bluuebunny/crossref_metadata_2025 huggingface dataset:
The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
Authors: Mound Abdallah , Goormachtigh Gautier , Bray Fabrice , Flament Stéphanie , Rolando Christian , Ruez Richard , Martin Nathalie , Decourcelle Amélie , Dehennaut Vanessa , Saliou Jean-Michel , Chamaillard Mathias , Abbadie Corinne
Published: January 2023

Nod-Like Receptor Pyrin domain-containing protein 6 (NLRP6), a member of the Nucleotide-oligomerization domain-Like Receptor (NLR) family of proteins, assembles together with the ASC protein to form an inflammasome upon stimulation by bacterial lipoteichoic acid and double-stranded DNA. Besides its expression in myeloid cells, NLRP6 is also expressed in intestinal epithelial cells where it may contribute to the maintenance of gut homeostasis and negatively controls colorectal tumorigenesis. Here, we report that NLRP6 is very faintly expressed in several colon cancer cell lines, detected only in cytoplasmic small dots were it colocalizes with ASC. Consequently, it is very hardly detected by standard western-blotting techniques by several presently available commercial antibodies which, in contrast, highly cross-react with a protein of 90kDa that we demonstrate to be unrelated to NLRP6. We report here these results to caution the community not to confuse the 90kDa protein with the endogenous human NLRP6.
A machine learning approach to analyse and predict the electric cars scenario: The Italian case
Authors: Miconi Federico , Dimitri Giovanna Maria
Published: January 2023

The automotive market is experiencing, in recent years, a period of deep transformation. Increasingly stricter rules on pollutant emissions and greater awareness of air quality by consumers are pushing the transport sector towards sustainable mobility. In this historical context, electric cars have been considered the most valid alternative to traditional internal combustion engine cars, thanks to their low polluting potential, with high growth prospects in the coming years. This growth is an important element for companies operating in the electricity sector, since the spread of electric cars is necessarily accompanied by an increasing need of electric charging points, which may impact the electricity distribution network. In this work we proposed a novel application of machine learning methods for the estimation of factors which could impact the distribution of the circulating fleet of electric cars in Italy. We first collected a new dataset from public repository to evaluate the most relevant features impacting the electric cars market. The collected datasets are completely new, and were collected starting from the identification of the main variables that were potentially responsible for the spread of electric cars. Subsequently we distributed a novel designed survey to further investigate such factors on a population sample. Using machine learning models, we could disentangle potentially new interesting information concerning the Italian scenario. We analysed it, in fact, according to different geographical Italian dimensions (national, regional and provincial) and with the final identification of those potential factors that could play a fundamental role in the success and distribution of electric cars mobility. Code and data are available at: https://github.com/GiovannaMariaDimitri/A-machine-learning-approach-to-analyse-and-predict-the-electric-cars-scenario-the-Italian-case.
High fat diet induces obesity, alters eating pattern and disrupts corticosterone circadian rhythms in female ICR mice
Authors: Teeple Kelsey , Rajput Prabha , Gonzalez Maria , Han-Hallett Yu , Fernández-Juricic Esteban , Casey Theresa
Published: January 2023

Circadian, metabolic, and reproductive systems are inter-regulated. Excessive fatness and circadian disruption alter normal physiology and the endocrine milieu, including cortisol, the primary stress hormone. Our aim was to determine the effect feeding a high fat diet to female ICR mice had on diurnal feeding pattern, weight gain, body composition, hair corticosterone levels and circadian patterns of fecal corticosterone. Prepubertal (~35d of age) ICR mice were assigned to control (CON; 10% fat) or high fat (HF; 60% fat) diet and fed for 4 wk to achieve obesity under 12h light and 12h of dark. Feed intake was measured twice daily to determine diurnal intake. Mice were weighed weekly. After 4 wk on diets hair was collected to measure corticosterone, crown-rump length was measured to calculate body mass index (BMI), and body composition was measured with EchoMRI to determine percent fat. HF mice weighed more (P<0.05) after week two, BMI and percent body fat was greater (P<0.05) in HF than CON at the end of wk 4. HF mice consumed more during the day (P<0.05) than CON mice after 1 week on diets. Hair corticosterone was higher in HF mice than in CON (P<0.05). Fecal circadian sampling over 48hr demonstrated that HF mice had elevated basal corticosterone, attenuated circadian rhythms, and a shift in amplitude. High fat feeding for diet induced obesity alters circadian eating pattern and corticosterone rhythms, indicating a need to consider the impact of circadian system disruption on reproductive competence.
Better safe than sorry—Whole-genome sequencing indicates that missense variants are significant in susceptibility to COVID-19
Authors: Słomian Dawid , Szyda Joanna , Dobosz Paula , Stojak Joanna , Michalska-Foryszewska Anna , Sypniewski Mateusz , Liu Jakub , Kotlarz Krzysztof , Suchocki Tomasz , Mroczek Magdalena , Stępień Maria , Sztromwasser Paweł , Król Zbigniew J.
Published: January 2023

