bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronicle of getting the diagnostic laboratory NABL accredited in a teaching medical institute: An overview of our experience
Authors: Shetty Archana , Minal Jessica , H D Shilpa
Published: January 2024

Introduction and Aim: Accreditation of medical laboratories by National Accreditation Board for Testing and Calibration Laboratories (NABL) ISO 15189 is needed to improve quality and safety in healthcare. It is a rigorous third-party external evaluation process that encompasses assessment against a given set of standards. The aim is to narrate the process of accreditation of a diagnostic laboratory affiliated to a newly established teaching medical institute and elaborate various facets which were worked upon from initialization till the culmination of the assessment.
 
Methods: Bulk of data is the author’s first-hand experience as all worked hands-on in getting the NABL accreditation from July 2022 to January 2023. For standards and technical information guidelines of NABL & ISO 15189:2012 was referred. Input from peers working in accredited labs was taken. Entire process of preparation and applying for accreditation aspects was compiled and presented.
 
Results: With interdepartmental coordination and changes in workflow process complemented by document control procedures culminated in the laboratory successfully getting accredited.
 
Conclusion: The entire process of getting accreditation was challenging but nevertheless gratifying. For medical laboratories affiliated to teaching institutes accreditation needs extra time and effort but is nonetheless achievable.
Colistin and Polymyxin B sensitivity by broth microdilution among carbapenem-resistant clinical isolates of Gram-negative bacilli
Authors: Bernhardt Jyothsna Sheethal , Tellis Rouchelle Charmaine , Motagi Aravind , P. P. Asif
Published: January 2024

Introduction and Aim: The increasing incidence of multidrug-resistant gram-negative bacilli (MDR-GNB) in recent times has led to the revival of older neglected antibiotics such as Polymyxins. Hence, there is a need to determine the minimum inhibitory concentration (MIC) of these antibiotics by conventional and automated antibiotic sensitivity testing methods to assess their clinical utility. This study aimed to assess the in vitro sensitivity of clinical isolates grown with carbapenem-resistant GNB (CR-GNB) to Colistin and Polymyxin B by broth micro-dilution method (BMD).
 
Materials and Methods: Thirty isolates of CR-GNB obtained from various clinical samples received in the microbiology laboratory of Yenepoya Medical College Hospital between August 2018 to November 2019 were included in the study. In vitro assessment of Colistin and Polymyxin B sensitivity among CR-GNB was performed by BMD as per CLSI guidelines.
 
Results: Twelve isolates (40%) were carbapenemase producers. Among the 30 isolates of CR-GNB, 56.6% and 63.3 % of them were sensitive to Colistin and Polymyxin, respectively. Colistin MICs of 12 isolates obtained by BMD assay were concordant with the MICs obtained by BD Phoenix.
 
Conclusion: The results of this study indicate that Colistin and Polymyxin B can be considered as reserve therapeutic agents for treating infections caused by CR-GNB after obtaining the in vitro sensitivity results.
Association of bone turnover markers with glucose metabolism in type 2 diabetes mellitus
Authors: Rai Srinidhi , Rai Tirthal , D'Sa Janice
Published: January 2024

Introduction and Aim: Type 2 diabetes mellitus (Type 2 DM) subjects are at increased risk of fracture due to poor bone quality contributing to diabetic skeletal fragility. This study provides an understanding of the relationship between bone turnover markers (BTM) and glucose metabolism in Type 2 DM.
 
Materials and Methods: One hundred types 2 diabetics and one hundred controls participated in this case control study. Serum FBS, osteocalcin, alkaline phosphatase (ALP), CTx (C- terminal cross linked telopeptide of type 1 collagen), HOMA-IR and HOMA-B was measured. Association between BTM and FBS, HOMA-IR and HOMA-B was studied. Comparison was done by Student’s t test. The correlation was established using Pearson's correlation test. P value less than 0.05 was used to determine statistical significance.
 
Results: Mean levels of FBS, ALP and HOMA-IR were higher and mean levels of osteocalcin, CTx and HOMA-B were decreased in diabetics when compared to controls.  Osteocalcin levels showed significant negative correlation with FBS, ALP, HOMA-B and HOMA-IR. CTx levels were negatively correlated with ALP and FBS.
 
Conclusion: Early identification of decrease in bone mass in diabetic patients may be helpful in preventing bone loss and future fracture risks. Monitoring for diabetes associated with osteopenia is essential. The biochemical markers of bone metabolism can detect even small changes in the bone turnover, thus identifying diabetics who may become osteoporosis-prone.
Evaluating microRNA-499 expression and serum IL-17, ACCP and PADI4 cytokine levels in rheumatoid arthritis patients undergoing anti-TNF alpha therapy
Authors: Jumaah Fadhil Zainab , Abdul-Hassan Abbas Ahmed , Hadi Al-Osami Mohammad
Published: January 2024

Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by joint inflammation and damage. While anti-TNF-alpha therapies like infliximab have revolutionized RA, treatment response varies among patients. Identifying reliable biomarkers for treatment response is crucial for optimizing therapy and patient outcomes. MicroRNA-499 has been implicated in autoimmune diseases, and IL-17 is a key mediator in RA pathogenesis. Additionally, PADI4 and ACCP are linked to RA autoimmunity. This study aimed to investigate the potential of microRNA-499 expression, serum levels of IL-17, PADI4 and ACCP as biomarkers for predicting and monitoring treatment response in RA patients undergoing anti-TNF-alpha (infliximab) therapy.
 
