In vitro study on the effects of exogenic fibrolytic enzymes produced from &lt;i&gt;Trichoderma longibrachiatum&lt;/i&gt; on ruminal degradation of olive mill waste
Authors: Abid Khalil , Jabri Jihene , Yaich Hela , Malek Atef , Rekhis Jamel , Kamoun Mohamed 
Published: February 2022

Abstract. Olive mill waste is low-quality feed and rarely used in ruminant nutrition because of its high lignocellulose content, the existence of anti-nutritional factors such as total polyphenol and condensed tannin, and low protein contents. This in vitro research was conducted to valorize this waste (crude olive cake, extracted olive cake, and olive leaves) using an exogenous fibrolytic enzyme produced from Trichoderma longibrachiatum in ruminal nutrition. The enzymatic activity of this additive was 1161 units of endoglucanase per millilitre, 113 units of exoglucanase per millilitre, and 2267 units of xylanases per millilitre. This treatment was applied by spraying substrates with four doses: 0 (control), 1 (low), 2 (medium), and 4µL g−1 µL g−1 (high) of dry matter olive mill waste in an air-conditioned room at 26 ∘C for 12 h before in vitro incubation. For the crude olive cake, this additive at high doses increased degradation of 14 % of cellulose and 8 % of hemicellulose compared with the control at 12 h before the in vitro incubation. Consequently, it increased dry matter solubility and reduced sugars at this period compared to the control. Upon ruminal incubation, the high dose of exogenous fibrolytic enzyme increased the gas production from the immediately soluble fraction and insoluble fraction, the rate of gas production for the insoluble fraction, the dry matter degradability by 26 %, the organic matter degradability by 24 %, the metabolizable energy value by 28 %, and the microbial crude protein production by 24 % compared with the control. For olive leaves, an exogenous fibrolytic enzyme at medium dosage can also hydrolyse the hemicellulose compound, release fewer sugars, and increase dry matter solubility compared with the control at 12 h before the in vitro incubation. Upon in vitro incubation, the medium dose increased the gas production from immediately soluble and insoluble fractions, the rate of gas production for the insoluble fraction, the dry matter degradability by 13 %, the organic matter degradability by 11 %, the metabolizable energy value by 12 %, and the microbial crude protein production by 12 % compared with the control. However, the highest dose altered the gas production from insoluble fractions and decreased microbial crude protein production by 6 % compared with the control. Under the same conditions, an exogenous fibrolytic enzyme applied to extracted olive cake did not produce any effect in the chemical composition and nutritional value. These results showed clearly that effectiveness of exogenous fibrolytic enzyme varied with incubated waste. Increasing the nutritional value of crude olive cake and olive leaves using an exogenous fibrolytic enzyme can encourage breeders to use this waste as feed at a low cost in animal nutrition. This valorization of waste is a good solution to reduce pollution of soils and groundwater caused by throwing out this polluted waste into the environment.