bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of Determinants Affecting Consumer Purchase Intentions for Mackerel in the Korean Domestic Market
Authors: Kim Jonghwa
Published: August 2024

Mackerel is one of the most popular fish in Korea. However, the determinants of consumer purchase behaviors have not been identified, even though mackerel is an important fish to Korean consumers. This study aims to predict the determinants affecting consumer purchase intentions for mackerel in the Korean domestic market. For this, the author conducted a questionnaire survey, predicted the consumers’ purchase intentions for mackerel using PLS-SEM (partial least squares structural equation modeling), and evaluated the predictions using the TPB (theory of planned behavior) model. Moreover, this study examined whether there is a difference in consumers’ purchase intentions between Korean and Norwegian mackerel. As a result, it is identified that the quality and utility of exogenous constructs indirectly affected the purchase intention (PI) through attitude toward the behavior (AB). In addition, the PI was affected by the subject norm (SN), which was affected considerably by the industrial conditions (IC) of exogenous constructs. Furthermore, the PI was also influenced by perceived behavioral control (PBC), which has the strongest impact on the PI. However, the purchase determinants of consumers were not different between Korean and Norwegian mackerel. In conclusion, Korean consumers consider quality and utility when they purchase mackerel, and their positive attitude can help them make a purchasing decision. Moreover, the more they recognize the conditions of production, business, and culture, the higher their intention is to purchase mackerel because they are forming a positive social atmosphere. Furthermore, whether they have the financial leeway and repulsion also represents factors in their purchase intention for mackerel. Finally, although Korean consumers prefer domestic seafood, they do not have different preferences when purchasing mackerel.
Pueraria lobata–Prunus mume Complex Alleviates Alcoholic Liver Disease by Regulating Lipid Metabolism and Inhibiting Inflammation: A Transcriptome and Gut Microbiota Analysis
Authors: Gao Ruixi , Huang Qi , Zeng Yanfeng , Chen Dandan , Jia Ziming , Han Bingchen , Huang Xianju , Wang Qiang , Hu Xin , Liao Maochuan , Li Jun
Published: August 2024

Background: Lipid metabolism disorder appears to be one of the early features of alcoholic liver disease (ALD), which can be speculated via omics analysis including liver transcriptomics and gut microbiota. A complex consisting of the roots of Pueraria lobata and dried fruits of Prunus mume (PPC), which possesses hepatoprotective effects, could serve as a drug or functional food. The lack of non-polysaccharide compounds in PPC with their moderation effects on gut microbiota suggests the necessity for a relevant study. Methods: Six groups of Kunming mice (control, Baijiu injury, silybin, low, medium, and high) were modelled by gavage with Baijiu (for 14 days) and PPC (equivalent to a maximum dose of 9 g/kg in humans). The liver transcriptome data were analyzed to predict gene annotation, followed by the verification of gut microbiota, serum, tissue staining, immunohistochemistry, and Western blotting. Liquid chromatography-mass spectrometry was used to detect the components. Results: PPC normalized serum ALT (40 U/L), down-regulated TLR4-NF-κB signaling pathway to inhibit the release of TNF-α (90 pg/mL), improved the expression of occludin, claudin-4, and ZO-1, and restored the abundance of Muribaculaceae, Bacteroides and Streptococcus. Conclusion: PPC can alleviate ALD by regulating the gut microbiota with an anti-inflammatory and intestinal barrier, and has an application value in developing functional foods.
Strategies to Improve the Functional Value of Meat and Meat Products
Authors: Jaworska Danuta , Sadowka Anna
Published: August 2024

Meat is a necessary component of the human diet because of its unique chemical composition, nutritional value, and complete protein content with favourable proportions of amino acids [...]
Bioinformatics Analysis of the Genome of Rhodococcus rhodochrous IEGM 1362, an (−)-Isopulegol Biotransformer
Authors: Maltseva Polina Yu. , Plotnitskaya Natalia A. , Krivoruchko Anastasiia V. , Beletskiy Aleksey V. , Rakitin Andrey L. , Mardanov Andrey V. , Ivshina Irina B.
Published: August 2024

A genome of Rhodococcus rhodochrous IEGM 1362 was sequenced and annotated. This strain can transform monoterpene alcohol (–)-isopulegol with the formation of two novel pharmacologically promising metabolites. Nine genes encoding cytochrome P450, presumably involved in (–)-isopulegol transformation, were found in the genome of R. rhodochrous IEGM 1362. Primers and PCR conditions for their detection were selected. The obtained data can be used for the further investigation of genes encoding enzymes involved in monoterpene biotransformation.
The Clinical Heterogeneity of Spinal Muscular Atrophy with Respiratory Distress Type 1 (SMARD1)—A Report of Three Cases, Including Twins
Authors: Leśniak Alicja , Glińska Marta , Patalan Michał , Ostrowska Iwona , Świrska-Sobolewska Monika , Giżewska-Kacprzak Kaja , Kotkowiak Agata , Leśniak Anna , Walczak Mieczysław , Śmigiel Robert , Giżewska Maria
Published: August 2024

