bluuebunny/crossref_metadata_2025 huggingface dataset:
Antibiotic resistance’s Genotypic and Phenotypic Characteristics and the Frequency of Virulence Factors in P. aeruginosa Isolates Isolated from Water Samples in Iran
Authors: Ghorbani Ghasem , Rahimi Ebrahim , Shakerian Amir
Published: September 2022

Pseudomonas aeruginosa is a pathogenic bacterium that can contaminate water. In this study, 430 water samples were evaluated for P. aeruginosa, antibiotic resistance, and the abundance of virulence factors. P. aeruginosa was isolated from 28 (6.51%) water samples. Among the types of water, well and spring water showed the highest P. aeruginosa with, respectively, 20 (15.6%) and 5 (8.06%) positive samples per type of samples. Drinking water and mineral water showed minor contamination with P. aeruginosa. The prevalence of antibiotic resistance against meropenem, imipenem, erythromycin, gentamicin, chloramphenicol, and enrofloxacin was zero. The lowest and highest prevalence of antibiotic resistance was observed in drinking water and well water, respectively. The most abundant genes encoding antibiotic resistance in the P. aeruginosa were blaTEM, blaCTX-M, and blaSHV. This study also showed that the most abundant virulence genes in the Pseudomonas aeruginosa strain isolated from water were algD (15 = 3.49%), lasB (11 = 2.56%), toxA (10 = 2.32%), and exoS (7 = 1.63%). This study suggests that water may be a source of P. aeruginosa and contribute to releasing resistance genes through the food chain. Cross‐contamination is the water transfer process that can cause contamination with P. aeruginosa in water. Therefore, hygienic principles can be effective in reducing water contamination.
Simulation Analysis of 4G/5G OFDM Systems by Optimal Wavelets with BPSK Modulator
Authors: Domathoti Bullarao , Ch Chengaiah , Madala SrinivasaRao , Berhanu Afework Aemro , Rao Yamarthi Narasimha
Published: September 2022

LTE (Long-Term Evolution) is one of the appropriate technologies to sever the requirement of the IoT (Internet of Things) together with mobile UE (User Equipment), because of priority services, high data rate, low latency, etc. LTE has received OFDM for the transmission because it meets the necessity of LTE-like range flexibility and empowers the cost gainful responses for the wide carriers. LTE offers big-data values, allows spectrum reframing, reduces cost, etc. We proposed a 4G/5G adopted OFDM based on the multi-equalizer and BPSK (Binary-Phase Shift Keying). The BER (Bit Error Rate) of the system is reduced through the optimal wavelet coefficient at the transmitter side. This optimal wavelet coefficient is obtained by applying a DWT (Discrete Wavelet Transform) to the input signal through a BFF (Binary Firefly) algorithm. The serial to parallel process is carried out through the BPSK modulation. At last, the multi-equalizer like MMSE (Minimized Mean Square Error) and MLE (Maximum Likelihood) is applied to the OFDM model to reduce the BER. The system is analyzed using the parameter like SER (Symbol Error Rate), spectral efficiency, MSE (Mean Square Error), and BER. The proposed methodology provides a better outcome than the other existing approaches.
The Preventive Effects of Naringin and Naringenin against Paclitaxel-Induced Nephrotoxicity and Cardiotoxicity in Male Wistar Rats
Authors: Khaled Shimaa S. , Soliman Hanan A. , Abdel-Gabbar Mohammed , Ahmed Noha A. , Attia Kandil Abdel Hai Ali , Mahran Hesham A. , El-Nahass El-Shaymaa , Ahmed Osama M.
Published: September 2022

This study assessed the preventive properties of naringin and naringenin on paclitaxel-induced nephrotoxicity and cardiotoxicity in adult male Wistar rats. Intraperitoneal injection of paclitaxel 2 mg/kg body weight, two days/week on the 2nd and 5th days of each week, with or without oral administration of naringin and/or naringenin 10 mg/kg body weight every other day, was continued for six weeks. Treatment of rats with naringin and/or naringenin significantly reversed elevated serum creatinine, urea, and uric acid levels caused by paclitaxel, reflecting improved kidney function. Similarly, heart dysfunction induced by paclitaxel was alleviated after treatment with naringin and/or naringenin, as evidenced by significant decreases in elevated CK-MB and LDH activities. After drug administration, histopathological findings and lesion scores in the kidneys and heart were markedly decreased by naringin and/or naringenin. Moreover, the treatments reversed renal and cardiac lipid peroxidation and the negative impacts on antioxidant defenses via raising GSH, SOD, and GPx. The preventive effects of naringin and naringenin were associated with suppressing oxidative stress and reestablishing antioxidant defenses. A combination of naringin and naringenin was the most efficacious in rescuing organ function and structure.
Serologic Presentation of Lamotrigine-Induced Lupus
Authors: Dempsey Hannah , Aitcheson Gabriella , Goble Gretchen , Snook Riley
Published: September 2022

