bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the Seismic Behavior of Rammed Earth Walls with an L-Form Cross-Section
Authors: Bui Quoc-Bao , Bui Tan-Trung , Tran Mai-Phuong , Bui Thi-Loan , Le Hoang-An
Published: March 2019

Rammed earth (RE) is a construction material which is made by compacting the soil in a formwork. This material is attracting the attention of the scientific community due to its sustainable characteristics. Among different aspects to be investigated, the seismic performance remains an important topic which needs advanced investigations. The existing studies in the literature have mainly adopted simplified approaches to investigate the seismic performance of RE structures. The present paper adopts a numerical approach to investigate the seismic behavior of RE walls with an L-form cross-section. The 3D FEM model used can take into account the plasticity and damage of RE layers and the interfaces. The model was first validated by an experimental test presented in the literature. Then, the model was employed to assess the seismic performance of a L-form wall of a RE house at different amplitudes of earthquake excitations. Influences of the cross-section form on the earthquake performance of RE walls were also investigated. The results show that the L-form cross-section wall has a better seismic performance than a simple rectangular cross-section wall with similar dimensions. For the L-form cross-section wall, the damage observed concentrates essentially on the connection between two flanges of the wall.
Game Modelling and Strategy Research on the System Dynamics–Based Quadruplicate Evolution for High–Speed Railway Operational Safety Supervision System
Authors: Li Kehong , Wang Wenke , Zhang Yadong , Zheng Tao , Guo Jin
Published: March 2019

In view of the entrusted transportation management model (ETMM) of China’s high–speed railway (HSR), the supervision strategy of an HSR company for its multiple agents plays a very important role in ensuring the safety and sustainable development of HSR. Due to the existence of multiple agents in ETMM, the supervision strategy for these agents is usually difficult to formulate. In this study, a quadruplicate HSR safety supervision system evolutionary game model composed of an HSR company and three agents was established through the analysis of the complex game relationship existing in the system. The behavioral characteristics and the steady state of decision–making of all stakeholders involved in the system are proved by evolutionary game theory and system dynamics simulation. The results show that there will be long–term fluctuations in the strategies selected by the four stakeholders in the static reward–penalty control scenario (RPCS), which indicates that an evolutionary stable strategy does not exist. With increases in the reward–penalty coefficient, the fluctuations are intensified. Therefore, the dynamic RPCS was proposed to control the fluctuations, and the simulation was repeated. The results show that the fluctuations can be effectively restrained by adopting the dynamic RPCS, but if the coefficients are the same, the static RPCS is better than the dynamic RPCS for increasing the safety investment rate of the three agents. This demonstrates that the HSR company should apply these two control scenarios flexibly according to the actual situation when formulating a supervision strategy in order to effectively control and enhance the safety level of HSR operations when multiple agents are involved.
Identifying the Critical Risks in Railway Projects Based on Fuzzy and Sensitivity Analysis: A Case Study of Belt and Road Projects
Authors: Andrić Jelena M. , Wang Jiayuan , Zhong Ruoyu
Published: March 2019

The Belt and Road Initiative (BRI) is a Chinese development strategy developed in order to establish connectivity and deepen cooperation between China and other countries, increase trade, and support the socio-economic development of vast regions. A significant number of railway projects are planned to be constructed under BRI since railways are the most efficient means of transportation. This paper investigates the critical risks in railway projects implemented under BRI. In total, 24 potential risks in BRI railway projects are identified and categorized into 6 groups. A questionnaire survey is conducted in order to collect data about the probability of risk occurrence and their impact. To identify the critical risks in railway projects, a novel method based on fuzzy and sensitivity analysis is developed and applied for risk assessment. This method uses a fuzzy synthetic evaluation approach to assess risks and sensitivity analysis as criteria for critical risk identification. The results show that the most critical risks in railway projects are changes in design, design errors, cooperation between China and BRI country, loan risk, complex geological conditions of terrain, and geopolitical risk. The theoretical contribution of this paper is a novel method which combines fuzzy and sensitivity analysis into a single approach.
Socioeconomic Development, Demographic Dynamics and Forest Fires in Italy, 1961–2017: A Time-Series Analysis
Authors: Carlucci Margherita , Zambon Ilaria , Colantoni Andrea , Salvati Luca
Published: March 2019

Empirical studies investigating long-term trends in wildfires’ frequency and severity have been relatively scarce in Europe. Number of fire events, total burnt area and average fire size were studied between 1961 and 2017 in Italy with the aim to identify homogeneous time periods with similar wildfire frequency and severity and correlate them with the background socioeconomic context. Fire attributes had a diverging behavior over time: the number of fires was the highest in the 1970s and the early 1980s; total burnt area was relatively more constant over time with a peak in the 1980s; and, finally, average fire size decreased quite homogeneously from the peak observed in the 1960s and early 1970s. The number of fires and average fire size were significantly influenced by the value of the same variable one year before. Investigating long-term historical outlines of forest fires, a mixed approach based on time-series statistical analysis, multivariate techniques and regressive models intended to define changes in fire regimes and socioeconomic development. In fact, the comparative valuation of the socioeconomic aspects and wildfire trends can reveal a key step to recognizing mitigation and preventive possibilities. Through a multivariate analysis, a substantial difference in the socioeconomic profile can emerge by decade, evidencing a (more or less) rapid socioeconomic development in relation to the evolution of forest fires in Italy.
The Effects of Latent Attitudinal Variables and Sociodemographic Differences on Travel Behavior in Two Small, Underdeveloped Cities in China
Authors: Cheng Gang , Zhao Shuzhi , Li Li
Published: March 2019

