bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the Macro-Economic Benefit of Installing a Farm of Oscillating Water Columns in Scotland and Portugal
Authors: Draycott Samuel , Szadkowska Iwona , Silva Marta , Ingram David M
Published: October 2018

The nascent wave energy sector has the potential to contribute significantly to global renewables targets, yet at present there are no proven commercially viable technologies. Macro-economic assessment is seldom used to assess wave energy projects, yet can provide insightful information on the wider economic benefits and can be used in conjunction with techno-economic analysis to inform policy makers, investors and funding bodies. Herein, we present a coupled techno–macro-economic model, which is used to assess the macro-economic benefit of installing a 5.25 MW farm of oscillating water column wave energy devices at two locations: Orkney in Scotland and Leixoes in Portugal. Through an input-output analysis, the wide-reaching macro-economic benefit of the prospective projects is highlighted; evidenced by the finding that all 29 industry sectors considered are either directly or indirectly stimulated by the project for both locations. Peak annual employment is expected to be 420 and 190 jobs in Portugal and Scotland respectively during the combined installation and manufacturing stage, with an associated peak annual GVA of over €16.6 m and €12.8 m. The discrepancies between the two locations is concluded to largely be a result of the site-specific attributes of the farm locations: specifically, increased water depth and distance to shore for the Portuguese site, resulting in higher costs associated with mooring and electrical cables and vessels. The insights gained through the presented results demonstrate the merit of macro-economic analysis for understanding the wider economic benefit of wave energy projects, while providing an understanding over key physical factors which will dominate estimated effects.
Hydrogen as a Long-Term Large-Scale Energy Storage Solution to Support Renewables
Authors: Kharel Subodh , Shabani Bahman
Published: October 2018

This paper presents a case study of using hydrogen for large-scale long-term storage application to support the current electricity generation mix of South Australia state in Australia, which primarily includes gas, wind and solar. For this purpose two cases of battery energy storage and hybrid battery-hydrogen storage systems to support solar and wind energy inputs were compared from a techno-economical point of view. Hybrid battery-hydrogen storage system was found to be more cost competitive with unit cost of electricity at $0.626/kWh (US dollar) compared to battery-only energy storage systems with a $2.68/kWh unit cost of electricity. This research also found that the excess stored hydrogen can be further utilised to generate extra electricity. Further utilisation of generated electricity can be incorporated to meet the load demand by either decreasing the base load supply from gas in the present scenario or exporting it to neighbouring states to enhance economic viability of the system. The use of excess stored hydrogen to generate extra electricity further reduced the cost to $0.494/kWh.
Energy Efficiency Analysis Carried Out by Installing District Heating on a University Campus. A Case Study in Spain
Authors: Marina Domingo Ana M. , Rey-Hernández Javier M. , San José Alonso Julio F. , Mata Crespo Raquel , Rey Martínez Francisco J.
Published: October 2018

This article analyses the reduction of energy consumption following the installation of district heating (DH) in the Miguel Delibes campus at the University of Valladolid (Spain), in terms of historical consumption and climate variables data. In order to achieve this goal, consumption models are carried out for each building, enabling the comparison of actual data with those foreseen in the model. This paper shows the statistical method used to accept these models, selecting the most influential climate variables data obtained by the models from the consumption baselines in the buildings at the Miguel Delibes campus through to the linear regression equations with a confidence level of 95%. This study shows that the best variables correlated with consumption are the degree-days for 58% of buildings and the average temperature for the remaining 42%. The savings obtained to date with this third generation network have been significantly higher than the 21% average for 33% of the campus buildings. In the case of 17% of the buildings, there was a significant increase in consumption of 20%, and in the case of the remaining 50% of the buildings, no significant differences were found between consumption before and after installation of district heating.
Wind Power Consumption Research Based on Green Economic Indicators
Authors: Wang Xiuyun , Zhou Yibing , Tian Junyu , Wang Jian , Cui Yang
Published: October 2018

As a representative form of new energy generation, wind power has effectively alleviated environmental pollution and energy shortages. This paper constructs a green economic indicator to measure the degree of coordinated development of environmental and social benefits. To increase the amount of wind power consumption, an economic dispatch model based on the coordinated operation of cogeneration units and electric boilers was established; we also introduced the green certificate transaction cost, which effectively meets the strategic needs of China’s energy low-carbon transformation top-level system design. Wind power output has instability and volatility, so it puts higher requirements on the stable operation of thermal power units. To solve the stability problem, this paper introduces the output index of the thermal power unit and rationally plans the unit combination strategy, as well as introducing the concept of chance-constrained programming due to the uncertainty of load and wind power in the model. Uncertainty factors are transformed into load forecasting errors and wind power prediction errors for processing. Based on the normal distribution theory, the uncertainty model is transformed into a certain equivalence class model, and the improved disturbance mutated particle swarm optimization algorithm is used to solve the problem. Finally, the validity and feasibility of the proposed model are verified based on the IEEE30 node system.
Recent Trends in Large Hardwoods in the Pacific Northwest, USA
Authors: Long Jonathan W. , Gray Andrew , Lake Frank K.
Published: October 2018