Methodology: In this case-control research, blood samples from 100 Iraqi patients with RA and 100 controls were collected. RNA was extracted and the levels of miRNA499 were quantified using real-time PCR. The serum IL-17, PADI4 and ACCP levels were determined using ELISA. Patients were sub-grouped as responders and non-responders based on their responses to anti-TNF-alpha (infliximab) treatment.
 
Results: The mean age of the patients in this study was 46.22 years. The occurrence of RA was higher in age group (31-45) years. There was a significant association between RA, smoking and BMI. miRNA-499 expression was significantly higher in RA patients versus controls (1.1 folds in patients compared to 0.52 in control group). The serum levels of IL-17, PADI4 and ACCP was significantly higher in RA patients than controls (54.3ng/L, 2.52 ng/ml and 2.15 U/ml) vs (40.35ng/ L, 1.78 ng/ml and 1.06 U/ml) respectively. Subdividing of patients showed that miRNA-499 expression and serum IL-17, PADI4 and ACCP to be significantly higher in non-responders patients than responders.
 
Conclusion: MicroRNA-499 expression, serum levels of IL-17, PADI4 and ACCP were found to be significantly higher in RA patients compared to control group as well as in non-responding than responding patients.
Anatomical and histological assessment of Arabic gum administration on visceral organs of diabetic rats
Authors: H. Majeed Alnassiri Shurooq , S. Rashid Muna , A. Hameed Huda
Published: January 2024

Introduction and Aim: This century has seen a rise in the prevalence of diabetes mellitus, and since medications pose a number of risks and adverse effects, researchers have been experimenting with a variety of natural materials, including herbs. One among the herbs is Arabic gum which is considered beneficial in treating diabetes. In this study, we investigated the anatomical and histological effect of Arabic gum treatment in diabetes-induced rats when administered at different concentrations.
 
Materials and Methods In this study, albino rats were divided into four experimental groups: control, alloxan administered, alloxan and Arabic gum (5%) administered, and alloxan and Arabic gum (10%) administered. Following the experimental period, the four groups of animals were sacrificed and their anatomical and histopathological changes were studied.
 
Results: This study showed that alloxan induced diabetes caused liver, kidney and lung injuries. The groups that received alloxan with Arabic gum at concentrations 5 % and 10% exacerbated the organ damage. Several anatomical changes of the visceral organs were seen in relation to animals in the control group. Morphologically, groups that received Alloxan and Arabic gum had white spot lesions on the surfaces of the liver kidney and lungs. Histopathological changes were also observed in the liver, kidney and lungs of the animals when compared to controls.  
 
Conclusion: Organs such as the liver, kidneys, and lungs were found to be damaged in alloxan-induced diabetic rats. The administration of Arabic gum at concentrations of 5% and 10% does not stop the damage to these organs.
Evans syndrome: A case report
Authors: Hegde Megha , Raj Saurav , Ashvil Anil , Tikadar Dhananjay , Bharamu Nyamagoud Sanatkumar
Published: January 2024

Evans syndrome is described as the simultaneous presence of immune thrombocytopenia, warm autoimmune haemolytic anaemia, and sometimes autoimmune neutropenia. It occurs due to autoantibodies that fail to cross-react with antigens unique to platelets, red blood cells, or neutrophils. Haemolysis and thrombocytopenia may be causing clinical symptoms. Evans syndrome stands as a rare condition diagnosed through a process of exclusion. Initial treatment options encompass intravenous corticosteroids or intravenous immunoglobulins, serving as the first choice of interventions. In cases where patients do not respond to steroids, the subsequent steps involve second-line treatments such as rituximab or splenectomy. A 43-year-old female with a history of Evans syndrome presented with a complaint of generalised weakness for 20 days after having recently been tapered off of prednisone. She was treated with a combination of immunomodulators and corticosteroids. Here, we try to highlight the importance of medication adherence which is essential in treating chronic disorders.
Comparative assessment of edible oil oxidative stability through accelerated stability study
Authors: Selvi Moorthy Karthika , N. Nagalapura Aishwarya , P. Vijayaraj
Published: January 2024

Introduction and Aim: The oxidative stability of edible oils is a critical factor influencing their health benefits and suitability for various food applications. The fatty acid composition significantly impacts the susceptibility of oils to oxidation. Edible oils play a pivotal role in cooking and food production, making their oxidative stability a matter of paramount importance. Oxidative stability affects product quality, shelf life, nutritional value, and health benefits. Accelerated oxidative stability tests have emerged to address these challenges more efficiently. Therefore, the objective of the current study was to evaluate the oxidative stability of five edible oils at consistent 90°C: Fish Oil (FO), Coconut Oil (CO), Rice Bran Oil (RBO), Sunflower Oil (SFO) and Palm Oil (PO).
 
Materials and Methods: Edible oils were procured from the local market.  Fish Oil (FO) was acquired from Janatha Aqua products, the oxidative stability of oils was evaluated by using OXITEST apparatus.
 
Results: The observed results revealed distinct oxidative stability profiles for each oil. CO exhibited the highest stability with an Oxidation Induction Time (OIT) of 136 hours and 38 minutes, highlighting its suitability for high-temperature cooking. PO and RBO showed moderate stability (OIT: 49h 11m and 26h 15m). SFO displayed lower stability (OIT: 10h 9m), while FO was the least stable (OIT: 54m) due to its high unsaturated fatty acid content.
 
