bluuebunny/crossref_metadata_2025 huggingface dataset:
PSV-7 Effects of music stimulus on behavior response, cortisol level and immunity horizontal of growing pigs
Authors: Li Jiafang , Han Qian , Zhang Runxiang , Liu Honggui , Li Xiang , Bao Jun
Published: November 2020

Enrichment environment is widely used to improve the welfare of domestic animals and satisfy their natural behavior. Music as an enriched environment can reduce abnormal behavior in humans, non-human primates and rodents. The aim of this study was to investigate the effects of repeated music stimuli on the behavior, physiology and immunity of growing pigs. A total of 72 5-week-old hybrid piglets (Large White × Duroc × Minpig) were randomly divided into three treatments groups of the music group (Mozart K.448, 65–70 dB), the noise group (mechanical noise, 85–90 dB) and the control group (silence, less than 40dB). During 60 days of auditory exposure, the behavioral responses, cortisol level and immune horizontal of the piglets were measured. The results showed that the active behavior of the growing pigs increased and lying behavior decreased in the music group compared to the control group (P < 0.05). The music stimulus increased tail wagging, tail in curl and playing behaviors (P < 0.05). The noise stimulus increased aggressive behavior (P < 0.05) but had no effect on the exploring and manipulative behaviors (P > 0.05). The frequency of the event behaviors decreased with the time of auditory stimuli (P < 0.05). Short-term music stimulus had a lower cortisol level than the noise and control groups (P < 0.05). Long-term music stimulus increased the level of IgG, IL-2 and IFN-γ (P < 0.05) and decreased the IL-4 level (P < 0.05). Noise stimulus reduced the level of IgG (P < 0.05) but had no effect on the level of IL-2, IL-4 and IFN-γ (P > 0.05). In conclusion, music stimulus triggers the pigs to show more positive behaviors, and the short-term music stimulus can reduce the stress response, while the long-term music stimulus can enhance the immune responses in the growing pigs.
PSIII-2 Assessment of runs of homozygosity and estimates of inbreeding in three purebred turkey (Meleagris gallopavo) lines
Authors: Adams Sarah M , Derks Martijn F , Makanjuola Bayode , Wood Benjamin , Baes Christine F
Published: November 2020

Runs of homozygosity (ROH) are continuous stretches of homozygous genotypes in an individual that have been passed down from a common ancestor and can be used to accurately characterize genomic data. These ROH are correlated with other measures of inbreeding and have been applied to quantify individual autozygosity. The aim of this study was to detect and describe ROH in the turkey genome and estimate and compare measures of pedigree-based inbreeding coefficients (FPED) and genomic-based inbreeding coefficients estimated from ROH (FROH) and the genomic relationship matrix (FGRM). Pedigree records (n = 2,616,890) and genotypic records (n = 6,371) were available from three purebred turkey (Meleagris gallopavo) lines. Genotypic records were collected between 2013 and 2019 and were obtained using a dense single nucleotide polymorphism array (56,452 SNP). The overall mean length of detected ROH per animal was 2.87±0.29 Mb and mean number of ROH per animal was 84.87±8.79. Short ROH with lengths of 1–2 Mb long were the most abundant throughout the genome, accounting for approximately 45% of the identified segments. Mean ROH coverage differed greatly between chromosomes and lines. Across all lines, genomic derived inbreeding coefficients (FROH=0.27; FGRM=0.32) were higher than coefficients estimated from pedigree records (FPED=0.14). Ranges of correlations between FROH and FPED (0.19–0.31), FROH and FGRM (0.68–0.73), and FPED and FGRM (0.17–0.30) were estimated. Results from the current research provide a fundamental description of inbreeding in the turkey genome which is critical considering the growing concerns of the detrimental effects of increased inbreeding on fitness and health in livestock production. The knowledge gained from this study may subsequently be used to evaluate and maintain genetic diversity that is necessary for genetic improvement and minimizing inbreeding in turkey breeding programs.
PSXII-25 Isolation and identification of bovine preadipocytes and screening of miRNAs associated with adipogenesis
Authors: Yu Xiang , Fang Xibi , Yang Runjun
Published: November 2020

Introduction: In the breeding of beef cattle, the adipogenesis is a significant process to fat deposition. Thus isolating and identifying bovine preadipocytes in vitro is a basis for investigating mechanism of adipogenesis. As important transcriptional regulators, miRNAs play a pivotal role in adipogenesis. Materials and methods: Bovine preadipocytes were isolated from subcutaneous fatty tissue and induced to differentiate into adipocytes. The verification of preadipocytes and adipocytes was performed by qPCR, Oil Red O stain and triglycerides detection. Total RNAs were extracted from bovine preadipocytes and adipocytes and proceeded small-RNA sequencing. KEGG pathway analysis and GO analysis were used to screen differently expressed miRNAs associated with adipogenesis. Results: The result showed that more lipid drops in induced group were observed and stained red compared with control group. The expression levels of key genes (pparγ, c/ebpα, fabp4, fasn and leptin) associated with adipogenesis were increased. Dlk1, as a marker gene of preadipocytes, was scarcely expressed in adipocytes. The content of cellar triglycerides detected in adipocytes was significantly upgraded. 131 miRNAs were found to be highly expressed in bovine adipocytes, and 119 miRNAs were highly expressed in bovine preadipocytes. KEGG pathway analysis showed that 4.76% of the differentially expressed genes were enriched in lipid metabolism. Discussion and conclusion: Previous studies have found that some miRNAs could regulate lipid metabolism. But few studies have explored the relationship between miRNAs and adipogenesis. In our study, we characterized the miRNAs associated with adipogenesis in bovine preadipocytes. The expression profiles of 11 miRNAs verified by q-PCR, including miR-149-5p, miR-199a-3p, miR-199a-5p, miR-148a and miR-449a were the same to sequencing data. Based on the KEGG pathway analysis, the predicted target genes were mainly enriched in signal transduction, catabolism and lipid metabolism. This work contributes to existing knowledge by providing an understanding of bovine adipogenesis at the molecular level.
PSVIII-5 Circulating serotonin (5-HT) concentrations are associated with failure of passive transfer in 3–5 day old dairy calves
Authors: Knott Gwyneth , Weaver Samantha , Hernandez Laura , Ollivett Theresa , Cramer Catie
Published: November 2020

