bluuebunny/crossref_metadata_2025 huggingface dataset:
Nutrition and Gut Health: Preparation and Efficacy of Resistant Starch
Authors: Niu Yulong , Wang Li , Gong Huiyi , Jia Shuqing , Guan Qing , Li Linling , Cheng Hua
Published: February 2025

Resistant starch (RS) refers to starch varieties that resist digestion by human digestive enzymes. Owing to its distinctive physicochemical attributes and functional capabilities, RS has gained a wide range of applications as a dietary fiber and prebiotic. In terms of structure and functions, RS can be categorized into five distinct types: RS1 through RS5. These types offer dietary benefits, contributing to improved colonic health, the modulation of microbial communities, the reduction in gallstone formation, the enhancement of mineral absorption, and alterations in fat oxidation potential. From a technical standpoint, RS can be manufactured through an array of physical, enzymatic, and chemical modifications. This paper presents a comprehensive review of the existing literature, summarizing the classification, structural features, raw material origins, preparation methodologies, and functionalities of RS. Furthermore, new production technologies and applications of RS, such as 3D printing, provide valuable insights.
Plant-Based and Hybrid Patties with Healthy Fats and Broccoli Extract Fortification: More Balanced, Environmentally Friendly Alternative to Meat Prototypes?
Authors: Penalver Josemi G. , Aldaya Maite M. , Villaño Débora , Vírseda Paloma , Beriain Maria Jose
Published: February 2025

Hybrid and plant-based products are an emerging trend in food science. This study aimed to develop three patty prototypes (meat, hybrid, and plant-based) enhanced with vegetable fat replacement and broccoli extract using a soy allergen-free protein matrix treated with high hydrostatic pressure (HHP) and sous vide cooking to create sustainable and nutritious burger alternatives. The samples were evaluated for microbiological safety, proximal composition, physicochemical properties, sensory characteristics, and carbon footprint. The key findings revealed that the plant-based patties had the smallest carbon footprint (0.12 kg CO2e), followed by the hybrid patties (0.87 kg CO2e) and the meat patties (1.62 kg CO2e). The hybrid patties showed increased hardness, cohesiveness, gumminess, and chewiness compared to the meat patties after sous vide treatment. This improvement likely results from synergies between the meat and plant proteins. Regarding the treatments, in all the samples, the highest hardness was observed after the combined HHP and sous vide treatment, an interesting consideration for future prototypes. Sensory analysis indicated that the plant-based and hybrid samples maintained appealing visual and odour characteristics through the treatments, while the meat patties lost the evaluator’s acceptance. Although further improvements in sensory attributes are needed, hybrid patties offer a promising balance of improved texture and intermediate carbon footprint, making them a viable alternative as sustainable, nutritious patties.
Advances in Spray-Drying and Freeze-Drying Technologies for the Microencapsulation of Instant Tea and Herbal Powders: The Role of Wall Materials
Authors: Mazár Júlia , Albert Krisztina , Kovács Zoltán , Koris András , Nath Arijit , Bánvölgyi Szilvia
Published: February 2025

The microencapsulation of tea and herbal extracts is gaining considerable attention in the food industry, particularly in the production of instant powders. This review examines the application of spray-drying and freeze-drying technologies for the encapsulation of bioactive compounds, focusing on the role of wall materials. Over the past two decades, carbohydrate-based (e.g., maltodextrin), gum-based (e.g., gum Arabic), and protein-based (e.g., whey protein isolate) materials have been widely used due to their impact on sensory properties, stability, protection of bioactive compounds, and other critical attributes of encapsulated products. Despite their widespread use, these materials have distinct advantages and limitations, such as cost, availability, and compatibility with different extracts. This review provides a comprehensive analysis of their physical and chemical properties, examines alternative and emerging wall materials (e.g., beta-cyclodextrin, sodium alginate, and inulin), and highlights the potential of combining different materials to optimise encapsulation outcomes. It also identifies current research gaps and future directions to improve the efficacy and quality of encapsulated tea and herbal powders.
Agro-Residues and Sucrose Alternatives in Confectionery Transformation Towards Glucose Spikes Minimization
Authors: Zlatanović Snežana , Laličić-Petronijević Jovanka , Pastor Ferenc , Micić Darko , Dodevska Margarita , Stevanović Milica , Karlović Sven , Gorjanović Stanislava
Published: February 2025

Apple and beetroot pomace flour (APF and BPF), along with two sweeteners, sucrose and a blend of sucrose substitutes (erythritol, stevia, inulin, and fructose), were simultaneously incorporated into three matrices formulated with agar, pectin, or gelatin as gelling agents. The aim was to produce jelly candies with high content of dietary fiber and dietary phenolics, and reduced energy value. The simultaneous incorporation of sucrose substitutes and pomace flour resulted in decrease of Carb:Fiber and Sugar:Fiber Ratio to extremely low values of 2.7–3.4 and 1.3–1.6 respectively, as well as in Energy:Fiber Ratio decrease to 9.2–12.3 kcal/g DF. Relative Antioxidant Capacity Index (RACI), as indicator of antioxidant potential, was calculated by assigning equal weight to Folin–Ciocâlteu, DPPH and FRAP assays applied upon in vitro digestion of 18 formulations of jelly candies. Results obtained for formulations with and without sucrose, as well as with and without APF or BPF, enabled insight into effects of pomace flour addition and sucrose substitution in each gelling matrix on functional properties. The incorporation and the substitution impact on postprandial glucose response were followed in vivo. Their superimposing resulted in glycemic index below 30 and low glycemic load. Efficiency of applied approach in functionalization of confectionery burden with energy and minimization of glucose spike represent an example of agro-residues re-introduction with the highest potential contribution to anti-obesity strategy.
Development and First Validation of the Perceived Young Adult Lockdown Parental Relationship Scale (PYALPRS): An Italian Case Study
Authors: Regnoli Giorgio Maria , Sommantico Massimiliano , Tiano Gioia , Postiglione Jacopo , De Rosa Barbara
Published: February 2025

