bluuebunny/crossref_metadata_2025 huggingface dataset:
Fasting Normoglycemia after Intravenous Thrombolysis Predicts Favorable Long-Term Outcome in Non-Diabetic Patients with Acute Ischemic Stroke
Authors: Wnuk Marcin , Derbisz Justyna , Drabik Leszek , Malecki Maciej , Slowik Agnieszka
Published: July 2021

Background: Only a few studies evaluated the role of fasting glucose levels after intravenous thrombolysis (IVT) in patients with acute ischemic stroke (AIS). Importantly, formal analysis concerning the prognostic role of fasting glucose levels in these patients with and without diabetes mellitus (DM) was not performed. Therefore, we assessed whether fasting normoglycemia (FNG) next morning after AIS treated with IVT was associated with 90-day functional outcome in diabetic and non-diabetic patients. Methods: We retrospectively analyzed 362 AIS patients treated with IVT at The University Hospital in Krakow. FNG was defined as glucose below 5.5 mmol/L. A favorable outcome was defined as modified Rankin score (mRS) of 0–2 at day 90 after AIS onset. Results: At 3-month follow-up, FNG was associated with favorable outcome (87.5% vs. 60.8%, p < 0.001) and decreased risk of death (3.1% vs. 18.1%, p = 0.002). Independent predictors of a favorable outcome for the whole group were: younger age (HR 0.92, 95%CI 0.89–0.95), lower NIHSS score after IVT (HR 0.70, 95%CI 0.65–0.76), lower maximal systolic blood pressure within 24 h after IVT (HR 0.92, 95%CI 0.89–0.95) and FNG (HR 4.12, 95%CI 1.38–12.35). Association between FNG and mortality was found in univariable (HR 1.47, 95%CI 0.04–0.62) but not in multivariable analysis (HR 0.23, 95%CI 0.03–1.81). In subgroup analyses, FNG was an independent predictor of favorable outcome (HR 5.96, 95%CI 1.42–25.1) only in patients without DM. Conclusions: FNG next morning after IVT is an independent protective factor for a favorable long-term outcome in non-diabetic AIS patients.
In Vitro Evaluation of the Antiviral Activity of Methylene Blue Alone or in Combination against SARS-CoV-2
Authors: Gendrot Mathieu , Jardot Priscilla , Delandre Océane , Boxberger Manon , Andreani Julien , Duflot Isabelle , Le Bideau Marion , Mosnier Joel , Fonta Isabelle , Hutter Sébastien , La Scola Bernard , Pradines Bruno
Published: July 2021

A new severe acute respiratory syndrome coronavirus (SARS-CoV-2) causing coronavirus diseases 2019 (COVID-19), which emerged in Wuhan, China in December 2019, has spread worldwide. Currently, very few treatments are officially recommended against SARS-CoV-2. Identifying effective, low-cost antiviral drugs with limited side effects that are affordable immediately is urgently needed. Methylene blue, a synthesized thiazine dye, may be a potential antiviral drug. Antiviral activity of methylene blue used alone or in combination with several antimalarial drugs or remdesivir was assessed against infected Vero E6 cells infected with two clinically isolated SARS-CoV-2 strains (IHUMI-3 and IHUMI-6). Effects both on viral entry in the cell and on post-entry were also investigated. After 48 h post-infection, the viral replication was estimated by RT-PCR. The median effective concentration (EC50) and 90% effective concentration (EC90) of methylene blue against IHUMI-3 were 0.41 ± 0.34 µM and 1.85 ± 1.41 µM, respectively; 1.06 ± 0.46 µM and 5.68 ± 1.83 µM against IHUMI-6. Methylene blue interacted at both entry and post-entry stages of SARS-CoV-2 infection in Vero E6 cells as retrieved for hydroxychloroquine. The effects of methylene blue were additive with those of quinine, mefloquine and pyronaridine. The combinations of methylene blue with chloroquine, hydroxychloroquine, desethylamodiaquine, piperaquine, lumefantrine, ferroquine, dihydroartemisinin and remdesivir were antagonist. These results support the potential interest of methylene blue to treat COVID-19.
Ultrasound Evaluation of the Rectus Femoris for Sarcopenia in Patients with Early Subacute Stroke
Authors: Choi Yongmin , Im Sun , Park Geun-Young
Published: July 2021

We investigated the ultrasound characteristics of the rectus femoris for sarcopenia detected by dual-energy X-ray absorptiometry (DEXA) in the early subacute stroke phase. Physical features (age, sex, body mass index, and circumference of thigh) and performances (modified Barthel index in Korean, functional ambulation categories, and mini-mental state examination in Korean) were measured. The thickness of the fat layer, the thickness of the rectus femoris (TRF), echo intensity (EI), EI to TRF ratio, and strain ratio of elastography (SRE) were measured by ultrasound in 30 patients with first-ever stroke (male: n = 20). Appendicular lean body mass was measured by DEXA. Sarcopenia was defined according to the Foundation for the National Institutes of Health Sarcopenia Project. In total, 14 patients were in the sarcopenia group, and 16 were in the non-sarcopenia group. Clinical characteristics were similar between the two groups. In the sarcopenia group, TRF was significantly decreased in the paretic (p < 0.026) and non-paretic sides (p < 0.01), and the EI to TRF ratio on the paretic side was significantly increased (p < 0.049). Multivariate binary logistic regression showed that TRF on the non-paretic side was independently and significantly associated with sarcopenia (OR = 0.616, 95% CI: 0.381–0.996). The EI and SRE were not significant between the two groups. In the early subacute stroke phase, TRF on the non-paretic side is a key factor for quantitative evaluation of sarcopenia, and the EI to TRF ratio on the paretic side is also a meaningful qualitative evaluation of sarcopenia.
Clinical Outcomes and Joint Stability after Lateralized Reverse Total Shoulder Arthroplasty with and without Subscapularis Repair: A Meta-Analysis
Authors: Corona Katia , Cerciello Simone , Ciolli Gianluca , Proietti Lorenzo , D'Ambrosi Riccardo , Braile Adriano , Toro Giuseppe , Romano Alfonso Maria , Ascione Francesco
Published: July 2021