Spinal muscular atrophy with respiratory distress type 1 (SMARD1; OMIM #604320, ORPHA:98920) is a rare autosomal recessive congenital motor neuron disease. It is caused by variants in the IGHMBP2 gene. Clinically, it presents with respiratory failure due to diaphragmatic paralysis, progressive muscle weakness starting in the distal parts of the limbs, dysphagia, and damage to sensory and autonomic nerves. Unlike spinal muscular atrophy (SMA), SMARD1 has a distinct genetic etiology and is not detected in the population newborn screening programs. Most children with SMARD1 do not survive beyond the first year of life due to progressive respiratory failure. Artificial ventilation can prolong survival, but no specific treatment is available. Therapy focuses on mechanical ventilation and improving the patient’s quality of life. Research into gene therapy is ongoing. We report three female patients with SMARD1, including twins from a triplet pregnancy. In twin sisters (patient no. 1 and patient no. 2), two heterozygous variants in the IGHMBP2 gene were identified: c.595G>C/p.Ala199Pro and c.1615_1623del/p.Ser539_Tyr541del. In patient no. 3, a variant c.1478C>T/p.Thr493Ile and a variant c.439C>T/p.Arg147* in the IGHMBP2 gene were detected. Our findings underscore the variability of clinical presentations, even among patients sharing the same pathogenic variants in the IGHMBP2 gene, and emphasize the importance of early genetic diagnosis in patients presenting with respiratory failure, with or without associated diaphragmatic muscle paralysis.
RETRACTED: Kosumi et al. Systemic Retinoids for Generalized Verrucosis Due to Congenital Immunodeficiency: Case Reports and Review of the Literature. Genes 2023, 14, 769
Authors: Kosumi Hideyuki , Natsuga Ken , Yanagi Teruki , Ujiie Hideyuki
Published: August 2024

The journal Genes retracts the article, “Systemic Retinoids for Generalized Verrucosis Due to Congenital Immunodeficiency: Case Reports and Review of the Literature” [...]
Role of Selected Genetic Polymorphisms in the Development of Rheumatoid Arthritis in a British White Population
Authors: Mastana Sarabjit , Knight Ella , Hampson Abigail , Akam Liz , Hunter David John , Ghelani Anant , Samanta Ash , Singh Puneetpal
Published: August 2024

Background: Rheumatoid arthritis (RA) is a complex autoimmune disease that negatively affects synovial joints, leading to the deterioration of movement and mobility of patients. This chronic disease is considered to have a strong genetic inheritance, with genome-wide association studies (GWAS) highlighting many genetic loci associated with the disease. Moreover, numerous confounding and non-genetic factors also contribute to the risk of the disease. Aims: This study investigates the association of selected genetic polymorphisms with rheumatoid arthritis risk and develops a polygenic risk score (PRS) based on selected genes. Methods: A case-control study recruited fully consenting participants from the East Midlands region of the UK. DNA samples were genotyped for a range of polymorphisms and genetic associations were calculated under several inheritance models. PRS was calculated at crude (unweighted) and weighted levels, and its associations with clinical parameters were determined. Results: There were significant associations with the risk of RA at six genetic markers and their associated risk alleles (TNRF2*G, TRAF1*A, PTPN22*T, HLA-DRB1*G, TNFα*A, and IL4-590*T). The TTG haplotype at the VDR locus increased the risk of RA with an OR of 3.05 (CI 1.33–6.98, p = 0.009). The GA haplotype of HLADRB1-TNFα-308 was a significant contributor to the risk of RA in this population (OR = 2.77, CI 1.23–6.28, p = 0.01), although linkage disequilibrium was low. The polygenic risk score was significantly higher in cases over controls in both unweighted (mean difference = 1.48, t285 = 5.387, p < 0.001) and weighted (mean difference = 2.75, t285 = 6.437, p < 0.001) results. Conclusion: Several genetic loci contribute to the increased risk of RA in the British White sample. The PRS is significantly higher in those with RA and can be used for clinical applications and personalised prevention of disease.
Changes in the Transcriptome and Long Non-Coding RNAs but Not the Methylome Occur in Human Cells Exposed to Borrelia burgdorferi
Authors: Berthold Anne , Lloyd Vett K.
Published: August 2024

Lyme disease, caused by infection with members of the Lyme borreliosis group of Borrelia spirochete bacteria, is increasing in frequency and distribution worldwide. Epigenetic interactions between the mammalian host, tick, and bacterial pathogen are poorly understood. In this study, high-throughput next-generation sequencing (NGS) allowed for the in vitro study of the transcriptome, non-coding RNAs, and methylome in human host cells in response to Borrelia burgdorferi infection. We tested the effect of the Borrelia burgdorferi strain B31 on a human primary cell line (HUVEC) and an immortalized cell line (HEK-293) for 72 h, a long-duration time that might allow for epigenetic responses in the exposed human host cells. Differential gene expression was detected in both cell models in response to B. burgdorferi. More differentially expressed genes were found in HUVECs compared to HEK-293 cells. Borrelia burgdorferi exposure significantly induced genes in the interferon, in addition to cytokine and other immune response signaling in HUVECs. In HEK-293 cells, pre-NOTCH processing in Golgi was significantly downregulated in Borrelia-exposed cells. Other significantly altered gene expressions were found in genes involved in the extracellular matrix. No significant global methylation changes were detected in HUVECs or HEK-293 cells exposed to B. burgdorferi; however, two long non-coding RNAs and a pseudogene were deregulated in response to B. burgdorferi in HUVECs, suggesting that other epigenetic mechanisms may be initiated by infection.
Whole-Exome Analysis for Polish Caucasian Patients with Retinal Dystrophies and the Creation of a Reference Genomic Database for the Polish Population
Authors: Matczyńska Ewa , Szymańczak Robert , Stradomska Katarzyna , Łyszkiewicz Przemysław , Jędrzejowska Maria , Kamińska Karolina , Beć-Gajowniczek Marta , Suchecka Ewa , Zagulski Marek , Wiącek Marta , Wylęgała Edward , Machalińska Anna , Mossakowska Małgorzata , Puzianowska-Kuźnicka Monika , Teper Sławomir , Boguszewska-Chachulska Anna
Published: August 2024

