bluuebunny/crossref_metadata_2025 huggingface dataset:
Applications of Enzyme Technology to Enhance Transition to Plant Proteins: A Review
Authors: Gouseti Ourania , Larsen Mads Emil , Amin Ashwitha , Bakalis Serafim , Petersen Iben Lykke , Lametsch Rene , Jensen Poul Erik
Published: June 2023

As the plant-based food market grows, demand for plant protein is also increasing. Proteins are a major component in foods and are key to developing desired structures and textures. Seed storage proteins are the main plant proteins in the human diet. They are abundant in, for example, legumes or defatted oilseeds, which makes them an excellent candidate to use in the development of novel plant-based foods. However, they often have low and inflexible functionalities, as in nature they are designed to remain densely packed and inert within cell walls until they are needed during germination. Enzymes are often used by the food industry, for example, in the production of cheese or beer, to modify ingredient properties. Although they currently have limited applications in plant proteins, interest in the area is exponentially increasing. The present review first considers the current state and potential of enzyme utilization related to plant proteins, including uses in protein extraction and post-extraction modifications. Then, relevant opportunities and challenges are critically discussed. The main challenges relate to the knowledge gap, the high cost of enzymes, and the complexity of plant proteins as substrates. The overall aim of this review is to increase awareness, highlight challenges, and explore ways to address them.
Non-Destructive Classification of Organic and Conventional Hens’ Eggs Using Near-Infrared Hyperspectral Imaging
Authors: Sahachairungrueng Woranitta , Thompson Anthony Keith , Terdwongworakul Anupun , Teerachaichayut Sontisuk
Published: June 2023

Eggs that are produced using organic methods retail at higher prices than those produced using conventional methods, but they cannot be differentiated reliably using visual methods. Eggs can therefore be fraudulently mislabeled in order to increase their wholesale and retail prices. The objective of this research was therefore to test near-infrared hyperspectral imaging (NIR-HSI) to identify whether an egg has been produced using organic or conventional methods. A total of 210 organic and 210 conventional fresh eggs were individually scanned using NIR-HSI to obtain absorbance spectra for discrimination analysis. The physical properties of each egg were also measured non-destructively in order to analyze the performance of discrimination compared with those of the NIR-HSI spectral data. Principal component analysis (PCA) showed variation for PC1 and PC2 of 57% and 23% and 94% and 4% based on physical properties and the spectral data, respectively. The best results of the classification using NIR-HSI spectral data obtained an accuracy of 96.03% and an error rate of 3.97% via partial least squares–discriminant analysis (PLS-DA), indicating the possibility that NIR-HSI could be successfully used to rapidly, reliably, and non-destructively differentiate between eggs that had been produced using organic methods from eggs that had been produced using conventional methods.
Development of Films from Spent Coffee Grounds’ Polysaccharides Crosslinked with Calcium Ions and 1,4-Phenylenediboronic Acid: A Comparative Analysis of Film Properties and Biodegradability
Authors: Batista Michelle J. P. A. , Marques M. Betânia F. , Franca Adriana S. , Oliveira Leandro S.
Published: June 2023

Most polymeric materials are synthetic and derived from petroleum, hence they accumulate in landfills or the ocean, and recent studies have focused on alternatives to replace them with biodegradable materials from renewable sources. Biodegradable wastes from food and agroindustry, such as spent coffee grounds (SCGs), are annually discarded on a large scale and are rich in organic compounds, such as polysaccharides, that could be used as precursors to produce films. Around 6.5 million tons of SCGs are discarded every year, generating an environmental problem around the world. Therefore, it was the aim of this work to develop films from the SCGs polysaccharide fraction, which is comprised of cellulose, galactomannans and arabinogalactans. Two types of crosslinking were performed: the first forming coordination bonds of calcium ions with polysaccharides; and the second through covalent bonds with 1,4-phenylenediboronic acid (PDBA). The films with Ca2+ ions exhibited a greater barrier to water vapor with a reduction of 44% of water permeability vapor and 26% greater tensile strength than the control film (without crosslinkers). Films crosslinked with PDBA presented 55–81% higher moisture contents, 85–125% greater permeability to water vapor and 67–150% larger elongations at break than the films with Ca2+ ions. Film biodegradability was demonstrated to be affected by the crosslinking density, with the higher the crosslinking density, the longer the time for the film to fully biodegrade. The results are promising and suggest that future research should focus on enhancing the properties of these films to expand the range of possible applications.
The Influence of Storage and Cooking on the Vitamin D Content of 25-Hydroxyvitamin D3-Enriched Eggs
Authors: Clark Adam , Kuznesof Sharron , Waller Anthony , Davies Sarah , Wilson Simon , Ritchie Avril , Duesterloh Andre , Harbord Lance , Hill Thomas Robert
Published: June 2023

