bluuebunny/crossref_metadata_2025 huggingface dataset:
Low seroprevalence of equine piroplasmosis in horses exported from the Netherlands between 2015 and 2021
Authors: Graham Heather , van Kalsbeek Paul , van der Goot Jeanet , Koene Miriam G. J.
Published: October 2022

Equine piroplasmosis (EP) is a tick-borne disease affecting horses, donkeys, mules and zebras, caused by the intracellular apicomplexan protozoa Babesia caballi and Theileria equi. The geographical distribution of EP is closely related to the distribution of its vector tick species belonging to the genera of Dermacentor, Rhipicephalus and Hyalomma. Since the discovery of Dermacentor reticulatus ticks in 2007 and the first reported autochthonous cases in the South of the Netherlands in 2012, no data on the (sero)prevalence of EP in horses in the Netherlands have been reported and it remains unclear whether B. caballi and T. equi have been able to establish themselves in the Netherlands. This study aims to give an update on the current status of EP in horses in the Netherlands using data from serological tests performed in the context of export and screening of 12,881 horses from 2015 through 2020. Horses were categorized as “Dutch,” “Foreign,” or “Unknown” based on microchip number. The overall seroprevalence of EP in Dutch horses was found to be 0.5% (95% exact CI [0.4–0.7]), compared to 1.9% (95% exact CI [1.3–2.6]) in horses in the category “Foreign” and 1.7% (95% exact CI [1.2–2.3]) in horses in the category “Unknown.” In addition, the seroprevalence per country in the category “Foreign” ranged from 0% (0.95% exact CI [0–2.8]) for Ireland to 6.0% (0.95% exact CI [3.5–9.3]) for Spain. In light of the reports on the seroprevalence during the outbreak of autochthonous EP reported in 2012 and on seroprevalences of EP in other countries in Northwestern Europe, the seroprevalence of EP in horses exported from the Netherlands is very low. However, the higher seroprevalence of EP in horses from abroad warrants the need for the monitoring of EP, as tick vectors are present in the Netherlands and the import of horses from endemic areas increases the chances of EP becoming more prevalent in the Netherlands.
Serum amyloid A and fibrinogen as markers for early detection of surgical site infection associated with internal fixation in the horse
Authors: Thurston Catherine C. , Stefanovski Darko , MacKinnon Melissa C. , Chapman Hannah-Sophie , Richardson Dean W. , Levine David G.
Published: October 2022

The objective of this study was to determine the diagnostic ability of serum amyloid A (SAA) and fibrinogen for early detection of surgical site infection (SSI) after equine internal fixation. Horses undergoing internal fixation for fracture, arthrodesis, or osteotomy with internal fixation for limb deformity were included in the study. SAA and fibrinogen were measured on blood samples preoperatively and on days 1, 3, 5, 7, 10, and 14 postoperatively. Statistical analysis included use of Spearman's rank correlation, logistic regression, and calculating the area under the receiver operating characteristic (ROC) curve. SAA and fibrinogen measurements were both associated with SSI, with SAA being considered an excellent marker (area under the ROC curve 0.8) and fibrinogen being considered acceptable (<0.8). As the amount of time postoperatively increased, SAA elevations indicated a higher likelihood of SSI (area under the ROC curve 0.8 compared with fibrinogen 0.7). SAA and fibrinogen were predictive markers of SSI and SAA is of greater diagnostic utility when compared with fibrinogen. Persistent elevations of SAA postoperatively are associated with the development of SSI. Serial monitoring of SAA can be used to help predict the development of SSI in horses undergoing internal fixation. This may lead to earlier suspicion, and therefore recognition and treatment of SSI.
Management of bilateral pterygoid myositis ossificans-like lesion in dogs
Authors: De Paolo Mercedes , Gracis Margherita , Lacava Giuseppe , Vapniarsky Natalia , Arzi Boaz
Published: October 2022

Myositis ossificans (MO) and myositis ossificans-like lesions have been rarely described within the veterinary literature, and are even less common in the maxillofacial region. When MO affects the muscles of mastication, it can result in complete or partial inability to open the mouth. As with other conditions resulting in decreased or restricted mandibular range of motion, severe and potentially fatal sequelae such as difficulty with prehension, swallowing, and air exchange are possible. Diagnostic imaging is essential in achieving an accurate diagnosis and in formulating an appropriate treatment plan. In this “method” manuscript, we provide a detailed description of our approach to diagnosis and surgical management of MO-like lesions of the pterygoid muscles and describe our experience with two young French bulldogs.
Antrodia cinnamomea polysaccharide improves liver antioxidant, anti-inflammatory capacity, and cecal flora structure of slow-growing broiler breeds challenged with lipopolysaccharide
Authors: Ye Jinling , Zhang Chang , Fan Qiuli , Lin Xiajing , Wang Yibing , Azzam Mahmoud , Alhotan Rashed , Alqhtani Abdulmohsen , Jiang Shouqun
Published: October 2022

