bluuebunny/crossref_metadata_2025 huggingface dataset:
The Appointment System Influences Uptake of Cataract Surgical Services in Rwanda
Authors: Kitema Gatera Fiston , Morjaria Priya , Mathenge Wanjiku , Ramke Jacqueline
Published: January 2021

The aim of this study was to investigate barriers and enablers associated with the uptake of cataract surgery in Rwanda, where financial protection is almost universally available. This was a hospital-based cross-sectional study where potential participants were adults aged >18 years who accepted an appointment for cataract surgery during the study period (May–July 2019). Information was collected from hospital records and a semi-structured questionnaire was used for data collection. Of the 297 people with surgery appointments, 221 (74.4%) were recruited into the study, 126 (57.0%) of whom had attended their appointment. People more likely to attend their surgical appointment were literate, had fewer than 8 children, had poorer visual acuity, had access to a telephone in the family, received a specific date to attend their appointment, received a reminder, and reported no difficulties walking (95% significance level, p < 0.05). The most commonly reported barriers were insufficient information about the appointment (n = 40/68, 58.8%) and prohibitive indirect costs (n = 29/68, 42.6%). This study suggests that clear communication of appointment information and a subsequent reminder, together with additional support for people with limited mobility, are strategies that could improve uptake of cataract surgery in Rwanda.
Personal and Environmental Risk Factors at Birth and Hospital Admission: Direct and Vitamin D-Mediated Effects on Bronchiolitis Hospitalization in Italian Children
Authors: Zaffanello Marco , Ferrante Giuliana , Fasola Salvatore , Piazza Michele , Piacentini Giorgio , La Grutta Stefania
Published: January 2021

Seasonal variations in UV-B radiation may influence vitamin D status, and this, in turn, may influence the risk of bronchiolitis hospitalization. The aim of this study was using a causal inference approach to investigate, simultaneously, the interrelationships between personal and environmental risk factors at birth/hospital admission (RFBH), serum vitamin D levels and bronchiolitis hospitalization. A total of 63 children (<2 years old) hospitalized for bronchiolitis (34 RSV-positive) and 63 controls were consecutively enrolled (2014–2016). Vitamin D levels and some RFBH (birth season, birth weight, gestational age, gender, age, weight, hospitalization season) were recorded. The discovered RFBH effects on the risk ok bronchiolitis hospitalization were decomposed into direct and vitamin-D mediated ones through Mediation Analysis. Winter-spring season (vs. summer-autumn) was significantly associated with lower vitamin D levels (mean difference −11.14 nmol/L). Increasing serum vitamin D levels were significantly associated with a lower risk of bronchiolitis hospitalization (OR = 0.84 for a 10-nmol/L increase). Winter-spring season and gestational age (one-week increase) were significantly and directly associated with bronchiolitis hospitalization (OR = 6.37 and OR = 0.78 respectively), while vitamin D-mediated effects were negligible (1.21 and 1.02 respectively). Using a comprehensive causal approach may enhance the understanding of the complex interrelationships among RFBH, vitamin D and bronchiolitis hospitalization.
Exploring the Effects of Problematic Internet Use on Adolescent Sleep: A Systematic Review
Authors: Kokka Ioulia , Mourikis Iraklis , Nicolaides Nicolas C. , Darviri Christina , Chrousos George P. , Kanaka-Gantenbein Christina , Bacopoulou Flora
Published: January 2021

Adolescent suse internet via several devices to gather information or communicate. Sleep, as a key factor of adolescents’ development, contributes to their physical and mental health. Over the past decades insufficient sleep among adolescents has been wide spread, and one of its attributing factors is the increased availability of technology. This review aims to investigate the body of evidence regarding the impact of problematic internet use on adolescent sleep. Extensive search of databases was performed according to PRISMA guidelines for studies published within the last decade, regarding subjects aged 10–19. The final step of the search yielded 12 original studies. The quality of extracted data was evaluated with the AXIS tool, in order to estimate the risk of bias. All studies showed a negative correlation between adolescent sleep and problematic internet use. It was found to affect sleep quality and quantity and provoke insomnia symptoms. Interestingly, adolescent’s sex, parental educational level, type of family and use for leisure or academic reasons appeared as affecting factors of the problematic internet use-sleep relationship. Problematic internet use has several effects on adolescents’ sleep. Results of relevant studies should be embedded in educational interventions addressed to adolescents as well as parents, to eliminate the negative outcomes of problematic internet use on sleep and adolescence’s health in general.
Characterization and Function of the 1-Deoxy-D-xylose-5-Phosphate Synthase (DXS) Gene Related to Terpenoid Synthesis in Pinus massoniana
Authors: Li Rong , Chen Peizhen , Zhu Lingzhi , Wu Fan , Chen Yu , Zhu Peihuang , Ji Kongshu
Published: January 2021

