bluuebunny/crossref_metadata_2025 huggingface dataset:
Simultaneous assessment and training of an upper-limb amputee using incremental machine-learning-based myocontrol: a single-case experimental design
Authors: Nowak Markus , Bongers Raoul M. , van der Sluis Corry K. , Albu-Schäffer Alin , Castellini Claudio
Published: April 2023

Background

Machine-learning-based myocontrol of prosthetic devices suffers from a high rate of abandonment due to dissatisfaction with the training procedure and with the reliability of day-to-day control. Incremental myocontrol is a promising approach as it allows on-demand updating of the system, thus enforcing continuous interaction with the user. Nevertheless, a long-term study assessing the efficacy of incremental myocontrol is still missing, partially due to the lack of an adequate tool to do so. In this work we close this gap and report about a person with upper-limb absence who learned to control a dexterous hand prosthesis using incremental myocontrol through a novel functional assessment protocol called SATMC (Simultaneous Assessment and Training of Myoelectric Control).

Methods

The participant was fitted with a custom-made prosthetic setup with a controller based on Ridge Regression with Random Fourier Features (RR-RFF), a non-linear, incremental machine learning method, used to build and progressively update the myocontrol system. During a 13-month user study, the participant performed increasingly complex daily-living tasks, requiring fine bimanual coordination and manipulation with a multi-fingered hand prosthesis, in a realistic laboratory setup. The SATMC was used both to compose the tasks and continually assess the participant’s progress. Patient satisfaction was measured using Visual Analog Scales.

Results

Over the course of the study, the participant progressively improved his performance both objectively, e.g., the time required to complete each task became shorter, and subjectively, meaning that his satisfaction improved. The SATMC actively supported the improvement of the participant by progressively increasing the difficulty of the tasks in a structured way. In combination with the incremental RR-RFF allowing for small adjustments when required, the participant was capable of reliably using four actions of the prosthetic hand to perform all required tasks at the end of the study.

Conclusions

Incremental myocontrol enabled an upper-limb amputee to reliably control a dexterous hand prosthesis while providing a subjectively satisfactory experience. The SATMC can be an effective tool to this aim.
Revolutionizing viral disease vaccination: the promising clinical advancements of non-replicating mRNA vaccines
Authors: Guo Xiao , Liu Dongying , Huang Yukai , Deng Youcai , Wang Ying , Mao Jingrui , Zhou Yuancheng , Xiong Yongai , Gao Xinghong
Published: April 2023

The mRNA vaccine technology was developed rapidly during the global pandemic of COVID-19. The crucial role of the COVID-19 mRNA vaccine in preventing viral infection also have been beneficial to the exploration and application of other viral mRNA vaccines, especially for non-replication structure mRNA vaccines of viral disease with outstanding research results. Therefore, this review pays attention to the existing mRNA vaccines, which are of great value for candidates for clinical applications in viral diseases. We provide an overview of the optimization of the mRNA vaccine development process as well as the good immune efficacy and safety shown in clinical studies. In addition, we also provide a brief description of the important role of mRNA immunomodulators in the treatment of viral diseases. After that, it will provide a good reference or strategy for research on mRNA vaccines used in clinical medicine with more stable structures, higher translation efficiency, better immune efficacy and safety, shorter production time, and lower production costs than conditional vaccines to be used as preventive or therapeutic strategy for the control of viral diseases in the future.
Measuring out-of-field dose to the hippocampus in common radiotherapy indications
Authors: Auerbach Hendrik , Dzierma Yvonne , Schürmann Michaela , Rübe Christian , Rübe Claudia E.
Published: April 2023

Background

The high susceptibility of the hippocampus region to radiation injury is likely the causal factor of neurocognitive dysfunctions after exposure to ionizing radiation. Repetitive exposures with even low doses have been shown to impact adult neurogenesis and induce neuroinflammation. We address the question whether the out-of-field doses during radiotherapy of common tumour entities may pose a risk for the neuronal stem cell compartment in the hippocampus.

Methods

The dose to the hippocampus was determined for a single fraction according to different treatment plans for the selected tumor entities: Point dose measurements were performed in an anthropomorphic Alderson phantom and the out-of-field dose to the hippocampus was measured using thermoluminescence dosimeters.

Results

For carcinomas in the head and neck region the dose exposure to the hippocampal region for a single fraction ranged from to 37.4 to 154.8 mGy. The hippocampal dose was clearly different for naso-, oro- and hypopharynx, with maximal values for nasopharynx carcinoma. In contrast, hippocampal dose levels for breast and prostate cancer ranged between 2.7 and 4.1 mGy, and therefore significantly exceeded the background irradiation level.

Conclusion

The mean dose to hippocampus for treatment of carcinomas in the head and neck region is high enough to reduce neurocognitive functions. In addition, care must be taken regarding the out of field doses. The mean dose is mainly related to scattering effects, as is confirmed by the data from breast or prostate treatments, with a very different geometrical set-up but similar dosimetric results.
Prophylactic antineoplastic activity of Toxoplasma gondii RH derived antigen against ehrlich solid carcinoma with evidence of shared antigens by comparative immunoblotting
Authors: Eissa Maha M. , Gaafar Maha R. , Younis Layla K. , Ismail Cherine A. , El Skhawy Nahla
Published: April 2023

Background

With cancer cases escalation, an urgent request to develop novel combating strategies arise. Pathogen-based cancer-immunotherapy is getting more consideration. Autoclaved parasitic antigens seem promising candidates, taking steadily their first steps. Our aim was to examine the prophylactic antineoplastic activity of autoclaved Toxoplasma vaccine (ATV) and to test for the shared antigen theory between Toxoplasma gondii and cancer cells.

