bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeted Microchip Capillary Electrophoresis-Orbitrap Mass Spectrometry Metabolomics to Monitor Ovarian Cancer Progression
Authors: Sah Samyukta , Yun Sylvia R. , Gaul David A. , Botros Andro , Park Eun Young , Kim Olga , Kim Jaeyeon , Fernández Facundo M.
Published: June 2022

The lack of effective screening strategies for high-grade serous carcinoma (HGSC), a subtype of ovarian cancer (OC) responsible for 70–80% of OC related deaths, emphasizes the need for new diagnostic markers and a better understanding of disease pathogenesis. Capillary electrophoresis (CE) coupled with high-resolution mass spectrometry (HRMS) offers high selectivity and sensitivity for ionic compounds, thereby enhancing biomarker discovery. Recent advances in CE-MS include small, chip-based CE systems coupled with nanoelectrospray ionization (nanoESI) to provide rapid, high-resolution analysis of biological specimens. Here, we describe the development of a targeted microchip (µ) CE-HRMS method, with an acquisition time of only 3 min and sample injection volume of 4nL, to analyze 40 target metabolites in serum samples from a triple-mutant (TKO) mouse model of HGSC. Extracted ion electropherograms showed sharp, baseline resolved peak shapes, even for structural isomers such as leucine and isoleucine. All calibration curves of the analytes maintained good linearity with an average R2 of 0.994, while detection limits were in the nM range. Thirty metabolites were detected in mouse serum with recoveries ranging from 78 to 120%, indicating minimal ionization suppression and good accuracy. We applied the µCE-HRMS method to biweekly-collected serum samples from TKO and TKO control mice. A time-resolved analysis revealed characteristic temporal trends for amino acids, nucleosides, and amino acid derivatives. These metabolic alterations are indicative of altered nucleotide biosynthesis and amino acid metabolism in HGSC development and progression. A comparison of the µCE-HRMS dataset with non-targeted ultra-high performance liquid chromatography (UHPLC)–MS results showed identical temporal trends for the five metabolites detected with both platforms, indicating the µCE-HRMS method performed satisfactorily in terms of capturing metabolic reprogramming due to HGSC progression while reducing the total data collection time three-fold.
Influence of Radiation-Induced Displacement Defect in 1.2 kV SiC Metal-Oxide-Semiconductor Field-Effect Transistors
Authors: Lee Gyeongyeop , Ha Jonghyeon , Kim Kihyun , Bae Hagyoul , Kim Chong-Eun , Kim Jungsik
Published: June 2022

The effect of displacement defect on SiC metal-oxide-semiconductor field-effect transistors (MOSFETs) due to radiation is investigated using technology computer-aided design (TCAD) simulation. The position, energy level, and concentration of the displacement defect are considered as variables. The transfer characteristics, breakdown voltage, and energy loss of a double-pulse switching test circuit are analyzed. Compared with the shallow defect energy level, the deepest defect energy level with EC − 1.55 eV exhibits considerable degradation. The on-current decreases by 54% and on-resistance increases by 293% due to the displacement defect generated at the parasitic junction field-effect transistor (JFET) region next to the P-well. Due to the existence of a defect in the drift region, the breakdown voltage increased up to 21 V. In the double-pulse switching test, the impact of displacement defect on the power loss of SiC MOSFETs is negligible.
Editorial for the Special Issue on Micromachines for Non-Newtonian Microfluidics
Authors: Mei Lanju , Qian Shizhi
Published: June 2022

Microfluidics has seen a remarkable growth over the past few decades, with its extensive applications in engineering, medicine, biology, chemistry, etc [...]
A Highly Accurate Method for Deformation Reconstruction of Smart Deformable Structures Based on Flexible Strain Sensors
Authors: Yu Chengguo , Gao Xinyu , Liao Wenlin , Zhang Zhili , Wang Guishan
Published: June 2022

Smart deformable structures that integrate designing, sensing, and controlling technology have been widely applied in the fields of aerospace, robotics, and biomedical engineering due to their multi-functional requirements. The deformation reconstruction method essential for security monitoring and shape controlling, especially for the large deflection deformation, remains a challenge on accuracy and efficiency. This paper takes a wind tunnel’s fixed-flexible nozzle (FFN) plate as the research object to develop a highly accurate deformation reconstruction method based on sensing information from flexible strain sensors. The mechanical behaviors of the FFN plate with large deflection deformation, which is modeled as a cantilever beam, are studied to analyze the relationship of the strain and moment. Furthermore, the large deflection factor and shell bending theory are creatively utilized to derive and modify the strain–moment based reconstruction method (SMRM), where the contour of the FFN plate is solved by particular elliptic integrals. As a result, structural simulation based on ABAQUS further demonstrates that the reconstruction error of SMRM is 21.13% less than that of the classic Ko-based reconstruction method (KORM). An FFN prototype accompanied by customized flexible sensors is developed to evaluate the accuracy and efficiency of the SMRM, resulting in a maximum relative error of 3.97% that is acceptable for practical applications in smart deformable structures, not limited to the FFN plate.
Bending and Vibration Analysis of Flexoelectric Beam Structure on Linear Elastic Substrates
Authors: Zhang Maomao , Zhou Zhidong
Published: June 2022

