bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Austemperability of Thin-wall Ductile Cast Iron Produced by High-Pressure Die-casting
Authors: van gen Hassend Frederic , Ninnemann Lukas , Töberich Felix , Breuckmann Michael , Röttger Arne , Weber Sebastian
Published: September 2021

The production of thin-wall ductile iron (TWDI) by high-pressure die-casting (HPDC) is complex because of several metallurgical and microstructural challenges. The present work aims to evaluate the austemperability of components (4 mm thickness) produced by HPDC process. The graphitization kinetics, the pearlite formation during continuous cooling, and the effect of austempering on the evolution of the ausferritic microstructure were investigated using dilatometric tests, microstructural analysis as well as Vickers hardness tests and tensile tests. Results show that components exhibit a brittle behavior because of white structures, small shrinkage cavities, and microporosity in the as-cast condition. Graphitization at 1100 °C allows rapid formation of small graphite particles within a short time (40 s). The critical cooling time (t8/5) to avoid the formation of pearlite upon cooling was found to be 5 s at a martensite start temperature of 193 ± 14 °C. Austempering at 360 °C for 40 min results in an ausferritic microstructure with stable carbon-enriched austenite which provides a high hardness (355 ± 4 HV10) and tensile strength (Rm = 709 ± 65 MPa). The results represent main criteria regarding the producibility of die-casted TWDI, which are helpful for future alloy and heat treatment design.
Genetic differentiation in functional traits among wild cherry (Prunus avium L.) half-sib lines
Authors: Stojnić Srđan , Kovačević Branislav , Kebert Marko , Vasić Verica , Vuksanović Vanja , Trudić Branislav , Orlović Saša
Published: September 2021

Understanding intra-specific variation in leaf functional traits is one of the key requirements for the evaluation of species adaptive capacity to ongoing climate change, as well as for designing long-term breeding and conservation strategies. Hence, data of 19 functional traits describing plant physiology, antioxidant properties, anatomy and morphology were determined on 1-year-old seedlings of wild cherry (Prunus aviumL.) half-sib lines. The variability within and among half-sib lines, as well as the estimation of multi-trait association, were examined using analysis of variance (ANOVA) followed by Tukey's honestly significant difference test and multivariate analyses: principal component analysis (PCA), canonical discriminant analysis (CDA) and stepwise discriminant analysis (SDA). Pearson’s correlation coefficient was used to evaluate linear correlation between the study parameters. The results of the ANOVA showed the presence of statistically significant differences (P < 0.01) among half-sib lines for all study traits. The differences within half-sib lines, observed through the contribution of the examined sources of variation to the total variance (%), had higher impact on total variation in the majority of the examined traits. Pearson’s correlation analysis and PCA showed strong relationships between gas exchange in plants and leaf size and stomatal density, as well as between leaf biomass accumulation, intercellular CO2concentration and parameters related to antioxidant capacity of plants. Likewise, the results of SDA indicate that transpiration and stomatal conductance contributed to the largest extent, to the discrimination of the wild cherry half-sib lines. In addition, PCA and CDA showed separation of the wild cherry half-sib lines along the first principal component and first canonical variable with regards to humidity of their original sites. Multiple adaptive differences between the wild cherry half-sib lines indicate high potential of the species to adapt rapidly to climate change. The existence of substantial genetic variability among the wild cherry half-sib lines highlights their potential as genetic resources for reforestation purposes and breeding programmes.
Drug use, harm-reduction practices and attitudes toward the utilisation of drug safety testing services in an Irish cohort of festival-goers
Authors: Ivers Jo-Hanna , Killeen Nicki , Keenan Eamon
Published: September 2021

Background

Festival drug-related deaths are a growing public health concern.

Aim

To examine drug use and related harm-reduction practices and attitudes towards utilisation of drug safety testing services.

Methods

Data collection took place over the 2019 festival season (June–October). The questionnaire was self-reported. Data was gathered via the online survey, which was promoted through online and social media platforms and outlets. Social media communication methods were used to reach the targeted population more effectively.

Results

A total of 1193 Irish festival attendees over the age of 18 completed an anonymous online survey. Alcohol, MDMA powder/crystals, ecstasy pills and cocaine were the highest reported drugs used by Irish festival attendees. The vast majority of participants reported polysubstance use (86.8%/n = 1036). Forty percent of participants (39.98%/n = 477) reported having had sex following the use of a drug at a festival; of these, 66% (n = 316) said that the sex was unprotected. Most participants (84.0%/n = 1003) engaged in some form of harm reduction when taking drugs at festivals.

Overwhelmingly, participants reported a willingness to engage with drug-checking services. The vast majority (96.3%; n = 1149) and would use drug checking services more than three-quarters (75.1%/n = 897) reported that they would use an ‘amnesty bin’ for drugs if it were part of an alert system to notify if dangerous drugs are in circulation. A chi-square test of Independence was conducted to examine whether age and utilisation of drug safety testing service a festival were independent. Moreover, when all cases are taken together, the difference between testing modalities (onsite, offsite and amnesty bin) shows a significant difference p < 001 between those who would use onsite and offsite drug testing facilities.

