bluuebunny/crossref_metadata_2025 huggingface dataset:
Longitudinal Analysis of Urinary Cytokines and Biomarkers in COVID-19 Patients with Subclinical Acute Kidney Injury
Authors: Casas-Aparicio Gustavo , Alvarado-de la Barrera Claudia , Escamilla-Illescas David , León-Rodríguez Isabel , Del Río-Estrada Perla Mariana , González-Navarro Mauricio , Calderón-Dávila Natalia , Olmedo-Ocampo Rossana , Castillejos-López Manuel , Figueroa-Hernández Liliana , Peralta-Prado Amy B. , Luna-Villalobos Yara , Piten-Isidro Elvira , Fernández-Campos Paola , Juárez-Díaz Alejandro , Piekarska Karolina , Ávila-Ríos Santiago
Published: December 2022

In hospitalized COVID-19 patients, disease progression leading to acute kidney injury (AKI) may be driven by immune dysregulation. We explored the role of urinary cytokines and their relationship with kidney stress biomarkers in COVID-19 patients before and after the development of AKI. Of 51 patients, 54.9% developed AKI. The principal component analysis indicated that in subclinical AKI, epidermal growth factor (EGF) and interferon (IFN)-α were associated with a lower risk of AKI, while interleukin-12 (IL-12) and macrophage inflammatory protein (MIP)-1β were associated with a higher risk of AKI. After the manifestation of AKI, EGF and IFN-α remained associated with a lower risk of AKI, while IL-1 receptor (IL-1R), granulocyte-colony stimulating factor (G-CSF), interferon-gamma-inducible protein 10 (IP-10) and IL-5 were associated with a higher risk of AKI. EGF had an inverse correlation with kidney stress biomarkers. Subclinical AKI was characterized by a significant up-regulation of kidney stress biomarkers and proinflammatory cytokines. The lack of EGF regenerative effects and IFN-α antiviral activity seemed crucial for renal disease progression. AKI involved a proinflammatory urinary cytokine storm.
The Role of the Gut Microbiome in Pediatric Obesity and Bariatric Surgery
Authors: Akagbosu Cynthia Omoge , Nadler Evan Paul , Levy Shira , Hourigan Suchitra Kaveri
Published: December 2022

Obesity affects 42.4% of adults and 19.3% of children in the United States. Childhood obesity drives many comorbidities including hypertension, fatty liver disease, and type 2 diabetes mellitus. Prior research suggests that aberrant compositional development of the gut microbiome, with low-grade inflammation, precedes being overweight. Therefore, childhood may provide opportunities for interventions that shape the microbiome to mitigate obesity-related diseases. Children with obesity have gut microbiota compositional and functional differences, including increased proinflammatory bacterial taxa, compared to lean controls. Restoration of the gut microbiota to a healthy state may ameliorate conditions associated with obesity and help maintain a healthy weight. Pediatric bariatric (weight-loss) surgery is an effective treatment for childhood obesity; however, there is limited research into the role of the gut microbiome after weight-loss surgery in children. This review will discuss the magnitude of childhood obesity, the importance of the developing microbiome in establishing metabolic pathways, interventions such as bariatric surgery that may modulate the gut microbiome, and future directions for the potential development of microbiome-based therapeutics to treat obesity.
GOLM1 and FAM49B: Potential Biomarkers in HNSCC Based on Bioinformatics and Immunohistochemical Analysis
Authors: Xi Yue , Zhang Tiange , Sun Wei , Liang Ruobing , Ganesh Sridha , Chen Honglei
Published: December 2022

Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers worldwide. We aimed to identify potential genetic markers that could predict the prognosis of HNSCC. A total of 44 samples of GSE83519 from Gene Expression Omnibus (GEO) datasets and 546 samples of HNSCC from The Cancer Genome Atlas (TCGA) were adopted. The differently expressed genes (DEGs) of the samples were screened by GEO2R. We integrated the expression information of DEGs with clinical data from GES42743 using the weighted gene co-expression network analysis (WGCNA). A total of 17 hub genes were selected by the module membership (|MM| > 0.8), and the gene significance (|GS| > 0.3) was selected from the turquoise module. GOLM1 and FAM49B genes were chosen based on single-gene analysis results. Survival analysis showed that the higher expression of GOLM1 and FAM49B genes was correlated with a worse prognosis of HNSCC patients. Immunohistochemistry and multiplex immunofluorescence techniques verified that GOLM1 and FAM49B genes were highly expressed in HNSCC cells, and high expressions of GOLM1 were associated with the pathological grades of HNSCC. In conclusion, our study illustrated a new insight that GOLM1 and FAM49B genes might be used as potential biomarkers to determine the development of HNSCC, while GOLM1 and FAM49B have the possibility to be prognostic indicators for HNSCC.
Arabidopsis Cys2/His2 Zinc Finger Transcription Factor ZAT18 Modulates the Plant Growth-Defense Tradeoff
Authors: Li Weiwei , Zhang Min , Zhang Tingyu , Liu Yueyan , Liu Lijing
Published: December 2022

