bluuebunny/crossref_metadata_2025 huggingface dataset:
The Methylation and Expression of LINC00511, an Important Angiogenesis-Related lncRNA in Stomach Adenocarcinoma
Authors: Li Zhiying , Chen Yingli , Zhao Yuanyuan , Li Qianzhong
Published: February 2025

Stomach adenocarcinoma (STAD) has high incidence and mortality rates. Long non-coding RNAs (lncRNAs) and angiogenesis are closely related to the pathogenesis and metastasis of STAD. Recently, emerging evidence demonstrated that DNA methylation plays crucial roles in the development of STAD. This study explored the relationship between DNA methylation and the abnormal expression of angiogenesis-related lncRNAs (ARlncRNAs) in stomach adenocarcinoma, aiming to identify prognostic biomarkers. Moreover, a Cox analysis and Lasso regression were used to establish an ARlncRNA feature set related to angiogenesis. The prognostic model was evaluated by using a Kaplan–Meier (KM) analysis, ROC curves, and nomograms. Based on the identified 18 key ARlncRNAs, a prognostic predictive model was constructed. In addition, a specific ARlncRNA with abnormal methylation in the model, LINC00511, showed significant differences in expression and methylation across different subgroups. The methylation and expression of LINC00511 were analyzed by a correlation and co-expression analysis. The correlation analysis indicated that promoter methylation may improve LINC00511 expression. Further analysis found 355 mRNAs co-expressed with LINC00511 which may interact with 6 miRNAs to regulate target gene expression. The abnormal methylation of LINC00511 could significantly contribute to the progression of stomach adenocarcinoma.
Phosphatidylcholine Cytidine Transferase α (CCTα) Affects LD Formation Through Fusion and Lipophagy in Bovine Mammary Epithelial Cells
Authors: Yang Jingna , Fan Yuxin , Kang Fangyuan , Yang Yanbin , Wang Yueying , Liu Yang , Han Liqiang
Published: February 2025

Phosphatidylcholine cytidine transferase α (CCTα) is a key rate-limiting enzyme in the CDP–choline pathway, the primary pathway for phosphatidylcholine (PC) synthesis in mammals. This study investigated the role of CCTα in lipid droplet (LD) formation, phospholipid synthesis, LD fusion, and lipophagy in bovine mammary epithelial cells (BMECs) through CCTα gene knockout (CCT-KO) and overexpression (CCT-OE). CCTα mRNA expression was significantly increased in bovine mammary gland tissue after lactation. In BMECs, CCTα was transferred from the nucleus to the endoplasmic reticulum and localized on LD surfaces in the presence of linoleic acid. Compared with normal BMECs (NC), CCTα knockout (CCT-KO) cells had significantly greater LD diameters (1.53 μm vs. 1.68 μm, p < 0.05), lower proportions of small LDs (<1 µm; 11.39% vs. 5.42%), and higher proportions of large LDs (>3 µm; 0.67% vs. 2.88%). In contrast, CCTα overexpression (CCT-OE) decreased the diameter of LDs to 1.18 μm (p < 0.01), increased the proportion of small LDs to 35.48%, and decreased the proportion of large LDs to 0.24%. CCTα knockout significantly decreased the PC content and the ratio of PC to PE, whereas CCTα overexpression increased the PC content and the ratio of PC to phosphatidyl ethanolamine (PE) (p < 0.05). The lipidomics analysis indicated that PC synthesis was significantly influenced by CCTα gene expression. Live cell observations showed that CCTα knockout promoted the fusion of small LDs into large LDs. In cells with CCT α overexpression, the expression of the microtubule-associated protein 1 light chain 3 (LC3) protein and the number of lysosomes was elevated, and the lysosomal phagocytosis of LDs was observed through transmission electron microscopy, thus indicating that CCTα overexpression enhanced lipophagy. In conclusion, these results suggest that CCTα plays a role in regulating LD formation by influencing PC synthesis, LD fusion, and lipophagy in BMECs.
Design and Synthesis of New 5-Methylisatin Derivatives as Potential CDK2 Inhibitors
Authors: Czeleń Przemysław , Skotnicka Agnieszka , Szefler Beata , Kabatc-Borcz Janina , Sutkowy Paweł
Published: February 2025

Cancer remains one of the leading causes of death globally, driving the need for effective therapies. Targeting cyclin-dependent kinase 2 (CDK2), a critical cell cycle regulator, is a promising approach for cancer treatment. This study developed a new group of 5-methylisatin derivatives with strong binding potential to CDK2. By combining the isatin core with various benzoylhydrazide substituents, the design process was guided by molecular docking, dynamic simulations, and ADMET analysis. Thirty-one derivatives were modelled, and a subset was synthesised and characterised for their physicochemical and spectroscopic properties. The analysis suggested that substitutions at R2 and R3 positions improved binding affinity, while modifications at R4 were less favourable. Hydrogen bonds with GLU81 and LEU83, along with hydrophobic interactions, were key to stabilising the complexes. A comparison with a reference molecule (RM) 3-((2,6-Dichlorobenzylidene)hydrazono)indolin-2-one, showing inhibitory activity similar to doxorubicin, revealed several advantages for the new derivatives. The multidimensional comparative analysis highlighted significant improvements in active site affinity, conformational stability, and fit. ADMET analysis confirmed comparable performance in most areas, with superior bioavailability observed in derivatives 1, 2a, 2b, 3h, 3b, and 3e. These results suggest that 5-methylisatin derivatives could be promising CDK2 inhibitors.
Exploring Nano-Delivery Systems to Enhance the Edaravone Performance in Amyotrophic Lateral Sclerosis Treatment
Authors: Aguiar Brandon , Alfenim Ana Rita , Machado Cláudia Sofia , Moreira Joana , Pinto Miguel , Otero-Espinar Francisco J. , Borges Fernanda , Fernandes Carlos
Published: February 2025

