bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Transcriptomics Reveals the Key lncRNA and mRNA of Sunite Sheep Adrenal Gland Affecting Seasonal Reproduction
Authors: Du Xiaolong , He Xiaoyun , Liu Qiuyue , Di Ran , Liu Qingqing , Chu Mingxing
Published: April 2022

The hypothalamic–pituitary–adrenal (HPA) axis plays an important role in the growth and development of mammals. Recently, lncRNA transcripts have emerged as an area of importance in sheep photoperiod and seasonal estrus studies. This research aims to identify lncRNA and mRNA that are differentially expressed in the sheep adrenal gland in long (LP) or short (SP) photoperiods using transcriptome sequencing and bioinformatics analysis based on the OVX + E2 (Bilateral ovariectomy and estradiol-implanted) model. We found significant differences in the expression of lncRNAs in LP42 (where LP is for 42 days) vs. SP-LP42 (where SP is for 42 days followed by LP for 42 days) (n = 304), SP42 (where SP is for 42 days) vs. SP-LP42 (n = 1,110) and SP42 vs. LP42 (n = 928). Cluster analysis and enrichment analysis identified SP42 vs. LP42 as a comparable group of interest and found the following candidate genes related to reproductive phenotype: FGF16, PLGF, CDKN1A, SEMA7A, EDG1, CACNA1C and ADCY5. FGF16 (Up-regulated lncRNA MSTRG.242136 and MSTRG.236582) is the only up-regulated gene that is closely related to oocyte maturation. However, EDG1 (Down-regulated lncRNA MSTRG.43609) and CACNA1C may be related to precocious puberty in sheep. PLGF (Down-regulated lncRNA MSTRG.146618 and MSTRG.247208) and CDKN1A (Up-regulated lncRNA MSTRG.203610 and MSTRG.129663) are involved in the growth and differentiation of placental and retinal vessels, and SEMA7A (Up-regulated lncRNA MSTRG.250579) is essential for the development of gonadotropin-releasing hormone (GnRH) neurons. These results identify novel candidate genes that may regulate sheep seasonality and may lead to new methods for the management of sheep reproduction. This study provides a basis for further explanation of the basic molecular mechanism of the adrenal gland, but also provides a new idea for a comprehensive understanding of seasonal estrus characteristics in Sunite sheep.
Effects of Fermented Feed on the Growth Performance, Intestinal Function, and Microbiota of Piglets Weaned at Different Age
Authors: Liu Shuai , Xiao Hao , Xiong Yunxia , Chen Jingping , Wu Qiwen , Wen Xiaolu , Jiang Zongyong , Wang Li
Published: April 2022

The beneficial function of fermented feed in livestock industry has been widely investigated. However, little is known about the effects of fermented feed on different weaned-day piglets. This study aimed to investigate the effects of fermented diet on the growth performance, intestinal function, and microbiota of piglets weaned at the age of 21 and 28 days. The results found that weaning on day 21 significantly increased (p< 0.05) average daily gain (ADG), and average daily feed intake (ADFI) (calculated based on wet weight and dry matter), while reduced (p< 0.05) feed to gain ratio (F:G), the activities of trypsin and lipase of jejunum and the villus height of ileum, compared with 28-days weaning. The protein levels of Occludin, Claudin-1, and ZO-1 of ileum in the groups weaning on day 21 were less (p< 0.05) than the groups weaning on day 28. Moreover, dietary supplementation with fermented diet upregulated (p< 0.05) the Occludin, Claudin-1, and ZO-1 proteins of ileum, compared with the groups treated with control diet both weaning on day 21 and 28. In addition, dietary supplementation with fermented diet decreased (p< 0.05) the relative abundance ofClostridia(class) and increased (p< 0.05) theBacteroidia(class) level of cecal microbiota, compared with the groups treated with control diet both weaning on day 21 and 28. However, supplementation with fermented diet did not affect the concentrations of short-chain fatty acids in the cecum (p> 0.05). Therefore, our data suggest that the feed digestibility is improved in piglets weaned at 21 days, but intestinal barrier function is weaker than in piglets weaned at 28 days. However, compared with feeding control diet, supplementation with fermented diet both improved the feed conversion and intestinal barrier function of weaned piglets by modulating intestinal microbiota.
Influence of Diabetes-Induced Glycation and Oxidative Stress on the Human Rotator Cuff
Authors: Yoshikawa Tomoya , Mifune Yutaka , Inui Atsuyuki , Nishimoto Hanako , Yamaura Kohei , Mukohara Shintaro , Shinohara Issei , Kuroda Ryosuke
Published: April 2022

