bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and characterization of a novel type-2 DGAT homologue gene from the green microalga Haematococcus pluvialis
Authors: Zhang Hongjiang , Hang Wei , Zhao Chunchao , Xu Wenxin , Zhu Xiaoli , Ji Chunli , Xue Jinai , Zhang Chunhui , Li Runzhi , Cui Hongli
Published: February 2020

Background: The unicellular green microalga Haematococcus pluvialis is an ideal source of astaxanthin (AST) which is stored at oil bodies contain both esterified astaxanthin (EAST) and triacylglycerol (TAG). Diacylglycerol acyltransferase (DGAT) catalyzes the last step of the acyl-CoA-dependent TAG biosynthesis and is considered as the crucial enzyme involving in EAST biosynthesis in H. pluvialis. However, the function of DGAT in H. pluvialis has not been reported.
Results: A full-length cDNA sequences encoding a putative DGAT2 (HaeDGAT2E) was obtained from H. pluvialis . It contained an open reading frame (ORF) of 1,017-bp encoding a protein of 338 amino acid residues. The isolated HaeDGAT2E protein shared high identity of 57.6% and 54.1% with DGAT2E from Chlamydomonas reinhardtii and Chromochloris zofingiensis respectively. There were 7 conserved motifs and 3 trans-membrane regions in HaeDGAT2E. The phylogenetic analysis suggested that HaeDGAT2E belonged to DGAT2E subfamily. HaeDGAT2E activity was confirmed in the TAG deficient yeast strain (H1246) by restoring its ability to produce TAG. Upon expression of HaeDGAT2E , C16:0 and C18:1 fatty acid contents were 190.2% and 132.4% higher respectively than that of the H1246 strain. In addition, over-expression of HaeDGAT2E in transgenic Nicotiana Benthamiana resulted in increased contents of C16:0 (113.5%) and C18:1 (234.5%).
Conclusions: A novel gene encoding HaeDGAT2E was identified in H. pluvialis . This is the first functional analysis of DGAT2 in Haematococcus . This information is important for understanding TAG accumulation and for further elucidating EAST biosynthesis in H. pluvialis .
Evaluation of Housekeeping Gene Expression Stability in Carnation (Dianthus caryophyllus)
Authors: Luo Huolin , Yu Wenjing , Tao Yuan , Hrovat Jonathan , Xue Ahui , Luo Liping
Published: February 2020

Background: The real-time quantitative reverse transcription PCR (RT-qPCR) is widely used for gene expression analysis, owing to its advantages of high specificity, sensitivity and repeatability. A suitable reference gene is an absolute prerequisite for accurate normalization, nevertheless, the frequently-used reference gene was reported unstable under different experimental conditions and causes failure to correctly analyze the expression of the interested gene. Therefore, it is vital to systematically evaluate the expression stability of these candidate reference genes before performing RT-qPCR. Results: In this study, two computational statistical methods were used, including geNorm and NormFinder, in order to determine the expression stability of 12 frequently-used reference genes in Dianthus caryophyllus across different experimental conditions. The results show that the expression stability of candidate genes varies greatly in different sample pools, which again proves the instability of these common housekeeping gene expressions. In general, the expression of UBQ10 (ubiquitin10), EF1a (elongation factor 1A) and TIP41 (TIP41-like family protein) were relatively stable under different experimental conditions, while the expression stability of 18S (18S Ribosome RNA), TIF5A (translation initiation factor 5A) and PP2A (protein phosphatase 2A) were relatively poor. Conclusion: EF1α, TIP41 and UBQ10 were considered the most appropriate reference genes when all samples were put together, while UBQ10 was most stable in exogenous hormone treatments. TUB and UBQ10 can be used as reliable internal control genes under stress, while CYP and TUA can act as reliable internal controls in different tissues. This is the first systematic study of selection of reference genes in Carnation, and will benefit future expression studies in this crop.
Implementation of a patient centred medical home (PCMH) initiative in general practices in New South Wales, Australia
Authors: Metusela Christine , Dijkmans-Hadley Bridget , Mullan Judy , Gow Andrew , Bonney Andrew
Published: February 2020

Background With an ageing population and an increase in chronic disease burden in Australia, Patient Centred Medical Home (PCMH) models of care have been identified as potential options for primary care reform and improving health care outcomes. Adoption of PCMH models are not well described outside of North America. We examined the experiences of seven general practices in an Australian setting that implemented projects aligned with PCMH values and goals supported by their local Primary Health Network (PHN). Method Qualitative and quantitative data were collected over a twelve month period, including semi-structured interviews, participant observation, and practice data to present a detailed examination of a subject of study; the implementation of PCMH projects in seven general practices. We conducted 49 interviews with general practitioners, practice managers, practice nurses and PHN staff. Framework analysis deploying the domains of a logic model was used to synthesis and analyse the data. Findings Facilitators in implementing successful, sustainable change included the capacity and willingness of practices to undertake change; whole of practice engagement with a shared vision towards PCMH change; engaged leadership; training and support; and structures and processes required to provide team-based, data driven care. Barriers to implementation included change fatigue, challenges of continued engaged leadership and insufficient time to implement PCMH change. Conclusion Our study examined the experiences of implementing PCMH initiatives in Australian general practice setting, describing facilitators and barriers to PCMH change. Our findings provide guidance for PHNs and practices within Australia, as well as general practice settings internationally, that are interested in undertaking similar quality improvement projects.
MEX3A promotes the proliferation, migration, and invasion of non-small-cell lung cancer by activating the MEK/ERK pathway.
Authors: Zhang Yao , Ren Hongjiu , Zhang Bo , Xu Yitong , Zhang Qingfu , Jiang Jun , Wudu Muli , Su Hongbo , Jiang Xizi , Liu Zongang , Zou Zifang , Qiu Xueshan
Published: February 2020

