bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of prophylactic methylprednisolone on reducing the risk of post-extubation stridor in patients after an emergency intubation: study protocol for a randomized controlled trial
Authors: Wang Jingyi , Walline Joseph Harold , Yin Lu , Dai Yili , Dai Jiayuan , Zhu Huadong , Yu Xuezhong , Xu Jun
Published: January 2021

Background

Post-extubation stridor (PES) is one of the most common complications of invasive respiratory support, with severe cases leading to possible extubation failure (reintubation within 48 h) and increased mortality. Previous studies confirmed that prophylactic corticosteroids play an important role in reducing the risk of PES and extubation failure. However, few studies have looked at the efficacy of corticosteroids on preventing PES in patients after an emergency intubation.

Aim

To evaluate whether a single dose of methylprednisolone given over a set timeframe before extubation is effective in preventing PES in patients after an emergency intubation.

Methods

A multicenter, randomized, placebo-controlled trial will be performed in an emergency department (ED) setting. The trial will include 132 patients who fail a cuff-leak test (CLT) prior to the intervention. Patients will be randomly assigned to either intravenous methylprednisolone (40 mg) or placebo 4 h prior to extubation. Other eligible patients who pass the CLT will be included in a non-intervention (observation) group. The primary endpoint is the incidence of PES within 48 h after extubation. Secondary endpoints include oxygen therapy, respiratory support requirements, reintubation secondary to PES, adverse effects within 48 h after extubation, hospital length of stay, and hospital mortality.

Discussion

Patients who are intubated on an emergency basis have a higher risk of intubation-related complications. Previous studies have examined treatment regimens involving more than 10 different variations on corticosteroid treatments for PES prevention, while for ED therapy, only a simple and effective treatment would be appropriate. Corticosteroid administration is usually accompanied by adverse effects; thus, this study will be important for further risk stratification among intubated ED patients.

Trial registration

Chictr.org.cn ChiCTR2000030349. Registered on 29 February 2020.
Modulating redox metabolism to improve isobutanol production in Shimwellia blattae
Authors: Acedos Miguel G. , de la Torre Isabel , Santos Victoria E. , García-Ochoa Félix , García José L. , Galán Beatriz
Published: January 2021

Background

Isobutanol is a candidate to replace gasoline from fossil resources. This higher alcohol can be produced from sugars using genetically modified microorganisms. Shimwellia blattae (p424IbPSO) is a robust strain resistant to high concentration of isobutanol that can achieve a high production rate of this alcohol. Nevertheless, this strain, like most strains developed for isobutanol production, has some limitations in its metabolic pathway. Isobutanol production under anaerobic conditions leads to a depletion of NADPH, which is necessary for two enzymes in the metabolic pathway. In this work, two independent approaches have been studied to mitigate the co-substrates imbalance: (i) using a NADH-dependent alcohol dehydrogenase to reduce the NADPH dependence of the pathway and (ii) using a transhydrogenase to increase NADPH level.

Results

The addition of the NADH-dependent alcohol dehydrogenase from Lactococcus lactis (AdhA) to S. blattae (p424IbPSO) resulted in a 19.3% higher isobutanol production. The recombinant strain S. blattae (p424IbPSO, pIZpntAB) harboring the PntAB transhydrogenase produced 39.0% more isobutanol than the original strain, reaching 5.98 g L−1 of isobutanol. In both strains, we observed a significant decrease in the yields of by-products such as lactic acid or ethanol.

Conclusions

The isobutanol biosynthesis pathway in S. blattae (p424IbPSO) uses the endogenous NADPH-dependent alcohol dehydrogenase YqhD to complete the pathway. The addition of NADH-dependent AdhA leads to a reduction in the consumption of NADPH that is a bottleneck of the pathway. The higher consumption of NADH by AdhA reduces the availability of NADH required for the transformation of pyruvate into lactic acid and ethanol. On the other hand, the expression of PntAB from E. coli increases the availability of NADPH for IlvC and YqhD and at the same time reduces the availability of NADH and thus, the production of lactic acid and ethanol. In this work it is shown how the expression of AdhA and PntAB enzymes in Shimwellia blattae increases yield from 11.9% to 14.4% and 16.4%, respectively.
Biomass, lipid accumulation kinetics, and the transcriptome of heterotrophic oleaginous microalga Tetradesmus bernardii under different carbon and nitrogen sources
Authors: Gao Baoyan , Wang Feifei , Huang Luodong , Liu Hui , Zhong Yuming , Zhang Chengwu
Published: January 2021

Background

Heterotrophic cultivation of microalgae has been proposed as a viable alternative method for novel high-value biomolecules, enriched biomass, and biofuel production because of their allowance of high cell density levels, as well as simple production technology. Tetradesmus bernardii, a newly isolated high-yielding oleaginous microalga under photoautotrophic conditions, is able to grow heterotrophically, meaning that it can consume organic carbon sources in dark condition. We investigated the effect of different carbon/nitrogen (C/N) ratios on the growth and lipid accumulation of T. bernardii in heterotrophic batch culture under two nitrogen sources (NaNO3 and CO(NH2)2). In addition, we conducted time-resolved transcriptome analysis to reveal the metabolic mechanism of T. bernardii in heterotrophic culture.

