bluuebunny/crossref_metadata_2025 huggingface dataset:
Native Bamboo Invasions into Subtropical Forests Alter Microbial Communities in Litter and Soil
Authors: Tian Xiao-Kun , Wang Min-Yan , Meng Ping , Zhang Jin-Song , Zhou Ben-Zhi , Ge Xiao-Gai , Yu Fei-Hai , Li Mai-He
Published: March 2020

Both exotic and native plant invasions can have profound impacts on ecosystems. While many studies have examined the effects of exotic plant invasions on soil properties, relatively few have tested the effects of native plant invasions on soil microbial communities. Furthermore, we know little about the effects of native plant invasions on microbial communities in litter. In subtropical forests in southern China, we sampled litter at three decomposition stages and top soil in three forest sands representing three stages of the invasion (not invaded, moderately and heavily invaded) by the Moso bamboo (Phyllostachys edulis (Carriere) J. Houzeau), a native species in China. We measured chemical properties (concentrations of C, N, P, Mg, Al, K, Ca, Mn, Cu, and Zn, and concentrations of cellulose and lignin) and microbial communities in litter and/or soil. The bamboo invasion, in general, decreased the element concentrations in litter and soil and also decreased total microbial abundance and diversity. Considering bacteria and fungi separately, the bamboo invasion decreased fungal diversity in litter and soil, but had little impact on bacterial diversity, suggesting that fungi are more sensitive and vulnerable to the bamboo invasion than bacteria. We conclude that native Moso bamboo invasions into subtropical forests may lead to a complex biogeochemical process in the litter–soil system, which may threaten local forest ecosystems by affecting microbial communities and, thus, litter decomposition and nutrient cycling.
Comprehensive Analysis of the TIFY Gene Family and Its Expression Profiles under Phytohormone Treatment and Abiotic Stresses in Roots of Populus trichocarpa
Authors: Wang Hanzeng , Leng Xue , Xu Xuemei , Li Chenghao
Published: March 2020

The TIFY gene family is specific to land plants, exerting immense influence on plant growth and development as well as responses to biotic and abiotic stresses. Here, we identify 25 TIFY genes in the poplar (Populus trichocarpa) genome. Phylogenetic tree analysis revealed these PtrTIFY genes were divided into four subfamilies within two groups. Promoter cis-element analysis indicated most PtrTIFY genes possess stress- and phytohormone-related cis-elements. Quantitative real-time reverse transcription polymerase chain reaction (qRT–PCR) analysis showed that PtrTIFY genes displayed different expression patterns in roots under abscisic acid, methyl jasmonate, and salicylic acid treatments, and drought, heat, and cold stresses. The protein interaction network indicated that members of the PtrTIFY family may interact with COI1, MYC2/3, and NINJA. Our results provide important information and new insights into the evolution and functions of TIFY genes in P. trichocarpa.
Diagnosing Magnetic Field Geometry in Blazar Jets Using Multi-Frequency, Centimeter-Band Polarimetry and Radiative Transfer Modeling
Authors: Aller Margo , Hughes Philip , Aller Hugh , Hovatta Talvikki
Published: March 2020

We use multi-frequency linear polarization observations from the University of Michigan blazar program (UMRAO), in combination with radiative transfer simulations of emission from a relativistic jet, to investigate the time-dependent flow conditions, including magnetic field geometry, in an example blazar OT 081. We adopt a scenario incorporating relativistic shocks during flaring, and both ordered axial and helical magnetic field components and magnetic turbulence in the underlying flow; these constituents are consistent with the observed periods of ordered behavior in the polarization intermixed with stochastic variations. The simulations are able to reproduce the global features of the observed light curves, including amplitude and spectral evolution of the linear polarization, during four time periods spanning 25 years. From the simulations, we identify the signature of a weak-to-strong helical magnetic field on the polarization, but conclude that a dominant helical magnetic field is not consistent with the UMRAO polarization data. The modeling identifies time-dependent changes in the ratio of the ordered-to-turbulent magnetic field, and changes in the flow direction and Lorentz factor. These suggest the presence of jet-like structures within a broad envelope seen at different orientations.
Molecular Analysis of the Official Algerian Olive Collection Highlighted a Hotspot of Biodiversity in the Central Mediterranean Basin
Authors: Haddad Benalia , Gristina Alessandro Silvestre , Mercati Francesco , Saadi Abd Elkader , Aiter Nassima , Martorana Adriana , Sharaf Abdoallah , Carimi Francesco
Published: March 2020