Plant defense responses under unfavorable conditions are often associated with reduced growth. However, the mechanisms underlying the growth-defense tradeoff remain to be fully elucidated, especially at the transcriptional level. Here, we revealed a Cys2/His2-type zinc finger transcription factor, namely, ZAT18, which played dual roles in plant immunity and growth by oppositely regulating the signaling of defense- and growth-related hormones. ZAT18 was first identified as a salicylic acid (SA)-inducible gene and was required for plant responses to SA in this study. In addition, we observed that ZAT18 enhanced the plant immunity with growth penalties that may have been achieved by activating SA signaling and repressing auxin signaling. Further transcriptome analysis of the zat18 mutant showed that the biological pathways of defense-related hormones, including SA, ethylene and abscisic acid, were repressed and that the biological pathways of auxin and cytokinin, which are growth-related hormones, were activated by abolishing the function of ZAT18. The ZAT18-mediated regulation of hormone signaling was further confirmed using qRT-PCR. Our results explored a mechanism by which plants handle defense and growth at the transcriptional level under stress conditions.
Biologically Active Peptides from Venoms: Applications in Antibiotic Resistance, Cancer, and Beyond
Authors: Ageitos Lucía , Torres Marcelo D. T. , de la Fuente-Nunez Cesar
Published: December 2022

Peptides are potential therapeutic alternatives against global diseases, such as antimicrobial-resistant infections and cancer. Venoms are a rich source of bioactive peptides that have evolved over time to act on specific targets of the prey. Peptides are one of the main components responsible for the biological activity and toxicity of venoms. South American organisms such as scorpions, snakes, and spiders are important producers of a myriad of peptides with different biological activities. In this review, we report the main venom-derived peptide families produced from South American organisms and their corresponding activities and biological targets.
Macrophage Migration Inhibitory Factor in Major Depressive Disorder: A Multilevel Pilot Study
Authors: Swoboda Caroline , Deloch Lena , von Zimmermann Claudia , Richter-Schmidinger Tanja , Lenz Bernd , Kornhuber Johannes , Mühle Christiane
Published: December 2022

Macrophage migration inhibitory factor (MIF) is a controversially discussed inflammatory marker in major depressive disorder (MDD). While some studies show an association of high MIF protein levels with depression, animal models have yielded conflicting results. Thus, it remains elusive as to whether MIF plays an anti- or pro-depressive role. Therefore, we aimed to examine the potential of MIF at the genetic, expression and protein levels as a risk factor and biomarker to diagnose, monitor, or predict the course of MDD. Patients with a current major depressive episode (n = 66 with, and n = 63 without, prior medication) and remitted patients (n = 39) were compared with healthy controls (n = 61). Currently depressed patients provided a second blood sample after three weeks of therapy. Depression severity was assessed by self-evaluation and clinician rating scales. We genotyped for three MIF polymorphisms and analyzed peripheral MIF expression and serum levels. The absence of minor allele homozygous individuals in the large group of 96 female patients compared with 10–16% in female controls suggests a protective effect for MDD, which was not observed in the male group. There were no significant group differences of protein and expression levels, however, both showed predictive potential for the course of depression severity in some subgroups. While MIF protein levels, but not MIF expression, decreased during treatment, they were not associated with changes in depression severity. This project is the first to investigate three biological levels of MIF in depression. The data hint toward a genetic effect in women, but do not provide robust evidence for the utility of MIF as a biomarker for the diagnosis or monitoring of MDD. The observed predictive potential requires further analysis, emphasizing future attention to confounding factors such as sex and premedication.
Controllable Synthesis of Hybrid Dendrimers Composed of a Carbosilane Core and an Aromatic Shell: Does Size Matter?
Authors: Ardabevskaia Sofia N. , Chamkina Elena S. , Krasnova Irina Yu. , Milenin Sergey A. , Sukhova Ekaterina A. , Boldyrev Konstantin L. , Bakirov Artem V. , Serenko Olga A. , Shifrina Zinaida B. , Muzafarov Aziz M.
Published: December 2022

The controllable synthesis of novel hybrid dendrimers composed of flexible and rigid components was accomplished via effective Cu-catalyzed azide–alkyne cycloaddition (“click”) reaction between azide-functionalized carbosilane cores of two generations and monoethynyl-substituted hexaphenylbenzene dendron. A comprehensive analysis of the thermal and phase behavior of dendrimers allows us to detect a similar performance of dendrimers of both generations which, in our opinion, can be due to the similar ratio of rigid and flexible blocks in the dendrimers regardless the generation of carbosilane cores. The propensity to crystallization and ordering after the annealing procedure was confirmed by DSC and SWAXS. We found that hybrid dendrimers have a tendency to order depending on their constituents of different structures. This is in contrast to homogeneous dendrimers whose propensity to order is determined by the dendrimer molecule as a whole.
Novel p.G250A Mutation Associated with Chronic Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1)
Authors: Sándor Máté , Thiel Franziska G. , Schmid Margit , Demcsák Alexandra , Morales Granda Nataly C. , Németh Balázs Csaba , Vajda Sandor , Hoerning André , Sahin-Tóth Miklós
Published: December 2022

