bluuebunny/crossref_metadata_2025 huggingface dataset:
Unraveling Alzheimer’s disease: insights from single-cell sequencing and spatial transcriptomic
Authors: He Yibo , Lu Wenqiang , Zhou Xiao , Mu Jie , Shen Wei
Published: December 2024

Alzheimer’s disease (AD) is a neurodegenerative disorder marked by cognitive decline, primarily affecting memory and executive function. This review highlights recent advancements in single-cell sequencing and spatial transcriptomics, which provide detailed insights into the cellular heterogeneity and neuroimmune mechanisms of AD. The review addresses the need for understanding complex cellular interactions to identify novel therapeutic targets and biomarkers. Single-cell sequencing has revolutionized our understanding by mapping gene expression at the individual cell level, revealing distinct microglial and astrocytic states that contribute to neuroinflammation and neurodegeneration. These technologies have uncovered disease-associated microglial subpopulations and gene expression changes linked to AD risk genes, essential for developing targeted therapies. In conclusion, the integration of single-cell and spatial transcriptomics with other omics data is crucial for a comprehensive understanding of AD, paving the way for personalized medicine. Continued interdisciplinary collaboration will be vital in translating these findings into effective treatments, improving patient outcomes.
Association between body roundness index and female infertility: a cross-sectional study of NHANES 2013–2018
Authors: Li Haiyan , OuYang Zhenbo , Ding Ziyao , Hu Xianyue , Bao Yanjing , Gao Tianyang , Hua Wenfeng
Published: December 2024

Background

The use of visceral obesity as an indicator for predicting female infertility risk has not been well established. The body roundness index (BRI) is a novel, non-invasive indicator of visceral fat; however, previous reports have not addressed the relationship between the BRI and female infertility. This study sought to fill this research gap by investigating the association between the BRI and the risk of female infertility.

Methods

This cross-sectional study examined 3,528 women aged 18 to 45 who participated in the National Health and Nutrition Examination Survey (NHANES) from 2013 to 2018. Infertility was defined based on responses to the reproductive health questionnaire. The BRI was calculated using waist circumference and height. Covariates included demographic traits, physical exam results, laboratory test findings, and survey data. Weighted multivariable logistic regression models and spline smoothing analysis assessed the relationship between the BRI and infertility. Bayesian statistics were used to examine the robustness of significant associations.

Results

Based on their self-report data, 407 (11.54%) participants were classified as having infertility. A significantly higher percentage of participants with a higher BRI were found to have infertility. Multivariable logistic regression revealed that the BRI was significantly associated with increased female infertility risk, regardless of independent variable analysis by continuous variable or quartile (Q1 to Q4) in the fully adjusted model (Model 3, continuous variable: OR = 1.1, 95% confidence interval [CI] = 1.05–1.16, p = 0.0009; Q4 vs. Q1: OR = 2.16, 95% CI = 1.38–3.39, p = 0.0035, Ptrend = 0.004). Non-linear and threshold effects in the relationship between the BRI and female infertility were identified, with an inflection point of 6.36. Subgroup analyses showed that this positive association remained consistent across most demographic and health-related categories. The Bayesian statistics analyses further confirmed the robustness of these findings.

Conclusion

A positive non-linear relationship exists between the BRI and the risk of female infertility, suggesting that the BRI could serve as a valuable indicator in female fertility assessments.
Application of Mendelian randomized research method in oncology research: bibliometric analysis
Authors: Chen Jiahao , Wang Yunli , Jiang Rongsheng , Qu Yawei , Li Yan , Zhang Yang
Published: December 2024

Background

Cancer has always been a difficult problem in the medical field, and with the gradual deepening of Genome-wide association studies (GWAS), Mendelian randomization methods have been increasingly used to study cancer pathogenesis. In this study, we examine the literature on Mendelian cancer, summarize the status of the research, and analyze the development trends in the field.

Methods

Publications on “Mendelian Randomization - Cancer” were retrieved and downloaded from the Web of Science Core Collection database. CiteSpace 6.2.R4, VOSviewer 1.6.19, Scimago Graphica 1.0.38, Bibliometrix R-package, and a bibliometric online analysis platform were used for data analysis and visualization. An in-depth analysis of country or region, authors, journals, keywords, and references was performed to provide insights into the content related to the field.

Results

A total of 836 articles were included in the analysis; 643 authors from 72 countries had published articles related to the field. China and Harvard University (among countries and institutions, respectively) had the highest number of articles. Martin, Richard M and Smith, George Davey were the largest contributors. A total of 27 cancers have been studied, with breast, colorectal, and liver cancers being the most studied.

