bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolution of Root Morphology in Table Beet: Historical and Iconographic
Authors: Goldman Irwin L. , Janick Jules
Published: August 2021

The Beta vulgaris complex includes sugar beet, mangel wurzel, Swiss chard, fodder beet, and table beet. Mangel wurzel and fodder beet are considered to be the same general crop type, with the former possessing lower dry matter content (<13%) than the latter. Mangel is likely derived from crosses between table beet and chard, while fodder beet may have a more recent origin, arising from crosses between mangel and sugarbeet. The table beet was derived from the wild sea beet, B. vulgaris (L.) subsp. maritima (L.) Arcang, with small non-spherical roots. Table beet is presently a popular vegetable cultivated for its pigmented roots, typically red but also yellow and other colors. Wild forms were consumed in antiquity mainly for their leaves with roots used medicinally. Beet is referred to in the Septuagint, a Greek translation of the first five books of the Hebrew bible, made in Ptolomeic Egypt in the third century BCE. A beet identified as Beta maritima is included in De Material Medicus of Pedanius Dioscorides written in the first century CE, and the first illustrated version of 512, known as the Juliana Anicia Codex, includes an image with non-spherical root. Beet is mentioned in several tractates of the Talmud, a sixth century collection of history and civil law written in Babylonia. Beta maritima possesses supernumerary root cambia, which facilitated selection of swollen rooted forms. The first colored illustration of swollen rooted table beet, B. vulgaris, can be found in the 1515–1517 frescos of Raphael Sanzio and Giovanni Martina da Udine in the Villa Farnesina in Rome. Swollen roots in Roman beet are illustrated and described in the 1587 French herbal Historia Generalis Plantarum of Jacques Dalechamps. Conically shaped beet roots are found in the market painting of Franz Snijders in the 17th century. Various spherical forms of beet root are found in the work of American painter James Peale in 1826. A complete array of beet root types is found in the Benary catalog of 1876. Modern, spherical beet roots were depicted in 1936 by the Russian painter Zinaida Serebriankov, 1936. Artistic and historical representations of table beet suggest that swollen rooted forms have existed during the past five centuries, but conically shaped roots were gradually replaced by spherically shaped roots during this period.
Genome-Wide Identification of the ARF Gene Family and ARF3 Target Genes Regulating Ovary Initiation in Hazel via ChIP Sequencing
Authors: Wei Heng , Cheng Yunqing , Sun Ying , Zhang Xingzheng , He Hongli , Liu Jianfeng
Published: August 2021

Hazel (Corylus spp.) is an economically important nut species with a unique biological characteristic of ovary differentiation and development initiating from the ovary primordium after pollination. Auxin participates in ovary initiation and has an essential impact on hazel fruit yield and quality. The regulation of auxin in ovary development is thought to be related to auxin response factors (ARFs); however, its detailed regulatory mechanism remains unclear. The spatiotemporal expression pattern of C. heterophylla ARF3 (ChARF3) was accessed via ARF gene family member identification and expression abundance analysis as well as immunohistochemistry. ChARF3 target genes were identified via chromatin immunoprecipitation followed by next-generation sequencing (ChIP-Seq). In total, 14 ChARF members containing at least B3 and Auxin_resp domains were found to be distributed on 9 of 11 chromosomes, and the protein molecular weights were predicted to range from 70.93–139.22 kD. Among eight differentially expressed ChARFs, ChARF3 showed the most significant differences over four ovary developmental stages. Immunohistochemical analysis revealed that ChARF3 was expressed in the ovary primordium and funiculus, integument, endosperm, radicle, and cotyledon indicating its potential regulatory roles in ovary differentiation and development. In total, 3,167 ChARF3 target genes were identified through ChIP-Seq in four ovary developmental stages and were significantly enriched in the biosynthesis of secondary metabolites (ko01110), phenylpropanoid biosynthesis (ko00940), and phytohormone signal transduction (ko04075). ChARF3 was hypothesized to be involved in the regulation of auxin-induced genes and the transcription factors MADS, AP2/ERF, TCP, FT, and LFY. These results suggest that ChARF3 may regulate ovary initiation and ovule development by mediating genes related to auxin biosynthesis and transport, cell division and proliferation, and flower and fruit development. This study provides new insights into the molecular mechanism of hazel yield formation.
Recall Accuracy in Children: Age vs. Conceptual Thinking
Authors: Murnikov Valeri , Kask Kristjan
Published: August 2021

The aim of this study was to replicate a previous experiment using a different stimulus event. The present study examined the relationship between age, development of conceptual thinking, and responses to free recall, suggestive and specific option-posing questions in children and adults. Sixty-three children (aged 7–14) and 30 adults took part in an experiment in which they first participated in a live staged event, then, a week later, were interviewed about the event and tested using the Word Meaning Structure Test. Age and level of conceptual thinking were positively correlated in children. Compared to age, conceptual thinking ability better predicted children's accurate free recall and inaccurate responses to specific option-posing questions, but not inaccurate responses to suggestive questions.
Trust Miscalibration Is Sometimes Necessary: An Empirical Study and a Computational Model
Authors: Collins Michael G. , Juvina Ion
Published: August 2021

