bluuebunny/crossref_metadata_2025 huggingface dataset:
Abstract 674: Vesselin Controls Vascular Morphogenesis by Activating Small Gtpases in Endothelial Cells
Authors: Wang Yanling , He Zhengqiang , Duan Hao , Huang Menggui , Fan Yi , Gong Yanqing A
Published: March 2019

Vascularization is a fundamental process in development, wound healing, and the progression of cardiovascular diseases. Formation of new blood vessels with functional and structural integrity is crucial for organ growth as well as post-injury tissue repair and regeneration. Although vascular sprouting and outgrowth,
i.e.
, angiogenesis, has been extensively studied, the underlying mechanisms for vessel formation remain largely unexplored. Here we reveal a critical role of phosphatidylinositol 4,5-bisphosphate (PIP2) for vessel formation in endothelial cells (ECs). Moreover, by using mass spectrometry, our identified Tbc1d2b as a novel PIP2-binding protein in vessel-formatting ECs. Considering its critical function in vessel formation, we name this protein “vesselin”. We showed that vesselin preferentially localizes to blood vessels in various human tissues, and that its siRNA-mediated knockdown inhibits EC tube formation, supporting its major role in vessel formation. Remarkably, injection of inhibitory vesselin morpholino into the embryos blocks blood vessel formation and zebrafish development. Vesselin contains PH (pleckstrin homology) and TBC (Tre2/Bub2/Cdc16) domains, which are known to bind phosphatidylinositols and display GTPase-activating protein (GAP) activities towards Rab GTPases, respectively. Consistently, we found by mass spectrometry that vesselin interacts with the small GTPase Rab13 and small GTPase Rac1. Finally, our further mechanistic studies showed that vesselin interacts with Rac1 and Rab13 during EC vessel formation, and regulates their activity in a PIP2-dependent manner. Thus, we uncover a previously unidentified regulatory system for EC vessel formation, providing molecular resolution of vascular morphogenesis. Targeting vesselin may provide new therapeutic strategies in cardiovascular diseases.
Abstract 680: Specific Deletion of SHP-1 in Smooth Muscle Cells Restores PDGF Action in Diabetes
Authors: Robillard Stéphanie , Lizotte Farah , Geraldes Pedro M
Published: March 2019

Introduction:
Ischemia due to narrowing of the femoral artery and distal vessels is also a major cause of peripheral arterial disease and morbidity affecting patients with diabetes. Our laboratory has previously shown that hyperglycemia reduced platelet-derived growth factor (PDGF) activity in ischemic muscle of diabetic mice, which was associated with increased SHP-1 expression, a protein tyrosine phosphatase. The objective of this study is to evaluate the impact of SHP-1 deletion in smooth muscle cells both in vitro and in vivo.

Methods:
Non-diabetic (NDM) and 3 months diabetic (DM) mice with deletion of SHP-1 specifically in smooth muscle cells (SMC) were used. Ligation of the femoral artery was performed and blood flow reperfusion was measured by laser Doppler for 4 weeks. Primary SMC were exposed to normal (5.6mM; NG) or high glucose concentrations (25mM; HG) for 48h, in normoxia (20% oxygen) or hypoxia (1%) for the last 24h in presence of PDGF, a pro-angiogenic factor.

Results:
Blood flow was recovered to 47% in DM mice compared to 80% in NDM mice. Specific SMC deletion of SHP-1 enhanced reperfusion in NDM and DM mice up to 78% and 67%, respectively. In culture, PDGF-induced proliferation, migration, and Akt phosphorylation were reduced by 69%, 50% and 40%, respectively in SMC exposed to HG+hypoxia. Inhibition of PDGF actions was associated with increased SHP-1 phosphatase activity (40%) and enhanced interaction of SHP-1 with the PDGF receptor-β (5.6-fold). Overexpression of the dominant negative form of SHP-1 restored PDGF-induced proliferation and migration as well as Akt and ERK phosphorylation in SMC exposed to HG+hypoxia.

Conclusion:
High glucose level induced SHP-1 activity and caused inhibition of PDGF pro-angiogenic actions in SMC, whereas the deletion of SHP-1 specifically in SMC restored blood flow reperfusion in diabetes.
Abstract 688: Differential Matricellular Protein (CCN) Expression in Atherosclerotic and Aneurysmal Smooth Muscle Cells
Authors: Polcz Monica , Airhart Nathan , Marshall Monique , Curci John
Published: March 2019

Introduction:
Recent evidence implicates reduced activity of CCN3 as a mediator of MMP production and aortic wall degeneration in the process of model aortic aneurysm. We have previously shown that AAA-derived VSMC have increased elastolytic activity. We hypothesized that there would be differential CCN expression and production by VSMC derived from human AAA or derived from human atherosclerotic plaque.

Results:
Early passage cell lines of VSMC lineage as confirmed by flow cytometry were analyzed by Illumina expression array. The VSMC were derived from infrarenal aneurysm (AAA, n=22) or atherosclerotic plaque (Pl, n=29). There were no differences in expression of CCN1, 2, 5 or 6 (Fig A) between the two cell types. Expression of CCN3 was significantly reduced in AAA compared to Pl, CCN4 was significantly greater in AAA than in Pl-derived VSMC. Western blot of cellular extracts from these cells (n=3 each) confirms qualitatively greater CCN3 in Pl vs AAA VSMC, while no appreciable difference was seen in the production of CCN4 (Fig B). Production of CCN1, 2 and 6 are qualitatively similar and there may be slightly lower CCN5 production in Pl vs AAA-derived VSMC (Data not shown).

Matricellular protein expression show distinct patterns between Plaque-derived and AAA-derived VSMC and may be related to the enhanced elastolytic activity of cells in AAA.
Abstract 698: Functional Analysis of Smad3 Deficiency in VSMCs Derived From Crispr/cas9-modified Human Induced Pluripotent Stem Cells
Authors: Gong Jian , Qiu Ping , Chen Eugene , Yang Bo
Published: March 2019

Introduction:
Transcription factor Smad3 plays key roles in both TGF-β signaling transduction and vascular smooth muscle cells(VSMCs) differentiation. SMAD3 gene mutations cause aneurysms-osteoarthritis syndrome(AOS), an autosomal dominant genetic disease with high rate occurrence of thoracic aortic aneurysm(TAA) and early-onset osteoarthritis. The production of patient-derived hiPSCs and genomic-modified ihPSCs by using Crispr/Cas9 technique provide a promise for in vitro disease modeling of SMAD3 mutation in VSMCs as well as drug screening for potential therapeutic targets.

