bluuebunny/crossref_metadata_2025 huggingface dataset:
Offering research education for in-service language teachers
Authors: Bardel Camilla , Erickson Gudrun , Granfeldt Jonas , Rosén Christina
Published: March 2017

Since 2008, the Swedish government has launched occasional offers of funding for graduate schools aimed at practising teachers. The fundamental purpose of this initiative is to enhance quality in the Swedish school system by implementing what is stated in the Education Act, namely that education at all levels should be based upon scientific knowledge and evidence-based experience.
Second language writing development: A research agenda
Authors: Polio Charlene
Published: March 2017

In 1998, Wolfe-Quintero, Inagaki & Kim published a monograph describing measures used in assessing writing development. Despite more recent research on linguistic development (e.g., Bulté & Housen 2012; Verspoor, Schmid & Xu 2012; Connor-Linton & Polio 2014), the volume is still a valuable resource and good starting point for anyone wanting to select measures of fluency, accuracy, and complexity for second language (L2) writing research. The volume, however, was limited to research onlanguagedevelopment within the context of writing, and this is certainly one way to think about writing development. A recent edited volume by Manchón (2012), however, has greatly expanded conceptions of writing development showing that it involves much more that linguistic development, for example, genre knowledge (Tardy 2012) and goal setting (Cumming 2012) as well. Writing development can also focus on various aspects of the writing process and how writers’ change their approach to text production as they become more expert writers (e.g., Sasaki 2004; Nicolás-Conesa, Roca de Larios & Coyle 2014). This expansion of focus, of course, makes development more difficult to define.
Language education policy and practice in East and Southeast Asia
Authors: Kirkpatrick Andy , Liddicoat Anthony J.
Published: March 2017

East and Southeast Asia represents a linguistically and culturally diverse region. For example, more than 700 languages are spoken in Indonesia alone. It is against this backdrop of diversity that the ten countries that comprise Association of South East Asian Nations (ASEAN) have recently signed the ASEAN Charter which, while calling for respect for the region's languages, cultures and religions also officially nominates English as ASEAN's working language. In this article, we examine the language education policies of the region and consider the implications of these policies for the maintenance of linguistic and cultural diversity on the one hand and the promotion of English and the respective national languages on the other. As ASEAN is closely connected to the three major countries of China, Japan and South Korea, as indicated by the ‘ASEAN + 3’ forum, we also include these countries here. We stress that, as space forbids an in-depth treatment of the language education policies of each of the 13 countries, we have chosen to describe and discuss in some depth the policies of 5 countries (China, Indonesia, Japan, the Philippines and Vietnam), as these provide a cross-section of language policy contexts and approaches in the region. We add brief notes on the policies of the remaining countries.
An efficient W-band InP DHBT digital power amplifier
Authors: Wentzel Andreas , Hossain Maruf , Stoppel Dimitri , Weimann Nils , Krozer Viktor , Heinrich Wolfgang
Published: March 2017

This paper presents for the first time high-efficiency W-band power amplifiers (PAs), the design of which follows the digital PA (DPA) design concept. Two DPAs with different output networks have been realized: a single-band version (S-DPA) for 95 GHz and a dual-band design (D-DPA) for signal frequencies fSof 68 GHz (first band) and 76 GHz (second band), respectively. The PAs are realized as monolithic microwave-integrated circuits (MMICs) in a 0.8 μm InP DHBT transferred-substrate process. They utilize a double-emitter-finger DHBT unit cell with an emitter area of 2 × 0.8 × 6 μm3each. In contrast to the usual W-band PAs, the proposed single-stage amplifier MMICs do not apply any special reactive matching for the transistor, which leads to very compact chip sizes of 0.27 mm2(S-DPA) and 0.39 mm2(D-DPA). The S-DPA includes one band-pass filter (BPF) at the output with 0.6 dB insertion loss (IL) and 24 dB input return loss (RL) at the signal frequency of 95 GHz. The dual-band BPF shows 0.7 dB IL in both bands with a RL of more than 21 dB each. Applying an overdriven sinusoidal input signal to emulate digital operation the DPAs achieve a maximum output power of 14.4 dBm and power-added efficiency of 31% when using the single-band configuration. Collector efficiencies of more than 80% and the flexible multi-band operation demonstrated prove the great potential of the digital PA concept for future high-speed communications.
Tracking Volcanic Bombs in Three Dimensions
Authors: Crane Leah
Published: March 2017

A new method allows researchers to precisely track in three dimensions bits of fragmented magma as they are expelled in explosive volcanic eruptions.
Global histone modification fingerprinting in human cells using epigenetic reverse phase protein array
Authors: Partolina Marina , Thoms Hazel C , MacLeod Kenneth G , Rodriguez-Blanco Giovanny , Clarke Matthew N , Venkatasubramani Anuroop V , Beesoo Rima , Larionov Vladimir , Neergheen-Bhujun Vidushi S , Serrels Bryan , Kimura Hiroshi , Carragher Neil O , Kagansky Alexander
Published: March 2017