Conclusion

The evidence from this survey indicates that those young people who use drugs at festivals would be prepared to utilise drug checking services and amnesty bins should help inform the public health response to this important area.
Interaction of microRNAs with sphingosine kinases, sphingosine-1 phosphate, and sphingosine-1 phosphate receptors in cancer
Authors: Xu Guangmeng , Yang Zecheng , Sun Yamin , Dong Hongmei , Ma Jingru
Published: September 2021

Sphingosine-1-phosphate (S1P), a pleiotropic lipid mediator, participates in various cellular processes during tumorigenesis, including cell proliferation, survival, drug resistance, metastasis, and angiogenesis. S1P is formed by two sphingosine kinases (SphKs), SphK1 and SphK2. The intracellularly produced S1P is delivered to the extracellular space by ATP-binding cassette (ABC) transporters and spinster homolog 2 (SPNS2), where it binds to five transmembrane G protein-coupled receptors to mediate its oncogenic functions (S1PR1-S1PR5). MicroRNAs (miRNAs) are small non-coding RNAs, 21–25 nucleotides in length, that play numerous crucial roles in cancer, such as tumor initiation, progression, apoptosis, metastasis, and angiogenesis via binding to the 3′‐untranslated region (3′‐UTR) of the target mRNA. There is growing evidence that various miRNAs modulate tumorigenesis by regulating the expression of SphKs, and S1P receptors. We have reviewed various roles of miRNAs, SphKs, S1P, and S1P receptors (S1PRs) in malignancies and how notable miRNAs like miR-101, miR-125b, miR-128, and miR-506, miR-1246, miR-21, miR-126, miR499a, miR20a-5p, miR-140-5p, miR-224, miR-137, miR-183-5p, miR-194, miR181b, miR136, and miR-675-3p, modulate S1P signaling. These tumorigenesis modulating miRNAs are involved in different cancers including breast, gastric, hepatocellular carcinoma, prostate, colorectal, cervical, ovarian, and lung cancer via cell proliferation, invasion, angiogenesis, apoptosis, metastasis, immune evasion, chemoresistance, and chemosensitivity. Therefore, understanding the interaction of SphKs, S1P, and S1P receptors with miRNAs in human malignancies will lead to better insights for miRNA-based cancer therapy.
Conceptualization and development of multi-layered groundwater model in transient condition
Authors: Omar Padam Jee , Gaur Shishir , Dikshit P. K. S.
Published: September 2021

Effective management of water resource is essential in arid and semi-arid areas of India. In Bihar, for drinking purpose humans, livestock is dependent on the groundwater as well as in agricultural areas groundwater plays an important role in irrigation directly or indirectly. There is rise in the groundwater demand due to rapid population increase and fast industrialization. To meet this groundwater demand, excessive withdrawal of groundwater is a point of concern due to limited storage of it. Assessment of the groundwater was done by preparing a numerical model of the groundwater flow. This model is capable of solving large groundwater problems and associated complexity with it. In this study, a transient multi-layered groundwater flow model was conceptualized and developed for the Koshi River basin. In north Bihar plains, the Koshi River is one of the biggest tributaries of the Ganga River system. Koshi originates from the lower part of Tibet and joins the Ganga River in Katihar district, Bihar, India. After model development, calibration of the model was also done, by considering three model parameters, to represent the actual field conditions. For validation of the model, fifteen observation wells have been selected in the area. With the help of observation well data, computed and observed heads were compared. Comparison results have been found to be encouraging and the computed groundwater head matched with the observed water head to a realistic level of accuracy. Developed groundwater model is used to predict the groundwater head and flow budget in the concerned area. The study revealed that groundwater modeling is an important method for knowing the behavior of aquifer systems and to detect groundwater head under different varying hydrological stresses. This type of study will be beneficial for the hydrologist and water resource engineers to predict the groundwater flow behavior, before implementing any project or to implement a correction scheme.
A study on various pollutants in water and their effect on blood of the consumers
Authors: Shah Syed Faisal Hassan , Shah Syed Hammad Hassan , Ahmad Liaquat , Sherwani Rehan Ahmad Khan , Aslam Muhammad
Published: September 2021

Water pollution is one of the most dangerous problems which is commonly ignored in our country. Pakistan ranks very low in the world, regarding drinkable water. Both surface and underground water are contaminated with toxic metals and pesticides and arsenic (μg/L) were found to be 50. The quality parameters set by the WHO are persistently violated. The water quality is seriously affected by improper disposal of industrial, domestic waste and agrochemicals. These pollutants are responsible alone, or along with other factors for a variety of health problems. This article discusses drinking water being supplied in Sahiwal (Pakistan) to students living in the hostel along with an emphasis on the major pollutants, their effects and consequent health problems. The data presented in this article have been collected in Sahiwal. The water samples were collected from the main supply and the blood samples were of the consumers of that water.
Motif-h: a novel functional backbone extraction for directed networks
Authors: Bai Yiguang , Li Qian , Fan Yanni , Liu Sanyang
Published: September 2021

Dense networks are very pervasive in social analytics, biometrics, communication, architecture, etc. Analyzing and visualizing such large-scale networks are significant challenges, which are generally met by reducing the redundancy on the level of nodes or edges. Motifs, patterns of the higher order organization compared with nodes and edges, are recently found to be the novel fundamental unit structures of complex networks. In this work, we proposed a novel motif h-backbone (Motif-h) method to extract functional cores of directed networks based on both motif strength and h-bridge. Compared with the state-of-the-art method Motif-DF and Entropy, our method solves two main issues which are often found in existing methods: the Motif-h reconsiders weak ties into our candidate set, and those weak ties often have critical functions of bridges in networks; moreover, our method provides a trade-off between the motif size and the edge strength, which quantifies the core edges accordingly. In the simulations, we compare our method with Motif-DF in four real-world networks and found that Motif-h can streamline the extraction of crucial structures compared with the others with limited edges.
A Cautionary Note on Predicting Social Judgments from Faces with Deep Neural Networks
Authors: Keles Umit , Lin Chujun , Adolphs Ralph
Published: September 2021

