bluuebunny/crossref_metadata_2025 huggingface dataset:
Degree of Early Estrogen Response Predict Survival after Endocrine Therapy in Primary and Metastatic ER-Positive Breast Cancer
Authors: Oshi Masanori , Tokumaru Yoshihisa , Angarita Fernando A. , Yan Li , Matsuyama Ryusei , Endo Itaru , Takabe Kazuaki
Published: November 2020

Endocrine therapy is the gold-standard treatment for ER-positive/HER2-negative breast cancer. Although its clear benefit, patient compliance is poor (50–80%) due to its long administration period and adverse effects. Therefore, a predictive biomarker that can predict whether endocrine therapy is truly beneficial may improve patient compliance. In this study, we use estrogen response early gene sets of gene set enrichment assay algorithm as the score. We hypothesize that the score could predict the response to endocrine therapy and survival of breast cancer patients. A total of 6549 breast cancer from multiple patient cohorts were analyzed. The score was highest in ER-positive/HER2-negative compared to the other subtypes. Earlier AJCC stage, as well as lower Nottingham pathological grade, were associated with a high score. Low score tumors enriched only allograft rejection gene set, and was significantly infiltrated with immune cells, and high cytolytic activity score. A low score was significantly associated with a worse response to endocrine therapy and worse survival in both primary and metastatic breast cancer patients. The hazard ratio was double that of ESR1 expression. In conclusion, the estrogen response early score predicts response to endocrine therapy and is associated with survival in primary and metastatic breast cancer.
Immunogenicity of Externally Activated Nanoparticles for Cancer Therapy
Authors: Sahin Onur , Meiyazhagan Ashokkumar , Ajayan Pulickel M. , Krishnan Sunil
Published: November 2020

Nanoparticles activated by external beams, such as ionizing radiation, laser light, or magnetic fields, have attracted significant research interest as a possible modality for treating solid tumors. From producing hyperthermic conditions to generating reactive oxygen species, a wide range of externally activated mechanisms have been explored for producing cytotoxicity within tumors with high spatiotemporal control. To further improve tumoricidal effects, recent trends in the literature have focused on stimulating the immune system through externally activated treatment strategies that result in immunogenic cell death. By releasing inflammatory compounds known to initiate an immune response, treatment methods can take advantage of immune system pathways for a durable and robust systemic anti-tumor response. In this review, we discuss recent advancements in radiosensitizing and hyperthermic nanoparticles that have been tuned for promoting immunogenic cell death. Our review covers both preclinical and clinical results, as well as an overview of possible future work.
Computed Tomography Radiomics for Residual Positron Emission Tomography-Computed Tomography Uptake in Lymph Nodes after Treatment
Authors: Kim Chu Hyun , Park Hyunjin , Lee Ho Yun , Ahn Joong Hyun , Lee Seung Hak , Sohn Insuk , Choi Joon Young , Kim Hong Kwan
Published: November 2020

Although a substantial decrease in 2-[fluorine-18]fluoro-2-deoxy-d-glucose (FDG) uptake on positron emission tomography-computed tomography (PET-CT) indicates a promising metabolic response to treatment, predicting the pathologic status of lymph nodes (LN) remains challenging. We investigated the potential of a CT radiomics approach to predict the pathologic complete response of LNs showing residual uptake after neoadjuvant concurrent chemoradiotherapy (NeoCCRT) in patients with non-small cell lung cancer (NSCLC). Two hundred and thirty-seven patients who underwent NeoCCRT for stage IIIa NSCLC were included. Two hundred fifty-two CT radiomics features were extracted from LNs showing remaining positive FDG uptake upon restaging PET-CT. A multivariable logistic regression analysis of radiomics features and clinicopathologic characteristics was used to develop a prediction model. Of the 237 patients, 135 patients (185 nodes) met our inclusion criteria. Eighty-seven LNs were proven to be malignant (47.0%, 87/185). Upon multivariable analysis, metastatic LNs were significantly prevalent in females and patients with adenocarcinoma (odds ratio (OR) = 2.02, 95% confidence interval (CI) = 0.88–4.62 and OR = 0.39, 95% CI = 0.19–0.77 each). Metastatic LNs also had a larger maximal 3D diameter and higher cluster tendency (OR = 9.92, 95% CI = 3.15–31.17 and OR = 2.36, 95% CI = 1.22–4.55 each). The predictive model for metastasis showed a discrimination performance with an area under the receiver operating characteristic curve of 0.728 (95% CI = 0.654–0.801, p value < 0.001). The radiomics approach allows for the noninvasive detection of metastases in LNs with residual FDG uptake after the treatment of NSCLC patients.
Health-Related Quality of Life in Patients with Philadelphia-Negative Myeloproliferative Neoplasms: A Nationwide Population-Based Survey in Denmark
Authors: Brochmann Nana , Flachs Esben Meulengracht , Christensen Anne Illemann , Bak Marie , Andersen Christen Lykkegaard , Juel Knud , Zwisler Ann-Dorthe
Published: November 2020

