bluuebunny/crossref_metadata_2025 huggingface dataset:
Controlling and Limiting Infection Risk, Thermal Discomfort, and Low Indoor Air Quality in a Classroom through Natural Ventilation Controlled by Smart Windows
Authors: Grygierek Krzysztof , Nateghi Seyedkeivan , Ferdyn-Grygierek Joanna , Kaczmarczyk Jan
Published: January 2023

In this study, a controller method for window opening was developed to naturally ventilate a classroom with 30 occupants. The aim was to improve indoor environment quality and limit the probability of COVID infection risk simultaneously. The study was based on a building performance simulation using combined EnergyPlus, CONTAM, and Python programs. Seven cases with automatically opening windows were considered. Opening window parameters were optimized by genetic algorithms. It was shown that the optimized controller with indoor environment functions improved classroom ventilation and considerably decreased CO2 concentration compared to a reference case where the windows were opened only during breaks, and the controller also improved occupants’ thermal comfort. However, there was a noticeable increase in energy demand, caused by the increased air change rate. Introducing the probability of infection risk function to the controller did not reduce the transmission risk substantially, and the probability of infection transmission was high for 80% of the classroom occupancy time. The risk of infection changed only when additional actions were taken, such as introducing face masks, indoor air cleaners, or reducing the number of students present in the classroom. In these cases, it was possible to prevent the infection transmission for more than 90% of the lecture time (R0 < 1).
Promising Bioalcohols for Low-Emission Vehicles
Authors: Tanwar Manju Dhakad , Torres Felipe Andrade , Alqahtani Ali Mubarak , Tanwar Pankaj Kumar , Bhand Yashas , Doustdar Omid
Published: January 2023

In recent decades, many kinds of research have been conducted on alternative fuels for compression ignition (CI) engines. Low/zero-carbon fuels, such as bioalcohols and hydrogen, are the most promising alternative fuels and are extensively studied because of their availability, ease of manufacturing, and environmental benefits. Using these promising fuels in CI engines is environmentally and economically beneficial. The most common alcohols are methanol, ethanol, isopropanol, propanol, butanol, n-butanol, tert-butanol, iso-butanol, and pentanol. The primary objective of this review paper is to examine the impact of bioalcohols and their blends with conventional diesel fuel in CI engines since these fuels possess characteristic properties that impact overall engine performance and exhaust emissions. This research also indicated that alcohols and blended fuels could be used as fuels in compression ignition engines. Chemical and physical properties of alcohols were examined, such as lubricity, viscosity, calorific value, and cetane number, and their combustion characteristics in compression ignition engines provide a comprehensive review of their potential biofuels as alternative fuels.
Deep Level Transient Fourier Spectroscopy Investigation of Electron Traps on AlGaN/GaN-on-Si Power Diodes
Authors: Rigaud-Minet Florian , Raynaud Christophe , Buckley Julien , Charles Matthew , Pimenta-Barros Patricia , Gwoziecki Romain , Gillot Charlotte , Sousa Véronique , Morel Hervé , Planson Dominique
Published: January 2023

Many kinds of defects are present in AlGaN/GaN-on-Si based power electronics devices. Their identification is the first step to understand and improve device performance. Electron traps are investigated in AlGaN/GaN-on-Si power diodes using deep level transient Fourier spectroscopy (DLTFS) at different bias conditions for two Schottky contact’s etching recipes. This study reveals seven different traps corresponding to point defects. Their energy level ET ranged from 0.4 eV to 0.57 eV below the conduction band. Among them, two new traps are reported and are etching-related: D3 (ET = 0.47–0.48 eV; σ ≈ 10−15 cm2) and D7 (ET = 0.57 eV; σ = 4.45 × 10−12 cm2). The possible origin of the other traps are discussed with respect to the GaN literature. They are proposed to be related to carbon and nitrogen vacancies or to carbon, such as CN-CGa. Some others are likely due to crystal surface recombination, native defects or a related complex, or to the nitrogen antisite: NGa.
Wing Deformation of an Airborne Wind Energy System in Crosswind Flight Using High-Fidelity Fluid–Structure Interaction
Authors: Pynaert Niels , Haas Thomas , Wauters Jolan , Crevecoeur Guillaume , Degroote Joris
Published: January 2023

Airborne wind energy (AWE) is an emerging technology for the conversion of wind energy into electricity. There are many types of AWE systems, and one of them flies crosswind patterns with a tethered aircraft connected to a generator. The objective is to gain a proper understanding of the unsteady interaction of air and this flexible and dynamic system during operation, which is key to developing viable, large AWE systems. In this work, the effect of wing deformation on an AWE system performing a crosswind flight maneuver was assessed using high-fidelity time-varying fluid–structure interaction simulations. This was performed using a partitioned and explicit approach. A computational structural mechanics (CSM) model of the wing structure was coupled with a computational fluid dynamics (CFD) model of the wing aerodynamics. The Chimera/overset technique combined with an arbitrary Lagrangian–Eulerian (ALE) formulation for mesh deformation has been proven to be a robust approach to simulating the motion and deformation of an airborne wind energy system in CFD simulations. The main finding is that wing deformation in crosswind flights increases the symmetry of the spanwise loading. This property could be used in future designs to increase the efficiency of airborne wind energy systems.
Lactic Bacteria in Artisanal Cheese: Characterization through Metagenomics
Authors: Erhardt Magnolia Martins , Oliveira Wemerson de Castro , Fröder Hans , Marques Pedro Henrique , Oliveira Maria Beatriz Prior Pinto , Richards Neila Silvia Pereira dos Santos
Published: January 2023