We present the results of the first study of a large cohort of patients with inherited retinal dystrophies (IRD) performed for the Polish population using whole-exome sequencing (WES) in the years 2016–2019. Moreover, to facilitate such diagnostic analyses and enable future application of gene therapy and genome editing for IRD patients, a Polish genomic reference database (POLGENOM) was created based on whole-genome sequences of healthy Polish Caucasian nonagenarians and centenarians. The newly constructed database served as a control, providing a comparison for variant frequencies in the Polish population. The diagnostic yield for the selected group of IRD patients reached 64.9%. The study uncovered the most common pathogenic variants in ABCA4 and USH2A in the European population, along with several novel causative variants. A significant frequency of the ABCA4 complex haplotype p.(Leu541Pro; Ala1038Val) was observed, as well as that of the p.Gly1961Glu variant. The first VCAN causative variant NM_004385.5:c.4004-2A>G in Poland was found and described. Moreover, one of the first patients with the RPE65 causative variants was identified, and, in consequence, could receive the dedicated gene therapy. The availability of the reference POLGENOM database enabled comprehensive variant characterisation during the NGS data analysis, confirming the utility of a population-specific genomic database for enhancing diagnostic accuracy. Study findings suggest the significance of genetic testing in elder patients with unclear aetiology of eye diseases. The combined approach of NGS and the reference genomic database can improve the diagnosis, management, and future treatment of IRDs.
Interspecific and Intraspecific Transcriptomic Variations Unveil the Potential High-Altitude Adaptation Mechanisms of the Parnassius Butterfly Species
Authors: Ding Chen , Su Chengyong , Li Yali , Zhao Youjie , Wang Yunliang , Wang Ying , Nie Ruie , He Bo , Ma Junye , Hao Jiasheng
Published: August 2024

Parnassius butterflies have significantly advanced our understanding of biogeography, insect–plant interactions, and other fields of ecology and evolutionary biology. However, to date, little is known about the gene expression patterns related to the high-altitude adaptation of Parnassius species. In this study, we obtained high-throughput RNA-seq data of 48 adult Parnassius individuals covering 10 species from 12 localities in China, and deciphered their interspecific and intraspecific expression patterns based on comparative transcriptomic analyses. Though divergent transcriptional patterns among species and populations at different altitudes were found, a series of pathways related to genetic information processing (i.e., recombination, repair, transcription, RNA processing, and ribosome biogenesis), energy metabolism (i.e., oxidative phosphorylation, thermogenesis, and the citrate cycle), and cellular homeostasis were commonly enriched, reflecting similar strategies to cope with the high-altitude environments by activating energy metabolism, enhancing immune defense, and concurrently inhibiting cell growth and development. These findings deepen our understanding about the molecular mechanisms of adaptative evolution to extreme environments, and provide us with some theoretical criteria for the biodiversity conservation of alpine insects.
Genome-Editing Products Line up for the Market: Will Europe Harvest the Benefits from Science and Innovation?
Authors: Polidoros Alexios , Nianiou-Obeidat Irini , Tsakirpaloglou Nikolaos , Petrou Nestor , Deligiannidou Eleftheria , Makri Nefeli-Maria
Published: August 2024

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technologies have revolutionized genome editing, significantly advancing the improvement of cultivated crop species. This review provides an overview of genome-edited crops that have either reached the market or received the necessary approvals but are not yet available to consumers. We analyze various genome-editing studies to understand the distribution of different genome-editing systems, the types of site-directed nucleases employed, and the geographical spread of these studies, with a specific focus on global and European contexts. Additionally, we examine the target crops involved. The review also outlines the multiple steps required for the legal acceptance of genome-edited crops within European jurisdictions. We conclude with suggestions for the future prospects of genome-editing research in Europe, aiming to streamline the approval process and enhance the development and adoption of genome-edited crops.
Analogical Perspective from “Shengsheng” Philosophy on Virginia Hamilton’s Survival Writing in M.C. Higgins, the Great
Authors: Liu Huimin
Published: August 2024

This article aims at examining Virginia Hamilton’s survival writing in the novel M.C. Higgins, the Great through the analogical lens with the traditional Chinese philosophy of “shengsheng (生生)”. Current research on Hamilton’s survival writing has ignored the cosmological aspect. In fact, what the novel reveals is not limited to the aspects of social and emotional survival, but also the ecological or cosmical co-existence. Considering Hamilton’s global awareness and some similarities between African and Chinese traditions, this article resorts to the cross-cultural reference of the Chinese “shengsheng” philosophy. The concept originating from Xici (《系辞》), the commentaries on Zhouyi (《周易》), is well known for its wisdom on how all things in the universe can be born and how they can coexist, and thus it can be drawn upon for exploring Hamilton’s survival writing. Specifically, this article takes a comprehensive analogical examination and discussion of the four aspects, namely, shengsheng virtue (生生之德), shengsheng affect (生生之情), shengsheng disposition (生生之性), and shengsheng fate (生生之命). This is to supplement the covering of Hamilton’s survival writing and to enlarge the interpretation of Hamilton’s works with philosophical and cosmopolitan visions.
A Focus on Heart Failure Management through Diet and Nutrition: A Comprehensive Review
Authors: Liao Lee P. , Pant Anushriya , Marschner Simone , Talbot Peter , Zaman Sarah
Published: August 2024

