bluuebunny/crossref_metadata_2025 huggingface dataset:
Oral administration of heat-inactivated Escherichia coli during suckling alleviated Salmonella typhimurium-derived intestinal injury after rat weaning
Authors: Cui Minghui , Tang Guangfu , Yan Fang , Wang Shunshan , Wang Xi , Yao Junhu , Xu Xiurong
Published: April 2023

Introduction

Newly weaned animals are susceptible to a wide range of microbial infections taking a high risk of developing post-weaning diarrhea. Trained immunity is the capacity of the innate immune system to produce a stronger and non-specific response against a secondary infection after the inflammatory response caused by previous stimulus has returned to normal state. The objective of this study was to evaluate if the heat-inactivated Escherichia coli (IEC) as an immunostimulant on suckling pups elicits a protective effect on the intestine of post-weaning rats challenged with Salmonella Typhimurium (S.Typhimurium). We adapted a newborn rat model for this purpose.

Methods

Sixty newborn pups were randomly separated into two groups: IEC group (n =30) orally administrated IEC during suckling, while the CON group received orally the same dose of saline. Both of the two group challenged with various doses of S.Typhimurium after experiencing a 4-week resting period. Twelve of individuals were selected to detect the survival rate, and ten of the rest were necropsied 48 hours post-challenge.

Results and Discussion

The results showed that oral administration of IEC during suckling alleviated the injury in ileal morphology induced by post-weaning S.Typhimurium infection via increasing the levels of two tight junction proteins [zonula occluden-1 (ZO-1) and Occludin-1] and several secreted proteins (Lysozyme, Mucin-2, and SIgA) in the intestinal mucosa. Furthermore, the pre-stimulation with IEC significantly increased cytokines tumor necrosis factor-alpha (TNF- α) and interleukin-1 beta (IL-1 β) expressions in an enhanced secondary reaction way after experiencing a 4-week resting period. This implicated the possible involvement of trained immunity. The 16S rDNA sequence results showed that pre-stimulation with IEC decreased the abundance of Clostridia, Prevotella, Christensenellaceae_R-7_group and Parabacteroides after intestinal infection of S.Typhimurium. Our results confirmed that the previous oral administration of IEC had a protective effect on S.Typhimurium-induced intestinal injury in weaned rats by inducing a robust immune response. The present study suggested a new strategy for preventing intestinal infection of newborn animals.
The prevalence of symptoms and its correlation with sex in polish COVID-19 adult patients: Cross-sectional online open survey
Authors: Lewek Pawel , Banaś Izabela , Witkowski Konrad , Lewek Joanna , Kardas Przemyslaw
Published: April 2023

Background

The understanding and treatment of COVID-19 has improved rapidly since December 2019 when SARS-CoV-2 was sequenced. However most papers on its symptomatology focus on hospitalized patients and address only a limited number of major presentations. Although differences depending on sex of COVID-19 patients have been previously confirmed (higher ICU admission and higher death rate for men), no publication has focused on sex-related differences in COVID-19 symptomatology.

Objective

The aim of the study was to present a reliable list of COVID-19 symptoms and identify any differences in symptom prevalence depending on sex.

Methods

A sample of Polish patients suffering from COVID-19 were surveyed using a cross-sectional anonymous online survey in Polish available on a web-based surveying platform (Survey Monkey). The survey included 20 questions asking about COVID-19 symptoms, days of occurrence (from day 1 until day 14 and “15 days or more”) and patient characteristics including sex, age, height, weight, place of residence and type of therapy received during COVID-19. The survey was made available during the third COVID-19 wave in Poland. The link to the survey was distributed across social networks. Participation was open to anyone willing, without any incentives. The data was analyzed statistically.

Results

Survey responses were collected from 2,408 participants (56.9% women) aged 18–90 (42 ± 12), 84.7% living in cities, who took part in the study between December 2020 and February 2021. Out of 54 predefined symptoms, the three most prevalent were fatigue (reported by 87.61% respondents), anosmia (73.74%) and headache (69.89%). Women were found to be more symptomatic than men, 31 symptoms occurred more often in women (including anosmia, headache and myalgias, p < 0.05). Subfebrility, fever and hemoptysis were more prevalent in men. Twelve symptoms (incl. hypothermia, sneezing and nausea) lasted longer in women than men (p < 0.05). Fatigue, cough, nasal dryness, xerostomia and polydipsia were the longest lasting symptoms of COVID-19 (lasted over 14 days).

Conclusion

Our study presents a wide range of symptoms, which may enable better recognition of COVID-19, especially in an outpatient setting. Understanding these differences in the symptomatology of community and hospitalized patients may help diagnose and treat patients faster and more accurately. Our findings also confirmed differences in symptomatology of COVID-19 between men and women, which may lay the foundation for a better understanding of the different courses of this disease in the sexes. Further studies are necessary to understand whether a different presentation correlates with a different outcome.
Comparison of the application of high-flow nasal oxygen with two different oxygen concentrations in infant and child laryngotracheal surgery
Authors: Liu Jianxia , Xiong Ling , Li Haisu , Du Min , Ru Xue , Xu Ying
Published: April 2023

Background

This study aimed to compare the use of the STRIVE Hi technique with 70 and 100% oxygen concentrations in children with 1st or 2nd degree laryngeal obstruction undergoing suspension laryngoscopic surgery.

