bluuebunny/crossref_metadata_2025 huggingface dataset:
Between race and animality: European borders, ‘colonial dogs’, and the policing of humanity
Authors: Brito Tarsis
Published: February 2024

Europe’s (post-)colonial borders have been recently marked by a profusion of cases of violence against racialised migrants with the use of police dogs, following a continual process of integration of canines into the border apparatus of violence. Engaging simultaneously with the recent post-colonial literature on border and migration security and the incipient domain of animal studies, this article investigates the colonial and racial origins and effects of this phenomenon. Contextualising the weaponisation of dogs at Europe’s borders today within a much longer history of racial violence, the article shows how canines have been systematically deployed by colonial and white supremacist powers against racialised bodies as tools to enact and secure racial order. Attentive to the ways in which modern humanness has been predicated upon its removal from the food chain, the article argues that the use of police dogs at Europe’s borders operates by reinforcing the non- or less-than-human status of racialised migrants by marking them as ‘animal-like’ and ‘edible’ bodies. Conceptualising this method as ‘the politics of edibility’, the article then shows how the exposure of migrants to the threat of ‘dog bites’ functions as a form of reinforcing racial hierarchies in a Europe traversed by racial anxieties.
Non‐Thermal Oxygen Escape on Mars in the Presence of Gravity Waves
Authors: Gu Hao , Cui Jun , Wang Xing , Huang Xu , Zhao Jin‐Jin , Wu Zhao‐Peng , Li Lei
Published: February 2024

Extensive measurements made over the past two decades have indicated the widespread and frequent occurrence of gravity waves in the atmosphere of Mars. Gravity waves are able to significantly modify the atmospheric structure and potentially affect atmospheric escape. This study is devoted to examining the hot O escape variability on Mars in the presence of gravity waves with the aid of the Wentzel–Kramers–Brillouin approximation and the multi collision model as well as the multi‐instrument MAVEN data set. Our calculations suggest that the hot O escape probability tends to be enhanced or suppressed in the presence of gravity waves near the Martian exobase and the impacts vary substantially with the ejection angle and nascent energy of hot O, and gravity wave characteristics. Further study indicates that although gravity waves play a negligible role in the averaged hot O escape probability, they are able to enhance hot O escape flux by 20% via altering the hot O production rate rather. Since gravity waves are omnipresent on any planetary body with a permanent atmosphere, they are expected to affect the non‐thermal escape on solar system and extrasolar bodies.
Thermal Equations of State of Magnesite: Implication for the Complex Mid‐Lower Mantle Seismic Scatterers
Authors: Yu Yingxin , Sun Ningyu , Mao Zhu , Li Luo , Prakapenka Vitali B. , Lin Jung‐Fu
Published: February 2024

Magnesite (MgCO3) entering the lower mantle together with the subducted oceanic crust is an important carbon carrier. The reaction between magnesite and mantle minerals has been documented, but its influence on the density and velocity profiles of lower mantle remains unexplored. To decipher the deep carbon transportation and its associated effect, here we determined the thermal equations of state of magnesite up to 120 GPa and 2600 K using X‐ray diffraction in laser‐heated diamond anvil cells. The obtained thermal elastic parameters of magnesite facilitated a comprehensive understanding on the influence of magnesite‐SiO2 reaction, variation of carbon and SiO2 content, and temperature on the origin of lower‐mantle scatterers at 1,000–1,800 km depth. Our modeling revealed that the depth of the lower‐mantle VS scatterers is mainly controlled by the Al2O3 content in SiO2, while its magnitude depends on the SiO2 content. Along normal geotherm, the magnesite‐SiO2 reaction would occur before the post‐stishovite transition, consuming substantial SiO2 in the subducted oceanic crust. Depending on the amount of residual SiO2, the post‐stishovite transition can produce a 2.5–5.2 (2)% VS reduction, compatible with the observed seismic scatterers in Izu‐Bonin and Mariana subduction zones. Along slab geotherm, this reaction occurs after the post‐stishovite transition, generating a greater VS reduction of 4.4–6.4 (4)%. We thus propose that the reaction between sinking MgCO3 and SiO2 in the slab is one of the potential factors influencing the magnitude of the lower‐Vs scatterers at 1,000–1,900 km depth. Our results provide new insights into the deep‐mantle carbonate transportation influencing regional geophysics.
Regulation of autophagy by perilysosomal calcium: a new player in β-cell lipotoxicity
Authors: Nguyen Ha Thu , Wiederkehr Andreas , Wollheim Claes B. , Park Kyu-Sang
Published: February 2024

Autophagy is an essential quality control mechanism for maintaining organellar functions in eukaryotic cells. Defective autophagy in pancreatic beta cells has been shown to be involved in the progression of diabetes through impaired insulin secretion under glucolipotoxic stress. The underlying mechanism reveals the pathologic role of the hyperactivation of mechanistic target of rapamycin (mTOR), which inhibits lysosomal biogenesis and autophagic processes. Moreover, accumulating evidence suggests that oxidative stress induces Ca2+ depletion in the endoplasmic reticulum (ER) and cytosolic Ca2+ overload, which may contribute to mTOR activation in perilysosomal microdomains, leading to autophagic defects and β-cell failure due to lipotoxicity. This review delineates the antagonistic regulation of autophagic flux by mTOR and AMP-dependent protein kinase (AMPK) at the lysosomal membrane, and both of these molecules could be activated by perilysosomal calcium signaling. However, aberrant and persistent Ca2+ elevation upon lipotoxic stress increases mTOR activity and suppresses autophagy. Therefore, normalization of autophagy is an attractive therapeutic strategy for patients with β-cell failure and diabetes.
Motion of charged particles in bright squeezed vacuum
Authors: Even Tzur Matan , Cohen Oren
Published: February 2024

