bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimation and Influencing Factor Analysis of Carbon Emissions From the Entire Production Cycle for Household consumption: Evidence From the Urban Communities in Beijing, China
Authors: Wang Jiabin , Hui Wenjie , Liu Lian , Bai Yuping , Du Yudong , Li Jiajin
Published: April 2022

Household carbon emissions (HCEs) in urban communities are significant sources of China’s total carbon emissions and contribute to global warming and climate change dramatically. This study aims to estimate the HCEs and investigate their influential factors based on a total of 185 household survey data collected from three typical types of urban communities in Beijing: traditional communities, unit communities, and commercial housing communities with the application of the consumer lifestyle approach analysis and econometrics model. The results show that unit communities contribute to the highest direct carbon emissions and the commercial housing communities produce the most indirect carbon emissions, with the traditional communities emitting the lowest carbon emissions both directly and indirectly. The highest direct carbon emissions of households are found in unit communities at 723.79 kgCO2 per month, followed by commercial communities at 580.01 kgCO2, and finally the traditional communities with 526.44 kgCO2 direct carbon emissions monthly. And the highest monthly indirect carbon emissions of households are found in commercial communities at 707.70 kgCO2, followed by unit communities at 669.38 kgCO2, and finally with 554.85 kgCO2 indirect carbon emissions monthly in traditional communities. It concludes that the community type affects HCE characteristics and their driving factors significantly. Household income, household population, and the ownership of cars increase HCE in more than one type of community. Scientific research work-related population, community environment satisfaction, housing area have positive effects, while community convenience has negative impacts on HCEs in one certain type of community. Policy implications tailored to general and specific community types are proposed as the guidance of carbon reduction and community transformation finally. This study contributes to the understanding of the impact of community attributes on HCEs and proposes some methods for microregional carbon emission reduction and the ecological transformation of urban communities.
Construction of GI Network Based on MSPA and PLUS Model in the Main Urban Area of Zhengzhou: A Case Study
Authors: Zhang Jinbing , Zhu Huiru , Zhang Pengyan , Song Yongpeng , Zhang Ying , Li Yanyan , Rong Tianqi , Liu Zhenyue , Yang Dan , Lou Yuanyuan
Published: April 2022

With continuous urbanization and the fragmentation of green areas that affect human well-being, the establishment of a green infrastructure (GI) network is important in future urban planning. As a National Central City, Zhengzhou has a large population and is undergoing rapid economic development, resulting in an urgent demand for green space within the city in recent years. We selected the main urban area of Zhengzhou as the study area based on the two phases of Landsat 8 satellite remote sensing image data, for 2016 and 2021, and used the patch-generating land use simulation (PLUS) model to predict the spatial distribution of GI in the future; compared with traditional methods, this method identified green spaces from a future perspective. A GI network—consisting of an open space with vegetation as the main body—was designed for the main urban area of Zhengzhou using the traditional landscape ecological pattern theory, integrating morphological spatial pattern analysis (MSPA), the minimum cumulative resistance (MCR) model, circuit theory, and other methods. Evidently, the area of green space in Zhengzhou City in 2021 was 36 231.6 hm2. GI prediction results indicate that continuous expansion of the main urban area did not result in significant changes in the size of GI in the city. The GI within the urban area was relatively fragmented, forming 15 GI hubs, most of which were densely distributed along the edges of the main urban area. This study proposed the construction of a GI network with a target corridor and target points based on the existing corridor. This included the identification of seven target corridors and 15 target hubs; the total length of the corridor was 77.032 km, with a total of 31 target points. In summary, the GI network pattern of “one protection barrier, two lines, three loops and more points” was proposed. With new urban problems constantly emerging, this research could provide a theoretical reference basis for the planning of GI in the main urban area of the National Central City. The study provides concrete evidence on the optimum pattern for the construction of GI networks in cities with large populations.
Factors Controlling the Change of Soil Erosion Intensity in Mountain Watersheds in Serbia
Authors: Manojlović Sanja , Sibinović Mikica , Srejić Tanja , Novković Ivan , Milošević Marko V. , Gatarić Dragica , Carević Ivana , Batoćanin Natalija
Published: April 2022

The intensity of soil erosion is the result of a combined action of natural factors and different human activities. This work aims to determine the factors controlling the change of soil erosion. Eleven watersheds from different parts of Serbia were used as the study area. An Erosion Potential Model was applied to estimate the soil erosion status of the watersheds in two periods, 1971 and 2010. The model indicated that the reduction of soil erosion intensity in the watersheds ranges from 12.4% to 82.7%. The statistical analysis examines quantitative relationships and combined effects between soil erosion and socio-economic and main physical-geographical determinants in watersheds. Watershed characteristics were divided into 5 classes, and within each class 22 variables were calculated: two variables relate to erosion, one to topography, two to land cover, seven to demographic and ten to agrarian variables. Correlation analysis and Principal Component Analysis (PCA) have been applied to understand the main variables that contribute to change soil erosion intensity. The PCA identified four components that can explain at least up to 79.06% of the variation of all variables. This study explores new indicators for correlations with changing soil erosion and provides decision makers with access to quantification for environmental impact assessment and decision-making for adequate soil conservation and management programs.
Manipulation of Heat Dissipation Capacity Affects Avian Reproductive Performance and Output
Authors: Zagkle Elisavet , Martinez-Vidal Paola Alexandra , Bauchinger Ulf , Sadowska Edyta T.
Published: April 2022

