bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapping Wheat Dry Matter and Nitrogen Content Dynamics and Estimation of Wheat Yield Using UAV Multispectral Imagery Machine Learning and a Variety-Based Approach: Case Study of Morocco
Authors: Astaoui Ghizlane , Dadaiss Jamal Eddine , Sebari Imane , Benmansour Samir , Mohamed Ettarid
Published: January 2021

Our work aims to monitor wheat crop using a variety-based approach by taking into consideration four different phenological stages of wheat crop development. In addition to highlighting the contribution of Red-Edge vegetation indices in mapping wheat dry matter and nitrogen content dynamics, as well as using Random Forest regressor in the estimation of wheat yield, dry matter and nitrogen uptake relying on UAV (Unmanned Aerial Vehicle) multispectral imagery. The study was conducted on an experimental platform with 12 wheat varieties located in Sidi Slimane (Morocco). Several flight missions were conducted using eBee UAV with MultiSpec4C camera according to phenological growth stages of wheat. The proposed methodology is subdivided into two approaches, the first aims to find the most suitable vegetation index for wheat’s biophysical parameters estimation and the second to establish a global model regardless of the varieties to estimate the biophysical parameters of wheat: Dry matter and nitrogen uptake. The two approaches were conducted according to six main steps: (1) UAV flight missions and in-situ data acquisition during four phenological stages of wheat development, (2) Processing of UAV multispectral images which enabled us to elaborate the vegetation indices maps (RTVI, MTVI2, NDVI, NDRE, GNDVI, GNDRE, SR-RE et SR-NIR), (3) Automatic extraction of plots by Object-based image analysis approach and creating a spatial database combining the spectral information and wheat’s biophysical parameters, (4) Monitoring wheat growth by generating dry biomass and wheat’s nitrogen uptake model using exponential, polynomial and linear regression for each variety this step resumes the varietal approach, (5) Engendering a global model employing both linear regression and Random Forest technique, (6) Wheat yield estimation. The proposed method has allowed to predict from 1 up to 21% difference between actual and estimated yield when using both RTVI index and Random Forest technique as well as mapping wheat’s dry biomass and nitrogen uptake along with the nitrogen nutrition index (NNI) and therefore facilitate a careful monitoring of the health and the growth of wheat crop. Nevertheless, some wheat varieties have shown a significant difference in yield between 2.6 and 3.3 t/ha.
Biodegradable Polymer Compounds Reinforced with Banana Fiber for the Production of Protective Bags for Banana Fruits in the Context of Circular Economy
Authors: Bordón Pablo , Paz Rubén , Peñalva Carolina , Vega Gisela , Monzón Mario , García Lidia
Published: January 2021

Bags used to protect and accelerate the ripening of bananas are a clear example of the environmental problem of packaging waste. Small pieces of these non-biodegradable bags are frequently disposed on the soil by accident (environmental conditions and poor handling during the harvest) and remain there for years. This work focuses on the development of protective biodegradable bags reinforced with banana fiber, obtained from waste of the banana plants, thus promoting a circular economy and a more environmentally friendly process. To achieve this, different bio-based composites were tested (processability) by compounding extrusion (biopolymer and banana fiber with different process steps) and blown film extrusion. The bags produced were tested in field and sequentially improved in three generations of biofilms. The results showed that the maximum processable fiber content was 5 wt %. Additionally, the micronizing of the compounds was crucial to simplify the blown film extrusion and improve the smoothness of the bags (scratches avoidance on the banana surface). The final bags (Mater-Bi biopolymer, 5% combed and sieved banana fiber, and 2.5 wt % TiO2 for ultraviolet light filtration), performed better than the conventional ones (faster maturing, i.e., earlier harvest, and easier handling) and fulfilled the biodegradability, composting and ecotoxicity test requirements.
Evaluation of the Production Performance and the Meat Quality of Chickens Reared in Organic System. As Affected by the Inclusion of Calliphora sp. in the Diet
Authors: Sarmiento-García Ainhoa , Palacios Carlos , González-Martín Inmaculada , Revilla Isabel
Published: January 2021

The use of insects can be a possible source of protein. This study uses Calliphora sp. larvae (CLM) as a protein source in 320 one-day-old medium-growing male chicks (RedBro) during their first month of life. Chickens were randomly assigned to four dietary treatments. Each group consisted of 10 animals, and a total of 8 replicas. Control group was fed with a certified organic feed. The experimental treatments were supplemented with 5% (T2), 10% (T3), or 15% (T4) of CLM, reducing in each case the corresponding percentage of feed quantity. Productive development and meat quality were analyzed, and near infrared spectroscopy (NIRS) was used as a tool for classifying the samples. Chickens of T4 showed greater final body weight and total average daily gain, but they reduced consumption and feed conversion ratio (FCR). The chicken breast meat of T4 had lower cooking losses and higher palmitoleic acid content (p < 0.01). NIRS classified correct 92.4% of samples according to the food received. CLM is presented as a potential ingredient for the diet of medium-slow growing chickens raised in organic systems.
Paternal Dietary Methionine Supplementation Improves Carcass Traits and Meat Quality of Chicken Progeny
Authors: Elsharkawy Mohamed Shafey , Chen Ying , Liu Ranran , Tan Xiaodong , Li Wei , El-Wardany Ibrahim , Zhao Dongqin , Zheng Maiqing , Wen Jie , Zhao Guiping
Published: January 2021

