bluuebunny/crossref_metadata_2025 huggingface dataset:
Influences of deposition conditions on atomic layer deposition films for enhanced performance in perovskite solar cells
Authors: Yu Bohao , Zhang Jiawen , Yang Yuzhao , Yu Dingshan , Mai Yaohua , Chen Xudong
Published: May 2024

Atomic layer deposition (ALD) is a key technology for fabricating functional layers in perovskite solar cells, as it can deposit pinhole-free films with atomic-level thickness and tunable composition on high-aspect-ratio surfaces. Various deposition conditions have significant effects on the growth, physical, and chemical properties of ALD films, which, in turn, critically influences the performance of associated devices. Here, we review the reaction mechanisms underlying ALD and summarize how variables, such as precursors, deposition temperatures, and substrates, impinge upon the quality of ALD films and the related devices. We emphasize the role of substrate in determining the nucleation and growth behavior of ALD films, which has been overlooked in previous reviews. Finally, we highlight the potential application of ALD in efficient perovskite solar cells in terms of carrier transport, encapsulated, and buffer layers, especially for tandem cells.
WEBSITE DESIGN INFORMATION SYSTEM OF RECORDING OFFENS AND PUNISHMENT STUDENTS SMK MUHAMMADIYAH 8 SILIRAGUNG USE WATERFALL DEVELOPMENT METHOD
Authors: Martadina Putri Julisa , Aslamiyah Sulaibatul
Published: May 2024

Educational institutions as learning centers are prepared so they keep up through developments, especially regarding the use of information systems which are expected to facilitate the completion of work effectively and in an efficient time so institutions can improve their quality achievements. In this case, SMK Muhammadiyah 8 Siliragung experienced issues in the counselling division regarding recording violations and sanctions which required plenty of time to process and search for the required documents manually. To overcome issues, an information system for recording, as well as storing the history of violations carried out in a computerized manner efficiently to search for history data. This system development uses waterfall development methodology with a MySQL storage database and WEB-based PHP programming language. This research succeeded in identifying the needs through data collection methods, analysis of needs planning and observation meanwhile the system is equipped with a login and logout, a student data, a record, an administration letter, as well as a problematic student data report feature with a violation filter. This information system have been to overcome the issues.
Microbiological quality of dehydrated aromatic condiments and spices used in Food: the danger of contamination and level of toxigenic agents
Authors: Garcia Nascimento Amanda Alfaro , Oliveira Lopez Beatriz de , da Silva Bianka Moreira , Munaretto Mariana Leticia , de Souza Roseli , Oliveira Nazário Cláudia Pedroso de , Vivi Oliveira Viviane Karolina , de Paula Cristiane Coimbra , da Silva Maia Margareth Léa , Leite Júnior Diniz Pereira
Published: May 2024

Thirteen types of condiments and aromatic spices (cloves, cinnamon sticks, star anise, fennel, calabrian pepper, ginger, garlic, cumin, black pepper, turmeric, bay leaves, oregano and nutmeg) from street markets and of supermarkets in the city of Cuiabá – MT, Brazil, were evaluated for contamination by fungi and bacteria. Of the 78 plates containing microbiological samples, 68 (87.2%) presented a positive result, isolating 58 species, 43 (74.1%) of filamentous fungal species, 10 (17.2%) of yeast-like fungal species, with two groups identified only at the genus level and 5 (8.6%) bacterial species identified. The values of colony-forming units per gram of product (CFU/g) detected in samples of condiments sold in free fairs and industrialized markets were higher than those of the federal reference standard, varying up to 8.2×104 CFU/g. There was no significant difference between these values. Aspergillus spp. was the most isolated genus, with the species Aspergillus niger predominating, followed by species from the genus Penicillium, Cladosporium and Fusarium. Among the yeasts found, the most prevalent genera were Candida spp., Saccharomyces spp. and Rodothorula spp. with the highest amount of CFU/g isolated in cinnamon bark samples. The bacteria were the least isolated, with the highest CFU/g value coming from the cumin Streptococcus gengivalis (3.2x103). The condiments analyzed in this study showed contamination by potentially pathogenic fungi and mycotoxin producers, the isolated bacteria may pose a risk to the health of consumers.
Effect of Plant Growth Regulators on Dendrobium orchids cv. Sonia 17 for Vegetative Growth Under Shade Net Conditions in High Altitude Tribal Zone of Andhra Pradesh, India
Authors: Sanghamitra M. , Babu J. Dilip , Bhagavan B. V. K. , Suneetha D. R. Salomi
Published: May 2024

The present investigation entitled “Effect of plant growth regulators on Dendrobium orchid cv. Sonia 17 for vegetative growth and flower yield under shade net conditions in high altitude tribal zone of Andhra Pradesh” was carried out at Horticulture Research station, Chintapalli, Visakhapatnam district of Andhra Pradesh during two consecutive years of 2016-17 and 2017-18. The experiment was laid out in a completely randomized design with 13 treatments and each treatment replicated thrice. The data recorded on various vegetative parameters viz., Plant height, Number of leaves per plant, Number of shoots per plant, Shoot girth, Leaf length, Leaf width and were statistically analyzed. Significant differences were observed among different plant growth regulators on various vegetative parameters in Dendrobium orchids cv. Sonia 17. Among all the treatments GA3 @ 50 ppm showed significantly best results with respect to Plant height, Number of leaves per plant, Number of shoots per plant, Leaf length and Leaf breadth and Shoot girth was recorded more with application of Benzyl adenine 200 ppm.
Management of Root-Knot Nematodes in Peas Using Bacterial and Fungal Bio-Agents: A Review
Authors: Chanu Lankubee Moirangthem , Sumita Kshetrimayum , Arun Singh Yengkhom , Atul Patil Abhiraj
Published: May 2024

