bluuebunny/crossref_metadata_2025 huggingface dataset:
Identidad rural: discursos e imágenes en pequeñas localidades de Paysandú, Uruguay
Authors: Vicci Gonzalo , Cabrera Mónica
Published: February 2022

Este artículo tiene como objetivo exponer las líneas centrales de un proyecto de trabajo que se propone identificar y analizar participativamente el impacto que generan los discursos normalizados en relación a la ruralidad en el entorno de las pequeñas comunidades rurales del interior del departamento de Paysandú, Uruguay. El contexto de generalización de acceso a conexión a Internet y dispositivos de información y comunicación en todo el Uruguay podría ser desencadenante de modificaciones en la forma en la que los niños en edad escolar que residen en el medio rural están construyendo sus identidades. Interesa poner atención en los condensados visuales que circulan, si vienen de lo tradicional o el entorno familiar o descartar el supuesto de hibridación que desencadena la globalización, identificando los mecanismos a través de los cuales se conforman esas identidades de la ruralidad y el vínculo con los espacios comunes del entorno. Se propone tomar como centro las escuelas rurales unidocentes y multigrado, considerando que constituyen y definen un espacio público de construcción de ciudadanía en un territorio específico y seleccionado. Si bien estaba prevista la implementación de este dispositivo, la irrupción de la pandemia frenó la posibilidad de concretar las actividades tal cual se estaban diseñadas, lo cual modificó el proceso y abrió el desafío de explorar nuevas formas de aproximación. Sobre ese proceso da cuenta la presente comunicación.
L’autre ment? D’une autre mode? Vérités et mensonges du pédagogique
Authors: Chalmel Loïc
Published: February 2022

Ce texte met en perspective l’idée de l’AUTRE sous quatre dimensions de la pensée humaine : théologique, philosophique, psychologique et pédagogique. En ce qui concerne la première, nous ferons appel aux idées d’Erasme et de Luther sur le « libre arbitre ». Pour la seconde, nous convoquerons les idées de Rousseau et de Kant sur l’« autonomie ». Quant à la troisième, c’est Freud et son concept de « transfert » qui seront mis à contribution. Enfin, en ce qui a trait à la dimension pédagogique, nous nous appuierons sur Comenius, Pestalozzi, Steiner, Korczak et Freire pour aborder la relation entre pratiques et théories dans les contextes et les univers de référence. Pour penser cette dimension pédagogique, les auteurs des autres dimensions seront considérés comme « héritage » ou « testament », et nous nous poserons la question de savoir comment ils sont arrivés jusqu’à nous pour demeurer présents dans nos réflexions actuelles sur l’éducation.
Breeding behaviour of a pair of Spotted Harriers Circus assimilis near Tamworth, New South Wales
Authors:   Zoology, University of New England, Armidale NSW 2351, Australia, Debus S.J.S , Dunne J.E. , Hosking J.P. , Jones K. , Kane D.A. , Kane C.J. , Mitchell G.I. , Stewart T.
Published: February 2022

A pair of breeding Spotted Harriers Circus assimilis was observed in an agricultural environment near Tamworth, New South Wales, in winter–spring 2020 for 290 hours over 68 days from pre-laying and incubation to the post-fledging period. Laying occurred at the end of June, hatching at the beginning of August and fledging in mid September. Only the female incubated and brooded, and the male supplied all prey from the incubation period to late in Week 3 of the nestling period, after which the female contributed. Two chicks fledged of three that hatched; there was some sibling competition for food. The incubation period lasted 34 ± 1 days, and the nestling period 43 days for the older (female) chick and c. 40 days for the younger (male) chick. Prey consisted of ground-dwelling small mammals, birds and reptiles that are typical of agricultural land. The male’s prey-delivery rate to the incubating female was 0.22 item/h. Overall, the male delivered 0.31 item/h and the female 0.09 item/h to the nestlings, for a combined average of 0.4 item/h, peaking in the pre-fledging fortnight. The combined rate in the post-fledging period was 0.39 item/h (Week 1), declining to 0.16 item/h to the fledglings in Week 2 when only the male delivered prey and the adult female consumed some of his catch. Estimated biomass delivered ranged between ~20 and ~100 g/item (often 20–50 g/item). Breeding behaviour and nestling/fledgling growth and development are described. The juveniles appeared to be dependent or partly so for at least 3 weeks, and apparently roosted in the nest area in Week 4, after which they could not be located.
Symmetry of Galactic Structure
Authors: Oldani Richard
Published: February 2022

A fully relativistic description of an atomic clock is achieved by applying the equivalence principle directly to the space-time coordinates of electron transitions. The resulting differential equation of motion is then reformulated by means of Hamilton's principle to obtain an equivalent integral equation. Generalized coordinates describe the electron in a configuration space consisting of three coordinates that define the origin, or nucleus, and three coordinates that define the manifold on which the electron is constrained to move (the electron shells). Following Dirac the particle model is transformed to a field model by describing energy quantization as the four-dimensional localization of radial and transverse electromagnetic fields. It can then be compared to the emission energy of galaxies in terms of the radial baryonic fields and transverse gravitomagnetic fields that emanate from black holes. The symmetry exhibited by these diverse forms of energy demonstrates that the properties of energy, when described four-dimensionally with time, are independent of the material system which supports them.
Portable FRET-Based Biosensor Device for On-Site Lead Detections
Authors: Lai Wei-Qun , Chang Yu-Fen , Chou Fang-Ning , Yang De-Ming
Published: February 2022

