bluuebunny/crossref_metadata_2025 huggingface dataset:
Protocol for establishing a model for integrated influenza surveillance in Tamil Nadu, India
Authors: Abdulkader Rizwan S. , Potdar Varsha , Mohd Gulam , Chadwick Joshua , Raju Mohan Kumar , Devika S. , Bharadwaj Sumit Dutt , Aggarwal Neeraj , Vijay Neetu , Sugumari C. , Sundararajan T. , Vasuki V. , Bharathi Santhose N. , Mohammed Razik C. A. , Madhavan Vinoth , Krupa N. C. , Prabakaran Nandhini , Murhekar Manoj V. , Gupta Nivedita
Published: August 2023

The potential for influenza viruses to cause public health emergencies is great. The World Health Organisation (WHO) in 2005 concluded that the world was unprepared to respond to an influenza pandemic. Available surveillance guidelines for pandemic influenza lack the specificity that would enable many countries to establish operational surveillance plans. A well-designed epidemiological and virological surveillance is required to strengthen a country’s capacity for seasonal, novel, and pandemic influenza detection and prevention. Here, we describe the protocol to establish a novel mechanism for influenza and SARS-CoV-2 surveillance in the four identified districts of Tamil Nadu, India. This project will be carried out as an implementation research. Each district will identify one medical college and two primary health centres (PHCs) as sentinel sites for collecting severe acute respiratory infections (SARI) and influenza like illness (ILI) related information, respectively. For virological testing, 15 ILI and 10 SARI cases will be sampled and tested for influenza A, influenza B, and SARS-CoV-2 every week. Situation analysis using the WHO situation analysis tool will be done to identify the gaps and needs in the existing surveillance systems. Training for staff involved in disease surveillance will be given periodically. To enhance the reporting of ILI/SARI for sentinel surveillance, trained project staff will collect information from all ILI/SARI patients attending the sentinel sites using pre-tested tools. Using time, place, and person analysis, alerts for abnormal increases in cases will be generated and communicated to health authorities to initiate response activities. Advanced epidemiological analysis will be used to model influenza trends over time. Integrating virological and epidemiological surveillance data with advanced analysis and timely communication can enhance local preparedness for public health emergencies. Good quality surveillance data will facilitate an understanding outbreak severity and disease seasonality. Real-time data will help provide early warning signals for prevention and control of influenza and COVID-19 outbreaks. The implementation strategies found to be effective in this project can be scaled up to other parts of the country for replication and integration.
Mapping cover crop species in southeastern Michigan using Sentinel-2 satellite data and Google Earth Engine
Authors: Wang Xuewei , Blesh Jennifer , Rao Preeti , Paliwal Ambica , Umashaanker Maanya , Jain Meha
Published: August 2023

Cover crops are a critical agricultural practice that can improve soil quality, enhance crop yields, and reduce nitrogen and phosphorus losses from farms. Yet there is limited understanding of the extent to which cover crops have been adopted across large spatial and temporal scales. Remote sensing offers a low-cost way to monitor cover crop adoption at the field scale and at large spatio-temporal scales. To date, most studies using satellite data have mapped the presence of cover crops, but have not identified specific cover crop species, which is important because cover crops of different plant functional types (e.g., legumes, grasses) perform different ecosystem functions. Here we use Sentinel-2 satellite data and a random forest classifier to map the cover crop species cereal rye and red clover, which represent grass and legume functional types, in the River Raisin watershed in southeastern Michigan. Our maps of agricultural landcover across this region, including the two cover crop species, had moderate to high accuracies, with an overall accuracy of 83%. Red clover and cereal rye achieved F1 scores that ranged from 0.7 to 0.77, and user's and producer's accuracies that ranged from 63.3% to 86.2%. The most common misclassification of cover crops was fallow fields with remaining crop stubble, which often looked similar because these cover crop species are typically planted within existing crop stubble, or interseeded into a grain crop. We found that red-edge bands and images from the end of April and early July were the most important for classification accuracy. Our results demonstrate the potential to map individual cover crop species using Sentinel-2 imagery, which is critical for understanding the environmental outcomes of increasing crop diversity on farms.
Oral inflammatory load predicts vascular function in a young adult population: a pilot study
Authors: Hong Ker-Yung , Ghafari Avin , Mei Yixue , Williams Jennifer S. , Attia Dina , Forsyth Jourdyn , Wang Kevin , Wyeld Trevor , Sun Chunxiang , Glogauer Michael , King Trevor J.
Published: August 2023

Background

The periodontium is a highly vascularized area of the mouth, and periodontitis initiates negative functional and structural changes in the vasculature. However, mild oral inflammation, including levels experienced by many apparently healthy individuals, has an unclear impact on cardiovascular function. The purpose of this pilot study is to investigate the effects of objectively measured whole mouth oral inflammatory load (OIL) on vascular function in apparently healthy individuals.

Methods

In this cross-sectional and correlational analysis, we recruited 28 young (18–30 years) and systemically healthy participants (16 male, 12 female). Using oral neutrophil counts, a validated measure for OIL, we collected participant's mouth rinse samples and quantified OIL. Blood pressure, arterial stiffness (pulse-wave velocity) and endothelial function (brachial artery flow-mediated dilation) were also measured.

