bluuebunny/crossref_metadata_2025 huggingface dataset:
An RSSI Classification and Tracing Algorithm to Improve Trilateration-Based Positioning
Authors: Shi Yong , Shi Wenzhong , Liu Xintao , Xiao Xianjian
Published: July 2020

Received signal strength indicator (RSSI)-based positioning is suitable for large-scale applications due to its advantages of low cost and high accuracy. However, it suffers from low stability because RSSI is easily blocked and easily interfered with by objects and environmental effects. Therefore, this paper proposed a tri-partition RSSI classification and its tracing algorithm as an RSSI filter. The proposed filter shows an available feature, where small test RSSI samples gain a low deviation of less than 1 dBm from a large RSSI sample collected about 10 min, and the sub-classification RSSIs conform to normal distribution when the minimum sample count is greater than 20. The proposed filter also offers several advantages compared to the mean filter, including lower variance range with an overall range of around 1 dBm, 25.9% decreased sample variance, and 65% probability of mitigating RSSI left-skewness. We experimentally confirmed the proposed filter worked in the path-loss exponent fitting and location computing, and a 4.45-fold improvement in positioning stability based on the sample standard variance, and positioning accuracy improved by 20.5% with an overall error of less than 1.46 m.
Impacts of Moringa oleifera Foliage Substituted for Concentrate Feed on Growth, Nutrient Digestibility, Hematological Attributes, and Blood Minerals of Growing Goats under Abu Dhabi Conditions
Authors: Zaher Hany , Alawaash Saeed , Tolba Amir , Swelum Ayman , Abd El-Hack Mohamed , Taha Ayman , Abdelnour Sameh
Published: July 2020

To avoid the depletion and degradation of natural resources and to maintain long-term environmental quality and sustainability, the present study investigated the impacts of Moringa oleifera foliage (MF) as a replacement for concentrate feed on growth performance, blood hematology, serum metabolites, and serum mineral profile in growing goats. A total of 30 growing local goats (4 months old ± 7 days, with an average weight of 15.80 kg ± 147 g), were randomly allotted to five treatment groups comprising 0%, 25%, 50%, 75%, or 100% of MF replacing the concentrate feed as daily feed. The MF75 and MF100 percent diets decreased (p < 0.05) final body weight (FBW), average daily gain (ADG), and crude protein (CP) of the diet. Moreover, MF up to 75% improved acid detergent fiber (ADF) digestibility. Feeding MF at different levels did not have any deleterious effects on blood chemistry parameters. Urea, low-density lipoprotein (LDL), and alkaline phosphatase (ALP) values were depressed (p < 0.05), due to increasing the MF (MF50, MF75, and MF100) level in the diet of growing goats kept in an arid region, when compared to the control group. Both of MF75 and MF100 led to a decrease (p < 0.05) in phosphorous (P) level, compared with the other groups. The results were interpreted that feeding MF to replace 25% DM of the concentrate feed had no adverse effects on growth performance, feed utilization, serum metabolites, and serum minerals in growing goats reared under arid conditions. The increasing of MF level up to 50% or more significantly altered ADG and blood levels of creatinine, urea and ALP.
Monitoring the Variations of Soil Salinity in a Palm Grove in Southern Algeria
Authors: Benslama Abderraouf , Khanchoul Kamel , Benbrahim Fouzi , Boubehziz Sana , Chikhi Faredj , Navarro-Pedreño Jose
Published: July 2020

Soil salinity is considered the most serious socio-economic and environmental problem in arid and semi-arid regions. This study was done to estimate the soil salinity and monitor the changes in an irrigated palm grove (42 ha) that produces dates of a high quality. Topsoil samples (45 points), were taken during two different periods (May and November), the electrical conductivity (EC) and Sodium Adsorption Ratio (SAR) were determined to assess the salinity of the soil. The results of the soil analysis were interpolated using two geostatistical methods: inverse distance weighting (IDW) and ordinary Kriging (OK). The efficiency and best model of these two methods was evaluated by calculating the mean error (ME) and root mean square error (RMSE), showing that the ME of both interpolation methods was satisfactory for EC (−0.003, 0.145) and for SAR (−0.03, −0.18), but the RMSE value was lower using the IDW with both data and periods. This can explain the accuracy of the IDW interpolation method. This model showed a dominance of soil salinity distribution in the South and South-East of the study area during the first season, and for the second season, the salts were concentrated in the middle of the area. Several factors could interact in this variation such as the topographic direction of the water flow and the aridity of the climate (evaporation). From this study emerges the need to maintain a better management of agricultural water and soils, avoiding salt accumulation, to ensure a good yield and the sustainability of agriculture in arid environments.
Forest Cover Change and the Effectiveness of Protected Areas in the Himalaya since 1998
Authors: Gu Changjun , Zhao Pei , Chen Qiong , Li Shicheng , Li Lanhui , Liu Linshan , Zhang Yili
Published: July 2020