Methods

Children aged 1 month to 6 years scheduled for suspension laryngoscopic surgery with spontaneous respiration were randomly divided into the 70% oxygen concentration group (HFNO70% group) and the 100% oxygen concentration group (HFNO100% group). The data recorded for all the patients included age and sex, comorbidities, preoperative physiological status, methods of induction and maintenance of anesthesia, course of the disease and surgical options, and duration of operation. The primary endpoint was the lowest oxygen saturations during the surgery. The secondary endpoints included the partial pressure of oxygen PaO2, the arterial pressure of carbon dioxide PaCO2, the peak transcutaneous carbon dioxide PtcCO2, and the incidence of desaturation (defined as SpO2 < 90%) or hypercarbia (PtcCO2 > 65 mmHg).

Results

A total of 80 children with 1st or 2nd degree laryngeal obstruction were included in the analysis. The median [IQR (range)] duration of spontaneous ventilation using STRIVE Hi was 52.5 [40–60 (30–170)]min and 62.5 [45–81 (20–200)]min in the HFNO 70% and HFNO 100% groups, respectively (p = 0.99); the lowest oxygen saturation recorded during the operation was 97.8 ± 2.1% and 96.8 ± 2.5%, respectively (p = 0.053); the mean PaO2 at the end of surgery was 184.6 ± 56.3 mmHg and 315.2 ± 101.3 mmHg, respectively (p < 0.001); and the peak transcutaneous CO2 was 58.0 ± 13.0 mmHg and 60.4 ± 10.9 mmHg, respectively (p = 0.373), despite a long operation time.

Conclusion

STRIVE Hi had a positive effect on children undergoing tubeless laryngeal surgery with spontaneous ventilation, and for children with 1st or 2nd degree laryngeal obstruction, there was no significant difference in maintaining the intraoperative oxygenation between the 70 and 100% oxygen concentration groups. The 100% oxygen concentration group showed significant hyperoxia, which has been proven to be associated with multiple organ damage. Using a relatively lower oxygen concentration of 70% can effectively reduce the hazards associated with hyperoxia compared to 100% oxygen concentration.

Clinical trial registration

[www.chictr.org.cn], identifier [CHICTR2200064500].
QuNex—An integrative platform for reproducible neuroimaging analytics
Authors: Ji Jie Lisa , Demšar Jure , Fonteneau Clara , Tamayo Zailyn , Pan Lining , Kraljič Aleksij , Matkovič Andraž , Purg Nina , Helmer Markus , Warrington Shaun , Winkler Anderson , Zerbi Valerio , Coalson Timothy S. , Glasser Matthew F. , Harms Michael P. , Sotiropoulos Stamatios N. , Murray John D. , Anticevic Alan , Repovš Grega
Published: April 2023

Introduction

Neuroimaging technology has experienced explosive growth and transformed the study of neural mechanisms across health and disease. However, given the diversity of sophisticated tools for handling neuroimaging data, the field faces challenges in method integration, particularly across multiple modalities and species. Specifically, researchers often have to rely on siloed approaches which limit reproducibility, with idiosyncratic data organization and limited software interoperability.

Methods

To address these challenges, we have developed Quantitative Neuroimaging Environment & Toolbox (QuNex), a platform for consistent end-to-end processing and analytics. QuNex provides several novel functionalities for neuroimaging analyses, including a “turnkey” command for the reproducible deployment of custom workflows, from onboarding raw data to generating analytic features.

Results

The platform enables interoperable integration of multi-modal, community-developed neuroimaging software through an extension framework with a software development kit (SDK) for seamless integration of community tools. Critically, it supports high-throughput, parallel processing in high-performance compute environments, either locally or in the cloud. Notably, QuNex has successfully processed over 10,000 scans across neuroimaging consortia, including multiple clinical datasets. Moreover, QuNex enables integration of human and non-human workflows via a cohesive translational platform.

Discussion

Collectively, this effort stands to significantly impact neuroimaging method integration across acquisition approaches, pipelines, datasets, computational environments, and species. Building on this platform will enable more rapid, scalable, and reproducible impact of neuroimaging technology across health and disease.
Whole-food plant-based Jumpstart for a Deaf and Hard of Hearing cohort
Authors: Friedman Susan M. , Scheuer Kim , Beha Beth Garver , Dewhirst Maria , Barnett Ted D.
Published: April 2023

Deaf and Hard of Hearing (DHH) patients are at high risk of developing chronic illness, and when they do, are at higher risk of poor outcomes than in a hearing community. Rochester Lifestyle Medicine Institute adapted its online, Zoom-based, medically-facilitated 15 Day Whole-Food Plant-Based (WFPB) Jumpstart program, to give DHH participants knowledge, skills, and support to make dietary changes to improve their health. Adaptations included having a medical provider present who is fluent in American Sign Language (ASL), is board-certified in Lifestyle Medicine, and has a Master of Science in Deaf Education; spotlighting participants when asking a question during the Q&A session; using ASL interpreters; utilizing closed captioning/automatic transcription during all Zoom meetings; and employing a Success Specialist to provide outreach via text and email throughout the program. Participants had significant positive changes in their eating pattern. They reported improvements in biometric measures as well as in how they were feeling. They all reported that they planned to continue to eat a more WFPB diet than they did prior to Jumpstart. All either agreed or strongly agreed that they learned important information, were confident that they knew the best eating pattern for health, and gained the skills they needed to make changes. Although this was a small pilot program, it suggests that this model can be used to provide education and support for behavior change that will lead to improved health in a DHH community.
Association of elevated plasma CCL5 levels with high risk for tic disorders in children
Authors: You Hai-zhen , Zhang Jie , Du Yaning , Yu Ping-bo , Li Lei , Xie Jing , Mi Yunhui , Hou Zhaoyuan , Yang Xiao-Dong , Sun Ke-Xing
Published: April 2023

