bluuebunny/crossref_metadata_2025 huggingface dataset:
Veterinary Drug Residues in Animal-Derived Foods: Sample Preparation and Analytical Methods
Authors: Wang Bo , Xie Kaizhou , Lee Kiho
Published: March 2021

Veterinary drugs are used to treat livestock and aquatic diseases and thus are introduced into animal-derived foods, endangering consumer health and safety. Antibiotic resistance is rapidly becoming a major worldwide problem, and there has been a steady increase in the number of pathogens that show multi-drug resistance. Illegal and excessive use of veterinary drugs in animals and aquaculture has serious adverse effects on humans and on all other environmental organisms. It is necessary to develop simple extraction methods and fast analytical methods to effectively detect veterinary drug residues in animal-derived foods. This review summarizes the application of various sample extraction techniques and detection and quantification methods for veterinary drug residues reported in the last decade (2010-2020). This review compares the advantages and disadvantages of various extraction techniques and detection methods and describes advanced methods, such as those that use electrochemical biosensors, piezoelectric biosensors, optical biosensors, and molecularly imprinted polymer biosensors. Finally, the future prospects and trends related to extraction methods, detection methods and advanced methods for the analysis of veterinary drug residues in animal-derived foods are summarized.
Evaluation of Recombinant Kpkt Cytotoxicity on HaCaT Cells: Further Steps towards the Biotechnological Exploitation Yeast Killer Toxins
Authors: Carboni Gavino , Marova Ivana , Zara Giacomo , Zara Severino , Budroni Marilena , Mannazzu Ilaria
Published: March 2021

The soil yeast Tetrapisispora phaffii secretes a killer toxin, named Kpkt, that shows β-glucanase activity and is lethal to wine spoilage yeasts belonging to Kloeckera/Hanseniaspora, Saccharomycodes and Zygosaccharomyces. When expressed in Komagataella phaffii, recombinant Kpkt displays a wider spectrum of action as compared to its native counterpart, being active on a vast array of wine yeasts and food-related bacteria. Here, to gather information on recombinant Kpkt cytotoxicity, lyophilized preparations of this toxin (LrKpkt) were obtained and tested on immortalized human keratinocyte HaCaT cells, a model for the stratified squamous epithelium of the oral cavity and esophagus. LrKpkt proved harmless to HaCaT cells at concentrations up to 36 AU/mL, which are largely above those required to kill food-related yeasts and bacteria in vitro (0.25–2 AU/mL). At higher concentrations, it showed a dose dependent effect that was comparable to that of the negative control and therefore could be ascribed to compounds, other than the toxin, occurring in the lyophilized preparations. Considering the dearth of studies regarding the effects of yeast killer toxins on human cell lines, these results represent a first mandatory step towards the evaluation the possible risks associated to human intake. Moreover, in accordance with that observed on Ceratitis capitata and Musca domestica, they support the lack of toxicity of this toxin on non-target eukaryotic models and corroborate the possible exploitation of killer toxins as natural antimicrobials in the food and beverages industries.
Testing Noncommutativity-Like Model as a Galactic Density Profile
Authors: Ancona-Flores Juan , Hernández-Almada Alberto , García-Aspeitia Miguel
Published: March 2021

Noncommutative-like model (NC-like) is an interesting alternative inspired by string theory to understand and describe the velocity rotation curves of galaxies without the inclusion of dark matter particles. In a natural way, a Gaussian density profile emerges and is characterized by a parameter θ, called the NC-like parameter. Hence we aim to confront the NC-like model with a galaxy sample of the Spitzer Photometry and Accurate Rotation Curves (SPARC) catalog to constrain the model parameters and compare statistically with the Einasto density profile using the Akaike and Bayesian information criteria. According to our results, some galaxies prefer the NC-like over the Einasto model while others do not support NC-like.
Studying the Depth Structure of the Kyrgyz Tien Shan by Using the Seismic Tomography and Magnetotelluric Sounding Methods
Authors: Medved Irina , Bataleva Elena , Buslov Michael
Published: March 2021

This paper presents new results of detailed seismic tomography (ST) on the deep structure beneath the Middle Tien Shan to a depth of 60 km. For a better understanding of the detected heterogeneities, the obtained velocity models were compared with the results of magnetotelluric sounding (MTS) along the Kekemeren and Naryn profiles, running parallel to the 74 and 76 meridians, respectively. We found that in the study region the velocity characteristics and geoelectric properties correlate with each other. The high-velocity high-resistivity anomalies correspond to the parts of the Tarim and Kazakhstan-Junggar plates submerged under the Tien Shan. We revealed that the structure of the Middle Tien Shan crust is conditioned by the presence of the Central Tien Shan microcontinent. It manifests itself as two anomalies lying one below the other: the lower low-velocity low-resistivity anomaly, and the upper high-velocity high-resistivity anomaly. The fault zones, limiting the Central Tien Shan microcontinent, appear as low-velocity low-resistivity anomalies. The obtained features indicate the fluid saturation of the fault zones. According to the revealed features of the Central Tien Shan geological structure, it is assumed that the lower-crustal low-velocity layer can play a significant role in the delamination of the mantle part of the submerged plates.
Bullying Victimization among In-School Adolescents in Ghana: Analysis of Prevalence and Correlates from the Global School-Based Health Survey
Authors: Aboagye Richard Gyan , Seidu Abdul-Aziz , Hagan John Elvis , Frimpong James Boadu , Okyere Joshua , Cadri Abdul , Ahinkorah Bright Opoku
Published: March 2021

