bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved Prediction of Aerodynamic Loss Propagation as Entropy Rise in Wind Turbines Using Multifidelity Analysis
Authors: Siddappaji Kiran , Turner Mark
Published: May 2022

Several physics-based enhancements are embedded in a low-fidelity general unducted rotor design analysis tool developed, py_BEM, including the local Reynolds number effect, rotational corrections to airfoil polar, stall delay model, high induction factor correction, polar at large angle of attack, exergetic efficiency calculation and momentum-based loss. A wind turbine rotor is analyzed in high fidelity designed from py_BEM using a 3D blade generator. It is a design derived from the NREL Phase VI rotor. Three design variations are analyzed using steady 3D CFD solutions to demonstrate the effect of geometry on aerodynamics. S809 and NACA 2420 airfoil properties are used for calculating the aerodynamic loading. Momentum, vorticity and energy transport are explained in depth and connected to entropy production as a measure of performance loss. KE dissipation downstream of the rotor is shown to be a significant contributor of entropy rise. Wake analysis demonstrates mixing with the free stream flow, which begins after 3 diameters downstream of the rotor and extends to about 25 diameters until the decay is very small. Vorticity dynamics is investigated using a boundary vorticity flux technique to demonstrate the relationship between streamwise vorticity and lift generated in boundary layers. Drag components are accounted as well. It is demonstrated using rothalpy that shaft power is not only torque multiplied by rotational velocity but a viscous power loss term must also be included. A multifidelity analysis of wind turbine aerodynamics is demonstrated by capturing flow physics at several levels.
Sustainable Sewage Sludge Management Technologies Selection Based on Techno-Economic-Environmental Criteria: Case Study of Croatia
Authors: Đurđević Dinko , Žiković Saša , Blecich Paolo
Published: May 2022

The management and disposal of sewage sludge is becoming a growing concern at the global level. In the past, the main goal was to completely eliminate sewage sludge since it was deemed a threat to humans and the environment, but recently different possibilities for energy generation and material recovery are emerging. Existing technologies such as incineration or direct application in agriculture contribute to quantity reduction and nutrient recovery but are unable to fully exploit the potential of sewage sludge within the frameworks of circular economy and bioeconomy. This paper developed a model within the PROMETHEE method, which analyses technologies for the sustainable management of sewage sludge, which could make the most from it. For the empirical part of the study, the Republic of Croatia was used as a country in which sewage sludge is increasing in quantity as a result of recent upgrades and expansions in the wastewater system. Incineration, gasification, anaerobic digestion, and nutrient recovery were analyzed as treatment concepts for the increased amounts of sewage sludge. The model reveals that the best solution is the material recovery of sewage sludge, using the struvite production pathway through analysis of selected criteria.
A Hybrid Landslide Warning Model Coupling Susceptibility Zoning and Precipitation
Authors: Sun Deliang , Gu Qingyu , Wen Haijia , Shi Shuxian , Mi Changlin , Zhang Fengtai
Published: May 2022

Landslides are one of the most severe and common geological hazards in the world. The purpose of this research is to establish a coupled landslide warning model based on random forest susceptibility zoning and precipitation. The 1520 landslide events in Fengjie County, Chongqing, China, before 2016 are taken as research cases. We adapt the random forest model to build a landslide susceptibility model. The antecedent effective precipitation model, based on the fractal relationship, is used to calculate the antecedent effective precipitation in the 10 days before the landslide event. Based on different susceptibility zones, the effective precipitation corresponding to different cumulative frequencies is counted as the threshold, and the threshold is adjusted according to the fitted curve. Finally, according to the daily precipitation, the rain warning levels in susceptibility zones are further adjusted, and the final prewarning model of the susceptibility zoning and precipitation coupling is obtained. The results show that the random forest model has good prediction ability for landslide susceptibility zoning, and the precipitation warning model that couples landslide susceptibility, antecedent effective precipitation, and the daily precipitation threshold has high early warning ability. At the same time, it was found that the precipitation warning model coupled with antecedent effective precipitation and the daily precipitation threshold has more accurate precipitation warning ability than the precipitation warning model coupled with the antecedent effective precipitation only; the coupling of the two can complement each other to better characterize the occurrence of landslides triggered by rainfall. The proposed coupled landslide early warning model based on random forest susceptibility and rainfall inducing factors can provide scientific guidance for landslide early warning and prediction, and improve the manageability of landslide risk.
Mean Leaf Angles Affect Irrigation Efficiency and Physiological Responses of Tropical Species Seedling
Authors: Delgado Luiz Gustavo Martinelli , da Silva Richardson Barbosa Gomes , Gabira Mônica Moreno , Rodrigues Angélica Lino , Simões Danilo , de Almeida Luiz Fernando Rolim , da Silva Magali Ribeiro
Published: May 2022