Abnormal levels of some peripheral cytokines have been reported in children patients with tic disorders (TDs), but none of these cytokines can be a biomarker for this disease. Our aim was to systemically profile differentially expressed cytokines (DECs) in the blood of TD patients, examine their associations with TD development, and identify from them potential biomarkers for the prediction and management of the risk for TDs. In this study, a cytokine array capable of measuring 105 cytokines was used to screen for DECs in the plasma from 53 comorbidity-free and drug-naïve TD patients and 37 age-matched healthy controls. DECs were verified by ELISA and their associations with TD development were evaluated by binary logistic regression analysis. Elevation of a set of cytokines was observed in TD patients compared with controls, including previously uncharacterized cytokines in tic disorders, CCL5, Serpin E1, Thrombospondin-1, MIF, PDGF-AA, and PDGF-AB/BB. Further analysis of DECs revealed a significant association of elevated CCL5 with TD development (p = 0.005) and a significant ROC curve for CCL5 as a risk factor [AUC, 0.801 (95% CI: 0.707–0.895), p < 0.0001].

Conclusion

This study identifies associations of a set of circulating cytokines, particularly CCL5 with TD development, and provides evidence that high blood CCL5 has potential to be a risk factor for TD development.

Clinical Trial Registration

identifier ChiCTR-2000029616.
Effects of anaerobic exercise training on human function based on multiple linear regression
Authors: Xiao Haiting , Peng Kun , Sun Liman , Pan Feng
Published: April 2023

In recent years, with the continuous enhancement of people’s fitness awareness, more and more people are doing anaerobic exercise training. Although anaerobic exercise training can improve physical fitness, people also have some problems in anaerobic exercise training due to the lack of necessary exercise training guidance. Therefore, in order to give full play to the role of anaerobic exercise training and enhance human body functions, this paper studies the effects of anaerobic exercise training and anaerobic exercise training on human body functions based on multiple linear regression analysis (MLR). This paper proposes an anaerobic exercise training model based on MLR, and conducts research experiments from seven aspects including exercise capacity, immunity, and cardiovascular function. The experimental results show that the MLR-based anaerobic exercise training mode can improve exercise capacity by 5.48%, immunity by 3.08%, and cardiovascular function by 4.31%.
Severity related neuroanatomical and spontaneous functional activity alteration in adolescents with major depressive disorder
Authors: Zhang Xiaoliu , Cao Jun , Huang Qian , Hong Su , Dai Linqi , Chen Xiaorong , Chen Jianmei , Ai Ming , Gan Yao , He Jinglan , Kuang Li
Published: April 2023

Background

Major depressive disorder (MDD) is a disabling and severe psychiatric disorder with a high rate of prevalence, and adolescence is one of the most probable periods for the first onset. The neurobiological mechanism underlying the adolescent MDD remains unexplored.

Methods

In this study, we examined the cortical and subcortical alterations of neuroanatomical structures and spontaneous functional activation in 50 unmedicated adolescents with MDD vs. 39 healthy controls through the combined structural and resting-state functional magnetic resonance imaging.

Results

Significantly altered regional gray matter volume was found at broader frontal-temporal-parietal and subcortical brain areas involved with various forms of information processing in adolescent MDD. Specifically, the increased GM volume at the left paracentral lobule and right supplementary motor cortex was significantly correlated with depression severity in adolescent MDD. Furthermore, lower cortical thickness at brain areas responsible for visual and auditory processing as well as motor movements was found in adolescent MDD. The lower cortical thickness at the superior premotor subdivision was positively correlated with the course of the disease. Moreover, higher spontaneous neuronal activity was found at the anterior cingulum and medial prefrontal cortex, and this hyperactivity was also negatively correlated with the course of the disease. It potentially reflected the rumination, impaired concentration, and physiological arousal in adolescent MDD.

Conclusion

The abnormal structural and functional findings at cortico-subcortical areas implied the dysfunctional cognitive control and emotional regulations in adolescent depression. The findings might help elaborate the underlying neural mechanisms of MDD in adolescents.
Risky sexual practice and associated factors among street children of Wonago town, Gedeo zone, Southern Ethiopia
Authors: Zenebe Getachew Assefa , Alemu Wagaye , Muche Temesgen , Debela Berhanu Gidisa
Published: April 2023

Introduction

A “risky sexual practice” is any sexual act that might put an individual’s social, physical, and psychological health at risk and increase the likelihood of adverse sexual and reproductive health consequences. Street children have risky sexual practices, which place them at all kinds of health risks. Therefore, the aim of this study is to assess risky sexual practices and associated factors among street children in Wonago Town, southern Ethiopia.

Methods

A community-based cross-sectional study design was employed. About 214 street children, aged 10–18, residing in Wonago Town from September 1–30, 2021, participated in the study. Data was gathered from all of the street children in Wonago town. A pre-tested and structured interviewer-administered questionnaire was used to collect data. Epi data software was used to enter the collected data, which was then exported to SPSS for analysis. A logistic regression model was applied to identify the associated factors. A p value <0.05 was used to declare the significant variables.