Calf morbidity and mortality continues to be a challenge for the dairy industry and failure of passive transfer (FPT) is associated with an increased risk of disease. Serotonin (5-HT) has recently been implicated in the dairy calf immune system, but further work is needed to investigate the precise role of 5-HT in calf health. The objective of this study was to determine if 5-HT serum concentration at 3–5 days of age was associated with FPT. Blood samples were collected from 3–5 d old dairy calves (n = 418). Blood serum was assessed for FPT (serum protein < 5.5 g/dL) and analyzed using a commercial ELISA kit to determine circulating 5-HT concentrations. The mean (± SD) 5-HT concentration was 2574 (±1007) ng/mL. A logistic regression was used to determine if FPT (outcome; n = 124/418) was associated with 5-HT concentration (predictor; low < 2502 ng/mL, high= ≥ 2502 ng/mL). The model controlled for sex, breed (Holstein or Jersey), colostrum type (replacer or pasteurized), and dystocia (hard pull/surgical delivery). Sex, colostrum type, breed, and dystocia were not associated with FPT (P > 0.05). Calves with low serum 5-HT concentrations had a 2.7 (95% CI: 1.2–7.1) greater odds of having FPT compared to calves with high serum 5-HT concentrations (P = 0.04). Higher serum 5-HT concentrations were associated with successful passive transfer, but the causal pathway is unknown. Therefore, further investigation regarding 5-HT at different time points during calfhood should be explored as a potential way to reduce FPT in neonatal dairy calves.
PSVIII-10 Effects of Folia Betulae and Méntha piperíta extracts on microbiological and enzymatic characteristics of cattle rumen
Authors: Makaeva Ajna , Atlanderova Kseniya , Duskaev Galimzhan , Nurzhanov Baer , Rysaev Albert
Published: November 2020

Refusal of antibiotics and a decrease in the greenhouse effect allows active study of the effectiveness of medicinal plants in order to increase the productivity of cattle. The aim of this study was to evaluate the effect of Folia Betulae (FB) and Méntha piperíta (MP) extracts (0.5 ml/kg body weight) on rumenal microbiota and fermentation characteristics against the background of control (C). They were added separately as a substrate to the diets of bulls with rumen fistula (dairy breed, 12-month, diet - 60% hay, 40% barley grain within 10 days). Fermentation parameters (CH4, volatile fatty acids, grain dry matter digestibility (DM) were analyzed daily, and microbiota of archaea and bacteria were analyzed using highly efficient 16S rRNA gene sequencing. FB and MP were prepared by grinding, extraction in a water bath (30 min, 70°C) and filtration. The results of this study showed that the formation of propionate and digestibility of DM did not differ compared to C. Methanobacteria in MP had lower values (P ≤ 0.05) compared with FB and C (less than CH4). Bacterial communities differed: Bacteroidetes predominated more in MP (P ≤ 0.05) and further in FB (P ≤ 0.05) compared with C. At the family level, Prevotellaceae dominated in MP (P ≤ 0.05), Bacteroidales in FB (P ≤ 0.05)) and Porphyromonadaceae in MP and FB (P ≤ 0.05). Firmicutes had lower values (P ≤ 0.05) in all cases compared to C, Clostridia class was lower by 6.0–8.4% (P ≤ 0.05) (due to Clostridiales and Lachnospiraceae families). This study emphasizes the potential use of herbal medicinal substances as a natural feed supplement, which can play a role in reducing methane formation and the development of gram-positive bacteria, without adverse effect to ruminal microbiota. This research was performed with financial support from the RSF (16-16-10048) and project 0761-2019-0005.
PSI-26 The Effects of Probiotics on Growth and Metabolism in Ram Lambs
Authors: Trench Alexis M , Smith Brandon I , Feyler Jenna E , Aborn Sharon L , Reed Sarah A , Zinn Steven A , Govoni Kristen E
Published: November 2020

Probiotic treatment can improve feed efficiency, increase growth, and alter blood components in small ruminants. Several commercial probiotics are available, but they vary in microorganisms resulting in different effects on growth and health. We hypothesized that Probios (Menomonie, Wisconsin) powder would increase growth, feed efficiency, and alter blood metabolites in ram lambs from 2 to 6 months of age. Twenty individually housed Shropshire ram lambs (average age 59.85 ± 2.74 days) were randomly assigned to a control or probiotic treatment group. Lambs were fed lamb concentrate (17.2% CP, 3.56 Mcal/kg) at 3.6% of their body weight and ad libitum hay (16.2% CP, 2.53 Mcal/Kg). Probios (10g) was fed once daily in concentrate of the probiotic group. Feed intake (FI) was determined daily and body weight (BW), body condition score (BCS), feed:gain (F:G), and average daily gain (ADG) were determined weekly. At weeks 0 and 16, heart girth (HG) and crown-rump length (CRL) were measured and ultrasonography of back fat (BF) and loin-eye area (LEA) was performed. Blood samples were collected biweekly. Week 16 blood metabolites were determined using Food Animal Maxi Panel (Missouri Veterinary Medical Diagnostic Laboratory). Plasma concentration of malondialdehyde (MDA) was determined at weeks 0, 6, and 16. Data were analyzed using SAS. Statistical significance was considered at P≤ 0.05. As a result of time, BW, BCS, ADG, FI, and F:G increased for all animals (P < 0.0001). No effect of treatment was observed for growth, feed efficiency, or oxidative stress measurements (P ≥ 0.33). Plasma albumin concentration increased by 12.66% (P = 0.02) and magnesium concentration increased by 5.83% (P = 0.01) as a result of treatment. Treatment had no effect on other measured blood metabolites. Probios powder did not alter growth but did alter blood metabolites in these lambs. Future studies should examine more effects of Probios on metabolism in growing lambs.
PSIV-27 Peripheral complete blood cell count results in pigs are impacted by gender, sow parity, and farrowing season
Authors: Rottman Elle , Watson Alisun N , Buck Catherine , Tsai Tsungcheng , Chewning Jeffery J , Maxwell Charles V , Kegley Elizabeth B , Powell Jeremy G
Published: November 2020

