bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization and Activity Analyses of the FLOWERING LOCUS T Promoter in Gossypium Hirsutum
Authors: Sang Na , Cai Darun , Li Chao , Sun Yuqiang , Huang Xianzhong
Published: September 2019

Flowering transition is a crucial development process in cotton (Gossypium hirsutum L.), and the flowering time is closely correlated with the timing of FLOWERING LOCUS T (FT) expression. However, the mechanism underlying the coordination of various cis-regulatory elements in the FT promoter of cotton has not been determined. In this study, a 5.9-kb promoter of FT was identified from cotton. A bioinformatics analysis showed that multiple insertion–deletion sites existed in the 5.9-kb promoter. Different expression levels of a reporter gene, and the induction by sequential deletions in GhFT promoter, demonstrated that 1.8-kb of the GhFT promoter was stronger than 4.2-, 4.8-, and 5.9-kb promoter fragments. The binding sites of the CONSTANS (CO) and NUCLEAR FACTOR Y transcription factors were located within the 1.0-kb sequence upstream of the FT transcription start site. A large number of repeat segments were identified in proximal promoter regions (−1.1 to −1.4 kb). A complementation analysis of deletion constructs between 1.0 and 1.8 kb of G. hirsutum, Gossypium arboretum, and Gossypium raimondii FT promoters revealed that the 1.0-kb fragment significantly rescued the late-flowering phenotype of the Arabidopsis FT loss-of-function mutant ft-10, whereas the 1.8-kb promoter only slightly rescued the late-flowering phenotype. Furthermore, the conserved CORE motif in the cotton FT promoter is an atypical TGTG(N2-3)ATG, but the number of arbitrary bases between TGTG and ATG is uncertain. Thus, the proximal FT promoter region might play an important role affecting the activity levels of FT promoters in cotton flowering.
Use of Modified Clostridium perfringens Enterotoxin Fragments for Claudin Targeting in Liver and Skin Cells
Authors: Beier Laura-Sophie , Rossa Jan , Woodhouse Stephen , Bergmann Sophia , Kramer Holger , Protze Jonas , Eichner Miriam , Piontek Anna , Vidal-y-Sy Sabine , Brandner Johanna , Krause Gerd , Zitzmann Nicole , Piontek Jörg
Published: September 2019

Claudins regulate paracellular permeability in different tissues. The claudin-binding domain of Clostridium perfringens enterotoxin (cCPE) is a known modulator of a claudin subset. However, it does not efficiently bind to claudin-1 (Cldn1). Cldn1 is a pharmacological target since it is (i) an essential co-receptor for hepatitis C virus (HCV) infections and (ii) a key element of the epidermal barrier limiting drug delivery. In this study, we investigated the potential of a Cldn1-binding cCPE mutant (i) to inhibit HCV entry into hepatocytes and (ii) to open the epidermal barrier. Inhibition of HCV infection by blocking of Cldn1 with cCPE variants was analyzed in the Huh7.5 hepatoma cell line. A model of reconstructed human epidermis was used to investigate modulation of the epidermal barrier by cCPE variants. In contrast to cCPEwt, the Cldn1-binding cCPE-S305P/S307R/S313H inhibited infection of Huh7.5 cells with HCV in a dose-dependent manner. In addition, TJ modulation by cCPE variant-mediated targeting of Cldn1 and Cldn4 opened the epidermal barrier in reconstructed human epidermis. cCPE variants are potent claudin modulators. They can be applied for mechanistic in vitro studies and might also be used as biologics for therapeutic claudin targeting including HCV treatment (host-targeting antivirals) and improvement of drug delivery.
Epigenetics of Urological Cancers
Authors: Schulz Wolfgang A. , Sørensen Karina D.
Published: September 2019

The major urological cancers comprise prostate adenocarcinoma, urinary bladder (or upper urinary tract) carcinoma, renal cell carcinoma, testicular cancer and penile carcinoma, in this order of incidence, each with various histological and molecular subtypes [...]
More Than an Adipokine: The Complex Roles of Chemerin Signaling in Cancer
Authors: Goralski Kerry B. , Jackson Ashley E. , McKeown Brendan T. , Sinal Christopher J.
Published: September 2019

Chemerin is widely recognized as an adipokine, with diverse biological roles in cellular differentiation and metabolism, as well as a leukocyte chemoattractant. Research investigating the role of chemerin in the obesity–cancer relationship has provided evidence both for pro- and anti-cancer effects. The tumor-promoting effects of chemerin primarily involve direct effects on migration, invasion, and metastasis as well as growth and proliferation of cancer cells. Chemerin can also promote tumor growth via the recruitment of tumor-supporting mesenchymal stromal cells and stimulation of angiogenesis pathways in endothelial cells. In contrast, the majority of evidence supports that the tumor-suppressing effects of chemerin are immune-mediated and result in a shift from immunosuppressive to immunogenic cell populations within the tumor microenvironment. Systemic chemerin and chemerin produced within the tumor microenvironment may contribute to these effects via signaling through CMKLR1 (chemerin1), GPR1 (chemerin2), and CCLR2 on target cells. As such, inhibition or activation of chemerin signaling could be beneficial as a therapeutic approach depending on the type of cancer. Additional studies are required to determine if obesity influences cancer initiation or progression through increased adipose tissue production of chemerin and/or altered chemerin processing that leads to changes in chemerin signaling in the tumor microenvironment.
Discovery of 3-Arylquinoxaline Derivatives as Potential Anti-Dengue Virus Agents
Authors: Tseng Chih-Hua , Han Cheng-Ruei , Tang Kai-Wei
Published: September 2019

