bluuebunny/crossref_metadata_2025 huggingface dataset:
Patriotism and connection with the Homeland of the Russian Roslik family in Uruguay
Authors: Moseykina Marina N. , Saravia Biancimano J. A
Published: July 2024

Based on archival and published sources, this article reconstructs the fate of the Roslik family — a group of sectarian Christians who resettled as a whole colony from the Voronezh province to the Uruguayan city of San Javier in 1913. In this context, the national and social composition of the migrants, representing two waves of emigration, is presented, along with an examination of the adaptation process. The patriotic stance of the Roslik family and the entire colony towards the USSR during the Second World War is elucidated.
Volga Germans in modern Argentina. Issues of preserving of language, culture and ethnic identity
Authors: Litzenberger Olga
Published: July 2024

The phenomenon of ethnic identity and unique cultural heritage of the Volga Germans of Argentina considered in the article is multidimensional. The author analyzes it on the example of the Entre Rios province. In September 2023 the author conducted an expert survey in 15 localities of the province. The information obtained as a result of the survey is grouped in the article into several thematic blocks: ethnic identity; dialect and German language learning; activities of German national-cultural associations to preserve intangible cultural heritage, forecasts and development trends.
“Plowing in the sea”. The Russian Revolution and Latin America
Authors: Reynoso Jaime I.
Published: July 2024

The review analyzes the book "The Russian Revolution and Latin America: 1917 and Beyond," edited by Carlos Miguel Herrera and Eugenia Palieraki. It highlights the diversity of responses to the Russian Revolution, ranging from enthusiasm to rejection, and criticizes the dichotomous view of this event in traditional historiography. The book seeks to understand how the Russian Revolution impacted Latin American societies and challenges the Eurocentric view of the event. Specific cases are examined, such as the development of the Argentine Communist Party, the Communist Party of Mexico and tensions between the Comintern and the Communist Party of Chile. The relationship between the Russian Revolution and the Chilean left is also studied, as well as the impact of communism on the family life of militants. The work concludes with an essay on the "posterity" of the Russian Revolution in Latin America, suggesting that its legacy continues to resonate in the region and contributes to the debate on the role of socialist left in present and future struggles.
Quantum-chemical calculations of the enthalpy of formation for 5/6/5 tricyclic tetrazine derivatives annelated with nitrotriazoles
Authors: Volokhov V. M. , Parakhin V. V. , Amosova E. S. , Volokhov A. V. , Lempert D. B. , Zyubina T. S.
Published: July 2024

The paper presents the study of the calculated physicochemical properties of new high-energy 5/6/5 tricyclic structures, which are 1,2,3,4- or 1,2,4,5-tetrazines, fused with a pair of 1H-1,2,4-, 4H-1,2,4- or 1H-1,2,3-triazoles. Values of the enthalpy of formation in the gaseous phase have been determined by high-performance quantum-chemical calculations (within Gaussian 09 program package) using various methods for solving the stationary Schrödinger equation, including G4, G4MP2, ωB97XD/aug-cc-pVTZ, CBS-APNO, CBS-QB3, CBS-4M, B3LYP/6-311+G(2d,p), M062X/6-311+G(2d,p). The results of calculations obtained by the methods of atomization and isogyric reactions have been analysed. Various calculation methods have been compared in terms of accuracy and time consumption.
Effect of organic corrosion inhibitors on the kinetics of the cathodic hydrogen evolution reaction on steel in a sulfuric acid solution
Authors: Avdeev Ya. G. , Nenasheva T. A. , Luchkin A. Y. , Marshakov A. I. , Kuznetsov Yu. I.
Published: July 2024

The kinetics of hydrogen cathodic reduction on low-carbon steel in a sulfuric acid solution containing a mixture of quaternary ammonium salts (catamine AB) and 3-substituted 1,2,4-triazole (IFKhAN-92 inhibitor) has been studied. The main rate constants of the stages of evolution of gaseous hydrogen and the permetion of hydrogen atoms into the metal are determined. It is shown that these substances reduce the reaction rate of the discharge of H+ ions, change the ratio between the concentrations of H atoms on the surface and in the phase of the metal, and, as a result, reduce the amount of hydrogen absorbed by steel. The most effective inhibitor of corrosion and hydrogenation of steel is IFKhAN-92, due to the formation of a polymolecular protective layer of the inhibitor on the metal surface. The data of X-ray photoelectron spectroscopy of the steel surface show that the protective layer has a thickness of no more than 4 nm and consists of IFKhAN-92 molecules associated with the steel surface by chemical interaction, and inside the layer by physical interaction.
Kinetic modeling of the effect of the conditions of conjugate oxidation of propane and ethylene on the yield of propylene
Authors: Arsentev S. D. , Davtyan A. H. , Manukyan Z. H. , Tavadyan L. A. , Strekova L. N. , Arutyunov V. S.
Published: July 2024

The study of the oxidation of propane-ethylene mixtures by numerical kinetic modeling allowed us to establish that in the range of 400–600 oC with an increase in the conversion of propane with an increase in temperature, the selectivity of propylene formation passes through a maximum, the position of which depends on the concentration of ethylene in the initial mixture. The addition of ethylene to the initial mixture leads to a reduction in propane consumption and an increase in the selectivity of propylene formation. The conditions under which ethylene introduced into the initial mixture is not consumed during the process are determined, so formally it can be considered as a catalyst, and the process of propane oxidation as proceeding in a pseudo-catalytic regime.
Influence of self-heating on the kinetics of an exothermal reaction under isothermal conditions
Authors: Korsunskiy B. L. , Samoilenko N. G.
Published: July 2024

