bluuebunny/crossref_metadata_2025 huggingface dataset:
Data from Omental Preadipocytes Stimulate Matrix Remodeling and IGF Signaling to Support Ovarian Cancer Metastasis
Authors: Waters Jennifer A. , Robinson Mikella , Lujano-Olazaba Omar , Lucht Cassidy , Gilbert Samuel F. , House Carrie D.
Published: July 2024

Abstract
Ovarian cancer can metastasize to the omentum, which is associated with a complex tumor microenvironment. Omental stromal cells facilitate ovarian cancer colonization by secreting cytokines and growth factors. An improved understanding of the tumor-supportive functions of specific cell populations in the omentum could identify strategies to prevent and treat ovarian cancer metastasis. Here, we showed that omental preadipocytes enhance the tumor initiation capacity of ovarian cancer cells. Secreted factors from preadipocytes supported cancer cell viability during nutrient and isolation stress and enabled prolonged proliferation. Coculturing with preadipocytes led to the upregulation of genes involved in extracellular matrix (ECM) organization, cellular response to stress, and regulation of insulin-like growth factor (IGF) signaling in ovarian cancer cells. IGF1 induced ECM genes and increased alternative NF-κB signaling by activating RelB. Inhibiting the IGF1 receptor initially increased tumor omental adhesion but decreased the growth of established preadipocyte-induced subcutaneous tumors as well as established intraperitoneal tumors. Together, this study shows that omental preadipocytes support ovarian cancer progression, which has implications for targeting metastasis.

Significance: Omental preadipocyte-mediated IGF1 signaling promotes ovarian cancer tumorigenesis and metastasis via extracellular matrix remodeling, revealing a role for preadipocytes in regulating ovarian cancer progression and highlighting potential therapeutic targets for metastatic disease.
Supplementary Figure S3 from Near-infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Patient-Derived Xenografts Using a Humanized Anti-Fibroblast Activation Protein Antibody
Authors: Kobayashi Teruki , Noma Kazuhiro , Nishimura Seitaro , Kato Takuya , Nishiwaki Noriyuki , Ohara Toshiaki , Kunitomo Tomoyoshi , Kawasaki Kento , Akai Masaaki , Komoto Satoshi , Kashima Hajime , Kikuchi Satoru , Tazawa Hiroshi , Shirakawa Yasuhiro , Choyke Peter L. , Kobayashi Hisataka , Fujiwara Toshiyoshi
Published: July 2024

Supplementary Figure S3
Supplementary File 1 from Inhibition of MALT1 and BCL2 Induces Synergistic Antitumor Activity in Models of B-Cell Lymphoma
Authors: Plotnik Joshua P. , Richardson Adam E. , Yang Haopeng , Rojas Estela , Bontcheva Velitchka , Dowell Colleen , Parsons Sydney , Wilson Ashley , Ravanmehr Vida , Will Christine , Jung Paul , Zhu Haizhong , Partha Sarathy Karunan , Panchal Sanjay C. , Mali Raghuveer Singh , Kohlhapp Frederick J. , McClure Ryan A. , Ramathal Cyril Y. , George Mariam D. , Jhala Manisha , Elsen Nathaniel L. , Qiu Wei , Judge Russell A. , Pan Chin , Mastracchio Anthony , Henderson Jared , Meulbroek Jonathan A. , Green Michael R. , Pappano William N.
Published: July 2024

Excel Workbook ABBV-MALT1 Pharmacology
Supplementary Figure 3 from Inhibition of MALT1 and BCL2 Induces Synergistic Antitumor Activity in Models of B-Cell Lymphoma
Authors: Plotnik Joshua P. , Richardson Adam E. , Yang Haopeng , Rojas Estela , Bontcheva Velitchka , Dowell Colleen , Parsons Sydney , Wilson Ashley , Ravanmehr Vida , Will Christine , Jung Paul , Zhu Haizhong , Partha Sarathy Karunan , Panchal Sanjay C. , Mali Raghuveer Singh , Kohlhapp Frederick J. , McClure Ryan A. , Ramathal Cyril Y. , George Mariam D. , Jhala Manisha , Elsen Nathaniel L. , Qiu Wei , Judge Russell A. , Pan Chin , Mastracchio Anthony , Henderson Jared , Meulbroek Jonathan A. , Green Michael R. , Pappano William N.
Published: July 2024

Efficacy of ABBV-MALT1 the OCI-LY10 CDX model
Supplementary Figure S3 from The Novel ATR Inhibitor M1774 Induces Replication Protein Overexpression and Broad Synergy with DNA-targeted Anticancer Drugs
Authors: Jo Ukhyun , Arakawa Yasuhiro , Zimmermann Astrid , Taniyama Daiki , Mizunuma Makito , Jenkins Lisa M. , Maity Tapan , Kumar Suresh , Zenke Frank T. , Takebe Naoko , Pommier Yves
Published: July 2024

This figure shows protein networks in SN-38/control and correlation plots between replication fork protection proteins and VE-822.
Supplementary Figure 5 from Ultrasensitive Response Explains the Benefit of Combination Chemotherapy Despite Drug Antagonism
Authors: Patterson Sarah C. , Pomeroy Amy E. , Palmer Adam C.
Published: July 2024

Supplemental Figure 5. Short-term adaptive resistance does not affect sequential treatments. MTA cell cultures were treated for 3 days with either 4-H-cyclophosphamide, doxorubicin, vincristine, or no treatment (control). Drugs were administered at concentrations producing 50% growth inhibition. Following treatment, cultures were washed of drug and recovered in drug-free media for 4 days. After recovery, dose responses were measured for 3-day treatments with 4-H-cyclophosphamide, doxorubicin, vincristine, prednisolone, and the CHOP combination. Figure 2a presents the results of these experiments with relative drug sensitivity quantified as ‘Area Over the Curve’ (AOC) (area between the dose-response curve and the line y=100% relative viability). Pre-treated cultures were not more resistant to chemotherapy than control cultures. Error bars represent 95% confidence intervals, n=8.
Supplementary Table S1 from Loss of the DNA Repair Gene RNase H2 Identifies a Unique Subset of DDR-Deficient Leiomyosarcomas
Authors: Nakazawa Michael S. , Silverman Ian M. , Rimkunas Victoria , Veloso Artur , Glodzik Dominik , Johnson Adrienne , Ohsumi Toshiro K. , Patel Shreyaskumar R. , Conley Anthony P. , Roland Christina L. , Soliman Pamela T. , Beird Hannah C. , Wu Chia-Chin , Ingram Davis R. , Lazcano Rossana , Song Dawon , Wani Khalida M. , Lazar Alexander J. , Yap Timothy A. , Wang Wei-Lien , Livingston J. Andrew
Published: July 2024