The balance between acetylation and deacetylation of histone proteins plays a critical role in the regulation of genomic functions. Aberrations in global levels of histone modifications are linked to carcinogenesis and are currently the focus of intense scrutiny and translational research investments to develop new therapies, which can modify complex disease pathophysiology through epigenetic control. However, despite significant progress in our understanding of the molecular mechanisms of epigenetic machinery in various genomic contexts and cell types, the links between epigenetic modifications and cellular phenotypes are far from being clear. For example, enzymes controlling histone modifications utilize key cellular metabolites associated with intra- and extracellular feedback loops, adding a further layer of complexity to this process. Meanwhile, it has become increasingly evident that new assay technologies which provide robust and precise measurement of global histone modifications are required, for at least two pressing reasons: firstly, many approved drugs are known to influence histone modifications and new cancer therapies are increasingly being developed towards targeting histone deacetylases (HDACs) and other epigenetic readers and writers. Therefore, robust assays for fingerprinting the global effects of such drugs on preclinical cell, organoid and in vivo models is required; and secondly, robust histone-fingerprinting assays applicable to patient samples may afford the development of next-generation diagnostic and prognostic tools. In our study, we have used a panel of monoclonal antibodies to determine the relative changes in the global abundance of post-translational modifications on histones purified from cancer cell lines treated with HDAC inhibitors using a novel technique, called epigenetic reverse phase protein array. We observed a robust increase in acetylation levels within 2–24 h after inhibition of HDACs in different cancer cell lines. Moreover, when these cells were treated with N-acetylated amino acids in addition to HDACs, we detected a further increase in histone acetylation, demonstrating that these molecules could be utilized as donors of the acetyl moiety for protein acetylation. Consequently, this study not only offers a novel assay for diagnostics and drug screening but also warrants further research of the novel class of inexpensive, non-toxic natural compounds that could potentiate the effects of HDAC inhibitors and is therefore of interest for cancer therapeutics.
Lysogeny in nature: mechanisms, impact and ecology of temperate phages
Authors: Howard-Varona Cristina , Hargreaves Katherine R , Abedon Stephen T , Sullivan Matthew B
Published: March 2017

Viruses that infect bacteria (phages) can influence bacterial community dynamics, bacterial genome evolution and ecosystem biogeochemistry. These influences differ depending on whether phages establish lytic, chronic or lysogenic infections. Although the first two produce virion progeny, with lytic infections resulting in cell destruction, phages undergoing lysogenic infections replicate with cells without producing virions. The impacts of lysogeny are numerous and well-studied at the cellular level, but ecosystem-level consequences remain underexplored compared to those of lytic infections. Here, we review lysogeny from molecular mechanisms to ecological patterns to emerging approaches of investigation. Our goal is to highlight both its diversity and importance in complex communities. Altogether, using a combined viral ecology toolkit that is applied across broad model systems and environments will help us understand more of the diverse lifestyles and ecological impacts of lysogens in nature.
A general derivation and quantification of the third law of thermodynamics
Authors: Masanes Lluís , Oppenheim Jonathan
Published: March 2017

The most accepted version of the third law of thermodynamics, the unattainability principle, states that any process cannot reach absolute zero temperature in a finite number of steps and within a finite time. Here, we provide a derivation of the principle that applies to arbitrary cooling processes, even those exploiting the laws of quantum mechanics or involving an infinite-dimensional reservoir. We quantify the resources needed to cool a system to any temperature, and translate these resources into the minimal time or number of steps, by considering the notion of a thermal machine that obeys similar restrictions to universal computers. We generally find that the obtainable temperature can scale as an inverse power of the cooling time. Our results also clarify the connection between two versions of the third law (the unattainability principle and the heat theorem), and place ultimate bounds on the speed at which information can be erased.
Suppressive IL-17A+Foxp3+ and ex-Th17 IL-17AnegFoxp3+ Treg cells are a source of tumour-associated Treg cells
Authors: Downs-Canner Stephanie , Berkey Sara , Delgoffe Greg M. , Edwards Robert P. , Curiel Tyler , Odunsi Kunle , Bartlett David L. , Obermajer Nataša
Published: March 2017

Th17 and regulatory T (Treg) cells are integral in maintaining immune homeostasis and Th17–Treg imbalance is associated with inflammatory immunosuppression in cancer. Here we show that Th17 cells are a source of tumour-induced Foxp3+ cells. In addition to natural (n)Treg and induced (i)Treg cells that develop from naive precursors, suppressive IL-17A+Foxp3+ and ex-Th17 Foxp3+ cells are converted from IL-17A+Foxp3neg cells in tumour-bearing mice. Metabolic phenotyping of Foxp3-expressing IL-17A+, ex-Th17 and iTreg cells demonstrates the dissociation between the metabolic fitness and the suppressive function of Foxp3-expressing Treg cell subsets. Although all Foxp3-expressing subsets are immunosuppressive, glycolysis is a prominent metabolic pathway exerted only by IL-17A+Foxp3+ cells. Transcriptome analysis and flow cytometry of IL-17A+Foxp3+ cells indicate that Folr4, GARP, Itgb8, Pglyrp1, Il1rl1, Itgae, TIGIT and ICOS are Th17-to-Treg cell transdifferentiation-associated markers. Tumour-associated Th17-to-Treg cell conversion identified here provides insights for targeting the dynamism of Th17–Treg cells in cancer immunotherapy.
An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells
Authors: Trakarnsanga Kongtana , Griffiths Rebecca E. , Wilson Marieangela C. , Blair Allison , Satchwell Timothy J. , Meinders Marjolein , Cogan Nicola , Kupzig Sabine , Kurita Ryo , Nakamura Yukio , Toye Ashley M. , Anstee David J. , Frayne Jan
Published: March 2017

