bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-cell RNA sequencing identifies a novel proliferation cell type affecting clinical outcome of pancreatic ductal adenocarcinoma
Authors: Ye Bicheng , Wang Qi , Zhu Xiaofeng , Zeng Lingling , Luo Huiyuan , Xiong Yan , Li Qin , Zhu Qinmei , Zhao Songyun , Chen Ting , Xie Jingen
Published: August 2023

Background

Pancreatic ductal adenocarcinoma (PDAC) is an extremely deadly neoplasm, with only a 5-year survival rate of around 9%. The tumor and its microenvironment are highly heterogeneous, and it is still unknown which cell types influence patient outcomes.

Methods

We used single-cell RNA sequencing (scRNA-seq) and spatial transcriptome (ST) to identify differences in cell types. We then applied the scRNA-seq data to decompose the cell types in bulk RNA sequencing (bulk RNA-seq) data from the Cancer Genome Atlas (TCGA) cohort. We employed unbiased machine learning integration algorithms to develop a prognosis signature based on cell type makers. Lastly, we verified the differential expression of the key gene LY6D using immunohistochemistry and qRT-PCR.

Results

In this study, we identified a novel cell type with high proliferative capacity, Prol, enriched with cell cycle and mitosis genes. We observed that the proportion of Prol cells was significantly increased in PDAC, and Prol cells were associated with reduced overall survival (OS) and progression-free survival (PFS). Additionally, the marker genes of Prol cell type, identified from scRNA-seq data, were upregulated and associated with poor prognosis in the bulk RNA-seq data. We further confirmed that mutant KRAS and TP53 were associated with an increased abundance of Prol cells and that these cells were associated with an immunosuppressive and cold tumor microenvironment in PDAC. ST determined the spatial location of Prol cells. Additionally, patients with a lower proportion of Prol cells in PDAC may benefit more from immunotherapy and gemcitabine treatment. Furthermore, we employed unbiased machine learning integration algorithms to develop a Prol signature that can precisely quantify the abundance of Prol cells and accurately predict prognosis. Finally, we confirmed that the LY6D protein and mRNA expression were markedly higher in pancreatic cancer than in normal pancreatic tissue.

Conclusions

In summary, by integrating bulk RNA-seq and scRNA-seq, we identified a novel proliferative cell type, Prol, which influences the OS and PFS of PDAC patients.
Efficacy and safety of Tripterygium wilfordii multiglucoside for idiopathic membranous nephropathy: a systematic review with bayesian meta-analysis
Authors: Wang Hongyun , Liu Hongyan , Xue Xue , Wang Qiong , Yuan Jun
Published: August 2023

Background: Currently, the optimal therapy plan for idiopathic membranous nephropathy (IMN) remains controversial as there has been no comprehensive and systematic comparison of therapy plans for IMN. Therefore, in this study, a Bayesian meta-analysis was used to systematically evaluate the clinical efficacy and safety of various intervention plans involving traditional Chinese medicine TWM in the treatment of IMN.

Methods: An electronic search in 7 databases was conducted from their inception to August 2022 for all published randomized controlled trials (RCTs) of various intervention plans for IMN. Network meta-analysis (NMA) was performed by using software R, and the surface under the cumulative ranking area (SUCRA) probability curve was plotted for each outcome indicator to rank the efficacy and safety of different intervention plans.

Results: A total of 30 RCTs were included, involving 13 interventions. The results showed that (1) in terms of total remission (TR), ① GC + CNI + TWM was the best effective among all plans, and the addition and subtraction plan of CNI + TWM was the best effective for IMN; ② All plans involving TWM were more effective than GG; ③ Among monotherapy plans for IMN, TWM was more effective distinctly than GC, while TWM and CNI were similarly effective; ④ Among multidrug therapy plans for IMN, the addition of TWM to previously established therapy plans made the original plans more effective; ⑤The efficacy of combining TWM with other plans was superior to that of TWM alone. (2) In terms of lowering 24 h-UTP, GC + TWM was the best effective and more effective than TWM. (3) In terms of safety, there was no statistically significant difference between all groups. However, CNI + TWM was the safest. No serious adverse events (AEs) occurred in all the included studies.

Conclusion: The addition of TWM may be beneficial to patients with IMN. It may enhance the efficacy of previously established treatment protocols without leading to additional safety risks. In particular, GC + CNI + TWM, GC + TWM, and CNI + TWM with better efficacy and higher safety can be preferred in clinical decision-making as the therapy plans for IMN.
The protective effects of sesamol and/or the probiotic, Lactobacillus rhamnosus, against aluminum chloride-induced neurotoxicity and hepatotoxicity in rats: Modulation of Wnt/β-catenin/GSK-3β, JAK-2/STAT-3, PPAR-γ, inflammatory, and apoptotic pathways
Authors: Abu-Elfotuh Karema , Selim Heba Mohammed Refat M. , Riad Omnia Karem M. , Hamdan Ahmed M. E. , Hassanin Soha Osama , Sharif Asmaa F. , Moustafa Nouran Magdy , Gowifel Ayah M.H. , Mohamed Marwa Y. A. , Atwa Ahmed M. , Zaghlool Sameh S. , El-Din Mahmoud Nour
Published: August 2023

Introduction: Aluminium (Al) is accumulated in the brain causing neurotoxicity and neurodegenerative disease like Alzheimer's disease (AD), multiple sclerosis, autism and epilepsy. Hence, attenuation of Al-induced neurotoxicity has become a “hot topic“ in looking for an intervention that slow down the progression of neurodegenerative diseases.

