bluuebunny/crossref_metadata_2025 huggingface dataset:
Trace Metals Distribution in Tissues of 10 Different Shark Species from the Eastern Mediterranean Sea
Authors: Roubie Eleni , Karavoltsos Sotirios , Sakellari Aikaterini , Katsikatsos Nikolaos , Dassenakis Manos , Megalofonou Persefoni
Published: February 2024

As long-living apex predators, sharks tend to bioaccumulate trace metals through their diet. The distribution of Al, As, Cd, Co, Cr, Cs, Cu, Fe, Mn, Ni, Pb, V, Zn and Hg in different tissues (muscle, liver, heart, gills and gonads) of large-size (58–390 cm) sharks, some of which rare, of the eastern Mediterranean Sea was studied. Trace metals analyses in samples originating from ten different Chondrichthyes species were performed by inductively coupled plasma–mass spectrometry (ICP-MS) and Cold Vapor Atomic Absorption Spectrometry (CVAAS) for Hg. Data on trace metal levels are for the first time reported herewith for the species O. ferox and H. nakamurai. Higher median concentrations of trace metals were generally determined in the liver. The concentrations of Hg, Cs and As in the muscle increased proportionally with body length. Statistically significant differences between sexes were recorded for Hg, Cr, Ni and As (p = 0.015) in the muscle tissues of P. glauca. Muscle tissue Hg concentrations exceeded the EU maximum limit (1 μg g−1 wet weight) in 67% of the individuals sampled, with the highest concentrations detected in O. ferox and S. zygaena, whereas regarding Pb (limit 0.30 μg g−1 ww), the corresponding percentage was 15%. Arsenic concentrations were also of concern in almost all shark tissues examined.
Influence of Cross Perturbations on Turbulent Kelvin–Helmholtz Instability
Authors: Sementilli Mae , Zangeneh Rozie , Chen James
Published: February 2024

Kelvin–Helmholtz instability has been studied extensively in 2D. This study attempts to address the influence of turbulent flow and cross perturbation on the growth rate of the instability and the development of mixing layers in 3D by means of direct numerical simulation. Two perfect gases are considered to be working fluids moving as opposite streams, inducing shear instability at the interface between the fluids and resulting in Kelvin–Helmholtz instability. The results show that cross perturbation affects the instability by increasing the amplitude growth while adding turbulence has almost no effect on the amplitude growth. Furthermore, by increasing the turbulence intensity, a more distinct presence of the inner flow can be seen in the mixing layer of the two phases, and the presence of turbulence expands the range of high-frequency motion significantly due to turbulence structures. The results give a basis for which 3D Kelvin–Helmholtz phenomena should be further investigated using numerical simulation for predictive modeling, beyond the use of simplified 2D theoretical models.
Evaluation Method of Texture of Glutinous Rice Cakes (Niangao) and Its Key Impact Indicators
Authors: Lyu Qingyun , Wang Xing , Dang Yunzhuo , Zhu Lijie , Chen Lei , Wang Xuedong , Ding Wenping
Published: February 2024

This study aimed to find a unique method to assess the textural properties of Niangao (glutinous rice cakes), to determine the relationship between the textural properties of rice cakes and the indicators of glutinous rice, and to identify the key indicators that significantly affect the textural properties of Niangao. The study encompassed the analysis of the chemical composition and pasting characteristics of 22 glutinous rice varieties, revealing the substantial impact of variety on lipid content, straight-chain starch content, and pasting performance. Subsequently, the textural features of the resulting Niangao were subjected to principal component analysis (PCA) to derive a mathematical method for evaluating their textural attributes, with the obtained scores employed in hierarchical cluster analysis (HCA) to identify 12 key textural characteristics. Further analysis using stepwise linear regression (SLR) demonstrated that the regression model incorporating final and peak viscosities of the glutinous rice significantly predicted the composite score of the Niangao’s textural properties. This highlights the importance of final and peak viscosities as key indicators for assessing the textural quality of Niangao.
Unraveling the Physicochemical Properties and Bacterial Communities in Rabbit Meat during Chilled Storage
Authors: Wu Zhoulin , Xu Maoqin , He Wei , Li Xiaoyu , Qiu Chaoqing , Zhang Jiamin
Published: February 2024

The freshness and bacterial communities of fresh and salted rabbit meat during 8 days of refrigerated storage at 4 °C were evaluated. The results showed that the addition of 2% salt significantly changed the color of meat, of which the lightness (L*), redness (a*), and yellowness (b*) were lower than that of fresh meat over time. The pH of all samples increased during storage, and meat with salt addition had lower values in comparison to fresh samples over time. The total volatile base nitrogen (TVB-N) concentration increased rapidly in salt-treated meat but was significantly (p < 0.05) lower than that in meat without salt added before 6 days. Over time, the content of thiobarbituric acid reactive substances (TBARS) showed a progressive trend, but a rapid increase occurred in salted meat. High-throughput sequencing showed that the microflora of each sample had a positive trend in alpha diversity and a negative trend in beta diversity. Bacterial taxonomic analysis indicated that the initial microbial flora for chilled rabbit meat was dominated by Shigaella, Bacteroides, and Lactococcus, and the population of Brochothrix and Psychrobacter increased over time and became the dominant spoilage bacterium. In particular, the addition of salt significantly reduced the abundance of Psychrobacter and Brochothrix. These findings might provide valuable information regarding the quality monitoring of rabbit meat during chilled storage.
A Culinary and Medicinal Gem: Exploring the Phytochemical and Functional Properties of Thai Basil
Authors: Prasongdee Panita , Posridee Kakanang , Oonsivilai Anant , Oonsivilai Ratchadaporn
Published: February 2024

