bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Term Recidivism of Mentally Disordered Offenders Considered “Dangerous to the Public” in Switzerland
Authors: Schaffner Daniela , Weber Michael , Kochuparackal Tanya , Graf Marc , Hachtel Henning
Published: April 2021

Objectives:The commissions for risk assessment of offenders dangerous to the public were established in 1995 in Switzerland. The main goal was to reduce recidivism of offenders released into the community by means of identifying high-risk offenders and recommending measures for offender management. This study investigates long-term recidivism data of this high-risk cohort of offenders.

Methods:Baseline data included risk assessment of one of the commissions, the type of index offense, and psychiatric disorders according to ICD-10 for the total cohort of offenders examined by the commissions between 1995 and 2009. Criminal records were drawn in 2019 for all offenders from the Swiss Federal Office of Justice.

Results:From a total of 147 offenders 35 recidivated within a median time at risk of 9.1 years (31.8%), of which 10 (9.1%) recommitted a severe offense. Within the treatment status, sentences (imprisonment and preventive detention) were compared to court-ordered measures (in- or outpatient court ordered treatment, civil court mandated treatment, vocational training facility). There were no significant differences comparing treatment status, different diagnostic groups, type of index offense and other risk factors. Except of age at release (or relapse), which predicted recidivism with younger subjects showing higher recidivism rates (p= 0.014).

Conclusion:Our study showed that over a long-term time at risk this high-risk cohort showed a similar recidivism rate as many other studies with different cohorts. With appropriate management recidivism rates in high-risk offenders can be lowered allowing them being consecutively reintegrated into society. The finding that younger subjects have higher recidivism rate was reproduced in this population.
Convergence of Precision Medicine and Public Health Into Precision Public Health: Toward a Big Data Perspective
Authors: Velmovitsky Pedro Elkind , Bevilacqua Tatiana , Alencar Paulo , Cowan Donald , Morita Plinio Pelegrini
Published: April 2021

The field of precision medicine explores disease treatments by looking at genetic, socio-environmental, and clinical factors, thus trying to provide a holistic view of a person's health. Public health, on the other hand, is focused on improving the health of populations through preventive strategies and timely interventions. With recent advances in technology, we are able to collect, analyze and store for the first-time large volumes of real-time, diverse and continuous health data. Typically, the field of precision medicine deals with a huge amount of data from few individuals; public health, on the other hand, deals with limited data from a population. With the coming of Big Data, the fields of precision medicine and public health are converging into precision public health, the study of biological and genetic factors supported by large amounts of population data. In this paper, we explore through a comprehensive review the data types and use cases found in precision medicine and public health. We also discuss how these data types and use cases can converge toward precision public health, as well as challenges and opportunities provided by research and analyses of health data.
Economic Evaluation of First-Line Atezolizumab for Extensive-Stage Small-Cell Lung Cancer in the US
Authors: Wang Yingcheng , Rui Mingjun , Yang Lan , Wang Xintian , Shang Ye , Ma Aixia , Li Hongchao
Published: April 2021

Introduction: This study evaluated the cost-effectiveness of atezolizumab + chemotherapy vs. chemotherapy as first-line treatment for extensive-stage small-cell lung cancer (SCLC) in the United States (US).

Methods: The three health states partitioned survival (PS) model was used over the lifetime. Effectiveness and safety data were derived from the IMpower133 trial. The parametric survival model and mixture cure model were used for the atezolizumab + chemotherapy group to explore the long-term uncertainty of the effect of immunotherapy, and the parametric survival model was used for the chemotherapy group. Costs were derived from the pricing files of Medicare and Medicaid Services, and utility values were derived from previous studies. Sensitivity analyses were performed to observe model stability.

Results: If the mixture cure model was considered for the intervention group, compared with chemotherapy alone, atezolizumab + chemotherapy yielded an additional 0.11 quality-adjusted life-years (QALYs), with an incremental cost of US$84,257. The incremental cost-utility ratio (ICUR) was US$785,848/QALY. If the parametric survival model was considered for the intervention group, atezolizumab + chemotherapy yielded an additional 0.10 QALYs, with an incremental cost of US$84,257; the ICUR was US$827,610/QALY. In the one-way sensitivity analysis, progression-free (PF) and postprogression (PP) utilities were the main drivers. In the scenario analysis (PF utility = 0.673, PP utility = 0.473), the results showed that the ICUR was US$910,557/QALY and US$965,607/QALY when the mixture cure model and parametric survival model was considered for the intervention group, respectively. In the PSA, the probabilities that atezolizumab + chemotherapy would not be cost-effective were 100% if the willingness-to-pay threshold was US$100,000/QALY.