Most shoulder rotator cuff tears (RCTs) are caused by non-traumatic age-related rotator cuff degeneration, of which hyperglycemia is a risk factor due to its glycation reaction and oxidative stress. We aimed to identify the influence of diabetes-induced glycation and oxidative stress in patients with non-traumatic shoulder RCTs. Twenty patients, aged over 50 years, with non-traumatic shoulder RCTs participated in this study. Patients with a history of diabetes mellitus or preoperative HbA1c ≥ 6.5% were assigned to the diabetic group (n = 10), and the rest to the non-diabetic group (n = 10). Cell proliferation; expression of genes related to oxidative stress, glycation reaction, inflammation, and collagen; intracellular reactive oxygen species (ROS) levels; and apoptosis rates were analyzed. The diabetic group had significantly lower cell proliferation than the non-diabetic group. In the diabetic group, the mRNA expression levels of NOX1, NOX4, IL6, RAGE, type III collagen, MMP2, TIMP1, and TIMP2 were significantly higher; type I collagen expression was significantly lower; and the rate of ROS-positive cells and apoptotic cells, as well as the expression of advanced glycation end-products (AGEs) and the receptor for AGEs (RAGE), was significantly higher. In conclusion, hyperglycemia caused by diabetes mellitus increased AGE and RAGE expression, and led to increased NOX expression, ROS production, and apoptosis in the human rotator cuff. This provides scope to find a preventive treatment for non-traumatic RCTs by inhibiting glycation and oxidative stress.
Self or Non-Self? It Is also a Matter of RNA Recognition and Editing by ADAR1
Authors: Tassinari Valentina , Cerboni Cristina , Soriani Alessandra
Published: April 2022

A-to-I editing is a post-transcriptional mechanism affecting coding and non-coding dsRNAs, catalyzed by the adenosine deaminases acting on the RNA (ADAR) family of enzymes. A-to-I modifications of endogenous dsRNA (mainly derived from Alu repetitive elements) prevent their recognition by cellular dsRNA sensors, thus avoiding the induction of antiviral signaling and uncontrolled IFN-I production. This process, mediated by ADAR1 activity, ensures the activation of an innate immune response against foreign (non-self) but not self nucleic acids. As a consequence, ADAR1 mutations or its de-regulated activity promote the development of autoimmune diseases and strongly impact cell growth, also leading to cancer. Moreover, the excessive inflammation promoted by Adar1 ablation also impacts T and B cell maturation, as well as the development of dendritic cell subsets, revealing a new role of ADAR1 in the homeostasis of the immune system.
Therapeutic Potential for Intractable Asthma by Targeting L-Type Amino Acid Transporter 1
Authors: Hayashi Keitaro , Kaminuma Osamu
Published: April 2022

Bronchial asthma is a chronic disease characterized by airway inflammation, obstruction, and hyperresponsiveness. CD4+ T cells, particularly T helper (Th) 2 cells, and their specific cytokines are important mediators in asthma pathogenesis. However, it has been established that Th subsets, other than Th2, as well as various cell types, including innate lymphoid cells (ILCs), significantly contribute to the development of allergic inflammation. These cells require facilitated amino acid uptake to ensure their full function upon activation. Emerging studies have suggested the potential of pharmacological inhibition of amino acid transporters to inhibit T cell activation and the application of this strategy for treating immunological and inflammatory disorders. In the present review, we explore the possibility of targeting L-type amino acid transporter (LAT) as a novel therapeutic approach for bronchial asthma, including its steroid-resistant endotypes.
Clinical Role of Serum miR107 in Type 2 Diabetes and Related Risk Factors
Authors: Šimonienė Diana , Stukas Darius , Daukša Albertas , Veličkienė Džilda
Published: April 2022

Background: As the diagnostic and treatment options for diabetes improve, more attention nowadays is being paid to the exact identification of the etiopathological mechanism of type 2 diabetes (T2DM). Insulin resistance (IR) is a pathogenetic background for T2DM. Several studies demonstrate that miRNAs play an important role in systemic inflammation and thus in T2DM pathogenesis. Overexpression of miR-107 may cause an imbalance of glucose homeostasis, obesity, and dyslipidemia, by regulating insulin sensitivity through the insulin signaling pathway. Methods: 53 patients with T2DM and 54 nondiabetic patients were involved in the study. This study aimed to examine whether miR-107 expression in the serum of patients with diabetes was different from the control group (non-diabetic) and whether miR-107 expression correlated with lipid levels, BMI, and other factors, and finally, with insulin resistance in general. Results: miR-107 expression was higher in the T2DM group than in the control group (1.33 versus 0.63 (p = 0.016). In general, miR-107 expression was directly and positively associated with BMI (r = 0.3, p = 0.01), age (r = 0.3, p = 0.004), and male gender (p = 0.006). Moreover, miR-107 was related to dyslipidemia: Patients with higher miR-107 levels had lower HDL levels (in the control group: r = −0.262, p = 0.022 vs. diabetic group: r = −0.315, p = 0.007). Finally, the overexpression of miR-107 was associated with higher HOMA-IR in the diabetic group (r = 0.373, p = 0.035). Conclusion: MiR-107 expression is higher among diabetic patients than that of nondiabetic control subjects. Higher miR-107 levels are also related to dyslipidemia (lower HDL levels)—in the general cohort and non-diabetic subjects. Moreover, higher miR-107 expression is related to insulin resistance in the diabetic group. In general, higher miR-107 expression levels are related to a higher BMI, older age, and the male gender.
The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers
Authors: Currie Jean-Christophe , Demeule Michel , Charfi Cyndia , Zgheib Alain , Larocque Alain , Danalache Bogdan Alexandru , Ouanouki Amira , Béliveau Richard , Marsolais Christian , Annabi Borhane
Published: April 2022

