bluuebunny/crossref_metadata_2025 huggingface dataset:
On-Body Edge Computing Through E-Textile Programmable Logic Array
Authors: Cleary Frances , Henshall David C. , Balasubramaniam Sasitharan
Published: June 2021

E-textiles have received tremendous attention in recent years due to the capability of integrating sensors into a garment, enabling high-precision sensing of the human body. Besides sensing, a number of solutions for e-textile garments have also integrated wireless interfaces, allowing sensing data to be transmitted, and also inbuilt capacitive touch sensors, allowing users to provide instructions. While this has provided a new level of sensing that can result in unprecedented applications, there has been little attention placed around on-body edge computing for e-textiles. This study focuses on the need for a noninvasive and remote health-monitoring solution with inbuilt on-body edge computing, and how enabling such sensing and computing capabilities in a fabric environment can act as a new method for healthcare monitoring through the use of embedded computing intelligence in smart garments. Facilitating computing in e-textiles can result in a new form of on-body edge computing, where sensor information is processed very close to the body before being transmitted to an external device or wireless access point. This form of computing can provide new security and data privacy capabilities and, at the same time, provide opportunities for new energy-harvesting mechanisms to process the data through the garment. This study proposes this concept through embroidered programmable logic arrays (PLAs) integrated into e-textiles. In the same way that PLAs have programmable logic circuits by interconnecting different AND, NOT, and OR gates, we propose e-textile–based gates that are sewn into a garment and connected through conductive thread stitching. Two designs are proposed, and this includes single- and multi-layered PLAs. Experimental validations have been conducted at the individual gates and the entire PLA circuits to determine the voltage utilization and logic computing reliability. The multilayered PLA garment superseded the single-layered garment with higher levels of accuracy in the yielded results due to the enhanced design layout, which reduces the potential for short circuits and errors occurring. Our proposed approach can usher in a new form of on-body edge computing for e-textile garments for future wearable technologies, and, in particular, with the current pandemic that requires noninvasive remote health-monitoring applications.
Model-Augmented Haptic Telemanipulation: Concept, Retrospective Overview, and Current Use Cases
Authors: Hulin Thomas , Panzirsch Michael , Singh Harsimran , Coelho Andre , Balachandran Ribin , Pereira Aaron , Weber Bernhard M. , Bechtel Nicolai , Riecke Cornelia , Brunner Bernhard , Lii Neal Y. , Klodmann Julian , Hellings Anja , Hagmann Katharina , Quere Gabriel , Bauer Adrian S. , Sierotowicz Marek , Lampariello Roberto , Vogel Jörn , Dietrich Alexander , Leidner Daniel , Ott Christian , Hirzinger Gerd , Albu-Schäffer Alin
Published: June 2021

Certain telerobotic applications, including telerobotics in space, pose particularly demanding challenges to both technology and humans. Traditional bilateral telemanipulation approaches often cannot be used in such applications due to technical and physical limitations such as long and varying delays, packet loss, and limited bandwidth, as well as high reliability, precision, and task duration requirements. In order to close this gap, we research model-augmented haptic telemanipulation (MATM) that uses two kinds of models: a remote model that enables shared autonomous functionality of the teleoperated robot, and a local model that aims to generate assistive augmented haptic feedback for the human operator. Several technological methods that form the backbone of the MATM approach have already been successfully demonstrated in accomplished telerobotic space missions. On this basis, we have applied our approach in more recent research to applications in the fields of orbital robotics, telesurgery, caregiving, and telenavigation. In the course of this work, we have advanced specific aspects of the approach that were of particular importance for each respective application, especially shared autonomy, and haptic augmentation. This overview paper discusses the MATM approach in detail, presents the latest research results of the various technologies encompassed within this approach, provides a retrospective of DLR's telerobotic space missions, demonstrates the broad application potential of MATM based on the aforementioned use cases, and outlines lessons learned and open challenges.
A Neurosociological Theory of Culturally and Structurally Situated Cognition and Ethno-Racial Stress
Authors: Firat Rengin B.
Published: June 2021

A longstanding body of literature reveals that experiences of discrimination and exclusion lead to health disadvantages by increasing physiological stress responses both in the body and the brain. However, a sociological view that takes into account structurally and culturally shaped biological processes is missing from the literature. Building on recent literature from the sociology of morality and values and the dual process model of culture, this paper proposes and provides preliminary evidence for an applied theory of culturally situated moral cognition as a coping mechanism with ethno-racial stress. I focus on values as they help cope with ethnicity and race related stress such as discrimination. Using functional neuroimaging data, I offer evidence that values operate through both explicit (controlled and conscious) processes recruiting brain regions like the dorsal prefrontal cortex, and implicit (automatic and non-conscious) processes recruiting regions like the ventromedial prefrontal cortex, to help cope with exclusion and discrimination.
Effect of Salinity Stress on Physiological Changes in Winter and Spring Wheat
Authors: Saddiq Muhammad Sohail , Iqbal Shahid , Hafeez Muhammad Bilal , Ibrahim Amir M. H. , Raza Ali , Fatima Esha Mehik , Baloch Heer , Jahanzaib  , Woodrow Pasqualina , Ciarmiello Loredana Filomena
Published: June 2021