Food fortification is an effective approach to improve vitamin D (VD) concentrations in foods. Eggs are a useful food vehicle for enrichment with VD via its hydroxylated metabolite, 25-hydroxyvitamin D (25-D3), in hen feed. This study determined the impact of time of lay, storage conditions (ambient and refrigeration) and common cooking methods (boiling, frying, scrambling, poaching and microwaving) on the vitamin D metabolite concentration of eggs enriched with 25-D3. Processed samples were freeze-dried and analysed for D3 and 25-D3 using an HPLC-MS(/MS) method. The results indicated that storage and cooking practices influence VD metabolites, with 25-D3 showing true retention of 72–111% and concentrations of 0.67–0.96 µg/100 g of whole egg. Vitamin D3 showed true retention of 50–152% and concentrations of 0.11–0.61 µg/100 g of whole egg. Depending on the storage and method of cooking applied, the calculated total VD activity of enriched eggs ranged from 3.45 to 5.43 µg/100 g of whole egg and was 22–132% higher in comparison to standardised VD content for non-enriched British eggs. The study suggests that 25-D3 is a stable metabolite in eggs following storage and cooking, and that 25-D3-enriched eggs may serve as a potent dietary source of VD.
A Comprehensive Review of Pea (Pisum sativum L.): Chemical Composition, Processing, Health Benefits, and Food Applications
Authors: Wu Ding-Tao , Li Wen-Xing , Wan Jia-Jia , Hu Yi-Chen , Gan Ren-You , Zou Liang
Published: June 2023

Pisum sativum L., commonly referred to as dry, green, or field pea, is one of the most common legumes that is popular and economically important. Due to its richness in a variety of nutritional and bioactive ingredients, the consumption of pea has been suggested to be associated with a wide range of health benefits, and there has been increasing focus on its potential as a functional food. However, there have been limited literature reviews concerning the bioactive compounds, health-promoting effects, and potential applications of pea up to now. This review, therefore, summarizes the literature from the last ten years regarding the chemical composition, physicochemical properties, processing, health benefits, and potential applications of pea. Whole peas are rich in macronutrients, including proteins, starches, dietary fiber, and non-starch polysaccharides. In addition, polyphenols, especially flavonoids and phenolic acids, are important bioactive ingredients that are mainly distributed in the pea coats. Anti-nutritional factors, such as phytic acid, lectin, and trypsin inhibitors, may hinder nutrient absorption. Whole pea seeds can be processed by different techniques such as drying, milling, soaking, and cooking to improve their functional properties. In addition, physicochemical and functional properties of pea starches and pea proteins can be improved by chemical, physical, enzymatic, and combined modification methods. Owing to the multiple bioactive ingredients in peas, the pea and its products exhibit various health benefits, such as antioxidant, anti-inflammatory, antimicrobial, anti-renal fibrosis, and regulation of metabolic syndrome effects. Peas have been processed into various products such as pea beverages, germinated pea products, pea flour-incorporated products, pea-based meat alternatives, and encapsulation and packing materials. Furthermore, recommendations are also provided on how to better utilize peas to promote their development as a sustainable and functional grain. Pea and its components can be further developed into more valuable and nutritious products.
Thermal Behavior of Pea and Egg White Protein Mixtures
Authors: Kuang Jian , Hamon Pascaline , Lechevalier Valérie , Saurel Rémi
Published: June 2023

The partial substitution of animal protein by plant protein is a new opportunity to produce sustainable food. Hence, to control the heat treatment of a composite protein ingredient, this work investigated the thermal behavior of mixtures of raw egg white (EW) and a laboratory-prepared pea protein isolate (PPI). Ten-percentage-by-weight protein suspensions prepared with different PPI/EW weight ratios (100/0, 75/25, 50/50, 25/75, 0/100) at pH 7.5 and 9.0 were analyzed by differential scanning calorimetry (DSC) and dynamic rheology in temperature sweep mode (T < 100 °C). The DSC data revealed changes in the thermal denaturation temperatures (Td) of ovotransferrin, lysozyme, and pea legumin, supposing interactions between proteins. Denaturation enthalpy (∆H) showed a high pH dependence related to pea protein unfolding in alkaline conditions and solubility loss of some proteins in admixture. Upon temperature sweeps (25–95 °C), the elastic modulus (G′) of the mixtures increased significantly with the EW content, indicating that the gel formation was governed by the EW protein. Two thermal sol–gel transitions were found in EW-containing systems. In particular, the first sol–gel transition shifted by approximately +2–3 °C at pH 9.0, probably by a steric hindering effect due to the presence of denatured and non-associated pea globulins at this pH.
Parameter Estimation in Rough Bessel Model
Authors: Mishura Yuliya , Yurchenko-Tytarenko Anton
Published: June 2023

In this paper, we construct consistent statistical estimators of the Hurst index, volatility coefficient, and drift parameter for Bessel processes driven by fractional Brownian motion with H<1/2. As an auxiliary result, we also prove the continuity of the fractional Bessel process. The results are illustrated with simulations.
Probing Families of Optical Soliton Solutions in Fractional Perturbed Radhakrishnan–Kundu–Lakshmanan Model with Improved Versions of Extended Direct Algebraic Method
Authors: Yasmin Humaira , Aljahdaly Noufe H. , Saeed Abdulkafi Mohammed , Shah Rasool
Published: June 2023

In this investigation, we utilize advanced versions of the Extended Direct Algebraic Method (EDAM), namely the modified EDAM (mEDAM) and r+ mEDAM, to explore families of optical soliton solutions in the Fractional Perturbed Radhakrishnan–Kundu–Lakshmanan Model (FPRKLM). Our study stands out due to its in-depth investigation and the identification of multiple localized and stable soliton families, illuminating their complex behavior. We offer visual validation via carefully designed 3D graphics that capture the complex behaviors of these solitons. The implications of our research extend to fiber optics, communication systems, and nonlinear optics, with the potential for driving developments in optical devices and information processing technologies. This study conveys an important contribution to the field of nonlinear optics, paving the way for future advancements and a greater comprehension of optical solitons and their applications.
On Further Inequalities for Convex Functions via Generalized Weighted-Type Fractional Operators
Authors: Yıldız Çetin , Rahman Gauhar , Cotîrlă Luminiţa-Ioana
Published: June 2023

