bluuebunny/crossref_metadata_2025 huggingface dataset:
Perilla Fruit Oil-Fortified Soybean Milk Intake Alters Levels of Serum Triglycerides and Antioxidant Status, and Influences Phagocytotic Activity among Healthy Subjects: A Randomized Placebo-Controlled Trial
Authors: Koonyosying Pimpisid , Kusirisin Winthana , Kusirisin Prit , Kasempitakpong Boonsong , Sermpanich Nipon , Tinpovong Bow , Salee Nuttinee , Pattanapanyasat Kovit , Srichairatanakool Somdet , Paradee Narisara
Published: April 2022

This study aimed to develop perilla fruit oil (PFO)-fortified soybean milk (PFO-SM), identify its sensory acceptability, and evaluate its health outcomes. Our PFO-SM product was pasteurized, analyzed for its nutritional value, and had its acceptability assessed by an experienced and trained descriptive panel (n = 100) based on a relevant set of sensory attributes. A randomized clinical trial was conducted involving healthy subjects who were assigned to consume deionized water (DI), SM, PFO-SM, or black sesame-soybean milk (BS-SM) (n = 48 each, 180 mL/serving) daily for 30 d. Accordingly, health indices and analyzed blood biomarkers were recorded. Consequently, 1% PFO-SM (1.26 mg ALA rich) was generally associated with very high scores for overall acceptance, color, flavor, odor, taste, texture, and sweetness. We observed that PFO-SM lowered levels of serum triglycerides and erythrocyte reactive oxygen species, but increased phagocytosis and serum antioxidant activity (p < 0.05) when compared to SM and BS-SM. These findings indicate that PFO supplementation in soybean milk could enhance radical-scavenging and phagocytotic abilities in the blood of healthy persons. In this regard, it was determined to be more efficient than black sesame supplementation. We are now better positioned to recommend the consumption of PFO-SM drink for the reduction of many chronic diseases. Randomized clinical trial registration (Reference number 41389) by IRSCTN Registry.
Secretion and Detection of Defensive Compounds by the Red Flour Beetle Tribolium castaneum Interacting with the Insect Pathogenic Fungus Beauveria bassiana
Authors: Davyt-Colo Belén , Girotti Juan R. , González Andrés , Pedrini Nicolás
Published: April 2022

Entomopathogenic fungi such as Beauveria bassiana are extensively used for the control of insect pests worldwide. They infect mostly by adhesion to the insect surface and penetration through the cuticle. However, some insects, such as the red flour beetle Tribolium castaneum (Herbst), have evolved resistance by embedding their cuticle with antifungal compounds. Thus, they avoid fungal germination on the cuticle, which result in low susceptibility to entomopathogenic fungi. In adult T. castaneum, these antifungals are the well-known defensive compounds methyl-1,4- and ethyl-1,4-benzoquinone. In this study, we added B. bassiana conidia on the diet of adult beetles to study the effect of the entomopathogen on the secretion and detection of the beetle volatile blend containing both benzoquinones. The compounds were analyzed by solid phase microextraction coupled to gas chromatography–flame ionization detection, and were detected by electroantennography. In addition, we measured the expression level of four genes encoding for two odorant-binding proteins (OBP), one chemosensory protein (CSP), and one odorant receptor (OR) in both healthy and fungus-treated insects. Significant alterations in the secretion of both benzoquinones, as well as in the perception of methyl-1,4-benzoquinone, were found in fungus-treated insects. TcOBP7D, TcOBP0A and TcCSP3A genes were down-regulated in insects fed conidia for 12 and 48 h, and the latter gene was up-regulated in 72 h samples. TcOR1 expression was not altered at the feeding times studied. We conclude that fungus-treated insects alter both secretion and perception of benzoquinones, but additional functional and genetic studies are needed to fully understand the effects of fungal infection on the insect chemical ecology.
Assessment of Real-Time Quaking-Induced Conversion (RT-QuIC) Assay, Immunohistochemistry and ELISA for Detection of Chronic Wasting Disease under Field Conditions in White-Tailed Deer: A Bayesian Approach
Authors: Picasso-Risso Catalina , Schwabenlander Marc D. , Rowden Gage , Carstensen Michelle , Bartz Jason C. , Larsen Peter A. , Wolf Tiffany M.
Published: April 2022

Chronic wasting disease (CWD) is a transmissible prion disease of the cervidae family. ELISA and IHC tests performed postmortem on the medial retropharyngeal lymph nodes (RPLN) or obex are considered diagnostic gold standards for prion detection. However, differences in CWD transmission, stage of infection, pathogenesis, and strain can limit performance. To overcome these uncertainties, we used Bayesian statistics to assess the accuracy of RT-QuIC, an increasingly used prion amplification assay, to diagnose CWD on tonsil (TLN), parotid (PLN) and submandibular lymph nodes (SMLN), and ELISA/IHC on RPLN of white-tailed deer (WTD) sampled from Minnesota. Dichotomous RT-QuIC and ELISA/IHC results from wild (n = 61) and captive (n = 46) WTD were analyzed with two-dependent-test, one-population models. RT-QuIC performed on TLN and SMLN of the wild WTD population had similar sensitivity (median range (MR): 92.2–95.1) to ELISA/IHC on RPLN (MR: 91.1–92.3). Slightly lower (4–7%) sensitivity estimates were obtained from farmed animal and PLN models. RT-QuIC specificity estimates were high (MR: 94.5–98.5%) and similar to ELISA/IHC estimates (MR: 95.7–97.6%) in all models. This study offers new insights on RT-QuIC and ELISA/IHC performance at the population level and under field conditions, an important step in CWD diagnosis and management.
Ceragenin CSA-44 as a Means to Control the Formation of the Biofilm on the Surface of Tooth and Composite Fillings
Authors: Tokajuk Joanna , Deptuła Piotr , Chmielewska Sylwia J , Skłodowski Karol , Mierzejewska Żaneta A , Grądzka-Dahlke Małgorzata , Tołstoj Adam , Daniluk Tamara , Paprocka Paulina , Savage Paul B , Bucki Robert
Published: April 2022

