bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolutionary perspective of Big tau structure: 4a exon variants of MAPT
Authors: Fischer Itzhak
Published: December 2022

The MAPT gene encoding the microtubule-associated protein tau can generate multiple isoforms by alternative splicing giving rise to proteins which are differentially expressed in specific areas of the nervous system and at different developmental stages. Tau plays important roles in modulating microtubule dynamics, axonal transport, synaptic plasticity, and DNA repair, and has also been associated with neurodegenerative diseases (tauopathies) including Alzheimer’s disease and frontotemporal dementia. A unique high-molecular-weight isoform of tau, originally found to be expressed in the peripheral nervous system and projecting neurons, has been termed Big tau and has been shown to uniquely contain the large exon 4a that significantly increases the size and 3D structure of tau. With little progress since the original discovery of Big tau, more than 25 years ago, we have now completed a comprehensive comparative study to analyze the structure of the MAPT gene against available databases with respect to the composition of the tau exons as they evolved from early vertebrates to primates and human. We focused the analysis on the evolution of the 4a exon variants and their homology relative to humans. We discovered that the 4a exon defining Big tau appears to be present early in vertebrate evolution as a large insert that dramatically changed the size of the tau protein with low sequence conservation despite a stable size range of about 250aa, and in some species a larger 4a-L exon of 355aa. We suggest that 4a exon variants evolved independently in different species by an exonization process using new alternative splicing to address the growing complexities of the evolving nervous systems. Thus, the appearance of a significantly larger isoform of tau independently repeated itself multiple times during evolution, accentuating the need across vertebrate species for an elongated domain that likely endows Big tau with novel physiological functions as well as properties related to neurodegeneration.
Mechanistic insights into the pleiotropic effects of butyrate as a potential therapeutic agent on NAFLD management: A systematic review
Authors: Amiri Parichehr , Arefhosseini Sara , Bakhshimoghaddam Farnush , Jamshidi Gurvan Hannah , Hosseini Seyed Ahmad
Published: December 2022

Non-alcoholic fatty liver disease (NAFLD) is one of the most common chronic diseases worldwide. As a multifaceted disease, NAFLD’s pathogenesis is not entirely understood, but recent evidence reveals that gut microbiota plays a significant role in its progression. Butyrate, a gut microbiota metabolite, has been reported to have hepato-protective effects in NAFLD animal models. The purpose of this systematic review is to determine how butyrate affects the risk factors for NAFLD. Searches were conducted using relevant keywords in electronic databases up to March 2022. According to the evidence presented in this study, butyrate contributes to a wide variety of biological processes in the gut–liver axis. Its beneficial properties include improving intestinal homeostasis and liver health as well as anti-inflammatory, metabolism regulatory and anti-oxidative effects. These effects may be attributed to butyrate’s ability to regulate gene expression as an epigenetic modulator and trigger cellular responses as a signalling molecule. However, the exact underlying mechanisms remain unclear. Human trials have not been performed on the effect of butyrate on NAFLD, so there are concerns about whether the results of animal studies can be translated to humans. This review summarises the current knowledge about the properties of butyrate, particularly its potential effects and mechanisms on liver health and NAFLD management.
Continuous glucose monitoring reveals similar glycemic variability in individuals with obesity despite increased HOMA-IR
Authors: Cooper Dylan J. , Zarabi Sharon , Farrand Brianna , Becker Amanda , Roslin Mitchell
Published: December 2022

Background/aims

Continuous glucose monitoring is a well-tolerated and versatile tool for management of diabetes and metabolic disease. While its use appears to be feasible to monitor glycemic profiles in diabetics, there is a paucity of data in individuals with obesity and normal glucose tolerance. The aim of this study is to investigate glucose fluctuations and insulin resistance patterns in normoglycemic participants with obesity vs. without obesity and contextualize these results against leading models for obesity.

Materials and methods

We designed a prospective, observational pilot study of two cohorts including 14 normoglycemic participants with obesity and 14 normoglycemic participants without obesity. Participants were monitored with continuous glucose monitoring (CGM) for five consecutive days. Insulin resistance levels were measured and glucometric data were extracted from CGM for all participants.

Results

Fasting serum insulin and homeostasis model assessment of insulin resistance (HOMA-IR) were significantly higher in the group with obesity (P < 0.05). While the group with obesity had a higher mean blood glucose (MBG), mean amplitude of glycemic excursions (MAGE), and continuous overall glycemic action-1 h (CONGA-1), these differences were not significant. On univariate linear regression, insulin resistance (HOMA-IR) was associated with body mass index (BMI), waist circumference (WC), cohort with obesity, cohort consuming a high glycemic diet, hemoglobin A1c (HbA1c), and fasting insulin levels. WC and fasting insulin levels remained predictors of HOMA-IR in our multivariable model.