Lipopolysaccharides (LPS) induces liver inflammatory response by activating the TLR4/NF-κB signaling pathway. Antrodia cinnamomea polysaccharide (ACP) is a medicinal mushroom that can protect from intoxication, liver injury, and inflammation. Nevertheless, the effect of ACP on the liver antioxidant, anti-inflammatory capacity and cecal flora structure of LPS-challenged broilers remains unclear. The aim of this experiment was to investigate the effects of ACP on the anti-oxidative and anti-inflammatory capacities of the liver, and cecal microbiota in slow-growing broilers stimulated by LPS. A total of 750 slow-growing broilers (9-day-old) were assigned to five treatments with 6 replicates of 25 chicks per replicate: a control diet, the chicks were fed a control diet and challenged with LPS. Dietary treatments 3 to 5 were the control diet supplemented with 100, 200, 400 mg/kg ACP challenged with LPS, respectively. The groups of 100 mg/kg ACP supplementation significantly increased liver index, pancreas index, and bursa of Fabricius index (P < 0.05). The GSH-Px content of LPS-challenged broilers was lower than that of the control group (P < 0.001), but the content of MDA increased (P < 0.001). Feeding with 100 mg/kg ACP resulted in increased the activity of T-AOC, GSH-Px, and T-SOD, and decreased MDA content (P < 0.05). The activity of TNF-α, IL-1β, and IL-6 of the LPS group increased, but these indicators were decreased with supplemental 100 mg/kg ACP (P < 0.05). Dietary application of ACP up to 100 mg/kg down-regulated (P < 0.05) the expression of TLR4/NF-κB pathway in the liver induced by LPS. The results of 16S rRNA demonstrated that feeding with 100 mg/kg ACP can change the diversity and composition of the gut microbiota, and restrained the decline of beneficial cecal microbiota (typically Lactobacillus, Faecalibacterium, and Christensenellaceae R-7 group) in the challenged LPS group (P < 0.05). Conclusively, feeding a diet with 100 mg/kg ACP may have beneficial effects on liver damage and the bacterial microbiota diversity and composition in the ceca of LPS-stressed slow-growing broiler breeds, probably because of its combined favorable effects on antioxidants and cytokines contents, and restoration the decline of beneficial cecal microbiota.
Cysteamine-supplemented diet for cashmere goats: A potential strategy to inhibit rumen biohydrogenation and enhance plasma antioxidant capacity
Authors: Wu Tiecheng , Liang Jianyong , Wang Tao , Zhao Ruoyang , Ma Yuejun , Gao Yulin , Zhao Shengguo , Chen Guoshun , Liu Bin
Published: October 2022

Cysteamine (CS), as a feed supplement, can increase the level of growth hormone (GH) in the blood, promote animal growth. However, little attention has been paid to the effects of CS on the rumen microbiome and metabolic profile in cashmere goats. This study aimed to assess the effects of rumen microbiota, metabolites, and plasma antioxidative capacity induced by CS supplementation in cashmere goats. We selected 30 Inner Mongolia white cashmere goat ewes (aged 18 months), and randomly separate the goats into three groups (n = 10 per group) to experiment for 40 days. Oral 0 (control group, CON), 60 (low CS, LCS), or 120 mg/kg BW−1 (high CS, HCS) coated CS hydrochloride every day. Using 16S and internal transcribed spacer (ITS) rRNA gene amplicon sequencing, we identified 12 bacterial and 3 fungal genera with significant changes among the groups, respectively. We found a significant increase in rumen NH3-N and total volatile fatty acid (TVFA) concentrations in the LCS and HCS groups compared with the CON. With untargeted LC–MS/MS metabolomics, we screened 59 rumen differential metabolites. Among the screened metabolites, many unsaturated and saturated fatty acids increased and decreased with CS treatment, respectively. CS supplementation increased the levels of plasma total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), GH, and insulin-like growth factor-1(IGF-1). Spearman correlation analysis revealed that the abundance of U29-B03, Lactococcus, and Brochothrix were positively associated with the levels of δ2-THA, TVFA and antioxidant capacity. In conclusion, CS significantly affected rumen microbiota and fermentation parameters, and ultimately inhibited the biohydrogenation of rumen metabolites, enhanced plasma antioxidant capacity, and regulated some hormones of the GH–IGF-1 axis. This study provides an overall view into the CS application as a strategy to improve health production in cashmere goats.
Alcohol Screening and Brief Intervention in Primary Health Care in Kazakhstan—Results of a Cluster Randomised Pilot Study
Authors: Verthein Uwe , Lahusen Harald , Martens Marcus Sebastian , Prilutskaya Mariya , Yussopov Oleg , Kaliyeva Zhanar , Schulte Bernd
Published: October 2022

Objective: The aim of this pilot trial was to assess the feasibility of ASBI in primary health care units (PHCUs) in Kazakhstan.

Methods: A two-arm cluster randomised trial in five PHCUs based on the RE-AIM framework for implementation studies was carried out. Patients with AUDIT-C scores ≥4 for females and ≥5 for males received a brief face-to-face intervention delivered by a trained physician plus information leaflet (intervention group, IG) or simple feedback including a leaflet (control group, CG).

Results: Among 7327 patients eligible for alcohol screening according to the inclusion criteria 1148 patients were screened (15.7%, IG: 11.5%, CG: 27.3%). 12.3% (N = 141) were tested AUDIT-C positive (IG: 9.9%, CG: 15.1%). Out of 112 physicians invited, 48 took part in the ASBI training, 31 finally participated in the study, 21 in the IG (2 PHCUs), 10 in the CG (3 PHCUs). The majority of physicians did not have difficulties in performing the intervention.

