bluuebunny/crossref_metadata_2025 huggingface dataset:
Strategi Misi William Carey dan Implikasinya di Era New Normal
Authors: Ijen Ijen , Lisa Maria Oxtavia
Published: September 2023

Strategi misi William Carey merupakan salah satu strategi misi yang berdampak di India. Strategi misi William Carey tampaknya masih relevan dalam konteks masa kini. William Carey mengabdikan diri untuk pergi ke India dan meninggalkan kampung halamannya. Tentu ada suasana, kondisi, dan keadaan baru yang dihadapinya. Hal itu selaras dengan kondisi di Era New Normal. Era New Normal merupakan kondisi tidak seperti biasanya atau keadaan yang baru. Oleh sebab itu, penelitian ini dilaksanakan untuk menemukan strategi misi William Carey dan implikasinya di era new normal. Penelitian dilaksanakan dengan metode penelitian pustaka. Berdasarkan hasil penelitian ditemukan bahwa strategi misi William Carey adalah membuka atau mendirikan sekolah, membuka lembaga misi, menghargai budaya, dan menerjemahkan Alkitab. Dan implikasi di era new normal yaitu melakukan pengajaran secara virtual, memberikan pelatihan misi lewat youtube, mengikuti perkembangan zaman, dan membuat aplikasi penerjemahan Alkitab ke bahasa daerah.
Pendekatan Penginjilan Kontekstual Terhadap Budaya Pekan Gawai Suku Dayak
Authors: Akanpeni Trisilpa , Saputra Roland Holy , Ina Ernesta Tamo , Tansi Filmon Gusti
Published: September 2023

This study analyzes the culture of Gawai Week to assist in finding cultural meaning and assisting in the evangelistic approach to gawai week culture. Through this gadget week, the Dayak tribe can gather to hold various attractions as well as socialize in it. This custom is carried out once a year involving the entire Dayak community. The custom of this gawai week still believes in the abundance of blessings received from the ancestors. Through this habit, it is used as a contextualized medium for evangelism. By taking a persuasive approach in order to be able to change a good and correct perspective on the blessings received through the gadget week. It is this good perspective that can change perspectives and can also incorporate the Bible into the culture of gadget week to understand where the words are received in gadget week.
Peran Gereja Guna Mengurangi Kasus KDRT dalam Jemaat
Authors: Polyongkico Polyongkico , Nelsen Nelsen
Published: September 2023

Domestic Violence (KDRT) can happen anywhere and to anyone, including the congregation. The research objectives to be achieved from this study are to describe the role of the church in congregational domestic violence victims and to describe the study of pastoral counseling for victims of domestic violence. The method used in this study is a descriptive method with a qualitative approach. In writing this research the author wants to explain the role of the church in the congregation to help victims of domestic violence, with several forms that the church does, namely: first: paying attention to victims and visiting victims in giving care in the sense of hearing complaints and strengthening and providing input that can help a victim (pastoral counseling). Second: providing assistance in the form of medical treatment and psychological (mental) treatment. So the role of the church is also to help victims of domestic violence that can happen to the congregation, and the church can build a Christian family based on belief in Jesus Christ.
Prinsip Pemuridan Menurut Kisah Para Rasul 2:42 Bagi Pertumbuan Rohani Jemaat Masa Kini
Authors: Hermanto Abang
Published: September 2023

This article discusses the discipleship principle in Acts 2:42 for the spiritual growth of the church today as seen in the output in Acts 2:47. This study aims to explain the principles of discipleship that are relevant for the spiritual growth of the church today, by studying the principles found in Acts. Researchers use qualitative research methods and a biblical approach. This principle focuses on the apostles' teaching, fellowship, breaking bread, and prayer as means of deepening understanding of Christ's doctrine. The guiding principles used by followers of Jesus Christ at that time included several important elements. First, the direct teaching of the apostles became the main foundation of learning. This emphasizes the importance of having teachers who provide proper teaching. Second, fellowship becomes an important element in learning. Followers of Christ learn from others through discussion, brainstorming, and helping each other understand the doctrine of Christ. Third, breaking bread becomes a significant learning method. During this activity, followers of Christ discuss and deepen their understanding of Christ's doctrine. This activity creates strong social bonds between members of the congregation and allows for mutual strengthening in faith.  Fourth, prayer is an important aspect of this learning method. Followers of Christ learn through prayer and strengthen faith through prayer together.
Hubungan Media Sosial Terhadap Krisis Identitas Remaja: Studi Kualitatif
Authors: Rope Denny
Published: September 2023

The development of social media as a digital platform cannot be separated from daily human activities. However, this development not only has a positive impact but also has a negative influence on some people, especially teenagers. All stakeholders, especially families and churches, must have the responsibility and become navigators to direct and fill self-identity in adolescents based on the values of the Word of God. This research seeks to explore the influence of digital platforms, namely social media, on adolescent identity crises. The method used in this research is a quantitative method through distributing questionnaires to teenagers aged 12-17 years from junior high school to high school. The results found that on average these teenagers spend five hours a day connected to social media. The author found that the significant value of using social media such as TikTok, Instagram, Youtube and Facebook on behavior is 0.63> 0.05, meaning it is not significant. Likewise, social media on emotions and trust values.  This research aims to motivate teenagers to use digital platforms properly and beneficially for everyone.
Metode Baru untuk Menghubungkan Peserta Didik dengan Iman Kristen
Authors: Lie Romi
Published: September 2023

