bluuebunny/crossref_metadata_2025 huggingface dataset:
Research progress of immunotherapy against anaplastic thyroid cancer
Authors: Chen Jiaqian , Xiao Zuixuan , Wu Hongyan
Published: March 2024

Anaplastic thyroid cancer (ATC) is the most aggressive type of thyroid cancer. While ATC is rare, its mortality is high. Standard treatments, such as surgery, radiotherapy, and chemotherapy, have demonstrated limited efficacy in managing ATC. However, the advent of immunotherapy has significantly improved the prognosis for patients with ATC. Immunotherapy effectively targets and eliminates tumor cells by using the power of the body’s immune cells. The neoantigen is an atypical protein generated by somatic mutation, is exclusively observed in neoplastic cells, and is devoid of central tolerance. Neoantigens exhibit enhanced specificity towards tumor cells and display robust immunogenic properties. Currently, neoantigen therapy is primarily applied in immune checkpoint inhibitors and cellular immunotherapy, encompassing adoptive immunotherapy and tumor vaccines. This study discusses the mechanism, tumor microenvironment, clinical trials, adverse events, limitations and future directions associated with ATC immunotherapy.
Elevated tumor necrosis factor alpha and vascular endothelial growth factor in intermediate age-related macular degeneration and geographic atrophy
Authors: Rajeswaren Vivian , Wagner Brandie D. , Patnaik Jennifer L. , Mandava Naresh , Mathias Marc T. , Manoharan Niranjan , de Carlo Forest Talisa E. , Gnanaraj Ramya , Lynch Anne M. , Palestine Alan G. ,   the University of Colorado Retina Research Group
Published: March 2024

Introduction

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine implicated in pathological changes to the retinal pigment epithelium that are similar to changes in geographic atrophy (GA), an advanced form of age related macular degeneration (AMD). TNF-α also modulates expression of other cytokines including vascular endothelial growth factor (VEGF), leading to choroidal atrophy in models of AMD. The purpose of this study was to investigate systemic TNF-α and VEGF in patients with GA and intermediate AMD (iAMD) compared to controls without AMD.

Methods

We examined plasma levels of TNF-α and VEGF in patients with GA, iAMD, and controls without AMD from the University of Colorado AMD registry (2014 to 2021). Cases and controls were characterized by multimodal imaging. TNF-α and VEGF were measured via multiplex immunoassay and data were analyzed using a non-parametric rank based linear regression model fit to plasma biomarkers.

Results

There were 97 GA, 199 iAMD patients and 139 controls. TNF-α was significantly increased in GA (Median:9.9pg/ml, IQR:7.3-11.8) compared to iAMD (Median:7.4, IQR:5.3-9.1) and in both GA and iAMD compared to controls (Median:6.4, IQR:5.3-7.8), p<0.01 for all comparisons. VEGF was significantly increased in iAMD (Median:8.9, IQR:4.8-14.3) compared to controls (Median:7.7, IQR:4.6-11.1), p<0.01. There was a significant positive correlation between TNF-α and VEGF in GA (0.46, p<0.01), and iAMD (0.20, p=0.01) with no significant interaction between TNF-α and VEGF in any group.

Discussion

These findings suggest TNF-α and VEGF may contribute to systemic inflammatory processes associated with iAMD and GA. TNF-α and VEGF may function as systemic biomarkers for disease development.
Reliability and modality analysis of patellar height measurement in pediatric knee
Authors: Kwak Yoon Hae , Park Soo-Sung , Huser Aaron J. , Kim Keunho , Koh Yong-Gon , Nam Ji-Hoon , Kang Kyoung-Tak
Published: March 2024

Introduction

Various measurement methods and imaging technique are in use to measure patellar height in pediatric patients. However, there is no gold standard as to which measurement method and modality are the most reliable for pediatric patients. Therefore, the aim of this study was to determine the inter-observer reliability, intra-observer reliability, and applicability of various patellar height measurement methods in pediatric knee. Additionaly, we analyzed the reliability across different imaging modalities.

Methods

Total 450 pediatric patients (age: 5–18 years) were evaluated using lateral knee radiographs and magnetic resonance imaging (MRI). The patellar height ratios were measured using five methods. Five methods were Insall–Salvati (IS), Koshino–Sugimoto (KS), Blackburne–Peel (BP), modified Insall–Salvati (MIS), and Caton–Deschamps (CD). The patients were categorized into two age groups: P (ages 5–13) and Q (ages 14–18). Each measurement was conducted twice by two raters. The intra-observer reliability, inter-observer reliability and inter-modality reliability were calculated. In addition, applicability was defined as the possibility to apply each measurement method to each age group.

Results

The KS method showed the highest inter-observer reliability and intra-observer reliability when using MRI for both age groups. The inter-observer reliability and intra-observer reliability of the IS for lateral knee radiographs was highest among all observers for group Q. The CD method showed the highest inter-observer reliability in group P, while the KS showed the highest intra-observer reliability in group P using lateral radiographs. The KS method showed the highest inter-modality reliability in group P, while the IS showed the highest inter-modality reliability in group Q. The KS method was applicable to all patients when using lateral knee radiography, and the IS method was applicable to all patients when using MRI.

