bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Frailty In Elderly Patients Attending A Multidisciplinary Wound Care Center: A Cohort Study.
Authors: Espaulella-Ferrer Mariona , Espaulella-Panicot Joan , Noell-Boix Rosa , Casals-Zorita Marta , Ferrer-Sola Marta , Puigoriol-Juvanteny Emma , Cullell-Dalmau Marta , Otero-Viñas Marta
Published: August 2021

Background: The incidence of frailty and chronic wounds increases with patients’ age. Knowledge of the relationship between frailty and wound healing progress is greatly lacking. Methods: The aim of this study is to characterize the degree of frailty in elderly patients attending a multidisciplinary wound care centers (MWCC). Additionally, we seek to assess the impact of frailty on the wound healing rate and wound healing time. An open cohort study was conducted on 51 consecutive patients aged > 70 years treated for wounds at an MWCC of an intermediate care hospital. The frailty score was determined according to the Frail-VIG index. Data were collected through patient questionnaires at the beginning of the study, and at six months or upon wound healing. Wounds were followed up every two weeks. To analyze the relationship between two variables was used the Chi-square test and Student’s or the ANOVA model. The t-test for paired data was used to analyze the evolution of the frailty index during follow-up.Results: A total of 51 consecutive patients were included (aged 81.1 ± 6.1 years). Frailty prevalence was 74.5% according to the Frail-VIG index (47.1% mildly frail, 19.6% moderately frail, and 7.8% severely frail). Wounds healed in 69.6% of cases at six months. The frailty index (FI) was higher in patients with non-healing wounds in comparison with patients with healing wounds (IF 0.31 ± 0.15 vs IF 0.24 ± 0.11, p=0.043). A strong correlation between FI and wound healing results was observed in patients with non-venous ulcers (FI 0.37 ± 0.13 vs FI 0.27 ± 0.10, p=0.015). However, no correlation was observed in patients with venous ulcers (FI 0.17 ± 0.09 vs FI 0.19 ± 0.09, p=0.637). Wound healing rate is statically significantly higher in non-frail patients (3,26% wound reduction/day, P25-P75 0.8-8.8%/day) in comparison with frail patients (8.9% wound reduction/day, P25-P75 3.34-18.3%/day; p=0.044). Conclusion: Frailty is prevalent in elderly patients treated at an MWCC. Frailty degree is correlated with wound healing results and wound healing time.
Targeted Inhibition of ROCK Specifically in the Polarization Interstitial Macrophages Suppresses Pulmonary Fibrosis
Authors: Li Qingfang , Cheng Yuan , Bi Zhenfei , Ma Xuelei , Wei Yuquan , Wei Xiawei
Published: August 2021

Background Pulmonary fibrosis (PF) is a kind of progressive interstitial lung disease with no effective therapy. Rho/ROCK pathway has been confirmed to be activated in the process of PF in bleomycin-induced mice model in previous studies, which is involved in cell proliferation, tissue repair and regeneration. Bleomycin-induced and radiation-induced pulmonary fibrosis mice models were used to explore the effects and mechanism of WXWHO265, a novel unselected ROCK inhibitor, in PF.Methods The bleomycin-induced mice models were constructed by intratracheal instillation. The radiation-induced mice model were established by bilateral thoracic irradiation. Intragastric administration was applied to the WXWHO265 treated groups one day after model established. Flow cytometry, qRT-PCR, HE staining, Masson staining, and immunohistochemistry (IHC) analysis were used for further investigation.ResultsIn both types of PF models, the fibrosis in the lung was severe and became worse as time progressed. The status of mice in WXWHO265 treated groups was better than control group (saline treated group). The proportion of M2 macrophages in the lung tissue of bleomycin-induced group significantly increased compared to control group. The proportion of M2 macrophage of day 28 was higher than day 14 and day 7 in both PF models. The proportions of M2 macrophage in WXWHO265 high dose group(25mg/kg) and control group were statistically different (p<0.05). The p-STAT3 in lung tissue was significantly decreased in the day 28 in PF models. In vitro macrophages polarization experiment, the macrophages transformed into M2 macrophages by IL-4 stimulating. The proportion of M2 macrophages decreased after treated with WXWHO265. ConclusionsInhibiting ROCK could significant ameliorate PF in mice model by regulating the polarization of interstitial macrophages. Furthermore, the results showed that ROCK regulated the polarization of M2 macrophages by mediating the phosphorylation of STAT3, which might be a potential target in treating PF.
NSUN2-Mediated Splice Site m5C Methylation Modification Promotes the Progression of Colon Cancer Through Modulating the Discrepant Cleavage of tRNA-Arg
Authors: Luan Na , Cao Yanjing , Sun Jianguo , Mu Jiayi , Mu Yali , Ye Xun , Zhou Qin , Xu Miaorong , Tang Zhe , Wang Jianwei
Published: August 2021

