bluuebunny/crossref_metadata_2025 huggingface dataset:
On the Detection of Fracture within Vibrating Beams Traversed by a Moving Force
Authors: Dadoulis Georgios I. , Manolis George D.
Published: July 2022

In this work, we examine the influence of a crack in the span of a beam as it is being traversed by a point force with constant velocity. This problem presents two types of discontinuities: one spatial, where the crack is modelled as a discontinuity in the slope of the deflection curve of the beam, and a temporal one, with the former derived as the point force moves forward in time. The aim is to interpret time signals registered at a given node on the beam, either during the forced vibration or the free vibration regimes, by using the Gabor transform of the transient beam response so as to observe a pattern that alludes to the location of the discontinuity. Three analytical methods are examined, namely eigenvalue extraction, Laplace transformation and the transform matrix technique. A numerical example is presented using the Laplace transformation, where it is possible to detect the location of damage during the traverse of a point force across the bridge span. Validation studies of the methodology presented here can be conducted in the future, either through field measurements or through experimental setups, which constitutes an important step in realizing applications in structural health monitoring of civil engineering infrastructure.
Two Prism Critical Angle Refractometry with Attenuating Media
Authors: Koutsoumpos Spyridon , Giannios Panagiotis , Moutzouris Konstantinos
Published: July 2022

We present a concept that enables the determination of the complex refractive index of attenuating media from two critical angles, measured sequentially at two interfaces between a single sample and two different prisms. The proposed method is general in that it applies with s and p polarisation states, thus it is suited for the characterisation of isotropic as well as anisotropic media. Uncertainty analysis indicates that relative error in the determination of the real (imaginary) index can be less than 10−4 (in the order of 10%), respectively.
Unipolar Electrogram-Guided versus Lesion Size Index-Guided Catheter Ablation in Patients with Paroxysmal Atrial Fibrillation
Authors: Fu Guohua , He Bin , Wang Binhao , Feng Mingjun , Du Xianfeng , Liu Jing , Yu Yibo , Gao Fang , Zhuo Weidong , Xu Yi , Qi Yingbo , Chu Huimin
Published: July 2022

Background: This research explores the relationship between the unipolar electrogram (UP-EGM) and lesion size index (LSI) in different regions of continuous circular lesions (CCLs) and to assess the safety and efficacy of UP-EGM-guided versus LSI-guided radiofrequency catheter ablation (RFCA) in patients with paroxysmal atrial fibrillation (PAF). Methods: A total of 120 patients with drug-refractory PAF who underwent index RFCA were scheduled to be consecutively included from March 2020 to April 2021. All the patients were randomly divided 1:1 into two groups: the UP-EGM group and the LSI group. The first-pass PVI rate, acute PVI rate, and the sinus rhythm maintenance rate were compared. Results: A total of 120 patients with PAF were included in the study: the UP-EGM group (n = 60) and the LSI group (n = 60). All the LSI values in the UP-EGM group were less than those in the corresponding regions in the LSI group (all p < 0.001). There were no significant differences in the first-pass PVI rate and acute PVI rate between the two groups. After a mean follow-up period of 11.31 ± 1.70 months, the sinus rhythm maintenance rate in the UP-EGM group was comparable to that in the LSI group (90% vs. 91.7%, p = 0.752). Conclusion: UP-EGM-guided and LSI-guided RFCA are both effective and safe in patients with PAF. However, UP-EGM may be more suitable than LSI for guiding individual RFCA.
Irritable Bowel Syndrome: A Multifaceted World Still to Discover
Authors: Bassotti Gabrio
Published: July 2022

Irritable bowel syndrome (IBS) is considered the prototype of disorders of gut–brain interaction (DGBI), and it is defined, according to Rome IV criteria, by the presence of abdominal pain or discomfort associated with bowel movements or changes in bowel habits with features of abnormal defecation [...]
A New Postoperative Regimen after CXL and PRK Using Topical NSAID and Steroids on the Open Ocular Surface
Authors: Hafezi Farhad , Hillen Mark , Kollros Leonard , Tan Jerry , Awwad Shady T.
Published: July 2022

Corneal epithelium removal during photorefractive keratotomy (PRK), TransPRK, or corneal cross-linking (CXL) means that patients experience pain and inflammation after the procedure, which need to be carefully managed with topical drug regimens. One highly effective class of topical analgesics is non-steroidal anti-inflammatory drugs (NSAIDs), but these must be used carefully, as their use has been associated with delayed re-epithelialization and, in rare cases, corneal melting. However, our clinical experience has been that the concomitant use of topical corticosteroids obviates this risk. Here, we present a mechanistic explanation for our observations, our TransPRK and epithelium-off CXL protocols, and the postoperative medication regimens where topical NSAIDs are used in combination with topical steroid therapy during the first two postoperative days (where pain and inflammation levels are the highest). We detail the results of a single-center retrospective case analysis that examined eyes that underwent TransPRK (n = 301) or epithelium-off CXL (n = 576). Topical NSAID use in the first two postoperative days to control pain and inflammation after PRK/TransPRK or epithelium-off CXL, when used in combination with topical steroid therapy, does not appear to be associated with corneal melting or delayed epithelial healing. This approach may represent an improvement over current methods of handling post-surgical pain in procedures that require corneal epithelial debridement.
Impact of Cryopreservation of Peripheral Blood Stem Cells (PBSC) in Transplantation from Matched Unrelated Donor (MUD)
Authors: Facchin Gabriele , Savignano Chiara , Battista Marta Lisa , Isola Miriam , De Martino Maria , Petruzzellis Giuseppe , Rosignoli Chiara , Pizzano Umberto , Cerno Michela , De Cecco Giulia , Bertone Antonella , Barillari Giovanni , Fanin Renato , Patriarca Francesca
Published: July 2022