Salinity is a leading threat to crop growth throughout the world. Salt stress induces altered physiological processes and several inhibitory effects on the growth of cereals, including wheat (Triticum aestivum L.). In this study, we determined the effects of salinity on five spring and five winter wheat genotypes seedlings. We evaluated the salt stress on root and shoot growth attributes, i.e., root length (RL), shoot length (SL), the relative growth rate of root length (RGR-RL), and shoot length (RGR-SL). The ionic content of the leaves was also measured. Physiological traits were also assessed, including stomatal conductance (gs), chlorophyll content index (CCI), and light-adapted leaf chlorophyll fluorescence, i.e., the quantum yield of photosystem II (Fv′/Fm′) and instantaneous chlorophyll fluorescence (Ft). Physiological and growth performance under salt stress (0, 100, and 200 mol/L) were explored at the seedling stage. The analysis showed that spring wheat accumulated low Na+ and high K+ in leaf blades compared with winter wheat. Among the genotypes, Sakha 8, S-24, W4909, and W4910 performed better and had improved physiological attributes (gs, Fv′/Fm′, and Ft) and seedling growth traits (RL, SL, RGR-SL, and RGR-RL), which were strongly linked with proper Na+ and K+ discrimination in leaves and the CCI in leaves. The identified genotypes could represent valuable resources for genetic improvement programs to provide a greater understanding of plant tolerance to salt stress.
Intercropping Alfalfa into Silage Maize Can Be More Profitable Than Maize Silage Followed by Spring-Seeded Alfalfa
Authors: Berti Marisol T. , Lukaschewsky Johanna , Samarappuli Dulan P.
Published: June 2021

Intercropping of silage maize (Zea mays L.) and alfalfa (Medicago sativa L.) is not a common practice because alfalfa generally reduces maize grain and biomass yield. The objective of this research was to evaluate the productivity and profitability of silage maize–alfalfa intercropping, with a goal to establish alfalfa and increase alfalfa productivity in the first year of production. The experiment was conducted in Fargo and Prosper, ND, USA, in 2014–2017. The design was a randomized complete block with four replicates and a split-plot arrangement. The main plot had two maize row-spacing treatments (RS), 61 and 76 cm, respectively. Treatments in the subplot were: (1) maize monoculture, (2) maize intercropped with alfalfa, (3) maize intercropped with alfalfa + prohexadione-calcium (PHX), and (4) spring-seeded alfalfa in the following year (simulating a maize–spring-seeded alfalfa crop sequence). Both alfalfa and maize were seeded the same day in May of 2014 at both locations. Prohexadione-calcium, a growth regulator to reduce internode length and avoid etiolation of alfalfa seedlings, did not improve alfalfa plant survival. Averaged across locations, RS did not have an effect on silage maize yield and alfalfa forage yield. Alfalfa established in intercropping with maize had almost double the forage yield in the following year compared with spring-seeded alfalfa following a crop of silage maize. Considering a two-year system, alfalfa intercropped with maize had higher net returns than a silage-maize followed by a spring-seeded alfalfa sequence. This system has the potential to get more growers to have alfalfa in the rotation skipping the typical low forage yield of alfalfa in the establishment year.
Live Cell Assays for the Assessment of Antioxidant Activities of Plant Extracts
Authors: Furger Christophe
Published: June 2021

Plant extracts and pharmacopoeias represent an exceptional breeding ground for the discovery of new antioxidants. Until recently, the antioxidant activity was only measured by chemical hydrogen atom transfer (HAT) and single-electron transfer (SET) cell-free assays that do not inform about the actual effect of antioxidants in living systems. By providing information about the mode of action of antioxidants at the subcellular level, recently developed live cell assays are now changing the game. The idea of this review is to present the different cell-based approaches allowing a quantitative measurement of antioxidant effects of plant extracts. Up to date, only four different approaches have reached a certain degree of standardization: (1) the catalase-like assay using H2O2 as a stressor, (2) the cell antioxidant assay (CAA) using AAPH as a stressor and DCFH-DA as a readout, (3) the AOP1 assay which uses photoinduction to monitor and control cell ROS production, and (4) the Nrf2/ARE gene reporter system. The molecular aspects of these assays are presented in detail along with their features, drawbacks, and benefits. The Nrf2/ARE gene reporter system dedicated to indirect antioxidant effect measurement currently represents the most standardized approach with high-throughput applications. AOP1, the first technology linking a fine-tuning of cell ROS production with a quantitative signal, appears to be the most promising tool for the assessment of direct cellular ROS-scavenging effects at an industrial scale.
Low Preconception Complement Levels Are Associated with Adverse Pregnancy Outcomes in a Multicenter Study of 260 Pregnancies in 197 Women with Antiphospholipid Syndrome or Carriers of Antiphospholipid Antibodies
Authors: Nalli Cecilia , Lini Daniele , Andreoli Laura , Crisafulli Francesca , Fredi Micaela , Lazzaroni Maria Grazia , Bitsadze Viktoria , Calligaro Antonia , Canti Valentina , Caporali Roberto , Carubbi Francesco , Chighizola Cecilia Beatrice , Conigliaro Paola , Conti Fabrizio , De Carolis Caterina , Del Ross Teresa , Favaro Maria , Gerosa Maria , Iuliano Annamaria , Khizroeva Jamilya , Makatsariya Alexander , Meroni Pier Luigi , Mosca Marta , Padovan Melissa , Perricone Roberto , Rovere-Querini Patrizia , Sebastiani Gian Domenico , Tani Chiara , Tonello Marta , Truglia Simona , Zucchi Dina , Franceschini Franco , Tincani Angela
Published: June 2021