The motion of laser-driven electrons quivers with an average energy termed pondermotive energy. We explore electron dynamics driven by bright squeezed vacuum (BSV), finding that BSV induces width oscillations, akin to electron quivering in laser light, with an equivalent ponderomotive energy. We identify closed and open trajectories of the electronic width that are associated with high harmonic generation and above-threshold ionization, respectively, similarly to trajectories of the electron position when its motion is driven by coherent light. In the case of bound electrons, the width oscillations may lead to ionization with noisy sub-cycle structure. Our results are foundational for strong-field and free-electron quantum optics, as they shed light on ionization, high harmonic generation, and nonlinear Compton scattering in BSV.
Complex-frequency waves: beat loss and win sensitivity
Authors: Cheng Qingqing , Li Tao
Published: February 2024

Recent experiments have demonstrated that synthesized complex-frequency waves can impart a virtual gain to molecule sensing systems, which can effectively restore information lost due to intrinsic molecular damping. The enhancement notably amplifies the signal of trace molecular vibrational fingerprints, thereby substantially improving the upper limit of sensitivity.
Pivotal role of IL-8 derived from the interaction between osteosarcoma and tumor-associated macrophages in osteosarcoma growth and metastasis via the FAK pathway
Authors: Tatsuno Rikito , Ichikawa Jiro , Komohara Yoshihiro , Pan Cheng , Kawasaki Tomonori , Enomoto Atsushi , Aoki Kaoru , Hayakawa Keiko , Iwata Shintaro , Jubashi Takahiro , Haro Hirotaka
Published: February 2024

The prognosis of osteosarcoma (OS) has remained stagnant over the past two decades, requiring the exploration of new therapeutic targets. Cytokines, arising from tumor-associated macrophages (TAMs), a major component of the tumor microenvironment (TME), have garnered attention owing to their impact on tumor growth, invasion, metastasis, and resistance to chemotherapy. Nonetheless, the precise functional role of TAMs in OS progression requires further investigation. In this study, we investigated the interaction between OS and TAMs, as well as the contribution of TAM-produced cytokines to OS advancement. TAMs were observed to be more prevalent in lung metastases compared with that in primary tumors, suggesting their potential support for OS progression. To simulate the TME, OS and TAMs were co-cultured, and the cytokines resulting from this co-culture could stimulate OS proliferation, migration, and invasion. A detailed investigation of cytokines in the co-culture conditioned medium (CM) revealed a substantial increase in IL-8, establishing it as a pivotal cytokine in the process of enhancing OS proliferation, migration, and invasion through the focal adhesion kinase (FAK) pathway. In an in vivo model, co-culture CM promoted OS proliferation and lung metastasis, effects that were mitigated by anti-IL-8 antibodies. Collectively, IL-8, generated within the TME formed by OS and TAMs, accelerates OS proliferation and metastasis via the FAK pathway, thereby positioning IL-8 as a potential novel therapeutic target in OS.
Roles of exosomes in immunotherapy for solid cancers
Authors: Lyu Cong , Sun Haifeng , Sun Zhenqiang , Liu Yang , Wang Qiming
Published: February 2024

Although immunotherapy has made breakthrough progress, its efficacy in solid tumours remains unsatisfactory. Exosomes are the main type of extracellular vesicles that can deliver various intracellular molecules to adjacent or distant cells and organs, mediating various biological functions. Studies have found that exosomes can both activate the immune system and inhibit the immune system. The antigen and major histocompatibility complex (MHC) carried in exosomes make it possible to develop them as anticancer vaccines. Exosomes derived from blood, urine, saliva and cerebrospinal fluid can be used as ideal biomarkers in cancer diagnosis and prognosis. In recent years, exosome-based therapy has made great progress in the fields of drug transportation and immunotherapy. Here, we review the composition and sources of exosomes in the solid cancer immune microenvironment and further elaborate on the potential mechanisms and pathways by which exosomes influence immunotherapy for solid cancers. Moreover, we summarize the potential clinical application prospects of engineered exosomes and exosome vaccines in immunotherapy for solid cancers. Eventually, these findings may open up avenues for determining the potential of exosomes for diagnosis, treatment, and prognosis in solid cancer immunotherapy.
Environment geometry alters subiculum boundary vector cell receptive fields in adulthood and early development
Authors: Muessig Laurenz , Ribeiro Rodrigues Fabio , Bjerknes Tale L. , Towse Benjamin W. , Barry Caswell , Burgess Neil , Moser Edvard I. , Moser May-Britt , Cacucci Francesca , Wills Thomas J.
Published: February 2024

Boundaries to movement form a specific class of landmark information used for navigation: Boundary Vector Cells (BVCs) are neurons which encode an animal’s location as a vector displacement from boundaries. Here we characterise the prevalence and spatial tuning of subiculum BVCs in adult and developing male rats, and investigate the relationship between BVC spatial firing and boundary geometry. BVC directional tunings align with environment walls in squares, but are uniformly distributed in circles, demonstrating that environmental geometry alters BVC receptive fields. Inserted barriers uncover both excitatory and inhibitory components to BVC receptive fields, demonstrating that inhibitory inputs contribute to BVC field formation. During post-natal development, subiculum BVCs mature slowly, contrasting with the earlier maturation of boundary-responsive cells in upstream Entorhinal Cortex. However, Subiculum and Entorhinal BVC receptive fields are altered by boundary geometry as early as tested, suggesting this is an inherent feature of the hippocampal representation of space.
The chromatin-associated lncREST ensures effective replication stress response by promoting the assembly of fork signaling factors
Authors: Statello Luisa , Fernandez-Justel José Miguel , González Jovanna , Montes Marta , Ranieri Alessia , Goñi Enrique , Mas Aina M. , Huarte Maite
Published: February 2024