The COVID-19 pandemic and the confinement experience have significantly affected the relationship between young adults and their parents. The present study focuses on the design and validation of the Perceived Young Adult Lockdown Parental Relationship Scale (PYALPRS), a measure assessing two dimensions of the child–parent relationship associated with the COVID-19 lockdown period: Oppression-Conflict and Closeness-Support. After a phase of construct definition and item design and purification, 100 Italian young adults (M = 24; SD = 3.9) were recruited to explore the factor structure of the scale. Then, a sample of 259 Italian young adults (aged 18–35; M = 24; SD = 3.8) was used to demonstrate the psychometric validity of the scale. The results of our confirmatory factor analysis, which resulted in high goodness of fit scores, support two identifiable factors reflecting the theory-based constructs of the PYALPRS. Moreover, internal consistency and convergent and divergent validity analyses show that the PYALPRS can be considered a reliable and valid instrument. ANOVA demonstrated that there were significant differences between being a cohabitant or single as well as between different perceptions of the home space during lockdown on the Oppression-Conflict dimension, while a larger home space perception was associated with the Closeness-Support dimension.
Why Do Glioblastoma Treatments Fail?
Authors: Rončević Alen , Koruga Nenad , Soldo Koruga Anamarija , Rončević Robert
Published: February 2025

Glioblastoma (GBM) is the most aggressive brain tumor, characterized by high recurrence rates and poor patient outcomes. Treatment failure is driven by multiple factors, including complex tumor heterogeneity, the presence of cancer stem cells, the immunosuppressive tumor microenvironment (TME), and many others. GBM’s heterogeneity underlines its ability to resist therapies and adapt to the TME. The TME, which is highly immunosuppressive and shaped by hypoxia, impairs anti-tumor immunity and limits the efficacy of immunotherapy. The blood–brain barrier (BBB) remains a major obstacle to delivering sufficient drug concentrations to the tumor by restricting the penetration of therapeutic agents. Another problem is the lack of reliable biomarkers to perform better patient stratification or even guide personalized treatments, resulting in generalized therapeutic approaches that do not adequately address GBM complexities. This review highlights the multifactorial nature of GBM treatment failure and highlights the need for a paradigm shift and innovative, personalized strategies. A deeper understanding of tumor biology and advances in translational research will be crucial to developing effective therapies and improving patient outcomes in this devastating disease.
Success Factors in Commercialization of Wing-in-Ground Crafts as Means of Maritime Transport: A Case Study
Authors: Kerem Kristin , Carjova Kristīne , Tapaninen Ulla Pirita
Published: February 2025

The wing-in-ground (WIG) effect occurs when air pressure is created beneath a craft moving close to the ground. The pressure created adds upwards lift, resulting in less need for propulsion for moving forward. Over the years, several companies in various countries have developed wing-in-ground crafts—marine vessels, looking like airplane, that operate using the ground effect. However, no commercial routes are currently in operation using such crafts. This article seeks to identify the critical factors that contribute to the successful commercialization of WIG crafts. The study is composed of a literature review, a company comparison and an analysis of one case study close to successful commercialization. The study indicates that the following actions are critical for the commercial success of a company engaged in WIG operations: engaging community, enhancing R&D, establishing a robust technological system and focusing on safety and compliance. It is also noted that technological readiness itself does not guarantee the successful implementation of WIG crafts on commercial routes.
Bioprinting-By-Design of Hydrogel-Based Biomaterials for In Situ Skin Tissue Engineering
Authors: Douglas Alisa , Chen Yufei , Elloso Margarita , Levschuk Adam , Jeschke Marc G.
Published: February 2025

Burns are one of the most common trauma injuries worldwide and have detrimental effects on the entire body. However, the current standard of care is autologous split thickness skin grafts (STSGs), which induces additional injuries to the patient. Therefore, the development of alternative treatments to replace traditional STSGs is critical, and bioprinting could be the future of burn care. Specifically, in situ bioprinting offers several advantages in clinical applications compared to conventional in vitro bioprinting, primarily due to its ability to deposit bioink directly onto the wound. This review provides an in-depth discussion of the aspects involved in in situ bioprinting for skin regeneration, including crosslinking mechanisms, properties of natural and synthetic hydrogel-based bioinks, various in situ bioprinting methods, and the clinical translation of in situ bioprinting. The current limitations of in situ bioprinting is the ideal combination of bioink and printing mechanism to allow multi-material dispensing or to produce well-orchestrated constructs in a timely manner in clinical settings. However, extensive ongoing research is focused on addressing these challenges, and they do not diminish the significant potential of in situ bioprinting for skin regeneration.
Building Footprint Identification Using Remotely Sensed Images: A Compressed Sensing-Based Approach to Support Map Updating
Authors: Ansari Rizwan Ahmed , Malhotra Rakesh , Ansari Mohammed Zakariya
Published: February 2025

