bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance Test and Structure Optimization of a Marine Diesel Particulate Filter
Authors: Yang Zhiyuan , Chen Haowen , Li Changxiong , Guo Hao , Tan Qinming
Published: May 2023

Particulate matter (PM) is a major pollutant in the exhaust of marine diesel engines, which seriously endangers human health and the atmospheric environment, and how to reduce particulate matter emissions from marine engines has become a key research direction in the field of environmental protection and diesel engines. In this study, we analyzed the components and sources of PM from marine engines and conducted tests on the performance of Wärtsilä 20DF Diesel Particulate Filter (DPF) catalysts to verify the capture efficiency, gaseous pollutant removal rate, regeneration effect and the relationship between carbon loading and pressure loss of DPF catalysts in the context of Tier III emission regulations. The results showed that PM emissions of 20DF in diesel mode after adding the DPF system meet the requirements of the regulatory limit, but the pressure drop of the engine increases after adding the DPF system. Therefore, numerical simulation was used to optimize the DPF structure by evaluating the system velocity field, flow field distribution uniformity and system pressure drop to improve the pressure drop.
Comparative Assessment of sCO2 Cycles, Optimal ORC, and Thermoelectric Generators for Exhaust Waste Heat Recovery Applications from Heavy-Duty Diesel Engines
Authors: Ahamed Menaz , Pesyridis Apostolos , Ahbabi Saray Jabraeil , Mahmoudzadeh Andwari Amin , Gharehghani Ayat , Rajoo Srithar
Published: May 2023

This study aimed to investigate the potential of supercritical carbon dioxide (sCO2), organic Rankine cycle (ORC), and thermoelectric generator (TEG) systems for application in automotive exhaust waste heat recovery (WHR) applications. More specifically, this paper focuses on heavy-duty diesel engines applications such as marine, trucks, and locomotives. The results of the simulations show that sCO2 systems are capable of recovering the highest amount of power from exhaust gases, followed by ORC systems. The sCO2 system recovered 19.5 kW at the point of maximum brake power and 10.1 kW at the point of maximum torque. Similarly, the ORC system recovered 14.7 kW at the point of maximum brake power and 7.9 kW at the point of maximum torque. Furthermore, at a point of low power and torque, the sCO2 system recovered 4.2 kW of power and the ORC system recovered 3.3 kW. The TEG system produced significantly less power (533 W at maximum brake power, 126 W at maximum torque, and 7 W at low power and torque) at all three points of interest due to the low system efficiency in comparison to sCO2 and ORC systems. From the results, it can be concluded that sCO2 and ORC systems have the biggest potential impact in exhaust WHR applications provided the availability of heat and that their level of complexity does not become prohibitive.
Experimental Analysis of Oscillatory Vortex Generators in Wind Turbine Blade
Authors: Parra Hector G. , Ceron Hernan D. , Gomez William , Gaona Elvis E.
Published: May 2023

Vortex generators are devices that modify the wind behavior near the surface of wind turbine blades. Their use allows the boundary layer shedding transition zone to be varied. Bio-inspired design has been used to improve the efficiency of aerodynamic and hydrodynamic systems by creating devices that use shapes present in animals and plants. In this work, an experimental methodology is proposed to study the effect of bio-inspired vortex generators and their effect on the structural vibration of a blade. In addition, the wind wake generated by the blade with oscillating vortex generators at different oscillation frequencies is analyzed by means of a hot wire anemometer, obtaining appreciable vibration reduction results in the measured 3D acceleration signals for wind velocities between 10 and 15 m/s. Values of the spectral components of the wake velocity measured at higher tunnel wind velocities increase. Spectral variance is reduced at higher tunnel wind velocities. The system analyzed in this paper can contribute in the future to the construction of actuators for vibration compensation systems in wind turbines.
Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast Yarrowia lipolytica
Authors: Liu Mengchan , Cheng Hairong
Published: May 2023

Yarrowia lipolytica, a GRAS (generally recognized as safe) nonconventional yeast, has been used widely in industrial fermentation to produce chemicals, fuels, and functional sugars such as erythritol and mannitol. Although Y. lipolytica is a promising organism for bioconversion and has substantial potential in industrial production, its utilization is restricted by the high cost of cooling during the fermentation process; the optimum growth or fermentation temperature of Y. lipolytica is 28–30 °C, which is lower than that of some fermenting species. Therefore, it is necessary to breed a thermoresistant Y. lipolytica for use in a fermentation system. Here, we report a new thermoduric Y. lipolytica strain (a thermoresistant clone, HRC) that can grow at 35 °C—higher than the starting strain Y. lipolytica CGMCC7326 (maximum growth temperature at 33 °C)—by laboratory adaptive evolution. Based on the transcriptome analysis of the mutant strain HRC and the parental strain Y. lipolytica CGMCC7326 at different temperatures, 22 genes with increased expression at high temperatures were identified and 10 of them were overexpressed in Y. lipolytica CGMCC7326. HRC1, HRC2, and HRC3 (with YALI0B21582g, YALI0C13750g, and YALI0B10626g overexpressed, respectively) were assessed for growth at higher temperatures. This revealed that these three genes were related to thermotolerance. This study provides insights into the metabolic landscape of Y. lipolytica under heat stress, enabling future metabolic engineering endeavors to improve both thermoresistance and sugar alcohol production in the yeast Y. lipolytica.
Heterologous Biosynthesis of Hyaluronic Acid Using a New Hyaluronic Acid Synthase Derived from the Probiotic Streptococcus thermophilus
Authors: Zhong Qian , Ma Yanqin , Xu Delei , Lei Peng , Li Sha , Xu Hong , Qiu Yibin
Published: May 2023

