bluuebunny/crossref_metadata_2025 huggingface dataset:
Comment on Huang et al. Colistin Monotherapy versus Colistin plus Meropenem Combination Therapy for the Treatment of Multidrug-Resistant Acinetobacter baumannii Infection: A Meta-Analysis. J. Clin. Med. 2022, 11, 3239
Authors: Daitch Vered , Paul Mical , Leibovici Leonard
Published: November 2022

In the recently published meta-analysis titled “Colistin Monotherapy versus Colistin plus Meropenem Combination Therapy for the Treatment of Multidrug-Resistant Acinetobacter baumannii Infection: A Meta-Analysis”, Huang et al. compared the efficacy and safety of treatment with colistin monotherapy versus colistin plus meropenem combination therapy in patients with drug-resistant Acinetobacter baumannii infection [...]
Clinical Trials of Stem Cell Therapy in Japan: The Decade of Progress under the National Program
Authors: Enosawa Shin
Published: November 2022

Stem cell therapy is a current world-wide topic in medical science. Various therapies have been approved based on their effectiveness and put into practical use. In Japan, research and development-related stem cell therapy, generally referred to as regenerative medicine, has been led by the government. The national scheme started in 2002, and support for the transition to clinical trials has been accelerating since 2011. Of the initial 18 projects that were accepted in the budget for preclinical research, 15 projects have begun clinical trials so far. These include the transplantation of retinal, cardiac, and dopamine-producing cells differentiated from human induced pluripotent stem (iPS) cells and hepatocyte-like cells differentiated from human embryonic stem (ES) cells. The distinctive feature of the stem cell research in Japan is the use of iPS cells. A national framework was also been set-up to attain the final goal: health insurance coverage. Now, insurance covers cell transplantation therapies for the repair and recovery of damaged skin, articular cartilage, and stroke as well as therapies introduced from abroad, such as allogeneic mesenchymal stem cells for graft-versus-host disease and chimeric antigen receptor-T (CAR-T) cell therapy. To prepare this review, original information was sought from Japanese authentic websites, which are reliable but a little hard to access due to the fact of multiple less-organized databases and the language barrier. Then, each fact was corroborated by citing its English version or publication in international journals as much as possible. This review provides a summary of progress over the past decade under the national program and a state-of-the-art factual view of research activities, government policy, and regulation in Japan for the realization of stem cell therapy.
Role of Sport Activity on Quality of Life in Charcot-Marie-Tooth 1A Patients
Authors: Pazzaglia Costanza , Padua Luca , Stancanelli Claudia , Fusco Augusto , Loreti Claudia , Castelli Letizia , Imbimbo Isabella , Giovannini Silvia , Coraci Daniele , Vita Gian , Vita Giuseppe
Published: November 2022

The present study aims to investigate the benefits induced by physical activity/practiced sport in Charcot-Marie-Tooth 1A (CMT1A). Patients were divided into sport and no-sport groups according to their sports performance habit. Thirty-one patients were enrolled, of which 14 practiced sports and 17 did not. Clinical assessments were administered to evaluate disability, self-esteem, depression, quality of life, and pain. Statistical analysis revealed significant differences in terms of gender in the no-sport group compared to the sport group (p = 0.04). Regarding the quality of life, physical function (p = 0.001), general health (p = 0.03), social function (p = 0.04), and mental health (p = 0.006) showed better patterns in the sport group than no-sport group. Moreover, neuropathic pain was reduced in the sport group according to the Neuropathic Pain Symptom Inventory (p = 0.001) and ID-PAIN (p = 0.03). The other administered questionnaires showed no significant differences. Our study confirms that CMT1A patients, who practice sports, with a similar severity of disability, may have a better physical quality of life while suffering less neuropathic pain than their peers who do not practice sports. Results recommend the prescription of sport in CMT1A patients.
Impact of the First and Second Wave of the SARS-CoV-2 Pandemic on Severe Trauma and the Care Structures in the German TraumaNetzwerk DGU®
Authors: Colcuc Christian , Fischer Sebastian , Leimkühler Philipp , Miersbach Marco , Lefering Rolf , DGU TraumaRegister , Wähnert Dirk , Vordemvenne Thomas , Grüneweller Niklas
Published: November 2022

(1) Background: The aim of this study was to investigate the effects of the pandemic on transfer rates of severely injured patients within the German TraumaNetzwerk of the DGU. Furthermore, cause of accident, rescue times, and trauma cases are compared to pre-pandemic times. (2) Methods: For this investigation patients documented in the TraumaRegister DGU® from 2018 to 2020 were analyzed. The years 2018 and 2019 served as a comparison to 2020, the first COVID-19 pandemic year. All primary admissions and transfers were included if treated on an intensive care unit. (3) Results: Demographics (age, sex) and injury severity in 2020 were comparable with 2018/2019. In 2020, a significant decrease (3.7%) in car accidents was found. In contrast, a significant increase (3.2%) in bicycle accidents was seen. During the second wave, there was a significant burden of COVID-19 patients on hospitals. In this time, we found a significant increase in early transfers of trauma patients primarily from small level 3 to large level 1 centers. There was also a small but significant increase in rescue time, especially during the 2nd wave. (4) Conclusions: Our data confirm the importance of the network structures established in the TraumaNetzwerk DGU®, especially during the pandemic. The established structures allow smaller hospitals to spread their resources and prevent internal collapse. Therefore, the structures of the TraumaNetzwerk DGU® play a prominent role in stabilizing the healthcare system by helping to maintain both surgical and critical care capacity and providing adequate emergency care.
Conservative Therapies for TMJ Closed Lock: A Randomized Controlled Trial
Authors: Di Giacomo Paola , Di Paolo Carlo , Qorri Erda , Gatto Roberto , Manes Gravina Giovanni , Falisi Giovanni
Published: November 2022