Results

T. bernardii can accumulate high biomass concentrations in heterotrophic batch culture where the highest biomass of 46.09 g/L was achieved at 100 g/L glucose concentration. The rate of glucose to biomass exceeded 55% when the glucose concentration was less than 80 g/L, and the C/N ratio was 44 at urea treatment. The culture was beneficial to lipid accumulation at a C/N ratio between 110 and 130. NaNO3 used as a nitrogen source enhanced the lipid content more than urea, and the highest lipid content was 45% of dry weight. We performed RNA-seq to analyze the time-resolved transcriptome of T. bernardii. As the nitrogen was consumed in the medium, nitrogen metabolism-related genes were significantly up-regulated to speed up the N metabolic cycle. As chloroplasts were destroyed in the dark, the metabolism of cells was transferred from chloroplasts to cytoplasm. However, storage of carbohydrate in chloroplast remained active, mainly the synthesis of starch, and the precursor of starch synthesis in heterotrophic culture may largely come from the absorption of organic carbon source (glucose). With regard to lipid metabolism, the related genes of fatty acid synthesis in low nitrogen concentration increased gradually with the extension of cultivation time.

Conclusion

T. bernardii exhibited rapid growth and high lipid accumulation in heterotrophic culture. It may be a potential candidate for biomass and biofuel production. Transcriptome analysis showed that multilevel regulation ensured the conversion from carbon to the synthesis of carbohydrate and lipid.
High concentrations of membrane-fed ivermectin are required for substantial lethal and sublethal impacts on Aedes aegypti
Authors: Hadlett Max , Nagi Sanjay C. , Sarkar Manas , Paine Mark J. I. , Weetman David
Published: January 2021

Background

With widespread insecticide resistance in mosquito vectors, there is a pressing need to evaluate alternatives with different modes of action. Blood containing the antihelminthic drug ivermectin has been shown to have lethal and sub-lethal effects on mosquitoes. Almost all work to date has been on Anopheles spp., but impacts on other anthropophagic vectors could provide new options for their control, or additional value to anti-malarial ivermectin programmes.

Methods

Using dose-response assays, we evaluated the effects of ivermectin delivered by membrane feeding on daily mortality (up to 14 days post-blood feed) and fecundity of an Indian strain of Aedes aegypti.

Results

The 7-day lethal concentration of ivermectin required to kill 50% of adult mosquitoes was calculated to be 178.6 ng/ml (95% confidence intervals 142.3–218.4) for Ae. aegypti, which is much higher than that recorded for Anopheles spp. in any previous study. In addition, significant effects on fecundity and egg hatch rates were only recorded at high ivermectin concentrations (≥ 250 ng/ul).

Conclusion

Our results suggest that levels of ivermectin present in human blood at current dosing regimes in mass drug administration campaigns, or even those in a recent higher-dose anti-malaria trial, are unlikely to have a substantial impact on Ae. aegypti. Moreover, owing to the strong anthropophagy of Ae. aegypti, delivery of higher levels of ivermectin in livestock blood is also unlikely to be an effective option for its control. However, other potential toxic impacts of ivermectin metabolites, accumulation in tissues, sublethal effects on behaviour, or antiviral action might increase the efficacy of ivermectin against Ae. aegypti and the arboviral diseases it transmits, and require further investigation.

Graphical Abstract
Canine infection with Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp., and Ehrlichia spp. in the United States, 2013–2019
Authors: Little Susan , Braff Jennifer , Place Joshua , Buch Jesse , Dewage Bhagya Galkissa , Knupp Andrew , Beall Melissa
Published: January 2021

Background

Dogs in the US are commonly infected with vector-borne pathogens, including heartworm and tick-borne disease agents. The geographic distribution of both arthropod vectors and the pathogens they transmit continues to expand.

Methods

To describe the current geographic distribution and prevalence of antigen of Dirofilaria immitis and antibody to Borrelia burgdorferi, Ehrlichia spp., and Anaplasma spp. in dogs, we summarized over 144 million test results from 2013 to 2019, inclusive, by county, state, and region. Canine seroprevalence by state was compared to population-adjusted human reports of tick-borne diseases.

Results

Results varied regionally, with D. immitis antigen and Ehrlichia spp. antibodies more frequently detected in the Southeast (2.6% and 5.2%, respectively) and antibody to B. burgdorferi and Anaplasma spp. most common in the Northeast (12.1% and 7.3%, respectively). Overall, percent positive test results to D. immitis decreased in the Southeast by 33.3% when compared to earlier summaries using the same strategy (from 3.9 to 2.6%). Geographic expansion of areas where dogs commonly test positive for Ehrlichia spp. was evident, likely because of a change in the test made in 2012 to allow detection of antibodies to E. ewingii concomitant with expansion of vector tick populations. Percent positive test results to Ehrlichia spp. increased in every region; this shift was particularly pronounced in the Southeast, where percent positive test results increased fourfold (from 1.3 to 5.2%). Continued geographic expansion of B. burgdorferi and A. phagocytophilum was apparent in the Northeast, Midwest, and Upper South, although canine seroprevalence of antibody to B. burgdorferi was much lower than prior surveys in many Lyme-endemic areas. Annual reports of human cases of Lyme disease, ehrlichiosis, and anaplasmosis were associated with percent positive canine results by state for the three tick-borne disease agents (R2 = 0.812, 0.521, and 0.546, respectively). Within endemic areas, percent positive test results for all three tick-borne agents demonstrated evidence of geographic expansion.

