bluuebunny/crossref_metadata_2025 huggingface dataset:
Biological Characteristics of Poisonous Species of the Ranunculus L. Genus in the Flora of Nakhchivan
Authors: Safarova F.
Published: November 2023

Annual and perennial herbaceous plants belonging to the Ranunculus L. genus of the Ranunculaceae family were studied. It has been established that 103 out of 2000 species of the family are common in Azerbaijan and many of them are poisonous. In the taxonomic spectrum of the Nakhchivan flora, 55 species belonging to 14 genera of the Ranunculaceae family were identified. As a result of the research, toxic compounds were identified in 47 species belonging to 11 genera. Ranunculus L. is widespread in pastures and meadows and is represented by 33 species and 15 species, of which 13 are poisonous pasture plants. It is important to survey the distribution area of Ranunculus L. species, especially poisonous species, and improve the quality of pastures.
Chorological Analysis of Species of SedumL. Genus Distributed in the Lesser Caucasus
Authors: Akbarova Ch.
Published: November 2023

The Sedum L. genus is represented by 22 species in the Azerbaijan flora. According to the system of N. N. Portenier, 14 species distributed in the Lesser Caucasus were chorologically analyzed and their geographical elements were determined.
Taxonomic Analysis of Early Spring Plants of Shabran District Flora
Authors: Garakhani P. , Huseynova A. , Gasimova T. , Mirzeyeva Sh. , Aminli N. , Safgulieva T.
Published: November 2023

Studies the early spring flora of the Shabran district, provides a taxonomic analysis of wild grasses, populations of species belonging to the Ophrys and Orchis genera of the Orchidaceae family, the genus Tulipa of the Liliaceae family, the genera Muscariand Bellevalia of the Asparagaceae family, as well as the new range of the species Spiranthes spiralisfor the study area. The data is mapped based on GPS coordinates. As a result of our research in the Shabran district, 26 species belonging to 13 families and 23 genera were identified. Among the herbarium materials there are rare species listed in the Red Book of the Azerbaijan Republic (Crocus adami, Galanthus caucasica, Pyrus salicifolia, Rosa sosnovskyi, Tulipa biebersteiniana, Orchis oestrifera, Ophrys caucasica, etc.).
Abiotic Factors Impact on the Honey Bees Distribution in the Submontane Zone
Authors: Boymurodov Kh. , Rasulov M.
Published: November 2023

With the onset of the autumn season, a decrease in air temperature gradually weakens the vital activity of bees from the summer months. With a decrease in air temperature, the laying of eggs of Mother bees decreases and stops. The exchange of substances in bees slows down, they begin to prepare for cold air temperatures. As soon as the cold weather comes, they begin to fill the entire box holes with propolis. As a result of the studies carried out, it was also observed that Wasps grown at low temperatures have a short female, a short canoe, and in some cases the wing is completely absent. It has been studied that the temperature in bee hives rises above 35°С, negatively affects the development of worms, sometimes leading to their death.
Feed Crops of Nakhchivan Arid Mountain Lands
Authors: Mammadli T. , Bayramov B.
Published: November 2023

Presents information on feed crops of arid mountain lands of Nakhchivan. It is established that the most widespread group of feed grasses of the studied area includes Artemisia L., Salsola L., Kochia Roth, Atriplex L., Symphytum L., Astragalus L. and others. The distribution area of feed crops of these genera and mixed associations formed by them were studied.
Analysis of the Conflict Environment of Nursing Specialists
Authors: Abdyzhalieva A. , Mamatova K. , Chubakov T.
Published: November 2023

The article presents an analysis of conflict situations among nursing professionals. The analysis was carried out based on a questionnaire, which included various questions about the frequency of conflicts, the parties involved in them and their causes. In addition, this survey makes it possible to draw a conclusion about the psychological climate of the working environment based on an individual assessment of employees, considering conflict situations that arise in the team. The results of the survey are presented in the form of charts with quantitative indicators calculated while analyzing the responses of the data by employees. This scientific article allows you to study the nature of conflicts in institutions where nurses work, draw a conclusion about their opinion about the favorable working environment, and helps to identify the main causes of misunderstandings that periodically arise within the team.
Scientific and Practical Foundations of Teaching English in Rural Schools
Authors: Ergeshova G.
Published: November 2023

The problems of foreign language teaching in rural areas are analyzed and summarized in the article. The author points out that the rural school is not only an institution of general education, but also a source of the well-being of the country, of stability, of the basic development of the society and of the spirituality of the people. In the article the peculiarities of the social-psychological and personnel training of a teacher of a foreign language and his use of modern developments and information technologies in the teaching are considered.
Kabriyal ayağın mobilya tarihindeki yeri
Authors: MUTDOĞAN Alım Selin , ELİBOL Gülçin Cankız
Published: November 2023