We designed and synthesized a series of novel 3-arylquinoxaline derivatives and evaluated their biological activities as potential dengue virus (DENV) replication inhibitors. Among them, [3-(4-methoxyphenyl)quinoxalin-2-yl](phenyl)methanol (19a), [6,7-dichloro-3-(4-methoxyphenyl)quinoxalin-2-yl](phenyl)methanol (20a), and (4-methoxyphenyl)(3-phenylquinoxalin-2-yl)methanone (21b) were found to significantly inhibit the DENV RNA expression in Huh-7-DV-Fluc cells with a potency better than that of ribavirin. Compound 19a reduced DENV replication in both viral protein and messenger RNA (mRNA) levels in a dose-dependent manner and exhibited no significant cell cytotoxicity. Notably, compound 19a exhibited a half maximal effective concentration (EC50) value at 1.29 ± 0.74 μM. We further observed that the inhibitory effect of 19a on DENV replication was due to suppression of DENV-induced cyclooxygenase-2 (COX-2) expression. Docking studies also showed that 19a caused hydrophobic interactions at the active sites with Arg29, Glu31, Tyr116, Leu138, Pro139, Lys454, Arg455, and Gln529. The calculated lowest binding energy between the 19a and COX-2 was −9.10 kcal/mol. In conclusion, compound 19a might be a potential lead compound for developing an anti-DENV agent.
Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway
Authors: Jin Shan , Shi Kuan , Liu Liu , Chen Yu , Yang Guangzhong
Published: September 2019

Xanthones are important chemical constituents of Garcinia xanthochymus and varied bioactivities including cytotoxicity. However, their anti-tumor mechanism has remained unknown. Here, we isolated and identified a new xanthone named garciniaxanthone I (1) and five known compounds from the bark of G. xanthochymus. Their structures were elucidated by NMR analysis and HRESIMS. The anti-proliferation activities of all isolated compounds were evaluated on four human tumor cell lines (HepG2, A549, SGC7901, MCF-7). The results demonstrated that the anti-proliferation activity of xanthone was related to the number and location of prenyl groups. We further found that garciniaxanthone I (GXI) could induce HepG2 apoptosis and enhance the expression of cleaved caspase-8, caspase-9, and caspase-3. GXI could also increase Bax level and concurrently reduce the overexpression of Bcl-2, Bcl-XL, Mcl-1, and surviving in HepG2 cells. Moreover, GXI could inhibit cell migration of HepG2 cells by inhibiting the expressions of MMP-7 and MMP-9. In summary, our study suggests that GXI could induce HepG2 apoptosis via the mitochondrial pathway and might become a lead compound for liver cancer treatment.
Plant DNA Polymerases
Authors: Pedroza-Garcia Jose-Antonio , De Veylder Lieven , Raynaud Cécile
Published: September 2019

Maintenance of genome integrity is a key process in all organisms. DNA polymerases (Pols) are central players in this process as they are in charge of the faithful reproduction of the genetic information, as well as of DNA repair. Interestingly, all eukaryotes possess a large repertoire of polymerases. Three protein complexes, DNA Pol α, δ, and ε, are in charge of nuclear DNA replication. These enzymes have the fidelity and processivity required to replicate long DNA sequences, but DNA lesions can block their progression. Consequently, eukaryotic genomes also encode a variable number of specialized polymerases (between five and 16 depending on the organism) that are involved in the replication of damaged DNA, DNA repair, and organellar DNA replication. This diversity of enzymes likely stems from their ability to bypass specific types of lesions. In the past 10–15 years, our knowledge regarding plant DNA polymerases dramatically increased. In this review, we discuss these recent findings and compare acquired knowledge in plants to data obtained in other eukaryotes. We also discuss the emerging links between genome and epigenome replication.
When Personalization Is Not an Option: An In-The-Wild Study on Persuasive News Recommendation
Authors: Gena Cristina , Grillo Pierluigi , Lieto Antonio , Mattutino Claudio , Vernero Fabiana
Published: September 2019

Aiming at granting wide access to their contents, online information providers often choose not to have registered users, and therefore must give up personalization. In this paper, we focus on the case of non-personalized news recommender systems, and explore persuasive techniques that can, nonetheless, be used to enhance recommendation presentation, with the aim of capturing the user’s interest on suggested items leveraging the way news is perceived. We present the results of two evaluations “in the wild”, carried out in the context of a real online magazine and based on data from 16,134 and 20,933 user sessions, respectively, where we empirically assessed the effectiveness of persuasion strategies which exploit logical fallacies and other techniques. Logical fallacies are inferential schemes known since antiquity that, even if formally invalid, appear as plausible and are therefore psychologically persuasive. In particular, our evaluations allowed us to compare three persuasive scenarios based on the Argumentum Ad Populum fallacy, on a modified version of the Argumentum ad Populum fallacy (Group-Ad Populum), and on no fallacy (neutral condition), respectively. Moreover, we studied the effects of the Accent Fallacy (in its visual variant), and of positive vs. negative Framing.
Experimental and Numerical Study of AISI 4130 Steel Surface Hardening by Pulsed Nd:YAG Laser
Authors: Casalino Giuseppe , Moradi Mahmoud , Moghadam Mojtaba Karami , Khorram Ali , Perulli Patrizia
Published: September 2019

