bluuebunny/crossref_metadata_2025 huggingface dataset:
Shaping Modern Vaccines: Adjuvant Systems Using MicroCrystalline Tyrosine (MCT®)
Authors: Heath Matthew D. , Mohsen Mona O. , de Kam Pieter-Jan , Carreno Velazquez Thalia L. , Hewings Simon J. , Kramer Matthias F. , Kündig Thomas M. , Bachmann Martin F. , Skinner Murray A.
Published: November 2020

The concept of adjuvants or adjuvant systems, used in vaccines, exploit evolutionary relationships associated with how the immune system may initially respond to a foreign antigen or pathogen, thus mimicking natural exposure. This is particularly relevant during the non-specific innate stage of the immune response; as such, the quality of this response may dictate specific adaptive responses and conferred memory/protection to that specific antigen or pathogen. Therefore, adjuvants may optimise this response in the most appropriate way for a specific disease. The most commonly used traditional adjuvants are aluminium salts; however, a biodegradable adjuvant, MCT®, was developed for application in the niche area of allergy immunotherapy (AIT), also in combination with a TLR-4 adjuvant—Monophosphoryl Lipid A (MPL®)—producing the first adjuvant system approach for AIT in the clinic. In the last decade, the use and effectiveness of MCT® across a variety of disease models in the preclinical setting highlight it as a promising platform for adjuvant systems, to help overcome the challenges of modern vaccines. A consequence of bringing together, for the first time, a unified view of MCT® mode-of-action from multiple experiments and adjuvant systems will help facilitate future rational design of vaccines while shaping their success.
Genetic Susceptibility to Antisynthetase Syndrome Associated With Single-Nucleotide Variants in the IL1B Gene That Lead Variation in IL-1β Serum Levels
Authors: Ponce-Gallegos Marco Antonio , Ramos-Martínez Espiridión , García-Carmona Adriana , Mejía Mayra , Nava-Quiroz Karol J. , Pérez-Rubio Gloria , Ambrocio-Ortiz Enrique , González-Pérez Montserrat I. , Buendía-Roldán Ivette , Rojas-Serrano Jorge , Falfán-Valencia Ramcés
Published: November 2020

The antisynthetase syndrome (ASSD) is an autoimmune disorder characterized by myositis, arthritis, mechanic's hands, fever, Raynaud phenomenon, and interstitial lung disease (ILD). We aimed to evaluate single-nucleotide polymorphisms in the interleukin 1B (IL1B) gene and their association between ILD with antisynthetase autoantibodies, as well as IL-1β serum levels. The most frequent antisynthetase autoantibody was anti-Jo1. The most frequent tomographic pattern was non-specific interstitial pneumonia, whereas in the anti-Jo1 subjects, it was organized pneumonia. Anti-Jo1 patients tend to have more significant arthritis, and Raynaud phenomenon have higher levels of creatinine phosphokinase. In the IL1B gene, the GG genotype and G allele of rs1143634 [odds ratio (OR) = 2.21 and OR = 2.60, respectively, p < 0.05] are associated with an increased risk, as well as with the dominant and recessive models (p < 0.05). This finding is maintained after logistic regression analysis adjusting for potential confounding variables (p < 0.05). Subjects with the rs16944/AG heterozygous genotype had higher serum levels of IL-1β compared to homozygous (p < 0.05). In conclusion, rs1143634 is associated with a higher risk of ASSD. Also, the GA genotype is associated with higher levels of IL-1β in ASSD patients.
Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield
Authors: Dang Ke , Gong Xiangwei , Zhao Guan , Wang Honglu , Ivanistau Aliaksandr , Feng Baili
Published: November 2020

Intercropping of cereals and legumes has been used in modern agricultural systems, and the soil microorganisms associated with legumes play a vital role in organic matter decomposition and nitrogen (N) fixation. This study investigated the effect of intercropping on the rhizosphere soil microbial composition and structure and how this interaction affects N absorption and utilization by plants to improve crop productivity. Experiments were conducted to analyze the rhizosphere soil microbial diversity and the relationship between microbial composition and N assimilation by proso millet (Panicum miliaceum L.) and mung bean (Vigna radiata L.) from 2017 to 2019. Four different intercropping row arrangements were evaluated, and individual plantings of proso millet and mung bean were used as controls. Microbial diversity and community composition were determined through Illumina sequencing of 16S rRNA and internal transcribed spacer (ITS) genes. The results indicated that intercropping increased N levels in the soil–plant system and this alteration was strongly dependent on changes in the microbial (bacterial and fungal) diversities and communities. The increase in bacterial alpha diversity and changes in unique operational taxonomic unit (OTU) numbers increased the soil N availability and plant N accumulation. Certain bacterial taxa (such as Proteobacteria) and fungal taxa (such as Ascomycota) were significantly altered under intercropping and showed positive responses to increased N assimilation. The average grain yield of intercropped proso millet increased by 13.9–50.1% compared to that of monoculture proso millet. Our data clearly showed that intercropping proso millet with mung bean altered the rhizosphere soil microbial diversity and community composition; thus, this intercropping system represents a potential mechanism for promoting N assimilation and increasing grain yield.
Repetitive Religious Chanting Invokes Positive Emotional Schema to Counterbalance Fear: A Multi-Modal Functional and Structural MRI Study
Authors: Gao Junling , Skouras Stavros , Leung Hang Kin , Wu Bonnie Wai Yan , Wu Huijun , Chang Chunqi , Sik Hin Hung
Published: November 2020

Introduction

During hard times, religious chanting/praying is widely practiced to cope with negative or stressful emotions. While the underlying neural mechanism has not been investigated to a sufficient extent. A previous event-related potential study showed that religious chanting could significantly diminish the late-positive potential induced by negative stimuli. However, the regulatory role of subcortical brain regions, especially the amygdala, in this process remains unclear. This multi-modal MRI study aimed to further clarify the neural mechanism underlying the effectiveness of religious chanting for emotion regulation.

