bluuebunny/crossref_metadata_2025 huggingface dataset:
Indicators of Quality of Care in Individuals With Traumatic Spinal Cord Injury: A Scoping Review
Authors: Khosravi Sepehr , Khayyamfar Amirmahdi , Shemshadi Milad , Koltapeh Masoud Pourghahramani , Sadeghi-Naini Mohsen , Ghodsi Zahra , Shokraneh Farhad , Bardsiri Mohadeseh Sarbaz , Derakhshan Pegah , Komlakh Khalil , Vaccaro Alex R. , Fehlings Michael G. , Guest James D. , Noonan Vanessa , Rahimi-Movaghar Vafa
Published: January 2021

Study Design:

Scoping review.

Objectives:

To identify a practical and reproducible approach to organize Quality of Care Indicators (QoCI) in individuals with traumatic spinal cord injury (TSCI).

Methods:

A comprehensive literature review was conducted in the Cochrane Central Register of Controlled Trials (CENTRAL) (Date: May 2018), MEDLINE (1946 to May 2018), and EMBASE (1974 to May 2018). Two independent reviewers screened 6092 records and included 262 full texts, among which 60 studies were included for qualitative analysis. We included studies, with no language restriction, containing at least 1 quality of care indicator for individuals with traumatic spinal cord injury. Each potential indicator was evaluated in an online, focused group discussion to define its categorization (healthcare system structure, medical process, and individuals with Traumatic Spinal Cord Injury related outcomes), definition, survey options, and scale.

Results:

A total of 87 indicators were identified from 60 studies screened using our eligibility criteria. We defined each indicator. Out of 87 indicators, 37 appraised the healthcare system structure, 30 evaluated medical processes, and 20 included individuals with TSCI related outcomes. The healthcare system structure included the impact of the cost of hospitalization and rehabilitation, as well as staff and patient perception of treatment. The medical processes included targeting physical activities for improvement of health-related outcomes and complications. Changes in motor score, functional independence, and readmission rates were reported as individuals with TSCI-related outcomes indicators.

Conclusion:

Indicators of quality of care in the management of individuals with TSCI are important for health policy strategists to standardize healthcare assessment, for clinicians to improve care, and for data collection efforts including registries.
Hand, Wrist, and Forearm Injuries in Male Professional Soccer Players: A Prospective Cohort Study of 558 Team-Seasons From 2001-2002 to 2018-2019
Authors: Andersson Jonny K. , Bengtsson Håkan , Waldén Markus , Karlsson Jón , Ekstrand Jan
Published: January 2021

Background:

The literature on upper extremity injuries in professional soccer players is scarce, and further insight into the onset and cause of these injuries as well as potential differences between goalkeepers and outfield players is important.

Purpose:

To investigate the epidemiology of hand, wrist, and forearm injuries in male professional soccer players between 2001 and 2019.

Study Design:

Descriptive epidemiology study.

Methods:

Between the 2001-2002 and 2018-2019 seasons, 120 European male soccer teams were followed prospectively for a varying number of seasons (558 team-seasons in total). Time-loss injuries and player-exposures to training sessions and matches were recorded on an individual basis in 6754 unique players. Injury incidence was reported as the number of injuries per 1000 player-hours, and between-group differences were analyzed using Z statistics and rate ratios (RRs) with 95% CIs. Between-group differences in layoff time were analyzed.

Results:

In total, 25,462 injuries were recorded, with 238 (0.9%) of these affecting the hand (71.4%; n = 170), wrist (16.8%; n = 40), and forearm (11.8%; n = 28), producing an incidence of 0.065 injuries per 1000 hours. A majority of the injuries were traumatic with an acute onset (98.7%; n = 235). Fractures were the most common injuries recorded (58.8%; n = 140), often involving the metacarpal bones (25.2%; n = 60) and phalanges (10.1%; n = 24). The injury incidence was significantly higher for goalkeepers (115 injuries; 0.265 per 1000 hours) compared with outfield players (123 injuries; 0.038 per 1000 hours) (RR, 7.0 [95% CI, 5.4-9.0]). Goalkeepers also had a significantly longer mean layoff time than outfield players (23 ± 27 vs 15 ± 27 days; P = .016).

Conclusion:

Injuries to the hand, wrist, and forearm constituted less than 1% of all time-loss injuries in male professional soccer players. Fractures were most common and constituted more than half of all injuries. Goalkeepers had a 7-fold higher incidence and an over 1-week longer mean layoff time compared with outfield players.
Nutrition Standards For Infants and Young Children can be Implemented by Family Child Care Home Providers
Authors: Ritchie Lorrene D. , Keeton Victoria , Lee Danielle L. , Gurzo Klara , Homel Vitale Elyse , Au Lauren E. , Alkon Abbey
Published: January 2021