Methods

Mice were immunized with ATV followed by Ehrlich solid carcinoma (ESC) inoculation. Tumor weight, volume, histopathology, and immunohistochemistry for CD8+ T cells, Treg cells and VEGF were assessed. In addition, the proposed shared antigen theory between parasites and cancer was also verified using SDS-PAGE and immunoblotting.

Results

Results revealed powerful prophylactic activity of ATV with 13.3% inhibition of ESC incidence, significant reduction in tumor weight and volume in ATV vaccinated mice. Immunologically, significantly higher CD8+T cells and lower FOXP3+ Treg cells surrounded and infiltrated ESC in ATV immunized mice with higher CD8+T/Treg cells ratio and significant antiangiogenic effect. Moreover, SDS-PAGE and immunoblotting showed four shared bands between Ehrlich carcinoma and ATV of approximate molecular weights 60, 26, 22 and 12.5 KDa.

Conclusion

Exclusively, we demonstrated a prophylactic antineoplastic activity of autoclaved Toxoplasma vaccine against ESC. Moreover, to the best of our knowledge this is the first report highlighting the existence of cross-reactive antigens between Toxoplasma gondi parasite and cancer cells of Ehrlich carcinoma.
Ablation of microglia does not alter circadian rhythm of locomotor activity
Authors: Matsui Futaba , Yamaguchi Sho T. , Kobayashi Riho , Ito Shiho , Nagashima Sakimi , Zhou Zhiwen , Norimoto Hiroaki
Published: April 2023

Microglia, as macrophages in the brain, are responsible for immune responses and synaptic remodeling. Although the function of microglia is regulated by circadian rhythms, it is still unclear whether microglia are involved in the generation and light entrainment of circadian rhythms of behavior. Here, we report that microglial depletion does not alter behavioral circadian rhythms. We depleted ~ 95% of microglia in the mouse brain by PLX3397, a CSF1R inhibitor, and analyzed the effect on the spontaneous behaviors of mice. We found that neither the free-running period under constant darkness nor light entrainment under jet-lag circumstances were influenced by the ablation of microglia. Our results demonstrate that the circadian rhythms of locomotor activity, an important output of the circadian clock in the brain, are likely a phenomenon not produced by microglia.
CAR-NK cell therapy for hematological malignancies: recent updates from ASH 2022
Authors: Huang Ruihao , Wen Qin , Zhang Xi
Published: April 2023

Chimeric antigen receptor (CAR)-NK cell therapy has the advantages of a low incidence of side effects and a low cost. However, the clinical outcomes are not satisfactory due to limited antitumor effects and a limited proliferative capacity. Recently, progress in CAR-NK cell therapy has been made in NK cell engineering, target design and combination with other agents to treat relapsed or refractory hematological malignancies, especially acute myeloid leukemia and multiple myeloma. This correspondence summarizes the preclinical and clinical updates for universal CAR-NK cell therapy reported at the ASH 2022 annual meeting.
Timing of bronchoscopy and application of scoring tools in children with severe pneumonia
Authors: Wu Xiangtao , Lu Weihong , Sang Xinquan , Xu Yali , Wang Tuanjie , Zhan Xiaowen , Hao Jie , Ren Ruijuan , Zeng Hanshi , Li Shujun
Published: April 2023

Background

There is still a lack of effective scoring criteria for assessing the severity of pulmonary infection associated with changes in the endobronchial lining of the bronchus in children. This study aimed to ascertain the timing and value of endoscopic scoring of fibreoptic bronchoscopy (FOB) and bronchoalveolar lavage (BAL) in children with severe pneumonia.

Method

The clinical data of 229 children with severe pneumonia treated with BAL in the Pediatric Intensive Care Unit of the First Affiliated Hospital of Xinxiang Medical University between November 2018 and December 2021 were collected. According to the severity of the disease, patients were divided into an invasive ventilation group and a non-invasive ventilation group, as well as an early BAL group (receiving BAL within 1 day of admission) and a late BAL group (receiving BAL 2 days after admission). A Student’s t-test, Chi-square test, receiver operating characteristic (ROC) curve and survival curve were used to analyse the bronchitis score, aetiology of BAL fluid and survival data.

Results

The scores of endoscopic mucosal oedema, erythema and pallor and the total score in the invasive ventilation group were higher than those in the non-invasive ventilation group (P < 0.05), and they were consistent with the Sequential Organ Failure Assessment (SOFA) scores. The secretion colour score was lower in the early BAL group than in the late BAL group (P < 0.05). On the bronchitis scores, which were evaluated using a ROC curve, the difference in the mucosal erythema, pallor, oedema and total score of the invasive and non-invasive groups was statistically significant (P < 0.05), which was consistent with the area under the ROC of the SOFA scores. Acute Physiology and Chronic Health Assessment II and SOFA scores after FOB were lower than those before treatment (P < 0.05). In terms of ICU hospitalisation days and total hospitalisation days, the time of the early FOB patients was shorter than that of the late FOB patients (P < 0.05). A total of 22 patients (9.61%) died. The Kaplan–Meier analysis and log-rank test showed that the survival rate of the non-invasive ventilation group was higher than that of the invasive ventilation group (P < 0.05).