Forest densification, wildfires, and disease can reduce the growth and survival of hardwood trees that are important for biological and cultural diversity within the Pacific Northwest of USA. Large, full-crowned hardwoods that produce fruit and that form large cavities used by wildlife were sustained by frequent, low-severity fires prior to Euro-American colonization. Shifts in fire regimes and other threats could be causing declines in, large hardwood trees. To better understand whether and where such declines might be occurring, we evaluated recent trends in Forest Inventory and Analysis (FIA) data from 1991–2016 in California and southern Oregon. We included plots that lay within areas of frequent fire regimes during pre-colonial times and potential forest habitats for fisher, a rare mammal that depends on large live hardwoods. We analyzed changes in basal area for eight hardwood species, both overall and within size classes, over three time periods within ecoregions, and in public and private land ownerships. We found the basal area to generally be stable or increasing for these species. However, data for California black oak suggested a slight decline in basal area overall, and among both very large trees and understory trees; that decline was associated with fire mortality on national forest lands. In addition, mature trees with full crowns appeared to sharply decline across all species. Many trends were not statistically significant due to high variation, especially since more precise data from remeasured trees were only available for the two most recent time periods. Continued analysis of these indicators using remeasured trees will help to evaluate whether conservation efforts are sustaining large, full-crowned trees and their associated benefits.
Belowground Carbohydrate Reserves of Mature Southern Pines Reflect Seedling Strategy to Evolutionary History of Disturbance
Authors: Mims Joshua T. , O’Brien Joseph J. , Aubrey Doug P.
Published: October 2018

Carbohydrate reserves provide advantages for mature trees experiencing frequent disturbances; however, it is unclear if selective pressures operate on this characteristic at the seedling or mature life history stage. We hypothesized that natural selection has favored carbohydrate reserves in species that have an evolutionary history of frequent disturbance and tested this using three southern pine species that have evolved across a continuum of fire frequencies. Longleaf pine (Pinus palustris) roots exhibited higher maximum starch concentrations than slash (P. elliottii) and loblolly (P. taeda), which were similar. Longleaf also relied on starch reserves in roots more than slash or loblolly, depleting 64, 41, and 23 mg g−1 of starch, respectively, between seasonal maximum and minimum, which represented 52%, 45%, and 26% of reserves, respectively. Starch reserves in stems did not differ among species or exhibit temporal dynamics. Our results suggest that an evolutionary history of disturbance partly explains patterns of carbohydrate reserves observed in southern pines. However, similarities between slash and loblolly indicate that carbohydrate reserves do not strictly follow the continuum of disturbance frequencies among southern pine, but rather reflect the different seedling strategies exhibited by longleaf compared to those shared by slash and loblolly. We propose that the increased carbohydrate reserves in mature longleaf may simply be a relic of selective pressures imposed at the juvenile stage that are maintained through development, thus allowing mature trees to be more resilient and to recover from chronic disturbances such as frequent fire.
An Environmentally Aware Scheme of Wireless Sensor Networks for Forest Fire Monitoring and Detection
Authors: Xu Yi-Han , Sun Qiu-Ya , Xiao Yu-Tong
Published: October 2018

Forest fires are a fatal threat to environmental degradation. Wireless sensor networks (WSNs) are regarded as a promising candidate for forest fire monitoring and detection since they enable real-time monitoring and early detection of fire threats in an efficient way. However, compared to conventional surveillance systems, WSNs operate under a set of unique resource constraints, including limitations with respect to transmission range, energy supply and computational capability. Considering that long transmission distance is inevitable in harsh geographical features such as woodland and shrubland, energy-efficient designs of WSNs are crucial for effective forest fire monitoring and detection systems. In this paper, we propose a novel framework that harnesses the benefits of WSNs for forest fire monitoring and detection. The framework employs random deployment, clustered hierarchy network architecture and environmentally aware protocols. The goal is to accurately detect a fire threat as early as possible while maintaining a reasonable energy consumption level. ns-2-based simulation validates that the proposed framework outperforms the conventional schemes in terms of detection delay and energy consumption.
Effect of Density and Fiber Size on Porosity and Thermal Conductivity of Fiberboard Mats
Authors: Rebolledo Pamela , Cloutier Alain , Yemele Martin-Claude
Published: October 2018

The thermal conductivity and porosity of fiberboard mats are crucial parameters for efficient energy consumption of the hot-pressing process and for final panel quality. In this work, the effect of fiber size and mat density on porosity and thermal conductivity of the mat were investigated. The fiber size was characterized as fine, medium and coarse. The mat porosity was measured by image analysis using the black and white contrast method. The thermal conductivity was determined at different density levels with a temperature gradient of 1.6 °C mm−1 and 7.6% (s = 0.3) moisture content. The results showed that fiber size was a dominant variable governing heat conduction and mat porosity. The mats made with medium size fibers showed a higher resistance to compression. The thermal conductivity of coarse fiber mats decreased drastically between 700 kg m−3 and 810 kg m−3. This was likely due to a higher fracture frequency observed for coarse fibers in comparison to the other fiber sizes studied. Hence, the fine and medium fibers conducted heat more efficiently. Moreover, fiber bundles and fractured fibers were observed during the mat porosity measurements, principally in mats made with fine fiber size.
Changes in Lightning Fire Incidence in the Tasmanian Wilderness World Heritage Area, 1980–2016
Authors: Styger Jenny , Marsden-Smedley Jon , Kirkpatrick Jamie
Published: October 2018