Several inequalities for convex functions are derived in this paper using the monotonicity properties of functions and a generalized weighted-type fractional integral operator, which allows the integration of a function κ with respect to another function in fractional order. Additionally, it is clear that the results were generalizations of the previously presented findings. In addition, different types of inequalities are obtained using the basic features of mathematical analysis. Finally, we believe that the methodology used in this work will inspire additional research in this field.
Polysaccharide-Based Hydrogel from Seeds of Artemisia vulgaris: Extraction Optimization by Box–Behnken Design, pH-Responsiveness, and Sustained Drug Release
Authors: Hussain Muhammad Ajaz , Ali Arshad , Alsahli Tariq G. , Khan Nadia , Sharif Ahsan , Haseeb Muhammad Tahir , Alsaidan Omar Awad , Tayyab Muhammad , Bukhari Syed Nasir Abbas
Published: June 2023

The current research work focuses on the extraction and optimization of the hydrogel (AVM) from the seeds of Artemisia vulgaris using Box–Behnken design-response surface methodology (BBD-RSM). The AVM was obtained through a hot water extraction process. The influence of different factors, including pH (U = 4 to 10), temperature (V = 25 to 110 °C), seed/water ratio, i.e., S/W ratio (W = 1/10 to 1/70 w/v), and seed/water contact time, i.e., S/W time (X = 1 to 12 h) on the yield of AVM was evaluated. The p-value for the analysis of variance (ANOVA) was found to be <0.001, indicating that the yield of AVM mainly depended on the abovementioned factors. The highest yield of AVM, i.e., 15.86%, was found at a pH of 7.12, temperature of 80.04 °C, S/W ratio of 1/33.24 w/v, and S/W time of 8.73 h according to Design-Expert Software. The study of the pH-responsive behavior of AVM in tablet form (formulation AVT3) revealed that AVM is a pH-responsive material with significantly high swelling at pH 7.4. However, less swelling was witnessed at pH 1.2. Moreover, AVM was found to be a sustained release material for esomeprazole at pH 7.4 for 12 h. The drug release from AVT3 was according to the super case-II transport mechanism and zero-order kinetics.
Hyaluronic Acid–Alginate Homogeneous Structures with Polylactide Coating Applied in Controlled Antibiotic Release
Authors: Trusek Anna , Grabowski Maciej , Ajayi Omoyemi , Kijak Edward
Published: June 2023

The use of a controlled-release drug carrier is an innovative solution for the treatment of local infections, in particular in dentistry, skin diseases, and in open wounds. The biocompatibility, biodegradability, the possibility of a large amount of drug adsorbed (especially those with hydrophilic properties), and the ability to create structures of any shape and size are the reasons for hydrogels to be frequently studied. The main disadvantage of hydrogel carriers is the rapid rate of drug release; hence, in this study, an attempt was made to additionally chemically cross-link 1-ethyl-3-(3-dimethyl aminopropyl)-1-carbodiimide hydrochloride (EDC) with the hyaluronic acid–alginate (HA–SAL) structure. The answer to significantly reduce the mass flux typical for hydrogel structure was to surround it with a polymer layer using a dry cover. By coating the carriers with polylactide, the release time was increased by around forty times. As the carriers were designed to reduce local bacterial infections, among others in dentistry, the released antibiotics were amoxycillin, metronidazole, and doxycycline.
Integrating 4Ms Assessment through Medicare Annual Wellness Visits: Comparison of Quality Improvement Strategies in Primary Care Clinics
Authors: Tewary Sweta , Cook Nicole , Simon Desiree , Philippe Elizabeth , Shnayder Oksana , Pandya Naushira
Published: June 2023

The Medicare Annual Wellness Visit (AWV), which includes comprehensive preventative assessments and screenings, is associated with improved preventative services, including vaccination and cancer screenings. However, the AWV alone does not promote whole-person care. Integrating the AWV within an Age-Friendly Health System (AFHS) contextualizes AWV services within a comprehensive geriatric care framework that integrates the “4Ms” (mentation, medication, mobility, and what matters). This study describes and evaluates quality improvement initiatives to improve the completion of AWV within two different AFHS-recognized health systems (an academic university clinic and a Federally Qualified Health Center). The results from this evaluation present opportunities that other health systems can consider for leveraging electronic health records (EHRs) and enabling services to complete AWVs within a 4Ms framework. The implementation results also suggest an adaptation of the 4Ms assessment schedule for patients with complex chronic conditions who may suffer from multiple comorbidities and cognitive impairment.
Exergames in Older Adult Community Centers and Nursing Homes to Improve Balance and Minimize the Risk of Falls in Older Adults: A Systematic Review and Meta-Analysis
Authors: Leal José Carlos , Belo Vinícius Silva , Santos Ingrid Morselli , Ferreira Rodrigo Vinícius , de Melo Saulo Nascimento , da Silva Eduardo Sérgio
Published: June 2023

