bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship between Remotely Sensed Ambient PM10 and PM2.5 and Urban Forest in Seoul, South Korea
Authors: Park Jincheol , Lee Peter Sang-Hoon
Published: October 2020

Currently particulate matter (PM) is one of the major threats to public health and safety in urban areas such as Seoul, South Korea. The limited amount of air-quality monitoring systems may not provide sufficient data or coverage, in particular on the spots of urban forest. Considering urban forest as a possible contributor to mitigate PM in an urban area, this study investigated the relationship between the size and topography of urban forests near the air-quality monitoring stations and PM measurements from those stations. The average of PM measurements during the study period of August 2017 to July 2019 was computed into three different domains by using three concentric buffers from 25 monitoring stations distributed across Seoul. To estimate PM concentrations, multiple linear regression models were developed by using satellite-borne multi-spectral band data retrieved from Moderate Resolution Imaging Spectroradiometer onboard Terra (MODIS) and Landsat 8 in conjunction with meteorological data sets. Overall, PM10 and PM2.5 measurements significantly varied with season and tended to be lower with large urban forests than small ones by 5.3% for PM10 and 4.8% for PM2.5. Overall, PM10 and PM2.5 measurements were lower at the domains encompassing high urban forests in elevation than those of relatively flattened forests by 9.1% for PM10 and 3.9% for PM2.5. According to the findings from this study, the topographical difference among urban forests could exert a more significant influence on PM mitigation. The result from correlation analysis between the PM estimates from Landsat 8-based models and ground-based PM measurements was considered reliable based on Pearson’s coefficients of 0.21 to 0.74 for PM10 and −0.33 to 0.74 for PM2.5. It was considered that using a satellite imagery-derived PM model could be effective to manage urban forest over a large area which in general implies the limitation of data collection.
Participatory Assessment of Ecosystem Services from Community-Managed Planted Forests in Bhutan
Authors: Rai Prem Bahadur , Sears Robin R. , Dukpa Dorji , Phuntsho Sonam , Artati Yustina , Baral Himlal
Published: October 2020

Planted forests provide a range of ecosystem services, from the local to global scales. In this study, we evaluated the perceptions by local people of the ecosystem services from planted forest stands in Bhutan. We employed household questionnaires, interviews and focus group discussions in eight villages in two regions of Bhutan: Trashigang dzongkhag, in eastern Bhutan, and Punakha dzongkhag in western Bhutan. At all sites, the planted forests were created several decades ago to restore forest cover in deforested and degraded areas. The restored stands were perceived by residents to provide multiple goods and ecosystem services important to local security, health, and livelihoods, including wild edibles, freshwater, and soil protection, and aesthetic and cultural services. This study indicates that local community participation in forest resource management can ease user conflict and manage the planted forests sustainably. We see community-based forest restoration on degraded lands as a viable approach, among others, to achieve national commitments to the Bonn Challenge and other multilateral environmental agreements related to forests.
Identifying Tree Traits for Cooling Urban Heat Islands—A Cross-City Empirical Analysis
Authors: Helletsgruber Carola , Gillner Sten , Gulyás Ágnes , Junker Robert R. , Tanács Eszter , Hof Angela
Published: October 2020

Research Highlights: This paper presents a cross-city empirical study on micro-climatic thermal benefits of urban trees, using machine-learning analysis to identify the importance of several in situ measured tree physiognomy traits for cooling. Background and Objectives: Green infrastructure and trees in particular play a key role in mitigating the urban heat island (UHI) effect. A more detailed understanding of the cooling potential of urban trees and specific tree traits is necessary to support tree management decisions for cooling our progressively hot cities. The goal of this study was to identify the influence and importance of various tree traits and site conditions. Materials and Methods: Surface temperature, air temperature at 1.1 m and at tree crown height, as well as wet bulb globe-temperature of shaded and fully sun-exposed reference areas, were used to study the cooling effect of seven different urban tree species. For all 100 individuals, tree height, crown base, trunk circumference, crown volume, crown area, leaf area index (LAI) and leaf area density (LAD) were measured. Measurements were conducted in the cities of Dresden, Salzburg, Szeged, and Vienna as representatives for middle European cities in different climate zones. Results: Beside site conditions, tree species, height, height of crown base, as well as trunk circumference, have a great influence on the cooling effect for city dwellers. The trunk circumference is a very valuable indicator for estimating climate regulating ecosystem services and therefore a highly robust estimator for policy makers and tree management practitioners when planning and managing urban green areas for improving the availability and provision of ecosystem services.
Spent Hen Protein Hydrolysate with Good Gastrointestinal Stability and Permeability in Caco-2 Cells Shows Antihypertensive Activity in SHR
Authors: Fan Hongbing , Yu Wenlin , Liao Wang , Wu Jianping
Published: October 2020