Previous studies have clarified that many patients with Philadelphia-negative myeloproliferative neoplasms (MPNs) have burdensome symptom profiles and accordingly impaired functioning and quality of life (QoL). In Denmark, all MPN patients are affiliated with public hospitals and because of a healthcare system financed by taxpayers these patients do not have any financial costs related to the hematological disease. Diagnoses are recorded for all patients in hospitals, and diagnosis codes are communicated to the National Patient Register (NPR). Owing to this, it was possible to contribute to the elucidation of Philadelphia-negative MPN patients’ health-related quality of life (HRQoL), by conducting a nationwide, population-based, cross-sectional HRQoL survey of these patients with cost-free access to the best available, suitable medical treatment. The survey contained validated questionnaires covering functioning, symptom burden, symptom profile, QoL, and additional questions on lifestyle. Information on comorbid diagnoses was obtained from the NPR. The participants’ HRQoL was compared to the general population. Moreover, differences in HRQoL across essential thrombocythemia, polycythemia vera, myelofibrosis, and unclassifiable MPN participants were investigated, adjusted for age, sex, comorbidity, and lifestyle. To the best of our knowledge this is the first survey of HRQoL in patients with unclassifiable MPN. A total of 2228 Philadelphia-negative MPN patients participated. The participants reported their HRQoL to be inferior to the general population, but the difference was minor. The differences in HRQoL across groups of participants with different MPN subtypes were subtle. Fatigue and sexual problems were prevalent and burdensome. Overall, participants reported a slightly healthier lifestyle compared to the general population.
Image-Guided Multisession Radiosurgery of Skull Base Meningiomas
Authors: Conti Alfredo , Pontoriero Antonio , Iatì Giuseppe , Cardali Salvatore M. , Brogna Anna , Friso Filippo , Rosetti Vittoria , Zoli Matteo , Parisi Silvana , Cacciola Alberto , Lillo Sara , Pergolizzi Stefano , Mazzatenta Diego
Published: November 2020

Background: The efficacy of single-session stereotactic radiosurgery (sSRS) for the treatment of intracranial meningioma is widely recognized. However, sSRS is not always feasible in cases of large tumors and those lying close to critically radiation-sensitive structures. When surgery is not recommended, multi-session stereotactic radiosurgery (mSRS) can be applied. Even so, the efficacy and best treatment schedule of mSRS are not yet established. The aim of this study is to validate the role of mSRS in the treatment of skull base meningiomas. Methods: A retrospective analysis of patients with skull base meningiomas treated with mSRS (two to five fractions) at the University of Messina, Italy, from 2008 to 2018, was conducted. Results: 156 patients met the inclusion criteria. The median follow-up period was 36.2 ± 29.3 months. Progression-free survival at 2-, 5-, and 10- years was 95%, 90%, and 80.8%, respectively. There were no new visual or motor deficits, nor cranial nerves impairments, excluding trigeminal neuralgia, which was reported by 5.7% of patients. One patient reported carotid occlusion and one developed brain edema. Conclusion: Multisession radiosurgery is an effective approach for skull base meningiomas. The long-term control is comparable to that obtained with conventionally-fractionated radiotherapy, while the toxicity rate is very limited.
RAC1B Regulation of TGFB1 Reveals an Unexpected Role of Autocrine TGFβ1 in the Suppression of Cell Motility
Authors: Ungefroren Hendrik , Otterbein Hannah , Wellner Ulrich F. , Keck Tobias , Lehnert Hendrik , Marquardt Jens-Uwe
Published: November 2020

Autocrine transforming growth factor (TGF)β has been implicated in epithelial-mesenchymal transition (EMT) and invasion of several cancers including pancreatic ductal adenocarcinoma (PDAC) as well as triple-negative breast cancer (TNBC). However, the precise mechanism and the upstream inducers or downstream effectors of endogenous TGFB1 remain poorly characterized. In both cancer types, the small GTPase RAC1B inhibits cell motility induced by recombinant human TGFβ1 via downregulation of the TGFβ type I receptor, ALK5, but whether RAC1B also impacts autocrine TGFβ signaling has not yet been studied. Intriguingly, RNA interference-mediated knockdown (RNAi-KD) or CRISPR/Cas-mediated knockout of RAC1B in TGFβ1-secreting PDAC-derived Panc1 cells resulted in a dramatic decrease in secreted bioactive TGFβ1 in the culture supernatants and TGFB1 mRNA expression, while the reverse was true for TNBC-derived MDA-MB-231 cells ectopically expressing RAC1B. Surprisingly, the antibody-mediated neutralization of secreted bioactive TGFβ or RNAi-KD of the endogenous TGFB1 gene, was associated with increased rather than decreased migratory activities of Panc1 and MDA-MB-231 cells, upregulation of the promigratory genes SNAI1, SNAI2 and RAC1, and downregulation of the invasion suppressor genes CDH1 (encoding E-cadherin) and SMAD3. Intriguingly, ectopic re-expression of SMAD3 was able to rescue Panc1 and MDA-MB-231 cells from the TGFB1 KD-induced rise in migratory activity. Together, these data suggest that RAC1B favors synthesis and secretion of autocrine TGFβ1 which in a SMAD3-dependent manner blocks EMT-associated gene expression and cell motility.
Assessing an Integral Treatment for Landfill Leachate Reverse Osmosis Concentrate
Authors: Tejera Javier , Hermosilla Daphne , Miranda Ruben , Gascó Antonio , Alonso Víctor , Negro Carlos , Blanco Ángeles
Published: November 2020

