bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of circulating microparticles and cytokines in periodontitis associated with diabetes
Authors: Cassiano Bárbara Adelungue , Silveira Ana Luíza Pereira Assunção , Kim Yeon Jung , do Amaral Jônatas Bussador , da Silva Nali Luiz Henrique , Bachi André Luis Lacerda , Resende Leonardo Diniz , Fonseca Francisco Antonio Helfenstein , de Oliveira Izar Maria Cristina , Tuleta Izabela Dorota , Victor Jefferson Russo , Pallos Débora , França Carolina Nunes
Published: August 2024

Background

Periodontitis is a chronic inflammatory condition that affects the supporting tissues of the teeth, and can lead to serious complications such as tooth loss and systemic health problems, including diabetes, which have a bidirectional relationship with periodontitis. Circulating microparticles originate from different cell types after stimuli such as activation or apoptosis. Interleukins are related to processes in the regulation of the immune response, inflammation, and cell growth. This study aimed to evaluate circulating microparticles as well as interleukins in the plasma, at baseline and 1 month after the end of the non-surgical periodontal treatment.

Methods

Samples were collected from 45 patients, with moderate to severe periodontitis with diabetes (N = 25) and without diabetes (N = 20). Microparticles were evaluated in the platelet-poor plasma by flow cytometer. Cytokine levels were evaluated by the enzyme immunoabsorption assay (ELISA).

Results

Higher levels of the pro-inflammatory cytokines were found in the group with diabetes compared to the non-diabetic group both at baseline and 1 month after the end of the treatment. A higher IL-6/IL-10 ratio was found in patients with diabetes compared to the group without diabetes at T0 and T1, whereas an increased IFN-γ/IL-10 ratio was only found at T1 in patients with diabetes in comparison to the group without diabetes. In the group with diabetes, it was verified positive correlations between IL-10 and IL-6 or IFN-γ and a negative correlation between IL-6 and PMP, at T0; in contrast, in the T1, negative correlations were found between TNF-α and IL-10 or PMP. Besides, at T0, it was evidenced positive correlations both between circulating TNF-α and IL-6, and IL-10 and EMP, as well as a negative correlation between IL-10 and PMP in the group with diabetes. In addition, it was observed in T1 positive correlations between levels of TNF-α and IL-6, IFN-γ, or IL-10, and between PMP and IFN-γ, and between EMP and IL-6, TNF-α and IFN-γ in this group.

Conclusion

The results suggest a modulatory effect of the periodontitis associated with diabetes, as well as the periodontal treatment, in the systemic inflammatory status of the participants of the study.
Unveiling the hidden link: fungi and HPV in cervical lesions
Authors: Zhang Yulong , Chen Lingsi , Li Haibo , Zhuang Yiling , Xie Qing , Li Wenwen , Yang Xia , Zheng Xiangqin , Suyu Li , Yi Huan
Published: August 2024

Background

Cervical cancer, primarily driven by high-risk human papillomavirus (HR-HPV) infection, ranks as the second most common cancer globally. Understanding combined infections’ role, including Cervical fungi, is crucial in cervical carcinogenesis. This study aims to explore the potential correlation between HR-HPV, cervical fungi, and cervical cancer, while adjusting for various factors.

Methods

The study population comprised patients undergoing colposcopy and conization due to abnormal cervical screening results. Clinical data including age, gravidity, HPV (human papillomavirus) genotypes, cervical pathology, and p16/Ki67 expression were extracted. Cervical TCT (ThinPrep Pap Test) and HPV testing are utilized for screening cervical lesions, with fungal presence suggested by TCT results. 5,528 participants were included in this study. Statistical analyses investigated associations between HPV/fungi co-infection and cervical lesions, employing multinomial logistic regression and interaction analysis.

Results

Co-infection with fungi and HPV may decrease the risk of cervical lesions compared to HPV infection alone. In the co-infection group, compared with HPV infection alone, the risk of low-grade squamous intraepithelial lesions (LSIL) was reduced by 27% (OR = 0.73, 95% CI: 0.59–0.90), the risk of high-grade squamous intraepithelial lesions (HSIL) was reduced by 35% (OR = 0.65, 95% CI: 0.51–0.82), and the risk of cervical cancer was reduced by 43% (OR = 0.57, 95% CI: 0.35–0.92). The interaction analysis revealed a negative interaction between fungal and HPV infections in the development of cervical cancer (RERI = −6.25, AP = −0.79, SI = 0.52), HSIL (RERI = −19.15, AP = −0.37, SI = 0.72) and LSIL (RERI = −1.87, AP = −0.33, SI = 0.71), suggesting a sub-additive effect, where the combined effect of the two infections was less than the sum of their individual effects. This indicates that fungal infection may attenuate the promoting effect of HPV on cervical lesions. In exploring the potential mechanism, we found that the co-infection group had significantly lower p16 positivity (54.6%) compared to the HPV-only group (60.2%) (p = 0.004), while there was no statistically significant difference in Ki67 positivity.

