bluuebunny/crossref_metadata_2025 huggingface dataset:
339 Effect of DHA and EPA supplementation during the first third of gestation on growth, metabolism and gene expression in hypothalamus in finished lambs
Authors: Oviedo Mario Francisco , Roque Jose Alejandro , Lee Hector Aaron , Relling Alejandro E
Published: July 2019

Supplementation with fatty acids (FA) during late gestation regulates offspring development; however, its effect in the first third of gestation is unknown. The objective of this study was to evaluate the effects of with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplementation during the first third of gestation on productive performance and hypothalamic neuropeptides on the offspring. Seventy-nine post-weaning lambs, born of sheep supplemented in the first third of gestation with 1.5% Ca salts rich in monounsaturated FA (MUFA) or PUFA [DHA and EPA; Dam supplementation (DS)], were distributed in a 2x2 factorial arrangement of treatments to finishing diets containing MUFA or PUFA [Lamb supplementation (LS)]. The experiment last for 56 d. Measurements dry matter intake (daily), body weight and plasma metabolites (every 14 d) were taken. Twenty-four lambs were slaughtered and hypothalamus were obtained for mRNA concentration. The data were analyzed with a mixed model in SAS (9.4) using repeated measurements. There was a DS x LS interaction for BW (P < 0.10) where LS with PUFA born from DS with MUFA were heavier than the other 3 treatments. Lambs born from DS with MUFA have a greater DMI (P < 0.01). There was a time x DS effect (P < 0.05) for plasma glucose and non-esterified fatty acids (NEFA) concentration, due to a greater concentration for glucose and lower for NEFA on the lambs born from DS-MUFA at weaning but not on d 56. Lambs born from MUFA supplemented dams had a greater (P ≤ 0.05) hypothalamus mRNA concentration for CART, GH-receptor, KISS-1, leptin receptor, POMC, and NPY receptor Y1. There were no LS effects (P > 0.05) for these neuropeptides. These results indicate that supplementation with fatty acids during early pregnancy change productive performance, metabolic, and neuropeptides of lambs independently of the final diet.
345 Comparison of multiple trace mineral products provided to beef cattle as injectable, oral drench, oral paste, or bolus
Authors: Jackson Trey D , Carmichael Remy N , Deters Erin L , Messersmith Elizabeth M , VanValin Katherine , Hansen Stephanie L
Published: July 2019

To determine the effect of various trace mineral-containing products on trace mineral status of cattle 56 Angus-cross steers (303 ± 15 kg; n = 8 per treatment) were blocked by BW and administered a trace mineral treatment on d-0: injectable saline (CON), injectable Multimin®90 (ITM), MineralMax drench (MMD), MineralMax paste (MMP), Starting Fluid Drench (SFD), Se365 bolus (Se365), or Reloader250 bolus (Rel250). Steers received a common diet (silage-based d 0–49; corn-based d 50–122) via bunks capable of measuring individual feed disappearance. Plasma (0, 8, 24, and 48-h) and liver (-7, 2, 15, 29, 49, 65, 91, and 120-d) samples were obtained for analysis of Cu, Mn, Se, and Zn. Data were analyzed as repeated measures with effects of treatment, time, and treatment×time, with initial mineral concentration as covariate. Plasma Zn, Mn, and Se concentrations were affected by treatment×time (P = 0.001) where ITM was greater through 8-h for Zn and through 24-h for Mn and Se over all other treatments. Liver Se concentration was greater in ITM vs. other treatments through d-15 and Rel250 was greater than ITM and MMP on d-91 and greater than CON, MMD, MMP, and SFD on d-120 (treatment×time; P ≤ 0.001). Plasma Cu, and liver Cu, Mn, and Zn concentrations were unchanged by treatment (P ≥ 0.05). Liver Mn concentrations were least on d-2 and generally increased over time (P ≤ 0.001), while liver Zn concentrations were greatest on d-2 and least from d-29 onward (time; P ≤ 0.001). Liver Cu concentrations were greatest on d-15 and d-29 vs. other days (time; P ≤ 0.001). Overall, use of injectable trace mineral rapidly increased plasma Mn, Se and Zn, and liver Se concentrations. Oral treatments tested here exerted no effect on plasma or liver concentrations, while the multi-element bolus, Rel250, increased liver Se after 91-d.
355 Effect of shelf setting of a wet/dry feeder on growth performance of finishing pigs
Authors: Holen Julia P , Renken Molly R , Kerkaert Tim A , Boerboom Mike J , Johnston Lee J , Knott Jeffrey S
Published: July 2019

Recent advancements in wet/dry shelf feeders have caused them to become more popular in swine finishing barns. Pigs consume feed faster from wet/dry feeders compared with dry feeders, so manufacturers recommend higher stocking density than dry feeders. However, there are little data published indicating optimal feeder shelf settings for wet/dry feeders. The objective of this study was to evaluate two shelf settings for wet/dry feeders in a commercial setting. Pigs (n = 1200) were blocked by initial BW and pens were assigned within block to one of two shelf settings: 60% or 85% open. Openings for each feeder were determined by caliper measurement. Each pen (n = 20/treatment) was stocked with 30 pigs, providing 2.54 cm of linear feeder space (15 pigs per 38.1 cm feeder hole) and 0.637 m2 of floor space per pig. Mash feed (primarily corn-soybean meal-DDGS) delivery was recorded daily using Big Dutchmen Feed Exact Pro, and pigs were weighed biweekly throughout the 84 d trial. Data were analyzed using PROC MIXED of SAS with pen considered the experimental unit. Initial pig BW was similar (17.5 vs 17.4 kg; SE=0.1) for 85% and 60% treatments, respectively. Feeder shelves that were 85% open allowed pigs to have greater (P < 0.01) ADG (1.06 vs. 1.02 kg/d; SE=0.01), ADFI (2.63 vs. 2.48; SE=0.01) and were heavier at d 84 (107.1 vs 103.3 kg; SE=0.4) compared with feeder shelf openings of 60%. However, feeders that were 60% open supported better G:F (0.412 vs 0.404; P < 0.01; SE=0.001) compared with the 85% openings, respectively. No behavioral vices were observed in this study. These data suggest shelf settings for wet/dry feeders that are 85% open support faster but less efficient growth than shelf settings of 60%.
359 Determination of relative bioavailability of copper from GemStone® copper in growing beef steers
Authors: DiRusso Gabrielle C , Niedermayer Emma , Hansen Stephanie L
Published: July 2019