Edaravone is one of the treatment options for Amyotrophic Lateral Sclerosis, but its therapeutic efficacy is limited due to the incapacity to cross the blood–brain barrier, as well as its short life span and poor stability, which is ultimately caused by its tautomerism in physiological condions. This work presents an overview about the use of several nanoformulations based on polymeric, protein, lipidic, or hybrid structure as suitable and stable drug delivery systems for encapsulating edaravone. We also evaluated the functionalization of nanoparticles with pegylated chains using the polyethylene glycol or tocopherol polyethylene glycol succinate and the possibility of preparing polymeric nanoparticles at different pH (7.4, 9, and 11). Edaravone was sucessfully encapsulated in polymeric, lipid–polymer hybrid, and lipidic nanoparticles. The use of higher pH values in the synthesis of polymeric nanoparticles has led to a decrease in nanoparticle size and an increase in the percentage of encapsulation efficiency. However, the resulting nanoformulations are not stable. Only polymeric and hybrid nanoparticles showed good stability over 80 days of storage, mainly at 4 °C. Overall, the nanoformulations tested did not show cytotoxicity in the SH-SY5Y cell line except the nanostructured lipid carrier formulations that showed some cytotoxicity possibly due to lipidic peroxidation. In conclusion, this work shows that edaravone can be encapsulated in different nanocarriers that could act as an interesting alternative for the treatment of Amyotrophic Lateral Sclerosis.
Vitamin D and Type 2 Diabetes Mellitus: Molecular Mechanisms and Clinical Implications—A Narrative Review
Authors: Fuentes-Barría Héctor , Aguilera-Eguía Raúl , Flores-Fernández Cherie , Angarita-Davila Lissé , Rojas-Gómez Diana , Alarcón-Rivera Miguel , López-Soto Olga , Maureira-Sánchez Juan
Published: February 2025

Vitamin D has been widely studied for its implications on type 2 diabetes mellitus, a chronic condition characterized by insulin resistance, inflammation, and metabolic dysfunction. This review explores the molecular mechanisms underpinning vitamin D’s effects on glucose metabolism, inflammation, and adipogenesis, while assessing its potential clinical applications in type 2 diabetes. In its 1,25-dihydroxyvitamin D3 form, vitamin D modulates various metabolic processes, affecting proinflammatory cytokines and activating the AMPK pathway, inhibiting mTOR signaling, and promoting adipocyte differentiation. These effects enhance insulin sensitivity and reduce chronic inflammation, key contributors to metabolic dysfunction. In this context, the progression of prediabetes has been linked to vitamin D, which limits pathological progression and increases the likelihood of restoring a normal metabolic state, crucial in diabetes progression. Moreover, vitamin D has been reported to reduce the likelihood of developing diabetes by 15%, particularly in doses higher than the traditional recommendations for bone health. Despite promising evidence, discrepancies in study designs, serum vitamin D measurements, and population-specific factors highlight the need for standardized methodologies and personalized approaches. In conclusion, vitamin D has complementary therapeutic potential in treating type 2 diabetes, revealing gaps in research, such as optimal dosing and long-term effects across populations. Future studies should integrate molecular insights into clinical practice to optimize vitamin D’s impact on metabolic health.
Impact of Saharan Dust and SERPINA1 Gene Variants on Bacterial/Fungal Balance in Asthma Patients
Authors: Escuela-Escobar Ainhoa , Perez-Garcia Javier , Martín-González Elena , González Martín Cristina , Hernández-Pérez José M. , González Pérez Ruperto , Sánchez Machín Inmaculada , Poza Guedes Paloma , Mederos-Luis Elena , Pino-Yanes María , Lorenzo-Díaz Fabian , González Carracedo Mario A. , Pérez Pérez José A.
Published: February 2025

The Canary Islands, a region with high asthma prevalence, are frequently exposed to Saharan Dust Intrusions (SDIs), as are a wide range of countries in Europe. Alpha-1 antitrypsin (SERPINA1 gene) regulates the airway’s inflammatory response. This study analyzed the combined effect of SDI exposure and SERPINA1 variants on bacterial/fungal DNA concentrations in saliva and pharyngeal samples from asthmatic patients. Bacterial and fungal DNAs were quantified by qPCR in 211 asthmatic patients (GEMAS study), grouped based on their exposure to daily PM10 concentrations. Associations between SDI exposure, microbial DNA concentrations, and nine variants in SERPINA1 were tested using linear regression models adjusted for confounders. The ratio between bacterial and fungal DNA was similar in saliva and pharyngeal samples. SDI exposure for 1–3 days was enough to observe significant microbial DNA change. Increased bacterial DNA concentration was detected when SDI exposure occurred 4–10 days prior to sampling, while exposure between days 1 and 3 led to a reduction in the fungal DNA concentration. The T-allele of SERPINA1 SNV rs2854254 prevented the increase in the bacterial/fungal DNA ratio in pharyngeal samples after SDI exposure. The bacterial/fungal DNA ratio represents a potential tool to monitor changes in the microbiome of asthmatic patients.
Structure–Function Analysis of the Self-Sufficient CYP102 Family Provides New Insights into Their Biochemistry
Authors: Padayachee Tiara , Lamb David C. , Nelson David R. , Syed Khajamohiddin
Published: February 2025

