bluuebunny/crossref_metadata_2025 huggingface dataset:
A Crucial Role for the Small GTPase Rac1 Downstream of the Protein Kinase Akt2 in Insulin Signaling that Regulates Glucose Uptake in Mouse Adipocytes
Authors: Takenaka Nobuyuki , Nakao Mika , Matsui Sayaka , Satoh Takaya
Published: October 2019

Insulin-stimulated glucose uptake is mediated by translocation of the glucose transporter GLUT4 to the plasma membrane in adipocytes and skeletal muscle cells. In both types of cells, phosphoinositide 3-kinase and the protein kinase Akt2 have been implicated as critical regulators. In skeletal muscle, the small GTPase Rac1 plays an important role downstream of Akt2 in the regulation of insulin-stimulated glucose uptake. However, the role for Rac1 in adipocytes remains controversial. Here, we show that Rac1 is required for insulin-dependent GLUT4 translocation also in adipocytes. A Rac1-specific inhibitor almost completely suppressed GLUT4 translocation induced by insulin or a constitutively activated mutant of phosphoinositide 3-kinase or Akt2. Constitutively activated Rac1 also enhanced GLUT4 translocation. Insulin-induced, but not constitutively activated Rac1-induced, GLUT4 translocation was abrogated by inhibition of phosphoinositide 3-kinase or Akt2. On the other hand, constitutively activated Akt2 caused Rac1 activation, and insulin-induced Rac1 activation was suppressed by an Akt2-specific inhibitor. Moreover, GLUT4 translocation induced by a constitutively activated mutant of Akt2 or Rac1 was diminished by knockdown of another small GTPase RalA. RalA was activated by a constitutively activated mutant of Akt2 or Rac1, and insulin-induced RalA activation was suppressed by an Akt2- or Rac1-specific inhibitor. Collectively, these results suggest that Rac1 plays an important role in the regulation of insulin-dependent GLUT4 translocation downstream of Akt2, leading to RalA activation in adipocytes.
Conjunctival Melanoma: Genetic and Epigenetic Insights of a Distinct Type of Melanoma
Authors: Rossi Ernesto , Schinzari Giovanni , Maiorano Brigida Anna , Pagliara Monica Maria , Di Stefani Alessandro , Bria Emilio , Peris Ketty , Blasi Maria Antonietta , Tortora Giampaolo
Published: October 2019

Conjunctival melanoma (CjM) is a rare, primary cancer of the ocular region. Genetic and epigenetic characteristics of conjunctival melanoma have not been completely elucidated yet. Conjunctival melanoma presents similarities with cutaneous melanoma, with substantial differences in the biological behavior. We reviewed the genetic and epigenetic insights of CjM involved in invasion and metastatic spread. CjM is commonly characterized by mutations of v-raf murine sarcoma viral oncogene homolog B1 (BRAF), neurofibromin 1 (NF1) and telomerase reverse transcriptase (TERT), high expression of mammalian target of rapamycin (mTOR) and heat shock protein 90 (HSP90), frequent phosphatase and tensin homolog (PTEN) loss and upregulation of specific miRNAs. These features should identify CjM as a distinct subset of melanoma with its own profile, which is more similar to cutaneous melanoma than mucosal melanoma and remarkably different from uveal melanoma.
Optimization of Mature Embryo-Based Tissue Culture and Agrobacterium-Mediated Transformation in Model Grass Brachypodium distachyon
Authors: Yu Guangrun , Wang Jianyong , Miao Li , Xi Mengli , Wang Qiongli , Wang Kai
Published: October 2019

Agrobacterium-mediated genetic transformation is well established in the model grass Brachypodium distachyon. However, most protocols employ immature embryos because of their better regenerative capacity. A major problem associated with the immature embryo system is that they are available only during a limited time window of growing plants. In this study, we have developed an optimized Agrobacterium-mediated genetic transformation protocol that utilizes mature embryos. We have adopted seed shearing and photoautotrophic rooting (PR) in callus induction and root regeneration, respectively, with evident significant improvement in these aspects. We have also revealed that the newly developed chemical inducer Fipexide (FPX) had the ability to induce callus, shoots, and roots. By comparison, we have demonstrated that FPX shows higher efficiency in shoot generation than other frequently used chemicals in our mature embryo-based system. In addition, we demonstrated that the age of embryogenetic callus severely affects the transformation efficiency (TE), with the seven-week-old embryogenetic callus having the highest TE reaching 52.6%, which is comparable with that in immature embryo transformation. The new methodologies reported here will advance the development and utilization of Brachypodium as a new model system for grass genomics.
Susceptibility of Environmentally Friendly Sheep Wool Insulation Panels to the Common Clothes Moth Tineola bisselliella in Laboratory Assays
Authors: Ruiu Luca , Floris Ignazio
Published: October 2019

