bluuebunny/crossref_metadata_2025 huggingface dataset:
Seismic Identification of Unconventional Heterogenous Reservoirs Based on Depositional History—A Case Study of the Polish Carpathian Foredeep
Authors: Łaba-Biel Anna , Kwietniak Anna , Urbaniec Andrzej
Published: November 2020

An integrated geological and geophysical approach is presented for the recognition of unconventional targets in the Miocene formations of the Carpathian Foredeep, southern Poland. The subject of the analysis is an unconventional reservoir built of interlayered packets of sandstone, mudstone and claystone, called a “heterogeneous sequence”. This type of sequence acts as both a reservoir and as source rock for hydrocarbons and it consists of layers of insignificant thickness, below the resolution of seismic data. The interpretation of such a sequence has rarely been based on seismic stratigraphy analysis; however, such an approach is proposed here. The subject of interpretation is high-quality seismic data of high resolution that enable detailed depositional analysis. The reconstruction of the depositional history was possible due to the analysis of flattened chronostratigraphic horizons (Wheeler diagram). The identification of depositional positions in a sedimentary basin was the first step for the indication of potential target areas. These areas were also subject to seismic attribute analysis (sweetness) and spectral decomposition. The seismic attribute results positively verified the previously proposed prospects. The results obtained demonstrate that the interpretation of the Miocene sediments in the Carpathian Foredeep should take into account the depositional history reconstruction and paleogeographical analysis.
Adoption of Energy Efficiency Measures in Renovation of Single-Family Houses: A Comparative Approach
Authors: Azizi Shoaib , Nair Gireesh , Olofsson Thomas
Published: November 2020

Inclusion of energy efficiency measures (EEMs) in the renovation of the single-family housing stock can unlock the potential for much-needed energy efficiency to tackle climate change. Energy renovation (ER) in single-family houses is often promoted as an aggregate process, and EEMs are treated homogenously without sufficient attention to their differences. This study applies a comparative analysis on common EEMs using chi-square test to investigate the influence of factors already found affecting the implementation of ER. This paper addresses the “personal” and “house-related” factors influencing the adoption of EEMs regardless of motives or barriers leading the adopters’ decisions. This strategy is useful to highlight the contexts leading to an increase in the adoption rate of different EEMs. The analysis is based on a questionnaire survey mailed in spring 2017 to 1550 single-family homeowners in the northern region of Sweden. Approximately 60% of respondents showed interest in adopting at least one EEM if they implement a major renovation. About 46% of respondents stated to have at least one indoor environmental problem (IEP) in their houses, and IEPs are found to have significant relations with homeowners’ interest to adopt several different EEMs. The policy implications related to different EEMs are discussed.
Mechanisms of Rock Burst in Horizontal Section Mining of a Steeply Inclined Extra-Thick Coal Seam and Prevention Technology
Authors: Cao Jinrong , Dou Linming , Zhu Guangan , He Jiang , Wang Shengchuan , Zhou Kunyou
Published: November 2020

Rock bursts have recently become a serious problem in the horizontal section mining of steeply inclined extra-thick coal seams (SIETCSs). However, few studies have been carried out to investigate their mechanisms and prevention. In this study, numerical simulation and field measurements were carried out to investigate the mechanism of rock bursts in the horizontal section mining of an SIETCS. A Universal Distinct Element Code (UDEC) Trigon model was built, based on the Yaojie No.3 Coal Mine, and calibrated through laboratory tests and RQD methods. The results demonstrate that the coal in the elastic zone around the roof is in a high static stress state, due to the asymmetric clamping and squeezing of the roof and floor. Strong dynamic loads are formed by breakage of the roof and the failure of multiple hinged beam structures during the evolution process of the overlying strata. Rock bursts occur on the roof side when the superimposition of the static stress σs and stress increment σd induced by such dynamic loads is greater than the critical stress σmin of the coal and rock. We propose a technical prevention scheme for the considered mine. Field studies suggest that the proposed technology can effectively prevent and control rock bursts in the horizontal section mining of SIETCSs.
Analyzing COVID-19 Impacts on Vehicle Travels and Daily Nitrogen Dioxide (NO2) Levels among Florida Counties
Authors: Karaer Alican , Balafkan Nozhan , Gazzea Michele , Arghandeh Reza , Ozguven Eren Erman
Published: November 2020