Anatomic location of ST-LMS and RNase H2 status. Absolute number and frequency of cases of RNase H2 loss in the ST-LMS cohort based on primary anatomic location.
Supplementary Table S7 from ETS1, a Target Gene of the EWSR1::FLI1 Fusion Oncoprotein, Regulates the Expression of the Focal Adhesion Protein TENSIN3
Authors: Ebegboni Vernon Justice , Jones Tamara L. , Brownmiller Tayvia , Zhao Patrick X. , Pehrsson Erica C. , Sundara Rajan Soumya , Caplen Natasha J.
Published: July 2024

Analysis of ETS1 binding
Supplementary Methods from Low- and High-Grade Glioma-Associated Vascular Cells Differentially Regulate Tumor Growth
Authors: Muthukrishnan Sree Deepthi , Qi Haocheng , Wang David , Elahi Lubayna , Pham Amy , Alvarado Alvaro G. , Li Tie , Gao Fuying , Kawaguchi Riki , Lai Albert , Kornblum Harley I.
Published: July 2024

Supplementary methods
Supplementary Figure S2 from Low- and High-Grade Glioma-Associated Vascular Cells Differentially Regulate Tumor Growth
Authors: Muthukrishnan Sree Deepthi , Qi Haocheng , Wang David , Elahi Lubayna , Pham Amy , Alvarado Alvaro G. , Li Tie , Gao Fuying , Kawaguchi Riki , Lai Albert , Kornblum Harley I.
Published: July 2024

Supplementary Figure S2. Molecular and functional differences between LG- and HG-GVC
Supplementary Data: Video 1 from ETS1, a Target Gene of the EWSR1::FLI1 Fusion Oncoprotein, Regulates the Expression of the Focal Adhesion Protein TENSIN3
Authors: Ebegboni Vernon Justice , Jones Tamara L. , Brownmiller Tayvia , Zhao Patrick X. , Pehrsson Erica C. , Sundara Rajan Soumya , Caplen Natasha J.
Published: July 2024

SK-N-MC-dCas9_VP64
Data from Hepatitis B Virus–Mediated m6A Demethylation Increases Hepatocellular Carcinoma Stemness and Immune Escape
Authors: Meng Yuting , Shu Zheyue , Wang Xueyao , Hong Liang , Wang Baohua , Jiang Jingjing , He Kangxin , Cao Qingyi , Shi Fan , Wang Hai , Gong Lan , Diao Hongyan
Published: July 2024

Abstract
Hepatitis B viral (HBV) persistent infection plays a significant role in hepatocellular carcinoma (HCC) tumorigenesis. Many studies have revealed the pivotal roles of N6-methyladenosine (m6A) in multiple cancers, while the regulatory mechanism in stemness maintenance of HBV persistent infection-related HCC remains elusive. Here, we demonstrated that the level of m6A modification was downregulated by HBV in HBV-positive HCC, through enhanced stability of ALKBH5 mRNA. More specifically, we also identified that ALKBH5 mRNA was functionally required for the stemness maintenance and self-renewal in the HBV-positive HCC, but dispensable in HBV-negative HCC. Mechanistically, ALKBH5 demethylated the m6A modification in the 3′ untranslated region of the oncogenic gene SNAI2 to prevent the recognition of YTHDF2 therewith stabilize SNAI2 transcripts, contributing to cancer stem cell traits in HBV-positive HCC. Moreover, the expression of SNAI2 reversed the suppression of stemness properties by knocking down ALKBH5. In addition, ALKBH5/SNAI2 axis accelerates tumor immune evasion through activated ligand of immune checkpoint CD155. Our study unveiled that the ALKBH5 induces m6A demethylation of the SNAI2 as a key regulator in HBV-related HCC, and identifies the function of ALKBH5/SNAI2/YTHDF2 axis in promoting the stem-like cells phenotype and immune escape during HBV infection.
Implications:
HBV promotes HCC stemness maintenance through elevate m6A modification of SNAI2 in an ALKBH5-YTHDF2–dependent manner and increases the expression of the ligand of immune checkpoint CD155.
NAP1L1 Promotes Endometrial Cancer Progression via EP300-Mediated DDX5 Promoter Acetylation
Authors: Zhu Xiangfei , Li Yishan , Shao Zhiying , Lu Xiaoyuan , Chen Youguo
Published: July 2024

Endometrial cancer is one of the predominant tumors of the female reproductive system. In this current study, we investigated the functions and related mechanisms of nucleosome assembly protein 1 like 1 (NAP1L1)/ DEAD-box helicase 5 (DDX5) in endometrial cancer. This retrospective study analyzed the medical records of patients with endometrial cancer, collected tissue samples for NAP1L1 and DDX5 staining, and conducted survival analysis using the Kaplan–Meier method. To evaluate the impact of NAP1L1 and/or DDX5 on cellular processes in endometrial cancer cells, several techniques were employed. These included Cell Counting Kit-8 assay, wound healing assay, Transwell assay, as well as overexpression or knockdown of target gene expression. Additionally, chromatin immunoprecipitation, dual luciferase reporter gene, and coimmunoprecipitation (Co-IP) assay were utilized to confirm the interaction between NAP1L1, E1A-binding protein p300 (EP300), and DDX5. Furthermore, qRT-PCR, Western blot, and Co-IP assay were performed to analyze the modulation of NAP1L1/DDX5 in Wnt/β-catenin. NAP1L1 and DDX5 expression were upregulated in endometrial cancer tissues, and correlated with poor prognosis. NAP1L1/DDX5 promoted endometrial cancer cell proliferation, migration, and invasion. NAP1L1 promotes acetylation and transcription by recruiting EP300 to the DDX5 promoter. DDX5 could activate Wnt/β-catenin signal by binding to β-catenin. In animal models, knockdown of NAP1L1 inhibits endometrial cancer tumor growth and lung metastasis. To sum up, our study demonstrated that NAP1L1 promoted the malignant phenotypes of endometrial cancer cells via recruiting EP300 to promote DDX5 acetylation, thus activating the Wnt/β-catenin signaling pathway.

