bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Trimethylamine N-Oxide on Platelet Activation
Authors: Emonds Julian Josef , Ringel Clemens , Reinicke Madlen , Müller Daniel , Von Eckardstein Arnold , Meixensberger Jürgen , Ceglarek Uta , Gaudl Alexander
Published: August 2022

Microbiome-derived trimethylamine N-oxide (TMAO) has been associated with platelet hyperreactivity and subsequent atherogenesis. Whether physiological TMAO-levels influence platelet-derived lipid mediators remains unknown. Little is known about pre-analytic factors potentially influencing TMAO concentrations. We aimed at developing a quantitative LC-MS/MS method to investigate in-vivo and in-vitro pre-analytical factors in TMAO analysis to properly assess the proposed activating effect of TMAO on platelets. TMAO, betaine, carnitine, and choline were analyzed by HILIC-ESI-MS/MS within 6 min total run time. Method validation included investigation of reproducibility, recovery, sensitivity, and in-vitro pre-analytical factors. A 24-h monitoring experiment was performed, evaluating in-vivo pre-analytical factors like daytime or diet. Finally, the effects of different TMAO concentrations on platelet activation and corresponding alterations of platelet-derived eicosanoid release were analyzed. The method showed high reproducibility (CVs ≤ 5.3%), good recovery rates (96–98%), and negligible in-vitro pre-analytical effects. The influence of in-vivo pre-analytical factors on TMAO levels was not observable within the applied experimental conditions. We did not find any correlation between TMAO levels and platelet activation at physiological TMAO concentrations, whereas platelet-derived eicosanoids presented activation of the cyclooxygenase and lipoxygenase pathways. In contrast to previously published results, we did not find any indications regarding diet dependency or circadian rhythmicity of TMAO levels. Our results do not support the hypothesis that TMAO increases platelet responsiveness via the release of lipid-mediators.
Comparative Pathology of Zoonotic Orthopoxviruses
Authors: MacNeill Amy L.
Published: August 2022

This review provides a brief history of the impacts that a human-specific Orthopoxvirus (OPXV), Variola virus, had on mankind, recalls how critical vaccination was for the eradication of this disease, and discusses the consequences of discontinuing vaccination against OPXV. One of these consequences is the emergence of zoonotic OPXV diseases, including Monkeypox virus (MPXV). The focus of this manuscript is to compare pathology associated with zoonotic OPXV infection in veterinary species and in humans. Efficient recognition of poxvirus lesions and other, more subtle signs of disease in multiple species is critical to prevent further spread of poxvirus infections. Additionally included are a synopsis of the pathology observed in animal models of MPXV infection, the recent spread of MPXV among humans, and a discussion of the potential for this virus to persist in Europe and the Americas.
A Retrospective Review on Severe Malaria in Colombia, 2007–2020
Authors: Carmona-Fonseca Jaime , Olivera Mario J. , Yasnot-Acosta María F.
Published: August 2022

Background: Knowledge of severe malaria (SM) or complicated malaria is insufficient in all its components. The least known type is the one associated with Plasmodium vivax, compared to that caused by P. falciparum. The aim of this study was to provide a general overview of epidemiological information about the burden of SM, obtained from the National Public Health Surveillance System (SIVIGILA) for the period 2007–2020 in Colombia. Methods: A descriptive, retrospective, and cross-sectional study of secondary information was performed via SIVIGILA. Results: There were 9881 SM cases among 1,060,950 total malaria cases in Colombia in 2007–2020: 9.31 SM cases per 1000 malaria cases. During this period, there were 7145 SM cases due to the following species: Plasmodium vivax, 57.6%; P. falciparum, 38.6%; severe mixed malaria, 3.2%; and P. malariae, 0.6%. The most compromised organ systems are the hematological system (54.9%), the liver (9.1%), the kidneys (4.2%), the lungs (1.9%) and the brain (1.6%). Conclusions: There has been a reduction in malaria incidence in Colombia in the last 10–15 years, but there has also been a strong increase in SM incidence. We suggest emphasizing the prevention of the onset of severe malaria, with the early and accurate diagnosis of plasmodial infection.
ELISA Test Based on the Phenolic Glycolipid-I (PGL-I) of Mycobacterium leprae: A Reality of a Laboratory from a Non-Endemic Country
Authors: Longoni Silvia Stefania , Beltrame Anna , Prato Marco , Spencer John Stewart , Bergamaschi Nicolo , Clapasson Andrea , Parodi Aurora , Piubelli Chiara , Perandin Francesca
Published: August 2022

Background: Leprosy is a neglected tropical disease caused by Mycobacterium leprae, leading to disabilities if untreated. The ELISA based on phenolic glycolipid I (PGL-I), or its synthetic version ND-O-BSA, is almost universally positive in multibacillary leprosy and thus extensively used in endemic countries. Household contacts with a positive antibody titer have ~6-fold higher probability to develop the disease than those with a negative titer. Thus, the aim of the study was to evaluate the performance of this ELISA in the setting of a non-endemic country. Methods: We calculate the cut-off using optimized O.D. thresholds, generated by receiver operating characteristics (ROC) curve analysis, testing 39 well-characterized sera obtained from lepromatous leprosy patients with strongly positive ND-O-BSAELISA titer and 39 sera from healthy non-endemic patients never exposed to M. leprae or M. tuberculosis. Indeed, we tested a second set of sera from suspected or confirmed leprosy or household contacts (SLALT group, n=50), and patients with tuberculosis (control group, n=40). Results: We detected 56.4% of SLALT and 22.5% of tuberculosis as positive, consistent with the literature. Conclusion: The ELISA based on ND-O-BSA may thus be considered a good option to be used in a non-endemic area as a screening tool in at risk population usually coming to our center.
Robot-Assisted Ankle Rehabilitation Using the Hybrid Assistive Limb for Children after Equinus Surgery: A Report of Two Cases
Authors: Takahashi Kazushi , Mutsuzaki Hirotaka , Yoshikawa Kenichi , Yamamoto Satoshi , Koseki Kazunori , Takeuchi Ryoko , Mataki Yuki , Iwasaki Nobuaki
Published: August 2022