Results

A total of 214 street children were involved in the study. Overall, the magnitude of risky sexual practices was 43.46 percent (95% CI: 38.3–48.62). About 52.7% of participants had ever had sexual intercourse in the last year, and of them, around 43.5% had more than one sexual partner. Among sexually active street children, 40.9% never used a condom during sexual intercourse. Age (Adjusted Odd Ratio (AOR): 1.42, 95%CI: 1.03–2.37), educational status (AOR: 5.73, 95%CI: 1.49–10.51), substance use (AOR: 1.24, 95%CI: 1.03–2.07), duration on the street (AOR: 2.14, 95%CI: 1.03–4.12), and daily income (AOR: 0.68, 95%CI: 0.32–0.98) were found to be significantly associated with risky sexual practices.

Conclusion

Risky sexual practices among street children were more prevalent in Wonago Town, Southern Ethiopia. Children’s age, educational status, daily income, duration on the street, and status of substance use were the factors that contributed to risky sexual practices. As a result, conducting information and education campaigns, developing income-generating activities for children, increasing children’s school enrollment and attendance, improving access to sexual and reproductive health services, and providing capacity-building training for health care providers may all help to reduce risky sexual practices.
Effects of multimorbidity coexistence on the risk of mortality in the older adult population in China
Authors: Su Zhili , Huang Li , Zhu Jinghui , Cui Shichen
Published: April 2023

Background

Multimorbidity coexistence is a serious public health issue affecting a significant number of older adults worldwide. However, associations between multimorbidity and mortality are rarely studied in China. We assessed the effects of multimorbidity coexistence on mortality among a nationwide sample of older adults from China.

Objective

We analyzed 10-year (2008–2018) longitudinal data of 12,337 individuals who took part in China, a nationwide survey of people aged 65 years and above. We used the Cox proportional hazard model to determine the effects of multimorbidity on the all-cause mortality risk. We also examined mortality risk between sex and age obtained through differential analysis.

Results

At baseline, 30.2, 29.9, and 39.9% of participants had 0, 1, and 2 or more diseases, respectively. The cumulative follow-up of this study was 27,428 person-years (median follow-up = 2.7 years; range, 0.01–11.3 years), with 8297 deaths. The HRs (95% CIs) for all-cause mortality in participants with 1, and 2 or more conditions compared with those with none were 1.04 (0.98, 1.10) and 1.12 (1.06, 1.18), respectively. The heterogeneity analysis indicated that, the mortality risk for 80–94 years and 95–104 years group with multimorbidity coexistence is 1.12 (1.05–1.21) and 1.11 (1.01–1.23), respectively, but the mortality risk for 65–79 years group with multimorbidity coexistence was not statistically significant. The heterogeneity analysis indicated that, the mortality risk for men and women in older adults with multimorbidity coexistence is 1.15 (1.06, 1.25) and 1.08 (1.01, 1.17), respectively.

Conclusion

Multimorbidity coexistence is associated with an increase in an increased risk of death in older individuals, with the effect being relatively significant in those aged 80–94 years.
Identification of pseudo-immune tolerance for chronic hepatitis B patients: Development and validation of a non-invasive prediction model
Authors: Li Shuo , Li Zhiguo , Du Hongbo , Zao Xiaobin , Gan Da’nan , Yang Xianzhao , Li Xiaoke , Xing Yufeng , Ye Yong’an
Published: April 2023

Background and aims

Patients with chronic hepatitis B (CHB) in the immune tolerant (IT) phase were previously thought to have no or slight inflammation or fibrosis in the liver. In fact, some CHB patients with normal ALT levels still experience liver fibrosis. This study aimed to develop and validate a non-invasive model for identifying pseudo-immune tolerance (pseudo-IT) of CHB by predicting significant liver fibrosis.

Methods

This multi-center study enrolled a total of 445 IT-phase patients who had undergone liver biopsy for the training cohort (n = 289) and validation cohort (n = 156) during different time periods. A risk model (IT-3) for predicting significant liver fibrosis (Ishak score ≥ 3) was developed using high-risk factors which were identified using multivariate stepwise logistic regression. Next, an online dynamic nomogram was created for the clinical usage. The receiver operating characteristic (ROC) curve, net reclassification improvement and integrated discrimination improvement were used to assess the discrimination of the IT-3 model. Calibration curves were used to evaluate the models’ calibration. The clinical practicability of the model was evaluated using decision curve analysis and clinical impact curves.

Results

8.8% (39 of 445) patients presented with significant liver fibrosis in this study. Aspartate aminotransferase (AST), hepatitis B e-antigen (HBeAg), and platelet (PLT) were included in the prediction model (IT-3). The IT-3 model showed good calibration and discrimination both in the training and validation cohorts (AUC = 0.888 and 0.833, respectively). The continuous NRI and IDI showed that the IT-3 model had better predictive accuracy than GPR, APRI, and FIB-4 (p < 0.001). Decision curve analysis and clinical impact curves were used to demonstrate the clinical usefulness. At a cut-off value of 106 points, the sensitivity and specificity were 91.7 and 70.2%, respectively.