Introduction: Subscapularis tendon repair in reverse total shoulder arthroplasty represents a potentially modifiable risk factor for dislocation, and its role continues to be debated. The purpose of the present meta-analysis was to compare the outcomes of the primary lateralized RSAs with and without subscapularis repair in terms of range of motion, clinical outcomes, dislocations, and complications rate. Materials and Methods: A systematic literature search in MEDLINE (Pubmed), Embase, and the Cochrane Central Register of Controlled Trials database was carried up to December 2020. A data extraction form was developed to collect select data from the included studies. The methodological quality was assessed using a Methodological Index for Nonrandomized Studies (MINORS) score. Statistical analysis was performed with Review Manager (Version 5.4, The Cochrane Collaboration). Results: A total of four comparative studies involving 978 patients were included. In the pooled analysis, the reinsertion of the subscapularis yielded better functional outcomes in terms of the constant (P < 0.00001) and ASES (P = 0.002) scores. The forward elevation, external rotation at 0°, internal rotation, and dislocation rates were comparable between the two groups (P = n.s.), while statistically increased abduction was observed in those patients who did not have their subscapularis repaired (P < 0.00001). Conclusion: The results of the present findings suggest that it seems reasonable to reinsert the subscapularis whenever it is present, in good tissue conditions, and with no evidence of fatty degeneration of its muscle belly. Level of evidence: Level III meta-analysis
High WHSC1L1 Expression Reduces Survival Rates in Operated Breast Cancer Patients with Decreased CD8+ T Cells: Machine Learning Approach
Authors: Kim Hyung-Suk , Min Kyueng-Whan , Kim Dong-Hoon , Son Byoung-Kwan , Kwon Mi-Jung , Hong Sang-Mo
Published: July 2021

Nuclear receptor-binding SET domain protein (NSD), a histone methyltransferase, is known to play an important role in cancer pathogenesis. The WHSC1L1 (Wolf-Hirschhorn syndrome candidate 1-like 1) gene, encoding NSD3, is highly expressed in breast cancer, but its role in the development of breast cancer is still unknown. The purpose of this study was to analyze the survival rates and immune responses of breast cancer patients with high WHSC1L1 expression and to validate the results using gradient boosting machine (GBM) in breast cancer. We investigated the clinicopathologic parameters, proportions of immune cells, pathway networks and in vitro drug responses according to WHSC1L1 expression in 456, 1500 and 776 breast cancer patients from the Hanyang University Guri Hospital, METABRIC and TCGA, respectively. High WHSC1L1 expression was associated with poor prognosis, decreased CD8+ T cells and high CD274 expression (encoding PD-L1). In the pathway networks, WHSC1L1 was indirectly linked to the regulation of the lymphocyte apoptotic process. The GBM model with WHSC1L1 showed improved prognostic performance compared with the model without WHSC1L1. We found that VX-11e, CZC24832, LY2109761, oxaliplatin and erlotinib were effective in inhibiting breast cancer cell lines with high WHSC1L1 expression. High WHSC1L1 expression could play potential roles in the progression of breast cancer and targeting WHSC1L1 could be a potential strategy for the treatment of breast cancer.
Appetite and Nutritional Status as Potential Management Targets in Patients with Heart Failure with Reduced Ejection Fraction—The Relationship between Echocardiographic and Biochemical Parameters and Appetite
Authors: Kaluzna-Oleksy Marta , Sawczak Filip , Kukfisz Agata , Szczechla Magdalena , Krysztofiak Helena , Wleklik Marta , Przytarska Katarzyna , Migaj Jacek , Dudek Magdalena , Straburzyńska-Migaj Ewa , Uchmanowicz Izabella
Published: July 2021

