bluuebunny/crossref_metadata_2025 huggingface dataset:
PENERAPAN MODEL PEMBELAJARAN THE POWER OF TWO UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS V SD NEGERI 56 KOTA TERNATE PADA TEMA 6 SUBTEMA 2 PERPINDAHAN KALOR DI SEKITAR KITA
Authors: Agus Neli Yusliana , Karim Kodrat Hi , Purnomo Eko
Published: January 2024

The purpose of this study is to express the learning model of The Power Of Two in grade V students of SD Negeri 56 Ternate City, after using the learning model The Power Of Two in Theme 6 Subtheme 2 Heat Transfer Around Us. This research is Classroom Action Research (CAR). The subjects of the study were class V students with a total of 26 students consisting of 9 men and 17 women. Data collection techniques in this study are observation, documentation and tests. Data analysis techniques in this study were carried out in three stages, namely to determine individual student learning outcomes, to determine the completeness of student learning classically and to calculate the percentage of student and teacher activity results.The results of this study were 2 cycles, where cycle I obtained learning completeness with a percentage of 42.30% as many as 11 students, student activity 65.27% and teacher activity 68.75%. Then in the second cycle, student learning completeness was obtained with 96.15% as many as 25 students, student activity 90.27% and teacher activity 93.75%. Based on the results of the percentage above, it can be concluded that Learning Using The Power Of Two Model In Learning Theme 6 Subtheme 2 Heat Transfer Around Us can improve the learning outcomes of grade V students of SD Negeri 56 Ternate City.
PENGEMBANGAN VIDEO ANIMASI BERBASIS POWTOON PADA MATERI EKOSISTEM SISWA KELAS IV SD NEGERI 56 KOTA TERNATE
Authors: Basahona Anriyani A , Pamuti Pamuti , Nadra Wawan S
Published: January 2024

The aims of this study were (1) to determine the development of Powtoon-based animated video media on ecosystem material for fifth grade students of SD Negeri 54 Ternate City to meet valid criteria (2) to determine the development of Powtoon-based animated video media on ecosystem material for fifth grade elementary school students Negeri 54 Kota Ternate can meet practical criteria (3) to find out the effectiveness of developing Powtoon-based animated video media on ecosystem material to improve learning outcomes for class V students at SD Negeri 54 Kota Ternate.This research is research and development (RD) using the Borg and Gall development model modified by Sugiyono (2007) consisting of ten steps and this research is limited to the sixth step namely potentials and problems, data collection, product design, design validation, design revision , and product trials. The validators who validated the development of Powtoon-based animated video media were media experts, science subject matter experts, media practicality experts by teachers and student responses. This data collection technique is through a feasibility validation instrument for Powtoon-based animated video media on ecosystem material using interviews, questionnaires, test questions, and documentation. The data analysis technique uses a quantitative descriptive technique. The research results show that; The development of Powtoon-based animated video media on ecosystem materials obtained an average score of 93% from both expert lecturers and the media was declared very valid, feasible to use; animated video media based on Powtoon on ecosystem material is stated to be very practical and feasible to use in the learning process which gets an average score of 87%, this value is obtained from a teacher's questionnaire and student responses; Powtoon-based animated video media on ecosystem material was declared effective which was obtained from the average posttest score of 89 from KKM ≥70 and from a total of 18 students. With this it can be concluded that the development of Powtoon-based animated video media on ecosystem material for Grade V Students of SD Negeri 54 Ternate City can be stated as media that meets the criteria of being valid, practical, and effective for use in the learning process by teachers and students.
PENGGUNAAN MEDIA KOMBINASI (FIELD TRIP DAN DEMONSTRASI) UNTUK MENINGKATKAN HASIL BELAJAR PESERTA DIDIK MATERI PEMANFAATAN SUMBER DAYA ALAM MATA PELAJARAN IPA KELAS IV SD MUHAMMADIYAH 2 KOTA MALANG
Authors: Abdy Iwan , Nurharyanto Dwi W , Susilawati L
Published: January 2024

Media pembelajaran dalam proses belajar mengajar dapat digunakan untuk mencapai hasil yangmaksimal. Diharapkan guru memberikan pengajaran yang menarik dan mudah dipahami serta menumbuhkan suasana belajar yang menyenangkan sehingga peserta didik tidak bosan. 23 peserta didik SD Muhammadiyah 2 kelas IV berpartisipasi dalam penelitian ini. metode Field Trip dapat dimanfaatkan dalam pendidikan IPA dengan mengajak peserta didik belajar di luar kelas seperti ke perkebunan atau museum. Field Trip adalah upaya guru untuk memberikan pengalaman secara otentik bagi peseta didik untuk meningkatkan keterampilan sosial, menyalurkan pengetahuan peserta didik, mendorong peserta didik agar berpikir kreatif, serta melihat dan mengetahui masalah secara langsung. Sedangkan demonstrasi merupakan suatu teknik penyajian pengajaran dengan memperlihatkan serta menampilkan suatu objek kepada peserta didik. Prasiklus, Siklus I, dan Siklus II merupakan tiga tahapan penelitian. Pada Siklus I, menggunakan media visual Field Trip yang membantu 19 peserta didik (83%) memperoleh hasil belajar di atas KKM. Perbaikan pembelajaran Siklus II dilakukan bertujuan untuk lebih memantapkan pemahaman peserta didik dengan menggunakan metode demonstrasi. Pada penelitian Siklus II hasil belajar diatas KKM sebanyak 21 peserta didik (91%) dengan nilai tertinggi 100 dan terendahnya 65.
EMERGENCE, DEVELOPMENT AND SUSTAINABILITY OF INNOVATIVE ENTERPRISES
Authors: MIŠÚNOVÁ HUDÁKOVÁ IVANA , SLÁVIK ŠTEFAN , MIŠÚN JURAJ
Published: January 2024