Cytochromes P450 are a superfamily of heme-containing monooxygenases involved in a variety of oxidative metabolic reactions, primarily catalyzing the insertion of an oxygen atom into a C-H bond. CYP102 represents the first example of a bacterial P450 that can be classified as a type II (eukaryotic-like) P450 and functions as a catalytically self-sufficient enzyme. These unique features have made CYP102 an attractive system for studying P450 structure and function. However, an overall picture of the specific amino acid residues that are crucial to the functioning of CYP102 and the effect of mutations on the P450 structure and catalysis is yet to be reported. Such an approach will aid protein engineering approaches used to improve this enzyme. To address this research knowledge gap, we have investigated 105 CYP102 crystal structures in this study. We demonstrate that the CYP102 active site is highly dynamic and flexible. Amino acid residues that play critical roles in substrate binding, orientation, and anchoring were identified. Mutational studies highlighted the roles of amino acids and provided possible bioengineering improvement strategies for CYP102. Decoy molecules are a promising agent for deceiving CYP102 and permitting non-native substrates into the active site. Ru(II)-diimine photosensitizers and zinc/cobalt (III) sepulchrate (Co(III)Sep) could be used as alternative electron sources. The present study serves as a reference for understanding the structure–functional analysis of CYP102 family members precisely and of P450 enzymes in general. Significantly, this work contributes to the effort to develop an improved CYP102 enzyme, thereby advancing the field of P450 research and potentially leading to new industrial applications.
A Novel 6-bp Repeat Unit (6-bp RU) of the 13th Intron Within the Conserved EPAS1 Gene in Plateau Pika Is Capable of Altering Enhancer Activity
Authors: Tang Qi , Xu Yuhui , Song Qingchuan , Cao Siqi , Li Yang , Lan Xianyong , Zhang Liangzhi , Pan Chuanying
Published: February 2025

The plateau pika (pl-pika), a resilient mammal of the Qinghai-Tibet Plateau, exhibits remarkable adaptations to extreme conditions. This study delves into mutations within the Endothelial PAS Domain Protein 1 (EPAS1) gene, crucial for high-altitude survival. Surprisingly, a novel 6-bp insertion/deletion (indel) mutation in EPAS1’s Intron 13, along with an additional repeat unit downstream, was discovered during PCR amplification. Genetic analysis across altitude gradients revealed a correlation between this indel’s frequency and altitude, hinting at its role in altitude adaptation. Fluorescence enzyme assays unveiled enhancer activity within Intron 13, where the deletion of repeat units led to increased activity, indicating potential transcription factor binding. Notably, GCM1 emerged as a candidate transcription factor binding to the indel site, suggesting its involvement in EPAS1 regulation. These findings enrich our comprehension of high-altitude adaptation in plateau pikas, shedding light on the intricate interplay between genetic mutations, transcriptional regulation, and environmental pressures in evolutionary biology.
Special Issue “Cancer Biomarker: Current Status and Future Perspectives”
Authors: Costantini Susan
Published: February 2025

In recent years, advancements in omics technologies have significantly accelerated the identification of a broad spectrum of biomarkers based on DNA, RNA, microRNAs (miRNAs), and long non-coding RNAs, as well as proteins and metabolic and lipid alterations (Figure 1) [...]
Unusual Partners: γδ-TCR-Based T Cell Therapy in Combination with Oncolytic Virus Treatment for Diffuse Midline Gliomas
Authors: Vazaios Konstantinos , Hernández López Patricia , Aarts-Riemens Tineke , Daudeij Annet , Kemp Vera , Hoeben Rob C. , Straetemans Trudy , Hulleman Esther , Calkoen Friso G. , van der Lugt Jasper , Kuball Jürgen
Published: February 2025

Due to the minimal survival benefits of existing therapies for pediatric diffuse midline glioma (DMG) patients, new therapeutic modalities are being investigated. Immunotherapies such as CAR-T cells and oncolytic viruses (OVs) are part of these efforts, as evidenced by the increasing number of clinical trials. αβ T cells engineered with a high-affinity γ9δ2 T-cell receptor (TEGs) are immune cells designed to target metabolic changes in malignant or virally infected cells via BTN2A1 and BTN3A. Because the expression of BTN2A1 and BTN3A can be altered in tumor and infected cells, combining TEGs and OVs could potentially enhance the anti-tumor response. We investigated this hypothesis in the following study. We demonstrate that TEGs can indeed target DMG, which expresses BTN2A1 and BTN3A at varying levels, and that OVs can further enhance the expression of BTN3A—but not BTN2A1—in DMG. Functionally, TEGs killed DMG cell cultures, and this killing was further increased after OV infection of the DMGs with either adenovirus Δ24-RGD or reovirus R124 under suboptimal conditions. However, this additive effect was lost when γ9δ2 TCR–ligand interaction was boosted by pamidronate. This study demonstrates the additive effect of combining OVs and Vγ9Vδ2 TCR-engineered immune cells under suboptimal conditions and supports a combination strategy to enhance the efficacy of both therapeutic modalities.
Genome-Wide Identification and Expression Analysis of the TGA Gene Family in Banana (Musa nana Lour.) Under Various Nitrogen Conditions
Authors: Zhang Bencheng , Wang Wei , Wang Can , Cai Bingyu , Feng Junting , Zhou Dengbo , Chen Yufeng , Zhang Miaoyi , Qi Dengfeng , Wang Zhuo , Wei Yongzan , Xie Jianghui
Published: February 2025