With the development of micro-nanotechnology, smart electronic devices are being updated and developed, and more and more flexoelectric sensors, actuators, and energy harvesters attached to elastic substrates have attracted a surge of interest due to unique features at the nano-scale. In this paper, the static bending behavior and vibration characteristics of a flexoelectric beam structure based on a linear elastic substrate under a magnetic field environment are investigated. Based on the electrical Gibbs free energy density, the governing equations and boundary conditions of structures are derived by using the Euler–Bernoulli beam theory and the Hamilton’s variational principle. The expressions of the deflection and the induced electric potential of the beam structure are expressed analytically. The natural frequency of the beam under the open-circuit electrical conditions with surface electrodes (OCI) are obtained after further extending the solution. The results show that the flexoelectric effect, the linear elastic substrate, and the magnetic field have significant effects on the static bending and vibration behaviors of the flexoelectric beam which are beneficial for designing and developing flexoelectric devices with elastic substrates.
Highly Porous Holey Carbon for High Areal Energy Density Solid-State Supercapacitor Application
Authors: Young Christine , Chen Hong-Ting , Guo Sahn-Ze
Published: June 2022

Biomass materials are perceived as sustainable, carbon-rich precursors for the fabrication of carbon materials. In this study, we demonstrated the capacitance performance of biomass-derived carbon, produced by using golden shower tree seeds (GTs) as carbon precursors and potassium ferrate (K2FeO4) as the activation agent. The as-prepared porous carbon (GTPC) possessed an ultrahigh specific surface area (1915 m2 g−1) and abundant pores. They also exhibited superior electrochemical performance, owing to their well-constructed porous structure, high surface area, and optimized porous structure. Optimized activated carbon (GTPC-1) was used to assemble a symmetric solid-state supercapacitor device with poly(vinyl alcohol) (PVA)/H2SO4 as a solid-state gel electrolyte. The device exhibited a maximum areal energy density of 42.93 µWh cm−2 at a power density of 520 µW cm−2.
A Reconfigurable Surface-Plasmon-Based Filter/Sensor Using D-Shaped Photonic Crystal Fiber
Authors: Selvendran S. , Divya J. , Sivanantha Raja A. , Sivasubramanian A. , Itapu Srikanth
Published: June 2022

A reconfigurable surface-plasmon-based filter/sensor using D-shaped photonic crystal fiber is proposed. Initially a D-shaped PCF is designed and optimized to realize the highly birefringence and by ensuring the single polarization filter. A tiny layer of silver is placed on the flat surface of the D-shaped fiber with a small half-circular opening to activate the plasmon modes. By the surface plasmon effect a maximum confinement loss of about 713 dB/cm is realized at the operating wavelength of 1.98 µm in X-polarized mode. At this wavelength the proposed fiber only allows Y-polarization and filters the X-polarization using surface plasmon resonance. It is also exhibiting maximum confinement loss of about 426 dB/cm at wavelength 1.92 µm wavelength for Y-polarization. At this 1.92 µm wavelength the proposed structure attenuated the Y-polarization completely and allowed X-polarization alone. The proposed PCF polarization filter can be extended as a sensor by adding an analyte outside this filter structure. The proposed sensor can detect even a small refractive index (RI) variation of analytes ranging from 1.34–1.37. This sensor provides the maximum sensitivity of about 5000 nm/RIU; it enables this sensor to be ideally suited for various biosensing and industrial applications.
Sensitivity Enhancement of Group Refractive Index Biosensor through Ring-Down Interferograms of Microring Resonator
Authors: Lai Hsuan , Kuo Tzu-Ning , Xu Jia-Yi , Hsu Shih-Hsiang , Hsu Yi-Cheng
Published: June 2022

In recent years, silicon-on-insulator substrates have been utilized for high-speed and low-power electronic components. Because of the high refractive index contrast of the silicon wire, its photonic device footprint can be significantly reduced. Moreover, the silicon photonic process is compatible with a complementary metal-oxide-semiconductor fabrication, which will benefit the high-density optoelectronic integrated circuits development. Researchers have recently proposed using the microring resonator (MRR) for label-free biosensing applications. The high-quality factor caused by the substantial electric field enhancement within the ring makes the MRR a good candidate for biomolecule detection under low analyte concentration conditions. This paper proposes an MRR chip to be a biosensor on the silicon platform through the relative displacement between the spatial ring-down interferograms at various cladding layers. The higher-order ring-down of the spatial interference wave packet will enhance the biosensing sensitivity after optimizing the coupling, MRR length, and the optical source bandwidth at the fixed optical waveguide loss. Finally, a typical sensitivity of 642,000 nm per refractive index unit is demonstrated under 0.1 μW minimum optical power detection for an MRR with a 100 μm radius. Higher sensitivity can be executed by a narrow bandwidth and lower silicon wire propagation loss.
Motor Imagery EEG Classification Based on Transfer Learning and Multi-Scale Convolution Network
Authors: Chang Zhanyuan , Zhang Congcong , Li Chuanjiang
Published: June 2022

For the successful application of brain-computer interface (BCI) systems, accurate recognition of electroencephalography (EEG) signals is one of the core issues. To solve the differences in individual EEG signals and the problem of less EEG data in classification and recognition, an attention mechanism-based multi-scale convolution network was designed; the transfer learning data alignment algorithm was then introduced to explore the application of transfer learning for analyzing motor imagery EEG signals. The data set 2a of BCI Competition IV was used to verify the designed dual channel attention module migration alignment with convolution neural network (MS-AFM). Experimental results showed that the classification recognition rate improved with the addition of the alignment algorithm and adaptive adjustment in transfer learning; the average classification recognition rate of nine subjects was 86.03%.
Understanding the Mechanisms of SiC–Water Reaction during Nanoscale Scratching without Chemical Reagents
Authors: Cheng Zhihao , Luo Qiufa , Lu Jing , Tian Zige
Published: June 2022