The study evaluated an educational intervention with family child care home (FCCH) providers to implement nutrition standards. A convenience sample of licensed California FCCH providers (n = 30) attended a 2-hour, in-person group training in English or Spanish on nutrition standards for infants and children aged 1 to 5 years. Provider surveys and researcher observations during meals/snacks were conducted pre- and 3 months post-intervention. Providers rated the training as excellent (average score of 4.9 on a scale of 1-5). Adherence, assessed by survey and observation and compared over time using paired t-tests, increased from an average of 36% pre-intervention to 44% post-intervention ( P = .06) of providers (n = 12) for infant standards and from 59% to 68% ( P < .001) of providers (n = 30) for child standards. One-third (39%) of providers rated infant standards and 19% of providers rated child standards as difficult to implement. Nutrition standards can be implemented by FCCH providers after an educational intervention; a larger study is warranted with a representative group of providers.
The Two-bag System for Intravenous Fluid Management of Children with Diabetic Ketoacidosis: Experience from a Community-Based Hospital
Authors: Hasan Rashed A. , Hamid Kewan , Dubre Donald , nolan brian , Sharman Mahesh
Published: January 2021

Objectives: Intravenous fluid (IVF) administration using the two-bag system compared with the one-bag system in children with diabetic ketoacidosis (DKA) admitted between January 1, 2015 and December 31, 2016. Design: Retrospective cohort study. Setting: Community-based hospital. Results: A total of 109 patients were enrolled with a mean age of 13.24 years. The 2 groups had comparable demographics. Initial laboratory results were similar except for initial PH and Sodium. The two bag system had significantly less number of calls compared to one bag system (25.2 vs 5.2 P = .0001). One bag system had fewer hypoglycemia <60 mg/dl (4 vs 12 P = .049). No statistically significant observations noted in regards to glucose drop rate, number of intravenous fluid bags used, amount of fluid boluses given, hospital stay and Pediatric ICU stay. Conclusions: The two-bag system has less resource utilization and slower blood glucose drop rate, but higher hypoglycemic events
A data-driven complex network approach for planning sustainable and inclusive urban mobility hubs and services
Authors: Tran Martino , Draeger Christina
Published: January 2021

New mobility services that facilitate multimodal options are important for strategic urban transport systems planning. Part of this strategy is municipal investment in urban mobility hubs to increase access to mobility services. We present a new evaluation framework and algorthim to locate and assess the sustainability and equity impacts of hubs in cities. Scenarios are used to evaluate hub investment strategies in different cities that prioritize (1) current mode split, (2) high transit capacity, and (3) multimodal services. From an equity perspective, high transit capacity and multimodal hub strategies include more low-income areas than current mode split, which covers middle-income areas most. Travel times to access the nearest hub in Portland by low-income households is ∼20–40 min compared to high-income households requiring ∼25–30 min. Seattle and Vancouver perform better requiring ∼15–20 min for low-income compared to ∼25–35 min for high-income households. Multimodal hubs are the most efficient requiring ∼15–20 minutes to reach compared to ∼15–30 minutes for high capacity and current mode split scenarios. From a sustainability perspective, ∼10%–50% of the population cannot reach a hub within 30 minutes by public transit compared to <10% by car, and travel time to reach the nearest hub in all three cities by car is <20 min compared to ∼20–40 min by public transit. Between all cities, low-income households representing ∼2%–15% of the total population have no access to a hub by public transit within 30 min compared to high-income households representing ∼1%–3% of the total population. Only in Portland are there low-income households not able to reach a hub by car, and in each city, all high-income households can reach at least one hub by car within 30 min. Our results show how municipalities can strategically invest in public transit and multimodal options to increase the frequency, quality, and overall mobility for low- and medium-income households and improve access to essential amenities for more vulnerable citizens. Municipalities can use our hub evaluation framework to explore alternative transport investment scenarios and spatially locate urban hubs to meet future travel demand, increase adoption of multimodal services, and improve equitable access for all citizens.
Arthrodesis of Ipsilateral Hallux Metatarsophalangeal and Interphalangeal Joints
Authors: Brodsky James W. , Zide Jacob R. , Kim Kim Eung Soo , Charlick Daniel A. , Daoud Yahya , Bohl Daniel D.
Published: January 2021

Background:

Arthrodesis of the ipsilateral hallux metatarsophalangeal (MTP) and interphalangeal (IP) joints may be required for severe arthritis or deformity at both joints. The purpose of this study was to review outcomes of ipsilateral first MTP and IP joint arthrodesis.

Methods:

Twenty feet were identified, for which the diagnosis was rheumatoid arthritis in 14, failed hallux valgus surgery in 5, and hallux rigidus in 1. The IP arthrodesis was performed first in 6 feet; MTP first in 8 feet; and both joints simultaneously in 6 feet. Median follow-up was 28 months (range 12-94). Medical records and radiographs were reviewed. American Orthopaedic Foot & Ankle Society (AOFAS) score and patient satisfaction were determined.

Results:

Although all of the MTP arthrodeses healed, 8 of 20 feet (40%) failed to heal at the IP arthrodesis. The rate of IP nonunion was 17% (1/6) with IP arthrodesis first, 50% (4/8) with MTP arthrodesis first, and 50% (3/6) with simultaneous arthrodesis. Four of 8 IP nonunions were symptomatic. Subsequent surgery was required in 11 feet (55%), including repair of IP nonunion in 3 feet, hardware removal in 4, revision MTP malunion in 2, wound debridement in 1, and soft tissue reconstruction in 1. Median hallux AOFAS score for the cohort increased from 25 to 68. Eighteen feet resulted in patients who were very satisfied or satisfied with minor reservations. Neither AOFAS score nor satisfaction trended toward association with IP union.