Inborn mutations in the digestive protease carboxypeptidase A1 (CPA1) gene may be associated with hereditary and idiopathic chronic pancreatitis (CP). Pathogenic mutations, such as p.N256K, cause intracellular retention and reduced secretion of CPA1, accompanied by endoplasmic reticulum (ER) stress, suggesting that mutation-induced misfolding underlies the phenotype. Here, we report the novel p.G250A CPA1 mutation found in a young patient with CP. Functional properties of the p.G250A mutation were identical to those of the p.N256K mutation, confirming its pathogenic nature. We noted that both mutations are in a catalytically important loop of CPA1 that is stabilized by the Cys248-Cys271 disulfide bond. Mutation of either or both Cys residues to Ala resulted in misfolding, as judged by the loss of CPA1 secretion and intracellular retention. We re-analyzed seven previously reported CPA1 mutations that affect this loop and found that all exhibited reduced secretion and caused ER stress of varying degrees. The magnitude of ER stress was proportional to the secretion defect. Replacing the naturally occurring mutations with Ala (e.g., p.V251A for p.V251M) restored secretion, with the notable exception of p.N256A. We conclude that the disulfide-stabilized loop of CPA1 is prone to mutation-induced misfolding, in most cases due to the disruptive nature of the newly introduced side chain. We propose that disease-causing CPA1 mutations exhibit abolished or markedly reduced secretion with pronounced ER stress, whereas CPA1 mutations with milder misfolding phenotypes may be associated with lower disease risk or may not be pathogenic at all.
Vasopressin as a Possible Link between Sleep-Disturbances and Memory Problems
Authors: Török Bibiána , Varga János , Zelena Dóra
Published: December 2022

Normal biological rhythms, including sleep, are very important for a healthy life and their disturbance may induce—among other issues—memory impairment, which is a key problem of many psychiatric pathologies. The major brain center of circadian regulation is the suprachiasmatic nucleus, and vasopressin (AVP), which is one of its main neurotransmitters, also plays a key role in memory formation. In this review paper, we aimed to summarize our knowledge on the vasopressinergic connection between sleep and memory with the help of the AVP-deficient Brattleboro rat strain. These animals have EEG disturbances with reduced sleep and impaired memory-boosting theta oscillation and show memory impairment in parallel. Based upon human and animal data measuring AVP levels, haplotypes, and the administration of AVP or its agonist or antagonist via different routes (subcutaneous, intraperitoneal, intracerebroventricular, or intranasal), V1a receptors (especially of hippocampal origin) were implicated in the sleep-memory interaction. All in all, the presented data confirm the possible connective role of AVP between biological rhythms and memory formation, thus, supporting the importance of AVP in several psychopathological conditions.
Oxidation-Cyclisation of Biphenyl Thioethers to Dibenzothiophenium Salts for Ultrarapid 18F-Labelling of PET Tracers
Authors: Sirindil Fatih , Maher Sinead , Schöll Michael , Sander Kerstin , Årstad Erik
Published: December 2022

18F-labelled radiotracers are in high demand and play an important role for diagnostic imaging with positron emission tomography (PET). Challenges associated with the synthesis of the labelling precursors and the incorporation of [18F]fluoride with practical activity yields at batch scale are the main limitations for the development of new 18F-PET tracers. Herein, we report a high-yielding and robust synthetic method to access naked dibenzothiophenium salt precursors of complex PET tracers and their labelling with [18F]fluoride. C-S cross-coupling of biphenyl-2-thioacetate with aryl halides followed by sequential oxidation-cyclisation of the corresponding thioethers gives dibenzothiophenium salts in good to excellent yields. Labelling of neutral and electron-deficient substrates with [18F]fluoride is ultrarapid and occurs under mild conditions (1 min at 90 °C) with high activity yields. The method enables facile synthesis of complex and sensitive radiotracers, as exemplified by radiofluorination of three clinically relevant PET tracers [18F]UCB-J, [18F]AldoView and [18F]FNDP, and can accelerate the development and clinical translation of new 18F-radiopharmaceuticals.
A Small RNA, UdsC, Interacts with the RpoHII mRNA and Affects the Motility and Stress Resistance of Rhodobacter sphaeroides
Authors: Spanka Daniel-Timon , Grützner Julian , Jäger Andreas , Klug Gabriele
Published: December 2022

sRNAs have an important role in the regulation of bacterial gene expression. The sRNA, UdsC, of Rhodobacter sphaeroides is derived from the 3′ UTR of the RSP_7527 mRNA, which encodes a hypothetical protein. Here, we showed the effect of UdsC on the resistance of Rhodobacter sphaeroides to hydrogen peroxide and on its motility. In vitro binding assays supported the direct interaction of UdsC with the 5′ UTR of the rpoHII mRNA. RpoHII is an alternative sigma factor with an important role in stress responses in R. sphaeroides, including its response to hydrogen peroxide. We also demonstrated that RpoHII controls the expression of the torF gene, which encodes an important regulator of motility genes. This strongly suggested that the observed effect of UdsC on TorF expression is indirect and mediated by RpoHII.
Influence of Single Deuterium Replacement on Frequency of Hydrogen Bond Dissociation in IFNA17 under the Highest Critical Energy Range
Authors: Basov Alexander , Drobotenko Mikhail , Svidlov Alexander , Bezhenar Maria , Gerasimenko Eugeny , Moiseev Arkadii , Malyshko Vadim , Dorohova Anna , Drozdov Andrey , Baryshev Mikhail , Dzhimak Stepan
Published: December 2022

The effect of single substitutions of protium for deuterium in hydrogen bonds between pairs of nitrogenous bases on the open states occurrence probability at high critical breaking energies of these bonds has been studied. The study was carried out using numerical methods based on the angular mathematical model of DNA. The IFNA17 gene was divided into three approximately equal parts. A comparison of the open states occurrence probability in these parts of the gene was done. To improve the accuracy of the results, a special data processing algorithm was developed. The developed methods have shown their suitability for taking into account the occurrence of open states in the entire range of high critical energies. It has been established that single 2H/1H substitutions in certain nitrogenous bases can be a mechanism for maintaining the vital activity of IFNA17 under critical conditions. In general, the developed method of the mathematical modeling provide unprecedented insight into the DNA behavior under the highest critical energy range, which greatly expands scientific understanding of nucleobases interaction.
Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends
Authors: Wei Yali , Lyu Ping , Bi Ruiye , Chen Xinyu , Yu Yanshen , Li Zucen , Fan Yi
Published: December 2022