Methodology

Twenty-one participants were recruited for a multi-modal MRI study. Their age range was 40–52 years, 11 were female and all participants had at least 1 year of experience in religious chanting. The participants were asked to view neutral/fearful pictures while practicing religious chanting (i.e., chanting the name of Buddha Amitābha), non-religious chanting (i.e., chanting the name of Santa Claus), or no chanting. A 3.0 T Philips MRI scanner was used to collect the data and SPM12 was used to analyze the imaging data. Voxel-based morphometry (VBM) was used to explore the potential hemispheric asymmetries in practitioners.

Results

Compared to non-religious chanting and no chanting, higher brain activity was observed in several brain regions when participants performed religious chanting while viewing fearful images. These brain regions included the fusiform gyrus, left parietal lobule, and prefrontal cortex, as well as subcortical regions such as the amygdala, thalamus, and midbrain. Importantly, significantly more activity was observed in the left than in the right amygdala during religious chanting. VBM showed hemispheric asymmetries, mainly in the thalamus, putamen, hippocampus, amygdala, and cerebellum; areas related to skill learning and biased memory formation.

Conclusion

This preliminary study showed that repetitive religious chanting may induce strong brain activity, especially in response to stimuli with negative valence. Practicing religious chanting may structurally lateralize a network of brain areas involved in biased memory formation. These functional and structural results suggest that religious chanting helps to form a positive schema to counterbalance negative emotions. Future randomized control studies are necessary to confirm the neural mechanism related to religious chanting in coping with stress and negative emotions.
Risk of Recurrence of Chronic Subdural Hematomas After Surgery: A Multicenter Observational Cohort Study
Authors: Cofano Fabio , Pesce Alessandro , Vercelli Giovanni , Mammi Marco , Massara Armando , Minardi Massimiliano , Palmieri Mauro , D'Andrea Giancarlo , Fronda Chiara , Lanotte Michele Maria , Tartara Fulvio , Zenga Francesco , Frati Alessandro , Garbossa Diego
Published: November 2020

Background: Chronic Subdural Hematoma (CSDH) is a common condition in the elderly population. Recurrence rates after surgical evacuation range from 5 to 30%. Factors predicting recurrence remain debated and unclear.

Objective: To identify factors associated with increased risk of recurrence.

Methods: Cases of CSDHs that underwent surgical treatment between 2005 and 2018 in the Neurosurgery Units of two major Italian hospitals were reviewed. Data extracted from a prospectively maintained database included demographics, laterality, antithrombotic therapy, history of trauma, corticosteroid therapy, preoperative and postoperative symptoms, type of surgical intervention, use of surgical drain, and clinical outcomes.

Results: A total of 1313 patients was analyzed. The overall recurrence rate was 10.1%. The risk of recurrence was not significantly different between patients with unilateral or bilateral CSDH (10.4 vs. 8.8%, p = 0.39). The risk of recurrence was higher in patients that underwent surgical procedure without postoperative drainage (16.1 vs. 5.4%, p < 0.01). No relationship was found between recurrence rates and therapy with antithrombotic drugs (p = 0.97). The risk of recurrence was increasingly higher considering craniostomy, craniectomy, and craniotomy (9.3, 11.3, and 18.9%, respectively, p = 0.013). Lower recurrence rates following Dexamethasone therapy were recorded (p = 0.013).

Conclusion: No association was found between the risk of recurrence of CSDH after surgical evacuation and age, use of antithrombotic medication, or laterality. Burr-hole craniostomy was found to be associated with lower recurrence rates, when compared to other surgical procedures. Placement of surgical drain and Dexamethasone therapy were significantly associated with reduced risk of recurrence of CSDHs.
Effects of COVID-19 Pandemic Confinement in Patients With Cognitive Impairment
Authors: Barguilla Ainara , Fernández-Lebrero Aida , Estragués-Gázquez Isabel , García-Escobar Greta , Navalpotro-Gómez Irene , Manero Rosa María , Puente-Periz Víctor , Roquer Jaume , Puig-Pijoan Albert
Published: November 2020

Introduction: State of emergency caused by COVID-19 pandemic and subsequent lockdown hit Spain on 14th March 2020 and lasted until 21st June 2020. Social isolation measures were applied. Medical attention was focused on COVID-19. Primary and social care were mainly performed by telephone. This exceptional situation may affect especially vulnerable patients such as people living with dementia. Our aim was to describe the influence of restrictive measures on patients living with mild cognitive decline and dementia evaluating SARS-CoV2 infection, changes in routines, cognitive decline stage, neuropsychiatric symptoms, delirium, falls, caregiver stress, and access to sanitary care.

Materials and Methods: We gathered MCI and dementia patients with clinical follow-up before and after confinement from DegMar registry (Hospital del Mar). A telephone ad-hoc questionnaire was administered. Global status was assessed using CDR scale. Changes in neuropsychiatric symptoms were assessed by Neuropsychiatric Inventory (NPI) and retrospective interview for pre-confinement base characteristics.