Hyaluronic acid (HA) is a natural linear polysaccharide extensively used in many fields, including the food, medicine, and cosmetics industries. Currently, species that produce HA synthetase (HAS) from microbial sources are relatively small and mainly pathogenic, such as Streptococcus pyogenes and Pasteurella multicide. Moreover, there is limited research on the safe microbial sources of HAS. Thus, we characterized SthasA, a HAS derived from the probiotic Streptococcus thermophilus, and used it for the de novo synthesis of HA in a chassis strain of Bacillus amyloliquefaciens. Metabolic engineering of the precursor supply modules suggested that hasB (encoding UDPG dehydrogenase), which was derived from Corynebacterium glutamicum ATCC 13032, effectively promoted the accumulation of HA products. Furthermore, by combining the expression of the global regulatory factor CcpA, HA yield from the recombinant strain reached 3.20 g/L. Finally, we obtained a yield of 5.57 g/L HA with a molecular weight of 1.7 × 106 Da using various process optimization strategies in a 5 L bioreactor. This study enriches our understanding of obtaining HAS from non-pathogenic bacteria and provides a safe and effective process for producing HA, which has the potential to promote the industrial applications of HA further.
The Effect of Lactobacillus planturum YQM48 Inoculation on the Quality and Microbial Community Structure of Alfalfa Silage Cultured in Saline-Alkali Soil
Authors: Liu Yinghao , Wang Yongjie , Zhang Lianyi , Liu Ling , Cai Ting , Chang Chun , Sa Duowen , Yin Qiang , Jiang Xiaowei , Li Yuyu , Lu Qiang
Published: May 2023

Alfalfa cultivated in salt–alkali soil was used for fermentation, to which an inoculum of Lactobacillus plantarum YQM48 was added, to assess its effect on the feed quality and the microbial community structure of the fermented silage. A control was included without inoculum. The nutritional components, fermentation quality, pH, and microbial community of the silage were measured after 30 and 60 days of anaerobic fermentation. The results showed that after 30 and 60 days of fermentation in the presence of the inoculum, the content of water-soluble carbohydrates, crude protein content, and dry matter were all significantly higher than those of the control silage, the pH and butyric acid content were lower, and the content of lactic acid and acetic acid were higher (p < 0.05 for all). There was no significant difference in pH and butyric acid content between 30 and 60 days of fermentation in the presence of the inoculum (p > 0.05), while the lactic acid and acetic acid contents were significantly lower in the 60-day silage compared to the 30-day silage (p < 0.05). Fermentation reduced the abundance of Cyanobacteria and Proteobacteria (the abundant phyla in the fresh alfalfa), while the abundance of Firmicutes increased, reaching 92.3% after 60 days of fermentation with inoculum. The dominant genus in that sample was Lactobacillus (70.0%), followed by Enterococcus (12.9%), while fermentation of 60 days without inoculum resulted in only 29.2% Lactobacillus, together with 27.8% Cyanobacteria, and 12.2% Enterococcus. In summary, the addition of L. plantarum YQM48 can improve the nutritional components and fermentation quality of alfalfa silage cultivated on a salt-alkali soil.
Vermi-Conversion of Anaerobic Sludges by Eisenia fetida Earthworms
Authors: Vavouraki Aikaterini Ioannis , Kornaros Michael
Published: May 2023

Sludge management is considered a difficult and challenging task and is a priority of environmental policy. This study evaluates the transformation of the anaerobic sludge of agroindustrial wastes (cow manure, anaerobic sludge mixtures) directly to compost using Eisenia fetida earthworms (vermicomposting) in relation to sludge quality; moreover, it investigates the effects of different sludge compositions on vermicompost physicochemical properties. In particular, the biostabilization of anaerobic sludge (AS1) produced in excess from the wastewater treatment plant (WWTP) of Patras, Greece, and mixed anaerobic sludge (AS2) originating from the effluents of a laboratory anaerobic co-digestion system treating an agroindustrial waste mixture (olive mill wastewater, cheese whey, and liquid cow manure in a ratio of 55: 40: 5 (w/w), respectively) mixed with cow dung (CD) using Eisenia fetida earthworms was examined. Comparing the mixtures of CD-AS1 to CD-AS2, superior results were obtained with the use of AS2 since an increase in N-P-K was observed when either 10% (22%, 51.8%, and 2.4%, respectively) or 15% of AS2 (38.7%, 14.1%, and 8.1% respectively) was used. Although a reduction in earthworms’ growth was observed compared to 100% CD, during the vermicomposting of the CD-AS mixtures, 410 and 250 mg/earthworm was sustained in the mixtures of 85% CD-15% AS2 and 80% CD-20% AS2 after a period of 63 and 70 days of vermicomposting, respectively.
Influence of Cmr1 in the Regulation of Antioxidant Function Melanin Biosynthesis in Aureobasidium pullulans
Authors: Wang Wan , Zhang Kai , Lin Congyu , Zhao Shanshan , Guan Jiaqi , Zhou Wei , Ru Xin , Cong Hua , Yang Qian
Published: May 2023

