bluuebunny/crossref_metadata_2025 huggingface dataset:
The impact of gradient variable temperature fermentation on the quality of cigar tobacco leaves
Authors: Zhang Qianying , Huang Yang , An Hongyue , Yang Shuanghong , Lei Jinshan , Wang Yue , Li Pinhe , Zhang Hongfei , Cai Wen , Jia Yun , Pang Yongqiang , Li Dongliang
Published: December 2024

Introduction

In order to enhance the quality of cigar tobacco leaves (CTLs), a gradient variable temperature fermentation approach was employed.

Methods

The temperature gradient demonstrated a gradual increase from low temperature (35 ± 2°C) to moderate temperature (45 ± 2°C), and then to high temperature (55 ± 2°C). Each temperature gradient underwent a 10-day fermentation process, resulting in a total duration of 30 days. Changes in sensory evaluation, chemical composition, and bacterial absolute quantitative structure and function were examined throughout the process of gradient variable temperature fermentation.

Results

Compared to constant temperature fermentation, gradient variable temperature fermentation improved the sensory quality of CTLs, reduced total sugar and cembrane degradation products, and increased the amino acid contents. It resulted in significant changes in bacterial quantity and function of CTLs, but had no significant effects on the richness and diversity of bacterial communities. The results of correlation analyses showed that sensory quality had significant correlation with chemical composition, which effected by predominant microbes. The gradient variable temperature fermentation process underwent a three-phase model to characterize the alterations of CTLs. Phase I (35°C) was the microbial stage, during which there was a significant decrease in both the total number and function of microorganisms. The dominant genera shifted from Acinetobacter to Staphylococcus and Corynebacterium, and with high reducing sugar, polyphenol compound and low sensory score. Phase II (45°C) marked a chemical stage, with an enhancement in sensory evaluation. A total of 17 chemicals significantly decreased and six increased, and the decline of microbial populations persisted. The enhanced relative abundances of four microecological hubs, namely Staphylococcus, Corynebacterium, Oceanobacillus, and Bacillus, had the potential to produce protease and lipase to the production of peptides, amino acids, and organic acid, catabolizing sugars and polyphenol compounds, through carbohydrate metabolism and amino acid metabolism, resulted an increase in sensory quality of CTLs. Phase III (55°C) indicated a relative mature stage with the highest score of sensory evaluation. Eight compositions from plamochromic pigments and polyphenol compounds exhibited gradual decreases, while relative contents of carbohydrate metabolism and amino acid metabolism increased.

Conclusion

The gradient variable temperature fermentation had demonstrated a significant positive influence on the quality of CTL by providing optimal fermentation temperature for microbial growth, metabolism, and the generation of quality-related chemical compositions.
Harnessing lactic acid bacteria for nicotinamide mononucleotide biosynthesis: a review of strategies and future directions
Authors: Kong Linghui , Li Xinyu , Liu Taiyu , Yao Qingshou , Qin Jiayang
Published: December 2024

Nicotinamide mononucleotide (NMN), one of the crucial precursors of nicotinamide adenine dinucleotide, has garnered considerable interest for its pharmacological and anti-aging effects, conferring potential health and economic benefits for humans. Lactic acid bacteria (LAB) are one of the most important probiotics, which is commonly used in the dairy industry. Due to its probiotic properties, it presents an attractive platform for food-grade NMN production. LAB have also been extensively utilized to enhance the functional properties of pharmaceuticals and cosmetics, making them promising candidates for large-scale up synthesis of NMN. This review provides an in-depth analysis of various metabolic engineering strategies, including enzyme optimization, pathway rewiring, and fermentation process enhancements, to increase NMN yields in LAB. It explores both CRISPR/Cas9 and traditional methods to manipulate key biosynthetic pathways. In particular, this study discussed future research directions, emphasizing the application of synthetic biology, systems biology, and AI-driven optimization to further enhance NMN production. It provides invaluable insights into developing scalable and industrially relevant processes for NMN production to meet the growing market demand.
Characterization and bioefficacy of grapevine bacterial endophytes against Colletotrichum gloeosporioides causing anthracnose disease
Authors: Holkar Somnath K. , Bhanbhane Vrushali C. , Ghotgalkar Prabhavati S. , Markad Harshavardhan N. , Lodha Tushar D. , Saha Sujoy , Banerjee Kaushik
Published: December 2024

Introduction

Grapevine (Vitis vinifera L.), one of the economically important fruit crops cultivated worldwide, harbours diverse endophytic bacteria (EBs) responsible for managing various fungal diseases. Anthracnose (Colletotrichum gloeosporioides) (Penz.) is one of the major constraints in quality grape production and therefore its management is a major concern among the grape growers.

Materials and methods

Among the 50 EBs isolated from healthy leaf segments from the eight grapevine genotypes, biologically potential 20 EBs were purified and identified based on morphological, and biological characteristics and sequence analysis of 16S rRNA region. The antagonistic activities of EBs against Colletotrichum gloeosporioides were studied in vitro conditions.