After equinus corrective surgery, repetitive exercises for ankle dorsiflexion and plantar flexion are crucial during rehabilitation. The single-joint Hybrid Assistive Limb (HAL-SJ) is an advanced exoskeletal robotic device with a control system that uses bioelectrical signals to assist joint motion in real time and demonstrates joint torque assistance with the wearer’s voluntary movement. We present two cases of robot-assisted ankle rehabilitation after equinus surgery using the HAL-SJ in children. Case 1 was an 8-year-old boy, whereas case 2 was a 6-year-old boy. When they were allowed to walk without braces, training with the HAL-SJ was performed postoperatively for 20 min per session a total of eight times (2–4 sessions per week). Assessments were performed before and after HAL-SJ training. During gait analysis, case 1 had improved joint angles during the stance phase on the operated side; however, case 2 had improved joint angles during the stance and swing phases. The co-activation index values of the medial gastrocnemius and tibialis anterior muscles, which were high before training, decreased after training and approached the standard value. The HAL-SJ may provide systematic feedback regarding voluntary ankle dorsiflexion and plantar flexion and is considered to have motor learning effects.
Evaluation of the Cardiac Electrophysiological and Haemodynamic Effects of Elsholtzia ciliata Essential Oil on Swine
Authors: Zigmantaitė Vilma , Jonušaitė Eglė , Grigalevičiūtė Ramunė , Kučinskas Audrius , Treinys Rimantas , Navalinskas Antanas , Žvikas Vaidotas , Jakštas Valdas , Pudžiuvelytė Lauryna , Bernatonienė Jurga , Mačianskienė Regina , Jurevičius Jonas
Published: August 2022

The demand for the development of novel medicines with few side effects and no proarrhythmic properties is increasing. Extensive research on herbal extracts has been conducted with the expectation that the compounds will exert precise effects without harmful side effects. Elsholtzia ciliata (Thunb.) Hyl. essential oil (EO) possesses antiarrhythmic properties similar to those of class 1B antiarrhythmics, such as prolonging myocardial activation of the QRS complex and shortening the QT interval. In this study, we determined the kinetic profile of EO phytocompounds and the effects of EO on heart electrical activity and arterial blood pressure. For this study, we chose to use local breed pigs that were anaesthetized. The effects of an intravenous bolus of EO on ECG parameters, arterial blood pressure, heart rate variability, and blood levels of haematological and biochemical parameters were registered and evaluated. Following an intravenous injection of a bolus, EO exerted a vasodilatory effect, resulting in significant reductions in arterial blood pressure. EO also increased the heart rate and altered ECG parameters. The bolus of EO prolonged the QRS complex, shortened the QT interval, and nonmonotonically altered the PQ interval. After the administration of a bolus of EO, the activity of the autonomic nervous system was altered. This study confirms that EO possesses similar properties to class 1B antiarrhythmics and exerts a hypotensive effect; it reduces arterial blood pressure possibly by modulating peripheral vascular resistance.
Total Synthesis of the Antimycobacterial Natural Product Chlorflavonin and Analogs via a Late-Stage Ruthenium(II)-Catalyzed ortho-C(sp2)-H-Hydroxylation
Authors: Berger Alexander , Knak Talea , Kiffe-Delf Anna-Lene , Mudrovcic Korana , Singh Vinayak , Njoroge Mathew , Burckhardt Bjoern B. , Gopalswamy Mohanraj , Lungerich Beate , Ackermann Lutz , Gohlke Holger , Chibale Kelly , Kalscheuer Rainer , Kurz Thomas
Published: August 2022

The continuous, worldwide spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) endanger the World Health Organization’s (WHO) goal to end the global TB pandemic by the year 2035. During the past 50 years, very few new drugs have been approved by medical agencies to treat drug-resistant TB. Therefore, the development of novel antimycobacterial drug candidates to combat the threat of drug-resistant TB is urgent. In this work, we developed and optimized a total synthesis of the antimycobacterial natural flavonoid chlorflavonin by selective ruthenium(II)-catalyzed ortho-C(sp2)-H-hydroxylation of a substituted 3′-methoxyflavonoid skeleton. We extended our methodology to synthesize a small compound library of 14 structural analogs. The new analogs were tested for their antimycobacterial in vitro activity against Mycobacterium tuberculosis (Mtb) and their cytotoxicity against various human cell lines. The most promising new analog bromflavonin exhibited improved antimycobacterial in vitro activity against the virulent H37Rv strain of Mtb (Minimal Inhibitory Concentrations (MIC90) = 0.78 μm). In addition, we determined the chemical and metabolic stability as well as the pKa values of chlorflavonin and bromflavonin. Furthermore, we established a quantitative structure–activity relationship model using a thermodynamic integration approach. Our computations may be used for suggesting further structural changes to develop improved derivatives.
Comparison of the Blood–Brain Barrier Transport and Vulnerability to P-Glycoprotein-Mediated Drug–Drug Interaction of Domperidone versus Metoclopramide Assessed Using In Vitro Assay and PET Imaging
Authors: Breuil Louise , Goutal Sébastien , Marie Solène , Del Vecchio Antonio , Audisio Davide , Soyer Amélie , Goislard Maud , Saba Wadad , Tournier Nicolas , Caillé Fabien
Published: August 2022

