bluuebunny/crossref_metadata_2025 huggingface dataset:
Cognitive Function, Healthy Lifestyle, and All-Cause Mortality among Chinese Older Adults: A Longitudinal Prospective Study
Authors: Li Huiwen , Zheng Yi , Li Qi , Wang Mengying
Published: April 2024

Background: Both cognitive decline and unhealthy lifestyles have been linked to an elevated risk of mortality in older people. We aimed to investigate whether a healthy lifestyle might modify the association between cognitive function and all-cause mortality in Chinese older populations. Methods: The final analysis included 5124 individuals free of dementia, selected from the Chinese Longitudinal Healthy Longevity Survey from 2011 to 2018. Cognitive function was assessed in 2011 using the Mini-Mental State Examination (MMSE). A lifestyle score was calculated based on five lifestyle factors, including smoking, alcohol consumption, physical activity, diet, and body mass index. Cox proportional hazards models were performed to evaluate the association between baseline cognitive function and the risk of all-cause mortality, with an interaction term of cognitive function and lifestyle score being added to the models. Results: The average age of participants was 81.87 years old at baseline. During a median follow-up of 6.4 years, 1461 deaths were documented. Both higher cognitive function (HR: 0.96; 95% CI: 0.96–0.97) and a healthier lifestyle (HR: 0.92; 95% CI: 0.87–0.97) were significantly associated with a reduced risk of mortality. We found that lifestyle significantly modified the association of cognitive function with mortality (p for interaction = 0.004). The inverse relation between cognitive function and mortality was found to be more pronounced among participants with a healthier lifestyle. Of note, among the lifestyle scores component, diet showed a significant interaction with mortality (p for interaction = 0.003), and the protective HR of the all-cause mortality associated with higher MMSE scores was more prominent among participants with healthy diets compared with unhealthy diets. Conclusions: Our study indicates that cognitive decline is associated with a higher risk of mortality, and such associations are attenuated by maintaining a healthy lifestyle, with a particular emphasis on healthy diet.
Effects of sn-2 Palmitic Triacylglycerols and the Ratio of OPL to OPO in Human Milk Fat Substitute on Metabolic Regulation in Sprague-Dawley Rats
Authors: Zhu Lin , Fang Shuaizhen , Zhang Yaqiong , Sun Xiangjun , Yang Puyu , Lu Weiying , Yu Liangli
Published: April 2024

In this study, the influence of total sn-2 palmitic triacylglycerols (TAGs) and ratio of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) to 1,3-dioleoyl-2-palmitoylglycerol (OPO) in human milk fat substitute (HMFS) on the metabolic changes were investigated in Sprague–Dawley rats. Metabolomics and lipidomics profiling analysis indicated that increasing the total sn-2 palmitic TAGs and OPL to OPO ratio in HMFS could significantly influence glycine, serine and threonine metabolism, glycerophospholipid metabolism, glycerolipid metabolism, sphingolipid metabolism, bile acid biosynthesis, and taurine and hypotaurine metabolism pathways in rats after 4 weeks of feeding, which were mainly related to lipid, bile acid and energy metabolism. Meanwhile, the up-regulation of taurine, L-tryptophan, and L-cysteine, and down-regulations of lysoPC (18:0) and hypoxanthine would contribute to the reduction in inflammatory response and oxidative stress, and improvement of immunity function in rats. In addition, analysis of targeted biochemical factors also revealed that HMFS-fed rats had significantly increased levels of anti-inflammatory factor (IL-4), immunoglobulin A (IgA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-px), and decreased levels of pro-inflammatory factors (IL-6 and TNF-α) and malondialdehyde (MDA), compared with those of the control fat-fed rats. Collectively, these observations present new in vivo nutritional evidence for the metabolic regulatory effects of the TAG structure and composition of human milk fat substitutes on the host.
Estimation of the Impact of Abdominal Adipose Tissue (Subcutaneous and Visceral) on the Occurrence of Carbohydrate and Lipid Metabolism Disorders in Patients with Obesity—A Pilot Study
Authors: Witczak-Sawczuk Katarzyna , Ostrowska Lucyna , Cwalina Urszula , Leszczyńska Joanna , Jastrzębska-Mierzyńska Marta , Hładuński Marcin Krzysztof
Published: April 2024

Obesity represents a significant global public health concern. The excessive accumulation of abdominal adipose tissue is often implicated in the development of metabolic complications associated with obesity. Our study aimed to investigate the impact of particular deposits of abdominal adipose tissue on the occurrence of carbohydrate and lipid metabolism complications. We established cut-off points for visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), and the VAT/SAT ratio at which selected metabolic complications of obesity-related diseases (disorders of carbohydrate and/or lipid metabolism) occur. We conducted an observational study involving 91 subjects with first- and second-degree obesity, accounting for gender differences. Anthropometric measurements were taken, body composition analysis (BIA) was conducted, and biochemical determinations were made. Our findings suggest that commonly used parameters for assessing early metabolic risk, such as BMI or waist circumference, may overlook the significant factor of body fat distribution, as well as gender differences. Both visceral and subcutaneous adipose tissue were found to be important in estimating metabolic risk. We identified the cut-off points in women in terms of their elevated fasting glucose levels and the presence of insulin resistance (HOMA-IR: homeostasis model assessment of insulin resistance) based on SAT, VAT, and the VAT/SAT ratio. In men, cut-off points were determined for the presence of insulin resistance (HOMA-IR) based on VAT and the VAT/SAT ratio. However, the results regarding lipid disorders were inconclusive, necessitating further investigation of a larger population.
Echinacea purpurea Polysaccharide Ameliorates Dextran Sulfate Sodium-Induced Colitis by Restoring the Intestinal Microbiota and Inhibiting the TLR4-NF-κB Axis
Authors: Wei Fan-Hao , Xie Wen-Yin , Zhao Pei-Sen , Gao Wei , Gao Fei
Published: April 2024