In the methyl-D-erythritol-4-phosphate (MEP) pathway, 1-deoxy-D-xylose-5-phosphate synthase (DXS) is considered the key enzyme for the biosynthesis of terpenoids. In this study, PmDXS (MK970590) was isolated from Pinus massoniana. Bioinformatics analysis revealed homology of MK970590 with DXS proteins from other species. Relative expression analysis suggested that PmDXS expression was higher in roots than in other plant parts, and the treatment of P. massoniana seedlings with mechanical injury via 15% polyethylene glycol 6000, 10 mM H2O2, 50 μM ethephon (ETH), 10 mM methyl jasmonate (MeJA), and 1 mM salicylic acid (SA) resulted in an increased expression of PmDXS. pET28a-PmDXS was expressed in Escherichia coli TransB (DE3) cells, and stress analysis showed that the recombinant protein was involved in resistance to NaCl and drought stresses. The subcellular localization of PmDXS was in the chloroplast. We also cloned a full-length 1024 bp PmDXS promoter. GUS expression was observed in Nicotiana benthamiana roots, stems, and leaves. PmDXS overexpression significantly increased carotenoid, chlorophyll a, and chlorophyll b contents and DXS enzyme activity, suggesting that DXS is important in isoprenoid biosynthesis. This study provides a theoretical basis for molecular breeding for terpene synthesis regulation and resistance.
Description of AtCAX4 in Response to Abiotic Stress in Arabidopsis
Authors: Bu Yuanyuan , Fu Weichao , Chen Jiangpo , Takano Tetsuo , Liu Shenkui
Published: January 2021

High-capacity tonoplast cation/H+ antiport in plants is partially mediated by a family of CAX transporters. Previous studies have reported that CAX activity is affected by an N-terminal autoinhibitory region. CAXs may be present as heterodimers in plant cells, and this phenomenon necessitates further study. In this study, we demonstrate that there is an interaction between CAX4 and CAX1 as determined by the use of a yeast two-hybrid system and a bimolecular fluorescence complementation assay. More specifically, the N-terminal of CAX4 interacts with CAX1. We further observed the over-expression and either a single or double mutant of CAX1 and CAX4 in response to abiotic stress in Arabidopsis. These results suggest that CAX1 and CAX4 can interact to form a heterodimer, and the N-terminal regions of CAX4 play important roles in vivo; this may provide a foundation for a deep study of CAX4 function in the future.
Pulling the Brakes on Fast and Furious Multiple Drug-Resistant (MDR) Bacteria
Authors: Khan Abid , Manzoor Khanzadi , Sultan Aamir , Saeed Maria , Rafique Mahrukh , Noushad Sameen , Talib Ayesha , Rentschler Simone , Deigner Hans-Peter
Published: January 2021

Life-threatening bacterial infections have been managed by antibiotics for years and have significantly improved the wellbeing and lifetime of humans. However, bacteria have always been one step ahead by inactivating the antimicrobial agent chemically or by producing certain enzymes. The alarming universal occurrence of multidrug-resistant (MDR) bacteria has compelled researchers to find alternative treatments for MDR infections. This is a menace where conventional chemotherapies are no longer promising, but several novel approaches could help. Our current review article discusses the novel approaches that can combat MDR bacteria: starting off with potential nanoparticles (NPs) that efficiently interact with microorganisms causing fatal changes in the morphology and structure of these cells; nanophotothermal therapy using inorganic NPs like AuNPs to destroy pathogenic bacterial cells; bacteriophage therapy against which bacteria develop less resistance; combination drugs that act on dissimilar targets in distinctive pathways; probiotics therapy by the secretion of antibacterial chemicals; blockage of quorum sensing signals stopping bacterial colonization, and vaccination against resistant bacterial strains along with virulence factors. All these techniques show us a promising future in the fight against MDR bacteria, which remains the greatest challenge in public health care.
Regulation of Kv11.1 Isoform Expression by Polyadenylate Binding Protein Nuclear 1
Authors: Stump Matthew R. , Nguyen Rachel T. , Drgastin Rachel H. , Search Delaney , Gong Qiuming , Zhou Zhengfeng
Published: January 2021

The Kv11.1 voltage-gated potassium channel, encoded by the KCNH2 gene, conducts the rapidly activating delayed rectifier current in the heart. KCNH2 pre-mRNA undergoes alternative polyadenylation to generate two C-terminal Kv11.1 isoforms in the heart. Utilization of a poly(A) signal in exon 15 produces the full-length, functional Kv11.1a isoform, while intron 9 polyadenylation generates the C-terminally truncated, nonfunctional Kv11.1a-USO isoform. The relative expression of Kv11.1a and Kv11.1a-USO isoforms plays an important role in the regulation of Kv11.1 channel function. In this study, we tested the hypothesis that the RNA polyadenylate binding protein nuclear 1 (PABPN1) interacts with a unique 22 nt adenosine stretch adjacent to the intron 9 poly(A) signal and regulates KCNH2 pre-mRNA alternative polyadenylation and the relative expression of Kv11.1a C-terminal isoforms. We showed that PABPN1 inhibited intron 9 poly(A) activity using luciferase reporter assays, tandem poly(A) reporter assays, and RNA pulldown assays. We also showed that PABPN1 increased the relative expression level of the functional Kv11.1a isoform using RNase protection assays, immunoblot analyses, and patch clamp recordings. Our present findings suggest a novel role for the RNA-binding protein PABPN1 in the regulation of functional and nonfunctional Kv11.1 isoform expression.
Possible Role of Metformin as an Immune Modulator in the Tumor Microenvironment of Ovarian Cancer
Authors: Tsogas Faye K. , Majerczyk Daniel , Hart Peter C.
Published: January 2021

