bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptomics integrated with widely targeted metabolomics reveals the cold resistance mechanism in Hevea brasiliensis
Authors: Mao Changli , Li Ling , Yang Tian , Gui Mingchun , Li Xiaoqin , Zhang Fengliang , Zhao Qi , Wu Yu
Published: January 2023

The rubber tree is the primary source of natural rubber and is mainly cultivated in Southeast Asian countries. Low temperature is the major abiotic stress affecting the yield of the rubber tree. Therefore, uncovering the cold resistance mechanism in the rubber tree is necessary. The present study used RNA-sequencing technology and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to analyze the transcriptomic and metabolomic changes in two rubber tree clones with different cold resistance capacities (temperature-sensitive Reyan 8-79 and cold-resistant Yunyan 77-4) at 0 h, 2 h, 6 h, and 20 h of exposure to 4°C. Independent analysis of the transcriptome and metabolitome showed that under prolonged low-temperature treatment, Yunyan 77-4 expressed more genes involved in regulating enzyme activity, changing cell permeability, and synthesizing significant metabolites, such as flavonoids and amino acids, than Reyan 8-79. The KEGG annotation and enrichment analysis identified arginine metabolism and biosynthesis of flavonoids as the major pathway associated with cold resistance. Integrated transcriptome and metabolome analysis showed that the increase in the expression of genes modulated flavonoid biosynthesis, arginine biosynthesis, and anthocyanins biosynthesis, resulting in higher levels of metabolites, such as naringenin chalcone, apigenin, dihydroquercetin, cyanidin 3-glucoside, L-arginosuccinate, N-acetyl-ornithine, ornithine, and N-acetyl-glutamate, in Yunyan 77-4 than in Reyan 8-79 after prolonged low-temperature treatment. Phylogenetic analysis identified the genes, such as CHS (gene356) and F3H (gene33147) of flavonoid biosynthesis and NAGS (gene16028, gene33765), ArgC (gene2487), and ASS (gene6161) of arginine biosynthesis were the key genes involved in the cold resistant of rubber tree. Thus, the present study provides novel insights into how rubber clones resist cold and is a valuable reference for cold-resistance breeding.
Gene expression profiling in Rosa roxburghii fruit and overexpressing RrGGP2 in tobacco and tomato indicates the key control point of AsA biosynthesis
Authors: Yan Yali , Liu Yiyi , Lu Min , Lu Chen , Ludlow Richard A. , Yang Man , Huang Wei , Liu Zeyang , An HuaMing
Published: January 2023

Rosa roxburghii Tratt. is an important commercial horticultural crop endemic to China, which is recognized for its extremely high content of L-ascorbic acid (AsA). To understand the mechanisms underlying AsA overproduction in fruit of R. roxburghii, content levels, accumulation rate, and the expression of genes putatively in the biosynthesis of AsA during fruit development have been characterized. The content of AsA increased with fruit weight during development, and AsA accumulation rate was found to be highest between 60 and 90 days after anthesis (DAA), with approximately 60% of the total amount being accumulated during this period. In vitro incubating analysis of 70DAA fruit flesh tissues confirmed that AsA was synthesized mainly via the L-galactose pathway although L-Gulono-1, 4-lactone was also an effective precursor elevating AsA biosynthesis. Furthermore, in transcript level, AsA content was significantly associated with GDP-L-galactose phosphorylase (RrGGP2) gene expression. Virus-induced RrGGP2 silencing reduced the AsA content in R. roxburghii fruit by 28.9%. Overexpressing RrGGP2 increased AsA content by 8-12-fold in tobacco leaves and 2.33-3.11-fold in tomato fruit, respectively, and it showed enhanced resistance to oxidative stress caused by paraquat in transformed tobacco. These results further justified the importance of RrGGP2 as a major control step to AsA biosynthesis in R. roxburghii fruit.
The role of corepressor HOS15-mediated epigenetic regulation of flowering
Authors: Huang Li-Jun , Wang Yukun , Lin Zeng , Jiang Dong , Luo Yong , Li Ning
Published: January 2023

Regulation of gene expression underpins gene function and is essential for regulation of physiological roles. Epigenetic modifications regulate gene transcription by physically facilitating relaxation or condensation of target loci in chromatin. Transcriptional corepressors are involved in chromatin remodeling and regulate gene expression by establishing repressive complexes. Genetic and biochemical studies reveal that a member of the Groucho/Thymidine uptake 1 (Gro/Tup1) corepressor family, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15), is recruited via the evening complex (EC) to the GIGANTEA (GI) promoter to repress gene expression, and modulating flowering time. Therefore, HOS15 connects photoperiodic pathway and epigenetic mechanism to control flowering time in plants. In addition, growing body of evidence support a diverse roles of the epigenetic regulator HOS15 in fine-tuning plant development and growth by integrating intrinsic genetic components and various environmental signals.
The use of theory of planned behavior to systemically study the integrative-qualitative intentional behavior in Romanian preschool education with network analysis
Authors: Rad Dana , Redeș Adela , Roman Alina , Egerău Anca , Lile Raul , Demeter Edgar , Dughi Tiberiu , Ignat Sonia , Balaș Evelina , Maier Roxana , Kiss Csaba , Mărineanu Vasile , Bocoș Mușata , Bâtcă-Dumitru Graziella Corina , Cuc Lavinia Denisia , Vancu Gabriela , Rad Gavril , Chiș Roxana
Published: January 2023

