bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations among Caesarean Section Birth, Post-Traumatic Stress, and Postpartum Depression Symptoms
Authors: Grisbrook Marie-Andrée , Dewey Deborah , Cuthbert Colleen , McDonald Sheila , Ntanda Henry , Giesbrecht Gerald F. , Letourneau Nicole
Published: April 2022

Caesarean section (C-section) deliveries account for nearly 30% of births annually with emergency C-sections accounting for 7–9% of all births. Studies have linked C-sections to postpartum depression (PPD). PPD is linked to reduced quality of parent-child interaction, and adverse effects on maternal and child health. New mothers’ perceptions of more negative childbirth experiences, such as unplanned/emergency C-sections, are linked to post-traumatic stress disorder (PTSD), which in turn is related to PPD. Our objectives were to determine: (1) the association between C-section type (unplanned/emergency vs. planned) and PPD symptoms, and (2) if postnatal PTSD symptoms mediate this association. Employing secondary analysis of prospectively collected data from 354 mother-child dyads between 2009 and 2013 from the Alberta Pregnancy Outcomes and Nutrition (APrON) study, conditional process modeling was employed. The Edinburgh Postnatal Depression Scale (EPDS) and the Psychiatric Diagnostic Screening Questionnaire (PDSQ) were administered at three months postpartum, to assess for postpartum depressive and post-traumatic stress symptoms. The direct effect of emergency C-section on PPD symptoms was non-significant in adjusted and non-adjusted models; however, the indirect effect of emergency C-section on PPD symptoms with PTSD symptoms as a mediator was significant after controlling for prenatal depression symptoms, social support, and SES (β = 0.17 (SE = 0.11), 95% CI [0.03, 0.42]). This suggests that mothers who experienced an emergency or unplanned C-section had increased PTSD scores of nearly half a point (0.47) compared to mothers who underwent a planned C-section, even after adjustment. Overall, emergency C-section was indirectly associated with PPD symptoms, through PTSD symptoms. Findings suggest that PTSD symptoms may be a mechanism through which emergency C-sections are associated with the development of PPD symptoms.
Mass Concentration, Source and Health Risk Assessment of Volatile Organic Compounds in Nine Cities of Northeast China
Authors: Shi Jianwu , Bao Yuzhai , Ren Liang , Chen Yuanqi , Bai Zhipeng , Han Xinyu
Published: April 2022

From April 2008 to July 2009, ambient measurements of 58 volatile organic compounds (VOCs), including alkanes, alkenes, and aromatics, were conducted in nine industrial cities (Shenyang, Fushun, Changchun, Jilin, Harbin, Daqing, Huludao, Anshan and Tianjin) of the Northeast Region, China (NRC). Daqing had the highest concentration of VOCs (519.68 ± 309.88 μg/m3), followed by Changchun (345.01 ± 170.52 μg/m3), Harbin (231.14 ± 46.69 μg/m3), Jilin (221.63 ± 34.32 μg/m3), Huludao (195.92 ± 103.26 μg/m3), Fushun (135.43 ± 46.01 μg/m3), Anshan (109.68 ± 23.27 μg/m3), Tianjin (104.31 ± 46.04 μg/m3), Shenyang (75.2 ± 40.09 μg/m3). Alkanes constituted the largest percentage (>40%) in concentrations of the quantified VOCs in NRC, and the exception was Tianjin dominated by aromatics (about 52.34%). Although alkanes were the most abundant VOCs at the cities, the most important VOCs contributing to ozone formation potential (OFP) were alkenes and aromatics. Changchun had the highest OFP (537.3 μg/m3), Tianjin had the lowest OFP (111.7 μg/m3). The main active species contributing to OFP in the nine cities were C2~C6 alkanes, C7~C8 aromatic hydrocarbons, individual cities (Daqing) contained n-hexane, propane and other alkane species. Correlation between individual hydrocarbons, B/T ratio and principal component analysis model (PCA) were deployed to explore the source contributions. The results showed that the source of vehicle exhausts was one of the primary sources of VOCs in all nine cities. Additionally, individual cities, such as Daqing, petrochemical industry was founded to be an important source of VOCs. The results gained from this study provided a large of useful information for better understanding the characteristics and sources of ambient VOCs incities of NRC. The non-carcinogenic risk values of the nine cities were within the safe range recognized by the U.S. Environmental Protection Agency (HQ < 1), and the lifetime carcinogenic risk values of benzene were 3.82 × 10−5~1.28 × 10−4, which were higher than the safety range specified by the US Environmental Protection Agency (R < 1.00 × 10−6). The results of risk values indicated that there was a risk of cancer in these cities.
Challenges in Working Conditions and Well-Being of Early Childhood Teachers by Teaching Modality during the COVID-19 Pandemic
Authors: Kwon Kyong-Ah , Ford Timothy G. , Tsotsoros Jessica , Randall Ken , Malek-Lasater Adrien , Kim Sun Geun
Published: April 2022