Objective: Our study aims to introduce a new strategy for hampering aluminum chloride (AlCl3)-induced neurotoxicity using a combination of sesamol with the probiotic bacteria; Lactobacillus rhamnosus (L. rhamnosus) and also to test their possible ameliorative effects on AlCl3-induced hepatotoxicity.

Methods: Sprague-Dawley male rats were randomly divided into five groups (n = 10/group) which are control, AlCl3, AlCl3 + Sesamol, AlCl3 + L. rhamnosus and AlCl3 + Sesamol + L. rhamnosus. We surveilled the behavioral, biochemical, and histopathological alterations centrally in the brain and peripherally in liver.

Results: This work revealed that the combined therapy of sesamol and L. rhamnosus produced marked reduction in brain amyloid-β, p-tau, GSK-3β, inflammatory and apoptotic biomarkers, along with marked elevation in brain free β-catenin and Wnt3a, compared to AlCl3-intoxicated rats. Also, the combined therapy exerted pronounced reduction in hepatic expressions of JAK-2/STAT-3, inflammatory (TNF-α, IL-6, NF-κB), fibrotic (MMP-2, TIMP-1, α-SMA) and apoptotic markers, (caspase-3), together with marked elevation in hepatic PPAR-γ expression, compared to AlCl3 -intoxicated rats. Behavioral and histopathological assessments substantiated the efficiency of this combined regimen in halting the effect of neurotoxicity.

Discussion: Probiotics can be used as an add-on therapy with sesamol ameliorate AlCl3 -mediated neurotoxicity and hepatotoxicity.
Oil palm kernel globulin antihypertensive peptides: isolation and characterization, ACE inhibition mechanisms, zinc-chelating activity, security and stability
Authors: Zhang Liangliang , Pan Ding , Shao Lihua , Zheng Yajun , Hao Wenhui , Kan Yu , Cao Jiawei , Yu Haotong , Liu Jing
Published: August 2023

Introduction: The oil palm kernel (OPK) expeller is the main byproduct of palm oil, but its utilization is limited.

Methods: To obtain angiotensin-I-converting enzyme (ACE) inhibition peptides with Zn-chelating capacity, defatted oil palm kernel globulin hydrolysates (DOPKGH) were subjected to Sephadex G-15 gel electrophoresis, reverse-phase high liquid performance chromatography, and UPLC-ESI-MS/MS analysis.

Results and discussion: Five representative oligopeptides, including Gln-Arg-Leu-Asp-Arg-Cys-Lys (QRLERCK), Leu-Leu-Leu-Gly-Val-Ala-Asn-Tyr-Arg (LLLGVANYR), Arg-Ala-Asp-Val-Phe-Asn-Pro-Arg (RADVFNPR), Arg-Val-Ile-Lys-Tyr-Asn-Gly-Gly-Gly-Ser-Gly (RVIKYNGGGSG), and Glu-Val-Pro-Gln-Ala-Tyr-Ile-Pro (EVPQAYIP), without potential toxicity and allergenicity, were identified in DOPKGH. Of these, only EVPQAYIP showed both ACE-inhibitory activity (IC50: 102.75 μmol/L) and Zn-chelating capacity (11.69 mg/g). Molecular docking and inhibition kinetics showed that EVPQAYIP was a competitive inhibitor of ACE because it could bind to Glu384, Lys511, and Gln281 (belonging to the central S1 and S2 pockets, respectively) of ACE. Moreover, EVPQAYIP affects zinc tetrahedral coordination in ACE by binding to Glu411; the amino and carboxyl groups of EVPQAYIP chelate with zinc ions. During gastrointestinal digestion, the ACE inhibitory activity of EVPQAYIP was relatively stable. Additionally, EVPQAYIP enhanced zinc stability in the intestine and exerted antihypertensive effects in spontaneous hypertensive rats. These results suggest the potential application of OPK peptides as ingredients in antihypertensive agents or zinc fortification.
Recommendations for wider adoption of clinical pharmacy in Central and Eastern Europe in order to optimise pharmacotherapy and improve patient outcomes
Authors: Urbańczyk Kamila , Guntschnig Sonja , Antoniadis Vasilis , Falamic Slaven , Kovacevic Tijana , Kurczewska-Michalak Marta , Miljković Branislava , Olearova Anna , Sviestina Inese , Szucs Attila , Tachkov Konstantin , Tiszai Zita , Volmer Daisy , Wiela-Hojeńska Anna , Fialova Daniela , Vlcek Jiri , Stuhec Matej , Hogg Anita , Scott Michael , Stewart Derek , Mair Alpana , Ravera Silvia , Lery François-Xavier , Kardas Przemysław
Published: August 2023