The Tasmanian Wilderness World Heritage Area (TWWHA) has globally significant natural and cultural values, some of which are dependent on the absence of fire or the presence of particular fire regimes. Planned burning is currently used to reduce the risk of loss of world heritage values from unplanned fires, but large and damaging fires still occur, with lightning as the primary ignition source. Lightning-caused fire was rare in the TWWHA before 2000. There has since been an increase in both the number of fires following lightning storms and the area burnt by these fires. In the absence of a direct measurement of lightning strike incidence, we tested whether changes in rainfall, soil dryness and fuel load were responsible for these changes in fire incidence and extent. There were no relationships between these variables and the incidence of fires associated with lightning, but the variability in the Soil Dryness Index and the mean of 25% of driest values did predict both the number and area of fires. Thus, it appears that an increase in the proportion of lightning strikes that occur in dry conditions has increased ignition efficiency. These changes have important implications for the management of the TWWHA’s values, as higher projected fuel loads and drier climates could result in a further increase in the number of fires associated with lightning.
Impact of Pre-Processed Chickpea Flour Incorporation into “Mankoushe” on Appetite Hormones and Scores
Authors: Dandachy Sahar , Mawlawi Hiba , Chedid Marwan , El-Mallah Carla , Obeid Omar
Published: October 2018

Recently, there has been an increasing interest in integrating pulse flours into pastries and baked products to improve their nutritional and health benefits. “Mankoushe,” a popular Lebanese pastry made up of refined wheat flour was enriched with chickpea flour that is of better nutritional value, and its postprandial glycemia, insulinemia, lipidemia and appetite measures were monitored. A randomized cross-over study was performed on sixteen healthy Lebanese females, age (years): 22.90 ± 3.00, and BMI (kg/m2): 22.70 ± 2.65. Over-night fasted females were asked to consume two iso-energetic meals (201 g; 681 kcal) on two separate days, three days apart. One meal was the “Regular Mankoushe” (RM) made with white flour 100%, and the second meal was the “Chickpeas Mankoushe” (CM) made with a mixture of wheat/chickpea flour (70/30). Blood samples were collected 15 min before meal ingest and at 30, 90, 150 and 210 min postprandial. Glucose, insulin, triglycerides (TG), ghrelin, and glucagon-like peptide 1 (GLP-1) plasma levels were measured. Subjective appetite rating and food intake were also assessed. Incorporation of pre-processed chickpea flour into “Mankoushe” as 30% of the dough was associated with a modest reduction in both glucose and insulin levels, and TG was minimally affected. At the level of appetite hormones, changes in GLP-1 were similar, whereas the reduction in ghrelin was significantly lower after the RM meal and thus favored a higher satiating effect compared to CM. This was not paralleled by a similar change in subjective appetite scores and subsequent energy intake. In conclusion, findings suggest that pre-processed chickpea flour could be a promising functional ingredient of traditional pastries to improve their nutritional quality. Nevertheless, further investigations are warranted regarding its satiating effect.
Close Binaries and the Abundance Discrepancy Problem in Planetary Nebulae
Authors: Wesson R. , Jones D. , García-Rojas J. , Boffin H. M. J. , Corradi R. L. M.
Published: October 2018

Motivated by the recent establishment of a connection between central star binarity and extreme abundance discrepancies in planetary nebulae, we have carried out a spectroscopic survey targeting planetary nebula with binary central stars and previously unmeasured recombination line abundances. We have discovered seven new extreme abundance discrepancies, confirming that binarity is key to understanding the abundance discrepancy problem. Analysis of all 15 objects with a binary central star and a measured abundance discrepancy suggests a cut-off period of about 1.15 days, below which extreme abundance discrepancies are found.
Accessing Scottish Archives Online
Authors: Pelan John
Published: October 2018

John Pelan, Director of the Scottish Council on Archives (SCA), explores some of the challenges around searching Scotland’s archives online. Difficulties in accessing information, knowing what exists and where to find it, and the multiplicity of online catalogues can be confusing and frustrating for users, particularly inexperienced and amateur family historians. The article provides information about the Scottish Council on Archives (SCA) plans, working in partnership with a wide range of stakeholders, to create a new portal for accessing Scotland’s archive collections including those of universities, local authorities, businesses and communities. The portal, which will be a development of the existing Scottish Archive Network resource, will allow users to search across many catalogues for both collection and item level records. The new portal will be an invaluable resource for genealogists, researchers, academics, students, historians and members of the public by providing guidance on understanding, using and accessing archives. SCA expects that the site will become a powerful advocacy tool for archives, showing not just the breadth and depth of collections across Scotland but highlighting the many ways that archives can be used inform and improve society. For genealogists, but also for everyday users of archives as well as potential new users, this portal will open new channels of research and local history.
Virus Sensor RIG-I Represses RNA Interference by Interacting with TRBP through LGP2 in Mammalian Cells
Authors: Takahashi Tomoko , Nakano Yuko , Onomoto Koji , Yoneyama Mitsutoshi , Ui-Tei Kumiko
Published: October 2018