Sortilin (SORT1) receptor-mediated endocytosis functions were exploited for this new approach for effective and safe treatments of gynecological cancers. Here, high expression of SORT1 was found in >75% of the clinically annotated ovarian and endometrial tumors analyzed by immunohistochemistry. Therefore, the anticancer properties of the peptide-drug conjugate TH1902, a peptide that targets SORT1 and which is linked to docetaxel molecules, were investigated both in vitro using ovarian and endometrial cancer cell cultures and in vivo using xenograft models. In vitro, TH1902 inhibited cell proliferation and triggered higher SORT1-dependent cell apoptosis than unconjugated docetaxel did in ES-2 and SKOV3 ovarian cancer cell lines. The uptake of the Alexa488-TH19P01 peptide from TH1902 was reduced upon siRNA-mediated silencing of SORT1. In vivo, weekly administration of TH1902 showed better tolerability compared to equivalent docetaxel doses and inhibited tumor growth in ovarian and endometrial xenograft mice models. TH1902 as a single agent inhibited ovarian tumor growth more than either of the unconjugated taxanes or carboplatin. Furthermore, TH1902 combination with carboplatin also demonstrated better efficacy when compared to both taxanes-carboplatin combinations. Overall, TH1902 shows better in vivo efficacy, compared to that of docetaxel and even paclitaxel, against SORT1-positive ovarian and endometrial cancers and could be safely combined with carboplatin.
Lymph but Not Blood Vessel Invasion Is Independent Prognostic in Lung Cancer Patients Treated by VATS-Lobectomy and Might Represent a Future Upstaging Factor for Early Stages
Authors: Biesinger Melanie , Eicken Nele , Varga Alexander , Weber Michael , Brndiar Milos , Erd Georg , Errhalt Peter , Hackner Klaus , Hintermair Sarah , Petter-Puchner Alexander , Scheed Axel , Stubenberger Elisabeth , Ghanim Bahil
Published: April 2022

Lung cancer is the most frequent cause of cancer-related death worldwide. The patient’s outcome depends on tumor size, lymph node involvement and metastatic spread at the time of diagnosis. The prognostic value of lymph and blood vessel invasion, however, is still insufficiently investigated. We retrospectively examined the invasion of lymph vessels and blood vessels separately as two possible prognostic factors in 160 patients who underwent a video-assisted thoracoscopic lobectomy for non-small-cell lung cancer at our institution between 2014 and 2019. Lymph vessel invasion was significantly associated with the UICC stage, lymph node involvement, tumor dedifferentiation, blood vessel invasion and recurrence. Blood vessel invasion tended to be negative prognostic, but missed the level of significance (p = 0.108). Lymph vessel invasion, on the other hand, proved to be a prognostic factor for both histological subtypes, adenocarcinoma (p < 0.001) as well as squamous cell carcinoma (p = 0.018). After multivariate analysis apart from the UICC stage, only lymph vessel invasion remained independently prognostic (p = 0.018). Remarkably, we found analogue survival curve progressions of patients with stage I, with lymph vessel invasion, compared to stage II non-small-cell lung cancer. After further validation in prospective studies, lymph vessel invasion might be considered as an upstaging factor in resectable lung cancer. Especially in the early-stage of the disease, it might represent an additional risk factor to consider adjuvant therapy after surgical resection.
Microstructure, Thermal and Mechanical Properties of Refractory Linings Modified with Polymer Fibers
Authors: Prochwicz Marcin , Czaja Paweł , Morgiel Jerzy , Czeppe Tomasz , Góral Anna
Published: April 2022

The reduction in the inherent brittleness of coatings applied on parts of ceramic shielding used for continuous steel casting (CSC) processes is highly desired, since it can significantly diminish losses occurring during post-application handling and mounting. One of such coatings, prepared mostly from fused silica, ludox, tabular alumina, chamotte, cenospheres, dextrine and aluminum powder, is known commercially as Thermacoat™. The present experiment is focused on the effect of the modification of its composition by rising the content of the cenospheres (max. 2.5 wt.%) or by introducing up to 1.5 wt.% of polymer Belmix™ fibers (~34 μm diameter/12 mm length) on the microstructure and mechanical properties. The maximum amount of introduced additions was limited by the accompanying loss of mass viscosity, which must allow for deposition through immersion. Next, the differential scanning calorimetry and differential thermogravimetric analysis techniques were employed to evaluate the extent of the weight change and heat response of the mass during the drying and annealing stages. The dried materials’ microstructure was investigated with light and scanning electron microscopy, while the chemical composition was studied by energy dispersive spectroscopy. Finally, a three-point flexural bending method was used to determine changes in the material mechanical properties. The performed experiments proved that the small addition (~1 wt.%) of polymer fibers is sufficient for the significant improvement of the Thermacoat™ green mechanical strength at ambient temperature, presenting a reproducible ultimate flexural strength of ~0.2 MPa.
Developments in the Management of Metastatic HER2-Positive Breast Cancer: A Review
Authors: Lebert Julie , Lilly Evan J.
Published: April 2022

Approximately 20% of breast cancers overexpress human epidermal growth factor receptor 2 (HER2), providing an actionable target for many different therapies. In the metastatic setting, prognosis has improved greatly with the use of anti-HER2 drugs such as trastuzumab, pertuzumab, and trastuzumab-emtansine. In the third line setting and beyond, several emerging treatments have shown benefits, including novel small molecule targeted agents and antibody-drug conjugates. Systemic treatment of brain metastases in HER2-positive patients and the role of endocrine-based treatment for patients with hormone receptor (HR) positive disease remain areas of research interest. This article will review the current approach to systemic management of metastatic HER2-positive breast cancer in Canada, and present novel treatments that may be available in the near future.
Energy Storage System Design in the Light of Multisource Solution from a Viability Analysis
Authors: Tavares Nascimento Viviane , Martinez-Bolaños Julio Romel , Morales Udaeta Miguel Edgar , Veiga Gimenes André L. , Riboldi Victor Baiochi , Ji Tuo
Published: April 2022

