bluuebunny/crossref_metadata_2025 huggingface dataset:
Out-of-season spawning of largemouth bass in a controllable recirculating system
Authors: Hu Chen-Hao , Bie Han-Qing , Lu Zi-Yi , Ding Yang , Guan He-He , Geng Long-Hui , Ma Shuai , Hu Yuan-Xiang , Fan Qi-Xue , Shen Zhi-Gang
Published: April 2023

Largemouth bass (LMB) production exceeded 0.7 million tons in 2021 and has become one of the most important freshwater aquaculture species in China. The stable and fixed culture cycle led to regular and drastic price fluctuation during the past decade. Strong price fluctuation provides opportunities and challenges for the LMB industry, and out-of-season spawning (OSS) and culture will provide technical support for the opportunities. To induce OSS at a low cost, we established a controllable recirculating system that allows precise thermo-photoperiod manipulation. In the system, four experimental groups were assigned, 18NP (18°C overwintering water temperature, natural photoperiod), 18CP (18°C overwintering water temperature, controlled photoperiod), 16CP (16°C overwintering water temperature, controlled photoperiod), and NTNP (natural water temperature and natural photoperiod), to determine the effects of chilling temperature and photoperiod on spawning performance. OSS was observed in all the experimental groups without significant differences, except NTNP. The manipulated broodstock can re-spawn 3 months later in the next spring in advance. Further analysis of the volume percentage of different stages of oocytes provides a base for excellent regression between the volume percentage of the primary growth stage, cortical alveoli stage, vitellogenesis/maturation stage, and gonadal development/maturation. The results suggest that the volume percentage of oocytes is a better indicator of gonadal development and maturation than the gonadosomatic index. We also found that LMB prefers palm fiber as a spawning nest over gravel. The findings of this work provide important technique guidance for practical OSS of the LMB aquaculture industry and standardization of ovary development and maturation in fish with asynchronous developmental oocytes.
Serial intravital 2-photon microscopy and analysis of the kidney using upright microscopes
Authors: Sardella Donato , Kristensen Anders M. , Bordoni Luca , Kidmose Hanne , Shahrokhtash Ali , Sutherland Duncan S. , Frische Sebastian , Schiessl Ina Maria
Published: April 2023

Serial intravital 2-photon microscopy of the kidney and other abdominal organs is a powerful technique to assess tissue function and structure simultaneously and over time. Thus, serial intravital microscopy can capture dynamic tissue changes during health and disease and holds great potential to characterize (patho-) physiological processes with subcellular resolution. However, successful image acquisition and analysis require significant expertise and impose multiple potential challenges. Abdominal organs are rhythmically displaced by breathing movements which hamper high-resolution imaging. Traditionally, kidney intravital imaging is performed on inverted microscopes where breathing movements are partly compensated by the weight of the animal pressing down. Here, we present a custom and easy-to-implement setup for intravital imaging of the kidney and other abdominal organs on upright microscopes. Furthermore, we provide image processing protocols and a new plugin for the free image analysis software FIJI to process multichannel fluorescence microscopy data. The proposed image processing pipelines cover multiple image denoising algorithms, sample drift correction using 2D registration, and alignment of serial imaging data collected over several weeks using landmark-based 3D registration. The provided tools aim to lower the barrier of entry to intravital microscopy of the kidney and are readily applicable by biomedical practitioners.
Mapping a leaf rust resistance gene LrOft in durum wheat Ofanto and its suppressor SuLrOft in common wheat
Authors: Zhuansun Xiangxi , Sun Junna , Liu Nannan , Zhang Shengnan , Wang Huifang , Hu Zhaorong , Ma Jun , Sun Qixin , Xie Chaojie
Published: April 2023

Epidemics of leaf rust (caused by the fungal pathogen Puccinia triticina Erikss., Pt) raise concerns regarding sustainability of wheat production. Deployment of resistant cultivars is the most effective and economic strategy for combating this disease. Ofanto is a durum wheat cultivar that exhibits high resistance to Pt race PHT throughout its entire growing period. In the present study, we identified a leaf rust resistance gene in Ofanto and temporarily designated it as LrOft. LrOft was mapped to a 2.5 cM genetic interval in chromosome arm 6BL between Indel markers 6B6941 and 6B50L24. During introgression of LrOft from Ofanto to common wheat it was observed that F1 plants of Ofanto crossed with Shi4185 exhibited leaf rust resistance whereas the F1 of Ofanto crossed with ND4503 was susceptible. In order to map the presumed suppressor locus, a Shi4185/ND4503//Ofanto three-way pentaploid population was generated and SuLrOft was mapped on chromosome arm 2AS. SuLrOft was mapped within a 2.6 cM genetic interval flanked by 2AS50L14 and 2AS50L6. Fine mapping using 2,268 plants of the three-way cross narrowed the suppressor locus to a 68.2-kbp physical interval according to IWGSC RefSeq v1.1. Sequence analysis of genes in the physical interval revealed that TraesCS2A02G110800 encoding an RPP-13-like protein with an NB-ARC domain was a potential candidate for SuLrOft.
Contributions of phenotypic integration, plasticity and genetic adaptation to adaptive capacity relating to drought in Banksia marginata (Proteaceae)
Authors: Oyanoghafo Osazee O. , Miller Adam D. , Toomey Madeline , Ahrens Collin W. , Tissue David T. , Rymer Paul D.
Published: April 2023