Background: Hypoxia is a key driving factor for the tumour microenvironment restructuring, which leading to the variation of gene expression profiling in cancer cells. Increasing evidence reveals the initial action of hypoxia in the epitranscriptomics including RNA methylation. The role of tRNA-derived fragments (tRFs) in regulating tumour metastasis potential has attracted attention. Methods: The expression of tRFs in colon cancer cells under hypoxia were evaluated based on full-transcript sequencing and bioinformatics analysis and their effects on colon cancer metastasis were detected by transwell assays. The role of C34 was verified by introducing mutation and artificial m5C modification. The effects of NSUN2 on the biological characteristics of colon cancer cells were investigated on the basis of gain-of-function and loss-of function analyses. Lung metastasis model further uncovered the roles of NSUN2 and key tRF in tumour progression. Assays of RNA immunoprecipitation-qPCR (RIP-qPCR) were performed to identify that NSUN2 is a key methyltransferase for cysteine modification at C34 of tRNA-Arg.Results: The present study verified the up-expression of tRF (tRF-20-MEJB5Y13) and down-expression of tRFs (tRF-20-M0NK5Y93 and tRF-21-3OPP6N7KE) in colon cancer cells under hypoxia, and all of them were derived from different tRNA-Arg. Contradictory effects of these three tRNA-Arg-derived tRFs on metastatic potential of colon cancer were demonstrated in this study. The sequence differences and the key nucleotide bases of tRNA with the methylation modification potential among the source tRNAs were analysed. Notably, our data identified C34 of tRNA-Arg as a key site that may play an important role in hypoxia-mediated tRNA-Arg discrepant cleavage. We further investigated that NSUN2 mediated specific site methylation of tRNA-Arg at C34, thereby protecting tRNA from cleavage by endonuclease and subsequently promoting the colon cancer metastasis both in vitro and in vivo. Conclusion: The present study elaborates on the precise regulatory mechanism of m5C methylation and the role in the selective cleavage of tRNA from the perspective of noncoding RNA methylation epitranscriptomics, providing a novel insight into the molecular basis of selective expression of tRFs in colon cancer under hypoxia.
The Efficacy of Earplugs at a Major Hazard Facility
Authors: Lee Kah Heng , Benke Geza , McKenzie Dean
Published: August 2021

Introduction:At a major hazard facility, sporadic cases of occupational noise-induced hearing loss have occurred despite the use of hearing protection devices. Preliminary testing with a system for measuring personal attenuation ratings (PAR) suggested that earplugs may not achieve the attenuation implied by their Australian Class designation in field-use conditions. We believe that ours to be a world-first study at the time it was conducted to check the relationship between Personal Attenuation Rating (PAR) and the Australian SLC80 classification for hearing protection devices, and the findings can be used to complement studies based on the American Noise Reduction Ratio (NRR).Methods:A cross-sectional study was performed with 65 volunteers. Participants were recruited from Operations and Maintenance Technicians at the major hazard facility. The participants had their PARs checked with different earplug types using the 3MTM E-A-RfitTM system. An earplug is deemed to have achieved a ‘pass’ if it achieved 22 dB attenuation for 80% of users (the definition for Australian Class 4 hearing protection)Results:None of the earplug types achieved the attenuation implied by their class rating when tested in field-use conditions. There were statistically significant differences in the frequency of achieving a ‘Pass’, and in PAR, depending on earplug type. Roll-down foam earplugs may provide superior attenuation compared to pre-moulded earplugs.Conclusion:Earplugs are unlikely to achieve the attenuation found in laboratory conditions during field-use. Personalised selection of hearing protection devices based on fit-testing results should be encouraged.
Study On The Effect of Bond Strength On The Failure Mode of Coarse-Grained Sandstone In Weakly Cemented Stratum
Authors: Yang Xianda , Sun Lihui , Song Jiale , Yang Bensheng , Lan Chengren
Published: August 2021

Bond strength is one of the most important parameters and can affect the macroscopic mechanical properties and the damage state of the rock to some degree. The coarse-grained sandstone with strength of less than 40 MPa was studied by the controlled variable method. The influence of parallel bond strength on the peak strength and failure mode of coarse-grained sandstone was simulated, the evolution law of peak strength and failure mode of bond strength were comprehensively analyzed. The results show that the peak strength of rock was positively correlated with the bond strength, the difference value between tensile and shear crack was negatively correlated with tensile bond strength and positively correlated with shear bond strength. Tensile-shear bond strength ratio less than 0.5, the peak strength of the rock was usually stable at the certain extreme value under a constant tensile bond strength. Tensile crack was negatively correlated with the tensile-shear bond strength ratio, shear crack was positively correlated with the tensile-shear bond strength ratio. The failure mode of coarse-grained sandstone is shear failure. The research results can be used to guide the ground control of other mine stopes or roadways with weak cementation lithology.
The Effect of Special Educational Assistance In Early Childhood Education And Care On Psycho-Social Difficulties In Elementary School Children
Authors: Biele Guido , Lekhal Ratib , Overgaard Kristin R. , Wang Mari Vaage , Brandlistuen Ragnhild Eek , Friis Svein , Zeiner Pål
Published: August 2021

