bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiparametric assessment of mitochondrial respiratory inhibition in HepG2 and RPTEC/TERT1 cells using a panel of mitochondrial targeting agrochemicals
Authors: van der Stel Wanda , Carta Giada , Eakins Julie , Darici Salihanur , Delp Johannes , Forsby Anna , Bennekou Susanne Hougaard , Gardner Iain , Leist Marcel , Danen Erik H. J. , Walker Paul , van de Water Bob , Jennings Paul
Published: June 2020

Evidence is mounting for the central role of mitochondrial dysfunction in several pathologies including metabolic diseases, accelerated ageing, neurodegenerative diseases and in certain xenobiotic-induced organ toxicity. Assessing mitochondrial perturbations is not trivial and the outcomes of such investigations are dependent on the cell types used and assays employed. Here we systematically investigated the effect of electron transport chain (ETC) inhibitors on multiple mitochondrial-related parameters in two human cell types, HepG2 and RPTEC/TERT1. Cells were exposed to a broad range of concentrations of 20 ETC-inhibiting agrochemicals and capsaicin, consisting of inhibitors of NADH dehydrogenase (Complex I, CI), succinate dehydrogenase (Complex II, CII) and cytochrome bc1 complex (Complex III, CIII). A battery of tests was utilised, including viability assays, lactate production, mitochondrial membrane potential (MMP) and the Seahorse bioanalyser, which simultaneously measures extracellular acidification rate [ECAR] and oxygen consumption rate [OCR]. CI inhibitors caused a potent decrease in OCR, decreased mitochondrial membrane potential, increased ECAR and increased lactate production in both cell types. Twenty-fourhour exposure to CI inhibitors decreased viability of RPTEC/TERT1 cells and 3D spheroid-cultured HepG2 cells in the presence of glucose. CI inhibitors decreased 2D HepG2 viability only in the absence of glucose. CII inhibitors had no notable effects in intact cells up to 10 µM. CIII inhibitors had similar effects to the CI inhibitors. Antimycin A was the most potent CIII inhibitor, with activity in the nanomolar range. The proposed CIII inhibitor cyazofamid demonstrated a mitochondrial uncoupling signal in both cell types. The study presents a comprehensive example of a mitochondrial assessment workflow and establishes measurable key events of ETC inhibition.
On the Average Case of MergeInsertion
Authors: Stober Florian , Weiß Armin
Published: June 2020

MergeInsertion, also known as the Ford-Johnson algorithm, is a sorting algorithm which, up to today, for many input sizes achieves the best known upper bound on the number of comparisons. Indeed, it gets extremely close to the information-theoretic lower bound. While the worst-case behavior is well understood, only little is known about the average case. This work takes a closer look at the average case behavior. In particular, we establish an upper bound of $n \log n - 1.4005n + o(n)$
n
log
n
−
1.4005
n
+
o
(
n
)
comparisons. We also give an exact description of the probability distribution of the length of the chain a given element is inserted into and use it to approximate the average number of comparisons numerically. Moreover, we compute the exact average number of comparisons for n up to 148. Furthermore, we experimentally explore the impact of different decision trees for binary insertion. To conclude, we conduct experiments showing that a slightly different insertion order leads to a better average case and we compare the algorithm to Manacher’s combination of merging and MergeInsertion as well as to the recent combined algorithm with (1,2)-Insertionsort by Iwama and Teruyama.
Impact of nutritional index on long-term outcomes of elderly patients with coronary artery disease: sub-analysis of the SHINANO 5 year registry
Authors: Maruyama Shusaku , Ebisawa Souichiro , Miura Takashi , Yui Hisanori , Kashiwagi Daisuke , Nagae Ayumu , Sakai Takahiro , Kato Tamon , Saigusa Tatsuya , Okada Ayako , Motoki Hirohiko , Kuwahara Koichiro
Published: June 2020

Nutritional status is a novel approach to prognostic assessment in patients with cardiovascular disease. However, assessment of nutritional status in elderly patients is challenging due to the significant differences between young patients. The TCBI (Triglycerides × Total cholesterol × Body Weight Index) is a novel and simple nutritional index for predicting long-term outcomes in patients with coronary artery disease. This retrospective study evaluated the efficacy of TCBI in 597 elderly (≥ 75 years) patients enrolled in the SHINANO 5 year registry. The SHINANO 5 year registry, a prospective observational multicenter cohort study, had enrolled 1501 consecutive patients who underwent elective/urgent percutaneous coronary intervention (PCI). In this study, patients were categorized into TCBI quartile groups. The primary endpoints were the occurrence of major adverse cardiac and cerebrovascular events (MACCE), including all-cause death, stroke, and myocardial infarction at 5 year. The mean duration of follow up was 4.3 ± 1.7 years. The average patient age was 80.9 ± 4.3 years. MACCE was observed in 61 (40.9%) patients in the lowest TCBI quartile group. Kaplan–Meier analysis demonstrated an inverse relationship between MACCE and TCBI (log-lank P < 0.001). Multivariate analysis demonstrated that low TCBI significantly predicted the incidence of MACCE (hazard ratio: 1.44, 95% confidence interval: 1.03–2.00; P = 0.031). The TCBI is useful in predicting long-term outcomes in elderly patients undergoing PCI.
Sirtuin1 expression and survival in endometrial and clear-cell uterine cancer
Authors: Beyer Susanne , Chen Fangfang , Meister Sarah , Czogalla Bastian , Kolben Theresa M. , Hester Anna , Burges Alexander , Trillsch Fabian , Schmöckel Elisa , Mayr Doris , Mayerhofer Artur , Mahner Sven , Jeschke Udo , Kolben Thomas
Published: June 2020