Conclusions: The findings of the present analysis suggest that atezolizumab + chemotherapy is not cost-effective in patients receiving first-line treatment for extensive-stage SCLC in the US.
Irrigation-Intensive Groundwater Modeling of Complex Aquifer Systems Through Integration of Big Geological Data
Authors: Vahdat-Aboueshagh Hamid , Tsai Frank T.-C. , Bhatta Dependra , Paudel Krishna P.
Published: April 2021

This study identifies hydrogeologic characteristics of complex aquifers based on constructing stratigraphic structure with large, non-uniform well log data. The approach was validated through a modeling study of the irrigation-intensive Chicot aquifer system, which is an important Pleistocene-Holocene aquifer of the Coastal Lowlands aquifer system in the southwestern Louisiana. Various well log types were unified into the same data structure, prioritized based on data sources, and interpolated to generate a detailed stratigraphic structure. More than 29,000 well logs were integrated to construct a stratigraphy model of 56 model layers for the Chicot aquifer system. The stratigraphy model revealed interconnections of various sands in the system, where 90% of the model domain is covered by fine-grained sediments. Although the groundwater model estimated a slight groundwater storage gain during 2005–2014 for the entire region, groundwater storage in the agricultural area was depleted. Nevertheless, the quick groundwater storage recovery during the non-irrigation seasons suggests that the Chicot aquifer system is a prolific aquifer system. The groundwater modeling result shows that the gulfward groundwater flow direction prior to pumping has been reversed toward inland pumping areas. The large upward vertical flow from the deeper sands indicates potential saltwater migration from the base of the Chicot aquifer system.
Protein—Not Just a Food Group!
Authors: Batalis Stephanie , Hollis Thomas
Published: April 2021

Eating protein is important to help us grow strong and stay healthy. However, protein is more than just a food group! Proteins are the workers inside of our busy cells. These proteins have some amazing jobs to do, and thousands of proteins work together every day to keep our bodies healthy. In this article, we will discuss how proteins are made and why we need protein to keep our bodies running smoothly.
Emotions, Emotion Management and Emotional Intelligence in the Workplace: Healthcare Professionals' Experience in Emotionally-Charged Situations
Authors: Carminati Lara
Published: April 2021

This perspective article is grounded in a cognitive and context-dependent view on emotions. By considering emotions as socially embedded and constructed, the different but related concepts of Emotion Management and Emotional Intelligence can be introduced. Yet, research juxtaposing and applying them within the healthcare sector to explain healthcare professionals' multifaceted emotional experiences at work is still scarce. Hence, this article contributes to the literature on emotions by offering an overarching perspective on how the juxtaposition of Emotion Management and Emotional Intelligence may help healthcare professionals to bridge the developmental transition between these two crucial abilities which, in turn, can help them overcome emotional difficulties in complex situations. Such integration would positively influence individuals' behavioral and mental health, as well as the overall quality of the healthcare system.
Integrating Digital Technologies to Aid Grassland Productivity and Sustainability
Authors: Stevens David R. , Thompson Bryan R. , Johnson Patricia , Welten Brendon , Meenken Esther , Bryant Jeremy
Published: April 2021

Digital technologies provide an opportunity to further increase the sustainability and productivity of grasslands and rangelands. Three resources are key to that change. These are the soil on which forage grows, the forages that grow on those soils and the animals that use the forage resource as food. This paper describes elements of technologies to measure and monitor these resources and provides some insights on combining that knowledge and controlling the animal's utilization through virtual fencing. There are many potential challenges to the application of digital technologies to pastoral farming. These often require the calibration of digital signals to define biophysical characteristics. The significant repository of historic data of pasture growth over many geo-climatic regions, for example, provides New Zealand with an opportunity to accelerate that development. Future advances in rangeland use, nutrient deposition, greenhouse gas emissions and the provision and utilization of high quality and quantity will be enabled by the application of digital technologies at scale, under the control of virtual fencing. Digital technologies may provide the means to maintain or enhance ruminant production from grassland in a sustainable operating space into the future.
Limited Economic-Ecological Trade-Offs in a Shifting Agricultural Landscape: A Case Study From Kern County, California
Authors: Wartenberg Ariani C. , Moanga Diana , Potts Matthew D. , Butsic Van
Published: April 2021

Increasing global food production and livelihoods while maintaining ecosystem health will require significant changes in the way existing farming landscapes are managed. To this end, developing a systemic understanding of the economic and ecological impacts of different cropping systems, and identifying trade-offs and synergies between them, is crucial to inform decision-making for policy makers and landowners. Here, we investigate the impacts of agricultural land-use change for 15 distinct crops in Kern County, California, by looking at spatial and temporal changes in ecosystem indicators. We focus our analysis on three agricultural ecosystem pressures (water use, soil erosion, and pesticide use) and three agricultural ecosystem services (profits, calorie production, and C sequestration). Between 2002 and 2018, agriculture in Kern County underwent a shift from annual row crop to nut tree crop production. At the landscape-scale, we found high increases in ecosystem service provision (total profits, calorie production, and annual C sequestration increased by 105, 29, and 37%, respectively), coupled with smaller changes in ecosystem pressures (total soil erosion and evapotranspiration increased by 10 and 5%, respectively, and total pesticide use declined by 4%). We identified no salient trade-offs or synergies among crops. Our results illustrate that in the highly productive agricultural hotspot of Kern County, a combination of changes in land-cover allocation or land-use efficiency may have mitigated stronger negative environmental impacts following a broad shift from annual to perennial crops.
Protected Cultivation of Horticultural Crops in Uttarakhand: An Economic Analysis
Authors: Kumar Pramod , Kar Amit , Singh Dharam Raj , Perumal Anbukkani , Shivamurthy Satish Gowda Chirathahalli , Reddy Krishna Viswanatha , Badal Prakash Singh , Lala Kamble Ankush , Kamalvanshi Virendra , Jha Girish Kumar , Nain Manjeet Singh , Pachiyappan Prakash , Alataway Abed , Dewidar Ahmed , Elansary Hosam O.
Published: April 2021

