bluuebunny/crossref_metadata_2025 huggingface dataset:
Filtro rápido de arena para el estudio de remoción de enteroparásitos protozoos en agua
Authors: Modini Laura , Pizarro Ana , Pizarro Manuel , Zerbatto Mariel
Published: May 2018

Con el objetivo de evaluar la remoción de quistes de Giardia duodenalis y ooquistes de Cryptosporidium spp. en agua, se construyó un filtro rápido por gravedad, a escala laboratorio, con arena como material filtrante. El filtro se operó a tasa constante (200 mL/min) y nivel variable. Mediante pruebas hidráulicas se estableció el espesor del lecho (0,75 m) y el tiempo de retención (5 min). El agua para las pruebas de remoción se preparó diluyendo una suspensión de (oo)quistes con H2Od. Para recuperar los (oo)quistes no removidos, el agua filtrada se pasó a través de membranas de nitrato de celulosa de 0,45 µm. Los quistes se contaron en cámara de Neubauer mientras que para estimar la concentración de ooquistes se usó el método de Breed con coloración de Kinyoun. Se logró una remoción de 2,4 log (99,6%) de ooquistes y 1,5 log (96,9%) de quistes. Con un diseño y funcionamiento apropiado la filtración rápida puede actuar como una barrera eficaz pero no garantiza la remoción de ooquistes de Cryptosporidium y/o quistes de Giardia
Reinterpretando as plantas medicinais a partir do referencial yin-yang da Medicina Tradicional Chinesa
Authors: Portelinha Márcia Karter , Barbieri Rosa Lía , Heck Rita Maria , Alves Lima Ângela Roberta , Lopes Caroline Vasconcellos
Published: May 2018

Objetivo: interpretar o uso das plantas medicinais em uma Organização Não Governamental a partir de um dos pilares da medicina tradicional chinesa, o yin-yang. Métodos: estudo de caso de caráter qualitativo, exploratório e analítico. Aprovado pelo comitê de ética da Faculdade de Enfermagem da Universidade Federal de Pelotas, sob número 35\2012. As entrevistas semiestruturadas foram coletadas de abril a setembro de 2011. A análise foi baseada na leitura repetida da transcrição das entrevistas, buscando referências sobre as plantas medicinais que poderiam ser interpretadas nos grupos temáticos yin e yang. Resultados: os entrevistados indicaram o uso de 106 plantas medicinais, cuja interpretação a partir do pilar yin-yang permitiu identificar nove plantas correspondentes a yin/anatomia e catorze plantas correspondentes a yang/fisiologia. Considerações Finais: existem diversas formas de utilizar as potencialidades das plantas medicinais, sendo possível uma reinterpretação das plantas medicinais usadas na medicina popular brasileira sob a óptica da medicina tradicional chinesa.
Decomposing Gratitude: Representation and Integration of Cognitive Antecedents of Gratitude in the Brain
Authors: Yu Hongbo , Gao Xiaoxue , Zhou Yuanyuan , Zhou Xiaolin
Published: May 2018

Gratitude is a typical social-moral emotion that plays a crucial role in maintaining human cooperative interpersonal relationship. Although neural correlates of gratitude have been investigated, the neurocognitive processes that lead to gratitude, namely, the representation and integration of its cognitive antecedents, remain largely unknown. Here, we combined fMRI and a human social interactive task to investigate how benefactor's cost and beneficiary's benefit, two critical antecedents of gratitude, are encoded and integrated in beneficiary's brain, and how the neural processing of gratitude is converted to reciprocity. A coplayer decided whether to help a human participant (either male or female) avoid pain at his/her own monetary cost; the participants could transfer monetary points to the benefactor with the knowledge that the benefactor was unaware of this transfer. By independently manipulating monetary cost and the degree of pain reduction, we could identify the neural signatures of benefactor's cost and recipient's benefit and examine how they were integrated. Recipient's self-benefit was encoded in reward-sensitive regions (e.g., ventral striatum), whereas benefactor-cost was encoded in regions associated with mentalizing (e.g., temporoparietal junction). Gratitude was represented in perigenual anterior cingulate cortex (pgACC), the strength of which correlated with trait gratitude. Dynamic causal modeling showed that the neural signals representing benefactor-cost and self-benefit passed to pgACC via effective connectivities, suggesting an integrative role of pgACC in generating gratitude. Moreover, gyral ACC plays an intermediary role in converting gratitude representation into reciprocal behaviors. Our findings provide a neural mechanistic account of gratitude and its role in social-moral life.

SIGNIFICANCE STATEMENTGratitude plays an integral role in subjective well-being and harmonious interpersonal relationships. However, the neurocognitive processes through which various components and antecedents of gratitude are integrated remain largely unknown. We developed a new interpersonal paradigm to independently and parametrically manipulate two antecedents of gratitude in a helping context, namely, the benefit to beneficiary and the cost to benefactor, to examine their representation and integration in the beneficiary's brain using fMRI. We found the neural encoding of self-benefit and benefactor-cost in reward- and mentalizing-related brain areas, respectively. More importantly, by examining effective connectivity, we showed that these componential signals are passed to perigenual anterior cingulate cortex, which tracks trial-by-trial gratitude levels. Our study thus provides a neural mechanistic account of gratitude.
Defects in the Neuroendocrine Axis Contribute to Global Development Delay in a Drosophila Model of NGLY1 Deficiency
Authors: Rodriguez Tamy Portillo , Mast Joshua D , Hartl Tom , Lee Tom , Sand Peter , Perlstein Ethan O
Published: May 2018

