bluuebunny/crossref_metadata_2025 huggingface dataset:
Limited Role of Endogenous Vasopressin/Copeptin in Stimulation of ACTH–Cortisol Secretion during Glucagon Stimulation Test in Humans
Authors: Malicka Katarzyna , Horzelski Wojciech , Lewiński Andrzej , Lewandowski Krzysztof C.
Published: November 2022

Copeptin is a stable part of a vasopressin precursor that closely mirrors arginine vasopressin (AVP) secretion. It is known that AVP/copeptin is also released in response to nonosmotic stimuli, such as stress evoked during anterior pituitary dynamic testing. In order to examine the role of AVP in challenging the hypothalamo-pituitary-adrenal axis, we assessed adrenocorticotropic hormone (ACTH), cortisol, copeptin and growth hormone (GH) during a glucagon stimulation test (GST) in 10 patients with satisfactory initial cortisol concentrations (mean ± SD: 20.34 ± 5.10 µg/dL) and failure to show any further cortisol increment on stimulation. For comparison, we measured copeptin in two subjects during an insulin tolerance test (ITT). During GST, there was an increase in copeptin (p = 0.02, average individual increase of 98%, range 10% to 321%). There was a robust increase in GH (p = 0.002, average increase 3300%), a decline in cortisol (p = 0.02, average decline 21.8%) and a fall in ACTH (p = 0.06). The relative increase in copeptin during ITT (176% and 52.2%) overlapped with increments observed during GST; however, here there was an increase in cortisol (20.45→24.26 µg/dL and 4.23→29.29 µg/dL, respectively). There was a moderate correlation between copeptin and GH concentrations (r = 0.4235, p = 0.0007). These results confirm that AVP is not crucial for ACTH–cortisol stimulation, though it might be an important factor in GH secretion.
Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium
Authors: Sesorova Irina S. , Sesorov Vitaly V. , Soloviev Pavel B. , Lakunin Konstantin Yu. , Dimov Ivan D. , Mironov Alexander A.
Published: November 2022

Atherosclerosis is a complex non-monogenic disease related to endothelial damage in elastic-type arteries and incorrect feeding. Here, using cryodamage of endothelial cells (ECs) of rat abdominal aorta, we examined the role of the EC basement membrane (BM) for re-endothelization endothelial regeneration and its ability to capture low density lipoproteins (LDLs). Regeneration of endothelium induced thickening of the ECBM. Secretion of the BM components occurred in the G2-phase. Multiple regenerations, as well as arterial hypertension and aging, also led to the thickening of the BM. Under these conditions, the speed of re-endothelialization increased. The thick BM captured more LDLs. LDLs formed after overloading of rats with lipids acquired higher affinity to the BM, presumably due to the prolonged transport of chylomicrons through neuraminidase-positive endo-lysosomes. These data provide new molecular and cellular mechanisms of atherogenesis.
Non-Invasive Evaluation of Kidney Elasticity and Viscosity in a Healthy Cohort
Authors: Maralescu Felix-Mihai , Bende Felix , Sporea Ioan , Popescu Alina , Sirli Roxana , Schiller Adalbert , Petrica Ligia , Miutescu Bogdan , Borlea Andreea , Popa Alexandru , Bodea Madalina , Bob Flaviu
Published: November 2022

Introduction: There is currently a lack of published data on kidney elasticity and viscosity. Non-invasive techniques, such as two-dimensional shear-wave elastography (2D-SWE PLUS) and viscosity plane-wave ultrasound (Vi PLUS), have surfaced as new detection methods, which, thanks to efficient processing software, are expected to improve renal stiffness and viscosity measurements. This study aims to be the first one to assess the normal range values in normal renal function subjects and to investigate the factors that impact them. Methods: We conducted a cross-sectional study employing 50 participants (29 women and 21 men) with a mean age of 42.22 ± 13.17, a mean estimated glomerular filtration rate (eGFR) of 97.12 ± 11 mL/min/1.73 m2, a mean kidney length of 10.16 ± 0.66 cm, and a mean body mass index (BMI) of 24.24 ± 3.98. With a C6-1X convex transducer and the Ultra-FastTM software available on the Hologic Aixplorer Mach 30 ultrasound system, we acquired five measurements of renal cortical stiffness and viscosity (achieved from five distinct images in the middle part of the subcapsular cortex) from each kidney. The ten measurements’ median values correlated with the participant’s demographical, biological, and clinical parameters. Results: The mean kidney elasticity was 31.88 ± 2.89 kiloPascal (kPa), and the mean viscosity was 2.44 ± 0.57 Pascal.second (Pa.s) for a mean measurement depth 4.58 ± 1.02 cm. Renal stiffness seemed to be influenced by age (r = −0.7047, p < 0.0001), the measurement depth (r = −0.3776, p = 0.0075), and eGFR (r = 0.6101, p < 0.0001) but not by BMI (r = −0.2150, p = 0.1338), while viscosity appeared to be impacted by age (r = −0.4251, p = 0.0021), eGFR (r = 0.4057, p = 0.0038), the measurement depth (r = −0.4642, p = 0.0008), and BMI (r = −0.3676, p = 0.0086). The results of the one-way ANOVA used to test the differences in the variables among the three age sub-groups are statistically significant for both 2D-SWE PLUS (p < 0.001) and Vi PLUS (p = 0.015). The method found good intra-operator reproducibility for the 2D-SWE PLUS measurements, with an ICC of 0.8365 and a 95% CI of 0.7512 to 0.8990, and for the Vi PLUS measurements, with an ICC of 0.9 and a 95% CI of 0.8515 to 0.9397. Conclusions: Renal stiffness and viscosity screening may become an efficacious, low-cost way to gather supplemental diagnostic data from patients with chronic kidney disease (CKD). The findings demonstrate that these non-invasive methods are highly feasible and not influenced by gender and that their values correlate with renal function and decrease with age progression. Nevertheless, more research is required to ascertain their place in clinical practice.
Evolution of the Fracture Characteristics in a Rockburst under Different Stress Gradients
Authors: Yang Shuaidong , Gao Yueming , Liu Xiqi , Wang Gang , Song Leibo , Bao Chunyan
Published: November 2022