In this study the resistance opposed to Tineola bisselliella larvae by a commercial sheep-wool panel incorporating borate salts was determined under laboratory conditions. The susceptibility of clothes moth larvae to different concentrations of disodium octaborate tetrahydrate (DOT) incorporated in pure wool was also determined. The commercial wool panel showed a remarkable resistance to moth attack compared with pure untreated wool, and the damage to panel samples was limited to their surface. As a result of bioassays exposing larvae to pure wool treated with DOT, a concentration dependent effect was observed, achieving a good efficacy at an application rate between 40–100 mg/mL. This study highlights the need to protect wool-based construction material with appropriate insecticidal (antifeedant or repellent) substances and supports the development of eco-sustainable solutions.
A Survey of Scrutinizing Delaminated Composites via Various Categories of Sensing Apparatus
Authors: Askaripour Khadijeh , Zak Arkadiusz
Published: October 2019

Scrutinizing delaminated composites is extensively conducted based on measurements of various types of nondestructive sensing apparatus, classified herein as systems of thermographic inspection, piezoelectric inspection, optical interferometric inspection, electromagnetic testing, ultrasonic testing, and visual testing. The development of the subject has been reviewed during 2000–2017. Not only does the substantial corresponding literature strongly indicates numerous increasing industrial demand for composites, including carbon fiber-reinforced polymer, glass fiber-reinforced polymer, and fiber-reinforced metal laminate, but also the significant potential of composites to delaminate when subjected particularly to impact, machining operation or manufacturing imperfections. Conducting a study on the corresponding literature, the subject of delamination inspection within composites is found to be a dynamic, mature field, while lacking a thorough literature review. Therefore, the present study addresses the mentioned gap regarding various sensation systems utilized so as to detect internal anomalies in composites for the review arrangement. To that end, the literature inclusion in terms of citation times is electronically adopted associated with an appropriate combination of keywords. The study may be considered as a comprehensive, up-to-date review covering all delamination inspection techniques founded on sensation systems, thus benefiting the readers with information in an organized configuration.
Study on Propulsion Performance by Varying Rake Distribution at the Propeller Tip
Authors: Kang Jin Gu , Kim Moon Chan , Kim Hyeon Ung , Shin I. Rok
Published: October 2019

This study provides a comparison of propulsion performance, with a particular focus on efficiency, by varying rake distribution at the tips of propellers. Owing to increased attention to environmental pollution, there is a significant interest in reducing the energy efficiency design index (EEDI) and SOx emissions by improving the performance in the field of shipbuilding. The forward (Kappel) and backward tip rake propellers have been widely used to improve efficiency, as well as to reduce fluctuating pressure from the tip vortex cavitation. As there is almost no parametric and design research on tip rake propellers, this systematic parametric study was conducted to identify the optimal configuration by the potential code. For this performance comparison the KP505 (KCS propeller) was chosen as the reference propeller as the tips of that propeller have no rake. The model test and computational fluid dynamics (CFD) calculation confirmed the result by comparing the open water performances for the three optimally selected propellers (forward, backward, KP505). The differences of efficiency obtained from the potential analysis and the model test exhibit similar tendencies, but the result for the CFD is different. The difference would be investigated by changing the grid system around the tip as well as the turbulence model in the CFD analysis. An analysis of self-propulsion and pressure fluctuation is also expected to be conducted in the near future.
Coral Reef Resilience in Taiwan: Lessons from Long-Term Ecological Research on the Coral Reefs of Kenting National Park (Taiwan)
Authors: Keshavmurthy Shashank , Kuo Chao-Yang , Huang Ya-Yi , Carballo-Bolaños Rodrigo , Meng Pei-Jei , Wang Jih-Terng , Chen Chaolun Allen
Published: October 2019

Coral reefs in the Anthropocene are being subjected to unprecedented levels of stressors, including local disturbances—such as overfishing, habitat destruction, and pollution—and large-scale destruction related to the global impacts of climate change—such as typhoons and coral bleaching. Thus, the future of corals and coral reefs in any given community and coral-Symbiodiniaceae associations over time will depend on their level of resilience, from individual corals to entire ecosystems. Herein we review the environmental settings and long-term ecological research on coral reefs, based on both coral resilience and space, in Kenting National Park (KNP), Hengchun Peninsula, southern Taiwan, wherein fringing reefs have developed along the coast of both capes and a semi-closed bay, known as Nanwan, within the peninsula. These reefs are influenced by a branch of Kuroshio Current, the monsoon-induced South China Sea Surface Current, and a tide-induced upwelling that not only shapes coral communities, but also reduces the seawater temperature and creates fluctuating thermal environments which over time have favoured thermal-resistant corals, particularly those corals close to the thermal effluent of a nuclear power plant in the west Nanwan. Although living coral cover (LCC) has fluctuated through time in concordance with major typhoons and coral bleaching between 1986 and 2019, spatial heterogeneity in LCC recovery has been detected, suggesting that coral reef resilience is variable among subregions in KNP. In addition, corals exposed to progressively warmer and fluctuating thermal environments show not only a dominance of associated, thermally-tolerant Durusdinium spp. but also the ability to shuffle their symbiont communities in response to seasonal variations in seawater temperature without bleaching. We demonstrate that coral reefs in a small geographical range with unique environmental settings and ecological characteristics, such as the KNP reef, may be resilient to bleaching and deserve novel conservation efforts. Thus, this review calls for conservation efforts that use resilience-based management programs to reduce local stresses and meet the challenge of climate change.
Social and Financial Inclusion through Nonbanking Institutions: A Model for Rural Romania
Authors: Yue Xiao-Guang , Cao Yong , Duarte Nelson , Shao Xue-Feng , Manta Otilia
Published: October 2019