Conclusion

While there is much excitement surrounding the use of commercial CGM products in obesity management, our results suggest that fasting insulin and HOMA-IR values may be more clinically useful than CGM data alone.
Distinct gene mutation profiles among multiple and single primary lung adenocarcinoma
Authors: Wang Yadong , Wang Guanghui , Zheng Haotian , Liu Jichang , Ma Guoyuan , Huang Gemu , Song Qingtao , Du Jiajun
Published: December 2022

With the development of technologies, multiple primary lung cancer (MPLC) has been detected more frequently. Although large-scale genomics studies have made significant progress, the aberrant gene mutation in MPLC is largely unclear. In this study, 141 and 44 lesions from single and multiple primary lung adenocarcinoma (SP- and MP-LUAD) were analyzed. DNA and RNA were extracted from formalin-fixed, paraffin-embedded tumor tissue and sequenced by using the next-generation sequencing-based YuanSu450TM gene panel. We systematically analyzed the clinical features and gene mutations of these lesions, and found that there were six genes differently mutated in MP-LUAD and SP-LUAD lesions, including RBM10, CDK4, ATRX, NTRK1, PREX2, SS18. Data from the cBioPortal database indicated that mutation of these genes was related to some clinical characteristics, such as TMB, tumor type, et al. Besides, heterogeneity analysis suggested that different lesions could be tracked back to monophyletic relationships. We compared the mutation landscape of MP-LUAD and SP-LUAD and identified six differentially mutated genes (RBM10, CDK4, ATRX, NTRK1, PREX2, SS18), and certain SNV loci in TP53 and EGFR which might play key roles in lineage decomposition in multifocal samples. These findings may provide insight into personalized prognosis prediction and new therapies for MP-LUAD patients.
SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis
Authors: Tan Tze Guan , Zybina Yulia , McKenna Cooper , Olow Aleksandra , Rukmini Subhadra Jayaraman , Wong Michael Thomas , Sadekova Svetlana , Chackerian Alissa , Bauché David
Published: December 2022

Introduction

Colorectal cancer (CRC) is largely refractory to currently available immunotherapies such as blockade of programmed cell death protein-1 (PD-1).

Results

In this study, we identified SPATA2 and its protein partner CYLD as novel regulators of CXC-ligand 10 (CXCL10), a T-cell-attractant chemokine, in CRC. By specifically deleting SPATA2 and CYLD in human and mouse CRC cell lines, we showed that these two proteins inhibit STAT1 accumulation and activation and subsequently CXCL10 expression in tumor cells. At steady-state, STAT1 is highly ubiquitinated in a SPATA2/CYLD-dependent manner. Finally, we demonstrated that tumor-specific deletion of SPATA2 and CYLD enhances anti-PD-1 response in vivo.

Discussion

Our data suggest that SPATA2 and CYLD represent two potential novel targets for treatment of immune-excluded, PD-1-resistant tumors.
Photodynamic therapy in glioblastoma: Detection of intraoperative inadvertent 5-ALA mediated photodynamic therapeutical effect after gross total resection
Authors: Ferrés Abel , Di Somma Alberto , Mosteiro Alejandra , Topczewski Thomaz Eduardo , Roldán Pedro , Pedrosa Leire , Diao Diouldé , Pineda Estela , Sierra Àngels , Enseñat Joaquim , González-Sánchez José Juan
Published: December 2022

Introduction

Glioblastoma (GBM) remains the most frequent and lethal primary brain tumor in adults, despite advancements in surgical resection techniques and adjuvant chemo- and radiotherapy. The most frequent recurrence pattern (75-90%) occurs in the form of continuous growth from the border of the surgical cavity, thus emphasizing the need for locoregional tumor control. Fluorescence-guided surgical resection using 5-ALA has been widely implemented in surgical protocols for such tumors. Recent literature also highlights the applicability of 5-ALA-mediated photodynamic therapy to obtain locoregional tumor control further. This study aims to identify if 5-ALA mediated photodynamic therapeutic effect after gross total glioblastoma resection has inadvertently occurred due to the exposition of protoporphyrin IX charged peripheral tumoral cells to operative room light sources.

Methods

Of 146 patients who were intervened from glioblastoma between 2015 and 2020, 33 were included in the present study. Strict gross total resection (without supralocal resection) had been accomplished, and adjuvant chemoradiotherapy protocol was administered. Two comparison groups were created regarding the location of the recurrence (group A: up to 1 centimeter from the surgical cavity, and group B: beyond 1 centimeter from the surgical cavity). The cutoff point was determined to be 1 centimeter because of the visible light penetrance to the normal brain tissue.

Results

In univariate analysis, both groups only differed regarding 5-ALA administration, which was significantly related to a minor relative risk of presenting the recurrence within the first centimeter from the surgical cavity (Relative Risk = 0,655 (95% CI 0,442-0,970), p-value=0,046). Results obtained in univariate analysis were corroborated posteriorly in multivariate analysis (RR=0,730 (95% CI 0,340-0,980), p=0,017).