Conclusion: Collectively, the OXITEST provides valuable insights into the oxidative stability of fats and oils, standing out for its real-time, accelerated testing capabilities, and providing faster insights into oxidation behaviour compared to traditional methods.
Correlation between serum cortisol levels and practice of yoga and meditation in medical students
Authors: M.M. Kavitha , S. H. Chandrashekharayya , V.S. Anagha , S.V. Kashinakunti
Published: January 2024

Introduction and Aim: Recent year’s yoga has gained a very good response in today’s world as one of the natural methods to reduce stress. Yoga acts as an adjuvant to many kinds of treatment for various stress-related disorders. To meet the modern lifestyle full of tension, stress and challenges and to gain all round personality development yoga is mandatory. With this background current study was carried out, to estimate and compare the serum cortisol levels and heart rate variability and also to evaluate the relationship between serum cortisol levels, heart rate variability before and after yoga and meditation practice for six weeks in study group and control group in first year medical students.
 
Material and Methods: Informed consent was obtained before the start of the study. The current study was a quasi-experimental study with 80 subjects, 40 medical students as cases which is an interventional group and 40 students as a control group. For a period of six weeks, yoga and meditation practice was given to an interventional group by a yoga master daily for one hour.
 
Results: After practicing yoga regularly and promptly, cases showed decrease in serum cortisol levels and improvement in HRV parameters, frequency domain values like RMSSD increased and among time domain values HF increased and LF, LF/HF ratio decreased. There was no change observed in serum cortisol levels and HRV parameters of the control group. We observed statistically significant negative correlation between serum cortisol levels and HRV parameters in the interventional group after yoga practice.  
 
Conclusion: Hence, incorporation of yoga and meditation as part of our lifestyle in medical students will help them to cope up with day-to-day stress during their study period.
Recent advances in oral cancer - A review
Authors: Mallya P. Sachidananda , B. AppalaRaju , Mallya Shrikara
Published: January 2024

Carcinoma of oral cavity presents as a long-lasting sore in the mouth cavity. In the US more than 55000 cases of mouth cancer are recorded every year out of which around 70% were males. It can be deadly if not investigated and treated promptly. Oral carcinoma is a deadly health affair in India. WHO has reported that around one third of cases of oral carcinoma  in the world are found in India . ICMR had  announced  more than 75000 cases of oral carcinoma in India in 2020 . Mouth cancer can involve  any portion of the mouth, such as  the tongue, lips , gums, roof and bottom of the mouth, and the inner part of the cheeks, soft palate, hard palate sinuses, and pharynx . It occurs when cells in the mouth grow abnormally and form a malignant tumor. Tumors can also occur in the salivary glands, tonsils but these are less common. Mouth carcinoma can also disseminate to other portions of the body if proper treatment was not given. Oral cancer is significantly easier to treat when it is detected early. However, most people are diagnosed when their ailment is too advanced to be adequately treated. If one visits the dentist or doctor on a regular basis and learns how to recognize abnormal changes, it is possible to do an early diagnosis.
Effect of education on knowledge, attitude and practices of safety precautions among laboratory technicians of a tertiary care center in Mangalore
Authors: B. V. Mamatha , M.Bhat Bhagyajyothi , Shreya Crasta Preeval , P. Khadilkar Niranjan
Published: January 2024

Introduction and Aim: The clinical laboratory technicians are expected to have knowledge, to have a favourable attitude and practice safety precautions at their workplace. Though they are trained with safety precautions during their study period, they may not adhere to them upon joining the job. The present study was aimed at assessing the effect of educational intervention on knowledge, attitude and practices of safety precautions among laboratory technicians of our medical college hospital.
 
Materials and Methods: This interventional study included all the technicians. A validated questionnaire containing questions related to personal information, knowledge, attitude and practices of safety precautions was used. Pre-test was conducted followed by a lecture session regarding safety precautions.  After one hour, post-test was conducted in a similar way as pre-test and responses were collected. Data was analysed using the statistical software SPSS version 28. Qualitative variables were presented as frequency and percentage and quantitative variables as mean SD. McNemar test was employed to find the association between the knowledge, attitude questions with pre-test and post-test scores. Paired t test was used for comparison of knowledge and attitude overall scores. p value less than 0.05 was considered statistically significant.
 
Results: The study showed a significant difference at the level of knowledge towards safety precautions before and after the educational intervention. There was no significant difference at the levels of attitude among the participants.
 
Conclusion: Regular training of safety precautions may improve the knowledge of laboratory technicians, which will benefit both the technicians and the institution.
Detection of the effect of synthetic siRNA on efflux pump MexA gene expression and antibiotic resistance in clinical isolates of Pseudomonas aeruginosa
Authors: M. Al-Qurashi Farah , Abbas Al-Draghi Wathiq
Published: January 2024

Introduction and Aim: Pseudomonas aeruginosa is a nosocomial infection with an ability to develop high levels of antibiotic resistance. The efflux pump system is one of the mechanisms that is linked to multidrug resistance in P. aeruginosa. In this study, we employed siRNA loaded on gold nanoparticles against the MexA efflux pump gene to decrease the MexA gene expression in P. aeruginosa and estimated antibiotic resistance after gene silencing.
 
Materials and Methods: This study examined four strains of P. aeruginosa isolated from patients in various hospitals in Baghdad. Bacteria isolated were identified by biochemical tests and Vitek compact 2 system.  Single-stranded siRNA (33bp) designed in this study was loaded onto gold nanoparticles (AuNPls). Detection of the MexA gene was carried out by conventional PCR technique. The expression of MexA gene was examined by qRT-PCR in order to determine if the siRNA have impacted on MexA gene expression and on the antibiotic resistance in

aeruginosa

 
Results: This study showed that the mRNA expression level of the MexA gene exhibited a decrease in fold change CT -2   in P. aeruginosa (isolates numbers 67, 66,49, and PDR(5p)) when examined in vitro. The specific fold change values observed were (0.202, 0.040, 0.063, and 0.163) respectively. The resistance percentages of antibiotics tested was observed to increase after MexA gene silencing.
 