Results

Only oral neutrophil count significantly predicted flow-mediated dilation % (p = 0.04; R2 = 0.16, β = − 1.05) and those with OIL levels associated with >2.5 × 105 neutrophil counts (n = 8) had a lower flow-mediated dilation % (6.0 ± 2.3%) than those with counts associated with gingival health with less than 2.5 × 105 neutrophil counts (10.0 ± 5.2%, p = 0.05). There were no significant predictors for arterial stiffness.

Conclusion

We found that OIL was a predictor of reduced flow-mediated dilation. An impairment in flow-mediated dilation is an indicator of future possible risk of cardiovascular disease—one of the leading causes of death in North America. Therefore, this study provides evidence for the importance of oral health and that OIL may impact endothelial function.
Phase-only array transmit beamforming without iterative/numerical optimization methods
Authors: Orlando Danilo , Farina Alfonso
Published: August 2023

In this letter, we address the problem of phase-only transmit beamforming to generate a wide beam with an almost flat mainlobe for phased arrays. Instead of resorting to time-demanding optimization procedures, the proposed method is grounded on the Fourier analysis and exploits the fact that radiation pattern can be written as the Fourier transform of the aperture illumination function. In this context, we consider a complex linear frequency modulated illumination function and derive the equations allowing for a control of the beam width. The related computational complexity is linear in the number of the array elements. The numerical examples show the effectiveness of the proposed method in forcing the desired beam shape with good sidelobes’ properties and also in comparison with an iterative competitor.
Bedtime routines, development, and caregiver educational attainment in toddlerhood
Authors: Lam Joey Tsz Ying , Williamson Ariel A. , Salih Zainab , Heere Megan , Mindell Jodi A.
Published: August 2023

Background

Implementing a consistent bedtime routine is an empirically supported intervention to improve sleep in toddlers, but little is known about its association with social-emotional outcomes, and among children living in lower socioeconomic status (SES) contexts.

Objective

This study examined the longitudinal associations between bedtime routines, social-emotional development, and caregiver educational attainment in toddlers presenting to primary care.

Method

Caregivers of 40 toddlers (Mage = 12.85 months, 57.5% female, 62.5% Black/African American) completed questionnaires on sociodemographic factors and child bedtime routine consistency at their 12-month well visit. At the 15- and 24-month well visits, data were collected on child bedtime routine consistency and social-emotional development, including the Brief Infant-Toddler Social and Emotional Assessment (BITSEA) for social-emotional problems and competency and the Ages and Stages Questionnaire (3rd edition; ASQ-3) to assess communication and personal-social skills.

Results

Overall, the majority of families engaged in a consistent bedtime routine (≥5 nights/week) at all time points (63% at 12 months, 75% at 15 months, and 86% at 24 months). Controlling for concurrent bedtime routine consistency, toddlers with a more consistent bedtime routine at 12 months exhibited less dysregulation at age 15 months. Toddlers without a consistent bedtime routine at 15 months exhibited more externalizing and internalizing problems and dysregulation at 24 months. Furthermore, there was a significant interaction between bedtime routine consistency at 15 months and caregiver education for internalizing problems at 24 months, such that toddlers of caregivers with a high school education or less who lacked a consistent bedtime routine showed the most internalizing problems.

Conclusion

Clinicians should consider recommending that families with toddlers incorporate a nightly bedtime routine not only to improve overall sleep health, but also to potentially optimize toddlers' positive social-emotional and behavioral trajectories, especially in families with lower educational attainment.
In vitro ruminal fermentation and cow-to-mouse fecal transplantations verify the inter-relationship of microbiome and metabolome biomarkers: potential to promote health in dairy cows
Authors: Hsieh Jui-Chun , Chuang Shih-Te , Hsu Yu-Ting , Ho Shang-Tse , Li Kuan-Yi , Chou Shih-Hsuan , Chen Ming-Ju
Published: August 2023

Introduction

There are differences in the gut microbiome and metabolome when the host undergoes different physical or pathological conditions. However, the inter-relationship of microbiome and metabolome biomarkers to potentially promote the health of dairy cows needs to be studied. Further, the development of next-generation probiotics for dairy cattle health promotion has not been demonstrated.

Objective

In the present study, we identified the microbiome and metabolome biomarkers associated with healthy cows.

Methods

We analyzed the relationships of the ruminal microorganism profile and metabolites between healthy and mastitis lactating dairy cows. The roles of bacterial biomarker were further verified by in vitro fermentation and cow-to-mouse fecal microbiota transplantation (FMT).

Results

Two species, Ruminococcus flavefaciens and Bifidobacterium longum subsp. longum, and six rumen metabolites were positively correlated with healthy cows by Spearman’s correlation analysis. Through in vitro ruminal fermentation, inoculating R. flavefaciens and B. longum subsp. longum showed the upregulation of the levels of putrescine, xanthurenic acid, and pyridoxal in the mastitis ruminal fluid, which confirmed the inter-relationships between these microbiota and metabolites associated with healthy cows. Further, we verified the role of R. flavefaciens and B. longum subsp. longum in promoting health by FMT. The administration of R. flavefaciens and B. longum subsp. longum reduced the death rate and recovered the bodyweight loss of germ-free mice caused by FMT mastitis feces.

