bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling of Railway Stations Based on Queuing Networks
Authors: Bychkov Igor , Kazakov Alexander , Lempert Anna , Zharkov Maxim
Published: March 2021

Among the micro-logistic transport systems, railway stations should be highlighted, such as one of the most important transport infrastructure elements. The efficiency of the transport industry as a whole depends on the quality of their operation. Such systems have a complex multi-level structure, and the incoming traffic flow often has a stochastic character. It is known that the most effective approach to study the operation of such systems is mathematical modeling. Earlier, we proposed an approach to transport hub modeling using multiphase queuing systems with a batch Markovian arrival process (BMAP) as an incoming flow. In this paper, we develop the method by applying more complex models based on queuing networks that allow us to describe in detail the route of requests within an object with a non-linear hierarchical structure. This allows us to increase the adequacy of modeling and explore a new class of objects—freight railway stations and marshalling yards. Here we present mathematical models of two railway stations, one of which is a freight railway station located in Russia, and the other is a marshalling yard in the USA. The models have the form of queuing networks with BMAP flow. They are implemented as simulation software, and a numerical experiment is carried out. Based on the numerical results, some “bottlenecks” in the structure of the studied stations are determined. Moreover, the risk of switching to an irregular mode of operation is assessed. The proposed method is suitable for describing a wide range of cargo and passenger transport systems, including river ports, seaports, airports, and multimodal transport hubs. It allows a primary analysis of the hub operation and does not need large statistical information for parametric identification.
High-Temperature Synthesis of Metal–Matrix Composites (Ni-Ti)-TiB2
Authors: Promakhov Vladimir , Matveev Alexey , Schulz Nikita , Grigoriev Mikhail , Olisov Andrey , Vorozhtsov Alexander , Zhukov Alexander , Klimenko Victor
Published: March 2021

Currently, metal–matrix composite materials are some of the most promising types of materials, and they combine the advantages of a metal matrix and reinforcing particles/fibres. Within the framework of this article, the high-temperature synthesis of metal–matrix composite materials based on the (Ni-Ti)-TiB2 system was studied. The selected approaches make it possible to obtain composite materials of various compositions without contamination and with a high degree of energy efficiency during production processes. Combustion processes in the samples of a 63.5 wt.% NiB + 36.5 wt.% Ti mixture and the phase composition and structure of the synthesis products were researched. It has been established that the synthesis process in the samples proceeds via the spin combustion mechanism. It has been shown that self-propagating high-temperature synthesis (SHS) powder particles have a composite structure and consist of a Ni-Ti matrix and TiB2 reinforcement inclusions that are uniformly distributed inside it. The inclusion size lies in the range between 0.1 and 4 µm, and the average particle size is 0.57 µm. The obtained metal-matrix composite materials can be used in additive manufacturing technologies as ligatures for heat-resistant alloys, as well as for the synthesis of composites using traditional methods of powder metallurgy.
Modeling the Evolution of Construction Solutions in Residential Buildings’ Thermal Comfort
Authors: Reis Inês F. G. , Figueiredo António , Samagaio António
Published: March 2021

The evolution of the construction sector over the years has been marked by the replacement of high thermal inertia mass constructions by increasingly lighter solutions that are subject to greater thermal fluctuations and, consequently, thermal discomfort. To minimize these effects, energy demanding space conditioning technologies are implemented, contributing significantly to the sector’s share of global energy consumption. Enhanced constructive solutions involving phase-change materials have been developed to respond to the constructive thermal inertia loss, influencing buildings’ thermal and energy performance. This work aims to model the evolution of the construction over the last decades to understand to what extent constructive characteristics influence the occupants’ thermal comfort. For this purpose, typical and enhanced solutions representing distinct constructive periods were simulated using the EnergyPlus® software through its graphical interface DesignBuilder® and the thermal comfort of the different solutions was evaluated using the adaptive model for thermal comfort EN16798-1. The main results reveal that more restraining regulatory requirements are indeed mitigating thermal discomfort situations. However, overheating phenomena can rise, creating worrying consequences in the short-medium term. Thus, countries with mild climates such as Portugal, must pay special attention to these effects, which may be aggravated by climate change.
Concentrate Apple Juice Industry: Aroma and Pomace Valuation as Food Ingredients
Authors: Coelho Elisabete , Pinto Mariana , Bastos Rita , Cruz Marco , Nunes Cláudia , Rocha Sílvia M. , Coimbra Manuel A.
Published: March 2021

Apple concentrate juice industry generates a flavored coproduct (apple aroma) recovered in the evaporation process, which is poorly valuated due to the lack of chemical characterization and standardization. In this study, industry apple aroma was characterized, allowing for the identification of 37 compounds, the majority esters (20), alcohols (7), and aldehydes (4). The storage temperature did not affect its volatile composition. Five key compounds were selected and monitored for 10 months of storage, and also compared with other three productions of another season allowing for observation of the same Aroma Index. Apple pomace was also used to produce a hydrodistillate. Contrary to the apple aroma, apple pomace hydrodistillate was unpleasant, reflected in a different volatile composition. Although no additional aroma fraction could be obtained from this wet byproduct, when dried, apple pomace presented 15 volatile compounds with toasted, caramel, sweet, and green notes. The infusions prepared with the dried apple pomace exhibited 25 volatile compounds with a very pleasant (fruity, apple-like, citrus, and spicy notes) and intense aroma. The addition of sugar changed the volatile profile, providing a less intense flavor, with almond, caramel, and sweet notes. These results show that apple aroma and pomace are high-quality flavoring agents with high potential of valuation as food ingredients.
The Variation Characteristics and Influencing Factors of Base Flow of the Hexi Inland Rivers
Authors: Lei Yuxin , Jiang Xiaohui , Geng Wenjie , Zhang Jinyan , Zhao Huan , Ren Liqing
Published: March 2021