Clinical pharmacy as an area of practice, education and research started developing around the 1960s when pharmacists across the globe gradually identified the need to focus more on ensuring the appropriate use of medicines to improve patient outcomes rather than being engaged in manufacturing and supply. Since that time numerous studies have shown the positive impact of clinical pharmacy services (CPS). The need for wider adoption of CPS worldwide becomes urgent, as the global population ages, and the prevalence of polypharmacy as well as shortage of healthcare professionals is rising. At the same time, there is great pressure to provide both high-quality and cost-effective health services. All these challenges urgently require the adoption of a new paradigm of healthcare system architecture. One of the most appropriate answers to these challenges is to increase the utilization of the potential of highly educated and skilled professionals widely available in these countries, i.e., pharmacists, who are well positioned to prevent and manage drug-related problems together with ensuring safe and effective use of medications with further care relating to medication adherence. Unfortunately, CPS are still underdeveloped and underutilized in some parts of Europe, namely, in most of the Central and Eastern European (CEE) countries. This paper reviews current situation of CPS development in CEE countries and the prospects for the future of CPS in that region.
Droplet thermometry based on an optimized two dye two-color laser-induced fluorescence concept
Authors: Ulrich Hannah , Sigl Sophie , Möhnle Michael , Berrocal Edouard , Zigan Lars
Published: August 2023

In this work two-color laser-induced fluorescence (2c-LIF) is applied to calibrate and measure the temperature in a micrometric monodisperse ethanol droplet chain. A dye mixture of fluorescein disodium (FL) and sulforhodamine 101 (SRh) is used, in order to form a sensitive signal ratio of one temperature dependent and one independent fluorophore. Spectral and planar fluorescence detection via a fiber-coupled spectrometer and a camera system, consisting of two sCMOS cameras, is set up around the droplet chain. Additionally, absorption measurements of the dye mixture in ethanol are conducted using a photo-spectrometer to analyze the temperature sensitivity of the dyes as well as potential re-absorption and fluorescence cross-talk effects. The spectral setup allowed an investigation of the wavelength range in which morphology dependent resonances (MDR) occur at the phase boundary of the droplet. Thus, the optical filters, determining the color channels in the camera system, are chosen to avoid detection of the lasing signal and providing maximal temperature sensitivity at the same time. The calibrated signal ratios are applied in temperature measurements of evaporating heated droplets in the droplet chain, showing the cooling with larger distance from the nozzle.
Molecular mechanism of VE-cadherin in regulating endothelial cell behaviour during angiogenesis
Authors: Nan Weijin , He Yuxi , Wang Shurong , Zhang Yan
Published: August 2023

Vascular endothelial (VE)-cadherin, an endothelium-specific adhesion protein, is found in the junctions between endothelial cells (ECs). It’s crucial to maintain the homogeneity of ECs. Keeping and controlling the contact between ECs is essential. In addition to its adhesive function, VE-cadherin plays important roles in vascular development, permeability, and tumour angiogenesis. Signal transfer, cytoskeletal reconstruction, and contractile integrating, which are crucial for constructing and maintaining monolayer integrity as well as for repair and regeneration, are the foundation of endothelial cell (EC) junctional dynamics. The molecular basis of adhesion junctions (AJs), which are closely related and work with actin filaments, is provided by the VE-cadherin-catenin complex. They can activate intracellular signals that drive ECs to react or communicate structural changes to junctions. An increasing number of molecules, including the vascular endothelial growth factor receptor 2 (VEGFR2) and vascular endothelial protein tyrosine phosphatase (VE-PTP), have been connected to VE-cadherin in addition to the conventional VE-cadherin-catenin complex. This review demonstrates significant progress in our understanding of the molecular mechanisms that affect VE-cadherin’s function in the regulation of EC behaviour during angiogenesis. The knowledge of the molecular processes that control VE-cadherin’s role in the regulation of EC behaviour during angiogenesis has recently advanced, as shown in this review.
Leaf morphology, optical characteristics and phytochemical traits of butterhead lettuce affected by increasing the far-red photon flux
Authors: Van de Velde Ellen , Steppe Kathy , Van Labeke Marie-Christine
Published: August 2023