Laser surface transformation hardening (LSTH) of AISI 4130 was investigated by a Nd:YAG pulsed laser. Laser focal height (LFH), pulse width (LPW), scanning speed (LSS), and power (LP) varied during the experiments. The microstructure of the treated zone was characterized by optical (OM) and field emission scanning electron microscopy (FESEM). Micro-hardness was measured in the width and depth directions. Results showed that the hardness and depth of hardened layer increased by decreasing the LSS and the laser focal position (LFP), and by increasing the LPW. The results were compared with those obtained by furnace heat treatment of the same steel. Eventually, a finite element model was employed for the simulation of the LSTH of AISI 4130 steel and calculation of the heat-treated zone. The results showed that the model can predict with accuracy the temperature profile and the size and the shape of the laser hardened region.
Predicting the Tensile Behavior of Ti-6.6Al-3.3Mo-1.8Zr-0.29Si Alloy via the Temperature-Dependent Crystal Plasticity Method
Authors: Zhang Jun , Wang Yang , Wang Peng , Chen Junhong , Zheng Songlin
Published: September 2019

Uniaxial tensile flow properties of a duplex Ti-6.6Al-3.3Mo-1.8Zr-0.29Si alloy in a temperature range from 213 K to 573 K are investigated through crystal plasticity modelling. Experimental results indicate that the initial yield stress of the alloy decreases as the temperature increases, while its work-hardening behavior displays temperature insensitivity. Considering such properties of the alloy, the dependence of the initial critical resolved shear stress (CRSS) on temperature is taken into account in the polycrystal plasticity modelling. Good coincidence is obtained between modelling and the experimental results. The determined values of CRSS for slip systems are comparable to the published data. The proposed polycrystalline model provides an alternative method for better understanding the microstructure–property relationship of α + β titanium alloys at different temperatures in the future.
Optimization of Functionally Graded Structural Members by Means of New Effective Properties Estimation Method
Authors: Wiśniewska Anna , Egner Halina
Published: September 2019

An innovative method of effective composite mechanical properties estimation is applied to optimize the distribution of reinforcement in a functionally graded structural element. The concept is based on the assumption of the mechanical equivalence between two configurations: The real heterogeneous composite configuration and the fictitious quasi-homogeneous one. It allows to obtain the analytical formulae describing the dependence of the effective elastic composite properties on the volume fraction of reinforcing inclusions. As an example of application, a circular bar subjected to torsion is considered.
Effectiveness of Reinforcing Bent Non-Uniform Pre-Stressed Glulam Beams with Basalt Fibre Reinforced Polymers Rods
Authors: Wdowiak Agnieszka , Brol Janusz
Published: September 2019

The article presents the testing designating the impact of structural non-uniformity on the effectiveness of reinforcing bent wooden beams reinforced with basalt fibre (BFRP—Basalt Fibre Reinforced Polymers) rods. The obtained results demonstrate a positive impact of the strengthening in improving the bearing capacity and rigidness of the wooden beams. The article presents the impact of selected physical and chemical properties of wooden elements on the achieved strengthening reliability, increase in bearing capacity and the estimation of the reduction of deflections and stresses of bent beams, made from various wood quality classes and reinforced using BFRP rods. The conducted testing featured an analysis of the ability of using lower quality class lumber to strengthen the beams with pre-stressed basalt fibre rods. This solution allows for reducing the cross-section or lower the class of used wood with simultaneous maintenance of comparable rigidity and bending strength of beams, as in the non-strengthened beams.
Synthesis of Poly(3-vinylpyridine)-Block-Polystyrene Diblock Copolymers via Surfactant-Free RAFT Emulsion Polymerization
Authors: Nieswandt Katharina , Georgopanos Prokopios , Abetz Clarissa , Filiz Volkan , Abetz Volker
Published: September 2019

In this work, we present a novel synthetic route to diblock copolymers based on styrene and 3-vinylpyridine monomers. Surfactant-free water-based reversible addition–fragmentation chain transfer (RAFT) emulsion polymerization of styrene in the presence of the macroRAFT agent poly(3-vinylpyridine) (P3VP) is used to synthesize diblock copolymers with molecular weights of around 60 kDa. The proposed mechanism for the poly(3-vinylpyridine)-block-poly(styrene) (P3VP-b-PS) synthesis is the polymerization-induced self-assembly (PISA) which involves the in situ formation of well-defined micellar nanoscale objects consisting of a PS core and a stabilizing P3VP macroRAFT agent corona. The presented approach shows a well-controlled RAFT polymerization, allowing for the synthesis of diblock copolymers with high monomer conversion. The obtained diblock copolymers display microphase-separated structures according to their composition.
Graphene Oxide and Graphene Reinforced PMMA Bone Cements: Evaluation of Thermal Properties and Biocompatibility
Authors: Paz E. , Ballesteros Y. , Abenojar J. , del Real J.C. , Dunne N.J.
Published: September 2019