Most methods for measuring environmental lead (Pb) content are time consuming, expensive, hazardous, and restricted to specific analytical systems. To provide a facile, safe tool to detect Pb, we created pMet-lead, a portable fluorescence resonance energy transfer (FRET)-based Pb biosensor. pMet-lead comprises a 3D-printed frame housing a 405-nm laser diode — an excitation source for fluorescence emission images (YFP and CFP) — accompanied by optical filters, a customized sample holder with a Met-lead 1.44 M1 (the most recent version)-embedded biochip, and an optical lens aligned for smartphone compatibility. Measuring the emission ratios (Y/C) of the FRET component enables Pb detection with a dynamic range of nearly 2 (1.96), pMet-lead/Pb dissociation constant (Kd) 45.62 nM, and limit of detection 40 nM (0.832 μg/dL, 8.32 ppb). To mitigate earlier problems with lack of selectivity for Pb vs. zinc, we preincubated samples with tricine, a low-affinity zinc chelator. We validated pMet-lead measurements of characterized laboratory samples and unknown samples from six regions in Taiwan by inductively coupled plasma mass spectrometry (ICP-MS). Notably, two unknowns had Y/C ratios significantly higher than that of the control (3.48 ± 0.08 and 3.74 ± 0.12 vs. 2.79 ± 0.02), along with Pb concentrations (10.6 ppb and 15.24 ppb) above the WHO-permitted level of 10 ppb in tap water, while the rest four unknowns showing no detectable Pb upon ICP-MS. These results demonstrate that pMet-lead provides a rapid, sensitive means for on-site Pb detection in water from the environment and in living/drinking supply systems to prevent potential Pb poisoning.
The Prevalence of Onchocerciasis-Associated Epilepsy in Mundri West and East Counties, South Sudan: A Door to Door Survey
Authors: Colebunders Robert , Jada Stephen Raimon , Dusabimana Alfred , Abd-Elfarag Gasim , Okaro Samuel , Brusselaers Nele , Carter Jane Y , Logora Makoy Ybi , Rovarini Jacopo Mattia
Published: February 2022

A two phase survey of epilepsy was conducted in selected villages in Mundri West and East Counties (26 June−8 July, 2021), an onchocerciasis-endemic area in Western Equatoria State in South Sudan. In the first phase, households were visited by a trained research team to identify persons suspected to have epilepsy. In the second phase, persons suspected to have epilepsy were interviewed and examined by a clinician to confirm the diagnosis. A total of 364 households agreed to participate in the survey, amounting to 2,588 individuals. The epilepsy screening questionnaire identified 91 (3.5%) persons suspected to have epilepsy, of whom in 86 (94.5%) the diagnosis of epilepsy was confirmed by a clinician. The overall prevalence of confirmed epilepsy was 3.3% (95% CI: 2.7-4.1%), and of nodding syndrome 0.9% (95% CI: 0.6-1.4%). In 61 (16.8%) households there was at least one person with epilepsy. Only 1,212 (46.9%) of 2,583 people took ivermectin during the last distribution round in 2021. The annual epilepsy incidence was 77.3/100.000 (95% CI: 9.4-278.9/100,000) and the annual epilepsy mortality 251.2/100,000 (95% CI: 133.8-428.7/100,000). In conclusion, a high prevalence and incidence of epilepsy was observed in villages in Mundri. Urgent action is needed to prevent children from developing onchocerciasis-associated epilepsy by strengthening the local onchocerciasis elimination programme.
Feasibility of an Open-Source Algorithm for Predicting Sea Surface Temperature Based on Three Multi-Resolution Data Sources
Authors: Rodríguez-Sobreyra Ranulfo , Álvarez-Sánchez León Felipe , Flores-De-Santiago Francisco
Published: February 2022

The quantification of sea surface temperature (SST) through space platforms has revolutionized how we obtain information at a global level. However, the main disadvantage of obtaining SST with satellite images consists of its inherent coarse spatial resolution. One solution could be the use of downscaling algorithms to create sequences of matrices at a higher resolution. We used the same SST data source from the MODIS-Aqua sensor at three spatial resolutions of 9 km, 4.5 km, and 1 km in the Gulf of California. Based on an open-source algorithm, the original SST images were downscaled to 4.5 km, 1 km, 500 m, 250 m, and 125 m per pixel scales. Results indicate a strong linear relationship between the original SST-MODIS data and the modeled data for all spatial resolutions. This study demonstrates the feasibility of an open-source downscaling algorithm to enhance the spatial resolution of SST images in a marginal sea.
The Italian Alpine and Subalpine Trouts. Taxonomy, Evolution, and Conservation
Authors: Polgar Gianluca , Iaia Mattia , Righi Tommaso , Volta Pietro
Published: February 2022

During the last 150 years, the trout-culture industry focused on enhancing trout populations by stocking, in response to the growing anglers’ demand and the habitat degradation associated to the rapid urbanization and hydropower development. The industrialized north of Italy, home to the Italian Alpine and subalpine trout populations, is the source of most of the revenues of the national trout-culture industry. Its rapid growth and the massive introduction of non-native interfertile trouts eroded the genetic diversity of native lineages, leading to harsh confrontations between scientists, institutions, and sportfishing associations. We review here the state of art of the taxonomy and distribution of the northern Italian native trouts, presenting both scientific results and historical documentation. We think the only native trouts in this region are Salmo marmoratus, widespread in this region, plus small and fragmented populations of S. ghigii, present only in the Southwestern Alps. We strongly recommend the interruption of stocking of domesticated interfertile non-native trouts in this area, and recommend the adoption of Evolutionary Significant Units for salmonid fishery management. We further propose future research directions for a sustainable approach to the conservation and ecosystem management of the fishery resources and inland waters of northern Italy.
A Case of Focal Segmental Glomerulosclerosis in a Young Girl with a Very Low Birth Weight
Authors: Kashiwagi Yasuyo , Agata Kazushi , Yamanaka Gaku , Kawashima Hisashi
Published: February 2022