The effects that maternal dietary methionine have on progeny have been reported on broilers. However, the paternal effects are not known, so the current study was conducted to explore the influences of paternal dietary methionine (Met) have on progeny carcass traits, meat quality, and related gene expressions. A total of 192 hens and 24 roosters from Ross parent stock at 36 weeks of age were selected. From week 37 to 46, the roosters were allocated to two groups with three replicates of 4 cocks each, (control, 0.28% Met), and methionine group (MET group, 0.28% Met + 0.1% coated Met). The results revealed that, although the heavier live body weight in progeny at day 49 of control group compared to MET group (p < 0.05), the relative eviscerated yield and relative thigh muscle yield were higher in MET group (p < 0.05); but the relative abdominal fat was lower (p < 0.05). In thigh and breast muscles, a positive response of pH24 h value, shear force (g) and drip loss (%) were observed in MET group (p < 0.05). The lightness (L) and redness (a) were increased (p < 0.05) in breast muscles of MET group, while only the redness (a*24 h) and yellowness (b*24 h) were increased (p < 0.05) in thigh muscles of MET group. The gender has a significant (p < 0.05) effect on carcass traits and muscle redness (a*), where these traits improved in males, and no interaction between treatments and gender were observed for these results. The expression levels of PRKAG2 and PRDX4 supported the changes in muscle pH, with these up-regulated in thigh and breast muscles of MET group, the PPP1R3A gene supported the changes in pH value being down-regulated (p < 0.01) in these same muscles. The BCO1 gene expression was consistent with the changes in meat color and was up-regulated (p < 0.01) in thigh muscles of MET group, consistent with the changes in b* color values. Finally, it was concluded that the supplementation of 0.1% Met to rooster diets could improve carcass characteristics and meat quality of progeny.
The Dietary Supplemental Effect of Nitroethanol in Comparison with Monensin on Methane Emission, Growth Performance and Carcass Characteristics in Female Lambs
Authors: Zhang Zhen-Wei , Wang Yan-Lu , Chen Yong-Yan , Zhang Luo-Tong , Zhang Ying-Jie , Liu Yue-Qin , Guo Yun-Xia , Yang Hong-Jian
Published: January 2021

This study was conducted to evaluate the dietary supplemental effects of 2-nitroethanol (NEOH) in comparison with monensin on methane (CH4) emission, growth performance and carcass characteristics in female lambs. Sixty female, small-tailed Chinese Han lambs (3.5 ± 0.3 month) were randomly allotted into three dietary treatment groups: (1) Control group, a basal control diet, (2) monensin group, the basal diet added with 40 mg/kg monensin, (3) NEOH group, the basal diet added with 277 mg/kg nitroethanol, and the feedlotting trial lasted for 70 days. Although dietary addition of monensin and NEOH did not affect nutrient digestibility of lambs, both monensin and NEOH decreased the calculated CH4 production (12.7% vs. 17.4% decrease; p < 0.01). In addition, the CH4 production represents less dietary energy loss in the monensin and NEOH group than in the control, indicating that monensin and NEOH are potent CH4 inhibitors that can reduce dietary energy loss. Dietary addition of monensin and NEOH decreased dry matter intake (p < 0.01); however, they increased the ADG of female lambs (p < 0.01). As a result, both monensin and NEOH increased feed conversion efficiency of the feedlotting lambs (p < 0.01), suggesting that feed energy saved from CH4 production promoted the feed efficiency and ADG in the present study. Except for the fact that NEOH addition increased the net muscle percentage to carcass weight (p = 0.03), neither monensin nor NEOH had a significant influence on carcass characteristics of female lambs (p > 0.05). From an economic point of view, NEOH and monensin caused a reduction in feed consumption costs, therefore resulting in a higher net revenue and economic efficiency than the control. In summary, dietary supplementation of NEOH in comparison with monensin presented a more promoting effect on energy utilization in female lambs by inhibiting rumen methanogenesis more efficiently, and NEOH improved the net revenue and economic efficiency more significantly than monensin.
Melatonin Alleviates Hypoxia-Induced Apoptosis of Granulosa Cells by Reducing ROS and Activating MTNR1B–PKA–Caspase8/9 Pathway
Authors: Tao Jing-Li , Zhang Xuan , Zhou Jia-Qi , Li Cheng-Yu , Yang Ming-Hui , Liu Zhao-Jun , Zhang Liang-Liang , Deng Shou-Long , Zhang Lu , Shen Ming , Liu Guo-Shi , Liu Hong-Lin
Published: January 2021

In mammalian ovaries, the avascular environment within follicular cavity is supposed to cause hypoxic status in granulosa cells (GCs), leading to apoptotic cell death accompanied by cumulative reactive oxygen species (ROS) production. Melatonin (N-acetyl-5-methoxytryptamine, MT), a broad-spectrum antioxidant that exists in porcine follicle fluid, was suggested to maintain GCs survival under stress conditions. In this study, using the established hypoxic model (1% O2) of cultured porcine GCs, we explored the effect of MT on GCs apoptosis. The results showed that MT restored cell viability and reduced the apoptosis of GCs during hypoxia exposure. In addition, GCs treated with MT exhibited decreased ROS levels and increased expression of antioxidant enzymes including heme oxygenase-1 (HO-1), glutathione S-transferase (GST), superoxide dismutase 1 (SOD1), and catalase (CAT) upon hypoxia incubation. Moreover, the hypoxia-induced expression of cleaved caspase 3, 8, and 9 was significantly inhibited after MT treatment. In contrast, blocking melatonin receptor 2 (MTNR1B) with a competitive antagonist 4-phenyl-2-propionamidotetralin (4P-PDOT) diminished the inhibitory effects of MT on caspase 3 activation. By detecting levels of protein kinase (PKA), a downstream kinase of MTNR1B, we further confirmed the involvement of MT–MTNR1B signaling in mediating GCs protection during hypoxia stress. Together, the present data provide mechanistic evidence suggesting the role of MT in defending GCs from hypoxia-induced apoptosis.
Special Issue High-Performance Eco-Efficient Concrete
Authors: Thomas Carlos , de Brito Jorge , Corinaldesi Valeria
Published: January 2021