People spontaneously infer other people’s psychology from faces, encompassing inferences of their affective states, cognitive states, and stable traits such as personality. These judgments are known to be often invalid, but nonetheless bias many social decisions. Their importance and ubiquity have made them popular targets for automated prediction using deep convolutional neural networks (DCNNs). Here, we investigated the applicability of this approach: how well does it generalize, and what biases does it introduce? We compared three distinct sets of features (from a face identification DCNN, an object recognition DCNN, and using facial geometry), and tested their prediction across multiple out-of-sample datasets. Across judgments and datasets, features from both pre-trained DCNNs provided better predictions than did facial geometry. However, predictions using object recognition DCNN features were not robust to superficial cues (e.g., color and hair style). Importantly, predictions using face identification DCNN features were not specific: models trained to predict one social judgment (e.g., trustworthiness) also significantly predicted other social judgments (e.g., femininity and criminal), and at an even higher accuracy in some cases than predicting the judgment of interest (e.g., trustworthiness). Models trained to predict affective states (e.g., happy) also significantly predicted judgments of stable traits (e.g., sociable), and vice versa. Our analysis pipeline not only provides a flexible and efficient framework for predicting affective and social judgments from faces but also highlights the dangers of such automated predictions: correlated but unintended judgments can drive the predictions of the intended judgments.
Predicting if Lung Cancer Will Relapse–The Role of Neutrophil/Lymphocyte Ratio
Authors: Chan Abigail , Bentzen Søren , Rout Amit , Miller Kenneth
Published: September 2021

Objective/Background:

Baseline neutrophil/lymphocyte ratio (NLR), a surrogate marker for systemic inflammation and immunosuppression, is a well-studied prognostic marker in non-small cell lung cancer (NSCLC). This study tests if interim NLR is prognostic in NSCLC patients in remission.

Methods:

This single-center, retrospective cohort study analyzed 131 NSCLC patients treated from 2010 to 2015 who achieved complete remission. NLR was calculated at baseline and from the first available blood sample during remission. Kaplan–Meier estimates of overall survival (OS) and time to recurrence were compared using the log-rank test for trend. Multivariable analysis was conducted using the Cox proportional hazards model.

Results:

Of the 131 cases, 63 had subsequently recurred at the last follow-up. The mean age was 64 ± 10 years. Patients with stage I (35%), II (24%), and III (41%) were included. Histology were adenocarcinoma (60%), squamous cell (33%), and unspecified (7%). The majority of patients were smokers. For the univariate analysis interim NLR was binned into tertiles, NLR < 2, 2–4.08, and > 4.08. Of those with an interim NLR > 4.08, prognosis and recurrence risk were higher. In the multivariable analysis, remission NLR was strongly prognostic for OS (p < .001) as did patient’s age (p = .002), but not stage, race, sex, and baseline NLR.

Conclusions:

Our study found that interim NLR, obtained in remission, was strongly prognostic for OS and recurrence.
“It has to be designed in a way that really challenges people’s assumptions”: preparing scholars to build equitable community research partnerships
Authors: Tang Yan Catalina , Haque Samiya , Chassler Deborah , Lobb Rebecca , Battaglia Tracy , Sprague Martinez Linda
Published: September 2021

Introduction:

Clinical and Translational Award (CTSA) programs are developing relevant training for researchers and community stakeholders participating in community-engaged research (CEnR). However, there is limited research exploring the ways community stakeholders and partners with key CEnR experiences can inform and shape training priorities for prospective CEnR scholars to build meaningful and equitable partnerships.

Methods:

This study conducted and analyzed online individual semi-structured in-depth interviews with community stakeholders (n = 13) engaged in CEnR to identify training priorities for graduate students and emerging scholars.

Findings:

Thematic analysis of 13 interview transcripts revealed four major training priorities for prospective scholars interested in engaging in CEnR: 1) researcher’s positionality, 2) equitable power sharing, 3) funding, and 4) ethics.

Conclusion:

Building equitable research partnerships was a central theme woven across all four training priorities. Further research should focus on examining the development, implementation, and evaluation of CEnR training in partnership with community stakeholders and partners with relevant CEnR experience. Adopting a collaborative approach to incorporate both community stakeholders and researchers’ priorities can align training competencies to better prepare scholars to engage in building research partnerships.
FIGARO simulation: FIlaments & GAlactic RadiO simulation
Authors: Hodgson Torrance , Vazza Franco , Johnston-Hollitt Melanie , McKinley Benjamin
Published: September 2021

