bluuebunny/crossref_metadata_2025 huggingface dataset:
Improvement of Cutaneous Wound Healing via Topical Application of Heat-Killed Lactococcus chungangensis CAU 1447 on Diabetic Mice
Authors: Nam Yohan , Kim Jonghwa , Baek Jihye , Kim Wonyong
Published: August 2021

Cutaneous wound healing comprises a complex systemic network. Probiotics, naturally extracted substances, medicine, and chemical compounds have been used for wound healing, but the application of postbiotics as therapeutic agents has yet to be explored. Our study shows potential beneficial effects of heat-killed Lactococcus chungangensis CAU 1447 on type 1 diabetic mice. The postbiotic strain significantly decreased the skin wound size. The activity of myeloperoxidase secreted from neutrophils also decreased. The molecular mechanism of wound healing was adjusted by important mediators, growth factors, chemokines, and cytokines. These elements regulated the anti-inflammatory activity and accelerated wound healing. To determine the role of the postbiotic in wound repair, we showed a similar taxonomic pattern as compared to the diabetic mice using skin microbiome analysis. These findings demonstrated that heat-killed Lactococcus chungangensis CAU 1447 had beneficial effects on wound healing and can be utilized as postbiotic therapeutic agents.
Gut Microbiota Modulation in the Context of Immune-Related Aspects of Lactobacillus spp. and Bifidobacterium spp. in Gastrointestinal Cancers
Authors: Kaźmierczak-Siedlecka Karolina , Roviello Giandomenico , Catalano Martina , Polom Karol
Published: August 2021

Accumulating evidence has revealed the critical roles of commensal microbes in cancer progression and recently several investigators have evaluated the therapeutic effectiveness of targeting the microbiota. This gut microbiota-related approach is especially attractive in the treatment of gastrointestinal cancers. Probiotics supplementation is a microbiota-targeted strategy that appears to improve treatment efficacy; Lactobacillus spp. and Bifidobacterium spp. are among the most commonly used probiotic agents. These bacteria seem to exert immunomodulatory effects, impacting on the immune system both locally and systemically. The gut microbiota are able to affect the efficiency of immunotherapy, mainly acting as inhibitors at immune checkpoints. The effects of immunotherapy may be modulated using traditional probiotic strains and/or next generation probiotics, such as Akkermansia municiphila. It is possible that probiotics might enhance the efficiency of immunotherapy based on PD-1/PD-L1 and CTLA-4 but more data are needed to confirm this speculation. Indeed, although there is experimental evidence for the efficacy of several strains, the health-promoting effects of numerous probiotics have not been demonstrated in human patients and furthermore the potential risks of these products, particularly in oncologic patients, are rarely mentioned.
Special Issue “Mediterranean Diet and Metabolic Diseases”
Authors: Magriplis Emmanuella , Chourdakis Michail
Published: August 2021

The Mediterranean diet (MD) has been considered among the healthiest dietary patterns since a little over 50 years ago, Ancel Keys—as the key figure—provided evidence for the beneficial effects of the MD [...]
Adjuvant Effect of Orally Applied Preparations Containing Non-Digestible Polysaccharides on Influenza Vaccination in Healthy Seniors: A Double-Blind, Randomised, Controlled Pilot Trial
Authors: Laue Christiane , Stevens Yala , van Erp Monique , Papazova Ekaterina , Soeth Edlyn , Pannenbeckers Angelika , Stolte Ellen , Böhm Ruwen , Gall Sophie Le , Falourd Xavier , Ballance Simon , Knutsen Svein H. , Pinheiro Iris , Possemiers Sam , Ryan Paul M. , Ross R. Paul , Stanton Catherine , Wells Jerry M. , Werf Sylvie van der , Mes Jurriaan J. , Schrezenmeir Juergen
Published: August 2021

Senior individuals can suffer from immunosenescence and novel strategies to bolster the immune response could contribute to healthy ageing. In this double-blind, randomised, controlled pilot trial, we investigated the ability of non-digestible polysaccharide (NPS) preparations to enhance the immune response in a human vaccination model. In total, 239 subjects (aged 50–79 years) were randomised to consume one of five different NPS (yeast β-glucan (YBG), shiitake β-glucan (SBG), oat β-glucan (OBG), arabinoxylan (AX), bacterial exopolysaccharide (EPS)) or control (CTRL) product daily for five weeks. After two weeks of intervention, subjects were vaccinated with seasonal influenza vaccine. The post-vaccination increases in haemagglutination inhibition antibody titres and seroprotection rate against the influenza strains were non-significantly enhanced in the NPS intervention groups compared to CTRL. Specifically, a trend towards a higher mean log2 fold increase was observed in the AX group (uncorrected p = 0.074) combined with a trend for an increased seroprotection rate, AX group (48.7%) compared to CTRL (25.6%) (uncorrected p = 0.057), for the influenza A H1N1 strain. Subjects consuming AX also had a reduced incidence of common colds compared to CTRL (1 vs. 8; p = 0.029 in Fisher exact test). No adverse effects of NPS consumption were reported. The findings of this pilot study warrant further research to study AX as an oral adjuvant to support vaccine efficacy.
Discharge Plan to Promote Patient Safety and Shared Decision Making by a Multidisciplinary Team of Healthcare Professionals in a Respiratory Unit
Authors: Nnate Daniel A. , Barber David , Abaraogu Ukachukwu O.
Published: August 2021