Himalaya, a global biodiversity hotspot, has undergone considerable forest cover fluctuation in recent decades, and numerous protected areas (PAs) have been established to prohibit forest degradation there. However, the spatiotemporal characteristics of this forest cover change across the whole region are still unknown, as are the effectiveness of its PAs. Therefore, here, we first mapped the forest cover of Himalaya in 1998, 2008, and 2018 with high accuracy (>90%) using a random forest (RF) algorithm based on Google Earth Engine (GEE) platform. The propensity score matching (PSM) method was applied with eight control variables to balance the heterogeneity of land characteristics inside and outside PAs. The effectiveness of PAs in Himalaya was quantified based on matched samples. The results showed that the forest cover in Himalaya increased by 4983.65 km2 from 1998 to 2008, but decreased by 4732.71 km2 from 2008 to 2018. Further analysis revealed that deforestation and reforestation mainly occurred at the edge of forest tracts, with over 55% of forest fluctuation occurring below a 2000 m elevation. Forest cover changes in PAs of Himalaya were analyzed; these results indicated that about 56% of PAs had a decreasing trend from 1998 to 2018, including the Torsa (Ia PA), an area representative of the most natural conditions, which is strictly protected. Even so, as a whole, PAs in Himalaya played a positive role in halting deforestation.
Integrating Sustainability into Corporate Strategy: A Case Study of the Textile and Clothing Industry
Authors: Peters Juliane , Simaens Ana
Published: July 2020

This paper aims to explore a set of institutional, organizational, and individual drivers of and barriers to the integration of sustainability into the corporate strategy of a European textile and clothing (T&C) company. The methodology is based on a case study of the exemplar VAUDE, a family-owned sustainable outdoor outfitter company. The results are in accordance with institutional theory and stakeholder theory as a theoretical framework explaining why companies deal with sustainability. The determined drivers depend on coherence at all levels of analysis, i.e., institutional, organizational, and individual. The barriers found are of an institutional and organizational nature only. The findings present significant practical implications for other T&C companies that seek to integrate sustainability into their corporate strategy and for the T&C industry to create a sustainability-friendly environment to drive more companies to become sustainable. It further supports T&C companies in identifying potential barriers, determining how to overcome them, and successfully integrating sustainability into their corporate strategy. To conclude, the results suggest that it only works if sustainability is strongly integrated into the corporate strategy and deeply anchored in all departments and daily tasks of a T&C company.
Intention to Use Accounting Platforms in Romania: A Quantitative Study on Sustainability and Social Influence
Authors: Cokins Gary , Oncioiu Ionica , Türkeș Mirela Cătălina , Topor Dan Ioan , Căpuşneanu Sorinel , Paștiu Carmen Adina , Deliu Delia , Solovăstru Alina Nicoleta
Published: July 2020

Technological innovation evolves through the use of information systems and this study highlights the intentions of using accounting platforms by conducting a quantitative study on sustainability and its social influence. The purpose of this study is to investigate the factors that influence the acceptance and use by company managers of accounting platforms available online by developing the unified theory of acceptance and use of technology (UTAUT) construct model. The research method used in the quantitative study was the sample survey, using the online questionnaire as a data collection tool. A number of 401 companies provided complete and useful answers to this research. The major contributions of the research were the use of two variables considered significant in the study: perceived credibility (PC) and perception of risk (PR). The results of the study showed that performance expectancy (PE), social influence (SI), and perceived risk (PR) have a positive influence on the intention to use the accounting platforms available online, while perceived credibility shows a much more significant and stronger influence.
Evaluation of Evacuation Strategies According to the Travel Demand: The Case of Nuclear Research Reactor HANARO’s EPZ
Authors: Kim Bongseok , Jeon Hyeonmyeong , Son Bongsoo
Published: July 2020

In the event of a nuclear accident, evacuation is the most effective protective action for the public. During the evacuation, total travel time is a key measure to protect the public because it is directly related to the public’s radiation exposure. Thus, strategies that reduce the total travel time are needed for a safer nuclear emergency plan. Many studies on evacuation strategies so far have suggested the methodology of effective routing decisions or delay management. Despite the application of those strategies during evacuation, the effectiveness of those strategies, in reality, varies depending on the level of travel demand. In this study, evacuation strategies based on travel demand levels were evaluated based on the case of the Emergency Planning Zone (EPZ) of HANARO, the nuclear research reactor in the Republic of Korea. As a result, it was confirmed that effective evacuation strategies could be applied differently according to travel demand levels.
A Simulator to Determine the Evolution of Disparities in Food Consumption between Socio-Economic Groups: A Brazilian Case Study
Authors: Gerber Machado Pedro , Tomei Julia , Hawkes Adam , de Oliveira Ribeiro Celma
Published: July 2020

Food is a fundamental right that deserves attention but is usually dealt with from the supply side in aggregated models that use macroeconomic variables to forecast the demand and the required supply. This study challenges this paradigm by developing a simulator to analyze food consumption from the demand side and estimate the evolution of disparity in food consumption over time with respect to region, sex, ethnicity, education, and income. This novel approach was applied to Brazil using household expenditure surveys to feed serial neural networks. Results show that the ‘poorer’ north and northeast of Brazil encounter the lowest consumption of food and are therefore the most food vulnerable regions. This trend continues to 2040. The ‘richer’ south and southeast regions have higher food consumption, which varies according to sex, ethnicity, education, and income. Brazil has contrasting issues with some groups having considerably higher food consumption, while other groups still have less than the threshold for healthy consumption. Now, the country not only has to deal with the food access by the most vulnerable due to the latest economic declines but also to deal with excess consumption, the so-called “double burden of malnutrition”.
A Mini-Review of Urban Wastewater Treatment in Greece: History, Development and Future Challenges
Authors: Prochaska Charikleia , Zouboulis Anastasios
Published: July 2020

