bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics and Antibacterial Activity of ZnO Nanoparticle Fortified Probiotic Yogurt
Authors: Erlita Sari Pipit , Abidin Zaenal , Arief Irma Isnafia , Budiman Cahyo
Published: January 2025

Zinc deficiency is a major health issue in developing nations, especially in Southeast and South Asia. To address this, food fortification using nanotechnology, such as ZnO nanoparticles deemed safe by the US FDA, can enhance daily zinc intake essential for growth and immune health. This research emphasizes on yoghurt fortification with various types: ZnO nanoparticle fortification and the addition of probiotic bacteria Lactiplantibacillus plantarum subsp. plantarum IIA-1A5 (Accession Number: OR473281) and aims to analyze the nutritional value, characteristics, total lactic acid bacteria (LAB) and antibacterial activity of probiotic yogurt fortified with ZnO nanoparticles. There were six levels of treatment designed in this study and each treatment was tested three times. The statistical test used in analyzing the nutritional value, characteristics, total LAB and antibacterial activity in this study is the Tukey test which is applied as a multiple range test while in organoleptic testing using the Kruskal-Wallis non-parametric test. The results showed that the fortification of yogurt with ZnO nanoparticles had significant interaction (p<0.05) on yogurt viscosity, LAB content, and color attributes in hedonic quality test. The antibacterial activity towards Staphylococcus aureus and Escherichia coli produced by six treatments were moderate, with inhibition zones ranging between 5 to 10 mm. This study concluded that the fortified yoghurt treatment met the Indonesian National Standard (SNI), demonstrating the potential of this approach in addressing zinc deficiency in at-risk populations. This research underscores the promise of nanotechnology in food fortification strategies to combat micronutrient deficiencies globally
Sperm Kinematics of Pesisir Bull Thawed at Different Temperatures and Times
Authors: Hendri Hendri , Jaswandi Jaswandi , Indriastuti Rhesti , Ananda Ananda
Published: January 2025

Pesisir bulls from West Sumatra, Indonesia, have the potential to be valuable meat producers due to their adaptability and unique characteristics, but reproductive challenges necessitate the use of artificial insemination (AI) technology. The success of AI relies on the quality of frozen semen, which is influenced by thawing conditions, making the evaluation of sperm kinematics through Computer-Assisted Sperm Analysis (CASA) crucial for a comprehensive assessment of sperm function. This research evaluated the kinematics of frozen semen spermatozoa from Pesisir bulls. Frozen semen samples (n = 20) were thawed at 37°C for 10, 20, and 30 s and at 25°C for 30, 45, and 60 s. Kinematic parameters were observed using a Computer-Assisted Sperm Analyzer (CASA), including total motility (M), progressive motility (PM), the velocity of the average path (VAP) (𝜇m/sec), the velocity of curvilinear (VCL) (𝜇m/sec), the velocity of straight line (VSL) (𝜇m/sec), straightness (STR) (%), linearity (LIN) (%), wobble (WOB) (%), amplitude of lateral head displacement (ALH) (𝜇m), and beat/cross-frequency (BCF) (Hz). Thawed frozen semen at a temperature of 37°C for 20 s significantly (p<0.05) increased M, PM, and ALH. Furthermore, thawed frozen semen at 37°C for all durations and at 25°C for 60 s showed better quality for VAP, VCL, and VSL (p<0.05). Meanwhile, thawed frozen semen at 25°C increased STR, LIN, and WOB. However, thawing temperature and duration had no significant effect on BCF. Correlation analysis conducted on semen thawed at a temperature of 37°C for 20 s found that VAP was correlated with VCL, VSL, and ALH, while semen thawed at a temperature of 25°C for 60 s found that VAP was correlated with VCL, VSL, STR, LIN, and ALH. Most kinematic parameters were significantly better in the thawing treatment at 37°C compared to 25°C, suggesting careful consideration
Physicochemical and Sensory Properties of Goat Meat Sausages Prepared With Different Levels of Goat Liver
Authors: Triyannanto Endy , Rusman Rusman , Cahyowati Meireni , Kayihura Joseph , Sitaresmi Pradita Iustitia
Published: January 2025

Anemia, a common issue among children and teenagers in Indonesia, is characterized by hemoglobin levels below normal. Iron is essential for producing hemoglobin in red blood cells, which helps prevent anemia. Innovation to provide a popular goat liver-based product that is convenient to consume and easily marketable underpins the development of this goat liver-enhanced sausage. The aim of this research was to determine the effect of partially substituting goat meat with different levels of goat liver on physicochemical and sensory properties of goat meat sausages. Sausages containing 100:0, 75:25, and 50:50 goat meat to goat liver ratios and goat meat (%, w/w) , and then added with other ingredients (tapioca flour, skim milk, garlic, pepper, coriander, salt, STPP, and ice water) were prepared and cooked for 30 minutes. The sausages were then analyzed for physical properties, including pH, texture and water holding capacity (WHC), iron content, and sensory quality. Data were analyzed using analysis of variance for a completely randomized design, and then if there were significant differences between means, they were tested using Duncan’s New Multiple Range Test. The results showed that substitution of goat liver significantly (p0.05). In conclusion, substituting up to 50% of goat meat with goat liver will improve the iron content of goat meat sausages with minimal impact on the sensory and proximate composition of the product.
Estrategias de poder en el clero urbano hispalense en los siglos XVIII y XIX. Caso de las familias Paulín, Pérez de Baños y de la Barrera
Authors: Regalado González-Serna Víctor Daniel
Published: January 2025