Background: Three to seven percent of pre-schoolers have developmental problems or child psychiatric disorders. Randomized controlled trials (RCTs) indicate that interventions in early childhood education and care improve long-term outcomes of children from disadvantaged backgrounds. It is unknown if effects generalize beyond the well-structured context of RCTs and to children who may not have a disadvantaged background but have developmental problems or psychiatric disorders. Methods: We use data from the population-based Norwegian Mother, Father and Child Cohort Study, recruiting pregnant women from 1999 to 2009, with child follow-up from ages 6, 18, and 36 months to ages 5, 7, and 8 years. This sub-study included 2499 children with developmental problems or psychiatric disorders at age five. We investigate the effects of special educational assistance at age five on mother-reported internalizing, externalizing, and communication problems at age eight. We analyse bias due to treatment by indication with directed acyclic graphs, adjust for treatment predictors to reduce bias, and estimate effects in different patient groups and outcome domains with a hierarchical Bayesian model.Results: In the adjusted analysis, pre-schoolers with special educational assistance had on average by 0.1 (0.04-0.16) standardised mean deviation fewer psycho-social difficulties in elementary school. Mean effect sizes varied between groups and outcomes.Conclusion: We estimate positive effects of educational assistance during the transition from preschool to the school years. It should therefore be considered as an intervention for pre-schoolers with developmental or behaviour problems. More research with improved measurements of treatment and outcomes is needed to identify success factors for their implementation.
Synergistic Effect of Chloroquine and Copper to the Euryhaline Rotifer Proales Similis
Authors: Rebolledo Uriel Arreguin , Rico-Martínez Roberto , Fernández Rocío , Páez-Osuna Federico
Published: August 2021

Chloroquine (CQ) has been widely used for many years against malaria and various viral diseases. Its important use and high potential to being persistent make it of particular concern for ecotoxicological studies. Here, we evaluated the toxicity of CQ alone and in combination with copper (Cu) to the euryhaline rotifer Proales similis. All experiments were carried out using chronic toxicity reproductive five‐day tests and an application factor (AF) of 0.05, 0.1, 0.3 and 0.5 by multiplying the 24-h LC50 values of CQ (4250 µg/L) and Cu (68 µg/L). The rate of population increase (r, d-1) ranged from 0.50 to 52 (controls); 0.19 to 0.39 (CQ); 0.09 to 0.42 (Cu); and -0.03 to 0.29 (CQ-Cu) and decreased significantly as the concentration of both chemicals in the medium increased. Almost all tested mixtures induced synergistic effects, mainly as the AF increased. We found that the presence of Cu intensifies the vulnerability of organisms to CQ and vice versa. These results stress the potential hazard that these combined chemicals may have on the aquatic systems. This research suggests that P. similis is sensitive to CQ as other standardized zooplankton species and may serve as a potential test species in the risk assessment of emerging pollutants in marine environments.
Transcriptomic and metabolic analysis uncovers the role of light quality in carotenoid accumulation of grapefruit during ripening
Authors: Huang Liulian , Hu Linping , Kong Wenbin , Yang Can , Xi Wanpeng
Published: August 2021

Light, a crucial environmental signal, is involved in the regulation of secondary metabolites. To understand the mechanism by which light influences carotenoid metabolism, grapefruits were bagged with four types of light-transmitting bags that altered the transmission of solar light. We showed that light-transmitting bagging induced changes in carotenoid metabolism during fruit ripening. Compared with natural light, red light (RL)-transmittance treatments significantly increased the total carotenoid content by 142%. Based on weighted gene co-expression network analysis (WGCNA), ‘red’, ‘darkred’, ‘yellow’, ‘brown’ and ‘midnightblue’ modules were remarkably associated with carotenoid metabolism under different light treatment. Transcriptome analysis identified the transcription factors (TFs) bHLH74/91/122, NAC56/78/90/100, MYB/MYB308, WRKY7/55, MADS29/AGL61, ERF043/118 as being involved in the regulation of carotenoid metabolism in response to RL. Under RL treatment, these TFs regulated the accumulation of carotenoids by directly modulating the expression of carotenogenic genes, including PSY, Z-ISO2, ZDS6, LCYB, LCYE, CHYB, CCD1-1/1-3, CCD4-2 and NCED2/3. Based on these results, a network of the regulation of carotenoid metabolism by light in citrus fruits was preliminarily proposed.
These results showed that RL treatments have great potential to improve coloration and nutritional quality of citrus fruits.
Increase in Lower Limb Strength After Multimodal Pain Therapy in Patients With Low Back Pain
Authors: Kaiser Moritz , Brambrink Sara , Benditz Achim , Achenbach Leonard , Gehentges Matthias , König Matthias A
Published: August 2021

Study DesignProspective Study DesignObjectivesThe aim of the present study was to evaluate the efficacy of a multimodal pain therapy (MPM) regarding the objective parameter muscle strength of segment-dependent lower limb muscle groups before and after such a treatment. Methods52 patients with a history of low back pain and/or leg pain received standardized multimodal pain management. Strength of segment indicating lower limb muscles were assessed for each patient before and after ten days of treatment by handheld dynamometry. ResultsOverall strength increased significantly from 23.6kg ± 6.6 prior to treatment to 25.4 ± 7.3 after treatment, p≤0.001. All muscle groups significantly increased in strength with exception of great toe extensors. ConclusionDespite lower basic strength values at the beginning of treatment, all investigated muscle groups, with the exception of the great toe extensors, showed a significant increase of overall strength after completion of the multimodal pain management concept. Increased overall strength could help with avoiding further need of medical care by supporting patients’ autonomy in daily life activities, as well as maintaining working abilities. Thus, our study is the first to show a significant positive influence on lower limb strength in patients with low back pain after a conservative MPM program.
The Predictive Research of Dynamic Milling Force Based on Data Mining
Authors: Jin Lan , Zeng Xinlei , Lu Shiqi , Xie Liming , Zhang Xuefeng
Published: August 2021