(1) Background: Although bullying victimization is a phenomenon that is increasingly being recognized as a public health and mental health concern in many countries, research attention on this aspect of youth violence in low- and middle-income countries, especially sub-Saharan Africa, is minimal. The current study examined the national prevalence of bullying victimization and its correlates among in-school adolescents in Ghana. (2) Methods: A sample of 1342 in-school adolescents in Ghana (55.2% males; 44.8% females) aged 12–18 was drawn from the 2012 Global School-based Health Survey (GSHS) for the analysis. Self-reported bullying victimization “during the last 30 days, on how many days were you bullied?” was used as the central criterion variable. Three-level analyses using descriptive, Pearson chi-square, and binary logistic regression were performed. Results of the regression analysis were presented as adjusted odds ratios (aOR) at 95% confidence intervals (CIs), with a statistical significance pegged at p < 0.05. (3) Results: Bullying victimization was prevalent among 41.3% of the in-school adolescents. Pattern of results indicates that adolescents in SHS 3 [aOR = 0.34, 95% CI = 0.25, 0.47] and SHS 4 [aOR = 0.30, 95% CI = 0.21, 0.44] were less likely to be victims of bullying. Adolescents who had sustained injury [aOR = 2.11, 95% CI = 1.63, 2.73] were more likely to be bullied compared to those who had not sustained any injury. The odds of bullying victimization were higher among adolescents who had engaged in physical fight [aOR = 1.90, 95% CI = 1.42, 2.25] and those who had been physically attacked [aOR = 1.73, 95% CI = 1.32, 2.27]. Similarly, adolescents who felt lonely were more likely to report being bullied [aOR = 1.50, 95% CI = 1.08, 2.08] as against those who did not feel lonely. Additionally, adolescents with a history of suicide attempts were more likely to be bullied [aOR = 1.63, 95% CI = 1.11, 2.38] and those who used marijuana had higher odds of bullying victimization [aOR = 3.36, 95% CI = 1.10, 10.24]. (4) Conclusions: Current findings require the need for policy makers and school authorities in Ghana to design and implement policies and anti-bullying interventions (e.g., Social Emotional Learning (SEL), Emotive Behavioral Education (REBE), Marijuana Cessation Therapy (MCT)) focused on addressing behavioral issues, mental health and substance abuse among in-school adolescents.
Changes of Spatiotemporal Pattern and Network Characteristic in Population Flow under COVID-19 Epidemic
Authors: Li Chengming , Wu Zheng , Zhu Lining , Liu Li , Zhang Chengcheng
Published: March 2021

The COVID-19 pandemic is a major problem facing humanity throughout the world. The rapid and accurate tracking of population flows may therefore be epidemiologically informative. This paper adopts a massive amount of daily population flow data (from January 10 to March 15, 2020) for China obtained from the Baidu Migration platform to analyze the changes of the spatiotemporal patterns and network characteristics in population flow during the pre-outbreak period, outbreak period, and post-peak period. The results show that (1) for temporal characteristics of population flow, the total population flow varies greatly between the three periods, with an overall trend of the pre-outbreak period flow > the post-peak period flow > the outbreak period flow. Impacted by the lockdown measures, the population flow in various provinces plunged drastically and remained low until the post-peak period, at which time it gradually increased. (2) For the spatial pattern, the pattern of population flow is divided by the geographic demarcation line known as the Hu (Heihe-Tengchong) Line, with a high-density interconnected network in the southeast half and a low-density serial-connection network in the northwest half. During the outbreak period, Wuhan city appeared as a hollow region in the population flow network; during the post-peak period, the population flow increased gradually, but it was mainly focused on intra-provincial flow. (3) For the network characteristic changes, during the outbreak period, the gap in the network status between cities at different administrative levels narrowed significantly. Thus, the feasibility of Baidu migration data, comparison with non-epidemic periods, and optimal implications are discussed. This paper mainly described the difference and specific information under non-normal situation compared with existing results under a normal situation, and analyzed the impact mechanism, which can provide a reference for local governments to make policy recommendations for economic recovery in the future under the epidemic period.
Automatic Construction of Indoor 3D Navigation Graph from Crowdsourcing Trajectories
Authors: Fu Xin , Zhang Hengcai , Wang Peixiao
Published: March 2021

Lacking indoor navigation graph has become a bottleneck in indoor applications and services. This paper presents a novel automated indoor navigation graph reconstruction approach from large-scale low-frequency indoor trajectories without any other data sources. The proposed approach includes three steps: trajectory simplification, 2D floor plan extraction and 3D navigation graph construction. First, we propose a ST-Join-Clustering algorithm to identify and simplify redundant stay points embedded in the indoor trajectories. Second, an indoor trajectory bitmap construction based on a self-adaptive Gaussian filter is developed, and we then propose a new improved thinning algorithm to extract 2D indoor floor plans. Finally, we present an improved CFSFDP algorithm with time constraints to identify the 3D topological connection points between two different floors. To illustrate the applicability of the proposed approach, we conducted a real-world case study using an indoor trajectory dataset of over 4000 indoor trajectories and 5 million location points. The case study results showed that the proposed approach improves the navigation network accuracy by 1.83% and the topological accuracy by 13.7% compared to the classical kernel density estimation approach.
Predicting the Global Distribution of Solenopsis geminata (Hymenoptera: Formicidae) under Climate Change Using the MaxEnt Model
Authors: Lee Cheol Min , Lee Dae-Seong , Kwon Tae-Sung , Athar Mohammad , Park Young-Seuk
Published: March 2021