The frequency and intensity of drought events are predicted to increase because of climate change, threatening biodiversity and terrestrial ecosystems in many parts of the world. Drought has already led to declines in functionally important tree species, which are documented in dieback events, shifts in species distributions, local extinctions, and compromised ecosystem function. Understanding whether tree species possess the capacity to adapt to future drought conditions is a major conservation challenge. In this study, we assess the capacity of a functionally important plant species from south-eastern Australia (Banksia marginata, Proteaceae) to adapt to water-limited environments. A water-manipulated common garden experiment was used to test for phenotypic plasticity and genetic adaptation in seedlings sourced from seven provenances of contrasting climate-origins (wet and dry). We found evidence of local adaptation relating to plant growth investment strategies with populations from drier climate-origins showing greater growth in well-watered conditions. The results also revealed that environment drives variation in physiological (stomatal conductance, predawn and midday water potential) and structural traits (wood density, leaf dry matter content). Finally, these results indicate that traits are coordinated to optimize conservation of water under water-limited conditions and that trait coordination (phenotypic integration) does not constrain phenotypic plasticity. Overall, this study provides evidence for adaptive capacity relating to drought conditions in B. marginata, and a basis for predicting the response to climate change in this functionally important plant species.
The association of negative mood with automatic and effortful facial expression mimicry
Authors: Kraft-Feil Tara L. , Ingram Rick E. , Gorey Claire , Luu Jazlyn H. , Cross Marie P. , Pressman Sarah D.
Published: April 2023

The natural process of mimicking the facial expressions of others is well established, as are the deficits in this reflexive behavior for individuals with clinical disorders such as depression. This study examines the extent of this deficit in non-clinical individuals with high transient negative mood, and whether it extends to both automatic and effortful emotion expression behavior. One hundred and thirty-six participants were shown happy, sad, and neutral faces, while electromyography (EMG) recorded facial muscle responses. Automatic (reflexive) mimicry was assessed while participants simply viewed facially expressive photographs, while effortful mimicry was monitored when individuals were told to intentionally copy the expressions in the photographs. Results indicated that high levels of negative mood were primarily associated with deficits in effortful mimicry of happy expressions, although some similar evidence was found in automatic mimicry of happy faces. Surprisingly, there were also ties between negative moods and inaccuracies in effortful mimicry of sad expressions (but not automatic mimicry). Inaccurate automatic and effortful mimicry were also tied with lower self-reported social support and greater loneliness. These results indicate that even in healthy individuals, transient and minor changes in negative mood are tied to deficiencies in facial mimicry at both the automatic and effortful level.
Literacy acquisition facilitates inversion effects for faces with full-, low-, and high-spatial frequency: evidence from illiterate and literate adults
Authors: Yang Qi , Zhang Lina , Chen Changming , Cao Xiaohua
Published: April 2023

Previous studies have found that literacy acquisition modulates configural face processing (i.e., holistic and second-order configural processing). However, it remains unclear how literacy acquisition impacts the configural processing indexed by the inversion effect of normal or filtered faces. We asked Chinese illiterate and literate adults to judge whether two sequentially-presented stimuli, including faces, houses (experiment 1), and high- or low-pass filtered faces (experiment 2) were identical. Literate adults outperformed illiterate controls in the upright face and house conditions (experiment 1) and the upright high- and low-pass filtered conditions (experiment 2) but not in the inverted conditions. Notably, the size of an inversion effect (i.e., subtracting inverted accuracy from upright accuracy) was greater among literate adults than that among illiterate adults in both experiments. These findings support that literacy acquisition promotes configural face processing.
Optimizing two-dose vaccine resource allocation to combat a pandemic in the context of limited supply: The case of COVID-19
Authors: Zhu Jin , Wang Qing , Huang Min
Published: April 2023

The adequate vaccination is a promising solution to mitigate the enormous socio-economic costs of the ongoing COVID-19 pandemic and allow us to return to normal pre-pandemic activity patterns. However, the vaccine supply shortage will be inevitable during the early stage of the vaccine rollout. Public health authorities face a crucial challenge in allocating scarce vaccines to maximize the benefits of vaccination. In this paper, we study a multi-period two-dose vaccine allocation problem when the vaccine supply is highly limited. To address this problem, we constructed a novel age-structured compartmental model to capture COVID-19 transmission and formulated as a nonlinear programming (NLP) model to minimize the total number of deaths in the population. In the NLP model, we explicitly take into account the two-dose vaccination procedure and several important epidemiologic features of COVID-19, such as pre-symptomatic and asymptomatic transmission, as well as group heterogeneity in susceptibility, symptom rates, severity, etc. We validated the applicability of the proposed model using a real case of the 2021 COVID-19 vaccination campaign in the Midlands of England. We conducted comparative studies to demonstrate the superiority of our method. Our numerical results show that prioritizing the allocation of vaccine resources to older age groups is a robust strategy to prevent more subsequent deaths. In addition, we show that releasing more vaccine doses for first-dose recipients could lead to a greater vaccination benefit than holding back second doses. We also find that it is necessary to maintain appropriate non-pharmaceutical interventions (NPIs) during the vaccination rollout, especially in low-resource settings. Furthermore, our analysis indicates that starting vaccination as soon as possible is able to markedly alleviate the epidemic impact when the vaccine resources are limited but are currently available. Our model provides an effective tool to assist policymakers in developing adaptive COVID-19 likewise vaccination strategies for better preparedness against future pandemic threats.
Participatory action research to co-design a culturally appropriate COVID-19 risk communication and community engagement strategy in rural Pakistan
Authors: Moran Victoria Hall , Ceballos-Rasgado Marena , Fatima Sadia , Mahboob Usman , Ahmad Salman , McKeown Michael , Zaman Mukhtiar
Published: April 2023