The benefits of recycling in the construction sector have been widely demonstrated and are unquestionable [...]
Impact of Implant Design and Bone Properties on the Primary Stability of Orthodontic Mini-Implants
Authors: Redžepagić-Vražalica Lejla , Mešić Elmedin , Pervan Nedim , Hadžiabdić Vahidin , Delić Muamer , Glušac Mirza
Published: January 2021

This study investigated the correlation between bone characteristics, the design of orthodontic mini-implants, the pull-out force, and primary stability. This experimental in vitro study has examined commercial orthodontic mini-implants of different sizes and designs, produced by two manufacturers: Tomas-pin SD (Dentaurum, Ispringen, Germany) and Perfect Anchor (Hubit, Seoul, Korea). The total number of 40 mini-implants were tested. There are two properties that are common to all tested implants—one is the material of which they are made (titanium alloy Ti-6Al-4V), and the other is the method of their insertion. The main difference between the mini-implants, which is why they have been selected as the subject of research in the first place, is reflected in their geometry or design. Regardless of the type of implant, the average pull-out forces were found to be higher for a cortical bone thickness (CBTC) of 0.62–0.67 mm on average, compared to the CBTC < 0.62 mm, where the measured force averages were found to be lower. The analysis of variance tested the impact of the mini-implant geometry on the pull-out force and proved that there is a statistically significant impact (p < 0.015) of all three analyzed geometric factors on the pull-out force of the implant. The design of the mini-implant affects its primary stability. The design of the mini-implant affects the pulling force. The bone quality at the implant insertion point is important for primary stability; thus, the increase in the cortical bone thickness increases the value of the pulling force significantly.
Determination of Zenith Angle Dependence of Incoherent Cosmic Ray Muon Flux Using Smartphones of the CREDO Project
Authors: Karbowiak Michał , Wibig Tadeusz , Alvarez Castillo David , Beznosko Dmitriy , Duffy Alan R. , Góra Dariusz , Homola Piotr , Kasztelan Marcin , Niedźwiecki Michał
Published: January 2021

The Cosmic-Ray Extremely Distributed Observatory (CREDO) was established to detect and study ultra high-energy cosmic ray particles. In addition to making use of traditional methods for finding rare and extended cosmic ray events such as professional-grade Extensive Air Shower (EAS) arrays, as well as educational ‘class-room’ detectors, CREDO also makes use of cameras in smartphones as particle detectors. Beyond the primary scientific goal of the CREDO project, to detect Cosmic Ray Ensembles, is the equally important educational goal of the project. To use smartphones for EAS detection, it is necessary to demonstrate that they are capable of effectively registering relativistic charged particles. In this article, we show that the events recorded in the CREDO project database are indeed tracing incoherent cosmic ray muons. The specific observed distribution of zenith angle of charged particle direction corresponds to that expected for muons. It is difficult, if not impossible, to imagine different mechanisms leading to such a distribution, and we believe it clearly demonstrates the suitability of smartphone-based detectors in supporting the more traditional cosmic ray detectors.
A New Approach to Assess the Built Environment Risk under the Conjunct Effect of Critical Slow Onset Disasters: A Case Study in Milan, Italy
Authors: Blanco Cadena Juan Diego , Moretti Nicola , Salvalai Graziano , Quagliarini Enrico , Re Cecconi Fulvio , Poli Tiziana
Published: January 2021

Citizens in dense built environments are susceptible to the simultaneous occurrence of Slow Onset Disaster (SLOD) events, being particularly prone to increasing temperatures and air pollution. Previous research works have assessed these events’ arousal separately and have identified when their intensity is critical. However, few have integrated their analysis, possibly limited by the quality and granularity of available data, the accessibility and distribution of sensors, and measurements not emulating the surroundings of a pedestrian. Thus, this work performed an outdoor meso-scale multi-hazard-based risk analysis to study the aggregated effects of the SLODs mentioned above. The study was carried out to narrow down the time-frames within 2019 in which these two events could have affected citizens’ health the most. A weighted fuzzy logic was applied to superimpose climatic (temperature, humidity, wind speed, and solar irradiance) and air quality (particulate matter, ozone, and ammonium) distress (true risk) on an hourly basis, allocated using set healthy and comfortable ranges for a specific dense urban climate context within Milan (Italy), processing data from Milano via Juvara station. The findings show that sensitive groups were at risk of high temperature and pollution separately during 26% and 29% of summer and mid-season hours, respectively; while multi-hazard risk would arise during 10.93% of summer and mid-season hours, concentrated mainly between 14:00 and 20:00.
In Situ Assembly of Gold Nanoparticles in the Presence of Poly-DADMAC Resulting in Hierarchical and Highly Fractal Nanostructures
Authors: Köhler J. Michael , Kluitmann Jonas
Published: January 2021