Antiphospholipid antibodies (aPL) can induce fetal loss in experimental animal models. Human studies did find hypocomplementemia associated with pregnancy complications in patients with antiphospholipid syndrome (APS), but these results are not unanimously confirmed. To investigate if the detection of low C3/C4 could be considered a risk factor for adverse pregnancy outcomes (APO) in APS and aPL carriers’ pregnancies we performed a multicenter study including 503 pregnancies from 11 Italian and 1 Russian centers. Data in women with APS and asymptomatic carriers with persistently positive aPL and preconception complement levels were available for 260 pregnancies. In pregnancies with low preconception C3/C4, a significantly higher prevalence of pregnancy losses was observed (p = 0.008). A subgroup analysis focusing on triple aPL-positive patients found that preconception low C3 and/or C4 levels were associated with an increased rate of pregnancy loss (p = 0.05). Our findings confirm that decreased complement levels before pregnancy are associated with increased risk of APO. This has been seen only in women with triple aPL positivity, indeed single or double positivity does not show this trend. Complement levels are cheap and easy to be measured therefore they could represent a useful aid to identify patients at increased risk of pregnancy loss.
MITF Promotes Cell Growth, Migration and Invasion in Clear Cell Renal Cell Carcinoma by Activating the RhoA/YAP Signal Pathway
Authors: Kim Nayoung , Kim Solbi , Lee Myung-Won , Jeon Heung-Jin , Ryu Hyewon , Kim Jin-Man , Lee Hyo-Jin
Published: June 2021

Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor involved in the lineage-specific regulation of melanocytes, osteoclasts and mast cells. MITF is also involved in the progression of melanomas and other carcinomas, including the liver, pancreas and lung. However, the role of MITF in clear cell renal cell carcinoma (ccRCC) is largely unknown. This study investigates the functional role of MITF in cancer and the molecular mechanism underlying disease progression in ccRCC. MITF knockdown inhibited cell proliferation and shifted the cell cycle in ccRCC cells. In addition, MITF knockdown reduced wound healing, cell migration and invasion compared with the controls. Conversely, MITF overexpression in SN12C and SNU482 cells increased cell migration and invasion. Overexpression of MITF activated the RhoA/YAP signaling pathway, which regulates cell proliferation and invasion, and increased YAP signaling promoted cell cycle-related protein expression. Additionally, tumor formation was impaired by MITF knockdown and enhanced by MITF overexpression in vivo. In summary, MITF expression was associated with aggressive tumor behavior, and increased the migratory and invasive capabilities of ccRCC cells. These effects were reversed by MITF suppression. These results suggest that MITF is a potential therapeutic target for the treatment of ccRCC.
The Human TOR Signaling Regulator Is the Key Indicator of Liver Cancer Patients’ Overall Survival: TIPRL/LC3/CD133/CD44 as Potential Biomarkers for Early Liver Cancers
Authors: Jun Soo Young , Yoon Hyang Ran , Yoon Ji-Yong , Jeon Su-Jin , Lee Jeong-Ju , Halder Debasish , Kim Jin-Man , Kim Nam-Soon
Published: June 2021

Recently, we reported the involvement of TIPRL/LC3/CD133 in liver cancer aggressiveness. This study assessed the human TOR signaling regulator (TIPRL)/microtubule-associated light chain 3 (LC3)/prominin-1 (CD133)/cluster of differentiation 44 (CD44) as potential diagnostic and prognostic biomarkers for early liver cancer. For the assessment, we stained tissues of human liver disease/cancer with antibodies against TIPRL/LC3/CD133/CD44/CD46, followed by confocal observation. The roles of TIPRL/LC3/CD133/CD44/CD46 in liver normal and cancer cell lines were determined by in vitro studies. We analyzed the prognostic and diagnostic potentials of TIPRL/LC3/CD133/CD44/CD46 using the receiver-operating characteristic curve, a Kaplan–Meier and uni-/multi-Cox analyses. TIPRL and LC3 were upregulated in tissues of HCCs and adult hepatocytes-derived liver diseases while downregulated in iCCA. Intriguingly, TIPRL levels were found to be critically associated with liver cancer patients’ survivability, and TIPRL is the key player in liver cancer cell proliferation and viability via stemness and self-renewal induction. Furthermore, we demonstrate that TIPRL/LC3/CD133 have shown prominent efficiency for diagnosing patients with grade 1 iCCA. TIPRL/LC3/CD133/CD44 have also provided excellent potential for prognosticating patients with grade 1 iCCA and grade 1 HCCs, together with demonstrating that TIPRL/LC3/CD133/CD44 are, either individually or in conjunction, potential biomarkers for early liver cancer.
Evaluation of Darolutamide (ODM201) Efficiency on Androgen Receptor Mutants Reported to Date in Prostate Cancer Patients
Authors: Lallous Nada , Snow Oliver , Sanchez Christophe , Parra Nuñez Ana Karla , Sun Bei , Hussain Ahmed , Lee Joseph , Morin Helene , Leblanc Eric , Gleave Martin E. , Cherkasov Artem
Published: June 2021

Resistance to drug treatments is common in prostate cancer (PCa), and the gain-of-function mutations in human androgen receptor (AR) represent one of the most dominant drivers of progression to resistance to AR pathway inhibitors (ARPI). Previously, we evaluated the in vitro response of 24 AR mutations, identified in men with castration-resistant PCa, to five AR antagonists. In the current work, we evaluated 44 additional PCa-associated AR mutants, reported in the literature, and thus expanded the study of the effect of darolutamide to a total of 68 AR mutants. Unlike other AR antagonists, we demonstrate that darolutamide exhibits consistent efficiency against all characterized gain-of-function mutations in a full-length AR. Additionally, the response of the AR mutants to clinically used bicalutamide and enzalutamide, as well as to major endogenous steroids (DHT, estradiol, progesterone and hydrocortisone), was also investigated. As genomic profiling of PCa patients becomes increasingly feasible, the developed “AR functional encyclopedia” could provide decision-makers with a tool to guide the treatment choice for PCa patients based on their AR mutation status.
CARTmath—A Mathematical Model of CAR-T Immunotherapy in Preclinical Studies of Hematological Cancers
Authors: Barros Luciana R. C. , Paixão Emanuelle A. , Valli Andrea M. P. , Naozuka Gustavo T. , Fassoni Artur C. , Almeida Regina C.
Published: June 2021