The COVID-19 outbreak and ensuing social distancing behaviors resulted in substantial reduction on traffic, making this a unique experiment on observing the air quality. Such an experiment is also supplemental to the smart city concept as it can help to identify whether there is a delay on air quality improvement during or after a sharp decline on traffic and to determine what, if any, factors are contributing to that time lag. As such, this study investigates the immediate impacts of COVID-19 causing abrupt declines on traffic and NO2 concentration in all Florida Counties through March 2020. Daily tropospheric NO2 concentrations were extracted from the Sentinel-5 Precursor satellite and vehicle mile traveled (VMT) estimates were acquired from cell phone mobility records. It is observed that overall impacts of the COVID-19 response in Florida have started in the first half of the March 2020, two weeks earlier than the official stay-at-home orders, and resulted in 54.07% and 59.68% decrease by the end of the month on NO2 and VMT, respectively. Further, a cross-correlation based dependency analysis was conducted to analyze the similarities and associated time lag between 7-day moving averages of VMT and NO2 concentrations of the 67 counties. Although such reduction is unprecedented for both data sets, results indicate a strong correlation and this correlation increases with the identification of a time lag between VMT and NO2 concentration. Majority of the counties have no time lag between VMT and NO2 concentration; however, a cluster of South Florida counties presents earlier decrease on NO2 concentration compare to VMT, which indicates that the air quality improvements in those counties are not traffic related. Investigation on the socioeconomic factors indicates that population density and income level have no significant impact on the time lag between traffic and air quality improvements in light of COVID-19.
Two-Stage Optimal Microgrid Operation with a Risk-Based Hybrid Demand Response Program Considering Uncertainty
Authors: Ryu Ho-Sung , Kim Mun-Kyeom
Published: November 2020

Owing to the increasing utilization of renewable energy resources, distributed energy resources (DERs) become inevitably uncertain, and microgrid operators have difficulty in operating the power systems because of this uncertainty. In this study, we propose a two-stage optimization approach with a hybrid demand response program (DRP) considering a risk index for microgrids (MGs) under uncertainty. The risk-based hybrid DRP is presented to reduce both operational costs and uncertainty effect using demand response elasticity. The problem is formulated as a two-stage optimization that considers not only the expected operation costs but also risk expense of uncertainty. To address the optimization problem, an improved multi-layer artificial bee colony (IML-ABC) is incorporated into the MG operation. The effectiveness of the proposed approach is demonstrated through a numerical analysis based on a typical low-voltage grid-connected MG. As a result, the proposed approach can reduce the operation costs which are taken into account uncertainty in MG. Therefore, the two-stage optimal operation considering uncertainty has been sufficiently helpful for microgrid operators (MGOs) to make risk-based decisions.
A Distribution System State Estimator Based on an Extended Kalman Filter Enhanced with a Prior Evaluation of Power Injections at Unmonitored Buses
Authors: Macii David , Fontanelli Daniele , Barchi Grazia
Published: November 2020

In the context of smart grids, Distribution Systems State Estimation (DSSE) is notoriously problematic because of the scarcity of available measurement points and the lack of real-time information on loads. The scarcity of measurement data influences on the effectiveness and applicability of dynamic estimators like the Kalman filters. However, if an Extended Kalman Filter (EKF) resulting from the linearization of the power flow equations is complemented by an ancillary prior least-squares estimation of the weekly active and reactive power injection variations at all buses, significant performance improvements can be achieved. Extensive simulation results obtained assuming to deploy an increasing number of next-generation smart meters and Phasor Measurement Units (PMUs) show that not only the proposed approach is generally more accurate and precise than the classic Weighted Least Squares (WLS) estimator (chosen as a benchmark algorithm), but it is also less sensitive to both the number and the metrological features of the PMUs. Thus, low-uncertainty state estimates can be obtained even though fewer and cheaper measurement devices are used.
Biogas Plant Exploitation in a Middle-Sized Dairy Farm in Poland: Energetic and Economic Aspects
Authors: Pochwatka Patrycja , Kowalczyk-Juśko Alina , Sołowiej Piotr , Wawrzyniak Agnieszka , Dach Jacek
Published: November 2020

Although cow manure is a valuable natural fertilizer, it is also a source of extreme greenhouse gas emissions, mainly methane. For this reason, this study aims to determine the impact of investments in a biogas plant on the energy and economic aspects of the operation of a dairy farm. A farm with a breeding size of 600 livestock units (LSU) was adopted for the analysis. In order to reach the paper’s aim, the analysis of two different scenarios of dairy farm functioning (conventional–only milk production, and modern–with biogas plant exploitation) was conducted. The analysis showed that the investment in biogas plant operations at a dairy farm and in using cow manure as one of the main substrates is a more profitable scenario compared to traditional dairy farming. Taking into account the actual Polish subsidies for electricity produced by small biogas plants, the scenario with a functioning biogas plant with a capacity of 500 kW brings €332,000/a more profit compared to the conventional scenario, even when taking into account additional costs, including the purchase of straw to ensure a continuous operation of the installation. Besides, in the traditional scenario, building a biogas plant allows for an almost complete reduction of greenhouse gas emissions during manure storage.
Weight Calculation Alternative Methods in Prime’s Algorithm Dedicated for Power System Restoration Strategies
Authors: Łukaszewski Artur , Nogal Łukasz , Robak Sylwester
Published: November 2020

