bluuebunny/crossref_metadata_2025 huggingface dataset:
The Prognostic Value of Pre-treatment Hemoglobin (Hb) in Patients With Advanced or Metastatic Gastric Cancer Treated With Immunotherapy
Authors: Gou Miaomiao , Zhang Yong , Liu Tiee , Qu Tongtong , Si Haiyan , Wang Zhikuan , Yan Huan , Qian Niansong , Dai Guanghai
Published: June 2021

Background

Biomarkers such as prevailing PD-L1 expression and TMB have been proposed as a way of predicting the outcome of immunotherapy in patients with advanced gastric cancer (AGC) and metastatic gastric cancer (MGC). Our study aims to investigate whether there is a link between pretreatment hemoglobin (Hb) levels and survival to immunotherapy in patients with AGC and MGC.

Methods

We retrospectively reviewed patients with AGC or MGC treated at the oncology department of the Chinese PLA general hospital receiving PD-1 inhibitor. The Propensity Score Matching (PSM) (1:1) was performed to balance potential baseline confounding factors. Progression-free survival (PFS) and overall survival (OS) was analyzed among different Hb level (normal Hb group and decreased Hb group). Objective response rate (ORR), disease control rate (DCR) were also analyzed. Univariate analysis and multivariate analysis were performed further to validate the prognostic value of Hb level.

Results

We included 137 patients with AGC and MGC who received PD-1 inhibitors (including Pembrolizumab, Nivolumab, Sintilimab, Toripalimab) in this study. After PSM matching, there were no significant differences between the two groups for baseline characteristics. Within the matched cohort, the median PFS was 7.8 months in the normal Hb level group and 4.3 months in the decreased Hb group (HR 95% CI 0.5(0.31, 0.81), P=0.004). The OS was 14.4 months with normal Hb level as compared with 8.2 months with decreased Hb level(HR 95% CI 0.59(0.37, 0.94), P=0.024). The ORR was 40.7% and DCR was 83.0% in the normal Hb group, while the ORR was 25.5% and DCR was 85.1% in the decreased Hb group. No significant differences were found in the ORR and DCR between the two groups (P=0.127, P=0.779). Univariate analysis and multivariate analysis showed that Hb level was only independent predictor for PFS and baseline Hb level was significant prognostic factor influencing the OS. Only when patients had normal Hb level, anti-pd-1 monotherapy or combined with chemotherapy was superior to anti-pd-1 plus anti-angiogenic therapy with respect to PFS (10.3 m vs 2.8 m, HR 95% CI 0.37(0.15, 0.95), P=0.031) and OS(15 m vs 5.7 m, HR 95% CI 0.21 (0.08, 0.58), P=0.001).

Conclusions

Our study have demonstrated that pretreatment Hb level was an independent prognostic biomarker in term of PFS and OS with immunotherapy for AGC and MGC patients. Correction of anemia for GC patients as immunotherapy would be a strategy to improve the survival. More data was warranted to further influence this finding.
Outstanding Response in a Patient With ROS1-Rearranged Inflammatory Myofibroblastic Tumor of Soft Tissues Treated With Crizotinib: Case Report
Authors: Comandini Danila , Catalano Fabio , Grassi Massimiliano , Pesola Guido , Bertulli Rossella , Guadagno Antonio , Spina Bruno , Mascherini Matteo , De Cian Franco , Pistoia Federico , Rebuzzi Sara Elena
Published: June 2021

Inflammatory myofibroblastic tumor (IMT) is a very rare subtype of sarcoma, which frequently harbor chromosomal rearrangements, including anaplastic lymphoma kinase (ALK) rearrangements (almost 50% of the IMTs) and other kinase fusions such as ROS1. ROS1 fusions are present in about 10% of IMT, almost half of the ALK-negative IMT patients. Apart from radical surgery for resectable tumors, there is no standard-of-care therapy for advanced IMTs. Nonetheless, the use of tyrosine kinase inhibitors has shown promising efficacy in IMT patients with targetable genomic alterations. We report the case of a 24-year-old patient with chemotherapy-refractory metastatic IMT harboring ROS1 kinase fusion, who experienced a significant clinical and pathological response to crizotinib. This clinical case highlights the need to assess all patients with unresectable IMTs for chromosomal abnormalities and gene mutations and address them to targeted agents as well as clinical trials.
Prospective Assessment of Early Proton Therapy-Induced Optic Neuropathy in Patients With Intracranial, Orbital or Sinonasal Tumors: Impact of A Standardized Ophthalmological Follow Up
Authors: Lecornu Marie , Lesueur Paul , Salleron Julia , Balosso Jacques , Stefan Dinu , Kao William , Plouhinec Tiphaine , Vela Anthony , Dutheil Pauline , Bouter Jordan , Marty Pierre-Alban , Thariat Juliette , Quintyn Jean-Claude
Published: June 2021

Purpose

Proton therapy (PT) can be a good option to achieve tumor control while reducing the probability of radiation induced toxicities compared to X-ray-based radiotherapy. However, there are still uncertainties about the effects of PT on the organs in direct contact with the irradiated volume. The aim of this prospective series was to report 6-month follow-up of clinical and functional optic neuropathy rates of patients treated by proton therapy using a standardized comprehensive optic examination.

Methods and Materials

Standardized ophthalmological examinations were performed to analyze subclinical anomalies in a systematic way before treatment and 6 months after the end of proton therapy with: Automatic visual field, Visual evoked potential (VEP) and optic coherence of tomography (OCT).