Domperidone and metoclopramide are widely prescribed antiemetic drugs with distinct neurological side effects. The impact of P-glycoprotein (P-gp)-mediated efflux at the blood–brain barrier (BBB) on brain exposure and BBB permeation was compared in vitro and in vivo using positron emission tomography (PET) imaging in rats with the radiolabeled analogs [11C]domperidone and [11C]metoclopramide. In P-gp-overexpressing cells, the IC50 of tariquidar, a potent P-gp inhibitor, was drastically different using [11C]domperidone (221 nM [198–248 nM]) or [11C]metoclopramide (4 nM [2–8 nM]) as the substrate. Complete P-gp inhibition led to a 1.8-fold higher increase in the cellular uptake of [11C]domperidone compared with [11C]metoclopramide (p < 0.0001). Brain PET imaging revealed that the baseline brain exposure (AUCbrain) of [11C]metoclopramide was 2.4-fold higher compared with [11C]domperidone (p < 0.001), consistent with a 1.8-fold higher BBB penetration (AUCbrain/AUCplasma). The maximal increase in the brain exposure (2.9-fold, p < 0.0001) and BBB penetration (2.9-fold, p < 0.0001) of [11C]metoclopramide was achieved using 8 mg/kg of tariquidar. In comparison, neither 8 nor 15 mg/kg of tariquidar increased the brain exposure of [11C]domperidone (p > 0.05). Domperidone is an avid P-gp substrate that was in vitro compared with metoclopramide. Domperidone benefits from a lower brain exposure and a limited risk for P-gp-mediated drug–drug interaction involving P-gp inhibition at the BBB.
PSME4 Degrades Acetylated YAP1 in the Nucleus of Mesenchymal Stem Cells
Authors: Kim Yong Sook , Kim Mira , Cho Dong Im , Lim Soo Yeon , Jun Ju Hee , Kim Mi Ra , Kang Bo Gyeong , Eom Gwang Hyeon , Kang Gaeun , Yoon Somy , Ahn Youngkeun
Published: August 2022

Intensive research has focused on minimizing the infarct area and stimulating endogenous regeneration after myocardial infarction. Our group previously elucidated that apicidin, a histone deacetylase (HDAC) inhibitor, robustly accelerates the cardiac commitment of naïve mesenchymal stem cells (MSCs) through acute loss of YAP1. Here, we propose the novel regulation of YAP1 in MSCs. We found that acute loss of YAP1 after apicidin treatment resulted in the mixed effects of transcriptional arrest and proteasomal degradation. Subcellular fractionation revealed that YAP1 was primarily localized in the cytoplasm. YAP1 was acutely relocalized into the nucleus and underwent proteasomal degradation. Interestingly, phosphor-S127 YAP1 was shuttled into the nucleus, suggesting that a mechanism other than phosphorylation governed the subcellular localization of YAP1. Apicidin successfully induced acetylation and subsequent dissociation of YAP1 from 14-3-3, an essential molecule for cytoplasmic restriction. HDAC6 regulated both acetylation and subcellular localization of YAP1. An acetylation-dead mutant of YAP1 retarded nuclear redistribution upon apicidin treatment. We failed to acquire convincing evidence for polyubiquitination-dependent degradation of YAP1, suggesting that a polyubiquitination-independent regulator determined YAP1 fate. Nuclear PSME4, a subunit of the 26 S proteasome, recognized and degraded acetyl YAP1 in the nucleus. MSCs from PSME4-null mice were injected into infarcted heart, and aberrant sudden death was observed. Injection of immortalized human MSCs after knocking down PSME4 failed to improve either cardiac function or the fibrotic scar area. Our data suggest that acetylation-dependent proteasome subunit PSME4 clears acetyl-YAP1 in response to apicidin treatment in the nucleus of MSCs.
Atorvastatin Enhances the Efficacy of Immune Checkpoint Therapy and Suppresses the Cellular and Extracellular Vesicle PD-L1
Authors: Choe Eun-Ji , Lee Chan-Hyeong , Bae Ju-Hyun , Park Ju-Mi , Park Seong-Sik , Baek Moon-Chang
Published: August 2022

According to clinical studies, statins improve the efficacy of programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) blockade therapy for breast cancer; however, the underlying mechanisms are unclear. Herein, we showed that atorvastatin (ATO) decreased the content of PD-L1 in extracellular vesicles (EVs) by reducing cellular PD-L1 expression and inhibiting EV secretion in breast cancer cells, thereby enhancing the efficacy of anti-PD-L1 therapy. ATO reduced EV secretion by regulating the Rab proteins involved in EV biogenesis and secretion. ATO-mediated inhibition of the Ras-activated MAPK signaling pathway downregulated PD-L1 expression. In addition, ATO strongly promoted antitumor efficacy by inducing T cell-mediated tumor destruction when combined with an anti-PD-L1 antibody. Moreover, suppression of EV PD-L1 by ATO improved the reactivity of anti-PD-L1 therapy by enhancing T-cell activity in draining lymph nodes of EMT6-bearing immunocompetent mice. Therefore, ATO is a potential therapeutic drug that improves antitumor immunity by inhibiting EV PD-L1, particularly in response to immune escape during cancer.
Assessment of Bacterial Nanocellulose Loaded with Acetylsalicylic Acid or Povidone-Iodine as Bioactive Dressings for Skin and Soft Tissue Infections
Authors: Argel Shaydier , Castaño Melissa , Jimenez Daiver Estiven , Rodríguez Sebastian , Vallejo Maria Jose , Castro Cristina Isabel , Osorio Marlon Andres
Published: August 2022