With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.
Experimental detection of entanglement polytopes via local filters
Authors: Zhao Yuan-Yuan , Grassl Markus , Zeng Bei , Xiang Guo-Yong , Zhang Chao , Li Chuan-Feng , Guo Guang-Can
Published: March 2017

Quantum entanglement, resulting in correlations between subsystems that are stronger than any possible classical correlation, is one of the mysteries of quantum mechanics. Entanglement cannot be increased by any local operation, and for a sufficiently large many-body quantum system there exist infinitely many different entanglement classes, i.e., states that are not related by stochastic local operations and classical communications. On the other hand, the method of entanglement polytopes results in finitely many coarse-grained types of entanglement that can be detected by only measuring single-particle spectra. We find, however, that with high probability the local spectra lie in more than one polytope, hence providing only partial information about the entanglement type. To overcome this problem, we propose to additionally use so-called local filters, which are non-unitary local operations. We experimentally demonstrate the detection of entanglement polytopes in a four-qubit system. Using local filters we can distinguish the entanglement type of states with the same single particle spectra, but which belong to different polytopes.
Daemonic ergotropy: enhanced work extraction from quantum correlations
Authors: Francica Gianluca , Goold John , Plastina Francesco , Paternostro Mauro
Published: March 2017

We investigate how the presence of quantum correlations can influence work extraction in closed quantum systems, establishing a new link between the field of quantum non-equilibrium thermodynamics and the one of quantum information theory. We consider a bipartite quantum system and we show that it is possible to optimize the process of work extraction, thanks to the correlations between the two parts of the system, by using an appropriate feedback protocol based on the concept of ergotropy. We prove that the maximum gain in the extracted work is related to the existence of quantum correlations between the two parts, quantified by either quantum discord or, for pure states, entanglement. We then illustrate our general findings on a simple physical situation consisting of a qubit system.
Erratum: g-force induced giant efficiency of nanoparticles internalization into living cells
Authors: Ocampo Sandra M. , Rodriguez Vanessa , de la Cueva Leonor , Salas Gorka , Carrascosa Jose L. , Rodríguez María Josefa , García-Romero Noemí , Cuñado Jose Luis F. , Camarero Julio , Miranda Rodolfo , Belda-Iniesta Cristobal , Ayuso-Sacido Angel
Published: March 2017

Nanotechnology plays an increasingly important role in the biomedical arena. Iron oxide nanoparticles (IONPs)-labelled cells is one of the most promising approaches for a fast and reliable evaluation of grafted cells in both preclinical studies and clinical trials. Current procedures to label living cells with IONPs are based on direct incubation or physical approaches based on magnetic or electrical fields, which always display very low cellular uptake efficiencies. Here we show that centrifugation-mediated internalization (CMI) promotes a high uptake of IONPs in glioblastoma tumour cells, just in a few minutes, and via clathrin-independent endocytosis pathway. CMI results in controllable cellular uptake efficiencies at least three orders of magnitude larger than current procedures. Similar trends are found in human mesenchymal stem cells, thereby demonstrating the general feasibility of the methodology, which is easily transferable to any laboratory with great potential for the development of improved biomedical applications.
Correction: Corrigendum: Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion
Authors: Yata Tomoya , Takahashi Yuki , Tan Mengmeng , Hidaka Kumi , Sugiyama Hiroshi , Endo Masayuki , Takakura Yoshinobu , Nishikawa Makiya
Published: March 2017

The application of DNA as a functional material such as DNA hydrogel has attracted much attention. Despite an increasing interest, the high cost of DNA synthesis is a limiting factor for its utilization. To reduce the cost, we report here a highly efficient amplification technique for polypod-like structured DNA (polypodna) with adhesive ends that spontaneously forms DNA hydrogel. Two types of polypodna with three (tripodna) and four (tetrapodna) pods were selected, and a template oligodeoxynucleotide, containing a tandem sequence of a looped tripodna or tetrapodna, respectively, along with restriction enzyme (TspRI) sites, was designed. The template was circularized using T4 DNA ligase, and amplified by rolling circle amplification (RCA). The RCA product was highly viscous and resistant to restriction digestion. Observation under an electron microscope revealed microflower-like structures. These structures were composed of long DNA and magnesium pyrophosphate, and their treatment with EDTA followed by restriction digestion with TspRI resulted in numerous copies of polypodna with adhesive ends, which formed a DNA hydrogel. Thus, we believe this technique provides a new approach to produce DNA nanostructures, and helps in expanding their practical applications.
Compact silicon multimode waveguide spectrometer with enhanced bandwidth
Authors: Piels Molly , Zibar Darko
Published: March 2017

