bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Key Homomorphic Encryption Scheme with Multi-Output Programmable Bootstrapping
Authors: Li Lingwu , Huang Ruwei
Published: July 2023

Multi-key Homomorphic Encryption (MKHE) scheme can homomorphically evaluate ciphertexts encrypted by different keys, which can effectively protect the privacy information of data holders in the joint computing of cloud services. Since the first full Homomorphic encryption scheme was proposed, bootstrapping is the only way to realize the arbitrary depth homomorphic computation of MKHE schemes. But bootstrap operation is quite expensive. In order to implement fast bootstrapping in MKHE schemes, previous works proposed multi-key TFHE schemes to implement low-latency bootstrapping and output a univariate function of messages after bootstrapping, called Programmable Bootstrapping (PBS). However, these schemes can only encrypt single-bit messages. PBS only outputs a function. And after a homomorphic operation, a bootstrap is required, which undoubtedly results in an increase in the cost of the whole multi-key homomorphic encryption operation. In this paper, we propose a MKHE scheme for multi-output PBS. For this purpose, we study the encryption method and homomorphic operation steps of MKHE, and add BFV homomorphic encryption multiplication and multi-key ciphertext relinearization. We separate the homomorphic operation from bootstrapping. We homomorphically evaluate test polynomials for multiple functions. In contrast to previous MKHE schemes, we support the output of multiple message-related functions with a single bootstrapping operation on the ciphertext. It is no longer limited to encrypting single-bit plaintext, and an effective ciphertext packaging technology is added. According to the analysis given in this paper, it is known that in the scenario of multi-party joint computation, the proposed scheme can be implemented with less bootstrapping when the same number of functions are homomorphically operated. This will effectively reduce the computational overhead.
Optimal Multi-Attribute Auctions Based on Multi-Scale Loss Network
Authors: Zhao Zefeng , Cai Haohao , Ma Huawei , Zou Shujie , Chu Chiawei
Published: July 2023

There is a strong demand for multi-attribute auctions in real-world scenarios for non-price attributes that allow participants to express their preferences and the item’s value. However, this also makes it difficult to perform calculations with incomplete information, as a single attribute—price—no longer determines the revenue. At the same time, the mechanism must satisfy individual rationality (IR) and incentive compatibility (IC). This paper proposes an innovative dual network to solve these problems. A shared MLP module is constructed to extract bidder features, and multiple-scale loss is used to determine network status and update. The method was tested on real and extended cases, showing that the approach effectively improves the auctioneer’s revenue without compromising the bidder.
A New Neurological Screening Approach for Diagnosing Brainstem Infarction Using the Calling Method and Familiar Voices
Authors: Ohshiro Yuzuru
Published: July 2023

This report proposes a new approach to assess dysarthria in patients with brainstem infarction by involving familiar individuals. Collaboration provides valuable insights compared to subjective traditional methods. A man in his 70s presented with resolved positional vertigo. Standard neurological tests showed no abnormalities, and inquiries with the patient’s friend did not reveal voice changes. While inquiring about voice changes with family, friends, and acquaintances is a common practice in clinical settings, our approach involved the patient calling out to his friend from a distance. Despite the physician detecting no abnormalities, the friend noticed a lower voice. Subsequent magnetic resonance imaging (MRI) confirmed brainstem infarction. Early and subtle symptoms of brainstem infarction pose a detection challenge and can lead to serious outcomes if overlooked. This report provides the first evidence that distance calling can detect subtle voice changes associated with brainstem infarction potentially overlooked by conventional neurological examinations, including inquiries with individuals familiar with the patient’s voice. Detecting brainstem infarction in emergency department cases is often missed, but conducting MRIs on every patient is not feasible. This simple method may identify patients overlooked by conventional screening who should undergo neuroimaging such as MRI. Further research is needed, and involving non-professionals in assessments could significantly advance the diagnostic process.
Persistent Pancytopenia as a Long-COVID Manifestation in a Patient with Adult-Onset Still’s Disease: A Case Report
Authors: Massimino Mattia , Iaquinta Francesco Salvatore , Naty Saverio , Andreozzi Francesco , Grembiale Rosa Daniela
Published: July 2023

