bluuebunny/crossref_metadata_2025 huggingface dataset:
Fiber Tensioning Systems in a Robotized Winding Procedure for Composite Materials Building Processes
Authors: Sbanca Mădălin Petru , Boboc Răzvan Gabriel , Mogan Gheorghe Leonte
Published: January 2023

The winding process of carbon fiber is not a new concept, but until now only a few studies have been made in carbon fiber tensioning and winding on complex shapes. The main purpose of the article is to analyze the possibility to conduct the carbon fiber winding using two industrial robots connected as master slave, one to realize the winding and the other to hold and rotate the mandrel, using an own design of an automatic fiber tensioning tool. To have control of the system a force transducer was implemented. The measured force was used to make trajectory adjustments for the robots, having a predefined trajectory. The results obtained from the experimental tests showed that the winding process with two robots and a tensioning system can realize fiber windings with variable tension in molds with complex configurations (3D) of the winding paths. In this way, compact structures of the constantly tensioned fiber bundle can be obtained, according to product requirements.
ACPA-Net: Atrous Channel Pyramid Attention Network for Segmentation of Leakage in Rail Tunnel Linings
Authors: Geng Peng , Tan Ziye , Luo Jun , Wang Tongming , Li Feng , Bei Jianghui
Published: January 2023

The automatic segmentation of leakage in rail tunnel linings is a useful and challenging task. Unlike other scenarios, the complex environment inside the tunnels makes it difficult to obtain accurate results for the segmentation of leakages. Some deep learning-based methods have been used to automatically segment tunnel leakage, but these methods ignore the interdependencies between feature channels, which are very important for extracting robust leakage feature representations. In this work, we propose an atrous channel pyramid attention network (ACPA-Net) for rail tunnel lining leakage segmentation. In ACPA-Net, the proposed atrous channel pyramid attention (ACPA) module is added into a U-shaped segmentation network. The ACPA module can effectively strengthen the representation ability of ACPA-Net by explicitly modeling the dependencies between feature channels. In addition, a deep supervision strategy that helps ACPA-Net improve its discrimination ability has also been introduced into ACPA-Net. A rail tunnel leakage image dataset consisting of 1370 images with manual annotation maps is built to verify the effectiveness of ACPA-Net. The final experiment shows that ACPA-Net achieves state-of-the-art performance on the Crack500 dataset and our rail tunnel leakage image dataset, and our method has the least number of parameters of all the methods.
Secure Multi-Party Computation of Graphs’ Intersection and Union under the Malicious Model
Authors: Liu Xin , Tu Xiao-Fen , Luo Dan , Xu Gang , Xiong Neal , Chen Xiu-Bo
Published: January 2023

In recent years, with the development of information security, secure multi-party computation has gradually become a research hotspot in the field of privacy protection. The intersection and union computation of graphs is an important branch of secure computing geometry. At present, the intersection and union of graphs are almost designed under the semi-honest model, and few solutions are proposed under the malicious model. However, the solution under the malicious model is more secure and has important theoretical and practical significance. In this paper, the possible malicious behaviors of computing the intersection and union of graphs are analyzed. Using the Lifted-ElGamal threshold cryptosystem and zero-knowledge proof method, the secure multi-party computation algorithm of graphs’ intersection and union under the malicious model is designed. The real/ideal model paradigm is used to prove the security of the algorithm, the efficiency of the algorithm is analyzed in detail, and the feasibility is verified through experiment.
Doctoral Supervision: A Best Practice Review
Authors: Polkinghorne Martyn , Taylor Julia , Knight Fiona , Stewart Natalie
Published: January 2023

A “doctoral student” is the term for a student undertaking the highest level of university degree (a doctorate). “Supervisor” is the term for the academic, or academics, who act as their guide. Unlike taught classroom-based degree courses, doctoral degrees in the UK are normally only, or mainly, focused upon a single intensive research study into a specific topic. Such degree courses facilitate the development of students into highly specialist autonomous researchers capable of independent thought. Typically, a blend of support is provided to each doctoral student which consists of an elective development program of research methods learning opportunities alongside dedicated supervisor support from one or more academic members of staff called “supervisors”. It is the expectation that each supervisor will act as a guide and mentor for the doctoral student, thereby enabling them to successfully complete their program of research. This entry relates primarily to the UK model of supervising a doctoral student. Doctoral programs in other countries may differ.
Consciousness: A Strategy for Behavioral Decisions
Authors: Grinde Bjørn
Published: January 2023

Most multicellular animals have a nervous system that is based on the following three components: (1) sensory cells gather information and send it to processing units; (2) the processing units use the information to decide what action to take; and (3) effector neurons activate the appropriate muscles. Due to the importance of making the right decisions, evolution made profound advances in processing units. I review present knowledge regarding the evolution of neurological tools for making decisions, here referred to as strategies or algorithms. Consciousness can be understood as a particularly sophisticated strategy. It may have evolved to allow for the use of feelings as a ‘common currency’ to evaluate behavioral options. The advanced cognitive capacity of species such as humans further improved the usefulness of consciousness, yet in biological terms, it does not seem to be an optimal, fitness-enhancing strategy. A model for the gradual evolution of consciousness is presented. There is a somewhat arbitrary cutoff as to which animals have consciousness, but based on current information, it seems reasonable to restrict the term to amniotes.
Prediction of Native Seed Habitat Distribution According to SSP Scenario and Seed Transfer Zones: A Focus on Acer pictum subsp. mono and Quercus acuta
Authors: Kim Chaeyoung , Kim Wheemoon , Song Wonkyong , Cho Jaepil , Choi Jaeyong
Published: January 2023