Growing evidence suggests that the immune component of the tumor microenvironment (TME) may be highly involved in the progression of high-grade serous ovarian cancer (HGSOC), as an immunosuppressive TME is associated with worse patient outcomes. Due to the poor prognosis of HGSOC, new therapeutic strategies targeting the TME may provide a potential path forward for preventing disease progression to improve patient survival. One such postulated approach is the repurposing of the type 2 diabetes medication, metformin, which has shown promise in reducing HGSOC tumor progression in retrospective epidemiological analyses and through numerous preclinical studies. Despite its potential utility in treating HGSOC, and that the immune TME is considered as a key factor in the disease’s progression, little data has definitively shown the ability of metformin to target this component of the TME. In this brief review, we provide a summary of the current understanding of the effects of metformin on leukocyte function in ovarian cancer and, coupled with data from other related disease states, posit the potential mechanisms by which the drug may enhance the anti-tumorigenic effects of immune cells to improve HGSOC patient survival.
Emerging Roles of Protease-Activated Receptors (PARs) in the Modulation of Synaptic Transmission and Plasticity
Authors: Price Rachel , Mercuri Nicola Biagio , Ledonne Ada
Published: January 2021

Protease-activated receptors (PARs) are a class of G protein-coupled receptors (GPCRs) with a unique mechanism of activation, prompted by a proteolytic cleavage in their N-terminal domain that uncovers a tethered ligand, which binds and stimulates the same receptor. PARs subtypes (PAR1-4) have well-documented roles in coagulation, hemostasis, and inflammation, and have been deeply investigated for their function in cellular survival/degeneration, while their roles in the brain in physiological conditions remain less appreciated. Here, we describe PARs’ effects in the modulation of neurotransmission and synaptic plasticity. Available evidence, mainly concerning PAR1-mediated and PAR2-mediated regulation of glutamatergic and GABAergic transmission, supports that PARs are important modulators of synaptic efficacy and plasticity in normal conditions.
Isolation and Characterization of Compounds from Glycyrrhiza uralensis as Therapeutic Agents for the Muscle Disorders
Authors: Lee Eun Ju , Shaikh Sibhghatulla , Ahmad Khurshid , Ahmad Syed Sayeed , Lim Jeong Ho , Park Soyoung , Yang Hye Jin , Cho Won-Kyung , Park Sang-Joon , Lee Yong-Ho , Park So-Young , Ma Jin-Yeul , Choi Inho
Published: January 2021

Skeletal muscle is the most abundant tissue and constitutes about 40% of total body mass. Herein, we report that crude water extract (CWE) of G. uralensis enhanced myoblast proliferation and differentiation. Pretreatment of mice with the CWE of G. uralensis prior to cardiotoxin-induced muscle injury was found to enhance muscle regeneration by inducing myogenic gene expression and downregulating myostatin expression. Furthermore, this extract reduced nitrotyrosine protein levels and atrophy-related gene expression. Of the five different fractions of the CWE of G. uralensis obtained, the ethyl acetate (EtOAc) fraction more significantly enhanced myoblast proliferation and differentiation than the other fractions. Ten bioactive compounds were isolated from the EtOAc fraction and characterized by GC-MS and NMR. Of these compounds (4-hydroxybenzoic acid, liquiritigenin, (R)-(-)-vestitol, isoliquiritigenin, medicarpin, tetrahydroxymethoxychalcone, licochalcone B, liquiritin, liquiritinapioside, and ononin), liquiritigenin, tetrahydroxymethoxychalcone, and licochalcone B were found to enhance myoblast proliferation and differentiation, and myofiber diameters in injured muscles were wider with the liquiritigenin than the non-treated one. Computational analysis showed these compounds are non-toxic and possess good drug-likeness properties. These findings suggest that G. uralensis-extracted components might be useful therapeutic agents for the management of muscle-associated diseases.
NMR-Based Structural Characterization of a Two-Disulfide-Bonded Analogue of the FXIIIa Inhibitor Tridegin: New Insights into Structure–Activity Relationships
Authors: Schmitz Thomas , Paul George Ajay Abisheck , Nubbemeyer Britta , Bäuml Charlotte A. , Steinmetzer Torsten , Ohlenschläger Oliver , Biswas Arijit , Imhof Diana
Published: January 2021

The saliva of blood-sucking leeches contains a plethora of anticoagulant substances. One of these compounds derived from Haementeria ghilianii, the 66mer three-disulfide-bonded peptide tridegin, specifically inhibits the blood coagulation factor FXIIIa. Tridegin represents a potential tool for antithrombotic and thrombolytic therapy. We recently synthesized two-disulfide-bonded tridegin variants, which retained their inhibitory potential. For further lead optimization, however, structure information is required. We thus analyzed the structure of a two-disulfide-bonded tridegin isomer by solution 2D NMR spectroscopy in a combinatory approach with subsequent MD simulations. The isomer was studied using two fragments, i.e., the disulfide-bonded N-terminal (Lys1–Cys37) and the flexible C-terminal part (Arg38–Glu66), which allowed for a simplified, label-free NMR-structure elucidation of the 66mer peptide. The structural information was subsequently used in molecular modeling and docking studies to provide insights into the structure–activity relationships. The present study will prospectively support the development of anticoagulant-therapy-relevant compounds targeting FXIIIa.
Marginal Adaptation and Microbial Leakage at Conometric Prosthetic Connections for Implant-Supported Single Crowns: An In Vitro Investigation
Authors: Gehrke Peter , Hartjen Philip , Smeets Ralf , Gosau Martin , Peters Ulrike , Beikler Thomas , Fischer Carsten , Stolzer Carolin , Geis-Gerstorfer Jürgen , Weigl Paul , Schäfer Sogand
Published: January 2021

