bluuebunny/crossref_metadata_2025 huggingface dataset:
Framework Construction and Dynamic Characteristics of Spring Low-Temperature Disasters Affecting Winter Wheat in the Huang-Huai-Hai Region, China
Authors: Li Meixuan , Huo Zhiguo , Mi Qianchuan , Zhang Lei , Wang Yi , Kong Rui , Jiang Mengyuan , Zhang Fengyin
Published: December 2024

The accurate and sub-daily identification of agricultural low-temperature disasters (LTDs) facilitates the understanding of their dynamic evolution, the evaluation of the characteristics of disaster events, and informs effective strategies aimed at disaster prevention and mitigation. In order to ensure the timely, precise, and comprehensive capture of disaster processes, we have developed a dynamic evaluation framework for winter wheat spring LTD in the Huang-Huai-Hai (HHH) region, driven by meteorological data. This framework consists of two primary components: a disaster classification module and a dynamic simulation-assessment module. Through disaster mechanisms and comprehensive statistical analysis, we have established the input features and structural framework of the classification module using a decision tree algorithm. The dynamic simulation evaluation module is based on our newly developed index for the cumulative hourly intensity of low-temperature stress (CHI) and its grade indicators. This index integrates the interaction between cold stress (low-temperature intensity, cooling amplitude, and duration) and mitigating conditions (air humidity) during the evolution process of LTD. Based on CHI, we found that as the intensity of low temperatures and the amplitude of cooling rise, along with an extended duration of stress and a reduction in relative humidity, the severity of spring LTDs in winter wheat get worse. The overall validation accuracy of the evaluation framework is 92.6%. High validation accuracy indicates that our newly established framework demonstrates significant efficacy in identifying LTDs and assessing grade. Through the analysis of the characteristics of the disaster process, spring LTDs affecting winter wheat are mainly mild, with frost identified as the primary category of LTD. The duration of freeze injury typically exceeds 24 h, while the duration of frost damage and cold damage is less than 24 h. From 1980 to 2022 in the HHH region, the frequency of spring freeze injury and frost damage on winter wheat showed an overall decreasing trend, with a particularly significant decrease in frost damage occurrences. Conversely, cold damage occurrences are on the rise. In addition, the duration of individual disaster events for the three categories of spring LTDs is decreasing, while both the average intensity and extremity of these events show increasing trends. This study has important practical value for the sub-daily scale evaluation of the spring LTD affecting winter wheat in the HHH region and serves as an effective guide for agricultural disaster prevention and mitigation, as well as for the formulation of planting strategies.
Boron Nitride Nanostructures (BNNs) Within Metal–Organic Frameworks (MOFs): Electrochemical Platform for Hydrogen Sensing and Storage
Authors: Alamro Azizah , Balbaied Thanih
Published: December 2024

Boron nitride nanostructures (BNNs), including nanotubes, nanosheets, and nanoribbons, are renowned for their exceptional thermal stability, chemical inertness, mechanical strength, and high surface area, making them suitable for advanced material applications. Metal–organic frameworks (MOFs), characterized by their porous crystalline structures, high surface area, and tunable porosity, have emerged as excellent candidates for gas adsorption and storage applications, particularly in the context of hydrogen. This paper explores the synthesis and properties of BNNs and MOFs, alongside the innovative approach of integrating BNNs within MOFs to create composite materials with synergistic properties. The integration of BNNs into MOFs enhances the overall thermal and chemical stability of the composite while improving hydrogen sensing and storage performance. Various synthesis methods for both BNNs and MOFs are discussed, including chemical vapor deposition, solvothermal synthesis, and in situ growth, with a focus on their scalability and reproducibility. Furthermore, the mechanisms underlying hydrogen sensing and storage are examined, including physisorption, chemisorption, charge transfer, and work function modulation. Electrochemical characterization techniques, such as cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge–discharge, are used to analyze the performance of BNN-MOF systems in hydrogen storage and sensing applications. These methods offer insights into the material’s electrochemical behavior and its potential to store hydrogen efficiently. Potential industrial applications of BNN-MOF composites are highlighted, particularly in fuel cells, hydrogen-powered vehicles, safety monitoring in hydrogen production and distribution networks, and energy storage devices. The integration of these materials can contribute significantly to the development of more efficient hydrogen energy systems. Finally, this study outlines key recommendations for future research, which include optimizing synthesis techniques, improving the hydrogen interaction mechanisms, enhancing the stability and durability of BNN-MOF composites, and performing comprehensive economic and environmental assessments. BNN-MOF composites represent a promising direction in the advancement of hydrogen sensing and storage technologies, offering significant potential to support the transition toward sustainable energy systems and hydrogen-based economies.
Comparative Study of Gut Microbiome in Urban and Rural Eurasian Tree Sparrows
Authors: Yan Shuai , Zhang Yu , Huang Ji , Liu Yingbao , Li Shaobin
Published: December 2024

