bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Comparison of the Hydraulic Performance and Pressure Pulsation Characteristics of a Shaft Front-Positioned and a Shaft Rear-Positioned Tubular Pump Devices
Authors: Ji Dongtao , Lu Weigang , Lu Linguang , Xu Lei , Liu Jun , Shi Wei , Huang Guohao
Published: December 2021

The shaft front-positioned tubular pump device has been widely used in practical engineering, but the shaft rear-positioned pump device is rarely used due to its low efficiency. To investigate the effect of the shaft position on the performance of a tubular pump device, the optimized shaft front-positioned and shaft rear-positioned pump devices were compared and studied. Both tubular pump devices adopt a TJ04-ZL-06 pump model. Three dimensional steady and unsteady numerical simulations combined with model tests were used to compare the difference of two pump devices. Meanwhile, three groups of pressure monitoring points were set at different positions of the pump device to collect pressure information and pressure pulsation was analyzed. The results show that, the highest efficiency of the shaft front and rear positioned pump device are 81.78% and 80.26%, respectively. The hydraulic performance of the two inlet passages is excellent, and the hydraulic loss is close to each other. Therefore, the hydraulic performance of the pump device depends mainly on the hydraulic performance of the outlet passage. The shaft is set in the outlet passage, which will increase the hydraulic loss and reduce efficiency. Under design conditions, the pressure pulsation amplitude at the impeller inlet is the largest, and the pressure pulsation amplitude increases from the hub to the shroud. The pressure pulsation amplitude of the shaft rear-positioned pump device is larger than that of the shaft front-positioned pump device. The pressure pulsation at the impeller inlet and outlet is greatly affected by the number of blades, and the main frequency is three times the RF. This study can provide practical and effective guidance for the design and optimization of the shaft front-positioned and rear-positioned tubular pump devices, which has theoretical value and application value.
Methods for Manipulating Cryptococcus Spores
Authors: Frerichs Anna B. , Huang Mingwei , Ortiz Sébastien C. , Hull Christina M.
Published: December 2021

Spores are essential for the long-term survival of many diverse organisms, due to their roles in reproduction and stress resistance. In the environmental human fungal pathogen, Cryptococcus, basidiospores are robust cells with the ability to cause disease in animal models of infection. Here we describe methods for producing and purifying Cryptococcus basidiospores in quantities sufficient for large-scale analyses. The production of high numbers of pure spores has facilitated the development of new assays, including quantitative germination assays, and enabled transcriptomic, proteomic, and virulence studies, leading to discoveries of behaviors and properties unique to spores and spore-mediated disease.
Associations between the Levels of Estradiol-, Progesterone-, and Testosterone-Sensitive MiRNAs and Main Clinicopathologic Features of Breast Cancer
Authors: Kalinina Tatiana , Kononchuk Vladislav , Alekseenok Efim , Abdullin Grigory , Sidorov Sergey , Ovchinnikov Vladimir , Gulyaeva Lyudmila
Published: December 2021

Despite the existing advances in the diagnosis and treatment of breast cancer (BC), the search for markers associated with the clinicopathological features of BC is still in demand. MiRNAs (miRs) have potential as markers, since a change in the miRNA expression profile accompanies the initiation and progression of malignant diseases. The receptors for estrogen, androgen, and progesterone (ER, AR, and PR) play an important role in breast carcinogenesis. Therefore, to search for miRNAs that may function as markers in BC, using bioinformatic analysis and the literature data, we selected 13 miRNAs whose promoter regions contain binding sites for ER or AR, or putative binding sites for ER, AR, and PR. We quantified their expression in MCF-7 cells treated with estradiol, progesterone, or testosterone. The levels of miRNAs sensitive to one or more of these hormones were quantified in BC samples (n = 196). We discovered that high expression levels of miR-190b in breast tumor tissue indicate a positive ER status, and miR-423 and miR-200b levels differ between patients with and without HER2 amplification. The miR-193b, -423, -190a, -324, and -200b levels were associated with tumor size or lymph node status in BC patients, but the presence of these associations depended on the status and expression level of ER, PR, HER2, and Ki-67. We also found that miR-21 expression depends on HER2 expression in ER- and/or PR-positive BC. The levels of miRNA were significantly different between HER2 0 and HER2 1+ tumors (p = 0.027), and between HER2 0 and HER2 2+, 3+ tumors (p = 0.005).
Comparison of Bacterial Community in Paddy Soil after Short-Term Application of Pig Manure and the Corresponding Organic Fertilizer
Authors: Wang Min , Sha Chenyan , Wu Jianqiang , Li Peng , Tan Juan , Huang Shenfa
Published: December 2021