The climate is becoming warmer and more humid in the inland area of northwestern China. In addition, human activities have changed the underlying surface of the river basin, and the instability of the runoff changes has intensified. As a component of river runoff, the base flow reflects the impacts of climate change and human activities. Therefore, it is necessary to carry out research on the change in the base flow and its influencing factors in the context of climate change and human activities. In this study, a base flow method suitable for the inland rivers in northwestern China was assessed, and the variation rules and influencing factors of the base flow were analyzed. The results reveal that since the 1980s, the base flow of the Hexi inland rivers has exhibited an increasing trend, and the growth rate has exhibited the following order: western > central > eastern. The Base Flow Index (the proportion of the base flow to the total runoff in a period) values are in the range of 0.45–0.65. Overall, the change in the base flow of the Hexi inland rivers is the result of the coupling of climate factors and land-use change. The influence of land-use change on the base flow of the Hexi inland rivers gradually weakens from east to west, except for the Xiying River, while the influence of climate change gradually increases. The contribution rates of land-use change to the base flow in the eastern, central, and western regions were 75%, 55%, and 27%. Temperature and precipitation are the main climate factors affecting the change in the base flow in the western and central regions, respectively.
Health Effects and Exposure Assessment to Bioaerosols in Indoor and Outdoor Environments
Authors: Brągoszewska Ewa
Published: March 2021

The Atmosphere Special Issue entitled “Health Effects and Exposure Assessment to Bioaerosols in Indoor and Outdoor Environments” comprises five original papers [...]
An Efficient Chicken Search Optimization Algorithm for the Optimal Design of Fuzzy Controllers
Authors: Amador-Angulo Leticia , Castillo Oscar , Peraza Cinthia , Ochoa Patricia
Published: March 2021

A proposed architecture to design the optimal parameters of Membership Functions (MFs) of Type-1 Fuzzy Logic Systems (T1FLSs) using the Chicken Search Optimization (CSO) is applied to three Fuzzy Logic Controllers (FLCs) in this paper. Two types of MFs are considered in the study: triangular and trapezoidal ones. The performance and efficiency of the CSO algorithm are particularly good when perturbations are added during the execution in each control problem. Two benchmark control problems: Water Tank Controller and Inverted Pendulum Controller are considered for testing the proposed approach. Also, the optimal design of a fuzzy controller for trajectory tracking of an Autonomous Mobile Robot (AMR) is considered to test the CSO. The main goal is to highlight the efficiency of CSO algorithm in finding optimal fuzzy controllers of non-linear plants. Two types of perturbations are considered in each control problem. Results show that the CSO algorithm presents excellent results in the field of Fuzzy Logic Controllers. Two types of Fuzzy Inference Systems: Takagi-Sugeno and Mamdani FLSs, are implemented in this paper. The most important metrics usually applied in control are used in this paper, such as: Integral Time Absolute Error (ITAE), Integral Time Squared Error (ITSE), Integral Absolute Error (IAE), Integral Square Error (ISE), Mean Square Error (MSE), and Root Mean Square Error (RMSE).
Global Invasion Risk Assessment of Prosopis juliflora at Biome Level: Does Soil Matter?
Authors: Dakhil Mohammed A. , El-Keblawy Ali , El-Sheikh Mohamed A. , Halmy Marwa Waseem A. , Ksiksi Taoufik , Hassan Walaa A.
Published: March 2021

Prosopis juliflora is one of the most problematic invasive trees in tropical and subtropical regions. Understanding driving forces affecting the potential global distribution would help in managing its current and future spread. The role of climate on the global spatial distribution of P. juliflora has been well studied, but little is known about the role of soil and human impacts as potential drivers. Here, we used maximum entropy (MaxEnt) for species distribution modelling to understand the role of climate (C), soil (S) and human impacts (H), C+S, and C+S+H in controlling the potential invasion range of P. juliflora, and to project its global potential invasive risk. We defined the top threatened global biomes, as predicted by the best-selected model. The incorporation of the edaphic factors improved the model performance and enhanced the accuracy of the outcome. Our findings revealed that the potential invasion risk increases with increases in mean temperature of the driest quarter (Bio9), soil alkalinity and clay fractions. Arid and semi-arid lands are at the highest risk of invasion than other moist biomes.
Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production
Authors: Djisalov Mila , Knežić Teodora , Podunavac Ivana , Živojević Kristina , Radonic Vasa , Knežević Nikola Ž. , Bobrinetskiy Ivan , Gadjanski Ivana
Published: March 2021