Conclusion:

Ipsilateral arthrodesis of the hallux MTP and IP joints was challenging because of high rates of reoperation and IP nonunion, the latter of which was likely related to increased mechanical stress on the IP joint with immobilization of the MTP joint. Despite the high IP nonunion rate, IP nonunion did not predict patient-reported outcome. Fibrous ankylosis was an acceptable clinical outcome in many cases.

Level of Evidence:

Level IV, case series.
A Study of Fiber Laser Micro-Grooving of 316L Stainless Steel at Different Temperatures
Authors: Sen A. , Doloi B. , Bhattacharyya B.
Published: January 2021

The present article deals with the generation of micro-grooves on 316L stainless steel (SS) by a nanosecond pulsed fiber laser system. Fabrication of micro-grooves on 316L SS has immensely contributed to the biomedical and automotive industries through improving the mechanical, lubrication, and corrosion resistance properties. In the present work, the considered process parameters are the preheating temperature (100°C and 200°C), along with the room temperature (24°C), cutting speed, sawing angle, pulse frequency, and laser power. The ranges of cutting speed and sawing angle are 0.1–1.1 mm s−1 and 0.1°–1°, respectively. Besides, pulse frequency and laser power vary from 55 kHz to 85 kHz and from 15 W to 45 W, respectively. The constant parameters are the pulse width of 99% and assist air pressure of 6 kgf cm−2. The variable parameters for the analysis are cut width and heat-affected zone (HAZ) width. The article aims to showcase a comprehensive study of fiber laser process parameters at different temperatures (preheated condition and room temperature) with variable sawing angles to produce better process control and bring about each considered process parameter’s critical value. The experimental results show that higher dimensions of cut width and HAZ width are observed at 200°C with the increment of sawing angle and laser power, compared to other temperatures. With the increment of cutting speed and laser power, the HAZ width tends to rise sharply. A significant drop in cut width and HAZ width dimensions is observed with the increment in pulse frequency for any temperature.
Do Older and Young Adults Learn to Integrate Geometry While Navigating in an Environment of a Serious Game?
Authors: Kimura Kazushige , Moussavi Zahra
Published: January 2021

We evaluated the outcomes of an intervention using a serious game designed to be played on iPads for improving spatial reorientation by training users to integrate geometry of the environment, instead of relying solely on featural cues. Using data logged online through a clinical study of using this game, the effect of training among 16 older adults (69.3 ± 6.4 years, 4 males), who played the game repeatedly (self-administered) over a period of 8 weeks, was investigated. The game contains a hexagonal room with 3 objects, textured walls, and grids on the floor, which are removed one by one as the participant played the game. In each level, the room also rotates such that the viewpoint of the user is different from that of the previous level. Participants cannot play a higher level unless they make no mistake during the trials of the lower test level. In addition to data of older adults available from that clinical trial, we recruited 16 young adults (27.3 ± 5.6 years, 4 males) to play the game for 5 sessions and compared their results with those of the older adults. We evaluated the error type made in each test level and the scores for each session among older adults. Further, we compared the frequency of each error type between young and older adults during the test levels that a landmark adjacent to the target was removed over the first 5 sessions. The results of older adults’ performance suggest they learned to make fewer mistakes over the sessions. Also, both young and older adults learned to integrate the geometrical cues rather than relying on the landmark cue adjacent to the target to find the target. Overall, the results indicate the designed hexagonal room game can enhance spatial cognition among all age groups of adults.
Study on normalization of residual displacements for single-degree-of-freedom systems
Authors: Feng Zhibin , Gong Jinxin
Published: January 2021

Residual displacement spectrum is one of the most important means to predict the permanent deformation of structures after the earthquake, and various normalizations of residual displacements have generally been used for construction of the spectrum. However, the issue regarding the merits and drawbacks of each normalization has not yet been investigated thoroughly. A comparison between two normalizations that relate the residual displacements to the elastic and inelastic displacements is made in terms of the effect of ground motion and structural characteristics by means of the results of nonlinear time history analysis. The statistical results reveal that the residual-to-peak-inelastic displacement ratios have the advantages of small dispersion, samples without any outliers, and relatively symmetric distribution, which benefits from the strong correlation between residual and peak inelastic displacements. Moreover, the residual-to-peak-inelastic displacement ratios are almost independent of site conditions, significant duration, and natural periods. Consequently, the peak inelastic displacements are superior to the elastic ones as an intermediate step for residual displacements estimation, provided that the peak inelastic displacements are estimated with a low uncertainty. For providing alternatives to estimate residual displacement demands, the constant-strength residual displacement spectra are developed for both normalizations.
Quantifying nuisance ground motion thresholds for induced earthquakes
Authors: Schultz Ryan , Quitoriano Vince , Wald David J , Beroza Gregory C
Published: January 2021