Background. Acute anterior disc displacement without reduction (ADDWoR) is characterized by permanent TMJ disc displacement, pain and functional limitations. Occlusal appliances (OA) are among the therapies of choice. Methods. A single-blind randomized study was carried out to compare the therapeutic success of two different types of splints in patients with ADDWoR. A total of 30 subjects were eligible for the study out of the 330 screened. Group I (n = 15) received RA.DI.CA splint therapy and Group II (n = 15) received stabilization splint therapy. Temporomandibular pain, headache, neck pain and functional excursions were evaluated at baseline (T0), after 4 weeks (T1) and after 6 months (T2). Descriptive and inferential statistics were performed. Results. There was a significant increase in maximum jaw opening and a reduction in pain in both groups (p < 0.05), except for neck pain in Group II. Significant differences in between- and within-subject factors emerged in all of the parameters evaluated, especially between T1 and T2 scores, with a greater trend of improvement in Group I than Group II. Conclusion. RA.DI.CA splints were found to be more effective for the considered sample, especially in the treatment of comorbidities and functional movements, probably due to the greater orthopedic action and joint mobilization.
Stage 2: The Vaginal Flora in Women Undergoing Fetal Spina Bifida Repair and Its Potential Association with Preterm Rupture of Membranes and Preterm Birth
Authors: Tevaearai Fanny , Sachs Maike Katja , El-Hadad Samia , Vonzun Ladina , Moehrlen Ueli , Mazzone Luca , Meuli Martin , Krähenmann Franziska , Ochsenbein-Kölble Nicole
Published: November 2022

Introduction: Vaginal dysbiosis affects pregnancy outcomes, however, the relevance of abnormal findings on pre/post-surgical vaginal culture in women undergoing fetal spina bifida (fSB) repair is unknown. Objectives: To describe the incidence of normal and abnormal pre- and post-surgical vaginal microorganisms in fSB patients and to investigate potential associations between the type of vaginal flora and the occurrence of preterm prelabour rupture of membranes (PPROM) and preterm birth (PTB). Methods: 99 women undergoing fSB repair were eligible (2010–2019). Pre-surgical vaginal culture was routinely taken before surgery. Post-surgical cultures were taken on indication. Vaginal flora was categorized into four categories: healthy vaginal flora (HVF), bacterial vaginosis (BV), desquamative inflammatory vaginitis (DIV), and yeast infection. Results: The incidence of HVF, BV, DIV, or yeast infections was not statistically different between the pre- and postoperative patients. Furthermore, an abnormal pre/post-surgical vaginal flora was not associated with PPROM (OR 1.57 (0.74–3.32), p = 0.213)/OR 1.26 (0.62–2.55), p = 0.515), or with PTB (OR 1.19 (0.82–1.73), p = 0.315)/(OR 0.86 (0.60–1.24), p = 0.425). Conclusions: Abnormal vaginal microbiome was not associated with PPROM and PTB when appropriate treatment was performed.
Evaluation of a Liquid Media MALDI-TOF MS Protocol for the Identification of Dermatophytes Isolated from Tinea capitis Infections
Authors: Lecerf Pauline , De Paepe Roelke De , Jazaeri Yasaman , Normand Anne-Cécile , Martiny Delphine , Packeu Ann
Published: November 2022

One of the most common types of tinea is the superficial infection of the hair and scalp area known as tinea capitis. It is responsible for frequent outbreaks in nurseries and schools and represents a global health problem. Correct identification of the infection agent is essential in the determination of the infection source, epidemiological course, and treatment initiation. The conventional identification methods (direct exam, culture, DNA sequencing) are time-consuming, require experienced staff, are time-consuming, and the latter is expensive for routine identifications. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is gaining new ground for routine identification of filamentous fungi. The main advantages of MALDI-TOF MS are its rapid and accurate identification capability, relatively low cost, and easy integration into the laboratory routine. Its accuracy heavily depends on the quality of the reference spectra database. Identification of clinical isolates with MALDI-TOF MS protocol requires a sub-culturing step to ensure reliable identification. It can take days to weeks before fungal growth appears on solid medium. In this study, a unique MALDI-TOF MS protocol using liquid cultures of dermatophyte species was developed in order to shorten the turnaround time for the culture and identification of clinical isolates. Material and Method A standard MALDI-TOF MS protocol was adapted for liquid instead of solid cultures. Three different databases were tested. Results Using the liquid media MALDI-TOF MS protocol, a global rate of 62% correct identification (RCI) was obtained, compared with 87% for the protocol based on solid cultures. Trichophyton tonsurans was not correctly identified in all isolates using liquid cultures, with 88% of the isolates misidentified as Trichophyton interdigitale. The turnaround time for primary isolates for the solid and liquid protocols were respectively 11.7 and 11.6 days (no significant difference between both methods (p = 0.96)). Conclusions The newly designed liquid MALDI-TOF MS protocol did not lead to a significantly shorter turnaround time for the identification of dermatophytes isolated from tinea capitis infections. The turnaround time for the method with primary isolates was not significantly lower, and the rate of correct identification decreased remarkably, which emphasizes the need for a sub-culturing step. Using different database did not lead to improvement in turnaround time or rate of correct identification. This study highlights the importance of the medium and the reference database when performing MALDI-TOF MS.
Direct-Acting Antivirals and the Risk of Hepatitis B Reactivation in Hepatitis B and C Co-Infected Patients: A Systematic Review and Meta-Analysis
Authors: Oh Joo Hyun , Park Dong Ah , Ko Min Jung , Yoo Jeong-Ju , Yim Sun Young , Ahn Ji-Hyun , Jun Dae Won , Ahn Sang Bong
Published: November 2022