Acer pictum and Quercus acuta are native species recommended for restoration. To restore ecosystem functions and maintain natural ecosystems, it is suggested to deploy well-adapted and locally adapted plant material, and this notion is gaining interest. Studying how species change in response to climate change is an important part of forest restoration planning. Our method uses climate data to define the habitat range of species and to identify regions with relatively similar climates through Seed Transfer Zones (STZs). Potential habitat suitability changes of A. pictum and Q. acuta were identified under various environmental scenarios using seven climatic factors and five topographical factors. The MaxEnt algorithm was used to predict potential habitat suitability in current and future (1980–2100) climate change scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0, SSP5-8.5). STZs are maps of areas with comparable climates and have been used to determine the climates of potentially habitable areas. This helps to minimize the maladaptation of seed movement within the same area. As a result, A. pictum growth increased along the southern coastal area and drainage was the paramount factor influencing A. pictum distribution. By checking the climate of regions with high habitability in STZs (Winter Minimum Temperature (WMT) 15–20 °F, Annual Heat: Moisture (AHM) 16–19 °C/m zone, WMT 20–25 °F, AHM 16–19 °C/m located in the zone), Q. acuta was shown to move northward with climate change centering on the southern coastal area. Additionally, Annual Precipitation (Bio12) was the most significant factor influencing Q. acuta distribution. In confirming the climate of areas with high habitability in STZs, we verified that habitat density was high in the WMT 10–15 °F, AHM 19–21 °C/m zone and WMT 20–25 °F, AHM 19–21 °C/m zone. This study establishes that the potential distributions of A. pictum and Q. acuta are affected by climate change. It supplies evidence for ecological restoration and sustainable development, and can formulate future conservation and management plans for economically valuable species.
Exploring the Role of Weather and Forest Management on Nutrient Export in Boreal Forested Catchments Using Spatially Distributed Model
Authors: Salmivaara Aura , Leinonen Antti , Palviainen Marjo , Korhonen Natalia , Launiainen Samuli , Tuomenvirta Heikki , Ukonmaanaho Liisa , Finér Leena , Laurén Annamari (Ari)
Published: January 2023

Weather-driven hydrological variability and forest management influence the nutrient export from terrestrial to aquatic systems. We quantified the effect and range of variation in total nitrogen and phosphorus export in Vehka-Kuonanjärvi catchment located in southeastern Finland. A distributed model NutSpaFHy was used with varying weather scenarios (compiled from observed extreme years of dry, wet and wet & mild) and forest management scenarios (including no additional management and intensive clear-cutting of all mature stands in the existing forest structure). Nutrient exports by scenario combinations were compared to modeled baseline export in observed weather. The results showed that the increase in nutrient export by wet & mild weather (over 55%) exceeded the increase caused by the clear-cutting scenario (23 %). Dry weather decreased the exports to tenth of the baseline, which was per hectare 2.22 kg for N, 0.08 kg for P). The results suggest that in future maintaining a good ecological status in aquatic systems can be challenging if extreme wet years with mild winters occur more frequently. Certain catchment characteristics, e.g., deciduous tree percentage, open area percentage and site fertility, influence the export increase induced by the extreme weather. Hotspot analysis enabled identifying areas with currently high nutrient export and areas with high increase induced by the extreme weather. This helps targeting water protection efficiently.
Pathways and Characteristics of Lead Uptake and Transportation in Rhus chinensis Mill
Authors: He Wenxiang , Wang Shufeng , Wang Yangdong , Lu Mengzhu , Shi Xiang
Published: January 2023

Rhus chinensis Mill is a potential plant for phytoremediation of Pb and is able to uptake a copious amount of Pb. However, little is known about the pathways and properties of Pb uptake in this plant. Here, controlled experiments were used to assess Pb uptake and translocation in R. chinensis. The whole time-kinetics of Pb uptake were divided into two stages: rapid uptake and slower accumulation, and the two processes were fitted with a linear model. The concentration-dependent kinetics of Pb uptake were characterized by a modified Michaelis–Menten equation. The Km and Vmax value of Pb influx in roots were 19.44 and 14.83, respectively. Transpiration inhibitors had no significant effect on the Pb concentration of root and shoot. Low temperatures (4 °C) and metabolic inhibitors (carbonyl cyanide m-chlorophenylhydrazone and 2,4-dinitrophenol) significantly reduced the Pb concentration in the roots and leaves of R. chinensis plants. Furthermore, the addition of calcium ion channel inhibitors and protein synthesis inhibitors significantly reduced the Pb concentration in the roots of R. chinensis plants. These results show that both active and passive processes of Pb uptake and translocation exist in the roots of R. chinensis plants. In addition, Pb uptake by the roots of R. chinensis plants was related to calcium ion channels.
Ruminal Degradation of Taurine and Its Effects on Rumen Fermentation In Vitro
Authors: Zhang Shuo , Liang Qinyue , Li Mengmeng , Zhao Guangyong
Published: January 2023