Results

From October 2018 to July 2020 we analyzed 81 eyes. No significant differences were found in the analysis of the clinical examination of visual functions by the radiation oncologist. However, considering VEP, the impairment was statistically significant for both fibers explored at 30’angle (p:0.007) and 60’angle (p <0.001). In patients with toxicity, the distance of the target volume from the optical pathways was more important with a p-value for 30’VEP at 0.035 and for 60’VEP at 0.039.

Conclusions

These results confirm uncertainties concerning relative biological effectiveness of proton therapy, linear energy transfer appears to be more inhomogeneous especially in areas close to the target volumes. The follow-up of patients after proton therapy is not an easy process to set up but it is necessary to improve our knowledges about the biological effects of proton therapy in real life. Our study which will continue during the coming years, suggests that follow-up with in-depth examinations such as VEP as a biomarker could improve the detection of early abnormalities.
RNA N6-Methyladenosine in Cancer Metastasis: Roles, Mechanisms, and Applications
Authors: Dang Qin , Shao Bo , Zhou Quanbo , Chen Chen , Guo Yaxin , Wang Guixian , Liu Jinbo , Kan Quancheng , Yuan Weitang , Sun Zhenqiang
Published: June 2021

Cancer metastasis is a symptom of adverse prognosis, a prime origin of therapy failure, and a lethal challenge for cancer patients. N6-methyladenosine (m6A), the most prevailing modification in messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs) of higher eukaryotes, has attracted increasing attention. Growing studies have verified the pivotal roles of m6A methylation in controlling mRNAs and ncRNAs in diverse physiological processes. Remarkably, recent findings have showed that aberrant methylation of m6A-related RNAs could influence cancer metastasis. In this review, we illuminate how m6A modifiers act on mRNAs and ncRNAs and modulate metastasis in several cancers, and put forward the clinical application prospects of m6A methylation.
Osteoking Decelerates Cartilage Degeneration in DMM-Induced Osteoarthritic Mice Model Through TGF-β/smad-dependent Manner
Authors: Ling Houfu , Zeng Qinghe , Ge Qinwen , Chen Jiali , Yuan Wenhua , Xu Rui , Shi Zhenyu , Xia Hanting , Hu Songfeng , Jin Hongting , Wang Pinger , Tong Peijian
Published: June 2021

Osteoarthritis (OA) is a common disease characterized by cartilage degeneration. In recent years much attention has been paid to Traditional Chinese Medicine (TCM) since its treatments have shown efficacy for ameliorating cartilage degradation with mild side effects. Osteoking is a TCM prescription that has long been used in OA treatment. However, the exact mechanism of Osteoking are not fully elucidated. In the current study, destabilization of the medial meniscus (DMM)-induced OA mice was introduced as a wild type animal model. After 8 weeks of administration of Osteoking, histomorphometry, OARSI scoring, gait analysis, micro-CT, and immunohistochemical staining for Col2, MMP-13, TGFβRII and pSmad-2 were conducted to evaluate the chondroprotective effects of Osteoking in vivo. Further in vitro experiments were then performed to detect the effect of Osteoking on chondrocytes. TGFβRIICol2ER transgenic mice were constructed and introduced in the current study to validate whether Osteoking exerts its anti-OA effects via the TGF-β signaling pathway. Results demonstrated that in wild type DMM mice, Osteoking ameliorated OA-phenotype including cartilage degradation, subchondral bone sclerosis, and gait abnormality. Col2, TGFβRII, and pSmad-2 expressions were also found to be up-regulated after Osteoking treatment, while MMP-13 was down-regulated. In vitro, the mRNA expression of MMP-13 and ADAMTS5 decreased and the mRNA expression of Aggrecan, COL2, and TGFβRII were up-regulated after the treatment of Osteoking in IL-1β treated chondrocytes. The additional treatment of SB505124 counteracted the positive impact of Osteoking on primary chondrocytes. In TGFβRIICol2ER mice, spontaneous OA-liked phenotype was observed and treatment of Osteoking failed to reverse the OA spontaneous progression. In conclusion, Osteoking ameliorates OA progression by decelerating cartilage degradation and alleviating subchondral bone sclerosis partly via the TGF-β signaling pathway.
Knowledge of Chosen Family History and Depressive Symptoms in Sexual Minority Women
Authors: Gardella Jamie M. , Parnes McKenna F. , Hirst William , Brown Adam D.
Published: June 2021

Recent work on intergenerational memory has revealed a positive association between family of origin knowledge and wellbeing in adolescents. However, little is known about the generalizability of these data, as significantly less attention has focused on autobiographical memory sharing and wellbeing in historically marginalized communities. Given the high incidence of familial rejection and abandonment within the LGBTQIA + community, close relationships with individuals outside of one’s family of origin, chosen families, often serve as an important source of social support. This study sought to examine the relationship between knowledge of a close non-family member and wellbeing among emerging adult sexual minority women (SMW) according to their gender presentation. A community sample from New York City comprised of heterosexual women (n = 50), masculine-presenting SMW (n = 50), and feminine presenting SMW (n = 50) completed measures associated with their knowledge of their family of origin, knowledge of a close non-family member, as well as self-reported measures of depression, emotion regulation, and socio-demographic questions. Family of origin knowledge was associated with lower levels of depression only among heterosexual women. However, heterosexual and SMW who knew more about their close non-family member reported lower levels of depression. Additionally, emotion regulation (cognitive reappraisals) mediated the relationship between knowing more about one’s chosen family and lower depressive symptom severity among heterosexual women, but this relationship was only significant for SMW who were at least moderately open about their sexuality. These findings extend the literature on the benefits of memory sharing to historically marginalized communities by showing that memory sources outside of one’s family of origin may be particularly important. Additionally, these data begin to shed light on potential mediating factors, such as emotion regulation and openness about one’s sexual identity, that underlie the links between memory sharing and metrics of wellbeing. Taken together, in contexts in which there may not be opportunities to learn about family history from one’s family of origin, it appears that access to stories from someone close outside of one’s family is also associated with lower levels of depression.
Family Sibling Effect and Executives’ Corporate Social Behavior
Authors: Zheng Minna , Ren Guangqian , Zhuang Lingling
Published: June 2021