There is a substantial gap in our knowledge regarding the efficacy of exergames on the reduction of fall risk in older adults. This systematic review analyzes the findings of clinical trials describing the efficacy of exergames to improve balance or reduce the risk of falls in individuals above 60 years of age who are residents in community centers or nursing homes. We searched Google Scholar, PubMed, and Embase up to January 2023. Initially, 52,294 records were screened. After applying the inclusion and exclusion criteria, 20 studies were included in this systematic review. Meta-analyses revealed statistically significant reductions in the risk of falls and improvements in balance. Exergaming tended to produce positive benefits according to the results obtained using different instruments (TUG, PPA, BBS, and others), control groups, and times of intervention. Nevertheless, a substantial proportion of studies exhibited a high risk of bias and only one had a long follow-up period. Although a large body of evidence supports the view that exergaming is suitable for reducing fall risk and improving balance in older adults, some gaps remain in our knowledge about such benefits.
Victimization and Perception of Abuse in Adolescent and Young Homosexual and Heterosexual Couples in Spain
Authors: Aguilera-Jiménez Noelia , Rodríguez-Franco Luis , Rodríguez-Díaz Francisco Javier , Alameda-Bailén Jose Ramón , Paíno-Quesada Susana G.
Published: June 2023

Currently, violence in adolescent and young couples has a significant social impact on young people’s physical and psychological health. However, the study of violence in homosexual couples must also be addressed. This research analyzes the levels of violent victimization and the perception of abuse in both homosexual and heterosexual couples. Participants’ ages ranged between 14 and 29 years (M = 20.14, SD = 3.464). We used The Dating Violence Questionnaire-Revised (CUIVNO-R), which was applied in two consecutive studies. The results indicate high levels of victimization, especially in the sample of homosexual participants. The scores generally show a low perception of couple violence but high victimization rates. The results of this study reveal the importance of the issue of violence in couples from minority groups and suggest that couple violence should not be understood as unidirectional, i.e., exclusively from men to women. These findings show the need for education in healthy relationships and consideration of different types of couples in these relationships.
Cognitive Training Improves Joint Stiffness Regulation and Function in ACLR Patients Compared to Healthy Controls
Authors: An Yong Woo , Kim Kyung-Min , DiTrani Lobacz Andrea , Baumeister Jochen , Higginson Jill S. , Rosen Jeffrey , Swanik Charles Buz
Published: June 2023

As cognitive function is critical for muscle coordination, cognitive training may also improve neuromuscular control strategy and knee function following an anterior cruciate ligament reconstruction (ACLR). The purpose of this case-control study was to examine the effects of cognitive training on joint stiffness regulation in response to negative visual stimuli and knee function following ACLR. A total of 20 ACLR patients and 20 healthy controls received four weeks of online cognitive training. Executive function, joint stiffness in response to emotionally evocative visual stimuli (neutral, fearful, knee injury related), and knee function outcomes before and after the intervention were compared. Both groups improved executive function following the intervention (p = 0.005). The ACLR group had greater mid-range stiffness in response to fearful (p = 0.024) and injury-related pictures (p = 0.017) than neutral contents before the intervention, while no post-intervention stiffness differences were observed among picture types. The ACLR group showed better single-legged hop for distance after cognitive training (p = 0.047), while the healthy group demonstrated no improvement. Cognitive training enhanced executive function, which may reduce joint stiffness dysregulation in response to emotionally arousing images and improve knee function in ACLR patients, presumably by facilitating neural processing necessary for neuromuscular control.
Social Distress among Cancer Patients: Differential Effects of Risk Factors and Attenuating Role of Culturally Specific Social Support
Authors: Da’ar Omar B. , Jradi Hoda , Alkaiyat Mohammad , Alolayan Ashwaq , Jazieh Abdul Rahman
Published: June 2023

Introduction: We investigated the association between social distress or toxicity and patients’ clinical conditions, demographic characteristics, and social support and networks, and whether this association differs along the distribution of patients’ distress levels. This study included 156 patients treated at King Abdulaziz Medical City, Riyadh, Saudi Arabia. Methods: We used the previously validated Social Toxicity Assessment Tool in Cancer (STAT-C) to assess cancer patients’ distress. We analyzed distress level, the outcome variable of interest, and covariates to show distribution and identify associations. We then used logistic quantile regression for bounded outcomes to assess the association between social distress or toxicity and patients’ clinical conditions, demographic characteristics, and social support and network. As an extension, we examined the interaction between disease status and social support, focusing on the moderating role of social support in attenuating the impact of disease status on social distress. Results: The median age of the patients was 51.2 (SD = 21.4, range 22 to 89), with 48.1% being older than 50 years. Of the 156 cancer patients analyzed, 82 (52.6%) were classified as burdened, and 50% of those with uncontrolled disease status were socially distressed. However, there were more socially distressed patients diagnosed within a year and patients undergoing treatment. There was a greater number of patients who shared their diagnosis with family, colleagues, and neighbors with social distress. The odds of suffering from social distress were higher in younger patients (50 years or younger) than in older patients. Social distress was lower in patients who underwent combined chemotherapy, surgery, and radiation compared with patients who received a single treatment regimen (OR = 0.65, CI, −0.820 to −0.036, p = 0.033). The odds of social distress were 67% higher in patients diagnosed within one year than in patients diagnosed more than one year prior (OR = 1.664, CI, 0.100–0.918, p = 0.015). Patients with uncontrolled disease conditions who shared their diagnosis and treatment with social networks were 48% less likely to experience social distress. Thus, sharing cancer diagnoses with social networks has a statistically significant moderating effect by attenuating the impact of disease status on social distress. Conclusion: Understanding the risk factors for social distress may be important for cancer management. Additionally, identifying the moderating role that patients’ sharing of cancer diagnoses in social networks plays in attenuating the impact of disease status on social distress may provide healthcare providers with valuable insights for holistic culture-specific care.
The Relation between Neonatal Intensive Care Units and Postpartum Post-Traumatic Stress Disorder after Cesarean Section
Authors: Orovou Eirini , Eskitzis Panagiotis , Mrvoljak-Theodoropoulou Irina , Tzitiridou-Chatzopoulou Maria , Dagla Maria , Arampatzi Christiana , Iliadou Maria , Antoniou Evangelia
Published: June 2023

