bluuebunny/crossref_metadata_2025 huggingface dataset:
New Insights into the Pathogenesis of Giant Cell Arteritis: Mechanisms Involved in Maintaining Vascular Inflammation
Authors: Greigert Hélène , Genet Coraline , Ramon André , Bonnotte Bernard , Samson Maxime
Published: May 2022

The giant cell arteritis (GCA) pathophysiology is complex and multifactorial, involving a predisposing genetic background, the role of immune aging and the activation of vascular dendritic cells by an unknown trigger. Once activated, dendritic cells recruit CD4 T cells and induce their activation, proliferation and polarization into Th1 and Th17, which produce interferon-gamma (IFN-γ) and interleukin-17 (IL-17), respectively. IFN-γ triggers the production of chemokines by vascular smooth muscle cells, which leads to the recruitment of additional CD4 and CD8 T cells and also monocytes that differentiate into macrophages. Recent data have shown that IL-17, IFN-γ and GM-CSF induce the differentiation of macrophage subpopulations, which play a role in the destruction of the arterial wall, in neoangiogenesis or intimal hyperplasia. Under the influence of different mediators, mainly endothelin-1 and PDGF, vascular smooth muscle cells migrate to the intima, proliferate and change their phenotype to become myofibroblasts that further proliferate and produce extracellular matrix proteins, increasing the vascular stenosis. In addition, several defects in the immune regulatory mechanisms probably contribute to chronic vascular inflammation in GCA: a defect in the PD-1/PD-L1 pathway, a quantitative and qualitative Treg deficiency, the implication of resident cells, the role of GM-CSF and IL-6, the implication of the NOTCH pathway and the role of mucosal-associated invariant T cells and tissue-resident memory T cells.
Curcumin: Useful add-on for Rheumatic Diseases?
Authors: Tomaras Stylianos , Keyßer Gernot , Feist Eugen
Published: May 2022

Plant-derived nutraceuticals are proposed as new key instruments to represent a profound “back to basics” shift in medical treatment. Data accumulated over the past ten years suggest that curcumin, the major active compound of the turmeric plant, has anti-inflammatory properties. It has yet to be determined whether the anti-inflammatory profile of curcumin is potent enough to justify the application of this substance as a nutritional supplement for patients with rheumatic diseases. To address this question, the most relevant in vitro studies that investigate the mechanism of action of curcumin were reviewed in this article. In addition, a total of 18 animal and human trials were evaluated. The pleiotropic, anti-inflammatory and immunomodulatory effects of curcumin were observed in animal studies. In addition, human trials demonstrated promising findings. In these studies, curcumin was able to reduce the expression of proinflammatory cytokines, lower the level of the C-reactive protein and improve clinical parameters. A limiting factor of the application of curcumin is the inconsistent bioavailability of the substance. Therefore, new formulations have been developed to improve the pharmacodynamic profile of curcumin. The future acceptance of the substance is dependent on new controlled clinical trials with a standardised formulation of curcumin administered as well as standard of care.
Circular RNAs Acting as miRNAs’ Sponges and Their Roles in Stem Cells
Authors: Xiao Juan , Joseph Shija , Xia Mengwei , Teng Feng , Chen Xuejiao , Huang Rufeng , Zhai Lihong , Deng Wenbin
Published: May 2022

Circular RNAs (circRNAs), a novel type of endogenous RNAs, have become a subject of intensive research. It has been found that circRNAs are important players in cell differentiation and tissue homeostasis, as well as disease development. Moreover, the expression of circRNAs is usually not correlated with their parental gene expression, indicating that they are not only a steady-state by-product of mRNA splicing but a product of variable splicing under novel regulation. Sequence conservation analysis has also demonstrated that circRNAs have important non-coding functions. CircRNAs exist as a covalently closed loop form in mammalian cells, where they regulate cellular transcription and translation processes. CircRNAs are built from pre-messenger RNAs, and their biogenesis involves back-splicing, which is catalyzed by spliceosomes. The splicing reaction gives rise to three different types of intronic, exotic and exon–intron circular RNAs. Due to higher nuclease stability and longer half lives in cells, circRNAs are more stable than linear RNAs and have enormous clinical advantage for use as diagnostic and therapeutic biomarkers for disease. In recent years, it has been reported that circRNAs in stem cells play a crucial role in stem cell function. In this article, we reviewed the general feature of circRNAs and the distinct roles of circRNAs in stem cell biology, including regulation of stem cell self-renewal and differentiation. CircRNAs have shown unique expression profiles during differentiation of stem cells and could serve as promising biomarkers of these cells. As circRNAs play pivotal roles in stem cell regulation as well as the development and progression of various diseases, we also discuss opportunities and challenges of circRNA-based treatment strategies in future effective therapies for promising clinical applications.
Searching for New Genetic Biomarkers of Axial Spondyloarthritis
Authors: Bugaj Bartosz , Wielińska Joanna , Bogunia-Kubik Katarzyna , Świerkot Jerzy
Published: May 2022

