bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolution of Cementite Substructure of Rails from Hypereutectoid Steel during Operation
Authors: Gromov Victor , Ivanov Yurii , Porfiriev Mikhail , Shliarova Yulia
Published: October 2023

Transmission electron microscopy methods were used to analyze the cementite substructure in the head of special-purpose long rails of the DT400IK category, made of hypereutectoid steel, after long-term operation on an experimental track on the Russian Railways ring (the tonnage was 187 million tons). It is noted that the study of various aspects of cementite—its structure, morphology, chemical composition, crystal lattice defects—is relevant. The steel structure is represented by three morphological components at a distance of 10 mm from the sample surface: lamellar perlite, fractured and fragmented perlite. The volume fraction of lamellar perlite in the material is 65%. It is shown that after operation, the cementite plates are bent and separated by ferrite bridges. In the plates of ferrite and cementite, a dislocation substructure is formed, which is of a chaotically distributed and network type in ferrite and of an ordered type in cementite. An increased density of dislocations at the ferrite–cementite interfaces compared to the volume of ferrite plates was noted. Two possible mechanisms of deformation transformation of lamellar perlite grains are indicated: fracture of cementite plates and carbon pulling out from the lattice of the carbide phase. It is indicated that in the dissolution of cementite plates, the interfacial boundaries of “α-phase-cementite” play an important role. The removal of carbon from cementite plates occurs most intensively near defects in ferrite and cementite. The formed nanosized particles of tertiary cementite are unevenly distributed in the ferrite plates; most of them are observed at the locations of ferrite subgrains and interfacial boundaries. This results in non-uniform diffraction contrast in dark-field images of cementite plates. Nanosized particles of cementite can be taken out into the interlamellar space of pearlite colonies in the process of dislocation slip, or they are formed as a result of deformation decomposition, which is less likely. The fragmentation of ferrite and cementite plates is revealed and azimuthal components of total misorientation angles are estimated. The mechanisms of mass transfer of carbon atoms over interstitial sites, deformation vacancies, dislocation tubes, grain boundaries and fragments are considered. According to all the established patterns of the cementite substructure transformation, a comparison with the results for rails made of hypoeutectoid steel was performed.
Heat Treatment Induced Structural Transformations and High-Temperature Oxidation Behavior of Al21Co22Cr22Fe13Ni22 High-Entropy Coatings Produced by Non-Vacuum Electron Beam Cladding
Authors: Ogneva Tatiana , Emurlaev Kemal , Malyutina Yulia , Domarov Evgeny , Chakin Ivan , Ruktuev Alexey , Riabinkina Polina , Yurgin Aleksandr , Bataev Ivan
Published: October 2023

In this study, Al21Co22Cr22Fe13Ni22 high-entropy coatings were produced on steel substrates by non-vacuum electron beam cladding of Co, Cr, and NiAl powders. The high-temperature oxidation behavior of the coatings was studied by holding the specimens at 900 °C in air. The microstructure and phase constitution of the samples were studied both in the as-cladded state and after the heat treatment. The microstructure was characterized using light microscopy (LM) and scanning electron microscopy (SEM). Synchrotron X-ray diffraction (SXRD) and energy-dispersive X-ray spectroscopy (EDX) were used to study the phase constitution of the coatings and the “coating-substrate” interface. The coating consisted of disordered bcc (A2), ordered bcc (B2), and disordered fcc (A1) phases. Annealing the coatings for 50 h at 900 °C led to the formation of fcc precipitates in the bcc dendritic grains and a mixture of fcc and σ-phase particles in the interdendritic regions. Needle-like nanosized B2-precipitates were formed due to annealing in the fcc grains at the coating/substrate interface. The microhardness at the top of the as-cladded coating was 585 HV and gradually decreased towards the substrate. A more uniform distribution of the microhardness was obtained after the annealing. Its average value was 441 HV. Rhomboid Cr2O3, needle-like Al2O3, and spinels of a different morphology were found on the surface of the samples after oxidation at 900 °C.
Enzymatic Cleavage of Stx2a in the Gut and Identification of Pancreatic Elastase and Trypsin as Possible Main Cleavers
Authors: Kellnerová Sára , Huber Silke , Massri Mariam , Fleischer Verena , Losso Klemens , Sarg Bettina , Kremser Leopold , Talasz Heribert , He Xiaohua , Varrone Elisa , Brigotti Maurizio , Ardissino Gianluigi , Orth-Höller Dorothea , Würzner Reinhard
Published: October 2023

Shiga toxins (Stxs), especially the Stx2a subtype, are the major virulence factors involved in enterohemorrhagic Escherichia coli (EHEC)-associated hemolytic uremic syndrome (eHUS), a life-threatening disease causing acute kidney injury, especially in children. After oral transmission and colonization in the gut, EHEC release Stx. Intracellular cleavage of the Stx A subunit, when followed by reduction, boosts the enzymatic activity that causes damage to targeted cells. This cleavage was assumed to be mostly mediated by furin during Stx intracellular trafficking. To investigate whether this cleavage could occur in the intestine, even prior to entering target cells, Stx2a A subunit structure (intact or cleaved) was characterized after its exposure to specific host factors present in human stool. The molecular weight of Stx2a A subunit/fragments was determined by immunoblotting after electrophoretic separation under reducing conditions. In this study, it was demonstrated that Stx2a is cleaved by certain human stool components. Trypsin and chymotrypsin-like elastase 3B (CELA3B), two serine proteases, were identified as potential candidates that can trigger the extracellular cleavage of Stx2a A subunit directly after its secretion by EHEC in the gut. Whether the observed cleavage indeed translates to natural infections and plays a role in eHUS pathogenesis has yet to be determined. If so, it seems likely that a host’s protease profile could affect disease development by changing the toxin’s biological features.
Characterizing the Gut Microbial Communities of Native and Invasive Freshwater Bivalves after Long-Term Sample Preservation
Authors: Vaughn Stephanie N. , Atkinson Carla L. , Johnson Paul D. , Jackson Colin R.
Published: October 2023