Complete blood cell counts have been used as a diagnostic tool across many animal species including swine. To investigate the factors that cause variation in complete blood cell count results, a total of 2,284 whole blood samples were collected from 2012 to 2019 in preweaning piglets (n = 518), nursery pigs (n = 1,704), and grower pigs (n = 60). Whole blood was collected into K2EDTA blood collection tubes and assayed using an automatic hematologic analyzer within 6 hours of collection. Data were analyzed by Mixed procedure of SAS with gender, parity group, and farrowing season as fixed effects. Body weight and age of pigs served as covariances. Farrowing season was grouped into summer (born during May to October) or winter (or November to April). Pigs that were born from first, second, and third parity, and four and above parity sows were assorted into parity group 1, 2 to 3, and 4+, respectively. Barrows had a greater concentration of total white blood cells (P < 0.01), lymphocytes (P < 0.01), and neutrophils (P < 0.01) compared to gilts. Barrows had lower mean corpuscular volume (P = 0.03), mean corpuscular hemoglobin (P < 0.01), and mean corpuscular hemoglobin concentration (P = 0.02) compared to gilts. Pigs that were farrowed in the winter season had a greater concentration of white blood cells (P = 0.01), neutrophils (P = 0.01), and the percentage of neutrophils (P = 0.03), but were lower in the percentage of lymphocytes (P = 0.03) compared to pigs farrowed during summer. Pigs born to parity four and above sows obtained a greater lymphocyte count (P = 0.01), percentage of neutrophils (P = 0.02), and percentage of lymphocytes (P = 0.01). We concluded that peripheral complete blood cells count results were affected by gender, farrowing season, and sow parity.
PSVII-24 Weight at maturity of water buffaloes determined by body chemical composition
Authors: Aranha Aline S , Castilhos André M , Francisco Caroline L , da Silva Daiane C Marques , Jacaúna Amanna G , Meirelles Paulo R L , Jorge Andre M
Published: November 2020

This study determined the weight at maturity (WM) of water buffaloes of three genetic groups (GG:Jafarabadi, Mediterranean, and Murrah) slaughtered at different weights (420, 480, and 540 kg). One-hundred-fifty-three non-castrated male (means of initial body weight and age of 314 ±117 kg and 390 ±32 days, respectively) from two similar experiments were used. The animals were slaughtered and the non-carcass components of each animal were weighed to obtain the empty body weight (EBW). The left half-carcasses were dissected into muscle, fat, and bone. Each tissue of each animal was ground separately and subsequently aliquoted for further analyses. The determination of moisture, crude protein (CP), ether extract (EE), and ash was performed by the classical method. Nonlinear regressions were used to predict the body composition of water, CP and ash. An exponential model was used to adjust the data for the content of the EE. Random coefficient models were used to identify fixed and random effects using the NLMIXED procedure in SAS. The genetic group was tested as fixed effect and year as a random effect. Body CP was determined based on CP fat-free dry matter (CPFFDM). The equations predicted the body composition of CP, EE, water and ash, and the results were expressed as a percentage of the EBW. WM was estimated when the non-significant increase in CPFFDM was no longer observed. There was effect of GG for WM model (P = 0.037), thus the WM was determined for each GG. The models suggest that Jafarabadi reach maturity at ~505.93kg of EBW [WMCPFFDM=79.279×(1–1.17×e-0.010×EBW)]; Mediterranean reach maturity at ~494.40kg of EBW [WMCPFFDM=79.186×(1–1.18×e-0.011×EBW)]; Murrah reach maturity at ~423.79kg of EBW [WMCPFFDM=78.793×(1–11.99×e-0.018×EBW)]. In conclusion, the WM of water buffaloes is dependent on the GG reaching 505.93, 494.90, 423.79 kg of EBW for the Jafarabadi, Mediterranean, and Murrah genetic groups, respectively.
PSXI-31 Program Chair Poster Pick: Determination of the threonine requirement in adult and senior Labrador Retrievers using the indicator amino acid oxidation technique
Authors: Weil Jordan T , Varney Jessica L , Fowler Jason W , Coon Craig N
Published: November 2020