Background: Axial spondyloarthritis (axSpA) is a chronic inflammatory condition of the spine. In addition to musculoskeletal symptoms, there are also extra-articular manifestations. The aim of this study was to search for new biomarkers associated with the clinical presentation and treatment response in axSpA patients. Methods: In this study, 106 axSpA patients and 110 healthy controls were enrolled. Six single-nucleotide polymorphisms (SNPs) were selected for genotyping: ERAP1 rs2287987, ERAP2 rs2549782, TNF rs1800629, TNFRSF1A rs767455, TNFRSF1B rs1061622, and FCGR2A rs1801274. Participants were examined at baseline and after 12 and 24 weeks of anti-TNF therapy. Results: SNPs associated with high axSpA initial activity were TNFRSF1A rs767455 and TNFRSF1B rs1061622 (p < 0.008). The ERAP1 rs2287987 AA genotype was more frequently observed in patients with enthesitis (AA vs. G+, p = 0.049), while the TNFRSF1B rs1061622 GG genotype was more common in participants with uveitis (GG vs. TT, p = 0.042). Potential in predicting anti-TNF treatment response was demonstrated by ERAP1 rs2287987, ERAP2 rs2549782, TNFRSF1B rs1061622, and FCGR2A rs1801274. Conclusions: SNPs can be used to identify patients at risk of severe disease to initiate treatment earlier. Genetic testing will allow clinicians to choose the right drug for the patient.
Natural Feed Supplements Improve Growth, Non-Specific Immune Responses and Resistance against Vibrio alginolyticus in Lates calcarifer
Authors: Liao Zhen-Hao , Huang Huai-Ting , Lin Yu-Ru , Chen Bo-Ying , Lee Yi-Fan , Lin Yuan-Heng , Chuang Chieh-Yuan , Nan Fan-Hua
Published: May 2022

This study aimed to investigate the effects of dietary natural feed supplement on the growth performance, non-specific responses, and disease resistance in Lates calcarifer. Three commercial products (A, B, and C) containing a basal probiotic mixture were tested. Product A contained a basal mixture of Paenibacillus sp., Bacillus subtilis, Bacillus amyloliquefaciens, and Lactobacillus rhamnosus (107 cfu/g for each probiotic); product B contained additional Lactobacillus plantarum (1010 cfu/g); and product C contained additional soybean peptides (500 g/kg) and garlic powder (1 g/kg). Each product was supplemented into subject diets at dosages of 1 or 2 g/kg (designated as the A1, A2, B1, B2, C1, and C2 groups, respectively). Following an eight-week trial, growth parameters (specific growth rate and feed conversion ratio), non-specific immune responses (O2− production, phagocytic rate, and phagocytic index), and the results of a challenge test against Vibrio alginolyticus were evaluated. The results show that all probiotic supplement groups exhibited an improvement in growth performance compared to the control group (non-probiotic diet). In terms of non-specific immunity parameters, a significant improvement in O2− production was found in the C2 group, whereas significant improvements in phagocytic activity were found in all the B and C groups. The C2 group displayed optimal O2− production, phagocytic rate, and phagocytic index results. For the challenge test, the C groups showed higher Vibrio resistance than the other experimental groups and the control group. These results suggest that product C, given at dosages of 2 g/kg, may serve as a growth-promoting and immunostimulatory additive for the cultivation of Asian seabass.
Critique on Ecological Methodologies Used in Water Quality Studies and Coastal Management: A Review
Authors: Karydis Michael
Published: May 2022

The subject of ecology is the understanding of the relations among living organisms and their interactions with the abiotic environment. The need to quantify ecological phenomena requires the development of mathematical tools, including ecological indices, statistical procedures and simulation models. Some of these tools have been found to be convenient by many scientists and policy makers in related scientific disciplines to express marine pollution levels, marine water quality and future trends; they have, therefore, been adopted in coastal management methodologies and practices. In the present work, a number of ecological tools are reviewed regarding their relevance to supporting water quality studies, as well as their suitability to be included in the toolkit of coastal management practices. Their problems and weaknesses, together with the science–policy misconnection, are discussed.
Evaluation of Pharmacogenetics of Drug-Metabolizing Enzymes and Drug Efflux Transporter in Renal Transplants Receiving Immunosuppressants
Authors: Sridharan Kannan , Shah Shamik , Jassim Anfal , Hammad Mona , Ebrahim Al Gadhban Johaina , Al Segai Ola
Published: May 2022

Cytochrome P450 (CYP) enzymes, such as CYP3A4, and CYP3A5, P450 oxidoreductase (POR), peroxisome proliferator activated receptor alpha (PPAR-alpha), and drug transporter (ABCB1) were observed to influence concentrations of immunosuppressants (cyclosporine, everolimus, sirolimus, and tacrolimus) and outcomes in renal transplants. We carried out the present study to evaluate the prevalence and impact of these single nucleotide polymorphisms (SNPs) in adult renal transplants. SNPs were evaluated using commercial TaqMan® assays. Serum drug concentrations were estimated using immunoassays. One hundred and forty-six patients were recruited. SNPs in CYP3A5*3 were significantly associated with greater dose-adjusted cyclosporine and tacrolimus concentrations. SNPs in POR*28 were observed with significantly lower dose-adjusted concentrations, particularly with cyclosporine and tacrolimus. ABCB1 homozygous polymorphisms were observed with significantly lower time spent in the therapeutic range with cyclosporine and everolimus/sirolimus. Cyclosporine was observed in a significantly greater proportion of patients with elevated GGT, and SNPs in PPAR-alpha were significantly associated with an increased risk of this adverse event. Hypertriglyceridemia with everolimus was significantly associated with POR*28 polymorphisms. There is a need to validate the influence of these SNPs in a prospective study and develop an algorithm predicting the achievement of target concentrations.
Tailored Treatment Options for Cerebral Cavernous Malformations
Authors: Hoffman Jessa E. , Wittenberg Blake , Morel Brent , Folzenlogen Zach , Case David , Roark Christopher , Youssef Samy , Seinfeld Joshua
Published: May 2022

