bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between PNPLA3 rs738409 polymorphism and nonalcoholic fatty liver disease: a systematic review and meta-analysis
Authors: Salari Nader , Darvishi Niloufar , Mansouri Kamran , Ghasemi Hooman , Hosseinian-Far Melika , Darvishi Fateme , Mohammadi Masoud
Published: June 2021

Background

Non-alcoholic fatty liver disease (NAFLD) is a common disorder that is known to be the leading cause of chronic liver disease worldwide. This study aims to systematically review and meta-analyze the association between PNPLA3 rs738409 polymorphism and non-alcoholic fatty liver.

Methods

Following a systematic review and meta-analysis method, articles without any time limitation, were extracted from SID, MagIran, IranDoc, Scopus, Embase, Web of Science (WoS), PubMed and ScienceDirect international databases. Random effects model was used for analysis, and heterogeneity of studies was investigated considering the I2 index and using Comprehensive Meta-Analysis software.

Results

The odds ratio of CC genotype in patients with non-alcoholic fatty liver demonstrates the protective effect of CC genotype with the ratio of 0.52, whereas CG genotype presents an increasing effect of CG genotype with the ratio of 0.19, and GG genotype also showed an increasing effect of GG genotype with the ratio of 1.05. Moreover, CG + GG genotypes as a single group demostrated an odds rartio of 0.88.

Conclusion

This meta-analysis highlights that people with CC genotype has 52% lower chance of developing non-alcoholic fatty liver disease, and those with CG genotype had 19% higher risk of developing non-alcoholic fatty liver. Those with GG genotype were 105% more likely to develop non-alcoholic fatty liver than others. Moreover, those present in a population with CG + GG genotypes were 88% more likely to have non-alcoholic fatty liver disease.
A prenatal diagnosis case of partial duplication 21q21.1-q21.2 with normal phenotype maternally inherited
Authors: Jin Chunyan , Gu Zhiping , Jiang Xiaohan , Yu Pei , Xu Tianhui
Published: June 2021

Background

Down syndrome is characterized by trisomy 21 or partial duplication of chromosome 21. Extensive studies have focused on the identification of the Down Syndrome Critical Region (DSCR). We aim to provide evidence that duplication of 21q21.1-q21.2 should not be included in the DSCR and it has no clinical consequences on the phenotype.

Case presentation

Because serological screening was not performed at the appropriate gestational age, noninvasive prenatal testing (NIPT) analysis was performed for a pregnant woman with normal prenatal examinations at 22 weeks of gestation. The NIPT results revealed a 5.8 Mb maternally inherited duplication of 21q21.1-q21.2. To assess whether the fetus also carried this duplication, ultrasound-guided amniocentesis was conducted, and the result of chromosomal microarray analysis (CMA) with amniotic fluid showed a 6.7 Mb duplication of 21q21.1-q21.2 (ranging from position 18,981,715 to 25,707,009). This partial duplication of 21q21.1-q21.2 in the fetus was maternally inherited. After genetic counseling, the pregnant woman and her family decided to continue the pregnancy.

Conclusion

Our case clearly indicates that 21q21.1-q21.2 duplication is not included in the DSCR and most likely has no clinical consequences on phenotype.
Histone dynamics during DNA replication stress
Authors: Hsu Chia-Ling , Chong Shin Yen , Lin Chia-Yeh , Kao Cheng-Fu
Published: June 2021

Accurate and complete replication of the genome is essential not only for genome stability but also for cell viability. However, cells face constant threats to the replication process, such as spontaneous DNA modifications and DNA lesions from endogenous and external sources. Any obstacle that slows down replication forks or perturbs replication dynamics is generally considered to be a form of replication stress, and the past decade has seen numerous advances in our understanding of how cells respond to and resolve such challenges. Furthermore, recent studies have also uncovered links between defects in replication stress responses and genome instability or various diseases, such as cancer. Because replication stress takes place in the context of chromatin, histone dynamics play key roles in modulating fork progression and replication stress responses. Here, we summarize the current understanding of histone dynamics in replication stress, highlighting recent advances in the characterization of fork-protective mechanisms.
Genetic tools for the development of recombinant lactic acid bacteria
Authors: Wu Jiapeng , Xin Yongping , Kong Jian , Guo Tingting
Published: June 2021

Lactic acid bacteria (LAB) are a phylogenetically diverse group with the ability to convert soluble carbohydrates into lactic acid. Many LAB have a long history of safe use in fermented foods and are recognized as food-grade microorganisms. LAB are also natural inhabitants of the human intestinal tract and have beneficial effects on health. Considering these properties, LAB have potential applications as biotherapeutic vehicles to delivery cytokines, antigens and other medicinal molecules. In this review, we summarize the development of, and advances in, genome manipulation techniques for engineering LAB and the expected future development of such genetic tools. These methods are crucial for us to maximize the value of LAB. We also discuss applications of the genome-editing tools in enhancing probiotic characteristics and therapeutic functionalities of LAB.
Temporal trends and variation in out-of-pocket expenditures and patient cost sharing: evidence from a Chinese national survey 2011–2015
Authors: Qin Vicky Mengqi , Zhang Yuting , Chia Kee Seng , McPake Barbara , Zhao Yang , Hulse Emily S. G. , Legido-Quigley Helena , Lee John Tayu
Published: June 2021

Objectives

This study aims to examine: (1) temporal trends in the percentage of cost-sharing and amount of out-of-pocket expenditure (OOPE) from 2011 to 2015; (2) factors associated with cost-sharing and OOPE; and (3) the relationships between province-level economic development and cost-sharing and OOPE in China.

