bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Deadwood Decomposition on the Restoration of Soil Cover in Landslide Areas of the Karpaty Mountains, Poland
Authors: Piaszczyk Wojciech , Lasota Jarosław , Gaura Grzegorz , Błońska Ewa
Published: February 2021

Disturbances play an essential role in the shaping of temporal and spatial heterogeneity in natural community structures. The aim of this study was to provide an assessment of the deadwood influence on the chemical and biochemical properties of soils in a landslide area. The samples used to determine soil properties were collected from the entire landslide area, with locations distributed on a regular grid (50 × 50 m). The soil samples were collected from directly under the logs, and background soil samples were taken 1 m from the deadwood logs. The effect of the deadwood decomposition process was visible in the total organic carbon (C) and nitrogen (N) content and microbial activity of the soil. An increase in the enzyme activity and microbial biomass of the soil from directly beneath the deadwood was noted. In this study, it was found that a greater stock of deadwood was present in the accumulation zone, which resulted in a stronger effect of the released components on the soil cover. In order to restore landslide soils, microbial activity can be effectively stimulated by leaving deadwood on the landslide surface.
High Rainfall Inhibited Soil Respiration in an Asian Monsoon Forest in Taiwan
Authors: Yu Jui-Chu , Chiang Po-Neng , Lai Yen-Jen , Tsai Ming-Jer , Wang Ya-Nan
Published: February 2021

Soil respiration represents the second largest carbon flux, next to photosynthesis of the terrestrial biosphere, and thus plays a dual role in regional and global carbon cycles. However, soil respiration in Asian monsoon forests with high rainfall has rarely been studied. In this study, we continuously measured soil respiration using a 12-channel automated chamber system in a 61-year-old Japanese cedar forest in central Taiwan with annual rainfall greater than 2500 mm. A 4-year (2011–2014) continuous half-hourly dataset was used to quantify the influences of soil temperature and moisture, especially rainfall events, on both total soil respiration (Rs) and heterotrophic respiration (Rh). The annual mean Rs was approximately 10.8 t C ha−1 (ranging from 10.7 to 10.9) t C ha−1, with Rh contributing approximately 74.6% (ranging from 71.7% to 80.2%). Large seasonal variations in both Rs and Rh were primarily controlled by soil temperature. Over 45.8% of total annual rainfall amounts were provided by strong rainfall events (over 50 mm), and over 40% of rainfall events occurred during summers between 2012 and 2014. These strong rainfall events caused rainwater to enter soil pores and cover the soil surface, which resulted in limited soil microorganism activity and, consequently, restricted CO2 production. The mean Q10 values were 2.38 (ranging from 1.77 to 2.65) and 2.02 (ranging from 1.71 to 2.34) for Rs and Rh, respectively. The Q10 values in this study, which were lower than in global forest ecosystems, may imply that the interannual Rs values observed in this study that were caused by high rainfall were less temperature-dependent than the Rs levels in global forest ecosystems. Both Rs and Rh were negatively correlated with soil moisture, which indicated that the soil moisture levels in the studied forest were usually under saturated conditions. These results also provide the lack of data for respiration in the Asian monsoon region under high-rainfall conditions.
Timing of Contractional Tectonics in the Miocene Foreland Basin System of the Umbria Pre-Apennines (Italy): An Updated Overview
Authors: Brozzetti Francesco , Cirillo Daniele , Luchetti Lucina
Published: February 2021

A large dataset of lithostratigraphic and biostratigraphic data, concerning the Early-Late Miocene turbidite succession of the Umbria pre-Apennines, is presented and analyzed. The data come from the study of 24 sections that are representative of all the main tectonic units cropping out between the front of the Tuscan allochthon and the Umbria-Marche calcareous chain. The sections have been dated using quantitative calcareous nannofossil biostratigraphy and, wherever possible, they were correlated through key-beds recognition. Such a multidisciplinary approach allowed us to reconstruct the evolution of the Umbria foredeep over time and to unveil the chronology of compressive deformations by defining: (i) the onset of the foredeep stage in each structural unit, (ii) the age of depocenter-shifting from a unit to the adjacent one, (iii) the progressive deactivation of the western sector of the foredeep due to the emplacement of allochthon units, and (iv) the internal subdivisions of the basin due to the presence of foreland ramp faults or thrust-related structures. A further original outcome of our study is having brought to light the Late Burdigalian “out-of-sequence” reactivation of the Tuscan allochthon which bounded westward the foredeep, and the subsequent protracted period of tectonic stasis that preceded the deformations of the Umbrian parautochthon.
Quantifying the Impact of a Tsunami on Data-Driven Earthquake Relief Zone Planning in Los Angeles County via Multivariate Spatial Optimization
Authors: Gu Yueqi , Aydin Orhun , Sosa Jacqueline
Published: February 2021

