bluuebunny/crossref_metadata_2025 huggingface dataset:
A Retrospective Survey of the Abortion Outbreak Event Caused by Brucellosis at a Blue Fox Breeding Farm in Heilongjiang Province, China
Authors: Zhou Yulong , Meng Ye , Ren Yachao , Liu Zhiguo , Li Zhenjun
Published: June 2021

Brucellosis is a common zoonosis in China, resulting in abortion in animals. Outbreaks of abortion in blue foxes caused by Brucella infection have rarely been reported. In the present study, 3–5 mL blood samples collected from the femoral veins of 10 abortuses of blue foxes were assessed by RBPT (Rose Bengal plate test) and SAT (serum tube agglutination test) to preliminarily investigate the source of infection for the clustering of abortion events at a blue fox farm in Heilongjiang Province. Screening experiments showed that all 10 blood samples were positive in the RBPT, while only eight blood samples out of the 10 were positive in the SAT. Subsequently, 10 tissue samples (spleen, lungs, stomach contents, and afterbirth) from the same 10 foxes were assessed using AMOS (acronym for B. abortus, melitensis, ovis, and suis)-PCR (polymerase chain reaction), and sequencing analysis was performed on amplification products to verify the results of the serology survey. Results showed a spectral band of ~731 bp in these samples. BLAST showed sequences of AMOS-PCR products in this study to be 100% similar (E = 0.0) to sequences in B. melitensis strain from GenBank. These data preliminarily indicated that the blue fox's outbreak of abortion events was caused by brucellosis via the B. melitensis strain. Then 726 serum samples were tested by RBPT and SAT to determine the prevalence of brucellosis on the farm. A comprehensive epidemiological and reproductive status survey of the infected blue fox population was performed. The seropositive rate was found to be 67.90% (493/726) by RBPT and 41.32% (300/726) by SAT. The technicians had stopped feeding the foxes with chicken carcasses and instead fed them raw ground sheep organs (lungs, tracheae, placentae, and dead sheep fetuses) infected by B. meliteneis strains, and that this change in diet caused the outbreak of abortion events. The high abortion rate (55%) and low cub survival rate (65%) were the most distinctive features of the outbreak; these factors led to severe economic losses. Feeding cooked sheep/goat offal and strict breeding management is necessary for disease prevention.
Establishment of a Multiplex RT-PCR Method for the Detection of Five Known Genotypes of Porcine Astroviruses
Authors: Liu Xin , Zhang Wenchao , Wang Dongjing , Zhu Xinyue , Chen Ying , Ouyang Kang , Wei Zuzhang , Liu Huan , Huang Weijian
Published: June 2021

Porcine astroviruses (PAstVs) are prevalent in pigs worldwide, and five genotypes have been reported to circulate in China. However, little is known about the coinfection status of PAstVs. For differential and simultaneous diagnoses of these five genotypes of PAstVs, a multiplex RT-PCR method was established on the basis of the ORF2 gene of type 1 PAstV, and the ORF1ab genes of type two to five PAstVs. This quintuple PCR system was developed through optimization of multiplex PCR and detection sensitivity and specificity. The results showed that this multiplex RT-PCR method could specifically detect all the five PAstV genotypes without cross-reaction to any other major viruses circulating in Chinese pig farms. The detection limit of this method was as low as 10 pg of standard plasmids of each PAstV genotype. In addition, a total of 275 fecal samples collected from different districts of Guangxi, China, between April 2019 and November 2020, were tested by this newly established multiplex RT-PCR. Moreover, the sensitivity and specificity of monoplex and multiplex RT-PCR methods were compared by detecting the same set of clinical positive samples. The results revealed that PAstV1 (31/275), PAstV2 (49/275), PAstV3 (36/275), PAstV4 (41/275), and PAstV5 (22/275) were all detected, and dual (PAstV1+PAstV2, PAstV1+PAstV3, PAstV2+PAstV3, PAstV2+PAstV4, PAstV3+PAstV4, and PAstV4+PAstV5) or triple genotypes (PAstV1+PAstV2+PAstV3 and PAstV2+PAstV3+PAstV4) of coinfections were also unveiled in this study. The detection result of multiplex PCR was consistent with that of monoplex PCR. Compared with monoplex PCR, this multiplex PCR method showed obvious advantages such as time and cost efficiency and high sensitivity and specificity. This multiplex RT-PCR method offered a valuable tool for the rapid and accurate detection of PAstV genotypes circulating in pig herds and will facilitate the surveillance of PAstV coinfection status.
Citizen Coproduction and Social Media Communication: Delivering a Municipal Government’s Urban Services through Digital Participation
Authors: Cho Wonhyuk , Melisa Winda Dwi
Published: June 2021

This study investigated how social media is used by a municipal government agency for communication of citizen coproduction initiatives, through social media content analysis of the government’s official Twitter account. This article identified that the dominant form of social media coproduction in the Bandung municipal government in Indonesia is government-to-citizen (G2C) interaction, focused primarily on informing and nudging (86.62%) citizens, as well as some limited elements of citizen-to-government (C2G) communication, such as citizen sourcing and citizen reporting (8.96%). The municipal government uses various visual tools on Twitter to disseminate G2C information and convey its messages. Regarding the phase of the service cycle, this study found that the majority of social media communications are related to co-assessment (52.26%) and co-designing (42.24%), with a limited number of tweets about co-delivery (3.25%). Based on these findings, this article discusses the shifting relationship between government and citizens brought on by the adoption of this social media platform in its service delivery arrangement.
Dose–Response Effects of 3-Nitrooxypropanol Combined with Low- and High-Concentrate Feed Proportions in the Dairy Cow Ration on Fermentation Parameters in a Rumen Simulation Technique
Authors: Schilde Matthias , von Soosten Dirk , Hüther Liane , Kersten Susanne , Meyer Ulrich , Zeyner Annette , Dänicke Sven
Published: June 2021