This study aimed to investigate the role of appetite loss and malnutrition in patients with heart failure with reduced ejection fraction (HFrEF). In this prospective, observational, single-center study, we enrolled 120 consecutive adults with HFrEF. We analyzed the selected clinical, echocardiographic, and biochemical parameters. Appetite loss and malnutrition were assessed by CNAQ (Council on Nutrition Appetite Questionnaire) and MNA (Mini Nutritional Assessment)/GNRI (Geriatric Nutritional Risk Index) questionnaires, respectively.Most patients were men (81.7%), mean age was 55.1 ± 11.3 years, and mean left ventricular ejection fraction was 23.9 ± 8.0%. The mean CNAQ score was 28.8 ± 3.9, mean MNA—23.1 ± 2.6, and mean GNRI—113.0 ± 12.3. Based on ROC curves, we showed that a sodium concentration <138 mmol/L had the greatest discriminating power for diagnosing impaired nutritional status (MNA ≤ 23.5) with a sensitivity of 54.5% and specificity of 77.8%. The threshold of HDL <0.97 mmol/L characterized 40.7% sensitivity and 86% specificity, B-type natriuretic peptide >738.6 pg/dL had 48.5% sensitivity and 80.8% specificity, high-sensitivity C-reactive protein >1.8 mg/L had 94.9% sensitivity and 42.9% specificity, and bilirubin >15 µmol/L had 78.2% sensitivity and 56.9% specificity. Nutritional status and appetite assessed by MNA/GNRI and CNAQ questionnaires showed poor correlations with other findings in HFrEF patients.
Robotic Esophagectomy. A Systematic Review with Meta-Analysis of Clinical Outcomes
Authors: Manigrasso Michele , Vertaldi Sara , Marello Alessandra , Antoniou Stavros Athanasios , Francis Nader Kamal , De Palma Giovanni Domenico , Milone Marco
Published: July 2021

Background: Robot-Assisted Minimally Invasive Esophagectomy is demonstrated to be related with a facilitation in thoracoscopic procedure. To give an update on the state of art of robotic esophagectomy for cancr a systematic review with meta-analysis has been performed. Methods: a search of the studies comparing robotic and laparoscopic or open esophagectomy was performed trough the medical libraries, with the search string “robotic and (oesophagus OR esophagus OR esophagectomy OR oesophagectomy)”. Outcomes were: postoperative complications rate (anastomotic leakage, bleeding, wound infection, pneumonia, recurrent laryngeal nerves paralysis, chylotorax, mortality), intraoperative outcomes (mean blood loss, operative time and conversion), oncologic outcomes (harvested nodes, R0 resection, recurrence) and recovery outcomes (length of hospital stay). Results: Robotic approach is superior to open surgery in terms of blood loss p = 0.001, wound infection rate, p = 0.002, pneumonia rate, p = 0.030 and mean number of harvested nodes, p < 0.0001 and R0 resection rate, p = 0.043. Similarly, robotic approach is superior to conventional laparoscopy in terms of mean number of harvested nodes, p = 0.001 pneumonia rate, p = 0.003. Conclusions: robotic surgery could be considered superior to both open surgery and conventional laparoscopy. These encouraging results should promote the diffusion of the robotic surgery, with the creation of randomized trials to overcome selection bias.
Employee Organisational Commitment and the Mediating Role of Work Locus of Control and Employee Job Satisfaction: The Perspective of SME Workers
Authors: Mapuranga Miston , Maziriri Eugine Tafadzwa , Rukuni Tarisai Fritz , Lose Thobekani
Published: July 2021

In Southern Africa, employees play a critical role in the growth of small and medium enterprises (SMEs). As a result, SMEs’ effectiveness depends on the presence of virtuous workplace spirituality and procedural justice, which affects work locus of control, employee job satisfaction, and employee organisational commitment. The researchers looked at how work locus of control, employee job satisfaction, and employee organisational commitment were influenced by workplace spirituality and procedural justice among SME employees in Harare, Zimbabwe. The study data was quantitatively analysed. According to the results, workplace spirituality, procedural justice, and work locus of control all play a role in enhancing employee job satisfaction and commitment. The relationship between workplace spirituality, procedural justice, and employee organisational commitment was positively influenced by work locus of control and employee job satisfaction. The findings of this study are expected to provide valuable insights into the unique key determinants affecting work locus of control, employee job satisfaction, and employee commitment to the organisation.
A Comparative Analysis on Probability of Volatility Clusters on Cryptocurrencies, and FOREX Currencies
Authors: Chinthapalli Usha Rekha
Published: July 2021

In recent years, the attention of investors, practitioners and academics has grown in cryptocurrency. Initially, the cryptocurrency was designed as a viable digital currency implementation, and subsequently, numerous derivatives were produced in a range of sectors, including nonmonetary activities, financial transactions, and even capital management. The high volatility of exchange rates is one of the main features of cryptocurrencies. The article presents an interesting way to estimate the probability of cryptocurrency volatility clusters. In this regard, the paper explores exponential hybrid methodologies GARCH (or EGARCH) and through its portrayal as a financial asset, ANN models will provide analytical insight into bitcoin. Meanwhile, more scalable modelling is needed to fit financial variable characteristics such as ANN models because of the dynamic, nonlinear association structure between financial variables. For financial forecasting, BP is contained in the most popular methods of neural network training. The backpropagation method is employed to train the two models to determine which one performs the best in terms of predicting. This architecture consists of one hidden layer and one input layer with N neurons. Recent theoretical work on crypto-asset return behavior and risk management is supported by this research. In comparison with other traditional asset classes, these results give appropriate data on the behavior, allowing them to adopt the suitable investment decision. The study conclusions are based on a comparison between the dynamic features of cryptocurrencies and FOREX Currency’s traditional mass financial asset. Thus, the result illustrates how well the probability clusters show the impact on cryptocurrency and currencies. This research covers the sample period between August 2017 and August 2020, as cryptocurrency became popular around that period. The following methodology was implemented and simulated using Eviews and SPSS software. The performance evaluation of the cryptocurrencies is compared with FOREX currencies for better comparative study respectively.
To What Extent Are Cattle Ranching Landholders Willing to Restore Ecosystem Services? Constructing a Micro-Scale PES Scheme in Southern Costa Rica
Authors: Pérez-Rubio Iván , Flores Daniel , Vargas Christian , Jiménez Francisco , Etxano Iker
Published: July 2021

