bluuebunny/crossref_metadata_2025 huggingface dataset:
Maize Zmcyp710a8 Mutant as a Tool to Decipher the Function of Stigmasterol in Plant Metabolism
Authors: Aboobucker Siddique I. , Showman Lucas J. , Lübberstedt Thomas , Suza Walter P.
Published: November 2021

Sterols are integral components of membrane lipid bilayers in eukaryotic organisms and serve as precursors to steroid hormones in vertebrates and brassinosteroids (BR) in plants. In vertebrates, cholesterol is the terminal sterol serving both indirect and direct roles in cell signaling. Plants synthesize a mixture of sterols including cholesterol, sitosterol, campesterol, and stigmasterol but the signaling role for the free forms of individual plant sterols is unclear. Since stigmasterol is the terminal sterol in the sitosterol branch and produced from a single enzymatic step, modifying stigmasterol concentration may shed light on its role in plant metabolism. Although Arabidopsis has been the model of choice to study sterol function, the functional redundancy of AtCYP710A genes and the presence of brassicasterol may hinder our ability to test the biological function of stigmasterol. We report here the identification and characterization of ZmCYP710A8, the sole maize C-22 sterol desaturase involved in stigmasterol biosynthesis and the identification of a stigmasterol-free Zmcyp710a8 mutant. ZmCYP710A8 mRNA expression pattern correlated with transcripts for several sterol biosynthesis genes and loss of stigmasterol impacted sterol composition. Exogenous stigmasterol also had a stimulatory effect on mRNA for ZmHMGR and ZmSMT2. This demonstrates the potential of Zmcyp710a8 in understanding the role of stigmasterol in modulating sterol biosynthesis and global cellular metabolism. Several amino acids accumulate in the Zmcyp710a8 mutant, offering opportunity for genetic enhancement of nutritional quality of maize. Other cellular metabolites in roots and shoots of maize and Arabidopsis were also impacted by genetic modification of stigmasterol content. Yet lack of obvious developmental defects in Zmcyp710a8 suggest that stigmasterol might not be essential for plant growth under normal conditions. Nonetheless, the Zmcyp710a8 mutant reported here is of great utility to advance our understanding of the additional roles of stigmasterol in plant metabolism. A number of biological and agronomic questions can be interrogated using this tool such as gene expression studies, spatio-temporal localization of sterols, cellular metabolism, pathway regulation, physiological studies, and crop improvement.
Personality Factors in Colorectal Cancer: A Systematic Review
Authors: Galli Federica , Scotto Ludovica , Ravenda Simona , Zampino Maria Giulia , Pravettoni Gabriella , Mazzocco Ketti
Published: November 2021

Background: The role of personality in cancer incidence and development has been studied for a long time. As colorectal cancer (CRC) is one of the most prevalent cancer types and linked with lifestyle habits, it is important to better understand its psychological correlates, in order to design a more specific prevention and intervention plan. The aim of this systematic review is to analyze all the studies investigating the role of personality in CRC incidence.

Methods: All studies on CRC and personality up to November 2020 were scrutinized according to the Cochrane Collaboration and the PRISMA statements. Selected studies were additionally evaluated for the Risk of Bias according to the Newcastle-Ottawa Scale (NOS).

Results: Eight studies met the inclusion criteria and were eventually included in this review. Two main constructs have been identified as potential contributors of CRC incidence: emotional regulation (anger) and relational style (egoism).

Conclusion: Strong conclusions regarding the influence of personality traits on the incidence of CRC are not possible, because of the small number and the heterogeneity of the selected studies. Further research is needed to understand the complexity of personality and its role in the incidence of CRC and the interaction with other valuable risk factors.
The Transition From Maternity Blues to Full-Blown Perinatal Depression: Results From a Longitudinal Study
Authors: Luciano Mario , Sampogna Gaia , Del Vecchio Valeria , Giallonardo Vincenzo , Perris Francesco , Carfagno Marco , Raia Maria Luce , Di Vincenzo Matteo , La Verde Marco , Torella Marco , Fiorillo Andrea
Published: November 2021

Background: The aims of the present study are to: (1) assess the frequency of maternity blues (MB); (2) identify the clinical and social characteristics more frequently associated with the onset of depressive symptoms after delivery; and (3) verify the hypothesis that the presence of maternity blues is a risk factor for the onset of a full-blown depressive episode in the 12 months after delivery.

Methods: This is a longitudinal observational study. All pregnant women who gave birth at the inpatient unit of Gynecology and Obstetrics of the University of Campania “Luigi Vanvitelli” from December 2019 to February 2021 have been invited to participate in the study. Upon acceptance, women were asked to complete the Italian version of the Edinburgh Postnatal Depression Scale along with an ad-hoc questionnaire on the women's sociodemographic, gynecological and peripartum characteristics as well as their psychiatric history. Women have been reassessed after one, 3, 6 and 12 months.