Background: Cryopreservation of PBSC for allogenic hematopoietic stem cell transplantation (allo-HSCT) was implemented due to the current Coronavirus 2019 pandemic. The impact of match unrelated donor (MUD) graft freezing on the outcome of allo-HSCT in terms of hematological recovery, graft versus host disease (GVHD), and survival are still controversial. Methods: In this study, we compared graft composition, clinical characteristics, and outcome of 31 allo-HSCT from MUD cryopreserved PBSC (Cryo Group) with 23 matched-pair allo-HSCT from fresh MUD PBSC (Fresh Group) performed in our center between January 2020 and July 2021. Results: No significant differences were recognized in clinical characteristics of patients, donors, and transplants between the Cryo and Fresh groups except for a better prognostic comorbidity index (HCT-CI) of the Cryo group. In the Cryo Group, the median time from apheresis to cryopreservation was 46.0 h (range 23.8–53.5), while the median time from cells collection and reinfusion was 13.9 days (range 5.8–28.1). In the Fresh Group, median time from apheresis to reinfusion was 35.6 h (range 21.4–51.2). The number of viable (7-AAD negative) CD34+ cells per kg patient infused was significantly lower in the Cryo Group (5.2 ± 1.9 × 106/kg vs. 7.0 ± 1.3 × 106/kg; p < 0.001). Indeed, there was a 36% (11–70) median loss of viable CD34+/kg cells after freezing. All patients engrafted: median time to neutrophil engraftment (>0.5 × 109/L) was 13.5 days (range 12–15) for Cryo Group and 14 days (range 13–16) days for Fresh Group (p = 0.522), while the median time to platelet engraftment (>20 × 109/L) was, respectively, 14 (range 12–18) and 15 (range 12–17) days (p = 0.904). The incidence of grade ≥ 2 acute GVHD was similar in the two groups (56.5% Cryo Group vs. 60.0% Fresh Group; p = 0.832) and no differences in terms of OS (p = 0.090), PFS (p = 0.200) and TRM (p = 0.970) were observed between the Cryo and Fresh groups. Conclusions: In our series, no differences between the Cryo and Fresh groups were found in engraftment, grade ≥ 2 acute GVHD incidence, OS, PFS, and TRM despite a lower CD34+ infused dose in the Cryo Group. Frozen PBSCs could be considered a safe option also for allo-HSCT from MUD but a higher amount of PBSC should be collected to warrant an adequate viable CD34+ post-thawing.
Assessment of Satisfaction, Compliance and Side Effects among Long-Term Orthokeratology Wearers
Authors: Wu Shang-Yen , Wang Jen-Hung , Chiu Cheng-Jen
Published: July 2022

Purpose: This study aims to assess the satisfaction, compliance, and side effects among the long-term orthokeratology (Ortho-K) users in a tertiary hospital in Taiwan and analyze the side effects and related risk factors. Methods: Children and their guardians were assessed using a structured and validated questionnaire inquiring about background information, wear and care behaviors, daily activities, satisfaction, and related concerns. Clinical information, including refractive data and side effects, was obtained through patient medical files. Results: Three hundred and five school-aged patients were enrolled, and the average age was 13.13 ± 3.39 years, with an average wearing period of 17.1 ± 8.1 months. Over 83% of the subjects had clear daytime vision all day, around 88% felt satisfied or very satisfied with the results, and 98% exhibited a willingness to continue wearing the Ortho-K lenses. Most guardians (83%) were pleased with the controlling effect of myopic progression. Initial spherical equivalent and regular cleaning of the lens protein significantly correlated with clear day vision. Wearing >6 days/week correlated with less risk of lens binding. Based on the questionnaire, the main reasons for using Ortho-K were effectiveness, safety, and practicality, while the major concerns were discomfort, harmful to the eyes, and no effect. Conclusion: With a comprehensive care program from practitioners and good compliance of users, Ortho-K could be the most effective and satisfactory option for myopic children in Taiwan.
Human Papilloma Virus Vaccination in Patients with Rheumatic Diseases in France: A Study of Vaccination Coverage and Drivers of Vaccination
Authors: David Emmanuelle , Roy Pascal , Belot Alexandre , Quartier Pierre , Bader Meunier Brigitte , Aeschlimann Florence A. , Lega Jean-Christophe , Durieu Isabelle , Rousset-Jablonski Christine
Published: July 2022