Setting

A total of 10,316 adults aged ≥45 years from China followed-up from 2011 to 2015 were included in the analysis. We measured two main outcome variables: (1) patient cost sharing, measured by the percentage of OOPE as total healthcare expenditure, and (2) absolute amount of OOPE.

Results

Based on self-reported data, we did not find substantial differences in the percentage of cost sharing, but a significant increase in the absolute amount of OOPE among the middle-aged and older Chinese between 2011 and 2015. The percentage of cost-sharing was considerably higher for outpatient than inpatient care, and the majority paid more than 80% of the total cost for prescription drugs. Provinces with higher GDP per capita tend to have lower cost-sharing and a higher OOPE than their counterparts, but the relationship for OOPE became insignificant after adjusting for individual factors.

Conclusion

Reducing out-of-pocket expenditure and patient cost sharing is required to improve financial protection from illness, especially for those with those with chronic conditions and reside in less developed regions in China. Ongoing monitoring of financial protection using data from various sources is warranted.
The impact of native Fallot anatomy on future therapeutic requirements and outcomes at follow-up
Authors: Ravaglioli Antonio , Ait-Ali Lamia , Federici Duccio , Salvadori Stefano , Pllumi Arketa , Pak Vitali , Marrone Chiara , Pizzuto Alessandra , Bonhoeffer Philipp , Festa Pierluigi
Published: June 2021

Background

In patients with repaired Fallot, subsequent surgical or interventional procedures and adverse cardiac events are frequent. We aimed to evaluate the impact of a simple pre-operative anatomic classification based on the size of the pulmonary valve (PV) annulus and branches on future therapeutic requirements and outcomes.

Method

This is a single-center retrospective analysis of patients operated for Fallot before the age of 2 years, from January 1990. Pre-operative anatomy, surgical and interventional procedures and adverse events were extrapolated from clinical records.

Results

Among the 312 patients, a description of the PV and pulmonary arteries (PAs) native anatomy was known in 239 patients (male:147, 61.5%), which were divided in the following 3 groups: group 1 (65 patients) with normal size of both PV and PAs; group 2 (108 patients) with PV hypoplasia but normal size PAs; group 3 (66 patients) with concomitant hypoplasia of the PV and PAs. During the 12.7 years (IQR 6.7–17) follow-up time, 23% of patients required at least one surgical or interventional procedure. At Kaplan–Meier analysis, there was a significant difference in requirement of future surgical or interventional procedures among the 3 groups (p < 0,001). At multivariate Cox regression analysis, hypoplasia of PV and PAs was an independent predictor of subsequent procedures (HR:3.1,CI:1.06–9.1,p = 0.03).

Conclusion

Native anatomy in Tetralogy of Fallot patients affects surgical strategy and follow-up. It would be therefore advisable to tailor patient’s counseling and follow-up according to native anatomy, rather than following a standardized protocol.
Dihydromyricetin ameliorates liver fibrosis via inhibition of hepatic stellate cells by inducing autophagy and natural killer cell-mediated killing effect
Authors: Zhou Xi , Yu Li , Zhou Min , Hou Pengfei , Yi Long , Mi Mantian
Published: June 2021

Background

This study investigated the mechanisms underlying the preventive effect of dihydromyricetin (DHM) against liver fibrosis involving hepatic stellate cells (HSCs) and hepatic natural killer (NK) cells.

Methods

A carbon tetrachloride (CCl4)-induced liver fibrosis model was established in C57BL/6 mice to study the antifibrotic effect of DHM based on serum biochemical parameters, histological and immunofluorescence stainings, and the expression of several fibrosis-related markers. Based on the immunoregulatory role of DHM, the effect of DHM on NK cell activation ex vivo was evaluated by flow cytometry. Then, we investigated whether DHM-induced autophagy was involved in HSCs inactivation using enzyme-linked immunosorbent assays, transmission electron microscopy, and western blot analysis. Thereafter, the role of DHM in NK cell-mediated killing was studied by in vitro coculture of NK cells and HSCs, with subsequent analysis by flow cytometry. Finally, the mechanism by which DHM regulates NK cells was studied by western blot analysis.

Results

DHM ameliorated liver fibrosis in C57BL/6 mice, as characterized by decreased serum alanine transaminase and aspartate transaminase levels, decreased expressions of collagen I alpha 1 (CoL-1α1), collagen I alpha 2 (CoL-1α2), tissue inhibitor of metalloproteinases 1 (TIMP-1), α-smooth muscle actin (α-SMA) and desmin, as well as increased expression of matrix metalloproteinase 1 (MMP1). Interestingly, HSCs activation was significantly inhibited by DHM in vivo and in vitro. As expected, DHM also upregulated autophagy-related indicators in liver from CCl4-treated mice. DHM also prevented TGF-β1-induced activation of HSCs in vitro by initiating autophagic flux. In contrast, the autophagy inhibitor 3-methyladenine markedly abolished the antifibrotic effect of DHM. Surprisingly, the frequency of activated intrahepatic NK cells was significantly elevated by DHM ex vivo. Furthermore, DHM enhanced NK cell-mediated killing of HSCs by increasing IFN-γ expression, which was abolished by an anti-IFN-γ neutralizing antibody. Mechanistically, DHM-induced IFN-γ expression was through AhR-NF-κB/STAT3 pathway in NK cells.

