bluuebunny/crossref_metadata_2025 huggingface dataset:
Recognition of Passive Activity Losses in a Complete Redemption of S Corporation Stock
Authors: Nash Claire Y.
Published: May 2016

Under Section 302(b)(3), a taxpayer who redeems all of the stock he owns in an S corporation is treated as having surrendered his stock in a fully taxable exchange. The taxpayer generally recognizes the gain, or loss, realized on the exchange. Moreover, if the interest he redeems is an interest in a passive activity, Section 469(g) re-characterizes a loss on the exchange, and suspended passive activity losses (PAL) from the activity, as losses not from a passive activity, thus freeing up the suspended losses. However, when the taxpayer and the S corporation are related parties, Section 469(g)(1)(B) prohibits recognition of suspended PAL until the S corporation disposes of the “interest acquired” in a taxable transaction. Following a redemption, disposition of the “interest acquired” is not literally possible. Consequently, taxpayers question whether related-party S corporation shareholders lose the ability to recognize suspended PAL when they redeem their stock. For several years, the American Institute of Certified Public Accountants (AICPA) Tax Division has requested guidance from the Treasury Department regarding this question and listed this issue as the top priority item affecting S corporation taxation. The AICPA posits that following a 302(b)(3) redemption, suspended PAL of related-party shareholders disappear. Is the AICPA's literal application of the statute the correct approach? The legal analysis herein answers the question raised by the AICPA and taxpayers and provides guidance to Treasury in drafting administrative authority that is consistent with existing statutes and the legislative history of Section 469. Although Section 469(g)(1)(B) is problematic for related-party S corporation shareholders, their suspended PAL do not disappear. Section 469(g)(1)(B) postpones the recognition of suspended PAL in accordance with the aggregate approach when there is a complete redemption of a related-party S corporation shareholder's interest under Section 302(b)(3).
Neck Posture Clusters and Their Association With Biopsychosocial Factors and Neck Pain in Australian Adolescents
Authors: Richards Karen V. , Beales Darren J. , Smith Anne J. , O'Sullivan Peter B. , Straker Leon M.
Published: May 2016

Background

There is conflicting evidence on the association between sagittal neck posture and neck pain.

Objective

The purposes of this study were: (1) to determine the existence of clusters of neck posture in a cohort of 17-year-olds and (2) to establish whether identified subgroups were associated with biopsychosocial factors and neck pain.

Design

This was a cross-sectional study.

Methods

The adolescents (N=1,108) underwent 2-dimensional photographic postural assessment in a sitting position. One distance and 4 angular measurements of the head, neck, and thorax were calculated from photo-reflective markers placed on bony landmarks. Subgroups of sagittal sitting neck posture were determined by cluster analysis. Height and weight were measured, and lifestyle and psychological factors, neck pain, and headache were assessed by questionnaire. The associations among posture subgroups, neck pain, and other factors were evaluated using logistic regression.

Results

Four distinct clusters of sitting neck posture were identified: upright, intermediate, slumped thorax/forward head, and erect thorax/forward head. Significant associations between cluster and sex, weight, and height were found. Participants classified as having slumped thorax/forward head posture were at higher odds of mild, moderate, or severe depression. Participants classified as having upright posture exercised more frequently. There was no significant difference in the odds of neck pain or headache across the clusters.

Limitations

The results are specific to 17-year-olds and may not be applicable to adults.

Conclusion

Meaningful sagittal sitting neck posture clusters were identified in 17-year-olds who demonstrated some differences with biopsychosocial profiling. The finding of no association between cluster membership and neck pain and headaches challenges widely held beliefs about the role of posture in adolescent neck pain.
Evaluation of the enterovirus laboratory surveillance system in Denmark, 2010 to 2013
Authors: Condell Orla , Midgley Sofie , Christiansen Claus Bohn , Chen Ming , Chen Nielsen Xiaohui , Ellermann-Eriksen Svend , Mølvadgaard Mette , Schønning Kristian , Vermedal Hoegh Silje , Andersen Peter Henrik , Voldstedlund Marianne , Fischer Thea Kølsen
Published: May 2016

The primary aim of the Danish enterovirus (EV) surveillance system is to document absence of poliovirus infection. The conflict in Syria has left many children unvaccinated and movement from areas with polio cases to Europe calls for increased awareness to detect and respond to virus-transmission in a timely manner. We evaluate the national EV laboratory surveillance, to generate recommendations for system strengthening. The system was analysed for completeness of viral typing analysis and clinical information and timeliness of specimen collection, laboratory results and reporting of clinical information. Of 23,720 specimens screened, 2,202 (9.3%) were EV-positive. Submission of cerebrospinal fluid and faecal specimens from primary diagnostic laboratories was 79.5% complete (845/1,063), and varied by laboratory and patient age. EV genotypes were determined in 68.5% (979/1,430) of laboratory-confirmed cases, clinical information was available for 63.1% (903/1,430). Primary diagnostic results were available after a median of 1.4 days, typing results after 17 days, detailed clinical information after 33 days. The large number of samples typed demonstrated continued monitoring of EV-circulation in Denmark. The system could be strengthened by increasing the collection of supplementary faecal specimens, improving communication with primary diagnostic laboratories, adapting the laboratory typing methodology and collecting clinical information with electronic forms.
We Come to Form Ourselves Bit by Bit
Authors: Rubin Beth C.
Published: May 2016