Spent hens are a major byproduct of the egg industry but are rich in muscle proteins that can be enzymatically transformed into bioactive peptides. The present study aimed to develop a spent hen muscle protein hydrolysate (SPH) with antihypertensive activity. Spent hen muscle proteins were hydrolyzed by nine enzymes, either individually or in combination; 18 SPHs were assessed initially for their in vitro angiotensin-converting enzyme (ACE) inhibitory activity, and three SPHs, prepared by Protex 26L (SPH-26L), pepsin (SPH-P), and thermoase (SPH-T), showed promising activity and peptide yield. These three hydrolysates were further assessed for their angiotensin-converting enzyme 2 (ACE2) upregulating, antioxidant, and anti-inflammatory activities; only SPH-T upregulated ACE2 expression, while all three SPHs showed antioxidant and anti-inflammatory activities. During simulated gastrointestinal digestion, ACE2 upregulating, ACE inhibitory and antioxidant activities of SPH-T were not affected, but those of SPH-26L and SPH-P were reduced. ACE inhibitory activity of gastrointestinal-digested SPH-T was not affected after the permeability study in Caco-2 cells, while ACE2 upregulating, antioxidant and anti-inflammatory activities were improved; nine novel peptides with five–eight amino acid residues were identified from the Caco-2 permeate. Among these three hydrolysates, only SPH-T reduced blood pressure significantly when given orally at a daily dose of 1000 mg/kg body weight to spontaneously hypertensive rats. SPH-T can be developed into a promising functional food ingredient against hypertension, contributing to a more sustainable utilization for spent hens while generating extra revenue for the egg industry.
Targeted Metabolomic and Transcript Level Analysis Reveals Quality Characteristic of Chinese Wild Grapes (Vitis davidii Foex)
Authors: Ju Yan-lun , Yue Xiao-feng , Cao Xue-ying , Fang Yu-lin
Published: October 2020

Native to China, spine grapes (Vitis davidii Foex) are an important wild grape species. Here, the quality characteristics of one white and three red spine grape clones were evaluated via targeted metabolomic and transcription level analysis. Xiangzhenzhu (XZZ) had the highest soluble sugar and organic acid content. Malvidin-3-acetyl-glucoside and cyanidin-3-glucoside were the characteristic anthocyanins in spine grapes, and significant differences in anthocyanin composition between different clones were detected. Anthocyanins were not detected in Baiyu (BY) grapes. The transcript levels of VdGST, VdF3′H, VdOMT, VdLDOX, and VdUFGT were significantly related to the anthocyanin biosynthesis and proportions. A total of 27 kinds of glycosidically bound volatiles (including alcohols, monoterpenes, esters, aldehydes, ketones, and phenolic acid) were identified in spine grapes, with Gaoshan #4 (G4) and BY grapes having the highest concentrations. The VdGT expression levels were closely related to glycosidically bound volatile concentrations. These results increase our understanding of the quality of wild spine grapes and further promote the development and use of wild grape resources.
All-Time Best Norwegian Track and Field Athletes: To What Extent Did They Achieve Outstanding Results at The Ages of 15 and 18 Years?
Authors: Tjelta Leif Inge , Tjensvoll Ola
Published: September 2020

The aim of the study was to determine how many Norwegian athletes who, during all the times they had achieved the European Athletics Championship 2020—Entry Standards (EAC20ES), were also ranked among the 20 all-time-best athletes at the ages of 15 and 18 years. The number of athletes who achieved the EAC20ES during their career, and the percentage of those who were among top 20 in the age groups 15 and 18 years, were determined from the Norwegian all-time-best results lists. A total of 202 athletes achieved the EAC20ES in the studied time period. Of these, 14.4% and 42.1% were ranked among the top 20 all-time best in one or more events at the ages of 15 and 18 years, respectively. However, among those who had won an international gold medal, these percentages were much higher. Eight out of 12 champions (66.7%) were ranked among the top 20 all-time best in one or more event at 15 years of age, and 11 of 12 champions (91.6%) were ranked among the top 20 all-time best at 18 years of age. Athletes that went on to win international championships typically performed better as adolescents compared to other athletes who also reach an international level as seniors. However, due to the low number of international champions, the date should be interpreted with caution.
Body Composition and a School Day Hydration State among Polish Children—A Cross-Sectional Study
Authors: Kozioł-Kozakowska Agnieszka , Piórecka Beata , Suder Agnieszka , Jagielski Paweł
Published: October 2020

Background: Little is known on the relationship between obesity and hydration level in children. To explore the possible association between children’s hydration status and body composition, we conducted this cross-sectional study. Methods: The survey was carried out in 2018 in Preliminary and High Schools from the Malopolska Province, Poland. The study group consisted of 264 children aged 7–15 years. The level of hydration was assessed based on urine osmolality during a school day. The examined had anthropometric tests and body composition assessment (FM, BF%, FFM, TBW, TBW%). Odds ratio (OR) and 95% confidence interval (CI) were calculated using a logistic regression analysis. Results: In the study group, 9.5% of the examined were overweight, 7.2% obese, and it referred more to the country than towns (p < 0.05). Improper hydration was found in 53% of children, and 16.3% of them were severely dehydrated during a school day (urine osmolality > 1000 mOsm/kgH2O). The level of dehydration was higher in children with excessive body fat (BF%) than in children with normal BF% [903.00 vs. 775 mOsm/kgH2O]. Older age (>10 y) showed inverse association with dehydration [OR 0.52 (95% CI; 0.28–0.99)] and excessed BF% showed 2.3-fold increase in odds of dehydration during a school day [OR 2.39 (95% CI; 1.15–4.94)]. Improper hydration was a risk factor of difficulties with concentration declared by students during a school day OR 2.85 (95% CI; 1.16–6.99). Conclusions: Attention should be paid to appropriate hydration especially in children with excessive body fat content who feature a higher risk of dehydration and fluid demand.
The Effect of Self-Reported Lactose Intolerance and Dairy Consumption on Bone Mineral Density among American Hip Arthroplasty Patients: A Cross-Sectional Study
Authors: Hamilton Nikola K. , Ojo Omorogieva , Adegboye Amanda Rodrigues Amorim
Published: October 2020