Although nutrient profiles for canines have been developed in the past, the need to update amino acid (AA) requirements has gained importance as genetic selection changes the recommended nutrients. Correctly feeding AA to canines can have enormous effects, considering a deficiency or excess of such nutrient can lead to weight loss, disease, or in some cases, death. Amino acid requirements can be determined through the nitrogen balance or indicator amino acid oxidation (IAAO) methods. In this experiment, the IAAO technique was used to determine the threonine (Thr) requirement in Labrador retrievers. A total of six dogs (6 adult and 6 senior) were subjected to six diets with varying levels of Thr, ranging from deficient to excess. Diets were formulated to 1.6x NRC values for all indispensable amino acids. The control diet was fed for two days, followed by a day in which the test diet was fed, a tracer AA was supplied, and breath samples were collected. On test day, a priming dose of L-[1-13C]phenylalanine (Cambridge Isotope Laboratories, Inc.) based on the subject’s body weight was first supplied, followed by [1-13C]Phe doses every thirty minutes, spanning a four hour period. A respiration mask was placed on each subject every thirty minutes (Oxymax, Columbus Instruments), 13CO2 was collected, and enrichment was determined by isotope ratio mass spectrometry (IRMS). Results for IRMS were converted to atom percent excess (APE) and analyzed using a piecewise model of best fit (JMP® Pro 15). The segmented line regression showed that the Thr mean and population requirements were determined to be 1.21 ± 0.24 and 0.92 ± 0.17 g/1000kcal (mean ± 2SD) for adult and senior dogs, respectively. As the pet food industry becomes more specialized in diets relating to aging, and diseased canines, updating the amino acid requirements related to such animals is increasingly important.
PSIX-24 Comparison of serial real-time A-mode vs. B-mode ultrasound fat depth measures on prediction of final carcass value endpoints
Authors: Dockray Chandler A , Perkins Tommy L , Riberio Flavio , Kirkpatrick Tylo J , Wesley Kaitlyn R , Pillmore Sierra L , Cooper Kimberly B , Garrison Max D , Tennant Travis , Nichols Wade , Hutcheson John , Lawrence Ty
Published: November 2020

Charolais x Angus steers (n = 80) were evaluated across a 378-day feeding period in response to administration of a growth promoting implant containing trenbolone acetate (TBA) and estradiol (E2) using A-mode and B-mode ultrasound. Steers were scanned at 42-d intervals (0, 42, 84, 126, 168, 210, 252, 294, 336) during 10 separate ultrasound scanning sessions 24-h prior to harvest. Ultrasound B-mode rump fat images (BRFU) were obtained by a certified Ultrasound Guidelines Council (UGC) technician using an ALOKA 500V console equipped with a 17.2 cm carcass probe. Ultrasound A-mode images (ARFU) were obtained by a non-UGC certified technician using a RENCO Lean Meater. Images of BRFU were analyzed by the CUP lab whereas ARFU image results were determined chute side by the scanning technician. Carcasses were dissected into total lean, fat and bone post-harvest. Rump fat thickness ultrasound measurements were compared to carcass traits used to determine carcass value. Carcass endpoints included 12th–rib fat thickness (FTC), 12th rib-eye area (REAC), marbling score (MARB), and total percentage of carcass fat (PCF). The correlation between BRFU and ARFU was 0.70 (P < 0.01). Positive correlations (P < 0.01) between ultrasound rump fat measures taken the day before harvest and carcass measurements indicated moderate relationships between BRFU and FTC (r=0.80); BRFU and REAC (r=0.66); BRFU and MARB (r=0.68); BRFU and PCF (r=0.75); ARFU and FTC (r=0.69); ARFU and REAC (r=0.76); ARFU and MARB (r=0.79); and ARFU and PCF (r=0.80). These results indicate A- and B-mode ultrasound measures of rump fat may be adequate measures for estimation of total percent carcass fat.
PSIX-29 JAK2-STAT3 Pathway Mediated Satellite Cell Apoptosis to Govern Skeletal Muscle Growth with Lysine
Authors: Song Zhi-wen , Jin Cheng-long , Ye Mao , Gao Chun-qi , Yan Hui-chao , Wang Xiu-qi
Published: November 2020

Apoptosis is programmed cell death that can be stimulated by external stress or nutrition restrictions. Lysine (Lys) is an essential amino acid for pig growth, and the relationship between Lys deficiency caused apoptosis and inhibition of skeletal muscle growth remains unknown. The objective of this study was to investigate whether apoptosis could be regulated by Lys supplementation and the potential mechanism. In current work, 30 male Duroc × Landrace × Large weaned piglets were divided randomly into 3 groups: control group (Lys 1.30%), Lys deficiency group (Lys 0.86%), and Lys rescue group (Lys 0.86%, 0-14d; 1.30%,15–28 d). The experiment lasted for 28 days, and on the morning of 29 d, piglets were slaughtered to collect samples. Isobaric tag for relative and absolute quantification (iTRAQ) proteomics analysis of the longissimus dorsi muscle showed that Janus family tyrosine kinase (JAK)-signal transducer and activator of transcription (STAT) pathway was involved in Lys deficiency-induced apoptosis and inhibited skeletal muscle growth. Meanwhile, western blotting results of the longissimus dorsi muscle demonstrated that Lys deficiency caused apoptosis (P < 0.05) with the JAK2-STAT3 pathway inhibition (P < 0.05). Interestingly, apoptosis was suppressed (P < 0.05), and the JAK2-STAT3 pathway was reactivated (P < 0.05) after Lys re-supplementation in longissimus dorsi muscle. In addition, results of satellite cells (SCs) isolated from the longissimus dorsi muscle of 5-day-old Landrace piglets showed that Lys deficiency-induced apoptosis (P < 0.05) was mediated by the JAK2-STAT3 pathway inhibition (P < 0.05). Moreover, the JAK2-STAT3 pathway was reactivated (P < 0.05) by Lys re-supplementation and suppressed apoptosis in SCs (P < 0.05), and this effect was blocked (P < 0.05) after SCs treated with AG-490 (a specific inhibitor of JAK2). Collectively, Lys inhibited apoptosis in SCs to govern skeletal muscle growth via the JAK2-STAT3 pathway.
PSVIII-35 Late-Breaking Abstract: Administration of an appeasing substance at castration improves performance of pre-conditioned beef crossbred steers
Authors: Schubach Kelsey , Cappellozza Bruno I , Burim Marcos Renato , Velasco André , Sagrado Fernando , Bastos João Paulo , Cooke Reinaldo F
Published: November 2020