In Japan, the prevalence of low birth weight (LBW) has been estimated to be approximately 10%, which is the highest among developed countries. This high prevalence might affect the prevalence of LBW-associated diseases in the adult population of Japan. Recently, LBW has been recognized as a contributing factor to post-adaptive focal segmental glomerulosclerosis (FSGS) in adulthood; however, few reports to date have evaluated the clinical and pathological characteristics of post-adaptive FSGS. A 13-year-old girl was referred to our hospital owing to mild proteinuria, which was detected at a school urinary screening. She was born at a gestational age of 23 weeks, with a very LBW of 630 g. Dipstick urinalysis revealed grade (2+) proteinuria. Her serum creatinine level was 1.02 mg/dL, and she was diagnosed as having stage 2 chronic kidney disease (CKD). Her serum uric acid level was 7 mg/dL. Her mother and 16-years old brother had hyperuricemia, too. A percutaneous renal biopsy leads to a diagnosis of FSGS. After 3 years of treatment with an angiotensin receptor blocker, her proteinuria decreased. However, her serum creatinine level was 1.07 mg/dL, and she still had stage 2 CKD. We considered that in this patient, the first hit was her LBW, and the second hit was hyperuricemia. The second hit might be associated with the development of CKD. The birth history of patients is not usually confirmed by nephrologists. Our case demonstrates that obtaining information regarding the preterm birth and LBW of patients is important in the diagnosis of noncommunicable diseases, because school urinary screening is not routinely performed in countries other than Japan.
Structural Inequities, HIV Community-Based Organizations, and the End of the HIV Epidemic
Authors: Robillard Alyssa G. , Julious Carmen H. , Smallwood Stacy W. , Douglas Mark , Gaddist Bambi W. , Singleton Tyler
Published: February 2022

Community-based organizations (CBOs) are integral to achieving the goal of Ending the HIV epidemic (EHE). Their familiarity with and proximity to communities position them to effectively implement strategies necessary to address determinants of health through their formal and informal medical and social services. However, structural inequities have contributed to the demise of many organizations that were instrumental in early responses to the HIV epidemic.

We define structural inequities for HIV CBOs as systems in which policies, institutional practices, organizational (mis)representations, and other norms work to produce and maintain inequities that affect CBOs’ ability to survive and thrive. In this discussion, we describe the organizational threats to grassroots HIV CBOs and the risks to livelihood and longevity, including examples.

The invaluable role of HIV CBOs in EHE and their role in responding to existing and novel infectious diseases like COVID-19 should not be overlooked. Recommendations to promote structural equity are offered. (Am J Public Health. 2022;112(3):417–425. https://doi.org/10.2105/AJPH.2021.306688 )
The Effects of Cassava Pomace and Protected Soybean Meal on Dairy Milk Production and Quality
Authors: Sabastian Basofi , Widyobroto Budi Prasetyo , Astuti Andriyani
Published: February 2022

The objective of the study was to determine the effect of cassava pomace and protected soybean meal on dairy milk production and quality of mid lactating dairy cows. This research was conducted at Turen, Malang from January to April 2020. Twelve lactating Friesian Holstein dairy cows were divided into 2 groups so that each group consisted of 6, namely the control and treatment groups. The feed were a combination of forage and concentrate with a ratio of 35:65 in dry matter (DM). The control group received wet concentrate with DM content of 30.58%, 14.87% CP, and 75.06% TDN, while the treatment groups received concentrate with supplementation of cassava pomace 15% of DM ratio and protected soybean meal 45 g/l milk production. The variables observed were nutrient consumption, milk production and quality. Data between the two groups were analyzed using the Independent Sample T-test. The results showed that the addition of waste cassava and protected soybean meal increased (P<0.05) nutrient consumption (DM, OM, CP, CF, EE, and TDN). Milk production in the treatment group was higher (P<0.05) than control group (9.46 vs. 6.07 Ls/head/day). Milk protein production in the treatment group was higher (P<0.05) than control group (0.37 vs. 0.21 L/head/day). The content of milk protein and milk fat between the control and treatment groups was not significantly different (respectively 3.19 Vs 3.28; 4.46 vs 4.42 %). Milk protein and fat production in the treatment group was higher (P<0.05) than control group (0.21 vs 0.37; and 0.50 vs. 0.30 L/head/day respectively). The composition of glucose and blood urea in control and treatment dairy cows were not significantly different. In conclusion, giving cassava pomace and protected soybean meal to dairy cows during mid lactation increased nutrient consumption, milk production, milk protein production, and milk fat production but did not increase percentage of milk protein and fat. The treatment also did not affect blood glucose and nitrogen urea contents.
The Effect of Ensiled Maize Stover Combined with Additives as an Animal Feed
Authors: Rohmah Binti Nur , Hartutik Hartutik , Marjuki Marjuki
Published: February 2022