Conclusion

This study found that FOB combined with BAL is an important method for the diagnosis and treatment of severe pneumonia. Early BAL can reduce hospitalisation and ICU time; however, it cannot improve the survival rate. The endoscopic score has a certain role to play in assessing the severity of pulmonary inflammation, but studies with a large sample are still needed to confirm this.
Multilevel interrogation of H3.3 reveals a primordial role in transcription regulation
Authors: Nabeel-Shah Syed , Garg Jyoti , Ashraf Kanwal , Jeyapala Renu , Lee Hyunmin , Petrova Alexandra , Burns James D. , Pu Shuye , Zhang Zhaolei , Greenblatt Jack F. , Pearlman Ronald E. , Lambert Jean-Philippe , Fillingham Jeffrey
Published: April 2023

Background

Eukaryotic cells can rapidly adjust their transcriptional profile in response to molecular needs. Such dynamic regulation is, in part, achieved through epigenetic modifications and selective incorporation of histone variants into chromatin. H3.3 is the ancestral H3 variant with key roles in regulating chromatin states and transcription. Although H3.3 has been well studied in metazoans, information regarding the assembly of H3.3 onto chromatin and its possible role in transcription regulation remain poorly documented outside of Opisthokonts.

Results

We used the nuclear dimorphic ciliate protozoan, Tetrahymena thermophila, to investigate the dynamics of H3 variant function in evolutionarily divergent eukaryotes. Functional proteomics and immunofluorescence analyses of H3.1 and H3.3 revealed a highly conserved role for Nrp1 and Asf1 histone chaperones in nuclear influx of histones. Cac2, a putative subunit of H3.1 deposition complex CAF1, is not required for growth, whereas the expression of the putative ortholog of the H3.3-specific chaperone Hir1 is essential in Tetrahymena. Our results indicate that Cac2 and Hir1 have distinct localization patterns during different stages of the Tetrahymena life cycle and suggest that Cac2 might be dispensable for chromatin assembly. ChIP-seq experiments in growing Tetrahymena show H3.3 enrichment over the promoters, gene bodies, and transcription termination sites of highly transcribed genes. H3.3 knockout followed by RNA-seq reveals large-scale transcriptional alterations in functionally important genes.

Conclusion

Our results provide an evolutionary perspective on H3.3’s conserved role in maintaining the transcriptional landscape of cells and on the emergence of specialized chromatin assembly pathways.
Sperm DNA methylation is predominantly stable in mice offspring born after transplantation of long-term cultured spermatogonial stem cells
Authors: Serrano Joana B. , Tabeling Nils C. , de Winter-Korver Cindy M. , van Daalen Saskia K. M. , van Pelt Ans M. M. , Mulder Callista L.
Published: April 2023

Background

Spermatogonial stem cell transplantation (SSCT) is proposed as a fertility therapy for childhood cancer survivors. SSCT starts with cryopreserving a testicular biopsy prior to gonadotoxic treatments such as cancer treatments. When the childhood cancer survivor reaches adulthood and desires biological children, the biopsy is thawed and SSCs are propagated in vitro and subsequently auto-transplanted back into their testis. However, culturing stress during long-term propagation can result in epigenetic changes in the SSCs, such as DNA methylation alterations, and might be inherited by future generations born after SSCT. Therefore, SSCT requires a detailed preclinical epigenetic assessment of the derived offspring before this novel cell therapy is clinically implemented. With this aim, the DNA methylation status of sperm from SSCT-derived offspring, with in vitro propagated SSCs, was investigated in a multi-generational mouse model using reduced-representation bisulfite sequencing.

Results

Although there were some methylation differences, they represent less than 0.5% of the total CpGs and methylated regions, in all generations. Unsupervised clustering of all samples showed no distinct grouping based on their pattern of methylation differences. After selecting the few single genes that are significantly altered in multiple generations of SSCT offspring compared to control, we validated the results with quantitative Bisulfite Sanger sequencing and RT-qPCRin various organs. Differential methylation was confirmed only for Tal2, being hypomethylated in sperm of SSCT offspring and presenting higher gene expression in ovaries of SSCT F1 offspring compared to control F1.

Conclusions

We found no major differences in DNA methylation between SSCT-derived offspring and control, both in F1 and F2 sperm. The reassuring outcomes from our study are a prerequisite for promising translation of SSCT to the human situation.
Diagnostic reference level quantities for adult chest and abdomen-pelvis CT examinations: correlation with organ doses
Authors: Costa Paulo Roberto , Tomal Alessandra , de Oliveira Castro Jullianna Cristina , Nunes Isabella Paziam Fernandes , Nersissian Denise Yanikian , Sawamura Márcio Valente Yamada , Leão Filho Hilton , Lee Choonsik
Published: April 2023

Objectives

To evaluate correlations between DRL quantities (DRLq) stratified into patient size groups for non-contrast chest and abdomen-pelvis CT examinations in adult patients and the corresponding organ doses.

Methods

This study presents correlations between DRLq  (CTDIvol, DLP and SSDE) stratified into patient size ranges and corresponding organ doses shared in four groups: inside, peripheral, distributed and outside. The demographic, technical and dosimetric parameters were used to identify the influence of these quantities in organ doses. A robust statistical method was implemented in order to establish these correlations and its statistical significance.