Several risk factors like obesity and hyperlipidemia were described for endometrial cancer. Here, the nuclear NAD-dependent histone-deacetylase Sirtuin1 (SIRT1) seems to be important. SIRT1 is also involved in cell regulatory mechanisms and can serve as tumor promotor or suppressor. Its role in tumor biology is not clear yet. In this study, we evaluated and correlated the SIRT1 expression with patients’ tumor characteristics in endometrioid and clear-cell cancer of the uterus. 65 paraffin-embedded samples of patients with endometrial and clear-cell cancer of the uterus were immunohistochemically stained and SIRT1 expression was evaluated by immunoreactive score. The results were correlated to clinical and pathological tumor characteristics as well as to the expression of ARID1A and β-Catenin. The staining was significantly more intensive in uterine endometrioid carcinoma compared to uterine clear-cell carcinoma (p = 0.007). The expression of SIRT1 correlated significantly with the membranous expression of β-Catenin (p = 0.028) and ARID1A (p = 0.021). Patients with positive Sirtuin1 expression had a significantly better progression-free survival (p = 0.042), the overall survival showed a trend towards a better prognosis (p = 0.070). SIRT1 expression seems to be associated with improved progression-free survival in uterine cancer (endometrioid and clear-cell) and is correlated to the tumor suppressors β-Catenin and ARID1A. Further studies are necessary to elucidate the role of SIRT1 in uterine and ovarian cancer and its potential as a therapeutic target.
Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish
Authors: Bosze Bernadett , Ono Yosuke , Mattes Benjamin , Sinner Claude , Gourain Victor , Thumberger Thomas , Tlili Sham , Wittbrodt Joachim , Saunders Timothy E. , Strähle Uwe , Schug Alexander , Scholpp Steffen
Published: June 2020

The notochord defines the axial structure of all vertebrates during development. Notogenesis is a result of major cell reorganization in the mesoderm, the convergence and the extension of the axial cells. However, it is currently not fully understood how these processes act together in a coordinated way during notochord formation. The prechordal plate is an actively migrating cell population in the central mesoderm anterior to the trailing notochordal plate cells. We show that prechordal plate cells express Protocadherin 18a (Pcdh18a), a member of the cadherin superfamily. We find that Pcdh18a-mediated recycling of E-cadherin adhesion complexes transforms prechordal plate cells into a cohesive and fast migrating cell group. In turn, the prechordal plate cells subsequently instruct the trailing mesoderm. We simulated cell migration during early mesoderm formation using a lattice-based mathematical framework and predicted that the requirement for an anterior, local motile cell cluster could guide the intercalation and extension of the posterior, axial cells. Indeed, a grafting experiment validated the prediction and local Pcdh18a expression induced an ectopic prechordal plate-like cell group migrating towards the animal pole. Our findings indicate that the Pcdh18a is important for prechordal plate formation, which influences the trailing mesodermal cell sheet by orchestrating the morphogenesis of the notochord.
Solving visual object ambiguities when pointing: an unsupervised learning approach
Authors: Jirak Doreen , Biertimpel David , Kerzel Matthias , Wermter Stefan
Published: June 2020

Whenever we are addressing a specific object or refer to a certain spatial location, we are using referential or deictic gestures usually accompanied by some verbal description. Particularly, pointing gestures are necessary to dissolve ambiguities in a scene and they are of crucial importance when verbal communication may fail due to environmental conditions or when two persons simply do not speak the same language. With the currently increasing advances of humanoid robots and their future integration in domestic domains, the development of gesture interfaces complementing human–robot interaction scenarios is of substantial interest. The implementation of an intuitive gesture scenario is still challenging because both the pointing intention and the corresponding object have to be correctly recognized in real time. The demand increases when considering pointing gestures in a cluttered environment, as is the case in households. Also, humans perform pointing in many different ways and those variations have to be captured. Research in this field often proposes a set of geometrical computations which do not scale well with the number of gestures and objects and use specific markers or a predefined set of pointing directions. In this paper, we propose an unsupervised learning approach to model the distribution of pointing gestures using a growing-when-required (GWR) network. We introduce an interaction scenario with a humanoid robot and define the so-called ambiguity classes. Our implementation for the hand and object detection is independent of any markers or skeleton models; thus, it can be easily reproduced. Our evaluation comparing a baseline computer vision approach with our GWR model shows that the pointing-object association is well learned even in cases of ambiguities resulting from close object proximity.
Single vs. multi-cone synchronizers with carbon friction lining—a comparison of load limits and deterioration behavior
Authors: Stockinger U. , Schneider T. , Pflaum H. , Stahl K.
Published: June 2020