Conclusion

This study unveils the intricate relationship between cervical fungi and HPV in cervical lesions. Co-infection with fungi and HPV against cervical lesions compared to HPV infection alone, indicating a novel clinical interaction. Lower p16 positivity in co-infection hints at a protective mechanism, urging further exploration.
Predictive modeling of colorectal cancer using exhaustive analysis of microbiome information layers available from public metagenomic data
Authors: Murovec Boštjan , Deutsch Leon , Stres Blaž
Published: August 2024

This study aimed to compare the microbiome profiles of patients with colorectal cancer (CRC, n = 380) and colorectal adenomas (CRA, n = 110) against generally healthy participants (n = 2,461) from various studies. The overarching objective was to conduct a real-life experiment and develop a robust machine learning model applicable to the general population. A total of 2,951 stool samples underwent a comprehensive analysis using the in-house MetaBakery pipeline. This included various data matrices such as microbial taxonomy, functional genes, enzymatic reactions, metabolic pathways, and predicted metabolites. The study found no statistically significant difference in microbial diversity among individuals. However, distinct clusters were identified for healthy, CRC, and CRA groups through linear discriminant analysis (LDA). Machine learning analysis demonstrated consistent model performance, indicating the potential of microbiome layers (microbial taxa, functional genes, enzymatic reactions, and metabolic pathways) as prediagnostic indicators for CRC and CRA. Notable biomarkers on the taxonomy level and microbial functionality (gene families, enzymatic reactions, and metabolic pathways) associated with CRC were identified. The research presents promising avenues for practical clinical applications, with potential validation on external clinical datasets in future studies.
Nitrogen uptake, grain yield, and oil concentration of dwarf castor beans under nitrogen rates and inoculation of rhizobacteria in grasses–legumes rotation
Authors: Gato Isabela Martins Bueno , Oliveira Carlos Eduardo da Silva , Jalal Arshad , Moreira Vitória de Almeida , Hashem Amr H. , de Lima Bruno Horschut , Leite Gabriel da Silva , Al-Askar Abdulaziz A. , Freitas Leandro Alves , AbdElgawad Hamada , Ferreira Selton Vinicius Domingos , Santana Leticia de Jesus , Bastos Andréa de Castro , Galindo Fernando Shintate , Zoz Tiago , Teixeira Filho Marcelo Carvalho Minhoto
Published: August 2024

Introduction

Plant growth-promoting bacteria (PGPB) have been primarily studied for atmospheric nitrogen (N) fixation but they also have the capacity to improve nutrition and yield of crop plants.

Methods

Therefore, the objective of this research was to investigate the effects of inoculation with PGPB in association with different N rates on N uptake, grain yield, and oil concentration of dwarf castor beans in succession to legumes and grasses in Ilha Solteira, Brazil. The treatments consisted of N rates (0 to 180 kg ha−1 of N) and inoculation with three plant growth-promoting bacteria (Azospirillum brasiliense, Bacillus subtilis, and Pseudomonas fluorescens, applied by leaf) and a control with no-inoculation.

Results

The grain and oil yields of castor beans were increased by 20 and 40% at a rate of 103 kg ha−1 of N in succession to grasses as compared to without N application. In addition, the grain yield of castor bean after legumes was increased by 28, 64, and 40% with estimated rates of 97, 113, and 92 kg ha−1 of N in combination with inoculations of A. brasilense, B. subtilis, and P. fluorescens as compared to without N application, respectively. Shoot, grain, and total N uptake were improved with foliar inoculation of A. brasilense, B. subtilis, and P. fluorescens at the N rates of 45, 90, and 135 kg ha−1, respectively.

Discussion and conclusions

Topdressing of N at the rate of 103 kg ha−1 and foliar inoculation in succession to grasses and 180 kg ha−1 of N without the effect of foliar inoculation in succession to legumes are recommended for higher grain and oil yield of castor beans. Foliar inoculations with A. brasilense, B. subtilis, and P. fluorescens increased grain yield under reduced use of N fertilizer by 44, 37, and 49% in dwarf castor cultivation in succession to legumes, potentially contributing to sustainable agriculture.
Digital Clock and Recall: a digital, process-driven evolution of the Mini-Cog
Authors: Gomes-Osman Joyce , Borson Soo , Toro-Serey Claudio , Banks Russell , Ciesla Marissa , Jannati Ali , Morrow W. Isaiah , Swenson Rod , Libon David , Bates David , Showalter John , Tobyne Sean , Pascual-Leone Alvaro
Published: August 2024

Introduction

Alzheimer’s disease and related dementias (ADRD) represent a substantial global public health challenge with multifaceted impacts on individuals, families, and healthcare systems. Brief cognitive screening tools such as the Mini-Cog© can help improve recognition of ADRD in clinical practice, but widespread adoption continues to lag. We compared the Digital Clock and Recall (DCR), a next-generation process-driven adaptation of the Mini-Cog, with the original paper-and-pencil version in a well-characterized clinical trial sample.

Methods

DCR was administered to 828 participants in the Bio-Hermes-001 clinical trial (age median ± SD = 72 ± 6.7, IQR = 11; 58% female) independently classified as cognitively unimpaired (n = 364) or as having mild cognitive impairment (MCI, n = 274) or dementia likely due to AD (DLAD, n = 190). MCI and DLAD cohorts were combined into a single impaired group for analysis. Two experienced neuropsychologists rated verbal recall accuracy and digitally drawn clocks using the original Mini-Cog scoring rules. Inter-rater reliability of Mini-Cog scores was computed for a subset of the data (n = 508) and concordance between Mini-Cog rule-based and DCR scoring was calculated.