The TGA (TGACG motif-binding factor) transcription factors are integral to root growth and development, and are pivotal in mediating plant responses to abiotic stresses. Nonetheless, their role in the nutrient absorption processes of banana plants has not been extensively investigated. This research conducted a comprehensive analysis of the MaTGA gene family, emphasizing their physicochemical characteristics, phylogenetic relationships, gene duplication events, promoter cis-regulatory elements and protein interaction networks. Furthermore, this study investigated the expression patterns of MaTGA family members under varying nitrogen conditions. A total of 18 MaTGA members were identified within the banana genome, each encoding proteins characterized by the presence of bZIP and DOG domains. These genes exhibited an uneven distribution across eight chromosomes. Phylogenetic analysis further classified the MaTGA family into four distinct subgroups (I–IV), consisting of three, seven, three, and five members, respectively. An analysis of promoter cis-elements indicated that over 50% of the MaTGA gene family members contain hormone-responsive elements associated with abscisic acid (ABRE), ethylene (ERE), and salicylic acid (SARE), in addition to stress-responsive elements related to drought (MBS) and low temperature (LTR). Regarding gene expression, MaTGA7, MaTGA8, and MaTGA15 exhibited significantly elevated expression levels in the leaves and roots relative to other tissues. Under varying nitrogen conditions, 13 members, including MaTGA7 and MaTGA8, demonstrated the highest expression levels under reduced nitrogen (70%) treatment, followed by low nitrogen (20%) conditions, and the lowest expression levels were observed under nitrogen-deficient conditions. These findings imply that MaTGA genes may play crucial roles in enhancing nitrogen use efficiency. Protein interaction predictions suggest that MaTGA7, MaTGA8, and MaTGA15 may interact with nitrogen-related proteins, including Nitrate Transporter 2 (NRT2.1 and NRT2.2), NIN-Like Protein 7 (NLP7), and Nitrate Transporter 1.1 (NPF6.3). In summary, MaTGA7, MaTGA8, and MaTGA15 are likely involved in the processes of nitrogen absorption and utilization in bananas. The present findings establish a basis for subsequent investigations into the functional roles of MaTGA genes in augmenting nutrient use efficiency and mediating responses to abiotic stresses in banana plants.
Micronutrient Biofortification in Wheat: QTLs, Candidate Genes and Molecular Mechanism
Authors: Nasim Adnan , Hao Junwei , Tawab Faiza , Jin Ci , Zhu Jiamin , Luo Shuang , Nie Xiaojun
Published: February 2025

Micronutrient deficiency (hidden hunger) is one of the serious health problems globally, often due to diets dominated by staple foods. Genetic biofortification of a staple like wheat has surfaced as a promising, cost-efficient, and sustainable strategy. Significant genetic diversity exists in wheat and its wild relatives, but the nutritional profile in commercial wheat varieties has inadvertently declined over time, striving for better yield and disease resistance. Substantial efforts have been made to biofortify wheat using conventional and molecular breeding. QTL and genome-wide association studies were conducted, and some of the identified QTLs/marker-trait association (MTAs) for grain micronutrients like Fe have been exploited by MAS. The genetic mechanisms of micronutrient uptake, transport, and storage have also been investigated. Although wheat biofortified varieties are now commercially cultivated in selected regions worldwide, further improvements are needed. This review provides an overview of wheat biofortification, covering breeding efforts, nutritional evaluation methods, nutrient assimilation and bioavailability, and microbial involvement in wheat grain enrichment. Emerging technologies such as non-destructive hyperspectral imaging (HSI)/red, green, and blue (RGB) phenotyping; multi-omics integration; CRISPR-Cas9 alongside genomic selection; and microbial genetics hold promise for advancing biofortification.
Systematic Review of Phylogenetic Analysis Techniques for RNA Viruses Using Bioinformatics
Authors: Wadas Irena , Domingues Inês
Published: February 2025

The present paper addresses topics from various fields of biology. Its purpose is to enlarge the understanding of the usage of bioinformatics tools in the phylogenetic analysis of RNA viruses. The paper highlights the benefits of using information technology in virology, bringing the scientific community closer to unraveling the mysteries of RNA virus evolution and their adaptation to different niches and hosts and facilitating the understanding of their rapid mutation processes. Phylogenetic analysis of genetic sequences allows the exploration of the causes of these genetic changes in viruses and categorizes them into taxonomic groups. This paper is a systematic review of the most important scientific articles on the phylogenetic analysis of RNA viruses using bioinformatics. The studies included in the review were selected based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020) guidelines and discuss methods for analyzing genetic and protein sequences (including codon sequences) and describe phylogenetic analyses and the bioinformatics tools used (such as VConTACT, RAxML, etc.). This review emphasizes the importance of further development in the fields of bioinformatics and virology, particularly with respect to RNA viruses, in order to mitigate the risk of a future pandemic. It also aims to provide a detailed understanding of the mutation and evolution mechanisms of these entities, which will help in efforts to limit viral virulence, for example. This article did not receive any funding for its creation and has not been registered in any database.
Defining and Evaluating the Impact of Bleeding Severity on Time to Endoscopy and Mortality Risk: A Prospective Multicenter Cohort Study
Authors: Marmo Clelia , Bucci Cristina , Soncini Marco , Riccioni Maria Elena , Marmo Riccardo ,   on behalf of the GISED Study Group
Published: February 2025