Conclusion

This study is the first to use bibliometric methods to visualize the application of Mendelian randomization analysis in the field of cancer, revealing research trends and research frontiers in the field. This information will provide a strong reference for cancer researchers and epidemiologic researchers.
Case report: Achieving significant tumor reduction in advanced pancreatic adenocarcinoma
Authors: Liu Hongying , Wang Yun , Zhang Qian , Ke Nengwen
Published: December 2024

Pancreatic cancer remains a highly malignant and challenging tumor with a dismal 5-year survival rate of only 13%. The majority of patients are diagnosed at advanced stages, where surgical options are limited, and prognosis is poor. Immunotherapy, particularly PD-1 inhibitors, has shown limited success in pancreatic cancer due to its unique tumor immune microenvironment. However, certain genetic profiles, such as BRCA1/2 mutations, high tumor mutational burden (TMB), or microsatellite instability-high (MSI-H), may enhance sensitivity to these therapies. This report presents two cases of advanced pancreatic cancer with BRCA1/2 mutations treated with a combination of chemotherapy and immune checkpoint inhibitors. The first patient, with TMB-H and stable microsatellites, achieved complete remission after conversion therapy and remains disease-free for over two years post-surgery. The second patient, with MSI-H and low TMB, experienced significant tumor regression and improved quality of life with a prolonged progression-free survival, although the patient ultimately declined surgery. These cases suggest that combined chemotherapy and immunotherapy may offer a promising treatment option for select pancreatic cancer patients, particularly those with specific genetic profiles, warranting further investigation into personalized approaches to immunotherapy in this malignancy.
Differentiation of histological calcification classifications in breast cancer using ultrashort echo time and chemical shift-encoded imaging MRI
Authors: Ayoub Yazan , Cheung Sai Man , Maglan Boddor , Senn Nicholas , Chan Kwok-Shing , He Jiabao
Published: December 2024

Introduction

Ductal carcinoma in situ (DCIS) accounts for 25% of newly diagnosed breast cancer cases with only 14%–53% developing into invasive ductal carcinoma (IDC), but currently overtreated due to inadequate accuracy of mammography. Subtypes of calcification, discernible from histology, has been suggested to have prognostic value in DCIS, while the lipid composition of saturated and unsaturated fatty acids may be altered in de novo synthesis with potential sensitivity to the difference between DCIS and IDC. We therefore set out to examine calcification using ultra short echo time (UTE) MRI and lipid composition using chemical shift-encoded imaging (CSEI), as markers for histological calcification classification, in the initial ex vivo step towards in vivo application.

Methods

Twenty female patients, with mean age (range) of 57 (35–78) years, participated in the study. Intra- and peri-tumoural degree of calcification and peri-tumoural lipid composition were acquired on MRI using UTE and CSEI, respectively. Ex vivo imaging was conducted on the freshly excised breast tumour specimens immediately after surgery. Histopathological analysis was conducted to determine the calcification status, Nottingham Prognostic Index (NPI), and proliferative activity marker Ki-67.

Results

Intra-tumoural degree of calcification in malignant classification (1.05 ± 0.13) was significantly higher (p = 0.012) against no calcification classification (0.84 ± 0.09). Peri-tumoural degree of calcification in malignant classification (1.64 ± 0.10) was significantly higher (p = 0.033) against no calcification classification (1.41 ± 0.18). Peri-tumoural MUFA in malignant classification (0.40 ± 0.01) was significantly higher (p = 0.039) against no calcification classification (0.38 ± 0.02). Ki-67 showed significant negative correlation against peri-tumoural MUFA (p = 0.043, ρ = −0.457), significant positive correlation against SFA (p = 0.008, ρ = 0.577), and significant negative correlation against PUFA (p = 0.002, ρ = −0.653).

Conclusion

The intra- and peri-tumoural degree of calcification and peri-tumoural MUFA are sensitive to histological calcification classes supporting future investigation into DCIS prognosis.
A portrait of germline pathogenic variants in high and moderate penetrance breast cancer genes in Brazil
Authors: Oliveira Leandro Jonata de Carvalho , Rodrigues Amanda Muniz , Fernandes Carolina de Bustamante , Ramos do Rego Fernanda Orpinelli , Koyama Fernanda Christtanini , Souto Andreza Karine de Barros Almeida , Santana Thaiana Aragão , Gonzaga de Faria João Paulo , Lima Bulcão Marcela , Nascimento Ivana Lucia de Oliveira , Silva Ana Carolina Branco Neves , Gonçalves Isabela Pessoa Elias , Maia Rayana Elias , Azevedo Renata Gondim Meira Velame de , Galindo Layla Testa , Pachito Daniela Vianna , Cury Adriana , Zalis Mariano Gustavo , Ferrari Bruno Lemos , Garicochea Bernardo , Dienstmann Rodrigo
Published: December 2024

Introduction

The prevalence of germline pathogenic/likely pathogenic variants (P/LP) in high and moderate penetrance (HMP) genes is approximately 7%–10% among breast cancer (BC) patients. The prevalence and spectrum of BC P/LP variants are affected by several factors. There are limited genetic data from Brazilian patients with BC.

Methods

This is a retrospective cross-sectional study that aims to evaluate the germline profile of P/LP variants in 13 HMP BC genes (BRCA1, BRCA2, PALB2, TP53, CDH1, NF1, PTEN, STK11, CHEK2, ATM, BARD1, RAD51C, and RAD51D) in patients diagnosed with BC in Brazil. All patients were tested using multigene NGS panels covering from 35 to 105 genes. Primary endpoint was the prevalence of P/LP variants in BRCA1/2 and in other HMP genes. Secondary endpoints were stratified analyses according to age and BC subtype.