Conclusion: ASBI is feasible and can be implemented into PHC settings in Kazakhstan. However, the implementation depends on the willingness and interest of the PHCU and the physicians.
The Impact of Industry Forces on International SME Performance in Iran: The Mediating Effect of Competitive Advantage
Authors: Sharfaei Shahab , Ong Jeen Wei , Ojo Adedapo O.
Published: October 2022

International small and medium-sized enterprises (SMEs) encounter several problems, including how to manage the industry forces in order to achieve their performance goals. Focusing mainly on international SMEs, we explored this issue by examining competitive advantage as a mediator in the relationship between industry forces and international SMEs’ performance. This study was conducted in the context of the Iranian market. Iran offers substantial economic opportunities to international SMEs since it has a population of 85 million people, a range of natural resources, and a sophisticated manufacturing industry. In Iran, an international SME is a firm with less than 100 employees that originated outside of Iran but has an entity inside the country. The partial least squares method was employed in analyzing the survey data collected from 166 international SMEs in Iran. The results indicate that industry forces have significant effects on performance, but competitive advantage does not have a mediating impact. The results of this study reaffirm that international SMEs should be aware of the consequences of industry forces in order to attain their performance goals. This is especially critical for some developing economies which are more characterized by ambiguities.
F1 Male Sterility in Cattle-Yak Examined through Changes in Testis Tissue and Transcriptome Profiles
Authors: Cao Mengli , Wang Xingdong , Guo Shaoke , Kang Yandong , Pei Jie , Guo Xian
Published: October 2022

Male-derived sterility in cattle-yaks, a hybrid deriving from yak and cattle, is a challenging problem. This study compared and analyzed the histomorphological differences in testis between sexually mature yak and cattle-yak, and examined the transcriptome differences employing RNA-seq. The study found that yak seminiferous tubules contained spermatogenic cells at all levels, while cattle-yak seminiferous tubules had reduced spermatogonia (SPG) and primary spermatocyte (Pri-SPC), fewer secondary spermatocytes (Sec-SPC), an absence of round spermatids (R-ST) and sperms (S), and possessed large vacuoles. All of these conditions could have significantly reduced the volume and weight of cattle-yak testis compared to that of yak. RNA-seq analysis identified 8473 differentially expressed genes (DEGs; 3580 upregulated and 4893 downregulated). GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment evaluations for DEGs found their relation mostly to spermatogenesis and apoptosis. Among the DEGs, spermatogonia stem cell (SSCs) marker genes (Gfra1, CD9, SOHLH1, SALL4, ID4, and FOXO1) and genes involved in apoptosis (Fas, caspase3, caspase6, caspase7, caspase8, CTSK, CTSB and CTSC) were significantly upregulated, while differentiation spermatogenic cell marker genes (Ccna1, PIWIL1, TNP1, and TXNDC2) and meiosis-related genes (TEX14, TEX15, MEIOB, STAG3 and M1AP) were significantly downregulated in cattle-yak. Furthermore, the alternative splicing events in cattle-yak were substantially decreased than in yak, suggesting that the lack of protein subtypes could be another reason for spermatogenic arrest in cattle-yak testis.
Machine Learning-Based Co-Expression Network Analysis Unravels Potential Fertility-Related Genes in Beef Cows
Authors: Diniz Wellison J. S. , Banerjee Priyanka , Rodning Soren P. , Dyce Paul W.
Published: October 2022

Reproductive failure is still a challenge for beef producers and a significant cause of economic loss. The increased availability of transcriptomic data has shed light on the mechanisms modulating pregnancy success. Furthermore, new analytical tools, such as machine learning (ML), provide opportunities for data mining and uncovering new biological events that explain or predict reproductive outcomes. Herein, we identified potential biomarkers underlying pregnancy status and fertility-related networks by integrating gene expression profiles through ML and gene network modeling. We used public transcriptomic data from uterine luminal epithelial cells of cows retrospectively classified as pregnant (P, n = 25) and non-pregnant (NP, n = 18). First, we used a feature selection function from BioDiscML and identified SERPINE3, PDCD1, FNDC1, MRTFA, ARHGEF7, MEF2B, NAA16, ENSBTAG00000019474, and ENSBTAG00000054585 as candidate biomarker predictors of pregnancy status. Then, based on co-expression networks, we identified seven genes significantly rewired (gaining or losing connections) between the P and NP networks. These biomarkers were co-expressed with genes critical for uterine receptivity, including endometrial tissue remodeling, focal adhesion, and embryo development. We provided insights into the regulatory networks of fertility-related processes and demonstrated the potential of combining different analytical tools to prioritize candidate genes.
Air Pollution Dispersion Modelling in Urban Environment Using CFD: A Systematic Review
Authors: Pantusheva Mariya , Mitkov Radostin , Hristov Petar O. , Petrova-Antonova Dessislava
Published: October 2022

Air pollution is a global problem, which needs to be understood and controlled to ensure a healthy environment and inform sustainable development. Urban areas have been established as one of the main contributors to air pollution, and, as such, urban air quality is the subject of an increasing volume of research. One of the principal means of studying air pollution dispersion is to use computational fluid dynamics (CFD) models. Subject to careful verification and validation, these models allow for analysts to predict air flow and pollution concentration for various urban morphologies under different environmental conditions. This article presents a detailed review of the use of CFD to model air pollution dispersion in an urban environment over the last decade. The review extracts and summarises information from nearly 90 pieces of published research, categorising it according to over 190 modelling features, which are thematically systemised into 7 groups. The findings from across the field are critically compared to available urban air pollution modelling guidelines and standards. Among the various quantitative trends and statistics from the review, two key findings stand out. The first is that, despite the existence of best practice guidelines for pollution dispersion modelling, anywhere between 12% and 34% of the papers do not specify one or more aspects of the utilised models, which are required to reproduce the study. The second is that none of the articles perform verification and validation according to accepted standards. The results of this review can, therefore, be used by practitioners in the field of pollution dispersion modelling to understand the general trends in current research and to identify open problems to be addressed in the future.
The Hourly Energy Consumption Prediction by KNN for Buildings in Community Buildings
Authors: Hong Goopyo , Choi Gyeong-Seok , Eum Ji-Young , Lee Han Sol , Kim Daeung Danny
Published: October 2022

