bluuebunny/crossref_metadata_2025 huggingface dataset:
N6-benzyladenine (BAP)-Based Seed Preconditioning Enhances the Shoot Regeneration of Seedling-Derived Explants for Subsequent Indirect Gene Transfer in Soybeans (Glycine max [L.] Merrill.)
Authors: Sehaole Esmerald Michel Khomotso , Mangena Phetole
Published: April 2024

This study evaluated the effects of N6-benzyladenine (BAP) seed preconditioning and seedling-derived explants on in vitro plant regeneration potential in soybeans (Glycine max [L.] Merrill.). The findings showed that seed preconditioning with 2.55 mg/L BAP prior to germination significantly influenced seedling establishment and the development of shoots, shoot elongation, and rooting on MS media supplemented with BAP and TDZ, compared to the negative (MS-NC) and positive (MS-NP) controls. The results also showed significant differences based on the genotypes, with Dundee recording 91.0% germination over a minimum of 5 days, compared to 74.2% with Peking, followed by 87.5% and 80.0% overall shoot induction frequency in these genotypes, respectively. Regenerated shoots were successfully elongated on MS medium supplemented with 0.5 mg/L BAP plus 0.6 mg/L GA3 and rooted on hormone-free medium, for 3‒4 weeks, and then hardened in the acclimatization growth room under elevated light levels. Overall, this study revealed that BAP preconditioning of seeds enhances the frequency of bud initiation and shoot proliferation, mostly in whole-seedling and cotyledonary node explants subcultured on MS-E and MS-A media supplemented with BAP in combination with TDZ.
The Genetic Homogeneity of Uganda’s East African Highland Bananas (Mutika/Lujugira) Does Not Match the Extensive Morphological Variation Identified in this Subgroup
Authors: Pillay Michael
Published: April 2024

The East African Highland banana (Mutika/Lujugira subgroup) is composed of triploid (AAA) cooking and beer banana varieties that are adapted to the high-altitude region of the Great Lakes region of East Africa. Banana production is affected by several biotic and abiotic factors. Breeding opportunities in bananas are limited due to female sterility and parthenocarpy. The genetic diversity of crops enables breeders to develop new germplasm. Molecular markers have been used widely to dissect crop plants’ genetic diversity. This study assessed the genetic variation in 27 varieties from the Mutika/Lujugira subgroup using random amplified polymorphic DNA (RAPD). No genetic variation was observed among the banana varieties, and the 18 ten-mer primers produced monomorphic banding profiles. The genetic homogeneity of this banana subgroup is not congruent with their extensive morphological variation. Domestication and the bottleneck effect are often cited as the cause of reduced diversity in crop plants. On the other hand, several mechanisms, including somatic mutations, transposable elements, polyploidy, genome plasticity, and epigenetic mechanisms, are known to increase plant phenotypic variability. Further in-depth research is needed to explain the puzzle between the genetic and morphological diversity in the Mutika/Lujugira subgroup.
‘Charge Reverse’ Halogen Bonding Contacts in Metal-Organic Multi-Component Compounds: Antiproliferative Evaluation and Theoretical Studies
Authors: Banik Subham , Baishya Trishnajyoti , Gomila Rosa M. , Frontera Antonio , Barcelo-Oliver Miquel , Verma Akalesh K. , Das Jumi , Bhattacharyya Manjit K.
Published: April 2024

Two new metal–organic multi-component compounds of Ni(II) and Co(II), viz. [Ni(3-CNpy)2(H2O)4]ADS·2.75H2O (1) and [Co(3-CNpy)2(H2O)4](4-ClbzSO3)2 (2) (3-CNpy = 3-cyanopyridine, ADS = anthraquinone-1,5-disulfonate, 4-ClbzSO3 = 4-chlorobenzenesulfonate), were synthesized and characterized using single crystal XRD, TGA, spectroscopic (IR, electronic) and elemental analyses. Both the compounds crystallize as multi-component compounds of Ni(II) and Co(II), with uncoordinated ADS and 4-ClbzSO3 moieties in the crystal lattice, respectively. Crystal structure analyses revealed the presence of antiparallel nitrile···nitrile and π-stacked assemblies involving alternate coordinated 3-CNpy and uncoordinated ADS and 4-ClbzSO3 moieties. Moreover, unconventional charge reverse Cl∙∙∙N halogen bonding contacts observed in compound 2 provide additional reinforcement to the crystal structure. Theoretical calculations confirm that the H-bonding interactions, along with anion–π(arene) and anion–π(CN) in 1 and π–π, antiparallel CN···CN and charge reverse Cl···N halogen bonds in 2, play crucial roles in the solid state stability of the compounds. In vitro anticancer activities observed through the trypan blue cell cytotoxicity assay reveal that the compounds induce significant concentration dependent cytotoxicity in Dalton’s lymphoma (DL) cancer cells, with nominal effects in normal healthy cells. Molecular docking studies reveal that the compounds can effectively bind with the active sites of anti-apoptotic proteins, which are actively involved in cancer progression.
Resistance to Cassava Whitefly (Bemisia tabaci) among Eastern and Southern African Elite Cassava Genotypes
Authors: Sam Wamani , Morris Opio Samuel , Tom Omara , Patrick Ocitti , Colvin John , Abu Omongo Christopher
Published: April 2024