Currently, innovations introduced in companies that are exposed to a complex business environment are a hot topic of discussion. Their growth and sustainability are intrinsically linked to innovation processes. In order for enterprises to move forward, this very issue with its emphasis on strategic sustainability in such enterprises represents, in our view, a research gap that needs to be continuously explored in greater depth. In the present paper, we focus on Slovak enterprises that are based on the development of new products in Slovakia and which are also part of a stagnant innovation environment. Attention is drawn to selected aspects influencing innovation in enterprises, which should be perceived by companies not as a burden, but as an intrinsic need for their further development and their sustainability in the long term.
KORELASI PENDEKATAN EKOLOGI DENGAN KETERAMPILAN MENULIS PUISI SISWA KELAS VIII SMP NEGERI 3 KAYUAGUNG
Authors: Purnamalia Triska , Kencana Agnes Tri
Published: January 2024

Data collection techniques were carried out through written tests, and the answers to questions were verified through content validity. Data analysis techniques make the initial score frequency distribution, classify values, determine the correlation using the product moment correlation formula, calculate the reliability of the test and provide an interpretation of the correlation coefficient obtained. The results of this study indicate a correlation between the variables X and Y. This is evidenced by the results of testing the rxy number of 0.567 and the product moment correlation table of 0.463. Comparing these values means that the r-number is greater than the r-table (0.567 > 0.463). The values obtained show a significant relationship in that the r-number is greater than the r-table. From the results of poetry analysis and observations at school, writing poetry is related to ecology, because writing poetry requires inspiration from the surrounding environment and the most important elements that occur in the environment when creating beautiful and interesting literary works
PENGARUH MEDIA BALOK HURUF TERHADAP KETERAMPILAN MEMBACA PADA SISWA TUNAGRAHITA KELAS II SLB NEGERI PURWOSARI
Authors: Ermawati Diana , Wati Mesyiana Cintiya , Destantya Sinta , Sari Ninis Fivtia
Published: January 2024

The objective of the research is to identify disparities in the early reading proficiency of mentally challenged pupils when it comes to letter recognition using letter block media. The ultimate goal is to enhance their reading ability through targeted interventions. The employed approach is a quantitative experimental methodology, involving the gathering of data through observation, interviews, testing, and documentation on students with intellectual disabilities in class II SLB Negeri Purwosari. The data analysis technique employs a paired sample t-test. The research findings indicate that the utilization of letter block media is substantial, as demonstrated by the pretest mean score of 44.62 and the posttest mean score of 76.46. Pre- and post-application data analysis shown a notable enhancement in alphabet letter recognition and early reading skills among class II mentally challenged students at Purwosari State Special School. This conclusion can be drawn by comparing the Ttable value with the Tcount value, where the Tcount value is 0.003 and the Ttable value is 0.05, while employing letter block media
RING1 Inhibition Has a Cell-Specific Antitumoral Role by Promoting Autophagy in Endometrial Cancer Cells
Authors: Krześlak Anna , Szustka Aleksandra , Kozal Karolina
Published: January 2024

Background/Aims: Factors influencing gene expression through chemical modifications of histones may play an important role in the regulation of the autophagy process in cancers. RING1A or RING1B are responsible for the catalytical activity of Polycomb repressive complex 1 (PRC1) which monoubiquitylate histone H2A. The aim of the study was to determine the effect of the RING1A/B protein inhibition on the autophagy process in endometrial cancer cells and the anticancer effectiveness of RING1 inhibitor PRT4165 in combination with autophagy inhibitors. Methods: The expression of autophagy genes and proteins were analyzed in endometrial cancer cells HEC-1A and Ishikawa grown in different glucose concentrations and treated with PRT4165. To assess the effectiveness of PRT4165 used alone or in combination with HCQ or Lys05, IC50 and the combination index (CI) were calculated. Flow cytometry method was used to estimate apoptotic cells after treatment. Results: The results confirm the impact of RINGs on autophagy and apoptosis in endometrial cancer cells. PRT4165 inhibitor causes changes in the expression of ATG genes and autophagy markers and the effect depends on glucose concentration and cell types. However, the anticancer effectiveness of PRT4165 was lower when it was used in combination with autophagy inhibitors, suggesting that such a combination is not a promising anticancer strategy. Conclusion: The results indicate the importance of the RINGs in the process of autophagy and apoptosis. Further potentially more effective combinations of PRT4165 with autophagy modulators should be sought.
A Study on Preconceptions of Elementary School Students Regarding Aviation Safety
Authors:   The Korean Society of Culture and Convergence, Na SoJung
Published: January 2024

