bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Formulation Strategy to Deliver Combined DNA and VLP Based HPV Vaccine
Authors: Uddin Mohammad Nasir , Henry Brittany , Carter Kewan D , Roni Monzurul a , Kouzi Samir S
Published: November 2019

Human papillomaviruses (HPV) are small, double-stranded DNA viruses that cause cervical cancer, the second most lethal cancer among women in the world. Currently, two vaccines are on the market for preventing HPV-caused cervical cancers and warts. Both are virus-like particle (VLP)-based vaccines. However, these vaccines have limitations; they are costly, have an invasive route of administration, require trained personnel to administer, need cold chain storage to preserve them, and most of all, they are preventive vaccines that do not have curative effects. Therefore, it is necessary to develop therapeutic HPV vaccines to facilitate the control of HPV-associated malignancies and to address all these issues. Recently there are DNA vaccines under investigation to prevent HPV. In general, DNA-based vaccines are better than or an excellent alternative to traditional vaccines since they can closely mimic live infections and can induce both antibody and cell-mediated immune responses. DNA vaccines involve the delivery of plasmid DNA (pDNA) which encodes the specific antigens. DNA vaccines have potential to be effective therapeutic tools against HPV infections. Combining the VLP-based and DNA-based vaccines can be highly effective as they can complement each other. VLP vaccines are more prone to mucosal immunity whereas DNA vaccines are more towards systemic immunity. In this article, we discuss an optimal formulation that will contain both type of vaccines, preventive and therapeutic. A film dosage form can be a good option which can be administered in buccal or sublingual routes for systemic action or in the vaginal area for local action to treat cervical cancer and to protect from future infection. Multiple vaccines in native form or in particulate form can be incorporated in film dosage forms. The film dosage form of vaccines can elicit both antibody-mediated (preventative) and cell-mediated (therapeutic) mechanisms. Film dosage forms are feasible to prepare for vaccine administration in the mouth cavity, GI tract, and vagina.
A Comparative Study of China and Japan’s Response to Section 301 Investigations of the United States
Authors: Guifen Pei , Xinying Wang ,   Institute of Japanese Research, Hebei University, Hebei, Baoding, China.,   Institute of Japanese Research, Hebei University, Hebei, Baoding, China.
Published: November 2019

Japan is the country with the most Section 301 investigations initiated by the United States. Meanwhile, the ongoing Section 301 investigation case against China is the most complicated and tough case until now. The different responses of Japan and China will be the core theme of this paper. Originally, Japan, little by little, accepted all the demands of the United States under American pressure in the semiconductor conflict and then began to resist its unreasonable demands; eventually, Japan forced the United States to withdraw its excessive requirement in auto parts conflict. In the case of China, previous Section 301 investigations were resolved by bilateral or multilateral agreements although it was difficult. In this time, the Chinese government has taken a countermeasure against the United States’ bullying, evident from the very beginning. The situation is that China and the United States conducted bilateral negotiations on the stretch. Meanwhile, the United States continued to extend the scope of tariff goods and escalate the tariff rate against China, and the Chinese government immediately published the same amount and tariff rate for imported products from the United States. China and other countries are jointly suffering the sanction from the United States and are trying to restrain the trade hegemony of the United States.
US–China Trade War: Russia’s Interests
Authors: Ignatova Olga V. , Gorbunova Olga A. , Tereshina Olga Yu. ,   Department of World Economy and World Finance,   Department of World Economy and World Finance,   Department of Language Training; Financial University under the Government of the Russian Federation, Moscow, Russia
Published: November 2019

The article is devoted to one of the urgent problems of the world economy: the trade opposition of the United States and China. Due to the fact that these countries occur to be the largest economies in the world, their conflict cannot in one way or another be reflected in other subjects of international economic relations. The article analyzes the main stages of the trade war between the United States and China and formulates the causes of the crisis. On the basis of a regional approach and analysis of statistical data, it became possible to make an assessment of the effects that the US–PRC rivalry has on mutual trade, investment, and energy cooperation between Russia and China. It is noted that in connection with the trade conflict, Russian–Chinese relations are reaching a new level of development, and the number of joint economic projects is growing. However, the confrontation between the United States and China brings not only opportunities but also risks for Russia. The authors make a forecast about the impact of the trade war on the economy of the Russian Federation in the short and medium term.
US–China Trade War: The Tale of Clash Between Biggest Developed and Developing Economies of the World
Authors: Kalsie Anjala , Arora Ashima ,   Department of Management Studies, University of Delhi, New Delhi 110007, India.,   Shaheed Sukhdev College of Business Studies, University of Delhi, New Delhi 110089, India.
Published: November 2019