The relationship between osteoporosis and lactose intolerance is unclear. This study aims to evaluate the association between self-reported lactose intolerance and symptom severity caused by lactose malabsorption and bone mineral density (BMD). A total of 496 American hip arthroplasty patients took part in this study. Information on BMD and socio-demographic factors were retrospectively extracted from medical records. BMD of the lumbar spine (LS), femoral neck of the operative hip (FNOH), and femoral neck of the non-operative hip (FNH) were measured via dual-energy x-ray absorptiometry scans (DXA). Patients also completed a survey regarding dietary and lifestyle habits from the time of surgery. We found that 9.3% of participants reported lactose intolerance and 33.3%% suffered from either osteopenia or osteoporosis in at least one (location). The population that did not self-identify as lactose intolerant consumed significantly more dairy (p < 0.0001) and animal protein (p = 0.004) than those with intolerance. There was no significant difference in BMD between self-identified lactose intolerant individuals and non-lactose intolerant individuals. In a stepwise multiple regression analysis, body mass index (BMI) and age were the only common predictors of BMD for all locations (p < 0.05). However, yogurt intake was a significant predictor of BMD of FNOH in the multivariate analysis. This study suggests that lactose intolerance is not associated with bone mineral density. We also found that being vegan or vegetarian may increase the risk of low BMD.
Prognostic Factors for Staying at Work for Partially Sick-Listed Workers with Subjective Health Complaints: A Prospective Cohort Study.
Authors: Weerdesteijn Kristel , Schaafsma Frederieke , Bonefaas-Groenewoud Karin , Heymans Martijn , Van der Beek Allard , Anema Johannes
Published: October 2020

Examination of prognostic factors for staying at work for long-term sick-listed workers with subjective health complaints (SHC) who partially work in a paid job, and to evaluate whether these factors are comparable with those of workers with other disorders. We used data of 86 partially sick-listed workers with SHC (57 females, 29 males, mean age 47.1 years) and 433 with other disorders (227 females, 206 males, mean age 50.9 years), from an existing prospective cohort study consisting of 2593 workers aged 18–65 years and registered as sick-listed with different health complaints or disorders for at least 84 weeks in the database of the Dutch Social Security Institute. We performed univariable logistic regression analyses (p ≤ 0.157) for all independent variables with the dependent variable staying at work for the workers with SHC. We then performed multivariable logistic regression analyses with forward selection (p ≤ 0.157) and combined the remaining factors in a final, multivariable model (p ≤ 0.05), which we also used for logistic regression analysis in the workers with other disorders. The following factors were significant prognostic factors for staying at work for workers with SHC: full work disability benefits (odds ratio (OR) 0.07, 95% confidence interval (95% CI) 0.01–0.64), good mental health (OR 1.08, 95% CI 1.02–1.14), positive expectations for staying at work (OR 6.49, 95% CI 2.00–21.09), previous absenteeism for the same health complaint (OR 0.31, 95% CI 0.10–0.96) and good coping strategies (OR 1.13, 95% CI 1.04–1.23). For workers with other disorders, full work disability benefits, good mental health and positive expectations for staying at work were also prognostic factors for staying at work. Individual and policy factors seem to be important for staying at work of sick-listed workers with SHC and those with other disorders alike, but several biopsychosocial factors are particularly important for workers with SHC.
Hypoxic Pilates Intervention for Obesity: A Randomized Controlled Trial
Authors: Jung Kyounghwa , Kim Jisu , Park Hun-Young , Jung Won-Sang , Lim Kiwon
Published: October 2020

This study examined the effect of Pilates training under hypoxia, a novel treatment method, for obesity. Thirty-two Korean women with obesity (age: 34–60 (47.5 ± 7.5) years) were randomly assigned to control (CON; n = 10), normoxic Pilates training (NPTG; n = 10), and hypoxic Pilates training groups (HPTG; n = 12). The NPTG and HPTG performed 50 min of Pilates training using a tubing band for 12 weeks (3 days/week) in their respective environmental conditions (NPTG: normoxic condition, inspired oxygen fraction (FiO2) = 20.9%; HPTG: moderate hypoxic condition, FiO2 = 14.5%). The CON maintained their daily lifestyle without intervention. All subjects underwent body composition, blood pressure, arterial stiffness, vascular endothelial function, cardiometabolic biomarker, hemorheological function, and aerobic performance measurements before and after the intervention. The HPTG showed a significant improvement in diastolic blood pressure, total cholesterol and triglyceride concentrations, flow-mediated dilation, and erythrocyte deformability and aggregation (all p < 0.05) compared with the CON and NPTG. However, compared with the CON and NPTG, the HPTG did not show improvement in other parameters. Hypoxic Pilates intervention is a novel and successful method for promoting endothelial and hemorheological functions in women with obesity.
The Knowledge, Attitude, Practices, and Satisfaction of Non-Invasive Prenatal Testing among Chinese Pregnant Women under Different Payment Schemes: A Comparative Study
Authors: Zhu Wenjun , Ling XiaoXiao , Shang Wenru , Huang Jiayan
Published: October 2020