Ulcerative colitis (UC) is a chronic intestinal ailment which cannot be completely cured. The occurrence of UC has been on the rise in recent years, which is highly detrimental to patients. The effectiveness of conventional drug treatment is limited. The long-term usage of these agents can lead to substantial adverse effects. Therefore, the development of a safe and efficient dietary supplement is important for the prevention of UC. Echinacea purpurea polysaccharide (EPP) is one of the main bioactive substances in Echinacea purpurea. EPP has many favorable effects, such as antioxidative, anti-inflammatory, and antitumor effects. However, whether EPP can prevent or alleviate UC is still unclear. This study aims to analyze the effect and mechanism of EPP on UC in mice using a 3% dextran sulfate sodium (DSS)-induced UC model. The results showed that dietary supplementation with 200 mg/kg EPP significantly alleviated the shortening of colon length, weight loss, and histopathological damage in DSS-induced colitis mice. Mechanistically, EPP significantly inhibits the activation of the TLR4/NF-κB pathway and preserves the intestinal mechanical barrier integrity by enhancing the expression of claudin-1, ZO-1, and occludin and reducing the loss of goblet cells. Additionally, 16S rRNA sequencing revealed that EPP intervention reduced the abundance of Bacteroides, Escherichia–Shigella, and Klebsiella; the abundance of Lactobacillus increased. The results of nontargeted metabonomics showed that EPP reshaped metabolism. In this study, we clarified the effect of EPP on UC, revealed the potential function of EPP, and supported the use of polysaccharide dietary supplements for UC prevention.
Seasonal Variation in Vitamin D Status Does Not Interfere with Improvements in Aerobic and Muscular Endurance in Conscripts during Basic Military Training
Authors: Timpmann Saima , Rips Leho , Olveti Indrek , Mooses Martin , Mölder Hanno , Varblane Ahti , Lille Hele-Reet , Gapeyeva Helena , Ööpik Vahur
Published: April 2024

Considering a lack of respective data, the primary objective of this study was to assess whether seasonal variation in vitamin D status (D-status) affects the extent of improvement in physical performance (PP) in conscripts during basic military training (BMT). D-status, PP and several blood parameters were measured repeatedly in conscripts whose 10-week BMT started in July (cohort S-C; n = 96) or in October (cohort A-C; n = 107). D-status during BMT was higher in S-C compared to A-C (overall serum 25(OH)D 61.4 ± 16.1 and 48.5 ± 20.7 nmol/L, respectively; p < 0.0001). Significant (p < 0.05) improvements in both aerobic and muscular endurance occurred in both cohorts during BMT. Pooled data of the two cohorts revealed a highly reliable (p = 0.000) but weak (R2 = 0.038–0.162) positive association between D-status and PP measures both at the beginning and end of BMT. However, further analysis showed that such a relationship occurred only in conscripts with insufficient or deficient D-status, but not in their vitamin D-sufficient companions. Significant (p < 0.05) increases in serum testosterone-to-cortisol ratio and decreases in ferritin levels occurred during BMT. In conclusion, a positive association exists between D-status and PP measures, but seasonal variation in D-status does not influence the extent of improvement in PP in conscripts during BMT.
Therapeutic Applications of Aggregatibacter actinomycetemcomitans Leukotoxin
Authors: Kachlany Scott C. , Vega Brian A.
Published: April 2024

Aggregatibacter actinomycetemcomitans is a Gram-negative oral bacterium that has been primarily studied for its role in causing periodontal disease. The bacterium has also been implicated in several systemic diseases such as endocarditis and soft tissue abscesses. Leukotoxin (LtxA) is perhaps the best studied protein virulence factor from A. actinomycetemcomitans. The protein can rapidly destroy white blood cells (WBCs), helping the bacterium to subvert the host immune system. The functional receptor for LtxA is lymphocyte function associated antigen-1 (LFA-1), which is expressed exclusively on the surfaces of WBCs. Bacterial expression and secretion of the protein are highly regulated and controlled by a number of genetic and environmental factors. The mechanism of LtxA action on WBCs varies depending on the type of cell that is being killed, and the protein has been shown to activate numerous cell death pathways in susceptible cells. In addition to serving as an important virulence factor for the bacterium, because of its exquisite specificity and rapid activity, LtxA is also being investigated as a therapeutic agent that may be used to treat diseases such as hematological malignancies and autoimmune/inflammatory diseases. It is our hope that this review will inspire an increased intensity of research related to LtxA and its effect on Aggressive Periodontitis, the disease that led to its initial discovery.
Gastric Cancer, Immunotherapy, and Nutrition: The Role of Microbiota
Authors: Raoul Pauline , De Gaetano Valeria , Sciaraffia Gianmario , Ormea Ginevra , Cintoni Marco , Pozzo Carmelo , Strippoli Antonia , Gasbarrini Antonio , Mele Maria Cristina , Rinninella Emanuele
Published: April 2024