In this paper, surface accuracy of the work-piece was improved by mining large amounts of machining data and obtaining potentially valuable information. By using data mining technology, a dynamic milling force prediction model has been established to keep with its working. The model was developed by a combination of Regression Analysis and RBF Neural Network. The internal relation of the data were analyzed in this study, such as milling force, cutting parameters, temperature, vibration and surface quality et.al, and the methods of Cluster Analysis and Correlation Analysis was used to extract and induct dynamic milling force variations on the effects with different situations. The results suggest that the proposed dynamic milling force model had a better prediction effect, which ensure production quality, reduce the occurrence of chatter and provide a more accurate basis for selecting process parameters.
Peri-Operative Cerebral Regional Oxygen Saturation Variability and Short-Term Outcomes Following Cardiac Surgery in Neonates
Authors: Abubakar Maryam O. , Zanelli Santina A. , Spaeder Michael C.
Published: August 2021

Decreased post-operative cerebral region oxygenation saturation (crSO2) variability, a surrogate for cerebral autoregulation, correlates with poor neurodevelopmental outcomes in neonates who undergo cardiac surgery. The goal of this study is to investigate the relationship between pre- and post-operative crSO2 variability in neonates requiring neonatal cardiac surgery for congenital heart disease (CHD). The variability of averaged 1-min crSO2 values was calculated for a minimum of 12h before and for the first 48h following cardiac surgery with cardiopulmonary by-pass in neonates between November 2019 and May 2021. The crSO2 variability increased by 9% with each additional postnatal day in the pre-operative monitoring period (p=0.009). There was a 40% decrease in crSO2 variability between the pre-and post-operative monitoring periods (p<0.001). There were no associations between the degree of decrease in crSO2 variability and CHD classification (aortic arch obstruction or single ventricle physiology). The crSO2 variability improves with each additional postnatal day but then decreases by almost half following cardiac surgery in neonates. We did not observe any association between pre-operative crSO2 variability and post-operative ventilator-free days, post-operative ICU days, or mortality.The long-term effects or significance of reduced crSO2 require further exploration.
Tandem repeats ubiquitously flank and select translation initiation sites.
Authors: Maddi Ali , Kavousi Kaveh , Arabfard Masoud , Ohadi Hamid , Ohadi Mina
Published: August 2021

Findings in yeast and human suggest that evolutionary divergence in cis-regulatory sequences impact translation initiation sites (TISs). Here we employed the TIS homology concept to study a possible link between all categories of tandem repeats (TRs) and TIS selection. Human and 83 other species were selected, and data was extracted on the entire protein-coding genes (n = 1,611,368) and transcripts (n = 2,730,515) annotated for those species from Ensembl 102. On average, every transcript was flanked by 1.19 TRs of various categories in their 120 bp upstream RNA sequence. We detected statistically significant excess of non-homologous TISs co-occurring with human-specific TRs, and vice versa. We conclude that TRs are abundant cis elements in the upstream sequences of TISs across species, and there is a link between all categories of TRs and TIS selection. TR-induced symmetric and stem-loop structures may function as genetic marks for TIS selection.
Nitrous Oxide Emission from a Flooded Tropical Wetland across a Vegetation and Land Use Gradient
Authors: Were David , Hein Thomas
Published: August 2021

Whereas wetland ecosystems are among the most vital natural carbon sinks, they are also important sources of nitrous oxide (N2O), a highly potent greenhouse gas. However, due to differences in wetland characteristics, N2O emission is likely to vary across wetland types. We investigated the: 1) influence of vegetation community (Typha latifolia; Typha, Phragmites mauritianus; Phragmites and Cyperus papyrus; Papyrus) in a natural tropical freshwater wetland, and 2) impact of converting a natural tropical freshwater wetland into a rice paddy wetland on N2O emission. Results showed that N2O emission (µg m− 2 h− 1) from the natural wetland did not vary significantly (p > 0.05) among the vegetation communities during both the dry and wet seasons (Typha = 0.6 ± 1.6 [SE] and 0.5 ± 1.4, Phragmites = 0.5 ± 1.7 and 0.4 ± 1.5, Papyrus = 0.5 ± 1.3 and 0.5 ± 1.5, respectively). These emission rates insignificantly differed (p > 0.05) from those recorded in the rice paddy wetland (dry season = 0.7 ± 2.8 and wet season = 0.6 ± 2.7). There was no significant correlation (p > 0.05) between soil physico-chemical characteristics and N2O emission. We concluded that vegetation community does not affect N2O emission from a natural tropical freshwater wetland under continuous flooding. Similarly, under continuous flooding and no fertilization conditions, converting a natural tropical freshwater wetland into a rice paddy wetland does not influence N2O emission. We roughly estimated total annual N2O emissions (T yr− 1) and their carbon dioxide equivalents (CO2e; T yr− 1) from all Uganda’s natural and rice paddy wetlands as: natural wetlands = 115.1 ± 342.8 (CO2e = 30,501.5 ± 90,842) and rice paddy wetlands = 0.9 ± 2.7 (CO2e = 242.5 ± 707.6).
A Micro-Scaled Graphene Based Tree-Shaped Wideband Printed MIMO Antenna For Terahertz Applications
Authors: Babu K. Vasu , Das Sudipta , Varshney Gaurav , S Gorre Naga Jyothi , Madhav Boddapati Taraka Phani
Published: August 2021