Background

Community engagement has shown to be fundamental component of the response to previous disease outbreaks. This study aimed co-design and implement a culturally appropriate COVID-19 risk communication and community engagement strategy with a resource-poor rural community in Northwest Pakistan.

Methods

Participatory Action Research (PAR) was conducted from January 2021 to March 2022. Five PAR meetings took place with community members (n = 30) to: (1) explore how the COVID-19 pandemic impacted on the community; (2) identify challenges to limit the spread of the virus; (3) identify and implement solutions to these challenges; and (4) highlight the enablers, challenges and knowledge of the cultural context needed to optimize safety during emergencies. Focus group discussions (N = 6) with community members not involved in the PAR meetings (N = 50) and children of the community (N = 26) were conducted following the PAR meetings. Thematic analysis of the PAR and focus group data was conducted.

Results

Delivery of messages on how to keep families safe, provision of personal protective equipment and improved water systems were part of the strategies taken by the community to create awareness and reduce the spread of COVID-19. Nine themes were identified: Attitudes to the pandemic: From skepticism to acceptance, Changing attitudes about vaccination: rumors and trust, COVID-19 and Faith, Social impact of the pandemic, Access to water, Resource mobilization: personal protective equipment, Spaces where collaborative effort can bring to solutions, Agents of change, and Empowerment of women.

Discussion

The participatory approach of this research allowed understanding of the challenges faced by the community to engage in behavior change strategies to reduce the spread of COVID-19 and enabled the community to find sustainable solutions. Engagement with the community empowered men and women to be agents of change and promoted necessary precautionary actions to reduce the risk of infection within their community.

Conclusion

Participatory approach highlighted the importance of engaging with and integrating to local culture and values to overcome challenges such as gender imbalance and distrust. Findings of this study are relevant to others working in diverse cultural settings in similar crises events regardless of particular cultural variations.
Metformin mitigates SASP secretion and LPS-triggered hyper-inflammation in Doxorubicin-induced senescent endothelial cells
Authors: Abdelgawad Ibrahim Y. , Agostinucci Kevin , Sadaf Bushra , Grant Marianne K. O. , Zordoky Beshay N.
Published: April 2023

Introduction: Doxorubicin (DOX), a chemotherapeutic drug, induces senescence and increases the secretion of senescence-associated secretory phenotype (SASP) in endothelial cells (ECs), which contributes to DOX-induced inflammaging. Metformin, an anti-diabetic drug, demonstrates senomorphic effects on different models of senescence. However, the effects of metformin on DOX-induced endothelial senescence have not been reported before. Senescent ECs exhibit a hyper-inflammatory response to lipopolysachharide (LPS). Therefore, in our current work, we identified the effects of metformin on DOX-induced endothelial senescence and LPS-induced hyper-inflammation in senescent ECs.

Methods: ECs were treated with DOX ± metformin for 24 h followed by 72 h incubation without DOX to establish senescence. Effects of metformin on senescence markers expression, SA-β-gal activity, and SASP secretion were assessed. To delineate the molecular mechanisms, the effects of metformin on major signaling pathways were determined. The effect of LPS ± metformin was determined by stimulating both senescent and non-senescent ECs with LPS for an additional 24 h.

Results: Metformin corrected DOX-induced upregulation of senescence markers and decreased the secretion of SASP factors and adhesion molecules. These effects were associated with a significant inhibition of the JNK and NF-κB pathway. A significant hyper-inflammatory response to LPS was observed in DOX-induced senescent ECs compared to non-senescent ECs. Metformin blunted LPS-induced upregulation of pro-inflammatory SASP factors.

Conclusion: Our study demonstrates that metformin mitigates DOX-induced endothelial senescence phenotype and ameliorates the hyper-inflammatory response to LPS. These findings suggest that metformin may protect against DOX-induced vascular aging and endothelial dysfunction and ameliorate infection-induced hyper-inflammation in DOX-treated cancer survivors.
Social and executive functioning in individuals with autism spectrum disorder without intellectual disability: The case–control study protocol of the CNeSA study
Authors: Donno Federica , Balia Carla , Boi Jessica , Manchia Mirko , Zuddas Alessandro , Carucci Sara
Published: April 2023