Encouraging clinical results were reported on a novel cone-in-cone coupling for the fixation of dental implant-supported crowns (Acuris, Dentsply Sirona Implants, Mölndal, Sweden). However, the presence or absence of a microgap and a potential bacterial leakage at the conometric joint has not yet been investigated. A misfit and a resulting gap between the conometric components could potentially serve as a bacterial reservoir that promotes plaque formation, which in turn may lead to inflammation of the peri-implant tissues. Thus, a two-fold study set-up was designed in order to evaluate the bidirectional translocation of bacteria along conometrically seated single crowns. On conometric abutments filled with a culture suspension of anaerobic bacteria, the corresponding titanium nitride-coated (TiN) caps were fixed by friction. Each system was sterilized and immersed in culture medium to provide an optimal environment for microbial growth. Positive and negative controls were prepared. Specimens were stored in an anaerobic workstation, and total and viable bacterial counts were determined. Every 48 h, samples were taken from the reaction tubes to inoculate blood agar plates and to isolate bacterial DNA for quantification using qrt-PCR. In addition, one Acuris test system was subjected to scanning electron microscopy (SEM) to evaluate the precision of fit of the conometric coupling and marginal crown opening. Throughout the observational period of one week, blood agar plates of the specimens showed no viable bacterial growth. qrt-PCR, likewise, yielded a result approaching zero with an amount of about 0.53 × 10−4 µg/mL DNA. While the luting gap/marginal opening between the TiN-cap and the ceramic crown was within the clinically acceptable range, the SEM analysis failed to identify a measurable microgap at the cone-in-cone junction. Within the limits of the in-vitro study it can be concluded that the Acuris conometric interface does not allow for bacterial translocation under non-dynamic loading conditions.
Gene Methylation and Silencing of WIF1 Is a Frequent Genetic Abnormality in Mantle Cell Lymphoma
Authors: Alshareef Abdulraheem , Peters Anthea C. , Gélébart Pascal , Chen Will , Lai Raymond
Published: January 2021

We have previously shown that the Wnt canonical pathway (WCP) is constitutively active in most cases of mantle cell lymphoma (MCL). Here, we aimed to elucidate the mechanisms underlying this biochemical deregulation. We hypothesized that gene methylation/silencing of WIF1 (Wnt inhibitory factor-1), a physiologic inhibitor of WCP, contributes to the deregulation of WCP and promotes cell growth in MCL. In support of this hypothesis, we found that the expression of WIF1 was detectable in none of the 4 MCL cell lines, and in only 2 of 5 tumors (40%) examined. Using methylation-specific PCR, we found evidence of gene methylation of WIF1 in 4 of 5 cell lines (80%) and in 24 of 29 (82%) tumors. The addition of the demethylation agent 5-aza-2′-deoxycytidine to Mino and JeKo-1, two WIF1-negative cell lines, restored the expression of WIF1 mRNA in these cells. Gene transfection of WIF1 into JeKo-1 and Mino cells significantly reduced cell growth, and this finding correlated with substantial downregulations of various proteins in WCP, such as β-catenin and pGSK-3β. In conclusion, our results support the concept that gene methylation/silencing of WIF1 is a frequent event in MCL, and this abnormality contributes to the aberrant activation of WCP. These results have provided further evidence that aberrant Wnt signaling is pathogenetically important in MCL and it may represent a potential therapeutic target.
Molecular Changes Underlying Hypertrophic Scarring Following Burns Involve Specific Deregulations at All Wound Healing Stages (Inflammation, Proliferation and Maturation)
Authors: Čoma Matúš , Fröhlichová Lucia , Urban Lukáš , Zajíček Robert , Urban Tomáš , Szabo Pavol , Novák Štěpán , Fetissov Vitaly , Dvořánková Barbora , Smetana Karel , Gál Peter
Published: January 2021

Excessive connective tissue accumulation, a hallmark of hypertrophic scaring, results in progressive deterioration of the structure and function of organs. It can also be seen during tumor growth and other fibroproliferative disorders. These processes result from a wide spectrum of cross-talks between mesenchymal, epithelial and inflammatory/immune cells that have not yet been fully understood. In the present review, we aimed to describe the molecular features of fibroblasts and their interactions with immune and epithelial cells and extracellular matrix. We also compared different types of fibroblasts and their roles in skin repair and regeneration following burn injury. In summary, here we briefly review molecular changes underlying hypertrophic scarring following burns throughout all basic wound healing stages, i.e. during inflammation, proliferation and maturation.
Systematic Literature Review of Security Pattern Research
Authors: Washizaki Hironori , Xia Tian , Kamata Natsumi , Fukazawa Yoshiaki , Kanuka Hideyuki , Kato Takehisa , Yoshino Masayuki , Okubo Takao , Ogata Shinpei , Kaiya Haruhiko , Hazeyama Atsuo , Tanaka Takafumi , Yoshioka Nobukazu , Priyalakshmi G.
Published: January 2021

Security patterns encompass security-related issues in secure software system development and operations that often appear in certain contexts. Since the late 1990s, about 500 security patterns have been proposed. Although the technical components are well investigated, the direction, overall picture, and barriers to implementation are not. Here, a systematic literature review of 240 papers is used to devise a taxonomy for security pattern research. Our taxonomy and the survey results should improve communications among practitioners and researchers, standardize the terminology, and increase the effectiveness of security patterns.
Landscape Planning of Infrastructure through Focus Points’ Clustering Analysis. Case Study: Plastiras Artificial Lake (Greece)
Authors: Sargentis G.-Fivos , Ioannidis Romanos , Iliopoulou Theano , Dimitriadis Panayiotis , Koutsoyiannis Demetris
Published: January 2021