Conclusions

Our results show that the reliability of various measurement method and imaging technique differed based on pediatric knee age group when measuring patellar height. Therefore, in the case of pediatric patients, reliability measurement methods and imaging techniques according to the patient's age should be applied.
The effect of oral butyrate on colonic short-chain fatty acid transporters and receptors depends on microbial status
Authors: Vagnerová Karla , Hudcovic Tomáš , Vodička Martin , Ergang Peter , Klusoňová Petra , Petr Hermanová Petra , Šrůtková Dagmar , Pácha Jiří
Published: March 2024

Butyrate, a metabolite produced by gut bacteria, has demonstrated beneficial effects in the colon and has been used to treat inflammatory bowel diseases. However, the mechanism by which butyrate operates remains incompletely understood. Given that oral butyrate can exert either a direct impact on the gut mucosa or an indirect influence through its interaction with the gut microbiome, this study aimed to investigate three key aspects: (1) whether oral intake of butyrate modulates the expression of genes encoding short-chain fatty acid (SCFA) transporters (Slc16a1, Slc16a3, Slc16a4, Slc5a8, Abcg2) and receptors (Hcar2, Ffar2, Ffar3, Olfr78, Olfr558) in the colon, (2) the potential involvement of gut microbiota in this modulation, and (3) the impact of oral butyrate on the expression of colonic SCFA transporters and receptors during colonic inflammation. Specific pathogen-free (SPF) and germ-free (GF) mice with or without DSS-induced inflammation were provided with either water or a 0.5% sodium butyrate solution. The findings revealed that butyrate decreased the expression of Slc16a1, Slc5a8, and Hcar2 in SPF but not in GF mice, while it increased the expression of Slc16a3 in GF and the efflux pump Abcg2 in both GF and SPF animals. Moreover, the presence of microbiota was associated with the upregulation of Hcar2, Ffar2, and Ffar3 expression and the downregulation of Slc16a3. Interestingly, the challenge with DSS did not alter the expression of SCFA transporters, regardless of the presence or absence of microbiota, and the effect of butyrate on the transporter expression in SPF mice remained unaffected by DSS. The expression of SCFA receptors was only partially affected by DSS. Our results indicate that (1) consuming a relatively low concentration of butyrate can influence the expression of colonic SCFA transporters and receptors, with their expression being modulated by the gut microbiota, (2) the effect of butyrate does not appear to result from direct substrate-induced regulation but rather reflects an indirect effect associated with the gut microbiome, and (3) acute colon inflammation does not lead to significant changes in the transcriptional regulation of most SCFA transporters and receptors, with the effect of butyrate in the inflamed colon remaining intact.
Optimum programmed intermittent epidural bolus interval time of ropivacaine 0.0625% with dexmedetomidine 0.4 μg/ml at a fixed volume of 10 mL: a randomized controlled trial
Authors: Mei Zhong , Wang Qingtao , Song Shaobo , Lu Wenying , Yu Jing
Published: March 2024

Background:

The aim of our study was to administer adequate local anesthetic in programmed intermittent epidural bolus (PIEB) to avoid breakthrough pain and decrease the use of manual and PCEA boluses. We, therefore, conducted this study to determine the effective PIEB interval time between boluses of ropivacaine 0.0625% with dexmedetomidine 0.4 μg/ml at a fixed volume of 10 mL in 90% of subjects (EI90), without the use of patient-controlled epidural analgesia (PCEA).

Methods:

A total of 80 subjects were included in the final statistical analysis from 23 August 2022 to 22 November 2022. The subjects were randomly assigned to one of four different PIEB time intervals: 40, 50, 60, and 70 min (groups 40, 50, 60, and 70), respectively. The primary outcome was the effective epidural labor analgesia, defined as no use of PCEA bolus or a manual bolus until the end of the first stage of labor or within 6 hours after loading dose administration. The PIEB EI90 (95% CI) between boluses of ropivacaine 0.0625% with dexmedetomidine 0.4 μg/ml at a fixed volume of 10 mL was estimated using probit regression.

Results:

The effective PIEB interval time between boluses of ropivacaine 0.0625% with dexmedetomidine 0.4 μg/ml at a fixed volume of 10 mL in 90% of subjects without the use of PCEA was 45.4 (35.5–50.5) minutes using probit regression. No statistical differences were found in the proportion of subjects with Bromage score > 0, hypotension, pruritus, nausea, and vomiting between groups. However, the highest sensory block (pinprick) in the 40-min group was significantly higher than that in the other groups.

Conclusion:

The estimated value for EI90 for PIEB between boluses of ropivacaine 0.0625% with dexmedetomidine 0.4 μg/ml at a fixed volume of 10 mL using probit regression was 45.4 (35.5–50.5) minutes. Furthermore, future studies are warranted to be established to determine the optimal parameters for different regimens in clinical practice.
Cross-modality feature fusion for night pedestrian detection
Authors: Feng Yong , Luo Enbo , Lu Hai , Zhai SuWei
Published: March 2024