Corporate social responsibility is an important business strategy for enterprises. Scholars have conducted much beneficial research on the relationship of executives’ recognitive traits and firms’ CSR behavior, but rarely focus on the impact of executives’ early recognitive traits derived from family sibling interaction. This paper takes Chinese A-shared private listed companies from 2014 to 2017 as the research samples to investigate the effect of the number of executives’ siblings on the early family sibling and corporate social responsibility behavior. We further study the moderating effect of birth order and gender composition in siblings on this relationship. The results show that there is an inversed U-shaped relationship between the number of executives’ siblings and corporate social responsibility behavior. Further research shows that the relationship between the number of executives’ siblings and CSR behavior is strengthened when an executive is first-born or has female sibling(s).
Prognostic Role of Neutrophil to Lymphocyte Ratio in COVID-19 Patients: Still Valid in Patients That Had Started Therapy?
Authors: Gelzo Monica , Cacciapuoti Sara , Pinchera Biagio , De Rosa Annunziata , Cernera Gustavo , Scialò Filippo , Mormile Mauro , Fabbrocini Gabriella , Parrella Roberto , Gentile Ivan , Castaldo Giuseppe
Published: June 2021

COVID-19 may appear with a widely heterogeneous clinical expression. Thus, predictive markers of the outcome/progression are of paramount relevance. The neutrophil/lymphocyte ratio (NLR) has been suggested as a good predictive marker of disease severity and mortality. Accordingly, we found that NLR significantly increased in parallel with the WHO severity stage in COVID-19 patients during the Ist wave (March-May 2020; n = 49), due to the significant reduction of lymphocyte and the significant increase of neutrophil in severe COVID-19 patients. While, we did not observe significant differences of NLR between the WHO severity stage among COVID-19 patients of the IInd wave (September 2020-April 2021; n = 242). In these patients, the number of lymphocytes and neutrophils did not change significantly between patients of different severity subgroups. This difference likely depends on the steroids therapy that the patients of the IInd wave performed before hospitalization while most patients of the Ist wave were hospitalized soon after diagnosis. This is also confirmed by serum interleukin (IL)-6 and myeloperoxidase (MPO) that gradually increased with the disease stage in patients of the Ist wave, while such biomarkers (whose production is inhibited by steroids) did not show differences among patients of the IInd wave in different stages. Thus, the NLR could be tested at diagnosis in naïve patients before starting therapies.
What We “Cannot Not Know in America”: 400 Years of Inequality and Seven Sins
Authors: Sember Robert , Fullilove Mindy Thompson , Fullilove Robert E.
Published: June 2021

The 400 Years of Inequality Project was created to call organizations to observe the 400th anniversary of the first Africans landing in Jamestown in 1619. The project focused on the broad ramifications of inequality. Used as a justification of chattel slavery, structures of inequality continue to condition the lives of many groups in the US. Over 110 organizations joined this observance and held 150 events. The highlight of the year was the homily given by Reverend William Barber II, co-chair of the Poor People's Campaign, who described the “seven sins” that link the concept of inequality to every aspect of national life, from politics to militia. These “seven sins” help us to analyze and address crises, such as the COVID pandemic.
Retrospective Study on the Influencing Factors and Prediction of Hospitalization Expenses for Chronic Renal Failure in China Based on Random Forest and LASSO Regression
Authors: Dai Pingping , Chang Weifu , Xin Zirui , Cheng Haiwei , Ouyang Wei , Luo Aijing
Published: June 2021

Aim: With the improvement in people's living standards, the incidence of chronic renal failure (CRF) is increasing annually. The increase in the number of patients with CRF has significantly increased pressure on China's medical budget. Predicting hospitalization expenses for CRF can provide guidance for effective allocation and control of medical costs. The purpose of this study was to use the random forest (RF) method and least absolute shrinkage and selection operator (LASSO) regression to predict personal hospitalization expenses of hospitalized patients with CRF and to evaluate related influencing factors.

Methods: The data set was collected from the first page of data of the medical records of three tertiary first-class hospitals for the whole year of 2016. Factors influencing hospitalization expenses for CRF were analyzed. Random forest and least absolute shrinkage and selection operator regression models were used to establish a prediction model for the hospitalization expenses of patients with CRF, and comparisons and evaluations were carried out.