Patients with chronic obstructive pulmonary disease (COPD) often require frequent hospitalization due to worsening symptoms. Preventing prolonged hospital stays and readmission becomes a challenge for healthcare professionals treating patients with COPD. Although the integration of health and social care supports greater collaboration and enhanced patient care, organizational structure and poor leadership may hinder the implementation of patient-oriented goals. This paper presents a case of a 64-year-old chronic smoker with severe COPD who was to be discharged on long-term oxygen therapy (LTOT). It also highlights the healthcare decisions made to ensure the patient’s safety at home and further provides a long-lasting solution to the existing medical and social needs. The goal was accomplished through a discharge plan that reflects multidisciplinary working, efficient leadership, and change management using Havelock’s theory. While COPD is characterized by frequent exacerbation and hospital readmission, it was emphasized that most failed discharges could be attributed to bureaucratic organizational workflow which might not be in the patient’s best interest. It was further demonstrated that healthcare professionals are likely to miss the window of opportunity to apply innovative and long-lasting solutions to the patient’s health condition in an attempt to remedy the immediate symptoms of COPD.
Detection and Characterization of Clinical Bordetella trematum Isolates from Chronic Wounds
Authors: Buechler Christian , Neidhöfer Claudio , Hornung Thorsten , Neuenhoff Marcel , Parčina Marijo
Published: August 2021

Bordetella trematum is a relatively newly discovered and potentially frequently overlooked Bordetella species, mostly isolated from chronic wounds and predominantly in those of the lower extremities. Its susceptibility profile and clinical significance is still debated, given the limited amount of available data. We contribute providing a molecular and phenotypical analysis of three unique clinical B. trematum isolates detected between August 2019 and January 2020 to aid the matter. Cryo-conserved isolates were subcultured and re-identified using various routine means of identification. Bacterial genomes were fully Illumina-sequenced and phenotypical susceptibility was determined by broth microdilution and gradient-strip tests. All isolates displayed increased susceptibility to piperacillin–tazobactam (<2/4 mg/L), imipenem (<1 mg/L), and meropenem (<0.047 mg/L), whereas they displayed decreased susceptibility to all tested cephalosporins and fluoroquinolones (according to PK-PD, EUCAST 10.0 2020). One isolate carried a beta-lactamase (EC 3.5.2.6) and a sulfonamide resistance gene (sul2) and cells displayed resistance to ampicillin, ampicillin/sulbactam, and trimethoprim/sulfamethoxazole. All isolates carried genes conferring decreased susceptibility to aminoglycosides (aadA), fosfomycin (fosA) and fluoroquinolones (gyrB EC 5.99.1.3). Awareness that B. trematum can be resistant to trimethoprim/sulfamethoxazole is warranted.
Monocyte Infiltration and Differentiation in 3D Multicellular Spheroid Cancer Models
Authors: Madsen Natasha Helleberg , Nielsen Boye Schnack , Nhat Son Ly , Skov Søren , Gad Monika , Larsen Jesper
Published: August 2021

Tumor-associated macrophages often correlate with tumor progression, and therapies targeting immune cells in tumors have emerged as promising treatments. To select effective therapies, we established an in vitro 3D multicellular spheroid model including cancer cells, fibroblasts, and monocytes. We analyzed monocyte infiltration and differentiation in spheroids generated from fibroblasts and either of the cancer cell lines MCF-7, HT-29, PANC-1, or MIA PaCa-2. Monocytes rapidly infiltrated spheroids and differentiated into mature macrophages with diverse phenotypes in a cancer cell line-dependent manner. MIA PaCa-2 spheroids polarized infiltrating monocytes to M2-like macrophages with high CD206 and CD14 expression, whereas monocytes polarized by MCF-7 spheroids displayed an M1-like phenotype. Monocytes in HT-29 and PANC-1 primarily obtained an M2-like phenotype but also showed upregulation of M1 markers. Analysis of the secretion of 43 soluble factors demonstrated that the cytokine profile between spheroid cultures differed considerably depending on the cancer cell line. Secretion of most of the cytokines increased upon the addition of monocytes resulting in a more inflammatory and pro-tumorigenic environment. These multicellular spheroids can be used to recapitulate the tumor microenvironment and the phenotype of tumor-associated macrophages in vitro and provide more realistic 3D cancer models allowing the in vitro screening of immunotherapeutic compounds.
The Structural Biology of Eastern Equine Encephalitis Virus, an Emerging Viral Threat
Authors: Hasan S. Saif , Dey Debajit , Singh Suruchi , Martin Matthew
Published: August 2021

Alphaviruses are arboviruses that cause arthritis and encephalitis in humans. Eastern Equine Encephalitis Virus (EEEV) is a mosquito-transmitted alphavirus that is implicated in severe encephalitis in humans with high mortality. However, limited insights are available into the fundamental biology of EEEV and residue-level details of its interactions with host proteins. In recent years, outbreaks of EEEV have been reported mainly in the United States, raising concerns about public safety. This review article summarizes recent advances in the structural biology of EEEV based mainly on single-particle cryogenic electron microscopy (cryoEM) structures. Together with functional analyses of EEEV and related alphaviruses, these structural investigations provide clues to how EEEV interacts with host proteins, which may open avenues for the development of therapeutics.
Responses to Ecopollutants and Pathogenization Risks of Saprotrophic Rhodococcus Species
Authors: Ivshina Irina B. , Kuyukina Maria S. , Krivoruchko Anastasiia V. , Tyumina Elena A.
Published: August 2021