Bacterial nanocellulose (BNC) is a novel nanomaterial known for its large surface area, biocompatibility, and non-toxicity. BNC contributes to regenerative processes in the skin but lacks antimicrobial and anti-inflammatory properties. Herein, the development of bioactive wound dressings by loading antibacterial povidone-iodine (PVI) or anti-inflammatory acetylsalicylic acid (ASA) into bacterial cellulose is presented. BNC is produced using Hestrin–Schramm culture media and loaded via immersion in PVI and ASA. Through scanning electron microscopy, BNC reveals open porosity where the bioactive compounds are loaded; the mechanical tests show that the dressing prevents mechanical wear. The loading kinetic and release assays (using the Franz cell method) under simulated fluids present a maximum loading of 589.36 mg PVI/g BNC and 38.61 mg ASA/g BNC, and both systems present a slow release profile at 24 h. Through histology, the complete diffusion of the bioactive compounds is observed across the layers of porcine skin. Finally, in the antimicrobial experiment, BNC/PVI produced an inhibition halo for Gram-positive and Gram-negative bacteria, confirming the antibacterial activity. Meanwhile, the protein denaturation test shows effective anti-inflammatory activity in BNC/ASA dressings. Accordingly, BNC is a suitable platform for the development of bioactive wound dressings, particularly those with antibacterial and anti-inflammatory properties.
Effects of a Whole Plant Extract of Scutellaria rubropunctata var. rubropunctata on Bone Metabolism with Estrogen Receptor Activation
Authors: Watanabe Misaki , Yahagi Tadahiro , Shirayama Takahiro , Miyake Katsunori , Kotani Hitoshi , Ogawa Takuya , Matsuzaki Keiichi
Published: August 2022

We screened natural resources for estrogen receptor (ER)-activating and bone metabolism-promoting activities with the aim of finding potential treatments for osteoporosis. A screen of 1531 extracts from Ryukyu Arc plants using a luciferase reporter assay identified an 80% MeOH extract of Scutellaria rubropunctata var. rubropunctata (SRE) with dose-dependent ER transcription-promoting activity. Importantly, SRE had no proliferative effect on human breast cancer cells. SRE enhanced the ALP activity of pre-osteoblast MC3T3-E1 cells after 72 h in culture and slightly enhanced mineralization at 14 and 21 d. SRE did not significantly affect the TRAP activity of RAW264.7 cells. Gene expression analysis in MC3T3-E1 cells by quantitative real-time PCR revealed that SRE upregulated the mRNA levels of Runx2, Osterix (Osx), Osteopontin (Opn), Osteocalcin (Ocn), Smad1, Smad4, and Smad5 at 72 h, and those of Runx2, Osx, Smad1, Smad4, and Smad5 at 21 d of osteogenic induction. Analysis of the expression levels of osteogenic markers suggested that SRE may promote osteogenic differentiation by acting at the early stage of differentiation rather than at the late stage of differentiation. These results indicate that SRE activates ER and induces osteoblast differentiation by activating Runx2 and Osx through the BMP/Smad pathway, suggesting that SRE may be useful for the prevention and treatment of postmenopausal osteoporosis.
A Locus Controlling Leaf Rolling Degree in Wheat under Drought Stress Identified by Bulked Segregant Analysis
Authors: Yang Xi , Wang Jingyi , Mao Xinguo , Li Chaonan , Li Long , Xue Yinghong , He Liheng , Jing Ruilian
Published: August 2022

Drought stress frequently occurs, which seriously restricts the production of wheat (Triticum aestivum L.). Leaf rolling is a typical physiological phenomenon of plants during drought stress. To understand the genetic mechanism of wheat leaf rolling, we constructed an F2 segregating population by crossing the slight-rolling wheat cultivar “Aikang 58” (AK58) with the serious-rolling wheat cultivar ″Zhongmai 36″ (ZM36). A combination of bulked segregant analysis (BSA) with Wheat 660K SNP Array was used to identify molecular markers linked to leaf rolling degree. A major locus for leaf rolling degree under drought stress was detected on chromosome 7A. We named this locus LEAF ROLLING DEGREE 1 (LERD1), which was ultimately mapped to a region between 717.82 and 720.18 Mb. Twenty-one genes were predicted in this region, among which the basic helix-loop-helix (bHLH) transcription factor TraesCS7A01G543300 was considered to be the most likely candidate gene for LERD1. The TraesCS7A01G543300 is highly homologous to the Arabidopsis ICE1 family proteins ICE/SCREAM, SCREAM2 and bHLH093, which control stomatal initiation and development. Two nucleotide variation sites were detected in the promoter region of TraesCS7A01G543300 between the two wheat cultivars. Gene expression assays indicated that TraesCS7A01G543300 was higher expressed in AK58 seedlings than that of ZM36. This research discovered a candidate gene related to wheat leaf rolling under drought stress, which may be helpful for understanding the leaf rolling mechanism and molecular breeding in wheat.
Genome-Wide Identification and Spatial Expression Analysis of Histone Modification Gene Families in the Rubber Dandelion Taraxacum kok-saghyz
Authors: Panara Francesco , Fasano Carlo , Lopez Loredana , Porceddu Andrea , Facella Paolo , Fantini Elio , Daddiego Loretta , Perrella Giorgio
Published: August 2022