Methane (CH4) from ruminal feed degradation is a major pollutant from ruminant livestock, which calls for mitigation strategies. The purpose of the present 4 × 2 factorial arrangement was to investigate the dose–response relationships between four doses of the CH4 inhibitor 3-nitrooxypropanol (3-NOP) and potential synergistic effects with low (LC) or high (HC) concentrate feed proportions (CFP) on CH4 reduction as both mitigation approaches differ in their mode of action (direct 3-NOP vs. indirect CFP effects). Diet substrates and 3-NOP were incubated in a rumen simulation technique to measure the concentration and production of volatile fatty acids (VFA), fermentation gases as well as substrate disappearance. Negative side effects on fermentation regarding total VFA and gas production as well as nutrient degradability were observed for neither CFP nor 3-NOP. CH4 production decreased from 10% up to 97% in a dose-dependent manner with increasing 3-NOP inclusion rate (dose: p < 0.001) but irrespective of CFP (CFP × dose: p = 0.094). Hydrogen gas accumulated correspondingly with increased 3-NOP dose (dose: p < 0.001). In vitro pH (p = 0.019) and redox potential (p = 0.066) varied by CFP, whereas the latter fluctuated with 3-NOP dose (p = 0.01). Acetate and iso-butyrate (mol %) decreased with 3-NOP dose, whereas iso-valerate increased (dose: p < 0.001). Propionate and valerate varied inconsistently due to 3-NOP supplementation. The feed additive 3-NOP was proven to be a dose-dependent yet effective CH4 inhibitor under conditions in vitro. The observed lack of additivity of increased CFP on the CH4 inhibition potential of 3-NOP needs to be verified in future research testing further diet types both in vitro and in vivo.
Cryptosporidium spp. Infections in Combination with Other Enteric Pathogens in the Global Calf Population
Authors: Conrady Beate , Brunauer Michael , Roch Franz-Ferdinand
Published: June 2021

The most common worldwide diarrhoea-causing agents in neonatal calves are Cryptosporidium spp. (Crypto), bovine rotavirus (BRV), bovine coronavirus (BCoV), and enterotoxigenic Escherichia coli F5 (K99) (ETEC). Crypto is a zoonotic pathogen of diarrhoea in humans, particularly for children and immunocompromised adults. Four weighted-stratified random-effect meta-analyses including meta-regression analyses were performed to calculate the worldwide mean prevalence of Crypto and associated concurrent infections with BRV, BCoV and ETEC, as well as their potential influencing factors. The meta-analysis incorporated 28 studies (56 substudies) in 17 countries that determined the presence or absence of concurrent infections with Crypto in the global calf population. Approximately half of all considered studies presented here were conducted in Europe independently of the type of infections with Crypto. Within Europe, the highest estimated mean Crypto-BRV prevalence was identified in Ireland (16.7%), the highest estimated mean Crypto-BCoV prevalence was detected in the United Kingdom (4.3%), and the highest estimated mean Crypto-ETEC prevalence across the literature was determined in Turkey (4.7%). The chance of detecting BRV, BCoV, and ETEC in calves with diarrhoea was 0.8 (confidence interval (CI): 0.6–1.0), 0.7 (CI: 0.5–1.0) and 0.6 (CI: 0.4–0.9) lower in the presence of Crypto compared to calves without Crypto. This may indicate an inhibitory effect between BRV, BCoV, ETEC, and Crypto in calves. The variance in the published prevalence across the literature can mainly be explained by the “diagnostic” factor (R2 min–max: 0.0–40.3%), followed by the “health status of the sampled animals” (R2 min–max: 1.4–27.3%) and “geographical region” (R2 min–max: 5.9–23.6%).
Unmitigated Surgical Castration in Calves of Different Ages: Electroencephalographic and Neurohormonal Findings
Authors: Bergamasco Luciana , Edwards-Callaway Lily N. , Bello Nora M. , Mijares Sage , Cull Charley A. , Mosher Ruby A. , Coetzee Johann F.
Published: June 2021