Innovation in Christian Religious Education can create a more effective and engaging learning process for students, develop relevant skills and competencies, and increase students' interest and participation in Christian Religious Education. The main purpose of innovation in Christian Religious Education in the digital era is to create or discover methods/approaches that are more relevant and engaging for students in learning about Christianity. The method used in this research is literature review. This research method is used to find new methods to connect students with their faith through innovation in Christian Religious Education in the digital era. The benefits of innovation in Christian Religious Education in the digital era are to increase the effectiveness of learning Christian Religious Education, develop relevant skills and competencies, and increase students' interest and participation in Christian Religious Education. With innovation in Christian Religious Education in the digital era, students can connect with their faith and beliefs more effectively and authentically.
Minimnya Penggunaan Media Sosial Sebagai Sarana Komunikasi Injil oleh Pemuda Kristen di Abad 21
Authors: Tansi Filmon Gusti , Beriang Senan
Published: September 2023

In modern life, especially in the 21st century, the influence of technological developments is very rapid, including the presence of social media (social media) and it has become a basic need for humanity in the technological era and not to forget the church as a place for people to worship. Every congregation within the church environment has known and used social media for various things including facilitating congregations to read the Bible, electronic hymnbooks and so on. However, it seems that there are still some congregations who are less responsive in using social media wisely. Christian youth in the 21st century, who are the majority of active social media users, seem less aware of this opportunity, so they experience difficulties in preaching the Gospel to others. For this reason, the aim of this research is to provide good knowledge so that Christian youth in the 21st century can utilize social media as a means of preaching the Gospel based on the Great Commission. The method used in this scientific work is a qualitative research method, by means of observation, interviews and documentation. The author chose this type of research because the author wanted to find out why there was a lack of response from Christian youth in the 21st century in utilizing social media as a means of preaching the gospel. The results of this scientific work are to raise awareness of Christian youth regarding the importance of using social media in preaching the Gospel.
Tinjauan Teologis Terhadap Fenomena LGBT Bagi Gereja dan Masyarakat Masa Kini
Authors: Putra Adi , Zebua Marta Novianti , Nome Nehemia , Keluanan Yane Henderina
Published: September 2023

This research specifically examines the phenomenon of LGBT in society. The goal is to produce a perspective and an effective way that the Church and society should do to serve and heal LGBT sufferers. This research uses qualitative methods, especially literature review to describe, analyze to produce new propositions on this topic. Through this research, several conclusions were obtained. First, LGBT is a sin and a mistake that arises as a result of sin, so it needs to be addressed with great care and wisdom so that you don't react wrongly. Second, the role of the Church is so significant in serving every person with LGBT. The church must not excommunicate or reject them, but it must not ostensibly justify their every wrong action. On the other hand, the church must firmly say that LGBT is a mistake and a sin that violates God's glory, while the church carries out pastoral actions and services to heal and restore them. Third, apart from the church, the role of the general public is also significant. Because society must help every person with LGBT to realize and want to improve their life that has deviated from God's will.
Ground Variation, Geotechnical Uncertainties and Reliability of Foundation Design for Sustainable Building Infrastructures with Case Histories
Authors:   Centre for Geomechanics, Energy and Environmental Sustainability, Port Harcourt, Nigeria, Abija Fidelis A.
Published: September 2023

The foundation of any civil engineering structure serves to transfer the superstructure load to the ground without causing any uneven response, excessive settlement and movement. The choice and design of any foundation requires consideration for the load, subsoil type, mineralogy and variation; suitable bearing capacity, groundwater conditions, geological and stress history, tolerable settlement and understanding of the genesis and stratigraphy of the site. Uncertainties are signature characteristic of geotechnical paramterization and design. Application of reliability analysis is recommended for choosing the optimal design and construction materials and method.. Therefore a Geotechnical Consultant designate is required throughout the project life cycle to forestall collapse.
LA INNOVACIÓN PEDAGÓGICA: SOSPECHA POR LA INSTALACIÓN DE UN DISCURSO EN EL QUEHACER PEDAGÓGICO DE LOS MAESTROS (1980-2020)
Authors:   Universidad Pedagógica Nacional, Uribe Garzón Sonia Milena , Cárdenas Forero Óscar Leonardo ,   Universidad Pedagógica Nacional
Published: September 2023