Besides the well-characterized protein network involved in the replication stress response, several regulatory RNAs have been shown to play a role in this critical process. However, it has remained elusive whether they act locally at the stressed forks. Here, by investigating the RNAs localizing on chromatin upon replication stress induced by hydroxyurea, we identified a set of lncRNAs upregulated in S-phase and controlled by stress transcription factors. Among them, we demonstrate that the previously uncharacterized lncRNA lncREST (long non-coding RNA REplication STress) is transcriptionally controlled by p53 and localizes at stressed replication forks. LncREST-depleted cells experience sustained replication fork progression and accumulate un-signaled DNA damage. Under replication stress, lncREST interacts with the protein NCL and assists in engaging its interaction with RPA. The loss of lncREST is associated with a reduced NCL-RPA interaction and decreased RPA on chromatin, leading to defective replication stress signaling and accumulation of mitotic defects, resulting in apoptosis and a reduction in tumorigenic potential of cancer cells. These findings uncover the function of a lncRNA in favoring the recruitment of replication proteins to sites of DNA replication.
Tissue-specific profiling of age-dependent miRNAomic changes in Caenorhabditis elegans
Authors: Wang Xueqing , Jiang Quanlong , Zhang Hongdao , He Zhidong , Song Yuanyuan , Chen Yifan , Tang Na , Zhou Yifei , Li Yiping , Antebi Adam , Wu Ligang , Han Jing-Dong J. , Shen Yidong
Published: February 2024

Ageing exhibits common and distinct features in various tissues, making it critical to decipher the tissue-specific ageing mechanisms. MiRNAs are essential regulators in ageing and are recently highlighted as a class of intercellular messengers. However, little is known about the tissue-specific transcriptomic changes of miRNAs during ageing. C. elegans is a well-established model organism in ageing research. Here, we profile the age-dependent miRNAomic changes in five isolated worm tissues. Besides the diverse ageing-regulated miRNA expression across tissues, we discover numerous miRNAs in the tissues without their transcription. We further profile miRNAs in the extracellular vesicles and find that worm miRNAs undergo inter-tissue trafficking via these vesicles in an age-dependent manner. Using these datasets, we uncover the interaction between body wall muscle-derived mir-1 and DAF-16/FOXO in the intestine, suggesting mir-1 as a messenger in inter-tissue signalling. Taken together, we systematically investigate worm miRNAs in the somatic tissues and extracellular vesicles during ageing, providing a valuable resource to study tissue-autonomous and nonautonomous functions of miRNAs in ageing.
Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos
Authors: Kiyomitsu Ai , Nishimura Toshiya , Hwang Shiang Jyi , Ansai Satoshi , Kanemaki Masato T. , Tanaka Minoru , Kiyomitsu Tomomi
Published: February 2024

Despite drastic cellular changes during cleavage, a mitotic spindle assembles in each blastomere to accurately segregate duplicated chromosomes. Mechanisms of mitotic spindle assembly have been extensively studied using small somatic cells. However, mechanisms of spindle assembly in large vertebrate embryos remain little understood. Here, we establish functional assay systems in medaka (Oryzias latipes) embryos by combining CRISPR knock-in with auxin-inducible degron technology. Live imaging reveals several unexpected features of microtubule organization and centrosome positioning that achieve rapid, accurate cleavage. Importantly, Ran-GTP assembles a dense microtubule network at the metaphase spindle center that is essential for chromosome segregation in early embryos. This unique spindle structure is remodeled into a typical short, somatic-like spindle after blastula stages, when Ran-GTP becomes dispensable for chromosome segregation. We propose that despite the presence of centrosomes, the chromosome-derived Ran-GTP pathway has essential roles in functional spindle assembly in large, rapidly dividing vertebrate early embryos, similar to acentrosomal spindle assembly in oocytes.
Clocked dynamics in artificial spin ice
Authors: Jensen Johannes H. , Strømberg Anders , Breivik Ida , Penty Arthur , Niño Miguel Angel , Khaliq Muhammad Waqas , Foerster Michael , Tufte Gunnar , Folven Erik
Published: February 2024

Artificial spin ice (ASI) are nanomagnetic metamaterials with a wide range of emergent properties. Through local interactions, the magnetization of the nanomagnets self-organize into extended magnetic domains. However, controlling when, where and how domains change has proven difficult, yet is crucial for technological applications. Here, we introduce astroid clocking, which offers significant control of ASI dynamics in both time and space. Astroid clocking unlocks a discrete, step-wise and gradual dynamical process within the metamaterial. Notably, our method employs global fields to selectively manipulate local features within the ASI. Sequences of these clock fields drive domain dynamics. We demonstrate, experimentally and in simulations, how astroid clocking of pinwheel ASI enables ferromagnetic domains to be gradually grown or reversed at will. Richer dynamics arise when the clock protocol allows both growth and reversal to occur simultaneously. With astroid clocking, complex spatio-temporal behaviors of magnetic metamaterials become easily controllable with high fidelity.
Mechanical forces across compartments coordinate cell shape and fate transitions to generate tissue architecture
Authors: Villeneuve Clémentine , Hashmi Ali , Ylivinkka Irene , Lawson-Keister Elizabeth , Miroshnikova Yekaterina A. , Pérez-González Carlos , Myllymäki Satu-Marja , Bertillot Fabien , Yadav Bhagwan , Zhang Tao , Matic Vignjevic Danijela , Mikkola Marja L. , Manning M. Lisa , Wickström Sara A.
Published: February 2024