Castration is a common management procedure employed in North American cattle production and is known to cause a pain response. The present study was designed to investigate the effect of unmitigated surgical castration on the electroencephalography (EEG) responses and plasma substance P (SP) concentrations in calves of different ages under the same experimental conditions. Thirty male Holstein calves in three age categories [<6 weeks (6W); 3 months (3M); 6 months (6M); 10 calves per age group] were used in the study. Calves were subjected to a simulated castration session (SHAM) followed 24 h later by surgical castration (CAST) without analgesia. An EEG analysis was performed before the procedure (i.e., baseline), at treatment, and 0–5, 5–10, and 10–20 min post-treatment for both SHAM and CAST, respectively. Blood samples were collected immediately prior to both treatments (time 0) and again at 1, 2, 4, 8, and 12 h after both treatments. The EEG results showed a three-way interaction between treatment, age, and time for delta and beta absolute power, beta relative power, total power, and median frequency (p = 0.004, p = 0.04, p = 0.04, p = 0.03, and p = 0.008, respectively). Following CAST, EEG total power decreased, and median frequency increased relative to SHAM in 6W and 3M calves only following treatment. For 6W and 3M calves, delta and beta absolute power increased at CAST and at later time points relative to SHAM. Marginal evidence for two-way interactions was noted between time and treatment and between age and treatment on the concentration of SP (p = 0.068 and p = 0.066, respectively). Substance P concentrations decreased in CAST treatment compared to SHAM at the later times (8 h: p = 0.007; 12 h: p = 0.048); 6W calves showed lower SP concentration at CAST relative to SHAM (p = 0.017). These findings indicate variation in EEG responses and in SP concentrations following unmitigated surgical castration in calves and that these responses may be age specific. These EEG findings have implications for supporting the perception of the pain associated with surgical castration in young calves and emphasize the urgency of pain mitigation strategies during routine husbandry practices such as castration, as typically implemented in North American cattle management.
Whole-Genome Sequencing Investigation of a Large Nosocomial Outbreak Caused by ST131 H30Rx KPC-Producing Escherichia coli in Italy
Authors: Piazza Aurora , Principe Luigi , Comandatore Francesco , Perini Matteo , Meroni Elisa , Mattioni Marchetti Vittoria , Migliavacca Roberta , Luzzaro Francesco
Published: June 2021

KPC-producing Escherichia coli (KPC-Ec) remains uncommon, being mainly reported as the cause of sporadic episodes of infection rather than outbreak events. Here we retrospectively describe the dynamics of a large hospital outbreak sustained by KPC-Ec, involving 106 patients and 25 hospital wards, during a six-month period. Twenty-nine representative KPC-Ec isolates (8/29 from rectal swabs; 21/29 from other clinical specimens) have been investigated by Whole-Genome Sequencing (WGS). Outbreak isolates showed a multidrug-resistant profile and harbored several resistance determinants, including blaCTX-M-27, aadA5, dfrA17, sulI, gyrA1AB and parC1aAB. Phylogenomic analysis identified the ST131 cluster 1 (23/29 isolates), H30Rx clade C, as responsible for the epidemic event. A further two KPC-Ec ST131 clusters were identified: cluster 2 (n = 2/29) and cluster 3 (n = 1/29). The remaining KPC-Ec resulted in ST978 (n = 2/29) and ST1193 (n = 1/29), and were blaKPC-3 associated. The KPC-Ec ST131 cluster 1, originated in a previous KPC-Kp endemic context probably by plasmid transfer, and showed a clonal dissemination strategy. Transmission of the blaKPC gene to the globally disseminated high-risk ST131 clone represents a serious cause of concern. Application of WGS in outbreak investigations could be useful to better understand the evolution of epidemic events in order to address infection control and contrast interventions, especially when high-risk epidemic clones are involved.
Effects of Vitamin B12 Deficiency on Amyloid-β Toxicity in Caenorhabditis elegans
Authors: Andra Arif , Tanigawa Shoko , Bito Tomohiro , Ishihara Atsushi , Watanabe Fumio , Yabuta Yukinori
Published: June 2021

High homocysteine (Hcy) levels, mainly caused by vitamin B12 deficiency, have been reported to induce amyloid-β (Aβ) formation and tau hyperphosphorylation in mouse models of Alzheimer’s disease. However, the relationship between B12 deficiency and Aβ aggregation is poorly understood, as is the associated mechanism. In the current study, we used the transgenic C. elegans strain GMC101, which expresses human Aβ1–42 peptides in muscle cells, to investigate the effects of B12 deficiency on Aβ aggregation–associated paralysis. C. elegans GMC101 was grown on nematode growth medium with or without B12 supplementation or with 2-O-α-D-glucopyranosyl-L-ascorbic acid (AsA-2G) supplementation. The worms were age-synchronized by hypochlorite bleaching and incubated at 20 °C. After the worms reached the young adult stage, the temperature was increased to 25 °C to induce Aβ production. Worms lacking B12 supplementation exhibited paralysis faster and more severely than those that received it. Furthermore, supplementing B12-deficient growth medium with AsA-2G rescued the paralysis phenotype. However, AsA-2G had no effect on the aggregation of Aβ peptides. Our results indicated that B12 supplementation lowered Hcy levels and alleviated Aβ toxicity, suggesting that oxidative stress caused by elevated Hcy levels is an important factor in Aβ toxicity.
Preharvest Treatment with Oxalic Acid Improves Postharvest Storage of Lemon Fruit by Stimulation of the Antioxidant System and Phenolic Content
Authors: Serna-Escolano Vicente , Giménez María José , Castillo Salvador , Valverde Juan Miguel , Martínez-Romero Domingo , Guillén Fabián , Serrano María , Valero Daniel , Zapata Pedro Javier
Published: June 2021

Lemon trees (Citrus limon (L.) Burm. F) were treated monthly with oxalic acid (OA) at 0.1, 0.5, and 1 mM from initial fruit growth on the tree until harvest in2019. The experiment was repeated in 2020, with the application of OA 1 mM (according to the best results of 2019). In both years, fruit from OA-treated trees and the controls were stored for 35 days at 10 °C. Results showed that all treatments reduced weight loss (WL) and maintained higher firmness, total soluble solids (TSS), and total acidity (TA) than in the controls. Meanwhile, colour (hue angle) did not show significant differences. The activity of antioxidant enzymes, catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD) in the flavedo of the fruit from the OA-treated trees was higher than in the controls at harvest and after 35 days of storage. Similarly, the total phenolic content (TPC) in the flavedo and juice of the fruit from the OA-treated trees were higher than in the controls. The increase in the activity of the antioxidant enzymes and TPC started with the first preharvest OA treatment and were maintained during fruit development on the tree until harvest. Preharvest OA treatments enhanced the antioxidant system of the lemon fruits, reducing the postharvest incidence of decay. Thus, OA could be a useful tool to increase the quality and functional properties of lemon fruits.
Method of Selecting the Means of Transport of the Winning, Taking into Account Environmental Aspects
Authors: Kowalski Arkadiusz , Waszkowski Robert
Published: June 2021