Results

This cohort involved 2,208 patients with BC from 2019 to 2023. Most patients (79.7%) were from Southeastern Brazil. The median age at genetic testing was 47 years, and most patients (59.4%) were ≤50 years. The BC subtype was available in 641 cases: 264 patients (41.2%) were HR+/HER2−, 116 (18.1%) were HER2+, and 261 (40.7%) had triple-negative breast cancer (TNBC). Overall, 215 (9.7%) had a P/LP in HMP genes, including 5.8% in BRCA1/2. The most frequent variants were found in BRCA2, BRCA1, and TP53. The founder variant R337H accounted for 79% of all TP53 pathogenic variants, representing 1% of the overall population. Deleterious variants in BRCA1/2 were more common in patients ≤50 years (7.7%) and TNBC (10.7%). In other HMP BC genes, the prevalence of P/LP variants did not significantly vary according to age and BC molecular subtype. The overall VUS rate in HMP genes was 19.6%.

Conclusion

In Brazil, the epidemiology of deleterious variants in HMP is comparable to published US and EU cohorts. The Brazilian TP53 R337H is a prevalent variant in BC patients. Deleterious BRCA1/2 variants vary according to age and BC subtype. Our study gives a broader understanding of BC risk genes and has opened doors to optimized testing and surveillance strategies in Brazil.
Mitigating inflammation and fibrosis: the therapeutic potential of quercetin liposomes in COPD
Authors: Yin Changfeng , Tian Yushan , Yan An , Wang Hongjuan , Lu Fengjun , Li Xianmei , Li Xiao , Han Shulei , Miao Ruijuan , Chen Huan , Li Di , Hou Hongwei , Hu Qingyuan
Published: December 2024

Introduction

Chronic obstructive pulmonary disease (COPD) is a disease with severe therapeutic obstacles and high worldwide death rate. COPD progresses predominantly through inflammatory response followed by fibrotic destruction. Quercetin (Que), recognized for its anti-inflammatory effects, presents significant promise as a therapeutic candidate for COPD therapy. However, poor water solubility and low bioavailability of Que hinder its further clinical application. Liposomes are renowned for their unique structure and function, which provided an efficient approach for the delivery of Que in various drug delivery systems. This study was aim to prepare a novel Que liposome (Que-lipo) and administrated via intratracheal (i.t.) with cigarette smoke induced COPD mice. The underlying therapeutic mechanisms against lung damage of Que-lipo were explored.

Methods

Que-lipo were prepared based on thin film dispersion method and administrated via intratracheal administration. The cigarette smoke induced COPD mice were established and a comprehensive approach was employed to explore the inflammation, pulmonary function and histopathology of lung after i.t. administration of Que-lipo, including enzyme-linked immunosorbent assay, histopathology and immunohistochemistry, reverse transcription-quantitative polymerase chain reaction.

Results and discussion

Que-lipo not only improved the solubility and biocompatibility of Que but also demonstrated effective cellular uptake in vitro. The inflammation, pulmonary function and pathological condition of lung were improved after i.t. administration of Que-lipo. Que-lipo also regulated the expression of key apoptosis-associated proteins such as Bcl-2 and caspase-3/7, leading to significant inhibition of apoptotic activity in COPD. Furthermore, Que-lipo markedly enhanced its ability to alleviate lung inflammation and fibrosis symptoms by modulating inflammation-related factors and fibrosis signaling molecules. The potential mechanisms of Que-lipo in treating COPD were elucidated, including the suppression of the NLRP3/IL-1β inflammasome pathway and the TGF-β1-related fibrosis signaling pathway.
Effects of individualized resistance training prescription with heart rate variability on muscle strength, muscle size and functional performance in older women
Authors: Bittencourt Diego , de Oliveira Ramon Martins , da Silva Deivid Gomes , Bergamasco João Guilherme Almeida , Cesar Marcelo de Castro , Godoi Jacomassi Daniela , de Camargo Júlio Benvenutti Bueno , Kingsley J. Derek , Libardi Cleiton Augusto
Published: December 2024

Introduction

This study aimed to investigate whether individualizing autonomic recovery periods between resistance training (RT) sessions (IND) using heart rate variability (HRV), measured by the root mean square of successive R-R interval differences (RMSSD), would lead to greater and more consistent improvements in muscle strength, muscle mass, and functional performance in older women compared to a fixed recovery protocol (FIX).

Methods

Twenty-one older women (age 66.0 ± 5.0 years old) were randomized into two different protocols (IND: n = 11; FIX: n = 10) and completed 7 weeks of RT. Measurements of RMSSD were performed within a five-day period to establish baseline values. The RMSSD values determined whether participants were recovered from the previous session. The assessments included muscle cross-sectional area (CSA), one-repetition maximum (1RM), peak torque (PT), rate of force development (RFD), chair stand (CS), timed up and go (TUG), 6-minutes walking (6MW), and maximum gait speed (MGS).

Results

There were no significant (P > 0.05) group vs. time interactions. There were significant main effects of time (P < 0.05) for CSA, 1RM, PT, TUG, CS, 6MW, and MGS, while no significant changes were observed for RFD (P > 0.05).