In recent decades, the work of Bogotá public school teachers has been conquered by the irruption of the rhetoric of pedagogical innovation. This incursion has become naturalized in the pedagogical practices of teachers, to such an extent that it is inconceivable to think of their absence from pedagogical work, establishing itself as a "truth policy" to guide their modes of action in the classroom, their behaviors and ways of understanding teaching, school, pedagogy and the teacher. The "naturalization" of this way of proceeding, seeing, doing and thinking constitutes a field for suspicion, exploration and investigation, insofar as it allows one to question how this discourse was constituted in a truth and under what conditions it was legitimized as a practice of pedagogical functioning. This is how the "Maestros en Colectivo" network focused on studying, from a documentary perspective, the way in which pedagogical innovation was installed as a discourse and as a practice to guide the pedagogical work of teachers, its mode of operation, its thought and its teaching, between 1980 and 2020. With this investigative exercise, an attempt is made to describe and investigate in the archive of the time, the conditions of existence that made it possible for pedagogical innovation to be installed as a truth policy in the Bogota public school, guiding the pedagogical work and their constitution as teachers. Preliminarily it was found that pedagogical innovation has been taking shape naturally in the pedagogical practices of teachers, regulating the task, without questioning its existence and consolidation. As an initial conclusion, the problematization of pedagogical innovation allows progress in the distortion of a discourse and a practice that today is configured in a regulatory device for the teacher's work.
PROPUESTA DE MODELO DE PLANEACIÓN DIDÁCTICA PARA LA ENSEÑANZA DE LA QUÍMICA A PARTIR DEL CDC
Authors:   Universidad de Baja California, Ruiz-Pino Diana del Pilar
Published: September 2023

This research aims to understand and interpret the conceptual development of didactic content knowledge (CDC) and its importance in didactic planning and the use of scientific thinking skills in teaching chemistry. The main objective is to design a didactic planning model based on CDC for chemistry teachers that facilitates the teaching of scientific thinking skills. It highlights the importance of understanding and reflecting on the CDC of teachers to strengthen the teaching process, especially scientific literacy. The research was carried out through a multiple case study with three public school teachers in the Suba locality in Bogotá. The Nvivo 11 software was used for the management of qualitative data. The results indicate that teachers have strengths in the content and articulation of psychopedagogical and contextual knowledge, but they have weaknesses in the expression and management of epistemological and historical aspects of chemical concepts. The importance of scientific thinking skills was identified as an emerging category that mobilizes other skills in the planning and teaching process. Based on the above, it is concluded that teaching chemistry is not limited to presenting content, but rather it is the CDC of teachers that mediates the process of transposition and scientific literacy. The presence of CDC categories loses relevance when there is no integration, reflection, and training in them. The recognition and use of these categories are a path to the formulation of didactic creations that integrate science with the realities of teachers and students, which will achieve a change in the habits of teaching science.
The First Journal in T-Consciousness Research
Authors: Taheri Mohammad Ali
Published: September 2023

The Journal of Cosmointel was established in 2022. It is an open-access, multidisciplinary journal that focuses on research related to T-Consciousness. The journal is published on a rolling basis to accommodate authors and allow for the flow of the large volume of written research that has been submitted and is becoming available for publication. Journal access is free to all users; registration will be available in the future to ensure receipt of updates on the publication of issues and news. The journal focuses on Sciencefact research results and is published by Cosmointel Inc.
Identification of mutations related to phytate accumulation in barley kernels
Authors: Katrii V. B. , Velikozhon L. G. , Slivka L. V. , Morgun B. V.
Published: September 2023

Aim. To conduct DNA marker testing on a sample of Ukrainian selection barley samples for identify various lpa-mutations that affect to the qualitative composition of phosphorus in barley grains. Мethods. Isolation of DNA (CTAB method), polymerase chain reaction (PCR) and electrophoretic determination of DNA amplification products in agarose gel for the identification of lpa mutations in barley grains. Results. The conducted molecular genetic studies of 20 breeding lines of hulless barley made possible to identify samples that have lpa-1 and lpa-2 mutations. The presence of such mutant genes determines a low content of organic (unavailable inorganic) phosphorus and an increased content of mineral (available) phosphorus that can be absorbed by the human body. Conclusions. The applied methods of identification of lpa-1 and lpa-2 mutations that affecting to the phytate content in whole grain barley allow for effective analysis of selection lines and mutant samples that can be used in future crossing schemes.
Selection and optimization of the method for DNA isolation and purification from Corylus species for PCR analysis
Authors: Mishchenko A. M. , Andreev I. O.
Published: September 2023

Aim.The use of molecular genetic analysis tools requires DNA preparations of high purity. However, in the case of hazelnut plants (Corylus spp.), obtaining quality DNA is complicated by the presence of a significant amount of secondary metabolites in the leaf tissue. The aim of the study was to select and optimize the method for isolation and purification of hazelnut DNA for further use in PCR analysis. Methods. Three methods of DNA isolation using cetavlon as a detergent were used, which differ in the DNA purification steps: the commonly used protocol by Doyle, Doyle, 1987; a modified method with purification using silicon dioxide microparticles; and a method using the extraction of soluble metabolites, which precedes the DNA isolation step. Results. The first two methods were shown to be unsuitable for DNA isolation from dry hazelnut leaves due to a high amount of impurities. The last method allowed to remove a significant part of water-soluble organic substances at the first stage and obtain high-quality DNA preparations. Conclusions. The method for DNA isolation from Corylus spp. plants and hybrids was selected and optimized, which allows isolation of a sufficient amount of high-quality DNA from dried leaf tissue, suitable for further molecular genetic analysis using PCR.
Algorithm for creating ILP markers for molecular genetic analysis of Triticum durum and other types of cereals
Authors: Novozhylov D. O. , Hordynskyi S. O. , Postovoitova A. S. , Rabokon A. M. , Pirko Ya. V. , Blume Y. B.
Published: September 2023