Even though landscape quality is largely a subjective issue, the integration of infrastructure into landscapes has been identified as a key element of sustainability. In a spatial planning context, the landscape impacts that are generated by infrastructures are commonly quantified through visibility analysis. In this study, we develop a new method of visibility analysis and apply it in a case study of a reservoir (Plastiras dam in Greece). The methodology combines common visibility analysis with a stochastic tool for visual-impacts evaluation; points that generate high visual contrasts in landscapes are considered Focus Points (FPs) and their clustering in landscapes is analyzed trying to answer two questions: (1) How does the clustering of Focus Points (FPs) impact the aesthetic value of the landscape? (2) How can the visual impacts of these FPs be evaluated? Visual clustering is calculated utilizing a stochastic analysis of generated Zones of Theoretical Visibility. Based on the results, we argue that if the visual effect of groups of FPs is positive, then the optimal sitting of FPs should be in the direction of faint clustering, whereas if the effect is negative, the optimal sitting of FPs should be directed to intense clustering. In order to optimize the landscape integration of infrastructure, this method could be a useful analytical tool for environmental impact assessment or a monitoring tool for a project’s managing authorities. This is demonstrated through the case study of Plastiras’ reservoir, where the clustering of positively perceived FPs is found to be an overlooked attribute of its perception as a highly sustainable infrastructure project.
Distribution Patterns of Grasshoppers and Their Kin over the Eurasian Steppes
Authors: Sergeev Michael G.
Published: January 2021

The main aims of this paper are to reveal general patterns of Orthoptera distribution in the Eurasian steppes, to evaluate long-term trends of changes in distribution of taxa and populations, and to estimate the potential for population changes relative to human activity and global warming trends. The main publications concerning diversity and distribution of these insects over the steppes are analyzed. The fauna of the Eurasian steppes includes more than 440 species of Orthoptera. The general distribution of grasshoppers and their kin in the Eurasian steppes reflects their common associations with different grasslands. The species richness increases from the relatively cold forest-steppes to the semi-deserts with their warm summer. There are some endemic or subendemic taxa, including the tribe Onconotini (Tettigoniidae). The populations’ distribution of Orthoptera is also analyzed. The populations of native Orthoptera extend through all the herbaceous landscapes. Under these conditions, the interrelating of colonies of each species may result in great abundance. The population distribution of three species locusts (Locusta migratoria, Calliptamus italicus, Dociostaurus maroccanus) is also discussed. Some notable changes of their populations’ distribution and dynamics are characterized. The situation with rare Orthoptera is estimated. Retrospective and prospective of the steppe fauna of Orthoptera are discussed.
The Myrmecofauna (Hymenoptera: Formicidae) of Hungary: Survey of Ant Species with an Annotated Synonymic Inventory
Authors: Csősz Sándor , Báthori Ferenc , Gallé László , Lőrinczi Gábor , Maák István , Tartally András , Kovács Éva , Somogyi Anna Ágnes , Markó Bálint
Published: January 2021

Ants (Hymenoptera: Forimicidae) are exceedingly common in nature. They constitute a conspicuous part of the terrestrial animal biomass and are also considered common ecosystem engineers. Due to their key role in natural habitats, they are at the basis of any nature conservation policy. Thus, the first step in developing adequate conservation and management policies is to build a precise faunistic inventory. More than 16,000 valid ant species are registered worldwide, of which 126 are known to occur in Hungary. Thanks to the last decade’s efforts in the Hungarian myrmecological research, and because of the constantly changing taxonomy of several problematic ant genera, a new checklist of the Hungarian ants is presented here. The state of the Hungarian myrmecofauna is also discussed in the context of other European countries’ ant fauna. Six species (Formica lemani, Lasius nitidigaster, Tetramorium immigrans, T. staerckei, T. indocile and Temnothorax turcicus) have been reported for the first time in the Hungarian literature, nine taxon names were changed after systematic replacements, nomenclatorial act, or as a result of splitting formerly considered continuous populations into more taxa. Two species formerly believed to occur in Hungary are now excluded from the updated list. All names are nomenclaturally assessed, and complete synonymies applied in the Hungarian literature for a certain taxon are provided. Wherever it is not self-evident, comments are added, especially to explain replacements of taxon names. Finally, we present a brief descriptive comparison of the Hungarian myrmecofauna with the ant fauna of the surrounding countries. The current dataset is a result of ongoing work on inventorying the Hungarian ant fauna, therefore it is expected to change over time and will be updated once the ongoing taxonomic projects are completed.
Investigating the Effect of Interface Morphology in Adhesively Bonded Composite Wavy-Lap Joints
Authors: Akrami Roya , Anjum Shahwaiz , Fotouhi Sakineh , Boaretto Joel , de Camargo Felipe Vannucchi , Fotouhi Mohamad
Published: January 2021

