bluuebunny/crossref_metadata_2025 huggingface dataset:
A microCT-based atlas of the central nervous system and midgut in sea spiders (Pycnogonida) sheds first light on evolutionary trends at the family level
Authors: Frankowski Karina , Miyazaki Katsumi , Brenneis Georg
Published: March 2022

Background

Pycnogonida (sea spiders) is the sister group of all other extant chelicerates (spiders, scorpions and relatives) and thus represents an important taxon to inform early chelicerate evolution. Notably, phylogenetic analyses have challenged traditional hypotheses on the relationships of the major pycnogonid lineages (families), indicating external morphological traits previously used to deduce inter-familial affinities to be highly homoplastic. This erodes some of the support for phylogenetic information content in external morphology and calls for the study of additional data classes to test and underpin in-group relationships advocated in molecular analyses. In this regard, pycnogonid internal anatomy remains largely unexplored and taxon coverage in the studies available is limited.

Results

Based on micro-computed X-ray tomography and 3D reconstruction, we created a comprehensive atlas of in-situ representations of the central nervous system and midgut layout in all pycnogonid families. Beyond that, immunolabeling for tubulin and synapsin was used to reveal selected details of ganglionic architecture. The ventral nerve cord consistently features an array of separate ganglia, but some lineages exhibit extended composite ganglia, due to neuromere fusion. Further, inter-ganglionic distances and ganglion positions relative to segment borders vary, with an anterior shift in several families. Intersegmental nerves target longitudinal muscles and are lacking if the latter are reduced. Across families, the midgut displays linear leg diverticula. In Pycnogonidae, however, complex multi-branching diverticula occur, which may be evolutionarily correlated with a reduction of the heart.

Conclusions

Several gross neuroanatomical features are linked to external morphology, including intersegmental nerve reduction in concert with trunk segment fusion, or antero-posterior ganglion shifts in partial correlation to trunk elongation/compaction. Mapping on a recent phylogenomic phylogeny shows disjunct distributions of these traits. Other characters show no such dependency and help to underpin closer affinities in sub-branches of the pycnogonid tree, as exemplified by the tripartite subesophageal ganglion of Pycnogonidae and Rhynchothoracidae. Building on this gross anatomical atlas, future studies should now aim to leverage the full potential of neuroanatomy for phylogenetic interrogation by deciphering pycnogonid nervous system architecture in more detail, given that pioneering work on neuron subsets revealed complex character sets with unequivocal homologies across some families.
Functional analysis of a viral promoter from a strawberry vein banding virus isolate from China
Authors: Jiang Lei , Chen Jing , Yang You-zhi , Li Rui , Li Shuang , Wang Zhan-qi , Jiang Tong
Published: March 2022

Background

Promoters are important factors affecting gene expression in cells. The driven activities of viral promoters were generally assessed to screen available promoters for transgenic and research and biotech industries. In this study, we cloned a full-length promoter from a Chinese isolate of strawberry vein banding virus (SVBV) and produced several deletion mutants for evaluation of applications in production of reporter proteins in stable transgenic plants.

Methods

The full-length promoter of SVBV (SP1) and its three deletion mutants (SP2, SP3, and SP4) were amplified using polymerase chain reaction. The effects of SVBV SP1, SP2, SP3, and SP4 on gene expression were evaluated using β-glucuronidase (GUS) and green fluorescent protein (GFP) reporter genes.

Results

Transient expression assays showed that the SVBV SP1 promoter and its three deletion mutants all expressed the reporter genes, albeit at very different levels. Interestingly, transcriptional activity driven by the SP1 promoter was much higher than that of the cauliflower mosaic virus (CaMV) 35S promoter. After stable transformation of the GUS gene into Nicotiana tabacum plants, SVBV SP1-driven transgene expression was approximately 2.6-fold higher than CaMV 35S promoter-driven transgene expression. In addition, GUS gene expression levels were enhanced by co-inoculation of the plants with the SP1 promoter-driven vector carrying the GUS gene and the vector expressing SVBV open reading frame (ORF) V or ORF VI.

Conclusions

The SVBV SP1 promoter from the Chinese isolate evaluated in this study could successfully drive transient and stable expression in plants, it was a stronger promoter than the CaMV 35S and FLt-US promoters and may be more useful for the production of stable transgenic plants.
Vascular endothelial growth factor-A level in human breast milk after intravitreal injection of ranibizumab: a case report
Authors: Huang Ying , Zhou Rong , Sun Zuhua , Zheng Yihan , Lin Bing
Published: March 2022

Background

Ranibizumab is one of intravitreal anti-vascular endothelial growth factor agents. It is applied in the treatments of choroidal neovascularization, age-related macular degeneration, diabetic macular edema, and macular edema secondary to retinal vein occlusion. Preliminary evidence suggests that intravitreal ranibizumab may enter the plasma and human breast milk in very low-level concentration. As a precaution, breastfeeding is not recommended during the treatment of intravitreal injection of ranibizumab. There are limited data regarding the change of anti-vascular endothelial growth factor concentration in human breast milk after intravitreal injection of ranibizumab, especially in the first 24 h after injection. The purpose of this report is to analyse the concentration change of vascular endothelial growth factor-A in human breast milk with time, in the short term after intravitreal injection of ranibizumab.