Hepatitis B (HBV) reactivation was observed to be more than 10% in patients receiving interferon-based therapy for hepatitis C (HCV) co-infection. At present, when direct-acting antiviral (DAA) has become the main treatment for HCV, there are few large-scale studies on the reactivation of HBV in these population. We studied HBV reactivation risk and prophylactic HBV treatment efficacy in HBV/HCV co-infected patients receiving DAA therapy. Relevant studies were selected from the Ovid-Medline, Ovid-EMBASE, Cochrane Central Register of Controlled Trials, KoreaMed, KMbase, and RISS databases through 4 September 2020. Data pooling was carried out using the random-effects method. We identified 39 articles with 119,484 patients with chronic (n = 1673) or resolved (n = 13,497) HBV infection under DAA therapy. When the studies were pooled, the HBV reactivation rate was 12% (95% confidence interval (CI) 6–19, I2 = 87%), indicating that this population needs careful attention. When stratified by baseline HBV DNA, the undetectable HBV DNA group showed a significantly lower risk of reactivation than the detectable HBV DNA group (odds ratio (OR) 0.30, 95% CI 0.11–0.86, I2 = 0%). Prophylactic HBV therapy reduced HBV reactivation risk (OR 0.25, 95% CI 0.07–0.92, I2 = 0%). Patients with a resolved HBV infection showed a negligible rate (0.4%) of HBV reactivation. In conclusion, patients with detectable HBV DNA levels warrant careful monitoring for HBV reactivation and may benefit from preventive anti-HBV treatment.
Quality of Life after Surgical Treatment for Chronic Otitis Media: A Systematic Review of the Literature
Authors: Lucidi Daniela , Cantaffa Carla , Nocini Riccardo , Martone Andrea , Alicandri-Ciufelli Matteo , Marchioni Daniele , Presutti Livio , Molinari Giulia
Published: November 2022

This systematic review aims to (a) define what instruments are available to measure quality of life (QoL) in patients undergoing tympanoplasty for chronic otitis media (COM) and what is the most commonly selected timing to do so; (b) compare outcomes from different surgical techniques; and (c) describe any reported correlation between subjective and functional results. This review was conducted following the PRISMA statement recommendations. Of the 151 articles screened, 24 were included. Most studies had a prospective design. The mean age at surgery was 44.5 years. A microscopic retroauricular approach was the most common surgical technique. Most articles included both primary and revision surgeries. The most commonly used questionnaire was the Glasgow Benefit Inventory (GBI), followed by the Chronic Ear Survey (CES), the Chronic Otitis Media Outcome Test 15 (COMOT-15) and the Zurich Chronic Middle Ear Inventory (ZCMEI-21). Questionnaires were administered about 12 months after surgery in most studies. Ten studies reported possible associations between hearing results and QoL. QoL assessment after COM surgery variably relies on disease-specific and non-specific questionnaires. Patients are usually evaluated 12 months after surgery, and this appears to be a suitable timing to contrast the possible bias effect of different tympanoplasty techniques associated with different healing times. A comparison between QoL outcomes in different surgical approaches cannot be made, as several influencing factors have not been detailed in the included studies. Few studies have investigated the correlation between subjective and objective outcomes of tympanoplasty for COM so far.
Use of High-Frequency Transducers in Breast Sonography
Authors: Corvino Antonio , Varelli Carlo , Catalano Fabio , Cocco Giulio , Delli Pizzi Andrea , Boccatonda Andrea , Corvino Fabio , Basile Luigi , Catalano Orlando
Published: November 2022

Sonography companies have recently developed high-frequency transducers (20–30 MHz) to image the skin and small joints. In this pictorial review, we present a number of settings where these probes can be usefully employed to scan the breast. These include skin abnormalities of the breast and axilla; nipple–areolar complex abnormalities; superficial breast parenchyma abnormalities; breast parenchyma abnormalities in subjects with implants; very small female breasts; peripheral areas in breasts of any size; pre-puberal breasts; male breasts; post-mastectomy chest wall; and intraoperative breast sonography. Comparatively, side-by-side images obtained with conventional breast frequencies and high frequencies are shown.
Enhanced Permeability and Retention Effect as a Ubiquitous and Epoch-Making Phenomenon for the Selective Drug Targeting of Solid Tumors
Authors: Islam Waliul , Niidome Takuro , Sawa Tomohiro
Published: November 2022