Hazards from induced earthquakes are a growing concern with a need for effective management. One aspect of that concern is the “nuisance” from unexpected ground motions, which have the potential to cause public alarm and discontent. In this article, we borrow earthquake engineering concepts to quantify the chance of building damage states and adapt them to quantify felt thresholds for induced earthquakes in the Central and Eastern United States. We compare binary data of felt or not-felt reports from the “Did You Feel It” database with ShakeMap ground motion intensity measures (IM) for ∼360 earthquakes. We use a Monte Carlo logistic regression to discern the likelihood of perceiving various degrees of felt intensity, given a particular IM. These best-fit nuisance functions are reported in this article and are readily transferable. Of the shaking types considered, we find that peak ground velocity tends to be the best predictor of a felt earthquake. We also find that felt thresholds tended to decrease with increasing earthquake magnitude, after M ∼3.9. We interpret this effect as related to the duration of the event, where events smaller than M 3.9 are perceived as “impulsive” to the human senses. Improved quantification of the nuisance from induced earthquake ground motions could be utilized in management of the public perception of their causal operations. Although aimed at anthropogenic earthquakes, thresholds we derive could be useful in other realms, such as establishing best practices and protocols for earthquake early warning.
A Review of Actuation and Sensing Mechanisms in MEMS-Based Sensor Devices
Authors: Algamili Abdullah Saleh , Khir Mohd Haris Md. , Dennis John Ojur , Ahmed Abdelaziz Yousif , Alabsi Sami Sultan , Ba Hashwan Saeed Salem , Junaid Mohammed M.
Published: January 2021

Over the last couple of decades, the advancement in Microelectromechanical System (MEMS) devices is highly demanded for integrating the economically miniaturized sensors with fabricating technology. A sensor is a system that detects and responds to multiple physical inputs and converting them into analogue or digital forms. The sensor transforms these variations into a form which can be utilized as a marker to monitor the device variable. MEMS exhibits excellent feasibility in miniaturization sensors due to its small dimension, low power consumption, superior performance, and, batch-fabrication. This article presents the recent developments in standard actuation and sensing mechanisms that can serve MEMS-based devices, which is expected to revolutionize almost many product categories in the current era. The featured principles of actuating, sensing mechanisms and real-life applications have also been discussed. Proper understanding of the actuating and sensing mechanisms for the MEMS-based devices can play a vital role in effective selection for novel and complex application design.
Comprehensive analysis of ceRNA network related to lincRNA in glioblastoma and prediction of clinical prognosis
Authors: Liu Guangdong , Liu Danian , Huang Jingjing , Li Jianxin , Wang Chuang , Liu Guangyao , Ge Shiqiang , Gong Haidong
Published: January 2021

Background

Long intergenic non-coding RNAs (lincRNAs) are capable of regulating several tumours, while competitive endogenous RNA (ceRNA) networks are of great significance in revealing the biological mechanism of tumours. Here, we aimed to study the ceRNA network of lincRNA in glioblastoma (GBM).

Methods

We obtained GBM and normal brain tissue samples from TCGA, GTEx, and GEO databases, and performed weighted gene co-expression network analysis and differential expression analysis on all lincRNA and mRNA data. Subsequently, we predicted the interaction between lincRNAs, miRNAs, and target mRNAs. Univariate and multivariate Cox regression analyses were performed on the mRNAs using CGGA data, and a Cox proportional hazards regression model was constructed. The ceRNA network was further screened by the DEmiRNA and mRNA of Cox model.

Results

A prognostic prediction model was constructed for patients with GBM. We assembled a ceRNA network consisting of 18 lincRNAs, 6 miRNAs, and 8 mRNAs. Gene Set Enrichment Analysis was carried out on four lincRNAs with obvious differential expressions and relatively few studies in GBM.

Conclusion

We identified four lincRNAs that have research value for GBM and obtained the ceRNA network. Our research is expected to facilitate in-depth understanding and study of the molecular mechanism of GBM, and provide new insights into targeted therapy and prognosis of the tumour.
Association of PICK1 and BDNF variations with increased risk of methamphetamine dependence among Iranian population: a case–control study
Authors: Tajbakhsh Amir , Alimardani Maliheh , Asghari Mahla , Abedini Soheila , Saghafi Khadem Sohrab , Nesaei Bajestani Abolfazl , Alipoor Forough , Alidoust Maryam , Savardashtaki Amir , Hashemian Peyman , Pasdar Alireza
Published: January 2021

Background

Genetic factors play an important role in susceptibility to methamphetamine dependency. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a case–control study, we investigated the association between polymorphisms ofPICK1andBDNFgenes and methamphetamine dependence in an Iranian population.

Methods

Total of 235 cases and 204 controls were recruited in a period between 2015 to 2018. ThePICK1-rs713729,-rs2076369andBDNF-rs6265genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0.

Results

In the present study, two polymorphisms includingPICK1-rs713729(OR 1.38 (CI 1.08–1.52;P-value0.004) in multiplicative and dominant models, andPICK1-rs2076369(OR 1.31 (CI 1.10–1.56;P-value0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis showed a significant association of haplotype AG (OR 2.50 (CI 1.50–4.16;P-value0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR 0.67 (CI 0.50–0.91;P-value0.009) in dominant and co-dominant models with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium.