To evaluate bioavailability of Cu from Cu glycinate (CuGly; GemStoneCu; Phibro Animal Health Corporation) vs. Cu sulfate (CuSO4) in diets supplemented with 0.3% S and 2 mg Mo/kg DM 60 Angus-cross steers (288 ±26 kg initial BW) were randomly assigned to treatments within weight blocks and housed in pens (n = 6 steers/pen) with GrowSafe bunks. Treatments included: no supplemental Cu (CON; analyzed 6 mg Cu/kg DM); 5 mg supplemental Cu/kg DM from CuSO4 (SUL5) or CuGly (GLY5); or 10 mg supplemental Cu/kg DM from CuSO4 (SUL10) or CuGly (GLY10). Liver and blood samples were collected from all steers at the start (used as covariates) and end of the trial to determine Cu status. Steers were weighed on d 0, 28, 56, and 90. Data were analyzed as a completely randomized block design using Proc Mixed of SAS with steer as experimental unit (n = 12/treatment) with treatment and block fixed. Contrast statements compared supplementation source (sulfate vs. glycinate) and concentration. Copper supplementation did not affect steer final BW, or overall ADG, DMI, and G:F (P ≥ 0.19). Initial liver Cu concentrations (374 mg/kg DM) were highly adequate, such that even with antagonist supplementation final plasma Cu concentrations were not affected by supplemental Cu concentration (P ≥ 0.34). Control steers had lesser final liver Cu concentrations vs. steers supplemented 5 or 10 mg Cu/kg DM (P ≤ 0.0004). Supplementing 10 vs. 5 mg Cu/kg DM increased liver Cu concentrations (P = 0.0001). Source did not affect final liver Cu concentrations (P = 0.40). Relative bioavailability was determined by regressing liver Cu concentrations on Cu intake; relative bioavailability of CuGly was 115.5% compared with CuSO4 (100%; P = 0.27). Based on the calculated relative bioavailability CuGly may be a good supplemental Cu source when dietary Cu antagonists are present.
364 Impact of porcine reproductive and respiratory syndrome virus challenge and deoxynivalenol on intestinal integrity and nutrient transport
Authors: Gerrard Samuel , Helm Emma T , Todd Blaire , Gabler Nicholas K
Published: July 2019

Porcine reproductive and respiratory syndrome (PRRS) virus infections and mycotoxin contaminated feedstuffs are two common issues facing the pork industry as both act, independently, to antagonize pig intestinal function, growth performance, and health. However, the combined impact of PRRS and mycotoxin exposure on pig intestinal health and function has not been investigated. Therefore, the objective of this experiment was to examine the impact of the mycotoxin deoxynivalenol (DON) on the intestinal integrity and function of PRRS infected pigs, employing an ex vivo model. Jejunum samples were collected from PRRS naïve (control; n = 6) and 21 day post inoculated PRRS virus infected (n = 8) gilts (10 weeks of age). In duplicate, explants were mounted into modified Ussing Chambers and incubated with or without 25 mM DON for 45 minutes prior to assessment of transepithelial resistance (TER), mucosal to serosal macromolecule (FD4) flux, and active glucose and glutamine transport. Data were analyzed using the mixed model procedure of SAS and a split plot design to examine the fixed effects of PRRS, DON, and their interaction. There were no PRRS by DON interactions for any ex vivo parameters assessed. Irrespective of DON, PRRS jejunum segments had decreased TERs (57%, P < 0.001), increased FD4 flux (P = 0.005), and tended to have increased active glucose transport (P = 0.093) compared with uninfected controls. Irrespective of PRRS status, DON increased FD4 flux (113%, P = 0.013), and tended to increase active glucose transport (P = 0.088) compared with jejunal explants not treated with DON. However, TERs and active glutamine transport did not differ due to DON treatment. Taken together, these data highlight the impacts that viral health challenge and mycotoxin contamination have on aspects of intestinal integrity and function. However, it does not appear that PRRS challenged pigs are more sensitive to the effects of DON than their healthy counterparts.
363 Evaluating the effects of day of iron injection after farrowing on preweaning performance and hematological criteria
Authors: Roubicek Cierra , Williams Hayden E , DeRouchey Joel M , Woodworth Jason C , Dritz Steve S , Tokach Mike D , Goodband Robert D , Holtcamp Andrew
Published: July 2019