Taraxacum kok-saghyz (Tks), also known as the Russian dandelion, is a recognized alternative source of natural rubber quite comparable, for quality and use, to the one obtained from the so-called rubber tree, Hevea brasiliensis. In addition to that, Tks roots produce several other compounds, including inulin, whose use in pharmaceutical and dietary products is quite extensive. Histone-modifying genes (HMGs) catalyze a series of post-translational modifications that affect chromatin organization and conformation, which, in turn, regulate many downstream processes, including gene expression. In this study, we present the first analysis of HMGs in Tks. Altogether, we identified 154 putative Tks homologs: 60 HMTs, 34 HDMs, 42 HATs, and 18 HDACs. Interestingly, whilst most of the classes showed similar numbers in other plant species, including M. truncatula and A. thaliana, HATs and HMT-PRMTs were indeed more abundant in Tks. Composition and structure analysis of Tks HMG proteins showed, for some classes, the presence of novel domains, suggesting a divergence from the canonical HMG model. The analysis of publicly available transcriptome datasets, combined with spatial expression of different developmental tissues, allowed us to identify several HMGs with a putative role in metabolite biosynthesis. Overall, our work describes HMG genomic organization and sets the premises for the functional characterization of epigenetic modifications in rubber-producing plants.
Effects of Nitrogen Forms on the Growth and Nitrogen Accumulation in Buchloe dactyloides Seedlings
Authors: Guo Lizhu , Meng Huizhen , Teng Ke , Fan Xifeng , Zhang Hui , Teng Wenjun , Yue Yuesen , Wu Juying
Published: August 2022

Buffalograss [Buchloe dactyloides (Nutt.) Engelm.] has become the most widely cultivated warm-season turfgrass in northern China because of its low-maintenance requirements. Nitrogen (N) can be applied to plants in a range of formulations. However, preference of nitrogen uptake and the effects of N form on plant growth and nitrogen accumulation has not been established in buffalograss. In this study, we evaluated the effects of different inorganic nitrogen forms (NO3−-N, NH4+-N, and NO3−-N: NH4+-N = 1:1) on growth and nitrogen accumulation in buffalograss seedlings. Results showed that supply of three N forms significantly increased buffalograss seedlings growth, biomass, and N contents of all plant organs compared with the seedlings receiving free nitrogen. Plants achieved better growth performance when they received nitrate as the sole N source, which stimulated stolon growth and increased the biomass of ramets, spacers, and aboveground and total plant biomass, and also allocated more biomass to ramets and more N to spacers. Meanwhile, those plants supplied with the treatment +NH4NO3 displayed a significantly greater N content in the ramet, 15N abundance, and 15N accumulation amount in all organs. These data suggest NO3−-N supplied either singly or in mixture increased vegetative propagation and thus facilitates buffalograss establishment. However, applications of ammonium caused detrimental effects on buffalograss seedlings growth, but +NO3− could alleviate NH4+-induced morphological disorders. Thus, recommendations to increase vegetative propagation and biomass accumulation in buffalograss seedlings should consider increasing NO3−-N in a fertility program and avoiding applications of nitrogen as NH4+-N.
Effects of m-Aramid/p-Aramid Blend Ratio on Tensile Strength due to UV Degradation for Firefighter Clothing Fabrics and Development of Predictive Equation for Tensile Strength
Authors: Wakatsuki Kaoru , Matsubara Minami , Watanabe Norimichi , Bao Limin , Morikawa Hideaki
Published: August 2022

This study focused on the m-Aramid/p-Aramid blend ratio of the fabrics, clarified the quantitative relationship between UV exposure and strength retention, and developed a mathematical model to calculate tensile strength from an arbitrary amount of UV exposure energy. The results of tensile strength tests before and after UV exposure showed that the decrease in tensile strength due to UV degradation depended on the combination of p-Aramid and m-Aramid blend percentages. Tensile strength for all blend ratios decreased exponentially with UV exposure energy and was within the range of results for fabrics with p-Aramid 100% and m-Aramid 100%. The retention fraction of tensile strength, which represents the tensile strength after UV exposure relative to the initial tensile strength, decreased exponentially with increasing the fraction of UV exposure energy for all fabrics used in this study. Fitting the retention fraction of tensile strength to the fraction of UV exposure energy, two groups of fabrics were classified based on m-Aramid blends of 40% or more and 60% or less. This model can predict the tensile strength of firefighter clothing fabrics that retain high mechanical strength when exposed to UV light and design the strength of firefighter clothing with consideration of degradation over time.
Skillful Control of Dispersion and 3D Network Structures: Advances in Functional Organic–Inorganic Nano-Hybrid Materials Prepared Using the Sol-Gel Method
Authors: Ikake Hiroki , Hara Shuta , Shimizu Shigeru
Published: August 2022