Pea, Pisum sativum L. is one of the most important vegetable crops grown in rabi season throughout the world. It is cultivated worldwide over 5.9 million hectares with a production of about 11.7 million tons. In India, it is grown over 0.7 million hectares yielding about 0.6 million tons. Among various obstacles (biotic stress) in cultivating this crop, root-knot nematode (RKN) which is one of the most economically important plant-parasitic nematodes (PPNs) has been reported to cause severe yield losses of up to 20%-56%. RKNs are polyphagous, sedentary endoparasites which is estimated to cause an annual loss of $ 78 billion in agricultural production around the world. In order to control the infection level, frequent and excessive application of chemical nematicides have caused high toxicity level to the soil ecosystems as well as to the environment. An alternative approach of application i.e., biological control agents is an environmentally safe and effective method for sustainable management of RKNs. Among various bioagents, fungal and bacterial agents were reported to reduce RKN density by inhibiting egg hatching, repelling, immobilizing and killing J2s. Nematophagous fungi are capable of capturing, killing, and digesting nematodes. As a group of important natural enemies of nematode pests, nematophagous bacteria also exhibit diverse modes of action including parasitizing, producing toxins, antibiotics, enzymes, competing for nutrients, inducing systemic resistance of plants and promoting plant health.
Effect of Concentrate Feeding Frequency on Hematological Parameters of female Sirohi Goat kids
Authors: Choudhary Karishma , Palsaniya Vinod Kumar , Sharma M. C. , Saini B. S.
Published: May 2024

Goat farming is a significant contributor to India's livestock sector, with Rajasthan being a prominent region in terms of goat population. Nutrition and feeding practices are vital aspects of goat husbandry, influencing growth, health, and productivity. This research explores the effects of feeding frequency of concentrate on hematological parameters in growing female Sirohi goat kids. The experiment was conducted over 90 days, and examined three feeding groups viz., T1: feeding once a day, T2: feeding twice a day, and T3: feeding thrice a day. Hemoglobin concentration, red blood cell count, white blood cell count, packed cell volume, and erythrocyte sedimentation rate were measured at fifteen-day intervals. Three times a day feeding (T3) significantly (P<0.05) increased Hb (gm/dl) due to haemodilution by increasing water intake with feeding frequencies of concentrate compared with other groups. The PCV (%) and RBC count were significantly (P<0.01) higher T3 group but WBC count and ESR values were non-significantly affected by the feeding frequency of the concentrate. Results demonstrate significant variations in hematological profiles among the feeding frequency groups, highlighting the importance of tailored feeding strategies in goat farming.
Performance of Soybean Variety JS 97-52 in Kohima District of Nagaland, India
Authors: Shitiri Martina , Mezhatsu Ruokuo
Published: May 2024

Krishi Vigyan Kendra, Kohima District of Nagaland a conducted front-line demonstration with 10 beneficiaries in Tesophenyu village during the Kharif season of 2021-22 and 2022-23. Soybean variety JS 97-52 high yielding was selected for demonstration with all improved practices along with a control plot where farmer’s practices were carried out. The productivity of soyabean yield ranges from 1820-1749 kg/ha with mean yield of 1784.5kg/ha under the demonstrated plot. The mean yield increases from 44.06% during the year 2021-22 and 41.08% during the year 2022-23 over farmer’s practice indicating the existence of technology gap and extension gap. The technology index varied from 30.02 % to 27.3% which shows the feasibility and effective of the newly demonstrated under farmer’s field. The average benefit cost ratio of recommended practices was 3.73 and that of farmers practice was 2.89.
Proximate Analysis and Mineral Content of Wild Edible Mushrooms from Manipur, India
Authors: Khumlianlal Joshua , Huidrom Surmani , Sharma K. Chandradev , Thachunglura V. L. , Indira Sarangthem
Published: May 2024

Integrating mushrooms into diets supports sustainable development goals by increasing nutritional variety, lowering environmental impact compared to livestock cultivation, strengthening local economies, and improving food security. The present study aimed to assess the nutritional value and mineral content of five edible wild mushrooms belonging to the family Russulaceae. In Manipur, wild mushrooms are primarily gathered and highly valued as a traditional nutritious food among the rural poor. Despite this, their nutritional characteristics have not been adequately studied and documented. Lactifluus dwaliensis, Lactifluus volemus, Russula rosea, Russula nigricans, and Russula virescens collected from Churachandpur, Manipur were initially identified based on morphological and molecular analysis. The nutrient analysis of the wild edible mushrooms collected revealed that they are an excellent source of protein and carbohydrates, while containing low amounts of fat. These mushrooms also showed high levels of essential minerals that are essential for human nutrition and overall health.
STUDI TENTANG PELAKSANAAN KONSINYASI DALAM PENGADAAN TANAH UNTUK KEPENTINGAN UMUM DI PENGADILAN NEGERI BOYOLALI
Authors: Salsabila Nonik Eka , Harjono Harjono
Published: May 2024

