bluuebunny/crossref_metadata_2025 huggingface dataset:
School Medical Service: Strategies to Promote Psycho-Physiological Well-Being
Authors: Tafuri Francesco , Latino Francesca
Published: March 2024

Schools represent the ideal setting for educating children about the acquisition of active lifestyles seen not only from a health point of view but also from psycho-pedagogical and social perspectives. Based on evidence from scientific literature, there is a need to include physical activity in school routines, especially in primary schools, where the habits learned by children stay with them in their later years and adulthood. With the support of the school medicine service, schools become a favorable context for planning health education sessions aimed at students, with particular reference to prevention. Within teaching, it is necessary to consider the motor area as a fundamental tool for acquiring correct lifestyles, facilitating cognitive development, inclusiveness, and psycho-emotional and socio-relational factors. Schools can play a fundamental role, becoming the key to promoting physical activity at different times of the day, such as during class hours (with active breaks), during breaks, before and after lessons, and by integrating movement into teaching. This review is the result of an in-depth overview of the available literature on the relationship of schools with health and health promotion from a preventive perspective, with awareness of how the issue is being approached and the need for further future reflections that will go hand in hand with the coming changes.
Adenosine Increases the Immunosuppressive Capacity of Cervical Cancer Cells by Increasing PD-L1 Expression and TGF-β Production through Its Interaction with A2AR/A2BR
Authors: García-Rocha Rosario , Monroy-García Alberto , Vázquez-Cruz Ana Luisa , Marín-Aquino Luis Antonio , Weiss-Steider Benny , Hernández-Montes Jorge , Don-López Christian Azucena , Molina-Castillo Gabriela , Mora-García María de Lourdes
Published: March 2024

The present study provides evidence showing that adenosine (Ado) increases the expression of programmed death ligand 1 (PD-L1) in cervical cancer (CeCa) cells by interacting with A2AR/A2BR and that TGF-β1 acts in an autocrine manner to induce PD-L1 expression, enhancing the immunosuppressive effects of CeCa cells on activated T lymphocytes (ATLs) and CD8+ cytotoxic T lymphocytes (CTLs) specific for antigenic peptides derived from E6 and E7 proteins of HPV-16. Interestingly, the addition of the antagonists ZM241385 and MRS1754, which are specific for A2AR and A2BR, respectively, or SB-505124, which is a selective TGF-β1 receptor inhibitor, to CeCa cell cultures significantly inhibited PD-L1 expression. In addition, supernatants from CeCa cells that were treated with Ado (CeCa-Ado Sup) increased the expression of PD-1, TGF-β1, and IL-10 and decreased the expression of IFN-γ in ATLs. Interestingly, the addition of an anti-TGF-β neutralizing antibody strongly reversed the effect of CeCa-Ado Sup on PD-1 expression in ATLs. These results strongly suggest the presence of a feedback mechanism that involves the adenosinergic pathway, the production of TGF-β1, and the upregulation of PD-L1 expression in CeCa cells that suppresses the antitumor response of CTLs. The findings of this study suggest that this pathway may be clinically important and may be a therapeutic target.
Terpenes from Cecropia Species and Their Pharmacological Potential
Authors: Enchev Preslav , Zarev Yancho , Dakovska Anzhelica , Rivera-Mondragón Andrés , Kozuharova Ekaterina , Ionkova Iliana
Published: March 2024

Cecropia is a genus of neotropical trees mainly distributed in Mexico and Central and South America. Currently, 63 species have been described, some of which have been used for centuries in traditional medicine to treat conditions such as diabetes, high blood pressure, and wound healing, among others. In recent times, modern phytochemical studies have succeeded in isolating individual compounds with potential specific medicinal applications. This review aims to examine the literature data regarding isolated terpenes and their correlation with pharmacological activities, with the goal of unveiling the future potential of the genus.
Mannose-Decorated Solid-Lipid Nanoparticles for Alveolar Macrophage Targeted Delivery of Rifampicin
Authors: Bera Hriday , Zhao Caizhu , Tian Xidong , Cun Dongmei , Yang Mingshi
Published: March 2024

Alveolar macrophages play a vital role in a variety of lung diseases, including tuberculosis. Thus, alveolar macrophage targeted anti-tubercular drug delivery through nanocarriers could improve its therapeutic response against tuberculosis. The current study aimed at exploring the efficacy of glyceryl monostearate (GMS)-based solid-lipid nanoparticles (SLNs) and their mannose functionalized forms on the alveolar macrophage targeting ability of an anti-tubercular model drug, rifampicin (Rif). Rif-loaded SLNs were accomplished by the solvent diffusion method. These carriers with unimodal particle size distribution (~170 nm) were further surface-modified with mannose via Schiff-base reaction, leading to slight enhancement of particle diameter and a decline of drug loading capacity. The encapsulated Rif, which was molecularly dispersed within the matrices as indicated by their XRD patterns, was eluted in a sustained manner with an initial burst release effect. The uptake efficiency of mannose-modified SLNs was remarkably higher than that of corresponding native forms on murine macrophage Raw 264.7 cells and human lung adenocarcinoma A549 cells. Eventually, the mannose-modified SLNs showed a greater cytotoxicity on Raw 264.7 and A549 cells relative to their unmodified forms. Overall, our study demonstrated that mannose modification of SLNs had an influence on their uptake by alveolar macrophages, which could provide guidance for the future development of alveolar macrophage targeted nanoformulations.
The Laccase Family Gene CsLAC37 Participates in Resistance to Colletotrichum gloeosporioides Infection in Tea Plants
Authors: Li Dangqiang , Zhang Hongxiu , Zhou Qianqian , Tao Yongning , Wang Shuangshuang , Wang Pengke , Wang Aoni , Wei Chaoling , Liu Shengrui
Published: March 2024