Conclusions

Large scale analysis of results from screening dogs in practice for evidence of vector-borne infections, including those with zoonotic importance, continues to be a valuable strategy for understanding geographic trends in infection risk over time.

Graphical Abstract
First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China
Authors: Deng Lei , Yao Jingxin , Chen Shanyu , He Tingmei , Chai Yijun , Zhou Ziyao , Shi Xiaogang , Liu Haifeng , Zhong Zhijun , Fu Hualin , Peng Guangneng
Published: January 2021

Background

Blastocystis sp. is an anaerobic protozoan that parasitizes many animal hosts and the human gastrointestinal tract, and its pathogenicity is controversial. Captive wildlife may be potential reservoirs for human infection with Blastocystis sp. The present study was performed to investigate the prevalence and subtype distribution of Blastocystis sp. in zoo animals in Sichuan Province, southwestern China.

Methods

A total of 420 fresh fecal samples were collected from 54 captive wildlife species in four zoos in southwestern China between June 2017 and September 2019. The prevalence and subtype (ST) genetic characteristics of Blastocystis sp. were determined by PCR amplification of the barcode region of the SSU rRNA gene and phylogenetic analysis.

Results

Overall, 15.7% (66/420) of the animal samples and 20.7% (14/54) of the species tested were shown to be infected with Blastocystis sp. The highest prevalence of Blastocystis sp. was found in Panzhihua Zoo (24.3%), which was significantly higher than that in Chengdu Zoo (6.9%), and Xichang Zoo (2.9%) (P < 0.05). There are also significant differences in the prevalence of Blastocystis sp. among different species (P < 0.05), and the highest of Blastocystis sp. prevalence was observed in white-cheeked gibbon, black great squirrel, and red giant flying squirrel (100%). Subtype analysis of Blastocystis sp. revealed nine subtypes, including six zoonotic STs (ST1-5, and ST8) and three animal-specific STs (ST10, ST14, and ST17), with ST17 as the predominant subtype (26/66) in Blastocystis sp.-positive isolates.

Conclusions

To our knowledge, this is the first report on the prevalence and subtype distribution of Blastocystis sp. among captive wildlife in zoos in southwestern China. This study highlights that these animals may serve as reservoirs for human Blastocystis sp. infections.

Graphical Abstract
Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids
Authors: Shalini Vasantha , Bhaduri Utsa , Ravikkumar Anjhana C. , Rengarajan Anusha , Satyanarayana Rao M. R.
Published: January 2021

Background

H1T2/H1FNT is a germ cell-specific linker histone variant expressed during spermiogenesis specifically in round and elongating spermatids. Infertile phenotype of homozygous H1T2 mutant male mice revealed the essential function of H1T2 for the DNA condensation and histone-to-protamine replacement in spermiogenesis. However, the mechanism by which H1T2 imparts the inherent polarity within spermatid nucleus including the additional protein partners and the genomic domains occupied by this linker histone are unknown.

Results

Sequence analysis revealed the presence of Walker motif, SR domains and putative coiled-coil domains in the C-terminal domain of rat H1T2 protein. Genome-wide occupancy analysis using highly specific antibody against the CTD of H1T2 demonstrated the binding of H1T2 to the LINE L1 repeat elements and to a significant percentage of the genic regions (promoter-TSS, exons and introns) of the rat spermatid genome. Immunoprecipitation followed by mass spectrometry analysis revealed the open chromatin architecture of H1T2 occupied chromatin encompassing the H4 acetylation and other histone PTMs characteristic of transcriptionally active chromatin. In addition, the present study has identified the interacting protein partners of H1T2-associated chromatin mainly as nucleo-skeleton components, RNA-binding proteins and chaperones.

Conclusions

Linker histone H1T2 possesses unique domain architecture which can account for the specific functions associated with chromatin remodeling events facilitating the initiation of histone to transition proteins/protamine transition in the polar apical spermatid genome. Our results directly establish the unique function of H1T2 in nuclear shaping associated with spermiogenesis by mediating the interaction between chromatin and nucleo-skeleton, positioning the epigenetically specialized chromatin domains involved in transcription coupled histone replacement initiation towards the apical pole of round/elongating spermatids.
A pilot randomized controlled trial of a tailored smoking cessation program for people living with HIV in the Washington, D.C. metropolitan area
Authors: Kierstead Elexis C. , Harvey Emily , Sanchez Denisse , Horn Kimberly , Abroms Lorien C. , Spielberg Freya , Stanton Cassandra A. , Debnam Charles , Cohn Amy M. , Gray Tiffany , Magnus Manya , Patel Minal , Niaura Raymond , Elf Jessica L.
Published: January 2021

Objective

Morbidity and mortality from smoking-related diseases among people living with HIV (PLWH) in the U.S. surpasses that due to HIV itself. Conventional smoking cessation treatments have not demonstrated strong efficacy among PLWH. We conducted a pilot randomized controlled trial (RCT) to evaluate a tailored smoking cessation intervention based on the minority stress model. We compared standard of care counseling (SOC) to a tailored intervention (TI) including one face-to-face counseling session incorporating cognitive behavioral therapy to build resilience, and 30 days of 2-way text messaging.