Non-invasive prenatal testing (NIPT) for aneuploidy screening has been widely applied across China, and costs can affect Chinese pregnant women’s choices. This study aims to assess the knowledge, attitude, practices (KAP) and satisfaction regarding NIPT among pregnant women in China, and to further explore the relationship between payment schemes and women’s acceptability of and satisfaction with NIPT. A questionnaire survey was performed in Shenzhen and Zhengzhou, China, which separately applied “insurance coverage” and “out-of-pocket” payment scheme for NIPT. The major differences between the two cities were compared using chi-square test, Wilcoxon rank sum test, and propensity score matched analysis. Logistic regression models were applied to explore predictors for women’s acceptability and satisfaction. Compared with Zhengzhou participants, a higher proportion of Shenzhen women had heard of NIPT (87.30% vs. 64.03%), were willing to receive NIPT (91.80% vs. 80.43%) and had taken NIPT (83.12% vs. 54.54%), while their satisfaction level was lower. Having NIPT-related knowledge was associated with higher acceptability, and receiving genetic counseling helped to improve satisfaction. Besides, women with higher annual household incomes were more likely to take and be satisfied with NIPT. In conclusion, more attention should be paid to health education, subsidies for NIPT, and genetic counseling.
What Drives Households’ Payment for Waste Disposal and Recycling Behaviours? Empirical Evidence from South Africa’s General Household Survey
Authors: Omotayo Abiodun Olusola , Omotoso Abeeb Babatunde , Daud Adebola Saidat , Ogunniyi Adebayo Isaiah , Olagunju Kehinde Oluseyi
Published: October 2020

Safeguarding the environment and its citizens’ health remains one of the key policy priorities of the governments of many developing and emerging countries. Using the 2017 General Household Survey (GHS) dataset, this study examines the driving factors affecting households’ recycling behaviour and payment for waste disposal in South Africa. The methods of data analysis were based on descriptive statistics and a Bivariate Probit regression model. The descriptive statistics results indicate that there are 56.29% male-headed and 43.71% female headed households, with an average age of 49 years. In addition, the study shows that 89.97% of household heads had formal education with a mean monthly income of 11,099.07 ZAR/650.504 USD. The study also revealed that 22% of the households sampled had access to social grants. The results from the Bivariate Probit regression model show that household’s income, access to social grants, formal educational attainment and the age of the household were significant (p < 0.01) driving factors affecting households’ recycling behaviour and payment for waste disposal. The study concludes that the households’ socio-economic factors affect their recycling behaviour and willingness to pay for waste management in South Africa. Actions targeted at poverty alleviation and environmental sensitization programmes are key for facilitating environmental conservation behaviours of households in South Africa in order to achieve the environmental sustainability Sustainable Development Goal (SDG) target of the United Nations.
Vaccine Hesitancy among Master’s Degree Students in Nursing and Midwifery: Attitude and Knowledge about Influenza Vaccination
Authors: Mellucci Claudia , Tamburrano Andrea , Cassano Fabiana , Galletti Caterina , Sguera Anna , Damiani Gianfranco , Laurenti Patrizia
Published: October 2020

Influenza vaccination among healthcare workers may reduce morbidity and protect fragile patients. Most of the evidence concerning the vaccine hesitancy of healthcare workers reported lack of knowledge and wrong attitude. The aims of this study were to explore the knowledge and attitudes about influenza vaccination among master’s degree students in Nursing and Midwifery, and to evaluate the effectiveness of their involvement in the hospital vaccination campaign in order to increase intention to receive immunization. The students of nurses and midwives were involved in the vaccination sessions of the 2018–19 hospital campaign. They were recruited to complete an online survey. Students of the 2nd year (involved in the vaccination campaign) and the 1st year (not involved) were compared. Descriptive and inferential statistics were performed for data analysis. Students who intend to receive influenza vaccination in the following year registered a percentage of 83.6% and showed an overall attitude of 66.8%. The involvement of the students in the vaccination campaign led to a significant increase in their positive vaccination attitude (80.9% vs. 87.0%) and in their intention to receive flu vaccination in the following year (67.7% vs. 100%). A positive attitude towards vaccinations was observed by nurses and midwives. Their involvement in the planning and activities during the vaccination campaign could positively influence their opinions and intention to receive vaccination.
The Influence of Thermal Alterations on Prefrontal Cortex Activation and Neuromuscular Function during a Fatiguing Task
Authors: Phillips Kevin Cyle , Verbrigghe Derek , Gabe Alex , Jauquet Brittany , Eischer Claire , Yoon Tejin
Published: October 2020

The purpose of this study was to examine prefrontal cortex (PFC) activation, neuromuscular function, and perceptual measures in response to a fatiguing task, following thermal alterations of an exercising arm. Nineteen healthy adults completed three experimental sessions. At baseline, participants performed maximum voluntary isometric contractions (MVIC) of the elbow flexors. Next, participants submerged their right arm in a water bath for 15 min. Cold (C), neutral (N), and hot (H) water temperatures were maintained at 8, 33, and 44 °C, respectively. Following water immersion, participants performed an isometric elbow flexion contraction, at 20% of their MVIC, for 5 min. Ratings of perceived exertion (RPE), muscular discomfort, and task demands were assessed. Functional near-infrared spectroscopy was used to measure activation (oxygenation) of the PFC during the fatiguing task. Reductions in MVIC torque at the end of the fatiguing task were greater for the H (25.7 ± 8.4%) and N (22.2 ± 9.6%) conditions, compared to the C condition (17.5 ± 8.9%, p < 0.05). The increase in oxygenation of the PFC was greater for the H (13.3 ± 4.9 μmol/L) and N (12.4 ± 4.4 μmol/L) conditions, compared to the C condition (10.3 ± 3.8 μmol/L, p < 0.001) at the end of the fatiguing task. The increase in RPE, muscular discomfort, and task demands were greater in the H condition compared to the N and C conditions (p < 0.01). These results indicate that precooling an exercising arm attenuates the rise in PFC activation, muscle fatigue, and psychological rating during a fatiguing task.
The Association between Insulin Resistance and Cardiovascular Disease Risk: A Community-Based Cross-Sectional Study among Taiwanese People Aged over 50 Years
Authors: Lu Mei-Chun , Fang Wei-Ching , Li Wen-Cheng , Yeh Wei-Chung , Shieh Ying-Hua , Chen Jau-Yuan
Published: October 2020

