bluuebunny/crossref_metadata_2025 huggingface dataset:
Southeast Asia’s environmental challenges: emergence of new contaminants and advancements in testing methods
Authors: Bhagat Jacky , Singh Nisha , Shimada Yasuhito
Published: February 2024

Emerging contaminants, including pharmaceuticals, personal care products, microplastics, and per- and poly-fluoroalkyl substances, pose a major threat to both ecosystems and human health in Southeast Asia. As this region undergoes rapid industrialization and urbanization, the increasing presence of unconventional pollutants in water bodies, soil, and various organisms has become an alarming concern. This review comprehensively examines the environmental challenges posed by emerging contaminants in Southeast Asia and recent progress in toxicity testing methods. We discuss the diverse range of emerging contaminants found in Southeast Asia, shedding light on their causes and effects on ecosystems, and emphasize the need for robust toxicological testing methods. This review is a valuable resource for researchers, policymakers, and environmental practitioners working to mitigate the impacts of emerging contaminants and secure a sustainable future for Southeast Asia.
Discrete Element Method–Multibody Dynamics Coupling Simulation and Experiment of Rotary Tillage and Ridging Process for Chili Pepper Cultivation
Authors: Yang Zhikai , Zhang Keping , Zhang Yang , An Jing
Published: February 2024

Rotary tillage, ridging, and mulching are commonly used cultivation methods for crops such as chili peppers, tomatoes, and strawberries in the arid regions of Northwest China. An integrated machine for rotary tillage, ridging, and mulching was designed by considering the growth characteristics of pepper root systems and the agronomic requirements of ridge beds. The structural parameters and their value ranges for key components such as the rotary tillage device and the ridging device were determined. By introducing the Bonding contact parameter, the soil cohesion between soil particles during the process of rotary tillage and ridging can be simulated. A coupled simulation model using the Discrete Element Method (DEM) and Multibody Dynamics (MBD) is established. The experimental factors selected were rotary tillage depth, ridging roller speed, and machine forward speed. The evaluation indexes were the traction resistance of the stemming roller and the soil compactness of the ridges. A response surface Box–Behnken Design test was carried out to obtain the best working parameters of the rotary tillage and ridging process for chili pepper cultivation as follows: the rotary tillage depth was 176 mm, the ridging roller speed was 283.71 r/min, and the machine forward speed was 0.55 m/s. Field experiments with optimal parameters showed that the ridge top width was 549.2 mm, the ridge bottom width was 750.5 mm, the ridge height was 222.9 mm, the ridge spacing was 1173.1 mm, the surface smoothness of the ridge was 12.3 mm, the width of soil covering the film edge was 76.3 mm, the stability coefficients of the ridge size parameters were all above 91.73%, and the soil compactness after operation was 60.82 KPa. All indicators meet the requirements for the rotary tillage and ridging cultivation of chili peppers in arid regions, providing reference for the design of rotary tillage and ridging mulching implements and the development of sustainable agriculture.
Susceptibility of Fall Armyworm Field Populations to Vip3Aa/Cry Bt Maize in a Tropical Agricultural Region
Authors: Silva Alisson Franco T. , Silva Luciana B. , Malaquias José B. , Salustino Angélica S. , Correia Neto Domingos Francisco , Pacheco Daniel M. , Fragoso Daniel B. , Pereira Eliseu J. G.
Published: February 2024

Fall armyworm (FAW, Spodoptera frugiperda) is a polyphagous and migratory lepidopteran pest insect in field crops and is notoriously invasive worldwide. In large portions of the Americas, its populations are managed using transgenic maize or cotton varieties producing insecticidal proteins from Bacillus thuringiensis (Bt), primarily Vip3Aa pyramided with Cry Bt proteins. We determined the susceptibility of FAW field populations from locations pressured with such maize hybrids for over five years. We used time–mortality bioassays with F1 third-instar larvae of six geographically distinct populations collected in maize fields of a tropical agricultural region encompassing four Brazilian states. We maintained the neonate progeny from the field populations on an artificial diet until the third instar, and then determined their survival curves on the foliage of three Vip3Aa/Cry-producing Bt maize hybrids. Death of the mid-size, third-instar FAWs occurred relatively rapidly, with larval mortality rates reaching 98–100% in less than five days regardless of Bt maize hybrid. However, median survival time (ST50) for the larvae differed among the populations, with the lowest and highest ST50 values occurring for PI-Cr (42 h, 1.75 d) and PI-Ur populations (66–90 h, 2.75–3.75 h), respectively. Therefore, the F1 third-instar larvae of FAW populations were largely susceptible to Vip3Aa/Cry-producing maize foliage, and the most contrasting susceptibility occurred in the insects from Piauí state, Brazil. These results indicate that progeny of FAWs from areas highly pressured with Vip3Aa/Cry Bt maize hybrids are killed on maize foliage producing Vip3Aa and Cry Bt proteins despite field reports of increased leaf damage by the larvae in some locations. This research informs decision making for Bt-crop resistance management by producers, technicians, and researchers in local, regional, and world agriculture.
Histomorphology of Chorionic Villi of Term Placentae of Mothers Exposed to Retroviral and Hepatitis B Viruses
Authors: Ahenkorah John , Opoku-Nyarko Stephen , Adutwum-Ofosu Kevin Kofi , Hottor Bismarck , Twasam Joana , Afutu Emmanuel , Nyadroh Clement , Kotey Fleischer C. N. , Donkor Eric S. , Dayie Nicholas T. K. D. , Tette Edem M. A. , Tetteh-Quarcoo Patience B.
Published: February 2024