Gut microbiota play a significant role in various physiological functions, including digestion, nutritional metabolism, and host immune function. The composition of these gut microbes is largely influenced by habitats. This study examines the gut microbiota of the Eurasian tree sparrow (Passer montanus) inhabiting rural and urban environments to understand the effects of habitat variation on microbial composition. We captured 36 rural and 29 urban adult tree sparrows and observed minor differences in body mass but substantial differences in foraging microhabitats between the two groups. Fecal samples from adult males with similar body mass were selected for a gut microbiome analysis to mitigate potential confounding effects, resulting in 20 successfully sequenced samples. The analysis disclosed disparities in gut microbiota diversity and composition between rural and urban sparrows. The urban group demonstrated slightly higher alpha diversity and distinct dominant phyla and genera compared to the rural group. Additionally, differences in the relative abundance of potentially pathogenic bacteria were observed between the groups. Several potentially pathogenic bacteria (e.g., TM7, Staphylococcus, Helicobacter, and Shigella) were more abundant in the urban group, suggesting that tree sparrows may act as transmission vectors and develop stronger immune systems. This could potentially facilitate pathogen dissemination while also contributing to the natural cycling of nutrients and maintaining ecosystem health in urban environments. The beta diversity analysis confirmed structural differences in microbial communities, implicating habitat variation as a contributing factor. Furthermore, the LEfSe analysis emphasized significant differences in gut bacteria abundance (across two phyla, three classes, six orders, seven families, and eight genera) between urban and rural sparrows, with predicted functional differences in metabolic pathways. Notably, lipid metabolism was enriched in urban sparrows, indicating enhanced lipid synthesis and metabolism in urban habitats. In conclusion, this study underscores the profound influence of habitat on the gut microbiota composition and functional potential in tree sparrows. Our findings highlight that urbanization alters the gut microbes and, consequently, the physiological functions of bird species.
Is It Really a Failure? A Survey About Foster Animal Adoption
Authors: Reese Laura A.
Published: December 2024

The widespread use of the term “foster fail” in animal rescue suggests that it happens often, but no research has explored the prevalence of volunteers adopting their foster animals or whether the phenomenon is really a “failure”. This survey-based study focused on the following questions: 1. How common are foster fails among volunteers on shelter and rescue lists and why do they occur? 2. What types of volunteers are most likely to adopt their foster animals? 3. Do different attachment styles to pets affect foster adoption? 4. Is the adoption of foster animals a way to deal with the potential grief of letting them go to adoption? 5. What are the impacts of foster fails on animal shelters in terms of longevity of volunteers and satisfaction with the volunteer experience? Data were collected through surveys of foster volunteers. Two nonprofit organizations, the Pedigree Foundation and Shelter Animals Count, distributed information about the survey and shelter directors distributed the survey link to their population of foster volunteers. Nine hundred and forty-seven individuals responded. To address the research questions, frequency, correlation, and regression analyses were employed. A total of 38% of volunteers had not adopted a foster in the past ten years, and another 38% had adopted one or two; 90 (11%) and 103 (13%) had adopted three to four or more than four, respectively. Volunteers that had significantly higher numbers of foster fails were those that were older (r = 0.22, p < 0.001), retired (chi-squared = 9.05, p = 0.029), lower on educational attainment (r = −0.13, p < 0.001), female with their own cats (r = 0.16, p < 0.001), and part of a fostering family (r = 0.08, p = 0.043). Volunteers that expressed higher levels of both people-substituting (r = 0.16, p = 0.003) and general (r = 0.13, p = 0.017) attachment to their fosters were more likely to adopt them, as were those that more frequently fostered animals with special medical or behavioral needs (r = 0.25, p < 0.001). Volunteers that had longer tenures (r = 0.43, p < 0.001), fostered more frequently (r = 0.24, p < 0.001), and reported greater resilience (r = 0.10, p = 0.009) had adopted significantly more animals. Finally, there was a significant and positive relationship between satisfaction with fostering and adopting more foster animals (r = 0.16, p < 0.001). The findings indicated that instead of being a “failure,” foster adoptions can be a positive force for the animal in question, their adopters, and shelters and rescues because they have more resilient, satisfied, and active volunteers.
Bovine Respiratory Disease in Veal Calves: Benefits Associated with Its Early Detection by Lung Ultrasonography and Its Prompt Treatment with a Single Dose of a Fixed Combination of Florfenicol and Meloxicam
Authors: Lisuzzo Anastasia , Achard Damien , Valenza Alessio , Contiero Barbara , Cozza Luca , Schiavon Eliana , Catarin Giacomo , Conte Fabio , Fiore Enrico
Published: December 2024

Lung ultrasonography can facilitate bovine respiratory disease (BRD) detection and can potentially improve treatment outcomes. In this study, ninety-six veal calves were followed weekly with clinical and lung ultrasound examinations during the production cycle. Thirty-six calves had clinical signs and abnormal lung ultrasound scans (TRT, n = 36) and were promptly treated with florfenicol and meloxicam. Healthy veal calves without clinical signs and lung lesions were enrolled in the control group (CTR, n = 48), while 12 calves were excluded by the study. The clinical (Wisconsin and California scores), ultrasound and lung lesion scores, total lung consolidation area, treatment rates (success, relapse, mortality), average daily gain (ADG), carcass quality, and gross lesions of lungs at slaughterhouse were monitored. Results showed 91.7% of treatments were performed 3–28 days after arrival. Lung lesions occurred five days before the peak of clinical scores. Following treatment, lungs healed within 5 days with high treatment success rates (97.1% success rate in 45 days and 94.9% overall success rate without relapse). Finally, TRT had similar gross lung lesion prevalence, ADG, and carcass quality to CTR. These results suggest that early detection of BRD followed by a prompt treatment can lead to several key benefits for the health and the growth performances of veal calves.
Feline Adenovirus Isolate Shows Silent Nucleotide Alterations, Alternative Receptor/Coreceptor Binding, High Resistance to Disinfectants and Antiviral Drugs, as Well as Immunomodulation
Authors: Tarcsai Katalin Réka , Bányai Krisztián , Bali Krisztina , Abbas Anna Anoir , Kövesdi Valéria , Ongrádi József
Published: December 2024