This study aims to understand preconceptions about aviation safety and explore foundational data for developing age-specific transportation safety, particularly aviation safety, education courses and content throughout the lifespan. Targeting elementary school 3rd-grade students, who initiate basic logical reasoning, the research employs semi-structured questions, descriptive interviews, and drawing techniques to assess preconceptions about aviation safety in the early stages of childhood. The findings reveal six types of preconception awareness: causal factors of accidents, forms of accidents, safety rules, risk and safety factors, awareness of seat safety, and visual perception of airplane safety. In conclusion, the study proposes the utilization of these preconceptions through a comparative analysis with scientific concepts in actual aviation safety education. This information can inform the development of educational content and instructional designs for aviation safety in the future, tailored to the developmental stages of students.
The Functions of Soundscapes in René Magritte's Works
Authors:   The Korean Society of Culture and Convergence, Noh Shihun
Published: January 2024

The purpose of this study is to specifically analyze the function of the soundscapes formed by the auditory images latent in René Magritte's works and examine their meaning. As a result of analyzing the soundscape elements in his 1,957 works, it was found that ‘instrument’, ‘score’, and ‘bell’ form theme groups, and ‘tuba’, ‘score’, and ‘bell’ represent each subject group. The ‘score’ included in the work through collage functions to evoke auditory images in the art work in silence, breaking the hierarchy of senses absolutely dominated by sight and the discord between sense organs. The ‘tuba’, juxtaposed with Magritte’s icons in an unexpected way, functions to give a subversive poetic meaning to reality by evoking shock and mystery in the work. In the work, ‘bell’ has the function of making us aware of the everyday existence of soundscapes and revealing the mystery behind them. The soundscapes that serve various functions in Magritte's works are meaningful in that they demonstrate the possibility of a new expressive method of surrealism as a whole.
Development of Cultural Heritage Play Diocese to Improve User Concentration
Authors:   The Korean Society of Culture and Convergence, Yoon Easil , Lee Jinwook
Published: January 2024

Through a parish development support project called “Unusual Research Laboratory”, a teaching tool for culture and arts education related to local cultural heritage linked to the social content of elementary school was developed in the form of a board game. It aims to improve the concentration of cultural heritage assets by utilizing board game play teaching tools from a geographical and historical perspective for elementary school students living in Gwangju Metropolitan City. Qualitative and quantitative evaluations of the experimental group and interviews of expert groups were conducted for the effectiveness of the thesis. As a result, it was verified that education on cultural heritage, which can be less concentrated, has increased the time, efficiency, and interest of concentration through the board game play teaching tool. Through this study, it is hoped that follow-up researchers and related teaching tool industries will develop and study play teaching tools from various perspectives to improve user concentration.
An Interview-based Investigation into Bilingual Revision Practice
Authors:   The Korean Society of Culture and Convergence, Kim Jagyeong
Published: January 2024

This study aims to investigate bilingual revision practice by conducting interviews with five bilingual revisers and one project manager. The participants were asked about the reading order between the source and the target text during the revision process, the possibility of a reduction in revision stages, and revision parameters and scope. The findings showed that more participants started revision by reading the target text but those with stronger focus on the correction of accuracy errors read the source text first; a reduction of revision stages looks possible depending on time and budget constraints and client needs; bilingual revisers stressed the importance of correcting accuracy errors but language and presentation were also considered important, while content checking was mostly considered the task of translators, especially from the perspective of freelance revisers; and the scope of revision seemed to be strongly influenced by the needs and expectations of clients. This study sheds some light on real-life revision practice, showing the need of further investigation to better understand the complicated revision practice.
Analysis of Sound Montage in Video Game Cut Scenes
Authors:   The Korean Society of Culture and Convergence, Park Sojeong , Han Kyunghoon
Published: January 2024

The purpose of this study is to analyze and derive the roles of music and sound in video games using the theory of sound montage, a predominant technique in the creation of audiovisual media. The methodology involves examining selected game cut scenes by applying the theory of sound montage to illuminate the relationship between auditory and visual elements. The analysis revealed that, similar to most audiovisual media, games tightly intertwine visual scenarios with the utilization of sound, confirming the application of sound montage theory in gaming, a technique previously observed mainly in films. This signifies the active use of sound montage as a crucial directing technique to encapsulate the director's intentions within game sound. It is anticipated that this study will serve as a meaningful contribution to the academic field by filling the gap in research concerning game sound production techniques
A Direction of Use of Clay as an Multi-Sensory Arts Educational Medium to Improve Children's Self-Understanding: Focused on R. Steiner's Color Education
Authors:   The Korean Society of Culture and Convergence, Lee Nayoung , Park Sohyoung , Choi Huyncheol
Published: January 2024

The purpose of this study is to propose and outline a multisensory-integrated clay-based art education program aimed at enhancing self-concept abilities in children at the pre-schematic stage(ages 4-7). The study involves an analysis of Rudolf Steiner's educational color theory for holistic education, the Waldorf education curriculum applying his theory, and prior theories and literature on the theory of multi-sensory practical education in Waldorf. The research resulted in the clay is effective in self-awareness and understanding of children and can be used as a multisensory medium in color education. The educational program, focusing on the color, materiality, and texture of clay, is found to be effective for children to recognize themselves as part of nature and to improve their understanding of the human foundation, namely, themselves.
Designing a Mixed Writing Class: Focused on the Jeju National University’s Hi-Edu Model
Authors:   The Korean Society of Culture and Convergence, Heo Wonyoung , Jo Yuyoung
Published: January 2024

