bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal discharge planning for esophagectomy patients with enhanced recovery after surgery: Recommendations
Authors: Li Kunzhi , Wang Kangning , Wei Xing , Leng Xuefeng , Fang Qiang
Published: January 2023

Background

Studies have suggested that the postoperative length of stay (PLOS) of esophagectomy patients under the enhanced recovery after surgery (ERAS) pathway should be >10 days as against the previously recommended 7 days. We investigated the distribution and influencing factors of PLOS in the ERAS pathway in order to recommend an optimal planned discharge time.

Methods

This was a single-center retrospective study of 449 patients with thoracic esophageal carcinoma who underwent esophagectomy and perioperative ERAS between January 2013 and April 2021. We established a database to prospectively document the causes of delayed discharge.

Results

The mean and median PLOS were 10.2 days and 8.0 days (range: 5–97), respectively. Patients were divided into four groups: group A (PLOS ≤ 7 days), 179 patients (39.9%); group B (8 ≤ PLOS ≤ 10 days), 152 (33.9%); group C (11 ≤ PLOS ≤ 14 days), 68 (15.1%); group D (PLOS > 14 days), 50 patients (11.1%). The main cause of prolonged PLOS in group B was minor complications (prolonged chest drainage, pulmonary infection, recurrent laryngeal nerve injury). Severely prolonged PLOS in groups C and D were due to major complications and comorbidities. On multivariable logistic regression analysis, open surgery, surgical duration >240 min, age >64 years, surgical complication grade >2, and critical comorbidities were identified as risk factors for delayed discharge.

Conclusions

The optimal planned discharge time for patients undergoing esophagectomy with ERAS should be 7–10 days with a 4-day discharge observation window. Patients at risk of delayed discharge should be managed adopting PLOS prediction.
What can go wrong when observations are not independently and identically distributed: A cautionary note on calculating correlations on combined data sets from different experiments or conditions
Authors: Saccenti Edoardo
Published: January 2023

In the scientific literature data analysis results are often presented when samples from different experiments or different conditions, technical replicates or times series are merged to increase the sample size before calculating the correlation coefficient. This way of proceeding violates two basic assumptions underlying the use of the correlation coefficient: sampling from one population and independence of the observations (independence of errors). Since correlations are used to measure and infer associations between biological entities, this has tremendous implications on the reliability of scientific results, as the violation of these assumption leads to wrong and biased results. In this technical note, I review some basic properties of the Pearson’s correlation coefficient and illustrate some exemplary problems with simulated and experimental data, taking a didactic approach with the use of supporting graphical examples.
Pharmacokinetic and pharmacodynamic properties of pantoprazole in calves
Authors: Olivarez Jeff D. , Mulon Pierre-Yves , Ebner Lisa S. , Cremerius Haley , Cantrell Channing , Rahn Rebecca , Soto-Gonzalez Windy , Bergman Joan , Cox Sherry , Mochel Jonathan P. , Kreuder Amanda J. , Smith Joe S.
Published: January 2023

Introduction

Development of abomasal ulceration is a large concern, especially within calves; however, there is a paucity of research into the use of gastro protectants in ruminant species. Proton pump inhibitors, such as pantoprazole, are widely used in humans and companion animals. Their efficacy in ruminant species is undetermined. The objectives of this study were to 1) estimate the plasma pharmacokinetic parameters for pantoprazole in neonatal calves after three days of intravenous (IV) or subcutaneous (SC) administration, and 2) measure the effect pantoprazole had on abomasal pH over the treatment period.

Methods

Pantoprazole was administered to 6 Holstein-Angus cross bull calves at a dose of 1 mg/kg (IV) or 2 mg/kg (SC), once a day (every 24 h) for three days. Plasma samples were collected over a 72 h period and analyzed via HPLC-UV for determining pantoprazole concentrations. Pharmacokinetic parameters were derived via non-compartmental analysis. Abomasal (n= 8) samples were collected via abomasal cannulas over a 12 h period, per calf per day. Abomasal pH was determined via a bench top pH analyzer.

Results

Following Day 1 of IV administration, plasma clearance, elimination half-life, and volume of distribution of pantoprazole were estimated at 199.9 mL/kg/h, 1.44 h, and 0.51 L/kg, respectively. On Day 3 of IV administration, the reported values were 192.9 mL/kg/h, 2.52 h, and 1.80 L/kg mL, respectively. Elimination half-life and volume of distribution (V/F) of pantoprazole following SC administration were estimated at 1.81 h and 0.55 L/kg, respectively, on Day 1; and 2.99 h and 2.82 L/kg, respectively, on Day 3.

Discussion

The reported values for IV administration were similar to those previously reported in calves. SC administration appears to be well absorbed and tolerated. The sulfone metabolite was detectable for 36 h after the last administration for both routes. Abomasal pH was significantly higher than the pre-pantoprazole pH 4, 6, and 8 h after administration in both the IV and SC groups. Further studies of pantoprazole as a treatment/preventative for abomasal ulcers are warranted.
Evaluation of the role of clathrin and bacterial viability in the endocytosis of Lawsonia intracellularis
Authors: Pereira Carlos Eduardo Real , Resende Talita Pilar , Daniel Amanda Gabrielle de Souza , Vannucci Fabio Augusto , Gebhart Connie , Guedes Roberto Mauricio Carvalho
Published: January 2023