Over the past several decades, the implementation of democratic citizenship education has become a common prescription for the civic reconstruction of post-conflict societies. Across the globe, educational changes are seen as fundamental to the creation of peaceful, tolerant, and democratic civic identities, the key to “social reconstruction, a better future, and a lasting peace.” Drawing on qualitative data from varied schools in postwar Guatemala, this article illustrates a critical dilemma in post-conflict civic education: the difficulties of engaging directly with past and present injustice while moving toward a shared national identity. Global models of democratic, multicultural, and human rights education alone are inadequate for creating a new sense of citizenship in a country in which young people’s sense of belonging and their interpretations of the past are deeply connected to how their communities are positioned within a profoundly inequitable power structure.
Fuzzy Clustering Based Blind Adaptive OFDM System
Authors: Twayana Krishna Sundar , Joshi Shashidhar Ram
Published: May 2016

This paper present blind adaptive modulation for OFDM system. Automatic recognition of the modulation level of detected signal and blind estimation of SNR is a major task of an intelligent receiver presented here. In this paper, modulation classification is performed by fuzzy c-means clustering followed by new approach of modulation classification, fuzzy template matching considering the constellation of the received signal. In addition, SNR estimation and modulation order for the next transmission is carry out by Fuzzy Logic Interface based upon heuristics. The simulation that has been conducted shows high capability of recognition of modulation level, almost perfect estimation of SNR and adaptive with considerable BER in an Additive White Gaussian Noise channel. The performance of proposed system is simulated in computer simulation tool, MATLAB 7.0.

Journal of Advanced College of Engineering and Management, Vol.1, 2015, pp. 51-58
Comparison of surgical and visual outcomes following capsulotomy using the envelope and continuous curvilinear capsulorhexis technique in manual small incision cataract surgery
Authors: Chaudhary Sanjib Kr , Pokhrel Shailesh Mani , Sharma Manoj , Badhu Badri Prasad , Shrestha Bhuwan Govinda , Chaudhary Santosh
Published: May 2016

Background & Objectives: Capsulotomy is one of the important step in cataract surgery which can affect the surgical outcomes. The aim of the study was to compare the outcomes of envelope and continuous curvilinear capsulorhexis technique in manual small incision cataract surgery (MSICS). Materials & Methods: A total of 72 eyes of 72 patients undergoing MSICS were studied. The patients were equally divided into two groups i.e. 36 patients each in envelope capsulotomy (Group A) and continuous curvilinear capsulotomy (CCC) group (Group B).Results: Mean surgical time (±SD) in Group A was 355.83 sec ±37.79 sec and in Group B was 375 sec ±31.214 (p=0.02). Uncorrected visual acuity on postoperative day 1 was 6/9 or better in 50% (18) cases in Group A and in 36% (13) cases in Group B (p=0.28). Best corrected visual acuity (BCVA) on post-operative week 12 was 6/9 or better in 30.6% (n=11) cases in Group A and in 36% (n=13) cases in Group B (p=0.43). On 12th week postoperatively, Grade 2 posterior capsular opacification (PCO) was seen in 30.6 % (n=11) cases of Group A and in 16.6 % (n=6) cases of Group B (p=0.17). Conclusion: CCC technique can be considered superior to envelope technique for long term visual rehabilitation.JCMS Nepal. 2016;12(1):10-13.
A study of anemia among adolescent girls in eastern part of Nepal
Authors: Kanodia Piush , Bhatta Mukesh , Singh Rupa Rajbhandari , Bhatta Nisha K , Shah Gauri Shankar
Published: May 2016

Background & Objectives: Anemia is a global health problem. About 40% of the world's population suffers from anemia and adolescence is one of the most vulnerable age group. Hence the objective of the study was to determine prevalence and distribution of anemia among adolescent girls in eastern part of Nepal.Materials & Methods: This cross sectional study was conducted in a Government School of Dharan over a period of one year. There were total 433 participants, whose clinical and demographic profile were recorded and analyzed. Hemoglobin estimation was done by using cyanmethaemoglobin method and anemia was defined as per WHO cut-off.Results: The overall prevalence of anemia was found to be 51.3%. Prevalence was significantly more in pre-menarche age and undernourished girls (p<0.05). However factors like diet (vegetarian/non-vegetarian), worm infestation and parental education did not have a significant impact on occurrence of anemia.Conclusion: Anemia is the major health problem among adolescent girls in eastern part Nepal with high prevalence rate and nutrition is one of the leading causative factors for anemia.JCMS Nepal. 2016;12(1):19-22.
Evaluation of chemokine receptors (CCRs) expression on peripheral blood T-lymphocyte subsets in patients with thoracic aortic aneurysm
Authors: Tiwari Kaushal Kishore , Sbrana Silverio , Bevilacqau Stefano , Gasbari Tommaso , Santarelli Filippo , Solinas Marco , Glauber Mattia
Published: May 2016