The diagnosis and treatment of cerebral cavernous malformations (CCMs), or cavernomas, continues to evolve as more data and treatment modalities become available. Intervention is necessary when a lesion causes symptomatic neurologic deficits, seizures, or has high risk of continued hemorrhage. Future medical treatment directions may specifically target the pathogenesis of these lesions. This review highlights the importance of individualized treatment plans based on specific CCM characteristics.
Three-Dimensional Evaluation of Isodose Radiation Volumes in Cases of Severe Mandibular Osteoradionecrosis for the Prediction of Recurrence after Segmental Resection
Authors: Glas Haye H. , Kraeima Joep , Tribius Silke , Leusink Frank K. J. , Rendenbach Carsten , Heiland Max , Stromberger Carmen , Rashad Ashkan , Fuller Clifton D. , Mohamed Abdallah S. R. , Lai Stephen Y. , Witjes Max J. H.
Published: May 2022

Background: Pre-operative margin planning for the segmental resection of affected bone in mandibular osteoradionecrosis (ORN) is difficult. The aim of this study was to identify a possible relation between the received RT dose, exposed bone volume and the progression of ORN after segmental mandibular resection. Method: Patients diagnosed with grade 3-4 ORN for which a segmental resection was performed were included in the study. Three-dimensional reconstructions of RT isodose volumes were fused with postoperative imaging. The primary outcome was the recurrence of ORN after segmental resection. Subsequently, RT exposed mandibular bone volumes were calculated and the location of the bone cuts relative to the isodose volumes were assessed. Results: Five out of thirty-three patients developed recurrent ORN after segmental mandibular resection. All cases with recurrent ORN were resected inside an isodose volume of ≥56 Gy. The absolute mandibular volume radiated with 56 Gy was significantly smaller in the recurrent group (10.9 mL vs. 30.7 mL, p = 0.006), as was the proportion of the mandible radiated with 56 Gy (23% vs. 45%, p = 0.013). Conclusion: The volume of radiated bone was not predictive for risk of progression. The finding that recurrent ORN occurred with bone resection margins within the 56 Gy isodose volume suggests that this could serve as a starting point for the pre-operative planning of reducing the risk of ORN recurrence.
Studies in Rats of Combined Muscle and Liver Perfusion and of Muscle Extract Indicate That Contractions Release a Muscle Hormone Directly Enhancing Hepatic Glycogenolysis
Authors: Han Xiao X. , Holst Jens J. , Galbo Henrik
Published: May 2022

Background: Established neuroendocrine signals do not sufficiently account for the exercise-induced increase in glucose production. Using an innovative, yet classical cross-circulation procedure, we studied whether contracting muscle produces a factor that directly stimulates hepatic glycogenolysis. Methods: Isolated rat hindquarters were perfused in series with isolated livers. Results: Stimulation of the sciatic nerve of one or both legs resulted in an increase in force, which rapidly waned. During one-legged contractions, hepatic glucose production increased initially (from −0.9 ± 0.5 (mean ± SE) to 3.3 ± 0.7 µmol/min, p < 0.05). The peak did not differ significantly from that seen after 20 nM of epinephrine (5.1 ± 1.2 µmol/min, p > 0.05). In response to two-legged contractions, the increase in hepatic glucose production (to 5.4 ± 1.3 µmol/min) was higher (p < 0.05) and lasted longer than that seen during one-legged contractions. During contractions, peak hepatic glucose output exceeded concomitant hepatic lactate uptake (p < 0.05), and glucose output decreased to basal levels, while lactate uptake rose to a plateau. Furthermore, in separate experiments an increase in lactate supply to isolated perfused livers increased lactate uptake, but not glucose output. In intact rats, intra-arterial injection of extract made from mixed leg muscle elicited a prolonged increase (p < 0.05) in plasma glucose concentration (from 5.2 ± 0.1 mM to 8.3 ± 1.5 mM). In perfused livers, muscle extract increased glucose output dose dependently. Fractionation by chromatography of the extract showed that the active substance had a MW below 2000. Conclusion: This study provides evidence that contracting skeletal muscle may produce a hormone with a MW below 2000, which enhances hepatic glycogenolysis according to energy needs. Further chemical characterization is warranted.
Constipation Symptoms in Multiple System Atrophy Using Rome Criteria and Their Impact on Personalized Medicine
Authors: Mishima Takayasu , Fujioka Shinsuke , Kawazoe Miki , Inoue Kotoe , Arima Hisatomi , Tsuboi Yoshio
Published: May 2022

Constipation is one of the most common non-motor symptoms in multiple system atrophy (MSA); however, it has not been evaluated according to the standard diagnostic criteria for constipation in patients with MSA. We evaluated the characteristics of constipation in patients with MSA by using Rome criteria (Rome III), which has been validated and the widely used for gastrointestinal disorders. Fifty-one patients with MSA (29 female) were enrolled in the study. Based on the Rome III criteria, constipation was diagnosed in 29 patients (56.9%); irritable bowel syndrome was not detected. Thirty-seven patients (72.5%) were aware of their constipation. The most common constipation symptom was the sensation of anorectal obstruction (68.6%). Patients’ self-awareness of constipation was most strongly correlated to the sensation of incomplete evacuation (odds ratio: 7.377, 95% confidence interval: 1.402–38.817). The number of constipation-related symptoms was correlated with the total levodopa equivalent dose (p < 0.05). Rome criteria, which can detect various constipation symptoms, are useful for evaluating constipation in MSA, and these findings may greatly impact personalized medicine.
Associations between Choriocapillaris Flow on Optical Coherence Tomography Angiography and Cardiovascular Risk Profiles of Patients with Acute Myocardial Infarction
Authors: Kim Dae Sung , Kim Byung Sik , Cho Heeyoon , Shin Jeong-Hun , Shin Yong Un
Published: May 2022