This study aimed to comprehensively evaluate the potential health benefits of Thai basil extracts from two species, Ocimum basilicum var. thyrsiflorum and Ocimum basilicum cv. Jumbo 4320, by investigating their bioactivities, phytochemical composition, and in vitro antioxidant, antimicrobial, and antithrombotic activities. Thai basil extracts from two species (Ocimum basilicum var. thyrsiflorum and cv. Jumbo 4320) were obtained using water, ethanol, and ethyl acetate. Phytochemical analysis revealed chlorophylls, carotenoids, and diverse phenolic compounds. Its water extract boasted the highest total phenolics (459.62 ± 3.07 mg GAE/100 g), outperforming ethanol and ethyl acetate extracts (171.20 ± 1.10 and 66.02 ± 0.99 mg GAE/100 g, respectively). The ethanol extract of Jumbo 4320 also reigned supreme in total flavonoids (557.12 ± 14.27 mg CE/100 g), surpassing its counterparts (209.07 ± 23.31 and 131.41 ± 0.37 mg CE/100 g). O. basilicum cv. Jumbo 4320 extracts exhibited superior antioxidant activity by various assays. Jumbo 4320′s water extract further dominated in the DPPH assay (IC50 48.52 ± 1.15 mg/mL), demonstrating superior free radical scavenging compared to ethanol and ethyl acetate extracts (IC50 60.54 ± 0.52 and 82.09 ± 3.18 mg/mL), respectively. While thyrsiflorum’s ethanol extract claimed the top spot in the FRAP assay (0.0186 ± 0.00 mmol Fe2+/g), Jumbo 4320′s water extract showcased the highest cellular antioxidant activity (80.62 ± 0.00% relative fluorescence intensity) in the DCFH-DA assay. Interestingly, Ocimum basilicum var. thyrsiflorum extracts demonstrated stronger antithrombotic activity at prolonging the prothrombin time at 78.3 ± 17.56 s. While the antimicrobial activity against most tested pathogens was limited, both species’ extracts inhibited Bacillus cereus. These findings suggest the potential of Thai basil extracts, particularly from Jumbo 4320, as functional food ingredients with antioxidant and antithrombotic properties.
Effect of Beverage Composition on Radio Frequency Identification (RFID) Performance Using Polyethylene Terephthalate (PET) Bottles for Smart Food Packaging Applications
Authors: Claucherty Ethan , Cummins Danielle , Rossi Angelica , Aliakbarian Bahar
Published: February 2024

Radio frequency identification (RFID) technology is crucial in revolutionizing the food supply chain and combating global food waste. However, this technology faces challenges in full integration due to disruptive effects on tags caused by the dielectric properties of food and beverage ingredients, chemical constituents, and their packaging. This paper aims to demonstrate the effect of packaging and beverage contents on RFID tag performance. Three commercially available ultra-high frequency (UHF) RFID tags with different designs were tested on polyethylene terephthalate (PET) bottles, measuring tag performance through sensitivity, backscatter, and read range in the presence of various water-based solutions and commercially available beverages. The results highlight the substantial impact of the beverage type and tag design on RFID performance. The results of this study showed that tag 3 was the most consistent and readable tag amongst those tested in the presence of different beverage contents. Tag 3 resulted in a sensitivity ranging from −0.49 to −2.01 dBm, backscatter from −38.16 to 43.59 dBm, and read range from 1.58 to 1.88 m, while tag 1 performed the best in the presence of an empty PET bottle resulting in a sensitivity of −20.78 dBm, backscatter of −23.65 dBm, and read range of 16.34 m. The results of this study can be used for further investigations to develop a mathematical model that predicts the RFID tag performance based on the food composition. This model will be helpful for the design of the tags while facilitating the adoption of smart packaging for food traceability.
Investigation of the Time Fractional Higher-Dimensional Nonlinear Modified Equation of Wave Propagation
Authors: Liu Jian-Gen , Feng Yi-Ying
Published: February 2024

In this article, we analyzed the time fractional higher-dimensional nonlinear modified model of wave propagation, namely the (3 + 1)-dimensional Benjamin–Bona–Mahony-type equation. The fractional sense was defined by the classical Riemann–Liouville fractional derivative. We derived firstly the existence of symmetry of the time fractional higher-dimensional equation. Next, we constructed the one-dimensional optimal system to the time fractional higher-dimensional nonlinear modified model of wave propagation. Subsequently, it was reduced into the lower-dimensional fractional differential equation. Meanwhile, on the basis of the reduced equation, we obtained its similarity solution. Through a series of analyses of the time fractional high-dimensional model and the results of the above obtained, we can gain a further understanding of its essence.
New Version of Fractional Pachpatte-Type Integral Inequalities via Coordinated ℏ-Convexity via Left and Right Order Relation
Authors: Saeed Tareq , Nwaeze Eze R. , Khan Muhammad Bilal , Hakami Khalil Hadi
Published: February 2024