The research purpose was to determine the effect of addition starter Pediococcus pentosaceus with different level in the maize stover silage on the in vitro digestibility, fermentation product, and microbial protein synthesis. The treatments consist of T1 = maize stover silage + molasses 10%, T2 = maize stover silage + molasses 10% + P. pentosaceus (1x105 cfu/g), and T3 = maize stover silage + molasses 10% + P. pentosaceus (1x106 cfu/g), fermented for 0 and 21 days. The research method was an experiment by using Randomized Block Design (RBD), with 3 treatments and 4 blocks. The result showed that addition of starter P. pentosaceus in the maize stover silage gave significant effect (P<0.05) on dry matter (T1 (65.67±1.56%), T2 (65.78±0.60%), and T3 (67.54±1.11%)), and organic matter digestibility (T1 (66.93±0.48%), T2 (68.15±1.34%), T3 (68.68±1.04%)) at 21 days incubation. The result of statistical analysis showed that treatment gave significant effect (P<0.05) on gas production at 96 hours of silage with 0 day incubation, T1 (114.74±0.60 ml/500mg DM), T2 (116.12±1.44 ml/500mg DM), T3 (116.97±1.12 ml/500mg DM) and 21 days incubation, T1 (124.27±0.59 ml/500mg DM), T2 (125.27±1.49 ml/500mg DM), and T3 (126.16±1.26 ml/500mg DM). All treatments not gave significant effect (P˃0.05) on degradability, NH3 and microbial protein synthesis on 0 days incubation. However, did it gave significantly effect (P˂0.05) in 21 days incubation silage, dry matter degradability (T1 (66.16±0.42%), T2 (65.70±0.58%), T3 (66.66±0.21%)), organic matter degradability (T1 (65.67±1.56%), T2 (65.78±0.60%), and T3 (67.54±1.11%)), microbial protein synthesis (T1 (37.72±0.13 g/N/kg OM apparently fermented), T2 (37.84±0.64 g/N/kg OM apparently fermented), T3 (38.82±0.56 g/N/kg OM apparently fermented). It can be concluded that addition of starter P. pentosaceus (1x106 cfu/g) on maize stover silage with 21 days incubation can produce silage with good quality on the in vitro digestibility, fermentation product, and microbial protein synthesis.
Comparison of 2 Patient-Reported Outcome Measures in Brachial Plexus Birth Injury
Authors: Vuillermin Carley , Eisenberg Katherine , Williams David , Tartarilla Ashley B. , Bauer Andrea , Waters Peter M.
Published: February 2022

Background:

Patient-reported outcome (PRO) measures are integral to clinical practice. While new instruments bring updated methodology and delivery methods, they must be compared with existing measures to ensure that they are comparable with currently used instruments and allow ongoing comparison. In this study, we evaluated 2 PRO measures for patients with brachial plexus birth injury (BPBI): the legacy Pediatric Outcomes Data Collection Instrument (PODCI) and the newer Patient-Reported Outcomes Measurement Information System (PROMIS). We hypothesized that the PROMIS and PODCI would be correlated, with high convergent validity, and that PROMIS assessment would have higher discriminant validity and take less time to complete than the PODCI.

Methods:

A cross-sectional prospective study was undertaken at a tertiary referral center. Subjects (n = 50) completed the PODCI and PROMIS, both as short form (SF) and computer-adaptive testing (CAT) measures, in randomized order. Patient and parent questionnaires were completed according to subject age. Physicians completed upper-extremity (UE) physical function tests. Correlations between PODCI and PROMIS scores were analyzed to determine convergent validity, UE subscores were analyzed relative to physical function scores to assess discriminant validity, and completion times were calculated to determine survey time to completion.

Results:

High convergent validity was found between the parent-reported PODCI, PROMIS SF, and PROMIS CAT measures. A ceiling effect was found for the PODCI but not for the PROMIS. The PROMIS CAT had better discriminant validity than either the PROMIS SF or PODCI. Patient and parent outcome scores did not highly correlate. Survey time to completion varied by PRO measure and method of delivery.

Conclusions:

There was high convergent validity between the UE domains of the PROMIS and PODCI, with a lower time to completion for the PROMIS SF. Although results show that the PROMIS CAT may take longer to complete, there are notable benefits to its adoption, including high convergent validity with the PODCI and better discriminant validity than the PODCI and PROMIS SF measures.
Proliferative effect of aqueous extract of sea cucumber (Holothuria parva) body wall on human umbilical cord mesenchymal stromal/stem cells
Authors: Rasekh Poorya , Kameli Ali , Khoradmehr Arezoo , Baghban Neda , Mohebbi Gholamhossein , Barmak Alireza , Nabipour Iraj , Azari Hossein , Heidari Yaser , Daneshi Adel , Bargahi Afshar , Khodabandeh Zahra , Zare Shahrokh , Shirazi Reza , Almasi-turk Sahar , Tamadon Amin
Published: February 2022

Background: The sea cucumber potentials for stem cell proliferation induction and their mechanisms of bioactive compounds in its extract have been studied. Human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to aqueous extract of Holothuria parva body wall. Methods: Using GC-MS analysis on aqueous extract of H. parva, proliferative molecules were detected. The extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were used. MTT proliferation, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, effects of aqueous extract of H. parva and EGF on cell proliferation markers were detected. Computational modeling done to detect effective proliferative compounds in aqueous extract of H. parva. Results: MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of H. parva had proliferative effects on hUC-MSCs. Count of the cells treated with the 20 µg/mL of the extract was increased faster and higher than the control group (P<0.05). This concentration of extract did not have significant effects on hUC-MSCs viability. The cell cycle assay of hUC-MSCs showed that percent of cells in the G2 stage of the extract was biologically higher than the control group (P>0.05). Expression of the cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT were increased comparing with the control group. Moreover, expression of the p21 and PCNA decreased after treating hUC-MSCs with the extract. However, the CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of the cdk-4 and cdk-6 was decreased after treatment with the extract. Between the detected compounds, 1-methyl-4-(1-methylethenyl)-benzene showed better affinity to cdk-4 and p21 than tetradecanoic acid. Conclusions: The H. parva aqueous extract showed proliferative potential on the hUC-MSCs.
Upregulation of CCT3 Predicts Poor Prognosis and Promotes Cell Proliferation via Inhibition of Ferroptosis and Activation of EGFR Signaling in Lung Cancer
Authors: Wang Kun , He Jian , Tu Changling , Xu Hui , Zhang Xugang , Lv Yongchang , Song Chao
Published: February 2022