Conclusion

Our findings indicate that thePICK1gene polymorphism might affect the risk of methamphetamine dependency in our population.
A 10-year follow‐up on the practice of luteal phase support using worldwide web‐based surveys
Authors: Shoham Gon , Leong Milton , Weissman Ariel
Published: January 2021

Background

It has been demonstrated that luteal phase support (LPS) is crucial in filling the gap between the disappearance of exogenously administered hCG for ovulation triggering and the initiation of secretion of endogenous hCG from the implanting conceptus. LPS has a pivotal role of in establishing and maintaining in vitro fertilization (IVF) pregnancies. Over the last decade, a plethora of studies bringing new information on many aspects of LPS have been published. Due to lack of consent between researchers and a dearth of robust evidence-based guidelines, we wanted to make the leap from the bench to the bedside, what are the common LPS practices in fresh IVF cycles compared to current evidence and guidelines? How has expert opinion changed over 10 years in light of recent literature?

Methods

Over a decade (2009–2019), we conducted 4 web-based surveys on a large IVF-specialist website on common LPS practices and controversies. The self-report, multiple-choice surveys quantified results by annual IVF cycles.

Results

On average, 303 IVF units responded to each survey, representing, on average, 231,000 annual IVF cycles. Most respondents in 2019 initiated LPS on the day of, or the day after egg collection (48.7 % and 36.3 %, respectively). In 2018, 72 % of respondents administered LPS for 8–10 gestational weeks, while in 2019, 65 % continued LPS until 10–12 weeks. Vaginal progesterone is the predominant delivery route; its utilization rose from 64 % of cycles in 2009 to 74.1 % in 2019. Oral P use has remained negligible; a slight increase to 2.9 % in 2019 likely reflects dydrogesterone’s introduction into practice. E2 and GnRH agonists are rarely used for LPS, as is hCG alone, limited by its associated risk of ovarian hyperstimulation syndrome (OHSS).

Conclusions

Our Assisted reproductive technology (ART)-community survey series gave us insights into physician views on using progesterone for LPS. Despite extensive research and numerous publications, evidence quality and recommendation levels are surprisingly low for most topics. Clinical guidelines use mostly low-quality evidence. There is no single accepted LPS protocol. Our study highlights the gaps between science and practice and the need for further LPS research, with an emphasis on treatment individualization.
Wild food plants and fungi sold in the markets of Luang Prabang, Lao PDR
Authors: Łuczaj Łukasz , Lamxay Vichith , Tongchan Khamphart , Xayphakatsa Kosonh , Phimmakong Kongchay , Radavanh Somphavanh , Kanyasone Villapone , Pietras Marcin , Karbarz Małgorzata
Published: January 2021

Background

Open air markets hold an important position for ethnobiologists. In Southeast Asia, they are seriously understudied, in spite of their incredible biocultural diversity. In order to fill this gap we recorded plants and fungi sold in the open air markets of Luang Prabang, Lao PDR.

Methods

The markets were visited 38 times in four seasons: the dry season, early monsoon, mid-monsoon, and end-of-monsoon, at least 8 times per season. All items were photographed and voucher specimens were collected. Fungi were identified using DNA barcoding techniques.

Results

We recorded 110 species of wild edible plants and 54 species of fungi, including 49 wild-collected species. The sold plants included 86 species of green vegetables, 18 species of fruits and 3 species of flowers. Products from woody species constitute around half of all taxa sold. These include the young shoots of tree leaves, which are used for salads—an interesting feature of Lao cuisine. A large number of extremely rare Russula, with no reference sequences represented in databases or even species unknown to science is present on sale in the markets.

Conclusions

Luang Prabang markets are some of the richest in species of wild edible plants and fungi in Asia, and indeed in the whole world. It is worth pointing out the exceptionally long list of wild edible mushrooms which are sold in Luang Prabang (and probably elsewhere in Laos). We view the Morning Market of Luang Prabang as a cultural treasure that unites the traditions of eating a large number of living species with very diverse flora and fauna. Measures should be taken to strike a balance between local foraging traditions and nature conservation priorities.
A positive feedback loop between TAZ and miR-942-3p modulates proliferation, angiogenesis, epithelial-mesenchymal transition process, glycometabolism and ROS homeostasis in human bladder cancer
Authors: Wang Feifan , Fan Mengjing , Zhou Xuejian , Yu Yanlan , Cai Yueshu , Wu Hongshen , Zhang Yan , Liu Jiaxin , Huang Shihan , He Ning , Hu Zhenghui , Ding Guoqing , Jin Xiaodong
Published: January 2021

Background

Transcriptional coactivator with PDZ-binding motif (TAZ) has been reported to be involved in tumor progression, angiogenesis, epithelial-mesenchymal transition (EMT), glycometabolic modulation and reactive oxygen species (ROS) buildup. Herein, the underlying molecular mechanisms of the TAZ-induced biological effects in bladder cancer were discovered.

Methods

qRT-PCR, western blotting and immunohistochemistry were performed to determine the levels of TAZ in bladder cancer cells and tissues. CCK-8, colony formation, tube formation, wound healing and Transwell assays and flow cytometry were used to evaluate the biological functions of TAZ, miR-942-3p and growth arrest-specific 1 (GAS1). QRT-PCR and western blotting were used to determine the expression levels of related genes. Chromatin immunoprecipitation and a dual-luciferase reporter assay were performed to confirm the interaction between TAZ and miR-942. In vivo tumorigenesis and colorimetric glycolytic assays were also conducted.