The objective of this work is to analyze the economic viability of a replacement to the energy supplied by the grid during costly periods by infrastructure owned by the consumer. The study takes into account the costs to purchase, manage and operate the structure to set which option an affordable option. A statistical approach based on the Monte Carlo Method distributes the costs through a 25 lifecycle period, establishing the cash flow and the economic parameters for each energy source combination scenario. The scenarios are evaluated for a Brazilian consumer, and the current regulatory parameters and market values are deployed to study the viability for a Diesel Generator, and two different types of batteries as replacements during the most energy-costly periods. As a result, the work establishes the most affordable scenarios from a technical and economic approach. Batteries are still costly for the consumer, and a 48.3% reduction in the costs may enable the deployment of batteries as an alternative in the country. The results establish that multi-source energy set with a Diesel Generator is an economically viable possibility during peak hours.
Housing, Transportation and Quality of Life among People with Mobility Limitations: A Critical Review of Relationships and Issues Related to Access to Home- and Community-Based Services
Authors: Best Krista L. , Noreau Luc , Gagnon Marie-Andrée , Barthod Corentin , Hitzig Sander L. , Routhier François
Published: April 2022

Anecdotal reports suggest that adequate housing and transportation could be fundamental elements required to ensure quality of life (QOL) for people with mobility limitations. Certain home- and community-based services (HCBS) are also necessary to ensure that housing and transportation needs are met. Understanding QOL as it relates to housing and transportation is critical for people with mobility limitations but requires appropriate assessment of these constructs. The aims of this research were to explore the relationships between housing and transportation on QOL for people with mobility limitations, to describe the current conceptual measurement issues and to propose dimensions of access that could facilitate assessment of QOL as it relates to housing, transportation and HCBS. A critical review of the literature was conducted by experts in disability, QOL and access theory. While evidence indicated a potential influence of housing and transportation on QOL for people with mobility limitations, the relationships between these concepts were weak and inconclusive. Moreover, the measurement tools used lacked appropriateness to specifically measure these constructs. Approaching these measurement issues within an access theory may better position future research to address the housing, transportation and HSBS needs of people with mobility limitations. Future research may consider elements of availability, accessibility, accommodation, affordability, acceptability and awareness to ensure access for people with mobility limitations. A better understanding of QOL as it relates to housing, transportation and HCBS will improve the quality of research, which may in turn improve access of adequate services for people with mobility limitations.
System for Game-like Therapy in Balance Issues Using Audiovisual Feedback and Force Platform
Authors: Janatová Markéta , Pětioký Jakub , Hoidekrová Kristýna , Veselý Tomáš , Hána Karel , Smrčka Pavel , Štěpánek Lubomír , Lippert-Grünerová Marcela , Jeřábek Jaroslav
Published: April 2022

Background: The aim of the work is to verify the usability of a stabilometric platform and audiovisual feedback in the group-based therapy of patients with vertebral algic syndrome, to analyze an immediate effect after a single therapeutic unit, and to analyze differences between male and female probands. Methods: The study included 189 patients (90 male, age 55 ± 12 and 89 female, age 52 ± 12). All patients received group balance therapy with a portable medical device, Homebalance MA. The intervention consisted of measurement of quiet stance and a reference training scene before and after 15 min of game-like balance training with audiovisual feedback. Results: A statistically significantly lower value of the body sway trajectory during quiet stance was detected in men than in women. After a single therapy session, there was a statistically significant improvement in quiet stance with visual feedback, and marginal statistically significant improvement in the time required to complete the reference training scene. Conclusions: Homebalance MA is a utilizable tool for group therapy. The use of group game-like balance training increases the availability of physiotherapeutic intervention for a larger number of patients, while maintaining the positive effect of the therapy.
A Saliency Prediction Model Based on Re-Parameterization and Channel Attention Mechanism
Authors: Yan Fei , Wang Zhiliang , Qi Siyu , Xiao Ruoxiu
Published: April 2022

Deep saliency models can effectively imitate the attention mechanism of human vision, and they perform considerably better than classical models that rely on handcrafted features. However, deep models also require higher-level information, such as context or emotional content, to further approach human performance. Therefore, this study proposes a multilevel saliency prediction network that aims to use a combination of spatial and channel information to find possible high-level features, further improving the performance of a saliency model. Firstly, we use a VGG style network with an identity block as the primary network architecture. With the help of re-parameterization, we can obtain rich features similar to multiscale networks and effectively reduce computational cost. Secondly, a subnetwork with a channel attention mechanism is designed to find potential saliency regions and possible high-level semantic information in an image. Finally, image spatial features and a channel enhancement vector are combined after quantization to improve the overall performance of the model. Compared with classical models and other deep models, our model exhibits superior overall performance.
An Auto Adjustable Transimpedance Readout System for Wearable Healthcare Devices
Authors: Park Hyusim , Lakshminarayana Shanthala , Pan Chenyun , Chung Hoon-Ju , Jung Sungyong
Published: April 2022