Animal life requires hard work but the ability to endure such workload appears to be limited. Heat dissipation limit (HDL) hypothesis proposes that the capacity to dissipate the excess of body heat during hard work may limit sustained energy use. Experimental facilitations of heat loss rate via feather-clipping in free-living birds seem to support HDL hypothesis but testing of HDL through laboratory experiments under controlled conditions is not reported. We employed a two-factorial experimental design to test HDL hypothesis by manipulating the capacity to dissipate heat through exposure of captive zebra finches (Taeniopygia guttata) to a cold and warm ambient temperature (14°C and 25°C), and through manipulation of the insulating layer of feathers around the brood patch in females (clipped and unclipped). To simulate foraging costs encountered in the wild we induced foraging effort by employing a feeding system that necessitated hovering to access food, which increased energetic costs of reproduction despitead libitumconditions in captivity. We quantified the outcome of reproductive performance at the level of both parents, females, and offspring. Thermal limitations due to warm temperature appeared at the beginning of reproduction for both parents with lower egg-laying success, smaller clutch size and lower egg mass, compared to the cold. After hatching, females with an enhanced ability to dissipate heat through feather-clipping revealed higher body mass compared to unclipped females, and these clipped females also raised heavier and bigger nestlings. Higher levels for oxidative stress in plasma of females were detected prior to reproduction in warm conditions than in the cold. However, oxidative stress biomarkers of mothers were neither affected by temperature nor by feather-clipping during the reproductive activities. We document upregulation of antioxidant capacity during reproduction that seems to prevent increased levels of oxidative stress possibly due to the cost of female body condition and offspring growth. Our study on reproduction under laboratory-controlled conditions corroborates evidence in line with the HDL hypothesis. The link between temperature-constrained sustained performance and reproductive output in terms of quality and quantity is of particular interest in light of the current climate change, and illustrates the emerging risks to avian populations.
Beyond Climatic Variation: Human Disturbances Alter the Effectiveness of a Protected Area to Reduce Fires in a Tropical Peatland
Authors: Imron Muhammad Ali , Widyastuti Kirana , Al Bihad Dennis , Satria Ryan Adi , Prayoga Wiwid , Pradopo Subyantoro Tri , Suryatmojo Hatma , Sopha Bertha Maya , Harrison Mark E. , Berger Uta
Published: April 2022

Fire is considered a major threat to biodiversity in many habitats and the occurrence of fire has frequently been used to investigate the effectiveness of protected areas. Yet, despite the known importance of tropical peatlands for biodiversity conservation and serious threat that anthropogenically induced fires pose to this ecosystem, the influence of protected area designation on fire occurrence in tropical peatland has been poorly assessed thus far. Our study addresses this knowledge gap through providing a novel assessment of fire patterns from a tropical peatland protected area and surrounding landscape. We investigated the importance of both climatic factors (top-down mechanism) and human interventions (bottom-up mechanism) on fire occurrence through analyzing 20-years (2001–2020) of LANDSAT and Moderate Resolution Imaging Spectrometer (MODIS) images of the Padang Sugihan Wildlife Reserve and a 10-km buffer area surrounding this in Sumatra, Indonesia. Fire density was assessed in relation to road and canal construction. Monthly and annual precipitation was compared between wet and dry years. The reserve was effective in limiting fire compared to surrounding landscapes only in wet years. We revealed that peat fire occurrence in the protected area and buffer zone was not due to climatic factors alone, with distance from canals and roads also contributing toward fire occurrence. Our results suggest that it is essential to address tropical peatland fire processes at a landscape level, particularly at the surroundings of protected areas, in order to increase the effectiveness of fire protection, improve fire risk classification maps, and conserve threatened tropical peatland wildlife such as the Sumatran elephant.
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection
Authors: Pimenta de Paiva Luciene , Coelho-dos-Reis Jordana Grazziela Alves , Trindade Bruno Caetano , Peruhype-Magalhães Vanessa , Silva Araújo Márcio Sobreira , Gonçalves Juan Jonathan , Nogueira-Souza Ana Caroline , Pereira Martins Júlia , Lopes Ribeiro Ágata , Starling Ana Lucia , Alcântara Luiz Carlos Júnior , Ribeiro Maísa Aparecida , Carneiro-Proietti Anna Bárbara de Freitas , Sabino Ester Cerdeira , Alves Bicalho Kelly , Teixeira-Carvalho Andréa , Martins-Filho Olindo Assis
Published: April 2022

