bluuebunny/crossref_metadata_2025 huggingface dataset:
A revisão de literatura em teses de doutorado: análise de condução e redação
Authors: Castro Eliziane Rocha , Nunes João Batista Carvalho , Do Nascimento Francisco Jeovane , Rabelo Francy Sousa
Published: August 2020

Analisou-se a qualidade das revisões de literatura em teses de doutorado, cujas datas de defesa estão no interstício temporal 2013-2016 e são vinculadas ao Programa de Pós-Graduação em Educação e Contemporaneidade da Universidade do Estado da Bahia (PPGEduC/UNEB). Neste ensaio, mostram-se os resultados relativos a uma das linhas de pesquisa desse Programa. A demanda é ancorada numa abordagem mista ou quali-quantitativa, com adoção do método de pesquisa documental. As teses, consideradas documentos da pesquisa, foram obtidas no Catálogo de Teses e Dissertações da CAPES. Erigiram-se as análises com amparo na adaptação da Rubrica de Pontuação da Revisão de Literatura de Boote e Beile (2005), tomando-se quatro categorias: Cobertura, Síntese, Significância e Retórica. Os resultados desvelam que as teses examinadas fornecem aportes férteis e instigantes para estruturar a revisão de literatura na produção do conhecimento científico, porque apontam aspectos importantes que devem estar envolvidos para conduzir e escrever uma revisão de literatura de qualidade.
Governança e desempenho das universidades católicas à luz do Ranking Universitário Folha (RUF)
Authors: Calderón Adolfo-Ignacio , Wandercil Marco
Published: August 2020

Este estudo analisa, longitudinalmente, o surgimento e estabelecimento das universidades católicas no Brasil e aborda seu desempenho em termos de qualidade, a partir dos resultados no Ranking Universitário Folha (RUF). Realizou-se pesquisa documental, levantamento históricobibliográfico e análise de indicadores de desempenho do RUF com critérios quali-quantitativos. Utilizaram-se fontes de informações estatísticas, além de estudo sobre a forma de constituição de sua estrutura de governança. A pesquisa revela a importância do papel desempenhado pelas universidades católicas no cenário da Educação Superior, em praticamente todo o território nacional, evidenciada a partir da análise histórica, dentro de suas limitações e características confessionais. O estudo demonstra que, em termos de qualidade, a boa performance não é homogênea, pois 23,5% das universidades católicas apresentam desempenho fraco, ocupando as últimas posições no ranking estudado; por outro lado, existe uma elite de excelência de sete universidades que integram as 70 melhores universidades do país.
Dona Antonieta e os significados de uma vida como diretora do Ginásio de São Roque: uma perspectiva Weberiana
Authors: Lenk Tarina Unzer , Sandano Wilson
Published: August 2020

Este trabalho tem como objetivo a compreensão das ações sociais desempenhadas por Antonieta de Araújo Cunha, que atuou por mais de duas décadas na educação secundária da cidade de São Roque, SP, e na construção e direção do ginásio da cidade. Para compreender essas ações sociais, foi utilizada uma abordagem qualitativa da metodologia de história oral, tendo como base a história oral temática, tendo sido realizadas cinco entrevistas semiestruturadas com pessoas que tiveram convivência direta com a diretora ao longo do período em que ela atuou na administração da escola. O referencial teórico adotado nesta pesquisa foi a Sociologia Compreensiva de Max Weber, que fundamentou a compreensão da conduta humana dotada de sentido. Conclui-se que os significados vinculados às ações sociais executadas pela diretora Antonieta se sintetizam em categorias baseadas em valores de ordem, controle, autoridade, obediência e padrões estéticos e higiênicos, ainda presentes no imaginário social da comunidade local de São Roque.
Pengaruh Karakteristik Toko terhadap Pengalaman Konsumen Gerai Kopi
Authors: Br Sitepu Sri Nathasya , Christina Angelica Irene
Published: August 2020

This research attempts to examine the impact of the coffee shop characteristics towards the consumers experience when they visit the coffee shop. The coffee shop characteristics including functional, atmosphere, design, and social characteristics. The population of this study are all Surabaya productive age residents, and the sample of this study was determined using Quota Sampling and the Isaac and Michael formula with the respondents requirements are those who had been visiting and/or consuming products directly at Starbucks Surabaya on maximum of 2 -3 months before filling out the questionnaire, with total of 384 respondents needed to be obtained. The questionnaire was distributed online and offline, with total 369 questionnaires are used in this study. This research uses SEM analysis. This research found that only the functional and social characteristics of the coffee shop have significant effect on the experience gained by its consumers; while the atmosphere and design characteristics have no significant effect, as the design characteristics have negative effect on the consumers experience. The practical contribution of research for the coffee shop owner are to maintains functional and social aspects as well as, improving aspects of design characteristics and atmosphere so that consumers gain experience when visiting.
Performance Achievements of Family Business through Successor Readiness and The Relationship between Family and Business Members
Authors: Ongkowijoyo Gracia , Sutrisno Timotius F.C.W , Teofilus Teofilus
Published: August 2020