The incorporation of well-dispersed graphene oxide (GO) and graphene (G) has been demonstrated as a promising solution to improve the mechanical performance of polymethyl methacrylate (PMMA) bone cements in an attempt to enhance the long-term survival of the cemented orthopaedic implants. However, to move forward with the clinical application of graphene-based PMMA bone cements, it is necessary to ensure the incorporation of graphene-based powders do not negatively affect other fundamental properties (e.g., thermal properties and biocompatibility), which may compromise the clinical success of the implant. In this study, the effect of incorporating GO and G on thermal properties, biocompatibility, and antimicrobial activity of PMMA bone cement was investigated. Differential scanning calorimetry studies demonstrated that the extent of the polymerisation reaction, heat generation, thermal conductivity, or glass transition temperature were not significantly (p > 0.05) affected by the addition of the GO or G powders. The cell viability showed no significant difference (p > 0.05) in viability when MC3-T3 cells were exposed to the surface of G- or GO-PMMA bone cements in comparison to the control. In conclusion, this study demonstrated the incorporation of GO or G powder did not significantly influence the thermal properties or biocompatibility of PMMA bone cements, potentially allowing its clinical progression.
Effect of Oxygen Content on Current Stress-Induced Instability in Bottom-Gate Amorphous InGaZnO Thin-Film Transistors
Authors: Choi Sungju , Kim Jae-Young , Kang Hara , Ko Daehyun , Rhee Jihyun , Choi Sung-Jin , Kim Dong Myong , Kim Dae Hwan
Published: September 2019

The effect of oxygen content on current-stress-induced instability was investigated in bottom-gate amorphous InGaZnO (a-IGZO) thin-film transistors. The observed positive threshold voltage shift (ΔVT) was dominated by electron trapping in the gate insulator (GI), whereas it was compensated by donor creation in a-IGZO active regions when both current flows and a high lateral electric field were present. Stress-induced ΔVT increased with increasing oxygen content irrespective of the type of stress because oxygen content influenced GI quality, i.e., higher density of GI electron traps, as well as typical direct current (DC) performance like threshold voltage, mobility, and subthreshold swing. It was also found that self-heating became another important mechanism, especially when the vertical electric field and channel current were the same, independent of the oxygen content. The increased ΔVT with oxygen content under positive gate bias stress, positive gate and drain bias stress, and target current stress was consistently explained by considering a combination of the density of GI electron traps, electric field relaxation, and self-heating-assisted electron trapping.
Equivalent Method of Joint Interface Based on Persson Contact Theory: Virtual Material Method
Authors: Han Renxiu , Li Guoxi , Gong Jingzhong , Zhang Meng , Zhang Kai
Published: September 2019

An accurate equivalent method of metal joint interface is of great significance to optimize the dynamic performance of the whole machine. Therefore, it is necessary to establish an accurate equivalent method of joint interface. The virtual material method is a precise equivalent method of joint interface. The traditional virtual material method is based on the M–B fractal contact theory. By modeling the contact mechanics of the joint interface, the physical properties of the virtual material are obtained separately, such as elastic modulus, Poisson’s ratio and density. In this paper, Persson contact theory is used to establish the interface contact mechanics model to find the physical properties of virtual materials. The virtual material methods constructed by two theories are respectively applied to the modal simulation to obtain the natural frequencies of the joint interface. By comparing the natural frequencies obtained by modal experiment and modal simulation, it is found that the natural frequencies obtained by the virtual material method based on Persson contact theory are closer to the results obtained by the modal experiment, and the error is within 5%. The error of the natural frequencies obtained by the virtual material method based on the M–B fractal contact theory is within 10%. Therefore, the Persson contact theory can establish a more accurate equivalent method of metal’s joint interface.
Laser Micro-Texturing of Sintered Tool Materials Surface
Authors: Pakuła Daniel , Staszuk Marcin , Dziekońska Małgorzata , Kožmín Pavel , Čermák Adam
Published: September 2019

The purpose of this paper is to show the effect of tool materials surface treatment while using laser texturing on the structure and properties of cemented carbides and sialon ceramics. The tests were made on multi-point inserts subjected laser texturization and honeycomb-like texture was obtained. Comprehensive investigations in the scanning electron microscope (SEM) were made. Morphology was examined by the use of atomic forces microscope (AFM) and confocal microscope. The chemical composition of the tested materials using energy-dispersive X-ray spectrometer (EDS) was investigated. Moreover, exploitative properties, including wear resistance using the "pin on disc" method and roughness, were also tested. It was found that the laser texturing provides a suitable modification of the structure improving tribological properties. Tests suggest that laser texturing can contribute to the durability of cutting tool’s edge, which qualifies this type of surface treatment for wide industrial applications.
Solid-State Characterization and Compatibility Studies of Penciclovir, Lysine Hydrochloride, and Pharmaceutical Excipients
Authors: Meira Rafaela Z. C. , Biscaia Isabela F. B. , Nogueira Camila , Murakami Fabio S. , Bernardi Larissa S. , Oliveira Paulo R.
Published: September 2019