A tree-shaped graphene based microstrip MIMO antenna for terahertz applications is proposed. The proposed MIMO antenna is designed on a 600×300 µm2 polyimide substrate. The designed MIMO antenna exhibits a wide impedance bandwidth of 88.14% (0.276–0.711 THz) due to the suggested modifications in the antenna configuration. The MIMO design parameters like total active reflection coefficient (TARC), mean effective gain (MEG), envelope correlation coefficient (ECC) and diversity gain (DG), Channel capacity loss (CCL) are evaluated and their values are found within acceptable limits. The proposed MIMO structure offers MEG ≤ -3.0 dB, TARC≤ -10.0 dB, DG≈ 10 dB, CCL < 0.5 bps/Hz/sec and ECC < 0.01 at the resonant frequency. At the resonant frequency, the isolation between the radiating elements of the proposed MIMO is recorded as -52 dB. The variations in operating frequency and S-parameters are also analyzed as a function of the chemical potential (µc) of the graphene material. The parametric analysis, structural design evolution steps, surface current distribution, antenna characteristics parameters and diversity parameters are discussed in detail in this paper. The designed MIMO antenna is suitable for high speed short distance communication, video rate imaging, biomedical imaging, sensing, and security scanning in the THz frequency band.
Experimental and Numerical Study on the Flow Ripple of Circular-arc Gear Pumps Considering the Center Distance Deviation
Authors: Wei Xiaoling , Feng Yongbao , He Zhenxin , Liu Ke
Published: August 2021

Novel circular-arc gear pumps effectively solve the problems of oil trapping and flow pulsation experienced with traditional gear pumps. However, the center distance deviation associated with assembly and installation during gear pump processing has an important influence on the outlet pressure pulsation characteristics of circular-arc gear pumps. First, the circular-arc tooth profile equation, conjugate curve equation and meshing line equation were derived to design the circular-arc gear meshing and center distance deviation functions. Second, the circular-arc gear tooth profile was accurately obtained. Then, a pressure pulsation characteristic simulation model for the novel circular-arc gear pumps considering the center distance deviation was established. The results show that with the increase of center distance deviation, the outlet flow rate of the arc gear pump increases first and then decreases greatly. Moreover, the center distance deviation has little effect on the independent tooth cavity pressure. Finally, the proposed fluid dynamic model is used to simulate a commercial circular-arc gear pump, which was tested within this research for modeling validation purposes. The comparisons highlight the validity of the proposed simulation approach.
Circulating tumor cell methylation profiles reveal the classification and evolution of non-small cell lung cancer
Authors: Jiang Jia-Hao , Chen Chang-Yue , Gao Jian , Li Jing , Lu Yuan , Fang Wang , Wang Hai-Kun , Yuan Yun-Feng , Ding Jian-Yong
Published: August 2021

BackgroundsThe ability of circulating tumor cells (CTCs) to identify lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) holds great promise for improving pathological diagnosis and selecting treatment in non-small cell lung cancer (NSCLC). In addition, previous studies have shown that DNA methylation exhibits cell and tissue specificity. Thus, we aimed to explore the methylation status of CTCs in LUAD and LUSC and identify the potential biomarkers.Methodswe first analyzed Infinium 450K methylation profiles obtained from The Cancer Genome Atlas and Gene Expression Omnibus and further identified the results by performing whole-genome sequencing of CTCs in tumor and matched normal lung tissues and white blood cells from 6 NSCLC patients.Resultsthe bioinformatic analysis revealed that an NSCLC-specific DNA methylation marker panel which could accurately distinguish between LUAD and LUSC with high diagnostic accuracy. The whole-genome sequencing of CTCs in NSCLC patients also showed 100% accuracy for distinguishing between LUAD and LUSC based on CTC methylation profiles. To investigate the function of CTCs, we further analyzed similar and different methylation profiles between CTCs and their primary tumors and found very high similarities between CTCs and their primary tumor tissues, indicating that these cells inherit information from primary tumors. CTCs also showed some characteristics that were different from those of the primary tumor tissues, suggesting that CTCs evolve some unique characteristics after migrating from the primary tumor; these characteristics may be one of the reasons for the ability of tumor cells to evade immune surveillance.Conclusionthus, our study provides insight into the potential use of CTCs in pathological classification of NSCLC patients as well as CTC primary tumor inheritance and CTC evolution influence metastasis and immune escape.
MiR-615 Agomir Encapsulated in Pluronic F-127 Alleviates Neuron Damage and Facilitates Function Recovery after Brachial Plexus Avulsion
Authors: Chen Kangzhen , Ding Lu , Shui Hua , Liang Yinru , Zhang Xiaomin , Wang Tao , Li Linke , Liu Shuxian , Wu Hongfu
Published: August 2021