Results: For CRF inpatients, statistically significant differences in hospitalization expenses were found for major procedures, medical payment method, hospitalization frequency, length of stay, number of other diagnoses, and number of procedures. The R2 of LASSO regression model and RF regression model are 0.6992 and 0.7946, respectively. The mean absolute error (MAE) and root mean square error (RMSE) of the LASSO regression model were 0.0268 and 0.043, respectively, and the MAE and RMSE of the RF prediction model were 0.0171 and 0.0355, respectively. In the RF model, and the weight of length of stay was the highest (0.730).

Conclusions: The hospitalization expenses of patients with CRF are most affected by length of stay. The RF prediction model is superior to the LASSO regression model and can be used to predict the hospitalization expenses of patients with CRF. Health administration departments may consider formulating accurate individualized hospitalization expense reimbursement mechanisms accordingly.
Evaluation of Antigenic Comparisons Among BVDV Isolates as it Relates to Humoral and Cell Mediated Responses
Authors: Falkenberg Shollie M. , Dassanayake Rohana P. , Terhaar Brett , Ridpath Julia F. , Neill John D. , Roth James A.
Published: June 2021

Antigenic differences between bovine viral diarrhea virus (BVDV) vaccine strains and field isolates can lead to reduced vaccine efficacy. Historically, antigenic differences among BVDV strains were evaluated using techniques based on polyclonal and monoclonal antibody activity. The most common method for antigenic comparison among BVDV isolates is determination of virus neutralization titer (VNT). BVDV antigenic comparisons using VNT only account for the humoral component of the adaptive immune response, and not cell mediated immunity (CMI) giving an incomplete picture of protective responses. Currently, little data is available regarding potential antigenic differences between BVDV vaccine strains and field isolates as measured by CMI responses. The goal of the current paper is to evaluate two groups of cattle that differed in the frequency they were vaccinated, to determine if similar trends in CMI responses exist within each respective group when stimulated with antigenically different BVDV strains. Data from the current study demonstrated variability in the CMI response is associated with the viral strain used for stimulation. Variability in IFN-γ mRNA expression was most pronounced in the CD4+population, this was observed between the viruses within each respective BVDV subgenotype in the Group 1 calves. The increase in frequency of CD25+cells and IFN-γ mRNA expression in the CD8+and CD335+populations were not as variable between BVDV strains used for stimulation in the Group 1 calves. Additionally, an inverse relationship between VNT and IFN-γ mRNA expression was observed, as the lowest VNT and highest IFN-γ mRNA expression was observed and vice versa, the highest VNT and lowest IFN-γ mRNA expression was observed. A similar trend regardless of vaccination status was observed between the two groups of calves, as the BVDV-1b strain had lower IFN-γ mRNA expression. Collectively, data from the current study and previous data support, conferring protection against BVDV as a method for control of BVDV in cattle populations is still a complex issue and requires a multifactorial approach to understand factors associated with vaccine efficacy or conversely vaccine failure. Although, there does appear to be an antigenic component associated with CMI responses as well as with humoral responses as determined by VNT.
Assessment of Platelet and Plasma Serotonin in Canine Pulmonary Hypertension Secondary to Degenerative Mitral Valve Disease
Authors: Tangmahakul Nattawan , Makoom Pussadee , Surachetpong Sirilak Disatian
Published: June 2021

Background: Pulmonary hypertension (PH) is a common complication of degenerative mitral valve disease (DMVD), the most common cardiovascular disease in dogs. Serotonin has been suspected to play a role in the pathogenesis of PH, so this study aimed to investigate the differences in platelet and plasma serotonin between normal, DMVD and DMVD with PH (DMVD+PH) dogs.

Materials and Methods: Sixty-two small-breed dogs were enrolled to the study and divided into the normal (n = 22), DMVD (n = 20), and DMVD+PH (n = 20) groups. The platelet and plasma serotonin concentrations were measured by the competitive ELISA.

Results: The Kruskal–Wallis revealed the difference among the four groups of normal (179.73 [102.37–352.24] ng/109 platelets), DMVD (325.99 [96.84–407.66] ng/109 platelets), DMVD with intermediate probability of PH (291.11 [106.69–400.84] ng/109 platelets) and DMVD with high probability of PH (35.82 [2.69–126.35] ng/109 platelets) (p = 0.014). The Dunn's post-hoc test showed a decrease in the platelet serotonin concentration of the DMVD dogs with high probability of PH compared to the DMVD group (p = 0.008). The plasma serotonin concentration was not different between normal, DMVD, and DMVD+PH dogs.

Conclusion: In conclusion, a decrease in platelet serotonin concentration, which is associated with a degree of PH probability was found in DMVD dogs with PH. Further studies investigating roles of platelet serotonin in PH secondary to DMVD should be performed.
Preliminary Determination of the Optimal Parameters When Using an Ultrasonic Probe to Measure Cavern Geometry Where a Metal Borehole Pipe Is Present
Authors: Kubacka Tomasz , Dinh Chau Nguyen
Published: June 2021

In order to determine the optimal parameters when using an ultrasonic probe to measure cavern geometry when a metal borehole pipe is present, an investigation was firstly carried out on influence of a vertical metal plates with a thickness from 1 mm to 15 mm immersed in water on transmitted and reflected ultrasonic waves. The results obtained will be used as an indicator for the measurement of underground geometry in which the ultrasonic probe is placed inside a metal pipe lining a borehole. These studies were performed both by experiment and computer simulation. The results show that the wavelength of the incident ultrasonic signals should be equal to half the thickness of the metal plate or an integer times smaller than this thickness. When the thickness of the barrier is unknown, an ultrasonic signal with linear frequency modulation (LFM) should be used. Due to the reverberation of the ultrasonic waves inside the pipe for caverns filled with water, the distance from the transducer to the cavern wall can be measured if it is longer than three times of the pipe diameter. Frequency analysis of both the reflected and the transmitted waves enables an optimal frequency of the incident ultrasonic wave to be selected, which can be used in the measurement of cavern geometry in conditions in which the ultrasonic probe is inside a metal pipe.
A Modified Model Predictive Torque Control with Parameters Robustness Improvement for PMSM of Electric Vehicles
Authors: Gao Siyu , Wei Yanjun , Zhang Di , Qi Hanhong , Wei Yao
Published: June 2021