The presence of the polycationic macromolecule poly(diallyldimethylammonium chloride) (poly-DADMAC) has a strong effect on the shape and size of colloidal gold nanoparticles formed by the reduction of tetrachloroauric acid with ascorbic acid in aqueous solution. It slows down nanoparticle growth and supports the formation of nonspherical, partially highly fractal and hierarchical nanoparticle shapes. Four structural levels have been recognized from the near-spherical gold nanoparticles in the lower nanometer range over compact aggregates in the midnanometer range and flower and star-like particles in the submicron range up to larger filamentous aggregates. High-contrast scanning electron microscope (SEM) images show that single gold nanoparticles and clusters of them are connected by bundles of macromolecules in large aggregates. The investigation showed that a large spectrum of different nanoparticle shapes and sizes can be accessed by tuning the poly-DADMAC concentrations and their ratio to other reactants. The nanoassemblies with a very high specific surface area might be of interest for SERS and heterogeneous catalysis.
The Importance of Being Versatile: INFN-CHNet MA-XRF Scanner on Furniture at the CCR “La Venaria Reale”
Authors: Sottili Leandro , Guidorzi Laura , Mazzinghi Anna , Ruberto Chiara , Castelli Lisa , Czelusniak Caroline , Giuntini Lorenzo , Massi Mirko , Taccetti Francesco , Nervo Marco , De Blasi Stefania , Torres Rodrigo , Arneodo Francesco , Re Alessandro , Lo Giudice Alessandro
Published: January 2021

At present, the use of non-destructive, non-invasive X-ray-based techniques is well established in heritage science for the analysis and conservation of works of art. X-ray fluorescence (XRF) plays a fundamental role since it provides information on the elemental composition, contributing to the identification of the materials present on the superficial layers of an artwork. Whenever XRF is combined with the capability of scanning an area to provide the elemental distribution on a surface, the technique is referred to as macro X-ray fluorescence (MA-XRF). The heritage science field, in which the technique is extensively applied, presents a large variety of case studies. Typical examples are paintings, ceramics, metallic objects and manuscripts. This work presents an uncommon application of MA-XRF analysis to furniture. Measurements have been carried out with the MA-XRF scanner of the INFN-CHNet collaboration at the Centro di Conservazione e Restauro “La Venaria Reale”, a leading conservation centre in the field. In particular, a chinoiserie lacquered cabinet of the 18th century and a desk by Pietro Piffetti (1701–1777) have been analysed with a focus on the characterisation of decorative layers and different materials (e.g., gilding in the former and ivory in the latter). The measurements have been carried out using a telemeter for non-planar surfaces, and with collimators of 0.8 mm and 0.4 mm diameter, depending on the spatial resolution needed. The combination of the small measuring head with the use of the telemeter and of a small collimator has guaranteed the ability to scan difficult-to-reach areas with high spatial resolution in a reasonable time (20 × 10 mm2 with 0.2 mm step in less than 20 min).
3D Imaging of CRP and Ultrasonic Tomography to Detect Decay in a Living Adult Holm Oak (Quercus ilex L.) in Sardinia (Italy)
Authors: Puxeddu Michele , Cuccuru Francesco , Fais Silvana , Casula Giuseppe , Bianchi Maria Giovanna
Published: January 2021

A field-integrated methodology using 3D ultrasonic tomography supported by close range photogrammetry (CRP) has been developed and evaluated as a tool to detect the presence and patterns of decay forms in a living adult holm oak (Quercus ilex L.) in an urban green area of the city of Cagliari, Sardinia, Italy. Close range photogrammetry was used to compute a high resolution 3D model of the studied tree, texturized with natural colors. Moreover, following the implemented workflow process it was possible to evaluate the deformation pattern of the studied tree over time. In a second step of our integrated approach, and in order to diagnose the state of health of the inner part of the studied tree in a non-invasive way, laboratory and in situ non-invasive ultrasonic techniques were applied. The results of the close range photogrammetry analysis supported the optimal design of the 3D ultrasonic tomography of the living adult holm oak. Ultrasonic tomography is one of the most powerful non-destructive testing techniques for the full-volume inspection of a structure. It produced physical information on the inner structure of the stem of the investigated tree. The results of the study show that the integrated application of close range photogrammetry and 3D ultrasonic tomography is a powerful tool for a highly accurate and objective evaluation of the external and internal decay of trees and for monitoring their conservation states. With the fully integrated approach, the diagnostic process aimed to prevent instability and the failure of trees can be greatly improved.
Inverse Kinematics Data Adaptation to Non-Standard Modular Robotic Arm Consisting of Unique Rotational Modules
Authors: Ondočko Štefan , Svetlík Jozef , Šašala Michal , Bobovský Zdenko , Stejskal Tomáš , Dobránsky Jozef , Demeč Peter , Hrivniak Lukáš
Published: January 2021