We have successfully identified the transcription factor Cmr1 from the fungus Aureobasidium pullulans Hit-lcy3T, which regulates melanin biosynthesis genes. Bioinformatics analysis revealed that the Cmr1 gene encodes a protein of 945 amino acids, containing two Cys2His2 zinc finger domains and a Zn(II)2Cys6 binuclear cluster domain located at the N-terminus of Cmr1. To investigate the function of the Cmr1 gene, we performed gene knockout and overexpression experiments. Our results showed that Cmr1 is a key regulator of melanin synthesis in Hit-lcy3T, and its absence caused developmental defects. Conversely, overexpression of Cmr1 significantly increased the number of chlamydospores in Hit-lcy3T and improved melanin production. RT-qPCR analysis further revealed that overexpression of Cmr1 enhanced the expression of several genes involved in melanin biosynthesis, including Cmr1, PKS, SCD1, and THR1. Melanin extracted from the Hit-lcy3T was characterized using UV and IR spectroscopy. Furthermore, we assessed the antioxidant properties of Hit-lcy3T melanin and found that it possesses strong scavenging activity against DPPH·, ABTS·, and OH·, but weaker activity against O2−·. These findings suggest that Hit-lcy3T melanin holds promise for future development as a functional food additive.
Analysis of a Fractional-Order Model for African Swine Fever with Effect of Limited Medical Resources
Authors: Shi Ruiqing , Li Yang , Wang Cuihong
Published: May 2023

In this paper, a fractional-order model for African swine fever with limited medical resources is proposed and analyzed. First, the existence and uniqueness of a positive solution is proven. Second, the basic reproduction number and the conditions sufficient for the existence of two equilibriums are obtained. Third, the local stability of the two equilibriums is studied. Next, some numerical simulations are performed to verify the theoretical results. The mathematical and simulation results show that the values of some parameters, such as fractional order and medical resources, are critical for the stability of the equilibriums.
Aspen Plus® Modeling and Simulation of an Industrial Biomass Direct Liquefaction Process
Authors: Cecílio Duarte M. , Gonçalves J. Ricardo M. , Correia Maria Joana Neiva , Mateus Maria Margarida
Published: May 2023

The current energy and climate crisis calls for immediate action in replacing fossil fuels with those derived from renewable sources. The Energreen process performs the direct liquefaction of biomass to produce a liquid biofuel for the cement industry and an aqueous solution of added-value compounds for further processing. The present work details the development of an Aspen Plus® model to simulate this biomass liquefaction process. The proposed model describes the Energreen liquefaction process using simplified reaction kinetics and thermodynamic models. The model was validated using data from a real liquefaction pilot plant with a deviation of 6.4%. The simulation, conducted with several biomass samples of variable compositions, showed that the process is robust enough to deal with different compositions and, due to the substitution of the fossil fuels presently used in the cement plant, it will allow savings of up to USD 102,000 per year to be achieved. Several analyses of the sensitivity of the results to the process variables were performed and it was possible to identify the reactor temperature and the reaction activation energy as the most impactful parameters on the process output. Overall, the results allow us to conclude that the proposed model is a solid framework for the optimization of industrial liquefaction processes.
Anisotropic Bi-Layer Hydrogel Actuator with pH-Responsive Color-Changing and Photothermal-Responsive Shape-Changing Bi-Functional Synergy
Authors: Ma Chao , Peng Shuyi , Chen Lian , Cao Xingyu , Sun Ye , Chen Lin , Yang Lang , Ma Chunming , Liu Qijie , Liu Zhenzhong , Jiang Shaohua
Published: May 2023

Stimuli-responsive color-changing and shape-changing hydrogels are promising intelligent materials for visual detections and bio-inspired actuations, respectively. However, it is still an early stage to integrate the color-changing performance and shape-changing performance together to provide bi-functional synergistic biomimetic devices, which are difficult to design but will greatly expand further applications of intelligent hydrogels. Herein, we present an anisotropic bi-layer hydrogel by combining a pH-responsive rhodamine-B (RhB)-functionalized fluorescent hydrogel layer and a photothermal-responsive shape-changing melanin-added poly (N-isopropylacrylamide) (PNIPAM) hydrogel layer with fluorescent color-changing and shape-changing bi-functional synergy. This bi-layer hydrogel can obtain fast and complex actuations under irradiation with 808 nm near-infrared (NIR) light due to both the melanin-composited PNIPAM hydrogel with high efficiency of photothermal conversion and the anisotropic structure of this bi-hydrogel. Furthermore, the RhB-functionalized fluorescent hydrogel layer can provide rapid pH-responsive fluorescent color change, which can be integrated with NIR-responsive shape change to achieve bi-functional synergy. As a result, this bi-layer hydrogel can be designed using various biomimetic devices, which can show the actuating process in the dark for real-time tracking and even mimetic starfish to synchronously change both the color and shape. This work provides a new bi-layer hydrogel biomimetic actuator with color-changing and shape-changing bi-functional synergy, which will inspire new strategies for other intelligent composite materials and high-level biomimetic devices.
Rett-like Phenotypes in HNRNPH2-Related Neurodevelopmental Disorder
Authors: Gonzalez Joseph Nicho , Goldman Sylvie , Carter Melissa T. , Bain Jennifer M.
Published: May 2023