This paper discusses the presentation of a rare drug side effect, a case of drug-induced lupus presenting with weight loss, weakness, hepatitis, and pancreatitis. A 24-year-old male with a history of major depressive disorder and childhood seizures presented to the ER with symptoms of abdominal pain, significant weight loss, and weakness. Initial workup revealed acute pancreatitis, elevated liver function enzymes (LFTs), and abnormal anti-double-stranded DNA antibody (anti-dsDNA) 1 : 640. He showed no classical clinical signs of lupus including rash, arthritis, or photosensitivity. He had multiple hospitalizations in the previous 6 months for excessive weight loss, malnutrition, weakness, and altered mental status. He had been taking lamotrigine for seizure prevention and mood stabilization while on a selective serotonin reuptake inhibitor (SSRI) and had a decline in health since the lamotrigine dose was increased. Antihistone antibodies were positive suggesting a drug-induced lupus syndrome. We hope to bring awareness to the possible rare complication of lamotrigine-induced lupus.
Development of Network Security Based on the Neural Network PSD Algorithm
Authors: Li Jianxun , Ji Song , Jiang Yiran
Published: September 2022

The more frequent occurrence of network security incidents has an impact on network security. Through the research on network security situational awareness, this paper constructs a multilevel network security situation evaluation index system from various aspects and uses the Elman neural network optimized by the genetic algorithm to evaluate network security situation. Aiming at the disadvantage of subjective dependence in the traditional assignment method of basic probability assignment function, Elman neural network is used to obtain the basic probability assignment function to increase its objectivity, and it is optimized with the PSD algorithm. In addition, the neural network is further improved by the genetic algorithm. In the traditional D-S evidence theory, an evidence correction step is added to optimize the situation that the final judgment result is incorrect due to evidence conflict. Finally, the fusion rules of the D-S evidence theory are used to fuse the support degrees of the four first-level situations to different security levels to obtain the final network security situation assessment result. The results show that the prediction accuracy of the GA-Elman neural network model is as high as 80%, which is significantly higher than that of the traditional D-S model, indicating that the model proposed in this paper has improved the accuracy of the assessment and prediction results. In conclusion, this study provides feasible theoretical prediction guidance for the accurate assessment of network security posture, reveals the improvement ideas for network security development, and is of great significance for the maintenance of network environment security.
The Development of Indirect Carotid Cavernous Fistulas after Microvascular Ischemic 4th Nerve Palsies
Authors: Pickel Lauren , Micieli Jonathan A.
Published: September 2022

An indirect carotid cavernous fistula (CCF) is an abnormal connection between the cavernous sinus and internal or external carotid artery. Indirect CCFs often occur spontaneously, particularly in the setting of vascular risk factors such as hypertension, diabetes, and atherosclerosis. Microvascular ischemic nerve palsies (NPs) share these vascular risk factors. However, to date, no temporal relationship between microvascular ischemic NP and indirect CCF occurring sequentially has been reported. We describe the cases of 64- and 73-year-old women who developed indirect CCFs within 1–2 weeks after spontaneous resolution of a microvascular ischemic 4th NP. Both patients had complete resolution and an asymptomatic period between the 4th NP and CCF. This case highlights the shared pathophysiology and risk factors between microvascular ischemic NPs and CCFs, and emphasizes that CCFs should be kept in the differential diagnosis for red eye or recurrent diplopia in patients with previous microvascular ischemic NP.
Posterior Scleritis in a Patient with Psoriasis Masquerading as Acute Angle Closure Glaucoma
Authors: Doumazos Spyridon , Kandarakis Stylianos A. , Petrou Petros , Karagiannis Dimitrios , Doumazos Leonidas , Georgalas Ilias
Published: September 2022

Posterior scleritis is a relatively uncommon form of scleral inflammation of the posterior segment of the eye. Clinical manifestations include ocular pain, headache, pain with ocular movements, and loss of vision. A rare presentation of the disease is acute angle closure crisis (AACC) with elevated intraocular pressure (IOP) secondary to anterior displacement of the ciliary body. Various systemic diseases have been described to co-exist with posterior scleritis; however, psoriasis has not been associated with posterior scleritis. Here, we present a case of posterior scleritis which initially presented as AACC in a patient with pre-existing psoriasis. A 50-year-old male with a history of psoriasis under treatment presented to the emergency department with intense sudden ocular pain and loss of vision on the left eye as well as headache and nausea. A thorough medical and ocular history was taken, and a detailed examination of the anterior and posterior segment was completed including visual acuity and IOP. Initial diagnosis of AACC was made, and appropriate actions were taken with partial resolution of his symptoms. However, upon further work-up including ultrasound (B-scan) of the left eye a final diagnosis of posterior scleritis was made. The patient was treated with steroids and nonsteroidal anti-inflammatory drugs which dramatically improved his condition. Photographic evidence of initial presentation and post-treatment condition was obtained and presented in this report. Posterior scleritis is a potentially vision-threatening condition which is usually challenging to diagnose. In this report, we highlight the challenges one might come into when dealing with different manifestations of the same disease, increasing awareness. This case of posterior scleritis presenting as AACC in a patient with a history of psoriasis enhances what we already know from the literature as well as provides some new insights in the clinical manifestations of posterior scleritis in the setting of psoriasis without arthritis.
Recent developments in offshore wind energy systems: Technologies and practices
Authors: Vasudevan Saravanan , Kondayampalayam Venkatachalam Moorthy , Murugesan Arumugam
Published: September 2022