Artisanal cheese, produced with raw milk by a predominantly manual approach, has a historical and cultural tradition related to the region of origin. Given its economic and cultural importance, the main objective of this study was to investigate and characterize the diversity of lactic acid bacteria (LAB) of artisanal cheeses produced and traded by family agro-industries in a region of southern Brazil. The LAB composition of artisanal cheese samples, belonging to different municipalities of the Region of Vale do Taquari, were characterized by the next-generation sequencing (NGS) method, amplifying the V3/V4 region of the 16S rRNA gene. A total of 35 LAB species, distributed in seven genera, were identified, and rarefaction analysis suggested that the total diversity assessed by 16S rRNA analysis was high in the analyzed samples. The average Ph ranged from 4.6 to 6.6, and a correlation with the genus Lactococcus (r = 0.62) was the most expressive. The LAB genera identified in the cheese samples were Bavariicococcus, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Marinillactibacillus, and Pediococcus. Lactococcus lactis was the most predominant species, present in all samples. Although some species have been identified in the three altitudes studied, the abundance varied according to geographic environments. Enterococcus italicus is more present at high altitudes, unlike Lactococcus plantarum and Lactococcus raffinolactis at low altitudes. Lactococcus lactis was present in the three geographic environments evaluated, but the highest abundance was observed at high altitudes. The identification of LAB present in fermented cheeses is essential to understand the organoleptic quality during the maturation process as well as to establish the shelf life, including the safety and the overall quality of the cheese. This specific microbiota contributes to the flavor and unique characteristics of the regional dairy products, and on the other hand can be a source of specific starter cultures that guarantee the product’s identity.
A V2V Identity Authentication and Key Agreement Scheme Based on Identity-Based Cryptograph
Authors: Li Qiang
Published: January 2023

Cellular vehicle to everything (C-V2X) is a technology to achieve vehicle networking, which can improve traffic efficiency and traffic safety. As a special network, the C-V2X system faces many security risks. The vehicle to vehicle (V2V) communication transmits traffic condition data, driving path data, user driving habits data, and so on. It is necessary to ensure the opposite equipment is registered C-V2X equipment (installed in the vehicle), and the data transmitted between the equipment is secure. This paper proposes a V2V identity authentication and key agreement scheme based on identity-based cryptograph (IBC). The C-V2X equipment use its vehicle identification (VID) as its public key. The key management center (KMC) generates a private key for the C-V2X equipment according to its VID. The C-V2X equipment transmit secret data encrypted with the opposite equipment public key to the other equipment, they authenticate each other through a challenge response protocol based on identity-based cryptography, and they negotiate the working key used to encrypt the communication data. The scheme can secure the V2V communication with low computational cost and simple architecture and meet the lightweight and efficient communication requirements of the C-V2X system.
Otoliths and Their Applications in Fishery Science
Authors: Ferri Josipa
Published: January 2023

Otoliths are one of the most useful and important biological structures for the various studies of fish, leading to many practical applications that are not limited only to ichthyology [...]
TRPV1 Receptor Identification in Rainbow Trout (Oncorhynchus mykiss) and Evaluation of the Effects Produced by Ocimum basilicum Super Critical Fluid Extract
Authors: Vercelli Cristina , Amadori Michela , Tursi Massimiliano , Gambino Graziana , Pastorino Paolo , Prearo Marino , Ala Ugo , Barbero Raffaella , Re Giovanni
Published: January 2023

Transient receptor potential vanilloid type 1 (TRPV1) has been investigated in humans and mammals; in recent years, some researchers have focused on this receptor in fishes. The present study aimed to identify TRPV1 receptors in cultures of RT-gill W1 cells and in the organs of rainbow trout (Oncorhynchus mykiss), in addition to evaluating the possible modulation induced by super critical fluid extract of basil (Ocimum basilicum), named F1-BEO. In vitro evaluation consisted of cell cultures and immunocytochemistry assays. During in vivo experimental sessions, eighty trout were divided into five groups that received a fish diet supplemented with 0, 0.5, 1, 2 and 3% w/w F1-BEO. Forty trout were euthanized after 15 and 30 days; organs were collected and processed according to the immunohistochemistry technique. Receptor expression was quantitatively measured using Image Pro Plus software. TRPV1 was identified in RT-gill W1 cells and in all organs, with a higher positivity in the muscle layers of the stomach, intestine and kidneys. F1-BEO induced an increased expression of TRPV1 in the stomach while a lower expression was appreciated in the bowel. No morphological alterations have been highlighted in the liver or kidneys. Further investigation will be necessary to evaluate the functionality of this receptor in rainbow trout.
Changing the IgE Binding Capacity of Tropomyosin in Shrimp through Structural Modification Induced by Cold Plasma and Glycation Treatment
Authors: Wang Feng-Qi , Cheng Jun-Hu , Keener Kevin
Published: January 2023

