bluuebunny/crossref_metadata_2025 huggingface dataset:
The aspects of the transdisciplinary cooperation in the training of the specialists in the field of Information Technologies
Authors: Butrimienė Edita , Statkevičienė Vida , Marčiulynienė Rita
Published: October 2023

In the article there is analyzed if the transdisciplinary cooperation influences the students’ learning results, and if such cooperation serves for the training of a specialist, who could work in a new environment of knowledge society that motivates a project work in continually variable commands. There is discussed a 3 year-experience of the application of the transdisciplinary cooperation in the training of students in the faculty of information science at Vytautas Magnus University and at Kaunas Institute of Arts, VAA (Vilnius Academy of Fine Arts). There are also investigated some research results of the transdisciplinary cooperation in training as well as the assessment of the students on their own.
Biosensor response to multi-component mixtures statistical analysis and forecasting
Authors: Baronas Romas , Būda Sigitas , Ivanauskas Feliksas , Vaitkus Pranas
Published: October 2023

This paper deals with an analysis of the electrochemical biosensors and their response to multi-component mixtures. The main task is to build a mathematical model for estimation the concentration of each mixture component from the biosensor response data. Two different types of biosensors: amperometric and potenciometric are analysed. Due to high dimensionality of biosensor output data the principal component analysis is applied. Additional multivariate analysis of variance is used to analyze the response sensitivity  of each biosensor type. Finally a concentration estimation model based on ensemble of neural networks is presented.
IMPACT OF INNOVATIVE ICE HOCKEY TRAINING METHODOLOGY ON THE IMPROVEMENT PROCESS
Authors: Krumov I.
Published: October 2023

The main goal of this study is to apply and experiment a methodology for improving tactical training in ice hockey. For the proper conduct of the research and data comparison, two groups- experimental and control one, consisting of twenty children - eight to twelve age ranges, were formed. The research was conducted in 2018-2019 at the ice rink in the Winter Palace of Sports. Measurements including forward sprint 36m, forward sprint 9x6, slalom sprint, and sprint x8 were conducted. The results showed that the improvement in all specialized test indicators of the experimental group was obvious. The research and analysis of innovative training methodology in ice hockey techniques have proven positive changes in young players because it measured their performance before and after implementing the new techniques. The data collected showed improvement in various aspects such as speed, accuracy, and overall performance in game-like situations. Additionally, the specific and task-oriented training approach allowed players to learn and internalize the skills in a more efficient manner, resulting in faster development and improvement. Based on our data, we found that the created innovative training methodology in ice hockey technique offers a significant improvement in the process of motor actions refinement and tactical construction of dynamic situations.
First record of Gargaphia paula Drake, 1939 (Hemiptera, Tingidae) from Colombia, with a new host plant and an updated checklist of Gargaphia species in the country
Authors: Gamboa Jean , Fontecha Carolina , Vergara-Navarro Erika Valentina , Carreño-Guevara Yennifer Andrea , Quiroz John , Serna Francisco
Published: October 2023

Gargaphia paula Drake, 1939 is recorded for the first time for Colombia in two extreme localities, one in the northern region of the country and the other in the southeast. Moreover, it was found on Calopogonium mucunoides Desv. (Fabaceae), a new host plant recorded for the species.
POWER SUPPLY OF RING ANTENNA USING DIRECTIONAL COUPLERS
Authors: Ilnitskyi L. Ya. , Shcherbyna O. A. , Zaliskyi M. Yu. , Mykhalchuk I. I. , Kozhokhina O. V.
Published: October 2023

Context. The circular polarization of radio waves is used in various electronic systems. This includes, for example, space communications stations, some radio relay communication systems, radar stations, data transmission systems and others. The characteristics of radio wave propagation are studied by using electromagnetic waves separated by circular orthogonal polarization in radiomonitoring and radiocontrol systems. Compared to other antenna types, circularly polarized antennas, such as rings, have superior design simplicity and excellent electrodynamics properties.

Objective. The objective of this study is to analyse the characteristics and application of directional microstrip couplers for supplying power to ring antennas.

Method. To better the performance of microstrip ring antennas, the reasons for their limited operating frequency range are analysed. These causes include the frequency-dependent parameters of the coupler, errors in calculating the directional coupler circuit, and radiation from asymmetric strip lines. To understand how supply lines, affect antenna characteristics, correlations between radiation fields determined in both its coordinate system and that of the primary axis are taken into account.

Results. An analysis of the dependence graphs of the main characteristics of ring microstrip antennas with intricate power supply circuits for directional couplers and comparison with similar characteristics for simple circuits revealed that the shape of the radiation pattern in the higher radiation hemisphere became symmetrical about the axis, especially when symmetrically supplying the ring with branch-line couplers. The frequency band has also widened, at which there was an acceptable degree of deviation in the ellipticity coefficient from unity.

