bluuebunny/crossref_metadata_2025 huggingface dataset:
Pallister–Killian Syndrome versus Trisomy 12p—A Clinical Study of 5 New Cases and a Literature Review
Authors: Arghir Aurora , Popescu Roxana , Resmerita Irina , Budisteanu Magdalena , Butnariu Lacramioara Ionela , Gorduza Eusebiu Vlad , Gramescu Mihaela , Panzaru Monica Cristina , Papuc Sorina Mihaela , Sireteanu Adriana , Tutulan-Cunita Andreea , Rusu Cristina
Published: May 2021

Pallister–Killian syndrome (PKS) is a rare, sporadic disorder defined by a characteristic dysmorphic face, pigmentary skin anomalies, intellectual disability, hypotonia, and seizures caused by 12p tetrasomy due to an extra isochromosome 12p. We present three cases of PKS and two cases of trisomy 12p to illustrate and discuss features rarely cited in the literature, present certain particularities that not yet been cited, and analyze the differences between entities. Moreover, we present alternative methods of diagnosis that could be easily used in daily practice. Features not yet or rarely reported in PKS literature include marked excess of hair on the forehead and ears in the first months of life, a particular eye disorder (abnormal iris color with pointed pupil), connective tissue defects, repeated episodes of infection and autonomic dysfunction, endocrine malfunction as a possible cause of postnatal growth deficit, more complex sensory impairments, and mild early myoclonic jerks. After performing different combinations of tests, we conclude that MLPA (follow-up kit P230-B1) or array CGH using DNA extracted from a buccal swab is a reliable method of diagnosis in PKS and we recommend either one as a first intention diagnostic test. In cases without major defects associated (suspicion trisomy 12p), subtelomeric MLPA should be performed first.
Surveillance of Colorectal Cancer (CRC) in Cystic Fibrosis (CF) Patients
Authors: Ingravalle Fabio , Casella Giovanni , Ingravalle Adriana , Monti Claudio , De Salvatore Federica , Stillitano Domenico , Villanacci Vincenzo
Published: May 2021

Cystic Fibrosis (CF) is the commonest inherited genetic disorder in Caucasians due to a mutation in the gene CFTR (Cystic Fibrosis Transmembrane Conductance Regulator), and it should be considered as an Inherited Colorectal Cancer (CRC) Syndrome. In the United States, physicians of CF Foundation established the “Developing Innovative Gastroenterology Speciality Training Program” to increase the research on CF in gastrointestinal and hepatobiliary diseases. The risk to develop a CRC is 5–10 times higher in CF patients than in the general population and even greater in CF patients receiving immunosuppressive therapy due to organ transplantation (30-fold increased risk relative to the general population). Colonoscopy should be considered the best screening for CRC in CF patients. The screening colonoscopy should be started at the age of 40 in CF patients and, if negative, a new colonoscopy should be performed every 5 years and every 3 years if adenomas are detected. For transplanted CF patients, the screening colonoscopy could be started at the age of 35, in transplanted patients at the age of 30 and, if before, at the age of 30. CF transplanted patients, between the age of 35 and 55, must repeat colonoscopy every 3 years. Our review draws attention towards the clinically relevant development of CRC in CF patients, and it may pave the way for further screenings and studies.
The Impact of COVID-19 on Horticulture: Critical Issues and Opportunities Derived from an Unexpected Occurrence
Authors: Bulgari Roberta , Petrini Alice , Cocetta Giacomo , Nicoletto Carlo , Ertani Andrea , Sambo Paolo , Ferrante Antonio , Nicola Silvana
Published: May 2021

The COVID-19 pandemic is causing many victims worldwide and has generated a serious economic crisis. Substantial changes have occurred in the food and ornamental production chains. The aim of the present review has been to summarize some of the main effects that the pandemic is having on horticulture and on the new habits of people. Infections and quarantine measures have prevented the regular flow of certain goods and of connected services. Cases of shortages and/or surpluses, a lack of the availability of labor, and a reduction in demand for some food products and flowers have occurred. New food production approaches have emerged and a reconnection between farmers and consumers has been spreading, thereby facilitating product distribution. Moreover, during the forced isolation, people have had to face periods of stress. The benefits that can be derived from leisure activities related to flowers and ornamental plants, and from access to nature and urban green spaces are increasingly being recognized as relevant. The seriousness of the pandemic will inevitably lead to lasting changes. Therefore, the vulnerability of the pre-COVID-19 distribution chains should be considered and a new food production chain should be drawn up, to increase the resilience of such systems.
Foundational Statistical Principles in Medical Research: A Tutorial on Odds Ratios, Relative Risk, Absolute Risk, and Number Needed to Treat
Authors: Monaghan Thomas F. , Rahman Syed N. , Agudelo Christina W. , Wein Alan J. , Lazar Jason M. , Everaert Karel , Dmochowski Roger R.
Published: May 2021