The transport of the winning in deep mines, using the room and pillar mining system, is most often performed with bucket loaders and haul trucks. In the era of attempts to stop rapid climate change, it is crucial to choose the transport means for the winning both in terms of efficiency and cost-effectiveness and to consider its environmental aspect. Permissible levels of pollutant emissions in exhaust gases are defined for this type of means of transport by the EU Stage Standards. There is a discernible need to develop a multi-criteria method supporting the decision-making process, which should reward loaders and haul trucks that meet more stringent emission standards. The article proposes an innovative idea of taking environmental aspects into account when selecting loaders and haul trucks for excavated material transport tasks, so that the amount of pollutants emitted by them in exhaust gases, e.g., the sum of hydrocarbons and nitrogen oxides (HC+NOx), is also taken into consideration when assigning means of transport to particular tasks. Based on simulation studies for a specific case, it was found that a 20% reduction of HC+NOx emission is possible with only a 2% increase in the transport costs of the winning. For this purpose, an objective function was used formulated on the basis of two criteria: minimization of the transport cost of the winning and the level of pollutant emissions in the exhaust gases. Since dozens of mining machines are operated continuously in deep mines of non-ferrous metal ores, the application of the proposed method would significantly reduce the emission of pollutants in the used air coming out of ventilation shafts.
Editorial for the Special Issue “Extracellular Chaperones and Related miRNA as Diagnostic Tools of Chronic Diseases”
Authors: Marino Claudia , Gorska-Ponikowska Magdalena , Rappa Francesca , Cappello Francesco
Published: June 2021

Molecular chaperones are a family of proteins that are highly conserved during phylogenesis [...]
New Subclass Framework and Concrete Examples of Strongly Asymmetric Public Key Agreement
Authors: Iriyama Satoshi , Jimbo Koki , Regoli Massimo
Published: June 2021

Strongly asymmetric public key agreement (SAPKA) is a class of key exchange between Alice and Bob that was introduced in 2011. The greatest difference from the standard PKA algorithms is that Bob constructs multiple public keys and Alice uses one of these to calculate her public key and her secret shared key. Therefore, the number of public keys and calculation rules for each key differ for each user. Although algorithms with high security and computational efficiency exist in this class, the relation between the parameters of SAPKA and its security and computational efficiency has not yet been fully clarified. Therefore, our main objective in this study was to classify the SAPKA algorithms according to their properties. By attempting algorithm attacks, we found that certain parameters are more strongly related to the security. On this basis, we constructed concrete algorithms and a new subclass of SAPKA, in which the responsibility of maintaining security is significantly more associated with the secret parameters of Bob than those of Alice. Moreover, we demonstrate 1. insufficient but necessary conditions for this subclass, 2. inclusion relations between the subclasses of SAPKA, and 3. concrete examples of this sub-class with reports of implementational experiments.
From Classical Machine Learning to Deep Neural Networks: A Simplified Scientometric Review
Authors: Mukhamediev Ravil I. , Symagulov Adilkhan , Kuchin Yan , Yakunin Kirill , Yelis Marina
Published: June 2021

There are promising prospects on the way to widespread use of AI, as well as problems that need to be overcome to adapt AI&ML technologies in industries. The paper systematizes the AI sections and calculates the dynamics of changes in the number of scientific articles in machine learning sections according to Google Scholar. The method of data acquisition and calculation of dynamic indicators of changes in publication activity is described: growth rate (D1) and acceleration of growth (D2) of scientific publications. Analysis of publication activity, in particular, showed a high interest in modern transformer models, the development of datasets for some industries, and a sharp increase in interest in methods of explainable machine learning. Relatively small research domains are receiving increasing attention, as evidenced by the negative correlation between the number of articles and D1 and D2 scores. The results show that, despite the limitations of the method, it is possible to (1) identify fast-growing areas of research regardless of the number of articles, and (2) predict publication activity in the short term with satisfactory accuracy for practice (the average prediction error for the year ahead is 6%, with a standard deviation of 7%). This paper presents results for more than 400 search queries related to classified research areas and the application of machine learning models to industries. The proposed method evaluates the dynamics of growth and the decline of scientific domains associated with certain key terms. It does not require access to large bibliometric archives and allows to relatively quickly obtain quantitative estimates of dynamic indicators.
Designing Parallel Adaptive Laplacian Smoothing for Improving Tetrahedral Mesh Quality on the GPU
Authors: Xi Ning , Sun Yingjie , Xiao Lei , Mei Gang
Published: June 2021