Estimates have been made showing that when an exothermal reaction proceeds in an isothermal medium (in a thermostat), self-heating occurs, the values of which are determined by the competition between the processes of heat input and heat removal. Relationships are obtained that make it possible to estimate the deviation of the observed values of the kinetic parameters from the true values and to reveal the influence of various parameters on these deviations.
Dependence of the TNT equivalent of an underwater explosion on the content of aluminum hydride in the energy material
Authors: Makhov M. N.
Published: July 2024

The results obtained show that the addition of aluminum (Al) and aluminum hydride (AlH3) to the explosive significantly increases the heat of explosion and the TNT equivalent (TE) of an underwater explosion. The compositions with AlH3 are inferior to the Al-containing counterparts in the heat of explosion. However, the formulations with AlH3 have the advantage in terms of the number of moles of gaseous products. Replacing Al with AlH3 weakly affects the TE in terms of the energy of gas bubble, while the TE in terms of the energy of shock wave is higher for the mixtures with AlH3. The latter is especially noticeable in the case of the explosive with a positive oxygen balance. However, the compositions with AlH3 are inferior to the Al-containing mixtures in the volumetric TE.
Features of structural formations in butadiene-nitrile rubber films under the action of a directed electric field
Authors: Shibryaeva L. S. , Komova N. N. , Chizhenok V. A. , Boginskay I. A.
Published: July 2024

The influence of the direction of a constant electric field on the properties and structural features of thin films of nitrile rubber (NBR) formed on copper substrates from a solution in chloroform has been studied. It is shown that the greatest effect in the modification of properties associated with structural transformations in the process of formation of elastomer films occurs on the negative electrode - the cathode, when the electric field lines are directed to the electrode surface. The method of differential scanning calorimetry shows the structural differences between films formed outside and in the presence of electric fields. The films formed in the fields have different electrical characteristics from the films formed outside the field. IR spectroscopy methods confirmed the structuring and cyclization of BNKS molecules on the surface of the film formed on the cathode.
Effect of the formation method of ZnO–In2O3 composites on their structural characteristics and conductivity
Authors: Ikim M. I. , Spiridonova E. Y. , Gromov V. F. , Gerasimov G. N. , Trakhtenberg L. I.
Published: July 2024

Composites based on indium oxide containing different amounts of zinc oxide were synthesized by hydrothermal and impregnation methods. The phase composition, structure, and specific surface of the obtained composites were studied by various physicochemical methods. The electrophysical properties of composites synthesized by different methods are compared. It is shown that the method of formation has a significant effect on the structural characteristics of the composites, which in turn leads to the implementation of various conduction mechanisms.
Reaction operator in the main equation of spin chemistry
Authors: Purtov P. A.
Published: July 2024

In our previous works, using an exactly solvable model as an example, we demonstrated the validity of the reaction operator in the basic equation of spin chemistry. However, in these works, only recombination from the singlet state was considered. Although the general relations allowed us to consider the general case. In the present paper, such a general case is analyzed. The radical pair comes into contact in a mixed singlet-triplet state, and recombination proceeds from both the singlet and triplet states. The result obtained fully corresponds to the basic equation of spin chemistry.
Detection of photoreversibility of NO2–ONO linkage isomerization in [Co(NH3)5NO2]Cl(NO3 crystals by the photomechanical response method
Authors: Chizhik S. A. , Gribov P. A. , Kovalskii V. Yu. , Sidelnikov A. A.
Published: July 2024

Linkage isomerization NO2-ONO (nitro-nitrito) in the complex cation [Co(NH3)5NO2]2+ is a well-studied classical reaction. It is believed that the photoisomerization of the nitro form to nitrito in the crystalline phase achieves complete transformation at low temperature, while the reverse transformation proceeds as a first-order thermal intramolecular reaction upon heating of the crystals. To date, there is no information about the possibility of reverse photoisomerization. In this work, photoisomerization in [Co(NH3)5NO2]Cl(NO3) crystals is investigated by the analysis of crystal deformation caused by the transformation at different wavelengths. A change in the lattice parameters during the transformation leads to reliably measurable elongation and bending of acicular crystals. It is shown that the limiting elongation of the crystal under prolonged irradiation depends on the wavelength, which proves the reversibility of photoisomerization. The quantum yield of the reverse reaction is estimated to be 0.04 of the quantum yield of the direct reaction.
Kinetics of polaron capture by traps in a lithium niobate crystal
Authors: Fedorenko S.
Published: July 2024

The problem of reversible transformation and trapping of small-radius polarons in a lithium niobate crystal is considered within the framework of the integral encounter theory which is binary in the concentration of reatants. Analytical solutions are obtained for the relaxation kinetics of polarons, their lifetimes, and the rate constants of the corresponding channels of a multistage reaction controlled by polaron mobility. The temperature and concentration dependences of the observed quantities are analyzed. It is shown that at low temperatures polarons accumulate in a bound state characterized by an anomalously low relaxation rate.
Photochemistry of IrCl63– complex in aqueous solutions
Authors: Zhdankin G. I. , Grivin V. P. , Plyusnin V. F. , Tsentalovich Yu. P. , Glebov E. M.
Published: July 2024