En el presente trabajo se analizan varias generaciones de miembros del cabildo catedral de Sevilla que pertenecieron a la familia Paulín-Pérez de Baños-De la Barrera. Estos apellidos formaron un mismo grupo familiar en Sevilla en los inicios del siglo XVIII y ha sido posible rastrearlo hasta finales del XIX en sus relaciones con el alto clero urbano. De esta manera, el objetivo de esta investigación es entender mejor las posibles estrategias de poder desarrolladas por grupos familiares en el Antiguo Régimen y comienzos del Nuevo, comprendiendo más profundamente los sistemas de colocación de parientes.
Del sambenito al hábito. Ascenso y transformación en la Córdoba moderna: Los Fernández Tejeiro de Cabra
Authors: Reverte Ruedas Jorge
Published: January 2025

En los últimos años, se ha producido un avance significativo en la superación de los estereotipos que han rodeado a este campo de estudio, gracias al esfuerzo y dedicación de algunos historiadores. En la actualidad, tenemos un entendimiento más preciso de una sociedad aparentemente estática, donde incluso los segmentos menos privilegiados, como los judeoconversos, lograron ascender hacia posiciones elevadas que teóricamente deberían haberles sido inaccesibles. Este trabajo elige examinar el caso paradigmático de una familia egabrense de origen cuestionable, los Fernández Tejeiro, quienes, en poco más de un siglo, pasaron de ser conversos notorios a ocupar roles destacados en la nobleza española.
El mayorazgo de Ayamonte: conflicto sucesorio entre el X duque de Béjar y la III marquesa de Villamanrique (1662-1676)
Authors: Melero Muñoz Isabel Mª
Published: January 2025

En 1498, Teresa de Zúñiga y Guzmán, hija del duque de Medina Sidonia, fundó el mayorazgo de Ayamonte vinculando las villas de Lepe, Ayamonte y la Redondela en favor de su hijo segundo, Francisco de Guzmán y Zúñiga, quién se intituló I marqués de Ayamonte. Más de un siglo después, en 1662, tuvo lugar un complejo pleito sucesorio por el mayorazgo protagonizado por las distintas ramas del linaje. Tras la muerte de la última poseedora, Brianda de Zúñiga y Guzmán, VI marquesa de Ayamonte, sin dejar sucesión, el vínculo vacante fue litigado por las líneas colaterales de la familia. Así, el litigio fue protagonizado por el X duque de Béjar y la III marquesa de Villamanrique, quiénes se enfrentaron en el proceso jurídico para conseguir el importante mayorazgo y marquesado de Ayamonte. En este trabajo, se analiza la fundación del vínculo, así como el complejo pleito sucesorio que tuvo lugar, en el que se trataron temas trascendentales como eran la incompatibilidad de los mayorazgos y las armas y apellidos del linaje.
Surface morphology, internal structure and nucleation mechanism of graphite inclusions in ductile cast iron.
Authors: Pokrovsky A. I.
Published: January 2025

The surface morphology and internal structure of graphite inclusions in ductile cast iron are investigated. Based on this, the theory of graphite nucleation on solid inclusions in the melt is confirmed. It is shown that the morphology of the outer surface of spherical graphite inclusions can vary from almost smooth to consisting of successively overlapping graphite flakes. It is suggested that, depending on the thermophysical conditions of crystallization, the growth of inclusions along one of the crystallographic planes prevails: [1010] for slow cooling (scaly surface morphology of the “cabbage head” type) and [0001] for faster cooling (smooth surface).

Metallographic studies of the internal structure of the spherical graphite inclusion has revealed its segmental‑layered structure with characteristic concentric wavy lines. Inside the inclusion, characteristic zones are distinguished: the center, sectors with characteristic junctions diverging from the center, and sometimes inclusions of siliceous ferrite are found. The polycrystalline structure of the inclusion with characteristic boundaries between graphite pyramids has been confirmed. The EPMA method revealed anomalies in the distribution of carbon, magnesium, silicon, sulfur, and oxygen concentrations in the graphite inclusion. In the center, content of the magnesium, sulfur, and oxygen is significantly increased. This confirms the theory of graphite nucleation on sulfides and oxides. In some cases, an increase in the oxygen content was observed on the outer part of the inclusion, which may be due to the displacement of oxides to the periphery during the growth of the graphite inclusion in the melt. The obtained results provide clarifi ations and supplementations to the theory of heterogeneous nucleation of spheroidal graphite particles in ductile cast iron, where the central part of the spheroid is a conglomeration of complex sulfides and oxides.
Analysis and forecasting of the impact of working conditions on occupational diseases in foundry workers
Authors: Lazarenkov A. M. , Ivanov I. A. , Sadokha M. A. , Novik A. A.
Published: January 2025

The article examines the impact of working conditions on employees in foundry production. The analysis of occupational diseases in foundries with different production profiles is presented. It is noted that the highest number of occupational diseases occurs in the cleaning and grinding, molding, and melting‑pouring sections of the foundry. The highest number of cases across various types of production is found among grinding operators, molders, furnace operators, pourers, and repair mechanics.
Technological Transformation: Growing Opportunities and Legal Response to Emerging Risks
Authors: Begishev I. R. , Zharova A. K. , Zaloilo M. V. , Filipova I. A. , Shutova A. A.
Published: January 2025

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Sociological and Criminological Research of Victimization Issues: Preliminary Stage and New Sphere of Cybercrime Categorization
Authors: Auwal A. M. , Lazarus S.
Published: January 2025

Objective: to identify the main issues of victimization as a result of cybercrime growth in the world in general and in Nigerian society in particular from the standpoint of sociological approaches, using a Tripartite Cybercrime Framework (TCF), which comprises geopolitical, psychosocial and socioeconomic categories of cybercrime.