Recurrent oral infections, as manifested by endodontic and periodontal disease, are often caused by Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans). Here, we assessed the anti-biofilm activity of ceragenin CSA-44 against these microbes growing as a biofilm in the presence of saliva on the surface of human teeth and dental composite (composite filling) subjected to mechanical stresses. Methods: Biofilm mass analysis was performed using crystal violet (CV) staining. The morphology, viscoelastic properties of the biofilm after CSA-44 treatment, and changes in the surface of the composite in response to biofilm presence were determined by AFM microscopy. Results: CSA-44 prevented biofilm formation and reduced the mass of biofilm formed by tested microorganisms on teeth and dental composite. Conclusion: The ability of CSA-44 to prevent the formation and to reduce the presence of established biofilm on tooth and composite filling suggests that it can serve as an agent in the development of new methods of combating oral pathogens and reduce the severity of oral infections.
Drug Candidates for Autoimmune Diseases
Authors: Saurin Sabrina , Meineck Myriam , Erkel Gerhard , Opatz Till , Weinmann-Menke Julia , Pautz Andrea
Published: April 2022

Most of the immunosuppressive drugs used in the clinic to prevent organ rejection or to treat autoimmune disorders were originally isolated from fungi or bacteria. Therefore, in addition to plants, these are valuable sources for identification of new potent drugs. Many side effects of established drugs limit their usage and make the identification of new immunosuppressants necessary. In this review, we present a comprehensive overview of natural products with potent anti-inflammatory activities that have been tested successfully in different models of chronic inflammatory autoimmune diseases. Some of these candidates already have passed first clinical trials. The anti-inflammatory potency of these natural products was often comparable to those of established drugs, and they could be used at least in addition to standard therapy to reduce their dose to minimize unwanted side effects. A frequent mode of action is the inhibition of classical inflammatory signaling pathways, such as NF-κB, in combination with downregulation of oxidative stress. A drawback for the therapeutic use of those natural products is their moderate bioavailability, which can be optimized by chemical modifications and, in addition, further safety studies are necessary. Altogether, very interesting candidate compounds exist which have the potential to serve as starting points for the development of new immunosuppressive drugs.
A Multi-Layered Borophene-Silica-Silver Based Refractive Index Sensor for Biosensing Applications Operated at the Infrared Frequency Spectrum
Authors: Alharbi Abdullah G. , Sorathiya Vishal , Lavadiya Sunil
Published: April 2022

We have presented the borophene based refractive index sensor for the infrared frequency spectrum of 188 to 250 THz (1.2–1.6 µm) range. The proposed structure was formed by using the Silver-borophene-silica-Ag layered structure. The behaviour of the different analyte (with a different refractive index) material is numerically calculated by placing it on the top of the structure. The behaviour of the structure is identified in terms of absorption, reflectance, physical parameter variation, and oblique angle incident conditions. The presented results provide the basic idea of selecting optimized structure dimensions to get the specific resonating response. This sensor offers the Figure of Merit (FOM) of 444 RIU−1 with high sensitivity of 660 THz/RIU (4471 nm/RIU). The refractive index sensor also provides wide-angle stability for (0° to 80°) for the wide frequency range (239 to 245 THz and 207 to 209 THz). This sensor is developed on the silver metal layer (not required to separate borophene from its origin metal deposition process) and easily fabricated using standard boron fabrication and layered deposition techniques. The results of the proposed structure make it possible to design a basic biosensor structure. This device is also applicable for various THz and biomedical applications.
Comparison between Quality Traits of Solar-Dried and Freeze-Dried Origanum syriacum L. (Za’atar)
Authors: Alwafa Reem Abu , Mudalal Samer , Shraim Faisal , Mauriello Gianluigi
Published: April 2022

There is growing interest in Origanum syriacum due to attractive culinary applications and functional properties. Solar drying is the most common conventional method for drying Origanum syriacum L. (Za’atar) in Mediterranean region. This study aimed to evaluate the quality traits of solar dried za’atar in comparison to freeze dried za’atar. Proximate composition (moisture, protein, fat, fiber, carbohydrates, and ash), color index (L*a*b*), macro and microstructure, sensory, and microbiological characteristics were evaluated. Solar dried za’atar exhibited significantly lower fat content (1.10 vs. 1.64%, p < 0.05) than freeze dried za’atar. Solar drying led to severe changes in color values compared to freeze drying. Solar drying exhibited loss in the structural integrity and leave hairs more than freeze drying. Moreover, freeze-dried za’atar exhibited significantly lower total aerobic count (4.92 vs. 5.23, p < 0.05) and yeasts and mold count (4.59 vs. 5.36, p < 0.05) than solar-dried za’atar. Panelists were able significantly to differentiate between solar- and freeze dried za’atar. Freeze-dried za’atar had significantly lower hedonic score of color, odor, taste, and overall acceptance than solar dried za’atar. In conclusion, using freeze drying achieved good improvements in some quality traits for za’atar while solar dried za’atar showed better sensory perception.
Grape Cultivar Features Differentiate the Grape Rhizosphere Microbiota
Authors: Bao Lijun , Sun Bo , Wei Yingxue , Xu Nan , Zhang Shiwei , Gu Likun , Bai Zhihui
Published: April 2022