Results

The colony morphologies of EBs are white and yellow-coloured colonies, circular to irregular in shape, and entire, and flat margins. Among the 20 purified EBs, 19 isolates were found to be Gram-positive except one i.e., MS2 isolate. The 12 isolates reduced nitrate and 14 isolates produced urease enzyme. The in vitro assay revealed that two isolates, SB4 and RF1, inhibited 56.1% and 55.6% mycelial growth of C. gloeosporioides, respectively. Further, the identity of EBs was confirmed through PCR amplification of the 16S rRNA region resulting in ~1400 bp size amplicons. The sequence analysis of representative 15 isolates revealed that 5 EB isolates viz., SB5, CS2, RG1, RF1, C1 were identified as Bacillus subtilis with >99% sequence identity, two EBs viz., SB3, and CS1 were identified as B. subtilis subsp. subtilis, two EBs viz., SB1, and CS4 were identified as B. licheniformis. The SB2 isolate was identified as Bacillus sp., whereas SB4 as Brevibacillus borstelensis, TH1 as B. velezensis, TH2 as B. tequilensis, CS3 as B. pumilus and MS1 as Micrococcus luteus were identified.

Conclusion

The phylogenetic analysis of 16S rRNA sequence revealed eight distinct clades and showed the close clustering of identified species with the reference species retrieved from NCBI GenBank. The current investigation provides the scope for further field evaluations of these endophytic microbes for managing anthracnose disease.
Copeptin as biomarker for acute ischemic stroke prognosis and revascularization treatment efficacy
Authors: Vasile Antonia Ioana , Tiu Cristina , Badiu Corin
Published: December 2024

Introduction

Pro-arginine vasopressin consists of three peptides: arginine-vasopressin, neurophysin II, and copeptin. AVP is released by the neurohypophysis in response to increased plasma osmolality, decreased blood volume and stress. Copeptin has the advantage of being stable ex vivo and easy to measure. New data show the importance of copeptin in ischemic stroke and its complications.

Methods

We present a literature review that highlights the importance of copeptin as a biological marker for stroke. We searched the Pubmed and Scopus databases for papers with the following keywords: “stroke AND copeptin.” PRISMA criteria were used.

Results

We identified 332 papers that met the criteria. We excluded analyzed reviews, systematic reviews and meta-analyses. 31 articles resulted. The number of patients included in the analyzed studies varied between 18 and 4,302. Copeptin is a marker that associated with clinical stroke severity, infarct volume, short-term and long-term functionality and mortality and adds prognostic value to the previously used scales. It may reflect the effectiveness of revascularization therapy. Copeptin is a biomarker that can help predict post-stroke complications such as: cerebral edema and hemorrhagic transformation.

Discussion

Copeptin is a novel and promising biomarker for evaluating cerebrovascular diseases. Because it is considered a non-specific biomarker, it is not yet used routinely and it cannot replace the clinical examination. However, combined with other clinical or paraclinical parameters, it can increase the accuracy of the diagnosis.
Association between 25(OH) vitamin D and schizophrenia: shared genetic correlation, pleiotropy, and causality
Authors: Rong Guo-Wei , Li Xiao-Min , Lu Hui-Min , Su Ming-Zhu , Jin Yi
Published: December 2024

Background

This study delves into the complex interplay between genetics, 25-hydroxyvitamin D (25OHD), and schizophrenia (SCZ). It leverages extensive sample data derived from Genome-Wide Association Studies (GWAS) to uncover genetic correlations.

Methods

Employing Linkage Disequilibrium Score Regression (LDSC) and S-LDSC, this study investigates genetic connections between 25OHD and SCZ. It examines Single Nucleotide Polymorphism (SNP) heritability in specific tissues and incorporates diverse immune cell datasets for genetic enrichment analysis. Local genetic correlations were analyzed using HESS software, and pleiotropy analysis identified shared genetic loci in brain tissues. Hyprcoloc analysis was used to explore shared genetic factors between 25OHD, immune cells, and SCZ, complemented by a bidirectional Mendelian Randomization (MR) to probe potential causal links.

Results

We identified a significant negative genetic correlation between 25OHD levels and SCZ. PLACO analysis revealed 35 pleiotropic loci with strong enrichment in brain regions, particularly the cerebellum, frontal cortex, and hippocampus. Eight loci (1p34.2, 2p23.3, 3p21.1, 5q31.2, 12q23.2, 14q32.33, 16p13.3, and 16q24.3) exhibited strong colocalization, highlighting potential drug targets. Gene and tissue enrichment analyses emphasized neurological and immune-related mechanisms, including hyaluronan metabolism. Bidirectional MR analysis supported a causal effect of SCZ on 25OHD levels.

Conclusion

Our study identifies NEK4 as a potential therapeutic target and highlights the involvement of hyaluronan metabolism in the genetic association between 25OHD and SCZ. These findings provide valuable insights into shared genetic pathways, immune-related connections, and causal interactions in the context of SCZ.
Liuweizhiji Gegen-Sangshen beverage protects against alcoholic liver disease in mice through the gut microbiota mediated SCFAs/GPR43/GLP-1 pathway
Authors: Tang Mingyun , Zhao Long , Huang Fuchun , Wang Tiangang , Wu Xu , Chen Shanshan , Fu Juan , Jiang Chaoli , Wei Shulin , Zeng Xuseng , Zhang Xiaoling , Zhou Xin , Wei Mei , Li Zhi , Xiao Guohui
Published: December 2024

Introduction

Alcoholic liver disease (ALD) is a pathological state of the liver caused by longterm alcohol consumption. Recent studies have shown that the modulation of the gut microbiota and its metabolic products, specifically the short-chain fatty acids (SCFAs), exert a critical role in the evolution and progression of ALD. The Liuweizhiji Gegen-Sangshen beverage (LGS), as a functional beverage in China, is derived from a traditional Chinese herbal formula and has been clinically applied for ALD treatment, demonstrating significant efficacy. However, the underlying mechanisms of LGS for alleviating ALD involving gut microbiota regulation remain unknown.