Background: Lung cancer is the second most prevalent type of cancer worldwide; however, its death is the highest among all cancers. Previous studies have shown that MEX3A is associated with Argonaute (Ago) and CDX2 proteins, which are important in tumor progression. High expression of MEX3A is also known to negatively affect the therapeutic impact of radiotherapy and chemotherapy. Therefore, we investigated the role of MEX3A in non-small cell lung cancer (NSCLC).Methods: Tumor specimens were collected from 87 NSCLC patients who underwent surgical resection between 2014 and 2019 at the Pathology Archive of the First Affiliated Hospital of China Medical University, and were subsequently used for immunohistochemistry. NSCLC cell lines and normal bronchial epithelial HBE cell line were used to study the effects of MEX3A by utilizing MEX3A-siRNA, MEX3A-plasmids, and the ERK pathway inhibitor U0126. Western blotting, colony formation assays, MTT assays, and Transwell assays with or without Matrigel were performed to explore the impact of MEX3A on NSCLC.Results: MEX3A protein expression was associated with tumor differentiation (P=0.044), size (P=0.020), lympth node status (P=0.001), and p-TNM stage (P=0.009) and was preferentially expressed in tumor tissues. MEX3A was found to be localized to the nucleus. The expression level in cancer tissue was significantly higher than that in normal tissue. According to our study, MEX3A was shown to promote the proliferation, migration, and invasion of NSCLC. In addition, MEX3A regulated the expression of P-MEK, P-ERK, CyclinA2, CDK4, CDK6, RhoA and MMP2. The MEK inhibitor U0126 reduced the biological effects of MEX3A , suggesting that MEX3A increases the carcinogenic activity of NSCLC cells through activation of the MEK/ERK signaling pathway.Conclusion: These results suggest an association between MEX3A expression and the unfavorable clinicopathological characteristics of NSCLC patients. And MEX3A promote NSCLC progression by increasing the proliferative and metastatic potential of cancer cells through upregulation of the MEK/ERK pathway. We propose that MEX3A is a candidate prognostic biomarker and potential therapeutic target in NSCLC.
Moderate low temperature protects the ischemic brain injury by activating SIRT1 through inhibition of FOXO1/PINK1/Parkin axis mediated mitophagy in a mouse
Authors: Zhu Yaobin , Zhang Yaping , Ding Nan , Yi Hanlu , Fan Xing , Li Zhiqiang
Published: February 2020

Background: Several evidences suggested that the protective effect of hypothermia on brain injury is related to the inhibition of apoptosis and depends on the onset time of hypothermia and the degree of brain maturation. We performed many experiments aimed to comprehensively explore the biological functions of moderate low temperature protects the ischemic brain injury in a mouse and its underlying mechanism.Methods: 12 normal healthy C57BL6 mouse were selected, and moderate low temperature model mouse were selected. The biological functions of moderate low temperature protect the ischemic brain injury in a mouse and its underlying mechanism were performed to explore.Results: Based on our results, we found that moderate hypothermia brain protection could alleviate cerebral injury caused by ischemia reperfusion in mouse. Hypothermic brain protection reduced the level of oxidative stress induced by ischemia reperfusion in mouse. Meso-hypothermic cerebral protection could inhibit excessive mitochondrial autophagy induced by ischemia reperfusion in mouse. Medium-low temperature brain protection could activate SIRT1 and inhibit FOXO1/PINK1/Parkin pathway. Activation of SIRT1 in the hypoxia/reoxygenation model of hippocampal neurons could inhibit autophagy and oxidative stress by inhibiting the FOXO1/PINK1/Parkin pathway.Conclusions : Moderate low temperature protects the ischemic brain injury by activating SIRT1 through inhibition of FOXO1/PINK1/Parkin axis mediated mitophagy in a mouse.
The charged $Z_c$ and $Z_b$ structures in a constituent quark model approach
Authors: G. Ortega Pablo , Segovia Jorge , Rodriguez Entem David , Fernández Francisco
Published: February 2020

The nature of the recently discovered Z_cZc
and Z_bZb
structures is intriguing. Their charge forces its minimal quark content
to be Q\bar Q q\bar qQQ‾qq‾
(where Q=\{c,b\}Q={c,b}
and q=\{u,d\}q={u,d}).
In this work we perform a molecular coupled-channels calculation of the
I^G(J^{PC})=1^+(1^{+-})IG(JPC)=1+(1+−)
charm and bottom sectors in the framework of a constituent quark model
which satisfactorily describes a wide range of properties of
(non-)conventional hadrons containing heavy quarks. All the relevant
channels are included for each sector, i.e.: The
D^{(\ast)}\bar D^{\ast}+h.c.D(*)D‾*+h.c.,
\pi J/\psiπJ/ψ
and \rho\eta_cρηc
channels for the Z_cZc
and B^{(\ast)}B^{\ast}B(*)B*
and \Upsilon(nS)\piΥ(nS)π
(n=1,2,3n=1,2,3)
channels for the Z_bZb
analysis. Possible structures of these resonances will be discussed.
Generalized parton distribution functions of $\rho$ meson
Authors: Sun Baodong , Dong Yubing
Published: February 2020

We report our recent studies of generalized parton distribution
functions of a \rhoρ
meson with the help of a light-front constituent quark model. The
electromagnetic form factors and structure functions of the system are
discussed. Moreover, we show our results for its gravitational form
factors (or energy-momentum tensor form factors) and other mechanical
properties, like mass distributions, pressures, shear forces, and
D-D−term.
The eikonal model of reactions involving exotic nuclei; Roy Glauber's legacy in today's nuclear physics
Authors: Capel Pierre
Published: February 2020