Predicting preschool teachers’ intention to adopt qualitative and inclusive early childhood intentional behaviors represents an important research field. The objective of this research is first to develop and validate a scale to assess the integrative-qualitative intentional behavior (IQIB-ECEC) of preschool teachers in order to achieve SDG4.2’s objective of ensuring that all children have access to high-quality pre-primary education and then to systemically analyze the relationship between variables with Network Analysis. The theory of planned behavior (TPB) describes key individual beliefs (attitudes, subjective norms, and perceived behavior control) that affect people’s intentions to engage in a certain conduct and has previously been used with success in evaluating people’s intentions to adopt a certain behavior. This research represents one of the first Romanian attempts to use the theory of planned behavior to study the Integrative-Qualitative Intentional Behavior in Romanian Preschool Education and systemically analyze results with Network Analysis approach. This study used a randomized 300 Romanian preschool teachers enrolled in a National Training Program entitled Qualitative and Inclusive Early Childhood Education managed by the Romanian Educational Ministry. Data were collected via an online questionnaire. The scale validation followed a confirmatory factor analysis (CFA). The fitting of the IQIB-ECEC 19-item scale showed that all coefficients CFI (0.942), TLI (0.920), SRMR (0.0452), and RMSEA (0.0451) bring strong evidence in the favor of the statistical validity of the scale. The final IQIB-ECEC 19 items and 8 factors scale obtained a Cronbach’s alpha of 0.77. The systemic Network Analysis approach was used in interpreting data. The centrality of the network model was further investigated and the clustering coefficients index were calculated. According to the results, perceived power/control beliefs and behavioral intention were detected as the most important dimensions, whereas behavioral beliefs were less important. These findings were discussed in terms of their theoretical and practical significance.
The relationship between physical exercise and subjective well-being among Chinese junior high school students: A chain mediating model
Authors: Yao Shu-Jun , Ma Qi-Shuai , Liu Chao , Cao Da-Wei , Lyu Teng , Guo Ke-Lei
Published: January 2023

Objective

This study aims to help understand the mechanism behind the relationship between physical exercise and the subjective well-being among Chinese junior high school students, and it is of great significance for the intervention measures to improve the subjective well-being of junior high school students.

Methods

Using stratified cluster sampling method, 1,510 junior high school students (727 males and 783 females) were measured by physical exercise rating scale, perceived social support scale, physical exercise self-efficacy scale, and subjective well-being scale. For data analysis, Pearson’s correlation analysis, structural equation model test, and bias-corrected percentile Bootstrap method were carried out in turn.

Results

Common method biases can be accepted in this study. (1) There is a positive correlation between physical exercise and subjective well-being, and physical exercise has a significant predictive effect on subjective well-being(β = 0.367 t = 9.415 p < 0.01); (2) Perceived social support partially mediated the relationship between physical exercise and subjective well-being (β = 0.08, t = 3.083, p < 0.01), and its effect ratio is 78.047%; (3) Physical exercise self-efficacy plays a partial mediating role between physical exercise and subjective well-being(β = 0.181, t = 5.132, p < 0.01), accounting for 50.632%; (4) The chain mediating effect of perceived social support and physical exercise self-efficacy was significant (the mediating effect value was 0.028), and the effect amount was 7.629%.

Conclusion

(1) Physical exercise can significantly positively predict the subjective well-being of junior high school students; (2) Physical exercise can also indirectly affect the subjective well-being of junior high school students through the mediating effect of perceived social support and physical exercise self-efficacy. The intermediary effect includes three paths, namely, the individual intermediary effect of perceived social support and physical exercise self-efficacy and the chain intermediary effect of perceived social support physical exercise self-efficacy.
An in-depth analysis of the representation of speech acts and language functions in Libyan public high school English textbooks
Authors: Alhadi Ali Ahmed Fatma , Mohammadzadeh Behbood , Mazlum Farhad
Published: January 2023

Researchers have taken an interest to study the language of English materials that learners use in different contexts. One line of inquiry is to examine the pragmatic competence potential of such materials. This study aimed at examining the use of speech acts and language functions in the 21st Century English for Libya Secondary 1, 2, and 3 course books and workbooks. To achieve this aim, a frequency-based analysis of language functions and speech acts in the conversation sections in the five course books and five workbooks was followed. A data analysis revealed that the distribution of speech acts and language functions is highly skewed; in each analysis domain, a given category accounted for almost half of the data while other categories accounted for little or even none. Findings are used to argue that Libyan students need a more balanced and language-in-use informed distribution of English functions and speech acts to learn the pragmatic features of English; otherwise, they are less likely to become communicatively competent in speech acts and functions that have been not been treated adequately. The findings also highlight that the unequal frequency and distribution of these pragmatic variables are in line with previous studies and provide implications for English as a Foreign Language (EFL) curriculum, material designers, and language practitioners.
Effects of immersion in a simulated natural environment on stress reduction and emotional arousal: A systematic review and meta-analysis
Authors: Li Hongyi , Ding Yujun , Zhao Bing , Xu Yuhang , Wei Wei
Published: January 2023

Background

Although the mental health benefits of exposure to simulated natural environments are well established by researchers from environmental psychology, landscape architecture, and public health, it is unclear whether and to what extent technological immersion affects these benefits.

Methods

Systematical literature searches were conducted in May 2022 from six databases. The risk of bias was evaluated using the Cochrane’s Risk of Bias tool 2.0 and the Risk of Bias in Non-randomized Studies of Interventions tool. We performed a random-effects meta-regression to investigate the heterogeneity. The immersion levels of included studies were classified by projection devices and motion capture, and then subgroup analysis was conducted.