Microcracks inevitably appear on the SiC wafer surface during conventional thinning. It is generally believed that the damage-free surfaces obtained during chemical reactions are an effective means of inhibiting and eliminating microcracks. In our previous study, we found that SiC reacted with water (SiC–water reaction) to obtain a smooth surface. In this study, we analyzed the interfacial interaction mechanisms between a 4H-SiC wafer surface (0001-) and diamond indenter during nanoscale scratching using distilled water and without using an acid–base etching solution. To this end, experiments and ReaxFF reactive molecular dynamics simulations were performed. The results showed that amorphous SiO2 was generated on the SiC surface under the repeated mechanical action of the diamond abrasive indenter during the nanoscale scratching process. The SiC–water reaction was mainly dependent on the load and contact state when the removal size of SiC was controlled at the nanoscale and the removal mode was controlled at the plastic stage, which was not significantly affected by temperature and speed. Therefore, the reaction between water and SiC on the wafer surface could be controlled by effectively regulating the load, speed, and contact area. Microcracks can be avoided, and damage-free thinning of SiC wafers can be achieved by controlling the SiC–water reaction on the SiC wafer surface.
Screw Analysis, Modeling and Experiment on the Mechanics of Tibia Orthopedic with the Ilizarov External Fixator
Authors: Su Peng , Wang Sikai , Lai Yuliang , Zhang Qinran , Zhang Leiyu
Published: June 2022

The Ilizarov external fixator plays an important role in the correction of complex malformed limbs. Our purpose in this work was to reveal the transmission of adjustable forces between the external fixator and the broken bone, and express the stress distribution at the end of the broken bone during the orthopedic treatment. Firstly, the screw model of the fixator was established and the theoretical relationship between the adjustable force and the stress was obtained. A sheep tibia was taken as a representative research object and its ediTable 3D entity was obtained by CT scanning. Then the mechanical model of the fixator and tibia was built using the ABAQUS software. Correction experiments were performed on the sheep tibia to measure the adjustable/support forces and tensions of the tibia. The measured results were imported to the screw and mechanical model, and the theoretical and simulation values were calculated. The theoretical tensions calculated by the screw model had a similar shape and doubled the value compared with that of the measured results. The transfer efficiency between the two results was improved and kept at about 50% after the initial 2~3 periods. The maximum stress occurring at the surface of the broken bone end was near the Kirschner wire pinhole. The simulation results for the tensions from the mechanical model showed a similar change trend, and the value was slightly higher. A biomechanical model of the Ilizarov external fixator was derived and verified through calculations, simulations and experiments. The change law of the adjustable forces and the tensions existing in the broken sheep tibias is presented herein, and offers a helpful contribution to orthopedic treatment.
Functional Characterisation of Two Novel Deacetylases from Streptococcus pyogenes
Authors: Aspell Tiger , Khemlani Adrina Hema Jethanand , Loh Jacelyn Mei San , Tsai Catherine Jia-Yun , Proft Thomas
Published: June 2022

Streptococcus pyogenes (Group A Streptococcus, GAS) is an exclusively human pathogen that causes a wide range of diseases. We have identified two novel proteins, Spy1094 and Spy1370, which show sequence similarity with peptidoglycan deacetylases (PGDAs) from other streptococcal species like S. pneumoniae and S. iniae, that represent important virulence factors. Recombinant Spy1094 and Spy1370 were active at a wide pH range (pH 4.0–9.0) and showed metal ion-dependence, with the highest activities observed in the presence of Mn2+, Mg2+and Zn2+. The enzymes showed typical Michaelis–Menten saturation kinetics with the pseudo-substrate GlcNAc3. Binding affinities for rSpy1094 and rSpy1370 were high (Km = 2.2 ± 0.9 μM and 3.1 ± 1.1 μM, respectively), but substrate turnover was low (Kcat = 0.0075/s and 0.0089/s, respectively) suggesting that peptidoglycan might not be the preferred target for deacetylation. Both enzymes were expressed during bacterial growth.
Culturable Bacterial Diversity from the Basaltic Subsurface of the Young Volcanic Island of Surtsey, Iceland
Authors: Bergsten Pauline , Vannier Pauline , Frion Julie , Mougeolle Alan , Marteinsson Viggó Þór
Published: June 2022

The oceanic crust is the world’s largest and least explored biosphere on Earth. The basaltic subsurface of Surtsey island in Iceland represents an analog of the warm and newly formed-oceanic crust and offers a great opportunity for discovering novel microorganisms. In this study, we collected borehole fluids, drill cores, and fumarole samples to evaluate the culturable bacterial diversity from the subsurface of the island. Enrichment cultures were performed using different conditions, media and temperatures. A total of 195 bacterial isolates were successfully cultivated, purified, and identified based on MALDI-TOF MS analysis and by 16S rRNA gene sequencing. Six different clades belonging to Firmicutes (40%), Gammaproteobacteria (28.7%), Actinobacteriota (22%), Bacteroidota (4.1%), Alphaproteobacteria (3%), and Deinococcota (2%) were identified. Bacillus (13.3%) was the major genus, followed by Geobacillus (12.33%), Enterobacter (9.23%), Pseudomonas (6.15%), and Halomonas (5.64%). More than 13% of the cultured strains potentially represent novel species based on partial 16S rRNA gene sequences. Phylogenetic analyses revealed that the isolated strains were closely related to species previously detected in soil, seawater, and hydrothermal active sites. The 16S rRNA gene sequences of the strains were aligned against Amplicon Sequence Variants (ASVs) from the previously published 16S rRNA gene amplicon sequence datasets obtained from the same samples. Compared with the culture-independent community composition, only 5 out of 49 phyla were cultivated. However, those five phyla accounted for more than 80% of the ASVs. Only 121 out of a total of 5642 distinct ASVs were culturable (≥98.65% sequence similarity), representing less than 2.15% of the ASVs detected in the amplicon dataset. Here, we support that the subsurface of Surtsey volcano hosts diverse and active microbial communities and that both culture-dependent and -independent methods are essential to improving our insight into such an extreme and complex volcanic environment.
Computational Identification of Potential Multitarget Inhibitors of Nipah Virus by Molecular Docking and Molecular Dynamics
Authors: Randhawa Vinay , Pathania Shivalika , Kumar Manoj
Published: June 2022

