bluuebunny/crossref_metadata_2025 huggingface dataset:
The Improving Speaking Skills through Active Learning Strategy
Authors: Hasan Hasan , Kahar Abd , Hermansyah Sam , Usman Usman
Published: March 2024

This research aimed at proving that active learning strategy can improve the speaking skill of the year eight students of SMP Negeri 1 Sidrap. The researcher used pre-experimental design. The population of this research was the eighth grade students and the sample was class B consisting of 26 students. The sample was chosen by purposive sampling technique. The data were collected through oral test. The pre-test and the post-test were used to measure the improvement of the students‟ speaking skill before and after the treatment. Based on the result of the pre-test and the post-test, it is obtained that the t-counted value is 6.920. By applying 0.05 level of significance and the 25 (26-1) degree of freedom, it was found that t-table value is 2.060. It shows that the t-counted value is greater than the t-table value. It means that the use of active learning strategy can improve speaking skill of the year eight students of SMP Negeri 1 Sidrap.
The Implementation of Communicative Language Teaching (CLT) in Teaching English
Authors: Jabri Umiyati , Samad Ita Sarmita
Published: March 2024

This research was aimed at finding out the way of the teacher in implementing of Communicative Language Teaching (CLT) in teaching English at class of SMPN 1 Enrekang, exploring the media, problems, and types of CLT activities that students prefer.  The population of this research was the teachers and the students of SMPN 1 Enrekang. The sample was three teachers and two classes. In this research, the data were obtained through three kinds of instruments namely, observation checklist, interview, and questionnaire. The result showed the ways of teacher in implementing of CLT were the teachers used principles of CLT methodology stated by Richards (2006:13). Moreover, the researcher found two other ways. Firstly, the teacher always tried to use creative ways/activities in applying Richards’s principles of CLT methodology in order not only to motivate and interest but also to decrease the boredom of students in learning English. Secondly, the teacher showed her sympathy, sensitivity, and understanding to their students’ needs and problems. Generally the problems encountered by the teachers were the students rarely communicated with others using English in daily life so they are lack of English proficiency. The media used by teacher were authentic materials, non-authentic material, listening exercises, internet, and computer based program. The result of questionnaire calculation is about 49.71. This result is in like categories as stated in Liker scale. Based on the data, the top rank of CLT activities that students prefer was task completion activities (100% of students).
Incorporating environmental factors is critical for determining conservation baselines for relative abundance of sharks on coral reefs
Authors: Farabaugh NF , Bond ME , Chapman D , Clua E , Harborne AR , Heupel M , Kiszka JJ , Heithaus MR
Published: March 2024

Populations of sharks, including those inhabiting coral reefs, have experienced dramatic global declines. Setting appropriate targets for restoring reef shark populations requires estimates of expected relative abundances in the absence of intense fishing. It is therefore important to identify factors that drive the carrying capacity of sharks in relatively intact reef systems and to determine whether expected shark abundance varies according to easy to assess variables. These variables could then be used by managers for setting restoration targets, or prioritizing resource allocation, for particular areas in the absence of detailed data. French Polynesia, the world’s largest shark sanctuary, provides a model system for addressing this question. We used baited remote underwater video surveys to assess relative abundance of sharks on 35 reefs across the broad geographic range of French Polynesia. Boosted regression tree models revealed that relative abundance of sharks varied significantly with island geomorphology. Overall, relative shark abundances at high islands were nearly 3 times lower than on atolls, and among atoll geomorphology types, open atolls had abundances nearly 20% higher than closed atolls. Island group, temperature, and net primary productivity had more limited effects. Human pressure (measured as market gravity) was not a significant factor. Although species-specific patterns varied, our findings suggest that environmental factors, particularly island geomorphology, should be taken into account when setting shark abundance recovery targets. Using this easy to assess factor can facilitate the allocation of conservation effort and improve assessments of species recovery efforts for islands in the Indo-Pacific region.
Seasonal variation in marine bird distribution in the northeast Pacific Ocean
Authors: Simon L , Arcese P , Fox CH , Morgan KH , Wilson S
Published: March 2024

Human activities have profound influences on marine ecosystems. Marine birds are particularly sensitive to these impacts and, given their ease of observation and diverse life histories, often represent good indicators of ecosystem health. Conserving marine birds and their ecosystems requires robust predictions of species distribution to help mitigate human disturbance in areas where large aggregations of diverse species occur. We modelled variation in marine bird species diversity (Shannon-Wiener Index) and taxonomic family level probability of occurrence to map the intensity and extent of highly diverse ‘hotspots’ in Canada’s Pacific Exclusive Economic Zone. To do so, we paired 20 yr of survey data from the North Pacific Pelagic Seabird Database (1997-2017) and remote sensing data describing marine conditions and local geography (sea surface temperature, chlorophyll a, bathymetry, distance to shore, and benthic substrate type). These data were used to illustrate how seasonality within years and the El Niño-Southern Oscillation (ENSO) across years influenced spatial patterns in diversity. Hotspots were most persistent in Hecate Strait, off the west coast of Vancouver Island, and surrounding the Scott Islands in most seasons. Changes in hotspot locations and intensity were observed across seasons and within season under varying ENSO conditions. Our results provide a template for mapping marine species distribution for the purpose of identifying hotspots of diversity, and thereby facilitate planning to minimize harmful impacts in highly diverse and dynamic systems.
Elementos para o Ensino de Geografia do Turismo. Programa curricular, orientações didáticas e bibliografia
Authors: Brito-Henriques Eduardo
Published: March 2024