An integral treatment process for landfill leachate reverse osmosis concentrate (LLROC) is herein designed and assessed aiming to reduce organic matter content and conductivity, as well as to increase its biodegradability. The process consists of three steps. The first one is a coagulation/flocculation treatment, which best results were obtained using a dosage of 5 g L−1 of ferric chloride at an initial pH = 6 (removal of the 76% chemical oxygen demand (COD), 57% specific ultraviolet absorption (SUVA), and 92% color). The second step is a photo-Fenton process, which resulted in an enhanced biodegradability (i.e., the ratio between the biochemical oxygen demand (BOD5) and the COD increased from 0.06 to 0.4), and an extra 43% of the COD was removed at the best trialed reaction conditions of [H2O2]/COD = 1.06, pH = 4 and [H2O2]/[Fe]mol = 45. An ultra violet-A light emitting diode (UVA-LED) lamp was tested and compared to conventional high-pressure mercury vapor lamps, achieving a 16% power consumption reduction. Finally, an optimized 30 g L−1 lime treatment was implemented, which reduced conductivity by a 43%, and the contents of sulfate, total nitrogen, chloride, and metals by 90%. Overall, the integral treatment of LLROC achieved the removal of 99.9% color, 90% COD, 90% sulfate, 90% nitrogen, 86% Al, 77% Zn, 84% Mn, 99% Mg, and 98% Si; and significantly increased biodegradability up to BOD5/COD = 0.4.
Soot Combustion over Niobium-Doped Cryptomelane (K-OMS-2) Nanorods—Redox State of Manganese and the Lattice Strain Control the Catalysts Performance
Authors: Legutko Piotr , Gryboś Joanna , Fedyna Monika , Janas Janusz , Wach Anna , Szlachetko Jakub , Adamski Andrzej , Yu Xuehua , Zhao Zhen , Kotarba Andrzej , Sojka Zbigniew
Published: November 2020

A series of Nb-doped (0–23 wt%) cryptomelane catalyst (Nb-K-OMS-2) was synthesized and thoroughly characterized by XRD, TEM/EDX, XRF, XPS, XAS, UV-Vis, and Raman techniques corroborated by the work function measurements. The obtained catalysts were tested for soot oxidation (Printex U) in model tight and loose contact modes. It was shown that the catalytic properties of the Nb-K-OMS-2 are controlled by the amount of Nb dopant in a strongly non-monotonous way. The introduction of niobium gives rise to the strain in the cryptomelane lattice, accompanied by significant Mn+3/Mn+4 ratio variations and concomitant work function changes. The isotopic exchange experiments revealed that the catalytic activity of the Nb-OMS-2 catalysts in soot combustion proceeds via the pathways, where both the activated suprafacial 18O and the surface 16O2− species participate together in the reaction. The niobium doping level controls the non-monotonous changes of the catalyst work function and the lattice strain, and variations of these parameters correlate well with the observed deSoot activity. To our best knowledge, the role of the lattice strain of the cryptomelane catalysts was documented for the first time in this study.
Comparison of the Reactivity and Structures for the Neutral and Cationic Bis(imino)pyridyl Iron and Cobalt Species by DFT Calculations
Authors: Li Zilong , Ma Yanping , Sun Wen-Hua
Published: November 2020

Density Functional Theory (DFT) method was adopted to investigate and compare the reaction mechanisms of ethylene polymerization catalyzed by neutral, cationic bis(imino)pyridyl (PDI) iron and cobalt derivatives. The electronic structure and the oxidation states of the metal center and the PDI ligand were analyzed by taking spin states, natural bond orbital (NBO) charge distribution, etc. into consideration, revealing that the reactivity is closely related to the valence electron numbers instead of the charge numbers. The neutral Co(0) had the lowest reactivity as it possessed the most electrons. During the formation of the cationic Co(+)/Fe(+), one electron was mainly lost from PDI ligand rather than the metal center while the metal center maintained +II valence state through the process. Moreover, a special unsymmetrically bidentate N^N coordination manner was found to provide the deficient metal surroundings with 14e, which may initiate the reactivity of some unsymmetrical species with rich electrons. Finally, an anion [AlMe4]− participating process was proposed to explain the presence of the experimentally observed LCo(+)B(C2H4). A special intermediate, Co(+)B(C2H4) [AlMe4]− with Co in +I and absence of Co–C σ bond, was obtained. These calculation results may provide fundamental information for further understanding and designing the ethylene polymerization catalysts.
Potassium Acetate-Based Treatment for Thermally Co-Evaporated Perovskite Solar Cells
Authors: Li Jia , Wang Hao , Dewi Herlina Arianita , Mathews Nripan , Mhaisalkar Subodh , Bruno Annalisa
Published: November 2020

Thermal evaporation is a very successful and widely adopted coating technique for the deposition of organic and inorganic materials on rough and textured surfaces and over large areas. Indeed, this technique is extensively used in the semiconductor industry for the fabrication of organic light emitting diodes (OLEDs) and is commonly used in displays. In the last few years, thermal evaporated perovskite solar cells (PSCs) have also shown the potential to reach high power conversion efficiency (PCE) both on small and over large area devices. In this work, we present a detailed optimization of the potassium-based surface treatment used to improve the performances of our MAPbI3 PSCs fabricated using the thermal co-evaporation technique. Small area planar n-i-p PSCs with an active area of 0.16 cm2 achieved PCEs above 19% and the large area PSCs with an active area of 1 cm2 reached 18.1%. These un-encapsulated PSCs also proved an excellent long-term shelf stability maintaining 90% of their initial PCEs for over six months when stored at ambient temperature.
Electrical Conductivity and Optical Properties of Nanoscale Titanium Films on Sapphire for Localized Plasmon Resonance-Based Sensors
Authors: Korotkova Klavdiya , Bainov Dashi , Smirnov Serafim , Yunusov Igor , Zhidik Yury
Published: November 2020