With the development of metering technologies, data mining techniques such as machine learning have been increasingly used for the prediction of building energy consumption. Among various machine learning methods, the KNN algorithm was implemented to predict the hourly energy consumption of community buildings composed of several different types of buildings. Based on the input data set, 10 similar hourly energy patterns for each season in the historic data sets were chosen, and these 10 energy consumption patterns were averaged. The prediction results were analyzed quantitatively and qualitatively. The prediction results for the summer and fall were close to the energy consumption data, while the results for the spring and winter were higher than the energy consumption data. For accuracy, a similar trend was observed. The values of CVRMSE for the summer and fall were within the acceptable range of ASHRAE guidelines 14, while higher values of CVRMSE for the spring and winter were observed. In sum, the total values of CVRMSE were within the acceptable range.
LINC01526 Promotes Proliferation and Metastasis of Gastric Cancer by Interacting with TARBP2 to Induce GNG7 mRNA Decay
Authors: Zhou Jin-Yong , Liu Jin-Yan , Tao Yu , Chen Chen , Liu Shen-Lin
Published: October 2022

Gastric cancer is the most common malignancy of the human digestive system. Long noncoding RNAs (lncRNAs) influence the occurrence and development of gastric cancer in multiple ways. However, the function and mechanism of LINC01526 in gastric cancer remain unknown. Herein, we investigated the function of LINC01526 with respect to the malignant progression of gastric cancer. We found that LINC01526 was upregulated in gastric cancer cells and tissues. The function experiments in vitro and the Xenograft mouse model in vivo proved that LINC01526 could promote gastric cancer cell proliferation and migration. Furthermore, LINC01526 interacted with TAR (HIV-1) RNA-binding protein 2 (TARBP2) and decreased the mRNA stability of G protein gamma 7 (GNG7) through TARBP2. Finally, the rescue assay showed that downregulating GNG7 partially rescued the cell proliferation inhibited by LINC01526 or TARBP2 silencing. In summary, LINC01526 promoted gastric cancer progression by interacting with TARBP2, which subsequently degraded GNG7 mRNA. This study not only explores the role of LINC01526 in gastric cancer, but also provides a laboratory basis for its use as a new biomarker for diagnosis and therapeutic targets.
Combination of a Novel Fusion Protein CD3εζ28 and Bispecific T Cell Engager Enhances the Persistance and Anti-Cancer Effects of T Cells
Authors: Yu Feng , Gao Yang , Wu Yan , Dai Anran , Wang Xiaoyan , Zhang Xiangzhi , Liu Guodong , Xu Qinggang , Chen Dongfeng
Published: October 2022

Bi-specific T cell engager (BiTE), an artificial bi-functional fusion protein, has shown promising therapeutic potential in preclinical and clinical studies. However, T cells cannot be sufficiently activated by BiTE, most likely due to lacking co-stimulatory signal. We reasoned that incorporating co-stimulatory signal might have the potential to enhance the T cell activation mediated by BiTE. We, therefore, designed a chimeric fusion protein, named as CD3εζ28, which consists of the CD3ε extracellular region, the CD28 costimulatory signal and the intracellular region of CD3ζ in tandem. T cells genetically modified to express both CD3εζ28 and GFP (T-CD3εζ28-GFP) were generated by retroviral transduction. The results from in vitro experiments showed that T-CD3εζCD28-GFP cells had superior cytotoxic effects on tumor cells in presence of BiTE compared with control T cells, as evidenced by IL-2 and IFN-γ production, T cell proliferation and sequential killing assay. In vivo, T-CD3εζCD28-GFP cells showed superior anti-tumor effects in Hela-BiTE. EGFRvIII xenograft tumor model, as evaluated by tumor growth rate and T cell persistence in comparison with control T cells. In order to further confirm these findings, we generated T cells modified to express both CD3εζCD28 on cell surface and BiTE.CD19 by autocrine manner (T-CD3εζCD28-BiTE.19). The superior anti-tumor effects of T-CD3εζCD28-BiTE.19 cells could also be evidenced by the similar in vitro and in vivo experiments; thus, incorporating co-stimulatory signal may be an effective approach to improve the effector function of T cells mediated by BiTE.
Special Anaesthetic Considerations for Brain Tumour Surgery in Children
Authors: Jeker Sandra , Beck Maria Julia , Erb Thomas O.
Published: October 2022