While a global understanding of teacher well-being during the COVID-19 pandemic is beginning to emerge, much remains to be understood about what early childhood teachers have felt and experienced with respect to their work and well-being. The present mixed-method study examined early care and education (ECE) teachers’ working conditions and physical, psychological, and professional well-being during the COVID-19 pandemic using a national sample of 1434 ECE teachers in the U.S. We also explored differences in working conditions and well-being among in-person, online, and closed schools, given the unique challenges and risks that ECE teachers may have faced by teaching in these different modalities. From the results of an online survey, we found that in the early months of the pandemic, many ECE teachers faced stressful, challenging work environments. Some were teaching in new, foreign modes and formats, and those still teaching in person faced new challenges. We found many common issues and challenges related to psychological and physical well-being across the three teaching groups from the qualitative analysis, but a more complicated picture emerged from the quantitative analysis. After controlling for education and center type, we found that aspects of professional commitment were lower among those teachers teaching in person. Additionally, there were racial differences across several of our measures of well-being for teachers whose centers were closed. Upon closer examination of these findings via a moderation analysis with teacher modality, we found that Black and Hispanic teachers had higher levels of psychological well-being for some of our indicators when their centers were closed, yet these benefits were not present for Black and Hispanic teachers teaching in person.
Problematic Social Media Use and Depressive Outcomes among College Students in China: Observational and Experimental Findings
Authors: Chen Yonghua , Liu Xi , Chiu Dorothy T. , Li Ying , Mi Baibing , Zhang Yue , Ma Lu , Yan Hong
Published: April 2022

Aims: Problematic social media use is increasing in China and could be a risk factor for depression. We investigated cross-sectional associations between problematic social media use and depressive outcomes among Chinese college students with potential mediation by perceived social support, social media violence, and loneliness. Thereafter, we evaluated the effectiveness of a one-month group counseling intervention in reducing depressive symptoms related to social media addiction. Methods: Depressive symptoms, social media addiction, perceived social support, social media violence, and loneliness were self-reported among 21,000 college students in Shaanxi province, China. A randomized controlled trial was designed based on the results of the observational study and Satir Transformational Systemic Therapy (STST) among 60 college students assigned to intervention (N = 30) or control/no treatment (N = 30). Self-administered surveys were completed at baseline (T1), at the end of the 1-month intervention (T2), and at 2-month follow-up post-intervention (T3). Results: After controlling for relevant covariates, more problematic social media use was associated with more depressive symptoms (β = 0.18, p < 0.001) and depression (OR = 1.08, 95% CI: 1.075, 1.092). Multiple mediation analyses found that perceived social support, social media violence, and loneliness significantly mediated associations between problematic social media use and depressive symptoms (model fit: RMSEA = 0.065, GFI = 0.984, CFI = 0.982). Bootstrapping revealed significant indirect effects of problematic social media use on depressive symptoms through the mediators named above (0.143, 95% CI: 0.133, 0.156). The subsequently informed intervention significantly reduced depressive symptoms at T2 (mean difference: −12.70, 95% CI: −16.64, −8.76, p < 0.001) and at T3 (mean difference: −8.70, 95% CI: −12.60, −4.80, p < 0.001), as well as levels of social media addiction, perceived social support, social media violence, and loneliness. Conclusions: Problematic social media use is a risk factor for depressive outcomes among Chinese college students, and perceived social support, social media violence, and loneliness mediate this association. STST-based group counseling may reduce depressive symptoms related to high social media usage in this population.
Toward User-Generated Content as a Mechanism of Digital Placemaking—Place Experience Dimensions in Spatial Media
Authors: Główczyński Maciej
Published: April 2022

Spatial media bring out new forms of interaction with places, leading to the emergence of new ways of embodying the experience. The perception of place and its dynamics of change has been multiplied by the emergence of digital platforms, which create many and varied representations of place in spatial media. These representations are dependent on the digital platforms’ ecosystem, formed by platform-specific mechanisms of digital placemaking. The study applied text mining techniques and statistical methods to explore the role of user-generated content as a digital placemaking practice in shaping place experience. The online reviews were collected from Google Maps for 23 places from Poznań, Poland. The analysis showed that place experience is described by three dimensions: attributes, practices and atmosphere, or place practices that most closely reflect the specificity of a place. The place attributes blurred the boundaries between their digital images, whereas the atmosphere dimension reduces the diversity and uniqueness of the place. In conclusion, user-generated content (UGC) as an element of the process of digital placemaking increases place awareness and democratizes human participation in its creation, yet it affects its reduction to homogeneous information processed through mechanisms operating within a given digital platform.
Structural Differences of PM2.5 Spatial Correlation Networks in Ten Metropolitan Areas of China
Authors: Zhang Shuaiqian , Tao Fei , Wu Qi , Han Qile , Wang Yu , Zhou Tong
Published: April 2022

The cross-impact of environmental pollution among cities has been reported in more research works recently. To implement the coordinated control of environmental pollution, it is necessary to explore the structural characteristics and influencing factors of the PM2.5 spatial correlation network from the perspective of the metropolitan area. This paper utilized the gravity model to construct the PM2.5 spatial correlation network of ten metropolitan areas in China from 2019 to 2020. After analyzing the overall characteristics and node characteristics of each spatial correlation network based on the social network analysis (SNA) method, the quadratic assignment procedure (QAP) regression analysis method was used to explore the influence mechanism of each driving factor. Patent granted differences, as a new indicator, were also considered during the above. The results showed that: (1) In the overall network characteristics, the network density of Chengdu and the other three metropolitan areas displayed a downward trend in two years, and the network density of Wuhan and Chengdu was the lowest. The network density and network grade of Hangzhou and the other four metropolitan areas were high and stable, and the network structure of each metropolitan area was unstable. (2) From the perspective of the node characteristics, the PM2.5 spatial correlation network all performed trends of centralization and marginalization. Beijing-Tianjin-Hebei and South Central Liaoning were “multi-core” metropolitan areas, and the other eight were “single-core” metropolitan areas. (3) The analysis results of QAP regression illustrated that the top three influencing factors of the six metropolitan areas were geographical locational relationship, the secondary industrial proportion differences, respectively, and patent granted differences, and the other metropolitan areas had no dominant influencing factors.
3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM)
Authors: Hansmeier Nils Rouven , Büschlen Ina Sophie , Behncke Rose Yinghan , Ulferts Sascha , Bisoendial Radjesh , Hägerling René
Published: April 2022