The challenges of financial systems have immediate or medium-term social effects. The financial industry is constantly searching for measures to reduce these challenges, especially for those with little or no access to financial services. While current communication technologies make services more accessible through digital mobile platforms, there are still difficulties in establishing viable customer arrangements. In addition to the increased investment in financial technologies, nonbanking financial institutions have now expanded to offer more flexible services tailored to individual circumstances, especially those in isolated rural areas. This research outlines the network model of nonbanking financial institutions in Romania, as well as a microfinance model, based on the financial analysis of four national indicators of nonbanking financial institutions. Data used are presented in absolute values, from the annual numerical series for the reference period 2007–2017. The new initiatives and features incorporated in this Romanian model should be applicable elsewhere and will actively contribute to the expansion and sustainability of financial services, with a positive inclusive impact on society.
Circular Economy and Waste in the Fashion Industry
Authors: Jacometti Valentina
Published: October 2019

The fashion industry has to play an important role in the path towards sustainability and the circular economy. Indeed, the fashion industry is a sector with a high environmental impact; it involves a very long and complicated supply chain, which is associated with large consumption of water and energy, use of chemical substances, water and air pollution, waste production and finally microplastic generation. In particular, textiles and clothing waste has become a huge global concern. Against this background, this paper aims at analysing the existing EU measures that have an impact on the development of sustainable practices and the transition to a circular economy in the fashion industry, with a particular focus on the EU revised legislative framework on waste adopted within the Circular Economy Action Plan of 2015.
A Comparative Study of Shock Absorption Capacities of Custom Fabricated Mouthguards Using a Triangulation Sensor
Authors: Sarac Rūta , Helbig Julia , Dräger Juliane , Jost-Brinkmann Paul-Georg
Published: October 2019

This in-vitro study compares the shock absorption qualities of five mouthguard designs measured with a triangulation laser sensor during small hard object collisions. The aim was to investigate the impact of different labial designs on mouthguard performance. Methods: Five different custom-fabricated ethylene vinyl acetate (EVA) types of mouthguards with varying thickness and different labial inserts (polyethylene terephthalate glycol-modified (PETG), nylon mesh, air space) were tested with a triangulation laser sensor during different energy blows, generated with a pendulum testing device. The pendulum hits were applied to the center of a pivoted tooth crown in a custom-built upper jaw model. Measurements were executed with the mouthguards on the model and with no mouthguard as a negative control. Results: Tooth deflection was reduced with all mouthguards in comparison to no mouthguard. Increasing mouthguard thickness improved the mouthguards’ shock absorption capacities. Also, adding labial inserts increased their preventive qualities in ascending order: Mouthguard with a soft insert (nylon mesh), a hard insert (PETG), air space plus a hard insert (PETG). Conclusion: Increasing EVA foil thickness of a mouthguard, increasing labial thickness, and adding labial inserts (soft, stiff and air space) improve mouthguard shock absorption capabilities during small hard object collisions, thereby improving dental trauma prevention.
Buckling and Post-Buckling Behavior of Uniform and Variable-Density Lattice Columns Fabricated Using Additive Manufacturing
Authors: Nazir Aamer , Arshad Ahmad Bin , Jeng Jeng-Ywan
Published: October 2019

Lattice structures are known for their high strength-to-weight ratio, multiple functionalities, lightweight, stiffness, and energy absorption capabilities and potential applications in aerospace, automobile, and biomedical industry. To reveal the buckling (global and local) and post-buckling behavior of different lattice morphologies, both experimental and simulation-based studies were carried out. Additionally, a variable-density lattice structure was designed and analyzed to achieve the optimal value of critical buckling load. Latticed columns were fabricated using polyamide 12 material on multi jet fusion 3D printer. The results exhibited that the buckling in lattice columns depends on the distribution of mass, second moment of inertia I, diameter and position of vertical beams, number of horizontal or inclined beams, and location and angle of the beams that support the vertical beams. The number of horizontal and inclined beams and their thickness has an inverse relation with buckling; however, this trend changes after approaching a critical point. It is revealed that vertical beams are more crucial for buckling case, when compared with horizontal or inclined beams; however, material distribution in inclined or horizontal orientation is also critical because they provide support to vertical beams to behave as a single body to bear the buckling load. The results also revealed that the critical buckling load could be increased by designing variable density cellular columns in which the beams at the outer edges of the column are thicker compared with inner beams. However, post-buckling behavior of variable density structures is brittle and local when compared with uniform density lattice structures.
Improved DC Dielectric Performance of Photon-Initiated Crosslinking Polyethylene with TMPTMA Auxiliary Agent
Authors: Qiu Peng , Chen Jun-Qi , Sun Wei-Feng , Zhao Hong
Published: October 2019