In small, underdeveloped Chinese cities, residents have few options for transportation and travel service problems have not received enough attention from the authorities. This study examines residents’ preferred mode of travel in Lhasa and Yushu, China, two small and underdeveloped cities in which travelers tend to be economically disadvantaged. Travel data from different regions was analyzed to explore their commonalities. A structural equation model with latent variables is proposed to capture the heterogeneity not observed in the selection process. Results indicate that four of the six latent variables—preference for comfort, preference for reliability, preference for convenience and safety consciousness—are more helpful than preferences for flexibility and environmental awareness in explaining transportation utility, which could reflect residents’ travel behavior. Based on the results, respondents were divided into five groups with similar travel preferences through the k-means clustering method. The findings show that ensuring high comfort and convenience and moderate safety and reliability is conducive to increasing residents’ use of public transport. Furthermore, an examination of residents’ sociodemographic differences reflects that, in the future, active transport demand management should focus on trying to satisfy the preferences of female, low-income and elderly travelers.
On the Relationship between Innovation Activity and Manufacturing Upgrading of Emerging Countries: Evidence from China
Authors: Xie Zhong , Zang Guixue , Wu Feifei
Published: March 2019

Most research into the relationship between innovation activity and manufacturing upgrading has been conducted in developed economies, such as the countries of North America and Europe. Due to the non-replicability of the developed countries’ development modes, most emerging countries cannot directly copy the manufacturing upgrading path of the developed countries. However, knowledge about the relationship between innovation activity and manufacturing upgrading in emerging economies remains limited. This paper sheds light on the relationship between innovation activity and manufacturing upgrading in emerging countries from the following three types of innovation, namely, technical innovation, product innovation, and institutional innovation. By using data from Chinese provinces for the period 2001–2015, this paper empirically investigates the relationship between innovation activity and manufacturing upgrading in emerging countries. The results show that technical innovation, product innovation, and institutional innovation have significantly positive driving-force effects on manufacturing upgrading, which indicates that innovation is an important source of promoting manufacturing upgrading for emerging countries. Moreover, the effect above is more prominent for technical innovation. The results are resilient to the alternative indicators of innovation and the alternative indicators of manufacturing upgrading. This paper provides a theoretical and empirical reference for conducting innovation-incentive policy and promoting the optimization of manufacturing structure.
Do Consumers Want to Pay for Green Electricity? A Case Study from Poland
Authors: Kowalska-Pyzalska Anna
Published: March 2019

This paper analyses the willingness to pay (WTP) for green electricity among residential consumers in Poland, which is the sixth most populous and eighth largest and most dynamic economy in the European Union. The current share of renewable energy sources (RES) in energy consumption is around 13.5% and is expected to increase. To estimate the mean WTP of the residential consumers in Poland, as well as its main determinants, an empirical survey has been conducted and a contingent valuation method has been used. The results obtained indicate that—above all—age, income, environmental attitudes, peer support, but also education and knowledge play the most important role in explaining consumers’ WTP for green electricity. Statistical analyses indicate that the mean WTP of Polish households is currently quite low (additional 3.5 USD per month), which is due to the relatively low GDP per capita, lack of knowledge about green energy and no past experience with green electricity tariffs.
The Concentrations and Removal Effects of PM10 and PM2.5 on a Wetland in Beijing
Authors: Li Chunyi , Huang Yilan , Guo Huanhuan , Wu Gaojie , Wang Yifei , Li Wei , Cui Lijuan
Published: March 2019

Particulate matter (PM) is an essential source of atmospheric pollution in metropolitan areas since it has adverse effects on human health. However, previous research suggested wetlands can remove particulate matter from the atmosphere to land surfaces. This study was conducted in the Hanshiqiao Wetland National Nature Reserve in Beijing during 2016. The concentrations of PM10 and PM2.5 on a wetland and bare land in the park, as well as metrological data, were collected during the whole year. Based on the observed data, removal efficiency of each land use type was calculated by empirical models and the relationships between concentrations and metrological factors were also analyzed. The results indicated that: (1) In general, the PM10 and PM2.5 concentrations on the bare land surface were higher than those on the wetland surface, in both of which the highest value appeared at night and evening, while the lowest value appeared near noon. In terms of season, the average concentration of PM10 was higher in winter (wetland: 137.48 μg·m−3; bare land: 164.75 μg·m−3) and spring (wetland: 205.18 μg·m−3; bare land: 244.85 μg·m−3) in general. The concentration of PM2.5 on the wetland surface showed the same pattern, while that on the bare land surface was higher in spring and summer. (2) Concentrations of PM10 and PM2.5 were significantly correlated with the relative humidity (p < 0.01) and inversely correlated with wind speed (p < 0.05). The relationship between PM10 and PM2.5 concentrations and temperature was more complicated—it showed a significantly negative correlation (p < 0.01) between them in winter and spring, however, the correlation was insignificant in autumn. In summer, only the correlation between PM10 concentration and temperature on the wetland surface was significant (p < 0.01). (3) The dry removal efficiency of PM10 was greater than that of PM2.5. The dry removal efficiencies of PM10 and PM2.5 followed the order of spring > winter > autumn > summer on the wetland. This study seeks to provide practical measures to improve air quality and facilitate sustainable development in Beijing.
An Empirical Study on the Acceptance of 4D BIM in EPC Projects in China
Authors: Gong Pan , Zeng Ningshuang , Ye Kunhui , König Markus
Published: March 2019