Conclusion

IND does not seem to enhance responses in muscle mass, strength, and functional performance compared FIX in healthy older women.
Engineering Nicotiana benthamiana for chrysoeriol production using synthetic biology approaches
Authors: Lee Saet Buyl , Lee Sung-eun , Lee Hyo , Kim Ji-Su , Choi Hyoseon , Lee Sichul , Kim Beom-Gi
Published: December 2024

Flavonoids are prevalent plant secondary metabolites with a broad range of biological activities. Their antioxidant, anti-inflammatory, and anti-cancer activities make flavonoids widely useful in a variety of industries, including the pharmaceutical and health food industries. However, many flavonoids occur at only low concentrations in plants, and they are difficult to synthesize chemically due to their structural complexity. To address these difficulties, new technologies have been employed to enhance the production of flavonoids in vivo. In this study, we used synthetic biology techniques to produce the methylated flavone chrysoeriol in Nicotiana benthamiana leaves. The chrysoeriol biosynthetic pathway consists of eight catalytic steps. However, using an Agrobacterium-mediated transient expression assay to examine the in planta activities of genes of interest, we shortened this pathway to four steps catalyzed by five enzymes. Co-expression of these five enzymes in N. benthamiana leaves resulted in de novo chrysoeriol production. Chrysoeriol production was unaffected by the Agrobacterium cell density used for agroinfiltration and increased over time, peaking at 10 days after infiltration. Chrysoeriol accumulation in agroinfiltrated N. benthamiana leaves was associated with increased antioxidant activity, a typical property of flavones. Taken together, our results demonstrate that synthetic biology represents a practical method for engineering plants to produce substantial amounts of flavonoids and flavonoid derivatives without the need for exogenous substrates.
Eastern filbert blight resistant Corylus avellana identified from 20 years of germplasm introduction and evaluation at Rutgers University, New Jersey, USA
Authors: Jacobs Daniel C. , Revord Ronald S. , Capik John M. , Molnar Thomas J.
Published: December 2024

The stem canker disease eastern filbert blight (EFB), caused by Anisogramma anomala, is a major impediment of European hazelnut (Corylus avellana) production in the United States. While most European hazelnut cultivars are highly susceptible to the pathogen, which remains confined to North America, EFB resistant and tolerant genotypes occur in the gene pool at low frequency. At Rutgers University, New Brunswick, NJ, USA, 5,226 trees were grown from open pollinated seeds collected from Russia, Crimea, Poland, Turkey, Estonia, Latvia, Lithuania, Moldova, Azerbaijan, Italy, and the Republic of Georgia between 2002 to 2010. The trees were field planted, exposed to A. anomala under high pathogen pressure, and evaluated for disease response 5-6 years after their establishment. At this point, around four percent were found to be EFB resistant totaling 216 accessions that spanned a wide diversity of seedlots from most countries and regions. However, recent observations show many of these once-resistant selections have since succumbed to EFB. In this study, the long-term disease response of this germplasm was evaluated to identify trees remaining resistant and tolerant and document changes in EFB response over time in relation to their origin. All trees were rated for presence of EFB according to a scale of 0 to 5 where 0 = no EFB and 5 = all stems have cankers. Data were assembled from three sets: first reports from 5-6 years after each planting year, a reassessment in 2017, and a final evaluation in January 2024. Overall, the results showed a significant reduction in resistant individuals from the original reports. By 2017, the population of 216 trees was reduced to 154 and by 2024 it decreased further to 91. Notably, this shift from resistant to susceptible phenotype was severe and abrupt and patterns were observed within related seed lots. These patterns were also apparent in trees where their resistance (R) genes were mapped. Specifically, all selections with R genes mapped to linkage group (LG) 6 now expressed severe EFB, while those with R genes mapped to LG 2 or 7 remained free of disease. These results strongly suggest pathogenic variation present over time played a role in the breakdown of resistance. Fortunately, despite loss of some of the germplasm, a wide variety of trees spanning most collection origins still remain free of EFB. These 91 trees from 56 distinct seedlots originating from 7 countries are formally documented in this manuscript to facilitate their long-term preservation, continued evaluation and sharing, and to increase global awareness of this valuable genetic resource for future research and breeding.
Metaphorical discourse in Beijing Winter Olympic news: a Trinocular Perspective analysis of language, cognition, and social functions
Authors: Peng Wei , Li Qingping
Published: December 2024

This study utilizes the “Trinocular Perspective” metaphor analysis framework to delve into the language, cognition, and social functions of metaphors in news coverage of the Beijing Winter Olympics and their interactions, thereby revealing the role of metaphors in shaping and articulating the narratives within Olympic news communication. The research indicated that, at the linguistic level, metaphors enhanced the expressiveness and appeal of news discourse through diverse forms and dense distribution. At the cognitive level, metaphors fostered a deeper public understanding of abstract concepts such as the Olympic spirit, the values of a community with a shared future for mankind, China’s national image, and the concept of sustainable development. On the social function level, metaphors will play a pivotal role in building social identity, disseminating values, highlighting social issues, and promoting social change. The language, cognition, and social functions of metaphors were closely interwoven, with linguistic expressions enriching cognitive frameworks, cognitive frameworks guiding the internalization and dissemination of social values, and social functions guiding public cognition through the strategic use of metaphorical language. This study reveals the critical function of metaphors in elevating the narrative richness and dissemination efficacy within the journalism of large-scale international sports competitions.
The greatest challenge in contemplative science: tailoring meditative practices
Authors: Garcia-Campayo Javier , Whyte Roberto , Hijar-Aguinaga Rinchen
Published: December 2024

The practice of meditation has grown in popularity around the world in recent decades. However, standard and untailored meditative practices are recommended for all practitioners, regardless of their personality traits or characteristics. No scientific attempts have been made to match specific meditation types with personality. This paper summarizes the limited proposals relevant to this subject in contemplative traditions such as Buddhism and schools of humanistic and transpersonal thought, including the Enneagram. We also discuss future research directions in this field.
“We are not ready for this”: physicians’ perceptions on climate change information and adaptation strategies - qualitative study in Portugal
Authors: Ponte Nidia , Alves Fátima , Vidal Diogo Guedes
Published: December 2024

Background

Climate change presents several challenges to public health and its professionals. This article aims to fill a significant gap in the current literature by understanding physicians’ perceptions of their role in educating others about health adaptation to climate change. It also explores their knowledge of health policies related to this issue in Portugal and their perceived influence on the development of adaptation policies at both local and national levels within the health sector.