Exogenous double-stranded RNAs (dsRNAs) similar to viral RNAs induce antiviral RNA silencing or RNA interference (RNAi) in plants or invertebrates, whereas interferon (IFN) response is induced through activation of virus sensor proteins including Toll like receptor 3 (TLR3) or retinoic acid-inducible gene I (RIG-I) like receptors (RLRs) in mammalian cells. Both RNA silencing and IFN response are triggered by dsRNAs. However, the relationship between these two pathways has remained unclear. Laboratory of genetics and physiology 2 (LGP2) is one of the RLRs, but its function has remained unclear. Recently, we reported that LGP2 regulates endogenous microRNA-mediated RNA silencing by interacting with an RNA silencing enhancer, TAR-RNA binding protein (TRBP). Here, we investigated the contribution of other RLRs, RIG-I and melanoma-differentiation-associated gene 5 (MDA5), in the regulation of RNA silencing. We found that RIG-I, but not MDA5, also represses short hairpin RNA (shRNA)-induced RNAi by type-I IFN. Our finding suggests that RIG-I, but not MDA5, interacts with TRBP indirectly through LGP2 to function as an RNAi modulator in mammalian cells.
Whole Genome Resequencing of Arkansas Progressor and Regressor Line Chickens to Identify SNPs Associated with Tumor Regression
Authors: Khatri Bhuwan , Hayden Ashley M. , Anthony Nicholas B. , Kong Byungwhi C.
Published: October 2018

Arkansas Regressor (AR) chickens, unlike Arkansas Progressor (AP) chickens, regress tumors induced by the v-src oncogene. To better understand the genetic factors responsible for this tumor regression property, whole genome resequencing was conducted using Illumina Hi-Seq 2 × 100 bp paired-end read method (San Diego, CA, USA) with AR (confirmed tumor regression property) and AP chickens. Sequence reads were aligned to the chicken reference genome (galgal5) and produced coverage of 11× and 14× in AR and AP, respectively. A total of 7.1 and 7.3 million single nucleotide polymorphisms (SNPs) were present in AR and AP genomes, respectively. Through a series of filtration processes, a total of 12,242 SNPs were identified in AR chickens that were associated with non-synonymous, frameshift, nonsense, no-start and no-stop mutations. Further filtering of SNPs based on read depth ≥ 10, SNP% ≥ 0.75, and non-synonymous mutations identified 63 reliable marker SNPs which were chosen for gene network analysis. The network analysis revealed that the candidate genes identified in AR chickens play roles in networks centered to ubiquitin C (UBC), phosphoinositide 3-kinases (PI3K), and nuclear factor kappa B (NF-kB) complexes suggesting that the tumor regression property in AR chickens might be associated with ubiquitylation, PI3K, and NF-kB signaling pathways. This study provides an insight into genetic factors that could be responsible for the tumor regression property.
Influence of Heat Events on the Composition of Airborne Bacterial Communities in Urban Ecosystems
Authors: Fang Zhiguo , Guo Weijun , Zhang Junwen , Lou Xiuqin
Published: October 2018

Airborne bacteria are significantly affected by meteorological and environmental conditions. However, there is little quantitative data available on the effects of these factors on airborne bacteria in urban ecosystems. In the present study, we analyzed weather-dependent changes in the composition of airborne bacterial communities using high throughput sequencing. Samples were collected before and after a period of constant hot weather at four selected sampling sites (YRBS, ZJGUSJC, TJCR, and BLQG) in Hangzhou. Our results show that the average amount of bacterial 16S rRNA gene copy numbers per m3 of air decreased significantly after constant high temperature. In addition, the number of operational taxonomic units and the Shannon–Wiener diversity indexes of the samples at all four selected sampling sites were significantly decreased after the heat event, showing notable impact on bacterial diversity. We also detected a significant increase in the abundances of spore-forming bacteria. Firmicutes increased from 3.7% to 9.9%, Bacillales increased from 2.6% to 7.6%, and Bacillaceae increased from 1.5% to 5.9%. In addition, we observed an increase in beta-Proteobacteria (18.2% to 50.3%), Rhodocyclaceae (6.9% to 29.9%), and Burkholderiaceae (8.1% to 15.2%). On the other hand, the abundance of alpha-Proteobacteria (39.6% to 9.8%), Caulobacteraceae (17.9% to 0.5%), Sphingomonadaceae (7.2% to 3.3%), and Xanthomonadaceae (3.0% to 0.5%) was significantly lower. Taken together, our data suggest that the composition of airborne bacterial communities varies greatly dependent on heat events, and that such communities include several species that are highly susceptible to high-temperature related stressors such as high air temperature, low relative humidity, and high intensity of solar radiation.
Fruit and Vegetable Consumption among Immigrants in Portugal: A Nationwide Cross-Sectional Study
Authors: Costa Liliane , Dias Sónia , Martins Maria do Rosário O.
Published: October 2018