Implications: Our research findings indicate that targeting the NAP1L1/EP300/DX5 axis might be a new potential treatment option for endometrial cancer.
Enhancing Colorectal Cancer Screening with Droplet Digital PCR Analysis of Fusobacterium nucleatum in Fecal Immunochemical Test Samples
Authors: Datorre José G. , dos Reis Mariana B. , de Carvalho Ana C. , Porto Jun , Rodrigues Gabriela H. , Lima Adhara B. , Reis Monise T. , Hirai Welinton , Hashimoto Claudio L. , Guimarães Denise P. , Reis Rui M.
Published: July 2024

Fecal immunochemical test (FIT) followed by colonoscopy in positive cases is commonly used for population-based colorectal cancer screening. However, specificity of FIT for colorectal cancer is not ideal and has poor performance for advanced adenoma detection. Fecal Fusobacterium nucleatum (Fn) detection has been proposed as a potential noninvasive biomarker for colorectal cancer and advanced adenoma detection. We aimed to evaluate the diagnostic performance of Fn detection using droplet digital PCR (ddPCR) in FIT samples from individuals enrolled in a colorectal cancer screening program with colorectal adenoma or cancer. We evaluated Fn presence in DNA isolated from FIT leftover material of 300 participants in a colorectal cancer screening program using ddPCR. The Fn DNA amount was classified as Fn-low/negative and Fn-high, and the association with patients’ clinicopathological features and accuracy measurements was calculated. Fn-high levels were more prevalent in FIT-positive (47.2%, n = 34 of 72) than FIT-negative samples (28.9%, n = 66 of 228; P < 0.04). Among FIT-positive samples, high Fn levels were significantly more frequent in patients with cancer (CA, n = 8) when compared to normal (NT, n = 16; P = 0.02), non-advanced adenomas (NAA, n = 36; P = 0.01), and advanced adenomas (AA, n = 12; P = 0.01). Performance analysis of Fn in FIT-positive samples for colorectal cancer detection yielded an AUC of 0.8203 [confidence interval (CI), 0.6464–0.9942], with high sensitivity (100%) and specificity of 50%. Concluding, we showed the feasibility of detecting Fn in FIT leftovers using the ultrasensitive ddPCR technique. Furthermore, we highlighted the potential use of Fn levels in fecal samples to ameliorate colorectal cancer detection.

Prevention Relevance: Fusobacterium nucleatum detection by droplet digital PCR could prioritize the selection of fecal immunochemical test–positive individuals who might benefit the most from the colonoscopy procedure.
Supplementary Table S3 from Small Extracellular Vesicle piR-hsa-30937 Derived from Pancreatic Neuroendocrine Neoplasms Upregulates CD276 in Macrophages to Promote Immune Evasion
Authors: Zhong Yuan , Tian Ye , Wang Yan , Bai Jian’an , Long Qin , Yan Lijun , Gong Zhihui , Gao Wei , Tang Qiyun
Published: July 2024

Antibodies used in assays
Supplementary Table S2 from Small Extracellular Vesicle piR-hsa-30937 Derived from Pancreatic Neuroendocrine Neoplasms Upregulates CD276 in Macrophages to Promote Immune Evasion
Authors: Zhong Yuan , Tian Ye , Wang Yan , Bai Jian’an , Long Qin , Yan Lijun , Gong Zhihui , Gao Wei , Tang Qiyun
Published: July 2024

Constructed sequences for plasmids
Supplementary Figure 2 from Melanoma Clonal Heterogeneity Leads to Secondary Resistance after Adoptive Cell Therapy with Tumor-Infiltrating Lymphocytes
Authors: König David , Sandholzer Michael T. , Uzun Sarp , Zingg Andreas , Ritschard Reto , Thut Helen , Glatz Katharina , Kappos Elisabeth A. , Schaefer Dirk J. , Kettelhack Christoph , Passweg Jakob R. , Holbro Andreas , Baur Katharina , Medinger Michael , Buser Andreas , Lardinois Didier , Jeker Lukas T. , Khanna Nina , Stenner Frank , Kasenda Benjamin , Homicsko Krisztian , Matter Matthias , Rodrigues Mantuano Natalia , Zippelius Alfred , Läubli Heinz
Published: July 2024

Supplementary Figure 2. Regressing soft tissue metastasis (Biopsy 3 weeks after TIL transfer). Vital tumor areas (SOX10) are densely infiltrated by CD8 positive tumor infiltrating lymphocytes as shown by immunohistochemistry.
Supplementary Table S2 from The CD33xCD123xCD70 Multispecific CD3-Engaging DARPin MP0533 Induces Selective T Cell–Mediated Killing of AML Leukemic Stem Cells
Authors: Bianchi Matteo , Reichen Christian , Croset Amelie , Fischer Stefanie , Eggenschwiler Aline , Grübler Yvonne , Marpakwar Rajlakshmi , Looser Thamar , Spitzli Patricia , Herzog Christel , Villemagne Denis , Schiegg Dieter , Abduli Liridon , Iss Chloé , Neculcea Alexandra , Franchini Marco , Lekishvili Tamara , Ragusa Simone , Zitt Christof , Kaufmann Yvonne , Auge Alienor , Hänggi Martin , Ali Waleed , Frasconi Teresa M. , Wullschleger Stephan , Schlegel Iris , Matzner Mirela , Lüthi Ursina , Schlereth Bernd , Dawson Keith M. , Kirkin Vladimir , Ochsenbein Adrian F. , Grimm Sebastian , Reschke Nina , Riether Carsten , Steiner Daniel , Leupin Nicolas , Goubier Anne
Published: July 2024