Retroviral and hepatitis B infections can be potential threats to foetomaternal health through inducing distortions of the architecture and structure of the placenta. Improved insights into the effects of these infections on placental morphology would be integral to our understanding of maternal and neonatal health. Aim: To histomorphologically and stereologically investigate selected placental structures in virus-infected (HIV [human immunodeficiency virus] and hepatitis B virus [HBV]) and uninfected women at term. Method: This cross-sectional study involved the screening of 237 placentae collected at term (38 ± 2 weeks) from the maternity delivery units and surgical theatres of the LEKMA and Weija/Gbawe Municipal Hospitals in Accra. Venous blood samples from the umbilical vein and placenta basal plate blood were screened for HIV, HBV, and hepatitis C virus (HCV) using serological test kits (RDT). A total of 34 placentae were selected, comprising 20 cases and 14 controls that were gestational age-matched. Using stereology and a systematic random sampling technique with test point and intersection counting of photomicrographs, the mean volume densities of syncytial knots, syncytial denudations, foetal capillaries, and intervillous spaces of the placentae were estimated on a total of 2720 photomicrographs. Results: On stereological assessment, there was a statistically significant difference in the mean volume densities of syncytial knots (HIV-infected = 0.562 ± 0.115, HBV-infected = 0.516 ± 0.090, control group = 0.171 ± 0.018, p = 0.001), syncytial denudations (HIV-infected = 0.121 ± 0.022, HBV-infected = 0.111 ± 0.016, control group = 0.051 ± 0.00, p = 0.004), and foetal capillaries (HIV-infected = 0.725 ± 0.152, HBV-infected = 0.902 ± 0.078, control group = 0.451 ± 0.064, p = 0.006) among the different groups of placentae (control) at term. A statistically significant decrease in intervillous space (p = 0.022) was recorded in HBV-infected placentae compared to the control (from 15.450 ± 1.075 to 11.32 ± 0.952). Conclusion: Placental viral infections might lead to significant increases in syncytial knots, foetal capillaries, and syncytial denuded areas of the chorionic villi and a significant decrease in intervillous spaces. This finding could signify evidence of advanced gestation, placental malperfusion, hypermaturity of the placenta, and a possible vertical transmission of the viral antigen to the foetus, which may be crucial in understanding perinatal outcomes.
Immunophysiological State of Dogs According to the Immunoregulatory Index of Their Blood and Spleens
Authors: Dunaievska Oksana , Sokulskyi Ihor , Radzykhovskii Mykola , Gutyj Bogdan , Dyshkant Olga , Khomenko Zoriana , Brygadyrenko Viktor
Published: February 2024

In this study, the immunological characteristics of a dog’s body were established, allowing for a quick reaction to any changes in the immune status and the development of an immunodeficiency state. The immunoregulatory blood index was determined to indicate the ratio of T-helpers and T-suppressors. The immunoregulatory index of the spleen was determined as the ratio of CD4+ cells to CD8+ cells in the field of view of a microscope (eyepiece 10, objective 40) after obtaining histological preparations according to generally accepted methods. It was found that the number of T-helpers decreased by 0.13 × 1012/L, while the number of T-suppressors increased non-significantly by 0.01 × 1012/L after intensive exercise during tasks. The immunoregulatory blood index of dogs was 2.1 ± 0.1 and 1.7 ± 0.13 before and after intensive exercise, respectively. Lymphocytes with markers CD4+ and CD8+ were located almost all in the white pulp; in the red pulp, they were found alone, and their share was 3.4% and 1.9%, respectively. Lymphocytes with CD4+ markers in the spleen’s white pulp were mainly concentrated in lymphoid nodules (60.7%), of which 20.1% were focused on the marginal zone, and slightly less in the light center (19.4%) and the periarterial zone (18.1%). Lymphocytes with CD8+ markers in the spleen’s white pulp were also mainly concentrated in lymphoid nodules, but their number was 8.1% higher (68.8%). The immunoregulatory index of the spleen is 1.9. These findings emphasize the need for the assessment of the immunoregulatory index in service dogs to prevent the development of secondary immunodeficiency and allow them to properly perform their official duties.
Spatiotemporal Patterns of Reptile and Amphibian Road Fatalities in a Natura 2000 Area: A 12-Year Monitoring of the Lake Karla Mediterranean Wetland
Authors: Kouris Alexandros D. , Christopoulos Apostolos , Vlachopoulos Konstantinos , Christopoulou Aikaterini , Dimitrakopoulos Panayiotis G. , Zevgolis Yiannis G.
Published: February 2024