Nipah virus (NiV) is a recently emerged paramyxovirus that causes severe encephalitis and respiratory diseases in humans. Despite the severe pathogenicity of this virus and its pandemic potential, not even a single type of molecular therapeutics has been approved for human use. Considering the role of NiV attachment glycoprotein G (NiV-G), fusion glycoprotein (NiV-F), and nucleoprotein (NiV-N) in virus replication and spread, these are the most attractive targets for anti-NiV drug discovery. Therefore, to prospect for potential multitarget chemical/phytochemical inhibitor(s) against NiV, a sequential molecular docking and molecular-dynamics-based approach was implemented by simultaneously targeting NiV-G, NiV-F, and NiV-N. Information on potential NiV inhibitors was compiled from the literature, and their 3D structures were drawn manually, while the information and 3D structures of phytochemicals were retrieved from the established structural databases. Molecules were docked against NiV-G (PDB ID:2VSM), NiV-F (PDB ID:5EVM), and NiV-N (PDB ID:4CO6) and then prioritized based on (1) strong protein-binding affinity, (2) interactions with critically important binding-site residues, (3) ADME and pharmacokinetic properties, and (4) structural stability within the binding site. The molecules that bind to all the three viral proteins (NiV-G ∩ NiV-F ∩ NiV-N) were considered multitarget inhibitors. This study identified phytochemical molecules RASE0125 (17-O-Acetyl-nortetraphyllicine) and CARS0358 (NA) as distinct multitarget inhibitors of all three viral proteins, and chemical molecule ND_nw_193 (RSV604) as an inhibitor of NiV-G and NiV-N. We expect the identified compounds to be potential candidates for in vitro and in vivo antiviral studies, followed by clinical treatment of NiV.
Combining Desirable Traits for a Good Biocontrol Strategy against Sclerotinia sclerotiorum
Authors: Albert Daphné , Dumonceaux Tim , Carisse Odile , Beaulieu Carole , Filion Martin
Published: June 2022

The fungal pathogen Sclerotinia sclerotiorum (Helotiales: Sclerotiniaceae) causes white mold, a disease that leads to substantial losses on a wide variety of hosts throughout the world. This economically important fungus affects yield and seed quality, and its control mostly relies on the use of environmentally damaging fungicides. This review aimed to present the latest discoveries on microorganisms and the biocontrol mechanisms used against white mold. A special focus is put on the identification of biocontrol desirable traits required for efficient disease control. A better understanding of the mechanisms involved and the conditions required for their action is also essential to ensure a successful implementation of biocontrol under commercial field conditions. In this review, a brief introduction on the pathogen, its disease cycle, and its main pathogenicity factors is presented, followed by a thorough description of the microorganisms that have so far demonstrated biocontrol potential against white mold and the mechanisms they use to achieve control. Antibiosis, induced systemic resistance, mycoparasitism, and hypovirulence are discussed. Finally, based on our actual knowledge, the best control strategies against S. sclerotiorum that are likely to succeed commercially are discussed, including combining biocontrol desirable traits of particular interest.
Differences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in Campylobacter jejuni and Campylobacter coli
Authors: Hanafy Zahra , Osborne Jason A. , Miller William G. , Parker Craig T. , Olson Jonathan W. , Jackson James H. , Kathariou Sophia
Published: June 2022

Campylobacter jejuni and Campylobacter coli are leading zoonotic foodborne pathogens, and the drugs of choice for human campylobacteriosis are macrolides (e.g., erythromycin) and fluoroquinolones. C. jejuni and C. coli are naturally competent for transformation via naked DNA uptake, but potential differences in transformation frequency (TF) for different antimicrobial resistance (AMR) markers remain poorly understood. We determined TFs for resistance to different antibiotics using as recipient a derivative of C. jejuni NCTC 11168 (strain SN:CM) with donor DNA from multidrug-resistant C. jejuni or C. coli. TF for nalidixic acid resistance ranked significantly highest (~1.4 × 10−3), followed by resistance to streptomycin and gentamicin. Tetracycline resistance via chromosomal tet(O) was less commonly transferred (~7.6 × 10−7), while transformation to erythromycin resistance was rare (≤4.7 × 10−8). We also determined TFs with the contemporary poultry-derived strains C. jejuni FSIS 11810577 and C. coli FSIS 1710488 as recipients. TFs to nalidixic acid and streptomycin resistance remained the highest (~7 × 10−4). However, TF for gentamicin resistance was remarkably low in certain recipient–donor combinations, while average TF for erythromycin resistance was noticeably higher (~3 × 10−6) than with SN:CM. Findings from this experimental model provide insights into factors that may impact transformation-mediated transfer of AMR leading to AMR dissemination in the agricultural ecosystem.
Role of Marine Bacterial Contaminants in Histamine Formation in Seafood Products: A Review
Authors: Oktariani Adnorita Fandah , Ramona Yan , Sudaryatma Putu Eka , Dewi Ida Ayu Mirah Meliana , Shetty Kalidas
Published: June 2022