In particular, the fractional forms of Hermite–Hadamard inequalities for the newly defined class of convex mappings proposed that are known as coordinated left and right ℏ-convexity (LR-ℏ-convexity) over interval-valued codomain. We exploit the use of double Riemann–Liouville fractional integral to derive the major results of the research. We also examine the key results’ numerical validations that examples are nontrivial. By taking the product of two left and right coordinated ℏ-convexity, some new versions of fractional integral inequalities are also obtained. Moreover, some new and classical exceptional cases are also discussed by taking some restrictions on endpoint functions of interval-valued functions that can be seen as applications of these new outcomes.
An Analysis of the Impact of Mining Excavation Velocity on the Development of Gaseous and Gaseous Geodynamic Hazards in Copper Ore Mines
Authors: Gniewosz Maciej , Stopkowicz Agnieszka , Cała Marek
Published: February 2024

The hazards of gaseous geodynamic phenomena and rockbursts are among the most challenging to assess and classify. This perception arises from both a review of the literature and an examination of available instructions and regulations in underground mining facilities. The hazard of gaseous geodynamic phenomena in Polish copper ore mines only appeared in 2009, whereas these phenomena occur and are commonly described in other mining countries. In Polish copper ore mines, due to the room and pillar system in fields with lengths of about 460 m, very often parallel to neighboring fields, which together give a length of about 900 m, it is difficult to identify the location of gas traps due to the large size of the area. This paper presents an analysis of the influence of the velocity of the excavation on the possibility of escalating or reducing the described mining hazards. An analysis of the impact of excavation velocity on the state of gaseous geodynamic and roof fall hazards was conducted for two mining fields. For the considered mining fields, the hypothesis was formulated that an excavation velocity greater than or equal to 17 m/month positively influences a reduction in both gaseous geodynamic and roof fall hazards.
Evaluation of Jacking Forces in Weathered Phyllite Based on In Situ Pressuremeter Testing and Deep Learning
Authors: Yeo Lit Yen , Phangkawira Fredrik , Kueh Pei Gee , Lee Sue Han , Choo Chung Siung , Zhang Dongming , Ong Dominic Ek Leong
Published: February 2024

Pipe jacking is a trenchless technology used to install buried pipelines, such as sewer lines in wastewater management systems. Existing mechanistic approaches based on geomaterial strength parameters (i.e., friction angle and apparent cohesion) can provide an estimation of the potential jacking forces during construction. However, extracting intact rock cores for strength characterisation is challenging when dealing with highly weathered ‘soft rocks’ which exhibit RQD values of zero. Such was the case for a pipe jacking drive traversing the highly weathered lithology underlying Kuching City, Malaysia. Furthermore, mechanistic approaches face limitations during construction when jacking forces are dependent on operation parameters, such as jacking speed and lubrication. To address these knowledge gaps, the primary objectives of this study are the development of rock strength parameters based on in situ pressuremeter testing for the purpose of estimating jacking forces. Furthermore, this study investigates the influence of various pipe jacking operation parameters, with a particular focus on their impact on jacking forces in weathered ‘soft rocks’. To achieve this, a novel deep learning model with an attention mechanism is introduced. The proposed methods of rock strength parameters derived from pressuremeter testing and the utilisation of deep learning will help to provide insights into the key factors affecting the development of jacking forces. This paper successfully shows the use of in situ pressuremeter testing in developing Mohr–Coulomb (MC) parameters directly from the site. In addition, the developed deep learning model with an attention mechanism successfully highlights the significance of pipe jacking operation parameters with an accuracy of 88% in predicting the jacking forces.
Growing Rod versus Posterior Spinal Fusion Treatment of Juvenile Idiopathic Scoliosis: Unique Characteristics and Surgical Outcomes
Authors: Wacker Elizabeth M. , Schultz Lindsay , Leitsinger Nichole , Jain Viral V. , Sturm Peter F.
Published: February 2024

Progressive spinal curvature in juvenile idiopathic scoliosis (JIS) is challenging to treat. When conservative management fails, treatments include growing rods (GRs) or posterior spinal fusion (PSF). The purpose of this study is to compare the patient characteristics and outcomes of GR and PSF treatment of JIS. We performed a retrospective review of demographic, radiographic, and surgical data for all JIS patients requiring surgical treatment between 2012 and 2020. Patients who underwent any GR treatment were compared to PSF patients. A total of 36 patients (13 GR, 23 PSF) were reviewed. PSF patients had a larger pre-operative spinal height (p = 0.002), but similar pre-operative major curve magnitudes (p = 0.558). PSF treatment resulted in similar change in the T1-S1 length (p = 0.002), but a greater correction of the curve magnitude (p < 0.055) compared to GR patients. Eight patients initially treated with GRs later underwent definitive PSF treatment. This subset of patients had a greater spinal height before PSF (p = 0.006), but similar immediate post-PSF T1-S1 lengths (p = 0.437) and smaller changes in spinal height from PSF (p = 0.020) than primary PSF patients. At final follow-up, patients who underwent primary PSF versus PSF after GR had similar spinal heights (p = 0.842). The surgical intervention chosen to manage progressive JIS often differs based on patient characteristics. While this choice may impact immediate outcomes, the outcomes at final follow up are similar.
Identifying and Evaluating Young Children with Developmental Central Hypotonia: An Overview of Systematic Reviews and Tools
Authors: Hidalgo Robles Álvaro , Paleg Ginny S. , Livingstone Roslyn W.
Published: February 2024