Background: The experience of a neonate hospitalized in the NICU is an understandably traumatic experience for parents, especially for the mothers of neonates. This mental distress resulting from preterm birth and/or NICU hospitalization can be understood as post-traumatic symptomatology, according to the Diagnostic and Statistical Manual of Mental Disorders (DSM-5 version). The aim of this study is to investigate the impact of the admission of a neonate to the NICU (forany reason) on the development of postpartum PTSD in a sample of women after cesarean section. Methods: A total of 469 women who gave birth with cesarean section from July 2019 to June 2020 participated in this study out of the original sample of 490 women who consented to participate. Data were obtained from the researcher’s socio-demographic questionnaire and the post-traumatic stress checklist (PCL-5) from the Diagnostic and Statistical Manual of Mental Disorders DCM-5 version. Results: In total, 11.7% of the sample experienced postpartum PTSD. There is a strong relationship between the inclusion of a neonate to the NICU due to perinatal stress, breathing difficulties, infections, and IUGR with postpartum PTSD (37.7%) in relation to the perinatal stress Criterion A (fear for the life of the neonate), the first criterion of postpartum PTSD. Conclusions: Additional measures must be taken for mothers of children who have been admitted to the neonatal intensive care unit with psychological support interventions and a reassessment of their mental state.
The Barriers to Deprescription in Older Patients: A Survey of Spanish Clinicians
Authors: Mejías-Trueba Marta , Rodríguez-Pérez Aitana , García-Cabrera Emilio , Jiménez-Juan Carlos , Sánchez-Fidalgo Susana
Published: June 2023

Background and objective: There are barriers to deprescription that hinder its implementation in clinical practice. The objective of this study was to analyse the main barriers and limitations of the deprescription process perceived by physicians who care for multipathological patients. Materials and methods: The “deprescription questionnaire of elderly patients” was adapted to an online format and sent to physicians in geriatrics. Question 1 is a reference to establish agreement or disagreement with this practice. The influence of different aspects of deprescription was analysed via the demographic characteristics of the clinicians and perceptions of the various barriers (questions 2–9) by means of bivariate analysis. Based on the latter, a multivariate model was carried out to demonstrate the relationship between barriers and the degree of deprescription agreement among respondents. Results: Of the 72 respondents, 72.2% were in favour of deprescribing. Regarding the analyses, the demographic characteristics did not influence rankings. The deprescription of preventive drugs and consensus with patients were associated with a positive attitude towards deprescribing, while withdrawing drugs prescribed by other professionals, time constraints and patient reluctance emerged as possible barriers. The only factor independently associated with deprescribing was lack of time. Conclusions: Time was found to be the main barrier to deprescription. Training, the creation of multidisciplinary teams and integrated health systems are key facilitators.
Genetic Diversity of 52 Species of Kiwifruit (Actinidia chinensis Planch.)
Authors: Qi Beibei , Wang Faming , Ye Kaiyu , Mo Quanhui , Gong Hongjuan , Liu Pingping , Jiang Qiaosheng , Li Jiewei
Published: June 2023

The genetic diversity of 52 kiwifruit wild germplasms, which were collected by the kiwifruit germplasm nursery of the Guangxi Institute of Botany, was studied using start-codon-targeted (SCoT) molecular markers. The objective was to understand the genetic relationships of the 52 kiwifruit wild germplasms. The results showed that a total of 113 bands were amplified from 52 wild kiwifruit germplasms using 10 primers (9–17 bands per primer, 11.3 on average), of which 103 (91.15%) were polymorphic bands (10.3 bands per primer). The genetic identity of the 52 wild kiwifruit germplasms was in the range of 0.405–1.000, with an average of 0.709. The genetic distance was 0.000–0.904, with an average of 0.355. At the threshold of 0.650, the germplasms were clustered into three clusters. The genetic distances between Guilin and Fushekuoye and between Jinhua and Liangye were both the smallest (0.000), meaning they had a similarity of 100%, as indicated by the SCoT molecular markers. In conclusion, the genetic relationships at the molecular level (genetic distance) were clustered preferentially according to the place of origin instead of by morphological classification, geographical distribution, or ploidy. The genetic relationships between wild kiwifruit germplasms were partially verified through disease resistance analysis.
Streamflow Reconstructions Using Tree-Ring-Based Paleo Proxies for the Sava River Basin (Slovenia)
Authors: Tootle Glenn , Oubeidillah Abdoul , Elliott Emily , Formetta Giuseppe , Bezak Nejc
Published: June 2023

