bluuebunny/crossref_metadata_2025 huggingface dataset:
Mitral valve perforation after left lateral accessory pathway ablation: a case report
Authors: Jabeur Mariem , Carabelli Adrien , Jacon Peggy , Venier Sandrine , Obadia Jean-François , Defaye Pascal
Published: March 2022

Background

Radiofrequency catheter ablation is considered to be a relatively safe procedure. This is an unusual case report in which severe mitral regurgitation was occurred after left lateral accessory pathway radiofrequency catheter ablation.

Case presentation

A 15-year-old man without structural heart disease was referred for ablation of a left lateral accessory pathway. He was a rugby player who had lived with Wolff–Parkinson–White syndrome since 2017. In 2017, two failed extensive radiofrequency catheter ablations of a left lateral accessory pathway had been performed in another center. In June 2018, he underwent a third radiofrequency catheter ablation of a left lateral accessory pathway using an anterograde transseptal approach with an early recurrence one month later. A successful fourth procedure was performed in August 2018 using a retrograde aortic approach. Three months later, the patient presented to the hospital with atypical chest pain and dyspnea on exertion. Transthoracic echocardiography revealed severe mitral regurgitation caused by a perforation of the posterior leaflet. Given the symptoms and the severity of the mitral valve regurgitation, the decision was taken to proceed with surgical intervention. Posterior mitral leaflet perforation was confirmed intraoperatively. The patient underwent video-assisted mitral valve repair via Minithoracotomy approach.

Conclusion

This case demonstrates a very rare complication of Wolff–Parkinson–White radiofrequency ablation.
Genetic susceptibility to hereditary non-medullary thyroid cancer
Authors: Kamani Tina , Charkhchi Parsa , Zahedi Afshan , Akbari Mohammad R.
Published: March 2022

Non-medullary thyroid cancer (NMTC) is the most common type of thyroid cancer. With the increasing incidence of NMTC in recent years, the familial form of the disease has also become more common than previously reported, accounting for 5–15% of NMTC cases. Familial NMTC is further classified as non-syndromic and the less common syndromic FNMTC. Although syndromic NMTC has well-known genetic risk factors, the gene(s) responsible for the vast majority of non-syndromic FNMTC cases are yet to be identified. To date, several candidate genes have been identified as susceptibility genes in hereditary NMTC. This review summarizes genetic predisposition to non-medullary thyroid cancer and expands on the role of genetic variants in thyroid cancer tumorigenesis and the level of penetrance of NMTC-susceptibility genes.
The spinach YY genome reveals sex chromosome evolution, domestication, and introgression history of the species
Authors: Ma Xiaokai , Yu Li’ang , Fatima Mahpara , Wadlington William H. , Hulse-Kemp Amanda M. , Zhang Xingtan , Zhang Shengcheng , Xu Xindan , Wang Jingjing , Huang Huaxing , Lin Jing , Deng Ban , Liao Zhenyang , Yang Zhenhui , Ma Yanhong , Tang Haibao , Van Deynze Allen , Ming Ray
Published: March 2022

Background

Spinach (Spinacia oleracea L.) is a dioecious species with an XY sex chromosome system, but its Y chromosome has not been fully characterized. Our knowledge about the history of its domestication and improvement remains limited.

Results

A high-quality YY genome of spinach is assembled into 952 Mb in six pseudo-chromosomes. By a combination of genetic mapping, Genome-Wide Association Studies, and genomic analysis, we characterize a 17.42-Mb sex determination region (SDR) on chromosome 1. The sex chromosomes of spinach evolved when an insertion containing sex determination genes occurred, followed by a large genomic inversion about 1.98 Mya. A subsequent burst of SDR-specific repeats (0.1–0.15 Mya) explains the large size of this SDR. We identify a Y-specific gene, NRT1/PTR 6.4 which resides in this insertion, as a strong candidate for the sex determination or differentiation factor. Resequencing of 112 spinach genomes reveals a severe domestication bottleneck approximately 10.87 Kya, which dates the domestication of spinach 7000 years earlier than the archeological record. We demonstrate that a strong selection signal associated with internode elongation and leaf area expansion is associated with domestication of edibility traits in spinach. We find that several strong genomic introgressions from the wild species Spinacia turkestanica and Spinacia tetrandra harbor desirable alleles of genes related to downy mildew resistance, frost resistance, leaf morphology, and flowering-time shift, which likely contribute to spinach improvement.

Conclusions

Analysis of the YY genome uncovers evolutionary forces shaping nascent sex chromosome evolution in spinach. Our findings provide novel insights about the domestication and improvement of spinach.
Single-cell transcriptomics of popliteal lymphatic vessels and peripheral veins reveals altered lymphatic muscle and immune cell populations in the TNF-Tg arthritis model
Authors: Kenney H. Mark , Wu Chia-Lung , Loiselle Alayna E. , Xing Lianping , Ritchlin Christopher T. , Schwarz Edward M.
Published: March 2022

Background

Lymphatic dysfunction exists in tumor necrosis factor transgenic (TNF-Tg) mice and rheumatoid arthritis (RA) patients. While joint-draining TNF-Tg popliteal lymphatic vessels (PLVs) have deficits in contractility during end-stage arthritis, the nature of lymphatic muscle cells (LMCs) and their TNF-altered transcriptome remain unknown. Thus, we performed single-cell RNA-sequencing (scRNAseq) on TNF-Tg LMCs in PLVs efferent to inflamed joints versus wild-type (WT) controls.