The tropical fire ant Solenopsis geminata (Hymenoptera: Formicidae) is a serious invasive species that causes a decline in agricultural production, damages infrastructure, and harms human health. This study was aimed to develop a model using the maximum entropy (MaxEnt) algorithm to predict the current and future distribution of S. geminata on a global scale for effective monitoring and management. In total, 669 occurrence sites of S. geminata and six bioclimatic variables of current and future climate change scenarios for 2050 and 2100 were used for the modeling. The annual mean temperature, annual precipitation, and precipitation in the driest quarter were the key influential factors for determining the distribution of S. geminata. Although the potential global distribution area of S. geminata is predicted to decrease slightly under global warming, the distribution of favorable habitats is predicted to expand to high latitudes under climate scenarios. In addition, some countries in America and East Asia, such as Brazil, China, South Korea, the USA, and Uruguay, are predicted to be threatened by S. geminata invasion under future climate change. These findings can facilitate the proactive management of S. geminata through monitoring, surveillance, and quarantine measures.
Forensic Entomology in China and Its Challenges
Authors: Wang Yu , Wang Yinghui , Wang Man , Xu Wang , Zhang Yanan , Wang Jiangfeng
Published: March 2021

While the earliest record of forensic entomology originated in China, related research did not start in China until the 1990s. In this paper, we review the recent research progress on the species identification, temperature-dependent development, faunal succession, and entomological toxicology of sarcosaprophagous insects as well as common applications of forensic entomology in China. Furthermore, the difficulties and challenges forensic entomologists face in China are analyzed and possible countermeasures are presented.
Factors Affecting Technical Difficulty in Balloon Enteroscopy-Assisted Endoscopic Retrograde Cholangiopancreatography in Patients with Surgically Altered Anatomy
Authors: Izawa Naoya , Tsuchida Kohei , Tominaga Keiichi , Fukushi Koh , Sakuma Fumi , Kashima Ken , Kunogi Yasuhito , Kanazawa Mimari , Tanaka Takanao , Nagashima Kazunori , Minaguchi Takahito , Iwasaki Mari , Yamamiya Akira , Jinnai Hidehito , Yamabe Akane , Hoshi Koki , Sugaya Takeshi , Iijima Makoto , Goda Kenichi , Irisawa Atsushi
Published: March 2021

Success rates of balloon enteroscopy-assisted endoscopic retrograde cholangiopancreatography (BE-ERCP) for patients with a reconstructed intestinal tract after surgical procedures are unsatisfactory. We retrospectively investigated the factors associated with unsuccessful BE-ERCP. Ninety-one patients who had a reconstructed intestinal tract after gastrectomy or choledochojejunostomy were enrolled. Age, sex, operative method, malignancy, endoscope type, endoscopist’s skill, emergency procedure, and time required to reach the papilla/anastomosis were examined. The primary endpoints were the factors associated with unsuccessful BE-ERCP selective cannulation, while the secondary endpoints were the rate of reaching the papilla/anastomosis, causes of failure to reach the papilla/anastomosis, cannulation success rate, procedure success rate, and rate of adverse events. Younger age (odds ratio, 0.832; 95% CI, 0.706–0.982; p = 0.001) and Roux-en-Y partial gastrectomy (odds ratio, 54.9; 95% CI, 1.09–2763; p = 0.045) were associated with unsuccessful BE- ERCP. The rate of reaching the papilla/anastomosis was 92.3%, the success rate of biliary duct cannulation was 90.5%, procedure success rate was 78.0%, and the rate of adverse events was 5.6%. In conclusion, Roux-en-Y partial gastrectomy and younger age were associated with unsuccessful BE-ERCP. If BE-ERCP is extremely difficult to perform in such patients after Roux-en-Y partial gastrectomy, alternative procedures should be considered early.
Impact of Medication Regimen Simplification on Medication Incidents in Residential Aged Care: SIMPLER Randomized Controlled Trial
Authors: Dugré Nicolas , Bell J. Simon , Hopkins Ria E. , Ilomäki Jenni , Chen Esa Y. H. , Corlis Megan , Van Emden Jan , Hogan Michelle , Sluggett Janet K.
Published: March 2021

In the SImplification of Medications Prescribed to Long-tErm care Residents (SIMPLER) cluster-randomized controlled trial, we investigated the impact of a structured medication regimen simplification intervention on medication incidents in residential aged care facilities (RACFs) over a 12-month follow-up. A clinical pharmacist applied the validated 5-step Medication Regimen Simplification Guide for Residential Aged CarE (MRS GRACE) for 96 of the 99 participating residents in the four intervention RACFs. The 143 participating residents in the comparison RACFs received usual care. Over 12 months, medication incident rates were 95 and 66 per 100 resident-years in the intervention and comparison groups, respectively (adjusted incident rate ratio (IRR) 1.13; 95% confidence interval (CI) 0.53–2.38). The 12-month pre/post incident rate almost halved among participants in the intervention group (adjusted IRR 0.56; 95%CI 0.38–0.80). A significant reduction in 12-month pre/post incident rate was also observed in the comparison group (adjusted IRR 0.67, 95%CI 0.50–0.90). Medication incidents over 12 months were often minor in severity. Declines in 12-month pre/post incident rates were observed in both study arms; however, rates were not significantly different among residents who received and did not receive a one-off structured medication regimen simplification intervention.
Role of Preoperative Assessment in Predicting Tumor-Induced Plasticity in Patients with Diffuse Gliomas
Authors: Latini Francesco , Axelson Hans , Fahlström Markus , Jemstedt Malin , Alberius Munkhammar Åsa , Zetterling Maria , Ryttlefors Mats
Published: March 2021