Artikel ini mendeskripsikan dan mengkaji permasalahan bagaimana pelaksanaan konsinyasi dalam pengadaan tanah untuk kepentingan umum di Pengadilan Negeri Boyolali serta apa akibat hukum pelaksanaan konsinyasi dalam pengadaan tanah untuk kepentingan umum di Pengadilan Negeri Boyolali. Tujuan artikel ini adalah untuk mengetahui pelaksanaan konsinyasi dalam pengadaan tanah untuk kepentingan umum serta mengetahui apa akibat hukum dari pelaksanaan konsinyasi dalam pengadaan tanah untuk kepentingan umum. Temuan membuktikan bahwa pelaksanaan konsinyasi dalam pengadaan tanah untuk kepentingan umum di Pengadilan Negeri Boyolali didasarkan pada Undang-Undang Nomor 2 Tahun 2012, sedangkan prosedur konsinyasi di Pengadilan Negeri Boyolali dilakukan sesuai dengan PERMA RI No 2 Tahun 2021 jo. PERMA RI No 3 Tahun 2016. Akibat hukum dari adanya pengesahan konsinyasi oleh Pengadilan Negeri adalah lepasnya hak atas tanah menjadi tanah yang dikuasai langsung oleh negara.
Penentuan Keturunan dengan Menggunakan DNA pada Tulang Belulang Jenazah : Systematic Review
Authors: Agusti Herratri Wikan Nur
Published: May 2024

Pendahuluan : Mengetahui silsilah keluarga ataupun asal muasal seseorang merupakan salah satu sarana dalam identifikasi seseorang. Penggunaan DNA (Asam Deoksiribonukleat) untuk mengetahui asal muasal dari seseorang hingga dapat mengidentifikasi warna mata, warna kulit, hingga warna rambut dari sampel tulang sedang tren di Amerika Serikat dan Eropa. Di Indonesia proses identifikasi pada jenazah yang telah menulang masih kurang informatif. Pelaksanaan identifikasi DNA tidak banyak digunakan akibat biaya operasional yang tinggi dan membutuhkan database yang besar.
Tujuan : Menggali literatur tentang penggunaan DNA untuk identifikasi dan mencari garis keturunan.
Metode : Sistematik review data tahun 2017 - 2022 pada pencarian pubmed. Ekstraksi data dengan kunci pencarian berupa ‘geneology remains’ ‘human’ dan ‘DNA’. Peninjauan bahasan hanya khusus jurnal yang termasuk dalam free fulltext.
Hasil : Jurnal yang sesuai dengan kunci pencarian sebanyak 22 jurnal diantaranya jurnal free fulltext dan menggunakan subyek manusia hanya 9 jurnal. Jurnal tersebut membahas mengenai penyakit keturunan, database DNA, laporan kasus, dan membahas metode penelitian DNA itu sendiri. Mayoritas pengukuran dengan menggunakan kromosom Y untuk mengukur keturunan, namun dapat juga menggunakan kromosom lain.
Kesimpulan : Pemeriksaan DNA dapat digunakan untuk mengukur keturunan dengan sangat detail terutama pada sampel tulang namun masih banyak limitasi sehingga aplikasi di masyarakat menjadi sangat kurang.
Key changes in the salary structure and the procedure for making special social payments.
Authors:   Russian Research Institute of Health, Starodubov V.I. , Kadyrov F.N. ,   Russian Research Institute of Health, Obukhova O.V. ,   Russian Research Institute of Health, Endovitskaya Yu.V. ,   Russian Research Institute of Health, Chililov A.M. ,   A.V. Vishnevsky National Medical Research Center of Surgery
Published: May 2024

The purpose of this study is to analyze the consequences of government decisions on remuneration and implementation of special social benefits. The key provisions of the new regulatory legal acts in this area are considered. An assessment of the upcoming changes in the wage system is given, in terms of the possible impact on the stimulating qualities of the wage system. Recommendations have been developed on possible options for actions by public authorities of the subjects of the Russian Federation and state (municipal) institutions in order to achieve the established share of salaries in the amount of 50% of the total salary. The impact of the changes under consideration on the preparation of the transition to a new wage system in healthcare is assessed.
The Impact of Patient Education on Inpatient Fall Risk
Authors: Modica Anthony , Bitterman Adam D.
Published: May 2024

» Patient falls in the hospital are one of the leading sentinel events identified by the Joint Commission.

» Hospital falls affect orthopaedic patients of older age at higher rates, and up to 34% of falls lead to injury such as fractures.

» Patients often misperceive their fall risk, and modalities aimed at educating patients to address the incongruence between perceived and actual fall risk significantly reduce fall rate and injurious fall rate.

» Adequate communication with patients and their families with the goal of educating them may diminish the physical, psychological, and emotional detriment to orthopaedic patients.
Perception of Illness and Life Satisfaction Following a Week of Yoga Therapy in Patients with Chronic Diseases
Authors:   Department of Yoga Research, Patanjali Research Foundation, Haridwar 249405, Uttarakhand, India., Telles Shirley , Katare Neerja , Sharma Sachin Kumar , Gupta Aditi ,   Department of Yoga Research, Patanjali Research Foundation, Haridwar 249405, Uttarakhand, India.
Published: May 2024

