bluuebunny/crossref_metadata_2025 huggingface dataset:
Umbrella Labyrinth Sedimentation Device Study
Authors: Wang Shuisheng
Published: December 2020

Umbrella-type mud-water diversion labyrinth sedimentation device, which is characterized by the combination of two swash plate and wing plate into an umbrella type with a set angle, the angle range of the two swash plate is 15º--100º, the vertical height of the swash plate is 60-70 mm, the parallel axis of the wing plate is the vertical direction, the height is 10-20 mm. the whole adopts the modular combination mode, and can be freely combined and disassembled according to different pond design, swash plate The interval (sedimentation distance) can be freely adjusted, mud take the mud road and water take the water road, to avoid the mutual interference of the water and mud road, to achieve the stability of the water quality. The turbidity of the sedimentation pond is low, stable in 0.3-2.0 NTU.
Discussion on Implementation and Supervision of Safety Production Responsibility of Construction Engineering Enterprises
Authors: Zhao Yan
Published: December 2020

In order to improve the safety production management ability of construction enterprises in an all-round way and implement the main responsibility of enterprise safety production, the government supervision department should play its own important role, adhere to the implementation of system requirements and supervision work principles according to the safety production responsibility, carry out overall planning and reasonable deployment for the safety production problems of construction engineering enterprises in many aspects. Through the continuous standardization of construction enterprise safety production work behavior, to curb the occurrence of all kinds of safety production accidents. This paper mainly stands in the perspective of the supervision department, studies and analyzes the implementation of safety production responsibility and supervision measures of construction engineering enterprises, for reference only.
Application of "3S" Technology in Land Resource Management
Authors: Xiao Jianshi
Published: December 2020

Land is the foundation of human existence, and it is also an important prerequisite and foundation for social construction and economic development. However, with the continuing construction and development of society and economy, a large number of trees have been felled, and the level of land desertification has been increasing. Therefore, it is very necessary to pay attention to strengthening land resource management. The application of "3S" technology in land resource management not only solves some problems in land resources, but also promotes the development of China's economy. The author conducted research and analysis on "3S" technology, and put forward an effective strategy for the application of "3S" technology in land resource management, hoping to help the smooth progress of land resource management activities.
Application of BIM Technology in Building Structure Design
Authors: Sun Fei
Published: December 2020

With the emergence of BIM technology, the design concepts and methods of building structures have also changed. For construction design work, it is necessary to reasonably control the various elements of building structure design and scientifically and rationally design the building structure to facilitate on-site construction. Based on this situation, the current BIM technology plays an increasingly important role in the process of building structure design and plays an active role in promoting optimal building structure design.
Effect of Intraperitoneal Administration of Paclitaxel Combined with Cisplatin in Treatment of Advanced Ovarian Cancer
Authors: Zhao Li
Published: December 2020

Objective: To analyze the effect of intraperitoneal administration of paclitaxel combined with cisplatin in treatment of advanced ovarian cancer. Method: Fifty-four patients with advanced ovarian cancer in our hospital were randomly selected from the beginning of July 2018 to the end of June 2019. The principle of grouping was based on double-blind randomization method. In experimental group, 27 patients were given intraperitoneal administration of paclitaxel combined with cisplatin. In control group, 27 patients were given intravenous administration of paclitaxel combined with cisplatin. Clinical data of the two groups were compared. Results: Short-term clinical efficacy and T lymphocyte subsets of experimental group were significantly improved when compared with control group. The difference was significant (P<0.05). There was no significant difference in adverse reactions between the two groups (P>0.05). Conclusion: The effect of intraperitoneal administration of paclitaxel combined with cisplatin is ideal for treatment of advanced ovarian cancer patients.
Significance of Psychological Nursing in oncology Internal Medicine
Authors: Liu Yilin
Published: December 2020

Abstract:Objective: To study and analyze the effect of using psychological care in patients with oncology. Methods: 66 patients with oncology internal medicine were collected and their psychological care was strengthened. The negative emotion score and quality of life score were observed and compared before and after nursing. Results: Patients had better negative emotional scores after care than before care; The scores of quality of life were significantly higher after nursing than before nursing (P <0.05). Conclusion: In the nursing process of oncology patients, strengthening psychological care can significantly improve the negative mood of patients, so that they can maintain good psychological condition and improve their quality of life, which is worth popularizing.
Clinical analysis on the differential diagnosis of gastrointestinal neoplasms by real-time endoscopic ultrasonography
Authors: Zhang Yumin
Published: December 2020

Abstract: objective: to study and analyze the effect of real-time endoscopic ultrasonography in the differential diagnosis of gastrointestinal tumors.Selection methods: between January 2014 and January 2015 xinyang 53 cases of digestive system cancer patients admitted in hospital, a total of 79 substantial tumor of the digestive system lesions, all patients were diagnosed by endoscopic ultrasonography real-time tissue elastography, use elastic imaging score, for patients with lesions and their surrounding structures control the elastic strain rate ratio of measure and comparison analysis.Results: compared with the malignant lesion group, the difference between the benign lesion group and the benign lesion group was statistically significant (P<0.05).The ratio of elastic strain rate in benign lesion group was lower than that in malignant lesion group (P<0.05).The diagnostic accuracy, sensitivity and specificity were 91.14%, 94.74% and 81.82% respectively.Conclusion: endoscopic ultrasound real-time tissue elastography is effective in the differential diagnosis of digestive system tumors, and can effectively determine the benign and malignant tumors and improve the diagnostic accuracy.
Analysis of the Effect of Anesthetics Comprising of Remifentanil Combined with Propofol During Thyroidectomy
Authors: Liao Qikai
Published: December 2020