Conclusion

These results demonstrated that DHM can ameliorate the progression of liver fibrosis and inhibition of HSCs activation by inducing autophagy and enhancing NK cell-mediated killing through the AhR-NF-κB/STAT3-IFN-γ signaling pathway, providing new insights into the preventive role of DHM in liver fibrosis.
Adaptive planning based on single beam optimization in passive scattering carbon ion radiotherapy for patients with pancreatic cancer
Authors: Li Yang , Kubota Yoshiki , Okamoto Masahiko , Shiba Shintaro , Okazaki Shohei , Matsui Toshiaki , Tashiro Mutsumi , Nakano Takashi , Ohno Tatsuya
Published: June 2021

Background

Daily anatomical deviations may distort the dose distribution in carbon ion radiotherapy (CIRT), which may cause treatment failure. Therefore, this study aimed to perform re-planning to maintain the dose coverage in patients with pancreatic cancer with passive scattering CIRT.

Methods

Eight patients with pancreatic cancer and 95 daily computed tomography (CT) sets were examined. Two types of adaptive plans based on new range compensators (RCs) (AP-1) and initial RCs (AP-2) were generated. In AP-2, each beam was optimized by manually adjusting the range shifter thickness and spread-out Bragg peak size to make dose reduction by < 3% of the original plan. Doses of the original plan with bone matching (BM) and tumor matching (TM) were examined for comparison. We calculated the accumulated dose using the contour and intensity-based deformable image registration algorithm. The dosimetric differences in respect to the original plan were compared between methods.

Results

Using TM and BM, mean ± standard deviations of daily CTV V95 (%) difference from the original plan was − 5.1 ± 6.2 and − 8.8 ± 8.8, respectively, but 1.2 ± 3.4 in AP-1 and − 0.5 ± 2.1 in AP-2 (P < 0.001). AP-1 and AP-2 enabled to maintain a satisfactory accumulated dose in all patients. The dose difference was 1.2 ± 2.8, − 2,1 ± 1.7, − 7.1 ± 5.2, and − 16.5 ± 15.0 for AP-1, AP-2, TM, and BM, respectively. However, AP-2 caused a dose increase in the duodenum, especially in the left–right beam.

Conclusions

The possible dose deterioration should be considered when performing the BM, even TM. Re-planning based on single beam optimization in passive scattering CIRT seems an effective and safe method of ensuring the treatment robustness in pancreatic cancer. Further study is necessary to spare healthy tissues, especially the duodenum.
Zoology, chemical composition, pharmacology, quality control and future perspective of Musk (Moschus): a review
Authors: Liu Kai , Xie Long , Deng Mao , Zhang Xumin , Luo Jia , Li Xiaofang
Published: June 2021

Musk, the dried secretion from the musk sac gland which is located between the navel and genitals of mature male musk deer, is utilized as oriental medicine in east Asia. It has been utilized to treat conditions such as stroke, coma, neurasthenia, convulsions, and heart diseases in China since ancient times. This paper aims to provide a comprehensive overview of musk in zoology, chemical composition, pharmacology, clinical applications, and quality control according to the up-to-date literature. Studies found that musk mainly contains macrocyclic ketones, pyridine, steroids, fatty acids, amino acids, peptides, and proteins, whilst the main active ingredient is muscone. Modern pharmacological studies have proven that musk possesses potent anti-inflammatory effects, neuroprotective effects, anti-cancer effects, antioxidant effects, etc. Moreover, muscone, the main active ingredient, possesses anti-inflammatory, neuroprotective, antioxidant, and other pharmacological effects. In the quality control of musk, muscone is usually the main detection indicator, and the common analytical method is GC, and researchers have established novel and convenient methods such as HPLC-RI, RP-UPLC-ELSD, and Single-Sweep Polarography. In addition, quality evaluation methods based on steroids and the bioactivity of musk have been established. As for the identification of musk, due to various objective factors such as the availability of synthetic Muscone, it is not sufficient to rely on muscone alone as an identification index. To date, some novel technologies have also been introduced into the identification of musk, such as the electronic nose and DNA barcoding technology. In future research, more in vivo experiments and clinical studies are encouraged to fully explain the pharmacological effects and toxicity of musk, and more comprehensive methods are needed to evaluate and control the quality of musk.
Evaluation of whole-genome DNA methylation sequencing library preparation protocols
Authors: Morrison Jacob , Koeman Julie M. , Johnson Benjamin K. , Foy Kelly K. , Beddows Ian , Zhou Wanding , Chesla David W. , Rossell Larissa L. , Siegwald Emily J. , Adams Marie , Shen Hui
Published: June 2021

Background

With rapidly dropping sequencing cost, the popularity of whole-genome DNA methylation sequencing has been on the rise. Multiple library preparation protocols currently exist. We have performed 22 whole-genome DNA methylation sequencing experiments on snap frozen human samples, and extensively benchmarked common library preparation protocols for whole-genome DNA methylation sequencing, including three traditional bisulfite-based protocols and a new enzyme-based protocol. In addition, different input DNA quantities were compared for two kits compatible with a reduced starting quantity. In addition, we also present bioinformatic analysis pipelines for sequencing data from each of these library types.

Results

An assortment of metrics were collected for each kit, including raw read statistics, library quality and uniformity metrics, cytosine retention, and CpG beta value consistency between technical replicates. Overall, the NEBNext Enzymatic Methyl-seq and Swift Accel-NGS Methyl-Seq kits performed quantitatively better than the other two protocols. In addition, the NEB and Swift kits performed well at low-input amounts, validating their utility in applications where DNA is the limiting factor.