The engineering-procurement-construction (EPC) method has the potential to help construction projects achieve sustainable performance, e.g., the contractor’s early involvement, cost savings, and a reduced schedule. However, high uncertainties and complexities are contained in EPC projects. 4D BIM (Building Information Modeling) with abilities to simplify the time and space relationships of construction activities and support multi-party information sharing is beneficial to EPC project management. The behavior pattern of the project personnel toward accepting 4D BIM information systems or tools needs to be explored. Therefore, a research model of the acceptance of 4D BIM in EPC projects with eight latent constructs is proposed through a literature review of technology acceptance theories. Data is collected from a questionnaire survey and interviews. Research hypotheses are examined using PLS-SEM (partial least squares-structural equation modeling). Empirical evidence is collected from China, and implications to the developing countries facing the challenge of developing a technology-intensive construction industry are provided: (1) Adopting 4D BIM in the EPC project is beneficial; (2) the task-technology fit plays a leading role in technology acceptance; (3) the management incentive is inefficient at the operational stage. Suggestions for future research on 4D BIM acceptance in complex construction projects with abundant data and alternative models are provided.
Impact of Political Connections on Corporate Environmental Performance: From a Green Development Perspective
Authors: Zhang Liming , Ye Fei , Yang Li , Zhou Guichuan
Published: March 2019

Employing a sample consisting of Chinese A-share listed companies, this study carries out an empirical research to investigate the influence path of political connections on enterprise environmental performance. The results show a strong positive association between political connections and corporate environmental performance, and green technology innovation plays the mediating role between them. In addition, public participation negatively moderates the relationship between political connections and corporate environmental performance. When the level of public participation is higher, the relationship between political connections and corporate environmental performance becomes weaker.
Exploring Residents’ Perceptions of Mega Event-Dubai Expo 2020: A Pre-Event Perspective
Authors: Vij Mohit , Upadhya Amitabh , Vij Anu , Kumar Manoj
Published: March 2019

This study explores the residents’ perceptions of economic, socio-cultural and environmental impacts of a mega-event (World Expo 2020) to be held in Dubai, as well as their level of inclination to participate in the event. The event organizers announced a requirement of thirty thousand volunteers to assist them in successfully organizing the mega event. Such events could prove to be landmarks in destination brand building and may lead to an increase in the number of tourists. The purpose of the study is to gauge the level of understanding about the event impacts within the resident population and their willingness to get involved in event organization. Priory studies of perceptions about the economic, cultural and environmental impacts of a mega-event, are well-nigh non-existent, especially for World Expositions. This study explores residents’ perception to get a fair idea of how they get involved in a mega event that will surely have profound impacts on the destination. Drawing on stakeholder theory and triple bottom line (TBL) model, the study uses primary data collected from more than two hundred fifty residents of the region, through a structured questionnaire. The data analysis follows a descriptive design supported by SEM and path analysis. Major findings indicate favorable and promising responses from the residents, and brings forth the scope to improve the level of involvement of the residents to make the event a success.
A Markov Regime Switching Approach towards Assessing Resilience of Romanian Collective Investment Undertakings
Authors: Badea Leonardo , Armeanu Daniel Ştefan , Panait Iulian , Gherghina Ştefan Cristian
Published: March 2019

This paper explores the sensitivity of Romanian collective investment undertakings’ returns to changes in equity, fixed income and foreign exchange market returns. We use a sample of 80 open-end investment funds and pension funds with daily returns between 2016 and 2018. Our methodology consists of measuring changes in the daily conditional volatility for the fund returns (EGARCH) and changes in their conditional correlation with selected market risk factors (DCC MV-GARCH) throughout different volatility regimes identified using a Markov Regime Switching model. We argue that, on average, the level of conditional correlations between funds and market risk factors remained stable and unconcerned by the volatility regimes. In addition, for only less than half of the funds in the sample, their volatility regimes were synchronized with those of the selected market risk factors. We found that, on average, fund returns are more correlated with equity returns and less correlated with changes in local bond yields, while not being significantly influenced by changes in foreign bond yields or changes in foreign exchange. During the period investigated equity returns were the most volatile while the funds returns volatility were, on average, much more reduced. Overall, our results show the resilience of the Romanian collective investment sector to the selected market risk factors, during the investigated period.
Analysis of Factors Affecting Hit-and-Run and Non-Hit-and-Run in Vehicle-Bicycle Crashes: A Non-Parametric Approach Incorporating Data Imbalance Treatment
Authors: Zhou Bei , Li Zongzhi , Zhang Shengrui , Zhang Xinfen , Liu Xin , Ma Qiannan
Published: March 2019