Results

Twenty-six publications were included. Exposure to simulated nature was confirmed to be associated with increased positive affect 0.40 [95% confidence interval (CI): 0.22, 0.58], vigor 0.58 (95% CI: 0.30, 0.86), calmness 0.54 (95% CI: 0.17, 0.92) and decreased perceived stress −0.38 (95% CI: −0.71, −0.06), total mood disturbance −0.87 (95% CI: −1.17, −0.57), tension −0.70 (95% CI: −0.99, −0.41), fatigue −0.60 (95% CI: −0.91, −0.28), anxiety −0.72 (95% CI: −1.43, −0.02), depression −0.33 (95% CI: −0.52, −0.14), confusion −0.79 (95% CI: −1.19, −0.40), and anger −0.54 (95% CI: −0.76, −0.31). Gender, health status, study design, mean age, and single exposure duration were not significant when entered in a meta-regression. For positive affect, medium immersion was observed to produce a larger effect than low and high immersion. All included studies had a moderate to high risk of bias.

Conclusion

Audio-visual exposure to simulated nature contributes to stress relief and emotional arousal. The immersion level explains the heterogeneity of positive affect triggered by simulated nature. Focusing on the technical features will open up new possibilities for combining actual and simulated nature’s mental health benefits.
Soundwalk path affecting soundscape assessment in urban parks
Authors: Sun Chutian , Meng Qi , Yang Da , Wu Yue
Published: January 2023

Introduction

Previous soundscape studies focused on sound perception and acoustic comfort in sampled sites and ignored the characteristics of the experiential process along the paths. Therefore, the effects of soundwalk paths on soundscape assessment should be explored.

Methods

In this study, a typical urban park was selected as a case study. The differences, characteristics, and effects of soundwalk paths on the soundscapes were explored, based on a soundwalk survey and laboratory study.

Results

The results are as follows: first, in the soundwalk, different soundwalk paths in the urban park had significant effects on the perceived extent of individual sound and soundscape assessment. Second, the soundscape assessment was consistent with the peak-end rule. In the laboratory experiments, the peak assessment of soundscape appeared at the end location of the soundwalk paths, it was critical to the overall soundscape assessment. Furthermore, in the soundwalk, the overall perceived extent of individual sound was strongly affected by the perceived extent of dominant sounds at the end location of the soundwalk paths. Third, in the soundwalk, the sound loudness contrast path (noise-quiet/quiet-noise) and sound source contrast path (natural-artificial sound/artificial-natural sound) were compared. In the noise-quiet path, the assessment of acoustic comfort was higher than that in the quiet-noise path, and the assessment of subjective loudness was lower than that in the quiet-noise path (p < 0.01). The subjective loudness of the artificial-natural sound path was lower than that in the natural-artificial sound path (p < 0.05).

Discussion

Thus, the design of the soundwalk paths was expected to enhance visitors’ soundscape assessment of urban parks.
Sex- and genotype-dependent nicotine plus cue-primed reinstatement is enhanced in adolescent Sprague Dawley rats containing the human CHRNA6 3′-UTR polymorphism (rs2304297)
Authors: Carreño Diana , Lotfipour Shahrdad
Published: January 2023

Rationale

Large-scale human candidate gene studies have indicated that a genetic variant (rs2304297) in the alpha(α)6 nicotinic acetylcholine receptor (nAChR) subunit, encoded by the CHRNA6 gene, may play a key role in adolescent nicotine addictive behavior. We hypothesized that the polymorphism selectively enhances nicotine + cue-primed reinstatement, but not nicotine- or cue-reinstatement in α6GG (risk) vs. α6CC (non-risk) allele carriers, without having baseline effects on natural rewards.

Methods

Using CRISPR-Cas9 genomic engineering, we developed a humanized rat line with the human gene variant of the CHRNA6 3′-UTRC123G polymorphism in Sprague-Dawley rats. Genetically modified adolescent male and female rats were food trained under a fixed-ratio (FR)1 schedule of reinforcement and progressively increased to FR5. Animals were implanted with catheters and began nicotine self-administration (15 μg/kg/infusion) at FR5. Upon reaching stable responding, reinforced behavior was extinguished by removal of drug and cues. Reinstatement testing began for cue only, nicotine only, and nicotine + cue in a Latin Square Design. Animals were returned to extinction conditions for 2 days minimum between testing.

Results

For natural food rewards, nicotine self-administration, progressive ratio, and extinction, adolescent male and female (α6GG and α6CC) rats exhibited equivalent behaviors. Male α6GG rats show enhanced nicotine + cue-primed reinstatement when compared with male α6CC rats. This genotype effect on reinstatement was not seen in female rats.

Conclusion

Our findings support the in vivo functional role of the human CHRNA6 3′-UTR SNP genetic variant in sex-dependently enhancing nicotine seeking behavior in adolescent rats. Overall, the findings support clinical and preclinical data highlighting a role of α6 nAChRs mediating sex heterogeneity in substance use and related phenotypes.
Clinical supervision approach predicts evidence-based trauma treatment delivery in children’s mental health
Authors: Meza Rosemary D. , AlRasheed Rashed , Pullmann Michael D. , Dorsey Shannon
Published: January 2023

Objective

Observational studies of practices used in clinical supervision-as-usual can be leveraged to advance the limited research on workplace-based supervision as an evidence-based treatment (EBT) implementation strategy. This exploratory observational study examined the presence of supervision approaches (comprised of supervision techniques) and whether these predicted clinicians’ EBT technique delivery.