Conclusion

The IT-3 model proved an accurate non-invasive method in identifying pseudo-IT of CHB, which can help to formulate more appropriate treatment strategies.
High-Throughput Microscopy Analysis of Mitochondrial Membrane Potential in 2D and 3D Models
Authors: Vianello Caterina , Dal Bello Federica , Shin Sang Hun , Schiavon Sara , Bean Camilla , Magalhães Rebelo Ana Paula , Knedlík Tomáš , Esfahani Emad Norouzi , Costiniti Veronica , Lacruz Rodrigo S. , Covello Giuseppina , Munari Fabio , Scolaro Tommaso , Viola Antonella , Rampazzo Elena , Persano Luca , Zumerle Sara , Scorrano Luca , Gianelle Alessio , Giacomello Marta
Published: April 2023

Recent proteomic, metabolomic, and transcriptomic studies have highlighted a connection between changes in mitochondria physiology and cellular pathophysiological mechanisms. Secondary assays to assess the function of these organelles appear fundamental to validate these -omics findings. Although mitochondrial membrane potential is widely recognized as an indicator of mitochondrial activity, high-content imaging-based approaches coupled to multiparametric to measure it have not been established yet. In this paper, we describe a methodology for the unbiased high-throughput quantification of mitochondrial membrane potential in vitro, which is suitable for 2D to 3D models. We successfully used our method to analyze mitochondrial membrane potential in monolayers of human fibroblasts, neural stem cells, spheroids, and isolated muscle fibers. Moreover, by combining automated image analysis and machine learning, we were able to discriminate melanoma cells from macrophages in co-culture and to analyze the subpopulations separately. Our data demonstrated that our method is a widely applicable strategy for large-scale profiling of mitochondrial activity.
Method for Intensive Gas–Liquid Dispersion in a Stirred Tank
Authors: Voinov Nikolai A. , Frolov Alexander S. , Bogatkova Anastasiya V. , Zemtsov Denis A. , Zhukova Olga P.
Published: April 2023

This article presents the results of hydrodynamics and mass exchange in a stirred tank upon the introduction of gas from an open gas vortex cavity into local liquid regions with reduced pressure. It establishes conditions for the intensive dispersion of gas. Velocity fields and liquid pressure behind the stirrer paddles are determined by numerical simulation in OpenFOAM. The gas content value, gas bubble diameters, and phase surface are determined experimentally. The stirrer power criterion is calculated by taking into account the gas content and power input. The experimental mass transfer data based on the absorption of atmospheric oxygen into water during the dispersion of gas from the open vortex cavity in the local liquid regions behind the rotating stirrer paddles are presented. In this case, the energy dissipation from the rotating stirrer reaches 25 W/kg, with a phase surface of 1000 m−1 and a surface mass transfer coefficient of up to 0.3·10−3 m/s. These parameters are obviously higher than the data obtained in the apparatus for mass exchange through surface vorticity. The advantage of the given method for gas dispersion in a liquid is the functional stability of the apparatus regardless of how deep the stirrer is immersed in the liquid or the temperature or pressure of the gas. Apparatuses based on the intensive gas dispersion method allow for varying the mass transfer coefficient and gas content across a broad range of values. This allows establishing a dependency between the experimentally obtained mass transfer coefficient, energy dissipation, and phase surface values. An equation for calculating the mass transfer coefficient is formulated by taking into account the geometric parameters of the stirrer apparatus based on the stirring power and phase surface values.
Continuous Flow Separation of Red Blood Cells and Platelets in a Y-Microfluidic Channel Device with Saw-Tooth Profile Electrodes via Low Voltage Dielectrophoresis
Authors: Hewlin Rodward L. , Edwards Maegan
Published: April 2023

Cell counting and sorting is a vital step in the purification process within the area of biomedical research. It has been widely reported and accepted that the use of hydrodynamic focusing in conjunction with the application of a dielectrophoretic (DEP) force allows efficient separation of biological entities such as platelets from red blood cell (RBC) samples due to their size difference. This paper presents computational results of a multiphysics simulation modelling study on evaluating continuous separation of RBCs and platelets in a microfluidic device design with saw-tooth profile electrodes via DEP. The theoretical cell particle trajectory, particle cell counting, and particle separation distance study results reported in this work were predicted using COMSOL v6.0 Multiphysics simulation software. To validate the numerical model used in this work for the reported device design, we first developed a simple y-channel microfluidic device with square “in fluid” electrodes similar to the design reported previously in other works. We then compared the obtained simulation results for the simple y-channel device with the square in fluid electrodes to the reported experimental work done on this simple design which resulted in 98% agreement. The design reported in this work is an improvement over existing designs in that it can perform rapid separation of RBCs (estimated 99% purification) and platelets in a total time of 6–7 s at a minimum voltage setting of 1 V and at a minimum frequency of 1 Hz. The threshold for efficient separation of cells ends at 1000 kHz for a 1 V setting. The saw-tooth electrode profile appears to be an improvement over existing designs in that the sharp corners reduced the required horizontal distance needed for separation to occur and contributed to a non-uniform DEP electric field. The results of this simulation study further suggest that this DEP separation technique may potentially be applied to improve the efficiency of separation processes of biological sample scenarios and simultaneously increase the accuracy of diagnostic processes via cell counting and sorting.
Choroidal Hyperreflective Nodules Detected by Infrared Reflectance Images Are a Diagnostic Criterion for Neurofibromatosis Type 1 Patients Excluding Those with High Myopia
Authors: de Rivas Marta Orejudo , Gabás Javier Mateo , Cabeza Miguel Ángel Torralba , Floría Olivia Esteban , Latorre Raquel Herrero , Moscarda Eva Núñez , Clavería Julia Aramburu , Rivasés Guillermo Pérez , Puyuelo Javier Ascaso
Published: April 2023