Background & Objectives: Mortality and morbidity from the complication of aortic aneurysm remain very high. Aortic size index, which classify thoracic aortic aneurysm patients in three risk groups for aortic rupture prediction. Recent data support that aortic wall remodeling is a dynamic process with active involvement of the chronic inflammation and immunological system. Aim of our study is to evaluate expression level of chemokine receptors known to be involved in the T-cells migration and to correlate them with aortic size index. Materials & Methods: Total 20 patients undergoing surgery for ascending aortic aneurysm and/or aortic valve surgery were enrolled. Aortic size index was calculated. Preoperatively blood samples collected. By flowcytometry and dual parameter dot plot technology percentage of positivity of CCR5 on these T-cell subsets were quantified. Results: Mean age of the patients was 67±5.93 years. Majority of patients had hypertension. Mean ascending aortic diameter was 42.1±8.14 mm. Mean Aortic size Index was 22.21±3.38 mm/m2. A statistical significance has observed between aortic size index and the expression of CCR5 on total CD4 positive T-cells (p-0.0949), and between aortic size index and CCR5 expression on the total CD3 positive T-cells (p-0.0293). Significant correlation observed between ASI and CCR5 expression on the CD8+/CD3+ T-cell subset (p-0.0183). Similarly, strong positive relationship between ASI and the expression of CCR5 on the cytotoxic CD28-/CD4+ T-cell subset (p-0.0055). Activated state of cytotoxic CD28-/CD4+ cell also correlated with aortic size index (p-0.0668).Conclusion: We conclude that T-cell mediated cytotoxic mechanism driven by CCR5 play an important role in the pathophysiology of the thoracic aortic aneurysm.JCMS Nepal. 2016;12(1):23-27.
Quality Assessment of Some Rice Varieties Newly Adopted in NARC
Authors: Subedi Ujjwol , Mishra Achyut , Shrestha Man Bahadur
Published: May 2016

Five rice varieties newly adopted in NARC were studied for their varietal differences in relation to quality characteristics such as physicochemical and cooking qualities. In physical analysis bulk density, density, thousand kernel weight and length to breadth ratio were recorded as highest in ‘Lumle-2’ (0.602gm/ml), ‘Lumle-2’ (27.789gm), ‘UPLRI-5’ (1.361gm/ml) and ‘IET-16775’ (4.710) varieties respectively. The milling and head rice recovery were recorded maximum in ‘Lumle-2’ (78.821%) and ‘UPLRI-5’ (78.282%) respectively. Protein, fat, amylose, crude-fiber and total-ash were ranged from 8.292-13.346%, 1.147-1.872%, 24.541-27.396% and 0.707-1.379% respectively. Iron, phosphorous and calcium content were estimated in the range 1.016-2.325mg/100g, 204.036-336.532mg/100g and 16.189-29.813mg/100g respectively. From organoleptic test, ‘IET-16775’ variety was scored as strongest aroma. ‘Lumle-2’, ‘Chhomrong’, ‘Machapuchhre-3’ varieties were shown as intermediate gelatinization-temperature and ‘UPLRI-5’ and ‘IET-16775’ varieties were recorded as high gelatinization-temperature. Water uptake ratio, cooking time, elongation ratio and gruel loss were found in the range 1.696-2.667, 18-25minutes, 1.252-1.428, and 2.477-6.188% respectively.
Quality Evaluation and Storage Study of Chicken Meat Pickle
Authors: Rajbanshi Sunil , Adhikari Bhaskar Mani , Subba Dilip
Published: May 2016

A study was undertaken to develop shelf stable intermediate moisture type chicken meat pickle and evaluate its quality and storage stability. Pre-cooked and fried lean minced chicken meat was mixed with salt, spices and vinegar and packed air tight in glass jar. The product had appreciable sensory quality and low total microbial, yeast and mould counts. Salmonella, Staphylococci and E. coli were found absent in the product. Microbial count and peroxide value lay within the acceptable levels for sixty days.
Comparative Evaluation of the Levels of Minerals and Mineral Safety Index of Raw, Roasted and Cooked Treculia Africana Seed Flours
Authors: Adesina Adeolu Jonathan , Adeyeye Emmanuel Ilesanmi
Published: May 2016

The raw, cooked and roasted seed parts of Treculia africana commonly called “afon” in Yoruba and “ukwa” in Ibo languages were investigated for the mineral composition using standard methods. Calculations of Ca/P, Na/K, [K/(Ca+Mg)], Ca/Mg ratios and mineral safety index were also carried out. In the mineral composition, raw samples were best in Na and K; roasted samples were highest in Ca and Mg; cooked samples  were highest in Zn, Fe,  Mn and P; in all the samples, Ca/P was generally lower than the 0.5 recommended for favourable absorption of calcium in the intestine  for bone formation;  the samples were all good in Na/K in that they were all lower than 0.6 recommended as the  highest limit ratio that prevent high blood pressure; both the raw and processed samples were high in [K/(Ca+Mg)] and non of the sample type was good in Ca/Mg. In the mineral safety index (MSI) values:  Fe with  standard MSI of  6.7 recorded negative values of -3.20 and -4.20 in roasted seeds testa and cooked testa flours respectively whereas in all other sample parts positive values were recorded. The statistical analysis results (linear correlation) on a pair-wise comparison showed that the mineral contents in raw/roasted and raw/cooked were significantly different at ? = 0.05, df=n-1. The research has provided nutritional information with respect to processing methods on the minerals in the various parts of the seeds analyzed and that levels of nutritive minerals were more positively enhanced in the roasted seeds flour than in the cooked sample flours.
Clear Lens Extraction with Foldable Intraocular Lens Implantation in a Case of Isolated Microspherophakia
Authors: Shrestha Chunu , Pandey Manish
Published: May 2016