Agricultural fertilization significantly affects nutrients cycling in paddy soils. However, there are few systematic studies on the response of the application of livestock manure and corresponding organic fertilizer resulting in the microorganism and its nutrients entering farmland. Short-term application of pig manure and its corresponding organic fertilizer on the microorganisms of paddy fields were investigated. High-throughput sequencing technology was conducted to comprehensively study the microbial community composition and structure. Our results showed that organic fertilizer effectively enhanced nutrient substances such as nitrogen, phosphorus, and potassium at ripening stage and reduced the pH of soil, benefitting the soil fertility and the growth of rice. Though application of pig manure maintained higher microbial diversity, it increased the relative abundance of several pathogenic bacteria, which could threaten the soil health of the paddy fields. A large number of pathogenic bacteria can be reduced through composting pig manure to organic fertilizer. Nitrifying and denitrifying bacteria such as Anaerolineaceae, Pseudarthrobacter, Bacillus, and Nitrospira in the paddy soil were significantly promoted. The heavy metals such as Pb, Cr, and Cd, and nutrient substances such as phosphorus, as well as temperature, have important influences on the microbial compositions in ripening stage. The correlation analysis revealed more correlation efficiencies were observed with manure application, especially with the application of organic fertilizer. This study will provide a theoretical basis for improving land productivity and sustainable development in paddy fields.
Spatio-Temporal Pattern and Influence Mechanism of Cultivated Land System Resilience: Case from China
Authors: Lyu Xiao , Wang Yanan , Niu Shandong , Peng Wenlong
Published: December 2021

The study of cultivated land systems from the perspective of resilience is of great significance for the innovation of the research paradigm of cultivated land use and the rational utilization and protection of cultivated land. This study aims to explain the theoretical connotations of cultivated land system resilience (CLSR), construct an evaluation system and zoning rules for CLSR, and take 30 provinces of China as case study areas to explore the influencing factors of CLSR, so as to provide a reliable governance plan for the sustainable development of cultivated land. The results show that: (1) CLSR refers to a sustainable development ability that CLS—by adjusting the structure and scale of internal elements—absorbs and adapts to internal and external disturbances and shocks to the maximum possible extent, abandons the original inapplicable state, creates a new recovery path, achieves a new balance, and avoids system recession. (2) The overall CLSR of the 30 provinces showed an upward trend, and the degree of polarization of the distribution pattern was gradually intensified and experienced a transition process from “leading by resource and ecological resilience—equilibrium of each resilience—leading by production and scale structural resilience”. (3) In the north, east, and south coastal areas of China, CLSR mainly consists of the major evolution areas and the stable development areas; the potential excitation areas of CLSR are mainly concentrated in the central and western regions of China; the CLSR-sensitive lag areas and degraded vulnerable areas are mainly distributed in the northwest and southwest of China. (4) Water resource endowment has a strong influence on CLSR, while social economy mainly influences CLSR through ‘economic foundation-superstructures’ and ‘economic development-factor agglomeration’. (5) According to the different CLSR zones, CLSR was strengthened mainly from the aspects of driving factor agglomeration, building factor free-flow systems, and multi-means support.
Spinal Cord Involvement in Adult Mitochondrial Diseases: A Cohort Study
Authors: Primiano Guido , Mariotti Paolo , Turrini Ida , Sancricca Cristina , Sabino Andrea , Torraco Alessandra , Carrozzo Rosalba , Servidei Serenella
Published: December 2021

The central nervous system is metabolically very demanding and consequently vulnerable to defects of the mitochondrial respiratory chain. While the clinical manifestations and the corresponding radiological findings of the brain involvement in mitochondrial diseases (e.g., stroke-like episodes, signal changes of the basal ganglia, cerebral and cerebellar atrophy) are well known, at present there are few data on the spinal-cord abnormalities in these pathologies, in particular in adult subjects. In this study, we present a cross-sectional cohort study on the prevalence and characterization of spinal-cord involvement in adult patients with genetically defined mitochondrial diseases.
Impact of COVID-19 on Infertility Treatments: Not Even a Global Pandemic Was Strong Enough to Hamper Successful Pregnancies
Authors: Rodríguez-Varela Cristina , Mariani Giulia , Dolz Pilar , García-Velasco Juan Antonio , Serra Vicente , Pellicer Antonio , Labarta Elena
Published: December 2021

The COVID-19 global pandemic has meant a sanitary and social threat at every level and it was not any different for the assisted reproduction industry. This retrospective two-arm study aims to describe its impact on infertility treatments performed in our clinics (IVI Spain, Rome, and Lisbon) regarding: (1) assessment of COVID-19 impact in the amount, type, and success of infertility treatments performed during 2020 compared to 2019; and (2) description of the psychological status of women who got pregnant during the first months of the pandemic and its correlation with their final pregnancy outcome. On the one hand, this pandemic has led to a significant reduction in the total number of treatments performed, even though the proportion of the different types was almost unaltered. Additionally, its impact on pregnancy rates was not clinically relevant. On the other hand, the psychological status of pregnant women did not seem to affect their final pregnancy outcome. These results suggest that, even in the event of a negatively affected psychological status in our study population, it was not translated into an impaired pregnancy outcome. Hence, the COVID-19 global pandemic, although devastating, might not have exerted a clinically relevant negative impact on the overall pregnancy outcome in our clinics.
Lung Microbiome in Critically Ill Patients
Authors: Fromentin Mélanie , Ricard Jean-Damien , Roux Damien
Published: December 2021