Compact, broadband, and high-resolution spectrometers are appealing for sensing applications, but difficult to fabricate. Here we show using calibration data a spectrometer based on a multimode waveguide with 2 GHz resolution, 250 GHz bandwidth, and a 1.6 mm × 2.1 mm footprint. Typically, such spectrometers have a bandwidth limited by the number of modes supported by the waveguide. In this case, an on-chip mode-exciting element is used to repeatably excite distinct collections of waveguide modes. This increases the number of independent spectral channels from the number of modes to this number squared, resulting in an extension of the usable range.
Disease dynamics in a stochastic network game: a little empathy goes a long way in averting outbreaks
Authors: Eksin Ceyhun , Shamma Jeff S. , Weitz Joshua S.
Published: March 2017

Individuals change their behavior during an epidemic in response to whether they and/or those they interact with are healthy or sick. Healthy individuals may utilize protective measures to avoid contracting a disease. Sick individuals may utilize preemptive measures to avoid spreading a disease. Yet, in practice both protective and preemptive changes in behavior come with costs. This paper proposes a stochastic network disease game model that captures the self-interests of individuals during the spread of a susceptible-infected-susceptible disease. In this model, individuals strategically modify their behavior based on current disease conditions. These reactions influence disease spread. We show that there is a critical level of concern, i.e., empathy, by the sick individuals above which disease is eradicated rapidly. Furthermore, we find that risk averse behavior by the healthy individuals cannot eradicate the disease without the preemptive measures of the sick individuals. Empathy is more effective than risk-aversion because when infectious individuals change behavior, they reduce all of their potential infections, whereas when healthy individuals change behavior, they reduce only a small portion of potential infections. This imbalance in the role played by the response of the infected versus the susceptible individuals on disease eradication affords critical policy insights.
SEDA: A software package for the Statistical Earthquake Data Analysis
Authors: Lombardi A. M.
Published: March 2017

In this paper, the first version of the software SEDA (SEDAv1.0), designed to help seismologists statistically analyze earthquake data, is presented. The package consists of a user-friendly Matlab-based interface, which allows the user to easily interact with the application, and a computational core of Fortran codes, to guarantee the maximum speed. The primary factor driving the development of SEDA is to guarantee the research reproducibility, which is a growing movement among scientists and highly recommended by the most important scientific journals. SEDAv1.0 is mainly devoted to produce accurate and fast outputs. Less care has been taken for the graphic appeal, which will be improved in the future. The main part of SEDAv1.0 is devoted to the ETAS modeling. SEDAv1.0 contains a set of consistent tools on ETAS, allowing the estimation of parameters, the testing of model on data, the simulation of catalogs, the identification of sequences and forecasts calculation. The peculiarities of routines inside SEDAv1.0 are discussed in this paper. More specific details on the software are presented in the manual accompanying the program package.
Transcriptome analysis of a wild bird reveals physiological responses to the urban environment
Authors: Watson Hannah , Videvall Elin , Andersson Martin N. , Isaksson Caroline
Published: March 2017

Identifying the molecular basis of environmentally induced phenotypic variation presents exciting opportunities for furthering our understanding of how ecological processes and the environment can shape the phenotype. Urban and rural environments present free-living organisms with different challenges and opportunities, which have marked consequences for the phenotype, yet little is known about responses at the molecular level. We characterised transcriptomes from an urban and a rural population of great titsParus major, demonstrating striking differences in gene expression profiles in both blood and liver tissues. Differentially expressed genes had functions related to immune and inflammatory responses, detoxification, protection against oxidative stress, lipid metabolism, and regulation of gene expression. Many genes linked to stress responses were expressed at higher levels in the urban birds, in accordance with our prediction that urban animals are exposed to greater environmental stress. This is one of the first studies to reveal transcriptional differences between urban- and rural-dwelling animals and suggests an important role for epigenetics in mediating environmentally induced physiological variation. The study provides valuable resources for developing further in-depth studies of the mechanisms driving phenotypic variation in the urban context at larger spatial and temporal scales.
Dramatic Changes in Thermoelectric Power of Germanium under Pressure: Printing n–p Junctions by Applied Stress
Authors: Korobeinikov Igor V. , Morozova Natalia V. , Shchennikov Vladimir V. , Ovsyannikov Sergey V.
Published: March 2017