Adenovirus (AdV) infection has been rarely documented in cats and other felids. Partial sequences of the hexon and fiber genes of a Hungarian feline adenovirus isolate (FeAdV isolate) showed a close relationship to human AdV (HAdV) type C1. Further molecular and biological characterization is reported here. Whole-genome sequencing revealed two silent mutations in the genome of the FeAdV isolate compared to a HAdV-C1 reference strain (at positions 14,096 and 15,082). Competitive antibody binding to the Coxsackie–adenovirus receptor and αvβ3 and αvβ5 integrin coreceptors inhibited the binding of the FeAdV isolate in different cell lines, but residual infections suggested alternative entry routes. The FeAdV isolate was found to be more sensitive to heat, low pH and detergents, but more resistant to alkaline and free chlorine treatments, as well as to ribavirin, stavudine and cidofovir treatments, than other human AdV types. We observed a suppression of IL-10 and TGF-β1 production during the entire course of viral replication. This immunomodulation may restore intratumoral immunity; thus, the FeAdV isolate could serve as an alternative oncolytic vector. Collectively, our results support that the Hungarian FeAdV isolate is a variant of common HAdV-C1. The cohabitation of cats with humans might result in reverse zoonotic infection. Felids appear to be susceptible to persistent and productive adenovirus infection, but further studies are needed to better understand the clinical and epidemiological implications.
Equine Asthma Diagnostics: Review of Influencing Factors and Difficulties in Diagnosing Subclinical Disease
Authors: Lendl Lioba , Barton Ann Kristin
Published: December 2024

This literature review focuses on diagnostics of equine asthma (EA), possible influencing factors on diagnostic techniques and latest developments in diagnosing horses during EA remission or with subclinical disease. Routine EA diagnostics include a clinical examination of the respiratory system with percussion and auscultation including a rebreathing examination, and clinical pathology including white blood cells and arterial blood gas analysis. Subsequent diagnostics include bronchoscopy to evaluate the amount and viscosity of respiratory secretion, bronchoalveolar lavage, and the cytology of tracheal aspirates (TAs) and bronchoalveolar lavage fluid (BALF). The grading of EA severity is built on respiratory effort at rest, which is increased in severe equine asthma. The inflammatory subtype is based on BALF cytology, while TA cytology helps to rule out previous bacterial infections. Different factors have an impact on the airways regarding the structure of the epithelium, cytology, and inflammatory markers possibly influencing the diagnosis of EA. Short-term exercise increases the total cell count and inflammatory mediators identified in the BALF of human patients, asymptomatic horses, and other species. Other factors involve cold or chlorinated air, long-term training effects, and concurrent additional respiratory disease, in particular exercise-induced pulmonary hemorrhage. As BALF cytology may be unremarkable during EA remission and low-grade disease, exercise tests and other factors stressing the bronchial epithelium may help to diagnose these patients.
Bread By-Product and Maize Silage as Alternative Ingredient Feeds for Production of Tenebrio molitor Larvae in High-Concentrate Substrates
Authors: Fondevila Guillermo , Remiro Ana , Remón Sara , Fondevila Manuel
Published: December 2024

Bread by-product and maize silage as substrates for Tenebrio molitor larvae were studied in four isonitrogenous diets: a control substrate (CTL) made of wheat grain, wheat bran, and soybean meal and three diets where wheat grain and wheat bran were partly substituted with bread by-product (BBP) or with this and either a low (MSL) or high (MSH) proportion of maize silage (170 or 310 g/kg, respectively). Larval weight was weekly monitored, and the chemical composition of larvae and residual substrates was analyzed at the end of the experiment. Larvae fed CTL and BBP grew more than those fed MSL and MSH (p < 0.001). Feed intake was greatest for BBP (p < 0.001), and consequently, feed-to-gain ratio (F:G) was better for larvae fed CTL than BBP, and better for BBP than MSL and MSH (p < 0.001). Besides, total production of crude protein and ether extract were greater for CTL and BBP than for MSH and MSL (p < 0.001). The inclusion of bread by-product in the substrate did not affect growth performance, whereas diets including maize silage impaired larval growth, substrate intake, and F:G. Both by-products can be advantageously used for feeding T. molitor larvae, but the impairments in growth performance should be considered to optimize production costs.
Polymorphisms of TXK and PLCE1 Genes and Their Correlation Analysis with Growth Traits in Ashidan Yaks
Authors: Zhang Juanxiang , Zha Xita , Yang Guowu , Ma Xiaoming , La Yongfu , Wu Xiaoyun , Guo Xian , Chu Min , Bao Pengjia , Yan Ping , Liang Chunnian
Published: December 2024