Light and its spectral characteristics are crucial for plant growth and development. The far-red photon flux mediates many plant processes through the action of phytochrome and also accelerates the photosynthetic electron transfer rate. In this study, we assessed the effects of far-red addition on butterhead lettuce morphology, light use efficiency, optical properties, and phytochemical characteristics. Three-week-old lettuce plants (Lactuca sativa L. cv. Alyssa) were grown for up to 28 days under a 10% blue and 90% red light spectrum (200 µmol m-2 s-1, 16 h photoperiod) to which five different intensities of far-red light (peak at 735 nm) were added (0-9-18-36-72 µmol m-2 s-1). White light-emitting diodes were included as a proxy for sunlight. Increasing supplemental far-red photon flux from zero to 21% increased the light use efficiency (g per mol) by 37% on day 14; 43% on day 21; and 39% on day 28. Measurements of projected head area suggest that this was associated with an increase in leaf expansion and photon capture and not necessarily a direct effect on photosynthesis. Moreover, vegetation indices based on leaf reflectance showed a decrease in chlorophyll-related indices under a high far-red photon flux. This decrease in pigment content was confirmed by chemical analyses, suggesting that the plants may not reach their full potential in terms of photon capture, limiting the overall photosynthetic performance. Furthermore, the stress-related Carter 1 index increased in plants grown under a high far-red photon flux, indicating early plant stress. Far-red tended to decrease the content of total phenolics and increase soluble sugars. The higher sugar levels can be attributed to an improved photochemical efficiency due to photosystem I excitation by far-red wavelengths, also known as the Emerson Enhancement effect. Despite these higher sugar levels, no effect on foliar nitrate content was observed. Our results show that far-red supplementation has the potential to enhance light interception at the early growth stages, although higher intensities of far-red may cause plant stress.
Plant-produced RBD and cocktail-based vaccine candidates are highly effective against SARS-CoV-2, independently of its emerging variants
Authors: Mamedov Tarlan , Yuksel Damla , Gurbuzaslan Irem , Ilgin Merve , Gulec Burcu , Mammadova Gulshan , Ozdarendeli Aykut , Pavel Shaikh Terkis Islam , Yetiskin Hazel , Kaplan Busra , Uygut Muhammet Ali , Hasanova Gulnara
Published: August 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel and highly pathogenic coronavirus that caused an outbreak in Wuhan City, China, in 2019 and then spread rapidly throughout the world. Although several coronavirus disease 2019 (COVID-19) vaccines are currently available for mass immunization, they are less effective against emerging SARS-CoV-2 variants, especially the Omicron (B.1.1.529). Recently, we successfully produced receptor-binding domain (RBD) variants of the spike (S) protein of SARS-CoV-2 and an antigen cocktail in Nicotiana benthamiana, which are highly produced in plants and elicited high-titer antibodies with potent neutralizing activity against SARS-CoV-2. In this study, based on neutralization ability, we demonstrate that plant-produced RBD and cocktail-based vaccine candidates are highly effective against SARS-CoV-2, independently of its emerging variants. These data demonstrate that plant-produced RBD and cocktail-based proteins are the most promising vaccine candidates and may protect against Delta and Omicron-mediated COVID-19. This is the first report describing vaccines against SARS-CoV-2, which demonstrate significant activities against Delta and Omicron variants.
Exogenous BR delayed peach fruit softening by inhibiting pectin degradation enzyme genes
Authors: Li Jianzhao , Guo Tingting , Guo Meiling , Dai Xiaonan , Xu Xiaofei , Li Yanju , Song Zhizhong , Liang Meixia
Published: August 2023

Peach fruit deteriorates and senesces rapidly when stored at room temperature. Brassinosteroids (BRs) play an important role in regulating plant growth and development and maintaining fruit quality. However, little information is available on the effect of BRs on the senescence of harvested peach fruit. In this study, different concentrations of BR were used to treat ‘Hongniang’ peach fruit, and the results showed that 10 μM BR was the most beneficial concentration to delay the senescence of peach fruits. BR treatment delayed the decrease of fruit firmness, the release of ethylene, the increase in water-soluble pectin (WSP) and ionic-soluble pectin (ISP) content and the decrease in covalently bound pectin (CBP) content, inhibited the activities of pectin degradation enzymes, and inhibited the gene expression of PpPME1/3, PpPG, PpARF2, and PpGAL2/16. In addition, BR treatment also inhibited the expression of PpBES1-5/6. Cis-acting regulatory element analysis of pectin degradation enzyme promoters showed that many of them contained BES1 binding elements. All the above results showed that BR treatment had a positive effect on delaying the senescence of peach fruit and prolonging its storage period.
The effect of brief digital mindfulness-based interventions on increasing online charitable behavior in Chinese adolescents
Authors: Hong Mei , Liang Dapeng , Lu Teng , Liu Shengchen
Published: August 2023

Digital mindfulness-based interventions (d-MBIs) have garnered significant research interest in recent years due to their psychological benefits. However, little is known about their impact on prosocial behaviors. This study investigates how d-MBIs impact prosocial behaviors where time spent is money, with Chinese adolescents as the subjects, through an online charity task (www.freerice.com). 119 students from a high school in China, who were inexperienced with mindfulness meditation, participated in this randomized controlled trial. The d-MBI group (N = 39) received online MBI guidance, while the face-to-face mindfulness-based intervention (f-MBI, N = 43) group underwent mindfulness intervention under personal tutors. The active control group (N = 37) completed a crossword task. Data analysis first involved repeated measures variance analysis, including pre-and post-intervention assessments. Subsequently, a two-way variance analysis was performed, with gender (female and male) and group (d-MBI, f-MBI, active control) as independent variables and the number of grains as dependent variables for the three groups of participants. Results showed that d-MBIs effectively improved empathy and compassion in Chinese adolescents, leading to increased rice donations to the United Nations World Food Program. These results underscore the positive effect of d-MBIs on prosociality and suggest their applicability in beneficial real-world situations involving prosocial behaviors, extending beyond previous research primarily conducted in artificial and hypothetical scenarios.
The impact of food insecurity on receipt of care, retention in care, and viral suppression among people living with HIV/AIDS in the United States: a causal mediation analysis
Authors: Bleasdale Jacob , Liu Yu , Leone Lucia A. , Morse Gene D. , Przybyla Sarahmona M.
Published: August 2023

Introduction

Attaining The Joint United Nations Programme on HIV/AIDS 95-95-95 targets to end the HIV epidemic by 2030 will require a greater understanding of the underlying mechanisms influencing care engagement among people living with HIV/AIDS (PLWHA). One such mechanism is food insecurity, defined as limited or uncertain access to food. Food insecurity has been shown to significantly impact HIV outcomes. Yet, few studies have examined the mechanisms through which food insecurity may influence these outcomes. We aimed to examine the effects of nutritional, behavioral, and mental health mechanisms through which food insecurity may impact HIV care continuum outcomes: receipt of care, retention in care, and viral suppression.