Conclusion: Targeting the MexA gene with synthetic siRNA may be a unique approach to diminish P. aeruginosa resistance to antibiotics. However, many unexpected consequences may occur when utilizing any genetic manipulation in bacteria.
Safety and efficacy of a combined treatment with corneal collagen cross-linking and lamellar keratoplasty in patients with keratitis
Authors: Turgunbaev Nurlan , Umetaliev Yusupzhan , Gogaeva Laila , Ostroverkhov Aleksandr , Yrysov Keneshbek
Published: January 2024

Introduction and Aim: There is a link between an increase in eye infections, environmental deterioration, increased intensity of eye surgery, and the diversification of diagnostic techniques and the development of corneal disease and eye injuries. This study aims to assess the safety and efficacy of a complex treatment for corneal ulcers (keratitis) that combines corneal collagen cross-linking (CXL) with lamellar keratoplasty (LK).
 
Materials and Methods: This study consisted of 28 eyes from 28 patients with keratitis. All patients were divided into the main and control groups; each group had 14 patients (14 eyes). All patients underwent a standard ophthalmological examination, which included visometry without correction, with correction, tonometry, biomicroscopy, and ophthalmoscopy.
 
Results: High postoperative astigmatism was found in 50% of cases (7 eyes) of the control group and in 21.4% of cases (3 eyes) of the main group over the 1-6 months follow-up period. Both groups visual acuity development dynamics were very similar from the three-month to three-year follow-up period, and the average visual acuity with maximal correction was between 0.4 - 0.5.
 
Conclusion: Ultraviolet radiation exposure during CXL increases the graft's strength, the recipient's corneal tissue in the suture area, and the pathological flora's direct bactericidal and virucidal effects, reducing infectious process recurrences.
Increased circulatory plasma and urinary exosomal microRNA-191a as a prognostic biomarker in patients with nephrotic syndrome
Authors: Usman Bedrabettu Rahinaz , Pai B. H. Santhosh , Rao C. K. Anuradha
Published: January 2024

Introduction and Aim: Nephrotic syndrome is one of the most common problems in kidney disease. Renal biopsy is the only standard method that determines the renal cells scarring and which also classifies the pathology of the disease. It is one of the invasive procedures with its own potential complications. So generally, it will not be suitable for frequent monitoring. MicroRNAs (miRNAs), are the short noncoding RNA molecules that regulate gene expression and are conventionally constituted of 22–25 nucleotides. MicroRNAs by binding to messenger RNAs suppress the gene expressions and it regulates different path physiological processes. In renal tissue, miRNAs also regulate the tubular and glomerular damage and proteinuria. MicroRNA has the major role in various kidney diseases due to its functional role in apoptosis, cell proliferation, differentiation, and development Elevated levels of plasma and urinary microRNAs can predict a high risk of renal function loss, disease progression and histological diagnosis. miRNA plays a very significant role in disease monitoring as well. Thus, the miRNAs have potential which could be developed as biomarkers in diagnosed cases of nephrotic syndrome. The present study was conducted to find out the specific role of miRNA-191a in nephrotic syndrome.
 
Materials and Methods: Quantitative real-time PCR was used to detect the overexpressed plasma miRNA by Taqman microarray. U6sn RNA were selected as a control. Using a primer of miRNA191a, quantification of mi RNA-191a was done by RT-qPCR.
 
Results: We found significant difference in f mol concentration among nephrotic syndrome at diagnosis, nephrotic syndrome after follow-up of three months and in the control (one-way ANOVA F=1321).
 
Conclusion: Increased miRNAs in patients with nephrotic syndrome can be used as the potential biomarker for monitoring the diagnosed disease.
Exploring Mycobacterium tuberculosis microarray data: Identification of interleukin (IL) as a key deregulated gene family for targeted therapy with phytochemicals
Authors: Sayyed Shamabanu , A.S. Sriranjini , Selvakumar Kamalnath , L.S. Hallur Raghavendra
Published: January 2024

Introduction and Aim: The contemporary global health landscape is shadowed by the persistent menace of Mycobacterium tuberculosis, the etiological agent of tuberculosis (TB), a recalcitrant infectious malady. Despite medical advances, TB remains a formidable health threat, necessitating innovative strategies. Emerging drug-resistant strains further complicate its management, compelling the quest for novel biomarkers and therapeutic targets. In this context, biomarker identification assumes salience for enhanced TB management.

 

Methods: The Microarray technology, with its multifaceted gene expression analysis capability, gains prominence in this endeavor. Employing microarray datasets, this study identified perturbed gene families, discerning nuanced TB pathogenesis and drug resistance through network pharmacology. In-silico computational techniques including molecular docking, molecular dynamic simulation and pharmacokinetics were employed to attain a comprehensive understanding of pharmacological attributes of phytocompounds towards interleukins.

 

Results: Intriguingly, the representatives of the IL family were overrepresented and highly deregulated. To address TB's therapeutic conundrum, the study synthesizes microarray insights with phytochemical potential. Strikingly, IL-6 manifested as a key player, engaging in substantial protein-protein interactions within the network. Molecular docking unveiled Quercetin, Apigenin, Luteolin, Eupatilin, and Jaceidin as potent IL-6 interactors, with Quercetin showcasing notable binding affinity.

.