Lawsonia intracellularis is an obligate intracellular bacterium and causative agent of proliferative enteropathy. The pathogenesis of L. intracellularis is not completely understood, including the endocytic mechanisms to access the host cell cytoplasm. In this study, we evaluated the mechanisms involved in endocytosis of L. intracellularis in vitro using intestinal porcine epithelial cells (IPEC-J2). Confocal microscopy was used to co-localize L. intracellularis and clathrin. Clathrin gene knockdown was then applied to verify whether L. intracellularis endocytosis is clathrin-dependent. Finally, internalization of viable and non-viable (bacteria were inactivated by heat) L. intracellularis organisms were assessed to study the role of the host cell during bacterial endocytosis. L. intracellularis organisms were observed co-localized with clathrin by confocal microscopy but the amount of L. intracellularis internalized in cells, with and without clathrin knockdown, did not differ statistically. The internalization of non-viable L. intracellularis showed a decrease in the internalization in cells with less clathrin synthesis (P<0.05). The present study is the first to elucidate the involvement of clathrin in the endocytosis of L. intracellularis. Clathrin-mediated endocytosis was shown to be an important, but not required, process for L. intracellularis internalization in porcine intestinal epithelial cells. Independence of bacterial viability for host cell internalization was also confirmed.
Characterization and epidemiology of antimicrobial resistance patterns of Salmonella spp. and Staphylococcus spp. in free-ranging rhesus macaque (Macaca mulatta) at high-risk interfaces with people and livestock in Bangladesh
Authors: Rahman Md. Kaisar , Hassan Mohammad Mahmudul , Islam Shariful , Rostal Melinda K. , Uddin Md. Helal , Hagan Emily , Samad Mohammed Abdus , Flora Meerjady Sabrina , Epstein Jonathan H. , Islam Ariful
Published: January 2023

Introduction

Antimicrobial resistance (AMR) is a growing global health threat for humans and animals. Environmental contamination of antimicrobials from human and domestic animal feces has been linked to AMR in wildlife populations, including rhesus macaques. This study aimed to describe the eco-epidemiology of AMR within Salmonella and Staphylococcus species isolated from rhesus macaques.

Methods

We followed macaque groups for 4 h per day (2 days) to observe the direct and indirect contact rate and type between macaques and people and livestock. We collected 399 freshly defecated, non-invasive fecal samples from macaques at seven sites in Bangladesh in January–June 2017. Bacterial isolation and identification were conducted using culture, biochemical characteristics, and polymerase chain reaction (PCR). An antimicrobial susceptibility test (AST) for 12 antimicrobials for each organism was conducted using the Kirby–Bauer disc diffusion method.

Results

The overall prevalence of Salmonella spp. and Staphylococcus spp. in rhesus macaques was 5% (n = 18; 95% CI: 3–7%) and 16% (n = 64; 95% CI: 13–20%), respectively. All the isolated Salmonella spp. and most of the Staphylococcus spp. (95%; 61/64; 95% CI: 86.9–99%) were resistant to at least one antimicrobial. The odds of a fecal sample having antimicrobial-resistant Salmonella spp (OR = 6.6; CI: 0.9–45.8, P = 0.05) and Staphylococcus spp. (OR = 5.6; CI: 1.2–26, P = 0.02) were significantly higher in samples collected at peri-urban sites than those collected at rural and urban sites. Salmonella spp. were most frequently resistant to tetracycline (89%), azithromycin (83%), sulfamethoxazole-trimethoprim (50%), and nalidixic acid (44%). Staphylococcus spp. were found to be highly resistant to ampicillin (93%), methicillin (31%), clindamycin (26%), and rifampicin (18%). Both bacterial species produced colonies with multidrug resistance to up to seven antimicrobials. Direct and indirect contact rates (within 20 m for at least 15 min) and resource sharing between macaques and people were higher in urban sites, while macaque-livestock contact rates were higher in rural sites.

Discussion

The study shows that resistant microorganisms are circulating in rhesus macaque, and direct and indirect contact with humans and livestock might expand the resistant organisms.
Co-circulation of dengue virus serotypes 1 and 3 in the Fatick region of senegal 2018
Authors: Diagne Cheikh Tidiane , Dieng Idrissa , Faye Oumar , Fall Cheikh , Barry Mamadou Aliou , Diarra Maryam , Ndiaye Oumar , Dior Ndione Marie Henriette , Ndiaye Mamadou , Diop Boly , Bousso Abdoulaye , Sall Abiboulaye , Fall Gamou , Loucoubar Cheikh , Ba Yamar , Sall Amadou Alpha , Diallo Mawlouth , Faye Ousmane
Published: January 2023

On 10th September 2018, the Syndromic Sentinel Surveillance network that monitors febrile illnesses in all 14 regions of Senegal detected a peak of fever in the Fatick region. On 13 September 2018, 10 samples were sent to the WHO Collaborating Centre for Arboviruses and Viral Haemorrhagic Fevers at the Institut Pasteur de Dakar (IPD). Laboratory investigations revealed an epidemic of dengue 1 genotype V and dengue 3 genotype III. Fatick neighbors the Holy City of Touba where 3.5 million people from all over the word gather every year for the Grand Magal pilgrimage. This article discusses the impact of mass gatherings and their role in the recent introduction of dengue serotypes in Senegal. Dengue is now endemic in Senegal and across many countries in Africa, highlighting the need for early detection, control measures and prevention of severe dengue cases in highly connected urban settings.
Patients With Systemic Reaction to Their Hernia Mesh: An Introduction to Mesh Implant Illness
Authors: Fadaee Negin , Huynh Desmond , Khanmohammed Zayan , Mazer Laura , Capati Isabel , Towfigh Shirin
Published: January 2023