Results

Inter-rater reliability of Mini-Cog scoring was good to excellent, but Rater 2’s scores were significantly higher than Rater 1’s due to variation in clock scores (p < 0.0001). Mini-Cog and DCR scores were significantly correlated (τB = 0.71, p < 0.0001). However, using a Mini-Cog cut score of 4, the DCR identified more cases of cognitive impairment (n = 47; χ2 = 13.26, p < 0.0005) and Mini-Cog missed significantly more cases of cognitive impairment (n = 87). In addition, the DCR correctly classified significantly more cognitively impaired cases missed by the Mini-Cog (n = 44) than vice versa (n = 4; χ2 = 21.69, p < 0.0001).

Discussion

Our findings demonstrate higher sensitivity of the DCR, an automated, process-driven, and process-based digital adaptation of the Mini-Cog. Digital metrics capture clock drawing dynamics and increase detection of diagnosed cognitive impairment in a clinical trial cohort of older individuals.
The AST/ALT ratio predicts survival and improves oncological therapy decisions in patients with non-small cell lung cancer receiving immunotherapy with or without radiotherapy
Authors: Zhang Yanyan , Zhang Jingxin , Shang Shijie , Ma Jiachun , Wang Fei , Wu Meng , Yu Jinming , Chen Dawei
Published: August 2024

Background and purpose

Immunotherapy, with or without radiotherapy (iRT or ICIs-nonRT), is the standard treatment for non–small cell lung cancer (NSCLC). Nonetheless, the response to the treatment varies among patients. Given the established role of aspartate aminotransferase/alanine transaminase (AST/ALT) ratio in predicting cancer prognosis, we sought to identify whether the pre-treatment AST/ALT ratio has the potential to serve as a prognostic factor for NSCLC patients receiving ICIs-nonRT and iRT.

Materials and methods

We retrospectively analyzed NSCLC patients who received immunotherapy between April 2018 and March 2021. Patients were classified into iRT group and ICIs-nonRT group and further classified based on AST/ALT ratio cut-off values. The Kaplan-Meier (KM) method estimated the time-to-event endpoints (progression-free survival (PFS) and overall survival (OS)

Results

Of the cohort, 239 underwent ICIs-nonRT and 155 received iRT. Higher AST/ALT ratios correlated with worse outcomes in the ICIs-nonRT group but indicated better outcomes in those who received iRT. Multivariate analysis validated AST/ALT ratio as an independent prognostic factor. For AST/ALT ratios between 0.67-1.7, both ICIs-nonRT and iRT yielded similar treatment outcomes; with AST/ALT ratios greater than 1.7, iRT could be a more favorable treatment option (P=0.038). Conversely, for ratios less than 0.67, ICIs-nonRT could be a more favorable treatment option (P=0.073).

Conclusions

The pre-treatment AST/ALT ratio demonstrates potential as a prognostic marker for treatment outcomes in NSCLC patients receiving either ICIs-nonRT or iRT. This finding could help guide clinicians in selecting more effective treatment protocols, thereby enhancing patient prognosis.
Clinical utility of repeated rebiopsy for EGFR T790M mutation detection in non-small cell lung cancer
Authors: Lee Eun Hye , Kwak Se Hyun , Kim Kyeong Yeon , Kim Chi Young , Lee Sang Hoon , Heo Seok-Jae , Chang Yoon Soo , Kim Eun Young
Published: August 2024

Purpose

In cases where rebiopsy fails to find the epidermal growth factor receptor (EGFR) T790M mutation, the criteria for selecting patients for repeated rebiopsy remains unclear. This study aimed to assess the impact of repeated rebiopsy on T790M mutation detection in non-small cell lung cancer (NSCLC) patients.

Methods

Patients with advanced EGFR-mutated NSCLC between January 2018 and December 2021 at three-referral hospitals in South Korea underwent retrospective review. Of 682 patients who had rebiopsy after disease progression, T790M mutation status was assessed in plasma circulating tumor DNA (ctDNA) and/or tumor tissues.

Results

The overall T790M positivity rate increased from 40.8% after the first rebiopsy to 52.9% following multiple rebiopsies in the entire study population. Longer duration of initial EGFR TKI use (OR 1.792, ≥8 months vs. <8 months, p=0.004), better EGFR TKI responses (OR 1.611, complete or partial response vs. stable disease, p=0.006), presence of bone metastasis (OR 2.286, p<0.001) were correlated with higher T790M positivity. Longer EGFR TKI use and better responses increased T790M positivity in repeated tissue rebiopsy, while bone metastasis favored liquid rebiopsy. Additionally, T790M status has been shown to be positive over time through repeated rebiopsies ranging from several months to years, suggesting its dynamic nature.

Conclusion

In this study, among patients who initially tested negative for T790M in rebiopsy, repeated rebiopsies uncovered an additional 23.5% T790M positivity. Particularly, it is suggested that repeated rebiopsies may be valuable for patients with prolonged EGFR TKI usage, better responses to treatment, and bone metastasis.
Intratumoral injection and retention hold promise to improve cytokine therapies for cancer
Authors: Sauer Karsten , Rakhra Kavya , Wu Kaida , Mehta Naveen K. , Michaelson Jennifer S. , Baeuerle Patrick A.
Published: August 2024

As powerful activators of the immune system, cytokines have been extensively explored for treating various cancers. But despite encouraging advances and some drug approvals, the broad adoption of cytokine therapies in the clinic has been limited by low response rates and sometimes severe toxicities. This in part reflects an inefficient biodistribution to tumors or a pleiotropic action on bystander cells and tissues. Here, we first review these issues and then argue for the intratumoral delivery of engineered cytokine fusion proteins that have been optimized for tumor retention as a potential solution to overcome these limitations and realize the potential of cytokines as highly effective therapeutics for cancer.
Relaxing at work: does virtual reality work? An exploratory study among employees in their workplace
Authors: Michinov Estelle , Dodeler Virginie , Adam Hugo , Michinov Nicolas
Published: August 2024

Introduction

The development of virtual reality provides new opportunities for conducting relaxing activities in a simulated environment. Although studies on the effects of VR-based relaxation are developing, they have mostly been performed with students, and more rarely with employees.