Taurine accounts for approximately 0.1% of an animal’s body. It cannot be used for protein synthesis but plays a wide range of important roles in the animal body. Taurine does not exist in plants, while mammals can only synthesize 30–40% of the taurine they need. Supplementing taurine to beef cattle may be necessary to improve their nutrient utilization and health status. However, no data are available regarding the metabolism of taurine in the rumen. Two in vitro trials were conducted to investigate the ruminal degradability of taurine and its effects on rumen fermentation. In Trial 1, Tilley and Terry’s in vitro rumen fermentation technique was used for incubation. As treatments, two levels of taurine, i.e., 0 and 10 mg, were added into plastic tubes containing 0.4000 g of feed mixture with a calibrated volume of 50 mL. Three adult cattle fitted with rumen cannulas were used as the donors for rumen fluid. The incubation was carried out at 39 °C for 48 h. The results showed that the taurine degradability increased with incubation time (p < 0.001) while its 2 h-degradability reached 99%. Taurine decreased the 48 h-dry matter degradability (DMD) (p = 0.008) and increased the 24 h- and 48 h-pH (p = 0.005; p = 0.018), respectively. In Trial 2, the Hohenheim gas test was used for incubation. Four levels of taurine, i.e., 0, 5, 10 and 20 mg, were added into glass syringes containing 0.2000 g feed mixture with a calibrated volume of 100 mL as treatments. The rumen fluid donors were the same as in Trial 1. The incubation was carried out at 39 °C for 48 h. The results showed that taurine increased the 48 h-pH (p < 0.001) linearly, decreased the cumulative gas production (p < 0.001) and the total volatile fatty acids (VFA) concentration (p = 0.014), and quadratically affected the ammonia–nitrogen (p < 0.001) and microbial crude protein (MCP) concentrations (p < 0.001). It was concluded that taurine was highly degradable in rumen fermentation. Taurine inhibits ruminal fermentation by decreasing DMD, VFA and gas production while improving MCP synthesis on a dose-dependent basis.
Edwardsiella ictaluri Almost Completely Occupies the Gut Microbiota of Fish Suffering from Enteric Septicemia of Catfish (Esc)
Authors: Yang Jicheng , Lin Yaoyao , Wei Zhaohui , Wu Zhenbing , Zhang Qianqian , Hao Jingwen , Wang Shuyi , Li Aihua
Published: January 2023

To reveal the changes in the gut microbiota of yellow catfish after being infected by Edwardsiella ictaluri, 16S rRNA gene high-throughput sequencing technology was used to analyze the microbial composition and diversity of the healthy and diseased yellow catfish. The gut microbial richness and diversity of the diseased fish were significantly lower than those of healthy fish. The composition and predicted function of yellow catfish gut microbiota were drastically altered after infection by E. ictaluri. Fusobacteriota, Proteobacteria, and Firmicutes were the predominant bacterial phyla in the gut of the healthy fish, while Proteobacteria was the dominant phylum in the gut of the diseased fish. At the genus level, the gut of healthy fish was dominated by Cetobacterium, Plesiomonas, and Romboutsia, while the gut of diseased fish was overwhelmed by the pathogenic E. ictaluri (99.22 ± 0.85%), and Cetobacterium, Plesiomonas, and Romboutsia disappeared. This is the most characteristic feature of the intestinal microbiota composition of yellow catfish edwardsiellosis. The same sequence of E. ictaluri was detected in the intestine of the healthy fish and the liver and intestine of the diseased fish. The anaerobic and Gram-positive bacteria were significantly decreased, and the digestive system, immune system, and metabolic functions of the gut microbiota were significantly reduced in the diseased fish gut. This may be part of the pathogenesis of fish edwardsiellosis.
Hematological Response of Juvenile Cobia to Three Anesthetics
Authors: Sorensen Karl , Craig Steven R. , Cnaani Avner , McLean Ewen
Published: January 2023

Optimal concentrations of three anesthetics for use with juvenile cobia (Rachycentron canadum) were determined using time-to-recovery and hematological datasets. Buffered MS-222, clove oil and 2-phenoxyethanol (2-PE) were examined. Juvenile cobia were exposed to three concentrations of each anesthetic at 24 °C. Based on time to recovery, optimal doses for MS-222 was determined as 120-mg L−1, that for 2-PE, 0.4-mL L−1 and, for clove oil, 5-mL L−1. The hematological response of cobia to anesthesia included quantification of whole blood pH, pCO2, pO2, and Ca2+, K+, Na+, Cl−, hematocrit and glucose. Irrespective of anesthetic employed, cobia expressed metabolic acidosis, with changes in blood pH (p < 0.001) being matched by increases (p < 0.001) in pCO2. Anesthesia tended to increase blood Na+, hematocrit, pO2, Ca2+, and K+ although differential responses between anesthetics were recorded, suggesting different modes of action. A combination of recovery and hematological data indicated that when anesthesia is necessary, MS-222 represents the sedative of choice.
Evaluating the Utility of Five Gene Fragments for Genetic Diversity Analyses of Mytella strigata Populations
Authors: Zuo Chenxia , Zhang Tingting , Zhang Chenchen , Zhao Daotan , Zhu Yi , Ma Xiaojie , Wang Haiyan , Ma Peizhen , Zhang Zhen
Published: January 2023