Results: A total of 359 women were recruited within 3 days from delivery, with a mean EPDS total score of 5.51 (±4.20). Eighty-three women (23.1%) reported the presence of maternity blues. Mean EPDS total scores were 12.8 (±0.2) in the MB group vs. 4.26 (±0.2) in the group without MB (p <0.0001). MB predictors were the presence of an anxiety disorder with an onset 6 months prior to pregnancy, of preeclampsia, of increased fetal health rate, of conflicts with relatives other than partner and having a partner with an anxiety disorder. At multivariate analyses the presence of MB increased 7-time the risk to have a higher EPDS score at follow-up assessments (OR: 7.79; CI: 6.88–8.70, p <0.000). This risk is almost four times higher 1 months after the delivery (OR: 4.66; CI: 2.54–6.75, p < 0.000), almost three times higher after 3 months (OR: 2.98; CI: 0.50–5.46, p < 0.01) and almost six times higher after 12 months (OR: 5.88; CI: 3.20–8.54, p < 0.000).

Conclusions: Although MB was a self-limiting condition in the majority of cases, depressive symptoms arose quite often immediately after the childbirth. Professionals should be trained to monitor symptoms of MB and its transition toward a depressive episode.
Investigating Changes in Reward-Related Neural Correlates After PEERS Intervention in Adolescents With ASD: Preliminary Evidence of a “Precision Medicine” Approach
Authors: Baker Elizabeth , Veytsman Elina , Choy Tricia , Blacher Jan , Stavropoulos Katherine K. M.
Published: November 2021

Background: The Social Motivation Hypothesis proposes that individuals with autism spectrum disorder (ASD) experience social interactions as less rewarding than their neurotypical (TD) peers, which may lead to reduced social initiation. Existing studies of the brain's reward system in individuals with ASD report varied findings for anticipation of and response to social rewards. Given discrepant findings, the anticipation of and response to social rewards should be further evaluated, particularly in the context of intervention outcome. We hypothesized that individual characteristics may help predict neural changes from pre- to post-intervention.

Methods: Thirteen adolescents with ASD received the Program for the Education and Enrichment of Relational Skills (PEERS) intervention for 16 weeks; reward-related EEG was collected before and after intervention. Fourteen TD adolescents were tested at two timepoints but did not receive intervention. Event-related potentials were calculated to measure anticipation of (stimulus-preceding negativity; SPN) and response to (reward-related positivity; RewP) social and non-social rewards. Additionally, measures of social responsiveness, social skills, and intervention-engagement were collected. Group differences were analyzed as well as individual differences using prediction models.

Result: Parent-reported social responsiveness and social skills improved in adolescents with ASD after participation in PEERS. ASD adolescents displayed marginally decreased anticipation of social rewards at post-intervention compared to pre-intervention. Regression models demonstrated that older adolescents and those with lower parent-reported social motivation prior to participation in PEERS displayed marginally increased social reward anticipation (more robust SPN) from pre- to post-intervention. Participants who displayed more parent-reported social motivation before intervention and were more actively engaged in the PEERS intervention evidenced increased social reward processing (more robust RewP) from pre- to post-intervention.

Conclusion: Findings suggest that there may be differences in saliency between wanting/anticipating social rewards vs. liking/responding to social rewards in individuals with ASD. Our findings support the hypothesis that identification of individual differences may predict which adolescents are poised to benefit the most from particular interventions. As such, reported findings set the stage for the advancement of “precision medicine.” This investigation is a critical step forward in our ability to understand and predict individual response to interventions in individuals with ASD.
Investigation of the Cognitive Attitudes and Behaviors of Medical Post-graduates in Clinical Practice During the COVID-19 Pandemic in China
Authors: Feng Lei , Xie Zefeng , Shen Junhui
Published: November 2021

The cognitive attitudes and behaviors of medical post-graduates may be influenced by the coronavirus disease 2019 (COVID-19) pandemic. A cross-sectional study was used from a questionnaire survey in hospitals affiliated with the Zhejiang University School of Medicine. Questionnaire was distributed online including demographic information, cognitive attitudes, and personal protective behaviors. Moreover, personal protective behaviors such as wearing protective equipment were compared between different academic major and gender, respectively. A total of 176 valid questionnaires were obtained. Of the medical post-graduates in this study, (1) 89.67% believed that the COVID-19 pandemic had an impact on their clinical internships, and 40.34% expressed concerns about their infection on inadequate personal protection; (2) 91.48% took personal protection in hospital and 86.36% enhanced personal hygiene; (3) There were no statistically differences in the personal protection by academic major and gender (p > 0.05). This study suggests that the COVID-19 pandemic had an impact on the medical post-graduates' clinical practice, and affected their cognitive attitudes and behaviors. As such, universities and hospitals should increase pandemic prevention training and investment, provide more psychological counseling to their medical post-graduates to reduce their psychological burden, and take measures to reduce the influence of the COVID-19 pandemic on their medical post-graduates' clinical practice.
Diminished Pneumococcal-Specific CD4+ T-Cell Response is Associated With Increased Regulatory T Cells at Older Age
Authors: He Samantha W. J. , van de Garde Martijn D. B. , Pieren Daan K. J. , Poelen Martien C. M. , Voß Franziska , Abdullah Mohammed R. , Hammerschmidt Sven , van Els Cécile A. C. M.
Published: November 2021