Evidence-based medicine is predicated on the integration of best available research evidence with clinical expertise and patient values to inform care. In medical research, several distinct measures are commonly used to describe the associations between variables, and a sound understanding of these pervasive measures is foundational in the clinician’s ability to interpret, synthesize, and apply available evidence from the medical literature. Accordingly, this article aims to provide an educational tutorial/topic primer on some of the most ubiquitous measures of association and risk quantification in medical research, including odds ratios, relative risk, absolute risk, and number needed to treat, using several real-world examples from the medical literature.
Impact of Chemotherapy-Induced Peripheral Neuropathy on Quality of Life in Patients with Advanced Lung Cancer Receiving Platinum-Based Chemotherapy
Authors: Hung Hsing-Wei , Liu Chien-Ying , Chen Hsiu-Fang , Chang Chun-Chu , Chen Shu-Ching
Published: May 2021

Chemotherapy-induced peripheral neuropathy (CIPN) is a common adverse effect of neurotoxic anticancer drugs that may affect quality of life (QoL). Purpose: The purposes of this study were to: assess the levels of CIPN, anxiety, depression, CIPN–related QoL, and general QoL; and identify the factors related to CIPN–related QoL and general QoL in patients with advanced lung cancer (LC) receiving platinum-based chemotherapy. This cross-sectional study examined patients with advanced LC who received platinum-based chemotherapy from the thoracic oncology inpatient wards of a medical center in northern Taiwan. Structured questionnaires were used to measure patients’ CIPN (European Organization for Research and Treatment of Cancer quality of life questionnaire–chemotherapy–induced peripheral neuropathy 20), anxiety (Hospital Anxiety and Depression Scale Depression Scale [HADS]), depression (HADS), CIPN-related QoL (Functional Assessment of Cancer Therapy /Gynecologic Oncology Group-Neurotoxicity subscale [FACT/GOG–Ntx]), and general QoL (Functional Assessment of Cancer Therapy–General Input [FACT-G]). Of 93 patients with advanced LC, 53.8% reported CIPN–sensory impairment and 47.3% reported CIPN–motor impairment. The most common CIPN symptoms were difficulty getting or maintaining an erection (only for men > 65 years) and difficulty in climbing stairs or getting up out of a chair. Poor CIPN–related QoL (FACT/GOG–Ntx) was associated with more CIPN–sensory and more CIPN–motor impairment. Poor general QoL (FACT-G) was associated with a higher level of depression, a higher level of anxiety, and receipt of more chemotherapy cycles. More than half of LC patients report impairment related to CIPN, calling for holistic treatment to improve QoL.
Scuba Diving as a Form of Rehabilitation for People with Physical Disabilities
Authors: Henrykowska Gabriela , Soin Joanna , Siermontowski Piotr
Published: May 2021

(1) Background: The exploration of the potential therapeutic benefits of scuba diving for the mental and physical health of people with physical disabilities. (2) Methods: The research was conducted on a group of 240 people (men and women) with physical disabilities, using the survey designed by one of the authors. The subjective sense of physical and mental fitness was analyzed in retrospective and real terms. (3) Results: Significant increases in self-esteem, belief in our own abilities (self-confidence) and improvement in the ability to engage in social interactions were observed in the group of scuba divers with disabilities compared to individuals with disabilities not practicing diving. The respondents also declared an improvement in the efficiency of the respiratory system and stressed that a water environment increased their motor skills and relieved pain. (4) Conclusions: Diving can become one of the forms of rehabilitation for people with disabilities. There is a need for further research to expand our understanding of the benefits and possible health problems involved in diving. These activities have a huge impact on improving the quality of life of people with disabilities.
“This Pandemic Is Making Me More Anxious about My Welfare and the Welfare of Others:” COVID-19 Stressors and Mental Health
Authors: Moore Ramey , Zielinski Melissa J. , Thompson Ronald G. , Willis Don E. , Purvis Rachel S. , McElfish Pearl A.
Published: May 2021

COVID-19 and subsequent social distancing guidelines have changed many aspects of people’s daily lives including the way that they interact within their social environment. Pandemics are inherently social phenomena, and public health measures intended to curtail transmission of COVID-19 (e.g., quarantine and social distancing) have consequences for individuals with anxiety and depression. Using qualitative methods, respondents with previously diagnosed anxiety or depression identified ways in which COVID-19 affected their symptoms at multiple levels of the social ecological model (SEM). Key themes reported were organized following the SEM. Emergent themes at the individual level are isolation/loneliness, fear of contracting COVID-19, and uncertainty about the future. Themes at the interpersonal level are: fears of family contracting COVID-19, separation from family members, and domestic relationships. Themes at the level of community and societal stressors are: employment, community and societal systems, media, and the COVID-19 pandemic. Our findings demonstrate the ways that mental health, physical health/safety, and social environments are interrelated in the experience of COVID-19 for individuals diagnosed with anxiety or depression. These findings make a significant contribution to the literature as this is the first article to document mental health stressors related to the COVID-19 pandemic among individuals with diagnosed anxiety and depressive disorders.
Validity of New Technologies That Measure Bone-Related Dietary and Physical Activity Risk Factors in Adolescents and Young Adults: A Scoping Review
Authors: Davies Alyse , Shi Yumeng , Bauman Adrian , Allman-Farinelli Margaret
Published: May 2021