Discussion

In the present study, a probable inadvertent 5-ALA photodynamic therapeutical effect has been detected in vivo. This finding widely opens the door for further research on this promising theragnostic tool.
Case report: Clinical success targeting BRAF-mutated, hormone receptor positive, HER2- negative advanced breast cancer patient with BRAF-inhibitor plus MEK- inhibitor
Authors: López de Sá Alfonso , de Luna Alicia , Antoñanzas Mónica , García-Barberán Vanesa , Moreno-Anton Fernando , García-Sáenz Jose A.
Published: December 2022

Background: Hormone receptor-positive, human epidermal growth factor 2-negative advanced breast cancer patients have had a wide range of therapeutical options since the incorporation of targeted therapies alongside classic chemotherapy. However, because of their disease, virtually all patients will eventually experience disease progression that might compromise their lives. Thriving investigation regarding molecular therapies has provided clinicians with new options for the treatment of many cancer patients. Dabrafenib and trametinib combination has proven useful in treating malignant melanoma patients harboring a BRAF V600E mutation, improving progression-free survival and overall survival, and it has been tested in other tumors. Here we report the case of a metastatic breast cancer patient harboring a BRAF V600E mutation that achieved complete response with dabrafenib and trametinib combination.
Functional compounds of ginseng and ginseng-containing medicine for treating cardiovascular diseases
Authors: Liu Lanchun , Hu Jun , Mao Qiyuan , Liu Chao , He Haoqiang , Hui Xiaoshan , Yang Guang , Qu Peirong , Lian Wenjing , Duan Lian , Dong Yan , Pan Juhua , Liu Yongmei , He Qingyong , Li Jun , Wang Jie
Published: December 2022

Ginseng (Panax ginseng C.A.Mey.) is the dry root and rhizome of the Araliaceae ginseng plant. It has always been used as a tonic in China for strengthening the body. Cardiovascular disease is still the main cause of death in the world. Some studies have shown that the functional components of ginseng can regulate the pathological process of various cardiovascular diseases through different mechanisms, and its formulation also plays an irreplaceable role in the clinical treatment of cardiovascular diseases. Therefore, this paper elaborates the current pharmacological effects of ginseng functional components in treating cardiovascular diseases, summarizes the adverse reactions of ginseng, and sorts out the Chinese patent medicines containing ginseng formula which can treat cardiovascular diseases.
Network pharmacological analysis of Xuefu Zhuyu decoction in the treatment of atherosclerosis
Authors: Yuan Jinxia , Yan Fei , Li Wei , Yuan Guoliang
Published: December 2022

Objective: Using a network pharmacological approach, this study will evaluate the effect of Xuefu Zhuyu Decoction in the treatment of atherosclerosis.

Methods: The data were imported into the STRING database to construct a protein-protein interaction network, and the network topology was analysed with the Bisogenet plug-in by Cytoscape 3.7.2. Using the R language Bioconductor platform, Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis for potential targets of Xuefu Zhuyu Decoction in the treatment of atherosclerosis were performed, and import the results were imported into Cytoscape 3.7.2. To map the results and create a KEGG network diagram, we used Cytoscape 3.7.2 for analysis.

Results: A total of 91 chemical components and 1320 disease targets were obtained, including 138 cross-targets. TNF, AKT1 and ALB were identified as important targets, and Gene Ontology functional analysis indicated that biological process was the primary cause of oxidative stress. The primary action of molecular function is binding. KEGG has explored and enriched 149 signalling pathways, including the AGE-RAGE signalling system and the TNF signalling network. According to a study involving molecular docking, quercetin and β-carotene have a strong binding affinity for AKT1 and ALB.

Conclusion: The potential of Xuefu Zhuyu Decoction to treat atherosclerosis through multiple components and targets provides a way to further study its mechanism.
A new conjugate gradient algorithm for noise reduction in signal processing and image restoration
Authors: Huang Pan , Liu Kaiping
Published: December 2022

Noise-reduction methods are an area of intensive research in signal processing. In this article, a new conjugate gradient method is proposed for noise reduction in signal processing and image restoration. The superiority of this method lies in its employment of the ideas of accelerated conjugate gradient methods in conjunction with a new adaptive method for choosing the step size. In this work, using some assumptions, the weak convergence of the designed method was established. As example applications, we implemented our method to solve signal-processing and image-restoration problems. The results of our numerical simulations demonstrate the effectiveness and superiority of the new approach.
Constructing spin-structured focal fields for chiral-sensitive trapping with dielectric metalens
Authors: Li Bingjie , Xin Yujie , Guo Xuyue , Fan Xinhao , Liu Sheng , Zhao Jianlin , Li Peng
Published: December 2022

Engineering the chiral field is crucial for the flexible manipulation of chiral particles. Some complex optical setups for constructing spin-structured fields have been well developed to sort particles with opposite chiralities toward opposite transversal directions. In this work, we demonstrate the robust construction of a class of focal fields that possess laterally variant optical spin angular momentum by using the monolayer dielectric metalens. By utilizing the simultaneous modulation capacity of the phase and polarization of the dielectric metalens, we can establish a line focus with laterally tailored gradient optical helicity. The focusing property of this metalens and the polarization structure of the focal field are theoretically analyzed using a hybrid vector-focusing model and experimentally demonstrated by NA = 0.2 and 0.5 samples. We illustrate that this type of gradient helicity offers opportunities to induce a chirality-sensitive lateral force for chiral particles.
Direction-dependent non-Hermitian skin effect in modulated photonic waveguide arrays
Authors: Wu Yanting , Zou Jingwen , Jiang Chuang , Li Xiaohong , Ke Shaolin
Published: December 2022