Although Greece has accomplished wastewater infrastructure construction to a large extent, as 91% of the country’s population is already connected to urban wastewater treatment plants (WWTPs), many problems still need to be faced. These include the limited reuse of treated wastewater and of the surplus sludge (biosolids) produced, the relative higher energy consumption in the existing rather aged WWTPs infrastructure, and the proper management of failing or inadequately designed septic tank/soil absorption systems, still in use in several (mostly rural) areas, lacking sewerage systems. Moreover, the wastewater treatment sector should be examined in the general framework of sustainable environmental development; therefore, Greece’s future challenges in this sector ought to be reconsidered. Thus, the review of Greece’s urban wastewater history, even from the ancient times, up to current developments and trends, will be shortly addressed. This study also notes that the remaining challenges should be analyzed in respect to the country’s specific needs (e.g., interaction with the extensive tourism sector), as well as to the European Union’s relevant framework policies and to the respective international technological trends, aiming to consider the WWTPs not only as sites for the treatment/removal of pollutants to prevent environmental pollution, but also as industrial places where energy is efficiently used (or even produced), resources’ content can be potentially recovered and reused (e.g., nutrients, treated water, biosolids), and environmental sustainability is being practiced overall.
Lorentz Invariance Violation Tests in Astroparticle Physics
Authors: Martínez-Huerta Humberto , Lang Rodrigo Guedes , de Souza Vitor
Published: July 2020

In this review, we present the latest exclusion limits obtained from astroparticles on Lorentz Invariance Violation (LIV) in the photon sector. We discuss the techniques known as energy-dependent time delay or time lag, subluminal pair production threshold shift, suppression of air shower formation, superluminal photon decay, and superluminal photon splitting. Perspectives for future results on LIV with the next generation of experiments are also addressed.
Bimetallic Mo–Fe Co-Catalyst-Based Nano-Carbon Impregnated on PAC for Optimum Super-Hydrophobicity
Authors: Betar Bashir O , Alsaadi Mohammed A , Chowdhury Zaira Z. , Aroua Mohamed K , Mjalli Farouq S. , Dimyati Kaharudin , Hindia MHD N , Elfghi Fawzi M. , Ahmed Yehya M. , Abbas Hazim F
Published: July 2020

The application of super-hydrophobic nanomaterials for synthesizing membranes with unique physiochemical properties has gained a lot of interest among researchers. The presence of super-hydrophobic materials inside the membrane matrix can play a vital role not only in the separation of toxins, but also to achieve higher water flux with lower fouling tendencies required for an efficient membrane distillation process. In this research, super-hydrophobic carbon nanomaterials (CNMs) were synthesized using powder activated carbon (PAC) as a precursor, whereby the growth was initiated using a bimetallic catalyst of iron (Fe) and molybdenum (Mo). Until recently, no research has been conducted for synthesis and to observe the catalytic influence of bimetallic catalysts on the physiochemical characteristics of the derived CNMs. The synthesis process was carried out using the chemical vapor deposition (CVD) process. The CVD process was optimized using Box–Behnken factorial design (BBD), whereby 15 experiments were carried out under different conditions. Three input variables, which were percentage composition of catalysts (percentage of Fe and Mo) and reaction time (tr), were optimized with respect to their impact on the desired percentage output of yield (CY) and contact angle (CA). Analysis of variance (ANOVA) testing was carried out. It was observed that the developed model was statistically significant. The highest CY (320%) and CA (172°) were obtained at the optimal loading of 5% Fe and 2% Mo, with a reaction time of 40 min. Surface morphological features were observed using field emission scanning electron microscopic (FESEM) and transmission electron microscopic (TEM) analysis. The images obtained from FESEM and TEM revealed the presence of two types of CNMs, including carbon nanofibers (CNFs) and multiwall carbon nanotubes (CNTs). Thermogravimetric analysis was carried out to observe the temperature degradation profile of the synthesized sample. Raman spectroscopic analysis was also used in order to have a better understanding regarding the proportion of ordered and disordered carbon content inside the synthesized sample.
Off-Shell Quantum Fields to Connect Dressed Photons with Cosmology
Authors: Sakuma Hirofumi , Ojima Izumi , Ohtsu Motoichi , Ochiai Hiroyuki
Published: July 2020

The anomalous nanoscale electromagnetic field arising from light–matter interactions in a nanometric space is called a dressed photon. While the generic technology realized by utilizing dressed photons has demolished the conventional wisdom of optics, for example, the unexpectedly high-power light emission from indirect-transition type semiconductors, dressed photons are still considered to be too elusive to justify because conventional optical theory has never explained the mechanism causing them. The situation seems to be quite similar to that of the dark energy/matter issue in cosmology. Regarding these riddles in different disciplines, we find a common important clue for their resolution in the form of the relevance of space-like momentum support, without which quantum fields cannot interact with each other according to a mathematical result of axiomatic quantum field theory. Here, we show that a dressed photon, as well as dark energy, can be explained in terms of newly identified space-like momenta of the electromagnetic field and dark matter can be explained as the off-shell energy of the Weyl tensor field.
Explicit Properties of q-Cosine and q-Sine Euler Polynomials Containing Symmetric Structures
Authors: Ryoo Cheon Seoung , Kang Jung Yoog
Published: July 2020