Undoubtedly, genetic factors play an important role in susceptibility and resistance to COVID-19. In this study, we conducted the GWAS analysis. Out of 15,489,173 SNPs, we identified 18,191 significant SNPs for severe and 11,799 SNPs for resistant phenotype, showing that a great number of loci were significant in different COVID-19 representations. The majority of variants were synonymous (60.56% for severe, 58.46% for resistant phenotype) or located in introns (55.77% for severe, 59.83% for resistant phenotype). We identified the most significant SNPs for a severe outcome (in AJAP1 intron) and for COVID resistance (in FIG4 intron). We found no missense variants with a potential causal function on resistance to COVID-19; however, two missense variants were determined as significant a severe phenotype (in PM20D1 and LRP4 exons). None of the aforementioned SNPs and missense variants found in this study have been previously associated with COVID-19.
Shifts in age pattern, timing of childbearing and trend in fertility level across six regions of Nigeria: Nigeria Demographic and Health Surveys from 2003–2018
Authors: Olowolafe Tubosun A. , Adebowale Ayo S. , Fagbamigbe Adeniyi F. , Bolarinwa Obasanjo Afolabi , Akinyemi Joshua O.
Published: January 2023

Background

Nigeria’s population is projected to increase from 200 million in 2019 to 450 million in 2050 if the fertility level remains at the current level. Thus, we examined the shifts in the age pattern of fertility, timing of childbearing and trend in fertility levels from 2003 and 2018 across six regions of Nigeria.

Method

This study utilised the 2003, 2008, 2013, and 2018 Nigeria Demographic and Health Survey datasets. Each survey was a cross-sectional population-based design, and a two-stage cluster sampling technique was used to select women aged 15–49 years. The changes in the timing of childbearing were examined by calculating the corresponding mean ages at the birth of different birth orders for each birth order separately to adjust the Quantum effect for births. The Gompertz Relational Model was used to examine the age pattern of fertility and refined fertility level.

Result

In Nigeria, it was observed that there was a minimal decline in mean children ever born (CEB) between 2003 and 2018 across all maternal age groups except aged 20–24 years. The pattern of mean CEB by the age of mothers was the same across the Nigeria regions except in North West. Nigeria’s mean number of CEB to women aged 40–49 in 2003, 2008, 2013 and 2018 surveys was 6.7, 6.6, 6.3 and 6.1, respectively. The mean age (years) at first birth marginally increased from 21.3 in 2003 to 22.5 in 2018. In 2003, the mean age at first birth was highest in South East (24.3) and lowest in North East (19.4); while South West had the highest (24.4) and both North East and North West had the lowest (20.2) in 2018. Similar age patterns of fertility existed between 2003 and 2018 across the regions. Nigeria’s estimated total fertility level for 2003, 2008, 2013 and 2018 was 6.1, 6.1, 5.9 and 5.7, respectively.

Conclusion

The findings showed a reducing but slow fertility declines in Nigeria. The decline varied substantially across the regions. For a downward change in the level of fertility, policies that will constrict the spread of fertility distribution across the region in Nigeria must urgently be put in place.
Impact of sequential herbicides application on crop productivity, weed and nutrient dynamics in soybean under conservation agriculture in Vertisols of Central India
Authors: Vishwakarma A. K. , Meena Bharat Prakash , Das Hiranmoy , Jha Pramod , Biswas A. K. , Bharati K. , Hati K. M. , Chaudhary R. S. , Shirale A. O. , Lakaria B. L. , Gurav Priya P. , Patra Ashok K.
Published: January 2023

Adoption of conservation agriculture (CA) is very slow due to weed infestations. The application of herbicides is the only viable option to deal with problem of weed management to adhere with basic principles of CA. A field experiment was carried out for three years to evaluate the expediency of different herbicides and their sequential applications under CA. In this study, seven treatments comprised of either alone or sequential application of pre-emergence (PE) and post-emergence (PoE) herbicides, hand weeding and weedy check were tested in soybean. Result indicated that sequential application of glyphosate at 1 kg ai ha-1+ pendimethalin at 1 kg ai ha-1as PE followed by PoE application of imazethapyr at 100 g ai ha-1at 30 days after sowing (DAS) proved to be the best economical option in terms of plant growth parameters, crop biomass, seed yield, weed index and carbon and nutrient recycling. Pearson’s correlation coefficients matrix revealed that grain yield was significantly (P<0.0001) related to weed density at harvest (r = -0.84), (WDH) (r = -0.63), weed dry biomass (WDB) (r = -0.52), weed nitrogen (N), phosphorus (P) and potassium (K) uptake (r = -0.56, r = -0.59 and r = -0.66), respectively and weed index (WI) (r = -0.96). The bivariate linear regression study of grain yield on weed control efficiency (WCI) biomass, N, P and K uptake by grain showed a clear significant (P<0.0001) dependence with R2value of 0.53, 0.99, 0.95 and 0.98, respectively. The fitted stepwise multiple regression model also revealed that N and P uptake in grain, weed density at 20 DAS and K uptake in weed were actual predictor for grain yield. We concluded that, effective and economical weed control under CA in soybean can be achieved through sequential application of glyphosate along with pendimethalin at 1 kg ai ha-1each PE followed by PoE use of imazethapyr at 100 g ai ha-1at 30 DAS.
Investigating the direct and indirect effects of a school-based leadership program for primary school students: Rationale and study protocol for the ‘Learning to Lead’ cluster randomised controlled trial
Authors: Wade Levi , Beauchamp Mark R. , Nathan Nicole , Smith Jordan J. , Leahy Angus A. , Kennedy Sarah G. , Boyer James , Bao Ran , Diallo Thierno M. O. , Vidal-Conti Josep , Lubans David R.
Published: January 2023

Background

Leadership is a valuable skill that can be taught in school, and which may have benefits within and beyond the classroom. Learning to Lead (L2L) is a student-led, primary school-based leadership program whereby older ‘peer leaders’ deliver a fundamental movement skills (FMS) program to younger ‘peers’ within their own school.