The photochemistry of the [IrIIICl6]3- complex in aqueous solutions was studied by the methods of stationary and laser flash photolysis. As the result of a light quantum absorption, parallel processes of photoaquatation and photoionization occur. The aquated electron ea–q, which is formed with a quantum yield of 0.12 (excitation at 266 nm), is predominantly decayed in reactions with the initial complex and dissolved oxygen. The rate constant of ea–q capture by the [IrIIICl6]3- complex was measured. The main final photolysis products are Ir(III) complexes with different compositions of ligands, as well as several percents of Ir(IV) complexes. The formation of final products occurs in the time range from milliseconds to seconds.
Aerobic decomposition of dimethylthiourea nitrosyl iron complex in the presence of albimin and glutathione
Authors: Kormukhina A. Yu. , Kusyapkulova A. B. , Emel’yanova N. S. , Pokidova O. V. , Sanina N. A.
Published: July 2024

Nitrosyl iron complexes (NICs) are natural “depots” of NO. NICs forms by the interaction of endogenous nitric oxide (NO) and non‒heme [2Fe-2S] proteins. Their synthetic analogues are promising compounds in medicines for the treatment of socially significant diseases. In this paper, the effect of bovine serum albumin (BSA) and reduced glutathione (GSH) on the decomposition of a nitrosyl iron complex with N,N′-dimethylthiourea ligands [Fe(SC(NHCH3)2)2(NO)2]BF4 (complex 1) under aerobic conditions have been investigated. In the absorption spectra complex 1 in the presence of albumin a wide band at 370–410 nm appears, which indicates the coordination of the aerobic decay product of the complex in the hydrophobic pocket of the protein with Cys34 and His39. The quenching of albumin intrinsic fluorescence during titration with complex 1 was studied by fluorescence spectroscopy. The Stern-Vollmer constant K = (2.3 ± 0.2) ∙ 105 М-1 and the Förster radius 22.4 Å were calculated. The UV-spectrum complex 1 in presence of GSH has two peaks at 312 and 363 nm, which respond glutathione binuclear NICs.
Investigation of the behavior of dioxadet molecules in water by molecular dynamics simulation
Authors: Yakush E. A. , Kim A. V. , Medvedev N. N.
Published: July 2024

The behaviour of dioxadet molecules in water is studied by the molecular dynamics simulation. This substance has anti-cancer properties and is used in clinical practice. However, its properties have not yet studied at the molecular level. This paper presents the first attempt of such investigation. Parametrization of dioxadet molecule was carried out using different available services: ATB, SwissParam as well as AmberTools in a standard form and with the use of additional quantum-chemical calculations. The obtained models are compared with each other. The number of hydrogen bonds of the molecule with water was calculated, the analysis of hydrated water was carried out. It was shown that the dioxadet molecules in water tend to associate. All the models obtained show similar properties, but the quantitative characteristics differ noticeably. Further research is needed to select the best model. Molecule topology files are available for use.
Influence of different heat treatment regimes on the change of chemical composition and antibacterial activity of bee honey
Authors: Gruznov D. V. , Gruznova O. A. , Lobanov A. V. , Sokhlikov A. B. , Shcherbakova G. Sh. , Stepanova S. P. , Popov N. I.
Published: July 2024

The studies of the chemical composition and antibacterial activity of heather honey (Calluna vulgaris) subjected to heat treatment at 35–40°C for 12 hours were carried out. The temperature range (38–40°C), at which decrease in the H2O2 concentration, decrease in D-glucose-1-oxidase activity and increase in the 5-hydroxymethylfurfural content, was identified. The degree of chemical changes was directly proportional to the temperature and time of thermal exposure. The correlation between changes in the chemical composition and antibacterial activity of honey against test microorganisms Escherichia coli (strain 1257), Staphylococcus aureus (strain 209-P) and Bacillus cereus (strain 96) was established. The obtained results showed that heating honey to 37 °C even for 12 hours didn’t cause undesirable changes in its chemical composition and decrease in antibacterial activity. Thus, this temperature regime can be considered more gentle and recommended for use in the heat treatment of this food product.
Cationic effect in the formation of toxic and antiviral properties of Keggon heteropoly compounds
Authors: Dalidchik F. I. , Lopatina O. A. , Kovalevsky S. A. , Isaeva E. I. , Bidevkina M. V. , Baklanova O. V. , Gushchina E. A. , Lisitsyn F. V. , Balashov E. M. , Mezentseva M. V. , Pritchina T. N.
Published: July 2024

The Cytotoxicity indices (IC50) of Keggin’s phosphorus-molybdenum heteropoly acids (HPCAs) and their sodium and potassium salts on dog kidney cells (MDSC) were determined. The antiviral activity of these compounds against topical strains of influenza A (H3N2 and H1N1) was revealed. The dependence of the biological properties of polyoxometalates (POMs) on the elemental composition of their molecules has been confirmed. It has been shown that when some of the molybdenum atoms are replaced by vanadium atoms, HPCA and their salts acquire higher cytotoxicities, which increase monotonically as the number of substitutions increases. For the first time, the dependence of the biological activity of HPCA and their salts on the mass of cations has been established and interpreted. In vivo (on white outbred mice) the values of semi-lethal doses (DL50) of these compounds were established. For aqueous solutions of sodium and potassium salts of GPCA in a wide range of concentrations (from 0.05 μM to 15 μM), the values of the toxicity index (It) were determined on the model of motile cells. It has been established that GPCA and their salts are classified as moderately dangerous toxic substances and have selective antiviral activity, which at low concentrations (less than 15 μM) for influenza A strains is manifested mainly by a decrease in hemagglutination activity (HA).
Synthesis and characterization of prebiotic composite struvite/kappa-carrageenan
Authors: Kon’kova T. V. , Klushina N. V. , Romashchenko A. V. , Losev E. A. , Vedeeva A. D. , Sukhov B. G.
Published: July 2024