Pulpal and periapical diseases are the most common dental diseases. The traditional treatment is root canal therapy, which achieves satisfactory therapeutic outcomes—especially for mature permanent teeth. Apexification, pulpotomy, and pulp revascularization are common techniques used for immature permanent teeth to accelerate the development of the root. However, there are obstacles to achieving functional pulp regeneration. Recently, two methods have been proposed based on tissue engineering: stem cell transplantation, and cell homing. One of the goals of functional pulp regeneration is to achieve innervation. Nerves play a vital role in dentin formation, nutrition, sensation, and defense in the pulp. Successful neural regeneration faces tough challenges in both animal studies and clinical trials. Investigation of the regeneration and repair of the nerves in the pulp has become a serious undertaking. In this review, we summarize the current understanding of the key stem cells, signaling molecules, and biomaterials that could promote neural regeneration as part of pulp regeneration. We also discuss the challenges in preclinical or clinical neural regeneration applications to guide deep research in the future.
Defining and Assessing Quality in IoT Environments: A Survey
Authors: Sgora Aggeliki , Chatzimisios Periklis
Published: December 2022

With the proliferation of multimedia services, Quality of Experience (QoE) has gained a lot of attention. QoE ties together the users’ needs and expectations to multimedia application and network performance. However, in various Internet of Things (IoT) applications such as healthcare, surveillance systems, traffic monitoring, etc., human feedback can be limited or infeasible. Moreover, for immersive augmented and virtual reality, as well as other mulsemedia applications, the evaluation in terms of quality cannot only focus on the sight and hearing senses. Therefore, the traditional QoE definition and approaches for evaluating multimedia services might not be suitable for the IoT paradigm, and more quality metrics are required in order to evaluate the quality in IoT. In this paper, we review existing quality definitions, quality influence factors (IFs) and assessment approaches for IoT. This paper also introduces challenges in the area of quality assessment for the IoT paradigm.
Variation in Prevertebral Soft Tissue Swelling after Staged Combined Multilevel Anterior–Posterior Complex Cervical Spine Surgery: Anterior Then Posterior (AP) versus Posterior Then Anterior–Posterior (PAP) Surgery
Authors: Eun Dong-Chan , Suguitan Anthony A. , Suk Kyung-Soo , Kim Hak-Sun , Kwon Ji-Won , Moon Seong-Hwan , Lee Yong-Ho , Lee Byung Ho
Published: December 2022

The influence of the sequence of surgery in the development of prevertebral soft tissue swelling (PSTS) in staged combined multilevel anterior–posterior complex spine surgery was examined. This study was conducted as a retrospective study of patients who underwent staged combined multilevel anterior–posterior complex cervical spine surgery from March 2014 to February 2021. Eighty-two patients were identified, of which fifty-seven were included in the final analysis after screening. PSTS was measured from routine serial monitoring lateral cervical radiographs prior to and after surgery for five consecutive days at each cervical level from C2 to C7 in patients who underwent anterior then posterior (AP) and posterior then anterior–posterior (PAP) surgery. The mean PSTS measurements significantly differed from the preoperative to postoperative monitoring days at all cervical levels (p = 0.0000) using repeated measures analysis of variance in both groups. PSTS was significantly greater in PAP than in AP at level C2 on postoperative day (POD) 1 (p = 0.0001). PSTS was more prominent at levels C2–4 during PODs 2–4 for both groups. In staged combined multilevel anterior–posterior complex spine surgery, PSTS is an inevitable complication. Therefore, surgeons should monitor PSTS after surgery when performing anterior–posterior complex cervical spine surgery, especially in the immediate postoperative period after PAP surgery.
Genomic Immune Evasion: Diagnostic and Therapeutic Opportunities in Head and Neck Squamous Cell Carcinomas
Authors: Kirtane Kedar , St. John Maie , Fuentes-Bayne Harry , Patel Sandip P. , Mardiros Armen , Xu Han , Ng Eric W. , Go William Y. , Wong Deborah J. , Sunwoo John B. , Welch John S.
Published: December 2022

Head and neck squamous cell cancers (HNSCCs) represent a diverse group of tumors emerging within different mucosal surfaces of the oral cavity, nasopharynx, oropharynx, larynx, and hypopharynx. HNSCCs share common clinical risk factors and genomic features, including smoking, alcohol, age, male sex, aneuploidy, and TP53 mutations. Viral initiating and contributing events are increasingly recognized in HNSCCs. While both Epstein–Barr Virus (EBV) and human papilloma virus (HPV) are observed, EBV is more frequently associated with nasopharyngeal cancers whereas HPV is associated with oropharyngeal cancers. HNSCCs are associated with high tumor mutational burden and loss of tumor suppressor gene function, especially in TP53 and X-linked genes. Multiple lines of evidence suggest that HNSCCs are subject to immunologic surveillance and immune-induced evolutionary pressure that correlate with negative clinical outcomes. This review will discuss genomic mechanisms related to immune-mediated pressures and propose prognostic and therapeutic implications of detectable immune escape mechanisms that drive tumorigenesis and disease progression.
Autologous Matrix-Induced Chondrogenesis (AMIC) for Focal Chondral Lesions of the Knee: A 2-Year Follow-Up of Clinical, Proprioceptive, and Isokinetic Evaluation
Authors: Bąkowski Paweł , Grzywacz Kamilla , Prusińska Agnieszka , Ciemniewska-Gorzela Kinga , Gille Justus , Piontek Tomasz
Published: December 2022