Tropomyosin (TM) is the major allergen of shrimp (Penaeus chinensis). Previous studies showed that separate cold plasma or glycation have their drawback in reducing allergenicity of TM, including effectiveness and reliability. In the current study, a new processing combining cold plasma (CP) and glycation was proposed and its effect on changing IgE binding capacity of TM from shrimp was investigated. Obtained results showed the IgE binding capacity of TM was reduced by up to 40% after CP (dielectric barrier discharge, 60 kV, 1.0 A) combined with glycation treatment (4 h, 80 °C), compared with the less than 5% reduction after single CP or glycation treatment. Notably, in contrast to the general way of CP prompting glycation, this study devised a new mode of glycation with ribose after CP pretreatment. The structural changes of TM were explored to explain the decreased IgE binding reactivity. The results of multi-spectroscopies showed that the secondary and tertiary structures of TM were further destroyed after combined treatment, including the transformation of 50% α-helix to β-sheet and random coils, the modification and exposure of aromatic amino acids, and the increase of surface hydrophobicity. The morphology analysis using atomic force microscope revealed that the combined processing made the distribution of TM particles tend to disperse circularly, while it would aggregate after either processing treatment alone. These findings confirmed the unfolding and reaggregation of TM during combined processing treatment, which may result in the remarkable reduction of IgE binding ability. Therefore, the processing of CP pretreatment combined with glycation has the potential to reduce or even eliminate the allergenicity of seafood.
Characteristics and Correlation of the Microbial Communities and Flavor Compounds during the First Three Rounds of Fermentation in Chinese Sauce-Flavor Baijiu
Authors: Xu Youqiang , Wu Mengqin , Niu Jialiang , Lin Mengwei , Zhu Hua , Wang Kun , Li Xiuting , Sun Baoguo
Published: January 2023

Sauce-flavor Baijiu is representative of solid-state fermented Baijiu. It is significant to deeply reveal the dynamic changes of microorganisms in the manufacturing process and their impact on the formation of flavor chemicals correlated with the quality of Baijiu. Sauce-flavor Baijiu manufacturing process can be divided into seven rounds, from which seven kinds of base Baijius are produced. The quality of base Baijiu in the third round is significantly better than that in the first and second rounds, but the mystery behind the phenomenon has not yet been revealed. Based on high-throughput sequencing and flavor analysis of fermented grains, and correlation analysis, the concentrations of flavor chemicals in the third round of fermented grains were enhanced, including esters hexanoic acid, ethyl ester; octanoic acid, ethyl ester; decanoic acid, ethyl ester; dodecanoic acid, ethyl ester; phenylacetic acid, ethyl ester; 3-(methylthio)-propionic acid ethyl ester; acetic acid, phenylethyl ester; hexanoic acid, butyl ester, and other flavor chemicals closely related to the flavor of sauce-flavor Baijiu, such as tetramethylpyrazine. The changes in flavor chemicals should be an important reason for the quality improvement of the third round of base Baijiu. Correlation analysis showed that ester synthesis was promoted by the bacteria genus Lactobacillus and many low abundances of fungal genera, and these low abundances of fungal genera also had important contributions to the production of tetramethylpyrazine. Meanwhile, the degrading metabolic pathway of tetramethylpyrazine was investigated, and the possible microorganisms were correlated. These results clarified the base Baijiu quality improvement of the third round and helped to provide a theoretical basis for improving base Baijiu quality.
Helium Atmospheric Pressure Plasma Jet Effects on Two Cultivars of Triticum aestivum L.
Authors: Burducea Ion , Burducea Cristina , Mereuta Paul-Emil , Sirbu Stefan-Robert , Iancu Decebal-Alexandru , Istrati Melania-Beatrice , Straticiuc Mihai , Lungoci Constantin , Stoleru Vasile , Teliban Gabriel-Ciprian , Robu Teodor , Burducea Marian , Nastuta Andrei Vasile
Published: January 2023

The use of cold plasma in the treatment of seeds before sowing presents a promising technique for sustainable agriculture. The objective of this study is to evaluate the effect of cold plasma treatment on the morphology of wheat seeds (Triticum aestivum L. ‘Dacic’ and ‘Otilia’), their germination, biochemical composition, and the nutritional quality of wheat grass. Wheat seeds were morphologically and elementally characterized by atomic force microscopy (AFM), scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDX), X-ray computer tomography (CT), and particle-induced X-ray emission (PIXE). Helium was used as a working gas for plasma generation and the analysis of the species produced showed the presence of NOγ, OH, N2 and N2+ and O. Evaluation of germination and plant growth for 10 days (wheat grass stage) highlighted a specific trend for each cultivar. The biochemical analysis of wheat grass highlighted an increase in the chlorophyll content in the plasma-treated variants, an increase in the flavonoid and polyphenol content in ‘Dacic’-treated variant, while the soluble protein content, antioxidant activity, and color were not affected. The analysis of the nutritional quality of wheat grass by the FT-NIR analytical technique highlighted an increase in the ash content in the plasma-treated wheat cultivars, while the humidity, proteins, neutral detergent fiber (NDF), acid detergent fiber (ADF), and energy values were not affected.
Feature Reduction for the Classification of Bruise Damage to Apple Fruit Using a Contactless FT-NIR Spectroscopy with Machine Learning
Authors: Nturambirwe Jean Frederic Isingizwe , Hussein Eslam A. , Vaccari Mattia , Thron Christopher , Perold Willem Jacobus , Opara Umezuruike Linus
Published: January 2023