High-quality three-dimensional (3D) microscopy allows detailed, unrestricted and non-destructive imaging of entire volumetric tissue specimens and can therefore increase the diagnostic accuracy of histopathological tissue analysis. However, commonly used IgG antibodies are oftentimes not applicable to 3D imaging, due to their relatively large size and consequently inadequate tissue penetration and penetration speed. The lack of suitable reagents for 3D histopathology can be overcome by an emerging class of single-domain antibodies, referred to as nanobodies (Nbs), which can facilitate rapid and superior 2D and 3D histological stainings. Here, we report the generation and experimental validation of Nbs directed against the human endothelial cell-selective adhesion molecule (hESAM), which enables spatial visualization of blood vascular networks in whole-mount 3D imaging. After analysis of Nb binding properties and quality, selected Nb clones were validated in 2D and 3D imaging approaches, demonstrating comparable staining qualities to commercially available hESAM antibodies in 2D, as well as rapid and complete staining of entire specimens in 3D. We propose that the presented hESAM-Nbs can serve as novel blood vessel markers in academic research and can potentially improve 3D histopathological diagnostics of entire human tissue specimens, leading to improved treatment and superior patient outcomes.
Osteocytes: Their Lacunocanalicular Structure and Mechanoresponses
Authors: Moriishi Takeshi , Komori Toshihisa
Published: April 2022

Osteocytes connect with neighboring osteocytes and osteoblasts through their processes and form an osteocyte network. Shear stress on osteocytes, which is induced by fluid flow in the lacunae and canaliculi, has been proposed as an important mechanism for mechanoresponses. The lacunocanalicular structure is differentially developed in the compression and tension sides of femoral cortical bone and the compression side is more organized and has denser and thinner canaliculi. Mice with an impaired lacunocanalicular structure may be useful for evaluation of the relationship between lacunocanalicular structure and mechanoresponses, although their bone component cells are not normal. We show three examples of mice with an impaired lacunocanalicular structure. Ablation of osteocytes by diphtheria toxin caused massive osteocyte apoptosis, necrosis or secondary necrosis that occurred after apoptosis. Osteoblast-specific Bcl2 transgenic mice were found to have a reduced number of osteocyte processes and canaliculi, which caused massive osteocyte apoptosis and a completely interrupted lacunocanalicular network. Osteoblast-specific Sp7 transgenic mice were also revealed to have a reduced number of osteocyte processes and canaliculi, as well as an impaired, but functionally connected, lacunocanalicular network. Here, we show the phenotypes of these mice in physiological and unloaded conditions and deduce the relationship between lacunocanalicular structure and mechanoresponses.
Metastatic Voyage of Ovarian Cancer Cells in Ascites with the Assistance of Various Cellular Components
Authors: Uno Kaname , Iyoshi Shohei , Yoshihara Masato , Kitami Kazuhisa , Mogi Kazumasa , Fujimoto Hiroki , Sugiyama Mai , Koya Yoshihiro , Yamakita Yoshihiko , Nawa Akihiro , Kanayama Tomohiro , Tomita Hiroyuki , Enomoto Atsushi , Kajiyama Hiroaki
Published: April 2022

Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy and has a unique metastatic route using ascites, known as the transcoelomic root. However, studies on ascites and contained cellular components have not yet been sufficiently clarified. In this review, we focus on the significance of accumulating ascites, contained EOC cells in the form of spheroids, and interaction with non-malignant host cells. To become resistant against anoikis, EOC cells form spheroids in ascites, where epithelial-to-mesenchymal transition stimulated by transforming growth factor-β can be a key pathway. As spheroids form, EOC cells are also gaining the ability to attach and invade the peritoneum to induce intraperitoneal metastasis, as well as resistance to conventional chemotherapy. Recently, accumulating evidence suggests that EOC spheroids in ascites are composed of not only cancer cells, but also non-malignant cells existing with higher abundance than EOC cells in ascites, including macrophages, mesothelial cells, and lymphocytes. Moreover, hetero-cellular spheroids are demonstrated to form more aggregated spheroids and have higher adhesion ability for the mesothelial layer. To improve the poor prognosis, we need to elucidate the mechanisms of spheroid formation and interactions with non-malignant cells in ascites that are a unique tumor microenvironment for EOC.
Phenotype of Mrps5-Associated Phylogenetic Polymorphisms Is Intimately Linked to Mitoribosomal Misreading
Authors: Juskeviciene Reda , Fritz Ann-Kristina , Brilkova Margarita , Akbergenov Rashid , Schmitt Karen , Rehrauer Hubert , Laczko Endre , Isnard-Petit Patricia , Thiam Kader , Eckert Anne , Schacht Jochen , Wolfer David P. , Böttger Erik C. , Shcherbakov Dimitri
Published: April 2022