Genetic diversity and population structure studies of local olive germplasm are important to safeguard biodiversity, for genetic resources management and to improve the knowledge on the distribution and evolution patterns of this species. In the present study Algerian olive germplasm was characterized using 16 nuclear (nuSSR) and six chloroplast (cpSSR) microsatellites. Algerian varieties, collected from the National Olive Germplasm Repository (ITAFV), 10 of which had never been genotyped before, were analyzed. Our results highlighted the presence of an exclusive genetic core represented by 13 cultivars located in a mountainous area in the North-East of Algeria, named Little Kabylie. Comparison with published datasets, representative of the Mediterranean genetic background, revealed that the most Algerian varieties showed affinity with Central and Eastern Mediterranean cultivars. Interestingly, cpSSR phylogenetic analysis supported results from nuSSRs, highlighting similarities between Algerian germplasm and wild olives from Greece, Italy, Spain and Morocco. This study sheds light on the genetic relationship of Algerian and Mediterranean olive germplasm suggesting possible events of secondary domestication and/or crossing and hybridization across the Mediterranean area. Our findings revealed a distinctive genetic background for cultivars from Little Kabylie and support the increasing awareness that North Africa represents a hotspot of diversity for crop varieties and crop wild relative species.
Cell Progeny in the Olfactory Bulb after Targeting Specific Progenitors with Different UbC-StarTrack Approaches
Authors: Sánchez-González Rebeca , Figueres-Oñate María , Ojalvo-Sanz Ana Cristina , López-Mascaraque Laura
Published: March 2020

The large phenotypic variation in the olfactory bulb may be related to heterogeneity in the progenitor cells. Accordingly, the progeny of subventricular zone (SVZ) progenitor cells that are destined for the olfactory bulb is of particular interest, specifically as there are many facets of these progenitors and their molecular profiles remain unknown. Using modified StarTrack genetic tracing strategies, specific SVZ progenitor cells were targeted in E12 mice embryos, and the cell fate of these neural progenitors was determined in the adult olfactory bulb. This study defined the distribution and the phenotypic diversity of olfactory bulb interneurons from specific SVZ-progenitor cells, focusing on their spatial pallial origin, heterogeneity, and genetic profile.
Methods of Disinfecting Stethoscopes: Systematic Review
Authors: Napolitani Margherita , Bezzini Daiana , Moirano Fulvio , Bedogni Corrado , Messina Gabriele
Published: March 2020

The aim of this systematic review was to investigate the effectiveness of various disinfection methods available for stethoscopes. In March 2019, we performed a search in PubMed and Scopus using the search terms: “reducing stethoscopes contamination” and “disinfection stethoscopes”; the Mesh terms used in PubMed were “Decontamination/methods” or “Disinfection/methods” and “Stethoscopes/microbiology”. Selection criteria were: English language; at least one disinfection method tested. A total of 253 publications were screened. After title, abstract, and full-text analysis, 17 papers were included in the systematic review. Ethanol at 90%, Ethanol-Based Hands Sanitizer (EBHS), triclosan, chlorhexidine, isopropyl alcohol, 66% ethyl alcohol, sodium hypochlorite, and benzalkonium chloride have been proven to lower the presence of bacteria on stethoscopes’ surfaces. In addition, alcohol wipes show effective results. A wearable device emitting ultraviolet C by Light-Emitting Diode (LED) resulted efficacious against common microorganisms involved in Healthcare Associated Infections. The cover impregnated with silver ions seemed to be associated with significantly higher colony counts. Instead, copper stethoscopes surface reduced bacterial load. The disinfection of stethoscopes appears to be essential. There are many valid methods available; the choice depends on various factors, such as the cost, availability, and practicality.
Solar Powered Microplasma-Generated Ozone: Assessment of a Novel Point-of-Use Drinking Water Treatment Method
Authors: Dorevitch Samuel , Anderson Kendall , Shrestha Abhilasha , Wright Dorothy , Odhiambo Aloyce , Oremo Jared , Heimler Ira
Published: March 2020

Ozonation is widely used in high-income countries for water disinfection in centralized treatment facilities. New microplasma technology has reduced the energy requirements for ozone generation dramatically, such that a 15-watt solar panel is sufficient to produce small quantities of ozone. This technology has not been used previously for point-of-use drinking water treatment. We conducted a series of assessments of this technology, both in the laboratory and in homes of residents of a village in western Kenya, to estimate system efficacy and to determine if the solar-powered point-of-use water ozonation system appears safe and acceptable to end-users. In the laboratory, two hours of point-of-use ozonation reduced E. coli in 120 L of wastewater by a mean (standard deviation) of 2.3 (0.84) log-orders of magnitude and F+ coliphage by 1.54 (0.72). Based on laboratory efficacy, 10 families in Western Kenya used the system to treat 20 L of household stored water for two hours on a daily basis for eight weeks. Household stored water E. coli concentrations of >1000 most probable number (MPN)/100 mL were reduced by 1.56 (0.96) log removal value (LRV). No participants experienced symptoms of respiratory or mucous membrane irritation. Focus group research indicated that families who used the system for eight weeks had very favorable perceptions of the system, in part because it allowed them to charge mobile phones. Drinking water ozonation using microplasma technology may be a sustainable point-of-use treatment method, although system optimization and evaluations in other settings would be needed.
A “Forbidden Fruit Effect”: An Eye-Tracking Study on Children’s Visual Attention to Food Marketing
Authors: Binder Alice , Naderer Brigitte , Matthes Jörg
Published: March 2020