Conclusions. The simulation results of microstrip ring antennas with power lines connected to directional couplers of different types showed that supplying the ring antenna with electricity via the directional coupler ensures circular polarization for the emitted electromagnetic waves. Additionally, the range of operating frequencies where there is only a small discrepancy in ellipticity coefficient remains at an acceptable level of –3 dB is quite broad. By utilizing directional branch-line couplers to power a ring antenna, it is possible to simultaneously emit both right and left circularly polarized waves with the same antenna.
MATHEMATICAL MODEL OF THE CURRENT TIME FOR THREE-FRAGMENT RADAR SIGNAL WITH NON-LINEAR FREQUENCY MODULATION
Authors: Kostyria O. O. , Нryzo A. A. , Dodukh O. M. , Narezhnyi O. P. , Fedorov A. V.
Published: October 2023

Context. The authors of the article have developed a new mathematical model that allows taking into account frequency and phase distortions that occur in a three-fragment signal during the transition from one fragment to another, when the rate of frequency modulation of the signal changes. The object of research is the process of formation and processing of radar non-linear frequency modulation signals.

Objective. The purpose of the work is to develop and research a mathematical model of current time for a signal with non-linear frequency modulation, which consists of three linear frequency modulated fragments.

Method. The article provides a theoretical justification of the need to develop a mathematical model in the current time for a three-fragment signal with non-linear frequency modulation, capacity for work of the created model is demonstrated on the example of several radio signals that differ in frequency parameters. With the same signal parameters, the obtained results were compared with the results of the known model, for which known methods of spectral and correlation analysis were used. A distinctive feature of the proposed model is the consideration of jumps in the instantaneous frequency and phase of the signal that occur during the transition from one linear-frequency modulated fragment to the next. Such jump-like changes in frequency and phase in known models of signals with non-linear frequency modulation are not compensated for, which causes distortion of their spectra and an increase the side lobes level of auto-correlation (mutual-correlation) functions.

Results. A comparative check of the developed and known signal models indicates a decrease the side lobes level of the autocorrelation function by 3 dB or more, depending on the given frequency-time parameters.

Conclusions. The application of the proposed mathematical model makes it possible to form and process radar signals, which include three linear-frequency modulated fragments. Compensation of jump-like changes in frequency and phase leads to a decrease in the degree of distortion of the spectrum and, as a result, an increase in its effective width, which ensures a narrowing of the main lobe and a decrease the side lobes level of the auto-correlation function.
RECOGNITION OF REFERENCE SIGNALS AND DETERMINATION OF THEIR WEIGHTING COEFFICIENTS IF AN ADDITIVE INTERFERENCE PRESENTS
Authors: Avramenko V. V. , Bondarenko M. O.
Published: October 2023

Context. The subject matter of the article is the recognition of a reference signal in the presence of additive interference.

Objective. The recognition of the reference signal by the obtained value of its weighting factor in conditions where additive interference is imposed on the spectrum of the reference signal at unknown random frequencies. The task is the development of a method for recognizing a reference signal for the case when the interference consists of an unknown periodic signal that can be represented by a finite sum of basis functions. In addition, interference may also include deterministic signals from a given set with unknown weighting coefficients, which are simultaneously transmitted over the communication channel with the reference signal.

Method. The method of approximating the unknown periodic component of the interference by the sum of basis functions is used. The current number of values of the signal that enters the recognition system depends on the number of basis functions. This signal is the sum of the basis functions and the reference signal with unknown weighting coefficients. To obtain the values of these coefficients, the method based on the properties of the disproportion functions is used. The recognition process is reduced to the calculation of the weight coefficient of the reference signal. If it is zero, it indicates that the reference signal is not part of the signal being analyzed. The recognition system is multi-level. The number of levels depends on the number of basis functions.

Results. The obtained results show that, provided that the reference signal differs by at least one component from the given set of basis functions, the recognition is successful. The given examples show that the system recognizes the reference signal even in conditions where the weighting coefficient of the interference is almost 1000 times greater than the coefficient for the reference signal. The recognition system also works successfully in conditions where the interference includes the sum of deterministic signals from a given set, which are simultaneously transmitted over the communication channel.

Conclusions. The scientific novelty of the obtained results is that a method for recognizing the reference signal has been developed in conditions where only an upper estimate of its maximum frequency is known for the periodic component of the interference. Also, recognition occurs when, in addition to unknown periodic interference, the signals from a given set with unknown weighting coefficients are superimposed on the reference signal. In the process of recognition, in addition to the weighting factor for the reference signal, the factors for the interference components are also obtained.
Approaches to surgical treatment and antibacterial therapy in patients with chronic infection after war injuries
Authors: Trampuz Andrej , Pidgaiska Olga , Filipenko Volodymyr , Romanenko Kostiantyn , Marushchak Olexii
Published: October 2023