To achieve high direct current (DC) dielectric performance of crosslinked polyethylene (XLPE) applied for insulated cable, the auxiliary crosslinking agent of trimethylolpropane trimethacrylate (TMPTMA) is employed in photon-initiated crosslinking process to the present polar-molecular group which will introduce deep traps for charge carriers. The space-charge accumulation and electrical conductance of XLPE are observably suppressed due to the deep traps deriving from the TMPTMA crosslinkers that are chemically connecting (grafted onto) polyethylene molecules. Thermally stimulated depolarization current tests and first-principles calculations consistently demonstrate a trapping mechanism of impeding charge injection and carrier transport in XLPE with TMPTMA crosslinkers. The characteristic cyclic anhydrides with coupled carbonyl groups are used as auxiliary crosslinkers to promote crosslinking efficiency and provide polar groups to polyethylene molecules which can be effectively fulfilled in industrial cable production. The results of infrared spectroscopy show that the auxiliary crosslinkers have been successfully grated to polyethylene molecules through the UV-initiation process. The space-charge characteristics achieve a significant improvement consistent with the theoretical estimation that deeper electronic traps can be introduced by auxiliary crosslinker and will consequently suppress space-charge accumulation through a trapping mechanism. Meanwhile, the conductivity of XLPE observably increases after using TMPTMA auxiliary crosslinkers at various temperatures of cable operation. The first-principles calculations also demonstrate that substantial electronic bound states have been introduced at the band edge of polyethylene molecules crosslinked by TMPTMA, leading to reduction in electrical conductivity. On the advantage of ameliorating DC dielectric performance by way of UV-initiated crosslinking process, the present research suggests a substantial strategy in XLPE cable industrial productions.
Characterizing the Complex Modulus of Asphalt Concrete Using a Scanning Laser Doppler Vibrometer
Authors: Hasheminejad Navid , Vuye Cedric , Margaritis Alexandros , Van den bergh Wim , Dirckx Joris , Vanlanduit Steve
Published: October 2019

Asphalt mixtures are the most common types of pavement material used in the world. Characterizing the mechanical behavior of these complex materials is essential in durable, cost-effective, and sustainable pavement design. One of the important properties of asphalt mixtures is the complex modulus of elasticity. This parameter can be determined using different standardized methods, which are often expensive, complex to perform, and sensitive to the experimental setup. Therefore, recently, there has been considerable interest in developing new, easier, and more comprehensive techniques to investigate the mechanical properties of asphalt. The main objective of this research is to develop an alternative method based on an optical measurement technique (laser Doppler vibrometry). To do this, a frequency domain system identification technique based on analytical formulas (Timoshenko’s beam theory) is used to determine the complex modulus of asphalt concrete at its natural frequencies and to form their master curve. The master curve plotted by this method is compared with the master curve obtained from the standard four-point bending test, and it is concluded that the proposed method is able to produce a master curve similar to the master curve of the standard method. Therefore, the proposed method has the potential to replace the standard stiffness tests. Furthermore, the standard stiffness methods usually conduct experiments up to the maximum frequency of 30 Hz. However, the proposed method can provide accurate complex modulus at high frequencies. This makes an accurate comparison between the properties of the asphalt mixtures in high frequencies and the development of more accurate theoretical models for simulation of specimens possible.
Acquisition of Dynamic Material Properties in the Electrohydraulic Forming Process Using Artificial Neural Network
Authors: Woo Min-A , Moon Young-Hoon , Song Woo-Jin , Kang Beom-Soo , Kim Jeong
Published: October 2019

Electrohydraulic forming is a high-velocity forming process that deforms sheet metals with velocities above 100 m/s and strain rates more than 100 s−1. This experiment was conducted in a closed space because of safety concerns related to the high-velocity conditions; therefore, we were not able to examine the deformation process of the sheet metal. To observe the electrohydraulic forming process in detail, we performed virtual numerical simulations using accurate material properties. Therefore, in this paper, we obtained the material property of a sheet metal from a numerical estimation by using a surrogate model based on the reduced order model and the artificial neural network. The Cowper–Symonds constitutive equation was selected for the Al 6061-T6 sheet metal, and two strain rate parameters were adopted as the unknown parameters. From the two sampling techniques, the training and test samples were extracted from the specific ranges of two unknown parameters, and a numerical simulation was performed for these samples by using the LS-DYNA program. The z-axis displacements of the deformed sheet metal were obtained from the results of the numerical simulation, and two basis vectors were extracted by using principal component analysis. In addition, to predict the weighting coefficients of the two basis vectors at the defined range of parameters, we used the artificial neural network technique as a surrogate model. By comparing the surrogate model and the experimental results and calculating the root mean square error value, we estimated the optimal parameter for Al 6061-T6. Finally, the reliability of the obtained material parameters was proved by comparing the experimental results, the surrogate model, and LS-DYNA.
Importance of Cation Species during Sulfate Resistance Tests for Alkali-Activated FA/GGBFS Blended Mortars
Authors: Cho Youngkeun , Kim Joo Hyung , Jung Sanghwa , Chung Yoonseok , Jeong Yeonung
Published: October 2019