Organic–inorganic hybrid materials have become indispensable high-performance and highly functional materials. This is owing to the improved dispersion control in hybrid materials and emergence of functional ionic liquids. Harmonization of both these factors has enabled the utilization of functional 3D network structures and nanodispersions in composite materials. Polymeric materials endow materials with flexibility, toughness, and shape-memory properties, whereas inorganic materials provide materials with unique optical, electrical, and magnetic properties due to their nanosize. Organic–inorganic hybrid materials have evolved into novel materials that go beyond the composite rule. In this review, the historical development of hybrid materials prepared using the sol-gel method and the birth of ionic liquids have been summarized. In addition, the historical results leading to the development of functional 3D network structures and dispersion control have also been presented, as well as a review of the research on functional ionic liquids, which are of current interest. The authors also summarize the results of their research on functional ionic liquids. The design of new organic–inorganic hybrid materials has been discussed and the future prospects of new polymer composite materials provided.
Processing Effects on the Through-Plane Electrical Conductivities and Tensile Strengths of Microcellular-Injection-Molded Polypropylene Composites with Carbon Fibers
Authors: Chen Shia-Chung , Jien Ming-Yuan , Hsu Chi-Chuan , Hwang Shyh-Shin , Feng Ching-Te
Published: August 2022

Polymers reinforced with conducting fibers to achieve electrical conductivity have attracted remarkable attention in several engineering applications, and injection molding provides a cost-effective way for mass production. However, the electrical performance usually varies with the molding conditions. Moreover, high added content of conducting fibers usually results in molding difficulties. In this study, we propose using microcellular (MuCell) injection molding for polypropylene (PP)/carbon fiber (CF, 20, and 30 wt%) composites and hope that the MuCell injection molding process can improve both electrical and mechanical performance as compared with conventional injection molded (CIM) parts under the same CF content. Both molding techniques were also employed with and without gas counter pressure (GCP), and the overall fiber orientation, through-plane electrical conductivity (TPEC), and tensile strength (TS) of the composites were characterized. Based on the various processing technologies, the results can be described in four aspects: (1) Compared with CIM, microcellular foaming significantly influenced the fiber orientation, and the TPECs of the samples with 20 and 30 wt% CF were 18–78 and 5–8 times higher than those of the corresponding samples molded by CIM, respectively; (2) when GCP was employed in the CIM process, the TPEC of the samples with 20 and 30 wt% CF increased by 3 and 2 times, respectively. Similar results were obtained in the case of microcellular injection molding—the TPEC of the 20 and 30 wt% composites increased by 7–74 and 18–32 times, respectively; (3) although microcellular injection molding alone (i.e., without GCP) showed the greatest influence on the randomness of the fiber orientation and the TPEC, the TS of the samples was the lowest due to the uncontrollable foaming cell size and cell size uniformity; (4) in contrast, when GCP was employed in the microcellular foaming process, high TS was obtained, and the TPEC was significantly enhanced. The high foaming quality owing to the GCP implementation improved the randomness of fiber orientation, as well as the electrical and mechanical properties of the composites. Generally speaking, microcellular injection combined with gas counter pressure does provide a promising way to achieve high electrical and mechanical performance for carbon-fiber-added polypropylene composites.
Banana Waste-to-Energy Valorization by Microbial Fuel Cell Coupled with Anaerobic Digestion
Authors: Rincón-Catalán Néstor Isidro , Cruz-Salomón Abumalé , Sebastian P.J. , Pérez-Fabiel Sergio , Hernández-Cruz Maritza del Carmen , Sánchez-Albores Rocío Magdalena , Hernández-Méndez Jesús Mauricio Ernesto , Domínguez-Espinosa María Emperatriz , Esquinca-Avilés Héctor Armando , Ríos-Valdovinos Edna Iris , Nájera-Aguilar Hugo Alejandro
Published: August 2022

Banana is the most cultivated fruit plant in the world. It is produced in Latin America, Asia and Africa. India and China are the world’s largest banana producers, with almost 41% of the world’s production. This fruit reaches a total world production of 158.3 million tons per year. However, during their production cycle, the banana agroindustry produces large volumes of solid waste derived from overripe fruit. It contributes between 8–20 percent of the waste (around 100 kg of banana waste for every ton of banana produced). Therefore, the use of overripe banana waste represents a huge opportunity for bioenergy production. This work demonstrates that banana waste can be further used for power generation using a microbial fuel cell (MFC) coupled with anaerobic digestion (AD). First, the maximum methane production (MMP), methane production rate (MPR) and biochemical methane potential (BMP) were measured using an anaerobic batch bioreactor for 64 days of monitoring. Finally, the digestate generated from AD was used in the MFC to determine the polarization curve, maximum voltage, maximum power density (MPD), resistance and current. As a result, the AD generated an MMP of 320.3 mL, BMP of 373.3 mLCH4/gVS and MPR of 18.6 mLCH4/Lb⋅day. The MFC generated 286 mV (maximum voltage), 41.3 mW/m2 (MPD), 580.99 Ω (resistance) and 0.0002867 A (current). Both processes together produced a total bioenergy of 13.38 kJ/gVS. This coupled system showed a suitable and promising use of banana waste for ecofriendly bioenergy generation. Therefore, this feedstock could be taken advantage of for generating sustainable processes and developing a circular economy in the banana agroindustry.
New Directions in Modeling and Computational Methods for Complex Mechanical Dynamical Systems
Authors: Udwadia Firdaus E. , Mogharabin Nami
Published: August 2022