Methods

To this end, we applied a qualitative and case study approach, interviewing 13 physicians in Portugal, including general practitioners and specialists. The data was collected using a semi-structured interview script, and a content analysis was performed to categorize the responses and gain a comprehensive understanding of the phenomenon.

Results

The main results of this study highlight the need for a more systematic approach to training physicians, including the relationship between climate change and health. Concerning policies, this research highlights the need for more consistent communication and precise guidelines for dealing with the impacts of climate change on public health.

Conclusion

As the first exploratory study focusing on Portuguese physicians, this research provides unique insights into their views on the potential to influence patient behavior and health policy. Importantly, it offers valuable recommendations for health policy strategies, particularly in awareness-raising and training plans for these professionals, thereby demonstrating the research’s practical implications.
Humanising processes after harm part 2: compounded harm experienced by patients and their families after safety incidents
Authors: Ramsey Lauren , Hughes Joanne , Hazeldine Debra , Seddon Sarah , Gould Mary , Wailling Jo , Murray Jenni , McHugh Siobhan , Simms-Ellis Ruth , Halligan Daisy , Ludwin Katherine , O’Hara Jane K.
Published: December 2024

Background

Healthcare organisations risk harming patients and their families twofold. First, through the physical, emotional and/or financial harm caused by safety incidents themselves, and second, through the organisational response to incidents. The former is well-researched and targeted by interventions. However, the latter, termed ‘compounded harm’ is rarely acknowledged.

Aims

We aimed to explore the ways compounded harm is experienced by patients and their families as a result of organisational responses to safety incidents and propose how this may be reduced in practice.

Methods

We used framework analysis to qualitatively explore data derived from interviews with 42 people with lived or professional experience of safety incident responses. This comprised 18 patients/relatives, 16 investigators, seven healthcare staff and one legal staff. People with lived and professional experience also helped to shape the design, conduct and findings of this study.

Findings

We identified six ways that patients and their families experienced compounded harm because of incident responses. These were feeling: (1) powerless, (2) inconsequential, (3) manipulated, (4) abandoned, (5) de-humanised and (6) disoriented.

Discussion

It is imperative to reduce compounded harm experienced by patients and families. We propose three recommendations for policy and practice: (1) the healthcare system to recognise and address epistemic injustice and equitably support people to be equal partners throughout investigations and subsequent learning to reduce the likelihood of patients and families feeling powerless and inconsequential; (2) honest and transparent regulatory and organisational cultures to be fostered and enacted to reduce the likelihood of patients and families feeling manipulated; and (3) the healthcare system to reorient towards providing restorative responses to harm which are human centred, relational and underpinned by dignity, safety and voluntariness to reduce the likelihood of patients and families feeling abandoned, de-humanised and disoriented.
Launching Stealth AutoGuideTM robot for stereotactic biopsy procedures in a neurosurgical centre: learning curve and workflow optimization
Authors: Barth Marcus , Holl Etienne , Flaschka Fabian , Karakaya Sila , Körbler Vitus , Pichlsberger Melanie , Wolfsberger Stefan , Micko Alexander
Published: December 2024

Background

Accurate histological analysis is crucial for confirming intracerebral neoplasia due to the diverse array of potential diagnoses presented by imaging. In the realm of biopsy techniques, the use of robot-based systems is on the rise, primarily owing to their heightened targeting accuracy. The objective of this study was to elucidate the practicality, learning curve and workflow associated with robot-guided biopsies upon their introduction to a neurosurgical centre.

Materials and methods

In March 2022, the neurosurgical department at our institution adopted the Medtronic Stealth AutoGuide™ cranial robotic guidance platform, a miniaturized robotic guidance device designed for stereotactic procedures. Four experienced neurosurgeons underwent training with the Stealth AutoGuide™ system, after which 51 consecutive biopsies were performed. The evaluation encompassed entry and target accuracy, preoperative setup time, time required for the biopsy procedure, and overall operating time. Statistical analysis was conducted to identify any differences between the initial 26 and subsequent sets of 25 patients, with the aim of identifying changes in workflow.

Results

The study included all patients necessitating a diagnostic biopsy for intracerebral tumours, with only one patient excluded due to the inaccessibility of the intended target point caused by tumour calcification. Notably, there were no significant differences between the first 25 and last 26 patients in the median time from incision to the first biopsy (overall: 11.5 min, IQR 9.03–15.0), the procedure time (overall: 30.0 min, IQR 23.5–46.5), median accuracy at entry (overall: 2.05 mm, IQR 0.8–3.8), or target point (overall: 2.2 mm (IQR 1.6–3.7). However, a significant reduction in robot setup time was observed between the two groups, median 2.69 min versus 1.17 min, respectively (p ≤ 0.001).