Objectives: To describe human papillomavirus (HPV) vaccination practices in adolescent girls with systemic lupus erythematosus (SLE) and juvenile idiopathic arthritis (JIA) and to identify barriers to and motivators for vaccination. Methods: Cross-sectional, multicenter study on girls aged 9 to 19 years and their accompanying adults. The measurement criteria were the proportion of girls who were vaccinated against HPV, compliance with the vaccination schedule, factors associated with vaccination, and reasons for vaccination and non-vaccination through a self-administered questionnaire. Results: Seventy-one patients (16 with SLE and 55 with JIA) were included with a mean age of 13 years old (rank 11–18). According to parental questioning, 39% of patients were vaccinated against HPV or in progress (44% and 38% of SLE and JIA, respectively). This rate was 82% for the 22 patients ≥ 15 years of age. The vaccine was administered as often by a general practitioner (39%) as by a hospital pediatrician (also 39%). Two factors were significantly associated with vaccination: Older age (OR 53.68, 95% CI 5.85–429.29, p < 0.001) and previous hepatitis B vaccination (OR 4.97, 95% CI 1.03–24.01, p = 0.040). Recommendation of the vaccine by a health professional and fear of HPV-related diseases were the main facilitators. Lack of knowledge about the vaccine, lack of recommendation by a health professional, and fear of vaccine side effects were the main barriers. Conclusions: HPV vaccination coverage remains insufficient among patients with autoimmune disease. Education and awareness of health professionals about HPV infections are crucial elements in vaccine acceptance.
Comparison of Fragmentation and Dusting Modality Using Holmium YAG Laser during Ureteroscopy for the Treatment of Ureteral Stone: A Single-Center’s Experience
Authors: Chen Bo-Han , Lin Tsu-Feng , Tsai Chih-Chun , Chen Marcelo , Chiu Allen W.
Published: July 2022

Laser ureteroscopic lithotripsy (URSL) is an efficacious treatment for ureteral stones. There have been few previous studies comparing the different energy and frequency settings for URSL in a single center. We compared these two laser modalities, which were simultaneously used in our medical center for the treatment of ureteral stones. Patients who underwent fragmentation or dusting laser URSL between September 2018 and June 2020 were retrospectively reviewed. We compared patients who underwent fragmentation and dusting laser and assessed the enhancing factors for stone free rate. There were a total of 421 patients with ureteral stones who met the study criteria. There was no significant difference between the characteristics of both groups. The fragmentation group had a better stone free rate and a lower retropulsion rate compared with the dusting group. Multivariate analysis revealed that stone basket use, no upper ureteral stone or pyuria significantly improved the stone free rate. Both laser modes were effective and safe for ureteral lithotripsy although the fragmentation system showed slightly higher effectiveness and lower complication rate.
Predictors of Anti-TNF Therapy Failure among Inflammatory Bowel Disease (IBD) Patients in Saudi Arabia: A Single-Center Study
Authors: Alharbi Othman , Aljebreen Abdulrahman M. , Azzam Nahla A. , Almadi Majid A. , Saeed Maria , HajkhderMullaissa Baraa , Asiri Hassan , Almutairi Abdullah , AlRuthia Yazed
Published: July 2022

Background: The advent of monoclonal antibodies (mAbs) has revolutionized the management of many immune-mediated diseases such as inflammatory bowel disease (IBD). Infliximab and adalimumab were the first mAbs approved for the management of IBD, and are still commonly prescribed for the treatment of both Crohn’s disease (CD) and ulcerative colitis (UC). Although mAbs have demonstrated high effectiveness rates in the management of IBD, some patients fail to respond adequately to mAbs, resulting in disease progression and the flare-up of symptoms. Objective: The objective was to explore the predictors of treatment failure among IBD patients on infliximab (INF) and adalimumab (ADA)—as demonstrated via colonoscopy with a simple endoscopic score (SES–CD) of ≥1 for CD and a Mayo score of ≥2 for UC—and compare the rates of treatment failure among patients on those two mAbs. Methods: This was a prospective cohort study among IBD patients aged 18 years and above who had not had any exposure to mAbs before. Those patients were followed after the initiation of biologic treatment with either INF or ADA until they were switched to another treatment due to failure of these mAbs in preventing the disease progression. Univariate and multiple logistic regressions were conducted to examine the predictors and rates of treatment failure. Results: A total of 146 IBD patients (118 patients on INF and 28 on ADA) met the inclusion criteria and were included in the analysis. The mean age of the patients was 31 years, and most of them were males (59%) with CD (75%). About 27% and 26% of the patients had penetrating and non-stricturing–non-penetrating CD behavior, respectively. Patients with UC had significantly higher odds of treatment failure compared to their counterparts with CD (OR = 2.58, 95% CI [1.06–6.26], p = 0.035). Those with left-sided disease had significantly higher odds of treatment failure (OR = 4.28, 95% CI [1.42–12.81], p = 0.0094). Patients on ADA had higher odds of treatment failure in comparison to those on INF (OR = 26.91, 95% CI [7.75–93.39], p = 0.0001). Conclusion: Infliximab was shown to be more effective in the management of IBD, with lower incidence rates of treatment failure in comparison to adalimumab.
Diagnostic Delay in Paediatric Inflammatory Bowel Disease—A Systematic Investigation
Authors: Khalilipour Bahareh Sophia , Day Andrew S. , Kenrick Kristin , Schultz Michael , Aluzaite Kristina
Published: July 2022

