bluuebunny/crossref_metadata_2025 huggingface dataset:
Abstract PR07: SETD1B mutations confer apoptosis resistance and BCL2 independence in B-cell lymphoma
Authors: Portelinha Ana , Wang Shenqiu , Parsa Sara , Jiang Man , Mohanty Sagarajit , Sharma Soumya , Arya Neeraj , Tavana Omid , Wen Jiayu , Fitzgibbon Jude , Dogan Ahmet , Younes Ana , Melnick Ari M. , Wendel Hans-Guido
Published: June 2024

The translocation t(14;18) activates BCL2 and is considered the initiating genetic lesion in over ninety percent of follicular lymphomas (FL). Surprisingly, FL patients respond poorly to clinically approved inhibitor, venetoclax. The mechanisms underlying BCL2 independence in FL are poorly understood, and it has been speculated that epigenetic rewiring of cell death programs may play a role. We performed separate genome-wide CRISPR/Cas9 screens to explore the mechanisms of resistance to Venetoclax and an experimental MCL-1 inhibitor (S63845) and unveiled the histone lysine methyltransferase SETD1B (KMT2G) as an unexpected mechanism of resistance to both inhibitors in lymphoma. We show that mutations and deletions affecting SETD1B occur in 7% of FLs and 16% of diffuse large B cell lymphomas (DLBCL). In vivo, SETD1B acts as a tumor suppressor gene and cooperates with the related histone-lysine N-methyltransferase 2D (KMT2D) gene leading to a more aggressive disease presentation. This oncogenic cooperation is reflected in the genomes of human lymphoma, where SETD1B mutations typically co-occur with KMT2D mutations. Mechanistically, SETD1B is required to express pro-apoptotic BCL2 family proteins like BIM and BIK. Inhibitors of the KDM5 histone H3K4 de-methylase restore the expression of BIM and BIK, venetoclax sensitivity and lead to synergistic drug effects in SETD1B deficient lymphoma xenografts. Our results highlight a role for SETD1B in the epigenetic regulation of cell death proteins in B cell lymphomas, connecting the loss of SETD1B to lymphoma development and reduced sensitivity to venetoclax.

Citation Format: Ana Portelinha, Shenqiu Wang, Sara Parsa, Man Jiang, Sagarajit Mohanty, Soumya Sharma, Neeraj Arya, Omid Tavana, Jiayu Wen, Jude Fitzgibbon, Ahmet Dogan, Ana Younes, Ari M. Melnick, Hans-Guido Wendel. SETD1B mutations confer apoptosis resistance and BCL2 independence in B-cell lymphoma [abstract]. In: Proceedings of the Fourth AACR International Meeting on Advances in Malignant Lymphoma: Maximizing the Basic-Translational Interface for Clinical Application; 2024 Jun 19-22; Philadelphia, PA. Philadelphia (PA): AACR; Blood Cancer Discov 2024;5(3_Suppl):Abstract nr PR07.
Pharmacist-Led Medication Management in Acute Geriatric Medicine and Its Associations with Rehospitalizations: A Cohort Study
Authors: Freitag Mathias , Franzen Julia , Just Katja Susanne , Eisert Albrecht , Bollheimer Leo Cornelius , Laurentius Thea
Published: June 2024

Introduction: Hospitalization and discharge in older patients are critical and clinical pharmacists have shown to ameliorate risks. Our objective was to assess their benefit as part of the geriatric team regarding rehospitalizations and related outcomes after discharge focusing on general practitioners’ decision to continue or change discharge medication (GPD). Methods: Prospective implementation study with 6-month follow-up in an acute geriatric clinic. Patients ≥70 years with comorbidities, impairments, and a current drug therapy were consecutively assigned to three groups: control group (CG), implementation group (IG), and wash-out group (WG). CG only received medication reconciliation (MR) at admission; IG and their hospital physicians received a pharmaceutical counseling and medication management; during WG, pharmaceutical counseling except for MR was discontinued. We used a negative-binomial model to calculate rehospitalizations and days spent at home as well as a recurrent events survival model to investigate recurrent rehospitalizations. Results: One hundred thirty-two patients (mean age 82 years, 76 women [57.6%]) finished the project. In most of the models for rehospitalizations, a positive GPD led to fewer events. We also found an effect of pharmaceutical counseling on rehospitalizations and recurrent rehospitalizations in the CG versus WG but not in the CG versus IG models. 95.3% of medication recommendations by the pharmacist in the clinic setting were accepted. While the number of positive GPDs in CG was low (38%), pharmaceutical counseling directly to the GP in IG led to a higher number of positive GPDs (60%). Discussion: Although rehospitalizations were not directly reduced by our intervention in the CG versus IG, the pharmacist’s acceptance rate in the hospital was very high and a positive GPD led to fewer rehospitalization in most models.
Diversity of nocturnal Cerambycidae (Coleoptera: Chrysomeloidea) from seven localities in Michoacán, Mexico, with description of a new species
Authors: ALTAMIRANO JANETH , PÉREZ-FLORES OSCAR , ESCALANTE-JIMÉNEZ ANA LETICIA , JURADO-VARGAS CESAR MARCO
Published: June 2024