Case presentation

In June 2018, a 30-year-old patient breastfeeding a six-month-old baby was diagnosed with choroidal neovascularization of left eye in Eye Hospital of Wenzhou Medical University. She received four administrations of 0.5 mg intravitreal injection of ranibizumab of the left eye, and breast milk was collected just before the injection, and 1–3, 6, 12, 24, 48, and 72 h after intravitreal injection, and assessed for vascular endothelial growth factor-A concentration. The change in vascular endothelial growth factor-A concentration in human breast milk showed the same trend after each injection, decreasing significantly within 6–12 h (about 20–30% lower), and increasing to pre-injection level by 24 h after injection.

Conclusions

The concentration of vascular endothelial growth factor-A in human breast milk of a mother who continues lactating dropped initially and rose to pre-injection level about 24 h after intravitreal injection of ranibizumab. The data may offer more information to evaluate the impact of anti-vascular endothelial growth factor agent intravitreal injection of lactating mothers and their breastfed infants.
Traumatic events, daily stressors and posttraumatic stress in unaccompanied young refugees during their flight: a longitudinal cross-country study
Authors: Pfeiffer Elisa , Behrendt Malte , Adeyinka Sarah , Devlieger Ines , Rota Marina , Uzureau Océane , Verhaeghe Floor , Lietaert Ine , Derluyn Ilse
Published: March 2022

Background

Unaccompanied young refugees constitute an especially vulnerable population, reporting high rates of trauma and mental health problems. There is a significant gap in the literature on trauma and posttraumatic stress symptoms (PTSS) in unaccompanied young refugees who are still on the move and live in precarious circumstances such as refugee camps. This study therefore aimed to contribute to this gap by investigating pre- and peri-migration (potentially) traumatic experiences of unaccompanied young refugees; longitudinal trajectories of trauma, daily stressors and PTSS; and the impact of gender, trauma, and daily stressors on PTSS over time.

Methods

This longitudinal, mixed-method, and multi-country study was conducted in various settings (e.g. refugee camps, reception centers) across nine European countries. A heterogeneous sample of N = 187 unaccompanied young refugees (78.4% male) from 29 different countries was assessed via interviews at 3 time-points during a period of 27 months. Data was analyzed via growth curve modelling.

Results

Prevalence rates of (potentially) traumatic experiences ranged from 29.5 to 91.9%. Peri-migration traumatization remained stable over time (b = − 0.02; p = 0.371), but the number of reported daily stressors (b = − 0.24; p = 0.001) and PTSS scores significantly decreased over time (b = − 0.98; p = 0.004). Females reported higher PTSS compared with males at baseline (p = 0.002), but gender did not influence the longitudinal trajectory of PTSS. The pre-migration trauma load and daily stressors at baseline did not have a significant effect on PTSS at baseline or on the longitudinal trajectory.

Conclusions

This is the first study to document not only the high numbers of traumatic events for unaccompanied young refugees pre- and peri- migration, but also the continued traumatization during flight, as well as high rates of daily stressors and PTSS. Humanitarian and political assistance is urgently needed to curb the often life-threatening conditions unaccompanied young refugees face during migration.
CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling
Authors: Chen Dongni , Zhou Hongli , Cai Zhuochen , Cai Kaican , Liu Ji , Wang Weidong , Miao Huikai , Li Hongmu , Li Rongzhen , Li Xiaodong , Chen Youfang , Wang Hui-Yun , Wen Zhesheng
Published: April 2022

Background

Circular RNA (circRNA) has been recently identified as a critical regulator during carcinogenesis. However, the biological function and potential underlying mechanisms of circRNAs in lung cancer remain to be further elucidated.

Methods

Here, we first evaluated the differentially expressed circRNAs between tumor and the matched adjacent nontumor tissues (3 pairs) of lung cancer patients via circRNA microarray. The expression of top five dysregulated circRNAs were tested in lung cancer cell lines and the circSCAP with concordant alteration in microarray data and cell lines was selected for further investigation. Then we validated the expression level of circSCAP in tumor and corresponding adjacent tissues (161 pairs) from a lung cancer cohort by RT-PCR analysis followed by correlation and prognosis analysis between circSCAP and clinical characteristics. Non-small cell lung cancer (NSCLC) accounts for the majority of lung cancer diagnosis (about 80% in the cohort used in this study). Therefore, we focused the role of circSCAP in NSCLC in the present study. In vitro and in vivo assays were performed to study the biological function of circSCAP in NSCLC. Biotin-labeled RNA pulldown and RNA immunoprecipitation (RIP) assays were carried out to identify the proteins directly interacting with circSCAP. The molecular mechanism of circSCAP-driven tumor suppression was demonstrated by immunoblotting, immunoprecipitation and luciferase reporter assays. In vitro and in vivo rescue experiments were conducted to verify the role of the circSCAP/SF3A3/p53 signaling axis in NSCLC.

Results

We screened the expression profiles of human circRNAs in lung cancer tissues and found that hsa_circ_0065214 (termed as circSCAP) was significantly decreased. Kaplan–Meier analysis showed that patients with low level of circSCAP had a significantly poor prognosis. Gain- and loss-of-function experiments suggested that circSCAP played an important role in NSCLC cell proliferation, cell migration and apoptosis. Mechanistically, circSCAP directly binds to the SF3A3 protein, facilitating the reduction of SF3A3 by promoting its ubiquitin–proteasome-mediated degradation, which enhances the expression of MDM4-S to finally activate its downstream p53 signaling.