Family businesses are one of the biggest contributors to Indonesia’s gross domestic product (GDP). Universitas Ciputra facilitate their students with family business guild. Succession plan and communication are important aspects for family businesses. Family businesses must educate potential successors in order to implement the succession plan well. The purpose of this study was to examine the effect of preparation level of successors towards family business performance; examine the effect of relationships among family and business members towards family business performance. A quantitative approach was used with a population of Universitas Ciputra’s Family Business Community. Purposive random sampling technique was used, yeilding a total sample of 93 people. The data analysis method used is Partial Least Square (PLS). The results showed that preparation level of heirs variable has a positive and significant effect on family business performance; the relationship among family and business members variable has a positive and significant effect on family business performance.
ANALISIS TERHADAP FAKTOR YANG BERPENGARUH TERHADAP PENERAPAN KEBIJAKAN PENGELOLAAN SAMPAH DI YOGYAKARTA MENGGUNAKAN PEMODELAN SISTEM DINAMIS
Authors: Habibah Erpin , Novianti Febi , Saputra Hanafi
Published: August 2020

In  February  2019,  the  amount  of  waste  in  the  Piyungan,Yogyakarta, landfill  area,  was  increased.  The  heap  of  waste  harms  to  many  fields,  especially  to environmental  health.  Data  from  the  Department  of  Public  Works  Housing  and Energy Mineral Resources show that the volume of waste was increased meanwhile the  number  of  landfill  areas  was  significantly  decreased,  especially  from  2017  to 2018.  If  there  is  no  proper  solution,  this  issue  can  be  a  continuous  problem  and  a terrible  disaster.  The regulations about waste management had been built by government, even some  programs  in  waste  management  have  been launched to solve the waste problem. Nevertheles the real conditions did not accord with the goals. This descriptive study uses statistical methods for processing secondary and primary data which are analyzed based on dynamics system models. The results of this study indicate that the Yogyakarta waste management system is not good enough. The several influencing factors are the government policy is not yet fully known by the whole community, cultural attitudes and awareness of the community to care for waste are still low, and the landfill facilities are still  inadequate.

 

Keywords: Landfill, Public Awareness, Regulation, Waste  Management, Dynamics System.

 

Abstrak

Pada Februari 2019 telah terjadi pembengkakan jumlah sampah di area TPA Piyungan, Yogyakarta. Data Dinas Pekerjaan Umum Perumahan dan Energi Sumber daya Mineral, menunjukkan selama kurun waktu 2015-2018 terdapat peningkatan jumlah volume sampah yang dihasilkan, sementara jumlah TPA mengalami penurunan yang signifikan terutama dari tahun 2017 ke tahun 2018. Pola kontradiktif ini akan menjadi masalah berkelanjutan dan bahkan bisa menjadi bencana mengerikan apabila tidak ada penanganan tepat sasaran. Kebijakan pemerintah sampai saat ini sudah ada, bahkan beberapa program sudah dicanangkan untuk mengatasi permasalahan mengenai sampah. Meskipun demikian, kondisi di lapangan ternyata tidak sesuai dengan harapan dan rencana pemerintah. Penelitian deskriptif ini menggunakan metode statitik untuk pengolahan data sekunder dan primer kemudian dianalisis berdasarkan pemodelan sistem dinamis. Hasil  penelitian  menunjukkan  bahwa sistem pengelolaan sampah Yogyakarta  belum  cukup  baik. Beberapa faktor  yang  mempengaruhi  diantaranya kebijakan pemerintah belum diketahui secara menyeluruh, budaya peduli sampah dan  kesadaran masyarakat masih rendah,  serta fasilitas TPA belum memadai.

 
Kata kunci: Kesadaran Publik, Pengelolaan sampah, Regulasi, Tempat pembuangan akhir, Sistem Dinamik.
MODAL SOSIAL DALAM REVITALISASI KEARIFAN LOKAL (STUDI KASUS DESA WISATA KANDRI KECAMATAN GUNUNG PATI KOTA SEMARANG)
Authors: Jannati Syifa Ayyada , Ramadhan Dani , Pertiwi Cindy Nadya Dewi
Published: August 2020

Cities with various activities have a rapid change in every part of them. This change slowly began to threaten the value of local wisdom in society. It getting worst by globalization that will change the value of society and make them individualistic in this digital era. The revitalization for original culture through make a tourist village that will be the place for development local wisdom which is getting fade between village society in this globalization era is the right thought to solve this problem. The one of tourist village that elevate local wisdom is Kandri Tourist Village. Qualitative method has been selected by researchers to get data through interview an obsevation in research location. Social capital theory used by researches is the theory that was coined by Putnam. The goal of this research to give the knowledge to reader that tourist village notonly empowering society, but tourist village can elevate local wisdom that left out by this generation. The result in the process to realize culture revitalization and empowering other, have the different caracter is must that is local wisdom and have a leader as the developer to develop Kandri  tourist village.

 

Keywords: Tourist Village, Local Wisdom, Social Capital.  