Fungal attacks have become a major obstacle in tea plantations. Colletotrichum gloeosporioides is one of the most devastating fungal pathogens in tea plantations that can severely affect tea yield and quality. However, the molecular mechanism of resistance genes involved in anthracnose is still largely unknown in tea plants. Here, we found that the laccase gene CsLAC37 was involved in the response to fungal infection based on a transcriptome analysis. The full-length CDS of CsLAC37 was cloned, and its protein sequence had the closest relationship with the Arabidopsis AtLAC15 protein compared to other AtLACs. Tissue-specific expression analysis showed that CsLAC37 had higher expression levels in mature leaves and stems than in the other tissues. Subcellular localization showed that the CsLAC37 protein was predominantly localized in the cell membrane. The expression levels of CsLAC37 were upregulated at different time points under cold, salt, SA, and ABA treatments. qRT-PCR confirmed that CsLAC37 responded to both Pestalotiopsis-like species and C. gloeosporioides infections. Functional validation showed that the hydrogen peroxide (H2O2) content increased significantly, and POD activity decreased in leaves after antisense oligonucleotide (AsODN) treatment compared to the controls. The results demonstrated that CsLAC37 may play an important role in resistance to anthracnose, and the findings provide a theoretical foundation for molecular breeding of tea varieties with resistance to fungal diseases.
Species Identification and Genetic Diversity Analysis of Medicinal Plants Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz.
Authors: Sun Jing , Sun Qing , Li Xin , Li Wenjing , Li Yi , Zhou Yubi , Hu Yanping
Published: March 2024

The classification system for the genus Aconitum is highly complex. It is also the subject of ongoing debate. Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz. are perennial herbs of the genus Aconitum. Dried roots of these two plants are used in traditional Chinese medicine. In this study, morphological observations and ISSR molecular markers were employed to discriminate between A. flavum and A. pendulum, with the objective of gaining insights into the interspecies classification of Aconitum. The pubescence on the inflorescence of A. flavum was found to be appressed, while that on the inflorescence of A. pendulum was spread. UPGMA (unweighted pair-group method with arithmetic average) cluster analysis, PCoA (principal coordinates analysis), and Bayesian structural analysis divided the 199 individuals (99 individuals from DWM population and 100 individuals from QHL population) into two main branches, which is consistent with the observations of the morphology of pubescence on the inflorescence. These analyses indicated that A. flavum and A. pendulum are distinct species. No diagnostic bands were found between the two species. Two primer combinations (UBC808 and UBC853) were ultimately selected for species identification of A. flavum and A. pendulum. This study revealed high levels of genetic diversity in both A. flavum (He = 0.254, I = 0.395, PPB = 95.85%) and A. pendulum (He = 0.291, I = 0.445, PPB = 94.58%). We may say, therefore, that ISSR molecular markers are useful for distinguishing A. flavum and A. pendulum, and they are also suitable for revealing genetic diversity and population structure.
Effects of Invasive Alien Plant Species on Native Plant Diversity and Crop Yield
Authors: Najberek Kamil , Tokarska-Guzik Barbara , Chmura Damian , Solarz Wojciech
Published: March 2024

Invasive alien plant species may negatively affect the biological diversity of co-occurring native species, decrease the quality of invaded habitats, and even change the functioning of entire ecosystems [...]
Variation in Nutritional Components and Antioxidant Capacity of Different Cultivars and Organs of Basella alba
Authors: Zhang Yi , Cheng Wenjuan , Di Hongmei , Yang Shihan , Tian Yuxiao , Tong Yuantao , Huang Huanhuan , Escalona Victor Hugo , Tang Yi , Li Huanxiu , Zhang Fen , Sun Bo , Huang Zhi
Published: March 2024