Deforestation and the unsustainable management of agricultural and livestock production systems in tropical mountain areas have caused fragmented and degraded landscapes. Payment for ecosystem services (PES) could be an effective policy instrument with which to reduce deforestation and restore disturbed ecosystems. The national-scale PES program in Costa Rica is recognized as being successful; however, its financial resources have been mostly dedicated to forest protection, and much less to reforestation projects. This paper aims to construct a micro-scale PES scheme by using primary data generated through spatial modeling and socio-economic and stated preference surveys (choice experiment) in southern Costa Rica. The results suggest that, on average, landholders would agree to implement restoration projects on their own private pasturelands if an appropriate holistic place-based approach was applied encompassing biophysical, social, economic, and institutional aspects. Willingness-to-accept values allow payments to be linked to cattle farmers’ estimates of specific ecosystem services (ES) and land opportunity costs. The economic valuation of three ESs (erosion control, water availability, and biodiversity) allows construction of a layered payment scheme, which could encourage the development of a potential partnership between national and local institutions and NGOs as alternative buyers of ESs, reduce transaction costs, and improve household well-being.
Forecasts of Opportunity for Northern California Soil Moisture
Authors: Penland Cécile , Fowler Megan D. , Jackson Darren L. , Cifelli Robert
Published: July 2021

Soil moisture anomalies underpin a number of critical hydrological phenomena with socioeconomic consequences, yet systematic studies of soil moisture predictability are limited. Here, we use a data-adaptive technique, Linear Inverse Modeling, which has proved useful as an indication of predictability in other fields, to investigate the predictability of soil moisture in northern California. This approach yields a model of soil moisture at 10 stations in the region, with results that indicate the possibility of skillful forecasts at each for lead times of 1–2 weeks. An important advantage of this model is the a priori identification of forecasts of opportunity—conditions under which the model’s forecasts may be expected to have particularly high skill. Given that forecast errors (and inversely, their skill) can be estimated in advance, these findings have the potential to greatly increase the utility of soil moisture forecasts for practical applications including drought and flood forecasting.
F4-Neuroprostanes: A Role in Sperm Capacitation
Authors: Signorini Cinzia , Moretti Elena , Noto Daria , Mattioli Simona , Castellini Cesare , Pascarelli Nicola Antonio , Durand Thierry , Oger Camille , Galano Jean-Marie , De Felice Claudio , Lee Jetty Chung-Yung , Collodel Giulia
Published: July 2021

F4-neuroprostanes (F4-NeuroPs), derived from the oxidative metabolization of docosahexaenoic acid (DHA), are considered biomarkers of oxidative stress in neurodegenerative diseases. Neurons and spermatozoa display a high DHA content. NeuroPs might possess biological activities. The aim of this in vitro study was to investigate the biological effects of chemically synthetized 4-F4t-NeuroP and 10-F4t-NeuroP in human sperm. Total progressive sperm motility (p < 0.05) and linearity (p = 0.016), evaluated by a computer-assisted sperm analyzer, were significantly increased in samples incubated with 7 ng F4-NeuroPs compared to non-supplemented controls. Sperm capacitation was tested in rabbit and swim-up-selected human sperm by chlortetracycline fluorescence assay. A higher percentage of capacitated sperm (p < 0.01) was observed in samples incubated in F4-NeuroPs than in the controls. However, the percentage of capacitated sperm was not different in F4-NeuroPs and calcium ionophore treatments at 2 h incubation. The phosphorylated form of AMPKα was detected by immunofluorescence analysis; after 2 h F4-NeuroP incubation, a dotted signal appeared in the entire sperm tail, and in controls, sperm were labeled in the mid-piece. A defined level of seminal F4-NeuroPs (7 ng) showed a biological activity in sperm function; its addition in sperm suspensions stimulated capacitation, increasing the number of sperm able to fertilize.
Multigene Phylogenetics and Morphology Reveal Five Novel Lasiodiplodia Species Associated with Blueberries
Authors: Wang Yu , Zhang Ying , Bhoyroo Vishwakalyan , Rampadarath Sillma , Jeewon Rajesh
Published: July 2021

Botryosphaeriaceous fungi cause stem blight, canker and dieback in woody plants. During a survey on the fungal pathogens associated with blueberries in China, 135 blighted, cankered or dead blueberry branches were collected from Fujian and Shandong Provinces. Based on the morphological characterization and phylogenetic analyses of a concatenated ITS rDNA, tef1-α, TUB, and RPB2 loci, five new species of Lasiodiplodia, viz., L. clavispora, L. fujianensis, L. henanica, L. nanpingensis and L. paraphysoides were recognized. Detailed descriptions and illustrations, as well as multigene phylogenies, are provided in this paper. The diversity of plant pathogens on agriculturally and economically important plants is higher than anticipated.
Inadvertent Detachment of Stent Retrievers during Mechanical Thrombectomy—A Clinical and Biomechanical Perspective
Authors: Piasecki Piotr , Wierzbicki Marek , Tulik Piotr , Potocka Katarzyna , Stępień Adam , Staszewski Jacek , Dębiec Aleksander , Narloch Jerzy
Published: July 2021