Discussion

We provided evidence that the bacterial biomarkers alter downstream metabolites. This could indirectly indicate that the two bacterial biomarkers have the potential to be used as next-generation probiotics for dairy cattle, although it needs more evidence to support our hypothesis. Two species, R. flavefaciens and B. longum subsp. longum, with three metabolites, putrescine, xanthurenic acid, and pyridoxal, identified in the ruminal fluid, may point to a new health-promoting and disease-preventing approach for dairy cattle.
A survey of biosecurity practices of pig farmers in selected districts affected by African swine fever in Uganda
Authors: Ekakoro John E. , Nawatti Margaret , Singler David F. , Ochoa Krista , Kizza Robinah , Ndoboli Dickson , Ndumu Deo B. , Wampande Eddie M. , Havas Karyn A.
Published: August 2023

Introduction

In Uganda, pig production is an important source of livelihood for many people and contributes to food security. African swine fever (ASF) is a major constraint to pig production in Uganda, threatening the food supply and sustainable livelihoods. Prevention of ASF primarily relies on good biosecurity practices along the pig value chain. Previous studies showed that biosecurity along the pig value chain and on farms in Uganda is poor. However, the biosecurity practices of pig farmers in ASF affected areas of Uganda and their opinions on on-farm ASF morbidity and mortality were previously not comprehensively characterized. The objectives of this study were to document pig farmers’ experiences with ASF in their farms and to describe the pig biosecurity practices in districts of Uganda that were highly affected by ASF.

Methods

A total of 99 farmers were interviewed in five districts. Data were collected by way of triangulation through farmer interviews, field observations during the farmer interviews, and a survey of key informants. However, farmer interviews were considered the primary source of data for this study. Farmers’ biosecurity practices were scored using a biosecurity scoring algorithm.

Results

Forty-one out of 96 (42.7%) farmers reported having pigs with ASF in the past 12 months. The level of pig farming experience (p = 0.0083) and herd size (p < 0.0001) were significantly associated with the reported occurrence of ASF. Overall, the biosecurity scores for the respondents were considered poor with 99% (98/99) scoring <70% and just one farmer obtaining a fair score of 72.2%. District (p = 0.0481), type of husbandry system (p = 0.014), and type of pig breed raised (p = 0.004) were significantly associated with farmer’s biosecurity score.

Conclusion

Continued farmer education on ASF and the importance of good biosecurity practices is necessary. More in-depth scientific inquiry into the factors influencing the biosecurity practices among pig farmers in Uganda is necessary.
Intramolecular interaction of NEP regulated by CRM1 ensures the unidirectional transport of M1 for the nuclear export of influenza viral ribonucleoprotein
Authors: Hirohama Mikako , Yamashita Shun , Asaka Masamitsu N. , Kuroki Takahiro , Kawaguchi Atsushi
Published: August 2023

Introduction

The influenza virus genome consists of single-stranded RNAs and forms viral ribonucleoprotein (RNP) complexes. After viral genome replication in the nucleus, the viral RNP interacts with viral protein M1. The M1-viral RNP complex is exported to the cytoplasm via the CRM1-dependent pathway using NS2/NEP as an export adaptor protein. NEP is a 14 kDa protein and diffusely localizes in the nucleus and cytoplasm. Upon binding to the NLS motif of M1, NEP inhibits the nuclear accumulation of M1 and promotes the nuclear export of M1-viral RNP complex. However, the detail mechanism by which NEP binds to M1 only in the nucleus remains unclear.

Methods

To visualize the interaction of NEP with M1 in the formation of vRNP export complexes, we performed in situ proximity ligation assays. The close proximity of N-terminal and C-terminal domains of NEP was tested by split Renilla luciferase complementation assays in which the N-terminal and C-terminal fragments of Renilla luciferase were fused to the N-terminus and C-terminus of NEP, respectively.

Results and discussion

We found that the intramolecular interaction of NEP inhibits the interaction of NEP with M1. The intramolecular interaction of NEP was mediated through the interaction of the N-terminal NES motif with the M1-binding domain at the C-terminus. By adding leptomycin B, a potent inhibitor of CRM1, the interaction of NEP with M1 was impaired. These results suggest that CRM1 disrupts the intramolecular interaction of NEP by recognizing the NES motif at the N-terminus of NEP, thereby promoting the interaction of NEP with M1. We also found that NEP mutant deficient in the intramolecular interaction was co-localized with M1 at the plasma membrane and did not show nuclear localization with M1. Based on these results, we propose that the intramolecular interaction of NEP regulated by CRM1 ensures the unidirectional transport of M1.
A Three-Dimensional Body Force Modeling of Fans in Windmilling Condition and Its Application
Authors: Kong Qingguo , Jia Wei
Published: August 2023

To investigate the aerodynamic characteristics of the fan in windmilling conditions, a new body force model with the fan rotational speed prediction model was developed. The fan rotational speed prediction model was built based on the balance of fan output torque and resistance torque. The rotational speed of the fan spool can be iteratively solved simultaneously with solving the governing equations without requiring mass flow rate or other inputs. The comparison with the experimental results shows that using the body force model can accurately predict the rotational speed of the fan spool under different operating conditions. The radial distribution of flow parameters can be obtained. Moreover, numerical simulations of the fan under different circumferential total pressure distortion inflow conditions were conducted using the body force model. The results show that, unlike the design point and non-design point at which the fan operates normally, the high radius region of the fan is in the “turbine mode” while the low radius region is in the “compressor mode” under windmilling conditions. The different effects on the longitudinal vortex in the two regions deepen and alleviate the circumferential distortion, respectively. There are strong circumferential and radial pressure gradients at the junction of the distortion-affected zone and the non-distortion-affected zone, adding additional mixing losses.
Discrimination of Anari Cheese Samples in Comparison with Halloumi Cheese Samples Regarding the Origin of the Species by FTIR Measurements and Chemometrics
Authors: Tarapoulouzi Maria , Theocharis Charis R.
Published: August 2023

