bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11
Authors: Tokumoto Shoko , Miyata Yugo , Deviatiiarov Ruslan , Yamada Takahiro G. , Hiki Yusuke , Kozlova Olga , Yoshida Yuki , Cornette Richard , Funahashi Akira , Shagimardanova Elena , Gusev Oleg , Kikawada Takahiro
Published: May 2021

The Pv11, an insect cell line established from the midge Polypedilum vanderplanki, is capable of extreme hypometabolic desiccation tolerance, so-called anhydrobiosis. We previously discovered that heat shock factor 1 (HSF1) contributes to the acquisition of desiccation tolerance by Pv11 cells, but the mechanistic details have yet to be elucidated. Here, by analyzing the gene expression profiles of newly established HSF1-knockout and -rescue cell lines, we show that HSF1 has a genome-wide effect on gene regulation in Pv11. The HSF1-knockout cells exhibit a reduced desiccation survival rate, but this is completely restored in HSF1-rescue cells. By comparing mRNA profiles of the two cell lines, we reveal that HSF1 induces anhydrobiosis-related genes, especially genes encoding late embryogenesis abundant proteins and thioredoxins, but represses a group of genes involved in basal cellular processes, thus promoting an extreme hypometabolism state in the cell. In addition, HSF1 binding motifs are enriched in the promoters of anhydrobiosis-related genes and we demonstrate binding of HSF1 to these promoters by ChIP-qPCR. Thus, HSF1 directly regulates the transcription of anhydrobiosis-related genes and consequently plays a pivotal role in the induction of anhydrobiotic ability in Pv11 cells.
The Immunological Role of the Placenta in SARS-CoV-2 Infection—Viral Transmission, Immune Regulation, and Lactoferrin Activity
Authors: Bukowska-Ośko Iwona , Popiel Marta , Kowalczyk Paweł
Published: May 2021

A pandemic of acute respiratory infections, due to a new type of coronavirus, can cause Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) and has created the need for a better understanding of the clinical, epidemiological, and pathological features of COVID-19, especially in high-risk groups, such as pregnant women. Viral infections in pregnant women may have a much more severe course, and result in an increase in the rate of complications, including spontaneous abortion, stillbirth, and premature birth—which may cause long-term consequences in the offspring. In this review, we focus on the mother-fetal-placenta interface and its role in the potential transmission of SARS-CoV-2, including expression of viral receptors and proteases, placental pathology, and the presence of the virus in neonatal tissues and fluids. This review summarizes the current knowledge on the anti-viral activity of lactoferrin during viral infection in pregnant women, analyzes its role in the pathogenicity of pandemic virus particles, and describes the potential evidence for placental blocking/limiting of the transmission of the virus.
Autophagy Modulators in Cancer Therapy
Authors: Buzun Kamila , Gornowicz Agnieszka , Lesyk Roman , Bielawski Krzysztof , Bielawska Anna
Published: May 2021

Autophagy is a process of self-degradation that plays an important role in removing damaged proteins, organelles or cellular fragments from the cell. Under stressful conditions such as hypoxia, nutrient deficiency or chemotherapy, this process can also become the strategy for cell survival. Autophagy can be nonselective or selective in removing specific organelles, ribosomes, and protein aggregates, although the complete mechanisms that regulate aspects of selective autophagy are not fully understood. This review summarizes the most recent research into understanding the different types and mechanisms of autophagy. The relationship between apoptosis and autophagy on the level of molecular regulation of the expression of selected proteins such as p53, Bcl-2/Beclin 1, p62, Atg proteins, and caspases was discussed. Intensive studies have revealed a whole range of novel compounds with an anticancer activity that inhibit or activate regulatory pathways involved in autophagy. We focused on the presentation of compounds strongly affecting the autophagy process, with particular emphasis on those that are undergoing clinical and preclinical cancer research. Moreover, the target points, adverse effects and therapeutic schemes of autophagy inhibitors and activators are presented.
Olive Fruit Development and Ripening: Break on through to the “-Omics” Side
Authors: Skodra Christina , Titeli Vaia Styliani , Michailidis Michail , Bazakos Christos , Ganopoulos Ioannis , Molassiotis Athanassios , Tanou Georgia
Published: May 2021

The olive tree (Olea europaea L. subsp. europaea) is the most important perennial crop in the Mediterranean region, producing table olives and oil, both appreciated for their nutraceutical value. Although olive oil quality traits have been extensively studied, much less attention has been paid to olive drupe. Olive drupe ripening is an extremely complex process involving numerous physiological and molecular changes that are unique in this fruit crop species. This review underlines the contribution of “-omics” techniques and of the recent advances in bioinformatics and analytical tools, notably next-generation sequencing and mass spectrometry, for the characterization of the olive ripening syndrome. The usage of high-dimensional datasets, such as transcriptomics, proteomics, and metabolomics, will provide a systematical description of the molecular-specific processes regulating olive fruit development and ripening. However, the incomplete sequence of the O. europaea L. reference genome has largely hampered the utilization of omics tools towards olive drupe research. Due to this disadvantage, the most reported -omics studies on fruit trees concern metabolomics and only a few transcriptomics and proteomics. In this review, up-to-date applications of -omics technologies towards olive drupe biology are addressed, and future perspectives in olive fruit research are highlighted.
Diabetes, Albuminuria and the Kidney—Brain Axis
Authors: Ariton Diana Maria , Jiménez-Balado Joan , Maisterra Olga , Pujadas Francesc , Soler María José , Delgado Pilar
Published: May 2021