There is emerging evidence to suggest that diet and dietary interventions can have an impact on heart failure (HF) outcomes. Currently, the restriction of salt intake is the only dietary advice that is consistently guideline-recommended for the management of HF despite conflicting evidence for its efficacy. Dietary components that have been investigated in people with HF include middle-chain triglyceride (MCT) oil, beta-hydroxybutyrate (BHB) salts, ketone esters and coenzyme Q10 (CoQ10). Supplementation with these components is thought to be cardioprotective possibly due to an increase in myocardial energy production. There have been research studies on the effectiveness of The Dietary Approaches to Stop Hypertension (DASH) diet and the Mediterranean Diet (MedDiet) in the treatment of HF, but with conflicting results. The ketogenic diet (KD) has come to the forefront of interest due to evidence indicating its effectiveness in addressing the metabolic shift that occurs in HF. However, there is a lack of randomised controlled trials (RCT) centred around the KD. In any dietary intervention, factors such as adherence and compliance affect the validity of the results. Malnutrition, sarcopenia and/or cardiac cachexia can be present in the more advanced stages of heart failure. Nutritional screening, assessment and support/intervention are important aspects of treatment in the advanced stages of heart failure. Furthermore, HF management through dietary intervention is further complicated by the presence of comorbidities, such as diabetes mellitus (DM) and coronary artery disease (CAD). Long-term studies on the use of dietary modifications in people with HF are warranted to ascertain their efficacy, safety and side effects.
Investigation of Delayed Transfusion Reactions in Sickle Cell Disease Patients Polytransfused in the Brazilian Amazon
Authors: Santos Lorena Alves , de Almeida Anne Cristine Gomes , Tarragô Andrea Monteiro , da Silva Nina Rosa Gonçalves , da Silva Juliana Nascimento Vitoriano , de Souza Mônica Moura , Nascimento Monik Oney Oliveira , Nascimento Marcelo Reis do , Castro Ana Caroline dos Santos , de Albuquerque Cinthia Xerez , Cardoso Evilázio Cunha , Neto José Pereira Moura , Albuquerque Sérgio Roberto Lopes
Published: August 2024

Background: Sickle cell disease (SCD) affects approximately 100,000 people in the United States and millions worldwide, with the highest prevalence of 70% of SCD being found in individuals of African ethnicity. Delayed hemolytic, alloimmunization, and anamnestic transfusion reactions in multiple transfusion patients need to be investigated and managed to avoid a worsening of the patient’s clinical status. Objective: This paper aims to investigate delayed transfusion reactions in SCD patients who were polytransfused in the Brazilian Amazon. Material and Methods: The clinical and laboratory indicators of SCD patients with more than four transfusions were investigated. The patients were treated at the Fundação Hospitalar de Hematologia e Hemoterapia do Estado do Amazonas, Brazil. Results: A total of 44 polytransfused patients with SCD were followed. Regarding Rh phenotype, it was possible to observe a frequency of 26.6% (12) patients with the RZRZ (DCE/DCE) phenotype, in addition to 4.5% (two) patients with RH and RHCE variants. It was also possible to observe 20.5% (nine) patients with an alloimmunization reaction, who presented the following alloantibodies: anti-RhD, anti-E, anti-K, anti-Jkb, anti-N, anti-S, and anti-Dia, two of which are unidentified. Of these, four (44.4%) patients also presented autoantibodies, anti-e, and three unidentified antibodies, and four (44.4%) patients presented an anamnestic reaction, with anti-RhD, K, and Jkb antibodies. Of the 44 patients monitored, 54.4% (24) had clinical and laboratory indicators of a delayed hemolytic reaction. Conclusion: Delayed transfusion reactions, often neglected, occur frequently. Therefore, transfusions need to be monitored for at least 28 days, with medical investigation of clinical and laboratory indicators to make greater use of this therapeutic resource.
In Vitro Germination, Micropropagation and Addressing the Hyperhydricity of the Balkan Native Dianthus cruentus, a Plant with High Ornamental and Xeriscaping Potential
Authors: Bazanis Apostolos-Emmanouil , Papafotiou Maria
Published: August 2024