United States (US) and China are the two largest economies of the world, where the former is the biggest developed economy and the latter is one of the largest developing economies of the world. This paper implores to assess the cause and effect of the US-Sino Trade War. The attempt is to understand the reasons behind the same and its impact on warring economies and collateral damage to the economies of other countries. The experience establishes that trade wars have no winners. The war started in year 2018; the long-run effects of this trade war are still to be seen yet; till date the impact of this crisis has been substantial for both the US and China.
An Interrelationship between Blood Pressure, Body Roundness Index, Waist Circumference, and Waist to Height Ratio
Authors: Jeppu Ashok K. , Kumar Kavitha A. ,   Department of Biochemistry,   Department of Otorhinolaryngology; International Medical School, Management and Science University, Shah Alam, Malaysia.
Published: November 2019

Obesity is a global epidemic. Obesity related comorbidities include hypertension, diabetes, dyslipidemia, obstructive sleep apnea, and sudden death. Abdominal adiposity has been blamed for causing cardiovascular complications. The body roundness index (BRI) has been considered to be a superior anthropometric measure for abdominal adiposity. The aim of the study was to find the relationship of blood pressure in healthy individuals to BRI, waist circumference (WC), and waist to height Ratio (WHtR). It also tried to find out the relation of BRI to WC and WHtR. This cross-sectional study was conducted on 300 individuals aged between 18 and 60 years who were apparently healthy (not on any treatment or physical training) and willing to participate in this study. A structured questionnaire was used to collect the data. Parameters like height, weight, and WC were measured. BRI and WHtR were calculated. The data was analyzed using descriptive and inferential statistics. We observed that BRI was well correlated to the WC, body mass index (BMI), and WHtR. Both the systolic and diastolic blood pressure correlated well with BRI. BRI and WHtR, both are good parameters to evaluate the blood pressure of an individual.
Effect of Incubation with Dimethyl Sulfoxide on the Mitotic Cycle of Cell Culture of Rabbit Dermal Papilla
Authors: Oksana Novikova , Galyna Bozhok , Tatyana Bondarenko ,   Department of Cryoendocrinology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences, Kharkov, Ukraine.,   Department of Cryoendocrinology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences, Kharkov, Ukraine.,   Department of Cryoendocrinology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences, Kharkov, Ukraine.
Published: November 2019

At cryopreservation, the issue of preserving the integrity and cytogenetic stability of dermal papilla (DP) cells of the hair follicle is topical. The study examined the effect of various concentrations of the cryoprotectant dimethyl sulfoxide (DMSO) (5-15%), as well as its combination with 5% bovine serum albumin (BSA), on the mitotic index (MI) and the number of pathological mitoses in the cell culture of DP on the first and second passages. It has been established that the treatment of cells with DMSO in concentrations of 10% and above leads to a significant increase in the number of pathological mitosis. This is accompanied by the decrease in MI (when processing concentrations of DMSO below 10%) at the first passage or a complete cessation of proliferation at the second passage (when processing concentrations of DMSO above 10%). The combination of 5% DMSO with BSA is optimal for minimizing toxic effects on the genetic apparatus of DP cells and can be used as part of cryopreservation media.
Effect of Cryopreservation on Different Passages of Porcine Dorsal Root Ganglion Cell Culture
Authors: Ali Sabina , Bozhok Galina ,   Department of Cryoendocrinology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine.,   Department of Cryoendocrinology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine.
Published: November 2019

The cell culture obtained from dorsal root ganglia (DRG) is a valuable model used in biology or medical research. However, the effect of cryopreservation on the properties of DRG-derived cell culture of different passages remains unclear up to date. The objective of the study is to assess the effect of cryopreservation with various concentrations of cryoprotectant dimethyl sulfoxide (DMSO) on the viability and morphological features of porcine neonatal DRG cell culture of different passages. Cell suspension was obtained from DRG of neonatal piglets and cultured in an α-MEM nutritional medium supplemented with 10% fetal calf serum (0, 1st, and 2nd passages). Cells of different passages were cryopreserved at a cooling rate of 0.5°C/min to – 20°C (step 1) and 1°C/min to – 80°C (step 2) followed by immersion into liquid nitrogen. Cryoprotective solutions based on α-MEM nutrient medium and 25% fetal calf serum (FCS) containing 5%, 7.5%, and 10% DMSO were used. It was established that the primary culture (passage 0) consisted of four cell morphological types: large rounded cell bodies of sensory neurons (SN) and three types of non-neuronal cells, namely, polygonal cells with pronounced elongated processes (type 1), spindle-shaped cells (type 2), and multipolar flattened fibroblastoid cells (type 3). As the number of passages increases, an elimination of SN from the culture, a decrease in the relative number of 1st and 2nd cell types, and expansion of 3rd cell type were observed. DRG cell culture had sufficiently high resistance to cryopreservation as cell viability was in the range from 83% to 90% using different concentrations of DMSO. The cells of passage 1 were more resistant to cryopreservation in comparison with primary culture cells (passage 0). The best result was achieved by freezing the culture of passage 1 in the cryoprotective medium with 7.5% DMSO, where 90.6% of viable cells were observed after thawing.
Adjunct Faculty Perceptions of Participation in Online Collaborative Research Teams
Authors: Hartman Rita , Kearns-Sixsmith Danielle , Akojie Patricia , Banton Christa
Published: November 2019