This paper deals with offshore wind power generation technologies, power transmission and grid communication features, and associated power system studies for effective implementation of offshore wind energy systems, explaining its various stages of implementation. Also, this paper reviews the latest trends in offshore wind energy systems, addressing various aspects like large wind farm siting, power evacuation studies, cable selection, high-voltage direct current/flexible alternating current transmission systems (HVDC/FACTS) technology options, reliability evaluation, and autonomous monitoring. India's renewable power generation capacity through off-shore wind generation is also outlined to ensure low carbon energy emissions with improved energy efficiency. The policy and regulatory framework factors for reaching five gigawatts (GW) of offshore wind projects in the states of Tamilnadu and Gujarat by the year 2032 using current methods and advanced technology are discussed here. This goal can be accomplished using current practices and advanced technologies. For effective implementation of offshore wind farms, suitable measures and likely actions by various stakeholders are suggested.
Critical success factors for smart-professional disruptor in university
Authors: Pornpongtechavanich Phisit , Eumbunnapong Kawitsara , Daengsi Therdpong , Nilsook Prachyanun
Published: September 2022

Current and emerging technologies have changed a lot. Consequently, every year, Gartner Technology has made many new changes in accordance with global developments. For example, in terms of artificial intelligence (AI), mixed reality (MR), extended reality (XR), collaboration platforms, online learning, distributed cloud, internet of behaviors (IoB), and cybersecurity. Due to changes in technology, disruptors have to constantly learn new technology in order to be up to date in the transfer of knowledge to learners. Therefore, in this research, critical success factors (CSFs) have been studied, which help them become highly skilled professionals by developing their own skills with technology to be a successful disruptor at university. The study found the CSFs, which were derived from the synthesis of international research papers. Disruptors' success consists of 12 internal and 10 external success factors. Smart-professional disruptors in universities were assessed using a focus group method with eight experts. Focus group results found that there were seven important internal factors for smart-professional disruptors in universities and seven minor internal factors. Including all internal factors, smart-professional disruptors have 14 factors; external factors are the most important ones for smart-professional disruptors in universities. In total, smart-professional disruptors have a total of 11 external factors.
Analyzing the science achievement test: Perspective of classical test theory and Rasch analysis
Authors: Haw Law Hui , Sharif Sabariah Bte , K. Han Crispina Gregory
Published: September 2022

Assessment in teaching and learning allows both instructor and students to evaluate the achievement, performance and improve upon it. The achievement test appears as one of the most commonly used assessment tools to determine students' gains in the cognitive domain. The study analysed science achievement test (SAT), a multiple-choice test for grade 10 senior secondary school students. The 50 SAT items were developed in compliance with the Malaysian science curriculum specification. The face and content validity of SAT were validated by experts in the science field. The SAT was administered with 50 students in a pilot study. The collected data was analysed using item analysis based on classical test theory (CTT) and Rasch analysis. In terms of difficulty index (p), item analysis showed that there were five difficult items, 33 moderately difficult items, and 12 easy items. Findings also reported eight items presented as poor items due to the poor discriminating power. The SAT showed a good coefficient of 0.862 for Kuder-Richardson 20 (KR-20) and 0.851 for the split half method. While the Rasch analysis showed a reliable and good separation for both item and person. Besides, the Rasch analysis displayed that there are two items should be refreshed or removed. The study revealed that the SAT was a solid, valid, and reliable tool, which is suitable to be used to measure the student’s achievement in science.
Academic dishonesty on online learning among vocational high school students
Authors: Jatmika Surya , Suwandi Joko , Santoso Jarot Tri Bowo , Oktaviana Faizah Laila , Karima Mutiara
Published: September 2022

This study described the academic dishonesty phenomenon in accounting subject online learning at the business and management vocational high schools and identify the factors that influenced students to commit academic dishonesty. This was case study research with a qualitative approach. The participants were four teachers and six students from accounting and institutional finance classes. The data were collected using interviews and documentation, then being analyzed using interactive analysis techniques. The results showed that most students’ academic dishonesty behaviors were cheating and collaborating during examinations or assignments and doing individual assignments together. Several factors related to academic dishonesty among students include: i) Rationalization, stating rational reasons about the teacher not explaining the material thoroughly, which impacted students’ lack of material understanding; ii) Pressure, which comes from a desire within the students and parents’ demands to get good grades; iii) Opportunity, which arises due to the lack of direct teacher supervision on students’ work and the openness of internet access; and iv) Lack of academic ability, showing that the students who commit academic dishonesty are usually lacking in terms of academic ability.
The effect of demographic factors on environmental knowledge among university students
Authors: Sukri Akhmad , Rizka Muhammad Arief , Sakti Hadi Gunawan , Lukitasari Marheny , Purwanti Elly
Published: September 2022