Model predictive torque control with duty cycle control (MPTC-DCC) is widely used in motor drive systems because of its low torque ripple and good steady-state performance. However, the selection of the optimal voltage vector and the calculation of the duration are extremely dependent on the accuracy of the motor parameters. In view of this situation, A modified MPTC-DCC is proposed in this paper. According to the variation of error between the measured value and the predicted value, the motor parameters are calculated in real-time. Meanwhile, Model reference adaptive control (MRAC) is adopted in the speed loop to eliminate the disturbance caused by the ripple of real-time update parameters, through which the disturbance caused by parameter mismatch is suppressed effectively. The simulation and experiment are carried out on MATLAB / Simulink software and dSPACE experimental platform, which corroborate the principle analysis and the correctness of the method.
The Response of Soil pH and Exchangeable Al to Alum and Lime Amendments
Authors: Gillespie Christopher Jorelle , Antonangelo João Arthur , Zhang Hailin
Published: June 2021

Intensive cultivation and unprecedented utilization of ammoniacal fertilizer has accelerated soil acidification in the southern Great Plains and many other parts of the world. During a two-year study that evaluated the impact of soil pH and aluminum (Al) toxicity on winter wheat yield potential, we observed a variance in the edaphic responses of the two study sites (Stillwater and Chickasha) to two soil amendments, Alum [Al2(SO4)3] and lime [Ca(OH)2]. We found that AlKCl values at Stillwater were 223% and 150% higher than Chickasha during Year 1 and Year 2, respectively, with similar soil pH. Additionally, Alsat values at Stillwater were 30.6% and 24.9% higher than Chickasha during Year 1 and Year 2, respectively. Surprisingly, when treated as a bivariate of Alsat, soil buffer indices differed in graphical structure. While Chickasha was identified with a cubic polynomial (p < 0.0001), Stillwater was characterized by linear regression (p < 0.0001). We have reason to believe that this divergence in edaphic response might be attributed to the organically bound Al, dissolved organic carbon (DOC), spatio-temporal variance, and adsorption reactions regulated by the solubility of Al(OH)+2 species in acidic soils.
Economic and Environmental Consequences of the ECJ Genome Editing Judgment in Agriculture
Authors: Gocht Alexander , Consmüller Nicola , Thom Ferike , Grethe Harald
Published: June 2021

Genome-edited crops are on the verge of being placed on the market and their agricultural and food products will thus be internationally traded soon. National regulations, however, diverge regarding the classification of genome-edited crops. Major countries such as the US and Brazil do not specifically regulate genome-edited crops, while in the European Union, they fall under GMO legislation, according to the European Court of Justice (ECJ). As it is in some cases impossible to analytically distinguish between products from genome-edited plants and those from non-genome-edited plants, EU importers may fear the risk of violating EU legislation. They may choose not to import any agricultural and food products based on crops for which genome-edited varieties are available. Therefore, crop products of which the EU is currently a net importer would become more expensive in the EU, and production would intensify. Furthermore, an intense substitution of products covered and not covered by genome editing would occur in consumption, production, and trade. We analyzed the effects of such a cease of EU imports for cereals and soy in the EU agricultural sector with the comparative static agricultural sector equilibrium model CAPRI. Our results indicate dramatic effects on agricultural and food prices as well as on farm income. The intensification of EU agriculture may result in negative net environmental effects in the EU as well as in an increase in global greenhouse gas (GHG) emissions. This suggests that trade effects should be considered when developing domestic regulation for genome-edited crops.
Some Emerging Opportunities of Nanotechnology Development for Soilless and Microgreen Farming
Authors: Maluin Farhatun Najat , Hussein Mohd Zobir , Nik Ibrahim Nik Nor Liyana , Wayayok Aimrun , Hashim Norhayati
Published: June 2021

Global food demand has increased in tandem with the world’s growing population, prompting calls for a new sustainable agricultural method. The scarcity of fertile soil and the world’s agricultural land have also become major concerns. Soilless and microgreen farming combined with nanotechnology may provide a revolutionary solution as well as a more sustainable and productive alternative to conventional farming. In this review, we look at the potential of nanotechnology in soilless and microgreen farming. The available but limited nanotechnology approaches in soilless farming include: (1) Nutrients nanoparticles to minimize nutrient losses and improve nutrient uptake and bioavailability in crops; (2) nano-sensing to provide real-time detection of p H, temperature, as well as quantifying the amount of the nutrient, allowing desired conditions control; and (3) incorporation of nanoparticles to improve the quality of substrate culture as crop cultivation growing medium. Meanwhile, potential nanotechnology applications in soilless and microgreen farming include: (1) Plant trait improvement against environmental disease and stress through nanomaterial application; (2) plant nanobionics to alter or improve the function of the plant tissue or organelle; and (3) extending the shelf life of microgreens by impregnating nanoparticles on the packaging or other preservation method.
Disaggregating the Value of Conservation Agriculture to Inform Smallholder Transition to Sustainable Farming: A Mexican Case Study
Authors: Monjardino Marta , López-Ridaura Santiago , Van Loon Jelle , Mottaleb Khondoker Abdul , Kruseman Gideon , Zepeda Adaír , Hernández Erick Ortiz , Burgueño Juan , Singh Ravi Gopal , Govaerts Bram , Erenstein Olaf
Published: June 2021

