bluuebunny/crossref_metadata_2025 huggingface dataset:
Two tripartite classification systems of CD86+ and CD206+ macrophages are significantly associated with tumor recurrence in stage II-III colorectal cancer
Authors: Xu Guozeng , Mo Yuzhen , Li Jing , Wei Qingqing , Zhou Fuxiang , Chen Jian
Published: June 2023

Introduction

The prognostic value of tumor-associated macrophages remains unclear in colorectal cancer (CRC). Two tripartite classification systems, namely, ratio and quantity subgroups, were investigated as the prognostic stratification tools for stage II-III CRC.

Methods

We assessed the infiltration intensity of CD86+ and CD206+ macrophages in 449 cases with stage II-III disease by immunohistochemical staining. Ratio subgroups were defined by the lower- and upper-quartile points of CD206+/(CD86++CD206+) macrophage ratio, including the low-, moderate-, and high-ratio subgroups. Quantity subgroups were defined by the median points of CD86+ and CD206+ macrophages and included the low-, moderate-, and high-risk subgroups. The main analysis was recurrence-free survival (RFS) and overall survival (OS).

Results

Ratio subgroups (RFS/OS: HR=2.677/2.708, all p<0.001) and quantity subgroups (RFS/OS: HR=3.137/3.250, all p<0.001) could serve as independent prognostic indicators that effectively predicted survival outcomes. More importantly, log-rank test revealed that patients in the high-ratio (RFS/OS: HR=2.950/3.151, all p<0.001) or high-risk (RFS/OS: HR=3.453/3.711, all p<0.001) subgroup exhibited decreased survival outcomes after adjuvant chemotherapy. The predictive accuracy of the quantity subgroups within 48 months was higher than that of the ratio subgroups and tumor stage (all p<0.05).

Conclusions

Ratio and quantity subgroups could serve as independent prognostic indicators that could potentially be incorporated into the tumor staging algorithm to improve prognostic stratification and provide better predictions of survival outcomes in stage II-III CRC after adjuvant chemotherapy.
The gastrointestinal-brain-microbiota axis: a promising therapeutic target for ischemic stroke
Authors: Wei Yan-hao , Bi Ren-tang , Qiu Yan-mei , Zhang Chun-lin , Li Jian-zhuang , Li Ya-nan , Hu Bo
Published: June 2023

Ischemic stroke is a highly complex systemic disease characterized by intricate interactions between the brain and gastrointestinal tract. While our current understanding of these interactions primarily stems from experimental models, their relevance to human stroke outcomes is of considerable interest. After stroke, bidirectional communication between the brain and gastrointestinal tract initiates changes in the gastrointestinal microenvironment. These changes involve the activation of gastrointestinal immunity, disruption of the gastrointestinal barrier, and alterations in gastrointestinal microbiota. Importantly, experimental evidence suggests that these alterations facilitate the migration of gastrointestinal immune cells and cytokines across the damaged blood-brain barrier, ultimately infiltrating the ischemic brain. Although the characterization of these phenomena in humans is still limited, recognizing the significance of the brain-gastrointestinal crosstalk after stroke offers potential avenues for therapeutic intervention. By targeting the mutually reinforcing processes between the brain and gastrointestinal tract, it may be possible to improve the prognosis of ischemic stroke. Further investigation is warranted to elucidate the clinical relevance and translational potential of these findings.
Personalized monitoring of circulating tumor DNA with a specific signature of trackable mutations after chimeric antigen receptor T-cell therapy in follicular lymphoma patients
Authors: Jiménez-Ubieto Ana , Martín-Muñoz Alejandro , Poza María , Dorado Sara , García-Ortiz Almudena , Revilla Enrique , Sarandeses Pilar , Ruiz-Heredia Yanira , Baumann Tycho , Rodríguez Antonia , Calbacho María , Sánchez Pilar Martínez , Pina José María Sánchez , García-Sancho Alejandro Martín , Figaredo Gloria , Gil-Alós Daniel , Rufián Laura , Rodríguez Margarita , Carneros Laura , Martínez-Laperche Carolina , Bastos-Oreiro Mariana , Wang Chongwu , Cedena María-Teresa , Rapado Inmaculada , de Toledo Paula , Gallardo Miguel , Valeri Antonio , Ayala Rosa , Martínez-López Joaquín , Barrio Santiago
Published: June 2023

Background

CART therapy has produced a paradigm shift in the treatment of relapsing FL patients. Strategies to optimize disease surveillance after these therapies are increasingly necessary. This study explores the potential value of ctDNA monitoring with an innovative signature of personalized trackable mutations.

Method

Eleven FL patients treated with anti-CD19 CAR T-cell therapy were included. One did not respond and was excluded. Genomic profiling was performed before starting lymphodepleting chemotherapy to identify somatic mutations suitable for LiqBio-MRD monitoring. The dynamics of the baseline mutations (4.5 per patient) were further analyzed on 59 cfDNA follow-up samples. PET/CT examinations were performed on days +90, +180, +365, and every six months until disease progression or death.

Results

After a median follow-up of 36 months, all patients achieved a CR as the best response. Two patients progressed. The most frequently mutated genes were CREBBP, KMT2D and EP300. Simultaneous analysis of ctDNA and PET/CT was available for 18 time-points. When PET/CT was positive, two out of four ctDNA samples were LiqBio-MRD negative. These two negative samples corresponded to women with a unique mesenteric mass in two evaluations and never relapsed. Meanwhile, 14 PET/CT negative images were mutation-free based on our LiqBio-MRD analysis (100%). None of the patients had a negative LiqBio-MRD test by day +7. Interestingly, all durably responding patients had undetectable ctDNA at or around three months after infusion. Two patients presented discordant results by PET/CT and ctDNA levels. No progression was confirmed in these cases. All the progressing patients were LiqBio-MRD positive before progression.