N-glycanase 1 (NGLY1) Deficiency is a rare monogenic multi-system disorder first described in 2014. NGLY1 is evolutionarily conserved in model organisms. Here we conducted a natural history study and chemical-modifier screen on the Drosophila melanogaster NGLY1 homolog, Pngl. We generated a new fly model of NGLY1 Deficiency, engineered with a nonsense mutation in Pngl at codon 420 that results in a truncation of the C-terminal carbohydrate-binding PAW domain. Homozygous mutant animals exhibit global development delay, pupal lethality and small body size as adults. We developed a 96-well-plate, image-based, quantitative assay of Drosophila larval size for use in a screen of the 2,560-member Microsource Spectrum compound library of FDA approved drugs, bioactive tool compounds, and natural products. We found that the cholesterol-derived ecdysteroid molting hormone 20-hydroxyecdysone (20E) partially rescued the global developmental delay in mutant homozygotes. Targeted expression of a human NGLY1 transgene to tissues involved in ecdysteroidogenesis, e.g., prothoracic gland, also partially rescues global developmental delay in mutant homozygotes. Finally, the proteasome inhibitor bortezomib is a potent enhancer of global developmental delay in our fly model, evidence of a defective proteasome “bounce-back” response that is also observed in nematode and cellular models of NGLY1 Deficiency. Together, these results demonstrate the therapeutic relevance of a new fly model of NGLY1 Deficiency for drug discovery and gene modifier screens.
Calcineurin Regulatory Subunit Calcium-Binding Domains Differentially Contribute to Calcineurin Signaling in Saccharomyces cerevisiae
Authors: Connolly Sean , Quasi-Woode Devona , Waldron Laura , Eberly Christian , Waters Kerri , Muller Eric M , Kingsbury Tami J
Published: May 2018

The protein phosphatase calcineurin is central to Ca2+ signaling pathways from yeast to humans. Full activation of calcineurin requires Ca2+ binding to the regulatory subunit CNB, comprised of four Ca2+-binding EF hand domains, and recruitment of Ca2+-calmodulin. Here we report the consequences of disrupting Ca2+ binding to individual Cnb1 EF hand domains on calcineurin function in Saccharomyces cerevisiae. Calcineurin activity was monitored via quantitation of the calcineurin-dependent reporter gene, CDRE-lacZ, and calcineurin-dependent growth under conditions of environmental stress. Mutation of EF2 dramatically reduced CDRE-lacZ expression and failed to support calcineurin-dependent growth. In contrast, Ca2+ binding to EF4 was largely dispensable for calcineurin function. Mutation of EF1 and EF3 exerted intermediate phenotypes. Reduced activity of EF1, EF2, or EF3 mutant calcineurin was also observed in yeast lacking functional calmodulin and could not be rescued by expression of a truncated catalytic subunit lacking the C-terminal autoinhibitory domain either alone or in conjunction with the calmodulin binding and autoinhibitory segment domains. Ca2+ binding to EF1, EF2, and EF3 in response to intracellular Ca2+ signals therefore has functions in phosphatase activation beyond calmodulin recruitment and displacement of known autoinhibitory domains. Disruption of Ca2+ binding to EF1, EF2, or EF3 reduced Ca2+ responsiveness of calcineurin, but increased the sensitivity of calcineurin to immunophilin-immunosuppressant inhibition. Mutation of EF2 also increased the susceptibility of calcineurin to hydrogen peroxide inactivation. Our observations indicate that distinct Cnb1 EF hand domains differentially affect calcineurin function in vivo, and that EF4 is not essential despite conservation across taxa.
Management of Type 1 Diabetes With a Very Low–Carbohydrate Diet
Authors: Lennerz Belinda S. , Barton Anna , Bernstein Richard K. , Dikeman R. David , Diulus Carrie , Hallberg Sarah , Rhodes Erinn T. , Ebbeling Cara B. , Westman Eric C. , Yancy William S. , Ludwig David S.
Published: May 2018

OBJECTIVES:

To evaluate glycemic control among children and adults with type 1 diabetes mellitus (T1DM) who consume a very low–carbohydrate diet (VLCD).

METHODS:

We conducted an online survey of an international social media group for people with T1DM who follow a VLCD. Respondents included adults and parents of children with T1DM. We assessed current hemoglobin A1c (HbA1c) (primary measure), change in HbA1c after the self-reported beginning of the VLCD, total daily insulin dose, and adverse events. We obtained confirmatory data from diabetes care providers and medical records.

RESULTS:

Of 316 respondents, 131 (42%) were parents of children with T1DM, and 57% were of female sex. Suggestive evidence of T1DM (based on a 3-tier scoring system in which researchers took into consideration age and weight at diagnosis, pancreatic autoimmunity, insulin requirement, and clinical presentation) was obtained for 273 (86%) respondents. The mean age at diagnosis was 16 ± 14 years, the duration of diabetes was 11 ± 13 years, and the time following a VLCD was 2.2 ± 3.9 years. Participants had a mean daily carbohydrate intake of 36 ± 15 g. Reported mean HbA1c was 5.67% ± 0.66%. Only 7 (2%) respondents reported diabetes-related hospitalizations in the past year, including 4 (1%) for ketoacidosis and 2 (1%) for hypoglycemia.