Mesh quality is a critical issue in numerical computing because it directly impacts both computational efficiency and accuracy. Tetrahedral meshes are widely used in various engineering and science applications. However, in large-scale and complicated application scenarios, there are a large number of tetrahedrons, and in this case, the improvement of mesh quality is computationally expensive. Laplacian mesh smoothing is a simple mesh optimization method that improves mesh quality by changing the locations of nodes. In this paper, by exploiting the parallelism features of the modern graphics processing unit (GPU), we specifically designed a parallel adaptive Laplacian smoothing algorithm for improving the quality of large-scale tetrahedral meshes. In the proposed adaptive algorithm, we defined the aspect ratio as a metric to judge the mesh quality after each iteration to ensure that every smoothing improves the mesh quality. The adaptive algorithm avoids the shortcoming of the ordinary Laplacian algorithm to create potential invalid elements in the concave area. We conducted 5 groups of comparative experimental tests to evaluate the performance of the proposed parallel algorithm. The results demonstrated that the proposed adaptive algorithm is up to 23 times faster than the serial algorithms; and the accuracy of the tetrahedral mesh is satisfactorily improved after adaptive Laplacian mesh smoothing. Compared with the ordinary Laplacian algorithm, the proposed adaptive Laplacian algorithm is more applicable, and can effectively deal with those tetrahedrons with extremely poor quality. This indicates that the proposed parallel algorithm can be applied to improve the mesh quality in large-scale and complicated application scenarios.
Microbots Gene Delivery System Based on Bifidobacteria in a Tumor Model
Authors: Terrazas Armendáriz Luis D. , Luna Cruz Itza E. , Alvizo Báez Cynthia A. , Cavazos Jaramillo Azael A. , Rodríguez Padilla Cristina , Tamez-Guerra Reyes S. , Alcocer González Juan M.
Published: June 2021

In cancer, the use of microbots based on anaerobic bacteria as specific transporters targeting tumor tissues has been explored since most solid tumors exhibit hypoxic regions. The aim of this study was to develop and characterize magnetic microbots based on Bifidobacteria and iron oxide fluorescent magnetic nanoparticles complexed with chitosan and a hypoxia inducible plasmid. In addition, the efficiency of the microbots for gene delivery to solid tumors was evaluated in an in vivo model by florescence and luminescence. To elaborate microbots, iron oxide fluorescent magnetic nanoparticles complexed with chitosan and a hypoxia-inducible plasmid called nanocomplex (NCs) with a size of 302 nm and a ζ potential of +16 mV were obtained and loaded onto Bifidobacteria membranes. Microbots with a diameter between 1–2 µm were characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Microbots were injected intravenously through the tail vein to tumor-bearing mice, and then a magnet was placed to focus them to the tumor area. Forty-eight hours after injection, the biodistribution was determined by florescence and luminescence. The greatest luminescence and fluorescence emitted were found in tumor tissue compared with the normal organs. We created a vector that can be directed by a magnet and deliver genes whose expression is regulated by hypoxic microenvironment of tumor.
Physicochemical Changes Occurring during Long-Time Fermentation of the Indigenous Alcoholic Sorghum-Based Beverages Brewed in Northern Cameroon
Authors: Bayoï James Ronald , Etoa François-Xavier
Published: June 2021

In Cameroon, alcoholic beverages represent one of the main consumed drinks. In northern regions, indigenous sorghum beers are very popular and widely consumed in an actively fermenting state by people. In this study, some physicochemical parameters of alcoholic sorghum beverages and correlations between them were evaluated during fermentation for 10 days. The indigenous white and red beers were produced at the laboratory scale assisted by experimental producers and some parameters (pH, total acidity, alcohol, sugars, density, total solids, temperature, and conductivity) were measured on the wort and fermented beverages. The pH decreased from 3.2 to 2.4 and 3.11 to 2.41; total acidity increased from 1.07 to 5.1 g/L and 0.5 to 4.6 g/L; alcohol was enhanced from 0 to 9.5% and 0 to 6.8% (v/v); total solids dropped from 13.6 to 5°P and 12.2 to 3.3°P, respectively, in the white and red sorghum beers. The multivariate analysis showed a good correlation between consumption of sugar, the decrease in total solids and density with the decrease in pH. Additionally, it was shown that a perfect link exists between the production of alcohol and organic acids. The hierarchical analysis showed that indigenous red beer samples fermented for one and two days and those fermented for four to 10 days were related and could be separate in two distinct groups, whereas white turbid beer samples were separated in three different groups, those fermented for one to four days, five to six days, and seven to 10 days. The results obtained could serve as a guide to better understand the fermentation process of indigenous alcoholic sorghum-based beverages.
Walnut (J. regia) Agro-Residues as a Rich Source of Phenolic Compounds
Authors: Medic Aljaz , Jakopic Jerneja , Solar Anita , Hudina Metka , Veberic Robert
Published: June 2021

The present study was designed to identify and quantify the major phenolic compounds (phenolics) in the inner and outer husks, buds and bark of the Persian walnut, Juglans regia L. A comparison across six different cultivars grown in Slovenia was also carried out: ‘Fernor’, ‘Fernette’, ‘Franquette’, ‘Sava’, ‘Krka’ and ‘Rubina’. A total of 83 compounds were identified, which included 25 naphthoquinones, 15 hydroxycinnamic acids, 8 hydroxybenzoic acids, 13 flavanols, 2 flavones, 1 flavanone and 19 flavonols. For the first time, 38 phenolics in the husks, 57 phenolics in the buds and 29 phenolics in the bark were presented in J. regia within this study. Naphthoquinones were the major phenolics determined, approximately 75% of all analysed phenolics in the inner husk, 85% in the outer husk, 50% in buds and 80% in bark. The highest content of phenolics was found in the walnut buds, followed by the bark, the inner husk and the outer husk. On the basis of these high phenolic contents, walnut husks, buds and bark represented valuable by-products of the walnut tree. These data also show origin-related phenolic contents across the cultivars, and thus these phenolic profiles might serve to define the origins of different walnut cultivars.
Addressing Discrepancies between Experimental and Computational Procedures
Authors: Toma Milan , Guru Satvinder K. , Wu Wayne , Ali May , Ong Chi Wei
Published: June 2021