Cassava whitefly, Bemisia tabaci, directly damages cassava leaves by feeding on phloem, causing chlorosis and abscission, leading to a yield loss of up to 50%. The pest also causes indirect damage through sooty mold formation. Most Ugandan cassava varieties resist cassava mosaic disease (CMD) and tolerate cassava brown streak disease (CBSD), but little is known about their response to whitefly infestation. The main objective of this study was to identify cassava genotypes with putative resistance to whitefly in Uganda. This was conducted on 24 improved cassava varieties in three agro-ecological zones during the second rains of 2016. Monthly data were taken for adult and nymph counts, whitefly and sooty mold damage, and CMD and CBSD severities from 2 to 9 months after planting (MAPs). The results show that the whitefly population is highly significantly (p < 0.000) amongst varieties across the three agro-ecological zones. Mkumba consistently supported the low adult numbers and nymphs. The findings demonstrate the potential of the improved cassava varieties as sources of whitefly resistance for sustainable management.
Risk of Cardiac Implantable Electronic Device Infection after Early versus Delayed Lead Repositioning
Authors: Schvartz Noemi , Haidary Arian , Wakili Reza , Hecker Florian , Kupusovic Jana , Zsigmond Elod-Janos , Miklos Marton , Saghy Laszlo , Szili-Torok Tamas , Erath Julia W. , Vamos Mate
Published: April 2024

(1) Background: Early reintervention increases the risk of infection of cardiac implantable electronic devices (CIEDs). Some operators therefore delay lead repositioning in the case of dislocation by weeks; however, there is no evidence to support this practice. The aim of our study was to evaluate the impact of the timing of reoperation on infection risk. (2) Methods: The data from consecutive patients undergoing lead repositioning in two European referral centers were retrospectively analyzed. The odds ratio (OR) of CIED infection in the first year was compared among patients undergoing early (≤1 week) vs. delayed (>1 week to 1 year) reoperation. (3) Results: Out of 249 patients requiring CIED reintervention, 85 patients (34%) underwent an early (median 2 days) and 164 (66%) underwent a delayed lead revision (median 53 days). A total of nine (3.6%) wound/device infections were identified. The risk of infection was numerically lower in the early (1.2%) vs. delayed (4.9%) intervention group yielding no statistically significant difference, even after adjustment for typical risk factors for CIED infection (adjusted OR = 0.264, 95% CI 0.032–2.179, p = 0.216). System explantation/extraction was necessary in seven cases, all being revised in the delayed group. (4) Conclusions: In this bicentric, international study, delayed lead repositioning did not reduce the risk of CIED infection.
Impaired Carbohydrate Metabolism among Women with Chronic Low Back Pain and the Role of Dietary Carbohydrates: A Randomized Controlled Cross-Over Experiment
Authors: Elma Ömer , Tümkaya Yılmaz Sevilay , Nijs Jo , Clarys Peter , Coppieters Iris , Mertens Evelien , Malfliet Anneleen , Deliens Tom
Published: April 2024

Background: Impaired glucose regulation is suggested to be related to chronic low back pain (CLBP), although it is not clear how they interact with each other. Thus, the primary aim of this study was to investigate differences in postprandial glycemic responses (PPGRs) (the first sign of impaired glucose metabolism) to high- (sucrose) and low-glycemic index (GI) (isomaltulose) beverages in normoglycemic women with CLBP and healthy controls (HCs) and explore whether any group that showed greater PPGRs to high-GI beverage intake would benefit when the high-GI beverage was replaced with a low-GI beverage. Secondly, this study aimed to explore the association between PPGR and pain in patients with CLBP. Methods: This study was registered at clinicaltrials.org (NCT04459104) before the start of the study. In this study, 53 CLBP patients and 53 HCs were recruited. After 11–12 h of fasting, each participant randomly received isomaltulose or sucrose. Blood glucose levels were measured during the fasting state and 15, 30, 45, 60, 90, and 120 min after the beverage intake, and each participant underwent experimental pain measures. Results: Compared to the HCs, the CLBP group showed significantly higher PPGRs to sucrose (p < 0.021). Additionally, the CLBP group showed a significantly higher decrease in PPGR (p = 0.045) when comparing PPGR to sucrose with PPGR to isomaltulose. Correlation analysis revealed a positive association between self-reported pain sensitivity and PPGR to sucrose, while there was no association found between any experimental pain measures and glycemic responses. Conclusions: Overall, these findings suggest that normoglycemic CLBP patients might have a higher risk of developing impaired glucose tolerance than the HCs and might benefit more when high-GI foods are replaced with low-GI ones.
“Ablate and Pace” with Conduction System Pacing: Concomitant versus Delayed Atrioventricular Junction Ablation
Authors: Palmisano Pietro , Ziacchi Matteo , Dell’Era Gabriele , Donateo Paolo , Bartoli Lorenzo , Patti Giuseppe , Senes Jacopo , Parlavecchio Antonio , Biffi Mauro , Accogli Michele , Coluccia Giovanni
Published: April 2024