Mytella strigata (Hanley, 1843) is an invasive mussel species that has rapidly spread in China in recent years. Here, we tested the utility of three mitochondrial gene fragments, COI, 12S, and 16S, and two nuclear gene fragments, D1 28S and 18S-ITS1, for characterizing the levels of genetic diversity among and within populations using 191 M. strigata specimens collected in China to aid ongoing efforts to identify the origin of the invasion as well as molecular genetic studies. M. strigata exhibited two sex-associated haplogroups according to the COI and 12S sequences. The ratio of female-lineage to male-lineage COI and 12S sequences was 149:22 and 72:7, and the genetic distances between haplogroups were 6.56 and 9.17, respectively. Only one haplotype was detected among the 18S-ITS1 sequences (413 bp), and three haplotypes were detected among the D1 28S sequences (296 bp). The haplotype diversity of both the female-lineage COI and 12S sequences was greater than 0.5, and the nucleotide diversity of the 12S, 16S, D1 28S, and 18S-ITS1 sequences was less than 0.005 in all six populations in China. Our findings indicated that COI is the most useful gene fragment for genetic diversity studies of M. strigata populations; D1 28S and 18S-ITS1 sequences would be useful for species identification because of their low intraspecific diversity. Our genetic analysis of the COI sequences revealed Colombia as the most likely origin of M. strigata in China and showed that the invasive populations in China have recently experienced or are currently experiencing a population bottleneck.
The Effects of Gamma-Aminobuytric Acid (GABA) Enrichment on Nutritional, Physical, Shelf-Life, and Sensorial Properties of Dark Chocolate
Authors: Koh Wee Yin , Lim Xiao Xian , Teoh Eva Sheue Wen , Kobun Rovina , Rasti Babak
Published: January 2023

Hypertension is the leading cause of cardiovascular disease and premature death worldwide. Gamma-aminobutyric acid (GABA) has potential in regulating hypertension. Cocoa beans are rich in GABA, but GABA is being destroyed during roasting of cocoa beans and chocolate production. This study aimed to develop GABA-enriched dark chocolate by partially replacing sugar syrup with pure GABA powder at concentrations of 0.05 (F1), 0.10 (F2), and 0.15% (F3). The chocolate samples were incorporated with GABA after the heating and melting process of cocoa butter to maintain the viability and functionality of the GABA in the final product. The effects of GABA enrichment on the quality of chocolate in terms of nutritional, physical, shelf-life, and sensorial properties were studied. The inclusion of 0.15% GABA significantly increased the GABA content and angiotensin-converting-enzyme (ACE) inhibitory effect of chocolate. The nutritional compositions of the control and GABA-enriched chocolates were almost similar. The addition of GABA significantly increased the hardness but did not affect the apparent viscosity and melting properties of chocolate. Accelerated shelf-life test results showed that all the chocolates stored at 20 and 30 °C were microbiologically safe for consumption for at least 21 days. Among the GABA-enriched chocolates, panellists preferred F2 the most followed by F3 and F1, owing to the glossiness and sweetness of F2. F3 with the highest GABA content (21.09 mg/100 g) and ACE inhibitory effect (79.54%) was identified as the best GABA-enriched dark chocolate.
Feeding Lactic Acid Bacteria with Different Sugars: Effect on Exopolysaccharides (EPS) Production and Their Molecular Characteristics
Authors: Fuso Andrea , Bancalari Elena , Castellone Vincenzo , Caligiani Augusta , Gatti Monica , Bottari Benedetta
Published: January 2023

Exopolysaccharides (EPS) are complex molecules produced by some microorganisms and used in foods as texturizers and stabilizers, their properties depending on their chemical structure. In this work, three different lactic acid bacteria (LAB), were tested for their ability to produce EPS, by using five different mono- and disaccharides as their sole carbon source. The growth and acidifying ability were analysed, the EPSs were quantified by the official method AOAC 991.43, and their chemical structure was investigated. The amount of EPS varied from 0.71 g/L to 2.38 g/L, and maltose was the best sugar for EPS production by Lacticaseibacillus paracasei 2333. Lacticaseibacillus rhamnosus 1019 produced the highest amount when fed with lactose, whereas the EPS amount of Lactobacillus bulgaricus 1932 was not significantly different depending on the sugar type. The EPS chains consisted of fructose, galactose, glucose, mannose, ribose, glucosamine, galactosamine, and in some cases rhamnose in different proportions, depending on the strain and carbon source. The molecular weight of EPS ranged from <10 KDa to >500 KDa and was again highly dependent on the strain and the sugar used, suggesting the possibility of growing different strains under different conditions to obtain EPS with different potential applications in the food system.
Internal Quality Attributes and Sensory Characteristics of ‘Ambrosia’ Apples with Different Dry Matter Content after a Two-Week and a Ten-Week Air Storage at 1 °C
Authors: Bejaei Masoumeh , Xu Hao
Published: January 2023