When diffuse gliomas (DG) affect the brain’s potential to reorganize functional networks, patients can exhibit seizures and/or language/cognitive impairment. The tumor–brain interaction and the individual connectomic organization cannot be predicted preoperatively. We aimed to, first, investigate the relationship between preoperative assessment and intraoperative findings of eloquent tumors in 36 DG operated with awake surgery. Second, we also studied possible mechanisms of tumor-induced brain reorganization in these patients. FLAIR-MRI sequences were used for tumor volume segmentation and the Brain-Grid system (BG) was used as an overlay for infiltration analysis. Neuropsychological (NPS) and/or language assessments were performed in all patients. The distance between eloquent spots and tumor margins was measured. All variables were used for correlation and logistic regression analyses. Eloquent tumors were detected in 75% of the patients with no single variable able to predict this finding. Impaired NPS functions correlated with invasive tumors, crucial location (A4C2S2/A3C2S2-voxels, left opercular-insular/sub-insular region) and higher risk of eloquent tumors. Epilepsy was correlated with larger tumor volumes and infiltrated A4C2S2/A3C2S2 voxels. Language impairment was correlated with infiltrated A3C2S2 voxel. Peritumoral cortical eloquent spots reflected an early compensative mechanism with age as possible influencing factor. Preoperative NPS impairment is linked with high risk of eloquent tumors. A systematic integration of extensive cognitive assessment and advanced neuroimaging can improve our comprehension of the connectomic brain organization at the individual scale and lead to a better oncological/functional balance.
Menstrual Pain and Elasticity of Uterine Cervix
Authors: Xholli Anjeza , Simoncini Gianluca , Vujosevic Sonja , Trombetta Giulia , Chiodini Alessandra , Ferraro Mattia Francesco , Cagnacci Angelo
Published: March 2021

Menstrual pain is consequent to intense uterine contraction aimed to expel menstrual flow through downstream uterine cervix. Herein it was evaluated whether characteristics of uterine cervix are associated with intensity of menstrual pain. Ultrasound elastography was used to analyze cervix elasticity of 75 consecutive outpatient women. Elasticity was related to intensity of menstrual pain defined by a Visual Analogue Scale (VAS). Four regions of interest (ROI) were considered: internal uterine orifice (IUO), anterior (ACC) and posterior cervical (PCC) compartment and middle cervical canal (MCC). Tissue elasticity, evaluated by color score (from 0.5 = blue/violet (low elasticity) to 3.0 = red (high elasticity), and percent tissue deformation was analyzed. Elasticity of IUO was lower (p = 0.0001) than that of MCC or ACC, and it was negatively related (R2 = 0.428; p = 0.0001) to menstrual VAS (CR −2.17; 95%CI −3.80, −0.54; p = 0.01). Presence of adenomyosis (CR 3.24; 95% CI 1.94, 4.54; p = 0.0001) and cervix tenderness at clinical examination (CR 2.74; 95% CI 1.29, 4.20; p = 0.0004), were also independently related to menstrual VAS. At post hoc analysis, women with vs. without menstrual pain had lower IUO elasticity, expressed as color score (0.72 ± 0.40 vs. 0.92 ± 0.42; p = 0.059), lower percent tissue deformation at IUO (0.09 ± 0.05 vs. 0.13 ± 0.08; p = 0.025), a higher prevalence of cervical tenderness at bimanual examination (36.2% vs. 9.5%; p = 0.022) and a higher prevalence of adenomyosis (46.5% vs. 19.9%; p = 0.04). These preliminary data indicate that IUO elasticity is associated with the presence and the intensity of menstrual pain. Mechanisms determining IUO elasticity are useful to be explored.
Polymorphisms in the Gene Encoding Caspase 8 May Predict the Response to First-Line Platinum-Based Chemotherapy in Locally Advanced or Advanced Non-Small-Cell Lung Cancer
Authors: Szczyrek Michał , Mlak Radosław , Szudy-Szczyrek Aneta , Kędziora Karolina , Małecka-Massalska Teresa , Krawczyk Paweł , Milanowski Janusz
Published: March 2021