Immunotherapy has gained great momentum with chimeric antigen receptor T cell (CAR-T) therapy, in which patient’s T lymphocytes are genetically manipulated to recognize tumor-specific antigens, increasing tumor elimination efficiency. In recent years, CAR-T cell immunotherapy for hematological malignancies achieved a great response rate in patients and is a very promising therapy for several other malignancies. Each new CAR design requires a preclinical proof-of-concept experiment using immunodeficient mouse models. The absence of a functional immune system in these mice makes them simple and suitable for use as mathematical models. In this work, we develop a three-population mathematical model to describe tumor response to CAR-T cell immunotherapy in immunodeficient mouse models, encompassing interactions between a non-solid tumor and CAR-T cells (effector and long-term memory). We account for several phenomena, such as tumor-induced immunosuppression, memory pool formation, and conversion of memory into effector CAR-T cells in the presence of new tumor cells. Individual donor and tumor specificities are considered uncertainties in the model parameters. Our model is able to reproduce several CAR-T cell immunotherapy scenarios, with different CAR receptors and tumor targets reported in the literature. We found that therapy effectiveness mostly depends on specific parameters such as the differentiation of effector to memory CAR-T cells, CAR-T cytotoxic capacity, tumor growth rate, and tumor-induced immunosuppression. In summary, our model can contribute to reducing and optimizing the number of in vivo experiments with in silico tests to select specific scenarios that could be tested in experimental research. Such an in silico laboratory is an easy-to-run open-source simulator, built on a Shiny R-based platform called CARTmath. It contains the results of this manuscript as examples and documentation. The developed model together with the CARTmath platform have potential use in assessing different CAR-T cell immunotherapy protocols and its associated efficacy, becoming an accessory for in silico trials.
Thermal and Structural Properties of High Density Polyethylene/Carbon Nanotube Nanocomposites: A Comparison Study
Authors: Bozeya Ayat , Makableh Yahia F. , Abu-Zurayk Rund , Khalaf Aya , Al Bawab Abeer
Published: June 2021

The effects of functionalization of carbon nanotubes on the properties of nanocomposite sheets prepared from high-density polyethylene (HDPE) and carbon nanotubes (CNTs) were investigated. Carbon nanotubes were first oxidized, followed by amine group functionalization. The Fourier transform-infrared (FTIR) spectroscopy results confirm the presence of oxygenated and amide groups at the surface of the CNTs after each treatment. The HDPE/CNT nanocomposites sheets were prepared using a melt compounding method. Six types of CNTs were used; pristine Single-walled Carbon nanotubes (SWCNT) and pristine Multi-walled Carbon nanotubes (MWCNT), oxidized (O-SWCNT and O-MWCNT) and amide (Amide-SWCNT and Amide-MWCNT). All prepared nanocomposite sheets were characterized using Thermal gravimetric analysis (TGA), Differential scanning calorimetry (DSC), X-ray diffraction (XRD) and scanning electronic microscope (SEM). TGA results measured increased thermal stability of the polymer with the addition of CNTs, O-MWCNT showed the best enhancement. XRD measurements confirmed that the addition of CNTs did not change the crystal structure of the polymer, although the crystallinity was decreased. The maximum crystallinity decrease resulted from O-SWNTs addition to the polymer matrix. SEM imaging showed that oxidized and functionalized CNTs have more even dispersion in the polymer matrix compared with pristine CNTs.
Addressing Common Misconceptions in Food Allergy: A Review
Authors: Anagnostou Aikaterini
Published: June 2021

Background: Food allergies are common, affecting 1 in 13 school children in the United States and their prevalence is increasing. Many misconceptions exist with regards to food allergy prevention, diagnosis and management. Objective: The main objective of this review is to address misconceptions with regards to food allergies and discuss the optimal, evidence-based approach for patients who carry this diagnosis. Observations: Common misconceptions in terms of food allergy prevention include beliefs that breastfeeding and delayed introduction of allergenic foods prevent the development of food allergies. In terms of diagnosis, statements such as ‘larger skin prick tests or/and higher levels of food-specific IgE can predict the severity of food-induced allergic reactions’, or ‘Tryptase is always elevated in food-induced anaphylaxis’ are inaccurate. Additionally, egg allergy is not a contraindication for receiving the influenza vaccine, food-allergy related fatalities are rare and peanut oral immunotherapy, despite reported benefits, is not a cure for food allergies. Finally, not all infants with eczema will develop food allergies and epinephrine auto-injectors may unfortunately be both unavailable and underused in food-triggered anaphylaxis. Conclusions and relevance: Healthcare professionals must be familiar with recent evidence in the food allergy field and avoid common misunderstandings that may negatively affect prevention, diagnosis and management of this chronic disease.
Dual Targeting of Sorafenib-Resistant HCC-Derived Cancer Stem Cells
Authors: Shrestha Ritu , Bridle Kim R. , Cao Lu , Crawford Darrell H. G. , Jayachandran Aparna
Published: June 2021