Nowadays, adulteration of traditional food products is a very important field in the general food authenticity sector. Moreover, it is important to create databases with authentic traditional products. In Cyprus, research about the traditional dairy products is scarce. Anari is predominantly made from goat’s and sheep’s milk, but milk from cows can also be used. It is produced during the process of Halloumi cheese making. Classification of Halloumi and Anari cheese took place in two classes, thus per cheese type, and after that in milk species subclasses such as cow and goat-sheep origins for each cheese type. This research study aims to enlighten the field of food authenticity in terms of traditional Cypriot dairy products. The first step of the methodology is the freeze-drying process for lyophilization of samples. Forty-four samples have been analyzed thus far, including both Halloumi and Anari cheese. Measurements for each sample were obtained by using Fourier Transformed Infrared (FTIR) Spectroscopy. Interpretation of the extensive data was undertaken via Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA), and through the SIMCA chemometrics package. Characterization of Anari cheese by applying FTIR took place. In terms of cheese type, Halloumi and Anari cheese samples were classified correctly. In addition, Anari samples were classified correctly regarding the milk species’ origin. The proposed experimental procedure along with chemometrics allow the detection of the milk species’ origin of traditional Anari samples, highlighting the importance of FTIR spectroscopy in combination with chemometrics in food authenticity. Creation of a database with Anari samples from Cyprus has started, and this is a very important step towards authenticity of traditional dairy products of Cyprus.
The Response to Dietary Leucine in Laying Lens
Authors: Sakomura Nilva Kazue , Reis Matheus , Bonagurio Lucas Pimentel , Leme Bruno Balbino , Viana Gabriel Silva , Melaré Mirella Cunha , Gous Robert Mervyn
Published: August 2023

This study aimed to estimate the standardized ileal digestible leucine intake (SID-Leui, mg) in laying hens for maintenance, and to describe the response in laying hens to SID-Leui, thereby providing the information required to determine the optimum economic intake of SID-Leu for laying hens. Two nitrogen balance series, one balanced and the other unbalanced with respect to leucine (Leu), were used to estimate the SID-Leu requirement for maintenance using 36 roosters per series. The roosters were randomly distributed among the six levels of Leu with each level being replicated six times. The six diets were formulated to contain 0.0, 3.5, 6.9, 10.4, 13.9, or 17.4 g/kg of SID-Leu for the unbalanced series and 0.0, 4.0, 8.0, 12.0, 16.0, or 20.0 g/kg of SID-Leu for the balanced series. The SID-Leui maintenance requirement was calculated as 144 mg/bird d, 66 mg/kg d, 74 mg/kg0.75d or 395 mg/BPm0.73d. For the response trial, 120 individually caged laying hens (63 weeks old) were randomly distributed among eight treatments with 15 replicates. To estimate the SID-Leui for the population of hens, the Reading Model was fitted to the data using body weight (BW, kg), SID-Leui and egg output (EO, g). The Reading Model calculated the mg SID-Leui = 11.6 EOmax + 43.4 BW. The efficiency of SID-Leu utilization for laying hens was estimated to be 79%.
The Thoracic Inlet Length as a Reference Point to Radiographically Assess Cardiac Enlargement in Dogs with Myxomatous Mitral Valve Disease
Authors: Marbella Fernández David , García Verónica , Santana Alexis José , Montoya-Alonso José Alberto
Published: August 2023

The diagnostic value of the vertebral heart size (VHS) in dogs with mitral valve degeneration (MVD) is compromised when middle thoracic vertebral anomalies are present. The objective of this study was to assess the use of the thoracic inlet heart score (TIHS) to identify left heart enlargement (LHE) secondary to MVD. The cardiac silhouette of 50 clinically healthy dogs and 106 MVD dogs in different stages was assessed on a right lateral chest radiograph. The TIHS and VHS value were calculated for each patient and compared. The TIHS was significantly different between the control dogs and the dogs with MMVD, increasing with disease stage, control 2.91 ± 0.23, Stage B1 2.98 ± 0.36, B2 3.25 ± 0.34, and C 3.53 ± 0.36, p < 0.05. A THIS ≥3.3 showed 69% sensitivity and 81% specificity to identify LHE. The TIHS showed moderate correlation with the VHS, LA/Ao, and LVIDDN 0.59, 0.42, and 0.62, respectively. The intraobserver and interobserver agreement were almost perfect, 0.96, and substantial, 0.73. The TIHS method can be used to identify LHE secondary to MMVD on dogs’ thoracic radiographs.
Copper(II) Complexes with Carnosine Conjugates of Hyaluronic Acids at Different Dipeptide Loading Percentages Behave as Multiple SOD Mimics and Stimulate Nrf2 Translocation and Antioxidant Response in In Vitro Inflammatory Model
Authors: Bellia Francesco , Lanza Valeria , Naletova Irina , Tomasello Barbara , Ciaffaglione Valeria , Greco Valentina , Sciuto Sebastiano , Amico Pietro , Inturri Rosanna , Vaccaro Susanna , Campagna Tiziana , Attanasio Francesco , Tabbì Giovanni , Rizzarelli Enrico
Published: August 2023