In recent times, with the globalization of markets, shrinking of land and climate change, food basket diversification, increase in demand for nutrient-rich food, the protected cultivation of high-value crops (HVCs) have assumed a pivotal role in augmenting higher crop productivity and profitability and enhancing nutritional security of the growing population. In this context, a study was undertaken to analyze the impact of protected cultivation in horticultural crops in the districts of Almora and Dehradun in the Uttarakhand state. It was mainly based on primary data obtained through a primary survey and focus group discussion with the 96 farmers practicing protected cultivation by using a well-structured and pre-tested questionnaire. In economic analysis, the project analysis tools were used to assess the feasibility of the protected cultivation. The study clearly demonstrated that the cultivation of vegetables and flowers under protected cultivation is a highly profitable enterprise. However, the findings of the study indicated that the subsidy scheme needs to be continued to encourage maximum farmers to adopt protected cultivation and farmers need to be encouraged to form farmers producers organizations (FPOs), which would help them in seeking better quality of inputs and enhancing negotiating power in the market to realize maximum returns for their farm produce.
Bio-Based Value Chains Potential in the Management of Cacao Pod Waste in Colombia, a Case Study
Authors: Meza-Sepúlveda Diana C. , Castro Ana M. , Zamora Annie , Arboleda Jorge W. , Gallego Adriana M. , Camargo-Rodríguez Anyela V.
Published: April 2021

Agriculture generates 11.4 billion tons of biomass worldwide, including residues from crop production and industrial processing. Improper disposal of agricultural residues results in environmental pollution and the waste of valuable biomass resources. Management of agricultural waste is particularly suboptimal in developing countries where low added-value traditional practices to manage the residues are commonplace. A bioeconomy approach to better manage agricultural waste is to use renewable biological resources from land and sea to produce new materials and energy, allowing the development of bio-based value chains (BBVC). This study explored the potential of BBVC in the management of cacao pod husk (CPH), the primary residual biomass generated from the production of cacao in Colombia. Thus, a literature review on cacao pod husk (CPH) management strategies and a survey for farmers from Caldas State in Colombia between August and November of 2020 were performed to identify the potentials and limitations of BBVC in the management of CPH. Assessment of CPH management strategies suggested variable uses for CPH categorized in energy, food, and miscellaneous. Analysis of surveys indicated farmers are keen to implement strategies to better manage their agricultural waste, but that information is not available to them. Finally, an approach to develop a BBVC from cacao was proposed, which we plan to implement as a future research direction. We expect to impact the economic growth positively in the region with bio-based products in the market.
Assessing the Impact of Rice Cultivation and Off-Season Period on Dynamics of Soil Enzyme Activities and Bacterial Communities in Two Agro-Ecological Regions of Mozambique
Authors: Ezeokoli Obinna T. , Nuaila Valter N. A. , Obieze Chinedu C. , Muetanene Belo A. , Fraga Irene , Tesinde Maria Natalia , Ndayiragije Alexis , Coutinho João , Melo Ana M. P. , Adeleke Rasheed A. , Ribeiro-Barros Ana I. , Fangueiro David
Published: April 2021

Soil ecosystem perturbation due to agronomic practices can negatively impact soil productivity by altering the diversity and function of soil health determinants. Currently, the influence of rice cultivation and off-season periods on the dynamics of soil health determinants is unclear. Therefore, soil enzyme activities (EAs) and bacterial community compositions in rice-cultivated fields at postharvest (PH) and after a 5-month off-season period (5mR), and fallow-fields (5-years-fallow, 5YF; 10-years-fallow, 10YF and/or one-year-fallow, 1YF) were assessed in two agroecological regions of Mozambique. EAs were mostly higher in fallow fields than in PH, with significant (p < 0.05) differences detected for β-glucosidase and acid phosphatase activities. Only β-glucosidase activity was significantly (p < 0.05) different between PH and 5mR, suggesting that β-glucosidase is responsive in the short-term. Bacterial diversity was highest in rice-cultivated soil and correlated with NO3−, NH4+ and electrical conductivity. Differentially abundant genera, such as Agromyces, Bacillus, Desulfuromonas, Gaiella, Lysobacter, Micromonospora, Norcadiodes, Rubrobacter, Solirubrobacter and Sphingomonas were mostly associated with fallow and 5mR fields, suggesting either negative effects of rice cultivation or the fallow period aided their recovery. Overall, rice cultivation and chemical parameters influenced certain EAs and shaped bacterial communities. Furthermore, the 5-month off-season period facilitates nutrient recovery and proliferation of plant-growth-promoting bacteria.
Assessment of Drought and Heat Tolerance of Durum Wheat Lines Derived from Interspecific Crosses Using Physiological Parameters and Stress Indices
Authors: Aberkane Hafid , Belkadi Bouchra , Kehel Zakaria , Filali-Maltouf Abdelkarim , Tahir Izzat S. A. , Meheesi Sara , Amri Ahmed
Published: April 2021