In self-healing grid systems, high utility in the use of greedy algorithms is observed. One of the most popular solutions is based on Prim’s algorithm. In the computation, the power grid is represented as a weighted graph. This paper presents a few possibilities of calculation of the numerical weight of a branch of the graph. The proposition of a modified edge weight calculation based on active power belongs to this group. The other solutions are novel subalgorithms bounded by real power, reactive power, and normalized factor. This factor is a mathematical combination of active and reactive power multiplied by influence coefficients. Requirements necessary for a power system are applied in the considered algorithms. Each of these proposed algorithms includes the power source limits, voltage level at busbars, and power system transmission features, such as transmission lines rated currents and power losses. All mentioned methods were compiled into separate algorithms, which can be used to compute graph model parameters. A simulation model based on Prim’s algorithm was prepared to compare the suitability of presented concepts. All weights of the subalgorithms were compared to each other. That is why different power system restoration strategies may require various methods of calculating weights of the graph’s branches.
Application of Equilibrium Optimizer Algorithm for Optimal Power Flow with High Penetration of Renewable Energy
Authors: Nusair Khaled , Alhmoud Lina
Published: November 2020

In recent decades, the energy market around the world has been reshaped to accommodate the high penetration of renewable energy resources. Although renewable energy sources have brought various benefits, including low operation cost of wind and solar PV power plants, and reducing the environmental risks associated with the conventional power resources, they have imposed a wide range of difficulties in power system planning and operation. Naturally, classical optimal power flow (OPF) is a nonlinear problem. Integrating renewable energy resources with conventional thermal power generators escalates the difficulty of the OPF problem due to the uncertain and intermittent nature of these resources. To address the complexity associated with the process of the integration of renewable energy resources into the classical electric power systems, two probability distribution functions (Weibull and lognormal) are used to forecast the voltaic power output of wind and solar photovoltaic, respectively. Optimal power flow, including renewable energy, is formulated as a single-objective and multi-objective problem in which many objective functions are considered, such as minimizing the fuel cost, emission, real power loss, and voltage deviation. Real power generation, bus voltage, load tap changers ratios, and shunt compensators values are optimized under various power systems’ constraints. This paper aims to solve the OPF problem and examines the effect of renewable energy resources on the above-mentioned objective functions. A combined model of wind integrated IEEE 30-bus system, solar PV integrated IEEE 30-bus system, and hybrid wind and solar PV integrated IEEE 30-bus system is performed using the equilibrium optimizer technique (EO) and other five heuristic search methods. A comparison of simulation and statistical results of EO with other optimization techniques showed that EO is more effective and superior and provides the lowest optimization value in term of electric power generation, real power loss, emission index and voltage deviation.
Stall Mode Transformation in the Wide Vaneless Diffuser of Centrifugal Compressors
Authors: Heng Yaguang , Hu Bo , Jiang Qifeng , Wang Zhengwei , Liu Xiaobing
Published: November 2020

A review on the rotating stall in the vaneless diffuser of centrifugal compressors is presented showing that different stall modes characterized by different numbers of cells can be detected within the diffuser even if the operating condition remains unchanged. The interaction between the inlet perturbation and the stall cells near the diffuser outlet is supposed to be the trigger of the stall mode transformation. In order to determine if the inlet perturbation will interact with the downstream stall cells, a characteristic time analysis is proposed to estimate the characteristic time of the perturbation in radial and tangential directions. An additional theoretical model which focused on the development of the vaneless diffuser rotating stall is presented to determine the propagation velocity of the cells. The comparison between the characteristic time in two directions shows that one stall mode is able to evolve into another stall mode if a critical condition is met, and the stall mode transformation is more likely to start from a mode with a higher number of cells and is more likely to occur in the diffuser with a large radius ratio. Experimental results are also employed to validate the proposed critical condition, and good agreements are obtained.
The Influence of Seasonal Meteorology on Vehicle Exhaust PM2.5 in the State of California: A Hybrid Approach Based on Artificial Neural Network and Spatial Analysis
Authors: Yu Fan , Mohebbi Amin , Cai Shiqing , Akbariyeh Simin , Russo Brendan J. , Smaglik Edward J.
Published: November 2020

This study aims to develop a hybrid approach based on backpropagation artificial neural network (ANN) and spatial analysis techniques to predict particulate matter of size 2.5 µm (PM2.5) from vehicle exhaust emissions in the State of California using aerosol optical depth (AOD) and several meteorological indicators (relative humidity, temperature, precipitation, and wind speed). The PM2.5 data were generated using the Motor Vehicle Emission Simulator (MOVES). The measured meteorological variables and AOD were obtained from the California Irrigation Management Information System (CIMIS) and NASA’s Moderate Resolution Spectroradiometer (MODIS), respectively. The data were resampled to a seasonal format and downscaled over grids of 10 by 10 to 150 by 150. Coefficient of determination (R2), mean absolute percentage error (MAPE), and root mean square error (RMSE) were used to assess the quality of the ANN prediction model. The model peaked at winter seasons with R2 = 0.984, RMSE = 0.027, and MAPE = 25.311, whereas it had the lowest performance in summer with R2 = 0.920, RMSE = 0.057, and MAPE = 65.214. These results indicate that the ANN model can reasonably predict the PM2.5 mass and can be used to forecast future trends.
Enhanced Antioxidant Capacity of Puffed Turmeric (Curcuma longa L.) by High Hydrostatic Pressure Extraction (HHPE) of Bioactive Compounds
Authors: Choi Yohan , Kim Wooki , Lee Joo-Sung , Youn So Jung , Lee Hyungjae , Baik Moo-Yeol
Published: November 2020