Spectroscopy data are useful for modelling biological systems such as predicting quality parameters of horticultural products. However, using the wide spectrum of wavelengths is not practical in a production setting. Such data are of high dimensional nature and they tend to result in complex models that are not easily understood. Furthermore, collinearity between different wavelengths dictates that some of the data variables are redundant and may even contribute noise. The use of variable selection methods is one efficient way to obtain an optimal model, andthis was the aim of this work. Taking advantage of a non-contact spectrometer, near infrared spectral data in the range of 800–2500 nm were used to classify bruise damage in three apple cultivars, namely ‘Golden Delicious’, ‘Granny Smith’ and ‘Royal Gala’. Six prominent machine learning classification algorithms were employed, and two variable selection methods were used to determine the most relevant wavelengths for the problem of distinguishing between bruised and non-bruised fruit. The selected wavelengths clustered around 900 nm, 1300 nm, 1500 nm and 1900 nm. The best results were achieved using linear regression and support vector machine based on up to 40 wavelengths: these methods reached precision values in the range of 0.79–0.86, which were all comparable (within error bars) to a classifier based on the entire range of frequencies. The results also provided an open-source based framework that is useful towards the development of multi-spectral applications such as rapid grading of apples based on mechanical damage, and it can also be emulated and applied for other types of defects on fresh produce.
Effect of Bamboo Essential Oil on the Oxidative Stability, Microbial Attributes and Sensory Quality of Chicken Meatballs
Authors: Das Jyotishka Kumar , Chatterjee Niloy , Pal Srija , Nanda Pramod Kumar , Das Annada , Das Ligen , Dhar Pubali , Das Arun K.
Published: January 2023

This study explores the efficacy of bamboo essential oil (BEO) incorporated at 15 ppm (T1, BEO-I) and 30 ppm (T2, BEO-II) on the overall physicochemical and oxidative stability, microbial deterioration, and sensory acceptability of meatballs stored for 20 days under refrigerated conditions. Analysis of various parameters, including physicochemical quality, color (CIE L*, CIE a* and CIE b*), generation of oxidative products (TBARS), microbial growth, and sensory acceptability of meatballs were evaluated at 5-day intervals. In addition, the total phenolics and flavonoid content of BEO were estimated, and fatty acids were determined by Gas chromatography (GC.) To gain insights into the biological activities of the BEO, antioxidant assays were determined in vitro using various methods. The antibacterial activity of BEO was also evaluated against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Vibrio cholera, Salmonella Typhimurium, Shigella flexneri, Proteus vulgaris, Escherichia coli and Klebsiella pneumoniae) bacterial strains. The BEO contained a good quantity of total phenolics and flavonoids. In addition, the oil exhibited very potent antioxidant activity scavenging reactive oxygen and other such species, effectively showing IC50 at a very minimal concentration. Further, the BEO exhibited a strong antibacterial effect with MICs within 2 µL and MBCs from 5 to 7 µL for Gram-positive as well as Gram-negative bacteria, respectively. At both the concentrations used, BEO did not show any negative effect on the color of cooked meatballs but rather increased the microbiological and oxidative stability during the overall storage period. Meatballs treated with BEO had considerably reduced oxidative changes in terms of TBARS levels compared to the control. The total viable microbial count was lowest in BEO-treated meatballs and the highest in control. Both control and treated meatballs had a desirable flavor and good acceptability. The sensory attributes and aroma of treated meatballs were better and acceptable during the storage study, whereas the control samples were disliked by the panelists on 15th day. From this study, it can be concluded that bamboo essential oil could be used as a benign and non-toxic preservative to improve the quality and shelf life of cooked meatballs stored under refrigerated conditions.
Biochemical and Genomic Characterization of Two New Strains of Lacticaseibacillus paracasei Isolated from the Traditional Corn-Based Beverage of South Africa, Mahewu, and Their Comparison with Strains Isolated from Kefir Grains
Authors: Moiseenko Konstantin V. , Begunova Anna V. , Savinova Olga S. , Glazunova Olga A. , Rozhkova Irina V. , Fedorova Tatyana V.
Published: January 2023

Lacticaseibacillus paracasei (formerly Lactobacillus paracasei) is a nomadic lactic acid bacterium (LAB) that inhabits a wide variety of ecological niches, from fermented foodstuffs to host-associated microenvironments. Many of the isolated L. paracasei strains have been used as single-strain probiotics or as part of a symbiotic consortium within formulations. The present study contributes to the exploration of different strains of L. paracasei derived from non-conventional isolation sources—the South African traditional fermented drink mahewu (strains MA2 and MA3) and kefir grains (strains KF1 and ABK). The performed microbiological, biochemical and genomic comparative analyses of the studied strains demonstrated correlation between properties of the strains and their isolation source, which suggests the presence of at least partial strain adaptation to the isolation environments. Additionally, for the studied strains, antagonistic activities against common pathogens and against each other were observed, and the ability to release bioactive peptides with antioxidant and angiotensin I-converting enzyme inhibitory (ACE-I) properties during milk fermentation was investigated. The obtained results may be useful for a deeper understanding of the nomadic lifestyle of L. paracasei and for the development of new starter cultures and probiotic preparations based on this LAB in the future.
Evaluating the Food Profile in Qatar within the Energy–Water–Food Nexus Approach
Authors: Alrebei Odi Fawwaz , Al-Ansari Tareq , Al-Kuwari Mohammad S. , Amhamed Abdulkarem
Published: January 2023