Background
Chaperonin containing TCP1 subunit 3 (CCT3) acts as an oncogene in cancers, whereas its role in lung cancer is poorly understood. This study investigated the clinical relevance and function of CCT3 in lung cancer.
Methods
Clinical relevance of CCT3 in lung cancer was analyzed based on TCGA database. qRT-PCR and Western blot assay were used to detect mRNA and protein expression, respectively. CCK8 and colony formation were performed to measure cell viability. Migration was detected by transwell. PI and PI/Annexin V-FITC assay kit were used to determine cell cycle and apoptosis, respectively. Luciferase activity was performed to check the whether CCT3 regulated slc7a11’s transcription activity. Ferroptosis was determined by incubating the cells with ferroptosis inducer, inhibitor and apoptosis inhibitor, followed by cell viability examination.
Results
We found that CCT3 was overexpressed in lung cancer tissues. Overexpression of CCT3 predicted poor prognosis in the patients. Loss-of-function and gain-of-function experiments demonstrated that CCT3 promoted the proliferation and colony formation of lung cancer cells. In addition, CCT3 knockdown suppressed cell cycle progression, cell migration and induced slc7a11-mediated cell ferroptosis, but not apoptosis. We also found that CCT3 activated EGFR and AKT. Gefitinib significantly reduced the viability of CCT3 overexpressed lung cancer cells, while had minimal effect on the proliferation of control cells, suggesting that CCT3 dictates the sensitivity of lung cancer cells to EGFR inhibition.
Conclusion
Our study demonstrates that CCT3 contributes to the proliferation and growth of lung cancer cells through inhibition of ferroptosis and activation of EGFR and AKT.
Gene Expression and DNA Methylation in Human Papillomavirus Positive and Negative Head and Neck Squamous Cell Carcinomas.
Authors: Stanković Snežana , Rich April , Anayannis Nicole , Cabarcas-Petroski Stephanie , Schramm Laura , Meneses Patricio I.
Published: February 2022

High-risk human papillomaviruses (HPV) are an important agent that cause head and neck squamous cell carcinomas (HNSCC). Besides viral induced cancers, tobacco use and heavy alcohol consumption are believed to cause DNA damage, promoting tumorigenesis. How gene expression and DNA methylation differ between HNSCC based on HPV status is yet unknown. We used publicly available gene expression and DNA methylation data from the Cancer Genome Atlas and compared HPV positive and HPV negative HNSCC. We used differential gene expression analysis, differential methylation analysis and a combination of these two analyses to identify differences. Differential expression analysis identified 1854 differentially expressed genes including PCNA, TNFRSF14, TRAF1, TRAF2, BCL2, and BIRC3. SYCP2 was identified as one of the top deregulated genes in the differential methylation analysis and in the combined differential expression and methylation analysis. Additionally, pathway and ontology analysis identified the extracellular matrix and receptor interaction pathway to be the most altered between HPV negative and HPV positive HNSCC groups. Combining gene expression and DNA methylation can help elucidate genes involved in HPV positive HNSCC tumorigenesis such as SYCP2 and TAF7L.
Observational study: Application of Therapy with Equine Serum for Patients Diagnosed with COVID-19 in Tucumán, Argentina
Authors: Chahla Rossana Elena , Ruiz Luis Medina , Contreras Miguel Enrique Ferré , Raya Mario , MsC Natalia Herrera , Ortega Eugenia Silvana , Goroso Daniel Gustavo , de Bruno María de los Ángeles Peral
Published: February 2022

Purpose: The objective of this work is to make an observational study of the usage in moderate or severe state COVID-19 patients of a new therapeutic commercial product obtained after immunization of horses: CoviFab® ELEA F(ab')2 fragmented equine immunoglobulins anti SARS-CoV2.Methods: Participant Centres depend on the Public Health System of Tucumán, Argentina were recruitment. Subjects were assigned to the Moderate Patient Group (MPG) and the Severe Patient Group (SPG), classified according to WHO criteria.In total, n = 84 were enrolled for this study. The subjects were divided into MPG and SPG. All participants were evaluated by physical examination and COVID-19 infection was diagnosed with positive RT-PCR. Each subject received two doses of 0.16 ml / kg, according to the subject's body weight. A generalized linear model with binomial distribution was adjusted for the number of symptoms. Data was analysed using proportion, bivariate and logistic regression. P-value was considered significant at the p< 0.05 threshold.Results: Both groups were similar in age, sex, and comorbidities. A higher proportion of patient with medical discharge was observed in MPG (91.4%) vs. SPG (55.3%) (p= 0.004). MPG showed 9 times more chance of receiving medical discharge than SPG (9.33 CI= [1.65, 52.81]; p= 0.012). Then, the chance to get medical discharge was independent of variables sex, age, and comorbidities. Conclusions: Treatment with Equine Serum in patients with moderate and severe disease of COVID-19 managed to slightly reduce hospitalization time. This treatment improved the clinical state to obtain medical discharge. The bivariate analysis showed 8 times more chance in MGP versus SGP to receive of medical discharge and this chance was independent of the pre-existent comorbidities.
A visual knowledge map analysis of mine fire research based on Citespace
Authors: Wang Feiran , Tan Bo , Chen Yue , Fang Xiyang , Jia Guowei , Wang Haiyan , Cheng Gang , Shao Zhuangzhuang
Published: February 2022