Night pedestrian detection with visible image only suffers from the dilemma of high miss rate due to poor illumination conditions. Cross-modality fusion can ameliorate this dilemma by providing complementary information to each other through infrared and visible images. In this paper, we propose a cross-modal fusion framework based on YOLOv5, which is aimed at addressing the challenges of night pedestrian detection under low-light conditions. The framework employs a dual-stream architecture that processes visible images and infrared images separately. Through the Cross-Modal Feature Rectification Module (CMFRM), visible and infrared features are finely tuned on a granular level, leveraging their spatial correlations to focus on complementary information and substantially reduce uncertainty and noise from different modalities. Additionally, we have introduced a two-stage Feature Fusion Module (FFM), with the first stage introducing a cross-attention mechanism for cross-modal global reasoning, and the second stage using a mixed channel embedding to produce enhanced feature outputs. Moreover, our method involves multi-dimensional interaction, not only correcting feature maps in terms of channel and spatial dimensions but also applying cross-attention at the sequence processing level, which is critical for the effective generalization of cross-modal feature combinations. In summary, our research significantly enhances the accuracy and robustness of nighttime pedestrian detection, offering new perspectives and technical pathways for visual information processing in low-light environments.
Hyper-Kamiokande construction status and prospects
Authors: Abe Ko , Tanaka Hide-Kazu
Published: March 2024

The Hyper-Kamiokande (Hyper-K) project is the world’s leading international neutrino and nucleon decay experiment comprising a next-generation underground water Cherenkov detector and upgraded Japan Proton Accelerator Research Complex (J-PARC) neutrino beam. It will provide an enormous potential to discover the leptonic charge-parity violation, to investigate the Grand Unified Theory by proton decay exploration, and to determine the neutrino mass ordering. Further, Hyper-K will significantly enhance the capability to observe solar neutrinos and neutrinos from other astronomical sources in comparison with its predecessors. After the budget approval in January 2020, the Hyper-K project officially began, and the operation is expected to start in 2027. The excavations of a 2.0 km long access tunnel and the tunnels for the detector facility have been completed. In October 2022 it was started the excavation of one of the world’s largest underground caverns to suite the HK detector. This article discusses the status and the prospects of the Hyper-K detector construction.
Comparative transcriptome analysis reveals nicotine metabolism is a critical component for enhancing stress response intensity of innate immunity system in tobacco
Authors: Song Zhongbang , Wang Ruixue , Zhang Hongbo , Tong Zhijun , Yuan Cheng , Li Yong , Huang Changjun , Zhao Lu , Wang Yuehu , Di Yingtong , Sui Xueyi
Published: March 2024

The pyridine alkaloid nicotine acts as one of best-studied plant resistant traits in tobacco. Previous research has shown that NtERF199 and NtERF189, acting as master regulators within the NIC1 and NIC2 locus, quantitatively contribute to nicotine accumulation levels in N. tabacum. Genome editing-created Nic1(Nterf199) and Nic2 (Nterf189) double mutant provides an ideal platform for precisely dissecting the defensive role of nicotine and the connection between the nicotine biosynthetic pathway with other putative metabolic networks. Taking this advantage, we performed a comparative transcriptomic analysis to reevaluate the potential physiological and metabolic changes in response to nicotine synthesis defect by comparing the nic1nic2 and NIC1NIC2 plants. Our findings revealed that nicotine reduction could systematically diminishes the expression intensities of genes associated with stimulus perception, signal transduction and regulation, as well as secondary metabolic flux. Consequently, this global expression reduction might compromise tobacco adaptions to environmental fitness, herbivore resistances, and plant growth and development. The up-regulation of a novel set of stress-responsive and metabolic pathway genes might signify a newly established metabolic reprogramming to tradeoff the detrimental effect of nicotine loss. These results offer additional compelling evidence regarding nicotine’s critical defensive role in nature and highlights the tight link between nicotine biosynthesis and gene expression levels of quantitative resistance-related genes for better environmental adaptation.
The association between family adaptability and adolescent depression: the chain mediating role of social support and self-efficacy
Authors: Lin Yanyan , Jia Guangyunxian , Zhao Zirong , Li Meng , Cao Guanghai
Published: March 2024

Objective

Previous research has shown a correlation between family adaptability and adolescent depression. However, there is a lack of studies that have investigated the underlying mechanism between family adaptability and adolescent depression. Based on the Ecological Systems Theory, this study aims to investigate the link between family adaptability and depression in adolescents, mediated by the sequential roles of social support and self-efficacy.

Methods

The sample consisted of 1086 students randomly selected from seven public middle schools in Shandong Province, Eastern China. All the participants filled in the structured self-report questionnaires on family adaptability, social support, self-efficacy, and depression. The data were analyzed using SPSS 25.0 and Structural Equation Modeling (SEM) in AMOS 24.0.

Results

The findings of this study are as follows: (1) Family adaptability is negatively associated with adolescent depression; (2) Social support plays a mediating role between family adaptability and adolescent depression; (3) Self-efficacy plays a mediating role between family adaptability and adolescent depression; (4) Social support and self-efficacy play a chain mediation role between family adaptability and adolescent depression.