Synchronizers are widely used in gear boxes of car and truck transmissions. Optimizing costs and improving the efficiency of gear boxes require knowledge about the relative load limits of single and multi-cone synchronizers with carbon friction linings and their corresponding deterioration mechanisms. Load limits for single and multi-cone synchronizers with carbon friction lining used in cars were determined experimentally using the component test rig SSP-180 and compared against each other. Different load stages were run in life cycle tests and the influences of pressure, sliding velocity, friction work, frictional power and oil temperature were investigated. The synchronizers’ failure mode is described and the novel specific value μmin,grad is introduced that is able to quantify the deterioration of different synchronizer systems. During life cycle tests at the same specific load level, the single cone synchronizers, contrary to expectations, displayed greater damage compared to the multi-cone synchronizers. Calculations of the friction surface temperature in thermo-mechanical simulations, in combination with experiments on the test rig, show that the maximum temperature during the engagement has a significant influence on deterioration and endurance life of synchronizers with carbon friction lining.
Implementing a micromechanical model into a finite element code to simulate the mechanical and microstructural response of arteries
Authors: Bianchi Daniele , Morin Claire , Badel Pierre
Published: June 2020

A computational strategy based on the finite element method for simulating the mechanical response of arterial tissues is herein proposed. The adopted constitutive formulation accounts for rotations of the adventitial collagen fibers and introduces parameters which are directly measurable or well established. Moreover, the refined constitutive model is readily utilized in finite element analyses, enabling the simulation of mechanical tests to reveal the influence of microstructural and histological features on macroscopic material behavior. Employing constitutive parameters supported by histological examinations, the results herein validate the model’s ability to predict the micro- and macroscopic mechanical behavior, closely matching previously observed experimental findings. Finally, the capabilities of the adopted constitutive description are shown investigating the influence of some collagen disorders on the macroscopic mechanical response of the arterial tissues.
A proposed unified framework to describe the management of biological invasions
Authors: Robertson Peter A. , Mill Aileen , Novoa Ana , Jeschke Jonathan M. , Essl Franz , Gallardo Belinda , Geist Juergen , Jarić Ivan , Lambin Xavier , Musseau Camille , Pergl Jan , Pyšek Petr , Rabitsch Wolfgang , von Schmalensee Menja , Shirley Mark , Strayer David L. , Stefansson Robert A. , Smith Kevin , Booy Olaf
Published: June 2020

Managing the impacts of invasive alien species (IAS) is a great societal challenge. A wide variety of terms have been used to describe the management of invasive alien species and the sequence in which they might be applied. This variety and lack of consistency creates uncertainty in the presentation and description of management in policy, science and practice. Here we expand on the existing description of the invasion process to develop an IAS management framework. We define the different forms of active management using a novel approach based on changes in species status, avoiding the need for stand-alone descriptions of management types, and provide a complete set of potential management activities. We propose a standardised set of management terminology as an emergent feature of this framework. We identified eight key forms of management: (1) pathway management, (2) interception, (3) limits to keeping, (4) secure keeping, (5) eradication, (6) complete reproductive removal, (7) containment and (8) suppression. We recognise four associated terms: prevention; captive management; rapid eradication; and long-term management, and note the use of impact mitigation and restoration as associated forms of management. We discuss the wider use of this framework and the supporting activities required to ensure management is well-targeted, cost-effective and makes best use of limited resources.
Detection, assessment and mitigation of vulnerabilities in open source dependencies
Authors: Ponta Serena Elisa , Plate Henrik , Sabetta Antonino
Published: June 2020

Open source software (OSS) libraries are widely used in the industry to speed up the development of software products. However, these libraries are subject to an ever-increasing number of vulnerabilities that are publicly disclosed. It is thus crucial for application developers to detect dependencies on vulnerable libraries in a timely manner, to precisely assess their impact, and to mitigate any potential risk. This paper presents a novel method to detect, assess and mitigate OSS vulnerabilities. Differently from state-of-the-art approaches that depend on metadata to identify vulnerable OSS dependencies, our solution is code-centric, and combines static and dynamic analyses to determine the reachability of the vulnerable portion of libraries, in the context of a given application. Our approach also supports developers in choosing among the existing non-vulnerable library versions, with the goal to determine and minimize incompatibilities. , the open source implementation of our code-centric and usage-based approach is the tool recommended to scan Java software products at SAP; it has been successfully used to perform more than one million scans of about 1500 applications. In this paper we report on the lessons learned when maturing the tool from a research prototype to an industrial-grade solution. To evaluate , we conducted an empirical study to compare its detection capabilities with those of OWASP Dependency Check (), scanning 300 large enterprise applications under development with a total of 78165 dependencies. Reviewing a sample of the findings reported only by one of the two tools revealed that all findings are true positives, while 88.8% of the findings of for vulnerabilities covered by our code-centric approach are false positives. For vulnerabilities not caused by code but due, e.g., to erroneous configuration, 63.3% of findings are true positives.
A trend of increasing burned areas in Iraq from 2001 to 2019
Authors: Rasul Azad , Ibrahim Gaylan R. Faqe , Hameed Hasan M. , Tansey Kevin
Published: June 2020