This research was conducted to determine the compositional and textural characteristics and sensory profile of ‘Ambrosia’ apples with different dry matter content (DMC) as estimated using a Felix-750 Produce Quality Meter (Felix Instruments Inc., Camas, WA, USA). Fruits were harvested from a commercial orchard in Cawston and an experimental field in Summerland Research and Development Centre (SuRDC) in British Columbia, Canada, when the average absorbance difference index/coefficient of fruit skin δAbsorbance (δA) dropped under 0.45 ± 0.10. DMC levels were estimated after harvest at the blush/background transition zone for fruit categorization on 300 fruits from each location. Fruits were coded with an individual number and grouped in different DMC categories. The distribution of the estimated DMC levels obtained from two locations was different. The results indicate that DMC levels were strongly and positively correlated with the soluble solids content (SSC) of the fruit (r = 0.81). Sensory evaluations also demonstrated that apples in the lowest DMC category (12.5% ± 0.5 from Cawston) were considered the least sweet apples with the least overall flavour quality by panellists compared to the apples from the other DMC categories included in the sensory evaluations from the two locations. Panellists also perceived less-than-expected “fresh apple” and “tropical” flavours but more-than-expected “no flavour” and “bland” off flavour from the lowest-DMC-category apples. The non-destructive DMC measurements show a potential to be used to sort apples for SSC, sweetness and flavour; nevertheless, they were not related to firmness or textural attributes.
The Effect of Sodium Nitroprusside Treatment on Storage Ability of Fresh-Cut Potato
Authors: Dai Yukexin , Xie Hong , Zhao Xiaoyan , Zheng Yanyan
Published: January 2023

Quality deterioration is a major problem restricting the fresh-cut potato industry. The present study investigated the effect of sodium nitroprusside (SNP) treatment on the quality of fresh-cut potatoes during short-term storage. The treatment was carried out immediately either before or after cutting, using an SNP concentration of 200 μmol/L. The results showed that SNP treatment inhibited the accumulation of malondialdehyde (MDA) and total soluble solids (TSSs). SNP treatment also decreased the firmness, chewing properties, and ascorbic acid (AsA) content in potatoes, maintaining high levels of total phenols (TPs), total flavonoids (TFs), nitric oxide (NO), and superoxide dismutase (SOD). Furthermore, SNP treatment restrained the rise of phenylalanine ammonia-lyase (PAL), peroxidase (POD), and polyphenol oxidase (PPO), as well as the electrolyte leakage (EL) rate. After SNP treatment, the nitrite content in the potatoes was within security scope. Comparing potatoes treated before and after cutting, the best result was noted in the potatoes soaked in SNP before cutting, which displayed the smallest losses in firmness (11.24%), chewing properties (34.30%), and AsA (40.35%), and maximum increases in TPs (32.84%), TFs (2.83−time), NO (76.11%), and SOD activity (93.15%). Moreover, this group presented the minimum MDA content, EL rate, and TSS values and the lowest PAL, POD, and PPO activities. These results indicated that 200 μmol/L SNP applied for 20 min, particularly before cutting, is an efficient alternative technology that can be used in the fresh-cut potato industry.
Identification of a Novel Walnut Iron Chelating Peptide with Potential High Antioxidant Activity and Analysis of Its Possible Binding Sites
Authors: Fan Chaozhong , Wang Xintong , Song Xiwang , Sun Ronghao , Liu Rui , Sui Wenjie , Jin Yan , Wu Tao , Zhang Min
Published: January 2023

Peptide iron chelate is widely regarded as one of the best iron supplements for relieving iron deficiency. In this study, a new type of walnut peptide iron (WP-Fe) chelate was prepared using low molecular weight walnut peptides (WP) as raw materials. Under the conditions of this study, the chelation rate and iron content of the WP-Fe chelate were 71.87 ± 1.60% and 113.11 ± 2.52 mg/g, respectively. Fourier transform infrared spectroscopy (FTIR), zeta potential, amino acid composition, and other structural analysis showed that WP-Fe is formed by the combination of carboxyl, amino and carbonyl with Fe2+. The WP-Fe chelate exhibits a honeycomb-like bulk structure different from that of WP. In addition, we predicted and established the binding model of ferrous ion and WP by molecular docking technology. After chelation, the free radical scavenging ability of the WP-Fe chelate was significantly higher than that of the WP. Overall, the WP-Fe chelate has high iron-binding capacity and antioxidant activity. We believe that peptides from different sources also have better iron binding capacity, and peptide iron chelates are expected to become a promising source of iron supplement and antioxidant activities.
Response Surface Methodology Optimization for Analytical Microwave-Assisted Extraction of Resveratrol from Functional Marmalade and Cookies
Authors: Setyaningsih Widiastuti , Guamán-Balcázar María del Cisne , Oktaviani Nurul Mutmainah Diah , Palma Miguel
Published: January 2023

A novel analytical method based on microwave-assisted extraction has been successfully optimized and validated to determine resveratrol from functional marmalade and cookies. The optimization was performed using a Box–Behnken design with three factors: solvent composition (60−100% and 10−70% methanol in water for marmalade and cookies, respectively), microwave power (250−750 W), and solvent-to-solid ratio (20:5−60:5). The main and quadratic effects of solvent composition significantly contributed to the recovery values (p < 0.005) for both kinds of samples. Additionally, the solvent-to-solid ratio and the quadratic effect of microwave power also influenced the resveratrol recovery from functional marmalade. Hence, the optimum condition for resveratrol extraction from marmalade (80% methanol, 500 W, solvent-to-solid ratio 40:5) and cookies (80% methanol, 250 W, solvent-to-solid ratio 20:5) was proposed. The extraction kinetics (5−30 min) was then studied to clarify the complete recovery of resveratrol from the food matrices resulting in 15 min as the optimum extraction time. The developed method showed a satisfactory precision indicated by the coefficient of variation (CV) lower than 5.70% for both repeatability and intermediate precision. To check the matrix effects, the developed MAE procedures were applied to a number of commercial marmalade and cookies. The high-fat and fiber cookies resulted in overestimated values due to the interfering ingredients. As a final point, the methods successfully measured the stability of naturally present resveratrol in grape-derived products while preparing functional marmalade and cookies.
Inhibition of Iron Release from Donkey Spleen Ferritin through Malt-Derived Protein Z–Ferulic Acid Interactions
Authors: Sun Mingyang , Liu Hanhan , Xu Chen , Jiang Zhenghui , Lv Chenyan
Published: January 2023