Results

Median values of the grouped organ doses are presented according to the effective diameter ranges. Organ doses in the regions inside the imaged area are higher than the organ doses in peripheral, distributed and outside regions, excepted to the peripheral doses associated with chest examinations. Different levels of statistical significance between organ doses and the DRLq were presented.

Conclusions

Correlations between DRLq and target-organ doses associated with clinical practice can support guidance’s to the establishment of optimization criteria. SSDE demonstrated to be significant in the evaluation of organ doses is also highlighted. The proposed model allows the design of optimization actions with specific risk-reduction results.
Paraganglioma in pregnancy, a mimic of preeclampsia: a case report
Authors: Lundholm Michelle D. , Marquard Jessica , Rao Pratibha PR
Published: April 2023

Background

The new presentation of pheochromocytoma or paraganglioma in pregnancy is very rare and can be life-threatening for mother and child.

Case presentation

We present the case of a 26-year-old gravida 3 para 2 otherwise healthy Caucasian woman at 34 weeks gestation who presented with new onset hypertension associated with headaches, dry heaves, diaphoresis, and palpitations. She was initially diagnosed with preeclampsia and treated with labetalol and an urgent cesarean section, delivering a healthy baby girl. The diagnosis of preeclampsia came into question when, 6 weeks postpartum, she continued to have hypertension with atypical features. Testing revealed metastatic paraganglioma associated with a succinate dehydrogenase B gene mutation. The patient was then started on alpha-adrenergic blockade and has had close blood pressure monitoring while discussion of advances therapies is ongoing.

Conclusion

This case demonstrates how paraganglioma/pheochromocytoma can be misdiagnosed as preeclampsia due to the overlapping features of new-onset hypertension late in pregnancy accompanied by headache and proteinuria. It is impractical to routinely screen for paraganglioma/pheochromocytoma in all pregnant patients diagnosed with preeclampsia due to the rarity of these tumors and the harm from high false-positive rates. Therefore, it is incumbent on the provider to have a high degree of suspicion for paraganglioma/pheochromocytoma when clinical features are unusual for preeclampsia, such as intermittent palpitations, diaphoresis, orthostatic hypotension, or hyperglycemia. Early detection of paraganglioma/pheochromocytoma with interventions to mitigate the risk of hypertensive crisis greatly reduce maternal and fetal mortality. Fortunately, our patient delivered a healthy baby and did not have any additional pregnancy complications despite the delay in her diagnosis.
Spheroids derived from the stromal vascular fraction of adipose tissue self-organize in complex adipose organoids and secrete leptin
Authors: Robledo Fermín , González-Hodar Lila , Tapia Pablo , Figueroa Ana-María , Ezquer Fernando , Cortés Víctor
Published: April 2023

Background

Adipose tissue-derived stromal vascular fraction (SVF) harbors multipotent cells with potential therapeutic relevance. We developed a method to form adipose spheroids (AS) from the SVF with complex organoid structure and enhanced leptin secretion upon insulin stimulation.

Methods

SVF was generated from the interscapular brown adipose tissue of newborn mice. Immunophenotype and stemness of cultured SVF were determined by flow cytometry and in vitro differentiation, respectively. Spheroids were generated in hanging drops and non-adherent plates and compared by morphometric methods. The adipogenic potential was compared between preadipocyte monolayers and spheroids. Extracellular leptin was quantified by immunoassay. Lipolysis was stimulated with isoprenaline and quantified by colorimetric methods. AS viability and ultrastructure were determined by confocal and transmission electron microscopy analyses.

Results

Cultured SVF contained Sca1 + CD29 + CD44 + CD11b- CD45- CD90- cells with adipogenic and chondrogenic but no osteogenic potential. Culture on non-adherent plates yielded the highest quantity and biggest size of spheroids. Differentiation of AS for 15 days in a culture medium supplemented with insulin and rosiglitazone resulted in greater Pparg, Plin1, and Lep expression compared to differentiated adipocytes monolayers. AS were viable and maintained leptin secretion even in the absence of adipogenic stimulation. Glycerol release after isoprenaline stimulation was higher in AS compared to adipocytes in monolayers. AS were composed of outer layers of unilocular mature adipocytes and an inner structure composed of preadipocytes, immature adipocytes and an abundant loose extracellular matrix.

Conclusion

Newborn mice adipose SVF can be efficiently differentiated into leptin-secreting AS. Prolonged stimulation with insulin and rosiglitazone allows the formation of structurally complex adipose organoids able to respond to adrenergic lipolytic stimulation.
Mesenchymal stromal/stem cell (MSC)-derived exosomes in clinical trials
Authors: Lotfy Ahmed , AboQuella Noha M. , Wang Hongjun
Published: April 2023