Conclusion

This is a proof-of-principle for using ctDNA to monitor response to CAR T-cell therapy in FL. Our results confirm that a non-invasive liquid biopsy MRD analysis may correlate with response and could be used to monitor response. Harmonized definitions of ctDNA molecular response and pinpointing the optimal timing for assessing ctDNA responses are necessary for this setting. If using ctDNA analysis, we suggest restricting follow-up PET/CT in CR patients to a clinical suspicion of relapse, to avoid false-positive results.
Do whales really increase the oceanic removal of atmospheric carbon?
Authors: Meynecke Jan-Olaf , Samanta Saumik , de Bie Jasper , Seyboth Elisa , Prakash Dey Subhra , Fearon Giles , Vichi Marcello , Findlay Ken , Roychoudhury Alakendra , Mackey Brendan
Published: June 2023

Whales have been titled climate savers in the media with their recovery welcomed as a potential carbon solution. However, only a few studies were performed to date providing data or model outputs to support the hypothesis. Following an outline of the primary mechanisms by which baleen whales remove carbon from the atmosphere for eventual sequestration at regional and global scales, we conclude that the amount of carbon whales are potentially sequestering might be too little to meaningfully alter the course of climate change. This is in contrast to media perpetuating whales as climate engineers. Creating false hope in the ability of charismatic species to be climate engineers may act to further delay the urgent behavioral change needed to avert catastrophic climate change impacts, which can in turn have indirect consequences for the recovery of whale populations. Nevertheless, whales are important components of marine ecosystems, and any further investigation on existing gaps in their ecology will contribute to clarifying their contribution to the ocean carbon cycle, a major driver of the world’s climate. While whales are vital to the healthy functioning of marine ecosystems, overstating their ability to prevent or counterbalance anthropogenically induced changes in global carbon budget may unintentionally redirect attention from known, well-established methods of reducing greenhouse gases. Large scale protection of marine environments including the habitats of whales will build resilience and assist with natural carbon capture.
Coupled magnetic Mie resonances induced extraordinary optical transmission and its non-linear tunability
Authors: Cai Weiqi , Xuan Lixin , Fan Yuancheng , Yang Ruisheng , Zhang Wenwu , Zhang Qing , Chen Songnan , Ye Zhehao , Zhang Yujing , Fu Quanhong , Zhang Fuli
Published: June 2023

Dielectric metamaterials with low ohmic losses and resonating in the local magnetic mode are preferable for enhancing material non-linearity. Here, we propose and experimentally demonstrate broadband extraordinary electromagnetic transmission (EET) behavior, which is induced by the coupling of magnetic modes of two ceramic cuboids. It is shown that extraordinary electromagnetic transmission behavior through a perforated metal sheet with a subwavelength aperture can be achieved by exciting the first-order magnetic mode Mie resonant coupling of these cuboids. Our findings indicate that the transmission bandwidth and amplitude are dependent on the strength of coupling between the two ceramic cuboids. Additionally, we utilized non-linear effects within the dielectric cuboids to achieve tunable extraordinary electromagnetic transmission behavior. Our results are promising for developing non-linearly tunable microwave devices such as filters and modulators of their strong light-matter interactions.
Novel protein biomarkers for pneumonia and acute exacerbations in COPD: a pilot study
Authors: Jung Anna Lena , Han Maria , Griss Kathrin , Bertrams Wilhelm , Nell Christoph , Greulich Timm , Klemmer Andreas , Pott Hendrik , Heider Dominik , Vogelmeier Claus F. , Hippenstiel Stefan , Suttorp Norbert , Schmeck Bernd
Published: June 2023

Introduction

Community-acquired pneumonia (CAP) and acute exacerbations of chronic obstructive pulmonary disease (AECOPD) result in high morbidity, mortality, and socio-economic burden. The usage of easily accessible biomarkers informing on disease entity, severity, prognosis, and pathophysiological endotypes is limited in clinical practice. Here, we have analyzed selected plasma markers for their value in differential diagnosis and severity grading in a clinical cohort.

Methods

A pilot cohort of hospitalized patients suffering from CAP (n = 27), AECOPD (n = 10), and healthy subjects (n = 22) were characterized clinically. Clinical scores (PSI, CURB, CRB65, GOLD I-IV, and GOLD ABCD) were obtained, and interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-2-receptor (IL-2R), lipopolysaccharide-binding protein (LBP), resistin, thrombospondin-1 (TSP-1), lactotransferrin (LTF), neutrophil gelatinase-associated lipocalin (NGAL), neutrophil-elastase-2 (ELA2), hepatocyte growth factor (HGF), soluble Fas (sFas), as well as TNF-related apoptosis-inducing ligand (TRAIL) were measured in plasma.

Results

In CAP patients and healthy volunteers, we found significantly different levels of ELA2, HGF, IL-2R, IL-6, IL-8, LBP, resistin, LTF, and TRAIL. The panel of LBP, sFas, and TRAIL could discriminate between uncomplicated and severe CAP. AECOPD patients showed significantly different levels of LTF and TRAIL compared to healthy subjects. Ensemble feature selection revealed that CAP and AECOPD can be discriminated by IL-6, resistin, together with IL-2R. These factors even allow the differentiation between COPD patients suffering from an exacerbation or pneumonia.