Aim. Development of an algorithm for creating ILP markers suitable for breeding studies of durum wheat (Triticum durum) and some other types of cereals. Methods. Use of classical bioinformatics tools to locate potential intron sites of target genes in T. durum suitable for use as ILP markers based on homology of T. durum contigs and rice (Oryza sativa) coding sequences. Analysis of exon-intron structure of homologous genes in O. sativa, Hordeum vulgare, Aegilops tauschii and Triricum aestivum followed by selection of appropriate primers for potential ILP markers. Results. A number of potential location sites of target introns in T. durum contig sequences were identified. Degenerate primers were designed for them, taking into account the analysis of the exon-intron structure of the corresponding homologous genes in O. sativa, H. vulgare, A. tauschii and T. aestivum. Conclusions. An algorithm for the creation of ILP markers was developed, which can be used in molecular genetic studies of plants in the case of the absence or incomplete sequenced genome of the studied species and the lack of information about the exon-intron structure of their genes. A number of potential ILP markers that can be used for cereals, in particular T. durum, have been identified.
Genetic analysis of sulfate assimilation gene cluster of Streptomyces coelicolor A3(2)
Authors: Ostash B. O. , Stanchak K. , Gren T. , Fedorenko V. O.
Published: September 2023

Aim. Streptomyces coelicolor A3(2) is the best studied species within this bacterial genus. Biosynthesis of specialized (secondary) metabolites by Streptomyces is of special interest. Primary metabolism, where all the precursors of specialized metabolites come from, is also studied in great detail. There are glaring gaps in our knowledge of sulfur metabolism in this species. We took genetic approach to probe the function of several genes within presumed sulfate assimilation gene cluster of S. coelicolor A3(2). Methods. Microbiological and genetic approaches were combined to generate mutants and to study their properties. Results. Sulfate assimilation gene cluster is structurally and functionally similar to that of phylogenetically close Corynebacterium. Most of the generated knockout strains behaved as would be expected from their molecular function inferred in silico. This confirms their involvement in sulfate uptake/conversion. Knockout of gene sco6101 (having no homologs from the other bacterial sulfate assimilation operons) impaired the growth on inorganic sulfur species and L-cysteine, pointing to its association with sulfur metabolism. Conclusions. Our study provides experimental evidence for the involvement of sco6093-sco6102 segment in sulfate assimilation, and also reveals novel gene, sco6101, essential for sulfur cycle. Further efforts are needed to elucidate the mechanism of Sco6101 action.
Calli cultures obtained via selection with heavy metal ions and their osmotic tolerance
Authors: Zaitseva I. A. , Bronnikova L. I.
Published: September 2023

Aim. It is established that the resistance to some heavy metal cations is combined with the osmotic stress tolerance. The resistance to barium ions correlates with the salinity tolerance whereas the cadmium cation resistance is combined with water stress deficit. It is known too about the differences between the tolerance levels of cell cultures and tolerance levels of plants. On this account the comparative estimation of the primary and secondary calli to simulated stresses is established. Methods. The tobacco was the object of the experiment. This plant is extremely sensitive to osmotic stresses. The salinity was simulated by the addition of sea water salts – 25.0 g/l; water stress was created by the addition of 0.8M of mannitol As calli proliferation marker relative fresh mass growth was used. Results. Primary and secondary tobacco calli there were obtained. Those cultures demonstrated resistance to lethal simulated stresses. Ва-resistant culture developed on medium with the addition of 25.0 g/l of sea water salts. Cd-resistant culture grew on medium. Conclusions. The appearance of tolerance is selected after the primary selection on media with heavy metal ions. The level of the tolerance did not decrease within the cultivating period.
Effect of nicotinic acid in defence of wheat plants against hypoxia under flooding stress
Authors: Zhuk I. V. , Shylina Ju. V.
Published: September 2023

Aim. The aim of the research was to investigate the induction of the Triticum aestivum L. non-specific tolerance by nicotinic acid to hypoxia during the flooding stress. Methods. Winter wheat plants cv. Ladyzhynka were grown in laboratory conditions and had been treated by nicotinic acid solution then flooding stress started. The hydrogen peroxide content in leaves and morphometric parameters were measured during the experimental period. Results. It is shown that the influence of the nicotinic acid was essential during the first hour of the flooding stress. After 96 hours of flooding stress the nicotinic acid balanced the hydrogenous peroxide level in wheat leaves close to control. Conclusions. Nicotinic acid decreased the stress level in wheat plants under flooding conditions via regulation of hydrogen peroxide content in leaves. The regulation of the hydrogen peroxide level could influence on plant adaptation to flooding stress and conditions of high humidity, decrease the damage of tissues and prevent their loss under flooding. Nicotinic acid could be used as a perspective component for compound combinations to induce complex stimulation of plant tolerance and defense against plant biotic and abiotic stresses.
Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene
Authors: Komisarenko A. G. , Mykhalska S. I.
Published: September 2023