The developing area of plasmonics has led to the possibility of creating a new type of high-speed, high-sensitivity optical sensor for biological environment analysis. The functional layer of such biosensors are nanoscale films of noble metals. In this work we suggest using a thin film of titanium as a functional layer. This paper presents the results of the research on electrical and optical characteristics of 5 to 80 nm thick titanium films deposited on sapphire substrates by magnetron sputtering. It is shown that surface plasmon resonance is consistently observed in the investigated titanium films and the theoretical grounds of surface plasmon resonance excitement is given. In structures with titanium films less than 15 nm thick, local plasmon resonance is observed along with surface plasmon resonance. Local plasmon resonance is more sensitive to the surface state of a thin film of titanium, which on the one hand increases the sensitivity of a biosensor, and on the other hand imposes restrictions on the parameters of nanoscale films.
Electrochemical Response of Clay/Polyelectrolyte Composite Barrier Coatings
Authors: Kim Sumin , Byeon Clare Chisu , Kim Sung Yeol
Published: November 2020

Composite materials made of polymer and clay are effective at blocking mass transport. In this study, the blocking efficacy of layer-by-layer (LbL) coatings of exfoliated montmorillonite (MMT) and polyethylenimine (PEI) was studied using cyclic voltammetry and a redox couple, indigo carmine (IC). The pH of the MMT solution was varied from 4 to 10 to prepare LbL coatings of different surface roughness on metal substrates. It was found that the coated electrode had a lower redox peak current value than without the coating, demonstrating the reduction of the mass transport of IC to the metal surface. The peak values decreased with decreasing the coating’s roughness and increasing the number of layers, indicating that the blocking capability can be controlled by changing the deposition conditions. Smooth LbL coatings deposited with MMT at pH 4 showed the highest blocking efficacy up to 97.5%. The IC adsorbed at the interface between the coating and the metal substrate was found to cause the peak current measured for the coated electrode. It was also confirmed that the same coating on the copper substrate reduced the corrosion of the copper during the electrochemical potential cycling.
An In-Situ Electroplating Fabricated Fabry-Perot Interferometric Sensor and Its Temperature Sensing Characteristics
Authors: Li Sijia , Li Yulong , Liu Xubo , Li Xuewen , Ding Tao , Ouyang Hua
Published: November 2020

In this study, in-situ electroplating method was used to fabricate a metal joint fixed extrinsic Fabry-Perot interferometric (EFPI) sensor. Specifically, optical fibers were firstly chemical plated with a very thin conductive nickel layer and then electroplated with nickel coating. After that, in-situ electroplating method was used to fix the metallized optical fibers and the capillary steel tube, the reflection spectra changes of the EFPI sensors during the in-situ electroplating process were recorded in real time, and the temperature sensing characteristics of the EFPI sensors were studied assisted by the temperature sensing system. Results show that: (i) optical fibers are well protected by the nickel layer; (ii) the reflection spectra of the EFPI sensors are clear and complete in the whole in-situ electroplating process, it is feasible to fabricate a EFPI sensor with the in-situ electroplating method; (iii) with the increases of temperature, the peak numbers of the reflection spectra of the EFPI sensors increase gradually; (iv) the EFPI sensors with different cavity length based on the in-situ electroplating method show excellent sensing characteristics, the temperature sensitivities reach up to about 700, 600, and 400 pm/°C from room temperature to 400 °C, respectively.
Influence of the Al/Ge Ratio on the Structure and Self-Organization of Anisometric Imogolite Nanotubes
Authors: Paineau Erwan , Launois Pascale
Published: November 2020

Synthetic imogolite-like nanotubes (INT) with well-defined diameters represent a considerable opportunity for the development of advanced functional materials. Recent progress has made it possible to increase their aspect ratio and unique self-organization properties were evidenced. We suggest that slight modification of the synthesis conditions may drastically affect the resulting liquid-crystalline properties. In this work, we investigate how the precursor’s [Al]/[Ge] molar ratio (R’) impacts the morphology and the colloidal properties of aluminogermanate INTs by combining a multi-scale characterization. While only double-walled nanotubes are found for R’ ≥ 1.8, the presence of single-walled nanotubes occurs when the ratio is lowered. Except for the lowest R’ ratio investigated (R’ = 0.66), all synthetic products present one-dimensional shapes with a high aspect ratio. Small-angle X-ray scattering experiments allow us to comprehensively investigate the colloidal properties of the final products. Our results reveal that a liquid-crystalline hexagonal columnar phase is detected down to R’ = 1.33 and that it turns into a nematic arrested phase for R’ = 0.90. These results could be useful for the development of novel stimuli-responsive nanocomposites based-on synthetic imogolite nanotubes.
Plant–Soil Interactions as Drivers of the Structure and Functions of Plant Communities
Authors: Faucon Michel-Pierre
Published: November 2020

Plant–soil interactions play an important role in the structure and function of plant communities and thus in the functioning of ecosystems [...]
Chinese Resident Preferences for African Elephant Conservation: Choice Experiment
Authors: Wang Shuokai , Cai Zhen , Hu Yuxuan , Cirella Giuseppe T. , Xie Yi
Published: November 2020