Discussion

Taken together, we identified immune mediators in patient plasma that provide information on differential diagnosis and disease severity and can therefore serve as biomarkers. Further studies are required for validation in bigger cohorts.
Decoding the genetic relationship between Alzheimer’s disease and type 2 diabetes: potential risk variants and future direction for North Africa
Authors: Boukhalfa Wided , Jmel Haifa , Kheriji Nadia , Gouiza Ismail , Dallali Hamza , Hechmi Mariem , Kefi Rym
Published: June 2023

Introduction

Alzheimer’s disease (AD) and Type 2 diabetes (T2D) are both age-associated diseases. Identification of shared genes could help develop early diagnosis and preventive strategies. Although genetic background plays a crucial role in these diseases, we noticed an underrepresentation tendency of North African populations in omics studies.

Materials and methods

First, we conducted a comprehensive review of genes and pathways shared between T2D and AD through PubMed. Then, the function of the identified genes and variants was investigated using annotation tools including PolyPhen2, RegulomeDB, and miRdSNP. Pathways enrichment analyses were performed with g:Profiler and EnrichmentMap. Next, we analyzed variant distributions in 16 worldwide populations using PLINK2, R, and STRUCTURE software. Finally, we performed an inter-ethnic comparison based on the minor allele frequency of T2D-AD common variants.

Results

A total of 59 eligible papers were included in our study. We found 231 variants and 363 genes shared between T2D and AD. Variant annotation revealed six single nucleotide polymorphisms (SNP) with a high pathogenic score, three SNPs with regulatory effects on the brain, and six SNPs with potential effects on miRNA-binding sites. The miRNAs affected were implicated in T2D, insulin signaling pathways, and AD. Moreover, replicated genes were significantly enriched in pathways related to plasma protein binding, positive regulation of amyloid fibril deposition, microglia activation, and cholesterol metabolism. Multidimensional screening performed based on the 363 shared genes showed that main North African populations are clustered together and are divergent from other worldwide populations. Interestingly, our results showed that 49 SNP associated with T2D and AD were present in North African populations. Among them, 11 variants located in DNM3, CFH, PPARG, ROHA, AGER, CLU, BDNF1, CST9, and PLCG1 genes display significant differences in risk allele frequencies between North African and other populations.

Conclusion

Our study highlighted the complexity and the unique molecular architecture of North African populations regarding T2D-AD shared genes. In conclusion, we emphasize the importance of T2D-AD shared genes and ethnicity-specific investigation studies for a better understanding of the link behind these diseases and to develop accurate diagnoses using personalized genetic biomarkers.
Regulation of enteric nervous system via sacral nerve stimulation in opioid-induced constipated rats
Authors: Wang Liyun , Gharibani Payam , Yang Yi , Guo Yu , Yin Jieyun
Published: June 2023

Objectives

Sacral nerve stimulation (SNS) has been employed for treating constipation. However, its mechanisms involving enteric nervous system (ENS) and motility are largely unknown. In this study, we investigated the possible ENS involvement of SNS in treating Loperamide-induced constipation in rats.

Methods

Experiment-1 was designed to study the effects of acute SNS on whole colon transit time (CTT). In experiment-2, we induced constipation by Loperamide and then applied daily SNS or sham-SNS for 1 week. Choline acetyltransferase (ChAT), nitric oxide synthase (nNOS), and PGP9.5 in colon tissue were examined at the end of the study. Moreover, survival factors such as phosphorylated AKT (p-AKT) and Glial cell-derived neurotrophic factor (GDNF) were measures by immunohistochemistry (IHC) and western blot (WB).

Key results

(1) SNS with one set of parameters shortened CTT starting at 90 min after phenol red administration (p < 0.05). (2) While Loperamide induced slow transit constipation with a significant reduction in fecal pellet number and feces wet weight, daily SNS for a week resolved constipation. (3) Moreover, SNS was able to shorten whole gut transit time comparing to sham-SNS (p = 0.01). (4) Loperamide reduced the number of PGP9.5 and ChAT positive cells, and downregulated ChAT protein expression and upregulated nNOS protein expression, whereas these detrimental effects were significantly reversed by SNS. (5) Furthermore, SNS increased expressions of both GDNF and p-AKT in colon tissue. (6) Vagal activity was reduced following Loperamide (p < 0.01); yet SNS normalized vagal activity.

Conclusion

SNS with appropriate parameters improves opioid-induced constipation and reversed the detrimental effects of Loperamide on enteric neurons possibly via the GDNF-PI3K/Akt pathway.

GRAPHICAL ABSTRACT
Impaired large-scale cortico–hippocampal network connectivity, including the anterior temporal and posterior medial systems, and its associations with cognition in patients with first-episode schizophrenia
Authors: Xue Kangkang , Chen Jingli , Wei Yarui , Chen Yuan , Han Shaoqiang , Wang Caihong , Zhang Yong , Song Xueqin , Cheng Jingliang
Published: June 2023

Background and objective

The cortico–hippocampal network is an emerging neural framework with striking evidence that it supports cognition in humans, especially memory; this network includes the anterior temporal (AT) system, the posterior medial (PM) system, the anterior hippocampus (aHIPPO), and the posterior hippocampus (pHIPPO). This study aimed to detect aberrant patterns of functional connectivity within and between large-scale cortico–hippocampal networks in first-episode schizophrenia patients compared with a healthy control group via resting-state functional magnetic resonance imaging (rs-fMRI) and to explore the correlations of these aberrant patterns with cognition.