Diagnostic delays (time from the first symptoms to diagnosis) are common in inflammatory bowel disease (IBD) and may lead to worse disease progression and treatment outcomes. This study aimed to determine the duration of diagnostic delays (DD) and to explore associated factors in a cohort of children with IBD in New Zealand. In this study, patients with IBD diagnosed as children and their parents/caregivers completed questionnaires on the patients’ medical history, diagnostic experience, and demographic characteristics. The parent/caregiver questionnaire also included the Barriers to Care Questionnaire (BCQ). Patients’ healthcare data was reviewed to summarise the history of clinical visits and determine symptoms. Total DD, healthcare DD, patient DD and parent DD were derived from the primary dataset. Factors associated with the different types of DD were explored with a series of simple linear and logistical ordinal regressions. A total of 36 patients (Crohn’s disease 25, ulcerative colitis 10; male 17) were included. They were diagnosed at a median age of 12 years (interquartile range (IQR) 10–15 years). Total healthcare delay (from first healthcare visit to formal diagnosis) was median (IQR) 15.4 (6.5–34.2) months. The median (IQR) specialist-associated delay was 4.5 (0–34) days. Higher household income was associated with shorter healthcare delay (p < 0.018), while lower overall BCQ scores (indicating more barriers experienced) were associated with longer total healthcare DD. Higher scores in each subscale of BCQ (Skills; Pragmatics; Expectations; Marginalization; Knowledge and Beliefs) were also significantly associated with shorter total healthcare delay (p < 0.04). This study found substantial diagnostic delays in paediatric patients with IBD and identified significant associations between longer total healthcare diagnostic delays and overall household income and higher self-reported barriers to accessing healthcare.
A Heterologous V-01 or Variant-Matched Bivalent V-01D-351 Booster following Primary Series of Inactivated Vaccine Enhances the Neutralizing Capacity against SARS-CoV-2 Delta and Omicron Strains
Authors: Zhang Zhiren , He Qiaren , Zhao Wei , Li Yong , Yang Jiaming , Hu Zhenxiang , Chen Xi , Peng Hua , Fu Yang-Xin , Chen Long , Lu Ligong
Published: July 2022

Immune escape of emerging SARS-CoV-2 variants of concern (VOCs) and waning immunity over time following the primary series suggest the importance and necessity of booster shot of COVID-19 vaccines. With the aim to preliminarily evaluate the potential of heterologous boosting, we conducted two pilot studies to evaluate the safety and immunogenicity of the V-01 or a bivalent V-01D-351 (targeting Delta and Beta strain) booster after 5–7 months of the primary series of inactivated COVID-9 vaccine (ICV). A total of 77 participants were enrolled, with 20 participants in the V-01D-351 booster study, and 27, 30 participants in the age stratified participants of V-01 booster study. The safety results showed that V-01 or V-01D-351 was safe and well-tolerated as a heterologous booster shot, with overall adverse reactions predominantly being absent or mild in severity. The immunogenicity results showed that the heterologous prime–boost immunization with V-01 or bivalent V-01D-351 booster induced stronger humoral immune response as compared with the homologous booster with ICV. In particular, V-01D-351 booster showed the highest pseudovirus neutralizing antibody titers against prototype SARS-CoV-2, Delta and Omicron BA.1 strains at day 14 post boosting, with GMTs 22.7, 18.3, 14.3 times higher than ICV booster, 6.2, 6.1, 3.8 times higher than V-01 booster (10 μg), and 5.2, 3.8, 3.5 times higher than V-01 booster (25 μg), respectively. The heterologous V-01 booster also achieved a favorable safety and immunogenicity profile in older participants. Our study has provided evidence for a flexible roll-out of heterologous boosters and referential approaches for variant-specific vaccine boosters, with rationally conserved but diversified epitopes relative to primary series, to build herd immunity against the ongoing pandemic.
Association of Dry Eye Diseases and Auditory Sensitivity
Authors: Kim Kyung Wook , Hwang Jin Sun , Chang Jiwon , Shin Young Joo
Published: July 2022

This study aimed to evaluate the association between dry eye and inner ear diseases in a Korean population. Methods: Data from the Korean National Health and Nutrition Survey (KNHANES V, 2010–2012), a national cross-sectional health examination and survey, were collected by the Korea Centers for Disease Control and Prevention. The association between dry eye and inner ear disease was determined using the chi-square test and logistic regression analysis. The individuals were divided into two age groups (<60 and ≥60 years). Results: In total, 17,542 individuals (n = 11,932 in the <60 years group and n = 5610 in the ≥60 years group) were enrolled. After adjusting for confounding factors, the logistic regression model revealed that the associated factors were dizziness and loss of balance experience (OR, 1.315; 95% CI, 1.143–1.513), self-awareness of abnormal voice (OR, 1.372; 95% CI, 1.120–1.679), subjective hearing discomfort (OR, 1.278; CI, 1.084–1.506), and tinnitus (OR, 1.265; 95% CI, 1.101–1.453). The inversely associated factor for dry eye was bilateral hearing loss (OR, 0.497; 95% CI, 0.367–0.672). The hearing threshold was lower in the dry eye group than in the non-dry eye group (p < 0.05). Conclusions: Tinnitus was associated with dry eye and bilateral hearing loss was inversely associated with dry eye. These results suggest that hypersensitivity of the senses and nerves, which is neuropathic hyperesthesia, is one of the main mechanisms of dry eye. Treatment of neuropathy may help in treating dry eye associated with dizziness or tinnitus.
An Experimental Study on Electrical Properties of Self-Sensing Mortar
Authors: Durairaj Ramkumar , Varatharajan Thirumurugan , Srinivasan Satyanarayanan Kachabeswara , Gurupatham Beulah Gnana Ananthi , Roy Krishanu
Published: July 2022