Conclusion

The deployment of the robotic biopsy system, Stealth AutoGuide™, showed high feasibility, steep learning curve due to uncomplicated technical handling using our standardized operative workflow. Therefore, even in prone position a high diagnostic yield was achieved. Overall, the Stealth AutoGuide™ system facilitated biopsies in traditionally challenging regions with concise procedure time and surgeon-independent high accuracy.
Die Theorie der syntaktischen Gruppen (J. Fourquet): eine neue grammatische Terminologie
Authors: Robin Thérèse
Published: December 2024

The paper aims to present the specificity of Fourquet’s syntactic group theory and to show how it fi ts into his didactic and pedagogical approaches as well as his linguiti c research in general. Fourquet challenges traditional grammar, the French approach to the German language system, and emphasises the need for a new perspective on language teaching, by moving away from the concept of word towards the concept of group. Syntax is also linked to semantics and even pragmatics. This is Fourquet’s undeniable contribution, albeit it is largely ignored today. The article also explains to what extent Fourquet’s group theory is innovative and discusses the current impact of the theory on German teaching in France. Fourquet’s works in French and German are also be analysed from this perspective.
TacFR-Gripper: A Reconfigurable Fin-Ray-Based Gripper with Tactile Skin for In-Hand Manipulation
Authors: Cong Qingzheng , Fan Wen , Zhang Dandan
Published: December 2024

This paper introduces the TacFR-Gripper, a novel reconfigurable soft robotic gripper inspired by the Fin-Ray effect and equipped with tactile skin. The gripper incorporates a four-bar mechanism for accurate finger bending and a reconfigurable design to change the relative positions between the fingers and palm, enabling precise and adaptable object grasping. This 5-Degree-of-Freedom (DOF) soft gripper can facilitate dexterous manipulation of objects with diverse shapes and stiffness and is beneficial to the safe and efficient grasping of delicate objects. An array of Force Sensitive Resistor (FSR) sensors is embedded within each robotic fingertip to serve as the tactile skin, enabling the robot to perceive contact information during manipulation. Moreover, we implemented a threshold-based tactile perception approach to enable reliable grasping without accidental slip or excessive force. To verify the effectiveness of the TacFR-Gripper, we provide detailed workspace analysis to evaluate its grasping performance and conducted three experiments, including (i) assessing the grasp success rate across various everyday objects through different finger configurations, (ii) verifying the effectiveness of tactile skin with different control strategies in grasping, and (iii) evaluating the in-hand manipulation capabilities through object pose control. The experimental results indicate that the TacFR-Gripper can grasp a wide range of complex-shaped objects with a high success rate and deliver dexterous in-hand manipulation. Additionally, the integration of tactile skin is demonstrated to enhance grasp stability by incorporating tactile feedback during manipulations.
Does Green Finance Enhance the Ecological Value Level of Cultivated Land? Evidence from Mainland China
Authors: Pei Ben , Chen Shulin
Published: December 2024

The ecological value of cultivated land contributes to a harmonious agricultural environment. Green finance support is essential to promoting the ecological value of cultivated land. Nevertheless, research specifically centered on cultivated land as a primary focus remains limited in exploring the relationship between ecological value and green finance, ignoring the fact that green finance can bolster ecological functions and drive sustainable practices. To address this gap, an objective indicator of the cultivated land ecological value level was introduced, and its variations at both the provincial and national levels from 2003 to 2022 were investigated. Results indicate that the cultivated land ecological value level increased over time, with higher values clustering spatially in southern regions. Subsequently, specific spatial correlations between the cultivated land ecological value level and green finance support were revealed using a spatial Durbin model. The results show that green finance support enhanced the cultivated land ecological value level, with its spatial lag term being particularly pronounced. These correlations were evident in eastern regions but were insignificant in western regions. Furthermore, a detailed range of spatial indirect spillover effects was estimated, demonstrating that the spatial effects on other provinces were positive when the geographic distance between them was close. In summary, these conclusions offer practical recommendations for the eco-friendly management of cultivated land, including strategies for vertical collaboration between central and local administrations and horizontal adaptation by governments in the east, central, and west regions based on local conditions.
Comparative Salt-Stress Responses in Salt-Tolerant (Vikinga) and Salt-Sensitive (Regalona) Quinoa Varieties. Physiological, Anatomical and Biochemical Perspectives
Authors: Serrat Xavier , Quello Antony , Manikan Brigen , Lino Gladys , Nogués Salvador
Published: December 2024