Dianthus cruentus Griseb. (Caryophyllaceae) is an herbaceous perennial native to Greece with a strong ornamental potential when used as a pollinator-friendly component of xeric gardens and green roofs, where it is valued for its tolerance of poor, dry soils, and its showy colorful inflorescences. Aiming to develop an efficient mass propagation protocol appropriate for the introduction of the species as a novel floricultural crop, the in vitro seed and clonal propagation of a Greek native xeric ecotype were investigated in this paper for the first time. A total of 90–100% of the seeds, after being stored in the dark at room temperature for 12 months, germinated when incubated at 10 to 25 °C after their surface sterilization and transfer in vitro. Sixty-day-old seedlings grown in vitro were then used as a source of nodal explants for the initial establishment of micropropagation cultures, more efficiently on MS medium with 0.1 mg L−1 6-benzylaminopurine (BA). In the multiplication stage, either normal or hyperhydric micro-shoots were used as explant sources, assessing the possibility of incorporating usually discarded material in the propagation procedure. Different solid media were tested, with the highest multiplication indices (5.1) recorded in an MS medium containing 0.1 mg L−1 BA and 0.05 mg L−1 NAA, regardless of explants’ hyperhydricity, while an MS medium containing 0.1 mg L−1 BA and 12 g L−1 agar proved optimal for the effective reversal of hyperhydric explants (MI: 5.2). Despite higher hyperhydricity and reaction rates being observed when hyperhydric explants were used, modifications in the multiplication medium proved to be highly effective in controlling hyperhydricity, with the highest number of normal shoots (2.4–2.6) produced in BA-containing media. Micro-shoots rooted readily in ½ MS medium (60–100%), with rooting rates and quality positively affected by the presence of 0.5 mg L−1 IBA in the rooting medium and the absence of cytokinins in the multiplication one. Rooted micro-shoots were successfully acclimatized ex vitro at high rates (65–100%), their origin influencing their acclimatization and morphology. Thus, the concurrent use of normal and hyperhydric shoots in the proposed micropropagation protocol is proven to be both feasible and desirable, as it is able to significantly increase efficiency and facilitate the sustainable exploitation and dissemination of D. cruentus as a promising multivalent horticultural crop.
eHealth and mHealth in Antimicrobial Stewardship to Reduce Mortality in Empirical Antimicrobial Therapy and a Systematic Review with a Meta-Analysis of Adequate Therapy
Authors: Tuon Felipe Francisco , Zequinao Tiago , da Silva Marcelo Silva , Silva Kleber Oliveira
Published: August 2024

The urgent requirement for swift diagnostic methods in pathogen identification and antimicrobial susceptibility testing is emphasized by rising bacterial resistance and limited treatment options, which are particularly critical in sepsis management. The shift from traditional phenotype-based methods to rapid molecular and mass spectrometry techniques has significantly reduced result turnaround times, enhancing patient outcomes. In this systematic review with meta-analysis, the aspects of correct empirical antimicrobial therapy are evaluated to determine their impact on mortality. We performed a systematic review and meta-analysis on EMBASE, the Cochrane Library, Web of Science, and MEDLINE. Studies evaluating mortality associated with empirical adequate and inadequate therapy in different sites of infection were included. Outcomes included clinical cures in microbiologically evaluable patients. Among the sites of infection, the most studied were bloodstream infections (n = 9), followed by respiratory tract infections (n = 5), intra-abdominal infections (n = 5), and urinary tract infections (evaluated by 3 studies). Inadequate therapy was associated with an increase in mortality between 11 and 68%. Technologies to speed up pathogen identification are extremely necessary to reduce mortality.
Aerosol-Mediated Spread of Antibiotic Resistance Genes: Biomonitoring Indoor and Outdoor Environments
Authors: Habibi Nazima , Uddin Saif , Behbehani Montaha , Mustafa Abu Salim , Al-Fouzan Wadha , Al-Sarawi Hanan A. , Safar Hussain , Alatar Fatemah , Al Sawan Rima M. Z.
Published: August 2024

Antimicrobial resistance (AMR) has emerged as a conspicuous global public health threat. The World Health Organization (WHO) has launched the “One-Health” approach, which encourages the assessment of antibiotic resistance genes (ARGs) within an environment to constrain and alleviate the development of AMR. The prolonged use and overuse of antibiotics in treating human and veterinary illnesses, and the inability of wastewater treatment plants to remove them have resulted in elevated concentrations of these metabolites in the surroundings. Microbes residing within these settings acquire resistance under selective pressure and circulate between the air–land interface. Initial evidence on the indoor environments of wastewater treatment plants, hospitals, and livestock-rearing facilities as channels of AMR has been documented. Long- and short-range transport in a downwind direction disseminate aerosols within urban communities. Inhalation of such aerosols poses a considerable occupational and public health risk. The horizontal gene transfer (HGT) is another plausible route of AMR spread. The characterization of ARGs in the atmosphere therefore calls for cutting-edge research. In the present review, we provide a succinct summary of the studies that demonstrated aerosols as a media of AMR transport in the atmosphere, strengthening the need to biomonitor these pernicious pollutants. This review will be a useful resource for environmental researchers, healthcare practitioners, and policymakers to issue related health advisories.
The Long-Term Physical–Psychiatric Comorbidities Related to Childhood Exposure to 9/11 Trauma
Authors: Amsel Lawrence , Cycowicz Yael M. , Rodriguez-Moreno Diana V. , Cheslack-Postava Keely , McReynolds Larkin S. , Musa George J. , Hoven Christina W.
Published: August 2024