Results

The NEBNext Enzymatic Methyl-seq kit appeared to be the best option for whole-genome DNA methylation sequencing of high-quality DNA, closely followed by the Swift kit, which potentially works better for degraded samples. Further, a general bioinformatic pipeline is applicable across the four protocols, with the exception of extra trimming needed for the Swift Biosciences’s Accel-NGS Methyl-Seq protocol to remove the Adaptase sequence.
Variation on a theme: pigmentation variants and mutants of anemonefish
Authors: Klann Marleen , Mercader Manon , Carlu Lilian , Hayashi Kina , Reimer James Davis , Laudet Vincent
Published: June 2021

Pigmentation patterning systems are of great interest to understand how changes in developmental mechanisms can lead to a wide variety of patterns. These patterns are often conspicuous, but their origins remain elusive for many marine fish species. Dismantling a biological system allows a better understanding of the required components and the deciphering of how such complex systems are established and function. Valuable information can be obtained from detailed analyses and comparisons of pigmentation patterns of mutants and/or variants from normal patterns. Anemonefishes have been popular marine fish in aquaculture for many years, which has led to the isolation of several mutant lines, and in particular color alterations, that have become very popular in the pet trade. Additionally, scattered information about naturally occurring aberrant anemonefish is available on various websites and image platforms. In this review, the available information on anemonefish color pattern alterations has been gathered and compiled in order to characterize and compare different mutations. With the global picture of anemonefish mutants and variants emerging from this, such as presence or absence of certain phenotypes, information on the patterning system itself can be gained.
The role of lung ultrasound in COVID-19 disease
Authors:   European Society of Radiology (ESR), Clevert Dirk-André , Sidhu Paul S. , Lim Adrian , Ewertsen Caroline , Mitkov Vladimir , Piskunowicz Maciej , Ricci Paolo , Bargallo Núria , Brady Adrian P.
Published: June 2021

This statement summarises basic settings in lung ultrasonography and best practice recommendations for lung ultrasonography in COVID-19, representing the agreed consensus of experts from the Ultrasound Subcommittee of the European Society of Radiology (ESR). Standard lung settings and artefacts in lung ultrasonography are explained for education and training, equipment settings, documentation and self-protection.
Combining autologous bone marrow buffy coat and angioconductive bioceramic rod grafting with advanced core decompression improves short-term outcomes in early avascular necrosis of the femoral head: a prospective, randomized, comparative study
Authors: Li Qingtian , Liao Weihong , Fu Guangtao , Liao Junxing , Zhang Ruiying , Li Mengyuan , Yang Yuhui , Ma Yuanchen , Zheng Minghao , Zheng Qiujian
Published: June 2021

Background

Treatment of avascular necrosis of the femoral head (ANFH) in young patients remains a clinical challenge. A current controversy is whether hip-preserving surgery results in better outcomes. The adverse effects of hip-preserving surgery are associated with the fill material for the necrotic areas. This study aims to evaluate the early effects of autologous bone marrow buffy coat (BBC) and angioconductive bioceramic rod (ABR) grafting with advanced core decompression (ACD) on early ANFH.

Methods

Forty-four (57 hips) patients with early ANFH from 2015 to 2020 were recruited for this study. They were randomized into two groups: group A received ACD, BBC, and ABR grafting; group B received treatment of ACD with β-tricalcium phosphate (β-TCP) granules and ABR grafting. The outcomes were assessed using the Harris Hip Scores (HHS) and survival rate analysis. The follow-up endpoint was defined as conversion to total hip arthroplasty (THA).

Results

Forty patients (51 hips) were ultimately included in this study for analysis. Compared with group B, patients in group A had higher postoperative function score (P = 0.032) and postoperative Harris Hip Scores (HHS) (P = 0.041). Kaplan-Meier analysis showed a trend that the survivorship of the femoral head was higher in group A than in group B.

Conclusion

The short-term follow-up results showed that the autologous bone marrow buffy coat and angioconductive bioceramic rod grafting with advanced core decompression is effective in the treatment of early ANFH.

Trial registration

Chictr.org.cn, ChiCTR2000039595. Retrospectively registered on 11 February 2015.
The effects and potential applications of concentrated growth factor in dentin–pulp complex regeneration
Authors: Li Zixia , Liu Liu , Wang Liu , Song Dongzhe
Published: June 2021

The dentin–pulp complex is essential for the long-term integrity and viability of teeth but it is susceptible to damage caused by external factors. Because traditional approaches for preserving the dentin–pulp complex have various limitations, there is a need for novel methods for dentin–pulp complex reconstruction. The development of stem cell-based tissue engineering has given rise to the possibility of combining dental stem cells with a tissue-reparative microenvironment to promote dentin–pulp complex regeneration. Concentrated growth factor, a platelet concentrate, is a promising scaffold for the treatment of dentin–pulp complex disorders. Given its characteristics of autogenesis, convenience, usability, and biodegradability, concentrated growth factor has gained popularity in medical and dental fields for repairing bone defects and promoting soft-tissue healing. Numerous in vitro studies have demonstrated that concentrated growth factor can promote the proliferation and migration of dental stem cells. Here, we review the current state of knowledge on the effects of concentrated growth factor on stem cells and its potential applications in dentin–pulp complex regeneration.
Lawsonia intracellularis infected enterocytes lack sucrase-isomaltase which contributes to reduced pig digestive capacity
Authors: Helm Emma T. , Burrough Eric R. , Leite Fernando L. , Gabler Nicholas K.
Published: June 2021