Rhizosphere microflora are key determinants that contribute to plant growth and productivity, which are involved in improving the uptake of nutrients, regulation of plants’ metabolisms and activation of plants’ responses against both biotic and abiotic stresses. However, the structure and diversity of the grape rhizosphere microbiota remains poorly described. To gain a detailed understanding of the assembly of rhizosphere microbiota, we investigated the rhizosphere microbiota of nine grape varieties in northern China by high-throughput sequencing. We found that the richness and diversity of bacterial and fungal community networking in the root compartments were significantly influenced by the grape variety. The bacterial linear discriminant analysis showed that Pseudomonas and Rhizobium, which were considered as potential plant-growth-promoting bacteria, were more enriched in Pinot noir, and Nitrosospira was enriched in Gem. The fungal linear discriminant analysis showed that Fusarium was more enriched in Longan, Sporormiella was more enriched in Merlot, Gibberella and Pseudallescheria were more enriched in Gem and Mortierella was more abundant in Cabernet Sauvignon. The 16S rRNA functional prediction indicated that no significance differentiates among the grape varieties. Understanding the rhizosphere soil microbial diversity characteristics of different grape varieties could provide the basis for exploring microbial associations and maintaining the health of grapes.
Correlation of Glucosinolates and Volatile Constituents of Six Brassicaceae Seeds with Their Antioxidant Activities Based on Partial Least Squares Regression
Authors: Khalil Noha , Gad Haidy A. , Al Musayeib Nawal M. , Bishr Mokhtar , Ashour Mohamed L.
Published: April 2022

Brassicaceae comprises various species representing an economically important source of industrial or pharmaceutical crops. The present study aimed to identify glucosinolates (GSLs) and volatile compounds in six Brassicaceae seeds cultivated in Egypt. An (High Performance Liquid Chromatography-Photodiode Array) HPLC–PDA analysis of GSLs in the alcoholic extracts of Raphanus raphanistrum L. (Rr), Raphanus sativus L. (Rs), Brassica oleracea var. capitata L. (Boc), Brassica oleracea var. botrytis L. (Bob), Brassica rapa L. (Br), and Eruca sativa L. (Es) was carried out using a mixture of 23 standard GSLs. Nineteen GSLs were detected in the studied seeds. Rs had the highest GSL content (135.66 μmol/g Dry weight, DW), while Boc had the lowest GSL content (93.66 μmol/g DW). Glucobrassicin was the major identified compound in Rr, Rs, and Bob. Its highest content was in Rs (28.96 μmol/g DW). Sinigrin was the major identified GSL in Boc (18.02 μmol/g DW), although present with higher content in Bob (22.02 μmol/g DW). Neoglucobrassicin was the major GSL in Br (30.98 μmol/g DW), while glucoerucin was the major GSL in Es (17.84 μmol/g DW). The yields of the steam-distilled oils of the studied seeds ranged between 3.25 ± 0.36 and 9.68 ± 0.25% v/w. A GC–MS analysis of the oils could detect 3, 23, 18, 16, 7, and 9 compounds in Rr, Rs, Boc, Bob, Br, and Es oils, respectively. Sulfur and nitrogenous compounds predominated in all studied oils except Rs, which contained a higher percentage of alkanes. The major identified compound in Rr oil was 4-isothiocyanato-1-(methylthio)-1-butene (94.77 ± 1.25%), while in Br it was 3-butenyl isothiocyanate (69.55 ± 1.02%), thiolane in Rs (15.15 ± 0.22%), and erucin in Es (97.02 ± 1.514%). Both Boc and Bob had the same major compound 4-(methylthio) butanenitrile, which represented 40.35 ± 1.15 and 50.52 ± 1.02% in both oils, respectively. Radical scavenging activity for both GSL extracts and essential oils on DPPH radical ranged between 18.01 ± 0.72 and 114.28 ± 1.15 µg/mL (IC50). The highest antioxidant capacity was for Es oil, while the lowest one was for Rr oil. Generally, it was observed that the GSLs had better antioxidant activity than their corresponding essential oils except for Es oil, which had higher activity. A principal component analysis (PCA) was successfully applied to discriminate among six Brassicaceae seeds based on both HPLC and GC–MS, where complete segregation was achieved among all samples with high correlation between Boc and Bob. Partial Least Squares-Regression (PLS-R) models showed that there is a better correlation between the antioxidant activity and glucosinolate profile when being compared to that of a volatile one. This profiling and variation of GSLs and volatile metabolites of the studied Brassicaceae seeds may be employed in further studies regarding their health-promoting properties.
Phylogenetic Marker Selection and Protein Sequence Analysis of the ORF5 Gene Product of Grapevine Virus A
Authors: Rastgou Mina , Roumi Vahid , Noris Emanuela , Matić Slavica , Ercisli Sezai
Published: April 2022