We have recently identified point mutation V336Y in mitoribosomal protein Mrps5 (uS5m) as a mitoribosomal ram (ribosomal ambiguity) mutation conferring error-prone mitochondrial protein synthesis. In vivo in transgenic knock-in animals, homologous mutation V338Y was associated with a discrete phenotype including impaired mitochondrial function, anxiety-related behavioral alterations, enhanced susceptibility to noise-induced hearing damage, and accelerated metabolic aging in muscle. To challenge the postulated link between Mrps5 V338Y-mediated misreading and the in vivo phenotype, we introduced mutation G315R into the mouse Mrps5 gene as Mrps5 G315R is homologous to the established bacterial ram mutation RpsE (uS5) G104R. However, in contrast to bacterial translation, the homologous G → R mutation in mitoribosomal Mrps5 did not affect the accuracy of mitochondrial protein synthesis. Importantly, in the absence of mitochondrial misreading, homozygous mutant MrpS5G315R/G315R mice did not show a phenotype distinct from wild-type animals.
A Flower-like In2O3 Catalyst Derived via Metal–Organic Frameworks for Photocatalytic Applications
Authors: Munisamy Maniyazagan , Yang Hyeon-Woo , Perumal Naveenkumar , Kang Nayoung , Kang Woo Seung , Kim Sun-Jae
Published: April 2022

The most pressing concerns in environmental remediation are the design and development of catalysts with benign, low-cost, and efficient photocatalytic activity. The present study effectively generated a flower-like indium oxide (In2O3-MF) catalyst employing a convenient MOF-based solvothermal self-assembly technique. The In2O3-MF photocatalyst exhibits a flower-like structure, according to morphology and structural analysis. The enhanced photocatalytic activity of the In2O3-MF catalyst for 4-nitrophenol (4-NP) and methylene blue (MB) is likely due to its unique 3D structure, which includes a large surface area (486.95 m2 g−1), a wide spectrum response, and the prevention of electron–hole recombination compared to In2O3-MR (indium oxide-micro rod) and In2O3-MD (indium oxide-micro disc). In the presence of NaBH4 and visible light, the catalytic performances of the In2O3-MF, In2O3-MR, and In2O3-MD catalysts for the reduction of 4-NP and MB degradation were investigated. Using In2O3-MF as a catalyst, we were able to achieve a 99.32 percent reduction of 4-NP in 20 min and 99.2 percent degradation of MB in 3 min. Interestingly, the conversion rates of catalytic 4-NP and MB were still larger than 95 and 96 percent after five consecutive cycles of catalytic tests, suggesting that the In2O3-MF catalyst has outstanding catalytic performance and a high reutilization rate.
New Cases of Hypochromic Microcytic Anemia Due to Mutations in the SLC11A2 Gene and Functional Characterization of the G75R Mutation
Authors: Romero-Cortadellas Lídia , Hernández Gonzalo , Ferrer-Cortès Xènia , Zalba-Jadraque Laura , Fuster José Luis , Bermúdez-Cortés Mar , Galera-Miñarro Ana María , Pérez-Montero Santiago , Tornador Cristian , Sánchez Mayka
Published: April 2022

Divalent metal-iron transporter 1 (DMT1) is a mammalian iron transporter encoded by the SLC11A2 gene. DMT1 has a vital role in iron homeostasis by mediating iron uptake in the intestine and kidneys and by recovering iron from recycling endosomes after transferrin endocytosis. Mutations in SLC11A2 cause an ultra-rare hypochromic microcytic anemia with iron overload (AHMIO1), which has been described in eight patients so far. Here, we report two novel cases of this disease. The first proband is homozygous for a new SLC11A2 splicing variant (c.762 + 35A > G), becoming the first ever patient reported with a SLC11A2 splicing mutation in homozygosity. Splicing studies performed in this work confirm its pathogenicity. The second proband harbors the previously reported DMT1 G75R mutation in homozygosis. Functional studies with the G75R mutation in HuTu 80 cells demonstrate that this mutation results in improper DMT1 accumulation in lysosomes, which correlates with a significant decrease in DMT1 levels in patient-derived lymphoblast cell lines (LCLs). We also suggest that recombinant erythropoietin would be an adequate therapeutic approach for AHMIO1 patients as it improves their anemic state and may possibly contribute to mobilizing excessive hepatic iron.
Synaptic Plasticity Dysfunctions in the Pathophysiology of 22q11 Deletion Syndrome: Is There a Role for Astrocytes?
Authors: de Oliveira Figueiredo Eva , Bondiolotti Bianca , Laugeray Anthony , Bezzi Paola
Published: April 2022