The literature on trust seems to have reached a consensus that appropriately calibrated trust in humans or machines is highly desirable; miscalibrated (i.e., over- or under-) trust has been thought to only have negative consequences (i.e., over-reliance or under-utilization). While not invalidating the general idea of trust calibration, a published computational cognitive model of trust in strategic interaction predicts that some local and temporary violations of the trust calibration principle are critical for sustained success in strategic situations characterized by interdependence and uncertainty (e.g., trust game, prisoner’s dilemma, and Hawk-dove). This paper presents empirical and computational modeling work aimed at testing the predictions of under- and over-trust in an extension of the trust game, the multi-arm trust game, that captures some important characteristics of real-world interpersonal and human-machine interactions, such as the ability to choose when and with whom to interact among multiple agents. As predicted by our previous model, we found that, under conditions of increased trust necessity, participants actively reconstructed their trust-investment portfolios by discounting their trust in their previously trusted counterparts and attempting to develop trust with the counterparts that they previously distrusted. We argue that studying these exceptions of the principle of trust calibration might be critical for understanding long-term trust calibration in dynamic environments.
Social Isolation, Loneliness and Well-Being: The Impact of WeChat Use Intensity During the COVID-19 Pandemic in China
Authors: Li Jianfeng , Zhou Luyang , Van Der Heijden Beatrice , Li Shengxiao , Tao Hong , Guo Zhiwen
Published: August 2021

This study is aimed to examine the impact of WeChat use intensity on social isolation, loneliness, and well-being during the lockdown period of the COVID-19 pandemic. Drawing on the regulatory loop model of loneliness, the notions of Internet Paradox, the Time Displacement hypothesis and previous literature on WeChat use intensity, we propose that lockdown loneliness (partially) mediates the relationship between lockdown WeChat use intensity and well-being (i.e., lockdown stress and lockdown life satisfaction). Moreover, we assume that lockdown WeChat use intensity moderates the relationship between lockdown social isolation and well-being (i.e., lockdown stress and lockdown life satisfaction) in both a direct and in an indirect way, that is through lockdown loneliness. The results from our Structural Equation Modeling analyses, using a sample of 1,805 Chinese respondents, indicate that all of our research hypotheses are confirmed. From this empirical work, it becomes clear that online social interactions, which are believed by many people to be able to compensate for the lack of offline social interactions during the COVID-19 lockdown period, in fact are endangering their mental health and life satisfaction instead. This article concludes with theoretical and practical implications of our study, followed by its limitations and recommendations for future research.
Pre-service Teachers Learning to Teach English as a Foreign Language to Preschool Learners in Macau: A Longitudinal Study
Authors: Reynolds Barry Lee , Liu Sylvia , Ha Xuan Van , Zhang Xiaofang , Ding Chen
Published: August 2021

Language teacher beliefs have received increasing research attention for the past few decades. However, little is known about the beliefs of pre-service teachers in the pre-primary English as a foreign language (EFL) education context. This qualitative case study extends this line of inquiry by investigating the trajectory of student teachers' beliefs about teaching English to pre-primary learners in Macau within a teacher education course. The participants included 60 pre-service teachers taking anEnglish Language Activitiescourse in their third year of a 4-year Bachelor of pre-primary education program. The data comprised written reflections collected at three points in time during the 16-week course: at the beginning of the course, mid-way through the course, and at the end of the course. The findings showed five broad themes, constituted from 15 subthemes, regarding (1) learners and learning, (2) teaching, (3) subject, (4) self, and (5) learning to teach. The major themes have been documented in the literature, but several subthemes were identified for the first time in the context of pre-primary EFL teacher education. More importantly, the findings revealed that some of the subthemes were newly shaped and several subthemes were reshaped as a consequence of taking the course. The findings were interpreted in relation to the content of the course, the experiential learning opportunities, the pre-service teachers' prior experiences of language learning and teaching, and the local language teaching and learning context. Implications for pre-service teacher education programs are discussed.
D-Serine: A Cross Species Review of Safety
Authors: Meftah Amir , Hasegawa Hiroshi , Kantrowitz Joshua T.
Published: August 2021

Background:D-Serine, a direct, full agonist at theD-serine/glycine modulatory site of the N-methyl-D-aspartate-type glutamate receptors (NMDAR), has been assessed as a treatment for multiple psychiatric and neurological conditions. Based on studies in rats, concerns of nephrotoxicity have limitedD-serine research in humans, particularly using high doses. A review ofD-serine's safety is timely and pertinent, asD-serine remains under active study for schizophrenia, both directly (R61 MH116093) and indirectly throughD-amino acid oxidase (DAAO) inhibitors. The principal focus is on nephrotoxicity, but safety in other physiologic and pathophysiologic systems are also reviewed.

Methods:Using the search terms “D-serine,” “D-serine and schizophrenia,” “D-serine and safety,” “D-serine and nephrotoxicity” in PubMed, we conducted a systematic review onD-serine safety.D-serine physiology, dose-response and efficacy in clinical studies anddAAO inhibitor safety is also discussed.