Self-sensing cementitious composites are a combination of conventional materials used in the construction industry along with any type of electrically conductive filler material. Research has already been carried out with various types of conductive fillers incorporated into cement mortars to develop a self-sensing material. Carbon fibres have been used as conductive fillers in the past, which is uneconomical. In order to overcome this drawback, brass fibres have been introduced. This study concentrates on the behaviour of self-sensing mortar under two different curing conditions, including air and water curing. The main aim of this paper is to determine the self-sensing ability of various types of smart mortars. For this purpose, an experimental study was carried out, with the addition of various brass fibres of 0.10%, 0.15%, 0.20%, 0.25%, and 0.30% by volume, to determine the electrical properties of cementitious mortar. In addition, different combinations of brass and carbon fibres were considered, such as 95% brass fibre with 5% carbon fibre, 90% brass fibre with 10% carbon fibre, and 85% brass fibre with 15% carbon fibre by volume, to determine the piezoresistive behaviour. A fractional change in electrical resistance was determined for all the mortar cubes. A fractional change in electrical resistance (fcr) is defined as the change in its electrical resistance with respect to its initial resistance (ΔR/R). Additionally, the temperature effects on self-sensing mortar under compressive loading were observed for various temperatures from room temperature to 800 °C (at room temperature, 200 °C, 400 °C, 600 °C, and 800 °C). It was observed that the addition of brass fibre to the cement mortar as an electrically conductive filler improved the self-sensing ability of the mortar. After 28 days of water curing, when compared to conventional mortar, the percentage increase in change in electrical resistance (fcr) was observed to be 26.00%, 26.87%, 27.87%, 38.55%, and 35.00% for 0.10%, 0.15%, 0.20%, 0.25%, and 0.30% addition of brass fibres, respectively. When the smart mortar was exposed to elevated temperatures, the compressive strength of the mortar was reduced. Additionally, the fractional change in electrical resistance values was also reduced with the increase in temperature. In addition to this, the self-sensing ability of smart mortars showed improved performance in water curing rather than in air-cured mortars. Compressive strengths, stress, strain, and change in electrical resistance (fcr) values were determined in this study. Finally, microstructural analysis was also performed to determine the surface topography and chemical composition of the mortar with different fibre combinations.
Effectiveness of the Speed Reduction Strategy on Exhaust Emissions and Fuel Oil Consumption of a Marine Generator Engine for DC Grid Ships
Authors: Pham Van Chien , Kim Hanseok , Choi Jae-Hyuk , Nyongesa Antony J. , Kim Jongsu , Jeon Hyeonmin , Lee Won-Ju
Published: July 2022

Recent developments in power electronics, energy storage systems, and renewable energy; increased market demands for more efficient and cleaner electric power to meet stricter environmental regulations; and development in gigawatt (GW)-class DC (direct current) transmission systems for transmission of greater power over longer distances than similar alternative current (AC) systems, have supported the development of the DC grid, making it a promising solution for both the onshore and offshore industries. This paper presents an experimental study on the effectiveness of an engine speed reduction strategy on exhaust gas emission and fuel consumption when applied to a 4-stroke generator engine equipped with a cam-driven plunger diesel injection system. The experiments were performed on an 8-cylinder V-type 4-stroke generator engine installed in the MASTC laboratory, which is the only demonstration testbed for the ship’s electric propulsion system in Korea. Experimental results showed that fuel consumption decreased, but emission mass fraction in exhaust gas increased when maintaining engine power while reducing engine speed. This study has shown economic benefits in reducing fuel consumption, but incurred penalties for the emission performance of 4-stroke generator engines equipped with cam-driven plunger diesel injection systems when applying the engine speed reduction strategy.
Diversity of Colletotrichum Species Causing Apple Bitter Rot and Glomerella Leaf Spot in China
Authors: Chen Yang , Fu Dandan , Wang Wei , Gleason Mark L. , Zhang Rong , Liang Xiaofei , Sun Guangyu
Published: July 2022

Bitter rot and Glomerella leaf spot (GLS) of apples, caused by Colletotrichum species, are major diseases of apples around the world. A total of 98 isolates were obtained from apple fruits with bitter rot, and 53 isolates were obtained from leaves with leaf spot in the primary apple production regions in China. These isolates were characterized morphologically, and five gene regions (ITS, ACT, GAPDH, CHS-1 and TUB2) were sequenced for each isolate. A phylogenetic analysis, combined with a comparison of the morphological, cultural and pathogenic characters, sorted bitter rot isolates into six species: C. alienum, C. fructicola, C. gloeosporioides sensu stricto, C. nymphaeae, C. siamense and one new species, C. orientalis Dandan Fu & G.Y. Sun. Among these, C. siamense was the predominant pathogen associated with bitter rot. Isolates from leaf spot were identified as two species, C. aenigma and C. fructicola. This is the first report of C. orientalis as an apple bitter rot pathogen worldwide, and the results provide important insights into the diversity of Colletotrichum species in China.
Cell Population Dynamics in Wound-Induced Hair Follicle Neogenesis Model
Authors: Helm Maria , Loui Juliane , Simon Jan C. , Ferrer Ruben A.
Published: July 2022