Objectives: Conduction system pacing (CSP) and atrioventricular junction ablation (AVJA) improve the outcomes in patients with symptomatic, refractory atrial fibrillation (AF). In this setting, AVJA can be performed simultaneously with implantation or in a second procedure a few weeks after implantation. Comparison data on these two alternative strategies are lacking. Methods: A prospective, multicentre, observational study enrolled consecutive patients with symptomatic, refractory AF undergoing CSP and AVJA performed in a single procedure or in two separate procedures. Data on the long-term outcomes and healthcare resource utilization were prospectively collected. Results: A total of 147 patients were enrolled: for 105 patients, CSP implantation and AVJA were performed simultaneously (concomitant AVJA); in 42, AVJA was performed in a second procedure, with a mean of 28.8 ± 19.3 days from implantation (delayed AVJA). After a mean follow-up of 12 months, the rate of procedure-related complications was similar in both groups (3.8% vs. 2.4%; p = 0.666). Concomitant AVJA was associated with a lower number of procedure-related hospitalizations per patient (1.0 ± 0.1 vs. 2.0 ± 0.3; p < 0.001) and with a lower number of hospital treatment days per patient (4.7 ± 1.8 vs. 7.4 ± 1.9; p < 0.001). Conclusions: Concomitant AVJA resulted as being as safe as delayed AVJA and was associated with a lower utilization of healthcare resources.
The Expression and Amplification of HER2 Has a Significant Impact on the Prognosis of Endometrial Carcinoma in Korean Patients
Authors: Kim Wook Youn , Yang Eun Jung , Jang Eun Bi , Lee A Jin , So Kyeong A , Shim Seung-Hyuk , Kim Tae Jin , Lee Sun Joo
Published: April 2024

Objective: The purpose of this study was to analyze the protein overexpression and gene amplification of HER2 in endometrial carcinoma (EC) and to evaluate its role as a prognostic factor in Korean women. Methods: A tissue microarray (TMA) was constructed from samples from 191 patients with diverse histologic types of EC. HER2 protein expression and gene amplification status were analyzed using immunohistochemistry (IHC) and silver in situ hybridization (SISH), respectively. All patients were treated and followed up at a single tertiary medical center in Seoul, Korea, between July 2009 and October 2020. Results: In terms of histological type, among the 191 EC patients, 157 had endometrioid carcinoma, nine had uterine serous papillary carcinoma (USPC), one had clear cell carcinoma, one had squamous cell carcinoma, eight had mixed carcinoma, and 15 had uterine carcinosarcoma (UC). HER2 protein overexpression was observed in eight of the 191 (4.2%) EC patients; of these patients, five had IHC scores of 2+, and three had IHC scores of 3+. The HER2 overexpression rates of USPC, UC, and endometrioid carcinomas were 33.3%, 26.6%, and 0.6%, respectively. HER2 protein overexpression was significant in USPC and UC tissues (p < 0.000) and was associated with poor overall survival (OS) (p < 0.001). HER2 gene amplification was confirmed in seven of 184 patients (3.8%), including three patients with USPC and four patients with UC. OS was significantly shorter in patients who had HER2 amplification (p < 0.001). On multivariate analysis, HER2 expression and HER2 amplification were statistically significantly associated with worse OS (p = 0.006). However, HER2 expression without amplification was not statistically associated with OS (p = 0.993). Conclusions: HER2 protein overexpression and gene amplification are significantly correlated with shorter OS in Korean women. HER2 can be considered an important predictor of survival outcomes in EC patients.
An Evaluation of the Fracture Resistance of Teeth with Simulated External Cervical Resorption Cavities Categorized Using Three-Dimensional Classification
Authors: Şahin Mantı Arzu , Ulusoy Özgür İlke
Published: April 2024

(1) Background: External cervical resorption causes dental hard tissue destruction that may reduce the fracture resistance of affected teeth. By using a compressive strength test, this study aimed to evaluate the fracture resistance of teeth with simulated external cervical resorption cavities that have different three-dimensional classifications. (2) Methods: In total, 170 teeth with simulated external cervical resorptions were divided into 16 experimental groups (n = 10) and 1 control group (n = 10) based on the three-dimensional classification: 1Ap, 1Bp, 1Cp, 1Dp, 2Ap, 2Bp, 2Cp, 2Dp, 3Ap, 3Bp, 3Cp, 3Dp, 4Ap, 4Bp, 4Cp, 4Dp and a control group. Defects were restored with mineral trioxide aggregate. The fracture resistances of the samples were statistically analyzed using two-way repeated ANOVA and the Bonferroni correction for multiple comparisons at a significance level of p < 0.05. (3) Results: The lowest resistance to fracture was observed in samples with vertical height level “4” and circumferential spread of “D” (p < 0.001). In the groups with circumferential spreads “B”, “C” and “D”, there were significant differences between the samples with vertical height levels “1”, “2”, “3” and “4” regarding fracture resistance (p < 0.001). (4) Conclusions: The circumferential spread and vertical height of the external cervical resorption influenced the fracture resistance of the affected teeth.
Concept and Diagnostic Challenges of Renal-Limited Hemophagocytic Syndrome/Macrophage Activation Syndrome
Authors: Uchida Takahiro , Oda Takashi
Published: April 2024