Results: We contacted a total of 60 patients, age 75.4 years ± 5,192. 53.3% were women. Alzheimer's Disease (41.7%) and Mild Cognitive Impairment (25%) were the most prevalent diagnosis. Remaining cases included different dementia disorders. A total of 10% of patients had been diagnosed with SARS-CoV-2. During confinement 70% of patients abandoned previous daily activities, 60% had cognitive worsening reported by relatives/caretakers, 15% presented delirium episodes, and 13% suffered increased incidence of falls. Caregivers reported an increased burden in 41% cases and burnout in 11% cases. 16% reported difficulties accessing medical care, 33% received medical phone assistance, 20% needed emergency care and 21% had changes in psychopharmacological therapies. Neuropsychiatric profile globally worsened (p < 0.000), also in particular items like agitation (p = 0.003), depression (p < 0.000), anxiety (p < 0.000) and changes in appetite (p = 0.004).

Conclusion: SARS-CoV2-related lockdown resulted in an important effect over social and cognitive spheres and worsening of neuropsychiatric traits in patients living with mild cognitive decline and dementia. Although the uncertainty regarding the evolution of the pandemic makes strategy difficult, we need to reach patients and caregivers and develop adequate strategies to reinforce and adapt social and health care.
Studying the Factors of Human Carotid Atherosclerotic Plaque Rupture, by Calculating Stress/Strain in the Plaque, Based on CEUS Images: A Numerical Study
Authors: Li Zhenzhou , Wang Yongfeng , Wu Xinyin , Liu Xin , Huang Shanshan , He Yi , Liu Shuyu , Ren Lijie
Published: November 2020

Carotid plaque neovascularization is one of the major factors for the classification of vulnerable plaque, but the axial force effects of the pulsatile blood flow on the plaque with neovessel and intraplaque hemorrhage was unclear. Together with the severity of stenosis, the fibrous cap thickness, large lipid core, and the neovascularization followed by intraplaque hemorrhage (IPH) have been regarded as high-risk features of plaque rupture. In this work, the effects of these factors were evaluated on the progression and rupture of the carotid atherosclerotic plaques. Five geometries of carotid artery plaque were developed based on contrast-enhanced ultrasound (CEUS) images, which contain two types of neovessel and IPH, and geometry without neovessel and IPH. A one-way fluid-structure interaction model was applied to compute the maximum principal stress and strain in the plaque. For that hyper-elastic and non-linear material, Yeoh 3rd Order strain energy density function was used for components of the plaque. The simulation results indicated that the maximum principal stress of plaque in the carotid artery was higher when the degree of the luminal stenosis increased and the thickness of the fibrous cap decreased. The neovessels within the plaque could introduce a 2.5% increments of deformation in the plaque under the pulsatile blood flow pressure. The IPH also contributed to the increased risk of plaque rupture that a gain of stress was 8.983, 14.526, and 34.47 kPa for the plaque with 50, 65, and 75%, respectively, when comparing stress in the plaque with IPH distributed at the middle to the shoulder of the plaque. In conclusion, neovascularization in the plaque could reduce the stability of the plaque by increasing the stress within the plaque. Also, the risk of plaque rupture increased when large luminal stenosis, thin fibrous cap, and IPH were observed.
A Prognostic Nomogram Model Based on mRNA Expression of DNA Methylation-Driven Genes for Gastric Cancer
Authors: Chen Zuhua , Liu Bo , Yi Minxiao , Qiu Hong , Yuan Xianglin
Published: November 2020

Purpose

The exploration and interpretation of DNA methylation-driven genes might contribute to molecular classification, prognostic prediction and therapeutic choice. In this study, we built a prognostic risk model via integrating analysis of the transcriptome and methylation profile for patients with gastric cancer (GC).

Methods

The mRNA expression profiles, DNA methylation profiles and corresponding clinicopathological information of 415 GC patients were downloaded from The Cancer Genome Atlas (TCGA). Differential expression and correlation analysis were performed to identify DNA methylation-driven genes. The candidate genes were selected by univariate Cox regression analyses followed by the least absolute shrinkage and selection operator (LASSO) regression. A prognostic risk nomogram model was then built together with clinicopathological parameters.

Results

5 DNA methylation-driven genes (CXCL3, F5, GNAI1, GAMT and GHR) were identified by integrated analyses and selected to construct the prognostic risk model with clinicopathological parameters. High expression and low DNA hypermethylation of F5, GNAI1, GAMT and GHR, as well as low expression and high DNA hypomethylation of CXCL3 were significantly associated with poor prognosis rates, respectively. The high-risk group showed a significantly shorter prognosis than the low-risk group in the TCGA dataset (HR = 0.212, 95% CI = 0.139–0.322, P = 2e-15). The final nomogram model showed high predictive efficiency and consistency in the training and validation group.