In this study, the changes in mass, compressive strength, and length of blended mortars were analyzed to investigate their sulfate resistance according to the ground granulated blast furnace slag (GGBFS) blending ratio and type of sulfate solution applied. All alkali-activated mortars showed an excellent sulfate resistance when immersed in a sodium sulfate (Na2SO4) solution. However, when immersed in a magnesium sulfate (MgSO4) solution, different sulfate resistance results were obtained depending on the presence of GGBFS. The alkali-activated GGBFS blended mortars showed a tendency to increase in mass and length and decrease in compressive strength when immersed in a magnesium sulfate solution, whereas the alkali-activated FA mortars did not show any significant difference depending on the types of sulfate solution applied. The deterioration of alkali-activated GGBFS blended mortars in the immersion of a magnesium sulfate solution was confirmed through the decomposition of C–S–H, which is the reaction product from magnesium ions, and the formation of gypsum (CaSO4·2H2O) and brucite (Mg(OH)2).
Spark Plasma Sintering Behavior of Nb-Mo-Si Alloy Powders Fabricated by Hydrogenation-Dehydrogenation Method
Authors: Lee Sung , Park Ki , Kang Jang-Won , Kim Yanghoo , Kang Hyun-Su , Ha Tae , Min Seok-Hong , Park Hyung-Ki
Published: October 2019

In this study, the sintering behaviors of Nb-6Mo-20Si-3Cr (at percentage) in situ composite powders were studied. The Nb alloy powder was fabricated by a hydrogenation-dehydrogenation method, and both the alloy ingot and powders consisted of two phases: An Nb metal phase and the α-Nb5Si3 phase. Consolidation of the alloy powders was performed at 1500, 1600, and 1700 °C using spark plasma sintering, and the microstructures and phases formed at various sintering temperatures were analyzed. Micropores were observed in the compact sintered at 1500 °C due to the lack of complete densification at that temperature. The densification was completed at 1600 °C and the microstructure was slightly coarsened at 1700 °C compared to the microstructure of the compact sintered at 1600 °C. The microstructures prepared by the powder metallurgy method were finer than the microstructure of the ingot prepared by the casting method. The phase formation behavior varied according to the sintering temperature. Specifically, the α-Nb5Si3 phase, which is a stable structure of the Nb5Si3 phase at a low temperature, was transformed to the β-Nb5Si3 phase (which is stable at a high temperature) with an increasing sintering temperature.
Pd-Ag Electrical Resistivity in Hydrogen and Deuterium: Temperature Effect
Authors: Pozio Alfonso , Tosti Silvano
Published: October 2019

The electrical resistivity of Pd-Ag (silver 21 wt.%) in hydrogen and deuterium atmosphere at 100 kPa has been investigated via electrochemical impedance spectroscopy. The electrical resistivity of Pd-Ag vs. the temperature presents the characteristic S-shaped curve with a minimum and a maximum of the resistivity in different positions for the tests in hydrogen and deuterium. The results have been related to: (1) the different isotope ratios, H/M and D/M, and (2) their position in the Pd-Ag lattice. The behavior of the electrical resistivity is discussed in details by considering the hydrogen and deuterium uploading into the alloy, its effect on the conduction electrons, and the scattering of the isotopes atoms into the metal lattice. Measurements carried out in hydrogen with slow temperature ramping between 25–250 °C evidenced a hysteresis effect that can be explained by the different energy levels of isotopes in O-sites and T-sites
Exponentially Complex “Classically Entangled” States in Arrays of One-Dimensional Nonlinear Elastic Waveguides
Authors: Deymier P.A. , Runge K. , Hasan M. A. , Calderin L.
Published: October 2019

We demonstrate theoretically, using multiple-time-scale perturbation theory, the existence of nonseparable superpositions of elastic waves in an externally driven elastic system composed of three one-dimensional elastic wave guides coupled via nonlinear forces. The nonseparable states span a Hilbert space with exponential complexity. The amplitudes appearing in the nonseparable superposition of elastic states are complex quantities dependent on the frequency of the external driver. By tuning these complex amplitudes, we can navigate the state’s Hilbert space. This nonlinear elastic system is analogous to a two-partite two-level quantum system.
Tuning the Photophysical Features of Self-Assembling Photoactive Polypeptides for Light-Harvesting
Authors: Michalik Maciej , Zbyradowski Mateusz , Heriyanto  , Fiedor Leszek
Published: October 2019

The LH1 complex is the major light-harvesting antenna of purple photosynthetic bacteria. Its role is to capture photons, and then store them and transfer the excitation energy to the photosynthetic reaction center. The structure of LH1 is modular and it cooperatively self-assembles from the subunits composed of short transmembrane polypeptides that reversibly bind the photoactive cofactors: bacteriochlorophyll and carotenoid. LH1 assembly, the intra-complex interactions and the light-harvesting features of LH1 can be controlled in micellar media by varying the surfactant concentration and by adding carotenoid and/or a co-solvent. By exploiting this approach, we can manipulate the size of the assembly, the intensity of light absorption, and the energy and lifetime of its first excited singlet state. For instance, via the introduction of Ni-substituted bacteriochlorophyll into LH1, the lifetime of this electronic state of the antenna can be shortened by almost three orders of magnitude. On the other hand, via the exchange of carotenoid, light absorption in the visible range can be tuned. These results show how in a relatively simple self-assembling pigment-polypeptide system a sophisticated functional tuning can be achieved and thus they provide guidelines for the construction of bio-inspired photoactive nanodevices.
Use of Superelastic Nitinol and Highly-Stretchable Latex to Develop a Tongue Prosthetic Assist Device and Facilitate Swallowing for Dysphagia Patients
Authors: Shayan Mahdis , Gildener-Leapman Neil , Elsisy Moataz , Hastings Jack T. , Kwon Shinjae , Yeo Woon-Hong , Kim Jee-Hong , Shridhar Puneeth , Salazar Gabrielle , Chun Youngjae
Published: October 2019