Mesenchymal stromal/stem cells (MSCs) are widely utilized in cell therapy because of their robust immunomodulatory and regenerative properties. Their paracrine activity is one of the most important features that contribute to their efficacy. Recently, it has been demonstrated that the production of various factors via extracellular vesicles, especially exosomes, governs the principal efficacy of MSCs after infusion in experimental models. Compared to MSCs themselves, MSC-derived exosomes (MSC-Exos) have provided significant advantages by efficiently decreasing unfavorable adverse effects, such as infusion-related toxicities. MSC-Exos is becoming a promising cell-free therapeutic tool and an increasing number of clinical studies started to assess the therapeutic effect of MSC-Exos in different diseases. In this review, we summarized the ongoing and completed clinical studies using MSC-Exos for immunomodulation, regenerative medicine, gene delivery, and beyond. Additionally, we summarized MSC-Exos production methods utilized in these studies with an emphasis on MSCs source, MSC-Exos isolation methods, characterization, dosage, and route of administration. Lastly, we discussed the current challenges and future directions of exosome utilization in different clinical studies as a novel therapeutic strategy.
Application of mesenchymal stem cell sheet for regeneration of craniomaxillofacial bone defects
Authors: Banimohamad-Shotorbani Behnaz , Karkan Sonia Fathi , Rahbarghazi Reza , Mehdipour Ahmad , Jarolmasjed Seyedhosein , Saghati Sepideh , Shafaei Hajar
Published: April 2023

Bone defects are among the most common damages in human medicine. Due to limitations and challenges in the area of bone healing, the research field has turned into a hot topic discipline with direct clinical outcomes. Among several available modalities, scaffold-free cell sheet technology has opened novel avenues to yield efficient osteogenesis. It is suggested that the intact matrix secreted from cells can provide a unique microenvironment for the acceleration of osteoangiogenesis. To the best of our knowledge, cell sheet technology (CST) has been investigated in terms of several skeletal defects with promising outcomes. Here, we highlighted some recent advances associated with the application of CST for the recovery of craniomaxillofacial (CMF) in various preclinical settings. The regenerative properties of both single-layer and multilayer CST were assessed regarding fabrication methods and applications. It has been indicated that different forms of cell sheets are available for CMF engineering like those used for other hard tissues. By tackling current challenges, CST is touted as an effective and alternative therapeutic option for CMF bone regeneration.
Benefits of pre-trained mono- and cross-lingual speech representations for spoken language understanding of Dutch dysarthric speech
Authors: Wang Pu , Van hamme Hugo
Published: April 2023

With the rise of deep learning, spoken language understanding (SLU) for command-and-control applications such as a voice-controlled virtual assistant can offer reliable hands-free operation to physically disabled individuals. However, due to data scarcity, it is still a challenge to process dysarthric speech. Pre-training (part of) the SLU model withsupervisedautomatic speech recognition (ASR) targets or withself-supervisedlearning (SSL) may help to overcome a lack of data, but no research has shown which pre-training strategy performs better for SLU on dysarthric speech and to which extent the SLU task benefits from knowledge transfer from pre-training with dysarthric acoustic tasks. This work aims to compare different mono- or cross-lingual pre-training (supervisedandunsupervised) methodologies and quantitatively investigates the benefits of pre-training for SLU tasks on Dutch dysarthric speech. The designed SLU systems consist of a pre-trained speech representations encoder and a SLU decoder to map encoded features to intents. Four types of pre-trained encoders, a mono-lingual time-delay neural network (TDNN) acoustic model, a mono-lingual transformer acoustic model, a cross-lingual transformer acoustic model (Whisper), and a cross-lingual SSL Wav2Vec2.0 model (XLSR-53), are evaluated complemented with three types of SLU decoders: non-negative matrix factorization (NMF), capsule networks, and long short-term memory (LSTM) networks. The acoustic analysis of the four pre-trained encoders are tested on Dutch dysarthric home-automation data with word error rate (WER) results to investigate the correlations of the dysarthric acoustic task (ASR) and the semantic task (SLU). By introducing the intelligibility score (IS) as a metric of the impairment severity, this paper further quantitatively analyzes dysarthria-severity-dependent models for SLU tasks.
$(H_{p}-L_{p})$-Type inequalities for subsequences of Nörlund means of Walsh–Fourier series
Authors: Baramidze David , Persson Lars-Erik , Tangrand Kristoffer , Tephnadze George
Published: April 2023

We investigate the subsequence $\{t_{2^{n}}f \}$
{

t

2
n

f
}
of Nörlund means with respect to the Walsh system generated by nonincreasing and convex sequences. In particular, we prove that a large class of such summability methods are not bounded from the martingale Hardy spaces $H_{p}$

H
p

to the space $\mathit{weak-}L_{p} $

w
e
a
k
−

L
p

for $0< p<1/(1+\alpha ) $
0
<
p
<
1
/
(
1
+
α
)
, where $0<\alpha <1$
0
<
α
<
1
. Moreover, some new related inequalities are derived. As applications, some well-known and new results are pointed out for well-known summability methods, especially for Nörlund logarithmic means and Cesàro means.
Melatonin promotes the development of the secondary hair follicles by regulating circMPP5
Authors: Diao Xiaogao , Yao Lingyun , Duan Tao , Qin Jiaxin , He Liwen , Zhang Wei
Published: April 2023

Background

The quality and yield of cashmere fibre are closely related to the differentiation and development of secondary hair follicles in the skin of cashmere goats. The higher the density of secondary hair follicles, the higher the quality and yield of cashmere from the fleece. Development of secondary hair follicles commences in the embryonic stage of life and is completed 6 months after birth. Preliminary experimental results from our laboratory showed that melatonin (MT) treatment of goat kids after their birth could increase the density of secondary hair follicles and, thus, improve the subsequent yield and quality of cashmere. These changes in the secondary hair follicles resulted from increases in levels of antioxidant and expression of anti-apoptotic protein, and from a reduction in apoptosis. The present study was conducted to explore the molecular mechanism of MT-induced secondary hair follicle differentiation and development by using whole-genome analysis.