Fracture-related infection following orthopedic surgery, especially in cases of war-related trauma, represents a grave complication. The injuries sustained in war often entail severe damage to soft tissues, including significant impairment of vessels, nerves, tendons, muscles, and result in substantial bone defects. Complicating matters further, these infections often involve multidrug-resistant pathogens, making effective treatment a significant challenge. Optimal management of patients with combat-related trauma and signs of infection necessitates specialized care in dedicated centers. The approach to treatment should be guided by a well-defined algorithm that incorporates appropriate surgical interventions alongside systemic and localized antibiotic administration. In instances where chronic infection manifests after war-related injuries and specific causative agents are not definitively identified, initiating empiric therapy is advisable. A combination of meropenem, colistin, and vancomycin can be a suitable choice for initial treatment. Subsequently, once the causative microbes are identified, targeted treatment can be prescribed based on the susceptibility patterns. This article delves into the primary pathogens commonly found in war-related wounds and provides effective antibiotic regimens based on the specific microorganisms. One promising approach for managing severe war injuries is suppressive antibiotic therapy, which enhances the prospects of successful treatment. The comprehensive strategy outlinedhere aims to mitigate the serious risks posed by fracture-related infections in the context of war-induced trauma, ultimately improving patient outcomes and prognosis.
Assessing a relation between composition of luminal and tissue microbiota and cervical intraepithelial changes
Authors: Chechko Svetlana M. , Lyamin Artem V. , Kazakova Anna V. , Yanchenko Anna V. , Sapozhkova Natalya V. , Katorkina Elena S. , Stolbova Marina E.
Published: October 2023

The incidence of cervical cancer (CC) in Russia remains at a high level and ranks fourth among all cancers. CC is preceded by mild, moderate, and severe cervical intraepithelial neoplasia. Human papillomavirus (HPV) is known to be the main cause for its development being responsible for 99% cancer cases. Despite HPV infection, the oncological process occurs only under certain conditions. There are risk factors that indirectly affect the course and emergence of cervical dysplasia. In recent years, the role of cervico-vaginal microbiome for onset and progression of this pathology has been actively discussed. Many studies evidence that dysbiosis along with cervical intraepithelial dysplasia is associated with increased HPV viral load, additionally allowing to clarify a role of specific microorganisms. Despite the knowledge rapidly accumulating about the nature of vaginal microbiome in cervical precancerous processes, the level of its impact on disease course has not been fully investigated and is of great interest. Identification of microorganisms that affect emergence and progression of this pathology will allow to prevent and apply select approach to treatment of dysbiosis. The aim of our study was to identify a relation between cervical tissue and luminal culturome and severe cervical intraepithelial neoplasia. The study evaluated the microbiota of cervical and cervical canal mucosa biopsy in patients with cervical intraepithelial changes. According to the study results, the Streptococcus genus representatives were significantly more frequent in cervical biopsy specimens in severe dysplasia vs. no cervical intraepithelial changes, whereas cervical canal cultures provided comparable data. Bacteria from the Corynebacterium genus were found in cervical biopsy specimens 3 times more often than in smear from the cervical canal mucous membrane. The microbiota from cervical and cervical canal biopsy specimens differ qualitatively and quantitatively particularly regarding prevalence of Streptococcus spp. representatives being more common in patients with cervical dysplasia. Corynebacterium spp. were detected more often in cervical biopsy specimens than in cervical canal smears. Thus, the method of collecting biological material has a great influence on final results.
A clinical case of posttraumatic osteomyelitis associated with kytococcus schroeteri and enterococcus faecalis
Authors: Borisov Sergey D. , Karimov Ilshat F. , Plotnikov Andrey O. , Inchagova Ksenia S. , Pankov Alexandr S. , Danshin Dmitry P.
Published: October 2023

Introduction. In most cases, osteomyelitis is the monomicrobial disease caused by various Gram-positive bacteria. However, previous injury may contribute to formation of a microbial association. Presence of two or more pathogens in the infectious focus can markedly complicate the disease clinical picture.Kytococcus schroeteriis one of the rarest pathogens found in clinical samples. Slightly more than twenty described cases related to such infection have been recorded globally. The aim of the research was to analyse the first identified clinical case of post-traumatic osteomyelitis associated withKytococcus schroeteriandEnterococcus faecalis.Materials and methods.The bacterial cultures were obtained from the wound discharge of the patient’s shin by the cultural method. Identification of the bacterial cultures was performed using a VITEK MS mass spectrometer followed by 16S rRNA gene sequencing. The morphological, tinctorial and biochemical features of the cultures obtained were established, and their antibacterial resistance was also determined.Results. The clinical case was associated with the diagnosis “Chronic post-traumatic osteomyelitis of the left shin”. Two types of microorganisms were isolated from the wound discharge, and identified asKytococcus schroeteriandEnterococcus faecalisusing the MALDI-ToF mass spectrometry method. 16S rRNA gene sequencing from the isolated bacterial cultures confirmed the MALDI-ToF mass spectrometry data. Antimicrobial susceptibility for this microorganism was determined according to the criteria for the staphylococcal group according to the clinical guide “Determination of sensitivity to antimicrobial drugs (rev. 2021)”.Conclusion. For the first time, the mixed infection caused by two Gram-positive bacteriaK. schroeteriandE. faecalishas been described. Such associations can enhance the pathogenic effects of each other bacterium, which may contribute to transition of the infection to a chronic form with a low probability of positive cultural test.K. schroeteriis a representative of the normal skin microbiota, but this microorganism is able to cause various infections. TheK. schroeterispecies should be differentiated from other representatives of the orderMicrococcales. At cultural examination, resistance to oxacillin is a suspicious sign indicating that the bacterial culture might be potentially assigned toK. schroeterispecies. Due to the unavailability of current biochemical tests for differentiationK. schroeterifrom related taxa, reliable identification of this species is recommended using MALDI-ToF mass spectrometry or 16S rRNA gene sequencing.
Virus neutralizing antibodies in pseudovirus particle neutralization reaction as a bioanalytical part of a Salnavac® vaccine clinical trial
Authors: Zuev Evgeniy V. , Markova Oksana Anatolievna , Kulemzin Sergey V. , Poteryaev Dmitry A. , Litvinova Natalya A. , Korotkevich Ilya A. , Grigiryeva Taisiya V. , Khamitov Ravil A.
Published: October 2023