Semantic segmentation of remotely sensed images for building footprint recognition has been extensively researched, and several supervised and unsupervised approaches have been presented and adopted. The capacity to do real-time mapping and precise segmentation on a significant scale while considering the intrinsic diversity of the urban landscape in remotely sensed data has significant consequences. This study presents a novel approach for delineating building footprints by utilizing the compressed sensing and radial basis function technique. At the feature extraction stage, a small set of random features of the built-up areas is extracted from local image windows. The random features are used to train a radial basis neural network to perform building classification; thus, learning and classification are carried out in the compressed sensing domain. By virtue of its ability to represent characteristics in a reduced dimensional space, the scheme shows promise in being robust in the face of variability inherent in urban remotely sensed images. Through a comparison of the proposed method with numerous state-of-the-art approaches utilizing remotely sensed data of different spatial resolutions and building clutter, we establish its robustness and prove its viability. Accuracy assessment is performed for segmented footprints, and comparative analysis is carried out in terms of intersection over union, overall accuracy, precision, recall, and F1 score. The proposed method achieved scores of 93% in overall accuracy, 90.4% in intersection over union, and 91.1% in F1 score, even when dealing with drastically different image features. The results demonstrate that the proposed methodology yields substantial enhancements in classification accuracy and decreases in feature dimensionality.
Hindering Factors and Perceived Needs for the Decision Making of Advanced Directives Among People with Dementia and Their Families
Authors: Lin Hsiu-Ching , Lu Yu-Fang , Yeh Ching-Hsueh , Wang Jy-Jing , Yang Ya-Ping
Published: February 2025

Making advanced directives is challenging in Asia. The hindering factors and perceived needs for advanced directives for people with dementia and their families have not been fully explored in Taiwan. In this study, we aimed to identify the barriers and perceived needs of people with mild dementia and the families of people with dementia within the cultural context of Taiwan for advanced directives. A qualitative descriptive design with purposive sampling and content analysis was used to collect and analyze the data. Thirteen people with mild dementia and thirty-two families of people with dementia were recruited. Our findings indicated that the hindering factors for people with mild dementia and the families of people with dementia to make advanced directives included “talking about death is a taboo”, “the timing is not right”, “cultural values of filial piety”, “male protagonist’s social status”, and “insufficient information on advanced directive”. The perceived needs for participants in making advanced directive decisions were “a wish to die without suffering”, “wanting to rely on others to make a decision”, and “an increased awareness of information”. This research offers valuable insights into the barriers and needs related to advanced directives for people with mild dementia and the families of people with dementia in Taiwan. These findings address the identified challenges and needs to develop effective solutions to help healthcare providers to better facilitate the decision-making process for advanced directives.
A Bibliometric Analysis of Healthcare Intervention-Related Studies Reporting Patient and Public Involvement and Engagement
Authors: Lu Wenze , Li Yan , Montayre Jed , Li Mengqi , Ho Ka Yan , Li Jiaying , Yorke Janelle
Published: February 2025

Background/Objectives: Patient and public involvement and engagement (PPIE) has gained global recognition as an innovative healthcare research practice. PPIE engages end-users throughout the research process, improving intervention effectiveness, resource efficiency, and user satisfaction. Despite its increasing inclusion in studies, comprehensive bibliometric reviews of healthcare intervention-related studies reporting PPIE are scarce. This study aims to conduct a bibliometric analysis of healthcare intervention-related studies reporting PPIE in recent decades to identify key worldwide bibliometric features, themes, and trends. Methods: The analysis includes 10,624 relevant English articles published in the Web of Science (WoS) Core Collection up to 26 November 2024. Search terms were selected based on PPIE conceptualization, interventional types, and related healthcare terms. Using WoS descriptive analysis and CiteSpace, we examined bibliometric features and identified major international themes and trends. Results: There has been a significant increase in the number of healthcare intervention-related studies reporting PPIE over the past five years, especially from the United States and the United Kingdom, with a recent rise in Asia. However, cross-national collaboration remains limited. Key research themes identified include “community participation”, “health equity”, “coronary heart disease”, “web-based patient empowerment”, “mental illness”, and “obesity prevention”, with growing interest in “mobile health” and “digital health”. Conclusions: This study provides a comprehensive and up-to-date overview of the bibliometric characteristics and evolving trends in healthcare intervention-related studies reporting PPIE. It highlights global regions with limited PPIE implementation, suggests pathways for further development, and identifies key research themes. The study offers researchers and practitioners valuable insights into tracking PPIE trends in healthcare interventions and fostering collaborations on evidence-based PPIE studies with leading scholars and institutions worldwide. Additionally, the findings drive innovations aimed at improving patient and public healthcare outcomes.
Prognostic Factors for Responders of Home-Based Pulmonary Rehabilitation—Secondary Analysis of a Randomized Controlled Trial
Authors: Kim Chul , Choi Hee-Eun , Rhee Chin Kook , Lee Jae Ha , Oh Ju Hyun , Song Jun Hyeong
Published: February 2025