The pervasive expansion of human-engineered infrastructure, particularly roads, has fundamentally reshaped landscapes, profoundly affecting wildlife interactions. Wildlife-vehicle collisions, a common consequence of this intricate interplay, frequently result in fatalities, extending their detrimental impact within Protected Areas (PAs). Among the faunal groups most susceptible to road mortality, reptiles and amphibians stand at the forefront, highlighting the urgent need for global comprehensive mitigation strategies. In Greece, where road infrastructure expansion has encroached upon a significant portion of the nation’s PAs, the plight of these road-vulnerable species demands immediate attention. To address this critical issue, we present a multifaceted and holistic approach to investigating and assessing the complex phenomenon of herpetofauna road mortality within the unique ecological context of the Lake Karla plain, a rehabilitated wetland complex within a PA. To unravel the intricacies of herpetofauna road mortality in the Lake Karla plain, we conducted a comprehensive 12-year investigation from 2008 to 2019. Employing a combination of statistical modeling and spatial analysis techniques, we aimed to identify the species most susceptible to these encounters, their temporal and seasonal variations, and the ecological determinants of their roadkill patterns. We documented a total of 340 roadkill incidents involving 14 herpetofauna species in the Lake Karla’s plain, with reptiles, particularly snakes, being more susceptible, accounting for over 60% of roadkill occurrences. Moreover, we found that environmental and road-related factors play a crucial role in influencing roadkill incidents, while spatial analysis techniques, including Kernel Density Estimation, the Getis-Ord Gi*, and the Kernel Density Estimation plus methods revealed critical areas, particularly in the south-eastern region of Lake Karla’s plain, offering guidance for targeted interventions to address both individual and collective risks associated with roadkill incidents.
Long-Term Effects of Pre-Weaning Individual or Pair Housing of Dairy Heifer Calves on Subsequent Growth and Feed Efficiency
Authors: Riesgraf Kaylee A. , Weigel Kent A. , Akins Matthew S. , Van Os Jennifer M. C.
Published: February 2024

Our objective in this exploratory study was to evaluate the long-term impacts of pre-weaning social isolation vs. contact on subsequent growth and feed efficiency of Holstein heifers. As pre-weaned calves, 41 heifers were housed individually (n = 15 heifers) or in pairs (n = 13 pairs; 26 heifers). At 18 months of age, heifers were blocked by body weight and randomly assigned to one of three pens within a block (six to eight heifers per pen; six pens total), with original pairs maintained. Body weight (BW), hip height and width, and chest girth were measured at the start and end of the study. Each pen was given 3 days of access to a GreenFeed greenhouse gas emissions monitor to assess potential physiological differences between treatments in enteric methane emissions or behavioral differences in propensity to approach a novel object. During the 9-week study, heifers were fed a common diet containing 62.3% male-sterile corn silage, 36.0% haylage, 0.7% urea, and 1.0% mineral (DM basis). To calculate daily feed intake, as-fed weights and refusals were recorded for individual heifers using Calan gates. Feed samples were collected daily, composited by week, and dried to calculate dry matter intake (DMI). Feed refusal and fecal samples were collected on 3 consecutive days at 3 timepoints, composited by heifer, dried, and analyzed to calculate neutral detergent fiber (NDF), organic matter (OM), and DM digestibility. Feed efficiency was calculated as feed conversion efficiency (FCE; DMI/average daily gain [ADG]) and residual feed intake (RFI; observed DMI-predicted DMI). Paired and individually housed heifers did not differ in DMI, ADG, FCE, or RFI. Although no differences were found in initial or final hip height, hip width, or chest girth, heifers which had been pair-housed maintained a greater BW than individually housed heifers during the trial. Methane production, intensity, and yield were similar between treatments. Pre-weaning paired or individual housing did not impact the number of visits or latency to approach the GreenFeed; approximately 50% of heifers in each treatment visited the GreenFeed within 8 h of exposure. Digestibility of OM, DM, and NDF were also similar between housing treatments. In conclusion, pre-weaning pair housing had no adverse effects on growth, feed efficiency, or methane emissions at 18 to 20 months of age.
Analysis of Genetic Diversity and Structure of Eight Populations of Nerita yoldii along the Coast of China Based on Mitochondrial COI Gene
Authors: Jiang Senping , Li Zhenhua , Li Jiji , Xu Kaida , Ye Yingying
Published: February 2024

Nerita yoldii is a euryhaline species commonly found in the intertidal zone. To investigate the genetic diversity of 233 N. yoldii individuals from eight locations along the coast of China, we utilized the mitochondrial COI gene as a molecular marker. A total of 34 haplotypes were detected, exhibiting a mean haplotype diversity (Hd) of 0.5915 and a mean nucleotide diversity (Pi) of 0.0025, indicating high levels of genetic diversity among all populations. An analysis of molecular variance (AMOVA) indicated that the primary source of genetic variation occurs within populations. In addition, neutral tests and mismatch analyses suggested that N. yoldii populations may have experienced bottleneck events. Moderate genetic differentiation was observed between Xiapu and other populations, excluding the Taizhou population, and may be attributed to the ocean currents. Intensively studying the genetic variation and population structure of N. yoldii populations contributes to understanding the current population genetics of N. yoldii in the coastal regions of China. This not only provides a reference for the study of other organisms in the same region but also lays the foundation for the systematic evolution of the Neritidae family.
Effect of Exercise Conditioning on Countering the Effects of Obesity and Insulin Resistance in Horses—A Review
Authors: Pratt-Phillips Shannon
Published: February 2024

Obesity is an important health concern in horses, along with humans and companion animals. Adipose tissue is an inflammatory organ that alters the insulin-signaling cascade, ultimately causing insulin dysregulation and impaired glucose metabolism. These disruptions can increase the risk of metabolic disease and laminitis in horses and may also impact energy metabolism during exercise. A single bout of exercise, along with chronic exercise conditioning, increases insulin sensitivity and glucose disposal via both contraction- and insulin-mediated glucose uptake pathways. Regular exercise also increases calorie expenditure, which can facilitate weight (as body fat) loss. This paper explores the metabolic pathways affected by adiposity, as well as discusses the impact of exercise on insulin metabolism in horses.
Potential Antifungal Effect of Copper Oxide Nanoparticles Combined with Fungicides against Botrytis cinerea and Fusarium oxysporum
Authors: Parada Javiera , Tortella Gonzalo , Seabra Amedea B. , Fincheira Paola , Rubilar Olga
Published: February 2024