(1) Background: The autologous matrix-induced chondrogenesis (AMIC) is a bio-orthopedic treatment for articular cartilage damage. It combines microfracture surgery with the application of a collagen membrane. The aim of the present study was to report a medium-term follow-up of patients treated with AMIC for focal chondral lesions. (2) Methods: Fourty-eight patients treated surgically and 21 control participants were enrolled in the study. To evaluate the functional outcomes, the proprioceptive (postural stability, postural priority) and isokinetic (peak value of maximum knee extensor and flexor torque in relation to body mass and the total work) measurements were performed. To evaluate the clinical outcomes, the Lysholm score and the IKDC score were imposed. (3) Results: Compared to the preoperative values, there was significant improvement in the first 2 years after intervention in the functional as well as subjective outcome measures. (4) Conclusions: AMIC showed durable results in aligned knees.
The Revision and Application of Aurora in China: Based on Successful Intelligence
Authors: Cheng Li , Yan Jinglu , Ma Xiaochen , Chen Xiaoyu , Liu Zhengkui
Published: December 2022

Aurora Battery is a corresponding test of successful intelligence. This study aims to examine the factorial structure of the Chinese version of Aurora Battery and to investigate its internal consistency and validity, as well as to discover the developmental features of Chinese students. A total number of 2007 students were recruited from 13 schools across eastern, central, and western China, ranging from 4th to 8th grade (mean age = 12.29 years) and among them, 43.9% are girls. Confirmatory factor analysis (CFA) was used to examine the factorial structure. Among the tested models, a second-order factor model, in which the three ability factors serve as indicators of a general factor, provided an acceptable model fit to the data. Moreover, measurement invariance across gender and grades were supported, which suggests the mean scores of analytical, creative, and practical abilities are comparable in this research. The criterion-related validity analysis suggests that the battery and its three subscales have good criterion validity. The scale reliability analysis shows that the Cronbach’s alpha and the McDonald’s omega value of the whole test were .84 and .87, respectively, indicating the scale’s internal reliability is good. For ability differences among grades, students’ analytical and practical abilities increase across all grades, while creativity presents an upward trend from grade 4 to 6, followed by a downward trend from grade 6 to 7, and an increase from grade 7 to 8. Female students outperform male students on both analytical and creative ability, while with no obvious difference on practical abilities.
R&D Needs for the Design of the EU-DEMO HCPB ICD Balance of Plant in FP9
Authors: Perez-Martin Sara , Bubelis Evaldas , Hering Wolfgang , Barucca Luciana
Published: December 2022

During the Pre-Conceptual Design Phase of the EU-DEMO, two BOP solutions for WCLL and HCPB were elaborated, as close as possible to industrial standards. Nevertheless, each solution has open issues to be investigated, analytically and experimentally, in the Conceptual Design Phase (CDP). For the HCPB, the functionality and operability of the Helium-Molten Salt Heat Exchanger, and the coupling to a helium loop with a prototypic helium blower, is of primary interest. In addition, the operation of the pulse, dwell and transitions will be investigated within the new build infrastructure, HELOKA-US (Upgrade Storage), to be erected at KIT. The design requires a certain flexibility, since the final parameters of the Primary Heat Transfer System of DEMO may vary, due to plasma optimizations during CDP. HELOKA-US benefits from the high-pressure helium loop HELOKA-HP, erected to test HCPB-Breeding Blanket and First Wall modules, as well as from the competencies of preparing, handling and testing of various molten salts used for heat transfer optimization and natural convection.
Post-COVID-19 Psychiatric Symptoms in the Elderly: The Role of Gender and Resilience
Authors: Janiri Delfina , Tosato Matteo , Simonetti Alessio , Montanari Silvia , Terenzi Beatrice , Catinari Antonello , De Mori Lorenzo , Ferrajoli Gaspare Filippo , Kotzalidis Georgios D. , Landi Francesco , Bernabei Roberto , Sani Gabriele
Published: December 2022

COVID-19 represents an overwhelming stressor to mental health. Elderly individuals are particularly at risk, but it is still unclear whether the risk is equally distributed among men and women. The aim of this study was to define gender differences in persistent psychiatric symptoms after COVID-19 illness and to test their association with resilience factors. Methods: We assessed 348 individuals aged >65 years at a multidisciplinary post-COVID-19 service. Mood and anxiety symptoms were investigated, as well as psychological distress and resilience, as assessed with the Kessler-10 (K10) Scale and the Connor-Davidson Resilience Scale (CD-RISC), respectively. Multivariate and linear regression analyses were used to test the distribution patterns of psychiatric symptoms and resilience factors. Results: In the total sample, 214 (61.5%) were men (a mean age of 73.25 years ±6.04) and 134 (38.5%) were women (a mean age of 72.69 years ±6.43; p = 0.407). Men and women significantly differed in marital status (χ2 = 25.17; p < 0.001, more men were married), living alone (χ2 = 11.62; p < 0.01, fewer men were living alone), hospitalization during COVID-19 illness (χ2 = 12.35; p < 0.001, more men were hospitalized during COVID-19), and subjective health status before COVID-19 infection (χ2 = 4.32; p < 0.001, men reporting better subjective health than women). Women reported more psychiatric symptoms and fewer resilience factors than men. Low resilience levels significantly predicted psychological distress in both men and women. Conclusions: The female elderly population affected by COVID-19 showed a greater vulnerability to psychiatric symptoms. Our data point to the need to strengthen resilience resources, especially in women.
Identification of Circulating Exosomal microRNAs Associated with Radioiodine Refractory in Papillary Thyroid Carcinoma
Authors: Li Genpeng , Chen Wenjie , Gong Yanping , Wei Tao , Gong Rixiang , Zhu Jingqiang , Li Zhihui , Lei Jianyong
Published: December 2022