Introduction. The SARS-CoV-2 coronavirus pandemic has been a major challenge for all areas of medical science, causing a surge of new developments in various fields ranging from diagnostic techniques to therapeutic and preventive approaches. Intranasal vaccination is an innovative approach to immunization against SARS-CoV-2, which has attracted the attention of many drug developers. Dynamics of blood virus-neutralizing antibodies (VNAs) in recovered COVID-19 patients or vaccinated healthy volunteers is one of the objective parameters for assessing vaccine immunological efficacy, which requires high standards of bioanalytical techniques within the framework of clinical trials. Immunogenicity data on the two-component Salnavak®(intranasal) and Gam-COVID-Vac®(intramuscular) vaccination obtained in randomized double-blind multicenter phase 3 clinical trial interim analysis are presented. The objective of the study was to assess immunogenicity of intranasal and intramuscular vaccination against COVID-19 using a neutralization reaction with pseudoviral particles and HEK293T-hACE2 cell culture.Materials and methods.A total of 137 healthy volunteers with a baseline anti-RBD IgG level not exceeding 100 BAU/ml received immunization by a two-component (Ad26 and Ad5 based) intranasal or intramuscular vaccine administered on day 1 and day 21. Immunogenicity level based on VNA quantitative analysis using a neutralization reaction with pseudoviral particles and a HEK293T-hACE2 cell culture as well as to SARS-CoV-2 S-protein receptor-binding domain (anti-RBD) IgG antibodies on days 21 and 42 after administration of component I was assessed.Results.The geometric mean VNA titer against SARS-CoV-2 on day 42 was 238.34±3.93 and 616.94±3.73 in the Salnavac®and Gam-COVID-Vac®groups, respectively. Trial data shows sufficient immunological efficacy of both intramuscular and intranasal vaccines based on a high protection level at VNA titer of more than 100 while using the pseudoviral neutralization method. The geometric mean of the anti-RBD IgG level by day 42 was 131.22±3.91 and 782.03±3.04 in the Salnavac®and Gam-COVID-Vak®groups, respectively. A direct moderate correlation was shown between VHA and anti-RBD IgG.Conclusion. Neutralization reaction using pseudoviral particles was successfully validated and used to determine the VNA titer during clinical trial. Trial interim data revealed that intranasal vaccine Salnavac®vs intramuscular vaccine Gam-COVID-Vak®resulted in lower but sufficient stringency of humoral immunity.
Design Thinking aplicado como metodologia para a solução de problemas no ensino de Química: um estudo de caso a partir de uma problemática ambiental
Authors: Silva-Neto Sebastião Luiz da , Leite Bruno Silva
Published: October 2023

Resumo O Design Thinking (DT) é uma metodologia ativa que auxilia no processo de construção do conhecimento, em que o estudante se torna ativo e o professor atua como mediador no processo de ensino e aprendizagem. Esta pesquisa objetivou analisar, por meio do DT como metodologia de ensino, as ações de estudantes do curso de licenciatura em Química de uma Universidade Federal, na sugestão de protótipos para resolução de uma problemática ambiental. Por meio de uma abordagem qualitativa, a partir de um estudo de caso, a pesquisa foi realizada em três momentos, a fim de descrever o caminho percorrido pelos estudantes para a resolução dos problemas ambientais. Para análise dos dados, recorreu-se a elementos da Análise Textual Discursiva. Os resultados demonstram que, durante o DT, os estudantes fizeram uso dos conhecimentos prévios, experiências pessoais, culturais, econômicas e sociais, além do conhecimento químico (em uma perspectiva ambiental), para a resolução do problema proposto.
Representações multimodais em aulas de Ciências da Educação Básica: o papel das representações informais
Authors: Botelho Maria Luiza Silva Tupy , Quadros Ana Luiza de
Published: October 2023