The 22q11 deletion syndrome (DS) is the most common microdeletion syndrome in humans and gives a high probability of developing psychiatric disorders. Synaptic and neuronal malfunctions appear to be at the core of the symptoms presented by patients. In fact, it has long been suggested that the behavioural and cognitive impairments observed in 22q11DS are probably due to alterations in the mechanisms regulating synaptic function and plasticity. Often, synaptic changes are related to structural and functional changes observed in patients with cognitive dysfunctions, therefore suggesting that synaptic plasticity has a crucial role in the pathophysiology of the syndrome. Most interestingly, among the genes deleted in 22q11DS, six encode for mitochondrial proteins that, in mouse models, are highly expressed just after birth, when active synaptogenesis occurs, therefore indicating that mitochondrial processes are strictly related to synapse formation and maintenance of a correct synaptic signalling. Because correct synaptic functioning, not only requires correct neuronal function and metabolism, but also needs the active contribution of astrocytes, we summarize in this review recent studies showing the involvement of synaptic plasticity in the pathophysiology of 22q11DS and we discuss the relevance of mitochondria in these processes and the possible involvement of astrocytes.
Roles of Physicochemical and Structural Properties of RNA-Binding Proteins in Predicting the Activities of Trans-Acting Splicing Factors with Machine Learning
Authors: Zhu Lin , Li Wenjin
Published: April 2022

Trans-acting splicing factors play a pivotal role in modulating alternative splicing by specifically binding to cis-elements in pre-mRNAs. There are approximately 1500 RNA-binding proteins (RBPs) in the human genome, but the activities of these RBPs in alternative splicing are unknown. Since determining RBP activities through experimental methods is expensive and time consuming, the development of an efficient computational method for predicting the activities of RBPs in alternative splicing from their sequences is of great practical importance. Recently, a machine learning model for predicting the activities of splicing factors was built based on features of single and dual amino acid compositions. Here, we explored the role of physicochemical and structural properties in predicting their activities in alternative splicing using machine learning approaches and found that the prediction performance is significantly improved by including these properties. By combining the minimum redundancy–maximum relevance (mRMR) method and forward feature searching strategy, a promising feature subset with 24 features was obtained to predict the activities of RBPs. The feature subset consists of 16 dual amino acid compositions, 5 physicochemical features, and 3 structural features. The physicochemical and structural properties were as important as the sequence composition features for an accurate prediction of the activities of splicing factors. The hydrophobicity and distribution of coil are suggested to be the key physicochemical and structural features, respectively.
Serrated Colorectal Lesions: An Up-to-Date Review from Histological Pattern to Molecular Pathogenesis
Authors: Mezzapesa Martino , Losurdo Giuseppe , Celiberto Francesca , Rizzi Salvatore , d’Amati Antonio , Piscitelli Domenico , Ierardi Enzo , Di Leo Alfredo
Published: April 2022

Until 2010, colorectal serrated lesions were generally considered as harmless lesions and reported as hyperplastic polyps (HPs) by pathologists and gastroenterologists. However, recent evidence showed that they may bear the potential to develop into colorectal carcinoma (CRC). Therefore, the World Health Organization (WHO) classification has identified four categories of serrated lesions: hyperplastic polyps (HPs), sessile serrated lesions (SSLs), traditional serrated adenoma (TSAs) and unclassified serrated adenomas. SSLs with dysplasia and TSAs are the most common precursors of CRC. CRCs arising from serrated lesions originate via two different molecular pathways, namely sporadic microsatellite instability (MSI) and the CpG island methylator phenotype (CIMP), the latter being considered as the major mechanism that drives the serrated pathway towards CRC. Unlike CRCs arising through the adenoma–carcinoma pathway, APC-inactivating mutations are rarely shown in the serrated neoplasia pathway.
Recent Experimental Studies of Maternal Obesity, Diabetes during Pregnancy and the Developmental Origins of Cardiovascular Disease
Authors: Kereliuk Stephanie M. , Dolinsky Vernon W.
Published: April 2022

Globally, cardiovascular disease remains the leading cause of death. Most concerning is the rise in cardiovascular risk factors including obesity, diabetes and hypertension among youth, which increases the likelihood of the development of earlier and more severe cardiovascular disease. While lifestyle factors are involved in these trends, an increasing body of evidence implicates environmental exposures in early life on health outcomes in adulthood. Maternal obesity and diabetes during pregnancy, which have increased dramatically in recent years, also have profound effects on fetal growth and development. Mounting evidence is emerging that maternal obesity and diabetes during pregnancy have lifelong effects on cardiovascular risk factors and heart disease development. However, the mechanisms responsible for these observations are unknown. In this review, we summarize the findings of recent experimental studies, showing that maternal obesity and diabetes during pregnancy affect energy metabolism and heart disease development in the offspring, with a focus on the mechanisms involved. We also evaluate early proof-of-concept studies for interventions that could mitigate maternal obesity and gestational diabetes-induced cardiovascular disease risk in the offspring.
Adopting AI in the Context of Knowledge Work: Empirical Insights from German Organizations
Authors: von Richthofen Georg , Ogolla Shirley , Send Hendrik
Published: April 2022

Artificial Intelligence (AI) is increasingly adopted by organizations. In general, scholars agree that the adoption of AI will be associated with substantial changes in the workplace. Empirical evidence on the phenomenon remains scarce, however. In this article, we explore the adoption of AI in the context of knowledge work. Drawing on case study research in eight German organizations that have either implemented AI or are in the process of developing AI systems, we identify three pervasive changes that knowledge workers perceive: a shift from manual labor and repetitive tasks to tasks that involve reasoning and empathy, an emergence of new tasks and roles, and an emergence of new skill requirements. In addition, we identify three factors that are conducive to the development of AI systems in the context of knowledge work: leadership support, participative change management, and effective integration of domain knowledge. Theoretical and managerial implications are discussed.
User Pairing and Power Allocation for NOMA-CoMP Based on Rate Prediction
Authors: Wu Jiang , Sun Leyang , Jia Yubo
Published: April 2022