Basella alba is a frequently consumed leafy vegetable. However, research on its nutritional components is limited. This study aimed to explore the variation in the nutritional components and antioxidant capacity of different cultivars and organs of Basella alba. Here, we primarily chose classical spectrophotometry and high-performance liquid chromatography (HPLC) to characterize the variation in nutritional components and antioxidant capacity among different organs (inflorescences, green fruits, black fruits, leaves, and stems) of eight typical cultivars of Basella alba. The determination indices (and methods) included the total soluble sugar (anthrone colorimetry), total soluble protein (the Bradford method), total chlorophyll (the ethanol-extracting method), total carotenoids (the ethanol-extracting method), total ascorbic acid (the HPLC method), total proanthocyanidins (the p-dimethylaminocinnamaldehyde method), total flavonoids (AlCl3 colorimetry), total phenolics (the Folin method), and antioxidant capacity (the FRAP and ABTS methods). The results indicated that M5 and M6 exhibited advantages in their nutrient contents and antioxidant capacities. Additionally, the inflorescences demonstrated the highest total ascorbic acid and total phenolic contents, while the green and black fruits exhibited relatively high levels of total proanthocyanidins and antioxidant capacity. In a comparison between the green and black fruits, the green fruits showed higher levels of total chlorophyll (0.77–1.85 mg g−1 DW), total proanthocyanidins (0.62–2.34 mg g−1 DW), total phenolics (15.28–27.35 mg g−1 DW), and ABTS (43.39–59.16%), while the black fruits exhibited higher levels of total soluble protein (65.45–89.48 mg g−1 DW) and total soluble sugar (56.40–207.62 mg g−1 DW) in most cultivars. Chlorophyll, carotenoids, and flavonoids were predominantly found in the leaves of most cultivars, whereas the total soluble sugar contents were highest in the stems of most cultivars. Overall, our findings underscore the significant influence of the cultivars on the nutritional composition of Basella alba. Moreover, we observed notable variations in the nutrient contents among the different organs of the eight cultivars, and proanthocyanidins may contribute significantly to the antioxidant activity of the fruits. On the whole, this study provides a theoretical basis for the genetic breeding of Basella alba and dietary nutrition and serves as a reference for the comprehensive utilization of this vegetable.
Prediction of Potential Suitable Areas and Priority Protection for Cupressus gigantea on the Tibetan Plateau
Authors: Zhang Huayong , Wei Yanan , Yue Junjie , Wang Zhongyu , Zou Hengchao , Ji Xiande , Zhang Shijia , Liu Zhao
Published: March 2024

Cupressus gigantea (C. gigantea) is an endemic endangered species on the Tibetan Plateau; its potential suitable areas and priority protection in the context of global climate change remain poorly predicted. This study utilized Biomod2 and Marxan to assess the potential suitable areas and priority protection for C. gigantea. Our study revealed that the suitable areas of C. gigantea were concentrated in the southeastern Tibetan Plateau, with the center in Lang County. Temperature was identified as a crucial environmental factor influencing the distribution of C. gigantea. Over the coming decades, the suitable range of C. gigantea expanded modestly, while its overall distribution remained relatively stable. Moreover, the center of the highly suitable areas tended to migrate towards Milin County in the northeast. Presently, significant areas for improvement are needed to establish protected areas for C. gigantea. The most feasible priority protected areas were located between the Lang and Milin counties in Tibet, which have more concentrated and undisturbed habitats. These results provide scientific guidance for the conservation and planning of C. gigantea, contributing to the stability and sustainability of ecosystems.
Green Extraction Approach for Isolation of Bioactive Compounds in Wild Thyme (Thymus serpyllum L.) Herbal Dust—Chemical Profile, Antioxidant and Antimicrobial Activity and Comparison with Conventional Techniques
Authors: Mrkonjić Živan , Kaplan Muammer , Milošević Sanja , Božović Danica , Sknepnek Aleksandra , Miletić Dunja , Lazarević Mrkonjić Ivana , Rakić Dušan , Zeković Zoran , Pavlić Branimir
Published: March 2024

The aim of this study was to provide a chemical profile and determine the antioxidant and antimicrobial activity of the essential oil (EO) and lipid extracts of Thymus serpyllum L. herbal dust obtained via conventional (hydrodistillation (HD) and Soxhlet extraction (SOX)) and novel extraction techniques (supercritical fluid extraction (SFE)). In addition, a comparative analysis of the chemical profiles of the obtained EO and extracts was carried out, as well as the determination of antioxidant, antibacterial and antifungal activity of the lipid extracts. According to the aforementioned antioxidant and antimicrobial activities and the monoterpene yield and selectivity, SFE provided significant advantages compared to the traditional techniques. In addition, SFE extracts could be considered to have great potential in terms of their utilization in the pharmaceutical and cosmetic industries, as well as appropriate replacements for synthetic additives in the food industry.
Study on Bearing Strength and Failure Modes of Single Bolted Joint Carbon/Epoxy Composite Materials
Authors: Park Sang Min , Jeon Jin Hwan , Choi Won Jong
Published: March 2024

The growth of the Urban Air Mobility (UAM) industry emphasizes the need for considerable study into assembly procedures and dependability to guarantee its effective integration into air transport networks. In this context, this study seeks to evaluate the mechanical characteristics of bolted joint Carbon Fiber Reinforced Plastic (CFRP), with a particular emphasis on bearing strength. By altering the w/D (specimen width to hole diameter) and e/D (distance between hole center and specimen end to hole diameter) ratios, the study investigates how edge and end distances affect material performance. The study discovered a shift from tension to bearing failure at w/D ratios of 4.0, with maximum bearing strength decreases of 90.50% and 69.96% compared to full bearing failure. Similarly, for e/D ratios of 1.5, 2.0, and 3.0, transitioning from shear to bearing failure at 2.0 resulted in maximum bearing strength losses of 94.90% and 75.96%, respectively. Maintaining a w/D ratio of at least 6.0 and an e/D ratio of at least 3.0 is critical for maintaining maximum performance and stability in CFRP structure design.
Effect of Pulping Waste Liquid on the Physicochemical Properties and the Prediction Model of Wheat Straw Residue Granular Fuels
Authors: Xue Lanxin , Yang Guihua , Lin Zhaoyun , Li Jinze , He Bo , Chen Jiachuan
Published: March 2024