Supplementary Table S2
Supplementary Figure S11 from The CD33xCD123xCD70 Multispecific CD3-Engaging DARPin MP0533 Induces Selective T Cell–Mediated Killing of AML Leukemic Stem Cells
Authors: Bianchi Matteo , Reichen Christian , Croset Amelie , Fischer Stefanie , Eggenschwiler Aline , Grübler Yvonne , Marpakwar Rajlakshmi , Looser Thamar , Spitzli Patricia , Herzog Christel , Villemagne Denis , Schiegg Dieter , Abduli Liridon , Iss Chloé , Neculcea Alexandra , Franchini Marco , Lekishvili Tamara , Ragusa Simone , Zitt Christof , Kaufmann Yvonne , Auge Alienor , Hänggi Martin , Ali Waleed , Frasconi Teresa M. , Wullschleger Stephan , Schlegel Iris , Matzner Mirela , Lüthi Ursina , Schlereth Bernd , Dawson Keith M. , Kirkin Vladimir , Ochsenbein Adrian F. , Grimm Sebastian , Reschke Nina , Riether Carsten , Steiner Daniel , Leupin Nicolas , Goubier Anne
Published: July 2024

Supplementary Fig. S11. MP0533 efficacy in hPBMC mouse model xenografted subcutaneously with RPMI8226 cells.
Supplementary Figure 3 from SIRT3 Negatively Regulates TFH-Cell Differentiation in Cancer
Authors: Hou Yueru , Cao Yejin , He Ying , Dong Lin , Zhao Longhao , Dong Yingjie , Niu Ruiying , Bi Yujing , Liu Guangwei
Published: July 2024

Fig.S3. Gating strategies of TFH cells and mouse genotyping.
Supplementary Figure 16 from SIRT3 Negatively Regulates TFH-Cell Differentiation in Cancer
Authors: Hou Yueru , Cao Yejin , He Ying , Dong Lin , Zhao Longhao , Dong Yingjie , Niu Ruiying , Bi Yujing , Liu Guangwei
Published: July 2024

Fig. S16. SIRT3 deficiency upregulates the expressions of HIF1α
Figure S4 from IL3-Driven T Cell–Basophil Crosstalk Enhances Antitumor Immunity
Authors: Wei Jian , Mayberry Colleen L. , Lv Xiaoting , Hu Fangyan , Khan Taushif , Logan Natalie A. , Wilson John J. , Sears John D. , Chaussabel Damien , Chang Chih-Hao
Published: July 2024

Co-culture with bulk splenocytes potentiates antitumor activity of CD8+ T cells
Figure S3 from IL3-Driven T Cell–Basophil Crosstalk Enhances Antitumor Immunity
Authors: Wei Jian , Mayberry Colleen L. , Lv Xiaoting , Hu Fangyan , Khan Taushif , Logan Natalie A. , Wilson John J. , Sears John D. , Chaussabel Damien , Chang Chih-Hao
Published: July 2024

IL3 does not directly affect metabolism or effector function of CD8+ T cells
Supplementary Fig. S15 from Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity
Authors: Pan Haifeng , Yu Siyuan , Zhuang Haoyun , Yang Han , Jiang Jinlu , Yang Haihui , Ren Shuling , Luo Guoxing , Yu Xuan , Chen Shuping , Lin Yanhua , Sheng Roufang , Zhang Shiyin , Yuan Quan , Huang Chenghao , Zhang Tianying , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao
Published: July 2024

Supplementary Fig. S15. Analysis of the boost effect of vaccines.
Data from The CD33xCD123xCD70 Multispecific CD3-Engaging DARPin MP0533 Induces Selective T Cell–Mediated Killing of AML Leukemic Stem Cells
Authors: Bianchi Matteo , Reichen Christian , Croset Amelie , Fischer Stefanie , Eggenschwiler Aline , Grübler Yvonne , Marpakwar Rajlakshmi , Looser Thamar , Spitzli Patricia , Herzog Christel , Villemagne Denis , Schiegg Dieter , Abduli Liridon , Iss Chloé , Neculcea Alexandra , Franchini Marco , Lekishvili Tamara , Ragusa Simone , Zitt Christof , Kaufmann Yvonne , Auge Alienor , Hänggi Martin , Ali Waleed , Frasconi Teresa M. , Wullschleger Stephan , Schlegel Iris , Matzner Mirela , Lüthi Ursina , Schlereth Bernd , Dawson Keith M. , Kirkin Vladimir , Ochsenbein Adrian F. , Grimm Sebastian , Reschke Nina , Riether Carsten , Steiner Daniel , Leupin Nicolas , Goubier Anne
Published: July 2024

Abstract
The prognosis of patients with acute myeloid leukemia (AML) is limited, especially for elderly or unfit patients not eligible for hematopoietic stem cell (HSC) transplantation. The disease is driven by leukemic stem cells (LSCs), which are characterized by clonal heterogeneity and resistance to conventional therapy. These cells are therefore believed to be a major cause of progression and relapse. We designed MP0533, a multispecific CD3-engaging designed ankyrin repeat protein (DARPin) that can simultaneously bind to three antigens on AML cells (CD33, CD123, and CD70), aiming to enable avidity-driven T cell–mediated killing of AML cells coexpressing at least two of the antigens. In vitro, MP0533 induced selective T cell–mediated killing of AML cell lines, as well as patient-derived AML blasts and LSCs, expressing two or more target antigens, while sparing healthy HSCs, blood, and endothelial cells. The higher selectivity also resulted in markedly lower levels of cytokine release in normal human blood compared to single antigen–targeting T-cell engagers. In xenograft AML mice models, MP0533 induced tumor-localized T-cell activation and cytokine release, leading to complete eradication of the tumors while having no systemic adverse effects. These studies show that the multispecific-targeting strategy used with MP0533 holds promise for improved selectivity toward LSCs and efficacy against clonal heterogeneity, potentially bringing a new therapeutic option to this group of patients with a high unmet need. MP0533 is currently being evaluated in a dose-escalation phase 1 study in patients with relapsed or refractory AML (NCT05673057).
Supplementary Figure 2 from Claudin-4 Modulates Autophagy via SLC1A5/LAT1 as a Mechanism to Regulate Micronuclei
Authors: Villagomez Fabian R. , Lang Julie , Rosario Fredrick J. , Nunez-Avellaneda Daniel , Webb Patricia , Neville Margaret , Woodruff Elizabeth R. , Bitler Benjamin G.
Published: July 2024