Imaging subject-specific heart valve, a crucial step to its design, has experimental variables that if unaccounted for, may lead to erroneous computational analysis and geometric errors of the resulting model. Preparation methods are developed to mitigate some sources of the geometric error. However, the resulting 3D geometry often does not retain the original dimensions before excision. Inverse fluid–structure interaction analysis is used to analyze the resulting geometry and to assess the valve’s closure. Based on the resulting closure, it is determined if the geometry used can yield realistic results. If full closure is not reached, the geometry is adjusted adequately until closure is observed.
Comment on Kobroob et al. Effectiveness of N-Acetylcysteine in the Treatment of Renal Deterioration Caused by Long-Term Exposure to Bisphenol A. Biomolecules 2021, 11, 655
Authors: Yan Liang-Jun
Published: June 2021

Bisphenol A (BPA: 2,2-bis-(4-hydroxyphenyl)-propane) is an industrial chemical that is widely used in the production of epoxy resins and polycarbonate for food containers and plastic bottles [...]
Comprehensive Evaluation of PAXgene Fixation on Oral Cancer Tissues Using Routine Histology, Immunohistochemistry, and FTIR Microspectroscopy
Authors: Lahiri Pooja , Mukherjee Suranjana , Ghosh Biswajoy , Das Debnath , Lahiri Basudev , Varshney Shailendra Kumar , Pal Mousumi , Paul Ranjan Rashmi , Chatterjee Jyotirmoy
Published: June 2021

The choice of tissue fixation is critical for preserving the morphology and biochemical information of tissues. Fragile oral tissues with lower tensile strength are challenging to process for histological applications as they are prone to processing damage, such as tissue tear, wrinkling, and tissue fall-off from slides. This leads to loss of morphological information and unnecessary delay in experimentation. In this study, we have characterized the new PAXgene tissue fixation system on oral buccal mucosal tissue of cancerous and normal pathology for routine histological and immunohistochemical applications. We aimed to minimize the processing damage of tissues and improve the quality of histological experiments. We also examined the preservation of biomolecules by PAXgene fixation using FTIR microspectroscopy. Our results demonstrate that the PAXgene-fixed tissues showed significantly less tissue fall-off from slides. Hematoxylin and Eosin staining showed comparable morphology between formalin-fixed and PAXgene-fixed tissues. Good quality and slightly superior immunostaining for cancer-associated proteins p53 and CK5/6 were observed in PAXgene-fixed tissues without antigen retrieval than formalin-fixed tissues. Further, FTIR measurements revealed superior preservation of glycogen, fatty acids, and amide III protein secondary structures in PAXgene-fixed tissues. Overall, we present the first comprehensive evaluation of the PAXgene tissue fixation system in oral tissues. This study concludes that the PAXgene tissue fixation system can be applied to oral tissues to perform diagnostic molecular pathology experiments without compromising the quality of the morphology or biochemistry of biomolecules.
Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis—The Importance of Biomarkers of the Inflammatory Reaction Involving Eosinophils
Authors: Sarbinowska Joanna , Wiatrak Benita , Waśko-Czopnik Dorota
Published: June 2021

Background: Invasive and costly endoscopic diagnosis is obligatory for the diagnosis and monitoring of eosinophilic esophagitis (EoE). This study aims to evaluate the usefulness of serum biomarkers involved in eosinophil-mediated inflammation in the management of EoE. Methods: A prospective cohort study was conducted in 58 patients with dysphagia. Each participant completed a health questionnaire, underwent esophagogastroduodenoscopy with esophageal biopsy for histopathological examination and assessment of total, inflammatory and fibrostenotic Eosinophilic Esophagitis Reference Score (EREFS). Serum levels of interleukin 5 (IL-5), interleukin 13 (IL-13), transforming growth factor β1 (TGF-β1), major basic protein (MBP), and eotaxin 3 were determined by enzyme immunoassays. Total of 16 patients meeting the histological criteria for EoE were treated with proton pump inhibitors for 8 weeks, and then the same diagnostics was performed again. Results: Statistically significantly higher concentrations of MBP and TGF-β1 were demonstrated in the group of patients with EoE, while MBP and eotaxin 3 correlated with the peak eosinophil count (PEC). Baseline MBP levels and eotaxin 3 after treatment significantly positively correlated with EREFS. There was a negative correlation between IL-13 and fibrostenotic EREFS. Additionally, after treatment, a negative correlation TGF-β1 was noted with the inflammatory EREFS and a positive correlation with the fibrostenotic EREFS. Conclusions: The potential role of MBP in predicting the diagnosis of EoE, eotaxin 3 in predicting the advancement and correlation of IL-13 and TGF-β1 in differentiating the inflammatory and fibrotic course of the disease may facilitate the management and individualization of EoE therapy.
Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research
Authors: Zachleder Vilém , Ivanov Ivan N. , Kselíková Veronika , Bialevich Vitali , Vítová Milada , Ota Shuhei , Takeshita Tsuyoshi , Kawano Shigeyuki , Bišová Kateřina
Published: June 2021

Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures of the chlorococcal green alga Parachlorella kessleri to study its growth and pattern of cell division under varying light intensities. The time courses of DNA replication, nuclear and cellular division, cell size, total RNA, protein content, dry matter and accumulation of starch were observed at incident light intensities of 110, 250 and 500 µmol photons m−2s−1. Furthermore, we studied the effect of deuterated water on Parachlorella kessleri growth and division, to mimic the effect of stress. We describe a novel multiple fission cell cycle pattern characterized by multiple rounds of DNA replication leading to cell polyploidization. Once completed, multiple nuclear divisions were performed with each of them, immediately followed by protoplast fission, terminated by the formation of daughter cells. The multiple fission cell cycle was represented by several consecutive doublings of growth parameters, each leading to the start of a reproductive sequence. The number of growth doublings increased with increasing light intensity and led to division into more daughter cells. This study establishes the baseline for cell cycle research at the molecular level as well as for potential biotechnological applications, particularly directed synthesis of (deuterated) starch and/or neutral lipids as carbon and energy reserves.
Self-Supporting Hydrogels Based on Fmoc-Derivatized Cationic Hexapeptides for Potential Biomedical Applications
Authors: Diaferia Carlo , Rosa Elisabetta , Gallo Enrico , Smaldone Giovanni , Stornaiuolo Mariano , Morelli Giancarlo , Accardo Antonella
Published: June 2021

Peptide-based hydrogels (PHGs) are biocompatible materials suitable for biological, biomedical, and biotechnological applications, such as drug delivery and diagnostic tools for imaging. Recently, a novel class of synthetic hydrogel-forming amphiphilic cationic peptides (referred to as series K), containing an aliphatic region and a Lys residue, was proposed as a scaffold for bioprinting applications. Here, we report the synthesis of six analogues of the series K, in which the acetyl group at the N-terminus is replaced by aromatic portions, such as the Fmoc protecting group or the Fmoc-FF hydrogelator. The tendency of all peptides to self-assemble and to gel in aqueous solution was investigated using a set of biophysical techniques. The structural characterization pointed out that only the Fmoc-derivatives of series K keep their capability to gel. Among them, Fmoc-K3 hydrogel, which is the more rigid one (G’ = 2526 Pa), acts as potential material for tissue engineering, fully supporting cell adhesion, survival, and duplication. These results describe a gelification process, allowed only by the correct balancing among aggregation forces within the peptide sequences (e.g., van der Waals, hydrogen bonding, and π–π stacking).
Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor
Authors: Fiordelisio Tatiana , Buendia-Roldan Ivette , Hautefeuille Mathieu , Del-Rio Diana , Ríos-López Diana G. , Zamarrón-Hernández Diego , Amat-Shapiro Samuel , Campa-Higareda Andrea , Jiménez-Díaz Edgar , González-Villa Erika , Nelson-Mora Janikua , García-Carreño Natllely , López-Aparicio Jehú , Montes Eduardo , Santiago-Ruiz Armando , Pardo Annie , Selman Moisés
Published: June 2021

In spite of a current increasing trend in the development of miniaturized, standalone point-of-care (PoC) biosensing platforms in the literature, the actual implementation of such systems in the field is far from being a reality although deeply needed. In the particular case of the population screenings for local or regional diseases related to specific pathogens, the diagnosis of the presence of specific antibodies could drastically modify therapies and even the organization of public policies. The aim of this work was to develop a fast, cost-effective detection method based on the manipulation of functionalized magnetic beads for an efficient diagnosis of hypersensitivity pneumonitis (HP), looking for the presence of anti-pigeon antigen antibodies (APAA) in a patient’s serum. We presented a Diagnostic Biosensor Method (DBM) in detail, with validation by comparison with a traditional high-throughput platform (ELISA assay). We also demonstrated that it was compatible with a microfluidic chip that could be eventually incorporated into a PoC for easy and broad deployment using portable optical detectors. After standardization of the different reaction steps, we constructed and validated a plastic chip that could easily be scaled to high-volume manufacturing in the future. The solution proved comparable to conventional ELISA assays traditionally performed by the clinicians in their laboratory and should be compatible with other antibody detection directly from patient samples.
Trends in the Design of Intensity-Based Optical Fiber Biosensors (2010–2020)
Authors: De Acha Nerea , Socorro-Leránoz Abián B. , Elosúa César , Matías Ignacio R.
Published: June 2021

There exists an increasing interest in monitoring low concentrations of biochemical species, as they allow the early-stage detection of illnesses or the monitoring of the environment quality. Thus, both companies and research groups are focused on the development of accurate, fast and highly sensitive biosensors. Optical fiber sensors have been widely employed for these purposes because they provide several advantages for their use in point-of-care and real-time applications. In particular, this review is focused on optical fiber biosensors based on luminescence and absorption. Apart from the key parameters that determine the performance of a sensor (limit of detection, sensibility, cross-sensibility, etc.), other features are analyzed, such as the optical fiber dimensions, the sensing set ups and the fiber functionalization. The aim of this review is to have a comprehensive insight of the different aspects that must be taken into account when working with this kind of sensors.
A Life Cycle Cost Analysis of Structural Insulated Panels for Residential Buildings in a Hot and Arid Climate
Authors: Dhaif Muataz , Stephan André
Published: June 2021