Methods

Single-cell suspensions of PLVs were sorted for smooth muscle cells (SMCs), which was validated by Cspg4-Cre;tdTomato reporter gene expression. Single-cell RNA-seq was performed on a 10x Genomics platform and analyzed using the Seurat R package. Uniform Manifold Approximation and Projections (UMAPs) and Ingenuity Pathway Analysis software were used to assess cell clusters and functional genomics in WT vs. TNF-Tg populations.

Results

Fluorescent imaging of Cspg4-Cre;tdTomato vessels demonstrated dim PLVs and strong reporter gene expression in the adjacent superficial saphenous vein, which was corroborated by flow cytometry of LMCs and vascular smooth muscle cells (VSMCs) from these vessels. Due to their unique morphology, these populations could also be readily detected by scatter analysis of cells from non-fluorescent mice. Bioinformatics analysis of flow sorted WT and TNF-Tg cells identified 20 unique cell clusters that together were 22.4% LMCs, 15.0% VSMCs, and 62.6% non-muscle cells of 8879 total cells. LMCs and M2-macrophages were decreased, while inflammatory monocytes were increased in TNF-Tg lower limb vasculature. SMC populations were defined by Cald1, Tpm1, and Pdgfrb expression and were enriched in myofibroblast-like gene expression. TNF-Tg LMCs exhibited enhanced functional genomics associated with cell death, phagocyte recruitment, and joint inflammation. Among the most prominent TNF-induced genes in SMCs were Mmp3, Cxcl12, and Ccl19, and the most downregulated genes were Zbtb16, Galnt15, and Apod.

Conclusions

Single-cell RNA-seq can be used to investigate functional genomics of lower limb vasculature in mice. Our findings confirm the inflammatory transcriptome of TNF-Tg vessels and altered gene expression in SMC populations. This study further supports a potential role of mesenchymal stromal cells in inflammatory-erosive arthritis pathogenesis, and warrants future studies to define the effects of this TNF-altered transcriptome on PLV function and joint homeostasis.
Using ZINC08918027 inhibitor to determine Aurora kinase-chromosomal passenger complex isoforms in mouse oocytes
Authors: Kratka Caroline , Drutovic David , Blengini Cecilia S. , Schindler Karen
Published: March 2022

Objective

Miscarriages affect 10% of women aged 25–29, and 53% of women over 45. The primary cause of miscarriage is aneuploidy that originated in eggs. The Aurora kinase family has three members that regulate chromosome segregation. Therefore, distinguishing the roles of these isoforms is important to understand aneuploidy etiology. In meiosis, Aurora kinase A (AURKA) localizes to spindle poles, where it binds TPX2. Aurora kinase C (AURKC) localizes on chromosomes, where it replaces AURKB as the primary AURK in the chromosomal passenger complex (CPC) via INCENP binding. Although AURKA compensates for CPC function in oocytes lacking AURKB/C, it is unknown whether AURKA binds INCENP in wild type mouse oocytes. ZINC08918027 (ZC) is an inhibitor that prevents the interaction between AURKB and INCENP in mitotic cells. We hypothesized that ZC would block CPC function of any AURK isoform.

Results

ZC treatment caused defects in meiotic progression and spindle building. By Western blotting and immunofluorescence, we observed that activated AURKA and AURKC levels in ZC-treated oocytes decreased compared to controls. These results suggest there is a population of AURKA-CPC in mouse oocytes. These data together suggest that INCENP-dependent AURKA and AURKC activities are needed for spindle bipolarity and meiotic progression.
Preliminary report on harmonization of features extraction process using the ComBat tool in the multi-center “Blue Sky Radiomics” study on stage III unresectable NSCLC
Authors: Cabini Raffaella Fiamma , Brero Francesca , Lancia Andrea , Stelitano Chiara , Oneta Olga , Ballante Elena , Puppo Emanuela , Mariani Manuel , Alì Emanuele , Bartolomeo Valentina , Montesano Marianna , Merizzoli Elisa , Aluia Diana , Agustoni Francesco , Stella Giulia Maria , Sun Roger , Bianchini Linda , Deutsch Eric , Figini Silvia , Bortolotto Chandra , Preda Lorenzo , Lascialfari Alessandro , Filippi Andrea Riccardo
Published: March 2022

Background and purpose

In the retrospective-prospective multi-center "Blue Sky Radiomics” study (NCT04364776), we plan to test a pre-defined radiomic signature in a series of stage III unresectable NSCLC patients undergoing chemoradiotherapy and maintenance immunotherapy. As a necessary preliminary step, we explore the influence of different image-acquisition parameters on radiomic features’ reproducibility and apply methods for harmonization.