CONCLUSIONS:

Exceptional glycemic control of T1DM with low rates of adverse events was reported by a community of children and adults who consume a VLCD. The generalizability of these findings requires further studies, including high-quality randomized controlled trials.
Cosmolaelaps keni a polyphagous predatory mite on various insect and mite species (Acari: Laelapidae)
Authors: Momen F. M. , Metwally A. S. , Nasr A. K. , Gesraha M. , Mahmoud Y. A. , Saleh K. H. M.
Published: May 2018

The predatory mite Cosmolaelaps keni is a native laelapid mite in Egypt. Development and adult longevity durations as well as fecundity of C. keni reared on five types of prey, Bactrocera zonata, Spodoptera littoralis and Corcyra cephalonica eggs (as insects prey), Aceria dioscoridis and Caloglyphus rodriguez (as mites prey) were determined under laboratory conditions. Cosmolaelaps keni was able to feed, develop and sustain oviposition on all examined insect and mite species. The mean developmental period of C. keni on B. zonata and S. littoralis eggs was significantly shorter than those fed C. cephalonica eggs and both mite species. Deutonymphs of C. keni were consumed similar number of insect eggs, while during the oviposition period, more B. zonata eggs were consumed. A diet of C. rodriguez provided the shortest oviposition period and longevity, while C. cephalonica eggs showed the longest period in this respect. During the oviposition period, preying on B. zonata and C. cephalonica eggs gave the highest fecundity rates for the predatory mite compared to S. littoralis and C. rodriguez. The sex ratio of C. keni progeny was in female-biased. Bactrocera zonata eggs as a prey gave the highest rate of oviposition as shown by the maximum fertility, net reproductive rate and gross reproductive rate as well as the shortest generation time compared to other tested prey; therefore B. zonata eggs were appropriate factitious prey for oviposition and mass rearing of the predatory mite C. keni.
Development and validation of stability-indicating RP-HPLC method for the simultaneous determination of tizanidine HCL and meloxicam in rabbit's plasma
Authors: Zaman Muhammad , Hanif Muhammad , Khan Najm Ul Hassan , Mahmood Asif , Qaisar Muhammad Naeem , Ali Huma
Published: May 2018

High-performance liquid chromatography (HPLC) is a widely used technique for the simultaneous detection and quantification of different drugs. The purpose of the current study was to develop a simple and cost-effective reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of tizanidine (TZN) HCl and meloxicam (MLX) in rabbit's plasma. Assay of TZN and MLX was performed after extraction of drug from plasma by liquid–liquid extraction technique using methanol and diethyl ether as protein precipitants. Isocratic elution was performed in a Kromasil® C18 column (dimension, 250 × 4.60 mm; particle size, 5 μm) with mobile phase consisting of methanol–water (8:2). Orthophosphoric acid was used to adjust the pH of the mobile phase 3.0, and detection was done at 228 nm. Flow rate was 0.8 mL/min with ambient temperature and average operating pressure of 1400 psig. Retention time of TZN was 2.612 min and that of MLX was 6.960 min with a resolution of 3.18. Both drugs showed satisfactory linearity in the range of 10 to 50 ng/mL with correlation coefficients (R2) of 0.9989 and 0.9972 for TZN and MLX, respectively. The developed method was validated successfully for linearity, system suitability, intra-day and inter-day accuracy, and precision, robustness, and specificity following International Conference on Harmonization (ICH) guidelines. Conclusively, a precise, stable, reproducible, economical, and suitable method for estimation of pharmacokinetic evaluation was developed and validated.
Study on the drug–drug interaction of antituberculosis drugs — PA-824 and moxifloxacin based on pharmacokinetics in rats by LC–MS/MS
Authors: Jing Juan , Jia Yuting , Feng Tian , Xie Tian , Li Zhoupeng , Hao Yong , Wang Libin
Published: May 2018

A simple, rapid, and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for the simultaneous quantitation of PA-824 and moxifloxacin in rat plasma using carbamazepine as an internal standard (IS). The sample preparation involved a one-step protein precipitation method with methanol. The separation was performed on Inertsil® ODS3 C18 column (150 mm × 4.6 mm, 5 μm) and maintained at 30 °C. The mobile phase consisted of 0.1% formic acid in acetonitrile–water (90:10 v/v) with fast isocratic elution at a flow rate of 0.6 mL/min and a run time of 10 min. A mass spectrometer was run in the positive ion electrospray ionization (ESI) mode using multiple reaction monitoring (MRM) to monitor the mass transitions. The MRM transitions were chosen to be m/z 360.1 → m/z 175.0 for PA-824, m/z 402.0 → m/z 383.9 for moxifloxacin, and m/z 237.1 → m/z 194.0 for IS. The method was fully validated in terms of selectivity, linearity, accuracy, precision, matrix effect, recovery, and stability, respectively. The method was successfully applied to drug–drug interaction (DDI) study of PA-824 and moxifloxacin in rats. The results show that the main pharmacokinetic parameters of PA-824, namely, Tmax, t1/2, and AUC(0–t), increased more in the PA-824 and moxifloxacin group than in the PA-824 group. However, there were little changes in the main pharmacokinetic parameters of moxifloxacin from single and combined groups.
Mahasiswa tingkat kepaniteraan Universitas Hasanuddin Makassar, Indonesia
Authors: Rieuwpassa Irene Edith , Rahmat Rahmat , Karlina Karlina
Published: May 2018