Hypothesis:
We hypothesis that introducing SMAD3 mutations into hIPSCs that mimic the identified genotypic alteration in AOS patients could recapture the phenotypic defects of differentiation, contractibility and extracellular matrix(ECM) of VSMCs derived from hIPSCs-differentiated neural crest stem cells(NCSCs), the embryonic origin of VSMCs for both aorta root and ascending aorta.

Method:
hIPSCs were generated from non-TAA patients’ peripheral blood mononuclear cells by overexpression of Yamanaka factors. The indel mutation targeting SMAD3 exon5 were induced by using Crispr/Cas9 technique, which form frameshift mutation close to one in identified AOS patients. Selected cell clones of genomic modified hIPSCs were differentiated into NCSCs-derived VSMCs. Comparing analysis of VSMCs function were proceed in both hIPSCs lines with or without SMAD3 mutation.

Result:
We generated a homozygote with SMAD3 frameshift mutation. It contains a 7-base pair deletion(c.633_639delGATGTCC), which is similar with a pathogenic Smad3 mutation (c.652 delA). This mutation leads to a premature stop codon and remove the Smad3 protein. The qRCR and WB result showed that the expression of ACTA2 decreased in the differentiated SMC with SMAD3 mutation. Also, the expression of elastin, collagen type III were significantly down-regulated in the SMAD3 mutation cell line while the MMP9 was up-regulated.

Conclusion:
SMAD3 mutation may cause defects of differentiation, contractibility and ECM synthesis of VSMCs derived from NCSC.
Abstract 699: Endothelial Mineralocorticoid Receptor and Neutrophils Mediate Aldo plus Salt-Induced Abdominal Aortic Aneurysm
Authors: Liu Shu , Zhong Yu , Guo Zhenheng , Gong Ming
Published: March 2019

Background:
We recently reported that administration of mice with Aldo or deoxycorticosterone acetate (DOCA) plus salt induces abdominal aortic aneurysm (AAA) and treatment of mice with mineralocorticoid receptor (MR) blockers abolished Aldo plus salt-induced AAA. However, the mechanism by which Aldo plus salt induces AAA is largely unknown.

Methods:
A tamoxifen inducible EC (EC)-specific MR knockout mouse model (iECMRKO) was developed. ECMRKO mice, and control mice were administered with Aldo or DOCA plus sat salt to induce AAA. C57BL/6 mice were injected with anti-mouse polymorphonuclear (PMN) leukocytes antibody to deplete neutrophils. The aortic internal and external diameter, the aortic dilation rate, and the incidence of AAA were determined.

Results:
Selective genetic deletion of MR from ECs, protects mice from Aldo or DOCA plus salt-induced AAA. EC-specific MR deletion had little effect on Aldo plus salt-induced sodium retention, hypertension, and renal fibrosis, but largely suppressed aortic elastin degradation, matrix metallopeptidase (MMP) 2 and MMP9 upregulation, macrophage and neutrophil infiltration. Surprisingly, neutrophil, but not macrophage, was observed in aorta from control mice, but not iECMRKO mice, indicating that neutrophils but not macrophages is involved in the early processes of Aldo plus salt-induced and endothelia MR-mediated AAA development. Treatment of C57BL/6 mice with of anti- PMN antibody selectively suppressed DOCA plus salt-induced circulating Ly6G-postive neutrophils, but not CD4-postive leukocytes, protected mice from DOCA plus salt-induced AAA. Aldo-induced endothelial adhesion molecule (E-selectin, P-selectin, ICAMP1, and VCAM-1) and proinflammatory cytokine (IL-6 and MCP-1) mRNA expressions were abolished in cultured MR-deficient ECs. Aldo plus salt-induced ICAM-1 but not VCAM-1 protein upregulation was abolished in aorta from ECMRKO mice.

Conclusions:
Our results demonstrate a significant role of endothelial MR, in Aldo plus salt-induced AAA. Moreover, our results also reveal a provocative mechanism by which ICAM-1, but not VCAM-1, and neutrophils, but not macrophages, mediates the early processes of Aldo plus salt-induced and endothelia MR-mediated AAA development.
Abstract 711: Gut Bacteria Influences the Growth of Abdominal Aortic Aneurysms in Mice
Authors: Stines Ian , Hyoju Sanjiv , Zaborina Olga , Alverdy John , Babrowski Trissa
Published: March 2019

Objectives:
The goal of this study was to determine if highly collagenolytic bacteria introduced to the gut microbiome induces abdominal aortic aneurysm in mice.

Methods:
C57BL/6 mice received a prolonged 4-day course of preoperative antibiotics (oral clindamycin and subcutaneous cefoxitin) and received pre- and postoperative enemas containing either 10% glycerol solution (control) or a freshly prepared bacterial suspension with a highly collagenolytic strain of
Serratia marcescens
(S2). All mice underwent laparotomy, aortic crush injury with forceps, and application of either 0.9% sodium chloride (control) or 10% calcium chloride to the periadventitial aortic space. Aortic diameter was measured on initial exposure and on sacrifice on postoperative day 21. Groups were compared using a series of Student’s T-test.

Results:
On postoperative day 21, the difference in aortic diameter for mice treated with periadventitial sodium chloride and glycerol enema was minimal (control 1, n=6, 0.03 mm ± 0.30). In mice treated with periadventitial calcium chloride and glycerol enema, fusiform aneurysm was reliably produced compared to control 1 (control 2, n=6, 0.53 mm ± 0.30, p ≤ 0.05). In mice treated with periadventitial sodium chloride and S2 enema, fusiform aneurysm was reliably produced with a significant difference in diameter compared to control 1 (n=8, 0.63 mm ± 0.30, p ≤ 0.05). In mice treated with periadventitial calcium chloride and S2 enema, fusiform aneurysm was reliably produced with a significant difference in diameter compared to control 1 (n=8, 0.59 mm ± 0.30, p ≤ 0.05). There was no significant difference between the 2 treatment groups.