Material and methods

We identified the primary lung tumor on two computed tomography (CT) series for each patient, acquired before and after chemoradiation with i.v. contrast medium and with different scanners. Tumor segmentation was performed by two oncological imaging specialists (thoracic radiologist and radio-oncologist) using the Oncentra Masterplan® software. We extracted 42 radiomic features from the specific ROIs (LIFEx). To assess the impact of different acquisition parameters on features extraction, we used the Combat tool with nonparametric adjustment and the longitudinal version (LongComBat).

Results

We defined 14 CT acquisition protocols for the harmonization process. Before harmonization, 76% of the features were significantly influenced by these protocols. After, all extracted features resulted in being independent of the acquisition parameters. In contrast, 5% of the LongComBat harmonized features still depended on acquisition protocols.

Conclusions

We reduced the impact of different CT acquisition protocols on radiomic features extraction in a group of patients enrolled in a radiomic study on stage III NSCLC. The harmonization process appears essential for the quality of radiomic data and for their reproducibility.

ClinicalTrials.gov Identifier: NCT04364776, First Posted:April 28, 2020, Actual Study Start Date: April 15, 2020, https://clinicaltrials.gov/ct2/show/NCT04364776.
Hyperammonemia of unknown cause in a young postpartum woman: a case report
Authors: Hanif Sadaf , Sethi Sher Muhammad
Published: March 2022

Background

Hyperammonemia is a medical condition described as increased or elevated serum ammonia levels. High serum levels of ammonia can cause neurotoxicity. Sudden onset severe hyperammonemia may cause severe encephalopathy with brain damage. It can result in cerebral edema, emesis, seizures, hypotonia, and death. We report a young postpartum woman who had a sudden rise in serum ammonia levels after vaginal delivery.

Case presentation

A 24-year-old, married, postpartum Pakistani woman was admitted to the intensive care unit through the emergency department, with complaints of fever, severe abdominal pain with distension, and altered levels of consciousness. The patient had a medical history of spontaneous vaginal delivery 2 weeks before this hospital admission, after which she gradually developed the above symptoms. However, the patient’s past medical history was unremarkable with no hepatic disease, but her investigations revealed a progressive rise in serum ammonia levels. In the intensive care unit, she developed generalized tonic–clonic seizures. This was followed by a coma, tonsillar herniation, and death.

Conclusion

Postpartum hyperammonemia is a rare entity. It is a critical illness and must be evaluated for underlying metabolic disorders. Early diagnosis and treatment may result in better outcomes and reduced mortality among postpartum women with hyperammonemia.
Increased phosphatase regenerating liver-1 trigger vascular remodeling in injured ovary via platelet-derived growth factor signaling pathway
Authors: Park Hyeri , Seok Jin , You Jun Hyeong , Kim Jae Yeon , Lim Ja-Yun , Kim Gi Jin
Published: March 2022

Background

Vascular abnormalities in the ovary cause infertility accompanied by ovarian insufficiency due to a microenvironment of barren ovarian tissues. Placenta-derived mesenchymal stem cells (PD-MSCs, Naïve) treatment in ovarian dysfunction shows angiogenic effect, however, the therapeutic mechanism between ovarian function and vascular remodeling still unclear. Therefore, we examined whether by phosphatase regenerating liver-1 (PRL-1), which is correlated with angiogenesis in reproductive systems, overexpressed PD-MSCs could maximize the angiogenic effects in an ovarian tissues injured of rat model with partial ovariectomy and their therapeutic mechanism by enhanced vascular function via PDGF signaling.

Methods

PD-MSCsPRL-1 (PRL-1) were generated by nonviral AMAXA gene delivery system and analyzed the vascular remodeling and follicular development in ovary. One week after Sprague–Dawley (SD) rats ovariectomy, Naïve and PRL-1 was transplanted. The animals were sacrificed at 1, 3 and 5 weeks after transplantation and vascular remodeling and follicular development were analyzed. Also, human umbilical vein endothelial cells (HUVECs) and ovarian explantation culture were performed to prove the specific effects and mechanism of PRL-1.

Results

Vascular structures in ovarian tissues (e.g., number of vessels, thickness and lumen area) showed changes in the Naïve and PRL-1-overexpressed PD-MSC (PRL-1) transplantation (Tx) groups compared to the nontransplantation (NTx) group. Especially, PRL-1 induce to increase the expression of platelet-derived growth factor (PDGF), which plays a role in vascular remodeling as well as follicular development, compared to the NTx. Also, the expression of genes related to pericyte and vascular permeability in arteries was significantly enhanced in the PRL-1 compared to the NTx (p < 0.05). PRL-1 enhanced the vascular formation and permeability of human umbilical vein endothelial cells (HUVECs) via activated the PDGF signaling pathway.

Conclusions

Our results show that PRL-1 restored ovarian function by enhanced vascular function via PDGF signaling pathway. These findings offer new insight into the effects of functionally enhanced stem cell therapy for reproductive systems and should provide new avenues to develop more efficient therapies in degenerative medicine.
Decoding ceRNA regulatory network in the pulmonary artery of hypoxia-induced pulmonary hypertension (HPH) rat model
Authors: Wang Jun , Niu Yanqin , Luo Lingjie , Lu Zefeng , Chen Qinghua , Zhang Shasha , Guo Qianwen , Li Li , Gou Deming
Published: March 2022

Background

Hypoxia-induced pulmonary hypertension (HPH) is a lethal cardiovascular disease with the characteristic of severe remodeling of pulmonary vascular. Although a large number of dysregulated mRNAs, lncRNAs, circRNAs, and miRNAs related to HPH have been identified from extensive studies, the competitive endogenous RNA (ceRNA) regulatory network in the pulmonary artery that responds to hypoxia remains largely unknown.