Aim

The aims of the study are to determine the efficacy of a peer-led FMS intervention on: (i) peer leaders’ (aged 10 to 12 years) leadership effectiveness (primary outcome), leadership self-efficacy, well-being, and time on-task in the classroom; (ii) peers’ (aged 8 to 10 years) physical activity levels, actual and perceived FMS competency, cardiorespiratory fitness, muscular power, and executive functioning; and (iii) teachers’ (referred to as ‘school champions’) work-related stress and well-being.

Method

L2L will be evaluated using a two-arm parallel group cluster randomised controlled trial. Twenty schools located within a two-hour drive of the University of Newcastle, Australia will be recruited. We will recruit 80 students (40 peer leaders and 40 peers) from each school (N = 1,600). L2L will be implemented in three phases: Phase 1 –school champions’ training via a professional learning workshop; Phase 2 –school champions’ delivery of leadership lessons to the peer leaders; and Phase 3 –peer leaders’ delivery of the FMS program to their younger peers. The FMS program, consisting of 12 x 30-minute lessons, will be delivered over the course of one school term (10 weeks). Study outcomes will be assessed at baseline (between mid-March to June, Terms 1 and 2), intervention end (mid-August to September, Term 3), and follow-up (November to mid-December, Term 4. This trial was prospectively registered on the Australian New Zealand Clinical Trials Registry (ANZCTR); registration number: ACTRN12621000376842.
The impact of racially-targeted food marketing and attentional biases on consumption in Black adolescent females with and without obesity: Pilot data from the Black Adolescent & Entertainment (BAE) study
Authors: Cassidy Omni , Tanofsky-Kraff Marian , Waters Andrew J. , Shank Lisa M. , Pine Abigail , Quattlebaum Mary , DeLeon Patrick H. , Bragg Marie , Sbrocco Tracy
Published: January 2023

Unhealthy food advertisements (“advertisements” hereafter referred to as “ads”) are linked to poor diet and obesity, and food companies disproportionally target Black youth. Little is known about the mechanisms whereby food ads influence diet. One possibility may be racially-targeted ads that appeal to Black youth. Those with food-related attentional biases may be especially vulnerable. The objective of this project was to assess the feasibility and initial effects of a pilot study testing the influence of racially-targeted food ads and food-related attentional biases on eating behaviors among a sample of Black adolescent females. Feasibility of recruitment, retention, and procedures were examined. Participants (N = 41, 12-17y) were randomized to view a television episode clip of the Big Bang Theory embedded with either four 30-second racially-targeted food ads or neutral ads. A computer dot probe task assessed food-related attentional biases. The primary outcome was caloric consumption from a laboratory test meal. Interactions based on weight and ethnic identity were also examined. Analyses of variance and regressions were used to assess main and interaction effects. Exposure to racially-targeted food ads (versus neutral ads) did not affect energy consumption (p > .99). Although not statistically significant, adolescents with obesity consumed nearly 240 kcal more than non-overweight adolescents (p = 0.10). There were no significant preliminary effects related to food-related attentional biases or ethnic identity (ps = 0.22–0.79). Despite a non-significant interaction, these data provide preliminary support that adolescents with obesity may be particularly vulnerable to racially-targeted food ads. An adequately powered trial is necessary to further elucidate the associations among racially-targeted food ads among Black adolescent girls with obesity.
Phylogenomics of trans-Andean tetras of the genus Hyphessobrycon Durbin 1908 (Stethaprioninae: Characidae) and colonization patterns of Middle America
Authors: Elías Diego J. , McMahan Caleb D. , Alda Fernando , García-Alzate Carlos , Hart Pamela B. , Chakrabarty Prosanta
Published: January 2023