Results

The primary outcome was smoking cessation. Secondary outcomes included cigarettes per day (CPD), exhaled carbon monoxide (CO), and cessation self-efficacy. A total of 25 participants were enrolled (TI:11, SOC:14), and 2 were lost to follow-up. There were no significant differences in quit rates between study groups. However, there was a significantly greater decrease in CPD in the TI versus SOC (13.5 vs. 0.0, p-value:0.036). Additionally, self-efficacy increased in both groups (TI p-value:0.012, SOC p-value:0.049) and CO decreased in both groups (TI p-value: < 0.001, SOC p-value:0.049). This intervention shows promise to support smoking cessation among PLWH. A larger study is needed to fully evaluate the efficacy of this approach.

Clinical trial: Trial Registration: Retrospectively registered (10/20/2020) NCT04594109.
Code fusion information-hiding algorithm based on PE file function migration
Authors: Tian Zuwei , Yang Hengfu
Published: January 2021

PE (portable executable) file has the characteristics of diversity, uncertainty of file size, complexity of file structure, and singleness of file format, which make it easy to be a carrier of information hiding, especially for that of large hiding capacity. This paper proposes an information-hiding algorithm based on PE file function migration, which utilizes disassembly engine to disassemble code section of PE file, processes function recognition, and shifts the whole codes of system or user-defined functions to the last section of PE file. Then it hides information in the original code space. The hidden information is combined with the main functions of the PE file, and the hidden information is coupled with the key codes of the program, which further enhances the concealment performance and anti-attack capability of the system.
Quantum Hermite–Hadamard-type inequalities for functions with convex absolute values of second $q^{b}$-derivatives
Authors: Ali Muhammad Aamir , Budak Hüseyin , Abbas Mujahid , Chu Yu-Ming
Published: January 2021

In this paper, we obtain Hermite–Hadamard-type inequalities of convex functions by applying the notion of$q^{b}$qb-integral. We prove some new inequalities related with right-hand sides of$q^{b}$qb-Hermite–Hadamard inequalities for differentiable functions with convex absolute values of second derivatives. The results presented in this paper are a unification and generalization of the comparable results in the literature on Hermite–Hadamard inequalities.
Assessing the effect of urban form on social sustainability: a proposed ‘Integrated Measuring Tools Method’ for urban neighborhoods in Dubai
Authors: Alipour S. M. Hossein , Galal Ahmed Khaled
Published: January 2021

Among the three pillars of sustainability, social sustainability has received the least attention, especially among the studies addressing the sustainable housing development in Dubai’s urban neighborhoods. Unfortunately, these neighborhoods are not showing enough vibrancy of their public spaces and amenities which seemingly undermined their social sustainability. The lack of a comprehensive tool that can assess the interlinked indicators of social sustainability in urban neighborhoods has urged the research to propose an Integrated Measuring Tools Method (IMTM), that incorporates relevant computational simulation techniques alongside the appropriate conventional qualitative tools. The proposed IMTM was utilized in assessing the social sustainability indicators within the urban form of an existing neighborhood in Dubai. The assessment revealed that various urban form characteristics including; density, land-use, mobility options, mobility networks, streets layouts, and the built environment’s safety and aesthetic qualities, are the main factors undermining social sustainability in the studied locality. The results informed a set of suggested redevelopment strategies/guidelines for this neighborhood that takes into consideration the exiting constraints and opportunities. These included; increasing density by infill development, redefining land-use to achieve diversity in housing types and decentralized amenities, diversifying mobility options, improving the spatial integration of the mobility networks while restraining the private cars movement, improving accessibility, enriching urban landscape, and finally enhancing the quality of amenities and services. A social sustainability redevelopment scenario was suggested based on these strategies. When compared to the exiting neighborhood utilizing the IMTM, it proved significantly better attainment of social sustainability principles. So, it might be claimed that the proposed IMTM and its outcomes proved to be a valid basis for assessing, redeveloping and validating social sustainability of existing neighborhoods with similar typology in Dubai, and potentially in the region.
Analyzing Bangkok city taxi ride: reforming fares for profit sustainability using big data driven model
Authors: Phiboonbanakit Thananut , Horanont Teerayut
Published: January 2021

With the trend toward the use of large-scale vehicle probe data, an urban-scale analysis can now provide useful information for taxi drivers and passengers. Unfortunately, traffic congestion has become a critical problem in urban cities. Road traffic congestion reduces productivity in transportation services, and the daily profit earned is consequently reduced. This is opposite to the cost of living, which is increasing rapidly. Therefore, these issues are causing difficulties in all occupations in terms of managing daily expenses, particularly for taxi drivers. The taxi driving is classified as low income compared to other occupations. Such facts are a symbol of economic inefficiency. To this end, this study aims to assist taxi agencies and the government in improving taxi driver profits in Bangkok using large-scale data. To deal with these large-scale data, we propose a big data-driven model. With this model, we first calculate costs using a cost–distance algorithm and trip reconstruction. The data are then modeled to understand distance-based profits with respect to the departure time and traffic conditions. Finally, several cost predictive models using machine learning are evaluated using the ground truth from 50 taxis for a 1-month period. The experiment results show that more frequent trips over a short distance yield higher profits than long-distance trips. Finally, a solution to improve taxi driver profits is determined. We also compare the advantages and disadvantages of a unified solution.
Impact of climate change on flood inundation in a tropical river basin in Indonesia
Authors: Yamamoto Kodai , Sayama Takahiro , Apip
Published: January 2021