Post-earthquake relief zone planning is a multidisciplinary optimization problem, which required delineating zones that seek to minimize the loss of life and property. In this study, we offer an end-to-end workflow to define relief zone suitability and equitable relief service zones for Los Angeles (LA) County. In particular, we address the impact of a tsunami in the study due to LA’s high spatial complexities in terms of clustering of population along the coastline, and a complicated inland fault system. We design data-driven earthquake relief zones with a wide variety of inputs, including geological features, population, and public safety. Data-driven zones were generated by solving the p-median problem with the Teitz–Bart algorithm without any a priori knowledge of optimal relief zones. We define the metrics to determine the optimal number of relief zones as a part of the proposed workflow. Finally, we measure the impacts of a tsunami in LA County by comparing data-driven relief zone maps for a case with a tsunami and a case without a tsunami. Our results show that the impact of the tsunami on the relief zones can extend up to 160 km inland from the study area.
Gold in Mineralized Volcanic Systems from the Lesser Khingan Range (Russian Far East): Textural Types, Composition and Possible Origins
Authors: Berdnikov Nikolai , Nevstruev Victor , Kepezhinskas Pavel , Astapov Ivan , Konovalova Natalia
Published: February 2021

While gold partitioning into hydrothermal fluids responsible for the formation of porphyry and epithermal deposits is currently well understood, its behavior during the differentiation of metal-rich silicate melts is still subject of an intense scientific debate. Typically, gold is scavenged into sulfides during crustal fractionation of sulfur-rich mafic to intermediate magmas and development of native forms and alloys of this important precious metal in igneous rocks and associated ores are still poorly documented. We present new data on gold (Cu-Ag-Au, Ni-Cu-Zn-Ag-Au, Ti-Cu-Ag-Au, Ag-Au) alloys from iron oxide deposits in the Lesser Khingan Range (LKR) of the Russian Far East. Gold alloy particles are from 10 to 100 µm in size and irregular to spherical in shape. Gold spherules were formed through silicate-metal liquid immiscibility and then injected into fissures surrounding the ascending melt column, or emplaced through a volcanic eruption. Presence of globular (occasionally with meniscus-like textures) Cu-O micro-inclusions in Cu-Ag-Au spherules confirms their crystallization from a metal melt via extremely fast cooling. Irregularly shaped Cu-Ag-Au particles were formed through hydrothermal alteration of gold-bearing volcanic rocks and ores. Association of primarily liquid Cu-Ag-Au spherules with iron-oxide mineralization in the LKR indicates possible involvement of silicate-metallic immiscibility and explosive volcanism in the formation of the Andean-type iron oxide gold-copper (IOCG) and related copper-gold porphyry deposits in the deeper parts of sub-volcanic epithermal systems. Thus, formation of gold alloys in deep roots of arc volcanoes may serve as a precursor and an exploration guide for high-grade epithermal gold mineralization at shallow structural levels of hydrothermal-volcanic environments in subduction zones.
When William Came: A Prophetic Propaganda War
Authors: Fukamachi Satoru
Published: February 2021

When William Came by Saki (H. H. Munro) is a unique novel in the genre of invasion literature. Starting after a fictional war between Britain and Germany, it depicts no scenes of invasion. Recently, there have been studies from the perspective of how Munro and other authors in the genre viewed Germany and Britain. Some studies also refer to Munro’s deliberate lack of depiction of the war. However, it seems that no studies have looked into the reasons why the war is not depicted. This paper argues that the story is not about showing British military unpreparedness but about how psychological weapons work. It could even be said that depictions of war would distract from the focus on propaganda and its effect on people. Considering this work as being about a British and German propaganda war opens up a new perspective that is different from previous studies. When William Came is a work that points out Britain’s unpreparedness for psychological war by imagining and detailing possible propaganda strategies. It has been said that the novel’s ending is unsatisfactory, as it only ends up showing the potential for youth resistance. However, if it is understood that this novel, from beginning to end, is about a propaganda battle, a war that is fought under the surface, then the final chapter can also be understood as a thrilling one.
Perceived Environmental Dynamism Promotes Entrepreneurial Team Member’s Innovation: Explanations Based on the Uncertainty Reduction Theory
Authors: Deng Xiao , Guo Xi , Wu Yenchun Jim , Chen Min
Published: February 2021

This study aims to examine the effect of perceived environmental dynamism on entrepreneurial team member’s innovation. Based on the uncertainty reduction theory, this study constructs a multilevel moderated mediation model of the relationship between perceived environmental dynamism and entrepreneurial team member’s innovation. By collecting questionnaires from 117 entrepreneurial team leaders and 479 team members in China, this research found that perceived environmental dynamism could stimulate entrepreneurial team members’ innovation via triggering their information exchange behavior. In addition, entrepreneurial team members’ intolerance for uncertainty and team cooperative climate can moderate the indirect positive relationship between perceived environmental dynamism and individual innovation. Our findings contribute to a better understanding of entrepreneurial team members’ responses to dynamic environment and their innovation behavior.
Relationship between Amount, Type, Enjoyment of Physical Activity and Physical Education Performance with Cyberbullying in Adolescents
Authors: Benítez-Sillero Juan de D. , Armada Crespo José M. , Ruiz Córdoba Esther , Raya-González Javier
Published: February 2021

(1) Background: Cyberbullying is a social concern in adolescents. The practice of physical activity is a key factor in protection against cyberbullying related to the multiple psychological benefits. Therefore, the study sets out to analyse the relationship between amount, types, enjoyment of physical activity and performance in physical education with cyberbullying. (2) Methods: A sample of 867 adolescents between 12 and 19 years of age from two schools in Andalusia (Spain) was studied. A computer-based questionnaire given in the classroom was used, with two questions about the type of physical activity, one on physical education performance, the Scale of Enjoyment of Physical Activity (PACES) and the Spanish version of the European Cyberbullying Intervention Project Questionnaire (ECIPQ). (3) Results: Clear relationships were found between the practice of physical activity and cybervictimisation. However, less involvement has been observed among girls who practice physical activity in cyberaggression. (4) Conclusions: In relation to the types of physical activity, it seems that practising physical activities that involve competition can help to develop defence mechanisms against cyberaggression, as well as improve values to be less involved in cyberaggression.
Feeling Gratitude and Depletion: The Ambivalent Consequences of Receiving Help in the Workplace
Authors: Zhan Yuanfang , Zhou Jinfan , Cheng Huan , Mu Renyan
Published: February 2021