Background: Home-based pulmonary rehabilitation (PR) is an effective alternative to center-based PR. However, not all participants exhibit sufficient therapeutic improvement, highlighting the need to identify appropriate candidates to maximize cost-effectiveness. This study aimed to identify the factors associated with favorable outcomes in home-based PR, focusing on the role of digital therapeutics (DTx). Methods: This secondary analysis used data from a randomized controlled trial. Participants with chronic respiratory disease (CRD) were divided into responders and non-responders based on a change in 6 min walk distance (6MWD) and patient-reported outcome measures (PROM) representing dyspnea and health-related quality of life. Factors such as baseline 6MWD, DTx use, and pulmonary function were analyzed for their predictive value in improving 6MWD and PROM scores. Results: Responders, particularly those using DTx, showed significantly greater improvements in 6MWD than non-responders. Participants with a baseline 6MWD under 500 m demonstrated a higher likelihood of exceeding the minimum clinically important difference in 6MWD. DTx use strongly predicted improvements in both 6MWD and PROM scores. The baseline diffusing capacity of the lungs for carbon monoxide was also a significant factor influencing improvements in the modified Medical Research Council scale. Conclusions: Responders to 8-week program of home-based PR exhibited a relatively lower baseline health status. Encouraging participants with poorer baseline health could improve adherence to PR and enhance cost-effectiveness. Additionally, improvements in 6MWD and PROM scores were associated with the use of DTx. Considering the functions of DTx, proper supervision for home-based exercise may be crucial for achieving optimal outcomes.
Exploring a Co-Designed Approach for Healthcare Quality Improvement—Learning Through Developmental Evaluation
Authors: Kovacs Burns Katharina , George Marian
Published: February 2025

Background: Healthcare setting teams were challenged to understand how and what to measure regarding healthcare quality improvement (HQI), who should be involved, and what approach to apply. We aimed to determine if a generic co-design approach involving patients/families, multi-disciplinary care providers, and other staff was feasible to apply for HQI across diverse care settings. Developmental evaluation embedded in the co-design approach would determine its effectiveness, challenges, and other experiences across care settings and teams. Methods: Twenty-two acute and community care settings agreed to participate in applying a phased co-design approach to their HQI initiatives, including developmental evaluation. Each care setting team received co-design orientation and support. Semi-structured interviews and focus groups were conducted with patient/family advisors (PFAs) and care setting staff/care providers to gather their experiences with the co-design approach applied to their phased HQI work. Transcripts were thematically analyzed and triangulated with observation notes of care setting team discussions. Experiences were gathered from 17 PFAs and 68 staff/care providers across the 22 participating healthcare settings. Results: Themes for the orientation and each phase emphasized the importance of participants’ understanding, engagement, and ongoing open communication throughout the HQI co-design process. The orientation was viewed as key to facilitating good outcomes. Participants valued working together, gathering real-time experiences to “make a difference”, and having PFA voices involved in co-designing the HQI initiatives. Challenges were identified, including time commitment. Conclusions: Based on the overall developmental evaluation findings, there was consensus that a generic co-design of HQI initiatives was effective, feasible, and sustainable across care settings.
Bosutinib-Induced Pleural Effusion—Class Effect and Cross-Intolerance to All Tyrosine Kinase Inhibitors
Authors: Vojjala Nikhil , Sami Hizqueel A. , Kotla Nikhil Kumar , Peshin Supriya , Goyal Kanika , Kondaveety Soumya , Prabhu Rishab Rajendra , Krishnamoorthy Geetha
Published: February 2025

Introduction: Tyrosine kinase inhibitors (TKIs) serve as the backbone in the management of chronic myelogenous leukemia and Philadelphia-positive Acute lymphoblastic Leukemia (Ph+ve ALL). With the growing use of TKIs, there has been an increase in adverse events related to these agents. Hereby, we present elderly women with Ph+ve ALL who developed recurrent pleural effusion, which was managed by switching the TKI and highlighting pleural effusion due to a third-generation TKI Bosutinib, adding to the minimal available literature. Case Description: Our patient is a 79-year-old female with Ph+ve ALL diagnosed in 2015 and started on treatment. She is also on TKI maintenance initially with Imatinib later shifted to second-generation TKIs. She started developing worsening dyspnea related to pulmonary toxicity related to TKI in the form of pleural effusion. Pleural effusion was initially managed with diuretics, later requiring thoracocentesis. Because of persistent pleural effusion, she was changed to multiple TKIs and finally started on Bosutinib. She even developed progressive pleural effusion while on Bosutinib which is managed by thoracocentesis. Conclusions: Through this case report, we would like to highlight refractory recurrent pleural effusion caused by bosutinib adding to the minimal available literature. In addition, we highlight the various treatment options in patients having cross-intolerance to various TKIs, especially pulmonary toxicity, and ponatinib might be a suitable option in such cases.
“Les Malcontents” and the Monarchomach Treatises: The Aristocratic Justification of Revolt and the Ideology of Popular Sovereignty in 1570s France
Authors: Sălăvăstru Andrei Constantin
Published: February 2025

