bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-reported prevalence and potential factors influencing cardio-cerebral vascular disease among the Chinese elderly: A national cross-sectional study
Authors: Meng Lingbing , Xu Jiapei , Li Jianyi , Hu Jiabin , Xu Hongxuan , Wu Dishan , Hu Xing , Zeng Xuezhai , Zhang Qiuxia , Li Juan , Gong Tao , Liu Deping
Published: October 2022

Background

Aging is an essential national condition throughout China in the 21st century. Cardio-cerebral vascular disease (CCVD) is a common chronic vascular disease in the elderly. Despite aging becoming an increasingly pressing issue, there has been no comprehensive national investigation into the risk factors, prevalence, and management of CCVD among the elderly population in China.

Materials and methods

Through the 4th Survey of the Aged Population in Urban and Rural China (SSAPUR), a nationally representative sample of 224,142 adults aged more than 60 years was surveyed using a multistage, stratified sampling method. The 4th SSAPUR was used to investigate CCVD in the elderly. Univariate and multivariate logistic proportional regression analyses explored the risk factors. These risk factors were then entered into a multivariate linear regression model to identify independent predictive factors for CCVD. Disease management was assessed from the self-reported history of physician diagnosis, treatments, and hospital visits among individuals with CCVD.

Results

After excluding samples with missing information, 215,041 individuals were included in the analysis. The overall prevalence of CCVD was 26%. Living in a rural area, being older, being female, having low literacy, smoking, getting little sleep, losing a spouse, being single, not getting enough exercise, having a bad financial situation, and not taking part in public welfare programs were the main risk factors for CCVD among the elderly in China (P < 0.05). In the multivariate linear regression model, holding all other variables at any fixed value, CCVD remained associated with “urban and rural” (β = 0.012, P < 0.001), “age” (β = −0.003, P < 0.001), “sex” (β = −0.022, P < 0.001), “education level” (β = −0.017, P < 0.001), “marriage” (β = 0.004, P = 0.047), “smoking” (β = 0.012, P = 0.003), “drinking” (β = −0.015, P = 0.001), and “sleep” (β = 0.008, P = 0.005). There were no collinearity problems among these factors.

Conclusion

Major risk factors for prevalent CCVD among the elderly in China include the following: rural residence, female, low literacy level, poor sleep quality, bereavement, non-marriage, living alone, lack of exercise, poor financial situation, and non-participation in public welfare activities. Chinese national policies for preventing, controlling, and managing risk factors for CCVD in the elderly must be urgently developed.
Conversation difficulties rather than language deficits are linked to emotional problems in school children with hearing loss
Authors: Holzinger Daniel , Fellinger Johannes
Published: October 2022

Background

Social communication difficulties are often associated with neurodevelopmental disorders such as language disorder, intellectual development disorder, attention deficit hyperactivity disorder and autism spectrum disorder, which impedes the investigation of the specific role of social communication problems in mental health outcomes.

Aims

We investigated the association between conversation difficulties and increased risk of mental health problems in schoolchildren with hearing loss. It can be assumed of this sample that conversation difficulties are primarily due to limited auditory access to language rather than neurobiological deficits.

Methods

Five complete birth cohorts of Carinthian children (n = 53) with significant hearing loss and an intelligence quotient over 70 were assessed in terms of language abilities in standardized tests and in terms of their conversation skills by use of a short teacher checklist developed by the authors. Mental health problems were assessed by means of the Strengths and Difficulties Questionnaire by parents and teachers.

Results

Conversation deficits were significantly correlated with lower SDQ emotional problems and total problem scores as rated by parents and lower peer problems scores as rated by teachers. Controlling for degree of hearing loss, maternal education and language skills, confirmed the specific value of conversation skills as a predictor of emotional problems (p = 0.010).

Conclusion

With regard to mental health, our results suggest a focus on the assessment of conversation skills in addition to language abilities.
Ferroptosis: A potential target for the intervention of intervertebral disc degeneration
Authors: Zhou Lu-Ping , Zhang Ren-Jie , Jia Chong-Yu , Kang Liang , Zhang Zhi-Gang , Zhang Hua-Qing , Wang Jia-Qi , Zhang Bo , Shen Cai-Liang
Published: October 2022

Ferroptosis, an iron-dependent form of programmed cell death marked by phospholipid peroxidation, is regulated by complex cellular metabolic pathways including lipid metabolism, iron balance, redox homeostasis, and mitochondrial activity. Initial research regarding the mechanism of ferroptosis mainly focused on the solute carrier family 7 member 11/glutathione/glutathione peroxidase 4 (GPX4) signal pathway. Recently, novel mechanisms of ferroptosis, independent of GPX4, have been discovered. Numerous pathologies associated with extensive lipid peroxidation, such as drug-resistant cancers, ischemic organ injuries, and neurodegenerative diseases, are driven by ferroptosis. Ferroptosis is a new therapeutic target for the intervention of IVDD. The role of ferroptosis in the modulation of intervertebral disc degeneration (IVDD) is a significant topic of interest. This is a novel research topic, and research on the mechanisms of IVDD and ferroptosis is ongoing. Herein, we aim to review and discuss the literature to explore the mechanisms of ferroptosis, the relationship between IVDD and ferroptosis, and the regulatory networks in the cells of the nucleus pulposus, annulus fibrosus, and cartilage endplate to provide references for future basic research and clinical translation for IVDD treatment.
Association between hyperuricemia and metabolic syndrome: A cross-sectional study in Tibetan adults on the Tibetan plateau
Authors: Yao Shaoli , Zhou Yao , Xu Li , Zhang Qi , Bao Shimin , Feng Huiru , Ge Weihong
Published: October 2022

Purpose

This study aimed to assess the relationship of serum uric acid with metabolic syndrome and its components in Tibetan adults on the Tibetan plateau.