The variation in principal stress ratio and principal stress direction deflection caused by the stress gradient distribution of surrounding rock is one of the reasons leading to different types of strain rockbursts. Two typical rockburst failure modes of brittle gypsum debris are discussed based on the study of the macroscopic and microscopic appearance morphologies under different stress gradients. Based on the acoustic emission characteristic parameter analysis of the Gaussian mixture model (GMM), the evolution of internal crack propagation and the fracture mechanism during the rockburst under different stress gradients were explored. The results are as follows: (1) The generation and intensity of rockburst are related to the loading stress gradient. The larger the stress gradient, the more significant the dynamic phenomenon during the rockburst process. (2) There are obvious differences in the morphology and arrangement of crystals on the fracture surface of rockburst debris under different stress loading paths. The brittle fracture of debris can be divided into flake debris dominated by intergranular tensile fracture and massive debris dominated by transgranular shear fracture. (3) The AE characteristic parameter classification method based on the GMM has good applicability in crack classification. With an increase in the loading stress gradient, the proportion of the shear crack increases gradually, which is the main reason for the enhancement of the rockburst intensity.
T Cells Directed against the Metastatic Driver Chondromodulin-1 in Ewing Sarcoma: Comparative Engineering with CRISPR/Cas9 vs. Retroviral Gene Transfer for Adoptive Transfer
Authors: Xue Busheng , von Heyking Kristina , Gassmann Hendrik , Poorebrahim Mansour , Thiede Melanie , Schober Kilian , Mautner Josef , Hauer Julia , Ruland Jürgen , Busch Dirk H. , Thiel Uwe , Burdach Stefan E. G.
Published: November 2022

Ewing sarcoma (EwS) is a highly malignant sarcoma of bone and soft tissue with early metastatic spread and an age peak in early puberty. The prognosis in advanced stages is still dismal, and the long-term effects of established therapies are severe. Efficacious targeted therapies are urgently needed. Our previous work has provided preliminary safety and efficacy data utilizing T cell receptor (TCR) transgenic T cells, generated by retroviral gene transfer, targeting HLA-restricted peptides on the tumor cell derived from metastatic drivers. Here, we compared T cells engineered with either CRISPR/Cas9 or retroviral gene transfer. Firstly, we confirmed the feasibility of the orthotopic replacement of the endogenous TCR by CRISPR/Cas9 with a TCR targeting our canonical metastatic driver chondromodulin-1 (CHM1). CRISPR/Cas9-engineered T cell products specifically recognized and killed HLA-A*02:01+ EwS cell lines. The efficiency of retroviral transduction was higher compared to CRISPR/Cas9 gene editing. Both engineered T cell products specifically recognized tumor cells and elicited cytotoxicity, with CRISPR/Cas9 engineered T cells providing prolonged cytotoxic activity. In conclusion, T cells engineered with CRISPR/Cas9 could be feasible for immunotherapy of EwS and may have the advantage of more prolonged cytotoxic activity, as compared to T cells engineered with retroviral gene transfer.
Special Issue: “New Diagnostic and Therapeutic Tools against Multidrug-Resistant Tumors (STRATAGEM Special Issue, EU-COST CA17104)”
Authors: Vasconcelos M. Helena , Passirani Catherine , Riganti Chiara
Published: November 2022

Cancer drug resistance, either intrinsic or acquired, often causes treatment failure and increased mortality [...]
Clinical and Epidemiological Presentation of COVID-19 among Children in Conflict Setting
Authors: Dar Iang Maureen , Hassan Ola El Hajj , McGowan Maureen , Basaleem Huda , Al-Sakkaf Khaled , Jahn Albrecht , Dureab Fekri
Published: November 2022

Background: This study aims to describe the observable symptoms of children with COVID-19 infection and analyze access to real-time polymerase chain reaction (RT-PCR) testing among children seeking care in Yemen. Method: In the period of March 2020–February 2022, data were obtained from 495 children suspected to have been infected with COVID-19 (from a larger register of 5634 patients) from the Diseases Surveillance and Infection Control Department at the Ministry of Public Health and Population in Aden, Yemen. Results: Overall, 21.4% of the children with confirmed COVID-19 infection were asymptomatic. Fever (71.4%) and cough (67.1%) were the most frequently reported symptoms among children, and children were less likely to have fever (p < 0.001), sore throat (p < 0.001) and cough (p < 0.001) compared to adults. A lower frequency of COVID-19-associated symptoms was reported among children with positive RT-PCR tests compared to children with negative tests. A lower rate of testing was conducted among children (25%) compared to adults (61%). Fewer tests were carried out among children <5 years (11%) compared to other age groups (p < 0.001), for children from other nationalities (4%) compared to Yemeni children (p < 0.001) and for girls (21%) compared to boys (30%) (p < 0.031). Conclusion: Understanding and addressing the cause of these disparities and improving guidelines for COVID-19 screening among children will improve access to care and control of the COVID-19 pandemic.
Assessing Pretransplant and Posttransplant Therapy Response in Multiple Myeloma Patients
Authors: Potre Cristina , Borsi Ema , Potre Ovidiu , Samfireag Miruna , Costachescu Dan , Cerbu Bianca , Bratosin Felix , Secosan Cristina , Negrean Rodica Anamaria
Published: November 2022