Background: Upper gastrointestinal bleeding severity (BleSev) is commonly defined by evaluating different factors that are frequently interdependent on each other, expressing the same underlying cause. Aim: This study aimed to define the severity of a bleeding event and verify its impact on death risk and the time to endoscopy. Methods: We analyzed 12 factors (demographic, hemodynamic, biochemical, and clinical) that could be associated with BleSev. We identified the independent weight of each factor in predicting a composite endpoint (need for surgery, interventional radiology, and death) and the effect of the interactions between time to endoscopy and BleSev on death risk. Results: Clinical data of 2.525 patients were included. Of the 12 factors, 5 were retained in the final model as follows: altered mental status, systolic blood pressure ≤ 100 mmHg, blood urea nitrogen level ≥ 130 mg/dL, hematemesis, and hemoglobin level ≤ 8 g/dL (AUC performance curve, 0.79). We identified the following three classes of BleSev: low (0–1 points, 2.4%), intermediate (3–4 points, 8.6%), and high (≥5 points, 21.1%). When no factors were present, the death risk was 1%; when all factors were present, the risk was 45.5%. Notably, the death risk increased with BleSev but was generally independent of time to endoscopy. However, in high-risk cases, early endoscopy (within 6–12 h) was associated with a reduced mortality rate. Conclusions: This study defines a risk model for BleSev and highlights the need for targeted endoscopic timing strategies based on BleSev for optimizing survival rates. Patients in the highest risk category may benefit from more urgent endoscopic interventions.
Analyzing Declining Trends, Patient Demographics, and Complications in Total Elbow Arthroplasty: Nationwide Retrospective Data Analysis
Authors: Mahamid Assil , Jayyusi Fairoz , Laver Lior , Haj Yahya Mohammad , Wolff Gal , Yassin Ali , Behrbalk Eyal
Published: February 2025

Background: Total elbow arthroplasty (TEA) was initially introduced for end-stage rheumatoid arthritis but has since expanded to include osteoarthritis and complex distal humerus fractures, particularly in elderly patients. Over the past two decades, TEA utilization trends have fluctuated, with a recent decline attributed to advancements in disease-modifying antirheumatic drugs. Despite its benefits, TEA presents a high complication rate, necessitating further investigation into clinical outcomes, costs, and postoperative management. Methods: This retrospective cohort study analyzed TEA procedures from 2016 to 2019 using the National Inpatient Sample (NIS) database. Patients were identified via ICD-10 codes, with elective procedures included to ensure homogeneity. This study examined temporal trends, patient demographics, comorbidities, complication rates, length of stay (LOS), and hospitalization costs. Statistical analyses included chi-square tests, t-tests, and multivariate regression to assess associations between patient characteristics and outcomes. Results: A total of 4110 TEA procedures were analyzed, revealing a 16% decline in annual volume from 2016 to 2019 (p = 0.012). The cohort had a mean age of 65.99 years, with a predominance of female (75.3%) and White (72.6%) patients. The median LOS was two days, and median hospitalization costs were USD 78,473 (IQR: 56,935–115,671 USD). The most prevalent complications included mechanical loosening (12.5%), blood loss anemia (10.6%), cardiac complications (5.7%), and prosthetic-related pain (3.3%). Multivariate analysis identified hypertension, anemia, and respiratory disease as significant predictors of adverse outcomes. Conclusions: TEA utilization has declined, likely due to medical advancements in rheumatoid arthritis management. The procedure remains associated with substantial complication rates, particularly in trauma-related cases. Findings highlight the importance of patient optimization, surgical expertise, and postoperative monitoring to improve outcomes.
Assessment of Environmental Risk Factors for Gestational Diabetes Mellitus: A Ten-Year Systematic Review and Meta-Analysis
Authors: Tsokkou Sophia , Tzintros Stefanos-Timoleon , Konstantinidis Ioannis , Keramas Antonios , Georgaki Maria-Nefeli , Stamoula Eleni , Matsas Alkis
Published: February 2025

Background/Objectives: It is estimated that gestational diabetes mellitus (GDM) affects approximately 14% of pregnant women. This is due to the inability of the body to produce enough insulin for gestation. With greater appearance during the second and third trimesters, GDM has a multifactorial cause including hypertension, cardiovascular issues (CVD), family history both or either type two diabetes mellitus (T2DM) or GDM, obesity, advanced maternal age, and polycystic ovarian syndrome (PCOS). However, it has been suggested that except for genetic predisposition, environmental factors can increase the risk of GDM development to a great extent. The aim of this systematic review and meta-analysis is the examination of different environmental contributors that play a significant role in the development of GDM. Methods: The databases used were PubMed and ScienceDirect. The inclusion criteria were a 10-year duration (2014–2024), English language, research articles, and only humans included. Afterwards, tables were created to summarize the most important information from each article. Forest and funnel plots were created to assess the possibility of a greatly significant difference between each environmental contributor. Results: Initially, 9361 articles were found. After the automation tools were applied, 706 were left. The total number of articles used in the study after the screening process was 26. Through the systematic review analysis, the following risk factors were stated to play a contributing role with GDM: extreme temperatures (both high and low), organophosphorus flame retardants (OFRs), bisphenol A (BPA), selenium (Se), metallic elements, urinary antimony (Sb), trace elements, thiamine and riboflavin, and fine particulate matter PM2.5. Conclusions: Through this meta-analysis, it can be concluded that there is statistical significance for fine particulate matter PM2.5, especially in the first (p < 0.001) and second (p < 0.001) trimesters, proving the acknowledged connection between PM2.5 and GDM pathogenesis during pregnancy. Apart from that, fetal sex can play an important role in the development of GDM, as there is the greatest risk in males (p < 0.001), whereas no correlation between maternal smoking habit and bisphenol A with GDM was found. In conclusion, it can be stated that environmental factors can have a great impact on the development of GDM during the gestational period, but more studies must be carried out to reinforce our outcomes.
Occurrence of Jaw Osteonecrosis and Frequency of Prophylactic Tooth Extractions Prior to Head and Neck Radiotherapy: A Retrospective Study of 497 Irradiated Patients
Authors: Krause Inga , Hirsch Julius , Vorwerk Hilke , Stuck Boris A. , Neff Andreas
Published: February 2025