Under conditions of increasing environmental pollution, true saprophytes are capable of changing their survival strategies and demonstrating certain pathogenicity factors. Actinobacteria of the genus Rhodococcus, typical soil and aquatic biotope inhabitants, are characterized by high ecological plasticity and a wide range of oxidized organic substrates, including hydrocarbons and their derivatives. Their cell adaptations, such as the ability of adhering and colonizing surfaces, a complex life cycle, formation of resting cells and capsule-like structures, diauxotrophy, and a rigid cell wall, developed against the negative effects of anthropogenic pollutants are discussed and the risks of possible pathogenization of free-living saprotrophic Rhodococcus species are proposed. Due to universal adaptation features, Rhodococcus species are among the candidates, if further anthropogenic pressure increases, to move into the group of potentially pathogenic organisms with “unprofessional” parasitism, and to join an expanding list of infectious agents as facultative or occasional parasites.
Kynurenic Acid Accelerates Healing of Corneal Epithelium In Vitro and In Vivo
Authors: Matysik-Woźniak Anna , Turski Waldemar A. , Turska Monika , Paduch Roman , Łańcut Mirosław , Piwowarczyk Paweł , Czuczwar Mirosław , Rejdak Robert
Published: August 2021

Kynurenic acid (KYNA) is an endogenous compound with a multidirectional effect. It possesses antiapoptotic, anti-inflammatory, and antioxidative properties that may be beneficial in the treatment of corneal injuries. Moreover, KYNA has been used successfully to improve the healing outcome of skin wounds. The aim of the present study is to evaluate the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. Normal human corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) lines were used. Cellular metabolism, cell viability, transwell migration, and the secretion of IL-1β, IL-6, and IL-10 were determined. In rabbits, after corneal de-epithelization, eye drops containing 0.002% and 1% KYNA were applied five times a day until full recovery. KYNA decreased metabolism but did not affect the proliferation of the corneal epithelium. It decreased both the metabolism and proliferation of conjunctival epithelium. KYNA enhanced the migration of corneal but not conjunctival epithelial cells. KYNA reduced the secretion of IL-1β and IL-6 from the corneal epithelium, leaving IL-10 secretion unaffected. The release of all studied cytokines from the conjunctival epithelium exposed to KYNA was unchanged. KYNA at higher concentration accelerated the healing of the corneal epithelium. These favorable properties of KYNA suggest that KYNA containing topical pharmaceutical products can be used in the treatment of ocular surface diseases.
In Vitro Effect of Photodynamic Therapy with Different Lights and Combined or Uncombined with Chlorhexidine on Candida spp.
Authors: Pérez-Laguna Vanesa , Barrena-López Yolanda , Gilaberte Yolanda , Rezusta Antonio
Published: August 2021

Candidiasis is very common and complicated to treat in some cases due to increased resistance to antifungals. Antimicrobial photodynamic therapy (aPDT) is a promising alternative treatment. It is based on the principle that light of a specific wavelength activates a photosensitizer molecule resulting in the generation of reactive oxygen species that are able to kill pathogens. The aim here is the in vitro photoinactivation of three strains of Candida spp., Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258, using aPDT with different sources of irradiation and the photosensitizer methylene blue (MB), alone or in combination with chlorhexidine (CHX). Irradiation was carried out at a fluence of 18 J/cm2 with a light-emitting diode (LED) lamp emitting in red (625 nm) or a white metal halide lamp (WMH) that emits at broad-spectrum white light (420–700 nm). After the photodynamic treatment, the antimicrobial effect is evaluated by counting colony forming units (CFU). MB-aPDT produces a 6 log10 reduction in the number of CFU/100 μL of Candida spp., and the combination with CHX enhances the effect of photoinactivation (effect achieved with lower concentration of MB). Both lamps have similar efficiencies, but the WMH lamp is slightly more efficient. This work opens the doors to a possible clinical application of the combination for resistant or persistent forms of Candida infections.
Noise-Immune Labels of Residual Codes for Improving Solution Efficiency to Packet Overflow in an Optical Label-Switched Buffer
Authors: Chen Kai-Sheng , Yang Chao-Chin
Published: August 2021

In this paper, an optical buffering solution based on label switching is proposed, where packets are buffered by identifying and renewing the light labels of pseudo-orthogonal codes. The buffer overflow occurs when label switching fails to perform on the queued packets due to the insufficient labels. Assigning an increased code number to the buffer could reduce the overflow effect, but the decoder noise mitigates its efficiency. Therefore, we study a noise-immune labeling method of residual function by advancing the correlation properties of the existing codes. The proposed label-switching scheme improves the solution efficiency to buffer overflow as a lower code-error probability can be reached. Moreover, multiple label codes can be simultaneously generated from a shared light source to achieve a power-efficient buffer structure.
Chemical Composition and Antimicrobial Properties of Mentha × piperita cv. ‘Kristinka’ Essential Oil
Authors: Camele Ippolito , Gruľová Daniela , Elshafie Hazem S.
Published: August 2021

