bluuebunny/crossref_metadata_2025 huggingface dataset:
New Therapeutic Targets and Treatment Options for Thrombotic Microangiopathy: Caplacizumab and Ravulizumab
Authors: Chung Clement
Published: July 2020

Objective

To review the efficacy and safety of caplacizumab and ravulizumab for thrombotic microangiopathy.

Data Sources

A literature search from January 2011 to May 2020 was performed using the key terms caplacizumab (or ALX-0681), ravulizumab (or ALXN1210), atypical hemolytic uremic syndrome (aHUS), acquired thrombotic thrombocytopenic purpura (aTTP), and thrombotic microangiopathy.

Study Selection and Data Extraction

Relevant clinical trials and articles in the English language were identified and reviewed.

Data Synthesis

aTTP and aHUS are syndromes of thrombotic microangiopathy manifested by excessive platelet aggregation and endothelial cell destruction, with subsequent thrombocytopenia, hemolysis, and multiorgan failure. Current standard therapy for aTTP is therapeutic plasma exchange (TPE) to remove von Willebrand factor (vWF) multimers and anti-ADAMTS13 autoantibodies. As an adjunctive therapy to TPE, caplacizumab inhibits binding of vWF to platelets and prevents new microthrombi formation. It reduces thromboembolic event rate and days of TPE and delays relapse. Headache and epistaxis were the most common adverse events. aHUS develops because of dysregulation of the alternative complement pathway, followed by constitutive activation of complement components that causes thrombosis and end-organ damage. Short-term initial evaluation with ravulizumab, a long-acting complement inhibitor, demonstrates rapid hematological and renal improvement, with sustained complement inhibition and tolerable adverse effects.

Relevance to Patient Care and Clinical Practice

This review describes the pharmacology, pharmacokinetics, cost consideration, and clinical studies for caplacizumab and ravulizumab for thrombotic microangiopathy. Place of therapy is also discussed.

Conclusion

Targeted therapies with caplacizumab and ravulizumab are expected to reduce the burden of exacerbation, refractory disease, recurrence, and possibly death for thrombotic microangiopathy.
Tibiotalocalcaneal Fusion With Antibiotic Cement-Coated Nails for Refractory Deep Infection After Ankle ORIF
Authors: Herrera-Pérez Mario , Díaz-Fernández Rodrigo , Rendón-Díaz Diego , Boluda-Mengod Juan , Pais-Brito José Luis , Tejero Sergio
Published: July 2020

Background:

Deep infection after open reduction internal fixation (ORIF) of ankle fractures represents a challenge to the orthopaedic surgeon, particularly in patients in whom conventional surgical treatments have failed. The aim of this study was to assess the results of a modified technique of tibiotalocalcaneal fusion using a retrograde locked intramedullary nail covered in cement with antibiotics.

Methods:

Six patients treated using the authors’ technique were analyzed retrospectively. All patients had deep infection after ankle osteosynthesis and several surgical procedures (debridement, external fixation, etc) had failed. Radiographs were analyzed to confirm the healing of the bone. Outcome was measured by maintained construct stability and eradication of infection (no clinical signs of infection and normal values of laboratory markers). The average age of the series was 64.2 (range, 50-75) years, and the average follow-up period was 19.5 (range, 8-41) months.

Results:

Tibiotalocalcaneal stability and eradication of the infection were achieved in all patients, along with the normalization of clinical and radiologic parameters. In the patient who underwent a talectomy, one of the calcaneal locking screws broke, with no clinical repercussions.

Conclusion:

Tibiotalocalcaneal fusion with antibiotic cement-coated retrograde nails was useful in providing clinically acceptable results in the control of chronic infection in complex patients after the failure of previous surgeries.

Level of Evidence:

Level IV, therapeutic study.
The Ups and Downs of BACE1: Walking a Fine Line between Neurocognitive and Other Psychiatric Symptoms of Alzheimer’s Disease
Authors: Ovsepian Saak V. , Horacek Jiri , O’Leary Valerie B. , Hoschl Cyril
Published: July 2020

Although neurocognitive deficit is the best-recognized indicator of Alzheimer’s disease (AD), psychotic and other noncognitive symptoms are the prime cause of institutionalization. BACE1 is the rate-limiting enzyme in the production of Aβ of AD, and one of the promising therapeutic targets in countering cognitive decline and amyloid pathology. Changes in BACE1 activity have also emerged to cause significant noncognitive neuropsychiatric symptoms and impairments of circadian rhythms, as evident from clinical trials and reports in transgenic models. In this study, we consider key characteristics of BACE1 with its contribution to neurocognitive deficit and other psychiatric symptoms of AD. We argue that a growing list of noncognitive mental impairments related to pharmacological modulation of BACE1 might present a major obstacle in clinical translation of emerging therapeutic leads targeting this protease. The adverse effects of BACE1 inhibition on mental health call for a revision of treatment strategies that assume indiscriminate inhibition of this key protease, and stress the need for further mechanistic and translational studies.
Cross-Cultural Adaptation and Psychometric Testing of the Spanish Version of Iceland Health Care Practitioner Illness Beliefs Questionnaire
Authors: Alfaro-Díaz Cristina , Esandi Nuria , Canga-Armayor Navidad , Pardavila-Belio M. Idoia , Pueyo-Garrigues María , Gutiérrez-Alemán Teresa , Canga-Armayor Ana
Published: July 2020