Immune checkpoint inhibitors (ICI) have revolutionized the treatment of gastric cancer (GC), which still represents the third leading cause of cancer-related death in Western countries. However, ICI treatment outcomes vary between individuals and need to be optimized. Recent studies have shown that gut microbiota could represent a key influencer of immunotherapy responses. At the same time, the nutritional status and diet of GC patients are also predictive of immunotherapy treatment response and survival outcomes. The objective of this narrative review is to gather recent findings about the complex relationships between the oral, gastric, and gut bacterial communities, dietary factors/nutritional parameters, and immunotherapy responses. Perigastric/gut microbiota compositions/functions and their metabolites could be predictive of response to immunotherapy in GC patients and even overall survival. At the same time, the strong influence of diet on the composition of the microbiota could have consequences on immunotherapy responses through the impact of muscle mass in GC patients during immunotherapy. Future studies are needed to define more precisely the dietary factors, such as adequate daily intake of prebiotics, that could counteract the dysbiosis of the GC microbiota and the impaired nutritional status, improving the clinical outcomes of GC patients during immunotherapy.
In Silico Prediction of New Inhibitors for Kirsten Rat Sarcoma G12D Cancer Drug Target Using Machine Learning-Based Virtual Screening, Molecular Docking, and Molecular Dynamic Simulation Approaches
Authors: Ajmal Amar , Danial Muhammad , Zulfat Maryam , Numan Muhammad , Zakir Sidra , Hayat Chandni , Alabbosh Khulood Fahad , Zaki Magdi E. A. , Ali Arif , Wei Dongqing
Published: April 2024

Single-point mutations in the Kirsten rat sarcoma (KRAS) viral proto-oncogene are the most common cause of human cancer. In humans, oncogenic KRAS mutations are responsible for about 30% of lung, pancreatic, and colon cancers. One of the predominant mutant KRAS G12D variants is responsible for pancreatic cancer and is an attractive drug target. At the time of writing, no Food and Drug Administration (FDA) approved drugs are available for the KRAS G12D mutant. So, there is a need to develop an effective drug for KRAS G12D. The process of finding new drugs is expensive and time-consuming. On the other hand, in silico drug designing methodologies are cost-effective and less time-consuming. Herein, we employed machine learning algorithms such as K-nearest neighbor (KNN), support vector machine (SVM), and random forest (RF) for the identification of new inhibitors against the KRAS G12D mutant. A total of 82 hits were predicted as active against the KRAS G12D mutant. The active hits were docked into the active site of the KRAS G12D mutant. Furthermore, to evaluate the stability of the compounds with a good docking score, the top two complexes and the standard complex (MRTX-1133) were subjected to 200 ns MD simulation. The top two hits revealed high stability as compared to the standard compound. The binding energy of the top two hits was good as compared to the standard compound. Our identified hits have the potential to inhibit the KRAS G12D mutation and can help combat cancer. To the best of our knowledge, this is the first study in which machine-learning-based virtual screening, molecular docking, and molecular dynamics simulation were carried out for the identification of new promising inhibitors for the KRAS G12D mutant.
Comparative Investigation of pH–Dependent Availability of Pancreatic Enzyme Preparations In Vitro
Authors: Todd Amy , Bennett-Huntley Emma , Rosendahl Jonas , Schnekenburger Jürgen , Uhl Waldemar
Published: April 2024

This study aimed to compare different pancreatic enzyme preparations (PEPs) available in Germany regarding particle geometry and size, and to evaluate enzyme activity under physiologically relevant conditions in vitro. Pancreatic endocrine insufficiency is characterized by deficiency of pancreatic enzymes resulting in maldigestion. It is orally treated by pancreatic enzyme replacement therapy. The formulations differ in their physical properties and enzyme release behavior, potentially resulting in inconsistent dosages and poor interchangeability of products. A total of 25 products were analyzed for particle size and number of particles per capsule. Enzyme activities of lipase, amylase, and protease were measured by digestion of olive oil emulsion, starch, and casein, respectively. To analyze enzyme release, gastric environments were simulated by incubating PEPs at pH 1, 4, or 5. Duodenal conditions were simulated by subsequent incubation at pH 6. Regarding physical properties and enzyme release kinetics, considerable differences between different PEPs were found. Furthermore, compared to the label claim, excess lipase activity was observed for most products, reaching up to 148%. These in vitro results suggest poor interchangeability of PEPs, potentially explained by physical and release characteristics. Physicians and patients should be aware of the potential gap between label claims and the real-life performance of different PEPs.
Plant Protease Inhibitors as Emerging Antimicrobial Peptide Agents: A Comprehensive Review
Authors: Parisi Mónica G. , Ozón Brenda , Vera González Sofía M. , García-Pardo Javier , Obregón Walter David
Published: April 2024