We introduce a new tongue prosthetic assist device (TPAD), which shows the first prosthetic application for potential treatment of swallowing difficulty in dysphagia patients. The native tongue has a number of complex movements that are not feasible to mimic using a single mechanical prosthetic device. In order to overcome this challenge, our device has three key features, including (1) a superelastic nitinol structure that transfers the force produced by the jaws during chewing towards the palate, (2) angled composite tubes for guiding the nitinol strips smoothly during the motion, and (3) highly stretchable thin polymeric membrane as a covering sheet in order to secure the food and fluids on top of the TPAD for easy swallowing. A set of mechanical experiments has optimized the size and angle of the guiding tubes for the TPAD. The low-profile TPAD was successfully placed in a cadaver model and its mobility effectively provided a simplistic mimic of the native tongue elevation function by applying vertical chewing motions. This is the first demonstration of a new oral device powered by the jaw motions in order to create a bulge in the middle of the mouth mimicking native tongue behavior.
Effect of Electropulsing Treatment on Microstructure and Mechanical Properties of a Deformed ZrTiAlV Alloy
Authors: Yang Zhi Nan , Jiang Feng , Wang Xu Biao , Qu Lin , Li Yan Guo , Chai Lin Jiang , Zhang Fu Cheng
Published: October 2019

In contrast to conventional heat treatment processes, electropulsing not only heats an alloy, but also exerts some other positive effects during the heating process. In this paper, the microstructural evolution and mechanical properties of a deformed Zr40Ti5Al4V alloy after electropulsing treatment were investigated. The results showed that when the charging voltage was 2 kV, there was a slight decrease in dislocation density due to the electron wind which softened the alloy even though the highest temperature of the specimen during the treatment was only 86 °C. Increasing the charging voltage to 6 kV not only further increased the heating temperature, but accelerated the phase transformation process of α″ → β → α. The presence of the α phase strengthened the alloy but notably deteriorated its ductility. A full and refined β phase microstructure could be obtained when the charging voltage was increased to 8 kV. This simultaneously increased the strength and ductility of the alloy.
An Investigation on the Usability of Acceleration Test by Impressed Anodic Current for Evaluating Corrosion Behavior of Hot-Dip Galvanized Rebar in Concrete
Authors: Choe Hong-bok , Nishio Yuhei , Kanematsu Manabu
Published: October 2019

Hot-dip Galvanized rebar (hereafter, HDG rebar) has an anti-corrosion effect due to the sacrificial anodic reaction of zinc. Additionally, the zinc coating itself provides barrier protection for the steel substrate. Meanwhile, from one of the investigations on the field performance of HDG rebar in concrete, HDG rebar did not protect the substrate when the remaining zinc was under 50 µm. For the evaluation of this property over a short period of time, an acceleration test using impressed anodic current (hereafter, acceleration test) may be useful. This test impresses constant direct current into the rebar and can result in the intended quantitative extent of the anodic reaction. However, in using this test on HDG rebar, it was found that the high rate of applied current density could cause an unintended early end of the anti-corrosion effect of zinc, despite there being more than 50 µm of remaining zinc thickness. In this study, the usability of the acceleration test was investigated to determine if it is a suitable method for evaluating the anti-corrosion behavior of HDG rebar in concrete. As a test variable, a comparatively low rate of applied current density was used in the experiments. As a result, it was clarified that an effective corrosion protection of the substrate was made with an increase of the zinc corrosion amount. This anti-corrosion effect was similar to that known to exist in actual corrosion environments. This behavior was terminated when the concrete cracked, and the substrate became corroded. While the test condition in this study resulted in an early end of the anti-corrosion effect of zinc, a linear correlation was achieved between the applied current density and the remaining zinc thickness at the time that the anti-corrosion effect was terminated. It was found that lowering the applied current density resulted in a more suitable test condition. In conclusion, the acceleration test was found to be useful, although further experimental validation is necessary to confirm this finding.
Broadband Near-Infrared Absorber Based on All Metallic Metasurface
Authors: Zhang Ke , Deng Ruixiang , Song Lixin , Zhang Tao
Published: October 2019