Career professionals who serve as adjunct faculty at the university level are expected to engage in continual research and publishing to maintain their status as adjunct (part-time) faculty, to be considered for potential advancement, and to qualify for additional compensation.  One way of meeting this objective is to participate in online collaborative research projects benefiting from a set of multiple lenses, multiple insights, and a multitude of considerations in regard to design, methodology, data interpretations, and broader reaching implications.  A narrative inquiry approach was applied to gain an in-depth understanding of the experiences of adjunct faculty working in online collaborative research teams. Data was gathered through phone interviews where adjunct faculty shared their personal experiences and reflections about working as collaborative researchers in an online environment. Using an inductive process, themes were drawn from the responses of the participants to address the research question. The dominant themes found were organizational skills, interpersonal skills, and personal growth and development. The results of the study led to recommendations for supporting adjunct faculty in online collaborative research for building a sense of scholarly community and expanding opportunities for personal professional growth.
A Rare Case of Granulomatous Pneumonitis Due to Intravesical BCG for Bladder Cancer
Authors: Clérigo Vera , Castro Ana , Mourato Teresa , Gomes Conceição
Published: November 2019

Granulomatous pneumonitis is a rare complication of bacillus Calmette-Guerin immunotherapy following intravesical administration of bacillus Calmette-Guerin. The authors present an unusual case of a 67-year-old man who developed mild and non-specific symptoms, following intravesical bacillus Calmette-Guerin instillations. Examinations revealed features of miliary tuberculosis and granuloma suggestive of mycobacterial infection. Anti-tuberculosis treatment resulted in a remarkable improvement in his symptoms and gradually upgrading of radiological appearance. The symptoms were less severe than some others described but this case provides evidence that, even in some cases, specific treatment may be necessary. We highlight the importance of recognizing miliary Mycobacterium bovis as a probable complication of bacillus Calmette-Guerin immunotherapy. The clinical disease course can be mild, despite extensive bilateral miliary nodules on primary presentation.
Diagnostic Delay of Multiple Sclerosis in a Portuguese Population
Authors: Aires Ana , Barros Ariana , Machado Célia , Fitas Diogo , Cação Gonçalo , Pedrosa Rui , Cerqueira João , Perdigão Sandra , Silva Ana Martins da , Vale José , Sá Maria José , Andrade Carlos
Published: November 2019

Introduction: Multiple sclerosis is a chronic inflammatory disease, in which a diagnostic delay could reduce the available therapeutic options. Therefore, it is important to monitor the time to diagnosis and understand factors that may potentially reduce it. The objective of this study was to determine the time between the first symptoms and the diagnosis of multiple sclerosis and which factors may contribute to a diagnostic delay.Material and Methods: Cross-sectional multicenter study, with retrospective data analysis, conducted in five tertiary Portuguese hospitals. Patients were consecutively selected from each local multiple sclerosis patients´ database. Sociodemographic and initial clinical data were collected through a questionnaire. Date of final diagnosis and multiple sclerosis classification was obtained from clinical files.Results: A total of 285 patients were included with mean age at diagnosis of 36 years. The median time between first clinical manifestation and multiple sclerosis diagnosis was nine months (IQR 2 - 38). Diagnostic delay was associated with an older age (p < 0.001; r = 0.35), motor deficit at onset [26.5 months (IQR 4.5 - 56.5); p = 0.0005], higher number of relapses before diagnosis (p < 0.001; r = 0,626), first observation by other medical specialty [11 months (IQR 2 - 48); p < 0.001], prior alternative diagnosis [20 months (IQR 4 - 67.5); p < 0.001] and primary progressive subtype [37 months (IQR 25 - 64.5); p < 0.001]. The most significant delay occurred between the initial symptom and neurological observation.Discussion: A significant delay occurred between initial symptoms and the diagnosis of multiple sclerosis, reflecting the need toincrease awareness of this entity and its diverse symptom presentation.
Lodging Resistance as Related to Root Traits for Mechanized Wet Seeding of Two Super Rice Cultivars
Authors: Zhang Minghua , Mo Zhaowen , Liao Juan , Pan Shenggang , Chen Xiongfei , Zheng Le , Luo Xiwen , Wang Zaiman
Published: November 2019