Newborn pigs (n = 324; 1.6 kg BW) were used in an 19-d study evaluating the effects of Fe injection timing after birth on preweaned pig performance and blood criteria. A total of 27 litters were used, with the number of pigs per sow equalized on each day of farrowing. Two d after farrowing, all piglets were weighed, and six barrows and six gilts within each litter were allotted to 1 of 6 treatments in a CRD. Treatments consisted of a negative control receiving no Fe injection or 200 mg of injectable Fe (Gleptoforte, Ceva Animal Health, Lenexa, KS) provided on d 2, 4, 6, 8, or 10 after farrowing. Piglets were weighed and bled on d 2, 12, and 21 after birth to determine growth performance and blood Fe status. Overall, increasing the age that piglets received a 200 mg Fe injection until 4 or 6 d after birth tended to increase (quadratic; P = 0.065) ADG (Table 1). Not providing an Fe injection tended to decrease (P = 0.070) overall ADG and decreased (P = 0.0003) d 21 BW compared to all other treatments. Significant treatment×day interactions (P < 0.001) were observed for hemoglobin (Hgb) and hematocrit (Hct). The interactions occurred because pigs injected with 200 mg of Fe on d 2, 4, 6, or 8 after birth had increasing values until d 21 after birth, while pigs receiving a 200 mg Fe injection on d 10 after birth had decreasing values to d 12 then increasing values to d 21. Pigs not provided an Fe injection after birth had decreasing values to d 21. These results suggest that providing a 200 mg Fe injection on d 4 or 6 after farrowing provided the greatest preweaning growth performance and blood Fe status until weaning.
365 Effect of feeding a blend of short, medium, and long chain fatty acids and triglycerides to lactating sows on piglet growth, feed intake, and survivability to weaning
Authors: Becker Larissa , Knute Nick , Abdullah Mahfuz , Dasari Mohan , Hastad Chad W
Published: July 2019

Dietary fat may impact energy intake and palatability of feed, and influence energy storage. The objective of this experiment was to test the hypothesis that including a blend of short, medium, and long chain fatty acids and triglycerides (R2 Feed Energy, Pleasant Hill, IA) in lactation diets will reduce pre-weaning mortality and increase litter weaning weight. On day 114 of gestation, dietary treatments were initiated: 1) Negative control diet and 3.2% of an all-vegetable oil blend, (Trt B); 2) Control diet with replacement of oil blend with 3.2% proprietary blend of short, medium, and long chain fatty acids and triglycerides (Trt R). Sows (n = 207; L02 Cross; PIC, Hendersonville, TN) were allotted to treatment based on body condition and parity. There were 88 parity 0 and 1 sows: 45 on TRT R and 43 on TRT B, and 119 parity 2 to 6 sows: 59 on TRT R and 60 on TRT B. Average sow parity was 2.85. Sows received 1.56 kg of their assigned diet twice per day prior to farrowing. After farrowing, sows were fed ad libitum with free access to water. Data on sow ADFI, litter weaning weight, and number of piglets weaned per sow were evaluated using PROC MIXED (SAS Institute Inc., Cary, NC). Compared with B, sows fed R consumed more feed (6.44 vs. 5.90 kg/d; P < 0.025). There was no difference in weaning weight (6.1 vs. 5.9kg; P > 0.10) or number weaned per litter (11.44 vs. 11.28; P > 0.10), for treatments R and B, respectively. There was no difference in sow body condition score after weaning (2.07 vs. 1.93; P = 0.18) for treatments R and B, respectively. The addition of R2 to sow lactation diets improved sow feed intake, but not piglet growth or number weaned.
366 Effects of organic versus conventional diets on dystocia rates in Nubian dams
Authors: Soyland Matthew J , Panetta Isabella V , Armentrout-Shoaf Bailey , Rocco Christina , Williams Toree
Published: July 2019

Organic products have been growing in popularity in the consumer market, often without evidence to support potential benefits. Many organic products promise better results than conventional products, yet research examining the use of organic products as a feedstuff in caprine diets is lacking. Additionally, the cost of organic feedstuff is much greater when compared to conventional. Reproductive success of an animal has a direct correlation with nutrition throughout pregnancy and could be impacted by organic products. Thus, potential benefits of increase of reproductive health could outweigh the initial cost of organic feedstuffs. The objective of the current study was to determine differences in dystocia occurrence between caprine dams fed conventional and organic feedstuffs in the last 6 wk of gestation. Eight gestating Nubian does (n = 4) ranging from 1 to 5 yr of age with initial BW between 45.4 and 68.5 kg were separated into two treatment groups based on BCS utilizing the American Dairy Goat Association’s BCS 1 to 5 scale. Treatment A was fed a conventional diet including 14% CP concentrate, mineral supplementation, and molasses. Treatment B was fed an equivalent USDA certified organic concentrate, mineral supplement and molasses. Dams were fed 0.907kg/d/45.4kg of BW of concentrate and mineral supplement at 0.012kg/d/45.4kg of BW in individual feeding stocks at 0630 and 1730 in the last 6 wk of gestation. All Dams were provided ad libitum to hay and water. During parturition, dystocia was defined as requiring obstetrical assistance at any point during kidding. Differences in dystocia rate between conventional and organic groups were analyzed by independent T-Test at P < 0.05. As expected, no differences were observed between organic and conventional treatment groups (p = 0.28). As a result, the increased priced of organic feedstuff was not justified for feeding over conventional products.
367 Organic versus conventional grain on placental factors in caprine
Authors: Armentrout-Shoaf Bailey , Panetta Isabella V , Soyland Matthew J , Williams Toree , Rocco Christina
Published: July 2019