In the present work, we developed and evaluated the performance of a new flow cytometry-based single platform, referred to as “FC-Duplex IgG1 (HTLV-1/2)”, for universal and differential serodiagnosis of HTLV-1/2 infection. The proposed technology employs a system for detection of IgG1 antibodies in a single competitive immunofluorescence platform by flow cytometry using fluorescently labeled MT-2/MoT cell line mix coupled to a highly sensitive development system (Biotin/Streptavidin/Phycoerythrin). The stability of fluorescent labeling and the antigenicity of MT-2 and MoT cell lines were confirmed upon storage at −20°C for 2, 6, and 12 months. The anti-HTLV-1/2 IgG1 reactivity, expressed as percentage of positive fluorescent cells (PPFC), was evaluated for each target antigen along the titration curve of test serum samples (1:32 to 1:4,096). Upon selection of target cell line and serum dilutions with higher segregation score between groups, the performance of “FIX” and “FIX & PERM” protocols was evaluated. The “FIX” protocol presented excellent performance indices (Se = 92%/Sp = 94%/AUC = 0.96; Se = 96%/Sp = 100%/AUC = 0.99) for the universal (HTLV-1/2 vs. NI) and differential (HTLV-1 vs. HTLV-2) diagnosis of HTLV-1 infection, respectively. Optimization of the “FIX” protocol using the principle of synchronous and asynchronous pairwise analysis further improved the performance of “FC-Duplex IgG1 (HTLV-1/2)”, using the “FIX” protocol for differential diagnosis of HTLV-1 and HTLV-2 infections (Se = 100%/Sp = 100%/AUC = 1.00). In conclusion, the “FC-Duplex IgG1 (HTLV-1/2)” method represents an innovation in the biotechnology segment with the potential to compose a serological kit for differential diagnosis of HTLV-1/2 infection for reference laboratories and blood centers.
Heterophil/Lymphocyte Ratio Level Modulates Salmonella Resistance, Cecal Microbiota Composition and Functional Capacity in Infected Chicken
Authors: Thiam Mamadou , Wang Qiao , Barreto Sánchez Astrid Lissette , Zhang Jin , Ding Jiqiang , Wang Hailong , Zhang Qi , Zhang Na , Wang Jie , Li Qinghe , Wen Jie , Zhao Guiping
Published: April 2022

The gastrointestinal microbiota plays a vital role in ensuring the maintenance of host health through interactions with the immune system. The Heterophil/Lymphocyte (H/L) ratio reflects poultry’s robustness and immune system status. Chickens with low H/L ratio are superior to the chickens with high H/L ratio in survival, immune response, and resistance to Salmonella infection, but the underlying mechanisms remain unclear. This study aimed to identify microorganisms associated with resistance to Salmonella Enteritidis infection in chickens based on the H/L ratio. The 16S rRNA and metagenomic analysis were conducted to examine microbiome and functional capacity between the 2 groups, and Short Chain Fatty Acids (SCFAs) and histopathology were conducted to explore the potential difference between susceptible and resistant groups at 7 and 21 days post-infection (dpi). The microbiome exploration revealed that low H/L ratio chickens, compared to high H/L ratio chickens, displayed a significantly higher abundance of Proteobacteria (Escherichia coli) and Bacteroidetes (Bacteroides plebeius) at 7 and 21 dpi, respectively. Anaerostipes (r = 0.63) and Lachnoclostridium (r = 0.63) were identified as bacterial genus significantly correlated with H/L (P < 0.001). Interestingly, Bacteroides was significantly and positively correlated with bodyweight post-infection (r = 0.72), propionate (r = 0.78) and valerate (r = 0.82) contents, while Salmonella was significantly and negatively correlated with bodyweight post-infection (r = − 0.67), propionate (r = − 0.61) and valerate (r = − 0.65) contents (P < 0.001). Furthermore, the comparative analysis of the functional capacity of cecal microbiota of the chickens with high and low H/L ratio revealed that the chickens with low H/L ratio possess more enriched immune pathways, lower antibiotic resistance genes and virulence factors compared to the chickens with high H/L ratio. These results suggest that the chickens with low H/L ratio are more resistant to Salmonella Enteritidis, and it is possible that the commensal Proteobacteria and Bacteroidetes are involved in this resistance against Salmonella infection. These findings provide valuable resources for selecting and breeding disease-resistant chickens.
Case Report: A Case With MOGAD and Anti-NMDAR Encephalitis Overlapping Syndrome Mimicing Radiological Characteristics of CLIPPERS
Authors: Guo Jia , Bu Yujie , Liu Wei
Published: April 2022

Herein, we reported a case of a young man diagnosed with MNOS (anti-myelin oligodendrocyte glycoprotein associated disease (MOGAD) and anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis overlapping syndrome, i.e., MNOS), whose imaging findings in magnetic resonance imaging (MRI) mimicked chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids (CLIPPERS). We reported a case of refractory anti-NMDAR encephalitis that recurred after standard first-line and second-line treatment. The patient presented with CLIPPERS on imaging at recent hospital admission, and his MOG antibodies were seropositive. After intravenous methylprednisolone (IVMP) treatment, the patient’s symptoms were significantly alleviated. In this case, we demonstrated that MNOS could mimic the radiological characteristics of CLIPPERS. Future studies should focus on the diagnosis and treatment of antibody overlap syndrome.
Protein Arginine Methylation: An Emerging Modification in Cancer Immunity and Immunotherapy
Authors: Dai Weijing , Zhang Jianguo , Li Siqi , He Fajian , Liu Qiao , Gong Jun , Yang Zetian , Gong Yan , Tang Fang , Wang Zhihao , Xie Conghua
Published: April 2022