The mechanical hill wet-seeded rice machine is benefits to establish uniform seedling, and ditches were established by using this machine. However, little knowledge is known on the effect of the establishment of ditches on growth, lodging and yield, and their relationship with root traits. In this study, two field experiments were conducted during 2012 and 2013 with using two super rice varieties (i.e. hybrid rice ‘Peizataifeng’ and inbred rice ‘Yuxiangyouzhan’) grown under three ditches establishment treatments (i.e. T1: both water ditches and seed ditches were established by the machine, T2: seed ditches were established by the machine, T3: neither water nor seed ditches were established by the machine). The lodging index and lodging resistance traits, the grain yield and above-ground dry weight and the root traits were measured. The results showed that the lodging index was significantly affected by the treatments with ditches. The strongest lodging resistance was detected in mechanical hill wet-seeded rice with ditches treatment in both 2012 and 2013. The lodging resistance was strongly related to the breaking resistance, the root volume and root superficial area at the heading stage and maturity stage and the total root length at the heading stage. No significant difference was investigated in grain yield or dry weight of mechanical hill wet-seeded rice. Yuxiangyouzhan showed higher grain yield, dry weight and better lodging resistance but unfavorable root growth attributes than Peizataifeng. Therefore, the mechanical hill wet-seeded rice with ditches treatment increased rice lodging resistance is related to root traits.
A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications
Authors: Cacchio Paola , Del Gallo Maddalena
Published: November 2019

Bacterial Calcium-carbonate Precipitation (BCP) has been studied for multiple applications such as remediation, consolidation and cementation. Isolation and screening of strong calcifying bacteria is the main task of BCP-technique. In this paper we studied CaCO3 precipitation by different bacteria isolated from a rhizospheric soil in both solid and liquid media. It has been found, through culture-depending studies, that bacteria belonging to Actinobacteria, Gammaproteobacteria and Alphaproteobacteria are the dominant bacteria involved in CaCO3 precipitation in this environment. Pure and mixed cultures of selected strains were applied for sand biocementation experiments. Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analyses of the biotreated samples revealed the biological nature of the cementation and the effectiveness of the biodeposition treatment by mixed cultures. X-ray diffraction (XRD) analysis confirmed that all the calcifying strains selected for sand biocementation precipitated CaCO3, mostly in the form of calcite. In this study Biolog® Eco-plate is evaluated as a useful method for a more targeted choice of the sampling site with the purpose of obtaining interesting candidates for BCP applications. Furthermore, ImageJ software was investigated, for the first time to our knowledge, as a potential method to screen high CaCO3 producer strains.
Comparative Health-Related Fatty Acid Profiles, Atherogenicity and Desaturase Indices of Marula Seed Cake Products from South Africa and Eswatini
Authors: Mthiyane Doctor Mziwenkosi Nhlanhla , Hugo Arno
Published: November 2019

Marula seed cake (MSC) is a nutritionally-rich natural feed resource that can enhance the healthiness of animal-derived foods (ADFs) for human consumption. This study compared the health-related fatty acid (FA) profiles of MSC products from South Africa and Eswatini. Composite samples monthly collected from both countries were analysed for FAs. MSC products from both countries were found to be dominated by oleic acid (>70%), followed by palmitic, linoleic and stearic acids. Consequently, both products had their FA totals dominated by ƩMUFA followed by ƩSFA, ƩPUFA, Ʃn-6 PUFA and Ʃn-3 PUFA. Both oleic and stearic acids were higher (P < 0.01) whilst linoleic (P < 0.001), α-linolenic (P < 0.05), margaric (P < 0.05), palmitoleic (P < 0.05) and eicosatrienoic (P < 0.05) acids were lower in South African in comparison to Eswatini MSC. Consequently, South African MSC had higher ƩMUFA (P < 0.01) but lower ƩPUFA (P < 0.001), Ʃn-6 PUFA (P < 0.001) and Ʃn-3 PUFA (P < 0.05). Also, Eswatini MSC had higher n-6 : n-3 PUFA, PUFA : SFA (P = 0.001) and PUFA : MUFA (P < 0.05) ratios. Further, MSC products from both countries had similarly (P > 0.05) low atherogenicity and high desaturase indices. In conclusion, both country products are rich particularly in oleic acid and their incorporation into farm animal diets would increase content of the MUFA in ADFs and, consequently, improve health benefits to human consumers.
Use of a Taguchi Model in Hibiscus sabdariffa Extracts Encapsulated by Spray-Drying
Authors: Navidad-Murrieta Migdalia Sarahy , Pérez-Larios Alejandro , Sánchez-Burgos Jorge Alberto , Ragazzo-Sánchez Juan Arturo , Luna-Bárcenas Gabriel , Sáyago-Ayerdi Sonia G.
Published: November 2019

Aqueous and ethanolic extracts of Hibiscus sabdariffa were spray-drying using maltodextrin (MD) and gum arabic (GA) as carriers agents. An experimental design Taguchi L8 with seven variables was implemented. Physicochemical properties in the encapsulates were evaluated by UV-Vis, XRD, spectroscopy and gravimetric techniques. Treatments with aqueous extracts showed the highest concentration of total soluble polyphenols (TSP) 32.12- 21.23 mg EAG/g DW, and antioxidant capacity (AOX) for ABTS assay. The best treatment for TSP and AOX was T4: 2.5% Hibiscus w/w, aqueous extracts, decoction, extract-to-carrier ratio 1:1 (w/w), proportion to carriers (MD:GA) 80:20 (w/w), 10000 rpm, 150°C. Taguchi L8 model is a tool that allows the use of multiple variables with a low number of treatments that indicate the drying conditions that give the best parameters, focusing mainly on TSP and AOX, in addition, is a good alternative for the preservation and stability of the PC in Hibiscus.
Statistical Models in Comparative Optinalysis through Induced Systematic Skewization Mechanisms
Authors: Abdullahi Kabir Bindawa
Published: November 2019