Background and Aims: Previous studies have implied that insulin resistance (IR) could represent a major underlying abnormality leading to cardiovascular disease (CVD). The aim of this study was to evaluate the relationships between IR (estimated by the homeostasis model assessment of IR (HOMA-IR) index) and CVD risk among middle-aged and elderly Taiwanese individuals. Methods: In this cross-sectional, community-based study, a total of 320 participants were interviewed to collect demographical parameters and blood samples. The recruited participants were divided into tertiles according to their levels of HOMA-IR. The Framingham risk score (FRS) was calculated according to the 2008 general CVD risk model from the Framingham Heart Study. Results: The HOMA-IR index was significantly correlated with the FRS, with a Pearson’s coefficient of 0.22. In the multiple logistic regression model, a higher HOMA-IR level was significantly associated with a high FRS (FRS ≥ 20%) (highest tertile vs. lowest tertile of HOMA-IR, crude OR = 3.69; 95% CI = 1.79–7.62), even after adjusting for smoking, fasting plasma glucose (FPG), and systolic blood pressure (SBP) (highest tertile vs. lowest tertile of HOMA-IR, adjusted OR = 11.51; 95% CI = 2.55–51.94). The area under the receiver operating characteristic curve for the HOMA-IR index as the predictor of high FRS was 0.627, and the optimal HOMA-IR cutoff value was 1.215 (sensitivity = 83.6%, specificity = 42.9%). Conclusions: We considered that HOMA-IR is an independent factor but that it cannot be used solely for evaluating the CVD risk due to the low AUC value. Further prospective cohort studies are warranted to better assess the relationship between CVD risk and insulin resistance.
Health Behaviors and Health Status among Middle-Aged and Older Adults with Chronic Diseases in Taiwan
Authors: Tian Wei-Hua , Tien Joseph J.
Published: October 2020

Changes in lifestyle behaviors may effectively maintain or improve the health status of individuals with chronic diseases. However, such health behaviors adopted by individuals are unlikely to demonstrate similar patterns. This study analyzed the relationship between the heterogeneous latent classes of health behavior and health statuses among middle-aged and older adults with hypertension, diabetes, or hyperlipidemia in Taiwan. After selecting 2103 individuals from the 2005 and 2009 Taiwan National Health Interview Survey (NHIS), we first identified heterogeneous groups of health behaviors through latent class analysis (LCA). We further explored the relationship between each latent class of health behavior and health status through ordered logit regression. We identified the following five distinct health behavior classes: the all-controlled, exercise and relaxation, healthy diet and reduced smoking or drinking, healthy diet, and least-controlled classes. Regression results indicated that individuals in classes other than the all-controlled class all reported poor health statuses. We also found great magnitude of the coefficient estimates for individuals who reported their health status to be poor or very poor for the least-controlled class. Therefore, health authorities and medical providers may develop targeted policies and interventions that address multiple modifiable health behaviors in each distinct latent class of health behavior.
Developing a Family-Centered Care Model in the Neonatal Intensive Care Unit (NICU): A New Vision to Manage Healthcare
Authors: Gómez-Cantarino Sagrario , García-Valdivieso Inmaculada , Moncunill-Martínez Eva , Yáñez-Araque Benito , Ugarte Gurrutxaga M. Idoia
Published: October 2020

Family-centered care (FCC) currently takes a greater role in health care, due to the increasing empowerment parents experience. Within neonatal intensive care units (NICUs), family participation has an impact on the humanized care of the preterm newborn (PN). This integrative review conducted according to Whittemore and Knafl investigated current knowledge of the FCC model and its application in PN care in specific units. The data were collected from PubMed, Cochrane, CINHAL, Scopus, and Google Scholar. A total of 45 articles were used, of which 13 were selected which met inclusion criteria. Their methodological quality was evaluated using the mixed method appraisal tool (MMAT), and after they were analyzed and grouped into four thematic blocks: (1) parental participation; (2) health parental training; (3) benefits of family empowerment; and (4) humanized care. The results revealed that FCCs promote the integration of health equipment and family. In addition, parents become the primary caregivers. The benefits of the family–PN binomial enable an earlier hospital discharge. Humanized care involves an ethical approach, improving health care. Changes are still needed by health managers to adapt health services to the needs of the family and PNs.
Distal Displacement of Maxillary Sinus Anterior Wall Versus Conventional Sinus Lift with Lateral Access: A 3-Year Retrospective Computerized Tomography Study
Authors: Menchini-Fabris Giovanni Battista , Toti Paolo , Crespi Giovanni , Covani Ugo , Crespi Roberto
Published: October 2020