Finding a balance between the capacity for production and the rising demand for food is the first step toward achieving food security. To achieve sustainable development on a national scale, decision-makers must use an energy, water, and food nexus approach that considers the relationships and interactions among these three resources as well as the synergies and trade-offs that result from the way they are handled. Therefore, this paper evaluates the Energy–Water–Food Nexus Profile of Qatar at a superstructural level by applying the Business-As-Usual (BAU) storyline; thus, trends of past data have been used to provide future projections to 2050 using the statistical prediction tools such as the compound annual growth rates of food demand (CAGRFD), international supply (CAGRFI), and the average local food supply change factor (c¯). Once the BAU storyline has been generated, the source-to-demand correlations have been defined for each food category. Such correlations include the annual and average ratios of the local food supply to the total demand (i.e., αi and α¯) and the ratios of the local food supply to the international supply (i.e., βi and β¯). In addition, as an effort to identify the required action to reach food self-sustainability, the additional local food supply to achieve (xi,add) and its ratio to the local demand (γ) have been defined. The highest average ratio of the local food supply to the total demand (αi) was found for the meat category, which was estimated to be 48.3%. Finally, to evaluate the feasibility of attaining food self-sustainability in Qatar, the water consumption (Vw,i) and its corresponding required energy for each food category have been estimated.
Topological Structure of Solution Sets of Fractional Control Delay Problem
Authors: Ghafli Ahmed A. Al , Shafqat Ramsha , Niazi Azmat Ullah Khan , Abuasbeh Kinda , Awadalla Muath
Published: January 2023

This paper is concerned with the existence of a mild solution for the fractional delay control system. Firstly, we will study the control problem. Then, we will deal with the topological structure of the solution set consisting of the compactness and Rσ property. We will derive a mild solution to the above delay control problem by using the Laplace transform method.
Fixed Point for Almost Contractions in v-Generalized b-Metric Spaces
Authors: Kadyan Anshuka , Rathee Savita , Kumar Anil , Rani Asha , Tas Kenan
Published: January 2023

We present a significant example to show that the class of v-generalized b-metric spaces properly contains the class of v-generalized metric spaces as well as b-metric spaces. This is accomplished because the example provided by Došenović et al. (2020) is insufficient to expose the generality of v-generalized b-metric spaces over the existing related spaces. Therefore, we establish fixed point theorems by defining generalized almost contractions of rational type and Reich type in v-generalized b-metric spaces. Moreover, we compare the proven results with the already existing fixed point theorems in this space by presenting suitable examples. As a consequence of these fixed point theorems, we further develop some common fixed-point results that ensure the existence and uniqueness of coincidence points and common fixed points for a pair of self maps. Finally, we use the outcome to check that the given Fredholm integral equation has a solution and that it is also unique.
The Haystack Telescope as an Astronomical Instrument
Authors: Kauffmann Jens , Rajagopalan Ganesh , Akiyama Kazunori , Fish Vincent , Lonsdale Colin , Matthews Lynn D. , Pillai Thushara G.S.
Published: January 2023

The Haystack Telescope is an antenna with a diameter of 37 m and an elevation-dependent surface accuracy of ≤100μm that is capable of millimeter-wave observations. The radome-enclosed instrument serves as a radar sensor for space situational awareness, with about one-third of the time available for research by MIT Haystack Observatory. Ongoing testing with the K-band (18–26 GHz) and W-band receivers (currently 85–93 GHz) is preparing the inclusion of the telescope into the Event Horizon Telescope (EHT) array and the use as a single-dish research telescope. Given its geographic location, the addition of the Haystack Telescope to current and future versions of the EHT array would substantially improve the image quality.
Early Educational Interventions to Prevent Gender-Based Violence: A Systematic Review
Authors: Villardón-Gallego Lourdes , García-Cid Alba , Estévez Ana , García-Carrión Rocío
Published: January 2023

Background: Gender-based violence is a worldwide public health problem that is increasingly occurring at younger ages. This investigation aims to analyze effective interventions to prevent and to face gender-based violence beginning in early childhood in order to ensure quality education for all children through violence-free schools. Methods: This research has conducted a systematic review of interventions that have demonstrated a positive impact on the prevention and reduction of gender-based violence from early ages up to 12 years, inclusive, in schools. An extensive search in scientific databases (WoS, SCOPUS, ERIC, PsycINFO) was conducted from 2007 to 2022. Results: Thirteen articles were selected and analyzed in-depth to identify the success factors of these interventions, which (a) are integrated into the school curriculum; (b) promote active participation of students and community; (c) are based on scientific evidence; and (d) make relevant adaptations to a specific group and context. Conclusions: The programs analyzed have had a positive impact on raising awareness of gender violence, overcoming stereotypes, improving relationships in the classroom and reducing violent behavior, as well as empowering the most vulnerable people.
Usefulness of YouTube in Sharing Information about New Gene Therapy for Spinal Muscular Atrophy: A Content Analysis
Authors: Kim Kyeong Yeol , Jang Chan Woong , Chung Seok Young , Kim Myungsang , Cho Sung-Rae , Cho Han Eol
Published: January 2023