Mobilya en yalın tanımıyla, kullanıcıların oturma, yatma, yemek yeme, depolama, çalışma ve benzeri eylemlerini rahat, verimli ve sağlıklı şekilde yerine getirebilmelerini sağlayan, çeşitli malzemelerden üretilmiş donatılardır. Mobilyanın belgeli tarihine bakıldığında, taş ve toprak bazlı sabit donatıların tarihinin MÖ 6500’lü yıllara kadar dayandığı görülmekle birlikte, günümüzdeki anlamıyla mobilyanın, kabaca 4 000 ila 4 500 yıllık bir geçmişinin olduğunu ifade etmek mümkündür. Bu çalışmada, klasik dönem mobilya tarihinin önemli bir öğesi olan Kabriyal ayağın tarihsel süreçte ortaya çıkışı, biçimsel ve anlamsal özellikleri ile birlikte araştırılmış; bu iki unsurda yaşanan değişiklikler analiz edilmiş ve Kabriyal ayağın, günümüze kadar uzanan etkisi aktarılmaya çalışılmıştır. Çalışma, nitel bir araştırmadır ve doküman taraması ve analizi üzerine kurulmuştur. Tarihsel süreci yansıtan dokümanlar, bilgi ve görsel belge şeklinde taranarak analiz edilmiş; yapılan analizler üzerinden mevcut durum tespiti yapılarak ve mobilya tasarımı tarihinin bu vazgeçilmez öğesine yönelik gelecek çıkarımları sunulmuştur. Çalışmanın, özellikle mobilya tarihi alanına yönelik literatüre katkı sağlaması ve tarihsel öğelerin biçim ve içeriklerinin bir arada ele alınması konusundaki farkındalığın artırılması yönünden, araştırmacılara ve tasarımcılara fayda sağlaması öngörülmektedir.
Vascular reconstruction of the decellularized biomatrix for whole-organ engineering—a critical perspective and future strategies
Authors: Gupta Santosh , Sharma Akriti , Petrovski Goran , Verma Rama Shanker
Published: November 2023

Whole-organ re-engineering is the most challenging goal yet to be achieved in tissue engineering and regenerative medicine. One essential factor in any transplantable and functional tissue engineering is fabricating a perfusable vascular network with macro- and micro-sized blood vessels. Whole-organ development has become more practical with the use of the decellularized organ biomatrix (DOB) as it provides a native biochemical and structural framework for a particular organ. However, reconstructing vasculature and re-endothelialization in the DOB is a highly challenging task and has not been achieved for constructing a clinically transplantable vascularized organ with an efficient perfusable capability. Here, we critically and articulately emphasized factors that have been studied for the vascular reconstruction in the DOB. Furthermore, we highlighted the factors used for vasculature development studies in general and their application in whole-organ vascular reconstruction. We also analyzed in detail the strategies explored so far for vascular reconstruction and angiogenesis in the DOB for functional and perfusable vasculature development. Finally, we discussed some of the crucial factors that have been largely ignored in the vascular reconstruction of the DOB and the future directions that should be addressed systematically.
An extended focused assessment with sonography in trauma ultrasound tissue-mimicking phantom for developing automated diagnostic technologies
Authors: Hernandez-Torres Sofia I. , Bedolla Carlos , Berard David , Snider Eric J.
Published: November 2023

Introduction: Medical imaging-based triage is critical for ensuring medical treatment is timely and prioritized. However, without proper image collection and interpretation, triage decisions can be hard to make. While automation approaches can enhance these triage applications, tissue phantoms must be developed to train and mature these novel technologies. Here, we have developed a tissue phantom modeling the ultrasound views imaged during the enhanced focused assessment with sonography in trauma exam (eFAST).

Methods: The tissue phantom utilized synthetic clear ballistic gel with carveouts in the abdomen and rib cage corresponding to the various eFAST scan points. Various approaches were taken to simulate proper physiology without injuries present or to mimic pneumothorax, hemothorax, or abdominal hemorrhage at multiple locations in the torso. Multiple ultrasound imaging systems were used to acquire ultrasound scans with or without injury present and were used to train deep learning image classification predictive models.

Results: Performance of the artificial intelligent (AI) models trained in this study achieved over 97% accuracy for each eFAST scan site. We used a previously trained AI model for pneumothorax which achieved 74% accuracy in blind predictions for images collected with the novel eFAST tissue phantom. Grad-CAM heat map overlays for the predictions identified that the AI models were tracking the area of interest for each scan point in the tissue phantom.

Discussion: Overall, the eFAST tissue phantom ultrasound scans resembled human images and were successful in training AI models. Tissue phantoms are critical first steps in troubleshooting and developing medical imaging automation technologies for this application that can accelerate the widespread use of ultrasound imaging for emergency triage.
Optomechanical assessment of photorefractive corneal cross-linking via optical coherence elastography
Authors: Frigelli Matteo , Büchler Philippe , Kling Sabine
Published: November 2023

Purpose: Corneal cross-linking (CXL) has recently been used with promising results to positively affect corneal refractive power in the treatment of hyperopia and mild myopia. However, understanding and predicting the optomechanical changes induced by this procedure are challenging.

Methods: We applied ambient pressure modulation based optical coherence elastography (OCE) to quantify the refractive and mechanical effects of patterned CXL and their relationship to energy delivered during the treatment on porcine corneas. Three different patterned treatments were performed, designed according to Zernike polynomial functions (circle, astigmatism, coma). In addition, three different irradiation protocols were analyzed: standard Dresden CXL (fluence of 5.4 J/cm2), accelerated CXL (fluence of 5.4 J/cm2), and high-fluence CXL (fluence of 16.2 J/cm2). The axial strain distribution in the stroma induced by ocular inflation (Δp = 30 mmHg) was quantified, maps of the anterior sagittal curvature were constructed and cylindrical refraction was assessed.