Intended to destroy the aristocratic leadership of the Huguenots, the massacre of St. Bartholomew galvanized instead the opposition to a monarchy seen now not only as tyrannical, but also treacherous. The Huguenots started exploring various ways to check and even depose a hostile monarch, in the so-called monarchomach treatises. But the massacre also led to the formation of a faction of moderate Catholic aristocrats, “les malcontents”, who cooperated with the Huguenots against a monarchy that, in their opinion, had committed a major breach of trust. Both the Huguenots and the malcontents proposed their own constitutional theories, aimed at limiting the power of the monarchy: the former argued in favor of a form of popular sovereignty, which would have turned the king into something akin to a first magistrate of his kingdom, while the latter put forward ideas that preserved the king as the undisputable head of the political pyramid, but argued in favor of the right to revolt, in the name of the same king, for the sake of the “public good”. The aim of this paper is to examine both the differences and the common ground between these two political models, as they are reflected in the propaganda issued by the Huguenots and the leaders of the malcontents during the 1570s.
Study of Zhejiang Tangerine E-Commerce Reviews Based on Natural Language Processing
Authors: Yuan Leiming , Liu Haoyang , Fu Fangfang , Liu Yimin , Zuo Xiaoyu , Li Limin
Published: February 2025

In recent years, the global economy has experienced significant shifts, leading to a trend of consumption downgrading. Amid economic pressures and uncertainties, consumers are increasingly turning to cost-effective shopping methods. The COVID-19 lockdowns further accelerated the growth of e-commerce platforms, presenting both opportunities and challenges for sales. Electronic commerce has played a crucial role in enhancing the sales of agricultural products with regional characteristics in China, thereby opening new channels for farmers. This article utilizes tangerines, particularly popular in Zhejiang Province, as a case study to explore e-commerce reviews and assist merchants in delivering more satisfactory products. The analysis of tangerine reviews revealed that customers primarily focused on the taste, service, quality, and price. By applying the latent Dirichlet allocation (LDA) topic model, comments were categorized into four themes: ‘quality’, ‘service’, ‘price’, and ‘flavor’, with key terms identified for each theme. Through sentiment analysis using SnowNLP and bidirectional encoder representations from transformers (BERT), it was found that online shoppers generally expressed positive sentiment toward tangerines. However, there was also some negative feedback. These findings are of paramount importance for businesses aiming to meet consumer demands. The study acknowledges certain limitations including the reliability of data mining and the accuracy of Chinese corpus analysis. Future research could benefit from employing more precise language models to enhance the analysis, ultimately improving the consumer shopping experience and aiding businesses in service improvement.
Biological Control Agents in Greenhouse Tomato Production (Solanum lycopersicum L.): Possibilities, Challenges and Policy Insights for Western Balkan Region
Authors: Ivezić Aleksandar , Popović Tamara , Trudić Branislav , Krndija Jovan , Barošević Tijana , Sarajlić Ankica , Stojačić Isidora , Kuzmanović Boris
Published: February 2025

Greenhouse tomato production holds strong economic importance in the Western Balkan (WB) countries, driven by escalating market demands. To align with global food safety trends, systematic advancements in cultivation practices and pest management strategies are essential for the WB region. Conventional reliance on chemical pesticides as the primary pest control method has led to various adverse outcomes, impacting pest management effectiveness and reducing product quality. The impact of climate change on agricultural productivity further emphasizes the need for environmentally friendly solutions in pest protection. To address these challenges and mitigate the detrimental effects of chemical pesticides, this review explores sustainable, alternative solutions, like biological control agents (BCAs), for tomato pest control in the WB region. With biological control gaining recognition as an effective approach, yet remaining underutilized in the WB, this review provides a thorough analysis of the most effective, commercially available BCAs, critically evaluating their advantages and limitations. Widespread BCA application could support the expansion of the tomato production sector while fostering sustainable agricultural practices. Furthermore, the review explores the role of BCAs within Integrated Pest Management (IPM) frameworks in tomato production, emphasizing their alignment with Sustainable Development Goals (SDGs), particularly for countries aiming for EU integration.
The Impacts of Light Interception on Yield and Kernel Parameters in Hazelnut Production
Authors: Gutiérrez-Gamboa Gastón , Araya-Alman Miguel , Zúñiga-Sánchez Mauricio , González Marcelo , Lisperguer Fernández María José , Romero-Bravo Sebastián
Published: February 2025

Chile has become a significant producer of hazelnuts (Corylus avellana L.) due to its favorable conditions for off-season production to meet global market demand. Efficient light interception is crucial for optimizing the hazelnut yield and quality, as it directly influences the plant’s photosynthetic capacity. The goal was to assess the impact of intercepted photosynthetically active radiation (PAR) at upper, middle, and lower canopy levels in hazelnut trees grown at different planting densities and the canopy management of yield and quality parameters. The percentage of vain, sucker, double, bug, and healthy hazelnuts, including the number of fruits, total fruit weight, and grams per fruit was evaluated. The upper canopy levels intercepted the highest PAR, significantly enhancing the number and weight of fruits. The lower canopy levels received considerably less PAR, reducing the productivity. Production pruning in dense planting systems (5 × 3 m) improved the light penetration compared to a less dense planting density (5 × 6 m). Strong positive correlations were observed among PAR, fruit weight, and fruit number, emphasizing the importance of the light distribution within the canopy in hazelnut production. Production pruning and optimized planting densities are essential for maximizing light interception, improving yield, and ensuring high-quality hazelnut production.
Enhancing Drought Tolerance in Lettuce: The Efficacy of the Seaweed-Derived Biostimulant Cytolan® Stress Applied at Different Growth Stages
Authors: Velasco-Clares Daniel , Navarro-León Eloy , Izquierdo-Ramos María José , Blasco Begoña , Ruiz Juan Manuel
Published: February 2025