Alternatives that alleviate stress-related responses and improve performance of the beef cattle herd are imperative and deserve further investigation. In previous trials, administration of the bovine appeasing substance (BAS) has improved performance of newly-weaned and feedlot received beef animals, as well as reduced the risk of dark, firm, and dry (DFD) cuts in carcass obtained from Bos indicus cattle. Therefore, this study was designed to evaluate the effects of BAS administration at castration on pre-conditioning performance of crossbred beef animals. On day 0, 390 crossbred Angus × Nellore animals were ranked by initial shrunk BW (255 ± 21.1 kg) and assigned to receive or not (CON) 5 mL/head of BAS (SecureCattle; Nutricorp, Araras, SP, Brazil) immediately before castration. From d 0 to 30, animals within treatments were maintained in 2 feedot pens with a minimum distance (300 m) to avoid contact between treatment groups and received a grass hay-based diet (70:30 roughage:concentrate ratio) ad libitum. On day 30, animals were individually weighed following 16 hours of feed and water restriction. Hence, a descriptive analysis was performed to evaluate pen dry matter intake (DMI) and feed efficiency (FE). Animal was considered the experimental unit and all data were analyzed using the PROC MIXED procedure of SAS (version 9.4; SAS Inst. Inc., Cary, NC). Bovine appeasing substance administration at castration improved ADG and BW change (P < 0.0001) compared with CON cohorts (0.983 vs. 1.155 kg/d and 29.5 vs. 34.6 kg for ADG and BW change, respectively). Total DMI was similar among treatments when reported as kg/d (6.70 vs. 6.75 kg) or % of BW (2.48 vs. 2.48%), whereas FE was dramatically improved in BAS-administered animals (146 vs. 172 g/kg for CON and BAS, respectively). In summary, BAS administration improved performance of crossbred beef animals for 30 days post-castration.
PSX-32 Late-Breaking Abstract: Production of a Gene Knock-In Bull Calf by Embryo-Mediated Genome Editing
Authors: Owen Joseph R , Henning Sadie L , McNabb Bret R , Lin Jason C , Young Amy E , Murray James D , Ross Pablo J , Van Eenennaam Alison L
Published: November 2020

Genome editing offers an opportunity to introduce targeted gene insertions into livestock breeding programs. Molecular geneticists have typically employed a donor repair template and the homologous recombination (HR) pathway in somatic cells to introduce gene knock-ins into livestock genomes, followed by cloning. Editing embryos directly to achieve targeted gene knock-ins is inefficient, especially for introducing large DNA sequences. Here we report using a one-step method to produce a gene knock-in bull calf by cytoplasmic microinjection of CRISPR/Cas9 reagents into a bovine embryo. In vitro fertilized one-cell bovine zygotes were injected with a gRNA/Cas9 ribonucleoprotein complex and homology mediated end joining donor template containing the sex determining region Y (SRY) gene, the green fluorescent protein (gfp) reporter gene driven by the SV40 promoter, and one kilobase homology arms targeting the H11 safe harbor locus on bovine chromosome 17. Seven-day blastocysts were evaluated using fluorescent microscopy, and nine green fluorescent embryos were transferred to synchronized recipients. Ultrasound evaluation at 35 days revealed one pregnancy. In April 2020, a healthy 50 kg male calf was born. DNA was extracted from placenta, blood and a fibroblast line derived from the calf and analyzed for SRY-GFP knock-in, as well as genotypic sex. PCR and Sanger sequencing revealed the biallelic edit of the target location on chromosome 17, with the insertion of three or seven copies of the SRY-GFP construct in addition to donor plasmid backbone, or a 26 base pair insertion, and an XY genotype. Future analysis of the XX offspring inheriting the SRY gene on chromosome 17 from this knock-in bull will reveal whether inheritance of the bovine SRY gene is sufficient to trigger the male developmental pathway in cattle.
PSV-33 Studies of heterocyclic aromatic amines accumulation in meat products, depending on the raw materials type and the heat treatment duration
Authors: Utyanov Dmitry A , Kulikovskii Andrey V , Knyazeva Alexandra S , Kuznecova Oxana A
Published: November 2020

Steaks weighing 150.0±1.0 g and 2.5 cm thick were formed from chicken, pork and beef. After steaks formation they were fried using electric grill in temperature 230°C. First set of steaks were fried during 5 mins on each side, second one – during 7.5 mins, and the third set were fried during 10 mins on each side. After frying, steaks were tested for 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-Amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) content. Analysis of HAAs was performed by HPLC system (Agilent 1200) with QQQ MC/MC detector (Agilent 6410B). The studies did not show a large difference in the total concentration of HAAs formed during frying of pork and beef, but the amount of HAAs in chicken is 1.5–2 times less than in pork or beef. This may be caused by the lowest fat content in poultry meat relative to other types of meat. At the same time, an increase in the duration of heat treatment by 2.5 minutes on each side increases the amount of HAA formed in the product almost by 1.5 times. Total amount of HAAs detected in fried chicken: 22.45, 35.76 and 54.36 ng/g for 10, 15 and 20 minutes of heat treatment duration. Total amount of HAAs detected in fried pork: 47.09, 73.68 and 112.39 ng/g for 10, 15 and 20 minutes of heat treatment duration. Total amount of HAAs detected in fried beef: 42.56, 61.91 and 92.21 ng/g for 10, 15 and 20 minutes of heat treatment duration. The results of HAAs content are shown in table 1. Research conducted as part of the state assignment of Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences 0585-2019-0007.
PSIII-34 Standardized ileal digestible lysine requirements for 7- to 15-kg weanling pigs with high-lean genetics fed a corn-soybean meal-based diet
Authors: Lee Jinyoung , González-Vega Caroline , Htoo John K , Nyachoti Charles Martin
Published: November 2020