Background: The inadvertent detachment of stent retrievers during mechanical thrombectomy is an extremely rare but feared complication associated with poor clinical outcomes. We discuss management considerations after an unexpected disconnection of the pRESET stent retriever during mechanical thrombectomy, based on clinical experience and mechanical and phantom studies. Methods: We present a clinical course of rare accidents of stent-retriever separation inside an intracranial vessel that occurred in patients in a comprehensive stroke centre between 2018 and 2020. We designed a phantom study to assess the Tigertriever’s ability to remove a detached stent retriever from intercranial vessels. In the mechanical study, several types of stent retrievers were evaluated in order to find the weakest point at which detachment occurred. Results: Two patients (~0.7%) with inadvertent stent-retriever detachment were found in our database. Failed attempts of endovascular removal with no recanalization at the end of procedure were reported in both cases. mRS after 3 months was three and four respectively. In the mechanical study, the Tigertriever was the most resistant to detachment and was followed by Embotrap > pRESET > 3D Separator. In the phantom study, the pRESET device detached in a configuration resembling the M1 segment was successfully removed with the Tigertriever. Conclusions: Conservative management of the inadvertent detachment of stent retrievers during mechanical thrombectomy in large vessel occlusion may be acceptable in order to avoid further periprocedural complications after unsuccessful device removal attempts. Based on the phantom and mechanical studies, the Tigertriever may be a useful tool for the removal of detached pRESET devices.
Analytical Performance of COVID-19 Detection Methods (RT-PCR): Scientific and Societal Concerns
Authors: Verna Roberto , Alallon Walter , Murakami Masami , Hayward Catherine P. M. , Harrath Abdel Halim , Alwasel Saleh H. , Sumita Nairo M. , Alatas Ozkan , Fedeli Valeria , Sharma Praveen , Fuso Andrea , Capuano Daniela Maria , Capalbo Maria , Angeloni Antonio , Bizzarri Mariano
Published: July 2021

Background. Health and social management of the SARS-CoV-2 epidemic, responsible for the COVID-19 disease, requires both screening tools and diagnostic procedures. Reliable screening tests aim at identifying (truely) infectious individuals that can spread the viral infection and therefore are essential for tracing and harnessing the epidemic diffusion. Instead, diagnostic tests should supplement clinical and radiological findings, thus helping in establishing the diagnosis. Several analytical assays, mostly using RT-PCR-based technologies, have become commercially available for healthcare workers and clinical laboratories. However, such tests showed some critical limitations, given that a relevant number of both false-positive and false-negative cases have been so far reported. Moreover, those analytical techniques demonstrated to be significantly influenced by pre-analytical biases, while the sensitivity showed a dramatic time dependency. Aim. Herein, we critically investigate limits and perspectives of currently available RT-PCR techniques, especially when referring to the required performances in providing reliable epidemiological and clinical information. Key Concepts. Current data cast doubt on the use of RT-PCR swabs as a screening procedure for tracing the evolution of the current SARS-COV-2 pandemic. Indeed, the huge number of both false-positive and false-negative results deprives the trustworthiness of decision making based on those data. Therefore, we should refine current available analytical tests to quickly identify individuals able to really transmit the virus, with the aim to control and prevent large outbreaks.
Applications of PERALS-Alpha Spectrometry for the Investigation of Radionuclides in Water Samples
Authors: Zehringer Markus , Kammerer Franziska , Pregler Anja
Published: July 2021

In this paper, experiences of the last 20 years with the PERALS-technique are described. PERALS stands for photo electron-rejecting alpha liquid scintillation. This liquid scintillation technique was developed by Jack McDowell in the 1970s and is a powerful technique for the analyses of many natural alpha nuclides and also the beta nuclide 90Sr. The principle is based on a selective extraction of the radionuclide from the water phase by means of a complexing or ion pair reagent. The extractant contains also a cocktail suitable for scintillation counting. Therefore, the extract can be analyzed directly after the extraction step. After removing quenchers, such as oxygen, and the proper setting of a pulse shape discriminator, alpha pulses can be counted with a photomultiplier. This paper describes the development of robust analysis schemes for the determination of traces of polonium, thorium, uranium and other actinides in water samples (groundwater, rain water, river water, drinking water, mineral water, sea water). For radon and radium, the enrichment in the extract is poor. Therefore, PERALS methods are not suitable for trace analyses of these analytes. In addition, the extraction of the beta-emitter 90Sr with a PERALS cocktail is discussed, even though its beta spectrum is not analyzed with a PERALS counter. Results from the survey of drinking water and mineral water in Switzerland are presented for every radio element.
Hybrid Optimization Based Mathematical Procedure for Dimensional Synthesis of Slider-Crank Linkage
Authors: Hernández Alfonso , Muñoyerro Aitor , Urízar Mónica , Amezua Enrique
Published: July 2021