The paper describes the original robotic arm designed by our team kinematic design consisting of universal rotational modules (URM). The philosophy of modularity plays quite an important role when it comes to this mechanism since the individual modules will be the building blocks of the entire robotic arm. This is a serial kinematic chain with six degrees of freedom of unlimited rotation. It was modeled in three different environments to obtain the necessary visualizations, data, measurements, structural changes measurements and structural changes. In the environment of the CoppeliaSim Edu, it was constructed mainly to obtain the joints coordinates matching the description of a certain spatial trajectory with an option to test the software potential in future inverse task calculations. In Matlab, the model was constructed to check the mathematical equations in the area of kinematics, the model’s simulations of movements, and to test the numerical calculations of the inverse kinematics. Since the equipment at hand is subject to constant development, its model can also be found in SolidWorks. Thus, the model’s existence in those three environments has enabled us to compare the data and check the models’ structural designs. In Matlab and SolidWorks, we worked with the data imported on joints coordinates, necessitating overcoming certain problems related to calculations of the inverse kinematics. The objective was to compare the results, especially in terms of the position kinematics in Matlab and SolidWorks, provided the initial joint coordinate vector was the same.
Optimization of Photogrammetric Flights with UAVs for the Metric Virtualization of Archaeological Sites. Application to Juliobriga (Cantabria, Spain)
Authors: Luis-Ruiz Julio Manuel de , Sedano-Cibrián Javier , Pereda-García Raúl , Pérez-Álvarez Rubén , Malagón-Picón Beatriz
Published: January 2021

Three-dimensional models are required to virtualize heritage sites. In recent years, different techniques that ease their generation have been consolidated, such as photogrammetry with Unmanned Aerial Vehicles (UAVs). Nonmetric cameras allow relatively inexpensive data collections. Traditional aerial photogrammetry has established methodologies, but there are not commonly used recommendations for the selection of parameters when working with UAV platforms. This research applies the Taguchi Design of Experiments Method, with four parameters (height of flight, forward and lateral overlaps, and inclination angle of the sensor) and three levels (L9 matrix and nine flights), to determine the set that offers the best metric goodness and, therefore, the most faithful model. The Roman civitas of Juliobriga (Cantabria, North of Spain) was selected for this experiment. The optimal flight results of the average signal-to-noise ratio analysis were height of 15 m, forward and lateral overlaps of 80%, and inclination of 0° (nadiral). This research also highlights the noticeable contribution of the inclination in the accuracy of the model with respect to the others, which is 16.4 times higher than that of the less relevant one (height of flight). This leads to propose avoiding inclination angle as a variable, and the sole development of nadiral flights to obtain accurate models.
Sub/Super-Synchronous Oscillation Oriented Dominant Controller Parameters Stability Region Based on Gerschgorin Disk Theorem
Authors: Ke Song , Lin Tao , Sheng Yibiao , Du Hui , Li Shuitian , Chen Baoping , Chen Rusi , Li Qingyan
Published: January 2021

Since the development of renewable power generation, Sub/Super-Synchronous Control Interac-tion (SSCI), has attracted wide attention. Sub/super-synchronous oscillation (SSO) belongs to the category of small signal stability, and its characteristics are closely related to the operation mode and controller parameters in power grid. The operation mode of renewable power system changes frequently, a method to construct controller parameter stability region (PSR) is proposed for online assessment of the matching degree of controller parameters in power grid under current operation mode. Based on Gerschgorin disk theorem (GDT), eigenvalue distribution range of the system state matrix is estimated, thus the feasible value set of the controller parameter is deduced through small signal stability criterion. The stability margin evaluation index is proposed for guiding the prevention and control of SSO. Specifically, the preconditions of the application of the GDT on the PSR construction are discussed, and a construction method of transition matrix is proposed for the PSR construction. Furthermore, to reduce the conservativeness of PSR, an extension method for PSR is given. Finally, the validity of the proposed method is verified by a realistic benchmark. The efficiency of the proposed method is highlighted by comparing with the point-wise eigenvalue calculation of the state matrix.
Laser-Induced Damage Threshold of Nonlinear GaSe and GaSe:In Crystals upon Exposure to Pulsed Radiation at a Wavelength of 2.1 μm
Authors: Zhu Chongqiang , Dyomin Victor , Yudin Nikolay , Antipov Oleg , Verozubova Galina , Eranov Ilya , Zinoviev Mikhail , Podzyvalov Sergey , Zhuravlyova Yelena , Slyunko Yelena , Yang Chunhui
Published: January 2021

The paper defined the laser-induced damage threshold from the fluence and the peak power of GaSe and GaSe:In single crystals upon exposure to nanosecond radiation in the two micron range and assessed the influence of test radiation energy parameters (pulse repetition rate, pulse duration) on the damage threshold. Laser-induced damage threshold was determined with the parameters of the incident radiation close to the pump radiation parameters of promising dual-wavelength optical parametric oscillators (effective pump sources for THz difference frequency oscillators): wavelength was ≈2.1 μm; pulse repetition rates were 10, 12, 14, and 20 kHz; and pulse durations were 15, 18, 20, and 22 ns. The obtained results made it possible to conclude that the value of GaSe damage threshold at a wavelength of 2.091 μm of the incident radiation was influenced by the accumulation effects (the damage threshold decreased as the pulse repetition rate increased). The accumulation effects were more significant in the case of the In-doped sample, since a more significant decrease in the damage threshold was observed with increasing frequency in terms of the peak power and the fluence.
Improving Technological Infrastructure of Distance Education through Trustworthy Platform-Independent Virtual Software Application Pools
Authors: Ergüzen Atilla , Erdal Erdal , Ünver Mahmut , Özcan Ahmet
Published: January 2021