Hemophagocytic syndrome/macrophage activation syndrome (HPS/MAS) is a serious clinical condition that frequently leads to multiple organ failure, including acute kidney injury (AKI). Although the pathogenesis of AKI is not yet fully understood, it is believed to result from uncontrolled activation of the immune system involving macrophages and cytotoxic lymphocytes. Renal histology in HPS/MAS often presents with characteristic foamy glomerular lesions (glomerular lipidosis) with massive macrophage infiltration, known as histiocytic glomerulopathy. In this review, we introduce the recently proposed concept of renal-limited HPS/MAS as a novel etiology of histiocytic glomerular lipidosis. Patients with renal-limited HPS/MAS often develop AKI but do not fulfill the diagnostic criteria for HPS/MAS because their systemic manifestations are less severe. Therefore, the diagnosis largely depends on characteristic histological findings, that is, diffuse and global glomerular accumulation of foamy macrophages and cytotoxic lymphocytes accompanied by the interaction of these cells as well as the exclusion of various differential diseases. Although there are no established therapeutic regimens, these patients receive various types of therapies, including high-dose glucocorticoids, immunosuppressants, or anti-interleukin-1 drug, and generally achieve favorable outcomes. We summarized the concept, diagnostic challenges, and recent topics of this disease entity and discussed treatment options based on our own experiences.
Effect of Different Induction Immunosuppression on the Incidence of Infectious Complications after Kidney Transplantation—Single Center Study
Authors: Vnučák Matej , Graňák Karol , Beliančinová Monika , Kleinová Patrícia , Blichová Tímea , Doboš Vladimír , Dedinská Ivana
Published: April 2024

Background/Objectives: Potent immunosuppression lowers the incidence of acute graft rejection but increases the risk of infections. In order to decrease either infectious complications or acute rejection, it is necessary to identify risk groups of patients profiting from personalized induction immunosuppressive treatment. The aim of our analysis was to find whether there were higher incidences of infectious complications after kidney transplantation (KT) in groups with different induction immunosuppressive treatment and also to find independent risk factors for recurrent infections. Materials: We retrospectively evaluated all patients with induction treatment with basiliximab after kidney transplantation from 2014 to 2019 at our center relative to age- and sex-matched controls of patients with thymoglobulin induction immunosuppression. Results: Our study consisted of two groups: basiliximab (39) and thymoglobulin (39). In the thymoglobulin group we observed an increased incidence of recurrent infection in every observed interval; however, acute rejection was seen more often in the basiliximab group. A history of respiratory diseases and thrombocytopenia were identified as independent risk factors for recurrent bacterial infections from the first to sixth month after KT. Decreased eGFR from the first month, infections caused by multi-drug-resistant bacteria, and severe infections (reflected by the need for hospitalization) were identified as independent risk factors for recurrent bacterial infections from the first to the twelfth month after KT. Conclusions: We found that in the group of patients with thymoglobulin induction immunosuppressive treatment, infectious complications occurred significantly more often during the entire monitored period with decreased incidence of acute humoral and cellular rejection occurred more often.
Are the Spinal Changes in the Course of Scoliogeny Primary but Secondary?
Authors: Grivas Theodoros B. , Vasiliadis Elias , Mazioti Christina , Papagianni Despina , Mamzeri Aristea , Chandrinos Michail , Vynichakis George , Athanasopoulos Konstantinos , Christodoulides Paschalis , Jevtic Nikola , Pjanic Samra , Ljubojevic Danka , Savvidou Olga , Kaspiris Angelos , Grunstein Jarrett
Published: April 2024

In this opinion article, there is an analysis and discussion regarding the effects of growth on the spinal and rib cage deformities, the role of the rib cage in scoliogeny, the lateral spinal profile in adolescent idiopathic scoliosis (AIS), the genetics and epigenetics of AIS, and the interesting and novel field investigating the sleep impact at nighttime on AIS in relation to the sequence of the scoliogenetic changes in scoliotics. The expressed opinions are mainly based on the published peer-reviewed research of the author and his team of co-authors. Based on the analysis noted above, it can be postulated that the vertebral growth changes in the spine during initial idiopathic scoliosis (IS) development are not primary-intrinsic but secondary changes. The primary cause starting the deformity is not located within the vertebral bodies. Instead, the deformations seen in the vertebral bodies are the secondary effects of asymmetrical loads exerted upon them, due to muscular loads, growth, and gravity.
The Factors Influencing Chronic Kidney Disease Incidence: Database from the Korean National Health Insurance Sharing Service (NHISS)
Authors: Ko Ho-Joon , Ahn Soon-Ki , Han Suyeon , Kim Moo-Jun , Na Ki Ryang , Park Hyerim , Choi Dae Eun
Published: April 2024