Drawing from social exchange theory, we developed a dual-path model of employees’ reactions to episodic help received from colleagues. Through a diary study, using data collected from 127 full-time employees working in a large Chinese bank, we tested this model, revealing that receiving episodic help from colleagues is positively related to the help receivers’ gratitude and ego depletion. Through these two ambivalent psychological states, help receivers were found to simultaneously engage in more organizational citizenship behaviors and deviance behaviors on a daily basis. These empirical findings contribute to research that adopts a target-centric perspective in examining the consequences of helping behavior in the workplace.
The Motor Impact of the Static Balance in the Up Plank Position on Three Different Balls in Physical Activities of Physical Education Students
Authors: Badau Dana , Badau Adela , Manolache Gabriel , Ene Mircea Ion , Neofit Adriana , Grosu Vlad Teodor , Tudor Virgil , Sasu Radu , Moraru Raluca , Moraru Liviu
Published: February 2021

The present study aimed to assess the capacity of stability and motor impact in the development of the balance of student athletes by reducing the support surface on the ball in the up plank position, by using three categories of balls of equal size, but with different elasticity and weight. In this study, the second aim was to investigate the differences in maintaining static balance, on different balls, between those who are practicing individual sports or team sports. The total study sample include 48 students, active athletes (45.8% of individual sports and 54.2% of team sports), age X ± SD 18.74 ± 1.94 years. The research included two test sessions (initial and final) applied in two stages. The static balance tests were performed by measuring the time maintaining the up plank position with two and three points of support on the three balls, with different characteristics of elasticity and ranges of deformation: medical ball, handball ball and fitness ball. The results of the study showed that the superior initial and final results were recorded on the fitness ball, and the inferior results on the medicine ball. The upper difference was recorded at the up plank position with two support points (arms, legs) on the fitness ball, at 4980 s, and the lowest in the same test on the medical ball, at 3420 s. The largest difference was recorded at the up plank position with three support points on the handball ball, at 7.082 s, and the lowest in the same test on the medical ball, at 3.093 s. The subjects of the study perceived that the most difficult position to execute was the up plank position on the medical ball with two support points, with 43.8%, and the easiest stability was registered in the up plank position on the fitness ball with three points of support, of 37.5%. The relevance of the research results from the possibility of using different balls in conditions of positioning and body posture with a diminished support base in order to improve physical fitness.
Promoting Mental Health and Well-Being among Adolescent Young Carers in Europe: A Randomized Controlled Trial Protocol
Authors: Casu Giulia , Hlebec Valentina , Boccaletti Licia , Bolko Irena , Manattini Alessandra , Hanson Elizabeth
Published: February 2021

It is estimated that 4–8% of youth in Europe carry out substantial care for a family member or significant other. To prevent adverse psychosocial outcomes in young carers (YCs), primary prevention resilience building interventions have been recommended. We describe the study protocol of an international randomized controlled trial (RCT) of an innovative group intervention designed to promote the mental health and well-being of adolescent YCs (AYCs) aged 15–17. The RCT will be conducted in six European countries in the context of the Horizon 2020 European funded research and innovation project “Psychosocial support for promoting mental health and well-being among adolescent young caregivers in Europe” (“ME-WE”). The ME-WE intervention is based on Hayes and Ciarrochi’s psychoeducational model for adolescents and will consist of seven 2-h sessions in a group format, aimed to help AYCs build psychological flexibility and live according to their values. The control group will be a waitlist. Primary and secondary outcomes and control variables will be measured at baseline (T0), post-intervention (T1) and 3 months follow-up (T2). The COVID-19 pandemic has made amendments necessary to the original study protocol methodology, which we describe in detail. This study will contribute to building an evidence-based manualized program that educators and health and social care professionals can use to support AYCs in their transition to adulthood. From a research perspective, the outcomes of this study will contribute to evidence-based practices in primary prevention of psychosocial difficulties in AYCs and will gather novel knowledge on the effectiveness of Hayes and Ciarrochi’s model for use with middle adolescents with caring responsibilities. The trial has been preregistered (registration number: NCT04114864).
Enhancing Adolescent Girls’ Well-Being in the Arctic—Finding What Motivates Spending Time in Nature
Authors: Wiens Varpu , Soronen Kari , Kyngäs Helvi , Pölkki Tarja
Published: February 2021