In our practice, we have noticed an increased number of patients requiring mesh removal due to a systemic reaction to their implant. We present our experience in diagnosing and treating a subpopulation of patients who require mesh removal due to a possible mesh implant illness (MII). All patients who underwent mesh removal for indication of mesh reaction were captured from a hernia database. Data extraction focused on the patients’ predisposing medical conditions, presenting symptoms suggestive of mesh implant illness, types of implants to which reaction occurred, and postoperative outcome after mesh removal. Over almost 7 years, 165 patients had mesh removed. Indication for mesh removal was probable MII in 28 (17%). Most were in females (60%), average age was 46 years, with average pre-operative pain score 5.4/10. All patients underwent complete mesh removal. Sixteen (57%) required tissue repair of their hernia; 4 (14%) had hybrid mesh implanted. Nineteen (68%) had improvement and/or resolution of their MII symptoms within the first month after removal. We present insight into a unique but rising incidence of patients who suffer from systemic reaction following mesh implantation. Predisposing factors include female sex, history of autoimmune disorder, and multiple medical and environmental allergies and sensitivities. Presenting symptoms included spontaneous rashes, erythema and edema over the area of implant, arthralgia, headaches, and chronic fatigue. Long-term follow up after mesh removal confirmed resolution of symptoms after mesh removal. We hope this provides greater attention to patients who present with vague, non-specific but debilitating symptoms after mesh implantation.
Design and Experimental Characterization of Artificial Neural Network Controller for a Lower Limb Robotic Exoskeleton
Authors: Lin Chih-Jer , Sie Ting-Yi
Published: January 2023

This study aims to develop a lower limb robotic exoskeleton with the use of artificial neural networks for the purpose of rehabilitation. First, the PID control with iterative learning controller is used to test the proposed lower limb robotic exoskeleton robot (LLRER). Although the hip part using the flat brushless DC motors actuation has good tracking results, the knee part using the pneumatic actuated muscle (PAM) actuation cannot perform very well. Second, to compensate this nonlinearity of PAM actuation, the artificial neural network (ANN) feedforward control based on the inverse model trained in advance are used to compensate the nonlinearity of the PAM. Third, a particle swarm optimization (PSO) is used to optimize the PID parameters based on the ANN-feedforward architecture. The developed controller can complete the tracking of one gait cycle within 3.6 s for the knee joint. Among the three controllers, the controller of the ANN-feedforward with PID control (PSO tuned) performs the best, even when the LLRER is worn by the user and the tracking performance is still very good. The average Mean Absolute Error (MAE) of the left knee joint is 1.658 degrees and the average MAE of the right knee joint is 1.392 degrees. In the rehabilitation tests, the controller of ANN-feedforward with PID control is found to be suitable and its versatility for different walking gaits is verified during human tests. The establishment of its inverse model does not need to use complex mathematical formulas and parameters for modeling. Moreover, this study introduces the PSO to search for the optimal parameters of the PID. The architecture diagram and the control signal given by the ANN compensation with the PID control can reduce the error very well.
Iterative Feedback Tuning of Model-Free Intelligent PID Controllers
Authors: Baciu Andrei , Lazar Corneliu
Published: January 2023

In the last decades, model-free control (MFC) has become an alternative for complex processes whose models are not available or are difficult to obtain. Among the model-free control techniques, intelligent PID (iPID) algorithms, which are based on the ultralocal model parameterized with the constant α and including a classical PID, are used in many applications. This paper presents a new method for tuning iPID controllers based on the iterative feedback tuning (IFT) technique. This model-free tuning technique iteratively optimizes the parameters of a fixed structure controller using data coming from the closed-loop system operation. First, the discrete transfer functions of the iPID are deduced, considering the first and second order derivatives of the output variable from the ultralocal model. Using the discrete transfer functions, the iPID controllers become the fixed structure type, and the IFT parameter tuning method can be applied. Thus, in addition to the classical gains of the PID algorithm, the value of the parameter α is also obtained, which is usually determined by trial-and-error. The performances of the IFT-tuned iPID controllers were experimentally tested and validated in real-time using Quanser AERO 2 laboratory equipment with a one degree of freedom (1-DOF) configuration.
A Comparative Study of Optimization Models for Condition-Based Maintenance Scheduling of an Aircraft Fleet
Authors: Tseremoglou Iordanis , van Kessel Paul J. , Santos Bruno F.
Published: January 2023