Sorafenib, an oral multi-tyrosine kinase inhibitor, has been the first-line therapy for the treatment of patients with advanced HCC, providing a survival benefit of only three months in approximately 30% of patients. Cancer stem cells (CSCs) are a rare tumour subpopulation with self-renewal and differentiation capabilities, and have been implicated in tumour growth, recurrence and drug resistance. The process of epithelial-to-mesenchymal transition (EMT) contributes to the generation and maintenance of the CSC population, resulting in immune evasion and therapy resistance in several cancers, including HCC. The aim of this study is to target the chemoresistant CSC population in HCC by assessing the effectiveness of a combination treatment approach with Sorafenib, an EMT inhibitor and an immune checkpoint inhibitor (ICI). A stem-cell-conditioned serum-free medium was utilised to enrich the CSC population from the human HCC cell lines Hep3B, PLC/PRF/5 and HepG2. The anchorage independent spheres were characterised for CSC features. The human HCC-derived spheres were assessed for EMT status and expression of immune checkpoint molecules. The effect of combination treatment with SB431542, an EMT inhibitor, and siRNA-mediated knockdown of programmed cell death protein ligand-1 (PD-L1) or CD73 along with Sorafenib on human HCC-derived CSCs was examined with cell viability and apoptosis assays. The three-dimensional spheres enriched from human HCC cell lines demonstrated CSC-like features. The human HCC-derived CSCs also exhibited the EMT phenotype along with the upregulation of immune checkpoint molecules. The combined treatment with SB431542 and siRNA-mediated PD-L1 or CD73 knockdown effectively enhanced the cytotoxicity of Sorafenib against the CSC population compared to Sorafenib alone, as evidenced by the reduced size and proliferation of spheres. Furthermore, the combination treatment of Sorafenib with SB431542 and PD-L1 or CD73 siRNA resulted in an increased proportion of an apoptotic population, as evidenced by flow cytometry analysis. In conclusion, the combined targeting of EMT and immune checkpoint molecules with Sorafenib can effectively target the CSC tumour subpopulation.
Swarm Robotic Interactions in an Open and Cluttered Environment: A Survey
Authors: Vaidis Maxime , Otis Martin J.-D.
Published: June 2021

Recent population migrations have led to numerous accidents and deaths. Little research has been done to help migrants in their journey. For this reason, a literature review of the latest research conducted in previous years is required to identify new research trends in human-swarm interaction. This article presents a review of techniques that can be used in a robots swarm to find, locate, protect and help migrants in hazardous environment such as militarized zone. The paper presents a swarm interaction taxonomy including a detailed study on the control of swarm with and without interaction. As the interaction mainly occurs in cluttered or crowded environment (with obstacles) the paper discussed the algorithms related to navigation that can be included with an interaction strategy. It focused on comparing algorithms and their advantages and disadvantages.
Status Set Sequential Pattern Mining Considering Time Windows and Periodic Analysis of Patterns
Authors: Zhou Shenghan , Liu Houxiang , Chen Bang , Hou Wenkui , Ji Xinpeng , Zhang Yue , Chang Wenbing , Xiao Yiyong
Published: June 2021

The traditional sequential pattern mining method is carried out considering the whole time period and often ignores the sequential patterns that only occur in local time windows, as well as possible periodicity. Therefore, in order to overcome the limitations of traditional methods, this paper proposes status set sequential pattern mining with time windows (SSPMTW). In contrast to traditional methods, the item status is considered, and time windows, minimum confidence, minimum coverage, minimum factor set ratios and other constraints are added to mine more valuable rules in local time windows. The periodicity of these rules is also analyzed. According to the proposed method, this paper improves the Apriori algorithm, proposes the TW-Apriori algorithm, and explains the basic idea of the algorithm. Then, the feasibility, validity and efficiency of the proposed method and algorithm are verified by small-scale and large-scale examples. In a large-scale numerical example solution, the influence of various constraints on the mining results is analyzed. Finally, the solution results of SSPM and SSPMTW are compared and analyzed, and it is suggested that SSPMTW can excavate the laws existing in local time windows and analyze the periodicity of the laws, which solves the problem of SSPM ignoring the laws existing in local time windows and overcomes the limitations of traditional sequential pattern mining algorithms. In addition, the rules mined by SSPMTW reduce the entropy of the system.
Mixed-Stable Models: An Application to High-Frequency Financial Data
Authors: Belovas Igoris , Sakalauskas Leonidas , Starikovičius Vadimas , Sun Edward W.
Published: June 2021

The paper extends the study of applying the mixed-stable models to the analysis of large sets of high-frequency financial data. The empirical data under review are the German DAX stock index yearly log-returns series. Mixed-stable models for 29 DAX companies are constructed employing efficient parallel algorithms for the processing of long-term data series. The adequacy of the modeling is verified with the empirical characteristic function goodness-of-fit test. We propose the smart-Δ method for the calculation of the α-stable probability density function. We study the impact of the accuracy of the computation of the probability density function and the accuracy of ML-optimization on the results of the modeling and processing time. The obtained mixed-stable parameter estimates can be used for the construction of the optimal asset portfolio.
Valorization of Prosopis juliflora Woody Biomass in Northeast Brazilian through Dry Torrefaction
Authors: Carneiro-Junior José Airton de Mattos , de Oliveira Giulyane Felix de , Alves Carine Tondo , Andrade Heloysa Martins Carvalho , Beisl Vieira de Melo Silvio Alexandre Beisl Vieira de , Torres Ednildo Andrade
Published: June 2021