Lawsonia intracellularisis endemic to swine herds worldwide, however much is still unknown regarding its impact on intestinal function. Thus, this study aimed to characterize the impact ofL. intracellularison digestive function, and how vaccination mitigates these impacts. Thirty-sixL. intracellularisnegative barrows were assigned to treatment groups (n =  12/trt): (1) nonvaccinated,L. intracellularisnegative (NC); (2) nonvaccinated,L intracellularischallenged (PC); and (3)L. intracellularischallenged, vaccinated (Enterisol®Ileitis, Boehringer Ingelheim) 7 weeks pre-challenge (VAC). On days post-inoculation (dpi) 0 PC and VAC pigs were inoculated withL. intracellularis. From dpi 19–21 fecal samples were collected for apparent total tract digestibility (ATTD) and at dpi 21, pigs were euthanized for sample collection. Post-inoculation, ADG was reduced in PC pigs compared with NC (41%,P <  0.001) and VAC (25%,P <  0.001) pigs. Ileal gross lesion severity was greater in PC pigs compared with NC (P =  0.003) and VAC (P =  0.018) pigs. Dry matter, organic matter, nitrogen, and energy ATTD were reduced in PC pigs compared with NC pigs (P ≤  0.001 for all). RNAscope in situ hybridization revealed abolition of sucrase-isomaltase transcript in the ileum of PC pigs compared with NC and VAC pigs (P <  0.01). Conversely, abundance of stem cell signaling markers Wnt3, Hes1, and p27Kip1were increased in PC pigs compared with NC pigs (P ≤  0.085). Taken together, these data demonstrate that reduced digestibility duringL. intracellularischallenge is partially driven by abolition of digestive machinery in lesioned tissue. Further, vaccination mitigated several of these effects, likely from lower bacterial burden and reduced disease severity.
Construction and characterization of a functional chimeric laccase from metagenomes suitable as a biocatalyst
Authors: Itoh Nobuya , Hayashi Yuya , Honda Serina , Yamamoto Yuna , Tanaka Daichi , Toda Hiroshi
Published: June 2021

Screening of gene-specific amplicons from metagenomes (S-GAM) is an efficient technique for the isolation of homologous genes from metagenomes. Using the S-GAM approach, we targeted multi-copper oxidase (MCO) genes including laccase and bilirubin oxidase (BOX) in soil and compost metagenomes, and successfully isolated novel MCO core regions. These core enzyme genes shared approximately 70% identity with that of the putative MCO from Micromonospora sp. MP36. According to the principle of S-GAM, the N- and C-terminal regions of the deduced products of the mature gene come from the known parent gene, which should be homologous and compatible with the target gene. We constructed two different MCO hybrid genes using Bacillus subtilis BOX and Micromonospora sp. MP36 MCO, to give Bs-mg-mco and Mic-mg-mco, respectively. The constructed chimeric MCO genes were fused with the maltose-binding protein (MBP) gene at the N-terminus for expression in Escherichia coli cells. We found that MBP-Mic-mg-MCO/Mic-mg-MCO possessed the characteristic properties of laccase, although MBP-Bs-mg-MCO had no activity. This novel laccase (Mic-mg-MCO) demonstrated unique substrate specificity, sufficient activity at neutral pH, and high thermal stability, which are suitable properties for its use as a laccase biocatalyst.
Intra-dialytic hypotension following the transition from continuous to intermittent renal replacement therapy
Authors: Beaubien-Souligny William , Yang Yifan , Burns Karen E. A. , Friedrich Jan O. , Meraz-Muñoz Alejandro , Clark Edward G. , Adhikari Neill K. , Bagshaw Sean M. , Wald Ron
Published: June 2021

Background

Transition from continuous renal replacement therapy (CRRT) to intermittent renal replacement therapy (IRRT) can be associated with intra-dialytic hypotension (IDH) although data to inform the definition of IDH, its incidence and clinical implications, are lacking. We aimed to describe the incidence and factors associated with IDH during the first IRRT session following transition from CRRT and its association with hospital mortality. This was a retrospective single-center cohort study in patients with acute kidney injury for whom at least one CRRT-to-IRRT transition occurred while in intensive care. We assessed associations between multiple candidate definitions of IDH and hospital mortality. We then evaluated the factors associated with IDH.

Results

We evaluated 231 CRRT-to-IRRT transitions in 213 critically ill patients with AKI. Hospital mortality was 43.7% (n = 93). We defined IDH during the first IRRT session as 1) discontinuation of IRRT for hemodynamic instability; 2) any initiation or increase in vasopressor/inotropic agents or 3) a nadir systolic blood pressure of < 90 mmHg. IDH during the first IRRT session occurred in 50.2% of CRRT-to-IRRT transitions and was independently associated with hospital mortality (adjusted odds ratio [OR]: 2.71; CI 1.51–4.84, p < 0.001). Clinical variables at the time of CRRT discontinuation associated with IDH included vasopressor use, higher cumulative fluid balance, and lower urine output.