In this paper, we introduce q-cosine and q-sine Euler polynomials and determine identities for these polynomials. From these polynomials, we obtain some special properties using a power series of q-trigonometric functions, properties of q-exponential functions, and q-analogues of the binomial theorem. We investigate the approximate roots of q-cosine Euler polynomials that help us understand these polynomials. Moreover, we display the approximate roots movements of q-cosine Euler polynomials in a complex plane using the Newton method.
Skew Generalized Normal Innovations for the AR(p) Process Endorsing Asymmetry
Authors: Neethling Ané , Ferreira Johan , Bekker Andriëtte , Naderi Mehrdad
Published: July 2020

The assumption of symmetry is often incorrect in real-life statistical modeling due to asymmetric behavior in the data. This implies a departure from the well-known assumption of normality defined for innovations in time series processes. In this paper, the autoregressive (AR) process of order p (i.e., the AR(p) process) is of particular interest using the skew generalized normal (SGN) distribution for the innovations, referred to hereafter as the ARSGN(p) process, to accommodate asymmetric behavior. This behavior presents itself by investigating some properties of the SGN distribution, which is a fundamental element for AR modeling of real data that exhibits non-normal behavior. Simulation studies illustrate the asymmetry and statistical properties of the conditional maximum likelihood (ML) parameters for the ARSGN(p) model. It is concluded that the ARSGN(p) model accounts well for time series processes exhibiting asymmetry, kurtosis, and heavy tails. Real time series datasets are analyzed, and the results of the ARSGN(p) model are compared to previously proposed models. The findings here state the effectiveness and viability of relaxing the normal assumption and the value added for considering the candidacy of the SGN for AR time series processes.
3D-MRI Brain Tumor Detection Model Using Modified Version of Level Set Segmentation Based on Dragonfly Algorithm
Authors: Khalil Hassan A. , Darwish Saad , Ibrahim Yasmine M. , Hassan Osama F.
Published: July 2020

Accurate brain tumor segmentation from 3D Magnetic Resonance Imaging (3D-MRI) is an important method for obtaining information required for diagnosis and disease therapy planning. Variation in the brain tumor’s size, structure, and form is one of the main challenges in tumor segmentation, and selecting the initial contour plays a significant role in reducing the segmentation error and the number of iterations in the level set method. To overcome this issue, this paper suggests a two-step dragonfly algorithm (DA) clustering technique to extract initial contour points accurately. The brain is extracted from the head in the preprocessing step, then tumor edges are extracted using the two-step DA, and these extracted edges are used as an initial contour for the MRI sequence. Lastly, the tumor region is extracted from all volume slices using a level set segmentation method. The results of applying the proposed technique on 3D-MRI images from the multimodal brain tumor segmentation challenge (BRATS) 2017 dataset show that the proposed method for brain tumor segmentation is comparable to the state-of-the-art methods.
Production of Paralytic Shellfish Toxins (PSTs) in Toxic Alexandrium catenella is Intertwined with Photosynthesis and Energy Production
Authors: Tse Sirius Pui-kam , Lee Fred Wang-fat , Mak Daniel Yun-lam , Kong Hang-kin , Chan Kenrick Kai-yuen , Lo Pak-yeung , Lo Samuel Chun-lap
Published: July 2020

To investigate the mechanism for the production of paralytic shellfish toxins (PST) in toxic dinoflagellates, with a 2D-gel based approach, we had made two sets of proteomic comparisons: (a) between a toxic Alexandrium catenella (AC-T) and a phylogenetically closely related non-toxic strain (AC-N), (b) between toxic AC-T grown in a medium with 10% normal amount of phosphate (AC-T-10%P) known to induce higher toxicity and AC-T grown in normal medium. We found that photosynthesis and energy production related proteins were up-regulated in AC-T when compared to AC-N. However, the same group of proteins was down-regulated in AC-T-10%P when compared to normal AC-T. Examining the relationship of photosynthesis and toxin content of AC-T upon continuous photoperiod experiment revealed that while growth and associated toxin content increased after 8 days of continuous light, toxin content maintained constant when cells were shifted from continuous light to continuous dark for 3 days. This emphasized the cruciality of light availability on toxin biosynthesis in AC-T, while another light-independent mechanism may be responsible for higher toxicity in AC-T-10%P compared to normal AC-T. Taken all together, it is believed that the interplay between “illumination”, “photosynthesis”, “phosphate availability”, and “toxin production” is much more complicated than what we had previously anticipated.
Mycotoxin Contamination Management Tools and Efficient Strategies in Feed Industry
Authors: Cheli Federica
Published: July 2020

n/a
Enhanced Antimicrobial Activity of N-Terminal Derivatives of a Novel Brevinin-1 Peptide from The Skin Secretion of Odorrana schmackeri
Authors: Zhou Xiaowei , Liu Yue , Gao Yitian , Wang Yuanxing , Xia Qiang , Zhong Ruimin , Ma Chengbang , Zhou Mei , Xi Xinping , Shaw Chris , Chen Tianbao , Wu Di , Kwok Hang Fai , Wang Lei
Published: July 2020