In recent years, protein arginine methyltransferases (PRMTs) have emerged as new members of a gene expression regulator family in eukaryotes, and are associated with cancer pathogenesis and progression. Cancer immunotherapy has significantly improved cancer treatment in terms of overall survival and quality of life. Protein arginine methylation is an epigenetic modification function not only in transcription, RNA processing, and signal transduction cascades, but also in many cancer-immunity cycle processes. Arginine methylation is involved in the activation of anti-cancer immunity and the regulation of immunotherapy efficacy. In this review, we summarize the most up-to-date information on regulatory molecular mechanisms and different underlying arginine methylation signaling pathways in innate and adaptive immune responses during cancer. We also outline the potential of PRMT-inhibitors as effective combinatorial treatments with immunotherapy.
The Role of Satellite Telemetry Data in 21st Century Conservation of Polar Bears (Ursus maritimus)
Authors: Laidre Kristin L. , Durner George M. , Lunn Nicholas J. , Regehr Eric V. , Atwood Todd C. , Rode Karyn D. , Aars Jon , Routti Heli , Wiig Øystein , Dyck Markus , Richardson Evan S. , Atkinson Stephen , Belikov Stanislav , Stirling Ian
Published: April 2022

Satellite telemetry (ST) has played a critical role in the management and conservation of polar bears (Ursus maritimus) over the last 50 years. ST data provide biological information relevant to subpopulation delineation, movements, habitat use, maternal denning, health, human-bear interactions, and accurate estimates of vital rates and abundance. Given that polar bears are distributed at low densities over vast and remote habitats, much of the information provided by ST data cannot be collected by other means. Obtaining ST data for polar bears requires chemical immobilization and application of a tracking device. Although immobilization has not been found to have negative effects beyond a several-day reduction in activity, over the last few decades opposition to immobilization and deployment of satellite-linked radio collars has resulted in a lack of current ST data in many of the 19 recognized polar bear subpopulations. Here, we review the uses of ST data for polar bears and evaluate its role in addressing 21st century conservation and management challenges, which include estimation of sustainable harvest rates, understanding the impacts of climate warming, delineating critical habitat, and assessing potential anthropogenic impacts from tourism, resource development and extraction. We found that in subpopulations where ST data have been consistently collected, information was available to estimate vital rates and subpopulation density, document the effects of sea-ice loss, and inform management related to subsistence harvest and regulatory requirements. In contrast, a lack of ST data in some subpopulations resulted in increased bias and uncertainty in ecological and demographic parameters, which has a range of negative consequences. As sea-ice loss due to climate warming continues, there is a greater need to monitor polar bear distribution, habitat use, abundance, and subpopulation connectivity. We conclude that continued collection of ST data will be critically important for polar bear management and conservation in the 21st century and that the benefits of immobilizing small numbers of individual polar bears in order to deploy ST devices significantly outweigh the risks.
Label-Free Quantitative Proteomic Analysis Provides Insight Into the Differences Between Slow-Twitch Muscle and Fast-Twitch Muscle of Pseudocaranx dentex
Authors: Wang Huan , Li Busu , Li Jiefeng , Jiang Chen , Liu Shufang , Zhuang Zhimeng
Published: April 2022

Fish skeletal muscles are mainly composed of two distinct types, fast-twitch and slow-twitch muscles, and they play important roles in maintaining movement and energy metabolism. The fast-twitch muscle contracts quickly and is mainly responsible for burst swimming, while the slow-twitch muscle possesses fatigue resistance and supports endurance swimming. To assess the differences in molecular composition and investigate the potential regulatory mechanisms, we performed an integrative study at both proteomic and transcriptomic levels of the fast-twitch and slow-twitch muscles in Pseudocaranx dentex, a pelagic migratory fish with distinctly differentiated skeletal muscle. Label-free proteomics revealed 471 differentially expressed proteins (DEPs), 422 upregulated and 49 downregulated in slow-twitch muscle when compared to fast-twitch muscle. These DEPs were mainly involved in myofibrillary structure and energy metabolism. Integrative analysis of proteomic and transcriptomic data showed that 757 RNA-protein pairs were positively correlated, and 191 RNA-protein pairs were negatively correlated in abundance. Meanwhile, 311 RNA-protein pairs were consistent in fold changes, and 594 RNA-protein pairs exhibited striking differences, which provided an insight into the complex regulation at both transcriptional and post-transcriptional levels that contribute to shaping the different muscle types. The specific expression of multiple myofibrillar proteins, such as myosin, actin, troponin, and tropomyosin, suggested that the distinction in contraction characterizations between slow-twitch and fast-twitch muscles is related to different protein isoforms. Muscle-type specific expression of gene-encoding key enzymes in fatty acid metabolism, glycolysis, tricarboxylic acid (TCA) cycle, and oxidative phosphorylation pathways, such as carnitine palmitoyltransferase (CPT2), phosphofructokinase (Pfkm), pyruvate kinase (Pkmb), citrate synthase (CS), isocitrate dehydrogenase (IDH), and 2-oxoglutarate dehydrogenase complex (Ogdh), may be the molecular basis responsible for the differences in energy metabolism. Overall, this global view of protein and RNA expression levels in P. dentex fast-twitch and slow-twitch muscles reveals the essential roles of transcriptional and post-transcriptional regulation in maintaining muscle structure and function. The identified potential genes that may cause the differences in physiological characteristics will greatly improve our understanding on the molecular basis of skeletal muscle contraction, metabolism, and regulation in teleost.
Interannual Variability of Yellowfin Tuna (Thunnus albacares) and Bigeye Tuna (Thunnus obesus) Catches in the Southwestern Tropical Indian Ocean and Its Relationship to Climate Variability
Authors: Kim Jihwan , Na Hanna
Published: April 2022