Soil salinization is an important stress factor that limits plant growth and yield. Increased salinization is projected to affect more than 50% of all arable land by 2050. In addition, the growing demand for food, together with the increase in the world population, forces the need to seek salt-tolerant crops. Quinoa (Chenopodium quinoa Willd.) is an Andean crop of high importance, due to its nutritional characteristics and high tolerance to different abiotic stresses. The aim of this work is to determine the physiological, anatomical, and biochemical salt-tolerance mechanisms of a salt-tolerant (Vikinga) and a salt-sensitive (Regalona) quinoa variety. Plants were subjected to salinity stress for 15 days, starting at 100 mM NaCl until progressively reaching 400 mM NaCl. Physiological, anatomical, and biochemical parameters including growth, chlorophyll content, quantum yield of PSII (ϕPSII), gas exchange, stomatal density, size, and lipid peroxidation (via malondialdehyde, MDA) were measured. Results show that chlorophyll content, ϕPSII, and MDA were not significantly reduced under saline stress in both varieties. The most stress-affected process was the CO2 net assimilation, with an up to 60% reduction in both varieties, yet Vikinga produced higher dry weight than Regalona due to the number of leaves. The stomatal densities increased under salinity for both varieties, with Regalona the one showing higher values. The averaged stomatal size was also reduced under salinity in both varieties. The capacity of Vikinga to generate higher dry weight is a function of the capacity to generate greater amounts of leaves and roots in any condition. The stomatal control is a key mechanism in quinoa’s salinity tolerance, acquiring higher densities with smaller sizes for efficient management of water loss and carbon assimilation. These findings highlight the potential of Vikinga for cultivation in temperate salinized environments during winter, such as Deltas and lowlands where rice is grown during summer.
Exploring Feed Efficiency in Beef Cattle: From Data Collection to Genetic and Nutritional Modeling
Authors: Ojo Ayooluwa O. , Mulim Henrique A. , Campos Gabriel S. , Junqueira Vinícius Silva , Lemenager Ronald P. , Schoonmaker Jon Patrick , Oliveira Hinayah Rojas
Published: December 2024

Increasing feed efficiency in beef cattle is critical for meeting the growing global demand for beef while managing rising feed costs and environmental impacts. Challenges in recording feed intake and combining genomic and nutritional models hinder improvements in feed efficiency for sustainable beef production. This review examines the progression from traditional data collection methods to modern genetic and nutritional approaches that enhance feed efficiency. We first discuss the technological advancements that allow precise measurement of individual feed intake and efficiency, providing valuable insights for research and industry. The role of genomic selection in identifying and breeding feed-efficient animals is then explored, emphasizing the benefits of combining data from multiple populations to enhance genomic prediction accuracy. Additionally, the paper highlights the importance of nutritional models that could be used synergistically with genomic selection. Together, these tools allow for optimized feed management in diverse production systems. Combining these approaches also provides a roadmap for reducing input costs and promoting a more sustainable beef industry.
The Prevalence of Gastric Ulcer Syndrome in 395 Horses in Jiangyin City, China, Jiangsu Province
Authors: Zhou Kairen , Dong Zhen , Zhou Xuzheng , Zhai Bintao , Li Bing , Zhang Jiyu , Cheng Fusheng
Published: December 2024

The aim of this study was to determine the prevalence and association of EGUS in horses of different ages, breeds and occupations. Gastroscopies were performed on 395 horses, and gastric ulcers were graded on a scoring system from 0 to 4. The relationship between age, breed, and work, along with the prevalence of gastric ulcers and their influences, were evaluated. The prevalence rate of ulcers in this herd was 78%. Older horses were prone to ulcers in both the glandular and squamous mucosal areas. Across the different jobs surveyed, 60% of the horses had a score of 2 or above. For the horses participating in more intense jobs (group performances, pulling carts, etc.), the number of horses with an ulcer score of 2 or above exceeded 50% of the total number of horses in this job. The prevalence of gastric ulcers was high, and these ulcers were severe in the horses belonging to the examined club, with no association between age or breed and the prevalence of ulcers; however, there was a difference in the location of the ulcers between breeds, as well as differences in the incidence and severity of ulcers depending on work activity. This study provides data reference values for the control and prevention of gastric ulcers in horses in this horse farm.
Endocrine Mucin-Producing Sweat Gland Carcinoma (EMPSGC) in a Dog: Immunohistochemical Characterization
Authors: Tangchang Warisraporn , Jung Gi-young , Song Jun-yeop , Kumbukgahadeniya Poornima , Kim Dae-hyun , Kwon Hyo-jung , Son Hwa-young
Published: December 2024

A seven-year-old spayed female dog presented with multiple, small oval-shaped masses on the abdominal skin. The excised tissue was submitted for histopathological evaluation. Routine histology revealed basaloid nodules with stippled chromatin and pleomorphic nuclei. Morphologically, the tumor resembled a solid papillary carcinoma of the breast or an endocrine mucin-producing sweat gland carcinoma (EMPSGC). EMPSGC is a rare neuroendocrine-differentiated neoplasm. This study aimed to compare different immunohistochemical markers for distinguishing between canine sweat gland carcinoma (SGC) and mammary gland carcinoma (MGC). CK19 and Sox9 were useful in identifying SGC. Additionally, the tumor was positive for CK5, AE1/AE3+CK8/18, p63, vimentin, E-cadherin, and synaptophysin, while estrogen receptor staining was negative. The final diagnosis of canine EMPSGC was based on the tumor’s morphological and neuroendocrine immunohistopathological features. Our report provides the first detailed description of a canine EMPSGC, an exceedingly rare tumor in dogs.
Does a Specific Sequential Combination of Antiseptic Solutions for Chemical Debridement in Periprosthetic Joint Infection Improve Outcomes vs. Solution Alone? An In Vivo Study
Authors: Márquez-Gómez Miguel , Díaz-Navarro Marta , Visedo Andrés , Prats-Peinado Lourdes , Muñoz Patricia , Vaquero Javier , Guembe María , Sanz-Ruíz Pablo
Published: December 2024