Freshwater mussels are important indicators of the overall health of their environment but have suffered declines that have been attributed to factors such as habitat degradation, a loss of fish hosts, climate change, and excessive nutrient inputs. The loss of mussel biodiversity can negatively impact freshwater ecosystems such that understanding the mussel’s gut microbiome has been identified as a priority topic for developing conservation strategies. In this study, we determine whether ethanol-stored specimens of freshwater mussels can yield representative information about their gut microbiomes such that changes in the microbiome through time could potentially be determined from museum mussel collections. A short-term preservation experiment using the invasive clam Corbicula fluminea was used to validate the use of ethanol as a method for storing the bivalve microbiome, and the gut microbiomes of nine native mussel species that had been preserved in ethanol for between 2 and 9 years were assessed. We show that ethanol preservation is a valid storage method for bivalve specimens in terms of maintaining an effective sequencing depth and the richness of their gut bacterial assemblages and provide further insight into the gut microbiomes of the invasive clam C. fluminea and nine species of native mussels. From this, we identify a “core” genus of bacteria (Romboutsia) that is potentially common to all freshwater bivalve species studied. These findings support the potential use of ethanol-preserved museum specimens to examine patterns in the gut microbiomes of freshwater mussels over long periods.
Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity
Authors: Hernández Flores José Luis , Martínez Yomaiko Javier , Ramos López Miguel Ángel , Saldaña Gutierrez Carlos , Reyes Aldo Amaro , Armendariz Rosales Mariem Monserrat , Cortés Pérez Maraly Jazmin , Mendoza Mayela Fosado , Ramírez Ramírez Joanna , Zavala Grecia Ramírez , Tovar Becerra Paola Lizeth , Valdez Santoyo Laila , Villasana Rodríguez Karen , Rodríguez Morales José Alberto , Campos Guillén Juan
Published: October 2023

The Kosakonia cowanii Cp1 strain was isolated from seeds of Capsicum pubescens R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity test revealed nonpathogenic traits confirmed by the absence of specific virulence-related genes. Interestingly, when fungal inhibitory essays were carried out, the bacterial synthesis of volatile organic compounds (VOCs) with antifungal activity showed that Sclerotinia sp. and Rhizoctonia solani were inhibited by 87.45% and 77.24%, respectively. Meanwhile, Sclerotium rolfsii, Alternaria alternata, and Colletotrichum gloeosporioides demonstrated a mean radial growth inhibition of 52.79%, 40.82%, and 55.40%, respectively. The lowest inhibition was by Fusarium oxysporum, with 10.64%. The VOCs’ characterization by headspace solid–phase microextraction combined with gas chromatography–mass spectrometry (HS-SPME-GC–MS) revealed 65 potential compounds. Some of the compounds identified with high relative abundance were ketones (22.47%), represented by 2-butanone, 3-hydroxy (13.52%), and alcohols (23.5%), represented by ethanol (5.56%) and 1-butanol-3-methyl (4.83%). Our findings revealed, for the first time, that K. cowanii Cp1 associated with C. pubescens seeds possesses potential traits indicating that it could serve as an effective biocontrol.
Theoretical Research on the Reduction of CO2 with H2S on Pyrite FeS2 Surfaces
Authors: Liu Yingchao , Li Yuqiong , Feng Yao , Chen Jianhua , Zhao Cuihua
Published: October 2023

Understanding the reduction of CO2 and the origin and evolution of early life on Earth is an important research endeavor. Pyrite, due to its semiconductor properties, is believed to play a pivotal role as a reactant or catalyst in converting reducing gases, such as CO2, into organic matter. In this study, we employed density functional theory (DFT) to investigate the reduction of CO2 in the presence of H2S on the surface of pyrite. Our findings reveal that the presence of sulfur vacancies enhances the adsorption of H2S and CO2 molecules onto the pyrite surface. Interestingly, we observed the generation of the HCOOH molecule on the defective pyrite surface. Additionally, the transition state analysis indicates that H2S and CO2 molecules require the overcoming of an energy barrier (Ea) of 36.93 kJ/mol to form the HCOOH molecule. This study sheds light on the role of pyrite in the early creation of life on Earth by elucidating its impact on the reduction of carbon dioxide.
Discovery of 7-Azanorbornane-Based Dual Agonists for the Delta and Kappa Opioid Receptors through an In Situ Screening Protocol
Authors: Karaki Fumika , Takamori Taro , Kawakami Koumei , Sakurai Sae , Hidaka Kyoko , Ishii Kei , Oki Tomoya , Sato Noriko , Atsumi Nao , Ashizawa Karin , Taguchi Ai , Ura Asuka , Naruse Toko , Hirayama Shigeto , Nonaka Miki , Miyano Kanako , Uezono Yasuhito , Fujii Hideaki
Published: October 2023