Antimicrobial peptides (AMPs) are promising therapeutic alternatives compared to conventional antibiotics for the treatment of drug-resistant bacterial infections. However, the application of the overwhelming majority of AMPs is limited because of the high toxicity and high manufacturing costs. Amphibian skin secretion has been proven to be a promising source for the discovery and development of novel AMPs. Herein, we discovered a novel AMP from the skin secretion of Odorrana schmackeri, and designed the analogues by altering the key factors, including conformation, net charge and amphipathicity, to generate short AMPs with enhanced therapeutic efficacy. All the peptides were chemically synthesised, followed by evaluating their biological activity, stability and cytotoxicity. OSd, OSe and OSf exhibited broad-spectrum antibacterial effects, especially OSf, which presented the highest therapeutic index for the tested bacteria. Moreover, these peptides displayed good stability. The results from scanning electron microscopy and transmission electron microscopy studies, indicated that brevinin-OS, OSd, OSe and OSf possessed rapid bactericidal ability by disturbing membrane permeability and causing the release of cytoplasmic contents. In addition, OSd, OSe and OSf dramatically decreased the mortality of waxworms acutely infected with MRSA. Taken together, these data suggested that a balance between positive charge, degrees of α-helicity and hydrophobicity, is necessary for maintaining antimicrobial activity, and these data successfully contributed to the design of short AMPs with significant bactericidal activity and cell selectivity.
Profiling Transcripts of Vector Competence between Two Different Aedes aegypti Populations in Florida
Authors: Shin Dongyoung , Kang Seokyoung , Smartt Chelsea T.
Published: July 2020

A Chikungunya virus (CHIKV) outbreak in Italy in 2007 spread to include the islands of the Caribbean and most of the Americas and still circulates in Europe and Africa. Florida being close in distance to the Caribbean islands experienced a CHIKV outbreak in 2014 and continues to have a few travel-related cases each year. It is known that different environmental conditions in different regions can result in genetic variation that favor changes in competence to arbovirus. We evaluated the vector competence of Florida Aedes aegypti for CHIKV and determined if there is a geographic component that influences genes involved in CHIKV competence. We utilized a genomic approach to identify the candidate genes using RNA sequencing. The infection and dissemination results showed that field populations were more competent vectors for CHIKV than a lab population. The differentially expressed genes in the two field-collected CHIKV-infected populations, compared to the Rockefeller strain, were related to the Wnt/Notch signaling pathway, with similarity to genes scattered throughout the signaling pathway. This result suggested the possibility of identifying genes involved in the determination of vector competence in different gene pools of Ae. aegypti.
Persistent BK Polyomavirus Viruria Is Associated with Accumulation of VP1 Mutations and Neutralization Escape
Authors: McIlroy Dorian , Hönemann Mario , Nguyen Ngoc-Khanh , Barbier Paul , Peltier Cécile , Rodallec Audrey , Halary Franck , Przyrowski Emilie , Liebert Uwe , Hourmant Maryvonne , Bressollette-Bodin Céline
Published: July 2020

To investigate the relationship between neutralization escape and persistent high-level BK polyomavirus replication after kidney transplant (KTx), VP1 sequences were determined by Sanger and next-generation sequencing in longitudinal samples from KTx recipients with persistent high-level viruria (non-controllers) compared to patients who suppressed viruria (controllers). The infectivity and neutralization resistance of representative VP1 mutants were investigated using pseudotype viruses. In all patients, the virus population was initially dominated by wild-type VP1 sequences, then non-synonymous VP1 mutations accumulated over time in non-controllers. BC-loop mutations resulted in reduced infectivity in 293TT cells and conferred neutralization escape from cognate serum in five out of six non-controller patients studied. When taken as a group, non-controller sera were not more susceptible to neutralization escape than controller sera, so serological profiling cannot predict subsequent control of virus replication. However, at an individual level, in three non-controller patients the VP1 variants that emerged exploited specific “holes” in the patient’s humoral response. Persistent high-level BK polyomavirus replication in KTx recipients is therefore associated with the accumulation of VP1 mutations that can confer resistance to neutralization, implying that future BKPyV therapies involving IVIG or monoclonal antibodies may be more effective when used as preventive or pre-emptive, rather than curative, strategies.
Crocetin Improves Dengue Virus-Induced Liver Injury
Authors: Sreekanth Gopinathan Pillai , Chuncharunee Aporn , Yenchitsomanus Pa-thai , Limjindaporn Thawornchai
Published: July 2020