Neurofibromatosis type 1 (NF1) is one of the central nervous system’s most common autosomal dominant conditions. The diagnosis is based on the clinical diagnostic criteria and/or a molecularly confirmed mutation in the NF1 gene. This study investigated the possibility of substantiating choroidal nodules as a diagnostic criterion for the disease, including patients affected with and without high myopia. A cross-sectional study was carried out in 60 eyes of 30 adult patients diagnosed with NF1. A total of 30 healthy individuals of equivalent age and sex served as control. The Spectralis HRA+OCT MultiColor (Heidelberg Engineering GmbH, Heidelberg, Germany) evaluated the presence of choroidal abnormalities with near-infrared reflectance imaging. Secondly, the presence of iridian Lisch nodules was evaluated by slit lamp examination. Near-infrared reflectance imaging showed the presence of choroidal hyperreflective nodules in 83% of the patients diagnosed with NF1, while these choroidal abnormalities were not observed in any control subject. The patients diagnosed with NF1 associated with high myopia were the only ones who did not present the characteristic choroidal disorders. Therefore, when excluding patients diagnosed with high myopia, choroidal nodules were more frequent than Lisch nodules in a statistically significant proportion. Hyperreflective nodules detected by near-infrared reflectance imaging are as regular as Lisch nodules or even significantly more frequent when excluding high myope patients. Our observation of the mutual exclusion of choroidal hyperreflective nodules and high myopia in the NF1 patients seems a novel and interesting remark.
Recent Case Reveals a Nineteenth Century Trauma Analysis and Presentation of a Skull as Evidence in a Homicide Trial
Authors: Garvin Heather , Hohulin Lindsey , VanBaarle Alexis , Wilson Andrew
Published: April 2023

Following a recent death, a human skull was found in a worn physician bag at the decedent’s residence. The bag was labeled with the name “G.B. Ward”, who was found to be a practicing physician and surgeon in the late 1800s, and contained historic medical paraphernalia and letters and receipts dated to the late 1890s. A forensic anthropological analysis of the skull concluded that it was not of modern medicolegal significance but revealed certain inconsistencies with standard professionally prepared anatomical specimens. This initiated further investigation into the history of G.B. Ward, which ultimately resulted in the circumstantial identification of the skull and revealed more than forty detailed newspaper accounts of its evidentiary use in an 1895 homicide trial in the rural United States. Ultimately, the Prosecution’s argument was likely based on the misinterpretation of a transmaxillary (Le Fort) fracture, which had yet to be defined in the medical literature. This case study emphasizes the importance of investigative and forensic anthropological efforts and cautions against cursory designations of skeletal remains as medical specimens. It also highlights the early recognition of the evidentiary value of skeletal remains in court cases and pioneering efforts in performing forensic skeletal trauma analyses.
The Temporary Mental Nerve Paresthesia as an Outcome of Dentigerous Cyst Removal during Preparation for Dental Implant Placement: A Case Report
Authors: Nelke Kamil , Janeczek Maciej , Pasicka Edyta , Żak Krzysztof , Łukaszewski Marceli , Jadach Radosław , Dobrzyński Maciej
Published: April 2023

The usage of xenograft material is widely used in almost all oral cavity bone defects for regenerative and reconstructive purposes. The presented xenograft usage in the following care report enabled good bone defect healing and enabled the preservation of affected premolars. It is quite common to use any possible variations of bone materials to ensure bone defect improved healing. In some cases, the scope of surgeries requires the removal of each cyst within close proximity to various nerves and vessels. The inferior alveolar, infraorbital, lingual, and mental nerves are those most commonly adjacent to most operating sites in jaw bones. The usage of some additional materials such as collagen sponges, bone substitutes, resorbable membranes, or other additional materials are useful in each bone defect reconstruction but should be handled with care, as described in the following case. Before planning their usage, it is important to perform each surgery with close cone beam computed tomography imaging, which is very helpful to establish the scope of each lesion and the proximity of vital structures. There are a lot of factors that might influence any possible nerve damage, especially the different nerve anatomical variations. Even factors including the subperiosteal preparation and compression of adjacent tissues might influence later nerve function. When the lesion is expanding through the buccal cortical plate and when soft tissue fluctuation is present, some special care is needed. Similar to the presented case, a limitation in crushing, blowing, or any irritation of nerve fibers improves later postoperative outcomes. When the wound and surrounding tissues are handled with care, a limited possibility of any damage or paresthesia can occur. When the nerve itself is damaged or cut, loss of function can be permanent. Immediately after or even prophylactic prescription 1–2 days before the surgery of Vitamin B with NSAIDs (Non-steroidal anti-inflammatory drugs) (or other additional supplementary medicaments can improve nerve function in time. Possible nerve damage can be divided into many etiological factors. A quite different situation arises when the nerve is pulled in by the cyst growth into the cyst wall. The presented case report describes the outcomes of a cyst removal from the mandibular basis and treatment modalities.
Computational Protocol for the Identification of Candidates for Radioastronomical Detection and Its Application to the C3H3NO Family of Isomers
Authors: Alessandrini Silvia , Melosso Mattia , Rivilla Víctor M. , Bizzocchi Luca , Puzzarini Cristina
Published: April 2023