Methods: the methodology is based on the sociological research method. The data collection included the distribution of a questionnaire among 896 participants from the academic environment, including students and university staff, and the analysis of the responses. The presented data were analyzed using descriptive statistics, with special attention to the issues of gender inequality, socio-economic factors, the impact of educational level on vulnerability to online fraud and victimization as a result of cybercrime through the prism of the ideal victim concept and the socio-economic gap between North and South.

Results: the article presents an analysis of the Tripartite Cybercrime Framework. The survey showed that 65.20% of the participants had been victims of cybercrime. There were more men among the victims (64.69%). The authors found patterns in the distribution of cybercrimes. All cybercrimes against the respondent were socio-economic ones, which underlines the high frequency of cybercrime and the relevance of countering it in Nigerian society. Special attention was paid to the issues of gender inequality, socio-economic factors, and the impact of education on vulnerability to cybercrime. The article considers from the viewpoint of the ideal victim concept. The study results provide an idea of the prevalence and distribution of specific types of cybercrime in the socio-economic category among the studied population.

Scientific novelty: For the first time, the study uses the Tripartite Cybercrime Framework (TCF) to study victimization as a result of cybercrime in Nigerian society. The research novelty is also due to the fact that the conceptual foundations of countering cybercrime that have developed in the global North are not fully applicable in Nigeria.

Practical significance: the results obtained demonstrate the need to apply carefully calibrated gender-based, inclusive and contextual approaches to the development of a national legal policy to combat cybercrime. The results can be used to justify the law-making decisions which are being developed in the field of preventing and countering manifestations of cybercrime, as well as to form the basis for legal measures to protect cybercrime victims.
Legal Aspects of Personal Data Protection and the Issues of Competition in Digital Marketplaces
Authors: Kolawole A.
Published: January 2025

Objective: to develop approaches and proposals to improve the legal mechanisms for the personal data protection in the context of the evolving digital markets and the growing digital competition.

Methods: the article is prepared based on formal legal and comparative legal research methods.

Results: the unique features of digital markets are shown, which must be taken into account to achieve the goals of antimonopoly legislation. It is marked that the fundamental elements of the digital market include the big data concept and big data analytics, which, based on digital platforms, are capable of producing many direct and indirect network effects. The latter require understanding for an effective antitrust response and the application of appropriate legislation. The author proves that the growth of digital platforms as a business model and vital infrastructure of the digital economy should be viewed as a factor in improving legal regulation of relations in the sphere of data protection and confidentiality. The paper identifies the potential of digital platforms for assessing the current market power and the impact of competition on limiting this power. The data are considered as an integral component of the overall competitive market landscape. The current European Union regulation in the field of digital platforms and personal data protection is analyzed. The author identifies difficulties associated with the creation and application of effective regulations governing the activities of digital platforms. The article proves that antimonopoly authorities need to change approaches to analytics in order to take into account the distinctive features of digital platforms. It is noted that such changes may require legislative reforms and revision of procedures to match the rapid development of these markets and ensure that any potentially anticompetitive behavior is thoroughly investigated.

Scientific novelty: the research contributes to the development of approaches to determining indicators of ensuring the personal data confidentiality under the digitalization of markets and to evaluating the effectiveness of antimonopoly legislation and its application in a new competitive environment.

Practical significance: the results obtained can be used as a basis for improving antimonopoly and personal data protection legislation, as well as legal regulation of digital platforms in general.
The kinase domain of TRPM7 interacts with PAK1 and regulates pancreatic cancer cell epithelial-to-mesenchymal transition
Authors: Gautier Mathieu , Auwercx Julie , NEVE Bernadette , VANLAEYS Alison , Bourrin-Reynard Ingrid , SOUDI Mouloud , Brassart-pasco Sylvie , HAGUE Frédéric , Guénin Stéphanie , DUCHENE Belinda , Gutierrez Laurent , Destaing Olivier , DHENNIN-DUTHILLE Isabelle , SEUNINGEN Isabelle VAN , Jonckheere Nicolas
Published: January 2025