Methods

A total of 307 participants were enrolled in this study and biochemical parameters including serum uric acid, fasting plasma glucose, white blood cell, lymphocyte count, mononuclear cells, alanine aminotransferase, aspartate aminotransferase, creatinine, and lipid profile were analyzed using standard methods. The IDF criteria were applied to define metabolic syndrome. The association of serum uric acid with metabolic syndrome and its components was evaluated by multivariable logistic regression models.

Results

The overall prevalence of metabolic syndrome was 17.3% (53/307) with 19.6% (31/158) in females and 14.8% (22/149) in male participants. The prevalence of hyperuricemia was 40.7% (125/307) with significant differences between the male (53.7%,80/149) and female (28.5%,45/158) groups. In regression analysis, we observed that the risk of MetS was higher in participants in the hyperuricemia group (adjusted OR, 4.01; 95% CI, 2.02~7.99) compared with those in the normouricemia group. After adjusting for all confounding factors, a 9% higher risk of MetS could be shown in participants with SUA increased per 10umol/L (adjusted OR, 1.09; 95% CI, 1.04~1.14). These relationships were not affected by sex or age (p >0.05). After adjusting for the confounding factors, hyperuricemia is positively associated with abdominal obesity (adjusted OR, 2.53; 95% CI, 1.41~4.53), elevated blood pressure (adjusted OR, 2.61; 95% CI, 1.37~4.97), and elevated triglycerides(adjusted OR, 2.47; 95% CI, 1.09~5.57).

Conclusions

In our study, hyperuricemia is significantly associated with the prevalence of metabolic syndrome and part of its components, and these relationships are not affected by sex or age. Given the high prevalence of MetS and hyperuricemia among Tibetan adults, more studies are required to explore the role of SUA in the pathogenesis of MetS.
Can China’s digital inclusive finance help rural revitalization? A perspective based on rural economic development and income disparity
Authors: Xiong Mingzhao , Fan Jingjing , Li Wenqi , Sheng Xian Brian Teo
Published: October 2022

The development of digital inclusive finance in China can help alleviate the problems of the “three rural areas” and contribute to rural revitalization. The impact of digital inclusive finance on rural revitalization is examined by multiple linear regression, mediated effect model, and threshold effect model in a sample of 30 provinces in China from 2011 to 2020. It is found that (1) the digital inclusive finance index and its sub-indicators - the breadth of digital financial coverage and depth of digital financial use - can promote rural revitalization in China, but the digitalization degree of its sub-indicators does not have a significant linear effect on rural revitalization, but a “U” shaped relationship of first inhibiting and then promoting, with 2017 as the boundary. (2) The regional heterogeneity test reveals that the promotion effect of the digital financial inclusion index and its sub-indicators of digital financial coverage breadth and digital financial usage depth on rural revitalization is more significant in the eastern coastal and central inland provinces, and insignificant in the western remote areas. (3) The mediating effect test finds that digital inclusive finance in China can contribute to rural revitalization by promoting economic growth in rural areas and narrowing the income gap between urban and rural areas. (4) The threshold effect test reveals that there is a single threshold effect of digital inclusive finance and its three sub-indicators on rural revitalization in China. When the digital inclusive finance index and the three sub-dimensions are below the threshold value, they have a facilitating effect on rural revitalization, and after crossing the threshold value, their facilitating effect on rural revitalization becomes more significant. Based on the empirical analysis results, this paper puts forward suggestions for improving the development mechanism of digital inclusive finance, formulating differentiated financial support policies and strengthening financial supervision according to regional differences, and establishing a long-term mechanism for rural revitalization, to promote China’s rural revitalization.
Participatory management of weed infestation in Lake Tana - setting a better future in the front mirror
Authors: Sewunet Birhanu , Girma Haileyesus , Debela Sisay Abebe , Luke Amana Ogeto , Gebrehiwot Mesfin
Published: October 2022