In forest nurseries, irrigation management becomes more complex as different seedlings of tropical species, with different architectures, are grown close to each other. In this context, there are gaps in knowledge about the physiological responses of species with different mean leaf angles when subjected to different irrigation depths. Thus, this work aimed to analyze whether mean leaf angles affect irrigation efficiency and, consequently, physiological responses of tree seedlings. Six species with different mean leaf angles were submitted to three irrigation depths (6, 9, and 12 mm) applied daily by micro-sprinklers in a completely randomized design in a split plot scheme. The following variables were evaluated: leaf water potential, stomatal conductance, relative water content in the leaf, daily transpiration, leaching fraction, and total dry mass. In tree species seedlings with positive mean leaf angles, smaller irrigation depths are already able to increase leaf water potential, stomatal conductance, leaf relative water content, and transpiration efficiency. In contrast, when the mean leaf angles are negative, it is necessary to apply larger irrigation depths so that seedling physiological responses do not reduce the production of total dry mass.
Brown Algae Fucus vesiculosus in Pasta: Effects on Textural Quality, Cooking Properties, and Sensorial Traits
Authors: Ribeiro Ana Ramalho , Madeira Tiago , Botelho Goreti , Martins Diana , Ferreira Ricardo M. , Silva Artur M. S. , Cardoso Susana M. , Costa Rui
Published: May 2022

Fucus vesiculosus is a brown seaweed rich in iodine, fucoxanthin, and phlorotannins, all known to be bioactive compounds associated with health-promoting events. The enrichment of a staple food such as pasta with seaweed flour, could convey health benefits without changing eating habits. In this work, F. vesiculosus flour (FVF) was incorporated into durum wheat pasta at 1, 5.5, and 10% gradient levels. The pasta enriched with FVF needed additional water during dough formation and required more cooking time, resulting in higher weight gain but also increased cooking loss (observed with 5.5 and 10%). The fracturability of raw pasta decreased for all the FVF pasta, though the cooked firmness and hardness were only affected with the inclusion of 10% FVF. The substitution of wheat semolina with FVF at a 10% level caused an increase in the pasta’s fiber content, which resulted in a more discontinuous protein–matrix structure, as observed at the microscopic level. Untrained consumers were very positive about the overall sensory traits of the pasta with low supplementation levels (1 and 5.5%). About 72% of panelists selected the 1% FVF pasta as their favorite sample. The utilization of FVF in pasta should be targeted at low inclusion levels to cope with the expected texture quality and prevent the impairment of the sensory traits.
Novel Dual-Color Immunochromatographic Assay Based on Chrysanthemum-like Au@polydopamine and Colloidal Gold for Simultaneous Sensitive Detection of Paclobutrazol and Carbofuran in Fruits and Vegetables
Authors: Yin Jiaqi , Yan Yiyong , Zhang Kezhuo , Fu Hui , Lu Min , Zhu Hai , Wei Daixian , Peng Juan , Lai Weihua
Published: May 2022

To ensure food safety and prevent the toxic effects of paclobutrazol (PBZ) and carbofuran (CAR) on humans, a sensitive and rapid method for the detection of PBZ and CAR in fruits and vegetables is required. Herein, a highly sensitive PBZ monoclonal antibody (PBZ mAb) and CAR monoclonal antibody (CAR mAb) with half-inhibitory concentrations (IC50) at 0.77 and 0.82 ng mL−1 were prepared, respectively. We proposed a novel dual-color immunochromatographic assay (ICA) with two test lines (T1 and T2) and an independent control line (C) based on chrysanthemum-like Au@Polydopamine (AuNC@PDA) and colloidal gold (AuNPs) for the simultaneous and sensitive detection of PBZ and CAR with naked-eye detection limits of 10 and 5 μg kg−1, respectively. The limits of detection (LOD) for PBZ and CAR were 0.117 and 0.087 μg kg−1 in orange, 0.109 and 0.056 μg kg−1 in grape, and 0.131 and 0.094 μg kg−1 in cabbage mustard, respectively. The average recoveries of PBZ and CAR in orange, grape, and cabbage mustard were 97.86−102.83%, with coefficients of variation from 8.94 to 11.05. The detection results of this method for 30 samples (orange, grapes, and cabbage mustard) agreed well with those of liquid chromatography–tandem mass spectrometry. The novel dual-color ICA was sensitive, rapid, and accurate for the simultaneous detection of PBZ and CAR in real samples.
High Precisive Prediction of Aflatoxin B1 in Pressing Peanut Oil Using Raman Spectra Combined with Multivariate Data Analysis
Authors: Zhu Chengyun , Jiang Hui , Chen Quansheng
Published: May 2022

This study proposes a label-free rapid detection method for aflatoxin B1 (AFB1) in pressing peanut oil based on Raman spectroscopy technology combined with appropriate chemometric methods. A DXR laser Raman spectrometer was used to acquire the Raman spectra of the pressed peanut oil samples, and the obtained spectra were preprocessed by wavelet transform (WT) combined with adaptive iteratively reweighted penalized least squares (airPLS). The competitive adaptive reweighted sampling (CARS) method was used to optimize the characteristic bands of the Raman spectra pretreated by the WT + airPLS, and a partial least squares (PLS) detection model for the AFB1 content was established based on the features optimized. The results obtained showed that the root mean square error of prediction (RMSEP) and determination coefficient of prediction (RP2) of the optimal CARS-PLS model in the prediction set were 22.6 µg/kg and 0.99, respectively. The results demonstrate that the Raman spectroscopy combined with appropriate chemometrics can be used to quickly detect the safety of edible oil with high precision. The overall results can provide a technical basis and method reference for the design and development of the portable Raman spectroscopy system for the quality and safety detection of edible oil storage, and also provide a green tool for fast on-site analysis for regulatory authorities of edible oil and production enterprises of edible oil.
Existence and Stability Results for a Tripled System of the Caputo Type with Multi-Point and Integral Boundary Conditions
Authors: Manigandan Murugesan , Subramanian Muthaiah , Nandha Gopal Thangaraj , Unyong Bundit
Published: May 2022