We used a long-time light trap for capturing nocturnal beetles. The specimens collected belong to 24 species, 21 genera, 11 tribes, and three subfamilies of the family Cerambycidae. Of these, Gnaphalodes trachyderoides Thomson, 1861; Tylcus hartwegii (White, 1855); Anelaphus piceus (Chemsak, 1962); Metironeus hesperus Chemsak, 1991; Pseudoperiboeum subarmatum Linsley, 1935; Neocompsa puncticollis asperula (Bates, 1885); Compsibidion vanum (Thomson, 1867); Canidia spinicornis (Bates, 1881); Lepturges (Lepturges) angulatus (LeConte, 1852); Sternidius naeviicornis (Bates, 1885); Oreodera noguerai McCarty, 2001; Eupogonius arizonensis Knull, 1954; Lypsimena fuscata Haldeman, 1847; Arhopalus asperatus (LeConte, 1859); and Arhopalus montanus (LeConte, 1873) represent new state records. One new species is described, Adetus michoacanensis sp. nov. (Lamiinae: Apomecynini).
Review of the ground beetle subgenus Micronialoe from Korea, with a description of a new species (Coleoptera: Carabidae: Pterostichus)
Authors: KIM DOOYOUNG , SUH SANG JAE
Published: June 2024

Micronialoe (Pterostichini: Pterostichus) is a poorly known subgenus of the ground beetle genus Pterostichus, which includes three species from the Korean Peninsula and adjacent islands. Here, a new species, Pterostichus (Micronialoe) spinulosus sp. nov., was identified among the specimens collected from Mt. Biseulsan and Mt. Choejeongsan, Daegu-si, Korea. Along with the description of the new species, all Korean species are redescribed, and the subgenus is re-diagnosed. The new species is morphologically allied to P. (M.) bifoveolatus, thus, the intraspecific variation of the new species is examined and valid characters for distinguishing species are discussed. A key for the identification of the species of the subgenus is also provided.
First record of the family Azotidae from Ecuador, with a description of a new species of Ablerus Howard (Hymenoptera: Chalcidoidea: Azotidae) from the Galapagos Islands
Authors: AVENDAÑO JOSÉ M. , PICÓN-RENTERÍA PATRICIO , KRESSLEIN ROBERT L. , HERRERA HENRI W.
Published: June 2024

Azotidae (Hymenoptera: Chalcidoidea) is a small family (1 genus, 92 species) comprised primarily of hyperparasitoids of species of Hymenoptera that target whiteflies and scale insects. In the present study, we provide the first record of the genus Ablerus Howard, 1898 (=Azotus Howard 1898) from the Galapagos Islands, based on material collected in a survey of Hymenoptera across the archipelago’s main islands. One new species, Ablerus byroni sp. n. is described and illustrated.
Regression–Based Diagnostic Models for Early Lung Cancer Integrating Conventional Indicators with Tumor Markers
Authors: Zhou Shufang , Ge Xiaojun , Yang Zhifang , Zeng Fei
Published: June 2024

The aim of this research was to develop a lung cancer diagnostic and predictive model that integrates traditional laboratory indicators with tumor markers. This model is intended to facilitate early screening and assist in the process of identifying or detecting lung cancer through a cost-effective, rapid, and convenient approach, ultimately enhancing the early detection rate of lung cancer. A retrospective study was conducted on 66 patients diagnosed with lung cancer and 159 patients with benign pulmonary conditions. Data including general clinical information, conventional laboratory test results, and tumor marker levels were collected. Data analysis was conducted using SPSS 26.0 (Statistical Product and Service Solutions 26.0). The lung cancer diagnosis and prediction model is created using a composite index established through binary logistic regression. The combined diagnostic prediction models, incorporating both traditional indicators and tumor markers, demonstrated a greater area under the curve (AUC) when compared to the diagnostic prediction model based solely on tumor markers and their combination testing. The values of cut-off point, AUC, accuracy, sensitivity, specificity, positive and negative detection rate and accuracy rate are 0.1805, 0.959, 86.67%, 0.955, 0.830, 95.45%, 83.02% and 89.33 respectively and it is shown that the combined diagnostic model display notable efficacy and clinical relevance in aiding the early diagnosis of lung cancer.
Noise Pollution Exposure Among Nurses in a Public Hospital in Palestine
Authors: Elafifi May , Elawady Mohamed , Alrifai Ayesha
Published: June 2024

Noise pollution affects our health adversely. At hospital, noise can harm health care professionals and patients too. It also affects the performance of the whole staff and therefore, the quality of the delivered services. This study aimed to assess noise levels nurses exposed to and to determine the sources of noise and nurses' complaints about it. A quantitative, descriptive cross sectional study was used. Noise levels at different departments in Palestine Medical Complex (PMC) were measured using an integrating sound level meter. A total of 180 nurses participated in the interviewing questionnaire. The results of the study showed that main sources of noise were: the loud arguments occurred between the health care staff and the relatives/visitors of the patients, loud conversations, the construction work and the renovation in the hospital, patients' crying/shouting, the medical equipment and the staff shouting. The main complaints were discomfort, bad communication, headache, fatigue, stress, irritability, low concentration, inattention, memory problems and noise effect on work efficiency. In most departments, morning shift was the noisiest shift and nurses (61.1%) considered work environment stressful and uncomfortable. Noise levels in all departments exceeded what is recommended by WHO with an average of 64.48 dBA. The minimum value of LAeq was 54.8 dBA in one CCU and the maximum one was 73.5 dBA in NICU. Conclusion: Different noise sources at PMC and many noise complaints were reported by nurses. Noise levels in different departments were above that recommended by WHO. The educational intervention should target nurses and the whole health care staff in order to increase their awareness about noise and to modify their behaviors to reduce it, as well as the effective management of medical devices and machines, repairing devices and physical space arrangement.
Fermionic and Bosonic Partition Functions at Imaginary Chemical Potential as Bloch Functions
Authors: Filothodoros Evangelos Georgiou
Published: June 2024