Methods

We conducted a cross-sectional study of 200 PLWHA in New York State, United States from May-August 2022. Participants were recruited using convenience sampling methods. Multivariable logistic regression models were conducted to examine the associations between food insecurity and care continuum outcomes (receipt of care, retention in care, viral suppression), adjusting for age, race, ethnicity, education, income, and marital status. Individual causal mediation analyses were conducted to assess whether behavioral, mental health, and nutritional mechanisms mediated the hypothesized associations.

Results

The median age of participants was 30 years (IQR: 27-37 years). The majority self-identified as Black (54.0%), male (55.5%) and straight/heterosexual (63.0%). Increasing severity of food insecurity was associated with greater odds of non-retention in care (aOR: 1.35, 95% CI: 1.07, 1.70) and viral non-suppression (aOR: 1.29, 95% CI: 1.08, 1.54). For the impact of food insecurity on non-retention in care, there was an indirect relationship (natural indirect effect; NIE) mediated through Body Mass Index (BMI) (ORNIE: 1.08, 95% CI: 1.00, 1.18). For viral non-suppression, there was an indirect relationship mediated through BMI (ORNIE: 1.07, 95% CI: 1.00,1.16) and an indirect relationship mediated through depression (ORNIE: 1.27, 95% CI: 1.07, 1.47).

Discussion

Food insecurity was associated with greater odds of non-retention in care and viral non-suppression among PLWHA. Nutritional and mental health pathways are important mediators of these relationships. Results highlight the need for interventions to target these pathways to address food insecurity as an underlying mechanism influencing engagement in HIV care.
Predicting ward transfer mortality with machine learning
Authors: Lezama Jose L. , Alterovitz Gil , Jakey Colleen E. , Kraus Ana L. , Kim Michael J. , Borkowski Andrew A.
Published: August 2023

In order to address a long standing challenge for internal medicine physicians we developed artificial intelligence (AI) models to identify patients at risk of increased mortality. After querying 2,425 records of patients transferred from non-intensive care units to intensive care units from the Veteran Affairs Corporate Data Warehouse (CDW), we created two datasets. The former used 22 independent variables that included “Length of Hospital Stay” and “Days to Intensive Care Transfer,” and the latter lacked these two variables. Since these two variables are unknown at the time of admission, the second set is more clinically relevant. We trained 16 machine learning models using both datasets. The best-performing models were fine-tuned and evaluated. The LightGBM model achieved the best results for both datasets. The model trained with 22 variables achieved a Receiver Operating Characteristics Curve-Area Under the Curve (ROC-AUC) of 0.89 and an accuracy of 0.72, with a sensitivity of 0.97 and a specificity of 0.68. The model trained with 20 variables achieved a ROC-AUC of 0.86 and an accuracy of 0.71, with a sensitivity of 0.94 and a specificity of 0.67. The top features for the former model included “Total length of Stay,” “Admit to ICU Transfer Days,” and “Lymphocyte Next Lab Value.” For the latter model, the top features included “Lymphocyte First Lab Value,” “Hemoglobin First Lab Value,” and “Hemoglobin Next Lab Value.” Our clinically relevant predictive mortality model can assist providers in optimizing resource utilization when managing large caseloads, particularly during shift changes.
Health System Transformation Playbook and Unified Care Model: an integrated design, systems & complexity thinking approach to health system transformation
Authors: Teo Ken Wah , Hu Yun , Chew Kwee Tiang , Pek Wee Yang , Chua Hong Choon , Matchar David Bruce , Ng Yeuk Fan
Published: August 2023

Health system transformation is a complex journey that often results in unintended consequences. Existing methods to drive health system transformation have intrinsic limitations which impede successful implementation in local contexts. The Health System Transformation Playbook is a design-, systems-, and complexity-thinking enabled methodology to systematically design, prioritize and test health system and services transformation actions, anchored on iterative story telling, model building and pathfinding processes that tackles the scale of socially and technologically complex adaptive systems through time. The Unified Care Model and its associated cascade of models are examples of ongoing application of Health System Transformation Playbook in a regional population health system in Singapore. Use of Health System Transformation Playbook enables stewards of health systems to gain a more systematic and coherent understanding of health systems and services planning and organization development, to accelerate transformation towards people-centered, integrated and value-driven health systems.
In vivo exposure to bisphenol F induces oxidative testicular toxicity: role of Erβ and p53/Bcl-2 signaling pathway
Authors: Odetayo Adeyemi Fatai , Adeyemi Wale Johnson , Olayaki Luqman Aribidesi
Published: August 2023

Introduction

Bisphenol F (BPF), an alternative to bisphenol A has been implicated as a gonadotoxic substance. BPF has been shown to induce hormonal imbalance and testicular oxidative damage. However, the mechanism associated with BPF-induced testicular toxicity has not been fully explored. This study was designed to explore the role of tumor protein (p53)/ B-cell lymphoma 2 (BCl-2) signaling and oestrogen receptor beta (Erβ) in BPF-induced testicular toxicity.

Methods

Male Wistar rats were randomized into control (Cntrl), BPF-treated (10, 30, and 50 mg/kg for low dose (BPF-L), medium dose (BPF-M), and high dose (BPF-H) respectively), and BPF-treated recovery (Cntrl-R, BPF-L-R, BPF-M-R, and BPF-H-R). The administration was via gavage and lasted for 28 days and the animals in the recovery groups were allowed 28-days exposure free period for recovery from BPF exposure.