Condition-based maintenance (CBM) scheduling of an aircraft fleet in a disruptive environment while considering health prognostics for a set of systems is a very complex combinatorial problem, which is becoming more challenging in light of the uncertainty included in health prognostics. This type of problem falls under the broad category of resource-constrained scheduling problems under uncertainty and is often solved using a mixed integer linear programming (MILP) formulation. While a MILP framework is very promising, the problem size can scale exponentially with the number of considered aircraft and considered tasks, leading to significantly high computational costs. The most recent advances in artificial intelligence have demonstrated the capability of deep reinforcement learning (DRL) algorithms to alleviate this curse of dimensionality, as once the DRL agent is trained, it can achieve real-time optimization of the maintenance schedule. However, there is no guarantee of optimality. These comparative merits of a MILP and a DRL formulation for the aircraft fleet maintenance scheduling problem have not been discussed in the literature. This study is a response to this research gap. We conduct a comparison of a MILP and a DRL scheduling model, which are used to derive the optimal maintenance schedule for various maintenance scenarios for aircraft fleets of different sizes in a disruptive environment, while considering health prognostics and the available resources for the execution of each task. The quality of solutions is evaluated on the basis of four planning objectives, defined according to real airline practice. The results show that the DRL approach achieves better results with respect to scheduling of prognostics-driven tasks and requires less computational time, whereas the MILP model produces more stable maintenance schedules and induces less maintenance ground time. Overall, the comparison provides valuable insights for the integration of health prognostics in airline maintenance practice.
Fast Inverse Design of Transonic Airfoils by Combining Deep Learning and Efficient Global Optimization
Authors: Deng Feng , Yi Jianmiao
Published: January 2023

In this paper, a deep learning model trained to generate well-posed pressure distributions at transonic speeds is coupled by the efficient global optimization (EGO) algorithm to speed up the inverse design process for transonic airfoils. First, the Wasserstein generative adversarial network (WGAN) is trained to generate well-posed pressure distributions at transonic speeds. Then, the EGO algorithm is used to pick up a pressure distribution in WGAN by solving the associated optimization problem defined for matching the prescribed pressure features, such as the suction peak and the shock-wave position. Finally, a deep convolutional neural network (DCNN) for nonlinear mapping is adopted to obtain the corresponding airfoil shape. Several cases with prescribed pressure features were performed to verify the feasibility and efficiency of the proposed method. Test cases indicate that the airfoil shape with the desired pressure distribution can be found in around one minute using a desktop computer with an Intel i5-9300H CPU.
DNS Study on Turbulent Transition Induced by an Interaction between Freestream Turbulence and Cylindrical Roughness in Swept Flat-Plate Boundary Layer
Authors: Nakagawa Kosuke , Tsukahara Takahiro , Ishida Takahiro
Published: January 2023

Laminar-to-turbulent transition in a swept flat-plate boundary layer is caused by the breakdown of the crossflow vortex via high-frequency secondary instability and is promoted by the wall-surface roughness and the freestream turbulence (FST). Although the FST is characterized by its intensity and wavelength, it is not clear how the wavelength affects turbulent transitions and interacts with the roughness-induced transition. The wavelength of the FST depends on the wind tunnel or in-flight conditions, and its arbitrary control is practically difficult in experiments. By means of direct numerical simulation, we performed a parametric study on the interaction between the roughness-induced disturbance and FST in the Falkner–Skan–Cooke boundary layer. One of our aims is to determine the critical roughness height and its dependence on the turbulent intensity and peak wavelength of FST. We found a suppression and promotion in the transition process as a result of the interaction. In particular, the immediate transition behind the roughness was delayed by the long-wavelength FST, where the presence of FST suppressed the high-frequency disturbance emanating from the roughness edge. Even below the criticality, the short-wavelength FST promoted a secondary instability in the form of the hairpin vortex and triggered an early transition before the crossflow-vortex breakdown with the finger vortex. Thresholds for the FST wavelengths that promote or suppress the early transition were also discussed to provide a practically important indicator in the prediction and control of turbulent transitions due to FST and/or roughness on the swept wing.
Different Mechanized Fertilization Methods in Nutrient Utilization and Summer Maize Yield
Authors: Yuan Wensheng , Wang Yilun , Jin Chengqian , Feng Yugang , Zhang Longmei , Hu Yu
Published: January 2023

In the production of summer maize, the problems of excessive fertilizer input and low fertilizer utilization rate are common, resulting in the waste of resources and environmental pollution. In order to explore the optimal fertilization mode of summer maize, a field experiment with eight treatments was designed in which sowing and fertilization were carried out by different machines. The effects of the two fertilization methods, i.e., hole fertilization and strip fertilization; two fertilization positions, i.e., side-position fertilization and positive-position fertilization; and two fertilization depths, i.e., 10 cm and 15 cm, on the summer maize yield, plant nitrogen, phosphorus, and potassium nutrient accumulation, and aboveground biomass were studied. The results show that different fertilization modes had significant effects on summer maize yield, nitrogen, phosphorus, and potassium nutrient accumulation, and aboveground biomass. In general, when the strip fertilization method was used, 15 cm deep positive-position fertilization was superior. When the hole fertilization method was used, 15 cm deep side-position fertilization was superior.
Partition Management of Soil Nutrients Based on Capacitive Coupled Contactless Conductivity Detection
Authors: Wei Yuanyuan , Wang Rujing , Zhang Junqing , Guo Hongyan , Chen Xiangyu
Published: January 2023