In this paper, we consider a non-orthogonal multiple access (NOMA) system with coordinated multi-point (CoMP), which is used in 5G cellular networks to guarantee the rate requirements from the different edge users. Based on the China Family Panel Studies (CFPS) dataset, we use several learning algorithms to predict users’ rate requirements according to their profiles. We propose a many-to-many two-side subchannel–user matching strategy, which can classify users into cell-center users, high-rate requirement edge users, and low-rate requirement edge users based on their status and learning prediction results, and pair users with different subchannels to form joint transmission CoMP (JT-CoMP) subchannels and dynamic point selection CoMP (DPS-CoMP) subchannels. Furthermore, a discrete power allocation algorithm based on group search is proposed. Simulation results show that our proposed algorithm outperforms the traditional NOMA-CoMP algorithm and maximum throughput (MT) NOMA-CoMP algorithm. It maximizes the rate of high-rate requirement edge users while guaranteeing user fairness.
Surveys of Drosophila suzukii (Diptera: Drosophilidae) and Its Host Fruits and Associated Parasitoids in Northeastern China
Authors: Wang Jue , Zheng Yanan , Fan Lichun , Wang Weitao
Published: April 2022

Spotted-wing drosophila, Drosophila suzukii (Matsumura), is a worldwide quarantine pest that is currently undergoing a rapid range expansion in the Americas, Europe, and parts of Africa. It feeds and breeds on soft-skinned fruits such as raspberries, blueberries, and cherries, and can cause significant economic losses to fruit production. This study investigated the occurrence of D. suzukii and its wild host fruits and parasitoids in Liaoning, Northeast China for the first time. Sentinel traps were used to monitor D. suzukii adults, and suspected fruits were collected weekly in four different locations (Wafangdian, Faku, Fengcheng, and Shenyang). The results showed that D. suzukii were distributed in the sweet soft-skinned fruit-production areas of Liaoning, and raspberry was the most infested fruit. During the field survey, four species of wild berries from non-crop habitats were found infested by D. suzukii, and two species of parasitoids (Leptopilina japonica and Asobara japonica) were collected. D. suzukii adult-population dynamics throughout the survey period (June to October) were similar in different survey locations; adult fly populations increased and peaked in August, and then declined until the fly was no longer detectable in October.
Transcriptomic and Metabolomic Responses in Cotton Plant to Apolygus lucorum Infestation
Authors: Chen Han , Su Honghua , Zhang Shuai , Jing Tianxing , Liu Zhe , Yang Yizhong
Published: April 2022

With the wide-scale adoption of transgenic Bacillus thuringiensis (Bt) cotton, Apolygus lucorum (Meyer-Dür) has become the most serious pest and has caused extensive yield loss in cotton production. However, little is known about the defense responses of cotton at the seedling stage to A. lucorum feeding. In this study, to elucidate the cotton defense mechanism, cotton leaves were damaged by A. lucorum for 0, 4, 12 and 24 h. The transcriptomic results showed that A. lucorum feeding elicits a rapid and strong defense response in gene expression during the whole infestation process in cotton plants. Further analysis revealed that at each assessment time, more differentially expressed genes were up-regulated than down-regulated. The integrated analysis of transcriptomic and metabolic data showed that most of the genes involved in jasmonic acid (JA) biosynthesis were initially up-regulated, and this trend continued during an infestation. Meanwhile, the content levels of JA and its intermediate products were also significantly increased throughout the whole infestation process. The similar trend was displayed in condensed tannins biosynthesis. This research proved that, after plants are damaged by A. lucorum, the JA pathway mediates the defense mechanisms in cotton plants by promoting the accumulation of condensed tannins as a defense mechanism against A. lucorum. These results will help us to discover unknown defensive genes and improve the integrated pest management of A. lucorum.
The Early Season Community of Flower-Visiting Arthropods in a High-Altitude Alpine Environment
Authors: Bonelli Marco , Eustacchio Elena , Avesani Daniele , Michelsen Verner , Falaschi Mattia , Caccianiga Marco , Gobbi Mauro , Casartelli Morena
Published: April 2022

In mountain ecosystems, climate change can cause spatiotemporal shifts, impacting the composition of communities and altering fundamental biotic interactions, such as those involving flower-visiting arthropods. On of the main problems in assessing the effects of climate change on arthropods in these environments is the lack of baseline data. In particular, the arthropod communities on early flowering high-altitude plants are poorly investigated, although the early season is a critical moment for possible mismatches. In this study, we characterised the flower-visiting arthropod community on the early flowering high-altitude Alpine plant, Androsace brevis (Primulaceae). In addition, we tested the effect of abiotic factors (temperature and wind speed) and other variables (time, i.e., hour of the day, and number of flowers per plant) on the occurrence, abundance, and diversity of this community. A. brevis is a vulnerable endemic species growing in the Central Alps above 2000 m asl and flowering for a very short period immediately after snowmelt, thus representing a possible focal plant for arthropods in this particular moment of the season. Diptera and Hymenoptera were the main flower visitors, and three major features of the community emerged: an evident predominance of anthomyiid flies among Diptera, a rare presence of bees, and a relevant share of parasitoid wasps. Temperature and time (hour of the day), but not wind speed and number of flowers per plant, affected the flower visitors’ activity. Our study contributes to (1) defining the composition of high-altitude Alpine flower-visiting arthropod communities in the early season, (2) establishing how these communities are affected by environmental variables, and (3) setting the stage for future evaluation of climate change effects on flower-visiting arthropods in high-altitude environments in the early season.
The Risk Factors for Progression to Chronic Pancreatitis in Patients with Past-History of Acute Pancreatitis: A Retrospective Analysis Based on Mechanistic Definition
Authors: Yamamiya Akira , Tominaga Keiichi , Hoshi Koki , Nagashima Kazunori , Minaguchi Takahito , Haruyama Yasuo , Irisawa Atsushi
Published: April 2022