Aim. To investigate the level of tolerance to water deficit of seed generations (T1 and T4) of genetically modified winter wheat with partially suppressed expression of the proline dehydrogenase gene (ProDH) based on the analysis of physiological and biochemical indicators and economic characteristics of plants. Methods. Determination of indicators of crop structure and content of free L-proline (Pro). Results. The level of Pro was studied and the main elements of productivity in the offspring of transgenic plants and their original forms under normal and insufficient water supply were analyzed. Conclusions. T1 and T4 biotechnological plants under normal/stress conditions accumulated more Pro than the original genotypes. The level of this amino acid in genetically modified seedlings under optimal cultivation conditions exceeded the initial forms by an average of 1.8 times. Under the influence of water deficit, its content increased by 2.2 and 2.3 times, in relation to normal water supply. In non-transgenic variants, the level of Pro, under similar growing conditions, was lower by 1.9 and 2.0 times, compared to T1 and T4 plants. Under the optimal water regime, the analyzed wheat variants did not differ significantly in terms of productivity. Drought led to their decrease, but the offspring of biotechnological plants were characterized by a less pronounced difference, as well as a higher yield.
Features of the expression of breeding traits in Linum usitatissimum L. somaclones
Authors: Mishchenko S. V. , Kryvosheeva L. M. , Sribnyi M. V.
Published: September 2023

Aim. Determining the level of expression, variability and features of inheritance of the main breeding traits of flax somaclones (Linum usitatissimum L.) in connection with the possibility of their use as initial selection material was the aim of our research. Methods. Hypocotyl and epicotyl segments for the induction of callusogenesis and somatic embryogenesis were cultured in vitro on the nutrient medium of Murashige and Skoog with the addition of 1.00 mg/l 6-benzylaminopurine and 0.05 mg/l 1-naphthylacetic acid, 30 g/l sucrose, a photoperiod of 16 h, illumination 2500 lux, relative air humidity 60–80 %, air temperature 22–24°C. Somaclones were adapted in vivo, and their generations were studied in the field. Results. Somaclones of the Hlinum variety were tested in the field according to the main selection traits and were not significantly inferior to the control variant; however, among them it is possible to single out some valuable individual plants for further breeding. Conclusions. Peculiarities of correlations between traits indicate the possibility of creating breeding material with a high level of fibrousness and seed productivity at the same time, high fiber mass and its content, allow selection for fibrousness by indirect traits – total and/or technical stem length, stem mass. A promising direction is the use of somaclones as sources of a high level of manifestation of the trait of the number of capsules on a plant (up to 17 pcs.).
Peculiarities of stomata morphology in bread wheat
Authors: Rymar Yu. Yu. , Pronina O. V. , Duplij V. P. , Morgun B. V.
Published: September 2023

Aim. The aim was to find and characterize different breeding lines and varieties of bread wheat according to morphological features of the stomatal apparatus for further study of the genetic patterns of stomatal biogenesis. Methods. The stomatal apparatus was evaluated by the method of impressions from the middle of the adaxial and abaxial sides of the flag leaf. Films with prints were studied with a light microscope under 100–200 total magnification. The length and width of the guard cell, stomatal pore, and number of stomata were measured, and statistical data processing was carried out. Results. An evaluation of stomata morphology was made for three varieties of bread wheat, which are characteristic of Ukraine, and three breeding lines originating from the Northern America, which differ in morphological features. The breeding line «Short-awn» had the different number of stomata on the adaxial side of the leaf, the «Dwarf» showed the increase in the total area of stomata per area unit of leaf on the adaxial side and the «Spiky, short-stem» line had a lower value of this parameter on the abaxial side and at the same time tended to decrease the total area of stomata on the abaxial side of the leaf. Conclusions. It was succeeded to find morphologically distinct lines according to the features of the stomata. The lines are valuable starting material for successive molecular genetic research of the biogenesis of wheat stomata.
Obtaining dihaploid lines of soft winter wheat with complex resistance to rust and hard smut by anther culture in vitro
Authors: Shestopal O. L. , Zambriborshch I. S. , Traskovetskaya V. A. , Vasiliev O. A. , Babayants L. T. , Chekalova M. S. , Afinogenov O. A.
Published: September 2023