Results

We confirmed the upregulation and vital roles of TAZ in bladder cancer. TAZ-induced upregulation of miR-942-3p expression amplified upstream signaling by inhibiting the expression of large tumor suppressor 2 (LATS2, a TAZ inhibitor). MiR-942-3p attenuated the impacts on cell proliferation, angiogenesis, EMT, glycolysis and ROS levels induced by TAZ knockdown. Furthermore, miR-942-3p restrained the expression of GAS1 to modulate biological behaviors.

Conclusion

Our study identified a novel positive feedback loop between TAZ and miR-942-3p that regulates biological functions in bladder cancer cells via GAS1 expression and illustrated that TAZ, miR-942-3p and GAS1 might be potential therapeutic targets for bladder cancer treatment.
Safety and efficacy of normobaric oxygenation on rescuing acute intracerebral hemorrhage-mediated brain damage—a protocol of randomized controlled trial
Authors: Chen Zhiying , Ding Jiayue , Wu Xiaoqin , Bao Bing , Cao Xianming , Wu Xiangbin , Yin Xiaoping , Meng Ran
Published: January 2021

Background

All of the existing medication and surgical therapies currently cannot completely inhibit intracerebral hemorrhage (ICH)-mediated brain damage, resulting in disability in different degrees in the involved patients. Normobaric oxygenation (NBO) was reported attenuating ischemic brain injury. Herein, we aimed to explore the safety and efficacy of NBO on rescuing the damaged brain tissues secondary to acute ICH, especially those in the perihematoma area being threatened by ischemia and hypoxia.

Methods

A total of 150 patients confirmed as acute spontaneous ICH by computed tomography (CT) within 6 h after symptoms onset, will enroll in this study after signing the informed consent, and enter into the NBO group or control group randomly according to a random number. In the NBO group, patients will inhale high-flow oxygen (8 L/min, 1 h each time for 6 cycles daily) and intake low-flow oxygen (2 L/min) in intermittent periods by mask for a total of 7 days. While in the control group, patients will breathe in only low-flow oxygen (2 L/min) by mask for 7 consecutive days. Computed tomography and perfusion (CT/CTP) will be used to evaluate cerebral perfusion status and brain edema. CT and CTP maps in the two groups at baseline and day 7 and 14 after NBO or low-flow oxygen control will be compared. The primary endpoint is mRS at both Day14 post-ICH and the end of the 3rd month follow-up. The secondary endpoints include NIHSS and plasma biomarkers at baseline and Day-1, 7, and 14 after treatment, as well as the NIHSS at the end of the 3rd month post-ICH and the incidence of bleeding recurrence and the mortalities within 3 months post-ICH.

Discussion

This study will provide preliminary clinical evidence about the safety and efficacy of NBO on correcting acute ICH and explore some mechanisms accordingly, to offer reference for larger clinical trials in the future.

Trial registration

ClinicalTrials.govNCT04144868. Retrospectively registered on October 29, 2019.
Visuo-postural dependency index (VPDI) in human postural control
Authors: Danna-dos-Santos Alessander , Ribeiro dos Santos Maria M. , Magalhães Alessandra T. , Cardoso Vinicius S. , Driusso Patricia , Mochizuki Luis , Degani Adriana M.
Published: January 2021

Background

Computerized stabilometry has been utilized to investigate the effect of vision on the neuromechanisms of human postural control. However, this approach lacks operational methods to quantify visual dependency during upright stance. This study had three goals: (1) To introduce the concept of visuo-postural dependency indices (VPDI) representing balance sway characteristics in multiple analytical domains (spatial, temporal, frequency, and structural), (2) To investigate the age and gender effects onVPDIs, and (3) To investigate the degree of relationships betweenVPDIand both subjective visual vertical and horizontal perception (SVVandSVH, respectively).

Methods

102 participants (16 to 80 years old) performed bipedal stances on a force platform with eyes open and closed. Response variables included theVPDIscomputed for each postural index. In addition, 29 participants also performedSVVandSVHassessments.

Results

FifteenVPDIs showed to be robust indicators of visual input modulation, and the variation across their magnitudes of modulation revealed a non-homogeneous response to changes in visual stimuli. Gender and age were not found to be a significant factor toVPDImodulation.

Conclusions

VPDIsrevealed to be potential measures capable to quantitatively assess visuo-postural dependency and aid the assessment of fall risks and balance impairments.
The clinical value of ficolin-3 gene polymorphism in rheumatic heart disease. An Egyptian adolescents study
Authors: Gomaa Maher H. , Khidr Emad Gamil , Elshafei Ahmed , Hamza Hala S. , Fattouh Aya M. , El-Husseiny Ahmed A. , Aglan Ahmed , Eldeib Mahmoud Gomaa
Published: January 2021

Objective

Ficolin-3 is one of the innate immunity molecules that was thought to play a pivotal role in Streptococcus pyogenes autoimmunity and its complications; rheumatic fever (RF) and rheumatic heart disease (RHD). We aimed to disclose if there is an association between ficolin-3 (FCN3) gene polymorphisms (rs4494157 and rs10794501) and RF with or without RHD for the first time in Egyptian adolescents.