Climate change will have a significant impact on the water cycle and will lead to severe environmental problems and disasters in humid tropical river basins. Examples include river basins in Sumatra Island, Indonesia, where the coastal lowland areas are mostly composed of peatland that is a wetland environment initially sustained by flooding from rivers. Climate change may alter the frequency and magnitude of flood inundation in these lowland areas, disturbing the peatland environment and its carbon dynamics and damaging agricultural plantations. Consequently, projecting the extent of inundation due to future flooding events is considered important for river basin management. Using dynamically downscaled climate data obtained by the Non-Hydrostatic Regional Climate Model (NHRCM), the Rainfall-Runoff-Inundation (RRI) model was applied to the Batanghari River Basin (42,960 km2) in Sumatra Island, Indonesia, to project the extent of flood inundation in the latter part of the twenty-first century. In order to obtain reasonable estimates of the extent of future flood inundation, this study compared two bias correction methods: a Quantile Mapping (QM) method and a combination of QM and Variance Scaling (VS) methods. The results showed that the bias correction obtained by the QM method improved the simulated flow duration curve (FDC) obtained from the RRI model, which facilitated comparison with the simulated FDC using reference rainfall data. However, the high spatial variability observed in daily and 15-day rainfall data remained as the spatial variation bias, and this could not be resolved by simple QM bias correction alone. Consequently, the simulated extreme variables, such as annual maximum flood inundation volume, were overestimated compared to the reference data. By introducing QM-VS bias correction, the cumulative density functions of annual maximum discharge and inundation volumes were improved. The findings also showed that flooding will increase in this region; for example, the flood inundation volume corresponding to a 20-year return period will increase by 3.3 times. River basin management measures, such as land use regulations for plantations and wetland conservation, should therefore consider increases in flood depth and area, the extents of which under a future climate scenario are presented in this study.
Quantitative bone SPECT/CT reconstruction utilizing anatomical information
Authors: Kangasmaa Tuija S. , Constable Chris , Sohlberg Antti O.
Published: January 2021

Background

Bone SPECT/CT has been shown to offer superior sensitivity and specificity compared to conventional whole-body planar scanning. Furthermore, bone SPECT/CT allows quantitative imaging, which is challenging with planar methods. In order to gain better quantitative accuracy, Bayesian reconstruction algorithms, including both image derived and anatomically guided priors, have been utilized in reconstruction in PET/CT scanning, but they have not been widely used in SPECT/CT studies. Therefore, the aim of this work was to evaluate the performance of CT-guided reconstruction in quantitative bone SPECT.

Methods

Three Bayesian reconstruction methods were evaluated against the conventional ordered subsets expectation maximization (OSEM) reconstruction method. One of the studied Bayesian methods was the relative difference prior (RDP), which has recently gained popularity in PET reconstruction. The other two methods, anatomically guided smoothing prior (AMAP-S) and anatomically guided relative difference prior (AMAP-R), utilized anatomical information from the CT scan. The reconstruction methods were evaluated in terms of quantitative accuracy with artificial lesions inserted in clinical patient studies and with 20 real clinical patients. Maximum and mean standardized uptake values (SUVs) of the lesions were defined.

Results

The analyses showed that all studied Bayesian methods performed better than OSEM and the anatomical priors also outperformed RDP. The average relative error in mean SUV for the artificial lesion study for OSEM, RDP, AMAP-S, and AMAP-R was − 53%, − 35%, − 15%, and − 10%, when the CT study had matching lesions. In the patient study, the RDP method gave 16 ± 9% higher maximum SUV values than OSEM, while AMAP-S and AMAP-R offered increases of 36 ± 8% and 36 ± 9%, respectively. Mean SUV increased for RDP, AMAP-S, and AMAP-R by 18 ± 9%, 26 ± 5%, and 33 ± 5% when compared to OSEM.

Conclusions

The Bayesian methods with anatomical prior, especially the relative difference prior-based method (AMAP-R), outperformed OSEM and reconstruction without anatomical prior in terms of quantitative accuracy.
Pedagogical intentions or practical considerations when facilitating children's play? Teachers’ beliefs about the availability of play materials in the indoor ECEC environment
Authors: Nilsen Tone Rove
Published: January 2021

This qualitative study investigated teachers' beliefs about the availability of play materials in the physical indoor environment of early childhood education and care (ECEC). The empirical data were gathered from fieldwork in eight child groups in ECEC institutions across Norway and comprised 13 semistructured interviews with teachers. The findings indicate a common ideological understanding among teachers that play materials should be available in children's play, enhancing play, learning, and development possibilities. However, many teachers described putting play materials out of children's reach during times of play. There are variations, but practical considerations often override pedagogical intentions when teachers plan and facilitate children's play. This study aims to enhance the indoor ECEC environment's quality by stimulating teachers' consciousness about play materials' availability, ensuring equal possibilities for children's play in ECEC. Implications for policy and practice are discussed.
Effect of space allowance and mixing on growth performance and body lesions of grower-finisher pigs in pens with a single wet-dry feeder
Authors: Camp Montoro Jordi , Boyle Laura Ann , Solà-Oriol David , Muns Ramon , Gasa Josep , Garcia Manzanilla Edgar
Published: January 2021