Background: The present study aimed to determine (i) the effect of a residential yoga therapy program on the perception of illness and life satisfaction in patients with non-communicable diseases and (ii) the relationship between perception of illness and life satisfaction in the study population. Methods: The patients were recruited from a yoga therapy center. All the patients were assessed for life satisfaction and perception of illness at the baseline and after one week of yoga therapy. Data obtained at baseline after 7 days was compared with paired t-test. Association between perception of illness and life satisfaction were determined using Pearson correlation. Results: There was significant negative relations between of life satisfaction and perception of illness, the both perception of illness and life satisfaction improve after 7 days of yoga therapy. Conclusion: Yoga therapy may be useful in improving life satisfaction and to perception of illness. This will facilitate a positive outcome and the patients tends to get maximum benefit of the therapy.
Extra-Nasopharyngeal Angiofibroma of the Hard Palate: An Uncommon Case Reported in an Adult Female
Authors:   Department of Oral Pathology & Microbiology and Forensic Odontology, GITAM Dental College & Hospital, Vishakhapatnam 530045, Andhra Pradesh, India., Singhal Ishita , Sankar Mamidi , Majumdar, Sumit , Ogirala Smyrna ,   Department of Biomedical, Surgical & Dental Sciences, Università Degli Studi di Milano, Milan, Italy,
Published: May 2024

Angiofibromas, accounting for a mere 0.05-0.5% of all head and neck neoplasms, are uncommon benign tumors originating from connective tissue and characterized by a high vascularity and local aggressiveness. Juvenile Nasopharyngeal Angiofibroma, extensively discussed in medical literature, represents the most common subtype and is typically found in the nasopharynx. Extra-nasopharyngeal angiofibromas, which occur in locations outside the nasopharynx, are quite rare, with the maxillary sinus being the most typical site according to existing research. This paper presents a unique case of Extra-nasopharyngeal Angiofibroma affecting the hard palate in an adult female. This case is noteworthy due to the tumor's anterior hard palate location, its occurrence in a female patient, and its clinical resemblance to a fibroma, making it the first reported case of its kind.
A Case report of Anemia Masquerading as Myocardial infarction Unveiling Diagnostic Dilemmas in the Emergency Department
Authors:   Department of Emergency Medicine, MAX Superspeciality Hospital, Vaishali, Ghaziabad 201010, Uttar Pradesh, India., Shishodia Shakti , B Gokul , Shegal Pallavi , Nair Dheeraj B ,   Department of Emergency Medicine, MAX Superspeciality Hospital, Vaishali, Ghaziabad 201010, Uttar Pradesh, India.
Published: May 2024

44 Year old Male Presented to Er with Breathing difficulty, Sweating, altered mental status. Patient was tachypneoic and Hypotensive and drowsy, Responding to painful stimuli. Patient blood gas analysis revealed severe metabolic and lactic acidosis and patient was intubated and resuscitated aggresively in Er. Ecg was obtained which showed global St/ T changes and hence cath lab was activated and Echo was done in Er which didn’t reveal any wall motion abnormalities. Lab reports revealed severe anemia and patient was transfused with prbcs and patient condition improved and Ecg changes were also reverted.
Comparing Library Science Education in India and Australia: Issues, Challenges and Growth Strategies for India
Authors:   , Department of Library and Information Science, Aligarh Muslim University, Aligarh 202001, Uttar Pradesh,, Ansari Mehtab Alam , Munshi Shamim Aktar ,   Ananda Chandra College, Jalpaiguri 735101, West Bengal, India.
Published: May 2024

Library education in India and Australia has developed independently with distinct features. This paper provides brief profile of the history of India and Australia followed by discussion of the evolution of LIS education in both the countries. The paper is a comparative study. It discusses the different issues and challenges encountered in LIS education in India and Australia. This comparison is an attempt to critique improvements that must be made to LIS education in India to ensure on going sustainability and relevance. In India, the growth of library schools; dated LIS curricula; increasing student faculty ratios; accreditation; and the employability of graduates are some key issues. In Australia challenges include the broad employment landscape; mismatch between pay and qualifications; decreasing numbers of educators; existence of a multi-tiered system; LIS students and institutional affiliations. A number of strategies have been used in both India and Australia to enhance LIS education. Using LIS education in Australia as a benchmark the authors show how LIS education in India can be enhanced and improved.
Newspaper Reading Habit of College Students: A Study of Guru Nanak College Budhlada
Authors:   Bhai Gurdas Library, Guru Nanak Dev University, Amritsar, Punjab 143005, India., Ghuman Surinder Singh , Maan Inderjeet Singh ,   Bhai Gurdas Library, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
Published: May 2024

The present study was done to know the newspaper reading habits of the students studying at the Guru Nanak College Budhlada which is situated in the rural belt of Punjab. Structured questionnaire was prepared and distributed to one hundred students of college through google form. Study was conducted to know the various preferences regarding newspaper reading. Data received from the google form was presented through pie charts and graphs. Study found that most of the respondents daily read newspaper for current affairs purpose and education is their favorite section. Students prefer hard copy of the newspaper in Punjabi language. College students find availability of news on other media and busy schedule as major hindrances to reading newspaper.
Information Literacy Skills among PG Students and Researchers in Accessing the Resources
Authors:   Assistant Librarian, Bangalore University Library, Bang, N Padmavathi , J Nayana ,   Senior Library Cum Information Assistant, Centre for Nano & Soft Matter Sciences, Bengaluru, Karnataka 562162, India.
Published: May 2024