The historical hypothesis of sterility of the lungs was invalidated over a decade ago when studies demonstrated the existence of sparse but very diverse bacterial populations in the normal lung and the association between pulmonary dysbiosis and chronic respiratory diseases. Under mechanical ventilation, dysbiosis occurs rapidly with a gradual decline in diversity over time and the progressive predominance of a bacterial pathogen (mainly Proteobacteria) when lung infection occurs. During acute respiratory distress syndrome, an enrichment in bacteria of intestinal origin, mainly Enterobacteriaceae, is observed. However, the role of this dysbiosis in the pathogenesis of ventilator-associated pneumonia and acute respiratory distress syndrome is not yet fully understood. The lack of exploration of other microbial populations, viruses (eukaryotes and prokaryotes) and fungi is a key issue. Further analysis of the interaction between these microbial kingdoms and a better understanding of the host-microbiome interaction are necessary to fully elucidate the role of the microbiome in the pathogenicity of acute diseases. The validation of a consensual and robust methodology in order to make the comparison of the different studies relevant is also required. Filling these different gaps should help develop preventive and therapeutic strategies for both acute respiratory distress syndrome and ventilator-associated pneumonia.
Effect of Annealing on Microstructure and Corrosion Behavior of Interstitial Free Steel
Authors: He Qiongyao , Jiang Xiaojuan , Cai Pengzhan , Zhang Ling , Sun Tao , Yang Xiaokui , Zhou Kun , Zhang Lunwu
Published: December 2021

Interstitial free steels with various grain sizes and textures were prepared by cold-rolling followed by an annealing process. The effect of grain size, crystallographic orientations and stored energy on corrosion behavior of interstitial free steel was investigated. It was found that the deformed microstructure and dislocation boundaries were consumed by recrystallizing grains during annealing. The average grain size increase ranging from 0.61 μm to 11 μm and the volume fraction of recrystallized grains was about 96% after annealing for 64 h; meanwhile, the γ fiber was the dominated recrystallized texture component. The stored energy gradually decreased due to the reduction in dislocation density by annealing. The potentiodynamic polarization and Nyquist plots show that the corrosion potential exhibits a more positive shift and depressed capacitive semicircle radius increase with rising annealing time. The 64 h annealed specimens had the biggest depressed semicircle in the Nyquist plots and the highest positive corrosion potential, which indicates the enhancement of corrosion resistance. Such an improvement of corrosion resistance is attributed to the increase in the volume fraction of the γ fiber and decrease in the stored energy.
Predicting the Ultimate Axial Capacity of Uniaxially Loaded CFST Columns Using Multiphysics Artificial Intelligence
Authors: Khan Sangeen , Ali Khan Mohsin , Zafar Adeel , Javed Muhammad Faisal , Aslam Fahid , Musarat Muhammad Ali , Vatin Nikolai Ivanovich
Published: December 2021

The object of this research is concrete-filled steel tubes (CFST). The article aimed to develop a prediction Multiphysics model for the circular CFST column by using the Artificial Neural Network (ANN), the Adaptive Neuro-Fuzzy Inference System (ANFIS) and the Gene Expression Program (GEP). The database for this study contains 1667 datapoints in which 702 are short CFST columns and 965 are long CFST columns. The input parameters are the geometric dimensions of the structural elements of the column and the mechanical properties of materials. The target parameters are the bearing capacity of columns, which determines their life cycle. A Multiphysics model was developed, and various statistical checks were applied using the three artificial intelligence techniques mentioned above. Parametric and sensitivity analyses were also performed on both short and long GEP models. The overall performance of the GEP model was better than the ANN and ANFIS models, and the prediction values of the GEP model were near actual values. The PI of the predicted Nst by GEP, ANN and ANFIS for training are 0.0416, 0.1423, and 0.1016, respectively, and for Nlg these values are 0.1169, 0.2990 and 0.1542, respectively. Corresponding OF values are 0.2300, 0.1200, and 0.090 for Nst, and 0.1000, 0.2700, and 0.1500 for Nlg. The superiority of the GEP method to the other techniques can be seen from the fact that the GEP technique provides suitable connections based on practical experimental work and does not rely on prior solutions. It is concluded that the GEP model can be used to predict the bearing capacity of circular CFST columns to avoid any laborious and time-consuming experimental work. It is also recommended that further research should be performed on the data to develop a prediction equation using other techniques such as Random Forest Regression and Multi Expression Program.
Safety Control Architecture for Ventricular Assist Devices
Authors: Cavalheiro André C. M. , Santos Filho Diolino J. , Dias Jônatas C. , Andrade Aron J. P. , Cardoso José R. , Tsuzuki Marcos S. G.
Published: December 2021

In patients with severe heart disease, the implantation of a ventricular assist device (VAD) may be necessary, especially in patients with an indication for heart transplantation. For this, the Institute Dante Pazzanese of Cardiology (IDPC) has developed an implantable centrifugal blood pump that will be able to help a diseased human heart to maintain physiological blood flow and pressure. This device will be used as a totally or partially implantable VAD. Therefore, performance assurance and correct specification of the VAD are important factors in achieving a safe interaction between the device and the patient’s behavior or condition. Even with reliable devices, some failures may occur if the pumping control does not keep up with changes in the patient’s behavior or condition. If the VAD control system has no fault tolerance and no system dynamic adaptation that occurs according to changes in the patient’s cardiovascular system, a number of limitations can be observed in the results and effectiveness of these devices, especially in patients with acute comorbidities. This work proposes the application of a mechatronic approach to this class of devices based on advanced control, instrumentation, and automation techniques to define a method to develop a hierarchical supervisory control system capable of dynamically, automatically, and safely VAD control. For this methodology, concepts based on Bayesian networks (BN) were used to diagnose the patient’s cardiovascular system conditions, Petri nets (PN) to generate the VAD control algorithm, and safety instrumented systems to ensure the safety of the VAD system.
Heterogeneous Diffusion, Stability Analysis, and Solution Profiles for a MHD Darcy–Forchheimer Model
Authors: Díaz José Luis , Rahman Saeed , García-Haro Juan Miguel
Published: December 2021