A struvite / kappa-carrageenan composite was synthesized from an aqueous solution of a polysaccharide and inorganic precursors. The phase, elemental composition and sizes of composite particles in an aqueous colloidal solution have been studied by X-ray phase analysis, X-ray energy dispersive analysis, and dynamic light scattering. It has been shown that this promising prebiotic composite is capable of producing diffusion-mobile aqueous colloidal solutions with varying degrees of association of structural elements, which is convenient for use in biomedicine.
CONSTRUCTING DIAGONAL LYAPUNOV–KRASOVSKII FUNCTIONALS FOR A CLASS OF POSITIVE DIFFERENTIAL-ALGEBRAIC SYSTEMS
Authors: Aleksandrov A. Yu.
Published: July 2024

A coupled system describing the interaction of a differential subsystem with nonlinearities of a sector type and a linear difference subsystem is considered. It is assumed that the system is positive. A diagonal Lyapunov–Krasovskii functional is constructed and conditions are determined under which the absolute stability of the investigated system can be proved with the aid of such a functional. In the case of nolinearities of the power form, estimates for the convergence rate of solution to the origin are derived. The stability analysis of the corresponding system with parameter switching is fulfiled. Sufficient conditions guaranteeing the asymptotic stability of the zero solution for any admissible switching law are obtained.
METHOD FOR CONSTRUCTING PERIODIC SOLUTIONS OF NONLINEAR DIFFERENTIAL EQUATIONS
Authors: Budanov V. M.
Published: July 2024

A justification is given for the analytical method for constructing periodic solutions for nonlinear systems of ordinary differential equations of polynomial type. Periodic solutions are constructed in the form of Fourier series, in which the coefficients are polynomials depending on the parameter without the assumption of its smallness. Two examples are considered — the van der Pol equation and the Lorentz system.
THE EXISTENCE AND UNIQUENESS THEOREMS FOR STOCHASTIC DIFFERENTIAL-DIFFERENCE HYBRID SYSTEMS
Authors: Levakov A. A. , Dolzhenkova D. A.
Published: July 2024

A stochastic differential-difference hybrid system is a system of interacting variables whose dynamics are described by stochastic differential equations for some of them and difference equations for others. Systems with two types of difference equations are examined: first, a difference equation in the form of a process involving the multiplicative Wiener process, and second, a difference equation with delay. The existence and uniqueness theorems for both systems have been proven. The basic conditions on the system’s parameters are local Lipschitz conditions and linear growth order.
TYPICAL DROPPING ASYMPTOTICS OF QUASICLASSICAL APPROXIMATIONS TO SOLUTIONS OF THE NONLINEAR SCHRÖDINGER EQUATION
Authors: Melikhov S. N. , Suleimanov B. I. , Shavlukov A. M.
Published: July 2024

Formal asymptotics are substantiated that describe typical dropping cusp singularity of quasiclassical approximations to solutions of two cases of the integrable nonlinear Schr¨odinger equation −𝑖𝜀Ψ′𝑡=𝜀2Ψ′′𝑥𝑥±2|Ψ|2Ψ, where 𝜀 is a small parameter. The substantiation uses the ideology and facts of the mathematical catastrophe theory and the part of the theorem of Yu.F. Korobeinik, concerning analytical as ℎ → 0 solutions 𝐺(ℎ, 𝑢) of the mixed type linear equation ℎ𝐺′′ℎℎ = 𝐺′′𝑢𝑢 to which the hodograph images of both cases of the systems of equations of these quasiclassical approximations are equivalent.
ABOUT THE CORE STRUCTURE OF THE SCHWARTZ PROBLEM FOR FIRST-ORDER ELLIPTIC SYSTEMS ON A PLANE
Authors: Nikolaev V. G.
Published: July 2024

The Schwarz problem for 𝐽-analytic functions in an arbitrary ellipse is considered. The matrix 𝐽 is assumed to be two-dimensional, with different eigenvalues lying above the real axis. An example of a nonconstant solution of the homogeneous Schwarz problem in the form of a vector-polynomial of degree three is given. The numerical parameter 𝑙 of the matrix 𝐽, which is expressed through its eigenvectors, is introduced. After that, one relation derived earlier by the author is analyzed. Based on this analysis, a method for computing the dimension and structure of the kernel of the Schwarz problem in an arbitrary ellipse is obtained. Sufficient conditions for the triviality of the kernel expressed through the ellipse parameters, the eigenvalues of the matrix 𝐽 and the parameter 𝑙 are obtained. Examples of one-dimensional and trivial kernels are given.
MULTIPLICATIVE CONTROL PROBLEMS FOR THE DIFFUSION-DRIFT CHARGING MODEL OF AN INHOMOGENEOUS POLAR DIELECTRIC
Authors: Brizitskii R. V. , Maksimova N. N.
Published: July 2024

A two-parameter multiplicative control problem is studied for a model of electron-induced charging of an inhomogeneous polar dielectric. Exact estimates of the local stability of its optimal solutions with respect to small perturbations of both the cost functionals and the given function of the boundary value problem are derived. For one of the controls, the relay property or the bang-bang principle is established.
BACKSTEPPING STABILIZATION OF NONLINEAR DYNAMICAL SYSTEMS UNDER STATE CONSTRAINTS
Authors: Golubev A. E.
Published: July 2024