Results: Thirty minutes after CXL, there was a statistically significant increase in axial strain amplitude (p < 0.050) and a reduction in sagittal curvature (p < 0.050) in the regions treated with all irradiation patterns compared to the non-irradiated ones. Thirty-6 hours later, the non-irradiated regions showed compressive strains, while the axial strain in the CXL-treated regions was close to zero, and the reduction in sagittal curvature observed 30 minutes after the treatment was maintained. The Dresden CXL and accelerated CXL produced comparable amounts of stiffening and refractive changes (p = 0.856), while high-fluence CXL produced the strongest response in terms of axial strain (6.9‰ ± 1.9‰) and refractive correction (3.4 ± 0.9 D). Tripling the energy administered during CXL resulted in a 2.4-fold increase in the resulting refractive correction.

Conclusion: OCE showed that refractive changes and alterations in corneal biomechanics are directly related. A patient-specific selection of both, the administered UV fluence and the irradiation pattern during CXL is promising to allow customized photorefractive corrections in the future.
Exploring maternal-fetal interface with in vitro placental and trophoblastic models
Authors: Liu Xinlu , Wang Gang , Huang Haiqin , Lv Xin , Si Yanru , Bai Lixia , Wang Guohui , Li Qinghua , Yang Weiwei
Published: November 2023

The placenta, being a temporary organ, plays a crucial role in facilitating the exchange of nutrients and gases between the mother and the fetus during pregnancy. Any abnormalities in the development of this vital organ not only lead to various pregnancy-related disorders that can result in fetal injury or death, but also have long-term effects on maternal health. In vitro models have been employed to study the physiological features and molecular regulatory mechanisms of placental development, aiming to gain a detailed understanding of the pathogenesis of pregnancy-related diseases. Among these models, trophoblast stem cell culture and organoids show great promise. In this review, we provide a comprehensive overview of the current mature trophoblast stem cell models and emerging organoid models, while also discussing other models in a systematic manner. We believe that this knowledge will be valuable in guiding further exploration of the complex maternal-fetal interface.
A novel prognostic signature and immune microenvironment characteristics associated with disulfidptosis in papillary thyroid carcinoma based on single-cell RNA sequencing
Authors: Liao Zhenyu , Cheng Ye , Zhang Huiru , Jin Xing , Sun Hanxing , Wang Yue , Yan Jiqi
Published: November 2023

Background: Disulfidptosis is a newly discovered form of regulated cell death. The research on disulfidptosis and tumor progression remains unclear. Our research aims to explore the relationship between disulfidptosis-related genes (DRGs) and the clinical outcomes of papillary thyroid carcinoma (PTC), and its interaction on the tumor microenvironment.

Methods: The single-cell RNA seq data of PTC was collected from GEO dataset GSE191288. We illustrated the expression patterns of disulfidptosis-related genes in different cellular components in thyroid cancer. LASSO analyses were performed to construct a disulfidptosis associated risk model in TCGA-THCA database. GO and KEGG analyses were used for functional analyses. CIBERSORT and ESTIMATE algorithm helped with the immune infiltration estimation. qRT‒PCR and flow cytometry was performed to validate the hub gene expression and immune infiltration in clinical samples.

Results: We clustered PTC scRNA seq data into 8 annotated cell types. With further DRGs based scoring analyses, we found endothelial cells exhibited the most relationship with disulfidptosis. A 4-gene risk model was established based on the expression pattern of DRGs related endothelial cell subset. The risk model showed good independent prognostic value in both training and validation dataset. Functional enrichment and genomic feature analysis exhibited the significant correlation between tumor immune infiltration and the signature. The results of flow cytometry and immune infiltration estimation showed the higher risk scores was related to immuno-suppressive tumor microenvironment in PTC.

Conclusion: Our study exhibited the role of disulfidptosis based signature in the regulation of tumor immune microenvironment and the survival of PTC patients. A 4-gene prognostic signature (including SNAI1, STC1, PKHD1L1 and ANKRD37) was built on the basis of disulfidptosis related endothelial cells. The significance of clinical outcome and immune infiltration pattern was validated robustly.
Safety and effectiveness of tigecycline combination therapy in renal transplant patients with infection due to carbapenem-resistant gram-negative bacteria
Authors: Wang Qin , Liao Guiyi , Xia Quan , Ge Chaoliang , Ding Handong
Published: November 2023

Background

Carbapenem-resistant gram-negative bacterial (CRGNB) infections are increasing among kidney transplant recipients, and effective therapeutic options are limited. This study aimed to investigate the efficacy and adverse events associated with combination therapy tigecycline in renal transplant patients with CRGNB infections.

Methods

This study retrospectively analyzed 40 Chinese patients with confirmed or suspected CRGNB infections who received tigecycline therapy. The patients’ case features and clinical and microbiological data were analyzed.

Results

A total of 40 renal transplant recipients received tigecycline therapy for a median duration of 9 (range, 3–25) days. CRGNB isolates were obtained from the organ preservation solution of the donor kidney in 28 patients, with confirmed transmission in 4 patients. Infections were detected in the bloodstream, urinary tract, sputum, and wound. The most prevalent isolates were Klebsiella pneumoniae (75%, 30/40), Acinetobacter baumannii (15%, 6/40), and Escherichia coli (10%, 4/40). A clinical response was observed in 32 (80%) patients. The 28-day all-cause mortality rate was 7.5% (3/40), while the one-year all-cause mortality rate was 2.5% (1/40). While one patient died owing to severe pancreatitis, no serious adverse events related to tigecycline therapy were reported. However, multiple indices of liver function and pancreatitis precursors increased after treatment with tigecycline compared to before treatment.