 

Abstrak

Kota dengan berbagai hiruk pikuk yang ada di dalamnya mengalami perubahan pesat. Perkembangan ini secara perlahan mulai mengancam nilai kearifan lokal yang ada di masyarakat. Kondisi seperti ini diperparah dengan munculnya serbuan globalisasi yang semakin hari mulai mengubah tatanan masyarakat yang lebih individualis di era digital seperti sekarang ini. Dalam upaya untuk merevitalisasi budaya luhur yang telah ada, salah satu cara yang tepat dengan menggunakan desa wisata sebagai wadah guna merevitalisasi kearifan yang mulai luntur di masyarakat. Salah satu desa wisata yang mengangkat kearifan lokal yaitu Desa Wisata Kandri. Penelitian menggunakan metode kualitatif dengan pengambilan data menggunakan wawancara dan observasi. Teori modal sosial yang digunakan adalah teori yang dicetuskan oleh Putnam. Tujuan penelitian ini digunakan untuk memberikan pemahaman bahwa dengan adanya Desa Wisata Kandri tidak hanya memberikan pemberdayaan tetapi ikut merevitalisasi kearifan lokal yang mulai ditinggal generasi sekarang. Hasil penelitian menunjukkan dalam mewujudkan revitalisasi budaya dan pemberdayaan harus memiliki karakter yang membedakan yaitu kearifan lokal serta memiliki pemimpin sebagai pelaksana segala wujud pengembangan Desa Wisata Kandri.

 

Kata kunci : Desa Wisata, Kearifan Lokal, Modal Sosial.
An Unusual Late Aboriginal Assemblage from the Wilson Site (41SS186), San Saba County, Central Texas
Authors: Hixson Charles
Published: August 2020

The late aboriginal component in the Wilson Site in San Saba County is unusual in that most of the assemblage is consistent with that of Classic Toyah, but the diagnostic projectile point is an unnotched triangular arrow point instead of the typical Perdiz point. The absence of Perdiz points suggests that this component is associated with non-Toyah people and possibly dates to after 1700. Archaeological testing by the Llano Uplift Archeological Society (LUAS) to find supporting evidence for a historic date identified an Austin phase shell midden and a “Late Component” composed of triangular arrow points, end scrapers, a beveled biface and bone-tempered sherds, but no items of European manufacture. Complicating matters, the luminescence dating on a ceramic sample opens the possibility that the Late Component predates the currently accepted beginning of the Toyah phase.
Ethnohistoric Records of Hunter-Gatherer Diet of the Texas/Mexico Borderlands: Implications for Staple Foods of the Lower Pecos Canyonlands During the Holocene
Authors: Riley Tim
Published: August 2020

Hinds Cave (41VV456) and other rockshelters excavated in the Lower Pecos Canyonlands have yielded thousands of coprolites spanning the Holocene. To date, several hundred specimens have been analyzed, providing a detailed record of meals consumed by hunter-gatherers who called this landscape home. This article compares the paleodietary records derived from these specimens with the foodways documented in the ethnohistoric records available for the Lower Pecos Canyonlands and adjacent landscapes. This comparison confirms the deep temporal roots of the foodways recorded in the earliest written records of the Texas/Mexico borderlands. Coprolite data corroborate the strong dependence on the seasonal staples of lechuguilla (Agave lechuguilla), nopales (Opuntia sp.), and tunas (Opuntia sp.) observed in the ethnohistoric literature. The temporal endurance of this subsistence strategy suggests that there may be some components of this dietary pattern that could inform on many of the diet-related health issues observed among modern Native American and other populations.
Spanish Edition: Chasing the Phantom Ship: Revisiting Interpretations of the Boca Chica No. 2 Shipwreck on the Texas Coast
Authors: Borgens Amy , Hoyt Steven
Published: August 2020

La playa de Boca Chica se extiende aproximadamente 12 kilómetros a lo largo la costa sur de Texas, en el condado de Cameron, entre el paso de Brazos Santiago y la desembocadura del río Bravo (río Grande) en la frontera entre Texas y México. Se tienen noticias del naufragio de más de 165 barcos históricos a lo largo de la costa sur de Texas, de los cuales, al menos cuatro o parte de ellos, han sido descubiertos hasta el momento. El más conocido de estos naufragios es el pecio 41CF184, apodado Boca Chica No. 2, que ha adquirido un estatus casi mitológico en la región, ya que durante mucho tiempo se le ha vinculado de forma circunstancial al buque de guerra mexicano Moctezuma, el cual, no por casualidad, resulta ser uno de los naufragios más famosos de la región. ¿Es Boca Chica No. 2, el famoso buque de guerra que, en alguna ocasión, se consideró un “buque fantasma” debido a la frecuencia con la que eludía a las patrullas texanas? La evidencia sugiere lo contrario, pero la importancia, tanto de la nave histórica como del yacimiento arqueológico, invita a reexaminar este misterio no resuelto.
History of Archeological Investigations at Palo Duro Canyon State Park
Authors: Arias Veronica , Lyle Anthony , Shaller Rolla
Published: August 2020

Palo Duro Canyon has been an important locale for human occupation with its distinct topography, fauna, and flora from the Paleoindian inhabitants to those of the Historic Period. There is archeological evidence of human habitation at Palo Duro Canyon throughout the past 12 millennia. Native Americans who lived in and around the canyon had access to resources not easily found on the adjoining upland plains. The canyon provided an abundance of sheltered camping and year-round supply to water, wood, stone tool materials, game, and wild plant resources. The bordering uplands, covered with grass and dotted with playa lakes, afforded campsites with good visibility and access to game such as bison, antelope, and waterfowl. This article reviews the history of archaeological work conducted at Palo Duro Canyon State Park since the park opened in 1934. It examines how evolving site recording standards, archaeological methods, and construction projects have influenced the types of archaeological investigations that have been conducted within the park over time. These investigations have occurred within the context of larger changes to the discipline and, more recently, expansions to the original park boundaries. Together, they help elucidate on the rich history of human occupation in the region.
Assessing subsidence of Mexico City from InSAR and LandSat ETM+ with CGPS and SVM
Authors: Poreh Davod , Pirasteh Saied , Cabral-Cano Enrique
Published: August 2020