Brain tumours are among the most common neoplasm in children. Therefore, paediatric anaesthesiologists face the challenge of neurosurgical interventions in all age groups. To minimize perioperative mortality and morbidity, a comprehensive understanding of age-dependent differences in anatomy and cerebrovascular physiology is a mandatory prerequisite. Advances in subspeciality training in paediatric neurosurgery and paediatric anaesthesia may improve clinical outcomes and advance communication between the teams.
Real-Time (iOCT) Guided Epiretinal Membrane Surgery Using a Novel Forceps with Laser-Ablated Microstructure Tip Surface
Authors: Ashurov Agharza , Chronopoulos Argyrios , Heim Julia , Schutz James Scott , Arndt Carl , Hattenbach Lars-Olof
Published: October 2022

Purpose: We investigated intraoperative OCT (iOCT)—guided epiretinal membrane (ERM) and internal limiting membrane (ILM) removal using a novel forceps with a laser-ablated tip surface; it was designed to help prevent indentation force, shear stress, or tractional trauma when grasping very fine membranes. Patients and Methods: This retrospective study included patients who underwent 23- and 25-gauge pars plana vitrectomy (PPV) for vitreoretinal interface disorders. ERM and ILM peeling was performed under guidance with microscope-integrated iOCT using novel ILM forceps with laser-ablated tip surfaces. These forceps were engineered to enhance friction when grasping tissue. Evaluation of ERM/ILM manipulation included postoperative slow-motion video analysis of the number of grasping attempts, initial ILM mobilization, and observed damage to retinal tissue. Results: ERM/ILM removal was successfully performed in all patients, with an average of four grasp actions to initial membrane mobilization (91%). Additional use of a diamond-dusted membrane scraper was used in two cases (9%). Mean best-recorded visual acuity (BRVA) logMAR improved from 0.5 ± 0.34 to 0.33 ± 0.36 (p = 0.05) and mean central retinal thickness (CRT) improved from 462 ± 146 µm to 359 ± 78 µm (p = 0.002). Postoperative iOCT video analysis demonstrated hyper-reflectivity of the inner retinal layers associated with retinal hemorrhage in five eyes (22%), but no grasping-related retinal breaks. Conclusions: The texturized surface on the tips of the ILM forceps were found to be helpful for mobilizing ILM edges from the retinal surface. iOCT-guided ERM surgery also allowed for improved intraoperative tissue visualization. We believe that these two technologies helped reduce both unnecessary surgical maneuvers and retinal damage.
Three-Dimensional Laser Scanning Technology Assisted Investigation and Extraction of Human Bone Information in Archaeological Sites at Shenna Ruins, China
Authors: Li Jiaxin , Li Kaiyuan , Zhao Fangnan , Feng Xue , Yu Jingli , Li Yuhu , Chao Xiaolian , Wang Juanli , Mai Bingjie , Cao Jing
Published: October 2022

Three-dimensional laser scanning technology can be used to quickly, efficiently, and accurately obtain spatial three-dimensional information of cultural relics without contacting the target during the scanning process. The results of this study showed that the extraction of human bones from the Shenna ruins via the auxiliary application of three-dimensional scanning technology reduced human intervention and destruction on the site compared with the traditional archaeological human bone packaging and extraction work method. When combined with the application of three-dimensional scanning technology, the original data information extracted on the spot were more comprehensive and accurate. Additionally, the technology provided us with important scientific data which can be used to discuss the phylogenetic composition of the ancient Qiang people in the settlement village, as well as a new applications of ideas for three-dimensional laser scanning technology usage in the field extraction of cultural relics. However, a follow-up study is needed to improve the comparisons of its applications, providing a conventional auxiliary means for cultural relic extraction and a technical means for cultural relic protection evaluation.
Reading Wishes from the Lips: Cancer Patients’ Need for Psycho-Oncological Support during Inpatient and Outpatient Treatment
Authors: Schulze Jan Ben , Dörner Marc , Usas Hermanas , Günther Moritz Philipp , von Känel Roland , Euler Sebastian
Published: October 2022

Background: Psycho-oncological support (PO) is an effective measure to reduce distress and improve the quality of life in patients with cancer. Currently, there are only a few studies investigating the (expressed) wish for PO. The aim of this study was to evaluate the number of patients who request PO and to identify predictors for the wish for PO. Methods: Data from 3063 cancer patients who had been diagnosed and treated at a Comprehensive Cancer Center between 2011 and 2019 were analyzed retrospectively. Potential predictors for the wish for PO were identified using logistic regression. As a novelty, a Back Propagation Neural Network (BPNN) was applied to establish a prediction model for the wish for PO. Results: In total, 1752 patients (57.19%) had a distress score above the cut-off and 14.59% expressed the wish for PO. Patients’ requests for pastoral care (OR = 13.1) and social services support (OR = 5.4) were the strongest predictors of the wish for PO. Patients of the female sex or who had a current psychiatric diagnosis, opioid treatment and malignant neoplasms of the skin and the hematopoietic system also predicted the wish for PO, while malignant neoplasms of digestive organs and older age negatively predicted the wish for PO. These nine significant predictors were used as input variables for the BPNN model. BPNN computations indicated that a three-layer network with eight neurons in the hidden layer is the most precise prediction model. Discussion: Our results suggest that the identification of predictors for the wish for PO might foster PO referrals and help cancer patients reduce barriers to expressing their wish for PO. Furthermore, the final BPNN prediction model demonstrates a high level of discrimination and might be easily implemented in the hospital information system.
Clinical Comparison of FD-CT and MS-CT in Aneurysmal Subarachnoid Haemorrhage: A Single Center Experience
Authors: Eisenhut Felix , Heidelbach Cornelius , Heynold Elisabeth , Manhart Michael , Struffert Tobias , Brandner Sebastian , Doerfler Arnd , Lang Stefan
Published: October 2022