A method based on capacitively coupled contactless conductivity detection (C4D), which has been proven effective for the rapid detection of available soil potassium content, was firstly proposed to apply to soil nutrient detection. By combining a detection signal spectrum analysis, geographic information system (GIS) data, and a cluster analysis, a soil nutrient management system to match the detection device was developed. This system included six modules: soil sample information management, electrophoresis analysis, quantitative calculation, nutrient result viewing, cluster analysis, and nutrient distribution map generation. The soil samples, which were collected from an experimental field in Xuchang City of Henan Province, were analyzed using the C4D and flame photometer methods. The results showed that the detection results for the soil samples obtained via the two methods were in good agreement. C4D technology was feasible for the detection of the soil available nutrients and had the advantages of a high timeliness, low sample volume, and low pollution. The soil nutrient management system adopted the hierarchical clustering method to classify the grid cells of the experimental field according to the nutrient detection results. A soil nutrient distribution map displayed the spatial difference in nutrients. This paper provides a systematic solution for soil nutrient zone management that includes nutrient detection, signal analysis, data management for the nutrient zone, and field nutrient distribution map generation to support decision making in variable fertilization.
Genetic Polymorphism and mRNA Expression Studies Reveal IL6R and LEPR Gene Associations with Reproductive Traits in Chinese Holsteins
Authors: Zhang Hailiang , Sammad Abdul , Shi Rui , Dong Yixin , Zhao Shanjiang , Liu Lin , Guo Gang , Xu Qing , Liu Aoxing , Wang Yachun
Published: January 2023

Genetic selection of milk yield traits alters the energy distribution of high producing cows, resulting in gene-induced negative energy balance, and consequently, poor body condition scores and reduced reproductive performances. Here, we investigated two metabolic-syndrome pathway genes, IL6R (Interleukin 6 receptor) and LEPR (Leptin receptor), for their polymorphism effects on reproductive performance in dairy cows, by applying polymorphism association analyses in 1588 Chinese Holstein cows (at population level) and gene expression analyses in granulosa cells isolated from eight cows (at cell level). Among the six single nucleotide polymorphisms we examined (two SNPs for IL6R and four SNPs for LEPR), five were significantly associated with at least one reproductive trait, including female fertility traits covering both the ability to recycle after calving and the ability to conceive and keep pregnancy when inseminated properly, as well as calving traits. Notably, the identified variant SNP g.80143337A/C in LEPR is a missense variant. The role of IL6R and LEPR in cattle reproduction were further confirmed by observed differences in relative gene expression levels amongst granulosa cells with different developmental stages. Collectively, the functional validation of IL6R and LEPR performed in this study improved our understanding of cattle reproduction while providing important molecular markers for genetic selection of reproductive traits in high-yielding dairy cattle.
Effects of Energy Crop Monocultures and Sewage Sludge Fertiliser on Soils and Earthworm Community Attributes
Authors: Mazur-Pączka Anna , Pączka Grzegorz , Garczyńska Mariola , Jaromin Marcin , Hajduk Edmund , Kostecka Joanna , Butt Kevin R.
Published: January 2023

Biomass is one of the most significant renewable energy sources. Doubts arise from large-area plantations of energy monocultures, which can lead to the depletion of, and a decrease in, soil biodiversity. Community association analyses of Lumbricidae may help to indicate environmental change. Therefore, the study objectives were to determine the qualitative and quantitative diversity of Lumbricids in plantations of energy crops—basket willow (Salix viminalis L.), foxglove tree (Paulownia tomentosa Steud.), and black locust (Robinia pseudoacacia L.)—by investigating the following cultivation treatments: SV and SVSS—S. viminalis without (w/o) and with the addition of sewage sludge to the soil (+SS); PT and PTSS—P. tomentosaw/o and + SS; RP and RPSS—R. pseudoacaciaw/o and +SS; and MW—meadow community. A significantly higher density (p < 0.05) and biomass (p < 0.05) of earthworms were found in the SV and SVSS plantations than in other sites. The application of sewage sludge contributed to a significant increase in the mean number and biomass of all Lumbricids within cultivations of S. viminalis and R. pseudoacacia, which were significantly higher than those in the control site. This work suggests that woody energy crop production with an appropriate selection of plants and fertilisation can be a favourable habitat for the development of earthworm populations.
Geographical Indications and Risks of Unsustainability Linked to “Disaffection Effects” in the Dairy Sector
Authors: De Rosa Marcello , Masi Margherita , Apostolico Ludovica , Bartoli Luca , Francescone Martina
Published: January 2023

This paper deals with the role of geographical indications (GI) in the dairy sector of Italy, with the purpose of highlighting eventual negative dynamics in the adhesion to the GI by the potential operators of Italy. A negative adhesion to the GI circuit shows a “disaffection effect,” and has relevant implications on sustainability on account of the role of a GI in boosting various dimensions of sustainability (economic, social, environmental). In order to verify the presence of a disaffection effect, an empirical analysis of the actors adhering to the GI dairy sector is carried out. Through the collection of secondary sources from official databases, the paper emphasises a negative trend in the adhesion to the GI dairy sector, which also reveals diversified territorial impacts. More precisely, unlike other sectors, the dairy sector reveals negative dynamics in the operators choosing to adhere to the GI circuits. The results of the analysis address some policy issues and solicit policy action to limit the disaffection effect on GI in the dairy sector.
Droplet Penetration Model Based on Canopy Porosity for Spraying Applications
Authors: Ru Yu , Hu Chenming , Chen Xuyang , Yang Fengbo , Zhang Chao , Li Jianping , Fang Shuping
Published: January 2023