Extensive research has explored the enduring effects of childhood trauma on health, revealing its potential to produce chronic health problems. Despite findings that adults exposed to 9/11 suffer from enduring concurrent psychiatric and physical illnesses, investigations into the long-term physical–psychiatric comorbidities experienced by children and adolescents affected by the 9/11 trauma remain limited. In our study, we examined individuals directly exposed to 9/11 as children (N = 844 high exposure and N = 104 low exposed) and compared them to a matched unexposed, control group (N = 491). Fourteen years after their 9/11 exposure, we evaluated their physical and mental health conditions using parent- or youth self-reported health questionnaires, including psychiatric assessments. Those individuals with high 9/11 exposure were significantly more likely to have experienced a psychiatric disorder in the past year and a lifetime physical health condition compared to unexposed individuals. Moreover, the prevalence of physical–psychiatric comorbidities was higher among the 9/11-exposed group, with a 3.5-fold increased prevalence compared to the unexposed group. This underscores how exposure to traumatic events during childhood heightens the risk of long-term concurrent mental and physical health issues. Our findings also highlight the importance of early and ongoing interventions to prevent future comorbidities and promote better quality of life throughout the lifespan.
Exploring Maternal Socio-Demographic Factors Shaping Children’s Dietary Patterns in Brazil: Results from the 2019 National Health Survey
Authors: Brito Flávia dos Santos Barbosa , Costa Emanuela Santos da , Romeiro Ariane Cristina Thoaldo , dos Santos Debora Martins , Brito Alexandre dos Santos , Oliveira Alessandra Silva Dias de , Adegboye Amanda Rodrigues Amorim
Published: August 2024

This study aimed to identify the dietary patterns of Brazilian children aged 6–23 months and to investigate their association with maternal socio-demographic factors. Data from the 2019 Brazilian National Health Survey were used in this cross-sectional study. Mothers of 1616 children aged 6–23 months reported on their children’s dietary intake. Dietary patterns were identified using principal component analysis, and their associations with maternal socio-demographic characteristics were assessed using linear regression models. The first consisted of healthy patterns and the second, unhealthy ones. Linear regression showed that adherence to a healthy dietary pattern was higher among children of mothers who were older (β = 0.02, p = 0.01), had more years of education (β = 0.49, p = 0.04), reported living with a partner (β = 0.29, p = 0.01), and resided in an urban area (β = 0.35, p = 0.01). Conversely, adherence to the unhealthy pattern was positively associated with mothers who declared themselves as black or brown (β = 0.25, p = 0.03). Our results show that older mothers with higher levels of education and paid work and who live with a partner are more likely to contribute to their children’s healthy eating patterns. We conclude that socio-demographic factors may influence the quality of the food offered to children. Nevertheless, advocating for public policies promoting nutritious complementary diets emphasising fresh and minimally processed foods remains crucial for children whose mothers do not possess these favourable socio-demographic characteristics.
Exploring Musculoskeletal Complaints in a Needle Manufacturing Industry: A Cross-Sectional Study
Authors: Anacleto Filho Paulo C. , Braga Ana Cristina , Carneiro Paula
Published: August 2024

Musculoskeletal disorders (MSD) encompass a variety of conditions affecting muscles, joints, and nerves. In Portugal, MSDs are the most prevalent occupational health problem in companies. Based on the relevance of work-related MSD (WMSD), this study aims to assess the prevalence of MSD complaints in a needle manufacturing industry in Northern Portugal, following a cross-sectional approach. Thus, 526 workers from five departments (i.e., operator, tuning, maintenance, administration, and logistics) answered a sociodemographic questionnaire and the Nordic Musculoskeletal Questionnaire (NMQ). Within the last 12 months, females exhibited a higher frequency of complaints than males across all body parts except for ankles/feet. The body parts eliciting the most percentage of complaints for both genders include the lower back (54.2%), neck (42.2%), shoulders (39.0%), ankles/feet (38.2%), and wrists/hands (35.7%). No significant association was found between Body Mass Index (BMI) and body part complaints. Tuners reported the highest complaint rate, with occupations as substantial predictors of complaints in certain body parts. Likewise, complaints tend to increase with age. The findings advocate for ergonomic interventions that are gender-, age-, and job-sensitive.
Adapting the Baseline Resilience Indicators for Communities (BRIC) Framework for England: Development of a Community Resilience Index
Authors: Camacho Christine , Webb Roger T. , Bower Peter , Munford Luke
Published: August 2024

Community resilience results from complex interactions between people, places, and environments. Measuring community resilience aligns with policy objectives to enhance resilience to adverse events and address spatial disparities. The Baseline Resilience Indicators for Communities (BRIC) is a composite index used to measure resilience. This study adapts the BRIC approach to develop a Community Resilience Index (CRI) for England. A systematic review informed indicator selection, and principal components analysis was used to define sub-indices and allocate weightings. The resulting CRI comprised 44 indicators across five domains, quantifying the resilience of English local authorities. Geographical comparisons were made using t-tests and ANOVA, and the CRI was validated against the Index of Multiple Deprivation (IMD). The mean CRI score for local authorities in England was 83.1, ranging from 53.3 to 108.9. Resilience scores showed spatial patterning, with London and the South East scoring highest. The North had lower CRI scores than the Midlands and South (p = 0.022). Coastal and rural areas also showed lower resilience (p < 0.001). CRI and IMD were inversely correlated (r = −0.564, p < 0.0001). This study contributes to the literature by providing the first detailed assessment of community resilience in England using an adapted BRIC framework. The CRI provides a framework for measuring community resilience in England and could be used to explore associations with health outcomes and guide funding allocation.
Overrepresentation of New Workers in Jobs with Multiple Carcinogen Exposures in Canada
Authors: Katende Disann , Rydz Elizabeth , Quinn Emma K. , Heer Emily , Shrestha Raissa , Fazel Sajjad S. , Peters Cheryl E.
Published: August 2024