The physical and chemical characterization of the solid-state properties of drugs and excipients is fundamental for planning new formulations and developing new strategies for the treatment of diseases. Techniques such as differential scanning calorimetry, thermogravimetry, X-ray powder diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy are among the most commonly used techniques for these purposes. Penciclovir and lysine are individually used to treat the herpes virus. As such, the development of a formulation containing both drugs may have therapeutic potential. Solid-state characterization showed that both penciclovir and lysine were crystalline materials with melting points at 278.27 °C and 260.91 °C, respectively. Compatibility studies of penciclovir and lysine indicated a possible interaction between these substances, as evidenced by a single melting point at 253.10 °C. The compatibility of several excipients, including ethylenediaminetetraacetic acid, cetostearyl alcohol, sodium lauryl sulphate, di-tert-butyl methyl phenol, liquid petrolatum, methylparaben, nonionic wax, paraffin, propylene glycol, and propylparaben, was evaluated in ternary (penciclovir-lysine-excipient) mixtures (1:1:1, w/w/w) to determine the optimal formulation. The developed formulation was stable under accelerated and ambient conditions, which demonstrated that the interaction between penciclovir and lysine was suitable for the development of a formulation containing both drugs.
Thermo-Mechanical Coupling Analyses for Al Alloy Brake Discs with Al2O3-SiC(3D)/Al Alloy Composite Wear-Resisting Surface Layer for High-Speed Trains
Authors: Jiang Lan , Jiang Yanli , Yu Liang , Yang Hongliang , Li Zishen , Ding Youdong
Published: September 2019

In the present work, a theoretical model of three-dimensional (3D) transient temperature field for Al alloy brake discs with Al2O3-SiC(3D)/Al alloy wear-resisting surface layer was established. 3D transient thermo-stress coupling finite element (FE) and computational fluid dynamic (CFD) models of the brake discs was presented. The variation regularities of transient temperature and internal temperature gradient of the brake discs under different emergency braking conditions were obtained. The effects of initial braking velocity (IBV) and thickness of Al2O3-SiC(3D)/Al alloy composite wear-resisting layer on the maximum friction temperature evolution of the disc were discussed. The results indicated the lower temperature and thermal stress distributed uniformly on the wear-resisting surface, which was dominated by high conductivity and cooling ability of the Al alloy brake disc. The maximum friction temperature was not obviously affected by the thickness of the wear-resisting layer. The maximum friction temperature of the brake discs increased with the increase of the IBV, the maximum friction temperature and thermal stress of the brake discs is about 517 °C and 192 MPa at IBV = 97 m/s considering air cooling, respectively. The lower thermal stress and fewer thermal cracks are produced during the braking process, which relatively decrease the damage. The friction behavior of the tribo-couple predicted using FE method correlated well with the experimental results obtained by sub-scale testing.
Incorporation of Additives and Fibers in Porous Asphalt Mixtures: A Review
Authors: Gupta Anik , Rodriguez-Hernandez Jorge , Castro-Fresno Daniel
Published: September 2019

Despite the numerous benefits for preserving the hydrological cycle, permeable pavement systems (PPSs) found their major application in parking spots and for light traffic scenarios due to their limited durability and strength. To make the PPSs suitable for heavy traffic conditions without significant distresses, research is shifting toward the adoption of novel binders and additives for designing multifunctional porous asphalt mixtures which make up the surface course of PPSs. Certain additives are well known for enhancing the durability of dense graded asphalt mixtures and improving fatigue and rutting resistance. However, the studies on the influence of additives on abrasion resistance and binder draindown, which are the common problems in porous asphalt mixtures (PAMs), are still not well established. This paper summarizes best practices performed on PAMs and recommends possible future research directions for its improvement. Particular emphasis is placed on strength and resilience of PAMs by incorporating additives like nanosilica, crumb rubber, warm-mix additives, fibers (such as cellulose, glass, steel, and synthetic fibers), and some eco-friendly materials. It was found that different additives seem to have different effects on the properties of PAMs. Moreover, the combination of additives has synergistic benefits for the performance of PAMs, especially in urban pavements.
Lead-Bismuth Eutectic: Atomic and Micro-Scale Melt Evolution
Authors: Montanari Roberto , Varone Alessandra , Gregoratti Luca , Kaciulis Saulius , Mezzi Alessio
Published: September 2019

Element clustering and structural features of liquid lead-bismuth eutectic (LBE) alloy have been investigated up to 720 °C by means of high temperature X-ray diffraction (HT-XRD), X-ray Photoemission Spectroscopy (XPS) and Scanning Photoemission Microscopy (SPEM) at the Elettra synchrotron in Trieste. The short-range order in liquid metal after melting corresponds to the cuboctahedral atomic arrangement and progressively evolves towards the icosahedral one as temperature increases. Such process, that involve a negative expansion of the alloy, is mainly connected to the reduction of atom distance in Pb–Pb pairs which takes place from 350 °C to 520 °C. On an atomic scale, it is observed a change of the relative number of Bi–Bi, Pb–Pb, and Pb–Bi pairs. The Pb–Bi pairs are detected only at a temperature above ~350 °C, and its fraction progressively increases, giving rise to a more homogeneous distribution of the elements. SPEM results showed evidence that the process of chemical homogenization on an atomic scale is preceded and accompanied by homogenization on micro-scale. Clusters rich of Bi and Pb, which are observed after melting, progressively dissolve as temperature increases: Only a few residuals remain at 350 °C, and no more clusters are detected a 520 °C.
Effects of Ultrasonic Introduced by L-Shaped Ceramic Sonotrodes on Microstructure and Macro-Segregation of 15t AA2219 Aluminum Alloy Ingot
Authors: Zeng Tao , Zhou YaJun
Published: September 2019