Organic products are increasing in popularity due to consumer interest and animal products are no exception. Trends towards organic livestock diets have increased yet many questions exist regarding potential health benefits or negative increased cost from the organic product. Organic livestock diets could impact reproductive health yet research into organic feedstuffs is lacking. Previous research examined impacts of diet on placental factors and indicated nutrient transfer from dams to fetus. Impacts of organic practices on livestock reproduction has not been explored, as studies linking nutrition and reproductive abilities have been limited to conventional diets, despite increases in organic practices. The objective of the current study observed differences in placental factors between Nubian dams fed organic and conventional diets. Placental factors were defined as placental weight and cotyledon factors. Six (n = 3) pregnant Nubian dams were separated into an organic group and conventional group based on BW and BCS. The organic group was fed a certified organic concentrate and organic supplement, which had equivalent nutrients to conventional concentrate and supplement. Dams were fed 0.907kg/d/45.4kg of BW of concentrate and 0.012kg/d/45.4kg of BW in individual feeding stocks at 0630 and 1730 for the last six wks of gestation and three wks post-parturition. Upon expulsion of the placenta, the placenta was weighed, cotyledons were counted, and three cotyledons were measured for height, length, width, and volume. Differences in placental weight and cotyledon factors were analyzed by a two-tailed independent T-Test at P < 0.05. No differences were observed for any of the placental aspects (P = 0.486, 0.987, 0.997, 0.468, 0.448, and 0.809, respectively). Placental aspects, such as number and height, may have been influenced by the number of offspring each dam produced, but was not consistent between groups. Based upon the current study, increased cost of organic livestock production may not be justifiable for most goat producers.
371 Evaluation of parasite resistance in cattle treated with extended-release eprinomectin (LongRange)
Authors: Dawson Cody R , Volk Mareah J , Ireland Frank A , Trennepohl Keela M , McCann Joshua C , Shike Daniel W
Published: July 2019

The objective was to determine parasite resistance in cattle treated with extended-release eprinomectin (LongRange) from a herd with 3 years of prior eprinomectin use. Fall-born Angus x Simmental heifers (224 ± 22 d of age) were randomly assigned 1 of 3 treatments: LongRange (LR; n = 21), LongRange and Synanthic (COMBO; n = 21), or saline-treated control (CON; n = 21). At trial initiation, all heifers were administered treatments according to label instructions. All heifers grazed endophyte-infected tall fescue in groups (n = 3 per treatment) and supplemented daily modified wet distillers grain (0.91 kg as fed per heifer) and soybean hulls (0.91 kg as fed per heifer). Prior to trial initiation and monthly thereafter, BW, packed cell volume (PCV), and fecal egg counts (FEC) were determined. There was a treatment × date interaction (P = 0.001) for fecal eggs counts. There were no differences (P ≥ 0.53) in the FEC in May or June. In July and August, the LR and COMBO heifers had decreased (P < 0.01) FEC compared with CON. By September there were no differences (P = 0.84) in FEC. At d 28, there was no difference (P ≥ 0.44) in FEC reduction between LR (91% FEC reduction) and COMBO (98% FEC reduction). There was no treatment or treatment × date interaction (P ≥ 0.34) for PCV. There was a treatment × date interaction (P = 0.01) for BW. There were no differences (P ≥ 0.16) in BW during May, June, and July. In August the LR and COMBO heifers tended (P = 0.09) to have greater BW than CON. In September and October, the LR and COMBO heifers had a greater (P ≤ 0.01) BW than CON heifers. Parasite resistance to extended-release eprinomectin was not observed based on FEC reduction tests and similar BW of LR and COMBO heifers.
PSV-6 Assessment of lameness in gilts and barrows using gait score, force-plate, hoof hardness and hoof lesions
Authors: Wedekind Karen , LaMontagne Ashley , Foran Chelsie , Hampton Tom , Ren Ping , Chen Juxing , Vazquez-Anon Mercedes
Published: July 2019

Lameness in gilts and sows has an economic impact on pig production and is a major welfare concern. The objective of the present project was to evaluate and quantify lameness in pigs. The following methods were compared to visual gait scoring: force-plate, hoof hardness and hoof lesions. Eight barrows and seven gilts (114 ± 15 kg) were selected using visual gait scoring (0-4, 4 most severe). From this group there were 4-0’s, 4-1’s, 5-2’s, and 2-3’s. Factorial analyses, (site: front vs rear) and lameness score (0-3) and their interaction was used to evaluate effect of treatment. Consistent with published literature, rear legs were more affected by lameness than front legs (73% rear, 27% front). Only two categories for hoof lesions (white line lesion and heel erosion) were prevalent in this population. White line lesion severity increased with increasing lameness score and differed between site (P < 0.05). In contrast with literature, more white line lesions were prevalent in front vs rear. Hoof hardness was scored at 5 locations on the bottom of the hoof. At the toe tip only, there was a trend (P = 0.052) for hoof hardness to decrease with increasing lameness score, but no effect of site (P = 0.39) or interaction. Among force-plate measurements, there was a significant effect of lameness (P < 0.05) for maximum force, range (95th percentile-5th percentile), standard deviation of force, amplitude of weight-bearing (average of all positive peaks), amplitude of weight-removing (average of all negative peaks), but no significant effect observed for average force, weight shift (# of peaks/min) or contralateral ratio. On average, more weight was applied to front vs rear [30.4 vs 19.6%; (P < 0.05)]. Although further validation is warranted, these results indicate that these objective measures of lameness were sensitive enough to detect differences between lame vs non-lame pigs.
PSI-2 Impact of an extended-release dewormer on rumen VFAs and blood metabolites
Authors: Perkins Stephanie D , Rich Jerica J J , Northrop Emmalee J , Walker Julie A , Perry George A
Published: July 2019