Methods

In this study, an ALD murine model based on the National Institute on Alcohol Abuse and Alcoholism (NIAAA) method was established.

Results

The results showed that oral LGS treatment dose-dependently alleviated alcoholinduced liver injury and inflammation in mice through decreasing levels of ALT, AST and proinflammatory cytokines (TNF-α, IL-6, IL-1β). LGS significantly improved liver steatosis, enhanced activities of alcohol metabolizing enzymes (ALDH and ADH), and reduced the CYP2E1 activity. Notably, regarding most detected indices, the effect of LGS (particularly at medium and high dose) was comparable to the positive drug MTDX. Moreover, LGS had a favorable effect on maintaining intestinal barrier function through reducing epithelial injury and increasing expression of occludin. 16S rRNA sequencing results showed that LGS remarkably modulated gut microbiota structure in ALD mice via recovering alcohol-induced microbial changes and specifically mediating enrichment of several bacterial genera (Alloprevotella, Monoglobus, Erysipelatoclostridium Parasutterella, Harryflintia and unclassified_c_Clostridia). Further study revealed that LGS increased production of SCFAs of hexanoic acid in cecum, promoted alcohol-mediated reduction of GRP43 expression in ileum, and increased serum GLP-1 level.

Discussion

Overall, LGS exerts a remarkable protective effect on ALD mice through the gut microbiota mediated specific hexanoic acid production and GPR43/GLP-1 pathway.
Coexistence of a novel SV2B-ALK, EML4-ALK double-fusion in a lung poorly differentiated adenocarcinoma patient and response to alectinib: a case report and literature review
Authors: Chen Huang , Zhang Menglan , Bai Liyan , Niu Yun , Wang Xiaowei , Jiang Ruiying , Wang Ye , Feng Qianqian , Wang Bei , Dai Tingli , Yuan Mingming , Chen Rongrong , Qi Yujuan , Zhong Dingrong
Published: December 2024

Background

Anaplastic lymphoma kinase (ALK) rearrangement, the most common oncogenic rearrangement in lung adenocarcinoma, occurs in approximately 5% of non-small cell lung cancer (NSCLC) patients. EML4 gene is the most common partner of ALK rearrangement, and distinct EML4-ALK fusions differ in their responsiveness to ALK tyrosine kinase inhibitors. However, the concurrence of two ALK rearrangements in one patient and whose response to ALK-TKIs have rarely been reported so far.

Case presentation

A 47-year-old Chinese male was diagnosed with stage IV lung adenocarcinoma with multiple intracranial metastases and adrenal metastasis. After progression of two lines of chemotherapy combined with local radiotherapy regimens, his tumor tissue sample was sent to perform the DNA-based next-generation sequencing of 116 genes. Surprisingly, EML4-ALK (E13:A20) fusion and a novel SV2B-ALK (S6:A20) fusion were concurrently identified, which was confirmed using immunohistochemistry and fluorescence in-situ hybridization. Given the superior efficacy of alectinib, the patient received alectinib in the third-line setting with the progression-free survival over 14 months up to now. Moreover, through comprehensive review of previous literatures, a total of 22 patients with multiple ALK fusions and their response to ALK-TKIs were summarized.

Conclusion

This is the first report of a NSCLC patient with a novel SV2B-ALK, EML4-ALK double-fusion benefiting from alectinib. Alectinib may be an effective therapeutic option for both primary and metastatic lesions including brain metastases in the late-line setting in NSCLC patients with double-ALK fusion.
Nutraceuticals silybin B, resveratrol, and epigallocatechin-3 gallate-bind to cardiac muscle troponin to restore the loss of lusitropy caused by cardiomyopathy mutations in vitro, in vivo, and in silico
Authors: Yang Zeyu , Sheehan Alice M. , Messer Andrew E. , Tsui Sharmane , Sparrow Alexander , Redwood Charles , Kren Vladimir , Gould Ian R. , Marston Steven B.
Published: December 2024

Introduction

Adrenergic activation of protein kinase A (PKA) in cardiac muscle targets the sarcolemma, sarcoplasmic reticulum, and contractile apparatus to increase contractile force and heart rate. In the thin filaments of the contractile apparatus, cardiac troponin I (cTnI) Ser22 and Ser23 in the cardiac-specific N-terminal peptide (NcTnI: residues 1 to 32) are the targets for PKA phosphorylation. Phosphorylation causes a 2-3 fold decrease of affinity of cTn for Ca2+ associated with a higher rate of Ca2+ dissociation from cTnC leading to a faster relaxation rate of the cardiac muscle (lusitropy). Cardiomyopathy-linked mutations primarily affect Ca2+ regulation or the PKA-dependent modulatory system, such that Ca2+-sensitivity becomes independent of phosphorylation level (uncoupling) and this could be sufficient to induce cardiomyopathy. A drug that could restore the phosphorylation-dependent modulation of Ca2+-sensitivity could have potential for treatment of these pathologies. We have found that a number of small molecules, including silybin B, resveratrol and EGCG, can restore coupling in single filament assays.

Methods

We did molecular dynamics simulations (5x1500ns for each condition) of the unphosphorylated and phosphorylated cardiac troponin core with the G159D DCM mutation in the presence of the 5 ligands and analysed the effects on several dynamic parameters. We also studied the effect of the ligands on the contractility of cardiac muscle myocytes with ACTC E99K and TNNT2 R92Q mutations in response to dobutamine.