Hit-and-run (HR) crashes refer to crashes involving drivers of the offending vehicle fleeing incident scenes without aiding the possible victims or informing authorities for emergency medical services. This paper aims at identifying significant predictors of HR and non-hit-and-run (NHR) in vehicle-bicycle crashes based on the classification and regression tree (CART) method. An oversampling technique is applied to deal with the data imbalance problem, where the number of minority instances (HR crash) is much lower than that of the majority instances (NHR crash). The police-reported data within City of Chicago from September 2017 to August 2018 is collected. The G-mean (geometric mean) is used to evaluate the classification performance. Results indicate that, compared with original CART model, the G-mean of CART model incorporating data imbalance treatment is increased from 23% to 61% by 171%. The decision tree reveals that the following five variables play the most important roles in classifying HR and NHR in vehicle-bicycle crashes: Driver age, bicyclist safety equipment, driver action, trafficway type, and gender of drivers. Several countermeasures are recommended accordingly. The current study demonstrates that, by incorporating data imbalance treatment, the CART method could provide much more robust classification results.
Repulsive Effect of Stroboscopic Light Barriers on Native Salmonid (Salmo trutta) and Cyprinid (Pseudochondrostoma duriense and Luciobarbus bocagei) Species of Iberia
Authors: Jesus Joaquim , Teixeira Amílcar , Natário Silvestre , Cortes Rui
Published: March 2019

A repulsive effect, that some induced primary stimuli, like sound and light, is known to be provoked in fish behavior. In the present study, two strobe light frequencies, 350 flashes/minute and 600 flashes/minute, were tested in laboratorial conditions, using three native freshwater fish species of northern Portugal: Brown trout (Salmo trutta), Northern straight-mouth nase (Pseudochondrostoma duriense) and Iberian barbel (Luciobarbus bocagei). The results showed a differential repulsive behavior of the fish species to light stimulus, and particularly to a frequency of 600 flashes/minute. S. trutta presented the most repulsive behavior, whereas the L. bocagei showed less repulsion to the light stimulus. No relevant differences were found between pre-test and post-assessments, confirming a rapid recovery of natural fish behavior after the deterrent effect. The results highlighted the potential of behavioral barriers, particularly in salmonid streams, based on strobe light stimulus.
Sustainable Development of Urban Rail Transit Networks: A Vulnerability Perspective
Authors: Shi Jiangang , Wen Shiping , Zhao Xianbo , Wu Guangdong
Published: March 2019

Urban rail transit (URT) systems are critical to modern public transportation services. Unfortunately, disruptions in URT systems can lead to dysfunction and threaten sustainable development. This study analyses URT network sustainability from a vulnerability perspective. Two network attack scenarios, including random attacks and intentional attacks, are designed to assess different kinds of disruptions to URT networks. Under random attacks, nodes are randomly removed from the network. In contrast, under intentional attacks, key nodes are identified and removed based on topological metrics and passenger flow volume. Then, URT network vulnerability is evaluated by quantifying the changes in network efficiency and structural integrity under the network attacks from a spatio-temporal point of view. The real-world case of the Shanghai URT system from 1993 to 2020 is used to illustrate the vulnerability in the evolution of the URT system. The results indicate that the URT network is increasingly fault-tolerant and structurally robust over time. The URT network is more vulnerable to intentional attacks than to random failures. Additionally, there are significant spatial differences in the vulnerability of Shanghai URT network. Stations in the central activity zone (CAZ) are more fault-tolerant and robust than stations located outside of the CAZ. Furthermore, stations with large centrality and greater passenger flow volumes and lines with many key nodes and greater passenger flow volumes, are vulnerable to disruptions in the URT networks. This study provides a new index to comprehensively quantify node centrality; it also fills a research gap by analysing the vulnerability of URT networks based on both longitudinal and spatial patterns. Finally, this paper highlights significant practical implications for the sustainable development of URT networks, as well as the sustainable development of public transportation services.
Hysteretic Loops in Correlation with the Maximum Dissipated Energy, for Linear Dynamic Systems
Authors: Bratu Polidor
Published: March 2019

This paper presents the outcomes of the theoretical and experimental research carried out on a real model at natural scale using Voigt–Kelvin linear viscoelastic type m, c, and k models excited by a harmonic force F(t) = F0 sinωt, where F0 is the amplitude of the harmonic force and ω is the excitation angular frequency. The linear viscous-elastic rheological system (m, c, k) is characterized by the fact that the c linear viscous damping—and, consequently, the fraction of the critical damping ζ—may be changed so that the dissipated energy can reach maximum W d max values. The optimization condition between the W d max maximum dissipated energy and the amortization ζ 0 = ± ( 1 − Ω 2 ) / 2 Ω modifies the structure of the relation F = F(x), which describes the elliptical hysteresis loop F–x in the sense that it has its large axis making an angle less than 90° with respect to the x-axis in Ω < 1 ante-resonance, and an angle greater than 90° in post-resonance for Ω > 1 . The elliptical Q–x hysteretic loops are tilted with their large axis only at angles below 90°. It can be noticed that the equality between the arias of the hysteretic loop, in the two representations systems Q–x and F–x, is verified, both being equal with the maximum dissipated energy W d max .
Residual Stress Analysis of an Orthotropic Composite Cylinder under Thermal Loading and Unloading
Authors: Zarandi Somayeh Bagherinejad , Lai Hsiang-Wei , Wang Yun-Che , Aizikovich Sergey
Published: March 2019