Resumo A Ciência tem se utilizado de diferentes representações no processo de produção e comunicação do conhecimento. No ensino de Ciências as representações são parte do processo cognitivo de aprendizagem de conceitos. Neste trabalho analisamos o papel das representações informais - balões de festa e representação com o corpo - na construção de significados, nas aulas de dois professores. Para aproximar o ensino de Ciências de práticas científicas autênticas, estudantes foram envolvidos na proposição, justificação, negociação e reelaboração de representações, processo esse denominado representações multimodais. Na análise segmentamos as aulas em episódios e transcrevemos aqueles em que as representações informais foram usadas. Observamos que os estudantes perceberam aspectos da representação que não haviam percebido com os desenhos, o que lhes permitiu tanto a reelaboração do desenho quanto um entendimento conceitual mais amplo. Isso traz implicações para o campo, uma vez que o ato de representar não pode ser considerado apenas um recurso periférico.
VARIATION AND CHANGE IN THE HISTORY OF THE STRESSED MID VOWELS OF THE PORTUGUESE LANGUAGE: AN INTERPRETATION OF THE RHYMES OF THE POETRY FROM THE PAST
Authors: FONTE Juliana Simões , MASSINI-CAGLIARI Gladis
Published: October 2023

ABSTRACT This research aims to investigate the pronunciation of the stressed mid vowels in Portuguese of the 13th, 15th, 16th and 17th centuries, through the observation of the rhymes of the poetry from that period. The corpora comprise the Cantigas de Santa Maria by Alfonso X, the Cancioneiro Geral by Garcia de Resende, Os Lusíadas by Camões and the sonnets by Gregório de Matos. Knowing that the ancient writing, like the present, had only two graphemes to represent phonemes concerning stressed mid vowels of Portuguese, the possibilities and impossibilities of rhyme between mid vowels represented by identical graphemes were evaluated in this work. The results of this research suggest that there was constant phonetic variation in the speech of the stressed mid vowels of the Portuguese language, between the 15th and 17th centuries (at least), and that, in some cases, this variation resulted in a change, throughout the history of the language.
DIVULGAÇÃO CIENTÍFICA E DIREITOS ANIMAIS PARA A PRIMEIRA INFÂNCIA: PROBLEMATIZANDO OS AQUÁRIOS ATRAVÉS DE PRÁTICAS LÚDICAS EM UMA ESCOLA DE EDUCAÇÃO INFANTIL
Authors: Vizachri Tânia Regina , Piassi Luís Paulo de Carvalho , Giordan Marcelo , Braga Adriana Regina
Published: October 2023

RESUMO: Neste estudo, exploramos como crianças de 4 a 5 anos de idade abordaram os valores atribuídos ao habitat do tubarão. A sequência interativa examinada faz parte de um estudo de caso realizado em uma turma de uma EMEI no município de São Paulo, localizada em uma região periférica e vulnerável, composta por 32 crianças. Para compreender o contexto, elaboramos um mapa episódico e aplicamos as categorias de Lemke (1990) e Mortimer e Scott (2003) no estudo do padrão temático. Nosso objetivo foi verificar até que ponto a interação estabeleceu um espaço dialógico de escuta para as crianças, essencial para o desenvolvimento do pensamento crítico. Os resultados indicam que, desde cedo, as crianças apresentam visões socioculturais sobre o habitat do tubarão. No entanto, através de atividades lúdicas que incentivam a formulação de perguntas e a consideração de diferentes perspectivas, foi possível estimular o pensamento crítico e a reflexão ética das crianças sobre esse ambiente específico.
Can golden mussel shell be an alternative to limestone in soil correction?
Authors: Boni Thaís Soto , Maltoni Kátia Luciene , Souza Loiane Fernanda Romão de , Cassiolato Ana Maria Rodrigues
Published: October 2023

ABSTRACT Golden mussel is an invasive species in South America that causes environmental and economic damage due to the formation of large colonies without natural predators. This study aimed to test the agricultural use of golden mussel shell as a limestone substitute, as the shell is rich in calcium carbonate. The experiment was carried out in pots, with eight treatments (sandy soil; clay soil; sandy soil + 1.0 Mg ha−1 of limestone; clay soil + 1.0 Mg ha−1 of limestone; sandy soil + 1.0 Mg ha−1 of fresh shell; clay soil + 1.0 Mg ha−1 of fresh shell; sandy soil + 1.0 Mg ha−1 of calcined shell; clay soil + 1.0 Mg ha−1 of calcined shell), in addition to the application of the following fresh and calcined shell doses: 0, 1.0, 1.5 and 2.0 Mg ha−1. Rice was cultivated in all treatments, and the soil fertility and rice shoot and root dry masses were evaluated. The shell provided good chemical conditions to the soils and raised their pH and phosphorus and calcium contents. The agricultural use of golden mussel shell showed to be efficient for soil correction and can be considered an alternative to limestone.
AUTISM, NEURODIVERSITY AND STIGMA: POLITICAL AND INCLUSIVE PERSPECTIVES
Authors: Araujo Ana Gabriela Rocha , Silva Mônia Aparecida da , Zanon Regina Basso
Published: October 2023

ABSTRACT Movements of people with Autism Spectrum Disorder (ASD) and their families have been increasing and gaining strength in Brazil and worldwide. The neurodiversity movement understands that autism is part of a person’s personality, not a disease that must be cured. The study addresses the neurodiversity movement in the context of ASD, discussing its relations with scientific evidence. From a theoretical study, we saw that the medical model still seems to be the most adopted in Brazilian research about inclusion, which may influence stigma and negative and distorted views about the differences presented by people with ASD. Among other aspects, the importance of the participation of people with ASD and/or their family members in the development of research related to them is highlighted, seeking to promote scientific and academic practices that listen, respect and be inspired by neurodiversity.
UTP23 Is a Promising Prognostic Biomarker and Is Associated with Immune Infiltration in Breast Cancer
Authors: Li Jindong , Xie Siman , Zhang Benteng , He Weiping , Zhang Yan , Wang Jun , Yang Li
Published: October 2023