Conclusions

IDH events during CRRT-to-IRRT transition occurred in nearly half of patients and were independently associated with hospital mortality. We identified several characteristics that anticipate the development of IDH following the initiation of IRRT.
A study of symmetric contractions with an application to generalized fractional differential equations
Authors: Hussain Aftab , Jarad Fahd , Karapinar Erdal
Published: June 2021

This article proposes four distinct kinds of symmetric contraction in the framework of complete F-metric spaces. We examine the condition to guarantee the existence and uniqueness of a fixed point for these contractions. As an application, we look for the solutions to fractional boundary value problems involving a generalized fractional derivative known as the fractional derivative with respect to another function.
Using “management mosaics” to mitigate the impacts from extreme wildfires
Authors: Guo Qinfeng
Published: June 2021

Human activities and global change have resulted in more severe and destructive megafires in forest ecosystems worldwide. Here, I introduce and discuss the concept of “management mosaics” and how to use it over both space and time to mitigate the growing impacts of extreme wildfires.
Altered ribosomal function and protein synthesis caused by tau
Authors: Evans Harrison Tudor , Taylor Deonne , Kneynsberg Andrew , Bodea Liviu-Gabriel , Götz Jürgen
Published: June 2021

The synthesis of new proteins is a fundamental aspect of cellular life and is required for many neurological processes, including the formation, updating and extinction of long-term memories. Protein synthesis is impaired in neurodegenerative diseases including tauopathies, in which pathology is caused by aberrant changes to the microtubule-associated protein tau. We recently showed that both global de novo protein synthesis and the synthesis of select ribosomal proteins (RPs) are decreased in mouse models of frontotemporal dementia (FTD) which express mutant forms of tau. However, a comprehensive analysis of the effect of FTD-mutant tau on ribosomes is lacking. Here we used polysome profiling, de novo protein labelling and mass spectrometry-based proteomics to examine how ribosomes are altered in models of FTD. We identified 10 RPs which were decreased in abundance in primary neurons taken from the K3 mouse model of FTD. We further demonstrate that expression of human tau (hTau) decreases both protein synthesis and biogenesis of the 60S ribosomal subunit, with these effects being exacerbated in the presence of FTD-associated tau mutations. Lastly, we demonstrate that expression of the amino-terminal projection domain of hTau is sufficient to reduce protein synthesis and ribosomal biogenesis. Together, these data reinforce a role for tau in impairing ribosomal function.
Developing a tool to assess technical skills in talented youth table tennis players—a multi-method approach combining professional and scientific literature and coaches’ perspectives
Authors: Faber Irene R. , Koopmann Till , Büsch Dirk , Schorer Jörg
Published: June 2021

Background

The assessment of technical skills as part of a multidimensional approach for talent identification and development in sports seems promising, especially in a technique-based sport like table tennis. However, current instruments mostly focus on a single element of technical skills, mainly use quantitative outcomes, and/or are not developed for talent purposes. Practice would benefit from a new instrument using a more ecologically valid approach. Thus, the purpose of this study was to identify the essential elements of technical skills in young table tennis players and to establish a first tool while using a multi-methods study design including an archive search for professional literature, a systematic search for scientific literature, as well as ten in-depth interviews with expert coaches.

Results

This approach taken ensured empirical findings to be combined with knowledge and experiences from the practical field and detailed explications by high-level expert coaches. Results for the literature searches yielded 23 professional and 21 scientific articles while data saturation was reached through all ten interviews. The triangulation process resulted in two general (i.e., individuality, interconnection between elements) and five specific (i.e., bat grip, ready position, footwork/body positioning, service, stroke) elements of technical skills in young table tennis players. In addition, criteria for both flawed and excellent executions were identified for each of the five specific elements. Finally, these results were used to create an observation sheet usable for an assessment during competition.

Conclusions

This study revealed the crucial elements of technical skills that should be taken into account when assessing sport-specific technical skills of youth table tennis players (8–12 years). Moreover, it provided concise descriptions of what is considered to be flawed or excellent executions of technical skills. Based on these findings, a first observation sheet, the Oldenburg observation sheet for Table Tennis Technique (O3T), was created to be used for the assessment of the current technical skill level within a competitive context at the early stage of a table tennis player’s career. Future research should focus on its measurement properties and its value within a multidimensional assessment for talent purposes.
Locomotive syndrome in hemodialysis patients and its association with quality of life—a cross-sectional study
Authors: Kitabayashi Kou , Yamamoto Suguru , Katano Yumi , Giustini Kayoko , Ei Isei , Ishii Yuji , Narita Ichiei
Published: June 2021

Background

Locomotive syndrome (LS) is defined as impairment of mobility function.

This study aimed to clarify LS and its association with quality of life in hemodialysis patients.

Methods

This is a cross-sectional study. The subjects were chronic kidney disease patients undergoing maintenance hemodialysis treatment. LS was assessed using two physical tests (two-step test, stand-up test) and one self-reported test (Geriatric Locomotive Function Scale-25). LS has two stages of severity; the beginning of the decline in mobility function is known as Locomo stage 1, and the progression of the decline of mobility function is known as Locomo stage 2. We used SF-36 to assess quality of life and examined their relationships with the Locomo stages. Chi-square test, Kruskal-Wallis test, Jonckheere-Terpstra test, and Mantel-Haenszel test were used for analysis. Multiple linear regression was used to model the cross-sectional association of Locomo stages with each component and summary score of SF-36.

Results

A total of 76 hemodialysis patients were included. The number of subjects with Locomo stage 1 and stage 2 were 19 (25%) and 53 (70%), respectively, while only four (5%) subjects did not have mobility dysfunction. Each component and summary score of the SF-36 for physical function, role emotional, physical component summary, and mental component summary were significantly associated with Locomo stages.