Non-Hermitian skin effect (NHSE), where huge modes are accumulated at system boundaries, offers new possibility for steering the transport and localization of light by non-Hermiticity. Here, the direction-dependent NHSE is proposed in a photonic waveguide array via spatially complex modulation, where the skin modes tend to localize at different boundaries for opposite propagation directions. We utilize complex modulation to arouse anisotropic coupling between symmetric and anti-symmetric modes in multimode waveguides and further match the refractive index of adjacent waveguides. In this way, a non-Hermitian Su–Schrieffer–Heeger (SSH) lattice that supports NHSE is achieved. In particular, the anisotropic coupling is highly unidirectional. For forward direction, it allows mode conversion from antisymmetric modes to symmetric modes. However, the process is forbidden for backward direction. As a result, the skin modes tend to locate at lower boundary for forward propagation but the localization direction is reversed for backward injection. Our results provide a potential platform to investigate NHSE on photonic chips and may find applications in non-magnetic unidirectional devices.
MSDenoiser: Muti-step adaptive denoising framework for super-resolution image from single molecule localization microscopy
Authors: Feng Qianghui , Song Qihang , Yan Meng , Huang Zhen Li , Wang Zhengxia
Published: December 2022

Recent developments in single-molecule localization microscopy (SMLM) enable researchers to study macromolecular structures at the nanometer scale. However, due to the complexity of imaging process, there are a variety of complex heterogeneous noises in SMLM data. The conventional denoising methods in SMLM can only remove a single type of noise. And, most of these denoising algorithms require manual parameter setting, which is difficult and unfriendly for biological researchers. To solve these problems, we propose a multi-step adaptive denoising framework called MSDenoiser, which incorporates multiple noise reduction algorithms and can gradually remove heterogeneous mixed noises in SMLM. In addition, this framework can adaptively learn algorithm parameters based on the localization data without manually intervention. We demonstrate the effectiveness of the proposed denoising framework on both simulated data and experimental data with different types of structures (microtubules, nuclear pore complexes and mitochondria). Experimental results show that the proposed method has better denoising effect and universality.
Drought stress modifies the community structure of root-associated microbes that improve Atractylodes lancea growth and medicinal compound accumulation
Authors: Wang Hongyang , Wang Yuefeng , Kang Chuanzhi , Wang Sheng , Zhang Yan , Yang Guang , Zhou Li , Xiang Zengxu , Huang Luqi , Liu Dahui , Guo Lanping
Published: December 2022

Atractylodes lancea is an important medicinal plant in traditional Chinese medicine, its rhizome is rich of volatile secondary metabolites with medicinal values and is largely demanded in modern markets. Currently, supply of high-yield, high-quality A. lancea is mainly achieved via cultivation. Certain soil microbes can benefit plant growth, secondary metabolism and induce resistance to environmental stresses. Hence, studies on the effects of soil microbe communities and isolates microorganisms on A. lancea is extremely meaningful for future application of microbes on cultivation. Here we investigated the effects of the inoculation with an entire soil microbial community on the growth, resistance to drought, and accumulation of major medicinal compounds (hinesol, β-eudesmol, atractylon and atractylodin) of A. lancea. We analyzed the interaction between A. lancea and the soil microbes at the phylum and genus levels under drought stress of different severities (inflicted by 0%, 10% and 25% PEG6000 treatments). Our results showed that inoculation with soil microbes promoted the growth, root biomass yield, medicinal compound accumulation, and rendered drought-resistant traits of A. lancea, including relatively high root:shoot ratio and high root water content under drought. Moreover, our results suggested drought stress was more powerful than the selectivity of A. lancea in shaping the root-associated microbial communities; also, the fungal communities had a stronger role than the bacterial communities in protecting A. lancea from drought. Specific microbial clades that might have a role in protecting A. lancea from drought stress were identified: at the genus level, the rhizospheric bacteria Bacillus, Dylla and Actinomadura, and rhizospheric fungi Chaetomium, Acrophialophora, Trichoderma and Thielava, the root endophytic bacteria Burkholderia-Caballeronia-Paraburkholderia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Dylla and Actinomadura, and the root endophytic fungus Fusarium were closely associated with A. lancea under drought stress. Additionally, we acquired several endophytic Paenibacillus, Paraburkholderia and Fusarium strains and verified they had differential promoting effects on the medicinal compound accumulation in A. lancea root. This study reports the interaction between A. lancea and soil microbe communities under drought stress, and provides insights for improving the outcomes in A. lancea farming via applying microbe inoculation.
Insights into the expression of DNA (de)methylation genes responsive to nitric oxide signaling in potato resistance to late blight disease
Authors: Drozda Andżelika , Kurpisz Barbara , Guan Yufeng , Arasimowicz-Jelonek Magdalena , Plich Jarosław , Jagodzik Przemysław , Kuźnicki Daniel , Floryszak-Wieczorek Jolanta
Published: December 2022