Several economically important crops, fruits and vegetables are susceptible to infection by pathogenic fungi and/or bacteria postharvest or in field. Recently, plant essential oils (EOs) extracted from different medicinal and officinal plants have had promising antimicrobial effects against phytopathogens. In the present study, the potential microbicide activity of Mentha × piperita cv. ‘Kristinka’ (peppermint) EO and its main constituents have been evaluated against some common phytopathogens. In addition, the cell membrane permeability of the tested fungi and the minimum fungicidal concentrations were measured. The antifungal activity was tested against the following postharvest fungi: Botrytis cinerea, Monilinia fructicola, Penicillium expansum and Aspergillus niger, whereas antibacterial activity was evaluated against Clavibacter michiganensis, Xanthomonas campestris, Pseudomonas savastanoi and P. syringae pv. phaseolicola. The chemical analysis has been carried out using GC-MS and the main components were identified as menthol (70.08%) and menthone (14.49%) followed by limonene (4.32%), menthyl acetate (3.76%) and β-caryophyllene (2.96%). The results show that the tested EO has promising antifungal activity against all tested fungi, whereas they demonstrated only a moderate antibacterial effect against some of the tested bacteria.
Advances in Genomics Approaches Shed Light on Crop Domestication
Authors: Zhao Yang , Feng Mengfan , Paudel Dev , Islam Tofazzal , Momotaz Aliya , Luo Ziliang , Zhao Zifan , Wei Ni , Li Sicheng , Xia Qing , Kuang Bowen , Yang Xiping , Wang Jianping
Published: August 2021

Crop domestication occurred ~10,000–12,000 years ago when humans shifted from a hunter–gatherer to an agrarian society. Crops were domesticated by selecting the traits in wild plant species that were suitable for human use. Research is crucial to elucidate the mechanisms and processes involved in modern crop improvement and breeding. Recent advances in genomics have revolutionized our understanding of crop domestication. In this review, we summarized cutting-edge crop domestication research by presenting its (1) methodologies, (2) current status, (3) applications, and (4) perspectives. Advanced genomics approaches have clarified crop domestication processes and mechanisms, and supported crop improvement.
Genome-Environment Association Analysis for Bio-Climatic Variables in Common Bean (Phaseolus vulgaris L.) from Brazil
Authors: Elias Júlio Cesar F. , Gonçalves-Vidigal Maria Celeste , Ariani Andrea , Valentini Giseli , Martiniano-Souza Maria da Conceição , Vaz Bisneta Mariana , Gepts Paul
Published: August 2021

Abiotic stress is a limiting factor for common bean (Phaseolus vulgaris L.) production globally. The study of the genotypic, phenotypic, and bio-climatic variables in a broad set of accessions may assist the identification of genomic regions involved in the climatic adaptation of the common bean. We conducted a genotyping-by-sequencing analysis using 28,823 SNPs on 110 georeferenced common bean accessions from Brazil to discover associations between SNPs and bio-climatic indexes. The population structure analysis clustered the accessions into two groups corresponding to the Andean and Mesoamerican gene pools. Of the 19 bioclimatic variables, 17 exhibited a significant association with SNPs on chromosomes Pv01, Pv02, Pv03, Pv04, Pv06, Pv09, Pv10, and Pv11 of common bean. Ten candidate genes were associated with specific bio-climatic variables related to temperature and precipitation. The candidate genes associated with this significant Pv09 region encode a Platz transcription factor family protein previously reported to be an essential regulator of drought stress. The SNP markers and candidate genes associated with the bio-climatic variables should be validated in segregating populations for water stress, which could further be used for marker-assisted selection. As a result, bean breeding programs may be able to provide advances in obtaining drought-tolerant cultivars.
Intra-Individual and Intraspecific Terpenoid Diversity in Erodium cicutarium
Authors: Eilers Elisabeth Johanna
Published: August 2021

The chemodiversity between and within individuals of several plant species is remarkable and shaped by the local habitat environment and the genetic background. The forb Erodium cicutarium (Geraniaceae) is globally distributed and partly invasive. This paper hypothesizes a high intra-specific and inter-individual chemical diversity in this species and investigates this by comparing the concentration and diversity of terpenoid compounds in different plant parts, i.e., leaves, blossoms and fruits. Plants were grown from seeds, originating from native range Bavaria (BY), Germany, and invaded range California (CA), USA, populations. In total, 20 different terpenoids were found, which occurred in distinct combinations and the patterns clustered into groups of distinct chemotypes for all plant parts. Several of the chemotypes were specific to plants of one region. The terpenoid compositions of different plant parts within individuals were highly correlated. Chemodiversity was higher in reproductive plant parts compared to the leaves, and higher in plants from BY compared to CA. This study highlights the intra-specific and inter-individual chemodiversity in E. cicutarium, linked to its geographical origin, which may facilitate its invasion success but also calls for further investigation of the role of chemodiversity in invasive plants on interactions with the environment.
Genetic Diversity for Agronomic Traits and Phytochemical Compounds in Coloured Naked Barley Lines
Authors: Iannucci Anna , Suriano Serafino , Codianni Pasquale
Published: August 2021