This study presents an enhanced analysis of the subsidence rates and their effects on Mexico City. As a result of excess water withdrawal, Mexico City is experiencing subsidence. To analyze this subsidence of Mexico City, we integrated and analyzed Interferometric Synthetic Aperture Radar (InSAR), Continuous Global Positioning Systems (CGPS), and optical remote sensing data. This study utilized 52 ENVISAT-ASAR, nine GPS stations, and one Landsat ETM+ image from the Mexico City area to gather a better understanding of the subsidence rates and its effects on Mexico City’s community. The finding of this study reveals a high amount of correlation (up to 0.98) between two independent geodetic methods. We also implemented the Support Vector Machine (SVM) analysis method based on Landsat ETM+ image to classify Mexico City’s population density. We used SVM to compare Persistent Scatterer Interferometry (PSI) subsidence rates with the buildings’ distribution densities. This integrated study shows that the fastest subsidence zone (i.e. areas greater than 100 mm/yr), which falls into the above-mentioned temporal baseline, occurs in the areas of high and moderate building distribution density.
How Different Health Literacy Dimensions Influences Health Status and Well-being among Men and Women: The Mediating Role of Health Behaviors
Authors: Zhang Fan , Or Peggy Pui-Lai , Chung Joanne Wai-Yee
Published: August 2020

Background. Health literacy, the ability to access, understand, evaluate, and apply health information, was found to contribute to positive health outcomes, possibly via promoting healthy behaviors. However, the specific pathways linking different health literacy skills to health and well-being has remained unclear. Methods. A cross-sectional survey with structural questionnaires was administered among 2236 community-based adults in Hong Kong (mean age = 46.10 ±19.05). Health literacy was measured by HLS-Asian-47. Participants' health behavior, physical conditions, and subjective well-being were reported.Results. With structural modeling path analysis, health literacy in finding and understanding information showed a direct effect on enhancing physical health. While applying information capacity had an indirect positive effect via promoting health behaviors, which was moderated by sex. Only among women, this indirect effect predicting fewer physical symptoms and better well-being was significant.Conclusions. Although similar patterns were found in physical condition and well-being, distinct direct and indirect pathways were found of different health literacy dimensions for men and women. Based on the findings, by targeting specific health literacy skills, education programs should be developed to enhance women’s health knowledge, and men's application of the knowledge in healthy lifestyle.
Labial Gingival Thickness Assessment at Mandibular Incisors of Orthodontic Patients With Ultrasound and Cone-Beam Ct. A Cross-Sectional Study
Authors: Kloukos Dimitrios , Kakali Lydia , Koukos George , Sculean Anton , Stavropoulos Andreas , Katsaros Christos
Published: August 2020

Background: Quantitative and qualitative analyses of several periodontal parameters play an important role in several dental procedures. Aim of the current study was to assess gingival thickness (GT) at mandibular incisors of orthodontic patients with two methods and determine how these methods are compared to each other when assessing periodontal anatomy through soft tissue thickness.Methods: The sample consisted of 40 consecutive adult orthodontic patients. GT was measured at both central mandibular incisors, mid-facially on the buccal aspect, 2mm apically to the free gingival margin with two methods: a) clinically with an Ultrasound device (USD) and b) radiographically with Cone Beam Computed Tomography (CBCT). Results: CBCT measurements were consistently higher than USD measurements, with the difference ranging from 0.13 mm to 0.21 mm. No difference was noted between the repeated CBCT measurements at the right central incisor (Bias= 0.05 mm; 95% CI= -0.01, 0.11, p=0.104). Although the respective results for the left incisor indicated, statistically, that the measurements were not exactly replicated, the magnitude of the point estimate was small and not clinically significant (Bias= 0.06 mm; 95% CI= 0.01, 0.11, p=0.014). Small differences between CBCT measurements made by the 2 examiners at the left central incisor (bias= 0.06 mm, 95% CI= 0.01, 0.11, p=0.014) were detected. However, this difference was minor, and again, not clinically significant. The respective analysis on the right incisor showed no significant difference (bias= 0.05 mm, 95% CI= -0.01, 0.11, p=0.246). Conclusions: In the present study GT was assessed clinically with in-situ measurements and radiographically after computer-aided appraisal. Based on the reproducibility, CBCT imaging proved to be at least as reliable as the ultrasound determination, but yielded higher values than the USD measurements.
Sexual Coercion at sexual debut and associated factors among Young Females in rural areas of Boset District, Eastern Ethiopia: A Mixed Method Study
Authors: Abeya Sileshi Garoma
Published: August 2020

Background

The sexual behavior and experience of young people in developing countries have considerable attention. Despite growing recognition of the problem, relatively little is known about the issue of coercive sex in rural settings in developing countries including Ethiopia.
Objectives

Assess the prevalence and associated factors of sexual coercion at sexual debut among young females in rural areas of Boset district, Oromia Region, Eastern Ethiopia.
Methods

Cross-sectional community based study was conducted using both qualitative and quantitative data collection methods. A sample of 632 young females aged 10–24 years was taken from six rural Kebeles by systematic random sampling procedure for quantitative and data were collected using a pre-tested structured questionnaire. The collected data were analyzed using SPSS version 23. Principally Binary Logistic regression model was fitted. Moreover, eight focus group discussions targeting different groups were held and analyzed thematically.
Results