Methods

A total of 86 first-episode, drug-naïve schizophrenia patients and 102 healthy controls (HC) were recruited to undergo rs-fMRI examinations and clinical evaluations. We conducted large-scale edge-based network analysis to characterize the functional architecture of the cortico–hippocampus network and investigate between-group differences in within/between-network functional connectivity. Additionally, we explored the associations of functional connectivity (FC) abnormalities with clinical characteristics, including scores on the Positive and Negative Syndrome Scale (PANSS) and cognitive scores.

Results

Compared with the HC group, schizophrenia patients exhibited widespread alterations to within-network FC of the cortico–hippocampal network, with decreases in FC involving the precuneus (PREC), amygdala (AMYG), parahippocampal cortex (PHC), orbitofrontal cortex (OFC), perirhinal cortex (PRC), retrosplenial cortex (RSC), posterior cingulate cortex (PCC), angular gyrus (ANG), aHIPPO, and pHIPPO. Schizophrenia patients also showed abnormalities in large-scale between-network FC of the cortico–hippocampal network, in the form of significantly decreased FC between the AT and the PM, the AT and the aHIPPO, the PM and the aHIPPO, and the aHIPPO and the pHIPPO. A number of these signatures of aberrant FC were correlated with PANSS score (positive, negative, and total score) and with scores on cognitive test battery items, including attention/vigilance (AV), working memory (WM), verbal learning and memory (Verb_Lrng), visual learning and memory (Vis_Lrng), reasoning and problem-solving (RPS), and social cognition (SC).

Conclusion

Schizophrenia patients show distinct patterns of functional integration and separation both within and between large-scale cortico–hippocampal networks, reflecting a network imbalance of the hippocampal long axis with the AT and PM systems, which regulate cognitive domains (mainly Vis_Lrng, Verb_Lrng, WM, and RPS), and particularly involving alterations to FC of the AT system and the aHIPPO. These findings provide new insights into the neurofunctional markers of schizophrenia.
Survival status and predictors of mortality from severe community-acquired pneumonia among under-five children admitted at Debre Tabor comprehensive specialized hospital: a prospective cohort study
Authors: Kassaw Amare , Kerebih Gashaw , Zeleke Shegaw , Chanie Ermias Sisay , Dessalegn Nigatu , Bante Berihun , Teshome Asefa Ageghehu , Chekole Bogale , Gelaw Belete , Bayih Wubet Alebachew , Tesfaw Aragaw , Feleke Dejen Getaneh , Kefale Demewoz , Azmeraw Molla , Chanie Aynadis , Awoke Getaneh , Moges Natnael
Published: June 2023

Background

Globally, Pneumonia continues to be the leading cause of mortality among under-five children. Ethiopia ranks fourth out of 15 countries worldwide in terms of the highest death rate of under-five children due to severe community-acquired pneumonia (SCAP). However, to date, there is no recent study that shows survival status and predictors of mortality from SCAP. Therefore, this study aimed to determine survival status and predictors of mortality from this dangerous disease among under-five children.

Methods

A facility-based prospective cohort study was conducted from 1 November 2021 to 31 October 2022 at Debre Tabor comprehensive specialized hospital. All under-five children with SCAP admitted during the study period were included. Participants were selected using a systematic sampling technique. The collected data were coded, edited, and entered into epi-data version 4.2 and then exported to STATA version 17 for further analysis. The Kaplan Meier failure estimate with log-rank test was employed to determine the survival estimates. A cox-proportional hazard regression model was fitted to identify significant variables.

Results

The overall incidence density rate of mortality was 5.7 /1000 children with a median hospital stay of 8.2 days. Heart disease (AHR: 4.37; 95%CI: 1.68–11.32), previous admission of SCAP (AHR: 3.87; 95% CI: 1.31–11.43), WFL < −3Z score (AHR: 3.57; 95% CI: 1.02–12.42), impaired consciousness level at admission 3.41(1.14–10.19), and pleural effusion (AHR: 3.42; 95%CI: 1.18–9.93) were significant predictors of mortality.

Conclusion

In this study, the survival probability of children with SCAP was low. Children with heart disease, previous admission of SCAP, WFL < −3Z score, impaired consciousness level at admission, and pleural effusion had low survival. Therefore, much emphasis is needed on children with SCAP, particularly those with identified predictors.
A retrospective analysis of factors associated with the length of hospital stay in COVID-19 patients treated with Nirmatrelvir / Ritonavir
Authors: Zheng Jiantao , Hong Wencong , Zhou Chanjuan , Hong Donghuang , Yan Hong , Shen Yanghui
Published: June 2023

Objectives: This study reviewed factors influencing the length of hospital stay in adult inpatients with confirmed Coronavirus disease (COVID-19) who were treated with Nirmatrelvir/Ritonavir.

Methods: We did a retrospective analysis of data from a cohort of inpatients with confirmed diagnosis of Omicron variant of SARS-CoV-2 infection who were treated with Nirmatrelvir/Ritonavir. We included patients who were treated from 13th March 2022 to 6th May 2022 in various in-patient treatment units in Quanzhou, Fujian Province, China. The primary study outcome was the length of hospital stay. Secondary study outcome was viral elimination defined as negative for ORF1ab and N genes [cycle threshold (Ct) value ≥35 in real-time PCR], according to local guidelines. Hazard ratios (HR) of event outcomes were analyzed using Multivariate Cox regression models.