Background: Chemical debridement is a fundamental step during the surgical treatment of both acute and chronic periprosthetic joint infection (PJI). However, there is no consensus on the optimal solution, nor is there sufficient evidence on the optimal irrigation time and combination of solutions. In an in vitro study, our group recently demonstrated that sequential combination debridement (SCD) with 3% acetic acid (AA) followed by 10% povidone iodine (PI) and 5 mM hydrogen peroxide (H2O2) was the best strategy for reducing bacterial load. The present study aimed to validate these findings in an in vivo model. Results: The median (IQR) log CFU/mL was lower in the group of mice treated with SCD (2.85 [0.00–3.72]) than in the Bactisure™ group (4.02 [3.41–4.72], p = 0.02). While this reduction was also greater than in the PI group (3.99 [1.11–4.33]), the difference did not reach statistical significance (p = 0.19). Cell viability assays showed no differences between treatments. S. aureus bacteremia was detected in 10% of mice treated with SCD, compared to 30% in the PI group and 10% in the Bactisure™ group. The difference was not statistically significant (p = 0.36). Conclusion: Our findings confirm that SCD significantly reduced bacterial load in an in vivo S. aureus PJI model, showing superior anti-biofilm activity compared to Bactisure™ and comparable performance to PI alone. These results highlight SCD’s potential to serve as a standardized chemical debridement protocol, combining enhanced efficacy with clinical applicability. Methods: We tested SCD with 3% AA for 3 min, 10% PI for 3 min, and H2O2 for 3 min in a 7-day Staphylococcus aureus (ATCC29213)-based murine femur PJI model and compared the results with single treatments of 10% PI for 3 min or Bactisure™ solution for 3 min. A sterile steel implant with local administration of saline solution for 3 min was used as a non-infected control. After completing irrigation procedures, under anesthesia, mice were euthanized, and implants were analyzed for CFU/mL counts and cell viability rates. Blood cultures were obtained pre-euthanasia to detect bacteremia.
Chemical Composition and Biological Activities of St John’s Wort (Hypericum perforatum L.) Essential Oil from Bulgaria
Authors: Tumbarski Yulian , Ivanov Ivan , Todorova Mina , Gerasimova Anelia , Dincheva Ivayla , Makedonski Lubomir , Nikolova Krastena
Published: December 2024

Since ancient times, essential oils obtained from various aromatic plants have been utilized as bioactive ingredients in medicines, foods and cosmetics. The present study aimed to investigate the chemical composition and biological activities of St John’s Wort (Hypericum perforatum L.) essential oil (SJW EO) from Bulgaria, which is known to possess various biological properties. Gas chromatography and mass spectrometry (GC–MS) analysis, determination of antioxidant activity (by the ABTS method), an antimicrobial activity test and an in vitro anti-inflammatory activity test were performed. The main classes of compounds identified by GC–MS analysis were monoterpenes (43.55%), followed by sesquiterpenes (36.81%) and alkanes (16.92%). The predominant chemical components of SJW EO were α-pinene (27.52%), followed by β-pinene (10.08%), β-caryophyllene (6.77%), germacrene D (6.37%) and caryophyllene oxide (4.48%). The highest antibacterial activity was observed against the Gram-negative bacteria Klebsiella pneumoniae ATCC 13883 (inhibition zone of 12.0 mm) and Pseudomonas aeruginosa ATCC 9027 (inhibition zone of 11.0 mm). SJW EO exhibited significant in vitro anti-inflammatory activity, as the results demonstrated that its anti-inflammatory effect was stronger than those of the conventional anti-inflammatory drugs Prednisolon Cortico and acetylsalicylic acid (Aspirin), which were used as controls (all in concentration of 1 mg/mL). The obtained results demonstrated that Bulgarian SJW EO can be used as an active ingredient in the composition of new products for the pharmaceutical and cosmetic industries.
Estimating Tetrachloroethene Sorption Coefficients Based on Soil Properties in Organic-Poor Soils
Authors: Rippelová Veronika , McGachy Lenka , Janků Josef , Kroužek Jiří
Published: December 2024

In the context of contaminated site remediation, the fate of chlorinated solvents in the subsurface and subsequent groundwater contamination is influenced by soil properties governing sorption. The solid–water distribution coefficient (Kd) is a key parameter for modeling contaminant distribution and transport, essential for risk assessment and remediation planning. This study evaluated tetrachloroethene sorption isotherms in 34 low-organic-carbon soils from the Czech Republic, assessing the influence of soil properties on Kd. Soil samples exhibited variability in organic carbon content (˂0.05–0.81%), with clay ranging from 0% to 64.9%, silt 5.1% to 71.2%, and sand 5.2% to 88.9%, specific surface area (0.41–64.39 m2 g−1), particle density (2.05–4.09 g cm−3), and porosity (43.5–67.3%). Batch experiments were conducted using standard procedures, with Kd values ranging from 0.379 to 2.272 L kg−1. Statistical analysis grouped the soils into three textural classes: sandy, clayey fine, and silty loam. The findings reveal that organic carbon content and specific surface area are the primary predictors of Kd, while clay and sand also play a significant role in shaping sorption behavior. Multivariate regression models explained 63.6% to 98.5% of Kd variability with high accuracy, as indicated by low root means square error (0.070–0.329) and mean absolute percentage error (3.8–28.8%) values. These models offer reliable predictions of sorption behavior, providing valuable tools for risk assessment and remediation strategies.