Conclusions:
The introduction of highly collagenolytic
Serratia marcescens
to the gut microbiome of mice in addition to aortic crush injury reliably produces fusiform aortic aneurysm in this model. Further investigation is required to elucidate the mechanism of aneurysm formation.
Abstract 712: Elastin Derived Peptides Augments Abdominal Aortic Aneurysm Formation
Authors: Stubbe Jane , Bjørnes Nelly , Pham Tu Quyen , Kurtzhals Anne Katrine , Griepke Dam Nielsen Silke , Melholt Rasmussen Lars , Sanddal Lindholt Jes
Published: March 2019

Abdominal aortic aneurysm AAA is a chronic dilatation of the abdominal aorta. It is a potentially life threatening disease as it develops asymptomatic and is often first discovered at the time of rupture. One key element in AAA formation is degradation of the elastin fibers in the aortic wall. When elastin is degraded small bioactive elastin derived peptides (EDPs) are released into the circulation. These EDPs affect vascular smooth muscle cells in the aortic wall and attract monocytes to the aortic wall resulting in further degradation of elastin. We hypothesize that the level of circulating elastin derived peptides (EDPs) augments AAA expansion and that inhibition of circulating EDPs will inhibit AAA expansion. We tested the hypothesis in an elastase induced aneurysm murine model by applying synthetic EDPs or scrambles peptides and measure the AAA size. In addition, EDP-neutralizing antibody or control IgG was given to angiotensin II induced AAA in ApoE-/- mice and AAA size was determined 28 days later. Fourteen days after elastase infusion the abdominal aortic diameter was significantly increased in the EDP treated group when compared to the scramble peptid treated control group (1.33±0.07 vs. 1.13±0.06 mm, n=17-19, p<0.05). Inhibition of circulating EDPs by EDP neutralizing antibodies when compared to IgG controls showed a clear trend toward decreased AAA expansion based on outer aortic diameter (1.4 ±0.1 vs. 2.2 ±0.4 mm, n=8-9, p=0.059) and wet weights of the abdominal aorta (median: 9.2 vs. 22.1 mg, n=7-9, p=0.07) 28 days after angiotensin II infusion in ApoE-/- mice. In the aneurysmal wall of the mice treated with elastin neutralizing antibody, there was less ruptured elastin, and there was significantly fewer infiltrating of CD45 positive cells, while CD206 positive anti-inflammatory M2 macrophages were significantly augmented when compared to IgG treated controls (n=7-9).In conclusion, EDPs augment abdominal aortic aneurysms. Thus, inhibition of circulating EDPs shows great potential against AAA expansion.
Is Prevalence of Atherosclerotic Risk Factors Increasing Among Young Adults? It Depends on How You Ask
Authors: Leppert Michelle H. , Poisson Sharon N. , Sillau Stefan H. , Campbell Jonathan D. , Ho P. Michael , Burke James F.
Published: March 2019

Background

Incidence of cardiovascular disease in young adults is unabated. Increased prevalence of self‐reported atherosclerotic risk factors may be driving this trend. The goal of this study was to examine whether the prevalence of atherosclerotic risk factors in young adults is increasing over time using both self‐report and standard clinical criteria.

Methods and Results

Data from young adults, aged 20 to 45 years, in the National Health and Nutrition Examination Survey from 1999/2000 to 2013/2014 were analyzed. Risk factor prevalence of hypertension, diabetes mellitus, and hyperlipidemia was measured using clinical criteria and self‐report. Smoking was based on self‐report only, and obesity was based clinically on body mass index and waist to height ratio. Prevalence by survey was adjusted for age, sex, and race/ethnicity. By clinical criteria, adjusted prevalence of any 3 risk factors (hypertension, diabetes mellitus, and hyperlipidemia) declined slightly from 21.8% to 18.9% (
P
for trend=0.05). However, by self‐report, the adjusted prevalence of any 3 risk factors increased from 17.8% to 26.5% (
P
<0.01). Hypertension was unchanged by clinical criteria (
P
=0.32) but increased by self‐report (
P
<0.08). Diabetes mellitus, by clinical diagnosis and self‐report, remained unchanged (
P
=0.35 and
P
=0.29, respectively). Hyperlipidemia, by clinical criteria, declined over time (
P
<0.01), but increased by self‐report (
P
<0.01). Smoking declined (
P
<0.01), and obesity increased by both body mass index (
P
<0.01) and waist/height ratio (
P
<0.01).

Conclusions

The perception that young adult risk factors are increasing is consistent with increasing self‐reported risk factors. However, evidence does not suggest that clinical risk factor prevalence overall has increased in young adults.
Association Between Maternal Folic Acid Supplementation and Congenital Heart Defects in Offspring in Birth Cohorts From Denmark and Norway
Authors: Øyen Nina , Olsen Sjurdur F. , Basit Saima , Leirgul Elisabeth , Strøm Marin , Carstensen Lisbeth , Granström Charlotta , Tell Grethe S. , Magnus Per , Vollset Stein E. , Wohlfahrt Jan , Melbye Mads
Published: March 2019

Background

Evidence linking individual‐level maternal folic acid supplementation to offspring risk of congenital heart defects is lacking. We investigated whether folic acid supplementation in early pregnancy reduces offspring risk of heart defects in 2 large birth cohort studies.