This study investigated the interannual variability of yellowfin tuna (Thunnus albacares) and bigeye tuna (Thunnus obesus) catches in the southwestern tropical Indian Ocean (SWTIO) over 25 years and its relationship to climate variability. The results indicate that the catch amount in the northern SWTIO exhibits a significant relationship with the temperature, salinity, and current variability in the upper ocean (< 400 m), associated with a significant subsurface upwelling variability, which is prominent only in the northern region. An increase of the tuna catches in the northern region is associated with the deepening of the thermocline depth and 20°C isotherm depth of the Seychelles–Chagos Thermocline Ridge, indicating suppression of the subsurface upwelling. Further analysis reveals that the catch amounts in the SWTIO tend to increase during the positive phase of the Indian Ocean Dipole. However, the catch variability in the northern SWTIO is more closely related to the El Niño–Southern Oscillation than the Indian Ocean Dipole. Favorable conditions for catches seem to develop in the northern region during El Niño years and continue throughout the following years. This relationship suggests the potential predictability of catch amounts in the northern SWTIO, an energetic region with strong subsurface upwelling variability.
Environmental Evolution and Human Adaption Recorded From a Salt Production Site at the Coastal Plain of Laizhou Bay, China
Authors: Guo Yuanyuan , Mao Longjiang , Zhu Likai , Mo Duowen
Published: April 2022

The southern coast of the Laizhou Bay is considered as one of important areas for the origin and development of sea salt production in Eastern China. Archaeologists have collected rich materials to better understand history of salt production in the region, but how environmental change influences early salt production is still unknown. Here we collected samples at the Shuangwangcheng (SWC) site in the southern plain of Laizhou Bay. We conducted grain size, mollusk and foraminifera analysis to examine the evolution of sedimentary environment and restored the human adaptions to environmental change. The results showed that the sedimentary environment of study area was lower tidal flat during 6400~5900 yr BP as indicated by coarse and well sorted grain size, high abundance of foraminifera, and the Ammonia beccarii vars. - Quinqueloculina akneriana foraminifera assemblage. Sedimentary environment changed from intertidal to supratidal flat with decreasing abundance of foraminifera and finer upward grain size at 5900~ 4300 yr BP. After 4300 yr BP, this area was less affected by seawater, which could be reflected by the appearance of freshwater mollusk and rarely discovered foraminifera. Our retrieved environment changes were closely related with ancient human activities. The Holocene transgression constrained the Dawenkou cultural sites within the inland areas with higher altitude. The exposed coast lowlands after sea retreat were initially not suitable for human survival except sporadic salt production sites of the Longshan culture. These conditions were improved during the Shang and Zhou Dynasties when humans widely used the particular natural resources of underground brine for salt production, and then a large number of salt production sites appeared, which made this region develop into an important origin center of salt production. Our research suggests that salt production was an economic activity that was adopted by people to adapt to the harsh environment, which is of great significance for understanding the evolution of the human-environmental relationship in the coastal area.
Sectional Analysis of Reinforced Engineered Cementitious Composite Columns Subjected to Combined Lateral Load and Axial Compression
Authors: Wu Chang , Su Yanli , Sun Yu , Jin Chenhua , Pan Zuanfeng
Published: April 2022

The ultra-high tensile ductility of ECC provides an alternative way to enhance the ductility of structural members by using high-ductile matrix material instead of simply increasing reinforcements. However, the application of ECC members is still limited due to the relatively short research time and the lack of design specifications. Being equivalent to eccentric compressive members, a sectional analysis of RECC columns subjected to combined lateral load and axial compression are proposed in this paper. Based on the design theory of load capacity of eccentric compression columns and the unique constitutive model of ECC, the calculation equations for the sectional load capacity of RECC columns are derived. The analytical prediction of the load capacity of RECC column is evaluated in comparison with that of experiments that confirm the capacity of the proposed calculation method to capture the behavior of the RECC column accurately. The strength-interaction diagrams showing the axial force-moment (N-M) interaction curves are then constructed for analysis using the proposed calculation equations. A parametric study is also carried out by using the proposed calculation equations, demonstrating the effects of ultimate tensile strain of ECC, compressive and tensile strength of ECC, yield strength of steel bar, and reinforcement ratio on the N-M interaction curves of RECC columns. The investigations exhibited in this paper are expected to provide insight into the design principles of RECC columns.
Literature Review on the Discrete Element Method in Asphalt Mixtures
Authors: Yao Hui , Xu Mei , Liu Junfu , Liu Yu , Ji Jie , You Zhanping
Published: April 2022

Asphalt mixtures are commonly used in pavement engineering, especially for highway construction. The mechanism exploration and analysis of pavement distress are the main challenges for researchers and industry managers. Thereby, it is important to understand their properties and interaction mechanisms in asphalt mixtures. It is difficult to conduct some sophisticated or microscale tests in the laboratory, and numerical simulation and virtual tests can be solutions for these cases with low costs. The Discrete Element Method (DEM) is a promising tool for researchers to undertake these tasks. This paper mainly summarized and analyzed the research progress and development prospect of DEMs in asphalt mixtures from a series of technical sections. The laboratory test results were often used to calibrate the DEM simulations as well as Two-dimensional (2D) and Three-dimensional (3D) modeling. Several modeling methods were developed to generate digital samples, like user-defined, image-based, random-modeling. In addition, the conclusions can be referenced by researchers for the development of numerical simulations.
Microbiology and Outcome of Peritoneal Dialysis-Related Peritonitis in Elderly Patients: A Retrospective Study in China
Authors: Song Panai , Yang Dong , Li Jine , Zhuo Ning , Fu Xiao , Zhang Lei , Zhang Hongqing , Liu Hong , Sun Lin , Liu Yinghong
Published: April 2022

Objective

The number of elderly patients on peritoneal dialysis (PD) has rapidly increased in the past few decades. We sought to explore the microbiology and outcomes of peritonitis in elderly PD patients compared with younger PD patients.