Joints and interfaces are one of the key aspects of the design and production of composite structures. This paper investigates the effect of adhesive–adherend interface morphology on the mechanical behavior of wavy-lap joints with the aim to improve the mechanical performance. Intentional deviation from a flat joint plane was introduced in different bond angles (0°, 60°, 90° and 120°) and the joints were subjected to a quasi-static tensile load. Comparisons were made regarding the mechanical behavior of the conventional flat joint and the wavy joints. The visible failure modes that occurred within each of the joint configurations was also highlighted and explained. Load vs. displacement graphs were produced and compared, as well as the failure modes discussed both visually and qualitatively. It was observed that distinct interface morphologies result in variation in the load–displacement curve and damage types. The wavy-lap joints experience a considerably higher displacement due to the additional bending in the joint area, and the initial damage starts occurring at a higher displacement. However, the load level had its maximum value for the single-lap joints. Our findings provide insight for the development of different interface morphology angle variation to optimize the joints behavior, which is widely observed in some biological systems to improve their performance.
Novel α + β Zr Alloys with Enhanced Strength
Authors: Veverková Anna , Preisler Dalibor , Zimina Mariia , Košutová Tereza , Harcuba Petr , Janeček Miloš , Stráský Josef
Published: January 2021

Low-alloyed zirconium alloys are widely used in nuclear applications due to their low neutron absorption cross-section. These alloys, however, suffer from limited strength. Well-established guidelines for the development of Ti alloys were applied to design new two-phase ternary Zr alloys with improved mechanical properties. Zr-4Sn-4Nb and Zr-8Sn-4Nb alloys have been manufactured by vacuum arc melting, thermo-mechanically processed by annealing, forging, and aging to various microstructural conditions and thoroughly characterized. Detailed Scanning electron microscopy (SEM) analysis showed that the microstructural response of the alloys is rather similar to alpha + beta Ti alloys. Duplex microstructure containing primary alpha phase particles surrounded by lamellar alpha + beta microstructure can be achieved by thermal processing. Mechanical properties strongly depend on the previous treatment. Ultimate tensile strength exceeding 700 MPa was achieved exceeding the strength of commercial Zr alloys for nuclear applications by more than 50%. Such an improvement in strength more than compensates for the increased neutron absorption cross-section. This study aims to exploit the potential of alpha + beta Zr alloys for nuclear applications.
Time- and Temperature-Dependent Luminescence of Manganese Ions in Ceramic Magnesium Aluminum Spinels
Authors: Khaidukov Nicholas , Pirri Angela , Brekhovskikh Maria , Toci Guido , Vannini Matteo , Patrizi Barbara , Makhov Vladimir
Published: January 2021

Samples of magnesium aluminum spinel ceramics doped with manganese ions were prepared by a high-temperature solid-state reaction method; their potential as red-emitting phosphors was analyzed using a time-resolved luminescence spectroscopy technique, from room temperature to 10 K. It was found that in the red spectral range, the luminescence spectra of manganese ions in the MgAl2O4 spinel showed a narrow band peaking at 651 nm due to the emission of Mn4+ and a broader emission band in the region of 675 ÷ 720 nm; the ratio of intensities for these bands depends on the synthesis conditions. By applying a special multi-step annealing procedure, the MgAl2O4:Mn4+ phosphor containing only tetravalent manganese ions, Mn4+, was synthesized. Broad-band far-red emission observed from MgAl2O4:Mn and Mg1.25Al1.75O3.75F0.25:Mn phosphors, prepared by a conventional method of a solid-state reaction, was interpreted as coming from Mn3+ ions.
Case Study of the Effect of Precoating on the Decarburization of the Surface Layer of Forged Parts during the Hot Die Forging Process
Authors: Widomski Paweł , Zwierzchowski Maciej , Barełkowski Artur , Tympalski Mateusz
Published: January 2021

This paper aims to evaluate the effect of pre-coating of forged parts on decarburization in the die forging process. The studies consisted of three stages. In the first instance, different coatings were tested under laboratory conditions by heating steel samples to the temperature of 1200 °C for over five minutes to model the preheating conditions of the induction. Next, testing continued in a commercial forging stand where we tested the effects of different coatings on the rods decarburization during the induction heating process, usually performed before forging. Once completed testing, the measurements and observations of the decarbonized layer were made. The third stage involved analysis of the decarburization of the forged parts after forging. The forged parts were made using precoating of pre-forging elements; pieces cut off a metal rod. Based on tests results, the possibility of using this solution in the technique of industrial hot forging was evaluated. The results of laboratory tests have confirmed that lubrication of metal pieces is sufficient, as well as proved it to be effective in reducing decarburization of the surface layer. Research works conducted in an induction heater showed differences in decarburization depending on a substance and concentration of lubricants that were used. These differences become more apparent when observing the surface layer of the forged parts. Results indicate that decarburization may be reduced to a minimum when we use Bonderite product in a concentration of 66% and 50%. Another lubricant, Berulit 913, may also be used. However, due to burning graphite in high temperatures, reduction of decarburization goes only as far as half of the thickness of the decarbonized layer. Condursal has no significant effect; nevertheless, it protects over the induction heating stage.
A Numerical Study of Geometry’s Impact on the Thermal and Mechanical Properties of Periodic Surface Structures
Authors: Gawronska Elzbieta , Dyja Robert
Published: January 2021

The paper focuses on thermal and mechanical analysis of Periodic Surface Structure (PSS). PSS is a continuous surface with a specific topology that is mathematically formulated by geometric factors. Cubic P-surface (“primitive”), D-surface (“diamond”), and G-surface (“gyroid”) structures were simulated under load and heat transport using a numerical approach. We conducted our study by solving the stress and heat equations using the Finite Element Method (FEM). We achieved results using our software module, which generates PSS and simulates stress and temperature distribution. The stress model defined by dependence between stress and strain, gained from an experiment, and correlation of strain and displacement, gained from geometric conditions, was used in numerical experiments. The influence of geometric factors on the thermal and mechanical behavior of PSS was qualitatively determined. We showed decreasing effective stress values with an increased number of cells in the cubic domain for concerned PSS. It is important, because the increase in the number of cells does not increase the structure’s volume.
Interior Sound Field Subjective Evaluation Based on the 3D Distribution of Sound Quality Objective Parameters and Sound Source Localization
Authors: Jin Jiangming , Cheng Hao , Xie Tianwei , Lu Huancai
Published: January 2021