Methods and Results

Women recruited in early pregnancy within the DNBC (Danish National Birth Cohort), 1996–2003, and MoBa (Norwegian Mother and Child Cohort Study), 2000–2009, were followed until delivery. Information on periconceptional intake of folic acid and other supplements was linked with information on heart defects from national registers. Among 197 123 births, we identified 2247 individuals with heart defects (114/10 000). Periconceptional (4 weeks before through 8 weeks after conception) use of folic acid plus other supplements (54.8%), folic acid only (12.2%), and non–folic acid supplements (5.0%) were compared with no supplement use (28.0%); the adjusted relative risks of heart defects were 0.99 (95% CI, 0.80–1.22), 1.08 (95%
CI
, 0.93–1.25), and 1.07 (95%
CI
, 0.97–1.19), respectively. For initiation of folic acid in the preconception period weeks −4 to −1 (33.7%) and the postconception periods 0 to 4 weeks (15.5%), 5 to 8 weeks (17.8%), and 9 to 12 weeks (4.6%), compared with no or late folic acid intake (29.1%), relative risks of heart defect were 1.11 (95%
CI
, 1.00–1.25), 1.09 (95%
CI
, 0.95–1.25), 0.98 (95%
CI
, 0.86–1.12), and 0.97 (95%
CI
, 0.78–1.20), respectively. Relative risks of severe defects, conotruncal defects, and septal defects showed similar results.

Conclusions

Folic acid was not associated with offspring risk of heart defects, including severe defects, conotruncal defects, or septal defects.
Revised description, systematic affinities, and biogeographic considerations of the Fijian endemic Aristolochia vitiensis (Aristolochiaceae)
Authors: GONZÁLEZ FAVIO , PABÓN-MORA NATALIA
Published: March 2019

The poorly known Aristolochia vitiensis is one of the few species of the genus that inhabits SW Pacific Islands. Here we revisit and complement the description of this Fijian endemic. Our study indicates that the species belongs to the subgenus Aristolochia sect. Diplolobus subsect. Euaristolochia. The trifid apex of the perianth is unique among the species of the subgenus Aristolochia and it indicates the presence of three distinct perianth organs, a trait that very rarely can be observed in anthetic flowers.
A review of the Caribbean hamlets (Serranidae, Hypoplectrus) with description of two new species
Authors: LOBEL PHILLIP S.
Published: March 2019

Thirteen species of the Western Atlantic genus Hypoplectrus (Serranidae) are currently recognized, two of which are described as new. Hypoplectrus maya n. sp. (Maya hamlet) is restricted to the coastal lagoon of the Meso-American Barrier Reef system in Belize. It is a solid iridescent blue, lacks nose spots and lacks black margins on fins. Hypoplectrus randallorum n. sp. (Tan hamlet) is found widely in the central and western Caribbean. Its color varies from light brown to tan and it has spots on the nose, at the base of the pectoral fin and occasionally on the upper part of the caudal peduncle. All identified species of Hypoplectrus are illustrated in live coloration along with examples of color variations in H. nigricans and H. unicolor. A historical review of Hypoplectrus is included with a discussion of issues concerning their taxonomy.
New taxonomic status and new replacement name for Macednus Emeljanov, 1962 (Hemiptera: Cicadellidae: Deltocephalinae)
Authors: XING JI-CHUN , LI ZI-ZHONG
Published: March 2019

Emeljanov (1962) established the leafhopper genus Macednus with the type species Macednus marginatus Emeljanov, 1962 from USSR (Maritime Territory). Later, Vilbaste (1967) placed Macednus Emeljanov, 1962 as a junior subjective synonym of Albicostella Ishihara, 1953. Albicostella has Thamnotettix albicosta Matsumura,1914 as its type species. Oman, et al. (1990) followed Vilbaste (1967) in treating Macednus as a junior synonym of Albicostella and included the genus in the tribe Athysanini of subfamily Deltocephalinae (Hemiptera: Cicadellidae). Later, Hamilton (1975) reinstated Macednus as a separate genus.
Redescription of Holarctias rufinularia (Staudinger, 1901), with notes on the taxonomy and relationships of Holarctias Prout, 1913 (Lepidoptera: Geometridae: Sterrhinae)
Authors: BELJAEV EVGENY A.
Published: March 2019

The types of a little known species Holarctias rufinularia (Staudinger, 1901) are illustrated for the first time and redescribed, and misidentifications of this species are corrected. Photos of the types of Acidalia rufularia Eversmann, 1851, and Acidalia rufociliaria Bremer, 1864, are published for the first time. Species composition, diagnosis and relationships of the genus Holarctias Prout, 1913 are discussed.
Stella Vladimirovna Vassilenko (1936−2011): in memoriam
Authors: PETRYASHOV VICTOR V. , DANELIYA MIKHAIL E. , CHERNYSHEV ALEXEY V.
Published: March 2019

Stella Vladimirovna Vassilenko—Russian zoologist, carcinologist, biogeographer, prominent specialist in amphipods, particularly skeleton shrimps (Caprellida), hooded shrimps (Cumacea) and crabs (Decapoda: Brachyura) of cold and moderate waters of the World Ocean; author and co-author of 6 families and subfamilies, several genera and many crustacean species.
Phrynobatrachus pintoi Hillers, Zimkus & Rödel, 2008 (Amphibia: Anura: Phrynobatrachidae): additional morphological and distribution data
Authors: RÖDEL MARK-OLIVER , DOUMBIA JOSEPH , KOUAMÉ N’GORAN GERMAIN , SANDBERGER LAURA
Published: March 2019

Currently 14 Phrynobatrachus species are known to occur in Guinea (Frétey 2008; Frost 2011). The recently discovered species, Phrynobatrachus pintoi Hillers, Zimkus and Rödel, 2008, was described from the Boké region, north-western Guinea (Hillers et al. 2008). Although this description comprised distinct morphological and genetic characters, it was only based on one, subadult specimen. Further data on the species morphological variability, its biology and distribution, are thus needed. During a survey from 28 September to 20 October 2010 in the Télimélé region, north-western Guinea, we recorded this species at three additional localities. The respective data are presented herein.
The names of decapod and stomatopod Crustacea from Tahiti, French Polynesia, established by Anthony Curtiss in 1938 and 1944
Authors: NG PETER K. L. , ELDREDGE LUCIUS G. , EVENHUIS NEAL L.
Published: March 2019

In two poorly known books published in 1938 and 1944, Anthony Curtiss described 21 species of decapod and stomatopod crustaceans from Tahiti. Of these, 20 are found to be junior synonyms of well-known shallow-water species. One species, however, Cancer raihoae Curtiss, 1938, is a possible senior synonym of Portunus hawaiiensis Stephenson, 1968 (Portunidae).
New and little known species of the genus Martiodrilus Michaelsen, 1936 from French Guiana (Oligochaeta, Glossoscolecidae)
Authors: CSUZDI CSABA , PAVLÍČEK TOMÁŠ
Published: March 2019