Methods

We conducted a retrospective study to analyze the clinical characteristics, causative organism distribution, and outcome of all PD patients who developed peritonitis between September 1, 2014 and December 31, 2020, from Second Xiangya Hospital, Central South University, China. Patients who experienced peritonitis were separated into elderly and younger groups. The elderly was defined as ≥ 65 years old at the initiation of PD.

Results

Among 1,200 patients, 64(33.9%) in elderly (n = 189) and 215 (21.3%) in younger (n = 1,011) developed at least one episode of peritonitis. A total of 394 episodes of peritonitis occurred in 279 patients. Of these, 88 episodes occurred in 64 elderly patients, and 306 episodes occurred in 215 younger patients. Gram-positive bacteria were the main causative organisms in elderly and younger patients (43.2% and 38.0%, respectively). Staphylococcus and Escherichia coli were the most common gram-positive and gram-negative bacteria, respectively. Fungal peritonitis in elderly patients was higher compared with younger patients (χ2 = 6.55, P = 0.01). Moreover, Acinetobacter baumannii (χ 2=9.25, P = 0.002) and polymicrobial peritonitis (χ 2 = 6.41, P = 0.01) in elderly patients were also significantly higher than that in younger patients. Additionally, elderly PD patients had higher peritonitis-related mortality than younger patients (χ 2 = 12.521, P = 0.000), though there was no significant difference in catheter removal between the two groups. Kaplan-Meier analysis showed that cumulative survival was significantly lower in elderly patients than younger patients (log rank = 7.867, p = 0.005), but similar technical survival in both groups (log rank = 0.036, p = 0.849).

Conclusions

This retrospective study demonstrated that elderly PD patients were more likely to develop Acinetobacter baumannii, fungal and polymicrobial peritonitis than younger PD patients. In addition, peritonitis-related mortality was significantly higher in elderly patients, whereas peritonitis-related catheter removal was comparable between elderly and younger PD patients. Understanding microbiology and outcome in elderly patients will help to reduce the incidence of PD-associated peritonitis and improve the quality of life.
Biannual and Quarterly Comparison Analysis of Agglutinating Antibody Kinetics on a Subcohort of Individuals Exposed to Leptospira interrogans in Salvador, Brazil
Authors: Cruz Jaqueline S. , Nery Nivison , Sacramento Gielson A. , Victoriano Renato , Montenegro Albino L. S. , Santana Juliet O. , Costa Federico , Ko Albert I. , Reis Mitermayer G. , Wunder Elsio A.
Published: April 2022

Introduction

Leptospirosis is a zoonosis with a worldwide spread that leads to clinical manifestations ranging from asymptomatic infection to a life-threatening disease. The immune response is predominantly humoral mediated limited to the infecting serovar. Individuals living in an area endemic for leptospirosis are often exposed to an environment contaminated with leptospires and there is a paucity of information on naturally acquired immunity. In the present study, we evaluated the kinetics of agglutinating antibodies in individuals from an endemic area for leptospirosis in Salvador, Brazil comparing two different intersample collection times.

Methods

Between 2017–2018, we carried out a biannual prospective cohort with 2,086 individuals living in an endemic area for leptospirosis in Salvador, Brazil. To compare agglutinating antibody kinetics using microscopic agglutination test (MAT) with different collection times, a subcohort of 72 individuals with quarterly follow-up was carried out in parallel.

Results

The results revealed that using a shorter time for intersample collection led to the detection of a higher number of infections and reinfection events. Furthermore, we observed a higher rate of titer decay indicating partial and short protection. However, there was no indication of major changes in risk factors for the disease.

Conclusions

We evaluated antibody kinetics among residents of an endemic area for leptospirosis comparing two sample collection times. The constant exposure to the contaminated environment increases the risk for leptospirosis infection with reinfection events being more common than expected. This indicates that the burden of leptospirosis might be underestimated by serological surveys, and further studies are necessary to better characterize the humoral response after infection.
Epithelial-Mesenchymal Interaction in Hair Regeneration and Skin Wound Healing
Authors: Mao Mei-Qi , Jing Jing , Miao Yu-Jie , Lv Zhong-Fa
Published: April 2022