Background: The global prevalence of chronic kidney disease (CKD) is increasing, with diabetes accounting for the highest proportion. We analyzed the influence of clinical factors on the incidence of CKD according to the renal function, primary focusing on patients with diabetes. Methods: We used the Sample Cohorts Database provided by the National Health Insurance Sharing Service (NHISS) in Korea. Participants aged ≥ 40 years who underwent a health checkup in 2009 were categorized into six groups based on their eGFR values (<60 mL/min, 60–89 mL/min, ≥90 mL/min) and the presence of diabetes. And all patients with CKD at 2009 screening were excluded. The participants were tracked from 2010 to 31 December 2019. The CKD incidence rate according to the eGFR values and the effect of the accompanying factors on CKD incidence were confirmed. Results: 148,089 people without CKD were analyzed. The CKD incidence rate was highest in those with eGFR < 60 mL/min with diabetes and lowest in those with eGFR ≥ 90 mL/min without diabetes. The CKD incidence rates were similar between the eGFR < 60 mL/min group without diabetes and the eGFR 60–89 mL/min group with diabetes. Compared to under 44 years of age, the hazard ratio of CKD incidence was 8 times higher in over 75 years of age. Men had a 1.7-fold higher risk of developing CKD than women. Current smoker, hypertension, dyslipidemia, myocardial infarction history, and atrial fibrillation and flutter increased the risk of CKD incidence. Age, diabetes, and baseline eGFR are important factors in the occurrence of CKD. As age increases, the risk of developing CKD in men increases compared to women. Conclusions: These results will be helpful in predicting risk groups for CKD and establishing strategies to lowering CKD incidence.
The Impact of Technical Innovations and Donor-Site Mesh Repair on Autologous Abdominal-Based Breast Reconstruction—A Retrospective Analysis
Authors: Promny Theresa , Huberth Paula , Müller-Seubert Wibke , Promny Dominik , Cai Aijia , Horch Raymund E. , Arkudas Andreas
Published: April 2024

Background: The aim of this study was to examine the potential benefit that may be achieved through the introduction of technical innovations and the incorporation of mesh for fascial donor site closure in uni- and bilateral autologous breast reconstruction with abdominal tissue. Methods: A retrospective single-center review of all breast reconstructions with a DIEP or MS-TRAM flap between January 2004 and December 2019 was performed. Donor and recipient site complications and operation times were evaluated before and after the implementation of coupler anastomoses, preoperative computed tomography angiography (CTA), indocyanine green (ICG) angiography, and the inclusion of mesh in donor site repair. Results: A total of 396 patients were included, accounting for 447 flaps. Operation time was significantly shorter in unilateral reconstructions after the implementation of CTA (p < 0.0001). ICG angiography significantly reduced the rates of partial flap loss (p = 0.02) and wound healing disorders (p = 0.02). For unilateral reconstructions, abdominal bulging or hernia was observed more often in MS1-TRAM flaps without synthetic mesh repair (p = 0.001), whereas conservatively treated seroma developed more frequently after mesh implantation (p = 0.03). Conclusions: Recent technological advancements developed over the past few decades have made a substantial impact on decreasing surgical duration and enhancing procedure safety.
Nationwide Screening Practices for Tamoxifen Retinal Toxicity in South Korea: A Population-Based Cohort Study
Authors: Ahn Seong Joon , Kim Jiyeong , Kwon Hyeon Yoon
Published: April 2024

(1) Background/Objectives: To investigate the nationwide screening practices and trends in tamoxifen retinal toxicity (tamoxifen retinopathy) in South Korea using national health insurance claims data. (2) Methods: A total of 43,848 patients who started tamoxifen therapy between 2015 and 2020 and had no prior ophthalmic diseases or other conditions requiring screening for retinopathy were included. The annual numbers of tamoxifen users and new initiators of tamoxifen therapy were assessed. The screening examinations were separated into baseline (first ophthalmic examination after tamoxifen administration) and subsequent monitoring examinations. The timing and modalities for the baseline and subsequent monitoring examinations performed between 2015 and 2021 were assessed in tamoxifen users. (3) Results: The annual number of tamoxifen users increased over the study period from 54,056 in 2015 to 81,720 in 2021. The number of patients who underwent ophthalmic examination after tamoxifen administration was 8961 (20.4%). Baseline screening was performed in 6.5% of patients within 1 year of use, and subsequent monitoring was performed in 27.8% of patients who underwent baseline screening. Funduscopy or fundus photography was performed most commonly for baseline screening and subsequent monitoring (99.0% and 98.6%, respectively), while optical coherence tomography was performed only in 21.9% and 29.6% of baseline and monitoring examinations, respectively. The average number of monitoring examinations per year was 0.68 ± 0.45. Although the annual percentage of patients receiving a baseline examination within 1 year gradually increased over time, the percentage of those with subsequent monitoring performed within 1 year was similar over the study period. (4) Conclusions: Our finding, appropriate screening in a small proportion of patients receiving tamoxifen, suggests the need to promote awareness among healthcare professionals and develop a standardized approach for screening for tamoxifen retinopathy.
A Residual N-Terminal Peptide Enhances Signaling of Depalmitoylated Hedgehog to the Patched Receptor
Authors: Ehlers Sophia F. , Manikowski Dominique , Steffes Georg , Ehring Kristina , Gude Fabian , Grobe Kay
Published: April 2024