Methods

Participants included 28 supervisors, 70 clinician supervisees, and 60 youth clients and guardians from 17 public mental health organizations. Data included audio recorded supervision-as-usual sessions over 1 year, audio recorded Trauma-focused Cognitive Behavioral Therapy (TF-CBT) treatment sessions with youth for 6 months, and youth-reported post-traumatic stress severity scores. Audio recordings of 438 supervision sessions were coded for session duration and the presence of 13 supervision techniques and intensity of their coverage. Audio recordings of 465 treatment sessions were coded for presence and intensity of coverage of TF-CBT practice elements. Agglomerative hierarchical cluster analysis examined the presence of clusters of supervision technique use, termed supervision approaches. Generalized estimating equations estimated the relation between supervision approaches and delivery of TF-CBT elements.

Results

Two supervision approaches were identified– Supportive–Directive and Supportive– that discriminated between use of five supervision techniques. Clinicians who received a higher proportion of supportive–directive supervision sessions had greater odds of delivering the trauma narrative with a client.

Conclusion

Findings suggest that patterns of supervision techniques can be identified and may shape EBT delivery. Supervision approaches show some evidence of being tailored to individual clinicians. Implications for the development of supervision implementation strategies and future directions are discussed.
Case report: Ketogenic diet acutely improves cognitive function in patient with Down syndrome and Alzheimer’s disease
Authors: Bosworth Annette , Loh Vyvyane , Stranahan Blackjack N. , Palmer Christopher M.
Published: January 2023

Ketogenic diets have a century-long history as a therapeutic tool to treat intractable epilepsy. Recently, a renewed interest in neuroketotherapeutics has arisen, with ketogenic diets being explored for the treatment of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease, as well as mental health conditions. Herein, we present a case report of a 47-year-old woman with Down syndrome diagnosed with Alzheimer’s disease and absence seizures with accelerated cognitive decline over 6 years. A ketogenic diet restored her cognitive function over 6 weeks, with an increase in Activities of Daily Living Scale score from 34 to 58. A therapeutic ketogenic diet was associated with significant cognitive improvement in this patient with concurrent Down syndrome and dementia.
Predictors of psychological stress occurring after the first wave of the COVID-19 pandemic in Poland: A cross-sectional study
Authors: Długosz Piotr
Published: January 2023

The article presents the results of research aimed to identify the predictors of psychological distress among Poles 7 months after the occurrence of the first case of COVID-19. In order to gather the research material, the CAWI on-line survey method was applied and carried out within the framework of the Ariadna Research Panel on the sample of 1,079 Poles aged 15 and over. The results of the conducted research indicate that Polish society experienced psychological distress during the first wave of the pandemic. According to the Kessler Psychological Distress Scale (K10), no mental disorders were observed among 36% of Poles, mild mental disorders were observed among 23% of respondents, average levels of disorders were observed among 18% of respondents, whereas high levels of disorders were observed among 23% of respondents. A hierarchical linear regression analysis was used to identify the risk factors of psychological distress. In the first stage, socio-demographic variables explained 13% of the distress variance. In the second stage, the variables measuring social nuisances of the pandemic were introduced, which increased the percentage of the explained stress variance to 24%. In the third stage, the introduced psychological variables increased the percentage of the explained variance to 65%. The main factor which increased stress levels was neuroticism. The conducted analyses have shown that the lack of social, economic and psychological capital significantly increases the susceptibility to distress when a threat to life and health lasts for a prolonged period of time.
Alcohol consumption patterns of the Hungarian general and Roma populations
Authors: Kurshed Ali Abbas Mohammad , Vincze Ferenc , Pikó Péter , Kósa Zsigmond , Sándor János , Ádány Róza , Diószegi Judit
Published: January 2023

Introduction

Harmful alcohol use is a significant public health problem worldwide, though the alcohol-related burden affects disproportionately certain populations and ethnic minorities, with the WHO European Region being the most heavily affected and putting an increased risk on Roma populations. This ethnic minority group is the largest and most vulnerable ethnic minority in Europe and Hungary as well.

Methods

The present study aims to describe and compare the alcohol consumption behaviors of the Hungarian general and Roma populations using the Alcohol Use Disorders Identification Test (AUDIT), which provides a comprehensive view of alcohol consumption behavior. In addition, a decomposition analysis was performed when the multivariate logistic or Poisson regression model showed significant differences between the two samples.

Results

Our findings suggest that Roma people in our study sample experience more alcohol-related harm, even when considering past problems. The decomposition analysis revealed that gender and relationship status differences act more intensely among Roma than non-Roma when considering alcohol-related harm.