Our previous study concerning the pathogen-induced biphasic pattern of nitric oxide (NO) burst revealed that the decline phase and a low level of NO, due to S-nitrosoglutathione reductase (GSNOR) activity, might be decisive in the upregulation of stress-sensitive genes via histone H3/H4 methylation in potato leaves inoculated with avr P. infestans. The present study refers to the NO-related impact on genes regulating DNA (de)methylation, being in dialog with histone methylation. The excessive amounts of NO after the pathogen or GSNO treatment forced the transient upregulation of histone SUVH4 methylation and DNA hypermethylation. Then the diminished NO bioavailability reduced the SUVH4-mediated suppressive H3K9me2 mark on the R3a gene promoter and enhanced its transcription. However, we found that the R3a gene is likely to be controlled by the RdDM methylation pathway. The data revealed the time-dependent downregulation of the DCL3, AGO4, and miR482e genes, exerting upregulation of the targeted R3a gene correlated with ROS1 overexpression. Based on these results, we postulate that the biphasic waves of NO burst in response to the pathogen appear crucial in establishing potato resistance to late blight through the RdDM pathway controlling R gene expression.
Comprehensive transcriptome analysis reveals heat-responsive genes in flowering Chinese cabbage (Brassica campestris L. ssp. chinensis) using RNA sequencing
Authors: Ikram Muhammad , Chen Jingfang , Xia Yanshi , Li Ronghua , Siddique Kadambot H. M. , Guo Peiguo
Published: December 2022

Flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee, 2n=20, AA) is a vegetable species in southern parts of China that faces high temperatures in the summer and winter seasons. While heat stress adversely impacts plant productivity and survival, the underlying molecular and biochemical causes are poorly understood. This study investigated the gene expression profiles of heat-sensitive (HS) ‘3T-6’ and heat-tolerant (HT) ‘Youlu-501’ varieties of flowering Chinese cabbage in response to heat stress using RNA sequencing. Among the 37,958 genes expressed in leaves, 20,680 were differentially expressed genes (DEGs) at 1, 6, and 12 h, with 1,078 simultaneously expressed at all time points in both varieties. Hierarchical clustering analysis identified three clusters comprising 1,958, 556, and 591 down-regulated, up-regulated, and up- and/or down-regulated DEGs (3205 DEGs; 8.44%), which were significantly enriched in MAPK signaling, plant–pathogen interactions, plant hormone signal transduction, and brassinosteroid biosynthesis pathways and involved in stimulus, stress, growth, reproductive, and defense responses. Transcription factors, including MYB (12), NAC (13), WRKY (11), ERF (31), HSF (17), bHLH (16), and regulatory proteins such as PAL, CYP450, and photosystem II, played an essential role as effectors of homeostasis, kinases/phosphatases, and photosynthesis. Among 3205 DEGs, many previously reported genes underlying heat stress were also identified, e.g., BraWRKY25, BraHSP70, BraHSPB27, BraCYP71A23, BraPYL9, and BraA05g032350.3C. The genome-wide comparison of HS and HT provides a solid foundation for understanding the molecular mechanisms of heat tolerance in flowering Chinese cabbage.
Metabolism of Scenedesmus obliquus cultivated with raw plant substrates
Authors: Schambach Jenna Y. , Kruse Colin P. S. , Kitin Peter , Mays Wittney , Hunt Christopher G. , Starkenburg Shawn R. , Barry Amanda N.
Published: December 2022

The potential benefits of adding raw, non-food, lignocellulosic plant material as a carbon source for mixotrophic growth of microalgae have previously been demonstrated. This approach has advantages over using traditional carbon sources like glucose or acetate due to wide-spread plant biomass availability and substrate recalcitrance to bacterial contamination. Here, we report the overall growth characteristics and explore the metabolic patterns of Scenedesmus obliquus cultured in the presence raw plant substrate. An initial screen of plant substrate candidates showed an increase in specific growth rate and biomass accumulation when S. obliquus was cultured in the presence of switchgrass or yard waste compared to media alone. We observed a near doubling of microalgal dry weight when S. obliquus was grown with 0.2% (w/v) switchgrass under ambient CO2. Scanning electron microscopy (SEM) of corn stem after S. obliquus cultivation exhibited substantial phloem degradation. Transcriptomic analyses of S. obliquus during mid- and late-log phase growth revealed a dynamic metabolic landscape within many KEGG pathways. Notably, differential expression was observed for several potential glycosyl hydrolases. We also investigated the influence of switchgrass on the growth of S. obliquus at 50 L volume in mini raceway ponds to determine the scalability of this approach.
The impact of computer–assisted technology on literacy acquisition during COVID-19-related school closures: Group–level effects and predictors of individual–level outcomes
Authors: Richter Caroline G. , Siegelman Noam , Mahaffy Kelly , Van Den Bunt Mark , Kearns Devin M. , Landi Nicole , Sabatini John , Pugh Kenneth , Hoeft Fumiko
Published: December 2022

Introduction

The COVID-19 pandemic led to school closure and loss of in-person instruction during the 2019–2020 academic year across the United States, which had a profound impact on the reading development of beginning readers. In this study we tested if a research-informed educational technology (EdTech) program–GraphoLearn–could help alleviate the COVID-19 slide. We also sought to understand the profiles of children who benefitted most from this EdTech program.

Methods

We tested participants’ (N = 172 K-2 children) early literacy skills using a standardized measure (STAR) before and after playing GraphoLearn, and used the pre to post difference as the dependent variable. We first compared children’s STAR actual and expected growth. Then we conducted a multiple regression analysis with data about engagement with GraphoLearn included as predictors. Additional predictors were extracted from GraphoLearn performance at study onset to assess children’s letter-sound knowledge, rime awareness, and word recognition.