Despite passionate efforts to preserve African elephants worldwide, their numbers continue to decline. Some conservation programs have suspended operations because the funds provided by various governmental and non-governmental organizations (NGOs) cannot cover the enormous expenses of countering poaching, habitat destruction, and illegal ivory trading. This study investigates Chinese resident preferences for African elephant conservation using a choice experiment model. Results indicated that two-thirds of our 442 respondents with relatively higher education and income levels were willing to donate to conserve African elephants. Respondents were willing to donate RMB 1593.80 (USD 231.65) annually to African elephant conservation. Chinese residents were willing to donate the most to anti-poaching RMB 641.25 (USD 93.20), followed by enhancing habitat quality RMB 359.07 (USD 52.22), combating the illegal trade in ivory RMB 355.63 (USD 51.69), and alleviating human–elephant conflicts RMB 237.85 (USD 34.57). Our results suggest that accepting public donations could be an efficient way for NGOs to better preserve African elephants.
Convolutional Neural Network-Based Humerus Segmentation and Application to Bone Mineral Density Estimation from Chest X-ray Images of Critical Infants
Authors: Liu Yung-Chun , Lin Yung-Chieh , Tsai Pei-Yin , Iwata Osuke , Chuang Chuew-Chuen , Huang Yu-Han , Tsai Yi-Shan , Sun Yung-Nien
Published: November 2020

Measuring bone mineral density (BMD) is important for surveying osteopenia in premature infants. However, the clinical availability of dual-energy X-ray absorptiometry (DEXA) for standard BMD measurement is very limited, and it is not a practical technique for critically premature infants. Developing alternative approaches for DEXA might improve clinical care for bone health. This study aimed to measure the BMD of premature infants via routine chest X-rays in the intensive care unit. A convolutional neural network (CNN) for humeral segmentation and quantification of BMD with calibration phantoms (QRM-DEXA) and soft tissue correction were developed. There were 210 X-rays of premature infants evaluated by this system, with an average Dice similarity coefficient value of 97.81% for humeral segmentation. The estimated humerus BMDs (g/cm3; mean ± standard) were 0.32 ± 0.06, 0.37 ± 0.06, and 0.32 ± 0.09, respectively, for the upper, middle, and bottom parts of the left humerus for the enrolled infants. To our knowledge, this is the first pilot study to apply a CNN model to humerus segmentation and to measure BMD in preterm infants. These preliminary results may accelerate the progress of BMD research in critical medicine and assist with nutritional care in premature infants.
Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters
Authors: Hlapčić Iva , Belamarić Daniela , Bosnar Martina , Kifer Domagoj , Vukić Dugac Andrea , Rumora Lada
Published: November 2020

Interleukin (IL)-1α, IL-1β, IL-6, IL-8 and tumor necrosis factor (TNF)α contribute to inflammation in chronic obstructive pulmonary disease (COPD). We wanted to investigate their interrelations and association with disease severity, as well as to combine them with other inflammation-associated biomarkers and evaluate their predictive value and potential in identifying various patterns of systemic inflammation. One hundred and nine patients with stable COPD and 95 age- and sex-matched controls were enrolled in the study. Cytokines’ concentrations were determined in plasma samples by antibody-based multiplex immunosorbent assay kits. Investigated cytokines were increased in COPD patients but were not associated with disease or symptoms severity. IL-1β, IL-6 and TNFα showed the best discriminative values regarding ongoing inflammation in COPD. Inflammatory patterns were observed in COPD patients when cytokines, C-reactive protein (CRP), fibrinogen (Fbg), extracellular adenosine triphosphate (eATP), extracellular heat shock protein 70 (eHsp70) and clinical data were included in cluster analysis. IL-1β, eATP and eHsp70 combined correctly classified 91% of cases. Therefore, due to the heterogeneity of COPD, its assessment could be improved by combination of biomarkers. Models including IL-1β, eATP and eHsp70 might identify COPD patients, while IL-1β, IL-6 and TNFα combined with CRP, Fbg, eATP and eHsp70 might be informative regarding various COPD clinical subgroups.
Efficiency Evaluation on the Influence of Washing Methods for Biodiesel Produced from High Free Fatty Acid Waste Vegetable Oils through Selected Quality Parameters
Authors: Ortiz Lechuga Eugenia Guadalupe , Rodríguez Zúñiga Mauro , Arévalo Niño Katiushka
Published: November 2020

The increasing use of alternative energy sources has brought benefits like the recycling of waste vegetable oils (WVO) for biodiesel production; however, this practice presents challenges derived from a highly variable raw matter. Our research focused on the washing techniques usually employed by small to medium scale producers which may lack the infrastructure to perform high-grade purification and often relay on the employment of “artisanal” technology. We report biodiesel production from pure canola oil (C1) and a mix of soybean/canola oil (C2) as well as WVO from sample C2 with high free fatty acid content (2.7 FFA %) oil. We selected seven quality tests: pH determination, cloud, pour and flash point, acid number (AN), water/sediments and soap content considering the most commonly failed checkpoints in biodiesel production. From the use of four washing techniques, we determined that the sawdust filtration had the highest recovering yield with 89%. The majority of the washed biodiesel met the America Society of Testing Material (ASTM) standard limits for the tests selected. Biodiesel stability was tested from week 0 to week 6, determining a shelf life of three weeks in optimal conditions without the addition of antioxidants or specific storage conditions.
Sustainable Practices in Furniture Design: A Literature Study on Customization, Biomimicry, Competitiveness, and Product Communication
Authors: Bumgardner Matthew S. , Nicholls David L.
Published: November 2020

This research considers the ecosystem services associated with furniture, one of the leading types of secondary wood products manufactured internationally. We review and synthesize the literature surrounding the sustainable design, use, and disposition of wood furniture and related products in global markets. We consider emerging and innovative design strategies for wood (in biomimicry, for example) as well as topics that have been gaining traction in recent years (i.e., green supply chain management and eco/environmental labels and related market communications). An overarching theme is to consider how firm competitiveness can be influenced, or even enhanced, by green practices in design and associated communications with consumers. With a trend toward increasing customization in the secondary wood product marketplace, the role of design might be changing. However, design remains a critical product development function in modern markets, and designers are well-positioned to influence sustainable material utilization and improve furniture product use and lifespan.
A Disturbance Ecology Perspective on Silvicultural Site Preparation
Authors: Chaves Cardoso Jéssica , Burton Philip J. , Elkin Che M.
Published: November 2020