Objective: To analyze the anesthetic effect of remifentanil combined with propofol during thyroidectomy. Methods: A total of 70 patients who underwent thyroidectomy during November 2018 to November 2019 in Chongqing Eleventh People's Hospital were selected and recruited. These patients were divided into control group and observation group by random number table method. Each group consisted of 35 patients. The patients in the control group was given intravenous anesthesia comprising of propofol combined with intermittent fentanyl anesthesia, whereas the patients in the observation group was given intravenous anesthesia comprising of remifentanil combined with propofol. Results: The heart rate and mean arterial pressure of the observation group after anesthesia for 30 minutes were lower than those of the control group, and the differences were statistically significant (P < 0.05). The total incidence of adverse reactions in the observation group was lower than that of the control group, and the differences were statistically significant (P < 0.05). Conclusion: Remifentanil combined with propofol is effective in thyroidectomy. This combination can reduce the risk of adverse reactions and maintain stable hemodynamic parameters.
Clinical Study of Xingnaojing Injection Combined with Craniocerebral Hypothermia Apparatus in the Treatment of Acute Hemorrhagic Stroke
Authors: Yue Xianfang , Zhou Hua
Published: December 2020

Objective: To investigate the clinical effect of Xingnaojing injection combined with craniocerebral hypothermia apparatus in the treatment of acute hemorrhagic stroke. Methods: Seventy-two patients with acute hemorrhagic stroke admitted to our hospital from March 2018 to September 2019 were randomly divided into two groups, with 36 cases in each group. The control group (n=36) was treated with craniocerebral hypothermia apparatus, and the observation group (n=36) was treated with Xingnaojing injection combined with craniocerebral hypothermia apparatus. The clinical efficacy, the National Institutes of Health Stroke Scale (NIHSS) score and the activity of daily living (MBI score) after 1 month of treatment were compared. Results: The effective rate of treatment in the observation group was higher than that in the control group (P < 0.05); NIHSS score was decreased in both groups (P < 0.05); MBI score was increased in both groups, and the change range of the observation group was greater than that of the control group, showing statistically significant differences (P < 0.05). Conclusion: Xingnaojing injection combined with craniocerebral hypothermia apparatus can improve the clinical efficacy, the neurological impairment and activity of daily living in the treatment of acute hemorrhagic stroke, which is worthy of clinical application.
Research on “Shared Health Care” Service Based on Queuing Model -- Optimization and Evaluation of Breast Cancer Screening of Primary Care
Authors: Fan Yayun , Li Cheng
Published: December 2020

With the application of queuing theory model, this paper regards breast cancer screening in primary health care service center as a queuing system. With the help of the tide of sharing economy, it puts forward a joint screening scheme based on M/M/s model under shared mode, and compares it with M/M/1 model of non-shared mode, shortens patient waiting time by sharing medical resources, plans existing resources reasonably, and enhances the comprehensive strength of primary health care system so as to optimize the screening of breast cancer at the grass-roots level.
The Clinical Effect of Two Approaches of Microsurgery in the Treatment for Pituitary Tumor
Authors: Chen Hui , Xue Feng , Chen Dengkui , You Chao , Peng Zongjun , Wu Xiaocong
Published: December 2020

Objective: To analyze the effect of two approaches of microsurgery in the treatment of pituitary tumor. Methods: The main body of this study was 69 patients with pituitary tumor who came to the hospital between December 2016 and December 2019. Taking the coin method as the standard, group A underwent nasal-sphenoid sinus approach with 36 cases; group B underwent transcranial approach with 33 cases. The treatment effects were compared. Results: The total effective rate of group A was 94.44%, and that of group B was 72.73%; the therapeutic index of group A was better than that of group B; the complication rate of group A was 8.33%, and that of group B was 30.30% (P<0.05). After treatment, the tumor volume of both groups decreased, and group A was smaller than group B (P<0.05). Conclusion: Nasal-sphenoid sinus approach for patients with pituitary tumors can improve the treatment index, enhance the curative effect, reduce the size of the tumor, and have better safety.
The Clinical Study of Multigene Combination Test to Guide Chemotherapy Combined with Targeted Therapy in Patients with Advanced Gastrointestinal Tumors
Authors: Zhang Zhisheng , Chen Gaoyang , Dai Qin
Published: December 2020

Objective: To study the clinical effects of multigene combination test to guide chemotherapy combined with targeted therapy in patients with advanced gastrointestinal tumors. Methods: The samples were selected from 60 patients with advanced gastrointestinal tumors admitted to our hospital from March 2019 to July 2020, and were divided into a study group and a control group using a random number table model; patients in the control group did not undergo genetic testing and FOLLOX4+PD-1 chemotherapy, while patients in the study group underwent TYMS, ERCC1, EGFR, and KRAS and VEGF gene expression levels test, and the sensitive treatment plan was determined based on the test results, and the clinical indexes were compared between the two groups. Results: By comparing the total effective rate, survival time, and time to disease progression of chemotherapy in the two groups, the study group has a significant advantage (P<0.05). Conclusion: The combination of chemotherapy and targeted therapy for advanced gastrointestinal tumor patients can improve the efficiency of chemotherapy and prolong the time of disease progression and survival, which is worthy of comprehensive promotion.
Screening of Biomarkers for Hypertension Susceptibility in Pregnancy
Authors: Xu Jian , Fan Liye , Qi Feng , Xiu Xia
Published: December 2020