Copper oxide nanoparticles (NCuO) have emerged as an alternative to pesticides due to their antifungal effect against various phytopathogens. Combining them with fungicides represents an advantageous strategy for reducing the necessary amount of both agents to inhibit fungal growth, simultaneously reducing their environmental release. This study aimed to evaluate the antifungal activity of NCuO combined with three fungicide models separately: Iprodione (IPR), Tebuconazole (TEB), and Pyrimethanil (PYR) against two phytopathogenic fungi: Botrytis cinerea and Fusarium oxysporum. The fractional inhibitory concentration (FIC) was calculated as a synergism indicator (FIC ≤ 0.5). The NCuO interacted synergistically with TEB against both fungi and with IPR only against B. cinerea. The interaction with PYR was additive against both fungi (FIC > 0.5). The B. cinerea biomass was inhibited by 80.9% and 93% using 20 mg L−1 NCuO + 1.56 mg L−1 TEB, and 40 mg L−1 NCuO + 12 µg L−1 IPR, respectively, without significant differences compared to the inhibition provoked by 160 mg L−1 NCuO. Additionally, the protein leakage and nucleic acid release were also evaluated as mechanisms associated with the synergistic effect. The results obtained in this study revealed that combining nanoparticles with fungicides can be an adequate strategy to significantly reduce the release of metals and agrochemicals into the environment after being used as antifungals.
Research on the Terrain Characteristics of Changbai Mountain and Their Impact on Precipitation and Wind Distribution
Authors: Liang Li , Ai Wanxiu , Yang Xiaodan , Zhao Luqiang
Published: February 2024

The terrain of Changbai Mountain has great influence on the distribution of atmospheric flows and the occurrence and development of precipitation. However, quantitative studies on the real terrain characteristics and the terrain effect on precipitation distribution in this region are scant at present. This study quantitatively analyzes the regional characteristic of topographic perturbations and the relationship between terrain, wind, and precipitation in Changbai Mountain region by using a spectral analysis of the two-dimensional discrete cosine transform. Three domains with relatively heavy summer precipitation are selected as the study region. The results indicate that the overall terrain of the Changbai Mountain region exhibits anisotropic characteristics. The terrain spectra of domain B are less than those of domains A and C across the whole wavelength (λ) bands, indicating that the large-scale topographic perturbations of domain B are relatively weak. The largest topographic spectral peak of domain C shows the most pronounced undulation of terrain among the three domains. The dominant wavelengths of terrain height variance for domains A and C, both close to the respective maximum wavelengths, indicate more prominent large-scale topographic perturbations. For domain A, the variation of the precipitation spectra is consistent with that of the wind spectra at the wavelength bands of λ < 390 km, showing a high correlation between wind field and the occurrence of rainfall. The inverse relationship at larger wavelengths indicates that multiple factors contribute to the occurrence of rainfall. For domain B, there is consistency in the fluctuations of terrain spectra, precipitation spectra, and wind spectra at the wavelength bands of λ < 278.3 km, implying that the smaller-scale terrain has an important effect on the occurrence of summer precipitation. For domain C, the variations of terrain spectra, precipitation spectra, and wind spectra are almost consistent across the whole wavelength bands, indicating that the large-scale terrain and minor terrain both play a crucial role in atmospheric uplift and the occurrence and development of summer rainfall.
Seasonal Features of the Ionospheric Total Electron Content Response at Low Latitudes during Three Selected Geomagnetic Storms
Authors: Bojilova Rumiana , Mukhtarov Plamen
Published: February 2024

In the present paper, the response of the ionospheric Total Electron Content (TEC) at low latitudes during several geomagnetic storms occurring in different seasons of the year is investigated. In the analysis of the ionospheric response, the following three geomagnetic events were selected: (i) 23–24 April 2023; (ii) 22–24 June 2015 and (iii) 16 December 2006. Global TEC data were used, with geographic coordinates recalculated with Rawer’s modified dip (modip) latitude, which improved the accuracy of the representation of the ionospheric response at low and mid-latitudes. By decomposition of the zonal distribution of the relative deviation of the TEC values from the hourly medians, the spatial distribution of the anomalies, the dependence of the response on the local time and their evolution during the selected events were analyzed. As a result of the study, it was found that the positive response (i.e., an increase in electron density relative to quiet conditions) in low latitudes occurs at the modip latitudes 30° N and 30° S. An innovative result related to the observed responses during the considered events is that they turn out to be relatively stationary. The longitude variation in the observed maxima changes insignificantly during the storms. Depending on the season, there is an asymmetry between the two hemispheres, which can be explained by the differences in the meridional neutral circulation in different seasons.
Ocean Temperatures Do Not Account for a Record-Setting Winter in the U.S. West
Authors: LaPlante Matthew , Deng Liping , Dalanhese Luthiene , Wang Shih-Yu
Published: February 2024