In this contribution, the eikonal approximation developed by Roy
Glauber to describe high-energy quantum collisions is presented. This
approximation has been—and still is—extensively used to analyse reaction
measurements performed to study the structure of nuclei far from
stability. This presentation focuses more particularly on the
application of the eikonal approximation to the study of halo nuclei in
modern nuclear physics. To emphasise Roy Glauber’s legacy in today’s
nuclear physics, recent extensions of this model are reviewed.
Dipolar condensed atomic mixtures and miscibility under rotation
Authors: Tomio Lauro , Kumar Ramavarmaraja Kishor , Gammal Arnaldo
Published: February 2020

By considering symmetric- and asymmetric-dipolar coupled mixtures
(with dysprosium and erbium isotopes), we report a study on relevant
anisotropic effects, related to spatial separation and miscibility, due
to dipole-dipole interactions (DDIs) in rotating binary dipolar
Bose-Einstein condensates. The binary mixtures are kept in strong
pancake-like traps, with repulsive two-body interactions modeled by an
effective two-dimensional (2D) coupled Gross-Pitaevskii equation. The
DDI are tuned from repulsive to attractive by varying the dipole
polarization angle. A clear spatial separation is verified in the
densities for attractive DDIs, being angular for symmetric mixtures and
radial for asymmetric ones. Also relevant is the mass-imbalance
sensibility observed by the vortex-patterns in symmetric- and
asymmetric-dipolar mixtures. In an extension of this study, here we show
how the rotational properties and spatial separation of these dipolar
mixture are affected by a quartic term added to the harmonic trap of one
of the components.
Fluctuations of work in realistic equilibrium states of quantum systems with conserved quantities
Authors: Mur-Petit Jordi , Relaño Armando , Molina Rafael A. , Jaksch Dieter
Published: February 2020

The out-of-equilibrium dynamics of quantum systems is one of the most
fascinating problems in physics, with outstanding open questions on
issues such as relaxation to equilibrium. An area of particular interest
concerns few-body systems, where quantum and thermal fluctuations are
expected to be especially relevant. In this contribution, we present
numerical results demonstrating the impact of conserved quantities (or
‘charges’) in the outcomes of out-of-equilibrium measurements starting
from realistic equilibrium states on a few-body system implementing the
Dicke model.
Study of the hyperon-nucleon interaction via final-state interactions in exclusive reactions
Authors: Zachariou Nicholas , Watts Daniel , Ilieva Yordanka
Published: February 2020

A novel approach that allows access to long-sought information on the
Hyperon-Nucleon (YN) interaction was developed by producing a hyperon
beam within a few-body nuclear system, and studying final-state
interactions. The determination of polarisation observables, and
specifically the beam spin asymmetry, in exclusive reactions allows a
detailed study of the various final-state interactions and provides us
with the tools needed to isolate kinematic regimes where the YN
interaction dominates. High-statistics data collected using the CLAS
detector housed in Hall-B of the Thomas Jefferson laboratory allows us
to obtain a large set of polarisation observables and place stringent
constraints on the underlying dynamics of the YN interaction.
Muonic Lithium atoms: nuclear structure corrections to the Lamb shift
Authors: Li Muli Simone Salvatore , Poggialini Anna , Bacca Sonia
Published: February 2020

In view of the future plans to measure the Lamb shift in muonic
Lithium atoms we address the microscopic theory of the
\mu\,μ-^66Li^{2+}2+
and \mu\,μ-^77Li^{2+}2+
systems. The goal of the CREMA collaboration is to measure the Lamb
shift to extract the charge radius with high precision and compare it to
electron scattering data or atomic spectroscopy to see if interesting
puzzles, such as the proton and deuteron radius puzzles, arise. For this
experiment to be successful, theoretical information on the nuclear
structure corrections to the Lamb shift is needed. For
\muμ-^66Li^{2+}2+
and \mu\,μ-^77Li^{2+}2+
there exist only estimates of nuclear structure corrections based on
experimental data that suffer from very large uncertainties. We present
the first steps towards an ab initio computation of these quantities
using few-body techniques.
A Summarized Review on Friction Stir Welding for Aluminum Alloys
Authors: Gadallah Nabil , Sabry Ibrahim , Ghafaar M.Abdel
Published: February 2020

This paper explains the precept and methodology of FSW. It covers some of the technical sides which influence the process and quality of FSW joint. Large advance has been accomplished in friction stir welding (FSW) of aluminum in every side of tool manufacture, microstructure properties estimate in the last decennia. With the development of reliable welding tools and precise control systems, FSW of aluminum has reached a new level of technical maturity. influence on butt joint arrangement is studied. Effect on welding quality of main parameters:  rotation speed, travel speed, tool tilt angle, axial force and weld time has been studied. Finally, FSW is identified as an additional area for research can be carried out in the welding science.
Creativity Management: An Approach For Achieving Efficient Workplace Environment
Authors: Ain Omar Mohamed , Khodeir Laila Mohamed
Published: February 2020