Brachial plexus avulsion(BPA)is a devastating traumatic peripheral nerve injury complicated with paralysis of the upper extremity. We previously reported that leucine-rich repeat and immunoglobulin-like domain-containing NOGO receptor-interacting protein 1 (LINGO-1) has a potent role in inhibiting neuron survival and axonal regeneration after central nervous system (CNS) damage and miR-615 is a potential microRNA (miRNA) negatively regulated LINGO-1. However, the effect of miR-615 in BPA remains to be elucidated. Accumulating evidence indicates that pluronic F-127 (PF-127) hydrogel could serve as a promising vehicle for miRNA encapsulation. Thus, to further explore the potential role of hydrogel-miR-615 in BPA-reimplantation, the present study established the BPA rat model and injected miR-615 agomir encapsulated by PF-127 hydrogel into the reimplantation site using a microsyringe. In this study, results indicated that hydrogel-miR-615 agomir effectively alleviated motoneuron loss by LINGO-1 inhibition, promoted musculocutaneous nerve regeneration and myelination, reduced astrocytes activation, promoted angiogenesis and attenuated peripheral amyotrophy, leading to improved motor functional rehabilitation of the upper extremity. In conclusion, our findings demonstrate that miR-615-loaded PF-127 hydrogel may represent a novel therapeutic strategy for BPA treatment.
Incidence and Risk Factor of Allergic Contact Dermatitis to 2-Octyl Cyanoacrylate and n-Butyl Cyanoacrylate Topical Skin Adhesives
Authors: Park Young Hwan , Choi Jeong Seok , Choi Jung Woo , Kim Hak Jun
Published: August 2021

Although the use of topical skin adhesives has increased as an alternative to conventional skin closure methods, studies on the incidence and risk factors of allergic contact dermatitis (ACD) to topical skin adhesives have been limited. The purpose of this study was to investigate the incidence and risk factors of ACD after the use of 2-octyl cyanoacrylate and n-butyl cyanoacrylate topical skin adhesives. We retrospectively reviewed 1145 patients (739 patients with 2-octyl cyanoacrylate and 406 patients with n-butyl cyanoacrylate) who underwent skin closure with topical skin adhesives. Variables suspected to correlate with ACD were retrieved from medical records and analyzed to determine risk factors. The incidence of ACD from the use of 2-octyl cyanoacrylate and n-butyl cyanoacrylate topical skin adhesives was 2.7% and 2.2%, respectively. There was no statistically significant difference in the incidence between the two ingredients. In logistic regression analysis, none of the variables were found to increase the risk of ACD in both 2-octyl cyanoacrylate and n-butyl cyanoacrylate topical skin adhesives. As ACD occurs without risk factors in 2–3% of patients who used 2-octyl cyanoacrylate or n-butyl cyanoacrylate topical skin adhesives, clinicians and patients should be aware of these facts before using topical skin adhesives.
Design and Development of a Commercial Hair Trimmer Cleaner Unit
Authors: Kumar Ganesh Raj , Khan Adam , Rajesh S , Khan Shahul Hamid
Published: August 2021

A product design and development process has been adopted to study and improve the performance of unit cleaner system. A hair trimmer with liquid detergent in cartridge is fixed as a cleaner system to design and analyse. The 3D model of the system is designed with NX CAD software. The modification is cartridge unit is made on counter face to increase the turbulence of the fluid in the wash zone. The file has been extracted and imported in LS Dyna for meshing the model for detergent liquid flow analysis. The velocity vector and pressure magnitude of the fluid flow is analysed through a StarCCM+ higher fluid analysis software. The input process condition of the pump in cartridge assemble unit has a speed 2000 rpm and velocity of the liquid as 1 m/s. From the analysis, the suction pressure noticed at the pump zone is 950 Pa and the discharge of fluid in the pump is 850 – 900 Pa at wash zone. Difference in fluid velocity found decreased and it is in the range of 0.8 to 0.95 m/s. The predominant factor in the design is alternate openings in case baffle has increased the turbulence in pressure and the velocity of the fluid found same.
Global Burden of Sexually Transmitted Infections: 1990 - 2019
Authors: Huang Jiaofeng , Lin Su , Pan Jinshui , Lu Lingling , Liu Bang , Zheng Jiaolong , Wu Haicong , Zeng Zhiyu , Li Dongliang
Published: August 2021

BackgroundSexually transmitted infections (STIs) are major public health problems worldwide. Understanding the disease burden are crucial for health policy making. This study was to assess global and regional STIs incidence, mortality and disability‐adjusted life years (DALYs) from 1990 to 2019.Methods­­­­Data were extracted from the Global Burden of Disease study 2019, which is an open database for download. Age-standardized rate and estimated annual percentage changes (EAPC) were calculated to evaluate the burden of STIs over time.ResultsIn 2019, the total number of incident cases of STIs was 769.85 million worldwide. The age-standardized incidence rate was stable from 1990 to 2019 with the EAPC of −0.04 (95% UI: −0.09 to 0.01). In 2019, the number of deaths caused by STIs was 89.89×103, which was 15.51% lower than that of 1990 (106.52×103). A decreasing trend from 1990 to 2019 was observed in the age-standardized death and DALYs. The age-standardized death and DALYs rate due to STIs were the highest in the younger age (<14 years old). As for different diseases, syphilis was the least common STIs with an age-standardized incidence rate of 178.48/100,000, while syphilis was also the main contributor to the age-standardized death and DALYs rate. These two indicators were negatively associated with sociodemographic level.ConclusionsThe global incidence of STIs has been persistently high from 1990 to 2019, while the age-standardized death and DALYs rate has decreased recently. More attention should be paid to the younger population, patients with syphilis and regions with low sociodemographic index.
Mechanism of Quercetin Therapeutic Targets for Alzheimer Disease and type 2 Diabetes Mellitus
Authors: Zu Guoxiu , Sun Keyun , Li Ling , Zu Xiuli , Han Tao , Huang Hailiang
Published: August 2021