Conclusion

It is suggested that early interventions and support should be provided to facilitate adolescents’ family adaptability, social support, and self-efficacy, thus reducing their depression and improving mental health of adolescents.
The effect of school size and class size on school preparedness
Authors: Antoniou Faye , Alghamdi Mohammed H. , Kawai Kosuke
Published: March 2024

The purpose of the present study was to understand students’ school readiness as a function of student and teacher behaviors but also school size and class size using both linear and non-linear analytical approaches. Data came from 21,903 schools distributed across 80 countries as per the 2018 cohort of the PISA database. Results pointed to a preference for the Cusp model in that the relationship between school and class sizes with achievement proved to be best described by the non-linearity of the Cusp catastrophe model. The critical benchmarks were a school size of 801 students and a class size of 27 students for which increases beyond those thresholds were linked to nonlinearity and unpredictability in school readiness. For this reason, we suggest using the cusp catastrophe model from Nonlinear Dynamical Systems Theory (NDST) to understand more fully such complex phenomena.
A qualitative internet-based study of parental experiences of adolescents suffering from affective disorders with non-suicidal self-injury during the COVID-19 pandemic
Authors: Wang Yongna , Chen Xueqiu , Song Chun , Wu Yan , Liu Lihua , Yang Lili , Hao Xuege
Published: March 2024

Objective

Non-suicidal self-injury (NSSI) behaviors of adolescents with affective disorders can directly deteriorate parents’ internal experiences, and negative parental experiences can exacerbate or even worsen NSSI behaviors. This study investigates the impact of NSSI behaviors exhibited by adolescents with affective disorders on the internal experiences of parents. Specifically, our research focuses on the inner experiences of parents when their children engage in NSSI behaviors during social isolation of the COVID-19, offering insights for addressing parental mental health issues related to NSSI and developing positive parental behavioral models to optimize adolescent behavior during major public health events.

Methods

Semi-structured interviews were conducted with 21 parents of adolescents with affective disorders displaying NSSI behaviors during the COVID-19 pandemic. The Colaizzi 7-step analysis was employed to refine and categorize emerging themes.

Results

Our study revealed that parents of adolescents facing NSSI during the COVID-19 pandemic underwent different internal experiences, which could be classified into four themes: negative experience, high caregiving burden, lack of caregiving capacity, and resilience.

Conclusion

This Internet-based research is the first to explore the internal experiences of parents of adolescents with affective disorders experiencing NSSI during the COVID-19 pandemic. It sheds light on how parents, in response to their children’s NSSI behaviors, undergo resilience following negative experiences, explore more open and supportive family model. Despite these positive outcomes, parents express a need for increased knowledge about NSSI illness care and a desire for professional assistance.
The relationship between executive function and the association of motor coordination difficulties and social communication deficits in autistic children
Authors: Gu Tingfeng , Jin Chengkai , Lin Lizi , Wang Xin , Li Xiuhong , Jing Jin , Cao Muqing
Published: March 2024

Background

Motor coordination difficulties could contribute to social communication deficits in autistic children. However, the exploration of the mechanism implicated in these claims has been limited by the lack of potential confounders such as executive function (EF).

Methods

We investigated the role that EF plays in the relationship between motor coordination and social communication in a school-aged autistic population via a structural model in a statistically robust manner. The results of questionnaires, including the Developmental Coordination Disorder questionnaire, the Behavior Rating Inventory of Executive Function, and the Social Responsiveness Scale, were collected to measure motor coordination, social communication deficits, and EF.

Results

A total of 182 autistic children (7.61±1.31 years, 87.9% boys) were included in the final analysis. In the model with EF as a mediator, the total effect (β=-0.599, P<0.001) and the direct effect (β=-0.331, P =0.003) of motor coordination function on social communication were both significant among autistic children without intellectual disability (ID), as were indirect effects through EF (β=-0.268, P<0.001).

Conclusion

EF partially mediates the motor coordination and social communication correlation among autistic children. We suggest that motor coordination should be included in the routine evaluation of autistic surveillance and rehabilitation procedures.
Visual Schema Displacement Therapy versus Eye Movement Desensitization and Reprocessing therapy versus waitlist in the treatment of post-traumatic stress disorder: results of a randomized clinical trial
Authors: Matthijssen Suzy J. M. A. , Brouwers Thomas C. , de Jongh Ad
Published: March 2024

Introduction

Visual Schema Displacement Therapy (VSDT) is a novel approach showing promise in mitigating distressing memories, akin to Eye Movement Desensitization and Reprocessing (EMDR).

Objectives

This study aimed to determine the safety, feasibility, and effectiveness of VSDT in individuals with post-traumatic stress disorder (PTSD), comparing it to EMDR therapy and a waitlist control condition (WLCC). It was hypothesized that the application of VSDT would be safe and PTSD symptoms significantly be reduced from both baseline to post-treatment and from baseline to follow-up in the VSDT and EMDR therapy conditions. Furthermore, we expected both treatments to be significantly more effective than the waitlist control. Moreover, we hypothesized that VSDT and EMDR therapy would be associated with significant improvements in symptoms of depression and general psychopathology.

Method

Forty-six adults with PTSD were randomly assigned to VSDT, EMDR therapy, or WLCC, receiving six 90-minute sessions. Assessments included the Clinician Administered PTSD Scale for the Diagnostic Statistical Manual (DSM)-5 (CAPS-5), PTSD Checklist for DSM-5 (PCL-5), Beck Depression Inventory-II (BDI-II) and Brief Symptom Inventory (BSI) before, during, and 3 months post-treatment.

Results

Bayesian analysis found no differences between VSDT and EMDR in PTSD symptom reduction but both outperformed WLCC. EMDR was superior to the WLCC in reducing symptoms of depression and general psychopathology. At 3-month follow-up, 58.3% of the participants in the VSDT condition no longer met the PTSD diagnostic criteria (41.2% EMDR therapy and 15.4% WLCC) with no difference between the two therapy conditions. Self-reported PTSD symptom reduction was significant in VSDT (d = 1.38) and EMDR (d = 1.40) but modest in WLCC (d = 0.39). Dropout rate was 19.3%, with no adverse events.