Conclusion

We construct and validate a prognostic nomogram model for GC based on five DNA methylation-driven genes with high performance and stability. This nomogram model might be a powerful tool for prognosis evaluation in the clinic and also provided novel insights into the epigenetics in GC.
Prognostic Significance of Preoperative Neutrophil-to-Lymphocyte Ratio in Patients With Meningiomas
Authors: Kuranari Yuki , Tamura Ryota , Tsuda Noboru , Kosugi Kenzo , Morimoto Yukina , Yoshida Kazunari , Toda Masahiro
Published: November 2020

Background

Meningiomas are the most common benign intracranial tumors. However, even WHO grade I meningiomas occasionally show local tumor recurrence. Prognostic factors for meningiomas have not been fully established. Neutrophil-to-lymphocyte ratio (NLR) has been reported as a prognostic factor for several solid tumors. The prognostic value of NLR in meningiomas has been analyzed in few studies.

Materials and Methods

This retrospective study included 160 patients who underwent surgery for meningiomas between October 2010 and September 2017. We analyzed the associations between patients’ clinical data (sex, age, primary/recurrent, WHO grade, extent of removal, tumor location, peritumoral brain edema, and preoperative laboratory data) and clinical outcomes, including recurrence and progression-free survival (PFS).

Results

Forty-four meningiomas recurred within the follow-up period of 3.8 years. WHO grade II, III, subtotal removal, history of recurrence, Ki-67 labeling index ≥3.0, and preoperative NLR value ≥2.6 were significantly associated with shorter PFS (P < 0.001, < 0.001, 0.002, < 0.001, and 0.015, respectively). Furthermore, NLR ≥ 2.6 was also significantly associated with shorter PFS in a subgroup analysis of WHO grade I meningiomas (P = 0.003). In univariate and multivariate analyses, NLR ≥2.6 remained as a significant predictive factor for shorter PFS in patients with meningioma (P = 0.014).

Conclusions

NLR may be a cost-effective and novel preoperatively usable biomarker in patients with meningiomas.
Initial Gamma Knife Radiosurgery for Large or Documented Growth Asymptomatic Meningiomas: Long-Term Results From a 27-Year Experience
Authors: Fu Junyi , Wu Lisha , Peng Chao , Yang Xin , You Hongji , Cao Linhui , Deng Yinhui , Yu Jinxiu
Published: November 2020

Objective

The aims of this study were to investigate the long-term outcomes of initial Gamma Knife radiosurgery (GKRS) for large (≥20 mm) or documented growth asymptomatic meningiomas.

Design and Methods

This was a single-center retrospective study. Fifty-nine patients with large (≥20 mm) or documented growth asymptomatic meningiomas undergoing initial GKRS were enrolled. The median age was 56 (range, 27–83) years. The median time of follow-up was 66.8 (range, 24.6–245.6) months, and the median tumor margin dose was 13.0 Gy (range, 11.6–22.0 Gy).

Results

Tumors shrunk in 35 patients (59.3%) and remained stable in 23 (39.0%). One patient (1.7%) experienced radiological progression at 54 months after GKRS. The PFS was 100%, 97%, and 97% at 3, 5, and 10 years, respectively. Nine patients (15.3%) occurred new neurological symptoms or signs at a median time of 8.1 (range, 3.0–81.6) months. The symptom PFS was 90% and 78% at 5 and 10 years, respectively. Fifteen patients (25.4%) occurred peritumoral edema (PTE) at a median time of 7.2 (range, 2.0–81.6) months. One patient underwent surgical resection for severe PTE. In univariate and multivariate analysis, Only tumor size (≥25 mm) and maximum dose (≥34 Gy) were significantly associated with PTE [hazard ratio (HR)= 3.461, 95% confidence interval (CI)=1.157-10.356, p=0.026 and HR=3.067, 95% CI=1.068-8.809, P=0.037, respectively].

Conclusions

In this study, initial GKRS can provide a high tumor control rate as well as an acceptable rate of complications in large or documented growth asymptomatic meningiomas. GKRS may be an alternative initial treatment for asymptomatic meningiomas.
Comprehensive Transcriptomic Analysis Reveals Dysregulated Competing Endogenous RNA Network in Endocrine Resistant Breast Cancer Cells
Authors: Gao Liang , Shen Kunwei , Yin Ni , Jiang Min
Published: November 2020

Background

Tamoxifen and fulvestrant, both approved for endocrine therapy, have remarkably increased the prognosis of hormone receptor-positive breast cancer patients. However, acquired resistance to endocrine therapy greatly reduces its clinical efficacy. Accumulating evidence suggests a pivotal role of non-coding RNAs (ncRNAs) in breast cancer endocrine resistance, but the specific functions of ncRNAs in tamoxifen and fulvestrant resistance remain largely unknown.

Methods

Microarray analysis was performed for endocrine therapy sensitive (MCF-7), tamoxifen-resistant (LCC2), and dual tamoxifen and fulvestrant-resistant (LCC9) breast cancer cells. Gene ontology and pathway analysis were conducted for functional prediction of the unannotated differentially expressed ncRNAs. Competing endogenous RNA regulatory networks were constructed.

Results

We discovered a total of 3,129 long non-coding RNAs (lncRNAs), 13,556 circular RNAs (circRNAs), 132 microRNAs, and 3358 mRNAs that were significantly differentially expressed. We constructed co-expression networks for lncRNA-mRNA, circRNA-mRNA, and microRNA-mRNA. In addition, we established lncRNA-microRNA-mRNA and circRNA-microRNA-mRNA regulatory networks to depict ncRNA crosstalk and transcriptomic regulation of endocrine resistance.