Antimicrobial peptides (AMPs) are important mediator molecules of the innate defense mechanisms in a wide range of living organisms, including bacteria, mammals, and plants. Among them, peptide protease inhibitors (PPIs) from plants play a central role in their defense mechanisms by directly attacking pathogens or by modulating the plant’s defense response. The growing prevalence of microbial resistance to currently available antibiotics has intensified the interest concerning these molecules as novel antimicrobial agents. In this scenario, PPIs isolated from a variety of plants have shown potential in inhibiting the growth of pathogenic bacteria, protozoans, and fungal strains, either by interfering with essential biochemical or physiological processes or by altering the permeability of biological membranes of invading organisms. Moreover, these molecules are active inhibitors of a range of proteases, including aspartic, serine, and cysteine types, with some showing particular efficacy as trypsin and chymotrypsin inhibitors. In this review, we provide a comprehensive analysis of the potential of plant-derived PPIs as novel antimicrobial molecules, highlighting their broad-spectrum antimicrobial efficacy, specificity, and minimal toxicity. These natural compounds exhibit diverse mechanisms of action and often multifunctionality, positioning them as promising molecular scaffolds for developing new therapeutic antibacterial agents.
Point Prevalence Survey of Acute Hospital Patients with Difficulty Swallowing Solid Oral Dose Forms
Authors: Harnett Anne , Byrne Stephen , O’Connor Jennifer , Burke Eimear , South Laura , Lyons Declan , Sahm Laura J.
Published: April 2024

The safe administration of solid oral dose forms in hospital inpatients with swallowing difficulties is challenging. The aim of this study was to establish the prevalence of difficulties in swallowing solid oral dose forms in acute hospital inpatients. A point prevalence study was completed at three time points. The following data were collected: the prevalence of swallowing difficulties, methods used to modify solid oral dose forms to facilitate administration, the appropriateness of the modification, and patient co-morbidities. The prevalence of acute hospital inpatients with swallowing difficulties was an average of 15.4% with a 95% CI [13.4, 17.6] across the three studies. On average, 9.6% of patients with swallowing difficulties had no enteral feeding tube in situ, with 6.0% of these patients receiving at least one modified medicine. The most common method of solid oral dose form modification was crushing, with an administration error rate of approximately 14.4%. The most common co-morbid condition in these patients was hypertension, with dysphagia appearing on the problem list of two (5.5%) acute hospital inpatients with swallowing difficulties. Inappropriate modifications to solid oral dose forms to facilitate administration can result in patient harm. A proactive approach, such as the use of a screening tool to identify acute hospital inpatients with swallowing difficulties, is required, to mitigate the risk of inappropriate modifications to medicines to overcome swallowing difficulties.
Taste-Masked Pellets of Warfarin Sodium: Formulation towards the Dose Personalisation
Authors: Kovalenko Lakija , Kukuls Kirils , Berga Marta , Mohylyuk Valentyn
Published: April 2024

The bitter drug, warfarin, has a narrow therapeutic index (NTI) and is used in paediatrics and geriatrics. The aim of this feasibility study was to formulate the taste-masked warfarin-containing pellets to be applicable for dose personalisation and to improve patient compliance, as well as to investigate the effect of the core type (PharSQ® Spheres M, CELPHERE™ CP-507, and NaCl) on the warfarin release from the Kollicoat® Smartseal taste-masking-coated pellets. The cores were successfully drug-loaded and coated in a fluid-bed coater with a Wurster insert. An increase in particle size and particle size distribution was observed by optical microscopy. In saliva-simulated pH, at the Kollicoat® Smartseal level of 2 mg/cm2, none of the pellets demonstrated drug release, confirming their efficient taste-masking. However, in a stomach-simulated pH, a faster drug release was observed from PharSQ® Spheres M- and CELPHERE™ CP-507-coated pellets in comparison with NaCl cores. Additional experiments allowed us to explain the slower drug release from NaCl-containing pellets because of the salting-out effect. Despite the successful taste masking, the drug release from pellets was relatively slow (not more than 91% per 60 min), allowing for further formulation improvements.
In Vitro Stability and Pharmacokinetic Study of Pedunculoside and Its Beta-CD Polymer Inclusion Complex
Authors: Wu Liang , Li Danfeng , Wang Peijing , Dong Linling , Zhang Wang , Xu Jianjun , Jin Xiaoliang
Published: April 2024

Pedunculoside, a triterpene saponin derived from various Ilex species, holds potential as a treatment for cardiovascular diseases. However, its clinical application is hindered by poor bioavailability, rapid elimination, and extensive intestinal metabolism to rotundic acid. To address these issues, a water-soluble inclusion complex of pedunculoside, namely, the beta-CD polymer inclusion complex of pedunculoside (pedunculoside–βCDP), was prepared in this study, and a comparative in vitro stability and pharmacokinetic behavior study was performed between pedunculoside and pedunculoside–βCDP. Both pedunculoside and pedunculoside–βCDP exhibited the highest stability in simulated gastric fluid and simulated intestinal fluid but were readily metabolized when co-incubated with Bifidobacterium adolescentis and Bifidobacterium breve. An LC-MS/MS analytical method for the simultaneous determination of pedunculoside and rotundic acid in rat plasma was successfully established, validated, and applied to investigate the pharmacokinetic behavior after rats were intravenously administered with pedunculoside or pedunculoside–βCDP. The results indicated that pedunculoside–βCDP could significantly improve the pharmacokinetic profile of pedunculoside by increasing plasma exposure, retarding elimination, and reducing intestinal metabolism. This study enhances our understanding of pedunculoside–βCDP’s metabolic fate and pharmacokinetic properties and potentially advances its further research, development, and clinical application.
High Fidelity Full-Color Optical Sectioning Structured Illumination Microscopy by Fourier Domain Based Reconstruction
Authors: Dang Shipei , Qian Jia , Ma Wang , Ma Rui , Li Xing , Wang Siying , Bai Chen , Dan Dan , Yao Baoli
Published: April 2024