Workplace environment is defined as the current conditions of the surroundings of a workplace. More than 80% of performance problems are influenced by the workplace environment where efficiency of workplace environment represents the total amount of work done by one employee in a single day. There are different types and traits of employees in Architectural Design firms , where only 5 types are being analyzed in this research paper which encounters; creative, innovative ,Talented , Technical & Analytical Architects that should be considered to achieve an efficient workplace environment. Hence , The Problem of this paper the inefficient management creating inefficient management for architects results in the dissatisfaction of employees while boosting the rate of turnovers impacting the productivity negatively. One approach that has been introduced to tackle such problem is creativity management which could embrace the 5 types of employees in Architectural design firms. Thus, the main purpose of this study is introducing how creativity management approach can effect the workplace environment and increase its efficiency  in Architectural design firms . To achieve the aim of this paper , a comprehensive literature review analysis has been done on recently published Research papers from 2000 to 2018. This includes discussing the contribution of authors on the approaches for efficient workplace environment , and introducing creativity management approach in workplace environment in Architectural Design firms. Hence, Findings of this paper took the form of impact analysis , where the effect of creativity management approach on achieving efficient workplace has been identified.
Health and Safety Considerations in Mega Construction Projects
Authors: Waheed Waad , Khodeir Laila Mohamed
Published: February 2020

The construction industry is considered one of the most hazardous industries especially in Mega projects, not only in developing countries but also throughout the world due to its unique nature. The hazards of this industry make it very essential to pay more attention to construction safety and to improve the safety performance of construction companies. However, safety is not a luxury but a necessity, and may be considered an important function to be used against unnecessary loss. International Mega Construction projects are likely to become increasingly common in the engineering and construction industry. A Mega-project as a high impact technically complex project which requires careful advanced planning, large projects are defined as those with construction work valued in excess of US $1billion. On such projects, there may be 60 large contracting organizations and 30 to 40 consulting companies. The construction workforce could total 45,000 personnel delivering over 10 million man hours of effort per month at peak periods, so there is a need of guidance material that can be used to improve the management of health and safety(H&S) throughout these type of projects. Thus the aim of this paper is to identify the H&S considerations that are related to Mega Construction Projects. To achieve the aim of this paper a literature review analysis has been performed where more than hundred research paper that have been published from 1998, to 2018 have been analyzed. Findings of this paper have managed to identify the nature of mega construction projects, the root causes of accidents and the consequence of poor health and safety management in mega construction projects. This paper is considered of value to construction project officers and Project managers working in mega construction projects in Egypt and MENA region.
Sustainable Architectural Design Education: An Improved Experimental Method in a 3rd Year Design Studio
Authors: Mohamed Kamal Eldin
Published: February 2020

The design studio is a unique class format within the architectural curriculum education, in which learning is based on student-instructor interaction and learning by experiences while architectural design is the collective of knowledge and skills to accomplish a unique expectation of a product. Sustainable design has been incorporated into many architecture curriculum education programs but there has been lack of merging in design studio project. This study introduces an improved method of the first experimental Sustainable Architectural Design Studio (SADS) with restructured teaching tools for integrating sustainability principles in design studio along with the studio outcome. In additional, it presents the evaluation and the assessment of the improved method as well as the finding along with the recommendations for the future experimental.
Photodegradation of microcystin-LR by pyridyl iron porphyrin immobilized on NaY zeolite
Authors: Wang Shulian , Zhang Huiqin , Ge Hongmei , Shi Yafei , Li Zhu
Published: February 2020

A novel photocatalyst, FeTPyPY, was prepared by immobilizing water-soluble tetra(4-pyridyl)phenyl iron-porphyrin (FeTPyP) on NaY zeolite to degrade microcystin-LR (MC-LR), one of the most toxic microcystins (MCs). UV-Vis analysis, UV-Vis diffuse reflectance spectroscopy, infrared spectroscopy, cyclic voltammetry and transmission electron microscopy were employed to characterize immobilized FeTPyPY. Under visible light (λ ≥ 420 nm), MC-LR was degraded utilizing immobilized FeTPyPY by activating molecular oxygen. The results showed that 85% of MC-LR was efficiently degraded by FeTPyPY with loading amount 100:1 (mNaY:mFeTPyP) after 300 min of visible light illumination. Moreover, FeTPyPY was stable in the degradation system with pH 7.0. The degradation mechanism was evaluated using electron spin resonance, and the results demonstrated that highly reactive oxygen species (•OH radical) were generated in the system to degrade MC-LR. Therefore, immobilized FeTPyPY was available to break down the toxic groups within MC-LR by utilizing environmental •OH radical under circumneutral condition.
Dziedzictwo oraz bilans praktyki i myśli pedagogicznej w Drugiej Rzeczypospolitej
Authors: Jakubiak Krzysztof , Jamrożek Wiesław
Published: February 2020

Artykuł stanowi próbę oceny i prezentacji – w formie syntetycznej – osiągnięć i dokonań praktyki i myśli (teorii) pedagogicznej Polski międzywojennej. Przedstawione są w nim określone działania i fakty związane z budową podstaw systemu edukacji narodowej w odrodzonej Polsce – po 1918 roku i kształtowaniem się w niej pedagogiki jako dyscypliny o statusie naukowym (teoretycznym) i autonomicznym.
Nauczyciel w początkach niepodległej Polski. „Szkoła Powszechna” o przymiotach, obowiązkach i profesjonalizacji wychowawców młodego pokolenia
Authors: Michalska Iwonna
Published: February 2020

„Szkoła Powszechna” była kwartalnikiem, który ukazywał się w Warszawie w latach 1920–1928. W trudnym, pierwszym po odzyskaniu państwowości, okresie tworzenia polskiego szkolnictwa pismo odgrywało rolę „informatora” na temat spraw wychowania i nauczania. Poważne w nim miejsce zajmowała problematyka pedeutologiczna. Zamieszczane w tym dziale treści oscylowały między teorią a praktyką pedagogiczno-dydaktyczną. Z jednej strony skupiano się na zarysowywaniu postulowanego „obrazu” nauczyciela wraz ze wskazywaniem jego pożądanych cech, niezbędnych do kierowania edukacją uczniów, a jednocześnie precyzowano liczne zadania stawiane przed wychowawcą klasowym, z drugiej strony koncentrowano się na przygotowaniu zawodowym nauczycieli. Brak stosownego wykształcenia wielu z nich powodowało, że redakcja pisma skupiała się na ukazywaniu dróg zdobywania kwalifikacji niezbędnych w nauczaniu powszechnym, treści na temat dokształcania i doskonalenia w wykonywanej profesji.
The Life of the Shaken
Authors: Svobodová Zuzana
Published: February 2020