Children with developmental central hypotonia have reduced muscle tone secondary to non-progressive damage to the brain or brainstem. Children may have transient delays, mild or global functional impairments, and the lack of a clear understanding of this diagnosis makes evaluating appropriate interventions challenging. This overview aimed to systematically describe the best available evidence for tools to identify and evaluate children with developmental central hypotonia aged 2 months to 6 years. A systematic review of systematic reviews or syntheses was conducted with electronic searches in PubMed, Medline, CINAHL, Scopus, Cochrane Database of Systematic Reviews, Google Scholar, and PEDro and supplemented with hand-searching. Methodological quality and risk-of-bias were evaluated, and included reviews and tools were compared and contrasted. Three systematic reviews, an evidence-based clinical assessment algorithm, three measurement protocols, and two additional measurement tools were identified. For children aged 2 months to 2 years, the Hammersmith Infant Neurological Examination has the strongest measurement properties and contains a subset of items that may be useful for quantifying the severity of hypotonia. For children aged 2–6 years, a clinical algorithm and individual tools provide guidance. Further research is required to develop and validate all evaluative tools for children with developmental central hypotonia.
Hospital Care for Cancer Patients—Education and Respect for Patient Rights
Authors: Borowska Mariola , Religioni Urszula , Mańczuk Marta
Published: February 2024

This study aims to examine cancer patients’ opinions of safety, the observance of patient’s rights, and the quality of healthcare. Such an analysis will allow for the identification of areas for improvement in quality, safety, and communication between medical staff and patients. Cancer patients are a special kind of patients with chronic and complex diseases, so we need to observe the type of communication they use, which is a critical issue in a hospital ward but also has a significant impact on how the patient follows recommendations at home. Observing a patient’s rights impacts the safety and quality of medical care. This information allows for the identification of areas requiring deeper analysis and improvement. This study was based on a survey conducted at an oncology hospital. The survey contained questions divided into seven sections related to the study areas. Our study emphasizes the importance of knowledge and understanding regarding patient rights among medical staff and patients, underscoring their role in ensuring quality and safety in healthcare settings. We found a strong correlation between the politeness of medical receptionists and staff and patient perceptions of the clarity and exhaustiveness of the information provided.
Supplement Consumption by Elite Soccer Players: Differences by Competitive Level, Playing Position, and Sex
Authors: Sebastiá-Rico Jaime , Martínez-Sanz José Miguel , Sanchis-Chordà Jesús , Alonso-Calvar Miguel , López-Mateu Pedro , Romero-García David , Soriano Jose M.
Published: February 2024

Soccer is a sport practiced all over the world and whose practice begins in young athletes. Currently, the consumption of nutritional supplements is essential to achieve the maximum performance of players. The aim of this study was to describe the consumption of sports supplements (CSS) by elite soccer players and its association with their competitive level, playing position, and sex. A comparative descriptive and non-experimental study was performed during the 2021–2022 competitive season. A total of 70 elite players completed one online questionnaire about their CSS. We found that sports drinks (55.7%), sports bars (50.0%), whey protein (48.6%), caffeine (47.1%), and creatine (60.0%) were the most consumed supplements by the total sample. Relative to the categories, the CSS was higher in the senior teams for both men and women. Regarding playing positions, caffeine was more consumed by midfielders and forwards (p = 0.013). Finally, in relation to sex, significant differences were found in the consumption of sports confectionery (p = 0.036), whey protein (p = 0.002), β-alanine (p = 0.013), and melatonin (p = 0.016). Soccer club SS questionnaires gather data to understand patterns, assess effectiveness and risks, and aid research. In conclusion, differences were found in the CSS according to competitive level, sex, and playing position.
Ageing with Parkinson’s: Identification of Personal Needs in the Northern Spanish Context
Authors: García Patricia Solís , Souto Serrano María , Alcedo Rodríguez Mª Ángeles , Peña Suárez Elsa , Pedrosa Ignacio , Diaz Antonio León Aguado
Published: February 2024

As individuals diagnosed with Parkinson’s disease enter older age, the myriad challenges and complications associated with this condition tend to escalate. Hence, there is a critical necessity to comprehensively discern the perceived needs of these individuals, along with their proposed remedies and essential support requisites. Additionally, understanding the perspectives of their families becomes imperative to formulate tailored interventions aimed at enhancing their overall development, progression, and quality of life. The study’s main objective is to assess the perceived needs of individuals with PD and their family members, propose necessary solutions, and suggest future perspectives. The study encompassed a cohort of 268 participants, comprising 179 individuals diagnosed with Parkinson’s disease and 89 of their relatives. A meticulously designed structured interview instrument consisting of 93 items was employed to assess various domains encompassing perceived needs, institutional support mechanisms, essential solutions, and future anticipations. Results: Statistically significant differences were found in health resources, social services resources, obstacles, solutions, and future outlook, with higher mean values from the relatives. Conclusions: The results highlight the most concerning needs in this context. Specifically, those needs related to health resources, social services resources, and future outlook present the greatest differences between the two subsamples, with the family members perceiving more needs. This alignment extended to both the categorization of unmet needs and the requisite solutions envisioned to address them. Suggested improvements include a sociosanitary strategy, stakeholder involvement, and prioritizing flexible home assistance to support older individuals with PD and their families.
Digitalization of Activities of Daily Living and Its Influence on Social Participation for Community-Dwelling Older Adults: A Scoping Review
Authors: Mendoza-Holgado Cristina , García-González Inmaculada , López-Espuela Fidel
Published: February 2024