In this paper, an optimization procedure for path generation synthesis of the slider-crank mechanism will be presented. The proposed approach is based on a hybrid strategy, mixing local and global optimization techniques. Regarding the local optimization scheme, based on the null gradient condition, a novel methodology to solve the resulting non-linear equations is developed. The solving procedure consists of decoupling two subsystems of equations which can be solved separately and following an iterative process. In relation to the global technique, a multi-start method based on a genetic algorithm is implemented. The fitness function incorporated in the genetic algorithm will take as arguments the set of dimensional parameters of the slider-crank mechanism. Several illustrative examples will prove the validity of the proposed optimization methodology, in some cases achieving an even better result compared to mechanisms with a higher number of dimensional parameters, such as the four-bar mechanism or the Watt’s mechanism.
Polyelectrolyte Functionalisation of Track Etched Membranes: Towards Charge-Tuneable Adsorber Materials
Authors: Wiedenhöft Lisa , Elleithy Mohamed M. A. , Ulbricht Mathias , Schacher Felix H.
Published: July 2021

Porous adsorber membranes are promising materials for the removal of charged pollutants, such as heavy metal ions or organic dyes as model substances for pharmaceuticals from water. Here, we present the surface grafting of polyethylene terephthalate (PET) track-etched membranes having well defined cylindrical pores of 0.2 or 1 µm diameter with two polyelectrolytes, poly(2-acrylamido glycolic acid) (PAGA) and poly(N-acetyl dehydroalanine) (PNADha). The polyelectrolyte functionalised membranes were characterised by changes in wettability and hydraulic permeability in response to the external stimuli pH and the presence of Cu2+ ions. The response of the membranes proved to be consistent with functionalisation inside the pores, and the change of grafted polyelectrolyte macro-conformation was due to the reversible protonation or binding of Cu2+ ions. Moreover, the adsorption of the model dye methylene blue was studied and quantified. PAGA-grafted membranes showed an adsorption behavior following the Langmuir model for methylene blue.
Selective Laser Melting of 316L Austenitic Stainless Steel: Detailed Process Understanding Using Multiphysics Simulation and Experimentation
Authors: Ansari Peyman , Rehman Asif Ur , Pitir Fatih , Veziroglu Salih , Mishra Yogendra Kumar , Aktas Oral Cenk , Salamci Metin U.
Published: July 2021

The parameter sets used during the selective laser melting (SLM) process directly affect the final product through the resulting melt-pool temperature. Achieving the optimum set of parameters is usually done experimentally, which is a costly and time-consuming process. Additionally, controlling the deviation of the melt-pool temperature from the specified value during the process ensures that the final product has a homogeneous microstructure. This study proposes a multiphysics numerical model that explores the factors affecting the production of parts in the SLM process and the mathematical relationships between them, using stainless steel 316L powder. The effect of laser power and laser spot diameter on the temperature of the melt-pool at different scanning velocities were studied. Thus, mathematical expressions were obtained to relate process parameters to melt-pool temperature. The resulting mathematical relationships are the basic elements to design a controller to instantly control the melt-pool temperature during the process. In the study, test samples were produced using simulated parameters to validate the simulation approach. Samples produced using simulated parameter sets resulting in temperatures of 2000 K and above had acceptable microstructures. Evaporation defects caused by extreme temperatures, unmelted powder defects due to insufficient temperature, and homogenous microstructures for suitable parameter sets predicted by the simulations were obtained in the experimental results, and the model was validated.
Analysis of Microstructure and Mechanical Properties of Bismuth-Doped SAC305 Lead-Free Solder Alloy at High Temperature
Authors: Ali Umair , Khan Hamza , Aamir Muhammad , Giasin Khaled , Habib Numan , Owais Awan Muhammad
Published: July 2021

SAC305 lead-free solder alloy is widely used in the electronic industry. However, the problems associated with the growth formation of intermetallic compounds need further research, especially at high temperatures. This study investigates the doping of Bismuth into SAC305 in the various compositions of 1, 2, and 3 wt.%. The microstructure in terms of intermetallic compound particles and mechanical properties was examined after thermal aging at temperatures of 100 °C and 200 °C for 60 h. The microstructure examination was observed using scanning electron microscopy, and the chemical composition of each alloy was confirmed with an energy dispersive X-ray. Tensile tests were performed to find the mechanical properties such as yield strength and ultimate tensile strength. The intermetallic compound’s phase analysis was identified using X-ray diffraction, and differential scanning calorimetry was done to study the temperature curves for melting points. Results showed that the addition of Bismuth refined the microstructure by suppressing the growth of intermetallic compounds, which subsequently improved the mechanical properties. The thermal aging made the microstructure coarsen and degraded the mechanical properties. However, the most improved performance was observed with a Bismuth addition of 3 wt.% into SAC305. Furthermore, a decrease in the melting temperature was observed, especially at Bismuth compositions of 3 wt.%.
Unveiling the Link between the Third Law of Comminution and the Grinding Kinetics Behaviour of Several Ores
Authors: Ciribeni Victor , Menéndez-Aguado Juan M. , Bertero Regina , Tello Andrea , Avellá Enzo , Paez Matías , Coello-Velázquez Alfredo L.
Published: July 2021

As a continuation of a previous research work carried out to estimate the Bond work index (wi) by using a simulator based on the cumulative kinetic model (CKM), a deeper analysis was carried out to determine the link between the kinetic and energy parameters in the case of metalliferous and non-metallic ore samples. The results evidenced a relationship between the CKM kinetic parameter k and the grindability index gbp; and also with the wi, obtained following the standard procedure. An excellent correlation was obtained in both cases, posing the definition of alternative work index estimation tests with the advantages of more straightforward and quicker laboratory procedures.
Impact on Bile Acid Concentrations by Alveolar Echinococcosis and Treatment with Albendazole in Mice
Authors: Gómez Cristina , Jebbawi Fadi , Weingartner Michael , Wang Junhua , Stücheli Simon , Stieger Bruno , Gottstein Bruno , Beldi Guido , Lundström-Stadelmann Britta , Odermatt Alex
Published: July 2021