Discussion

Equalizing these differences would be expected to reduce the Hungarian general and Roma populations' alcohol-related harm frequency gap. Investigating alcohol-attributed harms at the ethnicity level provides important information to identify high-risk groups and, thus, to design and implement more targeted and accessible interventions for alcohol problems.
Exploring Italian healthcare facilities response to COVID-19 pandemic: Lessons learned from the Italian Response to COVID-19 initiative
Authors: Parotto Emanuela , Lamberti-Castronuovo Alessandro , Censi Veronica , Valente Martina , Atzori Andrea , Ragazzoni Luca
Published: January 2023

The COVID-19 pandemic exerted an extraordinary pressure on the Italian healthcare system (Sistema Sanitario Nazionale, SSN), determining an unprecedented health crisis. In this context, a multidisciplinary non-governmental initiative called Italian Response to COVID-19 (IRC-19) was implemented from June 2020 to August 2021 to support the Italian health system through multiple activities aimed to mitigate the effects of the pandemic. The objective of this study was to shed light on the role of NGOs in supporting the SSN during the first pandemic wave by specifically exploring: (1) the main challenges experienced by Italian hospitals and out-of-hospital care facilities and (2) the nature and extent of the IRC-19 interventions specifically implemented to support healthcare facilities, to find out if and how such interventions met healthcare facilities' perceived needs at the beginning of the pandemic. We conducted a cross-sectional study using an interviewer administered 32-item questionnaire among 14 Italian healthcare facilities involved in the IRC-19 initiative. Health facilities' main challenges concerned three main areas: healthcare workers, patients, and facilities' structural changes. The IRC-19 initiative contributed to support both hospital and out-of-hospital healthcare facilities by implementing interventions for staff and patients' safety and flow management and interventions focused on the humanization of care. The support from the third sector emerged as an added value that strengthened the Italian response to the COVID-19 pandemic. This is in line with the Health—Emergency and Disaster Risk Management (H-EDRM) precepts, that call for a multisectoral and multidisciplinary collaboration for an effective disaster management.
Factors associated with the mental health status of pregnant women in China: A latent class analysis
Authors: Pei Yifei , Chen Qian , Zhang Ying , He Chenlu , Wang Jingjing , Tang Jie , Hou Hao , Zhu Ziqing , Zhang Xunbao , Wang Wei
Published: January 2023

Background

Prenatal mental health is a neglected public health issue that places pregnant women at a higher risk for mental disorders. The purpose of this study was to investigate the influencing factors of prenatal mental disorders and provide a scientific basis to guide and promote the mental health of pregnant women.

Methods

The study sample comprised 973 women in their first pregnancy, who were in their second trimester and third trimester, who underwent obstetric outpatient checkups at the Maternal and Child Health Hospital in Huai'an, who were recruited in the survey that was conducted from July to December 2017. The Chinese mental health scale (CMHS) was used to assess the mental health of pregnant women. The present study uses the chi-square test to compare the rates of class with different demographic variables, a latent class analysis to identify psychological symptoms, and multiple logistic regression analysis to examine whether the demographics predicted class membership.

Results

The chi-square test results showed that participants who reported feeling different in the perinatal period (χ2 = 6.35, P = 0.04), having marital satisfaction (χ2 = 15.8, P < 0.001), with an in-law relationship (χ2 = 29.43, P < 0.001), with a friend relationship (χ2 = 24.81, P < 0.001), with basic diseases (χ2 = 8.04, P = 0.02), and taking birth control pills (χ2 = 8.97, P = 0.01) have different probabilities of being classified. Three latent classes were identified: the high symptoms group (6.89%), the moderate symptoms group (20.56%), and the low symptoms group (72.56%). Pregnant women in the third trimester [odds ratio (OR) = 1.83, 95% confidence interval (CI): 1.04–3.25, P = 0.04], with a poor in-law relationship (OR = 2.82, 95% CI:1.45–5.51, P = 0.002), with a bad friend relationship (OR = 3.17, 95% CI: 1.31–7.71, P = 0.01), and who had basic diseases (OR = 1.70, 95% CI: 1.00–2.90, P = 0.04) tended to be classified under the high symptoms group than under the low symptoms group. Pregnant women with a bad friend relationship (OR = 2.15, 95% CI: 1.08–4.28, P = 0.03) and taking birth control pills (OR = 1.51, 95% CI: 1.08–2.11, P = 0.02) were more likely to be placed under the moderate symptoms group than under the low symptoms group.

Conclusions

A pregnant woman's mental health status factors include feeling different in the perinatal period, those with marital satisfaction, those with an in-law relationship, those with a friend relationship, those with basic diseases, and those taking birth control pills. To ensure a smooth progress of pregnancy and promote the physical and mental health of pregnant women, psychological screening and psychological intervention measures should be strengthened.
Prevalence of and risk factors associated with depression among nursing students acting on the frontline of COVID-19 pandemic: A cross-sectional study
Authors: Nguyen Huyen Thi Hoa , Hoang Anh Phuong , Vu Linh Manh , Tran Duc Quang , Bui Linh Khanh , Pham Thuan Thi , Tran Linh Thuy Khanh , Nguyen Huong Thi Thanh
Published: January 2023

Introduction

The widespread devastation caused by the ongoing waves of COVID-19 imposed a significant burden on the healthcare labor force. At the frontline in the battle against the deadly COVID-19 virus, nursing students in Vietnam were at a much-increased risk of developing mental health conditions. This study aims to identify the prevalence of depression and its related factors, along with coping strategies used by nursing students in the COVID-19 pandemic in Vietnam.

Materials and methods

The study was cross-sectional in nature, with convenient sampling at the epicenters of COVID-19 outbreaks in Vietnam (N = 191) from April to November 2021. After conducting a questionnaire pilot, the data was collected strictly using an internet-based approach. The Depression, Anxiety, and Stress Scale-21 items were used to identify the risk of depression among nursing students. The Chi-square test was used to assess the differences between coping strategies among nursing students. A multivariate logistic regression model was used to identify risk factors associated with depression.