The effect of sensitivity points (sequence order and position of every element) of sequences following comparative optinalysis under two proposed mechanisms, the paranodic and synodic skewization, was studied to develop a modeling approach to comparative optinalysis of sequences. The results show that the outcomes of comparative optinalysis (similarity measurement) in a set of paranodically skewed sequences can be modeled deterministically by suitable line regression functions. The sensitivity points (nodes) of a sequence display two important and distinct zones, the K-zones and the B-zones. Continues paranodic skewization within these zones (KB zones) operates within probability space, but at hyperskewization level and at the K-zones only, the outcomes of comparative optinalysis operate outside the probability space. Moreover, the outcomes of comparative optinalysis by synodic skewization can be modeled deterministically by some regression line functions, but a general regression function cannot be identified. At certain limit of skewization value space, following paranodic and synodic skewization, the outcomes of comparative optinalysis at the left-sided sequence form a similar pattern with the right-sided sequence.
Selective Dorsal Rhizotomy for the Treatment of Gait Dysfunction in Cerebral Palsy
Authors: Chen Brian Po-Jung , Wang Kemble K. , Novacheck Tom F.
Published: November 2019

» Objective physical examination measures should be developed and improved to identify and measure spasticity and to differentiate it from other types of movement abnormalities.

» Using gait analysis as part of the preoperative evaluation of selective dorsal rhizotomy (SDR) can improve the safety and efficacy of this treatment of gait dysfunction in cerebral palsy.

» A multidisciplinary team is crucial when assessing and managing children with cerebral palsy because spasticity is only one disease component of cerebral palsy and differentiating between different types of hypertonia is challenging.

» A modest percentage of rootlet division (25% to 40%) reliably eliminates spasticity in cerebral palsy.

» Long-term studies have shown that SDR is safe and may improve certain aspects of function.
The Affect Of The Asean China Free Trade Agreement (Acfta) On The SME's Performance
Authors: Setiyono Wisnu Panggah , Andriyani Dewi , Putra Boy Isma
Published: November 2019

This research is aimed to analyze the influence of ASEAN-China Free Trade Agreement (ACFTA) on the financial performance of SMEs in Sidoarjo. The financial performance of SMEs in this study was measured by the level of sales comparation between the period before and after ACFTA. This research applied mix method technique with squids mixed method strategy, especially sequential explanatory strategy. It is a strategy for the researchers to combine the data that was found from one method with the other method. The steps were begun with the researcher collecting the quantitative data by performing different test or Paired samples T-test then continued with collecting the qualitative data by doing library research. The results show that  there are significant differences in financial performance; first, it is seen from the sales between before and after ACFTA, and second,  it is proven that  that the existence of ACFTA significantly affects the sales rate of SMEs.
Determinants and Consequences of Extended Disclosure under Concentrated Ownership Environment: The Indonesian Evidence
Authors: Ghofar Abdul
Published: November 2019

This study is aimed at providing evidence in regard with (1) the economic consequences of quality of extended disclosure and (2) the influence of ownership and director char- acteristics on the quality of extended disclosure. Using 260 firm years of the Indonesian firms, this study finds that the increase of disclosure quality is found to have a positive correlation with the increase of share price five (5) days after the announcement date of financial reports and the increase of Return of Assets (ROA). In regard with ownership and director characteristics, this study finds that government ownership and size of board have a positive relationship with disclosure quality; while management and block-holder ownership are not relevant in increasing disclosure quality. It is also found that individual characteristics of director which are independence and financial expertise/background do not have any relationship with the quality of extended disclosure. From the study, it can be concluded that extended disclosure is an effective tool to increase the financial performances of a firm under a concentrated ownership environment such as in Indone- sia. Government and board directors are two players who induce firms to increase trans- parency through voluntarily disclosure.
Pengembangan Model Pembelajaran Blended Learning (Syncronous vs Asyncronous) Pendidikan Jasmani Kesehatan Dan Rekreasi
Authors: Mardikaningsih Agusti , Kurniasari Praharisti
Published: November 2019