Background: According to previous studies, the natural environment positively influences well-being, including that of adolescent girls. However, knowledge is lacking on what motivates adolescent girls to spend time in nature. A secondary analysis of qualitative data was conducted employing three preexisting sets of interview data that had formed the basis of previously published research reports. A novel perspective on what motivates adolescent girls in the Arctic to spend time in nature was uncovered—a finding that previous articles have not reported. Aim: The aim was to describe what motivates adolescent girls in the Arctic to spend time in nature. Methods: The participants were adolescent girls aged 13 to 16 living in the province of Finnish Lapland. The girls wrote about well-being (n = 117) and were interviewed (n = 19) about the meaning of seasonal changes, nature and animals’ influence on well-being. Also, five focus group interviews (n = 17) were held. The materials were analyzed by inductive content analysis. Results: After the secondary analysis, three generic categories were found: (1) wanting to have pleasant emotions, (2) the possibility of participating in activities and (3) a desire to feel better. The main category of “need to experience positive sensations” was formed. Conclusion: Based on these results, through personalized guidance and advice, it is possible to strengthen adolescent girls’ willingness to spend time in nature.
Why Do Muse Stem Cells Present an Enduring Stress Capacity? Hints from a Comparative Proteome Analysis
Authors: Acar Mustafa B. , Aprile Domenico , Ayaz-Guner Serife , Guner Huseyin , Tez Coskun , Di Bernardo Giovanni , Peluso Gianfranco , Ozcan Servet , Galderisi Umberto
Published: February 2021

Muse cells are adult stem cells that are present in the stroma of several organs and possess an enduring capacity to cope with endogenous and exogenous genotoxic stress. In cell therapy, the peculiar biological properties of Muse cells render them a possible natural alternative to mesenchymal stromal cells (MSCs) or to in vitro-generated pluripotent stem cells (iPSCs). Indeed, some studies have proved that Muse cells can survive in adverse microenvironments, such as those present in damaged/injured tissues. We performed an evaluation of Muse cells’ proteome under basic conditions and followed oxidative stress treatment in order to identify ontologies, pathways, and networks that can be related to their enduring stress capacity. We executed the same analysis on iPSCs and MSCs, as a comparison. The Muse cells are enriched in several ontologies and pathways, such as endosomal vacuolar trafficking related to stress response, ubiquitin and proteasome degradation, and reactive oxygen scavenging. In Muse cells, the protein–protein interacting network has two key nodes with a high connectivity degree and betweenness: NFKB and CRKL. The protein NFKB is an almost-ubiquitous transcription factor related to many biological processes and can also have a role in protecting cells from apoptosis during exposure to a variety of stressors. CRKL is an adaptor protein and constitutes an integral part of the stress-activated protein kinase (SAPK) pathway. The identified pathways and networks are all involved in the quality control of cell components and may explain the stress resistance of Muse cells.
Novel Mechanistic Insights and Potential Therapeutic Impact of TRPC6 in Neurovascular Coupling and Ischemic Stroke
Authors: Shekhar Shashank , Liu Yedan , Wang Shaoxun , Zhang Huawei , Fang Xing , Zhang Jin , Fan Letao , Zheng Baoying , Roman Richard J. , Wang Zhen , Fan Fan , Booz George W.
Published: February 2021

Ischemic stroke is one of the most disabling diseases and a leading cause of death globally. Despite advances in medical care, the global burden of stroke continues to grow, as no effective treatments to limit or reverse ischemic injury to the brain are available. However, recent preclinical findings have revealed the potential role of transient receptor potential cation 6 (TRPC6) channels as endogenous protectors of neuronal tissue. Activating TRPC6 in various cerebral ischemia models has been found to prevent neuronal death, whereas blocking TRPC6 enhances sensitivity to ischemia. Evidence has shown that Ca2+ influx through TRPC6 activates the cAMP (adenosine 3’,5’-cyclic monophosphate) response element-binding protein (CREB), an important transcription factor linked to neuronal survival. Additionally, TRPC6 activation may counter excitotoxic damage resulting from glutamate release by attenuating the activity of N-methyl-d-aspartate (NMDA) receptors of neurons by posttranslational means. Unresolved though, are the roles of TRPC6 channels in non-neuronal cells, such as astrocytes and endothelial cells. Moreover, TRPC6 channels may have detrimental effects on the blood–brain barrier, although their exact role in neurovascular coupling requires further investigation. This review discusses evidence-based cell-specific aspects of TRPC6 in the brain to assess the potential targets for ischemic stroke management.
Dysfunctional Gut Microbiome Networks in Childhood IgE-Mediated Food Allergy
Authors: Lee Khui Hung , Guo Jing , Song Yong , Ariff Amir , O’Sullivan Michael , Hales Belinda , Mullins Benjamin J. , Zhang Guicheng
Published: February 2021

The development of food allergy has been reported to be related with the changes in the gut microbiome, however the specific microbe associated with the pathogenesis of food allergy remains elusive. This study aimed to comprehensively characterize the gut microbiome and identify individual or group gut microbes relating to food-allergy using 16S rRNA gene sequencing with network analysis. Faecal samples were collected from children with IgE-mediated food allergies (n = 33) and without food allergy (n = 27). Gut microbiome was profiled by 16S rRNA gene sequencing. OTUs obtained from 16S rRNA gene sequencing were then used to construct a co-abundance network using Weighted Gene Co-expression Network Analysis (WGCNA) and mapped onto Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. We identified a co-abundance network module to be positively correlated with IgE-mediated food allergy and this module was characterized by a hub taxon, namely Ruminococcaceae UCG-002 (phylum Firmicutes). Functional pathway analysis of all the gut microbiome showed enrichment of methane metabolism and glycerolipid metabolism in the gut microbiome of food-allergic children and enrichment of ubiquinone and other terpenoid-quinone biosynthesis in the gut microbiome of non-food allergic children. We concluded that Ruminococcaceae UCG-002 may play determinant roles in gut microbial community structure and function leading to the development of IgE-mediated food allergy.
6,7,4′-Trihydroxyflavanone Protects against Dextran Sulfate Sodium-Induced Colitis by Regulating the Activity of T Cells and Colon Cells In Vivo
Authors: Lee Hyun-Su , Jeong Gil-Saeng
Published: February 2021