A series of copper(II) complexes with the formula [Cu2+Hy(x)Car%] varying the molecular weight (MW) of Hyaluronic acid (Hy, x = 200 or 700 kDa) conjugated with carnosine (Car) present at different loading were synthesized and characterized via different spectroscopic techniques. The metal complexes behaved as Cu, Zn-superoxide dismutase (SOD1) mimics and showed some of the most efficient reaction rate values produced using a synthetic and water-soluble copper(II)-based SOD mimic reported to date. The increase in the percentage of Car moieties parallels the enhancement of the I50 value determined via the indirect method of Fridovich. The presence of the non-functionalized Hy OH groups favors the scavenger activity of the copper(II) complexes with HyCar, recalling similar behavior previously found for the copper(II) complexes with Car conjugated using β-cyclodextrin or trehalose. In keeping with the new abilities of SOD1 to activate protective agents against oxidative stress in rheumatoid arthritis and osteoarthritis diseases, Cu2+ interaction with HyCar promotes the nuclear translocation of erythroid 2-related factor that regulates the expressions of target genes, including Heme-Oxigenase-1, thus stimulating an antioxidant response in osteoblasts subjected to an inflammatory/oxidative insult.
Fibroblast Upregulation of Vitamin D Receptor Represents a Self-Protective Response to Limit Fibroblast Proliferation and Activation during Pulmonary Fibrosis
Authors: Wei Juan , Zhan Junhui , Ji Hui , Xu Yitong , Xu Qingfeng , Zhu Xiaoyan , Liu Yujian
Published: August 2023

Dysregulation of vitamin D receptor (VDR) is implicated in chronic obstructive pulmonary disease. However, whether VDR dysregulation contributes to the development of pulmonary fibrosis remains largely unknown. Analysis of bulk and single-cell RNA profiling datasets revealed VDR upregulation in lung fibroblasts from patients with pulmonary fibrosis or fibrotic mice, which was validated in lung fibroblasts from bleomycin-exposed mice and bleomycin-treated fibroblasts. Stable VDR knockdown promoted, whereas the VDR agonist paricalcitol suppressed lung fibroblast proliferation and activation. Gene set enrichment analysis (GSEA) showed that the JAK/STAT pathway and unfolded protein response (UPR), a process related to endoplasmic reticulum (ER) stress, were enriched in lung fibroblasts of fibrotic lungs. Stable VDR knockdown stimulated, but paricalcitol suppressed ER stress and JAK1/STAT3 activation in lung fibroblasts. The STAT3 inhibitor blocked bleomycin- or stable VDR knockdown-induced ER stress. Paricalcitol inhibited the bleomycin-induced enrichment of STAT3 to the ATF6 promoter, thereby suppressing ATF6 expression in fibroblasts. Paricalcitol or intrapulmonary VDR overexpression inactivated JAK1/STAT3 and suppressed ER stress in bleomycin-treated mice, thus resulting in the inhibition of fibroblast proliferation and activation. Collectively, this study suggests that fibroblast VDR upregulation may be a self-protective response to limit fibroblast proliferation and activation during pulmonary fibrosis by suppressing the JAK1/STAT3/ER stress pathway.
A Nonlinear Convex Decreasing Weights Golden Eagle Optimizer Technique Based on a Global Optimization Strategy
Authors: Deng Jiaxin , Zhang Damin , Li Lun , He Qing
Published: August 2023

A novel approach called the nonlinear convex decreasing weights golden eagle optimization technique based on a global optimization strategy is proposed to overcome the limitations of the original golden eagle algorithm, which include slow convergence and low search accuracy. To enhance the diversity of the golden eagle, the algorithm is initialized with the Arnold chaotic map. Furthermore, nonlinear convex weight reduction is incorporated into the position update formula of the golden eagle, improving the algorithm’s ability to perform both local and global searches. Additionally, a final global optimization strategy is introduced, allowing the golden eagle to position itself in the best possible location. The effectiveness of the enhanced algorithm is evaluated through simulations using 12 benchmark test functions, demonstrating improved optimization performance. The algorithm is also tested using the CEC2021 test set to assess its performance against other algorithms. Several statistical tests are conducted to compare the efficacy of each method, with the enhanced algorithm consistently outperforming the others. To further validate the algorithm, it is applied to the cognitive radio spectrum allocation problem after discretization, and the results are compared to those obtained using traditional methods. The results indicate the successful operation of the updated algorithm. The effectiveness of the algorithm is further evaluated through five engineering design tasks, which provide additional evidence of its efficacy.
Modeling of a Rotary Adsorber for Continuous Capture of Indoor Carbon Dioxide
Authors: Liu Lumeng , Wan Ning , Zeng Wenmao , Shi Jiachen , Liu Meng , Liu Huan
Published: August 2023