Background: Adult-onset Still’s disease (AOSD) is a rare rheumatic inflammatory condition with an extremely heterogeneous clinical presentation and systemic impairment. Uncommon manifestations may be challenging to manage, especially in patients with previous severe acute SARS-CoV-2 infection. For the first time, we report the case of a patient affected by refractory AOSD presenting with severe pancytopenia as a long-COVID manifestation. The purpose of this case report is to illustrate the clinical presentation, diagnostic and therapeutic management of this unusual manifestation. Moreover, we examine the mechanisms that are potentially responsible for the onset of the pancytopenia observed in our patient. Case presentation: We describe the case of a 40-year-old male who presented with a history of fever for 2 years, arthralgia, maculopapular salmon-pink rash and a previous SARS-CoV-2 infection which required admission to intensive care. The patient’s laboratory results revealed elevated inflammatory markers levels (erythrocyte sedimentation rate and C-reactive protein), hyperferritinemia and severe pancytopenia that needed multiple transfusions. A diagnosis of AOSD was made based on clinical and laboratory presentation after excluding neoplastic, infectious and other rheumatic diseases. The previous empirical treatment was not adequate to control the condition; therefore, treatment with high-dose steroids, canakinumab and epoetin alfa was started and led to the resolution of the man’s symptoms and a reduction in inflammatory marker levels, whereas blood cell count remained stable without a need for further blood transfusions. The patient is currently under rheumatologic and hematologic follow-up every month. Conclusions: Neither AOSD nor SARS-CoV-2 infection usually manifests with pancytopenia, except in hemophagocytic syndrome or immunodeficient patients, respectively. Identifying the underlying etiology of pancytopenia is mandatory to establish a prompt treatment that generally resolves the disorder. However, in our case, all common causes of pancytopenia were excluded, suggesting a potential manifestation of the long-COVID syndrome. Despite the resolution of the acute infection and the remarkable treatment of AOSD, pancytopenia persists. Herein, we propose for refractory AOSD patients with previous SARS-CoV-2 infection a novel approach to the diagnosis and treatment of pancytopenia.
Anti-Obesity Effect of a Tea Mixture Nano-Formulation on Rats Occurs via the Upregulation of AMP-Activated Protein Kinase/Sirtuin-1/Glucose Transporter Type 4 and Peroxisome Proliferator-Activated Receptor Gamma Pathways
Authors: Salem Mohamed A. , Aborehab Nora M. , Abdelhafez Mai M. , Ismail Sameh H. , Maurice Nadine W. , Azzam May A. , Alseekh Saleh , Fernie Alisdair R. , Salama Maha M. , Ezzat Shahira M.
Published: July 2023

White, green, and oolong teas are produced from the tea plant (Camellia sinensis (L.) Kuntze) and are reported to have anti-obesity and hypolipidemic effects. The current study aims to investigate the anti-obesity effects of a tea mixture nano-formulation by targeting the AMPK/Sirt-1/GLUT-4 axis in rats. In vitro lipase and α-amylase inhibition assays were used to determine the active sample, which was then incorporated into a nanoparticle formulation subjected to in vivo anti-obesity testing in rats by measuring the expression level of different genes implicated in adipogenesis and inflammation using qRT-PCR. Moreover, metabolomic analysis was performed for each tea extract using LC/ESI MS/MS coupled to chemometrics in an attempt to find a correlation between the constituents of the extracts and their biological activity. The in vitro pancreatic lipase and α-amylase inhibition assays demonstrated more effective activity in the tea mixture than the standards, orlistat and acarbose, respectively, and each tea alone. Thus, the herbal tea mixture and its nanoparticle formulation were evaluated for their in vivo anti-obesity activity. Intriguingly, the tea mixture significantly decreased the serum levels of glucose and triglycerides and increased the mRNA expression of GLUT-4, P-AMPK, Sirt-1, and PPAR-γ, which induce lipolysis while also decreasing the mRNA expression of TNF-α and ADD1/SREBP-1c, thereby inhibiting the inflammation associated with obesity. Our study suggests that the tea mixture nano-formulation is a promising therapeutic agent in the treatment of obesity and may also be beneficial in other metabolic disorders by targeting the AMPK/Sirt-1/Glut-4 pathway.
Metabolite Profiling in a Diet-Induced Obesity Mouse Model and Individuals with Diabetes: A Combined Mass Spectrometry and Proton Nuclear Magnetic Resonance Spectroscopy Study
Authors: Vieira João P. P. , Ottosson Filip , Jujic Amra , Denisov Vladimir , Magnusson Martin , Melander Olle , Duarte João M. N.
Published: July 2023

Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy techniques have been used extensively for metabolite profiling. Although combining these two analytical modalities has the potential of enhancing metabolite coverage, such studies are sparse. In this study we test the hypothesis that combining the metabolic information obtained using liquid chromatography (LC) MS and 1H NMR spectroscopy improves the discrimination of metabolic disease development. We induced metabolic syndrome in male mice using a high-fat diet (HFD) exposure and performed LC-MS and NMR spectroscopy on plasma samples collected after 1 and 8 weeks of dietary intervention. In an orthogonal projection to latent structures (OPLS) analysis, we observed that combining MS and NMR was stronger than each analytical method alone at determining effects of both HFD feeding and time-on-diet. We then tested our metabolomics approach on plasma from 56 individuals from the Malmö Diet and Cancer Study (MDCS) cohort. All metabolic pathways impacted by HFD feeding in mice were confirmed to be affected by diabetes in the MDCS cohort, and most prominent HFD-induced metabolite concentration changes in mice were also associated with metabolic syndrome parameters in humans. The main drivers of metabolic disease discrimination emanating from the present study included plasma levels of xanthine, hippurate, 2-hydroxyisovalerate, S-adenosylhomocysteine and dimethylguanidino valeric acid. In conclusion, our combined NMR-MS approach provided a snapshot of metabolic imbalances in humans and a mouse model, which was improved over employment of each analytical method alone.
Bacterial Flora on Mist Outlet Surfaces in 4D Theaters and Suspended Particle Concentration Characteristics during 4D Movie Screenings
Authors: Yanagi U , Kaihara Noriko , Simazaki Dai , Bekki Kanae , Homma Yoshinori , Iba Chiemi , Asai Atsuto , Hayashi Motoya
Published: July 2023