Meat cultivation via cellular agriculture holds great promise as a method for future food production. In theory, it is an ideal way of meat production, humane to the animals and sustainable for the environment, while keeping the same taste and nutritional values as traditional meat and having additional benefits such as controlled fat content and absence of antibiotics and hormones used in the traditional meat industry. However, in practice, there is still a number of challenges, such as those associated with the upscale of cultured meat (CM). CM food safety monitoring is a necessary factor when envisioning both the regulatory compliance and consumer acceptance. To achieve this, a multidisciplinary approach is necessary. This includes extensive development of the sensitive and specific analytical devices i.e., sensors to enable reliable food safety monitoring throughout the whole future food supply chain. In addition, advanced monitoring options can help in the further optimization of the meat cultivation which may reduce the currently still high costs of production. This review presents an overview of the sensor monitoring options for the most relevant parameters of importance for meat cultivation. Examples of the various types of sensors that can potentially be used in CM production are provided and the options for their integration into bioreactors, as well as suggestions on further improvements and more advanced integration approaches. In favor of the multidisciplinary approach, we also include an overview of the bioreactor types, scaffolding options as well as imaging techniques relevant for CM research. Furthermore, we briefly present the current status of the CM research and related regulation, societal aspects and challenges to its upscaling and commercialization.
Urgent Need for Field Surveys of Coronaviruses in Southeast Asia to Understand the SARS-CoV-2 Phylogeny and Risk Assessment for Future Outbreaks
Authors: Seyran Murat , Hassan Sk. Sarif , Uversky Vladimir N. , Pal Choudhury Pabitra , Uhal Bruce D. , Lundstrom Kenneth , Attrish Diksha , Rezaei Nima , Aljabali Alaa A. A. , Ghosh Shinjini , Pizzol Damiano , Adadi Parise , El-Aziz Tarek Mohamed Abd , Kandimalla Ramesh , Tambuwala Murtaza M. , Lal Amos , Azad Gajendra Kumar , Sherchan Samendra P. , Baetas-da-Cruz Wagner , Palù Giorgio , Brufsky Adam M.
Published: March 2021

Phylogenetic analysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is focused on a single isolate of bat coronaviruses (bat CoVs) which does not adequately represent genetically related coronaviruses (CoVs) [...]
Identification of Hub Genes and Key Pathways Associated with Anti-VEGF Resistant Glioblastoma Using Gene Expression Data Analysis
Authors: Arya Kesavan R. , Bharath Chand Ramachandran P. , Abhinand Chandran S. , Nair Achuthsankar S. , Oommen Oommen V. , Sudhakaran Perumana R.
Published: March 2021

Anti-VEGF therapy is considered to be a useful therapeutic approach in many tumors, but the low efficacy and drug resistance limit its therapeutic potential and promote tumor growth through alternative mechanisms. We reanalyzed the gene expression data of xenografts of tumors of bevacizumab-resistant glioblastoma multiforme (GBM) patients, using bioinformatics tools, to understand the molecular mechanisms of this resistance. An analysis of the gene set data from three generations of xenografts, identified as 646, 873 and 1220, differentially expressed genes (DEGs) in the first, fourth and ninth generations, respectively, of the anti-VEGF-resistant GBM cells. Gene Ontology (GO) and pathway enrichment analyses demonstrated that the DEGs were significantly enriched in biological processes such as angiogenesis, cell proliferation, cell migration, and apoptosis. The protein–protein interaction network and module analysis revealed 21 hub genes, which were enriched in cancer pathways, the cell cycle, the HIF1 signaling pathway, and microRNAs in cancer. The VEGF pathway analysis revealed nine upregulated (IL6, EGFR, VEGFA, SRC, CXCL8, PTGS2, IDH1, APP, and SQSTM1) and five downregulated hub genes (POLR2H, RPS3, UBA52, CCNB1, and UBE2C) linked with several of the VEGF signaling pathway components. The survival analysis showed that three upregulated hub genes (CXCL8, VEGFA, and IDH1) were associated with poor survival. The results predict that these hub genes associated with the GBM resistance to bevacizumab may be potential therapeutic targets or can be biomarkers of the anti-VEGF resistance of GBM.
Lifetime Impact of Cow’s Milk on Overactivation of mTORC1: From Fetal to Childhood Overgrowth, Acne, Diabetes, Cancers, and Neurodegeneration
Authors: Melnik Bodo C.
Published: March 2021

The consumption of cow’s milk is a part of the basic nutritional habits of Western industrialized countries. Recent epidemiological studies associate the intake of cow’s milk with an increased risk of diseases, which are associated with overactivated mechanistic target of rapamycin complex 1 (mTORC1) signaling. This review presents current epidemiological and translational evidence linking milk consumption to the regulation of mTORC1, the master-switch for eukaryotic cell growth. Epidemiological studies confirm a correlation between cow’s milk consumption and birthweight, body mass index, onset of menarche, linear growth during childhood, acne vulgaris, type 2 diabetes mellitus, prostate cancer, breast cancer, hepatocellular carcinoma, diffuse large B-cell lymphoma, neurodegenerative diseases, and all-cause mortality. Thus, long-term persistent consumption of cow’s milk increases the risk of mTORC1-driven diseases of civilization. Milk is a highly conserved, lactation genome-controlled signaling system that functions as a maternal-neonatal relay for optimized species-specific activation of mTORC1, the nexus for regulation of eukaryotic cell growth, and control of autophagy. A deeper understanding of milk´s impact on mTORC1 signaling is of critical importance for the prevention of common diseases of civilization.
Rechargeable Concrete Battery
Authors: Zhang Emma Qingnan , Tang Luping
Published: March 2021