The prevalence of sexual coercion at sexual debut was 36.5% among sexually active respondents. Married young female (COR, 0.39; 95%CI: 0.21,0.71), living alone (away from their parents (AOR, 5.07; 95%CI: 1.07,24.01), age (AOR, 0.19; 95%CI: 0. 064, 0.544), alcohol consumption (AOR, 2.44; 95%CI: 1.17, 5.08) and Khat chewing (AOR, 8.30; 95%CI: 1.892, 36.378) were factors predicting the likelihood of having sexual coercion at sexual debut.
Conclusion

Sexual coercion at sexual debut is a major public health problem among young females in the study community. The emerging program interventions need to take this problem into consideration.
Cell-free long non-coding RNA-H19 and miRNA-29a, miRNA-29b expression in Type 2 Diabetes Mellitus patients.
Authors: Alfaifi Mohammed , Verma Amit Kumar , Alshahrani Mohammed Yahya , Joshi Prakash C , Alkhathami Ali Gaithan , Ahmad Irfan , Hakami Abdulrahim Refdan , Beg Mirza Masroor Ali
Published: August 2020

Background: Type 2 diabetes mellitus [T2DM] has been one of the common diseases and characterized by increased blood glucose levels. T2DM participates in several organ damages as well revealed that cell-free non-coding RNAs and microRNAs (miRNAs) have been demonstrated to serve as important diagnostic/prognostic biomarkers in the pathophysiology of diabetes.Materials/Methods: The present study included clinically confirmed untreated newly diagnosed 200 cases of T2DM and 200 healthy subjects. Blood samples collected in fluoride vials were used to check blood glucose for fasting and after 2 hours of breakfast (postprandial), blood samples collected in EDTA vials used for the Hba1c level.Blood samples from all the participants were collected in plain vials used for cell-free total RNA extraction. Total extracted RNA was quantified and 100ng was used to synthesized the cDNA for cell-free lncRNA H19, miRNA-29a, and miRNA-29b expression using specific primers/ probes using quantitative real-time PCR method. Serum Biochemical parameters were analyzed after collection of the sample to observe the changes among T2DM cases and healthy controls. Subjective data were recorded to evaluate the association with lncRNA H19, miRNA-29a, and miRNA-29b expression among T2DM cases. Results: It was observed that type 2 diabetic patients had decreased [0.59 fold] lncRNA H19 expression while increased miRNA-29a [5.62 fold] and miRNA-29b [5.58 fold] expression. Decreased expression of lncRNA H19 was observed to be associated with gender [p=0.004], hypertension [p<0.0001], weight loss [p=0.02] and fatigue [p=0.02]. Increased miRNA29a expression was linked with hypertension [p<0.0001], alcoholism [p=0.04], and smoking [p<0.0001] as well as miRNA-29b expression was associated with hypertension [p=0.0001], weight loss [p=0.002], smoking [p=0.0002], alcoholism [p<0.0001]. Low [<1 fold] and high [>1 fold] expression of lncRNA H19 expression was linked with miRNA-29a [p=0.005] and miRNA-29b [p<0.0001] expression. lncRNA H19 expression showed negative correlation with miRNA-29a expression [r= -27, p<0.0001] and miRNA-29b [r= -47, p<0.0001]. Conclusion: The present study concluded that lower lncRNA H19 expression, and increased miRNA-29b, miRNA-29b expression associated with the severity of T2DM patients. Decreased lncRNA H19 expression, and increased miRNA-29b, miRNA-29b expression observed to be interrelated with clinicopathological findings of T2DM patients could involve in pathogenesis disease.
NMFNA: a non-negative matrix factorization network analysis method for identifying communities and characteristic genes from multi-type pancreatic cancer data
Authors: Ding Qian , Sun Yan , Shang Junliang , Li Feng , Zhang Yuanyuan , Liu Jin-Xing
Published: August 2020

Background: Pancreatic cancer (PC) is a common type of digestive system disease. Comprehensive analysis of multiple types of PC genetic data plays a crucial role in understanding its potential biological mechanisms. Currently, Non-negative Matrix Factorization (NMF) based methods are widely used for data analysis. Nevertheless, it is a challenge for them to integrate and decompose multiple types of data simultaneously.Results: In this paper, a Non-negative Matrix Factorization Network Analysis method, NMFNA, is proposed, which introduces a graph regularized constraint to NMF, for identifying communities and characteristic genes from multi-type PC data. Firstly, three PC networks, i.e., methylation network (ME), copy number variation network (CNV), and the bipartite network between them, are constructed by both ME and CNV data of PC downloaded from the TCGA database, using the Pearson Correlation Coefficient. Then, the NMFNA is proposed to detect core communities and characteristic genes from these three PC networks effectively due to its introduced graph regularized constraint, which is the highlight of NMFNA. Finally, both gene ontology enrichment analysis and pathway enrichment analysis are performed to deeply understand biological functions of detected core communities.Conclusions: Experimental results demonstrated that the NMFNA facilitates the integration and decomposition of multiple types of PC data simultaneously and can serve as an alternative method for detecting communities and characteristic genes from multiple genetic networks. The data and demo codes of NMFNA are available online at https://github.com/CDMB-lab/NMFNA.
Morphology, morphogenesis and phylogeny of the new soil ciliate, Bistichella granulifera n. sp. (Protista, Ciliophora)
Authors: Lyu Zhao , Li Jingbao , Wang Yurui , Ma Jiyang , Shao Chen
Published: August 2020