Results

MT had no adverse effect on the growth performance of cashmere kids but significantly improved the character of the secondary hair follicles and the quality of cashmere, and this dominant effect continued to the second year. Melatonin promotes the proliferation of secondary hair follicle cells at an early age. The formation of secondary hair follicles in the MT group was earlier than that in the control group in the second year. The genome-wide data results involved KEGG analysis of 1044 DEmRNAs, 91 DElncRNAs, 1054 DEcircRNAs, and 61 DEmiRNAs which revealed that the mitogen-activated protein kinase (MAPK) signaling pathway is involved in the development of secondary hair follicles, with key genes (FGF2, FGF21, FGFR3, MAPK3 (ERK1)) being up-regulated and expressed. We also found that the circMPP5 could sponged miR-211 and regulate the expression of MAPK3.

Conclusions

We conclude that MT achieves its effects by regulating the MAPK pathway through the circMPP5 sponged the miR-211, regulating the expression of MAPK3, to induce the differentiation and proliferation of secondary hair follicle cells.

In addition there is up-regulation of expression of the anti-apoptotic protein causing reduced apoptosis of hair follicle cells. Collectively, these events increase the numbers of secondary hair follicles, thus improving the production of cashmere from these goats.
Potential roles of the rectum keystone microbiota in modulating the microbial community and growth performance in goat model
Authors: Wang Dangdang , Tang Guangfu , Zhao Lichao , Wang Mengya , Chen Luyu , Zhao Congcong , Liang Ziqi , Chen Jie , Cao Yangchun , Yao Junhu
Published: April 2023

Background

Ruminal microbiota in early life plays critical roles in the life-time health and productivity of ruminant animals. However, understanding of the relationship between gut microbiota and ruminant phenotypes is very limited. Here, the relationship between the rectum microbiota, their primary metabolites, and growth rate of a total of 76 young dairy goats (6-month-old) were analyzed, and then 10 goats with the highest or lowest growth rates respectively were further compared for the differences in the rectum microbiota, metabolites, and animal’s immune parameters, to investigate the potential mechanisms by which the rectum microbiota contributes to the health and growth rate.

Results

The analysis of Spearman correlation and microbial co-occurrence network indicated that some keystone rectum microbiota, including unclassified Prevotellaceae, Faecalibacterium and Succinivibrio, were the key modulators to shape the rectum microbiota and closely correlated with the rectum SCFA production and serum IgG, which contribute to the health and growth rate of young goats. In addition, random forest machine learning analysis suggested that six bacterial taxa in feces could be used as potential biomarkers for differentiating high or low growth rate goats, with 98.3% accuracy of prediction. Moreover, the rectum microbiota played more important roles in gut fermentation in early life (6-month-old) than in adulthood stage (19-month-old) of goats.

Conclusion

We concluded that the rectum microbiota was associated with the health and growth rate of young goats, and can be a focus on the design of the early-life gut microbial intervention.
“A calf cannot fail to pick a colour from its mother”: intergenerational transmission of trauma and its effect on reconciliation among post-genocide Rwandan youth
Authors: Kagoyire Marie Grace , Kangabe Jeannette , Ingabire Marie Chantal
Published: April 2023

Background

More than one million Rwandans were killed over a span of one hundred days during the 1994 genocide against the Tutsis. Many adult survivors were severely traumatized by the events, and young people, including those who were born after the genocide, have experienced similar genocide-related trauma. Building on a growing body of research on the generational transmission of trauma, our study addressed the following questions: (1) what are the possible mechanisms of trauma transmission from older generation to post-genocide Rwandan youth, and (2) what are the effects of intergenerational trauma on reconciliation processes in Rwanda.

Methods

A qualitative study was conducted in Rwanda among youth born after the genocide, with parents who survived the 1994 genocide against the Tutsis and among mental health and peace-building professionals. Individual interviews (IDIs) included 19 post-genocide descendants of survivors and six focus group discussions (FGDs) were conducted with 36 genocide survivor parents residing in Rwanda’s Eastern Province. Ten IDIs were also conducted with mental health and peace-building professionals in the capital city of Kigali. Respondents were recruited through five local organisations that work closely with survivors and their descendants. An inductive thematic analysis approach was used to analyse the data.

Results

Findings from this study suggest that the trauma experienced by genocide survivor parents is perceived by Rwandan youth, mental health and peace-building professionals, and survivor parents themselves to be transmitted from parent to child through human biology mechanisms, social patterns of silence and disclosure of genocide experiences, and children’s and youth’s everyday contact with a traumatized parent. Genocide-related trauma among survivor parents is seen as often being triggered by both life at home and the annual genocide commemoration events. Additionally, when transmitted to genocide survivor descendants, such trauma is understood to negatively affect their psychological and social well-being. Intergenerational trauma among youth with genocide survivor parents limits their involvement in post-genocide reconciliation processes. Findings specifically show that some youth avoid reconciliation with a perpetrator’s family due to mistrust as well as fear of re-traumatizing their own parents.
Psychometric properties of the Greek Version of the Traumatic Event Scale (TES) (Version A) among low-risk pregnant women
Authors: Varela Pinelopi , Lykeridou Aikaterini , Zervas Ioannis , Deltsidou Anna
Published: April 2023

Background

The Traumatic Event Scale (TES) is one of the most often used instruments for the assessment of the Posttraumatic Stress Disorder (PTSD) symptomatology during pregnancy which is linked with adverse effects. The aim of the study was to assess the psychometric properties of the TES (version A) in a sample of Greek pregnant women.