The purpose of this study is to design a Mixed Writing Class by applying Hi-Edu Model. The Hi-Edu Model is a new teaching model presented by Jeju National University, and was developed with the goal of mixing several teaching methods and teaching environments. Details of the Hi-Edu Model include two teaching methods: Problem-Based Learning(Hi-Edu 1), Flipped Learning(Hi-Edu 2), and three teaching environments: Hybrid Learning(Hi-Edu 3), Blended Learning(Hi-Edu 4), Distance Learning(Hi-Edu 5). Also depending on the mixing method, the Hi-Edu Model is classified into the first type based on Hybrid Learning(Hi-Edu 3 + (Hi-Edu 1 + Hi-Edu 2)), the second type based on Blended Learning(Hi-Edu 4 + (Hi-Edu 1 + Hi-Edu 2)), and the third type based on Distance Learning(Hi-Edu 5 + (Hi-Edu 1 + Hi-Edu 2)). When applying these classified types to the situation, Mixed Class can be designed. In particular, this study designed a Mixed Writing Class by applying the Hi-Edu Model, and looked at its features in detail.
A Study on the B-class Culture Analysis of Korean Popular Music
Authors:   The Korean Society of Culture and Convergence, Cho SungHai
Published: January 2024

The purpose of this paper is to study B-class culture in Korean popular music. B-class identity as a subculture was examined, observing the changes in B-class music since the 2000s, and cultural diversity was discussed. Following the literature review, Psy, Norazo, and UV were selected as research subjects. The external items of capital, popularity, and intentionality and the internal items of language, content, format, behavior, and image characteristics were analyzed deductively through NVivo 14. As a result, Psy's music carried B-class features in terms of language, behavior, and image, and Norazo and UV's music had them in all internal aspects. Those meanings have all been transferred to a reconceptualized B-class. They were subordinated to large capital or the mainstream system, and the consumer base expanded from the minority to the majority. Moreover, resistance has weakened and was replaced by interest. The B-class mainstreaming passed through a latent and transitional period in the 2000s and settled on one side of the popular culture in the 2010s, facing the authenticity issue. Opinions on diversifying music culture were presented, with the claims for recovery of the rights of musical cultural subjects and resolution of the imbalance in music culture.
Validation of the Korean Version of the Multidimensional State Boredom Scale
Authors:   The Korean Society of Culture and Convergence, Han Boram , Song Wonyoung
Published: January 2024

This study aimed to validate the Multidimensional State Boredom Scale (MSBS) developed by Fahlman et al. (2013). A revised preliminary scale, based on input from the original author Eastwood, was used with 146 college students. Exploratory factor analysis revealed 29 items distributed across four factors. Confirmatory factor analysis was conducted on both the four-factor model and the original author's five-factor model, using data from 279 university students. The five-factor 29-item scale was deemed more appropriate. Internal consistency for reliability was high at .966, and test-retest reliability(n=45) at 3-4 week intervals was .61. Validity was assessed through correlations between state boredom and boredom proneness, depression, and impulsivity. Significant correlations were found, supporting both criterion and convergent validity. This study will contribute to the validation of a boredom measure that will be relevant to clinical difficulties and can be utilized in the prevention and treatment of psychological problems.
The Effect of Test Anxiety on Depression and Quality of Life in Nursing Students
Authors:   The Korean Society of Culture and Convergence, Han Jeongwon
Published: January 2024

The purpose of this study is to determine the impact of test anxiety on depression and quality of life in adopted of nursing college students. The 160 copies were analyzed by conducting a survey on test anxiety, depression, and quality of life for nursing students at K university. Correlation analysis and stepwise regression analysis were conducted to confirm. First, test anxiety and depression have a positive correlation, and test anxiety and quality of life have a negative correlation. Second, the factors affecting the depression and quality of life of nursing students are emotional factors, physical mental health, mental health, and major satisfaction, which are sub-factors of test anxiety. The significance of this study is to provide basic data for mental health promotion and educational intervention preparation to reduce depressive symptoms and improve positive mental health and quality of life in nursing students in the future.
The Relationship between Self-management and Learning Flow in High School Students: Focusing on Mediating Effect of Career Maturity
Authors:   The Korean Society of Culture and Convergence, Kim Sujeong
Published: January 2024

This study examined the relationship between self-management competence and learning flow among high school students and verified the mediating effect of career maturity. This study was conducted by surveying 174 students in the first and second grades of B High School located in A City, Gyeonggi-do, and analyzing the data collected using SPSS 29.0 program. The results showed that the correlations between high school students' self-management competence, learning flow, and career maturity were all significant, and the partial mediation effect of career maturity was verified. this study suggested to foster self-management competency, a core competency of the 2022 revised curriculum.
A Study on the Meaning of Ffire Play as a Local Cultural Resource and the Perception of Local Residents: Case of Seonyujulbul-nori in Hahoe Village
Authors:   The Korean Society of Culture and Convergence, Kim Yongnam
Published: January 2024