Papillary thyroid carcinoma (PTC) has a favorable prognosis, but a fraction of cases show progressive behaviors, becoming radioiodine refractory (RAIR) PTC. To explore circulating exosomal microRNAs (miRNAs) associated with RAIR PTC, the miRNA profiles in exosomes from parental and induced RAIR cell lines were firstly identified with a next-generation sequencing technique. The Na+/I− symporter (NIS) related miRNAs were then validated by quantitative real-time PCR (qRT-PCR) in plasma of PTC patients with non-131I-avid metastases and those with 131I-avid metastases. The regulation of exosomal miRNAs on NIS were also verified. We identified that miR-1296-5p, upregulation in exosomes from RAIR cell lines, and the plasma of patients with RAIR PTC achieved the largest areas under the curve (AUC) of 0.911 and that it is an independent risk factor for RAIR PTC. In addition, miR-1296-5p was abundantly detected in the tissue of RAIR PTC and can directly target downstream gene of NIS. Taken together, our findings suggested that circulating exosomal miRNAs, particularly miR-1296-5p, may be involved in the pathogenesis of RAIR PTC by directly targeting NIS.
A Longitudinal Analysis of the Internal Rotation and Shift (IRO/Shift) Test Following Arthroscopic Repair of Superior Rotator Cuff Lesions
Authors: Schwesig René , Fieseler George , Cornelius Jakob , Sendler Julia , Schulze Stephan , Hermassi Souhail , Delank Karl-Stefan , Laudner Kevin
Published: December 2022

Although the use of clinical tests to diagnose superior rotator cuff pathology is common, there is paucity in the research regarding the accuracy of such tests following arthroscopic repair. The aim of this study was to determine the accuracy of the IRO/Shift test compared to the Jobe test at 3 months and 6 months post-surgery for superior rotator cuff repair. Arthroscopic repair was conducted on 51 patients who were subsequently seen for clinical evaluation at 3 and 6 months following surgery. At 3 months post-surgery only 27% of the patients had a negative IRO/Shift test and 18% had a negative Jobe test. However, at 6 months 88% of the patients presented with a negative IRO/Shift test and 61% a negative Jobe test. When compared to each other, the IRO/Shift test and the Jobe test had 90% agreement pre-operatively, 71% agreement at 3 months post-surgery, and 67% agreement at 6 months. These results demonstrate that the accuracy of the IRO/Shift test and the Jobe test improved between 3 and 6 months following arthroscopic surgery of the superior rotator cuff, with the IRO/Shift test having better accuracy.
Suicide Risk in Post-COVID-19 Syndrome
Authors: Simonetti Alessio , Bernardi Evelina , Janiri Delfina , Mazza Marianna , Montanari Silvia , Catinari Antonello , Terenzi Beatrice , Tosato Matteo , Galluzzo Vincenzo , Ciciarello Francesca , Landi Francesco , Sani Gabriele
Published: December 2022

Post-acute sequelae of COVID-19 include several neuropsychiatric disorders. Little is known about the relationship between post-COVID-19 syndrome and suicidality. The aim of the study was to investigate the risk of suicide in subjects with persistent post-COVID-19 syndrome. One-thousand five-hundred eighty-eight subjects were assessed in the Post-Acute Care Service at the Fondazione Policlinico Universitario “Agostino Gemelli” IRCCS of Rome. Assessment included: (a) sociodemographic characteristics; (b) symptoms during and after COVID-19; (c) psychopathological evaluation. Participants were divided in those with (SUI) or without (NON SUI) suicide risk according to the Mini International Neuropsychiatric Interview. Additionally, subjects with SUI were split into those with high (HIGH SUI) and low (LOW SUI) suicide risk. Between-group comparisons were made with t-tests for continuous variables and χ2 tests for categorical variables. SUI showed greater percentages of physical complaints during and after COVID-19, greater percentages of psychiatric history and presence of psychiatric history in relatives, greater percentages of subjects previously undergoing psychopharmacotherapy, and greater levels of anxiety, mixed depressive symptoms, general psychopathology than NON SUI. HIGH SUI showed greater number of symptoms during and after COVID-19 and higher levels of mixed depressive symptoms than LOW SUI. Percentages of subjects undergoing psychotherapy was higher in LOW SUI than HIGH SUI. Greater levels of physical complaints and psychopathology during post-COVID-syndrome might enhance the risk of committing suicide. Treatment of physical complaints and psychotherapy might reduce suicide risk.
Physicians’ Knowledge, Attitude, and Experience of Pharmacogenomic Testing in China
Authors: Jia Tong , Wu Caiying , Hu Xiaowen , Li Sicong , Zhang Xinyi , Cai Yuchun , Chen Jing , Shi Luwen , Lu Christine Y. , Nie Xiaoyan
Published: December 2022