Results

Silybin B, EGCG and resveratrol restored the phosphorylation-induced change in molecular dynamics to wild-type values, whilst silybin A, an inactive isomer of silybin B, and Epicatechin gallate, an EGCG analogue that does not recouple, did not. We analysed the atomic-level changes induced by ligand binding to explain recoupling. Mutations ACTC E99K and TNNT2 R92Q blunt the increased relaxation speed response to β1 adrenergic stimulation of cardiac myocytes and we found that resveratrol, EGCG and silybin B could restore the β1 adrenergic response, whereas silybin A did not.

Discussion

The uncoupling phenomenon caused by cardiomyopathy-related mutations and the ability of small molecules to restore coupling in vitro and lusitropy in myocytes is observed at the cellular, molecular and atomistic levels therefore, restoring lusitropy is a suitable target for treatment. Further research on compounds that restore lusitropy is thus indicated as treatments for genetic cardiomyopathies. Further molecular dynamics simulations could define the specific properties needed for recoupling and allow for the prediction and design of potential new drugs.
Unravelling physiological disorders in onion and garlic: critical assessment and bibliometric visualization
Authors: Kale Rajiv B. , Khandagale Kiran , Ramadas Sendhil , Gavhane Abhishek Dilip , Gedam Pranjali , Mahajan Vijay
Published: December 2024

Onion and garlic are economically important vegetable crops cultivated worldwide. Numerous pests and diseases affect the quality and yield of these crops. In addition to diseases and pests, several physiological disorders affect onion and garlic. The physiological disorders are abnormalities caused by intercultural operations, nutrient management, environmental factors, genetic regulation, etc. These physiological disorders significantly affect the yield and quality of onion and garlic, leading to monetary losses to the farmers. The following physiological disorders are commonly reported in onion: premature bolting, sprouting in storage, watery scale, doubling/twins, basal plate split, and thick neck. Premature sprouting and rubberization are the main physiological anomalies observed in garlic. The present review described the symptoms of these physiological abnormalities, the factors responsible, and ways to avoid or minimise the occurrence of these abnormalities to subsequently reduce the losses of the growers. Further, we also performed bibliometric analysis using the SCOPUS database. This is the first review that describes the progress of research on physiological disorders in onion and garlic in detail, which will positively increase awareness about such important aspects of onion and garlic. Further, it will provide insight to researchers for developing innovative strategies, cultural practices, and varieties to control these physiological abnormalities of onion and garlic.
Critical review of aging clocks and factors that may influence the pace of aging
Authors: Min Mildred , Egli Caitlin , Dulai Ajay S. , Sivamani Raja K.
Published: December 2024

Background and objectives

Aging clocks are computational models designed to measure biological age and aging rate based on age-related markers including epigenetic, proteomic, and immunomic changes, gut and skin microbiota, among others. In this narrative review, we aim to discuss the currently available aging clocks, ranging from epigenetic aging clocks to visual skin aging clocks.

Methods

We performed a literature search on PubMed/MEDLINE databases with keywords including: “aging clock,” “aging,” “biological age,” “chronological age,” “epigenetic,” “proteomic,” “microbiome,” “telomere,” “metabolic,” “inflammation,” “glycomic,” “lifestyle,” “nutrition,” “diet,” “exercise,” “psychosocial,” and “technology.”

Results

Notably, several CpG regions, plasma proteins, inflammatory and immune biomarkers, microbiome shifts, neuroimaging changes, and visual skin aging parameters demonstrated roles in aging and aging clock predictions. Further analysis on the most predictive CpGs and biomarkers is warranted. Limitations of aging clocks include technical noise which may be corrected with additional statistical techniques, and the diversity and applicability of samples utilized.

Conclusion

Aging clocks have significant therapeutic potential to better understand aging and the influence of chronic inflammation and diseases in an expanding older population.
Assuring assistance to healthcare and medicine: Internet of Things, Artificial Intelligence, and Artificial Intelligence of Things
Authors: Belbase Poshan , Bhusal Rajan , Ghimire Sapana Sharma , Sharma Shreesti , Banskota Bibek
Published: December 2024

Introduction

The convergence of healthcare with the Internet of Things (IoT) and Artificial Intelligence (AI) is reshaping medical practice with promising enhanced data-driven insights, automated decision-making, and remote patient monitoring. It has the transformative potential of these technologies to revolutionize diagnosis, treatment, and patient care.

Purpose

This study aims to explore the integration of IoT and AI in healthcare, outlining their applications, benefits, challenges, and potential risks. By synthesizing existing literature, this study aims to provide insights into the current landscape of AI, IoT, and AIoT in healthcare, identify areas for future research and development, and establish a framework for the effective use of AI in health.

Method

A comprehensive literature review included indexed databases such as PubMed/Medline, Scopus, and Google Scholar. Key search terms related to IoT, AI, healthcare, and medicine were employed to identify relevant studies. Papers were screened based on their relevance to the specified themes, and eventually, a selected number of papers were methodically chosen for this review.

Results

The integration of IoT and AI in healthcare offers significant advancements, including remote patient monitoring, personalized medicine, and operational efficiency. Wearable sensors, cloud-based data storage, and AI-driven algorithms enable real-time data collection, disease diagnosis, and treatment planning. However, challenges such as data privacy, algorithmic bias, and regulatory compliance must be addressed to ensure responsible deployment of these technologies.