Conclusion

A high prevalence and severity of LS in hemodialysis patients was found, and the severity was associated with quality of life.
The toxic effect of zinc oxide nanoparticles on the terrestrial slug Lehmannia nyctelia (Gastropoda-Limacidae)
Authors: Mohammad Wafaa A. , Ali Safaa M. , Farhan Nasser , Said Shimaa Mohamed
Published: June 2021

Background

Chemicals have deleterious effect on the environment. The wide use of nanomaterials as products for plant protection, fertilizers, and also in water purification leads to the release of these materials to the environment. Terrestrial gastropods including snails and slugs have the ability to accumulate heavy metals in their bodies. The present study evaluates the toxic effect of zinc oxide nanoparticles on the terrestrial slug Lehmannia nyctelia. Zinc oxide nanoparticles (ZnO NPs) were prepared by thermal decomposition method. ZnO NPs are characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and ultraviolet-visible spectroscopy (UV). Slugs were treated with three different concentrations of ZnO NPs.

Results

A total of three animals died by the end of the experiment. Many histological alterations were detected after exposure to different concentration of ZnO NPs.

Conclusions

The obtained histological alterations prove the toxic effect of ZnO NPs on the animal under study.
Co-infection of Trichomonas vaginalis and HIV infection and its risk factors among prison inmates in Umuahia, Abia State, South Eastern Nigeria
Authors: Lawrence Queen Ozioma , Amadi Anthonia Nnenna Chuks , Okosa Chinenye , Ikpi Paulinus Obeten , Chukwuemeka Blessing Chigozie
Published: June 2021

Background

A study was conducted to determine the co-infection of Trichomonas vaginalis and HIV infection prevalence and its risk factors among prison inmates in Umuahia, Abia State, Nigeria. A total of 350 inmates (280 males and 70 females) participated in the study. Three hundred and fifty (350) blood and urine samples including 70 high vaginal swabs (from females) were screened using direct serological and wet mount methods respectively. Structured questionnaire were administered to obtain socio-demographic and behavioral risk factor data.

Results

An overall prevalence 43.4% was recorded for Trichomonas vaginalis single infection and 2.6% for HIV single infection. A total of 8(2.3%) prevalence for Trichomonas vaginalis and HIV co-infection were recorded in our study. Females recorded more infection (2.8%) than their male counterpart (2.1%). Age groups 21–30 and 31–40 had highest prevalence in male and age group 31–40 in females. This study also identified multiple sex partners and unprotected sex as the major risk factors that influence transmission of T. vaginalis and HIV infection although was insignificant.

Conclusions

Prevalence of T. vaginalis and HIV co-infection was minimal, however, should not be ignored. Adequate healthcare facility should be provided in the prison. Inmates should be tested and treated upon their release to reduce the spread of the infection to the general population.
Bacillus thuringiensis as microbial biopesticide: uses and application for sustainable agriculture
Authors: Kumar Pradeep , Kamle Madhu , Borah Rituraj , Mahato Dipendra Kumar , Sharma Bharti
Published: June 2021

Background

Bacillus thuringiensis (Bt) has been used in agriculture for a long time because of its insecticidal proteins which make it a valuable environment-friendly biopesticide. However, its use is not only limited to insecticidal properties. Current and previous studies indicate its potential as a biofertilizer for promoting plant growth, the development of transgenic plants, and others. It is the presence of δ-endotoxins, especially cry protein, which attributes the insecticidal property to the bacteria. Besides, there are some vegetative and secreted insecticidal proteins that exert their toxic activity towards specific species.

Main body of abstract

The present review briefly provides an overview of the Bt uses and application as a biocontrol agent against insect pest for sustainable agriculture. Historical development of Bt as biocontrol, classification of various cry proteins, their mechanisms of actions against different insect-pest, and incorporation of cry genes in the plant for developing transgenic Bt plants such as Bt cotton, potato, and maize. Applications of Bt as biofertilizer and the various bioformulations as biopesticide are also described.

Short conclusion

Uses of harmful pesticides and chemical cause various health issues and environmental problem; therefore, the Bt served as the best alternative to overcome the above issue. Also, we aim to explore the potential as plant growth-promoting potential and solubilization of minerals and the uses as a biofertilizer, keeping the high specificity and environmental safety of Bt. Its various formulations are commercially available and considered an efficient alternative to chemical pesticides.
Pyrimidine: a review on anticancer activity with key emphasis on SAR
Authors: Mahapatra Aastha , Prasad Tanya , Sharma Tripti
Published: June 2021

Background

Cancer is a global health challenge, it impacts the quality of life and its treatment is associated with several side effects. Resistance of the cancer cells to the existing drugs has led to search for novel anticancer agents. Pyrimidine, a privileged scaffold, is part of living organisms and plays vital role in various biological procedures as well as in cancer pathogenesis. Due to resemblance in structure with the nucleotide base pair of DNA and RNA, it is recognized as valuable compound in the treatment of cancer.

Main text

Many novel pyrimidine derivatives have been designed and developed for their anticancer activity in the last few years. The present review aims to focus on the structure activity relationship (SAR) of pyrimidine derivatives as anticancer agent from the last decade.

Conclusion

This review intends to assist in the development of more potent and efficacious anticancer drugs with pyrimidine scaffold.

Graphical abstract
Acute otitis media in an infant complicated with osteoarthritis of temporomandibular joint and sub-periosteal abscess of the clavicle
Authors: Cherrabi Kaoutar , Cherrabi Hind
Published: June 2021

Background

Otomastoiditis is a very frequent affection and a current complication of mal-treated benign ear infections in children. However, this a very rare case of the association of two rare complications of otomastoiditis in a newborn.

On the one hand, septic arthritis of the temporomandibular joint which is a very rare condition that is difficult to diagnose, and when unrecognized or not treated accordingly, it can resolve in serious infectious complication and or definitive injury to the temporomandibular joint.