Elastoplastic analysis of a composite cylinder, consisting of an isotropic elastic inclusion surrounded by orthotropic matrix, is conducted via numerical parametric studies for examining its residual stress under thermal cycles. The matrix is assumed to be elastically and plastically orthotropic, and all of its material properties are temperature-dependent (TD). The Hill’s anisotropic plasticity material model is adopted. The interface between the inclusion and matrix is perfectly bonded, and the outer boundary of the cylinder is fully constrained. A quasi-static, uniform temperature field is applied to the cylinder, which is analyzed under the plane-strain assumption. The mechanical responses of the composite cylinder are strongly affected by the material symmetry and temperature-dependent material properties. When the temperature-independent material properties are assumed, larger internal stresses at the loading phase are predicted. Furthermore, considering only yield stress being temperature dependent may be insufficient since other TD material parameters may also affect the stress distributions. In addition, plastic orthotropy inducing preferential yielding along certain directions leads to complex residual stress distributions when material properties are temperature-dependent.
Interaction between Floral Merism and Symmetry: Evidence from Fasciated Mutant of Lupinus angustifolius L. (Leguminosae)
Authors: Sinjushin Andrey A. , Bykova Ekaterina A. , Choob Vladimir V.
Published: March 2019

A significant diversity of flower structure in angiosperms is defined by few parameters, among which one may list floral merism and symmetry as the most important. However, observation of normal variation in different taxa indicates that high floral merism (more than six organs) is very rarely associated with a monosymmetry. Precise mechanisms underlying this tendency, as well as the mode of interaction between regulatory pathways of floral symmetry and merism, remain unidentified. In this work, we observed the floral ontogeny in normal plants of Lupinus angustifolius L. (Leguminosae), described the floral structure and its variations in fasciated mutant. Our results suggest that monosymmetry is regulated independently from the floral merism, so three types of petals differentiate properly even in flowers with an anomalously high number of organs. However, the adaxial floral domain is the most stable floral part, which is the least susceptible to the fluctuations of merism. This means that some genes responsible for the development of the adaxial flower domain may additionally stabilize it and hence prevent fluctuations of merism in the adaxial domain. The monosymmetry in Leguminosae is a character connected with pollination by insects and requires a precise interaction between all floral parts. That is why fluctuations, which impair this interaction and symmetry, may decrease the pollination efficiency.
Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
Authors: Tuchscherr Lorena , Pöllath Christine , Siegmund Anke , Deinhardt-Emmer Stefanie , Hoerr Verena , Svensson Carl-Magnus , Thilo Figge Marc , Monecke Stefan , Löffler Bettina
Published: March 2019

Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types of tissue infections. For this, we collected 10–12 clinical S. aureus strains each from nasal colonization, and from patients with endoprosthesis infection, hematogenous osteomyelitis, and sepsis. All strains were characterized by genotypic analysis, and by the expression of virulence factors. The host–pathogen interaction was studied through several functional assays in osteoblast cultures. Additionally, selected strains were tested in a murine sepsis/osteomyelitis model. We did not find characteristic bacterial features for the defined infection types; rather, a wide range in all strain collections regarding cytotoxicity and invasiveness was observed. Interestingly, all strains were able to persist and to form small colony variants (SCVs). However, the low-cytotoxicity strains survived in higher numbers, and were less efficiently cleared by the host than the highly cytotoxic strains. In summary, our results indicate that not only destructive, but also low-cytotoxicity strains are able to induce infections. The low-cytotoxicity strains can successfully survive, and are less efficiently cleared from the host than the highly cytotoxic strains, which represent a source for chronic infections. The understanding of this interplay/evolution between the host and the pathogen during infection, with specific attention towards low-cytotoxicity isolates, will help to optimize treatment strategies for invasive and therapy-refractory infection courses.
Structurally Robust and Functionally Highly Versatile—C-Type Lectin (-Related) Proteins in Snake Venoms
Authors: Eble Johannes A.
Published: March 2019

Snake venoms contain an astounding variety of different proteins. Among them are numerous C-type lectin family members, which are grouped into classical Ca2+- and sugar-binding lectins and the non-sugar-binding snake venom C-type lectin-related proteins (SV-CLRPs), also called snaclecs. Both groups share the robust C-type lectin domain (CTLD) fold but differ in a long loop, which either contributes to a sugar-binding site or is expanded into a loop-swapping heterodimerization domain between two CLRP subunits. Most C-type lectin (-related) proteins assemble in ordered supramolecular complexes with a high versatility of subunit numbers and geometric arrays. Similarly versatile is their ability to inhibit or block their target molecules as well as to agonistically stimulate or antagonistically blunt a cellular reaction triggered by their target receptor. By utilizing distinct interaction sites differentially, SV-CLRPs target a plethora of molecules, such as distinct coagulation factors and receptors of platelets and endothelial cells that are involved in hemostasis, thrombus formation, inflammation and hematogenous metastasis. Because of their robust structure and their high affinity towards their clinically relevant targets, SV-CLRPs are and will potentially be valuable prototypes to develop new diagnostic and therapeutic tools in medicine, provided that the molecular mechanisms underlying their versatility are disclosed.
Development of an Antifungal and Antimycotoxigenic Device Containing Allyl Isothiocyanate for Silo Fumigation
Authors: Quiles Juan Manuel , Nazareth Tiago de Melo , Luz Carlos , Luciano Fernando Bittencourt , Mañes Jordi , Meca Giuseppe
Published: March 2019

