one portion, as before the application of the composite the cavity walls are covered with a thin adhesive layer. Deformations and equivalent von Mises stresses are evaluated by FEA. Experimental study of micro-leakage is performed. It is established that there is an analogous non-homogeneous distribution of equivalent Von Mises stresses at fillings of V-shaped defects, made with GIC and FPC. Maximum stresses are generated along the boundaries of the filling on the vestibular surface of the tooth and at the bottom of the filling itself. Values of equivalent Von Mises stresses of GIC fillings are higher than that of FPC. Magnitude and character of deformation distribution at GIC and FPC fillings are similar—deformation is maximum along the vestibular surface of the filling and is 0.056 and 0.053 mm, respectively. In FPC fillings, the adhesive layer, located along the cavity/filling boundary, is characterized with greatest strain. The experimental study of micro-leakage has confirmed the adequacy of models used in FEA.
Optimum Design of a Standalone Solar Photovoltaic System Based on Novel Integration of Iterative-PESA-II and AHP-VIKOR Methods
Authors: Ridha Hussein Mohammed , Gomes Chandima , Hizam Hashim , Ahmadipour Masoud , Muhsen Dhiaa Halboot , Ethaib Saleem
Published: March 2020

Solar energy is considered one of the most important renewable energy resources, and can be used to power a stand-alone photovoltaic (SAPV) system for supplying electricity in a remote area. However, inconstancy and unpredictable amounts of solar radiation are considered major obstacles in designing SAPV systems. Therefore, an accurate sizing method is necessary to apply in order to find an optimal configuration and fulfil the required load demand. In this study, a novel hybrid sizing approach was developed on the basis of techno-economic objectives to optimally size the SAPV system. The proposed hybrid method consisted of an intuitive method to estimate initial numbers of PV modules and storage battery, an iterative approach to accurately generate a set of wide ranges of optimal configurations, and a Pareto envelope-based selection algorithm (PESA-II) to reduce large configuration by efficacy obtaining a set of Pareto front (PF) solutions. Subsequently, the optimal configurations were ranked by using an integrated analytic hierarchy process (AHP) and vlsekeriterijumskaoptimizacija i kompromisonoresenje (VIKOR). The techno-economic objectives were loss of load probability, life cycle cost, and levelized cost of energy. The performance analysis results demonstrated that the lead–acid battery was reliable and more cost-effective than the other types of storage battery.
A Molecular Force Field-Based Optimal Deployment Algorithm for UAV Swarm Coverage Maximization in Mobile Wireless Sensor Network
Authors: Wang Xi , Tan Guan-zheng , Lu Fan-Lei , Zhao Jian , Dai Yu-si
Published: March 2020

In the mobile wireless sensor network (MWSN) field, there exists an important problem—how can we quickly form an MWSN to cover a designated working area on the ground using an unmanned aerial vehicle (UAV) swarm? This problem is of significance in many military and civilian applications. In this paper, inspired by intermolecular forces, a novel molecular force field-based optimal deployment algorithm for a UAV swarm is proposed to solve this problem. A multi-rotor UAV swarm is used to carry sensors and quickly build an MWSN in a designated working area. The necessary minimum number of UAVs is determined according to the principle that the coverage area of any three UAVs has the smallest overlap. Based on the geometric properties of a convex polygon, two initialization methods are proposed to make the initial deployment more uniform, following which, the positions of all UAVs are subsequently optimized by the proposed molecular force field-based deployment algorithm. Simulation experiment results show that the proposed algorithm, when compared with three existing algorithms, can obtain the maximum coverage ratio for the designated working area thanks to the proposed initialization methods. The probability of falling into a local optimum and the computational complexity are reduced, while the convergence rate is improved.
Distributed Secondary Control for Islanded Microgrids Cluster Based on Hybrid-Triggered Mechanisms
Authors: Weng Shengxuan , Xue Yusheng , Luo Jianbo , Li Yanman
Published: March 2020