Results:WhenD-serine doses >500 mg/kg are used in rats, nephrotoxicity, manifesting as an acute tubular necrosis syndrome, seen within hours of administration is highly common, if not universal. In other species, however,D-serine induced nephrotoxicity has not been reported, even in other rodent species such as mice and rabbits. Even in rats,D--serine related toxicity is dose dependent and reversible; and does not appear to be present in rats at doses producing an acute Cmax of <2,000 nmol/mL. For comparison, the Cmax ofD-serine 120 mg/kg, the highest dose tested in humans, is ~500 nmol/mL in acute dosing. Across all published human studies, only one subject has been reported to have abnormal renal values related toD-serine treatment. This abnormality did not clearly map on to the acute tubular necrosis syndrome seen in rats, and fully resolved within a few days of stopping treatment.DAAO inhibitors may be nephroprotective.D-Serine may have a physiologic role in metabolic, extra-pyramidal, cardiac and other systems, but no other clinically significant safety concerns are revealed in the literature.

Conclusions:Even before considering human to rat differences in renal physiology, using current FDA guided monitoring paradigms,D-serine appears safe at currently studied maximal doses, with potential safety in combination withDAAO inhibitors.
Early Surveillance and Public Health Emergency Responses Between Novel Coronavirus Disease 2019 and Avian Influenza in China: A Case-Comparison Study
Authors: Zhang Tiantian , Wang Qian , Wang Ying , Bai Ge , Dai Ruiming , Luo Li
Published: August 2021

Background: Since the novel coronavirus disease (COVID-19) has been a worldwide pandemic, the early surveillance and public health emergency disposal are considered crucial to curb this emerging infectious disease. However, studies of COVID-19 on this topic in China are relatively few.

Methods: A case-comparison study was conducted using a set of six key time nodes to form a reference framework for evaluating early surveillance and public health emergency disposal between H7N9 avian influenza (2013) in Shanghai and COVID-19 in Wuhan, China.

Findings: A report to the local Center for Disease Control and Prevention, China, for the first hospitalized patient was sent after 6 and 20 days for H7N9 avian influenza and COVID-19, respectively. In contrast, the pathogen was identified faster in the case of COVID-19 than in the case of H7N9 avian influenza (12 vs. 31 days). The government response to COVID-19 was 10 days later than that to avian influenza. The entire process of early surveillance and public health emergency disposal lasted 5 days longer in COVID-19 than in H7N9 avian influenza (46 vs. 41 days).

Conclusions: The identification of the unknown pathogen improved in China between the outbreaks of avian influenza and COVID-19. The longer emergency disposal period in the case of COVID-19 could be attributed to the government's slower response to the epidemic. Improving public health emergency management could lessen the adverse social effects of emerging infectious diseases and public health crisis in the future.
Aging and CMV Infection Affect Pre-existing SARS-CoV-2-Reactive CD8+ T Cells in Unexposed Individuals
Authors: Jo Norihide , Zhang Rui , Ueno Hideki , Yamamoto Takuya , Weiskopf Daniela , Nagao Miki , Yamanaka Shinya , Hamazaki Yoko
Published: August 2021

Age is a major risk factor for COVID-19 severity, and T cells play a central role in anti-SARS-CoV-2 immunity. Because SARS-CoV-2-cross-reactive T cells have been detected in unexposed individuals, we investigated the age-related differences in pre-existing SARS-CoV-2-reactive T cells. SARS-CoV-2-reactive CD4+ T cells from young and elderly individuals were mainly detected in the central memory fraction and exhibited similar functionalities and numbers. Naïve-phenotype SARS-CoV-2-reactive CD8+ T cell populations decreased markedly in the elderly, while those with terminally differentiated and senescent phenotypes increased. Furthermore, senescent SARS-CoV-2-reactive CD8+ T cell populations were higher in cytomegalovirus seropositive young individuals compared to seronegative ones. Our findings suggest that age-related differences in pre-existing SARS-CoV-2-reactive CD8+ T cells may explain the poor outcomes in elderly patients and that cytomegalovirus infection is a potential factor affecting CD8+ T cell immunity against SARS-CoV-2. Thus, this study provides insights for developing effective therapeutic and vaccination strategies for the elderly.
Simultaneous Aerosol and Ocean Properties From the PolCube CubeSat Polarimeter
Authors: Stamnes Snorre , Baize Rosemary , Bontempi Paula , Cairns Brian , Chemyakin Eduard , Choi Young-Jun , Chowdhary Jacek , Hu Yongxiang , Jeong Minsup , Kang Kyung-In , Kim Sungsoo S. , Liu Xu , Loughman Robert , MacDonnell Dave , McCormick M. Patrick , Moon Bongkon , Omar Ali , Roithmayr Carlos M. , Sim Chae Kyung , Sun Wenbo , van Diedenhoven Bastiaan , Videen Gorden , Wasilewski Andrzej
Published: August 2021