Turmeric (Curcuma longa L.) is known for its health benefits. Several previous studies revealed that curcumin, the main active compound in turmeric, has antioxidant capacity. It has been previously demonstrated that puffing, the physical processing using high heat and pressure, of turmeric increases the antioxidant and anti-inflammatory activities by increasing phenolic compounds in the extract. The current study sought to determine if high hydrostatic pressure extraction (HHPE), a non-thermal extraction at over 100 MPa, aids in the chemical changes and antioxidant functioning of turmeric. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) analyses were conducted and assessed the content of total phenol compounds in the extract. The chemical changes of curcuminoids were also determined by high performance liquid chromatography (HPLC). Among the three variables of ethanol concentration, pressure level, and treatment time, ethanol concentration was the most influential factor for the HHPE of turmeric. HHPE at 400 MPa for 20 min with 70% EtOH was the optimal extraction condition for the highest antioxidant activity. Compositional analysis revealed that 2-methoxy-4-vinylphenol was produced by puffing. Vanillic acid and ferulic acid content increased with increasing HHPE time. Synergistic effect was not observed on antioxidant activity when the turmeric was sequentially processed using puffing and HHPE.
Nutritional and Functional Properties of Fermented Mixed Grains by Solid-State Fermentation with Bacillus amyloliquefaciens 245
Authors: Heo Su Jin , Kim Ah-Jin , Park Min-Ju , Kang Kimoon , Soung Do Yu
Published: November 2020

Fermented foods have several advantages, including increased nutritional value, improved bioavailability, and functional health properties. We examined that these outcomes were also observed in fermented mixed grains (FMG) containing wheat germ, wheat bran, oats, brown rice, barley, quinoa, and lentils following solid-state fermentation (SSF) by Bacillus amyloliquefaciens 245. The metabolic profile during fermentation was screened using capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS). The amino acids were quantitatively measured for the validation of the changes in metabolites. The activity of enzymes (e.g., amylase, protease, and fibrinolysis) and antioxidant capacity was also assessed to elucidate the functionality of FMG. The essential amino acid contents gradually increased as fermentation progressed. As the metabolites involved in the urea cycle and polyamine pathway were changed by fermentation, arginine was used as a substance to produce citrulline, ornithine, and agmatine. FMG showed dramatic increases in enzyme activity. FMG incubated for 36 h also displayed higher total phenolic contents and free radical scavenging ability than MG. The data suggest that FMG produced by Bacillus amyloliquefaciens 245 possess improved nutritional and functional quality, leading to their potential use as dietary supplements.
Stability and Antiglycoxidant Potential of Bilberry Anthocyanins in Simulated Gastrointestinal Tract Model
Authors: Fraisse Didier , Bred Alexis , Felgines Catherine , Senejoux François
Published: November 2020

Anthocyanins from Vaccinium myrtillus fruits have been reported in vitro to exert potent radical scavenging and antiglycation activities. However, the physiological relevance of such properties remains unclear given the potential susceptibility of anthocyanin derivatives to digestive conditions. A simulated gastrointestinal tract model was thus implemented to assess the impact of gastric and intestinal phases on the chemical integrity of bilberry anthocyanins and their antiglycoxidant effects. Results demonstrated that the investigated activities as well as total and individual anthocyanin contents were marginally affected by gastric conditions. By contrast, with recoveries ranging from 16.1 to 41.2%, bilberry anthocyanins were shown to be highly sensitive to the intestinal phase. Of major interest, a much better preservation was observed for radical scavenging and antiglycation activities as attested by recovery rates ranging from 79.1 to 86.7%. Consistently with previous observations, the present study confirms the moderate bioaccessibility of anthocyanin constituents. It does however provide valuable information supporting the persistence of substantial radical scavenging and antiglycation activities at each step of the digestion process. Taken together, these data indicate that digestive conditions might not abolish the potential positive effects of bilberry consumption on both oxidative and carbonyl stresses.
Novel Biodegradable Starch Film for Food Packaging with Antimicrobial Chicory Root Extract and Phytic Acid as a Cross-Linking Agent
Authors: Jaśkiewicz Andrzej , Budryn Grażyna , Nowak Agnieszka , Efenberger-Szmechtyk Magdalena
Published: November 2020