Conclusion

These findings illustrate a novel circSCAP/SF3A3/p53 signaling axis involved in suppressing the malignance of NSCLC and provide a promising target for NSCLC prognosis prediction and treatment.
Pain and sedation management and monitoring in pediatric intensive care units across Europe: an ESPNIC survey
Authors: Daverio Marco , von Borell Florian , Ramelet Anne-Sylvie , Sperotto Francesca , Pokorna Paula , Brenner Sebastian , Mondardini Maria Cristina , Tibboel Dick , Amigoni Angela , Ista Erwin ,   the Analgosedation CONSORTIUM on behalf of the Pharmacology Section and the Nurse Science Section of the European Society of Paediatric and Neonatal Intensive Care, Kola Ermira , Vittinghoff Maria , Duval Elim , Polić Branka , Valla Frédéric , Neunhoeffer Felix , Konstantinos Tziouvas , Györgyi Zoltán , Tan Mong Hoi , Hasani Antigona , Poluzioroviene Edita , Balmaks Reinis , Afanetti Mickael , Bentsen Gunnar , Bartkowska-Sniatkowska Alicja , Camilo Cristina , Simic Dusica , López-Fernández Yolanda M. , Mattsson Janet , Özen Hasan , Dmytriiev Dmytro , Manning Joseph C. , Tekgüç Hakan
Published: March 2022

Background

Management and monitoring of pain and sedation to reduce discomfort as well as side effects, such as over- and under-sedation, withdrawal syndrome and delirium, is an integral part of pediatric intensive care practice. However, the current state of management and monitoring of analgosedation across European pediatric intensive care units (PICUs) remains unknown. The aim of this survey was to describe current practices across European PICUs regarding the management and monitoring of pain and sedation.

Methods

An online survey was distributed among 357 European PICUs assessing demographic features, drug choices and dosing, as well as usage of instruments for monitoring pain and sedation. We also compared low- and high-volume PICUs practices. Responses were collected from January to April 2021.

Results

A total of 215 (60% response rate) PICUs from 27 European countries responded. Seventy-one percent of PICUs stated to use protocols for analgosedation management, more frequently in low-volume PICUs (77% vs 63%, p = 0.028). First-choice drug combination was an opioid with a benzodiazepine, namely fentanyl (51%) and midazolam (71%) being the preferred drugs. The starting doses differed between PICUs from 0.1 to 5 mcg/kg/h for fentanyl, and 0.01 to 0.5 mg/kg/h for midazolam. Daily assessment and documentation for pain (81%) and sedation (87%) was reported by most of the PICUs, using the preferred validated FLACC scale (54%) and the COMFORT Behavioural scale (48%), respectively. Both analgesia and sedation were mainly monitored by nurses (92% and 84%, respectively). Eighty-six percent of the responding PICUs stated to use neuromuscular blocking agents in some scenarios. Monitoring of paralysed patients was preferably done by observation of vital signs with electronic devices support.

Conclusions

This survey provides an overview of current analgosedation practices among European PICUs. Drugs of choice, dosing and assessment strategies were shown to differ widely. Further research and development of evidence-based guidelines for optimal drug dosing and analgosedation assessment are needed.
Prime-seq, efficient and powerful bulk RNA sequencing
Authors: Janjic Aleksandar , Wange Lucas E. , Bagnoli Johannes W. , Geuder Johanna , Nguyen Phong , Richter Daniel , Vieth Beate , Vick Binje , Jeremias Irmela , Ziegenhain Christoph , Hellmann Ines , Enard Wolfgang
Published: March 2022

Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.
The effect of extracorporeal shock wave therapy in acute traumatic spinal cord injury on motor and sensory function within 6 months post-injury: a study protocol for a two-arm three-stage adaptive, prospective, multi-center, randomized, blinded, placebo-controlled clinical trial
Authors: Leister Iris , Mittermayr Rainer , Mattiassich Georg , Aigner Ludwig , Haider Thomas , Machegger Lukas , Kindermann Harald , Grazer-Horacek Anja , Holfeld Johannes , Schaden Wolfgang
Published: April 2022

Background

The pathological mechanism in acute spinal cord injury (SCI) is dual sequential: the primary mechanical lesion and the secondary injury due to a cascade of biochemical and pathological changes initiated by the primary lesion. Therapeutic approaches have focused on modulating the mechanisms of secondary injury. Despite extensive efforts in the treatment of SCI, there is yet no causal, curative treatment approach available.

Extracorporeal shock wave therapy (ESWT) has been successfully implemented in clinical use. Biological responses to therapeutic shock waves include altered metabolic activity of various cell types due to direct and indirect mechanotransduction leading to improved migration, proliferation, chemotaxis, modulation of the inflammatory response, angiogenesis, and neovascularization, thus inducing rather a regeneration than repair.

The aim of this clinical study is to investigate the effect of ESWT in humans within the first 48 h after an acute traumatic SCI, with the objective to intervene in the secondary injury phase in order to reduce the extent of neuronal loss.