Wildfires have an important role in the ecological process affecting ecosystems at multiple spatial scales. MCD64A1 500 m burned area product is used in this study to document trends of fire events from 2001 to 2019. The research aims to analyze the trend of burned areas in the country. Iraq is selected as study area of the research because of dramatically increasing the number of fires in the country. The study found that the lowest amount of burned areas were located in the center and southwest, while northeast and north faced most of the fires. The trend of burned areas in Iraq was an increase of 71.7 km2 per year, and, principally, in the last years, burned areas increased by 1363 km2. The most extensive burning areas occurred in the Ninawa Governorate (29%). In 2019, burned areas increased to around eight times compared to the yearly average. During 2019, one of the highest classes which contain burned area is “Cropland, rainfed” with 4366 km2 burned. High maximum air temperature and wind speed are the main factors that contribute to increasing burned areas; however, high precipitation and air pressure lead to a reducing amount of burned areas. The rapid increase of fires in the country as a result of burn crop field residues will lead to worsening air pollution and likely decrease forest area in the future.
Impact of local- and regional-scale restoration measures on a vulnerable rich fen in the Naardermeer nature reserve (the Netherlands)
Authors: Veeken Annegreet , Wassen Martin J.
Published: June 2020

Restoration of rich fens is commonly attempted through local-scale measures, such as removal of sod or blockage of ditches. However, regional-scale restoration measures, that aim to re-establish the original hydrology in which rich fens developed, might have a more long-lasting effect. We investigated the effect of local- and regional-scale restoration measures on a vulnerable rich fen in the Naardermeer nature reserve in the Netherlands. We compared water quality and vegetation composition of the fen before and after the restoration measures, almost 30 years apart. Overall rich fen species increased and although this indicates the desired increased supply of fresh mineral-rich groundwater to the fen, continued succession towards poor fen vegetation has not been prevented in the entire fen. Despite sod layer removal, we observed an increase in a Polytrichum-dominated vegetation in patches that are primarily fed by rainwater. Our findings confirm results from a previous study which showed that brackish palaeo-groundwater is still contributing substantially to the water balance of the fen, especially in periods of precipitation deficit. We conclude that the local- and regional-scale restoration measures have been successful in increasing the abundance of rich fen species in parts of the fen. However, considering the pressures of climate change and high atmospheric N-deposition on the fen, it is uncertain whether rich fen species can be sustained in quite nutrient-poor conditions in the future. Therefore, there is a need for continued management that keeps the nutrient-poor and mineral-rich conditions of the fen intact.
How value co-creation and co-destruction unfolds: a longitudinal perspective on dialogic engagement in health services interactions
Authors: Keeling Debbie Isobel , Keeling Kathleen , de Ruyter Ko , Laing Angus
Published: June 2020

Complex services, such as healthcare, struggle to realize the benefits of value co-creation due to the substantial challenges of managing such services over the long-term. Key to overcoming these challenges to value co-creation is a profound understanding of dialogue (i.e., ‘quality of discourse’ facilitating shared meaning) during service interactions. Contributing to an emerging literature, we undertake a longitudinal, ethnographic study to assess dialogue between professionals and patients through the lens of dialogic engagement (i.e., iterative mutual learning processes that bring about action through dialogue). We develop and empirically support six dialogic co-creation and co-destruction mechanisms that impact on the resolution of tensions and integration of knowledge resources between service providers and consumers. We reveal the multidimensional and dynamic nature of value created or destroyed through these mechanisms in dialogue over time. Taking healthcare as an exemplar, we offer a research agenda for developing our understanding of DE in complex services.
Silencing of functional p53 attenuates NAFLD by promoting HMGB1-related autophagy induction
Authors: Zhang Xuequn , Lin Yiming , Lin Sisi , Li Chunxiao , Gao Jianguo , Feng Zemin , Wang Jinghua , Zhang Jie , Zhang Hong , Zhang Yuwei , Chen Xueyang , Chen Shenghui , Xu Chengfu , Li Youming , Yu Chaohui , Zeng Hang
Published: June 2020

Background and aim

Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease worldwide, but its pathogenesis remains imprecisely understood and requires further clarification. Recently, the tumor suppressor p53 has received growing attention for its role in metabolic diseases. In this study, we performed in vivo and in vitro experiments to identify the contribution of p53–autophagy regulation to NAFLD.

Methods

Livers from wild-type and p53 knockout mice as well as p53-functional HepG2 cells and p53-dysfunctional Huh7 cells were examined for autophagy status and HMGB1 translocation. In vivo and in vitro NAFLD models were established, and steatosis was detected. In the cell models, autophagy status and steatosis were examined by p53 and/or HMGB1 silencing.

Results

First, the silencing of p53 could induce autophagy both in vivo and in vitro. In addition, p53 knockout attenuated high-fat diet-induced NAFLD in mice. Similarly, knockdown of p53 could alleviate palmitate-induced lipid accumulation in cell models. Furthermore, high mobility group box 1 (HMGB1) was proven to contribute to the effect of silencing p53 on alleviating NAFLD in vitro as an autophagy regulator.

Conclusion

The anti-NAFLD effect of functional p53 silencing is associated with the HMGB1-mediated induction of autophagy.