Cognitive decline and kidney disease are significant public health problems that share similar characteristics and risk factors. The pathophysiology of the kidney–brain axis is not completely understood, and studies analysing the relationship between the biomarkers of kidney damage and cognitive impairment show different results. This article focuses on the epidemiological and clinical aspects concerning the association of albuminuria, a marker for endothelial dysfunction and microvascular disease, and cognitive impairment in patients with chronic kidney disease, diabetic kidney disease and end-stage kidney disease. Most studies show a positive relationship between albuminuria and cognitive impairment in all groups, but evidence in type 2 diabetes (T2D) patients is limited. We briefly discuss the mechanisms underlying these associations, such as damage to the microvascular circulation, leading to hypoperfusion and blood pressure fluctuations, as well as increased inflammation and oxidative stress, both in the brain and in the kidneys. Further clinical and epidemiological studies developed to understand the interplay between the kidneys and brain diseases will hopefully lead to a reduction in cognitive impairment in these patients.
Impact of Air Pollution on Lung Function among Preadolescent Children in Two Cities in Poland
Authors: Dąbrowiecki Piotr , Adamkiewicz Łukasz , Mucha Dominika , Czechowski Piotr Oskar , Soliński Mateusz , Chciałowski Andrzej , Badyda Artur
Published: May 2021

Ambient air pollution impairs lung development in children, particularly in industrialized areas. The air quality in Zabrze, a city located in the Upper Silesian Industrial Region of Poland, is among the worst in Europe. We compared lung function and the frequency of respiratory or allergic symptoms between children living in Zabrze and those living in Gdynia, a city on the Baltic coast, which has the best long-term air quality in Poland. We enrolled children aged 9–15 years from both cities who were able to perform a spirometry. The following spirometry variables were measured for all participants: forced vital capacity (FVC), forced expiratory volume during the first second of expiration (FEV1), FEV1/FVC index, and peak expiratory flow (PEF). The frequencies of respiratory or allergic symptoms were taken from a survey completed by the participants’ parents. In total, 258 children from Gdynia and 512 children from Zabrze were examined. The mean values of FVC, FEV1, and PEF were significantly greater among children in Gdynia than those reported in Zabrze (p ≤ 0.032), and the frequencies of seasonal rhinorrhea (p = 0.015) or coughing episodes (p = 0.022) were significantly higher in Zabrze than in Gdynia. In conclusion, lung function was significantly impaired in children living in Zabrze, an area which is associated with poor air quality. Strategies to improve air quality in the Silesia region are urgently needed.
Prediction of Intraoperative Fluorescence of Brain Gliomas: Correlation between Tumor Blood Flow and the Fluorescence
Authors: Batalov Artem , Goryaynov Sergey , Zakharova Natalya , Solozhentseva Kristina , Kosyrkova Alexandra , Potapov Alexander , Pronin Igor
Published: May 2021

Introduction: The prediction of the fluorescent effect of 5-aminolevulinic acid (5-ALA) in patients with diffuse gliomas can improve the selection of patients. The degree of enhancement of gliomas has been reported to predict 5-ALA fluorescence, while, at the same time, rarer cases of fluorescence have been described in non-enhancing gliomas. Perfusion studies, in particular arterial spin labeling perfusion, have demonstrated high efficiency in determining the degree of malignancy of brain gliomas and may be better for predicting fluorescence than contrast enhancement. The aim of the study was to investigate the relationship between tumor blood flow, measured by ASL, and intraoperative fluorescent glow of gliomas of different grades. Materials and methods: Tumoral blood flow was assessed in 75 patients by pCASL (pseudo-continuous arterial spin labeling) within 1 week prior to surgery. In all cases of tumor removal, 5-ALA had been administered preoperatively. Maximum values of tumoral blood flow (TBF max) were measured, and normalized tumor blood flow (nTBF) was calculated. Results: A total of 76% of patients had significant contrast enhancement, while 24% were non-enhancing. The histopathology revealed 17 WHO grade II gliomas, 12 WHO grade III gliomas and 46 glioblastomas. Overall, there was a relationship between the degree of intraoperative tumor fluorescence and ASL-TBF (Rs = 0.28, p = 0.02 or the TBF; Rs = 0.34, p = 0.003 for nTBF). Non-enhancing gliomas were fluorescent in 9/18 patients, with nTBF in fluorescent gliomas being 54.58 ± 32.34 mL/100 mg/s and in non-fluorescent gliomas being 52.99 ± 53.61 mL/100 g/s (p > 0.05). Enhancing gliomas were fluorescent in 53/57 patients, with nTBF being 170.17 ± 107.65 mL/100 g/s in fluorescent and 165.52 ± 141.71 in non-fluorescent gliomas (p > 0.05). Conclusion: Tumoral blood flow levels measured by non-contrast ASL perfusion method predict the fluorescence by 5-ALA; however, the additional value beyond contrast enhancement is not clear. ASL is, however, useful in cases with contraindication to contrast.
A Comparison of the Clinical and Radiological Extent of Denosumab (Xgeva®) Related Osteonecrosis of the Jaw: A Retrospective Study
Authors: Assili Zineb , Dolivet Gilles , Salleron Julia , Griffaton-Tallandier Claire , Egloff-Juras Claire , Phulpin Bérengère
Published: May 2021