The genus Martiodrilus is native to South America, with two species, Martiodrilus tenkatei (Horst, 1887) and Martiodrilus duodenarius (Michaelsen, 1918), previously recorded from French Guiana. In this paper we verified the presence of both species in French Guiana and provided a new record, Martiodrilus (Botaria) helleri (Michaelsen, 1918). Furthermore, two new species were described: Martiodrilus (Botaria) dewynteri sp. nov. and Martiodrilus (Maipure) gaucheri sp. nov. The subgeneric division of the genus Martiodrilus is briefly discussed.
Six unusual Cladotanytarsus Kieffer: towards a systematics of the genus and resurrection of Lenziella Kieffer (Diptera: Chironomidae: Tanytarsini)
Authors: GIŁKA WOJCIECH
Published: March 2019

Following the inclusion of Lenziella Kieffer, 1922 into Cladotanytarsus Kieffer, 1921, the genus consists of two subgenera: Cladotanytarsus s. str. (type species: C. pallidus Kieffer, 1922) and Lenziella Kieffer, 1922 (type species: L. bicornuta Kieffer, 1922). Six species of the subgenus Lenziella known from the Holarctic region are diagnosed and reviewed. Lectotype of Cladotanytarsus wexionensis Brundin, 1947 is designated, and the name is treated as a synonym of Cladotanytarsus (L.) bicornutus Kieffer, 1922 in a new combination. Cladotanytarsus (L.) amandus Hirvenoja, 1962 (male) and C. (L.) crusculus (Sæther, 1971) (male, female, pupa) are redescribed. Cladotanytarsus (L.) latissimus sp. nov. (male, female, pupa), C. (L.) piniger sp. nov. (male), and C. (L.) subletteorum sp. nov. (male, female, pupa) are described. Keys to adults and pupae of Lenziella as well as data on biology of the species are presented.
Mberu, a new neurigonine genus from southeastern Brazil (Diptera: Dolichopodidae)
Authors: CAPELLARI RENATO SOARES , DE SOUZA AMORIM DALTON
Published: March 2019

A new genus of Dolichopodidae with modified wing venation both in males and females is described from the Atlantic Forest in southeastern Brazil, Mberu pepocatu gen. nov. et sp. nov. The species is described and illustrated in detail, including the male and female terminalia. The systematic position of Mberu is considered and the genus is assigned to the tribe Coeloglutini of Neurigoninae, possibly closely related to Coeloglutus Aldrich and Neotonnoiria Robinson.
Disparia primavera, a new species of Notodontidae (Lepidoptera) from China
Authors: SALDAITIS AIDAS , IVINSKIS POVILAS , RIMSAITE JOLANTA
Published: March 2019

The genus Disparia Nagano, 1916 contains 15 species from the Palaearctic which are best separated by male genitalia (see Schintlmeister, 2008). Disparia are distributed throughout Southeast Asia, especially China (Kobayashi, Kishida & Wang, 2003, 2005; Schintlmeister & Fang 2001; Schintlmeister 2007, 2008; Wu & Fang 2003). A. Floriani collected an unknown Disparia in April 2011 at light in Sichuan Province, China, and we describe it herein as new.
A redefinition of the Curculionini of the world, with a key to subtribes and genera, and two new genera: Pseudoculio and Megaoculis (Coleoptera: Curculionidae: Curculioninae)
Authors: PELSUE, JR. FRANK W. , O’BRIEN CHARLES W.
Published: March 2019

A key to the subtribes and genera of the Curculionini is presented, and two new genera are described: Pseudoculio with Curculio (Balaninus) discreticoxis Marshall, as its type species (new combination); and Megaoculis with Megaoculis egeri, new species, as its type species. Keys to the species of the new genera are provided. Photos of habitus are included for most taxa and of genitalia of the new species. The following taxa are described as new in this paper: subtribes Archariina, new subtribe, Erganiina, new subtribe, and Labaninina, new subtribe; and new species: Pseudoculio barclayi, new species, P. confusicoxis , new species, P. crinitus, new species, P. promissus, new species, P. spiesi, new species, and P. vittatus, new species. Curculio guyanensis Rheinheimer is reassigned to the new genus Megaoculis, new combination. The following genera placed in Incertae sedis in Alonso-Zarazaga and Lyal are removed from the tribe: Allocionus Hustache, Balaninorhynchus Fairmaire, Bradyninus Fairmaire, Semicardius Hustache and Trichanthonomus Hustache.
Parasitic copepods of the family Taeniacanthidae (Crustacea) from triggerfishes (Teleostei, Balistidae) and filefishes (Teleostei, Monacanthidae) collected in the Indo-West Pacific region, with descriptions of two new species of Taeniacanthus Sumpf, 1871
Authors: TANG DANNY , UYENO DAISUKE , NAGASAWA KAZUYA
Published: March 2019

Two new species of Taeniacanthus Sumpf, 1871 (Copepoda, Taeniacanthidae) are described from filefishes (Monacanthidae) caught in the Indo-West Pacific region: T. brayae n. sp. from Pervagor melanocephalus (Bleeker) collected from five localities within the Central Indo-Pacific realm and T. mcgroutheri n. sp. from Monacanthus chinensis (Osbeck) and Paramonacanthus choirocephalus (Bleeker) caught off the Australian coast. Taeniacanthus brayae n. sp. and T. mcgroutheri n. sp. are distinguished from their congeners by the presence of an elongate terminal endopodal segment of the antenna, a spinulated terminal process and one seta on the maxillary basis, six elements on the terminal exopodal segment of legs 2–4 and an armature of II, I, 2 and II, I, 1 on the terminal endopodal segments of legs 2 and 3, respectively. Taeniacanthus brayae n. sp. can be readily distinguished from T. mcgroutheri n. sp. by having one row (rather than multiple rows) of spinules on the large pectinate process of the antenna, four setae (rather than three) on the maxillule and a 3-segmented (rather than 2-segmented) endopod on legs 2–4. New host and/or locality records for the taeniacanthids Cirracanthus monacanthi (Yamaguti, 1939), C. spinosus Dojiri & Cressey, 1987, Nudisodalis acicula Dojiri & Cressey, 1987 and Taeniacanthus aluteri (Avdeev, 1977) parasitic on triggerfishes and filefishes, as well as supplementary morphological information for the females and the first descriptions of the males of C. monacanthi, C. spinosus and N. acicula, are also included.
Prodorylaimus filamentus sp. n. and Eutobrilus longicaudatoides sp. n. (Nematoda) from Lake Baikal, Russia
Authors: GAGARIN VLADIMIR G. , NAUMOVA TATYANA V.
Published: March 2019