In this work it is pointed out that the phase transitions of the d+1 Gross-Neveu (fermionic) and CPN−1 (bosonic) models at finite temperature and imaginary chemical potential can be mapped to transformations of Hubbard-like regular hexagonal to square lattice with the intermediate steps to be specific surfaces (irregular hexagonal kind) with an ordered construction based on the even indexed Bloch-Wigner-Ramakrishnan polylogarithm function. The zeros and extrema of the Clausen Cld(θ) function play an important role to the analysis since they allow us not only to study the fermionic and bosonic theories and their phase transitions but also the possibility to explore the existence of conductors arising from the correspondence between the partition functions of the two models and the Bloch and Wannier functions that play a crucial role in the tight-binding approximation in solid state physics. The main aim of this work is not only to unveil the relevance of the canonical partition functions of a fermionic and a bosonic model to Bloch states by using an imaginary chemical potential but also to examine the overlap between two Bloch wave-functions that differ by a lattice momentum that calculates the momentum transfer of a Bloch wave during the interaction with a lattice point of a hexagonal construction.
Epidemiological, Clinical and Radiological Profiles of Post-stroke Aphasia in Neurology in Abidjan from April to September 2022
Authors: Roxane Beuseize , Léonard Kouassi , Stéphane Abbé , Samuel Yeo , Tanya Essoin-De , Yves Broh , Delors Offoumou , Imaila Diakité , Mariam Doumbia-Ouattara
Published: June 2024

Background and purpose: Aphasia is a language disorder that frequently occurs after a cerebrovascular accident. This prospective descriptive study aimed to describe the epidemiological, clinical and radiological profiles of post-stroke aphasia. Materials and method: Patients presenting aphasia after a stroke documented by brain imaging, hospitalized from April to September 2022 in the neurology departments of the Cocody and Treichville University Hospitals were included. Aphasia was screened using the Language Screening Test (LAST). Epidemiological, clinical and radiological data were collected through clinical observation. Results: Of the 217 stroke patients admitted to neurology during the period, 32 presented aphasia (14.7%). The average age was 56 years, with a male predominance (M/F sex ratio of 2.2). The majority of patients had primary or no schooling (87.5%). Arterial hypertension was the most frequent cardiovascular risk factor (50%) and hemiplegia, the most common physical sign (93.8%). The average LAST score for aphasic patients was 5.3 out of 15. We observed that naming and repetition were the most affected components of speaking, with scores below normal in 87.5%. and 88% of cases. Regarding oral comprehension, order execution was the component most affected for 75.1% of patients. In the acute phase of stroke, 50% of cases of aphasia were severe. Radiologically, 75% of patients presented an ischemic stroke with the predilection of the middle cerebral artery. Conclusion: this study highlights the importance of early detection of aphasia in post-stroke patients and rapid treatment to optimize their linguistic recovery.
Research on Knitted Garment Design and Innovation Countermeasures--Tongxiang Puyuan Knitwear Industry as an Example
Authors: Yingzhe Zhang
Published: June 2024

As an important knitted garment production area in China, Tongxiang knitted garment market has a good production base. However, with the adjustment of supply and demand and changes in consumer demand, the existing knitted garment industry needs to adapt to the development of the times to carry out the corresponding industrial upgrading. This paper focuses on the existing knitted garment industry problems, such as: lack of innovation in knitted garment design, lack of brand competitiveness in the knitted garment market and inaccurate industrial positioning. Try to put forward to the use of design innovation, increase research and development efforts to improve the comprehensive ability of knitted garment enterprises; training of professional knitted garment design talent, and increase investment in science and technology so as to improve product quality and market taste; will be the neighboring regions of the garment industry organically combined together to drive the upgrading of the development of industrial proposals and countermeasures. Design and innovation as the driving force, better development of knitted garment industry in Tongxiang City.
Honeybee Floral Diversity of Gemachis Forest, West Hararghe Zone, Oromia Regional State, Ethiopia
Authors: Dugda Dema , Addi Admassu
Published: June 2024