One of the success  learning process is appropriat learning design and in accordance with the conditions of learners and the learning environment. The purpose of designing a study is to improve and enhance the quality of learning. Based on the observations made by the researcher team teaching theoretical subjects in students found especially PJKR tendency kinesthetic learning pattern is a pattern of learning absorb information through physical practice. The purpose of this research is the development of a draft development know Learning Blended Learning Model (syncronous vs. Asynchronous) at the Teachers' Training College Budi Utomo. To learn more about whether blended learning (syncronous and asyncronous) appropriate for use in student sports that have these problems, it is essential that research efforts further to the researchers took the title of Model Development Learning Blended Learning (syncronous vs. Asynchronous) On Student Education Department of Sport Teachers' Training College Budi Utomo Malang. This development research using ADDIE Model. ADDIE Model is a model of the learning system of a general nature and is one of the models in the product development, application of the ADDIE model of instructional design facilitates the learning environment tailored to the existing problems. Each stage in the model ADDIE evaluations are available, so that each stage will affect the next stage. Based on trials that have been carried out to subject matter experts, media specialists and students can be concluded that the Development Model of Learning Blended Learning In Teachers' Training College Student Department of Sport Education Budi Utomo Malang have valid criteria / feasible. It is necessary for sustainability and cooperation in implementing the model.
Deskripsi Keterkaitan antara Pembelajaran IPA terhadap Aspek Sopan Santun
Authors: Cahyaningsih Dwi , Marpaung Eva Suhaeni
Published: November 2019

The purpose of this study was to describe the relationship between the ability of students in science subjects on aspects manners in SMP Negeri 3 Jambi. The method used in this research is descriptive method, where research is conducted to find out the value of variables independently, either one or more variables without making comparisons, or connecting with other variables. In this study, there were 50 respondents consisting of 25 students of class VII A and 25 students of class VII B. The instrument used was a questionnaire. The results showed that students in the class VII A has politeness in the high category, while students in grade VIII B has a politeness in the low category.
Model Pembelajaran Inkuiri Terbimbing dan Pengaruhya terhadap Keterampilan Proses Sains Siswa
Authors: Adirahayu Mega Fauziyah , Wulandari Fitria Eka
Published: November 2019

The purpose of this study is to find out whether there is an influence of the learning model Guided inquiry on the learning outcomes of science process skills of students at SMP Negeri 2 Wonoayu. The design of this study is the non-equivalent control group design. The samples used in this study were 2 classes with sampling techniques. Where from the two classes, one class is used as the experimental class, which is given treatment by using the guided inquiry learning model, while the next class is used as the control class by using the learning model that is usually used in the class. The research instrument used was a matter of science process skills with data collection techniques using the test method in the form of pre-test and post-test questions. Data analysis techniques are carried out using the "t" test. From the "t" test, the results show that there is a significant influence between the guided inquiry learning model on science process skills.
SATISFACTION WITH USE OF PUBLIC HEALTH AND PEER LED FACILITIES FOR HIV PREVENTION SERVICES BY KEY POPULATIONS IN NIGERIA
Authors: Ochonye Bartholomew , Folayan Morenike Oluwatoyin , Fatusi Adesegun O , Emmanuel Godwin , Adepoju Tomi , Ajidagba Babatunde , Jaiyebo Toluwanimi , Umoh Paul , Yusuf Ayo
Published: November 2019

Background: The aim of the study was to identify the proportion of female sex workers, men who have sex with men, and people who inject drugs who had accessed HIV prevention services at public health facilities and peer-led facilities, their level of satisfaction with these services, and perceived barriers and challenges to accessing HIV services from public and peer-led HIV prevention service providers. Methods: A mixed-method approach was used to collect data from key populations in the four states in Nigeria. Quantitative data collected included level of satisfaction with and barriers to use of public and peer-led facilities. In-depth interviews and focus-group discussions were conducted to explore reasons for satisfaction with and barriers to use of services. Descriptive and bivariate analyses were was conducted for quantitative data. Qualitative data were summaried, emerging themes identified, described and quotes reflecting the themes corresponding to interview questions highlighted Results : Nine hundred sixty-seven persons responded to questions on the use of public health or/and peer-led facilities. Two hundred thirty-eight (49.4%) respondents had received HIV and sexual and reproductive health services through public health facilities, and 236 (48.7%) had received the services through peer-led facilities. Significantly more respondents were satisfied with the quality of services provided by peer-led organisations than with public health facilities with respect to service providers listening to respondent’s problems and concerns (p=0.007),privacy and confidentiality (p=0.04) and respect of rights of service recipients (p=0.04). Significantly more respondents using peer-led organisations than those using public health facilities identified no barriers to service access (p=0.003). More respondents using public health facilities than peer-led facilities identified cost of services (p=0.01), confidentiality (p=0.002), waiting time (p<0.01) and staff attitude (p=0.001) as barriers to service access. Thee was no difference in the proportion of respondents willing to discontinue their use of either facilities (p=0.08). Qualitative data revealed that concerns with access of services at the public health facility were due mainly to stigma and the effects of the same-sex prohibition law. Conclusion: Key populations were more satisfied receiving HIV prevention services at peer-led organisations than at public health facilities.
Annual Alveolar Bone Loss in Older Adults Taking Oral Bisphosphonate: A Retrospective Cohort Study
Authors: Helmi Mohammad , AlOsaimy Sara , Goodson J. Max , Hasturk Hatice , Natto Zuhair S.
Published: November 2019