The aims of this study were to evaluate the antifungal activity of the bioactive compound allyl isothiocyanate (AITC) against Aspergillus flavus (8111 ISPA) aflatoxins (AFs) producer and Penicillium verrucosum (D-01847 VTT) ochratoxin A (OTA) producer on corn, barley, and wheat. The experiments were carried out initially in a simulated silo system for laboratory scale composed of glass jars (1 L). Barley and wheat were contaminated with P. verrucosum and corn with A. flavus. The cereals were treated with a hydroxyethylcellulose gel disk to which 500 µL/L of AITC were added; the silo system was closed and incubated for 30 days at 21 °C. After that, simulated silos of 100 L capacity were used. Barley, wheat, and corn were contaminated under the same conditions as the previous trial and treated with disks with 5 mL of AITC, closed and incubated for 90 days at 21 °C. In both cases, the control test did not receive any antifungal treatment. The growth of the inoculated fungi and the reduction in the formation of AFs and OTA were determined. In the lab scale silo system, complete inhibition of fungal growth at 30 days has been observed. In corn, the reduction of aflatoxin B1 (AFB1) was 98.5%. In the 100 L plastic drums, a significant reduction in the growth of A. flavus was observed, as well as the OTA formation in wheat (99.5%) and barley (92.0%).
Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis
Authors: Wang Rui , Dong Zhaoyue , Zhang Xiaolong , Mao Jingxin , Meng Fancheng , Lan Xiaozhong , Liao Zhihua , Chen Min
Published: March 2019

Pterocephalus hookeri (C. B. Clarke) Höeck, recorded in the Chinese Pharmacopoeia (2015 version) as a Tibetan medicine for the treatment of various diseases, especially rheumatoid arthritis, was believed to possess a slight toxicity. However, hardly any research has been carried out about it. The present study aimed to evaluate the toxicity in vivo and in vitro. Toxicity was observed by the evaluation of mice weight loss and histopathological changes in the liver. Then, the comparison research between ethyl acetate extract (EAE) and n-butanol extract (BUE) suggested that liver toxicity was mainly induced by BUE. The mechanical study suggested that BUE-induced liver toxicity was closely associated with necrosis detected by MTT and propidium iodide (PI) staining, via releasing lactate dehydrogenase (LDH), reducing the fluidity, and increasing the permeability of the cell membrane. Western blot analysis confirmed that the necrosis occurred molecularly by the up-regulation of receptor-interacting protein kinase 1 (RIP1) and receptor-interacting protein kinase 3 (RIP3), as well as the activation of the nuclear factor-kappa-gene binding (NF-κB) signaling pathway in vivo and in vitro. This finding indicated that the liver toxicity induced by BUE from P. hookeri was mainly caused by necrosis, which provides an important theoretical support for further evaluation of the safety of this folk medicine.
Efficacy of Early Endoscopic Intervention for Restoring Normal Swallowing Function in Patients with Lateral Medullary Infarction
Authors: Kang Sun Hyung , Kim Ju Seok , Joo Jong Seok , Eun Hyuk Soo , Lee Eaum Seok , Moon Hee Seok , Kim Seok Hyun , Sung Jae Kyu , Lee Byung Seok , Jeong Hyun Yong , Kim Yeongwook , Sohn Min Kyun , Jee Sungju
Published: March 2019

Dysphagia is considered to be a significant barrier for recovery after lateral medullary infarction (LMI). However, there is still no gold standard treatment for dysphagia. The aim of this study was to explore an effect of an early treatment options for swallowing dysfunction after acute LMI. Medical records of acute LMI patients who had been admitted to the department of rehabilitation medicine from January 2014 to December 2017 were reviewed retrospectively. We compared the clinical efficacy of conventional dysphagia rehabilitation to early endoscopic intervention using either botulinum toxin injection into cricopharyngeal muscle or endoscopic balloon dilatation of the muscle. Outcomes, such as duration of parental feeding, albumin level at diet transition to enteral feeding, and complications, were analyzed. A total of 18 patients with LMI were included. While eight patients (8/9, 88.89%) in the endoscopic group were capable of orally ingesting their diet after intervention, the conversion from tube feeding to an oral diet was possible in only five patients (5/9, 55.56%) of the conventional group during hospitalization. However, the difference between the two groups was not significant (p-value ≤ 0.147, chi-square test). Only the final dietary level at the time of discharge was higher level in endoscopic group. The conversion interval from tube feeding to oral diet was also comparable between groups. There was no re-conversion from the oral diet to tube feeding in patients of either group during the median follow-up period of 20 months. Early endoscopic intervention may be a better option for dysphagia with LMI, compared to conventional dysphagia rehabilitation. However, a larger and prospective trial may be needed to confirm our observations.
Granulovirus GP37 Facilitated ODVs Cross Insect Peritrophic Membranes and Fuse with Epithelia
Authors: Liu Xiangyang , Fang Wei , Fan Rui , Zhang Linna , Lei Chengfeng , Zhang Jingjing , Nian Wenkai , Dou Tao , An Shiheng , Zhou Lin , Sun Xiulian
Published: March 2019