Results

BPF resulted in the distortion of the testicular histoarchitecture, which was accompanied by a significant rise in testicular gamma-lutamyl transferase and lactate dehydrogenase activities but a decline in sorbitol dehydrogenase activities. Also, BPF caused a significant reduction in plasma gonadotropin-releasing hormone, luteinising hormone, follicle-stimulating hormone, and testosterone, which was associated with the downregulation of testicular 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase activities. Furthermore, BPF induced testicular inflammation, redox imbalance, and apoptosis, accompanied by distortion in p53/BCl-2 signaling and overexpression of Erβ. Again, the observed toxic effects of BPF were dose-dependent and not completely reversed by BPF cessation.

Discussion

Bisphenol F induced gonadotoxicity by distorting p53/BCl2 signaling and the expression of Erβ. These observed alterations were not completely reversed after the cessation of BPF exposure.
When Familiar Faces Seem Unknown: Face Blindness
Authors: Johnsamson Jordan , Tovar Elicenda , Osorio Celeste Gonzalez , Osier Nico
Published: August 2023

Think about the people in your life whose faces you could pick out in a crowd. It might surprise you to learn that some people are unable to recognize faces—even the faces of people they see often like their friends, family, and teachers. This happens with a condition called prosopagnosia, which is also known as face blindness. Prosopagnosia affects one’s ability to understand facial expressions, judge someone’s age or gender, and follow where people are looking. The fusiform face area (FFA) is a part of the brain that is responsible for facial recognition. When the FFA is underdeveloped or becomes damaged, it can lead to prosopagnosia. Currently, there is no treatment for prosopagnosia, but people affected by this condition can practice relying on other cues like sounds and smells to help them recognize others. Researchers are looking into treatment options for people with prosopagnosia.
Types of gambling: finnish gambling narratives under the lens of systems theory
Authors: Egerer Michael
Published: August 2023

There seems to be no shortage of gambling and problem gambling typologies. At a closer look, however, previous research identified types of problem gamblers and not of problem gambling. While correct typologies of gamblers are important for developing treatment, they are less useful for harm prevention. The current study uses a system theoretical approach to investigate gambling communication in order to develop a genuine typology of gambling. Snowball sampling of Finnish gamblers resulted in 56 participants, who wrote 48 narratives about their ordinary gambling, 43 narratives about their most remarkable gambling event, and 28 about their worst gambling experience. The approach is informed by systems theory: communication on gambling is understood as a result of the reduction of contingency. Rather than focusing on the meaning of gambling or why people gamble, the analysis investigates what is included and what is excluded to make gambling discussable, i.e., the contextures of gambling. Economic and family/intimate contexture were the most prominent. The latter appeared most often in the most memorable gambling experiences. The economic contexture was more common in narrating ordinary and worst gambling situations. In all, four types of gambling could be identified: genuine monetary gambling, resonating monetary gambling, commensal gambling, and liminal gambling. When comparing the previously identified types of gamblers with the types of gambling discovered in the present study, it becomes obvious that a shift from the gamblers, their background, their personality, and their motives to the gambling activity provides novel insights. The constant appearance of the familial/intimate dimension in the narratives indicates that, beside the financial harms, societal harms also need to be treated as a category of harm in its own right, not just as a consequence of personality disorders, psychological distress, or social deprivation.
Optimizing phosphorus management to increase grain yield and nutritional quality while reducing phytic acid concentration in black rice (Oryza sativa L.)
Authors: Oo Aung Zaw , Asai Hidetoshi , Kawamura Kensuke , Marui Junichiro , Nakahara Kazuhiko , Takai Toshiyuki , Saito Hiroki , Win Khin Thuzar , Pariasca-Tanaka Juan
Published: August 2023

Rice with a black-colored pericarp (hereafter, black rice) has attracted considerable attention because of its high nutritional value. It is traditionally grown in infertile agroecosystems, where soil nutrient deficiency would affect grain yield and nutritional quality of the rice. This study examined the effects of phosphorus (P) application (0, 100, 250, 500, 1,000, and 1,300 mg P2O5 pot−1) on the growth, grain yield, and certain quality parameters of unpolished black (“Asamurasaki”) and white (“Takanari”) rice. The results showed that black rice grew poorly under P limitation, resulting in fewer tillers and a lower grain yield, while maintaining higher photosynthetic efficiency than white rice. However, black rice produced under P stress exhibited higher levels of Zn, Fe, and antioxidant (total phenolic and flavonoid content) than white rice, making it more nutritious. The optimal P application rates for black and white rice were 250 and 500 mg P2O5 pot−1, respectively, to achieve high yields. At the optimal rate, black rice exhibited high Fe, Zn and antioxidant levels compared to that at higher P application rates and had lower phytic acid levels, which could alleviate its inhibition of mineral absorption in the digestive tract. Moreover, low grain P due to P stress decreased the lightness L* value in the CIE-Lab system, improving the visual grain quality. Therefore, optimal P management is crucial for maintaining optimal grain yield with low phytic acid concentration and enhancing the nutritional quality of black rice for human consumption.
Comparison of ruminal microbiota, metabolomics, and milk performance between Montbéliarde×Holstein and Holstein cattle
Authors: Chang Haomiao , Wang Xinling , Zeng Hanfang , Zhai Yunfei , Huang Ni , Wang Changjian , Han Zhaoyu
Published: August 2023