Microspherophakia is an uncommon bilateral eye condition in which there is abnormal laxity of the lens zonules, leading to development of a spherical lens. Microspherophakia without systemic features is a rare presentation. The management of such a case is quite challenging. We report a case of isolated microspherephakia which was managed successfully with clear lens extraction with implantation of a foldable intraocular lens.
Comparison of Coronally Advanced Flap with Semilunar Coronally Repositioned Flap with Sutures for the Treatment of Gingival Recession
Authors: Rana Sweta Rajya Laxmi , Shrestha Surendra Man , Pradhan Ameena
Published: May 2016

Introduction: The surgical correction of shallow gingival recession by coronally advanced flap (CAF) and its variant semilunar coronally repositioned flap (SLF) offers the advantage of a single surgical site, less patient discomfort and predictable root coverage with good colour match. This study aims to compare and evaluate the predictability and reliability of the two types of flaps.Methods: In 20 Miller’s Class I recession defects, 10 sites each were treated with either SLF or CAF. Recession height, recession width, clinical attachment loss, width of keratinized gingiva, Plaque Index and Gingival Index were assessed at baseline, 1, 3 and 6 months. The intra-group comparison was done using paired t-test and the inter-group comparison using independent t-test with statistical p value set at <0.05.Results: Average root coverage seen with Group I (SLF) at 1 month was 51.67% and with Group II (CAF) was 62.5% and at 6 months the root coverage was 42.5% for both the groups. In both Groups, statistically significant changes in recession width and recession height were seen from baseline to 6 months. No statistically significant difference was seen between the two groups at any time period.Conclusions: The coronally advanced flap is predictable in the treatment of gingival recessions. However the semilunar flap with additional stabilizing suture may also be beneficial in treating gingival recessions and further research is needed in this technique.
Amiri Baraka: A Lifetime of Saying the Unsayable
Authors: Keleta-Mae Naila
Published: May 2016

Through the analysis of three poems written at distinct moments in his prolific fifty-year career, this article argues that a central legacy of the work of Amiri Baraka (LeRoi Jones) is the creation of art and scholarship that publicly critiques and provokes the status quo—time and time again, Baraka said the unsayable.
Foramen magnum meningiomas: surgical results and risks predicting poor outcomes based on a modified classification
Authors: Li Da , Wu Zhen , Ren Cong , Hao Shu-Yu , Wang Liang , Xiao Xin-Ru , Tang Jie , Wang Yong-Gang , Meng Guo-Lu , Zhang Li-Wei , Zhang Jun-Ting
Published: May 2016

OBJECTIVE

This study aimed to evaluate neurological function and progression/recurrence (P/R) outcome of foramen magnum meningioma (FMM) based on a modified classification.

METHODS

This study included 185 consecutive patients harboring FMMs (mean age 49.4 years; 124 females). The authors classified the FMMs into 4 types according to the previous classification of Bruneau and George as follows: Type A (n = 49, 26.5%), the dural attachment of the lesion grows below the vertebral artery (VA); Type B (n = 39, 21.1%), the dural attachment of the lesion grows above the VA; Type C1 (n = 84, 45.4%), the VA courses across the lesion with or without VA encasement or large lesions grow both above and below the bilateral VA; and Type C2 (n = 13, 7.0%), Type C1 plus partial/total encasement of the VA and extradural growth.

RESULTS

The median preoperative Karnofsky Performance Scale (KPS) score was 80. Gross-total resection (GTR) was achieved in 154 patients (83.2%). Lower cranial nerve morbidity was lowest in Type A lesions (16.3%). Type C2 lesions were inherently larger (p = 0.001), had a greater percentage of ventrolateral location (p = 0.009) and VA encasement (p < 0.001), lower GTR rate (p < 0.001), longer surgical duration (p = 0.015), higher morbidity (38.5%), higher P/R rate (30.8%, p = 0.009), and poorer recent KPS score compared with other types. After a mean follow-up duration of 110.3 months, the most recent follow-up data were obtained in 163 patients (88.1%). P/R was observed in 13 patients (7.2%). The median follow-up KPS score was 90. Compared with preoperative status, recent neurological status was improved in 91 (49.2%), stabilized in 76 (41.1%), and worsened in 18 (9.7%) patients. The multivariate Cox proportional hazard regression model demonstrated Type C2 (HR 3.94, 95% CI 1.04–15.0, p = 0.044), nontotal resection (HR 6.30, 95% CI 1.91–20.8, p = 0.003), and pathological mitosis (HR 7.11, 95% CI 1.96–25.8, p = 0.003) as independent adverse predictors for tumor P/R. Multivariate logistic regression analysis identified nontotal resection (OR 4.06, 95% CI 1.16–14.2, p = 0.029) and pathological mitosis (OR 6.29, 95% CI 1.47–27.0, p = 0.013) as independent risks for poor outcome (KPS score < 80).