O programa em causa foi concebido e planeado para a licenciatura em Geografia do Instituto de Geografia e Ordenamento do Território da Universidade de Lisboa (IGOT-ULisboa). Há um período inicial de formação de base dedicado às aprendizagens consideradas fundamentais nas três áreas científicas do curso: Geografia Humana, Geografia Física, e Métodos e Técnicas em Geografia. Este período ocupa os primeiros três semestres do ciclo de estudos e é composto totalmente por UC obrigatórias com 6 ECTS. Segue-se depois uma etapa intermédia, que se prolonga pelos quarto e quinto semestres do curso, em que se procura que o grupo de estudantes diversifique e especialize as aprendizagens, explorando temáticas diversas em UC de opção conforme os seus interesses. 70% do tempo de trabalho decorre então em UC opcionais, que o plano de estudos prevê que funcionem em aulas teórico-práticas, também de 6 ECTS. A Geografia do Turismo não consta do elenco de UC obrigatórias, mas pode ter lugar como UC de opção num daqueles dois semestres pré-terminais (a prática tem sido funcionar no quinto semestre), e foi nesse pressuposto que o programa foi desenhado. Acredito que com pequenos ajustamentos para adaptação às formas de organização de ensino, aos regulamentos específicos das instituições, e, eventualmente, aos contextos locais, este programa possa ser aplicado sem grande dificuldade em cursos homólogos de outras universidades. A aplicação em licenciaturas em Turismo, em cujos planos de estudos frequentemente estão previstas UC de Geografia do Turismo, poderá requerer uma adaptação maior, com aliviação dos conteúdos relativos aos aspetos estruturais do turismo e aprofundamento das questões relacionadas com a territorialização do turismo e os impactes do turismo nas comunidades e no ambiente.
CRISPR-HOLMES-based NAD+ detection
Authors: Zhuang Songkuan , Hu Tianshuai , Zhou Hongzhong , He Shiping , Li Jie , Zhang Yuehui , Gu Dayong , Xu Yong , Chen Yijian , Wang Jin
Published: March 2024

Studies have indicated that the intracellular nicotinamide adenine dinucleotide (NAD+) level is associated with the occurrence and development of many diseases. However, traditional nicotinamide adenine dinucleotide (NAD+) detection techniques are time-consuming and may require large and expensive instruments. We recently found that the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a protein can be inactivated by AcrVA5-mediated acetylation and reactivated by CobB, using NAD+ as the co-factor. Therefore, in this study, we created a CRISPR-Cas12a-based one-step HOLMES(NAD+) system for rapid and convenient NAD+ detection with the employment of both acetylated Cas12a and CobB. In HOLMES(NAD+), acetylated Cas12a loses its trans-cleavage activities and can be reactivated by CobB in the presence of NAD+, cutting ssDNA reporters to generate fluorescence signals. HOLMES(NAD+) shows both sensitivity and specificity in NAD+ detection and can be used for quantitative determination of intracellular NAD+ concentrations. Therefore, HOLMES(NAD+) not only provides a convenient and rapid approach for target NAD+ quantitation but also expands the application scenarios of HOLMES to non-nucleic acid detection.
Effects of the prenatal and postnatal nurturing environment on the phenotype and gut microbiota of mice with polycystic ovary syndrome induced by prenatal androgen exposure: a cross-fostering study
Authors: Kusamoto Akari , Harada Miyuki , Minemura Ayaka , Matsumoto Asami , Oka Kentaro , Takahashi Motomichi , Sakaguchi Nanoka , Azhary Jerilee M. K. , Koike Hiroshi , Xu Zixin , Tanaka Tsurugi , Urata Yoko , Kunitomi Chisato , Takahashi Nozomi , Wada-Hiraike Osamu , Hirota Yasushi , Osuga Yutaka
Published: March 2024

The gut microbiome is implicated in the pathogenesis of polycystic ovary syndrome (PCOS), and prenatal androgen exposure is involved in the development of PCOS in later life. Our previous study of a mouse model of PCOS induced by prenatal dihydrotestosterone (DHT) exposure showed that the reproductive phenotype of PCOS appears from puberty, followed by the appearance of the metabolic phenotype after young adulthood, while changes in the gut microbiota was already apparent before puberty. To determine whether the prenatal or postnatal nurturing environment primarily contributes to these changes that characterize prenatally androgenized (PNA) offspring, we used a cross-fostering model to evaluate the effects of changes in the postnatal early-life environment of PNA offspring on the development of PCOS-like phenotypes and alterations in the gut microbiota in later life. Female PNA offspring fostered by normal dams (exposed to an abnormal prenatal environment only, fostered PNA) exhibited less marked PCOS-like phenotypes than PNA offspring, especially with respect to the metabolic phenotype. The gut microbiota of the fostered PNA offspring was similar to that of controls before adolescence, but differences between the fostered PNA and control groups became apparent after young adulthood. In conclusion, both prenatal androgen exposure and the postnatal early-life environment created by the DHT injection of mothers contribute to the development of PCOS-like phenotypes and the alterations in the gut microbiota that characterize PNA offspring. Thus, both the pre- and postnatal environments represent targets for the prevention of PCOS and the associated alteration in the gut microbiota in later life.
Investigation of the leaching behavior of Na and Si in simulated HLW borosilicate glass obtained from the waste of a 1000 MWe class PWR reactor: using the response surface method
Authors: Hosseinpour Khanmiri Mohammad , Yadollahi Ali , Samadfam Mohammad , Sepehrian Hamid , Outokesh Mohammad
Published: March 2024