Single-center comparison of postinterventional multislice computed tomography (MS-CT) and flat-detector computed tomography (FD-CT) in patients with subarachnoid haemorrhage (SAH) and endovascularly treated cerebral aneurysms with a focus on detection of posttherapeutical complications. Patients with endovascularly treated aneurysmal SAH undergoing both MS-CT and FD-CT within 24 h after intervention were included. Datasets were compared regarding image quality (IQ) as well as qualitative (detection of SAH, intracerebral haemorrhage [ICH], intraventricular haemorrhage [IVH], external ventricular drain [EVD] position, acute obstructive hydrocephalus [AOH]) and quantitative (cella media distance [CMD], modified Graeb score [GS]) parameters. 410 patients with endovascularly treated aneurysmal SAH were included. IQ was equal between MS-CT and FD-CT. FD-CT allowed equal detection of SAH and ICH in comparison to MS-CT. FD-CT allowed excellent detection of IVH and delineation of EVD position with strong agreement to MS-CT findings. FD-CT allowed equal detection of AOH in comparison to MS-CT. There was no significant difference of CMD and GS between FD-CT and MS-CT. Postinterventional FD-CT yields equivalent diagnostic value in patients with endovascular treated SAH as MS-CT. Enabling reliable detection of SAH-associated complications within the angiosuite, FD-CT might be an efficient and safe imaging modality in these clinical emergencies.
Tone Image Classification and Weighted Learning for Visible and NIR Image Fusion
Authors: Im Chan-Gi , Son Dong-Min , Kwon Hyuk-Ju , Lee Sung-Hak
Published: October 2022

In this paper, to improve the slow processing speed of the rule-based visible and NIR (near-infrared) image synthesis method, we present a fast image fusion method using DenseFuse, one of the CNN (convolutional neural network)-based image synthesis methods. The proposed method applies a raster scan algorithm to secure visible and NIR datasets for effective learning and presents a dataset classification method using luminance and variance. Additionally, in this paper, a method for synthesizing a feature map in a fusion layer is presented and compared with the method for synthesizing a feature map in other fusion layers. The proposed method learns the superior image quality of the rule-based image synthesis method and shows a clear synthesized image with better visibility than other existing learning-based image synthesis methods. Compared with the rule-based image synthesis method used as the target image, the proposed method has an advantage in processing speed by reducing the processing time to three times or more.
Impact of Leadership Behavior on Entrepreneurship in State-Owned Enterprises: Evidence from Civil Servant Management Aimed at Improving Accountability
Authors: Thuy Dao , Viet Truong , Phuc Vu , Pham Thi-Hong-Diep , Lan Nguyen , Ho Huong
Published: October 2022

This paper systematizes the theoretical foundation and empirical evidence of the impact of leadership behavior on entrepreneurship in state-owned enterprises, and the difference between state-owned enterprises that applied management based on the accountability of leaders, and those that did not. The paper uses the OLS regression model to identify the impact of leadership behavior on the entrepreneurship of state-owned enterprises by using data from a survey of 259 civil servants in Vietnamese state-owned enterprises. In our sample, 109 respondents belonged to the category of state-owned enterprises that did not apply management based on accountability, and 140 were state-owned enterprises that applied management based on accountability. The findings show that leadership behavior has a positive impact on the entrepreneurship of state-owned enterprises that do and do not apply management based on accountability, with results of 0.305 and 0.022, respectively. Moreover, the regression model is used to identify the factors that influence leadership behavior, including vision and wage policy having a statistical significance and positive impact on the leadership behaviors in all the state-owned enterprises. Additionally, some factors, including policy building, hi-tech usage, culture, teamwork, and training policy have positive impacts on leadership behavior in Model 1, and encouragement, monitoring, responsibility, and recruitment policy had positive impacts in Model 2 among the state enterprises. Additionally, this paper recommends some policies to promote leadership behavior in state enterprises in Vietnam.
Influences of Well Test Techniques and Uncertainty in Petrophysics on Well Test Results
Authors: Abdulaziz Abdulaziz M. , Ali Mohamed K. , Hafad Omalsaad F.
Published: October 2022

In the present study, well logs and well test data of both conventional build-up tests and Mini-DST from different oil and gas fields are utilized to evaluate the effects of uncertainty in petrophysics and test techniques on well test results. This includes producing wells from the Nile Delta and Western Desert-Egypt together with published results from West Qurna oil field-Iraq. Results indicated that permeability is strongly dependent on petrophysics interpretation, particularly pay thickness, while the radius of investigation is significantly dependent on fluid properties, especially compressibility. The skin factor calculations showed great sensitivity towards the pressure measurements with medium influences on porosity and oil viscosity. The calculations of Mini DST and Build-up test are compared within the uncertainty context for effective permeability, radius of investigation, and skin factor, and the findings are discussed in detail. In all cases, error analysis indicated that well test results and interpretation of conventional build up data are largely stable and may reduce overall uncertainty to 30% of the corresponding Mini-DST results/interpretation. The results of this study not only characterize each input parameter involved in the interpretation of well test data but also confirms the superiority of conventional build-up on Mini-DST techniques.
Recovery of Solid Waste in Industrial and Environmental Processes
Authors: Trinca Antonio , Segneri Valentina , Mpouras Thanasis , Libardi Nelson , Vilardi Giorgio
Published: October 2022