Considering the communication resources limitation, the hybrid-triggered mechanism based distributed control of islanded microgrids cluster is proposed, which can restore the frequency to the rated value and realize the active power sharing when the disturbance occurs. The hybrid-triggered mechanism consists of the self- and event-triggered mechanisms, which are configured at each leader and follower distributed generation to determine the inter-microgrids and intra-microgrid information transmission, respectively. The communication burdens can be sharply reduced since the information is transmitted aperiodically only when the proposed triggering conditions are satisfied under the hybrid-triggered mechanism. Moreover, Zeno behavior is analyzed to be avoided to make the hybrid-triggered mechanism reasonable and practicable for practical islanded microgrids cluster. The simulation verifies the effectiveness of theoretical results.
Preparation of Nano-Porous Carbon-Silica Composites and Its Adsorption Capacity to Volatile Organic Compounds
Authors: Fu Lipei , Zhu Jiahui , Huang Weiqiu , Fang Jie , Sun Xianhang , Wang Xinya , Liao Kaili
Published: March 2020

Carbon-silica composites with nanoporous structures were synthesized for the adsorption of volatile organic compounds (VOCs), taking tetraethyl orthosilicate (TEOS) as the silicon source and activated carbon powder as the carbon source. The preparation conditions were as follows: the pH of the reaction system was 5.5, the hydrophobic modification time was 50 h, and the dosage of activated carbon was 2 wt%. Infrared spectrum analysis showed that the activated carbon was dispersed in the pores of aerogel to form the carbon-silica composites material. The static adsorption experiments, dynamic adsorption-desorption experiments, and regeneration experiments show that the prepared carbon-silica composites have microporous and mesoporous structures, the adsorption capacity for n-hexane is better than that of conventional hydrophobic silica gel, and the desorption performance is better than that of activated carbon. It still has a high retention rate of adsorption capacity after multiple adsorption-desorption cycles. The prepared carbon-silica composites material has good industrial application prospects in oil vapor recovery, providing a new alternative for solving organic waste gas pollution.
Eucalyptus Kraft Lignin as an Additive Strongly Enhances the Mechanical Resistance of Tree-Leaf Pellets
Authors: Clavijo Leonardo , Zlatanovic Slobodan , Braun Gerd , Bongards Michael , Dieste Andrés , Barbe Stéphan
Published: March 2020

Pelleted biomass has a low, uniform moisture content and can be handled and stored cheaply and safely. Pellets can be made of industrial waste, food waste, agricultural residues, energy crops, and virgin lumber. Despite their many desirable attributes, they cannot compete with fossil fuel sources because the process of densifying the biomass and the price of the raw materials make pellet production costly. Leaves collected from street sweeping are generally discarded in landfills, but they can potentially be valorized as a biofuel if they are pelleted. However, the lignin content in leaves is not high enough to ensure the physical stability of the pellets, so they break easily during storage and transportation. In this study, the use of eucalyptus kraft lignin as an additive in tree-leaf pellet production was studied. Results showed that when 2% lignin is added the abrasion resistance can be increased to an acceptable value. Pellets with added lignin fulfilled all requirements of European standards for certification except for ash content. However, as the raw material has no cost, this method can add value or contribute to financing continued sweeping and is an example of a circular economy scenario.
Religious Struggle and Psychological Well-Being: The Mediating Role of Religious Support and Meaning Making
Authors: Zarzycka Beata , Tychmanowicz Anna , Krok Dariusz
Published: March 2020

Although a variety of studies have found robust links between religious/spiritual (r/s) struggle and poorer well-being, only a few have examined the means by which r/s struggle affects mental well-being. The present paper aims to examine religious support and meaning making as mediators of the relationship between r/s struggle and well-being. The study included 226 adults, 108 women and 118 men, aged between 17 and 78 years. We applied the Religious and Spiritual Struggle Scale, Religious Support Scale, Meaning Making Scale, and Psychological Well-Being Scale. The results demonstrated that both religious support and meaning making were mediators in the relationship between r/s struggles and well-being. During moral or demonic struggles, many people reportedly feel supported by their religion, make meaning based on these positive religious experiences, and in turn experience greater well-being. Conversely, during divine, ultimate meaning, and interpersonal struggles people may feel like God does not support them, which may lead to difficulties reframing their religious experience, and adversely influence well-being. The findings from this study underscore the multifaceted character of r/s struggle: during different types of r/s struggle people may feel that religion is a source of support for them or, by contrast, they may feel deprived of religious support, which can lead to an increase or decrease of well-being, respectively.