We quantify the performance of aerosol and ocean remote sensing products from the PolCube instrument using a previously developed polarimeter retrieval algorithm based on optimal estimation. PolCube is a modified version of the PolCam lunar instrument on the Korea Pathfinder Lunar Orbiter that has been optimized for Earth-Science observations of aerosol, ocean, and thin cloud optical properties. The objective of the PolCube instrument is to retrieve detailed fine-mode (pollution and smoke) and coarse-mode (sea-salt and dust) aerosol properties over the ocean for a range of light to heavy aerosol loadings using its polarimetric-imaging capability at multiple angles and wavelengths from 410−865 nm. An additional objective is to discriminate aerosols from thin clouds. PolCube’s retrieval performance of aerosol optical and microphysical properties and ocean products is quantitatively assessed. We estimate that PolCube can retrieve total aerosol optical depth at 555 nm (AOD555) within ±0.068, fine-mode AOD555 within ±0.078, and fine-mode single-scattering albedo within ±0.036, where all uncertainties are expressed as one standard deviation (1σ). PolCube’s accurate and high-resolution aerosol-retrieval products will provide unique spatial and temporal coverage of the Earth that can be used synergistically with other instruments, such as the Geostationary Environmental Monitoring Spectrometer to improve air-quality forecasting.
Using Ultrasonic Haptics Within an Immersive Spider Exposure Environment to Provide a Multi-Sensorial Experience
Authors: Brice Daniel , Gibson Zara , McGuinness Fintan , Rafferty Karen
Published: August 2021

A proof of concept virtual reality system is presented that integrates ultrasonic feedback sensations to provide a demonstrative virtual reality exposure therapy environment containing multiple scenarios with virtual spiders. This system and environment were utilised to conduct a study containing 35 participants with the goal of investigating the effect the environment could have on their level of anxiety. This level of anxiety was measured in three different forms: changes in frontal asymmetry analysis of EEG data, changes in skin conductance levels and subjective units of distress. The Fear of Spiders Questionnaire was used to determine which participants in the study reported to be moderately afraid of spiders. For these participants all three measurement forms for anxiety showed statistically significant increases in a comparison between baseline and scenarios with the virtual spiders. A statistically significant correlation between scores on the Fear of Spiders Questionnaire and changes in anxiety shows the system to have had a greater effect on the anxiety levels of those who were more afraid of spiders, than those who were not. There was also a statistically significant correlation discovered between immersion and increase in anxiety, highlighting the significance of immersion in future virtual reality exposure therapy applications.
Model-Free-Based Single-Dimension Fuzzy SMC Design for Underactuated Quadrotor UAV
Authors: Abro Ghulam E Mustafa , Zulkifli Saiful Azrin B. M. , Asirvadam Vijanth Sagayan , Ali Zain Anwar
Published: August 2021

The underactuated quadrotor unmanned aerial vehicle (UAV) is one of the nonlinear systems that have few actuators as compared to the degree of freedom (DOF); thus, it is a strenuous task to stabilize its attitude and positions. Moreover, an induction of unmodelled dynamic factors and uncertainties make it more difficult to control its maneuverability. In this paper, a model-free based single-dimension fuzzy sliding mode control (MFSDF-SMC) is proposed to control the attitude and positions of underactuated quadrotor UAV. The paper discusses the kinematic and dynamic models with unmodelled dynamic factors and unknown external disturbances. These unmodelled factors and disturbances may lead the quadrotor towards failure in tracking specific trajectory and may also generate some serious transient and steady-state issues. Furthermore, to avoid the problem of gimbal lock, the model is amalgamated with hyperbolic function to resolve the singularity issues dully developed due to Newton Euler’s dynamic modeling. The simulation results performed for MFSDF-SMC using MATLAB software R2020a are compared with conventional sliding mode control, fuzzy-based sliding control and single-dimension fuzzy-based sliding mode control without a model-free approach. The design and implementation of the model-free single dimension-based fuzzy sliding mode control (MFSDF-SMC) with an updated Lyapunov stability theorem is presented in this work. It is observed that MFSDF-SMC produces robust trajectory performance therefore, and the manuscript suggests the experimental setup to test the proposed algorithm in a noisy environment keeping the same conditions. The verification of the equipment used and its effective demonstration is also available for the reader within the manuscript.
A Parametric Study on the Aeroelasticity of Flared Hinge Folding Wingtips
Authors: Ajaj Rafic M. , Saavedra Flores Erick I. , Amoozgar Mohammadreza , Cooper Jonathan E.
Published: August 2021

This paper presents a parametric study on the aeroelasticity of cantilever wings equipped with Flared Hinge Folding Wingtips (FHFWTs). The finite element method is utilized to develop a computational, low-fidelity aeroelastic model. The wing structure is modelled using Euler–Bernoulli beam elements, and unsteady Theodorsen’s aerodynamic strip Theory is used for aerodynamic load predictions. The PK method is used to estimate the aeroelastic boundaries. The model is validated using three rectangular, cantilever wings whose properties are available in literature. Then, a rectangular, cantilever wing is used to study the effect of folding wingtips on the aeroelastic response and stability boundaries. Two scenarios are considered for the aeroelastic analysis. In the first scenario, the baseline, rectangular wing is split into inboard and outboard segments connected by a flared hinge that allows the outboard segment to fold. In the second scenario, a folding wingtip is added to the baseline wing. For both scenarios, the influence of fold angle, hinge-line angle (flare angle), hinge stiffness, tip mass and geometry are assessed. In addition, the load alleviation capability of FHFWT is evaluated when the wing encounters discrete (1-cosine) gusts. Finally, the hinge is assumed to exhibit cubic nonlinear behavior in torsion, and the effect of nonlinearity on the aeroelastic response is assessed and analyzed for three different cases.
Overview of the Flight Dynamics Subsystem for Korea Pathfinder Lunar Orbiter Mission
Authors: Song Young-Joo , Kim Young-Rok , Bae Jonghee , Park Jae-ik , Hong Seungbum , Lee Donghun , Kim Dae-Kwan
Published: August 2021