Results

Transcriptomic profiles in the pulmonary arteries of HPH rats were characterized through high-throughput RNA sequencing in this study. Through relatively strict screening, a set of differentially expressed RNAs (DERNAs) including 19 DEmRNAs, 8 DElncRNAs, 19 DEcircRNAs, and 23 DEmiRNAs were identified between HPH and normal rats. The DEmRNAs were further found to be involved in cell adhesion, axon guidance, PPAR signaling pathway, and calcium signaling pathway, suggesting their crucial role in HPH. Moreover, a hypoxia-induced ceRNA regulatory network in the pulmonary arteries of HPH rats was constructed according to the ceRNA hypothesis. More specifically, the ceRNA network was composed of 10 miRNAs as hub nodes, which might be sponged by 6 circRNAs and 7 lncRNAs, and directed the expression of 18 downstream target genes that might play important role in the progression of HPH. The expression patterns of selected DERNAs in the ceRNA network were then validated to be consistent with sequencing results in another three independent batches of HPH and normal control rats. The diagnostic effectiveness of several hub mRNAs in ceRNA network was further evaluated through investigating their expression profiles in patients with pulmonary artery hypertension (PAH) recorded in the Gene Expression Omnibus (GEO) dataset GSE117261. Dysregulated POSTN, LTBP2, SPP1, and LSAMP were observed in both the pulmonary arteries of HPH rats and lung tissues of PAH patients.

Conclusions

A ceRNA regulatory network in the pulmonary arteries of HPH rats was constructed, 10 hub miRNAs and their corresponding interacting lncRNAs, circRNAs, and mRNAs were identified. The expression patterns of selected DERNAs were further validated to be consistent with the sequencing result. POSTN, LTBP2, SPP1, and LSAMP were suggested to be potential diagnostic biomarkers and therapeutic targets for PAH.
Linc-RAM promotes muscle cell differentiation via regulating glycogen phosphorylase activity
Authors: Zhai Lili , Wan Xin , Wu Rimao , Yu Xiaohua , Li Hu , Zhong Ran , Zhu Dahai , Zhang Yong
Published: March 2022

Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes, especially skeletal muscle cell differentiation. Most of the lncRNAs identified to date are localized in the nucleus and play regulatory roles in gene expression. The cytoplasmic lncRNAs are less well understood. We previously identified a long intergenic non-coding RNA (linc-RNA) activator of myogenesis (Linc-RAM) that directly binds MyoD in the nucleus to enhance muscle cell differentiation. Here, we report that a substantial fraction of Linc-RAM is localized in the cytoplasm of muscle cells. To explore the molecular functions of cytoplasmic Linc-RAM, we sought to identify Linc-RAM-binding proteins. We report here that Linc-RAM physically interacts with glycogen phosphorylase (PYGM) in the cytoplasm. Knockdown of PYGM significantly attenuates the function of Linc-RAM in promoting muscle cell differentiation. Loss-of-function and gain-of function assays demonstrated that PYGM enhances muscle cell differentiation in an enzymatic activity-dependent manner. Finally, we show that the interaction between Linc-RAM and PYGM positively regulates the enzymatic activity of PYGM in muscle cells. Collectively, our findings unveil a molecular mechanism through which cytoplasmic Linc-RAM contributes to muscle cell differentiation by regulating PYGM activity. Our findings establish that there is crosstalk between lncRNAs and cellular metabolism during myogenic cell differentiation.
Research on test and evaluation method of laser wireless power transmission system
Authors: Xu Wanli , Wang Xudong , Li Weishi , Li Shizhan , Lu Changbo
Published: March 2022

Laser wireless power transmission technology avoids the traditional cable transmission requirements regarding the territory and environment, and facilitates vehicle-mounted laser wireless power transmission to provide accompanying power supplies to multi-target unmanned clusters. This can reduce the dependence of unmanned equipment (especially unmanned aerial vehicles) on power storage batteries, providing broad application prospects for the battlefield. However, the current laser wireless power transmission technology lacks a standardized technical index system and mature test and evaluation method; this is not conducive to the application of this technology in diverse combat scenarios. Accordingly, this paper proposes specific indicators for the component-level and system-level performance testing of laser wireless power transmission technology and constructs a corresponding test system and evaluation method, providing a yardstick for selecting laser wireless power transmission systems adapted to the battlefield.
Iterative algorithm for approximating fixed points of multivalued quasinonexpansive mappings in Banach spaces
Authors: Minjibir Ma’aruf Shehu , Izuazu Chimezie
Published: March 2022

Let E be a strictly convex real Banach space and let $D\subseteq E$
D
⊆
E
be a nonempty closed convex subset of E. Let $T_{i}: {D}\longrightarrow \mathcal{P}({D})$