Interactions between epithelial and mesenchymal cells influence hair follicles (HFs) during embryonic development and skin regeneration following injury. Exchanging soluble molecules, altering key pathways, and extracellular matrix signal transduction are all part of the interplay between epithelial and mesenchymal cells. In brief, the mesenchyme contains dermal papilla cells, while the hair matrix cells and outer root sheath represent the epithelial cells. This study summarizes typical epithelial–mesenchymal signaling molecules and extracellular components under the control of follicular stem cells, aiming to broaden our current understanding of epithelial–mesenchymal interaction mechanisms in HF regeneration and skin wound healing.
Combination of Vitamin C and Curcumin Safeguards Against Methotrexate-Induced Acute Liver Injury in Mice by Synergistic Antioxidant Effects
Authors: Hasan Khudhair Dhekra , Al-Gareeb Ali I. , Al-kuraishy Hayder M. , El-Kadem Aya H. , Elekhnawy Engy , Negm Walaa A. , Saber Sameh , Cavalu Simona , Tirla Adrian , Alotaibi Saqer S. , Batiha Gaber El-Saber
Published: April 2022

Methotrexate (MTX), an antineoplastic and immunosuppressive drug, widely used in the treatment of different types of cancers and the management of chronic inflammatory diseases. However, its use is associated with hepatotoxicity. Vitamin C (VC) and curcumin (CUR) exhibit anti-inflammatory and antioxidant effects. Thus, we aimed to investigate the potential hepatoprotective effects of VC and CUR pretreatment alone and in combination against MTX-induced hepatotoxicity. Albino mice were randomly divided into 7 groups: the control group, which received only normal saline; MTX group; VC group, pretreated with VC (100 or 200 mg/kg/day orally) for 10 days; CUR group, pretreated with CUR (10 or 20 mg/kg/day orally); and combination group, which received VC (100 mg/kg) and CUR (10 mg/kg). MTX was administered (20 mg/kg, intraperitoneally) to all the groups on the tenth day to induce hepatotoxicity. Forty eight hours after MTX administration, the mice were anesthetized. Blood samples were collected, the liver was removed for biochemical analysis, and a part of the tissue was preserved in formalin for histopathological analysis. The results indicated that pretreatment with a combination of VC and CUR induced a more significant decrease in the serum levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, and lactic dehydrogenase and a significant increase in the tissue level of superoxide dismutase and glutathione; furthermore, it induced a significant decrease in malondialdehyde levels and improvement in histopathological changes in the liver tissues, confirming the potential hepatoprotective effects of the combination therapy on MTX-induced liver injury. To conclude, MTX-induced hepatotoxicity is mediated by induction of oxidative stress as evident by increased lipid peroxidation and reduction of antioxidant enzyme activity. Pretreatment with VC, CUR or their combination reduces the MTX-induced hepatotoxicity by antioxidant and anti-inflammatory effects. However, the combined effect of VC and CUR provided a synergistic hepatoprotective effect that surpasses pretreatment with CUR alone but seems to be similar to that of VC 200 mg/kg/day. Therefore, VC and CUR combination or a large dose of VC could be effective against MTX-induced hepatotoxicity. In this regard, further studies are warranted to confirm the combined hepatoprotective effect of VC and CUR against MTX-induced hepatotoxicity.
Phacoemulsification Combined With Supra-Capsular and Scleral-Fixated Intraocular Lens Implantation in Microspherophakia: A Retrospective Comparative Study
Authors: Chen Ze-Xu , Zhao Zhen-Nan , Sun Yang , Jia Wan-Nan , Zheng Jia-Lei , Chen Jia-Hui , Chen Tian-Hui , Lan Li-Na , Jiang Yong-Xiang
Published: April 2022

Background

Microspherophakia (MSP) is a rare ocular condition, the lens surgery of which is complicated by both insufficient zonules and undersized capsule.

Methods

This study included MSP eyes managed with phacoemulsification combined with supra-capsular and scleral-fixated intraocular lens implantation (SCSF-IOL) and made the comparison with those treated by transscleral-fixated modified capsular tension ring and in-the-bag intraocular lens implantation (MCTR-IOL).

Results

A total of 20 MSP patients underwent SCSF-IOL, and 17 patients received MCTR-IOL. The postoperative best corrected visual acuity was significantly improved in both groups (P < 0.001), but no difference was found between the groups (P = 0.326). The IOL tilt was also comparable (P = 0.216). Prophylactic Nd:YAG laser posterior capsulotomy was performed 1 week to 1 month after the SCSF-IOL procedure. In the SCSF-IOL group, two eyes (10.00%) needed repeated laser treatment and one eye (5.00%) had a decentered capsule opening. Posterior capsule opacification was the most common complication (6, 35.29%) in the MCTR group. No IOL dislocation, secondary glaucoma, or retinal detachment was observed during follow-up.

Conclusions

SCSF-IOL is a viable option for managing MSP and is comparable with the MCTR-IOL. Nd:YAG laser posterior capsulotomy was necessary to prevent residual capsule complications after the SCSF-IOL procedure.
Diabetic Retinopathy Grading by Deep Graph Correlation Network on Retinal Images Without Manual Annotations
Authors: Zhang Guanghua , Sun Bin , Chen Zhixian , Gao Yuxi , Zhang Zhaoxia , Li Keran , Yang Weihua
Published: April 2022

Background

Diabetic retinopathy, as a severe public health problem associated with vision loss, should be diagnosed early using an accurate screening tool. While many previous deep learning models have been proposed for this disease, they need sufficient professional annotation data to train the model, requiring more expensive and time-consuming screening skills.