Respiratory infection caused by Streptococcus pneumoniae is a leading cause of morbidity and mortality in older adults. Acquired CD4+ T cell mechanism are essential for the protection against colonization and subsequent development of infections by S. pneumoniae. In this study, we hypothesized that age-related changes within the CD4+ T-cell population compromise CD4+ T-cell specific responses to S. pneumoniae, thereby contributing to increased susceptibility at older age. To this end, we interrogated the CD4+ T-cell response against the immunogenic pneumococcal protein AliB, part of the unique oligopeptide ABC transporter system responsible for the uptake of nutrients for the bacterium and crucial for the development of pneumococcal meningitis, in healthy young and older adults. Specifically, proliferation of CD4+ T cells as well as concomitant cytokine profiles and phenotypic markers implied in immunosenescence were studied. Older adults showed decreased AliB-induced CD4+ T-cell proliferation that is associated with an increased frequency of regulatory T cells and lower levels of active CD25+CD127+CTLA-4−TIGIT-CD4+T cells. Additionally, levels of pro-inflammatory cytokines IFNy and IL-17F were decreased at older age. Our findings indicate that key features of a pneumococcal-specific CD4+ T-cell immune response are altered at older age, which may contribute to enhanced susceptibility for pneumococcal infections.
Toward Automatically Labeling Situations in Soccer
Authors: Fassmeyer Dennis , Anzer Gabriel , Bauer Pascal , Brefeld Ulf
Published: November 2021

We study the automatic annotation of situations in soccer games. At first sight, this translates nicely into a standard supervised learning problem. However, in a fully supervised setting, predictive accuracies are supposed to correlate positively with the amount of labeled situations: more labeled training data simply promise better performance. Unfortunately, non-trivially annotated situations in soccer games are scarce, expensive and almost always require human experts; a fully supervised approach appears infeasible. Hence, we split the problem into two parts and learn (i) a meaningful feature representation using variational autoencoders on unlabeled data at large scales and (ii) a large-margin classifier acting in this feature space but utilize only a few (manually) annotated examples of the situation of interest. We propose four different architectures of the variational autoencoder and empirically study the detection of corner kicks, crosses and counterattacks. We observe high predictive accuracies above 90% AUC irrespectively of the task.
Plant Disease Diagnostic Capabilities in Venezuela: Implications for Food Security
Authors: Marys Edgloris , Rosales Ligia Carolina
Published: November 2021

Venezuela is currently experiencing the most severe humanitarian crisis in the Americas of this century. Little food is being produced locally, despite the population's right to food. Plant disease outbreaks are causing substantial declines in major staple food and cash crops, and this impacts on rural livelihoods, and poses a significant and growing threat to the already complex food insecurity crisis in the country. Nonetheless, phytosanitary services and hence the control of plant pests and diseases have been substantially weakened over the recent years as a consequence of the collapse of the economy and the substantial deterioration of government services. Therefore, most of the pathogens associated with symptoms-causing diseases remain unidentified or uncharacterized, and no surveillance or crop protection strategies have been implemented. In this review, we address the country's issues and challenges in diagnosing, monitoring and managing plant diseases to restore national food security.
Suprapubic Transvesical Repair of Vesicovaginal Fistula Using a Homemade Laparoscopic Single-Port Device: Experience of 42 Patients
Authors: Fan Xinxiang , Ma Xiaoming , Lai Yiming , Li Zean , Huang Jian , Huang Hai
Published: November 2021

Aim: Vesicovaginal fistula (VVF) is the most common urogenital acquired fistula, and has remained a scourge and of public health importance. VVF can be repaired by transvaginal approach, transabdominal approach or transvesical approach, but the optimal management is still debated.

Methods: To demonstrate a suprapubic transvesical approach to repair VVFs using a homemade laparoscopic single-port device. A retrospective review of the medical records of 42 consecutive patients who underwent fistula repair for VVF at our center from January 2012 to March 2018 was performed. VVFs were repaired by a suprapubic transvesical approach using a homemade laparoscopic single-port device. Clinical data, perioperative data and outcomes were collected. The primary outcome was VVF successful closure rate, and secondary outcome was perioperative complications.

Results: The mean age of the patients was 44.6 (27–58) yr. The mean follow-up time was 65.6 (32–118) mo. The VVFs were successfully closed in 37 (88.1%) patients after the first surgery, and failure was observed in five patients. Initial failures of all the five patients were cured after a second repair. No major complication occurred as defined by Clavien-Dindo class 2 or greater.

Conclusions: Suprapubic transvesical approach to repair VVFs using a homemade laparoscopic single-port device is a simple, effective, and feasible approach offering ideal results without major complications.
A Comparison of the Ability of Some Commercially Produced Biological Control Agents to Protect Strawberry Plants against the Plant Pathogen Phytophthora cactorum
Authors: Pánek Matěj , Hanáček Aleš , Wenzlová Jana , Maňasová Marie , Zouhar Miloslav
Published: November 2021

A comparison of the ability of commercially produced biological control agents—Contans, Gliorex, Hirundo, Polyversum, Prometheus, Clonoplus, Integral Pro and Xilon GR, completed with an isolate of Clonostachys rosea and of Pseudomonas sp.—to protect strawberry plants against Phytophthora cactorum was performed. The experiment was performed on strawberry cultivars Sonata, Karmen, and Wendy—cultivated in a cultivating room and greenhouse. The health of plants was affected negatively by the pathogen in all variants of biological agents used, but differences were seen in the rates of this decrease. The results revealed the ability of some tested agents to improve the growth of plants in the absence of the pathogen; the preparation Polyversum (Pythium oligandrum) was the most beneficial, in both the presence and absence of the pathogen. Contrarily, some agents alone decreased the health of plants; Integral Pro (Bacillus subtillis) and a strain of Pseudomonas sp. caused a deterioration in the health of the plants, even in the absence of a pathogen. The results of our analysis demonstrate the varied usefulness of all agents under unified environmental conditions; their effect seems to be dependent on the conditions and on the combination of the genotypes of all three participants in the interaction: plant–pathogen–antagonist.
Agricultural Holdings’ Impact on the Rural Development. Case Study: Romania
Authors: Ionescu Romeo Victor , Zlati Monica Laura , Antohi Valentin Marian , Florea Andrei Mirel , Bercu Florentin , Buhociu Florin Marian
Published: November 2021