Objective: To study the differential lncRNA / mRNA expression profiles of placental tissues in patients with gestational hypertension, analyze their possible mechanisms of action, and explore their target genes and small molecule drug-related lncRNAs. Methods: Three patients with gestational hypertension who were treated in our hospital from May 2018 to May 2019 were selected as the research subjects and three healthy pregnant women who underwent a prenatal examination in the same hospital were selected as the control group. The placental tissues were taken from the patients. RNA-sequencing was performed to construct lncRNA/mRNA differential expression profiles; screening differentially expressed lncRNAs were used to predict target genes, and GO and KEGG enrichment analysis predicted the biological functions of target genes and the enriched signal pathways, respectively. Protein-protein interaction network, lncRNA-miRNA-mRNA network, and differentially expressed gene-small molecule drug association networks were constructed. Results: RNA-seq analysis revealed 19 differentially expressed lncRNA (4 up-regulated; 15 down-regulated) (P<0.05). Moreover, 423 differentially expressed genes (DEGs) (84 up-regulated; 339 down-regulated)(P<0.05). GO and KEGG enrichment analysis found that gestational hypertension is mainly related to endothelial cell damage, inflammatory response, abnormal immune regulation, and abnormal trophoblast invasion. The PPI network and lncRNA-miRNA-mRNA network were constructed. Differentially expressed gene-drug small molecule prediction results found 19 pairs of differentially gene-small drug relationship pairs, mainly including antibody, inhibitor et al. Conclusion: Differently expressed lncRNAs in the placenta of patients with gestational hypertension can participate in the regulation of multiple biological functional level-related signal pathways through targeted regulation of their target genes, and play an important role in the occurrence and development of gestational hypertension. The predicted small molecule drug can be used as a reference for clinical treatment.
Review of the Methodical Manual “Methodology for Determining Whether a Blockchain Token Corresponds to a Digital Asset”, author Kud A. A.
Authors: Athavale Vijay Anant
Published: December 2020

Blockchain, so enthralling is that it ties in with technology, economics, and psychology. In this manual the author has explored it from a legal perspective, as well. Is that why people get so excited about blockchain – because there are so many different areas to think about? The fact that it is so multidisciplinary is actually more of a challenge for people than what gets them into it. Almost no one has the requisite expertise in all these different areas, so it leads to a lot of misunderstandings where people think they understand what’s going on, but only see one part of it. The manual describes the characteristics and indicators of a blockchain token to be considered as digital asset. Author has logically projected that digital assets may become universally recognised equivalent of value and have a legal tender status. In such a scenario this manual provides a credible tool to the practitioners in the field of finance to determine whether the characteristics of a blockchain token correspond to those of a digital asset. In the theoretical discussion author has beautifully explained the basic terms related to a blockchain token, to provide clarity at a glance, regarding the terms “digital asset”, “digital currency”, “virtual currency”, “cryptocurrency”, “virtual money”, “electronic money”. The analysis of all the terminologies have rightfully led to the conclusion that in most of the concerns digital asset means cryptocurrency. Author's approach in classifying the term digital asset with reference to the four components namely economic, legal, information and value is really commendable. The explanation is self satisfactory to showcase the huge potential of digital assets for real sector of economy and that's why there is a strong need for legal regulation. The methodology of principle of equivalence of categories proposed by the author to diagnose the equivalence of a blockchain token to a digital asset is fairly simple and reliable as all the nine categories “uniqueness”, “identifiability”, “regulability”, “sessionality”, “derivativeness”, “verfiability”, “transparency”, “decentralisation and “accountability” elaborated optimal characteristics to be at par with the existing international legal standards. A fairly simple and reliable mathematical formalization is there to determine that the diagnosed blockchain token corresponds to a digital asset. With all such incredible features the manual is really invaluable to all the practitioners’ as blockchain technology is rapidly changing landscapes of human life by bringing in efficiency both in terms of time and money. Inclusion of a thesaurus has really added a great value to the manual which provides conceptual clarity about the widely used terminologies in the context of blockchain technology.
Women, Mothers, and Monsters in Leïla Slimani's Novel "Dans Le Jardin de l'Ogre"
Authors: Yasmin Siti Aisyah , Udasmoro Wening , Sajarwa Sajarwa
Published: December 2020

Novel Dans le Jardin de l'Ogre (2014) karya Leïla Slimani mengandung aspek-aspek yang menjelaskan konstruksi seksualitas perempuan dengan penggambaran yang keluar dari pandangan mainstream yang normatif. Penggambaran perempuan sebagai mothers di satu sisi dan monster di sisi lain menjadi aspek yang ditinjau dalam penelitian ini dengan menggunakan lensa konsep women as mothers dan monsters karya Rossi Braidotti. Penelitian ini menggunakan metode analisis isi cerita (content analysis). Metode tersebut digunakan untuk dapat meninjau lebih jauh narasi yang terdapat di dalam novel sesuai dengan aspek-aspek yang ada pada teori. Data yang terdiri dari narasi dan dialog antar tokoh dikumpulkan dan dikategorikan sesuai dengan polarisasi perempuan sebagai mothers dan monsters. Teknik yang digunakan dalam penelitian ini adalah teknik simak catat. Hasil penelitian mengenai konstruksi seksualitas ini menunjukkan penginternalisasian nilai yang mendukung terjadinya konstruksi seksualitas perempuan di dalam novel Dans le Jardin de l'Ogre. Penginternalisasian nilai-nilai tersebut menyebabkan seorang perempuan harus menerima konsekuensi ketika ia menunjukkan seksualitasnya yang secara umum diposisikan keluar dari norma. Leïla Slimani's novel Dans le Jardin de l'Ogre (2014) examines the elements that influence the development and construction of female sexuality. Borrowing from the theories of Rossi Braidotti, aspects of motherhood and monstrosity are explored through this research. Content analysis is used as the research method, as it enables the convenient review of applicable and relevant data, i.e. the polarization between mothers and monsters. This study employs the notes technique as its research technique. It finds that, in Dans le Jardin de l'Ogre, women are depicted as internalizing values that support the dominant constructs of their sexuality. As a result, women face severe consequences if they exhibit sexuality that is considered as outside the norms.
Inisiasi Kunjungan Postnatal Care Dengan Tingkat Kesakitan Fisik Pada Ibu Pasca Melahirkan
Authors: Ibrahim Juliani
Published: December 2020