Grapevine virus A (GVA), the type species of the Vitivirus genus, is one of the causal agents of the Kober stem grooving disease of the rugose wood complex and one of the most frequently detected viruses in grapevine. There is little information on GVA gene(s) marker useful for phylogenetic analysis. To this aim, a total of 403 leaf samples were collected from vineyards of East and West Azarbaijan provinces in the Northwestern provinces of Iran during 2014–2016 and tested by DAS-ELISA and RT-PCR using ORF5-specific primers. GVA was detected in 56 symptomatic samples, corresponding to 14% of infection, while it was not detected in asymptomatic samples. The ORF5 (p10) protein sequence of eight Iranian isolates was compared to other vitiviruses, showing that the most conserved region resides in the N-terminus, carrying an arginine-rich motif followed by a zinc-finger motif. Next, to define a robust phylogenetic marker representative of the whole genome sequence suitable for phylogenetic and evolutionary studies, phylogenetic trees based on the full genome sequences of all the available GVA isolates and on individual genomic regions were constructed and compared. ORF1, which encodes the RNA-dependent RNA polymerase, was found to be the best phylogenetic marker for GVA classification and evolution studies. These results can be used for further research on phylogenetic analyses, evolution history, epidemiology, and etiology of rugose wood complex, and to identify control measures against GVA and other vitiviruses.
Breeding Diploid F1 Hybrid Potatoes for Propagation from Botanical Seed (TPS): Comparisons with Theory and Other Crops
Authors: Bradshaw John E.
Published: April 2022

This paper reviews the progress and the way ahead in diploid F1 hybrid potato breeding by comparisons with expectations from the theory of inbreeding and crossbreeding, and experiences from other diploid outbreeding crops. Diploid potatoes can be converted from an outbreeding species, in which self-pollination is prevented by a gametophytic self-incompatibility system, into one where self-pollination is possible, either through a dominant self-incompatibility inhibitor gene (Sli) or knockout mutations in the incompatibility locus. As a result, diploid F1 hybrid breeding can be used to produce genetically uniform potato cultivars for propagation from true potato seeds by crossing two near-homozygous inbred lines, derived from a number of generations of self-pollination despite inbreeding depression. Molecular markers can be used to detect and remove deleterious recessive mutations of large effect, including those in tight repulsion linkage. Improvements to the inbred lines can be made by introducing and stacking genes and chromosome segments of large desirable effect from wild relatives by backcrossing. Improvements in quantitative traits require a number of cycles of inbreeding and crossbreeding. Seed production can be achieved by hand pollinations. F1 hybrid planting material can be delivered to farmers as true seeds or young plants, and mini-tubers derived from true seeds.
Investigation into Layer Number Effect on Breakdown Strength of Multi-Layer Polymer Films
Authors: Zhao Liang , Yu Binxiong , Shang Wei
Published: April 2022

The layer number effect on electric breakdown strength (EBD) of multi-layer polymer films is investigated using 10-μm polypropylene (PP) films under a dc condition. The layer number, n, of the films during the test is as large as 120. It is observed that the relation between EBD and n conforms to a minus power law, i.e., EBD(n) = E1′n−a, where the power exponent, a, is 0.27, E1′ is a constant. By reviewing the experimental data in references, it is found that the power law holds true for different types of polymers in different test conditions, but the value of a varies from 0.072 to 0.5. The variation of a is explained in perspective of the discontinuous structures within films and those between films. A small value of a means a good purity level of the film, which is due to the decrease of the size of the inter-layer defects. A large value of a means a poor purity level of the films, which is due to the increase of the amount of intra-layer defects. Both factors influence the value of a, leading to the variation of a.
Analysis of Correlation between Structure and Properties of Carboxymethyl Cellulose Film Loaded with Eu3+ and Tb3+ Fluorescence by Rheology at Different Drying Stages
Authors: Ye Jun , Fu Zichang , Rao Jiawei , Xiong Jian
Published: April 2022

The influences of interactions between carboxymethyl cellulose (CMC) and CMC/europium (III)–terbium (III) (CET) on the structure and properties of the resultant CMC/CET films were investigated by rheology at three stages of the film-drying process. According to the water content at different drying times, the kinetics curves during the film-drying process were drawn. Then, the rheology properties of film-forming solutions during the drying process were characterized by dynamic modulus, Han plots, zero shear complex viscosity and relaxation time. When the water content was 90%, the film contained either 0.1 or 0.2 g of CET, which had good fluidity, while the film with 0.3 g of CET was elastic-dominated. Han plots and XRD analyses showed that the interactions between the CMC and CET were not hydrogen bonds but random entanglements. The zero-shear complex viscosity and relaxation time spectrum confirmed that the entanglements enhanced as the CET content increased. Meanwhile, aggregation formed in the solution of CMC with 0.3 g of CET. When CMC-CET films with different CET additions were compared, the film with 0.2 g of CET had an even and tight sheet structure, the greatest fluorescence intensity, and superior tensile strength of 78.76 MPa.
Aqueous Strippable Polymer Coating for Highly Efficient Primary Radioactive Uranium Decontamination with Versatility on Diversified Surface
Authors: Xue Yang , Yang Wuxinchen , Yue Renliang , Chen Yunfa
Published: April 2022

The decontamination of radioactive materials on the surfaces of nuclear facilities has generated large quantities of waste from the rapid development of the nuclear industry, posing a potential threat globally. Strippable coating has been employed for some time to remove radioactive contamination due to its high performance and removability, flexibility, and compatibility with various substrates. Herein, an aqueous strippable coating based on an adsorbent/polyvinyl alcohol (PVA) polymer was developed to remove radioactive uranium from stainless-steel surfaces that showed greater decontamination than that of DeconGel, with an efficiency of 87.2% for 5 g/L uranium and 95.5% for 22.5 g/L uranium, along with a high repeatability and better mechanical properties. Furthermore, the prepared coating was versatile and could be applied to a range of substrate surfaces (lacquered, aluminum, glass, plastic, and ceramic), with outstanding performance ranging from 79.2 to 95.4% for 1 g/L uranium. The prepared coating could also be applied through brushing or spraying to horizontal or vertical substrates. The exceptional performance could be due to the synergistic effect of the introduction of ethylene diamine tetra-acetic acid disodium salt (EDTA-2Na) as a chelating agent and the nano-adsorbent CaCO3/TiO2.
pH- and Thermo-Responsive Water-Soluble Smart Polyion Complex (PIC) Vesicle with Polyampholyte Shells
Authors: Pham Thu Thao , Pham Tien Duc , Yusa Shin-ichi
Published: April 2022