The aim of this study was to determine the effectiveness of Aloe vera extract in inhibiting the growth ofStaphylococcus aureus and to measure the minimum inhibitory concentration (MIC) on the growth of the bacteria.The method using the manufacture of aloe extract, extract dilution, purified Staphylococcus aureus, MIC test andview the zone of inhibition. The results show that the (MIC) of Aloe extract was at concentration of 0.25. After astatistical test with ANOVA test on survey data (α<0.05), then the result obtained 0.000. This shows significantdifferences between various concentrations of Aloe vera extract in inhibiting the growth of Staphylococcus aureusbacteria. The higher the concentrationof Aloe vera extract, the more expand diameter of inhibition zone. Thusextracts of Aloe vera may be used to inhibit the growth of Staphylococcus aureus.
Penatalaksanaan impaksi gigi kaninus dengan cara kombinasi pembedahan dan ortodontik Management of impacted canine with combination of surgery and orthodontic
Authors: Oscar Franky
Published: May 2018

Complete tooth eruption, especially canine, will affect facial esthetic and the harmony. Surgical procedure will helpto expose an impacted tooth determining orthodontically into dental arch. Clinical and radiographic examinationshould be taken for locating the position of the tooth. The flap was designed differently between labially or palatallyimpacted canine. A 16 years old female came to clinic with Class III Angle dental and skeletal malocclusion with animpacted of upper left canine. She was treated with fixed orthodontic appliance using edgewise technique andsurgical exposure using closed-eruption technique. The proper diagnosis and management between oral andmaxillofacial surgery, and orthodontics will guarantee good result.
Bleaching internal untuk merawat perubahan warna gigi insisivus sentralis kanan atas (Laporan Kasus)Internal bleaching as a treatment to color-changed of right upper centralis canine (case report)
Authors: Dianty Firsta , Sukartini Endang , Armilia Milly
Published: May 2018

Intracoronal bleeding in the tooth pulp due to trauma is one of the causes of tooth discoloration. Anterior toothdiscoloration often cause esthetic problem that affect individual perfomance. Internal bleaching is a non-vitalbleaching method performed after endodontic treatment using strong oxidator agent in pulp chamber. This casereport discusses about internal bleaching treatment with walking bleach technique on tooth 11 after endodontictreatment. Concentration of hidrogen perokxide 35% was applied for 3-7 days, this procedure was repeated untilthe tooth color was gained. The tooth color was accomplished in second visit. As conclusion is internal bleaching astreatment of tooth discoloration due to trauma can provide a good result with good endodontic treatment.
Status kebersihan gigi dan mulut Suku Papua pengunyah pinang di Manado Oral and dental hygiene status of Papua’s Areca nut chewer
Authors: Siagian Krista Veronica
Published: May 2018

Areca nut chewing habits is one of the greatest threats to oral health today. It is estimated that 600 million peoplehave areca nut chewing habit, including Papua ethnic group. The purpose of this study was to evaluate the oralhygiene status of Papua ethnic group who have areca nut chewing habits living in at Manado. The study is adescriptive cross sectional type using survey technique. Thirty respondents, males and females aged between 18 to40 years old were collected as purposive sample. Clinical data of oral hygiene status was assesed by OHI-S IndexGreene and Vermillion, including debris and calculus score. Results indicated that oral hygiene score (OHI-S)is2.32. The conclusion of this study is the oral hygiene status of Papua’s areca nut chewers living in at Manado, ismedium category.
Diagnosis dan tatalaksana nyeri pada rongga mulut yang disebabkan oleh infeksi virus herpes Diagnosis and management of pain in oral cavity caused by herpes virus infection
Authors: Marlina Erni , Yusran Ali , Nazaruddin Zohra
Published: May 2018

There are 80 types of known herpes virus, 8 of them can cause infection on humans. They are herpes simplex virus(HSV) 1 and 2, varicella zoster virus (VZV), cytomegalovirus, Epstein-Barr virus, human herpes virus (HHV6) Aand B, and paramyxovirus. HSV1, HSV2, and VZV are the virus known to cause oral mucosal diseases. This paperaims to review and discuss orofacial pain caused by herpes virus infection. Detail anamnesis about prodromal signand symptom with clinical features that vesicles, labial and intraoral lesions, and unilateral distribution of lesionsare characterized oral herpes virus infections. It can be concluded that detailed anamnesis and an understandingabout oral clinical sign and symptom may confirm diagnosis of herpes virus infections.
Upaya pencegahan yang dilakukan oleh dokter gigi di Makassar terhadap bahaya akibat penggunaan amalgam Prevention of dentists in Makassar to the risk of amalgam
Authors: Edy Sarwo , Samad Rasmidar
Published: May 2018

Makassar is a big city with the highest number of dentists in South Sulawesi. Currently there are many dentists in thecity of Makassar using amalgam as a filling material for cheaper and more durable, so it is preferred by patients.Generally, they have ignored the dangers of exposure to mercury in amalgam through breathing or skin contact. For itwas on this descriptive observational study noted the efforts of Makassar dentist to prevent hazards due to the use ofamalgam in practice. From the general population of dentists who were registered as members of the IndonesianDentists Association (IDA) branch Makassar as much as 258 people, based on the opinions of Gay and Diehl withanticipation drop out of 20%, was obtained a sample of 100 people, but only 67 respondents (male = 13, female = 54)uses amalgam. The results are efforts to prevent the danger of amalgam achieved 51-75% by 32 respondents (47.7%),76-100% by 25 respondents (37.3%), 26-50% by 10 respondents (15%), and no respondents who seek prevention ofcross infection hazard to the achievement of 0-25%. Efforts to prevent with the achievement of 51-75% (32respondents) and 76-100% (25 respondents), could be said to have met the standard, concluded that 85% of dentists inMakassar have good enough prevention efforts to amalgam while doing their practice.
Pengaruh pemakaian bahan restorasi mahkota terhadap kesehatan jaringanperiodontal Effect of using crown restoration material to the health of periodontal tissue
Authors: Machmud Edy , Herawati Herawati
Published: May 2018