Background: According to the mechanistic definition, the history of acute pancreatitis (AP) is a risk factor for chronic pancreatitis (CP). However, the etiology and severity of previous AP involved in the progression to CP have not been clarified. Here, we investigated risk factors for the progression to CP in patients with past-history of AP. Methods: Sixty-four patients with AP who were followed-up for at least two years at our institution between April 2009 and March 2017 were enrolled. The multivariate analysis was performed based on the risk factors extracted by univariate analysis. Results: Among the 64 patients, 13 patients (20.3%) progressed to CP (PCP group), while 48 did not (non-PCP group). Regarding the etiology of AP, rate of alcohol AP was significantly higher in the PCP group (76.9% vs. 33.3%, p = 0.003). In univariate analysis, smoking, number of previous AP, and alcohol consumption and drinking habits (Alcohol Use Disorders Identification Test-Concise; AUDIT-C) were identified as factors associated with progression to CP. Furthermore, multivariate analysis showed that AUDIT-C ≥ 6 points (male) and 4 points (female) after AP was a significant risk factor for CP (p = 0.003). Conclusions: Our results indicated that AUDIT-C ≥ 6 points (male) and 4 points (female) after AP was a risk factor in the process of progression to CP in patients with past-history of AP.
Impact of the COVID-19 Pandemic on Maternal Well-Being during Pregnancy
Authors: Pascal Rosalia , Crovetto Francesca , Casas Irene , Youssef Lina , Trilla Cristina , Larroya Marta , Cahuana Alex , Boada David , Foraster Maria , Llurba Elisa , Sunyer Jordi , Crispi Fàtima , Gratacos Eduard , Gómez-Roig María Dolores
Published: April 2022

The outbreak of a pandemic has negative psychological effects. We aimed to determine the impact of the SARS-CoV-2 pandemic during pregnancy and identify the risk factors for maternal well-being. A multicenter, prospective, population-based study was carried out that included women (n = 1320) who were pregnant during the SARS-CoV-2 pandemic in Barcelona (Spain) compared against a pre-pandemic cohort (n = 345). Maternal well-being was assessed using the validated World Health Organization Well-Being Index Questionnaire (WHO-5 Index). Pregnant women attended during the COVID-19 pandemic showed worst WHO-5 well-being scores (median (IQR) of 56 (36–72) for the pandemic cohort vs. 64 (52–76) for the pre-pandemic cohort p < 0.001), with 42.8% of women presenting a poor well-being score vs. 28% for the pre-pandemic cohort (p < 0.001). Presence of a previous psychiatric disorder (OR 7.1; 95% CI 2.6–19, p < 0.001), being in the third trimester of pregnancy (OR 1.7; 95% CI 1.5–2, p < 0.001), or requiring hospital admission for COVID-19 (OR 4.7; 95% CI 1.4–16.7, p = 0.014), significantly contributed to low maternal well-being during the COVID-19 pandemic (multivariate analysis). Being infected by SARS-CoV-2 was not associated with a lower well-being score. We conclude that, during the COVID-19 pandemic, there were higher rates of poor maternal well-being; the infection of SARS-CoV-2 itself did not worsen maternal well-being, but other factors as psychiatric disorders, being in the third trimester of pregnancy or hospital admission for COVID-19 disease did.
Strategy and Challenges of Paraclinical Examinations in Adult-Onset Still’s Disease
Authors: Poursac Nicolas , Odriozola Itsaso , Truchetet Marie-Elise
Published: April 2022

Adult-onset Still’s disease is a complex autoinflammatory disease with a multifactorial etiology. Its presentation is less stereotypical than that of a monogenic autoinflammatory disease and is actually relatively common with few specific signs. To avoid under- or over-prescription of complementary examinations, it is useful to advance in a structured manner, taking into consideration the actual added value of each supplemental examination. In this review, we detail the different complementary tests used in adult Still’s disease. We consider them from three different angles: positive diagnostic approach, the differential diagnosis, and the screening for complications of the disease. After discussing the various tests at our disposal, we look at the classical diagnostic strategy in order to propose a structured algorithm that can be used in clinical practice. We conclude with the prospects of new complementary examinations, which could in the future modify the management of patients.
E-Cadherin Signaling in Salivary Gland Development and Autoimmunity
Authors: Sisto Margherita , Ribatti Domenico , Lisi Sabrina
Published: April 2022