Methods

This two-arm three-stage adaptive, prospective, multi-center, randomized, blinded, placebo-controlled study has been initiated in July 2020, and a total of 82 patients with acute traumatic SCI will be recruited for the first stage in 15 participating hospitals as part of a two-armed three-stage adaptive trial design. The focused ESWT (energy flux density: 0.1–0.19 mJ/mm2, frequency: 2–5 Hz) is applied once at the level of the lesion, five segments above/below, and on the plantar surface of both feet within the first 48 h after trauma.

The degree of improvement in motor and sensory function after 6 months post-injury is the primary endpoint of the study. Secondary endpoints include routine blood chemistry parameters, the degree of spasticity, the ability to walk, urological function, quality of life, and the independence in everyday life.

Discussion

The application of ESWT activates the nervous tissue regeneration involving a multitude of various biochemical and cellular events and leads to a decreased neuronal loss. ESWT might contribute to an improvement in the treatment of acute traumatic SCI in future clinical use.

Trial registration

ClinicalTrials.govNCT04474106
Live birth after letrozole as an adjunct to follicle-stimulating hormone versus follicle-stimulating hormone alone for ovarian stimulation in in vitro fertilisation cycles—study protocol for a randomised controlled trial
Authors: Guo Wei , Li Hang Wun Raymond , Yang Zi , Zeng Lin , Yang Rui , Qiao Jie , Li Rong , Ng Ernest Hung Yu
Published: April 2022

Introduction

In vitro fertilisation (IVF) is an effective infertility treatment but the live birth rate remains unsatisfactory. Ovarian stimulation by follicle-stimulating hormone (FSH) is routinely used in IVF and the resulting high serum estradiol levels may impair oocyte/embryo quality and endometrial receptivity. Letrozole, an aromatase inhibitor, can reduce serum estradiol levels following ovarian stimulation. We aim to test the hypothesis that co-treatment with letrozole reduces supraphysiological serum estradiol levels and improves endometrial receptivity, leading to a higher live birth rate of IVF. We are conducting a randomised controlled trial (RCT) to evaluate whether letrozole as an adjunct to FSH in IVF is superior to FSH alone in the live birth rate of fresh embryo transfer.

Methods/design

This is an open-label randomised controlled superiority trial being performed in two assisted reproduction centres in China. Infertile women who have antral follicle count (AFC) before ovarian stimulation or on day 5 of ovarian stimulation ≥15 are randomly allocated in a 1:1 ratio to receive either letrozole and FSH or FSH alone in a GnRH antagonist protocol. Recruited women follow the standard operating procedures of the two centres. The primary outcome is the live birth rate of the fresh embryo transfer. Stimulation parameters, maternal side effects and obstetric and perinatal complications are secondary outcomes. The planned sample size is 900, i.e. 450 per group.

Discussion

The present study is the first multicentre randomised study to compare the live birth rate of the fresh embryo transfer following ovarian stimulation by letrozole and FSH versus FSH alone in women with anticipated high ovarian responses.

Trial registration

ClinicalTrials.gov NCT02912988. Registered on September 23, 2016. This trial protocol is version 2.0.
Are foxes (Vulpes spp.) good sentinel species for Toxoplasma gondii in northern Canada?
Authors: Bouchard Émilie , Sharma Rajnish , Hernández-Ortiz Adrián , Buhler Kayla , Al-Adhami Batol , Su Chunlei , Fenton Heather , G.-Gouin Géraldine , Roth James D. , Rodrigues Chloé Warret , Pamak Carla , Simon Audrey , Bachand Nicholas , Leighton Patrick , Jenkins Emily
Published: April 2022

Background

In changing northern ecosystems, understanding the mechanisms of transmission of zoonotic pathogens, including the coccidian parasite Toxoplasma gondii, is essential to protect the health of vulnerable animals and humans. As high-level predators and scavengers, foxes represent a potentially sensitive indicator of the circulation of T. gondii in environments where humans co-exist. The objectives of our research were to compare serological and molecular assays to detect T. gondii, generate baseline data on T. gondii antibody and tissue prevalence in foxes in northern Canada, and compare regional seroprevalence in foxes with that in people from recently published surveys across northern Canada.

Methods

Fox carcasses (Vulpes vulpes/Vulpes lagopus, n = 749) were collected by local trappers from the eastern (Labrador and Québec) and western Canadian Arctic (northern Manitoba, Nunavut, and the Northwest Territories) during the winters of 2015–2019. Antibodies in heart fluid were detected using a commercial enzyme-linked immunosorbent assay. Toxoplasma gondii DNA was detected in hearts and brains using a magnetic capture DNA extraction and real-time PCR assay.

Results

Antibodies against T. gondii and DNA were detected in 36% and 27% of foxes, respectively. Detection of antibodies was higher in older (64%) compared to younger foxes (22%). More males (36%) than females (31%) were positive for antibodies to T. gondii. Tissue prevalence in foxes from western Nunavik (51%) was higher than in eastern Nunavik (19%). At the Canadian scale, T. gondii exposure was lower in western Inuit regions (13%) compared to eastern Inuit regions (39%), possibly because of regional differences in fox diet and/or environment. Exposure to T. gondii decreased at higher latitude and in foxes having moderate to little fat. Higher mean infection intensity was observed in Arctic foxes compared to red foxes. Fox and human seroprevalence showed similar trends across Inuit regions of Canada, but were less correlated in the eastern sub-Arctic, which may reflect regional differences in human dietary preferences.