Rett Syndrome (RTT) is a neurodevelopmental disorder with a prevalence of 1:10,000 to 15,000 females worldwide. Classic Rett Syndrome presents in early childhood with a period of developmental regression, loss of purposeful hand skills along with hand stereotypies, gait abnormalities, and loss of acquired speech. Atypical RTT is diagnosed when a child shows some but not all the phenotypes of classic RTT, along with additional supporting criteria. Over 95% of classic RTT cases are attributed to pathogenic variants in Methyl-CpG Binding Protein 2 (MECP2), though additional genes have been implicated in other RTT cases, particularly those with the atypical RTT clinical picture. Other genetic etiologies have emerged with similar clinical characteristics to RTT Syndrome. Our team has characterized HNRNPH2-related neurodevelopmental disorder (HNRNPH2-RNDD) in 33 individuals associated with de novo pathogenic missense variants in the X-linked HNRNPH2 gene, characterized by developmental delay, intellectual disability, seizures, autistic-like features, and motor abnormalities. We sought to further characterize RTT clinical features in this group of individuals by using caregiver report. Twenty-six caregivers completed electronic surveys, with only 3 individuals having previously received an atypical RTT diagnosis, and no individuals with a typical RTT diagnosis. Caregivers reported a high number of behaviors and/or phenotypes consistent with RTT, including the major criteria of the syndrome, such as regression of developmental skills and abnormal gait. Based on the survey results, 12 individuals could meet the diagnostic clinical criteria for atypical RTT Syndrome. In summary, individuals with HNRNPH2-RNDD exhibit clinical characteristics that overlap with those of RTT, and therefore, HNRNPH2-RNDD, should be considered on the differential diagnosis list with this clinical picture.
Loess Strata Distribution Characteristics and Paleoclimate Spatial Pattern during the Last Interglacial in the Luohe River Basin
Authors: Guo Jiao , Shi Jiansheng , Chen Hongyun , Song Chao , Dong Qiuyao , Wang Wei
Published: May 2023

Paleoclimate studies of loess in China have focused mostly on the time series of a single borehole or profile. However, research on loess strata and regional paleoenvironmental patterns could facilitate a deeper understanding of loess as a paleoenvironmental indicator and provide new insights into interpreting loess in sedimentary records of the paleoclimate. In this study, we determined the spatial pattern and regional characteristics of the paleoclimate during the Last Interglacial period in the Luohe River Basin, Shaanxi Province, China. We selected four representative boreholes in the study area (ZK04, ZK18, ZK13, and ZK19) from different landforms and zones, distributed from the northwest to the southeast, as well as three classic profiles (JB, JD, and LC). From north to south, comparative analyses were conducted of the loess strata, magnetic susceptibility, and grain size, and we analyzed the distribution characteristics of loess and paleosols in different geomorphological regions. The results showed that both the thickness and the sedimentation rate of loess in this river basin decreased from north to south. There were few paleosol horizons in the northern Liangmao area, but numerous such horizons in the southern plateau, and the degree of paleosol development increased from north to south. The magnetic susceptibility increased, whereas the particle composition tended to become thinner from north to south. The climate fluctuations of the Last Glacial recorded by the loess and paleosols in different regions were inconsistent.
Identifying Key Themes of Care Coordination for Patients with Chronic Conditions in Singapore: A Scoping Review
Authors: Foo Chuan De , Yan Jia Yin , Chan Audrey Swee Ling , Yap Jason C H
Published: May 2023

A projected rise in patients with complex health needs and a rapidly ageing population will place an increased burden on the healthcare system. Care coordination can bridge potential gaps during care transitions and across the care continuum to facilitate care integration and the delivery of personalised care. Despite having a national strategic vision of improving care integration across different levels of care and community partners, there is no consolidation of evidence specifically on the salient dimensions of care coordination in the Singapore healthcare context. Hence, this scoping review aims to uncover the key themes that facilitate care coordination for patients with chronic conditions in Singapore to be managed in the community while illuminating under-researched areas in care coordination requiring further exploration. The databases searched were PubMed, CINAHL, Scopus, Embase, and Cochrane Library. Results from Google Scholar were also included. Two independent reviewers screened articles in a two-stage screening process based on the Cochrane scoping review guidelines. Recommendation for inclusion was indicated on a three-point scale and rating conflicts were resolved through discussion. Of the 5792 articles identified, 28 were included in the final review. Key cross-cutting themes such as having standards and guidelines for care programmes, forging stronger partnerships across providers, an interoperable information system across care interfaces, strong programme leadership, financial and technical resource availabilities and patient and provider-specific factors emerged. This review also recommends leveraging these themes to align with Singapore’s national healthcare vision to contain rising healthcare costs.
Relationship between the Social Development Index and Self-Reported Periodontal Conditions
Authors: Martínez-García Mireya , Rodríguez-Hernández Adriana-Patricia , Gutiérrez-Esparza Guadalupe O. , Castrejón-Pérez Roberto Carlos , Hernández-Lemus Enrique , Borges-Yáñez Socorro Aída
Published: May 2023

Inequalities in oral health are influenced by the social strata of the population. Few studies have focused on the multitude of factors related to social development as indicators of living conditions and periodontal health status. The aim of this study is to evaluate the association between self-reported periodontal conditions and the Social Development Index (SDI). A cross-sectional validated questionnaire was carried out among 1294 Mexican adults. Descriptive statistics and multivariate logistic regression models were used to identify the best predictors of self-reported periodontal conditions. Bone loss reporting was used as a proxy for the presence of periodontal disease. We found that higher global scores on the SDI and quality and available space in the home (QASH) increase the probability of having bone loss. Global SDI (OR = 7.27) and higher QASH (OR = 3.66) were indeed the leading societal factors related to periodontal disease. These results have pointed out how SDI and its indicators, in particular QASH, can be used to further explore inequities related to privileged access to dental care in the context of periodontal diseases.
“I Don’t Want to Go to Work”: A Mixed-Methods Analysis of Healthcare Worker Experiences from the Front- and Side-Lines of COVID-19
Authors: Heavner Smith F. , Stuenkel Mackenzie , Russ Sellers Rebecca , McCallus Rhiannon , Dean Kendall D. , Wilson Chloe , Shuffler Marissa , Britt Thomas W. , Stark Taylor Shannon , Benedum Molly , Munk Niki , Mayo Rachel , Cartmell Kathleen Buford , Griffin Sarah , Kennedy Ann Blair
Published: May 2023