Postnatal services are a series of health services provided to mothers who have just given birth according to standars and performed at least three times according to the recommended schedule and implemented so far in primary, but the initiating of postnatal care visits is still low with what is expected and the high mortality and postpartum maternal morbidity is quite high in Indonesia due to vaginal infection and bleeding. The objectives of this study is to determined the relationship between postnatal care initiation and the  of mother's postpartum physical illness using observational analytic research design with a cross-sectional approach. More than 81 respondents, they are  postpartum mothers who had postnatal visits at least 3 months after giving birth. Data collection by filling the checklist and interview. Then data were analyzed using the chi-square test. As many as 69% of postpartum mothers who have birth spans of less than 2 years have the  postpartum infection and as many as 71.6% of postpartum mothers who are late for a postnatal care initiation also have the  postpartum infection. Less than 2 years of birth interval and late initiate of postnatal care vsisits are at risk of having a postpartum infection and are significantly associated with a high incidence of physicall ilness.
Uji Efektivitas Ekstrak Daun Tembakau (Nicotiana tabacum L.) terhadap Mortalitas Larva Aedes aegypti
Authors: Bestari Rochmadina Suci
Published: December 2020

Daun tembakau dapat digunakan sebagai larvasida karena memiliki kandungan alkaloid, saponin, polifenol dan minyak atsiri yang dapat mengakibatkan kematian larva dengan cara merusak sistem saraf dan sistem perncernaan larva. Penelitian ini bertujuan untuk mengetahui daya bunuh ekstrak daun tembakau (Nicotiana tabacum L.) dalam etanol 96% terhadap larva Aedes aegypti dan mengetahui konsentrasi yang efektif untuk membunuh larva. Penelitian ini bersifat eksperimental laboratorium murni yaitu memberikan perlakuan ekstrak daun tembakau (Nicotiana tabacum L.) terhadap larva Aedes aegypti dengan metode post test only controlled group design. Terdapat 600 Larva Aedes aegypti instar III secara acak dibagi menjadi 6 kelompok (0,025%, 0,050%, 0,075%, 0,1%, aquadest + CMC sebagai kontrol negatif serta abate sebagai kontrol positif). Penelitian ini menggunakan 4 pengulangan setiap kelompoknya diamati setiap 6 jam selama 24 jam. Berdasarkan hasil uji statistika non parametrik Kruskal-Wallis diperoleh nilai p = 0,000 yang artinya terdapat perbedaan efek larvasida yang signifikan antar kelompok. Berdasarkan hasil uji Post Hoc Mann-whitney, empat konsentrasi memiliki perbedaan yang signifikan dibandingkan dengan kontrol negatif, sedangkan jika dibandingkan dengan kontrol positif abate, konsentrasi 0,1% yang berbeda tidak bermakna pada jam ke-24. Hal ini menunjukkan bahwa konsentrasi 0,1% efektif membunuh larva Aedes aegypti. Ekstrak daun tembakau (Nicotiana tabacum L.) efektif dalam membunuh larva Aedes aegypti.
Penurunan Skala Nyeri Pasien Post-Op Appendictomy Mengunakan Teknik Relaksasi Genggam Jari
Authors: Wati Fitria , Ernawati Ernawati
Published: December 2020

Pasien post operasi insisi (penyayatan jaringan) mengalami nyeri dengan berbagai tingkatan Hampir 80% pasien post operasi pembedahan mengalami keluhan nyeri akut setelah pengaruh obat anastesi yang hilang, nyeri akan bertambah dengan adanya suatu peradangan atau infeksi, hal itu membutuhkan adanya suatu teknik perawatan untuk mengurangi nyeri salah satunya dengan teknik relaksasi genggam jari. Relaksasi genggam jari merupakan kombinasi antara relaksasi nafas dalam dan genggam jari-jari tangan, sensasi yang dirasakan memberikan persaan nyaman, sehingga mampu membebaskan mental dan fisik sehingga dapat meningkatkan toleransi terhadap nyeri. Studi ini untuk mengetahui pemberian terapi teknik relaksasi genggam jari dalam menurunkan skala nyeri pasien post op Appendictomy. Studi kasus ini menggunakan desain deskriptif dengan pendekatan Evidence Based Nursing Practice Jumlah sampel yang digunakan sebanyak 2 orang pasien post op appendectomy dengan kriteria yang sudah ditentukan dengan skala nyeri 3-6. Pengukuran skala nyeri mengunakan Numeric Rating Scale (NRS). Hasil perbandingan skala nyeri antara ke dua responden sebelum dan sesudah di lakukan terapi menunjukan penurunan skala nyeri. Responden 1 Hari ke-1: Selisihnya 1 (dari skala 5-skala 4), hari ke-2: Selisihnya 1 (dari skala 4-skala 3), hari ke-3: Selisihnya 1 (dari skala 3-skala 2).  Responden 2 Hari ke-1: Selisihnya 1 (dari skala 6-skala 5), hari ke-2: Selisihnya 1 (dari skala 5-skala 4), hari ke-3: Selisihnya 1 (dari skala 4-skala 3).  Terapi teknik relaksasi genggam jari dapat menurunkan skala nyeri pada pasien post appendectomy. Mekanismenya genggam jari sambil relaksasi nafas dalam mampu membebaskan ketegangan mental mental dan fisik dari ketegangan stress sehingga dapat meningkatkan toleransi terhadap nyeri.
Social prescription for those living with dementia; does MedTech have a role to play?
Authors:   1Master of Business Administration, Payame Nour University of Babol. 2Master of Translation, Shiraz Azad university.
Published: December 2020