(1) Background: As prescribers, physicians play a decisive role in applying and promoting pharmacogenomic (PGx) testing in clinical practices. So far, little is known about physicians’ perspectives on PGx testing in China. The aim of this study was to assess physicians’ knowledge of, attitude towards, and experience of PGx testing in China. (2) Methods: A 39-question online survey was developed. Participants were physicians recruited through two platforms, MEDLINKER and “Dazhuanjia”. (3) Results: A total of 450 respondents completed the survey and 366 questionnaires were eligible for analysis based on the inclusion criteria. Among all included physicians, 275 (75.1%) had heard of PGx testing before. More than half rated their knowledge of PGx testing as “Fair” (61.5%) while 20.0% chose “Excellent” or “Good” and 18.6% chose “Poor” or “Terrible”. “Guidelines, consensus, and treatment paths for disease diagnosis and treatment” (72.7%) were the most preferred sources of information about PGx testing. Respondents were confident in their personal capacity to conduct PGx, with an average score of 3.30 ± 0.09 (out of 5.00). Most respondents (75.6%) believed that PGx could “help to improve efficacy and reduce the incidence of adverse reactions”. Targeted cancer therapy (score 78.95 ± 1.26 out of 100) was considered the field where PGx testing had its highest value. Lack of professionals and knowledge (n = 186, 67.6%), high costs of testing (n = 170, 61.8%), and lack of hospitals to offer PGx testing (n = 166, 60.4%) were identified as the primary obstacles to increasing the uptake of PGx testing in China. Academic conference (n = 213, 72.4%) was considered the most efficient way for physicians to obtain information about PGx testing. (4) Conclusions: Physicians in China have poor knowledge about PGx testing; nonetheless, they generally had confidence in their capacity to order PGx testing and positive attitudes towards the use of PGx testing in routine clinical practices. Future efforts to promote the uptake of PGx testing should focus on foundational education and practical training.
The Online HEARRING Counselling 1.0 Platform Provides Clinicians with Comprehensive Information on Hearing Device Solutions for Conductive, Mixed, and Sensorineural Hearing Loss
Authors: Hagen Rudolf , Rak Kristen , Kurz Anja , Baumgartner Wolf-Dieter , Gavilán Javier , van de Heyning Paul
Published: December 2022

A platform to help clinicians ensure that hearing device candidates are informed about the benefits and drawbacks of their recommended treatment option would be of clinical counselling benefit because it could help each candidate form realistic expectations about life with their treatment option. Following the World Café approach, 54 participants (surgeons, audiologist, and researchers) generated lists of the benefits and drawbacks of each treatment option for single-sided deafness (SSD) and bone conduction (BC) solutions. They then prioritized the benefits and drawbacks. After the World Café, literature research was performed on each topic to check if the statements (on benefits and drawbacks) are supported by quality peer-reviewed publications. Each participant was surveyed to ensure a collective agreement was reached. The HEARRING Counselling 1.0 Platform was developed. Thus far, sections for SSD and BC solutions have been completed. Initial feedback has been highly positive. The platform will be expanded to cover middle ear implant solutions and cochlear implants. A plan is in place to ensure the information continues to be timely. The HEARRING Counselling 1.0 helps clinicians provide comprehensive information to candidates about their treatment option and thereby helps establish that candidates have realistic expectations about the benefits and drawbacks of device use.
Human Services or Non-Human Services? How Online Retailers Make Service Decisions
Authors: Zhao Leilei , Wu Weiwei , Jiang Minghui
Published: December 2022

With the development of Internet technology, online shopping has become increasingly popular. Owing to the improvement of living standards, the quality of e-service has become one of the important criteria for online shopping, with online shopping consultation being one of the key services. At the same time, the emergence of new technologies such as Artificial Intelligent (AI) has allowed online retailers to increase the availability of non-human online shopping consultation services. Therefore, this paper investigates the service decision problem between human and non-human online shopping consultation services for online retailers in the online duopoly market. By constructing a Hotelling improvement model and applying it in a new way, considering consumer preferences for human services, this paper explores the impact of the optimal service level of human online shopping consultation services and consumers’ sensitivity to the service level of human services on online retailers’ pricing, service decisions, etc. Our research results show that consumers’ sensitivity to the service level of human online shopping consultation services has an impact on the demand and profit of online retailers. In addition, human online shopping consultation services are not always beneficial to the profitability. Furthermore, when two online retailers compete, the utility of the non-human online retailer’s service to consumers can influence the service decisions of the other online retailer.
Order-Picking Efficiency in E-Commerce Warehouses: A Literature Review
Authors: Li Yi , Zhang Ruining , Jiang Dandan
Published: December 2022

With the vigorous development of e-commerce, efficient order picking in e-commerce warehouses has attracted the attention of many scholars. To analyze the issues about order-picking efficiency currently being studied by relevant scholars in e-commerce warehouses, this paper reviews the literature on the application of order-picking strategy and efficiency optimization direction from 2020 to 2022. That mainly falls into two categories of picking systems: “picker-to-parts” and “parts-to-picker”. In the “picker-to-parts” picking system, more attention is paid to the picking strategies of storage assignment and order batching and the integration of multiple picking strategies. In contrast, in the “parts-to-picker” picking system, the main attention is on the man-machine cooperation in the Mobile Robot Fulfillment System (RMFS) and the Automated Storage and Retrieval System (AS/RS), as well as the coordination of the picking station. Further, this paper proposes future research directions for two categories of picking systems: further studying the order splitting strategy and order delivery issues; considering the dynamic uncertainties; combining the automated picking system with different picking strategies, and so on.
Spatial–Temporal Evolution of Ecosystem Service Value in Yunnan Based on Land Use
Authors: Wang Fuli , Fu Wei , Chen Jiancheng
Published: December 2022