Methods

Two hundred one low risk pregnant women in their second or third trimester were invited to participate in the study. Participants completed a number of questionnaires including the Greek versions of TES-A, State-Trait Anxiety Inventory (STAI), Coping Orientations to Problems Experienced (Brief COPE), Perceived Stress Scale (PSS-10) and Edinburgh Postnatal Depression Scale (EPDS). Confirmatory factor analysis (CFA) was conducted in order to test how well the already TES-A five-factor model fits the data from Greece.

Results

Participants’ average age was 34.2 years (SD = 4.3 years). Through CFA the already five-factor structure of the TES-A (Anticipation of trauma, Intrusion, Avoidance, Resignation, Hyperstimulation) was applied to our sample. All five factors were significantly and positively correlated with each other. All Cronbach’s alpha were over 0.7, indicating acceptable reliability of the factors. Relatively convergent validity, all factors of the Greek version of the TES-A were significantly associated with stress, anxiety, depression and coping strategies.

Conclusion

The Greek version of TES-A is detected to be a valid and reliable instrument of prenatal Posttraumatic Stress Disorder (PTSD) symptomatology among low-risk Greek pregnant women.
New “iPARKO” finger extensor facilitation training device for chronic hemiplegic hand after stroke: development and verification of its effectiveness in healthy individuals
Authors: Nakamura Ai , Peichen Zhou , Morita Yoshifumi , Tanabe Hirofumi
Published: April 2023

After stroke, hemiplegic individuals have difficulty opening their hands by themselves due to paralysis. A finger extensor facilitation technique was therefore developed to increase the activity of the extensor muscle in hemiplegic individuals who cannot open their paralyzed hands by themselves. In our previous study, we created a training device prototype called the “PARKO”, that imitates the finger extensor facilitation technique; however, the activity of the extensor muscle using the device was much smaller than that of the finger extensor facilitation technique. In this study, we developed a new finger extensor facilitation training device, the “iPARKO”, to increase the activity of the extensor muscle during active training. We thus improved the structure of the PARKO device—including a modified method to apply force to the fingertips—and attached force sensors to the device to monitor these forces. To verify the effectiveness of the iPARKO device, we conducted active training using the device in healthy individuals. Comparing the results of the muscle activities between the PARKO and iPARKO devices among three healthy participants demonstrated that the iPARKO device increased the mean activity of the extensor muscle by 43.8%. Furthermore, the activity of the extensor muscle in two healthy individuals while moving the hand forward with the iPARKO device was 84% and 96% of the maximal voluntary contraction during the hand-opening motion without the iPARKO device.
Children's attitudes towards aging people-validation of an instrument
Authors: Fernandes Carla Sílvia Neves da Nova , Moreira Teresa , Galvão Joana
Published: April 2023

Purpose

It is essential to develop and improve instruments that measure and assess children's attitudes towards the elderly. Despite the degree of questions related to this topic, no tools validated for the Portuguese population on the subject were found in the literature. Consequently, this study aimed to translate and validate a scale designed to assess children’s attitudes towards the elderly (Todaro Scale) for the Portuguese population.

Design and methods

The method recommended by the literature for psychometric validation and evaluation of instruments was followed. In the conception of the study, the following stages were followed: translation of the ‘‘Todaro Scale’’, translation and back-translation by an independent translator, validation by an expert panel, pre-test and validation of the questionnaire, and testing the scale measurement properties. The convenience sample comprised 60 children between the ages of 4 and 6, of Portuguese nationality and residents of Portugal.

Results

The results obtained in the reliability and validity tests reveal a good internal consistency. The 14 items of the scale, and after the use of principal component analysis, there were grouped into five dimensions: social participation, personality characteristics; Health and Safety, physical and instrumental; cognitive and social appreciation.

Conclusions

The psychometric study confirms that the ‘‘Galvão Scale: children’s attitudes towards the elderly’’ includes the essential indicators and dimensions to ensure an adequate assessment of children’s attitudes towards the elderly. Practice implications: we believe this research could become an indispensable contribution to analysing, monitoring, and intervening to demystify existing ageing stereotypes.
The phylogenetic relationship among two species of genus Nebo (Scorpiones: Diplocentridae) from Saudi Arabia and Middle East
Authors: Alqahtani Abdulaziz R. , Alotaibi Noura J. , Aly Hamdy , Badry Ahmed
Published: April 2023

Background

The genus Nebo has been identified as a medically important scorpion species distributed across Arabia and the Middle East. However, its taxonomic status remains unclear.

Aim

The molecular phylogeny of two Nebo species from Saudi Arabia and comparative sequences from Palestine is presented based on the mitochondrial cytochrome oxidase subunit I (COI) gene.

Methodology

Scorpion specimens were collected from two different localities, mainly the Southern part of Saudi Arabia. Then, DNA was extracted, amplified using invertebrate universal primers, and sequenced to identify the COI gene. The obtained sequences were analyzed, and phylogenetic trees based on maximum parsimony, neighbor-joining, and Bayesian inference were constructed.