Lake Tana is one of the biosphere reserves in Ethiopia registered by the United Nations Educational, Scientific, and Cultural Organization (UNESCO). Water hyacinth is expanding in the lake owing to increased nutrient load from the watershed. As efforts from the government alone could not bring the anticipated change, local peoples’ engagement and contribution are believed to sustainably and effectively manage the watershed and prevent the weed’s spreading. This study, therefore, aims to estimate households’ contribution (willingness to pay–WTP–and willingness to contribute labor–WTCL–) to prevent and control water hyacinth and hence improve lake ecosystem services through a hypothetical watershed management in Lake Tana. This study applied quantitative and qualitative methods. It was conducted among 560 randomly selected households of the Libo-kemkem district using a contingent valuation method from March to April/2021. Eleven key informant interviews were also conducted. Bivariable and multivariable interval regression models were used to identify the determinants of households’ potential contribution. Thematic analysis was used to analyze the qualitative data. As the weed is believed negatively impact the ecosystem services provided by the lake, 72% of the respondents showed willingness to contribute to the proposed management program. About 32 and 39% of the respondents were willing to pay in cash and to contribute labor, respectively, to prevent weed infestation. On the other hand, 28% of them were neither willing to contribute in cash nor in labor to the hypothetical market. The findings showed respondents’ mean annual WTP and WTCL were 632.8 Ethiopian birr and 30.1 man-days per household, respectively. The place of residence, educational status, farm plot area, household income, family size, and conference participation significantly influenced WTP and/or WTCL. In this hypothetical improvement scenario, an estimated annual WTP and WTCL of nearly 12 million Ethiopian birr and 700 man-days per year, respectively, are reported considering the total households in the study area. Therefore, collaborative efforts by different stakeholders are the next steps to realize the hypothetical contributions.
Functional anatomy and disparity of the postcranial skeleton of African mole-rats (Bathyergidae)
Authors: Montoya-Sanhueza Germán , Bennett Nigel C. , Chinsamy Anusuya , Šumbera Radim
Published: October 2022

The burrowing adaptations of the appendicular system of African mole-rats (Bathyergidae) have been comparatively less investigated than their cranial adaptations. Because bathyergids exhibit different digging modes (scratch-digging and chisel-tooth digging) and social systems (from solitary to highly social), they are a unique group to assess the effects of distinct biomechanical regimes and social organization on morphology. We investigated the morphological diversity and intraspecific variation of the appendicular system of a large dataset of mole-rats (n = 244) including seven species and all six bathyergid genera. Seventeen morpho-functional indices from stylopodial (femur, humerus) and zeugopodial (ulna, tibia-fibula) elements were analyzed with multivariate analysis. We hypothesized that scratch-diggers (i.e., Bathyergus) would exhibit a more specialized skeletal phenotype favoring powerful forelimb digging as compared to the chisel-tooth diggers, and that among chisel-tooth diggers, the social taxa will exhibit decreased limb bone specializations as compared to solitary taxa due to colony members sharing the costs of digging. Our results show that most bathyergids have highly specialized fossorial traits, although such specializations were not more developed in Bathyergus (or solitary species), as predicted. Most chisel tooth-diggers are equally, or more specialized than scratch-diggers. Heterocephalus glaber contrasted significantly from other bathyergids, presenting a surprisingly less specialized fossorial morphology. Our data suggests that despite our expectations, chisel-tooth diggers have a suite of appendicular adaptations that have allowed them to maximize different aspects of burrowing, including shoulder and neck support for forward force production, transport and removal of soils out of the burrow, and bidirectional locomotion. It is probably that both postcranial and cranial adaptations in bathyergids have played an important role in the successful colonization of a wide range of habitats and soil conditions within their present distribution.
Abrogation of neutrophil inflammatory pathways and potential reduction of neutrophil-related factors in COVID-19 by intravenous immunoglobulin
Authors: Masso-Silva Jorge Adrian , Sakoulas George , Olay Jarod , Groysberg Victoria , Geriak Matthew , Nizet Victor , Crotty Alexander Laura E. , Meier Angela
Published: October 2022

Pathogenesis of lung injury in COVID-19 is not completely understood, leaving gaps in understanding how current treatments modulate the course of COVID-19. Neutrophil numbers and activation state in circulation have been found to correlate with COVID-19 severity, and neutrophil extracellular traps (NETs) have been found in the lung parenchyma of patients with acute respiratory distress syndrome (ARDS) in COVID-19. Targeting the pro-inflammatory functions of neutrophils may diminish lung injury in COVID-19 and ARDS. Neutrophils were isolated from peripheral blood of healthy donors, treated ex vivo with dexamethasone, tocilizumab and intravenous immunoglobulin (IVIG) and NET formation, oxidative burst, and phagocytosis were assessed. Plasma from critically ill COVID-19 patients before and after clinical treatment with IVIG and from healthy donors was assessed for neutrophil activation-related proteins. While dexamethasone and tocilizumab did not affect PMA- and nigericin-induced NET production ex vivo, IVIG induced a dose-dependent abrogation of NET production in both activation models. IVIG also reduced PMA-elicited reactive oxygen species production, but did not alter phagocytosis. COVID-19 patients were found to have elevated levels of cell-free DNA, neutrophil elastase and IL-8 as compared to healthy controls. Levels of both cell-free DNA and neutrophil elastase were lower 5 days after 4 days of daily treatment with IVIG. The lack of impact of dexamethasone or tocilizumab on these neutrophil functions suggests that these therapeutic agents may not act through suppression of neutrophil functions, indicating that the door might still be open for the addition of a neutrophil modulator to the COVID-19 therapeutic repertoire.
Photoperiod-independent diurnal feeding improved the growth and feed utilization of juvenile rainbow trout (Oncorhynchus mykiss) by inducing food anticipatory activity
Authors: Xu Hanying , Shi Ce , Ye Yangfang , Mu Changkao , Wang Chunlin
Published: October 2022