Caspase 8 is a protein involved in the process of cell apoptosis, which may affect the efficacy of anti-cancer treatment. The aim of our study was to determine the impact of polymorphisms in the CASP-8 gene encoding caspase 8 on the prognosis in non-small-cell lung cancer (NSCLC). The study involved 99 patients with newly diagnosed locally advanced or metastatic NSCLC treated with platinum-based chemotherapy. The presence of the GG genotype was associated with distant metastases, smoking, and a family history of cancer. The higher risk of early progression was associated with weight loss and the CASP-8 genotype (GG vs. AG or AA: 20.51% vs. 2.86%). The higher risk of progression-free survival (PFS) shortening was associated with a higher stage of disease (hazard ratio (HR) = 2.50, 95% CI: 1.61–3.89, p < 0.0001), distant metastases (HR = 2.30, 95% CI: 1.42–3.72, p = 0.0016), and the GG genotype (HR = 1.68, 95% CI: 1.10–2.57, p = 0.0152). The influence of the GG genotype on the PFS was confirmed in a multivariate analysis (HR = 1.80, 95% CI: 1.06–3.05, p = 0.0317). We did not confirm the influence of CASP-8 genotypes on the overall survival (OS).
Prospective Study on Incidence, Risk Factors and Outcome of Recurrent Clostridioides difficile Infections
Authors: Granata Guido , Petrosillo Nicola , Adamoli Lucia , Bartoletti Michele , Bartoloni Alessandro , Basile Gregorio , Bassetti Matteo , Bonfanti Paolo , Borromeo Raffaella , Ceccarelli Giancarlo , De Luca Anna , Di Bella Stefano , Fossati Sara , Franceschini Erica , Gentile Ivan , Giacobbe Daniele , Giacometti Enrica , Ingrassia Fabrizio , Lagi Filippo , Lobreglio Giambattista , Lombardi Andrea , Lupo Laura , Luzzati Roberto , Maraolo Alberto , Mikulska Malgorzata , Mondelli Mario , Mularoni Alessandra , Mussini Cristina , Oliva Alessandra , Pandolfo Alessandro , Rogati Carlotta , Trapani Filippo , Venditti Mario , Viale Pierluigi , Caraffa Emanuela , Cataldo Maria ,   on behalf of the ReCloDi (Recurrence of Clostridioides difficile Infection) Study Group
Published: March 2021

Background: Limited and wide-ranging data are available on the recurrent Clostridioides difficile infection (rCDI) incidence rate. Methods: We performed a cohort study with the aim to assess the incidence of and risk factors for rCDI. Adult patients with a first CDI, hospitalized in 15 Italian hospitals, were prospectively included and followed-up for 30 d after the end of antimicrobial treatment for their first CDI. A case–control study was performed to identify risk factors associated with 30-day onset rCDI. Results: Three hundred nine patients with a first CDI were included in the study; 32% of the CDI episodes (99/309) were severe/complicated; complete follow-up was available for 288 patients (19 died during the first CDI episode, and 2 were lost during follow-up). At the end of the study, the crude all-cause mortality rate was 10.7% (33 deaths/309 patients). Two hundred seventy-one patients completed the follow-up; rCDI occurred in 21% of patients (56/271) with an incidence rate of 72/10,000 patient-days. Logistic regression analysis identified exposure to cephalosporin as an independent risk factor associated with rCDI (RR: 1.7; 95% CI: 1.1–2.7, p = 0.03). Conclusion: Our study confirms the relevance of rCDI in terms of morbidity and mortality and provides a reliable estimation of its incidence.
An Update on the Role of Ubiquitination in Melanoma Development and Therapies
Authors: Soysouvanh Frédéric , Giuliano Serena , Habel Nadia , El-Hachem Najla , Pisibon Céline , Bertolotto Corine , Ballotti Robert
Published: March 2021

The ubiquitination system plays a critical role in regulation of large array of biological processes and its alteration has been involved in the pathogenesis of cancers, among them cutaneous melanoma, which is responsible for the most deaths from skin cancers. Over the last decades, targeted therapies and immunotherapies became the standard therapeutic strategies for advanced melanomas. However, despite these breakthroughs, the prognosis of metastatic melanoma patients remains unoptimistic, mainly due to intrinsic or acquired resistances. Many avenues of research have been investigated to find new therapeutic targets for improving patient outcomes. Because of the pleiotropic functions of ubiquitination, and because each step of ubiquitination is amenable to pharmacological targeting, much attention has been paid to the role of this process in melanoma development and resistance to therapies. In this review, we summarize the latest data on ubiquitination and discuss the possible impacts on melanoma treatments.
Threat Posed by Future Sea-Level Rise to Freshwater Resources in the Upper Pearl River Estuary
Authors: Wang Jiaxi , Hong Bo
Published: March 2021

The degradation of densely populated river delta environments due to the accelerating rise in sea level can affect the availability of freshwater for municipal supplies, irrigation, and industrial use. A fully calibrated three-dimensional numerical model is used in this study to evaluate the threat posed by the sea-level rise, which predicted to occur by 2100, to freshwater resources in the upper tributaries of Pearl River Estuary. The results indicate that both the intensity and duration of dry-season saltwater intrusion greatly increase as the sea level rises, making the water at drinking-water intake stations for the four waterworks no longer suitable for municipal supply. Flow modulation is performed to identify the threshold at which saltwater intrusion could be effectively suppressed in response to both sea-level rise and dry season hydrodynamics. The number of days for which water meets the drinking-water standard decreases as the sea level rises, but increases with increased river flow. The combined effect of future drought and sea-level rise would further limit the availability of freshwater in the upper tributaries. Stronger upstream salinity transport during flood tide are found in the sea-level rise case. The increased flood tidal salinity transport would have great impact on the tidal freshwater wetlands.
Design, Performance Evaluation and Field Test of a Water Jet Tool for ROV Trencher
Authors: Li Ji-Hong , Lee Mun-Jik , Kang Hyungjoo , Kim Min-Gyu , Cho Gun
Published: March 2021