In the presented analysis, a heterogeneous diffusion is introduced to a magnetohydrodynamics (MHD) Darcy–Forchheimer flow, leading to an extended Darcy–Forchheimer model. The introduction of a generalized diffusion was proposed by Cohen and Murray to study the energy gradients in spatial structures. In addition, Peletier and Troy, on one side, and Rottschäfer and Doelman, on the other side, have introduced a general diffusion (of a fourth-order spatial derivative) to study the oscillatory patterns close the critical points induced by the reaction term. In the presented study, analytical conceptions to a proposed problem with heterogeneous diffusions are introduced. First, the existence and uniqueness of solutions are provided. Afterwards, a stability study is presented aiming to characterize the asymptotic convergent condition for oscillatory patterns. Dedicated solution profiles are explored, making use of a Hamilton–Jacobi type of equation. The existence of oscillatory patterns may induce solutions to be negative, close to the null equilibrium; hence, a precise inner region of positive solutions is obtained.
Comparison of Spinal Cord Stimulation vs. Dorsal Root Ganglion Stimulation vs. Association of Both in Patients with Refractory Chronic Back and/or Lower Limb Neuropathic Pain: An International, Prospective, Randomized, Double-Blinded, Crossover Trial (BOOST-DRG Study)
Authors: Rigoard Philippe , Roulaud Manuel , Goudman Lisa , Adjali Nihel , Ounajim Amine , Voirin Jimmy , Perruchoud Christophe , Bouche Bénédicte , Page Philippe , Guillevin Rémy , Naudin Mathieu , Simoneau Martin , Lorgeoux Bertille , Baron Sandrine , Nivole Kevin , Many Mathilde , Maitre Iona , Rigoard Raphaël , David Romain , Moens Maarten , Billot Maxime
Published: December 2021

While spinal cord stimulation (SCS) is a well-established therapy to address refractory persistent spinal pain syndrome after spinal surgery (PSPS-T2), its lack of spatial selectivity and reported discomfort due to positional effects can be considered as significant limitations. As alternatives, new waveforms, such as burst stimulation and different spatial neural targets, such as dorsal root ganglion stimulation (DRGS), have shown promising results. Comparisons between DRGS and standard SCS, or their combination, have never been studied on the same patients. “BOOST DRG” is the first prospective, randomized, double-blinded, crossover study to compare SCS vs. DRGS vs. SCS+DRGS. Sixty-six PSPS-T2 patients will be recruited internationally in three centers. Before crossing over, patients will receive each stimulation modality for 1 month, using tonic conventional stimulation. After 3 months, stimulation will consist in switching to burst for 1 month, and patients will choose which modality/waveform they receive and will then be reassessed at 6 and 12 months. In addition to our primary outcome based on pain rating, this study is designed to assess quality of life, functional disability, psychological distress, pain surface coverage, global impression of change, medication quantification, adverse events, brain functional imaging and electroencephalography, with the objective being to provide a multidimensional insight based on composite pain assessment.
Solvent-Resistant UV-Cured Polysulfone Support Membranes Using a Green Solvent
Authors: Dedvukaj Angela , Van den Mooter Peter , Vankelecom Ivo F. J.
Published: December 2021

Solvent-resistant UV-cured supports consisting of a semi-interpenetrating network of polysulfone (PSf) and cross-linked poly-acrylate were successfully synthesized for the first time using an alternative, non-reprotoxic, and biodegradable solvent. Tamisolve® NxG is a high-boiling, dipolar aprotic solvent with solubility parameters similar to those of dimethylformamide (DMF) and N-methyl-2-pyrrolidone (NMP), making it an eco-friendly alternative. The support membranes, prepared via UV-curing followed by non-solvent-induced phase inversion, can serve as a universal solvent-resistant support for the synthesis of a broad set of membranes, for which the selective layer can be deposited from any solvent. Parameters such as UV irradiation time and intensity, as well as the concentrations of PSf, penta-acrylate, and photo-initiator in the casting solution were varied to obtain such supports. The characteristics of the resulting supports were investigated in terms of separation performance, hydrophobicity, porosity, degree of acrylate conversion, and pure water flux. The resulting membranes showed improved chemical resistance in solvents such as ethyl acetate, NMP, tetrahydrofuran (THF), and toluene. Solvent-resistant supports with different pore sizes were synthesized and used for the preparation of thin film composite (TFC) membranes to demonstrate their potential. Promising separation performances with Rose Bengal (RB) rejections up to 98% and water permeances up to 1.5 L m−2 h−1 bar−1 were reached with these TFC-membranes carrying a polyamide top layer synthesized via interfacial polymerization.
Membranes for the Capture and Screening of Waterborne Plutonium Based on a Novel Pu-Extractive Copolymer Additive
Authors: Foster James C. , DeVol Timothy A. , Husson Scott M.
Published: December 2021