Perfect broadband absorbers have increasingly been considered as important components for controllable thermal emission, energy harvesting, modulators, etc. However, perfect absorbers which can operate over a wide optical regime is still a big challenge to achieve. Here, we propose and numerically investigate a perfect broadband near-infrared absorber based on periodic array of four isosceles trapezoid prism (FITP) unit cell made of titanium (Ti) over a continuous silver film. The structure operates with low quality (Q) factor of the localized surface plasmon resonance (LSPR) because of the intrinsic high loss, which is the foundation of the broadband absorption. The high absorption of metal nanostructures mainly comes from the power loss caused by the continuous electron transition excited by the incident light inside the metal, and the resistance loss depends on the enhanced localized electric field caused by the FITP structure. Under normal incidence, the simulated absorption is over 90% in the spectrum ranging from 895 nm to 2269 nm. The absorber is polarization-independent at normal incidence, and has more than 80% high absorption persisting up to the incident angle of ~45° at TM polarization.
A Combination of Keyhole GTAW with a Trapezoidal Interlayer: A New Insight into Armour Steel Welding
Authors: Fei Zhenyu , Pan Zengxi , Cuiuri Dominic , Li Huijun , Gazder Azdiar A.
Published: October 2019

The ballistic performance of armour steel welds using austenitic filler materials is poor on account of the disparity in the mechanical properties of the weld and base metals. Consequently, a novel Keyhole Gas Tungsten Arc Welding process with a trapezoidal AISI309 austenitic stainless steel interlayer was developed to tailor chemical composition and microstructure by controlling the solidification sequence. Results show that the dilution rate in the weld metal region can reach up to 43.5% by placing a specially designed interlayer in between the base metal, providing a major scope for microstructure modification. Detailed weld analysis was undertaken by X-ray diffraction, optical and secondary and transmission electron microscopy, energy dispersive spectroscopy and electron back-scattering diffraction. The results from Vickers hardness indents and Charpy impact toughness testing at −40 °C show that the properties of the weld metal region are comparable to that of the base metal. This is ascribed to the weld metal comprising a two phase microstructure of martensite and retained austenite, which contribute to improvements in strength and toughness, respectively. Furthermore, the tailored chemical composition, microstructure and low temperature phase transformation in the weld metal may reduce the tendency toward both solidification cracking and hydrogen assisted cold cracking.
Robust Memristor Networks for Neuromorphic Computation Applications
Authors: Hajtó Dániel , Rák Ádám , Cserey György
Published: October 2019

One of the main obstacles for memristors to become commonly used in electrical engineering and in the field of artificial intelligence is the unreliability of physical implementations. A non-uniform range of resistance, low mass-production yield and high fault probability during operation are disadvantages of the current memristor technologies. In this article, the authors offer a solution for these problems with a circuit design, which consists of many memristors with a high operational variance that can form a more robust single memristor. The proposition is confirmed by physical device measurements, by gaining similar results as in previous simulations. These results can lead to more stable devices, which are a necessity for neuromorphic computation, artificial intelligence and neural network applications.
Viscoplastic Couette Flow in the Presence of Wall Slip with Non-Zero Slip Yield Stress
Authors: Damianou Yiolanda , Panaseti Pandelitsa , Georgiou Georgios C.
Published: October 2019

The steady-state Couette flow of a yield-stress material obeying the Bingham-plastic constitutive equation is analyzed assuming that slip occurs when the wall shear stress exceeds a threshold value, the slip (or sliding) yield stress. The case of Navier slip (zero slip yield stress) is studied first in order to facilitate the analysis and the discussion of the results. The different flow regimes that arise depending on the relative values of the yield stress and the slip yield stress are identified and the various critical angular velocities defining those regimes are determined. Analytical solutions for all the regimes are presented and the implications for this important rheometric flow are discussed.
In Vitro Simulation and In Vivo Assessment of Tooth Wear: A Meta-Analysis of In Vitro and Clinical Research
Authors: Koletsi Despina , Iliadi Anna , Eliades Theodore , Eliades George
Published: October 2019

Tooth wear may be described as a side-effect of occlusal forces that may be further induced by the common use of contemporary prosthetic materials in practice. The purpose of this systematic review was to appraise existing evidence on enamel wear from both in vitro and clinical research and explore whether evidence from these study designs lies on the same direction. Five databases of published and unpublished research were searched without limitations in August 2019 and study selection criteria included in vitro and clinical research on enamel tooth wear. Study selection, data extraction, and risk of bias assessment were done independently and in duplicate. Random effects meta-analyses of standardized mean differences (SMDs) or weighted mean differences (WMDs) with 95% confidence intervals (CIs) were conducted while a Monte Carlo permutation test for meta-regression on the exploration of the effect of the study design on the reported outcomes was planned. A total of 27 studies (23 in vitro and 4 clinical) were eligible while 12 contributed to meta-analyses. Overall, some concerns were raised for the quality of the existing evidence and the potential for risk of bias. Enamel wear (mm) of antagonist teeth was more pronounced when opposed to conventional porcelain compared to machinable ceramics (SMD = 2.18; 95%CIs: 1.34, 3.02; p < 0.001). Polished zirconia resulted in decreased volumetric enamel wear (mm3) of opposing teeth compared to pure natural enamel (SMD = –1.06; 95%CIs: –1.73, –0.39; p = 0.002). Monolithic zirconia showed evidence of enhanced potential for antagonist wear (μm) compared to natural teeth (WMD = 107.38; 95%CIs: 30.46, 184.30; p = 0.01). Study design did not reveal an effect on the tooth wear outcome for the latter comparison when both clinical and in vitro studies were considered (three studies; Monte Carlo test, p = 0.66). In conclusion, there is an overriding need for additional evidence from clinical research to substantiate the findings from the already existing laboratory simulation studies.
Evaluation of Targeted Delivery to the Brain Using Magnetic Immunoliposomes and Magnetic Force
Authors: Thomsen Louiza Bohn , Linemann Thomas , Birkelund Svend , Tarp Gitte Abildgaard , Moos Torben
Published: October 2019