The tyrosine protein kinase (TXK) gene, as a member of the non-receptor tyrosine kinase Tec family, plays a vital role in signal transduction mediation. Phospholipase C epsilon 1 (PLCE1), a membrane-associated enzyme, is of paramount importance for the differentiation of myoblasts and the normal functioning of muscle tissue. In recent years, both of these genes have been reported to be associated with the economic traits of animals. This study aimed to investigate the relationship between single nucleotide polymorphisms (SNPs) in the TXK and PLCE1 genes and growth traits in Ashidan yaks and to search for potential molecular marker loci that can influence Ashidan yak breeding. A cGPS liquid microarray was utilized to genotype 232 Ashidan yaks and to analyze correlations between two SNP loci in the TXK and PLCE1 genes and yak body weight, body height, body length, and chest circumference at different periods. The results indicated that the g.55,999,531C>T locus of the TXK gene and the g.342,350T>G locus of the PLCE1 gene were significantly correlated with the growth traits of Ashidan yaks. Among these, individuals with the CC genotype at the g.55,999,531C>T locus showed a significantly higher body length at 6 months old compared to TT individuals, and those with the CT genotype at 12 months old had a significantly higher chest circumference than TT individuals. At the g.342,350T>G locus, the body height of GG genotype individuals at 18 months of age was significantly higher than that of TT genotype individuals and TG genotype individuals. The above findings can be used as theoretical support for the subsequent improvement of Ashidan yak breeding.
Quantification of Antimicrobial Use on Commercial Broiler Farms in Pakistan
Authors: Mahmood Qamer , Chantziaras Ilias , Dewulf Jeroen
Published: December 2024

Pakistan has a large, intensive broiler production industry, where antimicrobials are extensively used for both therapeutic and prophylactic purposes. Monitoring antimicrobial use (AMU) at the farm level is crucial to guide interventions for antimicrobial stewardship. The objective of this study was to comprehensively quantify AMU on commercial broiler farms in Pakistan using different metrics. Data on on-farm AMU (both therapeutic and prophylactic) were collected from intensive commercial broiler farms in Punjab, Pakistan. AMU was quantified using four different metrics: treatment incidence (TI) based on defined daily dose (TIDDDvetPK), TI based on defined course dose (TIDCDvetPK), TI based on used daily dose (TIUDDvetPK), and mg/kg biomass. The median TIDDDvetPK, TIDCDvetPK, TIUDDvetPK, and mg/kg biomass values were 57.7 (7.5–257.9), 13.3 (1.8–52.5), 75.3 (21.1–182.9), and 301 (46.8–1009.6), respectively, based on 741 group treatments given at 100 participating farms with 225 flocks. About 34% treatments were administered for therapeutic purposes, with 66% administered as prophylaxis. A total of 41 active substances, distributed across 17 antimicrobial classes, were identified from 139 commercial antimicrobial products used. The most commonly used active substances (AS), out of total 741 group treatments, were colistin (polymyxins) at 17%, enrofloxacin (quinolones) at 8%, neomycin (aminoglycosides) and amoxicillin (aminopenicillins) at 7% each, and procaine penicillin (NS penicillins) and streptomycin (aminoglycosides) at 6% each. According to the WHO categorization, 57% of the used AS were found to be critically important antimicrobials (CIAs) for human medicine. As many as 30% of treatments were administered in the first week (13% on the first day of production), and the main indication was prophylaxis for early chick mortality, followed by 21% in week 5, with main indication being feed supplements for necrotic enteritis. The average weight at slaughtering was 2.25 kg, gained after an average of 44 days of the production cycle. The alarmingly high AMU in Pakistani broiler production emphasizes the urgent need to reduce AMU, particularly involving CIAs and the prophylactic use of antimicrobials. This can be achieved by improving farm management practices and strengthening regulatory oversight to enable prudent antimicrobial use, eventually reducing the risk of AMR.
50 Years of Antibody Numbering Schemes: A Statistical and Structural Evaluation Reveals Key Differences and Limitations
Authors: Zhu Zirui , Olson Katherine S. , Magliery Thomas J.
Published: December 2024

Background: The complementarity-determining region (CDR) of antibodies represents the most diverse region both in terms of sequence and structural characteristics, playing the most critical role in antibody recognition and binding for immune responses. Over the past decades, several numbering schemes have been introduced to define CDRs based on sequence. However, the existence of diverse numbering schemes has led to potential confusion, and a comprehensive evaluation of these schemes is lacking. Methods: We employ statistical analyses to quantify the diversity of CDRs compared to the framework regions. Results: Comparative analyses across different numbering schemes demonstrate notable variations in CDR definitions. The Kabat and AbM numbering schemes tend to incorporate more conserved residues into their CDR definitions, whereas CDRs defined by the Chothia and IMGT numbering schemes display greater diversity, sometimes missing certain loop residues. Notably, we identify a critical residue, L29, within the kappa light chain CDR1, which appears to act as a pivotal structural point within the loop. In contrast, most numbering schemes designate the topological equivalent point in the lambda light chain as L30, suggesting the need for further refinement in the current numbering schemes. Conclusions: These findings shed light on regional sequence and structural conservation within antibody sequence databases while also highlighting discrepancies stemming from different numbering schemes. These insights yield valuable guidelines for the precise delineation of antibody CDRs and the strategic design of antibody repertoires, with practical implications in developing innovative antibody-based therapeutics and diagnostics.
Ellagic Acid Potentiates the Inhibitory Effects of Fluconazole Against Candida albicans
Authors: Mendes Amanda Graziela Gonçalves , Campos Carmem Duarte Lima , Pereira-Filho José Lima , Pereira Aleania Polassa Almeida , Reis Gabriel Silva Abrantes , Araújo Árlon Wendel de Marinho Silva , Monteiro Pablo de Matos , Vidal Flávia Castello Branco , Monteiro Silvio Gomes , da Silva Figueiredo Isabella Fernandes , Fernandes Elizabeth Soares , Monteiro Cristina de Andrade , Monteiro-Neto Valério
Published: December 2024