Conclusions

Our study delineates a comprehensive profiling of ncRNAs in tamoxifen and fulvestrant resistant breast cancer cells, which enriches our understanding of endocrine resistance and sheds new light on identifying novel endocrine resistance biomarkers and potential therapeutic targets to overcome endocrine resistance.
The Therapeutic Potential of the Essential Oil of Thymbra capitata (L.) Cav., Origanum dictamnus L. and Salvia fruticosa Mill. And a Case of Plant-Based Pharmaceutical Development
Authors: Pirintsos S. A. , Bariotakis M. , Kampa M. , Sourvinos G. , Lionis C. , Castanas E.
Published: November 2020

This review performs a comprehensive assessment of the therapeutic potential of three native herbs of Crete (Thymbra capitata (L.) Cav., Salvia fruticosa Mill. and Origanum dictamnus L.), their phytochemical constituents, health benefits and issues relevant to their safety, within a translational context. Issues discussed comprise: 1) Ethnopharmacological uses of the three herbs, reviewed through an extensive search of the literature; 2) Systematic analysis of the major phytochemical constituents of each plant, and their medicinal properties; 3) To what extent could the existing medicinal properties be combined and produce an additive or synergistic effect; 4) Possible safety issues. We conclude with a specific example of the use of a combination of the essential oils of these plants as an effective anti-viral product and the experience gained in a case of a plant-based pharmaceutical development, by presenting the major steps and the continuum of the translational chain.
Tetrahydrocannabinol-Rich Extracts From Cannabis Sativa L. Improve Glucose Consumption and Modulate Metabolic Complications Linked to Neurodegenerative Diseases in Isolated Rat Brains
Authors: Erukainure Ochuko L. , Matsabisa Motlalepula G. , Salau Veronica F. , Islam Md. Shahidul
Published: November 2020

Reduced brain glucose consumption arising from impaired glucose uptake and utilization has been linked to the pathogenesis and complications of neurodegenerative diseases. The ability of Cannabis sativa L. tetrahydrocannabinol (THC)-rich extracts to stimulate brain glucose uptake and utilization as well as its modulatory effect on gluconeogenesis, antioxidative, purinergic and cholinergic activities were investigated in isolated rats’ brains. C. sativa leaves were sequentially extracted to yield the hexane and dichloromethane extracts. The extracts were incubated at 37°C with freshly harvested brains in the presence of glucose for 2 h. The control consisted of incubation without the extracts, while brains without the extracts and glucose served as the normal control. Metformin was used as the standard drug. C. sativa extracts caused a significant (p < 0.05) increase in brain glucose uptake, with concomitant elevation of glutathione level, superoxide dismutase, catalase, and ecto-nucleoside triphosphate diphosphohydrolase activities compared to the controls. Incubation with C. sativa extracts also led to depletion in malondialdehyde and nitric oxide levels, acetylcholinesterase, butyrylcholinesterase, glucose 6-phosphatase and fructose-1,6-biphosphatase activities. GC-MS analysis of the extracts revealed the presence of THC. In silico analysis predicted THC to be permeable across the blood-brain-barrier. THC was also predicted to have an oral LD50 and toxicity class values of 482 mg/kg and 4 respectively. These results indicate that C. sativa improves glucose consumption with concomitant suppression of oxidative stress and cholinergic dysfunction, and modulation of purinergic and gluconeogenic activities in brain tissues
The Maximum Principle for Variable-Order Fractional Diffusion Equations and the Estimates of Higher Variable-Order Fractional Derivatives
Authors: Xue Guangming , Lin Funing , Su Guangwang
Published: November 2020

In this paper, the maximum principle of variable-order fractional diffusion equations and the estimates of fractional derivatives with higher variable order are investigated. Firstly, we deduce the fractional derivative of a function of higher variable order at an arbitrary point. We also give an estimate of the error. Some important inequalities for fractional derivatives of variable order at arbitrary points and extreme points are presented. Then, the maximum principles of Riesz-Caputo fractional differential equations in terms of the multi-term space-time variable order are proved. Finally, under the initial-boundary value conditions, it is verified via the proposed principle that the solutions are unique, and their continuous dependance holds.
Review on the Stability of the Peregrine and Related Breathers
Authors: Alejo Miguel A. , Fanelli Luca , Muñoz Claudio
Published: November 2020

In this note, we review stability properties in energy spaces of three important nonlinear Schrödinger breathers: Peregrine, Kuznetsov-Ma, and Akhmediev. More precisely, we show that these breathers areunstableaccording to a standard definition of stability. Suitable Lyapunov functionals are described, as well as their underlying spectral properties. As an immediate consequence of the first variation of these functionals, we also present the corresponding nonlinear ODEs fulfilled by these nonlinear Schrödinger breathers. The notion of global stability for each breather mentioned above is finally discussed. Some open questions are also briefly mentioned.
Development of a Yellow-Seeded Stable Allohexaploid Brassica Through Inter-Generic Somatic Hybridization With a High Degree of Fertility and Resistance to Sclerotinia sclerotiorum
Authors: Kumari Preetesh , Singh Kaushal Pratap , Kumar Sundip , Yadava Devendra Kumar
Published: November 2020