Ageing is the major risk factor for dementia and nearly every country has seen its life expectancy rise from the beginning of the 21st century. Remaining socially connected has positive health and social implications and may be even more significant for marginalized group of people like those living with dementia. If appropriately used, social prescriptions can help deliver value-based social engagement and primary care by maximising the utilisation of resources and addressing social determinants of health, decreasing dependency on the biomedical model and thus providing a way for health care systems to deal with social determinants of health. More frequently, however, those seeking access to these programmes do not tend to do so simply due to lack of understanding and knowledge of the availability of such services. So, provision of social activities involves more than developing a program and hoping people will attend, and considering the particular situations of those living with dementia as marginalised group of people, and taking into account that there is no treatment for dementia, societies need to move toward social prescription, integrating appropriate MedTech support- targeting on those living with dementia- into such programs.
Metastatic tumors to the oral cavity – A retrospective analysis
Authors:   1G. Papanikolaou General Hospital, Thessaloniki, Greece. 2G. Papanikolaou General Hospital, Thessaloniki, Greece. 3Private OMF Surgeon, Lucerne, Switzerland. 4G. Papanikolaou General Hospital, Thessaloniki, Greece. 5Geneva University Hospital, Geneva, Switzerland. 6Theageneion Anticancer Hospital, Thessaloniki, Greece. 7G. Papanikolaou General Hospital, Thessaloniki, Greece.
Published: December 2020

Objectives: Metastatic tumors to the oral cavity are observed extremely rarely, accounting for approximately 1% of all malignant oral lesions. The purpose of our study is to record and analyze the data of the patients who revealed metastasis to the oral cavity. Material and Methods: The records of the patients diagnosed with oral metastases who were admitted to Oral and Maxillofacial Surgery Departments from 1996 to 2018 were reviewed and analyzed for demographic data and outcomes. Results: Over a period of 22 years (from 1996 to 2018), 22 patients were admitted to the Oral and Maxillofacial Surgery Departments of General Hospital G. Papanikolaou and Theageneion Anticancer Hospital with oral metastasic tumors from a distant primary site. Conclusions: Metastasis to the oral cavity is a very rare finding but it exists so we have to be aware of it and have in mind the possibility of this condition.
Printable Table of Contents: Informing Science Journal, Volume 24, 2021
Authors: Cohen Eli
Published: December 2020

Table of Contents for Volume 24 of Informing Science: The International Journal of an Emerging Transdiscipline, 2021
Culturable endophytic bacteria from garden pea (Pisum sativum L.)
Authors: Akhtemova G. A. , Vasileva E. N. , Afonin A. M. , Zhukov V. A. , Tikhonovich I. A.
Published: December 2020

From the organs of various genotypes of Pisum sativum L., culturable endophytic bacteria belonging to phyla Proteobacteria, Firmicutes, and Actinobacteria, were isolated. Among them, growth-stimulating strains were identified.
Characteristics of epiphytic microorganisms of wheat plants antagonists of phytopathogenic fungi
Authors: Aldosari M. , Ksenofontova Oksana
Published: December 2020

Epiphytic microbiological complex of the surface of spring wheat plants of the Saratov 70 variety was studied. Among of the selected epiphyte cultures, strains of fungicide producers were screened for the genera Alternaria, Aspergillus and Fusarium.
Growth-promoting activity of endophytic bacteria of the genus Bacillus
Authors: Alekseev Valentin Yu. , Veselova Svetlana V. , Sarvarova Elena R. , Maksimov Igor V.
Published: December 2020

Growth-promoting concentrations of the genus Bacillus new isolates and mixtures of endophytic strains of Bacillus subtilis were selected. Isolates B. subtilis Stl7 and Ttl2 are promising for the creation of biocontrol agents.
Selection of genetically marked maize lines for the ability to parthenogenesis
Authors: Apanasova N. V.
Published: December 2020

New genetically marked lines have been created. They could facilitate the transfer of parthenogenesis genes to other lines and serve as the basis for creating a collection of parthenogenesis donors.
Creation of a collection of microorganisms - destructors of organic substances that are promising for bioremediation of technogenic disturbed lands in Kazakhstan
Authors: Baigonusova Zh. A. , Rysbek A. B. , Kurmanbaev A. A.
Published: December 2020

The cultural-morphological and biochemical properties of 83 selected strains of organic substance destructor were studied. The analysis of nucleotide sequences of the genome of bacteria Bacillus cereus Fd 2 was performed.
Induction of somatic embryogenesis in different cotton cultivars and lines
Authors: Baimukhametova E. A. , Musin Kh. G. , Kuluev B. R.
Published: December 2020

During in vitro cultivation of various cultivars and lines of cotton, lines less prone to browning and forming healthy calli were revealed. The experiments on induction of somatic embryogenesis in these lines are conducted.
The effect of the biological product on the productivity of plants of the Solanaceae
Authors: Baubekova D. G.
Published: December 2020