Pancreatic ductal adenocarcinoma (PDAC) is the main and the deadliest form of pancreatic cancer. This is a major problem of public health since it will become the second leading cause of death by cancer in the next few years, mainly due to the lack of efficient therapies. Transient Receptor Potential Cation Channel Subfamily M Member 7 (TRPM7) protein, a cation channel fused with a serine/threonine kinase domain is overexpressed in PDAC and associated with a low survival. In this work, we aim to study the role of kinase domain on pancreatic cell fates by using a model of kinase domain deletion by CRISPR-Cas9. PANC-1 and MIA PaCa-2 PDAC cell lines were used and kinase domain was deleted by CRISPR-Cas9 strategy. Kinase domain deletion (ΔK) was validated by RT-qPCR and western-blots. The effect of kinase domain deletion on channel function was studied by patch-clamp and Mn2+-quenching. The cell phenotype was studied by MTT and cell migration/invasion assays. Finally, the role of kinase domain was studied in vivo in xenografted mice. Here we show that TRPM7 kinase domain is required to maintain a mesenchymal phenotype in PDAC cells. We also demonstrated that TRPM7 and PAK1 interact in the same protein complexes. Moreover, TRPM7 kinase domain is required for carcinogenesis and cancer cell dissemination in vivo. Intriguingly, the role of TRPM7 kinase is cell specific and may depend on the KRAS oncogene mutation status. In conclusion, TRPM7 kinase domain is required to maintain a mesenchymal and aggressive phenotype in PDAC cells, and it could be a promising target against PDAC.
Humans have substantially extended fire seasons in all biomes on Earth
Authors: Ellis Todd , Bowman David , Williamson Grant
Published: January 2025

The conjunction of lightning ignitions and dry vegetation has shaped fire regimes throughout geological time. These natural fire regimes have in turn influenced the adaptation of biotas globally. Anthropogenic fire use, however, radically transformed worldwide fire regimes by extending seasons to the limits bounded by periods of high fuel moisture. Conversely, active fire suppression has typically limited the occurrence and extent of lightning ignited fires – particularly where population density is higher. Disaggregating contemporary human- and lightning-caused seasonal fire patterns globally can shed light on the magnitude of the anthropogenic change to fire regimes among biomes. Using global datasets of flammability moisture thresholds and fuel moisture we define the global distribution of fire seasons. We then use a global record of lightning strike density to disaggregate the fire season into mixed (i.e., lightning present) and anthropogenic ignitions periods, before describing the respective importance of these periods amongst biomes. We assess these the breadth of these ignitions periods against a daily satellite burned area record (2001–2023) and contextualise the occurrence of areas burned within mixed and anthropogenic ignitions periods against vegetation productivity and human population density using the established productivity-fire activity relationship. Collectively, we show that current anthropogenic influences have transformed worldwide fire regimes by substantially lengthening fire seasons, irrespective of local land use and fire suppression practices, population density, lightning occurrence, and biome type.
Linoleic acid promotes TF expression through PPAR-α, which leads to tumor progression in primary pulmonary lymphoepithelioma-like carcinoma
Authors: Bao Hejing , Zhang Jiani , Chen Zhuoyan , Wang Yuhuan , Wang Zhe , Chen Zhiting , Jiang Ting , Zhang Baishen , Zeng Wen , Bao Hehong , Ma Shudong
Published: January 2025

Primary pulmonary lymphoepithelioma-like carcinoma (pLELC) is a relatively uncommon variant of primary non-small cell lung cancer, and its etiology is still largely unexplored. Objective: The aim of this study is to investigate the underlying mechanisms and potential therapeutic targets associated with pLELC. The patients diagnosed with advanced pLELC were retrospectively collected and subjected to proteomics and metabonomics analysis. Finally, a patient-derived xenograft (PDX) model of pLELC xenograft was constructed for validation. The results of the data-independent acquisition(DIA) quantitative analysis revealed that the expression of tissue factor (TF) protein was found to be upregulated in pLELC. Furthermore, it was observed that TF protein played a role in iron death, hypoxia-inducible factor-1 (HIF-1) signalling pathway, and leukocyte transendothelial migration. Untargeted metabonomics analysis revealed the presence of major metabolites, namely linoleic acid (LA), free fatty acid (16:0), and histidine. LA has been found to contribute to the progression of tumors by promoting the infiltration of M2 tumor-associated macrophages and inhibiting the infiltration of natural killer(NK) cells. However, this effect can be reversed by the TF inhibitor Tisotumab. LA enhances the expression of TF through peroxisome proliferator-activated receptor (PPAR)-α, and the malignancy caused by LA can be counteracted by TF inhibitors.The findings of this study suggest that LA has the ability to alter the tumor microenvironment in pLELC by upregulating TF expression through PPAR-α. These results indicate that TF could potentially serve as a therapeutic target for pLELC.
Evaluating a Customised Large Language Model (DELSTAR) and its Ability to Address Medication-Related Questions Associated with Delirium: A Quantitative Exploratory Study
Authors: Spagl Katharina Teresa , Watson Edward William , Jatowt Adam , Weidmann Anita Elaine
Published: January 2025