A three-month culture experiment was designed to assess the effects of photoperiod and feeding regime on growth, feed utilization, and food anticipatory activity (FAA) of juvenile rainbow trout. The experiment included two photoperiods: 24L:0D (LL) and 12L:12D (LD); three feeding regimes: random feeding (R), mid-dark stage feeding (D), and mid-light stage feeding (L). A total of six treatment groups (R-LL, D-LL, L-LL, R-LD, D-LD, L-LD) were defined. The experimental results showed that the growth and feed utilization of the scheduled feeding groups (D and L groups) were significantly higher than those of the R group under both photoperiods, and there was no statistical difference between the D and L groups. A typical FAA was observed in the L group, independent of the photoperiod. Also, the digestive enzyme activity of the L group was synchronized by the feeding time under both photoperiods. There were rhythms in serum levels of glucose (GLU), triglyceride (TG), and total-cholesterol (T-CHO) in the D and L groups. Serum GLU also had a rhythm in the R group, but the peaks occurred at the feeding point (LL group) or after the feeding point (LD group), reflecting a possible passive rise in GLU after feeding. Serum cortisol was higher in the R group than in the scheduled feeding group, indicating that random feeding caused stress to juvenile rainbow trout. Serum insulin levels were found to increase before feeding in all three feeding regimes, probably reflecting the anticipation of food induced by the last meal. Serum melatonin levels were suppressed by the LL group. Serum 5-HT levels were synchronized by meal time in the R and L groups. Finally, rhythms of appetite-related genes were observed under all three feeding regimes, and more genes were rhythmic under LL, suggesting that food can strongly synchronize the feeding rhythm of juvenile rainbow trout when lacking light zeitgeber. In summary, this study concluded that diurnal feeding (L group) independent of photoperiod induced typical FAA in juvenile rainbow trout and that the LD (12L:12D) photoperiod and L (mid-light phase) feeding were recommended in the juvenile rainbow trout aquaculture.
Fecal microbiota transplantation in patients with post-infectious irritable bowel syndrome: A randomized, clinical trial
Authors: Tkach Sergii , Dorofeyev Andrii , Kuzenko Iurii , Sulaieva Oksana , Falalyeyeva Tetyana , Kobyliak Nazarii
Published: October 2022

Introduction

Research in recent years has shown the potential benefits of fecal microbiota transplantation (FMT) for irritable bowel syndrome (IBS). Acute infectious gastroenteritis is a well-established risk factor for developing such forms of IBS as post-infectious IBS (PI-IBS). However, the effective use of FMT in patients with IP-IBS has not yet been clarified.

Aim

The study aimed to conduct a single-center, randomized clinical trial (RCT) to assess FMT’s safety, clinical and microbiological efficacy in patients with PI-IBS.

Materials and methods

Patients with PI-IBS were randomized into two groups: I (standard-care, n = 29) were prescribed basic therapy, namely a low FODMAP diet, as well as Otilonium Bromide (1 tablet TID) and a multi-strain probiotic (1 capsule BID) for 1 month; II (FMT group, n = 30), each patient with PI-IBS underwent a single FMT procedure with fresh material by colonoscopy. All patients underwent bacteriological examination of feces for quantitative and qualitative microbiota composition changes. The clinical efficacy of treatment was evaluated according to the dynamics of abdominal symptoms, measured using the IBS-SSS scale, fatigue reduction (FAS scale), and a change in the quality of life (IBS-QoL scale).

Results

FMT was associated with rapid onset of the effect, manifested in a significant difference between IBS-SSS points after 2 weeks of intervention (p < 0.001). In other time points (after 4 and 12 weeks) IBS-SSS did not differ significantly across both groups. Only after 3 months of treatment did their QoL exceed its initial level, as well value for 2 and 4 weeks, to a significant extent. The change in the ratio of the main microbial phenotypes in the form of an increase in the relative abundance of Firmicutes and Bacteroidetes was recorded in all patients after 4 weeks. It should be noted that these changes were significant but eventually normalized only in the group of PI-IBS patients who underwent FMT. No serious adverse reactions were noted.

Conclusion

This comparative study of the results of FMT use in patients with PI-IBS demonstrated its effectiveness compared to traditional pharmacotherapy, as well as a high degree of safety and good tolerability.
Unraveling the mechanism of furfural tolerance in engineered Pseudomonas putida by genomics
Authors: Zou Lihua , Jin Xinzhu , Tao Yuanming , Zheng Zhaojuan , Ouyang Jia
Published: October 2022