Medication-related osteonecrosis of the jaw (MRONJ) is a severe side effect of antiresorptive medication. The aim of this study was to evaluate the incidence of denosumab-related osteonecrosis of the jaw and to compare the clinical and radiological extent of osteonecrosis. A retrospective study of patients who received Xgeva® at the Institut de Cancérologie de Lorraine (ICL) was performed. Patients for whom clinical and radiological (CBCT) data were available were divided into two groups: “exposed” for patients with bone exposure and “fistula” when only a fistula through which the bone could be probed was observed. The difference between clinical and radiological extent was assessed. The p-value was set at 0.05, and a total of 246 patients were included. The cumulative incidence of osteonecrosis was 0.9% at 6 months, 7% at 12 months, and 15% from 24 months. The clinical extent of MRONJ was significantly less than their radiological extent: in the “exposed” group, 17 areas (45%) were less extensive clinically than radiologically (p < 0.001) and respectively 6 (67%) for the “fistula” group (p < 0.031). It would seem that a CBCT is essential to know the real extent of MRONJ. Thus, it would seem interesting to systematically perform a CBCT during the diagnosis of MRONJ, exploring the entire affected dental arch.
Invasive Fungal Infections in Persons Living with HIV in an Amazonian Context: French Guiana, 2009–2019
Authors: Cachera Laurène , Adenis Antoine , Guarmit Basma , Rabier Sébastien , Couppié Pierre , Djossou Felix , Epelboin Loïc , Melzani Alessia , Abboud Philippe , Blanchet Denis , Demar Magalie , Alsibai Kinan Drak , Nacher Mathieu
Published: May 2021

Although the burden of histoplasmosis in patients with advanced HIV has been the focus of detailed estimations, knowledge about invasive fungal infections in patients living with HIV in an Amazonian context is somewhat scattered. Our goal was thus to adopt a broader view integrating all invasive fungal infections diagnosed over a decade in French Guiana. All patients hospitalized at Cayenne hospital from 1 January 2009 to 31 December 2018 with a proven diagnosis of invasive fungal infection were included (N = 227). Histoplasmosis was the most common (48.2%), followed by Cryptococcus infection (26.3%), and pneumocystosis (12.5%). For cryptococcal infection, there was a discordance between the actual diagnosis of cryptococcal meningitis n = (26) and the isolated presence of antigen in the serum (n = 46). Among the latter when the information was available (n = 34), 21(65.6%) were treated with antifungals but not coded as cryptococcocosis. Most fungal infections were simultaneous to the discovery of HIV (38%) and were the AIDS-defining event (66%). The proportion of major invasive fungal infections appeared to remain stable over the course of the study, with a clear predominance of documented H. capsulatum infections. Until now, the focus of attention has been histoplasmosis, but such attention should not overshadow other less-studied invasive fungal infections.
Successful Treatment of Cryptococcal Meningitis and Cryptococcoma with Isavuconazole in a Patient Living with HIV
Authors: O’Kelly Brendan , Mohamed Aia , Bergin Colm , Lyons Fiona , Rogers Thomas R. , O’Connell Brian , Devitt Emma
Published: May 2021

We describe the successful use of isavuconazole for treatment of an HIV-positive patient with cryptococcal meningitis following induction therapy with liposomal amphotericin B and flucytosine. Because the Cryptococcus neoformans isolate from cerebrospinal fluid had a borderline minimum inhibitory concentration of 8 mg/L, initial consolidation therapy was given with a daily dose of fluconazole 1200 mg based on area under the curve to minimum inhibitory concentration modelling data. Toxicity, and the radiological emergence of a cryptococcoma in the setting of immune reconstitution inflammatory syndrome, prompted a therapeutic switch to isavuconazole. Subsequent imaging after 19 weeks of isavuconazole shows a significant reduction in cryptococcoma size from 11 mm to complete resolution. The patient remains well after 210 days of therapy with a view to completion of treatment after 1 year.
COVID-19 Infection during Pregnancy: Risk of Vertical Transmission, Fetal, and Neonatal Outcomes
Authors: Saadaoui Marwa , Kumar Manoj , Al Khodor Souhaila
Published: May 2021

The COVID-19 pandemic is a worldwide, critical public health challenge and is considered one of the most communicable diseases that the world had faced so far. Response and symptoms associated with COVID-19 vary between the different cases recorded, but it is amply described that symptoms become more aggressive in subjects with a weaker immune system. This includes older subjects, patients with chronic diseases, patients with immunosuppression treatment, and pregnant women. Pregnant women are receiving more attention not only because of their altered physiological and immunological function but also for the potential risk of viral vertical transmission to the fetus or infant. However, very limited data about the impact of maternal infection during pregnancy, such as the possibility of vertical transmission in utero, during birth, or via breastfeeding, is available. Moreover, the impact of infection on the newborn in the short and long term remains poorly understood. Therefore, it is vital to collect and analyze data from pregnant women infected with COVID-19 to understand the viral pathophysiology during pregnancy and its effects on the offspring. In this article, we review the current knowledge about pre-and post-natal COVID-19 infection, and we discuss whether vertical transmission takes place in pregnant women infected with the virus and what are the current recommendations that pregnant women should follow in order to be protected from the virus.
Different Associations between Tonsil Microbiome, Chronic Tonsillitis, and Intermittent Hypoxemia among Obstructive Sleep Apnea Children of Different Weight Status: A Pilot Case-Control Study
Authors: Chuang Hai-Hua , Hsu Jen-Fu , Chuang Li-Pang , Chiu Cheng-Hsun , Huang Yen-Lin , Li Hsueh-Yu , Chen Ning-Hung , Huang Yu-Shu , Chuang Chun-Wei , Huang Chung-Guei , Lai Hsin-Chih , Lee Li-Ang
Published: May 2021