Information Literacy is essential for each individual to obtain the required Information from the vast availability of resources. With Information Literacy, one can identify, evaluate, apply and acknowledge the source of Information. Information Literate evolution plays a important function in the growth of the Nation. Significant steps need to be taken to transform the young Indians into Information literate Indians. The lifelong learning process should be an essential component of the Higher education system. The present study attempted to understand the level of understanding of information sources available in the university, recognize their level of digital Literacy, and find out their diverse search strategies.
What Environmentalists want to Cite: A Bibliometric Study based on Environmental Conservation Journal
Authors:   Information Scientist, Gurukul Kangri (Deemed to be University) Haridwar 249404, Uttarakhand, India., Dhiman Anil Kumar , Kaushik Sachin Kumar ,   Information Scientist, Gurukul Kangri (Deemed to be University) Haridwar 249404, Uttarakhand, India.
Published: May 2024

The current bibliometric study is focussed on an examination of 255 articles/research papers that were published in the Environmental Conservation Journal, a venerable journal in the environmental area by taking into consideration its last five volumes from 2016 to 2020. In addition to discussing the average length of an article and the number of references per article, the study also covers the year and volume-wise number of articles, authorship patterns, most productive authors of the journal, state-wise contribution of the articles, and foreign contribution to the journal.
Marketing Strategy for Information Products and Services in Library: In view of the Ranganathan’s five Laws of Library and Information Science
Authors:   Department of Library and Information Science, Dr. Harusingh Gour Vishwavidyalaya (A Central University), Sagar, Madhya Pradesh  470003, India, Kumar Mahendra
Published: May 2024

he paper emphasises the need of information products and services and marketing strategies five laws of LIS have been amended to match the marketing of Library Services.
Relation to Social Determinants in Cardiovascular Disease Risk Profile: An Alarming View
Authors:   Hindu Mission College of Nursing, West Tambaram, Chennai 600045, Tamil Nadu, India., S. Rajathi , Hemamalini M. , Sriramalakshmi C. ,   Hindu Mission College of Nursing, West Tambaram, Chennai 600045, Tamil Nadu, India.
Published: May 2024

Globally, Cardiovascular disease (CVD) is one of the major reasons for mortality. Cardiovascular health in children and adults is profoundly affected by the milieu of early life. Currently, CVD is taking a different turn based on social antecedents like poverty, the difference in socio-economic status, gender inequality, being a fluid person, and work life in harmony increases the risk of getting into CVD, that too in vulnerable populations like racial and ethnic minorities, women, the elderly, the chronically ill individual with disabilities, lesbian, gay, bisexual, transgender, queer and racism play a significant role in conditioning disease burden and modulating outcomes of CVD. People with low socioeconomic status increase the hypothalamic pituitary adrenal axis (HPA axis) in the body, due to stress response, and at the end, increased cortisol and sympathetic nervous system activity lead to decreased angiogenic activity. People from low socioeconomic backgrounds, loneliness, and social isolation in many cases like racism, ethnic minorities, children, women, elderly, and chronically ill individuals with disabilities, are exposed to constant stress because of a lack of their fundamental needs. The wider introduction of universal screening for social factors that impact cardiac health will help to identify children and families at risk. Hence,aggressive screening tests beginning at an early age will be beneficial for early detection and treatment. Healthcare professionals need to pay attention to promotinghealth education and awareness aids to decrease CVD-associated mortality.
WITHDRAWN: The effect of a group counselling program to reduce the level of future anxiety and psychological stress among gifted students
Authors: Mahasneh Ahmad M. , Mhaiat Fatin A. , Abood Mohammad H.
Published: May 2024

The full text of this preprint has been withdrawn by the authors while they make corrections to the work. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
WITHDRAWN: Mathematical Analysis of the Dynamics of Lumpy Skin Epidemics within Delay Differential Equations
Authors: Fadhal Emad , Raza Ali , Baleanu Dumitru , Naveed Muhammad , Defterli Ozlem , Rafiq Muhammad , Ahmed Nauman
Published: May 2024

The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Pseudomonas putida A (ATCC 12633) produces polyhydroxyalkanoates as by products during the degradation of synthetic ammonium compounds commonly found in wastewater
Authors: Heredia Romina , Liaudat Ana Cecilia , Liffourrena Andrés Sebastián , Boeris Paola Sabrina
Published: May 2024

Purpose
Assess to production of polyhydroxyalkanoates (PHAs) by Pseudomonas putida A (ATCC 12633), when it degraded the pollutant tetradecyltrimethylammonium bromide (TTAB), a synthetic quaternary ammonium compound (QAC) commonly present in wastewater. Our ultimate purpose is to develop sustainable strategies to convert waste products into high value-added products, like PHAs.
Methods
P. putida was cultivated in the presence of preferred carbon and nitrogen sources or solely with TTAB as a carbon and nitrogen source. PHAs presence was examined through TEM, fluorescence microscopy, and flow cytometry and quantified using gravimetric techniques. Constituent monomers were identified via GC-MS. qRT-PCR assays were performed to pinpoint genes implicated in PHAs synthesis and degradation.
Results
Maximum accumulation (52% of the dry cell weight) was achieved at 48 h of cultivation with TTAB. The accumulated PHAs were medium-chain-length: methyl 3-hydroxy-decanoate (65.1%), methyl 3-hydroxy-octanoate (11.9%), methyl 3-hydroxy-dodecanoate (8.4%), and methyl 3-hydroxy-tetradecanoate (14.6%). Regarding the relative expression levels of genes involved in PHA polymerization/depolymerization, those of phaC1 peaked at 48 h, but those of phaZ were detectable only after 72 h.
Conclusion
P. putida’s ability to remove a QAC and produce PHAs as a result makes it a promising candidate for the biotechnological conversion of waste into valuable biopolymers.
Prevalence of molar incisor hypomineralization and demands for treatment according to the severity of its clinical manifestation.
Authors: Guerra Bianca Mattos Santos , Jorge Roberta Costa , Reis Patrícia Papoula Gorni dos , Machado Gabriella de Freitas , Fidalgo Tatiana Kelly da Silva , Soviero Vera Mendes
Published: May 2024