Results

Serum ficolin-3 level was significantly elevated in patients suffering from RF with and without RHD in comparison with control. Regarding FCN3 gene (rs4494157) polymorphism, a significant correlation was found between the A allele and the susceptibility to RF with or without RHD (OR = 2.93, P = 0.0002 and OR = 2.23, P = 0.008 respectively). Besides, AA homozygous genotype showed a significant association with RHD risk (OR = 3.47, P = 0.026). Patients carrying the A allele (CA + AA) had significantly higher serum ficolin-3 than those carrying the CC genotype (P ˂ 0.0001). While the frequency of (rs10794501) polymorphism revealed no significant differences between the controls and RF patients with or without RHD (OR = 1.43, P = 0.261 and OR = 1.48, P = 0.208 respectively).
Cell membrane rupture: a novel test reveals significant variations among different brands of tissue culture flasks
Authors: Tchao Ruy
Published: January 2021

Objectives

Loss of cytoplasmic molecules including protein controls, due to cell membrane rupture can cause errors and irreproducibility in research data. Previous results have shown that during the washing of a monolayer of cells with a balanced salt solution, the fluid force causes cell membrane rupture on some areas of the flasks/dishes. This fact shows the non-uniformity of the polystyrene surface in terms of cell culture. There is at present no simple test to monitor that surface. This paper presents a novel biologically based assay to determine the degree of heterogeneity of flasks supplied by various manufacturers.

Results

This paper shows that significant variation exists in polystyrene surface heterogeneity among several brands of tissue culture flasks, varying from 4 to 20% of the flask surface. There is also large variability within the production lot of a manufacturer. The assay method involves loading the cells with a cytoplasmic fluorescent marker that is released upon cell membrane rupture. Cell membrane rupture also causes the loss of marker proteins such as GAPDH used in Westernblots. This novel assay method can be used to monitor the batch consistency and the manufacturing process of flasks/dishes. It may also be used to test new biomaterials.
De novo adult acute myeloid leukemia with two new mutations in juxtatransmembrane domain of the FLT3 gene: a case report
Authors: Alarbeed Ismael F. , Wafa Abdulsamad , Moassass Faten , Al-Halabi Bassel , Al-Achkar Walid , Liehr Thomas , Aboukhamis Imad
Published: January 2021

Background

Approximately 30% of adult acute myeloid leukemia (AML) acquire within fms-like tyrosine kinase 3 gene (FLT3) internal tandem duplications (FLT3/ITDs) in their juxtamembrane domain (JMD). FLT3/ITDs range in size from three to hundreds of nucleotides, and confer an adverse prognosis. Studies on a possible relationship between of FLT3/ITDs length and clinical outcomes in those AML patients were inconclusive, yet.

Case presentation

Here we report a 54-year-old Arab male diagnosed with AML who had two FLT3-ITD mutations in addition to NPM1 mutation. Cytogenetic approaches (banding cytogenetics) and fluorescence in situ hybridization (FISH) using specific probes to detect translocations t(8;21), t(15;17), t(16;16), t(12;21), and deletion del(13q)) were applied to exclude chromosomal abnormalities. Molecular genetic approaches (polymerase chain reaction (PCR) and the Sanger sequencing) identified a yet unreported combination of two new mutations in FLT3-ITDs. The first mutation induced a frameshift in JMD, and the second led to a homozygous substitution of c.1836T>A (p.F612L) also in JMD. Additionally a NPM1 type A mutation was detected. The first chemotherapeutic treatment was successful, but 1 month after the initial diagnosis, the patient experienced a relapse and unfortunately died.

Conclusions

To the best of our knowledge, a combination of two FLT3-ITD mutations in JMD together with an NPM1 type A mutation were not previously reported in adult AML. Further studies are necessary to prove or rule out whether the size of these FLT3-ITDs mutations and potential other double mutations in FLT3-ITD are correlated with the observed adverse outcome.
Retraction Note: Solutions of the equilibrium equations with finite mass subject
Authors: Shi Junguo , Liao Yadong
Published: January 2021

This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s13660-021-02561-7
Information technology and addiction science: promises and challenges
Authors: Bertholet Nicolas , Cunningham John A.
Published: January 2021

Background

Information technology can be used to advance addiction science and clinical practice.

Main body

This special issue, “Information technology (IT) interventions to advance treatment for opioid and other addictions” presents studies that expand our understanding of IT intervention efficacy, patients’ perspectives, and how IT can be used to improve substance use health care and research. This editorial introduces the topics addressed in the special issue and focuses on some of the challenges that the field is currently facing, such as attrition and treatment retention, transferability of intervention paradigms, and the challenge to keep pace with rapidly changing technologies.

Conclusions

Increasing treatment reach is particularly crucial in the addiction field. IT empowers researchers and clinicians to reach large portions of the population who might not otherwise access standard treatment modalities, because of geographical limitations, logistical constraints, stigma, or other reasons. The use of information technology may help reduce the substance use treatment gap and contribute to public health efforts to diminish the impact of substance use and other addictive behaviors on population health.
External validation of the Surgical Outcome Risk Tool (SORT) in 3305 abdominal surgery patients in the independent sector in the UK
Authors: Oakland K. , Cosentino D. , Cross T. , Bucknall C. , Dorudi S. , Walker D.
Published: January 2021

Background

Assessing the risk of post-surgical mortality is a key component of pre-surgical planning. The Surgical Outcome Risk Tool (SORT) uses pre-operative variables to predict 30-day mortality. The aim of this study was to externally validate SORT in patients undergoing major abdominal surgery.