The record-setting winter of 2022–2023 came as an answer to both figurative and literal prayers for political leaders, policy makers, and water managers reliant on snowpacks in the Upper Colorado River Basin, a vital source of water for tens of millions of people across the Western United States. But this “drought-busting” winter was not well-predicted, in part because while interannual patterns of tropical ocean temperatures have a well-known relationship to precipitation patterns across much of the American West, the Upper Colorado is part of a liminal region where these connections tend to be comparatively weak. Using historical sea surface temperature and snowpack records, and leveraging a long-term cross-basin relationship to extend the timeline for evaluation, this analysis demonstrates that the 2022–2023 winter did not present in accordance with other high-snowpack winters in this region, and that the associative pattern of surface temperatures in the tropical Pacific, and snow water equivalent in the regions that stored and supplied most of the water to the Colorado River during the 2022–2023 winter, was not substantially different from a historically incoherent arrangement of long-term correlation. These findings suggest that stochastic variability plays an outsized role in influencing water availability in this region, even in extreme years, reinforcing the importance of other trends to inform water policy and management.
The Relationship between the Typhoons Affecting South China and the Pacific Decadal Oscillation
Authors: Qin Weijian , Cai Yuexing , He Liyang
Published: February 2024

Using typhoon data from the Shanghai Typhoon Institute of the China Meteorological Administration, the Japan Meteorological Agency’s annual Pacific decadal oscillation (PDO) index, and NCEP/NCAR reanalysis data from 1951 to 2021, correlation and composite analyses were carried out to study the relationship between the variability among tropical cyclones of different magnitudes affecting South China and the PDO. The results show that there is an obvious out-of-phase relationship between the proportion of tropical cyclones reaching a typhoon-level intensity or above in South China and the PDO index. When the PDO is in a cold (warm) phase, the sea surface temperature in the eastern and central equatorial Pacific is cold (warm), similar to the eastern Pacific La Niña (El Niño) phenomenon, and the SST in the eastern and western tropical Pacific Ocean shows a negative (positive) gradient; the subtropical high in the western Pacific Ocean is weaker (stronger) than normal, with the western ridge point to the east (west), and the 500 hPa geopotential height in the South China Sea and the area east of the Philippines is weaker (stronger), which is conducive to (unfavorable to) the formation of a monsoon trough; and the westerly (easterly) winds at high altitudes and the southwesterly (northeasterly) winds at low altitudes from the South China Sea to the Philippines are abnormally strong, and a positive (negative) vorticity at low altitudes, a low (high) sea level pressure, and strong (weak) convection are shown. These conditions are favorable (unfavorable) for the intensification of typhoons affecting South China, and as a result, the number of tropical cyclones reaching the level of typhoons or above account for a greater (smaller) proportion of those affecting South China.
The Constrained Disorder Principle May Account for Consciousness
Authors: Sigawi Tal , Hamtzany Omer , Shakargy Josef Daniel , Ilan Yaron
Published: February 2024

There is still controversy surrounding the definition and mechanisms of consciousness. The constrained disorder principle (CDP) defines complex systems by their dynamic borders, limiting their inherent disorder. In line with the CDP, the brain exhibits a disorder bounded by dynamic borders essential for proper function, efficient energy use, and life support under continuous perturbations. The brain’s inherent variability contributes to its adaptability and flexibility. Neuronal signal variability challenges the association of brain structures with consciousness and methods for assessing consciousness. The present paper discusses some theories about consciousness, emphasizing their failure to explain the brain’s variability. This paper describes how the CDP accounts for consciousness’s variability, complexity, entropy, and uncertainty. Using newly developed second-generation artificial intelligence systems, we describe how CDP-based platforms may improve disorders of consciousness (DoC) by accounting for consciousness variability, complexity, entropy, and uncertainty. This platform could be used to improve response to current interventions and develop new therapeutic regimens for patients with DoC in future studies.
Exploring Brain Dynamics via EEG and Steady-State Activation Map Networks in Music Composition
Authors: Gu Xiaohu , Jiang Leqi , Chen Hao , Li Ming , Liu Chang
Published: February 2024

In recent years, the integration of brain–computer interface technology and neural networks in the field of music generation has garnered widespread attention. These studies aimed to extract individual-specific emotional and state information from electroencephalogram (EEG) signals to generate unique musical compositions. While existing research has focused primarily on brain regions associated with emotions, this study extends this research to brain regions related to musical composition. To this end, a novel neural network model incorporating attention mechanisms and steady-state activation mapping (SSAM) was proposed. In this model, the self-attention module enhances task-related information in the current state matrix, while the extended attention module captures the importance of state matrices over different time frames. Additionally, a convolutional neural network layer is used to capture spatial information. Finally, the ECA module integrates the frequency information learned by the model in each of the four frequency bands, mapping these by learning their complementary frequency information into the final attention representation. Evaluations conducted on a dataset specifically constructed for this study revealed that the model surpassed representative models in the emotion recognition field, with recognition rate improvements of 1.47% and 3.83% for two different music states. Analysis of the attention matrix indicates that the left frontal lobe and occipital lobe are the most critical brain regions in distinguishing between ‘recall and creation’ states, while FP1, FPZ, O1, OZ, and O2 are the electrodes most related to this state. In our study of the correlations and significances between these areas and other electrodes, we found that individuals with musical training exhibit more extensive functional connectivity across multiple brain regions. This discovery not only deepens our understanding of how musical training can enhance the brain’s ability to work in coordination but also provides crucial guidance for the advancement of brain–computer music generation technologies, particularly in the selection of key brain areas and electrode configurations. We hope our research can guide the work of EEG-based music generation to create better and more personalized music.
Dating Violence and Emotional Dependence in University Students
Authors: Castillo-Gonzáles Mayra , Mendo-Lázaro Santiago , León-del-Barco Benito , Terán-Andrade Emilio , López-Ramos Víctor-María
Published: February 2024