The purpose of this study is to examine how Seonyujulbul-nori in Hahoe village is perceived by local residents as a local cultural resource. The research method used was a qualitative research method targeting local residents who had experience watching the Seonyujulbul-nori in Hahoe village. As a result of the study, it was found that the classes that enjoy culture are also becoming more diverse. Second, the play formed based on history could be connected to the interest in traditional culture. Third, it can restore the values of the region and community and create a place where local residents can empathize. Fourth, it was found that the form of the space that contained the memory was maintained and that the locality of culture was created there through the memory of history. Lastly, it was found that through continuous use, it can be used as a resource for creating an environment in harmony with local residents and for local identity. Based on the results of this study, we hope to be able to study a virtuous cycle model that preserves and utilizes local cultural resources from the perspective of sustainability in the future.
A Study on the Design Project Class Model Using the Design Thinking Process
Authors:   The Korean Society of Culture and Convergence, Song Eunyoung
Published: January 2024

In order to lay the theoretical foundation for a systematic education program on the social responsibility and role of design, this study developed a teaching model that applied a project-based design thinking process for solving human-centered social problems to the “Design and Ethics” course, which is a basic major class in the Department of Design at a four-year university in Gyeonggi-do. The project teaching method consisted of five stages: frame a question, gather inspiration, generate ideas, prototype, test to learn, and improvement of the design thinking process. The results of the class showed positive learning satisfaction in terms of learning comprehension, creative thinking, and problem-solving ability, and it was an opportunity to experience a human-centered problem-solving process. The systematic curriculum on the social responsibility and role of design was organized to effectively utilize specialized technology as a means for solving human social problems, and it is significant in that it met the demand for education of the times, which includes sharing various perspectives through team project classes and cultivating a new creative problemsolving skills. In the future, it is hoped that it will develop into a practical social design education program by linking it with multidisciplinary convergence, government agencies, private companies, and social enterprises.
ANALISIS KELAYAKAN FINANSIAL RENCANA PENGEMBANGAN USAHA TANAMAN TALAS PRATAMA PADA KEBUN PERCOBAAN UPTD BPP BOJONGGAMBIR
Authors: Idris Fatwa Nafs Al Rahman Maulana , Nurunisa Venty Fitriany
Published: January 2024

Talas yang bisa dijadikan sebagai alternatif pangan sudah mulai dibudidayakan secara masif oleh masyarakat Kabupaten Tasikmalaya. Pengembangan suatu usaha pertanian di Kabupaten Tasikmalaya maupun di wilayah lainnya seharusnya dilakukan melalui analisis kesesuaian lahan, kemampuan lahan, dan kelayakan agribisnis. Hal ini penting untuk dilakukan supaya hasil dari usaha tersebut sesuai dengan yang diharapkan. Analisis kelayakan finansial akan dilakukan melalui perhitungan analisis BEP, NPV, NET B/C ratio, dan IRR. Hasil analisis kelayakan finansial rencana pengembangan usaha tanaman talas pratama, yaitu nilai BEP produksi sebesar 1.361,44 kg, BEP harga sebesar Rp. 2.041,90, NPV 15% sebesar Rp. 72.198.502, NET B/C 15% sebesar 6,71, dan IRR sebesar 374,95%. Jadi dari analisis kelayakan finansial rencana pengembangan usaha tanaman talas pratama di kebun percobaan UPTD BPP Bojonggambir layak untuk dilakukan pengembangan usaha. Karena melalui perhitungan analisis kelayakan finansial.
SURFACE MODIFICATION OF ALUMINA-SILICA BY ADDITIVE AGENT USING SOL-GEL METHOD AS FILLER DENTAL COMPOSITE
Authors: Faza Yanwar , Takarini Veni , Febrida Renny , Karlina Elin
Published: January 2024

Background: Macro-sized and inhomogeneous distribution of ceramic filer particles make it difficult to obtain smooth surfaces of dental composite after polishing. Objective. This study aims to synthesize alumina silica (Al2O3-SiO2) fillers using sol gel method with the additives agent chitosan 5%, 10% and polyethylene glycol (PEG) 5% to produce micro particle size and evenly distributed particle as filler dental composite. Methods. The type of research is descriptive explorative. The sol-gel process was utilized to synthesize filler particles of Al2O3-SiO2. TEOS was dissolved in ethanol and mixed with acetic acid as catalyts. Al2(NO3)3 was added into the solution and mixed homogenously. Subsequently, the additive agents (Chitosan 5%, chitosan 10% and PEG) were mixed into the mixture for 30 minutes. Drying the samples for 48 hours at 60°C in the oven. The Dynamic light scattering (DLS) SZ-1 was used to evaluate the size, particle distribution and zeta potential. Result. The results showed that the addition of chitosan 10% produced a smaller size of Al2O3-SiO2 compared to other samples. A homogeneous particle distribution is shown in the sample with PEG 5%. Meanwhile, zeta potential values of the filler particles Al2O3-SiO2 with the addition of chitosan 10% shows the biggest value. Conclusion: The additive agent of chitosan 10% can modify the surface of filler Al2O3-SiO2 in order to inhibit particle growth more effectively but better particle distribution is shown in samples with PEG 5% due to the lower viscosity than chitosan thus it is easily homogenized in the solution
High-level expression of leghemoglobin in Kluyveromyces marxianus by remodeling the heme metabolism pathway
Authors: Tian Tian , Wu Xinwei , Wu Pingping , Lu Xinyi , Wang Qi , Lin Yifan , Liu Canjie , Zhou Jungang , Yu Yao , Lu Hong
Published: January 2024