In this study, we measured suspended particle concentrations during the screening of 4D movies (3 screens and 15 movies) and 2D movies (9 screens and 9 movies) in 3 movie theaters to obtain a more detailed understanding of the situation of suspended particle concentrations and adherent bacterial flora in 4D movie theaters, which have been introduced in increasing numbers in recent years. The adherent bacterial flora on the floor and mist outlet surfaces in the 4D movie theaters were collected and analyzed. During the movie showings, the concentrations of suspended particles in 4D movie theaters were significantly higher than those in 2D movie theaters (p < 0.001). A significant increase in suspended particle concentrations due to 4D movie effects was also observed. The results of the α-diversity and β-diversity analyses indicate that the bacterial flora on the surfaces of mist outlets in 4D movie theaters are similar. Moreover, there are many closely related species, and the bacterial flora are rich and contain rare bacterial species. Many of the bacterial genera that are dominant in 4D theaters are suited to aqueous environments, and bacteria in the water supply system may have an impact on the indoor environment.
Access to 2-Fluorinated Aziridine-2-phosphonates from α,α-Halofluorinated β-Iminophosphonates—Spectroscopic and Theoretical Studies
Authors: Klarek Mateusz , Siodła Tomasz , Ayad Tahar , Virieux David , Rapp Magdalena
Published: July 2023

The efficient one-pot halofluorination of a β-enaminophosphonate/β-iminophosphonate tautomeric mixture resulting in α,α-halofluorinated β-iminophosphonates is reported. Subsequent imine reduction gave the corresponding β-aminophosphonates as a racemic mixture or with high diastereoselectivity. The proposed protocol is the first example of a synthesis of N-inactivated aziridines substituted by a fluorine and phosphonate moiety on the same carbon atom. Based on spectroscopic and theoretical studies, we determined the cis/trans geometry of the resulting fluorinated aziridine-2-phosphonate. Our procedure, involving the reduction of cis/trans-fluoroaziridine mixture 24, allows us to isolate chiral trans-aziridines 24 as well as cis-aziridines 27 that do not contain a fluorine atom. We also investigated the influence of the fluorine atom on the reactivity of aziridine through an acid-catalyzed regioselective ring-opening reaction. The results of DFT calculations, at the PCM/ωB97x-D/def2-TZVPD level of theory, are in good agreement with the experiments. The transition states of the SN2 intramolecular cyclization of vicinal haloamines have been modeled.
Molecular Dynamics Simulations on the Adsorbed Monolayers of N-Dodecyl Betaine at the Air–Water Interface
Authors: Zhang Chengfeng , Cao Lulu , Jiang Yongkang , Huang Zhiyao , Liu Guokui , Wei Yaoyao , Xia Qiying
Published: July 2023

Betaine is a kind of zwitterionic surfactant with both positive and negative charge groups on the polar head, showing good surface activity and aggregation behaviors. The interfacial adsorption, structures and properties of n-dodecyl betaine (NDB) at different surface coverages at the air–water interface are studied through molecular dynamics (MD) simulations. Interactions between the polar heads and water molecules, the distribution of water molecules around polar heads, the tilt angle of the NDB molecule, polar head and tail chain with respect to the surface normal, the conformations and lengths of the tail chain, and the interfacial thickness of the NDB monolayer are analyzed. The change of surface coverage hardly affects the locations and spatial distributions of the water molecules around the polar heads. As more NDB molecules are adsorbed at the air–water interface, the number of hydrogen bonds between polar heads and water molecules slightly decreases, while the lifetimes of hydrogen bonds become larger. With the increase in surface coverage, less gauche defects along the alkyl chain and longer NDB chain are obtained. The thickness of the NDB monolayer also increases. At large surface coverages, tilted angles of the polar head, tail chain and whole NDB molecule show little change with the increase in surface area. Surface coverages can change the tendency of polar heads and the tail chain for the surface normal.
The Preparation of Superhydrophobic Polylactic Acid Membrane with Adjustable Pore Size by Freeze Solidification Phase Separation Method for Oil–Water Separation
Authors: Zhang Yan , Sun Tianyi , Zhang Dashuai , Sun Shishu , Liu Jinrui , Li Bangsen , Shi Zaifeng
Published: July 2023

An environmentally friendly pore size-controlled, superhydrophobic polylactic acid (PLA) membrane was successfully prepared by a simpler freeze solidification phase separation method (FSPS) and solution impregnation, which has application prospects in the field of oil–water separation. The pore size and structure of the membrane were adjusted by different solvent ratios and solution impregnation ratios. The PLA-FSPS membrane after solution impregnation (S-PLA-FSPS) had the characteristics of uniform pore size, superhydrophobicity and super lipophilicity, its surface roughness Ra was 338 nm, and the contact angle to water was 151°. The S-PLA-FSPS membrane was used for the oil–water separation. The membrane oil flux reached 16,084 L·m−2·h−1, and the water separation efficiency was 99.7%, which was much higher than that of other oil–water separation materials. In addition, the S-PLA-FSPS membrane could also be applied for the adsorption and removal of oil slicks and underwater heavy oil. The S-PLA-FSPS membrane has great application potential in the field of oil–water separation.
Structural Characterization and Molecular Model Construction of High-Ash Coal from Northern China
Authors: Zhu Benkang , Dong Xianshu , Fan Yuping , Ma Xiaomin , Yao Suling , Fu Yuanpeng , Chen Ruxia , Chang Ming
Published: July 2023