Results: We studied 31 inpatients with high risk for severe COVID-19 who were treated with Nirmatrelvir/Ritonavir. We found that inpatients with shorter length of hospital stay (≤17 days) were mostly females with lower body mass index (BMI) and Charlson Comorbidity Index (CCI) index. Their treatment regimen with Nirmatrelvir/Ritonavir was started within 5 days of diagnosis (p < 0.05). Multivariate Cox regression indicated that inpatients starting treatment of Nirmatrelvir/Ritonavir within 5 days had a shorter length of hospital stay (HR 3.573, p = 0.004) and had a faster clearance of viral load (HR 2.755, p = 0.043).

Conclusion: This study assumes relevance during the Omicron BA.2 epidemic as our findings suggest that early treatment with Nirmatrelvir/Ritonavir within 5 days of diagnosis (≤5 days) was highly effective in shortening the length of hospital stay and faster viral load clearance.
Cost-effectiveness of adding empagliflozin to the standard of care for patients with heart failure with reduced ejection fraction from the perspective of healthcare system in Malaysia
Authors: Ong Siew Chin , Low Joo Zheng , Linden Stephan
Published: June 2023

Objective: The aim of this study was to determine the cost-effectiveness of adding empagliflozin to the standard of care versus SoC alone for the treatment of patients with heart failure (HF) with reduced ejection fraction (HFrEF) from the perspective of the Ministry of Health of Malaysia.

Methods: A cohort-based transition-state model, with health states defined as Kansas City Cardiomyopathy Questionnaire Clinical Summary Score (KCCQ-CSS) quartiles and death, was used to determine the lifetime direct medical costs and quality-adjusted life years (QALYs) for both treatment groups. The risks of all-cause death, cardiovascular death, and health state utilities were estimated from the EMPEROR-Reduced trial. The incremental cost-effectiveness ratio (ICER) was assessed against the cost-effectiveness threshold (CET) as defined by the country’s gross domestic product per capita (RM 47,439 per QALY) to determine cost-effectiveness. Sensitivity analyses were conducted to assess the key model parameters’ uncertainty in respect to the incremental cost-effectiveness ratio. A scenario analysis was performed using health states as defined by the New York Heart Association classes.

Results: Compared to SoC alone, empagliflozin + SoC for the treatment of HFrEF was more expensive (RM 25,333 vs. RM 21,675) but gained more health utilities (3.64 vs. 3.46), resulting in an ICER of RM 20,400 per QALY in the KCCQ-CSS model. A NYHA-based scenario analysis generated an ICER of RM 36,682 per QALY. A deterministic sensitivity analysis confirmed the robustness of the model in identifying the empagliflozin cost as the main driver of cost-effectiveness. The ICER was reduced to RM 6,621 when the government medication purchasing prices were used. A probabilistic sensitivity analysis with a CET of 1xGDP per capita reached 72.9% probability for empagliflozin + SoC against SoC being cost-effective.

Conclusion: Empagliflozin + SoC compared to SoC alone for the treatment of HFrEF patients was cost-effective from the perspective of the MoH of Malaysia.
Dynamic interplay of WRKY, GRAS, and ERF transcription factor families in tomato-endophytic fungal symbiosis: insights from transcriptome and genome-wide analysis
Authors: Khan Ibrahim , Lubna  , Asaf Sajjad , Jan Rahmatullah , Bilal Saqib , Khan Abdul Latif , Kim Kyung-Min , Al-Harrasi Ahmed
Published: June 2023

Plant-microbe interactions play a crucial role in shaping plant growth and development, as well as in mediating plant responses to biotic and abiotic stresses. In this study, we used RNA-seq data to examine the expression profiles of SlWRKY, SlGRAS, and SlERF genes during the symbiotic association of Curvularia lunata SL1 with tomato (Solanum lycopersicum) plants. We also conducted functional annotation analysis by comparative genomics studies of their paralogs and orthologs genes, as well as other approaches, such as gene analysis and protein interaction networks, to identify and characterize the regulatory roles of these TFs in the development of the symbiotic association. We found that more than half of the investigated SlWRKY genes exhibited significant upregulation during symbiotic association, including SlWRKY38, SlWRKY46, SlWRKY19, and SlWRKY51. Several SlGRAS and SlERF genes were upregulated, such as SlGLD2, SlGLD1, SlERF.C.5, ERF16, and SlERF.B12. Conversely, a smaller proportion of SlWRKY, SlGRAS, and SlERF genes were significantly downregulated during symbiotic association. Furthermore, we investigated the possible roles of SlWRKY, SlGRAS, and SlERF genes in hormonal regulation during plant-microbe interactions. We identified several upregulated candidate transcripts likely to be involved in plant hormone signaling pathways. Our findings are consistent with previous studies on these genes, providing further evidence of their involvement in hormonal regulation during plant-microbe interactions. To validate the RNA-seq data accuracy, we performed RT-qPCR analyses of selected SlWRKY, SlGRAS, and SlERF genes, which showed similar expression patterns to those observed in the RNA-seq data. These results confirmed the accuracy of our RNA-seq data and provided additional support for the differential expression of these genes during plant-microbe interactions. Taken together, our study provides new insights into the differential expression profiles of SlWRKY, SlGRAS, and SlERF genes during symbiotic association with C. lunata, as well as their potential roles in hormonal regulation during plant-microbe interactions. These findings could be useful for guiding future research on the ways in which plants and microbes interact, and may ultimately lead to the creation of better approaches for promoting plant growth under stressful conditions.
QTL mapping and improvement of pre-harvest sprouting resistance using japonica weedy rice
Authors: Lee Chang-Min , Park Hyun-Su , Baek Man-Kee , Jeong O-Young , Seo Jeonghwan , Kim Suk-Man
Published: June 2023