In 1979, development of the first polymer drug SMANCS [styrene-co-maleic acid (SMA) copolymer conjugated to neocarzinostatin (NCS)] by Maeda and colleagues was a breakthrough in the cancer field. When SMANCS was administered to mice, drug accumulation in tumors was markedly increased compared with accumulation of the parental drug NCS. This momentous result led to discovery of the enhanced permeability and retention effect (EPR effect) in 1986. Later, the EPR effect became known worldwide, especially in nanomedicine, and is still believed to be a universal mechanism for tumor-selective accumulation of nanomedicines. Some research groups recently characterized the EPR effect as a controversial concept and stated that it has not been fully demonstrated in clinical settings, but this erroneous belief is due to non-standard drug design and use of inappropriate tumor models in investigations. Many research groups recently provided solid evidence of the EPR effect in human cancers (e.g., renal and breast), with significant diversity and heterogeneity in various patients. In this review, we focus on the dynamics of the EPR effect and restoring tumor blood flow by using EPR effect enhancers. We also discuss new applications of EPR-based nanomedicine in boron neutron capture therapy and photodynamic therapy for solid tumors.
Potential Association of Isolated γ-Glutamyltransferase Elevation with Incident Ischemic Heart Disease in Lean Koreans
Authors: Sung Yumin , Lee Yong-Jae , Jung Dong-Hyuk , Park Byoungjin
Published: November 2022

Isolated elevation of γ-glutamyltransferase (GGT), a microsomal membrane-bound protein, is commonly observed in non-obese Koreans without diabetes, and its clinical implications are not well-known. Therefore, we aimed to investigate the longitudinal effect of isolated GGT on the incidence of ischemic heart disease (IHD) risk in a large cohort of lean non-diabetic Koreans. Data were obtained from the Health Risk Assessment Study (HERAS) and Korea Health Insurance Review and Assessment (HIRA) datasets. The participants were divided into four groups according to the GGT quartile after the exclusion of those participants with diabetes, a body mass index (BMI) ≥ 25 kg/m2, alanine aminotransferase (ALT) ≥ 40 IU/L, and aspartate aminotransferase (AST)/ALT > 1.5, as well as those positive for hepatitis B surface antigen or hepatitis C antibody. We prospectively assessed the hazard ratios (HRs) with 95% confidence intervals (CIs) for IHD using multivariate Cox proportional hazard regression models over a 50-month period. During the follow-up period, 183 individuals (1.85%) developed IHD. After setting the lowest GGT quartile as a reference group, the HRs of IHD for GGT quartiles 2–4 were 1.66 (95% CI 0.95–2.89), 1.82 (95% CI 1.05–3.16), and 1.98 (95% CI 1.12–3.50), respectively, after adjusting for age, sex, body mass index, smoking status, alcohol consumption, physical activity, mean arterial blood pressure, fasting plasma glucose, and dyslipidemia. An isolated high GGT may be an additional measure for assessing and managing future IHD risks among lean Koreans without diabetes.
Body Motion Sensor Analysis of Human-Induced Dynamic Load Factor (DLF) for Normal Walks on Slender Transparent Floors
Authors: Bedon Chiara , Fasan Marco , Noè Salvatore
Published: November 2022

Modern constructions are often characterized by the presence of slender and aesthetically fascinating components and assemblies. For pedestrian systems in particular, such constructions are notoriously associated with possible vibration issues, and thus require special calculations. When these slender systems are made of structural glass, additional effects due to transparency may also affect human behaviours and motions. In this paper, based on a single body motion, a microelectromechanical system (MEMS) sensor in the body’s centre of mass (CoM) is introduced, an extended, original experimental investigation is presented, and human-induced effects on slender transparent floors are discussed. Major attention is given to the well-known dynamic load factor (DLF) induced by a single pedestrian’s normal walk; a fixed walking rate is assigned, and different substructures (with major variations in their structural dynamic parameters) are taken into account. A discussion of experimental results is proposed for rigid reinforced concrete (RC), and a laboratory contrast system (SLAB#1), which is used as a reference for the analysis of DLF trends on relatively light and flexible transparent glass flooring systems (SLAB#2 and SLAB#3). It is shown that structural frequency and mass, but also possibly transparency, can affect human motion and result in a quantitative modification of measured DLF values, especially for the first and second harmonics of vertical force components.
Impact of Transhumant Livestock Grazing Abandonment on Pseudo-Alpine Grasslands in Greece in the Context of Climatic Change
Authors: Sidiropoulou Anna , Chouvardas Dimitrios , Mantzanas Konstantinos , Stefanidis Stefanos , Karatassiou Maria
Published: November 2022

Pseudo-alpine grassland ecosystems have started to decline during the past few decades. According to many studies, climate change and abandonment of traditional anthropogenic activities are directly linked to this phenomenon. However, the interaction of these two factors with pseudo-alpine grasslands has not been studied in Greece. The aim of this study was to assess the impact of climatic change and abandonment of transhumant livestock grazing on pseudo-alpine grassland ecosystems structure and stability in Mt Vermio and Mt Zireia. Geographic Information System data on land use/land cover from 1945 and 2020, as well as climatological and livestock data, have been examined and presented. Landscape metrics were also used to quantify landscape structure changes. Although both mountains’ pseudo-alpine grasslands have reduced in size, Mt Zireia has experienced an upward treeline shift, which seems to be the result of climate change, while in Mt Vermio, the more severe transhumance abandonment caused horizontal tree expansion. There are strong indications that a rise in temperature is the main driver for the upward increase in treeline.
Wood-Based Products in the Circular Bioeconomy: Status and Opportunities towards Environmental Sustainability
Authors: Hassegawa Mariana , Van Brusselen Jo , Cramm Mathias , Verkerk Pieter Johannes
Published: November 2022