This study explored and revealed the demographic factors that influence students' environmental knowledge (EK) in Indonesia. This survey research involved 1,345 students from 15 universities across Indonesia. The research employed a validated questionnaire on EK and was modified to match the circumstances in Indonesia. The instrument consisted of six statements with five scales namely 1=strongly disagree, 2=disagree, 3=indifferent, 4=agree, and 5=strongly agree. Five demographic variables were tested together to determine their effect on students' EK. Descriptive analysis, analysis of variance (ANOVA), and multiple linear regression were used to test the independent variables. The result of the study revealed that gender, study program, grade point average (GPA), and parents’ occupation variable have an effect simultaneously on students' EK (p<.05) with a contribution of 6.2% (R square=.062), while the students’ grade variable has no effect on EK (p>.05). Female students have better EK than male students (p<.05). The trend showed that the higher the GPA, the higher the EK of the student (p<.05). Hence, the trend showed that students whose parents have lower income tend to have better EK than those whose parents have higher income. Students in science education and computer course have better EK than science, non-science education, and social humanities study programs. This study implicated the importance of introducing environmental insights through education to increase students' EK in higher education.
Evaluation of the learning management system and its relationship in the perception of engineering students
Authors: Flores-Cáceres Richard , Dávila-Ignacio Carlos , Ortega-Galicio Orlando , Morales-Romero Guillermo , Trinidad-Loli Nicéforo , Caycho-Salas Beatriz , Auqui-Ramos Elizabeth , León-Velarde César , Auqui-Ramos Renan
Published: September 2022

The objective of this study was to identify the results of the learning management system (LMS) functionality, and its relationship in the perception of electronic engineering students. The results serve as a basis for continuous improvement for the higher institution, because these systems are tools that have the purpose of improving the performance and retention of the student in the virtual teaching-learning process. Initially, the reliability of the data collected was determined using Cronbach's Alpha, obtaining a consistency coefficient of 0.868. After processing the data in the SPSS software, using the 5-level Likert scale, it was determined that the indicators that present a better perception are related to the design and ease of navigation. However, 21.4% do not fully agree with the functionality, due to the technical problems presented when downloading the study material. The effect generated by the optimal functionality of the LMS is 70.87% satisfaction in students. The Chi square test validated the cause-effect relationship, with a significance of 0.000, between the functionality of the LMS, on the perception of the students. It determined that the indicators with a greater relationship refer to design, availability (connectivity) and ease of communication and interaction with the teacher and their peers.
The scientific argumentative skill analysis reviewed from the science literacy aspect of pre-service teacher
Authors: Fakhriyah Fina , Rusilowati Ani , Nugroho Sunyoto Eko , Saptono Sigit , Ridlo Saiful , Mindyarto Budinaini , Susilaningsih Endang
Published: September 2022

Scientific argumentative skills could encourage the critical thinking skill of college students about scientific phenomena. This research aimed to measure and describe the scientific argumentative skill of the primary school teacher candidates, reviewed from the scientific literacy. This research applied the quantitative method by using a survey typed cross-sectional design. The researchers selected the participants with convenience sampling. The results were 184 college students of even semester of 2020/2021. The applied instrument was the validity process. The data collecting techniques were test and questionnaire assisted with a Google Form. The test instrument consisted of four items. Then, the questionnaire item of the student’s perceptions about the argumentative scientific skills consisted of ten items. The researchers arranged the instruments with the indicators of science literacy-based scientific argumentative skills about biodiversity. The validity results showed that the test and questionnaire instruments were valid, relevant, and reliable to measure the skills. The researchers measured the argumentative scientific skills with Toulmin argumentative pattern (TAP) that was modified. The scientific argumentative skill profiles of the students showed that a percentage of 52% students was at level 3. However, there were no students reaching level 5. The students’ perceptions showed that they were interested in expressing their arguments and confidence while expressing their opinions. The results suggested the science educators prepare teacher candidates with argumentative scientific skills by developing learning innovation. The empowerment strategy by using scientific argumentation measurement is essential to apply to create better primary school teacher candidate with 4.0 century skills.
Effects of integrating a brain-based teaching approach with GeoGebra on problem-solving abilities
Authors: Mohd Yatim Siti Seri Kartini , Saleh Salmiza , Zulnaidi Hutkemri , Yatim Siti Ainor Mohd
Published: September 2022

B-Geo Module is a module that has been developed using the brain-based teaching approach (BBTA) integrated with GeoGebra Software (B-Geo Module) is expected to help students’ problem-solving abilities of the topic of Differentiation. The BBTA is a strategy that uses brain-based learning techniques. It was created to be consistent with the individual brain's tendencies and ideal functions in order to ensure that pupils can learn efficiently. Therefore, the proposed of this study is to explore the possible effects of the B-Geo Module on problem-solving abilities for the Topic of Differentiation. A quasi-design of pre-test and post-test experiments was utilized in this study, which included 118 form 4 pupils from rural secondary schools. For school selection, the researchers employed cluster sampling approaches, and for sample selection, they used an intact group. The schools were separated into two groups: experimental and control. The experimental group used the B-Geo Module, while the control group used traditional ICT modules. The instrument used was the Problem-Solving Test of Differentiation. The results of the data analysis showed the effectiveness of the B-Geo Module in the problem-solving abilities in the topic of Differentiation among rural secondary school pupils. The multimedia such as GeoGebra can be the tools for BBTA to facilitate Additional Mathematics teachers in secondary schools to help students solve problem and improved their learning in the topic of Differentiation.
Towards improving soft skills of medical education in the 21st century: A literature review
Authors: Riskiyana Rilani , Qomariyah Nurul , Hidayah Rachmadya Nur , Claramita Mora
Published: September 2022