The problem of stabilizing the zero value of the state vector of constrained nonlinear dynamical systems written in a special form is solved. The proposed control design accounts for magnitude constraints on the values of state variables and is based on the integrator backstepping approach using logarithmic Lyapunov barrier functions. The obtained stabilizing feedbacks, in contrast to similar known results, are based on the use of linear virtual stabilizing functions that do not grow unbounded as state variables approach the boundary values. As an example, we consider a state constraints aware solution to the control problem of positioning an autonomous underwater vehicle at a given point in space.
APPROXIMATION OF FUNCTIONAL-ALGEBRAIC EIGENVALUE PROBLEMS
Authors: Korosteleva D. M.
Published: July 2024

A new symmetric variational functional-algebraic formulation of the eigenvalue problem in the Hilbert space with linear dependence on the spectral parameter for a class of mathematical models of thin-walled structures with an attached oscillator is proposed. The existence of eigenvalues and eigenvectors is established. A new symmetric approximation of the problem in a finite-dimensional subspace with linear dependence on the spectral parameter is constructed. Error estimates of the approximate eigenvalues and eigenvectors are obtained. The theoretical results are illustrated on the example of the problem of structural mechanics.
Antibiofilm and Probiofilm Effects of Nanomaterials on Microorganisms
Authors: Maksimova Yu. G. , Zorina A. S.
Published: July 2024

The review summarizes and analyzes information regarding the effect of nanoparticles (NPs) of metals, metal oxides and carbon on the biofilm formation and mature biofilms of microorganisms. The viability of individual microbial cells, including direct disruption of cell surface structures and oxidative stress associated with the formation of reactive oxygen species (ROS), as well as the effect on the production of the exopolymer matrix and the quorum sensing system are considered as the mechanisms of NPs action on biofilms. The effects of silver NPs, gold NPs, some metal oxides, and carbon nanomaterials on microbial biofilms have been described in more detail. The effects of metal and carbon NPs on microbial biofilms are compared. Both antibiofilm and probiofilm effects of NPs are noted, depending on their nature, and the prospect of their use as antimicrobial agents and carriers for the production of microbial biofilms of biotechnological significance are considered.
Application of Detectr for Selective Detection of Bacterial Phytopathogen Dickeya solani Using Recombinant CRISPR-Nuclease Cas12a Obtained by Single-Stage Chromatographic Purification
Authors: Kurbatov L. K. , Radko S. P. , Khmeleva S. A. , Ptitsyn K. G. , Timoshenko O. S. , Lisitsa A. V.
Published: July 2024

The work demonstrates that recombinant CRISPR-nuclease Cas12a, purified after heterologous expression with a simplified method using single-stage metal-chelate chromatography, can be successfully utilized in DETECTR technology. The combination of CRISPR-nuclease Cas12a obtained by such way with recombinase polymerase amplification (RPA) allowed one to ensure the selectivity of detection of Dickeya solani — the dangerous bacterial phytopathogen causing the potato disease known as “blackleg” — against closely related and unrelated bacterial phytopathogens with a limit of detection of 1 copy of the bacterial genome per amplification reaction. The result can be determined visually, without the use of complex instrumental methods, by changing the color of the reaction sample when illuminated with blue light that creates the basis for development of field DNA diagnostics of D. solani. The use of simplified chromatographic purification will significantly reduce the time and resources required to obtain a functionally active CRISPR-nuclease Cas12a for development and production of DNA diagnostics based on DETECTR technology.
Comparison of the Efficiency of Various Promoters for the Production of Secreted β-Mannanase by Bacillus subtilis by Cells of the Methylotrophic Yeast Ogataea haglerorum
Authors: Podpletnev D. A. , Lapteva A. R. , Sineoky S. P. , Tarutina M. G.
Published: July 2024

In this article, strong promoters of thermotolerant methylotrophic yeast Ogataea haglerorum have been characterized. Promoters play a key role in the regulation of gene expression; therefore, they are the important element of expression vectors. Strong and strictly regulated promoters are a powerful tool for creating highly productive strains — producers of recombinant proteins. To expand the potential of the O. haglerorum expression system natural methanol-induced promoters of the OhMOX and OhFMD genes and the constitutive promoter of the OhGAP gene were studied in comparison with the promoter of the MOX gene from O. polymorpha yeast. A gene encoding recombinant β-mannanase was used as a reporter gene. It has been shown that in O. haglerorum yeast cells, the expression level (strength) of the pOhMOX promoter is about 1.4–1.9 times higher relative to the pOpMOX promoter from O. polymorpha yeast. The obtained data on the strength of promoters from yeast O. haglerorum can be useful in designing producers of recombinant proteins and optimizing metabolic pathways in methylotrophic yeast O. haglerorum.
Oxidative Damage and Antioxidant Response of Acinetobacter calcoaceticus, Pseudomonas putida and Rhodococcus erythropolis Bacteria during Antibiotic Treatment
Authors: Sazykin I. S. , Plotnikov A. A. , Lanovaya O. D. , Onasenko K. A. , Polinichenko A. E. , Mezga A. S. , Azhogina T. N. , Litsevich A. R. , Sazykina M. A.
Published: July 2024