Histamine is a toxic biogenic amine commonly found in seafood products or their derivatives. This metabolite is produced by histamine-producing bacteria (HPB) such as Proteus vulgaris, P. mirabilis, Enterobacter aerogenes, E. cloacae, Serratia fonticola, S. liquefaciens, Citrobacter freundii, C. braakii, Clostridium spp., Raoultella planticola, R. ornithinolytica, Vibrio alginolyticus, V. parahaemolyticus, V. olivaceus, Acinetobacter lowffi, Plesiomonas shigelloides, Pseudomonas putida, P. fluorescens, Aeromonas spp., Photobacterium damselae, P. phosphoreum, P. leiognathi, P. iliopiscarium, P. kishitanii, and P. aquimaris. In this review, the role of these bacteria in histamine production in fish and seafood products with consequences for human food poisoning following consumption are discussed. In addition, methods to control their activity in countering histamine production are proposed.
Investigation of the Phageome and Prophages in French Cider, a Fermented Beverage
Authors: Ledormand Pierre , Desmasures Nathalie , Midoux Cédric , Rué Olivier , Dalmasso Marion
Published: June 2022

Phageomes are known to play a key role in the functioning of their associated microbial communities. The phageomes of fermented foods have not been studied thoroughly in fermented foods yet, and even less in fermented beverages. Two approaches were employed to investigate the presence of phages in cider, a fermented beverage made from apple, during a fermentation process of two cider tanks, one from an industrial producer and one from a hand-crafted producer. The phageome (free lytic phages) was explored in cider samples with several methodological developments for total phage DNA extraction, along with single phage isolation. Concentration methods, such as tangential flow filtration, flocculation and classical phage concentration methods, were employed and tested to extract free phage particles from cider. This part of the work revealed a very low occurrence of free lytic phage particles in cider. In parallel, a prophage investigation during the fermentation process was also performed using a metagenomic approach on the total bacterial genomic DNA. Prophages in bacterial metagenomes in the two cider tanks seemed also to occur in low abundance, as a total of 1174 putative prophages were identified in the two tanks overtime, and only two complete prophages were revealed. Prophage occurrence was greater at the industrial producer than at the hand-crafted producer, and different dynamics of prophage trends were also observed during fermentation. This is the first report dealing with the investigation of the phageome and of prophages throughout a fermentation process of a fermented beverage.
Dissolution Behavior of Sodium Phosphate in a Na3PO4–Na2WO4–NaOH Solution System
Authors: He Bing-Xuan , Liang Yong , Fan Ze-Kun , Xu Lue-Wei , Liu De-Gang , Xu Guo-Zuan
Published: June 2022

Sodium hydroxide autoclaving is the main method for smelting scheelite in China. In this method, sodium phosphate is added as an additive to realize the highly efficient decomposition of scheelite, and a crude sodium tungstate solution containing sodium phosphate and sodium hydroxide is obtained. In the subsequent process of ion exchange, phosphorus ions in the solution compete with the resin adsorption of tungstate, which reduces the adsorption capacity of the resin and endangers the purity of the subsequent sodium tungstate solution. To remove the phosphorus from crude sodium tungstate solution, a chemical purification method is usually adopted. The principle of the chemical purification method is to use chemical reagents to react with impurities to form precipitates to achieve the purpose of impurity removal. Because of the advantages of simple industrial implementation and high impurity removal efficiency, it has been widely used in phosphorus removal from crude sodium tungstate solution. However, in the process of phosphate removal in a crude sodium phosphate solution, the chemical purification method has some disadvantages. First, the additional cost of chemical reagents is required, and other metal impurities from chemical reagents would be introduced to crude sodium tungstate solution. Second, phosphate impurity removed by the chemical precipitation method is usually sedimented in other forms but sodium phosphate, which makes the phosphate resource unable to be recycled for tungsten smelting. Therefore, a novel phosphorus removal method needs to be developed. The dissolution behavior of sodium phosphate in a Na3PO4–Na2WO4–NaOH system was investigated in this paper. The results showed that in binary or ternary solution systems of sodium phosphate, sodium tungstate, and sodium hydroxide, the common-ion effect and salt effect exist simultaneously. The common-ion effect decreases the solubility of sodium phosphate, while the salt effect increases the solubility of sodium phosphate. Increasing the concentration of sodium hydroxide or sodium tungstate and lowering the temperature of the solution can greatly reduce the phosphorus concentration in crude sodium tungstate solution, making the crude sodium tungstate solution meet industrial requirements of ion exchange. The results of the study lay a theoretical foundation for the development of new phosphorus removal methods.
LA-ICP-MS Mapping of Barren Sandstone from the Proterozoic Athabasca Basin (Canada)—Footprint of U- and REE-Rich Basinal Fluids
Authors: Chi Guoxiang , Potter Eric , Petts Duane , Jackson Simon , Chu Haixia
Published: June 2022