Global challenge forced medical education to be transformative in preparing the future health care professional. Student-centered learning (SCL) has been regarded as the most suitable medical curriculum approach to meet the health care demand. Indonesia, with its hierarchical and collectivistic culture, has long implemented SCL. The necessary skills are needed to be identified to improve the medical curriculum in Indonesia. We did a literature review to generate soft skills for the health care professionals. We distributed the list to stakeholders and medical teachers for a two-round Delphi study. We identify 26 soft skills through literature review. The Delphi study's first-round yielded 95 responses comparing the expectation and reality of the 26 soft skills in the current medical curriculum. The second round Delphi study was performed towards the similar participants to gather the final set of soft skills recommended for curriculum improvement in medical and health professions education. Soft skills, though considered necessary, are not well-understood yet. The unfamiliarity influenced the irresolute delivery of soft skills in medical schools. Faculty developments are recommended to improve the understanding and capability of teachers to facilitate the learning of soft skills.
Organizational commitment building to the principal of the senior high school
Authors: Budiarti Ratna , Sujanto Bedjo , Mukhtar Mukhneri
Published: September 2022

This quantitative study aimed to determine the effect of personality and decision-making on the commitment of principals from high schools in the state of DKI Jakarta, Indonesia. The survey method was used in this study, along with path analysis for testing hypotheses. This was done with 89 random selection principals as respondents. This study revealed that personality has a direct positive influence on organizational commitment. There is a positive direct decision-making influence on organizational commitment. Hence, there is also an indirect influence on organizational commitment through decision-making. This paper can be a reference to improving the quality of the school by having a principal who has a strong commitment to his school, supported by a brave personality to get out of his comfort zone and is ready to sacrifice for the school’s progress.
Promoting higher-order thinking skills during online learning: The integration of metacognition in science for higher education
Authors: Maryani Ika , Prasetyo Zuhdan Kun , Wilujeng Insih , Purwanti Siwi
Published: September 2022

This study aimed to explore the integration of metacognition in online science education for college students and tested the feasibility of the learning model on students’ high order thinking skills (HOTS). The analyze, design, develop, implement, and evaluate (ADDIE) model was employed in this study. A needs analysis was conducted through interviews and questionnaire surveys to 21 science lecturers from primary school teacher education study programs at seven state universities and 14 private universities in Indonesia. In the development phase, the effectiveness of the model was examined through an experimental study involving three groups of students: experimental group (41 students), control group 1 (39 students), and control group 2 (39 students). The experimental study was performed using the randomized pretest-posttest comparison group design. The research hypothesis was investigated using a general linear model and multivariate analysis of variance. Through awareness-building, essential questioning, planning, monitoring, evaluating, and reflecting, this study successfully integrated metacognition into online science education. The model's learning syntax incorporated both synchronous and asynchronous learning activities. Virtual and contextual projects are critical components of this approach because they demonstrate how metacognition is regulated. Expert judgment indicated that the model under development was highly feasible. The experimental study established that the learning model had a considerable effect on students’ HOTS, which rose by 75% (a large effect) due to the model’s implementation.
Desenvolvimento de roteiros turísticos no município de Santana do Livramento, RS, Brasil: um estudo sobre a Ferradura dos Vinhedos
Authors: Bielinski Michele , Cassanego Junior Paulo Vanderlei
Published: September 2022

A construção de roteiros com elementos típicos de um território atrai turistas e, por isso, torna-se uma alternativa às regiões que não possuem atrativos consolidados. Nesta perspectiva, este estudo visa analisar o processo de construção, desenvolvimento e participação das organizações locais no roteiro turístico Ferradura dos Vinhedos, em Santana do Livramento, no Rio Grande do Sul, mediante as etapas de roteirização do Ministério do Turismo do Brasil. Por meio de abordagem qualitativa e método de estudo de caso, foram realizadas 21 entrevistas, com a técnica bola de neve. A análise de conteúdo demonstrou que há fases do processo de roteirização que não foram realizadas parcial ou totalmente; e que o envolvimento de agentes públicos, privados e sociais é escasso, influenciando na promoção, comercialização e legalização do roteiro turístico. Por fim, o roteiro apresenta oportunidade de atrair novos turistas e investidores para o município, todavia, faz-se necessária a revisão do processo de roteirização e o estímulo dos agentes locais.
Quem conta um conto aumenta um ponto? Uma revisão do uso do storytelling por empresas e destinos turísticos
Authors: Barbosa José William de Queiroz , Souza Anderson Gomes de
Published: September 2022