Conclusions

Our study sheds new light on the current status of T. gondii in foxes in northern Canada and shows that foxes serve as a good sentinel species for environmental circulation and, in some regions, human exposure to this parasite in the Arctic.

Graphical Abstract
Hurdles to breakthrough in CAR T cell therapy of solid tumors
Authors: Marofi Faroogh , Achmad Harun , Bokov Dmitry , Abdelbasset Walid Kamal , Alsadoon Zeid , Chupradit Supat , Suksatan Wanich , Shariatzadeh Siavash , Hasanpoor Zahra , Yazdanifar Mahboubeh , Shomali Navid , Khiavi Farhad Motavalli
Published: April 2022

Autologous T cells genetically engineered to express chimeric antigen receptor (CAR) have shown promising outcomes and emerged as a new curative option for hematological malignancy, especially malignant neoplasm of B cells. Notably, when T cells are transduced with CAR constructs, composed of the antigen recognition domain of monoclonal antibodies, they retain their cytotoxic properties in a major histocompatibility complex (MHC)-independent manner. Despite its beneficial effect, the current CAR T cell therapy approach faces myriad challenges in solid tumors, including immunosuppressive tumor microenvironment (TME), tumor antigen heterogeneity, stromal impediment, and tumor accessibility, as well as tribulations such as on-target/off-tumor toxicity and cytokine release syndrome (CRS). Herein, we highlight the complications that hamper the effectiveness of CAR T cells in solid tumors and the strategies that have been recommended to overcome these hurdles and improve infused T cell performance.
A survey on next location prediction techniques, applications, and challenges
Authors: Chekol Ayele Gobezie , Fufa Marta Sintayehu
Published: March 2022

Next location prediction has recently gained great attention from researchers due to its importance in different application areas. Recent growth of location-based service applications has vast domain influence such as traffic-flow prediction, weather forecast, and network resource optimization. Nowadays, due to the explosive increasing of positioning and sensor devices, big trajectory data are produced related to human movement. Using this big location-based trajectory data, researchers tend to predict human next location. Research efforts are spent on the put forward overall picture of next location prediction, and number of works has been done so as to realize robust next location prediction systems. However, in-depth study of those state-of-the-art works is required to know well the applications and challenges. Therefore, the aim of this paper is an extensive review on existing different next location prediction approaches. This work offers an extensive overview of location prediction enveloping basic definitions and concepts, data sources, approaches, and applications. In next location prediction, trajectory is represented by a sequence of timestamped geographical locations. It is challenging to analyze and mine trajectory data due to the complex characteristics reflected in human mobility, which is affected by multiple contextual information. Heterogeneous data generated from different sources, users’ random movement behavior, and the time sensitivity of trajectory data are some of the challenges. In this manuscript, we have discussed various location prediction approaches, applications, and challenges, and it sheds light on important points regarding future research directions. Furthermore, application and challenges are addressed related to the user’s next location prediction. Finally, we draw the overall conclusion of the survey, which is important for the development of robust next location prediction systems.
Effects of cadmium sulfide nanoparticles on sulfate bioreduction and oxidative stress in Desulfovibrio desulfuricans
Authors: Cheng Guoqing , Ding Huili , Chen Guanglin , Shi Hongjie , Zhang Xu , Zhu Minglong , Tan Wensong
Published: April 2022

Sulfate-containing wastewater has a serious threat to the environment and human health. Microbial technology has great potential for the treatment of sulfate-containing wastewater. It was found that nano-photocatalysts could be used as extracellular electron donors to promote the growth and metabolic activity of non-photosynthetic microorganisms. However, nano-photocatalysts could also induce oxidative stress and damage cells. Therefore, the interaction mechanism between photosynthetic nanocatalysts and non-photosynthetic microorganisms is crucial to determine the regulatory strategies for microbial wastewater treatment technologies. In this paper, the mechanism and regulation strategy of cadmium sulfide nanoparticles (CdS NPs) on the growth of sulfate-reducing bacteria and the sulfate reduction process were investigated. The results showed that the sulfate reduction efficiency could be increased by 6.4% through CdS NPs under light conditions. However, the growth of Desulfovibrio desulfuricans C09 was seriously inhibited by 55% due to the oxidative stress induced by CdS NPs on cells. The biomass and sulfate reduction efficiency could be enhanced by 6.8% and 5.9%, respectively, through external addition of humic acid (HA). At the same time, the mechanism of the CdS NPs strengthening the sulfate reduction process by sulfate bacteria was also studied which can provide important theoretical guidance and technical support for the development of microbial technology combined with extracellular electron transfer (EET) for the treatment of sulfate-containing wastewater.