The circular bioeconomy offers solutions to curb the effects of climate change by focusing on the use of renewable, biological resources to produce food, energy, materials, and services. The substitution of fossil products by wood-based products can help avoid or reduce greenhouse gas emissions over the life cycle of products. However, it is important to understand the potential impacts of large-scale material substitution at the market level. This study aimed to assess the role of selected wood-based products in the circular bioeconomy, the possible changes in their markets, and investigate which elements could ensure the environmental sustainability of these products. The demand for graphic paper has declined over the last 15 years, while the demand for packaging has increased. Cross-laminated timber and man-made cellulosic fibres have seen their global consumption increase over the last decade. While there are benefits associated with the substitution of non-renewable materials by wood-based products, there is still limited understanding of the substitution effects at market-, country- and global level. Some factors enabling the further uptake of wood-based products include initiatives that stimulate technological change, incentives to produce or consume less fossil-based and more bio-based alternatives, and the promotion and marketing of wood-based products as viable alternatives to non-renewable materials.
Testing the Effects of Water-Saving Technologies Adapted to Drought: Empirical Evidence from the Huang-Huai-Hai Region in China
Authors: Song Chunxiao , Rong Yue , Liu Ruifeng , Oxley Les , Ma Hengyun
Published: November 2022

The aggravation of extreme weather events has dramatically increased the risk of severe water shortages and seriously threatened agricultural production. The Huang-Huai-Hai region, an important agricultural production region in China, is subject to a severe water shortage and is often hit by drought. As a result, water-saving technologies (WSTs) have been implemented. It remains unclear how effectively these WSTs can reduce crop yield loss, crop yield variation, and the loss of net crop income caused by water scarcity. Therefore, this paper aimed to analyze the role of WSTs in response to drought by establishing a multi-objective expected utility function based on 988 farmers across the Huang-Huai-Hai region. Econometric analysis employing an endogenous switching regression model showed that using WSTs can significantly reduce crop yield loss and net income loss caused by drought. Adopting household-based WSTs or community-based water-saving technology generates even greater positive effects on crop yield and farmers’ net income. Therefore, the government should promote farmers’ adoption of more advanced WSTs by increasing subsidies and strengthening policy support.
Evaluation of Urban Thermal Comfort and Its Relationship with Land Use/Land Cover Change: A Case Study of Three Urban Agglomerations, China
Authors: Shu Yuqing , Zou Kang , Li Guie , Yan Qingwu , Zhang Siyu , Zhang Wenhao , Liang Yuqing , Xu Wenzhou
Published: November 2022

With the acceleration of urbanization in China, the urban surface thermal environment has undergone significant changes. This work aims to calculate the urban thermal comfort index using a temperature and humidity model with the land surface temperature and relative humidity. It also aims to explain the association between the land use/land cover change (LUCC) and urban surface thermal environment of the Beijing–Tianjin–Hebei (BTH) Region, the Guangdong–Hong Kong-Macao Greater Bay Area (GBA) and the Yangtze River Delta (YZD) in 2020, 2015, 2010 and 2005 using geographically weighted regression. The results reveal that (1) the three urban agglomerations have substantial heat island intensity regions, which are clustered and zonally distributed, and the annual average rates of the heat island area growth in the three regions are 1.01%, 1.41% and 1.09%, respectively. (2) Many uncomfortable areas exist in the three urban agglomerations, with an exponential growth trend in summer, and the annual average proportion of the uncomfortable areas in the three regions are 60.8%, 56.8% and 49.4%, respectively. (3) From the spatial point of view, the high-thermal comfort index areas of Beijing, Tianjin, Hebei, Guangdong, Hong Kong and Macao expand to the coast, and the high-index areas of the Yangtze River Delta expand to the inland. In terms of time, the annual distribution of thermal comfort in Beijing–Tianjin–Hebei is discrete, but the annual distribution of thermal comfort in Guangdong, Hong Kong, Macao and the Yangtze River Delta is concentrated. (4) In LUCC, the change intensity in construction land has a remarkable effect on the change in thermal comfort. The areas where the thermal comfort index increases positively correlate with the areas where the construction land increases. This study enriches the research on the impact of LUCC on urban ecological performance, and thus provides the necessary scientific basis for urban environment construction.
Small Farms in Italy: What Is Their Impact on the Sustainability of Rural Areas?
Authors: Cardillo Concetta , Cimino Orlando
Published: November 2022

Small farms are gaining space and importance within the agricultural policies implemented by the European Union, mainly for the role that they play for the preservation of the territory and for the economic development of local rural areas. Small farms represent a new opportunity to guarantee the permanence of populations and agricultural workers in rural areas, contributing to the formation of the income of farming families. Therefore, in this study, after identifying small farms as those farms that have a Standard Output (SO) of less than EUR 25,000, their structural characteristics were defined, as well as their economic and financial situation. The analysis was performed using the Italian FADN data for the years 2018–2020 and using a set of structural and economic–financial indicators. Furthermore, the study analyzes the relationship between farm performance and agricultural resources and also with farmer demographics and farm size. The principal Component analysis was used to reduce the number of variables used in the Ordinary Least Square (OLS) regression model which was applied to identify the factors contributing to the small farms’ profitability. The territorial distribution of small farms shows a polarization: 37% of them are in Southern Italy, and more than 34% of them are in Northern Italy. The analysis also reveals that about 67% of the Italian small farms are specialized, in particular, in arable land (37.6%) and herbivores (16.8%). They are mainly conducted by men with a high school education level and with an age that is between 40 and 65 years. The economic results also show a good performance, however, there is a wide district differentiation: while the Northern regions have the best results in terms of farm net income, those of Southern Italy are more dependent on the public support they receive. The results of the multiple linear regression analysis revealed which variables (e.g., land size, labor, public aid, etc.) had a direct relationship with the profitability of small farms. The research provides interesting insights to stakeholders on the public support (specific measures) that needs to be designed and implemented to favor the survival of small farms in rural areas.
The Ecological and Social Effects of Cropland Expansion in the HehuangValley during theMing and Qing Dynasties
Authors: Hou Zhirui , Lancuo Zhuoma , Hou Guangliang
Published: November 2022