High-ash coal, also known as low-grade coal, has becomes a viable alternative in recent years to high-quality coal because available resources have become increasingly scarce due to extensive mining activity. This work aims to provide a comprehensive understanding of the structural characteristics of high-ash coal and construct a plausible molecular structure to elucidate its chemical reactivity in future applications. Its properties were investigated using Solid-state 13C nuclear magnetic resonance (13C NMR), X-ray photoelectron spectroscopy analysis (XPS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). The molecular structure was constructed and validated using Material Studio, LAMMPS Software Package, and MATLAB program. The characterization results revealed that high-ash coal contains 72.15% aromatic carbon, significantly surpassing the percentage of aliphatic carbon (27.85%). The ratio of bridgehead carbon to peripheral aromatic carbon was calculated as 0.67, indicating that the pentacene is the main carbon skeleton form in the high-ash coal structure. Furthermore, oxygen-containing functional groups presented as C=O/O–C–O, C–O, and COO– within the structure along with pyridine and pyrrolic structures. Consequently, the molecular structure comprises pentacene with aliphatic carbon chains, such as methylene, that connect the benzene rings and form a three-dimensional network. The results of a simulated IR spectrum and contact angle simulation aligned with the experimental results, validating the molecular structure of high-ash coal. The chemical formula for the high-ash coal model was determined as C203H189N7O61S with a molecular weight of 3734.79.
Explaining Multiclass Compound Activity Predictions Using Counterfactuals and Shapley Values
Authors: Lamens Alec , Bajorath Jürgen
Published: July 2023

Most machine learning (ML) models produce black box predictions that are difficult, if not impossible, to understand. In pharmaceutical research, black box predictions work against the acceptance of ML models for guiding experimental work. Hence, there is increasing interest in approaches for explainable ML, which is a part of explainable artificial intelligence (XAI), to better understand prediction outcomes. Herein, we have devised a test system for the rationalization of multiclass compound activity prediction models that combines two approaches from XAI for feature relevance or importance analysis, including counterfactuals (CFs) and Shapley additive explanations (SHAP). For compounds with different single- and dual-target activities, we identified small compound modifications that induce feature changes inverting class label predictions. In combination with feature mapping, CFs and SHAP value calculations provide chemically intuitive explanations for model decisions.
Microbiological Survey and Antimicrobial Resistance of Foodborne Bacteria in Select Meat Products and Ethnic Food Products Procured from Food Desert Retail Outlets in Central Virginia, USA
Authors: Kim Chyer , Goodwyn Brian , Albukhaytan Sakinah , Nartea Theresa , Ndegwa Eunice , Dhakal Ramesh
Published: July 2023

In food desert areas, low-income households without convenient transportation often shop at small, independently owned corner markets and convenience stores (SIOMs). Studies indicate a higher potential for reduced product quality and safety of foods sold at SIOMs, with more critical and non-critical code violations in the region. This study aimed to assess the difference in market scale on the microbiological quality in select food products procured from food deserts in Central Virginia. A total of 326 samples consisting of meat products (i.e., ground beef, chicken, and sausage), ethnic food products (i.e., ox tail, stock fish bite, egusi ground, and saffron powder), and food packaging surfaces procured from ten registered SIOMs and nine large chain supermarkets (LCSMs) between August 2018 and March 2020 were evaluated. Higher levels of aerobic mesophile and coliform counts were found in SIOMs-acquired samples than in LCSMs-acquired samples, as demonstrated by the lower food safety compliance rate of SIOMs. Regardless of SIOMs or LCSMs, Campylobacter, E. coli, Listeria, and Salmonella were detected in 3.6%, 20.9%, 5.5%, and 2.7% of samples, respectively. The majorities of Campylobacter (75%, 6/8) and Salmonella (83.3%, 5/6) detected were from SIOMs-acquired samples including ethnic food products. Among the tested antimicrobials, AMP (100%) and TOB (100%) showed the highest frequency of resistance among Campylobacter, TCY (69.9%) among E. coli, NAL (100%) among Listeria, and TCY (50%) among Salmonella, respectively. The prevalence of multi-drug resistance (MDR) and non-susceptibility in Campylobacter and non-susceptibility in Listeria isolated from SIOMs-acquired food products were lower than those isolated from LCSMs-acquired samples. A higher price of the same brand name commodity sold at SIOMs than those sold at LCSMs was also observed, indicating an increased financial burden to economically challenged residents in food desert areas, in addition to food safety concerns. Elaborated and in-depth research on a larger-scale sample size with a greater diversity of products is needed to determine and intervene in the cause(s) of the observed differences in the prevalence of the pathogens and AMR profiles.
Montjuïc Hill (Barcelona): A Hotspot for Plant Invasions in a Mediterranean City
Authors: Ibáñez Neus , Gómez-Bellver Carlos , Farelo Paula , Montserrat Josep Maria , Pyke Samuel , Nualart Neus , López-Pujol Jordi
Published: July 2023