T
i

:
D
⟶
P
(
D
)
, $i=1,2,3,\ldots $
i
=
1
,
2
,
3
,
…
be a countable family of quasinonexpansive multivalued maps that are continuous with respect to the Hausdorff metric, $\mathcal{P}(D)$
P
(
D
)
is the family of proximinal and bounded subsets of D. Supposing that the family has at least one common fixed point, we show that a Krasnoselskii–Mann-type sequence converges strongly to a common fixed point. Our result generalizes and complements some important results for single-valued and multivalued quasinonexpansive maps.
The Public Health 12 framework: interpreting the ‘Meadows 12 places to act in a system’ for use in public health
Authors: Bolton Kristy A. , Whelan Jillian , Fraser Penny , Bell Colin , Allender Steven , Brown Andrew D.
Published: March 2022

Background

Systems science approaches have demonstrated effectiveness in identifying underlying drivers of complex problems and facilitating the emergence of potential interventions that are locally tailored, feasible, sustainable and evidence informed. Despite the potential usefulness of system dynamics simulation modelling and other systems science modelling techniques in guiding implementation, time and cost constraints have limited its ability to provide strong guidance on how to implement complex interventions in communities. Guidance is required to ensure systems interventions lead to impactful systems solutions, implemented utilising strategies from the intersecting fields of systems science and implementation science. To provide cost-effective guidance on how and where to implement in systems, we offer a translation of the ‘Meadows 12 places to act in a system’ (Meadows 12) into language useful for public health.

Methods

This translation of Meadows 12 was informed by our experience in working with 31 communities across two complex large scale randomised control trials and one large whole of community case study. These research projects utilised systems science and implementation science to co-create childhood obesity prevention interventions. The team undertaking this translation comprised research academics, implementation specialists and practitioners, practice-based researchers and a systems dynamicist. Our translation of each of the Meadows 12 levels to act in the system maintains the fidelity and nuance of the 12 distinct levels. We provide examples of each level of the Public Health 12 framework (PH12) drawn from 31 communities. All research was conducted in Victoria, Australia between 2016 and 2020.

Results

PH12 provides a framework to guide both research and practice in real world contexts to implement targeted system level interventions. PH12 can be used with existing implementation science theory to identify relevant strategies for implementation of these interventions to impact the system at each of the leverage points.

Conclusion

To date little guidance for public health practitioners and researchers exists regarding how to implement systems change in community-led public health interventions. PH12 enables operationalisation Meadows 12 systems theory into public health interventions. PH12 can help research and practice determine where leverage can be applied in the system to optimise public health systems level interventions and identify gaps in existing efforts.

Trial registration

WHO STOPS: ANZCTR: 12616000980437. RESPOND: ANZCTR: 12618001986268p.
Influence of land cover features on avian community and potential conservation priority areas for biodiversity at a Ramsar site in India
Authors: Barik Souvik , Saha Goutam Kumar , Mazumdar Subhendu
Published: March 2022

Background

Wetlands provide diverse ecological services to sustain rich biodiversity, but they are recklessly exploited around the globe. East Kolkata Wetlands (EKW; Ramsar site No. 1208, ~ 125 km2) was once very rich in faunal diversity that declined over the years due to various anthropogenic pressures. In such scenarios, effective management plans need to contemplate local and landscape level initiatives and prioritize areas for conservation. Studying the foraging guild structure and spatial patterns of relationship between bird and its environment provides important insight of ecological processes of avifauna. Thus, we carried out extensive surveys in 142 point count stations to explore the diversity and foraging guild structure of birds in EKW. We addressed two important questions that how bird diversity varied from the outer periphery of the city towards the wetlands and how bird diversity is influenced by land cover features at local and landscape level in wetland complex. The goal of this study was to prepare a spatial map of avian diversity and to find the conservation priority areas for birds in this internationally recognized wetland complex.

Results

We recorded 94 species of birds and the Shannon diversity index values ranged between 1.249 and 3.189. We found that the avian diversity increased with increasing distance from city periphery. Omnivores and insectivores were the dominant foraging guilds, followed by carnivores, granivores, frugivores and piscivores. Generalized Linear Models revealed that bird communities were positively influenced by areas of wetlands, dikes and banks, agricultural field and emergent vegetation, and negatively influenced by built-up area. In the projected spatial map of species diversity, 74.15 km2area has high diversity. Finally, we identified 12.82 km2of top 10% conservation priority areas.

Conclusions

Low bird diversity near city periphery is related to habitat degradation due to anthropogenic pressure. Dominance of omnivores and insectivores indicates a possible impact of urbanization in EKW. We suggest that a mix of agricultural field, wetland areas and emergent vegetation helps to sustain a greater avian community. Besides, protection of conservation priority areas, restoration of the degraded habitats and reduction of threats (particularly in the north-eastern parts of EKW near the city periphery) will ensure the conservation of avifauna, and also the overall biodiversity of EKW.
Six-month outcomes of the HOPE smartphone application designed to support treatment with medications for opioid use disorder and piloted during an early statewide COVID-19 lockdown
Authors: Hodges Jacqueline , Waselewski Marika , Harrington William , Franklin Taylor , Schorling Kelly , Huynh Jacqueline , Tabackman Alexa , Otero Kori , Ingersoll Karen , Tiouririne Nassima Ait-Daoud , Flickinger Tabor , Dillingham Rebecca
Published: March 2022

Background

Morbidity and mortality related to opioid use disorder (OUD) in the U.S. is at an all-time high. Innovative approaches are needed to address gaps in retention in treatment with medications for opioid use disorder (MOUD). Mobile health (mHealth) approaches have shown improvement in engagement in care and associated clinical outcomes for a variety of chronic diseases, but mHealth tools designed specifically to support patients treated with MOUD are limited.