Conclusion: Quercetin, an anti-inflammatory agent, targeting IL-6 might mitigate detrimental immune responses and tissue damage, aiding TB management. Further exploration involves in vitro and in vivo validation of Quercetin-IL-6 interactions, assessing their impact on TB immune responses and pathogenesis. This study pioneers’ potential interventions, merging gene insights with phytochemical prowess, towards innovative TB management.
Usefulness of antiemetics in clinical cancer treatment: An understudied topic in pharmacy
Authors: Pillappan Ramkumar , Bhandary Roopa , Devang Nayana , Shantaram Manjula
Published: January 2024

The administration of cytotoxic substances in cancer therapy leads to profound nausea and vomiting, hence impeding the progress of cancer treatment. Cancer radiation therapy is also known to induce nausea and vomiting. An inconvenient symptom would impede therapy and diminish the overall quality of life, causing anxiety and hopelessness and rendering everyday activities challenging When used together with dexamethasone, 5-HT3 receptor antagonists are highly efficient in reducing acute vomiting caused by cisplatin and mildly emetogenic therapy. Further research is required to gain a deeper understanding of the occurrence of CINV (Chemotherapy induced nausea and vomiting) in relation to new chemotherapies, particularly those taken orally on a daily basis. Determining the best combinations and timing of their administration in situations where chemotherapy is given over multiple days is important and the findings will greatly benefit patients by reducing treatment dropout rates and facilitating adherence to their chemotherapy regimens.
SNP IFNG rs1861494 gene polymorphism in pediatric patients with gastroenteritis and its relation to co-infectious with Adenovirus and Cryptosporidium
Authors: Hashim Abbas Fatima , S. Ibrahim Maryam , Hammad Mohammed Al-Alwany Shakir , A. Fatal Ghassan , Hasan Mohammed Ali Saad , Fadhil Bounya Sara
Published: January 2024

Introduction and Aim: The most common cause of infantile gastroenteritis is enteric adenoviruses, while Cryptosporidiosis is caused by Cryptosporidium. The function of IFN-gamma (IFNG/IFN-y) in immunological responses, inflammation, and autoimmune disease is increasingly recognized. This study aimed to examine intestinal co-infections of Cryptosporidium and adenovirus in pediatrics and the role of IFNG rs1861494 polymorphism to gastroenteritis among Iraqi children.
Materials and Methods: This case control study included 75 pediatric patients with severe gastroenteritis, aged 3-120 months, with a mean age of 30.64 months. The apparently healthy control (AHC) in this study included 25 pediatric individuals with a mean age of 27.64 months. Total genomic DNA extracted from stool specimens were subjected to PCR detection of human adenovirus virus. SNP IFNG rs1861494 polymorphism was detected using the ARMS-PCR technique.
Results: The percentage of males to female ratio in this study was 54:46. The positive PCR result of HADV7 was 28% (14 of 50 cases) while the positive result of Cryptosporidium was 6% (6 of 75 cases).Genotyping studies showed the GG genotype increased at rate OR=2.67 as compared to AG and AA genotypes for the SNP IFNG  rs1861494.  The SNP IFNG rs1861494 results in gastroenteritis were highly correlated with HADV7 and Cryptosporidium (respectively: r = 0.968, P = 0.007 as well as r = 0.984, P = 0.008).
Conclusions: Adenovirus diarrhea in Iraqi pediatric patients is associated with SNP rs1861494 polymorphism and could possibly be used as a biomarker in assessing the susceptibility of Iraqi children to the virus.
Aspectos da geologia e mineralogia do pegmatito Alto do Patrimônio, província pegmatítica da Borborema
Authors: Lisboa Vinicius Anselmo Carvalho , Macedo Juana Cristina de Abreu , Pereira Agda Rayanna Santos , Noberto Letícia Verônica , Galdino Manoel Pereira
Published: January 2024

O Pegmatito Alto do Patrimônio (PAP) localiza-se ao norte do estado da Paraíba, na cidade de Pedra Lavrada. Este corpo pegmatítico é, atualmente, explorado para a extração de minerais industriais. Está inserido no contexto da Província Pegmatítica da Borborema, intrudindo os metassedimentos da Formação Seridó. Esta província é mundialmente conhecida desde a II Guerra Mundial por seus pegmatitos mineralizados principalmente em tântalo-nióbio, berílio, estanho, lítio e minerais-gemas. Assim, o presente trabalho tem foco no mapeamento geológico de detalhe do PAP. O corpo apresenta comprimento com cerca 120 m de extensão, com espessura máxima de 46m, está orientado segundo as direções N-S. Exibe um zoneamento mineral bem definido, a saber: i) zona de borda (zona I); ii) zona de muro (zona II); iii) zona intermediária (zona III) e iv) núcleo de quartzo (zona IV). As zonas I e II contêm a mesma mineralogia essencial, composta por quartzo, feldspatos e micas. A zona III também é composta por quartzo, feldspatos e micas; é nela onde está concentrada a maior parte dos minerais de interesse econômico (principalmente a tantalita-columbita e o berilo). Na porção mais interna do pegmatito, o núcleo de quartzo, composto por quartzo leitoso e rosáceo. Esse zoneamento mineral observado permite classificá-lo como um pegmatito do tipo heterogêneo. A identificação desta mineralogia permitiu categorizá-lo como um pegmatito da família LCT, da classe dos pegmatitos ricos em elementos raros (ELR).
ПРОЄКТНО-ОРІЄНТОВАНЕ УПРАВЛІННЯ ТА ЙОГО РОЛЬ У РОЗВИТКУ ФІЗИЧНОЇ КУЛЬТУРИ ТА СПОРТУ НА РЕГІОНАЛЬНОМУ РІВНІ
Authors: Бондарець Єлизавета Миколаївна
Published: January 2024