In this paper, we introduce and investigate the existence and stability of a tripled system of sequential fractional differential equations (SFDEs) with multi-point and integral boundary conditions. The existence and uniqueness of the solutions are established by the principle of Banach’s contraction and the alternative of Leray–Schauder. The stability of the Hyer–Ulam solutions are investigated. A few examples are provided to identify the major results.
Self-Assembled Peptide Habitats to Model Tumor Metastasis
Authors: Al Balushi Noora , Boyd-Moss Mitchell , Samarasinghe Rasika M. , Rifai Aaqil , Franks Stephanie J. , Firipis Kate , Long Benjamin M. , Darby Ian A. , Nisbet David R. , Pouniotis Dodie , Williams Richard J.
Published: May 2022

Metastatic tumours are complex ecosystems; a community of multiple cell types, including cancerous cells, fibroblasts, and immune cells that exist within a supportive and specific microenvironment. The interplay of these cells, together with tissue specific chemical, structural and temporal signals within a three-dimensional (3D) habitat, direct tumour cell behavior, a subtlety that can be easily lost in 2D tissue culture. Here, we investigate a significantly improved tool, consisting of a novel matrix of functionally programmed peptide sequences, self-assembled into a scaffold to enable the growth and the migration of multicellular lung tumour spheroids, as proof-of-concept. This 3D functional model aims to mimic the biological, chemical, and contextual cues of an in vivo tumor more closely than a typically used, unstructured hydrogel, allowing spatial and temporal activity modelling. This approach shows promise as a cancer model, enhancing current understandings of how tumours progress and spread over time within their microenvironment.
Empirical Evidence on Enhanced Mutation Rates of 19 RM-YSTRs for Differentiating Paternal Lineages
Authors: Javed Faqeeha , Shafique Muhammad , McNevin Dennis , Javed Muhammad Usama , Shehzadi Abida , Shahid Ahmad Ali
Published: May 2022

Rapidly mutating Y-chromosomal short tandem repeats (RM Y STRs) with mutation rates ≥ 10−2 per locus per generation are valuable for differentiating amongst male paternal relatives where standard Y STRs with mutation rates of ≤10−3 per locus per generation may not. Although the 13 RM Y STRs commonly found in commercial assays provide higher levels of paternal lineage differentiation than conventional Y STRs, there are many male paternal relatives that still cannot be differentiated. This can be improved by increasing the number of Y STRs or choosing those with high mutation rates. We present a RM Y STR multiplex comprising 19 loci with high mutation rates and its developmental validation (repeatability, sensitivity and male specificity). The multiplex was found to be robust, reproducible, specific and sensitive enough to generate DNA profiles from samples with inhibitors. It was also able to detect all contributor alleles of mixtures in ratios up to 9:1. We provide preliminary evidence for the ability of the multiplex to discriminate between male paternal relatives by analyzing large numbers of male relative pairs (536) separated by one to seven meioses. A total of 96 mutations were observed in 162 meioses of father–son pairs, and other closely related male pairs were able to be differentiated after 1, 2, 3, 4, 5, 6 and 7 meiosis in 44%, 69%, 68%, 85%, 0%, 100% and 100% of cases, respectively. The multiplex offers a noticeable enhancement in the ability to differentiate paternally related males compared with the 13 RM Y STR set. We envision the future application of our 19 RM Yplex in criminal cases for the exclusion of male relatives possessing matching standard Y STR profiles and in familial searching with unknown suspects. It represents a step towards the complete individualization of closely related males.
Genetic Expression between Ageing and Exercise: Secreted Protein Acidic and Rich in Cysteine as a Potential “Exercise Substitute” Antiageing Therapy
Authors: Ghanemi Abdelaziz , Yoshioka Mayumi , St-Amand Jonny
Published: May 2022

Ageing is the effect of time on biological entities. It represents a risk factor for a variety of diseases and health disorders; thus, therapeutic options are required to tackle ageing issues. Modern geriatric medicine prescribes exercise to counteract ageing effects. This work presents secreted protein acidic and rich in cysteine (SPARC) as a potential antiageing therapy. Indeed, SPARC declines with ageing, exercise induces SPARC, and SPARC overexpression in mice mimics exercise. Thus, we hypothesize that SPARC is an exercise-induced factor that is beyond—at least part of—the antiageing effects induced by exercise. This could become a potential antiageing therapy for the elderly that counteracts ageing by mimicking the effects of exercise without needing to perform exercise. This is of particular importance because ageing usually reduces mobility and age-related diseases can reduce the ability to perform the required physical activity. On the other hand, the possibilities of mimicking exercise benefits via SPARC are not limited to ageing, and can be applied in various contexts in which exercise cannot be performed because of physical disabilities, health disorders, or limited mobility.
Construction and Validation of a Tumor Microenvironment-Based Scoring System to Evaluate Prognosis and Response to Immune Checkpoint Inhibitor Therapy in Lung Adenocarcinoma Patients
Authors: Huang Pinzheng , Xu Linfeng , Jin Mingming , Li Lixi , Ke Yizhong , Zhang Min , Zhang Kairui , Lu Kongyao , Huang Gang
Published: May 2022