The aim of the study was to obtain and evaluate the properties of biodegradable starch film with the addition of phytic acid (0.05%) as a cross-linking agent and chicory root extract (1–5%) as an antimicrobial agent. To prepare biodegradable film, extracts from chicory root obtained with water or methanol were used. The content of bioactive compounds (sesquiterpene lactones and total polyphenols) was evaluated in chicory extracts. The antibacterial activity of the extracts was tested against Gram-negative bacteria (Pseudomonas fluorescens, Escherichia coli) and Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) using the microculture method. The extracts acted as bacteriostatic agents, decreasing the growth rate (µmax), and extending the lag phase (tlag). The most sensitive bacterium in terms of film bacteriostatic activity was P. fluorescens; all extracts, irrespective of the solvent used, decreased its µmax value. S. aureus was the least sensitive. The obtained films were tested for their properties as food packaging (color, thickness, permeability, mechanical strength). Phytic acid improved the tensile strength and barrier properties of the films. The antimicrobial activity of the films was studied by the disk diffusion method against Gram-negative (P. fluorescens, E. coli) and Gram-positive (B. subtilis, S. aureus) bacteria, as well as fungi (Candida albicans, Aspergillus niger). The growth-inhibiting activity of each obtained film was observed for all tested microorganisms, and the most beneficial effect was observed for films with the 5% level of added extracts obtained with water. The growth-inhibiting activity for fungi, in particular for the yeast C. albicans, was low.
Nutrients and Pathways that Regulate Health Span and Life Span
Authors: Pignatti Carla , D’Adamo Stefania , Stefanelli Claudio , Flamigni Flavio , Cetrullo Silvia
Published: November 2020

Both life span and health span are influenced by genetic, environmental and lifestyle factors. With the genetic influence on human life span estimated to be about 20–25%, epigenetic changes play an important role in modulating individual health status and aging. Thus, a main part of life expectance and healthy aging is determined by dietary habits and nutritional factors. Excessive or restricted food consumption have direct effects on health status. Moreover, some dietary interventions including a reduced intake of dietary calories without malnutrition, or a restriction of specific dietary component may promote health benefits and decrease the incidence of aging-related comorbidities, thus representing intriguing potential approaches to improve healthy aging. However, the relationship between nutrition, health and aging is still not fully understood as well as the mechanisms by which nutrients and nutritional status may affect health span and longevity in model organisms. The broad effect of different nutritional conditions on health span and longevity occurs through multiple mechanisms that involve evolutionary conserved nutrient-sensing pathways in tissues and organs. These pathways interacting each other include the evolutionary conserved key regulators mammalian target of rapamycin, AMP-activated protein kinase, insulin/insulin-like growth factor 1 pathway and sirtuins. In this review we provide a summary of the main molecular mechanisms by which different nutritional conditions, i.e., specific nutrient abundance or restriction, may affect health span and life span.
Chemical Element Concentrations of Cycad Leaves: Do We Know Enough?
Authors: Deloso Benjamin E. , Krishnapillai Murukesan V. , Ferreras Ulysses F. , Lindström Anders J. , Calonje Michael , Marler Thomas E.
Published: November 2020

The literature containing which chemical elements are found in cycad leaves was reviewed to determine the range in values of concentrations reported for essential and beneficial elements. We found 46 of the 358 described cycad species had at least one element reported to date. The only genus that was missing from the data was Microcycas. Many of the species reports contained concentrations of one to several macronutrients and no other elements. The cycad leaves contained greater nitrogen and phosphorus concentrations than the reported means for plants throughout the world. Magnesium was identified as the macronutrient that has been least studied. Only 14 of the species were represented by data from in situ locations, with most of the data obtained from managed plants in botanic gardens. Leaf element concentrations were influenced by biotic factors such as plant size, leaf age, and leaflet position on the rachis. Leaf element concentrations were influenced by environmental factors such as incident light and soil nutrient concentrations within the root zone. These influential factors were missing from many of the reports, rendering the results ambiguous and comparisons among studies difficult. Future research should include the addition of more taxa, more in situ locations, the influence of season, and the influence of herbivory to more fully understand leaf nutrition for cycads.
‘Sex Is Not Just about Ovaries.’ Youth Participatory Research on Sexuality Education in The Netherlands
Authors: Cense Marianne , Grauw Steven de , Vermeulen Manouk
Published: November 2020

Young people are not satisfied with the sexuality education they receive in Dutch high schools. They rate their sexuality education as mediocre (5.8 on a scale of one to ten). In cooperation with 17 young peer researchers, we explored what good sexuality education looks like from the point of view of young people. The peer researchers collected data in their own high school, using mixed methods, starting with individual interviews, followed by focus group discussions and Photovoice sessions to get more in-depth views on topics, class atmosphere, and teacher skills. In total, 300 pupils aged 12–18 participated in the research. Our findings demonstrate that young people want more sexuality education, during their whole school career. They want sexuality education to move beyond biological functions, sexually transmitted diseases, and reproduction into issues like dating, online behavior, sexual pleasure, relationships, and sexual coercion. Moreover, pupils want sexual diversity integrated and normalized in all content. One of the main issues is that sexuality education should be given in a safe class atmosphere, which demands sensitivity from the teacher. In addition to the findings of the study, this article reflects on the steps to be taken to realize the changes desired by young people.
Prevalence of Type 2 Diabetes and Its Association with Added Sugar Intake in Citizens and Refugees Aged 40 or Older in the Gaza Strip, Palestine
Authors: Jebril Majed , Liu Xin , Shi Zumin , Mazidi Mohsen , Altaher Akram , Wang Youfa
Published: November 2020