Background

Low space allowance (SA) and mixing may result in reduced growth performance (GP) and animal welfare issues because of adverse social behaviours directed to pen mates. This could be exacerbated in pens with single space feeders owing to social facilitation of feeding behaviour. The present study aimed to investigate the effect of SA and mixing on GP and body lesions (BL) in pens with one single space wet-dry feeder.

Results

Two experiments were conducted on grower-finisher pigs from 10 to 21 weeks of age. In Exp1, pigs (N = 216) were assigned to three SA; 0.96 m2/pig (n = 6 pens; 10 pigs/pen; SA96), 0.84 m2/pig (n = 6; 12 pigs/pen; SA84) and 0.72 m2/pig (n = 6; 14 pigs/pen; SA72), in a randomized design. In Exp2, pigs (N = 230) were used in a 2 × 2 factorial randomized design considering SA and mixing as treatments. Pigs were assigned to two SA; 0.96 m2/pig (n = 10 pens; 10 pigs/pen; SA96) and 0.78 m2/pig (n = 10; 13 pigs/pen; SA78) and were either mixed or not at the entry to the finishing facility. GP was not affected by SA (P > 0.05) in either experiment. In Exp2, non-mixed pigs were 5.4 kg heavier (P <  0.001), gained 74 g more per day (P = 0.004), consumed 101.8 g more of feed per day (P = 0.007) and tended to have higher feed efficiency (P = 0.079) than mixed pigs from 11 to 21 weeks of age. Number of BL was affected by SA in both experiments. In Exp1, SA72 pigs had 74.4 and 97.4% more BL than SA96 and SA84 pigs at 20 weeks of age respectively (P <  0.01). In Exp2, SA78 pigs had 48.6, 43.6 and 101.3% more BL than SA96 pigs at 12, 16 and 21 weeks of age respectively (P <  0.05). Mixing did not affect the number of BL from 12 to 21 weeks of age in Exp2 (P > 0.05).

Conclusion

Mixing had a considerable effect on growth performance thus, strategies to avoid or mitigate mixing should be considered. Although space allowance had no effect on growth performance, high number of body lesions in the lower space allowance indicates that space allowances equal or below 0.78 m2/pig are detrimental to the welfare of pigs despite following the EU legislation.
A pilot study of a nurse-led integrated care review (the INCLUDE review) for people with inflammatory rheumatological conditions in primary care: feasibility study findings
Authors: Hider Samantha L. , Bucknall Milica , Jinks Clare , Cooke Kelly , Cooke Kendra , Desilva Erandie Ediriweera , Finney Andrew G. , Healey Emma L. , Herron Daniel , Machin Annabelle R. , Mallen Christian D. , Wathall Simon , Chew-Graham Carolyn A.
Published: January 2021

Background

People with inflammatory rheumatological conditions such as rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, polymyalgia rheumatica and giant cell arteritis are at an increased risk of common comorbidities including cardiovascular disease, osteoporosis and mood problems, leading to increased morbidity and mortality. Identifying and treating these problems could lead to improved patient quality of life and outcomes. Despite these risks being well-established, patients currently are not systematically targeted for management interventions for these morbidities. This study aimed to assess the feasibility of conducting a randomised controlled trial (RCT) of a nurse-led integrated care review in primary care to identify and manage these morbidities.

Methods

A pilot cluster RCT was delivered across four UK general practices. Patients with a diagnostic Read code for one of the inflammatory rheumatological conditions of interest were recruited by post. In intervention practices (n = 2), eligible patients were invited to attend the INCLUDE review. Outcome measures included health-related quality of life (EQ-5D-5L), patient activation, self-efficacy and treatment burden. A sample (n = 24) of INCLUDE review consultations were audio-recorded and assessed against a fidelity checklist.

Results

453/789 (57%) patients responded to the invitation, although 114/453 (25%) were excluded as they either did not fulfil eligibility criteria or failed to provide full written consent. In the intervention practices, uptake of the INCLUDE review was high at 72%. Retention at 3 and 6 months both reached pre-specified success criteria. Participants in intervention practices had more primary care contacts than controls (mean 29 vs 22) over the 12 months, with higher prescribing of all relevant medication classes in participants in intervention practices, particularly so for osteoporosis medication (baseline 29% vs 12 month 46%). The intervention was delivered with fidelity, although potential areas for improvement were identified.

Conclusions

The findings of this pilot study suggest it is feasible to deliver an RCT of the nurse-led integrated care (INCLUDE) review in primary care. A significant morbidity burden was identified. Early results suggest the INCLUDE review was associated with changes in practice. Lessons have been learnt around Read codes for patient identification and refining the nurse training.