This paper presents a new conceptualization of complex nonlinear mechanical systems and develops new and novel computational methods for determining their response to given applied forces and torques. The new conceptualization uses the idea of including particles of zero mass to describe the dynamics of such systems. This leads to simplifications in the development of their equations of motion and engenders a straightforward new computational approach to simulate their behavior. The purpose of the paper is to develop a new analytical and computational methodology to handle complex systems and to illustrate it through the study of an old unsolved problem in classical mechanics, that of a non-uniform rigid spherical shell rolling, without slipping, under gravity on an arbitrary dimpled bowl-shaped rigid surface. The new conceptualization provides the explicit equations of motion for the system, the analytical determination of the reaction forces supplied by the surface, and a straightforward computational approach to simulate the dynamics. Detailed analytical and numerical results are provided. The computations illustrate the complexity of the dynamical behavior of the system and its high sensitivity to the initial orientation of the shell and to the presence of any initial angular velocity normal to the surface.
Remediation of Aqueous Phosphate Agricultural Runoff Using Slag and Al/Mg Modified Biochar
Authors: Crisler Glenn B. , Hernandez Cintly Guzman , Orr Andre , Davis Roger , Schauwecker Timothy , Johnson J. Casey , Sparks Darrell , Brown Ashli , Wilding Kelcie , Navarathna Chanaka , Mlsna Todd
Published: August 2022

Slag and Al/Mg oxide modified Douglas fir biochar (AMOB) were compared for their phosphate adsorbing abilities for use individually or in combination for simulated agriculture run-off remediation in wetlands. Aqueous batch and column sorption experiments were performed for both low-cost materials. AMOB was prepared in bulk using a novel green method. Material analyses included XRD, elemental analysis, SEM, EDX, and BET. Biochar and slag have different phosphate removal mechanisms. In short residence times (≤2 h), adsorption phenomena dominate for both adsorbents. Surface area likely plays a role in adsorption performance; slag was measured to be 4.1 m2/g while biochar’s surface area was 364.1 m2/g. In longer residence times (>2 h), the slow leaching of metals (Ca, Al, and Mg) from slag continue to remove phosphate through the precipitation of metal phosphates. In 24 h, slag removed more free phosphate from the solution than AMOB. Preliminary fixed bed column adsorption of slag or AMOB alone and in tandem was performed adopting a scaled-up model that can be used to remediate agricultural runoff with high phosphate content. Additionally, a desorption study was performed to analyze the efficiency of material regeneration. While AMOB does not release any adsorbed phosphates, slag slowly releases 5.7% adsorbed phosphate over seven days.
Quality Prediction Model of KICA-JITL-LWPLS Based on Wavelet Kernel Function
Authors: Sun Liangliang , Huang Yiren , Yang Mingyi
Published: August 2022

To obtain quality variables that cannot be measured in real time during the production process but reflect information on the quality of the final product, the batch production process has the characteristics of a strong time-varying nature, non-Gaussian data distribution and high nonlinearity. A locally weighted partial least squares regression quality prediction model (KICA-JITL-LWPLS), based on wavelet kernel function independent meta-analysis with immediate learning, is proposed. The model first measures the similarity between the normalized input data and the historical data and assigns the input data to the group of historical data with high similarity to it, based on the posterior probability of the Bayesian classifier; subsequently, wavelet kernel functions are selected and kernel learning methods are introduced into the independent meta-analysis algorithm. An independent meta-analysis, based on the wavelet kernel function, is performed on the classified input data to obtain probabilistically significant independent sets of variables. Finally, a real-time learning-based LWPLS regression analysis is performed on this variable set to construct a local prediction model for the current sample by calculating the similarity between the local input data. The local predictions from the PLS output are fused with the posterior probability output from the Bayesian classifier to produce the final prediction. The method was used to predict the product concentration and bacteriophage concentration during penicillin fermentation through a simulation platform. The prediction results were basically consistent with the real values, proving that the proposed KICA-JITL-LWPLS quality prediction model, based on wavelet kernel functions, has reliable prediction results.
Calibration of Physical Characteristic Parameters of Granular Fungal Fertilizer Based on Discrete Element Method
Authors: Yu Siyao , Bu Haoran , Dong Wancheng , Jiang Zhen , Zhang Lixin , Xia Yuanqing
Published: August 2022

Based on a combination of physical and simulation experiments, a set of basic physical characteristic parameters and contact parameters suitable for granular fungal fertilizers were calibrated, which up to now have received little attention. The physical morphological characteristic parameters (overall dimension, 1000 grain weight, density, and moisture content), mechanical characteristic parameters (Poisson’s ratio, elastic modulus, and shear modulus), and contact parameters (static friction coefficient, rolling friction coefficient and recovery coefficient) of particles were measured through physical experiments. The parameters were screened for significance using the Plackett–Burman test. The results showed that the impact recovery coefficient, static friction coefficient, and rolling friction coefficient for the granular fungal fertilizer particles had a significant effect on the repose angle. The best range for the three parameters was determined using the steepest climbing test. The second-order regression model was optimized using the Box–Behnken design test. The relative error between the simulated repose angle (21.74°) and the physical test repose angle (21.84°) was small (0.50%). These optimal parameters obtained by calibration can provide a basis for the simulation analysis and parameter optimization of variable-rate fertilization systems.
High-Efficient Anionic Dyes Removal from Water by Cationic Polymer Brush Functionalized Magnetic Mesoporous Silica Nanoparticles
Authors: Beagan Abeer , Alshammari Riyadh , Alotaibi Lamya , Albarrak Hadeel , Alotaibi Khalid , Alswieleh Abdullah
Published: August 2022