Graphic abstract
ADMA mediates gastric cancer cell migration and invasion via Wnt/β-catenin signaling pathway
Authors: Guo Q. , Xu J. , Huang Z. , Yao Q. , Chen F. , Liu H. , Zhang Z. , Lin J.
Published: June 2020

Objective

To explore the role of ADMA in gastric cancer.

Methods

The specimens of 115 gastric cancer patients were analyzed by ELISA and survival analysis. Functional assays were used to assess the effects of ADMA on gastric cancer cells. Experiments were conducted to detect the signaling pathway induced by ADMA in GC.

Results

Gastric cancer patients with high ADMA levels had poor prognosis and low survival rate. Furthermore, high level of ADMA did not affect the proliferation while promoted the migration and invasion of gastric cancer cell. Moreover, ADMA enhanced the epithelial–mesenchymal transition (EMT). Importantly, ADMA positively regulated β-catenin expression in GC and promoted GC migration and invasion via Wnt/β-catenin pathway.

Conclusions

ADMA regulates gastric cancer cell migration and invasion via Wnt/β-catenin signaling pathway and which may be applied to clinical practice as a diagnostic and prognostic biomarker.
Relationship between landslide and morpho-structural analysis: a case study in Northeast of Morocco
Authors: Benzougagh Brahim , Meshram Sarita Gajbhiye , Baamar Brahim , Dridri Abdallah , Boudad Larbi , Sadkaoui Driss , Mimich Khalid
Published: June 2020

The study of landslides in the Inaouene watershed (northeastern Morocco) provides information on the relationships between landslides and morpho-structural analysis. Landslides affect part of the slopes and slopes of the valley. The configuration of this relationship is controlled by the combination of several predisposing factors. Two localities were studied, located in similar morpho-structural contexts, but characterized by a different lithology. The first determining factor was lithology, in particular the dominance of friable geological formations, especially marls in the locality of Chebabate and limestone soft formations in the locality of Tahla. The second factor was the tectonics, which is well individualized in the limestone of Tahla; in spite of friable lithology of the marls of Chebabate, one manages to disturb traces of the tectonics notably with the level of intercalation of the sandstone benches. Another factor that controls the dissection and the evolution of the landslides is the precipitation by the effect of abundance of the water of impregnation in the formations. The fieldwork is devoted to the measurement of the different faults encountered in the two localities to make the stereographic projection in order to elaborate canvas. The principal objective of this study is to find the relation between the evolution of the risks of erosion and landslide as well as the various factors, who control their spatiotemporal evolution. The results obtained will help managers and decision-makers in the development of watersheds in order to take the necessary steps to reduce the negative impact of this natural hazard on the environment, the population and their property.
Potential risk assessment and spatial distribution of elemental concentrations in sediment
Authors: Ediagbonya T. F. , Balogun O. T.
Published: June 2020

Sediments are carrier and a sink of elements in the hydrological cycle. Monitoring of contaminated soil and sediment with metallic elements is of interest due to their influence on groundwater, surface water, plants, animals and humans. In this study, different sediment samples from five various places were taken, and the elemental concentration along with some physiochemical parameters were determined. The elemental concentrations were determined with proton-induced X-ray emission, while the physiochemical parameters were determined using a conductivity meter, pH meter and thermometer. This study showed that silicon had highest mean concentration and lead had the lowest mean concentration. Cadmium had the highest contamination values in all the locations. Okitipupa had the highest pollution load index, and the lowest pollution index was recorded in Irele. The overall risk index (RI) in all the locations in the sediment indicated very high ecological risk index. Pollution load index (PLI) also categorized ecological risk which ranged from 8.812 to 28.42. Mean PLI value (17.7) recorded in this study was far higher than the threshold (< 1), indicating the presence of heavy pollutant levels. Such high-level PLI values signified danger and measures are needed in order to reduce the sources of pollutants in the sediment. From enrichment value and Igeo-accumulation values, Sn was severely polluted. Si, Zr, Ag and Cd were extremely polluted. The physiochemical parameters correlated with the elemental concentrations both negatively and positively.
Polymorphism of TLR2 in bank vole populations in North Eastern Poland is not associated with Borrelia afzelii infection prevalence
Authors: Tarnowska Ewa , Niedziałkowska Magdalena , Stojak Joanna , Jędrzejewska Bogumiła
Published: June 2020

Polymorphism in innate immune genes in host populations can structure spatial variation in the prevalence of infectious diseases. In Europe,Borrelia afzeliiis an important tick-borne pathogen of small mammals including the bank voles (Myodes glareolus). The Toll-like receptor 2 (TLR2) is an innate immune receptor that is important for detectingBorrelia burgdorferisensulatopathogens. The TLR2 gene is polymorphic in bank vole populations and is classified into four distinct clusters: C1, C2, C3, and C4. The C2 and C4 clusters versus the C1 and C3 clusters are associated with lower versus higher infection prevalence, respectively. We detected three TLR2 clusters in 487 bank voles from 30 populations in NE Poland: 84.2% of the obtained sequences belonged to the C1 variant, 7.2% to C2, and 8.6% to C3. However, no clear spatial structure of TLR2 clusters among the populations was detected.B. afzeliiinfection prevalence across all studied individuals was 12.1% and varied from 0 to 37.5% among populations. There were no significant differences inB. afzeliiprevalence among voles carrying alleles of different TLR2 clusters, or between individuals belonging to two mtDNA lineages. Most infected individuals were adults, and males were infected more often than females. There was no significant relationship between the prevalence of TLR2 clusters in the vole populations and climatic and environmental factors within the study area. We therefore could not confirm an adaptive role of the TLR2 C2 alleles in reducingB. afzeliiinfection prevalence in bank voles.
Linkages Between Tropical Cyclones and Extreme Precipitation over China and the Role of ENSO
Authors: Wang Licheng , Yang Zhengnan , Gu Xihui , Li Jianfeng
Published: June 2020