Analysing the penetration and droplet deposition characteristics in the canopy of fruit trees is critical for optimising the operational parameters of air-assisted spraying equipment, achieving precise application of chemicals, and improving the effectiveness of fruit tree pest and disease control. We used a mobile LIDAR system to detect the tree canopy characteristics and optical porosity and conduct wind tunnel experiments to investigate the interaction between the tree canopy, the airflow field, and the droplet penetration ratio in the canopy of fruit trees. The results show that the relative wind velocity decreases rapidly during canopy penetration, and that the minimum value occurs at the back of the canopy. The smaller the optical porosity, the greater the reduction in wind velocity is. The quadratic exponential regression model had the highest coefficient of determination (R2) (0.9672) and the lowest root mean square error (RMSE) (5.56%). This paper provides information on optimising the spraying parameters, improving the pesticide utilisation rate, and selecting the optimum spraying conditions and application parameters.
Giving a Hand: Synthetic Peptides Boost the Antifungal Activity of Itraconazole against Cryptococcus neoformans
Authors: Aguiar Tawanny K. B. , Feitosa Ricardo M. , Neto Nilton A. S. , Malveira Ellen A. , Gomes Francisco I. R. , Costa Ana C. M. , Freitas Cleverson D. T. , Mesquita Felipe P. , Souza Pedro F. N.
Published: January 2023

Cryptococcus neoformans is a multidrug-resistant pathogen responsible for infections in immunocompromised patients. Here, itraconazole (ITR), a commercial antifungal drug with low effectiveness against C. neoformans, was combined with different synthetic antimicrobial peptides (SAMPs), Mo-CBP3-PepII, RcAlb-PepII, RcAlb-PepIII, PepGAT, and PepKAA. The Mo-CBP3-PepII was designed based on the sequence of MoCBP3, purified from Moringa oleifera seeds. RcAlb-PepII and RcAlb-PepIII were designed using Rc-2S-Alb, purified from Ricinus communis seed cakes. The putative sequence of a chitinase from Arabidopsis thaliana was used to design PepGAT and PepKAA. All SAMPs have a positive liquid charge and a hydrophobic potential ranging from 41–65%. The mechanisms of action responsible for the combined effect were evaluated for the best combinations using fluorescence microscopy (FM). The synthetic peptides enhanced the activity of ITR by 10-fold against C. neoformans. Our results demonstrated that the combinations could induce pore formation in the membrane and the overaccumulation of ROS on C. neoformans cells. Our findings indicate that our peptides successfully potentialize the activity of ITR against C. neoformans. Therefore, synthetic peptides are potential molecules to assist antifungal agents in treating Cryptococcal infections.
Diagnosis by Microbial Culture, Breath Tests and Urinary Excretion Tests, and Treatments of Small Intestinal Bacterial Overgrowth
Authors: Maeda Yorinobu , Murakami Teruo
Published: January 2023

Small intestinal bacterial overgrowth (SIBO) is characterized as the increase in the number and/or alteration in the type of bacteria in the upper gastrointestinal tract and accompanies various bowel symptoms such as abdominal pain, bloating, gases, diarrhea, and so on. Clinically, SIBO is diagnosed by microbial culture in duodenum/jejunum fluid aspirates and/or the breath tests (BT) of hydrogen/methane gases after ingestion of carbohydrates such as glucose. The cultural analysis of aspirates is regarded as the golden standard for the diagnosis of SIBO; however, this is invasive and is not without risk to the patients. BT is an inexpensive and safe diagnostic test but lacks diagnostic sensitivity and specificity depending on the disease states of patients. Additionally, the urinary excretion tests are used for the SIBO diagnosis using chemically synthesized bile acid conjugates such as cholic acid (CA) conjugated with para-aminobenzoic acid (PABA-CA), ursodeoxycholic acid (UDCA) conjugated with PABA (PABA-UDCA) or conjugated with 5-aminosalicylic acid (5-ASA-UDCA). These conjugates are split by bacterial bile acid (cholylglycine) hydrolase. In the tests, the time courses of the urinary excretion rates of PABA or 5-ASA, including their metabolites, are determined as the measure of hydrolytic activity of intestinal bacteria. Although the number of clinical trials with this urinary excretion tests is small, results demonstrated the usefulness of bile acid conjugates as SIBO diagnostic substrates. PABA-UDCA disulfate, a single-pass type unabsorbable compound without the hydrolysis of conjugates, was likely to offer a simple and rapid method for the evaluation of SIBO without the use of radioisotopes or expensive special apparatus. Treatments of SIBO with antibiotics, probiotics, therapeutic diets, herbal medicines, and/or fecal microbiota transplantation are also reviewed.
Anti-Staphylococcal Activities of Rosmarinus officinalis and Myrtus communis Essential Oils through ROS-Mediated Oxidative Stress
Authors: Bowbe Khadijetou Hamoud , Salah Karima Bel Hadj , Moumni Sarra , Ashkan Mada F. , Merghni Abderrahmen
Published: January 2023

Rosmarinus officinalis and Myrtus communis essential oils (EOs) are well-known for their ethno-pharmaceutical properties. In the present study, we have analyzed the chemical composition of both EOs by gas chromatography-mass spectrometry. Then we assessed their antibacterial, antibiofilm, and anti-virulence actions against the opportunistic pathogen Staphylococcus aureus. The cytotoxic effect of agents tested against this bacterium was investigated by monitoring reactive oxygen-species (ROS) generation and antioxidant-enzyme (catalase) production. Regarding the antistaphylococcal effects, our results showed antibacterial efficacy of both Eos and their combination, where the minimum inhibitory concentrations ranged between 0.7 and 11.25 mg/mL. A combination of tested agents showed the highest anti-hemolytic and anti-protease effects. Additionally, association between EOs displayed more potency against the development of biofilm performed by S. aureus, with percentage of removal reaching 74%. The inhibitory impacts of EOs on S. aureus virulence factors were discovered to be concentration-dependent. Furthermore, our results provide insight on the abilities of R. officinalis and M. communis EOs, as well as their potential in combination, to generate ROS and affect oxidative stress enzyme catalase in S. aureus, leading to their antagonistic effect against this pathogen.
Neuroprotective Potentials of Flavonoids: Experimental Studies and Mechanisms of Action
Authors: Bellavite Paolo
Published: January 2023