Multiple myeloma (MM) is a hematologic cancer defined by an abnormal development of clonal plasma cells in the bone marrow, releasing vast quantities of immunoglobulins and different proteins. In the majority of patients, MM remains incurable despite decades of medical improvement and a number of treatment breakthroughs. Frontline standard-of-care has little long-term success, with the majority of patients eventually relapsing, although the overall progression-free survival (PFS) has improved significantly in the last ten years. Patients who are eligible for a transplant have the highest PFS rate at 5 years, depending on medication response and other various factors that are yet to be discovered. Therefore, the current study aimed to evaluate the response to VCD (bortezomib, cyclophosphamide, dexamethasone) and VTD (bortezomib, thalidomide, dexamethasone) used as pretransplant regimens, as well as to compare responses between thalidomide and lenalidomide used as maintenance therapy posttransplant. This retrospective study was performed on a group of 105 hospitalized patients in the Hematology Department of the Timisoara Municipal Emergency Clinical Hospital between January 2016 and December 2021. Data was collected from the paper records of patients with MM who were under-followed. The treatment regimens used as induction therapy were either VCD or VTD if cyclophosphamide was contraindicated. Of the 105 patients, 27 became eligible for bone marrow transplantation. Furthermore, they received maintenance therapy which was based on either lenalidomide with dexamethasone or thalidomide with dexamethasone. Of the 62 patients treated with VTD, 17.7% were in complete remission before stem cell transplantation. Of the 43 patients treated with VCD, 37.2% were in complete remission. The 5-year mean progression-free survival (PFS) in the entire cohort was better in the group treated with the VTD regimen (31.6 vs. 27.2 months). However, in the 27 patients undergoing maintenance after ASCT, the PFS with thalidomide was 35.5 months (95% CI = 27–42), while the PFS rate in those receiving maintenance treatment with lenalidomide was 46.1 months (95% CI = 20–73). VCD proved to be superior to VTD in inducing complete pretransplant responses. Regarding maintenance therapy, patients from the lenalidomide group had superior responses compared with those under thalidomide.
Everything You Always Wanted to Know about Sarcopenia but Were Afraid to Ask: A Quick Guide for Radiation Oncologists (impAct oF saRcopeniA In raDiotherapy: The AFRAID Project)
Authors: Medici Federica , Rizzo Stefania , Buwenge Milly , Arcelli Alessandra , Ferioli Martina , Macchia Gabriella , Deodato Francesco , Cilla Savino , De Iaco Pierandrea , Perrone Anna Myriam , Strolin Silvia , Strigari Lidia , Ravegnini Gloria , Bazzocchi Alberto , Morganti Alessio G.
Published: November 2022

Sarcopenia (SP) is a syndrome characterized by age-associated loss of skeletal muscle mass and function. SP worsens both acute and late radiation-induced toxicity, prognosis, and quality of life. Myosteatosis is a pathological infiltration of muscle tissue by adipose tissue which often precedes SP and has a proven correlation with prognosis in cancer patients. Sarcopenic obesity is considered a “hidden form” of SP (due to large fat mass) and is independently related to higher mortality and worse complications after surgery and systemic treatments with worse prognostic impact compared to SP alone. The evaluation of SP is commonly based on CT images at the level of the middle of the third lumbar vertebra. On this scan, all muscle structures are contoured and then the outlined surface area is calculated. Several studies reported a negative impact of SP on overall survival in patients undergoing RT for tumors of the head and neck, esophagus, rectum, pancreas, cervix, and lung. Furthermore, several appetite-reducing side effects of RT, along with more complex radiation-induced mechanisms, can lead to SP through, but not limited to, reduced nutrition. In particular, in pediatric patients, total body irradiation was associated with the onset of SP and other changes in body composition leading to an increased risk of cardiometabolic morbidity in surviving adults. Finally, some preliminary studies showed the possibility of effectively treating SP and preventing the worsening of SP during RT. Future studies should be able to provide information on how to prevent and manage SP before, during, or after RT, in both adult and pediatric patients.
A Rare Case of Mandibular Aspergillus Osteomyelitis in an Immunocompetent Patient
Authors: Faustino Isabel , Ramos Joab , Mariz Bruno , Papadopoulou Erofili , Georgaki Maria , Nikitakis Nikolaos , Vargas Pablo , Santos-Silva Alan , Lopes Marcio
Published: November 2022