Quercetin has demonstrated antioxidant, anti-inflammatory, hypoglycemic, and hypolipidemic activities, suggesting therapeutic potential against type 2 diabetes mellitus (T2DM) and Alzheimer’s disease (AD). In this study, potential molecular targets of quercetin were first identified using the Swiss Target Prediction platform and pathogenic targets of T2DM and AD were identified using Online Mendelian Inheritance in Man (OMIM), DisGeNET, TTD, DrugBank, and GeneCards databases. The 95 targets shared among quercetin, T2DM, and AD were used to establish a protein–protein interaction (PPI) network, top 25 core genes, and protein functional modules using MCODE. Metascape was then used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. A protein functional module with best score was obtained from the PPI network using CytoHubba, and 6 high-probability quercetin targets (AKT1, JUN, MAPK, TNF, VEGFA, and EGFR) were confirmed by docking simulations. KEGG pathway enrichment analysis suggested that the major shared mechanisms for T2DM and AD include “AGE-RAGE signaling pathway in diabetic complications,” “pathways in cancer,” and “MAPK signaling pathway” (the key pathway). We speculate that quercetin may have therapeutic applications in T2DM and AD by targeting MAPK signaling, providing a theoretical foundation for future clinical research.
Principles of design optimization for springs
Authors: V. Kobelev
Published: August 2021

The spring is the widespread resilient element which is used in the industrial machinery and automotive systems, as diesel fuel pumps, valvetrains, brakes, suspensions, seats, doors and control elements. For reducing impact events in some heavy trucks and railroad cars primarily, helical, or coil, springs are applied. In some vehicles torsion bars are used instead of the coil springs. The reduction of weight of the suspension springs causes the decrease of unsprung mass of the axle and has a positive influence on the comfort, traction and steering properties of the car. The development of modern passenger cars has highlighted a trend towards reduced package space for suspension components in order to maximize package space for occupants and loads. Such requirements lead to reduction in spring dimensions and wire cross-section. Springs can be found in high-precision testing devices, where springs play the role of energy harvesters. The efficient design procedures for spring elements are based on the modern simulation and optimization methods. The design formulas for linear helical springs with an inconstant wire diameter and with a variable mean diameter of spring are presented. Based on these formulas the optimization of spring for given spring rate and strength of the wire is per-formed.
DELAYED HEMOLYTIC TRANSFUSION REACTIONS ( DHTR) AND HYPERHEMOLYSIS SYNDROME IN CHILDREN WITH SICKLE CELL DISEASE (SCD ) : A CASE REPORT: JUBAIL, KINGDOM OF SAUDI ARABIA
Authors:   Consultant Pediatric Hematology Royal Commission Hospital , Jubail, Kingdom Of Saudi Arabia., Shalaby Khaled , Faisal Ahmed AL Muaibid Abdullah ,   Medical Intern, Baha University, Baha, Kingdom Of Saudi Arabia., Jehad Abousada Hoda ,   Obestetric & Gynecology physician, KAMC , Jeddah , Kingdom of Saudi Arabia., Mwase Meridah ,   Medical Technologist ( MT) - Blood Transfusion Technology Royal Commission Hospital , Jubail, Kingdom Of Saudi Arabia.
Published: August 2021

Delayed hemolytic transfusion reactions (DHTRs) occur in patients who have received transfusions in the past. These patients may have very low antibody titers that are undetectable on pretransfusion testing, so that seemingly compatible units of red blood cells (RBCs) are transfused. DHTRs are a potentially life-threatening complication of sickle-cell disease (SCD) treatment. In SCD, DHTRs appear to be an immune process that develop because of differences in erythrocyte antigens between blood donors and patients. Aim:DHTR is one of serious complication of blood transfusion that happens due to alloimmunized which is lead to hyperhaemolysis syndrome , DHTR can be managed by steroid and Intravenous immunoglobulin ( IVIG ) and prevented by accurate records with extended matching for certain population. Methodology: The consent of the patient and his family was obtained to publish and highlight his health condition in detail and to follow up with the case to match the research results and work on it under the framework of case reporting. Conclusion: DHTR can be managed by steroid and Intravenous immunoglobulin ( IVIG ) with good outcomes,DHTR can be prevented by means of accurate records, extended matching for certain population with high risk of developing alloantibodies such as individuals with SCD and B-thalassemia with proper handling of administration of blood product and proper notation in the patient medical records and blood bank records as well as the use of medical card for patient to known RBC alloantibodies.
Investigating the relationship between tax culture and tax communication in Kazakhstan
Authors: Kuandykov Nurzhan , Abil Yerlan , Utepov Arman , Nurkey Aisulu , Abenova Aisulu
Published: August 2021

The study demonstrated the level of tax culture as the basis for tax compliance. In turn, the formation of a strong tax culture depended on certain factors. Therefore, there were statements that the degree of such a culture depends on the level of tax communication. To show this connection, this paper analyzed the content of text arrays and products of communicative correspondence by keywords income and asset declaration in the Google search system (content analysis). Results of the analysis demonstrated more than 160 news articles and numerous social media posts. Moreover, the study contains the analytics of social media powered by YouScan artificial intelligence platform. This paper also presents the analysis and results of the sociological survey, in which more than a thousand people participated in the two biggest cities in Kazakhstan. The data of the survey were analyzed by the software Statistica 10 – the statistical data analysis system. The survey showed low tax awareness of citizens, almost 72% of respondents did not know about the introduction of the declaration of income and welfare. The results obviously demonstrated that there is a positive correlation between investigated issues, and poor utilization of information and communications technology by the government. In this way, this paper explored the relationship between tax culture and tax communication, where the awareness of citizens could be a huge step in the formation of honest taxpayers and increase confidence in government institutions.
DIFFERENCES IN SPATIAL ORIENTATION IN CHILDREN WITH AND WITHOUT VISUAL IMPAIRMENT WITH REGARD TO AGE, GENDER AND SCHOOL ACHIEVEMENT
Authors:   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina, Tulumović Ševala , Omerćehajić Dragica ,   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina, Husić-Đuzić Izeta ,   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina
Published: August 2021