This study aimed to objectively assess YouTube videos’ quality, reliability, and information delivery capability regarding novel spinal muscular atrophy treatments. Using the keywords “nusinersen”, “spinraza”, “ridisplam”, “evrysdi”, “onasemnogene abeparvovec”, and “zolgensma”, we were able to retrieve and screen 360 videos before settling on a final sample of 99 on 25 September 2022. Then, two independent raters used the mDISCERN and GQS instruments to evaluate the videos’ reliability and quality and the Information Delivery Capability (IDC) score to assess the videos’ accuracy and patient-friendliness. The quality, reliability, and information delivery capability of the videos about the new treatment for SMA were quite heterogeneous, with an average mDISCERN, GQS, and IDC score of 3.172 ± 0.899, 2.980 ± 1.025, and 4.141 ± 1.747, respectively. In-depth analysis showed that healthcare expert videos that explained contents while showing infographic supplements had good quality, reliability, and information delivery capability. As YouTube is already a dominant media platform, the public may obtain new information about novel therapeutics for SMA through YouTube. It is necessary to consider how SMA patients and caregivers can choose trusted sources with reliable information on YouTube, and our results can provide clues. Additionally, experts should strive to provide more accurate, reliable, and patient-oriented videos.
Reflectance Confocal Microscopy in Monitoring Atopic Dermatitis Treated with Topical Calcineurin Inhibitors
Authors: Bratu Dalia , Boda Daniel , Caruntu Constantin
Published: January 2023

Atopic dermatitis is a chronic inflammatory skin disease associated with multiple allergies in the atopic march. It has a complex pathogenesis, related to genetic, immune, and environmental factors. Its incidence and prevalence are increasing in the last decades, especially in developed countries. It affects the quality of life due to the recurrent lesions and the associated pruritus. Thus, it is very important to use non-invasive techniques to manage and follow-up the patients with such a heterogenous disease that can have a high impact on some of them. The reflectance confocal microscope is a modern device for in vivo visualization of the epidermis and the upper dermis which could replace in some cases the cutaneous biopsy. We report a case of a patient with atopic dermatitis investigated with the confocal reflectance microscope at the beginning of the topical treatment with calcineurin inhibitors and three weeks after, with favorable evolution. Reflectance confocal microscopy allows the assessment of the dynamic changes in the skin during treatment. Moreover, it can be useful for highlighting discrete changes even in the subclinical stages of the inflammatory process. Future developments, which will lead to the definition and validation of reflectance confocal microscopy criteria for the diagnosis and staging of atopic dermatitis, could help to improve the treatment and prevention strategies of the disease.
Exploration of COVID-19 Pandemic Prevention Behaviors among Healthcare Workers
Authors: Huang Hui-Ting , Tsai Chung-Hung , Wang Chia-Fen , Chien Tzu-Chao , Chang Shu-Hao
Published: January 2023

Since the outbreak of COVID-19, the pandemic has become an important topic of global public health. To reduce the rapid spread of the pandemic, compliance with preventive behaviors has become one of the important guidelines from the World Health Organization (WHO). Healthcare workers stand on the frontline for pandemic prevention, and preventive behaviors are essential measures to protect their health and safety. The purpose of this study was to propose an integrative model that explained and predicted COVID-19 preventive behaviors among healthcare workers. The study integrated workplace safety climate and the health belief model (HBM) to verify the impact of workplace safety climate and health belief factors on the safety attitude, safety compliance, and safety satisfaction of healthcare workers performing COVID-19 pandemic prevention behaviors. A cross-sectional study was conducted from March to August 2021 with a self-administered online questionnaire. The sample of the study was drawn from healthcare workers of a famous medical institution in Taipei City as research subjects. After collecting 273 valid questionnaires and verifying them through the analysis of structural equation modeling (SEM), the findings revealed that workplace safety climate had an impact on health belief factors, and then health belief factors had impacts on safety attitudes. In addition, safety attitude affected safety compliance, while safety compliance further affected safety satisfaction. The study showed that workplace safety climate can strengthen healthcare workers’ health beliefs and further affect their safety attitudes, safety compliance, and safety satisfaction. The study attempted to propose a model of healthcare workers’ pandemic prevention behaviors as a reference for medical facility administrators in real practice.
Classification of B-Cell Lymphomas and Immunodeficiency-Related Lymphoproliferations: What’s New?
Authors: Chadburn Amy , Gloghini Annunziata , Carbone Antonino
Published: January 2023