The Cydia pomonella granulovirus (CpGV) GP37 has synergistic effects on the infectivity of nucleopolyhedroviruses (NPVs), however, the mechanism employed is unclear. In this study, in vitro and in vivo binding assays indicated that GP37 efficiently bound to the midgut peritrophic membrane (PM) of Spodoptera exigua larvae. Treatment with GP37 led to the damage of the PM’s compacted structure and the generation of the PM perforations, and the enhancement of the PM’s permeability. qPCR results further demonstrated that GP37 increased the ability of occlusion-derived virions (ODV) to cross the PM. R18-labeling experiments exhibited that GP37 also promoted the fusion of ODVs and insect midgut epithelia. Altogether, our present results revealed that the synergistic mechanism of GP37 to the infectivity of NPV might involve two parts. GP37 damaged the integrity of the PM after binding, which enhanced the PM’s permeability and increased the ability of ODVs to cross the PM, finally facilitating the ODVs reaching the midgut. In addition, GP37 promoted the fusion of ODVs and insect midgut epithelia. Our data expand the understanding of the mechanism used by baculovirus synergistic factors and provide a foundation for the development of high-efficiency baculoviral insecticides.
Discovery and Characterization of Bukakata orbivirus (Reoviridae:Orbivirus), a Novel Virus from a Ugandan Bat
Authors: Fagre Anna , Lee Justin , Kityo Robert , Bergren Nicholas , Mossel Eric , Nakayiki Teddy , Nalikka Betty , Nyakarahuka Luke , Gilbert Amy , Peterhans Julian , Crabtree Mary , Towner Jonathan , Amman Brian , Sealy Tara , Schuh Amy , Nichol Stuart , Lutwama Julius , Miller Barry , Kading Rebekah
Published: March 2019

While serological and virological evidence documents the exposure of bats to medically-important arboviruses, their role as reservoirs or amplifying hosts is less well-characterized. We describe a novel orbivirus (Reoviridae:Orbivirus) isolated from an Egyptian fruit bat (Rousettus aegyptiacus leachii) trapped in 2013 in Uganda and named Bukakata orbivirus. This is the fifth orbivirus isolated from a bat, however genetic information had previously only been available for one bat-associated orbivirus. We performed whole-genome sequencing on Bukakata orbivirus and three other bat-associated orbiviruses (Fomede, Ife, and Japanaut) to assess their phylogenetic relationship within the genus Orbivirus and develop hypotheses regarding potential arthropod vectors. Replication kinetics were assessed for Bukakata orbivirus in three different vertebrate cell lines. Lastly, qRT-PCR and nested PCR were used to determine the prevalence of Bukakata orbivirus RNA in archived samples from three populations of Egyptian fruit bats and one population of cave-associated soft ticks in Uganda. Complete coding sequences were obtained for all ten segments of Fomede, Ife, and Japanaut orbiviruses and for nine of the ten segments for Bukakata orbivirus. Phylogenetic analysis placed Bukakata and Fomede in the tick-borne orbivirus clade and Ife and Japanaut within the Culicoides/phlebotomine sandfly orbivirus clade. Further, Bukakata and Fomede appear to be serotypes of the Chobar Gorge virus species. Bukakata orbivirus replicated to high titers (106–107 PFU/mL) in Vero, BHK-21 [C-13], and R06E (Egyptian fruit bat) cells. Preliminary screening of archived bat and tick samples do not support Bukakata orbivirus presence in these collections, however additional testing is warranted given the phylogenetic associations observed. This study provided complete coding sequence for several bat-associated orbiviruses and in vitro characterization of a bat-associated orbivirus. Our results indicate that bats may play an important role in the epidemiology of viruses in the genus Orbivirus and further investigation is warranted into vector-host associations and ongoing surveillance efforts.
Can Bats Serve as Reservoirs for Arboviruses?
Authors: Fagre Anna C. , Kading Rebekah C.
Published: March 2019