Breast cancer is one of the malignant tumors with a high incidence and mortality rate among women worldwide, and its prevalence is increasing year by year, posing a serious health risk to women. UTP23 (UTP23 Small Subunit Processome Component) is a nucleolar protein that is essential for ribosome production. As we all know, disruption of ribosome structure and function results in improper protein function, affecting the body's normal physiological processes and promoting cancer growth. However, little research has shown a connection between UTP23 and cancer. We analyzed the mRNA expression of UTP23 in normal tissue and breast cancer using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database, and the protein expression of UTP23 using The Human Protein Atlas (HPA) database. Next, we examined the relationship between UTP23 high expression and Overall Survival (OS) using Kaplan-Meier Plotters and enriched 980 differentially expressed genes in UTP23 high and low expression samples using GO/KEGG and GSEA to identify potential biological functions of UTP23 and signaling pathways that it might influence. Finally, we also investigated the relationship between UTP23 and immune infiltration and examined the effect of UTP23 on the proliferation of human breast cancer cell lines by knocking down UTP23. We found that UTP23 levels in breast cancer patient samples were noticeably greater than those in healthy individuals and that high UTP23 levels were strongly linked with poor prognoses (P = 0.008). Functional enrichment analysis revealed that UTP23 expression was connected to the humoral immune response. Besides, UTP23 expression was found to be positively correlated with immune cell infiltration. Furthermore, UTP23 knockdown has been shown to inhibit the proliferation of human breast cancer cells MDA-MB-231 and HCC-1806. Taken together, our study demonstrated that UTP23 is a promising target in detecting and treating breast cancer and is intimately linked to immune infiltration.
Convulsions associated with gastroenteritis in the spectrum of benign focal epilepsies in infancy: 30 cases including four cases with ictal EEG recording
Authors: Cusmai Raffaella , Jocic‐Jakubi Bosanka , Cantonetti Laura , Japaridze Natia , Vigevano Federico
Published: October 2023

Benign convulsions associated with gastroenteritis are now recognized as a clinical entity, characterized by an acute cluster of afebrile seizures during an episode of mild diarrhoea with excellent prognosis. We observed 30 children who each experienced at least two seizures associated with mild gastroenteritis. The inclusion criteria were: afebrile seizures during gastroenteritis, dehydration at ≤ 5%, normal neurological findings, normal psychomotor development and no underlying pathology according to laboratory and neuroimaging studies. Mean age was 21 months (range: 6‐38). Familial history for epilepsy was positive in 3/30 (10%) and for febrile convulsions in 11/30 (36.6%). Seizure onset was mainly on the third day of gastroenteritis. Seizures were described as generalised by parents in 16/30 patients (53.3%). We directly observed seizures in 14/30 patients (47.7%), and the semiology was partial with secondary generalisation. Focal onset was confirmed in two patients by EEG and in two patients by video‐EEG recording. Twenty of 30 patients (66.6%) received antiepileptic drugs during the acute phase. Ten patients (33.3%) received no treatment. During follow‐up (mean duration: 53 months), one patient had an isolated afebrile seizure and two others a febrile seizure. At the end of follow‐up, antiepileptic treatment was withdrawn for all but two patients. None developed epilepsy. Although the pathogenesis of this clinical entity is unknown, we hypothesize that mild gastroenteritis may provoke a transient brain dysfunction which in turn provokes seizures in children with genetically determined susceptibility. [Published with video sequences]
Unilateral opercular lesion and eating‐induced seizures
Authors: Manyam Siresha Chaluvadi , Kung Doris H. , Rhodes Lisa B. , Newmark Michael E. , Friedman David E.
Published: October 2023

Eating‐induced seizures are an uncommon presentation of reflex epilepsy, a condition characterized by seizures provoked by specific stimuli. Most reports have identified aetiology associated with malformations of cortical developmental, hypoxic brain injury, previous meningoencephalitis or static encephalopathy. We present a patient with eating‐induced reflex seizures, which began several years after treatment for an opercular primitive neuroectodermal tumour (PNET), and who subsequently underwent in‐depth clinical and video‐EEG analysis for her seizures. This patient noted rapid improvement with decreased frequency of seizure activity after treatment with valproic acid. We discuss the aetiology of reflex epilepsy, the anatomical basis of eating‐induced epilepsy, and review the current literature. [Published with video sequences]
The Effect of Local Government Supports on Export of SMEs: Focused on 4th Industrial Revolution Firms
Authors:   The Korea International Trade Research Institute, Huang Yi , Kim Sung-Hwan , Park Sung-Su
Published: October 2023