Using crown as restoration to replace missing natural teeth is currently very popular. Successful restoration is therestoration that can last in a long time in the oral cavity. But sometimes there is a result of the use of restorativematerials that can’t be accepted by the body tissues. Therefore this study with a quasi experimental longitudinal designwith samples of 100 people user crown (porcelain, acrylic and metal crown). Prior using denture, the patient is given adental health education (DHE) and tartar cleaning and maintenance agreement. CPITN examination for signs ofinflammation (gingival discoloration, gingival bleeding, calculus and pocket depth) before and after the use of crown3, 6 and 12 months. Data were collected and analyzed by independent t-test. The results showed that there weresignificant differences in the results at month 3 to month 6, and month 6 to month 12 in acrylic and metal restoration(P <0.05). While the porcelain restoration is no significant difference in outcome between treatments at months 3, 6and 12 (P> 0.05). It was concluded that the porcelain restoration is a material that can be accepted by the body because it does not cause gingival inflammation.
Reimplantasi gigi avulsi Reimplantation of avulsed teeth
Authors: Dahong Fonny , Winarso Lies Wahyuni
Published: May 2018

It is well known dental reimplantation is indicated following traumatic avulsion by the preservation of vitality in theperiodontal ligament with condition asepsis. Reimplantation refers to the insertion and temporary of completely orpartially avulsed teeth that have resulted from traumatic injury. Dental reimplantation of an avulsed tooth dependson certain clinical conditions like physiological status of periodontal ligament, the stage of root development andthe length of extra oral time. Depending on the patient’s age, retention of the permanent incisor can maintain theaesthetic appearance, occlusal function and alveolar ridge height. Though the risk of progressive replacementresorption and subsequent tooth loss is high after a long dry storage. This article presents management of one casewith avulsed permanent incisors that were storage in dry condition for seventeen hours
Restorasi mahkota pasak pada gigi dengan jarak serviko-oklusal pendek Dowel crown restoration on tooth with short cervico-occlusal distance
Authors: Bonifacius Setyawan , Rais Sri Wahyuningsih
Published: May 2018

Dowel crown restoration is a restoration which is often performed by dentists in daily practice. Dental crowns requirethe dowel crown restoration due to several factors including the crown damage cause has been very severe. Dowelcrown restoration usually consists of two parts, namely dowel with core and crown restoration on it. The core must behigh enough in cervico-occlusal direction to obtain sufficient retention for the attachment of the crown. In some casesthis condition cannot be fulfilled because the cervico-occlusal distances are too short resulting from extrusion of thetooth. This paper is reporting a case of management tooth maxillary second premolar with limited cervico-occlusaldimension with dowel crown restoration in one unit. The technique was a combination of direct and indirect method.The result was an accurate and retentive dowel crown, and has a good aesthetic.
Penggunaan mahkota dari gigi alami yang fraktur servikal sebagai pontik Using crown of natural tooth as a pontic on a tooth with cervical fracture
Authors: Syam Syamsiah
Published: May 2018

Replacement of missing anterior teeth can cause many problems, especially aesthetic. Immediate replacement of theanterior teeth can prevent psychological and social disruption to patients. This article explain the use of natural toothcrowns as a pontic following a cervical fracture using fiber reinforced material as post and to bond the natural toothcrown with adjacent teeth. The use of a natural tooth crown result excellent esthetic, efficient, and the procedure ismore economic
Evaluasi kesesuaian standar ukuran vertikal slot breket ortodonti The evaluation of the standard validity of vertical orthodontic bracket slot size
Authors: Silitonga Parta L. , Thahar Bergman , Mardiati Endah , Hambali Tono , Soemantri Eky Soeria
Published: May 2018

On orthodontic treatment, accuracy of orthodontic brackets slot size is essential for maximum interaction between thewire and brackets, so as to distribute the optimal force for tooth movement. The purpose of this study was to determinethe accuracy of orthodontic brackets slot size to 0.018 inch standard sizes that are available in the market. The samplewas selected by using a random sampling system, which consists of 15 types of brackets of 8 bracket manufacturerswith a total sample of 300 samples. Measurements performed on 4 sides of slot bracket, namely the outer and innersides of the brackets slot, on mesial and distal sides, using a profile projector (Mitutoyo Type PJ250). The results werestatistically tested using t-test one side. All brackets have a slot size significantly larger than the standard size. Theaverage percentage of magnification ranged from 5.18-24.29%. So, it was concluded that the size of the slot bracketsavailable in the Indonesian market greater than their standard.
Aktivitas antibakteri ekstrak terstandar akar sidaguri (S.rhombifolia) terhadap E. faecalis dan Actinomyces spp. Antibacterial activity of standardized extract of sidaguri root (S.rhombifolia) against E. faecalis and Actinomyces spp.
Authors: Tanumihardja Maria , Darmayana Darmayana , Natsir Nurhayati , Mattulada Indrya K.
Published: May 2018