Background: A customised large language model (LLM) could serve as a next-generation clinical pharmacy research assistant to prevent medication-associated delirium. Comprehensive evaluation strategies are still missing.
Aim: This quantitative exploratory study aims to develop an approach to comprehensively assess the domain-specific customised delirium LLM (DELSTAR) ability, quality and performance to accurately address complex clinical and practice research questions on delirium that typically require extensive literature searches and meta-analyses.
Method: DELSTAR, focused on delirium-associated medications, was implemented as a 'Custom GPT' for quality assessment and as a Python-based software pipeline for performance testing on closed and leading open models. Quality metrics included statement accuracy and data credibility; performance metrics covered F1-Score, sensitivity/specificity, precision, AUC, and AUC-ROC curves.
Results: DELSTAR demonstrated more precise information compared to information retrieved by traditional systematic literature reviews (SLRs) (p<0.05) and accessed Application Programmer Interfaces (API), private databases, and high-quality sources despite mainly relying on less reliable internet sources. GPT-3.5 and GPT-4o emerged as the most reliable foundation models. In Dataset 2, GPT-4o (F1-Score: 0.687) and Llama3-70b (F1-Score: 0.655) performed best, while in Dataset 3, GPT-3.5 (F1-Score: 0.708) and GPT-4o (F1-Score: 0.665) led. None consistently met desired threshold values across all metrics.
Conclusion: DELSTAR demonstrated potential as a clinical pharmacy research assistant, surpassing traditional SLRs in quality. Improvements are needed in high-quality data use, citation, and performance optimisation. GPT-4o, GPT-3.5, and Llama3-70b were the most suitable foundation models, but fine-tuning DELSTAR is essential to enhance sensitivity, especially critical in pharmaceutical contexts.
High Temperature Tolerant Mid-Wavelength Infrared Avalanche Photodiodes with Separated Fully-depleted Absorption, Multiplication Region
Authors: Zhu Liqi , Wang Zihao , Lin Jiamu , Huang Jian , He Linxuan , Wang Xi , Zhou Songmin , Gan Zhikai , Li Xun , Li Qingxin , He Li , Lin Changqing , Lin Chun , Chen Baile
Published: January 2025

Mid-wavelength infrared (MWIR) avalanche photodiodes (APD) are extensively employed in rapid, high-precision detection as well as thermal imaging in complex context due to their superior sensitivity, fast response, and high gain. However, conventional MWIR APD’s detection typically requires lowtemperature operation to relieve signal-to-noise limitations imposed by high dark currents in narrow bandgap materials. To address this challenge, we present the high-temperature-operating MWIR avalanche photodiode with a separated fullydepleted absorption, multiplication region (SFDAM) structure to suppress the diffusion dark current and minimize the excess noise. The proposed APD can cope with infrared light well up to 4.2 µm, and exhibits low dark current density at unit gain that nearly an order of magnitude lower than the theoretical diffusion current limitation at elevated temperatures of 160 K. At 80 K, the device achieves comparable gain-normalized dark current density (GNDCD) still <6×10-10A/cm 2 at gain < 20 and demonstrates low excess noise < 1.67. At a high temperature of 160 K, the GNDCD preserves consistently below 2×10−6 A/cm 2 for gain values less than 189, while the excess noise holds below 1.4 and the noise equivalent power is <7.2×10 −16 W/ Hz of 3.5 µm. The device is also validated for imaging weak targets up to 200 km away. These results enable the SFDAM APDs are promising and desirable for future high-temperature-operating MWIR detection and likely the photon-starved applications.
5G Performance and Enhancement with Dust Storm Conditions
Authors: Sh.Hammed Zainab , Ameen Siddeeq Y.
Published: January 2025

The increasing demand for ultra-fast data, high capacity, and low latency in 5G and beyond networks is driving the adoption of millimeter-Wave (mm-Wave) frequencies (3–300 GHz), which utilize spatial multiplexing and beamforming for improved performance. However, environmental factors like humidity, temperature, dust, and sandstorms, particularly in the Middle East, pose significant challenges. The parameters of the channel model and how it behaves statistically when exposed to dust and sandstorms have been analyzed using NYUSIM simulator and MATLAB. Wireless communication channels face challenges like time variability, frequency-selective fading, and interference from adjacent subcarriers, making traditional estimation methods less effective. This paper introduces a DNN model based on Bidirectional Long Short-Term Memory (BI-LSTM) networks, which excel at processing time-series data with long-range dependencies, outperforming standard RNNs. An end-to-end channel estimation and signal detection process using the Bi-LSTM-based detector is simulated and compared with traditional techniques such as LS, ZF, and MMSE. Results show that DNN provides higher estimation accuracy, especially with fewer pilots, achieving SER 30 to 35 times lower than ZF and MMSE. Additionally, the DNN model offers an SNR gain of approximately 10–15 dB or more compared to conventional methods. The proposed approach shows cases promising advancements for not only 5G but also for the evolving requirements of beyond-5G networks, offering a reliable solution for maintaining efficient Multiple-Input Multiple-Output Orthogonal Frequency Division Multiplexing MIMO-OFDM communication under diverse weather conditions.
In-silico Insights into Protein Targets: New Avenues for Treating E. faecalis in Endodontic Infections – A Systematic Review
Authors: V Swetha Geervani , Neelakantappa Kiran Kumar , Merwade Seema , M Abhishek , M Manimozhi
Published: January 2025