Background/Objectives: This retrospective study examined the relationship between prophylactic tooth extraction (PTE) and the occurrence of jaw osteoradionecrosis (JORN) in patients undergoing head and neck radiotherapy (HNR). The primary objective was to determine whether PTE resulted in a JORN rate comparable to that of patients who did not require or undergo PTE. Methods: A total of 497 patients were included. The primary predictor variable was PTE, and the primary outcome was JORN occurrence. Statistical analyses included univariate, bivariate, and multivariate regression, as well as Cox regression. The significance threshold was set at p ≤ 0.005. Results: JORN was more frequent in the PTE group than in patients who did not require or undergo PTE (17.1% vs. 13.0%; hazard ratio [HR] 1.71, 95% CI: 1.08–2.71, p = 0.021). However, a significant association could not be confirmed using multiple logistic regression (odds ratio [OR] 1.36, 95% CI: 0.82–2.26, p = 0.236). Suggestive associations were observed for HNR dose (HR 1.03 per Gy, p = 0.007) and tumor location (pharyngeal HR 0.52, p = 0.03; laryngeal HR 0.51, p = 0.02). Conclusions: Patients with PTE showed a higher JORN rate but the findings were only marginally significant, and no causal relationship was established. The differing results between Cox and logistic regression suggest a time-dependent effect of PTE, with an increased early risk for JORN. Further studies are needed to determine whether greater emphasis should be placed on tooth-preserving measures, limiting extractions before HNR to strictly non-preservable teeth.
Correction: Ko et al. Timing of Mouse Molar Formation Is Independent of Jaw Length Including Retromolar Space. J. Dev. Biol. 2021, 9, 8
Authors: Ko Daisy (Jihyung) , Kelly Tess , Thompson Lacey , Uppal Jasmene K. , Rostampour Nasim , Webb Mark Adam , Zhu Ning , Belev George , Mondal Prosanta , Cooper David M. L. , Boughner Julia C.
Published: February 2025

There was an error in the original publication [...]
Investigating the Predictive Performance of Process Data and Result Data in Complex Problem Solving Using the Conditional Gradient Boosting Algorithm
Authors: Aydin Fatma Nur , Atalay Kabasakal Kubra , Dilek Ismail
Published: February 2025

This study aims to examine the predictive performance of process data and result data in complex problem-solving skills using the conditional gradient boosting algorithm. For this purpose, data from 915 participants of the 2012 cycle of the Programme for International Student Assessment (PISA) were utilized. Process data were obtained from the log file of the first question in the climate control unit task included in the problem-solving assessment of PISA 2012. Various cognitive and affective attributes from the same assessment were used as the result data. According to the results, (1) process data demonstrated a moderate, result data demonstrated a moderate-to-good, and process + result data demonstrated a good prediction performance. (2) The most effective variables were the VOTAT (vary-one-thing-at-a-time) strategy score and total time in process data; the mathematical literacy and reading literacy scores in result data; and the mathematical literacy and VOTAT strategy score in process + result data. The dominance of the mathematical literacy has been noteworthy.
The Nonsense of Bitcoin in Portfolio Analysis
Authors: Shalit Haim
Published: February 2025

The paper demonstrates the nonsense of using Bitcoin in financial investments. By using mean-variance financial analysis, stochastic dominance, CVaR, and the Shapley value theory as analytical statistical models, I show how Bitcoin performs poorly by comparing it against other traded assets. The conclusion is reached by analyzing daily freely available market data for the period 2018–2023.
Structure of the Inhibited Smooth Muscle Myosin and Its Implications on the Regulation of Insect Striated Muscle Myosin
Authors: Sun Shaopeng , Lu Yi-Ning , Li Xiang-dong
Published: February 2025

Class II myosin (myosin-2) is an actin-based motor protein found in nearly all eukaryotes. One critical question is how the motor function of myosin-2 is regulated. Vertebrate myosin-2 comprises non-muscle myosin, smooth muscle myosin and striated muscle myosin. Recent studies have shown that smooth muscle myosin, in its inhibited state, adopts a folded conformation in which the two heads interact with each other asymmetrically, and the tail is folded into three segments that wrap around the two heads. It has been proposed that the asymmetric head-to-head interaction is a conserved, fundamental structure essential for the regulation of all types of myosin-2. Nearly all insects have only a single striated muscle myosin heavy chain (MHC) gene, which produces all MHC isoforms through alternative splicing of mutually exclusive exons. Most of the alternative exon-encoded regions in insect MHC are located in the motor domain and are critical for generating isoform-specific contraction velocity and force production. However, it remains unclear whether these alternative exon-encoded regions participate in the regulation of insect striated muscle myosin. Here, we review the recently resolved structure of the inhibited state of smooth muscle myosin and discuss its implications on the regulation of insect striated muscle myosin. We propose that the alternative exon-encoded regions in insect MHC not only affect motor properties but also contribute to stabilizing the folded conformation and play a crucial role in regulating insect striated muscle myosin.
Technical and Performance Characteristics Between Different Anti-Mullerian Hormone (AMH) Assay Methods and Antral Follicle Count (AFC) in Malaysian Women with Infertility: A University-Based Centre Cohort
Authors: Wen Chong Jie , Ahmad Mohd Faizal , Abu Muhammad Azrai , Johari Nalisa Shamyra , Badaruddin Izzatul Aliaa , Azhar Shah Shamsul , Abdul Karim Abdul Kadir
Published: February 2025