This research investigated the linkages between tropical cyclones (TCs) and extreme precipitation, and their associations with El Niño-Southern Oscillation (ENSO) over China. The contribution of TC-induced to total extreme precipitation events along the southeast coast of China was higher than 50%, and the values gradually decreased as TCs moved inland. However, the precipitation extremes (magnitude and frequency) related to TCs did not show statistically significant changes over the most recent 57 years. The impacts of TCs on precipitation extremes are evidently modulated by the ENSO phases. We found less extreme precipitation linked with TCs in southeastern China during El Niño phase, because of the fewer TC tracks over this region and less TC genesis in the western North Pacific (WNP). The small TC track density over southeastern China is due to the prevalent westerly steering flow and abnormal integrated vapor transport from northern to southern China during El Niño years. Additionally, warmer sea surface temperature, more vigorous westerlies, larger vorticity in 250 hPa, and higher divergence in 850 hPa in an El Niño phase jointly displaced the mean genesis of the WNP TCs eastward and this led to fewer TCs passing through southeastern China.
SR4R: An Integrative SNP Resource for Genomic Breeding and Population Research in Rice
Authors: Yan Jun , Zou Dong , Li Chen , Zhang Zhang , Song Shuhui , Wang Xiangfeng
Published: June 2020

The information commons for rice (IC4R) database is a collection of 18 million single nucleotide polymorphisms (SNPs) identified by resequencing of 5152 rice accessions. Although IC4R offers ultra-high density rice variation map, these raw SNPs are not readily usable for the public. To satisfy different research utilizations of SNPs for population genetics, evolutionary analysis, association studies, and genomic breeding in rice, raw genotypic data of these 18 million SNPs were processed by unified bioinformatics pipelines. The outcomes were used to develop a daughter database of IC4R – SnpReady for Rice (SR4R). SR4R presents four reference SNP panels, including 2,097,405 hapmapSNPs after data filtration and genotype imputation, 156,502 tagSNPs selected from linkage disequilibrium-based redundancy removal, 1180 fixedSNPs selected from genes exhibiting selective sweep signatures, and 38 barcodeSNPs selected from DNA fingerprinting simulation. SR4R thus offers a highly efficient rice variation map that combines reduced SNP redundancy with extensive data describing the genetic diversity of rice populations. In addition, SR4R provides rice researchers with a web interface that enables them to browse all four SNP panels, use online toolkits, as well as retrieve the original data and scripts for a variety of population genetics analyses on local computers. SR4R is freely available to academic users at http://sr4r.ic4r.org/.
Predictors of success in hypoglossal nerve stimulator implantation for obstructive sleep apnea
Authors: Chao Tiffany N. , Thaler Erica R.
Published: June 2020

Objective

Current guidelines for hypoglossal nerve stimulator (HGNS) implantation eligibility include drug‐induced sleep endoscopy (DISE) findings and other patient characteristics but lead to highly variable rates of surgical success across institutions. Our objective was to determine whether additional factors seen on preoperative evaluation could be used as predictors of surgical success.

Study design

Retrospective chart review.

Setting

Single‐institution academic tertiary care medical center.

Subjects

and Methods:This study included patients with obstructive sleep apnea (OSA) who underwent HGNS implantation between 2015 and 2018. Surgical success was defined as a postoperative apnea‐hypopnea index (AHI) of less than 20 events per hour and an AHI reduction of at least 50%. Preoperative polysomnogram (PSG) results, DISE findings, and physical parameters were compared between surgical successes and failures.

Results

A total of 68 patients were included in the analysis. The overall surgical success rate was 79.4% (54/68). Elevated preoperative AHI was associated with an increased likelihood of treatment failure, with an AHI of (36.9 ± 16.8) events/hour in the success group compared to (49.4 ± 19.6) events/hour in the failure group (P = 0.05). Patients observed to have partial lateral oropharyngeal collapse on DISE was more frequently associated with the treatment failure group than in the success group (P = 0.04).