Distance education (DE), which has evolved under the wings of information technologies in the last decade, has become a fundamental part of our modern education system. DE has not only replaced the traditional education method as in social sciences and lifelong learning opportunities but also has significantly strengthened traditional education in mathematics, science, and engineering fields that require practical and intensive study. However, it is deprived of supporting some key elements found in traditional educational approaches such as (i) modern computer laboratories with installed special software suitable for the student’s field of interest; (ii) adequate staff for maintenance and proper functioning of laboratories; (iii) face-to-face technical support; (iv) license fees. For students to overcome these shortcomings, a virtual application pool is needed where they can easily access all the necessary applications via remote access. This research aims to develop a platform-independent virtual laboratory environment for DE students. This article has been developed specifically to guide DE institutions and to make a positive contribution to the literature. Technology Acceptance Model (TAM) has been used to explain student behaviors. It was concluded that students using the platform performed more successful grades (12.89%) on laboratory assessments and that the students using the developed platform were found to be more satisfied with the education process.
Inter-Subject MEG Decoding for Visual Information with Hybrid Gated Recurrent Network
Authors: Li Jingcong , Pan Jiahui , Wang Fei , Yu Zhuliang
Published: January 2021

As an effective brain signal recording technique for neuroscience, magnetoencephalography (MEG) is widely used in cognitive research. However, due to the low signal-to-noise ratio and the structural or functional variabilities of MEG signals between different subjects, conventional methods perform poorly in decoding human brain responds. Inspired by deep recurrent network for processing sequential data, we applied the gated recurrent units for MEG signals processing. In the paper, we proposed a hybrid gated recurrent network (HGRN) for inter-subject visual MEG decoding. Without the need of any information from test subjects, the HGRN effectively distinguished MEG signals evoked by different visual stimulations, face and scrambled face. In the leave-one-out cross-validation experiments on sixteen subjects, our method achieved better performance than many existing methods. For more in-depth analysis, HGRN can be utilized to extract spatial features and temporal features of MEG signals. These features conformed to the previous cognitive studies which demonstrated the practicality of our method for MEG signal processing. Consequently, the proposed model can be considered as a new tool for decoding and analyzing brain MEG signal, which is significant for visual cognitive research in neuroscience.
Auditory Brainstem Responses to Successive Sounds: Effects of Gap Duration and Depth
Authors: Cheng Fan-Yin , Champlin Craig A.
Published: January 2021

Temporal acuity is the ability to differentiate between sounds based on fluctuations in the waveform envelope. The proximity of successive sounds and background noise diminishes the ability to track rapid changes between consecutive sounds. We determined whether a physiological correlate of temporal acuity is also affected by these factors. We recorded the auditory brainstem response (ABR) from human listeners using a harmonic complex (S1) followed by a brief tone burst (S2) with the latter serving as the evoking signal. The duration and depth of the silent gap between S1 and S2 were manipulated, and the peak latency and amplitude of wave V were measured. The latency of the responses decreased significantly as the duration or depth of the gap increased. The amplitude of the responses was not affected by the duration or depth of the gap. These findings suggest that changing the physical parameters of the gap affects the auditory system’s ability to encode successive sounds.
ER Stress-Sensor Proteins and ER-Mitochondrial Crosstalk—Signaling Beyond (ER) Stress Response
Authors: Kumar Vaishali , Maity Shuvadeep
Published: January 2021

Recent studies undoubtedly show the importance of inter organellar connections to maintain cellular homeostasis. In normal physiological conditions or in the presence of cellular and environmental stress, each organelle responds alone or in coordination to maintain cellular function. The Endoplasmic reticulum (ER) and mitochondria are two important organelles with very specialized structural and functional properties. These two organelles are physically connected through very specialized proteins in the region called the mitochondria-associated ER membrane (MAM). The molecular foundation of this relationship is complex and involves not only ion homeostasis through the shuttling of calcium but also many structural and apoptotic proteins. IRE1alpha and PERK are known for their canonical function as an ER stress sensor controlling unfolded protein response during ER stress. The presence of these transmembrane proteins at the MAM indicates its potential involvement in other biological functions beyond ER stress signaling. Many recent studies have now focused on the non-canonical function of these sensors. In this review, we will focus on ER mitochondrial interdependence with special emphasis on the non-canonical role of ER stress sensors beyond ER stress.
CAPE and Neuroprotection: A Review
Authors: Balaha Marwa , De Filippis Barbara , Cataldi Amelia , di Giacomo Viviana
Published: January 2021

Propolis, a product of the honey bee, has been used in traditional medicine for many years. A hydrophobic bioactive polyphenolic ester, caffeic acid phenethyl ester (CAPE), is one of the most extensively investigated active components of propolis. Several studies have indicated that CAPE has a broad spectrum of pharmacological activities as anti-oxidant, anti-inflammatory, anti-viral, anti-fungal, anti-proliferative, and anti-neoplastic properties. This review largely describes CAPE neuroprotective effects in many different conditions and summarizes its molecular mechanisms of action. CAPE was found to have a neuroprotective effect on different neurodegenerative disorders. At the basis of these effects, CAPE has the ability to protect neurons from several underlying causes of various human neurologic diseases, such as oxidative stress, apoptosis dysregulation, and brain inflammation. CAPE can also protect the nervous system from some diseases which negatively affect it, such as diabetes, septic shock, and hepatic encephalopathy, while numerous studies have demonstrated the neuroprotective effects of CAPE against adverse reactions induced by different neurotoxic substances. The potential role of CAPE in protecting the central nervous system (CNS) from secondary injury following various CNS ischemic conditions and CAPE anti-cancer activity in CNS is also reviewed. The structure–activity relationship of CAPE synthetic derivatives is discussed as well.
Functional and Rheological Properties of Vicia faba L. Protein Isolates
Authors: Żmudziński Daniel , Goik Urszula , Ptaszek Paweł
Published: January 2021