New technologies may improve the validity of dietary and physical activity assessment and thereby associated findings for lifestyle-related bone health research. This scoping review mapped the evidence for the validity of new technologies that measure bone-related dietary and physical activity risk factors in adolescents and young adults. A systematic literature search was conducted using seven electronic databases for peer-reviewed studies published from January 2008 to 2021. Four studies from four countries were deemed eligible and included in the qualitative synthesis for this review. Two studies assessed diet, reporting the validity or usability of apps. Apps were shown to be a valid tool to measure the dietary intake of vitamin D (r = 0.84) and calcium (r = 0.63). Two studies assessed physical activity and reported the validity of wearable devices to measure impact loading. Hip-worn raw acceleration output correlated positively with ground reaction forces (GRF) for both studies (r range = 0.50–0.87), but wrist-worn accelerations and loading outcomes differed between studies, reporting poor to strong correlations (r range = 0.17–0.87). More research to provide robust evidence concerning validity, reliability, usability and engagement for the use of newer technologies is needed for future diet and physical activity bone research.
Policy Implications for Protecting Health from the Hazards of Fire Smoke. A Panel Discussion Report from the Workshop Landscape Fire Smoke: Protecting Health in an Era of Escalating Fire Risk
Authors: Cowie Christine T. , Wheeler Amanda J. , Tripovich Joy S. , Porta-Cubas Ana , Dennekamp Martine , Vardoulakis Sotiris , Goldman Michele , Sweet Melissa , Howard Penny , Johnston Fay
Published: May 2021

Globally, and nationally in Australia, bushfires are expected to increase in frequency and intensity due to climate change. To date, protection of human health from fire smoke has largely relied on individual-level actions. Recent bushfires experienced during the Australian summer of 2019–2020 occurred over a prolonged period and encompassed far larger geographical areas than previously experienced, resulting in extreme levels of smoke for extended periods of time. This particular bushfire season resulted in highly challenging conditions, where many people were unable to protect themselves from smoke exposures. The Centre for Air pollution, energy and health Research (CAR), an Australian research centre, hosted a two-day symposium, Landscape Fire Smoke: Protecting health in an era of escalating fire risk, on 8 and 9 October 2020. One component of the symposium was a dedicated panel discussion where invited experts were asked to examine alternative policy settings for protecting health from fire smoke hazards with specific reference to interventions to minimise exposure, protection of outdoor workers, and current systems for communicating health risk. This paper documents the proceedings of the expert panel and participant discussion held during the workshop.
Development of Genus-Specific PCR Primers for Molecular Monitoring of Invasive Nostocalean Cyanobacteria
Authors: Kim In-Soo , Park Hae-Kyung , Kim Yong-Jin
Published: May 2021

The geographical range of invasive cyanobacteria with high toxigenic potential is widening because of eutrophication and global warming, thus, monitoring their appearance is necessary for safe water quality control. Most invasive cyanobacteria are nostocalean species, and their accurate identification by classical morphological methods may be problematic. In this study, we developed polymerase chain reaction (PCR) primers to selectively identify five invasive cyanobacterial genera, namely, Chrysosporum, Cuspidothrix, Cylindrospermopsis, Raphidiopsis, and Sphaerospermopsis, using genetic markers such as rbcLX, rpoB, rpoC1, and cpcBA, and determined the amplification conditions for each pair of primers. The primer performances were verified on single or mixed nostocalean cyanobacterial isolates. The five primers allowed selective identification of all the target genera. In field samples collected during summer, when cyanobacteria flourished in the Nakdong River, the respective PCR product was observed in all samples where the target genus was detected by microscopic analysis. Besides, weak bands corresponding to Sphaerospermopsis and Raphidiopsis were observed in some samples in which these genera were not detected by microscopy, suggesting that the cell densities were below the detection limit of the microscopic method used. Thus, the genus-specific primers developed in this study enable molecular monitoring to supplement the current microscopy-based monitoring.
Gender Influences on Physical Activity Awareness of Adolescents and Their Parents
Authors: Lago-Ballesteros Joaquín , García-Pascual Marcos , González-Valeiro Miguel Ángel , Fernández-Villarino María Á.
Published: May 2021

The imbalances between the actual physical activity (PA) of adolescents and the subjective perception both they and their parents have in this regard can play an important role in perpetuating inactive lifestyles. The aim of this study is to analyse these discrepancies by considering gender as a conditioning factor. The participants in the study were 1697 adolescents, 1244 mothers and 1052 fathers in the educational communities of 26 secondary schools located in urban environments of the Autonomous Community of Galicia (Spain). With regard to actual physical activity, a high prevalence of sedentarism (82.1%) was revealed, this being even more acute in girls (87.8%). However, the perceived levels of activity differed significantly from the actual ones with a clear general overestimation both by the adolescents and their parents. When further exploring the data, gender influences were also detected both in adolescent and parental perceptions, since the high rates of overestimation in sedentary individuals were lower in girls and, on the contrary, the low rates of underestimation in active individuals were higher in girls. Moreover, although the level of agreement between actual and perceived physical activity was low overall, with Cohen’s kappa values ranging from 0.006 to 0.047, the lowest values were observed in the case of girls. In conclusion, both the adolescents and their parents were incapable of correctly assessing the actual physical activity of the former, so it seems that the general population lacks knowledge about the amount of physical activity that adolescents need to do to achieve a healthy lifestyle. Consequently, it would be advisable to implement health education campaigns and awareness-raising interventions directed to young people as well as to their parents and, in doing so, gender must be considered by establishing distinct program designs in terms of this variable.
Psychological Impacts and Post-Traumatic Stress Disorder among People under COVID-19 Quarantine and Isolation: A Global Survey
Authors:   TMGH-Global COVID-19 Collaborative
Published: May 2021