Controlled tuning the electrical, optical, magnetic, mechanical and other characteristics of the leading semiconducting materials is one of the primary technological challenges. Here, we demonstrate that the electronic transport properties of conventional single-crystalline wafers of germanium may be dramatically tuned by application of moderate pressures. We investigated the thermoelectric power (Seebeck coefficient) of p– and n– type germanium under high pressure to 20 GPa. We established that an applied pressure of several GPa drastically shifts the electrical conduction to p– type. The p– type conduction is conserved across the semiconductor-metal phase transition at near 10 GPa. Upon pressure releasing, germanium transformed to a metastable st12 phase (Ge-III) with n– type semiconducting conductivity. We proposed that the unusual electronic properties of germanium in the original cubic-diamond-structured phase could result from a splitting of the “heavy” and “light” holes bands, and a related charge transfer between them. We suggested new innovative applications of germanium, e.g., in technologies of printing of n–p and n–p–n junctions by applied stress. Thus, our work has uncovered a new face of germanium as a ‘smart’ material.
Complementary transcriptomic and proteomic analyses reveal regulatory mechanisms of milk protein production in dairy cows consuming different forages
Authors: Dai Wenting , Chen Qiong , Wang Quanjuan , White Robin R. , Liu Jianxin , Liu Hongyun
Published: March 2017

Forage plays a critical role in the milk production of dairy cows; however, the mechanisms regulating bovine milk synthesis in dairy cows fed high forage rations with different basal forage types are not well-understood. In the study, rice straw (RS, low-quality) and alfalfa hay (AH, high-quality) diets were fed to lactating cows to explore how forage quality affected the molecular mechanisms regulating milk production using RNA-seq transcriptomic method with iTRAQ proteomic technique. A total of 554 transcripts (423 increased and 131 decreased) and 517 proteins (231 up-regulated and 286 down-regulated) were differentially expressed in the mammary glands of the two groups. The correlation analysis demonstrated seven proteins (six up-regulated and one down-regulated) had consistent mRNA expression. Functional analysis of the differentially expressed transcripts/proteins suggested that enhanced capacity for energy and fatty acid metabolism, increased protein degradation, reduced protein synthesis, decreased amino acid metabolism and depressed cell growth were related to RS consumption. The results indicated cows consuming RS diets may have had depressed milk protein synthesis because these animals had decreased capacity for protein synthesis, enhanced proteolysis, inefficient energy generation and reduced cell growth. Additional work evaluating RS- and AH-based rations may help better isolate molecular adaptations to low nutrient availability during lactation.
Systematic errors in detecting biased agonism: Analysis of current methods and development of a new model-free approach
Authors: Onaran H. Ongun , Ambrosio Caterina , Uğur Özlem , Madaras Koncz Erzsebet , Grò Maria Cristina , Vezzi Vanessa , Rajagopal Sudarshan , Costa Tommaso
Published: March 2017

Discovering biased agonists requires a method that can reliably distinguish the bias in signalling due to unbalanced activation of diverse transduction proteins from that of differential amplification inherent to the system being studied, which invariably results from the non-linear nature of biological signalling networks and their measurement. We have systematically compared the performance of seven methods of bias diagnostics, all of which are based on the analysis of concentration-response curves of ligands according to classical receptor theory. We computed bias factors for a number of β-adrenergic agonists by comparing BRET assays of receptor-transducer interactions with Gs, Gi and arrestin. Using the same ligands, we also compared responses at signalling steps originated from the same receptor-transducer interaction, among which no biased efficacy is theoretically possible. In either case, we found a high level of false positive results and a general lack of correlation among methods. Altogether this analysis shows that all tested methods, including some of the most widely used in the literature, fail to distinguish true ligand bias from “system bias” with confidence. We also propose two novel semi quantitative methods of bias diagnostics that appear to be more robust and reliable than currently available strategies.
Identification of Elongated Primary Cilia with Impaired Mechanotransduction in Idiopathic Scoliosis Patients
Authors: Oliazadeh Niaz , Gorman Kristen F. , Eveleigh Robert , Bourque Guillaume , Moreau Alain
Published: March 2017

The primary cilium is an outward projecting antenna-like organelle with an important role in bone mechanotransduction. The capacity to sense mechanical stimuli can affect important cellular and molecular aspects of bone tissue. Idiopathic scoliosis (IS) is a complex pediatric disease of unknown cause, defined by abnormal spinal curvatures. We demonstrate significant elongation of primary cilia in IS patient bone cells. In response to mechanical stimulation, these IS cells differentially express osteogenic factors, mechanosensitive genes, and signaling genes. Considering that numerous ciliary genes are associated with a scoliosis phenotype, among ciliopathies and knockout animal models, we expected IS patients to have an accumulation of rare variants in ciliary genes. Instead, our SKAT-O analysis of whole exomes showed an enrichment among IS patients for rare variants in genes with a role in cellular mechanotransduction. Our data indicates defective cilia in IS bone cells, which may be linked to heterogeneous gene variants pertaining to cellular mechanotransduction.
The evolution of extreme cooperation via shared dysphoric experiences
Authors: Whitehouse Harvey , Jong Jonathan , Buhrmester Michael D. , Gómez Ángel , Bastian Brock , Kavanagh Christopher M. , Newson Martha , Matthews Miriam , Lanman Jonathan A. , McKay Ryan , Gavrilets Sergey
Published: March 2017