Background: Little is known about the prevalence and risk factors of diabetes among Gaza Palestinians, 64% of whom are refugees with exceeded sugar intake. We aimed to estimate the prevalence of type 2 diabetes (T2D) and its association with added sugar intake among residents, with regular visits to primary healthcare centers (PHCs) across Gaza. Methods: From October to December of 2019, a cross-sectional survey was conducted among 1000 citizens and refugees in nine PHCs selected from the five governorates of the Gaza Strip. Information on dietary intake, medical history, and other risk factors was collected by trained health workers, using structured questionnaires. Anthropometry and biochemical data were extracted from the PHC medical record system. Results: Overall, the prevalence of diagnosed T2D and undiagnosed T2D were 45.2% and 16.8%, respectively, in adults aged 42 to 74 years, with the differences among citizens and refugees (diagnosed: 46.2% vs. 43.8%; undiagnosed: 15.7% vs. 18.2%). The uncontrolled glycaemic rate was 41.9% and 36.8% for diagnosed patients in citizens and refugees, respectively. Among those without a clinical diagnosis of T2D, after multivariable adjustment, daily added sugar intake was positively associated with fasting glucose and the risk of undiagnosed T2D (odds ratio, 95% CI, highest vs. lowest intake, was 2.71 (1.12–6.54) (pfor trend < 0.001). In stratified analysis, the associations between added sugar intake and the risk of undiagnosed T2D tend to be stronger among refugees or those with higher body mass index. Conclusions: Among Palestinian adults, both citizens and refugees are affected by T2D. Added sugar intake is associated with the risk of undiagnosed T2D.
Health Literacy and Preventive Behaviors Modify the Association between Pre-Existing Health Conditions and Suspected COVID-19 Symptoms: A Multi-Institutional Survey
Authors: Nguyen Tan T. , Le Nga T. , Nguyen Minh H. , Pham Linh V. , Do Binh N. , Nguyen Hoang C. , Nguyen Huu C. , Ha Tung H. , Dao Hung K. , Nguyen Phuoc B. , Trinh Manh V. , Do Thinh V. , Nguyen Hung Q. , Nguyen Thao T. P. , Nguyen Nhan P. T. , Tran Cuong Q. , Tran Khanh V. , Duong Trang T. , Pham Thu T. M. , Duong Tuyen Van
Published: November 2020

People with pre-existing health conditions (PEHC) are vulnerable to viral infection while health literacy (HL) and preventive behaviors (PB) have been shown to benefit people during the COVID-19 pandemic. The aim of this study was to examine the association between PEHC and suspected COVID-19 symptoms (S-COVID-19-S), and to investigate the modification effect of HL and PB. A cross-sectional study was conducted on 8291 participants visiting outpatient departments at 18 hospitals and health centers across Vietnam from 14 February to 31 May 2020. Data were collected regarding participant’s characteristics, HL, PB, PEHC, and S-COVID-19-S. Regression models were used for analyzing the associations. Results showed that people with PEHC had a 3.38 times higher likelihood of having S-COVID-19-S (odds ratio, OR, 3.38; 95% confidence interval, 95% CI, 3.01, 3.79; p < 0.001). In comparison to participants without PEHC and with the lowest HL score, those with PEHC and one HL score increment had a 7% lower likelihood of having S-COVID-19-S (OR, 0.93; 95% CI, 0.92, 0.94; p < 0.001). In comparison to participants without PEHC and not adhering to mask wearing, those with PEHC and adhering to mask wearing had a 77% lower likelihood of having S-COVID-19-S (OR, 0.23; 95% CI, 0.16, 0.32; p < 0.001). Higher HL and adherence to mask wearing can protect people from having S-COVID-19-S, especially in those with PEHC.
Exploring Evaluation Variables for Low-Cost Particulate Matter Monitors to Assess Occupational Exposure
Authors: Ruiter Sander , Kuijpers Eelco , Saunders John , Snawder John , Warren Nick , Gorce Jean-Philippe , Blom Marcus , Krone Tanja , Bard Delphine , Pronk Anjoeka , Cauda Emanuele
Published: November 2020

(1) Background: Small, lightweight, low-cost optical particulate matter (PM) monitors are becoming popular in the field of occupational exposure monitoring, because these devices allow for real-time static measurements to be collected at multiple locations throughout a work site as well as being used as wearables providing personal exposure estimates. Prior to deployment, devices should be evaluated to optimize and quantify measurement accuracy. However, this can turn out to be difficult, as no standardized methods are yet available and different deployments may require different evaluation procedures. To gain insight in the relevance of different variables that may affect the monitor readings, six PM monitors were selected based on current availability and evaluated in the laboratory; (2) Methods: Existing strategies that were judged appropriate for the evaluation of PM monitors were reviewed and seven evaluation variables were selected, namely the type of dust, within- and between-device variations, nature of the power supply, temperature, relative humidity, and exposure pattern (peak and constant). Each variable was tested and analyzed individually and, if found to affect the readings significantly, included in a final correction model specific to each monitor. Finally, the accuracy for each monitor after correction was calculated; (3) Results: The reference materials and exposure patterns were found to be main factors needing correction for most monitors. One PM monitor was found to be sufficiently accurate at concentrations up to 2000 µg/m3 PM2.5, with other monitors appropriate at lower concentrations. The average accuracy increased by up to three-fold compared to when the correction model did not include evaluation variables; (4) Conclusions: Laboratory evaluation and readings correction can greatly increase the accuracy of PM monitors and set boundaries for appropriate use. However, this requires identifying the relevant evaluation variables, which are heavily reliant on how the monitors are used in the workplace. This, together with the lack of current consensus on standardized procedures, shows the need for harmonized PM monitor evaluation methods for occupational exposure monitoring.
Baltic Adolescents’ Health Behaviour: An International Comparison
Authors: Oja Leila , Slapšinskaitė Agnė , Piksööt Jaanika , Šmigelskas Kastytis
Published: November 2020