A contação de histórias faz parte do processo de construção de significados coletivos e da identidade dos indivíduos. Em Por que estudar narrativas, Motta afirma que é a partir delas que os sujeitos se expressam, se apresentam e produzem significados no mundo. O storytelling tem como premissa a construção de narrativas para a criação de vínculos afetivos entre indivíduos, histórias e personagens, aumentando a capacidade deles em atribuir sentido e assimilar mensagens. Segundo Mossberg, em Storytelling and destination development, no turismo, o storytelling é usado como estratégia para maximizar a experiência do turista. O objetivo desta investigação é analisar os diferentes usos da técnica do storytelling pelos empreendimentos e destinos turísticos. Trata-se de um estudo de caráter qualitativo, cujos dados foram obtidos por meio de pesquisa documental e bibliográfica, segundo a análise categorial de conteúdo proposta por Bardin. Os resultados mostraram que o storytelling é cada vez mais adotado pelas empresas e pelos destinos turísticos que buscam se destacar no mercado. Os dados indicaram que a aplicação do storytelling foi positiva, sobretudo na atração de clientes, tanto no âmbito privado quanto no público.
Argostemma tortilobum (Rubiaceae), a new species from Vietnam
Authors: FANG KE-YI , TONG YI-HUA , VU TIEN-CHIN , NGUYEN KHANG-SINH , XIA NIAN-HE
Published: September 2022

Argostemma tortilobum, a new species endemic to Vietnam, is described and illustrated. The species is morphologically most similar to A. sarmentosum, but differs in having erect stem without runners, densely pilose peduncle and twisted corolla lobes with ciliate margins.
Phallus aureus sp. nov. (Phallaceae, Basidiomycota) from Yunnan Province, China
Authors: LV TONG , LI YONG-RUI , AO CHENG-CE , HE JUN , YU FENG-MING , TANG SONG-MING , LI SHU-HONG
Published: September 2022

Phallus aureus is described as a new species from China and is characterized by an obovate, rugose, squamulose on the immature basidiomata surface, pseudostipe yellowish, grey volva, and pale yellow at the base becoming light yellow upwards. The new species is phylogenetically located in an independent lineage. A full description, colour photographs, illustrations, and a phylogenetic tree to show the position of new species are provided.
Trichoglottis longifolia (Orchidaceae: Epidendroideae: Vandeae: Aeridinae), a new species from Sri Lanka
Authors: BANDARA CHAMPIKA , ATTHANAGODA ANUSHA GAYAN , BANDARA NADEESHA LEWKE , RANASINGHE BHANUKA , KUMAR PANKAJ
Published: September 2022

Trichoglottis longifolia Atthan., C. Bandara, N.L. Bandara & Kumar sp. nov. is described and illustrated as a new species from Sri Lanka. So far, the genus was represented by a single species in Sri Lanka, Trichoglottis tenera. The new species can be easily distinguished from T. tenera in having longer leaves, yellowish-green flowers with lanceolate dorsal sepal and petals and a longer spur. This species occurs in sub-montane forests in Sabaragamuwa Province and categorized as Endangered based on the available data.
Renal histopathological lesions after liver transplantation: What can we find besides calcineurin inhibitor-induced nephrotoxicity?
Authors: Jin Haijiao , Wei Yuehan , Qian Yongbing , Zhang Jiang , Xu Yao , Zhou Hang , Zhang Minfang , Zhou Wenyan , Qi Chaojun , Jin Wei , Mou Shan , Wang Qin , Zhang Jianjun
Published: September 2022

Background

Chronic kidney disease (CKD) is a common complication after liver transplantation and is traditionally considered to be secondary to calcineurin inhibitors (CNIs). However, several studies have reported that the etiology of CKD after liver transplantation is broad and may only be assessed accurately by renal biopsy. The current study aimed to explore the usefulness of renal biopsies in managing CKD after liver transplantation in daily clinical practice.

Method

This retrospective analysis enrolled all post-liver transplantation patients who had a renal biopsy in a single center from July 2018 to February 2021.

Results

Fourteen renal biopsies were retrieved for review from 14 patients at a median of 35.7 (minimum-maximum: 2.80–134.73) months following liver transplantation. The male-to-female ratio was 13:1 (age range, 31–75 years). The histomorphological alterations were varied. The predominant glomerular histomorphological changes included focal segmental glomerular sclerosis (FSGS) (n = 4), diabetic glomerulopathy (n = 4), and membranoproliferative glomerulonephritis (n = 4). Thirteen (92.9%) patients had renal arteriolar sclerosis. Immune complex nephritis was present in six patients, of whom only two had abnormal serum immunological indicators. Despite interstitial fibrosis and tubular atrophy being present in all the patients, only six (42.9%) presented with severe interstitial injury. No major renal biopsy-related complications occurred. After a mean follow-up of 11.8 months (range: 1.2–29.8), three patients progressed to end-stage renal disease (ESRD).