Hair follicle (HF) regeneration can be achieved in the center of large full-thickness wounds on mouse backs (wound-induced HF neogenesis model, WIHN). Investigations with this model have allowed for the identification of some of the factors limiting the extent of fibrosis, which creates a permissive environment for the reposition of HF. For WIHN, specific subpopulations of cells rather than cell types are permissive to this process. Detailed information on the cellular composition in WIHN is not available. Here, we provide a description of changes in cell numbers of fibroblasts, HF dermal papilla, endothelial cells, keratinocytes (interfollicular epidermis, HF-infundibulum, HF-isthmus, HF-bulge (basal and suprabasal), HF-hair germ) and immune cells (macrophages, monocytes, dendritic cells, T cells (CD4+, CD8+, CD4+/CD8+, regulatory T cells) and neutrophils) based on flow cytometric analysis. We compared unwounded skin with large wounds (1.5 × 1.5 cm) at different time points after wounding. We found that non-immune dermal cells have the largest share in the skin at all time points studied, and that the number of epidermal cells started increasing nine days after wounding, which precede isthmus cells and bulge cells, mirroring the development of hair follicles. Monocytes and neutrophils represent most myeloid cells in wounds and remain in wounds even beyond the inflammatory phase of wound healing. Macrophages can be identified as inflammatory and alternative cells and are also found in wounds even in the late remodeling phase of wound healing. Lastly, we provide information about T cells in large wounds. Most T cells in the wounds were CD8+ at all time points and expressed γδTCR, which was previously thought to be expressed mainly on CD4+. We also report the existence of double positive CD4/CD8. Our study provides a guide in terms of time points suitable for the further study of cell subpopulations aiming to dissect the cellular heterogeneity in WIHN. Our results might set the base for the comparison of WIHN between control mice and animals manipulated to influence HF neogenesis and the full understanding of the responsible actors allowing for HF regeneration.
Association between Usual Dietary Intake of Food Groups and DNA Methylation and Effect Modification by Metabotype in the KORA FF4 Cohort
Authors: Hellbach Fabian , Baumeister Sebastian-Edgar , Wilson Rory , Wawro Nina , Dahal Chetana , Freuer Dennis , Hauner Hans , Peters Annette , Winkelmann Juliane , Schwettmann Lars , Rathmann Wolfgang , Kronenberg Florian , Koenig Wolfgang , Meisinger Christa , Waldenberger Melanie , Linseisen Jakob
Published: July 2022

Associations between diet and DNA methylation may vary among subjects with different metabolic states, which can be captured by clustering populations in metabolically homogenous subgroups, called metabotypes. Our aim was to examine the relationship between habitual consumption of various food groups and DNA methylation as well as to test for effect modification by metabotype. A cross-sectional analysis of participants (median age 58 years) of the population-based prospective KORA FF4 study, habitual dietary intake was modeled based on repeated 24-h diet recalls and a food frequency questionnaire. DNA methylation was measured using the Infinium MethylationEPIC BeadChip providing data on >850,000 sites in this epigenome-wide association study (EWAS). Three metabotype clusters were identified using four standard clinical parameters and BMI. Regression models were used to associate diet and DNA methylation, and to test for effect modification. Few significant signals were identified in the basic analysis while many significant signals were observed in models including food group-metabotype interaction terms. Most findings refer to interactions of food intake with metabotype 3, which is the metabotype with the most unfavorable metabolic profile. This research highlights the importance of the metabolic characteristics of subjects when identifying associations between diet and white blood cell DNA methylation in EWAS.
Clinical Validation of DNA Extraction-Free qPCR, Visual LAMP, and Fluorescent LAMP Assays for the Rapid Detection of African Swine Fever Virus
Authors: Yang Lili , Wang Lin , Lv Meihui , Sun Yu , Cao Jijuan
Published: July 2022

The global pig industry and food safety are seriously threatened by outbreaks of African swine fever (ASF). To permit early diagnosis of African swine fever virus (ASFV), prevent its spread, and limit its outbreaks, a highly sensitive diagnostic method that can be performed at pig farms is required. Herein, we established DNA extraction-free real-time PCR (qPCR), visual loop-mediated isothermal amplification (LAMP), and fluorescent LAMP assays, which were compared with the results of World Organization for Animal Health (OIE) qPCR to assess ASFV-infected clinical samples. Based on plasmid DNA, the limit of detection for the three assays and OIE qPCR were 5.8 copies/μL. All four assays had good ASFV specificity and showed no cross-reactivity with other tested viruses. These assays were used to diagnose 100 clinical samples. The assays showed good diagnostic consistency, with kappa values of 1.0, 0.84, and 0.88, respectively. Compared with OIE qPCR, the diagnostic specificity/sensitivity of DNA extraction-free qPCR, visual LAMP, and fluorescent LAMP assays were 100%/100%, 100%/87.1%, and 100%/90.32%, respectively. The assays eliminated the need for DNA extraction and are more suitable for ASF diagnosis by inexperienced farmers in low-resource environments, making them a good choice for on-site monitoring of pig farms.
Ultrasound Measurements of Rectus Femoris and Locomotor Outcomes in Patients with Spinal Cord Injury
Authors: Tay Matthew Rong Jie , Kong Keng He
Published: July 2022

Patients with incomplete spinal cord injury have decreased mobility, and many do not recover walking ability. The purpose of this study was to investigate rectus femoris muscle thickness and echo intensity on ultrasound and functional outcomes in these patients. This was a prospective cohort study in an inpatient rehabilitation center, which recruited 40 consecutive patients with incomplete spinal cord injury. The patients underwent an ultrasound assessment at 6 weeks post-injury. Ultrasound measurements were performed using B-mode ultrasound scanning and standardized protocols. Functional outcomes on discharge, including Lower Extremity Muscle Score (LEMS), Functional Independence Measure (FIM), and Walking Index for Spinal Cord Injury II (WISCI II), were measured. Rectus femoris muscle thickness was significantly correlated with discharge LEMS (Spearman’s rho = 0.448; p = 0.004), FIM motor subscale (Spearman’s rho = 0.595; p < 0.001), FIM walk subscale (Spearman’s rho = 0.621; p < 0.001) and WISCI II (Spearman’s rho = 0.531; p < 0.001). The rectus femoris echo intensity was also significantly correlated with discharge LEMS (Spearman’s rho = −0.345; p = 0.029), FIM motor subscale (Spearman’s rho = −0.413; p = 0.008), FIM walk subscale (Spearman’s rho = −0.352; p = 0.026), and WISCI II (Spearman’s rho = −0.355; p = 0.025). We report that a relationship exists between rectus femoris muscle ultrasonographic characteristics and muscle function and ambulatory outcomes after inpatient rehabilitation. Ultrasound muscle measurements are potentially useful in assessing muscle wasting and function in patients with spinal cord injury.
Emulsion Explosives: A Tutorial Review and Highlight of Recent Progress
Authors: Kramarczyk Bartlomiej , Suda Krystyna , Kowalik Patrycja , Swiatek Kuba , Jaszcz Katarzyna , Jarosz Tomasz
Published: July 2022