In recent years, an alarming increase in CO2 emissions has been noticed [...]
Equilibrium Seeking and Optimal Selection Algorithms in Peer-to-Peer Energy Markets
Authors: Ananduta Wicak , Grammatico Sergio
Published: October 2022

We consider a clearing problem in peer-to-peer energy markets, where prosumers can trade energy among each other and with the main grid to meet their energy demands. By using a game-theoretic formulation and exploiting operator-theoretic methods for generalized Nash equilibrium seeking, we propose two variants of the state-of-the-art distributed market clearing mechanism with improved convergence speeds. Furthermore, we design a third variant that allows for equilibrium selection, i.e., computing a specific market solution based on a convex preference function of the network operator, e.g., a congestion cost. We provide convergence guarantees and numerically show the advantages of our proposed algorithms in terms of convergence speed up and obtaining reduced grid congestion.
New Genetic Biomarkers of the Overlap Syndrome Tension-Type Headache and Arterial Hypertension
Authors: Alyabyeva Polina V. , Chastina Olga V. , Petrova Marina M. , Lareva Natalia V. , Garganeeva Natalia P. , Chumakova Galina A. , Cherniaeva Marina S. , Shnayder Natalia A.
Published: October 2022

Background: Nitric oxide (NO) is an important autocrine and paracrine signaling molecule that plays a crucial role in cardiovascular physiology and pathology regulation. NO is an important molecule involved in regulation of cerebral and extra cerebral cranial blood flow and arterial diameters. Reduced bioavailability of NO in the endothelium is an important precursor for impaired vasodilation and arterial hypertension (AH). Furthermore, NO is involved in nociceptive processing. A NO-induced biphasic response with immediate and a delayed headache is typical for chronic tension-type headaches (TTH) in humans. The aim was to study the association of allelic variants and genotypes of the single nucleotide variant (SNV) rs3782218 of the NOS1 gene with the TTH and AH overlap syndrome development in middle age adults. Materials and Methods: We observed 91 Caucasian participants who resided in Krasnoyarsk city: group 1 (TTH and AH overlap syndrome)—30 patients; group 2 (AH without headache)—30 patients; group 3 (control)—31 healthy volunteers. The diagnosis of AH was based on criteria of the European Society of Cardiology and the European Society of Hypertension (2018) и criteria of the Russian Society of Cardiology (2020). Diagnosis of TTH was based on criteria of the International Classification of Headache Disorders (2018). Real-time polymerase chain reaction was used for the determination of allelic variants and genotypes of the SNV rs3782218 of the NOS1 gene in all groups of participants. Results: The frequency of the minor allele T of rs3782218 was statistically significantly higher by 16.7 times in group 1 (TTH and AH) compared to group 3 (control): 26.7% versus 1.6%, respectively (p-value = 0.000065) and 3.2 times higher in group 1 (TTH and AH) compared to group 2 (AH without headache): 26.7% versus 8.3%, respectively (p-value = 0.008). The frequency of the heterozygous (CT) genotype was statistically significantly higher in group 1 (TTH and AH) compared to group 3 (control): 40.0% versus 3.2% (p-value = 0.000454) and in group 1 (TTH and AH) compared to group 2 (AH without headache): 40.0% versus 16.7% (p-value = 0.045). The minor allele T was statistically significantly associated with a high risk of developing the TTH and AH overlap syndrome compared with the controls (odds ratio (OR) = 22.2 (95% confidential interval (CI): 2.8–173.5)) and compared with AH without headache (OR = 4.0 (95% CI: 1.4–11.8)). Although the frequency of the minor allele T was 5.2 times higher in group 2 (AH without headache) compared with group 3 (control), there were not statistically significantly differences (p-value = 0.086). Conclusion: Thus, the minor allele T of rs3782218 of the NOS1 gene is an important genetic biomarker for a high risk of developing the TTH and AH overlap syndrome in hypertensive patients.
Study of Micro-Parameters of DEM Model on the Laboratory Experiment Results Obtained from Poorly Cemented Sandstone
Authors: Heo Jun Hyuk , Hashemi Sam S. , Melkoumian Noune
Published: October 2022

Borehole convergence during or after drilling is one of the primary indicators of borehole instability. Early recognition of borehole instability is critical in achieving successful and timely completion of drilling operations and for borehole exploitation. A series of numerical simulations was conducted to investigate the borehole convergence of poorly cemented sandstones using the Discrete Element Method (DEM). Rectangular 2-dimensional Particle Flow Code 2D (PFC2D) models were generated to study the effect of contributing micro-parameters such as stiffness ratio, friction angle, friction coefficient and effective modulus on the behaviour of poorly cemented sandstone subjected to triaxial compression tests. A good agreement between the calibrated numerical models and the experimental data obtained from the laboratory was observed. The results showed that the stiffness ratio was found to be a dominant factor in calibrating the numerical model, and the friction coefficient was the most influential micro-parameter.
Adipogenic Signaling Promotes Arrhythmia Substrates before Structural Abnormalities in TMEM43 ARVC
Authors: Vasireddi Sunil K. , Sattayaprasert Prasongchai , Yang Dandan , Dennis Adrienne T. , Bektik Emre , Fu Ji-dong , Mackall Judith A. , Laurita Kenneth R.
Published: October 2022