Graphical Abstract
Paleotsunami history of Hachinohe, northern Japan: a multiproxy analysis and numerical modeling approach
Authors: Velasco-Reyes Erick R. , Goto Kazuhisa , Sugawara Daisuke , Nishimura Yuichi , Shinohara Takahiro , Chiba Takashi
Published: April 2022

Paleotsunami studies along the Pacific coast of Tohoku, northern Japan, have been considerably developed recently, particularly after the massive impact of the 2011 Tohoku-oki tsunami. Nevertheless, in the southernmost Shimokita Peninsula, studies pertaining to paleotsunami are underdeveloped, leading to a vague understanding of the tsunamigenic sources northward of the Tohoku region, along with incomplete hazard evaluation. Paleotsunami deposits in Shimokita can be related not only to the Japan Trench along the Sanriku coast but also to the Kuril trench along the Pacific coast of Hokkaido. In this study, we unveiled the paleotsunami history of Hachinohe in northern Tohoku. Using a combination of sedimentological, geochemical, paleontological, and mineralogical proxies, we characterized seven sand layers that dated from ca. 2700 to ca. 5500 yr BP based on radiocarbon (14C) ages as event deposits of marine origin. Sedimentological and paleontological evidence coupled with ground-penetrating radar imagery revealed a marsh environment comprising successive extinct ponds, controlling the depositional environment. Numerical modeling ruled out the possibility of storms as genetic sources, leading to the conclusion that the presence of event deposits with marine sediments in the study area would be associated with tsunami inundation episodes. Based on 14C dating, the mean frequency of recurrence of tsunamis is estimated as 384 years (320–450 yr, 95% confidence interval) and a coefficient of variation of 0.78 (0.68–0.99, 95% confidence interval). The previously recorded limited paleotsunami evidence and absence of an estimated recurrence interval in the Shimokita Peninsula reaffirm the importance of Hachinohe as a tsunami record site for the activity of both trenches.
Automated detection of myopic maculopathy from color fundus photographs using deep convolutional neural networks
Authors: Li Jun , Wang Lilong , Gao Yan , Liang Qianqian , Chen Lingzhi , Sun Xiaolei , Yang Huaqiang , Zhao Zhongfang , Meng Lina , Xue Shuyue , Du Qing , Zhang Zhichun , Lv Chuanfeng , Xu Haifeng , Guo Zhen , Xie Guotong , Xie Lixin
Published: April 2022

Background

Myopic maculopathy (MM) has become a major cause of visual impairment and blindness worldwide, especially in East Asian countries. Deep learning approaches such as deep convolutional neural networks (DCNN) have been successfully applied to identify some common retinal diseases and show great potential for the intelligent analysis of MM. This study aimed to build a reliable approach for automated detection of MM from retinal fundus images using DCNN models.

Methods

A dual-stream DCNN (DCNN-DS) model that perceives features from both original images and corresponding processed images by color histogram distribution optimization method was designed for classification of no MM, tessellated fundus (TF), and pathologic myopia (PM). A total of 36,515 gradable images from four hospitals were used for DCNN model development, and 14,986 gradable images from the other two hospitals for external testing. We also compared the performance of the DCNN-DS model and four ophthalmologists on 3000 randomly sampled fundus images.

Results

The DCNN-DS model achieved sensitivities of 93.3% and 91.0%, specificities of 99.6% and 98.7%, areas under the receiver operating characteristic curves (AUC) of 0.998 and 0.994 for detecting PM, whereas sensitivities of 98.8% and 92.8%, specificities of 95.6% and 94.1%, AUCs of 0.986 and 0.970 for detecting TF in two external testing datasets. In the sampled testing dataset, the sensitivities of four ophthalmologists ranged from 88.3% to 95.8% and 81.1% to 89.1%, and the specificities ranged from 95.9% to 99.2% and 77.8% to 97.3% for detecting PM and TF, respectively. Meanwhile, the DCNN-DS model achieved sensitivities of 90.8% and 97.9% and specificities of 99.1% and 94.0% for detecting PM and TF, respectively.

Conclusions

The proposed DCNN-DS approach demonstrated reliable performance with high sensitivity, specificity, and AUC to classify different MM levels on fundus photographs sourced from clinics. It can help identify MM automatically among the large myopic groups and show great potential for real-life applications.
Sub-acute Changes on MRI Measures of Cerebral Blood Flow and Venous Oxygen Saturation in Concussed Australian Rules Footballers
Authors: Wright David K. , O’Brien Terence J. , Shultz Sandy R.
Published: April 2022

Background

Sports-related concussion (SRC) is common in collision sport athletes. There is growing evidence that repetitive SRC can have serious neurological consequences, particularly when the repetitive injuries occur when the brain has yet to fully recover from the initial injury. Hence, there is a need to identify biomarkers that are capable of determining SRC recovery so that they can guide clinical decisions pertaining to return-to-play. Cerebral venous oxygen saturation (SvO2) and cerebral blood flow (CBF) can be measured using magnetic resonance imaging (MRI) and may provide insights into changing energy demands and recovery following SRC.

Results

In this study we therefore investigated SvO2and CBF in a cohort of concussed amateur Australian Football athletes (i.e., Australia’s most participated collision sport). Male and female Australian footballers (n = 13) underwent MRI after being cleared to return to play following a mandatory 13-day recovery period and were compared to a group of control Australian footballers (n = 16) with no recent history of SRC (i.e., > 3 months since last SRC). Despite the concussed Australian footballers being cleared to return to play at the time of MRI, we found evidence of significantly increased susceptibility in the global white matter (p = 0.020) and a trend (F5,21 = 2.404,p = 0.071) for reduced relative CBF (relCBF) compared to the control group. Further, there was evidence of an interaction between sex and injury in straight sinus susceptibility values (F1,25 = 3.858,p = 0.061) which were decreased in female SRC athletes (p = 0.053). Of note, there were significant negative correlations between straight sinus susceptibility and relCBF suggesting impaired metabolic function after SRC.