Silvicultural site preparation methods are used as planned disturbances for counteracting soil and vegetation constraints, as well as facilitating successful tree regeneration and growth. Understanding the possible effects of silvicultural site preparation on the ecosystem and evaluating site preparation as an ecological disturbance can help guide the selection and application of site preparation techniques for forest management goals. This review evaluates silvicultural site preparation techniques that are commonly used in boreal mixedwood ecosystems as agents of ecological disturbance by comparing the effects of each technique on the area disturbed and the degree of biomass modification, and then ordering them along a disturbance severity gradient. With a strong emphasis on the numerical estimation of the spatial footprint of different disturbances, broadcast burning typically has the highest disturbance severity, followed in order by broadcast herbicide use, mixing, plowing, disc trenching, mounding, scalping, and inverting. The evaluation of disturbance severity of various silvicultural site preparation techniques while using the proposed framework is feasible, in which quantitative assessments of area disturbed and biomass modification could be collected and assessed in most managed forests.
The Additions of Nitrogen and Sulfur Synergistically Decrease the Release of Carbon and Nitrogen from Litter in a Subtropical Forest
Authors: Tie Liehua , Fu Rao , Peñuelas Josep , Sardans Jordi , Zhang Shibin , Zhou Shixing , Hu Junxi , Huang Congde
Published: November 2020

Atmospheric nitrogen (N) and sulfur (S) deposition in subtropical forests has increased rapidly and the current level is very high, thus seriously affecting nutrient (e.g., N and phosphorus (P)) release from litter. However, the specific effects of S addition and its interaction with N on the release of carbon (C), N, and P from litter in subtropical evergreen broadleaved forests are unclear. Therefore, a two-year field experiment was performed using a litterbag method in a subtropical evergreen broadleaved forest in western China to examine the responses of litter decomposition and nutrient release to the control (CK), added N (+N), added S (+S), and added N and S (+NS) treatments. The results showed that the remaining litter mass, lignin, cellulose, C, N, P, and litter N/P ratio were higher, whereas the litter C/N ratio and soil pH were lower in the fertilization treatments than in CK. The annual decomposition coefficients (k-values) in the +N, +S, and +NS treatments were 0.384 ± 0.002, 0.378 ± 0.002, and 0.374 ± 0.001 year−1, respectively, which were significantly lower than the k-values in CK (0.452 ± 0.005 year−1, p < 0.05). The remaining mass, lignin, cellulose, C, and litter N/P ratio were higher, whereas the soil pH was lower in the +NS treatment than in the +N and +S. The interactive effects of N addition and S addition on the remaining litter lignin, cellulose, C, N, and P; the litter C/N, C/P, and N/P ratios; and the soil pH were significant (p < 0.05). In conclusion, the addition of N and S synergistically decreased the degradation of lignin and cellulose and the release of C and N and increased the litter N/P ratio, suggesting that external N and S inputs synergistically slowed the release of C and N from litter and exacerbated litter P limitation during decomposition in this forest.
Carbon Emissions from Oil Palm Induced Forest and Peatland Conversion in Sabah and Sarawak, Malaysia
Authors: Wan Mohd Jaafar Wan Shafrina , Said Nor Fitrah Syazwani , Abdul Maulud Khairul Nizam , Uning Royston , Latif Mohd Talib , Muhmad Kamarulzaman Aisyah Marliza , Mohan Midhun , Pradhan Biswajeet , Saad Siti Nor Maizah , Broadbent Eben North , Cardil Adrián , Silva Carlos Alberto , Takriff Mohd Sobri
Published: November 2020

The palm oil industry is one of the major producers of vegetable oil in the tropics. Palm oil is used extensively for the manufacture of a wide variety of products and its production is increasing by around 9% every year, prompted largely by the expanding biofuel markets. The rise in annual demand for biofuels and vegetable oil from importer countries has caused a dramatic increase in the conversion of forests and peatlands into oil palm plantations in Malaysia. This study assessed the area of forests and peatlands converted into oil palm plantations from 1990 to 2018 in the states of Sarawak and Sabah, Malaysia, and estimated the resulting carbon dioxide (CO2) emissions. To do so, we analyzed multitemporal 30-m resolution Landsat-5 and Landsat-8 images using a hybrid method that combined automatic image processing and manual analyses. We found that over the 28-year period, forest cover declined by 12.6% and 16.3%, and the peatland area declined by 20.5% and 19.1% in Sarawak and Sabah, respectively. In 2018, we found that these changes resulted in CO2 emissions of 0.01577 and 0.00086 Gt CO2-C yr−1, as compared to an annual forest CO2 uptake of 0.26464 and 0.15007 Gt CO2-C yr−1, in Sarawak and Sabah, respectively. Our assessment highlights that carbon impacts extend beyond lost standing stocks, and result in substantial direct emissions from the oil palm plantations themselves, with 2018 oil palm plantations in our study area emitting up to 4% of CO2 uptake by remaining forests. Limiting future climate change impacts requires enhanced economic incentives for land uses that neither convert standing forests nor result in substantial CO2 emissions.
Village Fengshui Forests as Forms of Cultural and Ecological Heritage: Interpretations and Conservation Policy Implications from Southern China
Authors: Chen Jianling , Lin Weiming , Zhang Yaoqi , Dai Yongwu , Chen Bixia
Published: November 2020