Background: Lung cancer is among the most dangerous malignant tumors to human health. Lung adenocarcinoma (LUAD) accounts for about 40% of all lung cancers. Accumulating evidence suggests that the tumor microenvironment (TME) is a crucial regulator of carcinogenesis and therapeutic efficacy in LUAD. However, the impact of tumor microenvironment-related signatures (TMERSs) representing the TME characteristics on the prognosis and therapeutic outcome of LUAD patients remains to be further explored. Materials and methods: Gene expression files and clinical information of 1630 LUAD samples and 275 samples with immunotherapy information from different databases such as The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and Cancer Research Institute (CRI) iAtlas were downloaded and analyzed. Three hundred tumor microenvironment-related signatures (TMERS) based on a comprehensive collection of marker genes were quantified by single sample gene set enrichment analysis (ssGSEA), and then eight significant signatures were selected to construct the tumor microenvironment-related signature score (TMERSscore) by performing Least Absolute Shrinkage and Selection Operator (LASSO)-Cox analysis. Results: In this study, we constructed a TME-based prognostic stratification model for patients with LUAD and validated it in several external datasets. Furthermore, the TMERSscore was found to be positively correlated with tumor malignancy and a high TMERSscore predicted a poor prognosis. Moreover, the TMERSscore of responders treated with Immune Checkpoint Inhibitor (ICI) therapies was significantly lower than that of non-responders, and the TMERSscore was positively correlated with the tumor immune dysfunction and exclusion (TIDE) score, implying that a low TMERSscore predicts a better response to ICI treatment and may provide independent and incremental predictive value over current biomarkers. Conclusions: Overall, we constructed a TMERSscore that can be used for LUAD patient prognosis stratification as well as ICI therapeutic efficacy evaluation, supportive results from independent external validation sets showed its robustness and effectiveness.
Transcriptomics Integrated with Metabolomics Unveil Carotenoids Accumulation and Correlated Gene Regulation in White and Yellow-Fleshed Turnip (Brassica rapa ssp. rapa)
Authors: Ren Yanjing , Han Rui , Ma Yidong , Li Xiaojuan , Deng Changrong , Zhao Mengliang , Li Jiang , Hou Quangang , Zhong Qiwen , Shao Dengkui
Published: May 2022

Turnip (Brassica rapa ssp. rapa) is considered to be a highly nutritious and health-promoting vegetable crop, whose flesh color can be divided into yellow and white. It is widely accepted that yellow-fleshed turnips have higher nutritional value. However, reports about flesh color formation is lacking. Here, the white-fleshed inbred line, W21, and yellow-fleshed inbred line, W25, were profiled from the swollen root of the turnip at three developmental periods to elucidate the yellow color formation. Transcriptomics integrated with metabolomics analysis showed that the PSY gene was the key gene affecting the carotenoids formation in W25. The coding sequence of BrrPSY-W25 was 1278 bp and that of BrrPSY-W21 was 1275 bp, and BrrPSY was more highly expressed in swollen roots in W25 than in W21. Transient transgenic tobacco leaf over-expressing BrrPSY-W and BrrPSY-Y showed higher transcript levels and carotenoids contents. Results revealed that yellow turnip formation is due to high expression of the PSY gene rather than mutations in the PSY gene, indicating that a post-transcriptional regulatory mechanism may affect carotenoids formation. Results obtained in this study will be helpful for explaining the carotenoids accumulation of turnips.
Application of Electron Backscatter Diffraction to Calcite-Twinning Paleopiezometry
Authors: Rutter Ernest , Wallis David , Kosiorek Kamil
Published: May 2022

Electron backscatter diffraction (EBSD) was used to determine the orientation of mechanically twinned grains in Carrara marble experimentally deformed to a small strain (≤4%) at room temperature and at a moderate confining pressure (225 MPa). The thicknesses of deformation twins were mostly too small to permit determination of their orientation by EBSD but it proved possible to measure their orientations by calculating possible twin orientations from host grain orientation, then comparing calculated traces to the observed twin traces. The validity of the Turner & Weiss method for principal stress orientations was confirmed, particularly when based on calculation of resolved shear stress. Methods of paleopiezometry based on twinned volume fraction were rejected but a practical approach is explored based on twin density. However, although twin density correlates positively with resolved shear stress, there is intrinsic variability due to unconstrained variables such as non-uniform availability of twin nucleation sites around grain boundaries that imposes a limit on the achievable accuracy of this approach.
Dynamic Modeling of Inland Flooding and Storm Surge on Coastal Cities under Climate Change Scenarios: Transportation Infrastructure Impacts in Norfolk, Virginia USA as a Case Study
Authors: Shen Yawen , Tahvildari Navid , Morsy Mohamed M. , Huxley Chris , Chen T. Donna , Goodall Jonathan Lee
Published: May 2022