Hyphessobrycon is one of the most species rich and widely distributed genera in the family Characidae, with more than 160 species ranging from Veracruz, Mexico to Mar Chiquita Lagoon in Buenos Aires, Argentina. The majority of Hyphessobrycon diversity shows a cis-Andean distribution; only nine species are trans-Andean including H. compressus (Meek 1908). It is well established that Hyphessobrycon is not monophyletic but it has been suggested that natural groups can be identified within the larger Hyphessobrycon species group. In this study, we tested the monophyly of trans-Andean species of Hyphessobrycon and investigated the placement of H. compressus. We inferred the first phylogenomic hypothesis of trans-Andean Hyphessobrycon that includes nearly complete taxonomic sampling (eight of nine valid species) using ultraconserved elements (UCEs). We analyzed 75% (1682 UCEs), 90% (1258 UCEs), and 95% (838 UCEs) complete data matrices, and inferred phylogenomic hypotheses under concatenation and coalescent approaches. In all cases, we recovered the monophyly of trans-Andean Hyphessobrycon inclusive of H. compressus, strong support for three species groups, and evidence of cryptic diversity within the widespread H. compressus and H. condotensis. We used our phylogenomic hypothesis to investigate the biogeographic history of Hyphessobrycon in Middle America. Our ancestral range estimation analysis suggests a single event of cis- to trans-Andean colonization followed by stepwise colonization from the Pacific slope of northwestern South America (Chocó block) to northern Middle America (Maya block). Our work supports the recognition of the trans-Andean species as Hyphessobrycon sensu stricto and provides an evolutionary template to examine morphological characters that will allow us to better understand the diversity of Hyphessobrycon in Middle America.
A security testing mechanism for detecting attacks in distributed software applications using blockchain
Authors: Algarni Abdullah , Attaallah Abdulaziz , Eassa Fathi , Khemakhem Maher , Jambi Kamal , Aljihani Hosam , Almarhabi Khalid , Albalwy Faisal
Published: January 2023

Distributed software applications are one of the most important applications currently used. Rising demand has led to a rapid increase in the number and complexity of distributed software applications. Such applications are also more vulnerable to different types of attacks due to their distributed nature. Detecting and addressing attacks is an open issue concerning distributed software applications. This paper proposes a new mechanism that uses blockchain technology to devise a security testing mechanism to detect attacks on distributed software applications. The proposed mechanism can detect several categories of attacks, such as denial-of-service attacks, malware and others. The process starts by creating a static blockchain (Blockchain Level 1) that stores the software application sequence obtained using software testing techniques. This sequence information exposes weaknesses in the application code. When the application is executed, a dynamic blockchain (Blockchain Level 2) helps create a static blockchain for recording the responses expected from the application. Every response should be validated using the proposed consensus mechanism associated with static and dynamic blockchains. Valid responses indicate the absence of attacks, while invalid responses denote attacks.
The added value of family-centered rounds in the hospital setting: A systematic review of systematic reviews
Authors: Woldring Josien M. , Luttik Marie Louise , Paans Wolter , Gans Reinold O. B.
Published: January 2023

Background

Family engagement in care for adult inpatients may improve shared decision making in the hospital and the competence and preparedness of informal caregivers to take over the care at home. An important strategy to involve family members in hospital care processes is to include them in (ward) rounds, also called ‘family-centered rounds’(FCRs).

Objectives

Summarize the evidence regarding the added value of FCRs from the perspectives of patients, family, and healthcare professionals.

Methods

A review protocol was registered a priori with PROSPERO (number CRD42022320915). The electronic databases PubMed, CINAHL, and PsycInfo were searched for English-written systematic reviews with a focus on FCRs. The results and methods were presented in line with the PRISMA guidelines, and the methodological quality of the included reviews was assessed using the adapted version of the AMSTAR tool.

Results

Of the 207 initial records, four systematic reviews were identified covering a total of 67 single studies, mainly performed in critical and pediatric care. Added values of FCR were described at review level, with references to single studies. All four systematic reviews reported an improvement in satisfaction among patients, family, and healthcare professionals, whereby satisfaction is linked to improved communication and interaction, improved situational understanding, inclusion of family in the decision-making process, and improved relationships within the care situation.

Conclusion

Although only limited research has been conducted on the value of FCRs in the adult non-critical care setting, and despite the existence of a variety of outcome measures, the results available from the pediatric and acute care setting are positive. The findings of the sole study in an adult non-critical patient population are in line with these results. Further research in adult non-critical care is required to verify its effects in this setting.
Evaluating the cost-effectiveness of testing pregnant women for penicillin allergy
Authors: Thao Viengneesee , Sharpe Emily E. , Dholakia Ruchita , Ahn Hannah H. , Moriarty James P. , Borah Bijan J. , Gill Margaret C. , Theiler Regan N.
Published: January 2023

Introduction

True penicillin allergy is rare and is commonly incorrectly reported. In fact, less than five percent of patients who report a penicillin allergy will have a currently active clinically-significant IgE- or T-cell-mediated hypersensitivity when appropriately tested. Penicillin is the agent of choice for intrapartum antibiotic prophylaxis to reduce the risk of group B streptococcus early-onset disease in the newborn. Inaccurate penicillin allergy status may lead to inappropriate antibiotic use, as most alternative drugs are more expensive and broader spectrum than penicillin. Penicillin allergy testing has been found to be safe in pregnancy and cost-effective in other patient populations.

Objective

To evaluate the cost-effectiveness of penicillin allergy testing and appropriate antibiotic treatment (test then treat strategy) compared to usual care among pregnant women.

Methods

We developed a decision tree to evaluate the cost of providing appropriate care via a test then treat strategy for pregnant women who report a penicillin allergy, compared to usual care.

Results

Using the test then treat strategy the additional cost to ensure appropriate care for all pregnant women who report a penicillin allergy, was $1122.38 per person. Adopting a test then treat strategy increased the number of appropriate antibiotic use from 7,843/10,000 to 10,000/10,000 simulations.