The effects of ultrasonic introduced by L-shaped sonotrodes made of high-temperature-resistant ceramic on the microstructure and macro-segregation of solidifying 15t AA2219 aluminum alloy ingots have been examined in the present study. The macroscopic morphology of the corrosion of the sonotrode has been observed. Grain refinement has been observed, the shape and size of the precipitated phase of the ingot were counted, and the degree of segregation along the transverse direction at 500 mm from the head of the ingot has been evaluated. The results reveal that the L-shaped ceramic ultrasonic introduction device can effectively avoid the erosion of high-temperature melt on the sonotrode and the heat radiation of the high-temperature heat flow to the transducer. Furthermore, the scanning electron microscope (SEM) and chemical composition detection results also indicate that the inter-dendritic micro-segregation of the equiaxed grains can be reduced, and the macro-segregation of the chemical composition of the ingot can be suppressed, and more homogeneous microstructures can be obtained when ultrasonic has been applied during solidification.
Thermodynamic Properties of Stoichiometric Non-Superconducting Phase Y2BaCuO5
Authors: Antončík Filip , Sedmidubský David , Jiříčková Adéla , Lojka Michal , Hlásek Tomáš , Růžička Květoslav , Jankovský Ondřej
Published: September 2019

Y2BaCuO5 often occurs as an accompanying phase of the well-known high-temperature superconductor YBa2Cu3O7 (also known as YBCO). Y2BaCuO5, easily identifiable due to its characteristic green coloration, is often referred to as ‘green phase’ or ‘Y-211’. In this contribution, Y2BaCuO5 phase was studied in detail with a focus on its thermal and thermodynamic properties. Energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and scanning electron microscopy (SEM) were employed in the study of sample’s morphology and chemical composition. XRD data were further analyzed and lattice parameters refined by Rietveld analysis. Simultaneous thermal analysis was employed to study thermal stability. Particle size distribution was analyzed by laser diffraction. Finally, thermodynamic properties, namely heat capacity and relative enthalpy, were measured by drop calorimetry, differential scanning calorimetry (DSC), and physical properties measurement system (PPMS). Enthalpy of formation was assessed from ab-initio DFT calculations.
Bending Performance and Reinforcement of Rocker Panel Components with Unidirectional Carbon Fiber Composite
Authors: Yu Huili , Zhao Hui , Shi Fangyuan
Published: September 2019

Unidirectional carbon fiber composite material is one of the most common types of composites employed in vehicles, and its bending performance plays an important role in crash safety, especially in side pole impact. This study aimed to redesign one of the most important components of the side structure of a vehicle, the rocker panel, with unidirectional carbon fiber composite material. Our results show that it is not easy to acquire the same bending performance as that of a steel rocker panel by merely replacing it with carbon fiber material and increasing the wall thickness. Therefore, reinforcements were employed to improve the bending performance of the carbon fiber rocker panel, and a polypropylene reinforcement method achieved a weight reduction of 40.7% compared with high-strength steel.
Biocompatibility of Polyimides: A Mini-Review
Authors: Constantin Catalin P. , Aflori Magdalena , Damian Radu F. , Rusu Radu D.
Published: September 2019

Polyimides (PIs) represent a benchmark for high-performance polymers on the basis of a remarkable collection of valuable traits and accessible production pathways and therefore have incited serious attention from the ever-demanding medical field. Their characteristics make them suitable for service in hostile environments and purification or sterilization by robust methods, as requested by most biomedical applications. Even if PIs are generally regarded as “biocompatible”, proper analysis and understanding of their biocompatibility and safe use in biological systems deeply needed. This mini-review is designed to encompass some of the most robust available research on the biocompatibility of various commercial or noncommercial PIs and to comprehend their potential in the biomedical area. Therefore, it considers (i) the newest concepts in the field, (ii) the chemical, (iii) physical, or (iv) manufacturing elements of PIs that could affect the subsequent biocompatibility, and, last but not least, (v) in vitro and in vivo biocompatibility assessment and (vi) reachable clinical trials involving defined polyimide structures. The main conclusion is that various PIs have the capacity to accommodate in vivo conditions in which they are able to function for a long time and can be judiciously certified as biocompatible.
Neopentyl Glycol as Active Supporting Media in Shape-Stabilized PCMs
Authors: Serrano Angel , Dauvergne Jean-Luc , Doppiu Stefania , Palomo Del Barrio Elena
Published: September 2019

The present work explores the feasibility of using polyalcohols with solid-solid phase transition as active supporting matrix of n-alkanes in shape-stabilized phase change materials (SSPCMs). It is well-established that the use of SSPCM avoids leakage and increases stability and easy handling of solid-liquid PCMs. Nevertheless, the resulting composite exhibits a loss of heat storage capacity due to the volume occupied by the supporting material, which does not contribute to latent heat storage. Therefore, the objective of this work is to combine solid-liquid PCMs (alkanes) with solid-solid PCMs (polyalcohols), both exhibiting a phase transition in the same range of temperature, to obtain high energy density SSPCMs. Towards that goal, the performance of Neopentyl Glycol (NPG) and Docosane as a new energetic SSPCM has been proved. The NPG-Docosane chemical compatibility and its outstanding wettability facilitate the propitious association of both materials. The higher capillary forces obtained by decreasing the NPG crystal size together with the addition of expanded graphite (EG) allowed to obtain a maximum Docosane content of 60 wt%. The addition of EG improves the shape stability at the time that increases the heat transfer properties of the composites. The analysis showed that the components of the obtained SSPCMs are able to combine their latent heats, achieving a maximum value of 210.74 J/g for the highest Docosane content. This value is much higher than those latent heats exhibited by existing SSPCMs in the same working temperature range.
Maturity and Strength Development of Mortar with Antifreezing Admixture at Temperatures Lower than 0 °C
Authors: Choi Hyeonggil , Hama Yukio , Taniguchi Madoka
Published: September 2019