Removing indoor CO2 as a pollutant via solid sorbents is a promising solution to maintaining acceptable indoor air quality while minimizing the energy consumption of ventilation. Compared to fixed-bed and fluidized-bed configurations, which require at least two beds to allow for continuous operation, a rotary adsorber is more compact and suitable to be integrated into the ventilation systems of buildings. In the present study, a regenerative rotary adsorber based on temperature swing adsorption was modeled to investigate continuous CO2 capture in an indoor environment. The governing equations of heat and mass transfer processes associated with the capture were established and coded in ANSYS Fluent software. The spatiotemporal variations of CO2 concentration and temperature in gas and solid phases within the rotary adsorber were obtained. The key findings are: (1) adjusting the speed mainly affects circumferential concentration and temperature distribution, but has little impact on axial concentration and temperature; (2) Increasing desorption inlet flow rate has little impact on adsorption outlet concentration, but significantly decreases desorption outlet concentration; (3) Raising desorption inlet temperature can increase both adsorption and desorption outlet average concentrations; (4) Reducing the volume proportion of the desorption sector will slightly increase adsorption outlet concentration and slightly decrease desorption outlet concentration, but barely affects average adsorption and desorption outlet temperatures.
Fast and Non-Invasive Evaluation of Yeast Viability in Fermentation Processes Using Raman Spectroscopy and Machine Learning
Authors: Heese Raoul , Wetschky Jens , Rohmer Carina , Bailer Susanne M. , Bortz Michael
Published: August 2023

Fermentation processes used for producing alcoholic beverages such as beer, wine, and cider have a long history, having been developed early on across different civilizations. In most instances, yeast strains are used for fermentation processes, e.g., at breweries and wineries. Monitoring of yeast viability, cell count, and growth behavior is essential to ensure a controlled fermentation process. However, classical microbiological techniques to monitor fermentation process parameters are time-consuming and require sampling, along with the risk of contamination. Nowadays, industries are moving toward automation and digitalization. This necessitates state-of-the-art process analytical technologies to ensure an efficient and controlled process to obtain high-quality product outputs. Hence, there is a strong need for a fast, non-invasive, and generally applicable method to evaluate the viability of yeast cells during fermentation to warrant the standardization and purity of produced products in industrial applications. The aim of our study is to discriminate between viable and non-viable yeast in various culture media using Raman spectroscopy (RS) followed by data analysis with machine learning (ML) tools. These techniques allow for rapid, non-invasive analysis addressing the limitations of traditional methods. The present work primarily focuses on the evaluation of RS combined with predictive ML models in a non-real-time setting. Our goal is to adapt these techniques for future application in real-time monitoring and determination of yeast viability in biotechnological processes. We demonstrate that RS, in combination with ML, is a promising tool for non-invasive inline monitoring of fermentation processes.
Preeclampsia and the Antiphospholipid Syndrome
Authors: Mayer-Pickel Karoline , Nanda Manurishi , Gajic Maja , Cervar-Zivkovic Mila
Published: August 2023

Antiphospholipid syndrome (APS) is characterized by venous or arterial thrombosis and/or adverse pregnancy outcome in the presence of persistent laboratory evidence of antiphospholipid antibodies (aPLs). Preeclampsia complicates about 10–17% of pregnancies with APS. However, only early onset preeclampsia (<34 weeks of gestation) belongs to the clinical criteria of APS. The similarities in the pathophysiology of early onset preeclampsia and APS emphasize an association of these two syndromes. Overall, both are the result of a defective trophoblast invasion and decidual transformation at early gestation. Women with APS are at increased risk for prematurity; the reasons are mostly iatrogenic due to placental dysfunction, such as preeclampsia or FGR. Interestingly, women with APS have also an increased risk for preterm delivery, even in the absence of FGR and preeclampsia, and therefore it is not indicated but spontaneous. The basic treatment of APS in pregnancy is low-dose aspirin and low-molecular-weight heparin. Nevertheless, up to 20–30% of women develop complications at early and late gestation, despite basic treatment. Several additional treatment options have been proposed, with hydroxychloroquine (HCQ) being one of the most efficient. Additionally, nutritional interventions, such as intake of vitamin D, have shown promising beneficial effects. Curcumin, due to its antioxidant and anti-inflammatory properties, might be considered as an additional intervention as well.
A Multicenter Retrospective Chart Review Study of Treatment and Disease Patterns and Clinical Outcomes of Patients with Chronic-Phase Chronic Myeloid Leukemia in Third-Line Treatment or with T315I Mutation
Authors: Nicolini Franck-Emmanuel , Huguet Françoise , Huynh Lynn , Xu Churong , Bouvier Christophe , Yocolly Aurore , Etienne Gabriel
Published: August 2023

This retrospective chart review study investigated the clinical burden of adult patients with chronic-phase chronic myeloid leukemia (CP-CML) treated at three centers in France (2006–2021) who failed on two or more tyrosine kinase inhibitors (TKIs; third-line [3L]+ cohort) or harbored the BCR::ABL1 T315I mutation (T315I cohort). In the 3L+ cohort (N = 157; median age at diagnosis, 56 years), TKIs received in 3L (median duration: 17 months) were dasatinib (32%), nilotinib (19%), imatinib (18%), ponatinib (17%), and bosutinib (14%). Of the 145 patients with documented responses in 3L, 42% experienced major molecular response (MMR) at 12 months. Median event-free survival [95% confidence interval] was 53.6 [44.0, 67.5] months, and median progression-free survival and overall survival (OS) were not reached. Achieving MMR in 3L was associated with a decreased mortality risk. In the T315I cohort (N = 17; 52 years), 41% of patients received five or more lines of therapy. Following identification of the T315I mutation, ponatinib was the most common TKI used (59%); the median [interquartile range] OS was 5 [3–10] years. The most common adverse events were infections (3L+ cohort) and thrombocytopenia (T315I cohort) (both 18%). Well-tolerated therapies that achieve durable responses are needed in 3L or earlier to improve CP-CML prognosis.
Identifying Cancer Type-Specific Transcriptional Programs through Network Analysis
Authors: Kurup Jiji T. , Kim Seongho , Kidder Benjamin L.
Published: August 2023

