bluuebunny/crossref_metadata_2025 huggingface dataset:
Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS): Pathophysiology and Clinical Implications
Authors: Cabal-Herrera Ana Maria , Tassanakijpanich Nattaporn , Salcedo-Arellano Maria Jimena , Hagerman Randi J.
Published: June 2020

The fragile X-associated tremor/ataxia syndrome (FXTAS) is a neurodegenerative disorder seen in older premutation (55–200 CGG repeats) carriers of FMR1. The premutation has excessive levels of FMR1 mRNA that lead to toxicity and mitochondrial dysfunction. The clinical features usually begin in the 60 s with an action or intention tremor followed by cerebellar ataxia, although 20% have only ataxia. MRI features include brain atrophy and white matter disease, especially in the middle cerebellar peduncles, periventricular areas, and splenium of the corpus callosum. Neurocognitive problems include memory and executive function deficits, although 50% of males can develop dementia. Females can be less affected by FXTAS because of a second X chromosome that does not carry the premutation. Approximately 40% of males and 16% of female carriers develop FXTAS. Since the premutation can occur in less than 1 in 200 women and 1 in 400 men, the FXTAS diagnosis should be considered in patients that present with tremor, ataxia, parkinsonian symptoms, neuropathy, and psychiatric problems. If a family history of a fragile X mutation is known, then FMR1 DNA testing is essential in patients with these symptoms.
Semisynthetic Modification of Tau Protein with Di-Ubiquitin Chains for Aggregation Studies
Authors: Munari Francesca , Barracchia Carlo Giorgio , Parolini Francesca , Tira Roberto , Bubacco Luigi , Assfalg Michael , D’Onofrio Mariapina
Published: June 2020

Ubiquitin, a protein modifier that regulates diverse essential cellular processes, is also a component of the protein inclusions characteristic of many neurodegenerative disorders. In Alzheimer’s disease, the microtubule associated tau protein accumulates within damaged neurons in the form of cross-beta structured filaments. Both mono- and polyubiquitin were found linked to several lysine residues belonging to the region of tau protein that forms the structured core of the filaments. Thus, besides priming the substrate protein for proteasomal degradation, ubiquitin could also contribute to the assembly and stabilization of tau protein filaments. To advance our understanding of the impact of ubiquitination on tau protein aggregation and function, we applied disulfide-coupling chemistry to modify tau protein at position 353 with Lys48- or Lys63-linked di-ubiquitin, two representative polyubiquitin chains that differ in topology and structure. Aggregation kinetics experiments performed on these conjugates reveal that di-ubiquitination retards filament formation and perturbs the fibril elongation rate more than mono-ubiquitination. We further show that di-ubiquitination modulates tau-mediated microtubule assembly. The effects on tau protein aggregation and microtubule polymerization are essentially independent from polyubiquitin chain topology. Altogether, our findings provide novel insight into the consequences of ubiquitination on the functional activity and disease-related behavior of tau protein.
Multifaceted Roles of Mitochondrial Components and Metabolites in Metabolic Diseases and Cancer
Authors: Nakhle Jean , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2020

Mitochondria are essential cellular components that ensure physiological metabolic functions. They provide energy in the form of adenosine triphosphate (ATP) through the electron transport chain (ETC). They also constitute a metabolic hub in which metabolites are used and processed, notably through the tricarboxylic acid (TCA) cycle. These newly generated metabolites have the capacity to feed other cellular metabolic pathways; modify cellular functions; and, ultimately, generate specific phenotypes. Mitochondria also provide intracellular signaling cues through reactive oxygen species (ROS) production. As expected with such a central cellular role, mitochondrial dysfunctions have been linked to many different diseases. The origins of some of these diseases could be pinpointed to specific mutations in both mitochondrial- and nuclear-encoded genes. In addition to their impressive intracellular tasks, mitochondria also provide intercellular signaling as they can be exchanged between cells, with resulting effects ranging from repair of damaged cells to strengthened progression and chemo-resistance of cancer cells. Several therapeutic options can now be envisioned to rescue mitochondria-defective cells. They include gene therapy for both mitochondrial and nuclear defective genes. Transferring exogenous mitochondria to target cells is also a whole new area of investigation. Finally, supplementing targeted metabolites, possibly through microbiota transplantation, appears as another therapeutic approach full of promises.
Luminal STIM1 Mutants that Cause Tubular Aggregate Myopathy Promote Autophagic Processes
Authors: Sallinger Matthias , Tiffner Adéla , Schmidt Tony , Bonhenry Daniel , Waldherr Linda , Frischauf Irene , Lunz Victoria , Derler Isabella , Schober Romana , Schindl Rainer
Published: June 2020