Obesity in children is an international health concern. Against this background, there is an increasing interest in understanding how healthy and unhealthy food marketing in narrative media can affect children. In particular, children’s implicit reactions, such as visual attention and emotional arousal, are far from being sufficiently understood. We conducted an eye-tracking study, presenting children one of two versions of a narrative media-stimulus, either presenting an unhealthy food (i.e., candy condition; N = 34), or a healthy food (i.e., fruit condition; N = 34). As dependent variables, we investigated dwell time (i.e., visual attention) and pupil dilation (i.e., emotional arousal). As moderators, we included children’s prohibition of candy at home and children’s level of BMI in our models. Our results indicate that mean dwell time did not differ between conditions and that the moderators did not exert any effect. Moreover, pupil dilation did not differ between conditions but was moderated by parents’ candy prohibition at home (ηp2 = 0.080). The results show that children who are not allowed to consume candy at home react with higher emotional arousal when exposed to candy placements than children allowed to eat candy at home. Thus, depending on children’s contextual factors, children react differently to unhealthy food cues.
Cancer Mortality and Deprivation in the Proximity of Polluting Industrial Facilities in an Industrial Region of Spain
Authors: Santos-Sánchez Vanessa , Córdoba-Doña Juan Antonio , García-Pérez Javier , Escolar-Pujolar Antonio , Pozzi Lucia , Ramis Rebeca
Published: March 2020

Residential proximity to industrial facilities that release pollutants is a source of exposure to a high number of toxics, many of them known or suspected carcinogens. The objective of the study was to analyze the association between lung, larynx, bladder, and kidney cancer mortality and deprivation in areas proximate to polluting industrial facilities in Cadiz, a highly industrialized province in Spain. An ecological study at census tract level was carried out to estimate the mortality rates associated with deprivation and proximity to polluting industrial facilities (1–5 km) using the Besag–York–Mollié model. The results show a negative social gradient for lung and larynx cancers in males and greater risk of lung cancer was observed in the least deprived areas in females. These associations were found regardless the distance to industrial facilities. Increasing excess risk (relative risk; 95% credibility interval) of lung cancer for males (1.09; 1.02–1.16 at 5 km vs 1.24; 1.08–1.41 at 1 km) and bladder cancer for males (1.11; 1.01–1.22 at 5 km vs 1.32; 1.08–1.60 at 1 km) and females (1.32; 1.04–1.69 at 4 km vs 1.91; 1.28–2.86 at 1 km) was found as proximity to polluting industrial facilities increased. For kidney cancer, high risks were observed near such facilities for both sexes. Knowing the possible influence of industrial pollution and social inequalities over cancer risk allows the definition of policies aimed at reducing the risk.
Sense of Coherence in Nurses: A Systematic Review
Authors: Michele Masanotti Giuseppe , Paolucci Silvia , Abbafati Elia , Serratore Claudio , Caricato Michela
Published: March 2020

Background: Nurses experience high levels of distress due to the nature of their work and workplaces; Antonovsky’s salutogenic theory shows that individual and work-related factors can influence human health. The aim of this paper is to analyze the possible correlations with different work-related and individual variables, which influence or are influenced by Sense of Coherence (SOC) and verify the possible use of SOC scales to prevent negative health determinants in workplaces. Methods: Electronic databases were searched with selected studies compared for sample, sample size, study design and basic results. Cross-sectional studies were reviewed for correlations between individual physical and mental health, distress, burnout, job satisfaction and SOC, with intervention studies used to assess the possible impact of training on nurses’ SOC. Results: The review found several correlations between SOC and different work-related variables; but also with several individual characteristics. Conclusion: The review found that SOC was predictor of depressive state, burnout, job dissatisfaction among female nurses; therefore, SOC could be a health promoting resource.
How Physical Activity across the Lifespan Can Reduce the Impact of Bone Ageing: A Literature Review
Authors: Faienza Maria Felicia , Lassandro Giuseppe , Chiarito Mariangela , Valente Federica , Ciaccia Loredana , Giordano Paola
Published: March 2020

Bone remodeling is a lifelong process, due to the balanced activity of the osteoblasts (OBs), the bone-forming cells, and osteoclasts (OCs), the bone-resorbing cells. This equilibrium is mainly regulated by the WNT-ß-cathenin pathway and the RANK-RANKL/OPG system, respectively. Bone ageing is a process which normally occurs during life due to the imbalance between bone formation and bone resorption, potentially leading to osteoporosis. Bone loss associated with bone ageing is determined by oxidative stress, the result of the increasing production of reactive oxygen species (ROS). The promotion of physical exercise during growth increases the chances of accruing bone and delaying the onset of osteoporosis. Several studies demonstrate that physical exercise is associated with higher bone mineral density and lower fracture incidence, and the resulting bone mineral gain is maintained with ageing, despite a reduction of physical activity in adulthood. The benefits of exercise are widely recognized, thus physical activity is considered the best non-pharmacologic treatment for pathologies such as osteoporosis, obesity, diabetes and cardiovascular disease. We reviewed the physiological mechanisms which control bone remodeling, the effects of physical activity on bone health, and studies on the impact of exercise in reducing bone ageing.
Evaluation of IAQ Management Using an IoT-Based Indoor Garden
Authors: Kim Ho-Hyun , Kwak Min-Jung , Kim Kwang-Jin , Gwak Yoon-Kyung , Lee Jeong-Hun , Yang Ho-Hyeong
Published: March 2020