Identifying cancer type-specific genes that define cell states is important to develop effective therapies for patients and methods for detection, early diagnosis, and prevention. While molecular mechanisms that drive malignancy have been identified for various cancers, the identification of cell-type defining transcription factors (TFs) that distinguish normal cells from cancer cells has not been fully elucidated. Here, we utilized a network biology framework, which assesses the fidelity of cell fate conversions, to identify cancer type-specific gene regulatory networks (GRN) for 17 types of cancer. Through an integrative analysis of a compendium of expression data, we elucidated core TFs and GRNs for multiple cancer types. Moreover, by comparing normal tissues and cells to cancer type-specific GRNs, we found that the expression of key network-influencing TFs can be utilized as a survival prognostic indicator for a diverse cohort of cancer patients. These findings offer a valuable resource for exploring cancer type-specific networks across a broad range of cancer types.
Improved Characterization of Circulating Tumor Cells and Cancer-Associated Fibroblasts in One-Tube Assay in Breast Cancer Patients Using Imaging Flow Cytometry
Authors: Muchlińska Anna , Wenta Robert , Ścińska Wiktoria , Markiewicz Aleksandra , Suchodolska Grażyna , Senkus Elżbieta , Żaczek Anna J. , Bednarz-Knoll Natalia
Published: August 2023

Circulating tumor cells (CTCs) and circulating cancer-associated fibroblasts (cCAFs) have been individually considered strong indicators of cancer progression. However, technical limitations have prevented their simultaneous analysis in the context of CTC phenotypes different from epithelial. This study aimed to analyze CTCs and cCAFs simultaneously in the peripheral blood of 210 breast cancer patients using DAPI/pan-keratin (K)/vimentin (V)/alpha-SMA/CD29/CD45/CD31 immunofluorescent staining and novel technology—imaging flow cytometry (imFC). Single and clustered CTCs of different sizes and phenotypes (i.e., epithelial phenotype K+/V− and epithelial–mesenchymal transition (EMT)-related CTCs, such as K+/V+, K−/V+, and K−/V−) were detected in 27.6% of the samples and correlated with metastases. EMT-related CTCs interacted more frequently with normal cells and tended to occur in patients with tumors progressing during therapy, while cCAFs coincided with CTCs (mainly K+/V− and K−/V−) in seven (3.3%) patients and seemed to correlate with the presence of metastases, particularly visceral ones. This study emphasizes the advantages of imFC in the field of liquid biopsy and highlights the importance of multimarker-based analysis of different subpopulations and phenotypes of cancer progression-related cells, i.e., CTCs and cCAFs. The co-detection of CTCs and cCAFs might improve the identification of patients at higher risk of progression and their monitoring during therapy.
Standard-Cell-Based Comparators for Ultra-Low Voltage Applications: Analysis and Comparisons
Authors: Della Sala Riccardo , Centurelli Francesco , Scotti Giuseppe , Palumbo Gaetano
Published: August 2023

This work is focused on the performance of three different standard-cell-based comparator topologies, considering ultra-low-voltage (ULV) operation. The main application scenarios in which standard-cell-based comparators can be exploited are considered, and a set of figures of merit (FoM) to allow an in-depth comparison among the different topologies is introduced. Then, a set of simulation testbenches are defined in order to simulate and compare the considered topologies implemented in both a 130 nm technology and a 28 nm FDSOI CMOS process. Propagation delay, power consumption and power–delay product are evaluated for different values of the input common mode voltage, as a function of input differential amplitude, and in different supply voltage and temperature conditions. Monte Carlo simulations to evaluate the input offset voltage under mismatch variations are also provided. Simulation results show that the performances of the different comparator topologies are strongly dependent on the input common mode voltage, and that the best values for all the performance figures of merit are achieved by the comparator based on three-input NAND gates, with the only limitation being its non-rail-to-rail input common mode range (ICMR). The performances of the considered comparator topologies have also been simulated for different values of the supply voltage, ranging from 0.3 V to 1.2 V, showing that, even if standard-cell-based comparators can be operated at higher supply voltages by scaling their performances accordingly, the best values of the FoMs are achieved for VDD = 0.3 V.
Moringa oleifera: A Review on the Antiproliferative Potential in Breast Cancer Cells
Authors: Moremane Malebogo M. , Abrahams Beynon , Tiloke Charlette
Published: August 2023