Aim Assess the prevalence and severity of molar incisor hypomineralization (MIH) in children 6- to 12-year-old and correlate MIH severity with the complexity of treatment demands.Materials and methods Between March and October 2023, 2,136 children were screened for MIH by two calibrated examiners. MIH severity was categorized in mild, moderate, severe, and very severe. Treatment requirements were categorized in basic, intermediate, and complex.Results The prevalence of MIH was 15.5% (n = 331). Among the 126 children with atypical caries/restoration, cusp involvement was observed in 60.3%, endodontic treatment was necessary in 24.6%, only 15% needed restorations limited to two tooth surfaces with no cusp involvement. Extraction due to MIH was observed in 2.7%.Conclusion The prevalence of MIH was in accordance with previous literature. Whenever a restorative treatment was necessary, it often involved multiple tooth surfaces and cusps. The complexity of treatment demands increased with age. It is necessary that oral health policymakers pay special attention to children with MIH to ensure appropriate treatment.
Sustainable Organizational Adaptation: Private Higher Education's Response to COVID-19 Challenges
Authors: Ramachandaran Sharmila , Badwy Hanan Eid
Published: May 2024

This study analyses organizational development and change approaches in private higher education institutions (HEIs) in response to COVID-19 encounters. Through case studies and stakeholder interviews, it pinpoints four key factors: organizational adaptation, faculty role redefinition, digital transformation, and sustainability. Remarkably, sustainability emerges as a critical aspect, integrating environmental, social, and economic considerations into strategies. These include lowering environmental impact, fostering social equity, and safeguarding economic resilience. The study highlights the importance of understanding HEIs' responses to crises and their effect on organizational development. The understandings of the study can inform policymakers and administrators, enhancing crisis management and organizational strategies for greater resilience. By assimilating sustainability into these strategies, HEIs can align with global agendas like the UN Sustainable Development Goals, contributing to a more sustainable future for higher education.
Effects of heat stress on production performance and protein metabolism of skeletal muscle in meat rabbits
Authors: Liu Gongyan , Liu Ce , Sun Haitao , Bai Liya , Yang Liping , Zhang Yin , Gao Shuxia
Published: May 2024

In modern animal husbandry production, rabbits are readily affected by heat stress in summer. A critical issue in rabbit production is to determine the mechanism by which heat stress prevents muscle growth. The purpose of this experiment was to study the effects of heat stress on performance and protein metabolism of skeletal muscle in meat rabbits. A total of 160 New Zealand White rabbits aged 80 days with mean initial body weights of 2359 ± 200 g were randomly divided into the control group and heat stress group. The experiment duration was 20 days. Heat stress treatment reduced the growth performance and slaughter performance of the rabbits (P < 0.05), and increased muscle yellowness (b*, P < 0.05). In addition, heat stress treatment increased the concentrations of leptin, cholesterol, high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL) cholesterol in serum (P < 0.05), and decreased the serum total protein and immunoglobulin (IgG, IgM, and IgA) contents of rabbits. Under the criteria fold-change ≥ 1.20 or ≤ 0.84 and P-value ≤ 0.05, seven up-regulated proteins and 122 down-regulated proteins were screened. A gene ontology (GO) enrichment analysis of the differentially expressed proteins was performed. The most enriched specific GO terms among the differential proteins were response to stress, extracellular region, and protein binding in the biological process, cellular component, and molecular function categories, respectively, and the most enriched pathway was PI3K/Akt signalling pathway. Heat stress reduced the carcass yield of growing meat rabbits, changed the physical characteristics of the skeletal muscle, influenced protein metabolism by changing blood indices potentially through the PI3K/Akt signalling pathway.
Peripherally inserted central venous catheter in outpatient antibiotic spinal infection treatment is safe, effective and leads to significant reduction in healthcare expenses
Authors: Bonk Maximilian-Niklas , Sommer Björn , Krauß Philipp E. , Maurer Christoph , Simon Valeska , Berlis Ansgar , Hofmann Reinhard , Shiban Ehab
Published: May 2024

Introduction: Prolonged antibiotic therapy is often recommended for the treatment of spinal infections. This study aimed to evaluate the efficacy and safety of outpatient intravenous (IV) antibiotic therapy for spinal neurosurgery patients with spondylodiscitis.
Methods: We carried out a retrospective study involving 67 patients who were administered peripherally inserted central catheter (PICC) for IV antibiotic treatment from January 2020 to December 2022. We assessed patient data concerning infections and neurosurgical concerns. Each patient underwent a minimum of 6 weeks of IV antibiotics, both as inpatients and outpatients.
Results: The study included 67 patients with a median age of 61 years (SD +/- 14.18 years), with approximately 44% being female. The average hospital stay for inpatient treatment was 20 days (SD +/- 8.8 days). Subsequent outpatient antibiotic therapy lasted an average of 70.32 days (SD +/- 18.24 days), with outpatient IV therapy accounting for 44.74 days (SD +/- 9.15 days). The most common pathogens identified were Staphylococcus epidermidis and methicillin-sensitive Staphylococcus aureus. Microbiological analysis did not detect any pathogens in 18% of patients. Radiographic and laboratory evidence of spondylodiscitis was absent in 99% of patients during the final follow-up. No catheter-related complications occurred.
Conclusion: Outpatient IV antibiotic therapy using a PICC line catheter is a safe and effective treatment option for spinal infections, especially in elderly patients.
Assessment of National Immunization Technical Advisory Group (NITAG) functionality in selected Eastern Mediterranean countries
Authors: Parwiz Sardar , Al-gunaid Magid , Bashour Hyam , Khader Yousef , Saeed Khwaja Mir Islam , Lami Faris , Daoud Leen , Majeed Zeina Abdel , Hussain Zakir
Published: May 2024