The Brassica coenospeceis have treasure troves of genes that could be beneficial if introgressed into cultivated Brassicas to combat the current conditions of climate change. Introducing genetic variability through plant speciation with polyploidization is well documented, where ploidy augmentation of inter-generic allohexaploids using somatic hybridization has significantly contributed to genetic base broadening. Sinapis alba is a member of the Brassicaceae family that possesses valuable genes, including genes conferring resistance to Sclerotinia sclerotiorum, Alternaria brassicae, pod shattering, heat, and drought stress. This work aimed to synthesize stable allohexaploid (AABBSS) Brassica while incorporating the yellow-seed trait and resistance to S. sclerotiorum stem rot. The two fertile and stable allohexaploids were developed by polyethylene glycol mediated protoplast fusions between Brassica juncea (AABB) and S. alba (SS) and named as JS1 and JS2. These symmetric hybrids (2n = 60) were validated using morphological and molecular cytology techniques and were found to be stable over consecutive generations. The complete chromosome constitution of the three genomes was determined through genomic in situ hybridization of mitotic cells probed with S. alba genomic DNA labeled with fluorescein isothiocyanate. These two allohexaploids showed 24 hybridization signals demonstrating the presence of complete diploid chromosomes from S. alba and 36 chromosomes from B. juncea. The meiotic pollen mother cell showed 30 bivalent sets of all the 60 chromosomes and none of univalent or trivalent observed during meiosis. Moreover, the backcross progeny 1 plant revealed 12 hybridization signals out of a total of 48 chromosome counts. Proper pairing and separation were recorded at the meiotic metaphase and anaphase, which proved the stability of the allohexaploid and their backcross progeny. When screening, the allohexaploid (JS2) of B. juncea and S. alba displayed a high degree of resistance to S. sclerotiorum rot along with a half-yellow and half-brown (mosaic) seed coat color, while the B. juncea and S. alba allohexaplopid1 (JS1) displayed a yellow seed coat color with the same degree of resistance to Sclerotinia rot.
Can We Apply WHOQOL-AGE to Asian Population? Verifying Its Factor Structure and Psychometric Properties in a Convenience Sample From Taiwan
Authors: Lin Chung-Ying , Wang Jung-Der , Liu Li-Fan
Published: November 2020

Objectives: To translate and validate a recently developed quality of life instrument (WHOQOL-AGE) on geriatric population into Chinese.

Method: Using cross-sectional observational design, the WHOQOL-AGE was conducted among older people through interview. Confirmatory factor analysis (CFA) was used to examine the factor structure and multigroup CFA used to examine the measurement invariance.

Results: Through convenience sampling, 522 older adults (mean age = 73.42) participated in the study. Among them, 194 were males, 213 had an educational level at primary school or below, 398 were residing in the community, and 307 were aged 70 years or above. A bifactor structure (items Q1–Q8 are embedded in the factor 1; items Q9–Q13 embedded in the factor 2; and all the items embedded in an additional construct of QoL) was confirmed by the CFA in both the entire sample (χ2 = 25.4; df = 51; p = 0.999) and the subgroup sample with age 70 years or above (χ2 = 25.28; df = 51; p = 1.000). Multigroup CFAs results supported the measurement invariance for the WHOQOL-AGE across genders, having different educational levels, living in different settings and age groups. It also shows good known-groups validity.

Conclusions: The promising psychometric properties of the WHOQOL-AGE were found in our convenience sample of older Taiwanese. The supported measurement invariance indicates that the older people in different conditions of gender, educational level, and living setting interpret the WHOQOL-AGE similarly. However, our results should be interpreted with cautious because of the sample representativeness.
Effects of Pandemics-Related Uncertainty on Household Consumption: Evidence From the Cross-Country Data
Authors: Wu Shuiting
Published: November 2020

The COVID-19 pandemic has affected various macroeconomic indicators. Given this backdrop, this research investigates the effects of the pandemics-related uncertainty on household consumption. For this purpose, we construct a simple theoretical model to study the effects of the pandemics-related uncertainty on household consumption. To estimate the theoretical model, we consider the panel dataset of 138 countries for the period from 1996 to 2017. We also use the Pandemic Uncertainty Index to measure the pandemics-related uncertainty. The theoretical model and the empirical findings from the Feasible Generalized Least Squares (FGLS) estimations indicate that the gross fixed capital formation, government consumption, balance of trade, and the Pandemic Uncertainty Index negatively affect household consumption. The results are also valid in the panel dataset of 42 high-income economies and the remaining 96 emerging economies.
Contaminant Removal and Precious Metal Recovery by Lateral Channel Filtration in Mining-Impacted Alluvial Floodplains
Authors: Johnson William P. , Pazmiño Eddy , Frederick Logan , Ron Cesar , Chica Andrea , Tapia Luis
Published: November 2020

Water quality impacts from artisanal and small-scale alluvial (placer) gold mining operations occur in developing economies across several continents including Asia, Africa, and South America. They often occur in remote and/or resource-poor settings in which mitigation strategies must contend with extreme seasonal variation in river flow as well as the economic incentive to periodically churn (mine) alluvial floodplains without riparian restoration. A novel strategy addressing these constraints is herein explored which employs the alluvial floodplain for filtration-driven removal of particulate contaminants and gold from streams. This process of lateral channel filtration is explored in the Rio Nambija of southern Ecuador, in terms of success in achieving the hydraulic objective of passively driven lateral flow, and the corresponding removal of particulate contaminants (e.g., total mercury, lead, iron, and manganese) by filtration. Accumulation of gold was examined to evaluate whether incorporation of this proposed practice in alluvial (placer) mining can reduce particle-bound contaminants in streams and simultaneously increase economic benefit. Excavation of channels lateral to mining-impacted streams was shown to achieve the hydraulic, water quality, and economic (gold accumulation) objectives. The modest flow capture for any given lateral segment, along with the months-long timescale associated with economic gold accumulation and clogging by suspended solids, dictate a “cultivation” process whereby multiple lateral segments are worked annually.
Esophageal Rhabdoid-Like Tumor: A Rare Disease With Aggressive Clinical Behavior
Authors: Lovece Andrea , Bernardi Daniele , Bruni Barbara , Asti Emanuele , Clemente Claudio , Bonavina Luigi
Published: November 2020