Background/Objectives: Antifungal resistance to azoles, coupled with the increasing prevalence of Candida albicans infections, represents a significant public health challenge and has driven the search for new natural compounds that can act as alternatives or adjuvants to the current antifungals. Ellagic acid (EA) has demonstrated antifungal activity; however, its effects are not fully understood. In this study, we investigated the in vitro anti-Candida activity of EA and its ability to potentiate the effects of fluconazole (FLZ) on C. albicans. Methods: The Minimum Inhibitory Concentration (MIC) of EA was determined by broth microdilution and its interaction with FLZ was assessed using a checkerboard assay. Additionally, we examined the effects of EA on yeast-to-hypha transition, inhibition of biofilm formation, time–kill kinetics, hemolytic activity, and cytotoxicity in HeLa ATCC® CCL-2™ cells. Results: EA exhibited MIC values ranging from 250 to 2000 µg/mL and showed synergistic and additive interactions with FLZ, resulting in a marked reduction in the MIC values of FLZ (up to 32-fold) and EA (up to 16-fold). In the time–kill assay, the most effective combinations were 4× EA MIC, 2× EA MIC, and FIC EA + FLZ, which showed fungicidal activity. Furthermore, EA did not show hemolytic activity and demonstrated low and dose-dependent cytotoxicity in HeLa cells, with no cytotoxic effects observed in combination with FLZ. EA and the synergistic combination of EA and FLZ interfered with both the yeast-to-hypha transition process in C. albicans cells and biofilm formation. In addition to its antifungal efficacy, EA demonstrated a favorable safety profile at the concentrations used. Conclusions: This study presents promising results regarding the potential use of EA in combination with FLZ for the treatment of C. albicans infections.
Antimicrobial Susceptibility Profiles of Commensal Escherichia coli Isolates from Chickens in Hungarian Poultry Farms Between 2022 and 2023
Authors: Kerek Ádám , Szabó Ábel , Jerzsele Ákos
Published: December 2024

Background: Widespread use of antibiotics has led to a global increase in resistance. The Escherichia coli bacterium is a facultative pathogen that often develops antibiotic resistance and is easily transmitted, not only in animal health but also in public health. Within the poultry sector, domestic fowl is widespread and one of the most dynamically growing sectors, which is why regular, extensive monitoring is crucial. Among economically important livestock, poultry as a major source of animal protein for humans is a frequent carrier of Escherichia coli, also with sporadically detected clinical disease. Methods: Our research evaluates the susceptibility of commensal Escherichia coli strains, isolated from large domestic fowl flocks in Hungary, to antibiotics of animal and public health importance, by determining the minimum inhibitory concentration value. Results: A total of 410 isolates were tested, with the highest level of resistance being found for florfenicol (62.7%). Particularly alarming are the resistance rates to enrofloxacin (52.9%), colistin (30.7%), and ceftriaxone (23.9%). We also found a resistance of 56.1% to amoxicillin and 22.2% to amoxicillin-clavulanic acid, which suggests that the majority of strains are β-lactamase-producing. When compared with the national human resistance data, we found with similar values for amoxicillin and amoxicillin-clavulanic acid, but the resistance rates of aminoglycosides, fluoroquinolones, and potency sulfonamide were worse in animal health. Conclusions: In conclusion, our results suggest that periodic surveys should be carried out and that long-term trends can be established that allow the monitoring of resistance patterns over time. For multidrug-resistant strains, new generation sequencing can be used to investigate the genetic background of resistance.
“Pleiotropic” Effects of Antibiotics: New Modulators in Human Diseases
Authors: Airola Carlo , Severino Andrea , Spinelli Irene , Gasbarrini Antonio , Cammarota Giovanni , Ianiro Gianluca , Ponziani Francesca Romana
Published: December 2024