Results

The difference between actual average reading growth and expected growth in a regular school year was not statistically significant. This suggests that children in our sample seem to be gaining reading skills as expected in a regular school year. Our multiple linear regression model (which accounted for R2 = 48% of reading growth) showed that older children, with higher baseline GraphoLearn word recognition, who played more units in a fixed number of days, made significantly more early literacy progress.

Discussion

While lacking a control group, our preliminary results suggest that an EdTech program such as GraphoLearn may be a useful reading instructional tool during school shutdowns. In addition, our results suggest that practice with GraphoLearn was more effective and efficient when foundational instruction was already in place.
Influence of emotion on precision grip force control: A comparison of pleasant and neutral emotion
Authors: Bunno Yoshibumi , Onigata Chieko
Published: December 2022

Objective

The present study aimed to investigate the impact of emotion on force steadiness of isometric precision pinch grip that is not direction-specific.

Methods

Thirty-two healthy volunteer subjects participated in the present study. Subjects were divided into two experimental groups: pleasant image group and neutral image group. The isometric precision pinch grip task was performed for three times. Specifically, the first task was performed before pleasant or neutral picture viewing, the second task was performed immediately after picture viewing, further the third task was performed 30 seconds after the second task. During the isometric precision pinch grip task, participants were asked to exert pinch grip force at 10% of maximal voluntary contraction with visual feedback. The coefficient of variation of force production and normalized root mean square value of electromyography activity were calculated.

Results

After pleasant picture viewing, coefficient of variation of pinch force production and normalized root mean square value of electromyography was decreased. While, in the neutral image condition, theses variables were not altered. More important, compared to the neutral image condition, pleasant emotion led to lower coefficient of variation of pinch grip force production.

Conclusion

These findings indicate that pleasant emotion improves force control of isometric precision pinch grip. Therefore, in clinical settings, the emotional state of patients may affect the effectiveness of rehabilitation and should be taken into consideration.
From transcendental egology to orientation theory: Toward a mereological foundation for the different senses of the “self” in conscious experience
Authors: Guardiola Joan González
Published: December 2022

In the present work, we aim to make a contribution to the origins of the notion of “minimum self” in Husserl's phenomenology. Starting from the difference between the philosophy of the subject and the philosophy of the self, the aim of this research is to show that the Cartesian association between both philosophies would not exactly correspond to the conception of the self, as we find it in Edmund Husserl's works. With this, we intend to nuance Heidegger's accusation of Husserl's “Cartesianism,” At the same time, we show how a detailed analysis of the “senses of the self” in Husserl's phenomenology allows extracting the notion of “minimal self” as it has been introduced in the current and lively debate between psychiatry and phenomenology. In our research, we also show that in order to move the theory of the transcendental ego toward the theory of the orientation of the life of consciousness, it is necessary to consider the foundation of the concepts of ego in the technical vocabulary of the formal mereology of the Husserl's third “Logical Investigation.”
Digitalization and digital transformation in higher education: A bibliometric analysis
Authors: Díaz-García Vicente , Montero-Navarro Antonio , Rodríguez-Sánchez José-Luis , Gallego-Losada Rocío
Published: December 2022

The new paradigms that are emerging because of technological and social advances derived from the massive use of information and communication technologies (ICTs) are generating a transformative process that is modifying all economic sectors, and education is no exception. Higher Education Institutions (HEIs) are carrying out such transformation, reacting to the need of adaptation to this new reality, experiencing a complete cultural change that is challenging the attitudes, actions and values shared by the members and stakeholders of these organizations. In order to analyze the scientific literature about this topic, a bibliometric analysis has been carried out covering the period 1900–2021, considering a sample of 469 articles included in the Web of Science (WoS) database. The results show the multidisciplinary nature of the topic, including articles published in different areas, as well as its close link with aspects such as innovation, governance and agile methodologies. Finally, this study highlights the main lines of research that could attract more attention in the immediate future.
PrEP stigma among current and non-current PrEP users in Thailand: A comparison between hospital and key population-led health service settings
Authors: Chautrakarn Sineenart , Rayanakorn Ajaree , Intawong Kannikar , Chariyalertsak Chonlisa , Khemngern Porntip , Stonington Scott , Chariyalertsak Suwat
Published: December 2022

Background

Pre-exposure prophylaxis (PrEP) has demonstrated effectiveness in high-risk populations. PrEP service in Thailand became free of charge under the Universal Health Coverage (UHC) in 2021. The National Health Security Office launched a pilot project in 2020 to ensure sustainable service delivery, and the national monitoring and evaluation (M&E) framework was adopted to evaluate early phase implementation. We carried out a cross-sectional survey as part of the M&E process to investigate PrEP stigma among current and non-current PrEP users from both hospital and Key Population Led Health Services (KPLHS) settings in Thailand.

Methods

Between August and October 2020, an online cross-sectional survey was conducted. A link for a self-administered questionnaire was distributed to all active PrEP centers and PrEP clients were then recruited by PrEP providers. Descriptive and univariate analysis using Chi-square were applied in the analyses. Attitudes toward PrEP were ranked from the most negative to the most positive. The negative attitude can be interpreted as PrEP stigma.