Holstein cattle are well known for their high average milk yield but are more susceptible to disease and have lower fecundity than other breeds of cattle. The purpose of this study was to explore the relationship between ruminal metabolites and both milk performance and ruminal microbiota composition as a means of assessing the benefits of crossbreeding Montbéliarde and Holstein cattle. This experiment crossbred Holstein with Montbéliarde cattle, aimed to act as a reference for producing high-quality dairy products and improving the overall efficiency of dairy cattle breeding. Based on similar age, parity and lactation time, 46 cows were selected and divided into two groups (n  =  23 per group) for comparison experiment and fed the same formula: Montbéliarde×Holstein (MH, DIM  =  33.23  ±  5.61 d), Holstein (H, DIM  =  29.27  ±  4.23 d). Dairy herd improvement (DHI) data is an important basis for evaluating the genetic quality of bulls, understanding the quality level of milk, and improving feeding management. We collected the DHI data of these cows in the early lactation, middle lactation and late lactation period of 10  months. The results showed that the average milk production and protein content in Montbéliarde×Holstein were 1.76  kg (34.41  kg to 32.65  kg, p  >  0.05) and 0.1% (3.54 to 3.44%, p  <  0.05) higher than in Holstein cattle. Moreover, milk from Montbéliarde×Holstein cattle had lesser somatic cell score (1.66 to 2.02) than Holstein cattle (p  <  0.01). A total of 10 experimental cattle in early lactation were randomly selected in the two groups (Lactation time  =  92.70  ±  6.81), and ruminal fluid were collected by oral gastric tube. Using 16S rRNA microbial sequencing, we compared the ruminal microbiota composition and found that Montbéliarde×Holstein cattle had a lower abundance of Alphaproteobacteria (p  <  0.05) and higher abundance of Selenomonas than Holstein cattle (p  <  0.05). These bacteria play roles in protein degradation, nitrogen fixation and lactic acid degradation. The abundance of Succiniclasticum was also greater in Montbéliarde×Holstein cattle (p  =  0.053). Through ruminal metabolome analysis, we found that the levels of trans-ferulic acid, pyrrole-2-carboxylic acid, and quinaldic acid were significantly increased in Montbéliarde×Holstein cattle, while that of lathosterol was significantly decreased. The changes in the levels of these metabolites could confer improved antioxidant, anti-inflammatory, and antibacterial activities.
Choice, control, and animal welfare: definitions and essential inquiries to advance animal welfare science
Authors: Englund Maisy D. , Cronin Katherine A.
Published: August 2023

The ways in which humans can support good welfare for animals in their care is an ongoing subject of debate: some place emphasis on the animals’ physical health; others, on animals’ ability to live “natural lives”; and others on animals’ affective states or psychological wellbeing. Recently, there has been an increase in interest in how an animal’s ability to exercise control over its environment could impact their welfare. In this article, we take the stance that the relevant aspects of the first two concepts of animal welfare (physical health, natural lives) are largely addressed when an animal’s psychological wellbeing is prioritized. Through that lens, we review the current state of the literature regarding the psychology of control, and the intersection between choice, control, and welfare. We clarify terms to support future work, and propose future directions that could lead to a better understanding of the psychological benefits of choice and control and ultimately inform animal care decisions.
Development and Performance Evaluation of Low-Cost 2WT-Operated Earthing-Up Machine for Sugarcane Cultivation in Bangladesh
Authors: Ferdows Md. Nafiul , Rahman Md. Anisur , Ali Md. Rostom , Hanif Md. Abu , Tabriz Sayed Shams , Hossen Md. Sanowar , Rokonuzzaman Md.
Published: August 2023

Like most crops, sugarcane needs to be kept upright until it is harvested. The lodging of sugarcane has significant negative effects on the cane yield and sugar content of sugarcane. To keep sugarcane upright, earthing up is an essential in the cultural part of the operation. In Bangladesh, most of the sugarcane cultivation operations, including earthing-up, are generally performed in a traditional manual method which increases the production costs as well as reduces the income of sugarcane growers. Therefore, a cost-effective two-wheeled tractor (2WT)-mounted earthing-up machine was developed at the Bangladesh Sugarcrop Research Institute (BSRI), Pabna, to reduce drudgery and the cost of sugarcane production. Field tests were conducted in an experimental sugarcane field at BSRI and technical and economic performances of the developed earthing-up machine were also carried out based on the field test. The average effective field capacity and field efficiency of the earthing-up machine were found to be 0.16 ha/h and 77.41%, respectively. The 2WT-driven earthing-up machine was not found to be economically viable when it was used only for earthing-up operations. However, when the 2WT was used as the main driver for other activities, including earthing-up operation, the earthing-up machine became economically beneficial with net cash flow (NCF), net present value (NPV), internal rate of return (IRR), benefit–cost ratio (BCR), and payback period (PP) of BDT 148,497/ha, BDT 23,184, 3%, 3.81:1, and approximately 1 year, respectively. On the contrary, considering the cost of only earthing-up tool without 2WT, it was found to be economically beneficial with NCF, NPV, IRR, BCR, and PP of BDT 16,428/ha, BDT 3053, 4.7%, 2.71:1, and approximately 2 years, respectively. In Bangladesh, 2WT is commonly used for versatile farming purposes. Therefore, the versatile use of 2WT as a prime mover for other machines, including the earthing-up machine, can make earthing-up machine economically viable and beneficial for sugarcane growers in Bangladesh.
A Best-Bet System of Rice Intensification for Sustainable Rice (Oryza sativa L.) Production in Northwestern Nigeria
Authors: Siéwé François , Egwuma Henry , Sanni Adunni , Ahmed Ben , Abu Sunday T. , Nwahia Cordelia O. , Fani Djomo Choumbou Raoul , Abdulkadir Aisha , Ogunsola Elijah O.
Published: August 2023