Enterococcus faecalis is a key pathogen in persistent endodontic infections, known for its resilience and resistance to conventional treatments. This systematic review (PROSPERO CRD42024610795) explores in silico methodologies targeting E. faecalis proteins to identify novel therapeutic approaches. A comprehensive literature search identified 11 relevant studies employing molecular docking tools such as AutoDock Vina, Glide XP, and SwissDock to investigate interactions between ligands and critical protein targets, including Sortase A, MurA, c-di-AMP synthetase, and quorum sensing regulators. Promising inhibitors, such as pinocembrin, 24-propylcholesterol, and embelin, exhibited potential to disrupt biofilm formation, quorum sensing, and bacterial metabolism. These findings highlight the potential of plant-derived compounds and novel antibacterial agents in addressing E. faecalis-associated infections. While most studies validated computational results with in vitro assays, variability in ligand preparation, protein optimization, and docking methodologies indicates the need for standardization. This review underscores the significant promise of computer-aided drug design (CADD) in developing effective treatments for E. faecalis infections. Integrating in silico and in vitro approaches can accelerate drug discovery, but further in vivo studies are essential to confirm therapeutic potential and facilitate clinical application.
WITHDRAWN: SUV39H1 alleviates MAFLD through inhibition of the FABPs/CEPT1 signaling pathway
Authors: sun yi , Wang changyuan , Wang Yang , Hao Ru , Liu Lu , Jin yue , Sun Huijun
Published: January 2025

Background
Epigenetics modification has emerged as a potential mechanism of action for MAFLD. The histone methylase SUV39H1 is a key epigenetic transcription factor that regulates adipogenesis and inflammation, but its potential role in MAFLD progression remains largely unknown.
Methods
In this study, the high fat diet (HFD) induced mice and palmitic acid (PA) induced primary mouse hepatocytes were adopted to investigate the effects and mechanisms of SUV39H1 on MAFLD. Point mutation and knockdown of SET domain of SUV39H1 in PA-stimulated AML12 cells and high-fat diet fed mice were adopted to confirm the function of SET domain in MAFLD. RNA–sequencing was used to screen the downstream genes affected by SUV39H1 overexpression in PA-induced AML12 cells. The functional relationship between SUV39H1 and its downstream FABPs was elucidated by CHIP, DNA Pull Down and dual-luciferase reporter assays.
Results
SUV39H1 could attenuate MAFLD by affecting lipid metabolism, suppressing inflammation and insulin resistance. And the SET domain of SUV39H1 was proved to be the functional domain mediating its effects on MAFLD in vivo and in vitro. Further studies revealed that overexpression of FABPs or CEPT1, the downstream genes of SUV39H1 could accelerate the disorder of lipid metabolism in MAFLD. Meanwhile, FABP could promote CEPT1 expression. Histone methylation modification of FABP promoters by SUV39H1 could inhibit the progression of MAFLD.In addition, FABPs derived from hepatocytes could aggravate the progression of MAFLD by promoting M1 polarization of the macrophages.
Conclusion
SUV39H1 alleviates MAFLD through inhibition of the FABPs/CEPT1 signaling pathway.
Genetically predicted blood metabolites mediate the association between circulating inflammation-related proteins and pancreatic cancer
Authors: Duan Yanheng , Zhang Fen , Zhang Xiaojing , Jin Liang , He Kun , Guan Yufan , Dong Xiaotian , Chen Yong , An Jiaze
Published: January 2025

Background: While extensive research highlighted the involvement of circulating inflammatory proteins and metabolism in pancreatic cancer (PC), causality remains unestablished. In the present study, we aimed to investigate the causal relationship of circulating inflammatory proteins and pancreatic cancer and identify the blood metabolites as potential mediators.
Methods: We employed bidirectional two-sample Mendelian randomization analysis to examine the potential causal association between circulating inflammatory proteins, circulating metabolites, and PC using data from genome-wide association studies (GWAS). And two-step MR to discover potential mediating blood metabolites in this process.
Results: MR analysis identified 4 types of circulating inflammation-related proteins causally associated with pancreatic cancer. Furthermore, there was no strong evidence that genetically predicted pancreatic cancer had an effect on these four types of circulating inflammatory proteins. Further two-step MR analysis found 11 types of blood metabolites were causally associated with pancreatic cancer and the associations between circulating Interleukin-15 receptor subunit alpha and pancreatic cancer were mediated by blood 5-methyluridine with proportions of 7.41%.
Conclusion: The present study provides evidence supporting the causal
relationships between various circulating inflammatory proteins, especially Interleukin-15 receptor subunit alpha, and pancreatic cancer, with a potential effect mediated by blood metabolites. Further research is needed on additional risk factors as potential mediators and establish a comprehensive inflammation-metabolism network in pancreatic cancer.
Deep Data-Driven Neural Network for Malaria Vaccination
Authors: Abaka-Quansah Esi , Moore Stephen E.
Published: January 2025

Malaria still remains a significant global health challenge that requires innovative strategies for its control and eventual eradication. In this article, we present a malaria vaccination model to assess and predict the effects of vaccination interventions. The model parameters are learned via feedforward Neural Network. We employed Residual Neural Network and Recurrent Neural Networks(GRU, LSTM, and BiLSTM) to predict and forecast daily and sequential malaria cases using generated data from the Ghana vaccination population.
Brain Tumor Classification in Sustainable Healthcare using Machine Learning
Authors: Patel Sanjay
Published: January 2025