Understanding the presence of post-traumatic stress disorder (PTSD) symptoms in quarantined/isolated individuals is essential for decreasing morbidity and mortality caused by the COVID-19 pandemic. However, there is a paucity of evidence quantifying PTSD status globally during confinement in quarantine/isolation facilities during COVID-19. Therefore, we aimed to assess the PTSD status and factors contributing to PTSD development in quarantined/isolated people during pandemic. Using the Impact of Event Scale-Revised (IES-R) scale, our multicentre, multinational, and cross-sectional online survey assessed the psychological impacts on the quarantine/isolation experience of participants suspected or confirmed to have COVID-19, their PTSD status, and various correlates with developing PTSD. We had 944 (35.33%) valid responses (51.1% from females), mostly from Asian countries (635, 71.4%), and 33.9% were healthcare workers. The number of quarantine days in the PTSD symptoms group (using the IES-R cutoff of 24 for symptomatic or full PTSD) was significantly shorter compared to the non-PTSD group (14 (range 14–40) vs. 14 (14–23.75), p = 0.031). Lower rates of PTSD symptoms were observed in participants practicing Buddhist religion than in participants having no religion (OR: 0.30; 95% CI: 0.13–0.68; p = 0.005); individuals with vocational training had a higher risk of developing PTSD symptoms (OR: 2.28 (1.04–5.15); p = 0.043) compared to university graduates. Individuals forced to be quarantined/isolated had higher odds of developing PTSD symptoms than those voluntarily quarantined/isolated (OR: 2.92 (1.84–4.74); p < 0.001). We identified several PTSD correlations among individuals quarantined/isolated during the COVID-19 pandemic, including religious practice, reason for quarantine/isolation, education level, and being a case of the infection. These findings can inform worldwide policies to minimize the adverse effects of such social control measures.
Breast Cancer Predisposition Genes and Synthetic Lethality
Authors: Neiger Hannah E. , Siegler Emily L. , Shi Yihui
Published: May 2021

BRCA1 and BRCA2 are tumor suppressor genes with pivotal roles in the development of breast and ovarian cancers. These genes are essential for DNA double-strand break repair via homologous recombination (HR), which is a virtually error-free DNA repair mechanism. Following BRCA1 or BRCA2 mutations, HR is compromised, forcing cells to adopt alternative error-prone repair pathways that often result in tumorigenesis. Synthetic lethality refers to cell death caused by simultaneous perturbations of two genes while change of any one of them alone is nonlethal. Therefore, synthetic lethality can be instrumental in identifying new therapeutic targets for BRCA1/2 mutations. PARP is an established synthetic lethal partner of the BRCA genes. Its role is imperative in the single-strand break DNA repair system. Recently, Olaparib (a PARP inhibitor) was approved for treatment of BRCA1/2 breast and ovarian cancer as the first successful synthetic lethality-based therapy, showing considerable success in the development of effective targeted cancer therapeutics. Nevertheless, the possibility of drug resistance to targeted cancer therapy based on synthetic lethality necessitates the development of additional therapeutic options. This literature review addresses cancer predisposition genes, including BRCA1, BRCA2, and PALB2, synthetic lethality in the context of DNA repair machinery, as well as available treatment options.
Urolithin and Reduced Urolithin Derivatives as Potent Inhibitors of Tyrosinase and Melanogenesis: Importance of the 4-Substituted Resorcinol Moiety
Authors: Lee Sanggwon , Choi Heejeong , Park Yujin , Jung Hee Jin , Ullah Sultan , Choi Inkyu , Kang Dongwan , Park Chaeun , Ryu Il Young , Jeong Yeongmu , Hwang YeJi , Hong Sojeong , Chun Pusoon , Moon Hyung Ryong
Published: May 2021