Based on the analysis of texts by Jan Patočka, the author explores two concepts of human existence. The concept of three movements of life and the concept of two basic forms of life are examined in this paper, with the aim to referring to similarities and differences between them and to try to point out the essentials from these conceptions. The motivating question that gives rise to author´s efforts in this paper is: “What kind of agreement can be found between different concepts?”
Reclaiming a Dwelling Place: How a German History Learning Community Handles Traumatic Memories of Shoah Witnesses
Authors: Morgan Katalin Eszter
Published: February 2020

This paper describes the results of an empirical study in which the objective was to find out how traumatic narratives by Shoah survivors are handled in a classroom setting. The study was conducted using an ethnographic approach by means of which a history lesson sequence was observed and analysed. The teaching medium of the lesson was a digitised presentation of memories by Shoah survivors. The description of the lesson establishes how teachers and students negotiate the difficult terrain of an emotional engagement with the testimonies. The findings are discussed by elaborating on the meaning of traumatic memories for the witnesses, and in turn by asking what it means to listen to the telling of such memories. Conclusions are drawn about the overall meaning of (history) education.
Teaching English with Insta.Ling Online Platform: Case Study in Higher Education Level
Authors: Baranowski Artur
Published: February 2020

The paper presents a study about using Insta.Ling e-learning platform in university teaching process of English language for Biology students. The aim of this study is to estimate its effectiveness and the correlation between the number of repetitions and the number of words memorized. The total number of students involved in this research is 129 (6 student groups and in 3 academic years). They are all students of Cardinal Stefan Wyszynski University, Warsaw. The results allow to assume that Insta.Ling environment is an effective way to learn new vocabulary and regular sessions positively affect the number of vocabulary memorized.
Inkluzywna edukacja religijna na przykładzie systemu szkolnictwa w Niemczech
Authors: Zduniak Agnieszka
Published: February 2020

W artykule zostają przedstawione główne założenia inkluzywnej edukacji religijnej w niemieckim systemie szkolnictwa. Heterogeniczność kulturowa i etniczna współczesnych społeczeństw europejskich stanowi ważne wyzwanie dla systemu edukacji. Dotyczy to również nauczania religii w warunkach pluralizmu religijno-światopoglądowego. W Niemczech nauczanie tego przedmiotu cechuje duża różnorodność podejść i koncepcji, uzasadniona sytuacją religijnego i kulturowego pluralizmu. Rezultatem tego jest dynamiczny rozwój pedagogiki religii jako dziedziny wiedzy odrębnej od katechetyki. Autorka przedstawia najnowsze koncepcje szkolnego nauczania religii w Niemczech. Stanowią one próbę zdefiniowania na nowo celów i metod nauczania religii w taki sposób, aby w większym niż dotychczas stopniu odpowiadało ono wymogom współczesności.
Podręczniki do nauki islamu używane przez Muzułmański Związek Religijny w RP a specyfika muzułmańskiej edukacji religijnej w Polsce –między Polską a światem muzułmańskim
Authors: Nalborczyk Agata S.
Published: February 2020

Muzułmański Związek Religijny (MZR) jest najstarszą organizacją muzułmańską Polsce – został założony przez Tatarów – muzułmanów polsko-litewskich w 1925 roku. Po wprowadzeniu nauki religii muzułmańskiej do szkół w 1919 roku i po przywróceniu jej tam w 1992 roku jednym z głównych problemów zawsze był brak odpowiednich podręczników do nauki tego przedmiotu.
Mimo że niektóre nowe podręczniki zostały opublikowane po 1990 roku, nie obejmują one całego programu edukacji religijnej, a liczba egzemplarzy jest niewystarczająca, dlatego też podręczniki sprzed 1939 roku są nadal w użyciu, nawet jeśli są przestarzałe. Większość nowych podręczników, opublikowanych w ostatnich latach, to tłumaczenia z języków obcych – z bośniackiego, tureckiego (przez rosyjski lub angielski) lub arabskiego.
Artykuł omawia używane przed II wojną światową, obecnie przez nauczycieli MZR, podręczniki do nauki religii muzułmańskiej, koncentrując się na ich pochodzeniu, formie i roli języka arabskiego, a także metodyce jego nauczania.
Overcoming sexual addiction: peculiarity, dynamics of development, consequences and therapy
Authors: Kurlak Iryna
Published: February 2020

The goal of this article is to show the peculiarity of sexual addiction – a manifestation of behavioral addiction – characteristics, specificity, dynamics of development, degrees of sex addiction, negative consequences for a person and her or his environment, families and relatives, and forms of therapy.
Przywiązanie podstawą wychowania rodzinnego do relacji
Authors: Potočárová Mária
Published: February 2020

Autorka z perspektywy interdyscyplinarnej prezentuje wybrane teorie wyjaśniające fenomen przywiązania i odczytuje go w kontekście wychowania rodzinnego. Przez to eklektyczne postępowanie zmierza do wyodrębnienia wiedzy adekwatnej do potrzeb współczesnych rodziców, których kompetencje w zakresie relacji wewnątrzrodzinnych wydają się niewystarczające i wymagają podjęcia działania pedagogicznego.