Morphogenesis and cell state transitions must be coordinated in time and space to produce a functional tissue. An excellent paradigm to understand the coupling of these processes is mammalian hair follicle development, which is initiated by the formation of an epithelial invagination—termed placode—that coincides with the emergence of a designated hair follicle stem cell population. The mechanisms directing the deformation of the epithelium, cell state transitions and physical compartmentalization of the placode are unknown. Here we identify a key role for coordinated mechanical forces stemming from contractile, proliferative and proteolytic activities across the epithelial and mesenchymal compartments in generating the placode structure. A ring of fibroblast cells gradually wraps around the placode cells to generate centripetal contractile forces, which, in collaboration with polarized epithelial myosin activity, promote elongation and local tissue thickening. These mechanical stresses further enhance compartmentalization of Sox9 expression to promote stem cell positioning. Subsequently, proteolytic remodelling locally softens the basement membrane to facilitate a release of pressure on the placode, enabling localized cell divisions, tissue fluidification and epithelial invagination into the underlying mesenchyme. Together, our experiments and modelling identify dynamic cell shape transformations and tissue-scale mechanical cooperation as key factors for orchestrating organ formation.
An fMRI Dataset on Social Reward Processing and Decision Making in Younger and Older Adults
Authors: Smith David V. , Ludwig Rita M. , Dennison Jeffrey B. , Reeck Crystal , Fareri Dominic S.
Published: February 2024

Behavioural and neuroimaging research has shown that older adults are less sensitive to financial losses compared to younger adults. Yet relatively less is known about age-related differences in social decisions and social reward processing. As part of a pilot study, we collected behavioural and functional magnetic resonance imaging (fMRI) data from 50 participants (Younger: N = 26, ages 18–34 years; Older: N = 24, ages 63–80 years) who completed three tasks in the scanner: an economic trust game as the investor with three partners (computer, stranger, friend) as the investee; a card-guessing task with monetary gains and losses shared with three partners (computer, stranger, friend); and an ultimatum game as responder to three anonymous proposers (computer, age-similar adults, age-dissimilar adults). We also collected B0 field maps and high-resolution structural images (T1-weighted and T2-weighted images). These data could be reused to answer questions about moment-to-moment variability in fMRI signal, representational similarity between tasks, and brain structure.
Marine picoplankton metagenomes and MAGs from eleven vertical profiles obtained by the Malaspina Expedition
Authors: Sánchez Pablo , Coutinho Felipe H. , Sebastián Marta , Pernice Massimo C. , Rodríguez-Martínez Raquel , Salazar Guillem , Cornejo-Castillo Francisco Miguel , Pesant Stéphane , López-Alforja Xabier , López-García Ester María , Agustí Susana , Gojobori Takashi , Logares Ramiro , Sala Maria Montserrat , Vaqué Dolors , Massana Ramon , Duarte Carlos M. , Acinas Silvia G. , Gasol Josep M.
Published: February 2024

The Ocean microbiome has a crucial role in Earth’s biogeochemical cycles. During the last decade, global cruises such as Tara Oceans and the Malaspina Expedition have expanded our understanding of the diversity and genetic repertoire of marine microbes. Nevertheless, there are still knowledge gaps regarding their diversity patterns throughout depth gradients ranging from the surface to the deep ocean. Here we present a dataset of 76 microbial metagenomes (MProfile) of the picoplankton size fraction (0.2–3.0 µm) collected in 11 vertical profiles covering contrasting ocean regions sampled during the Malaspina Expedition circumnavigation (7 depths, from surface to 4,000 m deep). The MProfile dataset produced 1.66 Tbp of raw DNA sequences from which we derived: 17.4 million genes clustered at 95% sequence similarity (M-GeneDB-VP), 2,672 metagenome-assembled genomes (MAGs) of Archaea and Bacteria (Malaspina-VP-MAGs), and over 100,000 viral genomic sequences. This dataset will be a valuable resource for exploring the functional and taxonomic connectivity between the photic and bathypelagic tropical and sub-tropical ocean, while increasing our general knowledge of the Ocean microbiome.
DEBBIES Dataset to study Life Histories across Ectotherms
Authors: Smallegange Isabel M. , Lucas Sol
Published: February 2024