As a dehydration product of pentoses in hemicellulose sugar streams derived from lignocellulosic biomass, furfural is a prevalent inhibitor in the efficient microbial conversion process. To solve this obstacle, exploiting a biorefinery strain with remarkable furfural tolerance capability is essential. Pseudomonas putida KT2440 (P. putida) has served as a valuable bacterial chassis for biomass biorefinery. Here, a high-concentration furfural-tolerant P. putida strain was developed via adaptive laboratory evolution (ALE). The ALE resulted in a previously engineered P. putida strain with substantially increased furfural tolerance as compared to wild-type. Whole-genome sequencing of the adapted strains and reverse engineering validation of key targets revealed for the first time that several genes and their mutations, especially for PP_RS19785 and PP_RS18130 [encoding ATP-binding cassette (ABC) transporters] as well as PP_RS20740 (encoding a hypothetical protein), play pivotal roles in the furfural tolerance and conversion of this bacterium. Finally, strains overexpressing these three striking mutations grew well in highly toxic lignocellulosic hydrolysate, with cell biomass around 9-, 3.6-, and two-fold improvement over the control strain, respectively. To our knowledge, this study first unravels the furan aldehydes tolerance mechanism of industrial workhorse P. putida, which provides a new foundation for engineering strains to enhance furfural tolerance and further facilitate the valorization of lignocellulosic biomass.
Vaginal microbiota networks as a mechanistic predictor of aerobic vaginitis
Authors: Wang Qian , Dong Ang , Zhao Jinshuai , Wang Chen , Griffin Christipher , Gragnoli Claudia , Xue Fengxia , Wu Rongling
Published: October 2022

Aerobic vaginitis (AV) is a complex vaginal dysbiosis that is thought to be caused by the micro-ecological change of the vaginal microbiota. While most studies have focused on how changes in the abundance of individual microbes are associated with the emergence of AV, we still do not have a complete mechanistic atlas of the microbe-AV link. Network modeling is central to understanding the structure and function of any microbial community assembly. By encapsulating the abundance of microbes as nodes and ecological interactions among microbes as edges, microbial networks can reveal how each microbe functions and how one microbe cooperate or compete with other microbes to mediate the dynamics of microbial communities. However, existing approaches can only estimate either the strength of microbe-microbe link or the direction of this link, failing to capture full topological characteristics of a network, especially from high-dimensional microbial data. We combine allometry scaling law and evolutionary game theory to derive a functional graph theory that can characterize bidirectional, signed, and weighted interaction networks from any data domain. We apply our theory to characterize the causal interdependence between microbial interactions and AV. From functional networks arising from different functional modules, we find that, as the only favorable genus from Firmicutes among all identified genera, the role of Lactobacillus in maintaining vaginal microbial symbiosis is enabled by upregulation from other microbes, rather than through any intrinsic capacity. Among Lactobacillus species, the proportion of L. crispatus to L. iners is positively associated with more healthy acid vaginal ecosystems. In a less healthy alkaline ecosystem, L. crispatus establishes a contradictory relationship with other microbes, leading to population decrease relative to L. iners. We identify topological changes of vaginal microbiota networks when the menstrual cycle of women changes from the follicular to luteal phases. Our network tool provides a mechanistic approach to disentangle the internal workings of the microbiota assembly and predict its causal relationships with human diseases including AV.
Sleep loss suicidal ideation: the role of trait extraversion
Authors: Killgore William D. S. , Grandner Michael A. , Tubbs Andrew S. , Fernandez Fabian-Xosé , Doty Tracy Jill , Capaldi II Vincent F. , Dailey Natalie S.
Published: October 2022

Background: It is known that sleep disturbance is associated with increased suicidal thinking. Moreover, completed suicides, when adjusted for the proportion of the populace that is awake at a given time, are more probable during the late night/early morning hours. Despite these concerns, no studies have examined the role of trait-like individual differences in vulnerability to suicidal ideation during sleep deprivation or insomnia. In two separate studies, we examined whether the trait of extraversion is predictive of changes in suicidal thinking following two nights of sleep deprivation and among individuals meeting the criteria for insomnia.

Methods:Study 1: Twenty-five healthy military personnel (20 males), ages 20–35 completed the NEO-PI-R Extraversion scale and the Suicidal Ideation (SUI) scale of the Personality Assessment Inventory (PAI). Participants completed 77 h of continuous sleep deprivation. After 56 h of sleep deprivation, participants completed the SUI scale a second time. We predicted a change in SUI scores from baseline extraversion. Study 2: 2,061 adults aged 18–79 (900 males) were divided into two groups based on the clinical threshold (≥ 10) on the Insomnia Severity Index (ISI) and completed measures of extraversion and depression, including the suicide item of the Patient Health Questionnaire-9 (PHQ9).

Results:Study 1: After controlling for the caffeine group and changes in PAI Depression, Extraversion scores were used to predict changes in SUI scores using stepwise multiple linear regression. Higher Extraversion was significantly associated with increased non-clinical suicidal ideation following sleep loss, β = 0.463, partial r = 0.512, p = 0.013. Study 2: After controlling for depression, the effect of insomnia on suicidal ideation was moderated by trait extraversion (p < 0.0001). Overall, the presence or absence of insomnia had little effect on individuals low in trait extraversion (i.e., introverts), but insomnia was associated with significantly higher suicidal ideation among high trait extraverted individuals.

