bluuebunny/crossref_metadata_2025 huggingface dataset:
Could the Anatomic Variants of the Superior Thalamic Vein (STV) Be Considered a Possible Landmark for Target Identification in Magnetic-Resonance-Guided Focused Ultrasound Procedures? A Pilot Study Using Susceptibility Weighted Imaging Sequences
Authors: Cammaroto Simona , Acri Giuseppe , Hartwig Valentina , Morabito Rosa , Militi Annalisa , Smorto Chiara , Ielo Augusto , Bonanno Lilla , Anfuso Carmelo , Quartarone Angelo
Published: July 2024

During magnetic-resonance-guided focused ultrasound ablation of the ventral intermediate thalamic nucleus (VIM) for essential tremor (ET) and Parkinson’s disease (PD), targeting is generally performed using a standard atlas-based stereotactic approach. The purpose of our work is to evaluate the anatomic variations in the venous vasculature of the thalamus in patients treated with MRgFUS, as a possible landmark for targeting. We retrospectively evaluated the relationship between the obtained thalamotomy lesion and the ipsilateral superior thalamic vein (STV). A total of 36 patients (25 ET and 11 PD) who underwent MRgFUS treatment were evaluated, and the STV was studied with susceptibility weighted imaging (SWI) sequences. Based on the axial SWI images, the distance between the STV and the center of the lesion at the presumed site of the VIM was measured in follow-up MRI images one month after treatment. Statistical analysis shows that there is a correlation between the STV and the presumed site of the VIM. The STV visible in SWI could be used as an additional, real-time, and patient-specific anatomical landmark for VIM identification during MR examination and just before and during FUS treatment.
SARS-CoV-2 Infection during Delivery Causes Histopathological Changes in the Placenta
Authors: Borowczak Jędrzej , Gąsiorek-Kwiatkowska Agnieszka , Szczerbowski Krzysztof , Maniewski Mateusz , Zdrenka Marek , Szadurska-Noga Marta , Gostomczyk Karol , Rutkiewicz Paula , Olejnik Katarzyna , Cnota Wojciech , Karpów-Greiner Magdalena , Knypiński Wojciech , Sekielska-Domanowska Marta , Ludwikowski Grzegorz , Dubiel Mariusz , Szylberg Łukasz , Bodnar Magdalena
Published: July 2024

Background: SARS-CoV-2 can damage human placentas, leading to pregnancy complications, such as preeclampsia and premature birth. This study investigates the histopathological changes found in COVID-19-affected placentas. Materials and Methods: This study included 23 placentas from patients with active COVID-19 during delivery and 22 samples from patients without COVID-19 infection in their medical history. The samples underwent histopathological examination for pathology, such as trophoblast necrosis, signs of vessel damage, or fetal vascular malperfusion. Results: Newborns from the research group have lower weights and Apgar scores than healthy newborns. In the COVID-19 group, calcifications and collapsed intervillous space were more frequent, and inflammation was more severe than in the healthy group. At the same time, the placenta of SARS-CoV-2-positive patients showed signs of accelerated vascular maturation. Trophoblast necrosis was found only in the placentas of the research group. The expression of CD68+ was elevated in the COVID-19 cohort, suggesting that macrophages constituted a significant part of the inflammatory infiltrate. The increase in lymphocyte B markers was associated with placental infarctions, while high levels of CD3+, specific for cytotoxic T lymphocytes, correlated with vascular injury. Conclusions: SARS-CoV-2 is associated with pathological changes in the placenta, including trophoblast necrosis, calcification, and accelerated villous maturation. Those changes appear to be driven by T cells and macrophages, whose increased expression reflects ongoing histiocytic intervillositis in the placenta.
Entanglement Entropy of Free Fermions with a Random Matrix as a One-Body Hamiltonian
Authors: Pastur Leonid , Slavin Victor
Published: July 2024

We consider a quantum system of large size N and its subsystem of size L, assuming that N is much larger than L, which can also be sufficiently large, i.e., 1≪L≲N. A widely accepted mathematical version of this inequality is the asymptotic regime of successive limits: first the macroscopic limit N→∞, then an asymptotic analysis of the entanglement entropy as L→∞. In this paper, we consider another version of the above inequality: the regime of asymptotically proportional L and N, i.e., the simultaneous limits L→∞,N→∞,L/N→λ>0. Specifically, we consider a system of free fermions that is in its ground state, and such that its one-body Hamiltonian is a large random matrix, which is often used to model long-range hopping. By using random matrix theory, we show that in this case, the entanglement entropy obeys the volume law known for systems with short-range hopping but described either by a mixed state or a pure strongly excited state of the Hamiltonian. We also give streamlined proof of Page’s formula for the entanglement entropy of black hole radiation for a wide class of typical ground states, thereby proving the universality and the typicality of the formula.
Energy, Exergetic, and Thermoeconomic Analyses of Hydrogen-Fueled 1-kW Proton-Exchange Membrane Fuel Cell
Authors: Yoo Yungpil , Lee Sang-Yup , Seo Seok-Ho , Oh Si-Doek , Kwak Ho-Young
Published: July 2024