Two nematode species found in Lake Baikal, Russia are described. Prodorylaimus filamentus sp. n. is morphologically close to P. longicaudatoides Altherr, 1968 and P. kralli Tsalolikhin, 1975. The new species can be separated from P. longicaudatoides by the longer body (L = 4.89–6.06 mm versus L = 2.0–3.5 mm), relatively longer tail (c′ = 18.4–25.0 versus c′ = 14–18), longer odontostyle (60–65 µm long versus 32–37 µm long), presence of double and wide guiding ring and longer spicules (89–90 µm long versus 70–78 µm long). It can also be separated from P. kralli by the longer tail (c = 4.3– 6.0, c’ = 18.4–25.0 versus c = 7.0–8.0, c′ = 11–16), lower “vulva-anus to tail length” ratio (1.6–2.2 versus 2.5–3.0), shorter odontostyle (60–65 µm long versus 75–80 µm long). Eutobrilus longicaudatoides sp. n. is closely related to E. anguiculus Tsalolikhin, 1977, but is clearly distinct in the shorter outer labial setae (9–10 µm long or 45–52% of the labial region diameter versus 15–20 µm long or 50–60 % of the labial region diameter), longer tail (males, c = 5.3–5.5, c’ = 11.4–11.8, females, c = 4.5–6.3, c′ = 15.1–16.7, versus males, c = 7.4–10.4, c′ = 8–9, females, c = 5.0–7.7, c′ = 10–12), smaller number of supplements (5 versus 6) and shorter spicules (47–53 µm long versus 66–68 µm long).
The genus Guernea Chevreux, 1887 from Korean waters (Crustacea: Amphipoda: Dexaminidae)
Authors: KIM YOUNG-HYO , HENDRYCKS ED A. , LEE KYUNG-SOOK
Published: March 2019

Four species of the dexaminid genus Guernea are reported from Korean coastal waters. This is the first record of the genus Guernea from Korea. Two of the species are identified as new species; Guernea jejuensis and Guernea namhaensis. The remaining two Japanese species Guernea ezoensis Ishimaru, 1987 and Guernea nullispina Hirayama, 1985 are new records for Korean waters. The species are fully illustrated and detailed comparisons are made with related species. A key to north Pacific Guernea species is also provided.
An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens
Authors: CESARI MICHELE , GIOVANNINI ILARIA , BERTOLANI ROBERTO , REBECCHI LORENA
Published: March 2019

We have in recent papers revealed that an integrative taxonomy approach helps to solve taxonomic problems in tardigrades. However, whole tardigrades are required for DNA work, which leaves no hologenophore voucher specimens with adult morphology. Using a novel methodology for the Tardigrada, we introduce the practice of collecting high quality maximum magnification light microscopy images of recently thawed animals to act as hologenophore voucher specimens of animals later used for DNA barcode sequencing. Within the framework of a DNA barcoding project on tardigrades, we collected a moss sample from the type locality of Macrobiotus terminalis Bertolani & Rebecchi, 1993 (Castelsantangelo, Central Apennines, Italy), a species of the “Macrobiotus hufelandi group”. Within the moss sample we found several animals and eggs with a morphology that corresponded to the original description of M. terminalis, while others were attributable to Macrobiotus macrocalix Bertolani & Rebecchi, 1993. In this study, molecular (cox1 mtDNA) analyses demonstrated no intraspecific variability in M. terminalis from the type locality but very large interspecific differences when compared with M. macrocalix and GenBank data for other species within the M. “hufelandi group”. There was also a large difference between our M. terminalis sequences and the GenBank data of a specimen attributed to the same species. The GenBank sequence originated from a population in the Northern Apennines, whose morphology appeared to be like that of the specimens of the locus typicus. This confirmed the importance in utilising material from the type locality for linking molecular data to the species’ morphological characters. Our paper underlines the importance of an integrative taxonomy in species diagnoses and demonstrates a scenario where morphological observations alone are not always sufficient. Lastly, this work adds reliable information to the sequence reference library that provides a useful building block for further studies on similar and related tardigrade taxa.
The spider Micrathena shealsi Chickering, 1960 (Araneae, Araneidae): description of the male, with new data on its geographic distribution
Authors: ARGAÑARAZ CARINA I. , RUBIO GONZALO D.
Published: March 2019

The male of Micrathena shealsi Chickering, 1960 is described and illustrated for the first time. New geographic records of this species are provided, revealing that M. shealsi inhabits the higher altitudinal belts (1000–3000 m) of the Yungas ecoregion (mountain forests and rainforests from Argentina). Male specimens were observed in the same web of females, in most cases on the upper periphery hanging by a silk line.
Circumscription, diagnosis and description of a subfamily of Australo-Papuan robins
Authors: LOYNES KATE , JOSEPH LEO , KEOGH J. SCOTT
Published: March 2019

The family-group name Microecinae Loynes, Joseph and Keogh, 2009 was erected by Loynes et al. (2009) following their phylogenetic analysis of the Australo-Papuan robins (Aves: Passeriformes: Petroicidae). Loynes et al. (2009) assigned to Microecinae one genus Microeca Gould, 1840, which as conventionally construed (see Boles 2007) comprises six species (M. flavigaster, M. fascinans, M. griseoceps, M. hemixantha, M. flavovirescens, M. papuana) found in Australia, New Guinea and some surrounding islands (see Schodde and Mason 1999, Boles 2007 for details). Loynes et al. (2009) were able to include only the first three of those six species in their analysis. They did, however, transfer a further species from Eopsaltria to Microeca, which was thus recognized as M. flaviventris.
New Lepidocyrtus Bourlet, 1839 taxa from Greece (Collembola: Entomobryidae)
Authors: MATEOS EDUARDO
Published: March 2019

Published data on genus Lepidocyrtus from Greece are scarce, being L. ligonorum the most common species in the region. In this paper the analysis of several populations of Lepidocyrtus from four Greek islands has allowed to describe the new species L. juliae sp. nov. and L. barbulus sp. nov. These new taxa were previously described as a color form and chaetotaxic variety of L. lignorum respectively in Greece. Along with other seven European species, the two new described taxa form the "Lepidocyrtus lignorum group", defined by the dorsal macrochaetae formula R 0 R 1 R 2 /00/0101+3 (with or without cephalic macrochaeta So). An identification key has been developed for differentiating all species of this group. With the new described taxa the number of Lepidocyrtus species present in Greece increases to five, and the total European Lepidocyrtus species increases to 29.
Cave millipeds of the United States. X. New species and records of the genus Pseudotremia Cope. 2. Species from Virginia, USA (Diplopoda, Chordeumatida, Cleidogonidae)
Authors: SHEAR WILLIAM A.
Published: March 2019

The milliped genus Pseudotremia Cope 1869 as it occurs in Virginia, USA, is reviewed, with new records and species, mostly from caves. Seventeen new species, Pseudotremia loomisi, P. contorta, P. cerberus, P. pomarium, P. orndorffi, P. peponocranium, P. glaber, P. fergusoni, P. jaculohamatum, P. fremens, P. hubbardi, P. ryensis, P. piscator, P. culveri, P. salfodina, P. johnholsingeri and P. inexpectata are described, for a total Virginia fauna of 25 species. Additional species are predicted to occur. New locality records are given for seven previously described species, Pseudotremia alecto Shear 1972, P. hobbsi Hoffman 1950, P. sublevis Loomis 1944, P. tuberculata Loomis 1939, P. momus Shear 1972, P. nodosa Loomis 1939, and P. valga Loomis 1943. Taxonomic characters useful in delimiting species of Pseudotremia are discussed.
New species of Acasta and Balanus (Balanoidea: Acastinae, Balaninae) from the Early Miocene (Burdigalian) of the Aquitaine Basin, France
Authors: CARRIOL RENÉ-PIERRE , CAHUZAC BRUNO , LESPORT JEAN-FRANÇOIS
Published: March 2019

Two new species of spiny barnacles (Balanoidea: Acastinae, Balaninae) are described from the lower Burdigalian (Early Miocene) of the Aquitaine Basin, southwest France. The first, Acasta martillacensis sp. nov., from Martillac, is characterised by a shell with internal longitudinal ribs, a cellular space between the sheath and wall, a carinal sheath which adheres to the wall in the centre of its basal edge, a cup-shaped basis and a scutum with radial striae externally and a less than prominent adductor ridge internally. The second, Balanus aculeatus sp. nov., from Saucats (Le Péloua), has paries exteriorly with strong, tubular spines. An overview of outcrops of Burdigalian strata in the area, within a palaeogeographic context, is added.
Monograph of Nylanderia (Hymenoptera: Formicidae) of the World: An introduction to the systematics and biology of the genus
Authors: LAPOLLA JOHN S. , BRADY SEÁN G. , SHATTUCK STEVEN O.
Published: March 2019

This paper serves as an introduction to a world monographic series addressing the species-level taxonomy of the ant genus Nylanderia. This series will consist of several regionally based taxonomic revisions. The systematics and biology of Nylanderia are discussed in a global context, and a diagnosis of the genus is given. Several morphological features, which are considered putative synapomorphies for the genus, are provided. Morphological descriptions of all three castes (workers, queens, and males) are provided and discussed.
Monograph of Nylanderia (Hymenoptera: Formicidae) of the World, Part I: Nylanderia in the Afrotropics
Authors: LAPOLLA JOHN S. , HAWKES PETER G. , FISHER BRIAN L.
Published: March 2019

The taxonomy of the Afrotropical Nylanderia fauna is revised for the first time. Fourteen native species are revealed, of which eight are described as new: N. boltoni LaPolla and Fisher, N. brevisetula LaPolla and Fisher, N. impolita LaPolla and Fisher, N. luteafra LaPolla and Fisher, N. scintilla LaPolla and Fisher, N. silvula LaPolla and Fisher, N. umbella LaPolla and Fisher, and N. usambarica LaPolla, Hawkes and Fisher. Two species, N. jaegerskioeldi and N. natalensis, have workers that are indistinguishable from each other, and males are the only reliable way to separate these two species. Three non-native Nylanderia species are thought to have been introduced to Africa: N. bourbonica, N. vaga, and N. vividula. An identification key to the worker caste is provided.
On some linyphiid spiders from Taiwan (Araneae: Linyphiidae)
Authors: TANASEVITCH ANDREI V.
Published: March 2019

Seven linyphiid species are recorded from Taiwan, three of which are new: Maculoncus orientalis n. sp., Oreonetides taiwanus n. sp. and Tapinocyba formosa n. sp. Three species, Micrargus herbigradus (Blackwall, 1854), Oia imadatei (Oi, 1964) and Solenysa longqiensis Li & Song, 1992, are new to the Taiwanese fauna. Based on the new data, the linyphiid fauna of Taiwan is known to contain 20 linyphiid species.
Geophilus arenarius, a long-misunderstood species in the still unresolved carpophagus species-complex (Chilopoda: Geophilidae)
Authors: BONATO LUCIO , MINELLI ALESSANDRO
Published: March 2019

Geophilus arenarius Meinert (Chilopoda: Geophilidae), a neglected nominal species from north-western Africa of uncertain identity, is here described in detail and its taxonomic position assessed. G. arenarius is actually a morphologically distinct species belonging to a widespread Western Palaearctic species-complex whose internal taxonomy is still largely unresolved, to the exclusion of the two British species G. carpophagus and G. easoni. G. arenarius differs from both other species mainly by lacking a transverse suture on the head, lacking peculiar integumental features (carpophagus-structures) along the trunk, and having relatively stouter antennae and forcipular coxosternite.
Eremias papenfussi sp. nov., a new lacertid lizard (Sauria: Lacertidae) from Tehran Province, Iran
Authors: MOZAFFARI OMID , AHMADZADEH FARAHAM , PARHAM JAMES F.
Published: March 2019