Conclusion

Our results show that a test then treat strategy for pregnant women who report a penicillin allergy is a good-value intervention.
Understanding others’ distal goals from proximal communicative actions
Authors: Dockendorff Martin , Schmitz Laura , Vesper Cordula , Knoblich Günther
Published: January 2023

Many social interactions require individuals to coordinate their actions and to inform each other about their goals. Often these goals concern an immediate (i.e., proximal) action, as when people give each other a brief handshake, but they sometimes also refer to a future (i.e. distal) action, as when football players perform a passing sequence. The present study investigates whether observers can derive information about such distal goals by relying on kinematic modulations of an actor’s instrumental actions. In Experiment 1 participants were presented with animations of a box being moved at different velocities towards an apparent endpoint. The distal goal, however, was for the object to be moved past this endpoint, to one of two occluded target locations. Participants then selected the location which they considered the likely distal goal of the action. As predicted, participants were able to detect differences in movement velocity and, based on these differences, systematically mapped the movements to the two distal goal locations. Adding a distal goal led to more variation in the way participants mapped the observed movements onto different target locations. The results of Experiments 2 and 3 indicated that this cannot be explained by difficulties in perceptual discrimination. Rather, the increased variability likely reflects differences in interpreting the underlying connection between proximal communicative actions and distal goals. The present findings extend previous research on sensorimotor communication by demonstrating that communicative action modulations are not restricted to predicting proximal goals but can also be used to infer more distal goals.
Debriefing works: Successful retraction of misinformation following a fake news study
Authors: Greene Ciara M. , Murphy Gillian
Published: January 2023

In recent years there has been an explosion of research on misinformation, often involving experiments where participants are presented with fake news stories and subsequently debriefed. In order to avoid potential harm to participants or society, it is imperative that we establish whether debriefing procedures remove any lasting influence of misinformation. In the current study, we followed up with 1547 participants one week after they had been exposed to fake news stories about COVID-19 and then provided with a detailed debriefing. False memories and beliefs for previously-seen fake stories declined from the original study, suggesting that the debrief was effective. Moreover, the debriefing resulted in reduced false memories and beliefs for novel fake stories, suggesting a broader impact on participants’ willingness to accept misinformation. Small effects of misinformation on planned health behaviours observed in the original study were also eliminated at follow-up. Our findings suggest that when a careful and thorough debriefing procedure is followed, researchers can safely and ethically conduct misinformation research on sensitive topics.
Effect of acetazolamide on visuomotor performance at high altitude in healthy people 40 years of age or older—RCT
Authors: Reiser Aurelia E. , Furian Michael , Lichtblau Mona , Buergin Aline , Schneider Simon R. , Appenzeller Paula , Mayer Laura , Muralt Lara , Mademilov Maamed , Abdyraeva Ainura , Aidaralieva Shoira , Muratbekova Aibermet , Akylbekov Azamat , Sheraliev Ulan , Shabykeeva Saltanat , Sooronbaev Talant M. , Ulrich Silvia , Bloch Konrad E.
Published: January 2023

Objective

Altitude travel is increasingly popular also for middle-aged and older tourists and professionals. Due to the sensitivity of the central nervous system to hypoxia, altitude exposure may impair visuomotor performance although this has not been extensively studied. Therefore, we investigated whether a sojourn at moderately high altitude is associated with visuomotor performance impairments in healthy adults, 40y of age or older, and whether this adverse altitude-effect can be prevented by acetazolamide, a drug used to prevent acute mountain sickness.

Methods

In this randomized placebo-controlled parallel-design trial, 59 healthy lowlanders, aged 40-75y, were assigned to acetazolamide (375 mg/day, n = 34) or placebo (n = 25), administered one day before ascent and while staying at high altitude (3100m). Visuomotor performance was assessed at 760m and 3100m after arrival and in the next morning (post-sleep) by a computer-assisted test (Motor-Task-Manager). It quantified deviation of a participant-controlled cursor affected by rotation during target tracking. Primary outcome was the directional error during post-sleep recall of adaptation to rotation estimated by multilevel linear regression modeling. Additionally, adaptation, immediate recall, and correct test execution were evaluated.

Results

Compared to 760m, assessments at 3100m with placebo revealed a mean (95%CI) increase in directional error during adaptation and immediate recall by 1.9° (0.2 to 3.5, p = 0.024) and 1.1° (0.4 to 1.8, p = 0.002), respectively. Post-sleep recall remained unchanged (p = NS), however post-sleep correct test execution was 14% less likely (9 to 19, p<0.001). Acetazolamide improved directional error during post-sleep recall by 5.6° (2.6 to 8.6, p<0.001) and post-sleep probability of correct test execution by 36% (30 to 42, p<0.001) compared to placebo.

Conclusion

In healthy individuals, 40y of age or older, altitude exposure impaired adaptation to and immediate recall and correct execution of a visuomotor task. Preventive acetazolamide treatment improved visuomotor performance after one night at altitude and increased the probability of correct test execution compared to placebo.