Conclusion

Tigecycline therapy appears to be well tolerated in renal transplant recipients with multidrug-resistant bacterial infections. Nevertheless, attention should be paid to adverse reactions related to tigecycline therapy, especially gastrointestinal reactions, and the related laboratory tests should be closely monitored.
The Dlt and LiaFSR systems derepress SpeB production independently in the Δpde2 mutant of Streptococcus pyogenes
Authors: Faozia Sabrina , Hossain Tasmim , Cho Kyu Hong
Published: November 2023

The second messenger molecule, c-di-AMP, plays a critical role in pathogenesis and virulence in S. pyogenes. We previously reported that deleting the c-di-AMP phosphodiesterase gene pde2 severely suppresses SpeB production at the transcriptional level. We performed transposon mutagenesis to gain insight into the mechanism of how Pde2 is involved in SpeB regulation. We identified one of the genes of the dlt operon, dltX, as a suppressor of the SpeB-null phenotype of the Δpde2 mutant. The dlt operon consists of five genes, dltX, dltA, dltB, dltC, and dltD in many Gram-positive bacteria, and its function is to incorporate D-alanine into lipoteichoic acids. DltX, a small membrane protein, is a newly identified member of the operon. The in-frame deletion of dltX or insertional inactivation of dltA in the Δpde2 mutant restored SpeB production, indicating that D-alanylation is crucial for the suppressor phenotype. These mutations did not affect the growth in lab media but showed increased negative cell surface charge and enhanced sensitivity to polymyxin B. Considering that dlt mutations change cell surface charge and sensitivity to cationic antimicrobial peptides, we examined the LiaFSR system that senses and responds to cell envelope stress. The ΔliaR mutation in the Δpde2 mutant also derepressed SpeB production, like the ΔdltX mutation. LiaFSR controls speB expression by regulating the expression of the transcriptional regulator SpxA2. However, the Dlt system did not regulate spxA2 expression. The SpeB phenotype of the Δpde2ΔdltX mutant in higher salt media differed from that of the Δpde2ΔliaR mutant, suggesting a unique pathway for the Dlt system in SpeB production, possibly related to ion transport or turgor pressure regulation.
Operation time and clinical outcomes for open infrarenal abdominal aortic aneurysms to remain stable in the endovascular era
Authors: Gruber M. , Sotir A. , Klopf J. , Lakowitsch S. , Domenig C. , Wanhainen A. , Neumayer C. , Busch A. , Eilenberg W.
Published: November 2023

Objective

Endovascular aortic repair (EVAR) has become a routine procedure worldwide. Ultimately, the increasing number of EVAR cases entails changing conditions for open surgical repair (OSR) regarding patient selection, complexity, and surgical volume. This study aimed to assess the time trends of open abdominal aortic aneurysm (AAA) repair in a high-volume single center in Austria over a period of 20 years, focusing on the operation time and clinical outcomes.

Materials and methods

A retrospective analysis of all patients treated for infrarenal AAAs with OSR or EVAR between January 2000 and December 2019 was performed. Infrarenal AAA was defined as the presence of a >10-mm aortic neck. Cases with ruptured or juxtarenal AAAs were excluded from the analysis. Two cohorts of patients treated with OSR at different time periods, namely, 2000–2009 and 2010–2019, were assessed regarding demographical and procedure details and clinical outcomes. The time periods were defined based on the increasing single-center trend toward the EVAR approach from 2010 onward.

Results

A total of 743 OSR and 766 EVAR procedures were performed. Of OSR cases, 589 were infrarenal AAAs. Over time, the EVAR to OSR ratio was stable at around 50:50 (p = 0.488). After 2010, history of coronary arterial bypass (13.4% vs. 7.2%, p = 0.027), coronary artery disease (38.1% vs. 25.1%, p = 0.004), peripheral vascular disease (35.1% vs. 21.3%, p = 0.001), and smoking (61.6% vs. 34.3%, p < 0.001) decreased significantly. Age decreased from 68 to 66 years (p = 0.023). The operation time for OSR remained stable (215 vs. 225 min, first vs. second time period, respectively, p = 0.354). The intraoperative (5.8% vs. 7.2%, p = 0.502) and postoperative (18.3% vs. 20.8%, p = 0.479) complication rates also remained stable. The 30-day mortality rate did not change over both time periods (3.0% vs. 2.4%, p = 0.666).

Conclusion

Balanced EVAR to OSR ratio, similar complexity of cases, and volume over the two decades in OSR showed stable OSR time without compromise in clinical outcomes.
Climate change and human footprint increase insect defoliation across central boreal forests of Canada
Authors: Kalamandeen Michelle , Gulamhussein Imran , Castro Jose Bermudez , Sothe Camile , Rogers Cheryl A. , Snider James , Gonsamo Alemu
Published: November 2023