The study was conducted in Gemachis forest from October 2019 to November 2020, which is an important area of biodiversity conservation, tourism attraction and carbon sequestration for modulating climate change. The forest is also very important for honey production and other non-timber forest products. The study was designed with the objective of assessing honey bee floral diversity of the forest. For the inventory of bee forages a transect line were made in three selected altitudinal strata. Honey samples also were obtained from the three agro ecologies of the forest: Arer (high altitude), Chafe kebene (medium altitude) and Sororo (low altitude). The pollen spectrum of the honey was analyzed to determine the botanical and geographical origin of the honey. According to inventory of the honeybee flora, forty-eight (48) bee plant species were identified; belonging to 33 families, of which Fabaceae, Roseaceae and Verbenaceae were the most frequent families in the study area. Among these bee forages 54.1% were shrub 16.6% were trees and 29.1% were herbs. From identified bee forages, Solanium spp, Andropogon abyssinica, Guizotia spp and Hypoestes forskaolii were the most abundant bee flora species in the study area. The pollen analysis of honey revealed that, two types of monofloral honey types were identified in the area which includes Guizotia spp and Eucalyptus globulus accounting for 74.9% and 54.9% pollen frequency respectively. Thus, beekeepers should conserve the forest for sustainable honey production since the forest is endowed with good honeybee plant diversity.
Assessing the Safety and Efficacy of Spinal Anesthesia in Preterm and Term Neonates: A Multicenter Experience
Authors: Baffah Adamu , Ballah Abubakar , Bidemi Yahaya , Mohammed Jika , Njidda Gyadale , Mabong Nwokorie , Aminu Galadima , Ali Hassan , Joda Ibrahim , Maryam Abdullahi , Abdullahi Adam , Franklin Andibanbang
Published: June 2024

Background: Worldwide, the incidence of premature birth is increasing with its attending congenital anomalies, so complex surgical interventions to manage these congenital anomalies or complications are usually performed at early days of life. Neonates are vulnerable to apnea, and congenital cardiopulmonary problems and they have higher morbidity and mortality compared to older children and adults etc. In the preterm neonate, the risk of bronchopulmonary dysplasia and apnoea make their management complex and this is further compounded by their smaller size. Objective of study: The aim of the study was to determine the safety and efficacy of spinal anesthesia among neonates. Methodology: The study is a prospective study conducted in three tertiary health Institutions in northeast Nigeria. It includes all neonates scheduled for surgery amenable under SA. Sociodemographic variables, Haemodynamic variables, duration of surgery, and complications observed were all recorded. Results: Spinal anaesthesia SA was done in 230 patients, 90% of the patients were term, and only 10% were preterm. The majority were male with a mean weight of 3002 ± 660 grams and a mean age of 14.51±7.28 days. SA was successful in 93% of the patients in the first attempt. Heart rate, mean arterial pressure and oxygen saturation remained stable all through the duration of surgery. About 6% of the patients were converted to General Anaesthesia GA on account of prolonged surgery. The highest level of sympathetic block was recorded at the level of T4 while the lowest was at T6. Herniotomy was the most common surgery performed. The surgery lasted an average of 72 ± 28 minutes. No complication was recorded. Conclusion: Spinal anaesthesia provides a safe and reliable option for neonates who may require surgery early in life to improve their chances of survival. It serves as an alternative to GA with enhanced haemodynamic stability in this fragile population.
Genetic Variability Analysis for Yield and Yield-Associated Traits of Finger Millet [Eleusine coracana (L.) Gaertn.] Accessions at Mechara, Eastern Ethiopia
Authors: Chimdi Ababa , Tesso Bulti , Daba Chemeda
Published: June 2024

The genetic enhancement of any crop, including finger millet, requires a certain degree of genetic variation for effective utilization in crop improvement programs. However, in Ethiopia, there is limited information on the extent and pattern of genetic variability of finger millet collections under diversified agro-climatic conditions. This makes it difficult for a population to adapt to changing environmental conditions. As a result, the population may be more vulnerable to extinction, exposure to new diseases, low productivity, and selection acting on any genes that may provide disease resistance. Therefore, knowledge of genetic variability is crucial for breeders in order to develop new cultivars with desired traits that are beneficial for both farmers and breeders. The present investigation was carried out to estimating the genetic variability, heritability, and genetic advance in sixty four finger millet accessions for yield and yield-related traits at Mechara agricultural research center. The experimental design was laid out in 8 x 8 simple lattice design. The analysis of variance for mean sum of squares due to genotypes revealed highly significant differences for all the 17 quantitative characters. The genotypes showed the highest mean performance for grain yield ranged from 1.38 ton per hectare for ACC#208448 to 4.35 ton per hectare for ACC#230255. Whereas, genotypic and phenotypic coefficients of variation were found high for biomass yield, harvest index, and grain yield. Broad-sense heritability ranged from 50.12% for the number of fingers per ear to 93.18% for days to heading. High heritability coupled with high genetic advance as percent of mean were observed for leaf number, finger length, ear weight, thousand grain weight, biomass yield, and harvest index. In general, the results demonstrated that the finger millet accessions exhibited a high degree of genetic variability for the traits studied, which can be helpful for genetic enhancement.
Clinical Evaluation of Vital Inflamed Pulp Therapy as Treatment Options for Deciduous Molars with Periapical Periodontitis
Authors: Liu Yun , Sun Jin
Published: June 2024