Torrefaction has been investigated to improve the desirable properties of biomass as solid biofuel, usually used in natura as firewood in several countries. This paper has the main objective to present a broad characterization of the biomass Prosopis juliflora (P. juliflora), investigating its potential as a solid biofuel after its torrefaction process. The methodology was based on different procedures. The experimental runs were carried out at 230, 270, and 310 °C for 30 min, using a bench-scale torrefaction apparatus, with an inert atmosphere. In order to investigate the effect of temperature in constant time, torrefaction parameters were calculated, such as mass yield, energy yield, calorific value, base-to-acid ratio (B/A), and the alkaline index (AI). The physicochemical properties of the torrefied samples were determined and thermogravimetric analysis was used to determine the kinetic parameters at four different heating rates of 5, 10, 20, and 30 °C/min. Pyrolysis kinetics was investigated using the Flynn-Wall-Ozawa (FWO) and Kissinger-Akahira-Sunose (KAS) isoconversional methods. Highly thermally stable biofuels were obtained due to the great degradation of hemicellulose and cellulose during torrefaction at higher temperatures. The highest heating value (HHV) of the samples varied between 18.3 and 23.1 MJ/kg, and the energy yield between 81.1 and 96.2%. The results indicate that P. juliflora torrefied becomes a more attractive and competitive solid biofuel alternative in the generation of heat and energy in northeast Brazil.
Metrics for Measuring Sustainable Product Design Concepts
Authors: Han Ji , Jiang Pingfei , Childs Peter
Published: June 2021

Although products can contribute to ecosystems positively, they can cause negative environmental impacts throughout their life cycles, from obtaining raw material, production, and use, to end of life. It is reported that most negative environmental impacts are decided at early design phases, which suggests that the determination of product sustainability should be considered as early as possible, such as during the conceptual design stage, when it is still possible to modify the design concept. However, most of the existing concept evaluation methods or tools are focused on assessing the feasibility or creativity of the concepts generated, lacking the measurements of sustainability of concepts. The paper explores key factors related to sustainable design with regard to environmental impacts, and describes a set of objective measures of sustainable product design concept evaluation, namely, material, production, use, and end of life. The rationales of the four metrics are discussed, with corresponding measurements. A case study is conducted to demonstrate the use and effectiveness of the metrics for evaluating product design concepts. The paper is the first study to explore the measurement of product design sustainability focusing on the conceptual design stage. It can be used as a guideline to measure the level of sustainability of product design concepts to support designers in developing sustainable products. Most significantly, it urges the considerations of sustainability design aspects at early design phases, and also provides a new research direction in concept evaluation regarding sustainability.
A Two-Fluid Model for High-Viscosity Upward Annular Flow in Vertical Pipes
Authors: Ribeiro Joseph X. F. , Liao Ruiquan , Aliyu Aliyu M. , Ahmed Salem K. B. , Baba Yahaya D. , Almabrok Almabrok A. , Archibong-Eso Archibong , Liu Zilong
Published: June 2021

Proper selection and application of interfacial friction factor correlations has a significant impact on prediction of key flow characteristics in gas–liquid two-phase flows. In this study, experimental investigation of gas–liquid flow in a vertical pipeline with internal diameter of 0.060 m is presented. Air and oil (with viscosities ranging from 100–200 mPa s) were used as gas and liquid phases, respectively. Superficial velocities of air ranging from 22.37 to 59.06 m/s and oil ranging from 0.05 to 0.16 m/s were used as a test matrix during the experimental campaign. The influence of estimates obtained from nine interfacial friction factor models on the accuracy of predicting pressure gradient, film thickness and gas void fraction was investigated by utilising a two-fluid model. Results obtained indicate that at liquid viscosity of 100 mPa s, the interfacial friction factor correlation proposed by Belt et al. (2009) performed best for pressure gradient prediction while the Moeck (1970) correlation provided the best prediction of pressure gradient at the liquid viscosity of 200 mPa s. In general, these results indicate that the two-fluid model can accurately predict the flow characteristics for liquid viscosities used in this study when appropriate interfacial friction factor correlations are implemented.
Bio-Crude Production through Recycling of Pretreated Aqueous Phase via Activated Carbon
Authors: Shah Ayaz Ali , Toor Saqib Sohail , Nielsen Asbjørn Haaning , Pedersen Thomas Helmer , Rosendahl Lasse Aistrup
Published: June 2021

The management and optimization of the aqueous phase are the major challenges that hinder the promotion of hydrothermal liquefaction (HTL) technology on a commercial scale. Recently, many studies reported about the accumulation of the N-content in the bio-crude with continuous recycling of the aqueous phase from high protein-containing biomass. In the present study, sewage sludge was processed at 350 °C in an autoclave. The produced aqueous phase was treated with activated carbon, and its subsequent recycling effect on the properties of the bio-crude and aqueous phase was investigated. By contacting the aqueous phase with activated carbon, 38–43% of the total nitrogen was removed from the aqueous phase. After applying the treated aqueous phase recycling, the energy recovery of the bio-crude increased from 50 to 61% after three rounds of recycling. From overall carbon/nitrogen recoveries, 50 to 56% of the carbon was transferred to the bio-crude phase and more than 50% of the nitrogen remained in the aqueous phase. The aqueous phase contained mostly of N&O-heterocyclic compounds, small chain organic acids, and amides. ICP-AES analysis showed that more than 80% of the inorganic elements were concentrated into the solid phase.
Lactobacillus ruminis Alleviates DSS-Induced Colitis by Inflammatory Cytokines and Gut Microbiota Modulation
Authors: Yang Bo , Li Mingjie , Wang Shuo , Ross R. Paul , Stanton Catherine , Zhao Jianxin , Zhang Hao , Chen Wei
Published: June 2021