Conclusion

Patients who underwent HGNS implantation overall had a very high treatment response rate at our institution. Factors that may predispose patients to surgical failure included the presence of lateral oropharyngeal collapse and a significantly elevated preoperative AHI. These should be considered when determining surgical candidacy for HGNS implantation.
Demonstration of a prototype design synthesis capability for space access vehicle design
Authors: Rana L. , Chudoba B.
Published: June 2020

The early conceptual design (CD) phase of space access vehicles (SAVs) is the most abstract, innovative and technologically challenging phase of the entire aerospace design life cycle. Although the design decision-making during this phase influences around 80 percent of the overall life cycle cost, it is the most abstract and thus least understood phase of the entire design life cycle. The history of SAV design provides numerous examples of project failures that could have been avoided if the decision-maker had had the capability to forecast the potential risks and threats correctly ahead of time during the conceptual design phase. The present study addresses this crucial phase and demonstrates a best-practice synthesis methodology prototype to advance the current state of the art of CD as applied to SAV design. Developed by the Aerospace Vehicle Design (AVD) Laboratory at the University of Texas at Arlington (UTA), the Aerospace Vehicle Design Synthesis process and software (AVDS) is a prototype solution for a flight vehicle configuration–flexible (generic) design synthesis capability that can be applied to the primary categories of SAVs. This study focusses on introducing AVDS, followed by the demonstration and verification of the system’s capability through a sizing case study based on the data-rich Boeing X-20 Dyna-Soar spaceplane.
Interleukin-6 and total antioxidant capacity levels following N-acetylcysteine and a combination nutraceutical intervention in a randomised controlled trial for bipolar disorder
Authors: Bortolasci C.C. , Voigt C. , Turner A. , Mohebbi M. , Gray L. , Dodd S. , Walder K. , Berk M. , Cotton S.M. , Malhi G.S. , Ng C.H. , Dowling N. , Sarris J. , Dean O.M.
Published: June 2020

Objective:

The aims of this study were to evaluate changes in inflammatory and oxidative stress levels following treatment with N-acetylcysteine (NAC) or mitochondrial-enhancing agents (CT), and to assess the how these changes may predict and/or moderate clinical outcomes primarily the Montgomery-Åsberg Depression Rating Scale (MADRS).

Methods:

This study involved secondary analysis of a placebo-controlled randomised trial (n = 163). Serum samples were collected at baseline and week 16 of the clinical trial to determine changes in Interleukin-6 (IL-6) and total antioxidant capacity (TAC) following adjunctive CT and/or NAC treatment, and to explore the predictability of the outcome or moderator effects of these markers.

Results:

In the NAC-treated group, no difference was observed in serum IL-6 and TAC levels after 16 weeks of treatment with NAC or CT. However, results from a moderator analysis showed that in the CT group, lower IL-6 levels at baseline was a significant moderator of MADRS χ2 (df) = 4.90, p = 0.027) and Clinical Global Impression-Improvement (CGI-I, χ2 (df) = 6.28 p = 0.012). In addition, IL-6 was a non-specific but significant predictor of functioning (based on the Social and Occupational Functioning Assessment Scale (SOFAS)), indicating that individuals with higher IL-6 levels at baseline had a greater improvement on SOFAS regardless of their treatment (p = 0.023).

Conclusion:

Participants with lower IL-6 levels at baseline had a better response to the adjunctive treatment with the mitochondrial-enhancing agents in terms of improvements in MADRS and CGI-I outcomes.
Royal Free Hospital-Nutritional Prioritizing Tool improves the prediction of malnutrition risk outcomes in liver cirrhosis patients compared with Nutritional Risk Screening 2002
Authors: Wu Yuchao , Zhu Yage , Feng Yali , Wang Ruojing , Yao Naijuan , Zhang Mengmeng , Liu Xiaohui , Liu Huan , Shi Lei , Zhu Li , Yang Nan , Chen Hongmei , Liu Jinfeng , Zhao Yingren , Yang Yuan
Published: June 2020

The European Society for Clinical Nutrition and Metabolism (ESPEN) guidelines recommend the Royal Free Hospital-Nutritional Prioritizing Tool (RFH-NPT) to identify malnutrition risk in patients with liver disease. However, little is known about the application of the RFH-NPT to screen for the risk of malnutrition in China, where patients primarily suffer from hepatitis virus-related cirrhosis. A total of 155 cirrhosis patients without liver cancer or uncontrolled co-morbid illness were enrolled in this prospective study. We administered the Nutritional Risk Screening 2002 (NRS-2002), RFH-NPT, Malnutrition Universal Screening Tool (MUST) and Liver Disease Undernutrition Screening Tool (LDUST) to the patients within 24 h after admission and performed follow-up observations for 1·5 years. The RFH-NPT and NRS-2002 had higher sensitivities (64·8 and 52·4 %) and specificities (60 and 70 %) than the other tools with regard to screening for malnutrition risk in cirrhotic patients. The prevalence of nutritional risk was higher under the use of the RFH-NPT against the NRS-2002 (63 v. 51 %). The RFH-NPT tended more easily to detect malnutrition risk in patients with advanced Child–Pugh classes (B and C) and lower Model for End-stage Liver Disease scores (<15) compared with NRS-2002. RFH-NPT score was an independent predictive factor for mortality. Patients identified as being at high malnutrition risk with the RFH-NPT had a higher mortality rate than those at low risk; the same result was not obtained with the NRS-2002. Therefore, we suggest that using the RFH-NPT improves the ability of clinicians to predict malnutrition risk in patients with cirrhosis primarily caused by hepatitis virus infection at an earlier stage.
Diet and sedentary behaviour in relation to mortality in US adults with a cardiovascular condition: results from the National Health and Nutrition Examination Survey linked to the US mortality registry
Authors: Ricci Cristian , Leitzmann Michael F. , Freisling Heinz , Schutte Aletta E. , Schutte Rudolph , Kruger Salome H. , Smuts Cornelius M. , Pieters Marlien
Published: June 2020