Artykuł składa się z pięciu sekcji. W pierwszej przedmiotem analizy jest fenomen przywiązania w ujęciu teorii J. Bowlby’ego. W drugiej sekcji zidentyfikowano go w wychowaniu rodzinnym. Trzecia sekcja zawiera wskazania odnośnie sposobu kształtowania relacji przywiązania przez wychowanie rodzinne. W czwartej i piątej ten sam problem rozważany jest od strony rozwoju osobowości i socjalizacji rodzinnej.
Kryzys (w) edukacji i jego przezwyciężenie według Jana Amosa Komeńskiego i Emanuela Rádla
Authors: Pelcová Naděžda
Published: February 2020

Wychowanie i kształcenie są współcześnie pogrążone w głębokim kryzysie. Żeby zrozumieć jego istotę i możliwości przezwyciężenia, autorka odwołuje się do myśli Jana Amosa Komeńskiego i Emanuela Rádla. Pomimo dystansu czasowego ich twórczość pedagogiczna pozostaje wciąż aktualna i inspirująca.
Artykuł składa się z trzech części. W pierwszej zrekonstruowano poglądy Komeńskiego odnośnie wychowania jako swoistego sposobu oddziaływania pedagogicznego i znaczenie wychowania w edukacyjnym procesie dochodzenia do pełni człowieczeństwa – tak przez jednostkę, jak i społeczeństwo. Druga część dotyczy postaci Rádla, XX-wiecznego przyrodoznawcy i filozofa, który – podobnie jak Komeński – zauważał problemy (w) edukacji i zastanawiał się nad sposobami ich rozwiązania. Na zakończenie skonfrontowano stanowiska obu myślicieli, poszukując inspiracji do przezwyciężenia współczesnego kryzysu (w) edukacji.
Człowiek–kształcenie–przyroda w edukacji leśnej
Authors: Tuszyńska Ligia , Klimski Marcin
Published: February 2020

Przedmiotem artykułu jest edukacja leśna, odczytana jako forma kształcenia dotycząca współzależności między człowiekiem a przyrodą. Autorzy stawiają sobie za cel ukazanie alternatywnego charakteru edukacji leśnej wobec nauczania prowadzonego w tradycyjny sposób. Pierwszą część artykułu tworzą rozważania ukazujące relację człowieka i przyrody. To uzasadnia nie tylko potrzebę ochrony środowiska rozumianej jako troska o zasoby naturalne, lecz także edukacji w tym zakresie. W części drugiej przybliżona zostanie edukacja dla zrównoważonego rozwoju w kontekście Agendy 2030. W trzeciej części scharakteryzowano edukację leśną na przykładzie działalności Centrum Edukacji Przyrodniczo-Leśnej w Warszawie.
Dobrostan psychiczny a poziom odczuwanego stresu i satysfakcji ze studiów w doświadczeniach studentów pedagogiki przedszkolnej i wczesnoszkolnej
Authors: Kulawska Ewa
Published: February 2020

Celem artykułu jest opisanie korelatów dobrostanu psychicznego studentów pedagogiki przedszkolnej i wczesnoszkolnej kończących studia licencjackie lub magisterskie. Przeprowadzono badania empiryczne metodą sondażu diagnostycznego wśród 400 studentów z wybranych uczelni w Polsce. Zastosowano trzy narzędzia badacze: kwestionariusz WEMWBS (Tennant, Stewart-Brown) – do pomiaru dobrostan psychicznego, skalę PSS-10 (Cohen, Kamarck, Mermelstein) – do określenia poziomu odczuwanego stresu, kwestionariusz AS (Lent i in.) – do pomiaru stopnia zadowolenia ze studiów. Badania przeprowadzono w pierwszym kwartale 2019 roku. Analizy statystyczne wyników badań wykazały, że większość badanych studentów prezentuje przeciętny poziom dobrostanu psychicznego, wysoki poziom stresu i średnie zadowolenie ze studiów. Na podstawie analizy regresji hierarchicznej ustalono, że predyktorami dobrostanu psychicznego jest poziom odczuwanego stresu, wsparcia społecznego i satysfakcji ze studiów. Trzy predyktory wyjaśniają 38 proc. wariancji dobrostanu psychicznego. Wraz ze wzrostem stresu obniża się poziom dobrostanu psychicznego, a wyższe poczucie satysfakcji ze studiów i wsparcia społecznego pozytywnie wpływają na dobrostan psychiczny studentów pedagogiki przedszkolnej i wczesnoszkolnej.
What do Youth Really Care for? Towards Ethical Education, Education for Sustainable Development and Youth Values
Authors: Sádovská Anna
Published: February 2020

Artykuł dotyczy pytania: w jaki sposób można włączyć edukację na rzecz zrównoważonego rozwoju w nauczanie i uczenie się szkolne i powiązać z wychowaniem moralnym i kształceniem etycznym? Autorka przedstawia wyniki badania dotyczącego wartości i postaw proekologicznych uczniów, które przeanalizowała w powiązaniu z deklarowanymi przez nich zachowaniami prospołecznymi i tak zwanym pesymizmem społecznym. Dzięki analizie stwierdziła zachodzenie znaczącej korelacji między postawą hedonistyczną a uniwersalistyczną oraz między deklarowanymi zachowaniami prospołecznymi a uniwersalizmem światopoglądowym. Ponadto okazało się, że niższy poziom skali pesymizmu społecznego koreluje z wyższym poziomem skali uniwersalizmu.
Leadership in Educational Institutions: Shared Duty and Joint Responsibility
Authors: Pękala Joanna Ludwika , Kowalska Wiktoria
Published: February 2020