Deciduous teeth, owing to their special anatomical structure, are highly vulnerable to apical periodontitis, particularly when afflicted with deep caries or other endodontic diseases. Traditionally, root canal therapy (RCT) has been the gold standard among pediatric dentists all along. Nevertheless, due to its inherent limitations, researchers are actively exploring alternative therapeutic approaches these years. This study introduces a new treatment modality known as vital inflamed pulp therapy (VIPT). The primary aim of this investigation was to assess the practical feasibility and therapeutic efficacy of VIPT in managing apical periodontitis in deciduous teeth comparing to RCT. After rigorous patients evaluation and examination, VIPT was chosen as the preferred treatment plan, followed by one year long-term follow-up observations. The outcomes revealed that most positive seamless healing of the periapical lesion without any complications in VIPT as the ones in RCT. Notably, the development of the permanent tooth embryo remained unaffected and progressed as anticipated. In conclusion, this study validates the therapeutic potential of VIPT in treating apical periodontitis in deciduous teeth, offering a novel approach for managing deciduous tooth diseases. The clinical application of this method is expected to enhance patient experiences and prognostic outcomes in the treatment of deciduous tooth pathologies.
On the Sublation, Inheritance and Innovation of Chinese Traditional Culture
Authors: Xiaoliang Ding
Published: June 2024

The transformation and development of contemporary Chinese society needs the third ideological enlightenment. The theme of this ideological enlightenment should be to lay the ideological foundation for "starting a new journey of building a socialist modern country in an all-round way", for "realizing the 14th Five-Year Plan and the long-term goal of 2035"; and comprehensive re-understanding of China's national character insufficiency; re-educate all citizens; sublate, inherit and innovate traditional culture; set up " Democracy and legal system", "Marketing Economy System". This paper specifically analyzes what content of traditional culture needs to be sublated, inherited and innovated. Discard the dross of national character, such as servility, deceive oneself and others, lack of seriousness in life and affairs, disrespect for contracts, disrespect for rules, like to ask for petty advantage, two-faced people, spectator mentality, pay attention to the collective but despise individual, do not pay attention to commerce, equalitarianism, good face, waste, etc.; inherit the excellent traditions of ancestors, such as "Virtue" and gentleman personality, benevolence, harmonious development between man and nature, unity of knowledge and practice, faithfulness and trustworthiness, pursuit of freedom, and studious, etc.; and more importantly, absorb the common excellent heritage of human civilization, reconstruct and innovate the national character in combination with China's reality, such as the spirit of respect for contracts and law, scientific spirit, creativity, innovation and entrepreneurship, the courage to express rationally, value and respect commerce, saving consciousness, and sense of cooperation, etc.
A Mathematical Model to Investigate How Vaccination Affect the Reproduction Number for COVID-19
Authors: Geofrey Nandwa Khayo , Harun Makwata , Samuel Muthiga , Edward Njuguna
Published: June 2024

In this paper a mathematical model that investigates how vaccination affects the dynamics of COVID-19 was considered. More particularly the model takes into account the waning rate of immunity after vaccination as well as administration of booster vaccine. Posititivity and boundedness of solutions of the model were proved. The disease free equilibrium of the model was determined and by using the next generation matrix method both the basic and effective reproduction numbers of the model were determined. Further, from the effective reproduction number, the minimum critical value of individuals to be vaccinated for containment of the diseases was determined. It was found that the value is less for a perfect vaccine compared to an imperfect vaccine. Numerical simulation of the model was done to determine how the parameters of interest in the study (waning rate of immunity, vaccination rate, administration of booster vaccine and efficacy of the vaccine) affect the effective reproduction number. The results show that increasing the rates of vaccination, administering booster vaccine will decrease the effective reproduction number while an increase in waning rate of immunity increases the effective reproduction number. The disease persist in the population due to the declining of immunity after vaccination which increases the effective reproduction number.
Research and Application of Temporary Plugging Agent Technology for High-Pressure Wells in Gas Storage Facilities
Authors: Dong Yang , Yi Zhang , Yongqiang Ren , Wei Zhang , Zhihong Wei , Shuhe Wu
Published: June 2024

Before various construction of gas storage well, in order to ensure safety, the reservoir must be temporarily blocked, degassed by circulation and no gas in the well can be operated. There are many temporary plugging methods for oil and gas wells. This paper studies the more advanced micro mesh gel temporary plugging technology in the industry and its application in gas storage wells, which mainly solves the problems of poor degradation backflow effect and difficult permeability recovery caused by weak formation stability after temporary plugging of gas storage wells. Research points: (1) On the basis of ordinary gel, through the fusion with cross-linking agent with special structure, a micron network polymer with significant steric hindrance effect on water molecules is finally formed, which improves the strength and stability of the gel system. (2) Add hexamethylene tetramine, bisphenol propane, delayed cross-linking agent and stabilizer into the polymer aqueous solution. It is verified by laboratory tests that the micron mesh gel can be formed after constant temperature coagulation for 3h~70h at ambient temperature of 90°C~170°C. (3) The temperature resistance, permeability and gel breaking of micron mesh gel are tested in the laboratory, and the results can meet the construction requirements. (4) After the temporary plugging operation of Well Su 49K-2X in the gas storage reservoir of Huabei Oilfield is completed, the degradation products of gel breaking will flow back smoothly to ensure that the well can be put into normal injection and production quickly. The test results show that: (1) Micron mesh gel has the advantages of long plugging validity, high temperature resistance, low viscosity after gel breaking, no residue, thorough gel breaking, no formation plugging pollution, and no impact on the original reservoir permeability. It is a new green plugging technology suitable for deep buried high-temperature depleted oil and gas reservoirs. (2) Before construction, the well temperature shall be measured to conduct test injection, so as to prevent gas invasion from affecting the construction effect. The pump injection displacement is designed to be redundant, so as to prevent the low molecular solution from not reaching the design position in the well on time, and the formation of solid rubber plugs at other positions in the well from affecting the construction.
Analysis of the flow field characteristics of a centrifugal blood pump in a heartbeat cycle
Authors: Zhang Jiahui , Yang Fan , Shao Chunlei
Published: June 2024