The stability of cultivation and production in terms of crop yield has been threatened by climate change due to global warming. Pre-harvest sprouting (PHS) is a threat to crops, especially staple foods, including rice, because of reductions in yield and quality. To address the problem of precocious germination before harvest, we performed quantitative trait loci (QTL) analysis for PHS using F8 RILs populations derived from japonica weedy rice in Korea. QTL analysis revealed that two stable QTLs, qPH7 and qPH2, associated with PHS resistance were identified on chromosomes 7 and 2, respectively, explaining approximately 38% of the phenotypic variation. The QTL effect in the tested lines significantly decreased the degree of PHS, based on the number of QTLs included. Through fine mapping for main QTL qPH7, the region for the PHS was found to be anchored within 23.575–23.785 Mbp on chromosome 7 using 13 cleaved amplified sequence (CAPS) markers. Among 15 open reading frames (ORFs) within the detected region, one ORF, Os07g0584366, exhibited upregulated expression in the resistant donor, which was approximately nine times higher than that of susceptible japonica cultivars under PHS-inducing conditions. Japonica lines with QTLs related to PHS resistance were developed to improve the characteristics of PHS and design practical PCR-based DNA markers for marker-assisted backcrosses of many other PHS-susceptible japonica cultivars.
Fungal strain and crop cultivar affect growth of sweet pepper plants after root inoculation with entomopathogenic fungi
Authors: Wilberts Liesbet , Rojas-Preciado Nicolas , Jacquemyn Hans , Lievens Bart
Published: June 2023

As endophytes, entomopathogenic fungi can protect plants against biotic and abiotic stresses and at the same time promote plant growth and plant health. To date, most studies have investigated whether Beauveria bassiana can enhance plant growth and plant health, while only little is known about other entomopathogenic fungi. In this study, we evaluated whether root inoculation of the entomopathogenic fungi Akanthomyces muscarius ARSEF 5128, B. bassiana ARSEF 3097 and Cordyceps fumosorosea ARSEF 3682 can promote plant growth of sweet pepper (Capsicum annuum L.), and whether effects are cultivar-dependent. Plant height, stem diameter, number of leaves, canopy area, and plant weight were assessed four weeks following inoculation in two independent experiments using two cultivars of sweet pepper (cv. ‘IDS RZ F1’ and cv. ‘Maduro’). Results showed that the three entomopathogenic fungi were able to enhance plant growth, particularly canopy area and plant weight. Further, results showed that effects significantly depended on cultivar and fungal strain, with the strongest fungal effects obtained for cv. ‘IDS RZ F1’, especially when inoculated with C. fumosorosea. We conclude that inoculation of sweet pepper roots with entomopathogenic fungi can stimulate plant growth, but effects depend on fungal strain and crop cultivar.
Relationship between family background and self-efficacy in adolescent table tennis players: a moderated mediation model
Authors: He Ke , Li Weiming , Li Zihao
Published: June 2023

Introduction

A moderated mediation model was constructed in this study to clarify the relationship between family background and self-efficacy of adolescent table tennis players, focusing on the mediating effect of technical learning engagement in the relationship as well as the moderating role of factors such as gender and training years.

Methods

189 adolescent table tennis players (age: 13.69±1.28 years) were investigated as subjects using a questionnaire method.

Results

(1) Family background, technical learning engagement, and self-efficacy were significantly and positively correlated (p<0.01), with girls’ technical learning engagement (Mfemale=5.81, Mmale=5.19, p<0.01) and self-efficacy (Mfemale=3.34, Mmale=2.66, p<0.01) significantly higher than boys’; (2) Technical learning engagement partially mediated the effect of family background on self-efficacy (ab=0.10, boot SE=0.02,95% CI=[0.07, 0.14]); (3) The first half of technical learning engagement’s mediating role was moderated by gender (B=0.05, p<0.01), with a more significant influence of family background on boys’ (B=0.24, p<0.001, 95% CI=[0.22, 0.26]) technical learning engagement than girls’ (B=0.19, p<0.001, 95% CI=[0.17, 0.21]); (4) The second half of technical learning engagement’s mediating role was moderated by training years (B=–0.21, p<0.001), with a more significant influence of technical learning engagement on the self-efficacy of adolescents with fewer training years (B=0.54, p<0.001, 95% CI=[0.39, 0.68]). The positive effect of technical learning engagement on self-efficacy gradually diminished with increasing training years, and the moderating effect of training years disappeared when the training years reached 8.94 years.

Conclusion

(1) More attention should be paid to adolescent table tennis players with poor family backgrounds, who are more likely to have low self-efficacy. (2) Parents should never neglect their initiative in providing guidance and support to adolescent players involved in long-term professional table tennis training, especially for boys. (3) Coaches should pay close attention to the level of technical learning engagement of players with long training years, who are more likely to have lower self-efficacy as a result of their own emotional experiences, stagnant performance, etc.
Speakers are more cooperative and less individual when interacting in larger group sizes
Authors: Pellegrino Elisa , Dellwo Volker
Published: June 2023

Introduction

Cooperation, acoustically signaled through vocal convergence, is facilitated when group members are more similar. Excessive vocal convergence may, however, weaken individual recognizability. This study aimed to explore whether constraints to convergence can arise in circumstances where interlocutors need to enhance their vocal individuality. Therefore, we tested the effects of group size (3 and 5 interactants) on vocal convergence and individualization in a social communication scenario in which individual recognition by voice is at stake.