National Immunization Technical Advisory Groups (NITAGs) are independent advisory committees providing evidence-based recommendations for decision-making on new vaccine introduction and immunization policy. This report, addresses issues of the functionality of the NITAGs in the selected EMR countries, building the capacity of NITAG to improve the functionality of NITAG in the targeted counties as part of EMPHNET support to health systems strengthening in the region. This assessment was conducted in 13 EMR countries Afghanistan, Algeria, Iraq, Jordan, Libya, Morocco, Oman, Qatar, Tunisia, Saudi Arabia, Sudan, Syria, and Yemen. The target population was NITAG chairperson and NITAG technical secretary (NEPI). the assessment tool was digitalized using a KoBo toolbox. Following the data analysis, a five-day inter-country workshop was conducted to provide a facility to the countries under review to develop country improvement plans to accomplish the NITAG functionality in the region. According to the findings, all of the under-review countries have active NITAG with the written terms of reference. For 41% of the respondents, the role of NITAG was not clear and they mentioned that the NITAG directly makes the policy. Based on the findings 36% of the countries don’t have SOPs for their NITAGs. Regarding the membership, all the countries mentioned that their NITAG consists of multidisciplinary experts. However, 46% of the countries faced a shortage of experts. Likewise, 69% of the countries reported high turnover of the NITAG members which negatively affects the functionality of NITAG. Based on the findings the main challenges to the NITAG included staff turnover, shortage of technical capacity, and the load on human resources. Some highlighted political or personal biases. Financial challenges were also noted in all countries except high-income countries. Only 24.5% of the participants mentioned that they documented the NITAG recommendations separately from the meeting minutes. Likewise, 59% of the participants mentioned that the NITAG uses a standardized and systematic method of searching for, reviewing, and synthesizing relevant evidence based on a PICO-like framework for the policy question. The findings of the regional assessment clearly showed that major gaps need to be tackled to ensure the functionality of NITAG. Clear TOR, SOPs, the clear and well-defined role of secretariats, and ensuring the systematic provision of the recommendation for policymakers.
Participant-reported personal utility of genetic testing for Parkinson’s disease and interest in clinical trial participation
Authors: Verbrugge Jennifer , Oas Hannah , Cook Lola , Schwantes-An Tae-Hwi , Walsh Laurence , Wills Anne , Mata Ignacio , Nance Martha , Beck James , Naito Anna , Marder Karen , Alcalay Roy
Published: May 2024

Genetic testing for Parkinson’s disease (PD) is infrequently performed in clinical settings due to providers’ perception of low utility, yet patients may find the results useful. We investigated the reported personal utility of genetic testing for PD as part of the PD GENEration study, in which genetic testing and counseling are provided at no cost to participants. We also investigated how genetic test results may encourage enrollment in additional research studies. In the PD GENEration study, PwP underwent genetic testing for seven genes associated with PD and received genetic counseling to discuss results. Participants (n=972) completed a survey examining the perceived personal utility of their results and interest in participating in additional research studies. Most participants found their genetic test results useful in several ways, including satisfying their curiosity (81% of participants), feeling good about helping the medical community (80%), and having information to share with family (77%). There were no significant differences in perceived personal utility between gene-positive and gene-negative participants. Forty-five percent of all participants expressed interest in participating in additional research studies and 16% of all participants confirmed enrollment in other studies. Our results suggest that participants find personal utility in genetic testing regardless of results. Although participants may be interested in enrolling in additional research, they may need more support and resources to do so.
Study of Neutrophil CD64 and HLA-DR as Early Detection Markers of Sepsis in Hepatic ICU Patients
Authors: Elsabaawy Maha , Elazab Gasser , Khalil Fatma , Alhajajy Fatma , Awadein Mohamed , Elmaksoud Fatma Abd , Abdelkareem Mervat
Published: May 2024