Blood pumps are medical devices that can temporarily or permanently replace the heart to perform the job of blood pumping. To study the internal flow field characteristics of the centrifugal blood pump, high-speed photography (HSP) images of the internal flow in the centrifugal blood pump were taken, and the internal flow field was numerically simulated. According to the heartbeat rule, the visualization experiment method and numerical simulation method for the internal flow of the centrifugal blood pump were developed. The internal flow rule of the centrifugal blood pump during a heartbeat cycle was revealed, as well as the influence of pump construction, flow rate, and other factors on the pump’s flow field. The results indicate that the flow rate and the rotor/stator interference of the blade and volute tongue cause two periodic changes in the pump pressure and velocity. One is the cycle of the heartbeat, and the other is the cycle of the blade’s rotation. The research results are of great significance in reducing the mechanical damage of blood, optimizing the structure of the pump and improving the stability of the centrifugal blood pump.
A survey on UK researchers’ views regarding their experiences with the de-identification, anonymisation, release methods and re-identification risk estimation for clinical trial datasets
Authors: Rodriguez Aryelly , Lewis Steff C , Eldridge Sandra , Jackson Tracy , Weir Christopher J
Published: June 2024

Background:

There are increasing pressures for anonymised datasets from clinical trials to be shared across the scientific community. However, there is no standardised set of recommendations on how to anonymise and prepare clinical trial datasets for sharing, while an ever-increasing number of anonymised datasets are becoming available for secondary research. Our aim was to explore the current views and experiences of researchers in the United Kingdom about de-identification, anonymisation, release methods and re-identification risk estimation for clinical trial datasets.

Methods:

We used an online exploratory cross-sectional descriptive survey that consisted of both open-ended and closed questions.

Results:

We had 38 responses to invitation from June 2022 to October 2022. However, 35 participants (92%) used internal documentation and published guidance to de-identify/anonymise clinical trial datasets. De-identification, followed by anonymisation and then fulfilling data holders’ requirements before access was granted (controlled access), was the most common process for releasing the datasets as reported by 18 (47%) participants. However, 11 participants (29%) had previous knowledge of re-identification risk estimation, but they did not use any of the methodologies. Experiences in the process of de-identifying/anonymising the datasets and maintaining such datasets were mostly negative, and the main reported issues were lack of resources, guidance, and training.

Conclusion:

The majority of responders reported using documented processes for de-identification and anonymisation. However, our survey results clearly indicate that there are still gaps in the areas of guidance, resources and training to fulfil sharing requests of de-identified/anonymised datasets, and that re-identification risk estimation is an underdeveloped area.
Developmental associations between cognition and adaptive behavior in intellectual and developmental disability
Authors: Dakopolos Andrew , Condy Emma , Smith Elizabeth , Harvey Danielle , Kaat Aaron J. , Coleman Jeanine , Riley Karen , Berry-Kravis Elizabeth , Hessl David
Published: June 2024

Background

Intellectual and developmental disabilities (IDDs) are associated with both cognitive challenges and difficulties in conceptual, social, and practical areas of living, commonly referred to as adaptive behavior (DSM–5). Although cross-sectional associations between intelligence or cognition and adaptive behavior have been reported in IDD populations, no study to date has examined whether developmental changes in cognition contribute to or track with changes in adaptive behavior. The present study sought to examine associations of longitudinal developmental change in domains of cognition (NIH Toolbox Cognition Battery, NIHTB-CB) and adaptive behavior domains (Vineland Adaptive Behavior Scales-3; VABS-3) including Socialization, Communication, and Daily Living Skills (DLS) over a two year period in a large sample of children, adolescents and young adults with IDD.

Methods

Three groups were recruited, including those with fragile X syndrome, Down syndrome, and other/idiopathic intellectual disability. Eligible participants (n = 263) included those who were between 6 and 26 years (mage = 15.52, sd = 5.17) at Visit 1, and who had a diagnosis of, or suspected intellectual disability (ID), including borderline ID, with a mental age of at least 3.0 years. Participants were given cognitive and adaptive behavior assessments at two time points over a two year period (m = 2.45 years, range = 1.27 to 5.56 years). In order to examine the association of developmental change between cognitive and adaptive behavior domains, bivariate latent change score (BLCS) models were fit to compare change in the three cognitive domains measured by the NIHTB-CB (Fluid Cognition, Crystallized Cognition, Total Cognition) and the three adaptive behavior domains measured by the VABS-3 (Communication, DLS, and Socialization).

Results

Over a two year period, change in cognition (both Crystallized and Total Composites) was significantly and positively associated with change in daily living skills. Also, baseline cognition level predicted growth in adaptive behavior, however baseline adaptive behavior did not predict growth in cognition in any model.