Alveolar echinococcosis (AE) caused by Echinococcus multilocularis is a chronic, progressive liver disease widely distributed in the Northern Hemisphere. The main treatment options include surgical interventions and chemotherapy with benzimidazole albendazole (ABZ). To improve the current diagnosis and therapy of AE, further investigations into parasite–host interactions are needed. This study used liquid chromatography–tandem mass spectrometry (LC-MS/MS) to assess serum and liver tissue bile acid profiles in the i.p. chronic E. multilocularis-infected mouse model and evaluated the effects of the anthelmintic drug ABZ. Additionally, hepatic mRNA and protein expression of enzymes and transporters regulating bile acid concentrations were analyzed. AE significantly decreased unconjugated bile acids in serum and liver tissue. Taurine-conjugated bile salts were unchanged or increased in the serum and unchanged or decreased in the liver. Ratios of unconjugated to taurine-conjugated metabolites are proposed as useful serum markers of AE. The expression of the bile acid synthesis enzymes cytochrome P450 (CYP) 7A1 and aldo-keto reductase (AKR) 1D1 tended to decrease or were decreased in mice with AE, along with decreased expression of the bile acid transporters Na+/taurocholate cotransporting polypeptide (NTCP) and bile salt efflux pump (BSEP). Importantly, treatment with ABZ partially or completely reversed the effects induced by E. multilocularis infection. ABZ itself had no effect on the bile acid profiles and the expression of relevant enzymes and transporters. Further research is needed to uncover the exact mechanism of the AE-induced changes in bile acid homeostasis and to test whether serum bile acids and ratios thereof can serve as biomarkers of AE and for monitoring therapeutic efficacy.
The Boundary Proportion Differential Control Method of Micro-Deformable Manipulator with Compensator Based on Partial Differential Equation Dynamic Model
Authors: Pei Xiangli , Tian Ying , Zhang Minglu , Shi Ruizhuo
Published: July 2021

It is challenging to accurately judge the actual end position of the manipulator—regarded as a rigid body—due to the influence of micro-deformation. Its precise and efficient control is a crucial problem. To solve the problem, the Hamilton principle was used to establish the partial differential equation (PDE) dynamic model of the manipulator system based on the infinite dimension of the working environment interference and the manipulator space. Hence, it resolves the common overflow instability problem in the micro-deformable manipulator system modeling. Furthermore, an infinite-dimensional radial basis function neural network compensator suitable for the dynamic model was proposed to compensate for boundary and uncertain external interference. Based on this compensation method, a distributed boundary proportional differential control method was designed to improve control accuracy and speed. The effectiveness of the proposed model and method was verified by theoretical analysis, numerical simulation, and experimental verification. The results show that the proposed method can effectively improve the response speed while ensuring accuracy.
Pre and Probiotics Involved in the Modulation of Oral Bacterial Species: New Therapeutic Leads in Mental Disorders?
Authors: Maitre Yoann , Mahalli Rachid , Micheneau Pierre , Delpierre Alexis , Guerin Marie , Amador Gilles , Denis Frédéric
Published: July 2021

This systematic review aims to identify probiotics and prebiotics for modulating oral bacterial species associated with mental disorders. Using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis guideline, we search the electronic MEDLINE database published till January 2021 to identify the studies on probiotics and/or prebiotics for preventing and treating major oral dysbiosis that provokes mental disorders. The outcome of the search produces 374 records. After excluding non-relevant studies, 38 papers were included in the present review. While many studies suggest the potential effects of the oral microbiota on the biochemical signalling events between the oral microbiota and central nervous system, our review highlights the limited development concerning the use of prebiotics and/or probiotics in modulating oral dysbiosis potentially involved in the development of mental disorders. However, the collected studies confirm prebiotics and/or probiotics interest for a global or targeted modulation of the oral microbiome in preventing or treating mental disorders. These outcomes also offer exciting prospects for improving the oral health of people with mental disorders in the future.
A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers
Authors: Kumar Alip , Kheravii Sarbast K. , Ionescu Catherine , Blanchard Alexandra , Barekatain Reza , Bajagai Yadav S. , Wu Shu-Biao
Published: July 2021

This study was conducted to examine the effects of a plant extract mixture, a microencapsulated product composed of eugenol and garlic tincture (PE), on intestinal health in broilers under necrotic enteritis (NE) challenge. A total of 960 d-old mixed-sex Cobb 500 chicks were randomly distributed to 48-floor pens housing 20 birds per pen. Six treatments were applied: UC, unchallenged control; CC, challenged control; PE, challenged group plus PE; AM, challenged group plus antimicrobial (AM); FAP, challenged group plus a full dose of AM with PE; HAP, challenged group plus a half dose of AM with PE in starter, grower and finisher phases. Birds in the challenged groups were inoculated with Eimeria spp. on d 9 and Clostridiumperfringens on d 14. On d 16, the CC group had increased serum fluorescein isothiocyanate dextran (FITC-d), reduced villus surface area, goblet cell number, upregulated CLDN1, JAM2 genes and reduced microbial diversity compared to the UC group (p < 0.05). Birds fed PE had reduced FITC-d, increased goblet cell number and Bifidobacterium compared to the CC group (p < 0.05). Birds fed PE had reduced CLDN5 expression in male birds, and Bacteroides spp. in female birds than CC group (p < 0.05). These findings suggest that PE supplementation mitigates the effect of NE by improving the intestinal health of birds.
Mineralogy and Geochemistry of Ocelli in the Damtjernite Dykes and Sills, Chadobets Uplift, Siberian Craton: Evidence of the Fluid–Lamprophyric Magma Interaction
Authors: Nosova Anna A. , Sazonova Ludmila V. , Kargin Alexey V. , Dubinina Elena O. , Minervina Elena A.
Published: July 2021