We produce the first low to mid-frequency radio simulation that incorporates both traditional extragalactic radio sources as well as synchrotron cosmic web emission. The FIlaments & GAlactic RadiO (FIGARO) simulation includes 10 unique 4° × 4° fields, incorporating active galactic nucleii (AGNs), star-forming galaxies (SFGs), and synchrotron cosmic web emission out to a redshift ofz= 0.8 and over the frequency range 100–1 400 MHz. To do this, the simulation brings together a recent 1003Mpc3magnetohydrodynamic simulation (Vazza et al. 2019, A&A, 627, A5), calibrated to match observed radio relic population statistics, alongside updated ‘T-RECS’ code for simulating extragalactic radio sources (Bonaldi et al. 2019, MNRAS, 482, 2). Uniquely, the AGNs and SFGs are populated and positioned in accordance with the underlying matter density of the cosmological simulation. In this way, the simulation provides an accurate understanding of the apparent morphology, angular scales, and brightness of the cosmic web as well as—crucially—the clustering properties of the cosmic web with respect to the embedded extragalactic radio population. We find that the synchrotron cosmic web does not closely trace the underlying mass distribution of the cosmic web, but is instead dominated by shocked shells of emission surrounding dark matter halos and resembles a large, undetected population of radio relics. We also show that, with accurate kernels, the cosmic web radio emission is clearly detectable by cross-correlation techniques and this signal is separable from the embedded extragalactic radio population. We offer the simulation as a public resource towards the development of techniques for detecting and measuring the synchrotron cosmic web.
Does body size influence mating success? A morphometric study of two Anastrepha (Diptera: Tephritidae) fruit fly species
Authors: Sánchez-Rosario Mayren , Pérez-Staples Diana , Valle-Mora Javier , Sánchez-Guillén Daniel , Ruiz-Montoya Lorena , Liedo Pablo
Published: September 2021

Male mating success depends on various traits and factors, and correctly identifying these traits can be key in the context of pest management. For tephritid pests, controlled through the sterile insect technique (SIT) traits, such as male size, can be manipulated through mass-rearing procedures. Thus, it is particularly important to understand whether male size can favor mating success. Here, we evaluated mating success of males of different sizes in Anastrepha ludens and Anastrepha obliqua, two species controlled through SIT. For both species, a morphometric analysis was performed of mated and unmated mass-reared and wild males in field cages. In both A. ludens and A. obliqua, wild females did not discriminate their mates based on male size and mated more frequently with wild males regardless of size. For mass-reared males, we found no evidence of an advantage of the large males compared to small males in mating success. However, we did find differences between the morphometric traits of mass-reared and wild males. In A. ludens, traits associated to mating success were Face Width (FW), Head Width (HW), Thorax Length (TL) and Wing Length (WL), and for A. obliqua were FW, HW, WL and WW (Wing Width). Overall, FW and TL were more consistent predictors of mating success. In conclusion, female choice seems to suggest multivariate selection, confirming that overall body size (expressed as pupal diameter, which is highly correlated with weight) is not a decisive factor in male mating success in these two species. However, morphological traits such as FW, HW, TL, WL and WW may be relevant in mating preference of wild female.
THE SHAPE OF EARLY GREEK UTOPIA
Authors: Napoli Davide
Published: September 2021

The paper offers a new approach to utopia in early and classical Greek texts from Homer to the fifth century. The model is based on four motifs regularly occurring in ‘utopian texts’, that is, descriptions of places that are distant in time and/or space. A comparative analysis of such texts (drawn from Homer, Hesiod, Pindar, Old Comedy and Herodotus) and of how they manipulate the four motifs sheds new light on specific problems (as, for example, the relevance of Herodotus’ Ethiopian episode, or the role of the myth of Perseus in Pindar's Pythian 10) and encourages more nuanced readings of famous texts, such as Homer's account of Scheria.
Autonomous landing of a quadrotor on a moving platform using vision-based FOFPID control
Authors: Ghasemi Ali , Parivash Farhad , Ebrahimian Serajeddin
Published: September 2021

This research deals with the autonomous landing maneuver of a quadrotor unmanned aerial vehicle (UAV) on an unmanned ground vehicle (UGV). It is assumed that the UGV moves independently, and there is no communication and collaboration between the two vehicles. This paper aims at the design of a closed-loop vision-based control system for quadrotor UAV to perform autonomous landing maneuvers in the possible minimum time despite the wind-induced disturbance force. In this way, a fractional-order fuzzy proportional-integral-derivative controller is introduced for the nonlinear under-actuated system of a quadrotor. Also, a feedback linearization term is included in the control law to compensate model nonlinearities. A supervisory control algorithm is proposed as an autonomous landing path generator to perform fast, smooth, and accurate landings. On the other hand, a compound AprilTag fiducial marker is employed as the target of a vision positioning system, enabling high precision relative positioning in the range between 10 and 350 cm height. A software-in-the-loop simulation testbed is realized on the windows platform. Numerical simulations with the proposed control system are carried out, while the quadrotor system is exposed to different disturbance conditions and actuator dynamics with saturated thrust output are considered.
Making space for degenerate thinking: revaluing architecture with Friedrich Nietzsche
Authors: Weir Simon , Hill Glen
Published: September 2021

Scholarly analysis of the writings on architecture of philosopher Friedrich Nietzsche (1844–1900) has largely focused on passages in Twilight of the Idols where he claims that ‘Architecture is a kind of eloquence of power in forms – now persuading, even flattering, now only commanding.’1 Yet, considering Nietzsche’s theory of the will-to-power – that an innate drive towards power, might, and self-overcoming is the dominant force of existence – architecture gets interpreted in this passage as he would likely have interpreted sculpture. Any recognition of the social, political, physical, and psychological accommodations of architecture are absent. However, in a passage in Joyful Wisdom entitled ‘Architecture for the Perceptive’, Nietzsche wrote of architecture as a carefully crafted space to inhabit. This discussion of architecture as a lived space has received considerably less attention.
Individuals at increased risk for development of bipolar disorder display structural alterations similar to people with manifest disease
Authors: Mikolas Pavol , Bröckel Kyra , Vogelbacher Christoph , Müller Dirk K. , Marxen Michael , Berndt Christina , Sauer Cathrin , Jung Stine , Fröhner Juliane Hilde , Fallgatter Andreas J. , Ethofer Thomas , Rau Anne , Kircher Tilo , Falkenberg Irina , Lambert Martin , Kraft Vivien , Leopold Karolina , Bechdolf Andreas , Reif Andreas , Matura Silke , Stamm Thomas , Bermpohl Felix , Fiebig Jana , Juckel Georg , Flasbeck Vera , Correll Christoph U. , Ritter Philipp , Bauer Michael , Jansen Andreas , Pfennig Andrea
Published: September 2021