Background. In Canada, understanding the demographic and job-related factors influencing the prevalence of new workers and their exposure to potential carcinogens is crucial for improving workplace safety and guiding policy interventions. Methods. Logistic regression was performed on the 2017 Labour Force Survey (LFS), to estimate the likelihood of being a new worker based on age, industry, occupation, season, and immigration status. Participants were categorized by sector and occupation using the North American Industry Classification System (NAICS) 2017 Version 1.0 and National Occupational Classification (NOC) system 2016 Version 1.0. Finally, an exposures-per-worker metric was used to highlight the hazardous exposures new workers encounter in their jobs and industries. Results. Individuals younger than 25 years had 3.24 times the odds of being new workers compared to those in the 25–39 age group (adjusted odds ratios (OR) = 3.24, 95% confidence interval (95% CI) = 3.18, 3.31). Recent immigrants (less than 10 years in the country) were more likely to be new workers than those with Canadian citizenship (OR 1.36, 95% CI: 1.32, 1.41). The total workforce exposures-per-worker metric using CAREX Canada data was 0.56. By occupation, new workers were the most overrepresented in jobs in natural resources and agriculture (20.5% new workers), where they also experienced a high exposures-per-worker metric (1.57). Conclusions. Younger workers (under 25 years) and recent immigrants who had arrived 10 or fewer years prior were more likely to be new workers, and were overrepresented in jobs with more frequent hazardous exposures (Construction, Agriculture, and Trades).
The Influence of Proximity on the Evolution of Urban Innovation Networks in Nanjing Metropolitan Area, China: A Comparative Analysis of Knowledge and Technological Innovations
Authors: Shi Yu , Zhai Wei , Yan Yiran , Wang Xingping
Published: August 2024

This study investigates the dynamics of innovation element flows among metropolitan areas and examines the underlying proximity mechanisms that are crucial for elevating urban agglomerations’ innovation levels and spurring their development. Utilizing collaborative publication and patent data, this research constructs knowledge and technological innovation networks within the Nanjing metropolitan area (NMA) from 2013 to 2020. It analyzes the evolution of network structures and applies the Multiple Regression Quadratic Assignment Procedure to discern the proximity mechanisms driving the urban innovation networks’ evolution in NMA. The main findings are as follows: (1) The knowledge collaborations within NMA cities remain largely confined to cities within Jiangsu province, whereas the technological collaborations are shifting from intra-province to cross-province cooperation. (2) Both knowledge and technological innovation networks display a “core-periphery” configuration, with Nanjing maintaining a dominant central position. The scale of the KIN surpasses that of the TIN, while the latter’s growth rate outpaces the former’s. Technological collaborations demonstrate more pronounced spillover effects than their knowledge counterparts. (3) At the metropolitan area level, organizational, social, cognitive, and technological proximities exert varying degrees of influence on innovation cooperation among different innovation entities across various years. Cognitive proximity exhibits the most substantial explanatory power. Based on these findings, the study proposes relevant policy recommendations for constructing an innovative NMA and promoting collaborative innovation development among cities within the NMA.
BS-GeoEduNet 1.0: Blockchain-Assisted Serverless Framework for Geospatial Educational Information Networks
Authors: Kandpal Meenakshi , Goswami Veena , Pritwani Yash , Barik Rabindra K. , Saikia Manob Jyoti
Published: August 2024

The integration of a blockchain-supported serverless computing framework enhances the performance of computational and analytical operations and the provision of services within internet-based data centers, rather than depending on independent desktop computers. Therefore, in the present research paper, a blockchain-assisted serverless framework for geospatial data visualizations is implemented. The proposed BS-GeoEduNet 1.0 framework leverages the capabilities of AWS Lambda for serverless computing, providing a reliable and efficient solution for data storage, analysis, and distribution. The proposed framework incorporates AES encryption, decryption layers, and queue implementation to achieve a scalable approach for handling larger files. It implements a queueing mechanism during the heavier input/output processes of file processing by using Apache KAFKA, enabling the system to handle large volumes of data efficiently. It concludes with the visualization of all geospatial-enabled NIT/IIT details on the proposed framework, which utilizes the data fetched from MongoDB. The experimental findings validate the reliability and efficiency of the proposed system, demonstrating its efficacy in geospatial data storage and processing.
DCPMS: A Large-Scale Raster Layer Serving Method for Custom Online Calculation and Rendering
Authors: Yang Anbang , Zhang Feng , Feng Jie , Wang Luoqi , Yue Enjiang , Fan Xinhua , Zhang Jingyi , Hu Linshu , Wu Sensen
Published: August 2024

Raster data represent one of the fundamental data formats utilized in GIS. As the technology used to observe the Earth continues to evolve, the spatial and temporal resolution of raster data is becoming increasingly refined, while the data scale is expanding. One of the key issues in the development of GIS technology is to determine how to make large-scale raster data better to provide computation, visualization, and analysis services in the Internet environment. This paper proposes a decentralized COG-pyramid-based map service method (DCPMS). In comparison to traditional raster data online service technology, such as GIS servers and static tiles, DCPMS employs virtual mapping to reduce data storage costs and combines tile technology with a cloud-native storage scheme to enhance the concurrency of supportable requests. Furthermore, the band calculation process is shifted to the client, thereby effectively resolving the issue of efficient customized band calculation and data rendering in the context of a large-scale raster data online service. The results indicate DCPMS delivers commendable performance. Its decentralized architecture significantly enhances performance in high concurrency scenarios. With a thousand concurrent requests, the response time of DCPMS is reduced by 74% compared to the GIS server. Moreover, this service exhibits considerable strengths in data preprocessing and storage, suggesting a novel pathway for future technical improvement of large-scale raster data map services.
Concurrent Analysis of Tiafenacil and Its Transformation Products in Soil by Using Newly Developed UHPLC-QTOF-MS/MS-Based Approaches
Authors: Zhou Wenwen , Yan Anqi , Zhang Shujie , Peng Dayong , Li Jun
Published: August 2024