Conclusion

Integrating IoT and AI in healthcare holds immense promise for improving patient outcomes and optimizing healthcare delivery. Despite challenges such as data privacy concerns and algorithmic biases, the transformative potential of these technologies cannot be overstated. Clear governance frameworks, transparent AI decision-making processes, and ethical considerations are essential to mitigate risks and harness the full benefits of IoT and AI in healthcare.
Efficient dataset extension using generative networks for assessing degree of coating degradation around scribe
Authors: Stursa Dominik , Rozsival Pavel , Dolezel Petr
Published: December 2024

A novel methodology for dataset augmentation in the semantic segmentation of coil-coated surface degradation is presented in this study. Deep convolutional generative adversarial networks (DCGAN) are employed to generate synthetic input-target pairs, which closely resemble real-world data, with the goal of expanding an existing dataset. These augmented datasets are used to train two state-of-the-art models, U-net, and DeepLabV3, for the precise detection of degradation areas around scribes. In a series of experiments, it was demonstrated that the introduction of synthetic data improves the models' performance in detecting degradation, especially when the ratio of synthetic to real data is carefully managed. Results indicate that optimal improvements in accuracy and F1-score are achieved when the ratio of synthetic to original data is between 0.2 and 0.5. Moreover, the advantages and limitations of different GAN architectures for dataset expansion are explored, with specific attention to their ability to produce realistic and diverse samples. This work offers a scalable solution to the challenges associated with creating large and diverse annotated datasets for industrial applications of coil coating degradation assessment. The proposed approach provides a significant contribution by improving model generalization and segmentation accuracy while reducing the burden of manual data annotation. These findings have important implications for industries relying on coil coatings, as more efficient and accurate degradation detection methods are enabled.
Vaccine sero-monitoring and sero-surveillance of Peste des petits ruminants in small ruminants in West Gojjam zone, Amhara region, Ethiopia
Authors: Mandefro Mesafint , Ibrahim Saddam Mohammed , Sibhatu Demeke , Kassa Nebiyou , Emiyu Kemal , Debebe Kebede , Dessalegn Bereket , Birhan Mastewal , Bitew Molalegne
Published: December 2024

Background

Peste des petits ruminants (PPR) is an acute or subacute, highly contagious, and economically important, transboundary disease of small ruminants caused by Peste des petits ruminants virus (PPRV).

Objectives

The objective of this study was to determine the seroconversion rate in PPR vaccinated flock of sheep (Sekela district) and the seroprevalence of PPRV in unvaccinated flocks of sheep and goats (Yilmanadensa district).

Methods

A cross-sectional study was conducted from January to March 2022 in two selected districts of West Gojjam zone, Ethiopia. Multistage cluster sampling was used to select sampling units by successively selecting districts, kebeles, and villages purposively based on their accessibility and vaccination status. Individual animals were selected haphazardly mimicking simple random sampling. Accordingly, a total of 660 blood samples were collected. Out of this, 300 sheep were vaccinated 4 months prior to sampling using the Nigerian 75/1 strain-based freeze-dried live attenuated PPR vaccine and 360 small ruminants (288 sheep and 72 goats) were unvaccinated and assayed for anti-PPRV antibodies using commercial c-ELISA.

Results

The post-vaccination herd immunity was 76.66% (95% CI: 71.46–81.34), which is slightly lower than the threshold herd immunity recommended by the PPR global control and eradication strategy, which is set to be 80%, to efficiently break the epidemiological cycle of the virus. Seroprevalence of PPRV in unvaccinated sheep and goats was 3.61% (95% CI: 1.94–6.1), indicating the possible circulation of PPRV in the area. Although small ruminants develop solid immunity following natural infection in endemic countries, the infection of naïve animals allows continuous circulation of the virus.

Conclusions

In light of the accumulating evidence of low post-vaccination herd immunity in small ruminants in Ethiopia, the undergoing PPR vaccination strategy needs to be reevaluated to achieve the desired herd immunity at any time ultimately aiding the eradication goal by 2030.
Effects of corn oil ingestion on the intestinal mucosa of normal dogs
Authors: An Su-Jin , Kim Young Joo , Hong Il-Hwa , Jung Dong-In
Published: December 2024

Introduction

We assessed corn oil’s oral effectiveness in detecting small bowel changes in healthy dogs through ultrasonography, endoscopy, and histopathology. We hypothesize that corn oil ingestion will not significantly increase the visibility of lymphatics and lacteals in healthy dogs.

Methods

Five healthy male beagles were studied under institutional guidelines. The small intestine’s mucosal changes were observed post corn oil consumption (0.5 mL/kg) at various time intervals using ultrasonography, endoscopy, and histopathology. Ultrasonography was employed in real-time, and mucosal echogenicity scores were assigned at multiple time points. Endoscopic and capsule endoscopic examinations were conducted weekly at different intervals post oil consumption, with biopsy samples taken from the duodenal and ileal mucosa for histopathological evaluations.

Results

Ultrasonographic evaluations showed no pathological conditions in any dog. While conventional endoscopic evaluations reflected normal variation, capsule endoscopy revealed significant duodenal and jejunal mucosal changes 3 h post-ingestion, but not in the ileum. Histopathological evaluation indicated a transient rise in the dilation of ileum villi 3 h post-ingestion, reducing by 12 h.