In medicinal chemistry, the copper-catalyzed click reaction is used to prepare ligand candidates. This reaction is so clean that the bioactivities of the products can be determined without purification. Despite the advantages of this in situ screening protocol, the applicability of this method for transmembrane proteins has not been validated due to the incompatibility with copper catalysts. To address this point, we performed ligand screening for the µ, δ, and κ opioid receptors using this protocol. As we had previously reported the 7-azanorbornane skeleton as a privileged scaffold for the G protein-coupled receptors, we performed the click reactions between various 7-substituted 2-ethynyl-7-azanorbornanes and azides. Screening assays were performed without purification using the CellKeyTM system, and the putative hit compounds were re-synthesized and re-evaluated. Although the “hit” compounds for the µ and the δ receptors were totally inactive after purifications, three of the four “hits” for the κ receptor were true agonists for this receptor and also showed activities for the δ receptor. Although false positive/negative results exist as in other screening projects for soluble proteins, this in situ method is effective in identifying novel ligands for transmembrane proteins.
Diversity, Antimicrobial, Antioxidant, and Anticancer Activity of Culturable Fungal Endophyte Communities in Cordia dichotoma
Authors: Sharma Mahima , Bharti Sahil , Goswami Anindya , Mallubhotla Sharada
Published: October 2023

Endophytic fungi are a significant source of secondary metabolites, which are chemical compounds with biological activities. The present study emphasizes the first-time isolation and identification of such fungi and their pharmacological activities from the medicinal plant Cordia dichotoma, which is native to Jammu, India. The Shannon Wiener diversity index revealed a wide range of fungal endophytes in root (1.992), stem (1.645), and leaf (1.46) tissues. A total of 19 endophytic fungi belonging to nine different genera were isolated from this plant and the majority belonged to the Ascomycota phylum. ITS rRNA gene sequencing was used to identify the fungal strains and they were submitted in NCBI GenBank. The most potent fungal isolate Cladosporium cladosporioides OP870014 had strong antimicrobial, antioxidant, and anticancer activity against MCF-7, HCT-116, and PC-3 cancer cell lines. The LC-MS and GC-MS analyses of the ethyl acetate extract of C. cladosporioides were examined to identify the bioactive metabolites. The major compounds of the crude extract derived from C. cladosporioides OP870014, according to GC-MS, are spiculisporic acid; dibutyl phthalate; phenylethyl alcohol; cyclohexanone, 2,3,3-trimethyl-2-3-methylbutyl; pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(phenylmethyl);2,5-piperazinedione,3,6-bis(2-methylpropyl); and heneicosane which possessed antimicrobial, anticancerous, and antioxidant activities. The findings revealed that C. dichotoma has the capacity to host a wide variety of fungal endophytes and that secondary metabolites from the endophytic fungus may be a source of alternative naturally occurring antimicrobial, antioxidant, and cytotoxic compounds.
Combination NIPS/TIPS Synthesis of α-Fe2O3 and α/γ-Fe2O3 Doped PVDF Composite for Efficient Piezocatalytic Degradation of Rhodamine B
Authors: Magomedova Asiyat G. , Rabadanova Alina A. , Shuaibov Abdulatip O. , Selimov Daud A. , Sobola Dinara S. , Rabadanov Kamil Sh. , Giraev Kamal M. , Orudzhev Farid F.
Published: October 2023

Highly porous membranes based on polyvinylidene fluoride (PVDF) with the addition of nanoscale particles of non-magnetic and magnetic iron oxides were synthesized using a combined method of non-solvent induced phase separation (NIPS) and thermo-induced phase separation (TIPS) based on the technique developed by Dr. Blade. The obtained membranes were characterized using SEM, EDS, XRD, IR, diffuse reflectance spectroscopy, and fluorescent microscopy. It was shown that the membranes possessed a high fraction of electroactive phase, which increased up to a maximum of 96% with the addition of 2 wt% of α-Fe2O3 and α/γ-Fe2O3 nanoparticles. It was demonstrated that doping PVDF with nanoparticles contributed to the reduction of pore size in the membrane. All membranes exhibited piezocatalytic activity in the degradation of Rhodamine B. The degree of degradation increased from 69% when using pure PVDF membrane to 90% when using the composite membrane. The nature of the additive did not affect the piezocatalytic activity. It was determined that the main reactive species responsible for the degradation of Rhodamine B were •OH and •O2−. It was also shown that under piezocatalytic conditions, composite membranes generated a piezopotential of approximately 2.5 V.
Molecular Simulation of Coal Molecular Diffusion Properties in Chicheng Coal Mine
Authors: Yan Jingxue , Jia Baoshan , Liu Baogang , Zhang Jinyi
Published: October 2023

In order to study the importance of the diffusion mechanism of CH4 and CO2 in coal for the development of coalbed methane, the aim of this paper is to reveal the influence mechanism of pressure, temperature, water content and other factors on the molecular diffusion behavior of gas at the molecular level. In this paper, non-sticky coal in Chicheng Coal Mine is taken as the research object. Based on the molecular dynamics method (MD) and Monte Carlo (GCMC) method, the diffusion characteristics and microscopic mechanism of CH4 and CO2 in coal under different pressures (100 kPa–10 MPa), temperatures (293.15–313.15 K) and water contents (1–5%) were analyzed in order to lay a theoretical foundation for revealing the diffusion characteristics of CBM in coal, and provide technical support for further improving CBM extraction. The results show that high temperature is conducive to gas diffusion, while high pressure and water are not conducive to gas diffusion in the coal macromolecular model.
Chemical Composition and Anti-Candida Activity of Mentha suaveolens Ehrh. Essential Oils Obtained by Different Distillation Processes
Authors: Tadić Vanja , Božović Mijat , Sapienza Filippo , Astolfi Roberta , Mladenović Milan , Zaka Maria Cristina , Del Bove Fabiana , Borzacchi Francesca , Fraschetti Caterina , Rossi Caterina , Vertuani Silvia , Baldisserotto Anna , Manfredini Stefano , Ragno Rino
Published: October 2023