On the other hand, osteomyelitis of the clavicle is also very rare, and only a few cases have been cited in the literature concerning infants.

Case presentation

This 46-day-old infant was brought to pediatric emergency consultation for 2 swelling inflammatory bulges, one in the right mastoid and pre-auricular regions, and another in the right basi-cervical area. The infant was hypertrophic febrile, hypotonic, and pale. He had preserved archaic reflexes. Besides, blood test showed an inflammatory syndrome, inflammatory anemia, and no other abnormalities.

Upon supplementary computed tomodensitometry exam, the diagnosis of a combination of septic arthritis of the right temporomandibular joint and sub-periosteal abscess of the ipsilateral clavicle in a context of hypotrophy and malnutrition was suspected. A pus sample was obtained for bacteriological evaluation, after which the infant had a course of intravenous associated antibiotics, along with nutritional assessment and management. Surgical drainage of both collections was performed. The 6-month follow-up was satisfactory, without clinical signs of functional impact on temporomandibular joint, or acromioclavicular joint.

Conclusion

This work stresses the necessity of thorough clinical examination of infants even in cases of benign ear infections, as well as the importance of adapted treatment and follow-up, which could allow early diagnosis, appropriate treatment, or even prevention of severe complications that can be associated with such benign conditions.
Novel Anaplastic Thyroid Cancer PDXs and Cell Lines: Expanding Preclinical Models of Genetic Diversity
Authors: Maniakas Anastasios , Henderson Ying C , Hei Hu , Peng Shaohua , Chen Yunyun , Jiang Yujie , Ji Shuangxi , Cardenas Maria , Chiu Yulun , Bell Diana , Williams Michelle D , Hofmann Marie-Claude , Scherer Steve E , Wheeler David A , Busaidy Naifa L , Dadu Ramona , Wang Jennifer R , Cabanillas Maria E , Zafereo Mark , Johnson Faye M , Lai Stephen Y
Published: June 2021

Context

Anaplastic thyroid cancer (ATC) is a rare, aggressive, and deadly disease. Robust preclinical thyroid cancer models are needed to adequately develop and study novel therapeutic agents. Patient-derived xenograft (PDX) models may resemble patient tumors by recapitulating key genetic alterations and gene expression patterns, making them excellent preclinical models for drug response evaluation.

Objective

We developed distinct ATC PDX models concurrently with cell lines and characterized them in vitro and in vivo.

Methods

Fresh thyroid tumor from patients with a preoperative diagnosis of ATC was surgically collected and divided for concurrent cell line and PDX model development. Cell lines were created by generating single cells through enzymatic digestion. PDX models were developed following direct subcutaneous implantation of fresh tumor on the flank of immune compromised/athymic mice.

Results

Six ATC PDX models and 4 cell lines were developed with distinct genetic profiles. Mutational characterization showed one BRAF/TP53/CDKN2A, one BRAF/CDKN2A, one BRAF/TP53, one TP53 only, one TERT-promoter/HRAS, and one TERT-promoter/KRAS/TP53/NF2/NFE2L2 mutated phenotype. Hematoxylin-eosin staining comparing the PDX models to the original patient surgical specimens show remarkable resemblance, while immunohistochemistry stains for important biomarkers were in full concordance (cytokeratin, TTF-1, PAX8, BRAF). Short tandem repeats DNA fingerprinting analysis of all PDX models and cell lines showed strong concordance with the original tumor. PDX successful establishment rate was 32%.

Conclusion

We have developed and characterized 6 novel ATC PDX models with 4 matching cell lines. Each PDX model harbors a distinct genetic profile, making them excellent tools for preclinical therapeutic trials.
Genetic Support of A Causal Relationship Between Iron Status and Type 2 Diabetes: A Mendelian Randomization Study
Authors: Wang Xinhui , Fang Xuexian , Zheng Wanru , Zhou Jiahui , Song Zijun , Xu Mingqing , Min Junxia , Wang Fudi
Published: June 2021

Context

Iron overload is a known risk factor for type 2 diabetes (T2D); however, iron overload and iron deficiency have both been associated with metabolic disorders in observational studies.

Objective

Using mendelian randomization (MR), we assessed how genetically predicted systemic iron status affected T2D risk.

Methods

A 2-sample MR analysis was used to obtain a causal estimate. We selected genetic variants strongly associated (P < 5 × 10−8) with 4 biomarkers of systemic iron status from a study involving 48 972 individuals performed by the Genetics of Iron Status consortium and applied these biomarkers to the T2D case-control study (74 124 cases and 824 006 controls) performed by the Diabetes Genetics Replication and Meta-analysis consortium. The simple median, weighted median, MR-Egger, MR analysis using mixture-model, weighted allele scores, and MR based on a Bayesian model averaging approaches were used for the sensitivity analysis.

Results

Genetically instrumented serum iron (odds ratio [OR]: 1.07; 95% CI, 1.02-1.12), ferritin (OR: 1.19; 95% CI, 1.08-1.32), and transferrin saturation (OR: 1.06; 95% CI, 1.02-1.09) were positively associated with T2D. In contrast, genetically instrumented transferrin, a marker of reduced iron status, was inversely associated with T2D (OR: 0.91; 95% CI, 0.87-0.96).

Conclusion

Genetic evidence supports a causal link between increased systemic iron status and increased T2D risk. Further studies involving various ethnic backgrounds based on individual-level data and studies regarding the underlying mechanism are warranted for reducing the risk of T2D.