Using field survey data gathered from seven villages in Youxi County in southern China and a list of famous and ancient trees and groves, this study explored the cultural roots and ecological interpretations of village fengshui forests. The results show as follows: the species of fengshui trees selected by Chinese rural villagers bear a symbolic meaning, including health, longevity, flourishing population, fortune, and wealth. Fengshui belief, clan system, village rules and regulations, fengshui worship tradition, and customs have all played important roles in villagers’ planting and conservation of fengshui forests. It is suggested that the government should recognize the cultural and ecological value of fengshui forests and integrate the local rules and village regulations into the current policy frameworks to improve fengshui (culturally preserved) forests management with favourable polices such as subsidies and conservation strategy. China’s fengshui forests suggest the cultural importance of ecosystem services from forests, and the role of culture in forest conservation.
An Extended Car-Following Model Considering Generalized Preceding Vehicles in V2X Environment
Authors: Han Junyan , Zhang Jinglei , Wang Xiaoyuan , Liu Yaqi , Wang Quanzheng , Zhong Fusheng
Published: November 2020

Vehicle-to-everything (V2X) technology will significantly enhance the information perception ability of drivers and assist them in optimizing car-following behavior. Utilizing V2X technology, drivers could obtain motion state information of the front vehicle, non-neighboring front vehicle, and front vehicles in the adjacent lanes (these vehicles are collectively referred to as generalized preceding vehicles in this research). However, understanding of the impact exerted by the above information on car-following behavior and traffic flow is limited. In this paper, a car-following model considering the average velocity of generalized preceding vehicles (GPV) is proposed to explore the impact and then calibrated with the next generation simulation (NGSIM) data utilizing the genetic algorithm. The neutral stability condition of the model is derived via linear stability analysis. Numerical simulation on the starting, braking and disturbance propagation process is implemented to further study features of the established model and traffic flow stability. Research results suggest that the fitting accuracy of the GPV model is 40.497% higher than the full velocity difference (FVD) model. Good agreement between the theoretical analysis and the numerical simulation reveals that motion state information of GPV can stabilize traffic flow of following vehicles and thus alleviate traffic congestion.
Distinct Myocardial Transcriptomic Profiles of Cardiomyopathies Stratified by the Mutant Genes
Authors: Sielemann Katharina , Elbeck Zaher , Gärtner Anna , Brodehl Andreas , Stanasiuk Caroline , Fox Henrik , Paluszkiewicz Lech , Tiesmeier Jens , Wlost Stefan , Gummert Jan , Albaum Stefan P. , Sielemann Janik , Knöll Ralph , Milting Hendrik
Published: November 2020

Cardiovascular diseases are the number one cause of morbidity and mortality worldwide, but the underlying molecular mechanisms remain not well understood. Cardiomyopathies are primary diseases of the heart muscle and contribute to high rates of heart failure and sudden cardiac deaths. Here, we distinguished four different genetic cardiomyopathies based on gene expression signatures. In this study, RNA-Sequencing was used to identify gene expression signatures in myocardial tissue of cardiomyopathy patients in comparison to non-failing human hearts. Therefore, expression differences between patients with specific affected genes, namely LMNA (lamin A/C), RBM20 (RNA binding motif protein 20), TTN (titin) and PKP2 (plakophilin 2) were investigated. We identified genotype-specific differences in regulated pathways, Gene Ontology (GO) terms as well as gene groups like secreted or regulatory proteins and potential candidate drug targets revealing specific molecular pathomechanisms for the four subtypes of genetic cardiomyopathies. Some regulated pathways are common between patients with mutations in RBM20 and TTN as the splice factor RBM20 targets amongst other genes TTN, leading to a similar response on pathway level, even though many differentially expressed genes (DEGs) still differ between both sample types. The myocardium of patients with mutations in LMNA is widely associated with upregulated genes/pathways involved in immune response, whereas mutations in PKP2 lead to a downregulation of genes of the extracellular matrix. Our results contribute to further understanding of the underlying molecular pathomechanisms aiming for novel and better treatment of genetic cardiomyopathies.
PupStruct: Prediction of Pupylated Lysine Residues Using Structural Properties of Amino Acids
Authors: Singh Vineet , Sharma Alok , Dehzangi Abdollah , Tsunoda Tatushiko
Published: November 2020

Post-translational modification (PTM) is a critical biological reaction which adds to the diversification of the proteome. With numerous known modifications being studied, pupylation has gained focus in the scientific community due to its significant role in regulating biological processes. The traditional experimental practice to detect pupylation sites proved to be expensive and requires a lot of time and resources. Thus, there have been many computational predictors developed to challenge this issue. However, performance is still limited. In this study, we propose another computational method, named PupStruct, which uses the structural information of amino acids with a radial basis kernel function Support Vector Machine (SVM) to predict pupylated lysine residues. We compared PupStruct with three state-of-the-art predictors from the literature where PupStruct has validated a significant improvement in performance over them with statistical metrics such as sensitivity (0.9234), specificity (0.9359), accuracy (0.9296), precision (0.9349), and Mathew’s correlation coefficient (0.8616) on a benchmark dataset.
Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress
Authors: Gościńska Karolina , Shahmoradi Ghahe Somayeh , Domogała Sara , Topf Ulrike
Published: November 2020