Methods

This study aimed to examine the effects of VR relaxation exercises with employees. Forty-seven employees were divided into two conditions: Virtual Reality (n = 25) and 2D video (n = 22).

Results

The results indicate that relaxing activities reduced stress and negative emotions, while increasing perceived restoration using VR and 2D video. The acceptability and user experience were higher only in the VR condition. VR relaxation provides a sense of disconnection from the workplace that allows employees to increase the pleasant experience.

Discussion

The 2D solution, while less immersive, can be an alternative for employees for whom VR is not recommended. The question of inclusive digital tools is a key issue in the quality of working life programs.
Optical clearing: an alternative sample preparation method for propagation based phase contrast μCT
Authors: Sagar Md Motiur Rahman , Svetlove Angelika , D’Amico Lorenzo , Pinkert-Leetsch Diana , Missbach-Guentner Jeannine , Longo Elena , Tromba Giuliana , Bohnenberger Hanibal , Alves Frauke , Dullin Christian
Published: August 2024

Synchrotron radiation propagation-based phase-contrast micro-computed tomography (SRμCT) has been used increasingly for detailed characterization of tissue specimens, alternative to classical histology. It allows rapid and non-destructive three-dimensional (3D) virtual histology of unstained specimens. Although many studies show that standard protocols for formalin-fixation and paraffin-embedded (FFPE) tissue specimens are well suited for propagation based phase-contrast imaging (PBI), refinement of sample preparation protocols for this relatively recent development is still in its early stage. There are several limitations using FFPE blocks including air-bubble inclusion, and crack formation, which affect the quality of the micro-CT scans. In this study we demonstrate that optical clearing of colon cancer specimen followed by embedding in Phytagel, as an alternative sample preparation protocol yields comparable PBI micro-CT image quality and mitigates the aforementioned drawbacks of FFPE specimens. Moreover, since single-distance phase retrieval algorithms for PBI image reconstruction expect scanned materials to be either weakly-absorbing or having a fixed ratio between absorption and phase shift, we hypothesize that optical clearing will result in specimens that are well in line with these mathematical assumption of the applied phase retrieval algorithm (homogeneous form of the Transport-of-Intensity equation TIE-HOM). In addition, we show that classical histological analysis is still possible after re-embedding of the specimen in paraffin. Thus, the proposed novel workflow to scan optical cleared specimens using SRμCT can be used in combination with light sheet microscopy and can be integrated in a classical pipeline for pathological tissue characterization.
Genetic markers identify duplicates in Nordic potato collections
Authors: Chrominski Pawel , Carlson-Nilsson Ulrika , Palmé Anna , Kirk Hanne Grethe , Asdal Åsmund , Ansebo Lena
Published: August 2024

Introduction

The first small scale cultivation of potatoes in the Nordic countries began roughly 300 years ago, and later became an important staple food in the region. Organized conservation efforts began in the 1980s, and today, potato landraces, improved varieties, and breeding lines are conserved in genebanks at the Nordic Genetic Resource Center (NordGen), Sweden, and the Norwegian Genetic Resource Centre (NGS), Norway, as well as at potato breeding companies across Nordic countries. All these collections house a diverse array of genotypes with local names and local growing histories from the whole region. However, the presence of duplicates, and inconsistent naming has led to confusion.

Methods

In this study, 198 accessions of cultivated potato (Solanum tuberosum L.) have been genotyped with 62 microsatellite (SSR) markers. The analyzed accessions came from three collections: 43 accessions from the Danish Potato Breeding Foundation in Vandel (LKF-Vandel), 90 from NordGen and 65 from NGS.

Results and discussion

The genetic analysis revealed 140 unique potato genotypes and 31 groups/clusters of duplicates, most of which contained duplicate pairs and the others three to ten accessions. Several accessions with distinct names were genetically identical or very similar, suggesting historical sharing, and regional distribution of seed potatoes, leading to the emergence of diverse local names. Moreover, many improved varieties from early potato breeding were revealed to have duplicates that have been considered Nordic landraces. Furthermore, potato accessions with identical names but originating from different collections were confirmed to be duplicates. These findings have already influenced management decisions and will further improve management practices for Nordic potato collections. Additionally, this new knowledge will benefit Nordic potato breeding efforts and allow for the dissemination of more accurate information to other users of potato diversity.
Genomic analyses of agronomic traits in tea plants and related Camellia species
Authors: Duan Shengchang , Yan Liang , Shen Zongfang , Li Xuzhen , Chen Baozheng , Li Dawei , Qin Hantao , Meegahakumbura Muditha K. , Wambulwa Moses C. , Gao Lianming , Chen Wei , Dong Yang , Sheng Jun
Published: August 2024