Aspergillosis is a fungal infection caused by Aspergillus species, which is contracted through spores that colonize the respiratory tract, causing rhinosinusitis and pulmonary infections. Oral aspergillosis is rare and, when present, may cause soft tissue and bone destruction, generally in immunodeficient patients. Mandibular Aspergillus osteomyelitis is even rarer, with few cases reported in the literature. A 57-year-old Caucasian woman was referred for the evaluation of painful recurrent swelling in the anterior mandibular alveolar ridge, with purulent drainage, previously treated with multiple surgical debridement procedures and antibiotics without success. The patient was otherwise systemically healthy. Surgical debridement was performed and histopathological examination showed osteomyelitis associated with Aspergillus species. Therapy with oral itraconazole (400 mg per day) was administered for 3 months, resulting in complete resolution. No recurrence was detected after 15 years of follow-up. The patient was rehabilitated with dental implants. In conclusion, non-bacterial microorganisms, such as Aspergillus, should be considered in cases of mandibular osteomyelitis that do not heal after surgical debridement and antibiotic therapy.
2-Mbps Power-Line Communication Transmitter Based on Switched Capacitors for Automotive Networks
Authors: D’Aniello Federico , Ott Andreas , Baschirotto Andrea
Published: November 2022

Nowadays, automotive wire harnesses have become very complex systems as the number of electronic components and Electronic Control Units (ECUs) inside a vehicle have increased dramatically. To manage this complexity, and to support the automotive systems of tomorrow, new communication techniques need to be investigated. In this work, a direct modulated Power-Line Communication (PLC) method is proposed to drastically reduce the number of interconnections and give advantages in cost, complexity, and weight. Two transmitter topologies based on a Switching Capacitor approach are presented and implemented in a Test Chip fabricated in a 180 nm HV-CMOS SOI technology. The proposal is validated by communication tests connecting the designed chip and a discrete component-based demonstrator receiver through an unshielded twisted pair cable. Compared to commercially available solutions, the proposed approach can reach a data rate of 2 Mbps, making it able to implement high-speed event-driven networks, such as the Controller Area Network (CAN).
Experimental Study of Oil Non-Condensable Gas Pyrolysis in a Stirred-Tank Reactor for Catalysis of Hydrogen and Hydrogen-Containing Mixtures Production
Authors: Kolenchukov Oleg A. , Bashmur Kirill A. , Bukhtoyarov Vladimir V. , Kurashkin Sergei O. , Tynchenko Vadim S. , Tsygankova Elena V. , Sergienko Roman B. , Kukartsev Vladislav V.
Published: November 2022

The present study is focused on improving the technology for deep oil sludge processing by pyrolysis methods, considered to be the most promising technology for their environmentally friendly utilization, in which a significant yield of fuel products is expected. The technology developed by the authors of this study is a two-stage process. The first stage, pyrolysis of oil sludge, was investigated in previous papers. A significant yield of non-condensable gases was obtained. This paper presents a study of the second stage of complex deep processing technology—pyrolysis of non-condensable gases (purified propane) using a stirrer with the help of the developed experimental setup. The expected benefit of using the stirrer is improved heat transfer due to circumferential and radial-axial circulation of the gas flow. The effect of a stirrer on the yield of final target decomposition products—H2-containing mixtures and H2 generated during non-catalytic (medium-temperature) and catalytic pyrolysis of non-condensable gases obtained by pyrolysis of oil sludge are estimated. Ni catalyst was used for catalytic pyrolysis. The study shows that the application of the stirrer leads to increasing in H2-containing mixtures and H2 concentrations. In particular, during the whole reaction time (10 h), the average H2 concentration in pyrolysis gas during catalytic pyrolysis increased by ~5.3%. In this case, the optimum reaction time to produce H2 was 4 h. The peak H2 concentration in the pyrolysis gas at reaction temperature 590 ± 10 °C was: 66.5 vol. % with the stirrer versus 62 vol. % without the stirrer with an error of ±0.4 %. A further increase in reaction time is cost-effective in order to obtain H2-containing mixtures.
Sensitivity of the Agribusiness Sector to Sudden Changes in the Prices of Energy Carriers on the Example of Poland: Current State and Challenges
Authors: Gołasa Piotr , Bieńkowska-Gołasa Wioletta , Golonko Magdalena , Trębska Paulina , Gradziuk Piotr , Gromada Arkadiusz , Wysokiński Marcin
Published: November 2022

The article provides an overview of various studies on energy consumption in agriculture. The focus was in particular on the sensitivity of farms and agribusiness to fluctuations in the prices of energy carriers, which is currently a problem for the entire food industry. In addition to the prices of direct energy carriers, biofuels, the EU ETS system, and fertilizers were identified as the main areas that play a key role in shaping the direct and indirect impact on the economic performance of agricultural producers. Moreover, it was presented how sensitive the food sector is to the limitations of fertilizer production, during which by-products necessary in food processing are produced. The production of fertilizers consumes almost half of the energy used in agriculture, but the lack of fertilizers means yield drops of 30–50%. Therefore, high energy prices and disruptions in its supply are a threat to the food security of a country, both from the perspective of agriculture and processing. Based on the literature review, research gaps have been identified that should guide future research.
The Control of Shale Composition on the Pore Structure Characteristics of Lacustrine Shales: A Case Study of the Chang 7 Member of the Triassic Yanchang Formation, Ordos Basin, North China
Authors: Liu Bei , Teng Juan , Li Chen , Li Baoqing , Bie Shizhen , Wang Yinlong
Published: November 2022