Korea’s first lunar mission, the Korea Pathfinder Lunar Orbiter (KPLO), aims to launch in mid-2022 via the Space-X Falcon-9 launch vehicle. For the successful flight operation of KPLO, the Korea Aerospace Research Institute (KARI) has designed and developed the Flight Dynamics Subsystem (FDS). FDS is one of the subsystems in the KPLO Deep-Space Ground System (KDGS), which is responsible for the overall flight dynamics-related operation. FDS is currently successfully implemented and meets all of the requirements derived from the critical design phases. The current work addresses the design and implementation results for the KPLO FDS. Starting from overviews on KPLO payloads, bus systems, and mission trajectory characteristics, a review on KDGS is also treated briefly. Details on the design philosophy, unique characteristics, and functionalities of all six different modules nested inside the FDS with its Graphical User Interface (GUI) design are discussed. Moreover, efforts currently devoted to the flight operation preparation of the KPLO are summarized, including many collaborative works between KARI and the National Aeronautics and Space Administration (NASA) teams.
Growth Performance and Plasma Metabolites of Grazing Beef Cattle Backgrounded on Buffel or Buffel-Desmanthus Mixed Pastures
Authors: Mwangi Felista W. , Gardiner Christopher P. , Walker Glen , Hall Trevor J. , Malau-Aduli Bunmi S. , Kinobe Robert T. , Malau-Aduli Aduli E. O.
Published: August 2021

Dietary crude protein and dry matter digestibility are among the major factors limiting feed intake and weight gain of cattle grazing native and improved pastures in the subtropics of Northern Australia during the dry season. Incorporating a suitable legume into grasses improves pasture quality and cattle weight gain, but only a limited number of legume pastures can establish and persist in cracking clay soils. This study aimed to evaluate the effect of Desmanthus inclusion in buffel grass (Cenchrus ciliaris) pastures on the plasma metabolite profile and growth performance of grazing beef cattle during the dry season. We hypothesised that backgrounding steers on buffel grass-Desmanthus mixed pastures would elicit significant changes in plasma glucose, bilirubin, creatinine, non-esterified fatty acids and β-hydroxybutyrate, resulting in higher liveweight gains than in steers on buffel grass only pastures. Four hundred tropical composite steers were assigned to buffel grass only (n = 200) or buffel grass oversown with Desmanthus (11.5% initial sward dry matter) pastures (n = 200) and grazed for 147 days during the dry season. Desmanthus accounted for 6.2% sward dry matter at the end of grazing period. Plasma metabolites results showed that changes in β-hydroxybutyrate, creatinine, bilirubin, glucose and non-esterified fatty acids were within the expected normal range for all the steers, indicating that with or without Desmanthus inclusion in the diet of grazing steers, animal health status was not compromised. It was also evident that Desmanthus inclusion in buffel grass pastures had no impact on the plasma metabolite profile, liveweight and daily weight gain of grazing steers. Therefore, our tested hypothesis of higher changes in plasma metabolite profile and higher liveweight gains due to backgrounding on low-level buffel grass-Desmanthus mixed pastures does not hold.
Role of Two-Dimensional Speckle-Tracking Echocardiography in Early Detection of Left Ventricular Dysfunction in Dogs
Authors: Hamabe Lina , Mandour Ahmed S. , Shimada Kazumi , Uemura Akiko , Yilmaz Zeki , Nagaoka Kentaro , Tanaka Ryou
Published: August 2021

Two-dimensional speckle-tracking echocardiography (2D–STE) is an advanced echocardiographic technique based on deformation imaging that allows comprehensive evaluation of the myocardial function. Clinical application of 2D–STE holds great potential for its ability to provide valuable information on both global and regional myocardial function and to quantify cardiac rotation and synchronicity, which are not readily possible with the conventional echocardiography. It has gained growing importance over the past decade, especially in human medicine, and its application includes assessment of myocardial function, detection of subclinical myocardial dysfunction and serving as a prognostic indicator. This review illustrates the fundamental concepts of deformation analysis and gives an overview of the current understanding and its clinical application of this technique in veterinary medicine, with a focus on early detection of left ventricular (LV) dysfunction in dogs.
Effect of Drinking Water Distribution System Design on Antimicrobial Delivery to Pigs
Authors: Little Stephen , Woodward Andrew , Browning Glenn , Billman-Jacobe Helen
Published: August 2021

On many pig farms, growing pigs are mass-medicated for short periods with antimicrobial drugs through their drinking water for metaphylaxis and to treat clinical disease. We conducted a series of four prospective observational cohort studies of routine metaphylactic in-water antibiotic dosing events on a commercial pig farm, to assess the concentration of antimicrobial available to pigs throughout a building over time. Each dosing event was conducted by the farm manager with a differently designed looped water distribution system (WDS). We found that the antimicrobial concentration in water delivered to pigs at drinkers in each pen by a building’s WDS over time was profoundly influenced by the design of the WDS and the pigs’ water usage and drinking pattern, and that differences in the antimicrobial concentration in water over time at drinkers throughout a building could be eliminated through use of a circulator pump in a looped WDS. We also used a hydraulic WDS modelling tool to predict the antimicrobial concentration at drinkers over time during and after a dosing event. Our approach could be used to evaluate alternative in-water dosing regimens for pigs in a specific building in terms of their clinical efficacy and ability to suppress the emergence of antimicrobial resistance, and to determine the optimal regimen. The approach is applicable to all additives administered through drinking water for which the degree of efficacy is dependent on the dose administered.
The Toxic Effect of Water-Soluble Particulate Pollutants from Biomass Burning on Alveolar Lung Cells
Authors: Lima de Albuquerque Yuri , Berger Emmanuelle , Li Chunlin , Pardo Michal , George Christian , Rudich Yinon , Géloën Alain
Published: August 2021