The immobilization of high-level nuclear waste (HLW) in glass waste matrices provides the key safety function of slowing down radionuclide emissions from an underground disposal site. This study examines the leaching behavior of two major elements, Na and Si, in HLW borosilicate glass simulated from waste of a 1000 MWe class pressurized water reactor (PWR) using response surface methodology and Box-Behnken Design. The design of the experiment was carried out considering three independent variables: the pH of the solution, the contact time, and the leaching temperature, leading to 17 leaching runs performed using the static product consistency test (PCT). The results of statistical analysis (ANOVA: analysis of variance) indicated that the effects of the individual variables and the interactions between them were statistically significant, and the relative consistency of the data further confirmed the model’s applicability. Data obtained from the PCT experiments revealed that the leaching behavior of Na and Si in the evaluated waste glass exhibited similar behavior to previously researched glasses for each condition tested.
Clinical performance of metagenomic next-generation sequencing for diagnosis of invasive fungal disease after hematopoietic cell transplant
Authors: Zhang Xiaoying , Zhang Lingfeng , Li Yun , Wang Na , Zhang Yicheng
Published: March 2024

Background

Timely diagnosis and appropriate antifungal therapy are critical for improving the prognosis of patients with invasive fungal disease (IFD) after hematopoietic stem cell transplantation (HSCT). We evaluated the performance of metagenomic next-generation sequencing (mNGS) and conventional microbiological testing (CMT), as well as the diagnosis, therapeutic management, and outcomes of IFD after HSCT.

Methods

We retrospectively studied 189 patients who underwent HSCT and were considered at risk for IFD. In total, 46 patients with IFD were enrolled in this study. The IFD consensus was followed for classifying IFD incidents.

Results

Forty-six patients were diagnosed with proven/probable (n = 12), possible (n = 27), and undefined (n = 7) IFD. Aspergillus was the most commonly detected fungal genus. Mucormycosis was found in 15 patients; two had Aspergillus, and one had Candida infections. Compared to CMT, mNGS significantly reduced the time required to identify pathogens (P = 0.0016). mNGS had a much higher sensitivity than CMT (84.78% vs. 36.96%; P < 0.0001). A total of 76.09% of patients received antifungal prophylaxis during fungal infections. All Pneumocystis infections occurred later than 100 days after transplantation. Among patients with Pneumocystis infection, 71.43% occurred following sulfonamide withdrawal, and subsequent treatment with sulfonamide alone or in combination with other drugs was effective. Based on the empirical antifungal treatment, the dosages, modes of administration, frequency of administration, or antifungal of 55.26% of the patients were changed according to the mNGS results. The 4-year overall survival rate of patients diagnosed with IFD after transplantation was 71.55% (95% CI, 55.18%–85.82%). Hypoproteinemia and corticosteroid use are independent risk factors for IFD.

Conclusion

mNGS, which has a high sensitivity and a short detection time, aids in the diagnosis and prognosis of pathogenic fungi. As a powerful technology, mNGS can influence treatment decisions in patients with IFD following HSCT.
Gut microbiota and risk of coronary heart disease: a two-sample Mendelian randomization study
Authors: Hu Xiang-zhi , Fu Ling-ling , Ye Bin , Ao Man , Yan Ming , Feng Hong-chao
Published: March 2024

Background

The relationship between gut microbiota composition and coronary heart disease (CHD) has been recently reported in several observational studies. However, the causal effect of gut microbiota on coronary heart disease is uncharted.

Objective

This study attempted to investigate the effect of gut microbiota on coronary heart disease by Mendelian randomization (MR) analysis.

Methods

Through the two-sample MR method, single-nucleotide polymorphisms relevant to gut microbiota were selected as instrument variables to evaluate the causal association between gut microbiota and the risk of CHD.

Results

According to the selection criteria of the inverse variance-weighted average method, Class Actinobacteria, Class Lentisphaeria, Family Clostridiales vadinBB60group, Genus Clostridium innocuum group, Genus Bifidobacterium, Genus Butyricicoccus, Genus Oxalobacter, Genus Turicibacter, and Order Victivallales, presented a suggestive association with coronary heart disease.