Conclusions

The present study demonstrated that developmental changes in cognition and adaptive behavior are associated in children and young adults with IDD, indicating the potential for cross-domain effects of intervention. Notably, improvements in DLS emerged as a primary area of adaptive behavior that positively related to improvements in cognition. This work provides evidence for the clinical, “real life” meaningfulness of changes in cognition detected by the NIHTB-CB in IDD, and provides empirical support for the NIHTB-CB as a fit-for-purpose performance-based outcome measure for this population.
iGenSig-Rx: an integral genomic signature based white-box tool for modeling cancer therapeutic responses using multi-omics data
Authors: Lee Sanghoon , Sun Min , Hu Yiheng , Wang Yue , Islam Md N. , Goerlitz David , Lucas Peter C. , Lee Adrian V. , Swain Sandra M. , Tang Gong , Wang Xiao-Song
Published: June 2024

Multi-omics sequencing is poised to revolutionize clinical care in the coming decade. However, there is a lack of effective and interpretable genome-wide modeling methods for the rational selection of patients for personalized interventions. To address this, we present iGenSig-Rx, an integral genomic signature-based approach, as a transparent tool for modeling therapeutic response using clinical trial datasets. This method adeptly addresses challenges related to cross-dataset modeling by capitalizing on high-dimensional redundant genomic features, analogous to reinforcing building pillars with redundant steel rods. Moreover, it integrates adaptive penalization of feature redundancy on a per-sample basis to prevent score flattening and mitigate overfitting. We then developed a purpose-built R package to implement this method for modeling clinical trial datasets. When applied to genomic datasets for HER2 targeted therapies, iGenSig-Rx model demonstrates consistent and reliable predictive power across four independent clinical trials. More importantly, the iGenSig-Rx model offers the level of transparency much needed for clinical application, allowing for clear explanations as to how the predictions are produced, how the features contribute to the prediction, and what are the key underlying pathways. We anticipate that iGenSig-Rx, as an interpretable class of multi-omics modeling methods, will find broad applications in big-data based precision oncology. The R package is available: https://github.com/wangxlab/iGenSig-Rx.
A single cell RNA sequence atlas of the early Drosophila larval eye
Authors: Raja Komal Kumar Bollepogu , Yeung Kelvin , Li Yumei , Chen Rui , Mardon Graeme
Published: June 2024

The Drosophila eye has been an important model to understand principles of differentiation, proliferation, apoptosis and tissue morphogenesis. However, a single cell RNA sequence resource that captures gene expression dynamics from the initiation of differentiation to the specification of different cell types in the larval eye disc is lacking. Here, we report transcriptomic data from 13,000 cells that cover six developmental stages of the larval eye. Our data show cell clusters that correspond to all major cell types present in the eye disc ranging from the initiation of the morphogenetic furrow to the differentiation of each photoreceptor cell type as well as early cone cells. We identify dozens of cell type-specific genes whose function in different aspects of eye development have not been reported. These single cell data will greatly aid research groups studying different aspects of early eye development and will facilitate a deeper understanding of the larval eye as a model system.
Genetic characteristics of chromosomally integrated carbapenemase gene (blaNDM−1) in isolates of Proteus mirabilis
Authors: Wang Qingyu , Dong Kai , Liu Xudong , Li Wanxiang , Bian Qianyu
Published: June 2024

Objective

This study aims to conduct an in-depth genomic analysis of a carbapenem-resistant Proteus mirabilis strain to uncover the distribution and mechanisms of its resistance genes.

Methods

The research primarily utilized whole-genome sequencing to analyze the genome of the Proteus mirabilis strain. Additionally, antibiotic susceptibility tests were conducted to evaluate the strain’s sensitivity to various antibiotics, and related case information was collected to analyze the clinical distribution characteristics of the resistant strain.

Results

Study on bacterial strain WF3430 from a tetanus and pneumonia patient reveals resistance to multiple antibiotics due to extensive use. Whole-genome sequencing exposes a 4,045,480 bp chromosome carrying 29 antibiotic resistance genes. Two multidrug-resistant (MDR) gene regions, resembling Tn6577 and Tn6589, were identified (MDR Region 1: 64.83 Kb, MDR Region 2: 85.64 Kbp). These regions, consist of integrative and conjugative elements (ICE) structures, highlight the intricate multidrug resistance in clinical settings.

Conclusion

This study found that a CR-PMI strain exhibits a unique mechanism for acquiring antimicrobial resistance genes, such as blaNDM−1, located on the chromosome instead of plasmids. According to the results, there is increasing complexity in the mechanisms of horizontal transmission of resistance, necessitating a comprehensive understanding and implementation of targeted control measures in both hospital and community settings.
Comparison of intravascular lithotripsy versus rotational atherectomy for the treatment of severe coronary artery calcification
Authors: Zhao Yuhao , Wang Ping , Zheng Ze , Shi Yuchen , Liu Jinghua
Published: June 2024

Background

Calcified lesions are one of the most challenging cases for PCI, where optimal angiographic results and satisfying outcomes are hard to achieve.

Methods

We evaluated the baseline clinical, procedures characteristics and outcomes of patients with severe coronary artery calcification (CAC) who underwent coronary intravascular lithotripsy (IVL) and rotational atherectomy (RA).

Results

Respectively 152 and 238 patients who underwent IVL and RA are enrolled from January 2023 to November 2023. Regarding demographic characteristics, the gender proportion, medical history of PCI and smoke history among groups reach statistical significance. Left anterior descending and right coronary artery were the main vessels treated in both groups. The 2.5 and 3.0 mm IVL balloons and 1.5 mm burr were the most commonly used. 99.3% cases were successfully implanted drug-eluting stents after IVL balloon pre-treatment, which was higher than in the group treated with RA. During hospitalization, there were no serious adverse events in the IVL group, but there were two adverse events in the RA group. Procedural complications were higher in the RA group than the IVL group (5.5% vs. 0.7%, P = 0.027).