The natural color of biological specimens plays a crucial role in body protection, signaling, physiological adaptations, etc. Full-color optical sectioning structured illumination microscopy (OS-SIM) color is a promising approach that can reconstruct biological specimens in three-dimension meanwhile maintaining their natural color. Full-color OS-SIM takes the advantages of rapid imaging speed, compatibility with fluorescence and non-fluorescence samples, compact configuration, and low cost. However, the commonly used HSV-RMS reconstruction algorithm for full-color OS-SIM faces two issues to be improved. One is the RMS (root-mean-square) OS reconstruction algorithm is prone to background noise, and the other is the reconstruction is bound in RGB and HSV color spaces, consuming more reconstructing time. In this paper, we propose a full-color Fourier-OS-SIM method that allows for the OS reconstruction using the high-frequency spectrum of the sample and thus is immune to the low-frequency background noise. The full-color Fourier-OS-SIM directly runs in the RGB color space, providing an easy way to restore the color information. Simulation and experiments with various samples (pollen grains and tiny animals) demonstrate that the full-color Fourier-OS-SIM method is superior to the HSV-RMS method regarding background noise suppression. Moreover, benefiting from the background noise suppression merit, the quantitative morphological height map analysis with the full-color Fourier-OS-SIM method is more accurate. The proposed full-color Fourier-OS-SIM method is expected to find broad applications in biological and industrial fields where the 3D morphology and the color information of objects both need to be recovered.
Phenolic Monoterpenes Conversion of Conobea scoparioides Essential Oil by Hydrotalcite Synthesized from Blast-Furnace Slag
Authors: Rebelo Monaliza M. , Andrade Eloisa Helena A. , Corrêa José Augusto M. , Maia José Guilherme S.
Published: April 2024

Conobea scoparioides (Plantaginaceae) is an herbaceous plant known as “pataqueira” that grows wild in seasonally wet areas of the Amazon region. It is used for aromatic baths and anti-protozoan remedies by the Brazilian Amazon native people. The main volatile compounds identified in the essential oil of “Pataqueira” were the phenolic monoterpenes thymol and thymol methyl ether and their precursors, the monoterpene hydrocarbons α-phellandrene and p-cymene. A hydrotalcite synthesized from blast-furnace slag exhibited a 3:2 (Mg/Al) molar ratio, and this layered double hydroxide (LDH) was evaluated as a catalyst in converting the main monoterpenes of the “Pataqueira” oil. This action significantly increased the thymol content, from 41% to 95%, associated with the percentual reduction in other main components, such as thymol methyl ether, α-phellandrene, and p-cymene. The LDH reaction showed a strong tendency towards producing hydroxylated derivatives, and its behavior was similar to the hypothetical plant biosynthetic pathway, which leads to the production of the monoterpenes of “Pataqueira” oil. Thymol and its derivatives are potent antiseptics applied in pharmaceutical and hygienic products as antibacterial, antifungal, and antioxidant properties, among others. The present work reports a natural source with a high thymol content in aromatic plants from the Amazon, with evident economic value.
Appropriate Drought Training Induces Optimal Drought Tolerance by Inducing Stepwise H2O2 Homeostasis in Soybean
Authors: Shen Yuqian , Li Lei , Du Peng , Xing Xinghua , Gu Zhiwei , Yu Zhiming , Tao Yujia , Jiang Haidong
Published: April 2024

Soybean is considered one of the most drought-sensitive crops, and ROS homeostasis can regulate drought tolerance in these plants. Understanding the mechanism of H2O2 homeostasis and its regulatory effect on drought stress is important for improving drought tolerance in soybean. We used different concentrations of polyethylene glycol (PEG) solutions to simulate the progression from weak drought stress (0.2%, 0.5%, and 1% PEG) to strong drought stress (5% PEG). We investigated the responses of the soybean plant phenotype, ROS level, injury severity, antioxidant system, etc., to different weak drought stresses and subsequent strong drought stresses. The results show that drought-treated plants accumulated H2O2 for signaling and exhibited drought tolerance under the following stronger drought stress, among which the 0.5% PEG treatment had the greatest effect. Under the optimal treatment, there was qualitatively describable H2O2 homeostasis, characterized by a consistent increasing amplitude in H2O2 content compared with CK. The H2O2 signal formed under the optimum treatment induced the capacity of the antioxidant system to remove excess H2O2 to form a primary H2O2 homeostasis. The primary H2O2 homeostasis further induced senior H2O2 homeostasis under the following strong drought and maximized the improvement of drought tolerance. These findings might suggest that gradual drought training could result in stepwise H2O2 homeostasis to continuously improve drought tolerance.
Mapping and Omics Integration: Towards Precise Rice Disease Resistance Breeding
Authors: Ontoy John Christian , Ham Jong Hyun
Published: April 2024