Conclusion

This two-sample Mendelian randomization study found that gut microbiota was causally associated with coronary heart disease. Further randomized controlled trials are needed to clarify the protective effect of probiotics on coronary heart disease and their specific protective mechanisms.
Molecular mechanisms of metabolic dysregulation in diabetic cardiomyopathy
Authors: Zeng Yue , Li Yilang , Jiang Wenyue , Hou Ning
Published: March 2024

Diabetic cardiomyopathy (DCM), one of the most serious complications of diabetes mellitus, has become recognized as a cardiometabolic disease. In normoxic conditions, the majority of the ATP production (>95%) required for heart beating comes from mitochondrial oxidative phosphorylation of fatty acids (FAs) and glucose, with the remaining portion coming from a variety of sources, including fructose, lactate, ketone bodies (KB) and branched chain amino acids (BCAA). Increased FA intake and decreased utilization of glucose and lactic acid were observed in the diabetic hearts of animal models and diabetic patients. Moreover, the polyol pathway is activated, and fructose metabolism is enhanced. The use of ketones as energy sources in human diabetic hearts also increases significantly. Furthermore, elevated BCAA levels and impaired BCAA metabolism were observed in the hearts of diabetic mice and patients. The shift in energy substrate preference in diabetic hearts results in increased oxygen consumption and impaired oxidative phosphorylation, leading to diabetic cardiomyopathy. However, the precise mechanisms by which impaired myocardial metabolic alterations result in diabetes mellitus cardiac disease are not fully understood. Therefore, this review focuses on the molecular mechanisms involved in alterations of myocardial energy metabolism. It not only adds more molecular targets for the diagnosis and treatment, but also provides an experimental foundation for screening novel therapeutic agents for diabetic cardiomyopathy.
Hair cortisol and changes in cortisol dynamics in chronic kidney disease
Authors: Boswell Laura , Vega-Beyhart Arturo , Blasco Miquel , Quintana Luis F. , Rodríguez Gabriela , Díaz-Catalán Daniela , Vilardell Carme , Claro María , Mora Mireia , Amor Antonio J. , Casals Gregori , Hanzu Felicia A.
Published: March 2024

Objective

We compared hair cortisol (HC) with classic tests of the hypothalamic–pituitary–adrenal (HPA) axis in chronic kidney disease (CKD) and assessed its association with kidney and cardiometabolic status.

Design and methods

A cross-sectional study of 48 patients with CKD stages I–IV, matched by age, sex, and BMI with 24 healthy controls (CTR) was performed. Metabolic comorbidities, body composition, and HPA axis function were studied.

Results

A total of 72 subjects (age 52.9 ± 12.2 years, 50% women, BMI 26.2 ± 4.1 kg/m2) were included. Metabolic syndrome features (hypertension, dyslipidaemia, glucose, HOMA-IR, triglycerides, waist circumference) and 24-h urinary proteins increased progressively with worsening kidney function (p < 0.05 for all). Reduced cortisol suppression after 1-mg dexamethasone suppression (DST) (p < 0.001), a higher noon (12:00 h pm) salivary cortisol (p = 0.042), and salivary cortisol AUC (p = 0.008) were seen in CKD. 24-h urinary-free cortisol (24-h UFC) decreased in CKD stages III–IV compared with I–II (p < 0.001); higher midnight salivary cortisol (p = 0.015) and lower suppressibility after 1-mg DST were observed with declining kidney function (p < 0.001). Cortisol-after-DST cortisol was >2 mcg/dL in 23% of CKD patients (12.5% in stage III and 56.3% in stage IV); 45% of them had cortisol >2 mcg/dL after low-dose 2-day DST, all in stage IV (p < 0.001 for all). Cortisol-after-DST was lineally inversely correlated with eGFR (p < 0.001). Cortisol-after-DST (OR 14.9, 95% CI 1.7–103, p = 0.015) and glucose (OR 1.3, 95% CI 1.1–1.5, p = 0.003) were independently associated with eGFR <30 mL/min/m2). HC was independently correlated with visceral adipose tissue (VAT) (p = 0.016). Cortisol-after-DST (p = 0.032) and VAT (p < 0.001) were independently correlated with BMI.

Conclusion

Cortisol-after-DST and salivary cortisol rhythm present progressive alterations in CKD patients. Changes in cortisol excretion and HPA dynamics in CKD are not accompanied by significant changes in long-term exposure to cortisol evaluated by HC. The clinical significance and pathophysiological mechanisms explaining the associations between HPA parameters, body composition, and kidney damage warrant further study.
Association between napping and type 2 diabetes mellitus
Authors: Liu Hongyi , Wu Yingxin , Zhu Hui , Wang Penghao , Chen Tao , Xia Anyu , Zhao Zhijia , He Da , Chen Xiang , Xu Jin , Ji Lindan
Published: March 2024

As the incidence of type 2 diabetes mellitus (T2DM) is increasing rapidly and its consequences are severe, effective intervention and prevention, including sleep-related interventions, are urgently needed. As a component of sleep architecture, naps, alone or in combination with nocturnal sleep, may influence the onset and progression of T2DM. Overall, napping is associated with an increased risk of T2DM in women, especially in postmenopausal White women. Our study showed that napping >30 minutes (min) increased the risk of T2DM by 8-21%. In addition, non-optimal nighttime sleep increases T2DM risk, and this effect combines with the effect of napping. For nondiabetic patients, napping >30 min could increase the risks of high HbA1c levels and impaired fasting glucose (IFG), which would increase the risk of developing T2DM later on. For diabetic patients, prolonged napping may further impair glycemic control and increase the risk of developing diabetic complications (e.g., diabetic nephropathy) in the distant future. The following three mechanisms are suggested as interpretations for the association between napping and T2DM. First, napping >30 min increases the levels of important inflammatory factors, including interleukin 6 and C-reactive protein, elevating the risks of inflammation, associated adiposity and T2DM. Second, the interaction between postmenopausal hormonal changes and napping further increases insulin resistance. Third, prolonged napping may also affect melatonin secretion by interfering with nighttime sleep, leading to circadian rhythm disruption and further increasing the risk of T2DM. This review summarizes the existing evidence on the effect of napping on T2DM and provides detailed information for future T2DM intervention and prevention strategies that address napping.
Lean resource management and reliable interaction for a low-carbon-oriented new grid
Authors: Liu Yuting
Published: March 2024