Conclusions: Higher trait extraversion was associated with increased vulnerability to suicidal ideation between rested baseline and total sleep deprivation and was associated with greater suicidal ideation among those meeting criteria for clinically severe insomnia. These findings point to a potential trait-like vulnerability factor that may further our understanding of sleep disruption in the phenomenology of suicide.
A glial perspective on the extracellular matrix and perineuronal net remodeling in the central nervous system
Authors: Tewari Bhanu P. , Chaunsali Lata , Prim Courtney E. , Sontheimer Harald
Published: October 2022

A structural scaffold embedding brain cells and vasculature is known as extracellular matrix (ECM). The physical appearance of ECM in the central nervous system (CNS) ranges from a diffused, homogeneous, amorphous, and nearly omnipresent matrix to highly organized distinct morphologies such as basement membranes and perineuronal nets (PNNs). ECM changes its composition and organization during development, adulthood, aging, and in several CNS pathologies. This spatiotemporal dynamic nature of the ECM and PNNs brings a unique versatility to their functions spanning from neurogenesis, cell migration and differentiation, axonal growth, and pathfinding cues, etc., in the developing brain, to stabilizing synapses, neuromodulation, and being an active partner of tetrapartite synapses in the adult brain. The malleability of ECM and PNNs is governed by both intrinsic and extrinsic factors. Glial cells are among the major extrinsic factors that facilitate the remodeling of ECM and PNN, thereby acting as key regulators of diverse functions of ECM and PNN in health and diseases. In this review, we discuss recent advances in our understanding of PNNs and how glial cells are central to ECM and PNN remodeling in normal and pathological states of the CNS.
The role of butyrylcholinesterase in the regulation of cognitive dysfunction in minimal hepatic encephalopathy: A potential blood marker of disease evolution
Authors: Yang Xuhong , Dang Pei , Liu Wenxiao , Ma Wanlong , Ge Xin , Zhu Kai , Wang Minglei , Huang Xueying , Ding Xiangchun , Wang Xiaodong
Published: October 2022

Background and aims

Patients with cirrhosis commonly experience minimal hepatic encephalopathy (MHE), and alterations in neurotransmitters have been thought to be related to cognitive function. However, the relationship between alterations in peripheral and central butyrylcholinesterase (BuChE) with MHE disease progression remains unknown. As such, this study was designed to investigate potential changes in peripheral and central BuChE activity and their effects on cognitive function in the context of MHE.

Materials and methods

We enrolled 43 patients with cirrhosis secondary to hepatitis B, 20 without MHE and 23 with MHE, and 25 with healthy controls (HC). All the selected subjects underwent resting-state functional MRI, and the original images were processed to obtain the regional homogeneity (ReHo) brain maps. Thereafter, the correlation of BuChE activity with ReHo, number connection test of type A (NCT-A), and digital symbol test (DST) scores with MHE patients were analyzed using Person correlation analysis. Meanwhile, we purchased 12 Sprague-Dawley (SD) rats and divided them into an experimental group (n = 6) and a control group (n = 6). The rats in the experimental group were intraperitoneally injected with thioacetamide (TAA) to prepare MHE model rats. After modeling, we used the Morris water maze (MWM) and elevated plus maze (EPM) to assess the cognition function and exploratory behavior of all rats. The activity of serum, hippocampus, and frontal lobe tissue BuChE was detected by ELISA.

Results

BuChE activity gradually decreased among the HC, patients with cirrhosis, and MHE groups (all P < 0.01). We observed a linear correlation between serum BuChE and NCT-A and DST scores in MHE patients (all P < 0.01). We noted that BuChE activity can negatively correlate with ReHo values in the left middle temporal gyrus and left inferior temporal gyrus, and positively correlate with ReHo values in the right inferior frontal gyrus, and also found that the peripheral BuChE activity of MHE rats was significantly lower than their control counterparts, and the BuChE activity in frontal lobe extracts was significantly higher than the control rats (all P < 0.05).

Conclusion

The altered activity of BuChE may contribute to cognitive impairment in MHE patients, which may be a potential biomarker of disease evolution in the context of MHE.
Neural substrates of spatial processing and navigation in blindness: An activation likelihood estimation meta-analysis
Authors: Bleau Maxime , Paré Samuel , Chebat Daniel-Robert , Kupers Ron , Nemargut Joseph Paul , Ptito Maurice
Published: October 2022

Even though vision is considered the best suited sensory modality to acquire spatial information, blind individuals can form spatial representations to navigate and orient themselves efficiently in space. Consequently, many studies support theamodality hypothesisof spatial representations since sensory modalities other than vision contribute to the formation of spatial representations, independently of visual experience and imagery. However, given the high variability in abilities and deficits observed in blind populations, a clear consensus about the neural representations of space has yet to be established. To this end, we performed a meta-analysis of the literature on the neural correlates of spatial processing and navigation via sensory modalities other than vision, like touch and audition, in individuals with early and late onset blindness. An activation likelihood estimation (ALE) analysis of the neuroimaging literature revealed that early blind individuals and sighted controls activate the same neural networks in the processing of non-visual spatial information and navigation, including the posterior parietal cortex, frontal eye fields, insula, and the hippocampal complex. Furthermore, blind individuals also recruit primary and associative occipital areas involved in visuo-spatial processing via cross-modal plasticity mechanisms. The scarcity of studies involving late blind individuals did not allow us to establish a clear consensus about the neural substrates of spatial representations in this specific population. In conclusion, the results of our analysis on neuroimaging studies involving early blind individuals support theamodality hypothesisof spatial representations.
A two-stage hybrid gene selection algorithm combined with machine learning models to predict the rupture status in intracranial aneurysms
Authors: Li Qingqing , Wang Peipei , Yuan Jinlong , Zhou Yunfeng , Mei Yaxin , Ye Mingquan
Published: October 2022