Cities are often hotspots for biological invasions, showing much higher percentages of alien species than non-urbanized settings. The reasons are multiple and are mostly related to two main factors: their heterogeneous, highly disturbed habitats and their many gateways that allow alien species introduction (e.g., airports, roads, train stations, or gardens). In addition to being a sink of biological invasions, cities can also be a source of the spread of alien species into surrounding landscapes, which adds further complexity to this issue. Herein, we are presenting the results of a five-year survey of the alien flora of Montjuïc, the largest urban hill in Barcelona (Spain). In just about 3.4 km2, we recorded up to 247 alien plant taxa, a figure much higher than those of many other Mediterranean cities and which clearly points to the role of Montjuïc as a hotspot for alien plants. The comparison with the alien flora of its surrounding region (coastal Catalonia) suggests that the alien flora of Montjuïc would have become enriched through many immigration episodes from close geographic areas. The hill, however, would have also acted as a source of the spread of alien plants, and indeed, some species have not been detected yet beyond the confines of Montjuïc. This study aims to be a key tool to ensure early detection and also to develop appropriate management and/or eradication actions.
Climatic Niche Dynamics of the Astereae Lineage and Haplopappus Species Distribution following Amphitropical Long-Distance Dispersal
Authors: Rosas Marcelo R. , Segovia Ricardo A. , Guerrero Pablo C.
Published: July 2023

The tribe Astereae (Asteraceae) displays an American Amphitropical Disjunction. To understand the eco-evolutionary dynamics associated with a long-distance dispersal event and subsequent colonization of extratropical South America, we compared the climatic and geographic distributions of South American species with their closest North American relatives, focusing on the diverse South American Astereae genus, Haplopappus. Phylogenetic analysis revealed that two South American genera are closely related to seven North American genera. The climatic niche overlap (D = 0.5) between South and North America exhibits high stability (0.89), low expansion (0.12), and very low unfilling (0.04). The distribution of the North American species predicted the climatic and geographic space occupied by the South American species. In central Chile, Haplopappus showed a non-random latitudinal gradient in species richness, with Mediterranean climate variables mainly explaining the variation. Altitudinal patterns indicated peak richness at 600 m, declining at lower and higher elevations. These findings support climatic niche conservatism in shaping Haplopappus species distribution and diversity. Two major endemism zones were identified in central Chile and the southern region, with a transitional zone between Mediterranean and Temperate macro-bioclimates. Our results indicate strong niche conservatism following long-distance dispersal and slight niche expansion due to unique climatic variables in each hemisphere.
45S rDNA Diversity In Natura as One Step towards Ribosomal Heterogeneity in Arabidopsis thaliana
Authors: Delorme-Hinoux Valérie , Mbodj Assane , Brando Sophie , De Bures Anne , Llauro Christel , Covato Fabrice , Garrigue Joseph , Guisset Claude , Borrut Jacques , Mirouze Marie , Reichheld Jean-Philippe , Sáez-Vásquez Julio
Published: July 2023

The keystone of ribosome biogenesis is the transcription of 45S rDNA. The Arabidopsis thaliana genome contains hundreds of 45S rDNA units; however, they are not all transcribed. Notably, 45S rDNA units contain insertions/deletions revealing the existence of heterogeneous rRNA genes and, likely, heterogeneous ribosomes for rRNAs. In order to obtain an overall picture of 45S rDNA diversity sustaining the synthesis of rRNAs and, subsequently, of ribosomes in natura, we took advantage of 320 new occurrences of Arabidopsis thaliana as a metapopulation named At66, sampled from 0 to 1900 m of altitude in the eastern Pyrenees in France. We found that the 45S rDNA copy number is very dynamic in natura and identified new genotypes for both 5′ and 3′ External Transcribed Spacers (ETS). Interestingly, the highest 5′ETS genotype diversity is found in altitude while the highest 3′ETS genotype diversity is found at sea level. Structural analysis of 45S rDNA also shows conservation in natura of specific 5′ETS and 3′ETS sequences/features required to control rDNA expression and the processing of rRNAs. In conclusion, At66 is a worthwhile natural laboratory, and unraveled 45S rDNA diversity represents an interesting starting material to select subsets for rDNA transcription and alter the rRNA composition of ribosomes both intra- and inter-site.
Efficacy of Aspergillus tubingensis GX3′ Fermentation against Meloidogyne enterolobii in Tomato (Solanum lycopersicum L.)
Authors: Sikandar Aatika , Gao Fukun , Mo Yixue , Chen Qian , Ullah Rana Muhammad Kaleem , Wu Haiyan
Published: July 2023

Meloidogyne enterolobii is one of the most virulent root-knot nematodes (RKNs). Aspergillus tubingensis Raoul Mosseray, 1934, is used to produce bioactive substances, enzymes, and secondary metabolites. However, no research has been conducted yet on the efficacy of A. tubingensis against plant-parasitic nematodes. Thus, the novel research was planned to evaluate the biocontrol efficacy of A. tubingensis fermentation against M. enterolobii. The findings showed that egg hatching inhibition and mortality of M. enterolobii increased with increasing concentration of fermentation and exposure time. The maximum second-stage juveniles (J2s) mortality was achieved via 100% fermentation at 72 h. Similarly, 100% fermentation inhibited 99.9% of egg hatching at 8 d. A. tubingensis fermentation increased plant biomass, decreased second-stage juvenile invasion, and inhibited nematode development and reproduction in greenhouse conditions. A. tubingensis reduced J2 invasion into tomato roots by 42.84% with CS+ (coated seeds plants with nematodes inoculum) and 27.04% with T+ (100% fermentation broth and nematodes inoculum both) treatments. Moreover, CS+ and T+ treatments decreased nematode development by 54.31% and 21.48%, respectively. It is concluded that the A. tubingensis GX3 strain can be used as a novel microbial biocontrol agent against M. enterolobii.
Transcriptome Analysis Reveals the Response Mechanism of Digitaria sanguinalis, Arabidopsis thaliana and Poa annua under 4,8-Dihydroxy-1-tetralone Treatment
Authors: Sun Qiumin , Wang Tao , Huang Jiu , Gu Xinyi , Dong Yanling , Yang Ying , Da Xiaowen , Mo Xiaorong , Xie Xiaoting , Jiang Hangjin , Yan Daoliang , Zheng Bingsong , He Yi
Published: July 2023