Methods

Following user-centered development and testing phases, a multi-feature smartphone application called HOPE (Heal. Overcome. Persist. Endure) was piloted in a small cohort of patients receiving MOUD and at high risk of disengagement in care at an office-based opioid treatment (OBOT) clinic in Central Virginia. Outcomes were tracked over a six-month period following patient enrollment. They included retention in care at the OBOT clinic, usage of various features of the application, and self-rated measures of mental health, substance use, treatment and recovery.

Results

Of the 25 participants in the HOPE pilot study, a majority were retained in care at 6 months (56%). Uptake of bi-directional features including messaging with providers and daily check-ins of mood, stress and medication adherence peaked at one month, and usage persisted through the sixth month. Patients who reported that distance to clinic was a problem at baseline had higher loss to follow up compared to those without distance as a reported barrier (67% vs 23%, p = 0.03). Patients lost to in-person clinic follow up continued to engage with one or more app features, indicating that mHealth approaches may bridge barriers to clinic visit attendance. Participants surveyed at baseline and 6 months (N = 16) scored higher on scales related to overall self-control and self-efficacy related to drug abstinence.

Conclusions

A pilot study of a novel multi-feature smartphone application to support OUD treatment showed acceptable retention in care and patient usage at 6 months. Further study within a larger population is needed to characterize ‘real world’ uptake and association with outcomes related to retention in care, relapse prevention, and opioid-associated mortality.
On-site communication measures as a tool in outdoor recreation management: a systematic map protocol
Authors: Selvaag Sofie Kjendlie , Keller Rose , Aas Øystein , Gundersen Vegard , Singsaas Frode Thomassen
Published: March 2022

Background

Communication is a central tool in managing the balance between outdoor recreation and environmental protection. Several studies have evaluated different communication measures in nature area case studies, but rarely are these measures compared across contexts. We systematically map the literature guided by the question, what is the evidence base of on-site communication in outdoor recreation to change human behavior towards a more sustainable direction? Taking vulnerable natural areas as our starting point, we map distribution and abundance of communication measures, study design and outcome-related themes.

Methods

The target population for our mapping review (hereafter review) are outdoor recreationists and nature-based tourists who visit natural or near-natural settings. We will examine the studies that have crafted written, oral and visual intervention measures to change behavior by using persuasion, education and information instead of legal restrictions or bans. Some examples of challenges addressed with communication measures are proper waste disposal, using designated trails, minimizing wear and tear at campsites, avoid disturbing wildlife, and encouraging appropriate and safe behavior. No geographic restrictions will be applied but we will focus on protected areas. We will search publication databases for peer-reviewed published articles using internet and specialist searches to identify grey literature in English. We will screen first by title, followed by abstract and finally full text. For each article selected for full-text screening, metadata will be extracted on key variables of interest.

The extracted data from the coding will be used to group and compare the studies to reveal knowledge gaps and knowledge clusters. We will briefly describe findings from the included studies. The review will help identify what type of human behavior researchers have addressed with communication in nature management and conservation. In addition it will highlight which communication measures are frequently used in each behavioral context. It will identify which frameworks and communication theories have been the basis for designing intervention measures and provide support to practitioners and researchers in future framing and implementation of communication measures in natural settings.
Effects of arm and leg positions on lumbar multifidus muscle activity while on hands and knees or while standing
Authors: Mitani Yasuhiro , Hanafusa Masaru , Hashimoto Junichi , Inada Ryuta , Koda Hitoshi
Published: March 2022

Background

The lumbar multifidus (LMF) muscle, which is involved in the mechanical stability of the lumbar spine, reportedly undergoes atrophy in patients with low back pain. Preventing or mitigating low back pain requires strengthening the LMF muscle; however, methods for triggering selective and significant contraction of this muscle have not been fully studied. This study aims to clarify how, in the hands-and-knees or standing position, the position of the arm and leg on one side affects the activity of the lumbar erector spinae (LES) and LMF muscles.

Methods

We recruited nine adult men with no prior history of low back pain. Measurements were taken in four different postures under varying conditions (that is, one arm and one leg were lifted in either the hands-and-knees or standing position,) as follows: (1) shoulder joint flexion and hip joint extension in the hands-and-knees position; (2) 90° shoulder joint abduction and hip joint abduction in the hands-and-knees position; (3) shoulder joint flexion and hip joint extension in the standing position; and (4) 90° shoulder joint abduction and hip joint abduction in the standing position. The 90° shoulder joint abduction involved simultaneous horizontal abduction, while the hip joint abduction involved simultaneous extension. Muscle activity of the LES and LMF in each posture was measured using a surface electromyograph.