This study investigated the effect of temperature and time at temperatures lower than 0 °C on mortar mixed with antifreezing admixture to determine the temperature–time function with the aim of expressing the effect universally. As a result, the maturity equation for temperatures lower than 0 °C proposed in previous studies was verified to be applicable to type-B blast furnace slag cement. The applicability of this equation at temperatures lower than 0 °C had not been investigated hitherto. The strength development attributable to the effect of the antifreezing admixture can be expressed as the reference temperature, and the reduction in the chemical potential of water chemical potential reduction was found to depend on the reaction rate. A new maturity equation for temperatures lower than 0 °C was proposed considering the effect of the antifreezing admixture.
Coupling Effects of pH and Dissolved Oxygen on the Corrosion Behavior and Mechanism of X80 Steel in Acidic Soil Simulated Solution
Authors: Wang Shuaixing , Yin Xiaole , Zhang Hao , Liu Daoxin , Du Nan
Published: September 2019

In an acidic red soil environment, the corrosion mechanism of X80 steel may be closely related to the pH value and oxygen content, but it has not yet formed a systematic understanding. In this paper, the coupling effects of pH and dissolved oxygen on the corrosion behavior and mechanism of X80 steel in an acidic soil simulated solution were further analyzed by electrochemical methods and three-dimensional video microscope. Results showed that the hydrogen reduction reaction was almost the only cathode process in the anoxic and low pH system, and small and dense pits were present on the electrode surface. pH value increased, the pits decreased, but the size of pits increased. In the oxygen-adequate system, oxygen-consuming (OC) corrosion preferentially occurred, and a protective corrosion product layer (including FeOOH, Fe3O4, etc.) might be formed accordingly, but the proportion of hydrogen evolution (HE) increased and the product layer had defects at a low pH environment. The specific corrosion mechanism of X80 steel in an acidic soil simulated solution is described in the relevant models.
Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device
Authors: Phiphattanaphiphop Chalinee , Leksakul Komgrit , Phatthanakun Rungrueang , Suthummapiwat Apirak
Published: September 2019

In this research, we aimed to count the ratio of the number of motile to immotile sperm for patients with infertility problems based on a low-sperm-concentration examination. The microfluidic system consists of two series of applications: The conventional separation of motile sperm and the proposed inductance (L), capacitance (C), and resistance (R) or LCR impedance sperm counter. In the experiment, 96% of motile sperm were isolated from nonmotile sperm in the first part and transported to the second part to count and calculate real-time sperm concentration. A pair of microelectrodes composed of thin metal film were integrated between microchannels, resulting in a peak signal for LCR single-cell detection, as well as the estimated total sperm concentration. A minimum of 10 µL of the sperm sample was completely analyzed with an accuracy of 94.8% compared with the standard computer-assisted semen analysis (CASA) method. This method could be applied for low-cost sperm separation and counting in the future.
Assessment of Stickiness with Pressure Distribution Sensor Using Offset Magnetic Force
Authors: Kameoka Takayuki , Takahashi Akifumi , Yem Vibol , Kajimoto Hiroyuki , Matsumori Kohei , Saito Naoki , Arakawa Naomi
Published: September 2019

The quantification of stickiness experienced upon touching a sticky or adhesive substance has attracted intense research attention, particularly for application to haptics, virtual reality, and human–computer interactions. Here, we develop and evaluate a device that quantifies the feeling of stickiness experienced upon touching an adhesive substance. Keeping in mind that a typical pressure distribution sensor can only measure a pressing force, but not a tensile force, in our setup, we apply an offset pressure to a pressure distribution sensor and measure the tensile force generated by an adhesive substance as the difference from the offset pressure. We propose a method of using a magnetic force to generate the offset pressure and develop a measuring device using a magnet that attracts magnetic pin arrays and pin magnets; the feasibility of the method is verified with a first prototype. We develop a second prototype that overcomes the noise problems of the first, arising from the misalignment of the pins owing to the bending of the magnetic force lines at the sensor edges. We also obtain measurement results for actual samples and standard viscosity liquids. Our findings indicate the feasibility of our setup as a suitable device for measuring stickiness.
Microfluidic Passive Flow Regulatory Device with an Integrated Check Valve for Enhanced Flow Control
Authors: Zhang Xinjie , Zhang Zhenyu
Published: September 2019