Purpose - This research aims to analyze the impact of local government support on the exports of small and medium-sized enterprises (SMEs), with a particular focus on 4th industrial revolution firms.
Design/Methodology/Approach - In this study, we use corporate information obtained from the Korea Enterprise Data (KED) database from 2015 to 2020 for firms located in Gyeongsangbuk-do. We conducted a fixed-effects panel regression analysis to diagnose the effect of local government support on the exports of firms. A total of 41,153 firm-year data from 10,840 firms were used in the analysis.
Findings - The results show that local government support for SMEs have a statistically significant negative effect on the exports of 4th industrial revolution firms in the region. However, scientific and technological support by the local government have a positive effect on the exports of the 4th industrial revolution firms. Their yearly effects are usually reversed over time after budget support. Among local government budgets, only the science and technology support budget and the education support budget positively impact export performance, and small and medium-sized enterprises and social welfare support budgets negatively affect exports. In addition, the result of R&D investment in 4th industrial companies is not statistically significant.
Research Implications - With different effects on firm exports, governments, either local or central, shouold consider the short-term and long-term effects in providing subsidies and support.
Clustering and Determinant Analysis of Corporate Environmental Management Attitudes: Unsupervised Learning Techniques
Authors:   The Korea International Trade Research Institute, An Ji-Young
Published: October 2023

Purpose - The main objective of this study is to cluster companies based on environmental management attitudes using various indicators, and analyze differences in activities among the clusters to gain insights into environmental management performance.
Design/Methodology/Approach - The study encompasses panel data from 12 countries, 22 industry sectors, and 3,402 companies from 2013 to 2022. This study uses both machine learning methodology and conventional econometric methodology. The primary methodology involves using an unsupervised learning technique, K-means clustering, to categorize environmental management attitudes. Subsequently, Kernel Density estimation and Multinomial Logistic Regression were employed to conduct comparative analyses of environmental performance indicators within each cluster and identify the determinants shaping environmental management attitudes.
Findings - The research revealed the existence of five distinct clusters of companies with varying environmental management attitudes. Through Multinomial Logistics Regression, it identified that variables such as greenhouse gas emissions, energy consumption, total assets, return on assets, and other significantly influence environmental management attitudes.
Research Implications - By uncovering common characteristics and determinants within major countries and industries, this study offers valuable insights in promoting domestic environmental management in the context of rapidly changing international trade and investment environments. The findings provide policy implications and suggestions for fostering proactive environmental management practices among domestic companies.
Taxonomical and chorological notes 17 (178–183)
Authors: Rigó Attila , Döme Bernadett , Dudáš Matej , Karikás Maja , Lőkös László , Pifkó Dániel , Varga Nóra , Farkas Edit
Published: October 2023

Floristical records of two lichen-forming fungi and four flowering plants are presented from Hungary. The rare lichen-species Gyalecta fagicola now is known from 7 localities in Hungary with 5 new records. Polyblastia philaea a terricolous lichen species is new to Hungary. The first confirmed record (Szentendre) of the annual weed Adonis annua, a new occurrence (Bodrog bank, Sárospatak) of Cyperus michelianus in the upper Tisza region, and a new locality of the alien weed Rapistrum rugosum from Szentendre (Bizottság-liget) are reported. The naturalised weed Polycarpon tetraphyllum has a stable, spreading population in Budapest with several thousands of individuals.
On the Tang Dynasty's Rule over the Nomadic People in Ancient Inner Mongolia: With Jimi Prefecture as the Core
Authors:   The Korean Association for Mongolian Studies, Wang Li-juan
Published: October 2023

During the Tang Dynasty, northern nomadic nationalities such as Turkic, Huihe, Khitan, Xi, and Shiwei were active in the Inner Mongolia region, and the Tang Dynasty established different degrees of contact with these ethnic groups.The Tang Dynasty set up Yunzhong, Chanyu, Yanran, Hanhai, Anbei Duhufu and Songmo, Raole, Shiwei and other Dudufu to achieve the fettered rule of these ethnic groups.The establishment of the prefecture was the embodiment of the border governance and ethnic policy of the Tang Dynasty, which contributed to the stability of the Tang Dynasty's frontier,promoted the development of the Inner Mongolia region and the exchanges, exchanges and blending of various ethnic groups in the fields of political, economic, cultural and so on.
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Acquisition by Data-Independent Acquisition (DIA) on an Orbitrap Eclipse Tribrid Mass Spectrometer v1
Authors: not provided JBons , P Rose J , A Watson M , Schilling B
Published: October 2023

Proteolytic peptide measurement using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Acquisition by Data-Independent Acquisition (DIA) on an Orbitrap Eclipse Tribrid Mass Spectrometer for peptide/protein identification and quantification.
Tissue Protein Extraction: Tissue Homogenization using Urea-based Buffer and Bead Mill Homogenizers v1
Authors: not provided JBons
Published: October 2023

Tissue homogenization to isolate protein in preparation for downstream proteomic profiling.
Cell Lysate Preparation & Immunoblotting Protocols v1
Authors: not provided aasirvatham
Published: October 2023