Herein, wheat straw residue and pulping waste liquid were collected from pulping mill and mixed to prepare bio-based granular fuels by using compression molding technology, and to explore the comprehensive utilization of the industrial waste of pulping and papermaking. The effects of pulping waste liquid on granular fuel properties were analyzed systemically. Further study of the function of pulping waste liquid, cellulose and hemicellulose was used to replace wheat straw residue and avoid the interference factors. Therefore, the prediction models of granular fuels were established with influencing factors that included cellulose, hemicellulose and pulping waste liquid. The granular fuels had the best performance with 18.30% solid content of pulping waste liquid. The highest transverse compressive strength of granular fuel was 102.61 MPa, and the activation energy was 81.71 KJ·mol−1. A series of curve fitting prediction models were established to clarify the forming process of granular fuel, and it turned out that the pulping waste liquid could improve the adhesion between solid particles and increase their compression resistance.
Ethylene Scavenging Films Based on Ecofriendly Plastic Materials and Nano-TiO2: Preparation, Characterization, and In Vivo Evaluation
Authors: Maldonado Alba , Aguilar Tomas , Hauser Carolin , Wehnert Gerd , Söthje Dominik , Schlachter Herbert , Torres Alejandra , Bruna Julio , Valenzuela Ximena , Rodríguez-Mercado Francisco
Published: March 2024

It is known that ethylene plays an important role in the quality characteristics of fruits, especially in storage. To avoid the deterioration of fruits caused by ethylene, titanium dioxide (TiO2) has been used due to its photocatalytic capacity. The aim of this study was to develop films based on two types of biopolymers, Mater-Bi (MB) and poly-lactic acid (PLA), with nanoparticles of TiO2 and to determine their ethylene removal capacity and its application in bananas. First, the films were fabricated through an extrusion process with two different concentrations of TiO2 (5 and 10% w/w). Then, the films were characterized by their structural (FTIR), morphological (SEM), thermal (DSC and TGA), dynamic (DMA), barrier, and mechanical properties. The ethylene removal capacities of the samples were determined via gas chromatography and an in vivo study was also conducted with bananas for 10 days of storage. Regarding the characterization of the films, it was possible to determine that there was a higher interaction between PLA with nano-TiO2 than MB; moreover, TiO2 does not agglomerate and has a larger contact surface in PLA films. Because of this, a higher ethylene removal was also shown by PLA, especially with 5% TiO2. The in vivo study also showed that the 5% TiO2 films maintained their quality characteristics during the days in storage. For these reasons, it is possible to conclude that the films have the capacity to remove ethylene. Therefore, the development of TiO2 films is an excellent alternative for the preservation of fresh fruits.
Analysis of Multi-Fracture Extension Pattern of Horizontal Wells in Shale Reservoirs under Natural Fracture Perturbation
Authors: Wang Jianbo , Zhao Huan , Liu Huifang , Li Wei , Li Junru , Tang Pengfei , Zhang Minghui , Liu Yanling , Wang Siqi , Xu Xingsheng , He Tiansu
Published: March 2024

There are many natural fractures in shale reservoirs, changes in hydraulic fracture extension patterns. In the paper, a multi-fracture extension finite element model for horizontal wells in shale reservoirs under the disturbance of natural fractures is established by combining the actual geological parameters and construction parameters of a horizontal well multi-fracturing operation in X oilfield to analyze the effects of the difference in geostress, elastic modulus, angle of natural fractures, and the number of natural fracture groups on the hydraulic fracture extension. The results show that with the increase in ground stress difference and natural fracture angle, hydraulic fractures are more likely to penetrate the natural fractures; with the increase in elastic modulus, the fracture stress and tip stress increase, the volume of rupture unit, the fracture extension width and the pore pressure concentration area decrease, and it is easy to form a long and narrow fracture; with the increase in the number of fracture groups, the connectivity of reservoir fractures increases, the extension of fractures is stronger, and it is easier to form a complex fracture network.
Performance of a Solar-Driven Photocatalytic Membrane Reactor for Municipal Wastewater Treatment
Authors: Constantin Mirela Alina , Constantin Lucian Alexandru , Ionescu Ioana Alexandra , Nicolescu Cristina Mihaela , Bumbac Marius , Tiron Olga
Published: March 2024

The increasing demand for efficient wastewater treatment technologies, driven by global population growth and industrialisation, highlights the necessity for advanced, reliable solutions. This study investigated the efficacy of a slurry photocatalytic membrane reactor (PMR) for the advanced removal of organic pollutants, quantified via chemical oxygen demand (COD), under natural and simulated solar light irradiation. Employing two variants of iron-doped titania as photocatalysts and a polysulfone-based polymeric membrane for the separation process, the investigation showcased COD removal efficiencies ranging from 66–85% under simulated solar light to 52–81% under natural sunlight over a 7 h irradiation period. The overall PMR system demonstrated COD removal efficiencies of 84–95%. The results confirmed the enhanced photocatalytic activity afforded by iron doping and establish solar-powered slurry PMRs as an effective, low-energy, and environmentally friendly alternative for the advanced treatment of municipal wastewater, with the research providing valuable insights into sustainable water management practices.
An Experimental Investigation of the Dynamic Performances of a High Speed 4-DOF 5R Parallel Robot Using Inverse Dynamics Control
Authors: Righettini Paolo , Strada Roberto , Cortinovis Filippo , Tabaldi Federico , Santinelli Jasmine , Ginammi Andrea
Published: March 2024