Interest of breeders is increasing toward the development of new barley cultivars with functional properties and adapted for food uses. A barley breeding program was initiated to develop germplasm with naked and coloured grains rich in bioactive compounds In the present study, a set of 16 F8 recombinant inbred lines (RILs) derived from the following four parental lines: 2005FG, K4-31, L94 and Priora, were evaluated in the experimental trials in Foggia (Italy) during the 2017–2019 growing seasons with the aims to assess the genetic variability for desired traits and identify superior genotypes. Lines were characterised for agronomic traits (earliness, plant height, seed yield, specific weight, 1000-seed weight) and biochemical compounds accumulation (phenolics, anthocyanins, flavonoids, carotenoids, β-glucans, proteins, antioxidant activity). A high heritability and selection response were observed for most of the biochemical compounds. The grain yield showed high significant positive genetic and phenotypic correlations (p < 0.05) with phenols and antioxidant activity. Cluster analysis grouped the genotypes into three groups. The barley RIL lines L1997, L3005, L3007 and L3009 were superior for more than four traits including seed yield and antioxidant compounds. These genotypes may serve as potential sources of nutraceuticals for healthy food and in breeding programs. In the present study, the new barley genotypes with naked and coloured grains have been selected without compromising their productivity.
Oxypeucedanin: Chemotaxonomy, Isolation, and Bioactivities
Authors: Mottaghipisheh Javad
Published: August 2021

The present review comprehensively gathered phytochemical, bioactivity, and pharmacokinetic reports on a linear furanocoumarin, namely oxypeucedanin. Oxypeucedanin (OP), which structurally contains an epoxide ring, has been majorly isolated from ethyl acetate-soluble partitions of several genera, particularly Angelica, Ferulago, and Prangos of the Apiaceae family; and Citrus, belonging to the Rutaceae family. The methanolic extract of Angelica dahurica roots has been analytically characterized as the richest natural OP source. This naturally occurring secondary metabolite has been described to possess potent antiproliferative, cytotoxic, anti-influenza, and antiallergic activities, as assessed in preclinical studies. In order to explore potential drug candidates, oxypeucedanin, its derivatives, and semi-synthetically optimized analogues can be considered for the complementary assessments of biological assays.
Projected Status of the Ghost Orchid (Dendrophylax lindenii) in Florida during the Next Decade Based on Temporal Dynamic Studies Spanning Six Years
Authors: Mújica Ernesto B. , Herdman Adam R. , Danaher Mark W. , González Elaine H. , Zettler Lawrence W.
Published: August 2021

The enigmatic ghost orchid, Dendrophylax lindenii (Lindley) Bentham ex Rolfe, is a showy leafless epiphyte restricted to low-lying forests in south Florida and western Cuba. Because of its appeal and reputation for being difficult to cultivate, D. lindenii remains vulnerable to poaching and environmental changes. About 2000 individuals are assumed to remain in Florida, most confined within water-filled cypress domes in the Fakahatchee Strand, but virtually no information exists on current population numbers throughout the region. This paper provides a preliminary summary of the ghost orchid’s projected status based on six continuous years of data collected within the Florida Panther National Wildlife Refuge (FPNWR) from 2015–2020. The orchids were clustered in seven different populations, each separated by ca. 5 km. Quantitative data were collected spanning three age classes (seedlings, juveniles, mature plants) for each population, and survival, flowering, and fruiting were noted. To estimate the temporal variability in the demographic rates, size-structured integral projection models (IPMs) were constructed for each annual transition (e.g., 2015–2016, 2016–2017). Results for all seven populations pooled suggest that D. lindenii numbers will decline by 20% during the next decade in the absence of external adverse factors. Seedling recruitment is not expected to keep pace with the projected decline. Only one population, which was also from the wettest location, continuously harbored spontaneous seedlings, suggesting that most populations within the FPNWR lack conditions suitable for reproduction.
Contribution to the Knowledge of Rocky Plant Communities of the Southwest Iberian Peninsula
Authors: Canas Ricardo Quinto , Cano-Ortiz Ana , Spampinato Giovanni , del Río Sara , Raposo Mauro , Fuentes José Carlos Piñar , Gomes Carlos Pinto
Published: August 2021

The rocky habitats of southern Portugal are ecosystems with extreme xericity conditions, associated with special abiotic strains. In these unstable ecological conditions, a considerable diversity of plant communities occurs. The objective of this study, carried out in the Algarve and Monchique, and the Mariánica Range biogeographical sectors, is to compare chasmo-chomophytic communities of the southwestern Iberian Peninsula, using a phytosociological approach (Braun–Blanquet methodology) and numerical analysis (hierarchical cluster analysis). From these results, two new communities were identified, Sanguisorbo rupicolae-Dianthetum crassipedis and Antirrhinetum onubensis, as a result of floristic and biogeographical differences from other associations already described within the alliances Rumici indurati-Dianthion lusitani and Calendulo lusitanicae-Antirrhinion linkiani, both included in the Phagnalo saxatilis-Rumicetea indurate class.
Redesign of the Geometry of Parts Produced from PBT Composite to Improve Their Operational Behavior
Authors: Dobrotă Dan , Lazăr Sergiu Viorel
Published: August 2021

Parts produced from PBT-GF30 (70% polybutylene terephthalate +30% fiberglass) are very often used in car construction, due to the properties of this material. The current trend is to make parts with a shape designed to be as complex as possible, to take over many functions in operation. During the research, a part that is a component of the structure of car safety systems, and that must be completely reliable in operation, was analyzed. This piece has a complex shape that involves the intersection of several walls. Thus, the research aimed at establishing the optimal radius of connection between the walls (R), the ratio between the thickness of the intersecting walls (K) and the angle of inclination of the walls (α). The composite central design method was used to design the experiments. Both new parts and parts subject to an artificial aging process were tested. All parts were subjected to shear stress, to determine the load (L) and displacement (D) at which they break. In order to observe other changes in the properties of the parts, in addition to the mechanical ones, an analysis of the color of the new and aged parts was performed, as well as a topography of the surface layer in the breaking area. The design of the parts involved changes to the parameters of the injection process. In these conditions, a PBT-GF30 viscosity analysis was performed for new and artificially aged parts.
A Cyclic BMP-2 Peptide Upregulates BMP-2 Protein-Induced Cell Signaling in Myogenic Cells
Authors: Gudivada Vijaya Narasimha , Huang Chen-Ji , Luo Yueh-Hsia , Dong Guo-Chung
Published: August 2021