Low-lying coastal cities across the world are vulnerable to the combined impact of rainfall and storm tide. However, existing approaches lack the ability to model the combined effect of these flood mechanisms, especially under climate change and sea level rise (SLR). Thus, to increase flood resilience of coastal cities, modeling techniques to improve the understanding and prediction of the combined effect of these flood hazards are critical. To address this need, this study presents a modeling system for assessing the combined flood impact on coastal cities under selected future climate scenarios that leverages ocean modeling with land surface modeling capable of resolving urban drainage infrastructure within the city. The modeling approach is demonstrated in quantifying the impact of possible future climate scenarios on transportation infrastructure within Norfolk, Virginia, USA. A series of combined storm events are modeled for current (2020) and projected future (2070) climate scenarios. The results show that pluvial flooding causes a larger interruption to the transportation network compared to tidal flooding under current climate conditions. By 2070, however, tidal flooding will be the dominant flooding mechanism with even nuisance flooding expected to happen daily due to SLR. In 2070, nuisance flooding is expected to cause a 4.6% total link close time (TLC), which is more than two times that of a 50-year storm surge (1.8% TLC) in 2020. The coupled flood model was compared with a widely used but physically simplistic bathtub method to assess the difference resulting from the more complex modeling presented in this study. The results show that the bathtub method overestimated the flooded area near the shoreline by 9.5% and 3.1% for a 10-year storm surge event in 2020 and 2070, respectively, but underestimated the flooded area in the inland region by 9.0% and 4.0% for the same events. The findings demonstrate the benefit of sophisticated modeling methods compared to more simplistic bathtub approaches, in climate adaptive planning and policy in coastal communities.
Persistent RNA SARS-CoV-2 Detection in a HIV-Infected Patient
Authors: Giubelan Lucian , Stanciu Ilona , Ilie Cristina , Pădureanu Vlad
Published: May 2022

The aim of this paper is to present a case of COVID-19 in a newly diagnosed HIV-infected, severely immunodepressed patient with a long persistence of positive RT-PCR for RNA SARS-CoV-2. Indirect data suggests that viable virus persisted for a long time in the absence of an adequate defense of the host. Improved immunity after starting antiretroviral treatment was not associated with an increased inflammatory reaction as regarding the infection due to the coronavirus and, apparently, was the main factor to control the infection. Remdesivir used to combat the SARS-CoV-2 infection had no immediate effect on the recorded cycle-threshold.
Evaluation of the Psychometric Properties of the Revised Piper Fatigue Scale in Patients with Multiple Sclerosis
Authors: Berardi Anna , Graziosi Giulia , Ferrazzano Gina , Casagrande Conti Laura , Grasso Maria Grazia , Tramontano Marco , Conte Antonella , Galeoto Giovanni
Published: May 2022

Background: Fatigue is one of the most common symptoms in patients with multiple sclerosis (MS), and has a major impact on their quality of life. Measurement tools that assess the patient’s condition are commonly used in the neurological field, though diagnostic tools are currently unable to distinguish potential alternative causes of fatigue in individual patients. The Revised Piper Fatigue Scale (PFS-R) is a self-administered assessment scale that is internationally used for fatigue measurement. Aim: This study aimed to evaluate the reliability and validity of the Italian version of the PFS-R in patients with MS. Methods: Forty-one individuals were included in this study. Each participant in the study was given a dossier, in paper format, containing the informed consent form, a personal data sheet, the Fatigue Symptoms and Impacts Questionnaire in Relapsing Multiple Sclerosis (FSIQ-RMS), the Fatigue Severity Scale (FSS), and the PFS-R. Results: The PFS-R was found to have strong internal consistency, with a value of α equal to 0.977. Correlations between PFS-R, FSS, and FSIQ-RMS scores were analyzed using the Pearson correlation coefficient, and all scales showed statistically significant correlations. Conclusion: The PFS-R is a new self-administered tool to assess fatigue in patients with MS. It evaluates fatigue characteristics, difficulty in carrying out daily life activities, and how the individual feels as a result of this symptom. This tool was previously validated for use in cancer patients, which also allows us to make a comparison between different pathologies and rehabilitation treatments.
Pneumonia Transfer Learning Deep Learning Model from Segmented X-rays
Authors: Alharbi Amal H. , Hosni Mahmoud Hanan A.
Published: May 2022

Pneumonia is a common disease that occurs in many countries, more specifically, in poor countries. This disease is an obstructive pneumonia which has the same impression on pulmonary radiographs as other pulmonary diseases, which makes it hard to distinguish even for medical radiologists. Lately, image processing and deep learning models are established to rapidly and precisely diagnose pneumonia disease. In this research, we have predicted pneumonia diseases dependably from the X-ray images, employing image segmentation and machine learning models. A public labelled database is utilized with 4000 pneumonia disease X-rays and 4000 healthy X-rays. ImgNet and SqueezeNet are utilized for transfer learning from their previous computed weights. The proposed deep learning models are trained for classifying pneumonia and non-pneumonia cases. The following processes are presented in this paper: X-ray segmentation utilizing BoxENet architecture, X-ray classification utilizing the segmented chest images. We propose the improved BoxENet model by incorporating transfer learning from both ImgNet and SqueezeNet using a majority fusion model. Performance metrics such as accuracy, specificity, sensitivity and Dice are evaluated. The proposed Improved BoxENet model outperforms the other models in binary and multi-classification models. Additionally, the Improved BoxENet has higher speed compared to other models in both training and classification.
Effect of CaCl2 Treatment on Enzymatic Browning of Fresh-Cut Luffa (Luffa cylindrica)
Authors: Feng Yingna , Feng Cui , Wang Yuanhua , Gao Shuai , Sun Pengpeng , Yan Zhiming , Su Xiaojun , Sun Ying , Zhu Qianqian
Published: May 2022