An IA is an abnormal swelling of cerebral vessels, and a subset of these IAs can rupture causing aneurysmal subarachnoid hemorrhage (aSAH), often resulting in death or severe disability. Few studies have used an appropriate method of feature selection combined with machine learning by analyzing transcriptomic sequencing data to identify new molecular biomarkers. Following gene ontology (GO) and enrichment analysis, we found that the distinct status of IAs could lead to differential innate immune responses using all 913 differentially expressed genes, and considering that there are numerous irrelevant and redundant genes, we propose a mixed filter- and wrapper-based feature selection. First, we used the Fast Correlation-Based Filter (FCBF) algorithm to filter a large number of irrelevant and redundant genes in the raw dataset, and then used the wrapper feature selection method based on the he Multi-layer Perceptron (MLP) neural network and the Particle Swarm Optimization (PSO), accuracy (ACC) and mean square error (MSE) were then used as the evaluation criteria. Finally, we constructed a novel 10-gene signature (YIPF1, RAB32, WDR62, ANPEP, LRRCC1, AADAC, GZMK, WBP2NL, PBX1, and TOR1B) by the proposed two-stage hybrid algorithm FCBF-MLP-PSO and used different machine learning models to predict the rupture status in IAs. The highest ACC value increased from 0.817 to 0.919 (12.5% increase), the highest area under ROC curve (AUC) value increased from 0.87 to 0.94 (8.0% increase), and all evaluation metrics improved by approximately 10% after being processed by our proposed gene selection algorithm. Therefore, these 10 informative genes used to predict rupture status of IAs can be used as complements to imaging examinations in the clinic, meanwhile, this selected gene signature also provides new targets and approaches for the treatment of ruptured IAs.
Effect of treatment zone decentration on axial length growth after orthokeratology
Authors: Zhang Shuxian , Zhang Hui , Li Lihua , Yang Xiaoyan , Li Shumao , Li Xuan
Published: October 2022

Objective

To study the effect of treatment zone (TZ) decentration on axial length growth (ALG) in adolescents after wearing the orthokeratology lenses (OK lenses).

Materials and methods

This retrospective clinical study selected 251 adolescents who were fitted OK lenses at the Clinical College of Ophthalmology, Tianjin Medical University (Tianjin, China) from January 2018–December 2018 and wore them continuously for >12 months. The age of the subjects was 8–15 years, spherical equivalent (SE): −1.00 to −5.00 diopter (D), and astigmatism ≤ 1.50 D. The corneal topography were recorded at baseline and 1-, 6-, and 12-month visits, and the axial length (AL) were recorded at baseline and 6-, 12-month visits. The data of the right eye were collected for statistical analysis.

Results

The subjects were divided into three groups according to the decentration distance of the TZ after wearing lenses for 1 month: 56 cases in the mild (<0.5 mm), 110 in the moderate (0.5–1.0 mm), and 85 in the severe decentration group (>1.0 mm). A significant difference was detected in the ALG between the three groups after wearing lenses for 6 and 12 months (F = 10.223, P < 0.001; F = 13.380, P < 0.001, respectively). Among these, the 6- and 12-month ALG of the mild decentration group was significantly higher than that of the other two groups. Multivariable linear regression analysis showed that age, baseline SE, and 1-month decentration distance associated with the 12-month ALG (P < 0.001, P < 0.001, and P = 0.001, respectively).

Conclusion

The decentration of the TZ of the OK lens affected the growth of the AL in adolescents, i.e., the greater the decentration, the slower the ALG.
Butyrate: Connecting the gut-lung axis to the management of pulmonary disorders
Authors: Corrêa Renan Oliveira , Castro Pollyana Ribeiro , Moser René , Ferreira Caroline Marcantonio , Quesniaux Valerie F. J. , Vinolo Marco Aurélio Ramirez , Ryffel Bernhard
Published: October 2022

Short-chain fatty acids (SCFAs) are metabolites released by bacterial components of the microbiota. These molecules have a wide range of effects in the microbiota itself, but also in host cells in which they are known for contributing to the regulation of cell metabolism, barrier function, and immunological responses. Recent studies indicate that these molecules are important players in the gut-lung axis and highlight the possibility of using strategies that alter their intestinal production to prevent or treat distinct lung inflammatory diseases. Here, we review the effects of the SCFA butyrate and its derivatives in vitro and in vivo on murine models of respiratory disorders, besides discussing the potential therapeutic use of butyrate and the other SCFAs in lung diseases.
Age and cohort trends of the impact of socioeconomic status on dietary diversity among Chinese older adults from the perspective of urban–rural differences: A prospective cohort study based on CLHLS 2002–2018
Authors: Yu Yan , Cao Na , He Anqi , Jiang Junfeng
Published: October 2022