The ratio of glucogenic to lipogenic acids in ruminal VFAs can have a direct impact on nutrient utilization. The objective of this study was to compare the effects of an extended-release injectable dewormer [LongRange® (eprinomectin)] to a traditional shorter-acting injectable dewormer [Ivomec® (doramectin)] on rumen VFA profiles and blood metabolites. Ruminally cannulated lactating cows (2yr of age) were randomly allotted to one of the two dewormers (n = 5/treatment) at the time of being moved to spring grazing (day of AI). Blood samples, rumen pH, and rumen fluid were collected prior to treatment on d 0, d 15, and approximately every 30d thereafter until d 137 and analyzed by analysis of repeated measures. Fecal samples were collected at the start, middle, and end of the study to determine parasite fecal egg count. At the initiation of the study only 3 animals had any detectable parasites by fecal egg count and at the middle of the study all animals treated with the traditional dewormer had detectable parasites. Animals treated with the extended-release dewormer had no detectable parasite load throughout the study. There was no effect of treatment or treatment by time on rumen pH (P = 0.64, 0.55) or circulating NEFA concentrations (P = 0.60, 0.65) but both changed over time (P ≤ 0.01). Circulating glucose concentrations were not affected by treatment (P = 0.71) or time (P = 0.15), but there was a treatment by time interaction (P = 0.03) with glucose concentrations increased on day 32 in the extended-release dewormer. Concentrations of rumen ammonia, total VFAs, acetate, propionate, isobutyrate, butyrate, isovalerate, and valerate were not impacted by treatment (P > 0.20), but there was an effect of time (P = 0.10). In summary, controlling parasite load during a 5mo grazing season can improve nutrient utilization in cattle.
PSVII-7 Impact of USDA feeder cattle grade and hide color on sale price of West Virginia feeder cattle
Authors: Foglesong Danielle , Shaffer Kevin
Published: July 2019

Most cow/calf operations in West Virginia are small scale (<100 cows) and calves from multiple operations must be comingled to form marketable lots. Criteria used to establish sale lots are weight, hide color, and USDA feeder cattle grade (FCG). The objective of this study was to determine the impact of hide color and FCG on calf gross sale value (GSV). Results of graded feeder calf sales from fall 2016 (N = 16) and fall 2017 (N = 18) were analyzed totaling 23,859 calves marketed as 2,507 lots. Calves were stratified by sale weight: 136-181 kg (3WT), 182-226 kg (4WT), 227-271 kg (5WT), 272-317 kh (6WT), and 318-362 kg (7WT); hide color: black (BLK) or colored (NBLK), and FCG: LM1, LM2, and non-graded consignor group (CG). Data were analyzed using the PROC GLM procedure of SAS with year and lot size (LS) as covariates. Although numerical differences in GSV existed in the 3WT, 5WT, and 6WT groups ($31.58, $15.00, $11.29/head greater for BLK calves, respectively), only 4WT BLK calves were valued significantly more ($577.22 vs $547.13, respectively; P < 0.01). No value difference was detected in 7WT calves based on hide color. With respect to FCG, no statistical difference was observed in GSV of 3WT and 6WT calves although LM1 3WT calves had a numerical value advantage of $27.73/head and $35.17/head over LM2 and CG calves, respectively, while LM1 6WT calves had a $30.05/head value advantage over LM2 6WT calves. In 4WT and 5WT classes, LM1 calves were valued higher than LM2 calves ($33.75 and $56.96/head, respectively; P < 0.01). Conversely, 7WT CG calves were valued higher than both LM1 ($888.68 vs $838.85/head, P = 0.08) and LM2 calves ($888.68 vs $799.25/head, P < 0.001). Across all weight classes, LM1 calves exhibited a numerical advantage of $37.62/head when compared to LM2 calves indicating optimum FCG is more valuable regardless of weight class. These data also indicate that hide color is less impactful on GSV as sale weight increases.
PSVII-8 Efficacy of corn dried distillers grains with solubles as a replacement for soybean meal in a Boer goat diet
Authors: Sorensen Rachel J , Stewart Savannah C , Jones Cassandra K , Crane Alison R , Nagaraja T G , Lattimer James M
Published: July 2019

Due to increased use of dried distillers grains with solubles (DDGS) in animal feed and accessibility of ethanol plants in the Midwest, this study evaluated the effect of feeding DDGS in place of soybean meal (SBM) on the fecal microbiome of Boer goats. Twenty-four Boer goat kids (apx. 70 d of age; 28.21 ± 0.96 kg) were blocked by BW and randomly assigned to 1 of 2 treatment diets for 47 d. Treatments were 0% (0DDGS) and 100% (30DDGS) DDGS in place of SBM. Goats were placed in 8 pens (4 pens/treatment; 3 goats/pen) with ad libitum access to feed and water. Fecal pellets were collected on d 47 via rectal grab and stored at -80°C until microbiome sequencing was performed. The V4 region of the 16S rRNA gene was sequenced by MR DNA (MR DNA, Shallowater, TX) on the Illumina HiSeq 2500 platform (Illumina, Inc., San Diego, CA). Data were analyzed using ANOVA with Tukey’s test for pairwise comparisons. Genera impacted by DDGS inclusion with individual relative abundances greater than 1% included increased Ruminococcus (P = 0.01) and Methanobrevibacter (P = 0.009) and decreased Lachnoclostridium (P = 0.02). Ruminococcus and Methanobrevibacter most likely increased in 30DDGS due to greater amounts of soluble fiber passing through the rumen, thus being fermented in the hindgut. The overall percentage of the phyla Bacteroidetes (P = 0.36) and Firmicutes (P = 0.12) did not differ between treatments; however, Firmicutes:Bacteroidetes increased (P = 0.05) in the 30DDGS diet. Treatment did not impact β-diversity (P = 0.47) although species richness increased (P = 0.09) in DDGS-fed goats as more soluble fiber was available for fermentation in the hindgut. In all, results of this study found replacing SBM with DDGS did not greatly alter the fecal microbiome of Boer goats.
PSIII-13 Genetic parameters and the purebred–crossbred genetic correlation for growth, carcass, and meat quality traits in pigs
Authors: Esfandyari Hadi , Thekkoot Dinesh M , Kemp Robert A , Plastow Graham S , Dekkers Jack C M
Published: July 2019