Associative forms represent a part of the agricultural producers’ manifestation, with long-term impact on rural development. Considering the current dimension and the cultural-organizational baggage, the authors aim to carry out an impact study on the contribution of agricultural holding companies to rural development. This objective is to be achieved through prospective analysis methods based on a structured questionnaire, which allows for statistical tests of the variables’ frequency and representativeness and econometric modelling of rural development efficiency in relation to independent variables related to the organizational custom and economic outputs of organizations. The results of the study aim at identifying the vulnerabilities that hinder the maximization of the function of the agricultural holding companies (the economic development) and treating these vulnerabilities through some concrete measures according to the modelling results. The study is useful for rural policy makers and trainers in any country in the world.
Optimal Standardized Ileal Digestible Total Sulfur Amino Acids to Lysine REQUIREMENTS Are Increased in Nursery Pigs Raised under Antibiotic-Free Feeding Regime
Authors: Ren Ping , Almeida Ferdinando , Orlando Uislei , Gonçalves Marcio , Hancock Deana , Vazquez-Añón Mercedes
Published: November 2021

This study aimed to investigate the effect of increasing the standardized ileal digestible (SID) total sulfur amino acid to lysine (TSAA:Lys) on the growth performance of nursery pigs raised with or without antibiotics (AGP) and to determine the optimal SID TSAA:Lys in nursery pigs raised without AGP. In Exp. 1, 924 nursery pigs (7.9 ± 1.3 kg), blocked by initial BW and sex, were randomly allotted to one of six treatments, with seven pens per treatment and twenty-two pigs per pen. The treatments were arranged in a 2 × 3 factorial design, with two AGP levels (0 or 50 mg/kg Carbodox) and three levels of SID TSAA:Lys (51.0, 58.5 or 66.0%). In Exp. 2, 990 weaned piglets (5.1 ± 0.9 kg), blocked by initial BW and sex, were randomly allotted to one of five dietary treatments (SID TSAA:Lys at 51, 58, 65, 72 or 79%) in the absence of AGP, with nine pens per treatment and twenty-two pigs per pen. Competing heteroskedastic models including broken-line linear (BLL), broken-line quadratic (BLQ), and quadratic polynomial (QP) were fitted for the growth performance data to estimate the optimal TSAA:Lys. In Exp. 1, AGP supplementation increased (p < 0.05) ADG and ADFI during the 21 d period. Increasing SID TSAA:Lys in the diets with AGP did not affect growth performance; however, increasing SID TSAA:Lys in the diets without AGP resulted in a linear increase (p < 0.05) in ADG and G:F. In Exp. 2, the best-fitting models for ADG and G:F from d 0 to 21 post-weaning were BLL, which yielded the optimal SID TSAA:Lys of 62% and 72%, respectively. The best-fitting models for ADG and G:F from d 21 to 42 post-weaning were BLL, which yielded the optimal SID TSAA:Lys of 59% and 58%, respectively. In conclusion, SID TSAA to Lys requirements under an antibiotic-free feeding regime during the first 21 d post-weaning were 62% and 72% in terms of ADG and G:F, respectively, whereas an SID TSAA:Lys of approximately 58% was required to maximize ADG and G:F for the late nursery phase.
Green and Chemical Silver Nanoparticles and Pomegranate Formulations to Heal Infected Wounds in Diabetic Rats
Authors: Scappaticci Renan Aparecido Fernandes , Berretta Andresa Aparecida , Torres Elina Cassia , Buszinski Andrei Felipe Moreira , Fernandes Gabriela Lopes , dos Reis Thaila Fernanda , de Souza-Neto Francisco Nunes , Gorup Luiz Fernando , de Camargo Emerson Rodrigues , Barbosa Debora Barros
Published: November 2021

Infected cutaneous ulcers from diabetic rats with Candida albicans and Streptococcus aureus were treated with spray formulations containing green silver nanoparticles (GS), chemical silver nanoparticles (CS), or pomegranate peel extract (PS). After wound development and infection, the treatments were performed twice per day for 14 days. The wound healing was analyzed on days 2, 7, and 14 through the determination of CFUs, inflammatory infiltrate, angiogenesis, fibroplasia, myeloperoxidase, and collagen determination. Expressive improvement in wound healing was noted using both silver nanoparticles for 7 days. All the treatments were superior to controls and promoted significant S. aureus reduction after 14 days. CS presented better anti-inflammatory results, and GS and CS the highest number of fibroblasts. Despite the techniques’ limitations, GS and CS demonstrated considerable potential for managing infected wounds, especially considering no early strategies prior to the drugs, such as the debridement of these wounds, were included.
Fabrication of Sinusoidal Microstructures on Curved Copper Surface by Ultra-Precision Diamond Cutting with a Rotary B-Axis and Fast Tool Servo System
Authors: Zhao Xuesen , Du Haiping , Song Wenda , Zhang Qiang , Hu Zhenjiang , Zhang Junjie , Sun Tao
Published: November 2021