A diblock copolymer (P(VBTAC/NaSS)17-b-PAPTAC50; P(VS)17A50) composed of amphoteric random copolymer, poly(vinylbenzyl trimethylammonium chloride-co-sodium p-styrensunfonate) (P(VBTAC/NaSS); P(VS)) and cationic poly(3-(acrylamidopropyl) trimethylammonium chloride) (PAPTAC; A) block, and poly(acrylic acid) (PAAc49) were prepared via a reversible addition−fragmentation chain transfer radical polymerization. Scrips V, S, and A represent VBTAC, NaSS, and PAPTAC blocks, respectively. Water-soluble polyion complex (PIC) vesicles were formed by mixing P(VS)17A50 and PAAc49 in water under basic conditions through electrostatic interactions between the cationic PAPTAC block and PAAc49 with the deprotonated pendant carboxylate anions. The PIC vesicle collapsed under an acidic medium because the pendant carboxylate anions in PAAc49 were protonated to delete the anionic charges. The PIC vesicle comprises an ionic PAPTAC/PAAc membrane coated with amphoteric random copolymer P(VS)17 shells. The PIC vesicle showed upper critical solution temperature (UCST) behavior in aqueous solutions because of the P(VS)17 shells. The pH- and thermo-responsive behavior of the PIC vesicle were studied using 1H NMR, static and dynamic light scattering, and percent transmittance measurements. When the ratio of the oppositely charged polymers in PAPTAC/PAAc was equal, the size and light scattering intensity of the PIC vesicle reached maximum values. The hydrophilic guest molecules can be encapsulated into the PIC vesicle at the base medium and released under acidic conditions. It is expected that the PIC vesicles will be applied as a smart drug delivery system.
Preparation and Analysis of Sheath–Core Intelligent Thermo-Regulating Fiber
Authors: Zhang Ronggen , Feng Pei , Yang Chongchang
Published: April 2022

In this work, a skin–core composite intelligent temperature-adjusting fiber was prepared using the composite melt spinning method, with polypropylene as the skin layer and T28-type paraffin as the core layer, in order to obtain clothing fibers with a bidirectional temperature adjustment function. A differential scanning calorimeter was used to test the phase-change latent heat of fibers with different amounts of paraffin injections, and an infrared thermal imager was used to monitor the skin–core composite intelligent temperature-adjusting fiber bundles and ordinary polypropylene fiber bundles under the same heating and cooling conditions. The temperature of the fiber bundle was considered to be a function of time. The results showed that with the increase in the amount of the paraffin injections, the proportion of the paraffin component in the fiber and the latent heat of the fiber phase transition also increased. When the paraffin injection amount was 1.5 mL/min, the melting enthalpy and the crystallization enthalpy reached 65.93 J/g and 66.15 J/g, respectively. Under the same conditions, the heating speed of the intelligent temperature-adjusting fiber bundle was found to be slower than that of the ordinary polypropylene fibers, and the maximum temperature difference between the two reached 8.0 °C. Further, the cooling speed of the former was also observed to be slower than that of the latter, and the maximum temperature difference between the two reached 6.7 °C.
Mechanical Behavior and Energy Dissipation of Woven and Warp-Knitted Pvc Membrane Materials under Multistage Cyclic Loading
Authors: Guo Shanshan , Wang Linlin , Shao Guangwei , Shao Huiqi , Jiang Jinhua , Chen Nanliang
Published: April 2022

In order to study the mechanical behavior and energy dissipation of architectural membrane materials under multistage cyclic loading, the deformation behavior, energy dissipation, and damage characteristics of four kinds of warp-knitted and woven polyvinyl chloride (PVC) membrane materials were analyzed using multistage cyclic loading experiments. The results show that, compared with the uniaxial tensile strength, the peak values of the cyclic loading and unloading of the four material samples are lower in the warp direction but higher in the fill (weft) direction. Under multistage cyclic loading, the loading and unloading moduli of the warp knitting membrane increase with the increase in fabric density. At the same fabric density, the loading modulus and the unloading modulus are smaller than those of the warp knitting material. The total absorbed strain energy, elastic strain energy, and dissipation energy of the fill samples are higher than those of the warp samples at a low load level but lower than those at a high load level. PVC membrane materials’ use strength should be controlled below a 15% stress level under long-term external force loading. In the cyclic loading process, the four PVC membrane materials are viscoelastic–plastic, so it is reasonable to define the damage variable based on the accumulation of plastic deformation.
Applications of Polymers for Organ-on-Chip Technology in Urology
Authors: Galateanu Bianca , Hudita Ariana , Biru Elena Iuliana , Iovu Horia , Zaharia Catalin , Simsensohn Eliza , Costache Marieta , Petca Razvan-Cosmin , Jinga Viorel
Published: April 2022