The tonsil microbiome is associated with chronic tonsillitis and obstructive sleep apnea (OSA) in children, and the gut microbiome is associated with host weight status. In this study, we hypothesized that weight status may be associated with clinical profiles and the tonsil microbiome in children with OSA. We prospectively enrolled 33 non-healthy-weight (cases) and 33 healthy-weight (controls) pediatric OSA patients matched by the proportion of chronic tonsillitis. Differences in the tonsil microbiome between the non-healthy-weight and healthy-weight subgroups and relationships between the tonsil microbiome and clinical variables were investigated. Non-healthy weight was associated with significant intermittent hypoxemia (oxygen desaturation index, mean blood saturation (SpO2), and minimal SpO2) and higher systolic blood pressure percentile, but was not related to the tonsil microbiome. However, chronic tonsillitis was related to Acidobacteria in the non-healthy-weight subgroup, and oxygen desaturation index was associated with Bacteroidetes in the healthy-weight subgroup. In post hoc analysis, the children with mean SpO2 ≤ 97% had reduced α and β diversities and a higher abundance of Bacteroidetes than those with mean SpO2 > 97%. These preliminary findings are novel and provide insights into future research to understand the pathogenesis of the disease and develop personalized treatments for pediatric OSA.
Spatiotemporal Differentiation of Territorial Space Development Intensity and Its Habitat Quality Response in Northeast China
Authors: Yang Zhipeng , Wang Shijun , Guo Meng , Tian Junfeng , Zhang Yingjie
Published: May 2021

Studying the spatiotemporal distribution characteristics of territorial space development intensity (TSDI) and its habitat quality (HQ) response is of substantial theoretical and practical significance to optimize regional development patterns and coordinate the relationship between territorial space development and eco-environmental protection. This study establishes a comprehensive assessment model across various aspects, including land, population, economy, and input, to assess the TSDI of each county in Northeast China. We used the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model to evaluate the HQ of each county and investigated the HQ response to TSDI. The results showed that the TSDI in Northeast China was high in the south and west, low in the north and east, and prominent in urban agglomeration areas, which increased between 2000 and 2015. The spatial pattern of HQ was low in the east and south, high in the west and north, and the HQ was degraded as a whole. Bivariate spatial autocorrelation analysis showed a significant negative spatial correlation between TSDI and HQ, and distinct patterns of local spatial agglomeration were identified. Our findings provide guidelines for territorial space planning and may offer a reference for the ecological civilization construction and the coordinated development of Northeast China.
Mechanical Properties of a Chiral Cellular Structure with Semicircular Beams
Authors: Bai Yalei , Zhao Tong , Yuan Chengxu , Liu Weidong , Zhang Haichao , Yang Lei , She Chongmin
Published: May 2021

Compliant cellular structures are good candidates for morphing applications. This paper proposes a novel chiral cellular structure composed of circular beams with great elastic properties and potential for morphing. The tensile and shear elastic properties of the structure are studied through theoretical derivations and then verified by finite element analysis. Results show that this novel chiral structure exhibits extremely low in-plane tensile and shear moduli, which are many orders of magnitude lower than that of the raw material. The structure also shows tensile–shear and shear–tensile coupling effects that cannot be ignored. The tensile and shear properties of the structure can provide a reference for employing this structure in engineering applications.
A Product Pose Tracking Paradigm Based on Deep Points Detection
Authors: Bampis Loukas , Mouroutsos Spyridon G. , Gasteratos Antonios
Published: May 2021

The paper at hand presents a novel and versatile method for tracking the pose of varying products during their manufacturing procedure. By using modern Deep Neural Network techniques based on Attention models, the most representative points to track an object can be automatically identified using its drawing. Then, during manufacturing, the body of the product is processed with Aluminum Oxide on those points, which is unobtrusive in the visible spectrum, but easily distinguishable from infrared cameras. Our proposal allows for the inclusion of Artificial Intelligence in Computer-Aided Manufacturing to assist the autonomous control of robotic handlers.
Fish Sidestream-Derived Protein Hydrolysates Suppress DSS-Induced Colitis by Modulating Intestinal Inflammation in Mice
Authors: Daskalaki Maria G. , Axarlis Konstantinos , Aspevik Tone , Orfanakis Michail , Kolliniati Ourania , Lapi Ioanna , Tzardi Maria , Dermitzaki Eirini , Venihaki Maria , Kousoulaki Katerina , Tsatsanis Christos
Published: May 2021