In 2018, 3.8 million premature deaths were attributed to exposure to biomass burning nanoparticles from wood combustion. The objective of this study was to investigate and compare the toxic effect of wood-combustion-related biomass burning nanoparticles from three different combustion stages (i.e., flaming, smoldering, and pyrolysis) on alveolar lung cells, by studying cell proliferation, and structural and behavioral parameters. A549 lung epithelial cells were treated with 31, 62, 125, 250, and 500 µg/mL of water-soluble particulate pollutants from wood burning, and measured by means of real-time cell analysis, cell imaging, and phase imaging microscopy. At low concentrations (31 and 62 µg/mL), all three types of wood burning samples exhibited no toxicity. At 125 µg/mL, they caused decreased cell proliferation compared to the control. Exposure to higher concentrations (250 and 500 µg/mL) killed the cells. Cell physical parameters (area, optical volume, eccentricity, perimeter, and optical thickness) and behavioral parameters (migration, motility, and motility speed) did not change in response to exposure to wood burning materials up to a concentration of 125 µg/mL. Exposure to higher concentrations (250 and 500 µg/mL) changed cell perimeter, optical thickness for smoldering and flaming particles, and led to decreased migration, motility, and motility speed of cells. In conclusion, all three of the combustion water-soluble organic pollutants were identified as equally toxic by real-time cell analysis (RTCA) results. The parameters describing cell structure suggest that pyrolysis particles were slightly less toxic than others.
Association between Novel Object Recognition/Spontaneous Alternation Behavior and Emission of Ultrasonic Vocalizations in Rats: Possible Relevance to the Study of Memory
Authors: Costa Giulia , Serra Marcello , Simola Nicola
Published: August 2021

Rats emit ultrasonic vocalizations (USVs) in situations with emotional valence, and USVs have also been proposed as a marker for memories conditioned to those situations. This study investigated whether USV emissions can predict and/or be associated with the behavior of rats in tests that evaluate unconditioned memory. To this end, rats were subjected to “tickling”, a procedure of heterospecific play that has emotional valence and elicits the emission of USVs, and afterwards evaluated in the novel object recognition test (NOR) and in the single trial continuous spontaneous alternation behavior (SAB) test in a Y maze. The number of 22-kHz USVs (aversive) and 50-kHz USVs (appetitive) emitted in response to tickling and during NOR and SAB tests were scored, and the correlations among them and with rats’ behavior evaluated. Rats emitted 50-kHz USVs, but not 22-kHz USVs, during the NOR and SAB tests, and such calling behavior was not linked with the behavioral readouts indicative of memory function in either test. However, rats that prevalently emitted 22-kHz USVs in response to tickling displayed an impaired NOR performance. These findings suggest that measuring the emission of USVs could be of interest in studies of unconditioned memory, at least with regard to 22-kHz USVs.
Atrial Fibrillation after Lung Cancer Surgery: Prediction, Prevention and Anticoagulation Management
Authors: Semeraro Gennaro Carmine , Meroni Carlo Ambrogio , Cipolla Carlo Maria , Cardinale Daniela Maria
Published: August 2021

Atrial fibrillation (AF) is a common complication of the early postoperative period of various types of surgery, including that for lung cancer. Although induced by the homeostatic alterations related to surgery, there is evidence that it is not a mere stand-alone transitory event, but it represents a relevant complication of surgery, bearing considerable prognostic consequences. Different methods have therefore been explored to predict the occurrence of postoperative atrial fibrillation (POAF) and prevent it. In particular, the age among clinical factors, and N-terminal prohormone of brain natriuretic peptide (NT-proBNP), as a marker, have proven to be good predictors, and the use of beta-blockers or amiodarone in primary prevention seems to reduce its incidence significantly. There is growing evidence that POAF significantly increases the risk of stroke and global mortality in the long term; therefore, it should be managed in the same way as spontaneous atrial fibrillation. In this review, we will present the strongest evidence found so far and the most recent findings regarding the management of POAF, with a special focus on patients undergoing thoracic surgery for lung cancer.
Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System
Authors: Ahn Yujin , An Ju-Hyun , Yang Hae-Jun , Lee Dong Gil , Kim Jieun , Koh Hyebin , Park Young-Ho , Song Bong-Seok , Sim Bo-Woong , Lee Hong J. , Lee Jong-Hee , Kim Sun-Uk
Published: August 2021

Vascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured endothelial cells lack the capacity to interact with other somatic cell types, which is distinct from developing vascular cells in vivo. Recently, it was demonstrated that blood vessel organoids (BVOs) recreate the structure and functions of developing human blood vessels. However, the tissue-specific adaptability of BVOs had not been assessed in somatic tissues. Herein, we investigated whether BVOs infiltrate human cerebral organoids and form a blood–brain barrier. As a result, vascular cells arising from BVOs penetrated the cerebral organoids and developed a vessel-like architecture composed of CD31+ endothelial tubes coated with SMA+ or PDGFR+ mural cells. Molecular markers of the blood-brain barrier were detected in the vascularized cerebral organoids. We revealed that BVOs can form neural-specific blood-vessel networks that can be maintained for over 50 days.
Invadopodia, a Kingdom of Non-Receptor Tyrosine Kinases
Authors: Saha Trishna , Gil-Henn Hava
Published: August 2021

Non-receptor tyrosine kinases (NRTKs) are crucial mediators of intracellular signaling and control a wide variety of processes such as cell division, morphogenesis, and motility. Aberrant NRTK-mediated tyrosine phosphorylation has been linked to various human disorders and diseases, among them cancer metastasis, to which no treatment presently exists. Invasive cancer cells leaving the primary tumor use invadopodia, feet-like structures which facilitate extracellular matrix (ECM) degradation and intravasation, to escape the primary tumor and disseminate into distant tissues and organs during metastasis. A major challenge in metastasis research is to elucidate the molecular mechanisms and signaling pathways underlying invadopodia regulation, as the general belief is that targeting these structures can potentially lead to the eradication of cancer metastasis. Non-receptor tyrosine kinases (NRTKs) play a central role in regulating invadopodia formation and function, but how they coordinate the signaling leading to these processes was not clear until recently. Here, we describe the major NRTKs that rule invadopodia and how they work in concert while keeping an accurate hierarchy to control tumor cell invasiveness and dissemination.
Circulating myomiRs in Muscle Denervation: From Surgical to ALS Pathological Condition
Authors: Casola Irene , Scicchitano Bianca Maria , Lepore Elisa , Mandillo Silvia , Golini Elisabetta , Nicoletti Carmine , Barberi Laura , Dobrowolny Gabriella , Musarò Antonio
Published: August 2021

ALS is a fatal neurodegenerative disease that is associated with muscle atrophy, motoneuron degeneration and denervation. Different mechanisms have been proposed to explain the pathogenesis of the disease; in this context, microRNAs have been described as biomarkers and potential pathogenetic factors for ALS. MyomiRs are microRNAs produced by skeletal muscle, and they play an important role in tissue homeostasis; moreover, they can be released in blood circulation in pathological conditions, including ALS. However, the functional role of myomiRs in muscle denervation has not yet been fully clarified. In this study, we analyze the levels of two myomiRs, namely miR-206 and miR-133a, in skeletal muscle and blood samples of denervated mice, and we demonstrate that surgical denervation reduces the expression of both miR-206 and miR-133a, while miR-206 but not miR-133a is upregulated during the re-innervation process. Furthermore, we quantify the levels of miR-206 and miR-133a in serum samples of two ALS mouse models, characterized by different disease velocities, and we demonstrate a different modulation of circulating myomiRs during ALS disease, according to the velocity of disease progression. Moreover, taking into account surgical and pathological denervation, we describe a different response to increasing amounts of circulating miR-206, suggesting a hormetic effect of miR-206 in relation to changes in neuromuscular communication.
On “Decisions and Revisions Which a Minute Will Reverse”: Consciousness, The Unconscious and Mathematical Modeling of Thinking
Authors: Plotnitsky Arkady
Published: August 2021

This article considers a partly philosophical question: What are the ontological and epistemological reasons for using quantum-like models or theories (models and theories based on the mathematical formalism of quantum theory) vs. classical-like ones (based on the mathematics of classical physics), in considering human thinking and decision making? This question is only partly philosophical because it also concerns the scientific understanding of the phenomena considered by the theories that use mathematical models of either type, just as in physics itself, where this question also arises as a physical question. This is because this question is in effect: What are the physical reasons for using, even if not requiring, these types of theories in considering quantum phenomena, which these theories predict fully in accord with the experiment? This is clearly also a physical, rather than only philosophical, question and so is, accordingly, the question of whether one needs classical-like or quantum-like theories or both (just as in physics we use both classical and quantum theories) in considering human thinking in psychology and related fields, such as decision science. It comes as no surprise that many of these reasons are parallel to those that are responsible for the use of QM and QFT in the case of quantum phenomena. Still, the corresponding situations should be understood and justified in terms of the phenomena considered, phenomena defined by human thinking, because there are important differences between these phenomena and quantum phenomena, which this article aims to address. In order to do so, this article will first consider quantum phenomena and quantum theory, before turning to human thinking and decision making, in addressing which it will also discuss two recent quantum-like approaches to human thinking, that by M. G. D’Ariano and F. Faggin and that by A. Khrennikov. Both approaches are ontological in the sense of offering representations, different in character in each approach, of human thinking by the formalism of quantum theory. Whether such a representation, as opposed to only predicting the outcomes of relevant experiments, is possible either in quantum theory or in quantum-like theories of human thinking is one of the questions addressed in this article. The philosophical position adopted in it is that it may not be possible to make this assumption, which, however, is not the same as saying that it is impossible. I designate this view as the reality-without-realism, RWR, view and in considering strictly mental processes as the ideality-without-idealism, IWI, view, in the second case in part following, but also moving beyond, I. Kant’s philosophy.
A Comprehensive Review of EMI Filter Network Architectures: Synthesis, Optimization and Comparison
Authors: Dey Saikat , Mallik Ayan
Published: August 2021