Willingness to lay down one’s life for a group of non-kin, well documented historically and ethnographically, represents an evolutionary puzzle. Building on research in social psychology, we develop a mathematical model showing how conditioning cooperation on previous shared experience can allow individually costly pro-group behavior to evolve. The model generates a series of predictions that we then test empirically in a range of special sample populations (including military veterans, college fraternity/sorority members, football fans, martial arts practitioners, and twins). Our empirical results show that sharing painful experiences produces “identity fusion” – a visceral sense of oneness – which in turn can motivate self-sacrifice, including willingness to fight and die for the group. Practically, our account of how shared dysphoric experiences produce identity fusion helps us better understand such pressing social issues as suicide terrorism, holy wars, sectarian violence, gang-related violence, and other forms of intergroup conflict.
An advanced phase retrieval algorithm in N-step phase-shifting interferometry with unknown phase shifts
Authors: Li Jiaosheng , Zhong Liyun , Liu Shengde , Zhou Yunfei , Xu Jie , Tian Jindong , Lu Xiaoxu
Published: March 2017

In phase-shifting interferometry with unknown phase shifts, a normalization and orthogonalization phase-shifting algorithm (NOPSA) is proposed to achieve phase retrieval. The background of interferogram is eliminated through using the orthogonality of complex sinusoidal function; and the influence of phase shifts deviation on accuracy of phase retrieval is avoided through both normalization and orthogonalization processing. Compared with the current algorithms with unknown phase shifts, the proposed algorithm reveals significantly faster computation speed, higher accuracy, better stability and non-sensitivity of phase shifts deviation.
A retrospective cohort study to quantify the contribution of health systems to child survival in Kenya: 1996–2014
Authors: Anthopolos Rebecca , Simmons Ryan , O’Meara Wendy Prudhomme
Published: March 2017

Globally, the majority of childhood deaths in the post-neonatal period are caused by infections that can be effectively treated or prevented with inexpensive interventions delivered through even very basic health facilities. To understand the role of inadequate health systems on childhood mortality in Kenya, we assemble a large, retrospective cohort of children (born 1996–2013) and describe the health systems context of each child using health facility survey data representative of the province at the time of a child’s birth. We examine the relationship between survival beyond 59 months of age and geographic distribution of health facilities, quality of services, and cost of services. We find significant geographic heterogeneity in survival that can be partially explained by differences in distribution of health facilities and user fees. Higher per capita density of health facilities resulted in a 25% reduction in the risk of death (HRR = 0.73, 95% CI:0.58 to 0.91) and accounted for 30% of the between-province heterogeneity in survival. User fees for sick-child visits increased risk by 30% (HRR = 1.30, 95% CI:1.11 to 1.53). These results implicate health systems constraints in child mortality, quantify the contribution of specific domains of health services, and suggest priority areas for improvement to accelerate reductions in child mortality.
Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient
Authors: Shimojima Masaya , Yuasa Shinsuke , Motoda Chikaaki , Yozu Gakuto , Nagai Toshihiro , Ito Shogo , Lachmann Mark , Kashimura Shin , Takei Makoto , Kusumoto Dai , Kunitomi Akira , Hayashiji Nozomi , Seki Tomohisa , Tohyama Shugo , Hashimoto Hisayuki , Kodaira Masaki , Egashira Toru , Hayashi Kenshi , Nakanishi Chiaki , Sakata Kenji , Yamagishi Masakazu , Fukuda Keiichi
Published: March 2017

Alteration of the nuclear Ca2+ transient is an early event in cardiac remodeling. Regulation of the nuclear Ca2+ transient is partly independent of the cytosolic Ca2+ transient in cardiomyocytes. One nuclear membrane protein, emerin, is encoded by EMD, and an EMD mutation causes Emery-Dreifuss muscular dystrophy (EDMD). It remains unclear whether emerin is involved in nuclear Ca2+ homeostasis. The aim of this study is to elucidate the role of emerin in rat cardiomyocytes by means of hypertrophic stimuli and in EDMD induced pluripotent stem (iPS) cell-derived cardiomyocytes in terms of nuclear structure and the Ca2+ transient. The cardiac hypertrophic stimuli increased the nuclear area, decreased nuclear invagination, and increased the half-decay time of the nuclear Ca2+ transient in cardiomyocytes. Emd knockdown cardiomyocytes showed similar properties after hypertrophic stimuli. The EDMD-iPS cell-derived cardiomyocytes showed increased nuclear area, decreased nuclear invagination, and increased half-decay time of the nuclear Ca2+ transient. An autopsied heart from a patient with EDMD also showed increased nuclear area and decreased nuclear invagination. These data suggest that Emerin plays a crucial role in nuclear structure and in the nuclear Ca2+ transient. Thus, emerin and the nuclear Ca2+ transient are possible therapeutic targets in heart failure and EDMD.
Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase
Authors: Choi Eun Sil , Min Kyoungseon , Kim Geun-Joong , Kwon Inchan , Kim Yong Hwan
Published: March 2017