The aim of the study is to assess the time trends in Baltic adolescents’ physical activity, dietary habits and BMI and compare the results with the average of the Health Behaviour in School-aged Children (HBSC) study. The research used HBSC data from 2006 to 2018. The total number of respondents was 17,458 in Estonia, 18,416 in Latvia and 20,466 in Lithuania. A logistic regression analysis was applied to estimate time trends in health behaviour indicators. The results demonstrated that Baltic adolescents’ physical activity has declined over the study years, except for Lithuanian girls. The prevalence of overweight adolescents has significantly increased since 2006. Dietary habits improved in all three Baltic countries, as consumption of vegetables increased, and soft drink consumption decreased during this time period. This research shows that a nationwide, highly representative study with health behaviour indicators enables us to assess regional differences compared to the HBSC average. The prevalence of overweight and obese adolescents in the Baltic countries has increased and moved closer to the HBSC average. Although Baltic adolescents’ daily vegetable consumption has increased over the last decade, it is still lower than the HBSC average.
The Impact of Financial Statement Comparability on Earnings Management: Evidence from Frontier Markets
Authors: Martens Wil , Yapa Prem W. S. , Safari Maryam
Published: November 2020

This paper examined whether financial statement comparability constrains opportunistic earnings management in frontier market countries. Using a large sample of 19 frontier market countries, and an accounting comparability method that maps comparability across several accounting standards, the results show that enhanced financial comparability constrains accruals earnings management (AEM). Contrary to developed markets and novel to this study, a significant relationship between financial comparability and real earnings management (REM) was not found. For greater robustness, AEM and REM were also tested on both International Financial Reporting Standards (IFRS) adopting and non-adopting countries. The results suggest IFRS adoption constrains AEM, yet exhibited no impact on constraining REM. Additionally, the use of BigN auditors failed to conclusively show an ability to moderate EM. When combined, the results suggest that frontier markets engage in less REM than expected. It is also noted that the legal roots (civil vs. common law) play a significant role in constraining earnings management. Common law countries exhibited lower AEM when comparability increased; this significance was not found in countries that were rooted in civil law. Contributions from this study show that findings from developed markets cannot be generalised to frontier markets.
Sulforaphane Reduces Prostate Cancer Cell Growth and Proliferation In Vitro by Modulating the Cdk-Cyclin Axis and Expression of the CD44 Variants 4, 5, and 7
Authors: Rutz Jochen , Thaler Sarah , Maxeiner Sebastian , Chun Felix K.-H. , Blaheta Roman A.
Published: November 2020

Prostate cancer patients whose tumors develop resistance to conventional treatment often turn to natural, plant-derived products, one of which is sulforaphane (SFN). This study was designed to determine whether anti-tumor properties of SFN, identified in other tumor entities, are also evident in cultivated DU145 and PC3 prostate cancer cells. The cells were incubated with SFN (1–20 µM) and tumor cell growth and proliferative activity were evaluated. Having found a considerable anti-growth, anti-proliferative, and anti-clonogenic influence of SFN on both prostate cancer cell lines, further investigation into possible mechanisms of action were performed by evaluating the cell cycle phases and cell-cycle-regulating proteins. SFN induced a cell cycle arrest at the S- and G2/M-phase in both DU145 and PC3 cells. Elevation of histone H3 and H4 acetylation was also evident in both cell lines following SFN exposure. However, alterations occurring in the Cdk-cyclin axis, modification of the p19 and p27 proteins and changes in CD44v4, v5, and v7 expression because of SFN exposure differed in the two cell lines. SFN, therefore, does exert anti-tumor properties on these two prostate cancer cell lines by histone acetylation and altering the intracellular signaling cascade, but not through the same molecular mechanisms.
Network Analysis of Gut Microbiome and Metabolome to Discover Microbiota-Linked Biomarkers in Patients Affected by Non-Small Cell Lung Cancer
Authors: Vernocchi Pamela , Gili Tommaso , Conte Federica , Del Chierico Federica , Conta Giorgia , Miccheli Alfredo , Botticelli Andrea , Paci Paola , Caldarelli Guido , Nuti Marianna , Marchetti Paolo , Putignani Lorenza
Published: November 2020