This contribution describes the fabrication of plutonium-adsorptive membranes by non-solvent induced phase separation. The dope solution comprised poly(vinylidene fluoride) (PVDF) and a Pu-extractive copolymer additive of PVDF-g-poly(ethylene glycol methacrylate phosphate) (EGMP) in dimethylformamide (DMF). The effects of casting conditions on membrane permeability were determined for PVDF membranes prepared with 10 wt% PVDF-g-EGMP. Direct-flow filtration and alpha spectrometry showed that membranes containing the graft copolymer could recover Pu up to 59.9 ± 3.0% from deionized water and 19.3 ± 3.5% from synthetic seawater after filtering 10 mL of 0.5 Bq/mL 238Pu. SEM-EDS analysis indicated that the graft copolymer was distributed evenly throughout the entire depth of the copolymer membranes, likely attributing to the tailing observed in the alpha spectra for 238Pu. Despite the reduction in resolution, the membranes exhibited high Pu uptake at the conditions tested, and new membrane designs that promote copolymer surface migration are expected to improve alpha spectrometry peak energy resolutions. Findings from this study also can be used to guide the development of extractive membranes for chromatographic separation of actinides from contaminated groundwater sources.
Enhancing Absorption Performance of CO2 by Amine Solution through the Spiral Wired Channel in Concentric Circular Membrane Contactors
Authors: Ho Chii-Dong , Chang Hsuan , Lin Guan-Hong , Chew Thiam Leng
Published: December 2021

The CO2 absorption rate by using a Monoethanolamide (MEA) solution through the spiral wired channel in concentric circular membrane contactors under both concurrent-flow and countercurrent-flow operations was investigated experimentally and theoretically. The one-dimensional mathematical modeling equation developed for predicting the absorption rate and concentration distributions was solved numerically using the fourth Runge–Kutta method under various absorbent flow rate, CO2 feed flow rate and inlet CO2 concentration in the gas feed. An economical viewpoint of the spiral wired module was examined by assessing both absorption flux improvement and power consumption increment. Meanwhile, the correlated average Sherwood number to predict the mass-transfer coefficient of the CO2 absorption mechanisms in a concentric circular membrane contactor with the spiral wired annulus channel is also obtained in a generalized and simplified expression. The theoretical predictions of absorption flux improvement were validated by experimental results in good agreements. The amine solution flowing through the annulus of a concentric circular tube, which was inserted in a tight-fitting spiral wire in a small annular spacing, could enhance the CO2 absorption flux improvement due to reduction of the concentration polarization effect. A larger concentration polarization coefficient (CPC) was achieved in the countercurrent-flow operations than that in concurrent-flow operations for various operations conditions and spiral-wire pitches. The absorption flux improvement for inserting spiral wire in the concentric circular module could provide the maximum relative increment up to 46.45%.
Lipid Membrane State Change by Catalytic Protonation and the Implications for Synaptic Transmission
Authors: Fillafer Christian , Koll Yana S. , Schneider Matthias F.
Published: December 2021

In cholinergic synapses, the neurotransmitter acetylcholine (ACh) is rapidly hydrolyzed by esterases to choline and acetic acid (AH). It is believed that this reaction serves the purpose of deactivating ACh once it has exerted its effect on a receptor protein (AChR). The protons liberated in this reaction, however, may by themselves excite the postsynaptic membrane. Herein, we investigated the response of cell membrane models made from phosphatidylcholine (PC), phosphatidylserine (PS) and phosphatidic acid (PA) to ACh in the presence and absence of acetylcholinesterase (AChE). Without a catalyst, there were no significant effects of ACh on the membrane state (lateral pressure change ≤0.5 mN/m). In contrast, strong responses were observed in membranes made from PS and PA when ACh was applied in presence of AChE (>5 mN/m). Control experiments demonstrated that this effect was due to the protonation of lipid headgroups, which is maximal at the pK (for PS: pKCOOH≈5.0; for PA: pKHPO4−≈8.5). These findings are physiologically relevant, because both of these lipids are present in postsynaptic membranes. Furthermore, we discussed evidence which suggests that AChR assembles a lipid-protein interface that is proton-sensitive in the vicinity of pH 7.5. Such a membrane could be excited by hydrolysis of micromolar amounts of ACh. Based on these results, we proposed that cholinergic transmission is due to postsynaptic membrane protonation. Our model will be falsified if cholinergic membranes do not respond to acidification.
Investigation of the Technological Possibility of Laser Hardening of Stainless Steel 14Cr17Ni2 to a Deep Depth of the Surface
Authors: Somonov Vladislav , Tsibulskiy Igor , Mendagaliyev Ruslan , Akhmetov Alexander
Published: December 2021

The article presents the results of a research of the process of laser hardening of steel 14Cr17Ni2 (AISI 431) by radiation of a high-power fiber laser LS-16. Assessment of the theoretically possible maximum depth in laser processing without additional beam transformations, the use of additional coatings and devices were shown. The results of experiments on increasing the depth of the hardened layer during laser processing by using scanning of the laser beam and optimally selected mode parameters without scanning are demonstrated. The influence of the number of passes on the depth of the hardened layer is investigated. The microstructure of hardened samples was studied and quantitative estimation of structural components was carried out. The microhardness of hardened samples at different modes of laser hardening was measured.
Microstructure and Mechanical Properties of Ultrafine Quaternary Al-Cu-Si-Mg Eutectic Alloy
Authors: Cai Qing , Cantor Brian , Tong Vivian S. , Wang Feng , Mendis Chamini L. , Chang Isaac T.H. , Fan Zhongyun
Published: December 2021