Everyday technology (ET) has been defined as the broad set of devices and artefacts that are currently present in people’s lives both inside and outside of the home. A subgroup within ET is known as everyday information and communication technologies (EICTs). The main characteristic of these technologies is that they can facilitate or disrupt the engagement of individuals in their daily activities. This scoping review aims to identify what is known about how ET can function as facilitators or barriers to occupations, such as the social participation of older adults. The proposed scoping review was conducted in accordance with the Joanna Briggs Institute (JBI) methodology for scoping reviews. The review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Review (PRISMA-ScR) guidelines and checklist. The scoping review was conducted across five online databases (PubMed, Scopus, Web of Science, MEDLINE and PsycoINFO [EBSCO]) to identify published, peer-reviewed records. Studies were screened by two independent reviewers against the inclusion criteria. This review considered studies concerning the use of ET or EICTs in community-dwelling adults aged over 60 years, with or without cognitive impairment or dementia. All of the articles were in English, and reviews were not included. Eleven papers were selected and showed that despite the increasing demand for technologies of daily life and the digitalization of society and processes, according to our research, few studies addressed the limitations in the social participation of older adults. In conclusion, ET can provide a way to promote and maintain the personal autonomy for older adults in community dwellings. However, cognitive impairment hinders the use of electronic technologies and increases perceived problems.
The Autism Spectrum Disorder and Its Possible Origins in Pregnancy
Authors: Botelho Rayane M. , Silva Ana Lúcia M. , Borbely Alexandre U.
Published: February 2024

Autism Spectrum Disorder (ASD) belongs to the group of neurodevelopmental disorders, and has a high prevalence, affecting 1 in 100 children according to data from the World Health Organization (WHO). To be diagnosed with ASD, the child must have persistent deficits in communication and social interactions, and restricted and repetitive patterns of behavior, interests, or activities. Despite its prevalence, the etiology of ASD is still uncertain, with multifactorial characteristics, including those associated with the gestational period, where maternal exposure to biological, chemical, or physical hazards occurs, some of which have already been proposed as causes of ASD outcomes. Since pregnancy requires a balance between the maternal–fetal binomial, the breakdown of this balance caused by such environmental hazards can lead to altered fetal neurodevelopment, including ASD. With this firmly in mind, this review aims to compile the most recent data on the gestational causes that may be associated with the development of ASD to help health professionals identify risk factors and act for the prevention and management of ASD.
Learning Universal Trajectory Representation via a Siamese Geography-Aware Transformer
Authors: Wu Chenhao , Xiang Longgang , Chen Libiao , Zhong Qingcen , Wu Xiongwei
Published: February 2024

With the development of location-based services and data collection equipment, the volume of trajectory data has been growing at a phenomenal rate. Raw trajectory data come in the form of sequences of “coordinate-time-attribute” triplets, which require complicated manual processing before they can be used in data mining algorithms. Current works have started to explore the emerging deep representation learning method, which maps trajectory sequences to vector space and applies them to various downstream applications for boosting accuracy and efficiency. In this work, we propose a universal trajectory representation learning method based on a Siamese geography-aware transformer (TRT for short). Specifically, we first propose a geography-aware encoder to model geographical information of trajectory points. Then, we apply a transformer encoder to embed trajectory sequences and use a Siamese network to facilitate representation learning. Furthermore, a joint training strategy is designed for TRT. One of the training objectives is to predict the masked trajectory point, which makes the trajectory representation robust to low sampling rates and noises. The other is to distinguish the difference between trajectories by means of contrastive learning, which makes the trajectory representation more uniformly distributed over the hypersphere. Last, we design a benchmark containing four typical traffic-related tasks to evaluate the performance of TRT. Comprehensive experiments demonstrate that TRT consistently outperforms the state-of-the-art baselines across all tasks.
The Nuclear Localization Signal of Porcine Circovirus Type 4 Affects the Subcellular Localization of the Virus Capsid and the Production of Virus-like Particles
Authors: Zheng Jiawei , Li Nan , Li Xue , Han Yaqi , Lv Xinru , Zhang Huimin , Ren Linzhu
Published: February 2024

Porcine circovirus 4 (PCV4) is a newly identified virus belonging to PCV of the Circoviridae family, the Circovirus genus. We previously found that PCV4 is pathogenic in vitro, while the virus’s replication in cells is still unknown. In this study, we evaluated the N-terminal of the PCV4 capsid (Cap) and identified an NLS at amino acid residues 4–37 of the N-terminus of the PCV4 Cap, 4RSRYSRRRRNRRNQRRRGLWPRASRRRYRWRRKN37. The NLS was further divided into two fragments (NLS-A and NLS-B) based on the predicted structure, including two α-helixes, which were located at 4RSRYSRRRRNRRNQRR19 and 24PRASRRRYRWRRK36, respectively. Further studies showed that the NLS, especially the first α-helixes formed by the NLS-A fragment, determined the nuclear localization of the Cap protein, and the amino acid 4RSRY7 in the NLS of the PCV4 Cap was the critical motif affecting the VLP packaging. These results will provide a theoretical basis for elucidating the infection mechanism of PCV4 and developing subunit vaccines based on VLPs.
The Impacts of Periconceptional Alcohol on Neonatal Ovaries and Subsequent Adult Fertility in the Rat
Authors: Steane Sarah E. , Burgess Danielle J. , Moritz Karen M. , Akison Lisa K.
Published: February 2024