New insights from genomic studies have had an impact on the definition and the diagnosis of several lymphoid tumors including follicular B-cell lymphomas, aggressive diffuse large B-cell lymphomas, and lymphoproliferations associated with acquired and posttransplant immunodeficiencies. Follicular lymphoma (FL) includes tumors whose behavior varies widely from indolent/early lesions to aggressive/transformed lymphomas. Although some large B-cell lymphomas can be subclassified as specific entities, the majority lack the characteristics necessary for subclassification and, thus, are termed diffuse large B-cell lymphoma, NOS. There have been, however, some changes in the classification of specific subtypes of large B-cell lymphoma as well as the addition of new entities, a few of which are highlighted in this article. The immunodeficiency-related lymphoproliferative disorders are currently divided into four major categories based on the clinical setting in which they arose: primary immune deficiency, post-transplant, HIV infection, and iatrogenic immunosuppression. In the two upcoming classifications systems for hematolymphoid neoplasms, International Consensus Classification (ICC) and WHO-HAEM-5, there is a divergence in the approach to categorize these lesions. Furthermore, whereas the WHO-HAEM-5 confirms the ability to classify a spectrum of EBV+ lesions as EBV+ DLBCL, NOS, the ICC has separated out lesions that are composed of a heterogenous cellular infiltrate into a new separate category, “EBV-positive polymorphic B cell lymphoproliferative disorder, NOS”. Both WHO-HAEM-5 and ICC recognize a number of KSHV/HHV8-associated lymphoid lesions and acknowledge that there is significant overlap among the different lesions. In the future, translation of these innovations in general practice requires further validation.
In-Orchard Sizing of Mango Fruit: 2. Forward Estimation of Size at Harvest
Authors: Amaral Marcelo H. , Walsh Kerry B.
Published: January 2023

Forecast of tree fruit yield requires prediction of harvest time fruit size as well as fruit number. Mango (Mangifera indica L.) fruit mass can be estimated from correlation to measurements of fruit length (L), width (W) and thickness (T). On-tree measurements of individually tagged fruit were undertaken using callipers at weekly intervals until the fruit were past commercial maturity, as judged using growing degree days (GDD), for mango cultivars ‘Honey Gold’, ‘Calypso’ and ‘Keitt’ at four locations in Australia and Brazil during the 2020/21 and 21/22 production seasons. Across all cultivars, the linear correlation of fruit mass to LWT was characterized by a R2 of 0.99, RMSE of 29.9 g and slope of 0.5472 g/cm3, while the linear correlation of fruit mass to L((W+T)2)2, mimicking what can be measured by machine vision of fruit on tree, was characterized by a R2 of 0.97, RMSE of 25.0 g and slope of 0.5439 g/cm3. A procedure was established for the prediction of fruit size at harvest based on measurements made five and four or four and three weeks prior to harvest (approx. 514 and 422 GDD, before harvest, respectively). Linear regression models on weekly increase in fruit mass estimated from lineal measurements were characterized by an R2 > 0.88 for all populations, with an average slope (rate of increase) of 19.6 ± 7.1 g/week, depending on cultivar, season and site. The mean absolute percentage error for predicted mass compared to harvested fruit weight for estimates based on measurements of the earlier and later intervals was 16.3 ± 1.3% and 4.5 ± 2.4%, respectively. Measurement at the later interval allowed better accuracy on prediction of fruit tray size distribution. A recommendation was made for forecast of fruit mass at harvest based on in-field measurements at approximately 400 to 450 GDD units before harvest GDD and one week later.
Spatial–Temporal Data Imputation Model of Traffic Passenger Flow Based on Grid Division
Authors: Cai Li , Sha Cong , He Jing , Yao Shaowen
Published: January 2023

Traffic flows (e.g., the traffic of vehicles, passengers, and bikes) aim to reveal traffic flow phenomena generated by traffic participants in traffic activities. Various studies of traffic flows rely heavily on high-quality traffic data. The taxi GPS trajectory data are location data that include latitude, longitude, and time. These data are critical for traffic flow analysis, planning, infrastructure layout, and recommendations for urban residents. A city map can be divided into multiple grids according to the latitude and longitude coordinates, and traffic passenger flows data derived from taxi trajectory data can be extracted. However, random missing data occur due to weather and equipment failure. Therefore, the effective imputation of missing traffic flow data is a hot topic. This study proposes the spatio-temporal generative adversarial imputation net (ST-GAIN) model to solve the traffic passenger flows imputation. An adversarial game with multiple generators and one discriminator is established. The generator observes some components of the time-domain and regional traffic data vector extracted from the grid. It effectively imputes the missing values of the spatio-temporal traffic passenger flow data. The experimental data are accurate Kunming taxi trajectory data, and experimental results show that the proposed method outperforms five baseline methods regarding the imputation accuracy. It is significant and suggests the possibility of effectively applying the model to predict the passenger flows in some areas where traffic data cannot be collected for some reason or traffic data are randomly missing.
Morphology of Polymer Brushes in the Presence of Attractive Nanoparticles: Effects of Temperature
Authors: Eskandari Nasrabad Afshin , Laghaei Rozita , Coalson Rob D.
Published: January 2023