Results

The inferred phylogeny indicates the monophyletic status of the family Diplocentridae and its subfamily Nebinae and Diplocentrinae. Also, the phylogenetic analyses support the existence of interspecific and intraspecific variations among/ within Nebo hierichonticus and Nebo yemenensis which may indicate distinct species.

Conclusion

Further morphological studies with additional specimens from the Arabian Peninsula may reveal possible undiscovered and cryptic species.
Patients as research partners in preference studies: learnings from IMI-PREFER
Authors: Smith Meredith Y. , Janssens Rosanne , Jimenez-Moreno A. Cecilia , Cleemput Irina , Muller Mireille , Oliveri Serena , Simons Gwenda , Strammiello Valentina , Huys Isabelle , Falahee Marie
Published: April 2023

Background

There is growing recognition of the importance of patient and public stakeholder involvement (PPI) in patient preference research. However, limited evidence exists regarding the impact, barriers and enablers of PPI in preference studies. The Innovative Medicines Initiative (IMI)-PREFER project conducted a series of preference case studies which incorporated PPI.

Objective

To describe: (1) how PPI was operationalized in the PREFER case studies, (2) the impact of PPI, and (3) factors that served to impede and facilitate PPI.

Methods

We reviewed the PREFER final study reports to determine how patient partners were involved. We conducted a thematic framework analysis to characterize the impact of PPI and then administered a questionnaire to the PREFER study leads to identify barriers and facilitators to effective PPI.

Results

Eight PREFER case studies involved patients as research partners. Patient partners were involved in activities spanning all phases of the patient preference research process, including in study design, conduct and dissemination. However, the type and degree of patient partner involvement varied considerably. Positive impacts of PPI included improvements in the: (1) quality of the research and research process; (2) patient partner empowerment; (3) study transparency and dissemination of results; (4) research ethics, and (5) trust and respect between the research team and the patient community. Of the 13 barriers identified, the 3 most frequently reported were inadequate resources, insufficient time to fully involve patient partners, and uncertainty regarding how to operationalize the role of ‘patient partner. Among the 12 facilitators identified, the two most frequently cited were (1) having a clearly stated purpose for involving patients as research partners; and (2) having multiple patient partners involved in the study.

Conclusion

PPI had many positive impacts on the PREFER studies. Preference study leads with prior PPI experience reported a greater number of positive impacts than those with no such experience. In light of the numerous barriers identified, multi-faceted implementation strategies should be considered to support adoption, integration and sustainment of PPI within preference research. Additional case studies of patient partner involvement in preference research are needed as well to inform best practices in this area.
Association of C-reactive protein with histological, elastographic, and sonographic indices of non-alcoholic fatty liver disease in individuals with severe obesity
Authors: Jamialahmadi Tannaz , Bo Simona , Abbasifard Mitra , Sathyapalan Thozhukat , Jangjoo Ali , Moallem Seyed Adel , Almahmeed Wael , Ashari Sorour , Johnston Thomas P. , Sahebkar Amirhossein
Published: April 2023

Background

Inflammation is critical in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). hs-CRP, an inflammatory marker, is considered one of the prognostic predictors of hepatic damage progression in NAFLD in some studies.

Methods

We assessed the concordance of hs-CRP concentrations and liver steatosis, steatohepatitis, and fibrosis based on elastography, sonography and liver biopsy findings in patients with severe obesity undergoing bariatric surgery.

Results

Among 90 patients, 56.7% showed steatohepatitis and 8.9% severe fibrosis. Hs-CRP were significantly associated with liver histology in an adjusted regression model (OR 1.155, 95% CI 1.029–1.297, p = 0.014; OR 1.155, 1.029–1.297, p = 0.014; OR 1.130, 1.017–1.257, p = 0.024 for steatosis, steatohepatitis, and fibrosis, respectively). The ROC curve, a cutoff of hs-CRP = 7 mg/L, showed a reasonable specificity (76%) for detecting biopsy-proven fibrosis and steatosis.

Conclusion

hs-CRP was associated with any degree of histologically diagnosed liver damage, and it had a reasonable specificity for predicting biopsy-proven steatosis and fibrosis in obese individuals. Further studies are needed to identify non-invasive biomarkers that could predict NALFD progression due to the relevant health risks linked to liver fibrosis.
Detection of false data injection attacks on power systems using graph edge-conditioned convolutional networks
Authors: Chen Bairen , Wu Q. H. , Li Mengshi , Xiahou Kaishun
Published: April 2023

State estimation plays a vital role in the stable operation of modern power systems, but it is vulnerable to cyber attacks. False data injection attacks (FDIA), one of the most common cyber attacks, can tamper with measurement data and bypass the bad data detection (BDD) mechanism, leading to incorrect results of power system state estimation (PSSE). This paper presents a detection framework of FDIA for PSSE based on graph edge-conditioned convolutional networks (GECCN), which use topology information, node features and edge features. Through deep graph architecture, the correlation of sample data is effectively mined to establish the mapping relationship between the estimated values of measurements and the actual states of power systems. In addition, the edge-conditioned convolution operation allows processing data sets with different graph structures. Case studies are undertaken on the IEEE 14-bus system under different attack intensities and degrees to evaluate the performance of GECCN. Simulation results show that GECCN has better detection performance than convolutional neural networks, deep neural networks and support vector machine. Moreover, the satisfactory detection performance obtained with the data sets of the IEEE 14-bus, 30-bus and 118-bus systems verifies the effective scalability of GECCN.