The microstructure evolution and mechanical properties of quaternary Al-Cu-Si-Mg eutectic alloy prepared via arc melting and suction casting were studied. This alloy exhibits a single endothermic DSC peak with a melting temperature of 509 °C upon heating, suggesting a eutectic reaction. The cast alloy microstructure consisted of four phases, α-Al, Al2Cu (θ), Si and Al4Cu2Mg8Si7 (Q), in the eutectic cells and also in the nano-scale anomalous eutectic in the intercellular regions. The eutectic cells show different morphologies in different parts of the sample. Well-defined orientation relationships between the α-Al, Al2Cu, and Q phases were found in the eutectic cell centres, while decoupled growth of Q phase occurred at the cell boundaries. The bimodal microstructure exhibits excellent compressive mechanical properties, including a yield strength of 835 ± 35 MPa, a fracture strength of ~1 GPa and a compressive fracture strain of 4.7 ± 1.1%. The high strength is attributed to a combination of a refined eutectic structure and strengthening from multiple hard phases.
Opening the Random Forest Black Box of the Metabolome by the Application of Surrogate Minimal Depth
Authors: Wenck Soeren , Creydt Marina , Hansen Jule , Gärber Florian , Fischer Markus , Seifert Stephan
Published: December 2021

For the untargeted analysis of the metabolome of biological samples with liquid chromatography–mass spectrometry (LC-MS), high-dimensional data sets containing many different metabolites are obtained. Since the utilization of these complex data is challenging, different machine learning approaches have been developed. Those methods are usually applied as black box classification tools, and detailed information about class differences that result from the complex interplay of the metabolites are not obtained. Here, we demonstrate that this information is accessible by the application of random forest (RF) approaches and especially by surrogate minimal depth (SMD) that is applied to metabolomics data for the first time. We show this by the selection of important features and the evaluation of their mutual impact on the multi-level classification of white asparagus regarding provenance and biological identity. SMD enables the identification of multiple features from the same metabolites and reveals meaningful biological relations, proving its high potential for the comprehensive utilization of high-dimensional metabolomics data.
Immune Responses to SARS-CoV-2 Infection and Vaccination in Dialysis Patients and Kidney Transplant Recipients
Authors: Affeldt Patrick , Koehler Felix Carlo , Brensing Karl August , Adam Vivien , Burian Julia , Butt Linus , Gies Martin , Grundmann Franziska , Hinrichs Steffen , Johannis Wibke , Kalisch Nils , Meyer-Delpho Matthias , Oehm Simon , Platen Eva , Schöler Claudia , Heger Eva , Steger Gertrud , Stippel Dirk , Ziegelhöfer Aileen , Benzing Thomas , Klein Florian , Kurschat Christine , Müller Roman-Ulrich , Cristanziano Veronica Di
Published: December 2021

Dialysis patients and kidney transplant (KTX) recipients suffer from an impaired immune system and show a decreased response to the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) vaccination. We performed a retrospective analysis of 1505 serological SARS-CoV-2 measurements obtained from 887 dialysis patients and 86 KTX recipients. The results were separated by patient subgroups (dialysis/KTX) as well as SARS-CoV-2 status. The latter criterion included SARS-CoV-2-naïve patients with or without COVID-19 vaccination and convalescent patients receiving a booster shot. Serologies of 27 vaccinated healthy individuals served as the reference group. Vaccine-induced cellular immune response was quantified by an interferon-γ release assay in 32 KTX recipients. We determined seroconversion rates of 92.6%, 93.4%, and 71.4% in dialysis patients vaccinated with either BNT162b2, mRNA-1273, or AZD1222, respectively. Vaccination-induced anti-SARS-CoV-2 antibody titers were lower in dialysis patients compared to healthy individuals, and vaccination with mRNA-1273 induced higher titers than BNT162b2. The initial seroconversion rate was 39.5% in KTX recipients vaccinated with BNT162b2. A linear regression model identified medication with mycophenolate-mofetil/mycophenolic acid as an independent risk factor for missing seroconversion. Within a cohort of 32 KTX recipients, cellular and humoral immune reactivity to SARS-CoV-2 was detectable in three patients only. Conclusively, vaccine-induced seroconversion rates were similar in dialysis patients compared to healthy individuals but were strongly impaired in KTX recipients. Anti-SARS-CoV-2 IgG titers elicited by double active immunization were significantly lower in both cohorts compared to healthy individuals, and immune responses to vaccination vanished quickly.
Characterization of Macro Mechanical Properties and Microstructures of Cement-Based Composites Prepared from Fly Ash, Gypsum and Steel Slag
Authors: Li Jiajian , Cao Shuai , Yilmaz Erol
Published: December 2021