У статті обґрунтовується доцільність використання проєктно-орієнтованого підходу до управління розвитком фізичної культури і спорту на регіональному рівні, для чого висунуто гіпотезу, що підвищення ефективності та результативності сучасного публічного управління розвитком фізичної культури та спорту на регіональному рівні можливо на засадах імплементації до діяльності органів публічного управління методології управління проєктами, заснованої на стандартах ISO, PMBOK та P2M, з одночасним використанням концепції проєктно-орієнтованого управління (або управління на основі проєктів – Management by Рroject), яка передбачає здійснення публічноуправлінської діяльності з реалізації сукупності (портфелю) проєктів з послідовним (або паралельним) керівництвом ними у міру переходу від одного проєкту до іншого. Удосконалено понятійно-категоріальний апарат предметної сфери дослідження, а саме: дістало подальшого розвитку тлумачення поняття «методологія управління проєктами розвитку фізичної культури та спорту», сформульовано авторські дефініції понять «проектно-орієнтований підхід до управління у сфері фізичної культури та спорту», «проєкт розвитку фізичної культури та спорту на регіональному рівні», «програма розвитку фізичної культури та спорту на регіональному рівні», «портфель проєктів з розвитку фізичної культури та спорту на регіональному рівні», на базі чого обґрунтовано принципи успішної реалізації проєктно-орієнтованого підходу до управління розвитком фізичної культури й спорту на регіональному рівні, до яких, зокрема, віднесено забезпечення комплексного впорядкованого та програмованого переходу від ситуації «без проєкту» до бажаної ситуації; відповідність цілям стратегії регіонального розвитку, визначеним як пріоритетні в конкретному регіоні, а також іншим програмно-цільовим документам вищого рівня, зокрема галузевої спрямованості; орієнтація на результативність та ефективність; впливовість та орієнтація на перспективу; синергетичний ефект. Зроблено висновок, що використання проєктно-орієнтованого підходу до управління розвитком фізичної культури та спорту на регіональному рівні буде сприяти удосконаленню діяльності органів публічного управління, наслідками якої має бути отримання суспільної цінності та задоволення колективних потреб (як цільових груп, так і кожного охочого мешканця регіону) у створенні належних умов для занять фізичною культурою та спортом на рівні кращих вітчизняних та міжнародних практик.
ДОСВІД ПУБЛІЧНОГО УПРАВЛІННЯ НАДАННЯ МЕДИЧНИХ ПОСЛУГ: МІЖНАРОДНИЙ АСПЕКТ
Authors: Турчин Катерина Юріївна
Published: January 2024

У статті розглянуто міжнародний досвід публічного управління надання медичних послуг. З’ясовано, що у світі функціонує три основні моделі системи охорони здоров'я: приватна, страхова та державна. Відмічено, що приватна модель охорони здоров'я характеризується наданням медичних послуг за рахунок власних коштів громадян та приватного страхування. Підкреслено, що відповідно до цієї моделі публічне управління надання медичних послуг здійснюється в децентралізованому порядку, а держава задовольняє ринок і надає медичні послуги лише соціально незахищеним верствам населення. Відзначено, що страхова модель спрямована на регулювання страхування здоров'я і поєднує в собі систему державного регулювання та соціальних гарантій і відносини на ринку медичних послуг. Акцентовано увагу на тому, що публічне управління у страховій моделі медичного обслуговування має ознаки децентралізації, зважаючи на велику кількість суб’єктів страхового ринку. Стверджено, що державна модель системи охорони здоров'я передбачає значну участь державних органів влади у процесі функціонування медичної сфери. Зазначено, що характерними ознаками публічного управління надання медичних послуг при державній моделі є високий ступінь централізації, при цьому більша частина медичних послуг надається державними лікувально-профілактичними установами. Аргументовано, що система охорони здоров’я жодної країни не базується на розглянутих вище моделях в чистому вигляді і жодна модель не є універсальною. Підтверджено на основі аналізу багаторічного міжнародного досвіду, що для суспільства дуже ризикованим і найменш ефективним способом фінансування охорони здоров'я є пряма оплата лікування хворих людей, зважаючи на високу вартість медичних послуг. Підсумовано, що міжнародний досвід надання медичних послуг доводить раціональність змішаної форми охорони здоров’я, в основі якої лежить гармонійне поєднання обов’язкового медичного страхування та бюджетного фінансування медицини як додаткового джерела фінансування. Доведено, що в українських реаліях найдоцільнішим є запозичення міжнародного досвіду функціонування змішаної моделі охорони здоров’я та її фінансування.
ДЕРЖАВНА ПОЛІТИКА В СФЕРІ ПРИРОДНИХ РЕСУРСІВ В УКРАЇНІ: АНАЛІЗ ТА НАПРЯМКИ УДОСКОНАЛЕННЯ
Authors: Трегубенко Галина Петрівна , Гладченко Дар’я Олександрівна
Published: January 2024