Conclusions

IVL appears to be safe and effective for the treatment of severe CAC lesions compared to RA.
Autophagy-related molecular clusters identified as indicators for distinguishing active and latent TB infection in pediatric patients
Authors: Yu Yang , Hua Jie , Chen Liang
Published: June 2024

Background

Autophagy is crucial for controlling the manifestation of tuberculosis. This study intends to discover autophagy-related molecular clusters as biomarkers for discriminating between latent tuberculosis (LTBI) and active tuberculosis (ATB) in children through gene expression profile analysis.

Methods

The expression of autophagy modulators was examined in pediatric patients with LTBI and ATB utilizing public datasets from the Gene Expression Omnibus (GEO) collection (GSE39939 and GSE39940).

Results

In a training dataset (GSE39939), patients with LTBI and ATB exhibited the expression of autophagy-related genes connected with their active immune responses. Two molecular clusters associated with autophagy were identified. Compared to Cluster 1, Cluster 2 was distinguished through decreased adaptive cellular immune response and enhanced inflammatory activation, according to single-sample gene set enrichment analysis (ssGSEA). Per the study of gene set variation, Cluster 2’s differentially expressed genes (DEGs) played a role in synthesizing transfer RNA, DNA repair and recombination, and primary immunodeficiency. The peak variation efficiency, root mean square error, and area under the curve (AUC) (AUC = 0.950) were all lowered in random forest models. Finally, a seven-gene-dependent random forest profile was created utilizing the CD247, MAN1C1, FAM84B, HSZFP36, SLC16A10, DTX3, and SIRT4 genes, which performed well against the validation dataset GSE139940 (AUC = 0.888). The nomogram calibration and decision curves performed well in identifying ATB from LTBI.

Conclusions

In summary, according to the present investigation, autophagy and the immunopathology of TB might be correlated. Furthermore, this investigation established a compelling prediction expression profile for measuring autophagy subtype development risks, which might be employed as possible biomarkers in children to differentiate ATB from LTBI.
Thriving from work questionnaire: German translation and validation
Authors: Neidlinger Stephanie M. , Peters Susan E. , Gundersen Daniel A. , Felfe Jörg
Published: June 2024

Background

The Thriving from Work questionnaire is a comprehensive indicator of positive well-being for employees, applicable in both research and practical contexts. Current discussions underline the crucial impact that employment should have in enriching workers’ lives positively and meaningfully, along with the necessity for accurate and dependable tools to assess employee well-being. This study investigated the reliability, validity, and dimensionality of the translated German adaptation of the Thriving from Work questionnaire developed by Peters and colleagues [1, 2]. The questionnaire assesses work-related well-being with 30 items clustered in six domains: emotional and psychological well-being, social well-being, work-life integration, physical and mental well-being, basic needs for thriving, and experiences of work.

Methods

This study aimed to convert the Thriving at Work Questionnaire from English into German. We assessed the psychometric characteristics of the German version of the questionnaire by using item response theory with a sample of 567 German employees and examined its criterion validity.

Results

We found that the long and short German Thriving from Work questionnaire versions are reliable with good construct validity. Criterion validity was demonstrated by relationships with important work and life outcomes, such as life satisfaction, trust in the organizations’ management, general well-being, work-related fatigue, and work stress.

Conclusions

The current study demonstrated that the German language version of the questionnaire is both a reliable and valid measure of employee well-being. We discuss recommendations for further adaptation and future research.
A novel model of ambulatory teaching of residents in general practice in China: a cross-sectional study
Authors: Liang Lingbo , Liu Xiangping , Zhang Lin , Su Qiaoli
Published: June 2024

Background

This study aims to determine the satisfaction and future training needs of general practice residents participating in a novel model of ambulatory teaching aligned with the specifications for standardized residency training in outpatient management issued by the Chinese Medical Doctor Association (CMDA).

Methods

A cross-sectional survey of the satisfaction and training needs was conducted among general practice residents at West China Hospital, Sichuan University. Patient characteristics and preceptors’ feedback on the residents’ performance were also analyzed.

Results

The study involved 109 residents (30.28% men) and 161 patients (34.78% men; age: 52.63 ± 15.87 years). Residents reported an overall satisfaction score of 4.28 ± 0.62 with the ambulatory teaching program. Notably, residents scored lower in the Subjective-Objective-Assessment-Plan (SOAP) evaluation when encountering patients with the greater the number of medical problems (P < 0.001). Residents encountering patients with a shorter duration of illness (< 3 months) achieved higher scores than those with longer illness durations (≥ 3 months,P = 0.044). Residency general practitioners (GPs) were most challenged by applying appropriate and effective patient referrals (43/109; 39.45%). GPs expressed a strong desire to learn how to make decisions when facing challenging patient situations (4.51 ± 0.63).

Conclusion

This study suggests selecting patients with multiple comorbidities for ambulatory teaching and enhancing training on practical problem-solving abilities for GPs. The findings provide insights for the development of future ambulatory teaching programs.