A comparative study on essential oils extracted from Mentha suaveolens Ehrh. from Italy is reported. Two extraction procedures were investigated: hydrodistillation and steam distillation, carried out as a continuous and fractionated procedure. Fresh and dried plant material from two harvests was used. The hydrodistillation method yielded a higher amount of essential oil. The dried plant was significantly richer in essential oil per kg of starting plant material. Gas chromatography-mass spectrometry analysis of 112 samples showed that the essential oils belong to the piperitenone oxide-rich chemotype. In addition, piperitenone, p-cymen-8-ol, and limonene were among the most abundant compounds in the different samples. A higher amount of piperitenone oxide was obtained by hydrodistillation, while steam distillation gave a higher percentage of piperitenone and limonene. The essential oils were characterized for their anti-Candida albicans activity; higher potency was observed for the samples rich in piperitenone oxide, with MIC values ranging from 0.39 to 0.78 mg·mL−1 (0.039% and 0.078% p/v). The results of this work provide a deep insight into the methodology of essential oil extraction and the associated chemical variability of M. suaveolens Ehrh. Some of the essential oils are potent against C. albicans and could be considered for potential use in therapy.
Visualization of Hot Carrier Dynamics in a Single CsPbBr3 Perovskite Microplate Using Femtosecond Kerr-Gated Wide-Field Fluorescence Spectroscopy
Authors: Huang Zhenqiang , Tan Wenjiang , Ma Peipei , Yan Lihe , Si Jinhai , Hou Xun
Published: October 2023

Lead halide perovskites (LHPs) have excellent semiconductor properties. They have been used in many applications such as solar cells. Recently, the hot carrier dynamics in this type of material have received much attention as they are useful for enhancing the performance of optoelectrical devices fabricated from it. Here, we study the ultrafast hot carrier dynamics of a single CsPbBr3 microplate using femtosecond Kerr-gated wide-field fluorescence spectroscopy. The transient photoluminescence spectra have been measured under a variety of excitation fluences. The temporal evolution of bandgap renormalization and the competition between hot carrier cooling and the recovery of the renormalized bandgap are clearly revealed.
Mechanical and Optical Properties of Cr2O3 Thin Films Grown by Atomic Layer Deposition Method Using Cr(thd)3 and Ozone
Authors: Salari Mehr Mahtab , Aarik Lauri , Jõgiaas Taivo , Kasikov Aarne , Damerchi Elyad , Mändar Hugo
Published: October 2023

Cr2O3 thin films were grown on a Si (1 0 0) substrate using Cr(thd)3 and O3 by atomic layer deposition (ALD) at substrate temperatures (TG) from 200 to 300 °C. X-ray amorphous films were deposited at a TG ≤ 225 °C, whereas at higher temperatures (TG ≥ 250 °C), the eskolaite phase was observed in the films. The growth rate of the films increased from 0.003 to 0.01 nm/cycle by increasing TG from 200 to 275 °C. The relatively low growth rate of Cr(thd)3—O3 makes it appropriate for the ALD of precisely controllable solid solution-type ternary-component thin films. The Ti-doped Cr2O3 film showed higher hardness (16.7 GPa) compared with that of the undoped film (12.8 GPa) with similar thickness. The band gap values of the pure Cr2O3 corresponding to the indirect transition model showed no dependence on TG; however, doping the Cr2O3 with Ti decreased its band gap energy value from 3.1 to 2.2 eV.
Synergistic Enhancement of Near-Infrared Emission in CsPbCl3 Host via Co-Doping with Yb3+ and Nd3+ for Perovskite Light Emitting Diodes
Authors: Padhiar Muhammad Amin , Zhang Shaolin , Wang Minqiang , Zamin Khan Noor , Iqbal Shoaib , Ji Yongqiang , Muhammad Nisar , Khan Sayed Ali , Pan Shusheng
Published: October 2023

Perovskite nanocrystals (PeNCs) have emerged as a promising class of luminescent materials offering size and composition-tunable luminescence with high efficiency and color purity in the visible range. PeNCs doped with Yb3+ ions, known for their near-infrared (NIR) emission properties, have gained significant attention due to their potential applications. However, these materials still face challenges with weak NIR electroluminescence (EL) emission and low external quantum efficiency (EQE), primarily due to undesired resonance energy transfer (RET) occurring between the host and Yb3+ ions, which adversely affects their emission efficiency and device performance. Herein, we report the synergistic enhancement of NIR emission in a CsPbCl3 host through co-doping with Yb3+/Nd3+ ions for perovskite LEDs (PeLEDs). The co-doping of Yb3+/Nd3+ ions in a CsPbCl3 host resulted in enhanced NIR emission above 1000 nm, which is highly desirable for NIR optoelectronic applications. This cooperative energy transfer between Yb3+ and Nd3+ can enhance the overall efficiency of energy conversion. Furthermore, the PeLEDs incorporating the co-doped CsPbCl3/Yb3+/Nd3+ PeNCs as an emitting layer exhibited significantly enhanced NIR EL compared to the single doped PeLEDs. The optimized co-doped PeLEDs showed improved device performance, including increased EQE of 6.2% at 1035 nm wavelength and low turn-on voltage. Our findings highlight the potential of co-doping with Yb3+ and Nd3+ ions as a strategy for achieving synergistic enhancement of NIR emission in CsPbCl3 perovskite materials, which could pave the way for the development of highly efficient perovskite LEDs for NIR optoelectronic applications.
Lactobacillus reuteri Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Modulating the Gut Microbiota in Mice
Authors: Shen Jian , Wang Shuting , Huang Yong , Wu Zhengjie , Han Shengyi , Xia He , Chen Hui , Li Lanjuan
Published: October 2023