Drought and high temperature are the major abiotic stresses for wheat production. The present study investigated the effect of drought and chronic heat stress on physiological parameters of durum wheat lines derived from interspecific crosses and their association with yield. Seventy-seven durum wheat lines were evaluated during two seasons (2016–2017 and 2017–2018) for drought tolerance at Tessaout (Morocco) using irrigated and rainfed treatments and for heat tolerance at Wad Medani (Sudan). Five drought screening indices (alone or combined) and physiological parameters were used to assess drought and heat tolerance. Among the physiological parameters used, canopy temperature (CT) had moderate heritability and was significantly affected by both severe and moderate drought stresses. CT at early heading showed a stronger correlation with grain yield (GY) and total biomass (BY) under heat stress. The use of maximum quantum yield of PSII (Fv/Fm) for drought/heat screening was limited by the low genetic variation despite its significant correlation with yield under drought (r2 = 0.22) and heat (r2 = 0.4). The normalized difference vegetation index (NDVI) at vegetative stage was highly correlated with GY and BY and it showed high genotypic variation that can allow for efficient selection. The grain filling rate (GFR) was found to be highly correlated with GY and BY under heat stress. The modified stress tolerance index (MsSTI) had the highest association with GY under drought (R2 = 0.82) while the mean productivity (MP) was adapted to both optimal conditions (R2 = 0.77) and drought stress (R2 = 0.73). The computation of a mean score index (MSI) improved the selection efficiency under drought (R2 = 0.92). The results showed good potential for lines derived from wide crosses to increase variability for heat and drought adaptive physiological traits.
Changes in the Relationship between Ionized and Total Calcium in Clinically Healthy Dairy Cows in the Period around Calving
Authors: Ott Dorothee , Schrapers Katharina T. , Aschenbach Jörg R.
Published: April 2021

We aimed to establish a model for prediction of iCa from tCa, using multivariable regressions with diverse blood constituents. Blood was taken from 14 cows at days −2, 0, 2, 4, 7, and 14 relative to parturition. Cows were clinically healthy, and no hypocalcaemia prophylaxis and treatment were applied. Total calcium and further parameters were determined from frozen serum. Ionized calcium, blood gases, and electrolytes were determined from heparin-stabilized blood samples. Linear regression between iCa and tCa was estimated. Precision improved only slightly using a multivariable model. Best precision was achieved when estimating the iCa:tCa ratio from other blood constituents. To identify the reason behind the poorly predictive value of tCa for iCa, the relative changes of iCa and tCa around calving were calibrated to the respective values of day −2 (=100%) for each cow. An increase in the iCa:tCa ratio was observed from 0.43 at day −2 to 0.48 at day 0, followed by a gradual decrease towards 0.43 at day 7. We conclude that routine measurement of iCa should be implemented in the diagnosis of hypocalcaemia. An optimized estimate of iCa from tCa with non-esterified fatty acids (NEFA), beta-hydroxybutyric acid, cholesterol, and phosphorous as co-predictors is still poorly satisfying.
Antibiotic Resistance in Pediatric Infections: Global Emerging Threats, Predicting the Near Future
Authors: Romandini Alessandra , Pani Arianna , Schenardi Paolo Andrea , Pattarino Giulia Angela Carla , De Giacomo Costantino , Scaglione Francesco
Published: April 2021

Antibiotic resistance is a public health threat of the utmost importance, especially when it comes to children: according to WHO data, infections caused by multidrug resistant bacteria produce 700,000 deaths across all ages, of which around 200,000 are newborns. This surging issue has multipronged roots that are specific to the pediatric age. For instance, the problematic overuse and misuse of antibiotics (for wrong diagnoses and indications, or at wrong dosage) is also fueled by the lack of pediatric-specific data and trials. The ever-evolving nature of this age group also poses another issue: the partly age-dependent changes of a developing system of cytochromes determine a rather diverse population in terms of biochemical characteristics and pharmacokinetics profiles, hard to easily codify in an age- or weight-dependent dosage. The pediatric population is also penalized by the contraindications of tetracyclines and fluoroquinolones, and by congenital malformations which often require repeated hospitalizations and pharmacological and surgical treatments from a very young age. Emerging threats for the pediatric age are MRSA, VRSA, ESBL-producing Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae and the alarming colistin resistance. Urgent actions need to be taken in order to step back from a now likely post-antibiotic era, where simple infections might cause infant death once again.
Combined Ubisol-Q10 and Ashwagandha Root Extract Target Multiple Biochemical Mechanisms and Reduces Neurodegeneration in a Paraquat-Induced Rat Model of Parkinson’s Disease
Authors: Vegh Caleb , Wear Darcy , Okaj Iva , Huggard Rachel , Culmone Lauren , Eren Sezen , Cohen Jerome , Rishi Arun K. , Pandey Siyaram
Published: April 2021