In this paper, we strive to showthat the protection of the ecological environment of the Yellow River can impact regional sustainable development and human society. Based on GIS and historical documents, we selected 1640AD in the late Ming Dynasty and 1726, 1746, and 1856AD in the early and middle Qing Dynasty as time sections to reconstruct the distributions of cropland and vegetation in the Hehuang valley. Our results showed that the cropland in the Ming Dynasty was mainly distributed in the valley of Sainei;during the early and mid-Qing Dynasty, the cropland reclamation broke the boundary of the Great Wall. Furthermore, replacing vegetation with cropland resulted in the rapid decline of water conservation capacity in the medium and high mountain areas. The decline of water conservation capacity significantly contributed to the frequent occurrence of natural disasters, such as drought, flood, water erosion, and sand pressure, which led to decreased cropland output. By the mid-Qing Dynasty, the cropland area had saturated while the population was still growing, and the grain yield could not meet the demands of the expanding population. Due to both natural and social factors, two social upheavals occurred in the late Qing Dynasty, which significantly affected the development of the regional social economy. Therefore, the destruction of the ecological environment and the reduction of water conservation capacity became an important driving force for the destruction of sustainable regional development.
Urban Layout of the First Ibero-American Cities on the Continent through Conventual Foundations: The Cases of Santo Domingo (1502) and Panama Viejo (1519)
Authors: Cubero-Hernández Antonio , Raony Silva Eudes , Arroyo Duarte Silvia
Published: November 2022

In the present investigation, we study the influence of conventual foundations on the origin of the urban layout of two of the first cities in the Spanish colonization of America: Santo Domingo (1502) and Panama Viejo (1519), examples of early colonial urbanism. Starting with cartography and historical bibliography, as well as recent studies on their urban evolution and using Geographic Information Systems (GIS) for graphic representation, a comparative study is carried out between both cities divided into stages of urban expansion until their consolidation (or disappearance in the case of Panama in 1671). We have been able to verify how, in Santo Domingo, the religious orders settled on the outskirts of the city, marking the axes of expansion as an instrument of control and consolidation of an urban layout closer to the idea of a grid, which will materialize more precisely in later cities. Meanwhile, in Panama Viejo, the city was formed longitudinally on the streets occupied by the convents, which served as the main axes that defined the urban design of the city. This article aims to demonstrate the importance of the role of religious power in the formation of the cities presented here.
Community Structure and Distribution Pattern of Woody Vegetation in Response to Soil properties in Semi-Arid Lowland District Kasur Punjab, Pakistan
Authors: Waheed Muhammad , Arshad Fahim , Majeed Muhammad , Fatima Sammer , Mukhtar Naila , Aziz Robina , Mangrio Wali Muhammad , Almohamad Hussein , Al Dughairi Ahmed Abdullah , Al-Mutiry Motrih , Abdo Hazem Ghassan
Published: November 2022

Plant diversity is lower in arid regions around the world. However, semi-arid regions have very high species richness and are notable in terms of vegetation structure and plant diversity. The major goal of this study was to assess the composition and diversity of woody species with respect to edaphic properties in semi-arid lowlands of Kasur, Punjab Pakistan. Comprehensive field surveys were conducted to study the botanical diversity of woody vegetation in Kasur district (Punjab) during 2020–2022. Based on geography and vegetation richness, 120 sampling sites were selected, with each site comprising 05 transects of 500 m2 randomly. Therefore, from each sampling site, 25 quadrats were taken for exploring the biological assortment of woody vegetation. Soil samples were collected at a depth of 9 to 12 cm and placed in a polythene bag. The soil samples were tested for soil pH, EC, OM, macronutrients (N, P, and K), and cation exchange capacity (CEC). A total of 86 woody species belonging to 61 genera and 26 families were documented from diverse habitats of semi-arid lowland of Kasur, Punjab, Pakistan. The leading family was Leguminosae having 13 genera and 21 species, followed by Moraceae, including four genera and nine species, and Bignoniaceae with seven genera and 09 species. Overall, five woody plant communities were renamed by means of the maximum indictor-valued plant species, such as those listed below; 1. EDM: Eucalyptus-Dalbergia-Morus community; 2. PBM: Populus-Bombax-Morus community; 3. ZTZ: Ziziphus-Tamarix- Ziziphus community; 4. PAP: Prosopis-Acacia-Prosopis community, and 5. BCR: Broussonetia-Conocarpus-Ricinus community. The CCA ordination makes it apparent that appropriate edaphic aspects, such as pH, organic matter, N, P, K, soil texture (silt, sand, and clay), and cation exchange capacity (CEC), have a significant influence on how woody species are distributed. The current effort provides a fundamental layout to realize the influences of environmental variables on the arrangement, variety, and relations of woody vegetation, which is useful to improve the conservation and management events for the ecological restoration of degraded habitation in the studied district.
Cation Exchange Resins for Predicting Available K on K-Deficient Soils: Extraction Capacity among Different Soil K Pools and First Insights on the Contribution of K Solubilized by Rhizosphere Microbes
Authors: Bilias Fotis , Kotsangeli Eleni , Ipsilantis Ioannis , Barbayiannis Nikolaos
Published: November 2022