Findings

The percentage of nursing students affected by depression was 21.5%, and almost half of the nursing students (49.2%) had no coping strategies for dealing with mental health concerns. Among the remaining nursing students, video-based mental consultation was the most popular method (25.7%). Being females (AOR: 2.7, 95% CI: 1.1–6.7), collecting bio-samples (AOR: 2.9, 95% CI: 1.4–6.2), providing support to vaccination spots (AOR: 2.3, 95% CI: 1.1–5.1), and not vaccinating against COVID-19 (AOR: 3.1, 95% CI: 1.1–9.1) were found as risk factors for depression among nursing students.

Conclusion

Our research revealed a significant number of nursing students suffering from depressive symptoms and underscoring the need for more effective methods of dealing with this condition. Depression management and coping skills focusing on female populations and those whose direct contacts with infectious sources should be implemented in the nursing curricula and continuous training credits. Those trainings, would support future nurses in handling crisis situations better.
A comparative study of ultrasound-guided puncture biopsy combined with histopathology and Xpert MTB/RIF in the diagnosis of lymph node tuberculosis
Authors: Meng Xiangyu , Fu Hongxiang , Jia Weina , Wang Ying , Yang Gaoyi
Published: January 2023

Background

Cervical tuberculous lymphadenitis (CTBL) is a disease often ignored in clinical work, and pathology and Xpert MTB/RIF (Xpert) are the commonly used methods for tuberculosis diagnosis. This study aimed to compare ultrasound-guided puncture biopsy combined with histopathology and Xpert in the diagnosis of lymph node tuberculosis.

Methods

A total of 217 patients highly suspected for CTBL were retrospectively enrolled. All patients underwent ultrasound-guided puncture sampling. All samples were subjected to pathological examination and Xpert test. The sensitivity and specificity of the two methods were compared for all samples. The kappa value was calculated to assess the consistency of the pathological examination and Xpert test using comprehensive diagnosis as the gold standard. Receiver operating characteristic curves of the pathological examination, Xpert test, and their combination were generated, and the areas under the curve (AUCs) were calculated to compare the diagnostic value of the three methods.

Results

The sensitivity and specificity of the pathological diagnosis of CTBL were 70.1 and 100%, respectively. The sensitivity and specificity of Xpert for CTBL diagnosis were 82.5 and 97.5%, respectively. The results of the pathological examination and Xpert test showed poor consistency in the diagnosis of CTBL, with a kappa value of 0.388. The AUC of the pathological diagnosis of CTBL was 0.850 (95% CI: 0.796–0.895), whereas that of Xpert was 0.900 (95% CI: 0.852–0.936), and the difference was statistically significant (P = 0.0483). The AUC of pathological examination combined with Xpert for the diagnosis of CTBL was 0.956 (95% CI: 0.920–0.979), and the difference between pathological examination combined with Xpert for the diagnosis of CTBL was statistically significant compared with pathological examination and Xpert alone, respectively (both P < 0.001).

Conclusion

The diagnostic efficiency of Xpert test is higher than that of pathological examination, but its sensitivity is still not ideal for clinical diagnosis. According to this study, the consistency of Xpert test and pathological diagnosis is poor, and the combination of Xpert test and pathological diagnosis can significantly increase the diagnostic efficiency.
High risk of unsuccessful treatment outcome in migrant population with tuberculosis: Data from three Italian hospitals
Authors: Di Gennaro Francesco , Cotugno Sergio , Fasano Massimo , Ricciardi Aurelia , Ronga Luigi , Lattanzio Rossana , Grimaldi Anna , Bavaro Davide Fiore , Ciarallo Marianna , Garzone Stefania , De Iaco Giuseppina , Guido Giacomo , Fiore Josè Ramon , Brindicci Gaetano , Santoro Carmen Rita , Sica Salvatore , Iacovazzi Tiziana Loredana , Santantonio Teresa Antonia , Saracino Annalisa
Published: January 2023

Introduction

Tuberculosis (TB) remains an unresolved global health problem and vulnerable groups such as migrants remain the most affected with a higher risk of worse outcomes. The aim of this study was to evaluate clinical features, outcomes, and adverse events in migrant and native Italian patients admitted to three Italian hospitals in Southern Italy in order to assess differences and targeted strategies.

Methods

We performed a retrospective study on TB patients admitted between January 1, 2013, and December 31, 2021, in three Apulia hospitals. Two logistic regression models were used, with the dependent variables being (I) unsuccessful treatment (died, loss to follow-up, and failed treatment) and (II) adverse events.

Results

We enrolled 543 consecutive patients admitted at three Italian hospitals with a diagnosis of TB during the study period, of them 323 (59.5%) were migrants and 220 Italian patients. The treatment success rate in the migrant group was 44.9% (137/305), while in the non-migrant group was 97.1% (203/209). Independent factors of unsuccess treatment (death, failure or loss to follow up) were: migrant status (O.R. = 11.31; 95% CI 9.72–14.23), being male (O.R. = 4.63; 95% CI 2.16–6.10), homelessness (O.R. = 3.23; 95% CI 2.58–4.54), having a MDR (Multidrug-resistant) (O.R = 6.44; 95% CI 4.74–8.23), diagnostic delay (O.R. = 3.55; 95% CI 1.98–5.67), and length of hospitalization (O.R. = 3.43; 95% CI 1.88–5.87). While, age >65 ys (O.R. = 3.11; 95% CI 1.42–4.76), presence of extrapulmonary TB (O.R. = 1.51; 95% CI 1.31–2.18), monoresistance (O.R. = 1.45; 95% CI 1.25–3.14) and MDR pattern (O.R. = 2.44; 95% CI 1.74–5.03) resulted associated with adverse events.