The study reports petrography, mineralogy and carbonate geochemistry and stable isotopy of various types of ocelli (silicate-carbonate globules) observed in the lamprophyres from the Chadobets Uplift, southwestern Siberian craton. The Chadobets lamprophyres are related to the REE-bearing Chuktukon carbonatites. On the basis of their morphology, mineralogy and relation with the surrounding groundmass, we distinguish three types of ocelli: carbonate-silicate, containing carbonate, scapolite, sodalite, potassium feldspar, albite, apatite and minor quartz ocelli (K-Na-CSO); carbonate–silicate ocelli, containing natrolite and sodalite (Na-CSO); and silicate-carbonate, containing potassium feldspar and phlogopite (K-SCO). The K-Na-CSO present in the most evolved damtjernite with irregular and polygonal patches was distributed within the groundmass; the patches consist of minerals identical to minerals in ocelli. Carbonate in the K-Na-CSO are calcite, Fe-dolomite and ankerite with high Sr concentration and igneous-type REE patterns. The Na-CSO present in Na-rich damtjernite with geochemical signature indicates the loss of the carbonate component. Carbonate phases are calcite and Fe-dolomite, and they depleted in LREE. The K-SCO was present in the K-rich least-evolved damtjernite. Calcite in the K-SCO has the highest Ba and the lowest Sr concentration and U-shaped REE pattern. The textural, mineralogical and geochemical features of the ocelli and their host rock can be interpreted as follows: (i) the K-Na-CSO are droplets of an alkali–carbonate melt that separated from residual alkali and carbonate-rich melt in highly evolved damtjernite; (ii) the Na-CSO are droplets of late magmatic fluid that once exsolved from a melt and then began to dissolve; (iii) the K-SCO are bubbles of K-P-CO2 fluid liberated from an almost-crystallised magma during the magmatic–hydrothermal stage. The geochemical signature of the K-SCO carbonate shows that the late fluid could leach REE from the host lamprophyre and provide for REE mobility.
Preseismic Groundwater Ion Content Variations: Observational Data in Flowing Wells of the Kamchatka Peninsula and Conceptual Model
Authors: Kopylova Galina , Boldina Svetlana
Published: July 2021

Repeated manifestations of hydrogeochemical anomalies in groundwater ion content variations before local strong earthquakes were recorded in three flowing wells of the Petropavlovsk-Kamchatsky test site. A model of changes in chemical composition of groundwater is considered using observational data and modeling of two waters mixing with contrasting composition in a zone of increased permeability in aquifer. Hydrodynamic parameters of the model—relaxation time of water pressure impulses and the time of mixed water movement in aquifer and wellbore—can vary from days to hundreds of days in individual wells. These parameters determine the duration and morphological features of anomaly in mixed water from a well. Using observational data from self-flowing well M-1, an assessment was made of chemical composition and mineralization of two mixing waters and their ratio in mixed water under background conditions and during the preparation of earthquake on 2 March 1992, Mw = 6.9.
Plasma and Polymers: Recent Progress and Trends
Authors: Levchenko Igor , Xu Shuyan , Baranov Oleg , Bazaka Olha , Ivanova Elena , Bazaka Kateryna
Published: July 2021

Plasma-enhanced synthesis and modification of polymers is a field that continues to expand and become increasingly more sophisticated. The highly reactive processing environments afforded by the inherently dynamic nature of plasma media are often superior to ambient or thermal environments, offering substantial advantages over other processing methods. The fluxes of energy and matter toward the surface enable rapid and efficient processing, whereas the charged nature of plasma-generated particles provides a means for their control. The range of materials that can be treated by plasmas is incredibly broad, spanning pure polymers, polymer-metal, polymer-wood, polymer-nanocarbon composites, and others. In this review, we briefly outline some of the recent examples of the state-of-the-art in the plasma-based polymer treatment and functionalization techniques.
Metal Nanoparticles as Sustainable Tools for C–N Bond Formation via C–H Activation
Authors: Valentini Federica , Piermatti Oriana , Vaccaro Luigi
Published: July 2021

The design of highly active metal nanoparticles to be employed as efficient heterogeneous catalysts is a key tool for the construction of complex organic molecules and the minimization of their environmental costs. The formation of novel C–N bonds via C–H activation is an effective atom-economical strategy to access high value materials in pharmaceuticals, polymers, and natural product production. In this contribution, the literature of the last ten years on the use of metal nanoparticles in the processes involving direct C–N bond formation will be discussed. Where possible, a discussion on the role and influence of the support used for the immobilization and/or the metal chosen is reported. Particular attention was given to the description of the experiments performed to elucidate the active mechanism.