During the COVID-19 pandemic, healthcare workers (HCW) were categorized as “essential” and “non-essential”, creating a division where some were “locked-in” a system with little ability to prepare for or control the oncoming crisis. Others were “locked-out” regardless of whether their skills might be useful. The purpose of this study was to systematically gather data over the course of the COVID-19 pandemic from HCW through an interprofessional lens to examine experiences of locked-out HCW. This convergent parallel mixed-methods study captured perspectives representing nearly two dozen professions through a survey, administered via social media, and video blogs. Analysis included logistic regression models of differences in outcome measures by professional category and Rapid Identification of Themes from Audio recordings (RITA) of video blogs. We collected 1299 baseline responses from 15 April 2020 to 16 March 2021. Of those responses, 12.1% reported no signs of burnout, while 21.9% reported four or more signs. Qualitative analysis identified four themes: (1) professional identity, (2) intrinsic stressors, (3) extrinsic factors, and (4) coping strategies. There are some differences in the experiences of locked-in and locked-out HCW. This did not always lead to differing reports of moral distress and burnout, and both groups struggled to cope with the realities of the pandemic.
Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release
Authors: Davies Rebecca , Allen Shannen , Mennan Claire , Platt Mark , Wright Karina , Kehoe Oksana
Published: May 2023

The immunomodulatory properties of MSCs can be recreated using their extracellular vesicles (EVs). Yet, the true capabilities of the MSC EVs cannot be distinguished from contaminating bovine EVs and protein derived from supplemental foetal bovine serum (FBS). FBS EV depletion protocols can minimise this, but vary in terms of depletion efficiency, which can negatively impact the cell phenotype. We explore the impact of FBS EV depletion strategies, including ultracentrifugation, ultrafiltration, and serum-free, on umbilical cord MSC characteristics. Whilst a greater depletion efficiency, seen in the ultrafiltration and serum-free strategies, did not impact the MSC markers or viability, the MSCs did become more fibroblastic, had slower proliferation, and showed inferior immunomodulatory capabilities. Upon MSC EV enrichment, more particles, with a greater particle/protein ratio, were isolated upon increasing the FBS depletion efficiency, except for serum-free, which showed a decreased particle number. Whilst all conditions showed the presence of EV-associated markers (CD9, CD63, and CD81), serum-free was shown to represent a higher proportion of these markers when normalised by total protein. Thus, we caution MSC EV researchers on the use of highly efficient EV depletion protocols, showing that it can impact the MSC phenotype, including their immunomodulatory properties, and stress the importance of testing in consideration to downstream objectives.
Transcription Factors-Regulated Leaf Senescence: Current Knowledge, Challenges and Approaches
Authors: Cao Jie , Liu Hairong , Tan Shuya , Li Zhonghai
Published: May 2023

Leaf senescence is a complex biological process regulated at multiple levels, including chromatin remodeling, transcription, post-transcription, translation, and post-translational modifications. Transcription factors (TFs) are crucial regulators of leaf senescence, with NAC and WRKY families being the most studied. This review summarizes the progress made in understanding the regulatory roles of these families in leaf senescence in Arabidopsis and various crops such as wheat, maize, sorghum, and rice. Additionally, we review the regulatory functions of other families, such as ERF, bHLH, bZIP, and MYB. Unraveling the mechanisms of leaf senescence regulated by TFs has the potential to improve crop yield and quality through molecular breeding. While significant progress has been made in leaf senescence research in recent years, our understanding of the molecular regulatory mechanisms underlying this process is still incomplete. This review also discusses the challenges and opportunities in leaf senescence research, with suggestions for possible strategies to address them.
The Transcription Factor Nrf2 Mediates the Effects of Antrodia camphorata Extract on Neuropathological Changes in a Mouse Model of Parkinson’s Disease
Authors: Lanza Marika , Cucinotta Laura , Casili Giovanna , Filippone Alessia , Basilotta Rossella , Capra Anna Paola , Campolo Michela , Paterniti Irene , Cuzzocrea Salvatore , Esposito Emanuela
Published: May 2023

Parkinson’s disease (PD) is a disorder that is characterized by progressive and selective neuronal injury and cell death. Recent studies have provided accumulating evidence for a significant role of the immune system and neuroinflammation in PD pathogenesis. On this basis, many scientific articles have highlighted the anti-inflammatory and neuroprotective properties of Antrodia camphorata (AC), an edible fungus containing various bioactive compounds. This study aimed to evaluate the inhibitory effect of AC administration on neuroinflammation and oxidative stress in a murine model of MPTP-induced dopaminergic degeneration. AC (10, 30, 100 mg/kg) was administered daily by oral gavage starting 24 h after the first administration of MPTP, and mice were sacrificed 7 days after MPTP induction. In this study, treatment with AC significantly reduced the alteration of PD hallmarks, increasing tyrosine hydroxylase expression and reducing the number of alpha-synuclein-positive neurons. In addition, AC treatment restored the myelination process of neurons associated with PD and attenuated the neuroinflammatory state. Furthermore, our study demonstrated that AC was able to reduce the oxidative stress induced by MPTP injection. In conclusion, this study highlighted that AC could be a potential therapeutic agent for the treatment of neurodegenerative disorders such as PD.
Antibacterial Activity and Mechanisms of TroHepc2-22, a Derived Peptide of Hepcidin2 from Golden Pompano (Trachinotus ovatus)
Authors: Zhang Zhengshi , Zhou Yongcan , Zhang Han , Du Xiangyu , Cao Zhenjie , Wu Ying , Liu Chunsheng , Sun Yun
Published: May 2023