Conclusion

In conclusion, this study demonstrated that the observed physiological changes in the small intestinal mucosa, including lymphatic dilation, hyperechoic speckles, and stripes, were within the normal range after oil ingestion in healthy Beagle dogs.
The effects of camel milk in systemic inflammation and oxidative stress of cigarette smoke-induced chronic obstructive pulmonary disease model in rat
Authors: Behrouz Sepide , Mohammadi Mahla , Sarir Hadi , Boskabady Mohammad Hossein
Published: December 2024

Background

The effects of camel milk in inflammation and systemic oxidative stress of cigarette smoke (CS)-induced chronic obstructive pulmonary disease (COPD) associated with small airway inflammation in rats were investigated.

Methods

35 male Wistar rats were randomly divided into five groups: (a) control, (b) CS-exposed rats, c and (d) CS-exposed rats treated with the 4 and 8 mL/kg camel milk, and (e) CS-exposed rats treated with 1 mg/kg dexamethasone.

Results

Total and differential WBC counts, serum level of TNF-α and malondialdehyde (MDA) level in serum and homogenized tissues of the heart, kidney, liver, and testicle were significantly increased, but catalase (CAT), superoxide dismutase (SOD) and thiol levels were significantly decreased in CS-exposed rats (p < 0.01 to p < 0.001). Treatment with dexamethasone and both doses of camel milk improved all measured variables compared to the COPD group (p < 0.05 to p < 0.001). The improvements of most variables in the treated group with high dose of camel milk were higher than the effect of dexamethasone (p < 0.05 to p < 0.001). These findings suggest that camel milk has a therapeutic potential for treating systemic oxidative stress and inflammatory induced by CS.

Conclusion

Therefore, camel milk might be effective in attenuating the effects of CS-induced systemic inflammation and oxidative stress.
A Multi-Strategy Improved Honey Badger Algorithm for Engineering Design Problems
Authors: Han Tao , Li Tingting , Liu Quanzeng , Huang Yourui , Song Hongping
Published: December 2024

A multi-strategy improved honey badger algorithm (MIHBA) is proposed to address the problem that the honey badger algorithm may fall into local optimum and premature convergence when dealing with complex optimization problems. By introducing Halton sequences to initialize the population, the diversity of the population is enhanced, and premature convergence is effectively avoided. The dynamic density factor of water waves is added to improve the search efficiency of the algorithm in the solution space. Lens opposition learning based on the principle of lens imaging is also introduced to enhance the ability of the algorithm to get rid of local optimums. MIHBA achieves the best ranking in 23 test functions and 4 engineering design problems. The improvement of this paper improves the convergence speed and accuracy of the algorithm, enhances the adaptability and solving ability of the algorithm to complex functions, and provides new ideas for solving complex engineering design problems.
Pollen Rain in a Semi-Arid Area of Northeastern Brazil: Pollen Diversity, Concentrations over Two Years and Their Relationship with Ecological Aspects
Authors: Magalhães-e-Silva Francisco Hilder , Santos Francisco de Assis Ribeiro dos
Published: December 2024

Pollen rain studies are rare in arid and semi-arid regions worldwide. Interpretations related to the dynamics of plant communities and possible paleoclimatic changes in these areas face significant limitations due to this lack of data. The global biome of Seasonally Dry Tropical Forests and Shrublands (SDTFS) is represented in Northeast Brazil by the caatinga, which is composed of xerophytic vegetation. This study aimed to generate information about the pollen rain in this area and to understand its relationship with species flowering, pollination syndromes, life forms, and climatic aspects. A caatinga area in Canudos, Bahia, Brazil (09°54′ S 39°07′ W), was selected for this purpose. Artificial pollen collectors were installed and exchanged monthly over two years for palynological analyses of the collected material, using standard palynological techniques. A total of 124 pollen types were identified, with approximately 8823 pollen grains deposited per cm2 over the two years. Several vegetation components were represented in the pollen rain, reflecting local plant diversity, life forms, and physiognomies. A positive relationship was observed between increased temperature and pollen production from trees and shrubs, and new pollen types were associated with indicator species of caatinga vegetation.
Groundcovers Improve Soil Properties in Woody Crops Under Semiarid Climate
Authors: Sastre Blanca , Antón-Iruela Omar , Moreno-Delafuente Ana , Navas Mariela J. , Marques Maria Jose , González-Canales Javier , Martín-Sanz Juan Pedro , Ramos Rubén , García-Díaz Andrés , Bienes Ramón
Published: December 2024

There is a worldwide need to enhance soil health, particularly in agricultural areas. Groundcovers are widely recognized sustainable land management (SLM) practices that improve soil health and provide climate benefits by sequestering atmospheric carbon. A paired-plots study was carried out in woody crops (17 sites, olive groves and vineyards) in a semiarid area of central Spain to measure soil parameter changes induced by different management practices in the medium term. The selection across different locations aimed to determine whether the impact of groundcovers was substantial enough to produce significant changes in the studied soil parameters, even when accounting for variations in soil types. Each site consisted of neighboring plots: One was managed with conventional tillage (CT). The other was managed with an alternative soil management practice: (1) spontaneous groundcovers (GC) or (2) no soil management (NM). Vegetation and soil parameters were measured in spring 2021. Despite the low aboveground biomass in GC (77 g m−2), this treatment improved soil organic carbon stock (+4.4 Mg ha−1), infiltration rate (+50%), and aggregate stability (+35%) compared to CT, but higher compaction along the profile was detected. NM only resulted in a better infiltration rate, with high soil compaction. Our study provides supplementary information to long-term studies, which may include soil biological parameters as soil health indicators and yield response. Outcomes of these soil assessments lend support to the implementation of agricultural policies that promote GC as a SLM practice, in order to extend this technique to woody crops.
Characteristics of Dissolved Organic Matter (DOM) Combined with As in Fe-Rich Red Soils of Tea Plantations in the Southern Anhui Province, East China
Authors: Yao Youru , Li Juying , Ma Kang , Zhang Jingyi , Lin Yuesheng , Tan Huarong , Yu Jia , Fang Fengman
Published: December 2024