Stromal interaction molecule 1 (STIM1) is a ubiquitously expressed Ca2+ sensor protein that induces permeation of Orai Ca2+ channels upon endoplasmic reticulum Ca2+-store depletion. A drop in luminal Ca2+ causes partial unfolding of the N-terminal STIM1 domains and thus initial STIM1 activation. We compared the STIM1 structure upon Ca2+ depletion from our molecular dynamics (MD) simulations with a recent 2D NMR structure. Simulation- and structure-based results showed unfolding of two α-helices in the canonical and in the non-canonical EF-hand. Further, we structurally and functionally evaluated mutations in the non-canonical EF-hand that have been shown to cause tubular aggregate myopathy. We found these mutations to cause full constitutive activation of Ca2+-release-activated Ca2+ currents (ICRAC) and to promote autophagic processes. Specifically, heterologously expressed STIM1 mutations in the non-canonical EF-hand promoted translocation of the autophagy transcription factors microphthalmia-associated transcription factor (MITF) and transcription factor EB (TFEB) into the nucleus. These STIM1 mutations additionally stimulated an enhanced production of autophagosomes. In summary, mutations in STIM1 that cause structural unfolding promoted Ca2+ down-stream activation of autophagic processes.
OMV Vaccines and the Role of TLR Agonists in Immune Response
Authors: Mancini Francesca , Rossi Omar , Necchi Francesca , Micoli Francesca
Published: June 2020

Outer Membrane Vesicles (OMVs) are bacterial nanoparticles that are spontaneously released during growth both in vitro and in vivo by Gram-negative bacteria. They are spherical, bilayered membrane nanostructures that contain many components found within the external surface of the parent bacterium. Naturally, OMVs serve the bacteria as a mechanism to deliver DNA, RNA, proteins, and toxins, as well as to promote biofilm formation and remodel the outer membrane during growth. On the other hand, as OMVs possess the optimal size to be uptaken by immune cells, and present a range of surface-exposed antigens in native conformation and Toll-like receptor (TLR) activating components, they represent an attractive and powerful vaccine platform able to induce both humoral and cell-mediated immune responses. This work reviews the TLR-agonists expressed on OMVs and their capability to trigger individual TLRs expressed on different cell types of the immune system, and then focuses on their impact on the immune responses elicited by OMVs compared to traditional vaccines.
EP3 Is an Independent Prognostic Marker Only for Unifocal Breast Cancer Cases
Authors: Zati Zehni Alaleh , Jeschke Udo , Hester Anna , Kolben Thomas , Ditsch Nina , Jacob Sven-Niclas , Mumm Jan-Niclas , Heidegger Helene Hildegard , Mahner Sven , Vilsmaier Theresa
Published: June 2020

The aim of this study was to evaluate the prognostic impact of prostaglandin E2 receptor 3 (EP3) receptor expression might have on the two different breast cancer entities: multifocal/multicentric versus unifocal. As the prognosis determining aspects, we investigated the overall- and disease-free survival by uni-and multivariate analysis. To underline the study’s conclusion, we additionally considered the histopathological grading and the tumor node metastasis (TNM) staging system. A retrospective statistical analysis was performed on survival related events in a series of 289 sporadic breast cancer (BC) patients treated at the Department of Obstetrics and Gynecology at the Ludwig–Maximillian’s University in Munich between 2000 and 2002. The EP3 receptor expression was analyzed by immunohistochemistry and showed to have a significantly positive association with breast cancer prognosis for both entities, although with major differences. Patients with unifocal BC with EP3 receptor expression showed a significant improved overall survival, in contrast to the patient cohort with multifocal/multicentric BC. In this group, EP3 expression revealed its positive impact merely five years after initial diagnosis. Underlining the positive influence of EP3 as a positive prognosticator notably for unifocal breast cancer, only this patient cohort showed favorable outcomes in staging and grading. Especially EP3 expression in unifocal breast cancer was identified as an independent prognostic marker for the overall survival, when adjusted for age, grading, and staging. Altogether, our results strengthen the need to further investigate the behavior of EP3 in breast cancer and understand why markers linked to inflammation show different effects on prognosis and clinicopathological parameters on each focality type.
Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes
Authors: Conese Massimo , Pace Lorenzo , Pignataro Nicoletta , Catucci Lucia , Ambrosi Antonio , Di Gioia Sante , Tartaglia Nicola , Liso Arcangelo
Published: June 2020

Recently, insulin-like growth factor binding protein 6 (IGFBP-6) has been shown to play a putative role in the immune system, as monocyte-derived dendritic cells (Mo-DCs) are stimulated by hyperthermia to express IGFBP-6 at both the mRNA and protein levels. However, the presence of IGFBP-6 in extracellular vesicles (EVs) and whether other pro-inflammatory stimuli can induce IGFBP-6 expression in Mo-DCs are not known yet. In this brief report, we show that hyperthermia (39 °C) induces IGFBP-6 secretion associated with microvesicles and exosomes as early as 3 h. Moreover, free IGFBP-6 is found in conditioned media (CM) of hyperthermia- and H2O2-treated Mo-DCs, but not in CM obtained from monocytes similarly treated. These results show that diverse inflammatory stimuli can induce IGFBP-6 association with EVs and secretion in conditioned medium, indicating a role for IGFBP-6 in communication between immune cells.
Cannabis, the Endocannabinoid System and Immunity—the Journey from the Bedside to the Bench and Back
Authors: Almogi-Hazan Osnat , Or Reuven
Published: June 2020