A rechargeable cement-based battery was developed, with an average energy density of 7 Wh/m2 (or 0.8 Wh/L) during six charge/discharge cycles. Iron (Fe) and zinc (Zn) were selected as anodes, and nickel-based (Ni) oxides as cathodes. The conductivity of cement-based electrolytes was modified by adding short carbon fibers (CF). The cement-based electrodes were produced by two methods: powder-mixing and metal-coating. Different combinations of cells were tested. The results showed that the best performance of the rechargeable battery was the Ni–Fe battery, produced by the metal-coating method.
Construction Logistics Centres Proposing Kitting Service: Organization Analysis and Cost Mapping
Authors: El Moussaoui Soufiane , Lafhaj Zoubeir , Leite Fernanda , Fléchard Julien , Linéatte Bruno
Published: March 2021

The construction industry represents an important part of the global economy but is known for many issues such as harmful emissions, low productivity, waste generation, poor ergonomics, and construction incidents and accidents. A logistical view has proved to be a solid basis for improving construction performance while construction logistics centres (CLC) are gaining interest and proposing more services such as Kitting. Since the CLC-kitting-based approach is a new phenomenon, it is still a relatively unexplored topic. Hence, this paper addresses how this configuration could be applied to construction projects and what are its related costs information. The research design is based on a single case study showing the CLC-kitting use by a French general contractor in a student housing project. Data has been collected through site visits, observations, project documents, and discussions conducted with construction managers and logistics planners. Data analysis identified realized effects from deploying CLC-kitting in construction projects. Therefore, recommendations are provided, at both strategic and operational levels, which can be leveraged in similar projects. Finally, an identification of constructions logistics costs shows their distribution along the supply chain and was evaluated at 14.7% over material purchasing price.
Seismic and Coastal Vulnerability Assessment Model for Buildings in Chile
Authors: Quiñones-Bustos Catalina , Bull Maria Teresa , Oyarzo-Vera Claudio
Published: March 2021

This article proposes a vulnerability assessment model for evaluating buildings’ expected seismic performance, as well as their vulnerability to tsunamis. The objective of this assessment is to provide appropriate information for decision makers regarding the need of repairs and reinforcement of buildings or other mitigation measures that need to be applied in a territory. A procedure for assessing seismic vulnerability and another methodology for evaluating tsunami vulnerability faced by coastal structures is presented. Finally, a method that integrates both procedures is proposed, providing a combined index of vulnerability. The assessment model was applied to the central area of the city of Talcahuano, Chile, which was affected by the 2010 Maule earthquake and tsunami.
Critical Review of Public Policies for the Rehabilitation of Housing Stock: The Case of Barcelona
Authors: Uzqueda Angel , Garcia-Almirall Pilar , Cornadó Còssima , Vima-Grau Sara
Published: March 2021

This article reviews rehabilitation programs in Barcelona, focusing on aspects such as the intervention model, actual public investment, and results. Programs of this type are not exclusive to Barcelona; however, similar examples can be found in other European cities, especially where European Union (EU) funding is present. After analyzing these models, we reached a series of general conclusions that may be of interest. Thus, an eminently practical and reflective analysis is presented, aimed at technicians and those responsible for the design of intervention policies in urban regeneration. Rehabilitation programs are increasingly focused on vulnerable settings, and in this context, the participation and service function of public administrations are particularly relevant. The general model for rehabilitation subsidies rarely takes into account the specific characteristics of disadvantaged urban settings, as the article will show. After analyzing the different policies presented in this research, we identified two essential requirements: detailed knowledge of the affected neighborhood, and ongoing evaluation of the development of programs—beyond simple management indicators—so negative effects can be corrected in time such as gentrification, real estate speculation, and other by-products that the intervention itself can promote. This study confirms that not all public investments have the expected results.
Epithelial to Mesenchymal Transition Regulates Surface PD-L1 via CMTM6 and CMTM7 Induction in Breast Cancer
Authors: Xiao Malina , Hasmim Meriem , Lequeux Audrey , Moer Kris Van , Tan Tuan Zea , Gilles Christine , Hollier Brett G. , Thiery Jean Paul , Berchem Guy , Janji Bassam , Noman Muhammad Zaeem
Published: March 2021

CMTM6 is a critical regulator of cell surface expression of PD-L1 in tumor cells, but little is known about the transcriptional regulation of CMTM6. Here we report that the expression of CMTM6 positively correlates with the epithelial to mesenchymal transition (EMT) score in breast cancer cell lines and with the major EMT marker Vimentin in triple-negative breast cancers (TNBC). We showed that CMTM6 is concomitantly overexpressed with PD-L1 in breast mesenchymal compared with the epithelial cells. Driving a mesenchymal phenotype in SNAI1-inducible MCF-7 cells (MCF-7Mes cells) increased both PD-L1 and CMTM6. CMTM6 silencing in MCF-7Mes cells partially reduced cell surface expression of PD-L1, indicating that a proportion of the PD-L1 on the surface of MCF-7Mes cells depends on CMTM6. We also found a positive correlation between CMTM3 and CMTM7 expression with EMT score in breast cancer cells, and with Vimentin in TNBC patients. Dual knockdown of CMTM6 and CMTM7 significantly decreased PD-L1 surface expression in MCF-7Mes cells, indicating that both CMTM6 and CMTM7 regulate the expression of PD-L1. This study highlights the importance of CMTM6 and CMTM7 in EMT-induced PD-L1 and suggests that EMT, CMTM6 or CMTM7 modulators can be combined with anti-PD-L1 in patients with highly aggressive breast cancer.
ANO1 Expression Orchestrates p27Kip1/MCL1-Mediated Signaling in Head and Neck Squamous Cell Carcinoma
Authors: Filippou Artemis , Pehkonen Henna , Karhemo Piia-Riitta , Väänänen Juho , Nieminen Anni I. , Klefström Juha , Grénman Reidar , Mäkitie Antti A. , Joensuu Heikki , Monni Outi
Published: March 2021