Emulsion explosives (EE) have been commercially available in various forms for over 50 years. Over this period, the popularity and production technology of this class of energetic materials have been developing constantly. Despite this rapid rise to prominence and, in some applications, prevalence over traditional energetic materials, remarkably little information is available on the physicochemical and energetic properties of these materials and factors affecting those properties. This work is dedicated to presenting the fundamental information relevant to the features, properties and applications of EEs, while highlighting the most significant recent progress pertaining to those materials. Particular emphasis has been given to providing information about the types, composition, modifications and detonation parameters of EEs, as well as to highlighting the less obvious, emerging applications of EEs.
Interatomic Potential for InP
Authors: Chrobak Dariusz , Majtyka-Piłat Anna , Ziółkowski Grzegorz , Chrobak Artur
Published: July 2022

Classical modeling of structural phenomena occurring in InP crystal, for example plastic deformation caused by contact force, requires an interatomic interaction potential that correctly describes not only the elastic properties of indium phosphide but also the pressure-induced reversible phase transition B3↔B1. In this article, a new parametrization of the analytical bond-order potential has been developed for InP. The potential reproduces fundamental physical properties (lattice parameters, cohesive energy, stiffness coefficients) of the B3 and B1 phases in good agreement with first-principles calculations. The proposed interaction model describes the reversibility of the pressure-induced B3↔B1 phase transition as well as the formation of native point defects in the B3 phase.
Effects of Selected Pigments on the Properties of Silicone Resin-Based Paints
Authors: Lisowski Jakub , Szadkowski Bolesław , Marzec Anna
Published: July 2022

The purpose of this research was to evaluate the impact of selected pigments on the performance of waterborne emulsion paint. Each pigment was incorporated into the paint at 5% w/w. Density and viscosity measurements as well as the rub-out test were used to test the wet state properties of the colored paint. Wet-scrub, adhesion-to-substrate, water-uptake, vapor-permeability, UV-aging, and other tests were conducted to evaluate effects of the pigments on the dried paint. Bohemian green earth pigment was found to have the most positive effect, as it improved the water resistance of the paint without changing its rheological properties. Therefore, this pigment was selected for further studies, in which the pigment was included as part of the paint formula rather than as a post-additive. The results were satisfactory, confirming the compatibility of the pigment with the formula. However, a slight change in the rheological profile of the paint was observed during tests on a rotational rheometer. This research shows the need for intensive quality control measures while testing alternative formulations, to both enable early detection of negative effects and identify possible improvements.
Application of Genetically Encoded Photoconvertible Protein SAASoti for the Study of Enzyme Activity in a Single Live Cell by Fluorescence Correlation Microscopy
Authors: Solovyev Ilya D. , Maloshenok Liliya G. , Savitsky Alexander P.
Published: July 2022

Fluorescent Correlation Spectroscopy (FCS) allows us to determine interactions of labeled proteins or changes in the oligomeric state. The FCS method needs a low amount of fluorescent dye, near nanomolar concentrations. To control the amount of fluorescent dye, we used new photoconvertible FP SAASoti. This work is devoted to the proof of principle of using photoconvertible proteins to measure caspase enzymatic activity in a single live cell. The advantage of this approach is that partial photoconversion of the FP makes FCS measurements possible when studying enzymatic reactions. To investigate the process, in vivo we used HeLa cell line expressing the engineered FRET sensor, SAASoti-23-KFP. This FRET sensor has a cleavable (DEVD) sequence in the linker between two FPs for the detection of one of the key enzymes of apoptosis, caspase-3. Caspase-3 activity was detected by registering the increase in the fluorescent lifetimes of the sensor, whereas the diffusion coefficient of SAASoti decreased. This can be explained by an increase in the total cell viscosity during apoptosis. We can suppose that in the moment of detectible caspase-3 activity, cell structure already has crucial changes in viscosity.
The New High-Pressure Phases of Nitrogen-Rich Ag–N Compounds
Authors: Liu Ran , Xu Dan , Yao Zhen , Niu Shifeng , Liu Bingbing
Published: July 2022

The high-pressure phase diagram of Ag–N compounds is enriched by proposing three stable high-pressure phases (P4/mmm-AgN2, P1-AgN7 and P-1-AgN7) and two metastable high-pressure phases (P-1-AgN4 and P-1-AgN8). The novel N7 rings and N20 rings are firstly found in the folded layer structure of P-1-AgN7. The electronic structure properties of predicted five structures are studied by the calculations of the band structure and DOS. The analyses of ELF and Bader charge show that the strong N–N covalent bond interaction and the weak Ag–N ionic bond interaction constitute the stable mechanism of Ag–N compounds. The charge transfer between the Ag and N atoms plays an important role for the structural stability. Moreover, the P-1-AgN7 and P-1-AgN8 with the high-energy density and excellent detonation properties are potential candidates for new high-energy density species.
The Effect of Grain Size on the Diffusion Efficiency and Microstructure of Sintered Nd-Fe-B Magnets by Tb Grain Boundary Diffusion
Authors: Guo Shuai , Yang Xiao , Fan Xiaodong , Ding Guangfei , Cao Shuai , Zheng Bo , Chen Renjie , Yan Aru
Published: July 2022