The contribution of non-exchangeable soil K in the total K availability may be essential to K deficient soils; however, less attention has been paid so far for proposing soil tests that assess the bioavailable structural K that is solubilized by soil microbes during a growing season. The objectives of this study were to evaluate cation exchange resins for their performance in predicting K uptake by plants; to investigate the relationships between rhizosphere soil microbes, different soil K pools, and soil parameters; and to assess their exchange capacity with respect to K derived by feldspars. A pot experiment with winter wheat was conducted on K deficient soils, several soil tests were examined, and rhizosphere K solubilizing microbial population was assessed. Single and multiple regressions showed that cation resins performed better in predicting total plant K uptake than the other chemical extractants (r2 = 0.64, r2 = 0.85, respectively, p ≤ 0.001), whereas the PCA analysis and Pearson correlation tests revealed a positive correlation between K derived by feldspars, K uptake, and the K solubilizing rhizosphere microbial population. The above was further confirmed by the mapping of cation resins of extraction capacity, which showed a significant contribution of K derived by feldspars (15.6%).
Feasibility of Reflectance Confocal Microscopy Monitoring in Oily, Acne-Prone Facial Skin Treated with a Topical Combination of Alpha and Beta-Hydroxy Acids, Anti-Inflammatory Molecules, and Herculane Thermal Water: A Blinded, One-Month Study
Authors: Lupu Mihai , Malciu Ana Maria , Voiculescu Vlad Mihai
Published: November 2022

Oily, acne-prone skin is a common skin type which may be monitored in vivo using reflectance confocal microscopy (RCM). The aim of the study was to assess the feasibility of RCM in evaluating the effectiveness of a topical combination of alpha- and beta-hydroxy acids, anti-inflammatory and antibacterial molecules, and Herculane thermal water on acne-prone skin. Thirty-five subjects with oily, acne-prone skin were prescribed topical combination products and were evaluated by clinical, Wood’s lamp, and RCM imaging at baseline and after 28 days. At 28 days, the RCM-evaluated number of dilated infundibula, infundibula filled with keratotic material, and infundibula with thickened bright borders, as well as the density of the inflammatory infiltrate, were significantly decreased. Wood’s light images at 28 days showed a significantly reduced number of C. acnes-colonized infundibula, and both the median area and the intensity of the red-orange fluorescence were decreased. The reduction in the clinical score was concurrent with the improvement in the RCM parameters, suggesting that this non-invasive imaging technique is appropriate for efficiency evaluations of topical acne treatments.
Polycystic Ovary Syndrome: An Updated Overview Foregrounding Impacts of Ethnicities and Geographic Variations
Authors: Yasmin Afrin , Roychoudhury Shubhadeep , Paul Choudhury Arun , Ahmed A. B. Fuzayel , Dutta Sulagna , Mottola Filomena , Verma Vivek , Kalita Jogen C. , Kumar Dhruv , Sengupta Pallav , Kolesarova Adriana
Published: November 2022

Polycystic ovary syndrome (PCOS) is one of the most common heterogeneous conditions of the endocrine reproductive system in women of childbearing age. Hyperandrogenism and oligomenorrhea are the two core characteristics of PCOS, a complicated and multifaceted illness. The condition is also linked to several major side effects, which include type 2 diabetes, early atherosclerosis, infertility, and endometrial cancer. There are few facts and statistics available on PCOS prevalence internationally due to the significant degree of geographic and ethnic variance and inconsistency caused by different diagnosis standards. Limited (n = 179) explorations have been made in the context of the prevalence of this complicated illness so far, and out of these, only 55 studies have discussed its association with race and/or ethnicity. However, those studies remain restricted due to the small sample size, biased selection, and the lack of comparative studies. Variations in PCOS prevalence frequency also arise due to different diagnostic criteria, as well as racial and ethnic differences, associated lifestyle factors, and subsequent illnesses that affect the accuracy of the diagnosis. The main objective behind this systematic review is to provide comprehensive epidemiological data on PCOS that is organized geographically. This evidence-based study also provides an overview of the clinical management of PCOS to instigate further research on this complex endocrinological condition and the subsequent development of preventive treatment strategies.
A Retrospective Comparative Study of Sodium Fluoride Na18F-PET/CT and 68Ga-PSMA-11 PET/CT in the Bone Metastases of Prostate Cancer Using a Volumetric 3-D Radiomic Analysis
Authors: Kairemo Kalevi , Kangasmäki Aki , Kappadath Srinivasan , Joensuu Timo , Macapinlac Homer
Published: November 2022