Hepcidin, a cysteine-rich antimicrobial peptide, has a highly conserved gene structure in teleosts, and it plays an essential role in host immune response against various pathogenic bacteria. Nonetheless, few studies on the antibacterial mechanism of hepcidin in golden pompano (Trachinotus ovatus) have been reported. In this study, we synthesized a derived peptide, TroHepc2-22, from the mature peptide of T. ovatus hepcidin2. Our results showed that TroHepc2-22 has superior antibacterial abilities against both Gram-negative (Vibrio harveyi and Edwardsiella piscicida) and Gram-positive (Staphylococcus aureus and Streptococcus agalactiae) bacteria. Based on the results of a bacterial membrane depolarization assay and propidium iodide (PI) staining assay in vitro, TroHepc2-22 displayed antimicrobial activity by inducing the bacterial membrane depolarization and changing the bacterial membrane permeability. Scanning electron microscopy (SEM) visualization illustrated that TroHepc2-22 brought about membrane rupturing and the leakage of the cytoplasm for the bacteria. In addition, TroHepc2-22 was verified to have hydrolytic activity on bacterial genomic DNA in view of the results of the gel retardation assay. In terms of the in vivo assay, the bacterial loads of V. harveyi in the tested immune tissues (liver, spleen, and head kidney) were significantly reduced in T. ovatus, revealing that TroHepc2-22 significantly enhanced the resistance against V. harveyi infection. Furthermore, the expressions of immune-related genes, including tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin 1-β (IL-1β), IL-6, Toll-like receptor 1 (TLR1), and myeloid differentiation factor 88 (MyD88) were significantly increased, indicating that TroHepc2-22 might regulate inflammatory cytokines and activate immune-related signaling pathways. To summarize, TroHepc2-22 possesses appreciable antimicrobial activity and plays a vital role in resisting bacterial infection. The observation of our present study unveils the excellent application prospect of hepcidin as a substitute for antibiotics to resist pathogenic microorganisms in teleosts.
The Regulation of the Growth and Pathogenicity of Valsa mali by the Carbon Metabolism Repressor CreA
Authors: Jin Jiyang , Diao Yufei , Xiong Xiong , Yu Chengming , Tian Yehan , Li Chuanrong , Liu Huixiang
Published: May 2023

Carbon catabolite repression (CCR) is a very important mechanism for efficient use of carbon sources in the environment and is necessary for the regulation of fungal growth, development, and pathogenesis. Although there have been extensive studies conducted regarding this mechanism in fungi, little is yet known about the effects of CreA genes on Valsa mali. However, based on the results obtained in this study for the identification of the VmCreA gene in V. mali, it was determined that the gene was expressed at all stages of fungal growth, with self-repression observed at the transcriptional level. Furthermore, the functional analysis results of the gene deletion mutants (ΔVmCreA) and complements (CTΔVmCreA) showed that the VmCreA gene played an important role in the growth, development, pathogenicity, and carbon source utilization of V. mali.
The Methylation Analysis of the Glucose-Dependent Insulinotropic Polypeptide Receptor (GIPR) Locus in GH-Secreting Pituitary Adenomas
Authors: Dalle Nogare Mattia , D’Annunzio Sarah , Vazza Giovanni , Regazzo Daniela , Picello Luna , Denaro Luca , Voltan Giacomo , Scaroni Carla , Ceccato Filippo , Occhi Gianluca
Published: May 2023

The glucose-dependent insulinotropic polypeptide receptor (GIPR) is aberrantly expressed in about one-third of GH-secreting pituitary adenomas (GH-PAs) and has been associated with a paradoxical increase of GH after a glucose load. The reason for such an overexpression has not yet been clarified. In this work, we aimed to evaluate whether locus-specific changes in DNA methylation patterns could contribute to this phenomenon. By cloning bisulfite-sequencing PCR, we compared the methylation pattern of the GIPR locus in GIPR-positive (GIPR+) and GIPR-negative (GIPR−) GH-PAs. Then, to assess the correlation between Gipr expression and locus methylation, we induced global DNA methylation changes by treating the lactosomatotroph GH3 cells with 5-aza-2′-deoxycytidine. Differences in methylation levels were observed between GIPR+ and GIPR− GH-PAs, both within the promoter (31.9% vs. 68.2%, p < 0.05) and at two gene body regions (GB_1 20.7% vs. 9.1%; GB_2 51.2% vs. 65.8%, p < 0.05). GH3 cells treated with 5-aza-2′-deoxycytidine showed a ~75% reduction in Gipr steady-state level, possibly associated with the observed decrease in CpGs methylation. These results indicate that epigenetic regulation affects GIPR expression in GH-PAs, even though this possibly represents only a part of a much more complex regulatory mechanism.
Nutrition and Aging
Authors: Mohajeri M. Hasan
Published: May 2023

This Special Issue focuses on the importance of nutritional interventions for the delay of age-related conditions [...]
Polymeric Wet-Strength Agents in the Paper Industry: An Overview of Mechanisms and Current Challenges
Authors: Francolini Iolanda , Galantini Luciano , Rea Fernando , Di Cosimo Cristiano , Di Cosimo Pierpaolo
Published: May 2023