The lean resource management and reliable interaction of massive data are important components of a low-carbon-oriented new grid. However, with a high proportion of distributed low-carbon resources connected to a new grid, issues such as data anomalies, data redundancy, and missing data lead to inefficient resource management and unreliable interaction, affecting the accuracy of power grid decision-making, as well as the effectiveness of emission reduction and carbon reduction. Therefore, this paper proposes a lean resource management and reliable interaction framework of a middle platform based on distributed data governance. On this basis, a distributed data governance approach for the lean resource management method of the middle platform in the low-carbon new grid is proposed, which realizes anomalous data cleaning and missing data filling. Then, a data storage and traceability method for reliable interaction is proposed, which prevents important data from being illegally tampered with in the interaction process. The simulation results demonstrate that the proposed algorithm significantly enhances efficiency, reliability, and accuracy in anomalous data cleaning and filling, as well as data traceability.
Spatial dynamic simulation of beetles in biodiversity hotspots
Authors: He Ping , Bai Ming , Li Lulu , Lu Yuanyuan , Li Jing , Yan Zihan
Published: March 2024

Introduction

Coleoptera is the most species-rich order of animals with the widest distribution area; however, little is known about its global suitability distribution, and a substantial number of species are experiencing silent extinction. Most of Earth’s biocommunities are concentrated in biodiversity hotspots, and these hotspots receive the largest investment of conservation funds. The survival of beetles is closely related to the richness of biodiversity, so habitat loss in hotspots results in a high extinction risk for beetles.

Methods

We used the MaxEnt model to simulate the spatial suitable distribution in six time periods (1970-1980, 1980-1990, 1990-2000, 2000-2010, 2010-2020, and 1970-2020). Furthermore, we determined the priority suitable regions in hotspots after coupling the current biodiversity zones and evaluated the congruence between the suitable habitat of beetles and hotspots.

Results

The results revealed that less than 30% of suitable regions were located in hotspots, and approximately 49.08% of the suitable habitat remained constant in all six periods, and can be regarded as climatically stable refugia. The Mediterranean Basin had the largest suitable area in each period and was always ranked first; other regions, such as Indo-Burma, Irano-Anatolian, and Mesoamerica, also had large areas of suitable habitat. Nine excellent, ten stable, and seventeen lower hotspots were identified based on their dynamic changes in 36 hotspots.

Discussion

Based on these results, corresponding planning and management measures should be implemented in different biodiversity hotspots according to the changes in geographic distribution. In this paper, we propose practical advice for different regions to improve isolated and fragmented habitats, which will support the conservation of Coleoptera taxa and the restoration of biodiversity.
Is the exquisite specificity of lymphocytes generated by thymic selection or due to evolution?
Authors: De Boer Rob J. , Kesmir Can , Perelson Alan S. , Borghans José A. M.
Published: March 2024

We have previously argued that the antigen receptors of T and B lymphocytes evolved to be sufficiently specific to avoid massive deletion of clonotypes by negative selection. Their optimal ‘specificity’ level, i.e., probability of binding any particular epitope, was shown to be inversely related to the number of self-antigens that the cells have to be tolerant to. Experiments have demonstrated that T lymphocytes also become more specific during negative selection in the thymus, because cells expressing the most crossreactive receptors have the highest likelihood of binding a self-antigen, and hence to be tolerized (i.e., deleted, anergized, or diverted into a regulatory T cell phenotype). Thus, there are two —not mutually exclusive— explanations for the exquisite specificity of T cells, one involving evolution and the other thymic selection. To better understand the impact of both, we extend a previously developed mathematical model by allowing for T cells with very different binding probabilities in the pre-selection repertoire. We confirm that negative selection tends to tolerize the most crossreactive clonotypes. As a result, the average level of specificity in the functional post-selection repertoire depends on the number of self-antigens, even if there is no evolutionary optimization of binding probabilities. However, the evolutionary optimal range of binding probabilities in the pre-selection repertoire also depends on the number of self-antigens. Species with more self antigens need more specific pre-selection repertoires to avoid excessive loss of T cells during thymic selection, and hence mount protective immune responses. We conclude that both evolution and negative selection are responsible for the high level of specificity of lymphocytes.
Polygenic risk associated with Alzheimer’s disease and other traits influences genes involved in T cell signaling and activation
Authors: Dressman Dallin , Tasaki Shinya , Yu Lei , Schneider Julie , Bennett David A. , Elyaman Wassim , Vardarajan Badri
Published: March 2024

Introduction

T cells, known for their ability to respond to an enormous variety of pathogens and other insults, are increasingly recognized as important mediators of pathology in neurodegeneration and other diseases. T cell gene expression phenotypes can be regulated by disease-associated genetic variants. Many complex diseases are better represented by polygenic risk than by individual variants.