CONCLUSIONS

The modified classification helped to predict surgical outcome and P/R in addition to the position of the lower cranial nerves. Preoperative imaging studies and neurological function should be reviewed carefully to establish an individualized management strategy to improve long-term outcome.
Multifocal granulocytic sarcoma of the spine mimicking neurofibromatosis Type 2: case report
Authors: Lekovic Gregory P. , Schwartz Marc S. , Go John L.
Published: May 2016

In this report the authors report on a patient with a very indolent course of granulocytic sarcoma, characterized by steroid-induced remission of spinal and cranial tumors and recurrence over a period of several years. This 24-year-old man with history of leukemia presented with rapid-onset quadriparesis secondary to multiple extraaxial masses of the cervicothoracic spine, from C-5 to T-3, and lumbosacral spine, from L-5 to the coccyx. Although the imaging features were highly suggestive of neurofibromatosis Type 2, the patient's history and clinical course were consistent with granulocytic sarcoma; repeat imaging and, later, needle biopsy definitively established the diagnosis of granulocytic sarcoma. Laminectomy and surgical decompression of the spine were not required and, arguably, could have posed unnecessary risk to the patient. This case illustrates that the successful management of a patient presenting with profound neurological deficits due to intradural spinal cord tumors may sometimes be nonsurgical.
The American Cancer Society challenge goal to reduce US cancer mortality by 50% between 1990 and 2015: Results and reflections
Authors: Byers Tim , Wender Richard C. , Jemal Ahmedin , Baskies Arnold M. , Ward Elizabeth E. , Brawley Otis W.
Published: May 2016

In 1996, the Board of Directors of the American Cancer Society (ACS) challenged the United States to reduce what looked to be possible peak cancer mortality in 1990 by 50% by the year 2015. This analysis examines the trends in cancer mortality across this 25‐year challenge period from 1990 to 2015. In 2015, cancer death rates were 26% lower than in 1990 (32% lower among men and 22% lower among women). The 50% reduction goal was more fully met for the cancer sites for which there was enactment of effective approaches for prevention, early detection, and/or treatment. Among men, mortality rates dropped for lung cancer by 45%, for colorectal cancer by 47%, and for prostate cancer by 53%. Among women, mortality rates dropped for lung cancer by 8%, for colorectal cancer by 44%, and for breast cancer by 39%. Declines in the death rates of all other cancer sites were substantially smaller (13% among men and 17% among women). The major factors that accounted for these favorable trends were progress in tobacco control and improvements in early detection and treatment. As we embark on new national cancer goals, this recent past experience should teach us that curing the cancer problem will require 2 sets of actions: making new discoveries in cancer therapeutics and more completely applying those discoveries in cancer prevention we have already made. CA Cancer J Clin 2016;66:359–369. © 2016 American Cancer Society.
The Diversity of Ribonuclease P: Protein and RNA Catalysts with Analogous Biological Functions
Authors: Klemm Bradley , Wu Nancy , Chen Yu , Liu Xin , Kaitany Kipchumba , Howard Michael , Fierke Carol
Published: May 2016

Ribonuclease P (RNase P) is an essential endonuclease responsible for catalyzing 5’ end maturation in precursor transfer RNAs. Since its discovery in the 1970s, RNase P enzymes have been identified and studied throughout the three domains of life. Interestingly, RNase P is either RNA-based, with a catalytic RNA subunit, or a protein-only (PRORP) enzyme with differential evolutionary distribution. The available structural data, including the active site data, provides insight into catalysis and substrate recognition. The hydrolytic and kinetic mechanisms of the two forms of RNase P enzymes are similar, yet features unique to the RNA-based and PRORP enzymes are consistent with different evolutionary origins. The various RNase P enzymes, in addition to their primary role in tRNA 5’ maturation, catalyze cleavage of a variety of alternative substrates, indicating a diversification of RNase P function in vivo. The review concludes with a discussion of recent advances and interesting research directions in the field.
Advances in Autophagy Regulatory Mechanisms
Authors: Gallagher Laura , Williamson Leon , Chan Edmond
Published: May 2016

Autophagy plays a critical role in cell metabolism by degrading and recycling internal components when challenged with limited nutrients. This fundamental and conserved mechanism is based on a membrane trafficking pathway in which nascent autophagosomes engulf cytoplasmic cargo to form vesicles that transport their content to the lysosome for degradation. Based on this simple scheme, autophagy modulates cellular metabolism and cytoplasmic quality control to influence an unexpectedly wide range of normal mammalian physiology and pathophysiology. In this review, we summarise recent advancements in three broad areas of autophagy regulation. We discuss current models on how autophagosomes are initiated from endogenous membranes. We detail how the uncoordinated 51-like kinase (ULK) complex becomes activated downstream of mechanistic target of rapamycin complex 1 (MTORC1). Finally, we summarise the upstream signalling mechanisms that can sense amino acid availability leading to activation of MTORC1.
Entropy-Based Incomplete Cholesky Decomposition for a Scalable Spectral Clustering Algorithm: Computational Studies and Sensitivity Analysis
Authors: Langone Rocco , Van Barel Marc , Suykens Johan
Published: May 2016

Spectral clustering methods allow datasets to be partitioned into clusters by mapping the input datapoints into the space spanned by the eigenvectors of the Laplacian matrix. In this article, we make use of the incomplete Cholesky decomposition (ICD) to construct an approximation of the graph Laplacian and reduce the size of the related eigenvalue problem from N to m, with m ≪ N . In particular, we introduce a new stopping criterion based on normalized mutual information between consecutive partitions, which terminates the ICD when the change in the cluster assignments is below a given threshold. Compared with existing ICD-based spectral clustering approaches, the proposed method allows the reduction of the number m of selected pivots (i.e., to obtain a sparser model) and at the same time, to maintain high clustering quality. The method scales linearly with respect to the number of input datapoints N and has low memory requirements, because only matrices of size N × m and m × m are calculated (in contrast to standard spectral clustering, where the construction of the full N × N similarity matrix is needed). Furthermore, we show that the number of clusters can be reliably selected based on the gap heuristics computed using just a small matrix R of size m × m instead of the entire graph Laplacian. The effectiveness of the proposed algorithm is tested on several datasets.
Information Is Not a Virus, and Other Consequences of Human Cognitive Limits
Authors: Lerman Kristina
Published: May 2016