The pore structure characteristics of shales are controlled by their mineral and organic matter compositions. However, the contributions of different components to the pore structure characteristics of lacustrine shales remain poorly understood. In this study, fifteen Chang 7 Member shales of the Yanchang Formation, Ordos Basin, were investigated through total organic carbon (TOC), X-ray diffraction (XRD), scanning electron microscopy (SEM), and low-pressure N2 and CO2 adsorption analyses to study the control of shale composition on the pore structure characteristics of lacustrine shales. The results show that the average TOC content of the Chang 7 Member shales is 9.63 wt.%. XRD analysis shows that minerals in the Chang 7 Member shales consist of quartz, feldspars, clay minerals, and pyrite. The clay minerals were dominated by illite, chlorite, and interstratified illite/smectite. The mesopore characteristics of the Chang 7 Member shales and micropore characteristics of organic-lean shales are mainly controlled by clay minerals, whereas the micropore characteristics of organic-rich samples are controlled by both clay minerals and organic matter. SEM observations show that the phyllosilicate framework pores are the main pore type in the Chang 7 Member shales. The results of this study provide important insights into compositional control on the pore structure characteristics of lacustrine shales.
Shale Microstructure Characteristics under the Action of Supercritical Carbon Dioxide (Sc-CO2)
Authors: Yu Chunsheng , Zhao Xiao , Jiang Qi , Lin Xiaosha , Gong Hengyuan , Chen Xuanqing
Published: November 2022

Supercritical carbon dioxide (SC-CO2) is suitable to extract low-polar organics and to assist in the dissolution of pores and fractures in shale. In this work, we investigate the effect of temperature on the structure of five shale samples via high pressure reaction assisted with SC-CO2. Shale samples were analyzed using X-ray diffraction, field emission scanning electron microscopy, and ImageJ software. Due to the extraction of CO2, after Sc-CO2 treatment, carbonate and clay content decreased, while quartz and plagioclase increased slightly, which improved gas and oil flow in microscopic pores and shale cracks. Shale samples showed an increase in surface fracture area as experimental temperature increased. Since Sc-CO2 fluid density and solubility increase with temperature, more organics can be extracted from shale pores and fractures, resulting in newly formed pores and fractures. As a result, the threshold temperature for shale high-temperature Sc-CO2 cracking was confirmed to be 400 °C, and the fracture area increased by more than 45% at this temperature. Based on the findings of this study, Sc-CO2 technology can be used to potentially recover low-maturity shale oil efficiently.
Mechanical Properties of Wood Prediction Based on the NAGGWO-BP Neural Network
Authors: Ma Wei , Wang Wei , Cao Ying
Published: November 2022

The existing original BP neural network models for wood performance prediction have low fitting accuracy and imprecise prediction results. We propose a nonlinear, adaptive grouping gray wolf optimization (NAGGWO)-BP neural network model for wood performance prediction. Firstly, the original gray wolf optimization (GWO) algorithm is optimized. We propose CPM mapping (the Chebyshev mapping method combined with piecewise mapping followed by mod operation) to generate the initial populations and improve population diversity, and an ‘S’-type nonlinear control parameter is proposed to balance the exploitation and exploration capabilities of the algorithm; an adaptive grouping strategy is also proposed, based on which the wolves are divided into the predator, wanderer, and searcher groups. The improved differential evolution strategy, the stochastic opposition-based learning strategy, and the oscillation perturbation operator are used to update the positions of the wolves in the different groups to improve the convergence speed and accuracy of the GWO. Then, the BP neural network weights and thresholds are optimized using the NAGGWO algorithm. Finally, we separately predicted heat-treated wood’s five main mechanical property parameters using different models. The experimental results show that the proposed NAGGWO-BP model significantly improved the mean absolute error (MAE), the mean square error (MSE), and the mean absolute percentage error (MAPE) of the specimens, compared with the BP, GWO-BP, and TSSA-BP algorithms. Therefore, this model has strong generalization ability and good prediction accuracy and reliability, which can fully meet practical engineering needs.
Characterization of Traditional Chinese Sesame Oil by Using Headspace Solid-Phase Microextraction/Gas Chromatography–Mass Spectrometry, Electronic Nose, Sensory Evaluation, and RapidOxy
Authors: Chen Yan , Fu Yingjie , Li Peng , Xi Hui , Zhao Wuduo , Wang Dingzhong , Mao Jian , Zhang Shusheng , Sun Shihao , Xie Jianping
Published: November 2022

Xiao Mo Xiang You (XMXY) is a traditional Chinese sesame oil variety that is obtained through a hot water flotation process. This unique process gives the oil a unique aroma, health benefits, and excellent product stability. Although XMXY is always the most expensive among all the sesame oil varieties, it is usually used as a flavoring in many traditional Chinese daily food products and is increasingly popular. In order to reveal the characteristics of the oil, the volatile components, sensory evaluation, and oxidation stability of five XMXY samples were, respectively, analyzed by using headspace solid-phase microextraction/gas chromatography–mass spectrometry, an electronic nose, sensory evaluation, and RapidOxy. Comparisons and multidimensional statistical analysis were also carried out to distinguish XMXY from roasted sesame oil (RSO) and cold-pressed sesame oil (CSO) samples. In total, 69 volatiles were identified from XMXY, RSO, and CSO samples. Some compounds possessed high odor activity value (OAV > 1) in XMXY, including heterocyclic compounds, phenols, and sulfur-containing compounds. Additionally, they were also the main volatile components that distinguish XMXY from RSO and CSO. Roasted and nutty aromas were the dominant aroma attributes of XMXY. XMXY had better flavor intensity and oxidation stability than the other two sesame oil samples. These results are very valuable for the quality control and product identification of traditional Chinese sesame oil.
Fabrication and Characterization of Botanical-Based Double-Layered Emulsion: Protection of DHA and Astaxanthin Based on Interface Remodeling
Authors: Sun Mengjia , Chen Hongjian , Geng Fang , Zhou Qi , Hao Qian , Zhang Shan , Chen Yashu , Deng Qianchun
Published: November 2022