Neurological and neurodegenerative diseases, particularly those related to aging, are on the rise, but drug therapies are rarely curative. Functional disorders and the organic degeneration of nervous tissue often have complex causes, in which phenomena of oxidative stress, inflammation and cytotoxicity are intertwined. For these reasons, the search for natural substances that can slow down or counteract these pathologies has increased rapidly over the last two decades. In this paper, studies on the neuroprotective effects of flavonoids (especially the two most widely used, hesperidin and quercetin) on animal models of depression, neurotoxicity, Alzheimer’s disease (AD) and Parkinson’s disease are reviewed. The literature on these topics amounts to a few hundred publications on in vitro and in vivo models (notably in rodents) and provides us with a very detailed picture of the action mechanisms and targets of these substances. These include the decrease in enzymes that produce reactive oxygen and ferroptosis, the inhibition of mono-amine oxidases, the stimulation of the Nrf2/ARE system, the induction of brain-derived neurotrophic factor production and, in the case of AD, the prevention of amyloid-beta aggregation. The inhibition of neuroinflammatory processes has been documented as a decrease in cytokine formation (mainly TNF-alpha and IL-1beta) by microglia and astrocytes, by modulating a number of regulatory proteins such as Nf-kB and NLRP3/inflammasome. Although clinical trials on humans are still scarce, preclinical studies allow us to consider hesperidin, quercetin, and other flavonoids as very interesting and safe dietary molecules to be further investigated as complementary treatments in order to prevent neurodegenerative diseases or to moderate their deleterious effects.
Dietary Mg Supplementation Decreases Oxidative Stress, Inflammation, and Vascular Dysfunction in an Experimental Model of Metabolic Syndrome with Renal Failure
Authors: López-Baltanás Rodrigo , Rodríguez-Ortiz María E. , Díaz-Tocados Juan M. , Martinez-Moreno Julio M. , Membrives Cristina , Rodelo-Haad Cristian , Pendón Ruiz de Mier M. Victoria , Rodríguez Mariano , Canalejo Antonio , Almadén Yolanda , Muñoz-Castañeda Juan R.
Published: January 2023

Background: Metabolic syndrome (MetS) and chronic kidney disease (CKD) are commonly associated with cardiovascular disease (CVD) and in these patients Mg concentration is usually decreased. This study evaluated whether a dietary Mg supplementation might attenuate vascular dysfunction through the modulation of oxidative stress and inflammation in concurrent MetS and CKD. Methods: A rat model of MetS (Zucker strain) with CKD (5/6 nephrectomy, Nx) was used. Nephrectomized animals were fed a normal 0.1%Mg (MetS+Nx+Mg0.1%) or a supplemented 0.6%Mg (MetS+Nx+Mg0.6%) diet; Sham-operated rats with MetS receiving 0.1%Mg were used as controls. Results: As compared to controls, the MetS+Nx-Mg0.1% group showed a significant increase in oxidative stress and inflammation biomarkers (lipid peroxidation and aortic interleukin-1b and -6 expression) and Endothelin-1 levels, a decrease in nitric oxide and a worsening in uremia and MetS associated pathology as hypertension, and abnormal glucose and lipid profile. Moreover, proteomic evaluation revealed changes mainly related to lipid metabolism and CVD markers. By contrast, in the MetS+Nx+Mg0.6% group, these parameters remained largely similar to controls. Conclusion: In concurrent MetS and CKD, dietary Mg supplementation reduced inflammation and oxidative stress and improved vascular function.
Vitamin and Carotenoid Intake and Outcomes of In Vitro Fertilization in Women Referring to an Italian Fertility Service: A Cross-Sectional Analysis of a Prospective Cohort Study
Authors: De Cosmi Valentina , Cipriani Sonia , Esposito Giovanna , Fedele Francesco , La Vecchia Irene , Trojano Giuseppe , Parazzini Fabio , Somigliana Edgardo , Agostoni Carlo
Published: January 2023

Background: Nutrition may impact reproductive health and fertility potential. The role of dietary antioxidants in affecting conception and birth outcomes is a topic of emerging interest. Methods: This cross-sectional analysis from a prospective cohort study aims to explore the relationship between the intake of antioxidants, vitamins, and carotenoids and the outcomes of assisted reproduction techniques. Information on the socio-demographic characteristics, health histories, lifestyle habits, and diet information of subfertile couples referred to a fertility center was obtained. Results: A total of 494 women were enrolled. According to the four IVF outcomes considered, 95% of women achieved good quality oocytes, 87% achieved embryo transfer, 32.0% achieved clinical pregnancies, and 24.5% achieved pregnancy at term. Associations were found between age and the number of good quality oocytes (p = 0.02). A moderate level of physical activity in the prior 5 years was associated with a better rate of achieving clinical pregnancy (p = 0.03). Smoking habits, alcohol intake, and caffeine consumption did not show associations with any outcome. No associations were found, even after accounting for potential confounders, with the intake of vitamins C, D, E, and α-carotene, β-carotene, beta-cryptoxanthin, lutein, and folate. Conclusion: Further research is needed to understand how antioxidant intake may have a role in modulating fertility.
Comparative Transcriptomics Reveal Metabolic Rather than Genetic Control of Divergent Antioxidant Metabolism in the Primary Root Elongation Zone of Water-Stressed Cotton and Maize
Authors: Kang Jian , Sen Sidharth , Oliver Melvin J. , Sharp Robert E.
Published: January 2023