We previously reported (E)-β-phenyl-α,β-unsaturated carbonyl scaffold ((E)-PUSC) played an important role in showing high tyrosinase inhibitory activity and that derivatives with a 4-substituted resorcinol moiety as the β-phenyl group of the scaffold resulted in the greatest tyrosinase inhibitory activity. To examine whether the 4-substituted resorcinol moiety could impart tyrosinase inhibitory activity in the absence of the α,β-unsaturated carbonyl moiety of the (E)-PUSC scaffold, 10 urolithin derivatives were synthesized. To obtain more candidate samples, the lactone ring in synthesized urolithins was reduced to produce nine reduced urolithins. Compounds 1c (IC50 = 18.09 ± 0.25 μM), 1h (IC50 = 4.14 ± 0.10 μM), and 2a (IC50 = 15.69 ± 0.40 μM) had greater mushroom tyrosinase-inhibitory activities than kojic acid (KA) (IC50 = 48.62 ± 3.38 μM). The SAR results suggest that the 4-substituted resorcinol motif makes an important contribution to tyrosinase inhibition. To investigate whether these compounds bind to human tyrosinase, a human tyrosinase homology model was developed. Docking simulations with mushroom and human tyrosinases showed that 1c, 1h, and 2a bind to the active site of both tyrosinases with higher binding affinities than KA. Pharmacophore analyses showed that two hydroxyl groups of the 4-substituted resorcinol entity act as hydrogen bond donors in both mushroom and human tyrosinases. Kinetic analyses indicated that these compounds were all competitive inhibitors. Compound 2a inhibited cellular tyrosinase activity and melanogenesis in α-MSH plus IBMX-stimulated B16F10 melanoma cells more strongly than KA. These results suggest that 2a is a promising candidate for the treatment of skin pigment disorders, and show the 4-substituted resorcinol entity importantly contributes to tyrosinase inhibition.
The Quorum Sensing Auto-Inducer 2 (AI-2) Stimulates Nitrogen Fixation and Favors Ethanol Production over Biomass Accumulation in Zymomonas mobilis
Authors: Alencar Valquíria Campos , Silva Juliana de Fátima dos Santos , Vilas Boas Renata Ozelami , Farnézio Vinícius Manganaro , de Maria Yara N. L. F. , Aciole Barbosa David , Almeida Alex Tramontin , de Souza Emanuel Maltempi , Müller-Santos Marcelo , Jabes Daniela L. , Menegidio Fabiano B. , Costa de Oliveira Regina , Rodrigues Tiago , Tersariol Ivarne Luis dos Santos , Walmsley Adrian R. , Nunes Luiz R.
Published: May 2021

Autoinducer 2 (or AI-2) is one of the molecules used by bacteria to trigger the Quorum Sensing (QS) response, which activates expression of genes involved in a series of alternative mechanisms, when cells reach high population densities (including bioluminescence, motility, biofilm formation, stress resistance, and production of public goods, or pathogenicity factors, among others). Contrary to most autoinducers, AI-2 can induce QS responses in both Gram-negative and Gram-positive bacteria, and has been suggested to constitute a trans-specific system of bacterial communication, capable of affecting even bacteria that cannot produce this autoinducer. In this work, we demonstrate that the ethanologenic Gram-negative bacterium Zymomonas mobilis (a non-AI-2 producer) responds to exogenous AI-2 by modulating expression of genes involved in mechanisms typically associated with QS in other bacteria, such as motility, DNA repair, and nitrogen fixation. Interestingly, the metabolism of AI-2-induced Z. mobilis cells seems to favor ethanol production over biomass accumulation, probably as an adaptation to the high-energy demand of N2 fixation. This opens the possibility of employing AI-2 during the industrial production of second-generation ethanol, as a way to boost N2 fixation by these bacteria, which could reduce costs associated with the use of nitrogen-based fertilizers, without compromising ethanol production in industrial plants.
A Fair Assessment of Evaluation Tools for the Murine Microbead Occlusion Model of Glaucoma
Authors: Claes Marie , Santos Joana R. F. , Masin Luca , Cools Lien , Davis Benjamin M. , Arckens Lutgarde , Farrow Karl , De Groef Lies , Moons Lieve
Published: May 2021

Despite being one of the most studied eye diseases, clinical translation of glaucoma research is hampered, at least in part, by the lack of validated preclinical models and readouts. The most popular experimental glaucoma model is the murine microbead occlusion model, yet the observed mild phenotype, mixed success rate, and weak reproducibility urge for an expansion of available readout tools. For this purpose, we evaluated various measures that reflect early onset glaucomatous changes in the murine microbead occlusion model. Anterior chamber depth measurements and scotopic threshold response recordings were identified as an outstanding set of tools to assess the model’s success rate and to chart glaucomatous damage (or neuroprotection in future studies), respectively. Both are easy-to-measure, in vivo tools with a fast acquisition time and high translatability to the clinic and can be used, whenever judged beneficial, in combination with the more conventional measures in present-day glaucoma research (i.e., intraocular pressure measurements and post-mortem histological analyses). Furthermore, we highlighted the use of dendritic arbor analysis as an alternative histological readout for retinal ganglion cell density counts.
Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
Authors: Kasimova Anastasiya A. , Arbatsky Nikolay P. , Tickner Jacob , Kenyon Johanna J. , Hall Ruth M. , Shneider Michael M. , Dzhaparova Alina A. , Shashkov Alexander S. , Chizhov Alexander O. , Popova Anastasiya V. , Knirel Yuriy A.
Published: May 2021

Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.
Kawasaki Disease Patient Stratification and Pathway Analysis Based on Host Transcriptomic and Proteomic Profiles
Authors: Jackson Heather , Menikou Stephanie , Hamilton Shea , McArdle Andrew , Shimizu Chisato , Galassini Rachel , Huang Honglei , Kim Jihoon , Tremoulet Adriana , Thorne Adam , Fischer Roman , de Jonge Marien , Kuijpers Taco , Wright Victoria , Burns Jane , Casals-Pascual Climent , Herberg Jethro , Levin Mike , Kaforou Myrsini ,   on behalf of the PERFORM Consortium
Published: May 2021

The aetiology of Kawasaki disease (KD), an acute inflammatory disorder of childhood, remains unknown despite various triggers of KD having been proposed. Host ‘omic profiles offer insights into the host response to infection and inflammation, with the interrogation of multiple ‘omic levels in parallel providing a more comprehensive picture. We used differential abundance analysis, pathway analysis, clustering, and classification techniques to explore whether the host response in KD is more similar to the response to bacterial or viral infections at the transcriptomic and proteomic levels through comparison of ‘omic profiles from children with KD to those with bacterial and viral infections. Pathways activated in patients with KD included those involved in anti-viral and anti-bacterial responses. Unsupervised clustering showed that the majority of KD patients clustered with bacterial patients on both ‘omic levels, whilst application of diagnostic signatures specific for bacterial and viral infections revealed that many transcriptomic KD samples had low probabilities of having bacterial or viral infections, suggesting that KD may be triggered by a different process not typical of either common bacterial or viral infections. Clustering based on the transcriptomic and proteomic responses during KD revealed three clusters of KD patients on both ‘omic levels, suggesting heterogeneity within the inflammatory response during KD. The observed heterogeneity may reflect differences in the host response to a common trigger, or variation dependent on different triggers of the condition.
IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
Authors: Lee Seounghun , Shin Hyo-Jung , Noh Chan , Kim Song-I , Ko Young-Kwon , Lee Sun-Yeul , Lim Chaeseong , Hong Boohwi , Yang Sin-Young , Kim Dong-Woon , Lee Won-Hyung , Kim Yoon-Hee
Published: May 2021

Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to break down IκB, which blocks the activity of NF-kB. Therefore, it prevents the activity of NK-kB. This study investigated whether neuropathic pain can be reduced in spinal nerve ligation (SNL) rats by reducing the activity of microglia by delivering IKBKB small interfering RNA (siRNA)-encapsulated poly (lactic-co-glycolic acid) (PLGA) nanoparticles. PLGA nanoparticles, as a carrier for the delivery of IKBKB genes silencer, were used because they have shown potential to enhance microglial targeting. SNL rats were injected with IKBKB siRNA-encapsulated PLGA nanoparticles intrathecally for behavioral tests on pain response. IKBKB siRNA was delivered for suppressing the expression of IKBKB. In rats injected with IKBKB siRNA-encapsulated PLGA nanoparticles, allodynia caused by mechanical stimulation was reduced, and the secretion of pro-inflammatory mediators due to NF-κB was reduced. Delivering IKBKB siRNA through PLGA nanoparticles can effectively control the inflammatory response and is worth studying as a treatment for neuropathic pain.
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing
Authors: Kaczmarek-Szczepańska Beata , Ostrowska Justyna , Kozłowska Justyna , Szota Zofia , Brożyna Anna A. , Dreier Rita , Reiter Russel J. , Slominski Andrzej T. , Steinbrink Kerstin , Kleszczyński Konrad
Published: May 2021

The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine.
Dysregulation of Astrocyte Ion Homeostasis and Its Relevance for Stroke-Induced Brain Damage
Authors: van Putten Michel J. A. M. , Fahlke Christoph , Kafitz Karl W. , Hofmeijer Jeannette , Rose Christine R.
Published: May 2021

Ischemic stroke is a leading cause of mortality and chronic disability. Either recovery or progression towards irreversible failure of neurons and astrocytes occurs within minutes to days, depending on remaining perfusion levels. Initial damage arises from energy depletion resulting in a failure to maintain homeostasis and ion gradients between extra- and intracellular spaces. Astrocytes play a key role in these processes and are thus central players in the dynamics towards recovery or progression of stroke-induced brain damage. Here, we present a synopsis of the pivotal functions of astrocytes at the tripartite synapse, which form the basis of physiological brain functioning. We summarize the evidence of astrocytic failure and its consequences under ischemic conditions. Special emphasis is put on the homeostasis and stroke-induced dysregulation of the major monovalent ions, namely Na+, K+, H+, and Cl-, and their involvement in maintenance of cellular volume and generation of cerebral edema.
Species Diversity in the Leafhopper Genus Batracomorphus Lewis (Hemiptera: Cicadellidae: Iassinae) from Qinling Mountain in Shaanxi
Authors: Hu Yulin , Gao Lili , Han Zihan , Dai Wu
Published: May 2021