This study aims to compare adequate fruit and vegetable (F&V) intake between immigrants and natives in Portugal, and to analyse factors associated with consumption of F&V among immigrants. Data from a population based cross-sectional study (2014) was used. The final sample comprised 17,410 participants (≥20 years old), of whom 7.4% were immigrants. Chi-squared tests and logistic regression models were conducted to investigate the association between adequate F&V intake, sociodemographic, anthropometric, and lifestyle characteristics. Adequate F&V intake was more prevalent among immigrants (21.1% (95% CI: 19.0–23.4)) than natives (18.5% (95% CI: 17.9–19.1)), (p = 0.000). Association between migrant status and adequate F&V intake was only evident for men: immigrants were less likely to achieve an adequate F&V intake (OR = 0.67, 95% CI = 0.66–0.68) when compared to Portuguese. Among immigrants, being female, older, with a higher education, and living in a low urbanisation area increased the odds of having F&V consumption closer to the recommendations. Adjusting for other factors, length of residence appears as a risk factor (15 or more years vs. 0–9 years: OR = 0.52, 95% CI = 0.50–0.53), (p = 0.000) for adequate F&V intake. Policies aiming to promote adequate F&V consumption should consider both populations groups, and gender-based strategies should address proper sociodemographic, anthropometric, and lifestyle determinants.
Global and Geographically Weighted Quantile Regression for Modeling the Incident Rate of Children’s Lead Poisoning in Syracuse, NY, USA
Authors: Zhen Zhen , Cao Qianqian , Shao Liyang , Zhang Lianjun
Published: October 2018

Objective: The purpose of this study was to explore the full distribution of children’s lead poisoning and identify “high risk” locations or areas in the neighborhood of the inner city of Syracuse (NY, USA), using quantile regression models. Methods: Global quantile regression (QR) and geographically weighted quantile regression (GWQR) were applied to model the relationships between children’s lead poisoning and three environmental factors at different quantiles (25th, 50th, 75th, and 90th). The response variable was the incident rate of children’s blood lead level ≥ 5 µg/dL in each census block, and the three predictor variables included building year, town taxable values, and soil lead concentration. Results: At each quantile, the regression coefficients of both global QR and GWQR models were (1) negative for both building year and town taxable values, indicating that the incident rate of children lead poisoning reduced with newer buildings and/or higher taxable values of the houses; and (2) positive for the soil lead concentration, implying that higher soil lead concentration around the house may cause higher risks of children’s lead poisoning. Further, these negative or positive relationships between children’s lead poisoning and three environmental factors became stronger for larger quantiles (i.e., higher risks). Conclusions: The GWQR models enabled us to explore the full distribution of children’s lead poisoning and identify “high risk” locations or areas in the neighborhood of the inner city of Syracuse, which would provide useful information to assist the government agencies to make better decisions on where and what the lead hazard treatment should focus on.
Exercise Training Attenuates Obesity-Induced Skeletal Muscle Remodeling and Mitochondria-Mediated Apoptosis in the Skeletal Muscle
Authors: Heo Jun-Won , Yoo Su-Zi , No Mi-Hyun , Park Dong-Ho , Kang Ju-Hee , Kim Tae-Woon , Kim Chang-Ju , Seo Dae-Yun , Han Jin , Yoon Jin-Hwan , Jung Su-Jeen , Kwak Hyo-Bum
Published: October 2018

Obesity is characterized by the induction of skeletal muscle remodeling and mitochondria-mediated apoptosis. Exercise has been reported as a positive regulator of skeletal muscle remodeling and apoptosis. However, the effects of exercise on skeletal muscle remodeling and mitochondria-mediated apoptosis in obese skeletal muscles have not been clearly elucidated. Four-week-old C57BL/6 mice were randomly assigned into four groups: control (CON), control plus exercise (CON + EX), high-fat diet (HFD), and HFD plus exercise groups (HFD + EX). After obesity was induced by 20 weeks of 60% HFD feeding, treadmill exercise was performed for 12 weeks. Exercise ameliorated the obesity-induced increase in extramyocyte space and a decrease in the cross-sectional area of the skeletal muscle. In addition, it protected against increases in mitochondria-mediated apoptosis in obese skeletal muscles. These results suggest that exercise as a protective intervention plays an important role in regulating skeletal muscle structure and apoptosis in obese skeletal muscles.
Relationship between the Main Communities and Environments of an Urban River and Reservoir: Considering Integrated Structural and Functional Assessments of Ecosystems
Authors: Tang Dehao , Liu Xingjian , Wang Xutao , Yin Kedong
Published: October 2018

Rivers and reservoirs in urban areas have been associated with environmental quality problems because of the discharge of domestic waste into water bodies. However, the key effects and the extent to which environmental factors can influence the integrated structure and function of urban river ecosystems remain largely unknown. Here, a relationship model involving the species composition of the community and the various environmental factors related to the water and sediment was developed in the dry season (N) and the flood season (F) in both the urban Jiaomen River (JR) and the Baihuitian Reservoir (BR) of Guangzhou City. Canonical correspondence analysis was used to determine the spatiotemporal drivers of the phytoplankton, zooplankton and macrobenthic communities in the river and reservoir systems. The combination of the thermodynamic-oriented ecological indicators and the biodiversity measures reflected the integrated structure and function of the ecosystems. Overall, the plankton community composition was found to be largely determined by the nutrient concentrations and oxygen index, and the development of the macrobenthic communities was mainly restricted by organic matter and heavy metals. Based on the results of the integrated assessment, the structure and function of the JR ecosystem were superior to that of the BR, and the F period displayed healthier results than the N period. Moreover, the structural and functional statuses of the high eco-exergy grade communities (macrobenthic communities) in the ecosystem influenced the regional changes observed in the results of the integrated assessment. The significant seasonal variations in the plankton community affected the seasonal variations in the integrated assessment. The results of this study provide a scientific basis for the management and restoration of regional freshwater environments and ecosystems.
Climate Change Adaptation through the Water-Energy-Food Nexus in Southern Africa
Authors: Mpandeli Sylvester , Naidoo Dhesigen , Mabhaudhi Tafadzwanashe , Nhemachena Charles , Nhamo Luxon , Liphadzi Stanley , Hlahla Sithabile , Modi Albert T.
Published: October 2018