Methods

We first compute a polygenic risk score (PRS) for Alzheimer’s disease (AD) using genomic sequencing data from a cohort of Alzheimer’s disease (AD) patients and age-matched controls, and validate the AD PRS against clinical metrics in our cohort. We then calculate the PRS for several autoimmune disease, neurological disorder, and immune function traits, and correlate these PRSs with T cell gene expression data from our cohort. We compare PRS-associated genes across traits and four T cell subtypes.

Results

Several genes and biological pathways associated with the PRS for these traits relate to key T cell functions. The PRS-associated gene signature generally correlates positively for traits within a particular category (autoimmune disease, neurological disease, immune function) with the exception of stroke. The trait-associated gene expression signature for autoimmune disease traits was polarized towards CD4+ T cell subtypes.

Discussion

Our findings show that polygenic risk for complex disease and immune function traits can have varying effects on T cell gene expression trends. Several PRS-associated genes are potential candidates for therapeutic modulation in T cells, and could be tested in in vitro applications using cells from patients bearing high or low polygenic risk for AD or other conditions.
Uncovering the role of oxygen on organic carbon cycling: insights from a continuous culture study with a facultative anaerobic bacterioplankton species (Shewanella baltica)
Authors: Maßmig Marie , Cisternas-Novoa Carolina , Engel Anja
Published: March 2024

Deoxygenation is tied to organic carbon (Corg) supply and utilization in marine systems. Under oxygen-depletion, bacteria maintain Corg respiration using alternative electron acceptors such as nitrate. Since anaerobic respiration’s energy yield is lower, Corg remineralization may be reduced and its residence time increased. We investigated the influence of oxygen and alternative electron acceptors’ availability on Corg cycling by heterotrophic bacteria during a continuous culture experiment with Shewanella baltica, a facultative anaerobic γ-Proteobacteria in the Baltic Sea. We tested six different oxygen levels, from suboxic (<5 µmol L-1) to fully oxic conditions, using a brackish (salinity=14 g L-1) media supplied with high (HighN) or low (LowN) inorganic nitrogen concentrations relative to glucose as labile Corg source. Our results show that suboxia limited DOC (glucose) uptake and cell growth only under LowN, while higher availability of alternative electron acceptors seemingly compensated oxygen limitation under HighN. N-loss was observed under suboxia in both nitrogen treatments. Under HighN, N-loss was highest and a C:N loss ratio of ~2.0 indicated that Corg was remineralized via denitrification. Under LowN, the C:N loss ratio under suboxia was higher (~5.5), suggesting the dominance of other anaerobic respiration pathways, such as dissimilatory nitrate reduction to ammonium (DNRA). Bacterial growth efficiency was independent of oxygen concentration but higher under LowN (34 ± 3.0%) than HighN (26 ± 1.6%). Oxygen concentration also affected dissolved organic matter (DOM) cycling. Under oxic conditions, the release of dissolved combined carbohydrates was enhanced, and the amino acid-based degradation index (DI) pointed to more diagenetically altered DOM. Our results suggest bacterial Corg uptake in low-oxygen systems dominated by S. baltica can be limited by oxygen but compensated by high nitrate availability. Hence, suboxia diminishes Corg remineralisation only when alternative electron acceptors are lacking. Under high nitrate:Corg supply, denitrification leads to a higher N:C loss ratio, potentially counteracting eutrophication in the long run. Low nitrate:Corg supply may favour other anaerobic respiration pathways like DNRA, which sustains labile nitrogen in the system, potentially intensifying the cycle of eutrophication. Going forward, it will be crucial to establish the validity of our findings for S. baltica in natural systems with diverse organic substrates and microbial consortia.
Multi-object detection and behavior tracking of sea cucumbers with skin ulceration syndrome based on deep learning
Authors: Ge Fengli , Xuan Kui , Lou Peng , Li Juan , Jiang Lingxu , Wang Jiasheng , Lin Qi
Published: March 2024

Skin ulceration syndrome of sea cucumbers is one of the most serious diseases in intensive aquaculture, and it is the most effective way of preventing the spread of this disease to detect the abnormal behavior of sea cucumbers in time and take corresponding measures. However, the detection and tracking of multi-object is a hard problem in sea cucumber behavior analysis. To solve this problem, this paper first proposes a novel one-stage algorithm SUS-YOLOv5 for multi-object detection and tracking of sea cucumbers. The proposed SUS-YOLOv5 optimizes the maximum suppression algorithm in the overlapping region of the object detection box. Next, the SE-BiFPN feature fusion structure is proposed to enhance the transmission efficiency of feature information between deep and shallow layers of the network. Then, a MO-Tracking algorithm is proposed integrated with DeepSORT to achieve real-time multi-object tracking. Experimental results show that the mAP@0.5 and mAP@0.5:0.95 of the proposed object detector reach 95.40% and 83.80%, respectively, which are 3.30% and 4.10% higher than the original YOLOv5s. Compared with the traditional SSD, YOLOv3, and YOLOv4, the mAP of SUS-YOLOv5 is improved by 5.49%, 1.57%, and 3.76%, respectively. This research can realize the multi-object detection and tracking, which lays the foundation for the prediction of skin ulceration syndrome in sea cucumbers and has a certain practical application value for improving the intelligence level of aquaculture.
Short-term lifestyle education on obesity reduction in adolescents
Authors: Ning Feng , Sun Xiaohui , Ge Bing , Li Shunping , Hou Binghui , Wang Yumei , Zhang Dong
Published: March 2024

Backgrounds

Obesity is increasing in adolescents in China. However, the awareness of obesity and prevention on related risk factors were not well known. We aim to assess the effectiveness of short-term health education intervention on obesity in Chinese adolescents.