The beliefs of nursing professionals who care for families experiencing illness are fundamental to the quality of the nurse–family relationship and the level of the nurse’s involvement in the therapeutic process of Family Systems Nursing. It is essential to have valid and reliable instruments for assessing nurses’ illness beliefs, especially in the Spanish context where no instruments have been identified to date. The Iceland Health Care Practitioner Illness Beliefs Questionnaire (ICE-HCP-IBQ) is a reliable and valid measure of professionals’ beliefs about their understanding of the meaning of the illness experience of families. The purpose of this study was to adapt and psychometrically test the Spanish version of the ICE-HCP-IBQ ( N = 249 nurses). The exploratory factor analysis showed one-factor solution with good internal consistency (Cronbach’s α = .91) and test–retest reliability ( r = .72, p < .01). This questionnaire is a promising tool for mapping nurse’s illness beliefs and monitoring the effectiveness of family nursing educational interventions in the Spanish context.
Inclusion of Caregivers in Veterans’ Care: A Critical Literature Review
Authors: Boucher Nathan A. , Shepherd-Banigan Megan , McKenna Kevin , Delgado Roxana E. , Peacock Kimberly , Van Houtven Courtney H. , Van Noord Megan , Sperber Nina R.
Published: July 2020

More direct inclusion of informal caregivers (i.e., family, friends) in patients’ care will make care more patient- and family-centered and has the potential to improve overall quality of care for patients. We need to understand what potentially comprises “inclusive care” so that we can define what “inclusive care” is and develop targets for care quality metrics. We conducted a critical literature review to identify key components of “caregiver inclusion.” Focusing on extant literature from 2005 to 2017, 35 papers met inclusion criteria. Directed content analysis with constant comparison was used to identify major themes related to a concept of “inclusive care.” Our analysis indicates that “inclusive care” entails five components: clear definition of caregiver role, system level policies for inclusion, explicit involvement of caregiver, provider assessment of caregiver capability, and mutuality in caregiver–provider communication. We discuss the evidence behind these five components using the Donabedian health care quality conceptual model.
Safety and visual outcomes following Iris-claw phakic intraocular lens bilensectomy
Authors: Vargas Veronica , Marinho Antonio , El Sayyad Fouad , Alio del Barrio Jorge L , Alio Jorge L
Published: July 2020

Purpose:

To evaluate the visual outcomes, safety, efficacy, and causes of bilensectomy for iris fixated phakic intraocular lenses (pIOLs).

Methods:

This was a two center consecutive retrospective study that included 43 eyes that underwent iris-claw pIOL bilensectomy. Patients with follow up less than 4 months were excluded from the study. Uncorrected, corrected visual acuity (UCVA, CDVA), refractive outcomes, efficacy, safety (number of eyes in which the postoperative CDVA was worse than the preoperative CDVA), endothelial cell density (ECD), causes of bilensectomy, the time between the implantation of the pIOL and bilensectomy, intra and postoperative complications were analyzed.

Results:

There was a statistically significant improvement in UCVA and CDVA after surgery ( p = 0.001). The efficacy index was 0.7, four eyes had a postoperative CDVA worse than the preoperative CDVA. The mean spherical equivalent 1 year after bilensectomy was −0.78 ± 1.70 D. There was a statistically significant endothelial cell loss after iris claw lens bilensectomy ( p = .003). Cataract development and endothelial cell loss were the only reasons for bilensectomy. The mean time between pIOL implantation and bilensectomy was 12.2 ± 5.5 years. One patient had corneal edema 8 months after surgery and one had a retinal detachment 11 months after surgery.

Conclusion:

Bilensectomy was successful in improving UCVA and CDVA with an acceptable refractive outcome. Significant loss of ECD was found after surgery. The results recommend a larger ACD as selection criteria when choosing to implant an Artisan lens, and a close postoperative monitoring of the endothelial cell count.
Corneal biomechanical outcome of collagen cross-linking in keratoconic patients evaluated by Corvis ST
Authors: Jabbarvand Mahmoud , Moravvej Zahra , Shahraki Kianoush , Hashemian Hessam , Ghasemi Hamed , Berijani Sahar , Amiri Zahra , Jamali Alireza
Published: July 2020

Purpose:

A 6-month evaluation of the topographic and biomechanical changes induced by corneal collagen cross-linking (CXL) in keratoconic eyes using Pentacam and Corvis ST.

Design:

Longitudinal prospective case series.

Methods:

In this study, 67 eyes of 67 patients with progressive keratoconus (KCN) treated with “Epithelium-off” CXL were evaluated. Patients with stages 1 or 2 of KCN and a corneal thickness of at least 400 μm at the thinnest point were included. Standard ophthalmologic examinations were carried out for all patients. The topographic and biomechanical measurements of the cornea were obtained by Pentacam (Oculus Optikgeräte GmbH, Wetzlar, Germany) and Corvis ST (Oculus Optikgeräte GmbH, Wetzlar, Germany) preoperatively and 6-month postoperatively.