The aim of this study was to analyse the relationship between dating violence and emotional dependence in young university students in Ecuador by identifying differences based on sex. Using purposive non-probabilistic sampling, 3203 students were selected, of which 35.7% were men and 64.3% were women aged 16 to 48 (M = 21.50; SD = 2.82). Two psychological scales were applied: Questionnaire on Violence in Couples (CUVINO) and Questionnaire on Emotional Dependence (EDQ). According to the results obtained, based on sex, it was found that dating violence is a severe problem that both men and women experience. With regard to emotional dependence, women scored higher than men. In addition, emotional dependence was linked to dating violence. The implications of the results obtained for further research and for prevention and intervention programmes are presented, and the strengths and limitations of this study are detailed.
Precision Medicine for Nasopharyngeal Cancer—A Review of Current Prognostic Strategies
Authors: Suryani Luvita , Lee Hazel P. Y. , Teo Wei Keat , Chin Zhi Kang , Loh Kwok Seng , Tay Joshua K.
Published: February 2024

Nasopharyngeal carcinoma (NPC) is an Epstein–Barr virus (EBV) driven malignancy arising from the nasopharyngeal epithelium. Current treatment strategies depend on the clinical stage of the disease, including the extent of the primary tumour, the extent of nodal disease, and the presence of distant metastasis. With the close association of EBV infection with NPC development, EBV biomarkers have shown promise in predicting treatment outcomes. Among the omic technologies, RNA and miRNA signatures have been widely studied, showing promising results in the research setting to predict treatment response. The transformation of radiology images into measurable features has facilitated the use of radiomics to generate predictive models for better prognostication and treatment selection. Nonetheless, much of this work remains in the research realm, and challenges remain in clinical implementation.
Liver Transplantation for Hepatocellular Carcinoma beyond the Milan Criteria: A Specific Role for Living Donor Liver Transplantation after Neoadjuvant Therapy
Authors: Rohland Oliver , Freye Lea , Schwenk Laura , Ali-Deeb Aladdin , Ardelt Michael , Bauschke Astrid , Settmacher Utz , Rauchfuß Falk , Dondorf Felix
Published: February 2024

Purpose: This study was designed to elucidate the various new classifications and the use of LDLT and bridging therapy for HCC in this context beyond the Milan criteria (MC). Methods: The clinical data of patients with HCC outside the MC who underwent LT at Jena University between January 2007 and August 2023 were retrospectively analysed. Eligible patients were classified according to various classification systems. Clinicopathological features, overall and disease-free survival rates were compared between LT and LDLT within the context of bridging therapy. The Results: Among the 245 patients analysed, 120 patients did not meet the MC, and 125 patients met the MC. Moreover, there were comparable overall survival rates between patients outside the MC for LT versus LDLT (OS 44.3 months vs. 28.3 months; 5-year survival, 56.4% vs. 40%; p = 0.84). G3 tumour differentiation, the presence of angioinvasion and lack of bridging were statistically significant risk factors for tumour recurrence according to univariate and multivariate analyses (HR 6.34; p = 0.0002; HR 8.21; p < 0.0001; HR 7.50; p = 0.0001). Bridging therapy before transplantation provided a significant survival advantage regardless of the transplant procedure (OS: p = 0.008; DFS: p < 0.001). Conclusions: Patients with HCC outside the MC who underwent LT or LDLT had worse outcomes compared to those of patients who met the MC but still had a survival advantage compared to patients without transplantation. Nevertheless, such patients remain disadvantaged on the waiting list, which is why LDLT represents a safe alternative to LT and should be considered in bridged HCC patients because of differences in tumour differentiation, size and tumour marker dynamics.
Incidence and Mortality of Uveal Melanoma in Hungary: A Nationwide Study
Authors: Tóth Gábor , Muzsik Béla , Szajkó Attila , Kerber Pál , Dinya Elek , Csákány Béla , Nagy Zoltán Zsolt , Németh János
Published: February 2024

Uveal melanoma (UM) is the most common primary malignant ocular tumour in adults, although its epidemiology in Central and Eastern Europe is unclear. This study aimed to analyse the incidence and all-cause mortality of UM in Hungary. This nationwide, retrospective, longitudinal study used data from the National Health Insurance Fund and included patients aged ≥18 years who were newly diagnosed with UM (ICD-10 C69.3 or C69.4) between 1 January 2012 and 31 December 2021. Age-standardised incidence and all-cause mortality rates were calculated using European Standard Population data from 2013. We identified 88 and 70 new patients with UM in 2012 and 2021, respectively, showing an almost stable trend. Age-standardised incidence rates varied between 6.40 and 10.96/1,000,000 person-years (PYs) during the analysed period. The highest age-standardised incidence was detected among men (13.38/1,000,000 PYs) in 2015. All-cause mortality decreased from 4.72/1,000,000 PYs to 0.79/1,000,000 PYs between 2012 and 2021. In conclusion, the UM incidence rate in Hungary is comparable to European incidence rates. The incidence did not markedly change, whereas all-cause mortality decreased during the study period, but this decline could not be attributed to improved treatment modalities for primary tumours and metastatic UM.
Long-Term Outcomes of Ablative Carbon-Ion Radiotherapy for Central Non-Small Cell Lung Cancer: A Single-Center, Retrospective Study
Authors: Aoki Shuri , Ishikawa Hitoshi , Nakajima Mio , Yamamoto Naoyoshi , Mori Shinichiro , Wakatsuki Masaru , Okonogi Noriyuki , Murata Kazutoshi , Tada Yuji , Mizobuchi Teruaki , Yoshino Ichiro , Yamada Shigeru
Published: February 2024