Climate change is a complex and cross-cutting problem that needs an integrated and transformative systems approach to respond to the challenge. Current sectoral approaches to climate change adaptation initiatives often create imbalances and retard sustainable development. Regional and international literature on climate change adaptation opportunities and challenges applicable to southern Africa from a water-energy-food (WEF) nexus perspective was reviewed. Specifically, this review highlights climate change impacts on water, energy, and food resources in southern Africa, while exploring mitigation and adaptation opportunities. The review further recommends strategies to develop cross-sectoral sustainable measures aimed at building resilient communities. Regional WEF nexus related institutions and legal frameworks were also reviewed to relate the WEF nexus to policy. Southern Africa is witnessing an increased frequency and intensity in climate change-associated extreme weather events, causing water, food, and energy insecurity. A projected reduction of 20% in annual rainfall by 2080 in southern Africa will only increase the regional socio-economic challenges. This is exacerbating regional resource scarcities and vulnerabilities. It will also have direct and indirect impacts on nutrition, human well-being, and health. Reduced agricultural production, lack of access to clean water, sanitation, and clean, sustainable energy are the major areas of concern. The region is already experiencing an upsurge of vector borne diseases (malaria and dengue fever), and water and food-borne diseases (cholera and diarrhoea). What is clear is that climate change impacts are cross-sectoral and multidimensional, and therefore require cross-sectoral mitigation and adaptation approaches. In this regard, a well-coordinated and integrated WEF nexus approach offers opportunities to build resilient systems, harmonise interventions, and mitigate trade-offs and hence improve sustainability. This would be achieved through greater resource mobilisation and coordination, policy convergence across sectors, and targeting nexus points in the landscape. The WEF nexus approach has potential to increase the resilience of marginalised communities in southern Africa by contributing towards attaining the Sustainable Development Goals (SDGs 1, 2, 3, 6, 7, and 13).
Gas Signaling Molecules and Mitochondrial Potassium Channels
Authors: Walewska Agnieszka , Szewczyk Adam , Koprowski Piotr
Published: October 2018

Recently, gaseous signaling molecules, such as carbon monoxide (CO), nitric oxide (NO), and hydrogen sulfide (H2S), which were previously considered to be highly toxic, have been of increasing interest due to their beneficial effects at low concentrations. These so-called gasotransmitters affect many cellular processes, such as apoptosis, proliferation, cytoprotection, oxygen sensing, ATP synthesis, and cellular respiration. It is thought that mitochondria, specifically their respiratory complexes, constitute an important target for these gases. On the other hand, increasing evidence of a cytoprotective role for mitochondrial potassium channels provides motivation for the analysis of the role of gasotransmitters in the regulation of channel function. A number of potassium channels have been shown to exhibit activity within the inner mitochondrial membrane, including ATP-sensitive potassium channels, Ca2+-activated potassium channels, voltage-gated Kv potassium channels, and TWIK-related acid-sensitive K+ channel 3 (TASK-3). The effects of these channels include the regulation of mitochondrial respiration and membrane potential. Additionally, they may modulate the synthesis of reactive oxygen species within mitochondria. The opening of mitochondrial potassium channels is believed to induce cytoprotection, while channel inhibition may facilitate cell death. The molecular mechanisms underlying the action of gasotransmitters are complex. In this review, we focus on the molecular mechanisms underlying the action of H2S, NO, and CO on potassium channels present within mitochondria.
Optical Graphene-Based Biosensor for Nucleic Acid Detection; Influence of Graphene Functionalization and Ionic Strength
Authors: Becheru Diana F. , Vlăsceanu George M. , Banciu Adela , Vasile Eugeniu , Ioniţă Mariana , Burns Jorge S.
Published: October 2018