Results:

The mean age of the participants was 21.68 ± 4.23 years. There was significant difference in mean spherical equivalent (SE) before and 6 months after CXL. Uncorrected and best corrected visual acuity improved postoperatively, although not statistically significant. The mean and maximum keratometry showed a significant decrease 6 months after CXL (0.93 ± 0.38 D and 1.43 ± 0.62 D, respectively p < 0.001). Among Corvis ST parameters, first applanation length and velocity (AL1 and AV1) showed statistically significant changes. The radius at highest concavity changed significantly (0.13 ± 0.37 mm mean increase after CXL; p < 0.001). A significant increase was observed in stiffness parameter A1 (SP-A1; p < 0.001) and significant decreases were noted in integrated radius (IR) and deformation amplitude ratio (DAR; p < 0.001).

Conclusion:

Analyzing biomechanical changes after corneal cross-linking can provide basis for efficient KCN treatment. Corvis ST parameters demonstrated changes in corneal biomechanical characteristics indicative of stiffing after CXL.
Feasibility of routine ultrasound-guided percutaneous transluminal angioplasty in the treatment of native arteriovenous fistula dysfunction
Authors: Granata Antonio , Maccarrone Rosario , Di Lullo Luca , Morale Walter , Battaglia Giovanni Giorgio , Di Nicolò Pierpaolo , Bellasi Antonio , Pesce Francesco , Khater Emad , Gesualdo Loreto , Basile Antonio , Gallieni Maurizio
Published: July 2020

Background:

Stenosis is the main cause of arteriovenous fistula failure and is due to neointimal hyperplasia. Percutaneous transluminal angioplasty is the gold standard for patients with vascular access stenosis. The aim of the study was to evaluate the efficacy and safety of ultrasound-guided percutaneous transluminal angioplasty in the treatment of native arteriovenous fistula venous stenosis.

Methods:

The need for intervention was determined by physical examination and duplex ultrasound in 162 patients. All patients with failing or not maturing arteriovenous fistula were treated in the outpatient setting under ultrasound guidance. Procedural success was assessed with repeated post-procedural ultrasound examinations. All procedures were performed under local anesthesia by a single nephrologist and were performed in a single vascular laboratory, while follow-up ultrasound was performed in the dialysis unit of destination.

Results:

Early technical success was obtained in 95.6% of cases (154 of 162). Complications occurred in 22 patients (13.5%) with no major complication requiring surgical or fluoroscopic endovascular intervention. Primary patency at 6 and 12 months was 84% and 69.8%, respectively. Risk factors for arteriovenous fistula failure/secondary percutaneous transluminal angioplasty were vascular access low blood flow rate and vintage, as well as the need for thrombolysis during the first percutaneous transluminal angioplasty.

Conclusion:

Ultrasound-guided percutaneous transluminal angioplasty is a valuable tool to treat vascular access stenosis.
A bedside rescue method for retrieving retained guidewires: The ‘Suck Out’ technique
Authors: Mariyaselvam Maryanne Z A , Patel Vikesh , Sawyer Adam , Richardson James A , Dean Jonathan , Blunt Mark C , Young Peter J
Published: July 2020

Background:

Central venous catheter guidewire retention is classed as a ‘never event’ in the United Kingdom, with the potential for significant patient harm. If the retained guidewire remains within the central venous catheter lumen, bedside techniques may facilitate guidewire retrieval. However, these techniques may be ineffective if the guidewire has already passed below skin level. We investigated a novel ‘suck out’ technique for bedside guidewire retrieval and compared this against traditional retrieval methods.

Methods:

Simulation 1: in a benchtop model, seven different central venous catheters had their corresponding guidewire placed in the last 2 cm of the catheter tip which was immersed horizontally in fluid. A 50-mL syringe was attached to the distal lumen central venous catheter hub and suction applied for 5 s, and the distance of guidewire retraction was recorded. Simulation 2: a central venous catheter guidewire was intentionally retained within the catheter at either 5 cm above or below skin level in a pigskin model. Simple catheter withdrawal, catheter clamping withdrawal and the ‘suck out’ method were compared for efficacy using Fisher’s exact test.

Results:

Simulation 1: retained guidewires were retracted by 13 cm on average. Simulation 2: when guidewires were retained 5 cm above skin level, all retrieval methods were 100% effective; however, when retained 5 cm below skin level, simple catheter withdrawal was ineffective, clamping and withdrawal was only 10% effective and the ‘suck out’ technique was 90% effective (p < 0.001).