The aim of the research is to examine the differences in the spatial orientation of children with and without visual impairment with regard to age, gender, and school achievement. The sample of respondents consisted of a total of 62 respondents, of whom: 31 respondents with visual impairment and 31 respondents without visual impairment, aged 7-11, both sexes obtained from the pupil population from the first to the eighth grade of regular primary schools in the Tuzla Canton area. The obtained results were processed by descriptive analysis, t-test and variance analysis. By analyzing the results on variables for the estimation of spatial orientation, we can conclude that the two investigated groups statistically significantly differ in spatial orientation with regard to: age, while the differences were not shown on the variables: gender, and school achievement. The results of the research can be used to create new methods for implementing and improving the rehabilitation process in this population of children. Rehabilitation with children with visual impairment can positively influence the reduction of the differences in the spatial orientation of this population in relation to children of intact vision, and on the educational process, social and professional integration of this population.
THE ROLE OF PERSONAL ASSISTANT IN YOUNG PEOPLE WITH DISABILITIES
Authors:   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina, Husić-Đuzić Izeta , Tulumović Ševala ,   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina
Published: August 2021

The aim of the paper is to examine the need for a personal assistant in young people with disabilities. The sample of respondents consisted of young people with disabilities, chronologically aged 18-35, both genders, 50 of them, selected by random selection method from the Tuzla Canton area. The data obtained by the research were processed using the method of parametric and nonparametric statistics. Frequencies, percentages and measures of central tendency are calculated (arithmetic mean, standard deviation). The results obtained showed that there is a need for a personal assistant for young people with disabilities.
PERCEPTION OF PARENTAL SUPPORT BY DEAF AND HARD-OF-HEARING STUDENTS
Authors:   Center for Hearing and Speech Rehabilitation, Sarajevo, Bosnia and Herzegovina, Povlakić Hadžiefendić Meliha , H. Mahmutović Esad ,   Center for Education and Rehabilitation of hearing and speaking Tuzla, Bosnia and Herzegovina, Hasanbegović Husnija ,   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina
Published: August 2021

The aim of the paper was to determine the perception of parental support and understanding by deaf and hard-of-hearing students. The sample consisted of 47 deaf and hard-of-hearing students, both genders, with an average age of 16 ± 1.27 years. Children's Perceptions of Parents Scale, (Grolnick, Ryan & Deci, 1991), which consists of three subscales, and which measure parental involvement, support of autonomy and parental warmth, especially for the mother, especially for the father was used in this research. The data were processed by descriptive analysis, and the t-test was used to test the mother's support perception difference in relation to father’s support perception. The results showed that deaf and hard-of-hearing students mostly positively perceived the parent involvement, support of autonomy and warmth of both parents, but a statistically significant difference in the individual perception of parents was found in favor of the mothers.
THE NATURE OF THE CHINESE SCRIPT
Authors:   Department of Oriental Studies, Faculty of Philology, Belgrade University, Serbia, Xiaolei Jin
Published: August 2021

This paper introduces the nature of the Chinese script, with the focus on its origin and development, the main four kinds of creation methods, the basic strokes of Chinese characters and the significance of its radical structure. The author believes that Chinese characters are the carriers of the script, phoneme and meaning as well as of the grammatical structure of the Chinese language. Hence, the Latin alphabet for the Chinese phonetic system cannot replace Chinese character, and learning Chinese character is very important for foreign learners. Finally, the author gives a mnemonic of the basic rules for stroke order that facilitates learners' memory and helps them write characters correctly and quickly.
THE IMPACT OF GEOMETRY KNOWLEDGE ON THE ORIENTATION AND MOBILITY OF BLIND STUDENTS
Authors:   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina, Teskeredžić Amela , Begić-Jahić Hurma ,   Faculty of Education and Rehabilitation, University of Tuzla, Bosnia and Herzegovina
Published: August 2021

The problem of blindness and other visual impairment is very important because it affects millions of people in the world. For those suffering from innate visual impairment, it is very difficult to imagine the world like it is, the fight begins from early school stages and continues until the end of life. This paper describes how the learning of basic geometric shapes, and their different positions, has an impact on the formation of a mental scheme in orientation and mobility. Geometry and its knowledge represent only one small part necessary for a safer and more independent movement of blind people. The research has shown that the quarterly individual work, on the adoption of geometric concepts and positions in students, is making progress in overcoming the recent problems. Spatial visualization implies understanding and imaginary movements, which, with spatial orientation and recognition of parts and relationships in the micro and macro environment, require mental rotation in relation to the position of the body. Therefore, it all together influences the formation of the mental scheme of the blind people, which is the basis for orientation and movement.