Aim. Obtaining homozygous linear material of winter soft wheat of different genetic origin (complex hybrids), which are resistant to different types of rust by androgenesis in vitro. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Methods of phytopathological assessment of resistance to complex of various diseases. Results. The differences in the frequency of сallus induction (from 0.99 to 16.26 % of planted anthers) and the ability to regenerate plants (from 0 to 3.05 % of planted anthers) in the process of androgenesiss in vitro of winter soft wheat were detected. Fertile regenerants in the anthers culture 5 out of 9 studied hybrids have been obtained. The effectiveness of the last process was different: from 12.5 to 60.0 % (an average of 29.41 %) from the received regenerants. The high degree (8–9 points) of the resistance of the obtained dihaploids to a complex of diseases was shown. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. The highest level of callus formation were shown for samples 120/20 and 132/20. 15 dihaploid lines with complex rust resistance and hard smut were obtained.
Influence of dextran-70 on the cytotoxic effect of EMAP II on glioma cells in vitro
Authors: Shuba I. M. , Lylo V. V. , Karpova I. S. , Glavatskyi O. Y. , Kornelyuk O. I.
Published: September 2023

Aim. To evaluate with the use of the MTT test how the formation of a nanocomposite complex of the cytokine EMAP II with dextran-70 affects the cytotoxic effect of the free protein in glioma cell cultures of different origins: a standart culture U251MG cells and a primary culture of cells obtained from tumor fragments. Methods. The recombinant polypeptide EMAP II was obtained using gene engineering biotechnology. The study was conducted on the standard human glioma cell line U251MG and on primary cell cultures obtained from tumor fragments after surgical removal. Cell viability was determined using the MTT test. The cells were cultivated for a day with t the different concentrations from 1.0 pM to 10 μM of EMAP II and the EMAP II + dextran-70 complex im serum-free standard DMEM growth medium. Results. In our previous works, it has been shown that EMAP II exhibited dose-dependent cytotoxic properties in the concentration range of 1.0 pM – 10 μM on U251MG glioma cells and in primary cell cultures. The dose-effect curve in all cases has a complex pattern. Dextran-70 does not fundamentally affect the cytotoxic effect of EMAP II, but depending on the dose and type of cells, it shows the ability to slightly weaken/enhance the effect of the free protein. An increased sensitivity of the primary culture of glioma cells to the cytotoxic effect of the EMAP II + dextran-70 complex was revealed. Conclusions. Dextran-70 does not fundamentally affect the cytotoxic effect of EMAP II, but depending on the dose and type of cells, it shows the ability to slightly weaken/enhance the effect of the latter.
Mexican argemone (Argemone mexicana L.) CYP719A14 protein bioinformatic analysis
Authors: Slishchuk H. , Meshcheryakov V. I. , Volkova N. E. , Zakharova O. A.
Published: September 2023

CYP719A14 structure analysis and its interaction with ligands via bioinformatic methods. Methods. Homologous modeling of three-dimensional structure of enzyme. Molecular docking. Results. CYP719A14 enzyme, which shows cheilanthifoline synthase activity, model was build based on results of homologous modeling. Interaction of built model with possible substrates – benzylisoquinoline compounds: (S)-Scoulerine, (S)-Cheilanthifoline, (S)-Tetrahydrocolumbamine, (S)-Reticuline was investigated using molecular docking method, thermodynamic characteristics of interaction were calculated. Potential Ligand Access Chanel – potential active center was identified. Conclusions. Potential Ligand Access Chanel CYP719A14 enzyme, which is formed by aminoacids residues PHE57-GLY69, ALA285-ARG310, THR369-HSD390 researched, allowing to use discovered data in alkaloid engineering research with aim to improve Mexican argemone alkaloid profile.
Evaluation of collection samples of soft winter wheat of the Ivanivsk research and selection station as starting material for hybridization
Authors: Torianyk V. M.
Published: September 2023

Aim. Highlighting the results of the study of breeding value in terms of winter hardiness, plant height, weight of 1000 seeds and yield variety samples of soft winter wheat cultivars of domestic selection, selected for hybridization in the collection nursery of the Institute's Ivanivka research and breeding station of bioenergy crops and sugar beets of the National Agricultural Academy of Ukraine. Methods. The research was conducted in sowing in the 2020-2021 cropping year in the southern forest-steppe zone of Ukraine. The object of the study were variety samples of the domestic selection of the lutescens type (10) and erythrospermum (15), who grew and studied their biological and economic characteristics according to generally accepted classical methods. Results. The average value of winter hardiness of the studied variety samples of both types was above 8 points. All studied variety samples were medium-sized with a low level of plant height variability. The weight of 1,000 seeds of the studied variety samples was about 44 g on average, the level of variability for this characteristic was low in the lutescens type, and medium in the erythrospermum type. The average yield of variety samples of both types was higher than 6 t/ha. Conclusions. The studied variety samples were characterized by an optimal and high level of manifestation of economic and valuable traits, therefore they were recommended for practical use in breeding as a starting material for hybridization. The studied variety samples are suitable for use in production crops in the Southern Forest-Steppe zone of Ukraine.
Study of trinucleotide CAG-repeats expansion in androgen receptor gene among patients with suspected Kennedy’s syndrome
Authors: Tretiak B. I. , Tyrkus M. Ya. , Bakum Kh. Ya. , Zastavna D. V. , Akopyan H. R.
Published: September 2023