High-speed pick-and-place industrial applications often use parallel kinematic robots due to their high stiffness and dynamic performance; furthermore, the latter not only depends on the mechanical characteristics of the robots but also on the control algorithm. The literature shows several theoretical contributions to such controllers, mainly tested at the simulation level or on simple proof-of-concept laboratory equipment that execute low-speed and simple trajectories. This paper presents an experimental investigation of the dynamic performance of an industrial high-speed 4-DOF 5R parallel robot designed for pick-and-place applications on moving objects. The inverse dynamics control in the task space is used as a control algorithm. The results show the contribution of all the components of the control algorithm to the motor torque, and the inverse dynamics controller performances are discussed also in comparison to those achievable with simpler PD or PID controllers in a joint space. Moreover, the paper shows the controller synthesis from a modern mechatronic point of view, and the effectiveness of the proposed solution for the tracking of complex high-speed trajectories in an industrial application.
Terminal Congestion Analysis of Container Ports Using Satellite Images and AIS
Authors: Yasuda Kodai , Shibasaki Ryuichi , Yasuda Riku , Murata Hiroki
Published: March 2024

This study proposes the use of satellite images and a vessel’s automatic identification system (AIS) data to evaluate the congestion level at container ports for operational efficiency analysis, which was never attempted in previous studies. The congestion level in container yards is classified by developing a convolutional neural network (CNN) model and an annotation tool to reduce the workload of creating training data. The annotation tool calculates the number of vertically stacked containers and the reliability of each container cell in a detection area by focusing on the shadows generated by the containers. Subsequently, a high-accuracy CNN model is developed for end-to-end processing to predict congestion levels. Finally, as an example of dynamic efficiency analysis of container terminals using satellite images, the relationship of the estimated average number of vertically stacked containers in the yard with the elapsed time between the image capture time and vessel arrival or departure time obtained from the automatic identification system data is analyzed. This study contributes to representing a prototype for dynamically estimating the number of vertically stacked containers and congestion level of container terminals using satellite images without statistical information, as well as its relationship with the timing of vessel arrival acquired from AIS data.
Monitoring of Cropland Abandonment and Land Reclamation in the Farming–Pastoral Zone of Northern China
Authors: Ye Junzhi , Hu Yunfeng , Feng Zhiming , Zhen Lin , Shi Yu , Tian Qi , Zhang Yunzhi
Published: March 2024

The farming–pastoral zone in northern China is one of the most ecologically sensitive areas globally, having experienced extensive cropland abandonment and land reclamation over decades, primarily influenced by policy adjustment and global warming. However, the spatiotemporal patterns and suitability of long-term cropland change remain poorly understood. Using the annual China land cover dataset (CLCD), we provide a cropland abandonment and land reclamation mapping approach based on actual land use processes (rather than land cover conditions) to investigate spatiotemporal features of abandonment and reclamation and evaluate the rationality. Our findings show that: (1) Returning farmland to forest and grassland has been a clear trend in the study area over the past 30 years. Specifically, cropland use has undergone three phases of change, i.e., cropland contraction and expansion alternately (before 2000), followed by substantial abandonment (after 2000), and low-intensity reclamation (after 2010). (2) In the last decade, the intensity of the abandonment of cropland with high and moderate suitability is low. The rate of abandonment decreased, while the intensity of land reclamation was relatively high. The rate of the reclamation increased, and the spatial distribution of cropland tended to be reasonable. Our study emphasizes the importance of monitoring actual cropland changes based on land use processes, and this method can be effectively extended to regional or global long-term cropland monitoring.
Active Deformation Areas of Potential Landslide Mapping with a Generalized Convolutional Neural Network
Authors: Wu Qiong , Ge Daqing , Yu Junchuan , Zhang Ling , Ma Yanni , Chen Yangyang , Wan Xiangxing , Wang Yu , Zhang Li
Published: March 2024

Early discovery and monitoring of the active deformation areas of potential landslides are important for geohazard risk prevention. The objective of the study is to propose a one-step strategy for automatically mapping the active deformation areas of potential landslides from a Sentinel-1 SAR dataset. First, we built a generalized convolutional neural network (CNN) based on activity and topographic characteristics. Second, we conducted a comparative analysis of the performance of various multi-channel combiners for detecting the active deformation areas of the potential landslides. Third, we verified the transferability of the pretrained CNN model for an unknown region. We found that by incorporating topographic characteristics into a generalized convolutional neural network, we were able to enhance the accuracy of identifying the active deformation areas of potential landslides, rapidly mapping these areas. The methodology is robust and efficient, and it has the capability to automatically detect the active deformation areas of potential landslides, even in unknown or unfamiliar regions. This product can facilitate automated pipelines, updating and mapping active deformation areas for final users who are not InSAR experts. This implementation can be used for providing support to risk management activities.
A New Technique for Urban and Rural Settlement Boundary Extraction Based on Spectral–Topographic–Radar Polarization Features and Its Application in Xining, China
Authors: Li Xiaopeng , Zhou Guangsheng , Zhou Li , Lv Xiaomin , Li Xiaoyang , He Xiaohui , Tian Zhihui
Published: March 2024