In psychiatry, there has been a growing focus on identifying at-risk populations. For schizophrenia, these efforts have led to the development of early recognition and intervention measures. Despite a similar disease burden, the populations at risk of bipolar disorder have not been sufficiently characterized. Within the BipoLife consortium, we used magnetic resonance imaging (MRI) data from a multicenter study to assess structural gray matter alterations inN = 263 help-seeking individuals from seven study sites. We defined the risk using the EPIbipolarassessment tool as no-risk, low-risk, and high-risk and used a region-of-interest approach (ROI) based on the results of two large-scale multicenter studies of bipolar disorder by the ENIGMA working group. We detected significant differences in the thickness of the left pars opercularis (Cohen’sd = 0.47,p = 0.024) between groups. The cortex was significantly thinner in high-risk individuals compared to those in the no-risk group (p = 0.011). We detected no differences in the hippocampal volume. Exploratory analyses revealed no significant differences in other cortical or subcortical regions. The thinner cortex in help-seeking individuals at risk of bipolar disorder is in line with previous findings in patients with the established disorder and corresponds to the region of the highest effect size in the ENIGMA study of cortical alterations. Structural alterations in prefrontal cortex might be a trait marker of bipolar risk. This is the largest structural MRI study of help-seeking individuals at increased risk of bipolar disorder.
LncRNA PCBP1-AS1-mediated AR/AR-V7 deubiquitination enhances prostate cancer enzalutamide resistance
Authors: Zhang Boya , Zhang Mingpeng , Shen Chunyi , Liu Guancong , Zhang Fan , Hou Jingyu , Yao Weitao
Published: September 2021

The refractory of castration-resistant prostate cancer (CRPC) is mainly reflected in drug resistance. The current research on the resistance mechanism of CRPC is still in its infancy. In this study, we revealed for the first time the key role of LncRNA PCBP1-AS1 in CRPC drug resistance. Through detailed in vivo and in vitro studies, we found that PCBP1-AS1 may enhance the deubiquitination of AR/AR-V7 by stabilizing the USP22-AR/AR-V7 complex, thereby preventing AR/AR-V7 from being degraded through the ubiquitin–proteasome pathway. Targeting PCBP1-AS1 can significantly restore the drug sensitivity of enzalutamide-resistant tumors in vivo and in vitro. Our research further expands the function of LncRNA in castration-resistant prostate cancer, which may provide new potential for clinical diagnosis and targeted therapy.
Computational models of category-selective brain regions enable high-throughput tests of selectivity
Authors: Ratan Murty N. Apurva , Bashivan Pouya , Abate Alex , DiCarlo James J. , Kanwisher Nancy
Published: September 2021

Cortical regions apparently selective to faces, places, and bodies have provided important evidence for domain-specific theories of human cognition, development, and evolution. But claims of category selectivity are not quantitatively precise and remain vulnerable to empirical refutation. Here we develop artificial neural network-based encoding models that accurately predict the response to novel images in the fusiform face area, parahippocampal place area, and extrastriate body area, outperforming descriptive models and experts. We use these models to subject claims of category selectivity to strong tests, by screening for and synthesizing images predicted to produce high responses. We find that these high-response-predicted images are all unambiguous members of the hypothesized preferred category for each region. These results provide accurate, image-computable encoding models of each category-selective region, strengthen evidence for domain specificity in the brain, and point the way for future research characterizing the functional organization of the brain with unprecedented computational precision.
Massive methane fluxing from magma–sediment interaction in the end-Triassic Central Atlantic Magmatic Province
Authors: Capriolo Manfredo , Marzoli Andrea , Aradi László E. , Ackerson Michael R. , Bartoli Omar , Callegaro Sara , Dal Corso Jacopo , Ernesto Marcia , Gouvêa Vasconcellos Eleonora M. , De Min Angelo , Newton Robert J. , Szabó Csaba
Published: September 2021

Exceptional magmatic events coincided with the largest mass extinctions throughout Earth’s history. Extensive degassing from organic-rich sediments intruded by magmas is a possible driver of the catastrophic environmental changes, which triggered the biotic crises. One of Earth’s largest magmatic events is represented by the Central Atlantic Magmatic Province, which was synchronous with the end-Triassic mass extinction. Here, we show direct evidence for the presence in basaltic magmas of methane, generated or remobilized from the host sedimentary sequence during the emplacement of this Large Igneous Province. Abundant methane-rich fluid inclusions were entrapped within quartz at the end of magmatic crystallization in voluminous (about 1.0 × 106 km3) intrusions in Brazilian Amazonia, indicating a massive (about 7.2 × 103 Gt) fluxing of methane. These micrometre-sized imperfections in quartz crystals attest an extensive release of methane from magma–sediment interaction, which likely contributed to the global climate changes responsible for the end-Triassic mass extinction.
Isonicotinylation is a histone mark induced by the anti-tuberculosis first-line drug isoniazid
Authors: Jiang Yuhan , Li Yixiao , Liu Cheng , Zhang Lei , Lv Danyu , Weng Yejing , Cheng Zhongyi , Chen Xiangmei , Zhan Jun , Zhang Hongquan
Published: September 2021