ROV trencher is a kind of ROV which trenches the sea floor using a specifically designed tool and buries the subsea cables and pipelines. According to the soil conditions, this trenching method can have two different types, one is mechanical cutting and the other one is water jetting. In this paper, we present a water jet tool design method for a 2500 m depth-rated ROV trencher. A series of CFD simulations and laboratory tests with one nozzle, and a ground test using 1:6 scale jetting arm model were carried out to derive and demonstrate the jetting tool design parameters. In October 2018, the constructed ROV trencher was put into the sea trial in the East Sea of Korea to evaluate its final performances. In addition, in December 2019, the trencher was applied in a construction site to bury subsea water pipelines near the Yogji Island in the Korea. Through these two field tests and operation, the trencher was demonstrated for both its operational capability and trenching performance. The main contribution of this paper is that it presents the entire design procedures of water jet tools, including CFD simulations, laboratory tests, field test with 1:6 scaled jetting tool, and the final prototype tool design. These consecutive procedures are carried out in order for us to set up sort of relationship between jetting angle, trench depth, trench speed, and jetting power, from which we can predict and evaluate the trenching performance of the prototype jetting tool.
Innovations in Multi-Physics Methods Development, Validation, and Uncertainty Quantification
Authors: Avramova Maria , Abarca Agustin , Hou Jason , Ivanov Kostadin
Published: March 2021

This paper provides a review of current and upcoming innovations in development, validation, and uncertainty quantification of nuclear reactor multi-physics simulation methods. Multi-physics modelling and simulations (M&S) provide more accurate and realistic predictions of the nuclear reactors behavior including local safety parameters. Multi-physics M&S tools can be subdivided in two groups: traditional multi-physics M&S on assembly/channel spatial scale (currently used in industry and regulation), and novel high-fidelity multi-physics M&S on pin (sub-pin)/sub-channel spatial scale. The current trends in reactor design and safety analysis are towards further development, verification, and validation of multi-physics multi-scale M&S combined with uncertainty quantification and propagation. Approaches currently applied for validation of the traditional multi-physics M&S are summarized and illustrated using established Nuclear Energy Agency/Organization for Economic Cooperation and Development (NEA/OECD) multi-physics benchmarks. Novel high-fidelity multi-physics M&S allow for insights crucial to resolve industry challenge and high impact problems previously impossible with the traditional tools. Challenges in validation of novel multi-physics M&S are discussed along with the needs for developing validation benchmarks based on experimental data. Due to their complexity, the novel multi-physics codes are still computationally expensive for routine applications. This fact motivates the use of high-fidelity novel models and codes to inform the low-fidelity traditional models and codes, leading to improved traditional multi-physics M&S. The uncertainty quantification and propagation across different scales (multi-scale) and multi-physics phenomena are demonstrated using the OECD/NEA Light Water Reactor Uncertainty Analysis in Modelling benchmark framework. Finally, the increasing role of data science and analytics techniques in development and validation of multi-physics M&S is summarized.
Telomere Length Dynamics and Chromosomal Instability for Predicting Individual Radiosensitivity and Risk via Machine Learning
Authors: Luxton Jared J. , McKenna Miles J. , Lewis Aidan M. , Taylor Lynn E. , Jhavar Sameer G. , Swanson Gregory P. , Bailey Susan M.
Published: March 2021

The ability to predict a cancer patient’s response to radiotherapy and risk of developing adverse late health effects would greatly improve personalized treatment regimens and individual outcomes. Telomeres represent a compelling biomarker of individual radiosensitivity and risk, as exposure can result in dysfunctional telomere pathologies that coincidentally overlap with many radiation-induced late effects, ranging from degenerative conditions like fibrosis and cardiovascular disease to proliferative pathologies like cancer. Here, telomere length was longitudinally assessed in a cohort of fifteen prostate cancer patients undergoing Intensity Modulated Radiation Therapy (IMRT) utilizing Telomere Fluorescence in situ Hybridization (Telo-FISH). To evaluate genome instability and enhance predictions for individual patient risk of secondary malignancy, chromosome aberrations were assessed utilizing directional Genomic Hybridization (dGH) for high-resolution inversion detection. We present the first implementation of individual telomere length data in a machine learning model, XGBoost, trained on pre-radiotherapy (baseline) and in vitro exposed (4 Gy γ-rays) telomere length measurements, to predict post radiotherapy telomeric outcomes, which together with chromosomal instability provide insight into individual radiosensitivity and risk for radiation-induced late effects.
Achieving Win–Win Solutions in Telecoupled Human–Land Systems
Authors: Guo Xiaona , Chen Ruishan , Li Qiang , Meadows Michael E.
Published: March 2021

Telecoupling refers to socioeconomic and environmental interactions between distant places. Telecoupling is becoming even more significant in the increasingly globalized world and it plays a key role in the emergence of major global environmental problems. In particular, it contributes to land degradation and the achievement of the United Nations’ Sustainable Development Goals (SDGs). However, there is a lack of systematic examination of the impacts of telecoupling on land system change, and how to respond to the undesirable impacts. Based on CiteSpace Software, here we analyze the current research status of telecoupled human–land systems, including publications, major scientific research institutions, and research processes. We explore the impacts of telecoupling on land and how to respond to these impacts. Finally, we propose a framework that is composed of impact identification, system integration, and responses to achieve a win-win situation in telecoupled human–land systems. The framework can help to create a sustainable future for telecoupled human–land systems.
Quantification of Tackiness of a Grease: The Road to a Method
Authors: Georgiou Emmanuel P. , Drees Dirk , De Bilde Michel , Anderson Michael , Carlstedt Matthias , Mollenhauer Olaf
Published: March 2021