The Sava River Basin (SRB) extends across six countries (Slovenia, Croatia, Bosnia and Herzegovina, Serbia, Albania, and Montenegro) and is a major tributary of the Danube River (DR). The Sava River (SR) originates in the alpine region of Slovenia, and, in support of a Slovenian government initiative to increase clean, sustainable energy, multiple hydropower facilities have been constructed within the past ~20 years. Given the importance of this river system for varying demands, including energy production, information about past (paleo) drought and pluvial periods would provide important information to water managers and planners. Seasonal (April–May–June–July–August–September—AMJJAS) streamflow data were obtained for two SRB gauges (Jesenice and Catez) in Slovenia. The Jesenice gauge is in the extreme headwaters of the SR, upstream of any major water control structures, and is considered an unimpaired (minimal anthropogenic influence) gauge. The Catez gauge is located on the SR near the Slovenia–Croatia border, thus providing an estimate of streamflow leaving Slovenia (entering Croatia). The Old World Drought Atlas (OWDA) provides an annual June–July–August (JJA) self-calibrating Palmer Drought Severity Index (scPDSI) derived from 106 tree-ring chronologies for 5414 grid points across Europe from 0 to 2012 AD. In lieu of tree-ring chronologies, this dataset was used as a proxy to reconstruct (for ~2000 years) seasonal streamflow. Prescreening methods included the correlation and temporal stability of seasonal streamflow and scPDSI cells. The retained scPDSI cells were then used as predictors (independent variables) to reconstruct streamflow (predictive and/or dependent variables) in regression-based models. This resulted in highly skillful reconstructions of SRB seasonal streamflow from 0 to 2012 AD. The reconstructions were evaluated, and both low flow (i.e., drought) and high flow (i.e., pluvial) periods were identified for various filters (5-year to 30-year). When evaluating the most recent ~20 years (2000 to present), multiple low-flow (drought) periods were identified. For various filters (5-year to 15-year), the 2003 end-year consistently ranked as one of the lowest periods, while the 21-year period ending in 2012 was the lowest flow period in the ~2000-year reconstructed-observed-historic period of record. The ~30-year period ending in 2020 was the lowest flow period since the early 6th century. A decrease in pluvial (wet) periods was identified in the observed-historic record when compared to the paleo record, again confirming an apparent decline in streamflow. Given the increased activities (construction of water control structures) impacting the Sava River, the results provide important information to water managers and planners.
Predictive MPC-Based Operation of Urban Drainage Systems Using Input Data-Clustered Artificial Neural Networks Rainfall Forecasting Models
Authors: Jafari Fatemeh , Mousavi S. Jamshid , Ponnambalam Kumaraswamy
Published: June 2023

The model predictive control (MPC) approach can be implemented in either a reactive (RE-) or predictive (PR-) manner to control the operation of urban drainage systems (UDSs). Previous research focused mostly on the RE-MPC, as the PR-MPC, despite its potential to improve the performance of the UDS operations, requires additional computational resources and is more complex. This research evaluates the conditions under which the PR-MPC approach may be preferable. A PR-MPC model is developed, consisting of an adaptive input data-clustered ANN-based rainfall forecasting method coupled to an MPC framework. Observed and forecasted rainfall events are inputs to the internal MPC model, including the rainfall-runoff SWMM simulation model of the system and the MPC optimizer, which is a harmony search-based model determining optimal control policies. The proposed model was used as part of the UDS of Tehran, Iran, under different scenarios of input (rainfall), forecast accuracy (IFAC), and time horizon (IFTH). Results indicate that the PR-MPC performs better for longer-duration rainfall events, while the RE-MPC could be used to control very short storm occurrences. The proposed PR-MPC model can achieve between 85 and 92% of the performance of an ideal model functioning under the premise of perfect, error-free rainfall forecasts for two investigated rainfall events. Additionally, the IFAC can be improved by including rainfall fluctuations over finer temporal resolutions than the forecast horizon as additional predictors.
Text to Causal Knowledge Graph: A Framework to Synthesize Knowledge from Unstructured Business Texts into Causal Graphs
Authors: Gopalakrishnan Seethalakshmi , Chen Victor Zitian , Dou Wenwen , Hahn-Powell Gus , Nedunuri Sreekar , Zadrozny Wlodek
Published: June 2023

This article presents a state-of-the-art system to extract and synthesize causal statements from company reports into a directed causal graph. The extracted information is organized by its relevance to different stakeholder group benefits (customers, employees, investors, and the community/environment). The presented method of synthesizing extracted data into a knowledge graph comprises a framework that can be used for similar tasks in other domains, e.g., medical information. The current work addresses the problem of finding, organizing, and synthesizing a view of the cause-and-effect relationships based on textual data in order to inform and even prescribe the best actions that may affect target business outcomes related to the benefits for different stakeholders (customers, employees, investors, and the community/environment).
Lu-Lu Bond in Lu2@C60 Metallofullerenes
Authors: Zhao Yaoxiao , Shen Wangqiang , Chen Weixing , Lu Xing
Published: June 2023