Conclusion

This study supports VSDT’s efficacy in treating PTSD, offering a valuable therapeutic option comparable to EMDR, with significant reductions in PTSD symptoms and no difference with EMDR or the control condition for depressive symptoms and general psychopathology, and no reported adverse events.
Surveillance for adverse events following immunization with DTaP-containing combination vaccines in Linping, China, 2019–2022
Authors: Chen Qinghua , Zhang Chuandi , Ye Chunmei , Zhu Junwei , Shen Jie , Zhu Chang , Yang Pai , Liu Tiane , Xu Yuyang
Published: March 2024

Background

The DTaP-Hib and DTaP-IPV/Hib combination vaccine can be used as a substitute for the diphtheria, tetanus, and acellular pertussis combined vaccine (DTaP). We aimed to evaluate the safety of multi-component vaccines containing DTaP by analyzing the reporting rates and characteristics of adverse events following immunization (AEFIs) in Linping District during the years 2019 to 2022.

Methods

We obtained data of AEFI and vaccination from the National AEFI Surveillance System of China and Zhejiang Municipal Immunization Information Management System, respectively, during 2019–2022 for a descriptive, epidemiological analysis.

Results

The total number of AEFI reported following vaccinations with DTaP-containing combination vaccines was 802 in Linping District from 2019 to 2022. The overall reporting rates of AEFIs following DTaP, DTaP-Hib, and DTaP-IPV/Hib vaccinations were 445.72 (537 cases), 536.29 (45 cases), and 306.13 (220 cases) per 100,000 doses in Linping District from 2019 to 2022, respectively. Only one case of a serious AEFI following DTaP vaccination, with a reporting rate of 0.83 per 100,000 doses. The composition ratio of vaccine product-related reactions for DTaP, DTaP-Hib, and DTaP-IPV/Hib were 99.81, 97.78, and 100.00%, respectively. The composition ratio of coincidental events for DTaP and DTaP-Hib were 0.19 and 2.22%, respectively. The reporting rates of total AEFIs for DTaP-IPV/Hib were lower than for DTaP. The reporting rate of local induration for DTaP-Hib was lower than for DTaP, and the reporting rates of local redness & swelling and local induration for DTaP-IPV/Hib were both lower than for DTaP. DTaP-IPV/Hib had a higher proportion of AEFIs in first quarter compared to DTaP. The reporting rate after the second dose of DTaP-Hib was higher than that of DTaP, and the reporting rates of AEFIs after the first dose and third dose of DTaP-IPV/Hib were lower than DTaP.

Conclusion

The reported AEFIs to multi-component vaccines containing DTaP components during 2019–2022 in Linping District were mainly mild vaccine reactions. DTaP-containing combination vaccines demonstrated a good safety profile.
Evaluation of SINERGIAPS, an intervention to improve patient safety in primary healthcare centers in Spain based on patients’ perceptions and experiences: a protocol for a hybrid type I randomized clinical trial
Authors: Fiol-deRoque Maria A. , Mansilla Georgina Vidal , Maderuelo-Fernández José A. , Tamayo-Morales Olaya , Martín-Luján Francisco , Astier-Peña Pilar , Chacón-Docampo Macarena , Orrego Carola , Gens-Barberà Montserrat , Andreu-Rodrigo Pilar , Ricci-Cabello Ignacio ,   the SinergiAPS team
Published: March 2024

Background

Adverse events in the primary care setting result in a direct cost equivalent to at least 2.5% of total healthcare spending. Across OECD countries, they lead to more than seven million avoidable hospital admissions annually. In this manuscript, we describe the protocol of a trial aimed at evaluating the effectiveness of SinergiAPS (a patient-centered audit and feedback intervention) in reducing avoidable hospital admission and explore the factors that may affect its implementation.

Methods

We will conduct a 24-month, parallel, open-label, multicenter, pragmatic, hybrid type 1 randomized clinical trial. 118 primary healthcare centers with wide geographical distribution in Spain will be randomly assigned (ratio 1:1) to two groups. The intervention group will receive two audits (baseline and intermediate at 12 months) based on information collected through the administration of the PREOS-PC questionnaire (a measure of patient-reported patient safety) to a convenience sample of 100 patients per center. The intervention group will receive reports on the results of both audits, along with educational resources aimed at facilitating the design and implementation of safety improvement plans. The control group will receive care as usual. The primary outcome will be the rate of avoidable hospitalizations (administrative data). Secondary outcomes: patient-reported patient safety experiences and outcomes (PREOS-PC questionnaire); patient safety culture as perceived by professionals (MOSPSC questionnaire); adverse events reported by healthcare professionals (ad hoc questionnaire); the number of safety improvement actions which the re has implemented (ad hoc questionnaire). Outcome data will be collected at baseline and 24 months follow-up. For the evaluation of the implementation of the SinergiAPS intervention, we will draw on the Consolidated Framework for Implementation Research (CFIR). We will collect and analyze qualitative and quantitative data (30 individual interviews, implementation logbooks; questionnaires for professionals from intervention centers, and level of use of the SinergiAPS web tool).

Discussion

This study will expand the scarce body of evidence existing regarding the effects and implementation of interventions aimed at promoting patient and family engagement in primary healthcare, specifically for enhancing patient safety. The study has the potential to produce an impact on clinical practice, healthcare systems, and population health.