Backgrounds: Evaluating the AMH level and AFC are crucial in infertility practice. Thus, accurate measurement is paramount. Various methods are available and selecting the right process is essential to ensure good reproducibility and precise results. Thus, our study aims to determine the analytical performance of AMH Plus and AFIAS-AMH and the correlation between the two AMH assays and AFC values. Methods: A prospective study was conducted at the Advanced Reproductive Center, Hospital Canselor Tuanku Mukhriz (HCTM), Faculty of Medicine, National University of Malaysia, to compare the correlation between the AMH assay methods and AFCs. It included 100 patients from Feb 2024 until June 2024. Results: A total of 100 women with infertility, with a mean age of 35.87 ± 3.92, were included in our study. Our results revealed a strong positive correlation between the two AMH assays, with the comparable performance of AMH Plus and AFIAS-AMH. We also found that the serum AMH evaluation was comparable with the routine AFC assessment. Conclusions: Our findings indicated that serum AMH levels determine the association between AMH levels and follicle counts and the correlation between AMH concentrations and assays. Our study demonstrated the competent repeatability, acceptable linearity, and laboratory precision of the AFIAS-AMH test and comparative assessment of the AFC and serum AMH evaluation.
Influence of Steel Fiber Content on the Long-Term Stability of Slag-Containing UHPC Under Different Environments
Authors: Liao Gaoyu , Xu Yikui , Wang Dianchao , Wu Linmei
Published: February 2025

Ultra-high-performance concrete (UHPC) is an advanced material known for its high strength and durability. This study investigates the long-term stability of slag-containing UHPC exposed to outdoor, tap water, and seawater environments over 720 days, focusing on the impact of steel fiber content. The results show that UHPC in tap water exhibits higher compressive strength compared to seawater and outdoor environments. Without fibers, compressive strengths at 1 day, 28 days, and 720 days are 99.7 MPa, 104.7 MPa, and 148.0 MPa, respectively. With 3% steel fiber, these values increase to 132.9 MPa, 143.6 MPa, and 166.6 MPa, representing increases of 33.3%, 37.2%, and 12.6%. Steel fibers also reduce the expansion rate of UHPC, with the expansion decreasing from 525 × 10−6 (no fibers) to 345 × 10−6 (3% fibers), a reduction of 34.3%. Additionally, differential thermogravimetry (DTG) analysis further confirms the formation of C-S-H, ettringite, calcium hydroxide, and calcite under different environments.
Mechanical and Microstructural Performance of UHPC with Recycled Aggregates Modified by Basalt Fiber and Nanoalumina at High Temperatures
Authors: Jiang Hong , Luo Liang , Hou Yuan , Yang Yifei
Published: February 2025

This study investigates the mechanical properties and microstructure of basalt fiber (BF) and nanoalumina (NA)-modified ultra-high-performance concrete with recycled aggregates (UHPC-RA) under high-temperature conditions. The effects of different replacement rates of recycled aggregates (RAs), BF content, and NA content on the compressive strength, splitting tensile strength, and elastic modulus were evaluated at ambient temperatures and after exposure to 200 °C, 400 °C, 600 °C, and 800 °C. The results show that mechanical properties decrease with temperature rise, but specimens containing BF exhibited improved crack resistance and better high-temperature integrity. The incorporation of NA enhanced the thermal stability and heat resistance of the concrete. Digital image correlation (DIC) was used to monitor real-time surface deformation, and scanning electron microscopy (SEM) analysis revealed improved microstructure with reduced porosity and cracks. This study demonstrates that the combination of BF and NA significantly enhances the high-temperature performance of UHPC-RA, which holds promising potential for applications in environments subjected to elevated temperatures.
Effect of Curing Temperature on Volume Changes of Alkali-Activated Slag Pastes
Authors: Lacante Maïté , Delsaute Brice , Staquet Stéphanie
Published: February 2025

This study investigates the influence of curing temperature (explored at 10 °C, 20 °C, and 30 °C) on the volume changes of alkali-activated slag (AAS) pastes with the aim of expanding existing knowledge on alkali-activated materials (AAMs). The focus was on autogenous and thermal strains, internal relative humidity (IRH), heat flow and cumulative heat, setting times, and workability. The results indicate that increasing the curing temperature to 30 °C reduces autogenous shrinkage, likely due to changes in the elastic modulus and viscoelastic properties, while promoting swelling, especially for higher molarities. The coefficient of thermal expansion (CTE), related to thermal strains, is higher when the curing temperature is increased, but its development is delayed. The IRH is influenced more by the activating solution’s molarity than by curing temperature, although temperature does affect the initial IRH. The study also revealed that higher curing temperatures accelerate chemical reactions and reduce setting times. The initial workability was significantly affected by the solution-to-binder ratio, while higher temperatures decreased workability, especially at higher molarities. These findings contribute to the understanding of how curing temperature influences the durability of AAS pastes, offering insights into optimized construction practices under varying environmental conditions.
Modeling of Electrical Heating and Cooling for Carbon Textile Reinforced Concrete
Authors: Dahlhoff Annette , Raupach Michael
Published: February 2025