The objective of this work was to design a versatile readout circuit for patch-type wearable devices consisting of a Transimpedance Amplifier (TIA). The TIA performs Current to Voltage (I–V) conversion, the most widely used technique for amperometry and impedance measurement for various types of electrochemical sensors. The proposed readout circuit employs a digitally controllable feedback resistor (Rf) technique in the TIA to improve accuracy, which can be utilized in a variety of electrochemical sensors within a current range of 0.1 µA–100 µA. It is designed to accommodate multiple sensors simultaneously to track multiple target analytes for high accuracy and versatile usage. The readout circuit consists of low power operational amplifier (op–amp) and digital circuit blocks, is designed and fabricated with Magna 0.18 µm Complementary Metal Oxide Semiconductor (CMOS) technology, which provides low power consumption and a high degree of integration. The design has a small size of 0.282 mm2 and low power consumption of 0.38 mW with a 3.3 V power supply, which are desirable factors in wearable device applications.
Voltage Multiplier with High Input/Output Voltage Gain from Center-Tap Rectifier-Voltage Tripler and Quadrupler
Authors: Ryu Sang-Gyun , Park Chan-Bae , Lee Hyung-Woo , Lee Jae-Bum
Published: April 2022

This paper proposes new rectifiers in the center-tap transformer to provide higher input/output (I/O) voltage gain with an equal transformer turns-ratio in the LLC resonant converter: a voltage tripler rectifier and a voltage quadrupler rectifier, which have simple structures with one capacitor and two diodes, and three and four times higher I/O voltage gain than a center-tap rectifier, respectively. Each rectifier is compared in terms of the transformer turns-ratio and the magnetizing offset current, and the voltage and current stresses in the capacitors and diodes. The validity of these proposed rectifiers in the half-bridge (HB) LLC resonant converter is confirmed by the experimental results from a 100 V/200 W output prototype.
Maximizing Channel Capacity of 3D MIMO System via Antenna Downtilt Angle Adaptation Using a Q-Learning Algorithm
Authors: Lee Shu-Hung , Shi Xiao-Pei , Tan Tan-Hsu , Tung Yu-Che , Huang Yung-Fa
Published: April 2022

3D MIMO introduces the vertical dimension of the antenna downtilt angle to make the direction of signal transmission more accurate to improve system capacity. In this paper, we verify the effect of antenna downtilt angle on channel capacity through simulations of four fixed antenna downtilt angles, 90, 96, 99, and 102 degrees under the conditions that the distance between mobile station (MS) and base station (BS) is 250 m, and the heights of antenna in BS and MS are 25 m and 1.5 m, respectively. The simulation results show that the antenna downtilt angle of 96 degrees has a larger channel capacity than the others. In addition, we proposed an adaptive optimization method by applying the Q-learning algorithm to adaptively optimize the antenna downtilt angles to maximize system capacity. The performance of the proposed method is to investigate the Q-learning algorithm with three different discount rates at 0.9, 0.5, and 0.1, and four different propagation distances on 20 × 1 and 60 × 4 MIMO. We demonstrate that there is only a 1% difference between the adaptively optimized antenna downtilt angle and the ideal optimal antenna downtilt angle when the discount rate of Q-learning algorithm is 0.9, and its channel capacity performance can reach more than 99.72% of the ideal optimal one.
Balancing Public & Economic Health in Japan during the COVID-19 Pandemic: A Descriptive Analysis
Authors: Kim Gainha , Natuplag Justine M. , Jin Lin Sui , Feng Jinyi , Ray Nicolas
Published: April 2022

Despite loose restrictions and a low mortality rate due to COVID-19, Japan faced the challenge of stabilizing its economy during the pandemic. Here, we analyzed how the Japanese government attempted to maintain a balance between the health of the population and the health of the economy. We used a mix of quantitative data, information from policy documents, and news agency publications. Features of the Japanese government’s handling of the pandemic include the lack of constitutional authority to enforce a lockdown, the laxer restrictions compared with other countries in which citizens were advised only to exercise self-restraint and avoid close social contact, and the existence of expert panels that had only an advisory role. Our findings address the slow initial response of the government, which feared that the 2020 Tokyo Olympics would be canceled, and the increased testing when the Olympics were postponed, as well as the expansion of vaccination efforts after the Olympics. In addition, there was a targeted campaign to promote national travel to increase economic revenue in the tourism sector, but this led to an increase in COVID-19 cases.
Design and Evaluation of a Robust Observer Using Dead-Zone Lyapunov Functions—Application to Reaction Rate Estimation in Bioprocesses
Authors: Rincón Alejandro , Hoyos Fredy E. , Restrepo Gloria M.
Published: April 2022

This paper addresses the design and evaluation of a robust observer for second order bioprocesses considering unknown bounded disturbance terms and uncertainty in the dynamics of the unknown and known states. The observer design and the stability analysis are based on dead-zone Lyapunov functions, and a detailed procedure is provided. The transient response bounds and the convergence region of the unknown observer error are determined in terms of the disturbance bounds, considering persistent but bounded disturbances in the dynamics of both the known and unknown observer errors. This is a significant contribution to closely related observer design studies, in which the transient response bounds are determined, but persistent and bounded disturbances are not considered in the dynamics of the known observer error. Other important contributions are: (i) the procedure for defining the observer parameters is significantly simpler than common observer designs, since a solution to the Ricatti equation, solution to LMI constraints, or the accomplishment of eigenvalue inequality conditions are not required; (ii) discontinuous signals are not used in the observer; and (iii) the effect of the gain sign associated with the unknown state in the dynamics of the known state is explicitly and clearly considered in the observer design and in the convergence study. In addition, the guidelines for selecting the observer parameters are provided. Numerical simulation confirms the stability analysis results: the observer errors converge within a short time, with a low estimation error, if observer-parameters are properly defined.
Influence of Ripening Stage on Quality Parameters of Five Traditional Tomato Varieties Grown under Organic Conditions
Authors: Ayuso-Yuste María Concepción , González-Cebrino Francisco , Lozano-Ruiz Mercedes , Fernández-León Ana María , Bernalte-García María Josefa
Published: April 2022