Soy leghemoglobin, when bound to heme, imparts a meat-like color and flavor and can serve as a substitute for animal-derived proteins. Enhancing cellular heme synthesis improves the recombinant expression of leghemoglobin in yeast. To achieve high-level expression of leghemoglobin A (LBA) in Kluyveromyces marxianus, a food-safe yeast, large-scale heme synthesis modules were transferred into K. marxianus using yeast artificial chromosomes (KmYACs). These modules contained up to 8 native and heterologous genes to promote the supply of heme precursors and downstream synthesis. Next, eight genes inhibiting heme or LBA synthesis were individually or combinatorially deleted, with the lsc1Δssn3Δ mutant yielding the best results. Subsequently, heme synthesis modules were combined with the lsc1Δssn3Δ mutant. In the resulting strains, the module genes were all actively expressed. Among these module genes, heterologous S. cerevisiae genes in the downstream heme synthesis pathway significantly enhanced the expression of their counterparts in K. marxianus, resulting in high heme content and LBA yield. After optimizing the medium recipe by adjusting the concentrations of glucose, glycine, and FeSO4·7H2O, a heme content of 66.32 mg/L and an intracellular LBA titer of 7.27 g/L were achieved in the engineered strain in a 5 L fermentor. This represents the highest intracellular expression of leghemoglobin in microorganisms to date. The leghemoglobin produced by K. marxianus can be utilized as a safe ingredient for plant-based protein products.
Synergistic antibacterial mechanism of silver-copper bimetallic nanoparticles
Authors: Hao Zhaonan , Wang Mingbo , Cheng Lin , Si Minmin , Feng Zezhou , Feng Zhiyuan
Published: January 2024

The excessive use of antibiotics in clinical settings has resulted in the rapid expansion, evolution, and development of bacterial and microorganism resistance. It causes a significant challenge to the medical community. Therefore, it is important to develop new antibacterial materials that could replace traditional antibiotics. With the advancements in nanotechnology, it has become evident that metallic and metal oxide nanoparticles (MeO NPs) exhibit stronger antibacterial properties than their bulk and micron-sized counterparts. The antibacterial properties of silver nanoparticles (Ag NPs) and copper nanoparticles (Cu NPs) have been extensively studied, including the release of metal ions, oxidative stress responses, damages to cell integrity, and immunostimulatory effects. However, it is crucial to consider the potential cytotoxicity and genotoxicity of Ag NPs and Cu NPs. Numerous experimental studies have demonstrated that bimetallic nanoparticles (BNPs) composed of Ag NPs and Cu NPs exhibit strong antibacterial effects while maintaining low cytotoxicity. Bimetallic nanoparticles offer an effective means to mitigate the genotoxicity associated with individual nanoparticles while considerably enhancing their antibacterial efficacy. In this paper, we presented on various synthesis methods for Ag-Cu NPs, emphasizing their synergistic effects, processes of reactive oxygen species (ROS) generation, photocatalytic properties, antibacterial mechanisms, and the factors influencing their performance. These materials have the potential to enhance efficacy, reduce toxicity, and find broader applications in combating antibiotic resistance while promoting public health.
Adapting to climate change: snow load assessment of snow galleries on the Iron Ore Line in Northern Sweden
Authors: Saback Vanessa , Gonzalez-Libreros Jaime , Daescu Cosmin , Popescu Cosmin , Garmabaki A. H. S. , Sas Gabriel
Published: January 2024

The snow galleries along the Iron Ore railway line in northern Sweden have faced problems in recent years due to increasingly large snow loads, and several galleries have been damaged. These incidents motivated an evaluation of the maximum load supported by the galleries before collapse, which is presented in this study. In 2021, a monitoring system was installed in one of the main frames of two snow galleries built in the 1950s to follow up with temperature and displacements, including a trigger that sends out a warning message when a critical load is reached. A literature review on snow loads was performed, followed by calculations on snow distribution on the galleries based on the Eurocodes and National Swedish Standards. Finite element 2D and 3D models were created using AxisVM to accurately assess the efforts in the structural elements. Analysis and discussion are complemented by observations from site visits. It was concluded that the critical loads supported by the galleries are lower than the requirements of today’s standards, but since secondary construction elements were damaged before the main frames reached their full capacity, no major collapse has yet taken place. The cobweb effect (load re-distribution between the neighboring elements in a 3D structure) influenced the behavior of the galleries in the 3D analysis and the capacity of the main frames proved to be significantly increased compared to the 2D assessment.
Distinct and overlapping functions of YAP and TAZ in tooth development and periodontal homeostasis
Authors: Ma Jing , Fan Haixia , Geng Haixia
Published: January 2024