Water stress is one of the foremost global abiotic stressors limiting agricultural productivity. Biostimulants and bioactive compounds are emerging as promising tools to enhance crop stress tolerance. This study investigates the effects of Cytolan® Stress, a novel seaweed-derived biostimulant, on the water stress tolerance of lettuce plants. Three application strategies were evaluated: priming, where the biostimulant is applied before the onset of stress to prepare the plants for adverse conditions; buffering, involving application at the onset of stress to mitigate its immediate effects; and detoxifying, where the biostimulant is applied after stress to aid in plant recovery. Biomass, stress-related parameters, antioxidant activity, osmoprotectant levels, and photosynthesis-related metrics were analyzed to elucidate its potential mechanisms of action. The results demonstrated that Cytolan® Stress in priming and buffering applications significantly improved water stress tolerance, reducing biomass loss from 45% to only 25%. Moreover, the detoxifying treatment was the most effective, as plants showed biomass values similar to those of the control plants. The biostimulant reduced oxidative stress indicators while enhancing antioxidant defenses, including ascorbate (AsA)-glutathione (GSH) cycle, antioxidant compounds, and enzyme activities. In addition, Cytolan® Stress preserved photosynthesis performance under water stress conditions. These findings highlight the potential of Cytolan® Stress to mitigate drought stress effects in lettuce, offering broader implications for crop tolerance and resilience under water-limited conditions. Further studies are recommended to explore its efficacy across different crops and stress scenarios.
Tailored Spectral Lighting Enhances Growth and Photosynthetic Efficiency of Wasabia japonica
Authors: Bae Sunghwan , Ahn Hyoseong , Kim Minha , Park Sangun , Kim Yongduk
Published: February 2025

This study evaluated the effects of various supplemental lighting conditions on the growth and rhizome production of Wasabia japonica, a high-value crop. Its cultivation poses challenges due to specific environmental requirements, including cool temperatures and high humidity. By tailoring light spectra, we aimed to optimize photosynthetic efficiency and biomass accumulation. Seedlings (cv. ST1) were grown in a controlled plant factory (18 ± 2 °C, 90 ± 5% RH) using a Yamazaki tri-leaf nutrient solution (EC 1.5–2.0 dS/m, pH 5.8–6.8). Lighting was designed to convert blue (450 nm) to red (630–680 nm) wavelengths at 70–40% ratios, maintaining a photosynthetic photon flux density (PPFD) of 50 ± 5 μmol·m⁻2·s⁻1 during a 12 h photoperiod. In the greenhouse, supplemental lighting (40% blue-to-red conversion) was applied for 4 h daily to complement natural light. After 140 days, the optimized 40% blue-to-red conversion lighting significantly improved plant length (34.4 cm), leaf length (15.3 cm), and photosynthetic rates (2.21 μmol·m⁻2·s⁻1). When tested in the greenhouse, it increased rhizome fresh weight to 75.6 g compared to 30.0 g under natural light. These results indicate that supplemental lighting with specific spectral ratios and controlled intensities can enhance photosynthesis and rhizome production, providing a sustainable approach to wasabi cultivation.
The Use of Fluorescent Organic Matter as a Natural Transit Time Tracer in the Unsaturated Zone of the Fontaine De Vaucluse Karst System
Authors: Serène Leïla , Mazzilli Naomi , Batiot-Guilhe Christelle , Emblanch Christophe , Babic Milanka , Dupont Julien , Simler Roland , Blanc Matthieu
Published: February 2025

The fluorescence index called the Transit Time index (TTi) is based on the fluorescence of natural organic matter in order to qualitatively assess the transit time of karst groundwater, using springs affected by human activities. This study aims to further evaluate the potential of fluorescent compounds as a natural tracer of transit time when applied to unsaturated zone flows with natural catchments, in contrast to the first study. For this purpose, a bi-monthly sampling of one year of monitoring for organic matter fluorescence, TOC, major elements and water-stable isotopes was performed. A conceptual model of the sources and fates of fluorescent compounds is built, emphasizing the allochthonous origin of humic-like C compounds, and the autochthonous production of humic-like M and protein-like compounds within the unsaturated zone. Fluorescent compound intensity interpretation according to this model reveals consistent relative transit times with flow behavior and also provides complementary information. The results also show the TTi’s ability to summarize fluorescent compounds, its consistency with relative transit time, and its higher sensitivity as compared to other natural tracers. However, prior to its use, a thorough assessment of soil organic matter, microbial activity, and potential anthropogenic contamination is required, encouraging interdisciplinary collaboration between hydrogeologists, microbiologists and soil scientists.
Hard to Borrow vs. Easy to Borrow: Insights from Japan’s Centralized Lendable Stock Market
Authors: Khan Mostafa Saidur Rahim
Published: February 2025