E-cadherin, the major epithelial cadherin, is located in regions of cell–cell contact known as adherens junctions. E-cadherin contributes to the maintenance of the epithelial integrity through homophylic interaction; the cytoplasmic tail of E-cadherin directly binds catenins, forming a dynamic complex that regulates several intracellular signal transduction pathways, including epithelial-to-mesenchymal transition (EMT). Recent progress uncovered a novel and critical role for this adhesion molecule in salivary gland (SG) development and in SG diseases. We summarize the structure and regulation of the E-cadherin gene and transcript in view of the role of this remarkable protein in SG morphogenesis, focusing, in the second part of the review, on altered E-cadherin expression in EMT-mediated SG autoimmunity.
The Treatment of Heart Failure in Patients with Chronic Kidney Disease: Doubts and New Developments from the Last ESC Guidelines
Authors: Beltrami Matteo , Milli Massimo , Dei Lorenzo Lupo , Palazzuoli Alberto
Published: April 2022

Patients with heart failure (HF) and associated chronic kidney disease (CKD) are a population less represented in clinical trials; additionally, subjects with more severe estimated glomerular filtration rate reduction are often excluded from large studies. In this setting, most of the data come from post hoc analyses and retrospective studies. Accordingly, in patients with advanced CKD, there are no specific studies evaluating the long-term effects of the traditional drugs commonly administered in HF. Current concerns may affect the practical approach to the traditional treatment, and in this setting, physicians are often reluctant to administer and titrate some agents acting on the renin angiotensin aldosterone system and the sympathetic activity. Therefore, the extensive application in different HF subtypes with wide associated conditions and different renal dysfunction etiologies remains a subject of debate. The role of novel drugs, such as angiotensin receptor blocker neprilysin inhibitors and sodium glucose linked transporters 2 inhibitors seems to offer a new perspective in patients with CKD. Due to its protective vascular and hormonal actions, the use of these agents may be safely extended to patients with renal dysfunction in the long term. In this review, we discussed the largest trials reporting data on subjects with HF and associated CKD, while suggesting a practical stepwise algorithm to avoid renal and cardiac complications.
Vertebral Osteomyelitis and Infective Endocarditis Co-Infection
Authors: Morimoto Tadatsugu , Hirata Hirohito , Otani Koji , Nakamura Eiichiro , Miyakoshi Naohisa , Terashima Yoshinori , Wada Kanichiro , Kobayashi Takaomi , Murayama Masatoshi , Tsukamoto Masatsugu , Mawatari Masaaki
Published: April 2022

Many cases of vertebral osteomyelitis (VO) and infective endocarditis (IE) co-infection have been reported, and it has been recognized that attention should be paid to the possibility of both diseases co-existing during diagnosis and treatment. However, the incidence, clinical status, and outcomes of IE in patients with VO remain unclear. For this study, the eligibility criteria for patient recruitment included all cases of VO at the five medical university hospitals. Patients with a history of spinal surgery were excluded from this study. Echocardiography was routinely performed for all patients with VO. IE was diagnosed according to the modified Duke criteria for definite endocarditis. We analyzed demographic data, underlying conditions, clinical features, laboratory data, echocardiography, radiologic images, treatments, and outcomes. VO was diagnosed in 59 patients and IE was diagnosed in seven patients (12%). There were no significant differences in the clinical features, microorganisms, or radiographic status between the VO-IE co-infection and VO-only groups. In this study, using routine echocardiography for VO, the IE prevalence was 12%. The lack of specific clinical features and laboratory findings may hamper the diagnosis of IE. Therefore, clinicians are always required to suspect IE in patients with VO.
Reply to Kalfert, D. Comment on “Zwierz et al. The Long-Term Effects of 12-Week Intranasal Steroid Therapy on Adenoid Size, Its Mucus Coverage and Otitis Media with Effusion: A Cohort Study in Preschool Children. J. Clin. Med. 2022, 11, 507”
Authors: Zwierz Aleksander
Published: April 2022

We wish to thank the author for raising the issues of how we performed the adenoid size classification and why we did not classify the condition of the nasopharyngeal orifice of the Eustachian tube [...]
The Implant Proteome—The Right Surgical Glue to Fix Titanium Implants In Situ
Authors: Jäger Marcus , Latosinska Agnieszka , Herten Monika , Busch André , Grupp Thomas , Sowislok Andrea
Published: April 2022

Titanium implants are frequently applied to the bone in orthopedic and trauma surgery. Although these biomaterials are characterized by excellent implant survivorship and clinical outcomes, there are almost no data available on the initial protein layer binding to the implant surface in situ. This study aims to investigate the composition of the initial protein layer on endoprosthetic surfaces as a key initiating step in osseointegration. In patients qualified for total hip arthroplasty, the implants are inserted into the femoral canal, fixed and subsequently explanted after 2 and 5 min. The proteins adsorbed to the surface (the implant proteome) are analyzed by liquid chromatography–tandem mass spectrometry (LC-MS/MS). A statistical analysis of the proteins’ alteration with longer incubation times reveals a slight change in their abundance according to the Vroman effect. The pathways involved in the extracellular matrix organization of bone, sterile inflammation and the beginning of an immunogenic response governed by neutrophils are significantly enriched based on the analysis of the implant proteome. Those are generally not changed with longer incubation times. In summary, proteins relevant for osseointegration are already adsorbed within 2 min in situ. A deeper understanding of the in situ protein–implant interactions in patients may contribute to optimizing implant surfaces in orthopedic and trauma surgery.