The genus Camellia contains three types of domesticates that meet various needs of ancient humans: the ornamental C. japonica, the edible oil-producing C. oleifera, and the beverage-purposed tea plant C. sinensis. The genomic drivers of the functional diversification of Camellia domesticates remain unknown. Here, we present the genomic variations of 625 Camellia accessions based on a new genome assembly of C. sinensis var. assamica (‘YK10’), which consists of 15 pseudo-chromosomes with a total length of 3.35 Gb and a contig N50 of 816,948 bp. These accessions were mainly distributed in East Asia, South Asia, Southeast Asia, and Africa. We profiled the population and subpopulation structure in tea tree Camellia to find new evidence for the parallel domestication of C. sinensis var. assamica (CSA) and C. sinensis var. sinensis (CSS). We also identified candidate genes associated with traits differentiating CSA, CSS, oilseed Camellia, and ornamental Camellia cultivars. Our results provide a unique global view of the genetic diversification of Camellia domesticates and provide valuable resources for ongoing functional and molecular breeding research.
Psychological status of medical security teams in Winter Olympic Games and Paralympics under COVID-19
Authors: Zhu Xiaoyu , Zhu Yu , Qi Zhiwei , Li Ran , Tan Yunlong , Yang Zhongwei
Published: August 2024

Background

Medical security work for the 2022 Beijing Winter Olympic and Paralympics faced enormous challenges under COVID-19. This study aimed to investigate the mental status of those medical team members to provide a reference for scientifically implementing medical security services for large-scale events.

Methods

In this prospective cohort study, the Patient Health Questionnaire-9, Self-rating Anxiety Scale, General Self-Efficacy Scale, and Psychological Questionnaire for Emergent Events of Public Health (PQEEPH) were administered to 145 members of the medical team. A generalized mixed linear model was used to analyze the impact of work duration, position, on/off rotation, and gender on psychological status.

Results

Work duration significantly impacted depression, anxiety, self-efficacy, and all dimensions of PQEEPH. Women scored higher than men in the PQEEPH dimensions of depression, neurasthenia, fear, and total score. Working status affected the dimensions of depression, neurasthenia, and total score. Deterioration in emotional state became apparent in the fourth week and recovered 1 week after the task concluded, while self-efficacy decreased from beginning to end.

Conclusion

All the medical team members experienced emotional deterioration and decreased self-efficacy in medical security tasks. To maintain a medical team’s psychological wellbeing during large-scale activities, rotation times should be set reasonably, and adequate mental health services should be provided.
Mapping competency profiles of schools of public health: implications for public health workforce education and training in Israel
Authors: Neumark Yehuda , Hannink Attal Jordan , Shapiro Naham , MacLeod Fiona , Harrington Janas , Barach Paul , de Nooijer Jascha , Dopelt Keren , Duplaga Mariusz , Leighton Lore , Levine Hagai , Mor Zohar , Otok Robert , Paillard-Borg Stephanie , Tulchinsky Ted , Zelber-Sagi Shira , Malowany Maureen
Published: August 2024

Aim

Competency frameworks are essential for analyzing capabilities of Schools of Public Health to adequately prepare public health (PH) professionals to address contemporary challenges. This study maps the competency profiles of PH training programs in Israel using a novel curriculum mapping tool.

Methods

This study assessed all five Israeli Health Education Institutions (HEIs) offering MPH or Bachelors in Public Health (BPH) degrees across 57 competencies in six domains to determine the extent to which competencies were addressed in the curriculum. The competencies list was based on the Association of Schools of Public Health in the European Region (ASPHER) List of Core Competences for the Public Health Professional, adapted for Israeli HEIs.

Results

The core curricula in the four MPH programs addressed 45–84% of all competencies. The BPH program addressed 79% of competencies. In MPH programs, the core curricula addressed most or all competencies in the Methods and the Socioeconomic Determinants of Health domains. Competencies in the domains of Environmental Determinants of Health, Health Policy, Economics & Organization, and Health Promotion and Prevention were less comprehensively addressed in most core curricula. Students’ opportunities to broaden their exposure to competencies outside the core curricula were context dependent.

Discussion

The curriculum competencies mapping tool that was developed served to assess both strengths and shortcomings in PH education in Israel. The findings demonstrate a highly variable array of PH curriculum models in Israeli HEIs, as well as overall shortcomings in the Environmental, Health Policy Economics and Organization, and Health Promotion and Prevention domains. This analysis has already led to reassessment of the curriculum, and will continue to guide the next steps to increase the harmonization of PH training curricula and to better meet PH challenges in Israel.
Translation, cross-cultural adaptation and validation of the universal Welch Emotional Connection Screen using primary and bilingual Spanish-speaking coders of videotaped mother-child interactions
Authors: Hane Amie A. , Ludwig Robert J. , Martinez Amy G. , Masese Cynthia , Vanhatalo Ulla , Goddard Cliff , Jaffe Marc E. , Myers Michael M. , Welch Martha G.
Published: August 2024

Introduction

Using clear explicit translatable language, we translated the Welch Emotional Connection Screen into a new universal language instrument, the English uWECS. In this study, we had two aims: Aim 1 was to establish concurrent validity of the uWECS by comparing scores coded by primary Spanish-speaking coders using the Spanish translation of the uWECS to scores coded by bilingual, secondary Spanish-speaking coders using the oWECS. Aim 2 was to establish the criterion-related validity in terms of oWECS and uWECS performance in tracking change in autonomic emotional connection (AEC) during the course of an intervention among preschool aged children.