Using solid wastes (SWs) as backfilling material to fill underground mined-out areas (UMOAs) solved the environmental problems caused by SWs and reduced the backfilling cost. In this study, fly ash (FA), gypsum and steel slag (SS) were used to prepare cement-based composites (CBC). The uniaxial compression, computed tomography (CT) and scanning electron microscope (SEM) laboratory experiments were conducted to explore the macro and micromechanical properties of CBC. The findings showed that the uniaxial compressive strength (UCS) of CBC with a curing time of 7 d could reach 6.54 MPa. The increase of SS content reduced the UCS of CBC, while the gypsum and FA content could increase the UCS of CBC. Microscopic studies have shown that the SS particles in CBC have noticeable sedimentation, and the increase of SS content causes the failure mode of CBC from tensile to tensile-shear. These research results can provide a scientific reference for the preparation of backfilling materials.
Practical Use of Measuring the Deflection of Roof Layers in the Assessment of the Stability of Mining Excavations in the Polish Copper Ore Mine “Polkowice-Sieroszowice”
Authors: Stolecki Lech , Szczerbiński Krzysztof
Published: December 2021

The paper presents the results of long-term continuous measurements of the deflection of the roof layers in the underground copper mine Polkowice-Sieroszowice, Poland belonging to KGHM Polska Miedź S.A. The measurements were performed with the use of the inclinometric method consisting of continuous registration of changes in the angle of inclination of the roof strata. The measurements were carried out using an inclinometer sensor fixed to the end of a rockbolt in the roof. Measurements presented in the article were made in various regions of the underground mine. The monitoring covered: The exploitation front, machinery chamber and the region of the experimental longwall mining of copper ore. The obtained results proved the usefulness of the developed method in the process of the evaluation of the stability of mining excavations. The sensors were highly sensitive and performed the measurements in a simple way; highly accurate and reliable results were obtained.
Give or Take: Effects of Electron-Accepting/-Withdrawing Groups in Red-Fluorescent BODIPY Molecular Rotors
Authors: Maleckaitė Karolina , Narkevičius Domantas , Žilėnaitė Rugilė , Dodonova-Vaitkūnienė Jelena , Toliautas Stepas , Tumkevičius Sigitas , Vyšniauskas Aurimas
Published: December 2021

Mapping microviscosity, temperature, and polarity in biosystems is an important capability that can aid in disease detection. This can be achieved using fluorescent sensors based on a green-emitting BODIPY group. However, red fluorescent sensors are desired for convenient imaging of biological samples. It is known that phenyl substituents in the β position of the BODIPY core can shift the fluorescence spectra to longer wavelengths. In this research, we report how electron-withdrawing (EWG) and -donating (EDG) groups can change the spectral and sensory properties of β-phenyl-substituted BODIPYs. We present a trifluoromethyl-substituted (EWG) conjugate with moderate temperature sensing properties and a methoxy-substituted (EDG) molecule that could be used as a lifetime-based polarity probe. In this study, we utilise experimental results of steady-state and time-resolved fluorescence, as well as quantum chemical calculations using density functional theory (DFT). We also explain how the energy barrier height (Ea) for non-radiative relaxation affects the probe’s sensitivity to temperature and viscosity and provide appropriate Ea ranges for the best possible sensitivity to viscosity and temperature.
Data-Centric Architecture for Self-Driving Laboratories with Autonomous Discovery of New Nanomaterials
Authors: Butakova Maria A. , Chernov Andrey V. , Kartashov Oleg O. , Soldatov Alexander V.
Published: December 2021

Artificial intelligence (AI) approaches continue to spread in almost every research and technology branch. However, a simple adaptation of AI methods and algorithms successfully exploited in one area to another field may face unexpected problems. Accelerating the discovery of new functional materials in chemical self-driving laboratories has an essential dependence on previous experimenters’ experience. Self-driving laboratories help automate and intellectualize processes involved in discovering nanomaterials with required parameters that are difficult to transfer to AI-driven systems straightforwardly. It is not easy to find a suitable design method for self-driving laboratory implementation. In this case, the most appropriate way to implement is by creating and customizing a specific adaptive digital-centric automated laboratory with a data fusion approach that can reproduce a real experimenter’s behavior. This paper analyzes the workflow of autonomous experimentation in the self-driving laboratory and distinguishes the core structure of such a laboratory, including sensing technologies. We propose a novel data-centric research strategy and multilevel data flow architecture for self-driving laboratories with the autonomous discovery of new functional nanomaterials.
Scintillation Response Enhancement in Nanocrystalline Lead Halide Perovskite Thin Films on Scintillating Wafers
Authors: Děcká Kateřina , Král Jan , Hájek František , Průša Petr , Babin Vladimir , Mihóková Eva , Čuba Václav
Published: December 2021

Lead halide perovskite nanocrystals of the formula CsPbBr3 have recently been identified as potential time taggers in scintillating heterostructures for time-of-flight positron emission tomography (TOF-PET) imaging thanks to their ultrafast decay kinetics. This study investigates the potential of this material experimentally. We fabricated CsPbBr3 thin films on scintillating GGAG:Ce (Gd2.985Ce0.015Ga2.7Al2.3O12) wafer as a model structure for the future sampling detector geometry. We focused this study on the radioluminescence (RL) response of this composite material. We compare the results of two spin-coating methods, namely the static and the dynamic process, for the thin film preparation. We demonstrated enhanced RL intensity of both CsPbBr3 and GGAG:Ce scintillating constituents of a composite material. This synergic effect arises in both the RL spectra and decays, including decays in the short time window (50 ns). Consequently, this study confirms the applicability of CsPbBr3 nanocrystals as efficient time taggers for ultrafast timing applications, such as TOF-PET.
Effective Double Electron Transport Layer Inducing Crystallization of Active Layer for Improving the Performance of Organic Solar Cells
Authors: Li Ping , Chen Lijia , Hu Xiaoyan , He Lirong , Jiang Zezhuan , Luo Minghao , Huang Haishen , Yuan Wei , He Yinghu
Published: December 2021