Dengue virus (DENV) infection is one of the most widespread mosquito-borne viral infections. Liver injury is commonly observed in severe DENV infection, and the present study aimed to examine the efficacy of crocetin treatment in an immunocompetent mouse model of DENV infection exhibiting liver injury. The efficacy of crocetin treatment in DENV-induced liver injury was assessed via both transaminase levels and histopathology analysis. A real-time polymerase chain reaction array was then used to describe the expression of 84 apoptosis-related genes. Using real-time RT-PCR and Western blot analysis, the gene expressions of host factors were investigated. Additionally, the effect of crocetin in NF-kB signaling during DENV infection was studied. We did not observe any significant reduction in virus production when DENV-infected mice were treated with crocetin. However, DENV-infected mice treated with crocetin showed reduced DENV-induced apoptosis. The real-time polymerase chain reaction array revealed pro-inflammatory cytokine expressions to be significantly reduced in the crocetin-treated DENV-infected mice. We also found that crocetin could effectively modulate antioxidant status in DENV-infected mice. Moreover, crocetin demonstrated the ability to reduce the nuclear translocation of NF-kB in DENV-infected mice. Our results suggest that crocetin treatment does not inhibit DENV replication in the liver of DENV-infected mice; however, we did find that crocetin improves host responses that reduce liver injury.
RT-dPCR in Mosquito Samples for ZIKV Detection: Effects of RNA Extraction and Reverse Transcription in Target Concentration
Authors: Almeida Paula Rodrigues de , Eisen Ana Karolina Antunes , Demoliner Meriane , Spilki Fernando Rosado
Published: July 2020

Zika virus (ZIKV) is an important arbovirus, responsible for recent outbreaks of Guillain Barré Syndrome and Congenital Zika Syndrome (CZS). After thousands of CZS cases, ZIKV is under constant surveillance in Brazil. Reliable and robust detection techniques are required to minimize the influence of host inhibitors from clinical samples and mosquito pool samples. Reverse transcription Digital Polymerase Chain Reaction (RT-dPCR) is a technique that allows the accurate quantification of DNA targets with high sensitivity, and it is usually less affected by inhibitors than RT-qPCR. This study aimed to assess the influence of mosquito tissue, RNA extraction and cDNA synthesis in ZIKV PCR detection. Samples containing 0, 3 and 10 mosquitoes were spiked with ZIKV MR766 and serially diluted prior to RNA extraction and RT-dPCR for ZIKV. Two reverse transcription protocols were tested. Assay sensitivity allowed the detection of 1.197 copies/µL. A higher correlation between dilution factor and target quantification was observed in 10 mosquito pool samples. The lower quantification in samples diluted without mosquitoes highlights the critical role of the reverse transcription step in RNA detection, since it could be attributed to reverse transcriptase variable performance in samples with low overall RNA concentration. The results in mosquito pools indicate that mosquito tissues do not inhibit ZIKV RT-dPCR, and the RT-dPCR technique has good sensitivity and robustness for ZIKV detection in mosquito pool samples regardless of mosquito tissue concentration.
Broadly Neutralizing Antibodies for Influenza: Passive Immunotherapy and Intranasal Vaccination
Authors: Biswas Mrityunjoy , Yamazaki Tatsuya , Chiba Joe , Akashi-Takamura Sachiko
Published: July 2020

Influenza viruses cause annual epidemics and occasional pandemics. The high diversity of viral envelope proteins permits viruses to escape host immunity. Therefore, the development of a universal vaccine and broadly neutralizing antibodies (bnAbs) is essential for controlling various mutant viruses. Here, we review some potentially valuable bnAbs for influenza; one is a novel passive immunotherapy using a variable domain of heavy chain-only antibody (VHH), and the other is polymeric immunoglobulin A (pIgA) induced by intranasal vaccination. Recently, it was reported that a tetravalent multidomain antibody (MDAb) was developed by genetic fusion of four VHHs, which are bnAbs against the influenza A or B viruses. The transfer of a gene encoding the MDAb–Fc fusion protein provided cross-protection against both influenza A and B viruses in vivo. An intranasal universal influenza vaccine, which can induce neutralizing pIgAs in the upper respiratory tract, is currently undergoing clinical studies. A recent study has revealed that tetrameric IgAs formed in nasal mucosa are more broadly protective against influenza than the monomeric and dimeric forms. These broadly neutralizing antibodies have high potential to control the currently circulating influenza virus.
Effects of Water Stress on Photosynthesis, Yield, and Water Use Efficiency in Winter Wheat
Authors: Zhao Wenhui , Liu Leizhen , Shen Qiu , Yang Jianhua , Han Xinyi , Tian Feng , Wu Jianjun
Published: July 2020

Drought has become one of the major constraints to agricultural development, particularly in areas that lack water. Studying the effects of different water stresses on the photosynthesis, growth, yield, water use efficiency (WUE) and irrigation water productivity (IWP) of winter wheat will provide data for the development of scientific irrigation strategies for water-saving agricultural methods. According to the size of the field water capacity, four different water stress levels were set, i.e., 30–40% (severe stress), 40–50% (moderate stress), 50–60% (mild stress) and 60–80% (well-watered) of field water capacity, controlling the amount of irrigation through an automatic irrigation system. The results showed that the seasonal changes in photosynthetic parameters, such as net photosynthetic rate (Pn), intercellular carbon concentration (Ci), stomatal conductance (Gs) and transpiration (E), significantly decreased under moderate and severe stress. As a result, the height, biomass and grain size of winter wheat decreased significantly, which led to low WUE and IWP. The Pn of the mild stress group only slightly decreased compared to that of the well-watered group, and was actually higher during the flowering and grain-filling stages, resulting in increases in dry biomass and 1000 grain weight of 2.07% and 1.95%, respectively. Higher WUE and IWP were attributed to higher yields and less water use. Thus, mild stress (60–80% field water capacity) resulted in the optimal use of water resources without a significant reduction in yield in the North China Plain (NCP). Therefore, mild stress can be considered a suitable environment for winter wheat growth in arid areas.
Detection of Groundwater Levels Trends Using Innovative Trend Analysis Method in Temperate Climatic Conditions
Authors: Minea Ionuț , Boicu Daniel , Chelariu Oana-Elena
Published: July 2020