In this work, we report on the most recent progress in studying temperature influence on tackiness of greases, as well as the reproducibility of the method. Tackiness and adhesion of greases have been identified as key intrinsic properties that can influence their functionality and performance. During the last eight years, a reliable method to quantify the tackiness and adhesion of greases has evolved from an experimental lab-scale set-up towards a standardised approach, including an ASTM method and a dedicated test tool. The performance of lubricating greases—extensively used in diverse industrial applications—is strongly dependent on their adherence to the substrate, cohesion and thread formation or tackiness of the greases. This issue attracts more and more industrial interest as the complexity in grease formulation evolves and it is harder to differentiate between available greases. With this method, grease formulators will have an efficient measurement tool to support their work.
Global Dynamics of a Discrete-Time MERS-Cov Model
Authors: DarAssi Mahmoud H. , Safi Mohammad A. , Ahmad Morad
Published: March 2021

In this paper, we have investigated the global dynamics of a discrete-time middle east respiratory syndrome (MERS-Cov) model. The proposed discrete model was analyzed and the threshold conditions for the global attractivity of the disease-free equilibrium (DFE) and the endemic equilibrium are established. We proved that the DFE is globally asymptotically stable when R0≤1. Whenever R˜0>1, the proposed model has a unique endemic equilibrium that is globally asymptotically stable. The theoretical results are illustrated by a numerical simulation.
Optimization and Simulation of Dynamic Performance of Production–Inventory Systems with Multivariable Controls
Authors: AL-Khazraji Huthaifa , Cole Colin , Guo William
Published: March 2021

The production–inventory system is a problem of multivariable input and multivariant output in mathematics. Selecting the best system control parameters is a crucial managerial decision to achieve and dynamically maintain an optimal performance in terms of balancing the order rate and stock level under dynamic influence of many factors affecting the system operations. The dynamic performance of the popular APIOBPCS model and the newly modified 2APIOBPCS model for optimal control of production–inventory systems is examined in the study. This examination is based on the leveled ground with a new simulation scheme that incorporates a designated multi-objective particle swarm optimization (MOPSO) algorithm into the simulation, which enables the optimal set of system control parameters to be selected for achieving the situational best possible performance of the production–inventory system under study. The dynamic performance is measured by the variance ratio between the order rate and the sales rate related to the bullwhip effect, and the integral of absolute error related to the inventory responsiveness in response to a random customer demand. Our simulation indicates that the 2APIOBPCS model performed better than or at least no worse than, and more robust than the APIOBPCS model under different conditions.
Designing Tasks for Introducing Functions and Graphs within Dynamic Interactive Environments
Authors: Antonini Samuele , Lisarelli Giulia
Published: March 2021

In this paper, we elaborate on theoretical and methodological considerations for designing a sequence of tasks for introducing middle and high school students to functions and their graphs. In particular, we present didactical activities with an artifact realized within a dynamic interactive environment and having the semiotic potential for embedding mathematical meanings of covariation of independent and dependent variables. After laying down the theoretical grounds, we formulate the design principles that emerged as the result of bringing the theory into a dialogue with the didactical aims. Finally, we present a teaching sequence, designed and implemented on the basis of the design principles and we show how students’ efforts in describing and manipulating the different graphs of functions can promote their production of specific signs that can progressively evolve towards mathematical meanings.
Numerical Modeling of the Spread of Cough Saliva Droplets in a Calm Confined Space
Authors: Chillón Sergio A. , Ugarte-Anero Ainara , Aramendia Iñigo , Fernandez-Gamiz Unai , Zulueta Ekaitz
Published: March 2021

The coronavirus disease 2019 (COVID-19) outbreak has altered the lives of everyone on a global scale due to its high transmission rate. In the current work, the droplet dispersion and evaporation originated by a cough at different velocities is studied. A multiphase computational fluid dynamic model based on fully coupled Eulerian–Lagrangian techniques was used. The evaporation, breakup, mass transfer, phase change, and turbulent dispersion forces of droplets were taken into account. A computational domain imitating an elevator that with two individuals inside was modeled. The results showed that all droplets smaller than 150 μm evaporate before 10 s at different heights. Smaller droplets of <30 µm evaporate quickly, and their trajectories are governed by Brownian movements. Instead, the trajectories of medium-sized droplets (30–80 µm) are under the influence of inertial forces, while bigger droplets move according to inertial and gravitational forces. Smaller droplets are located in the top positions, while larger (i.e., heaviest) droplets are located at the bottom.
Objectivation of an Educational Model in Cranial Osteopathy Based on Experience
Authors: Requena-García Jesús , García-Nieto Evelyn , Varillas-Delgado David
Published: March 2021