Bone is the most common metastatic site in prostate cancer (PCa). 68Ga-PSMA-11 (or gozetotide) and sodium fluoride-18 (Na18F) are rather new radiopharmaceuticals for assessing PCa-associated bone metastases. Gozetotide uptake reflects cell membrane enzyme activity and the sodium fluoride uptake measures bone mineralization in advanced PCa. Here, we aim to characterize this difference and possibly provide a new method for patient selection in targeted therapies. Methods: The study consisted of 14 patients with advanced PCa (M group > 5 lesions), who had had routine PET/CT both with PSMA and NaF over consecutive days, and 12 PCa patients with no skeletal metastases (N). The bone regions in CT were used to coregister the two PET/CT scans. The whole skeleton volume(s) of interest (VOIs) were defined using the CT component of PET (HU > 150); similarly, the sclerotic/dense bone was defined as HU > 600. Additional VOIs were defined for PET, with pathological threshold values for PSMA (SUV > 3.0) and NaF (SUV > 10). Besides the pathological bone volumes measured with each technique (CT, NaF, and PSMA-PET) and their contemporaneous combinations, overlapping VOIs with the CT-based skeletal and sclerotic volumes were also recorded. Additionally, thresholds of 4.0, 6.0, and 10.0 were tested for SUVPSMA. Results: In group M, the skeletal VOI volumes were 8.77 ± 1.80 L, and the sclerotic bone volumes were 1.32 ± 0.50 L; in contrast, in group N, they were 8.73 ± 1.43 L (skeletal) and 1.23 ± 0.28 L (sclerosis). The total enzyme activity for PSMA was 2.21 ± 5.15 in the M group and 0.078 ± 0.053 in the N group (p < 0.0002). The total bone demineralization activity for NaF varied from 4.31 ± 6.17 in the M group and 0.24 ± 0.56 in group N (p < 0.0002). The pathological PSMA volume represented 0.44–132% of the sclerotic bone volume in group M and 0.55–2.3% in group N. The pathological NaF volume in those patients with multiple metastases represented 0.27–68% of the sclerotic bone volume, and in the control group, only 0.00–6.5% of the sclerotic bone volume (p < 0.0003). Conclusions: These results confirm our earlier findings that CT alone does not suit the evaluation of the extent of active skeletal metastases in PCa. PSMA and NaF images give complementary information about the extent of the active skeletal disease, which has a clinical impact and may change its management. The PSMA and NaF absolute volumes could be used for planning targeted therapies. A cut-off value 3.0 for SUVPSMA given here is the best correlation in the presentation of active metastatic skeletal disease.
Muscular Response in ALS Patients during Maximal Bilateral Isometric Work of the Biceps Brachii until Fatigue
Authors: Alarcón-Jimenez Jorge , de la Rubia Ortí Jose Enrique , Martín Ruiz Julio , de Bernardo Nieves , Proaño Belén , Villarón-Casales Carlos
Published: November 2022

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative and fatal disease, characterized by the loss of motor neurons and progressive musculoskeletal deterioration. The clinical onset is mainly bulbar or spinal. Considering that there is no effective medical treatment, there is a need to understand the muscle activation patterns to design better physical exercise routines. The objective of this study was to determine muscle strength and fatigue in patients with ALS performing a unilateral exercise, and according to sex and type of ALS. A cross-sectional, analytical study was conducted with 23 patients. Five maximal unilateral isometric contractions were performed with the right and left biceps brachii. Muscle activation was calculated by surface electromyography bilaterally in the biceps brachii, triceps brachii, rectus femoris anterior, and tibialis anterior. The results showed more accentuated fatigue in men than in women, between the first and last contractions performed and especially on the dominant side (p = 0.016). In addition, there was evidence of a coactivation effect on the muscles around the work joint, which reflects a growing activation of synergists, regardless of sex or type of ALS. These findings support the use of systematic and extensive resistance exercise as a non-invasive option for maintaining the functional capacity of patients with ALS.
Analysis of Rac/Rop Small GTPase Family Expression in Santalum album L. and Their Potential Roles in Drought Stress and Hormone Treatments
Authors: Chen Yu , Wang Shengkun , Liu Xiaojing , Wang Dongli , Liu Yunshan , Hu Lipan , Meng Sen
Published: November 2022

Plant-specific Rac/Rop small GTPases, also known as Rop, belong to the Rho subfamily. Rac proteins can be divided into two types according to their C-terminal motifs: Type I Rac proteins have a typical CaaL motif at the C-terminal, whereas type II Rac proteins lack this motif but retain a cysteine-containing element for membrane anchoring. The Rac gene family participates in diverse signal transduction events, cytoskeleton morphogenesis, reactive oxygen species (ROS) production and hormone responses in plants as molecular switches. S. album is a popular semiparasitic plant that absorbs nutrients from the host plant through the haustoria to meet its own growth and development needs. Because the whole plant has a high use value, due to the high production value of its perfume oils, it is known as the “tree of gold”. Based on the full-length transcriptome data of S. album, nine Rac gene members were named SaRac1-9, and we analyzed their physicochemical properties. Evolutionary analysis showed that SaRac1-7, AtRac1-6, AtRac9 and AtRac11 and OsRac5, OsRacB and OsRacD belong to the typical plant type I Rac/Rop protein, while SaRac8-9, AtRac7, AtRac8, AtRac10 and OsRac1-4 belong to the type II Rac/ROP protein. Tissue-specific expression analysis showed that nine genes were expressed in roots, stems, leaves and haustoria, and SaRac7/8/9 expression in stems, haustoria and roots was significantly higher than that in leaves. The expression levels of SaRac1, SaRac4 and SaRac6 in stems were very low, and the expression levels of SaRac2 and SaRac5 in roots and SaRac2/3/7 in haustoria were very high, which indicated that these genes were closely related to the formation of S. album haustoria. To further analyze the function of SaRac, nine Rac genes in sandalwood were subjected to drought stress and hormone treatments. These results establish a preliminary foundation for the regulation of growth and development in S. album by SaRac.