Rice (Oryza sativa), as a staple crop feeding a significant portion of the global population, particularly in Asian countries, faces constant threats from various diseases jeopardizing global food security. A precise understanding of disease resistance mechanisms is crucial for developing resilient rice varieties. Traditional genetic mapping methods, such as QTL mapping, provide valuable insights into the genetic basis of diseases. However, the complex nature of rice diseases demands a holistic approach to gain an accurate knowledge of it. Omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, enable a comprehensive analysis of biological molecules, uncovering intricate molecular interactions within the rice plant. The integration of various mapping techniques using multi-omics data has revolutionized our understanding of rice disease resistance. By overlaying genetic maps with high-throughput omics datasets, researchers can pinpoint specific genes, proteins, or metabolites associated with disease resistance. This integration enhances the precision of disease-related biomarkers with a better understanding of their functional roles in disease resistance. The improvement of rice breeding for disease resistance through this integration represents a significant stride in agricultural science because a better understanding of the molecular intricacies and interactions underlying disease resistance architecture leads to a more precise and efficient development of resilient and productive rice varieties. In this review, we explore how the integration of mapping and omics data can result in a transformative impact on rice breeding for enhancing disease resistance.
Advances in Organosulfur-Based Polymers for Drug Delivery Systems
Authors: Islam Fawad , Zeng Qingle
Published: April 2024

Organosulfur-based polymers have unique properties that make them useful for targeted and managed drug delivery, which can improve therapy while reducing side effects. This work aims to provide a brief review of the synthesis strategies, characterization techniques, and packages of organosulfur-based polymers in drug delivery. More importantly, this work discusses the characterization, biocompatibility, controlled release, nanotechnology, and targeted therapeutic aspects of these important structural units. This review provides not only a good comprehension of organosulfur-based polymers but also an insightful discussion of potential future prospectives in research. The discovery of novel organosulfur polymers and innovations is highly expected to be stimulated in order to synthesize polymer prototypes with increased functional accuracy, efficiency, and low cost for many industrial applications.
Multiscale Modeling and Characterization of Graphene Epoxy Nanocomposite
Authors: Ekeowa Collins , Muthu SD Jacob
Published: April 2024

This study aims to characterize graphene epoxy nanocomposite properties using multiscale modeling. Molecular dynamics was used to study the nanocomposite at the nanoscale and finite element analysis at the macroscale to complete the multiscale modeling. The coupling of these two scales was carried out using the Irving–Kirkwood averaging method. First, the functionalization of graphene was carried and 6% grafted graphene was selected based on Young’s modulus and the tensile strength of the grafted graphene sheet. Functionalized graphene with weight fractions of 1.8, 3.7, and 5.6 wt.% were reinforced with epoxy polymer to form a graphene epoxy nanocomposite. The results showed that the graphene with 3.7 wt.% achieved the highest modulus. Subsequently, a functionalized graphene sheet with an epoxy matrix was developed to obtain the interphase properties using the MD modeling technique. The normal and shear forces at the interphase region of the graphene epoxy nanocomposite were investigated using a traction-separation test to analyze the mechanical properties including Young’s modulus and traction forces. The mean stiffness of numerically tested samples with 1.8, 3.7, and 5.6 wt.% graphene and the stiffness obtained from experimental results from the literature were compared. The experimental results are lower than the multiscale model results because the experiments cannot replicate the molecular-scale behavior. However, a similar trend could be observed for the addition of up to 3.7 wt.% graphene. This demonstrated that the graphene with 3.7 wt.% shows improved interphase properties. The macroscale properties of the graphene epoxy nanocomposite models with 1.8 and 3.7 wt.% were comparatively higher.
Mechanical Properties Comparison of Isotropic vs. Anisotropic Hybrid Magnetorheological Elastomer-Fluid
Authors: Ananzeh Hammam M. , Ramli Rahizar , Julai Sabariah , Muthalif Asan G. A.
Published: April 2024

Magnetorheological (MR) materials are smart materials that can change their rheological characteristics when exposed to a magnetic field. Such rheological properties include viscosity and dynamic modulus. MR materials have emerged as one of the most efficient smart materials that can modify mechanical and viscoelastic characteristics. Depending on the medium used, MR materials can be classified into two types: magnetorheological fluids (MRFs) and magnetorheological elastomers (MREs). MREs are classified as isotropic or anisotropic based on CIP distribution inside the elastomer matrix. A unique hybrid material incorporating MRE and MRF is constructed in this work to investigate, compare, and the dynamic properties of isotropic, anisotropic, hybrid isotropic, and hybrid anisotropic MREs under various magnetic fields (0, 104, and 160.2 mT). The created samples are subjected to extensive testing, including static and dynamic evaluations. In the static tests, experiments use a compression linear displacement mode with a fixed maximum gap change of 3 mm. The temperature is maintained at a constant level of 24 °C throughout the 40 s test duration for each test, and the magnetic field is incrementally increased by varying the number of magnets, ranging from 0 to 160.2 mT for dynamic qualities using compression oscillations on a dynamic mechanical analyzer (DMA), including frequency and strain-dependent data. These experiments, carried out using sinusoidal shear movements, include an excitation frequency range of 0.1 Hz to 15 Hz while preserving, with a fixed shear strain of 2%.
High-Temperature Polylactic Acid Proves Reliable and Safe for Manufacturing 3D-Printed Patient-Specific Instruments in Pediatric Orthopedics—Results from over 80 Personalized Devices Employed in 47 Surgeries
Authors: Menozzi Grazia Chiara , Depaoli Alessandro , Ramella Marco , Alessandri Giulia , Frizziero Leonardo , De Rosa Adriano , Soncini Francesco , Sassoli Valeria , Rocca Gino , Trisolino Giovanni
Published: April 2024