Translation is a core process of cellular protein homeostasis and, thus, needs to be tightly regulated. The production of newly synthesized proteins adapts to the current needs of the cell, including the response to conditions of oxidative stress. Overall protein synthesis decreases upon oxidative stress. However, the selective production of proteins is initiated to help neutralize stress conditions. In contrast to higher eukaryotes, fungi require three translation elongation factors, eEF1, eEF2, and eEF3, for protein synthesis. eEF1 and eEF2 are evolutionarily conserved, but they alone are insufficient for the translation elongation process. eEF3 is encoded by two paralogous genes, YEF3 and HEF3. However, only YEF3 is essential in yeast, whereas the function of HEF3 remains unknown. To elucidate the cellular function of Hef3p, we used cells that were depleted of HEF3 and treated with H2O2 and analyzed the growth of yeast, global protein production, and protein levels. We found that HEF3 is necessary to withstand oxidative stress conditions, suggesting that Hef3p is involved in the selective production of proteins that are necessary for defense against reactive oxygen species.
Comprehensive Transcriptomic Analysis Identifies ST8SIA1 as a Survival-Related Sialyltransferase Gene in Breast Cancer
Authors: Kan Jung-Yu , Moi Sin-Hua , Hung Wen-Chun , Hou Ming-Feng , Chen Fang-Ming , Shih Shen-Liang , Shiau Jun-Ping , Li Chung-Liang , Chiang Chih-Po
Published: November 2020

Hypersialylation caused by the overexpression of sialyltransferases (STs) is a common feature in cancer that is associated with several characteristics of tumorigenesis. Thus, identifying cancer-associated STs is critical for cancer therapy. However, ST screening has been frequently conducted in cell line models. In this study, we conducted a comprehensive analysis of STs in the clinical database and identified the STs related with the survival of breast cancer patients. RNA sequencing (RNA-Seq) data of 496 patients were obtained from The Cancer Genome Atlas Breast Invasive Carcinoma (TCGA-BRCA). Of the eight mapped STs, ST3GAL5, and ST8SIA1 met the acceptable area under the curve (AUC) criteria for overall survival (OS). Using Kaplan–Meier methods, we determined that high expression of ST8SIA1 was associated with poor 10-year OS in all patients, triple-negative breast cancer (TNBC), and non-TNBC patients, and poor disease-free survival (DFS) rates particularly in TNBC. ST8SIA1 also had superior AUC values in terms of OS/DFS. High ST8SIA1 levels showed a higher risk for poor OS in different groups of patients and a higher risk for poor DFS particularly in TNBC. In summary, we conducted a comprehensive analysis of STs from the clinical database and identified ST8SIA1 as a crucial survival-related ST, which might be a potential therapeutic target for breast cancer and TNBC patients.
A Multilevel Procedure at Urban Scale to Assess the Vulnerability and the Exposure of Residential Masonry Buildings: The Case Study of Pordenone, Northeast Italy
Authors: Vettore Marco , Donà Marco , Carpanese Pietro , Follador Veronica , da Porto Francesca , Valluzzi Maria Rosa
Published: November 2020

More than the 60% of the Italian residential building stock had already been built by 1974, when seismic codes were enforced on a minimal part of the country. Unreinforced masonry buildings represent most of that share, but they are typical for each region, in terms of both materials and structural configurations. The definition of ‘regional’, i.e., more specific, vulnerability and exposure models are required to improve existing forecast models. The research presents a new geographic information system (GIS)-based multilevel procedure for earthquake disaster prevention planning at urban scale; it includes multicriteria analysis, such as architectural types, structural vulnerability analysis, microzonation studies, and socio-economic aspects. The procedure has been applied to the municipality of Pordenone (PN), a district town of the Friuli–Venezia–Giulia region, in Northeast Italy. To assess the urban seismic risk, more than 5000 masonry residential buildings were investigated and common types within sub-municipal areas and exposure data were collected. Simplified mechanical analysis provided a ‘regional’ vulnerability model through typological fragility curves. The integration of results into GIS tool permitted the definition of cross-mapping among vulnerability, damage scenarios (conditional and unconditional) and exposure (seismic losses, casualties, impact), with respect to various earthquake intensities expected in the town. These results are presented at different scales: from the single building, to submunicipal area and to the entire town.
Relationships between Perceived Stress, Depression and Alcohol Use Disorders in University Students during the COVID-19 Pandemic: A Socio-Economic Dimension
Authors: Gavurova Beata , Ivankova Viera , Rigelsky Martin
Published: November 2020

The objective of the study was to examine the effects of perceived stress on depression and subsequently to examine the effects of depression on alcohol use disorders. The data were obtained by an electronic questionnaire survey during the coronavirus disease 2019 (COVID-19) pandemic (n = 1523 Slovak university students). Descriptive, regression, and correlation analysis were used in the analytical processing, while the analyses included students’ scores in three diagnostic tools (Perceived Stress Scale (PSS), Patient Health Questionnaire for depression (PHQ 9), and Alcohol Use Disorders Identification Test (AUDIT)), as well as gender and income characteristics. The PSS identified an increased level of perceived stress in female students, while in contrast, the AUDIT showed an increased level of alcohol use disorders in male students. Differences in mental and behavioural disorders between the gender and income categories were significant in most of the analysed cases. In terms of gender-income characteristics, it was possible to confirm a significant positive effect of the PSS score on the PHQ 9 score, as well as a significant positive effect of the PHQ 9 score on the AUDIT score. As a result, efforts to reduce stress will be reflected in a reduction of depressive disorders as well as a reduction of excessive alcohol consumption among students.