The Cannabis plant contains numerous components, including cannabinoids and other active molecules. The phyto-cannabinoid activity is mediated by the endocannabinoid system. Cannabinoids affect the nervous system and play significant roles in the regulation of the immune system. While Cannabis is not yet registered as a drug, the potential of cannabinoid-based medicines for the treatment of various conditions has led many countries to authorize their clinical use. However, the data from basic and medical research dedicated to medical Cannabis is currently limited. A variety of pathological conditions involve dysregulation of the immune system. For example, in cancer, immune surveillance and cancer immuno-editing result in immune tolerance. On the other hand, in autoimmune diseases increased immune activity causes tissue damage. Immuno-modulating therapies can regulate the immune system and therefore the immune-regulatory properties of cannabinoids, suggest their use in the therapy of immune related disorders. In this contemporary review, we discuss the roles of the endocannabinoid system in immunity and explore the emerging data about the effects of cannabinoids on the immune response in different pathologies. In addition, we discuss the complexities of using cannabinoid-based treatments in each of these conditions.
Gamification and Advanced Technology to Enhance Motivation in Education
Authors: Molina-Carmona Rafael , Llorens-Largo Faraón
Published: June 2020

The aim of this Special Issue is to compile a set of research works that highlight the use of gamification and other advanced technologies as powerful tools for motivation during learning. We have been fortunate to obtain a representative sample of the current research activity in this field.
Unique Fine Morphology of Mouthparts in Haematoloecha nigrorufa (Stål) (Hemiptera: Reduviidae) Adapted to Millipede Feeding
Authors: Wang Yan , Zhang Junru , Wang Wanshan , Brożek Jolanta , Dai Wu
Published: June 2020

Millipede assassin bugs are a diverse group of specialized millipede predators. However, the feeding behavior of Ectrichodiinae remains poorly known, especially how the mouthpart structures relate to various functions in feeding. In this study, fine morphology of the mouthparts and feeding performance of Haematoloecha nigrorufa (Stål, 1867) was observed and described in detail for the first time. The triangular labrum is divided by a conspicuous transverse membrane into a strongly sclerotized basilabrum and a less sclerotized distilabrum. Fifteen types of sensilla are distributed on the mouthparts. Each mandibular stylet has an expanded spatulate apex and about 150 approximately transverse ridges on the external middle side; these help in penetrating the ventral trunk area and the intersegmental membranes of millipede prey. The right maxilla is tapered. On the internal surface are a row dorsal short bristles near the apex and a row of ventral bristles preapically. A longitudinal row of long lamellate structures extend proximate for a considerable distance, lie entirely within the food canal, and bear several short spines and short bristles. There is no obvious difference between males and females in the distribution, number, and types of sensilla on mouthparts. The adult feeding process involves several steps, including searching and capturing prey, paralyzing prey, a resting phase, and a feeding phase. The evolution of the mouthpart morphology and the putative functional significance of their sensilla are discussed, providing insight into the structure and function of the mouthparts adapted for millipede feeding.
What Is Required for Edible Insects to Become Medical Food? From a Health Professionals and Caregivers’ Perspective
Authors: Jeong Harry , Shin Kwangsoo
Published: June 2020

The challenge in the edible insect industry is to reverse consumers’ aversion to insects, which is a barrier to their consumption. This requires innovation by users rather than producers. This study aimed to present how edible insects could be promoted as medical foods from the health professionals and caregivers’ perspective. By analyzing the characteristics of the medical foods market, this study found a niche market and plan to develop medical foods using edible insects as an alternative to meet the needs of consumers. The survey participants were caregivers, nurses, and doctors as providers of medical foods. Based on the survey results, this study proposed strategies to reduce consumers’ aversion to edible insects and increase their consumption. To promote insect medical foods, it is required to hold frequent insect-related events and use clean raw materials.
Preparation and Characterization of an Electrospun PLA-Cyclodextrins Composite for Simultaneous High-Efficiency PM and VOC Removal
Authors: Palmieri Silvia , Pierpaoli Mattia , Riderelli Luca , Qi Sheng , Ruello Maria Letizia
Published: June 2020

Electrospinning is known to be a facile and effective technique to fabricate fibers of a controlled diameter-distribution. Among a multitude of polymers available for the purpose, the attention should be addressed to the environmentally compatible ones, with a special focus on sustainability. Polylactic acid (PLA) is a widespread, non-toxic polymer, originating from renewable sources and it can degrade into innocuous products. While the production of fibrous membranes is attractive for airborne particles filtration applications, their impact on the removal of gaseous compounds is generally neglected. In this study, electrospun PLA-based nanofibers were functionalized with cyclodextrins, because of their characteristic hydrophobic central cavity and a hydrophilic outer surface, in order to provide adsorptive properties to the composite. The aim of this work is to investigate a hybrid composite, from renewable sources, for the combined filtration of particulate matter (PM) and adsorption of volatile organic compounds (VOCs). Results show how their inclusion into the polymer strongly affects the fiber morphology, while their attachment onto the fiber surface only positively affects the filtration efficiency.
Effects of Turbulence on the Vortex Modes Carried by Quasi-Diffracting Free Finite Energy Beam in Ocean
Authors: Liang Qiyong , Zhang Yixin , Yang Dongyu
Published: June 2020

By developing new wave structure function of a beam waves, we derive the transmitting probability of signal vortex modes in oceanic turbulence based on Rytov approximation theory. Applying this transmitting probability of signal vortex modes, we study the influence of oceanic turbulence on the transmittance of the vortex modes carried by Mathieu-Gaussian beam. This model shows the transmitting probability of Mathieu-Gaussian beam with narrow initial beam width, long wavelength, and small ellipticity parameter is higher than the transmitting probability of the signal vortex modes in case of the beam with wide initial beam width, short wavelength, and great ellipticity parameter. Furthermore, when Mathieu-Gaussian beam has a suitable semi-cone angle, the effect of weak-turbulence channel on the transmitting probability of signal vortex modes with different topological charge can be ignored. Mathieu-Gaussian beam is a more suitable carrier for high information channel of underwater wireless optical communication than Laguerre-Gaussian beam.
The Impact of Brand Relationships on Corporate Brand Identity and Reputation—An Integrative Model
Authors: Barros Teresa , Rodrigues Paula , Duarte Nelson , Shao Xue-Feng , Martins F. V. , Barandas-Karl H. , Yue Xiao-Guang
Published: June 2020