Gating strategy for autophagic flux
Cartesian modernity: an open path?
Authors: Beserra Abel dos Santos
Published: July 2024

This article revisits a discussion presented by Marilena Chaui, in Um anacronismo interessante, to reflect on the Cartesian heritage and its appropriation, particularly by German idealism, exemplified by Kant and Hegel. Our hypothesis is that there is an equivocal reading of Descartes which establishes him as the precursor of the concept of subject as subjectivity, which would then emerge as a condition of possibility for the practice of philosophy or for its interpretation. This reading key allows us to engage in the discussion of what Alquié, in Descartes et l’ontologie négative and in A filosofia de Descartes, proposes about the status of Cartesian ideas in contemporary thought. Therefore, we conclude that it is necessary to continually renew the invitation to enter the conceptual and argumentative universe of philosophy. Furthermore, we argue the hypothesis that modernity is not a singular project but rather an ongoing one.
For the history of the Opúsculos by José Leite de Vasconcelos
Authors: Carvalheiro Vânia
Published: July 2024

The Opúsculos by José Leite de Vasconcelos contains the reprint of many of the articles published during his lifetime. It is known that the invitation for its publication was made by Joaquim de Carvalho, the Imprensa da Universidade de Coimbra’s director at the time, but little else was known apart from the information contained in the prologues of the various volumes written by the author. Recent investigations on the author’s assets at the National Archeology Museum allowed us to provide new information regarding the creation of the work from a global perspective. It is precisely this information that is presented in this article, so that knowledge can be added to a work whose history remained somewhat unknown.
Do condicionamento à expressão: investigando as raízes da criação estética na aprendizagem por associação
Authors: Nascimento Natali L.
Published: July 2024

Este ensayo explora la intersección entre el aprendizaje por asociación y el proceso estético de creación. Comenzando con una breve discusión sobre el asociacionismo, se presentan las raíces filosóficas que fundamentan la comprensión de la mente humana, desde las ideas de Immanuel Kant, David Hume y John Locke hasta las contribuciones de Gustav Theodor Fechner y otros exponentes de la psicología y neurociencia contemporáneas. Se considera cómo se exploran los principios de la Gestalt y del Conexionismo en relación con la preferencia estética. Así, se discuten los fundamentos del aprendizaje por asociación, y se destaca cómo sus procesos son moldeados por la experiencia individual y las influencias culturales que pueden, además, afectar el pensamiento crítico colectivo, recorriendo diversas aproximaciones al conocimiento. Se concluye que tales preferencias estéticas son resultado de una interacción compleja entre procesos cognitivos, experiencias individuales e influencias culturales, moldeadas tanto por la razón como por la emoción a lo largo del tiempo.
Pluteus canaliculatus (Pluteaceae: Agaricomycetes), a new species of Pluteus section Celluloderma from Turkey
Authors: KAYGUSUZ OĞUZHAN , JUSTO ALFREDO , PIEPENBRING MEIKE
Published: July 2024

During a survey of the macrofungal diversity in the Sweetgum Forest Nature Protection Area (Burdur Province), southwestern Turkey, we discovered a new species of Pluteus. The species is described and illustrated here as Pluteus canaliculatus, based on its morphological characteristics and molecular phylogenetic analyses. The new species was found on wood in a Liquidambar orientalis forest and is characterized by a light brown-yellow to reddish brown canaliculate pileus, ochre brown lamellar edges, a shiny, silvery cream stipe, predominantly ellipsoid basidiospores, mostly narrowly fusiform to fusiform pleurocystidia, often with a moniliform to flexuose neck, and an euhymeniderm or a transitional (hymeniderm and epithelium) type of pileipellis. Phylogenetic analyses of nuclear ribosomal internal transcribed spacer ITS1-5.8S-ITS2 (ITS) region sequences reveal that Pluteus canaliculatus belongs to Pluteus sect. Celluloderma, forming a distinct lineage in the /thomsonii clade. A description and a comparison with morphologically similar and closely related species are provided, together with colour photographs and line drawings of the species.
Modulation of U.S. Tornado Activity by Year-Round North American Weather Regimes
Authors: Tippett Michael K. , Malloy Kelsey , Lee Simon H.
Published: July 2024

Here, we examine the relation between U.S. tornado activity and a new year-round classification of North American weather regimes. The regime classification is based on 500-hPa geopotential height anomalies and classifies each day as Pacific Trough, Pacific Ridge, Alaskan Ridge, Greenland High, or No regime. During the period 1979–2022, we find statistically significant relations between average tornado report numbers and weather regimes in all months except June–August. Tornado activity is enhanced on Pacific Ridge days during late winter and spring, reduced on Pacific Trough days in spring, and reduced on Alaskan Ridge and Greenland High days during fall and early winter. During active regimes, the probability of many tornadoes occurring also increases, and there is greater variability in the number of tornadoes reported each day. A reanalysis-based tornado index reproduces the regional features of the modulation of tornado activity by the weather regimes and attributes them to changes in storm relative helicity, convective available potential energy, and convective precipitation. The phase of El Niño–Southern Oscillation (ENSO) also plays a role. In winter and spring, Pacific Ridge days occur more often and average more reports per day during cool ENSO conditions. During warm ENSO conditions, Pacific Trough days occur more often and are associated with widespread reduced tornado activity.