The Proterozoic Athabasca Basin hosts a large number of high-grade, large-tonnage unconformity-related uranium (U) deposits, many of which are also enriched in rare earth elements (REE). The basin also contains hydrothermal REE mineralization unassociated with U. Previous studies postulated that U and REE were derived from either the basin or the basement; however, the exact source of the metals remains ambiguous. This study provides evidence of U- and REE-rich fluids throughout the Athabasca Basin through laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) mapping of barren sandstone distal to mineralized areas. The results indicate that elevated U and REE concentrations mainly occur in the matrix; there are strong positive correlations between U and REE, Th, P and Sr, and moderate positive correlations between U and Zr, Ba, Fe, Al, K and Ca, but the few spots with the highest U are unrelated to these elements. Quantitative evaluation of the element correlations, together with scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) analysis, suggests that most of the elevated U and REE are hosted in aluminum phosphate sulfate (APS) minerals rather than apatite and monazite. As the APS minerals are of diagenetic-hydrothermal origin, the results testify to the presence of U- and REE-rich fluids within the Athabasca Basin. The elevated Th/U ratio (~10) and REE pattern (strong heavy rare earth element (HREE) depletion) are consistent with a model in which large amounts of U and REE (especially HREE) were leached from the sandstone within the Athabasca Basin and contributed to U and REE mineralization near the unconformity between the sedimentary rocks in the basin and underlying basement rocks. This study demonstrates that LA-ICP-MS mapping can be effectively used to evaluate microscale distribution of elements and their mobility in sedimentary rocks to address mineralization related problems.
A Review of the G4 “Tin Granites” and Associated Mineral Occurrences in the Kivu Belt (Eastern Democratic Republic of the Congo) and Their Relationships with the Last Kibaran Tectono-Thermal Events
Authors: Villeneuve Michel , Wazi Nandefo , Kalikone Christian , Gärtner Andreas
Published: June 2022

The Mesoproterozoic Kibaran belts host large amounts of mineral resources such as cassiterite, wolframite, gold, and columbite-group minerals (“coltan”), all of them in high demand for new technologies and related industries. Most of these mineral occurrences are linked to the latest Mesoproterozoic to early Neoproterozoic G4 granitoid intrusions, also termed “tin(-bearing) granites”. Three main parts constitute the Kibaran belts: the Kibaride Belt (KIB) in the south, the Karagwe-Ankole Belt (KAB) in the east, and the Kivu Belt (KVB) in the west. Geological detail concerning the metallogeny of the KVB, which hosts large parts of these mineral resources, is very sparse. Previously, there was an assumed time gap of about 200 Ma between the formation of the last Kibaran terranes (1250 to 1200 Ma) and the emplacement of the G4 granites (ca. 1050 to 970 Ma), which generated the main mineralizations. Recent studies dated the last Kibaran tectono-thermal events younger than 1120 to 1110 Ma, which gave evidence for a drastic reduction in this time gap. Thus, the two newly recognized tectono-thermal events have likely contributed to the remobilization of older mineralized granites. These new data allow us to link the G4 granitoids and the associated mineralizations with the terminal Kibaran orogeny. However, the G4 emplacement and its relationships with older granites, with their host rocks and associated mineralizations, are not yet understood. Here, the main occurrences of the KVB are reviewed, and comparisons with similar mineralizations in the adjacent KAB are undertaken to improve our understanding on these complex relationships.
Closure of the Eastern Paleo-Asian Ocean: Constraints from the Age and Geochemistry of Early Permian Zhaojinggou Pluton in Inner Mongolia (North China)
Authors: Li Guang-Yao , Qiu Liang , Li Zhi-Dan , Gao Lei , Fu Chao , Wang Jia-Ying , Zhang Qi , Tu Jia-Run , Ge Teng-Fei
Published: June 2022

The closing time of the Paleo-Asian Ocean and the tectonic evolution of the northern margin of the North China Craton are still controversial. The geochronology and geochemistry of the Zhaojinggou monzogranite pluton provide new constraints on the late Paleozoic tectonic evolution and the closure time of the Paleo-Asian Ocean in the southern Central Asian Orogenic Belt (CAOB). The monzogranite yielded a zircon U-Pb age of 286.7 ± 1.2 Ma. Due to the characteristics of low–moderate Mg# values (25.87–39.21), low Fe2O3T values (1.13–1.72), and A/CNK > 1, we show that the pluton is weak peraluminous, high in potassium calc–alkaline series, and displays the feature of S-type granite. The total REE content is low, the distribution curve is right dipping, and the LREE is enriched; the δEu average value is 1.32 (1.11–1.54). The granite presents relatively high (87Sr/86Sr)i values of 0.712345–0.713723, low εNd(t) values of −8.89–−8.21 (an average value of −8.56), and a TDM2 of 1718–1773 Ma. Furthermore, the zircon in situ Hf isotopic analysis shows 176Hf/177Hf ratios of 0.282342 to 0.282614, low εHf(t) values of −9.27–0.38 (mean −4.74), and a TDM2 of 1275–1887 Ma. Additionally, high field strength elements such as Nb, Ta, and Ti are depleted, and large ion lithophile elements, e.g., Rb, Ba, K, and Sr, are enriched. The above features of the Zhaojinggou monzogranite indicate that the pluton was derived from late Paleoproterozoic to Mesoproterozoic lower crustal mafic materials. By discussing the genesis and tectonic implications of the pluton massif, we propose that the Zhaojinggou monzogranite represents a magmatic event caused by the crustal–mantle interaction during the southward subduction of the eastern Paleo-Asian Ocean in the northern margin of the North China Craton during the Early Permian.
Origin of the Bleaching in Lower Cretaceous Continental Red Beds in the Uragen Zn–Pb Deposit, Xinjiang, NW China, and Its Implications for Zn–Pb Mineralization
Authors: Gao Rongzhen , Xue Chunji , Dai Junfeng , Man Ronghao
Published: June 2022