ClinicalTrials.gov identifier

ClinicalTrials.gov NCT03536520.
Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
Authors: Su Zhenwei , Brooks Jonathan , Pelker Jeffrey , Andreyeva Tatyana , Sobon Hanna , Gifford Roger , Powers Matthew , Wang Jing , Dower Corey , Hegen Martin , Messing Dean , Sheehan Alfredo Darmanin , Brennan Joseph J.
Published: January 2023

C-C motif chemokine receptor-like 2 (CCRL2) is a non-signaling 7 transmembrane receptor that binds chemotactic ligands to shape leukocyte recruitment to sites of inflammation. However, there is a lack of consensus on the ligands that directly bind CCRL2 or their functional impact. Studies with CCRL2 knockout mice have demonstrated that neutrophils have impaired degranulation and migration in response to CXCL8, where the underlying molecular mechanism is proposed to be due to the formation of CCRL2 heterodimers with the chemokine receptor CXCR2. Herein, we characterized the ligands that bind directly to CCRL2 and interrogated the impact of CCRL2 neutralization on CXCL8 signaling in neutrophils using pharmacological antibody tools. Using flow cytometry and Surface Plasmon Resonance microscopy (SPRm) cell binding experiments, we confirmed that chemerin, but not previously reported C-C chemokines, binds CCRL2. Furthermore, we identified human and mouse CCRL2 antibodies that neutralized chemerin binding to CCRL2. Unexpectedly, we found that neutralization of CCRL2 with these antibodies did not attenuate CXCL8-induced human neutrophil degranulation nor CXCL8-induced murine neutrophil recruitment to the peritoneum. Based on the observed differences in modulating CCRL2 function with neutralizing antibodies compared to the reported CCRL2 deficient murine models, we hypothesize that the ligand binding function of CCRL2 is dispensable for CXCL8 signaling in neutrophils. Finally, extensive profiling of CCRL2 expression on peripheral blood leukocytes revealed monocytes, dendritic cells (DC), and subpopulations of natural killer T (NKT) cells as additional targets, highlighting potential roles for CCRL2 in human cell types beyond neutrophils that warrants future investigation.
Midwives’ survey of their weight management practice before and after the GLOWING guideline implementation intervention: A pilot cluster randomised controlled trial
Authors: Heslehurst Nicola , McParlin Catherine , Sniehotta Falko F. , Rankin Judith , McColl Elaine
Published: January 2023

Background

Maternal weight management is a priority due to pregnancy risks for women and babies. Interventions significantly improve maternal diet, physical activity, weight, and pregnancy outcomes. There are complex barriers to midwives’ implementation of guidelines; low self-efficacy is a core implementation barrier. The GLOWING intervention uses social cognitive theory (SCT) to address evidence-based barriers to practice. The intervention aimed to support midwives’ implementation of guidelines.

Methods

An external rehearsal pilot cluster randomised controlled trial in four NHS Trusts (clusters) in England, UK. Clusters were randomised to intervention (where all eligible midwives received the intervention) or control (no intervention delivered) arms. A random sample of 100 midwives were invited to complete questionnaires pre- and post-intervention. UK guideline recommendations relating to midwives’ practice were categorised into: 1) communication-related behaviours (including weight- and risk-communication), and 2) support/intervention-related behaviours (including diet/nutrition, physical activity, weight management, referrals/signposting). Questionnaires were developed using SCT constructs (self-efficacy, outcome expectancies, intentions, behaviours) and 7-point Likert scale, converted to a 0–100 scale. Higher scores were more positive. Descriptive statistics compared intervention and control arms, pre- and post-intervention.

Results

Seventy-four midwives consented and 68 returned questionnaires. Pre-intervention, self-efficacy for support/intervention-related behaviours scored lowest. In controls, there was limited difference between the pre- and post-intervention scores. Post-intervention, mean (SD) scores were consistently higher among intervention midwives than controls, particularly for support/intervention self-efficacy (71.4 (17.1) vs. 58.4 (20.1)). Mean (SD) self-efficacy was higher post-intervention than pre-intervention for all outcomes among intervention midwives, and consistently higher than controls. Mean differences pre- and post-intervention were greatest for support/intervention self-efficacy (17.92, 95% CI 7.78–28.07) and intentions (12.68, 95% CI 2.76–22.59). Self-efficacy was particularly increased for diet/nutrition and physical activity (MD 24.77, 95% CI 14.09–35.44) and weight management (18.88, 95% CI 7.88–29.88) behaviours, which showed the largest increase in scores.

Conclusions

This study supports the theoretical models used to develop GLOWING, where low self-efficacy was a core implementation barrier. Results suggest that GLOWING successfully targets self-efficacy, potentially with a positive impact on guideline implementation. A definitive trial is required to determine effectiveness.