The many decisions that people make about what to pay attention to online shape the spread of information in online social networks. Due to the constraints of available time and cognitive resources, the ease of discovery strongly impacts how people allocate their attention to social media content. As a consequence, the position of information in an individual’s social feed, as well as explicit social signals about its popularity, determine whether it will be seen, and the likelihood that it will be shared with followers. Accounting for these cognitive limits simplifies mechanics of information diffusion in online social networks and explains puzzling empirical observations: (i) information generally fails to spread in social media and (ii) highly connected people are less likely to re-share information. Studies of information diffusion on different social media platforms reviewed here suggest that the interplay between human cognitive limits and network structure differentiates the spread of information from other social contagions, such as the spread of a virus through a population.
Opportunities for Environmental Noise Mapping in Saudi Arabia: A Case of Traffic Noise Annoyance in an Urban Area in Jeddah City
Authors: Zytoon Mohamed
Published: May 2016

As the traffic and other environmental noise generating activities are growing in The Kingdom of Saudi Arabia (KSA), adverse health and other impacts are expected to develop. The management of such problem involves many actions, of which noise mapping has been proven to be a helpful approach. The objective of the current study was to test the adequacy of the available data in KSA municipalities for generating urban noise maps and to verify the applicability of available environmental noise mapping and noise annoyance models for KSA. Therefore, noise maps were produced for Al-Fayha District in Jeddah City, KSA using commercially available noise mapping software and applying the French national computation method “NMPB” for traffic noise. Most of the data required for traffic noise prediction and annoyance analysis were available, either in the Municipality GIS department or in other governmental authorities. The predicted noise levels during the three time periods, i.e., daytime, evening, and nighttime, were found higher than the maximum recommended levels established in KSA environmental noise standards. Annoyance analysis revealed that high percentages of the District inhabitants were highly annoyed, depending on the type of planning zone and period of interest. These results reflect the urgent need to consider environmental noise reduction in KSA national plans. The accuracy of the predicted noise levels and the availability of most of the necessary data should encourage further studies on the use of noise mapping as part of noise reduction plans.
Brazilian Green Propolis Improves Antioxidant Function in Patients with Type 2 Diabetes Mellitus
Authors: Zhao Liting , Pu Lingling , Wei Jingyu , Li Jinghua , Wu Jianquan , Xin Zhonghao , Gao Weina , Guo Changjiang
Published: May 2016

Propolis contains a variety of bioactive components and possesses many biological properties. This study was designed to evaluate potential effects of Brazilian green propolis on glucose metabolism and antioxidant function in patients with type 2 diabetes mellitus (T2DM). In the 18-week randomized controlled study, enrolled patients with T2DM were randomly assigned to Brazilian green propolis group (900 mg/day) (n = 32) and control group (n = 33). At the end of the study, no significant difference was found in serum glucose, glycosylated hemoglobin, insulin, aldose reductase or adiponectin between the two groups. However, serum GSH and total polyphenols were significantly increased, and serum carbonyls and lactate dehydrogenase activity were significantly reduced in the Brazilian green propolis group. Serum TNF-α was significantly decreased, whereas serum IL-1β and IL-6 were significantly increased in the Brazilian green propolis group. It is concluded that Brazilian green propolis is effective in improving antioxidant function in T2DM patients.
A Semantic Registry Method Using Sensor Metadata Ontology to Manage Heterogeneous Sensor Information in the Geospatial Sensor Web
Authors: Xiao Changjiang , Chen Nengcheng , Wang Xiaolei , Chen Zeqiang
Published: May 2016

Efficient information management and precise discovery of heterogeneous sensors in the Geospatial Sensor Web (GSW) are a major challenge. Intelligent sensor management requires a registry service to store and process sensor information efficiently. In this paper, we propose a Sensor Metadata Ontology (SMO) to achieve a unified semantic description for heterogeneous sensors that is used to express sensor semantics. Through mapping between the sensor registry information model and the SMO, the sensor metadata could be stored with semantic information for the registry. The framework of a Sensor Semantic Registry Service (SSRS) has been successfully implemented for the registration and discovery of heterogeneous sensors. The results of GEOSENSOR-SSRS experiments show that the proposed semantic registry method can be used to enable sharing in an open distributed sensor network as well as to promote accuracy and efficiency of discovery.
The Effect of Minimally Invasive Hematoma Aspiration on the JNK Signal Transduction Pathway after Experimental Intracerebral Hemorrhage in Rats
Authors: Pei Haitao , Jiang Tao , Liu Guofang , Li Zhaoxing , Luo Kai , An Jingjiao , Li Guangcheng , Guo Yunliang
Published: May 2016