Parkinson’s disease (PD) is characterized by progressive neurodegeneration in the substantia nigra (SN) region resulting in loss of movement coordination. Current therapies only provide symptomatic relief, and there is no agent to halt the progression of PD. Previously, Ubisol-Q10, a water-soluble formulation of coenzyme-Q10, and ethanolic root extract of ashwagandha (ASH) have been shown to inhibit PD pathology in rodent models when used alone. Here, we evaluated the neuroprotective efficacy of oral administration of ASH and Ubisol-Q10 alone and in combination in a paraquat-induced PD rat model. The combined treatment resulted in better-preserved neuron morphology compared to Ubsiol-Q10 or ASH alone. The combination treatment enhanced activation of pro-survival astroglia and inhibited pro-inflammatory microglia. While anti-oxidative effects were seen with both agents, Ubisol-Q10 activated autophagy, whereas ashwagandha showed a better anti-inflammatory response. Thus, the combined treatment caused inhibition of oxidative stress, autophagy activation, inhibition of pro-inflammatory microglia, and activation of pro-survival astroglia. Consequently, paraquat (PQ)-treated rats given the combination treatment in drinking water did not show motor impairment. Based on these interesting observations, the combined treatment containing two well-tolerated natural compounds could be a more effective strategy to halt the progression of PD.
Influence of Traditional and Solar Reflective Coatings on the Heat Transfer of Building Roofs in Mexico
Authors: Hernández-Pérez Iván
Published: April 2021

Building roofs are sources of unwanted heat for buildings situated in zones with a warm climate. Thus, reflective coatings have emerged as an alternative to reject a fraction of the solar energy received by roofs. In this research, the thermal behavior of concrete slab roofs with traditional and solar reflective coatings was simulated using a computational tool. The studied slab configurations belong to two groups, non-insulated and insulated roofs. In the second group, the thermal insulation thickness complies with the value recommended by a national building energy standard. Weather data from four cities in Mexico with a warm climate were used as boundary conditions for the exterior surface of the roofs. The computational tool consisted of a numerical model based on the finite volume method, which was validated with experimental data. A series of comparative simulations was developed, taking a gray roof as the control case. The results demonstrated that white roofs without insulation had an exterior surface temperature between 11 and 16 °C lower than the gray roof without insulation. Thus, the daily heat gain of these white roofs was reduced by a factor ranging between 41 and 54%. On the other hand, white roofs with insulation reduced the exterior surface temperature between 17 and 21 °C compared to the gray roof with insulation. This temperature reduction caused insulated white roofs to have a daily heat gain between 37 and 56% smaller than the control case. Another contribution of this research is the assessment of two retrofitting techniques when they are applied at once. In other words, a comparison between a non-insulated gray roof and an insulated white roof revealed that the latter roof had a daily heat gain up to 6.4-times smaller than the first.
The Deltah Lab, a New Multidisciplinary European Facility to Support the H2 Distribution & Storage Economy
Authors: Stelitano Sara , Rullo Alberto , Piredda Luigi , Mecozzi Elisabetta , Di Vito Luigi , Agostino Raffaele Giuseppe , Filosa Raffaele , Formoso Vincenzo , Conte Giuseppe , Policicchio Alfonso
Published: April 2021

The target for European decarburization encourages the use of renewable energy sources and H2 is considered the link in the global energy system transformation. So, research studies are numerous, but only few facilities can test materials and components for H2 storage. This work offers a brief review of H2 storage methods and presents the preliminary results obtained in a new facility. Slow strain rate and fatigue life tests were performed in H2 at 80 MPa on specimens and a tank of AISI 4145, respectively. Besides, the storage capacity at 30 MPa of a solid-state system, they were evaluated on kg scale by adsorption test. The results have shown the H2 influence on mechanical properties of the steel. The adsorption test showed a gain of 26% at 12 MPa in H2 storage with respect to the empty condition. All samples have been characterized by complementary techniques in order to connect the H2 effect with material properties.
Finite Element Analysis on Block Shear Mechanism of Lean Duplex Stainless Steel Welded Connections
Authors: Cho YongHyun , Kim Dong-Keon , Kim JunSu , Kim TaeSoo
Published: April 2021

The block shear equations specified in the current AISC specification for structural steel buildings and North American cold-formed steel design specifications are based on research results of carbon steel bolted connections. These equations were found to be inapplicable for the welded connections in the literature. This issue is primarily associated with the use of the incorrect assumption on block shear failure mechanism. The present paper examines the accuracy of various block shear equations available in the design specifications and in the literature. The paper also examines the shear hardening capacity and the level of tensile stress over the critical net area with the results of finite element analysis, in which the fracture simulation is considered. It shows that the block shear capacities of lean duplex stainless steel welded connections can be predicted accurately using tensile stress equal to 1.25Fu, as proposed in the literature.
Selective Use of Corn Crop Residues: Energy Viability
Authors: Miranda M. T. , García-Mateos R. , Arranz J. I. , Sepúlveda F. J. , Romero P. , Botet-Jiménez A.
Published: April 2021

The corn crop is one of the most important crops worldwide. However, the management of the residues generated is not efficient enough, which diminishes the competitiveness of this crop. An interesting option for the valorization of these wastes is their thermal use. In order to make the management of this residue as much efficient as possible, it is vital to know the different processes related to a corn harvest, and try to adapt the use of this waste depending on its characteristics. Thus, in this work, and on the basis of a conventional corn harvest, a differentiated analysis of the residue generated was carried out, including its characterization and assessing its behavior during pyrolysis and combustion. The results pointed out the importance of collecting residue immediately after its generation and avoiding its contact with the soil as this factor tends to worsen its thermal properties and ash content. Concerning the selective collection, it is highly advisable if the subsequent thermal use is going to be a pyrolytic process. In the case of combustion, even though the samples that contain corn stalk showed better combustion properties, this improvement did not outweigh the adverse effects related to the increase in ash content, especially for its pelletizing.
A Deep Multi-Frame Super-Resolution Network for Dynamic Scenes
Authors: Pan Ze , Tan Zheng , Lv Qunbo
Published: April 2021