Aim. To perform a molecular genetic study of CAG-repeat expansion in androgen receptor gene AR in individuals with suspected spinal and bulbar muscular atrophy (Kennedy’s syndrome). Methods. Clinical and genealogical, method of differential diagnosis, DNA isolation and purification, molecular genetic: polymerase chain reaction, electrophoresis in agarose gel. Results. A molecular genetic study of trinucleotide CAG-repeats expansion in androgen receptor gene in 30 people with suspected Kennedy’s syndrome was performed. In 5 probands of the study group, 38 CAG repeats (the upper limit of the norm) were established and in 27 examined patients, the number of CAG repeats did not exceed 37 (the norm). Among the examined group of patients, was found a family in which three men had 49 CAG repeats in the AR gene, which confirmed the presence of Kennedy’s syndrome. Conclusions. Kennedy’s syndrome is a rare X-linked recessive disease that requires the development of specific biomarkers to clarify the pathogenic process and facilitate early diagnosis.
Population genetic analysis of the population of Luhansk oblast
Authors: Shuba V. V. , Gorpynchenko М. Yu. , Atramentova L. O.
Published: September 2023

Aim. The aim was to analyze the population of Luhansk region and to investigate the possibility of surnames to differentiate populations at the district level. Methods. The general population census database of Luhansk oblast was used. Calculations of indicators of the population structure of the regional and eighteen district populations were carried out according to I. Barrai. Results. The structure of the Luhansk regional population is characterized by the isonymy index І (2.4×10-4), the FST inbreeding coefficient (6.0×10-5), the migration index v (18.7×10-4), the diversity index a (41.7×102), entropy H (14.6) and surname redundancy R (31.0). Indicators of district populations vary widely: I from 3.5×10-4 in the industrial areas of the south of the region to 23.7×10-4 in the north. The FST is in the range 8.7–59.3×10-5, v — 69.3–241.9×10-4, a — 4.3–29.0×102, H — 9.7–13.4, R — 24.1–30.2. Conclusions. The results obtained with the surnames testify to the significant impact of human economic activity on the population structure, which is reflected in isonymy, inbreeding and migration.
Peculiarities of the protein complex of seeds of pea (Pisum sativum L.)
Authors: Molodchenkova O. O. , Kartuzova T. V. , Lavrova G. D. , Koblay S. V. , Mishchenko L. T. , Levitsky Yu. A. , Bezkrovna L. Ya.
Published: September 2023

Aim. To investigate the features of the protein complex of pea seeds (Pisum sativum L.) of varieties of Ukrainian and foreign breeding of different morphotypes for use at the selection of varieties of food direction. Methods. Standard and developed in Laboratory of Plant Biochemistry methods of plant biochemical analysis (Kjeldahl method, spectrophotometric methods, electrophoresis). Statistical analysis of research results was carried out using the programs Libre Office Calc (GNU Lesser General Public Licensev3), “Imagel”. Results. The study of the content of protein, 11S and 7S globulins, their ratio in the seeds of peas of Ukrainian and foreign varieties showed the presence of reliable varietal differences according to the studied characteristics depending on their morphotype. Using electrophoretic and densitometric analyses, varietal differences in the intensity of bands, the presence or absence of some components in the electrophoretic spectra of 11S and 7S globulins, which affect the nutritional value of pea seeds, were established. Conclusions. The obtain results shown that it will be possible to select genotypes of food direction using the studied biochemical characteristics of evaluation.
Cytogenetic changes in irradiated rats under influence of guaninium-zinc chloride complex
Authors: Shamilov E. N. , Abdullayev A. S. , Shamilli V. E. , Farajov M. F. , Azizov I. V. , Hajiyev K. A. , Jalilova A. A.
Published: September 2023

Aim. The main goal of this study was to study the anti-radiation effect of guaninium-zinc chloride complex on cytogenetic changes in irradiated rats. Methods. White rats of the Wistar line with an average weight of 110-125 grams served as the material for the detection of the anti-radiation effect of guaninium-zinc chloride complex. Animals were injected intraperitoneally with solutions of guaninium-zinc chloride complex at a concentration of 40 mg/kg 2 hours before irradiation and were subjected to general uniform gamma irradiation from a 60Co source in the Rukhund-20000 irradiation unit at a dose of 3 and 5 Gy. Results. Cytogenetic analysis of animals showed that with a single injection of guaninium-zinc chloride complex solutions before irradiation, the proportion of chromosomal abnormalities decreased markedly, and reached 2.93 %, and the amount of abnormal spermatozoa markedly decreased. Guaninium-zinc chloride complex softened the damaging effects of ionizing radiation. There was no suppression of cell division. Conclusions. It was found for the first time that, with the introduction of a solution of guaninium-zinc chloride complex in white Wistar rats before irradiation, it reduces the damaging effect of gamma irradiation. The obtained data gives grounds to assume that the guaninium-zinc chloride complex tested by us can be recommended as a promising anti-radiation agent.
Walther Flemming: life path and scientific legacy
Authors: Mayorova O. Yu. , Prokopiak M. Z. , Mishchuk N. Y. , Zagrychuk O. M. , Hrytsak L. R. , Drobyk N. M.
Published: September 2023

The main stages of Walther Flemming life and activity are considered in the article. Walther Flemming (21.04.1843–4.08.1905) is German biologist, anatomist, one of the most famous histologists of the 19th century and the founder of cytogenetics. He studied medicine at four famous universities of the German Empire – in Göttingen, Tübingen, Berlin and Rostock. After defending his thesis, in 1868 he started teaching anatomy and histology at Würzburg, Amsterdam and Rostock universities, Germany and Charles Ferdinand University, the Czech Republic. During the last 25 years of his life, he was a professor of anatomy at the Christian Albrecht University in Kiel, Germany and headed one of its departments – the anatomical institute.