Growth, meat quality, and carcass traits are of economic importance in swine breeding. Understanding their genetic basis in commercial crossbred pigs and purebred-crossbred genetic correlations are necessary for a successful breeding program. The objectives of this study were to 1) estimate genetic parameters for growth, meat quality and carcass traits in a purebred sire line and related commercial crossbred pigs and 2) estimate the corresponding genetic correlations between purebreds and crossbreds (rpc). We analyzed 115266, 10927 and 43057 purebred records for growth, meat quality (n = 4) and carcass traits (n = 7), respectively. For crossbreds, there were 2000 pigs with growth records, with 900 of them having meat quality and carcass data. A series of univariate and bivariate analyses were used to estimate genetic parameters and rpc. Growth showed moderate heritability (0.20 ± 0.10 to 0.25 ± 0.01) in both purebreds and crossbreds. Heritability estimates for meat quality traits ranged from 0.21 ± 0.03 to 0.42 ± 0.04 in purebreds and from 0.17 ± 0.14 to 0.47 ± 0.15 in crossbreds. Carcass traits had higher heritability estimates in purebreds compared to crossbreds, except for hot carcass weight (0.10 ± 0.02 vs. 0.24 ± 0.16). Genetic correlations among meat quality traits were variable in both populations, whereas genetic correlations among carcass traits were similar in purebreds and crossbreds. Estimates of rpc were high for growth (0.99 ± 0.5) and for meat quality traits (0.94 ± 0.39 to 0.99 ± 0.2), except for Minolta color (-0.48 ± 0.56). Carcass traits had moderate to high estimates of rpc (0.64 ± 0.4 to 0.92 ± 0.3). Carcass fat had a negative estimate of rpc (-0.1 ± 0.5). However, ultrasound fat as an indicator trait for carcass fat had a high positive estimate of rpc (0.88 ± 0.14). Our results indicate that selection in purebreds can be efficient to improve these traits in both purebreds and crossbreds but for some traits, genetic gain can be improved by applying combined crossbred and purebred selection. Funding provided by Genome Canada and the National Research Council.
PSIII-14 Performance differences between two groups of commercial pigs following experimental infection with porcine reproductive and respiratory syndrome virus (PRRS) virus 1-7-4
Authors: Little Erin , Dunkelberger Jenelle , Hanson Dan , Eggert John , Gonda Michael , MacNeil Michael , Dee Scott
Published: July 2019

Porcine reproductive and respiratory syndrome virus (PRRSv) is the costliest swine disease of North America. Vaccines and management alone have not been effective at controlling this disease and genetic selection may be a complimentary approach to controlling PRRSv-infection. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSv 1-7-4: 1) pigs sired by boars selected based on a standard index (STD), which emphasizes feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (EXP), with emphasis on feed intake, piglet vitality, and robustness. Pigs (n~730 per group) were housed in a commercial research wean-to-finish barn. Average daily gain (ADG) and average daily feed intake (ADFI) were recorded from birth-to-slaughter. At weaning, pigs were placed in pens by group and balanced by sex. To facilitate collection of feed intake data, the experimental unit was pen, with 27 pens representing each group. Four weeks post-wean, all pigs were experimentally infected with 2mL of 1-7-4 PRRSv at 3.5 logs of TCID50 per mL. Statistical analyses were performed using a linear mixed model with group (STD or EXP) as a fixed effect and block as a random effect. No significant difference was detected between groups for wean-to-finish or 0 to 42 days post-infection (dpi) ADG. The ADFI was 0.06 kg/day greater for the EXP group than the STD group from 0 to 42 dpi (P = 0.01). Feed conversion ratio (FCR) was 0.03 (P = 0.03) less for the EXP group from wean-to-finish and 0 to 42 dpi, respectively. Pigs sired by boars selected using the experimental index showed no significant difference in ADG, but had greater ADFI post-challenge, while pigs sired by boars selected based on the standard index had significantly better FC.
PSIII-4 Preliminary exploration of relationship of automated sensor data with feed intake and efficiency in lactating dairy cattle
Authors: Siberski Cori J , Goetz Brady M , Baumgard Lance H , Koltes James E
Published: July 2019

Feed costs represent the greatest expense on a dairy farm, making feed efficiency an important trait to consider among production traits. Current tools to measure feed intake have limited application in commercial settings, due to affordability and lack of portability of technologies. Therefore, development of automated sensor-based indicator traits for feed intake could prove to be valuable. The objective of the current study was to determine if automated eartag data was associated with feed intake. Activity and inner ear temperature were collected every 19 minutes utilizing Quantified Ag eartags (n = 48 lactating cows). Ear tags were placed 5 days prior to the start of the trial, with cows ranging from 67-192 days in milk (DIM). Daily feed intake, milk weights, milk components and body weight (BW) were also recorded. Data were analyzed using PROX GLIMMIX in SAS. Dry matter intake (DMI) was modeled including fixed effects for DIM, milk weight, component composition, metabolic body weight (BW0.75), eartag activity or temperature, as well as the random effects of parity and group. To identify informative timeframes with reduced influence of environmental noise, data were analyzed over 3-day rolling windows of time. Six windows were significantly associated with dry matter intake (P ≤ 0.05) when utilizing ear tag activity. Three windows of time of ear tag temperature were found to be significantly associated with DMI (P ≤ 0.05). These findings indicate that eartag sensor data may be useful indicators of feed intake; however, days in milk and season may impact the informativeness of sensor data. Additional studies are warranted to validate the efficacy of activity and ear temperature as indicators of feed intake and determine the impact of other variables on these potential sensor indicator traits over time.
PSIII-5 Accuracy of genomic prediction of antibody response to common infectious diseases in commercial sows
Authors: Sanglard Leticia Pereira , Canada PigGen , Mote Benny , Willson Philip , Harding John C S , Plastow Graham S , Dekkers Jack C M , Serão Nick V L
Published: July 2019