Conclusion

The etiology of CKD after liver transplantation might be more complex than originally thought and should not be diagnosed simply as calcineurin inhibitors(CNI)-related nephropathy. Renal biopsy plays a potentially important role in the diagnosis and treatment of CKD after liver transplantation and might not be fully substituted by urine or blood tests. It may help avoid unnecessary changes to the immunosuppressants and inadequate treatment of primary diseases.
Comparison of effect of wobble board training with and without cognitive intervention on balance, ankle proprioception and jump landing kinetic parameters of men with chronic ankle instability: a randomized control trial
Authors: Taghavi Asl Abed , Shojaedin Seyed Sadredin , Hadadnezhad Malihe
Published: September 2022

Background

The aim of the present study was to compare the effect of a wobble board training (WBT) course with and without cognitive intervention (CI) on balance, ankle proprioception and jump-landing kinetic parameters in active men with chronic ankle instability (CAI).

Methods

Twenty-one active men in the age range of 18–25 years with CAI were randomly divided into three groups of WBT for five weeks as follows: with CI (n = 7); without CI (n = 7); control (n = 7). In this study, balance was evaluated by Y-Balance Test and Balance Error Scoring System, proprioception was evaluated as active and passive replication of 15-degree inversion angle and Maximum inversion angle minus 5-degrees, and single-leg jump-landing test was used for measurement of kinetic variables as pre-test and post-test in similar conditions.

Results

Post-test of both groups of WBT with and without CI showed a significant improvement in all variables compared to pre-test (p ≤ 0.05); however, in post-test, there was no significant difference in any of the variables between WBT groups with and without CI (p ≥ 0.05).

Conclusions

A course of WBT with and without CI leads to a significant improvement in balance, proprioception and jump-landing kinetic parameters, including time to stabilization and center of pressure displacement in active men with CAI. Although the WBT group with CI showed a greater improvement in mean than WBT group without CI, but the difference was not significant in any of the variables.

Trial registration

Retrospectively Registered. irict.ir Identifier: IRCT20200617047818N1 on 05–02-2021.
Prevalence, determinants and outcomes of traditional, complementary and alternative medicine use for hypertension among low-income households in Malaysia and the Philippines
Authors: Palileo-Villanueva Lia M. , Palafox Benjamin , Amit Arianna Maever L. , Pepito Veincent Christian F. , Ab-Majid Fadhlina , Ariffin Farnaza , Balabanova Dina , Isa Mohamad-Rodi , Mat-Nasir Nafiza , My Mazapuspavina , Renedo Alicia , Seguin Maureen L. , Yusoff Khalid , Dans Antonio L. , Mckee Martin
Published: September 2022

Background

Traditional, complementary and alternative medicine (TCAM) is used to treat a broad range of conditions. In low- and middle-income countries (LMICs), TCAM use is particularly common among those with low socio-economic status. To better understand the patterns and impact of TCAM use on the management of non-communicable diseases in these populations, this study examines the prevalence and characteristics of TCAM use for hypertension, its determinants, and its association with hypertension management outcomes and wellbeing among low-income adults in two Southeast Asian countries at different levels of economic and health system development, Malaysia and the Philippines.

Methods

We analysed cross-sectional data from 946 randomly selected adults diagnosed with hypertension from low-income rural and urban communities in Malaysia (n = 495) and the Philippines (n = 451). We compared the prevalence, characteristics and household expenditure on TCAM use between countries and used multi-level, mixed-effects regression to estimate associations between TCAM use and its determinants, and five hypertension management outcomes and wellbeing.

Results

The prevalence of TCAM use to manage hypertension was higher in the Philippines than in Malaysia (18.8% vs 8.8%,p < 0.001). Biologically-based modalities, e.g. herbal remedies, were the most common type of TCAM used in both countries, mainly as a complement, rather than an alternative to conventional treatment. Households allocated around 10% of health spending to TCAM in both countries. Belief that TCAM is effective for hypertension was a positive predictor of TCAM use, while belief in conventional medicine was a negative predictor. TCAM use was not strongly associated with current use of medications for hypertension, self-reported medication adherence, blood pressure level and control, or wellbeing in either country.

Conclusions

A small, but significant, proportion of individuals living in low-income communities in Malaysia and the Philippines use TCAM to manage their hypertension, despite a general lack of evidence on efficacy and safety of commonly used TCAM modalities. Recognising that their patients may be using TCAM to manage hypertension will enable health care providers to deliver safer, more patient-centred care.
Risk factors for level V metastasis in patients with N1b papillary thyroid cancer
Authors: Kang Jin Gu , Choi Jung Eun , Kang Su Hwan
Published: September 2022

Background

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer, and its incidence has increased. Lateral lymph node metastasis (LLNM) implies a worse prognosis than central lymph node metastasis, with a higher recurrence rate and decreased disease-free survival. The 2015 American Thyroid Association guidelines recommend compartmental node dissection in patients with LLNM to reduce the risk of recurrence and mortality. The purpose of this study was to identify the risk factors for level V lymph node (LN) metastasis in patients with N1b papillary thyroid cancer (PTC).

Methods

A total of 110 consecutive patients who underwent total thyroidectomy with lateral neck dissection for PTC between April 2016 and April 2022 were retrospectively enrolled. Based on level V metastasis, 94 patients were divided into two groups, and their clinicopathological characteristics were compared. Univariable analysis were used to assess the factors associated with level V metastasis. Spearman correlation analysis were used to assess the correlation between tumors and LN. The receiver operating characteristic (ROC) curve was used to determine the optimal cutoff value for the number of metastatic LNs at each level for level V metastasis.