A protein isolate (85.5%) was obtained from the Vicia faba L. seeds. The main protein fraction, typical for the seeds of this plant, was found to be most numerous: Legumin (35 kDa) and Vicilin (45 kDa). The hydrodynamic and surface properties of isolate aqueous solutions were studied with the help of dynamic light scattering, ζ-potential, and tensometry in a wide range of concentrations and pH conditions. Selected functional properties, like foaming and emulsifying abilities, were studied. An increase of water solubility was shown with a raising pH, as well as a water holding capacity (WHC). The protein isolate showed a tendency to decrease the surface tension of water solutions, with high hydrophobicity and a negative charge of the isolate enhancing the foaming and emulsifying properties. The analysis of the concentration and the pH influence on selected functional properties indicated alkaline conditions as favorable for good foaming and emulsifying properties of the isolate and affected on their rheological properties.
Effect of Postnatal Nutritional Environment Due to Maternal Diabetes on Beta Cell Mass Programming and Glucose Intolerance Risk in Male and Female Offspring
Authors: Bailbe Danièle , Liu Junjun , Gong Pengfei , Portha Bernard
Published: January 2021

Besides the fetal period, the suckling period is a critical time window in determining long-term metabolic health. We undertook the present study to elucidate the impact of a diabetic suckling environment alone or associated with an in utero diabetic environment on beta cell mass development and the risk of diabetes in the offspring in the long term. To that end, we have compared two experimental settings. In setting 1, we used Wistar (W) rat newborns resulting from W ovocytes (oW) transferred into diabetic GK rat mothers (pGK). These oW/pGK neonates were then suckled by diabetic GK foster mothers (oW/pGK/sGK model) and compared to oW/pW neonates suckled by normal W foster mothers (oW/pW/sW model). In setting 2, normal W rat newborns were suckled by diabetic GK rat foster mothers (nW/sGK model) or normal W foster mothers (nW/sW model). Our data revealed that the extent of metabolic disorders in term of glucose intolerance and beta cell mass are similar between rats which have been exposed to maternal diabetes both pre- and postnatally (oW/pGK/sGK model) and those which have been exposed only during postnatal life (nW/sW model). In other words, being nurtured by diabetic GK mothers from birth to weaning was sufficient to significantly alter the beta cell mass, glucose-induced insulin secretion and glucose homeostasis of offspring. No synergistic deleterious effects of pre-and postnatal exposure was observed in our setting.
The Treatment of Mitral Valve Disease—The Only Thing Constant is Change
Authors: Zacharias Joseph , Greco Ernesto
Published: January 2021

The mitral valve is without doubt the part of the human body that is most under pressure [...]
Acknowledgment to Reviewers of C in 2020
Authors:   C Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that C maintains its standards for the high quality of its published papers [...]
CD123 Is Consistently Expressed on NPM1-Mutated AML Cells
Authors: Perriello Vincenzo Maria , Gionfriddo Ilaria , Rossi Roberta , Milano Francesca , Mezzasoma Federica , Marra Andrea , Spinelli Orietta , Rambaldi Alessandro , Annibali Ombretta , Avvisati Giuseppe , Di Raimondo Francesco , Ascani Stefano , Falini Brunangelo , Martelli Maria Paola , Brunetti Lorenzo
Published: January 2021

NPM1-mutated (NPM1mut) acute myeloid leukemia (AML) comprises about 30% of newly diagnosed AML in adults. Despite notable advances in the treatment of this frequent AML subtype, about 50% of NPM1mut AML patients treated with conventional treatment die due to disease progression. CD123 has been identified as potential target for immunotherapy in AML, and several anti-CD123 therapeutic approaches have been developed for AML resistant to conventional therapies. As this antigen has been previously reported to be expressed by NPM1mut cells, we performed a deep flow cytometry analysis of CD123 expression in a large cohort of NPM1mut and wild-type samples, examining the whole blastic population, as well as CD34+CD38− leukemic cells. We demonstrate that CD123 is highly expressed on NPM1mut cells, with particularly high expression levels showed by CD34+CD38− leukemic cells. Additionally, CD123 expression was further enhanced by FLT3 mutations, which frequently co-occur with NPM1 mutations. Our results identify NPM1-mutated and particularly NPM1/FLT3 double-mutated AML as disease subsets that may benefit from anti-CD123 targeted therapies.
Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated
Authors: O’Connor Rebecca E , Kiazim Lucas G , Rathje Claudia C , Jennings Rebecca L , Griffin Darren K
Published: January 2021