We describe a new species of Eremias lacertid from the Alborz Mountain range in northern Iran (Tehran Province). Eremias papenfussi n. sp. is part of the Eremias subgenus (or morphotype) by virtue of lacking lateral fringes on the fourth toe and color pattern. It can be further differentiated from previously described species assigned to this morphotype by the absence of distinctly keeled upper caudal scales, gular scales that do not extend to the second inframaxiallary scales, color pattern, and scale counts. Eremias papenfussi is found on rocky mountain slopes of the Alborz where it is presumed to have a much broader distribution than demonstrated by the available specimens. Of the 15 species of Eremias known from Iran, E. papenfussi is the fifth species known to inhabit rocky mountain slopes along with E. strauchi, E. lalezharica, E. montanus and E. novo.
Speleonectes williamsi, a new species of Remipedia (Crustacea) from the Bahamas
Authors: HARTKE TAMARA R. , KOENEMANN STEFAN , YAGER JILL
Published: March 2019

We describe a new species of the genus Speleonectes (Crustacea, Remipedia, Nectiopoda) from an anchialine cave on Grand Bahama Island in the northern Bahamas. Speleonectes williamsi n. sp. is morphologically highly similar to Speleonectes emersoni from the Dominican Republic. However, morphological differences between the two species were detected in dissected body parts, such as the setal patterns of the antennae and trunk limbs, the terminal claws of maxillae and maxillipeds, and the frontal filaments.
Phylogenetic analysis of Arsenura Duncan (Lepidoptera, Saturniidae, Arsenurinae) based on adult morphology
Authors: DE CAMARGO AMABÍLIO J. A. , MIELKE OLAF H. H. , CASAGRANDE MIRNA M.
Published: March 2019

Arsenurinae includes 10 Neotropical genera with 74 species. Arsenura Duncan, 1841 is represented by 30 species and 13 subspecies, making it the largest genus of the subfamily. The species of this group occur both in the Andean high altitude and at sea level, but present higher biodiversity at lower altitudes. Most of the immature remain unknown; however, it is known that some larvae feed on several plant families. There are no prior studies on the phylogenetic relationship between the species of the genus. The goal of this study was to establish the relationships between Arsenura species using cladistic analysis, based on adult morphological characters. Arsenura was recovered as a monophyletic group, with the following relationships between species: ((A. meander, A. pandora)((A. polyodonta(A. orbignyana, A. xanthopus))(A. rebeli((A. mossi(A. beebei, A. ciocolatina))(A. archianassa(A. armida(A. albopicta, A. delormei)))))((A. aspasia, A. biundulata)((A. sylla,A.thomsoni)(A. cymonia(A. ponderosa(A. batesii, A. drucei))))))).
Two new species of the genus Geocharis Ehlers, 1883 and new data on Anillina species from Portugal (Coleoptera: Carabidae)
Authors: SERRANO ARTUR R. M. , AGUIAR CARLOS A. S.
Published: March 2019

Two new species of the genus Geocharis Ehlers, 1883 (Carabidae: Trechinae, Anillini) are described (Geocharis barcorabelo n. sp. and Geocharis antheroi n. sp.) from the northern region of Portugal (Iberian Peninsula). This work provides diagnostic characters for these species and, in particular, for the structure of male genitalia. The affinities with other known species namely their putative closest relatives, as well as ecological and biogeographical comments are also included. A key for the identification of the males of the Geocharis species found north of the Tejo River in Portugal is also provided. New chorologist data on other endogean carabids from Portugal are also included.
Coarazuphium tapiaguassu (Coleoptera: Carabidae: Zuphiini), a new Brazilian troglobitic beetle, with ultrastructural analysis and ecological considerations
Authors: PELLEGRINI THAIS GIOVANNINI , FERREIRA RODRIGO LOPES
Published: March 2019

Coarazuphium tapiaguassu sp. n. was collected in caves SL 30, SL 31 and SL 35 located in Curionópolis (Pará, Brazil). C. tapiaguassu is readily differentiated by the absence of setae on the dorsal surface of the head close to the posterior margin, as the other species of the gens show one, two or tree pairs of setae. Taxa of Coarazuphium exhibit advanced troglobiomorphic characters in comparison to other Brazilian cave beetles. Increased extra-optic sensory structures, presence of particular sensilla, and sensory and gustatory receptors are characters not detected under routine microscopy and thus require ultrastructural methods for their study. Similar analyses are needed in other epigean Zuphiini species for a better interpretation of their functional meaning.
Strotihypera, a new Old World genus of the tribe Elaphriini (Lepidoptera, Noctuidae, Noctuinae)
Authors: KONONENKO VLADIMIR S. , HAN HUI-LIN
Published: March 2019

A new genus Strotihypera gen. n. (type-species Erastria flavipuncta Leech, 1889) is described in the tribe Elaphrriini, subfamily Noctuini from the Eastern Palaearctic. The new combination Strotihypera flavipuncta (Leech, 1889) comb. n. is introduced for Hyperstrotia flavipuncta (Leech, 1889) A review of the Old World genera Strotihypera, Elaphria and Galgula of the tribe Elaphriini is presented.
New and known species of the family Leuconidae(Cumacea, Peracarida) from Antarctic deep-sea basins
Authors: MÜHLENHARDT-SIEGEL UTE
Published: March 2019

The leuconid cumaceans of the Antarctic deep-sea basins are listed. Ten new leuconid species are described: eight species in the genus Leucon: L. (Crymoleucon) parantarcticus, L. (C.) weddellensis, L. (C.) longirhinus, L. (C.) grandidentatus, L. (Leucon) quattuormulierum, L. (L.) andeep, L. (Macrauloleucon) weigmanni and L. (M.) complexus. One new species is described for the genus Eudorella: E. flokkeri. The genus Bytholeucon was discovered for the first time in Antarctic waters with a new species, B. hartmannorum.