Anthropogenic climate change is contributing to increased insect infestation globally, leading to pest population growth, expansion of niche and geographic ranges as well as increased outbreak frequencies, resulting in economic losses and reduction in food security. In recent years, spongy moth (Lymantria dispar dispar), jack pine budworm (Choristoneura pinus pinus), large aspen tortrix (Choristoneura conflictana) and spruce budworm (Choristoneura fumiferana) caused widespread defoliation across one of Canada’s most forested provinces, Ontario. Observations of such outbreaks have been limited to field sightings around Ontario, with few studies focused on mapping of outbreak occurrence across the province or exploring potential anthropogenic and climatic drivers of infestation. Using random forest probability estimates and satellite data resampled to 1 km spatial resolution from the Moderate Resolution Imaging Spectroradiometer (MODIS), we reveal greater expansion of insect defoliation across Ontario between 2018 and 2020 than previously recorded. Much of the geographic expansion of outbreaks was driven by increasing temperature, and the proximity of roads. With ongoing global warming and growing economic development, infestations will not only continue to increase across Ontario but also expand northward due to their responses to accelerated warming at higher latitudes. This expansion presents an important and alarming new challenge for forest conservation and management in Ontario, in particular, and Canada in general.
Genetic characteristics of common variable immunodeficiency patients with autoimmunity
Authors: Liu Zhihui , Lu Chenyang , Qing Pingying , Cheng Ruijuan , Li Yujie , Guo Xue , Chen Ye , Ying Zhiye , Yu Haopeng , Liu Yi
Published: November 2023

Background: The pathogenesis of common variable immunodeficiency disorder (CVID) is complex, especially when combined with autoimmunity. Genetic factors may be potential explanations for this complex situation, and whole genome sequencing (WGS) provide the basis for this potential.

Methods: Genetic information of patients with CVID with autoimmunity, together with their first-degree relatives, was collected through WGS. The association between genetic factors and clinical phenotypes was studied using genetic analysis strategies such as sporadic and pedigree.

Results: We collected 42 blood samples for WGS (16 CVID patients and 26 first-degree relatives of healthy controls). Through pedigree, sporadic screening strategies and low-frequency deleterious screening of rare diseases, we obtained 9,148 mutation sites, including 8,171 single-nucleotide variants (SNVs) and 977 Insertion-deletions (InDels). Finally, we obtained a total of 28 candidate genes (32 loci), of which the most common mutant was LRBA. The most common autoimmunity in the 16 patients was systematic lupus erythematosis. Through KEGG pathway enrichment, we identified the top ten signaling pathways, including “primary immunodeficiency”, “JAK-STAT signaling pathway”, and “T-cell receptor signaling pathway”. We used PyMOL to predict and analyse the three-dimensional protein structures of the NFKB1, RAG1, TIRAP, NCF2, and MYB genes. In addition, we constructed a PPI network by combining candidate mutants with genes associated with CVID in the OMIM database via the STRING database.

Conclusion: The genetic background of CVID includes not only monogenic origins but also oligogenic effects. Our study showed that immunodeficiency and autoimmunity may overlap in genetic backgrounds.

Clinical Trial Registration: identifier ChiCTR2100044035
Whole mitochondrial genome analysis in highland Tibetans: further matrilineal genetic structure exploration
Authors: Li Xin , Zhang Xianpeng , Yu Ting , Ye Liping , Huang Ting , Chen Ying , Liu Shuhan , Wen Youfeng
Published: November 2023

Introduction: The Qinghai–Tibet Plateau is one of the last terrestrial environments conquered by modern humans. Tibetans are among the few high-altitude settlers in the world, and understanding the genetic profile of Tibetans plays a pivotal role in studies of anthropology, genetics, and archaeology.

Methods: In this study, we investigated the maternal genetic landscape of Tibetans based on the whole mitochondrial genome collected from 145 unrelated native Lhasa Tibetans. Molecular diversity indices, haplotype diversity (HD), Tajima’s D and Fu’s Fs were calculated and the Bayesian Skyline Plot was obtained to determining the genetic profile and population fluctuation of Lhasa Tibetans. To further explore the genetic structure of Lhasa Tibetans, we collected 107 East Asian reference populations to perform principal component analysis (PCA), multidimensional scaling (MDS), calculated Fst values and constructed phylogenetic tree.

Results: The maternal genetic landscape of Tibetans showed obvious East Asian characteristics, M9a (28.28%), R (11.03%), F1 (12.41%), D4 (9.66%), N (6.21%), and M62 (4.14%) were the dominant haplogroups. The results of PCA, MDS, Fst and phylogenetic tree were consistent: Lhasa Tibetans clustered with other highland Tibeto-Burman speakers, there was obvious genetic homogeneity of Tibetans in Xizang, and genetic similarity between Tibetans and northern Han people and geographically adjacent populations was found. In addition, specific maternal lineages of Tibetans also be determined in this study.

Discussion: In general, this study further shed light on long-time matrilineal continuity on the Tibetan Plateau and the genetic connection between Tibetans and millet famers in the Yellow River Basin, and further revealed that multiple waves of population interaction and admixture during different historical periods between lowland and highland populations shaped the maternal genetic profile of Tibetans.
Palmatine treats urticaria by reducing inflammation and increasing autophagy
Authors: Xiao Tian , Yu Xingzhi , Yang Liping , Duan Xiaohua
Published: November 2023

Introduction

Chronic spontaneous urticaria (CSU) is mainly manifested as wheals and erythema on the skin accompanied by itching, which will cause emotional anxiety and seriously affect the quality of life in patients. Palmatine (PAL) is a main chemical component of Yajieshaba, which has been found to effectively alleviate the symptoms of food allergy. However, its role and mechanism in CSU remain unclear. The present study aimed to investigate the protective effect of PAL on CSU rats.