Trial registration

ISRCTN46869894, retrospectively registered 25/05/2016, http://isrctn.com/ISRCTN46869894.
Benefit sharing in international rivers: A Q-methodology study of regional understanding and perception in Asia
Authors: Xie Lei , Xu Lu , Yu Qi
Published: January 2023

This study aims to identify and examine the different perception of benefit sharing in the sharing of international rivers in China, South Asia and Southeast Asia. Using the Q-Method, this study undertakes an in-depth analysis of the views of 35 experts of the field on hydrodiplomacy, international water law, benefit sharing and ecological benefits compensation. The results of the quantitative and qualitative analysis help to innovatively identify three streams of views among the participants, respectively described as supporters, idealists and pragmatists, each displaying strong geographical association to the three Asian regions. Supporters from Southeast Asia and pragmatists from China share much common ground on issues such as the types of benefits to share in international rivers, potentially providing the conceptual foundation for international cooperation. Idealists from South Asia prioritize and emphasise the role and importance of environmental benefits and ecological protection, yet differ greatly from the others on practical issues such as the inclusion of direct economic benefits and ecological compensation for using resources. This study contributes to the understanding of the theory and practice of benefit sharing in international rivers, as well as providing new perspectives to the interpretation and practice of hydrodiplomacy in Asian regions.
Building an annotated corpus for automatic metadata extraction from multilingual journal article references
Authors: Choi Wonjun , Yoon Hwa-Mook , Hyun Mi-Hwan , Lee Hye-Jin , Seol Jae-Wook , Lee Kangsan Dajeong , Yoon Young Joon , Kong Hyesoo
Published: January 2023

Bibliographic references containing citation information of academic literature play an important role as a medium connecting earlier and recent studies. As references contain machine-readable metadata such as author name, title, or publication year, they have been widely used in the field of citation information services including search services for scholarly information and research trend analysis. Many institutions around the world manually extract and continuously accumulate reference metadata to provide various scholarly services. However, manually collection of reference metadata every year continues to be a burden because of the associated cost and time consumption. With the accumulation of a large volume of academic literature, several tools, including GROBID and CERMINE, that automatically extract reference metadata have been released. However, these tools have some limitations. For example, they are only applicable to references written in English, the types of extractable metadata are limited for each tool, and the performance of the tools is insufficient to replace the manual extraction of reference metadata. Therefore, in this study, we focused on constructing a high-quality corpus to automatically extract metadata from multilingual journal article references. Using our constructed corpus, we trained and evaluated a BERT-based transfer-learning model. Furthermore, we compared the performance of the BERT-based model with that of the existing model, GROBID. Currently, our corpus contains 3,815,987 multilingual references, mainly in English and Korean, with labels for 13 different metadata types. According to our experiment, the BERT-based model trained using our corpus showed excellent performance in extracting metadata not only from journal references written in English but also in other languages, particularly Korean. This corpus is available at http://doi.org/10.23057/47.
Alcohol as a risk factor for hearing loss: A systematic review and meta-analysis
Authors: Qian Peiyi , Zhao Zhixin , Liu Shuangyan , Xin Jiarui , Liu Yun , Hao Yinzhu , Wang Yaxin , Yang Lei
Published: January 2023

Objective

Growing evidence suggests that alcohol consumption is a risk factor for hearing loss; however, the evidence has been inconsistent. This systematic review and meta-analysis aimed to evaluate the effect of alcohol consumption on hearing loss.

Methods

We searched several databases up to November 2021, for published articles using the keywords “alcohol drinking” and “hearing loss”. Two investigators independently conducted the study selection and data extraction. Based on the results of the heterogeneity analysis (Q statistic and I2 statistic), a fixed- or random-effects model was used to calculate the pooled odds ratios (ORs). Subgroup and sensitivity analyses were performed to assess the potential sources of heterogeneity and robustness of the pooled estimation. Publication bias in the literature was evaluated using Egger’s test.

Results

In total, 18 (9 cross-sectional, 5 case-control, and 4 cohort) observational studies were identified in this search; 27,849 participants were included. Compared with non-drinkers, the pooled OR of drinkers was 1.22 (95% confidence interval: 1.09–1.35).

Conclusion

Evidence suggests a positive association between alcohol consumption and hearing loss. Drinkers were at a higher risk than non-drinkers. Drinking limitations may be useful for preventing hearing loss.
Implementation research protocol on the national community health policy in Guinea: A sequential mixed-methods study using a decision space approach
Authors: Delamou Alexandre , Grovogui Fassou Mathias , Miller Lior , Nye Amy , Kourouma Mamadi , Kolié Delphin , Goumou Tohanizé , Bossert Thomas J.
Published: January 2023

The overall goal of this study is to explore the rollout of the community health policy in Guinea in the context of decentralization, and the role of decision space (the decision authority, capacities, and accountability of local officials) in explaining gaps between the policy’s conceptualization and actual implementation. The implementation research study will employ a sequential explanatory mixed-methods design. The study will be conducted in 27 communes purposefully selected across the country and include communes where the national community health policy is fully, partially, and not yet being implemented. The quantitative component, based on a survey questionnaire and secondary data, will use ordinary least squares (OLS) multiple regression to compare maternal and child health (MCH) coverage indicators according to the level of policy implementation in the commune. An interrupted time series analysis will be conducted to assess changes in routine MCH service delivery indicators associated with implementation of the community health policy, comparing indicators from one year prior to implementation. OLS regression will be conducted to assess the association between decision space and MCH indicators; all analyses will be carried out in Stata. Findings from the quantitative study will be used to inform the key qualitative questions and areas to explore in greater depth, to develop the interview and focus group guides, and to generate an initial codebook. Qualitative data will be double coded in NVivo by two qualitative analysts, and results generated using thematic analysis. Findings from the quantitative and qualitative components will be integrated and triangulated for interpretation and reporting. Findings and recommendations of this study will inform revisions to the National Community Health Policy to improve its rollout and effectiveness.
Drosophila RhoGAP18B regulates actin cytoskeleton during border cell migration
Authors: Lei Fengyun , Xu Xiaoqing , Huang Jianhua , Su Dan , Wan Ping
Published: January 2023