A main challenge for optical graphene-based biosensors detecting nucleic acid is the selection of key parameters e.g. graphenic chemical structure, nanomaterial dispersion, ionic strength, and appropriate molecular interaction mechanisms. Herein we study interactions between a fluorescein-labelled DNA (FAM-DNA) probe and target single-stranded complementary DNA (cDNA) on three graphenic species, aiming to determine the most suitable platform for nucleic acid detection. Graphene oxide (GO), carboxyl graphene (GO-COOH) and reduced graphene oxide functionalized with PEGylated amino groups (rGO-PEG-NH2, PEG (polyethylene glycol)) were dispersed and characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The influence of ionic strength on molecular interaction with DNA was examined by fluorescence resonance energy transfer (FRET) comparing fluorescence intensity and anisotropy. Results indicated an effect of graphene functionalization, dispersion and concentration-dependent quenching, with GO and GO-COOH having the highest quenching abilities for FAM-DNA. Furthermore, GO and GO-COOH quenching was accentuated by the addition of either MgCl2 or MgSO4 cations. At 10 mM MgCl2 or MgSO4, the cDNA induced a decrease in fluorescence signal that was 2.7-fold for GO, 3.4-fold for GO-COOH and 4.1-fold for rGO-PEG-NH2. Best results, allowing accurate target detection, were observed when selecting rGO-PEG-NH2, MgCl2 and fluorescence anisotropy as an advantageous combination suitable for nucleic acid detection and further rational design biosensor development.
Fullerene Derivatives of Nucleoside HIV Reverse Transcriptase Inhibitors—In Silico Activity Prediction
Authors: Dąbrowska Aleksandra , Pieńko Tomasz , Taciak Przemysław , Wiktorska Katarzyna , Chilmonczyk Zdzisław , Mazurek Aleksander , Stasiulewicz Adam
Published: October 2018

Here we present new derivatives of nucleoside reverse transcriptase inhibitors with a C20 fullerene. The computational chemistry methods used in this study evaluate affinity of designed compounds towards the HIV-1 reverse transcriptase (RT) binding site and select the most active ones. The best of the designed compounds have superior or similar affinity to RT active site in comparison to most active test compounds, including drugs used in anti-HIV therapy.
Emerging Role of Follicular T Helper Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis
Authors: Quinn James L. , Axtell Robert C.
Published: October 2018

Multiple sclerosis (MS) is an autoimmune disorder where both T cells and B cells are implicated in pathology. However, it remains unclear how these two distinct populations cooperate to drive disease. There is ample evidence from studies in both MS patients and mouse models that Th17, B cells, and follicular T helper (TFH) cells contribute to disease. This review article describes the literature that identifies mechanisms by which Th17, TFH, and B cells cooperatively drive disease activity in MS and experimental autoimmune encephalomyelitis (EAE). The curation of this literature has identified that central nervous system (CNS) infiltrating TFH cells act with TH17 cell to contribute to an inflammatory B cell response in neuroinflammation. This demonstrates that TFH cells and their products are promising targets for therapies in MS.
Sorafenib in Combination with Betulinic Acid Synergistically Induces Cell Cycle Arrest and Inhibits Clonogenic Activity in Pancreatic Ductal Adenocarcinoma Cells
Authors: Kutkowska Justyna , Strzadala Leon , Rapak Andrzej
Published: October 2018

Pancreatic ductal adenocarcinoma (PDAC) is one of the most deadly cancers in the world due to late diagnosis and poor response to available treatments. It is important to identify treatment strategies that will increase the efficacy and reduce the toxicity of the currently used therapeutics. In this study, the PDAC cell lines AsPC-1, BxPC-3, and Capan-1 were treated with sorafenib and betulinic acid alone and in combination. We examined the effect of combined treatments on viability (MTS test), proliferation and apoptosis (annexin V staining), cell cycle arrest (PI staining), alterations in signaling pathways (Western blotting), and colony-forming ability. The combination of sorafenib with betulinic acid inhibited the viability and proliferation of PDAC cells without the induction of apoptosis. The antiproliferative effect, caused by G2 cell cycle arrest, was strongly associated with increased expression of p21 and decreased expression of c-Myc and cyclin D1, and was induced only by combined treatment. Additionally, decreased proliferation could also be associated with the inhibition of the P13K/Akt and MAPK signaling pathways. Importantly, combination treatment reduced the colony-forming ability of PDAC cells, as compared to both compounds alone. Collectively, we showed that combined treatment with low concentrations of sorafenib and betulinic acid had the capacity to inhibit proliferation and abolish clonogenic activity in PDAC cell lines.
Allogeneic Mesenchymal Stem Cells and Biomaterials: The Perfect Match for Cardiac Repair?
Authors: Perez-Estenaga Inigo , Prosper Felipe , Pelacho Beatriz
Published: October 2018

Coronary heart disease is the leading cause of death worldwide with huge socio-economic consequences. Cell therapy, and particularly mesenchymal stem cells (MSC), are considered a promising option to treat this disorder, due to their robust trophic and immunomodulatory properties. However, limitations such as their low rate of engraftment and poor survival after administration into the heart have precluded their large-scale clinical use. Nevertheless, the combination of MSC with polymer-made scaffolds or hydrogels has proven to enhance their retention and, therefore, their efficacy. Additionally, their allogeneic use could permit the creation of ready-to-use cell patches able to improve their feasibility and promote their application in clinical settings. In this review, the experimental and clinical results derived from the use of MSC in cardiac pathology, as well as advances in the bioengineering field to improve the potential of therapeutic cells, are extensively discussed. Additionally, the current understanding of the heart response to the allogeneic MSC transplants is addressed.
Genome-Wide Association Study Reveals Novel Genomic Regions Associated with 10 Grain Minerals in Synthetic Hexaploid Wheat
Authors: Bhatta Madhav , Baenziger P. Stephen , Waters Brian M. , Poudel Rachana , Belamkar Vikas , Poland Jesse , Morgounov Alexey
Published: October 2018