According to 2009 Indonesian Health Profile Data, pulp and periapical diseases were 8thof the 10 outpatients athospital. This situation tends to increase become the big of 7. Pulp and periapical diseases occur due to bacterialinfection in the pulp tissue. The bacteria are often found in the root canal, namely E. faecalis, and Actinomyces spp.Various herbs commonly used by people to treat dental diseases, i.e. root sidaguri. However, sidaguri plants have notbeen studied in the field of dentistry as an antibacterial. This study aimed to determine the effects of root sidaguriagainst E. faecalis and Actinomyces spp. Inhibition test of ethanol extract of the roots sidaguri against E. faecalis andActinomyces spp. using agar diffusion method at concentrations of 5%, 10%, 15%, and 20% based on themeasurement of inhibition zone by each concentration. The greatest inhibition against E. faecalis was performed by concentration of 20% (p<0.05), while no inhibition zone was found on Actinomyces spp. It was concluded that theethanol extract of the sidaguri roots was the most effective against E. faecalis at concentration of 20%, but not effectiveat all against Actinomyces spp.
Jumlah sel makrofag gingiva tikus wistar jantan yang diinduksi periodontitis setelah pemberian ekstrak etanolik kulit manggis Amount of machropahges cells in periodontitis-induced wistar rats after mangosteen rind etanolic extract administration
Authors: Prasetya Rendra Chriestedy
Published: May 2018

Periodontitis is characterized with macrophages infiltration that is used to fagocyt bacteria which infiltrated atgingiva. Mangosteen rind contains xantone, an anti-inflammatory substance which inhibits the synthesis of PGE2 andtherefore reduce inflammatory cells infiltration. This research aimed to study machropahges cells infiltration inperiodontitis-induced wistar rats after administration of mangosteen rind etanolic extract. Forty eight male wistar ratswere induced periodontitis for 7 days. After the ligation was taken out, the rats were divided into 4 groups, and treatedorally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen and saline. The rats were sacrified on the 1st, 3rd, 4th, and 7th day after the treatment. Their anterior lower jaws were processed histologically and stained withhematoxylin eosin. Two-way anova test showed a significant difference among groups (p<0.05). It was concluded thatmangosteen rind etanolic extract reduced the number of macropaghes cells gingiva in periodontitis male wistar rats.
Aktivitas antibakteri ekstrak metanol batang pisang mauli (Musa sp)terhadap Streptococcus mutans Antibacterial activity of methanol extract of mauli banana (Musa sp)stems against Streptococcus mutans
Authors: Apriasari Maharani Laillyza , Fadhilah Ahmad , Carabelly Amy N.
Published: May 2018

There are many traditional medicines that can be used as alternative medicine. One of them is methanol extract ofmauli banana stems. Some people in North of Hulu Sungai Banjarmasin use it as healing agent. The aim of thisresearch was to test antibacterial activity of mauli banana stems extract against Streptococcus mutans; as well as todetermine the effective concentration of its antibacterial activity against S.mutans. The extraction was conducted bymaceration method. Viscous extracts were diluted by using 10% dimethyl sulfoxide and filtered with Whatman paperfilter. Those extracts were divided into four treatment groups: 6.25% (I), 12.5% (II), 25% (III), and control (-)(IV).Groups I, II, and III were added with Muller Hinton media to reach the concentrations. 100 μL S.mutans suspension of0.5 Mc Farland was administered to each concentration, incubated for 18 hours, diluted to 1/10 using NaCl. 100 μLwas taken and planted to blood agar media for 24 hours incubation. The numbers of S.mutans colonies were countedand statistically analysed by one-way anova test and post hoc LSD (p<0.05). The results showed that there weresignificant differences between each treatment, and extract concentration of 25% had better antibacterial activity thanthe others. It was concluded that extract of mauli banana steams have antibacterial activity against S.mutans, andmauli banana stem have the potential to be used as an alternative herbal mouthwash.
Bentuk preparasi akhiran servikal mahkota porcelain fused to metal mempengaruhi insidensi gingivitis pada gigi insisivus sentralis rahang atas Preparation shape of cervical edge of porcelain fused to metal crown affect incidence of gingivitis at maxillary central incisive teeth
Authors: Karim Abd. , Dharmautama Moh. , Machmud Edy
Published: May 2018

The selection of cervical edge form of porcelain fused to metal (PFM)crown is very important because it is associatedwith the incidence of gingivitis. Improper types of cervical edge may cause a gap at the edge; which is a good place forthe growth and development of dental plaque bacteria that play a major role in the occurrence of inflammation. Thisstudy aims to determine the effect of the cervical edge preparation shape of PFM crown against bacterial plaqueadhesion and tissue inflammation supporting abutments, as well as to determine the best shape of the cervical edgepreparation for PFM crown restoration. This Clinical experimental research with time series design is performed to 3groups of cervical edge preparation design, i.e. shoulder, bevel shoulder and deep chamfer. Sample of 15 peopledivided equally into the 3 groups. Examination of the clinical signs of gingivitis (gingival discoloration, bleeding onprobing, and pocket depth) and the number of bacterial colonies were performed before tooth preparation; 1, 7, and 21days after insertion. The data were processed statistically by ANOVA. The results showed the form shoulder bevel edgegives the most noticeable impact to gingivitis than other types.
SP-C1 tongue squamous cell carcinoma, ethanol fraction of ant nest plant, proliferation
Authors: Achmad Harun , Supriatno Supriatno , Marhamah Marhamah , Rasmidar Rasmidar
Published: May 2018