Enzymatic browning is a major issue that reduces the commercial value of Luffa cylindrica during storage, processing, and transportation. Our results showed that 1% CaCl2 treatment was optimal for reducing the surface browning of fresh-cut luffa. After storage at 25 °C for four days, the treated luffa had a significantly higher total phenolic (TP) content than the untreated luffa. At the end of the storage period, the calcium treatment showed low malondialdehyde (MDA) and hydrogen peroxide (H2O2) accumulation in the luffa. The treated luffa maintained higher superoxide dismutase (SOD), catalase (CAT), and phenylalanine ammonia lyase (PAL) activities and lower polyphenol oxidase (PPO) activity as compared to the untreated luffa. Furthermore, the genes regulating SOD (e.g., SOD1, SOD2, and SOD3), CAT (e.g., LcCAT1 and CAT2), and PAL (e.g., PAL1 and PAL2) in calcium-treated luffa were upregulated to varying degrees, suggesting that Ca2+ inhibited the browning of fresh-cut tissue by regulating the activities of those enzymes. Ultrastructure images showed that the treated luffa could maintain the relative integrity of the cell membrane and organelles. Therefore, Ca2+ might act as a second messenger to reduce ROS oxidative damage and maintain the cell membrane integrity. This study provides new insights into the breeding of new luffa varieties that are resistant to browning and post-harvest treatments to reduce the browning of luffa tissue.
Can the Adjustment and Renovation Policies of Old Industrial Cities Reduce Urban Carbon Emissions?—Empirical Analysis Based on Quasi-Natural Experiments
Authors: Zhang Rongbo , Zhong Changbiao
Published: May 2022

Based on a literature review and theoretical mechanism, this paper takes the implementation point of the adjustment and transformation policy for old industrial cities as the breakthrough point, and uses a regression model to explore the impact of the adjustment and transformation policy of these old industrial cities on urban carbon emissions. This paper also robustly tests the effective mechanisms and environmental hypotheses. Overall, the implementation of the adjustment and renovation policy has significantly reduced the carbon emissions of old industrial cities by about 0.068 units. Compared with the control group cities, the pilot cities reduced carbon emissions by an average of about 310,000 tons after the implementation of the policy. Based on a summary of the excellent Chinese case experience and an empirical analysis, it can be concluded that improvements in the green innovation capacity of old industrial cities, the agglomeration of high-end service industries, and the strengthening of ecological restoration are important mechanisms that lead to reduced carbon emissions. There is no subsequent exacerbation of the carbon intensity of neighboring cities, and there is insufficient evidence to prove pollution via neighboring transfers and use of the beggar-thy-neighbor policy. The extended analysis shows that the “inverted U-shaped” CO2 Kuznets environmental curve hypothesis is significantly present in the sample of old industrial cities, but most cities do not cross the threshold. In 2013, about 60% of the urban sample economic growth and carbon emissions showed signs of tapping into potentials and increasing efficiency (absolute decoupling) and intensive expansion (relative decoupling). In old industrial cities, the proportion of relative decoupling shows a fluctuating upward trend. In the future, the government should accurately select its own development orientation and actively seek the “best balance” between economic growth and a green and low-carbon path.
Adverse Events in Isotretinoin Therapy: A Single-Arm Meta-Analysis
Authors: Kapała Jan , Lewandowska Julia , Placek Waldemar , Owczarczyk-Saczonek Agnieszka
Published: May 2022

Isotretinoin (ISO) is an oral prescription-only retinoid, well known for its acne-treating effect. However, it affects a substantial number of human cell types, causing a broad spectrum of adverse effects. The purpose of this study is to establish the isotretinoin therapy adverse events among human clinical trials and their prevalence. Two authors (J.K., J.L.) systematically performed the literature review and assessment from December 2021–February 2022. Three databases (PubMed, ClinicalTrials, and Cochrane Library) were searched using the following terms: “isotretinoin acne vulgaris” for published studies in English from 1980–2021. Finally, 25 randomized controlled clinical trials (RCTs) and five open-label clinical trials provided 3274 acne vulgaris suffering patients. Isotretinoin therapy affects almost all of the systems in the human body, causing numerous adverse events. However, they mainly concern mild mucocutaneous conditions (severe cases are rare) and represent individual responses to a drug. In addition, all adverse events are reversible and can be avoided by specific preparations.
Adipokines Level and Cognitive Function—Disturbance in Homeostasis in Older People with Poorly Managed Hypertension: A Pilot Study
Authors: Kujawska Agnieszka , Kujawski Sławomir , Kozakiewicz Mariusz , Hajec Weronika , Kwiatkowska Małgorzata , Skierkowska Natalia , Husejko Jakub , Newton Julia L. , Zalewski Paweł , Kędziora-Kornatowska Kornelia
Published: May 2022