The Uragen giant sandstone-hosted Zn–Pb deposit has a proven reserve of 5.90 Mt metals in the southern ore zone and potentially 10 Mt metals for the whole deposit, and orebodies are strictly confined to the bleached clastic rocks of the Lower Cretaceous red beds. The bleaching has been used to guide lead–zinc exploration; however, its nature and origin, as well as the relationship with Zn–Pb mineralization, remains unclear, although it is closely related to regional oil–gas infillings. Detailed field investigation and petrographic observation, TESCAN-integrated mineral analyzer (TIMA), and X-ray fluorescence (μ-XRF) analysis of the red and bleached sandstone at the same sedimentary layer demonstrate that the bleaching is mainly caused by the reductive dissolution of hematite pigment, which probably resulted from the interaction with H2S formed by in situ sulfate reduction during hydrocarbon migration. The calcite cements in the bleached sandstones show δ13C and δ18O values of −5.36~−5.94‰ and 20.94~27.91‰, respectively, and these samples fall close to the evolution line of decarboxylation of organic matter in δ13C-δ18O diagram, also suggesting a genetic relationship between the bleaching and hydrocarbon-bearing fluids. Petrol–mineral composition changes and sulfide characteristics of red, bleached, mineralized zones, as well as pyrite locally replaced by coarse-grained galena in the mineralized zone, imply that the bleaching may occurred before Zn–Pb mineralization. Mass balance calculation and μ-XRF analysis indicate that large amounts of Fe and minor Zn were extracted from red beds with little or no sulfates; however, the red beds with abundant sulfates may be a sink for leached ore metals during the bleaching process. We therefore propose that the former accumulations of iron sulfides and reduced sulfur in the bleached zones may provide an ideal chemical trap for later Zn–Pb mineralization, and the bleached zones with high ∑S contents are the favorable prospective targets of the Uragen-style sandstone-hosted Zn–Pb deposits.
MSWI Fly Ash Multiple Washing: Kinetics of Dissolution in Water, as Function of Time, Temperature and Dilution
Authors: Caviglia Caterina , Destefanis Enrico , Pastero Linda , Bernasconi Davide , Bonadiman Costanza , Pavese Alessandro
Published: June 2022

Municipal solid waste incineration fly ash (FA) can represent a sustainable supply of supplementary material to the construction industries if it is pre-treated to remove hazardous substances such as chloride, sulfate, and heavy metals. In this paper, the phenomenology associated with a water washing multi-cycle treatment of FA is investigated, focusing attention upon the mineral dissolution process. The efficacy of the treatment is assessed by leaching tests, according to the European Standard, and discussed in light of the occurring mineral phases. The water-to-solid (L/S) ratio is a crucial parameter, along with the number of washing cycles, for removing halite and sylvite, whereas quartz, calcite, anhydrite, and an amorphous phase remain in the solid residue. The sequential extraction method and dissolution kinetics modelling provide further elements to interpret leaching processes, and suggest that dissolution takes place through a two-step mechanism. Altogether, multi-step washing with L/S = 5 is effective in reducing contaminants under the legal limits for non-hazardous waste disposal, while the legal limits for non-reactive or reusable material cannot be completely reached, owing to sulfate and some heavy metals which still leached out from the residue.
A Novel Method for Conducting a Geoenvironmental Assessment of Undiscovered ISR-Amenable Uranium Resources: Proof-of-Concept in the Texas Coastal Plain
Authors: Gallegos Tanya J. , Stengel Victoria G. , Walton-Day Katie , Blake Johanna , Teeple Andrew , Humberson Delbert , Cahan Steven , Yager Douglas B. , Becher Kent D.
Published: June 2022

A geoenvironmental assessment methodology was developed to estimate waste quantities and disturbances that could be associated with the extraction of undiscovered uranium resources and identify areas on the landscape where uranium and other constituents of potential concern (COPCs) that may co-occur with uranium deposits in this region are likely to persist, if introduced into the environment. Prior to this work, a method was lacking to quantitively assess the environmental aspects associated with potential development of undiscovered uranium resources at a scale of a uranium resource assessment. The mining method of in situ recovery (ISR) was historically used to extract uranium from deposits in the Goliad Sand of the Texas Coastal Plain. For this reason, the study’s methodology projected the following types of wastes and disturbances commonly associated with ISR based on historical ISR mining records: the mine area, affected aquifer volume, mine pore volume, water pumped and disposed during uranium extraction and restoration, and radon emissions. Within the tract permissive for the occurrence of undiscovered uranium resources, maps and statistics of factors were derived that indicate the potential contaminant pathways. The percentage of days meeting the criteria for air stagnation indicate the potential for radon accumulation; the geochemical mobility of COPCs in groundwater in combination with effective recharge indicates the potential for infiltration of surface-derived COPCs; the geochemical mobility of COPCs in groundwater combined with hydraulic conductivity indicates the propensity for transmitting fluids away from contaminated or mined aquifers; and finally, geochemical mobility of COPCs in surface water combined with the factor for climatic erosivity (R factor) indicates the potential for COPCs to persist in surface waters due to runoff. This work resulted in a new methodology that can be applied to any undiscovered mineral resource to better understand possible wastes and disturbances associated with extraction and identify areas on the landscape where COPCs are likely to persist.
Fluorine-18 Labelled Radioligands for PET Imaging of Cyclooxygenase-2
Authors: Kaur Jatinder , Bhardwaj Atul , Wuest Frank
Published: June 2022