A laboratory sample of a biological product has a pronounced phytostimulating activity and does not show a phytotoxic effect. The use of a sample in field experiments increases the yield of a tomato, improving its qualitative and quantitative indicators.
Response of plants and nitrogen-fixing symbiosis to the toxicity of cadmium and mercury using the pea mutant SGECdt
Authors: Belimov A. A. , Shaposhnikov A. I. , Syrova D. S. , Guro P. V. , Yuzikhin O. S. , Azarova T. S. , Sazanova A. L. , Gladkov G. V. , Sekste E. A. , Safronova V. I.
Published: December 2020

The combined effect of Hg and Cd on the growth, elemental composition, root exudation and interactions with rhizobia of pea SGE and its mutant SGECdt was studied in hydroponics and sand. The tolerance mechanisms of legume-rhizobia symbiosis to heavy metals are discussed.
An integrated approach to design of new biotechnological products to reduce man-induced load on environment
Authors: Belovezhets L. A. , Tretyakova M. S. , Markova Yu. A.
Published: December 2020

The regularities of transformation of complex organic substrates (oil, sawdust, hydrolysis lignin) by microbial associations have been established.
Bacteria of Pseudomonas genus in sugar beet agrocenosis
Authors: Bezler N. V. , Petyurenko M. Yu.
Published: December 2020

Bacteria of Pseudomonas genus able to fix nitrogen and to produce heteroauxin are developed on roots of sugar beet. Their isolation from rhizoplane and introduction onto surface of sugar beet leaf apparatus promotes development of aerobic spore-forming bacteria, ammonificators, and nitrogen immobilizators that activates growing of leaf blade growth, syntheses of sucrose and growth of beet roots.
Influence of chloride salinity on primary photosynthetic processes in potato leaves
Authors: Danilova E. D. , Коlomeichuk L. V. , Efimova M. V.
Published: December 2020

We investigated the effect of 125 mM NaCl on the potato leaves photosynthetic parameters (the content of chlorophylls a, b, and carotenoids; photochemical activity of photosystem II).
Search of phyto-toxicity microorganisms for weeds
Authors: Didovich S. V. , Pas’ A. N. , Alekseenko O. P.
Published: December 2020

Phyto-toxic strains for Ambrosia artemisiifolia, Amaranthus retroflexus, Cirsium arvense have been identified. The intensity of the lesion exceeded the control by 5-70 times (p < [0.0001-0.007]), depending on the strain and the location of the stomata.
Ion transporters in the root nodule of Medicago truncatula: potassium transporters
Authors: Fedorova E. E. , Trifonova N. A.
Published: December 2020

The transporter proteins were mistargeted and partly depleted from plasma membrane of mature infected cells, this phenomenon may contribute to the potassium loss by symbiosomes during their development and senescence.
Influence of bacteria of the genus Azospirillum on sugar beet productivity
Authors: Fedorova O. A. , Bezler N. V.
Published: December 2020

Spraying of sugar beet plants with nitrogen-fixing strains of bacteria of the genus Azospirillum promotes activation of sugar beet growth and increase of its productivity.
Microbiological quality of grain cultivated in the North Caucasus region
Authors: Gagkaeva T. Yu. , Gavrilova O. P. , Orina A. S. , Burkin A. A. , Khusaynov Kh. A.
Published: December 2020

The wheat and barley grain harvested in North Caucasus in 2019 were mostly infected by Alternaria (to 93%) and Fusarium (to 14%) fungi. The risk of contamination of grain with mycotoxins was shown.
Influence of chicken manure derived biochar on soil quality and wheat and barley growth
Authors: Galieva G. Sh. , Galitskaya P. Yu. , Selivanovskaya S. Yu. , Kuryntseva P. A.
Published: December 2020

Effects of two types of biochar made of chicken manure (at 300 and 700 peak temperatures) on soil biogenic elements content, microbial characteristics as well as barley and wheat growth were investigated.
SNP analysis of common wheat baking qualities
Authors: Galimova A. A. , Zaikina E. A. , Kuluev B. R.
Published: December 2020

The allelic states of waxy genes in genomes A, B and D were studied by genome-specific primers in cultivars and lines of common wheat of the pre-Ural steppe. The studied cultivars and lines revealed differences in genotypes for genome A.
Auxin and carotene biosynthesis by the bacterium Pantoea agglomerans
Authors: Gilvanova E. A. , Milman P. Yu.
Published: December 2020

Monitoring of auxin and carotene during cultivation of the Pantoea agglomerans strain IB-BF revealed that the maximum yield of the target products is provided not by population density, but by the qualitative composition of the nutrient medium and the need for a larger peptide component of the substrate (rich amino acid set), which is part of the standard LB medium.
The effect of copper on cadmium‐tolerant lawn grass
Authors: Gladkov E. A. , Gladkova O. N.
Published: December 2020

Cadmium‐tolerant plants were more resistant to copper. However, the increase in resilience was not very significant. Therefore, it is more expedient to obtain plants that are resistant to copper.
Novel determinants of plant-pectobacteria interaction: identification and characteristics
Authors: Gorshkov V. , Parfirova O. , Tsers I. , Petrova O. , Gogoleva N. , Ageeva M. , Islamov B. , Vorob’ev V. , Mikshina P. , Gogolev Yu.
Published: December 2020

There are “molecular switchers” that transform peaceful coexistence of plants with phytopathogenic pectobacteria to symptomatic infection. These switchers seem to be perspective “targets” for plant protection approaches.
Methodological approaches to agrobacterium-mediated transformation of buckwheat (Fagopyrum esculentum Moench)
Authors: Ilina E. L. , Kiryushkin A. S. , Guseva E. D. , Demchenko K. N.
Published: December 2020