The current literature focuses on the cocreation of brands in dynamic contexts, but the impact of the relationships among brands on branding is poorly documented. To address this gap a concept is proposed concerning the relationships between brands and a model is developed, showing the influence of the latter on the identity and reputation of brands. Therefore, the goal of this study is to develop a brand relationships concept and to build a framework relating it with corporate brand identity and reputation, in a higher consumer involvement context like higher education. Structural equation modelling (SEM) was used for this purpose. In line with this, interviews, cooperatively developed by higher education lecturers and brand managers, were carried out with focus groups of higher education students, and questionnaires conducted, with 216 complete surveys obtained. Data are analyzed using confirmatory factor analysis and structural equation modelling. Results demonstrate that the concept of brand relationships comprises three dimensions: trust, commitment, and motivation. The structural model reveals robustness regarding the selected fit indicators, demonstrating that the relationships between brands influence brand identity and reputation. This suggests that managers must choose and promote brand relationships that gel with the identity and reputation of the primary brand they manage, to develop an integrated balanced product range.
Expectations and Experiences of Women Imprisoned for Drug Offending and Returning to Communities in Thailand: Understanding Women’s Pathways Into, Through, and Post-Imprisonment
Authors: Jeffries Samantha , Chuenurah Chontit , Russell Tristan
Published: June 2020

Thailand places a high priority on the gender-specific contexts out of which offending arises and the differential needs of women in the criminal justice system. Despite this, Thailand has the highest female incarceration rate in South East Asia and there has been substantial growth since the 1990s. This increase has been driven by punitive changes in drug law, criminal justice policy/practice which have disproportionately impacted women. As female representation in Thailand’s prisons grows, so does the number of women who return to communities. Thus, one of the challenges facing Thai society is the efficacious re-integration of growing numbers of formally incarcerated women. However, what is known about re-entry comes almost exclusively from studies of prisoners (usually men) returning home in western societies. Re-integration does not occur in a vacuum. Supporting women post-release necessitates knowledge of their pathways to, experiences of, and journeys out of prison. Utilising in-depth interviews with (n = 80) imprisoned/formally incarcerated women and focus groups with (n = 16) correctional staff, this paper reports findings from the first comprehensive study of women’s re-integration expectations and experiences in Thailand. Findings showed that women had multifaceted and intersectional needs which directed their pathways into, during, and out of prison.
Adsorption and Corrosion Performance of New Cationic Gemini Surfactants Derivatives of Fatty Amido Ethyl Aminium Chloride with Ester Spacer for Mild Steel in Acidic Solutions
Authors: Bin-Hudayb Nashwa S. , Badr Entsar E. , Hegazy M.A.
Published: June 2020

Three new cationic gemini surfactants with ester spacer type 2-2′-(ethane-1,2-diyl bis(oxy)) bis(N-(2-alkanamidoethyl)-N,N-dimethyl-2-oxoethan-1-aminium)) dichloride) (CGSES12, CGSES14 and CGSES16), based on N,N-dimethyl fatty amido ethylamine, were produced. These gemini quaternary ammonium salts were synthesized using a three-step reaction method, starting from th/e condensation of the fatty acid chloride (RCOCl) of various hydrophobic chain lengths (R, C11H23, C13H27, C15H31) with N,N-dimethyl ethylene diamine, followed by the quaternization of the tertiary amino group formed with the spacer of the ester group formed in the second step. The chemical configuration of the surfactants was established by FT-IR, 1HNMR, 13CNMR and Mass spectroscopies. The inhibition performance of three surfactants was studied by weight loss and electrochemical measurements. The results show that CGSES12, CGSES14 and CGSES16 behave as effective inhibitors and surface agents. The maximum efficiency was higher than 94% at 2.5 mM, and the inhibition order was CGSES16 > CGSES14 > CGSES12. This was due to the increment in hydrophobicity of the gemini surfactants. Their adsorption on a mild steel surface followed the Langmuir isotherm. CGSES12, CGSES14 and CGSES16 can be considered mixed-type inhibitors. The presence of CGSES12, CGSES14 and CGSES16 increased charge transfer resistance and decreased the corrosion rate. The adsorption focused on heteroatoms and the surface properties of cationic gemini surfactants.
Multiple Sensor Monitoring of CFRP Drilling to Define Cutting Parameters Sensitivity on Surface Roughness, Cylindricity and Diameter
Authors: Álvarez-Alcón Miguel , López de Lacalle Luis Norberto , Fernández-Zacarías Francisco
Published: June 2020