Conservation Agriculture (CA) is promoted by research and development (R&D) agencies to sustainably intensify agricultural systems with the goals of improving food security and livelihoods and adapting food systems to global climate change. Despite the many benefits of CA, there are few farmers around the world that have simultaneously implemented all facets of the strategy. In part, this reflects the challenges in applying, adapting, and understanding this complex and multi-dimensional agricultural innovation in the context of diverse farming systems. In this paper, we applied an integrated framework that combines bioeconomic simulation, risk analysis, adoption theory, and impact assessment to investigate how various combinations of CA components (no-tillage, soil cover, crop diversification, plus growing a new crop or variety) performed over a 10-year period in representative farms in a central Mexican case study. We found significant differences in profit, net value, downside risk, and risk-aversion cost between double-component scenarios (and improved CA to a lesser extent) and all other scenarios, which suggested that disaggregating CA into smaller component packages could increase farmer adoption in risky contexts. Our findings provided valuable insights on CA feasibility and could help establish policy and reporting metrics. The study highlighted the need for employing a range of research tools to understand the relative value of agricultural innovations and to identify and reduce trade-offs and uncertainty in farming systems.
The Effect of the Foliar Application of Biostimulants and Fertilisers on the Growth and Physiological Parameters of Maiden Apple Trees Cultivated with Limited Mineral Fertilisation
Authors: Świerczyński Sławomir , Antonowicz Agnieszka , Bykowska Joanna
Published: June 2021

The article describes an experiment conducted on maiden apple trees of selected cultivars growing in a nursery. The aim of the study was to investigate how the foliar application of four biostimulants (Aminoplant 0.4%, Biamino Plant 0.2%, Bispeed 0.2% and Fylloton 0.4%) and two fertilisers (Basfoliar 6-12-6 and Basfoliar 12-4-6+S both at a concentration of 0.5%) affected the growth of the trees cultivated with the mineral fertilisation reduced by half. The plants were sprayed with the preparations and fertilisers four times at three-week intervals, between late May and late July. Selected parameters of the plants’ physiological processes were also assessed: net photosynthetic rate (Pn), transpiration rate (E), stomatal conductance (C) and intracellular CO2 (I). The foliar application of all the biostimulants and fertilisers significantly improved the growth of the maiden apple trees. The lowest values of the plant growth parameters were noted in the control combination, which was treated with a full dose of mineral fertiliser. The foliar spraying of the apple trees particularly improved the total length and number of side shoots and the fresh weight of maiden apple trees. The apple tree cultivars differed in their growth parameters, and the influence of individual foliar treatments was not conclusive. The foliar application of selected preparations intensified the leaf transpiration coefficient and the internal concentration of carbon dioxide, but it did not increase the net photosynthesis intensity or stomatal conductance.
Uptake and Utilization of Nitrogen from Organic Fertilizers Influenced by Different Doses of Copper
Authors: Kuziemska Beata , Trębicka Joanna , Wysokinski Andrzej
Published: June 2021

Copper is a microelement involved in the metabolism of nitrogen compounds in plants. Good utilization of nitrogen from soil and fertilizers by plants requires an adequate supply of copper. The aim of the study was to determine the effect of increasing levels of copper (100, 200, and 300 mg Cu·kg−1 of soil) applied together with various organic fertilizers (cattle manure, chicken manure, and spent mushroom substrate) on nitrogen content and uptake by cocksfoot (Dactylis glomerata L.) and the coefficient of nitrogen utilization from organic fertilizers. The pot experiment was carried out in three growing seasons (May–September) in greenhouse, and in this cocksfoot was grown and harvested four cuts in each year. Copper and organic fertilizers were applied once in the first year before sowing cocksfoot, and the after-effect was investigated in the second and third years. Application of different amounts of copper did not influence the nitrogen content in the biomass of cocksfoot. At the same time, soil application of this micronutrient in the amount of 100 mg Cu∙kg−1 of soil caused an increase in nitrogen uptake in the biomass of cocksfoot. Application of 100 and 200 mg Cu·kg−1 of soil caused an increase in the coefficient of nitrogen utilization from the organic fertilizers, which was highest effect in the case of cattle manure. All of the organic materials used increased the content of nitrogen and its uptake by cocksfoot, but the greatest effect was noted following application of chicken manure. The study showed no synergistic or antagonistic relationships between copper and nitrogen.
Characterization of Extended-Spectrum Cephalosporin (ESC) Resistance in Salmonella Isolated from Chicken and Identification of High Frequency Transfer of blaCMY-2 Gene Harboring Plasmid In Vitro and In Vivo
Authors: Kwon Bo-Ram , Wei Bai , Cha Se-Yeoun , Shang Ke , Zhang Jun-Feng , Jang Hyung-Kwan , Kang Min
Published: June 2021