Dissolved organic matter (DOM) is widely present in soil environments and plays a crucial role in controlling the morphology, environmental behavior, and hazards of arsenic (As) in soil. In the Fe-rich red soil of tea plantations, the decomposition of tea tree litter complicates DOM properties, leading to more uncertain interactions between DOM, Fe, and As. This study focused on three tea plantations in Huangshan City to investigate the contents of DOM, Fe, and As in surface red soils (Ferralsols) and establish their correlations. Three-dimensional fluorescence spectroscopy and PARAFAC analysis methods were used to analyze the DOM components and fluorescence signatures. Additionally, the process and mechanism of the binding of DOM-Fe with As were explored through laboratory experiments on the morphological transformation of As by DOM-Fe. The results showed that the pH values of the soils in the three tea plantations ranged from 3.9 to 5.2, and the entire sample was strongly acidic. The DOM exhibited strong intrinsic properties and low humification, containing three types of humic acid components and one intermediate protein component. The DOC content in the Fe-rich red soil did not have a direct correlation with Fe and As, but the interaction of DOM fractions with Fe significantly influenced the As content. Specifically, the interaction of protein-like fractions with Fe had a more pronounced effect on the As content. The maximum sorption rate of As by DOM was 15.45%, and this rate increased by 49 to 75% with the participation of Fe. In the configuration of the metal electron bridge, Fe acts as a cation, forming a connecting channel between the negatively charged DOM and As, thus enhancing the DOM’s binding capacity to As. DOM-Fe compounds bind As through surface pores and functional groups. These findings provide deeper insights into the influence of DOM on As behavior in Fe-rich soil environments and offer theoretical support for controlling As pollution in red soil.
Agronomic Evaluation of Wheat (Triticum aestivum L.) Under Different Degrees of Drought–Rehydration Conditions Under Drip Irrigation
Authors: Wang Rongrong , Kong Liting , Bie Shuting , Tu Hongming , Cai Jingyi , Jiang Guiying , Xu Jianwei
Published: December 2024

Establishing an optimal population structure is the fundamental approach to achieving high crop yield. By studying the changes in spring wheat yield and population structure under varying degrees of drought–rehydration conditions under drip irrigation, we can understand the balance between growth and stress response, explore the potential of wheat for biological water saving, and provide scientific evidence for the efficient production of drip-irrigated wheat in drought-prone areas. In this study, we used “Xinchun 6” (water-insensitive variety, XC 6) and “Xinchun 22” (water-sensitive variety, XC 22) as materials. Under two-year field planting conditions, mild (T1, J1, 60~65% FC, FC represents field capacity) and moderate (T2, J2, 45~50% FC) drought stress treatments were applied during the tillering and jointing stages, followed by drip irrigation for rehydration. The conventional drip irrigation served as the control (CK, 75~80% FC). We analyzed the relationship between the population quality and yield of different genotypes of wheat under water stress during the growth period and clarified the response of dry matter translocation to grains and high-quality populations to drought–rehydration. The results showed that drought stress reduced the tiller number (NT), leaf area index (LAI), grain number–leaf ratio (GNL), grain weight–leaf ratio (GWL), and dry matter weight. After rehydration, LAI, specific leaf weight (SLW), GNL, GWL, dry matter of vegetative organ and grain weight, and grain yield all reached their maximum values under T1 treatment. Compared with CK and moderate drought treatments (T2 and J2, respectively), these indicators under T1 treatment increased by an average of 1.04~30.96%, 0.82~6.28%, 0.57~26.10%, 0.41~8.01%, 0.48~41.10%, 0.53~13.97%, and 0.17~49.75%, respectively. Additionally, T1 treatment improved the post-flowering dry matter translocation rate and contribution rate. The compensatory effects on NT, LAI, GNL, GWL, and yield under drought–rehydration treatments during the tillering stage (T1 and T2) were superior to those during the jointing stage (J1 and J2). Correlation and path analysis indicated that yield was significantly positively correlated with LAI, GNL, and GWL, and increasing LAI had the best effect on yield increase. This suggests that rehydration after mild drought stress (T1) during the tillering stage can maintain a suitable leaf area for the population, enhance the grain–leaf ratio, promote post-anthesis material production and storage material transportation, coordinate the source–sink relationship, and achieve high yields for drip-irrigated spring wheat.
Hemp Cover Cropping and Disease Suppression in Winter Wheat of the Dryland Pacific Northwest
Authors: Hagerty Christina H. , Shrestha Govinda , Wen Nuan , Kroese Duncan R. , Namdar Grayson F. , Paulitz Tim , Wysocki Donald J.
Published: December 2024