This paper presents a volumetric comparison among three possible optimized three phase EMI filter structures, a three phase PFC converter used in cutting edge applications, such as avionics, space or shipboard power systems. The size minimization of each of the filter structures, described in the paper, was performed utilizing the volumetric optimization methodology proposed in the paper. This paper theoretically demonstrates the design steps for choosing the appropriate filter component values and number of filter stages to achieve the smallest volume of the DM filter stage for any given EMI filter structure. While the validation of the proposed design methodology was done through a MATLAB simulation, an experimental verification was also performed by designing and comparing the optimized EMI filter structures for a 2.3 kW proof-of-concept of a three-phase boost PFC converter for more electric aircraft (MEA) applications to comply with the stringent EMI requirements of the DO-160F standard.
Transient Voltage UWB Online Monitoring System for Insulation Failure Analysis and Fault Location of GIL
Authors: Zhang Ziwei , Ding Dengwei , He Liang , Liu Weidong , Bai Cuifen , Liu Junjun
Published: August 2021

Transient voltage generated in ultra-high voltage (UHV) transmission system has a severe impact on the insulation state of gas-insulated transmission lines (GIL). In order to monitor the transient voltage process occurring in UHV GIL during operation, this paper constructs a transient voltage ultra-wideband (UWB) online monitoring system based on capacitive voltage division. This system has been applied in an 1100 kilovolt (kV) GIL utility tunnel project. It can be used to analyze the characteristics of the recorded transient voltage waveforms for distinguishing different types of insulation failure. In this paper, through the case studies in time domain and time–frequency domain, the case of SF6 gap breakdown and that of post insulator flashover have differentiated characteristics in instantaneous frequency. Additionally, a case of secondary discharge is successfully estimated through the time–frequency distribution of the transient voltage. In order to find the malfunctioning chamber of GIL rapidly, a two-terminal TW-based fault location method based on this monitoring system is developed in this paper. The case study validates the locating accuracy which directly support for shortening the maintenance time of GIL.
A Novel Island Detection Threshold Setting Using Phasor Measurement Unit Voltage Angle in a Distribution Network
Authors: Arefin Ahmed Amirul , Hasan Khairul Nisak Binti Md. , Othman Mohammad Lutfi , Romlie Mohd Fakhizan , Saad Nordin , Nor Nursyarizal Bin Mohd , Abdullah Mohd Faris
Published: August 2021

Islanding detection needs are becoming a pivotal constituent of the power system, since the penetration of distributed generators in the utility power system is continually increasing. Accurate threshold setting is an integral part of the island detection scheme since an inappropriate threshold might cause a hazardous situation. This study looked at the islanding conditions as well as two transient faults, such as a single line to ground fault and a three-phase balance fault, to assess the event distinguishing ability of the proposed method. Therefore, the goal of this research was to determine the threshold of the island if the distributed generator (DG) capacity is greater than the connected feeder load, which is the over-frequency island condition, and if the DG capacity is less than the connected feeder load, which is the under-frequency island condition. The significance of this research work is to propose a new island detection threshold setting method using the slip angle and acceleration angle that comes from phasor measurement unit (PMU) voltage angle data. The proposed threshold setting method was simulated in the PowerWorld simulator on a modified IEEE 30 bus system equipped with DG. There are three different interconnection scenarios in the test system and the performance of the proposed method shows that getting the island threshold for all the scenarios requires a single time step or 20 mile seconds after incepting an island into the network. In addition, it can distinguish between the real islanding threshold and the transient faults threshold.
Machine Learning-Based Fault Detection and Diagnosis of Faulty Power Connections of Induction Machines
Authors: Gonzalez-Jimenez David , del-Olmo Jon , Poza Javier , Garramiola Fernando , Sarasola Izaskun
Published: August 2021

Induction machines have been key components in the industrial sector for decades, owing to different characteristics such as their simplicity, robustness, high energy efficiency and reliability. However, due to the stress and harsh working conditions they are subjected to in many applications, they are prone to suffering different breakdowns. Among the most common failure modes, bearing failures and stator winding failures can be found. To a lesser extent, High Resistance Connections (HRC) have also been investigated. Motor power connection failure mechanisms may be due to human errors while assembling the different parts of the system. Moreover, they are not only limited to HRC, there may also be cases of opposite wiring connections or open-phase faults in motor power terminals. Because of that, companies in industry are interested in diagnosing these failure modes in order to overcome human errors. This article presents a machine learning (ML) based fault diagnosis strategy to help maintenance assistants on identifying faults in the power connections of induction machines. Specifically, a strategy for failure modes such as high resistance connections, single phasing faults and opposite wiring connections has been designed. In this case, as field data under the aforementioned faulty events are scarce in industry, a simulation-driven ML-based fault diagnosis strategy has been implemented. Hence, training data for the ML algorithm has been generated via Software-in-the-Loop simulations, to train the machine learning models.