Polymeric wet-strength agents are important additives used in the paper industry to improve the mechanical properties of paper products, especially when they come into contact with water. These agents play a crucial role in enhancing the durability, strength, and dimensional stability of paper products. The aim of this review is to provide an overview of the different types of wet-strength agents available and their mechanisms of action. We will also discuss the challenges associated with the use of wet-strength agents and the recent advances in the development of more sustainable and environmentally friendly agents. As the demand for more sustainable and durable paper products continues to grow, the use of wet-strength agents is expected to increase in the coming years.
Supercritical Impregnation of Mesoglycan and Lactoferrin on Polyurethane Electrospun Fibers for Wound Healing Applications
Authors: Mottola Stefania , Viscusi Gianluca , Iannone Giovanna , Belvedere Raffaella , Petrella Antonello , De Marco Iolanda , Gorrasi Giuliana
Published: May 2023

Fibrous membranes of thermoplastic polyurethane (TPU) were fabricated through a uni-axial electrospinning process. Fibers were then separately charged with two pharmacological agents, mesoglycan (MSG) and lactoferrin (LF), by supercritical CO2 impregnation. Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analysis proved the formation of a micrometric structure with a homogeneous distribution of mesoglycan and lactoferrin. Besides, the degree of retention is calculated in four liquid media with different pHs. At the same time, angle contact analysis proved the formation of a hydrophobic membrane loaded with MSG and a hydrophilic LF-loaded one. The impregnation kinetics demonstrated a maximum loaded amount equal to 0.18 ± 0.20% and 0.07 ± 0.05% for MSG and LT, respectively. In vitro tests were performed using a Franz diffusion cell to simulate the contact with the human skin. The release of MSG reaches a plateau after about 28 h while LF release leveled off after 15 h. The in vitro compatibility of electrospun membranes has been evaluated on HaCaT and BJ cell lines, as human keratinocytes and fibroblasts, respectively. The reported data proved the potential application of fabricated membranes for wound healing.
Simulation of Hemorrhage Pathogenesis in Mice through Dual Stimulation with Dengue Envelope Protein Domain III-Coated Nanoparticles and Antiplatelet Antibody
Authors: Lien Te-Sheng , Sun Der-Shan , Wu Wen-Sheng , Chang Hsin-Hou
Published: May 2023

Dengue hemorrhagic fever (DHF) is a severe form of dengue virus (DENV) infection that can lead to abnormal immune responses, endothelial vascular dysfunction, and hemorrhage pathogenesis. The virion-associated envelope protein domain III (EIII) is thought to play a role in the virulence of DENV by damaging endothelial cells. However, it is unclear whether EIII-coated nanoparticles simulating DENV virus particles could cause a more severe pathogenesis than soluble EIII alone. This study aimed to investigate whether EIII-coated silica nanoparticles (EIII-SNPs) could elicit greater cytotoxicity in endothelial cells and hemorrhage pathogenesis in mice compared to EIII or silica nanoparticles alone. The main methods included in vitro assays to assess cytotoxicity and in vivo experiments to examine hemorrhage pathogenesis in mice. EIII-SNPs induced greater endothelial cytotoxicity in vitro than EIII or silica nanoparticles alone. Two-hit combined treatment with EIII-SNPs and antiplatelet antibodies to simulate DHF hemorrhage pathogenesis during secondary DENV infections resulted in higher endothelial cytotoxicity than either treatment alone. In mouse experiments, two-hit combined treatment with EIII-SNPs and antiplatelet antibodies resulted in more severe hemorrhage pathogenesis compared to single treatments of EIII, EIII-SNPs, or antiplatelet antibodies alone. These findings suggest that EIII-coated nanoparticles are more cytotoxic than soluble EIII and could be used to develop a tentative dengue two-hit hemorrhage pathogenesis model in mice. Additionally, our results indicated that EIII-containing DENV particles could potentially exacerbate hemorrhage pathogenesis in DHF patients who have antiplatelet antibodies, highlighting the need for further research on the potential role of EIII in DHF pathogenesis.
Transcriptome Profiling of Prostate Cancer, Considering Risk Groups and the TMPRSS2-ERG Molecular Subtype
Authors: Kobelyatskaya Anastasiya A. , Pudova Elena A. , Katunina Irina V. , Snezhkina Anastasiya V. , Fedorova Maria S. , Pavlov Vladislav S. , Kotelnikova Anastasiya O. , Nyushko Kirill M. , Alekseev Boris Y. , Krasnov George S. , Kudryavtseva Anna V.
Published: May 2023

Molecular heterogeneity in prostate cancer (PCa) is one of the key reasons underlying the differing likelihoods of recurrence after surgical treatment in individual patients of the same clinical category. In this study, we performed RNA-Seq profiling of 58 localized PCa and 43 locally advanced PCa tissue samples obtained as a result of radical prostatectomy on a cohort of Russian patients. Based on bioinformatics analysis, we examined features of the transcriptome profiles within the high-risk group, including within the most commonly represented molecular subtype, TMPRSS2-ERG. The most significantly affected biological processes in the samples were also identified, so that they may be further studied in the search for new potential therapeutic targets for the categories of PCa under consideration. The highest predictive potential was found with the EEF1A1P5, RPLP0P6, ZNF483, CIBAR1, HECTD2, OGN, and CLIC4 genes. We also reviewed the main transcriptome changes in the groups at intermediate risk of PCa—Gleason Score 7 (groups 2 and 3 according to the ISUP classification)—on the basis of which the LPL, MYC, and TWIST1 genes were identified as promising additional prognostic markers, the statistical significance of which was confirmed using qPCR validation.
A Combination of the Immunotherapeutic Drug Anti-Programmed Death 1 with Lenalidomide Enhances Specific T Cell Immune Responses against Acute Myeloid Leukemia Cells
Authors: Guinn Barbara-ann , Schuler Patrick J. , Schrezenmeier Hubert , Hofmann Susanne , Weiss Johanna , Bulach Christiane , Götz Marlies , Greiner Jochen
Published: May 2023