Both DHA and astaxanthin, with multiple conjugated double bonds, are considered as health-promoting molecules. However, their utilizations into food systems are restricted due to their poor water solubility and high oxidizability, plus their certain off-smell. In this study, the interactions between perilla protein isolate (PPI) and flaxseed gum (FG) were firstly investigated using multiple spectroscopies, suggesting that hydrophobic, electrostatic force and hydrogen bonds played important roles. Additionally, double-layer emulsion was constructed by layer-by-layer deposition technology and exhibited preferable effects on masking the fishy smell of algae oil. Calcium ions also showed an improving effect on the elasticity modulus of O/W emulsions and was managed to significantly protect the stability of co-delivered astaxanthin and DHA, without additional antioxidants during storage for 21 days. The vegan system produced in this study may, therefore, be suitable for effective delivery of both ω-3 fatty acid and carotenoids for their further incorporation into food systems, such as plant-based yoghourt, etc.
Owl Monkey Alu Insertion Polymorphisms and Aotus Phylogenetics
Authors: Storer Jessica M. , Walker Jerilyn A. , Rewerts Lydia C. , Brown Morgan A. , Beckstrom Thomas O. , Herke Scott W. , Roos Christian , Batzer Mark A.
Published: November 2022

Owl monkeys (genus Aotus), or “night monkeys” are platyrrhine primates in the Aotidae family. Early taxonomy only recognized one species, Aotus trivirgatus, until 1983, when Hershkovitz proposed nine unique species designations, classified into red-necked and gray-necked species groups based predominately on pelage coloration. Recent studies questioned this conventional separation of the genus and proposed designations based on the geographical location of wild populations. Alu retrotransposons are a class of mobile element insertion (MEI) widely used to study primate phylogenetics. A scaffold-level genome assembly for one Aotus species, Aotus nancymaae [Anan_2.0], facilitated large-scale ascertainment of nearly 2000 young lineage-specific Alu insertions. This study provides candidate oligonucleotides for locus-specific PCR assays for over 1350 of these elements. For 314 Alu elements across four taxa with multiple specimens, PCR analyses identified 159 insertion polymorphisms, including 21 grouping A. nancymaae and Aotus azarae (red-necked species) as sister taxa, with Aotus vociferans and A. trivirgatus (gray-necked) being more basal. DNA sequencing identified five novel Alu elements from three different taxa. The Alu datasets reported in this study will assist in species identification and provide a valuable resource for Aotus phylogenetics, population genetics and conservation strategies when applied to wild populations.
Cardiac and Mental Benefits of Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) Diet plus Forest Bathing (FB) versus MIND Diet among Older Chinese Adults: A Randomized Controlled Pilot Study
Authors: Yau Ka-Yin , Law Pui-Sze , Wong Chung-Ngok
Published: November 2022

(1) Background: The Mediterranean-DASH intervention for neurodegenerative delay (MIND) diet and forest bathing (FB) are first-line therapies for controlling hypertension. This study aimed to investigate the combined effects of a MIND diet and FB and MIND diet alone among older Chinese patients with hypertension. (2) Methods: Seventy-two participants aged >50 with stages 1 or 2 hypertension were randomly assigned to the MIND group (n = 23), MIND-FB group (n = 25), or control group (n = 24). Systolic blood pressure (SBP) (primary outcome), point-of-care tests for blood lipid panel and glucose (Glu), anxiety levels, mood states, body mass index (BMI), waist-to-hip ratio (WHR), and body fat percentage (BFP) were measured. (3) Results: After a four-week intervention, the change in SBP revealed no significant differences between the intervention groups, and SBP tended to decrease in the MIND and MIND-FB groups. Total cholesterol, and low-density lipoprotein (LDL-C) were significantly decreased (p < 0.01), Triglycerides (TG) and Glu levels were significantly lower (p < 0.05) in the MIND-FB and MIND groups, and the mean differences for anxiety level and negative mood states were significantly lower (p < 0.00) in MIND-FB group. (4) Conclusions: The results provide preliminary evidence that the MIND diet and FB are good for promoting cardiac and mental health well-being in the Chinese population.
Effect of Longitudinal Practice in Real and Virtual Environments on Motor Performance, Physical Activity and Enjoyment in People with Autism Spectrum Disorder: A Prospective Randomized Crossover Controlled Trial
Authors: Moraes Íbis A. P. , Lima Joyce A. , Silva Nadja M. , Simcsik Amanda O. , Silveira Ana C. , Menezes Lilian D. C. , Araújo Luciano V. , Crocetta Tânia B. , Voos Mariana C. , Tonks James , Silva Talita D. , Dawes Helen , Monteiro Carlos B. M.
Published: November 2022