Although aerobic CO dehydrogenases (CODHs) might be applicable in various fields, their practical applications have been hampered by low activity and no heterologous expression. We, for the first time, could functionally express recombinant PsCODH in E. coli and obtained a highly concentrated recombinant enzyme using an easy and convenient method. Its electron acceptor spectra, optimum conditions (pH 6.5 and 30 °C), and kinetic parameters (kcat of 12.97 s−1, Km of 0.065 mM, and specific activity of 0.86 Umg−1) were examined. Blast furnace gas (BFG) containing 20% CO, which is a waste gas from the steel-making process, was tested as a substrate for PsCODH. Even with BFG, the recombinant PsCODH retained 88.2% and 108.4% activity compared with those of pure CO and 20% CO, respectively. The results provide not only a promising strategy to utilize CO-containing industrial waste gases as cheap, abundant, and renewable resources but also significant information for further studies about cascade reactions producing value-added chemicals via CO2 as an intermediate produced by a CODH-based CO-utilization system, which would ultimately expand the versatility of CODH.
Tailored Fano resonance and localized electromagnetic field enhancement in Ag gratings
Authors: Li Zhaozhu , Klopf J. Michael , Wang Lei , Yang Kaida , Lukaszew Rosa A.
Published: March 2017

Metallic gratings can support Fano resonances when illuminated with EM radiation, and their characteristic reflectivity versus incident angle lineshape can be greatly affected by the surrounding dielectric environment and the grating geometry. By using conformal oblique incidence thin film deposition onto an optical grating substrate, it is possible to increase the grating amplitude due to shadowing effects, thereby enabling tailoring of the damping processes and electromagnetic field couplings of the Fano resonances, hence optimizing the associated localized electric field intensity. To investigate these effects we compare the optical reflectivity under resonance excitation in samples prepared by oblique angle deposition (OAD) and under normal deposition (ND) onto the same patterned surfaces. We observe that by applying OAD method, the sample exhibits a deeper and narrower reflectivity dip at resonance than that obtained under ND. This can be explained in terms of a lower damping of Fano resonance on obliquely deposited sample and leads to a stronger localized electric field. This approach opens a fabrication path for applications where tailoring the electromagnetic field induced by Fano resonance can improve the figure of merit of specific device characteristics, e.g. quantum efficiency (QE) in grating-based metallic photocathodes.
Cardiac Autonomic Alteration and Metabolic Syndrome: An Ambulatory ECG-based Study in A General Population
Authors: Ma Yan , Tseng Ping-Huei , Ahn Andrew , Wu Ming-Shiang , Ho Yi-Lwun , Chen Ming-Fong , Peng Chung-Kang
Published: March 2017

Metabolic syndrome (MetS) has been associated with chronic damage to the cardiovascular system. This study aimed to evaluate early stage cardiac autonomic dysfunction with electrocardiography (ECG)-based measures in MetS subjects. During 2012–2013, 175 subjects with MetS and 226 healthy controls underwent ECG recordings of at least 4 hours starting in the morning with ambulatory one-lead ECG monitors. MetS was diagnosed using the criteria defined in the Adult Treatment Panel III, with a modification of waist circumference for Asians. Conventional heart rate variability (HRV) analysis, and complexity index (CI1–20) calculated from 20 scales of entropy (multiscale entropy, MSE), were compared between subjects with MetS and controls. Compared with the healthy controls, subjects with MetS had significantly reduced HRV, including SDNN and pNN20 in time domain, VLF, LF and HF in frequency domain, as well as SD2 in Poincaré analysis. MetS subjects have significantly lower complexity index (CI1–20) than healthy subjects (1.69 ± 0.18 vs. 1.77 ± 0.12, p < 0.001). MetS severity was inversely associated with the CI1–20 (r = −0.27, p < 0.001). MetS is associated with significant alterations in heart rate dynamics, including HRV and complexity.
Late Quaternary faulting in the Sevier Desert driven by magmatism
Authors: Stahl T. , Niemi N. A.
Published: March 2017

Seismic hazard in continental rifts varies as a function of strain accommodation by tectonic or magmatic processes. The nature of faulting in the Sevier Desert, located in eastern Basin and Range of central Utah, and how this faulting relates to the Sevier Desert Detachment low-angle normal fault, have been debated for nearly four decades. Here, we show that the geodetic signal of extension across the eastern Sevier Desert is best explained by magma-assisted rifting associated with Plio-Pleistocene volcanism. GPS velocities from 14 continuous sites across the region are best-fit by interseismic strain accumulation on the southern Wasatch Fault at c. 3.4 mm yr−1 with a c. 0.5 mm yr−1 tensile dislocation opening in the eastern Sevier Desert. The characteristics of surface deformation from field surveys are consistent with dike-induced faulting and not with faults soling into an active detachment. Geologic extension rates of c. 0.6 mm yr−1 over the last c. 50 kyr in the eastern Sevier Desert are consistent with the rates estimated from the geodetic model. Together, these findings suggest that Plio-Pleistocene extension is not likely to have been accommodated by low-angle normal faulting on the Sevier Desert Detachment and is instead accomplished by strain localization in a zone of narrow, magma-assisted rifting.
CCL2 nitration is a negative regulator of chemokine-mediated inflammation
Authors: Barker Catriona E. , Thompson Sarah , O’Boyle Graeme , Lortat-Jacob Hugues , Sheerin Neil S. , Ali Simi , Kirby John A.
Published: March 2017