While curved surface microstructures have wide applications in optical components and devices, how to achieve high machining accuracy of the microstructures is crucial for their applications. In the present work, we fabricate sinusoidal modulation microstructures on a curved copper surface by ultra-precision diamond cutting, with the combination of a rotary B-axis and a fast tool servo system. Specifically, tool path planning, together with the consideration of a curved, sinusoidal surface meshing and tool tip arc segmentation compensation, is carried out. Preliminary cutting experiments are firstly carried out on a homemade four-axis ultra-precision lathe, which demonstrates the advantages of additionally applying the rotary B-axis in suppressing burr formations and over-cutting phenomenon over the sole utilization of the fast tool servo system. Subsequent experiments are carried out to evaluate the effects of feed rate and the number of sampling points on the machining accuracy of the microstructures under the combination of a rotary B-axis and a fast tool servo system. With the optimized machining parameters, sinusoidal modulation microstructures, which have a wavelength of 700.6 μm, a peak-to-valley of 18.7 μm, a surface roughness of 18.9 nm and a deviation of profile tolerance of 4.326 μm, are successfully fabricated on a curved copper surface with a face radius of 10 mm and a curvature radius of 500 mm.
Identification of Scopoletin and Chlorogenic Acid as Potential Active Components in Sunflower Calathide Enzymatically Hydrolyzed Extract towards Hyperuricemia
Authors: Dai Huining , Lv Shuai , Fu Xueqi , Li Wannan
Published: November 2021

It is known that sunflower (Helianthus annuus L.) calathide enzymatically hydrolyzed extract (SCHE) contributes to the regulation of serum uric acid (UA); however, evidence regarding its bioactive components and mechanism are lacking. We identified two water-soluble components (scopoletin and chlorogenic acid) that are abundant in sunflower calathide, especially evaluated for the inhibition of xanthine oxidase (XO) and the expression levels of urate transporters with SCHE. Molecular docking of a chlorogenic acid–XO complex was more stable than that of the Scopoletin–XO, and its binding pockets, which closed the Mo = S center, was similar to xanthine pockets. Moreover, chlorogenic acid exhibited stronger inhibition than that of the scopoletin below 260 μM, despite the IC50 of scopoletin (577.7 μM) being lower than that chlorogenic acid (844.7 μM) on the UA generation assessed by a spectrophotometer in vitro. It revealed that chlorogenic acid and scopoletin were competitive inhibitors of XO. In addition, the SCHE (300 μg/mL) and chlorogenic acid (0.75 mM) obviously inhibited urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) expression levels, while scopoletin significantly upregulated the expression of GLUT9. To summarize, chlorogenic acid served a crucial role in UA regulation consistent with the SCHE and functioned as an important ingredient of SCHE. The strategic analysis of SCHE combined with scopoletin and chlorogenic acid may contribute to the development of food supplemental alternatives on UA metabolism and the reduction of agricultural byproduct waste.
Research Status of Pathogenesis of Pneumoconiosis and Dust Control Technology in Mine—A Review
Authors: Liu Guoming , Xu Qianqian , Zhao Jipeng , Nie Wen , Guo Qingkun , Ma Guanguo
Published: November 2021

Pneumoconiosis has become one of the biggest threats to the occupational health and life safety of mining workers in China. The number of pneumoconiosis cases has continued to rise in recent years. The main task of occupational health development is to study the pathogenesis of pneumoconiosis and to develop mine dust prevention and control technology. Therefore, this paper summarizes the research progress of coal worker pneumoconiosis and dust prevention and control in mines. Firstly, the research progress of coal worker pneumoconiosis is analyzed from the aspects of pathogenesis, animal model and pathological changes of coal worker pneumoconiosis. Then, the existing basic theory and technology of dust prevention are described, including ventilation and dust removal, spray and dust suppression, and chemical dust suppression methods. Finally, based on the dust removal theory of wet shotcrete, the progress of shotcrete dust control technology and equipment used for shotcrete is summarized from the aspects of shotcrete technology process and shotcrete materials. At the same time, in view of the shortcomings of the existing research, the next research prospect is given in the pathogenesis of pneumoconiosis, intelligent dust prevention, jet spraying dust removal and so on. This paper provides theoretical support for realizing the separate source and efficient treatment of mine dust control and helps to improve the clean production level of mine, control and prevent pneumoconiosis.
Determination of Sugar, pH, and Anthocyanin Contents in Port Wine Grape Berries through Hyperspectral Imaging: An Extensive Comparison of Linear and Non-Linear Predictive Methods
Authors: Gomes Véronique , Rendall Ricardo , Reis Marco Seabra , Mendes-Ferreira Ana , Melo-Pinto Pedro
Published: November 2021