4,8-dihydroxy-l-tetralone (4,8-DHT) is an allelochemical isolated from the outer bark of Carya cathayensis that acts as a plant growth inhibitor. In order to explore the mechanism of 4,8-DHT inhibiting weed activity, we treated three species of Digitaria sanguinalis, Arabidopsis thaliana, and Poa annua with different concentrations of 4,8-DHT and performed phenotype observation and transcriptome sequencing. The results showed that with an increase in 4,8-DHT concentration, the degree of plant damage gradually deepened. Under the same concentration of 4,8-DHT, the damage degree of leaves and roots of Digitaria sanguinalis was the greatest, followed by Arabidopsis thaliana, while Poa annua had the least damage, and the leaves turned slightly yellow. Transcriptome data showed that 24536, 9913, and 1662 differentially expressed genes (DEGs) were identified in Digitaria sanguinalis, Arabidopsis thaliana, and Poa annua, respectively. These DEGs were significantly enriched in photosynthesis, carbon fixation, glutathione metabolism, phenylpropanoid biosynthesis, and oxidative phosphorylation pathways. In addition, DEGs were also enriched in plant hormone signal transduction and the MAPK signal pathway in Arabidopsis thaliana. Further analysis showed that after 4,8-DHT treatment, the transcript levels of photosynthesis PSI- and PSII-related genes, LHCA/B-related genes, Rubisco, and PEPC were significantly decreased in Digitaria sanguinalis and Arabidopsis thaliana. At the same time, the transcription levels of genes related to glutathione metabolism and the phenylpropanoid biosynthesis pathway in Digitaria sanguinalis were also significantly decreased. However, the expression of these genes was upregulated in Arabidopsis thaliana and Poa annua. These indicated that 4,8-DHT affected the growth of the three plants through different physiological pathways, and then played a role in inhibiting plant growth. Simultaneously, the extent to which plants were affected depended on the tested plants and the content of 4,8-DHT. The identification of weed genes that respond to 4,8-DHT has helped us to further understand the inhibition of plant growth by allelochemicals and has provided a scientific basis for the development of allelochemicals as herbicides.
Metabolomic Characterization of Phoradendron brachystachyum Mistletoe and In-Silico and In-Vitro Investigation of Its Therapeutic Potential in Metabolic Disorders
Authors: Montoya-Inzunza Luis Aurelio , Moreno-Ulloa Aldo , Carballo-Castañeda Rommel A. , Xool-Tamayo Jorge , Contreras-Angulo Laura Aracely , Leyva-López Nayely , Antunes-Ricardo Marilena , Gonzalez-Galaviz Jose Reyes , Heredia José Basilio , Gutiérrez-Grijalva Erick Paul
Published: July 2023

Plants of the Phoradendron genus have been traditionally used for their lipid- and glucose-lowering effects. However, the compounds responsible for these effects and the overall chemical profile of these plants have not been thoroughly investigated. We aimed to characterize the metabolome of leaves, stems, and aerial parts of the Phoradendron brachystachyum plant. We used mass spectrometry and colorimetric screening techniques (with various solvents) to identify and characterize the metabolites present. We also evaluated the antioxidant (FRAP, ORAC, TEAC, and DPPH assays) and inhibitory effects on pancreatic lipase and α-glucosidase enzymes of hydrophilic extracts. Furthermore, we compared the molecular fingerprints between the identified metabolites and FDA-approved drugs to gain insights into the metabolites that might be responsible for the observed effects on enzymes. Our findings revealed the presence of 59 putative metabolites, primarily flavonoids. However, we also hint at the presence of peptide and carbohydrate derivatives. The leaf extracts demonstrated the most promising metrics across all assays, exhibiting strong antioxidant and enzyme inhibitory effects as well as high levels of phenolic compounds, flavonoids, and tannins. Fingerprint analysis suggested potential peptide and carbohydrate metabolites as pancreatic lipase and α-glucosidase inhibitors. Overall, our study provides evidence on specific metabolites in Phoradendron brachystachyum that could be responsible for the therapeutic effects noted in obese and type 2 diabetes subjects.
Application of Near-Infrared Reflectance Spectroscopy for Predicting Damage Severity in a Diverse Panel of Tectona grandis Caused by Ceratocystis fimbriata
Authors: Anjos Isabela Vera dos , Ali Mohsin , Mora-Poblete Freddy , Araujo Kelly Lana , Gilio Thiago Alexandre Santana , Neves Leonarda Grillo
Published: July 2023