In sustainable healthcare, early and accurate diagnosis is crucial in reducing the burden on healthcare systems and improving patient outcomes. This paper explores the application of machine learning techniques, specifically K-Nearest Neighbors (KNN) and Support Vector Classifier (SVC), for classifying brain tumors based on medical imaging data. By leveraging these machine learning methods, we aim to provide an efficient and interpretable solution that maintains high accuracy while optimizing computational resources. Machine learning models, due to their lower resource demands, are more suitable than deep learning approaches for sustainable healthcare environments, particularly in scenarios with limited infrastructure. The proposed models are validated through cross validation and hyperparameter tuning to achieve optimal performance. Our results demonstrate the potential of machine learning in brain tumor classification, offering a balance between accuracy and sustainability, and paving the way for more scalable and accessible diagnostic systems.
Tweak Promotes Neovascularization And Brain Damage Reduction In A Rat Model Of Intracerebral Hemorrhage
Authors: Romaus-Sanjurjo Daniel , López-Arias Esteban , Rodríguez Cristina , Hervella Pablo , Rodríguez-Arrizabalaga Mariña , Debasa-Mouce Manuel , Pías-Peleteiro Juan Manuel , Iglesias-Rey Ramón , Aguiar Pablo , Almeida Ángeles , Castillo José , Ouro Alberto , Sobrino Tomás
Published: January 2025

Non-traumatic intracerebral hemorrhage (ICH) is one of the most devastating and disabling forms of stroke; however, there are no effective pharmacological therapies following the insult. Angiogenesis appears as a key step to overcome the damage and promote functional recovery. In this context, endothelial progenitor cells (EPCs) mobilization promotes neovascularization which has been linked to beneficial outcomes following both ischemic and hemorrhagic stroke. The TNF-like weak inducer of apoptosis (TWEAK), binding to its receptor Fn14, has been suggested as an inducer of EPCs differentiation, viability and migration to the injury site in a model of myocardial infarction. Here, we have performed a proof-of-concept preclinical study in a rat model of ICH where we report that a 50 µg/kg dose of rat recombinant TWEAK (rTWEAK) promotes EPCs mobilization, as soon as 72 h post-injury, brain neovascularization, and, importantly, long-term hematoma reduction and functional recovery. In contrast, a higher dose of 150 µg/kg blocked those beneficial outcomes. Therefore, a low-dose of rTWEAK treatment promotes neovascularization and reduces brain damage in a rat model of ICH. Further clinical studies will be needed to demonstrate if rTWEAK could represent a new strategy to promote recovery following ICH.
Assessing the Impact of Eliminating Export Subsidies in Bangladesh
Authors: Rahman Mohammad Mohammad , Strutt Anna , Kim Chanwahn
Published: January 2025

The debate over export subsidy reform is crucial for many developing countries. This study analyses the impact of reducing or eliminating export subsidies for Bangladesh using a computable general equilibrium framework. Our simulations indicate that the partial removal of export subsidies has a positive effect on GDP. If export subsidies are eliminated only in the RMG sector real GDP increases by 0.04 per cent, which is similar to the full elimination of export subsidies by all sectors – perhaps not surprising given the significant contribution of this sector to GDP and that most subsidies apply to this sector. When export subsidies are reduced by 50 percent, with the funding transferred to seven targeted low-income household groups, real GDP is projected to increase by about 0.81 percent. Government transfers to households lead to an increase in real income for all seven targeted households, especially rural households, where incomes rise on average by 2.5 percent. This study indicates that there are significant inefficiencies and opportunity costs associated with export subsidies, and household income could be enhanced by redirecting the spending to more productive channels.
JEL Classification: F14, F17, F47.
JARIMAH BAGI PELAKU TINDAK PIDANA LINGKUNGAN HIDUP PERSPEKTIF HUKUM ISLAM
Authors: Haniah Rafiqatul
Published: January 2025

Lately in Indonesia there have been frequent natural disasters such as landslides. One of the causes of natural disasters is due to human actions that use nature carelessly, where humans in managing nature do not pay attention to ecosystems in a comprehensive and well-planned manner. Allah SWT has provided provisions that humans are encouraged to always take care of their environment as well as possible so that it remains sustainable. Human behavior that does not have a sense of responsibility and always causes damage to nature must certainly be punished accordingly (jarimah) for their actions. This paper aims to describe the exact form of jarimah imposed on the perpetrators of environmental destruction. Environmental destruction can be categorized as a criminal act. The purpose of imposing a finger on the perpetrators of environmental destruction is so that the perpetrators are wary of what they have done. The forms of jarimah are very diverse, therefore it is necessary to have an appropriate analysis in giving jarimah to perpetrators of environmental crimes.
DINAMIKA PARADIGMA FALAKIYAH NAHDLATUL ULAMA
Authors: Musonnif Ahmad
Published: January 2025