Background: Malignant rhabdoid tumor is a kidney childhood tumor with aggressive clinical behavior and a wide spectrum of histologic, immunophenotypic, and cytogenetic findings. Extra-renal rhabdoid tumors have been reported in the brain, breast, liver, pancreas, bladder, vulva, prostate, and colon. To date, only nine cases of esophageal rhabdoid tumors have been described, all in patients over 50-year old. We add to the current literature the case of an esophageal, poorly differentiated rhabdoid tumor occurring in a young man.

Case Report: A 24-year-old man was referred for progressive dysphagia, retrosternal pain, nausea, and food regurgitation. Esophagogastroduodenoscopy showed an obstructing neoplastic lesion of the distal esophagus associated with Barrett's esophagus. Biopsies revealed undifferentiated esophageal cancer with epithelial morphology and immunohistochemistry positive for CK pan, CK 7 e CK 8-18. Minimally invasive esophagectomy and extended lymphadenectomy was performed. Histopathology showed a poorly differentiated tumor, with morphologic characteristics of rhabdoid tumor, central necrosis and transmural infiltration of the esophageal wall. Definitive immunohistochemistry was positive for vimentin, CD34, synaptophysin, and INI1.

Conclusion: Esophageal rhabdoid tumor is extremely rare and highly aggressive, with only few patients alive at 1 year follow-up, according to our review. Immunohistochemistry characterization is critical for diagnosis. Minimally invasive esophagectomy is an appealing and possibly less morbid option compared to open surgery. However, further research is needed to investigate the potential role of targeted immunotherapy.
Case Report: Intoxication in a Pig (Sus Scrofa Domesticus) After Transdermal Fentanyl Patch Ingestion
Authors: Sredenšek Jerneja , Bošnjak Maša , Lampreht Tratar Urša , Kosjek Tina , Cemazar Maja , Kržan Mojca , Seliškar Alenka
Published: November 2020

An experimental study on the effects of electroporation on pancreatic tissue was performed in pigs, and the fentanyl transdermal patch (FTP) was used postoperatively as part of multimodal pain management. Ingestion of an FTP, which resulted in fentanyl intoxication, was suspected 5 days after placement in one of the experimental pigs. The pig was first dysphoric, running in the stall, panting and vocalizing until it finally became depressed and it remained lying on the floor. Ingestion of an FTP was not observed but the fentanyl plasma concentration on the day of intoxication was 20.7 ng/ml, while at its peak after FTP administration it was only 0.492 ng/ml. The intoxication was successfully treated with a single intramuscular naloxone injection.
Conservation Agriculture Practices Can Improve Earthworm Species Richness and Abundance in the Semi-Arid Climate of Eastern Cape, South Africa
Authors: Mcinga Sixolise , Muzangwa Lindah , Janhi Kudzayi , Mnkeni Pearson Nyari Stephano
Published: November 2020

Earthworms play a pivotal role in the regulation of soil health. Studies that explore the effects of conservation agriculture (CA) principles on earthworms under the semi-arid climate of the central Eastern Cape (EC) of South Africa (SA) are limited. Therefore, this study investigated the effects of tillage, crop rotations, and residue management on earthworms’ abundance and species richness. The study design followed a split-split plot with three replicates. The main plot was allocated to tillage treatment, which had conventional tillage (CT) and no-tillage (no-till) as factors. Crop rotation treatment was allocated to a subplot, and had maize (Zea mays)–fallow–maize (MFM), maize–fallow–soybean (Glycine max) (MFS), maize–wheat (Triticum aestivum)–maize (MWM), and maize–wheat–soybean (MWS). Residue management was in the sub-subplot with residue retention and residue removal. The study was carried out over four cropping seasons: summer 2015–2016, winter 2016, spring 2016, and summer 2016–2017. The results showed that the genera Amynthas and Lumbricus, both belonging to the anecic group, and Dendrobaena, belonging to the epigeic group, were present. Earthworm species diversity and density were highest under no-till than under CT. Residue retention improved earthworm density regardless of tillage management. Rotations that had fallow periods recorded lower earthworm numbers as compared to continuous cropping systems where wheat was grown in winter. The study concluded that maize–wheat–soybean (MWS) rotation with residue retention results in the highest earthworm abundance and species richness.
Assessment of Drivers of Antimicrobial Use and Resistance in Poultry and Domestic Pig Farming in the Msimbazi River Basin in Tanzania
Authors: Kimera Zuhura I. , Frumence Gasto , Mboera Leonard E. G. , Rweyemamu Mark , Mshana Stephen E. , Matee Mecky I. N.
Published: November 2020