Exploring the changes in ecosystem service value (ESV) caused by land use transition is important for regional ecological protection. According to the land use data from 2000 to 2020, the alterations in the land use and ESV in Yunnan over the past 20 years were calculated and analyzed. At the same time, spatial autocorrelation analysis was established to analyze the spatial relationships of ESV in 16 states and cities. The results show that from 2000 to 2020, cultivated land, grassland and unused land are on a decreasing trend, while forest land, water body and built-up land are on an increasing trend, with the largest change in built-up land. The total ESV is on an increasing trend, with water supply, gas regulation, climate regulation, environmental purification and hydrological regulation being the highest value of individual services. Spatially, the total ESV showed that high levels were in the northwest, southwest and east, and low levels were in the northeast, west and central parts. At the same time, it shows a positive spatial correlation with a weakening trend. It is dominated by high–high cluster and low–low cluster. The change in the value of ecological services in forest land, grassland and water body have a greater impact on the change in total service value in the region, and was the main contributing and sensitive factor.
Meta-Analysis of Effectiveness and Safety of Botulinum Toxin in the Treatment of Complex Regional Pain Syndrome
Authors: Su Yu-Chi , Hsieh Pei-Chun , Guo Yao-Hong , Lin Yu-Ching
Published: December 2022

Complex regional pain syndrome (CRPS) is characterized by pain, limited range of motion, swelling, skin changes, vasomotor instability, and patchy bone demineralization. Conservative management strategies for CRPS include physical and occupational therapy, psychosocial and behavioral therapy, and pharmacotherapy. However, some patients still experience CRPS symptoms after receiving conventional treatments. Therefore, botulinum toxin (BoNT) has been applied to patients with CRPS in several trials considering its analgesic effect in musculoskeletal and neuropathic pain; however, the results were controversial. We conducted the study to explore the effectiveness and safety of BoNT in patients with complex regional pain syndrome (CRPS). A search was performed using the following electronic databases up to 19 October 2022: PubMed, Embase, and Cochrane Library. We included both randomized controlled trials and nonrandomized controlled studies involving patients with complex regional pain syndrome managed with botulinum toxin. Cochrane risk-of-bias tool and Joanna Briggs Institute Critical Appraisal Checklist were used for quality assessment for randomized controlled trials and quasi-experimental studies. Only randomized controlled trials entered the meta-analysis. The primary outcome was the visual analogue scale of pain presented as a weighted mean difference (WMD) and 95% confidence interval (CI). The secondary outcome was the risk of adverse events presented as an odds ratio (OR) with 95% CI. We analyzed eight articles with 176 patients, including three randomized controlled trials with 62 participants. The age of the patients ranged from 23.8 to 51 years old. The duration of the disease ranged from 2.2 to 11.8 years. The proportion of females ranged from 16.6% to 100%. The route of administration of BoNT included: (1) lumbar sympathetic block (LSB), (2) intramuscular injection, (3) subcutaneous or intradermal injection (SC/ID). Improvement in pain was revealed in six studies, and adverse events were all self-limited and temporary. Meta-analysis revealed a significant reduction in pain at the first follow-up between 3 weeks to 1 month after intervention (WMD, −1.036, 95% CI, −1.673 to −0.400) but not at the second follow-up between 2 to 3 months after treatment (WMD, −0.895, 95% CI, −2.249 to 0.458). Subgroup analyses between LSB and SC/ID were nonsignificant at both follow-up periods (p = 0.422, 0.139). The risk of adverse events was similar between the BoNT and control group (OR, 0.698, 95% CI, 0.136 to 3.581). In conclusion, BoNT may be effective and safe for alleviating pain in patients with CRPS. However, we could not draw definite conclusions due to small sample size and high between-study heterogeneity. The limited number of participants may conceal the possibility of serious adverse events. Further large-scale randomized controlled trials are warranted to delineate the role of BoNT in CRPS.
(3-Methylene-2,3-dihydronaphtho[2,3-b][1,4]dioxin-2-yl)methanol
Authors: Suigo Lorenzo , Lodigiani Giulia , Straniero Valentina , Valoti Ermanno
Published: December 2022

(3-Methylene-2,3-dihydronaphtho[2,3-b][1,4]dioxin-2-yl)methanol was unexpectedly achieved as the main reaction product while applying a standard Johnson–Corey–Chaykovsky procedure to the 2,3-dihydronaphtho[2,3-b][1,4]dioxine-2-carbaldehyde, aiming at obtaining the corresponding epoxide. The structure of the recovered compound was confirmed through NMR and HRMS, the melting point was measured by DSC, and the organic purity was assessed using HPLC. We hypothesized the possible mechanism for the obtainment of this side product, which should involve the opening of the dioxane ring soon after the nucleophilic attack of the ylide to the carbonyl function. The consequent transfer of the negative charge allows the achievement of the phenolate function. The tautomer further rearranges, forming the unstable oxirane, which opening is favored by the acidic phenolic function, thus closing into the more stable six-membered ring compound. We confirmed the hypothesized reaction mechanism by applying the same reaction conditions while starting from the corresponding methyl ketone. This undesired compound, easily and quantitatively obtained by standard Johnson–Corey–Chaykovsky conditions, could pave the way to a new methodology for the obtainment of 2,3-disubstituted 1,4-naphthodioxanes, further derivatizable.