Conclusion:

The ‘suck out’ technique can effectively retract guidewires retained within central venous catheter lumens and demonstrates superiority over traditional methods of retained guidewire extraction in simulated models.
Avoiding hemodialysis access–induced distal ischemia
Authors: Horst Vernon D , Nelson Peter R , Mallios Alexandros , Kempe Kelly , Pandit Viraj , Kim Hyein , Jennings William C
Published: July 2020

Timely creation and maintenance of a safe and reliable vascular access is essential for hemodialysis patients with end-stage renal disease. Hemodialysis access–induced distal ischemia (HAIDI) is a recognized complication of arteriovenous fistulas and grafts that may result in serious or even devastating consequences. Avoiding such complications is clearly preferred over treatment of HAIDI once established. Proper recognition of patients at increased risk of HAIDI includes careful pre-operative evaluation of the patient’s medical and surgical history along with physical examination and imaging to determine a plan for creating a functional permanent access while minimizing the risk of distal ischemia. Our aim is to review identifying characteristics of individuals at risk of HAIDI and provide recommendations regarding pre-operative assessment. Vascular access options and techniques are suggested for establishing a functional vascular access without distal ischemia for such patients.
Parents’ perceptions and concerns about physical activity participation among adolescents with autism spectrum disorder
Authors: Arnell Susann , Jerlinder Kajsa , Lundqvist Lars-Olov
Published: July 2020

The parents of adolescents with autism spectrum disorder have a vital and proactive role in encouraging healthy physical activity habits, and they possess important knowledge about the adolescents’ needs when it comes to enhancing participation in physical activity. But promoting healthy physical activity habits in adolescents can be difficult. The purpose of this study was thus to describe parents’ perceptions of their adolescent child’s participation in physical activity and to describe the parental role in promoting such participation. Twenty-eight parents of adolescents aged 12–16 years with autism spectrum disorder were interviewed. The interviews were analyzed using an inductive content analysis approach. The parents described how challenging participation in physical activities could be for their adolescents. Despite this, they wanted to see their children participate more in physical activity but found the promotion of physical activity to be an overwhelming task that was difficult to cope with on their own. The results reveal a need for support and collaborative efforts among different actors to give these issues increased priority in order to promote the adolescents’ physical activity participation.

Lay abstract

What is already known about the topic? The parents of adolescents with autism spectrum disorder have a vital and proactive role in encouraging healthy physical activity habits. But promoting healthy physical activity habits in adolescents can be difficult. The purpose of this study was thus to describe the parental perceptions of their adolescent child’s participation in physical activity and to describe the parental role in promoting such participation. Twenty-eight parents of adolescents aged 12–16 years with autism spectrum disorder were interviewed. What this paper adds? The parents described how challenging participation in physical activities could be for their adolescents. Despite this, they wanted to see their children participate more in physical activity but found the promotion of physical activity to be an overwhelming task that was difficult to cope with on their own. Implications for practice, research, or policy. The results reveal a need for support and collaborative efforts among different actors to give these issues increased priority in order to promote the adolescents’ physical activity participation.
Originality versus proximity: An explorative study of audience reactions to monolingual film remakes
Authors: Cuelenaere Eduard
Published: July 2020

Striving for a more holistic approach of the field that studies film remakes, this article analyses the reception context of the monolingual film remake practice in the Low Countries. Instead of assuming how audiences define, perceive, experience, and evaluate Dutch-Flemish film remakes, this article explores these aspects empirically by analysing data from four focus groups. Given the cultural proximity between the Netherlands and Flanders, the study equally inquires if (and why) Dutch and Flemish audiences prefer their local film versions. It is found that participants do not share a common definition of the artefact and understand the label differently from scholars or people working in the industry. Participants were highly critical of film remakes and generally preferred the local version. However, it appeared that the latter depended on whether they knew which version was the ‘original’.
Globalising the local in children’s television for the post-network era: How Disney+ and BBC Studios helped Bluey the Australian cattle dog jump the national fence
Authors: Potter Anna
Published: July 2020

Online television services are changing the way television is funded, produced and experienced by its national and global audiences, with profound cultural implications. The case study presented here of Australian production company Ludo Studio and the animated series Bluey provides valuable insights into the increasingly complex interactions between the local and the global in television production, and the cultural counterflows they can engender. Television made for online distribution can encompass national policy settlements, funding bodies and public service media as well as globally oriented distribution arrangements on SVODs such as Disney +, Netflix and Amazon Prime Video. Drawing on interview material with Bluey’s key creatives, this research identifies how globalising influences are both shaping and amplifying the local in children’s television production cultures.
Interest matters: The effects of constructive news reporting on Millennials’ emotions and engagement
Authors: Hermans Liesbeth , Prins Tineke
Published: July 2020

News media seem to have insufficient knowledge on how to reach Millennials. An important question is how professional journalism can prevent the disillusion that a growing number of Millennials experience when consuming news. Constructive journalism is often propagated as a way to improve the well-being and engagement of readers. To test this, the current study investigated the effects of constructive news on emotions and online engagement. In an experiment, 20–40-year-old participants ( n = 341) read a story containing constructive elements or not. Findings showed constructive news elicited lower levels of negative and higher levels of positive and inspirational emotional responses. Furthermore, reading constructive news partly affected readers’ actual online behaviour: Millennials who read the constructive news ‘liked’ this news more often. These results suggest that constructive journalism might be a viable strategy to attract younger news users, especially when the news topic is relevant to their lives.
Using genetic algorithm to automate the generation of an open-plan office layout
Authors: Chen Chen , Chacón Vega Ricardo Jose , Kong Tiong Lee
Published: July 2020