The evolution of groundwater levels is difficult to predict over medium and long term in the context of global climate change. Innovative trend analysis method (ITA) was used to identify these trends, and ITA index was calculated to measure their magnitude. The data used are sourced from 71 hydrogeological wells that were dug between 1983 and 2018 and cover an area of over 8000 km2 developed in the temperate continental climate in the north-eastern part of Romania. The results obtained by applying the ITA show a general positive trend for groundwater level over 50% of wells for winter and spring seasons and annual values. The negative trends were observed for more than 43% of wells for the autumn season followed by the summer season (less than 40%). The magnitude of trends across the region shows a significant increase for spring season (0.742) followed by winter season (0.353). Important changes in the trends slopes and magnitudes have been identified for groundwater level depth between 0 and 4 m (for winter and spring seasons) and between 4 and 6 m (for summer and autumn seasons). The results can be implemented in groundwater resources management projects at local and regional level.
The Role of Experience and Different Sources of Knowledge in Shaping Flood Risk Awareness
Authors: Mondino Elena , Scolobig Anna , Borga Marco , Di Baldassarre Giuliano
Published: July 2020

Understanding what makes people vulnerable to flooding is key in informing the risk management process. Non-structural measures, such as risk communication, can reduce vulnerability by improving flood risk awareness, but they require a deep understanding of which factors influence risk awareness, and how. We analysed and untangled the role of experience with, and knowledge of, floods by conducting a survey in a municipality in North-eastern Italy that was hit by a flash flood in 2018. The results show that previous experience with floods influences risk awareness not only directly, but also indirectly through the knowledge that was gained from that experience. In addition, specific (as opposed to generic) definitions of experience have been found to be better suited for exploring their effects on risk awareness. Based on the literature and on our results, we propose an experience-knowledge typology to help unravel the complex role that these two variables play in shaping flood risk awareness.
Groundwater Flow-Modeling and Sensitivity Analysis in a Hyper Arid Region
Authors: Al-Muqdadi Sameh W. H. , Abo Rudy , Khattab Mohammed O. , Abdulhussein Firas M.
Published: July 2020

Groundwater modelling is particularly challenging in arid regions where limited water recharge is available. A fault zone will add a significant challenge to the modelling process. The Western Desert in Iraq has been chosen to implement the modelling concept and calculate the model sensitivity to the changes in aquifer hydraulic properties and calibration by researching 102 observations and irrigation wells. MODFLOW-NWT, which is a Newtonian formulation for MODFLOW-2005 approaches, have been used in this study. Further, the simulation run has been implemented using the Upstream-Weighting package (UPW) to treat the dry cells. The results show sensitivity to the change of the Kx value for the major groundwater discharge flow. Only about 7% of the models from the region can be irrigated utilizing greenhouses supported by external recharge.
Evaluating Mismatches between Legislation and Practice in Maintaining Environmental Flows
Authors: Chappell Jessica , German Laura , McKay S. Kyle , Pringle Cathy
Published: July 2020

Freshwater is essential to human communities and stream ecosystems, and governments strive to manage water to meet the needs of both people and ecosystems. Balancing competing water demands is challenging, as freshwater resources are limited and their availability varies through time and space. One approach to maintain this balance is to legally mandate that a specified amount of stream flow be maintained for stream ecosystems, known as an environmental flow. But laws and regulations do not necessarily reflect what happens in practice, potentially to the detriment of communities and natural systems. Through a case study of Puerto Rico, we investigated whether water management in practice matches legislative mandates and explored potential mismatch drivers. We focused on two governance targets—equitable allocation and water use efficiency—and assessed whether they are enshrined in the law (de jure) and how they manifest in practice (de facto). We also explored agency accountability through identifying agency structure and whether consequences are enforced for failing to carry out responsibilities. Our results indicate there are mismatches between how freshwater is governed by law and what occurs in practice. This study suggests that agency accountability may be necessary to consider when developing environmental flow legislation that will effectively achieve ecological outcomes.
Riverbed Changes of the Uppermost Atchafalaya River, USA—A Case Study of Channel Dynamics in Large Man-Controlled Alluvial River Confluences
Authors: Wang Bo , Xu Y. Jun , Xu Wei , Cheng Heqin , Chen Zhongyuan , Zhang Weiguo
Published: July 2020