In the current study, we designed four cyclic peptide analogues by incorporating two cysteine residues in a BMP-2 linear knuckle epitope in such a way that the active region of the peptide could be either inside or outside the cyclic ring. Bone morphogenetic protein receptor BMPRII was immobilized on the chip surface, and the interaction of the linear and cyclic peptide analogues was studied using surface plasmon resonance (SPR). From the affinity data, the peptides with an active region inside the cyclic ring had a higher binding affinity in comparison to the other peptides. To confirm that our affinity data are in line in vitro, we studied the expression levels of RUNX2 (runt-related transcription factor) and conducted an osteogenic marker alkaline phosphatase (ALP) assay and staining. Based on the affinity data and the in vitro experiments, peptide P-05 could be a suitable candidate for osteogenesis, with higher binding affinity and increased RUNX2 and ALP expression in comparison to the linear peptides.
Preparation of Poly(ethylene glycol)@Polyurea Microcapsules Using Oil/Oil Emulsions and Their Application as Microreactors
Authors: Zarour Ahmad , Omar Suheir , Abu-Reziq Raed
Published: August 2021

The development process of catalytic core/shell microreactors, possessing a poly(ethylene glycol) (PEG) core and a polyurea (PU) shell, by implementing an emulsion-templated non-aqueous encapsulation method, is presented. The microreactors’ fabrication process begins with an emulsification process utilizing an oil-in-oil (o/o) emulsion of PEG-in-heptane, stabilized by a polymeric surfactant. Next, a reaction between a poly(ethylene imine) (PEI) and a toluene-2,4-diisocyanate (TDI) takes place at the boundary of the emulsion droplets, resulting in the creation of a PU shell through an interfacial polymerization (IFP) process. The microreactors were loaded with palladium nanoparticles (NPs) and were utilized for the hydrogenation of alkenes and alkynes. Importantly, it was found that PEG has a positive effect on the catalytic performance of the developed microreactors. Interestingly, besides being an efficient green reaction medium, PEG plays two crucial roles: first, it reduces the palladium ions to palladium NPs; thus, it avoids the unnecessary use of additional reducing agents. Second, it stabilizes the palladium NPs and prevents their aggregation, allowing the formation of highly reactive palladium NPs. Strikingly, in one sense, the suggested system affords highly reactive semi-homogeneous catalysis, whereas in another sense, it enables the facile, rapid, and inexpensive recovery of the catalytic microreactor by simple centrifugation. The durable microreactors exhibit excellent activity and were recycled nine times without any loss in their reactivity.
Long-Term Cultivation of a Native Arthrospira platensis (Spirulina) Strain in Pozo Izquierdo (Gran Canaria, Spain): Technical Evidence for a Viable Production of Food-Grade Biomass
Authors: Guidi Flavio , Gojkovic Zivan , Venuleo Marianna , Assunçao Patrícia Alexandra Clemente Janeiro , Portillo Eduardo
Published: August 2021

Microalgae cultivation is a promising alternative to traditional agriculture in arid—semi-arid areas. The aim of this study is to assess the viability of long-term cultivation of native Arthrospira platensis in Gran Canaria. Maximum culture productivity (0.08 g/L/day) and optimal concentration range (0.6–0.9 g/L) were firstly determined in 8000 L raceway under a greenhouse. Afterwards, a stable productivity of 0.06 g/L/day (6.0 g/m2/day) was obtained by reusing the culture medium during 26 days of cultivation, with consistent biomass biochemical composition. Outdoor temperature and daily solar irradiation ranged between 17.9–30.7 °C and 79.2–274.8 W/m2, while culture pH and salinity were in the range 9.42–10.77 and 11.2–14.9 g/L, respectively. Protein (>60%), potassium (1.8 g/100 g) and C-phycocyanin (7.2%) content is in the high-range of commercial Spirulina, which makes BEA 1257B promising for food and extraction of natural pigments/antioxidants. The dried biomass complies with international standards for human consumption, because of low heavy metal content and no pathogens presence. Product quality can be improved by reducing ash (≃12%) and sodium (1.5%) content through biomass washing optimization and/or further dewatering step. Other microorganisms can be prevented by high alkaline conditions and mild chemical treatments. These results pave the way for a sustainable microalgae-based blue bioeconomy in the Canary Islands.
Application of Anammox-Based Processes in Urban WWTPs: Are We on the Right Track?
Authors: Pedrouso Alba , Vázquez-Padín José Ramón , Crutchik Dafne , Campos José Luis
Published: August 2021