Magnetic nanoparticles have great prospects for drug delivery purposes, as they can be designed with various surface coatings and conjugated with drugs and targeting moieties. They also have a unique potential for precise delivery when guided by magnetic force. The blood-brain barrier (BBB) denotes the interface between the blood and brain parenchyma and hinders the majority of drugs from entering the brain. Red fluorescent magnetic nanoparticles were encapsulated in liposomes and conjugated to antibodies targeting the rat transferrin receptor (OX26) to form magnetic immunoliposomes. These magnetic immunoliposomes enhanced the uptake by rat brain capillary endothelial cells (BCECs) in vitro. In situ brain perfusion in young rats high in the endogenous expression of transferrin receptors by BCECs, revealed enhanced uptake of magnetic immunoliposomes when compared to naked magnetic nanoparticles or non-targeted magnetic liposomes. When applying the external magnetic force, the magnetic nanoparticles were detected in the brain parenchyma, suggesting transport across the BBB. Ultrastructural examination of the immunoliposomes, unfortunately, was unable to confirm a complete encapsulation of all naked nanoparticles within the liposomes, suggesting that the data on the brain could derive from particles being released from the liposomes under influence of external magnetic force; hence hypothesizes on external magnetic force as a qualifier for dragging targeted magnetic immunoliposomes through the BBB. In conclusion, our results suggest that transport of magnetic nanoparticles present in BCECs by targeted delivery to the transferrin receptor may undergo further transport into the brain when applying magnetic force. While magnetic immunoliposomes are targetable to BCECs, their design to enable further transport across the BBB when applying external magnetic force needs further improvement.
Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells
Authors: Doubkova Martina , Nemcakova Ivana , Jirka Ivan , Brezina Vitezslav , Bacakova Lucie
Published: October 2019

Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH2O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants.
Fabrication of Metal Matrix Composite by Laser Metal Deposition—A New Process Approach by Direct Dry Injection of Nanopowders
Authors: Lanfant Briac , Bär Florian , Mohanta Antaryami , Leparoux Marc
Published: October 2019

Laser Metal Deposition (LMD) offers new perspectives for the fabrication of metal matrix nanocomposites (MMnCs). Current methods to produce MMnCs by LMD systematically involve the premixing of the nanopowders and the micropowders or require in-situ strategies, thereby restricting the possibilities to adjust the nature, content and location of the nano-reinforcement during printing. The objective of this study is to overcome such restrictions and propose a new process approach by direct injection of nanoparticles into a metallic matrix. Alumina (n-Al2O3) nanoparticles were introduced into a titanium matrix by using two different direct dry injection modes in order to locally increase the hardness. Energy dispersive X-ray spectroscopy (EDS) analyses validate the successful incorporation of the n-Al2O3 at chosen locations. Optical and high resolution transmission electron microscopic (HR-TEM) observations as well as X-ray diffraction (XRD) analyses indicate that n-Al2O3 powders are partly or totally dissolved into the Ti melted pool leading to the in-situ formation of a composite consisting of fine α2 lamellar microstructure within a Ti matrix and a solid solution with oxygen. Mechanical tests show a significant increase in hardness with the increase of injected n-Al2O3 amount. A maximum of 620 HV was measured that is almost 4 times higher than the pure LMD-printed Ti structure.
Recyclable Polymer-Supported N-Hydroxyphthalimide Catalysts for Selective Oxidation of Pullulan
Authors: Culica Madalina Elena , Kasperczyk Kornela , Baron Raluca Ioana , Biliuta Gabriela , Macsim Ana Maria , Lazea-Stoyanova Andrada , Orlinska Beata , Coseri Sergiu
Published: October 2019

This paper proposes a convenient route to oxidize the –CH2–OH groups in the water-soluble pullulan, using a new catalytic polymer-supported N-hydroxyphthalimide (NHPI) immobilized on polystyrene. The protocol involves the presence of sodium hypochlorite and sodium bromide. The conversion is possible at room temperature, atmospheric pressure, and pH = 10. The characterization of both the catalysts and oxidized pullulan was done using NMR and FTIR methods. Using polyelectrolyte titration with end-point indication by means of a particle-charge detector (PCD), we were able to assess the degree of electrokinetic charge in all oxidized samples as a consequence of the conversion of the –CH2–OH group into –COOH moieties. The possibility of recovery and recycling of the polymer-supported NHPI catalyst was tested for up to four cycles, since the morphological analyses performed on the catalysts using SEM revealed no significant changes.