Methods

Data were collected from patients treated in five independent hospitals in the UK. Individualised SORT scores were calculated, and area under the receiver operating characteristic (AUROC) and precision-recall curves (PRC) plus 95% confidence intervals (CI) were drawn to test the ability of SORT to identify in-hospital death. Outcomes of patients with a SORT predicted risk of mortality of ≥ 5% (high risk) were compared to those with a predicted risk of < 5% (standard risk).

Results

The study population comprised 3305 patients, mean age 51 years, 2783 (84.2%) underwent elective surgery most frequently involving the colon (24.6%), or liver, pancreas or gallbladder (18.2%). Overall, 1551 (46.9%) patients were admitted to ICU and 29 (0.88%) died. The AUROC of SORT for discriminating patients at risk of death in hospital was 0.899 (95% CI 0.849 to 0.949) and the PRC 0.247. In total, 72 (2.18%) patients were stratified as high risk. There were more unplanned ICU admissions and deaths in this group compared to the standard risk group (25.0% and 3.3%, versus 3.1% and 0.5%, respectively).

Conclusion

We externally validated SORT in a large population of abdominal surgery patients. SORT performed well in patients with lower risk profiles, but underpredicted adverse outcomes in the higher risk group.
Psychometric properties of the Arabic version of the Basic Psychological Needs Satisfaction-Frustration Scale (BPNSFS)
Authors: Zayed Kashef N. , Omara Ehab N. , al-Rawahi Nasser Y. , al-Shamli Ali K. , al-Atiyah Asma A. , al-Haramleh Ahmad A. , Azab Mahmoud S. , al-Khasawneh Ghada M. , Hassan Mohammed A.
Published: January 2021

Background

The aim of this study is to validate the Arabic version of the Basic Psychological Need Satisfaction and Frustration Scale (BPNSFS), and to investigate the extent of its invariance across five Arab countries and gender.

Methods

A back-translated version of the BPNSFS, the second version of the Beck Depression Inventory (BDI-II), and the Warwick-Edinburgh Mental Well-Being Scale (WEMWBS) were administered to a sample consisting of 1082 undergraduate students affiliated with universities in five Arab countries (487 males and 595 females: Mage = 20.04 ± 1.87 years). The data of the BPNSFS were examined for univariate and multivariate normality using Shapiro–Wilk tests and Mardia’s coefficient, respectively. To evaluate and compare the four models with confirmatory factor analysis (CFA), we used the following goodness-of-fit indices: the chi-square value (χ2), comparative fit index (CFI), Tucker-Lewis index (TLI), Root Mean-Square Error of Approximation (RMSEA), and Baysian Information Criterion (BIC). A multi-group CFA [Byrne in Structural equation modeling with EQS: basic concepts, applications, and programming, Routledge, Abingdon, 2013] on the BPNSFS structure to examine its invariance across the five Arab countries and across genders.

Results

The results of confirmatory factor analysis supported the generalizability of the BPNSFS’s six-factor model to the five Arab countries. The relationships between the six psychological needs satisfaction and frustrations and both mental health and symptoms of depression provide additional evidence on the construct validity of the BPNSFS through cross cultural data. The findings of BPNSFS’s measurement invariance across males and females and across the five Arab countries help ensure that the latent means are comparable across these different groups.

Conclusions

The study concluded that the Arabic version of the BPNSFS which measures satisfaction and frustration of the three basic needs (autonomy, competency, and relatedness) is proved to be invariant across the five Arab countries and gender and can be used to compare the basic psychological needs in the Arab context.
Tannin-based extracts of Mimosa tenuiflora bark: features and prospecting as wood adhesives
Authors: Lopes Pedro Jorge Goes , Calegari Leandro , de Medeiros Silva Wagner Alex , Gatto Darci Alberto , de Medeiros Neto Pedro Nicó , de Melo Rafael Rodolfo , Bakke Ivonete Alves , de Avila Delucis Rafael , Missio André Luiz
Published: January 2021

Mimosa tenuiflora (Willd.) Poir. (MT) is an underutilized plant specie since its wood is mostly used for energy production. Nonetheless, the bark from this forest plant has a high amount of tannins and other valuable compounds. Tannins have high worldwide importance and, because of that, there is an increasing number of researches on biorefinery systems aiming at maximizing their exploitation. The present study evaluated tannins extracted from the MT bark and their use for producing wood adhesives. Three types of powdered tannins were extracted using different solvents: a pure aqueous solution, a 5% sodium hydroxide (NaOH) aqueous solution, and a 5% sodium bisulfite (NaHSO3) aqueous solution. Distilled water, wheat flour, and formaldehyde were also used as a solvent, a glue extender, and a catalyst, respectively. These adhesives were applied for bonding pine wood joints and their shear strengths were determined. All the MT-based adhesives showed high viscosities and, yielded glue lines with similar shear strengths and similar shear deformations if compared to each other. That tannin-based glue incorporated with the tannin extracted using NaOH or NaHSO3 stood out and yielded similar bonding performances if compared to a commercial adhesive applied as a positive control.