Background: The present study is designed to compare the outcomes of two sinus augmentation procedures: distal displacement of the anterior wall versus standard sinus lifting and grafting with a lateral window approach. Methods: In the displacement group, a localized surgical fracture of the sinus floor achieved through an electromagnetic device results in the distal displacement of the anterior wall. In the filling group, sinus lifting (with lateral access) and grafting with particulate xenogeneic bone substitute was performed. Bone volume beneath the maxillary sinus was investigated with computerized tomography after baseline and postoperative data superimposition. Clinical and radiological outcomes over three years had been evaluated. Results: Forty-three dental implants were selected. The two sinus lift procedures significantly increased the bone volume (p-value ≤ 0.0017) in the displacement group from 1.17 ± 0.34 to 1.53 ± 0.39 cc, with a final bone gain of +0.36 ± 0.17 cc, and in the filling group from 1.24 ± 0.41 to 1.94 ± 0.68 cc, with a bone augmentation of +0.71 ± 0.31 cc. No events of dental implant bulging into the maxillary sinus occurred. Two implants failed early on in the filling group, attesting the 3-year survival rate of 92.6% (CI95%: 82.7–100%). Marginal bone loss at the distal aspect was 1.66 ± 0.72 and 1.25 ± 0.78 mm, respectively, for the displacement and filling groups, with a significant difference (p-value = 0.0497). Conclusion: Results showed a significant and effective bone gain around dental implants at a 3-year survey for both sinus augmented by backward displacement of the anterior wall (+34%) and sinus lifting and grafting with a lateral window approach (+57%).
Mutualistic Pattern of Intra-Urban Agglomeration and Impact Analysis: A Case Study of 11 Urban Agglomerations of Mainland China
Authors: Tian Yasi
Published: September 2020

Despite the worldwide studies on urban agglomeration (UA), the effects of intra-UA interaction patterns have not been thoroughly elucidated to date. To fill the research gap, first, this study utilized the Baidu Internet search data to quantify the internal interaction patterns of 11 main UAs in China. Rail-way data were referenced for verification. Based on building intercity interaction network, the node symmetry index (NSI) was calculated. Considering the estimated interaction strength and mutuality, the intra-interaction patterns were classified into symmetrical and asymmetrical mutualism, where the former indicates that the interactions of cities are mutually beneficial and the latter means that the interactions are unbalanced. The socio-economic development levels of cities and UAs were estimated by the entropy-TOPSIS (Technique for Order Preference by Similarity to an Ideal Solution) method. Finally, the impacts of intra-UA interaction were explored through ordinary least square regression. This study obtained two findings. Firstly, at the city scale, symmetrical mutualism had a greater impact than asymmetrical mutualism on the city’s socio-economic development level. Secondly, at the regional scale, both symmetrical and asymmetrical mutualism were related with regional socioeconomic development level; however, only symmetrical mutualism showed a correlation with regional coordinated development level. Respondent suggestions and implications to promote regional coordinated development were then offered based on the results of the analysis. Limitations of this study include that exogenous interactions between UAs and their backlands, and other relationships, such as competition, were not discussed. These issues can be considered in future researches. This study characterizes the interaction pattern of intra-urban agglomeration and offers advice and suggestion for implementing regional sustainable development.
Disparities in Geographical Access to Hospitals in Portugal
Authors: Costa Claudia , Tenedório José António , Santana Paula
Published: September 2020

Geographical accessibility to health care services is widely accepted as relevant to improve population health. However, measuring it is very complex, mainly when applied at administrative levels that go beyond the small-area level. This is the case in Portugal, where the municipality is the administrative level that is most appropriate for implementing policies to improve the access to those services. The aim of this paper is to assess whether inequalities in terms of access to a hospital in Portugal have improved over the last 20 years. A population-weighted driving time was applied using the census tract population, the roads network, the reference hospitals’ catchment area and the municipality boundaries. The results show that municipalities are 25 min away from the hospital—3 min less than in 1991—and that there is an association with premature mortality, elderly population and population density. However, disparities between municipalities are still huge. Municipalities with higher rates of older populations, isolated communities or those located closer to the border with Spain face harder challenges and require greater attention from local administration. Since municipalities now have responsibilities for health, it is important they implement interventions at the local level to tackle disparities impacting access to healthcare.
Histone-Mutant Glioma: Molecular Mechanisms, Preclinical Models, and Implications for Therapy
Authors: Graham Maya S. , Mellinghoff Ingo K.
Published: September 2020

Pediatric high-grade glioma (pHGG) is the leading cause of cancer death in children. Despite histologic similarities, it has recently become apparent that this disease is molecularly distinct from its adult counterpart. Specific hallmark oncogenic histone mutations within pediatric malignant gliomas divide these tumors into subgroups with different neuroanatomic and chronologic predilections. In this review, we will summarize the characteristic molecular alterations of pediatric high-grade gliomas, with a focus on how preclinical models of these alterations have furthered our understanding of their oncogenicity as well as their potential impact on developing targeted therapies for this devastating disease.
Enhanced Internalization of Nanoparticles Following Ionizing Radiation Leads to Mitotic Catastrophe in MG-63 Human Osteosarcoma Cells
Authors: Popescu Roxana Cristina , Straticiuc Mihai , Mustăciosu Cosmin , Temelie Mihaela , Trușcă Roxana , Vasile Bogdan Ștefan , Boldeiu Adina , Mirea Dragoş , Andrei Radu Florin , Cenușă Constantin , Mogoantă Laurenţiu , Mogoșanu George Dan , Andronescu Ecaterina , Radu Mihai , Veldwijk Marlon R. , Savu Diana Iulia
Published: September 2020