During their biosynthesis, Sonic hedgehog (Shh) morphogens are covalently modified by cholesterol at the C-terminus and palmitate at the N-terminus. Although both lipids initially anchor Shh to the plasma membrane of producing cells, it later translocates to the extracellular compartment to direct developmental fates in cells expressing the Patched (Ptch) receptor. Possible release mechanisms for dually lipidated Hh/Shh into the extracellular compartment are currently under intense debate. In this paper, we describe the serum-dependent conversion of the dually lipidated cellular precursor into a soluble cholesteroylated variant (ShhC) during its release. Although ShhC is formed in a Dispatched- and Scube2-dependent manner, suggesting the physiological relevance of the protein, the depalmitoylation of ShhC during release is inconsistent with the previously postulated function of N-palmitate in Ptch receptor binding and signaling. Therefore, we analyzed the potency of ShhC to induce Ptch-controlled target cell transcription and differentiation in Hh-sensitive reporter cells and in the Drosophila eye. In both experimental systems, we found that ShhC was highly bioactive despite the absence of the N-palmitate. We also found that the artificial removal of N-terminal peptides longer than eight amino acids inactivated the depalmitoylated soluble proteins in vitro and in the developing Drosophila eye. These results demonstrate that N-depalmitoylated ShhC requires an N-peptide of a defined minimum length for its signaling function to Ptch.
Sub-Bottom Sediment Classification Employing a Multi-Attribute Temporal Convolutional Network
Authors: Liu Lei , Li Houpu , Bian Shaofeng , Zhu Junhui
Published: April 2024

Sub-bottom profile data have the potential to characterize sediment properties but are seldom used for offshore site investigations because of uncertainties in rock-physics models. Deep-learning techniques appear to be poised to play very important roles in our processing flows for the interpretation of geophysical data. In this paper, a novel deep learning-based method for this task is proposed in which a nonlinear mapping between the observed data and sediment types is learned using a multi-attribute temporal convolution network (MATCN). Firstly, empirical mode decomposition (EMD) is employed for the original data, and intrinsic mode functions (IMFs) with multiple time scales are generated. Based on different IMFs, instantaneous frequency (IF) data under different IMFs can be obtained, while instantaneous phase (IP) and instantaneous amplitude (IA) data are obtained based on the original data. IF, IA and IP data are called attribute data, and are highly related to the attenuation, reflection, and interior structure of the sediment. Thus, IA, IF, and IP are used as the inputs, and a 1D convolutional neural network (CNN) and a time convolution network (TCN) are used to extract sequential features. Different feature representations are then fused. Combining cross-entropy loss function and class-edge loss function, the network is encouraged to produce classified results with more continuous sediment distributions compared with the traditional loss function. The real-data experiments demonstrate that the proposed MATCN has achieved good performance with an F measure greater than 70% in all cases, and greater than 80% in most cases.
Enhancing Frontal Sinus Surgery: Assessing the Long-Term Impact of Free Grafts and Flaps in Draf III Procedures
Authors: Leventi Argyro , Chatzinakis Vasileios , Papargyriou Georgia Evangelia , Georgalas Christos
Published: April 2024

The frontal sinus medial drainage —Draf Type III (modified endoscopic Lothrop) procedure, has become a cornerstone in frontal sinus surgery over the last three decades. Despite its widespread acceptance, challenges such as restenosis and neo-ostium closure persist, prompting the exploration of various preventive techniques. In this retrospective study, we analyzed data from 111 patients who underwent the Draf III procedure between November 2015 and November 2023, with a mean follow-up period of 3 years and 11 months. Approximately two-thirds of patients (64%) had undergone previous sinus surgery and 16% a previous Draf III. Over half of the patients had inflammatory conditions, with the majority being chronic rhinosinusitis with nasal polyps (CRSwNP) (46%), while 15% were diagnosed with malignant sinonasal tumors, and 23% with benign sinonasal tumors, of which the commonest was osteoma, accounting for 14 cases. The mean follow-up period was 3 years and 11 months. We focused on evaluating the efficacy of mucosal flaps and free grafts in preventing neo-ostium closure. Although it appears that there is no statistically significant correlation between flap usage and the need for revision surgery or ostium patency maintenance overall, subgroup analysis highlighted the benefits of flap reconstruction in patients with chronic rhinosinusitis with nasal polyps. In this subgroup, the use of flaps or grafts reduced the rate of neo-ostium stenosis from 20% to 0% (p < 0.05). Overall revision rate was 11.7%—however this was 8% in patients without acute inflammation at the time of surgery and went up to 31% in the presence of pus in the frontal recess (p = 0.02). This study contributes to the existing literature by providing insights into long-term outcomes, the enduring effectiveness of interventions in frontal sinus surgery, and especially the importance of taking into account the underlying pathology when assessing long-term outcomes.
Chest Radiography Pearls in Select Adult Congenital Heart Disease
Authors: Schiavone William A. , Majdalany David S.
Published: April 2024

Congenital heart disease in adult patients (ACHD) includes individuals with native anatomic deformities and those who have benefited from corrective, ameliorative, or interventional heart and vascular interventions. Congenital heart disease is the most common birth defect, although with interventions most survive into adulthood. Newborns and children with complex congenital heart diseases that feature cyanosis fail to thrive, and once this is identified, heart failure can promptly undergo diagnostic evaluations and treatment. However, patients with simple congenital heart disease and subtle clinical signs and symptoms may escape diagnosis until adulthood or experience changes in their cardiac hemodynamics and physiology in settings such as pregnancy or newly diagnosed arrhythmias. The chest X-ray (CXR) is the most common X-ray among all radiological procedures. Individual features or a constellation of features on a CXR are often present in patients who have congenital heart disease. The ability to recognize these CXR features is a valuable skill for making the diagnosis of ACHD and for following these patients as they age, and can complement echocardiographic findings. When used well to diagnose ACHD, the CXR will be the sharpest arrow in the quiver.
Impact of COVID-19 on Pediatric Inflammatory Bowel Diseases—From Expectations to Reality
Authors: Trandafir Laura Mihaela , Spoiala Elena Lia , Ghiga Gabriela , Gimiga Nicoleta , Budescu Paula-Diana , Lupu Vasile Valeriu , Butnariu Lacramioara , Cojocaru Elena , Paduraru Gabriela
Published: April 2024