Squamous cell carcinoma at the tongue is a malignant tumor derived from epithelial mucosa of the oral cavity. Thisstudy aims to identify and to analyze the effect of flavonoid fraction of etanol of ant nests plants (Myrmecodia pendans)as an anti-cancer barrier against the proliferation of SP-C1 tongue cancer cells. The study was conducted with pureexperimental laboratory methods using Supri's-Clone 1 (SP-C1) human tongue cancer cells cultured. Researchgradually begins with determination, extraction and fractionation ant nests plant, cytotoxicity test to get a fraction offlavonoids that have anticancer potential,and finaly testing the proliferation barriers. Cytotoxicity test showed thatfrom the highest concentration (1000 μg/mL) to the lowest concentration (7.8125 μg/mL) in etanol fraction, fractionhexan and water caused Sp-C1 human tongue cancer cells death significantly. Ethanol fraction LCvalues of 938,003.These results were obtained from the linear equivalent of the relation between log consentration and probit. Flavonoidetanol fraction of ant nest has an inhibition effect against proliferation of SP-C1. Antiproliferative analysis of flavonoidfraction of ethanol flavonoid based on concentration and incubation time on optical density absorbation SP-C1 cellswas statistically highly significant (p=0.00).
Efektivitas ekstrak metanol batang pisang mauli 100%padapenyembuhan luka punggung mencit (Mus musculus)ditinjau dari jumlah sel radang (Effectiveness of 100%extract of Mauli Banana stem to healing process of mice’s (Mus musculus)back from the perspective of the number of inflamed cell)
Authors: Apriasari Maharani Laillyza , Carabelly Amy Nindia , Aprilia Gusti Febby
Published: May 2018

Banana plant especially its trunk contains many kinds of fitokimia such as saponin, flavonoid, and tannin whichfunction as antibiotic and fasten the healing process. Mauli banana stem extract is often used by the people in HuluSungai Utara Banjarmasin Province to fasten healing skin injury. The purpose of this research is to histopathologicallyexamine the effectiveness of Mauli Banana stem extract to the healing process of the mice’s back (Mus musculus)onthe 3rd day. This purely true experimental research with post test only with control design used 27 mices which weredivided to 3 groups. Treatment group was given methanol extract of mauli banana stem whose average scores ofneutrofil and macrofag are representatively 2.333 and 1; negative control group was given aquadest with its averageneutrofil and macrofag scores are representatively 1.055 dan 1.678; and positive control group was given standardmedicine which contained Aloe veraextract with its average neutrofil and macrofag scores are representatively 1.066dan 1.667. The result of Kruskall-Wallis and Mann-Whiney showed a significant difference between treatment groupand negative control group. It can be concluded that the extract methanol of mauli banana stem on the healing processof injury to mancit’s back histopathologically influences the decreasing acute inflamed (neutrofil) cell and theincreasing chronic inflamed (macrophage) cell on the third day. In this case the extract of mauli banana stem ismedically potential to fasten the healing process of injury.
Management of allergic stomatitis due to dailyfoodconsumption(Penatalaksanaan stomatitis alergika akibat konsumsi makanan sehari-hari)
Authors: Indrawati Erni , Harijanti Kus
Published: May 2018

Allergic stomatitis or stomatitis venenata is a hypersensitivity reaction caused by an allergen from medicines, foods,dental materials (restoration material, prosthetic, orthodontic appliance, mercury, acrylic, cobalt). This report willdiscusses the management of food allergic stomatitis. The common cause of food allergy in adult patients are orange(citrus fruit), nuts, fish, marine fish and wheat. Oral manifestations of allergic stomatitis begin with multiple vesiclesand becomes fibrin-covered ulcers with erythematous edges accompanied by inflammation and pain. The patient was a53-year-old woman who has suffered stomatitis since 6 months ago, and had seen dentist in private practice andgovernment dental-practice (hospitals and health centers). The patient has an a allergy history. Intra oral examinationfound the presence of multiple ulcers on the buccal mucosa upper right, cheek mucosa lower right and tongue. Thefinal diagnosis of the case is allergic stomatitis due to daily food alergic base on anamesis, clinical examination andlaboratory tests. Treatment to prevent allergic stomatitis causes, symptomatic therapy, and topical corticosteroids. Itwas concluded that management of allergic stomatitis should avoid the food as primary causes, giving mouthwashcontaining analgesic/anti-inflammatory and topical corticosteroids.
Penanganan herpes simpleks labialis rekuren (Management of recurrent herpes simplex labialis)
Authors: Harlina Harlina , Marlina Erni , Athifah Athifah
Published: May 2018

Infections of Herpes simplex virus 1 (HSV-1), is a viral infection that is often in the form of primary gingivostomatitis.Its mild form usually provides subclinical symptoms that are not clear. Herpes virus transmission can occur throughcontact with secretions from the mouth mucocutaneous and patient’s genital. It is reported that a 10 years-old malepatient complaining of pain in the left corner of the lip and lower lip mucosa experienced since three days ago. Sevendays ago there was a similar injury in the right corner of his mouth. This condition was often experienced the patient.As its management of HSV-1, the patient was given acyclovir, paracetamol and vitamin B complex. After 7 days thepatient was declared healed. It is concluded that treatment for HSV-1 infected patients include causative treatmenttogether with analgesic and antipyretic. Patients are also prouded with supporting treatments, such as high-caloriand-proteindilutedfood,multivitamins,andanastheticummouthwasher.
Lama perendaman gigi di dalam air perasan jeruk nipis (Citrus aurantifolia Swingle) mempengaruhi kedalaman porositas mikro email (Duration of immersing teeth in lime (Citrus aurantifolia Swingle) juice affects on microporosity depth of enamel)
Authors: Hediana Vanda Ayu Kartika , Probosari Niken , Setyorini Dyah
Published: May 2018