Results

The number of metastatic LNs and lymph node ratio (LNR) in level II were significantly associated with level V metastasis (P = 0.011 and 0.001, respectively). The number of metastatic LNs in level II and those in the total number of levels correlated with the number of metastatic LNs in level V (rho = 0.331, 0.325, and P = 0.001, 0.001, respectively). The cutoff value for the number of metastatic LNs in level II was defined as 2.5 (area under the curve = 0.757, sensitivity = 50%, specificity = 82.5%, 95% confidence interval [CI] 0.626–0.889, P = 0.002). Simultaneous 3-level metastasis (level II, III, and IV) and 3-level with ≥ 2.5 metastatic LNs in level II were significantly associated with level V metastasis (P = 0.003 and 0.002).

Conclusions

The number of metastatic LNs and LNR in level II, simultaneous 3-level metastasis (level II, III, and IV), and 3-level with ≥ 2.5 metastatic LNs in level II were significantly associated with level V metastasis. (P = 0.011, 0.001, 0.003, and 0.002, respectively). In the future, larger-scale multi-institutional studies were needed to find out predictors for level V metastasis.
Establishment and application of a CRISPR-Cas12a-based RPA-LFS and fluorescence for the detection of Trichomonas vaginalis
Authors: Li Shan , Wang Xiaocen , Yu Yanhui , Cao Songgao , Liu Juan , Zhao Panpan , Li Jianhua , Zhang Xichen , Li Xin , Zhang Nan , Sun Min , Cao Lili , Gong Pengtao
Published: September 2022

Background

Infection with Trichomonas vaginalis can lead to cervicitis, urethritis, pelvic inflammatory disease, prostatitis and perinatal complications and increased risk of HIV transmission. Here, we used an RPA-based CRISPR-Cas12a assay system in combination with a lateral flow strip (LFS) (referred to as RPA-CRISPR-Cas12a) to establish a highly sensitive and field-ready assay and evaluated its ability to detect clinical samples.

Methods

We developed a one-pot CRISPR-Cas12a combined with RPA-based field detection technology for T. vaginalis, chose actin as the target gene to design crRNA and designed RPA primers based on the crRNA binding site. The specificity of the method was demonstrated by detecting genomes from nine pathogens. To improve the usability and visualize the RPA-CRISPR-Cas12a assay results, both fluorescence detection and LFS readouts were devised.

Results

The RPA-CRISPR-Cas12a assay platform was completed within 60 min and had a maximum detection limit of 1 copy/µl and no cross-reactivity with Candida albicans, Mycoplasma hominis, Neisseria gonorrhoeae, Escherichia coli, Cryptosporidium parvum, G. duodenalis or Toxoplasma gondii after specificity validation. Thirty human vaginal secretions were tested by RPA-CRISPR-Cas12a assays, and the results were read by a fluorescent reporter and LFS biosensors and then compared to the results from nested PCR detection of these samples. Both RPA-CRISPR-Cas12a assays showed 26.7% (8/30) T. vaginalis-positive samples and a consistency of 100% (8/8). The RPA-CRISPR-Cas12a assays had a higher sensitivity than nested PCR (only seven T. vaginalis-positive samples were detected).

Conclusions

The T. vaginalis RPA-CRISPR-Cas12a assay platform in this study can be used for large-scale field testing and on-site tests without the need for trained technicians or costly ancillary equipment.

Graphical abstract
Association of fecal calprotectin level with eosinophilic gastrointestinal disease in Iranian pediatrics
Authors: Rohani Pejman , Beheshti Narjes Raja , Alimadadi Hosein , Sohouli Mohammad Hassan
Published: September 2022

Introduction

Fecal calprotectin (FC) is a noninvasive biomarker for assessing the inflammatory status of the gastrointestinal tract. The aim of this study was to determine the association between FC levels and Eosinophilic colitis (EC) before and after treatment in pediatrics.

Method

In this cross-sectional study, 330 patients with rectorrhagia and FC levels > 200 μg/g were included in the study. Patients were then subjected to colonoscopy, and if 30 or more eosinophils were observed in the pathology of at least two parts of the colon, EC was diagnosed. Of the 330 patients included in the study, 14 patients were diagnosed as EC. Treatment included seven food elimination diet (food allergens) for 3 months. After 3 months, FC levels were repeated and colonoscopy was performed.

Results

The mean age of the children was 5.9 years. After the elimination diet, the number of eosinophils in all segments of colon significantly decreased (P < 0.001) and according to the pathology report, the number of eosinophils improved in 42.9% of patients. Also, the mean number of segments involved in the colon of patients was significantly decreased (P < 0.001). Mean FC levels were significantly decreased after 3 months (P < 0.001). The cut-off point of 114 μg/g of FC had sensitivity (75%), specificity (67%), positive predictive value (75%), negative predictive value (67%), accuracy (71.4%), and area under the ROC curve (0.708) acceptable in predicting EC.

Conclusion

This study showed that FC levels can be elevated in patients with EC, which is easily corrected with a targeted elimination of food allergens.