We evaluated the association between macular perfusion assessed via optical coherence tomography angiography (OCTA) and the cardiovascular risk profiles of patients with acute myocardial infarction (AMI). Patients with AMI who underwent comprehensive ophthalmological examinations and retinal OCTA were evaluated retrospectively. Retinal OCTA was performed for each patient within 3 days of admission. Quantitative analyses of the vessel density (VD) of the superficial capillary plexus (SCP) layers, deep capillary plexus layers (DCP), and choriocapillaris were performed after image processing. The 10-year risk of atherosclerotic cardiovascular disease (ASCVD), Global Registry of Acute Coronary Events (GRACE) score, reduction of atherothrombosis for continued health (REACH) score, and thrombolysis in myocardial infarction (TIMI) risk score were assessed. Sixty patients were included. VD in SCP and DCP was not associated with a 10-year ASCVD risk; however, choriocapillaris flow void features were significantly correlated with the 10-year ASCVD risk: decreased count, increased average size, and increased signal void area were correlated with increased 10-year ASCVD risk, GRACE score, REACH score, and TIMI risk score. In the multivariate analysis, a 10-year ASCVD risk (adjusted odds ratio [OR], 1.04; 95% confidence interval [CI], 1.01–1.08) and brain natriuretic peptide (adjusted OR, 1.00; 95% CI, 1.00–1.01) were significantly associated with the highest tertile of the average size of the choriocapillaris. Choriocapillaris flow void features measured using OCTA were associated with cardiovascular risk profiles in patients with AMI. OCTA may be used as an indicator of cardiovascular risk profiles and could improve cardiovascular risk assessments.
Diffuse Intrinsic Pontine Glioma: Molecular Landscape, Evolving Treatment Strategies and Emerging Clinical Trials
Authors: Damodharan Sudarshawn , Lara-Velazquez Montserrat , Williamsen Brooke Carmen , Helgager Jeffrey , Dey Mahua
Published: May 2022

Diffuse intrinsic pontine glioma (DIPG) is a type of intrinsic brainstem glial tumor that occurs primarily in the pediatric population. DIPG is initially diagnosed based on clinical symptoms and the characteristic location on imaging. Histologically, these tumors are characterized by a heterogenous population of cells with multiple genetic mutations and high infiltrative capacity. The most common mutation seen in this group is a lysine to methionine point mutation seen at position 27 (K27M) within histone 3 (H3). Tumors with the H3 K27M mutation, are considered grade 4 and are now categorized within the H3 K27-altered diffuse midline glioma category by World Health Organization classification. Due to its critical location and aggressive nature, DIPG is resistant to the most eradicative treatment and is universally fatal; however, modern advances in the surgical techniques resulting in safe biopsy of the lesion have significantly improved our understanding of this disease at the molecular level. Genomic analysis has shown several mutations that play a role in the pathophysiology of the disease and can be targeted therapeutically. In this review, we will elaborate on DIPG from general aspects and the evolving molecular landscape. We will also review innovative therapeutic options that have been trialed along with new promising treatments on the horizon.
Bank Risk-Taking and Legal Origin: What Do We Know about Dual Banking Economies?
Authors: Ali Mohsin , Alam Nafis , Khattak Mudeer Ahmed , Azmi Wajahat
Published: May 2022

This paper investigates the relationship between legal origin and banks’ risk-taking behavior. We employ GMM to study a sample of both Islamic and conventional banks from 14 dual banking economies from 2005–2018. Our findings can be summarized as follows: (a) bank risk-taking and legal origin are negatively related in our sample countries, (b) Islamic banks are more stable in English law (common) countries, and (c) bank regulations have a differential effect on Islamic and the conventional banks. Our overall findings align with the dark side of the legal framework, indicating a robust legal framework to encourage bank risk-taking. The results have several implications for shareholders, regulators, and other key stakeholders.
Impact of Liquidity Coverage Ratio on Performance of Select Indian Banks
Authors: Sidhu Anureet Virk , Rastogi Shailesh , Gupte Rajani , Bhimavarapu Venkata Mrudula
Published: May 2022

The post-crisis liquidity framework improves banking stability by imposing stricter liquidity requirements. However, consistent bank performance continues to be an essential factor in achieving this goal. This study examines the impact of the liquidity coverage ratio (LCR) on the profitability and non-performing assets (NPAs) of Indian banks using annual data from 2010 to 2019. By applying the dynamic panel data regression technique, we found that compliance with the minimum level of the LCR reduces the net interest margins (NIMs) of banks due to a narrower interest spread, thereby impacting banks profitability. Moreover, the NPAs of the banks tend to grow with an increase in LCR. The study’s findings have far-reaching implications for policymakers. Indian policymakers/regulators need to understand the strategies used by banks to meet liquidity standards and, if necessary, revisit the policy framework to achieve better compliance results. The study’s framework establishes a foundation that can be used for conducting similar research in other complex geographies such as India.
Increased Levels of Beta-Endorphin and Noradrenaline after a Brief High-Impact Multidimensional Rehabilitation Program in Multiple Sclerosis
Authors: d’Arma Alessia , Saresella Marina , Rossi Valentina , Marventano Ivana , Piancone Federica , La Rosa Francesca , Clerici Mario , Mendozzi Laura
Published: May 2022