Immune checkpoint inhibitors can block inhibitory molecules on the surface of T cells, switching them from an exhausted to an active state. One of these inhibitory immune checkpoints, programmed cell death protein 1 (PD-1) is expressed on T cell subpopulations in acute myeloid leukemia (AML). PD-1 expression has been shown to increase with AML progression following allo-haematopoeitic stem cell transplantation, and therapy with hypomethylating agents. We have previously shown that anti-PD-1 can enhance the response of leukemia-associated antigen (LAA)-specific T cells against AML cells as well as leukemic stem and leukemic progenitor cells (LSC/LPCs) ex vivo. In concurrence, blocking of PD-1 with antibodies such as nivolumab has been shown to enhance response rates post-chemotherapy and stem cell transplant. The immune modulating drug lenalidomide has been shown to promote anti-tumour immunity including anti-inflammatory, anti-proliferative, pro-apoptotic and anti-angiogenicity. The effects of lenalidomide are distinct from chemotherapy, hypomethylating agents or kinase inhibitors, making lenalidomide an attractive agent for use in AML and in combination with existing active agents. To determine whether anti-PD-1 (nivolumab) and lenalidomide alone or in combination could enhance LAA-specific T cell immune responses, we used colony-forming immune and ELISpot assays. Combinations of immunotherapeutic approaches are believed to increase antigen-specific immune responses against leukemic cells including LPC/LSCs. In this study we used a combination of LAA-peptides with the immune checkpoint inhibitor anti-PD-1 and lenalidomide to enhance the killing of LSC/LPCs ex vivo. Our data offer a novel insight into how we could improve AML patient responses to treatment in future clinical studies.
Research Progress in Improving Photosynthetic Efficiency
Authors: Li Ruiqi , He Ying , Chen Junyu , Zheng Shaoyan , Zhuang Chuxiong
Published: May 2023

Photosynthesis is the largest mass- and energy-conversion process on Earth, and it is the material basis for almost all biological activities. The efficiency of converting absorbed light energy into energy substances during photosynthesis is very low compared to theoretical values. Based on the importance of photosynthesis, this article summarizes the latest progress in improving photosynthesis efficiency from various perspectives. The main way to improve photosynthetic efficiency is to optimize the light reactions, including increasing light absorption and conversion, accelerating the recovery of non-photochemical quenching, modifying enzymes in the Calvin cycle, introducing carbon concentration mechanisms into C3 plants, rebuilding the photorespiration pathway, de novo synthesis, and changing stomatal conductance. These developments indicate that there is significant room for improvement in photosynthesis, providing support for improving crop yields and mitigating changes in climate conditions.
Immunohistochemistry Reveals TRPC Channels in the Human Hearing Organ—A Novel CT-Guided Approach to the Cochlea
Authors: Englisch Colya N. , Steinhäuser Jakob , Wemmert Silke , Jung Martin , Gawlitza Joshua , Wenzel Gentiana , Schick Bernhard , Tschernig Thomas
Published: May 2023

TRPC channels are critical players in cochlear hair cells and sensory neurons, as demonstrated in animal experiments. However, evidence for TRPC expression in the human cochlea is still lacking. This reflects the logistic and practical difficulties in obtaining human cochleae. The purpose of this study was to detect TRPC6, TRPC5 and TRPC3 in the human cochlea. Temporal bone pairs were excised from ten body donors, and the inner ear was first assessed based on computed tomography scans. Decalcification was then performed using 20% EDTA solutions. Immunohistochemistry with knockout-tested antibodies followed. The organ of Corti, the stria vascularis, the spiral lamina, spiral ganglion neurons and cochlear nerves were specifically stained. This unique report of TRPC channels in the human cochlea supports the hypothesis of the potentially critical role of TRPC channels in human cochlear health and disease which has been suggested in previous rodent experiments.
Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica
Authors: Liu Guodong , Rui Lin , Yang Yuying , Liu Ranxin , Li Hongliang , Ye Fan , You Chunxiang , Zhang Shuai
Published: May 2023

Nitrate is one of the main sources of nitrogen for plant growth. Nitrate transporters (NRTs) participate in nitrate uptake and transport, and they are involved in abiotic stress tolerance. Previous studies have shown that NRT1.1 has a dual role in nitrate uptake and utilization; however, little is known about the function of MdNRT1.1 in regulating apple growth and nitrate uptake. In this study, apple MdNRT1.1, a homolog of Arabidopsis NRT1.1, was cloned and functionally identified. Nitrate treatment induced an increased transcript level of MdNRT1.1, and overexpression of MdNRT1.1 promoted root development and nitrogen utilization. Ectopic expression of MdNRT1.1 in Arabidopsis repressed tolerance to drought, salt, and ABA stresses. Overall, this study identified a nitrate transporter, MdNRT1.1, in apples and revealed how MdNRT1.1 regulates nitrate utilization and abiotic stress tolerance.