The C3H3NO family of isomers is relevant in astrochemistry, even though its members are still elusive in the interstellar medium. To identify the best candidate for astronomical detection within this family, we developed a new computational protocol based on the minimum-energy principle. This approach aims to identify the most stable isomer of the family and consists of three steps. The first step is an extensive investigation that characterizes the vast number of compounds having the C3H3NO chemical formula, employing density functional theory for this purpose. The second step is an energy refinement, which is used to select isomers and relies on coupled cluster theory. The last step is a structural improvement with a final energy refinement that provides improved energies and a large set of accurate spectroscopic parameters for all isomers lying within 30 kJ mol−1 above the most stable one. According to this protocol, vinylisocyanate is the most stable isomer, followed by oxazole, which is about 5 kJ mol−1 higher in energy. The other stable species are pyruvonitrile, cyanoacetaldehyde, and cyanovinylalcohol. For all of these species, new computed rotational and vibrational spectroscopic data are reported, which complement those already available in the literature or fill current gaps.
Photoelectrocatalytic Dioxygen Reduction Based on a Novel Thiophene-Functionalized Tricarbonylchloro(1,10-phenanthroline)rhenium(I)
Authors: Li Yu-Qin , Wang Ke-Zhi
Published: April 2023

A novel Re (I) complex of [Re(CO)3Cl(L)], {L = 2-([2,2’-bithiophen]-5-yl)-1-phenyl-1H-imidazo [4,5-f][1,10]phenanthroline}, was synthesized, and its optical (UV–Visible absorption and emission spectroscopy), cyclovoltammetric and photoelectrochemical oxygen reduction properties were studied. The geometric and electronic properties were also investigated by density functional theory calculations. It was found that the ITO electrode coated with drop-casted [Re(CO)3Cl(L)] film exhibited cathodic photocurrent generation characteristics. The illuminated film exhibited a maximum cathodic photocurrent up to 30.4 μA/cm2 with an illumination intensity of 100 mW/cm2 white light at a bias potential of −0.4 V vs. SCE in O2-saturated electrolyte solution, which was reduced by 5.1-fold when thoroughly deoxygenated electrolyte solution was used, signaling that the electrode performed well on the photoelectrochemical oxygen reduction. The photo-electrocatalytic hydrogen peroxide production was proved with a maximum H2O2 concentration of 6.39 μM during 5 h of the photoelectrocatalytic process. This work would guide the construction of more efficient rhenium-based photo(electro)catalytic molecular systems for O2 sensing, hydrogen peroxide production and other types of photoelectrochemical energy conversion and storage.
Ppb-Level Hydrogen Sulfide Gas Sensor Based on the Nanocomposite of MoS2 Octahedron/ZnO-Zn2SnO4 Nanoparticles
Authors: Wu Di , Akhtar Ali
Published: April 2023

Hydrogen sulfide (H2S) detection is extremely necessary due to its hazardous nature. Thus, the design of novel sensors to detect H2S gas at low temperatures is highly desirable. In this study, a series of nanocomposites based on MoS2 octahedrons and ZnO-Zn2SnO4 nanoparticles were synthesized through the hydrothermal method. Various characterizations such as X-ray diffraction (XRD), Brunauer–Emmett–Teller (BET), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectrum (XPS) have been used to verify the crystal phase, morphology and composition of synthesized nanocomposites. Three gas sensors based on the nanocomposites of pure ZnO-Zn2SnO4 (MS-ZNO-0), 5 wt% MoS2-ZnO-Zn2SnO4 (MS-ZNO-5) and 10 wt% MoS2-ZnO-Zn2SnO4 (MS-ZNO-10) were fabricated to check the gas sensing properties of various volatile organic compounds (VOCs). It showed that the gas sensor of (MS-ZNO-5) displayed the highest response of 4 to 2 ppm H2S and fewer responses to all other tested gases at 30 °C. The sensor of MS-ZNO-5 also displayed humble selectivity (1.6), good stability (35 days), promising reproducibility (5 cycles), rapid response/recovery times (10 s/6 s), a limit of detection (LOD) of 0.05 ppm H2S (Ra/Rg = 1.8) and an almost linear relationship between H2S concentration and response. Several elements such as the structure of MoS2, higher BET-specific surface area, n-n junction and improvement in oxygen species corresponded to improving response.
Preparation of Chiral Porous Organic Cage Clicked Chiral Stationary Phase for HPLC Enantioseparation
Authors: Gong Ya-Nan , Ma Qi-Yu , Wang Ying , Zhang Jun-Hui , Zhang You-Ping , Liang Rui-Xue , Wang Bang-Jin , Xie Sheng-Ming , Yuan Li-Ming
Published: April 2023