У статті розглянуто теоретичні засади реалізації державної політики в сфері природних ресурсів в Україні; охарактеризовано основні моделі втілення державної політики в сфері природних ресурсів в Україні; визначено фактори впливу на становлення вітчизняної державної політики в сфері природних ресурсів; проаналізовано загальнодержавний та регіональний аспекти державної політики в сфері природних ресурсів в Україні; визначено стратегічні цілі та інструменти реалізації державної політики в сфері природних ресурсів в Україні; досліджено стан євроінтеграційних процесів в сфері природних ресурсів, запропоновано напрямки вдосконалення державної політики в сфері природних ресурсів в Україні. Метою дослідження є удосконалення державної політики у сфері природних ресурсів в Україні шляхом формування та актуалізації нормативно-правового забезпечення, втілення економічних засад природокористування. У результаті проведеного дослідження було встановлено, що важлива роль у процесі забезпечення екологічної безпеки України належить запровадженню «Концепції сталого розвитку України», яка передбачає запровадження рамкових вимог щодо запровадження відповідних інструментів, які слугують покращенню навколишнього середовища та умов життєдіяльності населення. Інструментальна доцільність запровадження такої концепції зумовлена низкою об’єктивних факторів, які характеризують сучасний екологічний стан в Україні. У контексті аналізу оптимізаційних механізмів забезпечення переходу України до умов сталого розвитку окрему увагу також варто приділити запровадженню стратегічно орієнтованої державної екологічної політики, спрямованої на створення та збереження сприятливих умов навколишнього природного середовища. Її оптимізаційними складовими має виступати: підвищення якості життя і поліпшення здоров’я населення, підтримання екологічної рівноваги на території відповідно до прийнятих міжнародних правових екологічних вимог, а також національних і локальних нормативно-правих актів.
EFEKTIVITAS KONSUMSI VIRGIN COCONUT OIL dan SUSU KEDELAI TERHADAP PENINGKATAN PRODUKSI ASI IBU NIFAS
Authors: Wahyuni Iin , Utaminingtyas Farida , Apreliasari Helmy , Xanda Adhesty Novita , Aditia Dita Selvia , Destri Yuhelva , Muri Putri Hilwati
Published: January 2024

Providing breast milk (ASI) to babies aged 0-1 years has very important meaning, especially regarding meeting the needs for nutrients and other substances that form the body's immunity against disease. Exclusive breastfeeding at the age of 0-6 months is considered very strategic, because at that age the baby's condition is still very unstable and susceptible to various diseases. The coverage of babies < 6 months old receiving exclusive breastfeeding in Lampung Province in 2022 is 75.37%, where this figure has reached the expected target of 70% (Lampung Province Health Profile, 2022). The aim of the research is to analyze the effect of giving soy milk and VCO (Virgin Coconut Oil) on increasing breast milk production for postpartum mothers. This research method is quasi-experimental with a one group pre and post test design approach. The results of the research are that there is a difference in the increase in breast milk production of postpartum mothers who consume soy milk and VCO (virgin coconut oil) at the Archa Medica clinic in South Tangerang City with a Sig value. (2-tailed)/ p= 0.004. Conclusion: VCO and soy milk are proven to increase breast milk production. Suggestions: Providing information and knowledge to postpartum mothers regarding the benefits of soy milk and VCO to utilize natural ingredients available in the community for consumption to facilitate breast milk
HUBUNGAN PEMENUHAN GIZI DAN TINGKAT KECEMASAN DENGAN PENGELUARAN ASI PADA IBU POST PARTUM DI KOTA PADANGSIDIMPUAN TAHUN 2023
Authors: Pebrianthy Lola , Saragih Sarli
Published: January 2024

Ibu menyusui memerlukan gizi yang cukup untuk kesehatan tubuh dan bayinya. Gangguan psikologi pada ibu menyebabkan berkurangnya pengeluaran ASI. Penelitian ini bertujuan Untuk mengetahui hubungan pemenuhan gizi dan tingkat kecemasan dengan pengeluaran ASI pada ibu post partum di Kota Padangsidimpuan Tahun 2023. Jenis penelitian ini menggunakan desain Crossecsional study. Populasi dalam penelitian ini adalah  ibu post partum yang memberikan ASI kepada bayinya yaitu sebanyak 30 orang di Kota Padangsidimpuan dengan jumlah sampel adalah 30 orang. Teknik pengambilan sampel adalah total sampling. Hasil penelitian diperoleh bahwa ada hubungan antara pemenuhan gizi (p value = 0,025) dan tingkat kecemasan (p value = 0,001) dengan pengeluaran ASI pada ibu post partum di Kota Padangsidimpuan tahun 2023. Kesimpulan diperoleh bahwa ada hubungan pemenuhan gizi dan tingkat kecemasan dengan pengeluaran ASI pada ibu post partum. Diharapkan kepada ibu agar menambah pengetahuan mengenai pemenuhan gizi pada ibu post partum agar ibu dapat menghasilkan ASI yang banyak serta menjaga kondisi psikolgi yang baik agar tidak terjadi stres yang berlebihan.
The Relationship between giving formula milk and the incidence of diarrhea in babies 0-6 months in the Work Area of Batangtoru Public Health Center in 2023
Authors: Mutia Fatma , Lubis Arisa Harfa Said , Harahap Irawati
Published: January 2024

ABSTRACT
Giving formula milk too early will also reduce breast milk consumption, and if it is too late it will cause the baby to be malnourished and feeding at an early age will result in the baby's digestive ability not being ready to accept additional food. The problem of giving formula milk is greatly influenced by the baby's health behavior such as diarrhea. The mother's knowledge about giving formula milk and having a good attitude in giving formula milk can determine the best development for her child. The aim of this research is to determine the relationship between breastfeeding mothers regarding giving formula milk to babies 0-6 months with the incidence of diarrhea in the Batangtoru Community Health Center Work Area in 2023. This type of research is quantitative with a cross sectional approach method. The population in this study were all mothers who had babies aged 0-6 months, totaling 49 mothers. Because the population is less than 50 people, the sampling technique uses a total sampling technique. Chi Square Test results obtained p=0.000 (<0.05). So the conclusion is that there is a relationship between giving formula milk and the incidence of diarrhea in babies 0-6 months in the Batangtoru Health Center Working Area. 21 people were given formula milk, 21 people had diarrhea. It is recommended that the results of this study can provide information to respondents regarding knowledge of giving formula milk to babies 0-6 months with the incidence of diarrhea.
Keywords: Formula feeding, incidence of diarrhea, babies 0-6 months
HUBUNGAN DUKUNGAN KELUARGA DENGAN KEPATUHAN SELF CARE ACTIVITY PADA PENDERITA DM DI PUSKESMAS GUNUNG TUA TAHUN 2023
Authors: Sagala Nanda Suryani , Daulay Nanda Masraini , Napitupulu Natar Fitri , Yasin Kombang Ali , Hairani Winda May
Published: January 2024