The application of partial nitritation and anammox processes (PN/A) to remove nitrogen can improve the energy efficiency of wastewater treatment plants (WWTPs) as well as diminish their operational costs. However, there are still several limitations that are preventing the widespread application of PN/A processes in urban WWTPs such as: (a) the loss of performance stability of the PN/A units operated at the sludge line, when the sludge is thermally pretreated to increase biogas production; (b) the proliferation of nitrite-oxidizing bacteria (NOB) in the mainstream; and (c) the maintenance of a suitable effluent quality in the mainstream. In this work, different operational strategies to overcome these limitations were modelled and analyzed. In WWTPs whose sludge is thermically hydrolyzed, the implementation of an anerobic treatment before the PN/A unit is the best alternative, from an economic point of view, to maintain the stable performance of this unit. In order to apply the PN/A process in the mainstream, the growth of ammonia-oxidizing bacteria (AOB) should be promoted in the sludge line by supplying extra sludge to the anaerobic digesters. The AOB generated would be applied to the water line to partially oxidize ammonia, and the anammox process would then be carried out. Excess nitrate generated by anammox bacteria and/or NOB can be removed by recycling a fraction of the WWTP effluent to the biological reactor to promote its denitrification.
Study on the Flow Characteristics of Desulfurization Ash Fine Particles in a Circulating Fluidized Bed
Authors: Yang Xiao , Wang Chengxiu , Lan Xingying , Gao Jinsen
Published: August 2021

In view of the current status of catalytic cracking flue gas treatment, it is necessary to study the flow environment of desulfurization ash particles, which are a type of Geldart C particle, in a circulating fluidized bed (CFB) for semi-dry flue gas desulphurization using CFB technology. This study investigated the flow characteristics of desulphurization ash particles in a riser with an inner diameter of 70 mm and a height of 12.6 m, at a gas velocity of 4–7 m/s and a solids circulation rate of 15–45 kg/m2·s. The solids holdup in the axial distribution is relatively high near the bottom of the riser, and gradually decreases as the riser height increases, with a stable value from the middle to the top of the riser. In the radial distribution, the solids holdup of desulfurization ash particles is low in the center and high in the wall region. Within the above operating conditions, the solids holdup ranges from 0.008 to 0.025. The particle-based Archimedes number has a linear relationship with the solids holdup at all operating conditions.
The Topic of the Ideal Dairy Farm Can Inspire How to Assess Knowledge about Dairy Production Processes: A Case Study with Students and Their Contributions
Authors: Gaworski Marek , de Cacheleu Chloé , Inghels Clémentine , Leurs Lucie , Mazarguil Camille , Ringot Bertille , Tzu-Chen Chiu
Published: August 2021

The dairy farm and on-farm production processes are the subject of numerous evaluations. These are not only evaluations of the economic efficiency of milk production. Opinions expressed by various social groups are also an important contribution to improving the approach to milk production on the farm. As a result of such opinions, a vision of an ideal dairy farm may be formed. The aim of the study was to develop the thematic area of an ideal dairy farm in the opinion of two groups of students who were learning remotely (in the distance learning system) due to the Covid-19 pandemic. The first group consisted of six Erasmus+ students. The second group consisted of 70 full-time Polish students. As part of their homework, the students answered three questions about the ideal dairy farm. Students had 4 weeks to do their homework. Erasmus+ students’ homework was used to propose a ranking method for assessing the answers to three questions by the students themselves. Homework of Polish students was used to analyze the frequency of using certain keywords. Polish students, in their homework on the ideal dairy farm, mainly used the basic concepts related to dairy production. Unfortunately, a very small number of students used terms that represent a responsible approach to dairy production, including ethical aspects, freedom, sustainability, animal pain, antibiotics, and organic milk production. In conclusion, it was indicated that the curriculum should be structured in such a way as to raise students’ awareness of dairy production and its current challenges.
Retelling Mecca: Shifting Narratives of Sacred Spaces in Volga-Ural Muslim Hajj Accounts, 1699–1945
Authors: Ross Danielle
Published: August 2021

This article examines how Volga-Ural Muslims narrated their encounters with the sacred spaces visited during the hajj. It examines nine accounts hajj composed from the 1690s to the 1940s, to consider how changes in international politics, Russia’s domestic politics, and the culture of Islamic learning within the VolgaUral Muslim community led to writers to revise narratives of why the sacred spaces of Mecca were sacred, how best to experience the power of these sacred spaces, and how these sacred spaces fit into the local culture of Volga-Ural Islam under Russian and Soviet rule.
Reading Karl Barth on Truth and Falsehood in the Post-Truth Age
Authors: Thompson Geoff
Published: August 2021

This article offers a close reading of two sections of Karl Barth’s Church Dogmatics, i.e., §70.1 “The True Witness” and §70.2 “The Falsehood of Man” against the background of the post-truth environment. A brief discussion of the post-truth phenomenon highlights how some strands of the resistance to it trade on a binary of objective and subjective approaches to truth and epistemology, insisting on the triumph of the former over the latter as the way of overcoming the problems of knowledge and truth in a post-truth culture. The reading of the two selected texts from the Dogmatics indicate that Barth’s discussion of truth and falsehood cuts across that binary. Whilst much of what Barth says in these texts is said in earlier parts of the Dogmatics, it is sharpened in this context by Barth’s discussion of the “pious lie,” the distortion of the truth within the Christian community, as the fundamental form of falsehood. Alertness to this sin challenges the church to adopt a posture of self-criticism to its own knowledge of the truth. This can be its own form of witness in the post-truth age.
CO2 Flux over the Contiguous United States in 2016 Inverted by WRF-Chem/DART from OCO-2 XCO2 Retrievals
Authors: Zhang Qinwei , Li Mingqi , Wang Maohua , Mizzi Arthur , Huang Yongjian , Wei Chong , Jin Jiuping , Gu Qianrong
Published: August 2021