Several studies suggest that children and adolescents with autism spectrum disorder (ASD) often present deficits in executive functions (EFs). The research on cold EF shows a high heterogeneity across different cohorts of patients as well as different study designs, while studies investigating hot EF and their relationship with different ASD phenotypes are still limited and related only to specific domains, although this concept could contribute to clarify the phenotypical variability by explaining the difficulties encountered by individuals with ASD in daily life, where stimuli are often emotionally charged. With the aim to identify specific neuropsychological profiles in children and adolescents with ASD without intellectual disability, we designed a study protocol comparing a clinical sample of individuals with ASD to aged-matched (10–17 years) typically developing controls (TDC) on a neuropsychological test battery investigating both “cold” and “hot” EF with the purpose of further investigating their relationships with ASD symptoms. Autonomic measures including heart rate, heart rate variability, skin conductance, and salivary cortisol were also recorded before/during/after the neuropsychological testing session. This paper describes the case–control study protocol named “Caratterizzazione NEuropsicologica del disturbo dello Spettro Autistico, senza Disabilità Intellettiva, CNeSA study,” its rationale, the specific outcome measures, and their implications for the clinical management of individuals with ASD and a precision medicine approach.
“It is something that gives us hope”: Lived experience among parents to children with cerebral palsy who are non-ambulant of the phenomenon physical activity, with or without the use of a novel dynamic standing device
Authors: Lauruschkus Katarina , Holmberg Robert , Tornberg Åsa B.
Published: April 2023

Introduction

Regular physical activity confers health benefits for all. Parents commonly want their children to be physically active, and want to be physically active themselves, but children with cerebral palsy (CP) who are non-ambulant face challenges, and they need support to be physically active. Dynamic standing in the novel motorized assistive device Innowalk has positive effects in children who are non-ambulant—it gives them a chance to be physically active. The aim of this study was to explore the lived experience of physical activity of parents themselves and for their children with cerebral palsy who are non-ambulant.

Methods

A descriptive inductive design with a hermeneutic phenomenological approach was used for the analysis of interviews with 11 parents of children with CP who are non-ambulant who participated in a study of exercise effects of dynamic standing.

Results

The parents experienced physical activity for their children as being important but difficult, especially for their child, as described in Theme 1: “Being aware of health benefits while struggling with family time.” The children were perceived as being dependent on other people, the environment, and equipment for participating in physical activity, referring to Theme 2: “Being dependent.” The opportunity for their children to become physically active on a regular basis through an assistive device gave the parents hope for a better life, which formed Theme 3: “Getting hope in a challenging life situation.”

Conclusion

Physical activity for children with CP who are non-ambulant is possible through an elaborate network of social relations and environmental conditions. Limiting the degree of dependence and containing the negative consequences of high a degree of dependence are vital in the support of physical activity. Relations, support, and assistive devices that strengthen empowerment and autonomy should be prioritized, and if this works, the experience of physical activity can be positive, giving families hope.
Recent trends in robot learning and evolution for swarm robotics
Authors: Kuckling Jonas
Published: April 2023

Swarm robotics is a promising approach to control large groups of robots. However, designing the individual behavior of the robots so that a desired collective behavior emerges is still a major challenge. In recent years, many advances in the automatic design of control software for robot swarms have been made, thus making automatic design a promising tool to address this challenge. In this article, I highlight and discuss recent advances and trends in offline robot evolution, embodied evolution, and offline robot learning for swarm robotics. For each approach, I describe recent design methods of interest, and commonly encountered challenges. In addition to the review, I provide a perspective on recent trends and discuss how they might influence future research to help address the remaining challenges of designing robot swarms.
Soil quality and crop productivity under 34 years old long-term rainfed rice based cropping system in an Inceptisol of sub-tropical India
Authors: Biswas Sunanda , Singh Priya , Rahaman Riaj , Patil Kalyani V. , De Nirmal
Published: April 2023

Introduction

Soil quality deterioration with the introduction of modern agriculture is a major threat to agricultural sustainability and food security and the problem is more aggravated specially under rainfed agriculture. Asessment of soil quality is a tortuous task as it can not be measured directly. The objective of the present investigation was to evaluate the effect of long-term fertilization and manuring on soil quality and identify the most sensitive indicators of assessing soil quality under rainfed rice based system.

Methods

Soil samples were collected from selected six treatments viz. control, 100%NPK, 50%NPK, 50%FYM, 100%FYM and 50%NPK+FYM of 34 years old long-term fertilizer experiment with rainfed rice-lentil cropping system situated at BHU Varanasi, India.

Results and discussion

Result revealed that continuous organic manure application along with inorganic fertilizer increased soil organic carbon by 54.1% over control treatment.Principal component analysis (PCA) was done to screen out key indicators and mean weight diameter, available Fe, available N, potentially mineralizable N, available Zn, FDA hydrolase activity and Clay were selected as key indicators of soil quality. The highest soil quality index (SQI) of 0.95 was found in 50% NPK+FYM treatment. Regression analysis showed better agreement of equivalent rice yield and SQI (0.87). Therefore, the balanced fertilization with organic and inorganic fertilizers is important for sustainability of the rainfed rice-lentil cropping system and this practice may be recommended for rainfed rice based system of Indian Inceptisol.
The need and development for a value-added toolkit—A case study with Montana specialty fruit growers
Authors: Garg Sumedha , Jha Gaurav , Kim Sun-Hwa , Miller Zachariah , Kuo Wan-Yuan
Published: April 2023

Introduction

Cold-hardy small fruits and berries have the potential for specialty fruit growers in the Intermountain West, where the climate is not suited for conventional fruit crops. In the last seven years, approximately 50 varieties of cold-hardy, bush fruit types have been researched in western Montana.