Colitis is a multifactorial disorder that mostly occurs in the gastrointestinal tract. Despite improvements in mucosal inflammation research, little is known regarding the small bioactive molecules that are beneficial for regulating T cells and colon cell activity. 6,7,4′-trihydroxyflavanone (THF) is a flavanone that possesses anti-osteoclastogenesis activity and exerts protective effects against methamphetamine-induced immunotoxicity. Whether THF mitigates intestinal inflammation by regulating T cells and colon cell activity remains unknown. In the present study, Jurkat and HT-29 cells were used for in vitro experiments, and dextran sulfate sodium (DSS)-induced colitis model in mice was used for in vivo experiment. We observed that THF did not have a negative effect on the viability of Jurkat and HT-29 cells. Quantitative PCR and Western blot analysis revealed that THF regulates the activity of Jurkat cells and HT-29 cells via the NFκB and MAPK pathways under stimulated conditions. In the DSS-induced colitis model, oral administration of THF attenuated the manifestations of DSS-induced colitis, including a reduction in body weight, shrinkage of the colon, and enhanced expression of pro-inflammatory cytokines in the colon and mesenteric lymph nodes. These data suggest that THF alleviates DSS-induced colitis by modulating the activity of T cells and colon cells in vivo.
Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis
Authors: Morishita Seita , Sato Takaki , Oosuka Shou , Horie Taeko , Kida Teruyo , Oku Hidehiro , Nakamura Kimitoshi , Takai Shinji , Jin Denan , Ikeda Tsunehiko
Published: February 2021

We previously reported that the bursa premacularis (BPM), a peculiar vitreous structure located above the macula, contains numerous cells expressing markers of lymphatic endothelial cells, such as podoplanin and LYVE-1. Herein, we examined the expression of lymphatic markers in the Berger’s space (BS), BPM, and vitreous core (VC). BS, BPM, and VC specimens were selectively collected in macular hole and epiretinal membrane patients during vitrectomy and were then immunostained with antibodies for podoplanin, LYVE-1, and fibrillin-1 and -2. By visualization using triamcinolone acetonide, the BS was recognized as a sac-like structure with a septum located behind the lens as well as BPM. Those tissues adhered to the lens or retina in a circular manner by means of a ligament-like structure. Immunostaining showed intense expression of podoplanin and LYVE-1 in the BS. Both BS and BPM stained strongly positive for fibrillin-1 and -2. The VC was faintly stained with antibodies for those lymph-node markers. Our findings indicate that both BS and BPM possibly belong to the lymphatic system, such as lymph nodes, draining excess fluid and waste products into lymphatic vessels in the dura mater of the optic nerve and the ciliary body, respectively, via intravitreal canals.
Formyl Peptide Receptor 2 Alleviates Hepatic Fibrosis in Liver Cirrhosis by Vascular Remodeling
Authors: Jun Ji Hye , Park Soo Young , Park Sohae , Park Hee Jung , Kim Jae Yeon , Park Gyu Tae , Bae Si Hyun , Kim Jae Ho , Kim Gi Jin
Published: February 2021

Hexapeptide WKYMVm (Trp-Lys-Tyr-Met-Val-D-Met), a ligand of formyl peptide receptor 2, exhibits anti-inflammatory and angiogenic properties in disease models. However, the therapeutic effects of WKYMVm on hepatic fibrosis have not been evaluated to date. Therefore, we investigated whether WKYMVm exerts antifibrotic effects and induces vascular regeneration in a rat model of bile duct ligation (BDL). The antifibrotic and angiogenic effects of WKYMVm on liver regeneration in the BDL rat model were analyzed using biochemical assays, qRT-PCR, western blotting, immunofluorescence, and immunohistochemistry. To determine the effects of WKYMVm on hepatic fibrosis and angiogenesis in vitro, we measured the expression levels of fibrotic factors in hepatic stellate cells (HSCs) and angiogenic factors in human umbilical vein endothelial cells (HUVECs). WKYMVm attenuated the expression of collagen type I (Col I) and α-smooth muscle actin (α-SMA) and significantly increased the levels of angiogenetic factors in the BDL model (p < 0.05). WKYMVm reduced fibrotic marker expression in transforming growth factor (TGF)-β-induced HSCs and promoted angiogenic activity through tube formation in 5-Fluorouracil (FU)-treated HUVECs (p < 0.05). Also, WKYMVm administration enhanced hepatocyte proliferation in BDL rats (p < 0.05). The WKYMVm alleviates hepatic fibrosis by inhibiting HSC activation and promotes hepatic regeneration via vascular remodeling. These data suggest that the WKYMVm may be a new therapeutic agent for liver fibrosis.
Deep Learning for Enterprise Systems Implementation Lifecycle Challenges: Research Directions
Authors: Hassanien Hossam El-Din , Elragal Ahmed
Published: February 2021

Transforming the state-of-the-art definition and anatomy of enterprise systems (ESs) seems to some academics and practitioners as an unavoidable destiny. Value depletion lead by early retirement and/or replacement of ESs solutions has been a constant throughout the past decade. That did drive an enormous amount of research that works on addressing the problems leading to the resource drain. The resource waste had persisted throughout the ESs implementation lifecycle phases and dimensions especially post-live phases; leading to depleting the value of the social and technical dimensions of the lifecycle. Parallel to this research stream, the momentum gained by deep learning (DL) algorithms and platforms has been exponentially growing to fuel the advancements toward artificial intelligence and automated augmentation. Correspondingly, this paper is set out to present five key research directions through which DL would take part as a contributor towards the transformation of the ESs state-of-the-art. The paper reviews the ESs implementation lifecycle challenges and the intersection with DL research conducted on ESs by analyzing and synthesizing key basket journals (list of the Association of Information Systems). The paper also presents results from several experiments showcasing the effectiveness of DL in adding a level of augmentation to ESs by analyzing a large set of data extracted from the Atlassian Jira Software Issue Tracking System across different ecosystems. The paper then concludes by presenting the research directions and discussing socio-technical research courses that work on key frontiers identified within this scholarly work.
Improvement in Quality of Life with Pelvic Floor Muscle Training and Biofeedback in Patients with Painful Bladder Syndrome/Interstitial Cystitis
Authors: Borrego-Jimenez Pedro-Santiago , Flores-Fraile Javier , Padilla-Fernández Bárbara-Yolanda , Valverde-Martinez Sebastián , Gómez-Prieto Agustín , Márquez-Sánchez Magaly Teresa , Mirón-Canelo José-Antonio , Lorenzo-Gómez María-Fernanda
Published: February 2021

Objective: To prove the benefits of pelvic floor muscle training with biofeedback (BFB) as a complementary treatment in women with bladder pain syndrome/interstitial cystitis (BPS/IC). Methods: Prospective, randomized study in 123 women with BPS/IC. Groups: BFB+ (n = 48): women with oral drug treatment (perphenazine and amitriptyline) plus intravesical instillations (sodium hyaluronate) plus pelvic floor muscle training with BFB; BFB−: (n = 75): women with oral drug treatment plus intravesical instillations. Variables: age, body mass index (BMI), time of follow-up, length of disease, time free of disease, diseases and health conditions concomitant, and responses to the SF-36 health-related quality of life questionnaire at the first consultation (SF-36 pre-treatment), and at the end of the study (SF-36 post-treatment). The treatment was considered successful when the SF-36 score reached values equal to or greater than 80 points or when the initial value increased by 30 or more points. Results: Mean age was 51.62 years old (23–82). BMI was higher in BFB−. The mean length of BPS/IC condition was 4.92 years (1–20), shorter in BFB+ than in BFB−. Mean SF-36 score pre-treatment was 45.92 points (40–58), lower in BFB+ than in BFB−. Post-treatment SF-36 score was higher than pre-treatment SF-36 score both in BFB+ and BFB−. SF-36 values were higher in BFB+ compared to BFB− over the follow-up. Conclusions: BFB improves quality of life in women with BPS/IC as adjunct therapy to combined oral and intravesical treatment.
Influence of COVID-19 on Health-Related Quality of Life and the Perception of Being Vaccinated to Prevent COVID-19: An Approach for Community Pharmacists from Romania and Bulgaria
Authors: Turcu-Stiolica Adina , Bogdan Maria , Subtirelu Mihaela-Simona , Meca Andreea-Daniela , Taerel Adriana-Elena , Iaru Irina , Kamusheva Maria , Petrova Guenka
Published: February 2021

Community pharmacists are essential front-line health workers, involved in relieving the COVID-19 burden. Their health-related quality of life status needs to be assessed, as lower levels could affect their functioning. In order to evaluate the current status of community pharmacists’ quality of life from Romania and Bulgaria during the COVID-19 pandemic, and to identify factors associated with their decision on being vaccinated to prevent COVID-19, an online survey involving 395 community pharmacists was conducted from 15th July 2020 to 15th August 2020. The 15D instrument was used for quality-of-life assessment. The pharmacists’ recommendations for vitamin C and D intake during the COVID-19 pandemic were also analyzed in order to promote future training programs for community pharmacists. Descriptive statistics, comparative analyses between pharmacists from Romania and Bulgaria, and multiple correlation analyses were performed on the collected data. Significant differences were observed for the level of quality of life between the two groups of pharmacists according to their age; smaller values, directly correlated with their age (total 15D score and age: Spearman r = 0.168, p = 0.022), were obtained for Bulgarian pharmacists regarding sleeping, usual activities, mental function, discomfort and symptoms, depression, distress. The perception of being vaccinated did not differ between Romanian and Bulgarian pharmacists, as almost 50% agreed to vaccination (p = 0.7542). Their willingness to vaccinate was correlated with vitamin D usage (p = 0.0134), rather than with vitamin C (p = 0.4157). No other significant associations were found between willingness to get vaccinated to prevent COVID-19 and other characteristics (age, gender, income, quality-of-life markers). Evidence-based interventions are required to enhance the health-related quality of life of community pharmacists involved in the first line of the COVID-19 pandemic.
Assessment of Disability Progression Independent of Relapse and Brain MRI Activity in Patients with Multiple Sclerosis in Poland
Authors: Kapica-Topczewska Katarzyna , Collin François , Tarasiuk Joanna , Czarnowska Agata , Chorąży Monika , Mirończuk Anna , Kochanowicz Jan , Kułakowska Alina
Published: February 2021

The aim of the study was to verify the association of clinical relapses and brain activity with disability progression in relapsing/remitting multiple sclerosis patients receiving disease-modifying treatments in Poland. Disability progression was defined as relapse-associated worsening (RAW), progression independent of relapse activity (PIRA), and progression independent of relapses and brain MRI Activity (PIRMA). Data from the Therapeutic Program Monitoring System were analyzed. Three panels of patients were identified: R0, no relapse during treatment, and R1 and R2 with the occurrence of relapse during the first and the second year of treatment, respectively. In the R0 panel, we detected 4.6% PIRA patients at 24 months (p < 0.001, 5.0% at 36 months, 5.6% at 48 months, 6.1% at 60 months). When restricting this panel to patients without brain MRI activity, we detected 3.0% PIRMA patients at 12 months, 4.5% at 24 months, and varying from 5.3% to 6.2% between 36 and 60 months of treatment, respectively. In the R1 panel, RAW was detected in 15.6% patients at 12 months and, in the absence of further relapses, 9.7% at 24 months and 6.8% at 36 months of treatment. The R2 group was associated with RAW significantly more frequently at 24 months compared to the R1 at 12 months (20.7%; p < 0.05), but without a statistical difference later on. In our work, we confirmed that disability progression was independent of relapses and brain MRI activity.
A Cognitive Behavioral Therapy-Informed Self-Management Program for Acute Respiratory Failure Survivors: A Feasibility Study
Authors: Hosey Megan M. , Wegener Stephen T. , Hinkle Caroline , Needham Dale M.
Published: February 2021

Background: The number of people surviving critical illness is rising rapidly around the globe. Survivorship comes at a cost, with approximately half of patients with acute respiratory failure (ARF) experiencing clinically significant symptoms of anxiety, and 32–40% of survivors having substantial anxiety symptoms in the months or years after hospitalization. Methods: This feasibility study reports on 11 consecutive ARF patients receiving up to six sessions of a psychological intervention for self-management of anxiety. Results: All 11 patients accepted and received the psychological intervention. Four patients did not fully complete all 6 sessions due to death (n = 1, 2 sessions completed), and early hospital discharge (n = 3, patients completed 2, 3 and 5 sessions). The median (IQR) score (range: 0–100; minimal clinically important difference: 13) for the Visual Analog Scale-Anxiety (VAS-A) pre-intervention was 70 (57, 75) points. During the intervention, all 11 patients had a decrease in VAS-A, with a median (IQR) decrease of 44 (19, 48) points. Conclusions: This self-management intervention appears acceptable and feasible to implement among ARF patients during and after an ICU stay.
Is Neoadjuvant Treatment Justified in Clinical T1 Pancreatic Ductal Adenocarcinoma?
Authors: Kim Hyung Sun , Nakagawa Kenji , Akahori Takahiro , Nakamura Kota , Takagi Tadataka , Sho Masayuki , Park Joon Seong , Yoon Dong Sup
Published: February 2021

Introduction: Studies on neoadjuvant treatment have been actively conducted in patients with resectable pancreatic cancer. However, neoadjuvant treatment effectiveness, especially in clinical T1 stage patients, still needs to be determined. We comparatively evaluated the oncologic benefit of preoperative neoadjuvant treatment in clinical T1 stage pancreatic cancer. Methods: Data from two centers were included in the comparative analysis, with overall and recurrence-free survival as primary outcomes, between January 2010 and December 2017. Results: In total, 45 patients were retrospectively reviewed in this study. Two patients in the neoadjuvant group were excluded because of distant metastasis during neoadjuvant treatment. Finally, 43 patients underwent a pancreatectomy for clinical T1 pancreatic cancer, of whom, 35 and 8 patients underwent upfront surgery and neoadjuvant treatment, respectively. Overall survival was similar in the two study groups (5-year overall survival rate: neoadjuvant group, 75%; upfront surgery group, 43.9%, p = 0.066). Conclusions: In our study on patients with clinical T1 stage pancreatic cancer, no significant differences were reported in the oncological outcome in the neoadjuvant therapy group. Large-scale prospective studies are needed to determine the survival benefits of neoadjuvant treatment for early-stage pancreatic cancer.
The Collagens DPY-17 and SQT-3 Direct Anterior–Posterior Migration of the Q Neuroblasts in C. elegans
Authors: Lang Angelica E. , Lundquist Erik A.
Published: February 2021