Carbon-textile-reinforced concrete (CTRC) is increasingly being used in the construction industry as a high-performance composite material combining non-metallic textile reinforcement with concrete. Known for its exceptional characteristics such as tensile strength, density, and durability, CTRC also exhibits electrical conductivity, enabling efficient electrical heat generation within building components. This study develops and validates a thermal model to predict the temperature evolution of electrically heated CTRC, incorporating Newton’s law of cooling and Joule’s heating principle. The proposed model segments the temperature development into three distinct phases: heating, constant, and cooling. The temperature calculation accounts for these phases, their boundary conditions, and material-specific parameters, which were determined through laboratory experiments. For the investigated CTRC material combinations, the model accurately predicts temperature profiles, demonstrating strong agreement between experimental and calculated results. Moreover, significant variations in electrical power requirements were observed among the tested materials. The investigated impregnation materials of the carbon textile reinforcement (CTR) significantly influence contact quality and resulting temperature behavior. This research bridges material science and thermal performance, expanding the potential for CTRC use in electrically heated construction solutions.
Preparation of Phosphate Glass by the Conventional and Microwave Melt-Quenching Methods and Research on Its Performance
Authors: Li Baiyi , Zhou Guangdong
Published: February 2025

In this study, various phosphate glasses (PGs) within the CaO-Na2O-P2O5 and SiO2-CaO-Na2O-P2O5 systems were synthesized using both conventional and microwave melt-quenching techniques. The physical, thermal, and structural properties of these phosphate glasses, as well as their dissolution behaviors under varying temperatures and solvent conditions, were thoroughly examined. Additionally, the influence of scale inhibitor dosage, temperature, and pH on the rate of scale inhibition was assessed using a static scale inhibition test. The morphology and crystal types of the precipitates were characterized using Scanning Electron Microscopy (SEM) and X-ray Diffraction (XRD) analysis. The findings indicate that the PG structure predominantly consists of Q2 structural units, with a minor presence of Q1 units. The dissolution rate of PG escalates with an increase in temperature and a decrease in pH. Conversely, the scale inhibition efficiency diminishes with rising temperature and pH. Optimal scale inhibition efficiency, reaching up to 95.4%, was observed at a pH of 7 and a temperature of 50 °C. Under the influence of the PG scale inhibitor, the primary crystal form of CaCO3 was altered from calcite to vaterite.
Valorization of Banana Peel Waste into Advanced Adsorbent Beads for the Removal of Emerging Pollutants from Wastewater
Authors: Boyle Olivia , Xiao Bo , Mangwandi Chirangano
Published: February 2025

This study addresses environmental concerns by utilizing banana peel waste to develop innovative adsorbent materials for wastewater treatment, aligning with circular economy principles. Spherical beads were synthesized from sodium alginate mixed with various banana peel-based materials, including pure powder (PBP), activated carbon (AC), and magnetic activated carbon (MAC). These beads were evaluated for their efficiency in removing tetracycline (TC) and hexavalent chromium (Cr(VI)) as model pollutants representing antibiotics and heavy metals, respectively. Characterization of the beads revealed functional groups and thermal stability conducive to effective adsorption. Adsorption trials demonstrated that MAC beads achieved the highest removal efficiencies, up to 92% for TC and 79% for Cr(VI). The adsorption process followed pseudo-second-order kinetics and Langmuir isotherms. Remarkably, the beads retained a significant adsorption capacity across reuse cycles, indicating their regenerative potential. Comparisons with other adsorbents highlight the competitive performance of these banana peel-based materials. The results emphasize the potential of banana peel-derived adsorbents as cost-effective, sustainable solutions for mitigating emerging pollutants in water systems, promoting waste valorization and environmental protection. The research demonstrates a novel approach to sequential adsorption without intermediate regeneration, showing that the beads can effectively remove both tetracycline and chromium (VI) in successive cycles. This finding is particularly significant because it reveals that the presence of previously adsorbed chromium actually enhanced the beads’ capacity for tetracycline removal in the second cycle, suggesting a synergistic effect that had not been previously reported in the literature. These innovations contribute meaningfully to both waste valorization and water treatment technologies, offering new insights into the development of multi-functional adsorbents from agricultural waste materials.
Roles of Water Molecules in the Structures and Magnetic Properties of Coordination Polymers with a Dicarboxylate Ligand
Authors: Zong Dehui , Gao En-Qing , Zhang Dawei
Published: February 2025

Three new coordination polymers, {[M(nbpdc)(DMF)(H2O)2]·H2O}∞ (M = Co and Ni) and [Zn(nbpdc)(DMF)(H2O)]∞, were synthesized from 2-nitrobiphenyl-4,4′-dicarboxylate (nbpdc2−). The isomorphous Co(II) and Ni(II) compounds exhibited a two-dimensional coordination network in which the chains with single-water bridges and the chains with single-nbpdc2− bridges intersected each other by sharing the metal ions. The coordination networks were connected with uncoordinated water molecules through hydrogen bonds. The rarely identified single-water-bridged coordination chain was reinforced by water-based intrachain hydrogen bonds. The single-water bridges mediated modest antiferromagnetic superexchange in both Co(II) and Ni(II) compounds and afforded a spin-canting structure for the Co(II) compound at low temperatures. Water molecules played a distinct structural role in the Zn(II) compound, which was a one-dimensional coordination polymer with single-nbpdc2− bridges. Instead of bridging metal ions, each water molecule was coordinated to one metal ion and hydrogen-bonded to the coordination spheres of other two metal ions, resulting to an infinite ladderlike hydrogen-bonding motif. The ladders interlinked the nbpdc-bridged chains into a three-dimensional supramolecular architecture featuring the 5-conneted {44.64} net.