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors that derive from the mucosal epithelium of the upper aerodigestive tract and present high mortality rate. Lack of efficient targeted-therapies and biomarkers towards patients’ stratification are caveats in the disease treatment. Anoctamin 1 (ANO1) gene is amplified in 30% of HNSCC cases. Evidence suggests involvement of ANO1 in proliferation, migration, and evasion of apoptosis; however, the exact mechanisms remain elusive. Aim of this study was to unravel the ANO1-dependent transcriptional programs and expand the existing knowledge of ANO1 contribution to oncogenesis and drug response in HNSCC. We cultured two HNSCC cell lines established from primary tumors harboring amplification and high expression of ANO1 in three-dimensional collagen. Differential expression analysis of ANO1-depleted HNSCC cells demonstrated downregulation of MCL1 and simultaneous upregulation of p27Kip1 expression. Suppressing ANO1 expression led to redistribution of p27Kip1 from the cytoplasm to the nucleus and associated with a cell cycle arrested phenotype. ANO1 silencing or pharmacological inhibition resulted in reduction of cell viability and ANO1 protein levels, as well as suppression of pro-survival BCL2 family proteins. Collectively, these data provide insights of ANO1 involvement in HNSCC carcinogenesis and support the rationale that ANO1 is an actionable drug target.
Evolution of Surgical Treatment of Colorectal Liver Metastases in the Real World: Single Center Experience in 1212 Cases
Authors: Ratti Francesca , Cipriani Federica , Fiorentini Guido , Burgio Valentina , Ronzoni Monica , Della Corte Angelo , Cascinu Stefano , De Cobelli Francesco , Aldrighetti Luca
Published: March 2021

Background: In recent years, the treatment of colorectal liver metastases (CRLM) has undergone significant evolution thanks to technical improvements as well as oncological advances, which have been the subject of targeted studies aimed at understanding the details of this heterogeneous disease. The purpose of this study is to put together pieces of this complex scenario by providing an overview of the evolution that has occurred in the context of a single center within a multidisciplinary management approach. Methods: Between 2005 and 2020, 1212 resections for CRLM were performed at the Hepatobiliary Surgery Division of San Raffaele Hospital, Milan. The series was divided into three historical periods, which were compared in terms of disease characteristics and short- and long-term outcomes: Period 1, 2005–2009 (293 cases); Period 2, 2010–2014 (353 cases); Period 3, 2015–2020 (566 cases). The trends for surgical technical complexity, oncological burden of the disease, use of the laparoscopic approach and use of techniques for hepatic hypertrophy were analyzed year by year. Uni- and multivariate analyses were performed to identify factors associated with inclusion to a laparoscopic approach and with long-term prognosis. Results: The number of resections performed over the years progressively increased, with an increase in the number of cases with a high Clinical Risk Score and a high profile of technical complexity. The proportion of cases performed laparoscopically increased, but less rapidly compared to other malignant tumors. The risk of postoperative morbidity and mortality was similar in the three analyzed periods. Long-term survival, stratified by Clinical Risk Score, improved in Period 3, while overall survival remained unchanged. Conclusion: The cultural background, the maturation of technical expertise and the consolidation of the multidisciplinary team have resulted in safe expansion of the possibility to offer a curative opportunity to patients, while continuously implementing into clinical practice evidence provided by the literature.
Hepatitis B Virus X Protein (HBx) Suppresses Transcription Factor EB (TFEB) Resulting in Stabilization of Integrin Beta 1 (ITGB1) in Hepatocellular Carcinoma Cells
Authors: Zhang Chunyan , Yang Huan , Pan Liwei , Zhao Guangfu , Zhang Ruofei , Zhang Tianci , Xiao Zhixiong , Tong Ying , Zhang Yi , Hu Richard , Pandol Stephen , Han Yuan-Ping
Published: March 2021