Conclusion

Migrant population is at high risk of unsuccessful treatment (death, loss to follow-up, and treatment failure). Policies targeted specifically to this group are needed to really impact and improve their health status and also to contain the TB burden.
The North Italian innovative project for common psychiatric disorders: Evaluating the output of a treatment model of an outpatient clinic for anxiety and depression
Authors: Caselli Ivano , De Leo Alessandra , Isella Celeste , Bellini Alessandro , Ielmini Marta , Callegari Camilla
Published: January 2023

Depressive disorders were considered the first causes of disability worldwide as early as 2018. The outpatient clinic for anxiety and depression at the University Hospital of Varese represents a service that fully responds to the growing number of requests. Approximately 1,350 medical records have been opened from 2010 to December 2021. The most frequent presenting diagnoses included anxiety disorders (36.8%), severe stress and maladaptation syndromes (35.5%), and depressive episodes (18%). The outpatient clinic has proved to be a model with great impact on users offering a range of diagnostic and therapeutic offers responding to the requests of the community.
Global burden of lower respiratory infections during the last three decades
Authors: Safiri Saeid , Mahmoodpoor Ata , Kolahi Ali-Asghar , Nejadghaderi Seyed Aria , Sullman Mark J. M. , Mansournia Mohammad Ali , Ansarin Khalil , Collins Gary S. , Kaufman Jay S. , Abdollahi Morteza
Published: January 2023

Background

Lower respiratory infections (LRIs) cause a substantial mortality, morbidity and economic burden. The present study reported the global, regional and national burden of LRIs and their attributable risk factors in 204 countries and territories, between 1990 and 2019, by age, sex, etiology, and Socio-demographic Index (SDI).

Methods

Using publicly available data from the Global Burden of Disease (GBD) study 2019, we reported the incidence, deaths and disability-adjusted life-years (DALYs), due to LRIs. Estimates were presented as counts and age-standardized rates per 100,000 population with their associated uncertainty intervals (UIs).

Results

Globally, in 2019 there were 488.9 million (95% UI: 457.6 to 522.6) incident cases and 2.4 million (2.3–2.7) deaths due to LRIs. The global age-standardized incidence and death rates for LRIs were 6,295 (5,887.4–6,737.3) and 34.3 (31.1–37.9) per 100,000 in 2019, which represents a 23.9% (22.5–25.4) and 48.5% (42.9–54.0) decrease, respectively since 1990. In 2019, Guinea [12,390.4 (11,495.5–13,332.8)], Chad [12,208.1 (11,289.3–13,202.5)] and India [11,862.1 (11,087.0–12,749.0)] had the three highest age-standardized incidence rates of LRI. Equatorial Guinea [−52.7% (95% UI: −55.8 to −49.3)], Chile [−50.2% (95% UI: −53.4 to −47.0)] and Albania [−48.6% (95% UI: −51.7 to −45.3)] showed the largest decreases from 1990 to 2019. In 2019, a decrease in the incidence rate of LRI was observed at the global level up to the 25–29 age group, then the incidence rates increased with age. The burden of LRIs decreased with increasing SDI at both the regional and national levels. Globally, child wasting (33.1%), household air pollution from solid fuels (24.9%) and a lack of access to handwashing facilities (14.4%) made the largest contributions to the LRI burden in 2019.

Conclusions

Although the burden of LRIs decreased over the period 1990–2019, LRIs still contribute to a large number of incident cases, deaths and DALYs. Preventative programs with a focus on reducing exposure to attributable risk factors should be implemented, especially in less developed countries.
Omicron subvariant BA.5 is highly contagious but containable: Successful experience from Macau
Authors: Xu Cong , Wang Jue , Yu Lili , Sui Xinbing , Wu Qibiao
Published: January 2023

Introduction

Due to its high transmissibility and immune escape, Omicron subvariant BA.5 has become the dominant strain of the SARS-CoV-2 virus and led to escalating COVID-19 cases, how to cope with it becomes an urgent issue. A BA.5 infection surge burst out on 18 June 2022 and brought an unprecedented challenge to Macau, the most densely populated region worldwide. This study aimed to analyze the characteristics of this outbreak and summarize the useful anti-epidemic measures and experiences during this outbreak.

Methods

All data were obtained from the Government Portal of Macao SAR (https://www.gov.mo), and the Special Webpage Against Epidemics, the Macao Health Bureau (www.ssm.gov.mo). An epidemiologic study was performed to analyze epidemic outcomes, including the infection rate, the proportion of symptomatic cases, the case fatality ratio (CFR), etc. Data were analyzed using SPSS Version 20. A p-value <0.05 was considered statistically significant. The anti-epidemic measures and experience were reviewed and summarized.