The genus Batracomorphus Lewis is the third largest leafhopper genus in the world, with its greatest diversity in the Oriental region. Here, nine species of Batracomorphus, including one new species, are recorded from Shaanxi Province, China, for the first time: B. allionii (Turton), B. clavatus Cai and Shen, B. fletcheri Hu and Dai sp. nov., B. geminatus (Li and Wang), B. juno Knight, B. lateprocessus Li and Wang, B. lunatus Cai and He, B. subfuscus (Li and Wang) and B. pandarus Knight. Among them, B. juno Knight is recorded from China for the first time. One new synonym is revealed: B.nigromarginattus Cai and Shen, 1999 is a junior synonym of B. subfuscus (Li and Wang, 1993). All taxa are described, and photographs of male genitalia are given based on observations of specimens from Qinling Mountain in Shaanxi of China. A key to the species found in Qinling Mountain of Shaanxi is also provided.
Mitochondrial Phylogenomics of Tenthredinidae (Hymenoptera: Tenthredinoidea) Supports the Monophyly of Megabelesesinae as a Subfamily
Authors: Niu Gengyun , Jiang Sijia , Doğan Özgül , Korkmaz Ertan Mahir , Budak Mahir , Wu Duo , Wei Meicai
Published: May 2021

Tenthredinidae represents one of the external feeders of the most diverse superfamily, Tenthredinoidea, with diverse host plant utilization. In this study, four complete mitochondrial genomes (mitogenomes), those of Cladiucha punctata, Cladiucha magnoliae, Megabeleses magnoliae, and Megabeleses liriodendrovorax, are newly sequenced and comparatively analyzed with previously reported tenthredinid mitogenomes. The close investigation of mitogenomes and the phylogeny of Tenthredinidae leads us to the following conclusions: The subfamilial relationships and phylogenetic placements within Tenthredinidae are mostly found to be similar to the previously suggested phylogenies. However, the present phylogeny supports the monophyly of Megabelesesinae as a subfamily, with the sister-group placement of Cladiucha and Megabeleses outside of Allantinae. The occurrence of the same type of tRNA rearrangements (MQI and ANS1ERF) in the mitogenomes of Megabelesesinae species and the presence of apomorphic morphological characters also provide robust evidence for this new subfamily. The divergence and diversification times of the subfamilies appear to be directly related to colonization of the flowering plants following the Early Cretaceous. The origin time and diversification patterns of Megabelesesinae were also well matched with the divergence times of their host plants from Magnoliaceae.
Successional Development of Fungal Communities Associated with Decomposing Deadwood in a Natural Mixed Temperate Forest
Authors: Lepinay Clémentine , Jiráska Lucie , Tláskal Vojtěch , Brabcová Vendula , Vrška Tomáš , Baldrian Petr
Published: May 2021

Deadwood represents an important carbon stock and contributes to climate change mitigation. Wood decomposition is mainly driven by fungal communities. Their composition is known to change during decomposition, but it is unclear how environmental factors such as wood chemistry affect these successional patterns through their effects on dominant fungal taxa. We analysed the deadwood of Fagus sylvatica and Abies alba across a deadwood succession series of >40 years in a natural fir-beech forest in the Czech Republic to describe the successional changes in fungal communities, fungal abundance and enzymatic activities and to link these changes to environmental variables. The fungal communities showed high levels of spatial variability and beta diversity. In young deadwood, fungal communities showed higher similarity among tree species, and fungi were generally less abundant, less diverse and less active than in older deadwood. pH and the carbon to nitrogen ratio (C/N) were the best predictors of the fungal community composition, and they affected the abundance of half of the dominant fungal taxa. The relative abundance of most of the dominant taxa tended to increase with increasing pH or C/N, possibly indicating that acidification and atmospheric N deposition may shift the community composition towards species that are currently less dominant.
Biocontrol Effects of Paecilomyces variotii against Fungal Plant Diseases
Authors: Moreno-Gavíra Alejandro , Diánez Fernando , Sánchez-Montesinos Brenda , Santos Mila
Published: May 2021

The genus Paecilomyces is known for its potential application in the control of pests and diseases; however, its use in agriculture is limited to few species. Research interest in new formulations based on microorganisms for the control of pathogens is growing exponentially; therefore, it is necessary to study new isolates, which may help control diseases effectively, and to examine their compatibility with established agricultural control methods. We analysed in vitro and in vivo the antagonistic capacity of Paecilomyces variotii against seven phytopathogens with a high incidence in different crops, and we examined its compatibility with 24 commercial fungicides. P. variotii was applied in the following pathosystems: B. cinereal—melon, Sclerotinia sclerotiorum—pepper, R. solani—tomato, F. solani—zucchini, P. aphanidermatum—melon, M. melonis—melon, and P. xanthii—zucchini. The results showed strong control effects on M. melonis and P. xanthii, reducing the disease severity index by 78% and 76%, respectively. The reduction in disease severity in the other pathosystems ranged from 29% to 44%. However, application of metabolites alone did not cause any significant effect on mycelial growth of phytopathogens, apart from F. solani, in which up to 12% inhibition was observed in vitro when the extract was applied at a concentration of 15% in the medium. P. variotii was compatible with most of the tested fungicides, and of the 24 fungicides tested at the maximum authorised dose, 6 acted as fungicides, 4 as fungistatics, and the remaining showed inhibition rates ranging from 18.2% to 95.8%. These results indicate that P. variotii is a potential biological control agent to be used against several aerial and soil diseases, thus it should be integrated into modern pest management strategies.
Neutrophil Cells Are Essential for The Efficacy of a Therapeutic Vaccine against Paracoccidioidomycosis
Authors: Dias Lucas dos Santos , Silva Leandro B. R. , Nosanchuk Joshua D. , Taborda Carlos Pelleschi
Published: May 2021