As new pesticides continue to emerge in agricultural systems, understanding their environmental behavior is crucial for effective risk assessment. Tiafenacil (TFA), a promising novel pyrimidinedione herbicide, was the focus of this study. We developed an efficient QuEChERS-UHPLC-QTOF-MS/MS method to measure TFA and its transformation products (TP1, TP2, TP3, TP4, and TP5) in soil. Our calibration curves exhibited strong linearity (R2 ≥ 0.9949) ranging from 0.015 to 2.0 mg/kg within a low limit of quantification (LOQ) of 2.0 µg/kg. Inter-day and intra-day recoveries (0.10 to 2.0 mg/kg, 80.59% to 110.05%, RSD from 0.28% to 12.93%) demonstrated high sensitivity and accuracy. Additionally, TFA dissipation under aerobic conditions followed first-order kinetics, mainly yielding TP1 and TP4. In contrast, TP1 and TP2 were mainly found under sterilized and anaerobic conditions, and TFA dissipation followed second-order kinetics. Moreover, we predicted the transformation pathways of TFA using density functional theory (DFT) and assessed the toxicity levels of TFA and its TPs to aquatic organisms using ECOSAR. Collectively, these findings hold significant implications for a better understanding of TFA fate in diversified soil, benefiting its risk assessment and rational utilization.
Anti-HER2 Cancer-Specific mAb, H2Mab-250-hG1, Possesses Higher Complement-Dependent Cytotoxicity than Trastuzumab
Authors: Suzuki Hiroyuki , Ohishi Tomokazu , Tanaka Tomohiro , Kaneko Mika K. , Kato Yukinari
Published: August 2024

Cancer-specific monoclonal antibodies (CasMabs) that recognize cancer-specific antigens with in vivo antitumor efficacy are innovative therapeutic strategies for minimizing adverse effects. We previously established a cancer-specific anti-human epidermal growth factor receptor 2 (HER2) monoclonal antibody (mAb), H2Mab-250/H2CasMab-2. In flow cytometry and immunohistochemistry, H2Mab-250 reacted with HER2-positive breast cancer cells but did not show reactivity to normal epithelial cells. In contrast, a clinically approved anti-HER2 mAb, trastuzumab, strongly recognizes both breast cancer and normal epithelial cells in flow cytometry. The human IgG1 version of H2Mab-250 (H2Mab-250-hG1) possesses compatible in vivo antitumor effects against breast cancer xenografts to trastuzumab despite the lower affinity and effector activation than trastuzumab in vitro. This study compared the antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cellular cytotoxicity (CDC) between H2Mab-250-hG1 and trastuzumab. Both H2Mab-250-hG1 and trastuzumab showed ADCC activity against HER2-overexpressed Chinese hamster ovary -K1 and breast cancer cell lines (BT-474 and SK-BR-3) in the presence of human natural killer cells. Some tendency was observed where trastuzumab showed a more significant ADCC effect compared to H2Mab-250-hG1. Importantly, H2Mab-250-hG1 exhibited superior CDC activity in these cells compared to trastuzumab. Similar results were obtained in the mouse IgG2a types of both H2Mab-250 and trastuzumab. These results suggest the different contributions of ADCC and CDC activities to the antitumor effects of H2Mab-250-hG1 and trastuzumab, and indicate a future direction for the clinical development of H2Mab-250-hG1 against HER2-positive tumors.
Senescence in Adipose-Derived Stem Cells: Biological Mechanisms and Therapeutic Challenges
Authors: Foti Riccardo , Storti Gabriele , Palmesano Marco , Scioli Maria Giovanna , Fiorelli Elena , Terriaca Sonia , Cervelli Giulio , Kim Bong Sung , Orlandi Augusto , Cervelli Valerio
Published: August 2024

Adipose tissue-derived stem cells (ADSCs) represent a subset of the mesenchymal stem cells in every adipose compartment throughout the body. ADSCs can differentiate into various cell types, including chondrocytes, osteocytes, myocytes, and adipocytes. Moreover, they exhibit a notable potential to differentiate in vitro into cells from other germinal lineages, including endothelial cells and neurons. ADSCs have a wide range of clinical applications, from breast surgery to chronic wounds. Furthermore, they are a promising cell population for future tissue-engineering uses. Accumulating evidence indicates a decreased proliferation and differentiation potential of ADSCs with an increasing age, increasing body mass index, diabetes mellitus, metabolic syndrome, or exposure to radiotherapy. Therefore, the recent literature thoroughly investigates this cell population’s senescence mechanisms and how they can hinder its possible therapeutic applications. This review will discuss the biological mechanisms and the physio-pathological causes behind ADSC senescence and how they can impact cellular functionality. Moreover, we will examine the possible strategies to invert these processes, re-establishing the full regenerative potential of this progenitor population.