System of Rice Intensification (SRI) practices are expected to be used in location-specific ways and thus will vary somewhat across countries and regions. This study undertook to identify a ‘best-bet’ version of SRI for conditions in northwestern Nigeria, considering what is feasible for farmer use. Two years of experimental data from 260 farmer-managed rice plots evaluating four of the SRI practices in Zamfara State were analyzed. The variables evaluated were seedling age at transplanting, plant density, irrigation schedule, and fertilizer application. Farm budget analysis showed that the best-bet SRI practices most productive given the natural environment and farming system were transplanting 11-day-old seedlings at 25 cm × 25 cm spacing, with alternate wetting-and-drying of fields, as well as providing full compost plus some inorganic fertilization. Net economic returns were found to be highest with best-bet SRI practices and ranged from USD 1450–2120 ha−1. While rice production was profitable under both SRI and more conventional management, the return on investment was at least 40% higher with SRI practices than with the other practices evaluated. Based on our data and analysis, we recommend that the Nigerian government and its development partners prioritize and expand the testing and promotion of SRI in the northwest and other regions of the country. This initiative can significantly enhance farmers’ incomes and, ultimately, bolster food security.
Chloroplast Damage and Photosynthetic System Disorder Induced Chlorosis in the Leaves of Rice Seedlings under Excessive Biuret
Authors: Zhang Peng , Chen Yulin , Zhang Yuping , Xiang Jing , Wang Yaliang , Wang Zhigang , Chen Huizhe , Zhang Yikai
Published: August 2023

Excessive biuret in fertilizer causes leaf albinism in direct-seeded rice fields. This study aimed to provide a comprehensive understanding of the underlying physiology and molecular mechanisms of leaf chlorosis via biuret using morphophysiological and transcriptome analyses. The induction of biuret in albino rice leaves was examined in a net-growing cultivation bed. Some key morphophysiological indices were measured including biuret content, blade ultrastructure, chlorophyll content, and chlorophyll fluorescence parameters. Candidate genes in the chlorotic leaves under biuret stress were also excavated using transcriptome analysis. Furthermore, physiological and biochemical analyses of the changes in enzyme activities and intermediate metabolite contents in relation to the phenotypic changes in the leaves were carried out. The chlorotic leaves of rice seedlings showed higher biuret accumulation, and the leaves suffered severe damage with higher malondialdehyde contents and low chlorophyll contents. Abnormal chloroplast ultrastructures and thylakoid membrane structure loss were observed in chlorotic leaves under biuret exposure. The related genes involved in the chloroplast development, photosynthesis (including antenna proteins), and carbon fixation pathways were significantly downregulated, which suggests that photosynthesis was destroyed in the chlorotic leaves of rice seedlings. Biuret disturbed the photosynthetic system in chloroplast thylakoid membranes by inhibiting chloroplast development, thereby promoting the formation of the chlorotic leaf phenotype in rice seedlings. Our results promote the understanding of the molecular mechanism of rice in response to biuret toxicity.
Contribution of Glutathione Transferases in the Selective and Light-Dependent Effect of Flumioxazin on Winter Wheat (Triticum aestivum L.) and Its Typical Weed Common Poppy (Papaver rhoeas L.)
Authors: Gallé Ágnes , Farkas Máté , Pelsőczi Alina , Czékus Zalán , Kukri András , Dorner Zita , Ördög Attila , Csiszár Jolán , Bela Krisztina , Poór Péter
Published: August 2023

Glutathione transferases (GSTs) are enzymes that catalyse modifications and conjugations of a range of organic and often cytotoxic compounds. GST enzymes with many functions—such as their conjugation activity against herbicides and their metabolites—can be induced and show light and circadian determination. The enzyme family, which is widespread in its function, also shows great diversity in its structure, which has been linked to its enzyme kinetic characteristics and physiological role at many points. In this study, we aimed to find out the role of different glutathione transferases in the herbicide responses to flumioxazin, as well as to determine how the antioxidant and detoxification response to herbicide treatment changes in the presence and absence of light. One of the herbicide treatments was carried out during the light period in the morning (9:00 a.m.), and the other before the end of the dark period (4:00 a.m.). The decrease in the maximal quantum efficiency of PS II and the reduction in the chlorophyll concentration supported the effect of the herbicide on Papaver rhoeas. In the guaiacol peroxidase POD and GST activity, there were large differences between the cultivated plants and the weed; both enzyme activities were much higher in the case of wheat. According to the activity of the antioxidant defence enzymes and GST gene expression data, the application of the photosynthesis inhibitor herbicide, flumioxazin, in the dark could allow the wheat antioxidant defence to switch on before the herbicide effect could appear in the light period. Phi and tau group GSTs were transcriptionally upregulated by the treatments in wheat plants (especially TaGSTU1B), while fewer changes were detectable in poppy weed (PrGSTU4). Based on our results, in the background of the greater and more successful response to flumioxazin may be—among other things—the higher degree of variability of the GSTU genes of wheat compared to poppies.