Controlling low frequency noise in an interior sound field is always a challenge in engineering, because it is hard to accurately localize the sound source. Spherical acoustic holography can reconstruct the 3D distributions of acoustic quantities in the interior sound field, and identify low-frequency sound sources, but the ultimate goal of controlling the interior noise is to improve the sound quality in the interior sound field. It is essential to know the contributions of sound sources to the sound quality objective parameters. This paper presents the mapping methodology from sound pressure to sound quality objective parameters, where sound quality objective parameters are calculated from sound pressure at each specific point. The 3D distributions of the loudness and sharpness are obtained by calculating each point in the entire interior sound field. The reconstruction errors of those quantities varying with reconstruction distance, sound frequency, and intersection angle are analyzed in numerical simulation for one- and two-monopole source sound fields. Verification experiments have been conducted in an anechoic chamber. Simulation and experimental results demonstrate that the sound source localization results based on 3D distributions of sound quality objective parameters are different from those based on sound pressure.
Characterization of Properties, In Vitro and In Vivo Evaluation of Calcium Phosphate/Amino Acid Cements for Treatment of Osteochondral Defects
Authors: Medvecky Lubomir , Giretova Maria , Stulajterova Radoslava , Danko Jan , Vdoviakova Katarina , Kresakova Lenka , Zert Zdenek , Petrovova Eva , Holovska Katarina , Varga Maros , Luptakova Lenka , Sopcak Tibor
Published: January 2021

Novel calcium phosphate cements containing a mixture of four amino acids, glycine, proline, hydroxyproline and either lysine or arginine (CAL, CAK) were characterized and used for treatment of artificial osteochondral defects in knee. It was hypothesized that an enhanced concentration of extracellular collagen amino acids (in complex mixture), in connection with bone cement in defect sites, would support the healing of osteochondral defects with successful formation of hyaline cartilage and subchondral bone. Calcium phosphate cement mixtures were prepared by in situ reaction in a planetary ball mill at aseptic conditions and characterized. It was verified that about 30–60% of amino acids remained adsorbed on hydroxyapatite particles in cements and the addition of amino acids caused around 60% reduction in compressive strength and refinement of hydroxyapatite particles in their microstructure. The significant over-expression of osteogenic genes after the culture of osteoblasts was demonstrated in the cement extracts containing lysine and compared with other cements. The cement pastes were inserted into artificial osteochondral defects in the medial femoral condyle of pigs and, after 3 months post-surgery, tissues were analyzed macroscopically, histologically, immunohistochemically using MRI and X-ray methods. Analysis clearly showed the excellent healing process of artificial osteochondral defects in pigs after treatment with CAL and CAK cements without any inflammation, as well as formation of subchondral bone and hyaline cartilage morphologically and structurally identical to the original tissues. Good integration of the hyaline neocartilage with the surrounding tissue, as well as perfect interconnection between the neocartilage and new subchondral bone tissue, was demonstrated. Tissues were stable after 12 months’ healing.
Laser Metal Deposition of Inconel 718 Alloy and As-built Mechanical Properties Compared to Casting
Authors: Mazzucato Federico , Forni Daniele , Valente Anna , Cadoni Ezio
Published: January 2021

In the last years, powder-based Laser Metal Deposition (LMD) has been attracting attention as a disruptive Additive Manufacturing (AM) technique for both the fabrication and restoration of Inconel 718 components, enabling to overcome current limitations faced by conventional manufacturing processes in terms of manufacturing costs, tool wear, and lead time. Nevertheless, the uncertainty related to the final mechanical performance of the as-built LMD parts limits a wider adoption of such technology at industrial level. This research work focuses on the mechanical characterization of as-built Inconel 718 specimens through split Hopkinson tensile bar tests performed at different strain rate conditions. The influence of laser power on the final mechanical behavior of the as-built tensile samples is discussed and compared with the mechanical response of as-cast ones. The as-built specimens exhibit a high internal density (i.e., 99.92% and 99.90% for 300 W and 400 W, respectively) and a more ductile behavior compared to the as-cast ones for every evaluated strain rate condition. The strain hardening capacity of the as-built samples increases with the laser power involved in the LMD process, reaching an average Yield Strength of 703 MPa for specimens realized at 400 W and tested at 800/s.
Microwave Sintering and Microwave Dielectric Properties of (1–x)Ca0.61La0.26TiO3-xNd(Mg0.5Ti0.5)O3 Ceramics
Authors: Yang Shuwei , Liang Bingliang , Liu Changhong , Liu Jin , Fang Caisheng , Ai Yunlong
Published: January 2021