Drosophila RhoGAP18B was identified as a negative regulator of small GTPase in the behavioral response to ethanol. However, the effect of RhoGAP18B on cell migration is unknown. Here, we report that RhoGAP18B regulates the migration of border cells in Drosophila ovary. The RhoGAP18B gene produces four transcripts and encodes three translation isoforms. We use different RNAi lines to knockdown each RhoGAP18B isoform, and find that knockdown of RhoGAP18B-PA, but not PC or PD isoform, blocks border cell migration. Knockdown of RhoGAP18B-PA disrupts the asymmetric distribution of F-actin in border cell cluster and increases F-actin level. Furthermore, RhoGAP18B-PA may act on Rac to regulate F-actin organization. Our data indicate that RhoGAP18B shows isoform-specific regulation of border cell migration.
Temporal trends in, and associations of, early-career general practitioner prescriptions of second-line Type 2 Diabetes medications, 2010–2018
Authors: Patsan Irena , Tapley Amanda , Davoren Peter , Fielding Alison , Holliday Elizabeth , Ball Jean , Davey Andrew , van Driel Mieke , Turner Rachel , Mulquiney Katie , Spike Neil , FitzGerald Kristen , Magin Parker
Published: January 2023

Introduction

Second-line pharmacotherapy for Type 2 Diabetes Mellitus (‘diabetes’) is necessary for optimal glycaemic control and preventing longer-term complications. We aimed to describe temporal trends in, and associations of, Australian general practitioner (GP) registrars’ prescription, and initiation, of ‘new’ second-line oral agents (dipeptidyl peptidase 4 inhibitors, sodium-glucose cotransporter 2 inhibitors, glucagon-like peptide 1 agonists) compared to sulphonylureas.

Materials and methods

A longitudinal analysis (2010–2018) of data from the Registrar Clinical Encounters in Training project. Analysis included any diabetes problem/diagnosis that involved prescription of sulphonylureas or ‘new’ oral agents. Simple and multiple logistic regression models were fitted within the generalised estimating equations framework.

Results

2333 registrars recorded 6064 diabetes problems/diagnoses (1.4%). 835 problems/diagnoses involved sulphonylurea or ‘new’ medication prescription. Of these, 61.0% [95% CI:57.4–64.4] involved ‘new’ medication prescription. 230 problems/diagnoses involved sulphonylurea or ‘new’ medication initiation, with 77% [95%CI:70.8–82.1] involving a ‘new’ medication. There was a significant 52% per year increase in prescribing (OR = 1.52[95% CI:1.38–1.68],p<0.001), and a 77% per (two-to-three-year) time-interval increase in initiation (OR = 1.77,[95% CI:1.30–2.43],p = <0.001) of ‘new’ medications compared to sulphonylureas. ‘New’ medications were prescribed less for non-English-speaking patients. There was some regional variation in prescribing.

Conclusion

Registrar uptake of ‘new’ oral agents compared to sulphonylureas has increased rapidly.
Prevalence and factors associated with dyslipidemia in Bangladeshi adults
Authors: Ali Nurshad , Samadder Mitu , Kathak Rahanuma Raihanu , Islam Farjana
Published: January 2023

Background

Dyslipidemia is one of the modifiable risk factors for cardiovascular disease and a leading cause of morbidity and mortality worldwide. This study was designed to estimate the prevalence and factors associated with dyslipidemia in Bangladeshi adults.

Methods

A total of 603 participants aged ≥ 18 years were recruited in the study. Serum levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were analyzed using enzymatic colorimetric methods. Dyslipidemia was defined based on serum lipids levels following the standard guidelines by National Cholesterol Education Program Adult Treatment Panel III. Multivariate logistic regression analysis was applied to evaluate risk factors associated with dyslipidemia.

Results

The overall prevalence of dyslipidemia was 89% with no significant difference between male (90.1%) and female (85.7) subjects. The prevalence of hypertriglyceridemia was 51.7%, hypercholesterolemia 41.6%, high LDL 43.9% and low HDL 78.8%. When participants were classified into healthy control, hypertensive and diabetic groups, the lipid levels and prevalence of lipid abnormalities were higher in hypertensive and diabetic groups compared to the control group. Low HDL level was the main prevalent dyslipidemia among study subjects. The prevalence of isolated hypertriglyceridemia, isolated hypercholesterolemia, and isolated low HDL-C was 24.7%, 14.7%, and 25.5%, respectively. Mixed hyperlipidemia was prevalent in 26.9% of the participants. According to the logistic regression analysis, significant associated factors of dyslipidemia were increased age, overweight, general and abdominal obesity, hypertension, diabetes and inadequate physical activity.

Conclusions

This study shows a high prevalence of dyslipidemia in Bangladeshi adults. Important risk factors of dyslipidemia are increased age, overweight, general and abdominal obesity, diabetes, hypertension and low physical activity. Our results suggest that awareness-raising programs are required to prevent and control dyslipidemia among Bangladeshi adults.