Conclusions

These findings support the use of quantitative susceptibility mapping (QSM) and relCBF as sensitive indicators of SRC, and raise further concerns related to SRC guidelines that allow for return-to-play in less than two weeks.
Zamzam water may act as pesticide with and without globe artichoke extract against the cotton mealy bug, Phenacoccus solenopsis
Authors: Abd-Allah Ghada E.
Published: April 2022

Background

The holy water, Zamzam has many benefits to the human body. Therefore, the present study aims to clear new use of Zamzam water that it can be used as pesticide. In this study, Zamzam water was tested as pesticide alone and with globe artichoke extract and the globe artichoke extract also with tap water against the cotton mealybug, Phenacoccus solenopsis.

Results

The obtained results proved that, Zamzam water only has mortality rate approaches the mortality rate of the globe artichoke extract alone. Zamzam water achieved 60% mortality against the adult, P. solenopsis. While 20.000 ppm globe artichoke extract with tap water achieved 70% mortality. Zamzam water, also, improved the effect of globe artichoke extract when diluted with it and the mortality increased to be 86.67% at 15,000 ppm. Likewise, LC50 was 2055.4 ppm with globe artichoke extract with Zamzam water, while it was 13,494.8 ppm with globe artichoke extract without Zamzam water. However, LC90 was 35,791.5 ppm with globe artichoke extract with Zamzam water while it was 48,922.9 ppm with globe artichoke extract without Zamzam water. Also, the chemical analysis of Zamzam water proved the difference in its composition than tap water especially in cations and anions that causes properties like magnetism.

Conclusions

Based on the obtained results, the globe artichoke extract with Zamzam water was the most toxic in control of P. solenopsis. This may open great attention to the other uses of the holy water, Zamzam water in pests’ management and encourage more researches about globe artichoke leaves extract and Zamzam water.
An ecological study of freshwater ecosystem and its colligation to Odonates assemblages in Ipogun, Southwest Nigeria
Authors: Adu Babasola , Dada Omolola , Tunwase Victor
Published: April 2022

Background

Odonata (dragonfly and damselfly) are particularly good indicators of freshwater ecosystem health. The constant disturbance of freshwater habitats can result in the reduction of Odonata species diversity. Changes in Odonata biodiversity are influenced by several human activities, such as agriculture, urbanization, input of pollutants in water and construction. This study was carried out to assess the abundance and diversity of Odonata, evaluate the physicochemical characteristics of water, and compare the community structure of Odonata at three selected sites along River Aponmu in Ipogun. Adult odonates were sampled and identified for 11 months using a sweep net, water samples were collected and some parameters were determined during the study period.

Results

A total of 906 specimens representing sixty-four (64) species and sixteen (16) genera in seven (7) families (Coenagrionidae, Lestidae, Platycnemididae, Chlorocyphidae, Calopterygidae, Libellulidae, and Gomphidae) were collected and identified. Of the 906 specimens, Libellulidae had the highest percentage composition (44%) with 395 individuals out of which Trithemis arteriosa (a pollution tolerant species) had the highest number of individuals (225) and Gomphidae had the lowest percentage composition (0.03%) with 1 individual. Most of the species collected are known for their tolerance to disturbed environments. They include Pseudagrion melanicterum, Paragomphus genei, and Orthetrum Julia. Aponmu area had the highest species diversity (H′ = 2.312) while Idi area had the least species diversity (H′ = 2.021). Alaasin area had the highest Simpson_d value (0.8557) and the best taxa distribution (Evenness = 0.524; Equitability_J = 0.7764) which makes the area more pristine than other sites while Aponmu area had the least distribution (Evenness = 0.3365; Equitability_J = 0.6798). Analysis of variance (ANOVA) result of physicochemical parameters revealed that temperature (°C), pH, Dissolved Oxygen (mg/L), turbidity (NTU), Biochemical Oxygen Demand (mg/L), NO3 (mg/L), and PO4 (mg/L) did not show significant difference at the three sites while EC (µS/cm) and TDS (mg/L) which have moderately high mean values indicated significant difference at Aponmu area (p < 0.05). T. arteriosa exhibited a weak negative correlation to both temperature and DO.

Conclusions

This study has provided information on Odonata assemblage at River Aponmu and infers based on the assemblage that the river may be somewhat polluted at the period the research was carried out. It is therefore recommended that efforts should therefore be taken to discourage water pollution in order to preserve the diversity of these insects and the water quality.
Ground-penetrating radar attenuation compensation by Gabor deconvolution: Seismogenic fault imaging at Castelluccio di Norcia (Central Italy)
Authors: Ercoli Maurizio , Ferguson Robert J.
Published: March 2022