Method

Hence with the increased small fruit and berry production, and interest of specialty fruit growers in value-added product development, this paper utilized participatory action research (PAR) to develop a value-added product development toolkit with specialty fruit growers and used an integrated logic model to discuss creating and implementing the toolkit. Firstly, we used an online survey to identify the needs and challenges of specialty fruit growers. Next, a value-added toolkit is drafted using the principles of design thinking and involving a student-grower partnership. Thirdly, the specialty fruit grower's interest in and feedback on the drafted toolkit is evaluated using focus group discussions and individuals interviews, and the results are used to revise the toolkit. Lastly, the short, medium and long-term outcomes for this toolkit are discussed using the logic model.

Results

From the survey, 61% of specialty fruit growers indicated an interest in value-added opportunities. Yet, focus group discussions and individual interviews found the biggest barriers to value-added product development are cost, resources, and environment. This indicated a co-created toolkit will be a beneficial solution. During focus group and individual interviews, the growers suggested including the toolkit as part of coursework in semester-long classes. This will address issues of continuity and funding.

Discussion

Overall, this study deployed PAR methods to propose with Montana specialty fruit growers a solution to their increasing needs in value-added pursuits, implying short-term economic benefits but also long-term socio-ecological benefits. The participatory model of creating value-added resources presented by this paper can benefit other small-scale specialty crop growers in underserved regions.
Expression significance of Emi1, UBCH10 and CyclinB1 in esophageal squamous cell carcinoma
Authors: Li Hui , Yang Chenbo , Chen Kuisheng , Sun Miaomiao
Published: April 2023

Despite significant advances in the diagnosis and treatment of esophageal squamous cell carcinoma (ESCC), esophageal cancer is still a heavy social and medical burden due to its high incidence. Uncontrolled division and proliferation is one of the characteristics of tumor cells, which will promote rapid tumor growth and metastasis. Early mitotic inhibitor 1 (Emi1), ubiquitin-conjugating enzyme 10 (UBCH10) and CyclinB1 are important proteins involved in the regulation of cell cycle. In this study, the expression of Emi1, UBCH10 and CyclinB1 in ESCC tissues and adjacent normal tissues will be analyzed by immunohistochemistry and in-situ hybridization techniques, and their relationship with tumor proliferation and apoptosis will be analyzed. The results showed that Emi1, UBCH10 and CyclinB1 genes and proteins were highly expressed in tumor tissues, which were correlated with tumor grade, lymph node metastasis and pathological stage, and positively correlated with tumor proliferation. Emi1, UBCH10 and CyclinB1 are also positively correlated. It is speculated that Emi1, UBCH10 and CyclinB1 genes synergically promote tumor proliferation and inhibit apoptosis, which may be potential diagnostic and therapeutic targets for ESCC.
A Novel Hybrid Recommender System for the Tourism Domain
Authors: Chalkiadakis Georgios , Ziogas Ioannis , Koutsmanis Michail , Streviniotis Errikos , Panagiotakis Costas , Papadakis Harris
Published: April 2023

In this paper, we develop a novel hybrid recommender system for the tourism domain, which combines (a) a Bayesian preferences elicitation component which operates by asking the user to rate generic images (corresponding to generic types of POIs) in order to build a user model and (b) a novel content-based (CB) recommendations component. The second component can in fact itself be considered a hybrid among two different CB algorithms, each exploiting one of two semantic similarity measures: a hierarchy-based and a non-hierarchy based one. The latter is the recently introduced Weighted Extended Jaccard Similarity (WEJS). We note that WEJS is employed for the first time within a recommender algorithm. We incorporate our algorithm within a real, already available at Google Play, tour-planning mobile application for short-term visitors of the popular touristic destination of Agios Nikolaos, Crete, Greece, and evaluate our approach via extensive simulations conducted on a real-world dataset constructed for the needs of the aforementioned mobile application. Our experiments verify that our algorithms result in effective personalized recommendations of touristic points of interest, while our final hybrid algorithm outperforms our exclusively content-based recommender algorithms in terms of recommendations accuracy. Specifically, when comparing the performance of several hybrid recommender system variants, we are able to come up with a “winner”: the most preferable variant of our hybrid recommender algorithm is one using a ⟨four elicitation slates, six shown images per slate⟩ pair as input to its Bayesian elicitation component. This variant combines increased precision performance with a lightweight preferences elicitation process.
Design and Coverage Path Planning of a Disinfection Robot
Authors: Xu Pengjie , Chen Xinyi , Tang Qirong
Published: April 2023