Inflammatory bowel disease is characterized by extensive intestinal inflammation, and therapies against the disease target suppression of the inflammatory cascade. Nutrition has been closely linked to the development and suppression of inflammatory bowel disease, which to a large extent is attributed to the complex immunomodulatory properties of nutrients. Diets containing fish have been suggested to promote health and suppress inflammatory diseases. Even though most of the health-promoting properties of fish-derived nutrients are attributed to fish oil, the potential health-promoting properties of fish protein have not been investigated. Fish sidestreams contain large amounts of proteins, currently unexploited, with potential anti-inflammatory properties, and may possess additional benefits through bioactive peptides and free amino acids. In this project, we utilized fish protein hydrolysates, based on mackerel and salmon heads and backbones, as well as flounder skin collagen. Mice fed with a diet supplemented with different fish sidestream-derived protein hydrolysates (5% w/w) were exposed to the model of DSS-induced colitis. The results show that dietary supplements containing protein hydrolysates from salmon heads suppressed chemically-induced colitis development as determined by colon length and pro-inflammatory cytokine production. To evaluate colitis severity, we measured the expression of different pro-inflammatory cytokines and chemokines and found that the same supplement suppressed the pro-inflammatory cytokines IL-6 and TNFα and the chemokines Cxcl1 and Ccl3. We also assessed the levels of the anti-inflammatory cytokines IL-10 and Tgfb and found that selected protein hydrolysates induced their expression. Our findings demonstrate that protein hydrolysates derived from fish sidestreams possess anti-inflammatory properties in the model of DSS-induced colitis, providing a novel underexplored source of health-promoting dietary supplements.
Year-Round Cultivation of Tetraselmis sp. for Essential Lipid Production in a Semi-Open Raceway System
Authors: Lee Won-Kyu , Ryu Yong-Kyun , Choi Woon-Yong , Kim Taeho , Park Areumi , Lee Yeon-Ji , Jeong Younsik , Lee Choul-Gyun , Kang Do-Hyung
Published: May 2021

There is increasing demand for essential fatty acids (EFAs) from non-fish sources such as microalgae, which are considered a renewable and sustainable biomass. The open raceway system (ORS) is an affordable system for microalgae biomass cultivation for industrial applications. However, seasonal variations in weather can affect biomass productivity and the quality of microalgal biomass. The aim of this study was to determine the feasibility of year-round Tetraselmis sp. cultivation in a semi-ORS in Korea for biomass and bioactive lipid production. To maximize biomass productivity of Tetraselmis sp., f medium was selected because it resulted in a significantly higher biomass productivity (1.64 ± 0.03 g/L) and lower omega-6/omega-3 ratio (0.52/1) under laboratory conditions than f/2 medium (0.70/1). Then, we used climatic data-based building information modeling technology to construct a pilot plant of six semi-ORSs for controlling culture conditions, each with a culture volume of 40,000 L. Over 1 year, there were no significant variations in monthly biomass productivity, fatty acid composition, or the omega-6/omega-3 ratio; however, the lipid content correlated significantly with photosynthetic photon flux density. During year-round cultivation from November 2014 to October 2017, areal productivity was gradually increased by increasing medium salinity and injecting CO2 gas into the culture medium. Productivity peaked at 44.01 g/m2/d in October 2017. Throughout the trials, there were no significant differences in average lipid content, which was 14.88 ± 1.26%, 14.73 ± 2.44%, 12.81 ± 2.82%, and 13.63 ± 3.42% in 2014, 2015, 2016, and 2017, respectively. Our results demonstrated that high biomass productivity and constant lipid content can be sustainably maintained under Korean climate conditions.
Isolation and Characterization of Beneficial Bacteria from Food Process Wastes
Authors: Kim A-Leum , Park Seunghye , Hong Yoon-Kyoung , Shin Ji-Hwan , Joo Se-Hwan
Published: May 2021

Significant quantities of food waste are accumulated globally on an annual basis, with approximately one-third of the food produced (equivalent to 1.3 billion tons of food) being wasted each year. A potential food waste recycling application is its utilization as a soil conditioner or fertilizer, whereby it increases the soil organic content and microbial biomass. This study evaluated the effectiveness of food waste as a microbial resource by analyzing the microbial community composition and isolating plant growth-promoting bacteria (PGPB) in food waste obtained from various sources. High-throughput sequencing identified 393 bacterial operational taxonomic units in the food process waste (FPW) samples. Moreover, the results showed that Firmicutes was abundant in the waste samples, followed by Bacteroidetes and Proteobacteria. A total of 92 bacteria were isolated from FPW. Moreover, the cultivable strains isolated from FPW belonged to the genus Bacillus, followed by Streptomyces and Proteus. Six isolated bacteria exhibited beneficial traits, including indole acetic acid production, antifungal resistance and extracellular lysis. FPW is a valuable microbial resource for isolation of PGPB, and its use as a fertilizer may enable a reduction in chemical fertilizer usage, thereby mitigating the corresponding adverse environmental impacts on sustainable crop development.
Tracing Pre-Mesozoic Tectonic Sutures in the Crystalline Basement of the Protocarpathians: Evidence from the Exotic Blocks from Subsilesian Nappe, Outer Western Carpathians, Poland
Authors: Golonka Jan , Gawęda Aleksandra , Waśkowska Anna , Chew David , Szopa Krzysztof , Drakou Foteini
Published: May 2021