The association between socioeconomic status (SES) and dietary diversity score (DDS) has been widely discussed, but little is known about the age and cohort effects on DDS and how the SES effect on DDS varies with age and across successive cohorts among urban and rural older adults in China. Thus, this study aimed to examine the temporal change in DDS among Chinese older adults and SES heterogeneities in such change from the perspective of urban–rural differentiation. Data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) between 2002 and 2018 were used, and a total of 13,728 participants aged between 65 and 105 years were included in this study. A total of eight food groups were used to assess DDS, while education, family income, and perceived income status were used to assess SES. A linear mixed model was used to estimate the age and cohort effects on DDS and their urban–rural and SES disparities. The results show that higher SES, including more education, family income, and perceived income status, was associated with higher DDS (for urban older adults, β = 0.1645, p = 0.0003, β = 0.2638, p < 0.0001, β = 0.2917, p < 0.0001, respectively; for rural older adults, β = 0.0932, p = 0.0080, β = 0.4063, p < 0.0001, β = 0.2921, p < 0.0001, respectively). The DDS of older adults increased with age and across successive cohorts in both urban and rural China. Moreover, we found the three-way interaction effect of SES, age, and cohort was statistically significant in both urban and rural China. Thus, living in an urban area and having higher SES are associated with higher DDS, but these associations change with age and across successive cohorts. The dietary health of earlier cohorts and rural oldest-old in China deserves more attention.
A damage-associated molecular patterns-related gene signature for the prediction of prognosis and immune microenvironment in children stage III acute lymphoblastic leukemia
Authors: Zhao Feng , Lin Qiuyu , Xiang Xiayu , Xiang Wei
Published: October 2022

Background

Immunogenic cell death (ICD)-mediated immune response provides a strong rationale to overcome immune evasion in acute lymphoblastic leukemia (ALL). ICD will produce damage-associated molecular patterns (DAMPs) in tumor microenvironment. However, there are few studies on the application of DAMPs-related molecular subtypes in clinically predicting stage III of ALL prognosis. The current study is to identify the DAMPs-associated genes and their molecular subtypes in the stage III of ALL and construct a reliable risk model for prognosis as well as exploring the potential immune-related mechanism.

Materials and methods

We used Target and EBI database for differentially expressed genes (DEGs) analysis of the stage III pediatric ALL samples. Three clusters were identified based on a consistent clustering analysis. By using Cox regression and LASSO analysis, we determined DEGs that attribute to survival benefit. In addition, the Gene Set Enrichment Analysis (GSEA) was performed to identify potential molecular pathways regulated by the DAMPs-related gene signatures. ESTIMATE was employed for evaluating the composition of immune cell populations.

Results

A sum of 146 DAMPs-associated DEGs in ALL were determined and seven transcripts among them were selected to establish a risk model. The DAMPs-associated gene signature significantly contributed to worse prognosis in the high-risk group. We also found that the high-risk group exhibited low immune cell infiltration and high expression of immune checkpoints.

Conclusion

In summary, our study showed that the DAMPs-related DEGs in the stage III of children ALL could be used to predict their prognosis. The risk model of DAMPs we established may be more sensitive to immunotherapy prediction.
Huoxue Qingre decoction used for treatment of coronary heart disease network analysis and metabolomic evaluation
Authors: Tan Yu-Qing , Jin Min , He Xuan-Hui , Chen Heng-Wen
Published: October 2022

Objective: Network pharmacology provides new methods and references for the research of traditional Chinese medicine, but some problems remain, such as single evaluation components and index methods, imperfect relevant databases, unscientific prediction results, and lack of verification of results. Herein, we used a modified network pharmacology research method to explore the potential network analysis mechanism of Huoxue Qingre decoction in the treatment of coronary heart disease and utilized clinical trials for assessment.

Methods: Based on literature research, the targets corresponding to the drug were obtained with the assistance of the TCMSP database and Swiss Target Prediction, and the target proteins were corrected using the UniProt database. The targets related to coronary heart disease was obtained through the GeneCards database. A protein-protein interaction network diagram was constructed, and a “component-intersection target” network diagram was drawn based on Cytoscape 3.6.2 software. The mapped targets were imported into the DAVID bioinformatics platform, which underwent Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and the network pharmacology prediction results were evaluated through clinical trials.

Results: We obtained 151 compounds related to Huoxue Qingre decoction, 286 genes after evaluation and deduplication, and 426 genes related to coronary heart disease. Finally, 81 common target genes were obtained with 32 pathways according to the KEGG pathway enrichment analysis. The validation results of the clinical trials showed that a total of 98 differential metabolites were found in the treatment of coronary heart disease with Huoxue Qingre decoction, involving a total of 16 metabolic pathways. Compared with the network pharmacology prediction results, it was found that only the pathways in cancer (hsa05200) were the common pathways in the top 32 signaling pathways predicted by network pharmacology. The expanded network pharmacology prediction results revealed that the sphingolipid signaling pathway (hsa04071) and prostate cancer pathway (hsa05215) matched the predicted metabolic pathways, with differential metabolites of N-oleoyl-D-sphingomyelin and 1-methyl-6-phenyl-1h-imidazole[4,5-b]pyridine-2-amine.

Conclusion: Through the network analysis and metabolomic evaluation, there may be three signaling pathways that involve the Huoxue Qingre decoction in the treatment of coronary heart disease: pathways in cancer (hsa05200), sphingolipid signaling pathway (hsa04071), and prostate cancer pathway (hsa05215).