(1) Background: Orthopedic surgery has been transformed by 3D-printed personalized instruments (3DP-PSIs), which enhance precision and reduce complications. Hospitals are adopting in-house 3D printing facilities, using cost-effective methods like Fused Deposition Modeling (FDM) with materials like Polylactic acid (PLA) to create 3DP-PSI. PLA’s temperature limitations can be overcome by annealing High-Temperature PLA (ann-HTPLA), enabling steam sterilization without compromising properties. Our study examines the in vivo efficacy of ann-HTPLA 3DP-PSI in pediatric orthopedic surgery. (2) Methods: we investigated safety and efficacy using ann-HTPLA 3DP-PSI produced at an “in-office” 3D-printing Point-of-Care (3DP-PoC) aimed at correcting limb deformities in pediatric patients. Data on 3DP-PSI dimensions and printing parameters were collected, along with usability and complications. (3) Results: Eighty-three ann-HTPLA 3DP-PSIs were utilized in 33 patients (47 bone segments). The smallest guide used measured 3.8 cm3, and the largest measured 58.8 cm3. Seventy-nine PSIs (95.2%; 95% C.I.: 88.1–98.7%) demonstrated effective use without issues. Out of 47 procedures, 11 had complications, including 2 infections (4.3%; 95% CI: 0.5–14.5%). Intraoperative use of 3DP-PSIs did not significantly increase infection rates or other complications. (4) Conclusions: ann-HTPLA has proven satisfactory usability and safety as a suitable material for producing 3DP-PSI in an “in-office” 3DP-PoC.
The Adhesion Characteristics and Aging Performance of Reversible Color-Changing Coatings for Self-Detection of Temperature by Power Equipment
Authors: Liu Guanke , Wang Wanwei , Dai Xiliang , Zhong Rongfu , Zhang Haipeng , Dong Bingbing
Published: April 2024

In order to detect abnormal heat generation in time, a reversible color-changing coating temperature measurement method is proposed for self-detection of temperature by power equipment, and its adhesion characteristics and aging performance were analyzed. The results showed that the reversible color-changing coating prepared with crystalline violet lactone as the colorant, bisphenol A as the color developer, octadecanol as the solvent, and RTV-II as the base paint can meet the requirements of self-detection of temperature by power equipment with its adhesion performance. The accelerated aging tests using high temperature, light and humidity were carried out in the laboratory, and we concluded that the deterioration degree of the color-changing coating was positively correlated with the temperature. Light can accelerate the aging rate of reversible color-changing coatings, and the degradation process of the coating was significantly accelerated under UV light. The effect of humidity on the coating was not significant. The degradation of the coating after aging for 288 h under indoor conditions was small, while it was accelerated under outdoor natural conditions. This research can provide a reference for the on-site application of reversible color-changing coatings for self-detection of temperature by power equipment.
Research on Dynamic Reactive Power Cost Optimization in Power Systems with DFIG Wind Farms
Authors: Xu Qi , Wang Yuhang , Chen Xi , Cao Wensi
Published: April 2024

As the power market system gradually perfects, the increasingly fierce competition not only drives industry development but also brings new challenges. Reactive power optimization is crucial for maintaining stable power grid operation and improving energy efficiency. However, the implementation of plant–grid separation policies has kept optimization costs high, affecting the profit distribution between power generation companies and grid companies. Therefore, researching how to effectively reduce reactive power optimization costs, both technically and strategically, is not only vital for the economic operation of the power system but also key to balancing interests among all parties and promoting the healthy development of the power market. Initially, the study analyzes and compares the characteristic curves of synchronous generators and DFIGs, establishes a reactive power pricing model for generators, and considering the randomness and volatility of wind energy, establishes a DFIG reactive power pricing model. The objective functions aimed to minimize the cost of reactive power purchased by generators, the price of active power network losses, the total deviation of node voltages, and the depreciation costs of discrete variable actions, thereby establishing a dynamic reactive power optimization model for power systems including doubly-fed wind farms. By introducing Logistic chaotic mapping, the CSA is improved by using the highly stochastic characteristics of chaotic systems, which is known as the Chaotic Cuckooing Algorithm. Meanwhile, the basic cuckoo search algorithm was improved in terms of adaptive adjustment strategies and global convergence guidance strategies, resulting in an enhanced cuckoo search algorithm to solve the established dynamic reactive power optimization model, improving global search capability and convergence speed. Finally, using the IEEE 30-bus system as an example and applying the improved chaotic cuckoo search algorithm for solution, simulation results show that the proposed reactive power optimization model and method can reduce reactive power costs and the number of discrete device actions, demonstrating effectiveness and adaptability. When the improved chaotic cuckoo algorithm is applied to optimize the objective function, the optimization result is better than 7.26% compared to the standard cuckoo search algorithm, and it is also improved compared to both the PSO algorithm and the GWO algorithm.
Fiber Solidification Treatment of River and Lake Wastewater and Sediments: Deformation Characteristics and Microscopic Mechanism Research
Authors: Yang Aiwu , Xu Jian , Gu Yuhao , Li Fengjun , Liu Xiaoqiang , Hou Jinfang
Published: April 2024