In the present world changes are inevitable and modern schools will also be presented to them. School principals should, therefore, aim at transforming the institutions into learning organizations, thus gathering people capable of self-reflection and self-improvement. The purpose of this article is to prove that in order to achieve measurable and sustainable success in an educational institution, leadership must be divided between the principal and the teachers. The ability of diagnosing the situation of the institution is also important, as is the drawing of constructive conclusions from the introduced changes, both those successful and those unsuccessful. Knowledge of possible leadership styles can be an inspiration to search for effective solutions in educational practice for headteachers, teachers and external experts who provide support to schools in the process of change.
Features of ‘New’ Students and the Potential of Future Teachers: First Effects of “The Unteachables” International Project
Authors: Kulpa-Puczyńska Aleksandra
Published: February 2020

The problem of students with difficulties in functioning in a school environment is nowadays becoming significant, as the environment often does not respond to unique and complex needs of young learners, such as modern ways of exchanging experience and knowledge, or the possibility of active participation in the community of learners and support networks. This state of affairs dictates the need to create new educational resources (also developed on the basis of cooperation of the academic community with secondary school teachers, or the cooperation of experts and practitioners from various countries) for future teachers who may face, for example, the problem of “burnt-out” young students. The main purpose of this paper is to share the first research results and reflections concerning the implementation of “The Unteachables” Erasmus+ project at the Faculty of Education and the Faculty of Biology and Environmental Sciences at the Cardinal Stefan Wyszynski University in Warsaw (in cooperation with partners from Denmark – project coordinator, Iceland, Spain, Slovenia and Italy).
ANTHROPOGENIC POLLUTANTS: 10 YEARS OF PROGRESS IN ECOTOXICOLOGICAL STUDIES AND AQUATIC RISK ASSESSMENT
Authors: Gheorghe Stefania , Lucaciu Irina , Paun Iuliana , Vasile Gabriela Geanina , Petre Jana , Iancu Vasile Ion , Stoica Catalina , Mitru Daniel , Nita-Lazar Mihai
Published: February 2020

The market expansion due to globalization generated more anthropogenic contaminants, enhancing their negative impact on the environment. In order to prevent and balanced their negative effect, more rapid, sensitive and eco-friendly ecotoxicological studies as risk assessments were developed. Each country developed national programs to ensure the research infrastructure for implementation and development of new testing methodologies, generating new experimental data transferable to governmental authorities, economic agents and academia. In this review, we intend to highlighted the progress of Romanian researchers in the last 10 years in the field of ecotoxicology and aquatic risk assessment. The studies focused on aquatic systems as a non-targeted system to the anthropogenic pollutants impact. The implemented methodologies according with international requirements for chemical control like REACH Regulation, Detergents Regulation, Biocide Products Regulation or the Aquatic Risk Assessment Guidelines were presented. The ecotoxicological effects of various contaminants such as detergents, pharmaceuticals, pesticides, biocides or heavy metals and environmental polluted samples (surface water, sediment or sludge) were provided. Overall, the studies presented in our studies have a nationally novelty through the variety of test substances, the complexity of laboratory experiments and data processing. The research work has a continue applicability in industry and for control authorities. New accredited testing services that providing useful information about the chemicals and their impact on the environmental were introduced. In addition, the ecotoxicology studies and their challenges were a great source for professional training like PhD and master studies and for impact published papers.
OCCURRENCE OF PHARMACEUTICALS AND DISINFECTANTS IN THE DISSOLVED WATER PHASE OF THE DANUBE RIVER AND THREE MAJOR TRIBUTARIES FROM ROMANIA
Authors: Petre Jana , Iancu Vasile Ion , Galaon Toma , Simion Marius , Niculescu Marcela , Vasile Gabriela Geanina , Pascu Luoana Florentina , Lehr Carol Blaziu , Cruceru Liliana , Calinescu Simona , Radu Gabriela Lucian
Published: February 2020

The presence of pharmaceuticals in the aquatic ecosystem has received great attention from the scientific community in the last decades, due to their potential impact on living organisms. This paper presents a short review of the results of investigations performed by INCD-ECOIND concerning the occurrence of 32 pharmaceutical compounds belonging to important therapeutic classes and 2 disinfectants along the Danube River and its tributaries. Grab water samples were collected from multiple points along the River (10 sites) and from 2 locations for each of the tributaries Jiu, Olt and Argeș, upstream and downstream of large cities. All samples were quantified via solid phase extraction, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results of these studies show that various pharmaceutical compounds are present in the dissolved water phase of the Danube River and its tributaries in low to moderate amounts with variation due to season and location.
Uterine Dnmt3a is not Required for Mouse Embryo Implantation
Authors: Li Na , Lu Siyu , Ding Yubin , Chen Xuemei , He Junlin , Liu Xueqing , Chen Mengyue , Zhang Juanjuan , Su Yan , Zhang Xue , Wang Yingxiong , Gao Rufei
Published: February 2020

Background:

Recent studies have demonstrated that endometrial DNA
methylation is essential for embryo implantation during early pregnancy. Dnmt3a is one
of the key enzymes for DNA methylation and could be expressed in the endometrium
regularly at this stage.

Objective and Methods:

In this study, we conditionally ablated uterine Dnmt3a using
progesterone receptor-cre (Pgrcre) to define the physiological roles of Dnmt3a in female
reproduction.