The method of Agrobacterium rhizogenes-mediated transformation of buckwheat has been established; composite plants have been obtained. The distribution of the cellular response to auxin by reporter proteins with different maturation times coincides.
Intracellular transformations in bacteria as a response to external factors: molecular spectroscopic characterization
Authors: Kamnev A. A. , Tugarova A. V.
Published: December 2020

Examples are considered of using molecular spectroscopy techniques (Fourier transform infrared, Raman and Mössbauer spectroscopies) in monitoring the responses of bacteria to stresses.
Construction and characterization of the deletion mutant of slyA gene in phytopathogenic bacteria Erwinia amylovora
Authors: Koida E. S. , Lagonenko A. L.
Published: December 2020

The regulon of global transcriptional regulator SlyA in bacteria Erwinia amylovora was examined. For that, the ΔslyA mutant was constructed. The mutant showed a decreased level of amylovoran production and polymyxin B resistance.
Molecular mechanisms for the plant promoter efficiency correction
Authors: Komakhin R. A. , Efremova L. N. , Strelnikova S. R.
Published: December 2020

The mutations determining functional differences between two highly identical promoters from the chickweed plant were identified. These mutations lead to the formation of hypoosmolarity-responsive cis-element within core promoter and its specific positioning relative to the other cis-elements.
Correlation between the diversity of xenobiotic catabolism genes in Rhodococcus and phytotoxicity of imidazolinone and organophosphate herbicide biotransformation products
Authors: Kruglova M. N. , Chugunova Y. A. , Samkov A. A. , Volchenko N. N. , Khudokormov A. A.
Published: December 2020

Rhodococcus strains with a wide spectrum of catabolic genes, have provided a more marked reduction of toxicity of imidazolinone. In the case of glyphosate, the opposite strain-specific pattern is found.
Morphotypic assessment of autochthonous strains of Saccharomyces and non-Saccharomyces yeast on grape varieties Dostoinyy Anapo-Taman zone of viticulture
Authors: Lobodina E. V. , Al-Nakib E. A. , Avakimyan A. O.
Published: December 2020

The article presents the results of the assessment of morphotypes of strains of wine yeast sown from wort and pulp of samples of grape variety Dostoyny, taken in the SEC "Wine Village" of the Anapsky District of the Krasnodar Territory. The separation of saccharomycetes and non-saccharomycetes by morphotypic features and using an elective test was carried out.
Microbial preparation for soil bioremediation and crop yield increase
Authors: Mandrik-Litvinkovich M. N. , Orlovskaya P. I. , Kislushko P. M. , Kalamiyets E. I.
Published: December 2020

A microbial preparation based on bacteria with enzymatic, antimicrobial and growth-stimulating activities effectively reduces residual amounts of herbicides of sulfonylurea series and imidazolinones and promotes productivity of agricultural crops.
Changes in the taxonomic structure of the microbiome of chernozem southern of the rhizosphere Triticum aestivum L. under the influence of associative bacteria strains
Authors: Melnichuk T. , Egovtseva A. , Abdurashitov S. , Andronov E. , Abdurashitova E. , Radchenko A. , Ganotskaya T. , Radchenko L.
Published: December 2020

The influence of strains associated with Triticum aestivum L. has been established on the taxonomic structure of the rhizosphere of the southern сhernozem of Crimean Steppe. Seventeen dominant families and 126 families with a minor share are defined in the prokaryotic biome.
Genetic and phenotypic diversity of rare species of genus Iris L in the Southern Urals
Authors: Mikhaylova E. V. , Mustafina A. N.
Published: December 2020

We studied 11 populations of rare species Iris pumila and I. scariosa in Southern Urals. Sequencing of four different genetic markers was performed for the first time.
Microbial composition with the properties of plant growth promoter, biofertilizer and biological fungicide
Authors: Mikhailouskaya N. A. , Voitka D. V. , Yuzefovitch E. K.
Published: December 2020

Microbial composition A. brasilеnse+B. circulans+Т. longibrachiatum (MC) is effective inoculant for grain crops growing in erosion agrolandscaps. MC reveals the properties of plant growth promoter, biological fertilizer and biological fungicide. Poly functional positive action of three-component MC resulted in the increase of grain crops yield and improvement of its quality in stress conditions in erosion agrolandscaps.
Strain Aspergillus niger AM1 – a living organism resistant to white phosphorus
Authors: Mindubaev A. Z. , Babynin E. V. , Badeeva E. K. , Akosah Y. A.
Published: December 2020

Copper sulphate has no effect on the growth of aspergill in a media with white phosphorus. We compared the white phosphorus resistance of A. niger AM1 with three strains from the All-Russian Collection of Microorganisms. Highest resistance was observed in AM1.
Taxonomic diversity of lipolytic bacteria isolated at oil refineries in Krasnodar that is promising for bio-preparation
Authors: Moiseeva E. V. , Bobrikova L. P. , Karaseva E. V. , Khudokormov A. A. , Samkov A. A. , Volchenko N. N.
Published: December 2020

From the lipid-containing wastes, 20 strains of bacteria were isolated. According to MALDI, their taxonomic identity is established, the 13 most active lipolytic strains are classified as 8 genera, the dominant is the genus Pseudomonas.
Editing of genes coding protein involved in maize gamete membrane interaction and fusion
Authors: Moiseeva Ye. M. , Gusev Yu. S. , Volokhina I. V. , Fadeev V. V. , Mazilov S. I. , Chumakov M. I.
Published: December 2020