We study the role of temperature on the structure of pure polymer brushes and their mixture with attractive nanoparticles in flat and cylindrical geometries. It has previously been established that the addition of such nanoparticles causes the polymer brush to collapse and the intensity of the collapse depends on the attraction strength, the nanoparticle diameter, and the grafting density. In this work, we carry out molecular dynamics simulation under good solvent conditions to show how the collapse transition is affected by the temperature, for both plane grafted and inside-cylinder grafted brushes. We first examine the pure brush morphology and verify that the brush height is insensitive to temperature changes in both planar and cylindrical geometries, as expected for a polymer brush in a good solvent. On the other hand, for both system geometries, the brush structure in the presence of attractive nanoparticles is quite responsive to temperature changes. Generally speaking, for a given nanoparticle concentration, increasing the temperature causes the brush height to increase. A brush which contracts when nanoparticles are added eventually swells beyond its pure brush height as the system temperature is increased. The combination of two easily controlled external parameters, namely, concentration of nanoparticles in solution and temperature, allows for sensitive and reversible adjustment of the polymer brush height, a feature which could be exploited in designing smart polymer devices.
Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Authors: Duta-Cornescu Georgiana , Constantin Nicoleta , Pojoga Daniela-Maria , Nicuta Daniela , Simon-Gruita Alexandra
Published: January 2023

Cell and tissue plant cultures are used either to save vulnerable species from extinction or to multiply valuable genotypes, or both, and are widely applied for economically important plant species. For medicinal plants, the use of in vitro technologies for the production of secondary metabolites and pathogen-free plants has been greatly developed. Two opposite aspects characterize the in vitro micropropagation of medicinal plants: maintaining genetic fidelity for the perpetuation and preservation of elites, and the identification and exploitation of somaclonal variations associated with new, useful traits. A balance between what is advantageous and what is undesirable is necessary, and this implies the identification of somaclonal variability at all levels, from the phenotypic to molecular ones. This review addresses the somaclonal variation arising from the in vitro multiplication of medicinal plants from three perspectives: cytogenetics, genetics, and epigenetics. The possible causes of the appearance of somaclones, the methods for their identification, and the extent to which they are desirable are presented comparatively for different plant species with therapeutic properties. The emphasis is on the subtle changes at the genetic and epigenetic level, as it results from the application of methods based on DNA markers.
Polyphenol-Rich Beverage Consumption Affecting Parameters of the Lipid Metabolism in Healthy Subjects
Authors: Rahn Celina , Bakuradze Tamara , Stegmüller Simone , Galan Jens , Niesen Sonja , Winterhalter Peter , Richling Elke
Published: January 2023

Polyphenols are a diverse and widely distributed class of secondary metabolites, which possess numerous beneficial properties including a modulation of glucose and lipid metabolism. This placebo-controlled human intervention study was performed to explore effects of polyphenol-rich beverage (PRB) uptake on lipid metabolism, as well as DNA integrity. In this case, 36 healthy men were randomly divided to consume either 750 mL of a PRB (containing 51% chokeberry, cranberry, and pomegranate) or a placebo drink daily for eight weeks. Only PRB consumption was found to decrease fat and protein intakes significantly compared to the preceding one-week washout period. During the intervention with PRB an increased fat-free mass was shown after four weeks, whereas a significant elevation in body weight and leptin was observed in placebo group. Blood lipids were not significantly altered after PRB consumption, while triglyceride levels increased after placebo drink intake. In platelets, a significant inhibition of phosphodiesterase (PDE) activity was observed, more pronounced in test group. Consuming the PRB decreased total DNA strand breaks in whole blood as well as H2O2-induced breaks in isolated lymphocytes. Overall, our study suggested beneficial effects on lipid metabolism by reduced energy intake, modulation of biomarkers such as PDE activity and improved DNA integrity associated with PRB consumption.
The Double-Edged Sword of Oleuropein in Ovarian Cancer Cells: From Antioxidant Functions to Cytotoxic Effects
Authors: Scicchitano Stefania , Vecchio Eleonora , Battaglia Anna Martina , Oliverio Manuela , Nardi Monica , Procopio Antonio , Costanzo Francesco , Biamonte Flavia , Faniello Maria Concetta
Published: January 2023

Oleuropein plays a key role as a pro-oxidant as well as an antioxidant in cancer. In this study, the activity of oleuropein, in an in vitro model of ovarian (OCCs) and breast cancer cells (BCCs) was investigated. Cell viability and cell death were analyzed. Oxidative stress was measured by CM-H2DCFDA flow cytometry assay. Mitochondrial dysfunction was evaluated based on mitochondrial reactive oxygen species (ROS) and GPX4 protein levels. Further, the effects on iron metabolism were analyzed by measuring the intracellular labile iron pool (LIP). We confirmed that high doses of oleuropein show anti-proliferative and pro-apoptotic activity on HEY and MCF-7 cells. Moreover, our results indicate that low doses of oleuropein impair cell viability without affecting the mortality of cells, and also decrease the LIP and ROS levels, keeping them unchanged in MCF-7 cells. For the first time, our data show that low doses of oleuropein reduce erastin-mediated cell death. Interestingly, oleuropein decreases the levels of intracellular ROS and LIP in OCCs treated with erastin. Noteworthily, we observed an increased amount of ROS scavenging enzyme GPX4 together with a consistent reduction in mitochondrial ROS, confirming a reduction in oxidative stress in this model.