Acute lung injury (ALI) causes lung inflammation and edema as well as resulting in gut microbiota disorder. Probiotics, however, can improve the gut microbiota composition and modulate its immune response, playing an important role in ALI pathogenesis. Therefore, our study aims to investigate the effect of Lactobacillus reuteri on Lipopolysaccharide (LPS)-induced ALI in mice and to probe the mechanism of its synergistic modulatory effect on the lungs and intestines. We assessed the therapeutic effects of L. reuteri in the ALI mouse model by histopathology, alveolar lavage fluid and serum inflammatory factor analysis and explored microbiome and transcriptome alterations. L. reuteri intervention effectively attenuated lung tissue injury and significantly reduced the LPS-induced inflammatory response and macrophage and neutrophil infiltration. Additionally, L. reuteri improved the intestinal barrier function and remodeled the disordered microbiota. In conclusion, our study showed that L. reuteri attenuated the inflammatory response, ameliorated the pulmonary edema, repaired the intestinal barrier, and remodeled the gut microbiota in ALI mice. This study provides new perspectives on the clinical treatment of ALI.
The Large American Liver Fluke (Fascioloides magna): A Survivor’s Journey through a Constantly Changing World
Authors: Csivincsik Ágnes , Halász Tibor , Nagy Gábor
Published: October 2023

The large American liver fluke (Fascioloides magna) is considered an invasive trematode parasite in Europe. Its origin dates back before the Cretaceous-Paleogene Mass Extinction, after which it survived at least three population bottlenecks and successful host switches before it arrived in Europe. The authors review the evolutionary history of F. magna, the distribution by its ancient proboscidean hosts, and the probable drivers of the switch to the white-tailed deer (Odocoileus virginianus). The review collects knowledge on the biology of intermediate hosts, which helps understand the factors that influence the epidemiology of F. magna in aquatic ecosystems. The authors demonstrate the adaptation potential of the parasite using data from both North American and European endemics. Finally, the study calls attention to the epidemiological risk of human-induced global change, with a special interest in the invasive snail species Pseudosuccinea columella.
The Virucidal Effect of the Chlorination of Water at the Initial Phase of Disinfection May Be Underestimated If Contact Time Calculations Are Used
Authors: Saguti Fredy , Kjellberg Inger , Churqui Marianela Patzi , Wang Hao , Tunovic Timur , Ottoson Jakob , Bergstedt Olof , Norder Helene , Nyström Kristina
Published: October 2023

For the microbiological safety of drinking water, disinfection methods are used to remove or inactivate microorganisms. Chlorine and chlorine dioxide are often used as disinfectants in drinking water treatment plants (DWTPs). We investigated the effectiveness of these chemicals in inactivate echovirus 30 (E30), simian 11 rotavirus (RV SA11), and human adenovirus type 2 (HAdV2) in purified water from a DWTP. Within two minutes of contact, chlorine dioxide inactivated E30 by 4-log10, RV SA11 by 3-log10, and HAdV2 could not be detected, while chlorine reduced E30 by 3-log10, RV SA11 by 2–3log10, and HAdV2 by 3–4log10. However, viral genomes could be detected for up to 2 h using qPCR. The CT method, based on a combination of disinfectant concentration and contact time, during such a short initial phase, is problematic. The high concentrations of disinfectant needed to neutralize organic matter may have a strong immediate effect on virus viability. This may lead to the underestimation of disinfection and overdosing of disinfectants in water with organic contamination. These results are useful for the selection of disinfection systems for reuse of treated wastewater and in the risk assessment of water treatment processes using chlorine and chlorine dioxide.
Subconjunctival Delivery of Sorafenib-Tosylate-Loaded Cubosomes for Facilitated Diabetic Retinopathy Treatment: Formulation Development, Evaluation, Pharmacokinetic and Pharmacodynamic (PKPD) Studies
Authors: Madhusudhan Sharadha , Gupta Naresh Vishal , Rahamathulla Mohamed , Chidambaram Saravana Babu , Osmani Riyaz Ali M. , Ghazwani Mohammed , Ahmed Mohammed Muqtader , Farhana Syeda Ayesha , Sarhan Mohammed Y. , Tousif Ahmed Hediyal
Published: October 2023