This study was designed to verify the effectiveness of smart gardens by improving indoor air quality (IAQ) through the installation of an indoor garden with sensor-based Internet-of-Things (IoT) technology that identifies pollutants such as particulate matter. In addition, the study aims to introduce indoor gardens for customized indoor air cleaning using the data and IoT technology. New apartments completed in 2016 were selected and divided into four households with indoor gardens installed and four households without indoor gardens. Real-time data and data on PM2.5, CO2, temperature, and humidity were collected through an IoT-based IAQ monitoring system. In addition, in order to examine the effects on the health of occupants, the results were analyzed based on epidemiological data, prevalence data, current maintenance, and recommendation criteria, and were presented and evaluated as indices. The indices were classified into a comfort index, which reflects the temperature and humidity, an IAQ index, which reflects PM2.5 and CO2, and an IAQ composite index. The IAQ index was divided into five grades from “good” to “hazardous”. Using a scale of 1 to 100 points, it was determined as follows: “good (0–20)”, “moderate (21–40)”, “unhealthy for sensitive group (41–60)”, “bad (61–80)”, “hazardous (81–100)”. It showed an increase in the “good” section after installing the indoor garden, and the “bad” section decreased. Additionally, the comfort index was classified into five grades from “very comfortable” to “very uncomfortable”. In the comfort index, the “uncomfortable” section decreased, and the “comfortable” section increased after the indoor garden was installed.
Maternal Health and Nutrition Status, Human Milk Composition, and Growth and Development of Infants and Children: A Prospective Japanese Human Milk Study Protocol
Authors: Nojiri Keisuke , Kobayashi Shunjiro , Higurashi Satoshi , Takahashi Tomoki , Tsujimori Yuta , Ueno Hiroshi M. , Watanabe-Matsuhashi Shiomi , Toba Yasuhiro , Yamamura Junichi , Nakano Taku , Nomura Kyoko , Kobayashi Toshiya
Published: March 2020

It is unknown whether maternal health and nutrition are related to human milk composition or growth and development of infants and children. Here, we describe a protocol for a prospective five-year cohort study to clarify (i) how maternal health and nutrition, socioeconomic factors, and lifestyles affect human milk composition, and (ii) whether these are associated with growth and development of infants and children. In our study, we recruited 1210 Japanese mothers with singleton pregnancies from 73 obstetrics clinics and hospitals across Japan, between 2014 and 2019. We will measure the following: health information regarding maternal-child dyads using a self-administered questionnaire, maternal nutrition during breastfeeding using a Brief self-administrated Diet History Questionnaire, the development of infants and children using the Kinder Infant Development Scale, and the stress related to child rearing using the Mother’s Child Care Stress Scale. Simultaneously, we will collect human milk every 2 months during the first year after birth to measure its composition and levels of macronutrients. This study will generate useful data to investigate whether health status, nutritional status, lifestyle, and socioeconomic factors affect human milk composition and the growth and development of infants and children.
Demographics and Health Behavior of Video Game and eSports Players in Germany: The eSports Study 2019
Authors: Rudolf Kevin , Bickmann Peter , Froböse Ingo , Tholl Chuck , Wechsler Konstantin , Grieben Christopher
Published: March 2020

The number of video game and eSports players is steadily rising. Since little is known about their health behavior to date, the present study examines the demographics and health behavior of video game and eSports players. In this cross-sectional study, data on demographics, health status, physical activity, nutrition, sleep, and video game usage were assessed via a web-based survey of n = 1066 players (91.9% male; 22.9 ± 5.9 years; body mass index (BMI): 24.6 ± 4.8 kg/m²) in Germany in 2018. The majority of respondents (95%) reported a good to excellent health status. Two thirds (66.9%) engaged in moderate to vigorous physical activity for more than 2.5 h/week. The average duration of sitting and sleep time was 7.7 ± 3.6 h/day and 7.1 ± 1.3 h/day, respectively. Mean fruit and vegetable consumption was 2.7 ± 1.8 portions/day. Video games were played for 24.4 ± 15.9 h/week on average. Partial Spearman correlations revealed poor positive associations of video game play time to sedentary behavior (rho = 0.15; p < 0.01) and BMI (rho = 0.11; p < 0.01), as well as a poor negative association to self-reported health status (rho = −0.14; p < 0.01). These results indicate the good subjective health of this target group. Nevertheless, the high amount of video game play time and its poor negative association to health status indicate a need for specific health promotion strategies for this target group.
Later-Life Exposure to Moderate PM2.5 Air Pollution and Life Loss of Older Adults in Taiwan
Authors: Hwang Jing-Shiang , Hu Tsuey-Hwa
Published: March 2020