(1) Background: People with ASD commonly present difficulty performing motor skills and a decline in physical activity (PA) level and low enjoyment of PA. We aimed to evaluate whether longitudinal practice of an activity in virtual and real environments improves motor performance and whether this improvement is transferred to a subsequent practice when changing the environment, promoting PA and providing enjoyment; (2) Methods: People with ASD, aged between 10 and 16 years, were included and distributed randomly into two opposite sequences. The participants performed a 10 session protocol, with five sessions practicing in each environment (virtual or real). Heart rate measurement was carried out and an enjoyment scale was applied; (3) Results: 22 participants concluded the protocol. Sequence A (virtual first) presented an improvement in accuracy and precision and transferred this when changing environment; they also had a greater change in heart rate reserve. The majority of participants reported “fun” and “great fun” levels for enjoyment; (4) Conclusions: The virtual reality activity presented a higher level of difficulty, with greater gains in terms of transference to the real environment. Considering PA, our task provided very light to light activity and the majority of participants enjoyed the task.
Communication Management Processes of Dentists Providing Healthcare for Migrants with Limited Japanese Proficiency
Authors: Imafuku Rintaro , Nagatani Yukiko , Shoji Masaki
Published: November 2022

Low health literacy results in health inequity are linked with poor adherence to medical care. In the globalized Japanese context, the number of migrants with Japanese as a second language is increasing year after year. Since limited Japanese proficiency may pose a greater health risk, dentists are expected to manage cross-cultural communication and provide dental care to foreign patients. This study explored dentists’ experiences of treating patients with limited Japanese proficiencies. Semi-structured interviews were conducted with 11 community dentists and the qualitative data were analyzed through a thematic analysis approach. Their major challenges were classified into three themes—linguistic aspect (e.g., complicated explanation regarding root canal treatment), sociolinguistic aspect (e.g., communication with foreign residents with limited dental knowledge), and sociocultural aspect (e.g., cultural differences in their dental aesthetics and insurance treatment system). Several management strategies were employed, including linguistic accommodation, avoidance of complexities, use of various communication tools, and getting help from others. However, they were unsatisfied with their practice because they could not understand the patients’ psychosocial aspects due to incomplete communication. These findings provided insights into dentists’ practice in the globalized context.
Long COVID at Different Altitudes: A Countrywide Epidemiological Analysis
Authors: Izquierdo-Condoy Juan S. , Fernandez-Naranjo Raul , Vasconez-González Eduardo , Cordovez Simone , Tello-De-la-Torre Andrea , Paz Clara , Delgado-Moreira Karen , Carrington Sarah , Viscor Ginés , Ortiz-Prado Esteban
Published: November 2022

Background: Several reports from around the world have reported that some patients who have recovered from COVID-19 have experienced a range of persistent or new clinical symptoms after a SARS-CoV-2 infection. These symptoms can last from weeks to months, impacting everyday functioning to a significant number of patients. Methods: A cross-sectional analysis based on an online, self-reporting questionnaire was conducted in Ecuador from April to July 2022. Participants were invited by social media, radio, and TV to voluntarily participate in our study. A total of 2103 surveys were included in this study. We compared socio-demographic variables and long-term persisting symptoms at low (<2500 m) and high altitude (>2500 m). Results: Overall, 1100 (52.3%) responders claimed to have Long-COVID symptoms after SARS-CoV-2 infection. Most of these were reported by women (64.0%); the most affected group was young adults between 21 to 40 years (68.5%), and most long-haulers were mestizos (91.6%). We found that high altitude residents were more likely to report persisting symptoms (71.7%) versus those living at lower altitudes (29.3%). The most common symptoms were fatigue or tiredness (8.4%), hair loss (5.1%) and difficulty concentrating (5.0%). The highest proportion of symptoms was observed in the group that received less than 2 doses. Conclusions: This is the first study describing post-COVID symptoms’ persistence in low and high-altitude residents. Our findings demonstrate that women, especially those aging between 21–40, are more likely to describe Long-COVID. We also found that living at a high altitude was associated with higher reports of mood changes, tachycardia, decreased libido, insomnia, and palpitations compared to lowlanders. Finally, we found a greater risk to report Long-COVID symptoms among women, those with previous comorbidities and those who had a severer acute SARS-CoV-2 infection.
Is There Agreement and Precision between Heart Rate Variability, Ventilatory, and Lactate Thresholds in Healthy Adults?
Authors: Neves Letícia Nascimento Santos , Gasparini Neto Victor Hugo , Araujo Igor Ziviani , Barbieri Ricardo Augusto , Leite Richard Diego , Carletti Luciana
Published: November 2022

This study aims to analyze the agreement and precision between heart rate variability thresholds (HRVT1/2) with ventilatory and lactate thresholds 1 and 2 (VT1/2 and LT1/2) on a treadmill. Thirty-four male students were recruited. Day 1 consisted of conducting a health survey, anthropometrics, and Cardiopulmonary Exercise Test (CPx). On Day 2, after 48 h, a second incremental test was performed, the Cardiopulmonary Stepwise Exercise Test consisting of 3 min stages (CPxS), to determine VT1/2, LT1/2, and HRVT1/2. One-way repeated-measures ANOVA and effect size (ηp2) were used, followed by Sidak’s post hoc. The Coefficient of Variation (CV) and Typical Error (TE) were applied to verify the precision. Bland Altman and the Intraclass Correlation Coefficient (ICC) were applied to confirm the agreement. HRVT1 showed different values compared to LT1 (lactate, RER, and R-R interval) and VT1 (V̇E, RER, V̇CO2, and HR). No differences were found in threshold 2 (T2) between LT2, VT2, and HRVT2. No difference was found in speed and V̇O2 for T1 and T2. The precision was low to T1 (CV > 12% and TE > 10%) and good to T2 (CV < 12% and TE < 10%). The agreement was good to fair in threshold 1 (VT1, LT1, HRVT1) and excellent to good in T2 (VT1, LT1, HRVT1). HRVT1 is not a valid method (low precision) when using this protocol to estimate LT1 and VT1. However, HRVT2 is a valid and noninvasive method that can estimate LT2 and VT2, showing good agreement and precision in healthy adults.
Evaluation of Potentially Toxic Trace Metals and Associated Health Risk Assessment in Buffalo Milk
Authors: Rafiq Aqsa , Shah Munir H. , Mohany Mohamed , Tahir Adnan Ahmad , Elsadek Mohamed Farouk , Qayyum Muhammad Abdul , Abbasi Arshad Mehmood
Published: November 2022