We test a seismic nonstationary Gabor deconvolution (GD) algorithm on synthetic and experimental ground-penetrating radar (GPR) profiles to evaluate how well this algorithm increases vertical resolution and removes attenuation effects from GPR data. Our field data set has been collected across a seismogenic fault in Central Italy, detecting this tectonic structure several years before the 2016–2017 seismic sequence which struck the region and produced coseismic ruptures along the same fault trace. We find that GPR mixed-phase data respond very well to the application of GD in comparison with the conventional and more standard Wiener-spiking deconvolution workflows. We observe a clear increase of the coherence and sharpness of reflection events as well as of hyperbolic diffractions in the fault zone. Gabor-processed GPR data significantly increase the GPR potential to image active Quaternary faults, therefore contributing to the definition of seismotectonic context and to seismic hazard assessment of a study region. We propose the use of the GD to increase interpretability of GPR profiles not only for the identification of tectonic structures but also to achieve high-quality images of the near surface in many GPR applications.
The potential of precision psychiatry: what is in reach?
Authors: Kambeitz-Ilankovic Lana , Koutsouleris Nikolaos , Upthegrove Rachel
Published: March 2022

Progress in developing personalised care for mental disorders is supported by numerous proof-of-concept machine learning studies in the area of risk assessment, diagnostics and precision prescribing. Most of these studies primarily use clinical data, but models might benefit from additional neuroimaging, blood and genetic data to improve accuracy. Combined, multimodal models might offer potential for stratification of patients for treatment. Clinical implementation of machine learning is impeded by a lack of wider generalisability, with efforts primarily focused on psychosis and dementia. Studies across all diagnostic groups should work to test the robustness of machine learning models, which is an essential first step to clinical implementation, and then move to prospective clinical validation. Models need to exceed clinicians’ heuristics to be useful, and safe, in routine decision-making. Engagement of clinicians, researchers and patients in digitalisation and ‘big data’ approaches are vital to allow the generation and accessibility of large, longitudinal, prospective data needed for precision psychiatry to be applied into real-world psychiatric care.
Identification of High-Risk Multiple Myeloma With a Plasma Cell Leukemia-Like Transcriptomic Profile
Authors: Hofste op Bruinink Davine , Kuiper Rowan , van Duin Mark , Cupedo Tom , van der Velden Vincent H.J. , Hoogenboezem Remco , van der Holt Bronno , Beverloo H. Berna , Valent Erik T. , Vermeulen Michael , Gay Francesca , Broijl Annemiek , Avet-Loiseau Hervé , Munshi Nikhil C. , Musto Pellegrino , Moreau Philippe , Zweegman Sonja , van de Donk Niels W.C.J. , Sonneveld Pieter
Published: March 2022

PURPOSE

Primary plasma cell leukemia (pPCL) is an aggressive subtype of multiple myeloma, which is distinguished from newly diagnosed multiple myeloma (NDMM) on the basis of the presence of ≥ 20% circulating tumor cells (CTCs). A molecular marker for pPCL is currently lacking, which could help identify NDMM patients with high-risk PCL-like disease, despite not having been recognized as such clinically.

METHODS

A transcriptomic classifier for PCL-like disease was bioinformatically constructed and validated by leveraging information on baseline CTC levels, tumor burden, and tumor transcriptomics from 154 patients with NDMM included in the Cassiopeia or HO143 trials and 29 patients with pPCL from the EMN12/HO129 trial. Its prognostic value was assessed in an independent cohort of 2,139 patients with NDMM from the HOVON-65/GMMG-HD4, HOVON-87/NMSG-18, EMN02/HO95, MRC-IX, Total Therapy 2, Total Therapy 3, and MMRF CoMMpass studies.

RESULTS

High CTC levels were associated with the expression of 1,700 genes, independent of tumor burden (false discovery rate < 0.05). Of these, 54 genes were selected by leave-one-out cross-validation to construct a transcriptomic classifier representing PCL-like disease. This not only demonstrated a sensitivity of 93% to identify pPCL in the validation cohort but also classified 10% of NDMM tumors as PCL-like. PCL-like MM transcriptionally and cytogenetically resembled pPCL, but presented with significantly lower CTC levels and tumor burden. Multivariate analyses in NDMM confirmed the significant prognostic value of PCL-like status in the context of Revised International Staging System stage, age, and treatment, regarding both progression-free (hazard ratio, 1.64; 95% CI, 1.30 to 2.07) and overall survival (hazard ratio, 1.89; 95% CI, 1.42 to 2.50).

CONCLUSION

pPCL was identified on the basis of a specific tumor transcriptome, which was also present in patients with high-risk NDMM, despite not being clinically leukemic. Incorporating PCL-like status into current risk models in NDMM may improve prognostic accuracy.
Online Medical Misinformation in Cancer: Distinguishing Fact From Fiction
Authors: Teplinsky Eleonora , Ponce Sara Beltrán , Drake Emily K. , Garcia Ann Meredith , Loeb Stacy , van Londen G.J. , Teoh Deanna , Thompson Michael , Schapira Lidia ,   for the Collaboration for Outcomes using Social Media in Oncology (COSMO)
Published: March 2022

It is without question that the Internet has democratized access to medical information, with estimates that 70% of the American population use it as a resource, particularly for cancer-related information. Such unfettered access to information has led to an increase in health misinformation. Fortunately, the data indicate that health care professionals remain among the most trusted information resources. Therefore, understanding how the Internet has changed engagement with health information and facilitated the spread of misinformation is an important task and challenge for cancer clinicians. In this review, we perform a meta-synthesis of qualitative data and point toward empirical evidence that characterizes misinformation in medicine, specifically in oncology. We present this as a call to action for all clinicians to become more active in ongoing efforts to combat misinformation in oncology.