Protein–small molecule interactions naturally occur in foodstuffs, which could improve the properties of protein and small molecules. Meanwhile, they might affect the bioavailability and nutritional value of proteins. Ferritin, as an iron-storage protein, has been a focus of research. However, the complexity of foodstuffs enables the interaction between ferritin and food components, especially polyphenols, which can induce iron release from ferritin. Thus, the application of ferritin in food is limited. Inspired by the natural-occurring, strong protein–polyphenol interactions in beer, to inhibit the iron release of ferritin, the malt-derived protein Z (PZ) was chosen to interact with ferulic acid (FA), an abundant reductant in malt, beer, and other foodstuffs. The analysis of the interaction between PZ and FA was carried out using fluorescence spectroscopy, the results of which suggest that one PZ molecule can bind with 22.11 ± 2.13 of FA, and the binding constant is (4.99 ± 2.13) × 105 M−1. In a molecular dynamics (MD) simulation, FA was found to be embedded in the internal hydrophobic pocket of PZ, where it formed hydrogen bonds with Val-389 and Tyr-234. As expected, compared to iron release induced by FA, the iron release from donkey spleen ferritin (DSF) induced by FA decreased by 86.20% in the presence of PZ. Meanwhile, based on the PZ–FA interaction, adding PZ in beer reduced iron release from DSF by 40.5% when DSF:PZ was 1:40 (molar ratio). This work will provide a novel method of inhibiting iron release from ferritin.
A Qualitative Assessment of the Deployment of Zero-Emission Heavy-Duty Trucks in Logistics—Deriving Recommendations for Action from a Socio-Technical Approach on the Regional Level
Authors: Müller Christoph
Published: January 2023

Although different European and national policies have set targets for a shift from fossil fuels to zero-emission heavy-duty trucks in the transport sector, their share is still rather low and mainly on a project level. The process of changing human perception in freight transport goes beyond the often discussed technical or economical aspects and demands research activities and perspectives from different stakeholders. Therefore, this paper delivers a stakeholder analysis together with a qualitative comprehensive analysis from different perspectives. It reveals the influence and importance as well as the general attitude of identified stakeholders concerning zero-emission heavy-duty trucks in logistics on a regional level in order to identify changes and barriers and to derive recommendations for action. The carried out socio-technical approach uses and adapts existing and approved scientific approaches with the general aim of creating a holistic assessment to accompany the development and implementation of zero-emission drive-systems in the transport sector.
The Impact of Organizer Market Structure on Participant Entry Behavior in a Multi-Tournament Environment
Authors: Mathews Timothy , Namoro Soiliou Daw , Boudreau James W.
Published: January 2023

A multi-tournament environment is analyzed, focusing on the impact of organizer market structure on agent entry behavior. Two high ability agents first decide which tournament to enter (with fields then filled by low ability agents). If the marginal benefit of high ability agents in an event is weakly increasing, a monopsonist organizer sets prizes so that the high ability agents enter the same event. If this marginal benefit is diminishing, a monopsonist organizer will either: always set prizes for which the high ability agents enter different events; or set prizes for which the high ability agents enter different events if and only if the difference in ability between the high ability and low ability agents is sufficiently small. Sequentially competing organizers set prizes for which both high ability agents enter the same event if and only if the marginal benefit of having two high ability agents in one event is relatively large. For competing organizers there may be either a first or second mover advantage. Finally, Social Welfare may be higher or lower with competing organizers, implying greater organizer competition does not necessarily increase Social Welfare. Parallels are noted throughout to the labor market for professional golfers both over years when the PGA TOUR was essentially a monopsonist and more recently when LIV Golf emerged as a competitor.
Alginate Hydrogels Reinforced by Dehydration under Stress—Application to a Soft Magnetic Actuator
Authors: Leon-Cecilla Alberto , Vazquez-Perez Francisco J. , Gila-Vilchez Cristina , Álvarez de Cienfuegos Luis , Lopez-Lopez Modesto T.
Published: January 2023

We investigated the effect of partial dehydration under mechanical stress in the properties of alginate hydrogels. For this aim, we characterized the mechanical properties of the hydrogels under tensile and shear stress, as well as their swelling behavior, macroscopic appearance, and microscopic structure. We found that the processes of dehydration under a mechanical stress were irreversible with fully rehydration being impossible. What is more, these processes gave rise to an enhancement of the mechanical robustness of the hydrogels beyond the effect due to the increase in polymer concentration caused by dehydration. Finally, we analyzed the applicability of these results to alginate-based magnetic hydrogel grippers that bended in response to an applied magnetic field. Remarkably, our study demonstrated that the dehydration of the magnetic hydrogels under compression facilitated their bending response.
A Novel Approach, Based on the Combined Action of Chitosan Hydrogel and Laccases, for the Removal of Dyes from Textile Industry Wastewaters
Authors: Sannino Filomena , Di Matteo Elena , Ambrosecchio Mariarosaria , Pirozzi Domenico
Published: January 2023