Today, the concept of open plan is more and more widely accepted that many companies have switched to open-plan offices. Their design is an issue in the scope of space layout planning. Although there are many professional architectural layout design software in the market, in the real life, office designers seldom use these tools because their license fees are usually expensive and using them to solve an open-plan office design is like using an overly powerful and expensive tool to fix a minor problem. Therefore, manual drafting through a trial and error process is most often used. This article attempts to propose a lightweight tool to automate open-plan office layout generation using a nested genetic algorithm optimization with two layers, where the inner layer algorithm is embedded in the outer one. The result is enhanced by a local search. The main objective is to maximize space utilization by maximizing the size of the open workspace. This approach is different from its precedents, in that the location search is conducted on a grid map rather than several pre-selected candidate locations. Consequently, the generated layout design presents a less rigid workstation arrangement, inviting a casual and unrestrictive work environment. The real potential of the approach is reflected in the productivity of test fits. Automating and simplifying the generation of layouts for test fits can tremendously decrease the amount of time and resources required to generate them. The experimental case study shows that the developed approach is powerful and effective, making it a totally automated process.
Accuracy of Online Pictorial Resources for Common Hand Surgery Procedures
Authors: Park Kitae E. , Allam Omar , Kim Samuel , Prsic Adnan
Published: July 2020

Background: Online medical platforms can provide patients with easily accessible information and greater opportunities to self-advocate. However, the lack of quality control and presence of inaccurate information can lead to miscommunications between the physician and the patient. The objective of this study was to examine the quality and accuracy of online pictorial information regarding common hand conditions. Methods: Medical image information was searched on the search engine Google ( http://www.google.com ) using the terms “de Quervain’s tenosynovitis,” “carpal tunnel syndrome,” and “trigger finger.” The first 20 illustrations to appear on the search were recorded along with the type of source. The images were then examined for veracity of information conveyed. Results: Sixty images were collected from 48 different Web sources. Nonacademic/private medical institutions were the most common image source (35%). The rate of erroneous images was 40%. Web sites of academic hospitals were most frequently the source of incorrect images. Of the conditions, trigger finger had the highest rate of errors (55%), most commonly occurring in positioning of the annular pulleys. The search results did not contain any Web sites from professional hand societies. Conclusion: The quality of online medical information is a significant but often overlooked aspect of health professional and patient education. This study demonstrates the prevalence of incorrect information online and the misunderstandings that patients can have about common conditions. Higher quality online resources are needed to improve patient education and patient-physician interactions. Avenues for improvement are to provide greater accessibility of educational resources offered by professional hand surgery organizations.
Migrant modernists: The making of national architects in Lithuania in the 1930s and their survival strategies in the 1950s
Authors: Drėmaitė Marija
Published: July 2020

The new national states that emerged on the European map in 1918 following the collapse of the great European empires in the wake of World War I became enthusiastic participants in the race to modernise, hoping to keep pace with global trends and become more European in the process. Renewal was the central goal of many European cities. This was particularly so in the newly restored cities or those newly designated as administrative capitals such as Kaunas, which became the provisional capital of the Republic of Lithuania from 1919 to 1939. These cities faced similar challenges: ridding themselves of imperial pasts, architectural legacies and symbols, changing their urban environment, creating new political centres and constructing new government facilities. The question of national architects became similarly important. Through the lives of the modern architects who were compelled to change their citizenship or suffered exile, forced migration or genocide, we can study the effect of social and political change, and in particular political ruptures. This paper follows how architects, collectively as well as individually, developed as a modern group of socially engaged intellectuals in 1930s. It then considers how they reacted to political changes, such as the first communist occupation of Lithuania in 1940, the subsequent Nazi occupation in 1941, and the return of the Red Army in 1944, when most of the architects in private practice emigrated to the West. Finally, it illustrates how those architects who remained adapted to the new political rule in Soviet Lithuania in the 1950s.
Tribological capabilities of chiral nematic liquid crystal additives in mineral motor oil
Authors: Mokshin Vadim
Published: July 2020

This article presents the results of experimental investigation of tribological properties of commercial mineral motor oil with chiral nematic (also known as twisted nematic or cholesteric) liquid crystal additives. Cholesteryl stearate and valerate (fatty acid cholesterol esters) liquid crystals were used as oil additives in investigation. Tribological experiments were performed using a block-on-disc-type tribo-test machine at constant experiment time and sliding velocity conditions. The load (contact pressure), concentration of liquid crystalline additive in oil and Rz roughness of steel–steel friction surfaces were taken as variable parameters. The mean coefficient of friction of steel–steel friction pair lubricated by oil with and without liquid crystal additives and near-contact temperature were taken as dependent variables. Regression equations were then derived for each lubricant and tribological efficiency of liquid crystals as oil additives was evaluated. It was established that coefficient of friction of steel–steel friction pair and near-contact temperature are reduced to about a half in the presence of liquid crystal additives. Results of tribological experiments show that tribological efficiency of liquid crystals as oil additives increases with increase in their molecular mass.
Seismic risk analysis of concrete moment-resisting frames against near-fault earthquakes
Authors: Khojastehfar Ehsan , Mirzaei Aminian Farzad , Ghanbari Hamid
Published: July 2020