This paper presents an extended comparison study between 16 different linear and non-linear regression methods to predict the sugar, pH, and anthocyanin contents of grapes through hyperspectral imaging (HIS). Despite the numerous studies on this subject that can be found in the literature, they often rely on the application of one or a very limited set of predictive methods. The literature on multivariate regression methods is quite extensive, so the analytical domain explored is too narrow to guarantee that the best solution has been found. Therefore, we developed an integrated linear and non-linear predictive analytics comparison framework (L&NL-PAC), fully integrated with five preprocessing techniques and five different classes of regression methods, for an effective and robust comparison of all alternatives through a robust Monte Carlo double cross-validation stratified data splitting scheme. L&NLPAC allowed for the identification of the most promising preprocessing approaches, best regression methods, and wavelengths most contributing to explaining the variability of each enological parameter for the target dataset, providing important insights for the development of precision viticulture technology, based on the HSI of grape. Overall, the results suggest that the combination of the Savitzky−Golay first derivative and ridge regression can be a good choice for the prediction of the three enological parameters.
A Self-Adaptive-Coefficient-Double-Power Sliding Mode Control Method for Lower Limb Rehabilitation Exoskeleton Robot
Authors: Zhang Yuepeng , Cao Guangzhong , Li Wenzhou , Chen Jiangcheng , Li Linglong , Diao Dongfeng
Published: November 2021

Lower limb rehabilitation exoskeleton robots have the characteristics of nonlinearity and strong coupling, and they are easily disturbed during operation by environmental factors. Thus, an accurate dynamic model of the robot is difficult to obtain, and achieving trajectory tracking control of the robot is also difficult. In this article, a self-adaptive-coefficient double-power sliding mode control method is proposed to overcome the difficulty of tracking the robot trajectory. The method combines an estimated dynamic model with sliding mode control. A nonlinear control law was designed based on the robot dynamics model and computational torque method, and a compensation term of control law based on double-power reaching law was introduced to reduce the disturbance from model error and environmental factors. The self-adaptive coefficient of the compensation term of the control law was designed to adaptively adjust the compensation term to improve the anti-interference ability of the robot. The simulation and experiment results show that the proposed method effectively improves the trajectory tracking accuracy and anti-interference ability of the robot. Compared with the traditional computed torque method, the proposed method decreases the tracking error by more than 71.77%. The maximum absolute error of the hip joint and knee joint remained below 0.55° and 1.65°, respectively, in the wearable experiment of the robot.
An Experimental Study on State Representation Extraction for Vision-Based Deep Reinforcement Learning
Authors: Ren Junkai , Zeng Yujun , Zhou Sihang , Zhang Yichuan
Published: November 2021

Scaling end-to-end learning to control robots with vision inputs is a challenging problem in the field of deep reinforcement learning (DRL). While achieving remarkable success in complex sequential tasks, vision-based DRL remains extremely data-inefficient, especially when dealing with high-dimensional pixels inputs. Many recent studies have tried to leverage state representation learning (SRL) to break through such a barrier. Some of them could even help the agent learn from pixels as efficiently as from states. Reproducing existing work, accurately judging the improvements offered by novel methods, and applying these approaches to new tasks are vital for sustaining this progress. However, the demands of these three aspects are seldom straightforward. Without significant criteria and tighter standardization of experimental reporting, it is difficult to determine whether improvements over the previous methods are meaningful. For this reason, we conducted ablation studies on hyperparameters, embedding network architecture, embedded dimension, regularization methods, sample quality and SRL methods to compare and analyze their effects on representation learning and reinforcement learning systematically. Three evaluation metrics are summarized, including five baseline algorithms (including both value-based and policy-based methods) and eight tasks are adopted to avoid the particularity of each experiment setting. We highlight the variability in reported methods and suggest guidelines to make future results in SRL more reproducible and stable based on a wide number of experimental analyses. We aim to spur discussion about how to assure continued progress in the field by minimizing wasted effort stemming from results that are non-reproducible and easily misinterpreted.
Determination of Local Stresses and Strains within the Notch Strain Approach: Efficient Implementation of Notch Root Approximations
Authors: Burghardt Ralf , Masendorf Lukas , Wächter Michael , Esderts Alfons
Published: November 2021

An estimation of the elastic-plastic stress state using elasticity-theoretical input data is an essential part of the service life estimation with the local strain approach in general and a German guideline based on it, in particular. This guideline uses two different notch root approximations (an extended version of Neuber’s rule and an approach according to Seeger and Beste) for this estimation. Both require the implementation of Newton’s method to be iteratively solved. However, many options are left open to the user concerning implementation in program code. This paper discusses ways in which notch root approximation methods can be implemented efficiently for use in software systems and elaborates an application recommendation. The following aspects and their influence on the computational accuracy and performance of Newton’s method are considered in detail: influence of the formulation of the root finding problem, determination of the derivative required for Newton’s method and influence of the termination criterion. The investigation shows that the advice given in the abovementioned guideline indeed leads to a conservative implementation. By carefully considering the investigated aspects, however, the computational performance can be increased by approximately a factor of 2–3 without influencing the accuracy of the service life estimation.
Open Tools for Analysis of Elements Related to Public Transport Performance. Case Study: Tram Network in Bucharest
Authors: Andrei Liliana , Luca Oana
Published: November 2021