Background: Hypotrichous ciliates are showing extremely morphological diverse and complicated morphogenesis. However, many hypotrichs are still unknown/poorly-known and need to be explored. Recently, Foissner (2016) reassigned the species in Bistichella and Parabistichella. Due to lack of morphological and morphogenetic information, some problems were overlooked. In present work, a new species, Bistichella granulifera n. sp., was found in northern China. Based on its infraciliature, ontogenesis analyses and phylogenetic study, the phylogenetic position of Bistichella was discussed.Results: Bistichella granulifera n. sp. was characterized by having elongated oval body, two macronuclear nodules, cortical granules present, three frontal and three or four buccal cirri, three frontal rows with seven to ten cirri, usually two frontoventral rows with the left one usually terminates at 90% down length of body and no breaks in the right one. The main morphogenetic features of the novel species were as follows: (1) the posterior part of the old adoral membranelles is renewed; (2) frontoventral-transverse cirral anlagen III to V each form a frontal row, and anlagen VI to n each produce a frontoventral row; (3) both marginal rows and dorsal kineties develop intrakinetally; (4) the macronuclear nodules fuse to form a single mass. The gene sequence of Bistichella was investigated for the first time. Phylogenetic analyses based on the SSU rDNA sequences showed that the Bistichella groups with the non-dorsomarginalian hypotrichs.Conclusions: Morphology, morphogenesis and phylogenetic position of Bistichella granulifera n. sp., was investigated. Each frontoventral row originates from one anlage and only three bipolar dorsal kineties are present in the new species, which means it should belong to non-Dorsomarginalia rather than Amphisiellidae. Even though Bistichella has a close relationship with amphisiellids, due to the limited gene sequence, the phylogenetic position of Bistichella needs more data to be fully understood.
Collective Advanced Life Support Ambulance An Innovative Transportation of Critical Care Patients by Bus in COVID-19 Pandemic Response
Authors: Lentz Thierry , Groizard Charles , Colomes Abel , Ozguler Anna , Baer Michel , Loeb Thomas
Published: August 2020

Background: During the COVID-19 pandemic, as the number of available Intensive Care beds in France did not meet the needs, it appeared necessary to transfer a large number of patients from the most affected areas to the less ones. Mass transportation resources were deemed necessary. To achieve that goal, the concept of a Collective Advanced Life Support Ambulance (CALSA) was proposed in the form of a long-distance bus re-designed and equipped so as to accommodate up to six intensive care patients and allow Advanced Life Support (ALS) techniques to be performed while en route.Methods: The expected benefit of the CALSA, when compared to ALS ambulances accommodating a single patient, was to reduce the resources requirements, in particular by a lower personnel headcount for several patients being transferred to the same destination. A foreseen prospect, comparing to other collective transportation vectors such as airplanes, was the door-to-door capability, minimalizing patients’ handovers for safety concerns and time efficiency. With the project of a short-distance transfer of several Intensive Care Unit (ICU) patients together, the opportunity came to test the CALSA under real-life conditions and evaluate safely its technical feasibility and impact in time and resources saving, before it could be proposed for longer distances.Results: Four COVID-19 patients were transported over 37 km. All patients were intubated and under controlled ventilation. One of them was under Norepinephrine support. Mean loading time was 1min 39s. Transportation time was 29 min. At destination, the mean unloading time was 1min 15s. No serious adverse effect, in particular regarding hemodynamic instability or ventilation disorder, has been observed. No harmful incident, in particular no accidental extubation, has occurred.Conclusions: It was a very instructive test. Collective medical evacuation by bus for critically ill patients under controlled ventilation is suitable and easy to implement. Design, ALS equipment, power autonomy, safety and resources saving, open the way for carrying up to 6 ICU-patients over a long distance. The CALSA could bring a real added-value in an epidemic context and could also be helpful in many other events generating multiple victims such as an armed conflict, a terrorist attack or a natural disaster.
Happy Hypoxia: Higher NO in red blood cells of COVID-19 patients
Authors: Moratz Esmaeil , Malekmohammad Majid , Jamaati Hamidreza , Naghan Parisa Adimi , Hashemian Mohammadreza , Tabarsi Payam , Varahram Maohammad , Zaheri Hamidreza , Chousein Efsun Gonca Uğur , Folkerts Gert , Adcock Ian
Published: August 2020