Characteristics of earthquake strong ground motions play an important role in the calculation of seismic-induced risk imposed on the structures. Distinguished features exist in movements recorded near seismic sources, as a result of a substantial amount of energy in a short period of record arrival time. In this article, seismic risk analysis of concrete moment-resisting frames due to near-fault strong ground motion is calculated and compared with that of caused by far-field strong ground motions. To achieve this goal, three moment-resisting frames with 4, 6, and 10 stories were designed based on international seismic design code. These frames are modeled applying modified Ibarra–Krawinkler moment–rotation nonlinear model in which strength and stiffness deterioration are involved. Seismic risk analysis of the frames is implemented using the Pacific Earthquake Engineering Research Center approach. Through this approach, probabilistic seismic hazard, probabilistic structural demand, probabilistic structural damage, and probabilistic loss curves are combined. Mean annual frequency of exceedance of seismic-induced losses presents probabilistic seismic risk of the sampled frames. According to the achieved results, the four-story frame (representative of low-rise frames) is more prone to be affected by near-fault strong ground motions in view of calculated seismic-induced risks.
Synovial Fluid Biomarkers in Knee Osteoarthritis: A Systematic Review and Quantitative Evaluation Using BIPEDs Criteria
Authors: Boffa Angelo , Merli Giulia , Andriolo Luca , Lattermann Christian , Salzmann Gian M. , Filardo Giuseppe
Published: July 2020

Objective

The aim of this systematic review was to analyze the evidence about the efficacy of the several synovial fluid (SF) biomarkers proposed for knee osteoarthritis (OA), categorizing them by both molecular characteristics and clinical use according to the BIPEDs criteria, to provide a comprehensive and structured overview of the current literature.

Design

A systematic review was performed in May 2020 on PubMed, Cochrane Library, and Embase databases about SF biomarkers in patients with knee OA. The search was limited to articles in the last 20 years on human studies, involving patients with knee OA, reporting SF biomarkers. The evidence for each selected SF biomarker was quantified according to the 6 categories of BIPEDs classification.

Results

A total of 159 articles were included in the qualitative data synthesis and 201 different SF biomarkers were identified. Among these, several were investigated multiple times in different articles, for a total of 373 analyses. The studies included 13,557 patients with knee OA. The most promising SF biomarkers were C4S, IL-6, IL-8, Leptin, MMP-1/3, TIMP-1, TNF-α, and VEGF. The “burden of disease” and “diagnostic” categories were the most represented with 132 and 106 different biomarkers, respectively.

Conclusions

The systematic review identified numerous SF biomarkers. However, despite the high number of studies on the plethora of identified molecules, the evidence about the efficacy of each biomarker is supported by limited and often conflicting findings. Further research efforts are needed to improve the understanding of SF biomarkers for a better management of patients with knee OA.
Autologous Minced Cartilage Implantation for Treatment of Chondral and Osteochondral Lesions in the Knee Joint: An Overview
Authors: Salzmann Gian M. , Ossendorff Robert , Gilat Ron , Cole Brian J.
Published: July 2020

Cartilage defects in the knee are being diagnosed with increased frequency and are treated with a variety of techniques. The aim of any cartilage repair procedure is to generate the highest tissue quality, which might correlate with improved clinical outcomes, return-to-sport, and long-term durability. Minced cartilage implantation (MCI) is a relatively simple and cost-effective technique to transplant autologous cartilage fragments in a single-step procedure. Minced cartilage has a strong biologic potential since autologous, activated non-dedifferentiated chondrocytes are utilized. It can be used both for small and large cartilage lesions, as well as for osteochondral lesions. As it is purely an autologous and homologous approach, it lacks a significant regulatory oversight process and can be clinically adopted without such limitations. The aim of this narrative review is to provide an overview of the current evidence supporting autologous minced cartilage implantation.
Cytomorphological patterns of palpable breast lesions diagnosed on fine needle aspiration cytology in females
Authors: Sharif Aamir , Tabassum Tahira , Riaz Muhammad , Akram Muhammad , Munir Naveed
Published: July 2020

Breast cancer is the most common malignant tumor and is a leading cause of death worldwide. This study was planned to find out the frequencies of various types of lesions from palpable breasts of female patients through fine needle aspiration cytology. This retrospective study was carried out during December 2017 to May 2018 on 100 female patients presenting with palpable breast mass at University Medical Complex & Research Center, Sargodha, Pakistan. Following written informed consent from patients, a detailed history, patient age, and clinical examination were recorded. The fine needle aspiration cytology was performed and aspirates were processed following the standard methods for cytopathological examination. The cases were grouped according to the five tier reporting format for breast lesion (C1–C5) laid down by the International Academy of Cytologists (IAC) in 2016. The spectrum of breast lesions on cytomorphological interpretation was 54% benign (C2), 2% atypia/suspicious probably benign (C3), 3% suspicious probably malignant (C4) and 41% malignant (C5). Inadequate/insufficient material (C1) was not included in the study. In this study, the specificity, sensitivity, negative and positive predictive value, and diagnostic accuracy of fine needle aspiration cytology were 100%, 91.11%, 98.18%, 100%, and 98.96%, respectively. In benign lesions, maximum cases were of fibroadenoma (24%) followed by fibrocystic disease (4%), lipoma (3%) while benign phyllodes tumor and galactocele were only 1% each. Breast mass was the chief presenting complaint. Breast cancer was commonest among all the morphological patterns of breast lesions followed by fibroadenoma. This study supports that cytological examination using fine needle aspiration cytology is an economical, rapid, easy and valuable diagnostic tool.
Financial Literacy and Financial Risk Tolerance of Individual Investors: Multinomial Logistic Regression Approach
Authors: Bayar Yılmaz , Sezgin H. Funda , Öztürk Ömer Faruk , Şaşmaz Mahmut Ünsal
Published: July 2020