Chemokines promote leukocyte recruitment during inflammation. The oxidative burst is an important effector mechanism, this leads to the generation of reactive nitrogen species (RNS), including peroxynitrite (ONOO). The current study was performed to determine the potential for nitration to alter the chemical and biological properties of the prototypical CC chemokine, CCL2. Immunofluorescence was performed to assess the presence of RNS in kidney biopsies. Co-localisation was observed between RNS-modified tyrosine residues and the chemokine CCL2 in diseased kidneys. Nitration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p < 0.05). This was consistent with a trend towards reduced affinity of the nitrated chemokine for its cognate receptor CCR2b. The nitrated chemokine was unable to induce transendothelial monocyte migration in vitro and failed to promote leukocyte recruitment when added to murine air pouches (p < 0.05). This could potentially be attributed to reduced glycosaminoglycan binding ability, as surface plasmon resonance spectroscopy showed that nitration reduced heparan sulphate binding by CCL2. Importantly, intravenous administration of nitrated CCL2 also inhibited the normal recruitment of leukocytes to murine air pouches filled with unmodified CCL2. Together these data suggest that nitration of CCL2 during inflammation provides a mechanism to limit and resolve acute inflammation.
Extracellular matrix and α5β1 integrin signaling control the maintenance of bone formation capacity by human adipose-derived stromal cells
Authors: Di Maggio Nunzia , Martella Elisa , Frismantiene Agne , Resink Therese J. , Schreiner Simone , Lucarelli Enrico , Jaquiery Claude , Schaefer Dirk J. , Martin Ivan , Scherberich Arnaud
Published: March 2017

Stromal vascular fraction (SVF) cells of human adipose tissue have the capacity to generate osteogenic grafts with intrinsic vasculogenic properties. However, adipose-derived stromal/stem cells (ASC), even after minimal monolayer expansion, display poor osteogenic capacity in vivo. We investigated whether ASC bone-forming capacity may be maintained by culture within a self-produced extracellular matrix (ECM) that recapitulates the native environment. SVF cells expanded without passaging up to 28 days (Unpass-ASC) deposited a fibronectin-rich extracellular matrix and displayed greater clonogenicity and differentiation potential in vitro compared to ASC expanded only for 6 days (P0-ASC) or for 28 days with regular passaging (Pass-ASC). When implanted subcutaneously, Unpass-ASC produced bone tissue similarly to SVF cells, in contrast to P0- and Pass-ASC, which mainly formed fibrous tissue. Interestingly, clonogenic progenitors from native SVF and Unpass-ASC expressed low levels of the fibronectin receptor α5 integrin (CD49e), which was instead upregulated in P0- and Pass-ASC. Mechanistically, induced activation of α5β1 integrin in Unpass-ASC led to a significant loss of bone formation in vivo. This study shows that ECM and regulation of α5β1-integrin signaling preserve ASC progenitor properties, including bone tissue-forming capacity, during in vitro expansion.
Transparent Memory For Harsh Electronics
Authors: Ho C. H. , Retamal J. R. Durán , Yang P. K. , Lee C. P. , Tsai M. L. , Kang C. F. , He Jr-Hau
Published: March 2017

As a new class of non-volatile memory, resistive random access memory (RRAM) offers not only superior electronic characteristics, but also advanced functionalities, such as transparency and radiation hardness. However, the environmental tolerance of RRAM is material-dependent, and therefore the materials used must be chosen carefully in order to avoid instabilities and performance degradation caused by the detrimental effects arising from environmental gases and ionizing radiation. In this work, we demonstrate that AlN-based RRAM displays excellent performance and environmental stability, with no significant degradation to the resistance ratio over a 100-cycle endurance test. Moreover, transparent RRAM (TRRAM) based on AlN also performs reliably under four different harsh environmental conditions and 2 MeV proton irradiation fluences, ranging from 1011 to 1015 cm−2. These findings not only provide a guideline for TRRAM design, but also demonstrate the promising applicability of AlN TRRAM for future transparent harsh electronics.
Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding
Authors: Shahi Payam , Kim Samuel C. , Haliburton John R. , Gartner Zev J. , Abate Adam R.
Published: March 2017

Proteins are the primary effectors of cellular function, including cellular metabolism, structural dynamics, and information processing. However, quantitative characterization of proteins at the single-cell level is challenging due to the tiny amount of protein available. Here, we present Abseq, a method to detect and quantitate proteins in single cells at ultrahigh throughput. Like flow and mass cytometry, Abseq uses specific antibodies to detect epitopes of interest; however, unlike these methods, antibodies are labeled with sequence tags that can be read out with microfluidic barcoding and DNA sequencing. We demonstrate this novel approach by characterizing surface proteins of different cell types at the single-cell level and distinguishing between the cells by their protein expression profiles. DNA-tagged antibodies provide multiple advantages for profiling proteins in single cells, including the ability to amplify low-abundance tags to make them detectable with sequencing, to use molecular indices for quantitative results, and essentially limitless multiplexing.