Aim: To explore the network relationship between cognitive function, depressive symptom intensity, body composition, proxies of cognitive reserve, trophic factor, adipokines and myokines, physical performance and blood pressure in a group of older people with poorly managed hypertension (PMHTN) compared to a normotensive (NTN) group. Materials and methods: History of hypertension and blood pressure level were examined in older participants. Thirty-one subjects diagnosed with PMHTN (history of hypertension diagnosis and values of sBP or dBP over 140/90 mmHg) and eighteen NTN (lack of history of hypertension and sBP and dBP lower than 140/90 mmHg) participated. Participants completed physical and cognitive function assessments: including the Mini–Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA) and its two subtests Delayed Recall (DR) and Verbal Fluency (VF) and Trail Making Test Part B (TMT B). Factors associated with cognitive functioning: age, years of education, cognitive and travel activity were assessed using a questionnaire. Visceral fat was determined by bioimpedance testing and gait velocity and agility assessed using an Up and Go test. To summarize the strength and direction (negative or positive) of a relationship between two variables, Spearman’s rank correlation coefficient was used. Then, network graphs were created to illustrate the relationship between variables. Node strength (number of edges per node), neighbourhood connectivity (the average connectivity of all the neighbours of a node), stress (the number of shortest paths passing through each node) were compared in network from PMHTN group to network from NTN group. Results: Neighbourhood connectivity and stress were significantly higher in of the PMHTN network compared to NTN (6.03 ± 1.5 vs. 4.23 ± 2.5, p = 0.005 and 118.21 ± 137.6 vs. 56.87 ± 101.5, p = 0.02, accordingly). Conclusion: In older subjects with poorly managed hypertension, dyshomeostasis was observed, compared to normotensive subjects.
Emergency Craniotomy and Burr-Hole Trephination in a Low-Resource Setting: Capacity Building at a Regional Hospital in Cambodia
Authors: Hu Jingjing , Sokh Vannara , Nguon Sophy , Heng Yang Van , Husum Hans , Kloster Roar , Odland Jon Øyvind , Xu Shanshan
Published: May 2022

To evaluate the teaching effect of a trauma training program in emergency cranial neurosurgery in Cambodia on surgical outcomes for patients with traumatic brain injury (TBI). We analyzed the data of TBI patients who received emergency burr-hole trephination or craniotomy from a prospective, descriptive cohort study at the Military Region 5 Hospital between January 2015 and December 2016. TBI patients who underwent emergency cranial neurosurgery were primarily young men, with acute epidural hematoma (EDH) and acute subdural hematoma (SDH) as the most common diagnoses and with long transfer delay. The incidence of favorable outcomes three months after chronic intracranial hematoma, acute SDH, acute EDH, and acute intracerebral hematoma were 96.28%, 89.2%, 93%, and 97.1%, respectively. Severe traumatic brain injury was associated with long-term unfavorable outcomes (Glasgow Outcome Scale of 1–3) (OR = 23.9, 95% CI: 3.1–184.4). Surgical outcomes at 3 months appeared acceptable. This program in emergency cranial neurosurgery was successful in the study hospital, as evidenced by the fact that the relevant surgical capacity of the regional hospital increased from zero to an acceptable level.
Pulse Wave Velocity and Sarcopenia in Older Persons—A Systematic Review and Meta-Analysis
Authors: Piotrowicz Karolina , Klich-Rączka Alicja , Skalska Anna , Gryglewska Barbara , Grodzicki Tomasz , Gąsowski Jerzy
Published: May 2022

Sarcopenia and cardiovascular disease share some of the pathophysiologic mechanisms. Sarcopenia is likewise an important feature of frailty and the one potentially related to cardiovascular pathology. Previously, the relationship between arterial stiffness and frailty has been established. In this study, we conducted a systematic review and a meta-analysis of studies where the relationship between pulse wave velocity (PWV) and sarcopenia has been addressed. We included six cross-sectional studies that enrolled 5476 participants. Using the WebPlotDigitizer, RevMan5, and SAS 9.4, we extracted or calculated the summary statistics. We then calculated standardized mean differences (SMD) of PWV in the sarcopenic and non-sarcopenic participants. The pooled SMD was 0.73 (95% CI 0.39–1.08, p < 0.0001, I2 = 90%) indicating higher value in the sarcopenic subjects. The three studies that presented odds ratios for sarcopenia as a function of PWV homogenously indicated a greater probability of concomitant sarcopenia with higher values of PWV. Greater stiffness of the aorta is associated with sarcopenia. It is impossible to establish the causation. However, the plausible explanation is that increased stiffness may translate into or be an intermediary phenotype of common vascular and muscle damage. On the other hand, sarcopenia, which shares some of the inflammatory mechanisms with cardiovascular disease, may wind up the age-related large arterial remodeling.
The Use of Static Posturography Cut-Off Scores to Identify the Risk of Falling in Older Adults
Authors: Wiśniowska-Szurlej Agnieszka , Ćwirlej-Sozańska Agnieszka , Wilmowska-Pietruszyńska Anna , Sozański Bernard
Published: May 2022