Dyes are considered as one the most important classes of contaminants that threaten the environment and human life. The synergy between the adsorption capacity of chitosan hydrogels and the catalytic properties of the enzyme laccase was exploited to improve the removal of contaminants from a liquid stream. The adsorption capacity of a chitosan hydrogel was tested on three different textile dyes. The effect of pH on the adsorption efficiency was dependent on the dye tested: the removal of methylene blue (MB), a cationic dye, was more effective at alkaline values of pH, whereas bromophenol blue (BPB) and Coomassie brilliant blue (BB), both anionic dyes, were more effectively removed under acid environments. The use of laccase immobilized onto chitosan has significantly improved the efficiency of dye removal, exploiting the synergy between the adsorption capacity of chitosan and the catalytic properties of the enzyme. The simultaneous processes of adsorption and enzymatic degradation improved the dye removal whatever the pH value adopted, making the removal efficiency less dependent from the pH changes. The chitosan used as a support for the immobilization of laccases showed good stability under repeated cycles, demonstrating the feasibility of the method developed for the application in wastewater remediation.
Advancement in Human Face Prediction Using DNA
Authors: Alshehhi Aamer , Almarzooqi Aliya , Alhammadi Khadija , Werghi Naoufel , Tay Guan K. , Alsafar Habiba
Published: January 2023

The rapid improvements in identifying the genetic factors contributing to facial morphology have enabled the early identification of craniofacial syndromes. Similarly, this technology can be vital in forensic cases involving human identification from biological traces or human remains, especially when reference samples are not available in the deoxyribose nucleic acid (DNA) database. This review summarizes the currently used methods for predicting human phenotypes such as age, ancestry, pigmentation, and facial features based on genetic variations. To identify the facial features affected by DNA, various two-dimensional (2D)- and three-dimensional (3D)-scanning techniques and analysis tools are reviewed. A comparison between the scanning technologies is also presented in this review. Face-landmarking techniques and face-phenotyping algorithms are discussed in chronological order. Then, the latest approaches in genetic to 3D face shape analysis are emphasized. A systematic review of the current markers that passed the threshold of a genome-wide association (GWAS) of single nucleotide polymorphism (SNP)-face traits from the GWAS Catalog is also provided using the preferred reporting items for systematic reviews and meta-analyses (PRISMA), approach. Finally, the current challenges in forensic DNA phenotyping are analyzed and discussed.
Transcriptomic Profiling Reveals an Enhancer RNA Signature for Recurrence Prediction in Colorectal Cancer
Authors: Sahu Divya , Lin Chen-Ching , Goel Ajay
Published: January 2023

Background: Colorectal cancer (CRC) is one of the most fatal malignancies worldwide, and this is in part due to high rates of tumor recurrence in these patients. Currently, TNM staging remains the gold standard for predicting prognosis and recurrence in CRC patients; however, this approach is inadequate for identifying high-risk patients with the highest likelihood of disease recurrence. Recent evidence has revealed that enhancer RNAs (eRNAs) represent a higher level of cellular regulation, and their expression is frequently dysregulated in several cancers, including CRC. However, the clinical significance of eRNAs as recurrence predictor biomarkers in CRC remains unexplored, which is the primary aim of this study. Results: We performed a systematic analysis of eRNA expression profiles in colon cancer (CC) and rectal cancer (RC) patients from the TCGA dataset. By using rigorous biomarker discovery approaches by splitting the entire dataset into a training and testing cohort, we identified a 22-eRNA panel in CC and a 19-eRNA panel in RC for predicting tumor recurrence. The Kaplan–Meier analysis showed that biomarker panels robustly stratified low and high-risk CC (p = 7.29 × 10−5) and RC (p = 6.81 × 10−3) patients with recurrence. Multivariate and LASSO Cox regression models indicated that both biomarker panels were independent predictors of recurrence and significantly superior to TNM staging in CC (HR = 11.89, p = 9.54 × 10−4) and RC (HR = 3.91, p = 3.52 × 10−2). Notably, the ROC curves demonstrated that both panels exhibited excellent recurrence prediction accuracy in CC (AUC = 0.833; 95% CI: 0.74–0.93) and RC (AUC = 0.834; 95% CI: 0.72–0.92) patients. Subsequently, a combination signature that included the eRNA panels and TNM staging achieved an even greater predictive accuracy in patients with CC (AUC = 0.85). Conclusions: Herein, we report a novel eRNA signature for predicting recurrence in patients with CRC. Further experimental validation in independent clinical cohorts, these biomarkers can potentially improve current risk stratification approaches for guiding precision oncology treatments in patients suffering from this lethal malignancy.
Analysis of CAT Gene Family and Functional Identification of OsCAT3 in Rice
Authors: Jiang Wenxiang , Ye Qing , Wu Zheng , Zhang Qiuyun , Wang Lianhong , Liu Jialin , Hu Xiafei , Guo Dandan , Wang Xiaoqing , Zhang Zelin , He Haohua , Hu Lifang
Published: January 2023