Finding new solutions for the management of multiple sclerosis (MS) is crucial: further research is needed to study the effect of non-pharmacological interventions on the symptoms and the course of the disease, especially on lifestyle. Benefits from a proper lifestyle are evident not only on a clinical level but also on immune and neuro-endocrine systems. A brief high-impact multidimensional rehabilitation program (b-HIPE) was proposed for a sample of people with MS (pwMS) with a medium level of disease disability. We tested the change on clinical parameters and quality of life (QoL) after participation in B-HIPE. We furthermore decided to measure beta-endorphin and catecholamines concentrations pre- and post-participation in the b-HIPE program, due to the relationship between these hormones and the immune system in neurodegenerative diseases. Our results showed that after the b-HIPE program, an improvement of clinical parameters and QoL occurred. Moreover, we found higher levels of beta-endorphin and noradrenaline after participation in the program. These findings highlight the importance of implementing lifestyle interventions in the clinical management of MS. Furthermore, we hypothesize that the B-HIPE program increased beta-endorphin and noradrenaline levels, helping to reduce the inflammation related to MS disease.
Functional Properties of Cancer Epithelium and Stroma-Derived Exosomes in Head and Neck Squamous Cell Carcinoma
Authors: Li Yang , Gao Shengtao , Hu Qi , Wu Fanglong
Published: May 2022

Stroma–cancer cell crosstalk involves a complex signaling network that contributes to tumor progression, including carcinogenesis, angiogenesis, migration, invasion, and therapy resistance in cancers. Exosomes, as extracellular membranous nanovesicles released by almost all types of cells, including tumor cells and stromal cells, play a critical role in signal delivery and material communication, in which the characteristics of their parent cells are reflected. The tumor or stroma-derived exosomes mediate cell–cell communication in the tumor microenvironment by transporting DNA, RNA, proteins, lipids, and metabolites. Recent studies on head and neck squamous cell carcinoma (HNSCC) have demonstrated that tumor-derived exosomes support various tumor biological behaviors, whereas the functional roles of stroma-derived exosomes remain largely unknown. Although these exosomes are emerging as promising targets in early diagnosis, prognostic prediction, and pharmaceutical carriers for antitumor therapy, there are still multiple hurdles to be overcome before they can be used in clinical applications. Herein, we systematically summarize the promotive roles of the epithelium and stroma-derived exosomes in HNSCC and highlight the potential clinical applications of exosomes in the treatment of HNSCC.
The Effectiveness of Shoulder Mobility and Strength Programs in Competitive Water-Polo Players
Authors: López-Laval Isaac , Sitko Sebastian , Cantonero Jaime , Corbi Francisco , Cirer-Sastre Rafel
Published: May 2022

Background: Water-polo is the water sport with the highest incidence of injuries, with shoulder pain being the most common one. The understanding of risk factors and guidance on preventive measures is essential in this sport discipline. The aim of this study was to determine the effects of a specific 6-week training plan on range of motion (ROM) and joint strength levels in a group of professional water-polo players. Methods: Quasi-experimental study with a sample of 28 participants (age: 20.1 ± 2.5 years; height: 176.9 ± 6.2 cm; body mass: 74.6 ± 8.1 kg). Three study groups, which consisted of one control group and two experimental groups, were established. Two repeated measurements, pre and post intervention, were performed. During these measurements, ROM of the glenohumeral joint was analyzed both in external (ER) and internal (IR) rotation, as well as the maximal isometric strength. Conclusions: The application of a training program improved glenohumeral joint ROM. ER and IR evolve differently in both shoulders. ER improved only in the throwing arm only in the group undergoing intervention but for the non-dominant side, improvements were observed in both ER and IR, regardless of whether or not they had followed the intervention plan. No improvements were observed in either the isometric strength or contralateral asymmetries.
Revisiting DNA Sequence-Dependent Deformability in High-Resolution Structures: Effects of Flanking Base Pairs on Dinucleotide Morphology and Global Chain Configuration
Authors: Young Robert T. , Czapla Luke , Wefers Zoe O. , Cohen Benjamin M. , Olson Wilma K.
Published: May 2022

DNA carries more than the list of biochemical ingredients that drive the basic functions of living systems. The sequence of base pairs includes a multitude of structural and energetic signals, which determine the degree to which the long, threadlike molecule moves and how it responds to proteins and other molecules that control its processing and govern its packaging. The chemical composition of base pairs directs the spatial disposition and fluctuations of successive residues. The observed arrangements of these moieties in high-resolution protein–DNA crystal structures provide one of the best available estimates of the natural, sequence-dependent structure and deformability of the double-helical molecule. Here, we update the set of knowledge-based elastic potentials designed to describe the observed equilibrium structures and configurational fluctuations of the ten unique base-pair steps. The large number of currently available structures makes it possible to characterize the configurational preferences of the DNA base-pair steps within the context of their immediate neighbors, i.e., tetrameric context. Use of these knowledge-based potentials shows promise in accounting for known effects of sequence in long chain molecules, e.g., the degree of curvature reported in classic gel mobility studies and the recently reported sequence-dependent responses of supercoiled minicircles to nuclease cleavage.
Influence of Bionic Circular Groove Blade Surface on Wear Performance
Authors: Ma Longbiao , Gu Yunqing , Xia Ke , Mou Chengqi , Mou Jiegang , Wu Denghao , Yan Muhan
Published: May 2022