Organ-on-chips (OOCs) are microfluidic devices used for creating physiological organ biomimetic systems. OOC technology brings numerous advantages in the current landscape of preclinical models, capable of recapitulating the multicellular assemblage, tissue–tissue interaction, and replicating numerous human pathologies. Moreover, in cancer research, OOCs emulate the 3D hierarchical complexity of in vivo tumors and mimic the tumor microenvironment, being a practical cost-efficient solution for tumor-growth investigation and anticancer drug screening. OOCs are compact and easy-to-use microphysiological functional units that recapitulate the native function and the mechanical strain that the cells experience in the human bodies, allowing the development of a wide range of applications such as disease modeling or even the development of diagnostic devices. In this context, the current work aims to review the scientific literature in the field of microfluidic devices designed for urology applications in terms of OOC fabrication (principles of manufacture and materials used), development of kidney-on-chip models for drug-toxicity screening and kidney tumors modeling, bladder-on-chip models for urinary tract infections and bladder cancer modeling and prostate-on-chip models for prostate cancer modeling.
Dependable Performance of Thin Film Composite Nanofiltration Membrane Tailored by Capsaicin-Derived Self-Polymer
Authors: Tang Yuanyuan , Cao Lu , Xu Li , Wang Zhaoyu , Shi Qian , Zhang Yingying , Yu Liangmin
Published: April 2022

To address trade-off and membrane-fouling challenges during the development of nanofiltration membranes, a thin-film composite membrane was prepared on the basis of interfacial polymerization regulated by adjusting the capsaicin-derived self-polymer poly N-(2-hydroxy-5-(methylthio) benzyl) acrylamide (PHMTBA) on the polysulfone substrate in this study. Through the self-polymerization of the monomer HMTBA with varied contents, microwave-assisted technology was employed to develop a variety of PHMTBAs. It was discovered that PHMTBA is involved in the interfacial polymerization process. Piperazine and PHMTBA competed for the reaction with trimesoyl chloride, resulting in a flatter and looser membrane surface. The PHMTBA-modified membrane presented a typical double-layer structure: a thicker support layer and a thinner active layer. The addition of PHMTBA to membranes improved their hydrophilicity and negative charge density. As a result, the PHMTBA-modified membrane showed dependable separation performance (water flux of 159.5 L m−2 h−1 and rejection of 99.02% for Na2SO4) as well as enhanced anti-fouling properties (flux recovery ratio of more than 100% with bovine serum albumin-fouling and antibacterial efficiency of 93.7% against Escherichia coli). The performance of the prepared membranes was superior to that of most other modified TFC NF membranes previously reported in the literature. This work presents the application potential of capsaicin derivatives in water treatment and desalination processes.
Silicalite-1/PDMS Hybrid Membranes on Porous PVDF Supports: Preparation, Structure and Pervaporation Separation of Dichlorobenzene Isomers
Authors: He Qiuping , Chen Wei , Wang Pengfei , Dou Xiaoming
Published: April 2022

Separation of dichlorobenzene (DCB) isomers with high purity by time− and energy−saving methods from their mixtures is still a great challenge in the fine chemical industry. Herein, silicalite-1 zeolites/polydimethylsiloxane (PDMS) hybrid membranes (silicalite-1/PDMS) have been successfully fabricated on the porous polyvinylidene fluoride (PVDF) supports to first investigate the pervaporation separation properties of DCB isomers. The morphology and structure of the silicalite-1 zeolites and the silicalite-1/PDMS/PVDF hybrid membranes were characterized by XRD, FTIR, SEM and BET. The results showed that the active silicalite-1/PDMS layers were dense and continuous without any longitudinal cracks and other defects with the silicalite-1 zeolites content no more than 10%. When the silicalite-1 zeolites content exceeded 10%, the surfaces of the active silicalite-1/PDMS layers became rougher, and silicalite-1 zeolites aggregated to form pile pores. The pervaporation experiments both in single-isomer and binary−isomer systems for the separation of DCB isomers was further carried out at 60 °C. The results showed that the silicalite-1/PDMS/PVDF hybrid membranes with 10% silicalite-1 zeolites content had better DCB selective separation performance than the silicalite-1/α−Al2O3 membranes prepared by template method. The permeate fluxes of the DCB isomers increased in the order of m−DCB < o−DCB < p−DCB both in single-isomer and binary-isomers solutions for the silicalite-1/PDMS/PVDF hybrid membranes. The separation factor of the silicalite-1/PDMS/PVDF hybrid membranes for p/o−DCB was 2.9 and for p/m−DCB was 4.6 in binary system. The permeate fluxes of the silicalite-1/PDMS/PVDF hybrid membranes for p−DCB in p/o−DCB and p/m−DCB binary−isomers solutions were 126.2 g∙m−2∙h−1 and 104.3 g∙m−2∙h−1, respectively. The thickness−normalized pervaporation separation index in p/o−DCB binary−isomers solutions was 4.20 μm∙kg∙m−2∙h−1 and in p/m−DCB binary−isomers solutions was 6.57 μm∙kg∙m−2∙h−1. The results demonstrated that the silicalite-1/PDMS/PVDF hybrid membranes had great potential for pervaporation separation of DCB from their mixtures.
A Synthetic Chart for Internal Stability Assessment of Soils Based on Soil PSD Curves
Authors: Lai Yongbiao , Bai Songsong , Hou Jian , Zhou Zongqing , Wu Qiangling , Lv Xiaobo , Yang Liming , Cao Weixun , Ren Zhengtao
Published: April 2022