Orthodontic tooth movement (OTM) involves mechanical–biochemical signal transduction, which results in tissue remodeling of the tooth–periodontium complex and the movement of orthodontic teeth. The dynamic regulation of osteogenesis and osteoclastogenesis serves as the biological basis for remodeling of the periodontium, and more importantly, the prerequisite for establishing periodontal homeostasis. Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are key effectors of the Hippo signaling pathway, which actively respond to mechanical stimuli during tooth movement. Specifically, they participate in translating mechanical into biochemical signals, thereby regulating periodontal homeostasis, periodontal remodeling, and tooth development. YAP and TAZ have widely been considered as key factors to prevent dental dysplasia, accelerate orthodontic tooth movement, and shorten treatment time. In this review, we summarize the functions of YAP and TAZ in regulating tooth development and periodontal remodeling, with the aim to gain a better understanding of their mechanisms of action and provide insights into maintaining proper tooth development and establishing a healthy periodontal and alveolar bone environment. Our findings offer novel perspectives and directions for targeted clinical treatments. Moreover, considering the similarities and differences in the development, structure, and physiology between YAP and TAZ, these molecules may exhibit functional variations in specific regulatory processes. Hence, we pay special attention to their distinct roles in specific regulatory functions to gain a comprehensive and profound understanding of their contributions.
Topic modeling three decades of climate change news in Denmark
Authors: Meier Florian , Eskjær Mikkel Fugl
Published: January 2024

Climate change is a dynamic and rapidly evolving media agenda. First associated with scientific notions of the greenhouse effect, it was later presented as global warming before reaching the current and broader picture of climate change. Over its development, climate change reporting has touched on a broad range of topics reflecting shifting scientific understandings, political interventions, and public anxieties, all of which condition the public's view and actions on climate change. To better understand which issues the Danish public has been exposed to, this study uses topic modeling to analyse 32 years of climate change communication in Denmark (1990–2021, n = 63,743). It identifies 85 topics grouped into 14 themes dealing with climate change in Danish national media outlets. Topics differ in prevalence and longitudinal stability while reflecting outlet bias in political leaning and communicative modalities. The most pronounced differences in climate change reporting are between public service media and traditional newspapers. This indicates that media users relying mainly on online news from public service providers, without additional access to print media, will receive information on climate change that is more topical and less politicized, more thematic and less structural, more about high-level politics than everyday interventions and more concerned with consequences than solutions.
Coyote scat in cities increases risk of human exposure to an emerging zoonotic disease in North America
Authors: Raymond Sage , Steckler Deanna K. , Seguin M. Alexis , St. Clair Colleen Cassady
Published: January 2024

Introduction

Zoonoses associated with urban wildlife are increasingly concerning for human health and include the recent emergence of alveolar echinococcosis (AE) in North America. AE develops following infection with the tapeworm Echinococcus multilocularis. In Alberta, up to 65% of urban coyotes (Canis latrans) are infected with E. multilocularis, and infected scats contain eggs that can be accidentally ingested by people. Our goal was to determine the predictors of infection prevalence and intensity in coyote scats in Edmonton, Canada, and to identify the predictors of coyote scat deposition and content, especially as related to anthropogenic food sources and infrastructure.

Methods

To study infection prevalence and intensity, volunteers collected 269 scats, which were tested for E. multilocularis using polymerase chain reaction. We compared infection prevalence and shedding intensity by habitat and scat content. To determine predictors of scat presence and content, we used snow tracking to identify 1263 scats. We compared landscape characteristics at scats and available points, and among scats with different contents. We used negative binomial regression to predict scat abundance in city-delineated green spaces.

Results

26.0% of tested scats were positive for E. multilocularis (n = 70), and infection was twice as common as expected near compost and 1.3x more common than expected when scats contained anthropogenic food. Scats were more common than expected near other scats (80% within 1 m of scats, 27% at 11.5 m), buildings (19% at buildings, 16% at 80 m), and the camps of people experiencing homelessness (24% at camps, 20% at 60 m). Scats frequently contained fruit (52.9%), anthropogenic material (36.7%), and birdseed (16.0%), and scats containing anthropogenic material often occurred near human infrastructure, supporting a relationship between anthropogenic attractants and scat accumulation.

Discussion

These results suggest that abundant food sources and anthropogenic food increase coyote aggregation, increasing both scat abundance and infection rates, which in turn increases risk of exposure to zoonotic parasites for humans. Risk to humans might be reduced by preventing coyote access to anthropogenic and aggregated food sources and educating people who are likely to encounter infected soil or vegetation, including gardeners, park users, and people experiencing homelessness.
Circulating erythroferrone has diagnostic utility for acute decompensated heart failure in patients presenting with acute or worsening dyspnea
Authors: Appleby Sarah , Frampton Chris , Holdaway Mark , Chew-Harris Janice , Liew Oi Wah , Chong Jenny Pek Ching , Lewis Lynley , Troughton Richard , Ooi Shirley Beng Suat , Kuan Win Sen , Ibrahim Irwani , Chan Siew Pang , Richards A. Mark , Pemberton Christopher J.
Published: January 2024

Objectives

In dyspneic patients with atrial fibrillation (AF) or obesity, the diagnostic performance of NT-proBNP for acute heart failure is reduced. We evaluated the erythroblast derived protein erythroferrone (ERFE) as an ancillary biomarker for the diagnosis of acute decompensated heart failure (ADHF) in these comorbid subgroups in both Western and Asian populations.

Methods

The diagnostic performance of ERFE (Intrinsic Lifesciences) and NT-proBNP (Roche Cobas e411) for ADHF was assessed in 479 New Zealand (NZ) and 475 Singapore (SG) patients presenting with breathlessness.