In order to improve the anti-wear performance of a double-vane self-priming centrifugal pump during two-phase flow transfer, the construction of a streamline groove structure at the outlet end of the suction side of the vane, based on the bionic principle, is proposed. Different pump characteristics are analysed to investigate the effect of different bionic groove spacing on the resistance to particle wear and the mechanism of improvement of the bionic grooves. The results show that the effect of the bionic blades on the hydraulic characteristics of the pump is within the allowable error (±1.4%). The circular groove structure with different spacing produces a difference in the pressure distribution on the blade. At the same particle concentration, with the increase in the groove spacing distance, the average wear of the blades first decreases and then increases; the average wear rate at the spacing of 7 mm is the smallest. At a particle concentration of 90 kg/m3, the average wear rate at a groove spacing of 7 mm is ~0.63 × 10−4 kg/s∙m2, and the wear area is mainly found in the middle of the blade. The reason why the bionic blade improves the anti-wear performance of the pump is due to the reverse vortex zone in the groove, which changes the particle trajectory and collision frequency. The bionic grooves with a diameter of 2 mm and a spacing of 7 mm significantly reduce the average wear rate of the pump at different particle concentrations, while maintaining hydraulic performance.
Corrosion Behavior of CrFeCoNiVx (x = 0.5 and 1) High-Entropy Alloys in 1M Sulfuric Acid and 1M Hydrochloric Acid Solutions
Authors: Tsau Chun-Huei , Chen Jo-Yi , Chien Tien-Yu
Published: May 2022

CrFeCoNiVx high-entropy alloys were prepared by arc-melting, and the microstructures and corrosion properties of these alloys were studied. The CrFeCoNiV0.5 alloy had a granular structure; the matrix was a face-centered cubic (FCC) structure, and the second phase was a σ phase with a tetragonal structure. The CrFeCoNiV alloy had a dendritic structure; the dendrites in this alloy showed an FCC phase, and the interdendrities had a eutectic structure of FCC and σ phases. Therefore, CrFeCoNiV was much harder than the CrFeCoNiV0.5 alloy due to the dendritic structures. The potentiodynamic polarization test and electrochemical impedance spectroscopy were used to evaluate the corrosion behavior of the CrFeCoNiVx high-entropy alloys in deaerated 1M sulfuric acid and 1M hydrochloric acid solutions. The results indicated that the CrFeCoNiV0.5 alloy had a better corrosion resistance because of the granular structure.
New n-p Junction Floating Gate to Enhance the Operation Performance of a Semiconductor Memory Device
Authors: Chen Yi-Yueh , Lin Su-Jien , Chang Shou-Yi
Published: May 2022

To lower the charge leakage of a floating gate device and improve the operation performance of memory devices toward a smaller structure size and a higher component capability, two new types of floating gates composed of pn-type polysilicon or np-type polysilicon were developed in this study. Their microstructure and elemental compositions were investigated, and the sheet resistance, threshold voltages and erasing voltages were measured. The experimental results and charge simulation indicated that, by forming an n-p junction in the floating gate, the sheet resistance was increased, and the charge leakage was reduced because of the formation of a carrier depletion zone at the junction interface serving as an intrinsic potential barrier. Additionally, the threshold voltage and erasing voltage of the np-type floating gate were elevated, suggesting that the performance of the floating gate in the operation of memory devices can be effectively improved without the application of new materials or changes to the physical structure.
Estimation of Nanoporous Au Young’s Modulus from Serial Block Face-SEM 3D-Characterisation
Authors: Brun Michele , Sogne Elisa , Falqui Andrea , Scaglione Federico , Rizzi Paola , Delogu Francesco , Pia Giorgio
Published: May 2022

Nanoporous Au has been subjected to serial block face-scanning electron microscopy (SBF-SEM) 3D-characterisation. Corresponding sections have been digitalized and used to evaluate the associated mechanical properties. Our investigation demonstrates that the sample is homogeneous and isotropic. The effective Young’s modulus estimated by an analytical multiscale approach agrees remarkably well with the values stated in the literature.
Nanofused Hierarchically Porous MIL-101(Cr) for Enhanced Methyl Orange Removal and Improved Catalytic Activity
Authors: Zou Minmin , Dong Ming , Luo Mingliang , Zhu Hexin , Zhao Tian
Published: May 2022

Hierarchically porous MIL-101(Cr) (H-MIL-101(Cr)) with meso/macro-pores was directly prepared via nanofusion progress by using butyric acid as a modulating agent. In the methyl orange (MO) adsorption experiments, H-MIL-101(Cr) showed a high adsorption capability of 369.8 mg g−1, which was 1.52-fold greater than that of pristine MIL-101(Cr) (P-MIL-101(Cr)). While in the oxidation reaction of indene and 1-dodecene tests, H-MIL-101(Cr) presented much higher catalytic efficiency, with turnover frequency (TOF) values of 0.7242 mmol g−1 min−1 and 0.1492 mmol g−1 min−1, respectively, which were 28% and 34% greater than that in the case of P-MIL-101(Cr). Thus, compared with P-MIL-101(Cr), H-MIL-101(Cr) exhibited better removal efficiency and higher levels of activity in the oxidation reactions of indene and 1-dodecene. The unique structure of H-MIL-101(Cr) also contributed to its superior performance in these processes.
New Electrochemical Sensor Based on Hierarchical Carbon Nanofibers with NiCo Nanoparticles and Its Application for Cetirizine Hydrochloride Determination
Authors: Górska Anna , Zambrzycki Marcel , Paczosa-Bator Beata , Piech Robert
Published: May 2022