Under water stress, the primary root elongation zones of cotton and maize exhibit both conserved and divergent metabolic responses, including variations in sulfur and antioxidant metabolism. To explore the relative importance of metabolic and genetic controls of these responses for each species, and the extent to which responses are mediated by similar gene expression networks within the framework of ortholog groups, comparative transcriptomics analyses were conducted under conditions of equivalent tissue water stress. Ortholog analysis revealed that 86% of the transcriptome response to water stress was phylogenetically unrelated between cotton and maize. Elevated transcript abundances for genes involved in abscisic acid (ABA) biosynthesis and signaling, as well as key enzymes that enable osmotic adjustment, were conserved between the species. In contrast, antioxidant responses, at least with regard to glutathione metabolism and anti-oxidative enzymes, did not exhibit such a transcript abundance adaptive signature. In particular, previously characterized differential responses of the glutathione and sulfur metabolic pathways between cotton and maize were not evident in the transcriptomic responses. The findings indicate that the antioxidant response in both species results from a metabolic acclimation to water stress, and thus represents an example of water stress-related metabolic plasticity.
An Updated Overview on the Role of Small Molecules and Natural Compounds in the “Young Science” of Rejuvenation
Authors: Ribaudo Giovanni , Gianoncelli Alessandra
Published: January 2023

Aging is a gradual process that occurs over time which leads to a progressive decline of cells and tissues. Telomere shortening, genetic instability, epigenetic alteration, and the accumulation of misfolded proteins represent the main hallmarks that cause perturbed cellular functions; this occurs in conjunction with the progression of the so-called “aging clocks”. Rejuvenation aims to influence the natural evolution of such aging clocks and to enhance regenerative capacity, thus overcoming the limitations of common anti-aging interventions. Current rejuvenation processes are based on heterochronic parabiosis, cell damage dilution through asymmetrical cell division, the excretion of extracellular vesicles, the modulation of genetic instability involving G-quadruplexes and DNA methylation, and cell reprogramming using Yamanaka factors and the actions of antioxidant species. In this context, we reviewed the most recent contributions that report on small molecules acting as senotherapeutics; these molecules act by promoting one or more of the abovementioned processes. Candidate drugs and natural compounds that are being studied as potential rejuvenation therapies act by interfering with CDGSH iron-sulfur domain 2 (CISD2) expression, G-quadruplex structures, DNA methylation, and mitochondrial decay. Moreover, direct and indirect antioxidants have been reported to counteract or revert aging through a combination of mixed mechanisms.
Novel Insights into the Role of the Antioxidants in Prostate Pathology
Authors: Rago Vittoria , Di Agostino Silvia
Published: January 2023

To date, it is known that antioxidants protect cells from damage caused by oxidative stress and associated with pathological conditions. Several studies have established that inflammation is a state that anticipates the neoplastic transformation of the prostate. Although many experimental and clinical data have indicated the efficacy of antioxidants in preventing this form of cancer, the discrepant results, especially from recent large-scale randomized clinical trials, make it difficult to establish a real role for antioxidants in prostate tumor. Despite these concerns, clinical efficacy and safety data show that some antioxidants still hold promise for prostate cancer chemoprevention. Although more studies are needed, in this review, we briefly describe the most common antioxidants that have shown benefits in preclinical and clinical settings, focusing our attention on synthesizing the advances made so far in prostate cancer chemoprevention using antioxidants as interesting molecules for the challenges of future therapies.
Protective Effects of Early Caffeine Administration in Hyperoxia-Induced Neurotoxicity in the Juvenile Rat
Authors: Heise Julia , Schmitz Thomas , Bührer Christoph , Endesfelder Stefanie
Published: January 2023

High-risk preterm infants are affected by a higher incidence of cognitive developmental deficits due to the unavoidable risk factor of oxygen toxicity. Caffeine is known to have a protective effect in preventing bronchopulmonary dysplasia associated with improved neurologic outcomes, although very early initiation of therapy is controversial. In this study, we used newborn rats in an oxygen injury model to test the hypothesis that near-birth caffeine administration modulates neuronal maturation and differentiation in the hippocampus of the developing brain. For this purpose, newborn Wistar rats were exposed to 21% or 80% oxygen on the day of birth for 3 or 5 days and treated with vehicle or caffeine (10 mg/kg/48 h). Postnatal exposure to 80% oxygen resulted in a drastic reduction of associated neuronal mediators for radial glia, mitotic/postmitotic neurons, and impaired cell-cycle regulation, predominantly persistent even after recovery to room air until postnatal day 15. Systemic caffeine administration significantly counteracted the effects of oxygen insult on neuronal maturation in the hippocampus. Interestingly, under normoxia, caffeine inhibited the transcription of neuronal mediators of maturing and mature neurons. The early administration of caffeine modulated hyperoxia-induced decreased neurogenesis in the hippocampus and showed neuroprotective properties in the neonatal rat oxygen toxicity model.