At the beginning of W. Flemming scientific activity, his research mostly concerned histology and anatomy, as well as the development of histological methods and fixation of microscopic specimens. However, the main works of the scientist are devoted to study cell division process and the chromosomes distribution in daughter nuclei. He firstly introduced the concept of “mitosis”, which he studied on stained microscopic preparations, using salamander epithelial cells as a source of biological material. W. Flemming described the processes in the nucleus, distinguished “progressive” and “regressive” phases of cell division. He demonstrated that the cells contain areas that absorb basophilic dyes. These areas were later called chromatin; other structures remained unstained under such conditions and were therefore called achromatin. W. Flemming studied the structure and functions of the spindle during cell division, and he also studied the polar structure, which he called centriole. The scientist found that indirect division usually occurs not only in vertebrates, but also in invertebrates and protists. He established that two successive, morphologically distinct divisions of nuclear occur during spermatogenesis, resulting in the division of chromatin into two daughter cells. Thus, W. Flemming’s research contributed to the development of cell biology and its branches.
Omkostninger ved hjernesygdomme i Danmark
Authors: Olesen Jes , Andlin-Sobocki Patrik , Sestoft Dorte M. , Truelsen Thomas , Jönsson Bengt
Published: September 2023

Formålet med undersøgelsen var at estimere antallet af personer med hjernesygdomme samt de associerede direkte og indirekte udgifter. Arbejdet var del af et pan-europæisk projekt initieret af det Europæiske Hjerne Råd, European Brain Council, hvor medlemmerne er Europæiske organisationer indenfor psykiatri, neurologi, og neurokirurgi, basal neuroforskning, samt europæiske patientorganisationer. Projektet har det formål, at tilvejebringe de bedst mulige estimater for de økonomiske omkostninger ved hjernesygdommene i Europa baseret på den forhåndenværende internationale videnskabelige litteratur. Formålet med nærværende publikation er at præsentere resultater for Danmark og diskutere dem i relation til den nationale videnskabelige litteratur.
A phenomenological exploration of technology start-up failure in Sri Lanka
Authors:   MBA, MSc in Advanced Software Engineering, School of Computer Science and Engineering, University of Westminster, London W1B 2HW, United Kingdom, e-mail: w1851993@my.westminster.ac.uk, Wimal Kesara , Ajendra Selvaratnam ,   MBA, ACMA, CGMA, Lecturer, Global Banking School (GBS), Greenford UB6 0HE, United Kingdom, e-mail: growingajay@gmail.com
Published: September 2023

PURPOSE: The main purpose of this qualitative study was to explore tech start-up failures in Sri Lanka to emerge themes that explain the critical factors that are impacting failures of Sri Lankan tech start-ups and also to identify recommendations that could help evade those factors. The paper also presents the finding to enrich tech entrepreneurs to build their strategies with an understanding of factors that leads to failure and to make well-educated decisions. METHODOLOGY: The study is based on a qualitative research approach that helps to present findings in a theoretical way. A phenomenological analysis has been used to identify, understand, and analyze the phenomena of tech start-up failures. Twelve start-up leaders participated in this study and shared their lived experiences of tech start-up failures in Sri Lanka. Interviews were conducted with them based on twelve interview questions and twelve core themes emerged based on the participants’ lived experiences. In analyzing data, the modified Van Kaam approach was used, utilizing a seven-step framework that considers the structural and textual aspects of experiences, as well as the perceptual characteristics of the phenomenon. FINDINGS: The themes answered the key research question of the study: What are the critical factors that are impacting on failures of tech start-ups in Sri Lanka? The cause of tech start-up failures according to the current study varied including, financial uncertainty, no market research, no product–market fit, paranoid behaviors of innovators, lack of timely response to changing conditions, and location of the venture. IMPLICATIONS: The paper concisely presents twelve critical reasons for tech start-up failures. The results of the research will enable Sri Lankan tech start-ups to identify key factors of failure for the growth of their surviving strategies. Identifying secret obstacles in the industry helps entrepreneurs prepare for pitfalls and provides guidelines for policymakers to make informed choices when implementing national policies. More importantly, it has been discovered that the major areas that require more attention are leadership, funding, marketing, and innovation. Finally, four groups of recommendations have been discussed under financing, market research, leadership, and inventors. ORIGINALITY AND VALUE: The comparison of the current study themes with the findings of related studies is inconclusive because the literature on tech start-up failures in other countries and in Sri Lanka is minimal. Some of the themes align with the findings of research conducted in other countries, although there were some themes that were explored uniquely.