Maternal exposures during pregnancy can impact the establishment of the ovarian reserve in offspring, the lifetime supply of germ cells that determine a woman’s reproductive lifespan. However, despite alcohol consumption being common in women of reproductive age, the impact of prenatal alcohol on ovarian development is rarely investigated. This study used an established rat model of periconceptional ethanol exposure (PCEtOH; 12.5% v/v ethanol) for 4 days prior to 4 days post-conception. Ovaries were collected from neonates (day 3 and day 10), and genes with protein products involved in regulating the ovarian reserve analyzed by qPCR. Adult offspring had estrous cycles monitored and breeding performance assessed. PCEtOH resulted in subtle changes in expression of genes regulating apoptosis at postnatal day (PN) 3, whilst those involved in regulating growth and recruitment of primordial follicles were dysregulated at PN10 in neonatal ovaries. Despite these gene expression changes, there were no significant impacts on breeding performance in adulthood, nor on F2-generation growth or survival. This contributes additional evidence to suggest that a moderate level of alcohol consumption exclusively around conception, when a woman is often unaware of her pregnancy, does not substantially impact the fertility of her female offspring.
Pulsed Electromagnetic Fields (PEMFs) Trigger Cell Death and Senescence in Cancer Cells
Authors: Pantelis Pavlos , Theocharous Giorgos , Veroutis Dimitris , Vagena Ioanna-Aglaia , Polyzou Aikaterini , Thanos Dimitris-Foivos , Kyrodimos Efthymios , Kotsinas Athanassios , Evangelou Konstantinos , Lagopati Nefeli , Gorgoulis Vassilis G. , Kotopoulos Nicholas
Published: February 2024

The currently available anti-cancer therapies, such as gamma-radiation and chemotherapeutic agents, induce cell death and cellular senescence not only in cancer cells but also in the adjacent normal tissue. New anti-tumor approaches focus on limiting the side effects on normal cells. In this frame, the potential anti-tumor properties of Pulsed Electromagnetic Fields (PEMFs) through the irradiation of breast cancer epithelial cells (MCF-7 and MDA-MB-231) and normal fibroblasts (FF95) were investigated. PEMFs had a frequency of 8 Hz, full-square wave type and magnetic flux density of 0.011 T and were applied twice daily for 5 days. The data collected showcase that PEMF application decreases the proliferation rate and viability of breast cancer cells while having the opposite effect on normal fibroblasts. Moreover, PEMF irradiation induces cell death and cellular senescence only in breast cancer cells without any effect in the non-cancerous cells. These findings suggest PEMF irradiation as a novel, non-invasive anti-cancer strategy that, when combined with senolytic drugs, may eliminate both cancer and the remaining senescent cells, while simultaneously avoiding the side effects of the current treatments.
Biological Activity and Chemical Composition of Propolis Extracts with Potential Use in Vulvovaginal Candidiasis Management
Authors: Silva Ana Margarida , Rocha Beatriz , Moreira Manuela M. , Delerue-Matos Cristina , das Neves José , Rodrigues Francisca
Published: February 2024

Environmental sustainability is an increasing challenge in the pharmaceutical field, leading to the search for eco-friendly active ingredients. Among natural ingredients, propolis arises as an excellent alternative, being a complex substance with pharmacological properties. This work aims to explore the potential of propolis as a new pharmaceutical ingredient for the replacement of conventional vulvovaginal antifungals. Propolis extracts were obtained by Ultrasound-Assisted Extraction using different solvents (water, water/ethanol (50:50, v/v), and ethanol). Afterwards, the extracts were characterized regarding total phenolic content (TPC), antioxidant/antiradical activities, radical scavenging capacity, antifungal activity against strains of Candida species, and viability effect on two female genital cell lines. The aqueous extract achieved the best TPC result as well as the highest antioxidant/antiradical activities and ability to capture reactive oxygen species. A total of 38 phenolic compounds were identified and quantified by HPLC, among which ferulic acid, phloridzin and myricetin predominated. Regarding the anti-Candida spp. activity, the aqueous and the hydroalcoholic extracts achieved the best outcomes (with MIC values ranging between 128 and 512 μg/mL). The cell viability assays confirmed that the aqueous extract presented mild selectivity, while the hydroalcoholic and alcoholic extracts showed higher toxicities. These results attest that propolis has a deep potential for vulvovaginal candidiasis management, supporting its economic valorization.
Stabilization of G-Quadruplex Structures of the SARS-CoV-2 Genome by TMPyP4, BRACO19, and PhenDC3
Authors: Cervenak Miklós , Molnár Orsolya Réka , Horváth Péter , Smeller László
Published: February 2024