In hot and humid climatic conditions, cooling tends to dominate building thermal energy use. Cooling loads can be reduced through the adoption of efficient building envelope materials, such as Structural Insulated Panels (SIPs). This study quantifies the life cycle cost and operational energy of a representative case-study house in Bahrain using SIPs and hollow concrete blocks (HCBs) for the envelope over a period of 50 years. Operational energy is calculated using a dynamic energy simulation tool, operational costs are calculated based on the energy demand and local tariff rates, and construction costs are estimated using market prices and quotations. The life cycle cost is quantified using the Net Present Cost technique. Results show that SIPs yield a 20.6% reduction in cooling energy use compared to HCBs. For SIP costs of 12 and 17 USD/m2, the SIP house was cheaper throughout, or had a higher capital cost than the HCB house (breaking even in year 33), respectively. We propose policy recommendations with respect to material pricing, electricity tariffs, and energy efficiency, to improve the operational energy efficiency of houses in Bahrain and similar countries along the Arabian Peninsula.
Increased Tumor Growth Rate and Mesenchymal Properties of NSCLC-Patient-Derived Xenograft Models during Serial Transplantation
Authors: Pardo-Sánchez José Miguel , Mancheño Nuria , Cerón José , Jordá Carlos , Ansotegui Emilio , Juan Óscar , Palanca Sarai , Cremades Antonio , Gandía Carolina , Farràs Rosa
Published: June 2021

Non-small-cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. The high mortality is very often a consequence of its late diagnosis when the cancer is already locally advanced or has disseminated. Advances in the study of NSCLC tumors have been achieved by using in vivo models, such as patient-derived xenografts. Apart from drug screening, this approach may also be useful for study of the biology of the tumors. In the present study, surgically resected primary lung cancer samples (n = 33) were implanted in immunodeficient mice, and nine were engrafted successfully, including seven adenocarcinomas, one squamous-cell carcinoma, and one large-cell carcinoma. ADC tumors bearing the KRAS-G12C mutation were the most frequently engrafted in our PDX collection. Protein expression of vimentin, ezrin, and Ki67 were evaluated in NSCLC primary tumors and during serial transplantation by immunohistochemistry, using H-score. Our data indicated a more suitable environment for solid adenocarcinoma, compared to other lung tumor subtypes, to grow and preserve its architecture in mice, and a correlation between higher vimentin and ezrin expression in solid adenocarcinomas. A correlation between high vimentin expression and lung adenocarcinoma tumors bearing KRAS-G12C mutation was also observed. In addition, tumor evolution towards more proliferative and mesenchymal phenotypes was already observed in early PDX tumor passages. These PDX models provide a valuable platform for biomarker discovery and drug screening against tumor growth and EMT for lung cancer translational research.
Differential Expression of BOC, SPOCK2, and GJD3 Is Associated with Brain Metastasis of ER-Negative Breast Cancers
Authors: Pedrosa Rute M. S. M. , Wismans Leonoor V. , Sinke Renata , van der Weiden Marcel , van Eijck Casper H. J. , Kros Johan M. , Mustafa Dana A. M.
Published: June 2021

Background: Brain metastasis is considered one of the major causes of mortality in breast cancer patients. To invade the brain, tumor cells need to pass the blood-brain barrier by mechanisms that are partially understood. In primary ER-negative breast cancers that developed brain metastases, we found that some of the differentially expressed genes play roles in the T cell response. The present study aimed to identify genes involved in the formation of brain metastasis independently from the T cell response. Method: Previously profiled primary breast cancer samples were reanalyzed. Genes that were found to be differentially expressed were confirmed by RT-PCR and by immunohistochemistry using an independent cohort of samples. Results: BOC, SPOCK2, and GJD3 were overexpressed in the primary breast tumors that developed brain metastasis. BOC expression was successfully validated at the protein level. SPOCK2 was validated at both mRNA and protein levels. SPOCK2 and GJD3 mRNA overexpression were also found to be associated with cerebral metastasis in an external online database consisting of 204 primary breast cancers. Conclusion: The overexpression of BOC, SPOCK2, and GJD3 is associated with the invasion of breast cancer into the brain. Further studies to determine their specific function and potential value as brain metastasis biomarkers are required.
Advances in Chemokine Signaling Pathways as Therapeutic Targets in Glioblastoma
Authors: Urbantat Ruth , Vajkoczy Peter , Brandenburg Susan
Published: June 2021

With a median patient survival of 15 months, glioblastoma (GBM) is still one of the deadliest malign tumors. Despite immense efforts, therapeutic regimens fail to prolong GBM patient overall survival due to various resistance mechanisms. Chemokine signaling as part of the tumor microenvironment plays a key role in gliomagenesis, proliferation, neovascularization, metastasis and tumor progression. In this review, we aimed to investigate novel therapeutic approaches targeting various chemokine axes, including CXCR2/CXCL2/IL-8, CXCR3/CXCL4/CXCL9/CXCL10, CXCR4/CXCR7/CXCL12, CXCR6/CXCL16, CCR2/CCL2, CCR5/CCL5 and CX3CR1/CX3CL1 in preclinical and clinical studies of GBM. We reviewed targeted therapies as single therapies, in combination with the standard of care, with antiangiogenic treatment as well as immunotherapy. We found that there are many antagonist-, antibody-, cell- and vaccine-based therapeutic approaches in preclinical and clinical studies. Furthermore, targeted therapies exerted their highest efficacy in combination with other established therapeutic applications. The novel chemokine-targeting therapies have mainly been examined in preclinical models. However, clinical applications are auspicious. Thus, it is crucial to broadly investigate the recently developed preclinical approaches. Promising preclinical applications should then be investigated in clinical studies to create new therapeutic regimens and to overcome therapy resistance to GBM treatment.