Trial registration

ISRCTN, ISRCTN12765345
Regime specific spillover across cryptocurrencies and the role of COVID-19
Authors: Shahzad Syed Jawad Hussain , Bouri Elie , Kang Sang Hoon , Saeed Tareq
Published: January 2021

The aim of this study is to examine the daily return spillover among 18 cryptocurrencies under low and high volatility regimes, while considering three pricing factors and the effect of the COVID-19 outbreak. To do so, we apply a Markov regime-switching (MS) vector autoregressive with exogenous variables (VARX) model to a daily dataset from 25-July-2016 to 1-April-2020. The results indicate various patterns of spillover in high and low volatility regimes, especially during the COVID-19 outbreak. The total spillover index varies with time and abruptly intensifies following the outbreak of COVID-19, especially in the high volatility regime. Notably, the network analysis reveals further evidence of much higher spillovers in the high volatility regime during the COVID-19 outbreak, which is consistent with the notion of contagion during stress periods.
Preliminary engagement of a patient advisory board of African American community members with type 2 diabetes in a peer-led medication adherence intervention
Authors: Shiyanbola Olayinka O. , Kaiser Betty L. , Thomas Gay R. , Tarfa Adati
Published: January 2021

Background

The Peers Supporting Health Literacy, Self-efficacy, Self-Advocacy, and Adherence (Peers LEAD) program is a culturally tailored educational-behavioral 8-week intervention that addressed psychosocial and sociocultural barriers to diabetes medication adherence in African Americans. A brief 3-week version of the Peers LEAD intervention used a community engagement approach to examine the feasibility and acceptability of the intervention amongst patient stakeholders.

Main body

African Americans who were adherent to their diabetes medicines were paired with those who were non-adherent to their medicines. Together, they participated in the group and phone-based medication adherence intervention. Input from this brief intervention was important for the design of the remainder weeks of the 8-week program. The intervention targeted negative beliefs about diabetes, use of diabetes medicines, and offering culturally tailored peer support to improve medication adherence in African Americans. To receive input in the development and implementation of the program, we worked with community advisors and a peer ambassador board of African Americans who were adherent to their diabetes medicines. The peer ambassador board and community advisors reviewed intervention materials to ensure they were understandable and appropriate for the community. As well, they provided feedback on the process for intervention delivery.

Conclusion

The active engagement of the peer ambassador board and community advisors led to a revised intervention process and materials for a medication adherence program for African Americans with type 2 diabetes.
In vivo optical endomicroscopy: two decades of translational research towards next generation diagnosis of eosinophilic esophagitis
Authors: Wartak Andreas , Garber John G. , Yuan Qian , Shreffler Wayne G. , Hesterberg Paul E. , Katz Aubrey J. , Osman Hany , Leung Hui Min , Gao Anna , Otuya David O. , Grant Catriona , Gardecki Joseph A. , Nishioka Norman , Tearney Guillermo J. , Apiou-Sbirlea Gabriela
Published: January 2021

Histopathologic analysis of biopsy specimens obtained via white light endoscopy (WLE) is the gold standard for the diagnosis of several mucosal diseases in the upper gastrointestinal (GI) tract. However, this standard of care entails a series of critical shortcomings such as missing depth information, high costs, time inefficiency, low-resolution imaging in vivo, high sampling variability, missing intrinsic tissue-specific contrast, and anesthesia related risk. In the quest for a diagnostic technology to replace the current standard of care, in vivo optical endomicroscopy has emerged as a promising alternative. This paper tells the story of a cluster of optical microscopy-based modalities invented, further developed, or first-validated in the laboratory of Dr. Guillermo J. Tearney (Tearney Lab) at the Wellman Center for Photomedicine of Massachusetts General Hospital over the past two decades, that combined lead to a novel method for diagnosis of eosinophilic esophagitis (EoE). Rather than being a comprehensive literature review, this paper aims to describe the translational journey towards a disease specific diagnostic and research tool for this increasingly recognized yet poorly understood immune-mediated disorder of the esophagus.
Effects of soil water content and light intensity on the growth of Molinia japonica in montane wetlands in South Korea
Authors: Choi Yu Seong , Park Hyun Jun , Kim Jae Geun
Published: January 2021

Background

Montane wetlands are unique wetland ecosystems with distinct physicochemical characteristics, and Molinia japonica often makes dominant communities in montane wetlands in South Korea. In order to figure out the environmental characteristics of M. japonica habitats and the major factors for the growth of M. japonica, field surveys were conducted in five wetlands from September to October 2019. Also, soil was collected at every quadrats installed in surveyed wetlands to analyze the physicochemical features.

Results

The relative coverage of M. japonica was higher in low latitude wetlands than in high latitude. Redundancy analysis showed that soil water content had the strongest effect on the growth of M. japonica (F = 23.0, p < 0.001). Soil water content, loss on ignition, and relative light intensity showed a high correlation with the density (R = 0.568, 0.550, 0.547, respectively, p < 0.01) and the coverage of M. japonica (R = 0.495, 0.385, 0.514, respectively, p < 0.01). Soil water content, loss on ignition, and pH were highly correlated with each other.