A new electrochemical sensor based on hierarchical carbon nanofibers with Ni and Co nanoparticles (eCNF/CNT/NiCo-GCE) was developed. The presented sensor may be characterized by high sensitivity, good electrical conductivity, and electrocatalytic properties. Reproducibility of its preparation expressed as %RSD (relative standard deviation) was equal to 9.7% (n = 5). The repeatability of the signal register on eCNF/CNT/NiCo-GCE was equal to 3.4% (n = 9). The developed sensor was applied in the determination of the antihistamine drug—cetirizine hydrochloride (CTZ). Measurement conditions, such as DPV (differential pulse voltammetry) parameters, supporting electrolyte composition and concentration were optimized. CTZ exhibits a linear response in three concentration ranges: 0.05–6 µM (r = 0.988); 7–32 (r = 0.992); and 42–112 (r = 0.999). Based on the calibration performed, the limit of detection (LOD) and limit of quantification (LOQ) were calculated and were equal to 14 nM and 42 nM, respectively. The applicability of the optimized method for the determination of CTZ was proven by analysis of its concentration in real samples, such as pharmaceutical products and body fluids (urine and plasma). The results were satisfactory and the calculated recoveries (97–115%) suggest that the method may be considered accurate. The obtained results proved that the developed sensor and optimized method may be used in routine laboratory practice.
Conductive Layers on a Shrinkable PET Film by Flexographic Printing
Authors: Lepak-Kuc Sandra , Wasilewska Katarzyna , Janczak Daniel , Nowicka Tatiana , Jakubowska Małgorzata
Published: May 2022

In this study, the extremely important and difficult topic of flexographic printing on a heat-shrinkable substrate was taken up. Six commercially available, electrically conductive inks based on silver, copper and graphite nanoparticles were selected and tested upon their applicability for printing on the temperature-sensitive PET material. As a printing substrate, the one-direction heat-shrinkable PET film, with a maximum shrinkage of 78%, was selected. All of the examined inks were subjected to the printing process throughout three different anilox line screens. The tested inks, along with the electric paths printed with them, were subjected to various tests. The main parameters were evaluated, such as printability combined with the rheology tests and ink adhesion to the examined PET substrate together with the electrical conductivity before and after the shrinkage.
Effect of Long-Term Thermal Aging on Microstructure Evolution and Creep Deformation Behavior of a Novel 11Cr-3W-3Co Martensite Ferritic Steel
Authors: Zhao Hongchang , Han Xi , Wang Mingjia , Wang Zixi
Published: May 2022

This paper focused on the microstructure evolution under different thermal aging times at 650 °C and its effect on creep behavior in 11Cr-3W-3Co heat-resistant steel. After short-term thermal aging at 650 °C (>750 h), a Laves phase was found in the regions adjacent to the PAG boundaries, martensitic lath boundaries, and M23C6 carbides, and gradually swallowed adjacent M23C6 carbides with the aging time increased. Higher contents of Si and P are good promoters of the nucleation of the Laves phase during long-term aging. In addition, the coarsening behavior of the Laves phase, M23C6, and MX were investigated. As the aging time increases, the coarsening behavior among precipitated phases in the above-mentioned example exhibits remarkable variability, which is discussed in detail in this paper, and the evolution of the subgrain size was also analyzed in detail. The increasing rate of subgrain size is, in general, consistent with that of the M23C6 carbide size. The evolution of dislocation density in different aging times shows an obvious difference, and the decreasing rate of dislocation density is significantly affected by the precipitated phase after long-term aging time. The creep performance of the material decreases significantly as the aging time increases, which is closely related to the coarsening of the precipitates such as M23C6 carbides and subgrain during long-term aging.
A Finite Element Stress Analysis of a Concical Triangular Connection in Implants: A New Proposal
Authors: Angeles Maslucan Romy , Dominguez John Alexis
Published: May 2022

Conical implant–abutment connections are popular for their stability; however, in other conditions, such as excessive force, implants and abutments can absorb all the stress. Some connections with three points of support can resist more than conical connections. In recent years, different studies has shown that the design of a connection affects its stability. The aim of this study was to analyze and compare the stresses in finite elements (FEs) in a newly proposed conical triangular connection in implants with hexagonal and conical connections. A nonlinear 3D FE parametric model was developed using SOLIDWORKS 2017®. All the connections, i.e., external and internal hexagons, morse taper, conical connection, and the new conical triangular proposal were compared when axial forces of 150, 250, and 350 N were applied to the occlusal. The maximum stress was found in the external hexagon. The maximum stress was concentrated at the level of the neck of the abutment, implant, and bone, except for the morse taper; at the level of the crown and abutment, the lowest stress occurred in the new proposal. Conclusions: The new conical triangular (CT) connection and the conical connection (CC) generate similar stress in the implant, abutment, and crown. However, the CT connection improves the CC by reducing stress at the bone level, adding an advantage to having three retention points.
Shrinkage and Durability of Waste Brick and Recycled Concrete Aggregate Stabilized by Cement and Fly Ash
Authors: Ding Yongfa , Li Hongbo , Zhang Hubiao , Li Sheng , Zhang Xuanshuo , Hua Shudong , Zhao Jing , Tong Yufei
Published: May 2022