In this work, oxidative damage and the level of antioxidant response in Acinetobacter calcoaceticus, Pseudomonas putida, and Rhodococcus erythropolis cells under the influence of such antibiotics as ampicillin, azithromycin, rifampicin, tetracycline, and ceftriaxone were studied. The level of protein carboxylation and lipid peroxidation (LPO), as well as the activity of superoxide dismutase (SOD), catalase, glutathione reductase (GR), and the level of glutathione 3 and 6 hours after antibiotic treatment of bacteria were assessed. It is observed that SOD induction occurs earlier and is more active than catalase induction. In A. calcoaceticus, SOD is induced together with protein carboxylation and probably protects them from oxidative damage, while catalase induction correlates with LPO. A positive correlation is also noted between catalase activity and glutathione content in R. erythropolis. Catalase activity increases insignificantly and even decreases under the studied antibiotics influence, which is associated with an insignificant level of lipid peroxidation in most prokaryotes. On the other hand, low catalase activity can contribute to genome destabilization as a result of oxidative stress and enhance the adaptive evolution of bacteria.
Metabolic Potential of Serratia sp. 22S for Chlorpheoxyacetic Acids Conversion
Authors: Zharikova N. V. , Zhurenko E. I. , Korobov V. V. , Anisimova L. G. , Aktuganov G. E.
Published: July 2024

A bacterial strain 22S belonging to the genus Serratia was isolated from soil samples contaminated with chemical production wastes. The strain was found to be non-pathogenic based on the study of its virulence, toxicity, infectivity and invasiveness. In batch culture, Serratia sp. 22S was able to separately utilize chlorophenoxyacetic acids (100 mg/L) as the sole source of carbon and energy. The catabolism pathway for chlorophenoxyacetic acids were suggested through complete reductive dechlorination of the substrate followed by meta-cleavage of the aromatic ring of catechol based on the compounds found in the culture medium (2,4-dichloro-6-methylphenoxyacetic, phenoxyacetic, and 2-hydroxy-2-hexenedioic acids). Intact cells experiments confirmed this assumption. In model systems, good adaptability and survival of the 22S strain in the soil was revealed, and the content of chlorophenoxyacetic acids up to a certain concentrations had a positive effect on the growth of the strain, most likely due to its selective effect.
Properties of Extracellular Protease — Regulator of Hemostasis Produced by Micromycete Aspergillus tabacinus
Authors: Lavrenova V. N. , Kreyer V. G. , Savkovic Z. , Osmolovskiy A. A.
Published: July 2024

The extracellular protease with protein C-like and plasmin-like activities was isolated from the culture fluid of the micromycete A. tabacinus BEOFB3260m. It has been established that A. tabacinus extracellular protease is a non-glycosylated serine protease with mol. weight about 30 kDa. The enzyme is active and stable at temperature of 25–37°, active at pH 7.0–12.0 and stable at pH 3.0–12.0 and is a perspective candidate for new anticoagulant drugs development.
Evaluation of the Content of Steroid Phytohormones in Humic Preparations
Authors: Litvinovskaya R. P. , Sauchuk A. L. , Denisiuk D. V. , Perakhod D. G. , Perakhod R. V. , Khripach V. A.
Published: July 2024

It was shown for the first time that humic preparations contain steroidal phytohormones — brassinosteroids (BS), the amount and composition of which vary depending on the source of raw materials and the method of its processing. It has been established that the qualitative and quantitative composition of BS correlates with the content of humic substances (humic acids). Using the example of humic preparations obtained from sapropel, it was found that alkaline treatment leads to the release of a noticeable amount of BS present in the form of conjugates. These results indicate that BS are important components of humic preparations and undoubtedly contribute to a wide range of physiological effects of these agricultural preparations.
Nutritional and Possible Medicinal Value of Siberian Sturgeon (Acipenser baerii) and Sterlet (Acipenser ruthenus) Ovarian Fluid
Authors: Mikhailova M. V. , Zolotarev K. V. , Mikhailov A. N. , Nakhod V. I. , Zgoda V. G. , Kharenko E. N.
Published: July 2024

Chemical analysis of samples of ovarian fluid (OF), a secondary product of breeding of the two common in Russian aquaculture species of Acipenseridae family, was carried out in order to assess its nutritional and possible medicinal value. It has been figured out that the dominant organic component of OF of both species is a water-soluble or water-dispersible protein easily absorbed by the digestive tract. Proteomic analysis of the samples has shown that the main component of the protein fraction of OF is a highly nutritious protein vitellogenin. Amino acid analysis has shown that OF samples of both species satisfy the needs of an adult human in all essential amino acids. Eighty-five grams of dried sturgeon OF or 55 g of dried sterlet OF covers the daily requirement of an adult for vitamins C, B1, B2, B3 (PP) and B6, a number of macronutrients (Na, K, Ca, Mg) and micronutrients (Fe, Cu, Mn, Zn, Cr). Furthermore, Cu content is significantly higher in sturgeon OF, and Fe content is significantly higher in sterlet OF, which is partly confirmed by the relative content of Cu and Fe carrier proteins, ceruloplasmin and transferrin respectively. In addition, a number of proteins of physiological significance for fish were identified. The possible medicinal value of OF consists, among other things, in significant content of various antioxidant proteins in OF of both species. The use of OF as a food supplement could improve the profitability of sturgeon breeding by producing an additional valuable product, and would also expand the range of natural dietary supplements on the market.
Modeling seawater intrusion upon water withdrawal from coastal aquifers
Authors: Drobyazko E. V. , Rastorguev A. V.
Published: July 2024