Results:

We found that ovarian function was not apparently altered and the number of
embryo implantation sites in Dnmt3aloxP/loxP Pgrcre/+ (cKO) was not significantly varied
during early pregnancy. Western blotting and immunohistochemistry results showed no
difference in expression or location of the estrogen receptor α (ERα) and mucin 1
(Muc1), the marker of uterine receptivity. Although the expression of decidual markers,
matrix metalloproteinase-2 (Mmp2), matrix metalloproteinase-9(Mmp9), and bone
morphogenetic protein-2 (Bmp2), was slightly decreased in Dnmt3a cKO females, the
gross morphology of mice uteri during decidualization was not significantly influenced. In
the artificial induction of the decidualization model, there was also no remarkable
difference in visually observed morphology or uterine weight in Dnmt3a cKO. Lastly, a
continuous breeding study showed that the fertility of Dnmt3a cKO female mice was not
strikingly altered.

Conclusion:

Overall, these results demonstrated that although some decidual markers
are expressed abnormally, conditional knockout of Dnmt3a in the uterus did not
significantly affect the endometrial function during embryo implantation; the embryo
could implant into the endometrium normally.
Impact of Ultrasound Therapy on Stem Cell Differentiation - A Systematic Review
Authors: Amini Abdollah , Chien Sufan , Bayat Mohammad
Published: February 2020

Objective:

This is a systematic review of the effects of low-intensity pulsed ultrasound
(LIPUS) on stem cell differentiation.

Background Data:

Recent studies have investigated several types of stem cells from different sources
in the body. These stem cells should strictly be certified and promoted for cell therapies before being
used in medical applications. LIPUS has been used extensively in treatment centers and in research to
promote stem cell differentiation, function, and proliferation.

Materials and Methods:

The databases of PubMed, Google Scholar, and Scopus were searched for
abstracts and full-text scientific papers published from 1989-2019 that reported the application of
LIPUS on stem cell differentiation. Related English language articles were found using the following
defined keywords: low-intensity pulsed ultrasound, stem cell, differentiation. Criteria for inclusion in
the review were: LIPUS with frequencies of 1–3 MHz and pulsed ultrasound intensity of <500
mW/cm2. Duration, exposure time, and cell sources were taken into consideration.

Results:

Fifty-two articles were selected based on the inclusion criteria. Most articles demonstrated that
the application of LIPUS had positive effects on stem cell differentiation. However, some authors recommended
that LIPUS combined with other physical therapy aides was more effective in stem cell
differentiation.

Conclusions:

LIPUS significantly increases the level of stem cell differentiation in cells derived mainly
from bone marrow mesenchymal stem cells. There is a need for further studies to analyze the effect of
LIPUS on cells derived from other sources, particularly adipose tissue-derived mesenchymal stem cells,
for treating hard diseases, such as osteoporosis and diabetic foot ulcer. Due to a lack of reporting on standard
LIPUS parameters in the field, more experiments comparing the protocols for standardization of
LIPUS parameters are needed to establish the best protocol, which would allow for the best results.
Synthesis of Dihydrazones as Potential Anticancer and DNA Binding Candidates: A Validation by Molecular Docking Studies
Authors: Sridhara Malavalli B. , Rakesh Kadalipura P. , Manukumar Honnayakanahalli M. , Shantharam Chavalmane S. , Vivek Hamse K. , Kumara Humegowdeenahally K. , Mohammed Yasser H.E. , Gowda Dale C.
Published: February 2020

Background:

Accounting for mortality nearly one in four of human and second highest leading cause of death worldwide. Every year, about 10 million new cancers are diagnosed and causing major health issues in both developing and developed countries.

Methods:

A series of new dihydrazones were synthesized and screened for in vitro anticancer activity against three different MDA-MB-231, A546 and MCF7 cell lines and validated by DNA binding and molecular docking approaches.

Result:

In the present investigations, synthesized compounds 21, 22, 23 and 24 exhibited potent anticancer activity against tested cancer cell lines and DNA binding study using methyl green comparing to Doxorubicin and ethidium bromide as a positive control respectively.

Conclusion:

The Structure Activity Relationship (SAR) showed that the electron withdrawing groups (-Cl, -NO2, - F, and -Br) favored the DNA binding studies and anticancer activity whereas, electron donating groups (-OH and - OCH3) showed moderate activity. In the molecular docking study, binding interactions of the most active compounds 21, 22, 23 and 24 stacked with A-T rich regions of the DNA minor groove by surface binding interactions were confirmed. Further, the tuning of active analogs for targeted therapy was warranted.
Preparation and Characterization of Highly Fluorescent TGA-CdTe Quantum Dot-Hyamine 1622 Additive Composite
Authors: Kudrat-E-Zahan Md. , Yildiz Salih Zeki , Yazici Senem Colak
Published: February 2020

Objective:

The aim of the present study was to prepare highly luminescent additive composite
polymer with hyamine 1622 and Thioglycolic Acid (TGA) coated CdTe Quantum Dots (QDs).

Methods:

The additive nano-composite was synthesized by the colloid synthesis method for the first
time. The properties like particle size, fluorescence efficiency, fluorescence imaging, self-assembling,
quantum dots, encapsulation, etc. were characterized by the employing of instrumental techniques
such as 1H and 13C NMR, Fourier Transform Infrared spectroscopy (FT-IR), BAB image analysis system
spectroscopy, Atomic Force Microscopy (AFM), Transmission Electron Microscopy (TEM) and
Energy Dispersive X-Ray Spectroscopy (EDS).

Results:

CdTe quantum dots were stabilized successfully in the solid phase by hydrophobic conversion
with hyamine 1622 as the cationic surfactant. The experimental results show that the prepared
composite is ideal for various applications, easily synthesized, safe, and maintain good fluorescence
properties.

Conclusion:

The newly prepared additive nanocomposite having sharp and narrow excitation/
emission properties is expected to be applicable in biomedical/analytical systems.