Uncontrolled use of drugs both in humans and animals coupled with environmental contamination exacerbate the development and spread of antimicrobial resistance. This paper assessed the drivers of antimicrobial use and resistance in poultry and domestic pig farming and the environment. Questionnaires, in-depth interviews, and focus group discussions (FGDs) were used to collect information regarding demographic characteristics, knowledge, practices, attitudes, and perceptions of the drivers of antimicrobial use and resistance in animal farming and the environment. We found a higher proportion of usage of veterinary antimicrobials for prophylactic purposes (87.6%) in animal farming, than for therapeutic purposes (80.5%). The degree of farming experience was significantly (p < 0.05) related to the knowledge on the source of antimicrobial use, methods used in disease diagnosis, access to veterinary services, stocking of antimicrobials at home, and presence of agriculture activities that involve the use of manure. Uncontrolled disposal of wastes from households, disposal of human and veterinary drugs, and weak implementation of the legal framework was identified as the major contributors to the environment. The high usage of veterinary antimicrobials and the environmental contamination identified requires multisectoral interventions, as well as a review of government strategies, policies, and regulations on antimicrobial use.
Genotypic Characteristics and Correlation of Epidemiology of Staphylococcus aureus in Healthy Pigs, Diseased Pigs, and Environment
Authors: Zhou Yuanyuan , Li Xinhui , Yan He
Published: November 2020

China is one of the largest producers of pigs and pork in the world. However, large-scale studies on pig-associated Staphylococcus aureus in relation to healthy pigs, diseased pigs and environment are scarce. The objective of the present study was to characterize and compare S. aureus isolates from healthy pigs, diseased pigs and environment through antimicrobial susceptibility testing, multiple locus sequence typing, spa typing, and antimicrobial resistance gene screening. Results showed all isolates were susceptible to linezolid and vancomycin. However, 66.7% (104/156) isolates were multidrug-resistant by displaying resistance to three or more antibiotics and high rates of resistance to penicillin, tetracycline, clindamycin, and clarithromycin were observed. Of the 20 multilocus sequence types (STs) identified among the isolates, ST9, ST188, and ST7 were most commonly isolated from healthy pigs and environment, while ST1 was most commonly isolated from diseased pigs. In total, 17 spa types were represented among the isolates, while t4792 was most commonly isolated from diseased pigs and t899, t189 were most commonly isolated from healthy pigs and environment. In conclusion, the genotypic and epidemiology characteristics observed among the isolates suggest pigs and pork could be important players in S. aureus dissemination.
Fractional-Order SIR Epidemic Model for Transmission Prediction of COVID-19 Disease
Authors: Kozioł Kamil , Stanisławski Rafał , Bialic Grzegorz
Published: November 2020

In this paper, the fractional-order generalization of the susceptible-infected-recovered (SIR) epidemic model for predicting the spread of the COVID-19 disease is presented. The time-domain model implementation is based on the fixed-step method using the nabla fractional-order difference defined by Grünwald-Letnikov formula. We study the influence of fractional order values on the dynamic properties of the proposed fractional-order SIR model. In modeling the COVID-19 transmission, the model’s parameters are estimated while using the genetic algorithm. The model prediction results for the spread of COVID-19 in Italy and Spain confirm the usefulness of the introduced methodology.
ARMONIA: A Unified Access and Metro Network Architecture
Authors: Kretsis Aristotelis , Sartzetakis Ippokratis , Soumplis Polyzois , Mitropoulou Katerina , Kokkinos Panagiotis , Nicopolitidis Petros , Papadimitriou Georgios , Varvarigos Emmanouel
Published: November 2020

We present a self-configured and unified access and metro network architecture, named ARMONIA. The ARMONIA network monitors its status, and dynamically (re-)optimizes its configuration. ARMONIA leverages software defined networking (SDN) and network functions virtualization (NFV) technologies. These technologies enable the access and metro convergence and the joint and efficient control of the optical and the IP equipment used in these different network segments. Network monitoring information is collected and analyzed utilizing machine learning and big data analytics methods. Dynamic algorithms then decide how to adapt and dynamically optimize the unified network. The ARMONIA network enables unprecedented resource efficiency and provides advanced virtualization services, reducing the capital expenditures (CAPEX) and operating expenses (OPEX) and lowering the barriers for the introduction of new services. We demonstrate the benefits of the ARMONIA network in the context of dynamic resource provisioning of network slices. We observe significant spectrum and equipment savings when compared to static overprovisioning.
Parametric Assessment on the Advanced Exergy Performance of a CO2 Energy Storage Based Trigeneration System
Authors: Sun Wenxu , Liu Zhan
Published: November 2020

In this paper, conventional and advanced exergy analyses are comprehensively introduced on an innovative transcritical CO2 energy storage based trigeneration system. Conventional exergy analysis can quantify in an independent way the component exergy destruction. However, the advanced technology is able to evaluate the interactions among components and identify the tangible promotion potential by allowing for the technical and economic limitations. In this method, the component exergy destruction is separated into avoidable and unavoidable parts, as well as the endogenous/exogenous parts. Calculation of the split parts is carried out by utilizing the thermodynamic cycle-based approach. Results coming from conventional exergy analysis indicate that the first three largest exergy destructions are given by cold storage, compressor 1, and heat exchanger 3. However, advanced analysis results demonstrate that the cold storage, compressor 1, and compressor 2 should be given the first improvement priority in sequence by depending on the avoidable exergy destruction. The turbine efficiency produces a higher impact on overall exergy destruction than compressor efficiency. The pinch temperature in cold storage causes the highest effect on exergy destruction amongst all the heat exchangers. There exists an optimum value in the compressor inlet pressure and ambient temperature.