Seawater intrusions into coastal aquifers have been the object of study by hydrogeologists since long ago. A wide range of methods are used to predict the development of intrusions: analytical, numerical analytical and numerical. The purpose of this paper is to identify and evaluate seawater intrusion using a coastal groundwater intake as an example. The paper considers a coastal groundwater intake, where mineralization, total hardness and chlorides have increased due to intensive exploitation. Seawater intrusion is assumed to be the main reason for the groundwater quality deterioration. To prove the seawater intrusion motion, chemical sampling of water from the intake wells was carried out, which resulted in chlorine-bromine coefficient values indicating the presence of seawater admixture in fresh groundwater. The seawater intrusion was evaluated using numerical-analytical and numerical modelling. The estimation obtained by the analytical element method (GFLOW code) indicates the presence of seawater intrusion at the studied water intake. However, the analytical element method calculations provide the ultimate steady-state estimate of the intrusion development. Also, the method uses the assumption of a sharp freshwater-saltwater boundary. Numerical modelling provides a more realistic assessment. In building the three-dimensional numerical model, special attention was paid to the nature of the connection between the exploited aquifer and the sea. In the onshore part, the Quaternary aquifer is separated from the exploited aquifer by a clay layer with interbedded gravel and sand, but there is no data on the composition of sediments beneath the sea. To clarify their composition, profile numerical models were built in the FEFLOW program with different degrees of connection between the sea and the exploited aquifer. One of the models suggests a direct connection between the sea and groundwater through sand, while the other one is complicated by clay. According to the results of the profile models, the direct connection between the exploited aquifer and the sea was proved, which was taken into account in the construction of the three-dimensional numerical model. On the numerical model in the SEAWAT program, the change in the seawater intrusion position in different periods of water intake operation with different water withdrawal values was obtained, after which the calculation results according to numerical-analytical and numerical models were compared.
Modeling the leaching of aluminophosphate glass in the presence of bentonite
Authors: Boldyrev K. A.
Published: July 2024

The article presents a model of aluminophosphate glass leaching, an analogue of the RW glass matrix, in static mode in the presence of bentonite. Parameterization of the model is based on experimental data. The model takes into account the kinetics of the glass matrix leaching and the transformation of bentonite mineral phases; sorption processes are also taken into account, as well as the inhibition of leaching by the precipitation of secondary mineral phases formed. The model demonstrates a small transformation of clay phases, as well as the formation of secondary phosphate phases and gibbsite upon leaching of glass in the contact zone with bentonite. The simulation was carried out in the PhreeqC software.
New technology of protecting a stationary engineering structure from landslide pressure
Authors: Postoev G. P. , Kazeev A. I. , Kuchukov M. M. , Orlova N. A.
Published: July 2024

The methodology of protecting stationary objects (foundations of high-voltage network, cable road, overpasses, etc.) located on an active landslide slope is considered. A new approach to implementation of protective measures is proposed. It is based on the identified features of formation of new geological structures in the ground massif at the local area of technogenic impact. Dissipative geological structures develop in the landslide massif upon its thrusting on a immobile object. In particular, a core and boundary envelopes are formed at the contact with the frontal surface of the object, in accordance with the parameters of the latter. A methodology is proposed to ensure the flow of landslide masses around the object and its stability.
Zoning of the north-eastern part of Ufa according to the development of hazardous geological processes
Authors: Epifanova D. R. , Travkin A. I. , Krinochkina O. K.
Published: July 2024

The article is focused on consideration of the engineering geological conditions of the area situated in the north-eastern residential part of the Ufa city (the capital of Bashkortostan). The majority of urban population lives in the Ufa-Belsko-Shugurovsky interfluve area. Exogenous hazardous geological processes are extensively developed there. The most wide-spread are karst, karst-suffusion, slope and erosion, as well as other engineering geological processes, which are manifested in the relief by sinkholes, craters, landslides, ravines and washouts. The research was based on a set of schematic maps compiled in 2013 according to archive data for the development of the Ufa master plan on a scale of 1:10000, as well as the updated versions of these maps [3]. The maps were created considering the state of the most problematic areas of geohazard activation. The occurrence of these processes was previously investigated by satellite and aerial images, and also in field by route survey as a part of reconnaissance. Archival geological information from the mining documentation was also used when compiling the maps. Engineering geological research and zoning territories with manifestation of karst, karst-suffusion, slope and other hazardous geological processes is proved to be relevant for the design of buildings and engineering structures. Currently, the engineering geological background data on the Ufa city territory is quite plentiful, and yet it remains uneven in the study area.
Bottom ash from municipal solid waste incineration: composition, leaching of heavy metals, treatment, possibilities of application and permissibility of burial
Authors: Yuganova T. I. , Putilina V. S.
Published: July 2024

The most important problem that arises when handling bottom ash from municipal solid waste incineration, due to possible environmental pollution, is considered. The composition, chemical properties and mineralogy of bottom ash are presented. Issues of pollutant leaching from bottom ash, leaching testing methods, and predictive modelling are considered. Heavy metals as the most hazardous pollutants for the environment have been studied. The role of pH and dissolved organic carbon in leaching heavy metals is described. Methods for processing bottom ash to reduce environmental impact, the possibilities of using slag and regulatory restrictions are presented.
On the reclamation of land disturbed by mining in Kuzbass using applied geophysical technologies
Authors: Solovitskii A. N. , Nikulin N. Yu.
Published: July 2024