Isoniazid (INH) is a first-line anti-tuberculosis drug used for nearly 70 years. However, the mechanism underlying the side effects of INH has remained elusive. Here, we report that INH and its metabolites induce a post-translational modification (PTM) of histones, lysine isonicotinylation (Kinic), also called 4-picolinylation, in cells and mice. INH promotes the biosynthesis of isonicotinyl-CoA (Inic-CoA), a co-factor of intracellular isonicotinylation. Mass spectrometry reveals 26 Kinic sites in histones in HepG2 cells. Acetyltransferases CREB-binding protein (CBP) and P300 catalyse histone Kinic, while histone deacetylase HDAC3 functions as a deisonicotinylase. Notably, MNase sensitivity assay and RNA-seq analysis show that histone Kinic relaxes chromatin structure and promotes gene transcription. INH-mediated histone Kinic upregulates PIK3R1 gene expression and activates the PI3K/Akt/mTOR signalling pathway in liver cancer cells, linking INH to tumourigenicity in the liver. We demonstrate that Kinic is a histone acylation mark with a pyridine ring, which may have broad biological effects. Therefore, INH-induced isonicotinylation potentially accounts for the side effects in patients taking INH long-term for anti-tuberculosis therapy, and this modification may increase the risk of cancer in humans.
Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
Authors: Funken Dominik , Yu Yi , Feng Xiaoyan , Imvised Tawan , Gueler Faikah , Prinz Immo , Madadi-Sanjani Omid , Ure Benno M. , Kuebler Jochen F. , Klemann Christian
Published: September 2021

T-cells have been demonstrated to modulate ischemia–reperfusion injury (IRI) in the kidney, lung, liver, and intestine. Whereas most T-cell subpopulations contribute primarily to the antigen-specific effector and memory phases of immunity, γδ-T-cells combine adaptive features with rapid, innate-like responses that can place them in the initiation phase of immune reactions. Therefore, we aimed to clarify the role of γδ-T-cells in intestinal IRI. Adult wild-type (WT) and γδ-T-cell-deficient mice were subjected to acute intestinal IRI. Gene expression of pro-inflammatory cytokines and influx of leukocyte subpopulations in the gut were assessed by qPCR and flow cytometry. Serum transaminases were measured as an indicator of distant organ IRI. Intestinal IRI led to increased influx of neutrophils, pro-inflammatory cytokine expression and LDH/ALT/AST elevation. Selective deficiency of γδ-T-cells significantly decreased pro-inflammatory cytokine levels and neutrophil infiltration in the gut following IRI compared to controls. Furthermore, γδ-T-cell deficiency resulted in decreased LDH and transaminases levels in sera, indicating amelioration of distant organ injury. Increasing evidence demonstrates a key role of T-cell subpopulations in IRI. We demonstrate that γδ-T-cell deficiency ameliorated pro-inflammatory cytokine production, neutrophil recruitment and distant organ injury. Thus, γδ-T-cells may be considered as mediators contributing to the inflammatory response in the acute phase of intestinal IRI.
Investigation of progression pattern and associated risk factors in glaucoma patients with initial paracentral scotomas using Humphrey 10-2
Authors: Kim Eun Kyoung , Park Hae-Young Lopilly , Hong Kyung Euy , Shin Da Young , Park Chan Kee
Published: September 2021

Central visual field (VF) progression could directly threaten patientss visual function compared to glaucomatous damage. This study was designed to investigate visual field (VF) progression pattern and associated risk factors including optical coherence topography angiographic (OCT-A) findings in glaucoma patients with initial paracentral scotoma. This prospective, observational study included 122 eyes presenting as initial paracentral scotomas with serial 24-2 and 10-2 VF tests at the glaucoma clinic of Seoul St Mary's Hospital between November 2017 and August 2020. The participants underwent at least 5 serial VF exams and OCT-A at baseline. Numerical values of the initial and final 10-2 VF tests were averaged for each VF test point using the total deviation map. Innermost 10-2 VF progression was defined as three or more new contiguous points at the central 12 points on 10-2 VF. Other clinical characteristics were collected including history of disc hemorrhage and vessel density (VD) was measured from OCT-A images. Linear regression analysis was performed to obtain the change of mean deviation and a cut-off for progression was defined for both 24-2 and 10-2 VFs. The average total deviation maps of the initial 10-2 VF tests shows initial paracentral scotoma located in the superior region in an arcuate pattern that was deep in the 4°–6° region above fixation. This arcuate pattern was more broadly located in the 4°–10° region in the primary open-angle glaucoma (POAG) group, while it was closer to fixation in 0°–4° region in the normal-tension glaucoma (NTG) group. The final average map shows deepening of scotomas in the 4°–10° region in POAG, which deepened closer to the region of fixation in NTG. The diagnosis of NTG (β 1.892; 95% CI 1.225–2.516; P = 0.035) and lower choroidal VD in the peripapillary atrophy (PPA) region (β 0.985; 95% CI 0.975 to 0.995; P = 0.022) were significantly related to innermost 10-2 VF progression. Initial paracentral scotomas in NTG tended to progress closer to the region of fixation, which should be monitored closely. Important progression risk factors related to paracentral scotoma near the fixation were the diagnosis of NTG and reduced choroidal VD in the β-zone PPA region using OCT-A. We should consider vascular risk factors in NTG patients presenting with initial paracentral scotoma to avoid vision threatening progression of glaucoma.
Silence and reduced echolocation during flight are associated with social behaviors in male hoary bats (Lasiurus cinereus)
Authors: Corcoran Aaron J. , Weller Theodore J. , Hopkins Annalise , Yovel Yossi
Published: September 2021