This study aims to investigate whether ionizing radiation combined with doxorubicin-conjugated iron oxide nanoparticles (NP-DOX) improves the internalization and cytotoxic effects of the nano-carrier-mediated drug delivery in MG-63 human osteosarcoma cells. NP-DOX was designed and synthesized using the co-precipitation method. Highly stable and crystalline nanoparticles conjugated with DOX were internalized in MG-63 cells through macropinocytosis and located in the perinuclear area. Higher nanoparticles internalization in MG-63 cells previously exposed to 1 Gy X-rays was correlated with an early accumulation of cells in G2/M, starting at 12 h after treatment. After 48 h, the application of the combined treatment led to higher cytotoxic effects compared to the individual treatment, with a reduction in the metabolic capacity and unrepaired DNA breaks, whilst a low percent of arrested cells, contributing to the commitment of mitotic catastrophe. NP-DOX showed hemocompatibility and no systemic cytotoxicity, nor histopathological alteration of the main organs.
Preventive Effects of Quercetin against the Onset of Atherosclerosis-Related Acute Aortic Syndromes in Mice
Authors: Kondo Masateru , Izawa-Ishizawa Yuki , Goda Mitsuhiro , Hosooka Mayuko , Kagimoto Yuu , Saito Naoko , Matsuoka Rie , Zamami Yoshito , Chuma Masayuki , Yagi Kenta , Takechi Kenshi , Tsuneyama Koichi , Ishizawa Keisuke
Published: September 2020

Atherosclerosis-related acute aortic syndromes, such as aortic aneurysms or aortic dissection are life-threatening diseases. Since they develop suddenly and progress rapidly, the establishment of preventive strategies is urgently needed. Quercetin, a flavonoid abundant in various vegetables and fruits, is suggested to reduce the risk of cardiovascular disease. Therefore, in this study, the preventive effect of quercetin was evaluated using a mouse model of aortic aneurysm and dissection. The model was established by administering angiotensin II (Ang II) and β-aminopropionitrile (BAPN), a lysyl oxidase inhibitor, to mice to induce hypertension and degeneration of the elastic lamina, which would eventually result in the onset of an aortic aneurysm. Ang II, BAPN, and a nitric oxide synthase inhibitor was administered to induce aortic dissection via endothelial dysfunction. Quercetin (60 mg/kg/day) was administered 2 weeks before inducing aortic diseases by the end of the experiments (8 weeks in the aneurysm model, 6 weeks in the dissection model). It was found to reduce the incidence of aneurysm (from 72 to 45%), dissection (from 17 to 10%), and rupture (from 33 to 15%) in mice. Elastin degradation was ameliorated in the quercetin-treated mice compared to that in the mice without quercetin treatment (degradation score 2.9 ± 0.3 vs 2.2 ± 0.2). Furthermore, quercetin suppressed the expression of vascular cell adhesion molecule-1, macrophage infiltration, and pro-matrix metalloproteinase-9 activity. Our results suggest that quercetin might prevent the onset of atherosclerosis-related acute aortic syndromes through its anti-inflammatory and endothelial cell-protective effects.
Astaxanthin Suppresses PM2.5-Induced Neuroinflammation by Regulating Akt Phosphorylation in BV-2 Microglial Cells
Authors: Kim Ryeong-Eun , Shin Chan Young , Han Seol-Heui , Kwon Kyoung Ja
Published: October 2020

Air pollution has become one of the most serious issues for human health and has been shown to be particularly concerning for neural and cognitive health. Recent studies suggest that fine particulate matter of less than 2.5 (PM2.5), common in air pollution, can reach the brain, potentially resulting in the development and acceleration of various neurological disorders including Alzheimer’s disease, Parkinson’s disease, and other forms of dementia, but the underlying pathological mechanisms are not clear. Astaxanthin is a red-colored phytonutrient carotenoid that has been known for anti-inflammatory and neuroprotective effects. In this study, we demonstrated that exposure to PM2.5 increases the neuroinflammation, the expression of proinflammatory M1, and disease-associated microglia (DAM) signature markers in microglial cells, and that treatment with astaxanthin can prevent the neurotoxic effects of this exposure through anti-inflammatory properties. Diesel particulate matter (Sigma-Aldrich) was used as a fine particulate matter 2.5 in the present study. Cultured rat glial cells and BV-2 microglial cells were treated with various concentrations of PM2.5, and then the expression of various inflammatory mediators and signaling pathways were measured using qRT-PCR and Western blot. Astaxanthin was then added and assayed as above to evaluate its effects on microglial changes, inflammation, and toxicity induced by PM2.5. PM2.5 increased the production of nitric oxide and reactive oxygen species and upregulated the transcription of various proinflammatory markers including Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Tumor necrosis factor α (TNFα), inducible nitric oxide synthase (iNOS), triggering receptor expressed on myeloid cells 2 (TREM2), Toll-like receptor 2/4 (TLR2/4), and cyclooxygenase-2 (COX-2) in BV-2 microglial cells. However, the mRNA expression of IL-10 and arginase-1 decreased following PM2.5 treatment. PM2.5 treatment increased c-Jun N-terminal kinases (JNK) phosphorylation and decreased Akt phosphorylation. Astaxanthin attenuated these PM2.5-induced responses, reducing transcription of the proinflammatory markers iNOS and heme oxygenase-1 (HO-1), which prevented neuronal cell death. Our results indicate that PM2.5 exposure reformulates microglia via proinflammatory M1 and DAM phenotype, leading to neurotoxicity, and the fact that astaxanthin treatment can prevent neurotoxicity by inhibiting transition to the proinflammatory M1 and DAM phenotypes. These results demonstrate that PM2.5 exposure can induce brain damage through the change of proinflammatory M1 and DAM signatures in the microglial cells, as well as the fact that astaxanthin can have a potential beneficial effect on PM2.5 exposure of the brain.
Glucose, Fructose, and Urate Transporters in the Choroid Plexus Epithelium
Authors: Chiba Yoichi , Murakami Ryuta , Matsumoto Koichi , Wakamatsu Keiji , Nonaka Wakako , Uemura Naoya , Yanase Ken , Kamada Masaki , Ueno Masaki
Published: October 2020