The experience in reclamation of disturbed lands in Kuzbass is summarized. The demand for studying the spatial distribution of soil composition and water content in soil at the technical stage of land reclamation is shown. Currently, there is a need to develop new methods for the reclamation of disturbed lands, aimed at ensuring further development of depleted areas. The goal of research is to develop a method for reclamation of lands disturbed by mining operations in Kuzbass, based on the use of applied geophysical technologies by the example of the Taldinsky coal mine. The proposed method is based on the zoning of the mined-out area proceeding from the results of applied shallow geophysical survey on the state of soils and their water content, which provides a new level of information interaction between the technical and biological stages of reclamation. The practical use of this method is to expand the possibilities of reclamation of disturbed lands for the development of rational environmental management.
PENGARUH KUALITAS PELAYANAN TERHADAP KEPUASAN PASIEN PADA RUMAH SAKIT ELANG MEDIKA CORPORA (EMC) CIKARANG
Authors: Dani Sanjani Aji Gunawan , Hermana Cecep
Published: July 2024

Rumah Sakit EMC Cikarang merupakan rumah sakit yang memiliki pelayanan terbaik kelas B. RS EMC Cikarang dibuka untuk umum sejak 2016 dibawah PT. Elang Medika Corpora. RS EMC Cikarang menyediakan 5 pelayanan unggulan yaitu spesialis anak, spesialis tulang, kebidanan & penyakit kandungan, penyakit dalam. Tujuan penelitian ini untuk mengukur tingkat kualitas pelayanan RS EMC Cikarang yang sangat berpengaruh terhadap kepuasan konsumen.  Sehingga, keberlangsungan hidup perusahaan akan bertahan dalam jangka panjang jika kualitas pelayanan yang diciptakan dapat menimbulkan kepuasan konsumen. Metode penelitian ini dilakukan dengan cara memberikan lembaran-lembaran kertas yang berisi qr code yang telah dibuat mengarah ke google formulir dan lembaran kuisioner tulis tangan untuk mengisi kuisioner yang berisi 33 pertanyaan dari 2 variabel. Sehingga didapatkan 59 sampel responden yang telah mengisi kuisioner, terbagi menjadi 2 yaitu 34 didapatkan dari hasil kuisioner qr code google formulir dan 25 didapatkan dari hasil kuisioner lembar kertas tulis tangan. Data kuisioner ini diolah menggunakan analisis regression linear yang menghubungkan antara variabel independent (kualitas pelayanan) dan variabel dependent (kepuasan konsumen) menggunakan softaware statistical product and service solutions 16.0. Hasil dari penelitian ini yang diambil dari 59 responden rawat jalan dan rawat inap di RS EMC Cikarang yang menunjukan bahwa ada 2 dari 5 variabel independent (jaminan dan empati) yang mempengaruhi variabel dependent (kepuasan konsumen), 3 dari variabel independent (Reliabilitas, Daya Tanggap, dan Bukti Fisik) tidak berpengaruh terhadap variabel dependent (kepuasan konsumen). Maka, sangatlah penting bagi rumah sakit yang merupakan suatu perusahaan pelayanan produk jasa untuk menganalisa dan menciptakan kualitas pelayanan demi membentuk kepuasan konsumen.
EVOLUSI ISU EARNINGS MANAGEMENT DALAM LITERATUR BISNIS : ANALISIS BIBLIOMETRIK
Authors: Krisnanda Karina
Published: July 2024

Penelitian ini menggunakan analisis bibliometrik untuk menelusuri perkembangan dan perubahan dalam penelitian earnings management atau manajemen laba dari tahun 2005 hingga 2023. Dengan menganalisis 1.315 artikel yang berfokus pada bidang bisnis, manajemen, dan akuntansi, penelitian ini menemukan peningkatan signifikan dalam jumlah publikasi terkait manajemen laba, yang kemungkinan didorong oleh meningkatnya perhatian terhadap skandal akuntansi. Analisis ini juga mengungkap tren topik yang berfokus pada investasi dan penilaian risiko. Penelitian ini memberikan kontribusi pada literatur manajemen laba dengan mengidentifikasi area penelitian utama dan menyediakan landasan teoretis untuk penelitian selanjutnya.
KONTRIBUSI STRATEGI PROMOSI TERHADAP KEPUTUSAN PEMBELIAN PRODUK KOSMETIK HALAL
Authors: Oktafani Farah , Nidar Sulaeman Rahman
Published: July 2024

Indonesia merupakan salah satu negara mayoritas muslim terbesar di dunia, dengan tingginya angka konsumsi barang pada konsumen muslim menjadikan peluang mengembangkan produk halal sangat terbuka lebar demi memenuhi kebutuhan pasar. Penggunaan unsur Syariah dalam pemasaran bertujuan untuk menghindari pelanggaran etika yang berdampak negatif pada citra, persepsi, niat beli produk kosmetik halal. Penggunaan unsur Syariah dalam pemasaran bertujuan untuk menegaskan bahwa etika promosi yang diberikan oleh Islam bersifat universal. Penelitian ini bertujuan menganalisis pengaruh promosi terhadap keputusan pembelian konsumen pada produk kosmetik halal. Metode yang digunakan adalah kuantitatif dengan teknik analisis regresi linear berganda. Populasi yang digunakan adalah konsumen produk kosmetik halal dengan sampel sebanyak 100 responden. Responden penelitian didominasi oleh wanita dewasa dengan pekerjaan sebagai pegawai swasta yang sudah memiliki persepsi kuat mengenai suatu produk tanpa harus dipengaruhi stimulus periklanan, publisitas, promosi penjualan ataupun pemasaran langsung. Hasil penelitian menunjukan bahwa strategi promosi berpengaruh terhadap keputusan pembelian kosmetik halal secara simultan, tetapi secara parsial hanya penjualan personal yang mempengaruhi keputusan pembelian.