A total of 136 Salmonella isolates from chicken feces and meat samples of the top 12 integrated chicken production companies throughout Korea were collected. Among the 17 ESC-resistant Salmonella; blaCTX-M-15 was the most prevalent gene and two strains carried blaTEM-1/blaCTX-M-15 and blaCMY-2, respectively. The transferable blaCTX-M-15 gene was carried by IncFII plasmid in three isolates and the blaCMY-2 gene carried by IncI1 plasmid in one isolate. blaCMY-2 gene-harboring strain was selected as the donor based on the high frequency of blaCMY-2 gene transfer in vitro and its transfer frequencies were determined at 10−3 transconjugants per recipient. The transfer of blaCMY-2 gene-harboring plasmid derived from chicken isolate into a human pathogen; enteroinvasive Escherichia coli (EIEC), presented in mouse intestine with about 10−1 transfer frequency without selective pressure. From the competition experiment; blaCMY-2 gene-harboring transconjugant showed variable fitness burden depends on the parent strains. Our study demonstrated direct evidence that the blaCMY-2 gene harboring Salmonella from chicken could frequently transfer its ESC-resistant gene to E. coli in a mouse intestine without antimicrobial pressure; resulting in the emergence of multidrug resistance in potentially virulent EIEC isolates of significance to human health; which can increase the risk of therapeutic inadequacy or failures.
Beehive Products as Antibacterial Agents: A Review
Authors: Nader Rita Abou , Mackieh Rawan , Wehbe Rim , El Obeid Dany , Sabatier Jean Marc , Fajloun Ziad
Published: June 2021

Honeybees are one of the most marvelous and economically beneficial insects. As pollinators, they play a vital role in every aspect of the ecosystem. Beehive products have been used for thousands of years in many cultures for the treatment of various diseases. Their healing properties have been documented in many religious texts like the Noble Quran and the Holy Bible. Honey, bee venom, propolis, pollen and royal jelly all demonstrated a richness in their bioactive compounds which make them effective against a variety of bacterial strains. Furthermore, many studies showed that honey and bee venom work as powerful antibacterial agents against a wide range of bacteria including life-threatening bacteria. Several reports documented the biological activities of honeybee products but none of them emphasized on the antibacterial activity of all beehive products. Therefore, this review aims to highlight the antibacterial activity of honey, bee venom, propolis, pollen and royal jelly, that are produced by honeybees.
Finding a Balance in the Vaginal Microbiome: How Do We Treat and Prevent the Occurrence of Bacterial Vaginosis?
Authors: Joseph Rebecca Jane , Ser Hooi-Leng , Kuai Yi-He , Tan Loh Teng-Hern , Arasoo Valliammai Jayanthi Thirunavuk , Letchumanan Vengadesh , Wang Lijing , Pusparajah Priyia , Goh Bey-Hing , Ab Mutalib Nurul-Syakima , Chan Kok-Gan , Lee Learn-Han
Published: June 2021

Bacterial vaginosis (BV) has been reported in one-third of women worldwide at different life stages, due to the complex balance in the ecology of the vaginal microbiota. It is a common cause of abnormal vaginal discharge and is associated with other health issues. Since the first description of anaerobic microbes associated with BV like Gardnerella vaginalis in the 1950s, researchers have stepped up the game by incorporating advanced molecular tools to monitor and evaluate the extent of dysbiosis within the vaginal microbiome, particularly on how specific microbial population changes compared to a healthy state. Moreover, treatment failure and BV recurrence rate remain high despite the standard antibiotic treatment. Consequently, researchers have been probing into alternative or adjunct treatments, including probiotics or even vaginal microbiota transplants, to ensure successful treatment outcomes and reduce the colonization by pathogenic microbes of the female reproductive tract. The current review summarizes the latest findings in probiotics use for BV and explores the potential of vaginal microbiota transplants in restoring vaginal health.
Rapid and Accurate Detection of Escherichia coli and Klebsiella pneumoniae Strains Susceptible/Resistant to Cotrimoxazole through Evaluation of Cell Elongation
Authors: López Isidoro , Otero Fátima , Guillén Rebeca , Fernández María del Carmen , Bou Germán , Gosálvez Jaime , Fernández José Luis
Published: June 2021

Trimethoprim-sulfamethoxazole is a well-known antibiotic that inhibits folic acid synthesis, a topic of renewed interest. Since resistant strains are increasingly more common, an early and accurate discrimination of susceptibility may assure confident therapy. Two morphological assays were performed in Escherichia coli (n = 50; 27 non-susceptible) and Klebsiella pneumoniae (n = 52; 18 non-susceptible). First, the strains were incubated with the CLSI breakpoint of cotrimoxazole for 150 min, which induced cell lengthening in the susceptible strains. Second, the bacteria were incubated with mitomycin C (MMC) (0.5 mg/L) for 120 min to induce a SOS-linked cell enlargement higher than that obtained by cotrimoxazole alone. When cotrimoxazole was added 30 min before MMC, the inhibition of folic acid synthesis in the susceptible strain resulted in the suppression of MMC-induced extra elongation. In the non-susceptible strains, folic acid synthesis continued despite the antibiotic, so that the MMC-induced extra cell lengthening could not be impeded. Whereas the first assay resulted in five false negatives and four false positives of resistance, the results of the second assay matched those of the conventional antibiogram. This simple morphological procedure is performed in 2 h and 45 min and may allow a rapid selection of useful and relatively inexpensive therapy, thereby preserving the newer broad-spectrum antibiotics.
Eupatilin Impacts on the Progression of Colon Cancer by Mitochondria Dysfunction and Oxidative Stress
Authors: Lee Minkyeong , Yang Changwon , Song Gwonhwa , Lim Whasun
Published: June 2021