The G-quadruplex is one of the non-canonical structures formed by nucleic acids, which can be formed by guanine-rich sequences. They became the focus of much research when they were found in several oncogene promoter regions and also in the telomeres. Later on, they were discovered in viruses as well. Various ligands have been developed in order to stabilize DNA G-quadruplexes, which were believed to have an anti-cancer or antiviral effect. We investigated three of these ligands, and whether they can also affect the stability of the G-quadruplex-forming sequences of the RNA genome of SARS-CoV-2. All three investigated oligonucleotides showed the G-quadruplex form. We characterized their stability and measured their thermodynamic parameters using the Förster resonance energy transfer method. The addition of the ligands caused an increase in the unfolding temperature, but this effect was smaller compared to that found earlier in the case of G-quadruplexes of the hepatitis B virus, which has a DNA genome.
Using the C14:1/Medium-Chain Acylcarnitine Ratio Instead of C14:1 to Reduce False-Positive Results for Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency in Newborn Screening in Japan
Authors: Tajima Go , Aisaki Junko , Hara Keiichi , Tsumura Miyuki , Kagawa Reiko , Sakura Fumiaki , Sasai Hideo , Yuasa Miori , Shigematsu Yosuke , Okada Satoshi
Published: February 2024

Very-long-chain acyl-CoA dehydrogenase (VLCAD) deficiency is a long-chain fatty acid oxidation disorder that manifests as either a severe phenotype associated with cardiomyopathy, a hypoglycemic phenotype, or a myopathic phenotype. As the hypoglycemic phenotype can cause sudden infant death, VLCAD deficiency is included in newborn screening (NBS) panels in many countries. The tetradecenoylcarnitine (C14:1) level in dried blood specimens is commonly used as a primary marker for VLCAD deficiency in NBS panels. Its ratio to acetylcarnitine (C2) and various other acylcarnitines is used as secondary markers. In Japan, tandem mass spectrometry-based NBS, initially launched as a pilot study in 1997, was introduced to the nationwide NBS program in 2013. In the present study, we evaluated levels of acylcarnitine with various chain lengths (C18 to C2), free carnitine, and their ratios in 175 infants who tested positive for VLCAD deficiency with C14:1 and C14:1/C2 ratios. Our analyses indicated that the ratios of C14:1 to medium-chain acylcarnitines (C10, C8, and C6) were the most effective markers in reducing false-positive rates. Their use with appropriate cutoffs is expected to improve NBS performance for VLCAD deficiency.
History of Neonatal Screening of Congenital Hypothyroidism in Portugal
Authors: Costeira Maria José , Costa Patrício , Roque Susana , Carvalho Ivone , Vilarinho Laura , Palha Joana Almeida
Published: February 2024

Congenital hypothyroidism (CH) leads to growth and development delays and is preventable with early treatment. Neonatal screening for CH was initiated in Portugal in 1981. This study examines the history of CH screening in the country. Data were obtained from annual reports and from the national database of neonatal screening laboratory. The CH screening strategy primarily relies on the thyroid-stimulating hormone (TSH), followed by total thyroxine measurement as the second tier for confirmation. The TSH cutoff started at 90 mIU/L, decreasing to the actual 10 mIU/L. The coverage of the screening program has increased rapidly; although voluntary, it reached about 90% in 6 years and became universal in 10 years. Guideline and cutoff updates led to the identification of over 200 additional cases, resulting in specific retesting protocols for preterm and very-low-birth-weight babies. The actual decision tree considers CH when TSH levels are above 40 mIU/L. Data from the CH screening also provide an indication of the iodine status of the population, which is presently indicative of iodine insufficiency. The Portuguese neonatal screening for CH is a history of success. It has rapidly and continuously adapted to changes in knowledge and has become a universal voluntary practice within a few years.
Topic Extraction: BERTopic’s Insight into the 117th Congress’s Twitterverse
Authors: Mendonça Margarida , Figueira Álvaro
Published: February 2024

As social media (SM) becomes increasingly prevalent, its impact on society is expected to grow accordingly. While SM has brought positive transformations, it has also amplified pre-existing issues such as misinformation, echo chambers, manipulation, and propaganda. A thorough comprehension of this impact, aided by state-of-the-art analytical tools and by an awareness of societal biases and complexities, enables us to anticipate and mitigate the potential negative effects. One such tool is BERTopic, a novel deep-learning algorithm developed for Topic Mining, which has been shown to offer significant advantages over traditional methods like Latent Dirichlet Allocation (LDA), particularly in terms of its high modularity, which allows for extensive personalization at each stage of the topic modeling process. In this study, we hypothesize that BERTopic, when optimized for Twitter data, can provide a more coherent and stable topic modeling. We began by conducting a review of the literature on topic-mining approaches for short-text data. Using this knowledge, we explored the potential for optimizing BERTopic and analyzed its effectiveness. Our focus was on Twitter data spanning the two years of the 117th US Congress. We evaluated BERTopic’s performance using coherence, perplexity, diversity, and stability scores, finding significant improvements over traditional methods and the default parameters for this tool. We discovered that improvements are possible in BERTopic’s coherence and stability. We also identified the major topics of this Congress, which include abortion, student debt, and Judge Ketanji Brown Jackson. Additionally, we describe a simple application we developed for a better visualization of Congress topics.
CT and MR Appearance of Teeth: Analysis of Anatomy and Embryology and Implications for Disease
Authors: Abramson Zachary , Oh Chris , Wells Martha , Choudhri Asim F. , Whitehead Matthew T.
Published: February 2024