Results

The BA.5 outbreak resulted in 1,821 new cases, which was significantly more than the cumulative cases of the previous variants of COVID-19 in Macau. The symptomatic cases accounted for 38.71% of the total cases, which was higher than that of the previous variants. After 6-week concerted efforts, Macau effectively controlled the outbreak, with an infection rate of 0.27%, which was much lower than many BA.5-attacked regions. The CFR was approximately 0.86%, which was not statistically different from that of previous variants. Six victims were chronically ill senior elders and their vaccination rate was much lower than the average level. Macau took a comprehensive anti-epidemic strategy to win a quick victory against BA.5, especially the “relatively static” strategy that was first formulated and applied by Macau for the management of the COVID-19 pandemic. Successful experience showed that although BA.5 was highly contagious, it could be contained by comprehensive anti-epidemic measures, including adequate anti-epidemic preparation, herd immunity through vaccination, repeated mass nucleic acid tests and rapid antigen tests, KN-95 mask mandate, the “relatively static” strategy, precise prevention and control, epidemiological investigation and tracing, and traditional Chinese medicine treatment, etc.

Discussion

In Macau, compared with the previous subvariants, BA.5 is associated with increased transmissibility and a higher proportion of symptomatic cases, however, the risk of death remains similar, and the infection rate is much lower than that in many other BA.5-attacked regions. BA.5 is highly contagious but still containable, Macau's experience may offer hints for the regions experiencing the BA.5 waves to choose or adjust a more rational anti-epidemic strategy.
Associations between work-related variables and workplace violence among Chinese medical staff: A comparison between physical and verbal violence
Authors: Sun Long , Zhang Wen , Cao Aihua
Published: January 2023

Background

Workplace violence (WPV) against medical staff has been an important public health and societal problem worldwide. Although numerous studies have implied the differences between physical violence (PV) and verbal violence (VV) against medical staff, few studies were conducted to analyze the different associations between work-related variables, PV, and VV, especially in China.

Methods

A cross-sectional study was conducted among Chinese medical staff in public hospitals, and 3,426 medical staff were interviewed and analyzed. WPV, including PV and VV, were evaluated by the self-report of the medical staff. Work-related variables, physical disease, depression, and social-demographic variables were also measured. The work-related variables included types of medical staff, professional titles, hospital levels, managers, working years, job changing, working hours/week, night duty times/week, monthly income, self-reported working environment, and social position. Logistic regressions were conducted to examine the factors associated with PV and VV.

Results

A total of 489 medical staff (23.0%) reported the experience of PV and 1,744 (50.9%) reported the experience of VV. Several work-related variables were associated with PV and VV, including nurse (OR = 0.56 for PV, p < 0.01; OR = 0.76 for VV, p < 0.05), manager (OR = 1.86 for PV, p < 0.01; OR = 1.56 for VV, p < 0.001), night duty frequency/week (OR = 1.06 for PV, p < 0.01; OR = 1.03 for VV, p < 0.01), bad working environment (OR = 2.73 for PV, p < 0.001; OR = 3.52 for VV, p < 0.001), averaged working environment (OR = 1.51 for PV, p < 0.05; OR = 1.55 for VV, p < 0.001), and bad social position (OR = 4.21 for PV, p < 0.001; OR = 3.32 for VV, p < 0.001). Working years (OR = 1.02, p < 0.05), job changing (OR = 1.33, p < 0.05), and L2 income level (OR = 1.33, p < 0.01) were positively associated with VV, but the associations were not supported for PV (all p>0.05). The other associated factors were male gender (OR = 1.97 for PV, p < 0.001; OR = 1.28 for VV, p < 0.05) and depression (OR = 1.05 for PV, p < 0.001; OR = 1.04 for VV, p < 0.001).

Conclusion

Both PV and VV were positively associated with work-related variables, such as doctor, manager, more night duty frequency, perceived bad working environment, or social position. Some variables were only associated with VV, such as working years, job changing, and monthly income. Some special strategies for the work-related variables should be applied for controlling PV and VV.
A national cross-sectional analysis of selenium intake and risk of osteoarthritis: NHANES 2003–2016
Authors: Deng Xiaoyu , Tan Yongqiong
Published: January 2023

Background

The association between dietary selenium intake and arthritis, rheumatoid arthritis (RA), and osteoarthritis (OA) is inconsistent in previous studies and remain unclear. To investigate their relationship, this study was performed.

Methods

Data from the National Health and Nutrition Examination Survey (2003–2016) were downloaded and further analyzed. Dietary Se intake was classified according to quartiles with quartile 1 (Q1) having the lowest intake and quartile 4 (Q4) having the highest intake. Weighted logistic regression was used to investigate the association between dietary selenium intake and arthritis, RA, and OA. Subgroup analyses were performed to verify the findings. To further examine the non-linear relationship between dietary selenium intake and OA, restricted cubic spline (RCS) was adopted.

Results

In the crude model, the highest level of dietary selenium intake was siginificantly associated with decreased risks of arthritis (OR: 0.40, 95% CI: 0.37, 0.44) and rheumatoid arthritis (OR: 0.47, 95% CI: 0.40, 0.54), respectively. In the fully adjusted model, dietary selenium intake was not associated with risk of arthritis and RA (all P > 0.05). Conversely, the risk of OA was noted for participants with higher selenium intake (odds ratio of quartile 4 = 1.33, 95% CI = 1.07–1.65, P < 0.05). In the subgroup analyses, participants with diabetes had a higher risk of OA when ingested high selenium levels than those without diabetes (P < 0.001). The results of RCS showed that significant overall trends were found between dietary selenium intake and osteoarthritis (P for overall < 0.05). However, non-linear association was not detected in this association (P for non-linear > 0.05).

Conclusion

Using data from NHANES, this study discloses that high dietary selenium intake might be associated with risk of OA. However, the generalization of conclusion needs further examination because of the limitation of dietary questionnaire survey.