Methods

We created a library of 52 five-minute Spanish-speaking mother-child videos that were collected during a randomized controlled trial of Mother-Child Emotional Preparation intervention (MCEP). The videos were collected at two time points, at enrollment and at a 6-month follow-up. The subsample of Primary Spanish-Speaking dyads from the MCEP study were coded by two independent teams of coders. We trained primary English-speaking (bilingual Spanish) coders on the oWECS, using the original training program. A different team of primary Spanish-speaking coders coded the same cases using the novel uWECS guide and trained briefly for reliability with the Spanish uWECS translation materials.

Results

We found that the Spanish oWECS and Spanish uWECS ratings from the baseline and 6-month follow-up observations were robustly correlated, with intraclass correlations ranging from .81 to .84 and all p-values<.001, thus demonstrating sound concurrent validity for the uWECS. The oWECS and uWECS scores also achieved parallel results when evaluating the efficacy of the MCEP for primary Spanish-speaking dyads. Both the AEC scores of the oWECS [F(1, 27) = 4.31, p < .05] and the scores of the uWECS [F(1,27) = 4.06, p < .05] similarly demonstrated significant change post intervention, thus demonstrating sound criterion-related validity of the uWECS.

Discussion

These findings demonstrate that the uWECS can be used to measure parent/child AEC in linguistically diverse populations and cultures.
Mirabegron 50 mg once daily, long-term treatment maximizes benefit in middle-aged and older people with overactive bladder syndrome: a systematic review and meta-analysis of nine phase II/III, randomized, double-blind, parallel-design, placebo-controlled, multicenter, and multinational trials
Authors: Zhang Xiangxiang , Mao Yinhui , Liu Yang , Sun Jilei , Sun Juntao , Pan Chenli , Wang Zhuo , Wei Zhitao , Yang Yong
Published: August 2024

The prevalence and severity of overactive bladder increase with age, and mirabegron is an approved treatment for this condition. This meta-analysis systematically evaluated the efficacy and safety of mirabegron compared with placebo for overactive bladder treatment. We searched PubMed and the Cochrane Library (30 October 2023) for relevant articles (source: MEDLINE, EMBASE, ClinicalTrials.gov, ICTRP, CINAHL). We included randomized controlled trials involving adults with overactive bladder syndrome that compared mirabegron with placebo treatment. Data were analyzed according to the Cochrane Handbook for Systematic Reviews of Interventions [Review Manager (computer program) Version 5.4]. Nine parallel-group trials (10 articles) were included. The evaluation included a total of 8,527 adults, including 6,445 women and 2,082 men, of whom 5,726 were White, 2,462 were Asian, and 161 were Black. The mean age of the participants ranged from 53.4 to 60.3 years. This evaluation involved three specifications of mirabegron: 25 mg, 50 mg, and 100 mg. In all trials, patients were enrolled in a 12-week double-blind treatment period, and the dose was once daily. The review of trials found that on average, people taking mirabegron had about 13 ml more volume voided per micturition, five fewer micturitions, and four fewer incontinence episodes every week, with moderate improvements in quality of life. About one in five people taking the drug reported TRAEs. Mirabegron treatment is well tolerated, with the risk of adverse events similar to that of a placebo. For best results, a dose of 50 mg once daily is recommended for long-term use. It is unclear whether any benefits are sustained after treatment discontinuation.

Systematic Review Registration: https://www.crd.york.ac.uk/prospero/, PROSPERO (CRD42023430737).
Muscle growth differences in Lijiang pigs revealed by ATAC-seq multi-omics
Authors: Lan Yi , Yan Dawei , Li Xinpeng , Zhou Chunlu , Bai Ying , Dong Xinxing
Published: August 2024

As one of the largest tissues in the animal body, skeletal muscle plays a pivotal role in the production and quality of pork. Consequently, it is of paramount importance to investigate the growth and developmental processes of skeletal muscle. Lijiang pigs, which naturally have two subtypes, fast-growing and slow-growing, provide an ideal model for such studies by eliminating breed-related influences. In this study, we selected three fast-growing and three slow-growing 6-month-old Lijiang pigs as subjects. We utilized assay for transposase-accessible chromatin with sequencing (ATAC-seq) combined with genomics, RNA sequencing, and proteomics to screen for differentially expressed genes and transcription factors linked to increased longissimus dorsi muscle volume in Lijiang pigs. We identified 126 genes through ATAC-seq, including PPARA, TNRC6B, NEDD1, and FKBP5, that exhibited differential expression patterns during muscle growth. Additionally, we identified 59 transcription factors, including Foxh1, JunB, Mef2 family members (Mef2a/b/c/d), NeuroD1, and TEAD4. By examining open chromatin regions (OCRs) with significant genetic differentiation, genes such as SAV1, CACNA1H, PRKCG, and FGFR4 were found. Integrating ATAC-seq with transcriptomics and transcriptomics with proteomics, we identified differences in open chromatin regions, transcription, and protein levels of FKBP5 and SCARB2 genes in fast-growing and slow-growing Lijiang pigs. Utilizing multi-omics analysis with R packages, we jointed ATAC-seq, transcriptome, and proteome datasets, identifying enriched pathways related to glycogen metabolism and skeletal muscle cell differentiation. We pinpointed genes such as MYF6 and HABP2 that exhibit strong correlations across these diverse data types. This study provides a multi-faceted understanding of the molecular mechanisms that lead to differences in pig muscle fiber growth.
Diagnostic challenges in patients with Castleman disease, a single center experience from Hungary
Authors: Brúgós Boglárka , Simon Zsófia , Méhes Gábor , Illés Árpád , Pfliegler György
Published: August 2024