Clinical Trial Registration: https://clinicaltrials.gov/study/NCT05958108?term=sinergiAPS&rank=1 (NCT05958108).
Temporal variation of chronic child malnutrition in the context of strengthening healthcare services in Burkina Faso: an Oaxaca-Blinder multivariate decomposition analysis
Authors: Sawadogo Pengdewendé Maurice , Kobiané Jean-François , Tchouaket Nguemeleu Eric
Published: March 2024

Malnutrition seriously affects children’s health, survival, and future productivity. According to the literature, increasing the supply of health services should help reduce the spread of malnutrition. This article analyses the sources of changes in the decline of chronic malnutrition during the 2000s, where there was an increase in the supply of health services in Burkina Faso. We used data from demographic and health surveys conducted in 2003 and 2010 in Burkina Faso. Malnutrition was defined according to the recommendations of the World Health Organization, while using standards of growth which are current and uniform for the two periods of study considered. We analyzed the source of temporal variation of chronic malnutrition through the Oaxaca-Blinder multivariate decomposition of the proportion of children suffering from chronic malnutrition. The analyses showed that the relative extent of chronic malnutrition in children decreased significantly, from 43.4% (CI 95%: 42.3–44.4) in 2003 to 34.7% (CI 95%: 33.6–35.9) in 2010. A quarter of this variation is due to a change in characteristics (composition effect), and the remaining 74.74% is due to a difference in coefficients (performance or behavior effect). Improved access to health services played a crucial role in reducing the scale of chronic malnutrition between 2003 and 2010. Other factors, such as educating mothers and urbanization, also contributed significantly. This study shows that improving access to health services is crucial for reducing chronic malnutrition. So, programs tackling child malnutrition must first and foremost ensure that children have access to health services.
Decoding lifespan secrets: the role of the gonad in Caenorhabditis elegans aging
Authors: Pires da Silva Andre , Kelleher Rhianne , Reynoldson Luke
Published: March 2024

The gonad has become a central organ for understanding aging in C. elegans, as removing the proliferating stem cells in the germline results in significant lifespan extension. Similarly, when starvation in late larval stages leads to the quiescence of germline stem cells the adult nematode enters reproductive diapause, associated with an extended lifespan. This review summarizes recent advancements in identifying the mechanisms behind gonad-mediated lifespan extension, including comparisons with other nematodes and the role of lipid signaling and transcriptional changes. Given that the gonad also mediates lifespan regulation in other invertebrates and vertebrates, elucidating the underlying mechanisms may help to gain new insights into the mechanisms and evolution of aging.
Differential enrichment of bacteria and phages in the vaginal microbiomes in PCOS and obesity: shotgun sequencing analysis
Authors: Zheng Senlin , Chen Huimin , Yang Hongyi , Zheng Xulan , Fu Tengwei , Qiu Xiaoyan , Wang Meiqin
Published: March 2024

Introduction

Previous research has linked vaginal bacteria to polycystic ovary syndrome (PCOS) and obesity in women, yet the specific disparities in vaginal microbiota between these conditions remain unclear.

Methods

In this study, we aimed to elucidate the contribution of dysregulated vaginal microbiota to PCOS and obesity by analyzing the vaginal microbiota in reproductive-aged women with and without PCOS, as well as obese and non-obese women, using shotgun sequencing.

Results

Swab specimens were collected from four groups of subjects: PCOS and obese, PCOS and non-obese, non-PCOS and obese, and non-PCOS and non-obese. A total of 333 bacteria and 24 viruses/phages were identified to the species level. Clustering analysis revealed that non-PCOS and non-obese individuals exhibit a similar “healthy” vaginal microbiome, while both obesity and PCOS were associated with microbial dysbiosis. Significant differences in abundance were observed for 26 bacterial species and 6 phages/viruses between groups. Notably, pathobionts such as Streptococcus pyogenes, Leptospira santarosai, Citrobacter amalonaticus, Listeria ivanovii, and Clostridium perfringens were significantly less abundant or absent in the non-PCOS and non-obese group. Furthermore, the abundance of Lactobacillus, Pseudomonas bacteria, and their corresponding phages exhibited positive correlations. Lactobacillus bacteria, lactobacillus phage, and pseudomonas phage/virus were identified as indicators of a healthy vaginal microbiome. Importantly, the differentially enriched bacteria in the PCOS and obesity groups were distinct.

Discussion

This study confirms that PCOS and obesity are associated with differing enrichment of bacteria and viruses/phages, with both conditions linked to microbial dysbiosis. Moreover, our findings suggest that vaginal phage diversity is associated with a healthy vaginal microbiota, while dysbiosis is associated with a decrease in phages alongside increased bacterial diversity.
Exploring linkages: addressing the relationship between the climate crisis and HIV prevention with recommendations for emerging pre-exposure prophylaxis programs
Authors: Williams Katie M. , Olisa Adaobi Lisa , Wilcher Rose
Published: March 2024