Background: Falling is the most common accident that occurs in daily living and the second leading cause of unintentional injury death worldwide. The complexity of the risk factors associated with falling makes older people at risk of falling difficult to identify. The aim of the study was to identify the cut-off scores of standing posturography measures that can be used to predict the risk of falling in older adults. Methods: This observational study involved 267 elderly people aged 65 to 85 years (73.99 SD 7.51) living in south-eastern Poland. The subjects were divided into two groups: a group with a high risk of falling and a group with a low risk of falling, based on their timed up-and-go test. Postural stability was assessed during eyes-open and eyes-closed trials using the two-plate stability platform CQ Stab 2P. Results: The best accuracy, sensitivity, and specificity were observed for the sway path, anterior-posterior sway path, and medial-lateral sway path with open and closed eyes. The clinical cut-off score to predict the risk of falling was 350.63 for the sway path with open eyes, 272.64 for the anterior-posterior sway path, and 159.63 for the medial-lateral sway path. The clinical cut-off score for sway path with closed eyes was 436.11. Conclusions: Static posturography screenings in clinical practice may also be useful for detecting typical balance changes in older adults.
Effects of Commercial Probiotics on Colonic Sensitivity after Acute Mucosal Irritation
Authors: López-Gómez Laura , Antón Jaime , López-Tofiño Yolanda , Pomana Bianca , Uranga José A. , Abalo Raquel
Published: May 2022

Gastrointestinal pathologies associated with abdominal pain, such as irritable bowel syndrome or inflammatory bowel disease, lack sufficiently effective treatments. In our study we have used a rat model of visceral pain (72 animals; n = 8–13 per experimental group) to analyze the consequences of intracolonic administration of the irritant acetic acid on visceral sensitivity, histology of the colonic wall, and inflammatory response. Moreover, we have studied the possible beneficial effects of a pretreatment with a commercial probiotic (Actimel®). Contrary to expectations, acetic acid application (7 cm proximal to the anus) decreased the nociceptive response to intracolonic mechanical stimulation, with a slight increase in the histological damage of colonic mucosa. The intensity of these changes depended on the concentration (4% or 0.6%) and the time of application (30 or 60 min). Pretreatment with probiotics (by daily gavage, for 1 week) normalized the values obtained in the visceral sensitivity test but revealed an increase in the number of macrophages. These results suggest a possible activation of inhibitory mechanisms early after colonic irritation, not previously described (which need further experimental confirmation), and the ability of probiotics to normalize the effects of acetic acid. In addition, pretreatment with probiotics has a direct effect on immune functions, stimulating macrophagic activity.
The Short-Term Reliability of the Conceptualised ‘Combat Readiness Assessment’
Authors: Moore Daniel , Tayler Martin , Moore Stephen
Published: May 2022

Military fitness testing has historically assessed individual fitness components. Fitness assessments of this nature do not adequately monitor the physical requirements of military operations. The development of a more combat-specific fitness test would enhance accuracy in assessing the soldiers’ readiness for the demands of war. This study aimed to assess the short-term reliability of the conceptualised ‘Combat Readiness Assessment’ (CRA) following a single familiarisation trial with 21 male phase-two British Army Royal Electrical and Mechanical Engineer (REME) recruits (age (years) 19.7 ± 2.5) split into two groups (N = 11 and 10) to conform with recruit availability. The CRA was designed to be a multifaceted fitness assessment aimed at replicating the physical demands of a combat situation for military personnel. Methods: Three repeated assessments of the CRA were completed over a 10-day period (trial one as a familiarisation) to assess the short-term reliability of the CRA post familiarisation. The CRA was completed carrying a 4 kg rifle (SA80 A2) and involved a 400 m (M) run wearing an 11 kg backpack (removed after the 400 M) followed by weighted carries, sprints, casualty drags and agility tasks. Completion time (seconds) was recorded to assess performance. Intraclass correlations (ICCs) (2,1) with 95% confidence intervals (CI), the standard error of the measurement (SEM) and the coefficient of variation (CV) were calculated for completion time for trials 1-3 (T1-3) and 2-3 (T2-3) to assess reliability post-familiarisation. Mean Absolute Percentage Error (MAPE) was calculated for T1-2 and T2-3. Descriptive statistics were calculated for completion time for T1-3 and T2-3. Results: The reliability following a familiarisation trial from T1-3 (ICC: 0.75; SEM: 7.1 s; CV: 9.97%) to T2-3 (ICC: 0.88; SEM: 6.4 s; CV: 10.05%) increased. Mean trial time decreased post familiarisation from T1-3 (210.9 ± 21.03 s) to T2-3 (206.3 ± 20.73 s). Conclusions: These findings are inconclusive regarding the short-term reliability of the CRA. The small sample size resulted in wide 95% confidence intervals associated with the ICCs, making the true ICC value hard to determine. The ICCs and MAPE suggest that the reliability of the CRA increased following a familiarisation trial, but this requires further research with a larger cohort to determine with confidence.