Consumers demand traditional, tasty tomatoes in contrast to new hybrid cultivars that have poor sensory characteristics. Some physicochemical parameters (total soluble solids, pH, titratable acidity, color and firmness), functional properties (vitamin C, lycopene, β-carotene and total antioxidant activity) and sensory characteristics of five traditional tomato varieties (T1-T5) in three ripening stages, grown under organic conditions, were evaluated. These were compared to the commercial hybrid ‘Baghera’, grown in the same conditions. Firmness of all varieties declined and the color parameters L* and H* decreased, whereas a* and a*/b* increased progressively with ripening. Lycopene also increased with ripening in all varieties, with the highest content being 132.64 mg kg−1 fw for T4. All traditional tomato varieties were richer in lycopene than commercial ones in the two last ripening stages. Vitamin C content ranged widely between 27.33 and 267.27 mg kg−1 fw. Among the traditional varieties, T2 (BGV003524) stood out due to its highest total soluble solids and vitamin C contents, total antioxidant activity, and H* and a*/b*; this variety was also the most appreciated by panelists. These traditional varieties could be an alternative to commercial cultivars, as they have good quality characteristics and can satisfy consumer demand for organic produce.
The Gap in Community Sports: Utilization of Sports Facilities in South Korea
Authors: Kang Minuk , Lee Youngjik
Published: April 2022

Anyone can participate in sports, but not everyone has the opportunity to do so. The purpose of this study was to identify the factors causing inequality in sports participation based on the causes of the disparity in sports facility utilization in South Korea. Based on ecosystem theory, factors affecting the use of sports facilities were identified. For the causal relationship analysis of each factor, a hierarchical regression analysis was performed using the SPSS (version 26.0) package (IBM, Armonk, NY, USA). The characteristics of individual, family, and community levels show the different impacts based on study models with hierarchical structures. The results of this study illustrated that family characteristics did not influence the facilities’ utilization rate. However, individual and community characteristics did influence the sports facilities’ utilization rate. Although these results were derived from the case of South Korea, they are comparable data focusing on country-specific characteristics and community indicators. It is expected that sports participation can be strengthened by bridging the gap in sports facilities’ utilization.
The Immunomodulatory Effects of Statins on Macrophages
Authors: Sheridan Alanah , Wheeler-Jones Caroline P. D. , Gage Matthew C.
Published: April 2022

Statins are 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors used worldwide to manage dyslipidaemia and thus limit the development of atherosclerotic disease and its complications. These atheroprotective drugs are now known to exert pleiotropic actions outside of their cholesterol-lowering activity, including altering immune cell function. Macrophages are phagocytic leukocytes that play critical functional roles in the pathogenesis of atherosclerosis and are directly targeted by statins. Early studies documented the anti-inflammatory effects of statins on macrophages, but emerging evidence suggests that these drugs can also enhance pro-inflammatory macrophage responses, creating an unresolved paradox. This review comprehensively examines the in vitro, in vivo, and clinical literature to document the statin-induced changes in macrophage polarization and immunomodulatory functions, explore the underlying mechanisms involved, and offer potential explanations for this paradox. A better understanding of the immunomodulatory actions of statins on macrophages should pave the way for the development of novel therapeutic approaches to manage atherosclerosis and other chronic diseases and conditions characterised by unresolved inflammation.
Real-Time Spiral CMR Is Superior to Conventional Segmented Cine-Imaging for Left-Ventricular Functional Assessment in Patients with Arrhythmia
Authors: Nita Nicoleta , Kersten Johannes , Pott Alexander , Weber Fabian , Tesfay Temsgen , Benea Marius-Tudor , Metze Patrick , Li Hao , Rottbauer Wolfgang , Rasche Volker , Buckert Dominik
Published: April 2022

(1) Background: Segmented Cartesian Cardiovascular magnetic resonance (CMR) often fails to deliver robust assessment of cardiac function in patients with arrhythmia. We aimed to assess the performance of a tiny golden-angle spiral real-time CMR sequence at 1.5 T for left-ventricular (LV) volumetry in patients with irregular heart rhythm; (2) Methods: We validated the real-time sequence against the standard breath-hold segmented Cartesian sequence in 32 patients, of whom 11 presented with arrhythmia. End-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and ejection fraction (EF) were assessed. In arrhythmic patients, real-time and standard Cartesian acquisitions were compared against a reference echocardiographic modality; (3) Results: In patients with sinus rhythm, good agreements and correlations were found between the segmented and real-time methods, with only minor, non-significant underestimation of EDV for the real-time sequence (135.95 ± 30 mL vs. 137.15 ± 31, p = 0.164). In patients with arrhythmia, spiral real-time CMR yielded superior image quality to the conventional segmented imaging, allowing for excellent agreement with the reference echocardiographic volumetry. In contrast, in this cohort, standard Cartesian CMR showed significant underestimation of LV-ESV (106.72 ± 63.51 mL vs. 125.47 ± 72.41 mL, p = 0.026) and overestimation of LVEF (42.96 ± 10.81% vs. 39.02 ± 11.72%, p = 0.039); (4) Conclusions: Real-time spiral CMR improves image quality in arrhythmic patients, allowing reliable assessment of LV volumetry.
Literature Review of Cervical Regeneration after Loop Electrosurgical Excision Procedure, and Study Project (CeVaLEP) Proposal
Authors: Lūse Laura , Urtāne Anda Ķīvīte , Lisovaja Ija , Jermakova Irina , Donders Gilbert G. G. , Vedmedovska Natālija
Published: April 2022