Results

This study included 513 PrEP clients (355 from hospitals and 158 from KPLHS). In both settings, respondents' attitudes toward PrEP were generally positive, but some potential stigma was observed. 31.8% of hospital PrEP clients and 9.5% of KPLHS clients agreed that PrEP users should keep their pills hidden from others. Almost half (44.5%) of hospital clients and 18.4% of KPLHS clients agreed that PrEP users are often viewed negatively by society. More than 20% of hospital clients and 12% of KPLHS agreed that PrEP users frequently experience difficulties when their partner/lover/family find out that he or she is on PrEP. Respondents from the hospitals had slightly higher PrEP stigma than those from KPLHS.

Conclusions

According to our findings, at the policy level, the campaign to provide PrEP education to all groups of people should be continued in order to promote a positive view of PrEP and reduce PrEP-related stigma among the general population, which is critical for successful PrEP implementation.
Associations between air pollution and multimorbidity in the UK Biobank: A cross-sectional study
Authors: Ronaldson Amy , Arias de la Torre Jorge , Ashworth Mark , Hansell Anna L. , Hotopf Matthew , Mudway Ian , Stewart Rob , Dregan Alex , Bakolis Ioannis
Published: December 2022

Background

Long-term exposure to air pollution concentrations is known to be adversely associated with a broad range of single non-communicable diseases, but its role in multimorbidity has not been investigated in the UK. We aimed to assess associations between long-term air pollution exposure and multimorbidity status, severity, and patterns using the UK Biobank cohort.

Methods

Multimorbidity status was calculated based on 41 physical and mental conditions. We assessed cross-sectional associations between annual modeled particulate matter (PM)2.5, PMcoarse, PM10, and nitrogen dioxide (NO2) concentrations (μg/m3–modeled to residential address) and multimorbidity status at the baseline assessment (2006–2010) in 364,144 people (mean age: 52.2 ± 8.1 years, 52.6% female). Air pollutants were categorized into quartiles to assess dose-response associations. Among those with multimorbidity (≥2 conditions; n = 156,395) we assessed associations between air pollutant exposure levels and multimorbidity severity and multimorbidity patterns, which were identified using exploratory factor analysis. Associations were explored using generalized linear models adjusted for sociodemographic, behavioral, and environmental indicators.

Results

Higher exposures to PM2.5, and NO2 were associated with multimorbidity status in a dose-dependent manner. These associations were strongest when we compared the highest air pollution quartile (quartile 4: Q4) with the lowest quartile (Q1) [PM2.5: adjusted odds ratio (adjOR) = 1.21 (95% CI = 1.18, 1.24); NO2: adjOR = 1.19 (95 % CI = 1.16, 1.23)]. We also observed dose-response associations between air pollutant exposures and multimorbidity severity scores. We identified 11 multimorbidity patterns. Air pollution was associated with several multimorbidity patterns with strongest associations (Q4 vs. Q1) observed for neurological (stroke, epilepsy, alcohol/substance dependency) [PM2.5: adjOR = 1.31 (95% CI = 1.14, 1.51); NO2: adjOR = 1.33 (95% CI = 1.11, 1.60)] and respiratory patterns (COPD, asthma) [PM2.5: adjOR = 1.24 (95% CI = 1.16, 1.33); NO2: adjOR = 1.26 (95% CI = 1.15, 1.38)].

Conclusions

This cross-sectional study provides evidence that exposure to air pollution might be associated with having multimorbid, multi-organ conditions. Longitudinal studies are needed to further explore these associations.
How has the COVID-19 pandemic affected young people?—Mapping knowledge structure and research framework by scientometric analysis
Authors: Li Xiangfei , Yu Jiahui
Published: December 2022

Since the outbreak of COVID-19, there has been a large body of literature focusing on the relationship between the COVID-19 pandemic and young people. The purpose of this study is to explore the current research status and the specific mechanism of COVID-19's effects on young people based on related literature. This paper mainly used VOS viewer and CiteSpace software to conduct a scientometric analysis of 5,077 publications retrieved from the Web of Science database. The results show that the main contributors to the field were mainly from North America and Europe, and the trend of research focus was from shallow to deep. The five main research areas in the field were summarized by keyword clustering analysis as follows: lifestyle changes due to lockdown; changes in stress and emotions; psychological illness and trauma; risk perception and practice toward the epidemic; interventions and social support. Finally, they were linked by four pathways to form a framework that integrates the relationships between the five domains and between elements within each of them, revealing the mechanism of COVID-19's effect on young people. In addition, less studied but promising elements are also presented in the framework, such as research on special groups (disadvantaged socioeconomic groups and sexual minority youth) and extreme suicidal tendencies that deserve our further attention.
Cardiovascular consequences of aircraft noise exposure
Authors: Ba̧czalska Justyna , Wojciechowska Wiktoria , Rojek Marta , Hahad Omar , Daiber Andreas , Münzel Thomas , Rajzer Marek
Published: December 2022