Methods

We replicated the CSU rat model by intraperitoneal injection of ovalbumin (OVA) in rats on days 0, 2, 4, and 14, with a double dose given on the last challenge. PAL, loratadine and saline were given by gavage from day 5 to day 14. We observed the skin pathologic changes, mast cell degranulation, immune factor levels, inflammatory response and autophagy-related protein expression in CSU rats.

Results

We found PAL treatment to be effective in alleviating CSU-like skin lesions and reducing itching and mast cell degranulation in rats. Compared with the OVA group, the levels of immune and inflammatory factors were significantly reduced, neutrophil recruitment was alleviated, suggesting a reduced inflammatory response. The autophagy results showed that PAL further increased the expression of LC3, Beclin-1 and p-LKB1, p-AMPK, Atg5, Atg12 and Atg5-Atg12, while P62 and p-p70S6K1 expression decreased. They collectively suggested that autophagic flux was activated after PAL treatment. However, there was an increase in the expression of LC3I, probably due to the fact that PAL induced its accumulation in order to provide substrate for the generation of more LC3II.

Discussion

Overall, PAL had a protective effect on CSU in normal rats, activated the expression of autophagy and improved the inflammatory response.
Association of inflammatory indicators with intensive care unit mortality in critically ill patients with coronary heart disease
Authors: Cheng Yuan , Chen Yang , Mao Mengxia , Wang Ruixuan , Zhu Jun , He Qing
Published: November 2023

Objective

Coronary heart disease (CHD) is one of the major cardiovascular diseases, a common chronic disease in the elderly and a major cause of disability and death in the world. Currently, intensive care unit (ICU) patients have a high probability of concomitant coronary artery disease, and the mortality of this category of patients in the ICU is receiving increasing attention. Therefore, the aim of this study was to verify whether the composite inflammatory indicators are significantly associated with ICU mortality in ICU patients with CHD and to develop a simple personalized prediction model.

Method

7115 patients from the Multi-Parameter Intelligent Monitoring in Intensive Care Database IV were randomly assigned to the training cohort (n = 5692) and internal validation cohort (n = 1423), and 701 patients from the eICU Collaborative Research Database served as the external validation cohort. The association between various inflammatory indicators and ICU mortality was determined by multivariate Logistic regression analysis and Cox proportional hazards model. Subsequently, a novel predictive model for mortality in ICU patients with CHD was developed in the training cohort and performance was evaluated in the internal and external validation cohorts.

Results

Various inflammatory indicators were demonstrated to be significantly associated with ICU mortality, 30-day ICU mortality, and 90-day ICU mortality in ICU patients with CHD by Logistic regression analysis and Cox proportional hazards model. The area under the curve of the novel predictive model for ICU mortality in ICU patients with CHD was 0.885 for the internal validation cohort and 0.726 for the external validation cohort. The calibration curve showed that the predicted probabilities of the model matched the actual observed probabilities. Furthermore, the decision curve analysis showed that the novel prediction model had a high net clinical benefit.

Conclusion

In ICU patients with CHD, various inflammatory indicators were independent risk factors for ICU mortality. We constructed a novel predictive model of ICU mortality risk in ICU patients with CHD that had great potential to guide clinical decision-making.
Assessment of thermocline depth bias in the Seychelles-Chagos Thermocline Ridge of the Southwestern Indian Ocean simulated by the CMIP6 models
Authors: Mubarrok Saat , Azminuddin Fuad , Jang Chan Joo
Published: November 2023

The Seychelles-Chagos Thermocline Ridge (SCTR, 5°S-10°S, 50°E-80°E) is a unique open-ocean upwelling region in the southwestern Indian Ocean. Due to the negative wind stress curl between the equatorial westerlies and southeasterly trade winds, SCTR is known as a strong upwelling region with high biological productivity, providing a primary fishing zone for the surrounding countries. Given its importance in shaping the variability of the Indian Ocean climate by understanding the sea-air interaction and its dynamics, the simulation of SCTR is evaluated using outputs from the Coupled Model Intercomparison Project Phase Sixth (CMIP6). Compared to observations, 23 out of 27 CMIP6 models tend to simulate considerably deeper SCTR thermocline depth (defined as the 20°C isotherm depth (D20))– a common bias in climate models. The deep bias is related to the easterly wind bias in the equatorial to southern Indian Ocean, which is prominent in boreal summer and fall. This easterly wind bias produces a weak annual mean Ekman pumping, especially in the boreal fall. Throughout the year, the observed Ekman pumping is positive and is driven by two components: the curl term, is associated with the wind stress curl, leads to upwelling during boreal summer to fall; the beta term, is linked to planetary beta and zonal wind stress, contributes to downwelling during boreal spring to fall. However, the easterly wind bias in the CMIP6 increases both the positive curl and negative beta terms. The beta term bias offsets the curl term bias and reduces the upwelling velocity. Furthermore, the easterly wind bias is likely caused by the reduced east-west sea surface temperature (SST) difference associated with a pronounced warm bias in the western equatorial Indian Ocean, accompanied by the east-west mean sea level pressure gradient over the Indian Ocean. Furthermore, this study finds local wind-induced Ekman pumping to be a more dominant factor in thermocline depth bias than Rossby waves, despite CMIP6 models replicating Rossby wave propagation. This study highlights the importance of the beta term in the Ekman pumping simulation. Thus, reducing the boreal summer-to-fall easterly wind bias over the Indian Ocean region may improve the thermocline depth simulation over the SCTR region.
Streptomyces as a promising biological control agents for plant pathogens
Authors: Khan Shaista , Srivastava Seweta , Karnwal Arun , Malik Tabarak
Published: November 2023