Viral infections have always been considered a threat to global health, with numerous outbreaks across time. Despite the relative recent experience with coronavirus-associated diseases such as severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), severe acute respiratory syndrome-2’s (SARS-CoV-2) continuous evolution displays a different behavior. With a tropism for both respiratory and digestive mucosa, coronavirus disease 2019 (COVID-19) and inflammatory bowel disease (IBD) seem to share a particular common background. Current literature offers evidence that viral alteration of the immune system, inflammatory intestinal tissue damage, increased intestinal permeability, incomplete viral clearance with viral antigen persistence, and intestinal dysbiosis, might explain SARS-CoV-2–IBD relationship in terms of etiopathogenesis and evolution. The hyperinflammatory state that both entities have in common explains the lack of success of current IBD therapy, raising the need for new personalized therapeutic options, with better outcomes for IBD and COVID-19 as well. This review aims to summarize the current available data on pediatric IBD evolution, management, and outcomes in the post-COVID period, with an emphasis on the particular aspects of the SARS-CoV-2–IBD relationship in children.
Conservation of Water Resources in a Botanic Garden
Authors: Washburn Chad E.
Published: April 2024

Water-resource challenges, encompassing both quality and quantity, pose significant threats to Florida’s ecosystems, especially in the face of climate change, rising sea levels, and rapid urbanization. This paper explores the innovative stormwater-management system implemented at Naples Botanical Garden as a model for addressing these challenges. The Garden’s approach, treating stormwater as a valuable resource, involves dry and wet retention areas, created lakes, and a unique River of Grass, mimicking natural ecosystems. This system not only mitigates flooding, but also effectively removes pollutants, recharges the aquifer, and provides a habitat for diverse wildlife. The paper emphasizes the economic, environmental, and social impacts of traditional stormwater-management practices in Florida. Naples Botanical Garden’s case serves as a guide for botanical gardens and zoos globally, showcasing the pivotal role these institutions can play in sustainable water-resource management. The collaborative design process involving landscape architects, engineers, and horticulturists ensures a holistic and aesthetically pleasing approach to stormwater management. The paper underscores the role of botanical gardens in promoting nature-based solutions, educating the public, and offering tangible steps for implementing similar systems worldwide. It can help guide regional adaptation strategies to manage stormwater as a resource.
Resilience Assessment of Historical and Cultural Cities from the Perspective of Urban Complex Adaptive Systems
Authors: Chen Tianyu , Bian Guangmeng , Wang Ziyi
Published: April 2024

Due to the increasingly complex global climatic environment and the rapid development of China’s urban construction, China’s historical and cultural cities are experiencing an external impact as well as internal fragility. Representing the capacity of the urban system to address impact and pressure, resilience can effectively guarantee the sustainable development of historical and cultural cities. A scientific and reasonable resilience assessment system can guide the resilience construction of historical and cultural cities in an effort to effectively counter the impact and pressure they face. Therefore, it is necessary to research the resilience of historical and cultural cities. On the basis of the complex adaptive system (CAS), and by applying multiple assessment indicators, this paper established a resilience assessment system for China’s historical and cultural cities, comprising 38 indicators in six dimensions, to analyze the characteristics and the influencing mechanisms of the resilience of the historical and cultural cities and to reveal the inherent logic underlying their complex presentation. Using six historical and cultural cities in east China as an example, the study applied the assessment system to assess and analyze the different resilience levels of the cities. The comprehensive resilience of Changzhou City obtained the highest score at 0.64, indicating a higher degree of resilience; the scores of Yantai City, Huzhou City, and Nantong City were 0.59, 0.54, and 0.50, respectively, representing moderate degrees of resilience; the scores of Zhongshan City and Quzhou City were 0.44 and 0.40, respectively, exhibiting a lower degree of resilience. Moreover, the factors that result in an unbalanced development of urban resilience were explored from the perspectives of economy, system, and culture. The paper contains some significance in guiding the development of the resilience of historical and cultural cities.
Research on the Decoupling Relationship between Transportation Land and Population Growth: A Case of Guangdong Province in China
Authors: He Junrui , Yang Senbin , Deng Shuhan , Ye Jianping , Chen Hongsheng
Published: April 2024