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a genetic disorder of desmosomal and structural proteins that is characterized by fibro-fatty infiltrate in the ventricles and fatal arrhythmia that can occur early before significant structural abnormalities. Most ARVC mutations interfere with β-catenin–dependent transcription that enhances adipogenesis; however, the mechanistic pathway to arrhythmogenesis is not clear. We hypothesized that adipogenic conditions play an important role in the formation of arrhythmia substrates in ARVC. Cardiac myocyte monolayers co-cultured for 2–4 days with mesenchymal stem cells (MSC) were derived from human-induced pluripotent stem cells with the ARVC5 TMEM43 p.Ser358Leu mutation. The TMEM43 mutation in myocyte co-cultures alone had no significant effect on impulse conduction velocity (CV) or APD. In contrast, when co-cultures were exposed to pro-adipogenic factors for 2–4 days, CV and APD were significantly reduced compared to controls by 49% and 31%, respectively without evidence of adipogenesis. Additionally, these arrhythmia substrates coincided with a significant reduction in IGF-1 expression in MSCs and were mitigated by IGF-1 treatment. These findings suggest that the onset of enhanced adipogenic signaling may be a mechanism of early arrhythmogenesis, which could lead to personalized treatment for arrhythmias associated with TMEM43 and other ARVC mutations.
IL-26 in the Lung and Its Role in COPD Inflammation
Authors: Bartziokas Konstantinos , Fouka Evangelia , Loukides Stelios , Steiropoulos Paschalis , Bakakos Petros , Papaioannou Andriana I.
Published: October 2022

IL-26 is a cytokine expressed by infiltrating pro-inflammatory IL-17-producing T cells in the tissues of patients with chronic lung inflammation. IL-26 induces the chemotactic response of human neutrophils to bacteria and other inflammatory stimuli. In recent years, the innovative properties of IL-26 have been described. Studies have shown that, as DNA is released from damaged cells, it binds to IL-26, which plays the role of a carrier molecule for extracellular DNA, further contributing to its binding to the site of inflammation. This mechanism of action indicates that IL-26 may serve both as a driver as well as a stimulus of the inflammatory process, leading to the installation of a noxious amplification loop and, eventually, persistent inflammation. IL-26 also demonstrates direct antimicrobial effects derived from its capability to create pores and disrupt bacterial membranes, as indicated by the presence of membrane blebs on the surface of the bacteria and cytosolic leakage pores in bacterial walls, produced in response to microbial stimuli in human airways by several different immune and structural cells. Surprisingly, while this particular cytokine induces the gathering of neutrophils in areas of infection, it also exhibits inhibitory and pro-inflammatory effects on airway epithelial and immune cells. These remarkable effects underline the necessity of a better understating of its biological behavior and its role in the pathophysiology and disease burden in several smoking-related airway inflammatory disorders, such as Chronic Obstructive Pulmonary Disease (COPD) and chronic bronchitis. In this review, we aim to discuss the current role of IL-26 in the lung, with an emphasis on systemic inflammation in patients suffering from COPD and chronic bronchitis.
The Implication of Misinformation and Stigma in Age-Related Quality of Life, Depression, and Coping Mechanisms of Adult Patients with Psoriasis
Authors: Decean Luminita , Badea Mihai , Rus Victoria , Buicu Gabriela , Sasu Andreea , Pilut Ciprian Nicolae , Mihai Adriana
Published: October 2022

Background and Objectives: Stigma and lack of acceptance in society might have detrimental effects on the quality of life of patients with psoriasis, sometimes being comparable with other chronic diseases and conditions that affect the appearance of a patient, such as burns. Therefore, we surveyed our patients diagnosed with psoriasis to determine the implications of misinformation and stigma for their quality of life, depression, and coping strategies stratified by different age categories. Materials and Methods: A cross-sectional study was designed for a sample size of a minimum of 45 patients considering a prevalence of psoriasis of 2–3% in the general population. The study participants (patients and controls) were given both a paper-based unstandardized questionnaire and an online version of three standardized surveys. The cohort of patients was further split into three age groups to determine their age-related quality of life and coping mechanisms. Results: The proportion of patients with a history of depression and depressive symptoms among patients with psoriasis was significantly higher. Multiple discrepancies were observed between patients and controls regarding questions that targeted stigma and misinformation. On the Coping Orientation to Problems Experienced Inventory (COPE-60) questionnaire, older patients were more likely to use positive coping mechanisms such as engagement and problem-focused coping, while the young patients were using more emotion-focused coping mechanisms. However, patients in the 30–50 age range group scored the highest on physical and mental health among all participants who filled the 12-Item Short Form Survey (SF-12) survey. The Dermatology Life Quality Index (DLQI) results showed significantly more patients answering “a lot and very much” concerning embarrassment and social activities, while sexual difficulties affected the older patients. The strongest correlations with depression were observed in the young patient group, who believed that psoriasis can cause skin cancer (rho = 0.418) and who had sexual difficulties (rho = 0.414) and embarrassment (rho = 0.359) as evaluated by the DLQI survey. In the 30- to 50-year-old group, the strongest correlations were with the feeling of being stigmatized (rho = 0.376), having sexual difficulties (rho = 0.367) and disengagement coping style (rho = 273). Conclusions: While the respondents are reasonably well-informed regarding psoriasis, a degree of stigma remains, likely due to involuntary emotional responses such as repulsion and embarrassment. It is essential to establish initiatives aimed at educating the general public, raising awareness, and establishing a more tolerant social environment for psoriasis patients.