River and lake dredging projects inevitably produce significant quantities of wastewater and sediment. This accumulation results in dredged soil with high moisture content, characterized by low strength, rendering it unsustainable for use. To facilitate environmentally friendly utilization of wastewater and sediment, solidifying agents and basalt fibers are introduced to solidify the wastewater within the dredged sediment. This process transforms the wastewater, sediment, solidifying agents, and basalt fibers into a novel, strengthened material. This transformation allows for their application as stabilized soil for engineering endeavors. Indoor experiments and scanning electron microscope analyses were performed to examine the deformation characteristics of fiber-stabilized soil and analyze its micro-mechanisms. Research findings suggest that as the curing age increases, the curing agent’s reaction becomes more comprehensive. Fibers have the potential to ameliorate soil damage. The proposed binary-medium model’s applicability and accuracy were validated through the analysis of triaxial test results employing the reinforcement principle. These findings establish a theoretical foundation for the resourceful utilization of wastewater and sediment.
The Forgotten Language of Nontheistic Mysticism: Religious Factors in Erich Fromm’s Humanism
Authors: Pinkas Ronen
Published: April 2024

In You Shall Be as Gods, Erich Fromm (1900–1980) defines his position as nontheistic mysticism. This research clarifies the term, considers its importance within Fromm’s humanism, and explores its potential origins. The nontheistic mystical position plays a central role in Fromm’s understanding of the relationship between mysticism and organized religion, religion and religiosity, and it clarifies the relationship between religion, philosophy, and social psychoanalysis, whose combination constitutes his humanistic ethics. Nontheistic mysticism relates, as well, to Fromm’s understanding of human nature; it involves the question of the relationship between language, perception, and experience. The nontheistic mystical position is linked to Fromm’s negative theology, the x experience, and idolatry. Hence, the nontheistic mystical position is relevant to Fromm’s understanding of self-realization and his vision of a sane society. Unlike some scholarly opinion, the conclusions of this paper suggest that Fromm’s humanism is not radical, as long as radical is defined as an absolute atheistic secular feature that eliminates the range of religious language and experience. Rather, it is a broad and cautious humanism that, on the one hand, internalizes the transcendent divinity into the human subject and transforms it into anthropological–ethical phenomena, but, on the other, implies that atheism carries the risk of an idolatrous identification of the human being with God. Consequently, this humanism requires a religious–mystical component to adequately portray the spiritual and ethical potentials of humanity and its challenges. Nontheistic mysticism is a consciousness mechanism aimed at the fine-tuning of the individual’s moral compass, which is affected by the pathologies of normalcy that prevail in all societies.
Sounds, Emotions, and the Body in Pentecostal Romani Communities in Slovakia
Authors: Belišová Jana
Published: April 2024

In the past, the Romani in Slovakia identified with the prevailing religion, mainly with the Roman Catholic Church. However, the missionary activities of various Christian denominations after 1990 resulted in the conversion of the Romani to Pentecostal Christian communities. This launched a long, creative process of the formation of Pentecostal Romani music. Romani believers consider music and the ability to play and sing to be a gift from God and view these as a form of prayer that should serve for the praise of God. That is why many have given up their worldly music making and now play only praise songs. They gradually modified the hymns they borrowed and replaced them with their own creations. The soundscape of religion does not lie only in religious singing and music, as the emotional sermons and prayers, glossolalia and sounds during the healing and blessing rituals can also be considered religious sounds. During the worship services, this mixture of various sounds leads to the gradual spiritual and emotional unification of the community. The music and the rituals create feelings of intense sensory and emotional character that reflect in bodily expressions. Movements, dance, and the positions of the hands can help glorify God and experience the worship service more intensely. However, under certain circumstances, they might become sources of temptation and sin. This is related to the concepts of “purity” and “impurity”. The premises, whether sacral or profane, interior or exterior ones, also play a significant role in creating the sound. In writing this paper, I have also drawn on my own research on Romani Christian songs, which I carried out in (2012–2013 in Eastern Slovakia).
Psychedelic Mysticism and Christian Spirituality: From Science to Love
Authors: Cole-Turner Ron
Published: April 2024

The scientific claim that psychedelic drugs like psilocybin reliably occasion mystical experiences was justified using the Mystical Experiences Questionnaire (the MEQ), a survey first developed in the 1960s by Walter Pahnke using W.T. Stace’s Mysticism and Philosophy. Scholars in Christian mysticism reject the adequacy of Stace’s work for Western theistic mysticism, especially Christianity. One objection is that Stace follows William James in focusing on intense and unusual moments of mystical experience rather than the somewhat more ordinary mystical life. A greater concern is that Stace more adequately reflects non-Western traditions than Western theistic traditions like Christianity. For Stace, mysticism centers on the concept of union with external reality or with the absolute, a union in which the human creature is absorbed or fused. Christian mysticism, by contrast, involves a sense of presence rather than union, experienced in a most intimate relationship as a felt loving closeness with the divine, but not as fusion or absorption into the divine. While love of God is central to the Christian view, it is ignored in Stace and the MEQ30. Finally for Christianity, mysticism is not found in the momentary experience, but in the lifelong interpretation that leads to transformation.