An agrobacterial transformation of maize was performed using a CRISPR/Cas9 vector for knockout the Zm_gex2 and Zm_gsc1 genes.
Amyloid proteins of plants and microorganisms: biological functions and participation in the formation of supra-organismal systems
Authors: Nizhnikov A. A.
Published: December 2020

Here we will review the latest advances in the study of functional amyloids of plants and symbiotic bacteria demonstrating the involvement of these protein fibrils in protein storage in plant seeds and formation of supra-organismal interactions.
Role of pathogen effectors SnTox in development of compatibility reaction in the pathosystem wheat – Stagonospora nodorum
Authors: Nuzhnaya T. V. , Veselova S. V. , Burkhanova G. F. , Rumyantsev S. D.
Published: December 2020

It was shown that the interactions of SnToxA-Tsn1, SnTox1-Snn1 in the pathosystem wheat-Stagonospora nodorum play an important role in the development of Septoria nodorum blotch and are aimed at manipulating of host redox-status.
The content of low molecular weight antioxidants in transgenic plants Nicotiana tabacum under heavy metal salts conditions
Authors: Pristipa K. V. , Kukulyanskaya T. A. , Khramtsova E. A.
Published: December 2020

We were studied several of antioxidants in transgenic Nicotiana tabacum, plants cultivated in heavy metal polluted soils. The content of phenolic compounds, vitamins C and E in plants increased under these conditions.
The effect of organic fertilizers on the taxonomic composition of microbial communities in agro-soddy-podzolic soils
of the middle taiga
Authors: Puhalsky Ya. V. , Loskutov S. I. , Lapteva E. M. , Vinogradova Yu. A. , Kovaleva V. A. , Perminova E. M.
Published: December 2020

The taxonomic composition of prokaryotes of agro-soddy podzolic soils of the middle taiga and the patterns of its change with the application of various doses of organic fertilizers were studied.
Role of the Stagonospora nodorum effector SnTox3 in regulation of cytokinins synthesis and metabolism in infected wheat plants
Authors: Rumyantsev S. D. , Veselova S. V. , Nuzhnaya T. V. , Burkhanova G. F.
Published: December 2020

The effect of the Stagonospora nodorum effector SnTox3 on the biosynthesis and metabolism of cytokinins of host plant was studied. The SnTox3 effector influenced the biosynthesis of cytokinins along an ethylene-dependent and ethylene-independent pathway.
Secondary metabolite profiles and biological activity of extracts from various isolates fungi Alternaria sonchi depending on the composition of the liquid nutrient medium
Authors: Salimova D. R. , Berestetskiy A. O.
Published: December 2020

Phytopathogenic fungus A. sonchi is able to produce metabolites with insecticidal properties. The composition of the culture media affected the metabolite profiles of the extracts. The results of the assessment of biological activity allowed to divide the working isolates with phytotoxic and insecticidal activity.
Anti-stress sugar beet cultivation technology
Authors: Sergeev V. S. , Mukminov D. R. , Minnebaev L. F.
Published: December 2020

During the growing season, sugar beets are subjected to various stresses, while most of the crop is lost. Biological products and biofertilizers produced by BashIncom reduce the impact of environmental stress factors and allow you to save the crop.
Biological efficiency of a complex microbial preparation against root rots of cereals in model and field experiments
Authors: Shmyga E. Yu. , Sidarenka A. V. , Sviridov A. V. , Korzhenevskij O. Ch. , Mandrik-Litvinkovich M. N. , Kuptsov V. N. , Kalamiyets E. I.
Published: December 2020

The influence of microbial preparation on the development of root rots in cereals was studied. It was found that the biological efficiency against root rot of oat in the model experiment was 65.0 %, and in the field experiment on winter triticale – 30.1-44.8 %.
Mycotoxin degradation by microbial metabolites
Authors: Statsyuk N. V. , Shcherbakova L. A. , Mikityuk O. D. , Nazarova T. A. , Dzhavakhiya V. G.
Published: December 2020

Extracellular metabolites of Gliocladium roseum GRZ7 are able to destroy aflatoxin B1 and zearalenone (by 61.9 and 68%, respectively). The determined optimum pH and temperature confirm the enzymatic nature of these metabolites.
Search for streptomycetes with antagonistic activity to the aggressive Fusarium sp. strain AC
Authors: Tovstik E. V. , Bakulina A. V.
Published: December 2020

The antagonistic activity of 126 Streptomyces strains against the fungus Fusarium sp. AC was studied. The greatest extent of mycelium growth was inhibited by S. geldanamicininus 3K9, which produces validamycin A.
Biopreparation "Vegetatin" for protection of cabbage from fungal and bacterial diseases during grows and storage
Authors: Trigubovich A. M. , Popov F. A. , Arashkova A. A. , Volchkevich I. G. , Kolomiyets E. I.
Published: December 2020

Effectiveness of usage biopreparation "Vegetatin" which is based on bacteria of the genus Bacillus to protect white cabbage from diseases was studied. Positive effect after treatments of seeds, seedlings and vegetative plants on the productivity and cabbage harvest has been established. Biological effectiveness of "Vegetatin" was at level of 48.9–53.6%, the stored yield –28.1 c/ha of cabbage heads. Processing of cabbage heads before storage reduced the damage of cabbage by mixed rots by an average of 30%.
Ionic transporters in the root nodule of Medicago truncatula: membrane antiporters Na+/H+
Authors: Trifonova N. A. , Korolyova M. I. , Fedorova E. E.
Published: December 2020

In root nodules of Medicago truncatula subjected to salt stress the level of expression of Na+/H+ antiporters NHX1 and NHX7 was estimated. The localization of NHX1 was studied by confocal and electron microscopy.