Method

This study aims to economize manual power and proposes a deep graph correlation network (DGCN) to develop automated diabetic retinopathy grading without any professional annotations. DGCN involves the novel deep learning algorithm of a graph convolutional network to exploit inherent correlations from independent retinal image features learned by a convolutional neural network. Three designed loss functions of graph-center, pseudo-contrastive, and transformation-invariant constrain the optimisation and application of the DGCN model in an automated diabetic retinopathy grading task.

Results

To evaluate the DGCN model, this study employed EyePACS-1 and Messidor-2 sets to perform grading results. It achieved an accuracy of 89.9% (91.8%), sensitivity of 88.2% (90.2%), and specificity of 91.3% (93.0%) on EyePACS-1 (Messidor-2) data set with a confidence index of 95% and commendable effectiveness on receiver operating characteristic (ROC) curve and t-SNE plots.

Conclusion

The grading capability of this study is close to that of retina specialists, but superior to that of trained graders, which demonstrates that the proposed DGCN provides an innovative route for automated diabetic retinopathy grading and other computer-aided diagnostic systems.
Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough
Authors: Chen Ye , Xu Cuiling , Wu Nengyou , Sun Zhilei , Liu Changling , Zhen Yu , Xin Youzhi , Zhang Xilin , Geng Wei , Cao Hong , Zhai Bin , Li Jing , Qin Shuangshuang , Zhou Yucheng
Published: April 2022

Active cold seeps in the Okinawa Trough (OT) have been widely identified, but the sediment microbial communities associated with these sites are still poorly understood. Here, we investigated the distribution and biomass of the microbial communities, particularly those associated with the anaerobic oxidation of methane (AOM), in sediments from an active cold seep in the mid-Okinawa Trough. Methane-oxidizing archaea, including ANME-1a, ANME-1b, ANME-2a/b, ANME-2c, and ANME-3, were detected in the OT cold seep sediments. Vertical stratification of anaerobic methanotrophic archaea (ANME) communities was observed in the following order: ANME-3, ANME-1a, and ANME-1b. In addition, the abundance of methyl coenzyme M reductase A (mcrA) genes corresponded to high levels of dissolved iron, suggesting that methane-metabolizing archaea might participate in iron reduction coupled to methane oxidation (Fe-AOM) in the OT cold seep. Furthermore, the relative abundance of ANME-1a was strongly related to the concentration of dissolved iron, indicating that ANME-1a is a key microbial player for Fe-AOM in the OT cold seep sediments. Co-occurrence analysis revealed that methane-metabolizing microbial communities were mainly associated with heterotrophic microorganisms, such as JS1, Bathy-1, and Bathy-15.
Treatment of Axillary Osmidrosis by Rebalancing Skin Microecology With Lactobacillus bulgaricus
Authors: Li Pinglu , Chen Shuyue , Li Ping , Xu Dan , Tang Xueyuan , Liao Junlin , Xie Hongju , Li Gaofeng , Kuang Yehong , Su Juan , Tang Shijie , Zhou Jianda
Published: April 2022

Corynebacterium accounts for around 20% of the armpit microbiome and plays an essential role in axillary osmidrosis (AO). In this study, the effects of Lactobacillus bulgaricus treatment on the microecological environment of armpits and its efficacy in the treatment of AO were investigated. A total of 10 AO patients were enrolled in this study. The patients were treated with L. bulgaricus mixed with saline on the left armpit (experimental group) and pure saline on the right armpit (control group) for 28 days. After treatment, AO severity showed a significant decrease (p = 0.013) in the experimental group compared with the control group, and the Corynebacterium abundance also showed a corresponding significant decrease (p < 0.01). Moreover, no significant variation in Staphylococcus abundance was found between these two groups. The microbe diversity is not disturbed in the treatment. Accordingly, our study demonstrates that L. bulgaricus can serve as an effective probiotic microbe for AO treatment by reducing the abundance of Corynebacterium and rebalancing the microecological environment.
Nuclear and Mitochondrial SSU rRNA Genes Reveal Hidden Diversity of Haptophrya Endosymbionts in Freshwater Planarians and Challenge Their Traditional Classification in Astomatia
Authors: Rataj Matej , Zhang Tengyue , Vd’ačný Peter
Published: April 2022

Like many other aquatic animals, freshwater planarians have also become partners of symbiotic ciliates from the class Oligohymenophorea. In the present study, we explored the hidden diversity and addressed the questionable systematic position of mouthless obligatory gut endosymbionts of freshwater planarians, using the nuclear and mitochondrial SSU rRNA genes. Although all isolated ciliates morphologically corresponded to a single species, molecular analyses suggested the existence of three genetically distinct entities: Haptophrya planariarum, Haptophrya dugesiarum nov. spec., and Haptophrya schmidtearum nov. spec. The two former species share the same planarian host, which indicates a speciation model involving one duplication event without host switching. Such a diversification pattern was recognized also in astome ciliates inhabiting megascolecid and glossoscolecid earthworms. The present multi-gene phylogenies along with the secondary structure of the mitochondrial 16S rRNA molecule, however, challenge the traditional classification of Haptophrya within the subclass Astomatia. Haptophrya very likely evolved from an orphan scuticociliate lineage by the loss of oral apparatus and by the transformation of the thigmotactic field into an adhesive sucker. Since astomy evolved multiple times independently within the Oligohymenophorea, the loss of cell mouth cannot be used as a sole argument for the assignment of Haptophrya to the Astomatia anymore.