The global burden of female breast cancer and associated deaths has become a major concern. Many chemotherapeutic agents, such as doxorubicin, have been shown to have adverse side effects. The development of multi-drug resistance is a common occurrence, contributing to chemotherapeutic failure. The resistance of breast cancer cells to drug treatment leads to a decline in the treatment efficacy and an increase in cancer recurrence. Therefore, action is required to produce alternative drug therapies, such as herbal drugs. Herbal drugs have been proven to be beneficial in treating illnesses, including cancer. This review aims to highlight the antiproliferative potential of Moringa oleifera (MO), a medicinal tree native to India and indigenous to Africa, in breast cancer cells. Although MO is not yet considered a commercial chemopreventive drug, previous studies have indicated that it could become a chemotherapeutic agent. The possible antiproliferative potential of MO aqueous leaf extract has been previously proven through its antioxidant potential as well as its ability to induce apoptosis. This review will provide an increased understanding of the effect that MO aqueous leaf extract could potentially have against breast cancer.
Development and Evaluation of a New qPCR Assay for the Detection of Mycoplasma in Cell Cultures
Authors: Carrillo-Ávila José A. , de la Fuente Amanda , Aguilar-Quesada Rocío , Ligero Gertrudis , del Río-Ortiz Juan Manuel , Catalina Purificación
Published: August 2023

In recent years, cell culture has become an important tool not only in research laboratories, but also in diagnostic and biotechnological development laboratories. Mycoplasma contamination is present in up to 35% of cell cultures used in research and in cell therapies. This fact represents a significant problem since such contamination can cause disastrous effects on eukaryotic cells by altering their cellular parameters, which, in turn, can lead to unreliable experimental results. For this reason, it is mandatory to carry out continuous testing for the presence of Mycoplasma in cell culture and the development of appropriate methodologies for this purpose. An ideal detection methodology should be fast, sensitive, and reliable. In this study, we propose an alternative detection method based on real-time PCR in conjunction with a novel combination of primers and probes that have been improved to increase their efficiency. The new PCR method demonstrates 100% sensitivity and specificity results in the detection of common Mycoplasma species that contaminate cell cultures. Whilst 11 of 45 tested supernatants were positive for Mycoplasma (24.4%) using the new PCR method (corresponding to 5 of the 14 lines tested (35.71%)), only 10 of 45 supernatants showed positive results with the commercial Venor®GeM qEP and Plasmotest® kit. In addition, the new PCR method exhibits a high capacity to detect less-frequent Mycoplasma species, such as those related to the M. mycoides cluster. The use of an alternative Mycoplasma-detection method in cell culture labs can guarantee the detection of Mycoplasma contamination, especially in cases when dubious results are recorded.
Real-World Analysis of Durvalumab after Chemoradiation in Stage III Non-Small-Cell Lung Cancer
Authors: Preti Beatrice T. B. , Sanatani Michael S. , Breadner Daniel , Lakkunarajah Suganija , Scott Carolyn , Esmonde-White Caroline , McArthur Eric , Rodrigues George , Chaudhary Mitali , Mutsaers Adam , Sachdeva Robin , Vincent Mark D.
Published: August 2023

The 2017 PACIFIC trial heralded the incorporation of routine adjuvant durvalumab following curative-intent chemoradiation for stage III non-small-cell lung cancer (NSCLC). However, carefully selected clinical trial populations can differ significantly from real-world populations, which can have implications on treatment toxicities and outcomes, making it difficult to accurately counsel patients. Consequently, we performed a real-world, retrospective analysis of outcomes and toxicities in 118 patients with stage III NSCLC treated with durvalumab after platinum-based chemoradiotherapy. The data were collected from patients who underwent treatment at a single, tertiary-level Canadian cancer centre from May 2018 to October 2020. The variables collected included patient demographics, treatment specifics, progression-free survival, overall survival, and immune-related adverse events (IRAE) from durvalumab. Descriptive statistics were used for toxicity analysis, and progression-free survival and overall survival estimates were calculated using the Kaplan–Meier method. The statistical analyses indicated a 64.4% (n = 76) toxicity rate, with a 21% (n = 25) toxicity rate of grade 3+ IRAEs. The most common documented IRAEs were pneumonitis (n = 44; 40%), followed by rash (n = 20; 18%) and thyroid dysfunction (n = 17; 15%). FEV1 and DLCO were not found to be associated predictors of pneumonitis toxicity. The median PFS and OS were estimated to be >1.7 years and >2.7 years, respectively.
Microgravity Spherical Droplet Evaporation and Entropy Effects
Authors: Madani Seyedamirhossein , Depcik Christopher
Published: August 2023

Recent efforts to understand low-temperature combustion (LTC) in internal combustion engines highlight the need to improve chemical kinetic mechanisms involved in the negative temperature coefficient (aka cool flame) regime. Interestingly, microgravity droplet combustion experiments demonstrate this cool flame behavior, allowing a greater focus on chemistry after buoyancy, and the corresponding influence of the conservation of momentum is removed. In Experimental terms, the LTC regime is often characterized by a reduction in heat transfer losses. Novel findings in this area demonstrate that lower entropy generation, in conjunction with diminished heat transfer losses, could more definitively define the LTC regime. As a result, the simulation of the entropy equation for spherical droplet combustion under microgravity could help us to investigate fundamental LTC chemical kinetic pathways. To provide a starting point for researchers who are new to this field, this effort first provides a comprehensive and detailed derivation of the conservation of entropy equation using spherical coordinates and gathers all relevant information under one cohesive framework, which is a resource not readily available in the literature. Subsequently, the well-known d2 law analytical model is determined and compared to experimental data that highlight shortcomings of the law. The potential improvements in the d2 law are then discussed, and a numerical model is presented that includes entropy. The resulting codes are available in an online repository to ensure that other researchers interested in expanding this field of work have a fundamental starting point.