Tectona grandis Linn., also known as teak, is a highly valued species with adaptability to a wide range of climatic conditions and high tolerance to soil variations, making it an attractive option for both commercial and conservation purposes. In this sense, the classification of cultivated teak genotypes is crucial for both breeding programs and conservation efforts. This study examined the relationship between traits related to damage in the stem of teak plants caused by Ceratocystis fimbriata (a soil-borne pathogen that negatively impacts the productivity of teak plantations) and the spectral reflectance of 110 diverse clones, using near-infrared spectroscopy (NIRS) data and partial least squares regression (PLSR) analysis. Cross-validation models had R2 = 0.894 (ratio of standard error of prediction to standard deviation: RPD = 3.1), R2 = 0.883 (RPD = 2.7), and R2 = 0.893 (RPD = 2.8) for predicting stem lesion area, lesion length, and severity of infection, respectively. Teak genotypes (clones) can benefit from the creation of a calibration model utilizing NIRS-generated data paired with PLSR, which can effectively screen the magnitude of damage caused by the fungus. Overall, while the study provides valuable information for teak breeding and conservation efforts, a long-term perspective would be essential to evaluate the sustainability of teak genotypes over various growth stages and under continuous pathogen pressure.
Physical Properties and Molecular Interactions Applied to Food Processing and Formulation
Authors: Polachini Tiago Carregari , Morales Sergio Andres Villalba , Filho Luís Roberto Peixoto , Ribeiro Elisa Franco , Saraiva Larissa Santos , Basso Rodrigo Corrêa
Published: July 2023

Food processes have been requiring increasingly more accurately designed operations. Successful design results in products with high quality, in addition to offering energy and cost savings. To these ends, fundamental knowledge regarding the physical properties and thermodynamic mechanisms of the material is essential. The aim of this review was to highlight important concepts and applications of some thermophysical properties (density, specific heat, thermal conductivity and thermal diffusivity), as well as recent methods for their determination. The rheology of fluids and solids is widely discussed according to the concepts, classification, modeling and applications involved in food and equipment design. Herein, we report destructive and non-destructive assays for the evaluation of food properties. Due to the complexity of food systems, the effects of modifications of the structure and physicochemical reactions on the quality of the resulting food are addressed based on thermodynamic aspects. This analytical perspective was adopted in food systems rich in fats, proteins, polysaccharides and simple sugars. Using the information reported in this study, formulations and unit operations can be better designed. In addition, process failures become more predictable when fundamental knowledge is available. Therefore, food losses can be avoided, quality can be maintained and operations can be effectively resumed when deviations from ideal conditions are evidenced.
Study on Oxy-Methane Flame Stability in a Cylindrical Porous Medium Burner
Authors: Liao Mingjian , He Zhu , Liang Xiong , Chan Tat Leung , Li Yawei , Xu Xuecheng
Published: July 2023

Combustion in a porous medium can be beneficial for enhancing reaction rate and temperature uniformity. Therefore, considering the combination with oxy-fuel combustion can address some shortcomings in oxy-fuel burners, a cylindrical two-layer porous burner model is established based on OpenFOAM in this paper. A two-temperature equation model is adopted for the simulation of the heat transfer process. The CH4 skeletal kinetic mechanism is adopted for complex chemistry integration based on OpenSMOKE++. Corresponding experimental methods were used for complementary studies. The walls of the burner are wrapped with three types of thermal insulation materials to present different levels of heat loss. The results show that considering the convection and radiative heat loss of the burner wall, the temperature near the wall is reduced by more than 300 K compared to the adiabatic condition. As a result, the flame propagation speed and CO oxidation rate slowed down. The stable range will be destructively narrowed by more than 50%, and CO emissions will increase by more than 10 times. These defects will be aggravated by increasing the diameter of the burner. It is observed that when the diameter of the burner increases from the initial 5 cm to 10 cm, the effect of heat loss on the stable range is insignificant.
Review of the Research on and Optimization of the Flow Force of Hydraulic Spool Valves
Authors: Li Ruichuan , Sun Yuhang , Wu Xiaowei , Zhang Peng , Li Defang , Lin Jianghai , Xia Yuhai , Sun Qiyou
Published: July 2023

As one of the important factors affecting the stability of slide valves, the analysis and research of flow force are of great significance. In recent years, more and more experts and scholars have conducted research in this field, attempting to find methods to reduce or utilize the flow force of hydraulic spool valves. Flow force includes steady-state flow force and transient flow force, with steady-state flow force having the most significant impact on spool valves. The influencing factors of flow force are complex and diverse, including the cavitation phenomenon, shape of the throttling groove, and jet angle. At present, the main ways to reduce flow force are to design the structure of the spool valve, the structure of the valve sleeve, and the flow channel of the valve body. This article mainly reviews the definition, calculation methods, influencing factors, and methods for reducing the flow force of slide valves. This provides a new approach to reducing the flow force in hydraulic spool valves.
The Evaluation Technology of Manufacturer Intelligence Regarding the Selection of the Decision Support System of Smart Manufacturing Technologies: Analysis of China–South Africa Relations
Authors: Pei Fengque , Zhang Jiaxuan , Yuan Minghai , He Fei , Yan Bingwen
Published: July 2023