Financial risk tolerance is one of the important factors affecting the financial investment decisions of individuals and institutional investors and a crucial factor of financial planning and financial counseling. It is therefore necessary to determine the major determinants of risk tolerance. In this article, we researched the impact of financial literacy level and demographic characteristics on the financial risk tolerance of the individuals in the sample of Usak University staff, using a multinomial logistic regression analysis and retrieving data through the questionnaire method. Multinomial logistic regression is an extension of binary logistic regression, allowing for three or more categories of the dependent variable. The findings of the empirical analysis reveal that financial literacy and demographic characteristics of age, gender, education, and income levels are significant determinants of financial risk tolerance. In this regard, the improvements in the financial literacy of the individuals through various education programs will probably raise the demand of financial products with different risk characteristics and in turn contribute to the development of financial sector.
The Effect of Social Capital on Tacit Knowledge-Sharing Intention: The Mediating Role of Employee Vigor
Authors: Cai Yang , Song Youming , Xiao Xia , Shi Wendian
Published: July 2020

The role of social capital in predicting knowledge sharing has received considerable attention in research. However, very limited research has investigated the mechanisms mediating this relationship. To address this important gap in knowledge, the purpose of this study was to examine employee vigor as a psychological mechanism mediating the effect of social capital on tacit knowledge-sharing intention. Data collected from 209 employees in multiple industries in China were empirically tested by using structural equation modeling analysis. The results show that social capital positively affects emotional energy, which then positively influences the intention to share tacit knowledge. However, neither physical strength nor cognitive liveliness mediates the path through which social capital impacts tacit knowledge-sharing intention. Our research findings unpack the impact mechanism of social capital on tacit knowledge-sharing intention, and provide practical insights into how to use social capital to facilitate employees to share tacit knowledge in organizations.
Book review: Monika Halan, Let’s Talk Money: You’ve Worked Hard for It, Now Make It Work for You
Authors: Hiremath Gourishankar S.
Published: July 2020

Monika Halan, Let’s Talk Money: You’ve Worked Hard for It, Now Make It Work for You. New Delhi: Harper Business, 2018. 204 pp., ₹399.00 (Paperback). ISBN-10: 9789352779390; ISBN-13: 978-9352779390.
Reduced Energy Barrier for Li+ Transport Across Grain Boundaries with Amorphous Domains in LLZO Thin Films
Authors: Zhu Yanlin , Wu Shuai , Pan Yilan , Zhang Xiaokun , Yan Zongkai , Xiang Yong
Published: July 2020

The high-resistive grain boundaries are the bottleneck for Li+ transport in Li7La3Zr2O12 (LLZO) solid electrolytes. Herein, high-conductive LLZO thin films with cubic phase and amorphous domains between crystalline grains are prepared, via annealing the repetitive LLZO/Li2CO3/Ga2O3 multi-nanolayers at 600 °C for 2 h. The amorphous domains may provide additional vacant sites for Li+, and thus relax the accumulation of Li+ at grain boundaries. The significantly improved ionic conductivity across grain boundaries demonstrates that the high energy barrier for Li+ migration caused by space charge layer is effectively reduced. Benefiting from the Li+ transport paths with low energy barriers, the presented LLZO thin film exhibits a cutting-edge value of ionic conductivity as high as 6.36 × 10−4 S/cm, which is promising for applications in thin film lithium batteries.
Disruption of a Rice Chloroplast-Targeted Gene OsHMBPP Causes a Seedling-Lethal Albino Phenotype
Authors: Liu X. , Cao P. H. , Huang Q. Q. , Yang Y. R. , Tao D. D.
Published: July 2020

Background

Chloroplast development is coordinately regulated by plastid- and nuclear-encoding genes. Although many regulators have been reported to be involved in chloroplast development, new factors remain to be identified, given the complexity of this process.

Results

In this study, we characterized a rice mutant lethal albinic seedling 1(las1)form of a 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (OsHMBPP) that was targeted to the chloroplasts. The LAS1 mutation caused the albino lethal phenotype in seedlings. Transmission electron microscopy indicated that las1 were defective in early chloroplast development. LAS1 is preferentially expressed in leaves, implying its role in controlling chloroplast development. The expression levels of many chloroplast-encoded genes were altered significantly in las1. The expression levels of nuclear-encoded gene involved in Chl biosynthesis were also decreased in las1. We further investigated plastidic RNA editing in las1 and found that the edit efficiency of four chloroplast genes were markly altered. Compared with WT, las1 exhibited defective in biogenesis of chloroplast ribosomes.