Mine fire has always been a serious disaster in coal industry, many academic achievements have poured out in the past two decades for solving this problem. In this study, visual analysis was conducted to grasp the hotspots and development trend of mine fire research. Papers that published in 1999-2020 were retrieved as the data basis from Web of Science, and Citespace was used to carry out knowledge map analysis. The results shown that number of papers has increased steadily since 2005 and achieved explosive growth since 2014. DENG J is the first published author among many scholars. China, the US, Australia are active areas in mine fire research and China University of Mining and Technology ranks first in this field. The highest co-occurrence frequency key word is “spontaneous combustion”. "INTERNATIONAL JOURNAL OF COAL GEOLOGY" and "FUEL" provide guidance for mine fire research. Fire prevention technology, low carbon, ecology and sustainable development are the hot research in recent years.
Characterization Of The Complete Mitochondrial Genome Of Sea Duck Mergus Serrator And Comparison With Other Anseriformes Species
Authors: Peng CHEN , Jiaqi LI , LI Hongbo , LIU Wei , Jianliang ZHANG
Published: February 2022

Background: As a key group in the Anatidae, the Mergini tribe (sea ducks) is strongly structured, with clear genetic assignments and phylogenetic relationships. The tribes also differ in terms of breeding strategy, longevity, and bionomic strategy. The Mergini tribe as an excellent tribe to investigate molecular adaptions to elevate metabolic burden associated with other life-history characteristics. Mitochondrial genome (mitogenome) plays a critical role of molecular evolution and phylogenetic. Phylogenetic analyses can help investigate the relationships between mitochondrial DNA (mtDNA) molecular evolution and metabolic performance.Results: The mitogenome of Mergus serrator was 16,627 bp in length, and its structure was circular. Annotation of the mitogenome presented 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and 1 control region. To determine the intraspecific evolutionary relationships among Anseriformes, the mitogenomes of 53 other Anseriformes species were examined concurrently. Among Anseriformes species, the shortest mitogenome belongs to Oxyura jamaicensis (15,914 bp) and the longest is that of Anseranas semipalmata (16,868 bp). The mean base composition of the mitogenome of Anseriformes was T (22.31 ± 0.51%), C (32.63 ± 0.64%), A (29.36 ± 0.64%), and G (15.71 ± 0.52%), indicating a slight specific bias towards A and C. AT content ranged of the mitogenome was from 50.27% to 55.31%, with an average value of 51.67 ± 1.10%, higher than the GC content (Table 1) and similar to that of birds in general (50.5% to 57.7%).Conclusions: The structure and gene arrangement of the genome were basically the same as those of Anseriformes species. The analyses indicated that the Anseriformes include the families Anseranatidae, Anhimidae, and Anatidae. Furthermore, Anatinae is composed of Aythyini, Anatini, Somaterini, and Mergini. M serrator was sister to M. merganser and M. squamatus, and this group belongs to Mergini. The results of selection analysis demonstrated different evolutionary rates between Mergini tribes and other tribes, and most protein-coding genes evolved under purifying selection except for the ND6 gene.
Lens Iris Diaphragm Retropulsion Syndrome: incidence, risk factors and management. A prospective study.
Authors: Hallali Gabriel , AUBERT Tristan , Souied Eric , GLACET-BERNARD Agnès
Published: February 2022

Purpose: The lens-iris diaphragm retropulsion syndrome (LIDRS) corresponds to a brutal deepening of the anterior chamber during phacoemulsification. LIDRS is painful for the patient and sometimes causes intraoperative complications. This study was designed to assess the preoperative risk factors of LIDRS. Setting: Monocentric study in the university department of ophthalmology, Intercommunal Hospital Center, Creteil, France. Design: Prospective open-label observational study. Methods: Preoperative parameters of consecutive patients who underwent cataract surgery were recorded. LIDRS occurrence was evaluated during surgery and classified into 3 stages. Except for the first patient, a simple and rapid maneuver to separate the iris from the capsule (capsular touch) was performed in all patients with LIDRS. Results: LIDRS occurred in 101 out of 205 included eyes: mild in 73.2% (74/101) , marked in 16.8% (17/101) and severe in 9.9% (10/101). LIDRS eyes had a longer axial length (p<0.01) and a thinner lens than the control group (p<0.01). Univariate logistic regression showed that the main predictive factors of LIDRS were long axial length, decrease lens thickness. LIDRS occurred in 83% (5/6) of eyes with prior vitrectomy. Complications occurred (zonular dehiscence), especially in vitrectomized eyes. The capsular touch allowed in almost all eyes to restore normal chamber depth and to prevent complications. Conclusions: Myopia and prior vitrectomy were the main risk factors of LIDRS. The capsular touch allowed to resolve LIDRS in almost all eyes. Analysis of preoperative factors can help the surgeon to identify patients at risk and to prevent patient pain and intraoperative complications associated with LIDRS.
What Factors Contribute to Moral Problems in Patient Care? A Scoping Review and Case Series of Ethics Consultations at a Somatic and a Psychiatric University Hospital in Switzerland
Authors: Schürmann Jan , Reiter-Theil Stella
Published: February 2022