Background and Objectives: The techniques directed to the cranial field in osteopathy are the most questioned due to the lack of scientific evidence. In osteopathic practice, manual palpation is essential and, therefore, measuring reliability is fundamental. The objective of this study is to assess the precision and objectification of an educational model in cranial osteopathy based on experience. Materials and Methods: A reliability study was conducted in a cadaver skull where a strain gauge was placed on the sphenobasilar synchondrosis (SBS) of the base of the skull. Three cranial osteopathic techniques (lateral compression, anteroposterior compression, and compression maneuver of the mastoids) were performed 25 times by osteopaths with different degrees of experience (5–10 years, 1–5 years, <1 year). Measurements were computed for each of the three techniques of each group in comparison with the osteopath with >15 years of experience. Data were analyzed to check for inter- and intra-observer reliability using intra-class correlation coefficients (ICC). Results: Reliability in osteopaths with 5–10 years’ experience (observer 1 and observer 2) performing all three techniques was higher (p < 0.001) than the osteopath with >15 years’ experience. Little or no reliability were observed in osteopaths with less experience. Conclusions: The experience of the osteopaths determines the reliability and effectiveness of the cranial techniques, a fundamental part in objectifying these techniques. This model can help implement objective training in cranial osteopathy formation.
Strong Genetic Effects on Bone Mineral Density in Multiple Locations with Two Different Techniques: Results from a Cross-Sectional Twin Study
Authors: Piroska Marton , Tarnoki David Laszlo , Szabo Helga , Jokkel Zsofia , Meszaros Szilvia , Horvath Csaba , Tarnoki Adam Domonkos
Published: March 2021

Background and Objectives: Previous studies have demonstrated that risk of hip fracture is at least partly heritable. The aim of this study was to determine the magnitude of the genetic component of bone mineral density (BMD), using both X-ray and ultrasound assessment at multiple sites. Materials and Methods: 216 adult, healthy Hungarian twins (124 monozygotic, MZ, 92 dizygotic, DZ; mean age 54.2 ± 14.3 years), recruited from the Hungarian Twin Registry with no history of oncologic disease underwent cross-sectional BMD studies. We measured BMD, T- and Z-scores with dual energy X-ray absorptiometry (DEXA) at multiple sites (lumbar spine, femoral neck, total hip and radius). Quantitative bone ultrasound (QUS) was also performed, resulting in a calculated value of estimated bone mineral density (eBMD) in the heel bone. Heritability was calculated using the univariate ACE model. Results: Bone density had a strong genetic component at all sites with estimates of heritability ranging from 0.613 to 0.838 in the total sample. Lumbar BMD and calcaneus eBMD had major genetic components with estimates of 0.828 and 0.838 respectively, and least heritable (0.653) at the total hip. BMD of the radius had also a strong genetic component with an estimate of 0.806. No common environmental effect was found. The remaining variance was influenced by unique environment (0.162 to 0.387). In females only, slightly higher additive genetic estimates were found, especially in the case of the femoral neck and total hip. Conclusion: Bone mineral density is strongly heritable, especially in females at all locations using both DEXA and QUS, which may explain the importance of family history as a risk factor for bone fractures. Unshared environmental effects account for the rest of the variance with slight differences in magnitude across various bone regions, supporting the role of lifestyle in preventing osteoporotic fractures with various efficacy in different bone regions.
Investigating Different Forms of Hydrogen Sulfide in Cerebrospinal Fluid of Various Neurological Disorders
Authors: Greco Viviana , Neri Cristina , Pieragostino Damiana , Spalloni Alida , Persichilli Silvia , Gastaldi Matteo , Mercuri Nicola B. , Longone Patrizia , Urbani Andrea
Published: March 2021

Over the past 30 years a considerable amount of data has accumulated on the multifaceted role of hydrogen sulfide (H2S) in the central nervous system. Depending on its concentrations, H2S has opposite actions, ranging from neuromodulator to neurotoxic. Nowadays, accurate determination of H2S is still an important challenge to understand its biochemistry and functions. In this perspective, this study aims to explore H2S levels in cerebrospinal fluid (CSF), key biofluid for neurological studies, and to assess alleged correlations with neuroinflammatory and neurodegenerative mechanisms. A validated analytical determination combining selective electrochemical detection with ion chromatography was developed to measure free and bound sulfur forms of H2S. A first cohort of CSF samples (n = 134) was analyzed from patients with inflammatory and demyelinating disorders (acute disseminated encephalomyelitis; multiple sclerosis), chronic neurodegenerative diseases (Alzheimer disease; Parkinson disease), and motor neuron disease (Amyotrophic lateral sclerosis). Given its analytical features, the chromatographic method resulted sensitive, reproducible and robust. We also explored low molecular weight-proteome linked to sulphydration by proteomics analysis on matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). This study is a first clinical report on CSF H2S concentrations from neurological diseases and opens up new perspectives on the potential clinical relevance of H2S and its potential therapeutic application.
YAP and TAZ Mediators at the Crossroad between Metabolic and Cellular Reprogramming
Authors: Di Benedetto Giorgia , Parisi Silvia , Russo Tommaso , Passaro Fabiana
Published: March 2021

Cell reprogramming can either refer to a direct conversion of a specialized cell into another or to a reversal of a somatic cell into an induced pluripotent stem cell (iPSC). It implies a peculiar modification of the epigenetic asset and gene regulatory networks needed for a new cell, to better fit the new phenotype of the incoming cell type. Cellular reprogramming also implies a metabolic rearrangement, similar to that observed upon tumorigenesis, with a transition from oxidative phosphorylation to aerobic glycolysis. The induction of a reprogramming process requires a nexus of signaling pathways, mixing a range of local and systemic information, and accumulating evidence points to the crucial role exerted by the Hippo pathway components Yes-Associated Protein (YAP) and Transcriptional Co-activator with PDZ-binding Motif (TAZ). In this review, we will first provide a synopsis of the Hippo pathway and its function during reprogramming and tissue regeneration, then we introduce the latest knowledge on the interplay between YAP/TAZ and metabolism and, finally, we discuss the possible role of YAP/TAZ in the orchestration of the metabolic switch upon cellular reprogramming.