The results from epidemiological studies suggest that environmental noise including aircraft, railway, road traffic, wind turbine, and leisure-related noise is a growing public health concern. According to the WHO, at least 100 million people in the European Union are affected by traffic noise levels above the WHO-recommended thresholds. Environmental noise can adversely affect physical and mental health, as well as wellbeing. Chronic low-level noise exposure typical for most environmental sources is associated with psychophysiological stress causing non-auditory or indirect noise effects leading ultimately to cardiovascular diseases. Among all environmental noise sources, aircraft noise is considered the most annoying, and its leading mechanism of action is autonomic system activation such as increases in heart rate and blood pressure. Previously, we observed that long-term exposure to aircraft noise was associated with increased diastolic blood pressure, arterial stiffness (as assessed by pulse wave velocity), and impaired left ventricular diastolic function. All mentioned above effects are early, subclinical, and potentially reversible changes which preceded late noise effects in the cardiovascular system, that is, established cardiovascular diseases such as myocardial infarction, stroke, and heart failure. However, even a short-term reduction in aircraft noise exposure as observed during the COVID-19 lockdown may reverse these negative effects on arterial stiffness and blood pressure and may decrease the prevalence of insomnia. In this review, we aimed to critically discuss our obtained results considering recent studies on the influence of aircraft noise (and other traffic noises) on cardiovascular diseases in the context of the WHO Environmental Noise Guidelines for the European Region.
Influenza incidence and air pollution: Findings from a four-year surveillance study of prefecture-level cities in China
Authors: Zhang Yu , Wang Shijun , Feng Zhangxian , Song Yang
Published: December 2022

Background

Influenza is a serious public health problem, and its prevalence and spread show significant spatiotemporal characteristics. Previous studies have found that air pollutants are linked to an increased risk of influenza. However, the mechanism of influence and the degree of their association have not been determined. This study aimed to determine the influence of the air environment on the spatiotemporal distribution of influenza.

Methods

The kernel density estimation and Getis-Ord Gi* statistic were used to analyze the spatial distribution of the influenza incidence and air pollutants in China. A simple analysis of the correlation between influenza and air pollutants was performed using Spearman's correlation coefficients. A linear regression analysis was performed to examine changes in the influenza incidence in response to air pollutants. The sensitivity of the influenza incidence to changes in air pollutants was evaluated by performing a gray correlation analysis. Lastly, the entropy weight method was used to calculate the weight coefficient of each method and thus the comprehensive sensitivity of influenza incidence to six pollution elements.

Results

The results of the sensitivity analysis using Spearman's correlation coefficients showed the following ranking of the contributions of the air pollutants to the influenza incidence in descending order: SO2 >NO2 >CO> PM2.5 >O3 >PM10. The sensitivity results obtained from the linear regression analysis revealed the following ranking: CO>NO2 >SO2 >O3 >PM2.5 >PM10. Lastly, the sensitivity results obtained from the gray correlation analysis showed the following ranking: NO2 >CO>PM10 >PM2.5 >SO2 >O3. According to the sensitivity score, the study area can be divided into hypersensitive, medium-sensitive, and low-sensitive areas.

Conclusion

The influenza incidence showed a strong spatial correlation and associated sensitivity to changes in concentrations of air pollutants. Hypersensitive areas were mainly located in the southeastern part of northeastern China, the coastal areas of the Yellow River Basin, the Beijing-Tianjin-Hebei region and surrounding areas, and the Yangtze River Delta. The influenza incidence was most sensitive to CO, NO2, and SO2, with the occurrence of influenza being most likely in areas with elevated concentrations of these three pollutants. Therefore, the formulation of targeted influenza prevention and control strategies tailored for hypersensitive, medium-sensitive, low-sensitive, and insensitive areas are urgently needed.
Dietary factors and the risk of lung cancer by epidermal growth factor receptor mutation status and histological subtypes
Authors: Yin Xin , Lai Gillianne Geet Yi , Seow Adeline , Tan Daniel Shao Weng , Lim Darren Wan-Teck , Seow Wei Jie
Published: December 2022

Background

Previous studies have reported differential associations of certain dietary factors such as soy consumption by epidermal growth factor receptor mutant (EGFR +) subtype of non-small cell lung cancer (NSCLC). However, whether the other dietary factors including meat, fruits, and vegetables have differential risks on different histological and molecular subtypes of lung cancer remains unclear. Therefore, we conducted a case-control study to evaluate these associations.

Methods

A total of 3,170 cases and 4,238 controls from three different studies (Genes and Environment in Lung Cancer Study, Lung Cancer Consortium Singapore Study, and Multi-ethnic Cohort Study) were included. Information on demographics, lifestyle, and dietary consumption was obtained using questionnaires. Diet was assessed by using the number of standard servings of each item consumed per week. Multivariable logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the association between meat, vegetables, and fruits consumption with lung cancer risk after adjusting for potential confounders.

Results

We identified a significant inverse association between higher consumption of fruits and the risk of lung cancer (2nd tertile: OR = 0.54, 95%CI = 0.46–0.65; 3rd tertile: OR = 0.77, 95%CI = 0.65–0.91), compared with the lower (1st tertile) consumption of fruits. Higher vegetable consumption was significantly associated with a lower risk of EGFR + lung cancer (OR = 0.69, 95% CI = 0.54–0.88), however, this association was not significant among EGFR wild-type (−) lung cancer. Conversely, higher consumption of total meat (OR = 2.10, 95%CI = 1.58–2.79) was significantly associated with higher lung cancer risk, as compared with the lower consumption group.

Conclusions

Differential associations between vegetable consumption with EGFR mutation status in NSCLC were found. Further prospective studies are warranted to assess this association and elucidate the biological mechanisms.