Background
Health care professionals (HCPs) are regularly exposed to moral problems arising in patient care that may compromise quality of care. An understanding of risk factors and early indicators may help practitioners to address these problems early on. This study aims to provide an overview of moral risk factors in patient care. Risk factors known from the literature and those that have led to moral problems discussed in clinical ethics consultations (CECs) are examined. Characteristics, ethical issues and outcomes of CEC are also presented.
Methods
Scoping review on moral risk factors in patient care; CEC case series with semi-structured analysis of documentation (consultation reports, feedback forms, electronic patient records) from 204 CECs held between 2012 and 2020 at a somatic (USB) and a psychiatric (UPK) university hospital in Basel, Switzerland.
Results
Ninety-nine moral risk factors were identified from the literature referring to four risk areas: patient (41), family (12), health care professional (29) and system (17). Eighty-seven of these were documented at least once in CEC. The most prevalent factors were patient vulnerability (100%), absent or unclear hospital policies (97.1%), shift work (83.3%), inadequate understanding between patient and HCPs (73.5%), inadequate communication (66.2%), disagreement between patient and HCPs about care (58.8%), and multiple care teams (53.4%). The prevalence differs significantly depending on the medical specialty. CECs are requested as often in psychiatry as in somatic medicine. In the USB, ethical questions about life-sustaining treatment (27.8%), maternal-foetal conflicts (18.8%) and assisted reproductive technology (10.5%) are the most prevalent; in the UPK, questions about coercive measures (41.8%), risk and opportunity assessment (14.9%) and child welfare endangerment (13.4%) predominate. Overall, a consensus was almost always reached among the participants (98.5%) and the results implemented (93.9%). CECs were rated as helpful (95.0%) or partly helpful (5.0%).
Conclusions
CEC is useful in both somatic medicine and psychiatry to find consensual and helpful solutions to moral conflicts. There are highly prevalent and potent moral risk factors in all dimensions relevant in clinical care that can be used to prevent moral problems. Further empirical research is needed to create different risk profiles specific to the medical specialities.
Observations of a magnetic dip in a quiescent prominence foot
Authors: Chen Huadong , Ma Suli , Su Yingna , Zhou Guiping , Priest Eric , Shen Yuandeng , Xia Chun , Tu Weining , Fletcher Lyndsay , Zhang Jun
Published: February 2022

Solar prominences (or filaments) are cooler and denser plasma suspended in the much hotter and rarefied solar corona. When viewed on the solar disc filament barbs or feet protrude laterally from filament spine. When viewed at solar limb, they extend down to the chromosphere. For a long time, the magnetic field orientation of barbs has remained a mystery due to the paradox that the barbs possess vertical fine structures and flows but are likely to be supported in a horizontal magnetic field. Here we present highly suggestive observations of a magnetic dip in a quiescent prominence foot with an upward-curved field. That is indicated by the horizontal bidirectional outflows probably produced by magnetic reconnection between the fields of a tiny erupting filament and those in a prominence foot. The observations shed light on the field structure of prominences which is crucial for the instability that accounts for the eruption of prominences and coronal mass ejections.
Artificial Intelligence Based Maintenance Framework for Industrial Machinery
Authors: Al-Fahad Imad Obaid , Hmood Haider S.
Published: February 2022

With the constant evolution of e-manufacturing technologies, there is a clear trend for e-maintenance that involves the integrating of ICT (Information and communication technologies) within the maintenance strategy. This leads to highly sophisticated and complex machinery, which increases the demand for expertise. Unfortunately, a company could always lose the expertise due to experts’ retirement, change of occupation or death. This motivates us in this work to develop an e-maintenance model that enables organizations to exploit expert’s knowledge in the process of machine fault diagnosis. This paper focuses on the building of a Knowledge-Based System (KBS) in order to capture the experts’ knowledge to be permanently kept and cannot be disparaged due to lack of practice. An optimal AI-based tool is proposed that aims at accurate values to retrieve information from KBS, which describes the alarms to diagnose the failure of the machine. An accurate analysis is carried out that yields insight into the impact of KBS on the ability of fault diagnosis. The results illustrate the high-performance of the proposed approach in handling the KBS's data associated.
Motivators of impulsivity to smoke waterpipe tobacco among Nigerian youths: the moderating role of social media normalisation of waterpipe tobacco
Authors: Adu Agatha Oluwafunmilayo , Ismail Nurzali , N Shuhaida Md
Published: February 2022

Background: Impulsivity is a formidable cause of waterpipe tobacco smoking among the youths, however, it is less well studied among African youths. Using PRIME behavioural theory, this study aimed to develop a model that examines the motivators of impulsivity to smoke waterpipe tobacco in linkage to the moderating role of social media normalisation of waterpipe tobacco among youths in Nigeria. Methods: Data were drawn from 695 youths who smoke waterpipe tobacco across six Nigerian universities in the south-west zone using the chain-referral sampling procedure. Descriptive analyses of the obtained data were carried out using the Statistical Package for Social Sciences (SPSS) version 25. The constructs in the developed model were validated through Partial Least Squares Structural Equation Modelling (PLS-SEM) in SmartPLS version 3.Results: Among the Nigerian youths who smoke waterpipe tobacco, intention (β=0.442, P<0.001) is the strongest motivator of impulsivity to smoke waterpipe tobacco as compared to positive evaluations (β=0.302, P<0.001). Additionally, social media normalisation of waterpipe tobacco acts as a moderator that strengthens the relationship between intention and impulsivity (β=0.287, P<0.01) as well as between positive evaluations and impulsivity (β=0.186, P< 0.01) among the youths.Conclusion: Willingness greatly instigates the Nigerian youths’ impulsivity to smoke waterpipe tobacco, and social media normalisation of waterpipe tobacco also considerably increases their impulsivity to smoke waterpipe tobacco. Youth-focused educative waterpipe tobacco cessation-oriented programmes that will utilise diverse constructive-based learning approaches like illustrative learning and counselling, are needed to enlighten and encourage the Nigerian youths on the importance of shunning the desirability to smoke waterpipe tobacco.
Nomogram to Predict Overall and Cancer-Specific Survival in Patients with Primary Bone Diffuse Large B-Cell Lymphoma: A Population-Based Study
Authors: Yang Xing-yao , He Xin , Zhao Yun
Published: February 2022