This study examines stock borrowing costs in Japan’s centralized lendable stock market, focusing on differences between ‘hard-to-borrow’ and ‘easy-to-borrow’ stocks over six months of daily data. This study employs a comprehensive methodology to examine metrics such as the short interest ratio, borrowing costs, institutional ownership, price-to-book value ratio, and new stock borrowing patterns. Regression models are utilized to explore the relationships between these factors and borrowing costs. The findings reveal that ‘hard-to-borrow’ stocks are associated with higher short interest ratios, borrowing costs, price-to-book ratios, and turnover but exhibit lower institutional ownership compared to ‘easy-to-borrow’ stocks. Notably, institutional ownership negatively correlates with borrowing costs across both categories, while the short interest ratio positively correlates with borrowing costs only for ‘hard-to-borrow’ stocks. Contrary to expectations, ‘hard-to-borrow’ stocks do not underperform despite elevated borrowing expenses, suggesting that these costs do not deter short selling activities in the Japanese market. The findings of this study offer key implications for investors and regulators. For investors, understanding the factors influencing borrowing costs aids in optimizing short-selling strategies. For regulators, the results highlight the role of centralized lendable stock markets in enhancing pricing efficiency without hindering trading activities.
Targeting Ferroptosis/Nrf2 Pathway Ameliorates AlCl3-Induced Alzheimer’s Disease in Rats: Neuroprotective Effect of Morin Hydrate, Zeolite Clinoptilolite, and Physical Plus Mental Activities
Authors: Abu-Elfotuh Karema , Mahran Yasmin , Bayoumie El Gazzar Walaa , Youssef Heba S. , Hamdan Ahmed M. E. , Albalawi Tariq Mohammed , Alsunbul Maha , ALQahtani Reem , Mohammed Asmaa A.
Published: February 2025

Alzheimer’s disease (AD) is a significant health challenge in the 21st century. In spite of the approval of many new disease-modifying therapies for AD, the clinical advantages of these new treatments are less certain. Aim: This investigation was intended to determine the potential neuroprotective impact of morin hydrate (MH), zeolite clinoptilolite (ZC), and/or physical and mental activities (PhM) on an aluminum chloride (AlCl3)-induced AD rat model. Methods: Male Sprague Dawley rats were randomly allocated into seven groups. Group I was the control group. Groups II–VII were treated with AlCl3 for 5 weeks. Groups III–VII were tested for the effects of MH, ZC, and/or PhM. Biochemical, brain histopathological, and behavioral studies were performed. Results: PhM, MH, and ZC combined therapy exhibited a significant neuroprotective effect demonstrated by corrected catecholamines and tau and β-amyloid levels, as well as the antioxidant and anti-ferroptotic effects probably through Nrf2/HO-1/GPX4 and ACSL4 signaling pathways. In addition, combined therapy counteracted the inflammatory responses through modulating the TLR4/NF-κβ/NLRP3 inflammasome expression. Moreover, combined therapy groups showed the maximum improvement of both APOE4/LRP1 and Wnt3/β-catenin/GSK-3β signaling expressions. Conclusion: This research highlights the neuroprotective impact of MH and ZC plus PhM against AlCl3-induced AD via modulation of Nrf2/HO-1/GPX4, TLR4/NF-κβ/NLRP3, APOE4/LRP1, and Wnt3/β-catenin/GSK-3β signaling pathways. It is the first to point out the inclusion of ferroptosis-Nrf2/inflammasomes cross-talk in the neuroprotection mechanism of MH/ZC against the AlCl3-mediated AD model.
Two Are Better than One: The Bi-Specific Antibody Mosunetuzumab Reveals an Improved Immune Response of Vγ9Vδ2 T Cells Targeting CD20 in Malignant B Cells in Comparison to the Mono-Specific Antibody Obinutuzumab
Authors: Marischen Lothar , Fritsch Jürgen , Ilic Jovana , Wahl Laura , Bertsch Thomas , Knop Stefan , Bold Anna
Published: February 2025

In treating cancer, immunotherapy has been established as a later-line treatment option in clinical practice. That includes stem cell transplantation, modified or activated immune cells, and antibodies directed against aberrant cells. As an unconventional immune cell subgroup, γδ T cells have been shown to provide effects against malignant cells. They exhibit an MHC-independent activation process, which could diminish graft-versus-host disease after an adoptive transfer of allogeneic cells. Over the last years, the efficacy of therapeutic antibodies has been improved. As a bi-specific antibody, mosunetuzumab binds to both CD3 and CD20, thereby providing close proximity between effector and target cells. Here, we set out to analyze the efficiency of γδ T cells’ anti-tumor effects in combination with mosunetuzumab vs. the monoclonal anti-CD20 antibody obinutuzumab. Mosunetuzumab revealed improved responses of γδ T cells regarding their expression of IFN-γ and CD107a and their cytotoxicity towards malignant B cells from lymphoma B cell lines. In comparison to obinutuzumab, mosunetuzumab led to an equivalent or enhanced cytotoxicity against B cell lymphoma cell lines and primary patient samples, where this effect was even more prominent. In summary, we consider the combination of stimulated γδ T cells and mosunetuzumab to be a promising therapeutic approach for future clinical trials.
Unveiling the Multitarget Potential of a Rare Caffeoyl Ester from Artemisia capillaris for Diabetes Mellitus: An Integrated In Vitro and In Silico Study
Authors: Islam Md. Nurul , Ha Manh Tuan , Min Byung-Sun , Choi Jae Sue , Jung Hyun Ah
Published: February 2025