A passive microvalve has appealing advantages in cost-effective and miniaturized microfluidic applications. In this work, we present a passive flow regulatory device for enhanced flow control in a microfluidic environment. The device was integrated with two functional elements, including a flow regulating valve and a flow check valve. Importantly, the flow regulating valve could maintain a stable flow rate over a threshold liquid pressure, and the flow check valve enabled effective liquid on/off control, thus accurate forward flow without any backward leakage was achieved. The flow performance of the flow regulating valve was analyzed through 3D FSI (Fluid-Structure Interaction) simulation, and several key parameters (i.e., fluidic channel height and width, control channel length, and Young’s modulus) were found to influence valve flow rate directly. To examine the flow characteristics of the device, we fabricated a prototype using 3D printing and UV laser cutting technologies, and the flow rates of the prototype under varied test pressures were measured in forward and reverse modes, respectively. Experimental results showed that nearly a constant flow rate of 0.42 ± 0.02 mL s−1 was achieved in the forward mode at an inlet pressure range of 70 kPa to 130 kPa, and liquid flow was totally stopped in the reverse mode at a maximum pressure of 200 kPa. The proposed microfluidic flow regulatory device could be employed for accurate flow control in low-cost and portable Lab-on-a-Chip (LoC) applications.
Ionic Liquid-Nanostructured Poly(Methyl Methacrylate)
Authors: Fedosse Zornio Clarice , Livi Sébastien , Duchet-Rumeau Jannick , Gerard Jean-François
Published: September 2019

Here, ionic liquids (ILs) based on imidazolium and ammonium cations were used as modifying agents for poly(methyl methacrylate) (PMMA) by extrusion. The effects of the chemical nature of the cation and/or counter anion on the resulting properties of IL-modified PMMA blends were analyzed. It was found that the use of low amounts of ILs (2 wt.%) improved the thermal stability. A plasticizing effect of ILs is evidenced by a decrease in glass transition temperature Tg of the modified PMMA, allowing to get large strains at break (i.e., up to 280% or 400%) compared to neat PMMA. The deformation and fracture mechanisms of PMMA under uniaxial tensile stress (i.e., crazing) reveal that the presence of IL delayed the strain during the initiation step of crazing.
Shape Dependent EMA Model of Nanostructured Anisotropic Materials
Authors: Otipka Petr , Vlček Jaroslav
Published: September 2019

Heterogeneous nanostructures containing nanoparticles of various sizes and shapes have attracted significant attention in the development of nano-biosensors. Especially, plasmonic properties of such materials are advantageously exploited for the detection of biological and chemical substances. Since these media exhibit optical anisotropy, a valid homogenization procedure must be able to describe appropriately the relationship between the geometry of the inclusions and the nature of local field modes. We present a model approach for extension of the effective medium approximation (EMA) and its application to anisotropic nanostructures. The proposed model is based on a “strong-couple-dipole” (SCD) method including a volume-integral correction term in a Green tensor that enables to obtain more accurate representation of polarizability tensor. Derived depolarization factors for discs and bi-cone particles are compared with the early known shapes (spheroids, cylinders) and applied to nanostructures composed of the Fe or Au nanodots in polyacrylate.
Ketogenic Diets and Exercise Performance
Authors: Harvey Kristin L. , Holcomb Lola E. , Kolwicz Stephen C.
Published: September 2019

The ketogenic diet (KD) has gained a resurgence in popularity due to its purported reputation for fighting obesity. The KD has also acquired attention as an alternative and/or supplemental method for producing energy in the form of ketone bodies. Recent scientific evidence highlights the KD as a promising strategy to treat obesity, diabetes, and cardiac dysfunction. In addition, studies support ketone body supplements as a potential method to induce ketosis and supply sustainable fuel sources to promote exercise performance. Despite the acceptance in the mainstream media, the KD remains controversial in the medical and scientific communities. Research suggests that the KD or ketone body supplementation may result in unexpected side effects, including altered blood lipid profiles, abnormal glucose homeostasis, increased adiposity, fatigue, and gastrointestinal distress. The purpose of this review article is to provide an overview of ketone body metabolism and a background on the KD and ketone body supplements in the context of obesity and exercise performance. The effectiveness of these dietary or supplementation strategies as a therapy for weight loss or as an ergogenic aid will be discussed. In addition, the recent evidence that indicates ketone body metabolism is a potential target for cardiac dysfunction will be reviewed.
Dietary Fiber, Whole Grains, and Head and Neck Cancer Prognosis: Findings from a Prospective Cohort Study
Authors: Maino Vieytes Christian A. , Mondul Alison M. , Li Zonggui , Zarins Katie R. , Wolf Gregory T. , Rozek Laura S. , Arthur Anna E.
Published: September 2019

No studies, to date, have examined the relationship between dietary fiber and recurrence or survival after head and neck cancer diagnosis. The aim of this study was to determine whether pretreatment intake of dietary fiber or whole grains predicted recurrence and survival outcomes in newly diagnosed head and neck cancer (HNC) patients. This was a prospective cohort study of 463 participants baring a new head and neck cancer diagnosis who were recruited into the study prior to the initiation of any cancer therapy. Baseline (pre-treatment) dietary and clinical data were measured upon entry into the study cohort. Clinical outcomes were ascertained at annual medical reviews. Cox proportional hazard models were fit to examine the relationships between dietary fiber and whole grain intakes with recurrence and survival. There were 112 recurrence events, 121 deaths, and 77 cancer-related deaths during the study period. Pretreatment dietary fiber intake was inversely associated with risk of all-cause mortality (hazard ratio (HR): 0.37, 95% confidence interval (CI): 0.14–0.95, ptrend = 0.04). No statistically significant associations between whole grains and prognostic outcomes were found. We conclude that higher dietary fiber intake, prior to the initiation of treatment, may prolong survival time, in those with a new HNC diagnosis.