Previous results indicated that antibody response to PRRSV has moderate genomic prediction accuracy; however, little is known about this for other common infectious diseases. Therefore, the objective of this study was to estimate the accuracy of genomic prediction for antibody response to infectious diseases in commercial sows. A total of 2,848 Large White x Landrace replacement gilts were sourced from 17 high-health multipliers (7 breeding companies; BC) and introduced to 23 commercial farms with a history of common diseases, following standard acclimation procedures. Serum was used to quantify antibody response to swine influenza virus (SIV), Mycoplasma hyopneumoniae (MH), porcine circovirus type 2 (PCV2), and 8 serotypes of Actinobacilluspleuropneumoniae(APP1-3, 5, 7, 10, 12, and 13) at entry (S/PEntry), following acclimation (S/PAcclimation), and during parities 1 (S/PParity1) and 2 (S/PParity2). All animals were genotyped for 38,191 SNPs. Genomic prediction was performed using BayesB (pi=0.99), with the fixed effect of CG and random effects of SNPs included in the model. Training and validation were performed using 7-fold cross-validation, with data from each BC used as the validation dataset in one-fold. In general, prediction accuracies were low: SIV, from 0.13 (S/PAcclimation) to 0.26 (S/PParity1); MH, -0.07 (S/PAcclimation) to 0.13 (S/PParity2); PCV2, 0.04 (S/PParity1) to 0.32 (S/PAcclimation); APP, -0.08 (S/PEntry, APP10) to 0.26 (S/PAcclimation, APP7). At each point, average accuracies were 0.06 for S/PEntry, 0.09 for S/PAcclimationand S/PParity1, and 0.08 for S/PParity2, showing small increases in accuracy after the acclimation period. Among diseases, average accuracies ranged from 0.01 (APP1) to 0.22 (PCV2). Results show that, overall, the accuracy of genomic prediction of antibody response to common infectious diseases in commercial gilts is limited. The authors thank PigGen Canada, Genome Canada, and the Canadian Swine Health Board for financial support, and the late Dr. Stephen Bishop for his scientific contributions.
PSVII-3 Effects of oral administration of essential oils on anti-immune stress, antimicrobial and repairing the intestinal damage in broilers challenged by lipopolysaccharide
Authors: Oh Hanjin , Liu Shudong , Yun Won , Lee Jihwan , An Jiseon , Cho Jinho
Published: July 2019

The aim of the present study was to evaluate the effects of oral administration of essential oils (Carvacrol, Thyme and Oregano essential oils) on anti-immune stress, antimicrobial and repairing the intestinal damage, caused by Salmonella enterica Lipopolysaccharide, in vivo of broilers. A total of 100 ROSS 308 broilers (21-day-old; 1.10 ± 0.11kg) were randomly allocated to 5 groups, T1: basal diet + Saline; T2: basal diet + LPS; T3: basal diet+ 200μL carvacrol oils+ LPS; T4: basal diet+ 200μL thyme oils+ LPS; T5: basal diet+ 200μL oregano oils+ LPS, with 20 replicates every group, and 1 chicken per replicate per cage. The challenged by LPS resulted in an immune stress, which manifests as the abnormal growth (P < 0.05) in immune organs, the content of IgG (P < 0.05) and TNF-α(P < 0.05) and the rectum temperature (P < 0.05) increased compared with other groups; the oral administration of essential oils controlled the immune stress to a certain extent. The essential oils could withhold maleficent bacteria, as E. coli (P < 0.05) and Salmonella enumeration (P < 0.05), in vivo of the broiler. Meanwhile, the essential oils repaired the intestinal damage which shown a reduction in the villi height (P < 0.05) and goblet cell (P < 0.05) caused by LPS. In conclusion, the essential oils (Carvacrol, Thyme and Oregano essential oils) controlled the stress reaction and maintain intestinal health to a certain extent. http://www.conferenceharvester.com/
PSIII-16 The effect of Activo and a competitor phytogenic on growth performance and carcass characteristics of grow-finish pigs
Authors: Cabrera Rafael A
Published: July 2019

The objective of this study was to evaluate the effects of feeding two phytogenics compunds on growth performance and carcass characteristics in grow-finish pigs. Activo is a phytogenic feed additive consisting of a blend of microencapsulated secondary plant compounds used as an alternative to antibniotics to support gut health and overall animal performance. The study was desgined as a split plot design with 2 treatments (Activo and Control) with 44 replicates each and 22 pigs per pen. Pigs were alloted by weight (41.9 kgs in average) and age (about 12 weeks of age). Three dietary feeding phases were used. Grow-finish phase 1 (9-16 weeks post weaning) containing 13.6 g/ton of Skycis plus the tested phytogenics; grow-finish phase 2 had the tested phytogenics and finally gorw-finish phase 3 had 6.75 g/ton of Ractopamine plus the tested phytogenics. There were no siginifcant differences between the two groups on overal growth performance and mortality. Carcass characteristics data was analyzed by combining harvest cuts 1 and 2. Cut 1 was performed 15 days after the supplementation of Ractopamine and cut 2 when the barn was empty. This includes 27 pens for the Activo group and 30 pens for the Control group due to carcass data that was removed or missing. Any pen with a harvest cut missing 3 or more carcasses was removed from the data set. The Acitvo group had heavier (P = 0.03) hot carcass weigh (101.7 vs. 100.3 kgs, respectively) and higher (P = 0.04) carcass yield % (76.3 vs. 75.8%, respectively) when compared to the Control group. There was a tendency for the Activo group to have higher (P = 0.06) Longissimus muscel depth than the Control group. In conclusion, the Activo group appears to be a better alternative to antibiotics than the competitor phytogenic.
PSIII-21 Processing of food proteins of animal origin sometimes, but not always, increases values for Digestible Indispensable Amino Acid Scores
Authors: Bailey Hannah M , Berg Eric P , Stein Hans H
Published: July 2019