High spatial resolution carbon dioxide (CO2) flux inversion systems are needed to support the global stocktake required by the Paris Agreement and to complement the bottom-up emission inventories. Based on the work of Zhang, a regional CO2 flux inversion system capable of assimilating the column-averaged dry air mole fractions of CO2 (XCO2) retrieved from Orbiting Carbon Observatory-2 (OCO-2) observations had been developed. To evaluate the system, under the constraints of the initial state and boundary conditions extracted from the CarbonTracker 2017 product (CT2017), the annual CO2 flux over the contiguous United States in 2016 was inverted (1.08 Pg C yr−1) and compared with the corresponding posterior CO2 fluxes extracted from OCO-2 model intercomparison project (OCO-2 MIP) (mean: 0.76 Pg C yr−1, standard deviation: 0.29 Pg C yr−1, 9 models in total) and CT2017 (1.19 Pg C yr−1). The uncertainty of the inverted CO2 flux was reduced by 14.71% compared to the prior flux. The annual mean XCO2 estimated by the inversion system was 403.67 ppm, which was 0.11 ppm smaller than the result (403.78 ppm) simulated by a parallel experiment without assimilating the OCO-2 retrievals and closer to the result of CT2017 (403.29 ppm). Independent CO2 flux and concentration measurements from towers, aircraft, and Total Carbon Column Observing Network (TCCON) were used to evaluate the results. Mean bias error (MBE) between the inverted CO2 flux and flux measurements was 0.73 g C m−2 d−1, was reduced by 22.34% and 28.43% compared to those of the prior flux and CT2017, respectively. MBEs between the CO2 concentrations estimated by the inversion system and concentration measurements from TCCON, towers, and aircraft were reduced by 52.78%, 96.45%, and 75%, respectively, compared to those of the parallel experiment. The experiment proved that CO2 emission hotspots indicated by the inverted annual CO2 flux with a relatively high spatial resolution of 50 km consisted well with the locations of most major metropolitan/urban areas in the contiguous United States, which demonstrated the potential of combing satellite observations with high spatial resolution CO2 flux inversion system in supporting the global stocktake.
A Novel Modeling Strategy of Weighted Mean Temperature in China Using RNN and LSTM
Authors: Gao Wenliang , Gao Jingxiang , Yang Liu , Wang Mingjun , Yao Wenhao
Published: August 2021

In the meteorology of Global Navigation Satellite System, the weighted mean temperature (Tm) is a key parameter in the process of converting the zenith wetness delay into precipitable water vapor, and it plays an important role in water vapor monitoring. In this research, two deep learning algorithms, namely, recurrent neural network (RNN) and long short-term memory neural network (LSTM), were used to build a high-precision weighted mean temperature model for China using their excellent time series memory capability. The model needs site location information and measured surface temperature to predict the weighted mean temperature. We used data from 118 stations in and around China provided by the Integrated Global Radiosonde Archive from 2010 to 2015 to train the model and data from 2016 for model testing. The root mean square error (RMSE) of the RNN_Tm and LSTM_Tm models were 3.01 K and 2.89 K, respectively. Compared with the values calculated by the empirical GPT3 model, the accuracy was improved by 31.1% (RNN_Tm) and 33.9% (LSTM_Tm). In addition, we selected another 10 evenly distributed stations in China and used the constructed model to test the prediction capability of the weighted mean temperature from 2010 to 2016. The RMSE values were 2.95 K and 2.86 K, which proved that the model also exhibits high generalization in non-modeling sites in China. In general, the RNN_Tm and LSTM_Tm models have a good performance in weighted mean temperature prediction.
RSPD: A Novel Remote Sensing Index of Plant Biodiversity Combining Spectral Variation Hypothesis and Productivity Hypothesis
Authors: Sun Hao , Hu Jiaqi , Wang Jiaxiang , Zhou Jingheng , Lv Ling , Nie Jingyan
Published: August 2021

Plant diversity (PD) plays an important role in maintaining the healthy function of an ecosystem through affecting the productivity, stability, and nutrient utilization of a terrestrial ecosystem. Remote sensing is a vital way to monitor the status and changes of PD. Most of the existing methods rely on a field botany survey to construct a statistical relationship between PD and remote sensing observations. However, a field botany survey is too costly to be applied widely. In this study, we constructed a new remote sensing index of PD (RSPD), combining the spectral variation hypothesis and productivity hypothesis. Concretely, the RSPD integrated the multi-band spectral reflectance and several spectral greenness, moisture, and red-edge vegetation indices with the principles of Shannon information entropy and Euclidean distance. The RSPD was evaluated by comparing the classical coefficient of variation (CV) method and the Shannon and Simpson diversity indices based on vegetation classification results. Two cases were selected, where Case I was in Beijing and Case II was located in part of Huai’an, China. Sentinel-2 data in three years of 2016, 2018, and 2020 and higher-resolution Pléiades-1 data in 2018 were also utilized. The results demonstrate that: (1) the RSPD is basically consistent with the CV in spatiotemporal variation; (2) the RSPD outperforms the CV as compared with Shannon and Simpson diversity indices that are based on vegetation classification results with Sentinel-2 and Pléiades-1 data; (3) the RSPD outperforms the CV as compared with visual interpretations with Google Earth image. The suggested index can reflect the richness and evenness of plant species, which is inherent in its calculation formula. Moreover, it has a great potential for large-scale regional and long-term series monitoring.