Plant diseases caused by pathogenic microorganisms in agriculture present a considerable obstacle, resulting in approximately 30–40% crop damage. The use of conventional techniques to manage these microorganisms, i.e., applying chemical pesticides and antimicrobials, has been discovered to have adverse effects on human health and the environment. Furthermore, these methods have contributed to the emergence of resistance among phytopathogens. Consequently, it has become imperative to investigate natural alternatives to address this issue. The Streptomyces genus of gram-positive bacteria is a potentially viable natural alternative that has been extensively researched due to its capacity to generate diverse antimicrobial compounds, such as metabolites and organic compounds. Scientists globally use diverse approaches and methodologies to extract new bioactive compounds from these bacteria. The efficacy of bioactive compounds in mitigating various phytopathogens that pose a significant threat to crops and plants has been demonstrated. Hence, the Streptomyces genus exhibits potential as a biological control agent for combating plant pathogens. This review article aims to provide further insight into the Streptomyces genus as a source of antimicrobial compounds that can potentially be a biological control against plant pathogens. The investigation of various bioactive compounds synthesized by this genus can enhance our comprehension of their prospective utilization in agriculture.
Acetylcholine facilitates localized synaptic potentiation and location specific feature binding
Authors: Yang Yihao , Booth Victoria , Zochowski Michal
Published: November 2023

Forebrain acetylcholine (ACh) signaling has been shown to drive attention and learning. Recent experimental evidence of spatially and temporally constrained cholinergic signaling has sparked interest to investigate how it facilitates stimulus-induced learning. We use biophysical excitatory-inhibitory (E-I) multi-module neural network models to show that external stimuli and ACh signaling can mediate spatially constrained synaptic potentiation patterns. The effects of ACh on neural excitability are simulated by varying the conductance of a muscarinic receptor-regulated hyperpolarizing slow K+ current (m-current). Each network module consists of an E-I network with local excitatory connectivity and global inhibitory connectivity. The modules are interconnected with plastic excitatory synaptic connections, that change via a spike-timing-dependent plasticity (STDP) rule. Our results indicate that spatially constrained ACh release influences the information flow represented by network dynamics resulting in selective reorganization of inter-module interactions. Moreover the information flow depends on the level of synchrony in the network. For highly synchronous networks, the more excitable module leads firing in the less excitable one resulting in strengthening of the outgoing connections from the former and weakening of its incoming synapses. For networks with more noisy firing patterns, activity in high ACh regions is prone to induce feedback firing of synchronous volleys and thus strengthening of the incoming synapses to the more excitable region and weakening of outgoing synapses. Overall, these results suggest that spatially and directionally specific plasticity patterns, as are presumed necessary for feature binding, can be mediated by spatially constrained ACh release.
Competing endogenous RNAs in human astrocytes: crosstalk and interacting networks in response to lipotoxicity
Authors: Gil-Jaramillo Natalia , Aristizábal-Pachón Andrés Felipe , Luque Aleman María Alejandra , González Gómez Valentina , Escobar Hurtado Hans Deyvy , Girón Pinto Laura Camila , Jaime Camacho Juan Sebastian , Rojas-Cruz Alexis Felipe , González-Giraldo Yeimy , Pinzón Andrés , González Janneth
Published: November 2023

Neurodegenerative diseases (NDs) are characterized by a progressive deterioration of neuronal function, leading to motor and cognitive damage in patients. Astrocytes are essential for maintaining brain homeostasis, and their functional impairment is increasingly recognized as central to the etiology of various NDs. Such impairment can be induced by toxic insults with palmitic acid (PA), a common fatty acid, that disrupts autophagy, increases reactive oxygen species, and triggers inflammation. Although the effects of PA on astrocytes have been addressed, most aspects of the dynamics of this fatty acid remain unknown. Additionally, there is still no model that satisfactorily explains how astroglia goes from being neuroprotective to neurotoxic. Current incomplete knowledge needs to be improved by the growing field of non-coding RNAs (ncRNAs), which is proven to be related to NDs, where the complexity of the interactions among these molecules and how they control other RNA expressions need to be addressed. In the present study, we present an extensive competing endogenous RNA (ceRNA) network using transcriptomic data from normal human astrocyte (NHA) cells exposed to PA lipotoxic conditions and experimentally validated data on ncRNA interaction. The obtained network contains 7 lncRNA transcripts, 38 miRNAs, and 239 mRNAs that showed enrichment in ND-related processes, such as fatty acid metabolism and biosynthesis, FoxO and TGF-β signaling pathways, prion diseases, apoptosis, and immune-related pathways. In addition, the transcriptomic profile was used to propose 22 potential key controllers lncRNA/miRNA/mRNA axes in ND mechanisms. The relevance of five of these axes was corroborated by the miRNA expression data obtained in other studies. MEG3 (ENST00000398461)/hsa-let-7d-5p/ATF6B axis showed importance in Parkinson’s and late Alzheimer’s diseases, while AC092687.3/hsa-let-7e-5p/[SREBF2, FNIP1, PMAIP1] and SDCBP2-AS1 (ENST00000446423)/hsa-miR-101-3p/MAPK6 axes are probably related to Alzheimer’s disease development and pathology. The presented network and axes will help to understand the PA-induced mechanisms in astrocytes, leading to protection or injury in the CNS under lipotoxic conditions as part of the intricated cellular regulation influencing the pathology of different NDs. Furthermore, the five corroborated axes could be considered study targets for new pharmacologic treatments or as possible diagnostic molecules, contributing to improving the quality of life of millions worldwide.
Case Report: Multidisciplinary management of primary inferior vena cava leiomyosarcoma: a comprehensive case study
Authors: Li Wuchao , Zhang Xiaoyong , Zhang Yi , Wang Rongpin
Published: November 2023