Background: Although several studies assessed the effect of bisphosphonate (BIS) administration on alveolar bone loss, this relationship has not been fully investigated using longitudinal analysis. The aim of the this article is to predict annual alveolar bone loss in a subpopulation of older adults patients who were taking oral bisphosphonate (BIS), adjusting for systemic diseases and associated risk factors. Methods: This is a retrospective cohort study. We identified all subjects who reported receiving oral bisphosphonate from 2008–2015 (N=30) using the electronic health records of each patient to identify suitable radiographs for analysis. For the longitudinal data analysis, 26 subjects were eligible for inclusion, having at least two exposures of the complete mouth set or repeated bitewing radiographs at least a one-year interval; they were then matched on age and sex to another 26 patients who did not report receiving bisphosphonate at any point of their life. Results: Mild periodontitis was higher in the BIS group compared to the no BIS group; however, moderate periodontitis was higher in the no BIS group. For those who did not take oral BIS, change over time was not significant after the two-year period. However, the BIS group had experienced 0.088 mm more bone loss compared to the no BIS group (95% CI: 0.001, 0.176. P-value = 0.048), adjusting for all other variables included in the model. Conclusion: The group that reported receiving oral bisphosphonates showed no improvement in maintaining alveolar bone level, and the use of oral BIS may not be effective in reducing annual alveolar bone loss; however, emerging evidence is promising for the use of bisphosphonate as an adjunctive local delivery medication for the management of periodontal diseases.
Development of a high number, high coverage dog rabies vaccination programme in Sri Lanka.
Authors: Soriano Carlos Sánchez , Gibson Andrew D. , Gamble Luke , Bailey Jordana L. Burdon , Green Samantha , Green Mark , Bronsvoort Barend M. deC. , Handel Ian G. , Mellanby Richard J. , Mazeri Stella
Published: November 2019

Background: Rabies is estimated to cause 59,000 deaths and economic losses of 8.6 billion USD every year. Despite several years of rabies surveillance and awareness programmes, increased availability of post-exposure prophylaxis vaccinations and dog population control, the disease still remains prevalent in Sri Lanka. This study reports the development and roll-out of a high number, high coverage canine rabies vaccination campaign in Sri Lanka, providing estimates for the vaccination coverage achieved, analysing the local dog demographics, and identifying barriers of attendance to static vaccination clinics. Methods: A mass dog vaccination campaign was undertaken in Negombo, Sri Lanka. The campaign was composed of static point and door-to-door vaccination stages, with a final survey of vaccination coverage. A large volume of data on the distribution, health, and signalment of vaccinated dogs was collected through a mobile phone application. A logistic regression model was developed to investigate which socio-spatial and dog-related factors influenced attendance of owners to static vaccination points. Results: The campaign vaccinated over 7,800 dogs achieving a vaccination coverage of 75.8%. A dog:human ratio of 1:17 was estimated. Most dogs were owned, and the dog population was mostly male, adult, and non-sterilized. Unawareness, unavailability and handling problems were the most common reasons given by owners to explain failure to attend a static vaccination point. The regression analysis showed that increasing distance to a static point, in addition to young age and poor health of the dog, were associated with a decrease in the likelihood of attendance to a static vaccination points. Conclusion: This study demonstrates the feasibility of high number, high coverage vaccination campaigns in Sri Lanka. The information on dog ecology and barriers of attendance to a static point vaccination clinic will facilitate development of future vaccination campaigns.
Efficacy and safety of two Ayurvedic dosage forms for Allergic rhinitis: Study protocol for an open-label randomized controlled trial
Authors: Dahanayake JM , Perera Pathirage Kamal , Galappaththy P , Samaranayake D
Published: November 2019

Background: Allergic rhinitis (AR) is an immune response of the nasal mucosa to airborne allergens and involves nasal congestion, watery nasal discharge, itching of the nose and sneezing. The symptoms of allergic rhinitis may significantly affect a patient’s quality of life and can be associated with conditions such as fatigue, headache, cognitive impairment and sleep disturbances. Various complementary and alternative medicine treatments have been used for this condition in clinical practice. The Ayurveda system of medicine is the most common complementary medicine system practiced in Sri Lanka. The aim of this study is to examine the efficacy and safety of a decoction used in traditional Ayurveda for allergic rhinitis and it’s ready to use freeze dried formulation in comparison to an antihistamine over a period of 4 weeks on relief of symptoms in allergic rhinitis. Study design: This is a three arm, open label, non-inferiority, randomized controlled clinical trial enrolling patients with AR. Tamalakyadi decoction containing 12 ingredients (TMD12), used in traditional Ayurveda and its freeze dried formulation are the test products. The efficacy and safety of the two Ayurvedic dosage forms will be tested against the antihistamine loratidine Patients with symptoms of AR will be allocated randomly into the 3 arms after a 1 week run-in period and the medications will be given orally for 28 days. Total Nasal symptom Score (TNSS) of the patients will be used as the primary efficacy endpoint. TNSS will be recorded and compared between the 3 arms prior to visit 1, at the end of 28 days, end of the and second months of follow up. Symptom scores of daytime nasal symptoms, night time nasal symptoms, non-nasal symptoms and Health Related Quality of Life questionnaire are used as secondary end points. Discussion: This clinical trial will be able to provide evidence based scientific data on Ayurvedic dosage form, TMD12 and the freeze-dried formulation in the treatment of allergic rhinitis. This trial is expected to develop capacity to scientifically evaluate various Ayurvedic treatments that are claimed to have efficacy in treatment of various disease conditions. Trial registration:ISRCTN18149439 (06 May 2019) Keywords: Allergic rhinitis, Ayurvedic dosage forms, Tamalakyadi decoctions, Randomized controlled trial
Optimised protocol of QIAamp® DNA mini Kit for bacteria genomic DNA extraction from both pure and mixture sample
Authors: Yang Zhong
Published: November 2019