Background
Sepsis is the commonest cause of death in liver cirrhosis patients.
Aim
Evaluation of neutrophil and monocyte CD64 and HLA-DR as early biomarkers predicting sepsis in liver cirrhosis.
Methods
This case-control study involved 70 cirrhotic patients (35 with sepsis and 35 without) and 30 healthy individuals. Laboratory studies were performed, including CD64 and HLA-DR using flow cytometry along with sepsis index (SI).
Results
Patients were mainly males (80%), aged 62.17 and 64.69 years respectively. Mono CD64% at Cut-off: >62.9 showed AUC: 0.676, Sensitivity: 80.0%, Specificity: 60.0%, PPV: 66.7%, and NPV: 75.0%. while Mono CD64 MFI at Cut-off > 9.5 shoed AUC: 0.659, Sensitivity: 94.29%, Specificity: 37.14%, PPV: 60.0%, and NPV: 86.7%. Combinations of CRP + Lactate + Mono CD64%+ Mono CD64 MFI revealed AUC: 0.929, Sensitivity: 82.86%, Specificity: 91.43%, PPV: 90.6%, and NPV: 84.2% Mono CD64% and CD64 MFI (0.003; 0.03) respectively. Regression analysis defined Mono CD64% >62.9 (OR 6), Mono CD64 MFI > 9.5 (OR 9.75), CRP > 32.4 (OR 13.5), lactate > 1.93 (OR 12.08), and ALBI score > 0.01 (OR 6) all as factors affecting early sepsis in cirrhosis.
Conclusion
Mono CD64% and Mono CD64 MFI proved efficacy as early septic biomarkers with higher efficacy when combined with traditional inflammatory markers in liver cirrhosis patients.
Pregnancy outcomes in correlation with placental histopathology in pregnancies complicated by fetal growth restriction with vs. without reduced fetal movements
Authors: Mor Liat , RABINOVITCH TAMAR , Schreiber Letizia , Paz Yael Ganor , Barda Giulia , Kleiner Ilia , Weiner Eran , Levy Michal
Published: May 2024

Purpose: Fetal movements are crucial indicators of fetal well-being, with reduced fetal movements (RFM) suggesting potential fetal compromise. Fetal growth restriction (FGR), often linked to placental insufficiency, is a major cause of perinatal morbidity and mortality. This study aimed to investigate the neonatal, labor, and placental outcomes of FGR pregnancies with and without RFM at term.
Methods: In this retrospective study, data from all term, singleton deliveries with FGR and concomitant RFM were obtained and compared to an equal control group of FGR without RFM. Maternal characteristics, pregnancy and neonatal outcomes, and placental histology were compared. The primary outcome was a composite of adverse neonatal outcomes. A multivariable regression analysis was performed to identify independent associations with adverse neonatal outcomes.
Results: During the study period, 250 FGR neonates with concomitant RFM and an equal control group were identified. The groups did not differ in maternal demographics aside from significantly higher rates of maternal smoking in the RFM group (p<0.001). Polyhydramnios and oligohydramnios (p=0.032 and p=0.007, respectively) and meconium-stained amniotic fluid (p<0.001) were more prevalent in the FGR+RFM group. Additionally, the RFM group showed higher rates of adverse neonatal outcomes despite having larger neonates (p=0.047 and p<0.001, respectively). No significant differences were observed in placental findings. Logistic regression identified RFM as an independent predictor of adverse neonatal outcomes (aOR 2.45, 95%CI 1.27-4.73, p=0.008).
Conclusion: Reduced fetal movements are significant and independent predictors of worse neonatal outcomes in FGR pregnancies, suggesting an additional acute insult on top of underlying placental insufficiency.
Non-linear dose-response relationship between serum albumin and acute kidney injury in sepsis patients: a cohort study
Authors: Liang Xiaomin , Hu Haofei , Chen Xinglin , Zhou Yan , Li Guiyun , Wen Sha , Mei Jiang , Zhang Lin , Gui Shuiqing , Li Ying
Published: May 2024

Background
The role of serum albumin on acute kidney injury (AKI) remains controversial. Additionally, research on this relationship in sepsis patients is sparse. Therefore, this research aimed to investigate the relationship between serum albumin level and probability of AKI in patients with sepsis.
Methods
This study was a retrospective cohort analysis of sepsis patients across the United States between 2014 and 2015 in the eICU Collaborative Research Database (eICU-CRD). To estimate the role of albumin on AKI by univariate, multivariate logistic regression and smooth curve fitting analysis.
Results
Among 5459 patients with sepsis, 32.94% (1798 patients) developed AKI. The results of the multivariate logistic regression analysis indicated that the albumin and AKI were negatively correlated (adjusted OR = 0.87, 95% CI 0.77–0.98, P = 0.0199). Moreover, a nonlinear relationship was observed between albumin level and probability of AKI with a inflection point at 2.1 g/dL. For albumin level < 2.1g/dL, each unit increase in serum albumin reduced the probability of AKI by 39% (adjusted OR = 0.61; 95% CI 0.44–0.85; P = 0.0032). However, for albumin levels above 2.1 g/dL, there was no significant association with the probability of AKI (adjusted OR = 0.99, 95% CI 0.83–1.16; P = 0.8691).
Conclusion
Serum albumin level below 2.1g/dL was defined as a risk factor for AKI in sepsis patients.
The application effect of the segmented teaching method in training medical students on clinical practice skills
Authors: Liu Chao , Ren Mei , Luo Chaoen , Asfandyar Khan , Dai Huijie , Yang Jinxin , Lei Zhengwen
Published: May 2024

The surgical clinical practice skill training is an important part of medical undergraduate education. Surgical skills are complex and difficult to master. However, the traditional teaching method has some disadvantages, for example, the form is stereotyped and monotonous, the content is dull and boring. We innovatively put forward the segmented teaching method. In the segmented teaching method, the theoretical knowledge and operation steps were divided into three stages: pre-operation, during operation, and post-operation. The theoretical knowledge was explained in stages, the operation was segmented, the training was segmented, the timing was segmented, the comment was segmented, and the overall reinforcement was finally achieved. The results of debridement and suture practice training show that this teaching model can improve the clinical practice ability of medical students, and can achieve good training effect in the surgical clinical practice skill training.