The aim of this study is to assess the efficacy and safety of ablative carbon ion radiotherapy (CIRT) for early stage central non-small cell lung cancer (NSCLC). We retrospectively reviewed 30 patients who had received CIRT at 68.4 Gy in 12 fractions for central NSCLC in 2006–2019. The median age was 75 years, and the median Karnofsky Performance Scale score was 90%. All patients had concomitant chronic obstructive pulmonary disease, and 20 patients (67%) were considered inoperable. In DVH analysis, the median lung V5 and V20 were 15.5% and 10.4%, and the median Dmax, D0.5cc, D2cc of proximal bronchial tree was 65.6 Gy, 52.8 Gy, and 10.0 Gy, respectively. At a median follow-up of 43 months, the 3-year overall survival, disease-specific survival, and local control rates were 72.4, 75.8, and 88.7%, respectively. Two patients experienced grade 3 pneumonitis, but no grade ≥3 adverse events involving the mediastinal organs occurred. Ablative CIRT is feasible and effective for central NSCLC and could be considered as a treatment option, especially for patients who are intolerant of other curative treatments.
The Future of Targeted Therapy for Leiomyosarcoma
Authors: Denu Ryan A. , Dann Amanda M. , Keung Emily Z. , Nakazawa Michael S. , Nassif Haddad Elise F.
Published: February 2024

Leiomyosarcoma (LMS) is an aggressive subtype of soft tissue sarcoma that arises from smooth muscle cells, most commonly in the uterus and retroperitoneum. LMS is a heterogeneous disease with diverse clinical and molecular characteristics that have yet to be fully understood. Molecular profiling has uncovered possible targets amenable to treatment, though this has yet to translate into approved targeted therapies in LMS. This review will explore historic and recent findings from molecular profiling, highlight promising avenues of current investigation, and suggest possible future strategies to move toward the goal of molecularly matched treatment of LMS. We focus on targeting the DNA damage response, the macrophage-rich micro-environment, the PI3K/mTOR pathway, epigenetic regulators, and telomere biology.
A Comparison of 7 Tesla MR Spectroscopic Imaging and 3 Tesla MR Fingerprinting for Tumor Localization in Glioma Patients
Authors: Lazen Philipp , Lima Cardoso Pedro , Sharma Sukrit , Cadrien Cornelius , Roetzer-Pejrimovsky Thomas , Furtner Julia , Strasser Bernhard , Hingerl Lukas , Lipka Alexandra , Preusser Matthias , Marik Wolfgang , Bogner Wolfgang , Widhalm Georg , Rössler Karl , Trattnig Siegfried , Hangel Gilbert
Published: February 2024

This paper investigated the correlation between magnetic resonance spectroscopic imaging (MRSI) and magnetic resonance fingerprinting (MRF) in glioma patients by comparing neuro-oncological markers obtained from MRSI to T1/T2 maps from MRF. Data from 12 consenting patients with gliomas were analyzed by defining hotspots for T1, T2, and various metabolic ratios, and comparing them using Sørensen–Dice similarity coefficients (DSCs) and the distances between their centers of intensity (COIDs). The median DSCs between MRF and the tumor segmentation were 0.73 (T1) and 0.79 (T2). The DSCs between MRSI and MRF were the highest for Gln/tNAA (T1: 0.75, T2: 0.80, tumor: 0.78), followed by Gly/tNAA (T1: 0.57, T2: 0.62, tumor: 0.54) and tCho/tNAA (T1: 0.61, T2: 0.58, tumor: 0.45). The median values in the tumor hotspot were T1 = 1724 ms, T2 = 86 ms, Gln/tNAA = 0.61, Gly/tNAA = 0.28, Ins/tNAA = 1.15, and tCho/tNAA = 0.48, and, in the peritumoral region, were T1 = 1756 ms, T2 = 102 ms, Gln/tNAA = 0.38, Gly/tNAA = 0.20, Ins/tNAA = 1.06, and tCho/tNAA = 0.38, and, in the NAWM, were T1 = 950 ms, T2 = 43 ms, Gln/tNAA = 0.16, Gly/tNAA = 0.07, Ins/tNAA = 0.54, and tCho/tNAA = 0.20. The results of this study constitute the first comparison of 7T MRSI and 3T MRF, showing a good correspondence between these methods.
Neural Circuitry Polarization in the Spinal Dorsal Horn (SDH): A Novel Form of Dysregulated Circuitry Plasticity during Pain Pathogenesis
Authors: Chen Xufeng , Tang Shao-Jun
Published: February 2024

Pathological pain emerges from nociceptive system dysfunction, resulting in heightened pain circuit activity. Various forms of circuitry plasticity, such as central sensitization, synaptic plasticity, homeostatic plasticity, and excitation/inhibition balance, contribute to the malfunction of neural circuits during pain pathogenesis. Recently, a new form of plasticity in the spinal dorsal horn (SDH), named neural circuit polarization (NCP), was discovered in pain models induced by HIV-1 gp120 and chronic morphine administration. NCP manifests as an increase in excitatory postsynaptic currents (EPSCs) in excitatory neurons and a decrease in EPSCs in inhibitory neurons, presumably facilitating hyperactivation of pain circuits. The expression of NCP is associated with astrogliosis. Ablation of reactive astrocytes or suppression of astrogliosis blocks NCP and, concomitantly, the development of gp120- or morphine-induced pain. In this review, we aim to compare and integrate NCP with other forms of plasticity in pain circuits to improve the understanding of the pathogenic contribution of NCP and its cooperation with other forms of circuitry plasticity during the development of pathological pain.
In Silico Approach to Molecular Profiling of the Transition from Ovarian Epithelial Cells to Low-Grade Serous Ovarian Tumors for Targeted Therapeutic Insights
Authors: Leblebici Asim , Sancar Ceren , Tercan Bahar , Isik Zerrin , Arayici Mehmet Emin , Ellidokuz Ender Berat , Basbinar Yasemin , Yildirim Nuri
Published: February 2024