The choroid plexus plays a central role in the regulation of the microenvironment of the central nervous system by secreting the majority of the cerebrospinal fluid and controlling its composition, despite that it only represents approximately 1% of the total brain weight. In addition to a variety of transporter and channel proteins for solutes and water, the choroid plexus epithelial cells are equipped with glucose, fructose, and urate transporters that are used as energy sources or antioxidative neuroprotective substrates. This review focuses on the recent advances in the understanding of the transporters of the SLC2A and SLC5A families (GLUT1, SGLT2, GLUT5, GLUT8, and GLUT9), as well as on the urate-transporting URAT1 and BCRP/ABCG2, which are expressed in choroid plexus epithelial cells. The glucose, fructose, and urate transporters repertoire in the choroid plexus epithelium share similar features with the renal proximal tubular epithelium, although some of these transporters exhibit inversely polarized submembrane localization. Since choroid plexus epithelial cells have high energy demands for proper functioning, a decline in the expression and function of these transporters can contribute to the process of age-associated brain impairment and pathophysiology of neurodegenerative diseases.
Identifying Stabilin-1 and Stabilin-2 Double Knockouts in Reproduction and Placentation: A Descriptive Study
Authors: Kim Soon-Young , Lee Eun-Hye , Kim Eun Na , Son Woo-Chan , Kim Yeo Hyang , Park Seung-Yoon , Kim In-San , Kim Jung-Eun
Published: October 2020

The placenta undergoes reconstruction at different times during fetal development to supply oxygen and nutrients required throughout pregnancy. To accommodate the rapid growth of the fetus, small spiral arteries undergo remodeling in the placenta. This remodeling includes apoptosis of endothelial cells that line spiral arteries, which are replaced by trophoblasts of fetal origin. Removal of dead cells is critical during this process. Stabilin-1 (Stab1) and stabilin-2 (Stab2) are important receptors expressed on scavenger cells that absorb and degrade apoptotic cells, and Stab1 is expressed in specific cells of the placenta. However, the role of Stab1 and Stab2 in placental development and maintenance remain unclear. In this study, we assessed Stab1 and Stab2 expression in the placenta and examined the reproductive capacity and placental development using a double-knockout mouse strain lacking both Stab1 and Stab2 (Stab1/2 dKO mice). Most pregnant Stab1/2 dKO female mice did not produce offspring and exhibited placental defects, including decidual hemorrhage and necrosis. Findings of this study offer the first description of the phenotypic characteristics of placentas and embryos of Stab1/2 dKO females during pregnancy, suggesting that Stab1 and Stab2 are involved in placental development and maintenance.
Characterization of Local and Systemic Impact of Whitefly (Bemisia tabaci) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics
Authors: Ogden Aaron J. , Boukari Wardatou , Nava Alba , Lucinda Natalia , Sunter Garry , Curtis Wayne R. , Adkins Joshua N. , Polston Jane E.
Published: October 2020

Tomato mottle virus (ToMoV) is a single-stranded DNA (ssDNA) begomovirus transmitted to solanaceous crops by the whitefly species complex (Bemisia tabaci), causing stunted growth, leaf mottling, and reduced yield. Using a genetic repertoire of seven genes, ToMoV pathogenesis includes the manipulation of multiple plant biological processes to circumvent antiviral defenses. To further understand the effects of whitefly feeding and whitefly-transmitted ToMoV infection on tomato plants (Solanum lycopersicum ‘Florida Lanai’), we generated comprehensive protein profiles of leaves subjected to feeding by either viruliferous whiteflies harboring ToMoV, or non-viruliferous whiteflies, or a no-feeding control. The effects of whitefly feeding and ToMoV infection were measured both locally and systemically by sampling either a mature leaf directly from the site of clip-cage confined whitefly feeding, or from a newly formed leaf 10 days post feeding (dpf). At 3 dpf, tomato’s response to ToMoV included proteins associated with translation initiation and elongation as well as plasmodesmata dynamics. In contrast, systemic impacts of ToMoV on younger leaves 10 dpf were more pronounced and included a virus-specific change in plant proteins associated with mRNA maturation and export, RNA-dependent DNA methylation, and other antiviral plant processes. Our analysis supports previous findings and provides novel insight into tomato’s local and systemic response to whitefly feeding and ToMoV infection.