Significance Statement

Daily weather patterns over North America can be classified into five categories. The purpose of this study was to examine whether the number of U.S. tornado reports on a given day depends on the weather category of that day. We found robust relations between the average number of tornado reports and the weather pattern category in all months except June–August, with some weather patterns associated with increased tornado numbers and others with decreased tornado numbers. The El Niño–Southern Oscillation (ENSO) phenomenon plays a role, with weather patterns that are favorable for tornadoes being more frequent and having more tornadoes per day during cool ENSO conditions.
LncRNA TRPM2-AS promotes endometrial carcinoma progression and angiogenesis via targeting miR-497-5p/SPP1 axis
Authors: Ma Hanbo , Weng Fengyun , Tong Xiaowen , Li Huaifang , Yao Yinan , Yuan Jiangjing
Published: July 2024

Background

Anti-angiogenic therapy has become one of the effective treatment methods for tumors. Long noncoding RNAs (lncRNAs) are emerging as important regulators of tumorigenesis and angiogenesis in EC. However, the underlying mechanisms of lncRNA TRPM2-AS in EC are still not clear.

Methods

We screened the differently expressed lncRNAs that were highly associated with poor prognosis and angiogenesis of EC by bioinformatics analysis, and constructed a ceRNA network based on the prognostic lncRNAs. The subcellular localization of TRPM2-AS was determined by fluorescence in situ hybridization (FISH) and nuclear cytoplasmic fractionation assay. CCK-8, EdU, transwell, western blot, qRT-PCR and endothelial tube formation assay were used to evaluate the effects of TRPM2-AS on the proliferation, invasion, migration of EC cells and angiogenesis. The targeted microRNA (miRNA) of TRPM2-AS was predicted by bioinformatic methods. The interaction between TRPM2-AS and miR497-5p, miR497-5p and SPP1 were analyzed by RNA immunoprecipitation and dual-luciferase reporter assay. A subcutaneous tumor model was used to explore TRPM2-AS’s function in vivo. CIBERSORT was used to analyze the correlation between TRPM2-AS and immune cell immersion in EC.

Results

We found that the expression of TRPM2-AS and SPP1 was aberrantly upregulated, while miR-497-5p expression was significantly downregulated in EC tissues and cells. TRPM2-AS was closely correlated with the angiogenesis and poor prognosis in EC patients. Mechanistically, TRPM2-AS could sponge miR-497-5p to release SPP1, thus promoting the proliferation, invasion and migration of EC cells and angiogenesis of HUVECs. Knockdown of TRPM2-AS in xenograft mouse model inhibited tumor proliferation and angiogenesis in vivo. In addition, TRPM2-AS plays a vital role in regulating the tumor immune microenvironment of EC, overexpression of TRPM2-AS in EC cells stimulated the polarization of M2 macrophages and angiogenesis through secreting SPP1 enriched exosomes.

Conclusion

The depletion of TRPM2-AS inhibits the oncogenicity of EC by targeting the miR-497-5p/SPP1 axis. This study offers a better understanding of TRPM2-AS’s role in regulating angiogenesis and provides a novel target for EC treatment.
Extracellular vesicle miRNAs for predicting the efficacy of late-line treatment with anlotinib in patients with lung adenocarcinoma
Authors: Huang Aimi , Zhang Fuchuang , Zhang Jiyang , Xu Xiaoya , Li Zhikuan , Chen Sheng , Nian Baoning , Zhang Dadong , Han Baohui , Gu Aiqin , Wang Weimin
Published: July 2024

Background

Anlotinib is a targeted therapy indicated for some malignancies, including advanced non-small cell lung cancer (NSCLC). However, noninvasive biomarkers for identifying patients who will benefit from this disease remain lacking.

Methods

Here, we investigated the potential of small extracellular vesicle (sEV) microRNAs (miRNAs) as predictive biomarkers for anlotinib efficacy. A total of 20 advanced NSCLC patients were enrolled. Patients were classified as having stable disease (SD) or progressive disease (PD) after the initial efficacy assessment.

Results

Seven differentially expressed miRNAs (DEMs) were identified. Among them, miR-941 was significantly upregulated in the PD group, while the others were downregulated. Furthermore, these six downregulated miRNAs (miR-30a-3p, miR-150-5p, miR-122-5p, miR-10b-5p, miR-92a-3p, and miR-150-3p) were more pronounced in nonsmoking patients.

Conclusions

It was found that sEV miRNAs have the potential to predict the benefit of anlotinib.
Application of remote sensing to understand the role of Galician feral horses in the biomass reduction of a shrub-grassland-dominated landscape
Authors: Janeiro-Otero Andrea , Álvarez Xana , Dormann Carsten F.
Published: July 2024

Galician forests in northwestern Spain are subject to frequent wildfires with high environmental and economic costs. In addition, due to the consequences of climate change, these fires are becoming more virulent, occurring throughout the year, and taking place in populated areas, in some cases involving the loss of human life. Therefore, forest fire prevention is even more relevant than mitigating its consequences. Given the costs involved in forestry work, alternative measures to reduce fuel load and create vegetation gaps are needed. One involves grazing by an endemic species of feral horses (Equus ferus atlanticus) that feed on thicket-forming gorse (Ulex europaeus). In a 100-ha forest fenced study area stocked with 11 horses, four 50 m2 enclosed plots prevented the access of these wild animals to the vegetation, with the aim of manipulating their impact on the reduction of forest biomass. The measurement of biomass volumes is an important method that can describe the assessment of wildfire risks, unfortunately, high-resolution data collection at the regional scale is very time-consuming. The best result can be using drones (unmanned aerial vehicles - UAVs) as a method of collecting remotely sensed data at low cost. From September 2018 to November 2020, we collected information about aboveground biomass from these four enclosed plots and their surrounding areas available for horses to forage, via UAV. These data, together with environmental variables from the study site, were used as input for a fire model to assess the differences in the surface rate of spread (SROS) among grazed and ungrazed areas. Our results indicated a consistent but small reduction in the SROS between 0.55 and 3.10 m/min in the ungrazed enclosured plots in comparison to their grazed surrounding areas (which have an SROS between 15 and 25 m/min). The research showed that radar remote sensing (UAV) can be used to map forest aboveground biomass, and emphasized the importance and role of feral horses in Galicia as a prevention tool against wildfires in gorse-dominated landscapes.
Regulatory gene network for coffee-like color morph of TYRP1 mutant of oujiang color common carp
Authors: Mandal Roland Nathan , Ke Jing , Kanika Nusrat Hasan , Wang Fuyan , Wang Jun , Wang Chenghui
Published: July 2024