As a part of our ongoing search for bioactive constituents of Artemisia capillaris, we isolated 4-O-caffeoyl-2-C-methyl-d-threonic acid (PPT-14). This is a rare caffeic acid ester derivative that is reported here for the first time in the Artemisia species, which is the third occurrence in any plant species worldwide. In this study, we evaluated the anti-diabetic potential of PPT-14 using in vitro and in silico approaches. PPT-14 demonstrated significant inhibitory activity against two crucial enzymes linked to diabetes progression and complications: protein tyrosine phosphatase 1B (PTP1B) and aldose reductase (AR). These had IC50 values of 64.92 and 19.50 µM, respectively. Additionally, PPT-14 exhibited free radical scavenging activity with 2,2-diphenyl-2-picrylhydrazyl (IC50 14.46 µM). Molecular docking and 200 ns molecular dynamics simulations confirmed that there were stable binding interactions with the key residues of PTP1B and AR, highlighting strong affinity and dynamic stability. Pharmacokinetic analyses revealed favorable water solubility, adherence to Lipinski’s Rule of Five, and minimal interactions with cytochrome P450 enzymes, indicating the drug-like potential of PPT-14. Toxicity studies confirmed its safety profile, showing no genotoxicity, hepatotoxicity, or significant toxicity risks, with an acceptable oral LD50 value of 2.984 mol/kg. These findings suggest that PPT-14 could be a promising multitarget lead compound for ameliorating diabetes and its associated complications.
The Human Superficial Fascia: A Narrative Review
Authors: Fede Caterina , Clair Claudia , Pirri Carmelo , Petrelli Lucia , Zhao Xiaoxiao , Sun Yunfeng , Macchi Veronica , Stecco Carla
Published: February 2025

In recent years, the interest in the comprehension of the fasciae has significantly grown, together with the necessity of finding a consensus for a terminology of the fasciae in the research and clinical fields. Furthermore, it is becoming necessary to categorize the various types of fascia (superficial, deep, visceral, neural) since they possess different anatomical characteristics, and are implicated in different pathophysiological pathways. While in the past we have described the deep/muscular fascia, the aim of this work is to summarize and catalog the information relating to the human superficial fascia (thickness, cellular end extracellular matrix component, innervation, vascularization).
Exploring the Immunological Aspects and Treatments of Recurrent Pregnancy Loss and Recurrent Implantation Failure
Authors: Garmendia Jenny Valentina , De Sanctis Claudia Valentina , Hajdúch Marián , De Sanctis Juan Bautista
Published: February 2025

Recurrent pregnancy loss (RPL) is defined as the occurrence of two or more consecutive pregnancy losses before 24 weeks of gestation. It affects 3–5% of women who are attempting to conceive. RPL can stem from a variety of causes and is frequently associated with psychological distress and a diminished quality of life. By contrast, recurrent implantation failure (RIF) refers to the inability to achieve a successful pregnancy after three or more high-quality embryo transfers or at least two instances of egg donation. RIF shares several causative factors with RPL. The immunological underpinnings of these conditions involve alterations in uterine NK cells, reductions in M2 macrophages and myeloid-derived suppressor cells, an increased Th1/Th2 ratio, a decreased Treg/Th17 ratio, the presence of shared ≥3 HLA alleles between partners, and autoimmune disorders. Various therapeutic approaches have been employed to address these immunological concerns, achieving varying degrees of success, although some therapies remain contentious within the medical community. This review intends to explore the immunological factors implicated in RPL and RIF and to analyze the immunological treatments employed for these conditions, which may include steroids, intravenous immunoglobulins, calcineurin inhibitors, anti-TNF antibodies, intralipid infusions, granulocyte colony-stimulating factor, and lymphocyte immunotherapy.
Statins as Secondary Preventive Agent to Limit Breast Cancer Metastatic Outgrowth
Authors: Atale Neha , Wells Alan
Published: February 2025

Metastasis is a leading cause of mortality in breast cancer, as metastatic disease is often aggressive and resistant to conventional treatments. Cancer cells that spread to distant organs can enter a dormant phase for extended periods, sometimes years or decades. During this dormant phase, cancer cells avoid immune and pharmacological response. Thus, new approaches are needed to prevent these disseminated cells from becoming lethal cancers. Statins are known inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase that have been extensively used in patients with cardiovascular diseases to lower cholesterol. However, recent research has demonstrated their potential in anticancer therapies. Epidemiological evidence suggests that statins are associated with a reduction in breast cancer-specific mortality, although they do not appear to affect the incidence of primary tumors. In this review, we discuss the role of statins in metastasis and dormancy, their cytocidal and cytostatic effects and their interactions with different cell types in the tumor microenvironment. The exact mechanisms by which statins reduce mortality without influencing primary tumor growth remain unclear, also warranting further investigation into their potential role in metastasis and tumor dormancy, which could ultimately help patients to improve survival and quality of life.