Introduction

Primary Inferior vena cava (IVC) leiomyosarcoma, a rare malignant tumor, presents unique challenges in diagnosis and treatment due to its rarity and the lack of consensus on surgical and adjuvant therapy approaches.

Case Report

A 39-year-old female patient presented with lower limb swelling and mild fatigue. Contrast-enhanced CT identified a tumor mass within the dilated IVC. Abdominal MRI revealed primary IVC leiomyosarcoma extending into the right hepatic vein. A multidisciplinary consultation established a diagnosis and devised a treatment plan, opting for Ex-vivo Liver Resection and Auto-transplantation (ELRA), tumor resection and IVC reconstruction. Pathological examination confirmed primary IVC leiomyosarcoma. Postoperatively, the patient underwent a comprehensive treatment strategy that included radiochemotherapy, immunotherapy, targeted therapy, and PRaG therapy (PD-1 inhibitor, Radiotherapy, and Granulocyte-macrophage colony-stimulating factor). Despite the tumor’s recurrence and metastasis, the disease progression was partially controlled.

Conclusion

This case report emphasizes the complexities of diagnosing and treating IVC leiomyosarcoma and highlights the potential benefits of employing ELRA, IVC reconstruction, and PRaG therapy. Our study may serve as a valuable reference for future investigations addressing the management of this rare disease.
The combination of baseline neutrophil to lymphocyte ratio and dynamic changes during treatment can better predict the survival of osteosarcoma patients
Authors: Li Longqing , Li Ye , Lu Minxun , Wang Yitian , Li Zhuangzhuang , Hu Xin , He Xuanhong , Gong Taojun , Luo Yi , Zhou Yong , Min Li , Tu Chongqi
Published: November 2023

Background

Osteosarcoma is a primary malignant bone tumor with a high metastatic potential that accounts for a significant proportion of all bone tumors. The prognosis for patients with metastatic or recurrence disease remains poor. The neutrophil-to-lymphocyte ratio (NLR) has become a potential prognostic biomarker for cancer. Recent evidence suggests that the dynamic changes in neutrophil-to-lymphocyte ratio (NLR) during treatment may be more informative in predicting patient prognosis, but the value of dynamic NLR in osteosarcoma has not yet been determined.

Methods

This retrospective study retrospectively analyzed the clinical information of 251 osteosarcoma patients diagnosed and treated in West China Hospital of Sichuan University, explored the impact of baseline NLR and changes in NLR during treatment on the prognosis of osteosarcoma patients, and further combined baseline NLR with Delta NLR to build an NLR staging system.

Results

The results showed that both baseline NLR and delta NLR had some predictive ability for the prognosis of osteosarcoma patients (P = 6.90e-4, P = 0.022). Patients with high baseline NLR were more likely to have a decrease in delta NLR (P = 1.24e-10). The NLR stage had a better predictive ability than baseline NLR and delta NLR, and was an independent prognostic factor for overall survival in osteosarcoma patients HR: 2.456 (1.625-3.710) (P = 1.97e-05).

Conclusion

NLR has value in continuous monitoring, and continuous monitoring of NLR can better predict the survival of osteosarcoma patients compared to baseline NLR.
Help overcoming pain early, a brief person-centred intervention for adolescents with chronic pain in a school setting, may improve symptoms of insomnia
Authors: Wallbing Ulrika , Nilsson Stefan , Lundberg Mari , Wigert Helena , Kemani Mike K.
Published: November 2023

Introduction and aims

Chronic pain and symptoms of insomnia affect large numbers of adolescents and early interventions are prioritized. The aim of the current study was to evaluate potential secondary effects of the intervention, Help Overcoming Pain Early (HOPE), on symptoms of insomnia and self-rated health.

Methods

The study included non-randomized aggregated data from the active and control conditions in a previously conducted randomized controlled trial evaluating the efficacy of HOPE, after the participants in the control condition also had received the intervention. Symptoms of insomnia were assessed with the Minimal Insomnia Symptom Scale and self-rated health was assessed with one item, at the start of the intervention, post intervention, and at a six-month follow-up. Baseline variables included age, gender, pain localization, pain impact, school absence and symptoms of depression (assessed with the Center for Epidemiological Studies Depression Scale for Children). Inferential analyzes were performed using Linear Mixed Models (LMM). Effect sizes were evaluated by calculating Cohen's d.

Results

There were statistically significant improvements in symptoms of insomnia at the six-month follow-up, and statistically significant improvements in self-rated health at the end of the intervention and at the six-month follow-up. Effect sizes were small across outcomes and assessments.

Discussion and conclusion

Results illustrated significant but small improvements in symptoms of insomnia and self-rated health in adolescents with chronic pain following the HOPE intervention. Although caution is needed when assessing the findings, results illustrate the potential utility of an accessible brief early intervention in a school context.