Catalase (CAT) is an important antioxidant enzyme in plants that plays a key role in plant growth and stress responses. CAT is usually encoded by a small gene family that has been cloned and functionally studied in some species, such as Arabidopsis, wheat and cucumber, but its specific roles in rice are not clear at present. In this study, we identified three CAT family genes (OsCAT1, OsCAT2 and OsCAT3) in the rice genome and performed a systematic bioinformatics analysis. RT−PCR analysis revealed that OsCAT1–OsCAT3 was primarily expressed in vegetative tissues such as roots, stems and leaves. Since OsCAT3 showed the highest expression level among the three OsCAT genes, we then focused on its related functions. OsCAT3 prokaryotic expression protein has an obvious ability to remove H2O2. The OsCAT3crispr plant was short and had a low survival rate, the leaves were small with brown lesions, and the activities of the CAT, POD and SOD enzymes were significantly reduced. A microarray analysis showed that differentially expressed genes were primarily enriched in toxin metabolism and photosynthesis. This study laid a foundation for further understanding the function of the rice OsCAT gene.
The Assassination of Lieutenant Joe Petrosino: A Contested Symbol in the Mainstream and Italian-American Press in the Early 20th Century
Authors: Cacioppo Marina
Published: January 2023

Recently, scholars have looked at the ethnic press through a social constructionist lens, examining the process through which immigrants developed a sense of identity and the role of print culture in forming “imagined communities” (Anderson). Here, I analyze the coverage of the 1909 assassination of police Lieutenant Petrosino in both mainstream and Italian-American press and popular culture. This shocking event ignited a debate over the nature and origin of the Mafia and the dangerousness of the Italian community, a debate involving discourses of racial difference, immigration restriction, and the capability of Italians to assimilate. This debate became an important arena in which Italian immigrants defined themselves and their place in America. Italians were represented predominantly in the context of criminality, so immigrant writers constructed their own counter-representations in newspapers, re-coding stereotypes and negotiating a collective Italian-American identity. In the press, Petrosino became a contested symbol: on the one hand, of the rhetoric of the inclusiveness of the American melting pot and, on the other, of the possibility of redemption from the discredit that the actions of a small minority threw on the whole community.
A Mixed-Methods Sequential Explanatory Study of the Factors That Impact Nurses’ Perspectives toward Nurse Practitioners’ Roles in Saudi Arabia
Authors: Kerari Ali , Bahari Ghareeb , Aldossery Nahed , Qadhi Omaimah , Alghamdi Alya
Published: January 2023

Nurse practitioners’ roles need to be clearly defined in Saudi Arabia. Therefore, we aimed to explore potential factors that impact nurses’ perspectives toward nurse practitioners’ roles and whether they are interested in becoming nurse practitioners. A mixed-methods study design was employed using a questionnaire and focus groups. The survey was sent to nurses working at a public hospital in the Riyadh region. Participants (N = 77) reported that having more nurse practitioners would improve quality of care and patient safety. Additionally, most participants stated that an increased supply of nurse practitioners would have a positive impact on effectiveness, equity of care, and healthcare costs. In regression analysis, participants with favorable perspectives towards nurse practitioners were significantly more likely to have interest in becoming nurse practitioners (odds ratio [95% confidence interval]:1.04 [1.01–1.07]). In the qualitative domain, three positive factors were identified: effective collaboration with other staff, better contribution to quality care and patient safety, and better contribution to evidence-based practice. Two barriers were also determined: lack of motivation to become a nurse practitioner and unclear scope of practice. Results showed that nurse practitioners can provide quality healthcare services that meet patients’ different needs. The growing role of this speciality warrants further research to show its value in daily practice.
The Structures and Activities of Health Promotion in the Italian NHS
Authors: Bosco Roberta , Messina Gabriele , Aiello Bruno , Guarducci Giovanni , Nante Nicola
Published: January 2023

Background: In Italy, the Ministry of Health is the main decision-making entity in healthcare. The local health authorities (LHAs) are responsible for health promotion (HP) activities, based on national and regional health plans. Our aim was to investigate the structured activities of HP in Italy at national, regional, and territorial levels. Methods: From February 2020 to July 2021, we searched for online information about the structures, projects, and responsibilities at the different levels mentioned above. The sources were the official sites of the Ministry of Health, the regions, and LHAs. Results: During the “prevalence period” of 2014–2021, we found 41 active facilities dedicated to HP: 7 complex operational units and 34 simple units. The other 30 facilities also had HP activities despite the absence of dedicated units. The most discussed topic seemed to be physical activity (63%), followed by addictions (53%), nutrition (48%), and prevention (33%); in the queue appeared dental hygiene and family/parenting (both at 7%). The LHA of the City of Turin and the LHA of Salerno had the most significant number of topics. Conclusions: The results showed great heterogeneity, in the Italian context, concerning HP activities. We assume that the phenomenon depends on reduced attention to the digitalization of information. The Italian Society of Health Promotion is pursuing the goal of the construction of an organic system of HP—with its own articulations, competencies, and scientific and operational goals—at different levels, thus transcending the health care system (which is often powerless in regulatory activity) and providing the one harbinger of the most promising results in terms of cost/benefit.