Objective: To explore the effect of minimally invasive hematoma aspiration (MIHA) on the c-Jun NH2-terminal kinase (JNK) signal transduction pathway after intracerebral hemorrhage (ICH). Methods: In this experiment, 300 adult male Wistar rats were randomly and averagely divided into sham-operated group, ICH group and MIHA group. In each group, 60 rats were used in the detection of indexes in this experiment, while the other 40 rats were used to replace rats which reached the exclusion criteria (accidental death or operation failure). In ICH group and MIHA group, ICH was induced by injection of 70 µL of autologous arterial blood into rat brain, while only the rats in MIHA group were treated by MIHA 6 h after ICH. Rats in sham-operated group were injected nothing into brains, and they were not treated either, like rats in ICH group. In each group, six rats were randomly selected to observe their Bederson’s scales persistently (6, 24, 48, 72, 96, 120 h after ICH). According to the time they were sacrificed, the remaining rats in each group were divided into 3 subgroups (24, 72, 120 h). The change of brain water content (BWC) was measured by the wet weight to dry weight ratio method. The morphology of neurons in cortex was observed by the hematoxylin–eosin (HE) staining. The expressions of phospho-c-Jun NH2-terminal kinase (pJNK) and JNK in peri-hematomal brain tissue were determined by the immunohistochemistry (IHC) and Western blotting (WB). Results: At all time points, compared with the ICH groups, the expression of pJNK decreased obviously in MIHA groups (p < 0.05), while their Bederson’s scales and BWC declined, and neuron injury in the cortex was relieved. The expression level of JNK was not altered at different groups. The data obtained by IHC and WB indicated a high-level of consistency, which provided a certain dependability of the test results. Conclusion: The JNK signal transduction pathway could be activated after intracerebral hemorrhage, with the expressions of pJNK increasing. MIHA could relieve the histo-pathological damage of nerve cells, reducing brain edema and neurological deficits, and these neuroprotective effects might be associated with suppression of JNK signal transduction pathway.
The Relationship between NALP3 and Autoinflammatory Syndromes
Authors: Campbell Lorna , Raheem Irfan , Malemud Charles , Askari Ali
Published: May 2016

The nucleotide-binding domain, leucine-rich repeat/pyrin domain-containing-3 (NALP3) inflammasome, which is required for synthesis of interleukin-1β, has been implicated in the pathogenesis of several autoinflammatory syndromes. This review of the literature summarizes the interconnectedness of NALP3 inflammasome with some of these disorders. Familial Mediterranean fever results from a mutation in the Mediterranean fever (MEFV) gene, which encodes the pyrin protein. Previous study results suggest that pyrin suppresses caspase-1 activation, perhaps by competing for the adaptor protein, termed, pyrin domain of apoptosis/speck-like protein containing a caspase-recruitment domain (ACS) which therefore interferes with NALP3 inflammasome activation. The nucleotide-binding domain, leucine-rich repeat/pyrin domain-containing-3 (NALP3) inflammasome is constitutively activated in cryopyrin-associated periodic syndromes due to gain-of-function mutations resulting from point mutations within the neuronal apoptosis inhibitor protein/class 2 transcription factor/heterokaryon incompatibility/telomerase-associated protein-1 (NACHT) domain of the NALP3 protein. Pyogenic arthritis, pyoderma gangrenosum and acne (PAPA) syndrome is caused by mutations in the genes encoding proline-serine-threonine phosphatase interacting protein 1 (PSTPIP1). These PSTPIP1 mutants are thought to bind to pyrin causing an increase in the pyrin domain of apoptosis/speck-like protein containing a caspase-recruitment domain (ASC) pyroptosome assembly leading to procaspase-1 recruitment and therefore its activation. Hyperimmunoglublinemia D syndrome is caused by mevalonate kinase (MVK) deficiency, which may be affected by protein accumulation that leads to NALP3 inflammasome activation. Tumor necrosis factor receptor–associated periodic syndrome is associated with mutations in the tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A) gene which decreases the level of soluble tumor necrosis factor receptor-1 (TNFR1) leading to neutralization of tumor necrosis factor (TNF)-α. In general, these autoinflammatory disorders have shown a clinical response to interleukin-1 (IL-1) antagonists, suggesting that the NALP3 inflammasome serves a critical role in their pathogenesis.
Hybrid Cells Derived from Human Breast Cancer Cells and Human Breast Epithelial Cells Exhibit Differential TLR4 and TLR9 Signaling
Authors: Tosun Songül , Fried Sabrina , Niggemann Bernd , Zänker Kurt , Dittmar Thomas
Published: May 2016

TLRs are important receptors of cells of the innate immune system since they recognize various structurally conserved molecular patterns of different pathogens as well as endogenous ligands. In cancer, the role of TLRs is still controversial due to findings that both regression and progression of tumors could depend on TLR signaling. In the present study, M13SV1-EGFP-Neo human breast epithelial cells, MDA-MB-435-Hyg human breast cancer cells and two hybrids M13MDA435-1 and -3 were investigated for TLR4 and TLR9 expression and signaling. RT-PCR data revealed that LPS and CpG-ODN induced the expression of pro-inflammatory cytokines, like IFN-β, TNF-α, IL-1β and IL-6 in hybrid cells, but not parental cells. Interestingly, validation of RT-PCR data by Western blot showed detectable protein levels solely after LPS stimulation, suggesting that regulatory mechanisms are also controlled by TLR signaling. Analysis of pAKT and pERK1/2 levels upon LPS and CpG-ODN stimulation revealed a differential phosphorylation pattern in all cells. Finally, the migratory behavior of the cells was investigated showing that both LPS and CpG-ODN potently blocked the locomotory activity of the hybrid cells in a dose-dependent manner. In summary, hybrid cells exhibit differential TLR4 and TLR9 signaling.
The Use of 1α,25-Dihydroxyvitamin D3 as an Anticancer Agent
Authors: Marcinkowska Ewa , Wallace Graham , Brown Geoffrey
Published: May 2016