Molecular imaging probes enable the early and accurate detection of disease-specific biomarkers and facilitate personalized treatment of many chronic diseases, including cancer. Among current clinically used functional imaging modalities, positron emission tomography (PET) plays a significant role in cancer detection and in monitoring the response to therapeutic interventions. Several preclinical and clinical studies have demonstrated the crucial involvement of cyclooxygenase-2 (COX-2) isozyme in cancer development and progression, making COX-2 a promising cancer biomarker. A variety of COX-2-targeting PET radioligands has been developed based on anti-inflammatory drugs and selective COX-2 inhibitors. However, many of those suffer from non-specific binding and insufficient metabolic stability. This article highlights examples of COX-2-targeting PET radioligands labelled with the short-lived positron emitter 18F, including radiosynthesis and PET imaging studies published in the last decade (2012–2021).
Gold/Pentablock Terpolymer Hybrid Multifunctional Nanocarriers for Controlled Delivery of Tamoxifen: Effect of Nanostructure on Release Kinetics
Authors: Popescu Maria-Teodora , Tsitsilianis Constantinos
Published: June 2022

Here, we describe the preparation and characterization of organic/inorganic hybrid polymer multifunctional nanocarriers. Novel nanocomposites of gold nanoparticles using pH-responsive coordination pentablock terpolymers of poly(ε-caprolactone)-b-poly(ethylene oxide)-b-poly(2-vinylpyridine)-b-poly(ethylene oxide)-b-poly(ε-caprolactone), bearing or not bearing partially quaternized vinylpyridine moieties, were studied. The template morphology of the coordination pentablock terpolymer at physiological pH ranges from crew-cut to multicompartmentalized micelles which can be tuned by chemical modification of the central block. Additionally, the presence of 2VP groups allows the coordination of gold ions, which can be reduced in situ to construct gold@polymer nanohybrids. Furthermore, the possibility of tuning the gold distribution in the micelles, through partial quaternization of the central P2VP block, was also investigated. Various morphological gold colloidal nanoparticles such as gold@core-corona nanoparticles and gold@core-gold@corona nanoparticles were synthesized on the corresponding template of the pentablock terpolymer, first by coordination with gold ions, followed by reduction with NaBH4. The pentablock and gold@pentablock nanoparticles could sparingly accommodate a water-soluble drug, Tamoxifen (TAX), in their hydrophobic micellar cores. The nanostructure of the nanocarrier remarkably affects the TAX delivery kinetics. Importantly, the hybrid gold@polymer nanoparticles showed prolonged release profiles for the guest molecule, relative to the corresponding bare amphiphilic pentablock polymeric micelles. These Gold@pentablock terpolymer hybrid nanoparticles could act as a multifunctional theranostic nanoplatform, integrating sustainable pH-controlled drug delivery, diagnostic function and photothermal therapy.
Sodium-Based Cylindrical Plasmonic Waveguides in the Near-Infrared
Authors: Teng Da , Tian Yuanming , Hu Xuemei , Guan Ziyi , Gao Wencang , Li Pengyuan , Fang Hongli , Yan Jianjun , Wang Zhiwen , Wang Kai
Published: June 2022

Subwavelength optical field confinement and low-loss propagation are of great significance for compact photonic integration. However, the field confinement capability of plasmonic devices is always accompanied by the inherent Ohmic loss. Although recent studies have shown that sodium (Na) exhibits lower loss than noble metals in the near-infrared band, the field confinement ability has not been adequately assessed. Meanwhile, the high chemical reactivity of Na should be regulated for practical application. Two dielectric-coated Na nanowires, consisting of cylindrical Na nanowires with one or two dielectric layers as claddings, are proposed and investigated in this paper. Based on finite element calculations, we thoroughly study the modal fields and low-loss propagation properties of dielectric-coated Na nanowires. The results demonstrate that Na exhibits lower loss and stronger field confinement than the typical plasmonic material silver. These findings indicate the performance of plasmonic devices can be considerably improved by employing the metal Na compared with devices using noble metals, which may promote the applications in subwavelength photonic devices.
Design of a High-Efficiency Multilayer Dielectric Diffraction Grating with Enhanced Laser Damage Threshold
Authors: Cu Duy Thanh , Pham Tien Dat , Le Vu Tuan Hung , Li Meng Chi , Chen Hung Pin , Kuo Chien Cheng
Published: June 2022

Diffraction gratings are becoming increasingly widespread in optical applications, notably in lasers. This study presents the work on the characterization and evaluation of Multilayer Dielectric Diffraction Gratings (MDG) based on the finite element method using Comsol MultiPhysics software. The optimal multilayer dielectric diffraction grating structure using a rectangular three-layer structure consisting of an aluminum oxide Al2O3 layer sandwiched between two silicon dioxide SiO2 layers on a multilayer dielectric mirror is simulated. Results show that this MDG for non-polarized lasers at 1064 nm with a significantly enhanced −1st diffraction efficiency of 97.4%, reaching 98.3% for transverse-electric (TE) polarization and 96.3% for transverse-magnetic (TM) polarization. This design is also preferable in terms of the laser damage threshold (LDT) because most of the maximum electric field is spread across the high LDT material SiO2 for TE polarization and scattered outside the grating for TM polarization. This function allows the system to perform better and be more stable than normal diffraction grating under a high-intensity laser.