Two experiments were conducted to test the hypothesis that processing of meat proteins increases the Digestible Indispensable Amino Acid Score (DIAAS). In Exp. 1, 10 pigs (26.62 ± 1.62 kg) with a T-cannula in the distal ileum were randomly allotted to a 10 x 10 Latin square with 10 diets and ten 7-d periods. In Exp. 2, 9 cannulated pigs (35.50 ± 3.77 kg) were randomly allotted to a 9 x 8 Youden square with 9 diets and eight 7-d periods. Meat proteins (Table 1) were included in the diet as the only source of AA. The DIAAS for each meat protein was calculated using the Food and Agriculture Organization of the United Nations (FAO) established reference patterns for children 6 mo to 3 yr and older children, adolescents, and adults. Data were analyzed using PROC MIXED of SAS in a randomized complete block design with the pig as the experimental unit. In Exp. 1, all meat proteins had a DIAAS greater than 100% regardless of the reference pattern. For both reference patterns, the DIAAS for smoked-cooked bacon was greater (P < 0.05) than for raw belly and smoked bacon, the 2 cured hams had a numerically greater DIAAS than the uncured ham, and the medium loin had a greater (P < 0.05) DIAAS compared with the medium-well and well-done loins. In Exp. 2, most proteins had a DIAAS value greater than 100%. For both reference patterns, raw ground beef had a greater (P < 0.05) DIAAS than cooked ground beef, and medium ribeye roast had a greater (P < 0.05) DIAAS than medium-rare and well-done ribeye roasts. In conclusion, curing and moderate cooking may increase DIAAS, whereas grinding meat prior to some, but not all, cooking methods or overcooking may reduce the DIAAS of meat proteins.
PSIII-20 Oil source and peroxidation affects performance and vitamin E status in nursery pigs
Authors: Lindblom Stephanie , Faris Richard , Zhao Junmei , Kerr Brian
Published: July 2019

Oil source and peroxidation are known to impact performance and oxidative status in young pigs. The objectives of this trial were to evaluate the effect of oil source and peroxidation status on pig performance and plasma vitamin E. Pigs (initial BW 6.07 ± 0.12 kg) were allotted to 96 pens containing 3 pigs/pen in a completely randomized design and fed for 21 d. The 4 × 2 factorial arrangement of treatments included 4 oil sources (palm, poultry fat, soybean, and canola oil) and either fresh (FO) or peroxidized oil (PO). Peroxidation was achieved by heating oils at 90oC for 72 h with air flow rate of 12L/min. Both FO and PO were analyzed for peroxide value and anisidine value, each of which increased with peroxidation compared to FO, while peroxidation reduced total tocopherols. Blood was also collected from one randomly selected pig/pen from 9 replications per treatment on d 12 for plasma vitamin E analysis. PO decreased both ADFI (318 vs. 338 g, P < 0.05) and ADG (267 vs. 289 g, P < 0.05) compared to FO. No difference in ADG and ADFI was detected for the main effect of oil source. An interaction tended to occur (P = 0.10) for ADFI where pigs fed PO palm, soybean, and canola oil had reduced ADFI compared to pigs fed peroxidized poultry fat, with the greatest reduction in ADFI noted for pigs fed peroxidized soybean and canola oil. Changes in ADG were directionally similar. Plasma vitamin E was reduced in pigs fed PO (P < 0.01), with the largest difference in pigs fed peroxidized soybean (-67%) and canola oil (-40%) compared to fresh soybean and canola oil. Based on changes in peroxidation status, performance and plasma vitamin E were reduced most in pigs fed soybean and canola oils and less in pigs fed palm oil and poultry fat.
PSIII-22 Performance of DNA 600 Duroc sired pigs when split sex fed with commercial diets with or without a blend of phytonutrients (Lean Fuel)
Authors: Soltwedel Kevin T , England Steve J , Sandberg Fredrik , Bible Megan R
Published: July 2019

The objective was to evaluate the effect of a blend of phytonutrients (Lean Fuel, LF) on performance of pigs in late finishing in a commercial research barn. A total of 590 DNA 600 Duroc finishing pigs (BW=75.7 + 1.0 kg) were blocked by weight and sex and allocated across two dietary treatments with 6 replications per treatment and 21 to 26 pigs per pen. Dietary treatments were: barrow (B) diets with and without LF and gilt (G) diets with and without LF. Diets were formulated to split-sex requirements (CON) for each period and LF diets were control diets + 0.125% LF. The experiment was 44-d where d 0 was 98-d post-wean. All data were analyzed using the MIXED procedure of SAS as a randomized complete block design as a 2 x 2 (diet x sex) factorial arrangement. Pen served as the experimental unit. Overall (d 0-44), the B gained more weight (P = 0.0282) and consumed more feed (P > 0.10) in ADFI. There was no diet x sex interaction (P > 0.10) for ADG. There was a tendency for an interaction (P = 0.0545) for ADFI, where LF B consumed more feed compared to CON B and CON G consumed more feed compared to LF G. There was an interaction for G:F (P = 0.0028) where G on LF had higher G:F compared to G on CON whereas G:F for B was not different to B on LF. In conclusion, LF improved ADG and G:F, but did so differently for G and B.

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