This paper aims to elucidate the differentially coexpressed genes, their potential mechanisms, and possible drug targets in low-grade invasive serous ovarian carcinoma (LGSC) in terms of the biologic continuity of normal, borderline, and malignant LGSC. We performed a bioinformatics analysis, integrating datasets generated using the GPL570 platform from different studies from the GEO database to identify changes in this transition, gene expression, drug targets, and their relationships with tumor microenvironmental characteristics. In the transition from ovarian epithelial cells to the serous borderline, the FGFR3 gene in the “Estrogen Response Late” pathway, the ITGB2 gene in the “Cell Adhesion Molecule”, the CD74 gene in the “Regulation of Cell Migration”, and the IGF1 gene in the “Xenobiotic Metabolism” pathway were upregulated in the transition from borderline to LGSC. The ERBB4 gene in “Proteoglycan in Cancer”, the AR gene in “Pathways in Cancer” and “Estrogen Response Early” pathways, were upregulated in the transition from ovarian epithelial cells to LGSC. In addition, SPP1 and ITGB2 genes were correlated with macrophage infiltration in the LGSC group. This research provides a valuable framework for the development of personalized therapeutic approaches in the context of LGSC, with the aim of improving patient outcomes and quality of life. Furthermore, the main goal of the current study is a preliminary study designed to generate in silico inferences, and it is also important to note that subsequent in vitro and in vivo studies will be necessary to confirm the results before considering these results as fully reliable.
Porous and Dense Alginate/Chitosan Composite Films Loaded with Simvastatin for Dressing Applications
Authors: Monteiro Rubens T. , Da Silva Thamyres F. , de Souza Guedes Luciana , Moreira Filho Raimundo N. F. , Soares Ana L. B. , Vasconcelos Niédja F. , Andrade Fabia K. , Vieira Rodrigo S.
Published: February 2024

Alginate is a biocompatible polysaccharide matrix used for bioactive dressings with inherent healing properties. Most alginate dressings are produced as single-layer dressings. This study explores the potential of bilayer membranes to modulate drug release and enhance antimicrobial properties. We used alginate and chitosan loaded with simvastatin, an anti-inflammatory drug. One membrane comprised dense layers of both alginate and chitosan, while the other featured a dense alginate upper layer and a porous chitosan lower layer. The current study introduces a new approach in which a bilayer membrane is modeled instead of creating a polymeric blend between alginate and chitosan. The upper layer of the membrane contains only alginate loaded with simvastatin, while the bottom layer contains only chitosan. Another innovation is the study of the use of a porous lower layer of chitosan. Therefore, the association of these polymers in a bilayer and porous membrane gives advanced therapeutic dressings (with anti-inflammatory and antimicrobial properties intrinsic to the membrane) that are more efficient in the healing of complex wounds. Comprehensive characterization encompassed physicochemical, thermal, morphological, and mechanical properties. Microbiological tests were conducted using chitosan extract, and cytotoxicity evaluations were performed on fibroblast and keratinocyte cells. The results showed interlayer adhesion due to ionic interactions between alginate and chitosan surfaces. The drying process influenced the morphological and physicochemical features of the membranes. Simvastatin release profiles demonstrated sustained release over an extended period (approximately 60%–70% of the drug after 96 h). Storage assessments revealed that after six months, the membranes maintained around 98% of the initial simvastatin content. The antimicrobial activity test underscored the bacteriostatic efficacy of the chitosan porous layer, making it well-suited for infected wounds. Cell viability tests confirmed the non-cytotoxic nature of the films, highlighting their promising characteristics for treating diverse skin lesion types.
Effect of Cl− on Passivation Properties of Fe-20Cr-20Mn-0.75N High Nitrogen Austenitic Stainless Steel
Authors: Zhang Wentao , Gao Fengyin , Zhou Huiling , Li Chengtao , Liu Zhong , Yang Haokun , Qiao Yanxin
Published: February 2024

In this work, Fe-20Cr-20Mn-0.75N (wt.%) high-nitrogen stainless steel (HNSS) was studied using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and electrochemical testing. The corrosion behaviors of Fe-20Cr-20Mn-0.75N HNSS with different concentrations of NaCl were studied. The composition of a passive film on Fe-20Cr-20Mn-0.75N HNSS was analyzed using X-ray photoelectron spectroscopy (XPS) at an applied potential of 0.2VSCE. The results showed that, with the increase in Cl− concentration, the corrosion tendency and corrosion rate of Fe-20Cr-20Mn-0.75N HNSS get higher. In the solution of a low Cl− concentration, the fraction of Fe and Cr oxides in the passive film is higher, and the passive film is thicker and more stable. By increasing the stability of the passive film and preventing its rupture, the elevated NH4+ concentration can enhance the corrosion resistance of Fe-20Cr-20Mn-0.75N HNSS in a NaCl solution.