With the development of international cooperation, South Africa (SA) has been China’s largest trading partner in Africa for several consecutive years. China and SA can build the digital “Belt and Road” to modernize the manufacturing system locally and optimize process control by benchmarking with the best-in-class manufacturers in each country. In this research, an evaluation technology of manufacturer intelligence regarding the selection of decision support system (DSS) of smart manufacturing technologies, analyzing China–South Africa relations, is described. Firstly, the three keys aspects that enable the technologies of DSS are discussed in detail. Then, one key technology, the manufacturers’ intelligent evaluation system with 15 indexes, was built. The indexes and their measurements are also proposed. Finally, a fusion method based on boosting with multi-kernel function (online sequential extreme learning machine based on boosting, Boosting-OSELM) is introduced. The purpose of Boosting-OSKELM is to combine several weak learners into a strong learner (lower mean square error, MSE) through an acceptable time delay. Finally, the case study is presented to demonstrate the improvement on the MSE and process time, showing a relative MSE improvement of 96.19% and a relative time delay ratio of 31.46%. Totally, the largest contribution of the proposed evaluation method in this study is the conversion of the history data saved by the manual scoring method into knowledge in accessible MES and resealable time delay, which will free up the expert workforce in the entire process. We expect this paper will help future research in this field.
Sludge Reduction and Surface Investigation in Electrochemical Machining by Complexing and Reducing Agents
Authors: Cercal Gustavo , de Alvarenga Gabriela , Vidotti Marcio
Published: July 2023

Electrochemical machining (ECM) is widely applied to manufacture parts with complex geometries, used in electronic components and the automotive, military, and aeronautics industries. These parts have a surface shaped by controlled anodic dissolution at high current density levels, using a neutral solution of inorganic salts (i.e., NaCl or NaNO3) as the electrolyte. Such conditions generate a high amount of sludge that deposits onto the surfaces of equipment, devices, cathodes, and working pieces, requiring daily and complicated sludge management during the series production in the industry. Thus, the main goal of the present work is to propose a simple way to reduce sludge generation in the ECM industrial process. To do so, complexing (EDTA) or reducing (ascorbic acid) agents were added to the electrolyte composition, creating parallel reactions to keep the metallic ions from precipitating. The complexing agent EDTA resulted in a 30% reduction in sludge mass, using an alkaline solution (pH > 10.0). The reducing agent, ascorbic acid, resulted in a 90% reduction in sludge mass, using an acidic solution (pH < 5.0). This sludge reduction has the potential to contribute significantly to increasing equipment, devices, and cathode lifetime, as well as reducing costs associated with centrifuge or filter maintenance (sludge removal from electrolyte) and increasing the productivity of industrial ECM processes.
The Effect of Cu2+ and Pb2+ in the Feed Solution on the Water and Reverse Solute Fluxes in the Forward Osmosis (FO) Process Using Nanofiltration (NF) Membranes
Authors: Atashgar Amirsajad , Emadzadeh Daryoush , Kruczek Boguslaw
Published: July 2023

The application of nanofiltration (NF) membranes in the forward osmosis (FO) process to remove heavy metal ions from wastewater is an emerging concept. Unlike NF, FO does not require an external driving force. Although the product, a dilute draw solution, must further be processed by NF to produce pure water and reconcentrate a draw solution, the feed to that NF process is “clean”, which minimizes membrane fouling. This paper examines the role of Cu2+ and Pb2+ in the feed solution on the water and the reverse solute fluxes in FO process using novel thin film nanocomposite (TFN) NF membranes. The TFN membranes were fabricated by in situ interfacial polymerization of piperazine (PIP) and 1,3,5-benzenetricarbonyl trichloride (TMC) containing different amounts of dispersed halloysite nanotubes (HNTs) nanoparticles functionalized with the first generation of poly(amidoamine) (PAMAM) dendrimers. The presence of Cu2+ and Pb2+ in the feed solution decreased the reverse flux of MgCl2 by at least 2.5 times compared to the experiments with pure water as a feed. Simultaneously, the water flux also increased. The corresponding rejections of Cu2+ and Pb2+ in the FO process ranged from 94.5% to 98.1%.
Mass-Integration and Environmental Evaluation of Chitosan Microbeads Production Modified with of Thiourea and Magnetite Nanoparticles
Authors: González-Delgado Ángel Darío , Cogollo-Cárcamo Grisel , Bertel-Pérez Forlin
Published: July 2023

Bioadsorbents based on biopolymers modified with magnetic nanoparticles stand out for being non-toxic, effective, and easy to recover. Thus, the objective of the present work was to carry out a computer-aided environmental evaluation of the industrial-scale production of bioadsorbents from chitosan modified with iron nanoparticles and functionalized with thiourea as a chelating agent plus mass integration. The plant simulation was carried out in Aspen Plus, and for the mass integration of the process, a pinch analysis was used to determine the minimum target fresh and residual water amount, assuming two process stages: (1) the synthesis of magnetite nanoparticles, and (2) the production of chitosan-based bioadsorbents. The environmental assessment was performed using the waste reduction algorithm (WAR). The potential environmental impact (PEI) was quantified, taking into account the impact of the products and energy, obtaining a value below zero of −0.78 PEI/kg of the product. The photochemical oxidation potential (PCOP) stood out as the category with the greatest impact, mainly related to the use of ethanol during washing. The categories related to toxicological impacts (HTPI, HTPE, TTP, and ATP) had lower values than those related to atmospheric impacts (GWP, ODP, PCOP, and AP). The mass integration of the process resulted in fewer impacts in the HTPE category, as a consequence of the decrease in NaOH in the residual streams and the release of 0.297 PEI/kg of product in the ATP category caused by the presence of Al(OH)₃. It can be concluded that the mass integration managed to reduce up to 51% of the freshwater used in the processes, and it is a useful tool, as it slightly decreased the total potential impacts.
Advances in Energy System Synthesis and the Energy–Water Nexus in Industry
Authors: Wang Yufei , Lee Jui-Yuan , Zhang Haoran
Published: July 2023

Energy and water are key resources for human life and industry production [...]