Antibiotics, widely used medications that have significantly increased life expectancy, possess a broad range of effects beyond their primary antibacterial activity. While some are recognized as adverse events, others have demonstrated unexpected benefits. These adjunctive effects, which have been defined as “pleiotropic” in the case of other pharmacological classes, include immunomodulatory properties and the modulation of the microbiota. Specifically, macrolides, tetracyclines, and fluoroquinolones have been shown to modulate the immune system in both acute and chronic conditions, including autoimmune disorders (e.g., rheumatoid arthritis, spondyloarthritis) and chronic inflammatory pulmonary diseases (e.g., asthma, chronic obstructive pulmonary disease). Azithromycin, in particular, is recommended for the long-term treatment of chronic inflammatory pulmonary diseases due to its well-established immunomodulatory effects. Furthermore, antibiotics influence the human microbiota. Rifaximin, for example, exerts a eubiotic effect that enhances the balance between the gut microbiota and the host immune cells and epithelial cells. These pleiotropic effects offer new therapeutic opportunities by interacting with human cells, signaling molecules, and bacteria involved in non-infectious diseases like spondyloarthritis and inflammatory bowel diseases. The aim of this review is to explore the pleiotropic potential of antibiotics, from molecular and cellular evidence to their clinical application, in order to optimize their use. Understanding these effects is essential to ensure careful use, particularly in consideration of the threat of antimicrobial resistance.
Copper- and Iron-Based Nanoflowers in Cancer Theranostics
Authors: Vlachou Agathi , Gkika Despina A. , Efthymiopoulos Pavlos , Kyzas George Z. , Tsoupras Alexandros
Published: December 2024

In recent years, nanoscience and nanotechnology have gained prominence within materials science, offering new opportunities for cancer diagnosis and treatment. Advances in nanotechnology have allowed for the manipulation and size control of nanomaterials, leading to the development of a wide range of materials. The use of nanomaterials as chemical biology tools in cancer theranostics has been widely investigated, owing to their enhanced stability, biocompatibility, and improved cell permeability. These properties enable precise targeting while addressing the limitations of conventional cancer treatments. Nanoflowers, a specific class of nanomaterials, have recently attracted significant interest due to their promising properties for several biomedical applications. However, despite the growing attention toward nanoflowers, detailed reviews on the subject have been limited. This work focuses on two primary types of hybrid nanoflowers: iron- and copper-based ones. Within this article an overview of recent applications in cancer theranostics are thoroughly reviewed, while the synthesis processes for controlling morphology and size, underlying functions, and their characteristics and uses are also extensively explored, aiming to provide a guide for future developments in the field.
Exploring the Relationship Between Foot Position and Reduced Risk of Knee-Related Injuries in Side-Cutting Movements
Authors: Qu Xiuye , Xu Datao , Yang Xiaowei , Ugbolue Ukadike Chris , Zhou Huiyu , Gu Yaodong
Published: December 2024

Background: Knee-related injuries often result from poor movement patterns that destabilize the joint and increase stress on knee structures. Understanding the influence of foot positioning on knee biomechanics is critical for identifying high-risk movement patterns and preventing injuries. Methods: Twenty healthy male participants performed side-cutting movements at three different foot progression angles. One participant’s data were used to develop and validate a knee finite element model with high-speed dual fluoroscopic imaging (DFIS). Combined with a musculoskeletal analysis, the model simulated internal knee loads under various foot-positioning conditions. Results: The analysis revealed that, as the external foot progression angle increased, the ankle plantarflexion decreased, while the ankle internal rotation and knee valgus moments increased. Higher stress concentrations were observed on the ACL, lateral meniscus, lateral tibial cartilage, and medial collateral ligament, particularly at the femoral–tibial ACL attachments. Conclusion: The findings suggest that a toe-out foot position elevates the risk of knee injuries by increasing stress on key structures, whereas a toe-in position may enhance joint stability, reduce the ACL injury risk, and promote favorable muscle activation patterns.
Computational and Mathematical Methods for Neuroscience
Authors: Pisarchik Alexander N.
Published: December 2024

As our understanding of the brain continues to advance, so too does the demand for sophisticated tools that can model, simulate, and interpret the intricate data generated by contemporary neuroimaging and electrophysiological techniques [...]
System Design and Launch of a Hybrid Rocket with a Star-Fractal Swirl Fuel Grain Toward an Altitude of 15 km
Authors: Takano Atsushi , Yoshino Keita , Fukushima Yuki , Kitamura Ryuta , Funami Yuki , Takahashi Kenichi , Takahashi Akiyo , Kunihiro Yoshihiko , Miyake Makoto , Masai Takuma , Uemura Shizuo
Published: December 2024

To achieve low-cost and on-demand launches of microsatellites, the authors have been researching and developing a micro hybrid rocket since 2014. In 2018, a ballistic launch experiment was performed using the developed hybrid rocket, where it reached an altitude of about 6.2 km. The rocket engine had a 3D-printed solid fuel grain made of acrylonitrile butadiene styrene (ABS) resin in combination with a nitrous oxide oxidizer. The fuel grain port had a star-fractal swirl geometry in order to increase the surface area of the port, to promote the laminar–turbulent transition by increasing the friction resistance, and to give a swirling velocity component to the oxidizer flow. This overcame the hybrid rocket’s drawback of a low fuel regression rate; i.e., it achieved a higher fuel gas generation rate compared with a classical port geometry. In 2021, the hybrid rocket engine was scaled up, and its total impulse was increased to over 50 kNs; it reached an altitude of 15 km. In addition to the engine, other components were also improved, such as through the incorporation of lightweight structures, low-shock separation devices, a high-reliability telemetry device, and a data logger, while keeping costs low. The rocket was launched and reached an altitude of about 10.1 km, which broke the previous Japanese altitude record of 8.3 km for hybrid rockets. This presentation will report on the developed components from the viewpoint of system design and the results of the ballistic launch experiments.
Localized In Vivo Electro Gene Therapy (LiveGT)-Mediated Skeletal Muscle Protein Factory Reprogramming
Authors: Hensley Jacob , Francis Michael , Otten Alex , Korostyleva Nadezhda , Gagliardo Tina , Bulysheva Anna
Published: December 2024