Bats are renowned for their sophisticated echolocation. However, recent research has indicated that bats may be less reliant on echolocation than has long been assumed. To test the hypothesis that bats reduce their use of echolocation to avoid eavesdropping by conspecifics, we deployed miniature tags that recorded ultrasound and accelerations on 10 wild hoary bats (Lasiurus cinereus) for one or two nights. This resulted in 997 10-s recordings. Bats switched between periods predominated by their typical high-intensity echolocation, or periods predominated by micro calls (unusually short, quiet calls), or no detectable calls (“silence”). Periods of high-intensity echolocation included high rates of feeding buzzes, whereas periods of micro calls and silence included high rates of social interactions with other bats. Bats switched back to high-intensity echolocation during actual social interactions. These data support the hypothesis that bats use reduced forms of echolocation and fly in silence to avoid eavesdropping from conspecifics, perhaps in the context of mating-related behavior. They also provide the strongest demonstration to date that bats fly for extended periods of time without the use of echolocation.
Postoperative malrotation of humerus shaft fracture causes degeneration of rotator cuff and cartilage
Authors: Wang Cheng , Ma Xiaoyuan , Liu Qiaohui , Dai Guofeng
Published: September 2021

We hypothesized that postoperative malrotation of humeral shaft fractures can alter the bio-mechanical environment of the shoulder; thus, rotator cuff and cartilage degeneration could be induced. Therefore, we designed an animal experiment to evaluate the impact of malrotation deformities after minimally invasive surgery for humeral fractures on the rotator cuff and cartilage, which has rarely been described in previous studies. Twenty-four New Zealand white rabbits were randomly divided into the sham control group (A), negative control group (B) and malrotated group (C). A sham operation with surgical exposure alone was performed in group A. Humeral shaft osteotomy was performed in Group B and C. In Group B, the fractures were fixed in situ with plate -screw system. While in Group C, iatrogenic rotational deformity was created after the proximal end of the fracture being internally rotated by 20 degrees and then subsequently fixed. The animals with bone healing were sacrificed for pathological and biochemical examination. In group C, the modified Mankin scale for cartilage pathology evaluation and the modified Movin scale for tendon both showed highest score among groups with statistical significance (P < 0.05); Disordered alignment and proportion of collagen I/III of rotator cuff were confirmed with picrosirius red staining; Transmission electron microscopy also showed ultrastructural tendon damage. Immunohistochemistry showed that both MMP-1 and MMP-13 expression were significantly higher in group C than groups A and B(P < 0.05). Minimally invasive techniques for humerus shaft fracture might be cosmetically advantageous, but the consequent postoperative malrotation could increase the risk of rotator cuff and cartilage degeneration. This conclusion is supported here by primary evidence from animal experiments.
Utilizing pre-operative MR imaging and adapting optimal needle puncture approach to improve radiofrequency ablation fraction of thoracic dorsal root ganglia
Authors: Li Bing , Huang Yayong , Zhang Yong , Das Sushant Kumar , Zhang Chuan , Li Yang , Xu Xiaoxue , Yang Hanfeng , Du Yong
Published: September 2021

This experimental study evaluates the location of thoracic dorsal root ganglions (DRGs) through magnetic resonance imaging (MRI) scans, and evaluates the radiofrequency ablation (RFA) fraction of different puncture approaches on distinct DRG locations. Eight normal adult corpse specimens were used as thoracic spine specimens. An MRI examination was performed on each specimen using the following MRI sequences: STIR T2WI, fs-FRFSE T2WI, and 3D FIESTA-c. Then thoracic spine specimens (n = 14) were divided into three groups for RFA: Group A, using a transforaminal approach irrespective of DRG location; Group B, using a transforaminal, trans-lateral-zygapophysial or translaminar approach according to the DRG location; and Group C using a combination of puncture approaches. The quality of visualization of thoracic DRGs on STIR T2WI, fs-FRFSE T2WI, and 3D FIESTA-c scans were 53.5% (77/144), 88.2% (127/144), and 93.1% (134/144), respectively. In group A, the RFA fractions of the extraforaminal DRGs (N = 29), intraforaminal DRGs (N = 12) and intraspinal DRGs (N = 7) via a transforaminal approach were 72.6 ± 18.9%, 54.2 ± 24.8% and 32.9 ± 28.1% respectively. In group B, RFA of extraforaminal DRGs via a transforaminal approach (N = 43) or a trans-lateral zygapophysial approach (N = 45) led to ablation fractions of 71.9 ± 15.2% and 72.0 ± 17.9%, respectively; RFA of intraforaminal DRGs via a transforaminal approach (N = 14) or a translaminar approach (N = 16) led to ablation fractions of 57.1 ± 18.0% and 52.5 ± 20.6%, respectively; RFA of intraspinal DRGs via a transforaminal approach (N = 12) or a translaminar approach (N = 14) led to ablation fractions of 34.8 ± 24.6% and 71.8 ± 16.0%, respectively. In group C, the combined approach led to an ablation fraction for extraforaminal DRGs (N = 69) of 82.5 ± 14.1%, for intraforaminal DRGs (N = 39) of 81.5 ± 11.8%, and for intraspinal DRGs (N = 36) of 80.8 ± 13.3%. MRI can accurately assess DRG location before RFA. Adopting different and combined puncturing approaches tailored to different DRG locations can significantly increase the DRG RFA fraction.
Artefact-removal algorithms for Fourier domain quantum optical coherence tomography
Authors: Kolenderska Sylwia M. , Szkulmowski Maciej
Published: September 2021