The present paper aims to be useful for public transport operators and municipalities, as it should enable them to make decisions and to optimize public transport schedules during peak hours. In this study, we outline the data and the means necessary for the creation and use of a specific database for a dynamic spatial analysis of the public transportation network. This will facilitate the analysis of public transport vehicle operating programs and the simulation of new transport programs using open-source software. This paper delivers the first digital map of the public transport in Bucharest. Using the QGIS software and the PostgresSQL database, (i) we analyzed the accessibility of public transport stops for residential areas (5-min isochrones, corresponding to walking distances of 400 m), and (ii) we determined the correlation of transport vehicle programs with the existing transport network to optimize the headway of vehicles. These two elements were considered for the analysis of public transport performance. The research study was based on the tram network in Bucharest, but it can be easily upscaled for the entire public transport network and may be replicated in other large cities.
Oxadiazolyl-Pyridinium as Cationic Scaffold for Fluorinated Ionic Liquid Crystals
Authors: Weber Melina S. , Schulze Margit , Lazzara Giuseppe , Palumbo Piccionello Antonio , Pace Andrea , Pibiri Ivana
Published: November 2021

The synthesis and characterization of a new class of 1,2,4-oxadiazolylpyridinium as a cationic scaffold for fluorinated ionic liquid crystals is herein described. A series of 12 fluorinated heterocyclic salts based on a 1,2,4-oxadiazole moiety, connected through its C(5) or C(3) to an N-alkylpyridinium unit and a perfluoroheptyl chain, differing in the length of the alkyl chain and counterions, has been synthesized. As counterions iodide, bromide and bis(trifluoromethane)sulfonimide have been considered. The synthesis, structure, and liquid crystalline properties of these compounds are discussed on the basis of the tuned structural variables. The thermotropic properties of this series of salts have been investigated by differential scanning calorimetry and polarized optical microscopy. The results showed the existence of an enantiotropic mesomorphic smectic liquid crystalline phase for six bis(trifluoromethane)sulfonimide salts.
Elliptic Problems with Additional Unknowns in Boundary Conditions and Generalized Sobolev Spaces
Authors: Anop Anna , Chepurukhina Iryna , Murach Aleksandr
Published: November 2021

In generalized inner product Sobolev spaces we investigate elliptic differential problems with additional unknown functions or distributions in boundary conditions. These spaces are parametrized with a function OR-varying at infinity. This characterizes the regularity of distributions more finely than the number parameter used for the Sobolev spaces. We prove that these problems induce Fredholm bounded operators on appropriate pairs of the above spaces. Investigating generalized solutions to the problems, we prove theorems on their regularity and a priori estimates in these spaces. As an application, we find new sufficient conditions under which components of these solutions have continuous classical derivatives of given orders. We assume that the orders of boundary differential operators may be equal to or greater than the order of the relevant elliptic equation.
Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
Authors: Zhang Xinbo , Ma Quan , Li Fujian , Ding Yonggang , Yi Yuan , Zhu Min , Ding Jinfeng , Li Chunyan , Guo Wenshan , Zhu Xinkai
Published: November 2021

The development of crop cultivars with high nitrogen use efficiency (NUE) under low-N fertilizer inputs is imperative for sustainable agriculture. However, there has been little research on the molecular mechanisms underlying enhanced resilience to low N in high-NUE plants. The comparison of the transcriptional responses of genotypes contrasting for NUE will facilitate an understanding of the key molecular mechanism of wheat resilience to low-N stress. In the current study, the RNA sequencing (RNA-seq) technique was employed to investigate the genotypic difference in response to N deficiency between two wheat NILs (1Y, high-NUE, and 1W, low-NUE). In our research, high- and low-NUE wheat NILs showed different patterns of gene expression under N-deficient conditions, and these N-responsive genes were classified into two major classes, including “frontloaded genes” and “relatively upregulated genes”. In total, 103 and 45 genes were identified as frontloaded genes in high-NUE and low-NUE wheat, respectively. In summary, our study might provide potential directions for further understanding the molecular mechanism of high-NUE genotypes adapting to low-N stress.
Long-Term Consequences of Placental Vascular Pathology on the Maternal and Offspring Cardiovascular Systems
Authors: Benagiano Marisa , Mancuso Salvatore , Brosens Jan J. , Benagiano Giuseppe
Published: November 2021

Over the last thirty years, evidence has been accumulating that Hypertensive Disorders of Pregnancy (HDP) and, specifically, Preeclampsia (PE) produce not only long-term effects on the pregnant woman, but have also lasting consequences for the fetus. At the core of these consequences is the phenomenon known as defective deep placentation, being present in virtually every major obstetrical syndrome. The profound placental vascular lesions characteristic of this pathology can induce long-term adverse consequences for the pregnant woman’s entire arterial system. In addition, placental growth restriction and function can, in turn, cause a decreased blood supply to the fetus, with long-lasting effects. Women with a history of HDP have an increased risk of Cardiovascular Diseases (CVD) compared with women with normal pregnancies. Specifically, these subjects are at a future higher risk of: Hypertension; Coronary artery disease; Heart failure; Peripheral vascular disease; Cerebrovascular accidents (Stroke); CVD-related mortality. Vascular pathology in pregnancy and CVD may share a common etiology and may have common risk factors, which are unmasked by the “stress” of pregnancy. It is also possible that the future occurrence of a CVD may be the consequence of endothelial dysfunction generated by pregnancy-induced hypertension that persists after delivery. Although biochemical and biophysical markers of PE abound, information on markers for a comparative evaluation in the various groups is still lacking. Long-term consequences for the fetus are an integral part of the theory of a fetal origin of a number of adult diseases, known as the Barker hypothesis. Indeed, intrauterine malnutrition and fetal growth restriction represent significant risk factors for the development of chronic hypertension, diabetes, stroke and death from coronary artery disease in adults. Other factors will also influence the development later in life of hypertension, coronary and myocardial disease; they include parental genetic disposition, epigenetic modifications, endothelial dysfunction, concurrent intrauterine exposures, and the lifestyle of the affected individual.
Cutting Edge Research for Exploration of Biomolecules for Gemcitabine-Based Chemo-Resistant Advanced Bile Duct Cancer: From Basic Study to Clinical Trial
Authors: Wu Chiao-En , Yeh Chun-Nan
Published: November 2021