Eliminating pathogen exposure is an important approach to control outbreaks of epidemics such as COVID-19 (coronavirus disease 2019). To deal with pathogenic environments, using disinfection robots is a practicable choice. This research formulates a 3D (three-dimensional) spatial disinfection strategy for a disinfection robot. First, a disinfection robot is designed with an extensible control framework for the integration of additional functions. The robot has eight degrees of freedom that can handle disinfection tasks in complex 3D environments where normal disinfection robots lack the capability to ensure complete disinfection. An ingenious clamping mechanism is designed to increase flexibility and adaptability. Secondly, a new coverage path planning algorithm targeted at the spraying area is used. This algorithm aims to achieve an optimal path via the rotating calipers algorithm after transformation between a 2D (two-dimensional) array and 3D space. Finally, the performance of the designed robot is tested through a series of simulations and experiments in various spaces that humans usually live in. The results demonstrate that the robot can effectively perform disinfection tasks both in computer simulation and in reality.
Development of a Global Model for the Analysis of Plasma in an Atmosphere-Breathing Cathode-Less Thruster
Authors: Dalle Fabbriche Simone , Souhair Nabil , Magarotto Mirko , Andriulli Raoul , Corti Enrico , Ponti Fabrizio
Published: April 2023

This study investigates the preliminary propulsive performances of a cathode-less plasma thruster with air as its propellant. The analysis is carried out through a global model and simulates a thruster over a power range of 0 to 50 W. The developed code considers a set of 177 chemical reactions involving 8 different species and includes empirical equations to account for electronegative effects. The analysis presents the steady-state values of species densities at 10 W, 30 W, and 50 W to gain insights into the key characteristics of plasma dynamics. Moreover, the study estimates the thrust and specific impulse and compares the results to data from models that employ xenon and iodine, aiming to understand the performances of air in low-power thrusters. Lastly, the study examines the effects of varying air inflow concentration on the chemistry, analyzing three different orbit altitudes (i.e., 200, 300, and 400 km).
Explaining the Lack of Mesh Convergence of Inviscid Adjoint Solutions near Solid Walls for Subcritical Flows
Authors: Lozano Carlos , Ponsin Jorge
Published: April 2023

Numerical solutions to the adjoint Euler equations have been found to diverge with mesh refinement near walls for a variety of flow conditions and geometry configurations. The issue is reviewed, and an explanation is provided by comparing a numerical incompressible adjoint solution with an analytic adjoint solution, showing that the anomaly observed in numerical computations is caused by a divergence of the analytic solution at the wall. The singularity causing this divergence is of the same type as the well-known singularity along the incoming stagnation streamline, and both originate at the adjoint singularity at the trailing edge. The argument is extended to cover the fully compressible case, in subcritical flow conditions, by presenting an analytic solution that follows the same structure as the incompressible one.
The Effect of Glutamine as Feed Additive on Selected Parameters of the Nonspecific Immune Response in Pigs
Authors: Jarosz Łukasz S. , Tomaszewska Ewa , Marek Agnieszka , Hejdysz Marcin , Burmańczuk Artur , Ciszewski Artur , Nowaczewski Sebastian , Grądzki Zbigniew , Batorski Maciej , Świątkiewicz Małgorzata , Rysiak Anna
Published: April 2023

The use of feed additives containing glutamine can influence the growth and development of piglets during the weaning period. The aim of this study was to determine the effect of feed supplementation with 0.5% L-glutamine on selected parameters of the nonspecific immune response of pigs. The research was carried out on 60 pigs (Polish Large White × Polish Landrace), from 28 days of age to slaughter. The obtained results showed an increased percentage of phagocytic cells (monocytes and granulocytes) and oxygen blast cells in pigs between 28 and 70 days of age, proving that non-specific immune mechanisms were stimulated, which contributed to the improvement of the processes of antigen elimination from the body. The increase in the percentage of cells expressing SWC3, CD11b/CD18+, CD14+ and CD14+CD16+ molecules on granulocytes and monocytes during this period resulted in the enhancement of the host defense mechanisms by stimulating phagocytosis and enhancing the mechanisms of a non-specific immune response. The high concentration of TNF-α and IL-1β as well as Il-10 in the experimental group indicates the cellular phenotype of the Th1-type response, and the maintenance of the immune balance between the pro-inflammatory and anti-inflammatory responses and ensuring the homeostasis of the organism.
Risk Assessment of Sulfonylurea Herbicides Based on a Complex Bioindicator
Authors: Borozan Aurica , Bordean Despina-Maria , Boldura Oana , Popescu Sorina , Caraba Marioara , Moldovan Camelia
Published: April 2023

The increasing use of herbicides in recent years for improved crop yields requires a risk assessment. To assess their impact on soil, the use of an indicator named the synthetic biological indicator (ISB%) is proposed, which includes a range of biotic and enzymatic parameters derived from previous experiments. Three sulfonylurea herbicides were evaluated, named chlorsulfuron, amidosulfuron, and tifensulfuron. The biotic and enzymatic parameters were monitored using different herbicide doses in field and laboratory experiments. Calculating this indicator for all experimental variants in the field and laboratory showed that the impact of the analyzed herbicides was insignificant, but there were statistically significant differences between the experimental conditions. The registration of an herbicide based on the legislation of different countries requires several toxicity tests of the active substance’s effects against soil microorganisms and some of the soil functions performed by microorganisms, parameters which are also included in the synthetic biological indicator (ISB). This indicator has the capacity to provide important information for sustainable soil management, including a minimum set of parameters, which can provide global information regarding the environment, showing changes in multiple areas of interest, including parameters that can be applied at minimal cost worldwide. In conclusion, we can say that the use of the indicator highlights all the changes caused by various soil chemical treatments because it follows the variation in a large number of parameters, unlike other indicators that follow only one, providing useful information for sustainable farming practices.
Mapping of QTLs for Brown Rice Traits Based on Chromosome Segment Substitution Line in Rice (Oryza sativa L.)
Authors: Leng Yujia , Hong Lianmin , Tao Tao , Guo Qianqian , Yang Qingqing , Zhang Mingqiu , Ren Xinzhe , Jin Sukui , Cai Xiuling , Gao Jiping
Published: April 2023