Diabetic retinopathy (DR) is a microvascular complication associated with vascular endothelial growth factor (VEGF) overexpression. Therapeutic delivery to the retina is a challenging phenomenon due to ocular biological barriers. Sorafenib tosylate (ST) is a lipophilic drug with low molecular weight, making it ineffective at bypassing the blood–retinal barrier (BRB) to reach the target site. Cubosomes are potential nanocarriers for encapsulating and releasing such drugs in a sustained manner. The present research aimed to compare the effects of sorafenib-tosylate-loaded cubosome nanocarriers (ST-CUBs) and a sorafenib tosylate suspension (ST-Suspension) via subconjunctival route in an experimental DR model. In this research, ST-CUBs were prepared using the melt dispersion emulsification technique. The distribution of prepared nanoparticles into the posterior eye segments was studied with confocal microscopy. The ST-CUBs were introduced into rats’ left eye via subconjunctival injection (SCJ) and compared with ST-Suspension to estimate the single-dose pharmacokinetic profile. Streptozotocin (STZ)-induced diabetic albino rats were treated with ST-CUBs and ST-Suspension through the SCJ route once a week for 28 days to measure the inhibitory effect of ST on the diabetic retina using histopathology and immunohistochemistry (IHC) examinations. Confocal microscopy and pharmacokinetic studies showed an improved concentration of ST from ST-CUBs in the retina. In the DR model, ST-CUB treatment using the SCJ route exhibited decreased expression levels of VEGF, pro-inflammatory cytokines, and adhesion molecules compared to ST-Suspension. From the noted research findings, it was concluded that the CUBs potentially enhanced the ST bioavailability. The study outcomes established that the developed nanocarriers were ideal for delivering the ST-CUBs via the SCJ route to target the retina for facilitated DR management.
On the Performance of Intelligent Reconfigurable Surfaces for 6G Indoor Visible Light Communications Systems
Authors: Cañizares Milton Román , Játiva Pablo Palacios , Guaña-Moya Javier , Villegas-Ch William , Azurdia-Meza Cesar
Published: October 2023

Indoor visible light communication (VLC) systems have been extensively studied; however, they present some problems when the main propagation channel component, called Line-of-Sight (LoS), is partially or totally blocked. The effect of this blockage can cause degradation of the received optical signal and decrease the performance of the VLC system. Therefore, in recent years, research has focused on proposing solutions to mitigate this issue. Under this context, in this paper, we propose the use and implementation of intelligent reconfigurable surfaces (IRS) in the VLC indoor system to improve the propagation channel component produced by reflections, called non-line-of-sight (Non-LoS). Furthermore, we have analyzed and established the mathematical expressions of the channel components, including the effect of the IRS on the VLC system. These expressions have been evaluated in a simulated indoor VLC scenario in terms of the channel impulse response (CIR) and the bit error rate (BER). The findings achieved allow us to demonstrate that the use of IRS in the VLC system improves the performance of the system in terms of the parameters evaluated. These results also allow us to highlight the possible use of improvements in the VLC system for inclusion as a 6G enabling technology.
Polarization Lidar: Principles and Applications
Authors: Liu Xudong , Zhang Liping , Zhai Xiaoyu , Li Liye , Zhou Qingji , Chen Xue , Li Xiaobo
Published: October 2023

Traditional lidar techniques mainly rely on the backscattering/echo light intensity and spectrum as information sources. In contrast, polarization lidar (P-lidar) expands the dimensions of detection by utilizing the physical property of polarization. By incorporating parameters such as polarization degree, polarization angle, and ellipticity, P-lidar enhances the richness of physical information obtained from target objects, providing advantages for subsequent information analysis. Over the past five decades, the application fields of P-lidar have rapidly expanded, starting from its early use in atmospheric remote sensing to later applications in oceanic remote sensing. This review first provides a brief introduction to the basic principles of both polarization and P-lidar, along with typical systems. It then explores the applications of P-lidar in various remote sensing fields, including atmospheric, oceanic, and terrestrial domains. Additionally, we propose potential research directions based on current cutting-edge applications, with the aims of providing critical insights to researchers in the fields of polarization and lidar and inspiring further exciting ideas.
Gravitational Ischemia in the Brain: How Interfering with Its Release May Predispose to Either Alzheimer’s- or Parkinson’s-like Illness, Treatable with Hyperbaric Oxygen
Authors: Jaster J. Howard , Ottaviani Giulia
Published: October 2023

The physiological mechanisms for releasing and resolving gravitational ischemia in the brain, and their susceptibility to malfunction, may play an important role in a variety of neurological illnesses. An astronaut on a space walk in a micro-gravity environment may be susceptible to neuro-ocular symptoms associated with unopposed gravity-resistance mechanisms for partially preventing gravitational ischemia in the brain, and for attenuating its impact—mechanisms which may be required for normal brain physiology on Earth. Astronauts on the International Space Station typically breathe a mixture of gasses similar in composition to what they breathed on Earth, following the 1967 death of three astronauts, including Ed White, by fire on the Apollo 1 spacecraft, which was carrying 100% oxygen. For the last decade, astronauts have been studied extensively by flight physicians regarding the commonly experienced symptoms of VIIP, or ‘visual impairment and intracranial pressure’ syndrome. In this paper, we compare VIIP syndrome to the neuro-ocular and Parkinson’s-like symptoms which occurred during and after the 1918–1919 influenza pandemic. The common denominator may be gravitational ischemia in the brain, and the mirror-imaging failed mechanisms for its release (in influenza) versus unopposed gravity-resistance mechanisms (in astronauts). Some research has suggested that astronauts may benefit from breathing oxygen concentrations somewhat higher than 20%, and under slightly elevated pressure. These may possibly prevent maladaptive mechanisms leading to Alzheimer’s- or Parkinson’s-like illness by compensating for impaired mechanisms for releasing and resolving gravitational ischemia in the brain.
Advances in Roles of Salicylic Acid in Plant Tolerance Responses to Biotic and Abiotic Stresses
Authors: Song Weiyi , Shao Hongbo , Zheng Aizhen , Zhao Longfei , Xu Yajun
Published: October 2023