In determining the beginning of the Hijri month, Nahdlatul Ulama (NU) is known as a user of the rukyah al-hilal method with hisab as a support. However, after NU's 34th congress in Lampung in 2021, NU formulated the concept of qath'iy al-rukyah which is characterized by pure hisab. This shows the dynamics of the NU falakiyah paradigm. This study aims to describe chronologically and historically the dynamics of the NU falakiyah paradigm. The method used in this research is a literature search. The approach used in this research is Thomas S. Kuhn's paradigm theory to analyze how the NU falakiya paradigm develops. The results of this study indicate that in the history of NU, the NU falakiyah paradigm developed dynamically where at its inception there were elements of NU who used pure rukyah (normative paradigm) and some who used pure hisab (rational paradigm). This happens because there is no organizational stipulation to use a certain method in determining the beginning of the Hijri month. In the next period NU decided organizationally to use pure rukyah (normative paradigm), then use rukyah supported by hisab with imkan al-rukyah (normative-rational paradigm) but in certain cases NU used pure hisab with the criteria of qath'iy al-rukyah (paradigm purely rational). The dynamics of the falakiyah paradigm does not only occur organizationally in the NU falakiyah paradigm, but also occurs within the body of NU where there are a group of NU elements who use pure rukyah and also pure hisab which of course is not in accordance with the NU falakiyah paradigm organizationally.
Spectral properties of Levy Rosenzweig-Porter model via supersymmetric approach
Authors: Safonova Elizaveta , Feigel'man Mikhail V. , Kravtsov Vladimir
Published: January 2025

By using the Efetov’s super-symmetric formalism we computed analytically the mean spectral density \rho(E)ρ(E) for the Lévy and the Lévy -Rosenzweig-Porter random matrices which off-diagonal elements are strongly non-Gaussian with power-law tails. This makes the standard Hubbard-Stratonovich transformation inapplicable to such problems. We used, instead, the functional Hubbard-Stratonovich transformation which allowed to solve the problem analytically for large sizes of matrices. We show that \rho(E)ρ(E) depends crucially on the control parameter that drives the system through the transition between the ergodic and the fractal phases and it can be used as an order parameter.
Universal performance gap of neural quantum states applied to the Hofstadter-Bose-Hubbard model
Authors: Ledinauskas Eimantas , Anisimovas Egidijus
Published: January 2025

Neural Quantum States (NQS) have demonstrated significant potential in approximating ground states of many-body quantum systems, though their performance can be inconsistent across different models. This study investigates the performance of NQS in approximating the ground state of the Hofstadter-Bose-Hubbard (HBH) model, an interacting boson system on a two-dimensional square lattice with a perpendicular magnetic field. Our results indicate that increasing magnetic flux leads to a substantial increase in energy error, up to three orders of magnitude. Importantly, this decline in NQS performance is consistent across different optimization methods, neural network architectures, and physical model parameters, suggesting a significant challenge intrinsic to the model. Despite investigating potential causes such as wave function phase structure, quantum entanglement, fractional quantum Hall effect, and the variational loss landscape, the precise reasons for this degradation remain elusive. The HBH model thus proves to be an effective testing ground for exploring the capabilities and limitations of NQS. Our study highlights the need for advanced theoretical frameworks to better understand the expressive power of NQS which would allow a systematic development of methods that could potentially overcome these challenges.
AN EVALUATION OF DIFFICULTIES DURING RESEARCH BY THE POST GRADUATE STUDENTS IN A TERTIARY CARE HOSPITAL IN SOUTH KERALA
Authors: O. Geetha
Published: January 2025

The value and integrity of Research are dependent on the integrity of the researcher. They have the responsibilities to prevent Research misconduct and misuse of Research . All members of the research team are expected to maintain high standards and to uphold the fundamental values of research .Research is done by a person or a small group of persons . But it requires acceptance or approval of several others like Guide, supervisors , Institutional research Committee , Institutional ethics Committee etc . This might create a lot of hindrances or hardships for the researchers .

Objectives: 1.To find out the problems faced by the Researchers while conducting the postgraduate thesis/ dissertation 2.To formulate some support system for the PG students

Methods: All the Postgraduate students willing for the study were asked to fill up the questionnaire in google form .

Results: Selecting the topic of study, meeting the deadlines fixed by the authorities, getting materials for review of literature, getting adequate number of cases are the stages found difficult by the most of the participants. During the critical phase of postgraduate studies, they feel incompetent to choose a topic of study by their own. Difficulty is felt in selecting the relevant materials from the vast information available in internet and identifying standard journals. They have apprehension of rejection of simple studies by IRC and of questions putforth in IEC. They cannot dedicate enough time for research related work after their routine duties. They feel stressful when corrections suggested repeatedly by the committees and guides. Some of the suggestions made by them to reduce difficulties in research are (a) constant motivation by the guide, (b) multiple review meetings for follow up of the study, (c) Appraise the PG students to conduct research by discussing accepted thesis in methodology workshop instead of theory of Research. (d) Establishing a dedicated Research Helpline by Community Medicine Department.
Gestión flexible, subjetividad y experiencias paradojales de trabajo: el caso de Lean Management en el retail chileno
Authors: Garcés Ojeda Magdalena , Stecher Antonio
Published: January 2025

Este artículo presenta los resultados de un estudio que tiene como objetivo comprender elmodo en que la implementación de Lean management - una de las formas de gestiónflexible más difundidas a nivel mundial y latinoamericano – fue reconfigurando lasexperiencias subjetivas de trabajo, a partir de los cambios e innovaciones que introdujo anivel de las demandas técnicas y sociales del trabajo durante su implementación en laindustria del retail chileno. El estudio, de carácter analítico y descriptivo, contempló 26entrevistas a distintos consultores, gerentes, jefaturas y líderes sindicales. Desde el marcode las clínicas del trabajo, el artículo da cuenta del modo en que las particularidades de estaindustria inciden y son cruciales para comprender el proceso de reorganización del trabajoy los campos de experiencia subjetiva paradojales a los que dan lugar.