Demographic models are used to explore how life history traits structure life history strategies across species. This study presents the DEBBIES dataset that contains estimates of eight life history traits (length at birth, puberty and maximum length, maximum reproduction rate, fraction energy allocated to respiration versus reproduction, von Bertalanffy growth rate, mortality rates) for 185 ectotherm species. The dataset can be used to parameterise dynamic energy budget integral projection models (DEB-IPMs) to calculate key demographic quantities like population growth rate and demographic resilience, but also link to conservation status or biogeographical characteristics. Our technical validation shows a satisfactory agreement between observed and predicted longevity, generation time, age at maturity across all species. Compared to existing datasets, DEBBIES accommodates (i) easy cross-taxonomical comparisons, (ii) many data-deficient species, and (iii) population forecasts to novel conditions because DEB-IPMs include a mechanistic description of the trade-off between growth and reproduction. This dataset has the potential for biologists to unlock general predictions on ectotherm population responses from only a few key life history traits.
Survey of selected viral agents (herpesvirus, adenovirus and hepatitis E virus) in liver and lung samples of cetaceans, Brazil
Authors: Sacristán C. , Ewbank A. C. , Duarte-Benvenuto A. , Sacristán I. , Zamana-Ramblas R. , Costa-Silva S. , Lanes Ribeiro V. , Bertozzi C. P. , del Rio do Valle R. , Castilho P. V. , Colosio A. C. , Marcondes M. C. C. , Lailson-Brito J. , de Freitas Azevedo A. , Carvalho V. L. , Pessi C. F. , Cremer M. , Esperón F. , Catão-Dias J. L.
Published: February 2024

Hepatic and pulmonary lesions are common in cetaceans, despite their poorly understood viral etiology. Herpesviruses (HV), adenoviruses (AdV) and hepatitis E virus (HEV) are emerging agents in cetaceans, associated with liver and/or pulmonary damage in mammals. We isolated and molecularly tested DNA for HV and AdV (n = 218 individuals; 187 liver and 108 lung samples) and RNA for HEV (n = 147 animals; 147 liver samples) from six cetacean families. All animals stranded or were bycaught in Brazil between 2001 and 2021. Positive-animals were analyzed by histopathology. Statistical analyses assessed if the prevalence of viral infection could be associated with the variables: species, family, habitat, region, sex, and age group. All samples were negative for AdV and HEV. Overall, 8.7% (19/218) of the cetaceans were HV-positive (4.8% [9/187] liver and 11.1% [12/108] lung), without HV-associated lesions. HV-prevalence was statistically significant higher in Pontoporiidae (19.2%, 10/52) when compared to Delphinidae (4.1%, 5/121), and in southeastern (17.1%, 13/76)—the most industrialized Brazilian region—when compared to the northeastern region (2.4%, 3/126). This study broadens the herpesvirus host range in cetaceans, including its description in pygmy sperm whales (Kogia breviceps) and humpback whales (Megaptera novaeangliae). Further studies must elucidate herpesvirus drivers in cetaceans.
Accurate estimation of concrete consumption in tunnel lining using terrestrial laser scanning
Authors: Jian Liao , Qiu Wenge , Cheng Yunjian
Published: February 2024

Accurate estimation of concrete (including shotcrete) consumption plays a crucial role in tunnel construction. A novel method has been introduced to accurately estimate concrete consumption with terrestrial laser scanning (TLS). The estimation needs to capture TLS data of tunnel surfaces at different stages of construction. Unrolling point clouds, a novel two-stage algorithm consisting of noise removal and hole filling has been used to generate resampled points. Furthermore, resampled points from two scans (before and after lining construction) ultimately generate an innovative computation model composed of multiple hexahedral elements, which is used for calculating volumes. The proposed technique was applied to the Tiantaishan highway tunnel and Da Fang Shan high-speed railway tunnel. The calculation relative error of the rebound rate is 0.19%, and the average relative error in predicting the demand for secondary lining concrete is 0.15%. Compared with 3D Delaunay with curve fitting, the proposed technique offers a more straightforward operation and higher accuracy. Considering factors such as tunnel geometry, support design, and concrete properties, a computational model will provide valuable insights into optimizing resource allocation and reducing material waste during construction.
Exploration of nonlinear optical properties of 4-methyl-4H-1,2,4-triazol-3-yl)thio)-N-phenylpropanamide based derivatives: experimental and DFT approach
Authors: Irfan Muhammad , Khan Hammad Ali , Bibi Shamsa , Wu Gang , Ali Akbar , Khan Samreen Gul , Alhokbany Norah , Rasool Faiz , Chen Ke
Published: February 2024

Triazoles, nitrogen-containing heterocycles, have gained attention for their applications in medicinal chemistry, drug discovery, agrochemicals, and material sciences. In the current study, we synthesized novel derivatives of N-substituted 2-((5-(3-bromophenyl)-4-methyl-4H-1,2,4-triazol-3-yl)thio)-N-phenylpropanamide and conducted a comprehensive investigation using density functional theory (DFT). These novel structural hybrids of 1,2,4-triazole were synthesized through the multi-step chemical modifications of 3-bromobenzoic acid (1). Initially, compound 1 was converted into its methyl-3-bromobenzoate (2) which was then transformed into 3-bromobenzohydrazide (3). The final step involved the cyclization of compound 3, producing its 1,2,4-triazole derivative (4). This intermediate was then coupled with different electrophiles, resulting in the formation of the final derivatives (7a–7c). Additionally, the characterization of these triazole-based compounds (7a, 7b, and 7c) were carried out using techniques such as IR, HNMR, and UV–visible spectroscopy to understand their structural and spectroscopic properties. The DFT study utilized M06/6-311G(d,p) functional to investigate geometrical parameters, HOMO–LUMO energies, natural bond orbital analyses, transition density matrix (TDM), density of states, and nonlinear optical (NLO) properties. The FMO analysis revealed that compound 7c exhibited the lowest band gap value (4.618 eV). Notably, compound 7c exhibited significant linear polarizability (4.195 >  × 10–23) and first and second hyperpolarizabilities (6.317 >  × 10–30, 4.314 × 10–35), signifying its potential for nonlinear optical applications. These NLO characteristics imply that each of our compounds, especially 7c, plays a crucial part in fabricating materials showing promising NLO properties for optoelectronic applications.
Near-infrared spectroscopy for kidney oxygen monitoring in a porcine model of hemorrhagic shock, hemodilution, and REBOA
Authors: Silverton Natalie A. , Lofgren Lars R. , Kuck Kai , Stoddard Gregory J. , Johnson Russel , Ramezani Ali , Hoareau Guillaume L.
Published: February 2024