Hepatitis B virus (HBV) infection is a major etiological risk for the incidence of hepatocellular carcinoma (HCC), and HBV X protein (HBx) is essential for oncogenic transformation. It is not known that if HBx can sabotage the lysosomal system for transformation and tumorigenesis, or its mechanism if it does have an effect. Examining clinical data, we observed that the downregulation of lysosomal components and transcription factor EB (TFEB) was associated with a poor prognosis of HCC patients. In HCC cells, we found that expression of HBx suppressed TFEB, impaired biogenesis of autophagic-lysosome, and promoted cellular dissemination. HBx mediated downregulation of TFEB led to impairment of autophagic/lysosomal biogenesis and flux, and consequently, accumulation of integrin beta 1 (ITGB1) for motility of HCC cells. Conversely, TFEB, in a steady-state condition, through induction of lysosomal biogenesis restrained ITGB1 levels and limited mobility of HCC cells. Specifically, overexpression of TFEB upregulated and activated the cysteine proteases including cathepsin L (CTSL) to degrade ITGB1. Conversely, expression of cystatin A (CSTA) or cystatin B (CSTB), the cellular inhibitors of lysosomal cysteine proteinases, spared ITGB1 from degradation and promoted dissemination of HCC cells. Taken together, this study suggests a potential mechanism for HBV-mediated malignancy, showing that HBx mediated downregulation of TFEB leads to accumulation of ITGB1 for HCC cell migration.
Integrin α10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration
Authors: Masoumi Katarzyna Chmielarska , Huang Xiaoli , Sime Wondossen , Mirkov Anna , Munksgaard Thorén Matilda , Massoumi Ramin , Lundgren-Åkerlund Evy
Published: March 2021

Glioblastoma (GB) is the most common and the most aggressive form of brain tumor in adults, which currently lacks efficient treatment strategies. In this study, we investigated the therapeutic effect of function-blocking antibodies targeting integrin α10β1 on patient-derived-GB cell lines in vitro and in vivo. The in vitro studies demonstrated significant inhibiting effects of the integrin α10 antibodies on the adhesion, migration, proliferation, and sphere formation of GB cells. In a xenograft mouse model, the effect of the antibodies on tumor growth was investigated in luciferase-labeled and subcutaneously implanted GB cells. As demonstrated by in vivo imaging analysis and caliper measurements, the integrin α10-antibodies significantly suppressed GB tumor growth compared to control antibodies. Immunohistochemical analysis of the GB tumors showed lower expression of the proliferation marker Ki67 and an increased expression of cleaved caspase-3 after treatment with integrin α10 antibodies, further supporting a therapeutic effect. Our results suggest that function-blocking antibody targeting integrin α10β1 is a promising therapeutic strategy for the treatment of glioblastoma.
Comparative Photo-Electrochemical and Photocatalytic Studies with Nanosized TiO2 Photocatalysts towards Organic Pollutants Oxidation
Authors: Monllor-Satoca Damián , Bonete Pedro , Djellabi Ridha , Cerrato Giuseppina , Operti Lorenza , Gómez Roberto , Bianchi Claudia Letizia
Published: March 2021

The size of TiO2 can significantly affect both its photocatalytic and photo-electrochemical properties, thus altering the photooxidation of organic pollutants in air or water. In this work, we give an account of the photo-electrochemical and photocatalytic features of some nanosized TiO2 commercial powders towards a model reaction, the photooxidation of acetone. Cyclic voltammograms (CV) of TiO2 particulate electrodes under UV illumination experiments were carried out in either saturated O2 or N2 solutions for a direct correlation with the photocatalytic process. In addition, the effect of different reaction conditions on the photocatalytic efficiency under UV light in both aqueous and gaseous phases was also investigated. CV curves with the addition of acetone under UV light showed a negative shift of the photocurrent onset, confirming the efficient transfer of photoproduced reactive oxygen species (ROSs), e.g., hydroxyl radicals or holes to acetone molecules. The photocatalytic experiments showed that the two nano-sized samples exhibit the best photocatalytic performance. The different photoactivity of the larger-sized samples is probably attributed to their morphological differences, affecting both the amount and distribution of free ROSs involved in the photooxidation reaction. Finally, a direct correlation between the photocatalytic measurements in gas phase and the photo-electrochemical measurements in aqueous phase is given, thus evincing the important role of the substrate-surface interaction with similar acetone concentrations.
Adsorption of VOCs Is a Key Step in Plasma-Catalyst Coupling: The Case of Acetone onto TiO2 vs. CeO2
Authors: Wang Xianjie , Barakat Christelle , Jia Zixian , Romanias Manolis N. , Thévenet Frédéric , Rousseau Antoine
Published: March 2021

If a number of literature studies point at the positive role of coupling materials with non-thermal plasma, particularly for Volatile Organic Compounds (VOC) removal, most of them focus on the direct plasma-material interaction to understand the coupling. However, a key contribution relies in the VOC–material interaction. Therefore, this study focuses on the adsorption step of targeted VOCs to provide a new insight on plasma–material coupling. The adsorption of acetone, used as probe VOC, is explored on two widespread coupling materials: TiO2 and CeO2. First, their behaviors are compared regarding acetone uptake. This process is reactive and creates other organic species than acetone on both surfaces. Second, the metal oxide behaviors are compared regarding ozone uptake. Interestingly, under typical VOC treatment configuration, i.e., with organics on their surfaces, ozone uptake is driven by the adsorbed organics, not directly by the metal oxides anymore. Finally, the ozonation of both materials, preliminary exposed to acetone, is explored through the evolution of the adsorbed organics and the corresponding mineralization, i.e., CO and CO2 formation. It evidences that the reactive adsorption of VOCs plays a key role in making the surface organics ready for an efficient oxidation and mineralization under post-plasma exposure.
From CO2 to Value-Added Products: A Review about Carbon-Based Materials for Electro-Chemical CO2 Conversion
Authors: Ramírez-Valencia Lilian D. , Bailón-García Esther , Carrasco-Marín Francisco , Pérez-Cadenas Agustín F.
Published: March 2021