Castleman disease is a rare and atypical lymphoproliferative disorder characterized by diverse clinical manifestations. It has both unicentric and multicentric forms, the latter with further subdivisions, i.e., human herpesvirus 8-associated and idiopathic forms. The diagnosis of Castleman disease is often delayed, as it is rare, and because it shares clinical features with different autoimmune, inflammatory, and malignant lymphoproliferative disorders. The first-line treatment in unicentric form is mainly surgical, while in idiopathic Castleman disease, anti-interleukin-6 treatment is the therapy of choice. In virus-associated diseases, antiretroviral therapy and rituximab are recommended. In Hungary, only a few cases of Castleman disease have been published. This report presents our two decades of experience in the challenging diagnosis and management of this rare disorder, most properly underdiagnosed in Hungary. We provide insights into seven unicentric and five idiopathic multicentric Castleman disease cases, the latter ones especially highlighting the diagnostic and therapeutic challenges due to the variable and unique clinical features both of patients and diseases, e.g., bronchiolitis obliterans, stage IV diabetic renal failure, anti-HBc positivity, siltuximab treatment period, respectively.
Target Tracking and Circumnavigation Control for Multi-Unmanned Aerial Vehicle Systems Using Bearing Measurements
Authors: Zhou Zican , Hu Jiangping , Chen Bo , Shen Xixi , Meng Bin
Published: August 2024

This paper addresses the problem of target tracking and circumnavigation control for a bearing-only multi-Unmanned Aerial Vehicle (UAV) system. First, using the bearing measurements, an adaptive algorithm in the form of a Proportional Integral (PI) controller is developed to estimate the target state. Subsequently, a distributed circumnavigation control protocol is established to evenly encircle the target. Then, we use the local information from each UAV in the network to calculate the relative position of the target, and further enhance the accuracy of estimation and circumnavigation algorithms by employing a Kalman filter. Finally, numerical simulation experiments are conducted to validate the effectiveness of the proposed tracking control algorithm.
Observer-Based Finite-Time Prescribed Performance Sliding Mode Control of Dual-Motor Joints-Driven Robotic Manipulators with Uncertainties and Disturbances
Authors: Xu Jiqian , Fang Lijin , Wang Huaizhen , Zhao Qiankun , Wan Yingcai , Gao Yue
Published: August 2024

Considering system uncertainties (e.g., gear backlash, unmodeled dynamics, nonlinear friction and parameters perturbation) coupling disturbances weaken the motion performance of robotic systems, an observer-based finite-time prescribed performance sliding mode control with faster reaching law is proposed for robotic manipulators equipped with dual-motor joints (DMJs). In the case where the backlash information is completely unknown, the backlash is maximally eliminated using a simple but efficient dual-motor adaptive anti-backlash strategy. Thus, the design of position tracking controllers for DMJs can be simplified. Then, to deal with the influence of disturbances and residual uncertainties (excluding backlash), a novel finite-time adaptive sliding mode disturbance observer (ASMDO) is proposed to practically estimate the lumped uncertainties where their upper bounds are assumed to be unknown. Finally, a finite-time composite fast non-singular terminal sliding mode (TSM) controller, integrated with the prescribed performance principle, is proposed in this paper. To enhance the convergence rate, a novel TSM-type reaching law has been developed. The controller ensures that the tracking error is not only stabilized within a finite-time convergence rate but also adheres to a predefined maximum transient-steady-state error. The proposed scheme is implemented through simulation and experimental results, demonstrating its superior performance.
Modeling and Control of Reconfigurable Quadrotors Based on Model Reference Adaptive Control
Authors: Liu Zhiping , Chen Guoshao , Xu Shuping
Published: August 2024

To expand the application prospects of quadrotors in challenging scenes such as those with dense obstacles and narrow corridors, task-driven reconfigurable quadrotors are highly desirable. Aiming to address hazard missions, in this paper, translational reconfigurable quadrotors and rotational reconfigurable quadrotors are proposed with their assumptions and mathematical models. Related motion control laws were designed using model reference adaptive control (MRAC) theory based on Lyapunov stability theory, whose validity was demonstrated by sufficient numerical simulations. The simulation results verify the feasibility of the proposed control laws and reveal the important effect of time delay on the stability of the motion control system. Additionally, the dependence of motion control’s stability on the time constant of reference system was discussed.
Implementation of a 6U CubeSat Electrical Power System Digital Twin
Authors: Casado Pablo , Torres Cristian , Blanes José M. , Garrigós Ausiàs , Marroquí David
Published: August 2024

This paper presents the design of a digital twin for a 6U CubeSat electrical power system, including the solar arrays, solar array regulators, battery, power distribution unit, and load subsystems. The digital twin is validated by comparing its real-time outputs with those of the physical system. Experimental tests confirm its feasibility, showing that the digital twin’s real-time outputs closely match those of the physical system. Additionally, the digital twin can be used for control-hardware-in-the-loop and power-hardware-in-the-loop tests, allowing the real-time integration of simulated subsystems with hardware. This capability facilitates testing of new subsystems and optimization during the project’s development phases. Additionally, to demonstrate the advanced capabilities of this model, the digital twin is used to simulate the CubeSat electrical power system behavior in real time throughout a complete orbital cycle in low Earth orbit conditions. This simulation provides valuable insights into the CubeSat operation by capturing the transient and steady-state responses of the EPS components under real orbital conditions. The results obtained indicate that the digital twin significantly enhances the testing and optimization process of new subsystems during the development phases of the project. Moreover, the capabilities of the digital twin can be further augmented by incorporating real-time telemetry data from the CubeSat, resulting in a highly accurate replication of the satellite’s in-orbit behavior. This approach is crucial for identifying and diagnosing failures or malfunctions in the electrical power system, ensuring the robust and reliable operation of the CubeSat.
Mental Workload as a Predictor of ATCO’s Performance: Lessons Learnt from ATM Task-Related Experiments
Authors: Muñoz-de-Escalona Enrique , Leva Maria Chiara , Cañas José Juan
Published: August 2024