Pre-Mesozoic exotic crystalline blocks within the Outer Carpathian flysch have potential to unravel the nature of their eroded basement source(s) and to reconstruct the Paleozoic–Precambrian history of the Protocarpathians. Strongly tectonized Campanian–Maastrichtian grey marls in the Subsilesian Nappe of the Outer Western Carpathians in Poland contain a variety of different lithology types, including granitoids and andesites. Petrological investigations coupled with zircon and apatite U-Pb dating were performed on crystalline (subvolcanic) exotic blocks from a locality in the Subsilesian Nappe. U-Pb zircon dating yields magmatic crystallization ages of c. 293 Ma for the microgranitoid and c. 310 Ma for the andesite block, with inherited zircon cores yielding Archean, Paleoproterozoic, Mesoproterozoic and Cadomian ages. Whole rock trace element and Nd isotope data imply that the melt source was composed of a significant Neoproterozoic crustal component in both the microgranite and andesite. The Late Carboniferous–Permian magmatic activity likely continues outside the Carpathian Belt and can be linked to a Late Paleozoic transtensional zone, which is a continuation of the Lubliniec–Kraków Zone that extends under the Carpathians to Moesia. This Late Paleozoic transtensional zone was probably reactivated during the Late Cretaceous under a transpressional regime within the Żegocina tectonic zone, which caused the uplift of the Subsilesian Ridge and intensive erosion.
Effects of Rice Straw Powder (RSP) Size and Pretreatment on Properties of FDM 3D-Printed RSP/Poly(lactic acid) Biocomposites
Authors: Yu Wangwang , Dong Lili , Lei Wen , Zhou Yuhan , Pu Yongzhe , Zhang Xi
Published: May 2021

To develop a new kind of environment-friendly composite filament for fused deposition modeling (FDM) 3D printing, rice straw powder (RSP)/poly(lactic acid) (PLA) biocomposites were FDM-3D-printed, and the effects of the particle size and pretreatment of RSP on the properties of RSP/PLA biocomposites were investigated. The results indicated that the 120-mesh RSP/PLA biocomposites (named 120#RSP/PLA) showed better performance than RSP/PLA biocomposites prepared with other RSP sizes. Infrared results showed that pretreatment of RSP by different methods was successful, and scanning electron microscopy indicated that composites prepared after pretreatment exhibited good interfacial compatibility due to a preferable binding force between fiber and matrix. When RSP was synergistically pretreated by alkaline and ultrasound, the composite exhibited a high tensile strength, tensile modulus, flexural strength, and flexural modulus of 58.59, 568.68, 90.32, and 3218.12 MPa, respectively, reflecting an increase of 31.19%, 16.48%, 18.75%, and 25.27%, respectively, compared with unmodified 120#RSP/PLA. Pretreatment of RSP also improved the thermal stability and hydrophobic properties, while reducing the water absorption of 120#RSP/PLA. This work is believed to provide highlights of the development of cost-effective biocomposite filaments and improvement of the properties of FDM parts.
Spent Grain from Malt Whisky: Assessment of the Phenolic Compounds
Authors: Chetrariu Ancuța , Dabija Adriana
Published: May 2021

In order to extract antioxidant phenolic compounds from spent grain (SG) two extraction methods were studied: the ultrasound-assisted method (US) and the Ultra-Turrax method (high stirring rate) (UT). Liquid to solid ratios, solvent concentration, time, and temperature/stirring rate were optimized. Spent grain extracts were analyzed for their total phenol content (TPC) (0.62 to 1.76 mg GAE/g SG DW for Ultra-Turrax pretreatment, and 0.57 to 2.11 mg GAE/g SG DW for ultrasound-assisted pretreatment), total flavonoid content (TFC) (0.6 to 1.67 mg QE/g SG DW for UT, and 0.5 to 1.63 mg QE/g SG DW for US), and antioxidant activity was measured using 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical (25.88% to 79.58% for UT, and 27.49% to 78.30% for UT). TPC was greater at a high stirring rate and high exposure time up to a certain extent for the Ultra-Turrax method, and at a high temperature for the ultrasound-assisted method. P-coumaric acid (20.4 ± 1.72 mg/100 SG DW for UT, and 14.0 ± 1.14 mg/100 SG DW for US) accounted for the majority of the phenolic found compounds, followed by rosmarinic (6.5 ± 0.96 mg/100 SG DW for UT, and 4.0 ± 0.76 mg/100 SG DW for US), chlorogenic (5.4 ± 1.1 mg/100 SG DW for UT, and non-detectable for US), and vanillic acids (3.1 ± 0.8 mg/100 SG DW for UT, and 10.0 ± 1.03 mg/100 SG DW for US) were found in lower quantities. Protocatechuic (0.7 ± 0.05 mg/100 SG DW for UT, and non-detectable for US), 4-hydroxy benzoic (1.1 ± 0.06 mg/100 SG DW for UT, and non-detectable for US), and caffeic acids (0.7 ± 0.03 mg/100 SG DW for UT, and non-detectable for US) were present in very small amounts. Ultrasound-assisted and Ultra-Turrax pretreatments were demonstrated to be efficient methods to recover these value-added compounds.
Investigating Sex Differences in Rates and Correlates of Food Addiction Status in Women and Men with PTSD
Authors: Stojek Monika M. , Lipka Justyna , Maples-Keller Jessica M. , Rauch Sheila A. M. , Black Kathryn , Michopoulos Vasiliki , Rothbaum Barbara O.
Published: May 2021