Results

Muscle activity of the LMF was significantly higher in 90° shoulder joint abduction and hip joint abduction than in shoulder joint flexion and hip joint extension in both the hands-and-knees and standing positions. The LES muscle showed no significant differences in activity between each posture.

Conclusions

The results suggest that unilateral 90° shoulder joint abduction and contralateral hip joint abduction in the hands-and-knees and standing positions may produce selective and significant contraction of the LMF muscle.
Homocysteine as a potential indicator of endothelial dysfunction and cardiovascular risk in female patients with borderline personality disorder
Authors: Kern Katharina , Sinningen Kathrin , Engemann Luisa , Maiß Clara , Hanusch Beatrice , Mügge Andreas , Lücke Thomas , Brüne Martin
Published: March 2022

Background

There is increasing evidence suggesting that patients with Borderline Personality Disorder (BPD) are at greater risk of developing cardiovascular diseases (CVD) compared to the general population. Homocysteine (Hcy) has been discussed as a serum marker for endothelial dysfunction as a mechanism involved in CVD and has been shown to be associated with numerous psychiatric conditions. Pathophysiologically, there seems to be a link between Hcy and psychological stress mediated by abnormal activity of the autonomic nervous system. Accordingly, the present study sought to examine Hcy in BPD and to explore possible associations with clinical parameters.

Methods

Plasma Hcy levels as well as conventional cardiovascular risk factors, such as blood pressure, BMI, smoking habits, HbA1c, HDL, LDL, and cholesterol, were examined in 49 young female in-patients diagnosed with BPD and 50 psychologically healthy control subjects matched for age and sex. Assessment of borderline symptom severity, childhood trauma, exposure to chronic stress, and quality of sleep was performed using self-reported questionnaires.

Results

BPD patients showed significantly higher mean plasma Hcy concentrations compared to controls, though below ranges considered pathological. Moreover, Hcy correlated significantly with the severity of childhood trauma, chronic stress, and subjective sleep disturbances. In a regression model BPD diagnosis was found to predict Hcy levels best.

Conclusion

In conclusion, young female BPD patients with no history of CVD show higher, though non-pathological, Hcy levels compared to healthy controls. Our findings seem to support the assumption that BPD is associated with increased risk of CVD, and that Hcy could serve as potential marker for risk evaluation of midlife CVD in BPD patients.
The non-linear nature of the cost of comprehensibility
Authors: Goethals Sofie , Martens David , Evgeniou Theodoros
Published: March 2022

A key challenge in Artificial Intelligence (AI) has been the potential trade-off between the accuracy and comprehensibility of machine learning models, as that also relates to their safe and trusted adoption. While there has been a lot of talk about this trade-off, there is no systematic study that assesses to what extent it exists, how often it occurs, and for what types of datasets. Based on the analysis of 90 benchmark classification datasets, we find that this trade-off exists for most (69%) of the datasets, but that somewhat surprisingly for the majority of cases it is rather small while for only a few it is very large. Comprehensibility can be enhanced by adding yet another algorithmic step, that of surrogate modelling using so-called ‘explainable’ models. Such models can improve the accuracy-comprehensibility trade-off, especially in cases where the black box was initially better. Finally, we find that dataset characteristics related to the complexity required to model the dataset, and the level of noise, can significantly explain this trade-off and thus the cost of comprehensibility. These insights lead to specific guidelines on how and when to apply AI algorithms when comprehensibility is required.
Machine learning-based turbulence-risk prediction method for the safe operation of aircrafts
Authors: Mizuno Shinya , Ohba Haruka , Ito Koji
Published: March 2022

This study has proposed a method for detecting turbulence, a primary factor that influences safe aircraft operation. The number of observed turbulence events is limited, thereby indicating the requirement of an appropriate flow for detecting turbulence events from a small number of samples. In addition, the opinions and experiences of pilots must be reflected at the initial stage to address the high risk of turbulence occurrence, which can result in airline operations being cancelled. Thus, this study proposed a method for predicting turbulence occurrence based on the turbulence occurrence date information provided by airlines as well as meteorological data sets obtained from open data available in Japan as teacher data. However, because commonly used machine learning methods are unable to detect the turbulence occurrence date, the proposed method employed principal component analysis coupled with the K-Means method to generate risk clusters with a high likelihood of turbulence occurrence and consequently perform statistical checks. Subsequently, the risk clusters were utilized as supervisory data for turbulence occurrence, while the support vector machine was used for predicting turbulence occurrence. Furthermore, the results obtained with the proposed method were statistically checked as well as practically verified by a pilot to confirm the appropriateness of the turbulence occurrence date predicted.
Single-step acid-catalyzed synthesis of luminescent colloidal organosilica nanobeads
Authors: Baipaywad Phornsawat , Hong Seong Vin , Kim Jong Bae , Hwang Jangsun , Choi Jonghoon , Park Hansoo , Paik Taejong
Published: March 2022