Methods

In an interactive game, players had to recognize each other through their voices while solving a cooperative task online. The vocal similarity was quantified through similarities in speaker i-vectors obtained through probabilistic linear discriminant analysis (PLDA). Speaker recognition performance was measured through the system Equal Error Rate (EER).

Results

Vocal similarity between-speakers increased with a larger group size which indicates a higher cooperative vocal behavior. At the same time, there was an increase in EER for the same speakers between the smaller and the larger group size, meaning a decrease in overall recognition performance.

Discussion

The decrease in vocal individualization in the larger group size suggests that ingroup cooperation and social cohesion conveyed through acoustic convergence have priority over individualization in larger groups of unacquainted speakers.
Psychological restoration depends on curiosity, motivation, and species richness during a guided bird walk in a suburban blue space
Authors: Randler Christoph , Vanhöfen Janina , Härtel Talia , Neunhoeffer Freya , Engeser Cheyenne , Fischer Christian
Published: June 2023

Urban and suburban green and blue spaces are important places for human recreation, and the impact of biodiversity on psychological and recalled restoration has received much attention. This study addresses the relationship between bird species richness and restoration in a controlled field experiment (guided bird walk) applying a battery of individual trait scales (need for cognition, personality) as predictors of restoration. We found a significant positive relationship between the number of bird species present and recalled restoration. Personality, bird species knowledge, bird related interest as test measures, demographics and birding specialization as self-report had no influence on psychological restoration. However, need for cognition correlated positively with psychological restoration, thus providing a new predictive variable. All subscales of the intrinsic motivation scale (enjoyment, perceived competence, perceived choice, pressure/tension) were positively correlated with restoration except of pressure/tension. Learning emotions like interest and well-being were positively related to restoration, while boredom was negatively related. Therefore, we suggest research to examine the restorative function of more cognitive-oriented programs because people may also need cognition when it comes to restoration. We also suggest a broader focus on education and cognitive aspects when it comes to linking biodiversity and health within the framework of ecosystem services.
Stop it! Relationship between sport expertise and response inhibition in elite athletes
Authors: Fleddermann Marie-Therese , Reichert Lukas , Wieland Björn , Zentgraf Karen
Published: June 2023

Introduction

The dynamic structure of sport games forces players to make time-sensitive decisions and to initiate actions that may then have to be canceled in response to sudden changes in the game situation. Whether and up to which time already initiated movements can still be inhibited is an important criterion for game performance in elite sport. Research indicates that elite athletes show superior motor inhibition performance compared to recreational athletes. However, no study has examined whether differences also emerge among professional elite athletes themselves. Therefore, this study aimed to investigate whether motor inhibition performance is a differential feature among elite athletes, and whether inhibition performance increases with greater expertise.

Methods

In total of 106 elite athletes (ice hockey, basketball, volleyball, American football, handball, and soccer) completed a PC-based procedure to determine motor inhibition performance using the stop-signal reaction time (SSRT) task for hands and feet. In addition, an expertise score was determined for each elite athlete. Multiple linear regression was used to calculate the relationship between expertise and SSRT.

Results

Results showed that the expertise score of the elite athletes was between 3.7 and 11.7 out of 16 possible points (MExpertise = 6.8 points, SD = 1.76). The average SSRT of the hands was 224.0 ms (SD = 35.0); of the feet, 257.9 ms (SD = 48.5). Regression results showed a significant relationship between expertise and SSRT (F(2,101) = 9.38, p = 0.04, R2 = 0.06). SSRTs of the hands were significant predictors of expertise (b = −0.23, t = −2.1, p = 0.04).

Discussion

Taken together, results suggest that elite athletes with higher expertise outperform elite athletes with lower expertise, indicating that it is possible to differentiate within elite athletes with respect to inhibition performance of the hands. However, whether expertise affects inhibition performance or vice versa cannot be answered at present.
Self-referral practice and associated factors among women who gave birth in South Gondar zone primary hospitals, Northwest Ethiopia: a cross-sectional study design
Authors: Eshetie Ayenew , Belachew Tadele Biresaw , Negash Wubshet Debebe , Asmamaw Desale Bihonegn , Muktar Sualiha Abdulkader , Kebede Adane
Published: June 2023

Background

Patient self-referral is when patients refer themselves to upper-level health facilities without having to see anyone else first or without being told to refer themselves by another health professional. Self-referral leads to a diminished quality of healthcare services. However, globally, many women who gave birth referred themselves to hospitals without having referral sheets, including in Ethiopia and the study area. Therefore, this study aimed to assess self-referral practice and associated factors among women who gave birth in South Gondar zone primary hospitals in Northwest Ethiopia.

Methods

A cross-sectional mixed-method study was conducted among women who gave birth in South Gondar zone primary hospitals between 1 June 2022 and 15 July 2022. Semi-structured questionnaires were used to gather quantitative data from 561 participants who were selected by a systematic random sampling technique. Interview guides were used to collect qualitative data from selected six key informants. Quantitative data were entered into Epi Data version 4.6.0.4 and then exported to the statistical software SPSS version 25 for further analysis. Thematic analysis using open code version 4.02 software was applied for qualitative data analysis. A binary logistic regression analysis was employed. In a bivariable analysis, a p < 0.25 was used to select candidate variables for multivariable analysis. P < 0.05 and a 95% confidence interval were used to determine significant variables on the outcome of interest.