The predominant cropping scheme for dryland wheat production in the Pacific Northwest (PNW) region of the United States includes winter wheat–summer fallow. Lack of crop diversification can deplete the soil organic matter and nutrients, while favoring the build-up of soilborne diseases. Cover crops are becoming more common within a standard rotation, primarily to provide protection against soil erosion, incorporate nutrients, and break soilborne diseases’ cycles. In this study, we investigated the potential of using hemp as a cover crop in a dryland wheat rotation to reduce soilborne diseases, and thus increase farmers’ profitability. While the benefits of barley and yellow mustard cover crops are well understood, the benefits of a hemp cover crop have not been examined in the PNW. We observed Fusarium spp. disease suppression on winter wheat following a hemp cover crop in the greenhouse studies. However, under field conditions, we did not observe a difference in pathogen abundance on winter wheat following hemp cover crop and hemp amendments in the field. Any potential to limit soilborne disease is a profitability opportunity for farmers. Our findings indicate that incorporating a hemp rotation into the PNW dryland wheat production system holds promise as a strategy to reduce soilborne diseases and improve soil health, though further research is necessary to confirm its effectiveness and underlying mechanism.
Effects of Malondialdehyde on Growth Performance, Gastrointestinal Health, and Muscle Quality of Striped Catfish (Pangasianodon hypophthalmus)
Authors: Peng Cong , Fu Xinlangji , Zhang Yumeng , Zhang Haitao , Ye Yuantu , Deng Junming , Tan Beiping
Published: December 2024

Malondialdehyde (MDA) is a reactive carbonyl compound produced through lipid peroxidation during feed storage, which poses a significant threat to fish health. This study aimed to evaluate the effects of dietary MDA on the growth rate, gastrointestinal health, and muscle quality of striped catfish (Pangasianodon hypophthalmus). A basal diet (M0) containing 34% crude protein and 10.5% crude lipid was formulated. Each group was sprayed with malondialdehyde solution (0, 5, 10, 20, 40, and 80 mg/kg, on dietary crude lipid basis; 0, 0.53, 1.07, 2.13, 4.26, and 8.52 mg/kg, on dietary basis) before feeding, respectively. Each diet was randomly assigned to triplicates of 30 striped catfish (initial weight 31.38 g) per net cage. After 8 weeks, dietary inclusion of MDA regardless of level significantly depressed the growth rate and feed utilization. The extent of structural damage to the gastrointestinal tract increased progressively with increasing dietary MDA levels. The extent of damage to the intestinal biological barrier (intestinal microbial structure), chemical barrier (trypsin, lipase, amylase, and maltase activity), physical barrier (zonula occludent-2, occludin, claudin 7α, and claudin 12 relative expression), and immune barrier (contents of complement 4, complement 3, immunoglobulin M, and lysozyme activity) was dose-related to dietary MDA. Moreover, a linear decline in the activities of intestinal antioxidant enzymes (catalas, superoxide dismutase, et al.) and anti-inflammatory factor (transforming growth factor beta1, interleukin 10) relative expression was noted alongside an increase in dietary MDA content. In contrast, the relative expression levels of intestinal inflammatory factor (interleukin 8, transcription factor p65, tumor necrosis factor alpha) relative expression displayed an opposing trend. Additionally, dietary MDA exerted a linear influence on muscle color and texture characteristics. In conclusion, high doses of MDA (5–80 mg/kg) reduced the growth performance of striped catfish, attributed to linear damage to the gastrointestinal tract, a linear decrease in antioxidant function, and the occurrence of an inflammatory response. High doses of MDA (>40 mg/kg) were observed to significantly increase dorsal muscle b-value and induce muscle yellowing.
Fullerenol C60(OH)36: Antioxidant, Cytoprotective, Anti-Influenza Virus Activity, and Self-Assembly in Aqueous Solutions and Cell Culture Media
Authors: Borisenkova Alina A. , Eropkin Mikhail Y. , Konovalova Nadezhda I. , Titova Anna V. , Markova Maria A. , Lyutova Zhanna B. , Mazur Anton S. , Sedov Victor P. , Orlova Vera A. , Lykholay Anna N. , Orlova Diana N. , Arutyunyan Alexandr V.
Published: December 2024

Viral infections and many other dangerous diseases are accompanied by the development of oxidative stress, which is a consequence of an increase in the level of the reactive oxygen species (ROS). In this regard, the search for effective antioxidants remains highly relevant. We tested fullerenol C60(OH)36 in the context of the connection between its self-assembly in aqueous solutions and cell culture media, antiradical activity, UV cytoprotective action, and antiviral activity against international reference strains of influenza virus A(H1N1)pdm09, A(H3N2), and B subtypes in vitro on the MDCK cell line. Various characterization techniques, including Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, NMR and ESR spectrometry, MALDI-TOF mass spectrometry, thermal analysis (TGA and DSC), dynamic light-scattering (DLS), and ζ-potential measurements, were used to confirm the production of fullerenol and study its self-assembly in aqueous solutions and cell culture media. Fullerenol C60(OH)36 demonstrated the ability to scavenge •DPPH, •OH, O2•− radicals and 1O2 and was non-toxic in the range of the studied concentrations (up to 200 μg/mL) when incubated with MDCK cells for 24 h. In addition, fullerenol exhibited a cytoprotective effect under UV irradiation (EC50 = 29.7 ± 1.0 μM) and showed moderate activity against human influenza viruses of subtypes A(H1N1)pdm09 (SI = 9.9 ± 4.6) and A(H3N2) (SI = 12.5 ± 1.3) when determined by the hemagglutination assay (HA-test) and the MTT assay. At the same time, C60(OH)36 was ineffective in vitro against the actual strain of influenza B virus (Victoria lineage). The high bioavailability of fullerenol in combination with its cytoprotective effect, as well as its antiradical and antiviral activity combined with a relatively low toxicity, allows to consider it a promising compound for biomedical applications.