Lime juice contains some organic acids. It is a plant that is widely consumed by people.Organic acid content causes the lime has an acidic pH. The consumption of beverages containing excess acid may cause tooth erosion due to demineralizationonenamel surface.Demineralization happens constantly causes the loss of some enamel prismata and formamicroporosityinthe enamel. This research wasconducted to measure the depth of enamel microporosity of the teeth enamel is soaked in limejuice. Samples were maxillary first premolar teeth which were divided into two groups: control (soaked in artificial saliva)and treatment (soaked in lime juice) respectively for 5 and 10 minutes. Depth measurement of enamel microporosity wasmeasuredby using a scanning electron microscope, which showed no significant difference (α<0.05) between the depth ofthe microporosity of the enamel were soaked lemon juice for 5 minutes and 10 minutes. It was concluded that the longer thetooth enamel soaking in lime juice causes deeper microporosity.
Kualitas hidup manula yang menggunakan gigi tiruan lengkap berdasarkan OHIP -14 di Kota Makassar (Quality of life of elderly using complete denture based on OHIP-14 in Makassar)
Authors: Berutu Muzdalifah Solina , Dharmautama Moh.
Published: May 2018

Complete dentures are not always functioning well in replacing teeth. Mastication, esthetics and phonetic has beenknown to affect the quality of life. This study aims to determine the quality of life of elderly who use the full denture.Descriptive analytic research with cross sectional study design was conducted in Makassar on May-June 2014. Thesample was obtained by stratified random sampling, by conducting interviews based on OHIP-14 questionnaires, doorto door on 162 elderly respondents. The results show that the highest score of the seven dimensions of OHIP-14 is on thedimensions of physical disability, and the lower the score obtained, the better the quality of life. This research obtaineda mean score of 50.58, so it was concluded that the quality of life of the elderly in Makassar which uses the full denturewas categorized as good.
Penilaiankebersihansepertigaapikaldindingsaluranakardarismearlayer dengan menggunakan rotary instrument dengan disain convex triangular dan rectangular cross section (Hygiene assessment on apical third of root canal wall of smear layer using rotary instrument design convex triangular and rectangular cross section)
Authors: Amda Nurni , N Juni Jekti , Trilaksana Aries Chandra , Rovani Christine Anastasia , Natsir Nurhayaty , Mattulada Indrya Kirana
Published: May 2018

This study aim to compare the smear layer clearance on the apical third of the root canal walls instrumented withconvex triangular cross- section designed hand instruments, rectangular cross-section and convex triangular crosssectiondesigned rotary instruments. Mandibular premolars that met the inclusion criteria were divided into 3 groupsof 10 samples, and prepared using convex triangular cross section design hand instruments, convex triangular crosssectionandrectangularcross-section design rotary instruments. After preparation and drainage, the sample was cutinto halves using a stainless steel chisel, a section was randomly selected and examined using SEM with 1000 X. Thisstudy showed that the smear layer clearance of hand instrument group was the lowest compared to other two groups, the rotary instruments with rectangular cross-section design showed the highest percentage smear layer clearanceamong the three groups.It was concluded that root canal preparation using a rotary instrument design convextriangular cross section produces hygiene apical third of the canal walls of the smear layer significantly compared tomanual instruments designed convex triangular cross section and root canal preparation with a rotary instrument designof rectangular cross section produces hygiene apical third of the wall root canal of the smear layer significantlycompared convex design of triangular cross section.
Efek samping terapi radiasi penderita kanker kepala dan leher pada kelenjar saliva
Authors: Yunus Barunawaty
Published: May 2018

Nasopharyngeal cancer has different malignancy types, based on its location. Themost frequent type of nasopharyngeal cancer is carcinoma of squamous cell whichhappens on cells inside nose, mouth, and throat. The rare types happened such assalivary gland tumor, lymphoma, and sarcoma. There are three main therapies totreat nasopharyngeal cancer; they are radiation therapy, surgery, and chemotherapy.The main treatment is radiation therapy or surgery and chemotherapy or combinationboth of them. Chemotherapy is often conducted as an additional treatment.Combination treatment between those three treatments optimally can be used fornasopharyngeal cancer patient based on the location and disease stadium. Radiationtherapy on nasopharyngeal cancer can caused some side effects, such as mucositis,salivary gland dysfunction, taste sense dysfunction and malnutrition, tooth disorders,bone transforming, cutaneous transforming, nerve disorders, decreasing ofintellectual, lost of hearing sense, complication of malignant cancer caused byradiation, and intracranial bleeding.
Perbandingan sitotoksisitas Endomethasone, AH Plus, dan Apexit Plus terhadap sel fibroblas dengan teknik root dipping
Authors: Rovani Christine A. , Kamizar Kamizar , Usman Munyati
Published: May 2018

The purpose of this study was to evaluate the lowest cytotoxicity of three endodonticsealers, Apexit Plus, AH Plus and Endomethasone that can be used safely. The crownof 30 intact lower premolars were removed at cementodentinal juntion and the teethwere prepared with crown down technique with # 20 initial file and # 30 masterapical file. After sterilization, the 30 teeth were randomly divided into three groupsand filled with gutta percha using laterally condensation technique and Apexit Plus,AH Plus, and Endomethasone as sealers. Apex of the roots was dipped 1.5 mm intoculture medium for 24 hours in incubator (root dipping technique). The mediumcontact was transformed into confluent chicken fibroblast. Cytotoxicity ofEndomethasone, AH Plus, and Apexit Plus were measured with hemocytometer assay.The results showed that percentage survived cells of Apexit Plus was 68%,Endomethasone 66.42%, and AH Plus 64%. The amount of exist cells were ApexitPlus>Endomethasone>AH Plus. There was no significant difference of cytotoxicityamong the sealers (P>0.05). The conclusion was the amount of exist cells were morethan 50% which means that the three root canal sealers were not toxic.