The multi-frame super-resolution techniques have been prosperous over the past two decades. However, little attention has been paid to the combination of deep learning and multi-frame super-resolution. One reason is that most deep learning-based super-resolution methods cannot handle variant numbers of input frames. Another reason is that it is hard to capture accurate temporal and spatial information because of the misalignment of input images. To solve these problems, we propose an optical-flow-based multi-frame super-resolution framework, which is capable of dealing with various numbers of input frames. This framework enables to make full use of the input frames, allowing it to obtain better performance. In addition, we use a spatial subpixel alignment module for more accurate subpixel-wise spatial alignment and introduce a dual weighting module to generate weights for temporal fusion. Both two modules lead to more effective and accurate temporal fusion. We compare our method with other state-of-the-art methods and conduct ablation studies on our method. The results of qualitative and quantitative analyses show that our method achieves state-of-the-art performances, demonstrating the advantage of the designed framework and the necessity of proposed modules.
Biology of Drug-Induced Gingival Hyperplasia: In Vitro Study of the Effect of Nifedipine on Human Fibroblasts
Authors: Lauritano Dorina , Moreo Giulia , Vella Fedora Della , Palmieri Annalisa , Carinci Francesco , Petruzzi Massimo
Published: April 2021

Background: It has been proven that the antihypertensive agent nifedipine can cause gingival overgrowth as a side effect. The aim of this study was to analyze the effects of pharmacological treatment with nifedipine on human gingival fibroblasts activity, investigating the possible pathogenetic mechanisms that lead to the onset of gingival enlargement. Methods: The expression profile of 57 genes belonging to the “Extracellular Matrix and Adhesion Molecules” pathway, fibroblasts’ viability at different drug concentrations, and E-cadherin levels in treated fibroblasts were assessed using real-time Polymerase Chain Reaction, PrestoBlue™ cell viability test, and an enzyme-linked immunoassay (ELISA), respectively. Results: Metalloproteinase 24 and 8 (MMP24, MMP8) showed significant upregulation in treated cells with respect to the control group, and cell adhesion gene CDH1 (E-cadherin) levels were recorded as increased in treated fibroblasts using both real-time PCR and ELISA. Downregulation was observed for transmembrane receptors ITGA6 and ITGB4, the basement membrane constituent LAMA1 and LAMB1, and the extracellular matrix protease MMP11, MMP16, and MMP26. Conclusions: The obtained data suggested that the pathogenesis of nifedipine-induced gingival overgrowth is characterized by an excessive accumulation of collagen due to the inhibition of collagen intracellular and extracellular degradation pathways.
ATP7A-Regulated Enzyme Metalation and Trafficking in the Menkes Disease Puzzle
Authors: Horn Nina , Wittung-Stafshede Pernilla
Published: April 2021

Copper is vital for numerous cellular functions affecting all tissues and organ systems in the body. The copper pump, ATP7A is critical for whole-body, cellular, and subcellular copper homeostasis, and dysfunction due to genetic defects results in Menkes disease. ATP7A dysfunction leads to copper deficiency in nervous tissue, liver, and blood but accumulation in other tissues. Site-specific cellular deficiencies of copper lead to loss of function of copper-dependent enzymes in all tissues, and the range of Menkes disease pathologies observed can now be explained in full by lack of specific copper enzymes. New pathways involving copper activated lysosomal and steroid sulfatases link patient symptoms usually related to other inborn errors of metabolism to Menkes disease. Additionally, new roles for lysyl oxidase in activation of molecules necessary for the innate immune system, and novel adapter molecules that play roles in ERGIC trafficking of brain receptors and other proteins, are emerging. We here summarize the current knowledge of the roles of copper enzyme function in Menkes disease, with a focus on ATP7A-mediated enzyme metalation in the secretory pathway. By establishing mechanistic relationships between copper-dependent cellular processes and Menkes disease symptoms in patients will not only increase understanding of copper biology but will also allow for the identification of an expanding range of copper-dependent enzymes and pathways. This will raise awareness of rare patient symptoms, and thus aid in early diagnosis of Menkes disease patients.
The Tumour Vasculature as a Target to Modulate Leucocyte Trafficking
Authors: Zhao Yang , Ting Ka Ka , Coleman Paul , Qi Yanfei , Chen Jinbiao , Vadas Mathew , Gamble Jennifer
Published: April 2021