The global warming and the dangerous climate change arising from the massive emission of CO2 from the burning of fossil fuels have motivated the search for alternative clean and sustainable energy sources. However, the industrial development and population necessities make the decoupling of economic growth from fossil fuels unimaginable and, consequently, the capture and conversion of CO2 to fuels seems to be, nowadays, one of the most promising and attractive solutions in a world with high energy demand. In this respect, the electrochemical CO2 conversion using renewable electricity provides a promising solution. However, faradaic efficiency of common electro-catalysts is low, and therefore, the design of highly selective, energy-efficient, and cost-effective electrocatalysts is critical. Carbon-based materials present some advantages such as relatively low cost and renewability, excellent electrical conductivity, and tunable textural and chemical surface, which show them as competitive materials for the electro-reduction of CO2. In this review, an overview of the recent progress of carbon-based electro-catalysts in the conversion of CO2 to valuable products is presented, focusing on the role of the different carbon properties, which provides a useful understanding for the materials design progress in this field. Development opportunities and challenges in the field are also summarized.
The Nigral Coup in Parkinson’s Disease by α-Synuclein and Its Associated Rebels
Authors: Sian-Hulsmann Jeswinder , Riederer Peter
Published: March 2021

The risk of Parkinson’s disease increases with age. However, the etiology of the illness remains obscure. It appears highly likely that the neurodegenerative processes involve an array of elements that influence each other. In addition, genetic, endogenous, or exogenous toxins need to be considered as viable partners to the cellular degeneration. There is compelling evidence that indicate the key involvement of modified α-synuclein (Lewy bodies) at the very core of the pathogenesis of the disease. The accumulation of misfolded α-synuclein may be a consequence of some genetic defect or/and a failure of the protein clearance system. Importantly, α-synuclein pathology appears to be a common denominator for many cellular deleterious events such as oxidative stress, mitochondrial dysfunction, dopamine synaptic dysregulation, iron dyshomeostasis, and neuroinflammation. These factors probably employ a common apoptotic/or autophagic route in the final stages to execute cell death. The misfolded α-synuclein inclusions skillfully trigger or navigate these processes and thus amplify the dopamine neuron fatalities. Although the process of neuroinflammation may represent a secondary event, nevertheless, it executes a fundamental role in neurodegeneration. Some viral infections produce parkinsonism and exhibit similar characteristic neuropathological changes such as a modest brain dopamine deficit and α-synuclein pathology. Thus, viral infections may heighten the risk of developing PD. Alternatively, α-synuclein pathology may induce a dysfunctional immune system. Thus, sporadic Parkinson’s disease is caused by multifactorial trigger factors and metabolic disturbances, which need to be considered for the development of potential drugs in the disorder.
Tumor Necrosis Factor (TNF) Is Required for Spatial Learning and Memory in Male Mice under Physiological, but Not Immune-Challenged Conditions
Authors: Mygind Leda , Bergh Marianne Skov-Skov , Tejsi Vivien , Vaitheeswaran Ramanan , Lambertsen Kate L. , Finsen Bente , Metaxas Athanasios
Published: March 2021

Increasing evidence demonstrates that inflammatory cytokines—such as tumor necrosis factor (TNF)—are produced at low levels in the brain under physiological conditions and may be crucial for synaptic plasticity, neurogenesis, learning and memory. Here, we examined the effects of developmental TNF deletion on spatial learning and memory using 11–13-month-old TNF knockout (KO) and C57BL6/J wild-type (WT) mice. The animals were tested in the Barnes maze (BM) arena under baseline conditions and 48 h following an injection of the endotoxin lipopolysaccharide (LPS), which was administered at a dose of 0.5 mg/kg. Vehicle-treated KO mice were impaired compared to WT mice during the acquisition and memory-probing phases of the BM test. No behavioral differences were observed between WT and TNF-KO mice after LPS treatment. Moreover, there were no differences in the hippocampal content of glutamate and noradrenaline between groups. The effects of TNF deletion on spatial learning and memory were observed in male, but not female mice, which were not different compared to WT mice under baseline conditions. These results indicate that TNF is required for spatial learning and memory in male mice under physiological, non-inflammatory conditions, however not following the administration of LPS. Inflammatory signalling can thereby modulate spatial cognition in male subjects, highlighting the importance of sex- and probably age-stratified analysis when examining the role of TNF in the brain.
In-Situ Evaluation of the Protectivity of Coatings Applied to Metal Cultural Artefacts Using Non-Destructive Electrochemical Measurements
Authors: Mills Douglas J. , Schaefer Katarzyna , Wityk Tomasz
Published: March 2021