Highly accurate data on urban and rural settlement (URS) are essential for urban planning and decision-making in response to climate and environmental changes. This study developed an optimal random forest classification model for URSs based on spectral–topographic–radar polarization features using Landsat 8, NASA DEM, and Sentinel-1 SAR as the remote-sensing data sources. An optimal urban and rural settlement boundary (URSB) extraction technique based on morphological and pixel-level statistical methods was established to link discontinuous URSs and improve the accuracy of URSB extraction. An optimal random forest classification model for URSs was developed, as well as a technique to optimize URSB, using the Google Earth Engine (GEE) platform. The URSB of Xining, China, in 2020 was then extracted at a spatial resolution of 30 m, achieving an overall accuracy and Kappa coefficient of 96.21% and 0.92, respectively. Compared to using a single spectral feature, these corresponding metrics improved by 16.21% and 0.35, respectively. This research also demonstrated that the newly constructed Blue Roof Index (BRI), with enhanced blue roof features, is highly indicative of URSs and that the URSB was best extracted when the window size of the structural elements was 13 × 13. These results can be used to provide technical support for obtaining highly accurate information on URSs.
Nearshore Ship Detection in PolSAR Images by Integrating Superpixel-Level GP-PNF and Refined Polarimetric Decomposition
Authors: Wu Shujie , Wang Wei , Deng Jie , Quan Sinong , Ruan Feng , Guo Pengcheng , Fan Hongqi
Published: March 2024

Nearshore ship detection has significant applications in both the military and civilian domains. Compared to synthetic aperture radar (SAR), polarimetric synthetic aperture radar (PolSAR) provides richer information for analyzing the scattering mechanisms of ships and enables better detection of ship targets. However, ships in nearshore areas tend to be highly concentrated, and ship detection is often affected by adjacent strong scattering, resulting in false alarms or missed detections. While the GP-PNF detector performs well in PolSAR ship detection, it cannot obtain satisfactory results in these scenarios, and it also struggles in the presence of azimuthal ambiguity or strong clutter interference. To address these challenges, we propose a nearshore ship detection method named ECD-PNF by integrating superpixel-level GP-PNF and refined polarimetric decomposition. Firstly, polarimetric superpixel segmentation and sea–land segmentation are performed to reduce the influence of land on ship detection. To estimate the sea clutter more accurately, an automatic censoring (AC) mechanism combined with superpixels is used to select the sea clutter superpixels. By utilizing refined eight-component polarimetric decomposition to improve the scattering vector, the physical interpretability of the detector is enhanced. Additionally, the expression of polarimetric coherence is improved to enhance the target clutter ratio (TCR). Finally, this paper combines the third eigenvalue of eigenvalue–eigenvector decomposition to reduce the impact of azimuthal ambiguity. Three spaceborne PolSAR datasets from Radarsat-2 and GF-3 are adopted in the experiments for comparison. The proposed ECD-PNF method achieves the highest figure of merit (FoM) value of 0.980, 1.000, and 1.000 for three datasets, validating the effectiveness of the proposed method.
Exploring Public Space Satisfaction in Old Residential Areas Based on Impact-Asymmetry Analysis
Authors: Chen Nuo , Fang Dewei
Published: March 2024

The renovation of public space environments in old residential areas has become the focal point in Chinese urban renewal and promotion of urban sustainable development; thus, an urgent need for research expansion is attached to identifying the environmental factors of public space and their impacts from the perspective of residents’ needs and satisfaction. Taking Hefei, China, as an example, and based on a satisfaction survey of the public space environment in old residential areas, this study discerned critical factors affecting public space satisfaction through gradient-boosting decision trees and impact-asymmetry analysis methods, after which the asymmetric relationship between public space environment factors and resident satisfaction was tested and the priority and goal of public space environment renovation were obtained. The results indicate that the following: (1) Compared with physical environment factors, current social environment factors, including uncivilized behavior, space occupation, and hygiene and cleanliness, exert a greater impact on the overall satisfaction. (2) The hypothesis that there exists a general nonlinear relationship between environmental factors and satisfaction is verified, with all social environment factors proving to be nonlinear and physical environment factors being highly related to social environment creation, such as nursing space for the old and young, reading and learning space, and display and communication space. (3) A priority hierarchy for the improvement of environmental factors should be established, which basically conforms to Maslow’s hierarchy of needs theory. The role of social environment renovation should be highlighted, along with the necessity to bolster community governance and public space management. At the level of the physical environment, more space should be available for the activities of residents, display and communication, and reading and learning. This study can provide a corresponding theoretical basis and planning inspiration for meeting the multiple needs of residents for public space, improving life happiness, and promoting the sustainable development of cities in the renovation of public space environments in old residential areas.
Manor and Park Estates—Resilience to Transformation and the New Management of Space Due to Political Changes: The Case of Western Pomerania (Poland)
Authors: Rzeszotarska-Pałka Magdalena
Published: March 2024