Paracoccidioidomycosis (PCM), caused by the Paracoccidioides species, is a systemic disease endemic in several Latin American countries, mainly in Brazil, Colombia, Argentina, and Venezuela. Current treatment approaches are challenging as they require prolonged durations of antifungal drugs that have potential toxicities, and despite antifungals, relapses are common. Hence, new therapeutic approaches, such as vaccines, are being investigated. The therapeutic vaccine consisting of peptide P10 associated with lipid cationic DODAB (P10+DODAB) is effective in murine models of PCM. However, the specific immune mechanisms required for the protective response has not been fully elucidated. The present work aims at evaluating the participation of neutrophils in the immune response induced by P10+DODAB. We found that the vaccine reduced both the influx of pulmonary neutrophils and the fungal load in comparison to infected animals that did not receive this treatment. The parenchymal architecture of the lungs of P10+DODAB-treated animals was largely preserved with only a few granulomas present, and tissue cytokine analysis showed a Th1 cytokine profile with augmented levels of IL-12, IFN-γ and TNF-α, and low levels of IL-4. When neutrophils were depleted 24 h prior to each treatment, the effectiveness of the P10+DODAB vaccine was completely lost as the fungal burdens remained high and histological examination showed a marked inflammation and fungal dissemination with a dysregulated cytokine response. In conclusion, these findings indicate that neutrophils are vital to ensure the triggering of an effective immune response to P10+DODAB.
Simultaneous Removal of Pb2+ and Zn2+ Heavy Metals Using Fly Ash Na-X Zeolite and Its Carbon Na-X(C) Composite
Authors: Panek Rafał , Medykowska Magdalena , Wiśniewska Małgorzata , Szewczuk-Karpisz Katarzyna , Jędruchniewicz Katarzyna , Franus Małgorzata
Published: May 2021

Pure zeolite (Na-X) and a zeolite–carbon composite (Na-X(C)) were investigated as adsorbents of heavy metals—Pb2+ and Zn2+ from an aqueous solution. These materials were synthesized from fly ash—a waste from conventional hard coal combustion. Both solids were characterized using XRD, SEM-EDS, nitrogen adsorption/desorption, particle size and elemental composition analyses. The adsorption study was performed at pH 5 in the systems containing one or two adsorbates simultaneously. The obtained results showed that the pure zeolite was characterized by a more developed surface area (728 m2/g) than its carbon composite (272 m2/g), and the mean pore diameters were equal to 1.73 and 2.56 nm, respectively. The pure Na-X zeolite showed better adsorption properties towards heavy metals than its Na-X(C) composite, and Zn2+ adsorbed amounts were significantly higher than the Pb2+ ones (the highest experimental adsorption levels were: for Zn2+—656 and 600 mg/g, and for Pb2+—575 and 314 mg/g, on the Na-X and Na-X(C) surfaces, respectively). The zinc ions are exchanged with the cations inside the zeolite materials structure more effectively than lead ions with a considerably larger size. In the mixed systems, the competition between both heavy metals for access to the active sites on the adsorbent surface leads to the noticeable reduction in their adsorbed amounts. Moreover, the hydrochloric acid was a better desorbing agent for both heavy metals, especially Pb2+ one (desorption reached 78%), than sodium base (maximal desorption 25%).
Impact of Sandblasting on Morphology, Structure and Conductivity of Zirconia Dental Ceramics Material
Authors: Jakovac Marko , Klaser Teodoro , Radatović Borna , Bafti Arijeta , Skoko Željko , Pavić Luka , Žic Mark
Published: May 2021

Over the last decade, zirconia (ZrO2)-based ceramic materials have become more applicable to modern dental medicine due to the sustained development of diverse computer-aided design/computer-aided manufacturing (CAD/CAM) systems. However, before the cementation and clinical application, the freshly prepared zirconia material (e.g., crowns) has to be processed by sandblasting in the dental laboratory. In this work, the impact of the sandblasting on the zirconia is monitored as changes in morphology (i.e., grains and cracks), and the presence of impurities might result in a poor adhesive bonding with cement. The sandblasting is conducted by using Al2O3 powder (25, 50, 110 and 125 µm) under various amounts of air-abrasion pressure (0.1, 0.2, 0.4 and 0.6 MPa). There has been much interest in both the determination of the impact of the sandblasting on the zirconia phase transformations and conductivity. Morphology changes are observed by using Scanning Electron Microscope (SEM), the conductivity is measured by Impedance Spectroscopy (IS), and the phase transformation is observed by using Powder X-Ray Diffraction (PXRD). The results imply that even the application of the lowest amount of air-abrasion pressure and the smallest Al2O3 powder size yields a morphology change, a phase transformation and a material contamination.