Background

Neither a TYRP1-mediated highly conserved genetic network underlying skin color towards optimum defense nor the pathological tendency of its mutation is well understood. The Oujiang Color Common Carp (Cyprinus carpio var. color) as a model organism, offering valuable insights into genetics, coloration, aquaculture practices, and environmental health. Here, we performed a comparative skin transcriptome analysis on TYRP1 mutant and wild fishes by applying a conservative categorical approach considering different color phenotypes.

Results

Our results reveal that an unusual color phenotype may be sensitized with TYRP1 mutation as a result of upregulating several genes related to an anti-inflammatory autoimmune system in response to the COMT-mediated catecholamine neurotransmitters in the skin. Particularly, catecholamines-derived red/brown, red with blue colored membrane attack complex, and brown/grey colored reduced eumelanin are expected to be aggregated in the regenerated cells.

Conclusions

It is, thus, concluded that the regenerated cells with catecholamines, membrane attack complex, and eumelanin altogether may contribute to the formation of the unusual (coffee-like) color phenotype in TYRP1 mutant.
Effect of pericapsular nerve group block and suprainguinal fascia iliaca block on postoperative analgesia and stress response in elderly patients undergoing hip arthroplasty: a prospective randomized controlled double-blind trial
Authors: Cui Xiaozhen , Cheng Zhi , Zhang Tianyu , Xu Hai , Luan Hengfei , Feng Jiying , Zhang Xiaobao , Zhu Pin
Published: July 2024

Background

As a novel regional analgesic technique, ultrasound-guided pericapsular nerve group (PENG) block has some potential advantages, and we designed a randomized clinical trial (RCT) to investigate whether the ultrasound-guided PENG block combined with general anesthesia can better reduce stress response, maintain intraoperative hemodynamic stability, and reduce postoperative analgesia in elderly hip arthroplasty compared with ultrasound-guided suprainguinal fascia iliaca block (SIFIB) combined with general anesthesia.

Methods

Seventy-four subjects were enrolled over an 8-month period (20 April 2023 to 31 December 2023). All patients were divided into the test group (group P) and the control group (group S) using the envelope as the randomization method. The test group was treated with preoperative ultrasound-guided PENG block analgesia combined with general anesthesia and the control group was treated with preoperative ultrasound-guided SIFIB analgesia combined with general anesthesia. The primary outcome selected was the patient Visual Analogue Scale (VAS) score at 12 h postoperatively.

Results

After generalized estimating equations (GEE) analysis, there was a statistically significant difference in the main effect of postoperative VAS score in group P compared with group S (P = 0.009), the time effect of VAS score in each group was significantly different (P < 0.001), and there was no statistically significant difference in the group-time interaction effect (P = 0.069). There was no statistically significant difference in the main effect of intraoperative mean arterial pressure (MAP) change (P = 0.911), there were statistically significant differences in the time effect of MAP in each group (P < 0.001), and there were statistically significant differences in the interaction effect (P < 0.001).

Conclusions

In summary, we can conclude that in elderly patients undergoing hip fracture surgery, postoperative analgesia is more pronounced, intraoperative hemodynamic parameters are more stable, and intraoperative stress is less induced in patients receiving SIFIB than in patients receiving PENG block.
Ambulance professionals' experiences of teamwork in the context of a team training programme – a qualitative study
Authors: Myhr Kjetil , Ballangrud Randi , Aase Karina , Vifladt Anne
Published: July 2024

Background

Teamwork in the context of ambulance services exhibits unique characteristics, as this environment involves a small core team that must adapt to a dynamic team structure that involves health care professionals and emergency services. It is essential to acquire a deeper understanding of how ambulance teams operate. Therefore, this study aimed to explore the experiences of ambulance professionals with teamwork and how they were influenced by the implementation of a team training programme.

Methods

A qualitative descriptive study was conducted involving ambulance professionals who took part in focus group interviews carried out both before and after the implementation of a team training program across seven ambulance stations within a Norwegian hospital trust. The data were analysed using reflexive thematic analysis based on a deductive-inductive approach.

Results

Our analysis revealed 15 subthemes that characterised ambulance professionals’ experiences with teamwork and a team training programme, which were organised according to the five main themes of team structure, communication, leadership, situation monitoring, and mutual support. Ambulance professionals’ experiences ranged from the significance of team composition and interpersonal and professional relationships to their preferences regarding different communication styles and the necessity of team leaders within the ambulance service. The team training programme raised awareness of teamwork, while the adoption of teamwork tools was influenced by both individual and contextual factors. The Introduction/Identity, Situation, Background, Assessment and Recommendation (ISBAR) communication tool was identified as the most beneficial aspect of the programme due to its ease of use, which led to improvements in the structure and quality of consultations and information handover.

Conclusions

This study documented the diverse characteristics and preferences associated with teamwork among ambulance professionals, emphasising the particular importance of proficient partnerships in this context. Participation in a team training programme was perceived as a valuable reminder of the significance of teamwork, thus providing a foundation for the enhancement of communication skills.

Trial registration

ClinicalTrials.gov—ID: NCT05244928.
Agreement between Clinical Frailty Scale-scores based on information from patient interviews and Clinical Frailty Scale-scores based on information from medical records - a cross sectional study
Authors: Jackwert Kim , Holmér Michael , Hallongren Matilda , Asmar Todel , Wretenberg Per , Andersson Åsa G
Published: July 2024

Introduction

Frailty is an age-related condition with increased risk for adverse health outcomes. Assessing frailty according to the Clinical Frailty Scale (CFS) based on data from medical records is useful for previously unassessed patients, but the validity of such scores in exclusively geriatric populations and in patients with dementia is relatively unknown.