Gene electrotransfer (GET) has gained significant momentum as a non-viral gene delivery method for various clinical applications, primarily in the cancer immunotherapy and vaccine development space. Preclinical studies have demonstrated exogenous gene delivery and expression in various tissues, including the liver, skin, cardiac muscle, and skeletal muscle. However, protein replacement applications of this technology have yet to be fully actuated. Plasmid DNA skeletal muscle delivery has been shown to maintain expression for up to 18 months. In the current study, we evaluated localized skeletal muscle delivery for protein replacement applications. We developed localized in vivo electro gene therapy (liveGT) protocols utilizing mono- and biphasic pulse sequences for localized pulse delivery directly to skeletal muscle with a custom monopolar platinum electrode. Plasmid DNA encoding human insulin and human glucokinase were chosen for this study to evaluate the liveGT platform for protein replacement potential. Initial in vitro GET was performed in mouse myoblasts to evaluate human insulin and glucokinase co-delivery. This was followed by liveGT-mediated reporter gene delivery in the skeletal muscle of Sprague–Dawley rats for pulse sequence selection. Protein replacement potential was evaluated in healthy (non-diabetic) rats with liveGT-mediated human insulin and glucokinase co-delivery to skeletal muscle. Human and rat insulin levels were measured via ELISA over the course of 3 months. Fed-state blood glucose measurements were monitored in correlation with serum human insulin levels. LiveGT-mediated skeletal muscle reprogramming successfully produced physiological levels of human insulin in serum over the course of 3 months. Hypo- and hyperglycemic events were not observed. Therefore, liveGT is a safe and viable platform for potential protein replacement therapies.
Experimental Determination of the Heat Transfer Coefficient of Wire Samples in an Industrial Lead Bath
Authors: Hof Jan , Reinicke Alexander , Büschgens Dominik , Wuppermann Christian
Published: December 2024

Steel wire is an important product in modern industries and is widely used in different applications. Where higher strength or durability is required, a heat treatment known as patenting is applied. This heat treatment uses molten lead as a coolant to form a fine-lamellar pearlite microstructure. Since lead is toxic, there are constant efforts to make the process environment as safe as possible as well as to replace lead baths. The first step for a possible replacement is a good understanding of the current boundary conditions. Therefore, an experimental setup was designed to measure the cooling curve of wire samples with different wire diameters and steel grades directly at two industrial lead baths. Furthermore, an inverse calculation method was developed to calculate the heat transfer coefficients from the cooling curves. The experimental setup, the evaluation method, and the resulting heat transfer coefficients are presented. The results show that the heat transfer coefficients vary with the sample diameter and velocity but not the lead bath temperature or steel grade. In addition, a comparison with correlations from literature was conducted.
Change in Spatiotemporal Parameters During Running at Different Degrees of Inclination: Systematic Review
Authors: Marszałek Patryk , Przednowek Krzysztof , França Cíntia , Martinho Diogo V. , Marques Adilson , Ferrari Gerson , Paśko Wojciech , Gouveia Élvio Rúbio
Published: December 2024

Background:Running is one of the simplest and most popular forms of exercise. Biomechanical evaluation of running is one of the elements of evaluating running technique and, consequently, improving sports performance. Running uphill and downhill is one of the components of daily running but also an element of training used by recreational runners. The aim of this study is to optimize running training and minimize the risk of injury by identifying changes in the spatiotemporal structure of running at different inclinations. Methods: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were followed. The protocol has been registered on the international platform INPLASY under the number INPLASY202430094U2. The search was conducted up to 30 March 2024 using the Scopus, PubMed, and Web of Science databases. Results: Spatiotemporal parameters were most frequently analyzed at 2.8–3.35 m/s velocities and inclinations in the range of −11% to 11%. Decreases in stride length (SL) and flight time (FT), and increases in step frequency (SF) were the most frequently reported changes from all parameters analyzed as a function of inclination and velocity. Significant increases or decreases in individual parameters were more often observed for positive inclination values than negative ones. Conclusions: The heterogeneous results of the study limit the possibility of determining the changes that occur in the spatiotemporal structure of the run under the impact of different inclinations. The variation in the results for negative inclination values indicates the different characteristics of running uphill and downhill. However, for uphill running, SF, SL, and FT are closely related to the increase in inclination.
Transition Effects in Bridge Structures and Their Possible Reduction Using Recycled Materials
Authors: Spyrowski Mariusz , Ostrowski Krzysztof Adam , Furtak Kazimierz
Published: December 2024

This article serves as a review of the current challenges in bridge engineering, specifically addressing the transition effect and the utilization of recycled materials. It aims to identify research gaps and propose innovative approaches, paving the way for future experimental studies. As a review article, the authors critically analyze the existing literature on the transition effects in bridge construction, their causes, and their negative impacts. Integral bridges are discussed as a solution designed to work in conjunction with road or rail embankments to transfer loads, minimizing maintenance and construction costs while increasing durability. Particular attention is given to the potential use of modified plastic composites as an alternative material in integral bridge structures. This concept not only addresses the issue of plastic waste but also promotes the long-term use of recycled materials, a key consideration given recycling limitations. This article highlights the importance of the connection between the embankment and the abutment and provides examples of polymer applications in bridge engineering. By outlining the state of the art, this review identifies future development paths in this niche, but promising, field. Almost 240 literature items were analyzed in detail, and works containing 475 different key words contained in about 3500 individual works were used for scientometric analysis. The results of the analysis clearly indicate the novelty of the presented subject matter.
A Seamless Technology Integration Framework for Elderly-Centered Interactive Systems: Design, Implementation, and Validation Through the Pillow Fight System
Authors: Lim Chor-Kheng , Chen Hung-Yu , Chen Xuan-Yu
Published: December 2024