Continued genetic improvement necessitates the verification of nutrient requirements for newly developed pig genotypes. Therefore, the objective was to determine the standardized ileal digestible (SID) lysine requirements for 7- to 15-kg high-lean piglets (TN Tempo × TN70) fed a corn-soybean meal-based diet. A total of 144 piglets (6.51 ± 0.56 kg BW) were assigned to 1 of 6 diets in a randomized complete block design based on BW to give 8 replicate pens each with 3 pigs over a 21-d period. Sex was balanced as 4 replicates for each male and female. Diets contained 1.00, 1.16, 1.32, 1.48, 1.64, or 1.80% SID lysine, achieved by adding crystalline L-Lysine·HCl at the expense of cornstarch. Other indispensable AA were provided to meet the requirements. Individual BW and feed disappearance were recorded weekly and blood was sampled on d 1, 14, and 21. Data were analyzed using MIXED procedure of SAS including the SID lysine concentration as the fixed effect and the block as the random effect. Sex effect was not significant, thus was excluded from the model. The SID Lys requirements were estimated for linear and quadratic broken-line using NLIN procedure of SAS. Lysine content did not affect ADG and ADFI during wk 1, but quadratically increased (P < 0.05) G:F. From d 14 to 21, ADG and G:F increased (P < 0.05) quadratically by increasing dietary lysine content. Overall, increasing dietary lysine content quadratically increased (P < 0.05) ADG and G:F; whereas, plasma urea nitrogen quadratically decreased (P < 0.05). In conclusion, the SID lysine requirements for optimal growth performance of 7- to 15-kg piglets fed corn-soybean meal-based diets based on linear and quadratic broken-line models were 1.27 and 1.38% for ADG and 1.36 and 1.46% for G:F, respectively, thus giving an overall average value of 1.37%.
PSII-32 In vitro effect of growth hormone on progesterone production by large preovulatory follicles depends on the hen age and follicular layers interaction
Authors: Smekalova Araksiya , Montvila Elena K , Konovalova Olga , Mityashova Olga , Lebedeva Irina
Published: November 2020

Growth hormone (GH) is an endocrine and paracrine/autocrine regulator of the hen ovarian function, with the GH receptor concentration in the granulosa layer (GL) being maximum in the largest preovulatory follicle (Lebedeva et al. 2004, Biol.Reprod. 71:1174–1181). In the present study, GH effects on in vitro production of progesterone by GL from the two largest yellow follicles (F1 and F2) were investigated due to the hen age and the presence of the theca layer (TL). Young hens with long clutch (YLC, 32–33 week-old, >10 eggs per clutch) and old hens with short clutch (OSC, 74–76 week-old, 3–6 eggs per clutch) were used. After isolation, GL from F1 and F2 follicles (n = 8–9) was cultured separately or jointly with the respective TL for 18 h in the presence or absence of chicken GH (25 ng/ml). Concentrations of progesterone in culture media were measured by ELISA. The data were analyzed by repeated measures ANOVA. When GL from F1 follicle cultured alone, GH did not affect progesterone production in YLC hens and decreased it from 30,5±3,4 to 20,5±2,9 ng/mg tissue (P < 0.01) in OSC hens. Conversely, when tested GL from F2 follicle, GH increased progesterone output from 15,8±2,4 to 20,4±2,5 ng/mg tissue (P < 0.05) in YLC birds and had no effect on the output in OSC birds. During co-culture of GL and TL, GH raised 1.4–1.5 times the production of progesterone in the case of F1 follicle and did not change it in the case of F2 follicle in hens of both ages. The findings indicate that the steroidogenic response of GL from the two largest preovulatory follicles to GH differs in young and old hens. However, the interaction with TL modifies the GL response and makes it similar in birds regardless the age and reproductive status. The study was supported by RFBR (19-016-00216).
PSXI-3 Applicability of a non-invasive method to predict body weight of growing-finishing pigs based on the support of the front legs
Authors: López-Vergé Sergi , Aymerich Pau , Soldevila Carme , Bonet Jordi , Gasa Josep , Coma Jaume , Castillejos Lorena
Published: November 2020

The aim of the current study was to verify the applicability of an automatic weighting method based on the support of the front legs of the pigs compared to a traditional scale. A total of 1,320 males and females crossbreed pigs [Pietrain x (Landrace x Large White)] from 110 sows were studied. Feed was offered ad libitum. All pigs were allotted to growing-fattening pens (13 pigs/pen) and individually identified by electronic ear tags. A scale with a platform to put the front legs (FLS), also equipped with an antenna to read the ear tags, was placed in each pen assembled with the feeder, weighing the pig automatically each time approached for feed. All pigs were also manually weighed with a regular scale (RS) on d 64 (entry to the fattening unit), d 90, d 111, d 140 and d 159, respectively, to compare both weighting methods. The relationship between the BW obtained by FLS and RS was analyzed by using the CORR procedure of SAS. Thereafter, a simple linear model was adjusted by using the REG procedure of SAS to predict the BW of the animals from the FLS method. The results showed a high correlation (r = 0.99; P < .0001) between the two weighting methods. Moreover, the linear model fitted (RS = -1.6799 + 1.7413 x FLS; P < .0001) presented a high R2 (0.99), low CV (4.29%) and low RMSE (2.641). Results suggest that the FLS is a good method to weight individually and automatically all the pigs of a growing-fattening unit allowing farmers to monitor the growth of their animals and to implement feeding strategies for pigs that are growing below the average (e.g. precision feeding). The benefits of animal welfare by reducing the stress associated to the over manipulation of pigs should also be considered.
PSII-12 Effect of aspirin to intentionally induce leaky gut on performance, blood markers of stress, and carcass characteristics of feedlot cattle
Authors: Briggs Nathan , Schoonmaker Jon P
Published: November 2020