We present a single-step, room-temperature synthesis of fluorescent organosilica nanobeads (FOS NBs). The FOS NBs were synthesized under aqueous conditions using (3-aminopropyl)triethoxysilane (APTES) as the silicon source in the presence of l-ascorbic acid (L-AA). In the APTES/L-AA/water ternary phase, the hydrolysis and condensation reaction of APTES occurred under acidic conditions to form spherical FOS NBs with an average diameter of 426.8 nm. FOS NBs exhibit excellent colloidal stability in aqueous media. The formation of FOS NBs was complete within a 10 min reaction time, which indicates potential for large-scale mass-production synthesis of luminescent colloidal NBs. The FOS NBs exhibited blue photoluminescence (PL) under UV excitation in the absence of an additional high temperature calcination process or with the incorporation of any fluorophores. This phenomenon is attributed to the presence of carbon-containing defects, which act as luminescent centers formed by the reaction between amino groups in the APTES and l-ascorbic acid reductant. Finally, the results of a cytotoxicity test and cellular uptake experiments revealed that the FOS NBs showed potential as optical contrast agents for bioimaging.

Graphical Abstract
Surface and aloft NO2 pollution over the greater Tokyo area observed by ground-based and MAX-DOAS measurements bridged by kilometer-scale regional air quality modeling
Authors: Itahashi Syuichi , Irie Hitoshi
Published: March 2022

To advance our understanding of surface and aloft nitrogen dioxide (NO2) pollution, this study extensively evaluated NO2 concentrations simulated by the regional air quality modeling system with a 1.3 km horizontal grid resolution using the Atmospheric Environmental Regional Observation System ground-based observation network and aloft measurements by multi-axis differential optical absorption spectroscopy (MAX-DOAS) over the greater Tokyo area. Observations are usually limited to the surface level, and gaps remain in our understanding of the behavior of air pollutants above the near-surface layer, particularly within the planetary boundary layer (PBL). Therefore, MAX-DOAS measurement was used, which observes scattered sunlight in the ultraviolet/visible range at several elevation angles between the horizon and zenith to determine the aloft NO2 pollution averaged over 0–1 km. In total, four MAX-DOAS measurement systems at Chiba University (35.63°N, 140.10°E) systematically covered the north, east, west, and south directions to capture the aloft NO2 pollution over the greater Tokyo area. The target period was Chiba-Campaign 2015 conducted during 9–23 November 2015. The evaluations showed that the air quality modeling system can generally capture the observed behavior of both surface and aloft NO2 pollution in terms of spatial and temporal coverage. The diurnal variation, which typically showed an increase from evening to early morning without daylight and a decrease during the daytime, was also captured by the model. During Chiba-Campaign 2015, two cases of episodic higher NO2 concentration were identified: one during the nighttime and another during the daytime as different diurnal patterns. These were related to a stagnant wind field, with the latter also connected to a lower PBL height in cloudy conditions. Comparison of the modeled daily-averaged surface and aloft NO2 concentrations showed that aloft NO2 concentration exhibited a strong linear correlation with surface NO2 concentration, with the aloft (0–1 km) value scaled to 0.4–0.5-fold the surface value, irrespective of whether the day was clean or polluted. This scaling value was lower during the nighttime and higher during the daytime. Based on this synergetic analysis of surface and aloft observation bridged by a kilometer-scale fine-resolution modeling simulation, this study contributes to fostering understanding of aloft NO2 pollution.
Functional Impact of Post-exercise Cooling and Heating on Recovery and Training Adaptations: Application to Resistance, Endurance, and Sprint Exercise
Authors: Chaillou Thomas , Treigyte Viktorija , Mosely Sarah , Brazaitis Marius , Venckunas Tomas , Cheng Arthur J.
Published: March 2022

The application of post-exercise cooling (e.g., cold water immersion) and post-exercise heating has become a popular intervention which is assumed to increase functional recovery and may improve chronic training adaptations. However, the effectiveness of such post-exercise temperature manipulations remains uncertain. The aim of this comprehensive review was to analyze the effects of post-exercise cooling and post-exercise heating on neuromuscular function (maximal strength and power), fatigue resistance, exercise performance, and training adaptations. We focused on three exercise types (resistance, endurance and sprint exercises) and included studies investigating (1) the early recovery phase, (2) the late recovery phase, and (3) repeated application of the treatment. We identified that the primary benefit of cooling was in the early recovery phase (< 1 h post-exercise) in improving fatigue resistance in hot ambient conditions following endurance exercise and possibly enhancing the recovery of maximal strength following resistance exercise. The primary negative impact of cooling was with chronic exposure which impaired strength adaptations and decreased fatigue resistance following resistance training intervention (12 weeks and 4–12 weeks, respectively). In the early recovery phase, cooling could also impair sprint performance following sprint exercise and could possibly reduce neuromuscular function immediately after endurance exercise. Generally, no benefits of acute cooling were observed during the 24–72-h recovery period following resistance and endurance exercises, while it could have some benefits on the recovery of neuromuscular function during the 24–48-h recovery period following sprint exercise. Most studies indicated that chronic cooling does not affect endurance training adaptations following 4–6 week training intervention. We identified limited data employing heating as a recovery intervention, but some indications suggest promise in its application to endurance and sprint exercise.