The rapid aging of the global population has highlighted the urgent need for age-friendly technological solutions. However, our review of the existing studies revealed significant gaps between elderly users’ needs and the current smart technology products. While previous research has explored various aspects of elderly technology design, our systematic analysis indicated limitations, in terms of integrated frameworks, empirically validated interaction models, and long-term effectiveness evaluation. This study proposes a seamless technology framework for the development of elderly-centered interactive systems—named Pillow Fight—which we validated through a mixed-methods study of an innovative intergenerational gaming system. This research was conducted in two phases across 34 sites, with 1997 participants. Phase one (n = 659) established the framework’s effectiveness through systematic field testing, while phase two (n = 1338) validated its scalability and long-term benefits. The results highlight significant improvements in system usability scores (SUS), from individual use (77.85) to group activities (85.32), with intergenerational interaction achieving the highest scores (92.12). The integration of health monitoring features further enhanced learnability (94.12) usability (82.15). This study contributes to the design of technology for the elderly through (1) establishing an integrated theoretical framework for seamless technology integration, (2) developing and validating innovative intergenerational interaction models, and (3) providing empirical evidence through systematic field studies. These contributions could advance both theoretical understanding and practical applications while maintaining high user-friendliness.
Biochemical Characterization of Some Plum Cultivars Available on the Romanian Market
Authors: Micu Simona Mariana , Popoviciu Dan Răzvan , Grosu Maria Isabela , Radu Marius Daniel
Published: December 2024

Four plum cultivars commonly grown in Romania, ‘Angeleno’, ‘Anna Späth’, ‘Silvia’ and ‘Stanley’, were analyzed with regard to several parameters, including water content, juice pH, phenolic compounds (total and some major classes), carotenoids, ascorbic acid and soluble sugars. Among the four studied cultivars, ‘Silvia’ ranked the highest in terms of total phenolic content (82,345 mg/kg DW), flavonoids (43,171 mg/kg) and anthocyanins (1991 mg/kg). Hydroxybenzoic acids were found to be an important phenolic component; up to 33,971 mg/kg was found in ‘Angeleno’. The hydroxycinnamic acid content was highest in ‘Stanley’, and stilbenes were most prevalent in ‘Anna Späth’. Tannins were found in significant amounts only in fruit peel and were only present in some of the cultivars. Carotenoids were mostly present in the epicarp, with the maximum amount identified in ‘Anna Späth’ (313 mg/kg). The main compounds were β-carotene (of which the highest amount was identified in ‘Silvia’), lutein (with the highest amount found in ‘Silvia’) and lycopene (with the highest amount detected in ‘Stanley’). ‘Silvia’ also contained the highest amounts of ascorbic acid (7895 mg/kg in pulp and 12,229 mg/kg in peel). The highest soluble sugar content was found in the pulp of ‘Stanley’ (142,120 mg/kg), while ‘Anna Späth’ contained the lowest amount of soluble sugar. ‘Stanley’ also had less acidic pulp juice. Among the four cultivars, ‘Silvia’ ranked the highest in most classes of bioactive compounds, including total phenolic compounds, flavonoids, anthocyanins, tannins (peel only), β-carotene, lutein and ascorbic acid.
Fall Risk Assessment in Active Elderly Through the Use of Inertial Measurement Units: Determining the Right Postural Balance Variables and Sensor Locations
Authors: Nkizi Youssef , Thamsuwan Ornwipa
Published: December 2024

Falls among the elderly have been a significant public health challenge, with severe consequences for individuals and healthcare systems. Traditional balance assessment methods often lack ecological validity, necessitating more comprehensive and adaptable evaluation techniques. This research explores the use of inertial measurement units to assess postural balance in relation to the Berg Balance Scale outcomes. We recruited 14 participants from diverse age groups and health backgrounds, who performed 14 simulated tasks while wearing inertial measurement units on the head, torso, and lower back. Our study introduced a novel metric, i.e., the volume that envelops the 3-dimensional accelerations, calculated as the convex hull space, and used this metric along with others defined in previous studies. Through logistic regression, we demonstrated significant associations between various movement characteristics and the instances of balance loss. In particular, greater movement volume at the lower back (p = 0.021) was associated with better balance, while root-mean-square lower back angular velocity (p = 0.004) correlated with poorer balance. This study revealed that sensor location and task type (static vs. dynamic) significantly influenced the coefficients of the logistic regression model, highlighting the complex nature of balance assessment. These findings underscore the potential of IMUs in providing detailed objective balance assessments in the elderly by identifying specific movement patterns associated with balance impairment across various contexts. This knowledge can guide the development of targeted interventions and strategies for fall prevention, potentially improving the quality of life for older adults.