Awareness of granulated soils’ internal instability is an important parameter when designing granulated filters, and the ability of a granulated soil’s internal stability can be verified using the soil PSD (Particle Size Distribution) curves’ secant slopes. The current work presents a new method to calculate the soil PSD curves’ secant slopes automatically, and a synthetic diagram is presented for the potential examination of the granulated soils’ internal stability. To verify the feasibility and accuracy of this synthetic diagram, 80 specimens of soil were investigated in this work and categorized into two groups: 50 sand–gravel soils and 30 (clay)–silt–sand–gravel soils. The obtained conclusions indicate that the internal stability and instability potentials of sand–gravel soils can be distinguished successfully with a synthetic chart and the Kenney and Lau criterion but cannot be assessed with the Kezdi and Sherard criteria. None of the criteria studied here can be used for the internal stability assessment of silt–sand–gravel and clay–silt–sand–gravel soils.
Digital Twins for scFv Production in Escherichia coli
Authors: Helgers Heribert , Hengelbrock Alina , Schmidt Axel , Vetter Florian Lukas , Juckers Alex , Strube Jochen
Published: April 2022

Quality-by-Design (QbD) is demanded by regulatory authorities in biopharmaceutical production. Within the QbD frame advanced process control (APC), facilitated through process analytical technology (PAT) and digital twins (DT), plays an increasingly important role as it can help to assure to stay within the predefined proven acceptable range (PAR).This ensures high product quality, minimizes failure and is an important step towards a real-time-release testing (RTRT) that could help to accelerate time-to-market of drug substances, which is becoming even more important in light of dynamical pandemic situations. The approach is exemplified on scFv manufacturing in Escherichia coli. Simulation results from digital twins are compared to experimental data and found to be accurate and precise. Harvest is achieved by tangential flow filtration followed by product release through high pressure homogenization and subsequent clarification by tangential flow filtration. Digital twins of the membrane processes show that shear rate and transmembrane pressure are significant process parameters, which is in line with experimental data. Optimized settings were applied to 0.3 bar and a shear rate of 11,000 s−1. Productivity of chromatography steps were 5.3 g/L/d (Protein L) and 2167 g/L/d (CEX) and the final product concentration was 8 g/L. Based on digital twin results, an optimized process schedule was developed that decreased purification time to one working day, which is a factor-two reduction compared to the conventional process schedule. This work presents the basis for future studies on advanced process control and automation for biologics production in microbials in regulated industries.
From Dialogue to Revelation: Alterity and the Concept of Fraternity (Fraternité) in Léon Askenazi’s Biblical Hermeneutics
Authors: Werdiger Ori
Published: April 2022

In Love: Accusative and Dative, Paul Mendes-Flohr explores ancient and modern Jewish engagements with the commandment to love the Re’a (neighbor) in Leviticus 19:18. Drawing on Rosenzweig’s phenomenology of divine–human love, Mendes-Flohr seeks to delineate the possibility of a humanist ethics of compassion that is not dependent, as in Rosenzweig, on hearing the divine voice. Taking Mendes-Flohr as point of departure, this paper explores the concept of fraternity (fraternité) as it figures in the thought of Yehuda Léon Askenazi (1922–1996), a North African kabbalist thinker and an important spiritual leader of Francophone Jewry in the twentieth century. Looking at two interrelated moments in Askenazi’s long career as a biblical exegete, I quarry Askenazi’s notion of fraternity for an account of alterity. Based on his discussions of the Cain and Abel story, as well as other biblical episodes, I argue that, for Askenazi, the challenge of fraternity, as figuring repeatedly in the Genesis narrative, is the preferred model to think of second-person relationships. Furthermore, I suggest, in contrast to Rosenzweig’s top-down account of revelation and human love, Askenazi’s approach represents a bottom-up model of love of one’s neighbor, which, when achieved, brings about divine revelation.
Plane-Based Robust Registration of a Building Scan with Its BIM
Authors: Sheik Noaman Akbar , Deruyter Greet , Veelaert Peter
Published: April 2022

The registration of as-built and as-planned building models is a pre-requisite in automated construction progress monitoring. Due to the numerous challenges associated with the registration process, it is still performed manually. This research study proposes an automated registration method that aligns the as-built point cloud of a building to its as-planned model using its planar features. The proposed method extracts and processes all the plane segments from both the as-built and the as-planned models, then—for both models—groups parallel plane segments into clusters and subsequently determines the directions of these clusters to eventually determine a range of possible rotation matrices. These rotation matrices are then evaluated through a computational framework based on a postulation concerning the matching of plane segments from both models. This framework measures the correspondence between the plane segments through a matching cost algorithm, thus identifying matching plane segments, which ultimately leads to the determination of the transformation parameters to correctly register the as-built point cloud to its as-planned model. The proposed method was validated by applying it to a range of different datasets. The results proved the robustness of the method both in terms of accuracy and efficiency. In addition, the method also proved its correct support for the registration of buildings under construction, which are inherently incomplete, bringing research a step closer to practical and effective construction progress monitoring.
Stand Canopy Closure Estimation in Planted Forests Using a Geometric-Optical Model Based on Remote Sensing
Authors: Yang Xiguang , He Ping , Yu Ying , Fan Wenyi
Published: April 2022