As the sequencing technology developed, Next Generation Sequencing (NGS) has been used by more and more microbiology lab from different fields. Meanwhile , the quality of DNA will highly influence the sequencing result. Recently, there is an increasingly requirement for effective DNA extraction protocol to get high quality DNA from both pure and mixture cultures. Especially for researchers who what to study the microorganisms from complex mixture samples from food and environment. In this protocol, we modified the protocol of a commercial DNA extraction kit from Qiagen, and made it suitable for DNA extraction of different sample sources. The optimized protocol is proved to be effective. The DNA extracted with this protocol has been sent for NGS and get many interesting result.
Development of HealthRESPECT; An integrated service model for older long-term care hospital/nursing home patients using ICT
Authors: Choi Jung-Yeon , Kim Kwang-il , Lim Jae-Young , Ko Jin Young , Yoo Sooyoung , Kim Hongsoo , Lee Minho , Jang Sae-Kyun , Lee Dong Hee , Lee Jungwoo , Jung Young-il , Oh In-Hwan
Published: November 2019

Background: In Korea, fast aging country, an optimal healthcare service model for older adults should be developed. The purpose of this study is to identify the current healthcare system and to develop new healthcare service model using information and communication technologies (ICT) for older patients. Methods: Qualitative literature review, focus group interview and structured survey were conducted to identify current healthcare systems for older patients. Afterwards, we developed healthcare service model which include contests of comprehensive geriatric assessment, disease management, drug management, rehabilitation and consultation service using ICT. Result: Sixty (23 hypertension, 18 diabetes and 19 heart failure) articles are reviewed. ICT based chronic disease management models was effective and patients’ satisfaction were improved. In focus group interview and structured survey, current patients and medical information transfer between hospitals was inconvenient, costly and time consuming, especially in long-term care hospital (LTCH) and nursing home (NH) and the necessity of service model to manage older patients using ICT was confirmed. ICT based service model for older patients dwelling LTCH and NH was developed (Health-RESPECT) which include evidence based 1) comprehensive geriatric assessment (CGA) and establishment of customized management strategy 2) chronic disease management service with decision support system 3) potentially inappropriate medication management service 4) rehabilitation service and 5) consultation and videoconference service. Conclusion : This study is meaningful to identify current healthcare systems in older adults and to develop a system to manage older patient living in LTCH and NH using ICT. However, Health-RESPECT service model will need to be validated in further study.
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets
Authors: López-Serrano Sergi , Galofré-Milà Nuria , Costa-Hurtado Mar , Pérez-de-Rozas Ana M. , Aragon Virginia
Published: November 2019

Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3-4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to the serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, selected strains were tested in phagocytosis assays and again variability was observed in the susceptibility to alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.
Parents' and caregivers' perceptions of the use of live video recording in neonatal units: A focus group study.
Authors: BRIS Aude LE , MAZILLE Nadia , SIMONOT Pauline , LUHERNE Maude , FLAMANT Cyril , GASCOIN Geraldine , Laighin Gearóid Ó , HARTE Richard , PLADYS Patrick
Published: November 2019

To study the perceptions of parents and caregivers on the use of live video in neonatal clinical practice in order to better assess its suitability, limits and constraints.Methods: Nine focus group interviews were conducted in four neonatal units involving 20 caregivers and 19 parents. Data were triangulated using transcripts and field notes and analyzed using inductive and semantic thematic analysis.Results: The seven major themes that emerged from the caregiver focus groups were (i) the impact of video recording on caregiver’s behavior; (ii) the impact on parents; (iii) forensic issues ;(iv) guarantee of use; (v) benefits for the new-born; (vi) methodology of use; and (vii) technical considerations & feasibility. The five major themes emerge from parents focus groups were i) benefits for the new-born and care enhancement; (ii) impact on parents and potential benefits in case of newborn child/parent separation; (iii) informed consent and guarantee of use;(iv) concern about a possible disruptive impact on caregivers; and (v) data protection.Conclusion: Both parents and caregivers found the use of video in care useful and acceptable if measures were taken to protect the data and mitigate any negative impacts on caregivers.