Methods

In this study, 42 primary and secondary schools from Qingdao were randomly divided into the education and control groups. A total of 11,739 adolescents was included in the current study. The logistic regression was employed to assess odds ratio (OR) of education intervention on overweight and obesity prevalence adjusting for covariates.

Results

The baseline prevalence of overweight and obesity was significantly higher in urban than in rural areas and in boys than in girls. After 1 year lifestyle intervention, the proportion of students with awareness of obesity was higher, meanwhile age-adjusted mean values of weight, body mass index, duration of watching TV and doing homework were lower in education group than control group. The corresponding figures were 43.6 [95% CI (confidence intervals); 43.3–43.9] kg versus 44.3 (95% CI; 44.0–44.6) kg, 18.6 (95% CI; 18.5–18.7) kg/m2 versus 18.9 (95% CI; 18.8–19.1) kg/m2, 1.3 (95% CI; 1.2–1.3) hours/d versus 1.4 (95% CI; 1.3–1.4) hours/d, and 1.5 (95% CI; 1.4–1.5) hours/d versus 1.8 (95% CI, 1.7–1.8) hours/d. The multivariable adjusted OR for combined prevalence of overweight and obesity was 0.85 (95% CI, 0.76–0.96) in education group as compared with control group.

Conclusion

Short-term health education intervention results in significantly higher reductions in obesity parameters and improvement in awareness in Chinese adolescents.
Transcriptome analysis of Gossypium reveals the molecular mechanisms of Ca2+ signaling pathway on arsenic tolerance induced by arbuscular mycorrhizal fungi
Authors: Gong Minggui , Bai Na , Su Jiajie , Wang Yuan , Wei Yanan , Zhang Qiaoming
Published: March 2024

Introduction

Arbuscular mycorrhizal fungi (AMF) have been demonstrated their ability to enhance the arsenic (As) tolerance of host plants, and making the utilization of mycorrhizal plants a promising and practical approach for remediating As-contaminated soils. However, comprehensive transcriptome analysis to reveal the molecular mechanism of As tolerance in the symbiotic process between AMF and host plants is still limited.

Methods

In this study, transcriptomic analysis of Gossypium seedlings was conducted with four treatments: non-inoculated Gossypium under non-As stress (CK0), non-inoculated Gossypium under As stress (CK100), F. mosseae-inoculated Gossypium under non-As stress (FM0), and F. mosseae-inoculated Gossypium under As stress (FM100).

Results

Our results showed that inoculation with F. mosseae led to a reduction in net fluxes of Ca2+, while increasing Ca2+ contents in the roots and leaves of Gossypium under the same As level in soil. Notably, 199 and 3129 differentially expressed genes (DEGs) were specially regulated by F. mosseae inoculation under As stress and non-As stress, respectively. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation and enrichment analyses, we found that under As stress, F. mosseae inoculation up-regulated a significant number of genes related to the Ca2+ signaling pathway genes, involved in cellular process, membrane part, and signal transduction. This suggests a potential role in mitigating As tolerance in Gossypium seedlings. Furthermore, our analysis identified specific DEGs in transcription factor families, including ERF, MYB, NAC, and WRKY, that were upregulated by F. mosseae inoculation. Conversely, MYB and HB-other were down-regulated. The ERF and MYB families exhibited the highest number of up- and down-regulated DEGs, respectively, which were speculated to play an important role in alleviating the As toxicity of Gossypium.

Discussion

Our findings provided valuable insights into the molecular theoretical basis of the Ca2+ signaling pathway in improving As tolerance of mycorrhizal plants in the future.
A systematic review of the clinical evidence for an association between type I hypersensitivity and inner ear disorders
Authors: Zeng Bin , Domarecka Ewa , Kong Lingyi , Olze Heidi , Scheffel Jörg , Moñino-Romero Sherezade , Siebenhaar Frank , Szczepek Agnieszka J.
Published: March 2024

Inner ear disorders have a variety of causes, and many factors can contribute to the exacerbation of cochlear and vestibular pathology. This systematic review aimed to analyze clinical data on the coexistence and potential causal interaction between allergic diseases and inner ear conditions. A search of PubMed and Web of Science identified 724 articles, of which 21 were selected for full-text analysis based on inclusion and exclusion criteria. The epidemiologic evidence found overwhelmingly supports an association between allergic disease and particular inner ear disorders represented by a high prevalence of allergic reactions in some patients with Ménière’s disease (MD), idiopathic sudden sensorineural hearing loss (ISSHL), and acute low-tone hearing loss (ALHL). In addition, patients with MD, ISSHL, and ALHL had higher levels of total serum IgE than healthy subjects. Finally, in some cases, changes in cochlear potential may have been induced by antigen exposure, while desensitization alleviated allergy and inner ear-related symptoms. The exact mechanism of interaction between the auditory/vestibular and immune systems is not fully understood, and further clinical and basic research is needed to understand the relationship between the two systems fully.
Rapid assessment of peripheral visual crowding
Authors: Tanriverdi Dilce , Cornelissen Frans W.
Published: March 2024