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19) has spread to almost 100 countries, infected over 10M patients and resulted in 505K deaths worldwide as of 30th June 2020. The major clinical feature of severe COVID-19 requiring ventilation is acute Respiratory Distress Syndrome (ARDS) with multi-functional failure as a result of a cytokine storm with increased serum levels of cytokines. The pathogenesis of the respiratory failure in COVID-19 is yet unknown, but diffuse alveolar damage with interstitial thickening leading to compromised gas exchange is a plausible mechanism. Hypoxia has been seen in the COVID-19 patients however, patients present with a distinct phenotype. Intracellular levels of NO playing important role in the vasodilation of small vessels.Objectives: To elucidate the intracellular levels of NO inside of RBCs in COVID-19 patients compared with that of healthy control subjects.Methods: We recruited 14 COVID-19 infected cases who had pulmonary involvement of their disease, 4 non-COVID-19 healthy controls (without pulmonary involvement and were not hypoxic) and 2 hypoxic non-COVID-19 patients subjects who presented at the Masih Daneshvari Hospital of Tehran, Iran between March-May 2020. Whole blood samples were harvested from patients and intracellular levels of NO in 1 million red blood cells (RBC) was measured by DAF staining using flow cytometry (FACS Calibour, BD, CA, USA).Results: The Mean florescent of intensity for NO was significantly enhanced in COVID-19 patients compared with healthy control subjects (P≤0.05). As a further control for whether hypoxia induced this higher intracellular NO, we evaluated the levels of NO inside RBC of hypoxic patients. No significant differences in NO levels were seen between the hypoxic and non-hypoxic control group.Conclusions: This pilot study demonstrates increased levels of intracellular NO in RBCs from COVID-19 patients. Future studies should examine whether intracellular NO would be increased in large number of COVID-19 patients for usage of possible NO therapy in severe patients.
Chronic Social Stress Impairs Prefrontal Cortical Myelination in Juvenile Mice
Authors: Sun Xuan , Yu Qiu-Hui-Hong , Luo Fei , Li Bao-Ming
Published: August 2020

The prefrontal cortex (PFC) is a key brain region mediating many cognitive functions, and its structures and functions are particularly vulnerable to stress. Chronic social defeat stress (CSD) has been proposed as a model of anxiety/depressive-like behaviors. In the present study, we examined whether CSD affects the myelination in the medial PFC (mPFC) in mice. Our results show that CSD dramatically reduced the myelin basic protein (MBP) staining in the mPFC, with no effect on the MBP-labeling in the motor cortex, striatum, hippocampus and corpus callosum. Consistently, the CSD mice demonstrated a significant increase in oligodendrocyte precursor cells (PDGFRα+ cells) and a significant decrease in mature oligodendrocytes (CC1+/Olig2+ cells) in the mPFC. The present study demonstrates that CSD impairs the differentiation of oligodendrocytes and myelination in the mPFC, suggesting a potential mechanism for stress-induced change in PFC-dependent behaviors.
A Qualitative Exploration of Racial and Ethnic Differences in Cost of Care Conversations Among Older Adults
Authors: Conner Kyaien Oquinn , Wiltshire Jaqueline , Garcia Edlin Colato , Langland-Orban Barbara , Anderson Erica , Carrion Iraida , Goodman Amber , Goodman Ashley
Published: August 2020

Background: The rapidly growing racially diverse, aging population in the United States is presenting unique challenges for our social, economic, and healthcare systems. Rising health care costs, increased patient cost-sharing, and limited financial resources make this generation of older Americans susceptible to large medical bills or debt which disproportionally impacts older racial/ethnic minorities. Cost-of-care (CoC) conversations between patients and doctors is one recommended approach to containing health care costs and alleviating patients’ financial burden of care.Methods: The current study used focus group methodology to qualitatively explore the contextual factors that influence CoC conversations in a diverse sample of older adults (N=27). Results: Three focus groups were held with White (n=10), African American (n= 9) and Hispanic/Latino (n=8) participants. Thematic analysis yielded four broad themes. Results suggest that CoC conversations are not occurring with physicians, although conversations are occurring with dentists and pharmacists. Contributors to CoC conversations included positive provider affect, rapport/relationship building, and communication. Barriers included: new age physicians, lack of physician training, wait time, lack of focus on the patient, language, provider preferences, fear of physicians, and religion; however, there was little similarity across racial/ethnic groups.Conclusions: The results of this qualitative study suggest that cost-of-care conversations are not occurring between providers and their patients. Several barriers, contributors, and solutions to cost-of-care conversations were identified by focus group participants, which have important implications for the field, and are addressed in this manuscript.
Metallized ε-FeOOH and the heterogeneous electrical conductivity structure in the lower mantle
Authors: Zhuang Yukai , Cui Zhongxun , Tang Ruilian , Tao Renbiao , Hou Mingqiang , Li Kuo , Chen Jiuhua , Hu Qingyang
Published: August 2020

Electrical heterogeneity at the depth of 900-1400 km in Earth’s interior is a key factor to constrain the minor phase composition of the lower mantle. However, prevailing mineralogical models including Fe- or Al-enriched silicates or ferropericlase are insufficient to explain the ultra-high electrical conductivity in local areas of subduction slabs. Here, we measure the electrical conductivity of ε-FeOOH up to 61 GPa. A 3-order abrupt jump of electrical conductivity is observed in 45-50 GPa, reaching 1.24±0.19 × 103 S/m at 61 GPa. Density mean field theory simulations suggest that ε-FeOOH undergoes a Mott-type electronic transition, which leads the conduction mechanism to switch from small polaron conduction to free electron conduction. Compared with bridgmanite, ferropericlase and conventional mantle compositional models, the electrical conductivity of the metallic ε-FeOOH is 1-3 orders of magnitude higher. Minor or moderate incorporation of metallic ε-FeOOH into the ambient lower mantle could reproduce the observed electrical heterogeneity derived from geomagnetic data at 900-1400 km depth.
Evaluation of the Chemo-Radiotherapy Effect on the Level of Expression of miR-374 Gene Expression in Rectal Cancer Samples
Authors: Ahmadi Azam , Bayatiani Mohamd Reza , Seif Fatemeh , Ansari Jamshid , Rashidi Parisa , Moghadasi Mona , Etemadi Mobarake
Published: August 2020