Porous organic cages (POCs) are a new subclass of porous materials, which are constructed from discrete cage molecules with permanent cavities via weak intermolecular forces. In this study, a novel chiral stationary phase (CSP) has been prepared by chemically binding a [4 + 6]-type chiral POC (C120H96N12O4) with thiol-functionalized silica gel using a thiol-ene click reaction and applied to HPLC separations. The column packed with this CSP presented good separation capability for chiral compounds and positional isomers. Thirteen racemates have been enantioseparated on this column, including alcohols, diols, ketones, amines, epoxides, and organic acids. Upon comparison with a previously reported chiral POC NC1-R-based column, commercial Chiralpak AD-H, and Chiralcel OD-H columns, this column is complementary to these three columns in terms of its enantiomeric separation; and can also separate some racemic compounds that cannot be separated by the three columns. In addition, eight positional isomers (iodoaniline, bromoaniline, chloroaniline, dibromobenzene, dichlorobenzene, toluidine, nitrobromobenzene, and nitroaniline) have also been separated. The influences of the injection weight and column temperature on separation have been explored. After the column has undergone multiple injections, the relative standard deviations (RSDs) for the retention time and selectivity were below 1.0 and 1.5%, respectively, indicating the good reproducibility and stability of the column for separation. This work demonstrates that POCs are promising materials for HPLC separation.
Conductive Polymer Nanoparticles as Solid Contact in Ion-Selective Electrodes Sensitive to Potassium Ions
Authors: Bao Hui , Ye Jin , Zhao Xuyan , Zhang Yuan
Published: April 2023

A preparation method of nanocomposites based on poly (3-octylthiophene-2,5-diyl) (POT) and carbon black (CB) as the transducer of an all-solid potassium ion selective electrode is proposed. POT is used as the dispersant of CB, and the obtained nanocomposites have unique characteristics, including high conductivity, high capacitance and high stability. The potassium ion selective electrode based on POT and CB was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronopotentiometry. The results showed that the detection limit of potassium ions was 10−6.2 M, and the slope was 57.6 ± 0.8 mV/façade. The water layer test and anti-interference test show that the electrode has high hydrophobicity, the static contact angle reaches 139.7° and is not easily affected by light, O2 and CO2.
Hydrogel-Film-Fabricated Fluorescent Biosensors with Aggregation-Induced Emission for Albumin Detection through the Real-Time Modulation of a Vortex Fluidic Device
Authors: Hu Qi , Luo Xuan , Tohl Damian , Pham Anh Tran Tam , Raston Colin , Tang Youhong
Published: April 2023

Hydrogels have various promising prospects as a successful platform for detecting biomarkers, and human serum albumin (HSA) is an important biomarker in the diagnosis of kidney diseases. However, the difficult-to-control passive diffusion kinetics of hydrogels is a major factor affecting detection performance. This study focuses on using hydrogels embedded with aggregation-induced emission (AIE) fluorescent probe TC426 to detect HSA in real time. The vortex fluidic device (VFD) technology is used as a rotation strategy to control the reaction kinetics and micromixing during measurement. The results show that the introduction of VFD could significantly accelerate its fluorescence response and effectively improve the diffusion coefficient, while VFD processing could regulate passive diffusion into active diffusion, offering a new method for future sensing research.
Acceleration of CRISPR/Cas9-Mediated Editing at Multiple Sites in the Saccharomyces cerevisiae Genome
Authors: Karpukhin Alexey D. , Sabirzyanov Fanis A. , Serebrianyi Vsevolod A.
Published: April 2023

The application of the CRISPR/Cas9-based genome editing technique to the yeast Saccharomyces cerevisiae has made it possible to simultaneously modify several sites, particularly to integrate several expression cassettes. The existing methods provide high efficiency for such modifications; however, common protocols include several preparatory steps, namely, the construction of an intermediate Cas9-expressing strain, the assembly of a plasmid bearing several single guide RNA (sgRNA) expression cassettes, and the surrounding integrated DNA fragments with long flanks for recombination with target loci. Since these preparatory steps are time consuming and may not be desirable in some types of experiments, we explored the possibility of multiple integration without these steps. We have demonstrated that it is possible to skip them simultaneously and integrate up to three expression cassettes into separate sites by transforming the recipient strain with the Cas9 expression plasmid, three differently marked sgRNA plasmids, and three donor DNAs flanked with short (70 bp) arms for recombination. This finding increases the flexibility of choosing the optimal experimental design for multiple editing of the genome of S. cerevisiae and can significantly accelerate such experiments.
Analysis of the Influence of Process and Formulation Properties on the Drying Behavior of Pharmaceutical Granules in a Semi-Continuous Fluid Bed Drying System
Authors: Vandeputte Tuur , Ghijs Michael , Peeters Michiel , De Man Alexander , Van Hauwermeiren Daan , Dos Santos Schultz Eduardo , Vigh Tamas , Stauffer Fanny , Nopens Ingmar , De Beer Thomas
Published: April 2023

In the last decade, twin-screw wet granulation became an essential technology for continuous pharmaceutical tablet production. Consequently, interest in (semi-)continuous fluidized bed drying systems as a subsequent processing unit has grown. In parallel, it has become pivotal to fully understand and control manufacturing processes in line with in the quality-by-design paradigm. Formulation-generic prediction models would enormously facilitate digitally enhanced process development and require dedicated experimental data collection and process knowledge. To obtain this knowledge, three experimental campaigns were performed in this work. Firstly, an investigation into the effect of dryer process settings on drying behavior is presented. Secondly, the effect of active pharmaceutical ingredient properties on drying was assessed by producing granules of similar particle size and porosity and evaluating their drying and breakage behavior. Finally, additional experiments with varying active pharmaceutical ingredients and drug load were conducted to increase the genericity of the data set. This knowledge can be used in mathematical process modelling.