The negative impacts of stress on gastrointestinal (GIT) barrier function can result in compromised animal health. Aspirin (acetylsalicylic acid) is known to cause mucosal injury leading to increased gut permeability and tight junction damage. The objective of this study was to determine the long-term impact of leaky gut on animal physiology. In this experiment, 96 Simmental x Angus steers (355 ± 14.8 kg) were allotted by body weight and breed into two treatments: control (no aspirin) or aspirin fed at 50 mg/kg/d for 159 d. Steers were housed in 16 pens (8 pens/treatment) with 6 steers in each pen. Weight was recorded monthly and serum was collected on d 159 to be analyzed for lipopolysaccharide binding protein (LBP), interleukin-6 (IL-6), serum amyloid A (SAA), haptoglobin, and aspartate aminotransferase (AST). Data were analyzed using the MIXED procedure of SAS. Average daily gain (ADG) tended to decrease in cattle fed aspirin (P= 0.10). Aspirin decreased hot carcass weight (P= 0.05) and rib-eye area (P= 0.01)and increased fat thickness (P= 0.02), marbling score (P = 0.003), and yield grade (P = 0.01). Percent KPH tended to increase (P= 0.10) for steers fed aspirin. Aspirin had no effect on body weight, dry matter intake, gain:feed, days on feed, dressing percentage, liver abscess score, or percent liver abscesses. Aspirin tended to increase serum LBP (P= 0.07), but had no effect on serum concentrations of IL-6, haptoglobin, SAA, and AST (P≥ 0.30). This study indicates that leaky gut induced by long-term administration of aspirin has negative impacts on feedlot performance and carcass leanness. The negative impact of aspirin induced leaky gut on animal performance suggests that leaky gut caused by other factors (subacute acidosis, stress) may be a significant problem for the feedlot industry.
PSII-22 Effects of Clinoptilolite inclusion on Ruminant Invitro Digestibility
Authors: Lente Leeroy A , Murray Roderick A Gonzalez , Archibeque Shawn L
Published: November 2020

The aim of this study was to assess the effects of Zeolite (ZE, clinoptilolite) inclusion on in vitro rumen fermentation. A modified procedure from Tilley and Terry (1963) was used to determine alfalfa in vitro dry matter digestibility (IVDMD) in the presence or absence of ZE. Test tubes (n = 96) were placed in a 39 °C bath and were blocked based on hours 0, 1, 2, 4, 6, 8, 12, 24, and 48. Substrate consisted of dried alfalfa grinded through a 1mm screen using a Wily Mill. Treatments consisted of, Control (no ZE inclusion), and 0.05g ZE, for each 1 gram of alfalfa. After incubation, IVDMD was calculated 24 hours after drying period. Data was analyzed using a randomized block design. The IVDMD was found to be similar across all treatments (P > 0.8464). The IVDMD was significantly different when incubated for the varying lengths of time (P < 0.001). There was an IVDMD of 20.18 ± 2.89 % for the control and 18.07 ± 5.49 % for the ZE at the zero hour. The IVDMD for Control and ZE for 48 hours were 54.30 ± 1.58 % and 53. 48 ± 1.04 % respectively. To predict the IVDMD of the effects of ZE treatment on digestibility over time, the corresponding regression f(x) = 20.60 + 0.421X1 + 0.730X2 with R2 = 0.8464. In conclusion these data demonstrate that inclusion of ZE, does not influence the in vitro digestibility of alfalfa. These findings are important as previous data would indicate that inclusion of ZE may reduce cost of gain and/or influence liver abscess rates, it was uncertain if that would come at the cost of reduced digestibility of feed. These data indicate that there is likely very little to no impact on feed digestibility when ZE is included in the ration.
PSIX-17 Investigation of rumen starch and protein degradation kinetics in relation to corn processed by super-conditioned pelleting, extruding and puffing
Authors: Rahimi Atieh - , Naserian Abbasali , Valizadeh Reza , Tahmasebi Abdolmansour , Dehghani Hesam , Soltani Esmaeil , Sung Kyung-Il , Kim Byong-Wan , Kim Ji-Yung , Lee Bae-Hun , Nejad Jalil Ghasemi
Published: November 2020

The objective of this study was to investigate the rumen dry matter (DM), starch and protein degradation kinetics of an Iranian corn grain (single crass 702) processed using different thermal processing methods including 1) grinding (G, 2mm), 2) super-conditioned pelleting (SCP; moisture 20%, retention time 6 min and conditioning temperature 95°C), 3) puffing (PUF; puffing temperature 200°C, hot air velocity 25 m/s, feed rate 100 g/m) and 4) extruding (EX; moisture 20%, temperature of melting zone 75°C and die zone 125°C and time 150s). Three lactating Holstein cows fitted with a ruminal cannula were used. Samples were incubated into the rumen for 2, 4, 8, 12, 24, and 48 h. Rumen degradation kinetics were fitted based on equations. Data were analyzed by GLM procedure of SAS (P < 0.05) using a completely randomized design with 6 replications for every treatment. Rapidly soluble fraction (a) and fractional rate constant (c) of DM were higher (P < 0.01) in PUF and EX corns than SCP. The lowest (P < 0.05) slowly degradable fraction (b) of DM was found in SCP. The highest (P < 0.01) effective rumen degradability of DM (ERDM), starch (ERDS) and protein (ERDP) were observed in the EX, followed by PUF, SCP and G corns. Fraction a of starch and protein was significantly greater (P < 0.01) in EX, PUF and SCP than G. The rate of degradability (c) of starch, DM and protein in EX and PUF were similar and higher (P < 0.01) than SCP and G, but c for starch in EX was greater (P < 0.01) than PUF and fallowed by SCP and G corns. In conclusion, the super-conditioning of corn as a new method of corn processing increased rapidly soluble fraction starch similar to PUF and EX corns and effective rumen degradability of starch was significantly increased in SCP corn.
PSIX-14 Influence of living yeast of the genus Rhodotorula against probiotic and pathogenic bacteria
Authors: Nikanova Daria A , Kolodina Evgenia
Published: November 2020