Background: Few studies have directly estimated expected life loss attributable to lifetime exposure to fine particulate matter (PM2.5). Methods: We used claims data from Taiwan’s National Health Insurance to create 63 study cohorts of 1.91 million residents aged 60–79 years old residing in small areas where air quality monitoring stations are situated. The survival status of each person was followed from 2001 to 2016. We applied an extrapolation algorithm to estimate the lifetime survival function so that we could directly estimate life expectancy (LE) and the lifetime exposure to PM2.5 of each cohort. We estimated the association between LE and lifetime exposure to PM2.5 among the 63 cohorts. We also fit a Cox proportional hazards model to all the data combined to estimate the relative risk of mortality. Results: Older adults who lived in an area with a higher lifetime weighted average PM2.5 of 10 μ g / m 3 had a shortened LE by 0.34 (95% CI: 0.22–0.46) years. The hazard ratio of mortality was 1.0245 (1.0242–1.0248) per one μ g / m 3 increase in lifetime average PM2.5. Conclusion: This study provides strong evidence that later-life exposure to moderate PM2.5 air pollution had a substantial impact on the life loss of older adults.
Even Warriors Can be Scared: A Survey Assessing Anxiety and Coping Skills in Competitive CrossFit Athletes
Authors: Wilke Jan , Pfarr Tatjana , Möller Mandy-Deborah
Published: March 2020

Competition anxiety has been demonstrated to decrease sports performance while increasing burnout risk. To date, its degree in CrossFit (CF) is unknown. The present study, therefore, examines competition fear and relevant coping skills as well as potential correlates of both in individuals participating in CF events. A total of n = 79 athletes answered a battery of three questionnaires (competition fear index, athletic coping skills inventory, mindfulness attention awareness scale). Substantial levels of anxiety, particularly regarding the somatic dimension of the competition fear index, were reported. The most pronounced coping skill was freedom of worry. While age or level of competition showed no/very small associations with survey data, sex was correlated to the psychological characteristics: women reported higher competition fears and lower coping skill levels (p > 0.05). Competition fears are highly prevalent in CF athletes and the preventive value of population-specific interventions, particularly in females, should be investigated in future trials.
Humanization of Tumor Stroma by Tissue Engineering as a Tool to Improve Squamous Cell Carcinoma Xenograft
Authors: Guerrero-Aspizua Sara , González-Masa Andrea , Conti Claudio J. , García Marta , Chacón-Solano Esteban , Larcher Fernando , del Río Marcela
Published: March 2020

The role of stroma is fundamental in the development and behavior of epithelial tumors. In this regard, limited growth of squamous cell carcinomas (SCC) or cell-lines derived from them has been achieved in immunodeficient mice. Moreover, lack of faithful recapitulation of the original human neoplasia complexity is often observed in xenografted tumors. Here, we used tissue engineering techniques to recreate a humanized tumor stroma for SCCs grafted in host mice, by combining CAF (cancer associated fibroblasts)-like cells with a biocompatible scaffold. The stroma was either co-injected with epithelial cell lines derived from aggressive SCC or implanted 15 days before the injection of the tumoral cells, to allow its vascularization and maturation. None of the mice injected with the cell lines without stroma were able to develop a SCC. In contrast, tumors were able to grow when SCC cells were injected into previously established humanized stroma. Histologically, all of the regenerated tumors were moderately differentiated SCC with a well-developed stroma, resembling that found in the original human neoplasm. Persistence of human stromal cells was also confirmed by immunohistochemistry. In summary, we provide a proof of concept that humanized tumor stroma, generated by tissue engineering, can facilitate the development of epithelial tumors in immunodeficient mice.
The Protective Effect of Dabigatran and Rivaroxaban on DNA Oxidative Changes in a Model of Vascular Endothelial Damage with Oxidized Cholesterol
Authors: Woźniak Ewelina , Broncel Marlena , Bukowska Bożena , Gorzelak-Pabiś Paulina
Published: March 2020

Background: Atherosclerotic plaques are unstable, and their release may result in thrombosis; therefore, currently, antiplatelet therapy with anticoagulants is recommended for the treatment of acute coronary syndrome. The aim of this study was to assess the effect of oxidized cholesterol on human umbilical vascular endothelial cells (HUVECs). The study also examines the protective and repairing effect of dabigatran and rivaroxaban in a model of vascular endothelial damage with 25-hydroxycholesterol (25-OHC). Methods: HUVECs were treated with compounds induce DNA single-strand breaks (SSBs) using the comet assay. Oxidative DNA damage was detected using endonuclease III (Nth) or human 8 oxoguanine DNA glycosylase (hOOG1). Reactive oxygen species (ROS) formation was determined using flow cytometry. Results: 25-hydroxycholesterol caused DNA SSBs, induced oxidative damage and increased ROS in the HUVECs; ROS level was lowered by dabigatran and rivaroxaban. Only dabigatran was able to completely repair the DNA SSBs induced by oxysterol. Dabigatran was able to reduce the level of oxidative damage of pyrimidines induced by oxysterol to the level of control cells. Conclusions: Observed changes strongly suggest that the tested anticoagulants induced indirect repair of DNA by inhibiting ROS production. Furthermore, dabigatran appears to have a higher antioxidant activity than rivaroxaban.
Role of Neutrophils and Myeloid-Derived Suppressor Cells in Glioma Progression and Treatment Resistance
Authors: Khan Sabbir , Mittal Sandeep , McGee Kain , Alfaro-Munoz Kristin D. , Majd Nazanin , Balasubramaniyan Veerakumar , de Groot John F.
Published: March 2020