Conclusions

Our results show that LAS1/OsHMBPP plays an essential role in the early chloroplast development in rice.
The wheat pathogen Zymoseptoria tritici senses and responds to different wavelengths of light
Authors: McCorison Cassandra B. , Goodwin Stephen B.
Published: July 2020

Background

The ascomycete fungus Zymoseptoria tritici (synonyms: Mycosphaerella graminicola, Septoria tritici) is a major pathogen of wheat that causes the economically important foliar disease Septoria tritici blotch. Despite its importance as a pathogen, little is known about the reaction of this fungus to light. To test for light responses, cultures of Z. tritici were grown in vitro for 16-h days under white, blue or red light, and their transcriptomes were compared with each other and to those obtained from control cultures grown in darkness.

Results

There were major differences in gene expression with over 3400 genes upregulated in one or more of the light conditions compared to dark, and from 1909 to 2573 genes specifically upregulated in the dark compared to the individual light treatments. Differences between light treatments were lower, ranging from only 79 differentially expressed genes in the red versus blue comparison to 585 between white light and red. Many of the differentially expressed genes had no functional annotations. For those that did, analysis of the Gene Ontology (GO) terms showed that those related to metabolism were enriched in all three light treatments, while those related to growth and communication were more prevalent in the dark. Interestingly, genes for effectors that have been shown previously to be involved in pathogenicity also were upregulated in one or more of the light treatments, suggesting a possible role of light for infection.

Conclusions

This analysis shows that Z. tritici can sense and respond to light with a huge effect on transcript abundance. High proportions of differentially expressed genes with no functional annotations illuminates the huge gap in our understanding of light responses in this fungus. Differential expression of genes for effectors indicates that light could be important for pathogenicity; unknown effectors may show a similar pattern of transcription. A better understanding of the effects of light on pathogenicity and other biological processes of Z. tritici could help to manage Septoria tritici blotch in the future.
Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416
Authors: Spanelova Petra , Jakubu Vladislav , Malisova Lucia , Musilek Martin , Kozakova Jana , Papagiannitsis Costas C. , Bitar Ibrahim , Hrabak Jaroslav , Pantosti Annalisa , del Grosso Maria , Zemlickova Helena
Published: July 2020

Background

The resistance of Streptococcus pneumoniae to macrolides is becoming an increasingly important issue and thus it is important to understand the genetics related to adaptation of this species to the widespread use of antibiotics in Europe. The 58 isolates of S. pneumoniae belonging to sequence type (ST) 416 and serotype 19A and to several different phenotypes originated from Italy, Portugal and Czech Republic were thus sequenced on Illumina MiSeq. The aim of the study was to describe genetical origine of isolates, investigate their macrolide resistance and suggest reasons for spread of ST416 in the Czech Republic.

Results

Investigation of genes associated with serotype determined serotype switch between 15B and 19A serotypes and core genome multilocus sequence typing (cgMLST) confirmed the origine of concerned isolates in Netherlands15B-37 clone. Inspected genomes proved variability of genes associated with the macrolide resistance even within closely genetically relative isolates.

Conclusions

Participation of 19A/ST416 on the spread of Netherlands15B-37 is accompanied by serotype switch between 19A and 15B serotypes and with acquisition of genes involved in macrolide resistance to the clone that was originally macrolide susceptible. There is evident tendency to interchanging and modifications of these and surrounding genes, that could lead to accelerate spreading of this sequence type in regions with high macrolide consumption.
Correlation of drug resistance with single nucleotide variations through genome analysis and experimental validation in a multi-drug resistant clinical isolate of M. tuberculosis
Authors: Bhattacharyya Kausik , Nemaysh Vishal , Joon Monika , Pratap Ramendra , Varma-Basil Mandira , Bose Mridula , Brahmachari Vani
Published: July 2020

Background

Genome sequencing and genetic polymorphism analysis of clinical isolates ofM. tuberculosisis carried out to gain further insight into molecular pathogenesis and host-pathogen interaction. Therefore the functional evaluation of the effect of single nucleotide variation (SNV) is essential. At the same time, the identification of invariant sequences unique toM. tuberculosiscontributes to infection detection by sensitive methods. In the present study, genome analysis is accompanied by evaluation of the functional implication of the SNVs in a MDR clinical isolate VPCI591.

Result

By sequencing and comparative analysis of VPCI591 genome with 1553 global clinical isolates ofM. tuberculosis(GMTV and tbVar databases), we identified 141 unique strain specific SNVs. A novel intergenic variation in VPCI591 in the putative promoter/regulatory region mapping betweenembC(Rv3793) andembA(Rv3794) genes was found to enhance the expression ofembAB, which correlates with the high resistance of the VPCI591 to ethambutol. Similarly, the unique combination of three genic SNVs in RNA polymerase β gene (rpoB) in VPCI591 was evaluated for its effect on rifampicin resistance through molecular docking analysis.

The comparative genomics also showed that along with variations, there are genes that remain invariant. 173 such genes were identified in our analysis.

Conclusion

The genetic variation inM. tuberculosisclinical isolate VPCI591 is found in almost all functional classes of genes. We have shown that SNV inrpoBgene mapping outside the drug binding site along with two SNVs in the binding site can contribute to quantitative change in MIC for rifampicin. Our results show the collective effect of SNVs on the structure of the protein, impacting the interaction between the target protein and the drug molecule inrpoBas an example. The study shows that intergenic variations bring about quantitative changes in transcription inembABand in turn can lead to drug resistance.