The purpose of this experiment is to investigate the effects of forskolin-mediated cAMP activation on the viability of LPS-treated Schwann cells. The immortalized rat RT4-D6P2T cell line (ATCC #CRL-2768) was cultured and received one of the following treatments: 0.1, 1, or 10 μg/mL of LPS, in N2 media (control) or N2 media supplemented with 2 μM of forskolin, for 1, 3, 12, or 24 hours. Cell lysates were prepared, and immunoblotting was performed to quantify changes in NF-κB, TNF-ɑ, AKAP95, and cyclin D3 expression in response to the different treatment combinations.
Immunoblot v1
Authors: Peñuelas Núria
Published: October 2023

Immunoblot of rodent brain regions
The effectiveness of Jigsaw cooperative learning model in developing students’ oral communication skills and cognitive learning outcomes
Authors: Priadi Median Agus , Jalmo Tri , Sikumbang Darlen , Meilinda Kartika
Published: October 2023

This study aims to determine the effectiveness of Jigsaw cooperative learning model in developing communication skills and cognitive learning outcomes of students. This research using the one group pretest-posttest design. The sample is the students of class X MIA 1 who were selected through purposive sampling technique. Data on oral communication skills were carried out by self-assessment, peer assessment and observation which were analyzed using a Likert scale. Meanwhile, data on cognitive learning outcomes were obtained through pretest and posttest scores, then analyzed by performing the n-gain score test. The research data shows that students have oral communication skills in the "high" category, which is as much as 77%. The highest verbal communication skills of students are found in the indicator of delivering detailed information with the "very high" category, while the lowest communication skills are found in the indicator of giving questions in the "enough" category. Then for learning outcomes, statistically student learning outcomes showed a significant difference between initial understanding (pretest) and understanding after learning (posttest) indicated by a significant N-gain value of 0.73 with high criteria. Thus, it can be concluded that the Jigsaw cooperative learning model is effective in developing communication skills and cognitive learning outcomes of students.
Development of STEM-based interactive e-module on ecology topic for senior high schools’ student
Authors: Fauzi Rizqi , Hayya Adieba Warda
Published: October 2023

The quality of education must always be improved to get maximum results. One way to improve it is by developing learning media. Based on the results of field observations in this study at MA Raudlatus Shibyan, the use of learning media was still not optimal. For this reason, researchers developed an Interactive E-module product containing Ecology topic in Biology Learning as a supplement to learning. An interactive e-module is an electronic module that contains materials, videos, audio, animations, quizzes, and evaluations, which allow students to study independently. This descriptive research consists of three main stages, include Planning, Project, Evaluation (PPE). The interactive e-module developed was compiled using the Canva web, then the file was saved as a PDF, after which editing was continued using the Flip PDF Professional application. The product was then validated by a material expert and got a percentage score of 98.12% in the "Very Feasible" category. Validation by media experts got a score of 93.25% in the "Very Decent" category. After going through validation and revision according to the suggestions, the product was tested on Biology teachers and students of class X science. The score from the teacher's assessment is 79.76% in the "Eligible" category. Assessment from students got a score of 80% in the "Eligible" category. Based on the results obtained from product validation and testing, the interactive E-module developed by the researcher was feasible to be used in the field in terms of valid and practical feasibility.
Development of student worksheet on growth and development of drought-stressed plants
Authors: Mamonto Saifullah , Kandowangko Novri Youla , Yusuf Frida Maryati
Published: October 2023

This study aimed to determine the validity, practicality, and effectiveness of developing student worksheets on the growth and development of drought-stressed plant material. The subjects of this study were students of 12th grade Science 1 of Senior High School 1 Tapa. The worksheet development used the ADDIE development model. The instruments used were Validation Sheets, Learning Implementation, Student Activities, Student Response Questionnaires, and THB questions. Student worksheet based on drought stressed plant study of growth and development material has met the valid, practical and effective categories. Based on the results obtained from the validation and trial of products on a limited scale, the development of student worksheets on the growth and development of drought-stressed plant material is suitable to be used for learning, as well as practical, and can improve student learning outcomes.
Validity analysis of digital module based on generic science skills on environmental pollution topic for 10th grade students
Authors: Utami Nurul Hidayati , Kapsul Kapsul , Putra Aminuddin Prahatama , Jannah Jannah
Published: October 2023

The development of digital modules based on interesting generic science skills can be integrated in biology learning. Generic science skills (GSS) are related to skills through application and biological context. The factual and procedural knowledge obtained by students can be explored in the environmental pollution material because of various concepts and solve various real problems. This research is a descriptive study that explains the stages of digital module validation analysis based on generic science skills on environmental pollution for grade 10 high school students. Digital module validation test refers to five aspects such as (1) suitability of material, (2) presentation, (3) language, (4) generic science skills, and (5) visual/appearance. The subjects in the research at the initial review stage were three validators consisting of practitioners who are experts in learning biology and learning media. Based on the results of the study, it is known that the module developed as a whole is very valid in reference to aspects, namely the feasibility of the content aspect, the feasibility of the presentation aspect, the feasibility of the language aspect, the feasibility of the GSS aspect and the appearance, this shows that the digital module produced can be continued at the next stage.