Recent efforts in brain tumor research have been directed towards the modulation of the immune system for therapeutic interventions. Several human cancers, including gliomas, are infiltrated with immune cell types—including neutrophils and myeloid-derived suppressor cells—that contribute to tumor progression, invasiveness, and treatment resistance. The role of tumor-associated neutrophils and myeloid-derived suppressor cells in cancer biology remains elusive, as these cells can exert a multitude of pro-tumor and antitumor effects. In this review, we provide the current understanding and novel insights on the role of neutrophils and myeloid-derived suppressor cells in glioma progression and treatment resistance, as well as the mechanisms of pleiotropic behaviors in these cells during disease progression, with an emphasis on possible strategies to reprogram these cells towards their antitumor actions.
Animal Model for Prodromal Parkinson’s Disease
Authors: Taguchi Tomoyuki , Ikuno Masashi , Yamakado Hodaka , Takahashi Ryosuke
Published: March 2020

Parkinson’s disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and subsequent motor symptoms, but various non-motor symptoms (NMS) often precede motor symptoms. Recently, NMS have attracted much attention as a clue for identifying patients in a prodromal stage of PD, which is an excellent point at which to administer disease-modifying therapies (DMTs). These prodromal symptoms include olfactory loss, constipation, and sleep disorders, especially rapid eye movement sleep behavior disorder (RBD), all of which are also important for elucidating the mechanisms of the initiation and progression of the disease. For the development of DMTs, an animal model that reproduces the prodromal stage of PD is also needed. There have been various mammalian models reported, including toxin-based, genetic, and alpha synuclein propagation models. In this article, we review the animal models that exhibit NMS as prodromal symptoms and also discuss an appropriate prodromal model and its importance for the development of DMT of PD.
Tuning the Polymorphism of the Anti-VEGF G-rich V7t1 Aptamer by Covalent Dimeric Constructs
Authors: Riccardi Claudia , Musumeci Domenica , Platella Chiara , Gaglione Rosa , Arciello Angela , Montesarchio Daniela
Published: March 2020

In the optimization process of nucleic acid aptamers, increased affinity and/or activity are generally searched by exploring structural analogues of the lead compound. In many cases, promising results have been obtained by dimerization of the starting aptamer. Here we studied a focused set of covalent dimers of the G-quadruplex (G4) forming anti-Vascular Endothelial Growth Factor (VEGF) V7t1 aptamer with the aim of identifying derivatives with improved properties. In the design of these covalent dimers, connecting linkers of different chemical nature, maintaining the same polarity along the strand or inverting it, have been introduced. These dimeric aptamers have been investigated using several biophysical techniques to disclose the conformational behavior, molecularity and thermal stability of the structures formed in different buffers. This in-depth biophysical characterization revealed the formation of stable G4 structures, however in some cases accompanied by alternative tridimensional arrangements. When tested for their VEGF165 binding and antiproliferative activity in comparison with V7t1, these covalent dimers showed slightly lower binding ability to the target protein but similar if not slightly higher antiproliferative activity on human breast adenocarcinoma MCF-7 cells. These results provide useful information for the design of improved dimeric aptamers based on further optimization of the linker joining the two consecutive V7t1 sequences.
Trifunctionalized Naphthalene Diimides and Dimeric Analogues as G-Quadruplex-Targeting Anticancer Agents Selected by Affinity Chromatography
Authors: Platella Chiara , Pirota Valentina , Musumeci Domenica , Rizzi Federica , Iachettini Sara , Zizza Pasquale , Biroccio Annamaria , Freccero Mauro , Montesarchio Daniela , Doria Filippo
Published: March 2020

A focused library of newly designed monomeric and dimeric naphthalene diimides (NDIs) was analyzed in its ability to recognize specific G-quadruplex (G4) structures discriminating duplex DNA. The best G4 ligands—according to an affinity chromatography-based screening method named G4-CPG—were tested on human cancer and healthy cells, inducing DNA damage at telomeres, and in parallel, showing selective antiproliferative activity on HeLa cancer cells with IC50 values in the low nanomolar range. CD and fluorescence spectroscopy studies allowed detailed investigation of the interaction in solution with different G4 and duplex DNA models of the most promising NDI of the series, as determined by combining the biophysical and biological assays’ data.
Ganglioside GM3 Up-Regulate Chondrogenic Differentiation by Transform Growth Factor Receptors
Authors: Ryu Jae-Sung , Seo Sang Young , Jeong Eun-Jeong , Kim Jong-Yeup , Koh Yong-Gon , Kim Yong Il , Choo Young-Kug
Published: March 2020

Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage. Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules. These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition. Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation. However, the role of gangliosides in chondrogenesis is not understood. In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells. We showed that the gangliosides GM3 and GD3 were expressed after the chondrogenic differentiation of hSMSC aggregates. To test whether GM3 affected the chondrogenic differentiation of hSMSC aggregates, we used GM3 treatment during chondrogenic differentiation. The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group. Furthermore, GM3 treatment enhanced TGF-β signaling via SMAD 2/3 during the chondrogenic differentiation of hSMSC aggregates. Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
Actin Depolymerizing Factor Modulates Rhizobial Infection and Nodule Organogenesis in Common Bean
Authors: Ortega-Ortega Yolanda , Carrasco-Castilla Janet , Juárez-Verdayes Marco A. , Toscano-Morales Roberto , Fonseca-García Citlali , Nava Noreide , Cárdenas Luis , Quinto Carmen
Published: March 2020