Bile duct cancer (BDC) has been identified as a highly aggressive cancer arising from epithelial cells of the bile duct, including intrahepatic, perihilar, and extrahepatic [...]
Urinary Volatiles and Chemical Characterisation for the Non-Invasive Detection of Prostate and Bladder Cancers
Authors: Tyagi Heena , Daulton Emma , Bannaga Ayman S. , Arasaradnam Ramesh P. , Covington James A.
Published: November 2021

Bladder cancer (BCa) and prostate cancer (PCa) are some of the most common cancers in the world. In both BCa and PCa, the diagnosis is often confirmed with an invasive technique that carries a risk to the patient. Consequently, a non-invasive diagnostic approach would be medically desirable and beneficial to the patient. The use of volatile organic compounds (VOCs) for disease diagnosis, including cancer, is a promising research area that could support the diagnosis process. In this study, we investigated the urinary VOC profiles in BCa, PCa patients and non-cancerous controls by using gas chromatography-ion mobility spectrometry (GC-IMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) to analyse patient samples. GC-IMS separated BCa from PCa (area under the curve: AUC: 0.97 (0.93–1.00)), BCa vs. non-cancerous (AUC: 0.95 (0.90–0.99)) and PCa vs. non-cancerous (AUC: 0.89 (0.83–0.94)) whereas GC-TOF-MS differentiated BCa from PCa (AUC: 0.84 (0.73–0.93)), BCa vs. non-cancerous (AUC: 0.81 (0.70–0.90)) and PCa vs. non-cancerous (AUC: 0.94 (0.90–0.97)). According to our study, a total of 34 biomarkers were found using GC-TOF-MS data, of which 13 VOCs were associated with BCa, seven were associated with PCa, and 14 VOCs were found in the comparison of BCa and PCa.
Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
Authors: Bocheńska Katarzyna , Moskot Marta , Gabig-Cimińska Magdalena
Published: November 2021

Psoriasis (Ps), commonly perceived as a skin and joint disorder, has a complex basis and results from disturbances in the sophisticated network between skin and the immune system. This makes it difficult to properly depict the complete pathomechanism on an in vitro scale. Deciphering the complicated or even subtle modulation of intra- and intercellular factors, assisted by the implementation of in vitro human skin models, may provide the opportunity to dissect the disease background step by step. In addition to reconstructed artificial skin substitutes, which mimic the native physiological context, in vitro models are conducive to the broad “3 Rs” philosophy (reduce, refine, and replace) and represent important tools for basic and applied skin research. To meet the need for a more comprehensive in vitro Ps model, a set of various experimental conditions was applied in this study. The selection of in vitro treatment that mimicked the Ps phenotype was illustrated by analyses of discriminating biomarker genes involved in the pathogenesis of the disease, i.e., keratinocyte differentiation markers, antimicrobial peptides, chemokines, and proliferation markers. This resulted in a reproducible protocol for the use of the primary skin keratinocyte (pKC) monoculture treated with a cytokine cocktail (5MIX, i.e., interleukin (IL) 1 alpha (IL-1α), IL-17A, IL-22, oncostatin M (OSM), and tumour necrosis factor alpha (TNF-α)) at a calcium (Ca2+) concentration (i.e., 2 mM) in an applied medium, which best mirrored the in vitro Ps-like inflammatory model. In addition, based on waste skin material, the method has the potential for extensive experimentation, both in detailed molecular studies and preclinical tests.
Significance of Interleukin (IL)-4 and IL-13 in Inflammatory Arthritis
Authors: Iwaszko Milena , Biały Sylwia , Bogunia-Kubik Katarzyna
Published: November 2021

Interleukin (IL)-4 and IL-13 belong to the T helper 2 (Th2) cytokine family, along with IL-3, IL-5, and IL-9. These cytokines are key mediators of allergic inflammation. They have important immunomodulatory activities and exert influence on a wide variety of immune cells, such as B cells, eosinophils, basophils, monocytes, fibroblasts, endothelial cells, airway epithelial cells, smooth muscle cells, and keratinocytes. Recent studies have implicated IL-4 and IL-13 in the development of various autoimmune diseases. Additionally, these cytokines have emerged as potential players in pathogenesis of inflammatory arthritis. Recent findings suggest that the IL-4 and IL-13 might play a significant role in the downregulation of inflammatory processes underlying RA pathology, and beneficially modulate the course of the disease. This review summarizes the biological features of the IL-4 and IL-13 and provides current knowledge regarding the role of these cytokines in inflammatory arthritis.