Dukungan keluarga merupakan hal penting dalam penatalaksanaan pencegahan diabetes melitus untuk meminimalkan terjadinya komplikasi yang muncul. Self care activity diabetes merupakan tindakan membantu mengendalikan gula darah yang dapat menghasilkan kondisi kesehatan yang lebih baik. Penelitian ini bertujuan untuk mengetahui hubungan tingkat pengetahuan self care activity pada pasien diabetes melitus di Puskesmas Gunung Tua. Jenis penelitian ini kuantitatatif dengan desain penelitian yang digunakan adalah deskriptif korelatif dengan pendekatan cros sectional study. Populasi pada penelitian sebanyak 96 pasien diabetes melitus dengan jumlah sampel 77 responden. Tehnik pengambilan sampel menggunakan random sampling. Pengumpulan data menggunakan kuesioner dengan uji statistik uji chi square. Hasil penelitian signifikan dengan p value=0,001 menunjukkan adanya hubungan antara dukungan keluarga dan kepatuhan self care activity pada penderita diabetes melitus di puskesmas Gunung Tua Padang Lawas Utara. Saran dari penelitian ini pentingnya perilaku perwatan diri (self care) diabetes meliputi diet dan pola makan yang teratur, aktivitas fisik atau olahraga secara rutin, minum obat secara rutin, cek gula darah dan perawatan kaki secara teratur sehingga akan mendapatkan hasil yang optimal. Perlunya keterlibatan dukungan keluarga agar perilaku perawatan diri pasien diabetes melitus optimal.
Formulasi Ekstrak Daun Sirih Hijau ( Piper Betle L) Sebagai Anti Jerawat
Authors: Harahap Cory , Yanti Susi , Nova Ira , Natunna Sofiyah
Published: January 2024

Abstrak
Daun sirih hijau (Piper betle L.) mengandung flavanoid yang cukup baik dalam menghambat pertumbuhan bakteri penyebab jerawat, salah satunya Propionibacterium acnes. Tujuan penelitian ini adalah untuk mengetahui bedak tabur anti jerawat ekstrak daun sirih hijau (Piper betle L.) dapat menghabat pertumbuhan bakteri Propionibacterium acnes, dan untuk mengetahui ekstrak daun sirih hijau (Piper betle L.) dapat diformulasikan menjadi sediaan bedak tabur anti jerawat. Menggunakan metode eksperimental. Hasil penelitian pada uji skrining fitokimia positif mengandung flavanoid, tanin, saponin. Uji organoleptis berwarna hijau dan berbau khas daun sirih hijau. Uji homogenitas sediaan homogen. Uji pH 5,22. Uji daya hambat antibakteri 6,5-10 mm termasuk dalam kategori daya hambat sedang, dimana kategori daya hambat sedang yaitu sebesar 5-10 mm. uji iritasi tidak terdapat reaksi yang menunjukkan adanya iritasi pada responden. Uji hedonik pada sediaan bedak tabur yang paling disukai oleh responden formula sediaan 3 konsenterasi 6%. Kesimpulan dari penelitian ini adalah bedak tabur anti jerawat ekstrak daun sirih hijau (Piper betle L.) dapat menghambat pertumbuhan dari bakteri Propionibacterium acnes dan daun sirih hijau (Piper betle L.) dapat diformulasikan menjadi bedak tabur anti jerawat.
 
Katakunci:Daun sirih hijau, Jerawat, Bedak tabur
 
LOOSE POWDER FORMULATION ANTI-ACNE EXTRACT
GREEN SRIH LEAVES (Piper betle L.)
 
Abstract
Green betel leaf (Piper betle L.) contains flavanoids that are quite good at inhibiting the growth of acne-causing bacteria, one of which is Propionibacterium acnes. The purpose of this study was to determine the anti-acne powder of green betel leaf extract (Piper betle L.) can inhibit the growth of Propionibacterium acnes bacteria, and to determine the green betel leaf extract (Piper betle L.) can be formulated into an anti-acne powder preparation. Using experimental method. The results of the research on phytochemical screening test were positive for flavanoids, tannins, saponins. Organoleptical test is green and smells typical of green betel leaves. Homogeneity test is homogeneous preparation. pH test 5.22. The antibacterial inhibition test of 6.5-10 mm is included in the category of moderate inhibition, where the category of moderate inhibition is 5-10 mm. irritation test there is no reaction that indicates irritation to the respondent. Hedonic test on the preparation of loose powder which is most preferred by respondents in the preparation formula 3 with a concentration of 6%. The conclusion of this study is that anti-acne powder of green betel leaf extract (Piper betle L.) can inhibit the growth of Propionibacterium acnes bacteria and green betel leaf (Piper betle L.) can be formulated into anti-acne powder.
 
Keywords: Green betel leaf, Acne, Loose powder