Short- and long-term impacts of the climate crisis continue to be felt across the public health landscape. Many individuals marginalized by the climate crisis also navigate a higher likelihood of exposure to HIV. By understanding this relationship, we can better position HIV prevention, and pre-exposure prophylaxis (PrEP) programs specifically, to meet user needs in communities experiencing the effects of the climate crisis. In support, we propose four recommendations for mitigating the impact of the climate crisis on those who may benefit from PrEP: (1) leverage existing and emerging research and lived experience to intentionally target and appropriately reach individuals affected by the climate crisis who may need or want PrEP; (2) emphasize the need for more climate-resilient PrEP products within the research and development pipeline; (3) build a continued understanding of the role of the climate crisis-HIV relationship in product introduction through national collaboration; and (4) strengthen the integration of PrEP service delivery and response to intimate partner violence. The PrEP market is set for rapid expansion with the introduction of new prevention methods to enable choice. To be comprehensively responsive to potential PrEP users, we must consider and address how the climate crisis changes not only the environmental landscape, but the prevention ecosystem.
Electric vehicles adoption challenges in Oman: a comprehensive assessment and future prospects for sustainable cities
Authors: Ahmed Umar , Fida Bashir Ahmad , Thumiki Venkat Ram Raj , Hamdun Al Marhoobi Sauda Salim
Published: March 2024

The rising popularity of Electric Vehicles (EVs), fuelled by technological advancements and supportive government policies, presents a promising solution to reduce carbon emissions from conventional cars. This study explores public perceptions of obstacles to Electric Vehicle (EV) adoption in Oman and assesses EVs' impact on city sustainability. Over 300 car consumers in Muscat participated in a survey, facilitated through Microsoft Forms, and distributed via email and social media platforms like WhatsApp, Facebook, LinkedIn, and Instagram. Data analysis utilized descriptive statistics and Structural Equation Modelling (SEM). The study finds that the main obstacles to EV adoption in Oman include a lack of public charging infrastructure, limited EV knowledge, and concerns about available EV models. Safety issues, financial constraints, and low public awareness of climate change also hinder adoption. Structural SEM results show positive correlations between cost-effectiveness, charging infrastructure availability, climate change awareness, and intention to adopt EVs. However, safety concerns negatively impact EVs purchase intention. Regarding the impact of EVs on Oman's cities, respondents generally believe in the potential for EVs to improve air quality, enhance urban living conditions, and mitigate noise and air pollution. However, the respondents were also cautious about the widespread use of EVs due to the high infrastructure investment costs and energy consumption. Therefore, this study recommends targeted interventions like awareness campaigns, better charging infrastructure, and adoption incentives. These insights are vital for policymakers and stakeholders aiming to promote sustainable EV adoption in Oman and beyond.
Spatial and temporal analysis of African swine fever front-wave velocity in wild boar: implications for surveillance and control strategies
Authors: Martínez Avilés Marta , Montes Fernando , Sacristán Irene , de la Torre Ana , Iglesias Irene
Published: March 2024

The front-wave velocity of African swine fever (ASF) virus spread is depicted through a retrospective spatial and temporal analyses of wild boar outbreaks from Jan. 2014 to Jan. 2022 in Estonia, Latvia, Lithuania and Eastern Poland—regions responsible for more than 50% of all wild boar cases in the EU. The study uses empirical semivariograms in a universal kriging model to assess spatial autocorrelation in notification dates and identifies a discernable large-scale spatial trend. The critical parameter of ASF front-wave velocity was identified (Mean = 66.33 km/month, SD = 163.24) in the whole study area, and explored the variations across countries, wild boar habitat suitability, seasons, and the study period. Statistical differences in front-wave velocity values among countries and temporal clusters are explored, shedding light on potential factors influencing ASF transmission dynamics. The implications of these findings for surveillance and control strategies are discussed.
Research Progress on Thin-Walled Sound Insulation Metamaterial Structures
Authors: Zhang Yumei , Zhang Jie , Li Ye , Yao Dan , Zhao Yue , Ai Yi , Pan Weijun , Li Jiang
Published: March 2024

Acoustic metamaterials (AMs) composed of periodic artificial structures have extraordinary sound wave manipulation capabilities compared with traditional acoustic materials, and they have attracted widespread research attention. The sound insulation performance of thin-walled structures commonly used in engineering applications with restricted space, for example, vehicles’ body structures, and the latest studies on the sound insulation of thin-walled metamaterial structures, are comprehensively discussed in this paper. First, the definition and math law of sound insulation are introduced, alongside the primary methods of sound insulation testing of specimens. Secondly, the main sound insulation acoustic metamaterial structures are summarized and classified, including membrane-type, plate-type, and smart-material-type sound insulation metamaterials, boundaries, and temperature effects, as well as the sound insulation research on composite structures combined with metamaterial structures. Finally, the research status, challenges, and trends of sound insulation metamaterial structures are summarized. It was found that combining the advantages of metamaterial and various composite panel structures with optimization methods considering lightweight and proper wide frequency band single evaluator has the potential to improve the sound insulation performance of composite metamaterials in the full frequency range. Relative review results provide a comprehensive reference for the sound insulation metamaterial design and application.
Detection of the Lassa Virus in a Group of Odontogenic Bone Tumor Tissues
Authors: de Feo Marco , Dilu Tamba Frédéric , Makaka Mutondo Anguy , Kashitu Mujinga Gracia , Odong Opiyo Stephen , Castellani Chiara , Pavesi Luca , Mpingabo Patrick I. , Ahuka-Mundeke Steve , Di Agostino Silvia
Published: March 2024