Objective: To compile existing knowledge on the level of cervical regeneration (detected by ultrasound) after loop electrosurgical excision procedure (LEEP) and to suggest research protocol for further studies. Methods: We conducted a literature search of Medline, Web of Science, Scopus, and Cochrane databases using the keywords “cervix” and “regeneration” without year restrictions. Our eligibility criteria included studies that analysed cervical volume and length regeneration using ultrasound. A literature review was conducted following PRISMA guidelines and registered in PROSPERO (reg. no. CRD42021264062). Information about the studies was extracted from each analysed study on an Excel datasheet and the average regeneration with standard deviation was calculated. All included studies’ possible biases were assessed by the National Institutes of Health’s (NIH) quality assessment tool. Results: The literature search identified 802 papers and four trials (n = 309) that met our criteria. They investigated cervical length and volume regeneration after LEEP using ultrasound, concluding that there is a profound regeneration deficit. Average cervical length regeneration after 6 months was 83.4% (±10.8%) and volume regeneration was 87.4% (±6.1%). All analysed studies had their biases; therefore, based on the conducted studies’ protocols, we present a CeVaLEP research protocol to guide high-quality studies. Conclusion: After LEEP, there is a cervical regeneration deficit. There is a lack of high-quality studies that assess cervical volume regeneration and its relation to obstetrical outcomes. There is a gap in the field and more research is needed to define the prenatal risks related to cervical regeneration.
A Comparative Study of Nine SARS-CoV-2 IgG Lateral Flow Assays Using Both Post-Infection and Post-Vaccination Samples
Authors: Mulder Leontine , Carrères Benoit , Muggli Franco , Zollinger Alix , Corthésy John , Klijn Adrianne , Togni Giuseppe
Published: April 2022

Background: Since the SARS-CoV-2 pandemic, lateral flow assays (LFA) detecting specific antibodies have entered the market in abundance. Despite being CE-IVD-labeled, the antigenic compounds of the assays are often unknown, the performance characteristics provided by the manufacturer are often incomplete, and the samples used to obtain the data are not detailed. Objective: To perform a comparative evaluation of nine lateral flow assays to detect IgG responses against SARS-CoV-2. For the evaluation, a carefully designed serum panel containing post-infection samples and post-vaccination (both mRNA vaccine and inactivated virus vaccine) samples was used. Results: The sensitivity of the assays overall ranged from 9 to 90.3% and the specificity ranged from 94.2 to 100%. Spike protein-containing assays performed generally better than the assays with only nucleocapsid protein. The sensitivity of some assays was higher on post-infection samples, while other assays had a higher sensitivity to post-vaccination samples. Conclusion: A comparative approach in the verification of LFAs with an adequately designed serum panel enabled the identification of the antigens used in the assays. Sensitivities differed between post-infection and post-vaccination samples, depending on the assays used. This demonstrates that the verification of assays must be performed with samples representative of the intended use of the assay.
Poly(Lactic Acid) (PLA)-Based Nanocomposites: Impact of Vermiculite, Silver, and Graphene Oxide on Thermal Stability, Isothermal Crystallization, and Local Mechanical Behavior
Authors: Khammassi Sabrine , Tarfaoui Mostapha , Škrlová Kateřina , Měřínská Dagmar , Plachá Daniela , Erchiqui Fouad
Published: April 2022

The structural, thermal, and mechanical properties of unreinforced and reinforced polylactic acid (PLA) were investigated. The PLA was a biopolymer that was reinforced with four fillers (i.e., graphene oxide (GO) and silver (Ag); vermiculite (VMT) and silver (Ag); and two organically modified vermiculites). The processing technique for the production of the composite materials were carefully planned. The PLA nanocomposites were investigated by examining their morphological aspects, changes in PLA phases and transitions and, most importantly, the effect on certain final properties. X-ray diffraction and differential scanning calorimetry (DSC) analysis indicated that the sample was completely amorphous. Thermogravimetric analysis (TGA) results indicated that the presence of reinforcing particles in the PLA matrix did not affect the thermal degradation of these composites. Furthermore, the local mechanical properties were investigated using the microindentation method to evaluate the effect of different nanofillers. Scanning electron microscopy (SEM) and a VHX-500 optical digital microscope (Keyence International, Mechelen, Belgium) were also used to examine the surface morphology of the PLA polymer composites. These results can help to select suitable fillers to enhance the PLA performance of biopolymers.
Metatranscriptomic Analyses Reveal the Functional Role of Botrytis cinerea in Biochemical and Textural Changes during Noble Rot of Grapevines
Authors: Hegyi Ádám István , Otto Margot , Geml József , Hegyi-Kaló Júlia , Kun József , Gyenesei Attila , Pierneef Rian , Váczy Kálmán Zoltán
Published: April 2022