The notion that vitamin D can influence the incidence of cancer arose from epidemiological studies. The major source of vitamin D in the organism is skin production upon exposure to ultra violet-B. The very first observation of an inverse correlation between exposure of individuals to the sun and the likelihood of cancer was reported as early as 1941. In 1980, Garland and Garland hypothesised, from findings from epidemiological studies of patients in the US with colon cancer, that vitamin D produced in response to sun exposure is protective against cancer as opposed to sunlight per se. Later studies revealed inverse correlations between sun exposure and the occurrence of prostate and breast cancers. These observations prompted laboratory investigation of whether or not vitamin D had an effect on cancer cells. Vitamin D is not active against cancer cells, but the most active metabolite 1α,25-dihydroxyvitamin D3 (1,25D) has profound biological effects. Here, we review the anticancer action of 1,25D, clinical trials of 1,25D to date and the prospects of the future therapeutic use of new and low calcaemic analogues.
One-Step Method to Prepare PLLA Porous Microspheres in a High-Voltage Electrostatic Anti-Solvent Process
Authors: Wang Ying , Zhu Li-Hui , Chen Ai-Zheng , Xu Qiao , Hong Yu-Juan , Wang Shi-Bin
Published: May 2016

A one-step method using a high-voltage electrostatic anti-solvent process was employed to fabricate poly-l-lactide (PLLA) porous microspheres (PMs). To address the simplification and control of the preparation process, a 24 full factorial experiment was performed to optimize the operating process and analyze the effect of the factors on the morphology and aerodynamic properties of the PLLA PMs, and various characterization tests were also performed. The resulting PLLA PMs exhibited an even and porous morphology with a density less than 0.4 g/cm3, a geometric mean diameter (Dg) of 10–30 μm, an aerodynamic diameter (Da) of 1–5 μm, a fine particle fraction (FPF) of 56.3%, and a porosity of 76.2%, meeting the requirements for pulmonary drug delivery. The physicochemical characterizations reveal that no significant chemical change occurred in the PLLA during the process. An investigation of its in vitro cytotoxicity and pulmonary toxicity shows no obvious toxic response, indicating good biosafety. This study indicates that the one-step method using a high-voltage electrostatic anti-solvent process has great potential in developing an inhalable drug carrier for pulmonary drug delivery.
Antimicrobial Properties of Diamond-Like Carbon/Silver Nanocomposite Thin Films Deposited on Textiles: Towards Smart Bandages
Authors: Juknius Tadas , Ružauskas Modestas , Tamulevičius Tomas , Šiugždinienė Rita , Juknienė Indrė , Vasiliauskas Andrius , Jurkevičiūtė Aušrinė , Tamulevičius Sigitas
Published: May 2016

In the current work, a new antibacterial bandage was proposed where diamond-like carbon with silver nanoparticle (DLC:Ag)-coated synthetic silk tissue was used as a building block. The DLC:Ag structure, the dimensions of nanoparticles, the silver concentration and the silver ion release were studied systematically employing scanning electron microscopy, energy dispersive X-ray spectroscopy and atomic absorption spectroscopy, respectively. Antimicrobial properties were investigated using microbiological tests (disk diffusion method and spread-plate technique). The DLC:Ag layer was stabilized on the surface of the bandage using a thin layer of medical grade gelatin and cellulose. Four different strains of Staphylococcus aureus extracted from humans’ and animals’ infected wounds were used. It is demonstrated that the efficiency of the Ag+ ion release to the aqueous media can be increased by further RF oxygen plasma etching of the nanocomposite. It was obtained that the best antibacterial properties were demonstrated by the plasma-processed DLC:Ag layer having a 3.12 at % Ag surface concentration with the dominating linear dimensions of nanoparticles being 23.7 nm. An extra protective layer made from cellulose and gelatin with agar contributed to the accumulation and efficient release of silver ions to the aqueous media, increasing bandage antimicrobial efficiency up to 50% as compared to the single DLC:Ag layer on textile.
Design and Validity of Randomized Controlled Dental Restorative Trials
Authors: Göstemeyer Gerd , Blunck Uwe , Paris Sebastian , Schwendicke Falk
Published: May 2016

Background: The evidence stemming from trials on restorative materials is shaped not only by trial findings, but also trial design and validity. We aimed to evaluate both aspects in randomized controlled dental restorative trials published from 2005–2015. Methods: Using systematic review methodology, we retrieved trials comparing restorative or adhesive dental materials. Two authors independently assessed design, risk of bias, registration status, and findings of trials. Descriptive and regression analyses were performed. Results: 114 studies on 15,321 restorations placed mainly in permanent teeth of 5232 patients were included. Per trial, the median number of patients was 37 (25th/75th percentiles: 30/51). Follow-up was 24 (20/48) months. Seventeen percent of trials reported on sample size calculations, 2% had been registered. Most trials (90%) used US Public Health Service (USPHS) criteria, and had a high risk of bias. More recent trials were more likely to have been registered, to have reported on sample size calculations, to be of low risk of bias, and to use other than USPHS-criteria. Twenty-three percent of trials yielded significant differences between groups. The likelihood of such differences was significantly increased in older studies, studies with potential reporting bias, published in journals with high impact factor (>2), longer follow-up periods, and not using USPHS-criteria. Conclusions: The majority of dental restorative trials published from 2005–2015 had limited validity. Risk of bias decreased in more recent trials. Future trials should aim for high validity, be registered, and use defined and appropriate sample sizes, follow-up periods, and outcome measures.