The effectiveness of immunotherapy against solid tumours is dependent on the appropriate leucocyte subsets trafficking and accumulating in the tumour microenvironment (TME) with recruitment occurring at the endothelium. Such recruitment involves interactions between the leucocytes and the endothelial cells (ECs) of the vessel and occurs through a series of steps including leucocyte capture, their rolling, adhesion, and intraluminal crawling, and finally leucocyte transendothelial migration across the endothelium. The tumour vasculature can curb the trafficking of leucocytes through influencing each step of the leucocyte recruitment process, ultimately producing an immunoresistant microenvironment. Modulation of the tumour vasculature by strategies such as vascular normalisation have proven to be efficient in facilitating leucocyte trafficking into tumours and enhancing immunotherapy. In this review, we discuss the underlying mechanisms of abnormal tumour vasculature and its impact on leucocyte trafficking, and potential strategies for overcoming the tumour vascular abnormalities to boost immunotherapy via increasing leucocyte recruitment.
Role for the Histone Demethylase KDM4B in Rhabdomyosarcoma via CDK6 and CCNA2: Compensation by KDM4A and Apoptotic Response of Targeting Both KDM4B and KDM4A
Authors: Walters Zoë S. , Aladowicz Ewa , Villarejo-Balcells Barbara , Nugent Gary , Selfe Joanna L. , Eve Paul , Blagg Julian , Rossanese Olivia , Shipley Janet
Published: April 2021

Histone demethylases are epigenetic modulators that play key roles in regulating gene expression related to many critical cellular functions and are emerging as promising therapeutic targets in a number of tumor types. We previously identified histone demethylase family members as overexpressed in the pediatric sarcoma, rhabdomyosarcoma. Here we show high sensitivity of rhabdomyosarcoma cells to a pan-histone demethylase inhibitor, JIB-04 and identify a key role for the histone demethylase KDM4B in rhabdomyosarcoma cell growth through an RNAi-screening approach. Decreasing KDM4B levels affected cell cycle progression and transcription of G1/S and G2/M checkpoint genes including CDK6 and CCNA2, which are bound by KDM4B in their promoter regions. However, after sustained knockdown of KDM4B, rhabdomyosarcoma cell growth recovered. We show that this can be attributed to acquired molecular compensation via recruitment of KDM4A to the promoter regions of CDK6 and CCNA2 that are otherwise bound by KDM4B. Furthermore, upfront silencing of both KDM4B and KDM4A led to RMS cell apoptosis, not seen by reducing either alone. To circumvent compensation and elicit stronger therapeutic responses, our study supports targeting histone demethylase sub-family proteins through selective poly-pharmacology as a therapeutic approach.
Epithelial and Mesenchymal Markers in Adrenocortical Tissues: How Mesenchymal Are Adrenocortical Tissues?
Authors: Sbiera Iuliu , Kircher Stefan , Altieri Barbara , Fassnacht Martin , Kroiss Matthias , Sbiera Silviu
Published: April 2021

A clinically relevant proportion of adrenocortical carcinoma (ACC) cases shows a tendency to metastatic spread. The objective was to determine whether the epithelial to mesenchymal transition (EMT), a mechanism associated with metastasizing in several epithelial cancers, might play a crucial role in ACC. 138 ACC, 29 adrenocortical adenomas (ACA), three normal adrenal glands (NAG), and control tissue samples were assessed for the expression of epithelial (E-cadherin and EpCAM) and mesenchymal (N-cadherin, SLUG and SNAIL) markers by immunohistochemistry. Using real-time RT-PCR we quantified the alternative isoform splicing of FGFR 2 and 3, another known indicator of EMT. We also assessed the impact of these markers on clinical outcome. Results show that both normal and neoplastic adrenocortical tissues lacked expression of epithelial markers but strongly expressed mesenchymal markers N-cadherin and SLUG. FGFR isoform splicing confirmed higher similarity of adrenocortical tissues to mesenchymal compared to epithelial tissues. In ACC, higher SLUG expression was associated with clinical markers indicating aggressiveness, while N-cadherin expression inversely associated with these markers. In conclusion, we could not find any indication of EMT as all adrenocortical tissues lacked expression of epithelial markers and exhibited closer similarity to mesenchymal tissues. However, while N-cadherin might play a positive role in tissue structure upkeep, SLUG seems to be associated with a more aggressive phenotype.
Circulating Tumor DNA Reflects Uveal Melanoma Responses to Protein Kinase C Inhibition
Authors: Park John J. , Diefenbach Russell J. , Byrne Natalie , Long Georgina V. , Scolyer Richard A. , Gray Elin S. , Carlino Matteo S. , Rizos Helen
Published: April 2021

The prognosis for patients with UM is poor, and recent clinical trials have failed to prolong overall survival (OS) of these patients. Over 95% of UM harbor activating driver mutations, and this allows for the investigation of ctDNA. In this study, we investigated the value of ctDNA for adaptive clinical trial design in metastatic UM. Longitudinal plasma samples were analyzed for ctDNA in 17 metastatic UM patients treated with PKCi-based therapy in a phase 1 clinical trial setting. Plasma ctDNA was assessed using digital droplet PCR (ddPCR) and a custom melanoma gene panel for targeted next generation sequencing (NGS). Baseline ctDNA strongly correlated with baseline lactate dehydrogenase (LDH) (p < 0.001) and baseline disease burden (p = 0.002). Early during treatment (EDT) ctDNA accurately predicted patients with clinical benefit to PKCi using receiver operator characteristic (ROC) curves (AUC 0.84, [95% confidence interval 0.65–1.0, p = 0.026]). Longitudinal ctDNA assessment was informative for establishing clinical benefit and detecting disease progression with 7/8 (88%) of patients showing a rise in ctDNA and targeted NGS of ctDNA revealed putative resistance mechanisms prior to radiological progression. The inclusion of longitudinal ctDNA monitoring in metastatic UM can advance adaptive clinical trial design.
Clinical Validation of the Comprehensive Complication Index in Colon Cancer Surgery
Authors: Tamini Nicolò , Bernasconi Davide , Ripamonti Lorenzo , Lo Bianco Giulia , Braga Marco , Nespoli Luca
Published: April 2021