Exergy analysis evaluates the efficiency of system components by quantifying the rate of entropy generation. In general, the exergy destruction rate or irreversibility rate was directly obtained through the exergy balance equation. However, this method cannot determine the origin of the component’s entropy generation rate, which is a very important factor in system design and improvement. In this study, a thorough energy, exergy, and thermoeconomic analysis of a proton-exchange membrane fuel cell (PEMFC) was performed, providing the heat transfer rate, entropy generation rate, and cost loss rate of each component. The irreversibility rate of each component was obtained by the Gouy–Stodola theorem. Detailed and extensive exergy and thermoeconomic analyses of the PEMFC system determined that water cooling units experience the greatest heat transfer among the components in the studied PEMFC system, resulting in the greatest irreversibility and, thus, the greatest monetary flow loss.
Teacher Education: Design Thinking Approach in Makerspaces to Produce Quality Educational Video Games with a Visual Identity and Improve Design Thinking Skills
Authors: Zaky Yara Ahmed Mohebeldin , Al Mulhim Ensaf Nasser
Published: July 2024

Video games participate effectively in the educational process due to their attractive visual features, but there are many challenges that teachers face when using them. Among these challenges is designing games to suit the goals of the educational process and respecting societal identity. Although there are many studies on educational video games, limited approaches that enhance teachers’ education have been explored. The current study focuses on stimulating pre-service teachers’ design thinking skills and improving the quality of their video games that have a visual identity by incorporating a design thinking approach into a makerspace. This study followed a quasi-experimental approach, in which 38 pre-service teachers from the departments of Art Education, Early Childhood Education, and Educational Technology at the College of Education at King Faisal University in Saudi Arabia participated. They designed and produced educational video games that consider visual identity and completed the design thinking scale. The results showed that there was great cooperation between participants from all disciplines in the work team, which affected the generation of creative and innovative ideas and the quality of the educational video games. This is due to the use of design thinking elements such as empathy, identification, ideation, and prototyping with different tools in the makerspace. This study calls for the use of a design thinking approach in the classroom learning space for teachers’ education, discusses implications for educational practices, and recommends further research in this area.
Claiming Space to (Re)generate: The Impact of Critical Race Professional Development on Teacher Educators of Color
Authors: Leathers Sharon , Nevárez Diane Mendoza , Arteaga Nallely , Kohli Rita , Pizarro Marcos
Published: July 2024

As educational systems are confronted with attacks under the guise of “Critical Race Theory” bans, teacher educators of Color navigate the contradictions of preparing teacher candidates to be culturally sustaining within a suppression of racial discourses. For many teacher educators of Color, who are often tasked to carry out the social and racial justice work of teacher education programs, they are experiencing an exacerbated racial harm. In this article, we explore how a racial-affinity critical race professional development (CRPD) space for teacher educators of Color committed to racial justice serves as a space of support, healing, and regeneration amidst systemic racism and protections to white comfort in teacher education. Weaving together three counterstories from participants in the CRPD, we examine how this space supports teacher educators in recentering communities of Color knowledge systems and ways of being to sustain themselves and reclaim teacher education. These counterstories also offer implications for teacher education to address the ways in which it supports and maintains white comfort and the need for a restorative framework for addressing past and ongoing racial harm.
Stable Operation Domain Analysis of Back-to-Back Converter Based Flexible Multi-State Switches Considering PLL Dynamics
Authors: Wang Xunting , Ding Jinjin , Xu Bin , Meng Xiaoxiao , Qi Zhengyi
Published: July 2024

Flexible multi-state switches (FMSSs), also called soft open point (SOP), are regarded as the core devices in flexible interconnected distribution network. However, due to their more complex control structure and nonlinear characteristics, there is a tight and complex coupling relationship between the transmission power limit and stability of two (or more) converter sides, which brings difficulties to the planning and power flow dispatching control of flexible interconnected distribution networks. In this paper, taking the back-to-back converter based FMSS (BTBC-FMSS) as the research object, the dynamic equivalent circuit connected to the distribution network is constructed, and the transmission active and reactive power expression of the converter on both sides considering PLL dynamics is deduced. The variation rules of transmission power limits on both sides of the device with various structures and control parameters are summarized. Furthermore, the stable operation domain of the BTBC-FMSS is constructed, and the effective measures to improve the transmission power stability of the device are given according to the analysis of the stable operation domain. Finally, a simulation model is built in Matlab/Simulink to verify the effectiveness of the analysis results.
Improving Balance and Functional Status in Spinal Cord Injury Patients: A Systematic Review Comparing Virtual Reality-Based Therapy and Conventional Therapeutic Exercises
Authors: Obrero-Gaitán Esteban , López-Nájera Luis , Piñar-Lara Marina , Ibancos-Losada María del Rocío , Cortés-Pérez Irene , García-López Héctor
Published: July 2024

Background: The objective was to evaluate the efficacy of virtual reality-based therapy (VRBT) compared to therapeutic exercise in increasing balance and functional status in patients with acquired spinal cord injury (SCI). Methods: Following PRISMA recommendations, we searched randomized controlled trials that meets the inclusion criteria in PubMed Medline, Scopus, Web of Science and PEDro without language and publication date limitations. Methodological quality and risk of bias were evaluated using the PEDro scale. Results: Six RCTs providing data on 131 patients with SCI were included in this systematic review. Based on the information gathered from the included studies, both therapies can increase balance and functional status between pre- and post-assessment. However, it seems that there is a higher rate of improvement in balance and function in SCI patients in favor of the group that uses VRBT in their treatment (p < 0.05). Conclusions: Although there is evidence in favor of the effectiveness of VRBT in increasing balance and functional status in patients with SCI, this is limited, and further research is necessary. Even so, taking into account the results found, our systematic review suggests that VRBT provides an effective therapeutic use to implement alongside conventional therapies for patients with SCI.
Convection Heat-Transfer Characteristics of Supercritical Pressure RP-3 in Horizontal Microchannels
Authors: Zhang Qiaoling , Wang Kangming , Yu Ziyuan , Ma Haoran , Huang Biyun
Published: July 2024

To enhance the heat-transfer performance of scramjet engines, a numerical simulation was conducted on the heat-transfer process of RP-3 aviation kerosene under supercritical pressure within a horizontal micro-fine circular tube. The intrinsic mechanism of the heat-transfer process was analyzed, summarizing the impacts of mass flux, inlet temperature, and gravitational acceleration. Furthermore, four commonly used buoyancy criterion numbers were compared and evaluated. The results indicate that the heat-transfer process can be divided into five phases: heating inlet phase, normal heat-transfer phase, heat-transfer deterioration phase, heat-transfer enhancement phase, and high-temperature normal heat-transfer phase. The heating inlet phase is significantly influenced by the inlet temperature, while the heat-transfer deterioration is affected both by the thermal property variations of the aviation kerosene and the buoyancy effects. Lower mass flux and hypergravity conditions all exacerbate heat-transfer deterioration. Inlet temperature, however, does not affect the heat-transfer pattern. Among the criteria, Grq/Grth provides the best prediction of buoyancy effects in horizontal circular tubes.
Thermal and Hydrodynamic Measurements of a Novel Chaotic Micromixer to Enhance Mixing Performance
Authors: Mahammedi Abdelkader , Kouider Rahmani , Tayeb Naas Toufik , Kassir Al-Karany Raúl , Cuerda-Correa Eduardo M. , Al-Kassir Awf
Published: July 2024

In this study, three-dimensional simulations were conducted on a new passive micromixer to assess the thermal and hydrodynamic behaviors of Newtonian and non-Newtonian fluids subjected to low generalized Reynolds numbers (0.1 to 50) and shear-thinning properties. To acquire a more profound comprehension of the qualitative and quantitative fluctuations in fluid fraction using the CFD Fluent Code, the mass mixing index, rheological behavior, performance index, mixing energy cost, mass fraction distributions, temperature contours, and pressure drop were compared to illustrate the importance of the mixer geometry in the context of two miscible fluids with varying inlet temperatures. The selected geometry is characterized by a robust chaotic flow that substantially enhances thermal and hydrodynamic performance across all Reynolds numbers. A mass mixing exceeding 72.5% is obtained when Re = 5, reaching 93.5% when Re = 50. Furthermore, the evolution of thermal mixing for all behavior indexes reaches a step of 98% with minimal pressure losses. This work enabled the demonstration of a chaotic geometry in a highly efficient mixing system, leading to enhanced thermal performance for both Newtonian and non-Newtonian fluids. The results of the hydrodynamic and thermal characterization of the mixing of shear-thinning fluids within the micromixers under investigation are conclusive.
Evaluation of Life Cycle CO2 Emissions for the LDR-50 Nuclear District Heating Reactor
Authors: Sokka Laura , Kirppu Heidi , Leppänen Jaakko
Published: July 2024

The LDR-50 low-temperature nuclear reactor is designed for the Finnish and European district heating markets, as an environmentally sustainable heating option for the 2030s. While the carbon footprint of conventional electricity-producing reactors is known to be small, there have been no comprehensive studies on the emission reduction potential when the technology is applied to the heating sector. This paper aims to fill this knowledge gap by means of life cycle assessment (LCA) analysis. The carbon footprint of the LDR-50 heating plant is evaluated, and compared to conventional heating fuels, direct electric heating, and heat pumps. The results of the analysis show that the life cycle CO2 emissions are low, although there are still significant uncertainties related to the construction phase, due to missing data. In addition to carbon footprint, the analysis is also extended to other adverse environmental impacts. It is concluded that significant reductions in CO2 emissions can be achieved by replacing fossil heating fuels with nuclear energy. The technology is considered a viable option alongside biofuels and heat pumps. The overall environmental impacts are low, and the production does not compete for low-carbon electricity or scarce natural resources.
Seed Availability and Small Mammal Populations: Insights from Mediterranean Forests
Authors: Llanos-Guerrero César , Freixas-Mora Lídia , Vilella Marc , Bartrina Carme , Torre Ignasi
Published: July 2024

Plant–animal interactions play a crucial role in ecosystem functioning, especially through seed dispersal mechanisms. Understanding how small mammal populations respond to seed availability is essential for ecosystem management and biodiversity conservation, especially in the context of habitat loss and climate change. We conducted a 10-year study in mixed Mediterranean oak–beech forests to investigate the population dynamics of common small mammal species in response to seed availability. Our findings revealed distinct responses among species, influenced by life history traits, foraging behaviour, and diet. Wood mice (Apodemus sylvaticus) showed a rapid population increase with seed availability both in the same year of seed fall and the following year, suggesting a flexible foraging strategy and a dependence on arboreal seed producers. Yellow-necked mice (Apodemus flavicollis) revealed immediate population increases in response to seed availability in the autumn, probably because of their arboreal habits and preference for exploiting seeds prior to maturation. Bank voles (Clethrionomys glareolus) showed responses with population peaks in years following high seed availability, indicating a slower demographic response to resource abundance. Surprisingly, the greater white-toothed shrew (Crocidura russula) responded indirectly to seed availability in Mediterranean forests, suggesting complex interactions with seed-associated invertebrates or dependence on other variables not considered. Our findings highlight the importance of understanding how changes in seed availability influence the population ecology of small mammals, with significant implications for the conservation and management of Mediterranean forest ecosystems in the context of climate change and recurrent droughts. These results emphasise the need to consider species interactions, resource availability, and climate change in the conservation and management of evolving ecosystems.
Effects of Neolamarckia cadamba Leaf Extract on Dynamic Fermentation Characteristics and Bacterial Community of Stylosanthes guianensis Silage
Authors: Huang Peishan , Chen Mengmeng , Chen Dekui , Zang Meiqi , Zhang Weiling , Lin Xiyue , Han Hongyan , Zhang Qing
Published: July 2024

This study focused on exploring the effects of Neolamarckia cadamba leaf extract (NE) on the fermentation process and bacterial community of stylo (Stylosanthes guianensis) silage. Fresh raw materials of stylo were ensiled with 0%, 1%, and 2% NE, and various fermentation parameters, nitrogen components, and microbial compositions were analyzed at different time points (days 3, 7, 14, and 30) during the ensiling process. The experiment showed that, in comparison to the control group, incorporating NE into the ensiling process resulted in improved fermentation parameters, including increased lactic acid and acetic acid levels, as well as decreased pH, coliform population, and ammonia nitrogen concentration. Moreover, the relative abundances of Lactobacillus and Pediococcus were augmented, while the growth of Enterobacter was inhibited by the NE addition. These results suggest that NE has potential as a novel additive for silage, promoting a reduction in harmful bacteria and enhancements in the nutritional quality and fermentation characteristics of stylo silage.
Study of the Safety Characteristics of Different Types of Pepper Powder (Capsicum L.)
Authors: Kosár László , Szabová Zuzana , Kuracina Richard , Spitzer Stefan H. , Mynarz Miroslav , Filipi Bohdan
Published: July 2024

This research was aimed at comparing the fire characteristics of different types of pepper in the context of explosion prevention. The following characteristics were studied: explosion pressure Pmax and Kst at selected concentrations, ignition temperature of the deposited dust layer from the hot surface, and minimum ignition energy. The comparison of the chemical properties of the used types of pepper was performed using TG/DSC. The results of the measurements suggest that different types of peppers exhibit different explosion characteristics. Each sample reached the maximum value of the explosion pressure and rate of pressure rise at different concentrations. The volume of the explosion chamber used also influenced the explosion characteristics. It is a consequence of the fact that the explosion characteristics strongly depend on the mechanism of action of a particular igniter. The minimum effect on the safety characteristics was observed when measuring the minimum ignition energy and the minimum ignition temperature of the dust layer from the hot surface. The results of the measurements suggest that different types of peppers exhibit different explosion characteristics. This information should then be considered in explosion prevention.
Integrated Rice–Fish Culture Alters the Bacterioplankton Community and Its Assembly Processes
Authors: Diao Weixu , Yuan Julin , Jia Rui , Hou Yiran , Zhang Liqiang , Li Bing , Zhu Jian
Published: July 2024

The rice–fish co-culture is an important model of carp farming in China; however, research on the dynamics and assembly of water bacterial communities in this system remains limited. Therefore, this study aimed to explore the dynamics and assembly of bacterial communities, as well as their correlation with environmental factors in paddy water. For these purposes, this study was divided into two groups: a rice–carp co-culture (WRC) group and a rice monoculture (WRM) group, with 20 rice fields in each group. After 60 days of farming, the concentrations of NH4+-N, NO2−-N, TN, and PO43− were significantly higher in the WRC group than those in the WRM group. Alpha diversity analysis showed that the Shannon index in the WRC group was significantly decreased compared with the WRM group. At the phylum level, the relative abundance of Actinobacteria significantly increased, while the relative abundance of Proteobacteria and Bacteroidetes significantly decreased in the paddy water of the WRC group. The neutral community model (NCM) indicated that a random process played a dominant role in the construction of bacterial communities in the two groups, and common carp cultivation increased migration rates, thereby affecting community assembly. The co-occurrence network displayed that common carp cultivation led to looser interactions between bacterial communities. In addition, the contents of nutrients significantly affected the abundance of bacteria in paddy water. In summary, carp cultivation decreased the diversity of bacteria and changed the relative abundance of dominant bacteria, thereby affecting the stability and assembly of bacterial communities.
Population Dynamics Parameters and Exploitation Status of 55 Commercial Species in Egyptian Red Sea Fisheries: A Key to Sustainable Fisheries
Authors: Mehanna Sahar F. , Samy-Kamal Mohamed
Published: July 2024

Egyptian Red Sea fisheries face the same challenges as most of the world’s fisheries, including overexploitation, habitat loss, IUU fishing, pollution, and climate change. These fisheries are highly diverse with multiple species targeted by multiple fleets, using different fishing gears. Much work has been performed in recent years to assess Red Sea fish stocks. However, not all fish stocks in the Egyptian Red Sea are assessed, and those that are assessed only cover 30% of landings. The assessments are unbalanced by area, with the Gulf of Suez being much better covered than the southern Red Sea and Gulf of Aqaba. The results show that most of the analyzed stocks are overexploited. There is an urgent need to take action to protect, conserve, and restore the different fish stocks in different fishing grounds. These actions will ensure the sustainability of the fisheries, making them ecologically friendly and economically and socially efficient.
Experimental Investigation of the Performance of a Novel Ejector–Diffuser System with Different Supersonic Nozzle Arrays
Authors: Xu Dachuan , Gu Yunsong , Li Wei , Chen Jingxiang
Published: July 2024

The supersonic–supersonic ejector–diffuser system is employed to suck supersonic low-pressure and low-temperature flow into a high-pressure environment. A new design of a supersonic–supersonic ejector–diffuser was introduced to verify pressure control performance under different operating conditions and vacuum background pressure. A 1D analysis was used to predict the geometrical structure of an ejector–diffuser with a rectangular section based on the given operating conditions. Different numbers and types of nozzle plates were designed and installed on the ejector to study the realizability of avoiding or postponing the aerodynamic choking phenomenon in the mixing section. The effects of different geometrical parameters on the operating performance of the ejector–diffuser system were discussed in detail. Experimental investigation of the effects of different types of nozzle plates and the back pressures on the pressure control performance of the designed ejector–diffuser system were performed in a straight-flow wind tunnel. The results showed that the position, type and number of the nozzle plates have a significant impact on the beginning of the formation of aerodynamic choking. The geometry of the ejector and the operating conditions, especially the backpressure and inlet pressure of the ejecting stream, determined the entrainment ratio of the two supersonic streams. The experimental results showed that long nozzle-plate had a better performance in terms of maintaining pressure stability in the test section, while short a nozzle-plate had a better pressure matching performance and could maintain a higher entrainment ratio under high backpressure conditions.
Evolution of Quality Parameters and Bioactivity of Actinidia chinensis cv. Sungold (Kiwifruit) Slices Subjected to Different Drying Conditions Storage for 4 Months
Authors: Vincenzo Sicari , Antonio Mincione , Rosa Romeo , Roberta Pino , Filomena Conforti , Rosa Loizzo Monica
Published: July 2024

The present study aimed to investigate the impact on nutritional and functional properties of dried kiwifruit (Actinidia chinensis cv. Sungold) slices during conservation for 120 days in sealed containers in the dark at 25 °C. For this purpose, kiwifruits slices were dried at two different temperatures, 40 and 55 °C, for 30 and 25 h, respectively. Fresh and dried kiwi slices were analyzed for their pH, activity water, total solid soluble (TSS), color, titratable acidity, total phenols (TPC) and flavonoids content (TFC), organic acids, and radical scavenging activities. Analysis carried out on the dehydrated samples showed a good aptitude of kiwi material towards the drying process. Particularly, it has been observed that the drying treatment at low temperature helped to preserve the nutraceutical properties of the fruits. In fact, samples treated at 40 °C (KLT) showed at day 0 (T0) the highest TPC and TFC with values of 979.42 Gallic Acid Equivalents (GAE)/100 g of dried weight (dw) and 281.84 mg catechin equivalents (CTE)/100 g dw even if compared with fresh kiwi slices sample (FKF). Moreover, KLT also exhibited the highest values of antioxidant activity (1657 mmol Trolox/100 g dw). After 120 days storage, all dried samples showed a high ascorbic acid content (429–339 mg/100 g dw fruits) and only a slight variation of physicochemical parameters. Textural Parameters (hardness, springiness, cohesiveness, gumminess, and chewiness), apart from resilience results, showed significant differences between kiwifruit dried at 55 °C and at 50 °C (KLT and KHT, respectively). Color and aroma intensity were the main sensory descriptors with higher scores.
Effect of Tea Tree Essential Oil on the Quality, Antioxidant Activity, and Microbiological Safety of Lightly Processed Lily (Lilium brownii var. viridulum) during Storage
Authors: Guan Yuge , Lu Sainan , Sun Yan , Zhang Rentao , Lu Xinghua , Pang Linjiang , Wang Lei
Published: July 2024

The Lanzhou lily is a regionally distinctive vegetable; the emergence of lightly processed lilies has addressed the inconvenience of consuming fresh lilies. However, the cleaning and impurity removal during the processing of lightly processed lily may strip off its original protective barrier and affect the edible quality. As one of the preservation methods, tea tree essential oil (TTEO) has the characteristics of being green, safe, and efficient preservative properties. This study focused on investigating the effects of different concentrations (25 μL/L, 50 μL/L, and 100 μL/L) of TTEO on the quality and microbiological safety of lightly processed lily. The results showed that compared with the control, appropriate concentrations of TTEO treatment could delay weight loss, improve appearance, firmness, and sensory quality, and maintain microbiological safety with the best effect observed at 50 μL/L. Meanwhile, TTEO treatment induced phenylalanine ammonia-lyase activity, thereby increasing the total phenolic content. Furthermore, TTEO enhanced the superoxide dismutase (SOD) and ascorbate peroxidase (APX) activity, which reduced O2-· production rate and H2O2 content. TTEO inhibited lipoxygenase (LOX) activity, reducing the relative conductivity and malondialdehyde content, thereby delaying lipid peroxidation and quality deterioration. This indicates that TTEO could enhance antioxidant capacity by regulating reactive oxygen species (ROS) metabolism and delay the quality deterioration of lightly processed lily by inhibiting lipid peroxidation.
Development of Hybrid Electrospun Nanofibers: Improving Effects of Cellulose Nanofibers (CNFs) on Electrospinnability of Gelatin
Authors: Hajieghrary Farnaz , Ghanbarzadeh Babak , Pezeshki Akram , Dadashi Saeed , Falcone Pasquale M.
Published: July 2024

Cellulose nanofibers (CNFs) were used to improve the electrospinnability of the gelatin protein in a water/ethanol/acetic acid (3:2:3, v/v) solution. The effects of different concentrations of CNFs (0.5–4%) on the important physical properties of the gelatin solution (15%), including rheology, conductivity, and surface tension, were investigated. The apparent viscosity and shear-thinning behavior were increased by increasing the CNF concentration from 0 to 4% at a low shear rate (<10 s−1). CNFs also increased the electrical conductivity and surface tension of the gelatin solution. Scanning electron microscopy (SEM) images revealed uniformly ordered structures with good continuity without fracture or bead formation in all hybrid nanofibers. They also showed that the average diameters of fibers decreased from 216 nm in the pure gelatin nanofibers to 175.39 nm in the hybrid gelatin/CNF (4%) ones. Differential scanning calorimetry (DSC) results showed that CNFs increased Tg, and X-ray diffraction (XRD) analysis showed that the electrospinning process caused the formation of more amorphous structures in the gelatin/CNF hybrid nanofibers. The tensile test indicated that by adding 2% CNFs, the ultimate tensile strength (UTS) and strain at break (SB) of nanofiber mats increased from 4.26 to 10.5 MPa and 3.3% to 6.25%, respectively. The current study indicated that incorporating CNFs at the optimal concentration into a gelatin solution can improve the resulting hybrid nanofibers’ morphology, average diameter, and mechanical properties.
Sausage Quality during Storage under the Partial Substitution of Meat with Fermented Oyster Mushrooms
Authors: Boylu Meltem , Hitka Géza , Kenesei György
Published: July 2024

The increasing global demand for meat production, driven by a rapidly expanding population and changing dietary preferences has prompted the search for protein-rich, sustainable, and healthier meat alternatives. In this context, edible mushrooms are viewed as advantageous substitutes for meat, offering a viable solution. This study aimed to investigate the effects of partially replacing (25% and 50%) pork meat in sausage samples with fermented oyster mushrooms (Pleurotus ostreatus), which were subjected to various pretreatments. Six different pretreatments were applied to fresh oyster mushrooms as follows: blanching in water, steaming, oven-cooking, microwave treatment, high hydrostatic pressure treatment, and ultraviolet light treatment. The effects of mushroom replacement on the moisture, pH, lipid oxidation, color, and textural properties of sausages during the 4-week refrigerated storage period were evaluated. The results revealed that replacing pork meat with fermented oyster mushrooms resulted in an increase in moisture content and b* values and a decrease in pH, L*, a*, and shear force values, proportional to the mushroom percentage. The lipid oxidation findings suggest that the antioxidant capabilities of fermented oyster mushrooms were influenced by the pretreatment methods applied to the mushrooms, exhibiting varying levels of effectiveness.
Analytical Scheme for Time Fractional Kawahara and Modified Kawahara Problems in Shallow Water Waves
Authors: Nadeem Muhammad , Khan Asad , Javeed Muhammad Awais , Yubin Zhong
Published: July 2024

The Kawahara equation exhibits signal dispersion across lines of transmission and the production of unstable waves from the water in the broad wavelength area. This article explores the computational analysis for the approximate series of time fractional Kawahara (TFK) and modified Kawahara (TFMK) problems. We utilize the Shehu homotopy transform method (SHTM), which combines the Shehu transform (ST) with the homotopy perturbation method (HPM). He’s polynomials using HPM effectively handle the nonlinear terms. The derivatives of fractional order are examined in the Caputo sense. The suggested methodology remains unaffected by any assumptions, restrictions, or hypotheses on variables that could potentially pervert the fractional problem. We present numerical findings via visual representations to indicate the usability and performance of fractional order derivatives for depicting water waves in long-wavelength regions. The significance of our proposed scheme is demonstrated by the consistency of analytical results that align with the exact solutions. These derived results demonstrate that SHTM is an effective and powerful scheme for examining the results in the representation of series for time-fractional problems.
The Future of Total Elbow Arthroplasty: A Statistical Forecast Model for Germany
Authors: Krane Felix , Heck Vincent Johann , Leyendecker Jannik , Klug Kristina , Klug Alexander , Hackl Michael , Kircher Jörn , Müller Lars Peter , Leschinger Tim
Published: July 2024

This study provides a statistical forecast for the development of total elbow arthroplasties (TEAs) in Germany until 2045. The authors used an autoregressive integrated moving average (ARIMA), Error-Trend-Seasonality (ETS), and Poisson model to forecast trends in total elbow arthroplasty based on demographic information and official procedure statistics. They predict a significant increase in total elbow joint replacements, with a higher prevalence among women than men. Comprehensive national data provided by the Federal Statistical Office of Germany (Statistisches Bundesamt) were used to quantify TEA’s total number and incidence rates. Poisson regression, exponential smoothing with Error-Trend-Seasonality, and autoregressive integrated moving average models (ARIMA) were used to predict developments in the total number of surgeries until 2045. Overall, the number of TEAs is projected to increase continuously from 2021 to 2045. This will result in a total number of 982 (TEAs) in 2045 of mostly elderly patients above 80 years. Notably, female patients will receive TEAs 7.5 times more often than men. This is likely influenced by demographic and societal factors such as an ageing population, changes in healthcare access and utilization, and advancements in medical technology. Our projection emphasises the necessity for continuous improvements in surgical training, implant development, and rehabilitation protocols.
Short-Term Liquid Nitrogen Storage of Pyrostegia venusta Embryos: Effects on Germination, Phenotypic and Biochemical Characteristics, and In Vitro Secondary Metabolite Production
Authors: Coimbra Mairon César , Garcia Israel José Pereira , Santos Hérica de Lima , Castro Ana Hortência Fonsêca
Published: July 2024

In this study, short-term liquid nitrogen (LN) storage was used as a strategy to conserve Pyrostegia venusta embryos, and its effects on in vitro germination, phenotypic and biochemical characteristics, and in vitro secondary metabolite production were assessed. Embryos stored in LN for 1 and 7 days presented a higher germination rate and germination speed index compared to those of the control (non-cryostored embryos). Short-term LN storage also favored the phenotypic characteristics of seedlings. LN storage significantly affected the proteins (PTN), soluble sugar (SS) and reducing sugar (RS) contents, oxidative metabolism, and phenylalanine ammonia-lyase (PAL) activity, as well as the total phenolic compound, flavonoid, phytosterol, and alkaloid levels in seedlings regenerated from embryos cryostored for 7 days. Benzoic acid derivatives and flavonoids were observed in regenerated non-acclimatized seedlings. LN storage did not affect the survival rate or phenotypic characteristics of seedlings during acclimatization. Acclimatization promoted significant changes in PTN, SS and RS contents, oxidative metabolism, and PAL activity in seedlings from embryos cryostored for 7 days. Roots from acclimatized seedlings exhibited the highest phenolic, phytosterol, and total alkaloid levels. Differences in the chromatographic profiles of the acclimatized seedlings compared with the non-acclimatized seedlings were observed. LN storage can be an effective means of ex situ conservation of P. venusta genetic resources.
Assessing the Impact of Land Use and Land Cover Changes on Surface Temperature Dynamics Using Google Earth Engine: A Case Study of Tlemcen Municipality, Northwestern Algeria (1989–2019)
Authors: Selka Imene , Mokhtari Abderahemane , Tabet Aoul Kheira , Bengusmia Djamal , KACEMI Malika , Djebbar Khadidja
Published: July 2024

Changes in land use and land cover (LULC) have a significant impact on urban planning and environmental dynamics, especially in regions experiencing rapid urbanization. In this context, by leveraging the Google Earth Engine (GEE), this study evaluates the effects of land use and land cover modifications on surface temperature in a semi-arid zone of northwestern Algeria between 1989 and 2019. Through the analysis of Landsat images on GEE, indices such as normalized difference vegetation index (NDVI), normalized difference built-up index (NDBI), and normalized difference latent heat index (NDLI) were extracted, and the random forest and split window algorithms were used for supervised classification and surface temperature estimation. The multi-index approach combining the Normalized Difference Tillage Index (NDTI), NDBI, and NDVI resulted in kappa coefficients ranging from 0.96 to 0.98. The spatial and temporal analysis of surface temperature revealed an increase of 4 to 6 degrees across the four classes (urban, barren land, vegetation, and forest). The Google Earth Engine approach facilitated detailed spatial and temporal analysis, aiding in understanding surface temperature evolution at various scales. This ability to conduct large-scale and long-term analysis is essential for understanding trends and impacts of land use changes at regional and global levels.
TXNDC5 Plays a Crucial Role in Regulating Endoplasmic Reticulum Activity through Different ER Stress Signaling Pathways in Hepatic Cells
Authors: Bidooki Seyed Hesamoddin , Barranquero Cristina , Sánchez-Marco Javier , Martínez-Beamonte Roberto , Rodríguez-Yoldi María J. , Navarro María A. , Fernandes Susana C. M. , Osada Jesús
Published: July 2024

The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is influenced by a number of variables, including endoplasmic reticulum stress (ER). Thioredoxin domain-containing 5 (TXNDC5) is a member of the protein disulfide isomerase family and acts as an endoplasmic reticulum (ER) chaperone. Nevertheless, the function of TXNDC5 in hepatocytes under ER stress remains largely uncharacterized. In order to identify the role of TXNDC5 in hepatic wild-type (WT) and TXNDC5-deficient (KO) AML12 cell lines, tunicamycin, palmitic acid, and thapsigargin were employed as stressors. Cell viability, mRNA, protein levels, and mRNA splicing were then assayed. The protein expression results of prominent ER stress markers indicated that the ERN1 and EIF2AK3 proteins were downregulated, while the HSPA5 protein was upregulated. Furthermore, the ATF6 protein demonstrated no significant alterations in the absence of TXNDC5 at the protein level. The knockout of TXNDC5 has been demonstrated to increase cellular ROS production and its activity is required to maintain normal mitochondrial function during tunicamycin-induced ER stress. Tunicamycin has been observed to disrupt the protein levels of HSPA5, ERN1, and EIF2AK3 in TXNDC5-deficient cells. However, palmitic acid has been observed to disrupt the protein levels of ATF6, HSPA5, and EIF2AK3. In conclusion, TXNDC5 can selectively activate distinct ER stress pathways via HSPA5, contingent on the origin of ER stress. Conversely, the absence of TXNDC5 can disrupt the EIF2AK3 cascade.
The Role of Lipid Metabolism Disorders in the Development of Thyroid Cancer
Authors: Lukasiewicz Martyna , Zwara Agata , Kowalski Jacek , Mika Adriana , Hellmann Andrzej
Published: July 2024

Thyroid cancer (TC) is a neoplasm with an increasing incidence worldwide. Its etiology is complex and based on a multi-layered interplay of factors. Among these, disorders of lipid metabolism have emerged as an important area of investigation. Cancer cells are metabolically reprogrammed to promote their rapid growth, proliferation, and survival. This reprogramming is associated with significant changes at the level of lipids, mainly fatty acids (FA), as they play a critical role in maintaining cell structure, facilitating signaling pathways, and providing energy. These lipid-related changes help cancer cells meet the increased demands of continued growth and division while adapting to the tumor microenvironment. In this review, we examine lipid metabolism at different stages, including synthesis, transport, and oxidation, in the context of TC and the effects of obesity and hormones on TC development. Recent scientific efforts have revealed disturbances in lipid homeostasis that are specific to thyroid cancer, opening up potential avenues for early detection and targeted therapeutic interventions. Understanding the intricate metabolic pathways involved in FA metabolism may provide insights into potential interventions to prevent cancer progression and mitigate its effects on surrounding tissues.
Mesenchymal Stem Cell-Secreted Exosomes and Soluble Signals Regulate Breast Cancer Metastatic Dormancy: Current Progress and Future Outlook
Authors: Dai Bei , Clark Amanda M. , Wells Alan
Published: July 2024

Breast cancer is most common in women, and in most cases there is no evidence of spread and the primary tumor is removed, resulting in a ‘cure’. However, in 10% to 30% of these women, distant metastases recur after years to decades. This is due to breast cancer cells disseminating to distant organs and lying quiescent. This is called metastatic dormancy. Dormant cells are generally resistant to chemotherapy, hormone therapy and immunotherapy as they are non-cycling and receive survival signals from their microenvironment. In this state, they are clinically irrelevant. However, risk factors, including aging and inflammation can awaken dormant cells and cause breast cancer recurrences, which may happen even more than ten years after the primary tumor removal. How these breast cancer cells remain in dormancy is being unraveled. A key element appears to be the mesenchymal stem cells in the bone marrow that have been shown to promote breast cancer metastatic dormancy in recent studies. Indirect co-culture, direct co-culture and exosome extraction were conducted to investigate the modes of signal operation. Multiple signaling molecules act in this process including both protein factors and microRNAs. We integrate these studies to summarize current findings and gaps in the field and suggest future research directions for this field.
Broncho-Vaxom Attenuates Lipopolysaccharide-Induced Inflammation in a Mouse Model of Acute Lung Injury
Authors: Woo Min-Seok , Cao Dang Long , Kim Eun-Jin , Jeong Yi Yeong , Kang Dawon
Published: July 2024

Acute lung injury (ALI) is a condition associated with acute respiratory failure, resulting in significant morbidity and mortality. It involves cellular changes such as disruption of the alveolar–capillary membrane, excessive neutrophil migration, and release of inflammatory mediators. Broncho-Vaxom® (BV), a lyophilized product containing cell membrane components derived from eight bacteria commonly found in the respiratory tract, is known for its potential to reduce viral and bacterial lung infections. However, the specific effect of BV on ALI has not been clearly defined. This study explored the preventive effects of BV and its underlying mechanisms in a lipopolysaccharide (LPS)-induced ALI mouse model. Oral BV (1 mg/kg) gavage was administered one hour before the intratracheal injection of LPS to evaluate its preventive effect on the ALI model. The pre-administration of BV significantly mitigates inflammatory parameters, including the production of inflammatory mediators, macrophage infiltration, and NF-κB activation in lung tissue, and the increase in inflammatory cells in bronchoalveolar lavage fluid (BALF). Moreover, BV (3 μg/mL) pretreatment reduced the expression of M1 macrophage markers, interleukins (IL-1β, IL-6), tumor necrosis factor α, and cyclooxygenase-2, which are activated by LPS, in both mouse alveolar macrophage MH-S cells and human macrophage THP-1 cells. These findings showed that BV exhibits anti-inflammatory effects by suppressing inflammatory mediators through the NF-κB pathway, suggesting its potential to attenuate bronchial and pulmonary inflammation.