The authors have requested that this preprint be removed from Research Square.
Leaf δ15N, δ13C and Their Associations with Soil Fungal Biodiversity, Ectomycorrhizal and Plant Pathogenic Abundance in Forest Ecosystems of China
Authors: Song Wenchen
Published: August 2020

The authors have withdrawn this preprint due to author disagreement.
WITHDRAWN: A Systematic Review of the Literature Regarding Physical, Cognitive and Emotional Outcomes in Older Adults Practitioners of Physical Exercise
Authors: Araque-Martínez Miguel Ángel , Artés-Rodríguez Eva María , Casimiro-Andújar Antonio Jesús , Ruiz-Montero Pedro Jesús
Published: August 2020

Background: This systematic review provides an overview about those papers with a specific physical exercise intervention aimed to improve physical, cognitive and/or emotional outcomes in healthy older people aged 60 or over. Studies with no intervention whose sample were active healthy older adults were also included.Methods: An exhaustive literature search was done through two databases, including studies from January 2000 to July 2020. Results: Of the 2006 identified records, 66 met the inclusion criteria and were selected. Results from the review showed a wide variety of older adults and lengths of the interventions, from five weeks (the shortest one) to five years. Aerobic exercise, either isolated or combined with strength performance, was also the most common type of exercise recognized in this systematic review.Conclusions: Findings also suggest that the number of studies with all, physical, cognitive and emotional outcomes have been increasing during recent years in healthy older adults.
Telemedicine Intervention Efficiency on Mobility Recovery After Bariatric Surgery: The MyGoodTrip Randomized Controlled Trial
Authors: Bruzzi Mathieu , Carette Claire , Puerto Katia Lurbe i , Poghosyan Tigran , Bretault Marion , Vilfaillot Aurélie , Chatellier Gilles , Chevallier Jean-Marc , Czernichow Sébastien , Rives-Lange Claire
Published: August 2020

Background: The use of telemedicine may improve mobility recovery in patients with severe obesity undergoing bariatric surgery. Our aim was to evaluate a telemedicine intervention program dedicated to the promotion of physical activity including teleconsultation and telemonitoring on patients’ number of steps in the six first months following bariatric surgery. Methods: This study was an open label randomized controlled trial. Patients were included during the first week after bariatric surgery. Patients were randomized in two groups of telemedicine intervention: (i) physical coaching focusing on mobility (=TelePhys group) or (ii) dietary coaching (=TeleDiet group). The primary outcome was the difference in the delta number of steps measured during a period of 14 days at the first and sixth postoperative months between the TelePhys and the TeleDiet groups. Data were collected using the connected wireless watch pedometer. Body weight evolution and health-related quality of life were also evaluated. An additional qualitative approach was conducted to evaluate patients’ perception of the connected devices used in the trial.Results: Ninety patients with mean age (SD) 40.6 years (±10.3) were included. Seventy-three patients were females (81.1%) and 62 had gastric bypass (69%). The two intervention groups were comparable at inclusion except for the number of steps significantly higher in the TelePhys group (1073.102 ± 471.102 versus 839.102 ± 437.102, p=0.045). An increase of the mean number of steps between the first and the sixth month was found in both groups but this delta was significant only in the TeleDiet group (p=0.010). No difference was found when comparing the delta between the two intervention groups. A significant increase in quality of life was observed in both groups without any significant differences between the two interventions. Conclusions: Our study was not able to show a significant superiority of a telemedicine intervention dedicated to physical activity in mobility recovery after bariatric surgery. The early postoperative time frame for our intervention may explain the null findings. Further research will need to focus on long-term interventions.Trial registration: This study was registered the 23th of March 2016 (ClinicalTrials.gov Identifier: NCT02716480).
Sleep and Its Importance for Health-Related Quality of Life in 3–10-Year-Old Children
Authors: Sundell Anna Lena , Angelhoff Charlotte
Published: August 2020

Background: Considering the reports of increasing sleep problems in children, affecting health and well-being in young children and their families, we found it important to gain more knowledge about sleep and its correlation to health-related quality of life (HRQoL) in young, healthy children. The aim with this study was to describe sleep quality, sleep duration, and HRQoL in healthy 3–10-year-old children and to test associations between children’s sleep and HRQoL. Methods: Parents of 160 children (average age: 6.9 years, SD ±2.2) participated in the study. Sleep onset problems (SOP), sleep maintenance problems (SMP), and sleep duration were measured by the Pediatric Insomnia Severity Index (PISI). KIDSCREEN-27 was used to measure HRQoL in five dimensions: physical well-being, psychological well-being, autonomy and parent relation, social support and peers, and school environment. Results: The average score was 2.2 for SOP (SD +/-2.2) and 1.3 for SMP (SD +/-1.6). Almost all children (98%) slept between 8 and 13 hours per night. Younger children had statistically significantly more sleep problems than older children. Correlations were found between SOP and poor psychological well-being (p < 0.05, ρ = - 0.16), and between SMP and poor psychological well-being (p < 0.05, ρ = - 0.21), poor school environment (p < 0.01, ρ = - 0.29), and poor social support and peers (p < 0.05, ρ = - 0.19). Conclusion: Children’s sleep associates with psychosocial well-being, school functioning and relations to peers, and need to be acknowledged in child health care settings and schools.