Brown rice traits are critical to both grain yield and quality. In the present study, the chromosome segment substitution lines (CSSLs) population derived from a cross between japonica Koshihikari and indica Nona Bokra was used to analyze the brown rice length (BRL), brown rice width (BRW), length–width ratio of brown rice (BLWR), brown rice thickness (BRT), brown rice perimeter (BRP), brown rice area (BRA), thousand-grain weight of brown rice (BRGW), brown rice ratio (BRR), taste value of brown rice (BTV), and water content of brown rice (BWC). Correlations analysis showed that most of the brown rice traits had significant correlations with each other, except for BRR, BTV, and BWC. A total of sixty-one QTLs for these traits were identified under three environments, which mapped to chromosomes 1, 2, 3, 5, 6, 7, 8, 10, 11, and 12, with the LOD ranging from 2.52 to 16.68 and accounting for 2.60 to 25.38% of the total phenotypic variations. Moreover, thirty pairs of epistatic interactions for BRL, BRW, BLWR, BRP, and BRA were estimated and distributed on all chromosomes except 10. These findings will provide a further understanding of the genetic basis of brown rice traits and facilitate the genetic improvement of rice yield and quality through breeding.
OsHSP 17.9, a Small Heat Shock Protein, Confers Improved Productivity and Tolerance to High Temperature and Salinity in a Natural Paddy Field in Transgenic Rice Plants
Authors: Do Jeong-Mi , Kim Hee-Jin , Shin Sun-Young , Park Seong-Im , Kim Jin-Ju , Yoon Ho-Sung
Published: April 2023

Various abiotic stress factors, such as high temperatures and salinity, have a significant impact on the development and growth of crop plants and ultimately impact crop yield. Previous studies have reported that overexpression of heat-shock-protein (HSP) genes in transgenic plants can enhance stress tolerance under controlled conditions in laboratories and greenhouses. Despite the significance of multiple environmental stressors on plants in natural paddy fields, there is still a lack of research regarding the contribution of HSP genes to stress tolerance and crop yield. In this study, we cloned and characterized the function of OsHSP 17.9, an HSP gene from Oryza sativa, in rice plants grown under diverse conditions. Our results showed that overexpressing OsHSP 17.9 in rice plants enhanced the activity of antioxidant enzymes under high-temperature and salinity stresses. Moreover, transgenic rice plants overexpressing OsHSP 17.9 exhibited significantly improved adaptability after transplantation from greenhouses to natural paddy fields. In particular, OsHSP 17.9-overexpressing transgenic rice plants established improved agronomic traits and increased grain yields even under unfavorable natural-paddy-field conditions. These results suggest that OsHSP 17.9 transgenic plants can be a promising strategy for cultivating crops in adverse environmental conditions.
Research on Tractor Optimal Obstacle Avoidance Path Planning for Improving Navigation Accuracy and Avoiding Land Waste
Authors: Chen Hongtao , Xie Hui , Sun Liming , Shang Tansu
Published: April 2023

Obstacle avoidance operations of tractors can cause parts of land to be unavailable for planting crops, which represents a reduction in land utilization. However, land utilization is significant to the increase in agricultural productivity. Traditional obstacle avoidance path planning methods mostly focus on automatic tractor navigation with small errors, ignoring the decrease in land utilization due to obstacle avoidance operations. To address the problem, this paper proposed an obstacle avoidance path planning method based on the Genetic Algorithm (GA) and Bezier curve. In this paper, a third-order Bezier curve was used to plot the obstacle avoidance path, and the range of control points for the third-order Bezier curve was determined according to the global path and the location of the obstacle. To target the navigation error and land utilization problems, GA was used to search for the optimal point from the selection range of the control point under multiple constraints for automatic tractor navigation such as the obstacle collision avoidance, the minimum turning radius, and the maximum turning angle. Finally, the optimal obstacle avoidance path was determined based on the selected control points to minimize the navigation error and maximize land utilization. The algorithm proposed in this paper was compared with existing methods and the results showed that it has generally favorable performance on obstacle avoidance path planning.
Nano-Hydroxyapatite and ZnO-NPs Mitigate Pb Stress in Maize
Authors: Alhammad Bushra Ahmed , Ahmad Awais , Seleiman Mahmoud F.
Published: April 2023