Machining parameters affects the final quality of components made in carbon fiber reinforced plastic (CFRP) composite materials. In this framework, the work here presented aims at studying the right combination of cutting speed (vc) and feed rate (vf), for dry drilling of carbon fiber reinforced plastic composite materials, which obtained better results regarding roughness, hole cylindricity, and diameter. A series of experimental tests were carried out under different drilling conditions (vc/vf), monitoring the thrust force (Fz), torque (T), and electric power (EP), to define which one can help more for industrial daily life production. Results validation was carried out using the analysis of variance, in order to relate main machining parameters cutting speed and linear feed, with thrust force, drilling torque, main spindle electric power and hole quality parameters (average roughness, cylindricity and diameter). The conclusions show that thrust force is not proportional to the cutting speed and the best combinations of cutting speed and feed were found out around the average values of tested parameters. Spindle electric power is an interesting element to take into account because it is easy to consider in real production.
Strengthening the Structural Behavior of Web Openings in RC Deep Beam Using CFRP
Authors: Rahim Nurul Izzati , Mohammed Bashar S. , Al-Fakih Amin , Wahab M. M. A. , Liew M. S. , Anwar Abdullah , Amran Y. H. Mugahed
Published: June 2020

Deep beams are more susceptible to shear failure, and therefore reparation is a crucial for structural reinforcements. Shear failure is structural concrete failure in nature. It generally occurs without warning; however, it is acceptable for the beam to fail in bending but not in shear. The experimental study presented the structural behavior of the deep beams of reinforced concrete (RC) that reinforces the web openings with externally connected carbon fiber reinforced polymer (CFRP) composite in the shear zone. The structural behavior includes a failure mode, and cracking pattern, load deflection responses, stress concentration and the reinforcement factor were investigated. A total of nine reinforced concrete deep beams with openings strengthened with CFRP and one control beam without an opening have been cast and tested under static four-point bending load till failure. The experimental results showed that the increase the size of the opening causes an increase in the shear strength reduction by up to 30%. Therefore, the larger the openings, the lower the capability of load carriage, in addition to an increase in the number of CFRP layers that could enhance the load carrying capacity. Consequently, utilization of the CFRP layer wrapping technique strengthened the shear behavior of the reinforced concrete deep beams from about 10% to 40%. It was concluded that the most effective number of CFRP layers for the deep beam with opening sizes of 150 mm and 200 mm were two layers and three layers, respectively.
Accuracy Evaluation of 14 Maxillary Full Arch Implant Treatments Performed with Da Vinci Bridge: A Case Series
Authors: Stefanelli Luigi V. , Mandelaris George A. , Franchina Alessio , Di Nardo Dario , Galli Massimo , Pagliarulo Michele , Testarelli Luca , Di Carlo Stefano , Gambarini Gianluca
Published: June 2020

The use of pterygoid implants can be an attractive alternative to sinus bone grafting in the treatment of posterior atrophic maxilla. This technique has not been widely used because of the difficulty of the surgical access, the presence of vital structures, and the prosthetic challenges. The use of dynamic computer aided implantology (DCAI) allows the clinician to utilize navigation dental implant surgery, which allows the surgeon to follow the osteotomy site and implant positioning in real time. A total of 14 patients (28 pterygoid implants and 56 intersinusal implants) were enrolled in the study for a full arch implant prosthetic rehabilitation (4 frontal implants and 2 pterygoids implants), using a dynamic navigation system. The reported accuracy of pterygoid implants inserted using DCAI was 0.72 mm at coronal point, 1.25 mm at apical 3D, 0.66 mm at apical depth, and 2.86° as angular deviation. The use of pterygoid implants in lieu of bone grafting represents a valid treatment opportunity to carry out a safe, accurate, and minimally invasive surgery, while reducing treatment time and avoiding cantilevers for a full implant prosthetic rehabilitation of the upper arch.
Nanophotosensitizers for Folate Receptor-Targeted and Redox-Sensitive Delivery of Chlorin E6 against Cancer Cells
Authors: Kook Min-Suk , Lee Chang-Min , Jeong Young-Il , Kim Byung-Hoon
Published: June 2020

In this study, FA–PEG3500-ss-Ce6tri copolymer was synthesized to deliver photosensitizers via redox-sensitive and folate receptor-specific manner. Folic acid (FA) was attached to amine end of poly (ethylene glycol) (PEG3500) (FA–PEG3500 conjugates) and cystamine-conjugated chlorin e6 (Ce6) (Ce6-cystamine conjugates). FA–PEG3500 was further conjugated with Ce6-cystamine to produce FA–PEG3500-ss-Ce6 conjugates. To the remaining amine end group of Ce6-cystamine conjugates, Ce6 was attached to produce FA–PEG3500-ss-Ce6tri. Nanophotosensitizers of FA–PEG3500-ss-Ce6tri copolymer were smaller than 200 nm. Their shapes were disintegrated by treatment with GSH and then Ce6 released by GSH-dependent manner. Compared to Ce6 alone, FA–PEG3500-ss-Ce6tri copolymer nanophotosensitizers recorded higher Ce6 uptake ratio, reactive oxygen species (ROS) production and cellular cytotoxicity against KB and YD-38 cells. The in vitro and in vivo study approved that delivery of nanophotosensitizers is achieved by folate receptor-sensitive manner. These results indicated that FA–PEG3500-ss-Ce6tri copolymer nanophotosensitizers are superior candidate for treatment of oral cancer.
Synthesis and Effects of Two Novel Rare-Earth Energetic Complexes on Thermal Decomposition of Cyclotetramethylene Tetranitramine (HMX)
Authors: Cao Xuefang , Wei Zhixian , Song Jiangfeng , Zhang Hedan , Qu Yuanyuan , Xie Fei
Published: June 2020

In order to explore the effect of the energetic complex on the thermal decomposition HMX, two new rare-earth energetic complexes [La(tza)(NO3)2(H2O)4]n (1) and [Ce(tza)(NO3)2(H2O)4]n (2) (Htza = tetrazole-1-acetic acid) were prepared by a solvent evaporation method. The obtained products were structurally characterized by Fourier-transform infrared spectroscopy (FTIR), elemental analysis, powder X-ray diffraction (PXRD), single crystal X-ray diffraction (XRD), and thermogravimetric analysis coupled with differential scanning calorimetry (TG-DSC). In addition, the compatibility of complex 1 with cyclotetramethylene tetranitramine (HMX) was studied by DSC and FTIR, respectively. Structural analysis suggested that complex 1 exhibits an orthorhombic, P 21 21 21 space group, and the La (III) ion was 10-fold coordinated in a distorted double-capped antiprism configuration. Complex 2 featured a one-dimensional, right-handed helical infinite chain. The effect of complexes 1 and 2 on the thermal decomposition of HMX was investigated by DSC, which revealed that complex 1 showed a slightly better effect than 2 on the thermal decomposition of HMX and released more heat. Furthermore, complex 1 had good compatibility with HMX, indicating that it may act as a combustion promoter for nitrate ester plasticized polyether (NEPE) solid propellant.
Tuning the Photo-Luminescence Properties of WO3 Layers by the Adjustment of Layer Formation Conditions
Authors: Petruleviciene Milda , Juodkazyte Jurga , Parvin Maliha , Tereshchenko Alla , Ramanavicius Simonas , Karpicz Renata , Samukaite-Bubniene Urte , Ramanavicius Arunas
Published: June 2020

In this research we have applied sol-gel synthesis for the deposition of tungsten (VI) oxide (WO3) layers using two different reductants (ethanol and propanol) and applying different dipping times. WO3 samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR), photoluminescence (PL) and time-resolved photoluminescence decay methods. Photoelectrochemical (PEC) behaviour of synthesized coatings was investigated using cyclic voltammetry in the dark and under illumination. Formation of different structures in differently prepared samples was revealed and significant differences in the PL spectra and PEC performance of the samples were observed. The results showed that reductant used in the synthesis and dipping time strongly influenced photo-electrochemical properties of the coatings. Correlation between the morphology, PL and PEC behaviour has been explained.
Luminescent Studies on Germanate Glasses Doped with Europium Ions for Photonic Applications
Authors: Żmojda Jacek , Kochanowicz Marcin , Miluski Piotr , Golonko Piotr , Baranowska Agata , Ragiń Tomasz , Dorosz Jan , Kuwik Marta , Pisarski Wojciech , Pisarska Joanna , Szal Renata , Mach Gabriela , Starzyk Bartosz , Leśniak Magdalena , Sitarz Maciej , Dorosz Dominik
Published: June 2020

Glass and ceramic materials doped with rare earth (RE) ions have gained wide interest in photonics as active materials for lasers, optical amplifiers, and luminescent sensors. The emission properties of RE-doped glasses depend on their chemical composition, but they can also be tailored by modifying the surrounding active ions. Typically, this is achieved through heat treatment (including continuous-wave and pulsed lasers) after establishing the ordering mechanisms in the particular glass–RE system. Within the known systems, silicate glasses predominate, while much less work relates to materials with lower energy phonons, which allow more efficient radiation sources to be constructed for photonic applications. In the present work, the luminescent and structural properties of germanate glasses modified with phosphate oxide doped with Eu3+ ions were investigated. Europium dopant was used as a “spectroscopic probe” in order to analyze the luminescence spectra, which characterizes the changes in the local site symmetries of Eu3+ ions. Based on the spectroscopic results, a strong influence of P2O5 content was observed on the excitation and luminescence spectra. The luminescence study of the most intense 5D0→7F2 (electric dipole) transition revealed that the increase in the P2O5 content leads to the linewidth reduction (from 15 nm to 10 nm) and the blue shift (~2 nm) of the emission peak. According to the crystal field theory, the introduction of P2O5 into the glass structure changes the splitting number of sublevels of the 5D0→7F1 (magnetic dipole) transition, confirming the higher polymerization of fabricated glass. The slightly different local environment of Eu3+ centers the results in a number of sites and causes inhomogeneous broadening of spectral lines. It was found that the local asymmetry ratio estimated by the relation of (5D0→7F2)/(5D0→7F1) transitions also confirms greater changes in local symmetry around Eu3+ ions. Our results indicate that modification of germanate glass by P2O5 allows control of their structural properties in order to functionalize the emissions for application as luminescent light sources and sensors.
Chitosan-Based Bioactive Glass Gauze: Microstructural Properties, In Vitro Bioactivity, and Biological Tests
Authors: Sergi Rachele , Bellucci Devis , Salvatori Roberta , Cannillo Valeria
Published: June 2020