River confluences are important nodes for downstream sediment transport and geomorphological development. Previous studies have established the knowledge that under natural conditions, river confluence zones experience channel scour followed with middle channel bar development. Less care is however given to the intensity of a confluence scour zone under man-controlled conditions, such as discharge regulation and levee confinement. In general, our knowledge about long-term bed evolution downstream of large alluvial river confluences is limited. Here we conducted a study focused on the 69-km uppermost channel of the Atchafalaya River, the largest distributary of the Mississippi River, to test the hypothesis that the channel downstream of two large tributaries sustains longer-term, extensive bed scouring owing to increased discharge in the main channel and, therefore, mid-channel bars in such a confluence zone cannot be built under confined channel conditions. The Atchafalaya River carries the total flow from the Red River and approximately 25% of the Mississippi River flow, traveling southwards 230 km before emptying into the Gulf of Mexico. We utilized long-term records on water surface elevation and discharge during 1935–2016, as well as channel bathymetry survey data during 1998–2006 to determine changes in hydraulic head and morphologic deformation in the confluence zone. The results from this study show that the combined flow from the Red River and Mississippi River into the Atchafalaya River steadily increased to approximately 5848 cubic meters per second (m3 s−1) in the recent decades, and the channel bed of the uppermost Atchafalaya River experienced considerable erosion since the 1930s. At a specific discharge of 8000 ± 100 m3 s−1, the river stage decreased by 5.8, 5.6, and 4.9 m from 1935 to 2016 at (from upstream to downstream) Simmesport, Melville, and Krotz Springs gauging stations, respectively. The average bed elevation reduced by 1.9 m from 1998 to 2006, although its thalweg increased by 0.3 m. Based on the channel bed assessment, a total volume of 6.6 × 107 m3 sediment was eroded from the uppermost 69 km of the Atchafalaya over the 8 years. The findings suggest that confluence zones of large alluvial rivers under controlled flow and confined levee conditions can experience extensive, long-lasting channel erosion. This can be especially progressive if the channel below a confluence is confined by levees, which can increase drag forces and prevent middle channel deposition. Further studies are needed to determine if the eroded sediment from the uppermost Atchafalaya is carried out to the river mouth or is deposited in the lower Atchafalaya. Such knowledge will have both scientific and practical relevance in river engineering and management.
Expert Knowledge and Perceptions about the Ecosystem Services and Natural Values of Hungarian Fishpond Systems
Authors: Palásti Péter , Kiss Márton , Gulyás Ágnes , Kerepeczki Éva
Published: July 2020

In the past few decades, multiple theoretical studies have highlighted the diverse capabilities of freshwater fishpond systems in the provision of water-related ecosystem services (ESs). However, practical studies to confirm this statement are still lacking in the scientific literature compared to other ecosystems. In this paper, we reveal the ESs of three semi-intensively managed fishpond systems in Hungary and assess the knowledge and perceptions of local experts about them and their main interactions. Between 2017 and 2019, we performed participatory research on the fishpond systems of Biharugra, Akasztó, and Szeged, conducting a total of 22 structured interviews with experts from all related stakeholder groups. Based on the interviews, we identified 16 actively used ESs (4 provisioning, 7 regulating, and 5 cultural ESs) and also revealed 19 main forms of impacts (14 positive and 5 negative) related to them. Despite their different perceptions and demands associated with fish farms, almost every expert articulated the role of semi-intensive fish farming in the sustenance of water-related natural values and ecosystem services, endangered by the ongoing effects of global warming. Besides confirming the theoretical statements of previous studies, these findings could also provide information for subsequent land-use planning, with the aim of creating more sustainable, multifunctionally used fishpond systems.
Spatio-Temporal Variation of Drought within the Vegetation Growing Season in North Hemisphere (1982–2015)
Authors: Zeng Zhaoqi , Li Yamei , Wu Wenxiang , Zhou Yang , Wang Xiaoyue , Huang Han , Li Zhaolei
Published: July 2020

Drought disasters jeopardize the production of vegetation and are expected to exert impacts on human well-being in the context of global climate change. However, spatiotemporal variations in drought characteristics (including the drought duration, intensity, and frequency), specifically for vegetation areas within a growing season, remain largely unknown. Here, we first constructed a normalized difference vegetation index to estimate the length of the growing season for each pixel (8 km) by four widely used phenology estimation methods; second, we analyzed the temporal and spatial patterns of climate factors and drought characteristics (in terms of the Standardized Precipitation Evapotranspiration Index (SPEI)), within a growing season over vegetation areas of the northern hemisphere before and after the critical time point of 1998, which was marked by the onset of a global warming hiatus. Finally, we extracted the highly drought-vulnerable areas of vegetation by examining the sensitivity of the gross primary production to the SPEI to explore the underlying effects of drought variation on vegetation. The results revealed, first, that significant (p < 0.05) increases in precipitation, temperature, and the SPEI (a wetting trend) occurred from 1982 to 2015. The growing season temperature increased even more statistically significant after 1998 than before. Second, the duration and frequency of droughts changed abruptly and decreased considerably from 1998 to 2015; and this wetting trend was located mainly in high-latitude areas. Third, at the biome level, the wetting areas occurred mainly in the tundra, boreal forest or taiga, and temperate coniferous forest biomes, whereas the highly drought-vulnerable areas were mainly located in the desert and xeric shrubland (43.5%) biomes. Our results highlight the fact that although the drought events within a growing season decreased significantly in the northern hemisphere from 1998 to 2015, the very existence of a mismatch between a reduction in drought areas and an increase in highly drought-vulnerable areas makes the impact of drought on vegetation nonnegligible. This work provides valuable information for designing coping measures to reduce the vegetative drought risk in the Northern Hemisphere.