Actin plays a critical role in the rhizobium–legume symbiosis. Cytoskeletal rearrangements and changes in actin occur in response to Nod factors secreted by rhizobia during symbiotic interactions with legumes. These cytoskeletal rearrangements are mediated by diverse actin-binding proteins, such as actin depolymerization factors (ADFs). We examined the function of an ADF in the Phaseolus vulgaris–rhizobia symbiotic interaction (PvADFE). PvADFE was preferentially expressed in rhizobia-inoculated roots and nodules. PvADFE promoter activity was associated with root hairs harbouring growing infection threads, cortical cell divisions beneath root hairs, and vascular bundles in mature nodules. Silencing of PvADFE using RNA interference increased the number of infection threads in the transgenic roots, resulting in increased nodule number, nitrogen fixation activity, and average nodule diameter. Conversely, overexpression of PvADFE reduced the nodule number, nitrogen fixation activity, average nodule diameter, as well as NODULE INCEPTION (NIN) and EARLY NODULIN2 (ENOD2) transcript accumulation. Hence, changes in ADFE transcript levels affect rhizobial infection and nodulation, suggesting that ADFE is fine-tuning these processes.
Toward Effective Medical Image Analysis Using Hybrid Approaches—Review, Challenges and Applications
Authors: Bourouis Sami , Alroobaea Roobaea , Rubaiee Saeed , Ahmed Anas
Published: March 2020

Accurate medical images analysis plays a vital role for several clinical applications. Nevertheless, the immense and complex data volume to be processed make difficult the design of effective algorithms. The first aim of this paper is to examine this area of research and to provide some relevant reference sources related to the context of medical image analysis. Then, an effective hybrid solution to further improve the expected results is proposed here. It allows to consider the benefits of the cooperation of different complementary approaches such as statistical-based, variational-based and atlas-based techniques and to reduce their drawbacks. In particular, a pipeline framework that involves different steps such as a preprocessing step, a classification step and a refinement step with variational-based method is developed to identify accurately pathological regions in biomedical images. The preprocessing step has the role to remove noise and improve the quality of the images. Then the classification is based on both symmetry axis detection step and non linear learning with SVM algorithm. Finally, a level set-based model is performed to refine the boundary detection of the region of interest. In this work we will show that an accurate initialization step could enhance final performances. Some obtained results are exposed which are related to the challenging application of brain tumor segmentation.
Voice Communication Systems Impact on Navigating Officers
Authors: Maglić Lovro , Valčić Sanjin , Gundić Ana , Maglić Livia
Published: March 2020

The tasks of an officer of the watch (OOW) on complex ships during navigation in coastal areas may be very challenging. Almost all the tasks require substantial information processing and timely decision making. Every distracting element should be avoided during navigation. Every call, made through any communication system, activates a new process that the OOW needs to conduct. The new process may interrupt a previously started task. In case of too many incoming calls, the workload of the OOW may increase significantly, thus, their situational awareness may be compromised and the risk of errors may increase. The objective of this research was to analyze the impact of incoming voice calls on the OOW. The research methods used include a questionnaire survey and a series of interviews with experienced officers. The main outcomes refer to the average frequency of incoming calls, duration of conversations and subjective assessment of their influence. The results indicate that, during one watch, an officer needs to answer 14 calls that last 16.19 min altogether. However, the officers consider 45% of calls made during watch keeping as distracting. A possible call management system with the aim to reduce distractions made by low priority calls is proposed in this paper.
Atmospheric Pressure Plasma Deposition of Organosilicon Thin Films by Direct Current and Radio-frequency Plasma Jets
Authors: Kuchakova Iryna , Ionita Maria Daniela , Ionita Eusebiu-Rosini , Lazea-Stoyanova Andrada , Brajnicov Simona , Mitu Bogdana , Dinescu Gheorghe , De Vrieze Mike , Cvelbar Uroš , Zille Andrea , Leys Christophe , Yu Nikiforov Anton
Published: March 2020

Thin film deposition with atmospheric pressure plasmas is highly interesting for industrial demands and scientific interests in the field of biomaterials. However, the engineering of high-quality films by high-pressure plasmas with precise control over morphology and surface chemistry still poses a challenge. The two types of atmospheric-pressure plasma depositions of organosilicon films by the direct and indirect injection of hexamethyldisiloxane (HMDSO) precursor into a plasma region were chosen and compared in terms of the films chemical composition and morphology to address this. Although different methods of plasma excitation were used, the deposition of inorganic films with above 98% of SiO2 content was achieved for both cases. The chemical structure of the films was insignificantly dependent on the substrate type. The deposition in the afterglow of the DC discharge resulted in a soft film with high roughness, whereas RF plasma deposition led to a smoother film. In the case of the RF plasma deposition on polymeric materials resulted in films with delamination and cracks formation. Lastly, despite some material limitations, both deposition methods demonstrated significant potential for SiOx thin-films preparation for a variety of bio-related substrates, including glass, ceramics, metals, and polymers.
Production of Gamma Alumina Using Plasma-Treated Aluminum and Water Reaction Byproducts
Authors: Urbonavicius Marius , Varnagiris Sarunas , Pranevicius Liudas , Milcius Darius
Published: March 2020