This study on Lu2@C60 isomers provides insights into the metal–metal bond through the confinement effect of fullerene cages. Density functional theory calculations were used to study the nature of the Lu-Lu bond in two stable endohedral metallofullerenes (EMFs), Lu2@C2v_C60 and Lu2@Ih_C60, both with negative endohedral energy. These two isomers are geometrically connected through a simple Stone–Wales (SW) transformation. The electronic configuration of (Lu2)4+@C604− was also confirmed, leading to the formation of a two-center two-electron (2c–2e) Lu-Lu σ single bond. By comparing the Lu-Lu bonds in Lu2@C60 with those in acknowledged Lu2@C2n, the smaller C60 fullerene compressed the geometry of Lu2 resulting in a much shorter Lu-Lu bond length. However, the Lu-Lu bond strength is slightly weaker in Lu2@C60 than that in large fullerenes, as the Lu-Lu bond in C60 is likely a p-p σ bond with an above the 40% contribution of p orbital and a strong metal–cage interaction. Additionally, the vis-NIR spectra of Lu2@C2v_C60 and Lu2@Ih_C60 were simulated, which could provide valuable information for future experimental studies on Lu-based EMFs.
Predicting the Impact of Climate Change on the Geographical Distribution of Leafhopper, Cicadella viridis in China through the MaxEnt Model
Authors: Wei Xinju , Xu Danping , Zhuo Zhihang
Published: June 2023

Cicadella viridis (Hemiptera: Cicadellidae) is an omnivorous leafhopper that feeds on plant sap. It significantly reduces the yield of agricultural and forestry crops while feeding or ovipositing on the host plant. In recent years, the rapid expansion of C. viridis has posed a serious threat to agricultural and forestry crops. To study the impact of climate change on the geographical distribution of the leafhopper, the maximum entropy (MaxEnt) model and ArcGIS software, combined with 253 geographic distribution records of the pest and 24 environmental variables, were used, for the first time, to predict the potential distribution of C. viridis in China under conditions of climatic change. The results showed that the currently suitable areas for C. viridis are 29.06–43° N, 65.25–85.15° E, and 93.45–128.85° E, with an estimated area of 11,231,423.79 km2, i.e., 11.66% of China. The Loess Plateau, the North China Plain, and the Shandong Peninsula are the main suitable areas. The potential distribution of the leafhopper for the high and medium suitability areas decreased under each climate scenario (except RCP8.5 in the 2090s). Several key variables that have the most significant effect on the distribution of C. viridis were identified, including the mean annual temperature (Bio1), the standard deviation of temperature seasonality (Bio4), the minimum temperature of the coldest month (Bio6), and the precipitation of the coldest quarter (Bio19). Our research provides important guidance for developing effective monitoring and pest control methods for C. viridis, given the predicted challenges of altered pest dynamics related to future climate change.
The Improvement of Left Atrial Function after Twelve Weeks of Supervised Concurrent Training in Patients with Heart Failure with Mid-Range Ejection Fraction: A Pilot Study
Authors: Caminiti Giuseppe , Perrone Marco Alfonso , D’Antoni Valentino , Marazzi Giuseppe , Gismondi Alessandro , Vadalà Sara , Di Biasio Deborah , Manzi Vincenzo , Iellamo Ferdinando , Volterrani Maurizio
Published: June 2023

Left atrial dysfunction is associated with exercise intolerance and poor prognosis in heart failure (HF). The effects of exercise training on atrial function in patients with HF with mid-range ejection fraction (HFmrEF) are unknown. The purpose of the present study was to assess the effects of a supervised concurrent training (SCT) program, lasting 12 weeks, on left atrial function of patients with HFmrEF. The study included 70 stable patients, who were randomly assigned into two groups: SCT with (three sessions/week) or a control (CON) group directed to follow contemporary exercise preventive guidelines at home. Before starting the training program and at 12 weeks, all patients performed an ergometric test, a 6 min walk test, and echocardiography. Between-group comparisons were made by analysis of variance (ANOVA). At 12 weeks, the duration of the ergometric test and distance walked at 6 min walk test presented a significant greater increase in SCT compared to the control (between-group p 0.0001 and p 0.004 respectively). Peak atrial longitudinal strain and conduit strain presented an increase of 29% and 34%, respectively, in the SCT, and were unchanged in CON (between-group p 0.008 and p 0.001, respectively). Peak atrial contraction strain increased by 21% in SCT, with no changes in CON (between-group p 0.002). Left ventricular global longitudinal strain increased significantly in SCT compared to control (between-groups p 0.03). In conclusions, SCT improved left atrial and left ventricular function in HFmrEF. Further studies are needed in order to verify whether these favourable effects of SCT on LA function are sustained and whether they will translate into clinical benefits for patients with HFmrEF.
Circulating Profiles of Serum Proguanylin, S100A12 Protein and Pentraxin 3 as Diagnostic Markers of Ulcerative Colitis
Authors: Kałużna Aleksandra , Jura-Półtorak Agnieszka , Derkacz Alicja , Jaruszowiec Julia , Olczyk Krystyna , Komosinska-Vassev Katarzyna
Published: June 2023

The aim of this research was to investigate potential new biomarkers which could be used in the clinical practice of ulcerative colitis (UC). Given the crucial role of intestinal barrier integrity and inflammation in the pathogenesis of UC, the serum profile of proteins linked to intestinal barrier and pro-inflammatory neutrophil products may be useful in diagnosing and monitoring the activity of the disease. We measured serum levels of proguanylin (pro-GN), S100A12, and pentraxin 3 (PTX3) in 31 patients with UC before and after a year of biological treatment, as well as in 20 healthy individuals. Significant differences in the serum profiles of pro-GN (5.27 vs. 11.35, p < 0.001), S100A12 (39.36 vs. 19.74, p < 0.001) and PTX3 (3197.05 vs. 1608.37, p < 0.001) were observed between pre-treatment patients with UC and healthy individuals. Furthermore, in UC patients prior to treatment, the levels of S100A12 (p < 0.0005; r = 0.628) and PTX3 (p < 0.05; r = 0.371) were correlated with disease activity as measured by the Mayo scale. Following a year of biological treatment with adalimumab, the concentration of pro-GN significantly increased (5.27 vs. 6.68, p < 0.005) in the blood of UC patients, while the level of PTX-3 decreased (3197.05 vs. 1946.4, p < 0.0001). Our study demonstrates the usefulness of pro-GN, S100A12, and PTX3 measurements in diagnosing and monitoring the activity of UC.
Exogenous Ochronosis: Characterizing a Rare Disorder in Skin of Color
Authors: Lazar Michelle , De La Garza Henriette , Vashi Neelam A.
Published: June 2023