Lactobacillus ruminis can stimulate the immune response in vitro, but previous studies were only carried out in vitro and the anti-inflammatory effects of L. ruminis needs more in vivo evidences. In this study, the immune regulation and potential mechanisms of L. ruminis was investigated in DSS-induced colitis mice. L. ruminis FXJWS27L3 and L. ruminis FXJSW17L1 relieved the symptoms of colitis, including inhibition of colon shortening and colon tissue damage. L. ruminis FXJWS27L3 significantly reduced the pro-inflammatory cytokines IL-1β, TNF-α, and IL-17, while L. ruminis FXJSW17L1 significantly increased short chain fatty acids in mice feces. Moreover, L. ruminis FXJWS27L3 and L. ruminis FXJSW17L1 treatments significantly increased the gut microbiota diversity and balance the intestine microbiota profiles, which improved the imbalance of intestine microbiota composition to a certain extent. The results showed that L. ruminis can alleviate DSS-induced colitis, which possibly was related to promoting the expression of pro-inflammatory cytokines, up-regulating SCFAs and restoring the imbalance of gut microbiota.
The Effect of Multiple Freeze–Thaw Cycles on the Microstructure and Quality of Trachurus murphyi
Authors: Hu Chunlin , Xie Jing
Published: June 2021

Temperature fluctuation in frozen food storage and distribution is the perpetual and core issue faced by the frozen food industry. Ice recrystallisation induced by temperature fluctuations under cold storage causes microstructural changes in fish products and irreversible damages to cells and tissues, which lower the frozen fish quality in the food chain. This study is intended to explore how repeated freezing–thawing affected the microstructure and quality of Trachurus murphyi during its frozen storage. The results showed the consistency between the increase in ice crystal diameter, volume, and porosity in frozen fish and the increase in centrifugal loss (from 22.4% to 25.69%), cooking loss (from 22.32% to 25.19%), conductivity (from 15.28 Ms/cm to 15.70 Ms/cm), TVB-N (from 16.32 mg N/100 g to 19.94 mg N/100 g), K-value (from 3.73% to 7.07%), and amino acid composition. The muscle structure change observed by Fourier-Transform Infrared spectroscopy (FT-IR) showed that the content of α-helix reduced from 59.05% to 51.83%, while the β-sheet fraction grew from 15.44% to 17.11%, β-turns increased from 5.45% to 7.58%, and random coil from 20.06% to 23.49%. Moreover, muscular structure exhibited varying degrees of deterioration with increasing cycles of freezing and thawing as shown by scanning electron microscopy (SEM). We studied the muscular morphology, which included the measurement of porosities (%) of pore that increased (from 1.4% to 4.3%) and pore distribution, by X-ray computed tomography (uCT). The cycles of the freeze–thaw resulted in structural changes, which seemed to be closely associated with ultimate quality of frozen fish products.
Purification, Characterization, and Self-Assembly of the Polysaccharide from Allium schoenoprasum
Authors: Zhang Fengrui , Zheng Jun , Li Zeyu , Cai Zixuan , Wang Fengqiao , Yang Dong
Published: June 2021

The major polysaccharide component from the stalk of Allium schoenoprasum (AssP) was extracted and purified. Gel filtration chromatography purified AssP exhibited a molecular weight of around 1.7 kDa, which was verified by MALDI-ToF-MS. The monosaccharide analysis revealed its composition as rhamnose: arabinose: galactose: glucose: mannose: fructose with a molar ratio of 0.03:2.46:3.71:3.35:1.00:9.93, respectively. The Congo-red assay indicated that there was no tertiary structure of this polysaccharide, however, it self-assembled into a homogenous nanoparticle with a diameter of ~600 nm as revealed by the dynamic light scattering measurement. The solution behavior of this polysaccharide was simulated. The association of this polysaccharide was both time dependent and concentration dependent. AssP forms spherical particles spontaneously as time passes by, and when the AssP concentration increased, the spherical particles increased their sizes and eventually merged into cylindrical micelles. The diversity of AssP hydrodynamic behavior endowed potential versatility in its future applications.
Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond
Authors: Taroepratjeka Dyah Asri Handayani , Imai Tsuyoshi , Chairattanamanokorn Prapaipid , Reungsang Alissara
Published: June 2021

Extreme halophiles offer the advantage to save on the costs of sterilization and water for biohydrogen production from lignocellulosic waste after the pretreatment process with their ability to withstand extreme salt concentrations. This study identifies the dominant hydrogen-producing genera and species among the acclimatized, extremely halotolerant microbial communities taken from two salt-damaged soil locations in Khon Kaen and one location from the salt evaporation pond in Samut Sakhon, Thailand. The microbial communities’ V3–V4 regions of 16srRNA were analyzed using high-throughput amplicon sequencing. A total of 345 operational taxonomic units were obtained and the high-throughput sequencing confirmed that Firmicutes was the dominant phyla of the three communities. Halanaerobium fermentans and Halanaerobacter lacunarum were the dominant hydrogen-producing species of the communities. Spatial proximity was not found to be a determining factor for similarities between these extremely halophilic microbial communities. Through the study of the microbial communities, strategies can be developed to increase biohydrogen molar yield.
The Association of ATG16L1 Variations with Clinical Phenotypes of Adult-Onset Still’s Disease
Authors: Hung Wei-Ting , Hung Shuen-Iu , Chen Yi-Ming , Hsieh Chia-Wei , Chen Hsin-Hua , Tang Kuo-Tung , Chen Der-Yuan , Lan Tsuo-Hung
Published: June 2021