High purity hydrogen and solid-state byproducts are produced using a proposed plasma-activated aluminum and water reactions approach. These byproducts could be transformed into pure gamma Al2O3 powder material, while hydrogen can be used for electricity generation. Various chemical methods can be used for the synthesis of gamma alumina, but most could result in high levels of remaining impurities. Boehmite is a cost-effective starting material for the production of high-purity Al2O3. Herein, we present a novel method for the synthesis of boehmite and its transformation into high-specific-surface-area γ-alumina. Specifically, this method implicates the direct reaction between distilled water and plasma-treated aluminum powder. The results show the structural and morphological changes of the byproduct of the aluminum/water reaction to boehmite and γ-Al2O3 after a simple heating procedure (at 280 and 500 °C respectively). The high-purity hydrogen produced during the aluminum/water reaction can be used for the high-efficiency and environmentally friendly production of electrical energy.
Effect of Ni Doping Content on Phase Transition and Electrochemical Performance of TiO2 Nanofibers Prepared by Electrospinning Applied for Lithium-Ion Battery Anodes
Authors: Kang Danning , Li Jun , Zhang Yuyao
Published: March 2020

Titanium dioxide (TiO2), as a potential anode material applied for lithium-ion batteries (LIBs), is constrained due to its poor theoretical specific capacity (335 mAh·g−1) and low conductivity (10−7-10−9 S·cm−1). When compared to TiO2, NiO with a higher theoretical specific capacity (718 mAh·g−1) is regarded as an alternative dopant for improving the specific capacity of TiO2. The present investigations usually assemble TiO2 and NiO with a simple bilayer structure and without NiO that is immersed into the inner of TiO2, which cannot fully take advantage of NiO. Therefore, a new strategy was put forward to utilize the synergistic effect of TiO2 and NiO, namely doping NiO into the inner of TiO2. NiO-TiO2 was fabricated into the nanofibers with a higher specific surface area to further improve their electrochemical performance due to the transportation path being greatly shortened. NiO-TiO2 nanofibers are expected to replace of the commercialized anode material (graphite). In this work, a facile one-step electrospinning method, followed by annealing, was applied to synthesize the Ni-doped TiO2 nanofibers. The Ni doping content was proven to be a crucial factor affecting phase constituents, which further determined the electrochemical performance. When the Ni doping content was less than 3 wt.%, the contents of anatase and NiO were both increased, while the rutile content was decreased in the nanofibers. When the Ni doping content exceeded 3 wt.%, the opposite changes were observed. Hence, the optimum Ni doping content was determined as 3 wt.%, at which the highest weight fractions of anatase and NiO were obtained. Correspondingly, the obtained electronic conductivity of 4.92 × 10−5 S⋅cm−1 was also the highest, which was approximately 1.7 times that of pristine TiO2. The optimal electrochemical performance was also obtained. The initial discharge and charge specific capacity was 576 and 264 mAh·g−1 at a current density of 100 mA·g−1. The capacity retention reached 48% after 100 cycles, and the coulombic efficiency was about 100%. The average discharge specific capacity was 48 mAh·g−1 at a current density of 1000 mA·g−1. Approximately 65.8% of the initial discharge specific capacity was retained when the current density was recovered to 40 mA·g−1. These excellent electrochemical results revealed that Ni-doped TiO2 nanofibers could be considered to be promising anode materials for LIBs.
Effect of Relative Humidity on Mechanical Degradation of Medium Mn Steels
Authors: Liu Qingyang , Xu Juanping , Shen Liancheng , Zhou Qingjun , Su Yanjing , Qiao Lijie , Yan Yu
Published: March 2020

Medium Mn steels have been considered as the next-generation materials for use in the automotive industry due to their excellent strength and ductility balance. To reduce the total weight and improve the safety of vehicles, medium Mn steels look forward to a highly promising future. However, hydrogen-induced delayed cracking is a concern for the use of high strength steels. This work is focused on the service characteristics of two kinds of medium Mn steels under different relative humidity conditions (40%, 60%, 80% and 100%). Under normal relative humidity (about 40%) at 25 °C, the hydrogen concentration in steel is 0.4 ppm. When exposed to higher relative humidity, the hydrogen concentration in steel increases slowly and reaches a stable value, about 0.8 ppm. In slow strain rate tensile tests under different relative humidity conditions, the tensile strength changed, the hydrogen concentration increased and the elongation decreased as well, thereby increasing the hydrogen embrittlement sensitivity. In other words, the smaller the tensile rate applied, the greater the hydrogen embrittlement sensitivity. In constant load tests under different relative humidity conditions, the threshold value of the delayed cracking of M7B (‘M’ referring to Mn, ‘7’ meaning the content of Mn, ‘B’ denoting batch annealing) steel maintains a steady value of 0.82 σb (tensile strength). The threshold value of the delayed cracking of M10B significantly changed along with relative humidity. When relative humidity increased from 60% to 80%, the threshold dropped sharply from 0.63 σb to 0.52 σb. We define 80% relative humidity as the ‘threshold humidity’ for M10B.