Background: Primary bone diffuse large B-cell lymphoma accounts for more than 80% of primary bone lymphoma, and as a rare disease, we created two nomogram to predict overall survival (OS) and cancer-specific survival (CSS) in patients with PD-DLBCL.Methods: 891 patients diagnosed with PB-DLBCL between 2007 and 2016 were extracted from the Surveillance, Epidemiology and End Results (SEER) database. Univariate and multivariate Cox analyses were performed to explore independent prognostic factors and to create two separate nomogram for OS and CSS. The area under the curve (AUC), calibration curve, decision curve analysis (DCA), and Kaplan-Meier curve analysis were used to evaluate the nomogram.Results: 4 variables were identified as independent prognostic factors for OS and 3 variables were identified as independent prognostic factors for CSS. the ROC curves demonstrated good discriminatory power of the nomogram. Calibration curves and DCA curves showed that nomogram had satisfactory ability to predict OS and CSS. k-M curves showed that age, gender, first location, chemotherapy, and tumor stage affected patient survival.Conclusions: Age, race, primary site, and chemotherapy are variables that affect OS. And age, race, and chemotherapy are variables that affect CSS in patients with PD-DLBCL. Two nomogram were created based on the above variables to provide more accurate individual survival prediction for PD-DLBCL patients and to help physicians make appropriate clinical decisions.
Clinical Impact of Remnant Lymphatic Invasion on The Recurrence of Esophageal Squamous Cell Carcinoma After Esophagectomy with Neoadjuvant Chemotherapy: A Retrospective Study
Authors: Okuda Sho , Ohuchida Kenoki , Shindo Koji , Moriyama Taiki , Kawata Jun , Tamura Koji , Sada Masafumi , Nagayoshi Kinuko , Mizuuchi Yusuke , Ikenaga Naoki , Nakata Kohei , Oda Yoshinao , Nakamura Masafumi
Published: February 2022

Background: For stage II and III esophageal squamous cell carcinoma (ESCC), neoadjuvant chemotherapy (NAC) followed by esophagectomy is recommended in Japanese guidelines. However, recurrence of ESCC is common regardless of the NAC regimen and operation method, and the effect of NAC on recurrence is limited.Methods: We retrospectively analyzed the outcomes of 54 patients who underwent esophagectomy for ESCC after NAC from 2010 to 2017. We selected 53 patients with ESCC without NAC followed by esophagectomy from 2001 to 2017 for comparison.Results: Among patients who underwent NAC followed by surgery, lymphatic invasion (ly) (hazard ratio: 2.632; 95% CI: 1.04–6.68, p = 0.042) and microscopic residual tumor (R) (hazard ratio: 24.21, 95% CI: 1.92–305, p = 0.014) were independent factors significantly associated with 3-year recurrence-free survival in patients with esophagectomy after NAC in multivariate analysis. In patients without ly after NAC, the presence of lymph node metastasis in pathological diagnosis (pN) was not significantly related to recurrence (p = 0.2401). Recurrence was significantly related to ly (+) (p = 0.0024) in the group with pN after NAC. There were significantly fewer ly (+) patients in the NAC (+) group (p = 0.0332) than those in the NAC (-) group.Conclusion: The presence of ly was an independent risk factor for recurrence of ESCC after esophagectomy following NAC. pN was not a significant recurrence factor for cases with esophagectomy following NAC in multivariate analysis. Adjuvant treatment after surgery may be required in cases with remnant ly after NAC.
Phase 2 Trial of a Vaccine Made from Autologous Dendritic Cells and Lysates from Autologous Tumor-Initiating Cells (AV-GBM-1) in Newly Diagnosed Glioblastoma Patients
Authors: Bota Daniela Annenelie
Published: February 2022

Background
Vaccine immunotherapy may improve survival in glioblastoma (GBM). A multicenter phase II trial was designed to estimate: (1) the success rate of manufacturing the AV-GBM-1 vaccine, (2) adverse events (AE) associated with repeated injections, and (3) survival.
Methods
Fresh tumor tissue was collected during surgery for newly diagnosed GBM. Eligible patients enrolled before starting concurrent radiation therapy and temozolomide (RT/TMZ) with intent-to-treat (ITT) after recovery from RT/TMZ. AV-GBM-1 was made by incubating autologous dendritic cells (DC) with a lysate of irradiated autologous tumor-initiating cells (TIC). Eligible patients were 18–70 years of age, with a Karnofsky Performance Status (KPS) 70 or greater, and which had a successful TIC culture and a sufficient number of monocytes collected. Before subcutaneous (s.c.) injections, a cryopreserved dose was thawed and admixed with 500 mg of granulocyte-macrophage colony-stimulating factor.
Results
Success rates were 97% for both TIC production and monocyte collection. AV-GBM-1 was manufactured for 63/63 patients; 60 enrolled per ITT; 57 started AV-GBM-1. The most common AE attributed to AV-GBM-1 were local injection-site reactions (16%) and flu-like symptoms (10%). Treatment-emergent AE included seizures (33%), headache (37%), and focal neurologic symptoms (28%). One patient discontinued AV-GBM-1 because of seizures. Median progression-free survival (PFS) and overall survival (OS.) from ITT enrollment were 10.3 and 16.0 months, respectively. Median PFS from the first injection was 8.1 months.
Conclusions:
AV-GBM-1 was reliably manufactured. Treatment was well-tolerated, but there were numerous treatment-emergent central nervous system AE, probably primarily due to GBM and its treatment. OS was slightly increased, but median PFS was substantially longer than historical benchmarks.