Canopy closure, which is the ratio of the vertical projection area of the crowns to the area of forest land, can indicate the growth and tending situation of a forest and is of great significance for forest management planning. In this study, a geometric-optical model (GOST model) was used to simulate the canopy gap fraction of a forest. Then, a canopy closure estimation method using the gap fraction was discussed. In this study, three typical planted forest farms (the Mengjiagang (MJG), Gaofeng (GF), and Wangyedian (WYD) forest farms) containing the most commonly planted tree species in the north and south regions of China were selected, and field measurements were executed. The results show that the gap fraction (Pvg-c) had a higher correlation with the average projected area of the tree crowns, and the relationship was an exponential function, with R2 and RMSE values of 0.5619 and 0.0723, respectively. Finally, the applicability and accuracy of this method were evaluated using line transects, and a fisheye camera measured the canopy closure. The accuracy of the canopy closure estimated by the Pvg-c was 86.69%. This research can provide a reference for canopy closure estimation using a geometric-optical model.
Adversarial Resolution Enhancement for Electrical Capacitance Tomography Image Reconstruction
Authors: Deabes Wael , Abdel-Hakim Alaa E. , Bouazza Kheir Eddine , Althobaiti Hassan
Published: April 2022

High-quality image reconstruction is essential for many electrical capacitance tomography (CT) applications. Raw capacitance measurements are used in the literature to generate low-resolution images. However, such low-resolution images are not sufficient for proper functionality of most systems. In this paper, we propose a novel adversarial resolution enhancement (ARE-ECT) model to reconstruct high-resolution images of inner distributions based on low-quality initial images, which are generated from the capacitance measurements. The proposed model uses a UNet as the generator of a conditional generative adversarial network (CGAN). The generator’s input is set to the low-resolution image rather than the typical random input signal. Additionally, the CGAN is conditioned by the input low-resolution image itself. For evaluation purposes, a massive ECT dataset of 320 K synthetic image–measurement pairs was created. This dataset is used for training, validating, and testing the proposed model. New flow patterns, which are not exposed to the model during the training phase, are used to evaluate the feasibility and generalization ability of the ARE-ECT model. The superiority of ARE-ECT, in the efficient generation of more accurate ECT images than traditional and other deep learning-based image reconstruction algorithms, is proved by the evaluation results. The ARE-ECT model achieved an average image correlation coefficient of more than 98.8% and an average relative image error about 0.1%.
Segmentation Performance Comparison Considering Regional Characteristics in Chest X-ray Using Deep Learning
Authors: Lee Hyo Min , Kim Young Jae , Kim Kwang Gi
Published: April 2022

Chest radiography is one of the most widely used diagnostic methods in hospitals, but it is difficult to read clearly because several human organ tissues and bones overlap. Therefore, various image processing and rib segmentation methods have been proposed to focus on the desired target. However, it is challenging to segment ribs elaborately using deep learning because they cannot reflect the characteristics of each region. Identifying which region has specific characteristics vulnerable to deep learning is an essential indicator of developing segmentation methods in medical imaging. Therefore, it is necessary to compare the deep learning performance differences based on regional characteristics. This study compares the differences in deep learning performance based on the rib region to verify whether deep learning reflects the characteristics of each part and to demonstrate why this regional performance difference has occurred. We utilized 195 normal chest X-ray datasets with data augmentation for learning and 5-fold cross-validation. To compare segmentation performance, the rib image was divided vertically and horizontally based on the spine, clavicle, heart, and lower organs, which are characteristic indicators of the baseline chest X-ray. Resultingly, we found that the deep learning model showed a 6–7% difference in the segmentation performance depending on the regional characteristics of the rib. We verified that the performance differences in each region cannot be ignored. This study will enable a more precise segmentation of the ribs and the development of practical deep learning algorithms.
A Lens Antenna with Reconfigurable Beams for mmWave Wind Profile Radar
Authors: Ding Yafei , Zou Ziwen , Luo Yong , Yang Guangli
Published: April 2022

Wind profile radar systems require antennas with multiple radiation beams for detecting wind velocity, as well as with a low sidelobe and dual polarization for enhancing the sensitivity for the weak signal reflected from the turbulence. This paper proposes a lens antenna operating at 24 GHz with four reconfigurable beams for wind profile radars. This lens antenna includes 2 × 2 corrugated horn antennas for radiating 24 GHz waves in two polarizations, and the dielectric lens for modulating four radiation beams with a high gain and low sidelobe. Experiments demonstrate that this lens antenna can realize reconfigurable beams with deflections of ±15° in dual polarizations, meanwhile with the gain of 30.58 dBi and the sidelobe of −20 dB. This proposed lens antenna can be applied to mmWave wind profile radars of wind turbines for enhancing wind power efficiency.
Microfluidic Thermal Flowmeters for Drug Injection Monitoring
Authors: Doh Il , Sim Daniel , Kim Steve S.
Published: April 2022

This paper presents a microfluidic thermal flowmeter for monitoring injection pumps, which is essential to ensure proper patient treatment and reduce medication errors that can lead to severe injury or death. The standard gravimetric method for flow-rate monitoring requires a great deal of preparation and laboratory equipment and is impractical in clinics. Therefore, an alternative to the standard method suitable for remote, small-scale, and frequent infusion-pump monitoring is in great demand. Here, we propose a miniaturized thermal flowmeter consisting of a silicon substrate, a platinum heater layer on a silicon dioxide thin-membrane, and a polymer microchannel to provide accurate flow-rate measurement. The present thermal flowmeter is fabricated by the micromachining and micromolding process and exhibits sensitivity, linearity, and uncertainty of 0.722 mW/(g/h), 98.7%, and (2.36 ± 0.80)%, respectively, in the flow-rate range of 0.5–2.5 g/h when the flowmeter is operated in the constant temperature mode with the channel width of 0.5 mm. The measurement range of flow rate can be easily adjusted by changing the cross-sectional microchannel dimension. The present miniaturized thermal flowmeter shows a high potential for infusion-pump calibration in clinical settings.