High efficiency removal of methyl orange (MO) and bromothymol blue (BT) dyes from contaminated water has been reported using magnetic mesoporous nanoparticles modified with cationic polymer brush (poly(2-methacryloyloxy)ethyl] trimethylammonium chloride solution) (Fe3O4-MSNs-PMETAC). Atom transfer radical polymerization (ATRP) was utilized to grow the polymer chains on the magnetic mesoporous silica nanoparticles. The chemical surface modifications were confirmed using IR, TGA, SEM and TEM. The results show that the obtained Fe3O4-MSNs-PMETAC materials were nearly spherical in shape with approximately 30 nm magnetic core, and silica shell thicknesses ranged from 135 to 250 nm. The adsorption performance of the material was found to be unaffected by the pH (3-9) of the media, with a removal efficiency of 100% for both dyes. The adsorption of BT and MO on the surface of Fe3O4-MSNs-PMETAC was found to follow Freundlich and Langmuir models, respectively. Since the synthesized nanocomposite materials exhibit an enhanced properties such as large maximum adsorption capacity, rapid synthesis process, and easy separation from solution, it could be an effective sorbent for the removal of other pollutants such as potentially toxic anionic elements (e.g., arsenate and chromate ions) from water and wastewater.
Relief Mechanism of Segmented Hole Reaming and Stress Distribution Characteristics of Drilling Holes in Deep Coal Mine
Authors: Zhang Lei , Huang Peng , Liu Sijia , He Gang , Li Bing , Cao Yuanwei
Published: August 2022

As the mining depth increases, in underground tunnels, caverns or pillar goaf, a rock burst is one of the important accidents that threaten mine safety. Drilling pressure relief becomes one of the main means of preventing rock bursts, which affect the mechanical properties and stability of the coal and rock in underground excavations. However, the surrounding rock of the roadway or the coal body is usually broken, and the pressure relief of the large-diameter borehole will affect its support. A segmented hole-reaming technology is proposed and applied in a coal mine in China. A pressure-relief mechanic model of segmented hole reaming was built. The coal sample had an elastic modulus of 0.35 GPa, the UCS and UTS were 17.4 MPa and 1.41 MPa, the Poisson ratio was 0.27, the cohesive force was 2.81 MPa, and the friction was 23.7°. The pressure relief range of the boreholes with different diameters, horizontal in situ stress coefficients, cohesive forces, and friction angles were analyzed. When the drill hole was increased from 120 mm to 200 mm, the pressure relief range was increased by 57.1%. The stress distribution of the staged reaming and pressure-relief drilling was also obtained. In the vertical direction, the vertical stress of the borehole first decreased and then was restored to the original rock stress area, and in the horizontal direction, it first increased and then was restored to the original rock stress area. A CMS1-6200 segmented drilling rig was used to construct the pressure relief hole. The weight of the drilled pulverized coal was monitored at different depths. The results showed that the amount of pulverized coal in all of the drilled holes was less than 3 kg/m, indicating that the effect of reducing the pressure relief is obvious. The study is of great significance to research the pressure relief range, mechanical characteristics and stress distribution of segmented hole reaming; it also provides insight into the rock burst prevention and the design of drilling in the mine site.
Energy and Exergy Analysis of a Modified Absorption Heat Pump (MAHP) to Produce Electrical Energy and Revaluated Heat
Authors: Hernández-Magallanes Javier Alejandro , Domínguez-Inzunza L. A. , Lugo-Loredo Shadai , Sanal K. C. , Cerdán-Pasarán Andrea , Tututi-Avila Salvador , Morales L. I.
Published: August 2022

The novel modified absorption heat pump (MAHP) with the H2O-LiBr working mixture for cogeneration applications is introduced. The MAHP can simultaneously produce electric energy and heat revaluation. The proposed system has the particularity that it can be powered by alternative thermal sources (such as solar energy, biomass, geothermal) or industrial waste heat, thus promoting the production and efficient use of clean energy. The effects of pressure ratio (RP), source or supply temperature (TGH), and the energy revaluation gradient (GTL) are analyzed. The critical parameters of the proposed system are evaluated, including thermal efficiency (ηTh), exergetic efficiency (ηEx), revaluated heat (Q˙A), as well as net power produced (˙Wnet). For the MAHP analysis, RP and TGH operating ranges were chosen at 1.1–15.0 and 100–160 °C, respectively. The results show that ηEx of 87% can be obtained, having the maximum performance in TGH of 120 °C, RP of 1.1, and GTL of 35 °C. The ηTh varies between 51% and 55%, having a maximum GTL of 45 °C. On the other hand, ˙Wnet achieves values between 260 and 582 kW, depending on the defined operating conditions.
Metabolomics in Team-Sport Athletes: Current Knowledge, Challenges, and Future Perspectives
Authors: Bongiovanni Tindaro , Lacome Mathieu , Fanos Vassilios , Martera Giulia , Cione Erika , Cannataro Roberto
Published: August 2022

Metabolomics is a promising tool for studying exercise physiology and exercise-associated metabolism. It has recently been defined with the term “sportomics” due to metabolomics’ capability to characterize several metabolites in several biological samples simultaneously. This narrative review on exercise metabolomics provides an initial and brief overview of the different metabolomics technologies, sample collection, and further processing steps employed for sport. It also discusses the data analysis and its biological interpretation. Thus, we do not cover sample collection, preparation, and analysis paragraphs in detail here but outline a general outlook to help the reader to understand the metabolomics studies conducted in team-sports athletes, alongside endeavoring to recognize existing or emergent trends and deal with upcoming directions in the field of exercise metabolomics in a team-sports setting.