A multitude of biotic and abiotic stress factors do harm to plants by bringing about diseases and inhibiting normal growth and development. As a pivotal signaling molecule, salicylic acid (SA) plays crucial roles in plant tolerance responses to both biotic and abiotic stresses, thereby maintaining plant normal growth and improving yields under stress. In view of this, this paper mainly discusses the role of SA in both biotic and abiotic stresses of plants. SA regulates the expression of genes involved in defense signaling pathways, thus enhancing plant immunity. In addition, SA mitigates the negative effects of abiotic stresses, and acts as a signaling molecule to induce the expression of stress-responsive genes and the synthesis of stress-related proteins. In addition, SA also improves certain yield-related photosynthetic indexes, thereby enhancing crop yield under stress. On the other hand, SA acts with other signaling molecules, such as jasmonic acid (JA), auxin, ethylene (ETH), and so on, in regulating plant growth and improving tolerance under stress. This paper reviews recent advances in SA’s roles in plant stress tolerance, so as to provide theoretical references for further studies concerning the decryption of molecular mechanisms for SA’s roles and the improvement of crop management under stress.
Sustainable Strategies for Synthesizing Lignin-Incorporated Bio-Based Waterborne Polyurethane with Tunable Characteristics
Authors: Kim Bo Min , Choi Jin Sil , Jang Sunjin , Park Hyeji , Lee Seung Yeol , Jung Joonhoo , Park Jaehyeung
Published: October 2023

In this study, we introduce a novel approach for synthesizing lignin-incorporated castor-oil-based cationic waterborne polyurethane (CWPU-LX), diverging significantly from conventional waterborne polyurethane dispersion synthesis methods. Our innovative method efficiently reduces the required solvent quantity for CWPU-LX synthesis to approximately 50% of that employed in traditional WBPU experimental procedures. By incorporating lignin into the polyurethane matrix using this efficient and reduced-solvent method, CWPU-LX demonstrates enhanced properties, rendering it a promising material for diverse applications. Dynamic interactions between lignin and polyurethane molecules contribute to improved mechanical properties, enhanced thermal stability, and increased solvent resistance. Dynamic interactions between lignin and polyurethane molecules contribute to improved tensile strength, up to 250% compared to CWPU samples. Furthermore, the inclusion of lignin enhanced thermal stability, showcasing a 4.6% increase in thermal decomposition temperature compared to conventional samples and increased solvent resistance to ethanol. Moreover, CWPU-LX exhibits desirable characteristics such as protection against ultraviolet light and antibacterial properties. These unique properties can be attributed to the presence of the polyphenolic group and the three-dimensional structure of lignin, further highlighting the versatility and potential of this material in various application domains. The integration of lignin, a renewable and abundant resource, into CWPU-LX exemplifies the commitment to environmentally conscious practices and underscores the significance of greener materials in achieving a more sustainable future.
Continuous-Flow System for Methylene Blue Removal Using a Green and Cost-Effective Starch Single-Rod Column
Authors: Taweekarn Tarawee , Wongniramaikul Worawit , Sriprom Wilasinee , Limsakul Wadcharawadee , Choodum Aree
Published: October 2023

A continuous-flow system based on a green and cost-effective monolithic starch cryogel column was successfully developed for removing methylene blue (MB). The proposed column exhibited high removal efficiency (up to 99.9%) and adsorption capacity (25.4 mg·g−1) for synthetic and real samples with an adsorbent cost of USD 0.02. The influence of various operation parameters, including the flow rate, initial concentration, column height, and temperature, on the MB removal efficiency was examined and reported. The MB removal efficiency remained >99% in the presence of potential interferences, highlighting the good performance of the cryogel column. The Yoon–Nelson dynamic model explained the MB adsorption better than the Bohart–Adams model, as indicated by the higher R2 values (R2 = 0.9890–0.9999) exhibited by the former and current trends of its parameters. The MB removal efficiency of the cryogel column remained at 62.7% after three reuse cycles. The wastewater containing MB collected from a local batik-production community enterprise in Phuket, Thailand was applied to the proposed continuous-flow system under optimum conditions, and results indicated that 99.7% of the MB present in 2.4 L of wastewater was removed. These results validate the excellent application potential of the cryogel column for the continuous-flow adsorption of MB. This study will facilitate future industrial applications and process designs of the continuous-flow system.
Physicochemical Pretreatment of Vietnamosasa pusilla for Bioethanol and Xylitol Production
Authors: Wongleang Suwanan , Premjet Duangporn , Premjet Siripong
Published: October 2023