(1) Introduction: To date, the sensitivity of the Comprehensive Complication Index (CCI) in a homogeneous cohort of colonic resections for oncologic purposes has not been reported. The present study aims to compare the CCI with the conventional Clavien–Dindo classification (CDC) in colon cancer patients. (2) Methods: The clinical data of patients submitted to an elective colectomy for adenocarcinoma were retrieved from a prospectively maintained database. Postoperative complications and length of stay were reviewed, and CDC and CCI scores were calculated for each patient. The association of the CCI and the CDC with the length of stay, prolongation of stay and readmission rate were assessed and compared. (3) Results: The overall postoperative morbidity was 26.9%. In particular, 157 (20.4%) patients had more than one complication. A strong correlation between the two scoring systems was observed (r = 99.4%; 95%CI: 99.3–99.5%). In multivariate analysis, CCI had a higher predictive ability for all endpoints. Regarding subgroup analysis, the difference between the CCI and CDC was progressively increased when evaluating outcome measures in complicated and multi-complicated patients. (4) Conclusion: Both scoring systems adequately report the overall burden of postoperative complications. The CCI showed a greater ability than the CDC to predict hospital stay, particularly in patients with multiple postoperative complications.
Sirtuin 1 and Skin: Implications in Intrinsic and Extrinsic Aging—A Systematic Review
Authors: Bielach-Bazyluk Angelika , Zbroch Edyta , Mysliwiec Hanna , Rydzewska-Rosolowska Alicja , Kakareko Katarzyna , Flisiak Iwona , Hryszko Tomasz
Published: April 2021

Skin, as the outermost organ of the body, is constantly exposed to both intrinsic and extrinsic causative factors of aging. Intrinsic aging is related to compromised cellular proliferative capacity, and may be accelerated by harmful environmental influences with the greatest significance of ultraviolet radiation exposure, contributing not only to premature aging, but also to skin carcinogenesis. The overall skin cancer burden and steadily increasing global antiaging market provide an incentive for searching novel targets to improve skin resistance against external injury. Sirtuin 1, initially linked to extension of yeast and rodent lifespan, plays a key role in epigenetic modification of proteins, histones, and chromatin by which regulates the expression of genes implicated in the oxidative stress response and apoptosis. The spectrum of cellular pathways regulated by sirtuin 1 suggests its beneficial impact on skin aging. However, the data on its role in carcinogenesis remains controversial. The aim of this review was to discuss the relevance of sirtuin 1 in skin aging, in the context of intrinsic factors, related to genetic premature aging syndromes, as well as extrinsic modifiable ones, with the assessment of its future application. PubMed were searched from inception to 4 January 2021 for relevant papers with further search carried out on ClinicalTrials.gov. The systematic review included 46 eligible original articles. The evidence from numerous studies proves sirtuin 1 significance in both chronological and premature aging as well as its dual role in cancer development. Several botanical compounds hold the potential to improve skin aging symptoms.
Molecular Mechanisms of Maternal Diabetes Effects on Fetal and Neonatal Surfactant
Authors: Yildiz Atar Hilal , Baatz John E. , Ryan Rita M.
Published: April 2021

Respiratory distress is a significant contributor to newborn morbidity and mortality. An association between infants of diabetic mothers (IDMs) and respiratory distress syndrome (RDS) has been well recognized for decades. As obesity and diabetes prevalence have increased over the past several decades, more women are overweight and diabetic in the first trimester, and many more pregnant women are diagnosed with gestational diabetes. Glycemic control during pregnancy can be challenging due to the maternal need for higher caloric intake and higher insulin resistance. Surfactant is a complex molecule at the alveolar air–liquid interface that reduces surface tension. Impaired surfactant synthesis is the primary etiology of RDS. In vitro cell line studies, in vivo animal studies with diabetic rat offspring, and clinical studies suggest hyperglycemia and hyperinsulinemia can disrupt surfactant lipid and protein synthesis, causing delayed maturation in surfactant in IDMs. A better understanding of the molecular mechanisms responsible for surfactant dysfunction in IDMs may improve clinical strategies to prevent diabetes-related complications and improve neonatal outcomes.
Euglycemic Diabetic Ketoacidosis after a Single Dose of Empagliflozin in a Patient with Pancreatitis
Authors: Calçada Marta Brandão , Fernandes Luís , Soares Costa Rita , Montezinho Sara , Martins Duarte Filipa , Frutuoso Luísa , Freitas Ana Raquel
Published: April 2021

Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are the most recently approved drug class for the treatment of type 2 diabetes mellitus (T2D). Although they are largely well-tolerated, their intake has been associated with euglycemic diabetic ketoacidosis (DKA) in some rare cases. We report the case of a 70-year-old male with type 2 diabetes and no history of DKA, who started therapy with empagliflozin one day before presenting with acute pancreatitis and laboratory findings consistent with euglycemic DKA. SGLT2i can induce euglycemic DKA from the first dose. Given the atypical presentation, a high degree of clinical suspicion is required to recognize this complication.