Methods

Patients admitted for the first time to one of two geriatric wards at Örebro University hospital between January 1st – December 31st, 2021, were included in this study if they had been appointed a CFS-score by anamnestic interview (CFSI) at admission. CFS scores based on medical records (CFSR) were appointed by a single medical student, who was blinded to the CFSI score. Score-agreement was evaluated with quadratic weighted Cohen’s kappa (κ).

Results

In total, 145 patients between the age of 55–101 were included in the study. The CFSR and CFSI scores agreed perfectly in 102 cases (0.7, 95% CI 0.65–0.77). There was no significant difference regarding age, sex, comorbidity, or number of patients diagnosed with dementia between the patients with complete agreement and the patients whose scores did not agree. Agreement between the scores was substantial, κ = 0.66, 95% CI 0.53–0.80.

Conclusions

CFS scores based on information from medical records can be generated with substantial agreement to CFS scores based on in-person anamnestic interviews. A dementia diagnosis does not influence the agreement between the scores. Therefore, these scores are a useful tool for assessing frailty in geriatric patients who previously lack a frailty assessment, both in clinical practice and future research. The results support previous findings, but larger studies are warranted.
Association between HIV and acquisition of rifamycin resistance with first-line TB treatment: a systematic review and meta-analysis
Authors: Zinyakatira Nesbert , Ford Nathan , Cox Helen
Published: July 2024

Background

Multi-drug or rifamycin-resistant tuberculosis (MDR/RR-TB) is an important public health concern, including in settings with high HIV prevalence. TB drug resistance can be directly transmitted or arise through resistance acquisition during first-line TB treatment. Limited evidence suggests that people living with HIV (PLHIV) might have an increased risk of acquired rifamycin-resistance (ARR).

Methods

To assess HIV as a risk factor for ARR during first-line TB treatment, a systematic review and meta-analysis was conducted. ARR was defined as rifamycin-susceptibility at treatment start with rifamycin-resistance diagnosed during or at the end of treatment, or at recurrence. PubMed/MEDLINE, CINAHL, Cochrane Library, and Google Scholar databases were searched from inception to 23 May 2024 for articles in English; conference abstracts were also searched from 2004 to 2021. The Mantel-Haenszel random-effects model was used to estimate the pooled odds ratio of any association between HIV and ARR among individuals receiving first-line TB treatment.

Results

Ten studies that included data collected between 1990 and 2014 were identified: five from the United States, two from South Africa and one each from Uganda, India and Moldova. A total of 97,564 individuals were included across all studies, with 13,359 (13.7%) PLHIV. Overall, 312 (0.32%) acquired rifamycin-resistance, among whom 115 (36.9%) were PLHIV. The weighted odds of ARR were 4.57 (95% CI, 2.01–10.42) times higher among PLHIV compared to HIV-negative individuals receiving first-line TB treatment.

Conclusion

The available data, suggest that PLHIV have an increased ARR risk during first-line TB treatment. Further research is needed to clarify specific risk factors, including advanced HIV disease and TB disease severity. Given the introduction of shorter, 4-month rifamycin-based regimens, there is an urgent need for additional data on ARR, particularly for PLHIV.

Systematic review registration

PROSPERO CRD42022327337.
Test-treat-track-test-treat (5T) approach for Schistosoma haematobium elimination on Pemba Island, Tanzania
Authors: Trippler Lydia , Taylor Lyndsay , Ali Mohammed Nassor , Najim Sarah Omar , Khamis Khamis Seif , Hattendorf Jan , Juma Saleh , Ame Shaali Makame , Kabole Fatma , Ali Said Mohammed , Knopp Stefanie
Published: July 2024

Background

After decades of praziquantel mass drug administration (MDA), several countries approach schistosomiasis elimination. Continuing MDA in largely uninfected populations no longer seems justified. Alternative interventions to maintain the gains or accelerate interruption of transmission are needed. We report results, strengths, and shortcomings of novel test-treat-track-test-treat (5T) interventions in low Schistosoma haematobium prevalence areas on Pemba, Tanzania.

Methods

School- and household-based surveys were conducted in 2021 and 2022 to monitor the S. haematobium and microhematuria prevalence and assess the impact of interventions. In 2021, 5T interventions were implemented in 15 low-prevalence areas and included: (i) testing schoolchildren in primary and Islamic schools for microhematuria as a proxy for S. haematobium, (ii) treating positive children, (iii) tracking them to their households and to water bodies they frequented, (iv) testing individuals at households and water bodies, and (v) treating positive individuals. Additionally, test-and-treat interventions were implemented in the 22 health facilities of the study area.

Results

The S. haematobium prevalence in the school-based survey in 15 low-prevalence implementation units was 0.5% (7/1560) in 2021 and 0.4% (6/1645) in 2022. In the household-based survey, 0.5% (14/2975) and 0.7% (19/2920) of participants were infected with S. haematobium in 2021 and 2022, respectively. The microhematuria prevalence, excluding trace results, in the school-based survey was 1.4% (21/1560) in 2021 and 1.5% (24/1645) in 2022. In the household-based survey, it was 3.3% (98/2975) in 2021 and 5.4% (159/2920) in 2022. During the 5T interventions, the microhaematuria prevalence was 3.8% (140/3700) and 5.8% (34/594) in children in primary and Islamic schools, respectively, 17.1% (44/258) in household members, and 16.7% (10/60) in people at water bodies. In health facilities, 19.8% (70/354) of patients tested microhematuria-positive.

Conclusions

The targeted 5T interventions maintained the very low S. haematobium prevalence and proved straightforward and feasible to identify and treat many of the few S. haematobium-infected individuals. Future research will show whether 5T interventions can maintain gains in the longer-term and expedite elimination.

Trial registration

ISRCTN, ISCRCTN91431493. Registered 11 February 2020, https://www.isrctn.com/ISRCTN91431493.