Botrytis cinerea, can lead to the formation of noble rot (NR) of grape berries under certain environmental conditions, resulting in favored metabolic and physical changes necessary for producing highly regarded botrytized wines. The functional genes involved in the textural and biochemical processes are still poorly characterized. We generated and analyzed metatranscriptomic data from healthy (H) berries and from berries representing the four stages of NR from the Tokaj wine region in Hungary over three months. A weighted gene co-expression network analysis (WGCNA) was conducted to link B. cinerea functional genes to grape berry physical parameters berry hardness (BH), berry skin break force (F_sk), berry skin elasticity (E_sk), and the skin break energy (W_sk). Clustered modules showed that genes involved in carbohydrate and protein metabolism were significantly enriched in NR, highlighting their importance in the grape berry structural integrity. Carbohydrate active enzymes were particularly up-regulated at the onset of NR (during the transition from phase I to II) suggesting that the major structural changes occur early in the NR process. In addition, we identified genes expressed throughout the NR process belonging to enriched pathways that allow B. cinerea to dominate and proliferate during this state, including sulphate metabolizing genes and genes involved in the synthesis of antimicrobials.
Role of Mineralocorticoid Receptor Antagonists in Diabetic Kidney Disease
Authors: Alexandrou Maria-Eleni , Theodorakopoulou Marieta P. , Sarafidis Pantelis A.
Published: April 2022

Diabetic kidney disease (DKD) represents a major public health issue, currently posing an important burden on healthcare systems. Renin–angiotensin system (RAS) blockers are considered the cornerstone of treatment of albuminuric DKD. However, a high residual risk of progression to more advanced CKD stages under RAS blockade still remains, while relevant studies did not show significant declines in cardiovascular events with these agents in patients with DKD. Among several other pharmacological classes, mineralocorticoid receptor antagonists (MRAs) have received increasing interest, due to a growing body of high-quality evidence showing that spironolactone and eplerenone can significantly lower blood pressure and albuminuria in patients with CKD. Furthermore, finerenone, a novel nonsteroidal MRA with unique physicochemical properties, was shown to effectively reduce cardiovascular events and death, as well as the incidence of end-stage kidney disease in patients with type 2 diabetes. This review discusses previous and recent clinical evidence on the issue of nephroprotection and cardioprotection in DKD offered by mineralocorticoid receptor antagonism, aiming to aid clinicians in their treatment decisions for diabetic patients.
Identification of Cell Markers and Their Expression Patterns in Skin Based on Single-Cell RNA-Sequencing Profiles
Authors: Zhou Xianchao , Ding Shijian , Wang Deling , Chen Lei , Feng Kaiyan , Huang Tao , Li Zhandong , Cai Yudong
Published: April 2022

Atopic dermatitis and psoriasis are members of a family of inflammatory skin disorders. Cellular immune responses in skin tissues contribute to the development of these diseases. However, their underlying immune mechanisms remain to be fully elucidated. We developed a computational pipeline for analyzing the single-cell RNA-sequencing profiles of the Human Cell Atlas skin dataset to investigate the pathological mechanisms of skin diseases. First, we applied the maximum relevance criterion and the Boruta feature selection method to exclude irrelevant gene features from the single-cell gene expression profiles of inflammatory skin disease samples and healthy controls. The retained gene features were ranked by using the Monte Carlo feature selection method on the basis of their importance, and a feature list was compiled. This list was then introduced into the incremental feature selection method that combined the decision tree and random forest algorithms to extract important cell markers and thus build excellent classifiers and decision rules. These cell markers and their expression patterns have been analyzed and validated in recent studies and are potential therapeutic and diagnostic targets for skin diseases because their expression affects the pathogenesis of inflammatory skin diseases.
Effect of Natural Deep Eutectic Solvents on trans-Resveratrol Photo-Chemical Induced Isomerization and 2,4,6-Trihydroxyphenanthrene Electro-Cyclic Formation
Authors: Mattioli Roberto , Di Risola Daniel , Federico Rodolfo , Ciogli Alessia , Gasparrini Francesco , Villani Claudio , Fontana Mario , Maggiore Anna , d’Erme Maria , Mosca Luciana , Francioso Antonio
Published: April 2022

trans-Resveratrol is a natural bioactive compound with well-recognized health promoting effects. When exposed to UV light, this compound can undergo a photochemically induced trans/cis isomerization and a 6π electrochemical cyclization with the subsequent formation of 2,4,6-trihydroxyphenanthrene (THP). THP is a potentially harmful compound which can exert genotoxic effects. In this work we improved the chromatographic separation and determination of the two resveratrol isomers and of THP by using a non-commercial pentafluorophenyl stationary phase. We assessed the effect of natural deep eutectic solvents (NaDES) as possible photo-protective agents by evaluating cis-resveratrol isomer and THP formation under different UV-light exposure conditions with the aim of enhancing resveratrol photostability and inhibiting THP production. Our results demonstrate a marked photoprotective effect exerted by glycerol-containing NaDES, and in particular by proline/glycerol NaDES, which exerts a strong inhibitory effect on the photochemical isomerization of resveratrol and significantly limits the formation of the toxic derivative THP. Considering the presence of resveratrol in various commercial products, these results are of note in view of the potential genotoxic risk associated with its photochemical degradation products and in view of the need for the development of green, eco-sustainable and biocompatible resveratrol photo-stable formulations.