Conclusions

Molinia japonica lives in acidic wetlands at high altitude in temperate zone of low latitude, with peat layer placed on the floor. Also, M. japonica prefers open spaces to secure enough light for photosynthesis. High shoot production of M. japonica resulted in adding new peat material in every year, and this layer enforces the environmental characteristics of M. japonica habitats. This study may provide insights for further understanding of the method how wetlands maintain acidic condition by itself in montane wetlands in temperate zone.
Transpedal approach for femoral-popliteal chronic total occlusions using the outback® elite re-entry device
Authors: Gorenchtein Mike , Rajper Naveed , Green Philip , Khullar Pankaj , Amoruso Daniel , Bulacan Christian Franz , Kwan Tak , Puma Joseph , Ratcliffe Justin
Published: January 2021

Background

Transpedal access is increasingly utilized for the treatment of peripheral artery disease (PAD). Femoral-popliteal artery chronic total occlusions (CTOs) are some of the most difficult lesion subsets that sometimes require the use of re-entry support devices during percutaneous intervention. Limited data is available on the use of re-entry devices when treating femoral-popliteal CTOs via transpedal access. The aim of this study was to demonstrate the feasibility of using the Outback® Elite re-entry device for the treatment of femoral-popliteal CTOs via the transpedal approach in an outpatient based lab setting.

Methods

Seventeen patients presented with femoral-popliteal CTOs in which treatment required the use of the Outback® Elite re-entry device. All procedures were performed in a single outpatient based lab. Patients were followed at 1 week and 1 month post-procedure, with lower extremity arterial duplex ultrasound assessment during the 1 month follow-up.

Results

The average patient age was 78 years-old, with 71% being males. Most patients presented with Rutherford class IV symptoms. Procedural success was achieved in all patients with no requirement to convert to femoral artery access in any of the cases. No immediate post-procedural complications nor at any time during follow-up were observed. Ultrasonography at 1 month follow-up showed patent intervention sites and access site vessels in all patients.

Conclusion

The use of the Outback® Elite re-entry device for the treatment of femoral-popliteal CTOs via transpedal access is a feasible option and may have potential benefits by avoiding risks associated with traditional femoral artery access.
Physiological response of sweet corn (Zea mays Ls.) grown under sandy soil to α-tocopherol treatments and different irrigation systems
Authors: Badr Elham Abd Elmoneim , Sadak Mervat Shamoon , Bakhoum Gehan Shaker , Khedr Howida Hassan Ahmed
Published: January 2021

Background

Antioxidants or vitamins are organic compounds that are needed in tiny quantities and have stimulatory role on growth and productivity of different plants. Two field experiments were conducted in two summer seasons to investigate the role of two irrigation systems (spraying and dripping) and α-tocopherol foliar treatment with different concentrations (0, 100 and 200 mg/L) on some growth indices and physiological aspects, yield and its components of sweet corn plants.

Results

The results show that dripping irrigation system was more effective than Sprinkler system on increasing the studied growth indices and biochemical attributes as well as yield quantity and quality. The results also show the enhancing effect of different α-tocopherol treatments on growth indices, and yield quantity and quality of the sweet corn varieties via enhancing photosynthetic pigments, indole acetic acid, phenolics, carbohydrates constituents, free amino acids and proline contents. Moreover, the interaction between the two irrigation systems and different concentrations α-tocopherol showed that, dripping irrigation with 200 mg/L α-tocopherol was the most effective treatment on increasing growth, yield and yield components of sweet corn plants.

Conclusion

It could be concluded that dripping irrigation system was more effective than Sprinkler system on increasing the studied growth indices and physiological aspects, yield and its constituents. Moreover, treatment of maize plant with α-tocopherol could improve the studied growth indices, physiological aspects and consequently yield.
A renewable energy forecasting and control approach to secured edge-level efficiency in a distributed micro-grid
Authors: Anaadumba Raphael , Liu Qi , Marah Bockarie Daniel , Nakoty Francis Mawuli , Liu Xiaodong , Zhang Yonghong
Published: January 2021

Energy forecasting using Renewable energy sources (RESs) is gradually gaining weight in the research field due to the benefits it presents to the modern-day environment. Not only does energy forecasting using renewable energy sources help mitigate the greenhouse effect, it also helps to conserve energy for future use. Over the years, several methods for energy forecasting have been proposed, all of which were more concerned with the accuracy of the prediction models with little or no considerations to the operating environment. This research, however, proposes the uses of Deep Neural Network (DNN) for energy forecasting on mobile devices at the edge of the network. This ensures low latency and communication overhead for all energy forecasting operations since they are carried out at the network periphery. Nevertheless, the cloud would be used as a support for the mobile devices by providing permanent storage for the locally generated data and a platform for offloading resource-intensive computations that exceed the capabilities of the local mobile devices as well as security for them. Electrical network topology was proposed which allows seamless incorporation of multiple RESs into the distributed renewable energy source (D-RES) network. Moreover, a novel grid control algorithm that uses the forecasting model to administer a well-coordinated and effective control for renewable energy sources (RESs) in the electrical network is designed. The electrical network was simulated with two RESs and a DNN model was used to create a forecasting model for the simulated network. The model was trained using a dataset from a solar power generation company in Belgium (elis) and was experimented with a different number of layers to determine the optimum architecture for performing the forecasting operations. The performance of each architecture was evaluated using the mean square error (MSE) and the r-square.