Passive commercial gauzes were turned into interactive wound dressings by impregnating them with a chitosan suspension. To further improve healing, and cell adhesion and proliferation, chitosan/bioactive glass wound dressings were produced with the addition of (i) 45S5, (ii) a Sr- and Mg-containing bioactive glass, and (iii) a Zn-containing bioactive glass to the chitosan suspension. SEM and FTIR analyses evidenced positive results in terms of incorporation of bioactive glass particles. Bioactivity was investigated by soaking chitosan-based bioactive glass wound dressings in simulated body fluid (SBF). Cell viability, proliferation, and morphology were investigated using NIH 3T3 (mouse embryonic fibroblast) cells by neutral red (NR) uptake and MTT assays. Furthermore, the wound-healing rate was evaluated by means of the scratch test, using NIH 3T3. The results showed that bioactive glass particles enhance cell adhesion and proliferation, and wound healing compared to pure chitosan. Therefore, chitosan-based bioactive glass wound dressings combine the properties of the organic matrix with the specific biological characteristics of bioactive glasses to achieve chitosan composites suitable for healing devices.
Parameter Estimation of Induction Machine Single-Cage and Double-Cage Models Using a Hybrid Simulated Annealing–Evaporation Rate Water Cycle Algorithm
Authors: Ćalasan Martin , Micev Mihailo , Ali Ziad M. , Zobaa Ahmed F. , Abdel Aleem Shady H. E.
Published: June 2020

This paper presents the usage of the hybrid simulated annealing—evaporation rate water cycle algorithm (SA-ERWCA) for induction machine equivalent circuit parameter estimation. The proposed algorithm is applied to nameplate data, measured data found in the literature, and data measured experimentally on a laboratory three-phase induction machine operating as an induction motor and as an induction generator. Furthermore, the proposed method is applied to both single-cage and double-cage equivalent circuit models. The accuracy and applicability of the proposed SA-ERWCA are intensively investigated, comparing the machine output characteristics determined by using SA-ERWCA parameters with corresponding characteristics obtained by using parameters determined using known methods from the literature. Also, the comparison of the SA-ERWCA with classic ERWCA and other algorithms used in the literature for induction machine parameter estimation is presented. The obtained results show that the proposed algorithm is a very effective and accurate method for induction machine parameter estimation. Furthermore, it is shown that the SA-ERWCA has the best convergence characteristics compared to other algorithms for induction machine parameter estimation in the literature.
Global Longitudinal Strain of the Systemic Ventricle Is Correlated with Plasma Galectin-3 and Predicts Major Cardiovascular Events in Adult Patients with Congenital Heart Disease
Authors: Frogoudaki Alexandra A. , Pantelakis Ioannis , Bistola Vasiliki , Kroupis Christos , Birba Dionysia , Ikonomidis Ignatios , Alexopoulos Dimitrios , Filippatos Gerasimos , Parissis John
Published: June 2020

Backround and Objective: We sought to assess in adult congenital heart disease (ACHD) patients the prognostic value of plasma galectin-3 (Gal-3) levels and systemic ventricular global longitudinal strain (SV GLS) as well as their association with NTproBNP and arrhythmogenesis. Materials and Methods: We studied 58 patients (26 men, mean age 37 ± 16.8 years) with various congenital heart diseases. Patients underwent echocardiogram, 24 h ambulatory ECG monitoring, while NTproBNP and Gal-3 were measured. They were followed up (median of 790.5 days -IQR 350.3 days) and major cardiovascular events (MACE) were recorded. Results. Mean Gal-3 levels were 17.07 ± 6.38 ng/m. Plasma Gal-3 was correlated with LogNTproBNP (r = 0.456, p = 0.001).Gal-3 levels associated with supraventricular tachycardia (SVT) (p < 0.001) and ventricular tachycardia (VT) (p < 0.001), but was not associated with MACE (HR 1.018, 95% CI 0.944–1.098, p = 0.641).Mean SVGLS in patients with systemic left ventricle was −15.91% ± 4.09%, which was significantly lower compared to patients with systemic right ventricle and patients with single ventricle (−11.42% ± 3.37% and −11.9% ± 5.06%, respectively, p = 0.021).SV GLS correlated with plasma Gal-3 (r = 0.313, p = 0.027) and logNTproBNP (r = 0.479, p < 0.001). SVGLS correlated with VT arrhythmias (p = 0.004). NTproBNP predicted MACE (AUC 0.750, p = 0.03). SVGLS also predicted MACE (AUC 0.745, p = 0.03. In multivariate analysis, SVGLS and logNTproBNP maintained their predictive value (p = 0.004 and p = 0.009, respectively) Conclusion: In ACHD patients, SV GLS was found to predict MACE independently from NTproBNP and correlated with VT. Gal-3 correlated with NTproBNP and SVGLS as well as SVT and VT, but has not been shown to bear significant prognostic potential.
Report of Positive Placental Swabs for SARS-CoV-2 in an Asymptomatic Pregnant Woman with COVID-19
Authors: Ferraiolo Antonella , Barra Fabio , Kratochwila Chiara , Paudice Michele , Vellone Valerio Gaetano , Godano Elisabetta , Varesano Serena , Noberasco Giovanni , Ferrero Simone , Arioni Cesare
Published: June 2020