Acute kidney injury is a common complication of trauma and hemorrhagic shock. In a porcine model of hemorrhagic shock, resuscitative endovascular balloon aortic occlusion (REBOA) and hemodilution, we hypothesized that invasive kidney oxygen concentration measurements would correlate more strongly with noninvasive near infra-red spectroscopy (NIRS) oxygen saturation measurements when cutaneous sensors were placed over the kidney under ultrasound guidance compared to placement over the thigh muscle and subcutaneous tissue. Eight anesthetized swine underwent hemorrhagic shock 4 of which were resuscitated with intravenous fluids prior to the return of shed blood (Hemodilution protocol) and 4 of which underwent REBOA prior to resuscitation and return of shed blood (REBOA protocol). There was a moderate correlation between the NIRS and kidney tissue oxygen measurements (r = 0.61 p < 0.001; r = 0.67 p < 0.001; r = 0.66 p < 0.001for left kidney, right kidney, and thigh NIRS respectively). When the animals were separated by protocol, the Hemodilution group showed a weak or nonsignificant correlation between NIRS and kidney tissue oxygen measurements (r = 0.10 p < 0.001; r = 0.01 p = 0.1007; r = 0.28 p < 0.001 for left kidney, right kidney, and thigh NIRS respectively). This contrasts with the REBOA group, where left and right kidney as well as thigh NIRS were moderately correlated with kidney tissue oxygen (r = 0.71 p < 0.001; r = 0.74 p < 0.001; r = 0.70 p < 0.001; for left kidney, right kidney, and thigh NIRS respectively). There was a strong correlation between both kidney NIRS signals and thigh NIRS measurements (r = 0.85 p < 0.001; r = 0.88 p < 0.001;for left kidney vs thigh and right kidney vs thigh respectively). There was also a strong correlation between left and right kidney NIRS (r = 0.90 p < 0.001). These relationships were maintained regardless of the resuscitation protocol. These results suggest that kidney NIRS measurements were more closely related to thigh NIRS measurements than invasive kidney tissue oxygen concentration.
Exploring the links of skeletal muscle mitochondrial oxidative capacity, physical functionality, and mental well-being of cancer survivors
Authors: Gonsalves Stephen G. , Saligan Leorey N. , Bergeron Christopher M. , Lee Philip R. , Fishbein Kenneth W. , Spencer Richard G. , Zampino Marta , Sun Xinyi , Sheng Jennifer Yeong-Shin , Stearns Vered , Carducci Michael , Ferrucci Luigi , Lukkahatai Nada
Published: February 2024

Physical impairments following cancer treatment have been linked with the toxic effects of these treatments on muscle mass and strength, through their deleterious effects on skeletal muscle mitochondrial oxidative capacity. Accordingly, we designed the present study to explore relationships of skeletal muscle mitochondrial oxidative capacity with physical performance and perceived cancer-related psychosocial experiences of cancer survivors. We assessed skeletal muscle mitochondrial oxidative capacity using in vivo phosphorus-31 magnetic resonance spectroscopy (31P MRS), measuring the postexercise phosphocreatine resynthesis time constant, τPCr, in 11 post-chemotherapy participants aged 34–70 years. During the MRS procedure, participants performed rapid ballistic knee extension exercise to deplete phosphocreatine (PCr); hence, measuring the primary study outcome, which was the recovery rate of PCr (τPCr). Patient-reported outcomes of psychosocial symptoms and well-being were assessed using the Patient-Reported Outcomes Measurement Information System and the 36-Item Short Form health survey (SF-36). Rapid bioenergetic recovery, reflected through a smaller value of τPCr was associated with worse depression (rho ρ = − 0.69, p = 0.018, and Cohen’s d = − 1.104), anxiety (ρ = − 0.61, p = .046, d = − 0.677), and overall mental health (ρ = 0.74, p = 0.010, d = 2.198) scores, but better resilience (ρ = 0.65, p = 0.029), and coping-self efficacy (ρ = 0.63, p = 0.04) scores. This is the first study to link skeletal muscle mitochondrial oxidative capacity with subjective reports of cancer-related behavioral toxicities. Further investigations are warranted to confirm these findings probing into the role of disease status and personal attributes in these preliminary results.
Generation WhatsApp: inter-brain synchrony during face-to-face and texting communication
Authors: Schwartz Linoy , Levy Jonathan , Hayut Olga , Netzer Ofir , Endevelt-Shapira Yaara , Feldman Ruth
Published: February 2024