Heavy metals (HMs) stress, particularly lead (Pb) stress, is one of the most hazardous environmental stresses that can negatively affect plants’ growth, yield, and quality. Therefore, the effects of zinc oxide nanoparticles (ZnO-NPs; 50 mg L−1), nano-hydroxyapatite (HP-NPs; 50 mg kg−1), and their combination on growth, physiological, and yield traits of maize grown in soil contaminated with Pb (i.e., 100 mg kg−1) were investigated. The results showed that Pb stress significantly reduced plant leaf area by 50.9% at 40 days after sowing (DAS), 55.5% at 70 DAS, and 54.2% at 100 DAS in comparison to the unstressed plants (control). However, the combined application of ZnO-NPs (50 mg L−1) + HP-NPs (50 mg kg−1) reduced the adverse effects of Pb on plant growth in terms of increasing leaf area by 117.6% in plants grown in Pb-contaminated soil (100 mg kg−1). Similarly, the combined application of ZnO-NPs + HP-NPs resulted in increments in the total chlorophyll content by 47.1%, photosynthesis rate by 255.1%, and stomatal conductance by 380% in comparison to that obtained from maize stressed with Pb. On the other hand, antioxidants such as sodium dismutase (SOD; 87.1%), peroxidase (POX; 90.8%), and catalase (CAT; 146%), and proline content (116%) were significantly increased as a result of Pb stress compared to unstressed plants. Moreover, N, P, K, and Zn contents in the whole plant grown under Pb stress were decreased by 38.7%, 69.9%, 46.8%, and 82.1%, respectively, compared to those obtained from the control. Whereas the combined treatment of ZnO-NPs (50 mg L−1) + HP-NPs (50 mg kg−1) resulted in increased uptake of plant nutrients and, consequently, the highest values of ear weight, grain yield, and harvest index were obtained. Furthermore, the combined application of HP-NPs + ZnO-NPs in contaminated soil reduced Pb uptake in plant biomass by 77.6% and grains by 90.21% in plants exposed to Pb stress. In conclusion, the combined application of ZnO-NPs and HP-NPs significantly improved growth, physiological traits, antioxidants, and yield as well as elemental uptake of maize grown under Pb stress.
Molecular Basis of Resistance to Bensulfuron-Methyl in a Smallflower Umbrella Sedge (Cyperus difformis L.) Population from China
Authors: Yin Shanshan , Hu Wei , Chai Yin , Jiang Minghao , Zhang Jingxu , Cao Haiqun , Zhao Ning , Liao Min
Published: April 2023

Smallflower umbrella sedge (Cyperus difformis L.) is an invasive weed, and infestations of C. difformis are increasing in rice (Oryza sativa L.) fields in China. Bensulfuron-methyl is a widely used sulfonylurea herbicide that inhibits the acetolactate synthase (ALS) enzyme and has been used in recent years for effectively controlling annual weeds in the Cyperaceae family. In this study, a suspected resistant population of C. difformis (BBHY1) was collected from a rice field in Huaiyuan County, Anhui Province, China, that survived treatment with bensulfuron-methyl at the field-recommended rate (FRR). Single-dose tests and whole-plant bioassays confirmed that the BBHY1 population was resistant to bensulfuron-methyl and had evolved a high level of resistance, with a resistance index (RI) of 12.87. Sequencing of the ALS gene revealed a CCT to CAT point mutation at codon 197, which caused a P-to-H substitution in the resistant plants. Analysis of the relative expression of ALS revealed no significant differences between the resistant and susceptible populations. Inhibiting the activity of cytochrome P450s (P450s) or glutathione S-transferases (GSTs) had no significant effect on bensulfuron-methyl resistance. The BBHY1 population exhibited cross-resistance to pyrazosulfuron-ethyl, penoxsulam, and bispyribac-sodium, with RIs ranging from 5.48 to 20.63, but remained susceptible to MCPA sodium, florpyrauxifen-benzyl, and bentazon, with RIs of <1.00. These herbicides could be potentially used as alternatives for controlling resistant populations and managing herbicide resistance in other aggressive weeds in rice fields.
GWAS Characterization of the Genetic Regions Associated with Nine Important Agronomic Traits in Tomato (Solanum lycopersicum L.)
Authors: Liu Zengbing , Jiang Jingbin , Li Jingfu
Published: April 2023

Understanding the genetic background of elite cultivated tomato germplasm resources in crossbreeding and revealing the genetic basis of complex traits are vital for better-targeted germplasm expansion and the creation of strong hybrids. Here, we obtained approximately 21 million single-nucleotide polymorphisms (SNPs) based on the sequencing of 212 cultivated tomato accessions and the population structure of which was revealed. More importantly, we found that target genes distributed on chromosomes 1, 5, 9, and 11 may be actively selected in breeding. In particular, the significant signals related to soluble sugar content (chr1_94170222, chr1_96273188, chr9_4167512, and chr11_55592768), fruit firmness (chr5_4384919 and chr5_5111452) and gray leaf spot resistance (chr11_8935252 and chr11_9707993) were also detected on the corresponding chromosomes, respectively. Overall, we reported 28 significant association signals for nine agronomic traits based on a mixed linear model (MLM), including 114 genes. Among these signals, 21 contained potential novel genes for six fruit traits. These novel candidate genes located in genomic regions without previously known loci or on different chromosomes explained approximately 16% of the phenotypic variance on average in cultivated tomatoes. These findings could accelerate the identification and validation of novel and known candidate genes and QTLs, improving the understanding of the genetic structure of complex quantitative traits. These results also provide a basis for tomato breeding and improvement.