The historical landscape reveals diverse social, economic, political transformations that create an identity of place, one which should be protected for future generations. Manor and park estates have been and continue to be valuable parts of cultural heritage and a distinguishing feature of the rural landscape in Europe. Since 1945, however, they have often failed to resist negative changes, especially in Eastern Europe where, after the Second World War, countries adopted the socialist system, which abolished private property and placed many historical buildings under the management of state institutions for several decades. In this context, it is important to study their current state of preservation. This study analyzes the condition of manor and park estates in Western Pomerania, Poland, that became the property of State Agricultural Farms (PGRs) after the Second World War. For this purpose, it was necessary to determine the extent of their transformation brought about by the State Agricultural Farms from 1949–1991, as well as changes that have occurred in the past 30 years. A qualitative method was used to assess the preservation of historical manor and park estates and to classify them according to their degree of preservation. The results showed that 42% of the estates surveyed have had their original form and spatial composition transformed, and their preservation status is assessed as moderate. Nearly 30% are in poor condition, which is hindering their full revitalization and the restoration of cultural and natural values. Only 28% of manor and park estates are maintained in a very good state. It is a matter of concern that the decline of these estates has been continuous and that restoration efforts have been limited. However, by assessing the current state of preservation, we can identify the measures necessary to stop further deterioration and preserve the cultural heritage of the region. It is clear that the preservation of these estates is essential to maintaining the identity of Western Pomerania. Manor and park estates are historical monuments associated with the people who once lived there. They must be protected to promote sustainable development and preserve a common European cultural heritage for future generations.
The Utility of Spectroscopic MRI in Stereotactic Biopsy and Radiotherapy Guidance in Newly Diagnosed Glioblastoma
Authors: Rejimon Abinand C. , Ramesh Karthik K. , Trivedi Anuradha G. , Huang Vicki , Schreibmann Eduard , Weinberg Brent D. , Kleinberg Lawrence R. , Shu Hui-Kuo G. , Shim Hyunsuk , Olson Jeffrey J.
Published: March 2024

Current diagnostic and therapeutic approaches for gliomas have limitations hindering survival outcomes. We propose spectroscopic magnetic resonance imaging as an adjunct to standard MRI to bridge these gaps. Spectroscopic MRI is a volumetric MRI technique capable of identifying tumor infiltration based on its elevated choline (Cho) and decreased N-acetylaspartate (NAA). We present the clinical translatability of spectroscopic imaging with a Cho/NAA ≥ 5x threshold for delineating a biopsy target in a patient diagnosed with non-enhancing glioma. Then, we describe the relationship between the undertreated tumor detected with metabolite imaging and overall survival (OS) from a pilot study of newly diagnosed GBM patients treated with belinostat and chemoradiation. Each cohort (control and belinostat) were split into subgroups using the median difference between pre-radiotherapy Cho/NAA ≥ 2x and the treated T1-weighted contrast-enhanced (T1w-CE) volume. We used the Kaplan–Meier estimator to calculate median OS for each subgroup. The median OS was 14.4 months when the difference between Cho/NAA ≥ 2x and T1w-CE volumes was higher than the median compared with 34.3 months when this difference was lower than the median. The T1w-CE volumes were similar in both subgroups. We find that patients who had lower volumes of undertreated tumors detected via spectroscopy had better survival outcomes.
Single-Nucleotide Polymorphisms Associated with Mercury Levels and Neurological Symptoms: An Overview
Authors: Perini Jamila Alessandra , Cardoso Jessica Vilarinho , Knesse Alana de Oliveira , Pessoa-Silva Felipe Oliveira , Vasconcellos Ana Claudia Santiago de , Machado Daniel Escorsim , Basta Paulo Cesar
Published: March 2024

Mercury (Hg) pollution is a global public health concern because of its adverse effects on the environment and health. Single-nucleotide polymorphisms (SNPs) have been associated with Hg levels and outcomes. The aim of this review was to describe the research and discuss the evidence on the genetic susceptibility of Hg-exposed individuals to the development of neurocognitive disorders. A systematic review was performed to identify the genes/SNPs associated with Hg toxicokinetics and that, therefore, affect neurological function in exposed populations. Observational and experimental studies were identified by screening three databases. Thirteen articles were included (quality score 82–100%) and 8124 individuals were evaluated. Hg exposure was mainly fish consumption (77%) and, in 31% of the studies, the Hg levels exceeded the reference limits. Genetic susceptibility to higher Hg levels and neurotoxicity risk in Hg poisoning were associated with eight (ALAD rs1800435, CYP3A4 rs2740574, CYP3A5 rs776746, CYP3A7 rs2257401, GSTP1 rs1695, MT1A rs8052394, MT1M rs2270836, and MT4 rs11643815) and three (MT1A rs8052394, MT1M rs2270837, and MT2A rs10636) SNPs, respectively, and rs8052394 was associated with both outcomes. The MT1A rs8052394 SNP may be used as a susceptibility biomarker to identify individuals at greater risk for higher Hg levels and the development of neurocognitive disorders in metal-exposed populations.
Host-like RNA Elements Regulate Virus Translation
Authors: Khan Debjit , Fox Paul L.
Published: March 2024