The consumption of fossil fuels has resulted in severe environmental consequences, including greenhouse gas emissions and climate change. Therefore, transitioning to alternative energy sources, such as cellulosic ethanol, is a promising strategy for reducing environmental impacts and promoting sustainable low-carbon energy. Vietnamosasa pusilla, an invasive weed, has been recognized as a high potential feedstock for sugar-based biorefineries due to its high total carbohydrate content, including glucan (48.1 ± 0.3%) and xylan (19.2 ± 0.4%). This study aimed to examine the impact of NaOH pretreatment-assisted autoclaving on V. pusilla feedstock. The V. pusilla enzymatic hydrolysate was used as a substrate for bioethanol and xylitol synthesis. After treating the feedstock with varying concentrations of NaOH at different temperatures, the glucose and xylose recovery yields were substantially higher than those of the untreated material. The hydrolysate generated by enzymatic hydrolysis was fermented into bioethanol using Saccharomyces cerevisiae TISTR 5339. The liquid byproduct of ethanol production was utilized by Candida tropicalis TISTR 5171 to generate xylitol. The results of this study indicate that the six- and five-carbon sugars of V. pusilla biomass have great potential for the production of two value-added products (bioethanol and xylitol).
Adsorption of Low Density Lipoproteins on an Azobenzene-Containing Polymer Brush: Modelling by Coarse-Grained Molecular Dynamics
Authors: Ilnytskyi Jaroslav , Yaremchuk Dmytro , Komarytsia Orest
Published: October 2023

One of the strategies to reduce the contents of low density lipoproteins (LDLs) in blood is a hemoperfusion, when they are selectively retracted from plasma by an adsorber located outside the patient’s body. Recently, a photo-controllable smart surface was developed experimentally, that is characterized by high selectivity and reusability. It comprises a nanocarrier functionalised by a brush of azobenzene-containing polymer chains. We present a mesoscopic model that mimics principal features of this setup and focus our study on the role played by the length and flexibility of the chains and grafting density of a brush. The model for LDL comprises a spherical core covered by a shell of model phospholipids. The model is studied via coarse-grained molecular dynamics simulation, performed with the aid of the GMBOLDD package modified for the case of soft-core beads. We examined the dependencies of the binding energy on both the length of polymer chains and the grafting density of a brush and established optimal conditions for adsorption. These are explained by competition between the concentration of azobenzenes and phospholipids in the same spatial region, flexibility of polymer chains, and excluded volume effects.
Exploring Islamic Spiritual Care: What Is in a Name?
Authors: Baig Naveed , Isgandarova Nazila
Published: October 2023

At present, there is limited theoretical clarity on the nature of Islamic spiritual care, which is a developing discipline around the world. How it is defined will be instrumental for spiritual care institutions, professionals and recipients of spiritual care in the years to come. This article wishes to understand and explore the idea and vision behind Islamic spiritual care and why this line of investigation may be of importance to care providers with different faith backgrounds.
Hashtagged and Black? A South African Black Theological Engagement from Stellenbosch with Contemporary Student Movements
Authors: Nel Reginald Wilfred
Published: October 2023

Hashtag movements, also amongst contemporary student movements, present a new charge towards decolonization. This also happens at the Faculty of Theology of Stellenbosch University. The group also identified as a Black collective. This contribution argues that this charge is therefore at a deeper level, directed at older generations of Black theologians, and this is assessed critically through a reading of some proponents of third-generation South African Black theologians. It is concluded that there needs to be a conscious nurture of creative tension and challenge, transformative encounters to decolonize theological education in Africa.
Visual and Artistic Expressions in the Service of Catechesis
Authors: Barić Denis
Published: October 2023

Over the course of history, humans have had a deep need to express themselves not only through words but also through images. Furthermore, images and artworks have the potential to serve as a path to God. This article aims to explore the presence of images and artistic expressions in catechesis and their role in education and growth in faith throughout a person’s life. In this regard, the article is structured into three sections, also including an introduction and a conclusion. The first section elaborates on the power of imagery in today’s world, emphasising the human need to express oneself visually as well as verbally and highlighting the significance of all factors that contribute to the interpretation and understanding of images. The second section discusses catechesis, which serves the purpose of an individual’s education and growth in faith, emphasising various methods and ways of teaching in faith. This section also underscores the place and role of images and works of art in the history of the Church and in catechisms. Finally, the third section highlights the importance of aesthetic and artistic formation among all participants in catechesis, whereby the images and artworks are used to facilitate a better understanding and experience of the faith and its content in all its beauty.
The Extent to Which Hemp Insulation Materials Can Be Used in Canadian Residential Buildings
Authors: Liu Chun Hua Julia , Pomponi Francesco , D’Amico Bernardino
Published: October 2023

The embodied carbon of building materials is a significant contributor to greenhouse gas (GHG) emissions. Hemp is widely acknowledged as the most used vegetal insulation in building and construction due to its comparable thermal properties and better environmental performance than that of mainstream insulation materials (MIMs). However, the application of hemp insulation materials (HIMs) in Canada is still in its infancy. Canada is currently the largest hemp oil and seed producer in the world. Most recent research on hemp in Canada has focused on the impact of legalising marijuana and the popularisation of hemp health products and cannabidiol (CBD). There is a lack of studies addressing the holistic impact of hemp in reducing emissions in Canadian residential buildings. This paper exams the feasibility of large-scale hemp cultivation in Canada and the suitability of HIMs for Canadian private dwellings. Material flow analysis (MFA) and life cycle assessment (LCA) were applied to evaluate different levels of carbon mitigation over time produced by HIM substitution. The results show that Canada has sufficient farmland and perfect geographic location and weather to implement large-scale hemp cultivation. HIM substitution can be accomplished for 81% of Canadian residential buildings. Full HIM substitution fulfilled through 5% hemp fibre insulation (HF) and 95% hempcrete (HC) will mitigate 101% of the GHG emissions caused by existing MIMs and contribute up to a 7.38% reduction in emissions to achieve the net zero emissions target by 2050.