Background: Food addiction (FA) is a dysregulated eating pattern characterized by difficulties in controlling the intake of certain foods. There is an overlap in physical and mental health correlates of FA and post-traumatic stress disorder (PTSD). The purpose of this study was to examine sex differences in the rates of positive FA status in individuals with threshold/subthreshold PTSD, and to examine sex differences in the physical and mental health correlates of FA. Methods: Post-9/11 veterans/service members seeking PTSD treatment were recruited. Participants were diagnosed with PTSD via the administration of a clinical interview. FA status was determined using Modified Yale Food Addiction Scale-2, binary sex and body mass index were assessed with demographics questions. Results: Nearly half (43%) of the sample were women. There were no sex differences in the rates of FA, with an overall FA prevalence of 18%. There were no sex differences in FA symptom count in the whole sample (M = 1.63) or those with FA status (M = 6.21). Individuals with FA reported higher frequency of disordered eating, higher severity of PTSD, and depression symptoms. Conclusions: FA should be assessed in tandem with PTSD symptoms, as its prevalence in that sample is higher than in the general population, and it appears to affect both sexes at similar rates.
Comparison of Solution Chemical Properties and Biological Activity of Ruthenium Complexes of Selected β-Diketone, 8-Hydroxyquinoline and Pyrithione Ligands
Authors: Pivarcsik Tamás , Tóth Gábor , Szemerédi Nikoletta , Bogdanov Anita , Spengler Gabriella , Kljun Jakob , Kladnik Jerneja , Turel Iztok , Enyedy Éva A.
Published: May 2021

In this work, the various biological activities of eight organoruthenium(II) complexes were evaluated to reveal correlations with their stability and reactivity in aqueous media. Complexes with general formula [Ru(η6-p-cymene)(X,Y)(Z)] were prepared, where (X,Y) represents either an O,O-ligand (β-diketone), N,O-ligand (8-hydroxyquinoline) or O,S-pyrithione-type ligands (pyrithione = 1-hydroxypyridine-2(1H)-thione) with Cl− or 1,3,5-triaza-7-phosphaadamantane (PTA) as a co-ligand (Z). The tested complexes inhibit the chlamydial growth on HeLa cells, and one of the complexes inhibits the growth of the human herpes simplex virus-2. The chlorido complexes with N,O- and O,S-ligands displayed strong antibacterial activity on Gram-positive strains including the resistant S. aureus (MRSA) and were cytotoxic in adenocarcinoma cell lines. Effect of the structural variation on the biological properties and solution stability was clearly revealed. The decreased bioactivity of the β-diketone complexes can be related to their lower stability in solution. In contrast, the O,S-pyrithione-type complexes are highly stable in solution and the complexation prevents the oxidation of the O,S-ligands. Comparing the binding of PTA and the chlorido co-ligands, it can be concluded that PTA is generally more strongly coordinated to ruthenium, which at the same time decreased the reactivity of complexes with human serum albumin or 1-methylimidazole as well as diminished their bioactivity.
Analysis and Simulation of Polymer Injectivity Test in a High Temperature High Salinity Carbonate Reservoir
Authors: Alzaabi Mohamed Adel , Leon Juan Manuel , Skauge Arne , Masalmeh Shehadeh
Published: May 2021

Polymer flooding has gained much interest within the oil industry in the past few decades as one of the most successful chemical enhanced oil recovery (CEOR) methods. The injectivity of polymer solutions in porous media is a key factor in polymer flooding projects. The main challenge that faces prediction of polymer injectivity in field applications is the inherent non-Newtonian behavior of polymer solutions. Polymer in situ rheology in porous media may exhibit complex behavior that encompasses shear thickening at high flow rates in addition to the typical shear thinning at low rates. This shear-dependent behavior is usually measured in lab core flood experiments. However, data from field applications are usually limited to the well bottom-hole pressure (BHP) as the sole source of information. In this paper, we analyze BHP data from field polymer injectivity test conducted in a Middle Eastern heterogeneous carbonate reservoir characterized by high-temperature and high-salinity (HTHS) conditions. The analysis involved incorporating available data to build a single-well model to simulate the injectivity test. Several generic sensitivities were tested to investigate the impact of stepwise variation in injection flow rate and polymer concentration. Polymer injection was reflected in a non-linear increase in pressure with injection, and longer transient behavior toward steady state. The results differ from water injection which have linear pressure response to rate variation, and quick stabilization of pressure after rate change. The best match of the polymer injection was obtained with complex rheology, that means the combined shear thickening at high rate near the well and moving through apparent Newtonian and shear thinning at low rate.
Experimental, Computational, and Dimensional Analysis of the Mechanical Performance of Fused Filament Fabrication Parts
Authors: Rivet Iván , Dialami Narges , Cervera Miguel , Chiumenti Michele , Reyes Guillermo , Pérez Marco A.
Published: May 2021