Odontogenic bone tumor (OT) is a rare pathology in the world, but it is very common in developing countries; its etiology is still unknown, and it causes serious deformities of the mandible and maxilla if it is not operated upon soon. Lassa virus (LASV) belongs to the Arenaviridae family, and its reservoir is a rodent of the genus Mastomys. The transmission of the LASV to humans can occur through ingestion or inhalation by contact with dirty objects, the consumption of contaminated food, or exposure to wounds, as rodents shed the virus in their urine and excrement. In this observational study, we aim to evaluate the presence of LASV in OT patient tissues collected in the Democratic Republic of the Congo. For this purpose, a group of nine patients affected by OT were enrolled, and the tissues derived from the surgery were collected. In total, 81.5% of the tissues were positive for LASV presence. Interestingly, we found that not only was the tumor LASV-positive, but in some cases, the bone was close to the tumor and the oral mucosa lining. These preliminary data could suggest the hypothesis that LASV may be involved with the onset of OT.
Marginal Adaptation and Porosity of a Novel MTA Brand Applied as Root-End Filling Material: A Micro-CT Study
Authors: Kouzmanova Yaneta , Dimitrova Ivanka
Published: March 2024

Marginal adaptation and internal porosity characteristics of root-end filling materials are important factors determining their clinical performance. The aim of this study was to quantitatively evaluate the marginal adaptation to radicular dentin (interfacial void volume) and internal porosity volume of a novel mineral trioxide aggregate brand using micro-CT analysis. Ten extracted upper central incisors were selected, instrumented, and obturated. Roots were resected at the apical 3 mm, and root-end cavities were prepared ultrasonically and filled with Harvard MTA. SkyScan 1272 micro-CT equipment was used to scan the specimens at a resolution of 6 μm, and three-dimensional images were reconstructed. All volumetric porosity parameters of the tested material were calculated in absolute (mm3) and relative values (%), as follows: open porosity volume (OPV), closed porosity volume (CPV), and total porosity volume (TPV). The mean OPV and OPV% found for Harvard MTA were 0.0268 mm3 and 0.91%, respectively. The mean CPV and CPV% were 0.0283 mm3 and 0.94%, respectively. The TPV and TPV% were 0.0569 mm3 and 1.85%. There was no significant difference between the OPV% and CPV% (p < 0.05). In conclusion, when applied as a retrofilling material, Harvard MTA exhibited excellent marginal adaptation to the dentin with minimal interfacial voids and internal microporosity. Therefore, this new calcium silicate brand may be considered an efficient alternative to conventional products.
Correction: Berceanu, C.; Pătrașcu, M. Engineering Emergence: A Survey on Control in the World of Complex Networks. Automation 2022, 3, 176–196
Authors: Berceanu Cristian , Pătrașcu Monica
Published: March 2024

In the original publication [...]
Real-Time Monitoring of Thermal Phenomena during Femtosecond Ablation of Bone Tissue for Process Control
Authors: Al-Bourgol Samy , Machinet Guillaume , Bakkali Aboubakr , Faucon Marc , Gemini Laura
Published: March 2024

Femtosecond (fs) laser technology is currently being considered in innovative fields such as osteotomy and treatment of hard tissue thanks to the achievable high resolution and ability to prevent tissue damage. In a previous study, suitable process parameters were obtained to achieve competitive ablation rates on pork femur processing. Nevertheless, a better control of thermal accumulation in the tissue during laser ablation could further improve the postoperative regeneration of the treated bone compared with conventional procedures and push forward the exploitation of such technology. This study presents methods for real time analyses of bone tissue temperature and composition during fs laser ablation and highlights the importance of implementing an efficient cooling method of bone tissue in order to achieve optimized results. Results show that it is possible to achieve a larger process window for bone tissue ablation where bone tissue temperature remains within the protein denaturation temperature in water-based processing environment. This is a key outcome towards a clinical exploitation of the presented technology, where higher process throughputs are necessary. The effects of process parameters and environments on bone tissue were confirmed by LIBS technique, which proved to be an efficient method by which to record real-time variation of bone tissue composition during laser irradiation.
Development of a Probability-Based In Vitro Eye Irritation Screening Platform
Authors: Arora Seep , Goralczyk Anna , Andra Sujana , Lim Soon Yew John , Toh Yi-Chin
Published: March 2024

Traditional eye irritation assessments, which rely on animal models or ex vivo tissues, face limitations due to ethical concerns, costs, and low throughput. Although numerous in vitro tests have been developed, none have successfully reconciled the need for high experimental throughput with the accurate prediction of irritation potential, attributable to the complexity of irritation mechanisms. Simple cell models, while suitable for high-throughput screening, offer limited mechanistic insights, contrasting with more physiologically relevant but less scalable complex organotypic corneal tissue constructs. This study presents a novel strategy to enhance the predictive accuracy of screening-compatible simple cell models in eye irritation testing. Our method combines the results of two in vitro assays—cell apoptosis and nociceptor (TRPV1) activation—using micropatterned chips to partition human corneal epithelial cells into numerous discrete small populations. Following exposure to test compounds, we measure apoptosis and nociceptor activation responses. The large datasets collected from the cell micropatterns facilitate binarization and statistical fitting to calculate a mathematical probability, which assesses the compound’s potential to cause eye irritation. This method potentially enables the amalgamation of multiple mechanistic readouts into a singular index, providing a more accurate and reliable prediction of eye irritation potential in a format amenable to high-throughput screening.