The imbalance between the population, transportation, and construction is an important factor affecting coordinated regional development in China. Using transportation land area and population data from 2010, 2015, and 2020 in 121 counties (cities and districts) of Guangdong Province, this study analyzes the spatiotemporal changes in transportation land and population structure. It explores the relationship between transportation land and population size changes using exploratory spatial data analysis (ESDA) and decoupling models. Research suggests the following: (1) From 2010 to 2020, the per capita transportation land in various districts and counties of Guangdong Province showed a hierarchical structure of “low center and high periphery”. Conversely, the proportion of transportation land to construction land displayed a “high center and low periphery” characteristic. (2) During the same period, the total amount of transportation land in the peripheral areas of the Pearl River Delta was higher than that in the PRD (the Pearl River Delta) and the gap widened each year. Meanwhile, the proportion of transportation land to construction land in the peripheral areas of the Pearl River Delta was consistently higher than that in the PRD. (3) From 2010 to 2015, the relationship between population growth and transportation land in Guangdong Province showed a hierarchical pattern, while from 2015 to 2020, the pattern became more complex, and regional imbalance intensified. The decoupling results of PRD showed weak decoupling and expanding negative decoupling from 2010 to 2020. The peripheral areas of the Pearl River Delta are characterized by strong negative decoupling and expanding negative decoupling. Research has shown that although Guangdong Province is one of the most developed provinces in China, the mismatch between transportation land and population size in economically underdeveloped areas has become increasingly severe, with changes in the provincial population distribution pattern. The decoupling phenomenon between the transportation land and population is very obvious, and the policy of achieving regional balanced development through transportation infrastructure construction needs to be reviewed.
Application of Time Series INSAR (SBAS) Method Using Sentinel-1 for Monitoring Ground Deformation of the Aegina Island (Western Edge of Hellenic Volcanic Arc)
Authors: Kalavrezou Ioanna-Efstathia , Castro-Melgar Ignacio , Nika Dimitra , Gatsios Theodoros , Lalechos Spyros , Parcharidis Issaak
Published: April 2024

This study employs advanced synthetic aperture radar (SAR) techniques, specifically the small baseline subset (SBAS) method, to analyze ground deformation dynamics on Aegina, a volcanic island within the Hellenic Volcanic Arc. Using Sentinel-1 satellite data spanning January 2016 to May 2023, this research reveals different deformation behaviors. The towns of Aegina and Saint Marina portray regions of stability, contrasting with central areas exhibiting subsidence rates of up to 1 cm/year. The absence of deformation consistent with volcanic activity on Aegina Island aligns with geological records and limited seismic activity, attributing the observed subsidence processes to settlement phenomena from past volcanic events and regional geothermal activity. These findings reinforce the need for continuous monitoring of the volcanic islands located in the Hellenic Volcanic Arc, providing important insights for local risk management, and contributing to our broader understanding of geodynamic and volcanic processes.
Diversity Levels under Different Grazing Intensities in Semi-Wet Grasslands
Authors: Avramidou Eleni , Karamichali Ioanna , Tsiripidis Ioannis , Abraham Eleni M.
Published: April 2024

The biodiversity of grasslands has been shaped by long-time interaction between natural processes and human activities, such as grazing. Traditional grazing management by animals contributes to the maintenance of high biodiversity in grasslands. However, changes in land use in recent years such as the abandonment of animal husbandry or intensification of grazing, pose a threat to grasslands biodiversity. The aim of the present study was to investigate the compositional and functional diversity in semi-humid grasslands of northern Greece under different grazing intensities. Three sites with similar vegetation and composition but different grazing intensities were selected in Taxiarchis University Forest of Mountain Holomontas. The three grazing intensities were (1) ungrazed, for more than 40 years, (2) moderate grazed, and (3) highly grazed. The ungrazed area showed an increase in vegetation cover and a decrease in bare ground. In comparison to grazed areas, the ungrazed one showed lower levels of grasses and legumes but higher abundances of woody and broad-leaved species. The highest values of all the studied diversity indices (Shannon–Wiener (H), Simpson Diversity Index, Jaccard, and species abundance) were recorded at moderate grazing intensity. Differences were recorded between the grazed and ungrazed areas in all of the quantitative traits (specific leaf area, leaf dry matter content, stem dry matter content, vegetative and reproductive plant height, leaf nitrogen, and phosphorus concentration). Protecting sensitive mountainous grasslands from both overgrazing and abandonment requires a balanced and sustainable management approach.
Evaluation of the Suitability of Street Vending Planning in Urban Public Space in the Post-COVID-19 Era
Authors: Zhang Xiaosi , Shao Jizhong
Published: April 2024

The COVID-19 pandemic has spurred the resurgence of the informal street vending economy. This revival has boosted the market economy but has also challenged urban governance. Recognizing street vending deregulation as both a stimulus for employment and a potential challenge to hygiene and safety, this study evaluates the suitability of street vending within Wuchang District of Wuhan, China. The methodology unfolds in four steps: constructing an indicator system based on a policy and literature review, a spatial quantitative analysis via GIS, weight allocation through expert consultation and entropy methods, and the identification of suitable areas as well as strategies. Findings reveal suitability clusters centered around key urban nodes, extending across central and mid-western zones through corridors like Zhongnan Road, demonstrating the potential for centralized management and economic permeation into surrounding areas. The analysis of land-use compatibility showed that commercial, park, and residential lands offer the highest suitability for vending activities, proposing management strategies tailored to enhance business in commercial areas, flexible configurations in residential zones, diverse development in parks, quality-oriented development in public squares, and strict control in scenic and tourist facilities. This study not only deepens our understanding of the geographical distribution of and factors influencing street vending, but also proposes integrated management strategies for vending in urban public spaces, fostering vibrant, inclusive, and sustainable urban environments.