Neural network modeling of fractal selforganization of microbial-organismic biosystems in the intestines of birds
Authors:   Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia, Kochish Ivan I. , Nikonov Ilya N. ,   Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia, Selina Marina V. ,   Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia, Vorobyov Nikolay I. ,   All-Russian Research Institute of Agricultural Microbiology, St. Petersburg, Russia
Published: January 2023

The purpose of this study was to determine feed probiotics, including Lactobacillus plantarum and Lactobacillus fermentum bacteria, which stimulate the bioconsolidation of microorganisms in the intestines of broilers, provide high efficiency of biochemical transformations of plant substrates in the intestines of birds and promptly supply the body of birds with all the necessary nutrients. To control the bio-consolidation (IndBcon) of the microbial community in the intestines of birds, it is proposed to calculate the bioconsolidation index according to the frequency-taxonomic profiles of operational taxonomic units (OTE) of microbial communities determined by the molecular genetic method. It is assumed that the high values of IndBcon correspond to the increased efficiency of biochemical processes of decomposition of fiber in the intestines of birds and the full development of birds. To determine the values of the IndBcon index, we selected a model fractal OTE profile in which the OTE frequencies are a log-arithmetic series of decreasing numbers (for example: log2 (0,125) = –3; log2 (0,25) = –2; log2 (0,5) = –1; ...). This makes it possible to calculate the IndBcon index based on the deviation of the real OTE profiles from the model fractal OTE profile, and by comparing the IndBcon values for different bird diets, determine the diet that ensures the bioconsolidation of the intestinal microflora. An artificial computational neural fractal network (NFS) was developed to analyze the profiles of the OTE microflora of the intestines of birds and calculate the IndBcon indices. The NSF can simultaneously process several OTE profiles (for all variants of the experiment) and calculate the IndBcon values for each variant, that is, to obtain a quantitative assessment of the impact of the diet of birds on the biconsolidation of the microbial community in the intestines of birds. An artificial computational neural fractal network (NFS) was developed to analyze the profiles of the OTE microflora of the intestines of birds and calculate the IndBcon indices. The NSF can simultaneously process several OTE profiles (for all variants of the experiment) and calculate the IndBcon values for each variant, that is, to obtain a quantitative assessment of the impact of the diet of birds on the biconsolidation of the microbial community in the intestines of birds. In this experimental and theoretical study, three OTE profiles obtained by molecular genetic method in one control group (group 1) and two experimental groups (groups 2 and 3) of birds were compared. A probiotic with the bacterium Lactobacillus plantarum was included in the feed diet of the 2nd group of birds, and a probiotic with the bacterium Lactobacillus fermentum was included in the feed diet of the 3rd group. Processing of three OTE profiles using NSF showed that in birds of group 1 IndBcon = 0,52, in birds of group 2 IndBcon = 0,24, in birds of group 3 IndBcon = 0,74. It turned out that these IndBcon values correlate with the masses of broilers in the corresponding groups (r=0,94), which confirms the influence of bioconsolidation of the microbial community of the intestines of birds on the activation of biochemical processes in the intestines of birds and their accelerated development of birds. Based on the study, it was recommended to use feed probiotics with Lactobacillus fermentum bacteria, since they help achieve the highest value of IndBcon and bioconsolidation of the intestinal microflora of birds.