Process parameters in Additive Manufacturing (AM) are key factors in the mechanical performance of 3D-printed parts. In order to study their effect, a three-zone model based on the printing pattern was developed. This modelization distinguished three different zones of the 3D-printed part, namely cover, contour, and inner; each zone was treated as a different material. The cover and contour zones were characterized via uniaxial tensile tests and the inner zones via computational homogenization. The model was then validated by means of bending tests and their corresponding computational simulations. To reduce the number of required characterization experiments, a relationship between the raw and 3D-printed material was established by dimensional analysis. This allowed describing the mechanical properties of the printed part with a reduced set of the most influential non-dimensional relationships. The influence on the performance of the parts of inter-layer adhesion was also addressed in this work via the characterization of samples made of Polycarbonate Acrylonitrile Butadiene Styrene (ABS/PC), a polymeric material well known for its poor adhesion strength. It was concluded that by using this approach, the number of required testing configurations could be reduced by two thirds, which implies considerable cost savings.
Novel 3D Imaging Systems for High-Throughput Phenotyping of Plants
Authors: Gao Tian , Zhu Feiyu , Paul Puneet , Sandhu Jaspreet , Doku Henry Akrofi , Sun Jianxin , Pan Yu , Staswick Paul , Walia Harkamal , Yu Hongfeng
Published: May 2021

The use of 3D plant models for high-throughput phenotyping is increasingly becoming a preferred method for many plant science researchers. Numerous camera-based imaging systems and reconstruction algorithms have been developed for the 3D reconstruction of plants. However, it is still challenging to build an imaging system with high-quality results at a low cost. Useful comparative information for existing imaging systems and their improvements is also limited, making it challenging for researchers to make data-based selections. The objective of this study is to explore the possible solutions to address these issues. We introduce two novel systems for plants of various sizes, as well as a pipeline to generate high-quality 3D point clouds and meshes. The higher accuracy and efficiency of the proposed systems make it a potentially valuable tool for enhancing high-throughput phenotyping by integrating 3D traits for increased resolution and measuring traits that are not amenable to 2D imaging approaches. The study shows that the phenotype traits derived from the 3D models are highly correlated with manually measured phenotypic traits (R2 > 0.91). Moreover, we present a systematic analysis of different settings of the imaging systems and a comparison with the traditional system, which provide recommendations for plant scientists to improve the accuracy of 3D construction. In summary, our proposed imaging systems are suggested for 3D reconstruction of plants. Moreover, the analysis results of the different settings in this paper can be used for designing new customized imaging systems and improving their accuracy.
Olanzapine Loaded Nanostructured Lipid Carriers via High Shear Homogenization and Ultrasonication
Authors: Ajiboye Adejumoke Lara , Nandi Uttom , Galli Martin , Trivedi Vivek
Published: May 2021

The aim of this study was to understand the effect of high shear homogenization (HSH) and ultrasonication (US) on the physicochemical properties of blank and olanzapine loaded nanostructured lipid carriers (NLCs) along with their drug loading potential and drug release profiles from formulated particles. NLCs were prepared with different ratios of Compritol and Miglyol as the solid and liquid lipids, respectively, under changing HSH and US times between 0 to 15 min. The surfactants (Poloxamer 188 (P188) and tween 80) and the drug content was kept constant in all formulations. The prepared NLCs were evaluated for particle size, polydispersity index, zeta potential, drug crystallinity and chemical interactions between lipids and OLZ. The in-vitro drug release was performed using dialysis tube method in phosphate buffer solution (PBS) at pH 7.4. The formulated NLCs were negatively charged, spherically shaped and monodisperse, with particle sizes ranging from 112 to 191 nm. There was a significant influence of US time on the preparation of NLCs in comparison to HSH, where a significant reduction in the mean particle diameter was seen after 5 min of sonication. An increase of Miglyol content in NLCs led to an increase in particle size. In general, application of US led to decrease in particle size after HSH but an increase in particle diameter of low Miglyol containing preparation was also observed with longer sonication time. OLZ was successfully encapsulated in the NLCs and a total release of 89% was achieved in 24 h in PBS at pH 7.4.
Performance Assessment for Short-Term Water Demand Forecasting Models on Distinctive Water Uses in Korea
Authors: Koo Kang-Min , Han Kuk-Heon , Jun Kyung-Soo , Lee Gyumin , Kim Jung-Sik , Yum Kyung-Taek
Published: May 2021

It is crucial to forecast the water demand accurately for supplying water efficiently and stably in a water supply system. In particular, accurately forecasting short-term water demand helps in saving energy and reducing operating costs. With the introduction of the Smart Water Grid (SWG) in a water supply system, the amount of water consumption is obtained in real-time through a smart meter, which can be used for forecasting the short-term water demand. The models widely used for water demand forecasting include Autoregressive Integrated Moving Average, Radial Basis Function-Artificial Neural Network, Quantitative Multi-Model Predictor Plus, and Long Short-Term Memory. However, there is a lack of research on assessing the performance of models and forecasting the short-term water demand in the SWG demonstration plant. Therefore, in this study, the short-term water demand was forecasted for each model using the data collected from a smart meter, and the performance of each model was assessed. The Smart Water Grid Research Group installed a smart meter in block 112 located in YeongJong Island, Incheon, and the actual data used for operating the SWG demonstration plant were adopted. The performance of the model was assessed by using the Residual, Root Mean Square Error, Normalized Root Mean Square Error, Nash–Sutcliffe Efficiency, and Pearson Correlation Coefficient as indices. As a result of water demand forecasting, it is difficult to forecast water demand only by time and water consumption. Therefore, as the short-term water demand forecasting models using only time and the amount of water consumption have limitations in reflecting the characteristics of consumers, a water supply system can be managed more precisely if other factors (weather, customer behavior, etc.) influencing the water demand are applied.