The aim of the research was to study the effect of live yeast Rhodotorula spp. (LYR) on the growth and development of microorganisms and microbial profiles in batch culture. A liquid medium was used to prepare the inoculum (20 g/L glucose, 20 g/L peptone, 10 g/L yeast extract). The study in vitro was a 3×5 factorial arrangement, including low (5.0) and high media pH (7.5) and temperature (from 20 to 39 ° C). The treatments were LYR with concentrations from 1·103 to 1·1011 CFU/ml. The bacteria were selected: Lactobacillus casei subsp. Rhamnosus ATCC 7469, Bifidobacterium breve ATCC 15701, E. coli ATCC 25922, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 14990. The analyses were carried out separately for each culture and LYR concentration by broth dilution. For incubation were used Saburo agar for LYR and differential-diagnostic media for each bacterial species. After incubation, there was a lack of growth of E. coli, S. aureus and S. epidermidis in LYR concentrations from 1·109 to 1·1011 CFU/ml at the temperature of 28±0.5 °С, while the number of yeast cells did not decrease. At the temperature above 32±0.5 °C there was a decrease in the amount of LYR by more than 1000-fold in the samples of all cultures. The presence of LYR in the medium led to an increase (p≤0.001) in the number of L. casei at pH 5.0 and temperature less than 30±0.5 °С. Unlike L. casei, the viability of the B. breve culture has decreased (P ≤0.05) by 200 times at pH 5.0, but has increased (p≤0.001) by more than 1000 times at pH 7.0. The optimal cultivation temperature was 36±0.5 °С. These results indicate the multidirectional effect of LYR on microorganisms in vitro. This work was supported by the Ministry of Science and Higher Education of Russia (topics GZ AAAA-A18-118021590136-7).
PSIX-10 In vitro dry matter digestibility, gas production and pH of silages of several maize hybrids
Authors: Queiroz Pedro , Filho Israel Alves , Junior Sergio Pereira , van Cleef Flavia Scarpino , Ezequiel Jane , Kaneko Flavio , van Cleef Eric H C B
Published: November 2020

The objective of this study was to determine the in vitro dry matter digestibility (IVDMD), gas production and pH of silages of 10 maize hybrids in Iturama, Brazil (XB 6012 BT, BM 709 VT PRO2, BM 815 VT PRO2, DKB 363 VT PRO3, AG 8740 VT PRO3, SYN 555 VIP3, SX 8555 VIP3, SX 7005 VIP3, DKB 390 VT PRO3, and RB 9789 VIP3). The maize was ensiled in experimental mini silos in a completely randomized design with 3 repetitions. After 120 d, a subsample from each mini silo was taken and the IVDMD was estimated using Ankom Daisy II and F-57 bags (n = 44). Rumen fluid from two cannulated Nellore steers were mixed and placed into fermenter jars containing Ankom buffers (1:4; v/v). After 48-h incubation (39ºC), 40 mL 6 N HCl and 8 g pepsin were added to each jar and incubated for another 24 h. For gas production and pH evaluations, penicillin-type glass vials (n = 40) were filled with McDougall buffer (20 mL), rumen fluid (10 mL) and substrates (200 mg) and incubated in a shaker (39ºC) for 24 h. The cumulative pressure was measured, vials were opened, and terminal pH was measured. Data were analyzed using the MIXED procedure of SAS. The DKB 363 VT PRO3 presented the greatest IVDMD (84.9%, P = 0.02) and the lowest pH (6.60, P = 0.002). The opposite was observed for DKB 390 VT PRO3, which presented the lowest IVDMD (71.1%) and the greatest pH (6.65). The BM 709 PRO2 produced more gas than the others (246.1 mL/g DM), while SYN 555 VIP3 produced less gas than the others (167.7 mL/g DM, P = 0.0005). In conclusion, the material DKB 363 VT PRO3 presented the most interesting characteristics for use as silage for beef cattle in Iturama, Brazil.
PSIX-20 Mechanisms of Programmed Nutrition in finishing cattle
Authors: Schiff Amanda P , McLeod Kyle , Klotz James L , Holder Vaughn , Harmon David L
Published: November 2020

Programmed Nutrition Beef Program (Alltech Inc.) is a supplement that could reduce the use of feed additives such as Monensin and Tylosin in feedlots. The objective of this study was to examine changes in rumen fermentation when feeding Monensin/Tylosin and Programmed Nutrition. Eight steers (BW = 363 ± 22 kg) were used in a replicated 4 x 4 Latin square design experiment in a 2 x 2 factorial treatment structure where animals were fed a high-concentrate diet at 2.0 x NEm. Treatments were Control (C; conventional trace mineral supplement), Control + Monensin + Tylosin (MT), Programmed Nutrition (PN; Programmed Nutrition Beef Finisher), or Programmed Nutrition + Monensin + Tylosin (PNMT), incorporated daily to the diet at 75 g/day. Rumen pH was measured continuously for 48 h, rumen fluid was collected every 2 h for 48 h and analyzed for VFA. Dietary digestibility, nitrogen and energy balance were determined by collection of total urine and fecal output for 7 days, and indirect calorimetry for 48 h. Protein turnover was determined via 15N-glycine end-product method. The experiment was analyzed as a replicated Latin square design with mixed models of SAS (SAS Inst. Inc.). Mean differences were analyzed using LSD, rumen VFA and pH data were analyzed using repeated measures. There were no differences between treatments in DMI, ADG, dry matter digestibility and rumen pH. Addition of MT to the diet lowered fecal N output (P = 0.0092), ruminal valerate concentration (P = 0.0125) and molar proportion (P = 0.0235). When PN was fed in combination with MT it decreased ADF digestibility (P = 0.0342), protein turnover (P = 0.0211), protein synthesis (P = 0.0362) and protein degradation (P = 0.0292). Although no differences in ADG and dry matter digestibility were observed, significant metabolic changes occurred when supplementing PN with or without MT.