Adult-onset Still’s disease (AOSD) is a rare autoinflammatory disease, which has elevated autophagosome levels regulated by autophagy-related gene (ATG) expression. We investigated the associations of ATG polymorphisms with AOSD susceptibility, clinical manifestations, and disease course. The six-candidate single-nucleotide polymorphisms (SNPs) involved in autophagy were genotyped using direct sequencing on samples from 129 AOSD patients and 129 healthy participants. The differentially expressed gene products were quantified using PCR and ELISA. Significant linkage disequilibrium was noted in three SNPs of autophagy-related 16-like 1 (ATG16L1) gene (rs10210302, rs2241880, and rs1045100). Although the AA/CC/TT haplotype of ATG16L1 was not associated with the susceptibility of our AOSD patients compared with other haplotypes, those carrying this haplotype had lower mRNA expression levels of LC3-II, reflecting by autophagosome formation (p = 0.026). Patients carrying AA/CC/TT haplotype also have a significantly higher proportion of skin rash and a lower proportion of arthritis compared with other haplotypes. The AA/CC/TT haplotype was significantly associated with systemic pattern (odds ratio, 3.25; 95% confidence interval, 1.15–9.14; p = 0.026). In summary, the AA/CC/TT haplotype encoded lower levels of autophagosome formation and was associated with a higher proportion of skin rash and systemic pattern of AOSD compared with other haplotypes.
Genome Size, Chromosome Number and Morphological Data Reveal Unexpected Infraspecific Variability in Festuca (Poaceae)
Authors: Martínez-Sagarra Gloria , Castro Sílvia , Mota Lucie , Loureiro João , Devesa Juan A.
Published: June 2021

Polyploidy has played an important evolutionary role in the genus Festuca (Poaceae), and several ploidy levels (ranging from 2n = 2x = 14 to 2n = 12x = 84) have been detected to date. This study aimed to estimate the genome size and ploidy level of two subspecies belonging to the F. yvesii polyploid complex by flow cytometry and chromosome counting. The phenotypic variation of the cytotypes was also explored, based on herbarium material. The genome size of F. yvesii subsp. lagascae has been estimated for the first time. Nuclear 2C DNA content of F. yvesii subsp. summilusitana ranged from 21.44 to 31.91 pg, while that of F. yvesii subsp. lagascae was from 13.60 to 22.31 pg. We report the highest ploidy level detected for Festuca (2n = 14x = 98) and previously unknown cytotypes. A positive correlation between holoploid genome size and chromosome number counts shown herein was confirmed. The morphometric approach showed a slight trend towards an increase in the size of some organs consistent with the variation in the ploidy level. Differences in characters were usually significant only among the most extreme cytotypes of each subspecies, but, even in this case, the high overlapping ranges prevent their distinction.
Prevalence and Factors Associated with Psychological Problems of Healthcare Workforce in Vietnam: Findings from COVID-19 Hotspots in the National Second Wave
Authors: Tuan Nguyen Quang , Phuong Nguyen Doan , Co Dao Xuan , Son Do Ngoc , Chinh Luong Quoc , Dung Nguyen Huu , Thach Pham The , Thai Nguyen Quoc , Thu Truong Anh , Tuan Nguyen Anh , San Bui Van , Tung Vu Son , An Ngo Van , Khanh Do Nam , Long Vo Hoang , Tai Nguyen , Muoi To , Vinh Nguyen Dai , Thien Nguyen Trong , Nhan Le Duc , Tuan Nguyen Van
Published: June 2021

Adopting a cross-sectional study design, we aimed to examine the prevalence of psychological problems in different healthcare workers during the COVID-19 pandemic in the hospitals in these COVID-19 hotspots (Da Nang city and Quang Nam province) and to explore the socioeconomic and COVID-19 control-related factors that are associated with various psychological problems. A total of 611 healthcare workers were included in the final analysis from 1 August 2020 to 31 August 2020. The prevalence of anxiety, depression, insomnia, and overall psychological problems was 26.84%, 34.70%, 34.53%, and 46.48%, respectively. The prevalence rates of anxiety were approximately equal amongst the groups of healthcare workers, and moderate-to-severe anxiety was the most common in physicians (11.11%). The prevalence of depression was the highest in nurses (38.65%) and moderate-to-severe depression was mainly found in physicians (11.81%). The prevalence rates of insomnia were 34.03% in physicians, 36.20% in nurses, and 31.21% in technicians; in particular, the rate of moderate-to-severe insomnia was higher in physicians and nurses compared to technicians. The prevalence of overall moderate-to-severe psychological problems was the highest among physicians (14.58%), followed by nurses (12.58%) and technicians (9.22%). Statistically significant associated factors of current psychological problems were the occupations of physicians or nurses, less than 1 year of experience, university education, living with 4–5 people, reporting 1000–5000 m distance between home and workplace, participating in the COVID-19 control for less than 1 week, being under social isolation at home, being affected a lot by the community, reporting inadequate equipment in current workplace conditions, frequently working in the department directly in contact with the COVID-19 patients, and feeling anxious, stressed, or sad about current works. Present findings can provide valuable evidence for the policymakers and managers to adopt supportive, encouraging, motivational, protective, training, and educational interventions into healthcare workforce in other parts of Vietnam.