Cell adhesion molecules and their extracellular ligands control morphogenetic events such as directed cell migration. The migration of neuroblasts and neural crest cells establishes the structure of the central and peripheral nervous systems. In C. elegans, the bilateral Q neuroblasts and their descendants undergo long-range migrations with left/right asymmetry. QR and its descendants on the right migrate anteriorly, and QL and its descendants on the left migrate posteriorly, despite identical patterns of cell division, cell death, and neuronal generation. The initial direction of protrusion of the Q cells relies on the left/right asymmetric functions of the transmembrane receptors UNC-40/DCC and PTP-3/LAR in the Q cells. Here, we show that Q cell left/right asymmetry of migration is independent of the GPA-16/Gα pathway which regulates other left/right asymmetries, including nervous system L/R asymmetry. No extracellular cue has been identified that guides initial Q anterior versus posterior migrations. We show that collagens DPY-17 and SQT-3 control initial Q direction of protrusion. Genetic interactions with UNC-40/DCC and PTP-3/LAR suggest that DPY-17 and SQT-3 drive posterior migration and might act with both receptors or in a parallel pathway. Analysis of mutants in other collagens and extracellular matrix components indicated that general perturbation of collagens and the extracellular matrix (ECM) did not result in directional defects, and that the effect of DPY-17 and SQT-3 on Q direction is specific. DPY-17 and SQT-3 are components of the cuticle, but a role in the basement membrane cannot be excluded. Possibly, DPY-17 and SQT-3 are part of a pattern in the cuticle and/or basement membrane that is oriented to the anterior–posterior axis of the animal and that is deciphered by the Q cells in a left–right asymmetric fashion. Alternatively, DPY-17 and SQT-3 might be involved in the production or stabilization of a guidance cue that directs Q migrations. In any case, these results describe a novel role for the DPY-17 and SQT-3 collagens in directing posterior Q neuroblast migration.
Recent Advances in Integrative Multi-Omics Research in Breast and Ovarian Cancer
Authors: Khella Christen A. , Mehta Gaurav A. , Mehta Rushabh N. , Gatza Michael L.
Published: February 2021

The underlying molecular heterogeneity of cancer is responsible for the dynamic clinical landscape of this disease. The combination of genomic and proteomic alterations, including both inherited and acquired mutations, promotes tumor diversity and accounts for variable disease progression, therapeutic response, and clinical outcome. Recent advances in high-throughput proteogenomic profiling of tumor samples have resulted in the identification of novel oncogenic drivers, tumor suppressors, and signaling networks; biomarkers for the prediction of drug sensitivity and disease progression; and have contributed to the development of novel and more effective treatment strategies. In this review, we will focus on the impact of historical and recent advances in single platform and integrative proteogenomic studies in breast and ovarian cancer, which constitute two of the most lethal forms of cancer for women, and discuss the molecular similarities of these diseases, the impact of these findings on our understanding of tumor biology as well as the clinical applicability of these discoveries.
The 1β-Hydroxy-Deoxycholic Acid to Deoxycholic Acid Urinary Metabolic Ratio: Toward a Phenotyping of CYP3A Using an Endogenous Marker?
Authors: Magliocco Gaëlle , Desmeules Jules , Bosilkovska Marija , Thomas Aurélien , Daali Youssef
Published: February 2021

In this study, we assessed the potential use of the 1β-hydroxy-deoxycholic acid (1β-OH-DCA) to deoxycholic acid (DCA) urinary metabolic ratio (UMR) as a CYP3A metric in ten male healthy volunteers. Midazolam (MDZ) 1 mg was administered orally at three sessions: alone (control session), after pre-treatment with fluvoxamine 50 mg (12 h and 2 h prior to MDZ administration), and voriconazole 400 mg (2 h before MDZ administration) (inhibition session), and after a 7-day pre-treatment with the inducer rifampicin 600 mg (induction session). The 1β-OH-DCA/DCA UMR was measured at each session, and correlations with MDZ metrics were established. At baseline, the 1β-OH-DCA/DCA UMR correlated significantly with oral MDZ clearance (r = 0.652, p = 0.041) and Cmax (r = −0.652, p = 0.041). In addition, the modulation of CYP3A was reflected in the 1β-OH-DCA/DCA UMR after the intake of rifampicin (induction ratio = 11.4, p < 0.01). During the inhibition session, a non-significant 22% decrease in 1β-OH-DCA/DCA was observed (p = 0.275). This result could be explained by the short duration of CYP3A inhibitors intake fixed in our clinical trial. Additional studies, particularly involving CYP3A inhibition for a longer period and larger sample sizes, are needed to confirm the 1β-OH-DCA/DCA metric as a suitable CYP3A biomarker.
Effects of Both Fiber Post/Core Resin Construction System and Root Canal Sealer on the Material Interface in Deep Areas of Root Canal
Authors: Miura Hiroki , Yoshii Shinji , Fujimoto Masataka , Washio Ayako , Morotomi Takahiko , Ikeda Hiroshi , Kitamura Chiaki
Published: February 2021

This study aimed to examine the resin polymerization of a fiber post/core resin construction system and the interface between resin and root canal sealers, which are important for root canal sealing. We used the i-TFC Luminus fiber post and i-TFC Luminus LC flow (i-TFC-L), the GC fiber post and Unifil Core EM (GCF), and the FiberKor post and Build-It FR (FKP) as core construction systems, and Nishika Canal Sealer BG (CS-BG), Metaseal Soft (META), and Nishika Canal Sealer EN (CS-EN) as sealers. The light transmission of fiber posts (n = 5), the polymerization of core resin (n = 5), and the adhesion between the sealer and core resin (n = 10) were evaluated. The i-TFC Luminus fiber post light transmission was significantly higher than that of other posts. Without shielding, i-TFC-L showed a significantly greater amount of polymerized resin than the other systems. With shielding, although i-TFC-L showed a significantly greater amount of polymerized resin immediately after light irradiation, polymerized resin was significantly greater in GCF and FKP after 10 min. All systems adhered to CS-BG and META but not to CS-EN. These results indicate that resin polymerization in the cavity differs among fiber post/core resin construction systems and that the adhesion of the resin and sealer depends on the property of the sealer.