Visual crowding, the phenomenon in which the ability to distinguish objects is hindered in cluttered environments, has critical implications for various ophthalmic and neurological disorders. Traditional methods for assessing crowding involve time-consuming and attention-demanding psychophysical tasks, making routine examination challenging. This study sought to compare trial-based Alternative Forced-Choice (AFC) paradigms using either manual or eye movement responses and a continuous serial search paradigm employing eye movement responses to evaluate their efficiency in rapidly assessing peripheral crowding. In all paradigms, we manipulated the orientation of a central Gabor patch, which could be presented alone or surrounded by six Gabor patches. We measured participants’ target orientation discrimination thresholds using adaptive psychophysics to assess crowding magnitude. Depending on the paradigm, participants either made saccadic eye movements to the target location or responded manually by pressing a key or moving a mouse. We compared these paradigms in terms of crowding magnitude, assessment time, and paradigm demand. Our results indicate that employing eye movement-based paradigms for assessing peripheral visual crowding yields results faster compared to paradigms that necessitate manual responses. Furthermore, when considering similar levels of confidence in the threshold measurements, both a novel serial search paradigm and an eye movement-based 6AFC paradigm proved to be the most efficient in assessing crowding magnitude. Additionally, crowding estimates obtained through either the continuous serial search or the 6AFC paradigms were consistently higher than those obtained using the 2AFC paradigms. Lastly, participants did not report a clear difference between paradigms in terms of their perceived demand. In conclusion, both the continuous serial search and the 6AFC eye movement response paradigms enable a fast assessment of visual crowding. These approaches may potentially facilitate future routine crowding assessment. However, the usability of these paradigms in specific patient populations and specific purposes should be assessed.
Reduction in systemic muscle stress markers after exercise-induced muscle damage following concurrent training and supplementation with specific collagen peptides – a randomized controlled trial
Authors: Bischof Kevin , Stafilidis Savvas , Bundschuh Larissa , Oesser Steffen , Baca Arnold , König Daniel
Published: March 2024

Introduction

Collagen peptide supplementation in conjunction with exercise has been shown to improve structural and functional adaptations of both muscles and the extracellular matrix. This study aimed to explore whether specific collagen peptide (SCP) supplementation combined with a concurrent training intervention can improve muscular stress after exercise-induced muscle damage, verified by reliable blood markers.

Methods

55 sedentary to moderately active males participating in a concurrent training (CT) intervention (3x/week) for 12 weeks were administered either 15 g of SCP or placebo (PLA) daily. Before (T1) and after the intervention (T2), 150 muscle-damaging drop jumps were performed. Blood samples were collected to measure creatine kinase (CK), lactate dehydrogenase (LDH), myoglobin (MYO) and high-sensitivity C-reactive protein (hsCRP) before, after, and at 2 h, 24 h and 48 h post exercise.

Results

A combination of concurrent training and SCP administration showed statistically significant interaction effects, implying a lower increase in the area under the curve (AUC) of MYO (p = 0.004, ηp2 = 0.184), CK (p = 0.01, ηp2 = 0.145) and LDH (p = 0.016, ηp2 = 0.133) in the SCP group. On closer examination, the absolute mean differences (ΔAUCs) showed statistical significance in MYO (p = 0.017, d = 0.771), CK (p = 0.039, d = 0.633) and LDH (p = 0.016, d = 0.764) by SCP supplementation.

Conclusion

In conclusion, 12 weeks of 15 g SCP supplementation combined with CT intervention reduced acute markers of exercise-induced muscle damage and improved post-exercise regenerative capacity, as evidenced by the altered post-exercise time course. The current findings indicate that SCP supplementation had a positive effect on the early phase of muscular recovery by either improving the structural integrity of the muscle and extracellular matrix during the training period or by accelerating membrane and cytoskeletal protein repair.

Clinical trial registration

https://www.clinicaltrials.gov/study/NCT05220371?cond=NCT05220371&rank=1, NCT05220371.
Unveiling the immunosuppressive landscape of pancreatic ductal adenocarcinoma: implications for innovative immunotherapy strategies
Authors: Guo Songyu , Wang Zhenxia
Published: March 2024

Pancreatic cancer, particularly pancreatic ductal adenocarcinoma (PDAC), stands as the fourth leading cause of cancer-related deaths in the United States, marked by challenging treatment and dismal prognoses. As immunotherapy emerges as a promising avenue for mitigating PDAC’s malignant progression, a comprehensive understanding of the tumor’s immunosuppressive characteristics becomes imperative. This paper systematically delves into the intricate immunosuppressive network within PDAC, spotlighting the significant crosstalk between immunosuppressive cells and factors in the hypoxic acidic pancreatic tumor microenvironment. By elucidating these mechanisms, we aim to provide insights into potential immunotherapy strategies and treatment targets, laying the groundwork for future studies on PDAC immunosuppression. Recognizing the profound impact of immunosuppression on PDAC invasion and metastasis, this discussion aims to catalyze the development of more effective and targeted immunotherapies for PDAC patients.