Abnormalities of dental development and anatomy may suggest the presence of congenital or acquired anomalies. The detection of abnormalities, therefore, is an important skill for radiologists to achieve. Knowledge of dental embryology and an understanding of the radiologic appearances of teeth at various stages of maturation are required for the appreciation of abnormal dental development. While many tooth abnormalities are well-depicted on dedicated dental radiographs, the first encounter with a dental anomaly may be by a radiologist on a computed tomographic (CT) or magnetic resonance (MR) exam performed for other reasons. This article depicts normal dental anatomy and development, describing the appearance of the neonatal dentition on CT and MRI, the modalities most often encountered by clinical radiologists. The radiology and dental literature are reviewed, and key concepts are illustrated with supplemental cases from our institution. The value of knowledge of dental development is investigated using the analysis of consecutive MR brain examinations. Finally, the anatomical principles are applied to the diagnosis of odontogenic infection on CT. Through analysis of the literature and case data, the contrast of dental pathology with normal anatomy and development facilitates the detection and characterization of both congenital and acquired dental disease.
Efficacy of Alveolar Ridge Preservation in Periodontally Compromised Molar Extraction Sites: A Systematic Review and Meta-Analysis
Authors: Fok Melissa Rachel , Pelekos George , Jin Lijian
Published: February 2024

Aim: To investigate the efficacy of alveolar ridge preservation (ARP) in periodontally compromised molar extraction sites. Methods: An electronic search was performed on 10th November 2023 across five databases, seeking randomised/non-randomised controlled trials (RCTs/NCTs) that included a minimum follow-up duration of four months. The RoB2 and Robins-I tools assessed the risk of bias for the included studies. Data on alveolar ridge dimensional and volumetric changes, keratinized mucosal width, and need for additional bone augmentation for implant placement were collected. Subsequently, a meta-analysis was carried out to derive the pooled estimates. Results: Six studies were incorporated in the present review, and a total of 135 molar extraction sockets in 130 subjects were included in the meta-analysis. ARP was undertaken in 68 sites, and 67 sites healed spontaneously. The follow-up time ranged from 4 to 6 months. The meta-analysis of both RCTs and NCTs showed significant differences in mid-buccal ridge width changes at 1 mm level below ridge crest with a mean difference (MD) of 3.80 (95% CI: 1.67–5.94), mid-buccal ridge height changes (MD: 2.18; 95% CI: 1.25–3.12) and volumetric changes (MD: 263.59; 95% CI: 138.44–388.74) in favour of ARP, while the certainty of evidence is graded low to very low. Moreover, ARP appeared to reduce the need for additional sinus and bone augmentation procedures at implant placement with low certainty of evidence. Conclusions: Within the limitations of this study, alveolar ridge preservation in periodontally compromised extraction sites may, to some extent, preserve the ridge vertically and horizontally with reference to spontaneous healing. However, it could not eliminate the need for additional augmentation for implant placement. Further, longitudinal studies with large sample sizes and refined protocols are needed.
Dynamic Positioning Control for Marine Crafts: A Survey and Recent Advances
Authors: Gao Xiaoyang , Li Tieshan
Published: February 2024

This paper surveys the recent advances in dynamic positioning (DP) control for marine crafts. DP of marine crafts means that a craft can maintain a fixed position and heading, or move along a predetermined trajectory slowly without the anchoring system, using only its own thruster system to counteract ocean disturbances. The survey is by no means exhaustive but provides a survey of some of the major technological advancements in DP controller design over the years of research and development. Firstly, the model of marine crafts and some difficult problems in DP control are introduced including the impact of multiple source disturbance, unavailable velocity measurement information, resource conservation and performance optimization, destabilizing impact of faults and network security and compound multi-constraint restrictions. Then, the DP control schemes in recent years are summarized and classified in detail. Finally, some theoretical and technical problems are proposed, including online data-driven model-free control, man–machine combination intelligent control and composite hierarchical anti-disturbance control to guide future investigations.
Green Manuring and Irrigation Strategies Positively Influence the Soil Characteristics and Yield of Coriander (Coriandrum sativum L.) Crop under Salinity Stress
Authors: Sánchez-Navarro Antonio , Girona-Ruíz Aldara , Delgado-Iniesta María José
Published: February 2024

This study shows the influence of soil salinity and irrigation dose on biomass production and its impact on some edaphic indicators and functions. For this purpose, an experiment was carried out in two representative soils from Murcia (SE Spain), one slightly saline (LS) and the other saline (S), where an oat–vetch green manure was intercalated between a spinach cycle and a coriander cycle; the latter being subjected to three different irrigation doses (deficient, optimum and surplus). Rapid response indicators (ECext, cations and anions in the soil solution, etc.) were monitored, as well as the material balances, in particular C and salts. Green manure and crop residues increased soil OC by 12.5% and reduced Na+ and NO3− concentrations. Total biomass production was also affected by salinity, both in oat–vetch, 35.9 and 31. 9 tm ha−1 in LS and S, respectively, and in the coriander crop, where the irrigation dose was decisive, obtaining around 29 tm ha−1 with the optimum and surplus doses and significantly lower amounts with the deficit dose: 20.4 tm ha−1 in LSD and 14. 0 in SD. Therefore, it is necessary to adjust the irrigation doses, since deficit irrigation significantly reduces production and the surplus does not lead to an increase with respect to the optimum, while also causing ions to leach to depth horizons, as is the case for NO3−, Cl− and Na+, with the consequent risk of contaminating the water table.