With demand rising, pigs are the world’s leading source of meat protein; however significant economic loss and environmental damage can be incurred if boars used for artificial insemination (AI) are hypoprolific (sub-fertile). Growing evidence suggests that semen analysis is an unreliable tool for diagnosing hypoprolificacy, with litter size and farrowing rate being more applicable. Once such data are available, however, any affected boar will have been in service for some time, with significant financial and environmental losses incurred. Reciprocal translocations (RTs) are the leading cause of porcine hypoprolificacy, reportedly present in 0.47% of AI boars. Traditional standard karyotyping, however, relies on animal specific expertise and does not detect more subtle (cryptic) translocations. Previously, we reported development of a multiple hybridisation fluorescence in situ hybridisation (FISH) strategy; here, we report on its use in 1641 AI boars. A total of 15 different RTs were identified in 69 boars, with four further animals XX/XY chimeric. Therefore, 4.5% had a chromosome abnormality (4.2% with an RT), a 0.88% incidence. Revisiting cases with both karyotype and FISH information, we reanalysed captured images, asking whether the translocation was detectable by karyotyping alone. The results suggest that chromosome translocations in boars may be significantly under-reported, thereby highlighting the need for pre-emptive screening by this method before a boar enters a breeding programme.
Unexpected Pro-Fibrotic Effect of MIF in Non-Alcoholic Steatohepatitis Is Linked to a Shift in NKT Cell Populations
Authors: Heinrichs Daniel , Brandt Elisa F. , Fischer Petra , Köhncke Janine , Wirtz Theresa H. , Guldiken Nurdan , Djudjaj Sonja , Boor Peter , Kroy Daniela , Weiskirchen Ralf , Bucala Richard , Wasmuth Hermann E. , Strnad Pavel , Trautwein Christian , Bernhagen Jürgen , Berres Marie-Luise
Published: January 2021

Macrophage migration inhibitory factor (MIF) is a pleiotropic inflammatory cytokine with anti-fibrotic properties in toxic liver injury models and anti-steatotic functions in non-alcoholic fatty liver disease (NAFLD) attributed to the CD74/AMPK signaling pathway. As NAFLD progression is associated with fibrosis, we studied MIF function during NAFLD-associated liver fibrogenesis in mice and men by molecular, histological and immunological methods in vitro and in vivo. After NASH diet feeding, hepatic Mif expression was strongly induced, an effect which was absent in Mif∆hep mice. In contrast to hepatotoxic fibrosis models, NASH diet-induced fibrogenesis was significantly abrogated in Mif−/− and Mif∆hep mice associated with a reduced accumulation of the pro-fibrotic type-I NKT cell subpopulation. In vitro, MIF skewed the differentiation of NKT cells towards the type-I subtype. In line with the murine results, expression of fibrosis markers strongly correlated with MIF, its receptors, and markers of NKT type-I cells in NASH patients. We conclude that MIF expression is induced during chronic metabolic injury in mice and men with hepatocytes representing the major source. In NAFLD progression, MIF contributes to liver fibrogenesis skewing NKT cell polarization toward a pro-fibrotic phenotype highlighting the complex, context-dependent role of MIF during chronic liver injury.
Exploring the Structural Chemistry of Pyrophosphoramides: N,N′,N″,N‴-Tetraisopropylpyrophosphoramide
Authors: Micallef Duncan , Vella-Zarb Liana , Baisch Ulrich
Published: January 2021

N,N′,N″,N‴-Tetraisopropylpyrophosphoramide 1 is a pyrophosphoramide with documented butyrylcholinesterase inhibition, a property shared with the more widely studied octamethylphosphoramide (Schradan). Unlike Schradan, 1 is a solid at room temperature making it one of a few known pyrophosphoramide solids. The crystal structure of 1 was determined by single-crystal X-ray diffraction and compared with that of other previously described solid pyrophosphoramides. The pyrophosphoramide discussed in this study was synthesised by reacting iso-propyl amine with pyrophosphoryl tetrachloride under anhydrous conditions. A unique supramolecular motif was observed when compared with previously published pyrophosphoramide structures having two different intermolecular hydrogen bonding synthons. Furthermore, the potential of a wider variety of supramolecular structures in which similar pyrophosphoramides can crystallise was recognised. Proton (1H) and Phosphorus 31 (31P) Nuclear Magnetic Resonance (NMR) spectroscopy, infrared (IR) spectroscopy, mass spectrometry (MS) were carried out to complete the analysis of the compound.
Different Countries, Common Support for Climate Change Mitigation: The Case of Germany and Poland
Authors: Bohdanowicz Zbigniew
Published: January 2021

There are numerous studies assessing the influence of individual sociological, political, and demographic factors on attitudes towards climate change. However, there is still a need for a deeper understanding of the reasons behind these attitudes and for research based on results from more than one country. This study empirically examines a range of psychosocial and demographic determinants of support for climate policy (renewable energy, energy efficiency and carbon tax) in Germany and Poland (n = 1969). The results show that the societies of both countries, despite significant differences in income, culture and political stance on climate change, similarly support implementation of climate policies. For both countries valid predictors of support are: awareness, emotional response to climate crisis, sense of control, and belief in effectiveness of solutions; the study also shows predictors relevant in only one country. Factor analysis identified similar dimensions of attitudes toward climate change in both countries. The main findings show that support for climate policy is high in both countries and that the public is ready to accept more ambitious climate goals. Despite the differences between the countries, a coherent climate policy seems justified. The study also shows differences between the countries and provides recommendations for policymakers.
Publisher’s Note: Continued Publication of Clinics and Practice by MDPI
Authors: Lin Shu-Kun , Vicario Unai , Vazquez Franck
Published: January 2021

Clinics and Practice was launched in 2011 and it has been published over the past nine years by PAGEPress Publications [...]