Colon cancer is one of the most frequently diagnosed cancer types. Some colon cancer cases resist standard anticancer drugs. Therefore, many studies have focused on developing therapeutic supplements using natural products with low side effects and broad physiological activity. Eupatilin is a flavonoid that is mainly extracted from artemisia and promotes apoptosis in numerous cancer types. However, since the current understanding of its physiological mechanisms on colon cancer cells is insufficient, we investigated how eupatilin affects the growth of two colon cancer cell lines, namely HCT116 and HT29. Our results showed that eupatilin inhibits cell viability and induces apoptosis accompanied by mitochondrial depolarization. It also induces oxidative stress in colon cancer cells and regulates the expression of proteins involved in the endoplasmic reticulum stress and autophagic process. Moreover, eupatilin may target the PI3K/AKT and mitogen-activated protein kinase (MAPK) signaling pathways in colon cancer cells. It also prevents colon cancer cell invasion. Furthermore, eupatilin has a synergistic effect with 5-fluorouracil (5-FU; a standard anticancer drug) on 5-FU-resistant HCT116 cells. These results suggest that eupatilin can be developed as an adjuvant to enhance traditional anticancer drugs in colon cancer.
Redox Biomarker Baseline Levels in Cattle Tissues and Their Relationships with Meat Quality
Authors: Skaperda Zoi , Argyriadou Angeliki , Nechalioti Paraskevi Maria , Alvanou Maria , Makri Sotiria , Bouroutzika Efterpi , Kyriazis Ioannis D. , Tekos Fotios , Veskoukis Aristidis S. , Kallitsis Theodoros , Mesnage Robin , Arsenos Georgios , Kouretas Demetrios
Published: June 2021

Cattle breeds or crossbreds with high productivity traits have been developed to meet a growing demand for food. When intensive farming practices are followed, animals face several challenges which can result in poor performance, compromised welfare and the reduced quality of their products. Our study aims to highlight the resting values of the physiological oxidative stress that three cattle breeds exhibit, and their potential relationship with meat quality. For this purpose, we determined the levels of five common redox biomarkers (glutathione (GSH), catalase (CAT), total antioxidant capacity (TAC), thiobarbituric reactive substances (TBARS) and protein carbonyls (CARBS)) in the tissues of three commonly used beef cattle breeds (Charolais (CHA), Limousin (LIM) and Simmental (SIM)) and their association with specific meat quality traits that depend on color, pH and texture. The results revealed that LIM cattle breed animals have elevated intrinsic antioxidant defense systems in comparison to CHA and SIM cattle breed animals. In addition, the meat quality parameters were associated with the redox biomarkers. We propose that the determination of specific antioxidant parameters in the blood might be used as potential biomarkers to predict meat quality. This would allow farmers to nutritionally intervene to improve the quality of their products.
Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol
Authors: Nowaczyk Magdalena , Malcher Agnieszka , Zimna Agnieszka , Łabędź Wojciech , Kubaszewski Łukasz , Barczak Wojciech , Rubiś Błażej , Rozwadowska Natalia , Kurpisz Maciej
Published: June 2021

The aim of the study was to modify human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) and demonstrate the optimal cell preparation protocol for application in post-infarction hearts. We used conditioned SkMDS/PC culture medium with α-phenyl-N-tert-butyl nitrone (PBN). SkMDS/PCs were cultured under hypoxic conditions and the results were compared to the standard ones. We observed a significant increase of CD-56 positive phenotypic marker the ability to form functional myotubes, increase in the proportion of young cells in cell primary suspensions, and a decrease in the percentage of apoptotic cells among PBN-conditioned cells in normoxia an hypoxia. We also observed significantly higher levels of SOD3 expression; maintained expression of SOD1, SOD2, and CAT; a higher level of BCL2 gene expression; and a rather significant decrease in Hsp70 gene expression in PBN-conditioned SkMDS/PCs compared to the WT population under hypoxic conditions. In addition, significant increase of myogenic genes expression was observed after PBN addition to culture medium, compared to WT population under hypoxia. Interestingly, PBN addition significantly increased the lengths of telomeres under hypoxia. Based on the data obtained, we can postulate that PBN conditioning of human SkMDS/PCs could be a promising step in improving myogenic cell preparation protocol for pro-regenerative treatment of post-infarction hearts.
Application of a Two-Stage Steam Jet Injector Unit for Latent Heat Recovery of a Marine Steam Turbine Propulsion Plant
Authors: Grzesiak Szymon , Adamkiewicz Andrzej
Published: June 2021

The paper presents the results of the numerical research of the steam jet injector applications for the regenerative feed water heating systems of marine steam turbine propulsion plants. The analysis shows that the use of a single injector for a single heat exchanger results in a relative increase in the thermal efficiency of the plant by 0.6–0.9%. The analysis also indicates the legitimacy of the usage of multistage feed water heating systems, which would enable the operating parameters optimization of the injectors. The obtained steam pressure up to the value of 1.8 barA allows for the heating of the feed water up to 110 °C. For higher degrees of feed water heating in the heat exchangers, it is necessary to supply heating steam of higher pressure. Therefore, the usage of two-stage steam jet injector units was considered advisable for the analyses.