To study the shrinkage and freeze-thaw durability of cement-fly-ash-stabilized brick and concrete reclaimed gravel mixture (CFRBCA), recycled gravel was used to replace 100% of the natural gravel in cement-and-fly-ash-stabilized gravel (CFRCA). Five different mixture ratios of recycled brick and recycled concrete were designed. Dry shrinkage tests, temperature shrinkage tests, freeze-thaw cycle tests, ultrasonic tests, and microscopic analyses were then conducted. The test results showed that the water loss rate, dry shrinkage strain, and dry shrinkage coefficient of CFRBCA increased as the age and brick content increased and tended to be stable by approximately the 40th day of age. The reclaimed gravel content had a great influence on the temperature shrinkage of CFRBCA: the temperature shrinkage coefficient first increased and then decreased as the temperature decreased and reached a peak at −10 to 0 °C. The microstructure analysis showed that as the number of freeze-thaw cycles increases, cracks appear and extend in the CFRBCA, hydration products gradually change from dense to loose, and the Ca/Si ratio increases. Through these experiments, the logarithmic relationship model between ultrasonic wave velocity and CFRBCA strength damage, which can better predict the strength loss caused by CFRBCA variation with freeze-thaw cycles, was established. The brick content is the key parameter affecting the durability of the freeze-thawed CFRBCA, and thus the brick slag content should be properly controlled in engineering applications.
Parameter Estimation for Robotic Manipulator Systems
Authors: Zhu Qianfeng , Man Zhihong , Cao Zhenwei , Zheng Jinchuan , Wang Hai
Published: May 2022

In this paper, a novel methodology for estimating the parameters of robotic manipulator systems is proposed. It can be seen that, for the purpose of parameter estimation, the input torque to each joint motor is designed as a linear combination of sinusoids. After the transient responses of joint angles exponentially converge to zero, the steady states of joint angle outputs can be extracted. Since the steady states of joint angles are the equivalent finite Fourier series, the coefficients of the steady state components of joint angles can be further extracted in a fundamental period. With the amazing finding that the steady states contain all dynamic information of manipulator systems, all unknown parameters of the system model can be accurately estimated with the extracted coefficients in finite frequency bands. The simulation results for a two-link manipulator are carried out to illustrate the effectiveness and robustness against measurement noise of the proposed method.
Vision-Aided Brush Alignment Assembly System for Precision Conductive Slip Rings
Authors: Chen Xiaobo , Wang Yukun , Sheng Ying , Yu Chengyi , Yang Xiao , Xi Juntong
Published: May 2022

The alignment precision of manual brush assembly for a precision conductive slip ring is critical to its performance of reliability and service lifetime. Currently, the alignment precision cannot be guaranteed since it largely depends on the operator’s experiences and skill level. In this paper, a machine vision-aided method is proposed to measure the ring groove positions as the brush alignment objective, and track the relative brush position deviation during the manual brush alignment assembly. A vision-aided brush alignment assembly system is also developed to provide quantitative position deviation for the precise alignment of the brush and the ring groove, ensuring higher alignment accuracy and efficiency. The experimental results indicate that, with the developed system, the brush alignment assembly accuracy can be controlled within ±0.02 mm.
Surface Roughness Improvement by Sliding Friction Burnishing of Parts Produced by Selective Laser Melting of Ti6Al4V Titanium Alloy
Authors: Varga Gyula , Dezső Gergely , Szigeti Ferenc
Published: May 2022

Selective laser melting is a frequently used, powder bed fusion additive manufacturing technology for producing metallic parts. However, appropriate surface quality cannot be achieved, so post-processing is often necessary. Subsequent machining of surfaces serves multiple objectives such as improvement of dimensional accuracy, changing surface roughness and modification of the residual stress state for higher surface hardness. Beyond its several advantageous properties, Ti6Al4V material has, as its weaknesses, low tribological behavior and wear resistance. Sliding friction burnishing is a conventional chipless and coolant-free environmentally conscious technology for surface modification that is appropriate for simultaneously decreasing surface roughness and increasing surface hardness. Until now, there has been a research gap regarding the diamond burnishing of selective laser melted Ti6Al4V parts. In this study, we investigated how the surface roughness of selective laser melted parts can be modified via sliding friction burnishing. 2D and 3D characteristics of surface roughness were measured by a chromatic roughness measuring device. Indices of surface roughness improvement were defined and studied as a function of selective laser melting parameters. Optimal manufacturing parameters of laser power—P = 280 W and scanning speed u = 1200 mm/s—for effective surface improvement via burnishing are proposed.
Shifted Brownian Fluctuation Game
Authors: Kim Song-Kyoo (Amang)
Published: May 2022

This article analyzes the behavior of a Brownian fluctuation process under a mixed strategic game setup. A variant of a compound Brownian motion has been newly proposed, which is called the Shifted Brownian Fluctuation Process to predict the turning points of a stochastic process. This compound process evolves until it reaches one step prior to the turning point. The Shifted Brownian Fluctuation Game has been constructed based on this new process to find the optimal moment of actions. Analytically tractable results are obtained by using the fluctuation theory and the mixed strategy game theory. The joint functional of the Shifted Brownian Fluctuation Process is targeted for transformation of the first passage time and its index. These results enable us to predict the moment of a turning point and the moment of actions to obtain the optimal payoffs of a game. This research adapts the theoretical framework to implement an autonomous trader for value assets including stocks and cybercurrencies.