Several studies in recent times have linked gut microbiome (GM) diversity to the pathogenesis of cancer and its role in disease progression through immune response, inflammation and metabolism modulation. This study focused on the use of network analysis and weighted gene co-expression network analysis (WGCNA) to identify the biological interaction between the gut ecosystem and its metabolites that could impact the immunotherapy response in non-small cell lung cancer (NSCLC) patients undergoing second-line treatment with anti-PD1. Metabolomic data were merged with operational taxonomic units (OTUs) from 16S RNA-targeted metagenomics and classified by chemometric models. The traits considered for the analyses were: (i) condition: disease or control (CTRLs), and (ii) treatment: responder (R) or non-responder (NR). Network analysis indicated that indole and its derivatives, aldehydes and alcohols could play a signaling role in GM functionality. WGCNA generated, instead, strong correlations between short-chain fatty acids (SCFAs) and a healthy GM. Furthermore, commensal bacteria such as Akkermansia muciniphila, Rikenellaceae, Bacteroides, Peptostreptococcaceae, Mogibacteriaceae and Clostridiaceae were found to be more abundant in CTRLs than in NSCLC patients. Our preliminary study demonstrates that the discovery of microbiota-linked biomarkers could provide an indication on the road towards personalized management of NSCLC patients.
Suppression of Non-Random Fertilization by MHC Class I Antigens
Authors: Kamiya Junki , Kang Woojin , Yoshida Keiichi , Takagi Ryota , Kanai Seiya , Hanai Maito , Nakamura Akihiro , Yamada Mitsutoshi , Miyamoto Yoshitaka , Miyado Mami , Kuroki Yoko , Hayashi Yoshiki , Umezawa Akihiro , Kawano Natsuko , Miyado Kenji
Published: November 2020

Hermaphroditic invertebrates and plants have a self-recognition system on the cell surface of sperm and eggs, which prevents their self-fusion and enhances non-self-fusion, thereby contributing to genetic variation. However, the system of sperm–egg recognition in mammals is under debate. To address this issue, we explored the role of major histocompatibility complex class I (MHC class I, also known as histocompatibility 2-Kb or H2-Kb and H2-Db in mice) antigens by analyzing H2-Kb-/-H2-Db-/-β2-microglobulin (β2M)-/- triple-knockout (T-KO) male mice with full fertility. T-KO sperm exhibited an increased sperm number in the perivitelline space of wild-type (WT) eggs in vitro. Moreover, T-KO sperm showed multiple fusion with zona pellucida (ZP)-free WT eggs, implying that the ability of polyspermy block for sperm from T-KO males was weakened in WT eggs. When T-KO male mice were intercrossed with WT female mice, the percentage of females in progeny increased. We speculate that WT eggs prefer fusion with T-KO sperm, more specifically X-chromosome-bearing sperm (X sperm), suggesting the presence of preferential (non-random) fertilization in mammals, including humans.
Role of iRhoms 1 and 2 in Endochondral Ossification
Authors: Fang Renpeng , Haxaire Coline , Otero Miguel , Lessard Samantha , Weskamp Gisela , McIlwain David R. , Mak Tak W. , Lichtenthaler Stefan F. , Blobel Carl P.
Published: November 2020

Growth of the axial and appendicular skeleton depends on endochondral ossification, which is controlled by tightly regulated cell–cell interactions in the developing growth plates. Previous studies have uncovered an important role of a disintegrin and metalloprotease 17 (ADAM17) in the normal development of the mineralized zone of hypertrophic chondrocytes during endochondral ossification. ADAM17 regulates EGF-receptor signaling by cleaving EGFR-ligands such as TGFα from their membrane-anchored precursor. The activity of ADAM17 is controlled by two regulatory binding partners, the inactive Rhomboids 1 and 2 (iRhom1, 2), raising questions about their role in endochondral ossification. To address this question, we generated mice lacking iRhom2 (iR2−/−) with floxed alleles of iRhom1 that were specifically deleted in chondrocytes by Col2a1-Cre (iR1∆Ch). The resulting iR2−/−iR1∆Ch mice had retarded bone growth compared to iR2−/− mice, caused by a significantly expanded zone of hypertrophic mineralizing chondrocytes in the growth plate. Primary iR2−/−iR1∆Ch chondrocytes had strongly reduced shedding of TGFα and other ADAM17-dependent EGFR-ligands. The enlarged zone of mineralized hypertrophic chondrocytes in iR2−/−iR1∆Ch mice closely resembled the abnormal growth plate in A17∆Ch mice and was similar to growth plates in Tgfα−/− mice or mice with EGFR mutations. These data support a model in which iRhom1 and 2 regulate bone growth by controlling the ADAM17/TGFα/EGFR signaling axis during endochondral ossification.
Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome
Authors: Cano-Ortiz Antonio , Laborda-Illanes Aurora , Plaza-Andrades Isaac , Membrillo del Pozo Alberto , Villarrubia Cuadrado Alberto , Rodríguez Calvo de Mora Marina , Leiva-Gea Isabel , Sanchez-Alcoholado Lidia , Queipo-Ortuño María Isabel
Published: November 2020

The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren’s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation.