bluuebunny/crossref_metadata_2025 huggingface dataset:
Lithium: a versatile tool for understanding renal physiology
Authors: Kishore Bellamkonda K. , Ecelbarger Carolyn M.
Published: February 2013

By virtue of its unique interactions with kidney cells, lithium became an important research tool in renal physiology and pathophysiology. Investigators have uncovered the intricate relationships of lithium with the vasopressin and aldosterone systems, and the membrane channels or transporters regulated by them. While doing so, their work has also led to 1) questioning the role of adenylyl cyclase activity and prostaglandins in lithium-induced suppression of aquaporin-2 gene transcription; 2) unraveling the role of purinergic signaling in lithium-induced polyuria; and 3) highlighting the importance of the epithelial sodium channel (ENaC) in lithium-induced nephrogenic diabetes insipidus (NDI). Lithium-induced remodeling of the collecting duct has the potential to shed new light on collecting duct remodeling in disease conditions, such as diabetes insipidus. The finding that lithium inhibits glycogen synthase kinase-3β (GSK3β) has opened an avenue for studies on the role of GSK3β in urinary concentration, and GSK isoforms in renal development. Finally, proteomic and metabolomic profiling of the kidney and urine in rats treated with lithium is providing insights into how the kidney adapts its metabolism in conditions such as acquired NDI and the multifactorial nature of lithium-induced NDI. This review provides state-of-the-art knowledge of lithium as a versatile tool for understanding the molecular physiology of the kidney, and a comprehensive view of how this tool is challenging some of our long-standing concepts in renal physiology, often with paradigm shifts, and presenting paradoxical situations in renal pathophysiology. In addition, this review points to future directions in research where lithium can lead the renal community.
PET/CT imaging of age- and task-associated differences in muscle activity during fatiguing contractions
Authors: Rudroff Thorsten , Kalliokoski Kari K. , Block Derek E. , Gould Jeffrey R. , Klingensmith William C. , Enoka Roger M.
Published: February 2013

The study compared positron emission tomography/computed tomography (PET/CT) of [18F]-2-fluoro-2-deoxy-d-glucose ([18F]-FDG) uptake by skeletal muscles and the amount of muscle activity as indicated by surface electromyographic (EMG) recordings when young and old men performed fatiguing isometric contractions that required either force or position control. EMG signals were recorded from thigh muscles of six young men (26 ± 6 yr) and six old men (77 ± 6 yr) during fatiguing contractions with the knee extensors. PET/CT scans were performed immediately after task failure. Glucose uptake in 24 leg muscles, quantified as standardized uptake values, was greater for the old men after the force task and differed across tasks for the young men (force, 0.64 ± 0.3 g/ml; position, 0.73 ± 0.3 g/ml), but not the old men (force, 0.84 ± 0.3 g/ml; position, 0.79 ± 0.26 g/ml) (age × task interaction; P < 0.001). In contrast, the rate of increase in EMG amplitude for the agonist muscles was greater for the young men during the two contractions and there was no difference for either group of subjects in the rate of increase in EMG amplitude across the two tasks. The imaging estimates of glucose uptake indicated age- and task-dependent differences in the spatial distribution of [18F]-FDG uptake by skeletal muscles during fatiguing contractions. The findings demonstrate that PET/CT imaging of [18F]-FDG uptake, but not surface EMG recordings, detected the modulation of muscle activity across the fatiguing tasks by the young men but not the old men.
A dominant role for mechanical resonance in physiological finger tremor revealed by selective minimization of voluntary drive and movement
Authors: Vernooij Carlijn A. , Reynolds Raymond F. , Lakie Martin
Published: February 2013

There is a debate in the literature about whether the low- and high-frequency peaks of physiological finger tremor are caused by resonance or central drive. One way to address this issue is to examine the consequences of eliminating, as far as possible, the resonant properties or the voluntary drive. To study the effect of minimizing resonance, finger tremor was recorded under isometric conditions and compared with normal isotonic tremor. To minimize central drive, finger tremor was generated artificially by broad-band electrical stimulation. When resonance was minimized, tremor size declined almost monotonically with increasing frequency. There was no consistent large peak at a frequency characteristic of tremor. Although there was sometimes a peak around the tremor frequency during some isometric conditions, it was extremely small and variable; therefore, any contribution of central drive was minimal. In contrast, there was always a prominent peak in the isotonic frequency spectra. Resonance was, therefore, necessary to produce the characteristic tremor peaks. When central drive was minimized by replacing voluntary muscle activation with artificial stimulation, a realistic tremor spectrum was observed. Central drive is, therefore, not required to generate a characteristic physiological tremor spectrum. In addition, regardless of the nature of the driving input (voluntary or artificial), increasing the size of the input considerably reduced isotonic tremor frequency. We attribute the frequency reduction to a movement-related thixotropic change in muscle stiffness. From these results we conclude that physiological finger tremor across a large range of frequencies is produced by natural broad-band forcing of a nonlinear resonant system, and that synchronous central input is not required.
Imaging light responses of retinal ganglion cells in the living mouse eye
Authors: Yin Lu , Geng Ying , Osakada Fumitaka , Sharma Robin , Cetin Ali H. , Callaway Edward M. , Williams David R. , Merigan William H.
Published: February 2013

This study reports development of a novel method for high-resolution in vivo imaging of the function of individual mouse retinal ganglion cells (RGCs) that overcomes many limitations of available methods for recording RGC physiology. The technique combines insertion of a genetically encoded calcium indicator into RGCs with imaging of calcium responses over many days with FACILE (functional adaptive optics cellular imaging in the living eye). FACILE extends the most common method for RGC physiology, in vitro physiology, by allowing repeated imaging of the function of each cell over many sessions and by avoiding damage to the retina during removal from the eye. This makes it possible to track changes in the response of individual cells during morphological development or degeneration. FACILE also overcomes limitations of existing in vivo imaging methods, providing fine spatial and temporal detail, structure-function comparison, and simultaneous analysis of multiple cells.
Release of a single neurotransmitter from an identified interneuron coherently affects motor output on multiple time scales
Authors: Dacks Andrew M. , Weiss Klaudiusz R.
Published: February 2013

Neurotransmitters can have diverse effects that occur over multiple time scales often making the consequences of neurotransmission difficult to predict. To explore the consequences of this diversity, we used the buccal ganglion of Aplysia to examine the effects of GABA release by a single interneuron, B40, on the intrinsic properties and motor output of the radula closure neuron B8. B40 induces a picrotoxin-sensitive fast IPSP lasting milliseconds in B8 and a slow EPSP lasting seconds. We found that the excitatory effects of this slow EPSP are also mediated by GABA. Together, these two GABAergic actions structure B8 firing in a pattern characteristic of ingestive programs. Furthermore, we found that repeated B40 stimulation induces a persistent increase in B8 excitability that was occluded in the presence of the GABA B receptor agonist baclofen, suggesting that GABA affects B8 excitability over multiple time scales. The phasing of B8 activity during the feeding motor programs determines the nature of the behavior elicited during that motor program. The persistent increase in B8 excitability induced by B40 biased the activity of B8 during feeding motor programs causing the motor programs to become more ingestive in nature. Thus, a single transmitter released from a single interneuron can have consequences for motor output that are expressed over multiple time scales. Importantly, despite the differences in their signs and temporal characteristics, the three actions of B40 are coherent in that they promote B8 firing patterns that are characteristic of ingestive motor outputs.
Convection-Enhanced Delivery for Targeted Delivery of Antiglioma Agents: The Translational Experience
Authors: Yun Jonathan , Rothrock Robert J. , Canoll Peter , Bruce Jeffrey N.
Published: February 2013

Recent improvements in the understanding of glioblastoma (GBM) have allowed for increased ability to develop specific, targeted therapies. In parallel, however, there is a need for effective methods of delivery to circumvent the therapeutic obstacles presented by the blood-brain barrier and systemic side effects. The ideal delivery system should allow for adequate targeting of the tumor while minimizing systemic exposure, applicability across a wide range of potential therapies, and have existing safe and efficacious systems that allow for widespread application. Though many alternatives to systemic delivery have been developed, this paper will focus on our experience with convection-enhanced delivery (CED) and our focus on translating this technology from pre-clinical studies to the treatment of human GBM.
FPGA Implementation of Gaussian Mixture Model Algorithm for 47 fps Segmentation of 1080p Video
Authors: Genovese Mariangela , Napoli Ettore , De Caro Davide , Petra Nicola , Strollo Antonio G. M.
Published: February 2013

Circuits and systems able to process high quality video in real time are fundamental in nowadays imaging systems. The circuit proposed in the paper, aimed at the robust identification of the background in video streams, implements the improved formulation of the Gaussian Mixture Model (GMM) algorithm that is included in the OpenCV library. An innovative, hardware oriented, formulation of the GMM equations, the use of truncated binary multipliers, and ROM compression techniques allow reduced hardware complexity and increased processing capability. The proposed circuit has been designed having commercial FPGA devices as target and provides speed and logic resources occupation that overcome previously proposed implementations. The circuit, when implemented on Virtex6 or StratixIV, processes more than 45 frame per second in 1080p format and uses few percent of FPGA logic resources.
The Clinical Obesity Maintenance Model: An Integration of Psychological Constructs including Mood, Emotional Regulation, Disordered Overeating, Habitual Cluster Behaviours, Health Literacy and Cognitive Function
Authors: Raman Jayanthi , Smith Evelyn , Hay Phillipa
Published: February 2013

Psychological distress and deficits in executive functioning are likely to be important barriers to effective weight loss maintenance. The purpose of this paper is twofold. First, in the light of recent evidence in the fields of neuropsychology and obesity, particularly on the deficits in the executive function in overweight and obese individuals, a conceptual and theoretical framework of obesity maintenance is introduced by way of a clinical obesity maintenance model (COMM). It is argued that psychological variables, that of habitual cluster Behaviors, emotional dysregulation, mood, and health literacy, interact with executive functioning and impact on the overeating/binge eating behaviors of obese individuals. Second, cognizant of this model, it is argued that the focus of obesity management should be extended to include a broader range of maintaining mechanisms, including but not limited to cognitive deficits. Finally, a discussion on potential future directions in research and practice using the COMM is provided.
PKM2, a Central Point of Regulation in Cancer Metabolism
Authors: Wong Nicholas , De Melo Jason , Tang Damu
Published: February 2013

Aerobic glycolysis is the dominant metabolic pathway utilized by cancer cells, owing to its ability to divert glucose metabolites from ATP production towards the synthesis of cellular building blocks (nucleotides, amino acids, and lipids) to meet the demands of proliferation. The M2 isoform of pyruvate kinase (PKM2) catalyzes the final and also a rate-limiting reaction in the glycolytic pathway. In the PK family, PKM2 is subjected to a complex regulation by both oncogenes and tumour suppressors, which allows for a fine-tone regulation of PKM2 activity. The less active form of PKM2 drives glucose through the route of aerobic glycolysis, while active PKM2 directs glucose towards oxidative metabolism. Additionally, PKM2 possesses protein tyrosine kinase activity and plays a role in modulating gene expression and thereby contributing to tumorigenesis. We will discuss our current understanding of PKM2's regulation and its many contributions to tumorigenesis.
Catalyst-Free Synthesis of Highly Biologically Active 5-Arylidene Rhodanine and 2,4-Thiazolidinedione Derivatives Using Aldonitrones in Polyethylene Glycol
Authors: Kumar Dhruva , Narwal Suresh , Sandhu Jagir S.
Published: February 2013

A green, efficient synthesis of 5-arylidene rhodanine and 2,4-thiazolidinedione derivatives without using any external catalyst in polyethylene glycol (PEG) at 80°C has been described. Reaction procedure is very simple, short, and obtained yields are very high.
Safety Aspects of People Exposed to Ultra Wideband Radar Fields
Authors: Cavagnaro Marta , Pisa Stefano , Pittella Erika
Published: February 2013

The safety aspects of people exposed to the field emitted by ultra wideband (UWB) radar, operating both in the spatial environment and on ground, for breath activity monitoring are analyzed. The basic restrictions and reference levels reported in the ICNIRP safety guideline are considered, and the compliance of electromagnetic fields radiated by a UWB radar with these limits is evaluated. First, simplified analytical approaches are used; then, both a 3-dimensional multilayered body model and an anatomical model of the head have been used to better evaluate the electromagnetic absorption when a UWB antenna is placed in front of the head. The obtained results show that if the field emitted by the UWB radar is compliant with spatial and/or ground emission masks, then both reference levels and basic restrictions are largely satisfied.
New Tools in HCV Diagnosis, in Light of the Enhanced Awareness and the New Drugs for Treatment: SMARTube and Stimmunology
Authors: Gorodin Svetlana , Unal Serhat , Wang Youchun , Mikhaylov Mikhail I. , Bigbulatova Ludmila , Jehuda-Cohen Tamar
Published: February 2013

With improved HCV therapy, challenges regarding HCV diagnosis, such as seronegative window period, false positive readings, and differentiation between recent, chronic, and resolved infections, are of increasing importance. To
address these challenges an innovative device—SMARTube HIV & HCV—was used. Blood samples were tested for anti‐HCV antibodies before and after incubation in the SMARTube, which promotes the in vitro stimulation of in vivo HCV primed lymphocytes, thus enhancing levels of anti‐HCV antibodies. Comparing antibody levels, in concordant samples before and after SMARTube, yielded the Stimulation Index (SI). Among 5888 fresh blood samples, from various populations and regions worldwide, 641 were seropositive using plasma, while SMARTube processing (yielding enriched plasma, termed SMARTplasma) enabled diagnosis of 10 additional carriers in high‐risk cohorts, that is, earlier detection. Using SMARTplasma eliminated all false positive results, using the current assays. In addition we show that SI calculation may serve as an important tool for differentiating between those who recently seroconverted, carriers of long‐term infection, and those who have cleared the virus. SMARTube and the SI could lead to better, more informative diagnosis of HCV infections and play an important role in changing the way we treat both the infected individuals and the epidemic as a whole.
Thermal and Cure Kinetics of Epoxy Molding Compounds Cured with Thermal Latency Accelerators
Authors: Su Chean-Cheng , Wei Chien-Huan , Li Bo-Ching
Published: February 2013

The cure kinetics and mechanisms of a biphenyl type epoxy molding compounds (EMCs) with thermal latency organophosphine accelerators were studied using differential scanning calorimetry (DSC). Although the use of triphenylphosphine-1,4-benzoquinone (TPP-BQ) and triphenylphosphine (TPP) catalysts in biphenyl type EMCs exhibited autocatalytic mechanisms, thermal latency was higher in the TPP-BQ catalyst in EMCs than in the TPP catalyst in EMCs. Analyses of thermal characteristics indicated that TPP-BQ is inactive at low temperatures. At high temperatures, however, TPP-BQ increases the curing rate of EMC in dynamic and isothermal curing experiments. The reaction of EMCs with the TPP-BQ latent catalyst also had a higher temperature sensitivity compared to the reaction of EMCs with TPP catalyst. In resin transfer molding, EMCs containing the TPP-BQ thermal latency accelerator are least active at a low temperature. Consequently, EMCs have a low melt viscosity before gelation, and the resins and filler are evenly mixed in the kneading process. Additionally, flowability is increased before the EMCs form a network structure in the molding process. The proposed kinetic model adequately describes curing behavior in EMCs cured with two different organophosphine catalysts up to the rubber state in the progress of curing.
Microwave-Assisted Synthesis of Spirofused Heterocycles Using Decatungstodivanadogermanic Heteropoly Acid as a Novel and Reusable Heterogeneous Catalyst under Solvent-Free Conditions
Authors: Jetti Srinivasa Rao , Verma Divya , Jain Shubha
Published: February 2013

Decatungstodivanadogermanic acid (H6GeW10V2O40·22H2O) was synthesized and used as a novel, green heterogeneous catalyst for the synthesis of spirofused heterocycles from one-pot three-component cyclocondensation reaction of a cyclic ketone, aldehyde, and urea in high yields under solvent-free condition in microwave irradiation at 80°C. This catalyst is efficient not only for cyclic ketones, but also for cyclic β-diketones, β-diester, and β-diamide derivatives such as cyclohexanone, dimedone, and Meldrum's acid, or barbituric acid derivatives.
Fractional Variational Iteration Method versus Adomian’s Decomposition Method in Some Fractional Partial Differential Equations
Authors: Song Junqiang , Yin Fukang , Cao Xiaoqun , Lu Fengshun
Published: February 2013

A comparative study is presented about the Adomian’s decomposition method (ADM), variational iteration method (VIM), and fractional variational iteration method (FVIM) in dealing with fractional partial differential equations (FPDEs). The study outlines the significant features of the ADM and FVIM methods. It is found that FVIM is identical to ADM in certain scenarios. Numerical results from three examples demonstrate that FVIM has similar efficiency, convenience, and accuracy like ADM. Moreover, the approximate series are also part of the exact solution while not requiring the evaluation of the Adomian’s polynomials.
Emergy Analysis of Biogas Systems Based on Different Raw Materials
Authors: Wang Yang , Lin Cong , Li Jing , Duan Na , Li Xue , Fu Yanyan
Published: February 2013

Environmental pollution and energy crisis restrict the development of China, and the utilization of renewable technology is an effective strategy to alleviate the damage. Biogas engineering has rapidly developed attributes to solve environmental problems and create a renewable energy product biogas. In this paper, two different biogas plants’ materials were analyzed by emergy method. One of them is a biogas project whose degraded material is feces (BPF system), and the other is the one whose degraded material is corn straw (BPC system). As a result, the ecological‐economic values of BPF and BPC are $28,300/yr and $8,100/yr, respectively. Considering currency, environment, and human inputs, both of the biogas projects have the ability of disposing waste and potential for development. The proportion of biogas output is much more than fertilizer output; so, fertilizer utilization should be emphasized in the future. In comparison, BPF is better than BPC in the aspects of ecological‐economic benefits, environmental benefits, and sustainability. The reason is the difficulty of corn straw seasonal collection and degradation. Thus it is proposed that BPC should be combined with the other raw materials.
Homoclinic Solutions for a Second-Order Nonperiodic Asymptotically Linear Hamiltonian Systems
Authors: Zheng Qiang
Published: February 2013

We establish a new existence result on homoclinic solutions for a second-order nonperiodic Hamiltonian systems. This homoclinic solution is obtained as a limit of solutions of a certain sequence of nil-boundary value problems which are obtained by the minimax methods. Some recent results in the literature are generalized and extended.
Chronic Wounds, Biofilms and Use of Medicinal Larvae
Authors: Cowan Linda J. , Stechmiller Joyce K. , Phillips Priscilla , Yang Qingping , Schultz Gregory
Published: February 2013

Chronic wounds are a significant health problem in the United States, with annual associated costs exceeding $20 billion annually. Traditional wound care consists of surgical debridement, manual irrigation, moisture retentive dressings, and topical and/or systemic antimicrobial therapy. However, despite progress in the science of wound healing, the prevalence and incidence of chronic wounds and their complications are escalating. The presence & complexity of bacterial biofilms in chronic wounds has recently been recognized as a key aspect of non-healing wounds. Bacterial biofilms are sessile colonies of polymicrobial organisms (bacteria, fungus, etc.) enclosed within a self-produced exopolymeric matrix that provides high levels of tolerance to host defenses, antibiotics and antiseptics. Thus, there is a need for alternative therapies to reduce biofilms in chronic wounds. In this report, we present initial findings from in vitro experiments which show that larval debridement therapy with disinfected blow fly larvae (Phaenicia sericata) reduced total CFUs (6-logs) of planktonic and mature biofilms of Pseudomonas aeruginosa or Staphylococcus aureus grown on dermal pig skin explants by 5-logs after 24 hours of exposure, and eliminated biofilms (no measurable CFUs) after 48 hours of exposure.
Structure of Carbonic Layer in Ohmic Contacts: Comparison of Silicon Carbide/Carbon and Carbon/Silicide Interfaces
Authors: Borowicz Paweł , Kuchuk Adrian , Adamus Zbigniew , Borysiewicz Michał , Ekielski Marek , Kamińska Eliana , Piotrowska Anna , Latek Mariusz
Published: February 2013

The structure of carbonic layer in three samples composed of 4H polytype of silicon carbide and the following sequence of layers: carbon/nickel/silicon/nickel/silicon was investigated with Raman spectroscopy. Different thermal treatment of the samples led to differences in the structure of carbonic layer. Raman measurements were performed with visible excitation focused on two interfaces: silicon carbide/carbon and carbon/silicide. The results showed differences in the structure across carbon film although its thickness corresponds to 8/10 graphene layers.
Hierarchical Neural Regression Models for Customer Churn Prediction
Authors: Mohammadi Golshan , Tavakkoli-Moghaddam Reza , Mohammadi Mehrdad
Published: February 2013

As customers are the main assets of each industry, customer churn prediction is becoming a major task for companies to remain in competition with competitors. In the literature, the better applicability and efficiency of hierarchical data mining techniques has been reported. This paper considers three hierarchical models by combining four different data mining techniques for churn prediction, which are backpropagation artificial neural networks (ANN), self-organizing maps (SOM), alpha-cut fuzzyc-means (α-FCM), and Cox proportional hazards regression model. The hierarchical models are ANN + ANN + Cox, SOM + ANN + Cox, andα-FCM + ANN + Cox. In particular, the first component of the models aims to cluster data in two churner and nonchurner groups and also filter out unrepresentative data or outliers. Then, the clustered data as the outputs are used to assign customers to churner and nonchurner groups by the second technique. Finally, the correctly classified data are used to create Cox proportional hazards model. To evaluate the performance of the hierarchical models, an Iranian mobile dataset is considered. The experimental results show that the hierarchical models outperform the single Cox regression baseline model in terms of prediction accuracy, Types I and II errors, RMSE, and MAD metrics. In addition, theα-FCM + ANN + Cox model significantly performs better than the two other hierarchical models.
Amelioration of Collagen-Induced Arthritis in Female Dark Agouti Rats by Glucosamine Treatment
Authors: Haleagrahara Nagaraja , Tudawe Dulanthi , Chakravarthi Srikumar , Kutty Radhakrishnan Ammu
Published: February 2013

The present study assessed the therapeutic efficacy of glucosamine hydrochloride against collagen-induced arthritis in female Dark Agouti rats (DA). Arthritis was induced by intradermaly injecting a collagen and complete Freund’s adjuvant suspension at multiple sites in the rat at a dose of 4 mg/kg of body weight and thereafter followed by two more boosters of the same dose, after the 1st week and 2nd week of primary immunization. After 21 days from the day of primary immunization, the arthritic group rats were given oral supplementation of glucosamine hydrochloride at a dose of 300 mg/kg of body weight until day 45. The arthritic group treated with glucosamine hydrochloride from day 21 to day 45 showed significant reduction in arthritic histopathological changes of the joints, reduction in paw thickness and also a significant decrease in C-reactive protein and TNF-alpha in the serum. Treatment with 300 mg/kg of glucosamine hydrochloride was able to reverse the arthritic changes, hence suggesting that glucosamine has a therapeutic effect against collagen-induced arthritis.
Path Planning for Mobile Objects in Four-Dimension Based on Particle Swarm Optimization Method with Penalty Function
Authors: Ma Yong , Zamirian M. , Yang Yadong , Xu Yanmin , Zhang Jing
Published: February 2013

We present one algorithm based on particle swarm optimization (PSO) with penalty function to determine the conflict-free path for mobile objects in four-dimension (three spatial and one-time dimensions) with obstacles. The shortest path of the mobile object is set as goal function, which is constrained by conflict-free criterion, path smoothness, and velocity and acceleration requirements. This problem is formulated as a calculus of variation problem (CVP). With parametrization method, the CVP is converted to a time-varying nonlinear programming problem (TNLPP). Constraints of TNLPP are transformed to general TNLPP without any constraints through penalty functions. Then, by using a little calculations and applying the algorithm PSO, the solution of the CVP is consequently obtained. Approach efficiency is confirmed by numerical examples.
Metamaterial Sensors
Authors: Yang Jing Jing , Huang Ming , Tang Hao , Zeng Jia , Dong Ling
Published: February 2013

Metamaterials have attracted a great deal of attention due to their intriguing properties, as well as the large potential applications for designing functional devices. In this paper, we review the current status of metamaterial sensors, with an emphasis on the evanescent wave amplification and the accompanying local field enhancement characteristics. Examples of the sensors are given to illustrate the principle and the performance of the metamaterial sensor. The paper concludes with an optimistic outlook regarding the future of metamaterial sensor.
Pervasive Urban Sensing with Large-Scale Mobile Probe Vehicles
Authors: Zhu Yanmin , Liu Xuemei , Wang Yin
Published: February 2013

With the advance of embedded sensing devices, Pervasive Urban Sensing (PUS) with probe vehicles is becoming increasingly practical. A probe vehicle is equipped with onboard sensing devices that detect urban information as the probe vehicle drive across the road network. For example, GPS sensors can detect real-time vehicle status including instant speed and physical position. PUS can provide the general public valuable urban sensing information, such as frequently updated digital maps, and real-time traffic light states. In this paper, we first present the framework of Pervasive Urban Sensing with probe vehicles. Next, we present two cases of urban sensing with probe vehicles. As case one, we discuss the design of a sensing algorithm for detecting the instant state of traffic lights. As case two, we discuss the design of sensing algorithms for recognizing roads by using the vehicular footprints. Some preliminary results of these two cases of urban sensing are presented and discussed.
A Quantitative Structure-Activity Relationship and Molecular Modeling Study on a Series of Biaryl Imidazole Derivatives Acting as H+/K+-ATPase Inhibitors
Authors: Agarwal Neeraj , Bajpai Anubha , Srivastava Vivek , Gupta Satya P.
Published: February 2013

The H+/K+-ATPase or proton pump is a magnesium-dependant enzyme which causes the exchange of a proton against a potassium ion through a membrane. Over activity of this enzyme causes hyperacidity by producing more of hydrochloric acid inside the stomach. This enzyme, therefore, has been found to be a good target for designing compounds to treat hyperacidity. A quantitative structure-activity relationship (QSAR) study has been made on a novel series of biaryl imidazole derivatives acting as H+/K+-ATPase inhibitors. The H+/K+-ATPase inhibition activity of these compounds is found to be significantly correlated with global topological charge indices (GTCIs) and the total polar surface area (TPSA) of the molecules, indicating the involvement of strong electronic interaction between the molecule and the receptor. Based on the correlations obtained, some new H+/K+-ATPase inhibitors are predicted. The docking studies of these predicted compounds exhibit that these compounds will have even better interaction with the receptor than those already marketed. Thus, they can prove more potent drugs for the treatment of hyperacidity.
Bounds of the Neuman-Sándor Mean Using Power and Identric Means
Authors: Chu Yu-Ming , Long Bo-Yong
Published: February 2013

In this paper we find the best possible lower power mean bounds for the Neuman-Sándor mean and present the sharp bounds for the ratio of the Neuman-Sándor and identric means.
Design of a Compact MIMO Antenna Using Coupled Feed for LTE Mobile Applications
Authors: Zhao Xing , Lee Youngki , Choi Jaehoon
Published: February 2013

A compact multi-input multi-output (MIMO) antenna with a coupled feed structure for 4th generation (4G) handsets is proposed for operation in long-term evolution (LTE) band 13 (0.746 GHz–0.787 GHz). The MIMO antenna consists of two symmetrically distributed identical antenna elements. The size of each element is limited to 20 mm × 10 mm × 5 mm (λ0=392 mm at 0.765 GHz), and the separation between different elements is minimized to 15 mm. Each antenna element contains a Z-shaped coupled feed strip and a simple folded monopole-type radiating element. The simple folded radiating element supports two monopole modes (first order) excited at adjacent frequencies to achieve broadband performance. The coupled feed strip effectively modifies impedance matching and maintains good isolation. The proposed antenna has a 6 dB return loss bandwidth of 55 MHz (0.735 GHz–0.79 GHz) and isolation above 12 dB without the use of an additional isolation enhancement element. Moreover, the envelope correlation coefficient (ECC) is maintained below 0.5 over the designed frequency band.
Evaluation of Weight Loss Failure, Medical Outcomes, and Personal Experiences after Roux-en-Y Gastric Bypass: A Critical Analysis
Authors: Hörchner Rogier , Schweitzer Dave
Published: February 2013

Background. Roux-en-Y gastric bypass (RYGB) is considered an effective and well-tolerated surgical procedure. In this retrospective study, we critically assessed efficacy and negative personal experiences (NPEs) after RYGB with a self-administered questionnaire (SAQ). Methods. This questionnaire study included 404 patients who had undergone RYGB. Analysis was performed using data from medical records, referral letters, and SAQs at an average of 33 months after procedure. We evaluated the occurrence of hypertension, CPEP use and type 2 diabetes mellitus (T2DM), the amount of excess weight loss, degree of satisfaction and negative personal experiences (NPEs) related to the procedure, and adherence to a dedicated life style program and (non)attendance to followup. consults after surgery. Results. 42.3% of all SAQs were evaluable for analysis. T2DM remained similar, while hypertension and continuous positive airway pressure (CPAP) use decreased significantly; excess weight loss of ≥40% was reported in 69% and of <40% in 19%, a significant improvement. Absolute weight gain was reported in 10.5%, fatigue in 44.4%, dysphagia in 11.6%, and other NPEs in 7.6%. Dissatisfaction over weight loss was reported in 9.4%. Mean number of follow-up visits was 9.6 per respondent, while nonattendance of any follow-up visit consults occurred in 1.8%. Conclusions. The use of post-RYGB SAQs provided evaluable data in 42.3%. Treatment failure after RYGB appears to be relevant, encouraging the use of SAQ studies in large cohorts.
Bhanupaka: A Green Process in the Preparation of an Indian Ayurvedic Medicine, Lauha Bhasma
Authors: Krishnamachary Balaji , Purushothaman Arun K. , Pemiah Brindha , Krishnaswamy Sridharan , Krishnan Uma Maheswari , Sethuraman Swaminathan , Sekar Rajan K.
Published: February 2013

The use of lauha bhasma, a traditional Indian herbometallic preparation, is in vogue for centuries for the treatment of various ailments related to iron deficiency. The preparation of lauha bhasma requires strict adherence to time‐consuming, well‐practiced, multistage, and multistep procedures. One of the steps is the treatment of purified metallic ingredients with Triphala decoction (the aqueous extract of Indian gooseberry, Chebulic myrobalans, and Beleric myrobalans) in the presence of sunlight (Bhanupaka). The formation of metal complexes due to the reaction of the metallic ingredients with the constituents of Triphala decoction has been ascertained by FTIR spectroscopy and carbon, hydrogen, and nitrogen analyses. Our results demonstrate that Bhanupaka is an essential step in the preparation of lauha bhasma.
A Numerical Algorithm for Solving Stiff Ordinary Differential Equations
Authors: Yatim S. A. M. , Ibrahim Z. B. , Othman K. I. , Suleiman M. B.
Published: February 2013

An advanced method using block backward differentiation formula (BBDF) is introduced with efficient strategy in choosing the step size and order of the method. Variable step and variable order block backward differentiation formula (VSVO-BBDF) approach is applied throughout the numerical computation. The stability regions of the VSVO-BBDF method are investigated and presented in distinct graphs. The improved performances in terms of accuracy and computation time are presented in the numerical results with different sets of test problems. Comparisons are made between the proposed method and MATLAB’s suite of ordinary differential equations (ODEs) solvers, namely, ode15s and ode23s.
Molecular Pathways: JAK/STAT Pathway: Mutations, Inhibitors, and Resistance
Authors: Quintás-Cardama Alfonso , Verstovsek Srdan
Published: February 2013

Aberrant activation of the JAK/STAT pathway has been reported in a variety of disease states, including inflammatory conditions, hematologic malignancies, and solid tumors. For instance, a large proportion of patients with myeloproliferative neoplasms (MPN) carry the acquired gain-of-function JAK2 V617F somatic mutation. This knowledge has dramatically improved our understanding of the pathogenesis of MPNs and has facilitated the development of therapeutics capable of suppressing the constitutive activation of the JAK/STAT pathway, now recognized as a common underlying biologic abnormality in MPNs. Ruxolitinib is an oral JAK1 and JAK2 inhibitor that has recently been approved for the treatment of myelofibrosis and has been tested against other hematologic malignancies. A series of agents with different specificities against different members of the JAK family of proteins is currently undergoing evaluation in clinical trials for patients with MPNs, lymphoma, and solid tumors such as breast or pancreatic cancer. Despite the significant clinical activity exhibited by these agents in myelofibrosis, some patients fail to respond or progress during JAK kinase inhibitor therapy. Recent reports have shed light into the mechanisms of resistance to JAK inhibitor therapy. Several approaches hold promise to overcome such resistance. Clin Cancer Res; 19(8); 1933–40. ©2013 AACR.
Molecular circuits in thrombosis and inflammation
Authors: Esmon Charles
Published: February 2013

Inflammatory cytokines promote the activation of coagulation through the induction of tissue factor, downregulation of thrombomodulin and upregulation of plasminogen activator inhibitor. In addition to these mechanisms, infections can trigger the release of extracellular traps from leukocytes consisting of DNA and histones. Tissue injury results in release of nucleosomes. Either of these histone containing structures activate platelets and form a potent procoagulant surface on polyphosphates secreted from the platelets, thereby augmenting thrombus formation. In addition, the histones can inhibit thrombomodulin function. The combination of augmenting the platelet procoagulant activity and impairing thrombomodulin activity probably explains the microvascular thrombotic problems observed when histones are infused into mice. Of the histones, H4 is the most potent in all of these activities. DNAase or blocking histone H4 can decrease the thrombotic response initiated by either the extracellular traps or nucleosomes. In addition to the direct prothrombotic activity of histone-DNA complexes, the complexes trigger activation of the toll-like receptors 2, 4 and 9 thereby increasing inflammatory cytokine formation and fostering thrombotic responses through the mechanisms mentioned previously. Furthermore, these cytokines are likely to increase cell necrosis and apoptosis releasing nucleosomes and further augmenting the activation of leukocytes with the subsequent release of extracellular traps. Blocking this histone-mediated cascade has the potential to impact a variety of clinical conditions including sepsis, trauma, chemical toxicity, transplant injury and reperfusion injury.
Non-invasive imaging for studying anti-angiogenic therapy effects
Authors: Ehling Josef , Lammers Twan , Kiessling Fabian
Published: February 2013

Noninvasive imaging plays an emerging role in preclinical and clinical cancer research and has high potential to improve clinical translation of new drugs. This article summarises and discusses tools and methods to image tumour angiogenesis and monitor anti-angiogenic therapy effects. In this context, micro-computed tomography (?CT) is recommended to visualise and quantify the micro-architecture of functional tumour vessels. Contrast-enhanced ultrasound (US) and magnetic resonance imaging (MRI) are favourable tools to assess functional vascular parameters, such as perfusion and relative blood volume. These functional parameters have been shown to indicate antiangiogenic therapy response at an early stage, before changes in tumour size appear. For tumour characterisation, the imaging of the molecular characteristics of tumour blood vessels, such as receptor expression, might have an even higher diagnostic potential and has been shown to be highly suitable for therapy monitoring as well. In this context, US using targeted microbubbles is currently evaluated in clinical trials as an important tool for the molecular characterisation of the angiogenic endothelium. Other modalities, being preferably used for molecular imaging of vessels and their surrounding stroma, are photoacoustic imaging (PAI), near-infrared fluorescence optical imaging (OI), MRI, positron emission tomography (PET) and single photon emission computed tomography (SPECT). The latter two are particularly useful if very high sensitivity is needed, and/or if the molecular target is difficult to access. Carefully considering the pros and cons of different imaging modalities in a multimodal imaging setup enables a comprehensive longitudinal assessment of the (micro)morphology, function and molecular regulation of tumour vessels.
The effect of different hormonal contraceptives on plasma levels of free protein S and free TFPI
Authors: Helmerhorst Frans M. , Fleischer Kathrin , Dahm Anders E. A. , Rosendaal Frits R. , Rosing Jan , Reitsma Pieter , Sandset Per , van Vliet Huib A. A. M. , Raps Marjolein
Published: February 2013

Use of combined oral contraceptives is associated with a three- to sixfold increased risk of venous thrombosis. Hormonal contraceptives induce acquired resistance to activated protein C (APC), which predicts the risk of venous thrombosis. The biological basis of the acquired APC resistance is unknown. Free protein S (PS) and free tissue factor pathway inhibitor (TFPI) are the two main determinants of APC. Our objective was to assess the effect of both hormonal and non-hormonal contraceptives with different routes of administration on free TFPI and free PS levels. We conducted an observational study in 243 users of different contraceptives and measured APC sensitivity ratios (nAPCsr), free TFPI and free PS levels. Users of contraceptives with the highest risk of venous thrombosis as reported in recent literature, had the lowest free TFPI and free PS levels, and vice versa, women who used contraceptives with the lowest risk of venous thrombosis had the highest free TFPI and free PS levels. An association was observed between levels of free TFPI and nAPCsr, and between free PS and nAPCsr. The effect of oral contraceptives on TFPI and PS is a possible explanation for the increased risk of venous thrombosis associated with oral contraceptives.
Alloantibodies against the B subunit of plasma factor XIII developed in its congenital deficiency
Authors: Matsumoto Rui , Sugihara Takashi , Wada Hideho , Souri Masayoshi , Ichinose Akitada
Published: February 2013

Factor XIII (FXIII) is a fibrin-stabilising factor consisting of catalytic A subunits (FXIII-A) and carrier B subunits (FXIII-B). FXIII-B prevents the fast clearance of FXIII-A from the circulation. Congenital FXIII-A deficiency is a rare bleeding disorder, and congenital FXIII-B deficiency is even rarer. Through our recent nationwide survey on “acquired haemophilia-like disease due to anti-FXIII autoantibodies,” we newly diagnosed severe congenital FXIII-B deficiency in a Japanese man. He developed thrombocytopenia and gingival bleedings at the age of 73, and his FXIII activity was as low as 10% of the normal. When he suddenly developed spontaneous intramuscular haematoma, the bleeding was arrested by infusing FXIII concentrates. However, his FXIII activity remained around 10% of the normal. At the age of 74, ELISA and western blotting assay unexpectedly revealed complete absence of FXIII-B in the patient’s plasma. A dot blot assay detected anti-FXIII-B alloantibodies for the first time in this disease, which could be attributed to the infusion of exogenous FXIII. He was found to be homozygous for a Japanese founder-effect mutation of F13B. Repeated infusions of exogenous FXIII for hemostasis increased anti-FXIII-B alloantibodies that resisted FXIII substitution. To the best knowledge of the authors, none of the remaining 10 reported cases of congenital FXIII-B deficiency developed alloantibodies to exogenous FXIII-B of plasma FXIII. An originally mild bleeding phenotype of severe congenital FXIII-B deficiency can be exaggerated by additional acquired conditions. Physicians should consider congenital FXIII-B deficiency when they encounter cases of unexplained bleeding disorders.
Role of Leptin Signaling in the Pathogenesis of Angiotensin II–Mediated Atrial Fibrosis and Fibrillation
Authors: Fukui Akira , Takahashi Naohiko , Nakada Chisato , Masaki Takayuki , Kume Osamu , Shinohara Tetsuji , Teshima Yasushi , Hara Masahide , Saikawa Tetsunori
Published: February 2013

Background—

We examined the hypothesis that leptin signaling contributes to the atrial fibrosis and atrial fibrillation (AF) evoked by angiotensin II (AngII).

Methods and Results—

Eight-week-old male CL57/B6 (CNT) and leptin-deficient ob/ob mice (Ob) were subcutaneously infused with AngII (2.0 mg/kg per day). Two weeks later, transesophageal burst pacing and an electrophysiological study using isolated perfused hearts were performed. Left-atrial tissues were collected to determine interstitial fibrosis by Masson trichrome staining, and the expressions of mRNAs related to inflammatory profibrotic signals were assessed. Left-atrial fibroblasts were isolated from adult Sprague-Dawley and Zucker rats. The effects of leptin (100 ng/mL) or AngII (100 nmol/L) treatment were evaluated. In CNT-AngII mice, leptin expression in the left atrium was upregulated (
P
<0.01). Transesophageal burst pacing induced atrial fibrillation in 88% (7/8) of CNT-AngII mice, but not in Ob-AngII mice (0/8;
P
<0.01). In isolated perfused hearts, atrial fibrillation was induced only in CNT-AngII mice (4/6; 67%). Interatrial conduction time was prolonged in CNT-AngII mice (
P
<0.01), but not in Ob-AngII mice. The upregulation of collagen 1, collagen 3, transforming growth factor-β1, α-smooth muscle actin, MCP-1, F4/80, and RANTES mRNA, which was seen in CNT-AngII mice, was attenuated in Ob-AngII mice. In cultured Sprague-Dawley rat atrial fibroblasts, AngII treatment increased leptin expression (
P
<0.01). Addition of leptin increased transforming growth factor-β1, α-smooth muscle actin, MCP-1, and RANTES expressions in Sprague-Dawley rat atrial fibroblasts, but not in Zucker rat atrial fibroblasts.

Conclusions—

Our results demonstrate for the first time that leptin signaling is essential for the development of atrial fibrosis and atrial fibrillation evoked by AngII.