The (1–x)Ca0.61La0.26TiO3-xNd(Mg0.5Ti0.5)O3 [(1–x)CLT-xNMT, x = 0.35~0.60] ceramics were prepared via microwave sintering. The effects of sintering temperature and composition on the phase formation, microstructure, and microwave dielectric properties were investigated. The results show that the microwave sintering process requires a lower sintering temperature and shorter sintering time of (1–x)CLT-xNMT ceramics than conventional heating methods. All of the (1–x)CLT-xNMT ceramics possess a single perovskite structure. With the increase of x, the dielectric constant (ε) shows a downward trend; the quality factor (Qf) drops first and then rises significantly; the resonance frequency temperature coefficient (τf) keeps decreasing. With excellent microwave dielectric properties (ε = 51.3, Qf = 13,852 GHz, τf = −1.9 × 10−6/°C), the 0.65CLT-0.35NMT ceramic can be applied to the field of mobile communications.
In Vitro Evaluation of Antioxidant Potential of the Invasive Seagrass Halophila stipulacea
Authors: Sansone Clementina , Galasso Christian , Lo Martire Marco , Fernández Tomás Vega , Musco Luigi , Dell’Anno Antonio , Bruno Antonino , Noonan Douglas M. , Albini Adriana , Brunet Christophe
Published: January 2021

Marine organisms with fast growth rates and great biological adaptive capacity might have biotechnological interests, since ecological competitiveness might rely on enhanced physiological or biochemical processes’ capability promoting protection, defense, or repair intracellular damages. The invasive seagrass Halophila stipulacea, a non-indigenous species widespread in the Mediterranean Sea, belongs to this category. This is the premise to investigate the biotechnological interest of this species. In this study, we investigated the antioxidant activity in vitro, both in scavenging reactive oxygen species and in repairing damages from oxidative stress on the fibroblast human cell line WI-38. Together with the biochemical analysis, the antioxidant activity was characterized by the study of the expression of oxidative stress gene in WI-38 cells in presence or absence of the H. stipulacea extract. Concomitantly, the pigment pool of the extracts, as well as their macromolecular composition was characterized. This study was done separately on mature and young leaves. Results indicated that mature leaves exerted a great activity in scavenging reactive oxygen species and repairing damages from oxidative stress in the WI-38 cell line. This activity was paralleled to an enhanced carotenoids content in the mature leaf extracts and a higher carbohydrate contribution to organic matter. Our results suggest a potential of the old leaves of H. stipulacea as oxidative stress damage protecting or repair agents in fibroblast cell lines. This study paves the way to transmute the invasive H. stipulacea environmental threat in goods for human health.
In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
Authors: Krasowski Grzegorz , Junka Adam , Paleczny Justyna , Czajkowska Joanna , Makomaska-Szaroszyk Elżbieta , Chodaczek Grzegorz , Majkowski Michał , Migdał Paweł , Fijałkowski Karol , Kowalska-Krochmal Beata , Bartoszewicz Marzenna
Published: January 2021

Chronic wounds complicated with biofilm formed by pathogens remain one of the most significant challenges of contemporary medicine. The application of topical antiseptic solutions against wound biofilm has been gaining increasing interest among clinical practitioners and scientific researchers. This paper compares the activity of polyhexanide-, octenidine- and hypochlorite/hypochlorous acid-based antiseptics against biofilm formed by clinical strains of Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa. The analyses included both standard techniques utilizing polystyrene plates and self-designed biocellulose-based models in which a biofilm formed by pathogens was formed on an elastic, fibrinous surface covered with a fibroblast layer. The obtained results show high antibiofilm activity of polihexanide- and octenidine-based antiseptics and lack or weak antibiofilm activity of hypochlorite-based antiseptic of total chlorine content equal to 80 parts per million. The data presented in this paper indicate that polihexanide- or octenidine-based antiseptics are highly useful in the treatment of biofilm, while hypochlorite-based antiseptics with low chlorine content may be applied for wound rinsing but not when specific antibiofilm activity is required.
Simultaneous Measurement of Amino Acid Enantiomers in Aged Mouse Brain Samples by LC/MS/MS Combined with Derivatization Using Nα-(5-Fluoro-2,4-dinitrophenyl)-l-leucinamide (l-FDLA)
Authors: Yamamoto Taiji , Yaku Keisuke , Nakagawa Takashi
Published: January 2021

d-amino acids have distinct roles from their l-enantiomer. In particular, some d-amino acids function as agonists or antagonists of neuronal receptors and are involved in higher brain functions. Thus, it is important to precisely measure the levels of these amino acid enantiomers in cells and tissues. Various quantification methods have been developed for measurements of chiral amino acids. However, each method has advantages and disadvantages. Additionally, measuring the amino acid enantiomers in crude biological samples requires a higher selectivity. In this study, we developed a quantification method for amino acid enantiomers using derivatization with Nα-(5-Fluoro-2,4-dinitrophenyl)-l-leucinamide (l-FDLA) followed by liquid chromatography–tandem mass spectrometry (LC/MS/MS) with a conventional reversed-phase column. We simultaneously identified 10 chiral amino acids. Furthermore, we applied this method to investigate murine tissue samples and examined the effect of aging on the amino acid levels in aged brain regions. We found that aging decreased the levels of both d-serine and d-aspartate in the hippocampus. In addition, d-Phenylalanine in the thalamus significantly increased with age. In conclusion, our method is suitable for the quantification of the d-amino acids in crude biological samples and may contribute to elucidating the biological roles of chiral amino acids.
Hair Loss During Menopause: Ways of the Problem Solution
Authors:   Russian Medical Academy of Continuous Professional Education, Gallyamova Yu.A. ,   ‘X. About The Lab’, LLC, Korableva O.A. , Doskina Ye.V. , Khodova O.O.
Published: January 2021

Provided data on the effectiveness of a new peptide complex in the treatment of female-type hair loss in postmenopausal patients. Thus, the use of the complex contributed to the transition of follicles from the resting stage to the growth stage. As a result, the hair density increased