CVD is the most common chronic condition and the highest cause of mortality in the USA. The aim of the present work was to investigate diet and sedentary behaviour in relation to mortality in US CVD survivors. The National Health and Nutrition Examination Surveys conducted between 1999 and 2014 linked to the US mortality registry updated to 2015 were investigated. Multivariate adjusted Cox regression was used to derive mortality hazards in relation to sedentary behaviour and nutrient intake. A multiplicative and additive interaction analysis was conducted to evaluate how sedentariness and diet influence mortality in US CVD survivors. A sample of 2473 participants followed for a median period of 5·6 years resulted in 761 deaths, and 199 deaths were due to CVD. A monotone increasing relationship between time spent in sedentary activities and mortality risk was observed for all-cause and CVD mortality (hazard ratio (HR) = 1·20, 95 % CI 1·09, 1·31 and HR = 1·19, 95 % CI 1·00, 1·67, respectively). Inverse mortality risks in the range of 22–34 % were observed when comparing the highest with the lowest tertile of dietary fibre, vitamin A, carotene, riboflavin and vitamin C. Sedentariness below 360 min/d and dietary fibre and vitamin intake above the median interact on an additive scale influencing positively all-cause and CVD mortality risk. Reduced sedentariness in combination with a varied diet rich in dietary fibre and vitamins appears to be a useful strategy to reduce all-cause and CVD mortality in US CVD survivors.
Effects of dietary carbohydrate sources on lipid metabolism and SUMOylation modification in the liver tissues of yellow catfish
Authors: Yang Shui-Bo , Zhao Tao , Wu Li-Xiang , Xu Yi-Chuang , Tan Xiao-Ying
Published: June 2020

Dysregulation in hepatic lipid synthesis by excess dietary carbohydrate intake is often relevant with the occurrence of fatty liver; therefore, the thorough understanding of the regulation of lipid deposition and metabolism seems crucial to search for potential regulatory targets. In the present study, we examined TAG accumulation, lipid metabolism-related gene expression, the enzyme activities of lipogenesis-related enzymes, the protein levels of transcription factors or genes involving lipogenesis in the livers of yellow catfish fed five dietary carbohydrate sources, such as glucose, maize starch, sucrose, potato starch and dextrin, respectively. Generally speaking, compared with other carbohydrate sources, dietary glucose promoted TAG accumulation, up-regulated lipogenic enzyme activities and gene expressions, and down-regulated mRNA expression of genes involved in lipolysis and small ubiquitin-related modifier (SUMO) modification pathways. Further studies found that sterol regulatory element binding protein 1 (SREBP1), a key transcriptional factor relevant to lipogenic regulation, was modified by SUMO1. Mutational analyses found two important sites for SUMOylation modification (K254R and K264R) in SREBP1. Mutant SREBP lacking lysine 264 up-regulated the transactivation capacity on an SREBP-responsive promoter. Glucose reduced the SUMOylation level of SREBP1 and promoted the protein expression of SREBP1 and its target gene stearoyl-CoA desaturase 1 (SCD1), indicating that SUMOylation of SREBP1 mediated glucose-induced hepatic lipid metabolism. Our study elucidated the molecular mechanism of dietary glucose increasing hepatic lipid deposition and found that the SREBP-dependent transactivation was regulated by SUMO1 modification, which served as a new target for the transcriptional programmes governing lipid metabolism.
Adelante!Military Imaginaries, the Cold War, and Southern Africa's Liberation Armies
Authors: Alexander Jocelyn , McGregor Joann
Published: June 2020

Studies of southern Africa's liberation movements have turned attention to the great importance of their transnational lives, but have rarely focused on the effects of the military training Cold War-era allies provided in sites across the globe. This is a significant omission in the history of these movements: training turns civilians into soldiers and creates armies with not only military but also social and political effects, as scholarship on conventional militaries has long emphasized. Liberation movement armies were however different in that they were not subordinated to a single state, instead receiving training under the flexible rubric of international solidarity in a host of foreign sites and in interaction with a great variety of military traditions. The training provided in this context produced multiple “military imaginaries” within liberation movement armies, at once creating deep tensions and enabling innovation. The article is based on oral histories of Zimbabwe People's Revolutionary Army (ZIPRA) veterans trained by Cuban and Soviet instructors in Angola in the late 1970s. These soldiers emerged from the Angolan camps with a military imaginary they summed up in the Cuban exhortation “Adelante!” (Forward!). Forty years later, they stressed how different their training had made them from other ZIPRA cadres, in terms of their military strategy, mastery of advanced Soviet weaponry, and aggressive disposition, as well as their “revolutionary” performance of politics and masculinity in modes of address, salute, and drill. Such military imaginaries powerfully shaped the southern African battlefield. They offer novel insight into the distinctive institutions, identities, and memories forged through Cold War-era military exchanges.