Interface modification plays an important role in enhancing the photoelectric conversion efficiency and stability of organic solar cells. In this work, alkali metal lithium chloride (LiCl) was introduced between indium tin oxide and polyethyleneimine ethoxylate (PEIE) to prepare a double-layer electron transport layer. Results show that the introduction of LiCl has dual functions. The first function is that LiCl can enhance conductivity, thereby facilitating charge collection. The second function is that the double-layer electron transport layer based on LiCl can induce the crystallization of active layer, thereby enhancing charge transport. Devices with LiCl/PEIE double layer achieve a high power conversion efficiency (PCE) of 3.84%, which is 21.5% higher than that of pristine devices (the PCE of pristine devices with pure PEIE interface layer is 3.16%).
Comment on Klek et al. Enhanced Recovery after Surgery (ERAS) Protocol Is a Safe and Effective Approach in Patients with Gastrointestinal Fistulas Undergoing Reconstruction: Results from a Prospective Study. Nutrients 2021, 13, 1953
Authors: Lauro Augusto , Ripoli Maria Cristina
Published: December 2021

We read and appreciated the prospective study by Klek et al. [...]
Kinetics of Blood–Brain Barrier Transport of Monoclonal Antibodies Targeting the Insulin Receptor and the Transferrin Receptor
Authors: Pardridge William M.
Published: December 2021

Biologic drugs are large molecule pharmaceuticals that do not cross the blood–brain barrier (BBB), which is formed by the brain capillary endothelium. Biologics can be re-engineered for BBB transport as IgG fusion proteins, where the IgG domain is a monoclonal antibody (MAb) that targets an endogenous BBB transporter, such as the insulin receptor (IR) or transferrin receptor (TfR). The IR and TfR at the BBB transport the receptor-specific MAb in parallel with the transport of the endogenous ligand, insulin or transferrin. The kinetics of BBB transport of insulin or transferrin, or an IRMAb or TfRMAb, can be quantified with separate mathematical models. Mathematical models to estimate the half-time of receptor endocytosis, MAb or ligand exocytosis into brain extracellular space, or receptor recycling back to the endothelial luminal membrane were fit to the brain uptake of a TfRMAb or a IRMAb fusion protein in the Rhesus monkey. Model fits to the data also allow for estimates of the rates of association of the MAb in plasma with the IR or TfR that is embedded within the endothelial luminal membrane in vivo. The parameters generated from the model fits can be used to estimate the brain concentration profile of the MAb over time, and this brain exposure is shown to be a function of the rate of clearance of the antibody fusion protein from the plasma compartment.
An Insight into the Abiotic Stress Responses of Cultivated Beets (Beta vulgaris L.)
Authors: Yolcu Seher , Alavilli Hemasundar , Ganesh Pushpalatha , Asif Muhammad , Kumar Manu , Song Kihwan
Published: December 2021

Cultivated beets (sugar beets, fodder beets, leaf beets, and garden beets) belonging to the species Beta vulgaris L. are important sources for many products such as sugar, bioethanol, animal feed, human nutrition, pulp residue, pectin extract, and molasses. Beta maritima L. (sea beet or wild beet) is a halophytic wild ancestor of all cultivated beets. With a requirement of less water and having shorter growth period than sugarcane, cultivated beets are preferentially spreading from temperate regions to subtropical countries. The beet cultivars display tolerance to several abiotic stresses such as salt, drought, cold, heat, and heavy metals. However, many environmental factors adversely influence growth, yield, and quality of beets. Hence, selection of stress-tolerant beet varieties and knowledge on the response mechanisms of beet cultivars to different abiotic stress factors are most required. The present review discusses morpho-physiological, biochemical, and molecular responses of cultivated beets (B. vulgaris L.) to different abiotic stresses including alkaline, cold, heat, heavy metals, and UV radiation. Additionally, we describe the beet genes reported for their involvement in response to these stress conditions.
Crosstalk between Calcium and ROS Signaling during Flg22-Triggered Immune Response in Arabidopsis Leaves
Authors: Marcec Matthew J. , Tanaka Kiwamu
Published: December 2021

Calcium and reactive oxygen species (ROS) are two of the earliest second messengers in response to environmental stresses in plants. The rise and sequestration of these messengers in the cytosol and apoplast are formed by various channels, transporters, and enzymes that are required for proper defense responses. It remains unclear how calcium and ROS signals regulate each other during pattern-triggered immunity (PTI). In the present study, we examined the effects of perturbing one signal on the other in Arabidopsis leaves upon the addition of flg22, a well-studied microbe-associated molecular pattern (MAMP). To this end, a variety of pharmacological agents were used to suppress either calcium or ROS signaling. Our data suggest that cytosolic calcium elevation is required to initiate and regulate apoplastic ROS production generated by respiratory burst oxidase homologs (RBOHs). In contrast, ROS has no effect on the initiation of the calcium signal, but is required for forming a sufficient amplitude of the calcium signal. This finding using pharmacological agents is corroborated by the result of using a genetic double mutant, rbohd rbohf. Our study provides an insight into the mutual interplay of calcium and ROS signals during the MAMP-induced PTI response in plants.