Electrochemical Noise Measurement (ENM) and DC electrolytic resistance measurement (ERM) can be used to assess the level of protectiveness provided by an organic coating (paint or varnish) to the underlying metal. These techniques also have applicability to the thinner, transparent type of coatings used to protect archaeological artefacts. Two studies are presented here demonstrating how ERM and ENM techniques can be applied in artefact preservation. The similarity of the techniques, both of which are a measure of resistance, means results can be considered to be analogous. The first study investigated the use of ERM to determine the protection levels provided by typical coatings in order to develop a database of coating type and application for objects, for specific environments. The second study used ENM to evaluate coatings which had been applied to historic artefacts recovered from shipwrecks in the Baltic Sea and displayed inside the museum or kept in the museum store area. The studies showed the usefulness of both techniques for determining the level of protection of a coating and how a better performing coating can be specified if a pre-existing coating on an artefact has been found to be unsuitable.
A Facile and Cost-Effective Method to Prepare a Robust Superhydrophobic RTV Silicone Coating
Authors: Liao Kaijin , Zhu Jiyuan
Published: March 2021

A facile and cost-effective layer-by-layer assembly method is reported for the fabrication of a superhydrophobic coating using room temperature vulcanized silicone rubber and silica particles of different diameters. The superhydrophobic coating shows a contact angle of 154.6° and a rolling angle of 9°. The coating exhibits excellent self-cleaning properties and corrosion resistance with good softness and mechanical durability. The sample maintains its good superhydrophobicity, even after a series of tests by knife scratching, cutting, and hammering. The fabrication method has great advantages in terms of efficiency, cost, simple procedure and conditions. Its wide application in different substrates and the use of environment-friendly materials are also beneficial to the large-scale production of the coating.
Mechanical Properties of Commercial Purity Aluminum Modified by Zirconium Micro-Additives
Authors: Mostafa Ahmad , Adaileh Wail , Awad Alaa , Kilani Adnan
Published: March 2021

The mechanical properties and the fractured surfaces of commercial purity aluminum modified by zirconium micro-additives were investigated by means of experimental examination. A commercial purity Al specimen was used as a reference material and seven Al-Zr alloys in the 0.02–0.14 wt.% Zr composition range (with 0.02 wt.% Zr step) were prepared by microalloying methods. Optical microscopy was used to examine the microstructures and to calculate the grain sizes of the prepared specimens. The phase assemblage diagrams were plotted and the relative amounts of solid phases were calculated at room temperature using FactSage thermochemical software and databases. Proof stress, strength coefficient and strain hardening exponent were measured from the stress-strain curves obtained from tensile experiments and Charpy impact energy was calculated for all specimens. The experiments showed that the grain size of commercial purity Al was reduced by adding any Zr concentration in the investigated composition range, which could be due to the nucleation of new grains at Al3Zr particle sites. Accordingly, the microhardness number, tensile properties and Charpy impact energy were improved, owing to the large grain-boundary areas resulted from the refining effect of Zr, which can limit the movement of dislocations in the refined samples. The basic fracture mode in all specimens was ductile, because Al has an FCC structure and remains ductile even at low temperatures. The ductile fractures took place in a transgranular manner as could be concluded from the fractured surface features, which include voids, ridges and cavitation.
Inflammation-Related Markers and Thyroid Function Measures in Pediatric Patients: Is the Grade of Obesity Relevant?
Authors: Țaranu Ioana , Lazea Cecilia , Creț Victoria , Răcătăianu Nicoleta , Iancu Mihaela , Bolboacă Sorana D.
Published: March 2021

We aimed to investigate the effect of weight status on inflammation-related markers and thyroid function tests in overweight and obese pediatric patients. Children and adolescents diagnosed between January 2017 and January 2019 with overweight or obesity were included in the study. Neutrophil-to-lymphocyte ratio (NLR), platelet-to lymphocyte ratio (PLR) and systemic immune-inflammation index (SII) were calculated for the groups defined according to Body Mass Index (BMI)-for-age z-score: overweight (≥1 BMI-for-age z-score), obese (≥2 BMI-for-age z-score) and severely obese (≥3 BMI-for-age z-score). Severely obese patients had significantly higher value of white blood cells (WBC) counts (median = 7.92) compared with overweight patients (7.37, p = 0.014). Absolute lymphocyte count was significantly associated with obesity degree in children (Spearman’s Rho coefficient ρ = 0.228. p = 0.035), whereas absolute polymorphonuclear neutrophils (PMNCs) count was significantly higher in severely obese adolescents than overweight adolescents (overweight: 4.04 vs. severely obese: 5.3 (p = 0.029)). In 8.19% of patients an elevated thyroid-stimulating hormone (TSH) level was found, and 3.36% of patients had a low level of free thyroxine with an elevated level of TSH. Total absolute WBC count may be a reliable inflammation-related marker in obese pediatric patients without metabolic syndrome, but needs to be validated in the context of all possible covariates. Subclinical and overt hypothyroidism may develop from an early age in overweight or obese patients.
Design and Initial Validation of a Questionnaire on Prospective Teachers’ Perceptions of the Landscape
Authors: Fernández Álvarez Rubén , Fernández José
Published: March 2021

This research focuses on the design, construction, and validation of a questionnaire that seeks to analyse the perception of the landscape amongst undergraduates studying for a Degree in Primary School Teaching at Salamanca University. The process has involved using both qualitative and quantitative techniques to test the content’s validity and the construct’s reliability and suitability through the participation of a panel of expert judges and a sample of 432 subjects. This has been followed by the introduction of an exploratory factor analysis (EFA) of the data provided by the cohort that has led to a study of the questionnaire’s core characteristics, a reduction in its size, and the validation of its pertinence.