Results

Plasma ERFE was higher in ADHF, compared with breathlessness from other causes, in both countries (NZ; 4.9 vs. 1.4 ng/ml, p < 0.001) and (SG; 4.2 vs. 0.4 ng/ml, p = 0.021). The receiver operating characteristic (ROC) areas under the curve (AUCs) for discrimination of ADHF were reduced in the NZ cohort compared to SG for ERFE (0.75 and 0.84, p = 0.007) and NT-proBNP (0.86 and 0.92, p = 0.004). Optimal cut-off points for ERFE yielded comparable sensitivity and positive predictive values in both cohorts, but slightly better specificity, negative predictive values and accuracy in SG compared with NZ. In patients with AF, the AUC decreased for ERFE in each cohort (NZ: 0.71, n = 105, SG: 0.61, n = 44) but increased in patients with obesity (NZ: 0.79, n = 150, SG: 0.87, n = 164).

Conclusions

Circulating ERFE is higher in patients with ADHF than in other causes of new onset breathlessness with fair diagnostic utility, performing better in Asian than in Western patients. The diagnostic performance of ERFE is impaired in patients with AF but not patients with obesity.
Investigating the role of hydrological connectivity on the processing of organic carbon in tropical aquatic ecosystems
Authors: Pereira Ryan , Panizzo Virginia N. , Bischoff Juliane , McGowan Suzanne , Lacey Jack , Moorhouse Heather , Zelani Noor Suhailis , Ruslan Muhammad Shafiq , Fazry Shazrul
Published: January 2024

Inland waters are highways of carbon and nutrient flows between the land and ocean. Aquatic environments integrate multiple sources and processes over space and time that influence ecosystem functionality. The complexity of these systems and their multiple interactions with the surrounding environment are conceptualised, but often lack empirical scrutiny that allows further understanding of how inland waters mobilise, transport, and utilise carbon and nutrients. This is particularly evident in tropical waters. Here, we apply advanced geochemical analyses of dissolved organic matter (DOM) composition in conjunction with algal pigment biomarkers, to determine the seasonal variability of organic matter production, processing and export for a tropical, floodpulse wetland, Tasik Chini (Malaysia). We identify two phases in the hydrological cycle: Phase 1 signifying a transition from the wet season with high suspended sediment and dissolved organic carbon concentrations. DOM is composed of humic substances, building blocks and lower molecular weight compounds. Towards the end this phase then are periods of increased water clarity and algal productivity. This is followed by Phase 2, which has a greater contribution of autochthonous DOM, composed of proteinaceous material, concomitant with lower dissolved nutrient concentrations, increased mixotrophic algae and emergent vegetation. Based on this framework, we highlight the role of such tropical wetland lakes as hydrological “bottlenecks,” through a lentic/lotic switch that shifts aquatic transport of carbon and nutrients from lateral river continuum supply to flood pulses. We highlight the need to consider inherent biases of spatial and temporal scaling when examining freshwater ecosystems along the land-ocean aquatic continuum.
Sociocultural factors, academic performance, and intercultural university education in the Peruvian Amazon
Authors: Morales Rocha José Luis , Coyla Zela Mario Aurelio , Serruto Medina Genciana , Vargas Torres Nakaday Irazema , Asencios Tarazona Vitelio , Arroyo Japura Gregorio , Huanca Frias José Oscar
Published: January 2024

The educational context in the Peruvian Amazon is moderately complex because of the interaction between students from 23 indigenous peoples, with a preponderance of Shipibo-Conibo, Ashaninka, Awajun, Shawi, Yanesha, Wampis, Quechua, and Cacataibo, among the most representative and in that order, with culturally different knowledge and worldviews. Thus, the university becomes a center of tolerance, recognizing and accepting the identity of others and coexisting with mestizo students from urban areas. This study aimed to determine the sociocultural and educational factors influencing academic performance in a virtual teaching context for intercultural university education in the Peruvian Amazon. A neural network model was applied to a sample of 162 students from a population of 1,835 students at an intercultural university in the Peruvian Amazon. Furthermore, 54% of the students are of mestizo origin, and 46% belong to the indigenous population. It was concluded that economic dependence on the family, failing midterm exams, feeling isolated and alone, and conflicts with a family member influence academic performance.
The influence of transmedia and extra-academic narratives on the formation of the historical culture of high school students
Authors: Muñoz-Muñoz Pablo , Ortuno-Molina Jorge , Molina-Puche Sebastian
Published: January 2024

This paper concerns with the importance of traditional and extra-academic sources of knowledge in the configuration of historical culture of Spanish adolescents. To conduct the research, a descriptive phenomenological approach has been designed by analyzing the responses of forty-eight students to a semi-structured interview in which they had to choose 5 characters of contemporary history and indicate the origin of the information. The results show the high coincidence about who are historical significant characters: males and politicians, coincident with other studies conducted in Spain and other countries of the near sociocultural context. The degree of coincidence can be considered as a homogeneous image of the historical culture of the participants. In that sense, there is a high rate of coincidence in the source where this vision of history is mainly generated: the history classroom. The information obtained leads us to confirm the need to reflect on the important stereotypes generated after many decades of formal history teaching with contents far from a necessary critical review. For this reason, it is necessary to reorient the teaching of history to provide a better critical reasoning about how the past is used to understand current societies (ontological perspective) along with how we know about the past (epistemological perspective).