Viruses are obligate, intracellular parasites that co-opt host cell machineries for propagation. Critical among these machineries are those that translate RNA into protein and their mechanisms of control. Most regulatory mechanisms effectuate their activity by targeting sequence or structural features at the RNA termini, i.e., at the 5′ or 3′ ends, including the untranslated regions (UTRs). Translation of most eukaryotic mRNAs is initiated by 5′ cap-dependent scanning. In contrast, many viruses initiate translation at internal RNA regions at internal ribosome entry sites (IRESs). Eukaryotic mRNAs often contain upstream open reading frames (uORFs) that permit condition-dependent control of downstream major ORFs. To offset genome compression and increase coding capacity, some viruses take advantage of out-of-frame overlapping uORFs (oORFs). Lacking the essential machinery of protein synthesis, for example, ribosomes and other translation factors, all viruses utilize the host apparatus to generate virus protein. In addition, some viruses exhibit RNA elements that bind host regulatory factors that are not essential components of the translation machinery. SARS-CoV-2 is a paradigm example of a virus taking advantage of multiple features of eukaryotic host translation control: the virus mimics the established human GAIT regulatory element and co-opts four host aminoacyl tRNA synthetases to form a stimulatory binding complex. Utilizing discontinuous transcription, the elements are present and identical in all SARS-CoV-2 subgenomic RNAs (and the genomic RNA). Thus, the virus exhibits a post-transcriptional regulon that improves upon analogous eukaryotic regulons, in which a family of functionally related mRNA targets contain elements that are structurally similar but lacking sequence identity. This “thrifty” virus strategy can be exploited against the virus since targeting the element can suppress the expression of all subgenomic RNAs as well as the genomic RNA. Other 3′ end viral elements include 3′-cap-independent translation elements (3′-CITEs) and 3′-tRNA-like structures. Elucidation of virus translation control elements, their binding proteins, and their mechanisms can lead to novel therapeutic approaches to reduce virus replication and pathogenicity.
Investigation of the Correlation between Enterovirus Infection and the Climate Factor Complex Including the Ping-Year Factor and El Niño-Southern Oscillation in Taiwan
Authors: Yu Hsueh-Wen , Kuan Chia-Hsuan , Tseng Liang-Wei , Chen Hsing-Yu , Tsai Meg-Yen , Chen Yu-Sheng
Published: March 2024

Enterovirus infection and enterovirus infection with severe complications (EVSC) are critical issues in several aspects. However, there is no suitable predictive tool for these infections. A climate factor complex (CFC) containing several climate factors could provide more effective predictions. The ping-year factor (PYF) and El Niño-Southern Oscillation (ENSO) are possible CFCs. This study aimed to determine the relationship between these two CFCs and the incidence of enterovirus infection. Children aged 15 years and younger with enterovirus infection and/or EVSC were enrolled between 2007 and 2022. Each year was categorized into a ping-year or non-ping-year according to the PYF. Poisson regression was used to evaluate the associations between the PYF, ENSO, and the incidence of enterovirus infection. Compared to the ping-year group, the incidence rate of enterovirus infection, the incidence rate of EVSC, and the ratio of EVSC in the non-ping-year group were 1.24, 3.38, and 2.73 times higher, respectively (p < 0.001). For every one-unit increase in La Niña, the incidence rate of enterovirus infection decreased to 0.96 times (p < 0.001). Our study indicated that CFCs could be potential predictors for enterovirus infection, and the PYF was more suitable than ENSO. Further research is needed to improve the predictive model.
Seroprevalence for Measles, Varicella, Mumps and Rubella in the Trainee Obstetric Population: A Survey in Southern Italy
Authors: Perfetto Brunella , Paduano Giovanna , Grimaldi Elena , Sansone Vincenza , Donnarumma Giovanna , Di Giuseppe Gabriella
Published: March 2024

Background: Despite the efforts of the World Health Organization, some childhood viral diseases, for which there is already an effective vaccine, have not yet been eradicated. Among these, we find varicella, mumps, measles, and rubella, which although in most cases have a benign course, can in some cases be responsible for infectious outbreaks, especially in nosocomial settings. The aim of this study was to verify the immunological situation of a cohort of trainee obstetricians in Campania regarding varicella, mumps, measles, and rubella to be used as an example for the evaluation of possible preventive strategies to avoid infectious outbreaks. Methods: All the samples collected and sent to the laboratory were eligible for analysis and have been included in the study. Specific IgG for varicella, measles, mumps, and rubella were assayed on serum samples taken from 517 trainee obstetricians using the enzyme-linked immunosorbent assay (ELISA) technique. The seropositivity results were statistically analyzed by correlating them to age group and gender. Results: The results obtained show that a percentage of trainee obstetricians tested do not have an effective immunological coverage against at least one of the vaccine-preventable diseases considered, especially for mumps. Conclusions: Therefore, it is proposed to extend surveillance to other professionals in contact with frail patients and increase awareness of vaccination campaigns.