Currently, limited data on maternal and neonatal outcomes of pregnant women with infection and pneumonia related to SARS coronavirus 2 (SARS-CoV-2) are available. Our report aims to describe a case of placental swabs positive for the molecular research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 RNA in an asymptomatic woman with positive rhino-pharyngeal swab for SARS-CoV-2 who underwent an urgent cesarean section in our obstetrics unit. Sample collection, processing, and laboratory testing were conducted in accordance with the World Health Organization (WHO) guidance. In the next months, conclusive data on obstetrical outcomes concerning the gestational age and pregnancy comorbidity as well as the eventual maternal–fetal transmission are needed.
Rates of Intracranial Hemorrhage in Mild Head Trauma Patients Presenting to Emergency Department and Their Management: A Comparison of Direct Oral Anticoagulant Drugs with Vitamin K Antagonists
Authors: Savioli Gabriele , Ceresa Iride Francesca , Luzzi Sabino , Gragnaniello Cristian , Giotta Lucifero Alice , Del Maestro Mattia , Marasco Stefano , Manzoni Federica , Ciceri Luca , Gelfi Elia , Ricevuti Giovanni , Bressan Maria Antonietta
Published: June 2020

Background and objectives: Anticoagulants are thought to increase the risks of traumatic intracranial injury and poor clinical outcomes after blunt head trauma. The safety of using direct oral anticoagulants (DOACs) compared to vitamin K antagonists (VKAs) after intracranial hemorrhage (ICH) is unclear. This study aims to compare the incidence of post-traumatic ICH following mild head injury (MHI) and to assess the need for surgery, mortality rates, emergency department (ED) revisit rates, and the volume of ICH. Materials and Methods: This is a retrospective, single-center observational study on all patients admitted to our emergency department for mild head trauma from 1 January 2016, to 31 December 2018. We enrolled 234 anticoagulated patients, of which 156 were on VKAs and 78 on DOACs. Patients underwent computed tomography (CT) scans on arrival (T0) and after 24 h (T24). The control group consisted of patients not taking anticoagulants, had no clotting disorders, and who reported an MHI in the same period. About 54% in the control group had CTs performed. Results: The anticoagulated groups were comparable in baseline parameters. Patients on VKA developed ICH more frequently than patients on DOACs and the control group at 17%, 5.13%, and 7.5%, respectively. No significant difference between the two groups was noted in terms of surgery, intrahospital mortality rates, ED revisit rates, and the volume of ICH. Conclusions: Patients with mild head trauma on DOAC therapy had a similar prevalence of ICH to that of the control group. Meanwhile, patients on VKA therapy had about twice the ICH prevalence than that on the control group or patients on DOAC, which remained after correcting for age. No significant difference in the need for surgery was determined; however, this result must take into account the very small number of patients needing surgery.
Influence of Thermal Processing Factors, Linked to the Destabilisation of Austenite, on the Microstructural Variation of a White Cast Iron Containing 25% Cr and 0.6% Mo
Authors: González-Pociño Alejandro , Alvarez-Antolin Florentino , Asensio-Lozano Juan , Alvarez-Perez Hugo
Published: June 2020

Hypoeutectic white cast irons containing 25% Cr are used in ore-processing industries due to their high resistance to erosive wear. Applying a Design of Experiments (DoE), the aim of this study is to analyse the influence of thermal processing factors on the microstructural variation of a white cast iron containing 25% Cr and 0.6% Mo. The carbides present in the as-cast state are of the M7C3, M2C, and M3C types. M2C carbides precipitate on the eutectic M7C3 carbides favoured by heterogeneous nucleation conditions. Two kinetics compete during the destabilisation of austenite. One dissolves those eutectic carbides precipitated as a result of non-equilibrium solidification (M7C3 and M2C), while the other enables the precipitation of secondary M7C3 and M23C6 carbides. The M7C3 carbides begin to precipitate first. Low destabilisation temperatures and short dwell times are insufficient to dissolve the precipitated eutectic carbides from non-equilibrium conditions, thus favouring the presence of M2C carbides, which are associated with Mo. The factor that has the greatest influence on hardness is the tempering temperature. The optimal tempering temperature is found to be 500 °C. Short tempering times maintain the distortion of the ferrite unit cell. The precipitation of Cr carbides during tempering requires a temperature of 500 °C and the prior dissolution of the carbon precipitated during the initial stages of said tempering. With short tempering times, the Cr atoms still remain dissolved in the ferrite, distorting its unit cell and increasing the hardness of the matrix constituent of the alloy.
Decision Aids in Online Review Portals: An Empirical Study Investigating Their Effectiveness in the Sensemaking Process of Online Information Consumers
Authors: Ponathil Amal , Gramopadhye Anand , Chalil Madathil Kapil
Published: June 2020

There is an increasing concern about the trustworthiness of online reviews as there is no editorial process for verification of their authenticity. This study investigated the decision-making process of online consumers when reacting to a review, with the reputation score of the reviewer and the number of previous reviews incorporated along with anonymous and non-anonymous reviews. It recruited 200 participants and developed a 3 × 2 × 2 × 2 × 2 mixed experimental study, with the independent variables being the reaction to a review of a restaurant at 3 levels, the reputation score at 2 levels, the number of previous reviews at 2 levels, the valence of the reviews at 2 levels, and the level of anonymity at 2 levels. Five dependent variables were analyzed: level of trust, likelihood of going to the restaurant, a choice question of whether to go to the restaurant, confidence in the decision and the NASA-TLX workload. This study found that the reputation scores complemented the reaction to a review, improving the trust in the information and confidence in the decision made. The findings suggest that incorporating a user rating scale such as the reputation score of a user deters people from writing false or biased reviews and helps improve their accuracy. Although no significant effect of level of anonymity was found in this study, additional personal information about the users writing the review such as photos or other social media links may make a significant difference in the decision-making process.