Results

The overall magnitude of self-referral was 45.6%, with 95% CI (41.5%, 49.9%). They had no antenatal care (ANC) follow-up (AOR = 3.02, 95% CI: 1.64–5.57) and 1–3 ANC follow-ups (AOR = 1.57, 95% CI: 1.03–2.41), poor knowledge about the referral system (AOR = 4.04, 95% CI: 2.30–7.09), and use of public transportation (AOR = 2.34, 95% CI: 1.43–3.82), which were significantly associated with self-referral practice.

Conclusion

This study showed that nearly half of the deliveries were self-referred. ANC follow-up, women's knowledge of the referral system, and mode of transportation were factors significantly associated with the self-referral practice. Therefore, developing awareness-creation strategies and increasing coverage of ANC 4 and above are necessary interventions to reduce the self-referral practice.
Auxiliary screening COVID-19 by computed tomography
Authors: Pan Xiongfeng , Chen Yuyao , Kaminga Atipatsa C. , Wen Shi Wu , Liu Hongying , Jia Peng , Liu Aizhong
Published: June 2023

Background

The 2019 novel coronavirus (COVID-19) pandemic remains rampant in many countries/regions. Improving the positive detection rate of COVID-19 infection is an important measure for the control and prevention of this pandemic. This meta-analysis aims to systematically summarize the current characteristics of the computed tomography (CT) auxiliary screening methods for COVID-19 infection in the real world.

Methods

Web of Science, Cochrane Library, Embase, PubMed, CNKI, and Wanfang databases were searched for relevant articles published prior to 1 September 2022. Data on specificity, sensitivity, positive/negative likelihood ratio, area under curve (AUC), and diagnostic odds ratio (dOR) were calculated purposefully.

Results

One hundred and fifteen studies were included with 51,500 participants in the meta-analysis. Among these studies, the pooled estimates for AUC of CT in confirmed cases, and CT in suspected cases to predict COVID-19 diagnosis were 0.76 and 0.85, respectively. The CT in confirmed cases dOR was 5.51 (95% CI: 3.78–8.02). The CT in suspected cases dOR was 13.12 (95% CI: 11.07–15.55).

Conclusion

Our findings support that CT detection may be the main auxiliary screening method for COVID-19 infection in the real world.
“It's a lot more complicated than it seems”: physiotherapists' experiences of using compensation strategies in people with Parkinson's
Authors: Alenezi Sheemah , Morgan-Trimmer Sarah , Hulbert Sophia , Young William , Goodwin Victoria A.
Published: June 2023

Background

Gait disturbances often result in functional limitations in daily activities and negatively impact the quality of life in people with Parkinson's disease. Physiotherapists often employ compensation strategies in an attempt to improve patients' walking. However, little is known about physiotherapists' experiences in this regard. We evaluated how physiotherapists adopt compensation strategies and what they draw on to inform their clinical decision-making.

Methods

We carried out semi-structured online interviews with 13 physiotherapists with current or recent experience working with people with Parkinson's disease in the United Kingdom. Interviews were digitally recorded and transcribed verbatim. Thematic analysis was utilized.

Results

Two main themes were developed from the data. The first theme, optimizing compensation strategies through personalized care, shows how physiotherapists accounted for the individual needs and characteristics of people with Parkinson's, which resulted in them individually tailoring compensation strategies. The second theme, delivering compensation strategies effectively, considers the available support and perceived challenges with work settings and experience that impact physiotherapists' ability to deliver compensation strategies.

Discussion

Although physiotherapists strived to optimize compensation strategies, there was a lack of formal training in this area, and their knowledge was primarily acquired from peers. Furthermore, a lack of specific knowledge on Parkinson's can impact physiotherapists’ confidence in maintaining person-centered rehabilitation. However, the question that remains to be answered is what accessible training could address the knowledge–practice gap to contribute to the delivery of better-personalized care for people with Parkinson's.
Household production and consumption impacts of foot and mouth disease at the Uganda-Tanzania border
Authors: Kerfua Susan Diana , Railey Ashley Flynn , Marsh Thomas Lloyd
Published: June 2023

Introduction

Foot-and–mouth disease (FMD) is a highly contagious viral disease that is endemic in East Africa. FMD virus infection incurs significant control costs and reduces animal productivity through weight loss, lowered milk yield, and potentially death but how household’s respond to these losses may differentially affect household income and food consumption.

Methodology

To address this, we use unique data from a FMD outbreak to assess how household production and consumption activities change from before to during the outbreak. Data came from a 2018 survey of 254 households in selected Tanzanian wards and sub-counties in Uganda. The data includes household recall of before and during an outbreak in the past year on livestock and livestock product sales, milk and beef consumption, as well as related changes in market prices. We apply both difference-in-difference and change in difference ordinary least squares regressions with fixed effects to evaluate the impact of FMD on household production and consumption.

Results and discussion

We find that households reported the largest reductions in livestock and livestock product sales, followed by reduced milk consumption and animal market prices. The changes in household income from livestock sales appears to be driven by FMD virus infection within the household herd while changes in market prices of substitute protein sources are primary associated with changes in milk and beef consumption. The role of widespread market price effects across both infected and uninfected herds and countries, tends to suggest that stabilizing prices will likely have a large impact on household nutritional security and income generation. We also propose that promoting diversity in market activity may mitigate differing impacts on households in FMD endemic regions.