Quantum Optical Coherence Tomography (Q-OCT) is a non-classical equivalent of Optical Coherence Tomography and is able to provide a twofold axial resolution increase and immunity to resolution-degrading dispersion. The main drawback of Q-OCT are artefacts which are additional elements that clutter an A-scan and lead to a complete loss of structural information for multilayered objects. Whereas there are very practical and successful methods for artefact removal in Time-domain Q-OCT, no such scheme has been devised for Fourier-domain Q-OCT (Fd-Q-OCT), although the latter modality—through joint spectrum detection—outputs a lot of useful information on both the system and the imaged object. Here, we propose two algorithms which process a Fd-Q-OCT joint spectrum into an artefact-free A-scan. We present the theoretical background of these algorithms and show their performance on computer-generated data. The limitations of both algorithms with regards to the experimental system and the imaged object are discussed.
Relationship between obstetric history and recurrent urinary infections
Authors: Vanaclocha-Ferrer Cynthia , Padilla-Fernandez Barbara-Yolanda , Marquez-Sanchez Magaly-Teresa , Garcia-Sanchez María-Helena , Rodriguez-Martin María-de-la-O , Hernandez-Navarro Nayra , Domenech-Perez Cristina , Valverde-Martinez Lauro-Sebastián , Flores-Fraile María-Carmen , Huélamo Misericordia Martínez , Nieto-Barbero José-Angel , Miron-Canelo José-Antonio , Garcia-Cenador María-Begoña , Lorenzo-Gomez María-Fernanda
Published: September 2021

Urinary tract infections affect more than 50% of women. 25% derive from recurrent UTI (RUTI). It is not known the relationship between obstetric history and RUTI occurrence. Investigate the relationship between obstetric events and RUTI. Multicenter observational retrospective study. Groups: G.RUTI (n = 294): women with RUTI; G.NON.RUTI (n = 126): women without RUTI (treated and cured of renal cancer). Descriptive statistics, ANOVA analysis of variance (with Scheffe’s test for normal samples and Kruskal–Wallis for other distributions), Fisher's exact test, Pearson and Spearman correlation studies, and multivariate analysis multiple regression were used. Mean age 61.04 years (19–92), G.RUTI: 56.77 years SD 4.46 (19–85). G.NON.RUTI: 71 years SD 6.73 (25–92) (p = 0.0001). Obstetric history: Nulliparous G.RUTI: 20 (3.4%) G.NON.RUTI: 90 (71.42%) p 0.0001; Eutocic G.RUTI: 416 (70.74%) G.NON.RUTI: 30 (23.8%) p 0.0001. Dystocic G.RUTI: 58 (9.86%) G.NON.RUTI: 56 (44.44%) p 0.0001. G.RUTI abortion: 102 (17.34%) G.NON.RUTI: 30 (23.8%) p 0.1381. Hysterectomy without adnexectomy G.RUTI: 100 (17%) G.NON.RUTI: 18 (14.28%) p 0.5640. Hysterectomy with adnexectomy G.RUTI: 100 (17%) G.NON.RUTI: 66 (52.28%) p 0.0001. Nulliparity, dystocic delivery, and hysterectomy with adnexectomy are more frequent in women without RUTI, while eutocic births are more associated with RUTI. The most prevalent gynaecological-obstetric history in women with RUTI is eutocic delivery associated with a good health state.
The immunotherapy candidate TNFSF4 may help the induction of a promising immunological response in breast carcinomas
Authors: Li Kai , Ma Lei , Sun Ye , Li Xiang , Ren Hong , Tang Shou-Ching , Sun Xin
Published: September 2021

Immune checkpoint blockade, an immunotherapy, has been applied in multiple systemic malignancies and has improved overall survival to a relatively great extent; whether it can be applied in breast cancer remains unknown. We endeavored to explore possible factors that may influence immunotherapy outcomes in breast cancer using several public databases. The possible treatment target TNF superfamily member 4 (TNFSF4) was selected from many candidates based on its abnormal expression profile, survival-associated status, and ability to predict immune system reactions. For the first time, we identified the oncogenic features of TNFSF4 in breast carcinoma. TNFSF4 was revealed to be closely related to treatment that induced antitumor immunity and to interact with multiple immune effector molecules and T cell signatures, which was independent of endocrine status and has not been reported previously. Moreover, the potential immunotherapeutic approach of TNFSF4 blockade showed underlying effects on stem cell expansion, which more strongly and specifically demonstrated the potential effects of applying TNFSF4 blockade-based immunotherapies in breast carcinomas. We identified potential targets that may contribute to breast cancer therapies through clinical analysis and real-world review and provided one potential but crucial tool for treating breast carcinoma that showed effects across subtypes and long-term effectiveness.