Texting has become one of the most prevalent ways to interact socially, particularly among youth; however, the effects of text messaging on social brain functioning are unknown. Guided by the biobehavioral synchrony frame, this pre-registered study utilized hyperscanning EEG to evaluate interbrain synchrony during face-to-face versus texting interactions. Participants included 65 mother-adolescent dyads observed during face-to-face conversation compared to texting from different rooms. Results indicate that both face-to-face and texting communication elicit significant neural synchrony compared to surrogate data, demonstrating for the first time brain-to-brain synchrony during texting. Direct comparison between the two interactions highlighted 8 fronto-temporal interbrain links that were significantly stronger in the face-to-face interaction compared to texting. Our findings suggest that partners co-create a fronto-temporal network of inter-brain connections during live social exchanges. The degree of improvement in the partners' right-frontal-right-frontal connectivity from texting to the live social interaction correlated with greater behavioral synchrony, suggesting that this well-researched neural connection may be specific to face-to-face communication. Our findings suggest that while technology-based communication allows humans to synchronize from afar, face-to-face interactions remain the superior mode of communication for interpersonal connection. We conclude by discussing the potential benefits and drawbacks of the pervasive use of texting, particularly among youth.
Fingertip tactile sensation via piezoelectric micromachined ultrasonic transducers with an amplified interface
Authors: Sone Junji
Published: February 2024

Tactile devices are often used in the field of robotics; however, the development of compact high-resolution tactile devices remains challenging. In this study, we developed a haptic device for force presentation using a DC motor and a tactile sensation device to simultaneously present haptic and tactile stimuli. A microelectromechanical system was selected to maintain the compactness of the tactile device. Piezoelectric micromachined ultrasonic transducers are known for high-power stimulation, and we selected lanthanum-doped lead zirconate titanate as the high-power amplified actuator. A finger mount structure that transfers force for amplifying ultrasonic waves was considered to combine acoustic pressure and aeroacoustics by attaching silicone rubber. The device was fabricated, and the performance of the tactile sensations was evaluated. The developed device uses the novel concept of combining acoustic pressure and aeroacoustics, and its compactness renders it suitable for wearable systems.
Comparing domain- and intensity-specific physical activity in coronary heart disease and non-CHD individuals
Authors: Goo Seon Young , Lee Mi Kyung , Lee Dong Hoon , Park Dong - Hyuk , Lee Tae Ho , Lee Chan Joo , Lee Jong-Young , Jeon Justin Y.
Published: February 2024

Although increase in physical activity is important to improve prognosis of cardiac patients in addition to hospital-based exercise cardiac rehabilitation, their physical activity levels are not properly understood. This study aimed to examine domain- and intensity-specific physical activity in individuals with coronary heart disease (CHD) and compare them with non-CHD individuals. Data from the Korean National Health and Nutrition Examination Survey (KNHANES) from 2014 to 2019 were analyzed, including 1083 CHD patients and 38,532 non-CHD individuals. The inclusion criteria were age 19 years or older and data not missing for CHD information. Before and after propensity score matching (PSM) for age, sex, body mass index, education, household income, alcohol intake, and smoking status, domain (leisure, work, transportation)—and intensity (moderate, vigorous) -specific physical activity participation levels were compared between individuals with and without CHD. Before PSM, CHD individuals were older, less educated, more sedentary, and participated less in PAs compared to non-CHD individuals. After PSM, CHD individuals had similar levels of domain-specific PAs. However, they had higher work-related PA levels (29.7 ± 209.6 vs. 42.1 ± 291.3 min/week p = 0.022) and more sedentary time (487.2 ± 224.2 vs. 514.1 ± 228.7. p = 0.003) than those without CHD. Subgroup analysis revealed lower leisure-related PA in men with CHD (63.5 ± 165.5 vs. 47.3 ± 140.2, p < 0.05) and higher work-related PA in women with CHD (18.9 ± 159.7 vs. 57.1 ± 397.5, p < 0.01). Among those < 65 years of age, individuals with CHD spent more time sedentary than individuals without CHD. CHD individuals are not physically inactive compared with non-CHD individuals who are similar in sociodemographic status and lifestyle. CHD patients’ PA levels may have been underestimated.
Detection of novel drug-adverse drug reaction signals in rheumatoid arthritis and ankylosing spondylitis: analysis of Korean real-world biologics registry data
Authors: Kwon M. , Joung C. I. , Shin H. , Lee C. C. , Song Y. S. , Lee Y. J. , Kang S. , Kim J. Y. , Lee S.
Published: February 2024

This study aimed to detect signals of adverse drug reactions (ADRs) associated with biological disease-modifying antirheumatic drugs (DMARDs) and targeted therapies in rheumatoid arthritis (RA) and ankylosing spondylitis (AS) patients. Utilizing the KOrean College of Rheumatology BIOlogics & Targeted Therapy Registry (KOBIO) data, we calculated relative risks, excluded previously reported drug-ADR pairs, and externally validated remaining pairs using US Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) and single centre’s electronic health records (EHR) data. Analyzing data from 2279 RA and 1940 AS patients, we identified 35 significant drug-ADR pairs in RA and 26 in AS, previously unreported in drug labels. Among the novel drug-ADR pairs from KOBIO, 15 were also significant in the FAERS data. Additionally, 2 significant drug-laboratory abnormality pairs were found in RA using CDM MetaLAB analysis. Our findings contribute to the identification of 14 novel drug-ADR signals, expanding our understanding of potential adverse effects related to biological DMARDs and targeted therapies in RA and AS. These results emphasize the importance of ongoing pharmacovigilance for patient safety and optimal therapeutic interventions.
Droplet impact on doubly re-entrant structures
Authors: Singh Navdeep Sangeet , Jitniyom Thanaphun , Navarro-Cía Miguel , Gao Nan
Published: February 2024