Air Traffic Controllers’ (ATCos) mental workload is likely to remain the specific greatest functional limitation on the capacity of the Air Traffic Management (ATM) system. Developing computational models to monitor mental workload and task complexity is essential for enabling ATCOs and ATM systems to adapt to varying task demands. Most methodologies have computed task complexity based on basic parameters such as air-traffic density; however, literature research has shown that it also depends on many other factors. In this paper, we present a study in which we explored the possibility of predicting task complexity and performance through mental workload measurements of participants performing an ATM task in an air-traffic control simulator. Our findings suggest that mental workload measurements better predict poor performance and high task complexity peaks than other established factors. This underscores their potential for research into how different ATM factors affect task complexity. Understanding the role and the weight of these factors in the overall task complexity confronted by ATCos constitutes one of the biggest challenges currently faced by the ATM sphere and would significantly contribute to the safety of our sky.
Mars Exploration: Research on Goal-Driven Hierarchical DQN Autonomous Scene Exploration Algorithm
Authors: Zhou Zhiguo , Chen Ying , Yu Jiabao , Zu Bowen , Wang Qian , Zhou Xuehua , Duan Junwei
Published: August 2024

In the non-deterministic, large-scale navigation environment under the Mars exploration mission, there is a large space for action and many environmental states. Traditional reinforcement learning algorithms that can only obtain rewards at target points and obstacles will encounter the problems of reward sparsity and dimension explosion, making the training speed too slow or even impossible. This work proposes a deep layered learning algorithm based on the goal-driven layered deep Q-network (GDH-DQN), which is more suitable for mobile robots to explore, navigate, and avoid obstacles without a map. The algorithm model is designed in two layers. The lower layer provides behavioral strategies to achieve short-term goals, and the upper layer provides selection strategies for multiple short-term goals. Use known position nodes as short-term goals to guide the mobile robot forward and achieve long-term obstacle avoidance goals. Hierarchical execution not only simplifies tasks but also effectively solves the problems of reward sparsity and dimensionality explosion. In addition, each layer of the algorithm integrates a Hindsight Experience Replay mechanism to improve performance, make full use of the goal-driven function of the node, and effectively avoid the possibility of misleading the agent by complex processes and reward function design blind spots. The agent adjusts the number of model layers according to the number of short-term goals, further improving the efficiency and adaptability of the algorithm. Experimental results show that, compared with the hierarchical DQN method, the navigation success rate of the GDH-DQN algorithm is significantly improved, and it is more suitable for unknown scenarios such as Mars exploration.
Validation of Experimental Data for the Application of the Magnesium Alloy “Elektron 43”
Authors: Guida Michele
Published: August 2024

The behaviour of a structural component, such as the spreader installed on an aeroplane passenger seat made of the magnesium alloy Elektron® 43, is evaluated under a variety of load conditions. The purpose of this research project is to considerably reduce weight by employing the new alloy while keeping the strength and ductility necessary to meet the dynamic standards for both the 16 g forward and 14 g downward tests. A comprehensive campaign of static and dynamic testing on coupons was conducted to characterise the mechanical behaviour of the E43 magnesium alloy, from quasi-static to dynamic loading, and across a wide range of deformation rates. The elastic–plastic and strain rate sensitive material model of E43 is then calibrated using an FEA approach and LS-DYNA software, utilising stress–strain curves and properties determined from standardised experimental tensile and compression trials at varied strain rates. Finally, this material model was used to perform a finite element structural study of a major component of an aeroplane seat built using Elektron® 43 under typical in-flight stresses.
Special Issue “Gust Influences on Aerospace”
Authors: Wu Zhenlong , Hölling Michael
Published: August 2024

An important prerequisite for the design, assessment, and certification of aircraft, their propulsion systems, and associated control systems is a quantitative specification of the environment in which the aircraft are intended to operate [...]
An Endurance Equation for Hybrid-Electric Aircraft
Authors: Batra Aman , Raute Reiko , Camilleri Robert
Published: August 2024

This paper introduces a new endurance equation for a hybrid-electric aircraft. This research follows the derivation of a range equation for a hybrid-electric aircraft case using constant power split that was carried out by authors in their earlier work. Thus, the derivation of the endurance equation maintains the use of efficiency-based degree of hybridization (φ) used in the earlier research. For coherence, the paper also uses the same case study to assess endurance values over a range of battery energy density values and degree of hybridization (φ) values. Results show that any aircraft design has an Energy Density Threshold (EDT) value, before which the endurance of the aircraft reduces with an increase in the degree of hybridization values. Conversely, once EDT is exceeded, the endurance of the aircraft enhances with the increase in the degree of hybridization values. The EDT values are specific to the aircraft type, its specifications and key design parameters.