Exogenous ochronosis is a rare dyschromia that primarily impacts those with skin of color. It is characterized by blue–black pigmentation and is associated with the long-term application of skin-lightening creams containing hydroquinone. Commonly confused with other dyschromias, the use of skin lightening topicals can cause paradoxical skin darkening in patients with known exogenous ochronosis. This is highly distressing to patients, often worsening the underlying dyschromia and making treatment more difficult. A 10-year retrospective analysis was conducted that revealed 25 patients with exogenous ochronosis. The average patient used a skin lightening cream for 9.2 years, with exogenous ochronosis most commonly arising on the cheeks (68%), forehead (24%), and temples (20%). Furthermore, this study identified that patients with exogenous ochronosis may respond well to treatment with Q-switched Alexandrite laser and microneedling. The incidence of exogenous ochronosis is likely to increase as demographics shift and access to a wide range of over-the-counter topicals becomes more available, both in the United States and worldwide. Therefore, it is imperative to better characterize exogenous ochronosis to identify best treatment practices for all patients.
Caries Severity, Decayed First Permanent Molars and Associated Factors in 6–7 Years Old Schoolchildren Living in Palermo (Southern Italy)
Authors: Pizzo Giuseppe , Matranga Domenica , Maniscalco Laura , Buttacavoli Fortunato , Campus Guglielmo , Giuliana Giovanna
Published: June 2023

To date, there are very few epidemiologic studies on caries disease in 6–7 year old children living in Sicily (Southern Italy). The first permanent molar (FPM) is the most commonly affected tooth in this target population, and a one-unit increase in the number of decayed FPMs is predictive of caries in other teeth and in adulthood. The primary aim of this research is to estimate the prevalence of caries in 6–7 year old schoolchildren living in Palermo and, as a secondary aim, to estimate the prevalence of affected FPMs. It was designed as a cluster cross-sectional survey on 995 children from 16 schools, selected based on their geographical location, in one of the eight city districts. Caries data were recorded using the International Caries Detection and Assessment System for each tooth surface. The relation between socio-economic status, behavioural determinants, and clinical information and the number of teeth with initial caries (IC), moderate caries (MC), or extensive caries (SC) was analysed through the ordinal logistic regression. Among the 995 schoolchildren, 662 (66.5%) had at least one lesion and 742 (74.6%) had FPMs. Of the latter, 238 (32.0%) were affected by IC, 86 (11.6%) were affected by MC, and only 3 (0.4%) were affected by SC. During multivariable analysis, there was evidence of an increased risk of MC and SC related to the deprivation of the district in which the children lived and went to school, as well as to the protective role of parental education and employment. The same significant determinants were found for IC and MC FPMs. The study showed the important role of socio-economic determinants, unhealthy behaviours, and social deprivation related to the increased risk of moderate and extensive caries in 6–7 year old schoolchildren. Investigating this target population is very important, as early development of caries in FPMs may have serious consequences in the prognostics of oral health in an adult.
Comparative Study for Characteristics of Locomotive Syndrome in Patients with Lumbar Stenosis and Adult Spinal Deformity
Authors: Ohba Tetsuro , Goto Go , Oda Kotaro , Tanaka Nobuki , Yokomichi Hiroshi , Haro Hirotaka
Published: June 2023

Introduction: The differential diagnoses of lumbar spinal stenosis (LSS) and adult spinal deformity (ASD) have been demonstrated primarily using sagittal radiographic spinopelvic parameters. However, it is more important to know the differences in the characteristic clinical symptoms to make accurate treatment decisions. Recently, the relationship between spinal disease and Locomotive Syndrome (LS) has been reported. Additionally, the Geriatric Locomotive Function Scale-25 (GLFS-25) was reported to be a useful scale to evaluate disease severity and characteristic clinical symptoms in spinal disease. Methods: Sixty-nine consecutive patients with ASD and 196 patients with LSS who underwent spinal surgery were included. Locomotive dysfunction was evaluated using the GLFS-25 questionnaire and physical performance tests including the two-step test and the stand-up test, measured preoperatively. The correlations between sagittal spinopelvic parameters of ASD and LS were examined. Results: All subjects with lumbar degenerative disease in the present study were diagnosed with LS preoperatively. The severity of LS in patients with LSS and ASD were statistically similar. GLFS-25 scores in the mobility and community domain were similarly poor in both groups. Several scores in the domestic life and self-care domains were significantly worse in the ASD group. Question 20 of the GLFS-25, related to load-bearing tasks and housework, was significantly associated with a large pelvic incidence in ASD patients. Conclusions: Lumbar degenerative disease requiring surgery severely affects the LS of older people. ASD patients had more difficulty with load-bearing tasks and housework such as cleaning the yard, carrying heavy bedding, dressing, and bathing compared to LSS patients.