Bats are known to harbor and transmit many emerging and re-emerging viruses, many of which are extremely pathogenic in humans but do not cause overt pathology in their bat reservoir hosts: henipaviruses (Nipah and Hendra), filoviruses (Ebola and Marburg), and coronaviruses (SARS-CoV and MERS-CoV). Direct transmission cycles are often implicated in these outbreaks, with virus shed in bat feces, urine, and saliva. An additional mode of virus transmission between bats and humans requiring further exploration is the spread of disease via arthropod vectors. Despite the shared ecological niches that bats fill with many hematophagous arthropods (e.g., mosquitoes, ticks, biting midges, etc.) known to play a role in the transmission of medically important arboviruses, knowledge surrounding the potential for bats to act as reservoirs for arboviruses is limited. To this end, a comprehensive literature review was undertaken examining the current understanding and potential for bats to act as reservoirs for viruses transmitted by blood-feeding arthropods. Serosurveillance and viral isolation from either free-ranging or captive bats are described in relation to four arboviral groups (Bunyavirales, Flaviviridae, Reoviridae, Togaviridae). Further, ecological associations between bats and hematophagous viral vectors are characterized (e.g., bat bloodmeals in mosquitoes, ingestion of mosquitoes by bats, etc). Lastly, knowledge gaps related to hematophagous ectoparasites (bat bugs and bed bugs (Cimicidae) and bat flies (Nycteribiidae and Streblidae)), in addition to future directions for characterization of bat-vector-virus relationships are described.
Measurement of Permeability and Comparison of Pavements
Authors: Chen Lu-Ming , Chen Jui-Wen , Chen Ting-Hao , Lecher Timothy , Davidson Paul C.
Published: March 2019

Permeable pavements have the ability to reduce surface runoff by allowing water to infiltrate into the underlying soil. The potential of permeable pavements to assist in managing stormwater and improve water quality has gained attention as an option, other than conventional impermeable concrete for paving purposes. This study examined the permeability of three different pavement systems, including the JW Eco-technology pavement (JW), which has not previously been installed or studied in the U.S., standard impermeable concrete (IC), and pervious concrete (PC). Each pavement type was installed in triplicate. Devices based on the ASTM C1701/C1701M and ASTM C1781/C1781M constant-head methods, the National Center for Asphalt Technology (NCAT) falling-head permeameter, and two new square frames, SF-4 and SF-9, modified to fit the JW pavement, were utilized for permeability measurement on several locations of each pavement system. The results showed that the JW Eco-technology pavement had comparable permeability to the commonly used PC pavement in each method used. In addition, there was a strong correlation between the permeability measurements of NCAT method and SF-4, and between the ASTM standard and SF-9. The square frames used in this study showed their effectiveness and efficiency in performing permeability measurements. It was also found that the permeability obtained had a pronounced difference in values between the falling head and the constant head methods, with an average ratio ranging from 4.08–6.36.
Strategy for Flux Enhancement in Biofilm Ceramic Membrane Bioreactor Applying Prepolymerized and Non-Prepolymerized Inorganic Coagulants
Authors: Kulesha Olga , Maletskyi Zakhar , Kvaal Knut , Ratnaweera Harsha
Published: March 2019

Considering new legislative and economic restrictions caused by the water crisis, this work focuses on a more efficient wastewater treatment process, which combines biological treatment in a moving bed biofilm system with a membrane bioreactor (BF-MBR) and coagulation, particularly addressing fouling alleviation in the separation stage. The study justifies the positive impact of coagulant dosing in BF-MBR regarding membrane flux and fouling rate. Statistical techniques connect the results of coagulation and membrane separation experiments with properties of mixed liquor, obtained after biotreatment in the representative pilot plant and characteristics of prepolymerized and non-prepolymerized inorganic coagulants. Research results substantiate the need for a pH-controlled coagulation of mixed liquor in BF-MBR depending on coagulant type, which influences charge, hydrophobicity and size of flocs and organic content of the system. It is suggested, that the adsorption/charge neutralization mechanism dominates in flux enhancement in BF-MBR, giving the best results in the case of prepolymerized aluminium coagulants. Together with high quality of permeate, the application of prepolymerized aluminium chloride of medium basicity entails a tenfold increase in filtration time of the membrane separation cycle and increases net membrane flux by 30–56%. The results of the study are practically significant for the development of an automated control system for BF-MBR, optimizing treatment rates together with membrane separation efficiency.
Evaluation of Four GLUE Likelihood Measures and Behavior of Large Parameter Samples in ISPSO-GLUE for TOPMODEL
Authors: Cho Huidae , Park Jeongha , Kim Dongkyun
Published: March 2019

We tested four likelihood measures including two limits of acceptability and two absolute model residual methods within the generalized likelihood uncertainty estimation (GLUE) framework using the topography model (TOPMODEL). All these methods take the worst performance of all time steps as the likelihood of a model and none of these methods were successful in finding any behavioral models. We believe that reporting this failure is important because it shifted our attention from which likelihood measure to choose to why these four methods failed and how to improve these methods. We also observed how large parameter samples impact the performance of a hybrid uncertainty estimation method, isolated-speciation-based particle swarm optimization (ISPSO)-GLUE using the Nash–Sutcliffe (NS) coefficient. Unlike GLUE with random sampling, ISPSO-GLUE provides traditional calibrated parameters as well as uncertainty analysis, so over-conditioning the model parameters on the calibration data can affect its uncertainty analysis results. ISPSO-GLUE showed similar performance to GLUE with a lot less model runs, but its uncertainty bounds enclosed less observed flows. However, both methods failed in validation. These findings suggest that ISPSO-GLUE can be affected by over-calibration after a long evolution of samples and imply that there is a need for a likelihood measure that can better explain uncertainties from different sources without making statistical assumptions.