The contamination of toxic trace metals in the food chain is one of the major threats to human health. Milk is part of a balanced diet, which is essential for proper growth, but the ingestion of contaminated milk may cause chronic health disorders. The present study is focused on the assessment of contamination of toxic trace metals in buffalo milk and the associated health risks to the consumers of Abbottabad, Pakistan. Standard analytical methods were employed to quantify the metal contents in the milk samples collected from various shops and homes in the months from June 2021 to October 2021. Health risk assessment was accomplished by computing estimated daily intake (EDI), health risk index (HRI), target hazard quotient (THQ), hazard index (HI), and target cancer risk (TCR). On a comparative basis, the mean concentration of Cr was found to be highest in both shop and home milk samples (101.3 ± 45.33 and 54.11 ± 24.20 mg/L, respectively), followed by Pb, Zn, Ni, and Cd levels. In buffalo milk collected from homes, the highest concentration of the metals was found in October, followed by July, September, June, and August. In shop milk, the increasing trend of metal contents was July > October > September > June > August. Significantly strong positive relationships were noted between the metal concentrations in the milk samples. Multivariate cluster analysis and principal component analysis exhibited significant anthropogenic contributions of the metals in buffalo milk. Mostly, the EDI and HRI values were exceeding the recommended limits; however, THQ, HI, and TCR showed that the intake of these metals through milk consumption was within the safe limit and thus revealed no significant carcinogenic or non-carcinogenic risks to the consumers. It is high time to ensure the continuous monitoring of organic/inorganic toxins in the milk and concerned authorities should take strict measures to control the contamination of milk and other food products.
Impact of Respiratory Syncytial Virus and Influenza Virus Infection in the Adult Population in Spain between 2012 and 2020
Authors: Heppe-Montero Marco , Gil-Prieto Ruth , del Diego Salas Jorge , Hernández-Barrera Valentín , Gil-de-Miguel Ángel
Published: November 2022

Respiratory syncytial virus (RSV) infection is increasingly recognized as a cause of significant morbidity and mortality in adults. We aimed to estimate the rates of age-specific hospitalization and in-hospital mortality caused by acute lower respiratory tract infections (ALRTIs) in Spain between 2012 and 2020 and to compare the relative impact of RSV and influenza virus infection in adults. We used the discharge reports from the Minimum Basic Data Set to retrospectively analyze hospital discharge data on the basis of the ICD-9-CM and ICD-10-CM diagnosis codes. A total of 1,518,244 patients were hospitalized for ALRTIs, of whom 137,794 (9.1%) were admitted for RSV-related infections and 46,288 (3.0%) for influenza-related infections. In patients aged 60 years or older, the hospitalization rates (per 100,000 population) were estimated at 1.69 (95% CI 1.68–1.70) and 2.72 (95% CI 2.71–2.73) for RSV and influenza patients, respectively. However, in-hospital mortality rates were significantly higher among RSV patients than among influenza patients, 7.91% (95% CI 7.89–7.93) (83.0% of all RSV-related deaths) versus 6.91% (95% CI 6.89–6.93) (85.6% of all influenza-related deaths), respectively (p = 0.007). RSV-associated in-hospital mortality increases exponentially with age, posing a greater risk for older adults, particularly frail and high-risk patients.
The Link between the Demographic and Clinical Factors and Fatigue Symptoms among Rheumatoid Arthritis Patients
Authors: Kozłowska Katarzyna Anna , Formanowicz Dorota , Bączyk Grażyna
Published: November 2022

Rheumatoid arthritis (RA) is a chronic systemic disease of connective tissue with periods of exacerbation and remission. Fatigue is excessive strain throughout the body that is disproportionate or unrelated to an activity or lifestyle. Fatigue is an integral part of RA in most patients. The study aimed to assess the level of fatigue in RA patients and establish the relationship between fatigue and demographic and clinical factors. The study group consisted of 128 RA patients according to European League Against Rheumatism (EULAR) criteria. The Functional Assessment of Chronic Illness Therapy-Fatigue and -Medical Outcomes Study Short Form 36 (SF-36) vitality scores were used to assess the severity of fatigue symptoms. The analyzed variables were gender, age, disease duration, education, marital status, place of residence, work and residence status, pharmacological treatment, pain, morning stiffness, hemoglobin, C-reactive protein (CRP), rheumatoid factor (RF), compression soreness, Richie Articular Index, and DAS28 disease activity. The examined patients experience chronic fatigue—the mean value on the FACIT-F scale was 24.1 ± 9.1 points and on the SF-36 Vitality score was 14.2 ± 1.8 points. There is a relationship between the level of fatigue and pain, long-lasting morning stiffness, active disease, increased soreness of joints, and low hemoglobin values. When analyzing the symptom of fatigue, each patient should be approached individually, using the existing questionnaires or asking key questions to recognize the situation. The presence of fatigue symptoms should be considered during therapy and patient care by searching for and eliminating additional, intensifying stimuli and increasing its level.