Synthetic hexaploid wheat (SHW; Triticum durum L. × Aegilops tauschii Coss.) is a means of introducing novel genes/genomic regions into bread wheat (T. aestivum L.) and a potential genetic resource for improving grain mineral concentrations. We quantified 10 grain minerals (Ca, Cd, Cu, Co, Fe, Li, Mg, Mn, Ni, and Zn) using an inductively coupled mass spectrometer in 123 SHWs for a genome-wide association study (GWAS). A GWAS with 35,648 single nucleotide polymorphism (SNP) markers identified 92 marker-trait associations (MTAs), of which 60 were novel and 40 were within genes, and the genes underlying 20 MTAs had annotations suggesting a potential role in grain mineral concentration. Twenty-four MTAs on the D-genome were novel and showed the potential of Ae. tauschii for improving grain mineral concentrations such as Ca, Co, Cu, Li, Mg, Mn, and Ni. Interestingly, the large number of novel MTAs (36) identified on the AB genome of these SHWs indicated that there is a lot of variation yet to be explored and to be used in the A and B genome along with the D-genome. Regression analysis identified a positive correlation between a cumulative number of favorable alleles at MTA loci in a genotype and grain mineral concentration. Additionally, we identified multi-traits and stable MTAs and recommended 13 top 10% SHWs with a higher concentration of beneficial grain minerals (Cu, Fe, Mg, Mn, Ni, and Zn), a large number of favorable alleles compared to low ranking genotypes and checks that could be utilized in the breeding program for the genetic biofortification. This study will further enhance our understanding of the genetic architecture of grain minerals in wheat and related cereals.
Endometrial Stem Cell Markers: Current Concepts and Unresolved Questions
Authors: Tempest Nicola , Maclean Alison , Hapangama Dharani K.
Published: October 2018

The human endometrium is a highly regenerative organ undergoing over 400 cycles of shedding and regeneration over a woman’s lifetime. Menstrual shedding and the subsequent repair of the functional layer of the endometrium is a process unique to humans and higher-order primates. This massive regenerative capacity is thought to have a stem cell basis, with human endometrial stromal stem cells having already been extensively studied. Studies on endometrial epithelial stem cells are sparse, and the current belief is that the endometrial epithelial stem cells reside in the terminal ends of the basalis glands at the endometrial/myometrial interface. Since almost all endometrial pathologies are thought to originate from aberrations in stem cells that regularly regenerate the functionalis layer, expansion of our current understanding of stem cells is necessary in order for curative treatment strategies to be developed. This review critically appraises the postulated markers in order to identify endometrial stem cells. It also examines the current evidence supporting the existence of epithelial stem cells in the human endometrium that are likely to be involved both in glandular regeneration and in the pathogenesis of endometrial proliferative diseases such as endometriosis and endometrial cancer.
γ-Tubulin–γ-Tubulin Interactions as the Basis for the Formation of a Meshwork
Authors: Rosselló Catalina Ana , Lindström Lisa , Eklund Greta , Corvaisier Matthieu , Kristensson Maria Alvarado
Published: October 2018

In cytoplasm, protein γ-tubulin joins with various γ-tubulin complex proteins (GCPs) to form a heterotetramer γ-tubulin small complex (γ-TuSC) that can grow into a ring-shaped structure called the γ-tubulin ring complex (γ-TuRC). Both γ-TuSC and γ-TuRC are required for microtubule nucleation. Recent knowledge on γ-tubulin with regard to its cellular functions beyond participation in its creation of microtubules suggests that this protein forms a cellular meshwork. The present review summarizes the recognized functions of γ-tubulin and aims to unite the current views on this protein.
Genome-Wide Characterization of the sHsp Gene Family in Salix suchowensis Reveals Its Functions under Different Abiotic Stresses
Authors: Li Jianbo , Zhang Jin , Jia Huixia , Yue Zhiqiang , Lu Mengzhu , Xin Xuebing , Hu Jianjun
Published: October 2018

Small heat shock proteins (sHsps) function mainly as molecular chaperones that play vital roles in response to diverse stresses, especially high temperature. However, little is known about the molecular characteristics and evolutionary history of the sHsp family in Salix suchowensis, an important bioenergy woody plant. In this study, 35 non-redundant sHsp genes were identified in S. suchowensis, and they were divided into four subfamilies (C, CP, PX, and MT) based on their phylogenetic relationships and predicted subcellular localization. Though the gene structure and conserved motif were relatively conserved, the sequences of the Hsp20 domain were diversified. Eight paralogous pairs were identified in the Ssu-sHsp family, in which five pairs were generated by tandem duplication events. Ka/Ks analysis indicated that Ssu-sHsps had undergone purifying selection. The expression profiles analysis showed Ssu-Hsps tissue-specific expression patterns, and they were induced by at least one abiotic stress. The expression correlation between two paralogous pairs (Ssu-sHsp22.2-CV/23.0-CV and 23.8-MT/25.6-MT) were less than 0.6, indicating that they were divergent during the evolution. Various cis-acting elements related to stress responses, hormone or development, were detected in the promoter of Ssu-sHsps. Furthermore, the co-expression network revealed the potential mechanism of Ssu-sHsps under stress tolerance and development. These results provide a foundation for further functional research on the Ssu-sHsp gene family in S. suchowensis.