The grain boundary diffusion process (GBDP) of heavy rare earth Tb is an effective method to improve the coercivity of Nd-Fe-B magnets, and the matrix grain size has a crucial effect on the diffusion efficiency and depth of the Tb element. In this work, magnets with different grain sizes have been fabricated using powder metallurgy to investigate the effect of grain size on Tb diffusion efficiency and the microstructure of Nd-Fe-B-type magnets. After the Tb diffusion process, the coercivity increment of the magnet with 4.9 μm large grain is 8.60 kOe, which is much higher than that of the magnet with 3.0 μm small grain (~5.90 kOe), which clearly demonstrates that the coercivity increment decreases as the grain size decreases. Microstructure analysis suggested that grain refinement significantly increases the total surface area, resulting in narrowing and discontinuity of the grain boundary phase (GBP). Therefore, as the channel for diffusion, the narrowing and discontinuity of the GBP are unfavorable for diffusion, resulting in a decrease in diffusion efficiency.
A Review on Recycling of Carbon Fibres: Methods to Reinforce and Expected Fibre Composite Degradations
Authors: Isa Amiruddin , Nosbi Norlin , Che Ismail Mokhtar , Md Akil Hazizan , Wan Ali Wan Fahmin Faiz , Omar Mohd Firdaus
Published: July 2022

Carbon fibres are widely used in modern industrial applications as they are high-strength, light in weight and more reliable than other materials. The increase in the usage of carbon fibres has led to the production of a significant amount of waste. This has become a global issue because valuable carbon fibre waste ends up in landfill. A few initiatives have been undertaken by several researchers to recycle carbon fibre waste; however, the properties of this recycled material are expected to be worse than those of virgin carbon fibre. The incorporation of polymers, nanoparticles and other hybrid materials could enhance the overall properties of recycled carbon fibre waste. However, the degradation of fibre composites is expected to occur when the material is exposed to certain conditions and environments. The study of fibre composite degradation is crucial to enhance their properties, strength, safety and durability for future applications.
Experimental Analysis of the Stress State of a Prestressed Cylindrical Shell with Various Structural Parameters
Authors: Zhangabay Nurlan , Sapargaliyeva Bayan , Utelbayeva Akmaral , Kolesnikov Alexandr , Aldiyarov Zhumadilla , Dossybekov Serik , Esimov Esenbek , Duissenbekov Bolat , Fediuk Roman , Vatin Nikolai , Yermakhanov Myrzabek , Mussayeva Saule
Published: July 2022

The paper presents the results of experimental studies of the features of the operation of prestressed shells, taking into account the various structural parameters of the prestress. It is established that when the winding angle changes from perpendicular to the shell axis to 75° and 65°, the circumferential stresses decrease 1.4 times and 1.2 times, respectively, and the axial stresses increase five and three times, which are two and four times lower than the circumferential, from which it can be concluded that the reduction in the winding angle to the longitudinal the axis of the shell has a positive effect on the stress state of the structure. The study also found that with an increase in the diameter of the winding wire from 1 to 2 mm and a change in the winding angle, the same nature of the stress distribution is observed, but the values of the stress state parameter change, so the efficiency increases up to 25% due to an increase in the winding thickness, depending on the pitch, angle and thickness of the winding, which favorably affects the strength and the bearing capacity of the structure as a whole by increasing the value of the stress state parameter. Thus, the results of the analysis will allow us to use in more detail the possibility of controlling the stress–strain state of the prestressed shell by changing the design parameters, and the results obtained can be used in design or construction, as well as when increasing the strength characteristics of the structure, which allows us to create a high-tech design optimal for these operating conditions, which can positively complement the studies conducted earlier in this direction.
Predicting Elastic Constants of Refractory Complex Concentrated Alloys Using Machine Learning Approach
Authors: Bhandari Uttam , Ghadimi Hamed , Zhang Congyan , Yang Shizhong , Guo Shengmin
Published: July 2022

Refractory complex concentrated alloys (RCCAs) have drawn increasing attention recently owing to their balanced mechanical properties, including excellent creep resistance, ductility, and oxidation resistance. The mechanical and thermal properties of RCCAs are directly linked with the elastic constants. However, it is time consuming and expensive to obtain the elastic constants of RCCAs with conventional trial-and-error experiments. The elastic constants of RCCAs are predicted using a combination of density functional theory simulation data and machine learning (ML) algorithms in this study. The elastic constants of several RCCAs are predicted using the random forest regressor, gradient boosting regressor (GBR), and XGBoost regression models. Based on performance metrics R-squared, mean average error and root mean square error, the GBR model was found to be most promising in predicting the elastic constant of RCCAs among the three ML models. Additionally, GBR model accuracy was verified using the other four RHEAs dataset which was never seen by the GBR model, and reasonable agreements between ML prediction and available results were found. The present findings show that the GBR model can be used to predict the elastic constant of new RHEAs more accurately without performing any expensive computational and experimental work.