Doubly re-entrant pillars have been demonstrated to possess superior static and dynamic liquid repellency against highly wettable liquids compared to straight or re-entrant pillars. Nevertheless, there has been little insight into how the key structural parameters of doubly re-entrant pillars influence the hydrodynamics of impacting droplets. In this work, we carried out numerical simulations and experimental studies to portray the fundamental physical phenomena that can explain the alteration of the surface wettability from adjusting the design parameters of the doubly re-entrant pillars. On the one hand, three-dimensional multiphase flow simulations of droplet impact were conducted to probe the predominance of the overhang structure in dynamic liquid repellency. On the other hand, the numerical results of droplet impact behaviours are agreed by the experimental results for different pitch sizes and contact angles. Furthermore, the dimensions of the doubly re-entrant pillars, including the height, diameter, overhang length and overhang thickness, were altered to establish their effect on droplet repellency. These findings present the opportunity for manipulations of droplet behaviours by means of improving the critical dimensional parameters of doubly re-entrant structures.
Improving the quality properties of soybean oil by using rice bran oil
Authors: Mohammed Ahmed Sabry , Hashem Hanafy Abdel Aziz , Abdel Maksoud Badr Saed
Published: February 2024

This study aims to study the effect of substituting soybean oil (SO) with rice bran oil (RBO) at different levels (25%, 50%, and 75%) on the physical and chemical properties, fatty acid composition, and oxidative stability of SO, also, study the effect of storing SO, RBO, and their blend oils at ambient temperature for a period of 12 months on the content of free fatty acids (% FFA), peroxide value (PV), and thiobarbituric acid (TBA). RBO demonstrated good quality, as evidenced by its initial low values of % FFA, PV, and TBA. Furthermore, RBO was found to be an excellent source of γ-oryzanol, whereas the other oils lacked this compound. Consequently, increasing the proportion of RBO in SO resulted in the least degradation, while pure SO exhibited the highest degree of degradation. Moreover, the blend oils demonstrated an inhibitory effect against oxidation, allowing for a prolonged storage period without the use of industrial antioxidants. Throughout the entire storage period, the % FFA and PV of all tested blend oil samples remained within the limits recommended for human consumption. TBA exhibited a similar trend to PV. However, an incremental increase in TBA values was observed as the storage period of the oils extended. In SO, TBA levels increased from 0.533 mg malonaldehyde/kg oil at the beginning to 1.446 mg malonaldehyde/kg oil after 12 months of storage. In RBO, TBA levels increased from 0.336 mg malonaldehyde/kg oil at the beginning to 0.882 mg malonaldehyde/kg oil after 12 months of storage.
Wireless body area sensor networks based human activity recognition using deep learning
Authors: El-Adawi Ehab , Essa Ehab , Handosa Mohamed , Elmougy Samir
Published: February 2024

In the healthcare sector, the health status and biological, and physical activity of the patient are monitored among different sensors that collect the required information about these activities using Wireless body area network (WBAN) architecture. Sensor-based human activity recognition (HAR), which offers remarkable qualities of ease and privacy, has drawn increasing attention from researchers with the growth of the Internet of Things (IoT) and wearable technology. Deep learning has the ability to extract high-dimensional information automatically, making end-to-end learning. The most significant obstacles to computer vision, particularly convolutional neural networks (CNNs), are the effect of the environment background, camera shielding, and other variables. This paper aims to propose and develop a new HAR system in WBAN dependence on the Gramian angular field (GAF) and DenseNet. Once the necessary signals are obtained, the input signals undergo pre-processing through artifact removal and median filtering. In the initial stage, the time series data captured by the sensors undergoes a conversion process, transforming it into 2-dimensional images by using the GAF algorithm. Then, DenseNet automatically makes the processes and integrates the data collected from diverse sensors. The experiment results show that the proposed method achieves the best outcomes in which it achieves 97.83% accuracy, 97.83% F-measure, and 97.64 Matthews correlation coefficient (MCC).
Calcium carbonate precipitating extremophilic bacteria in an Alpine ice cave
Authors: Lange-Enyedi Nóra Tünde , Németh Péter , Borsodi Andrea K. , Spötl Christoph , Makk Judit
Published: February 2024

Extensive research has provided a wealth of data on prokaryotes in caves and their role in biogeochemical cycles. Ice caves in carbonate rocks, however, remain enigmatic environments with limited knowledge of their microbial taxonomic composition. In this study, bacterial and archaeal communities of the Obstans Ice Cave (Carnic Alps, Southern Austria) were analyzed by next-generation amplicon sequencing and by cultivation of bacterial strains at 10 °C and studying their metabolism. The most abundant bacterial taxa were uncultured Burkholderiaceae and Brevundimonas spp. in the drip water, Flavobacterium, Alkanindiges and Polaromonas spp. in the ice, Pseudonocardia, Blastocatella spp., uncultured Pyrinomonadaceae and Sphingomonadaceae in carbonate precipitates, and uncultured Gemmatimonadaceae and Longimicrobiaceae in clastic cave sediments. These taxa are psychrotolerant/psychrophilic and chemoorganotrophic bacteria. On a medium with Mg2+/Ca2+ = 1 at 21 °C and 10 °C, 65% and 35% of the cultivated strains precipitated carbonates, respectively. The first ~ 200 µm-size crystals appeared 2 and 6 weeks after the start of the cultivation experiments at 21 °C and 10 °C, respectively. The crystal structure of these microbially induced carbonate precipitates and their Mg-content are strongly influenced by the Mg2+/Ca2+ ratio of the culture medium. These results suggest that the high diversity of prokaryotic communities detected in cryogenic subsurface environments actively contributes to carbonate precipitation, despite living at the physical limit of the presence of liquid water.