bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-core food product advertising on free-to-air television in Hong Kong
Authors: Cheung Vivien Hui In , Louie Jimmy Chun Yu
Published: June 2020

Objective:

To study the extent and nature of free-to-air television advertisements for non-core products (e.g., fast food or soda) directed at children in Hong Kong.

Design:

Television programs from two major Hong Kong free-to-air television channels airing between 06.00 and 24.00 hours from October 2018 to January 2019 were recorded. Eight nonconsecutive days (four weekdays and four weekend days) were selected for analysis. Pearson’s χ2 tests were conducted to compare the pattern of food advertisements by program categories, days of the week, television viewing periods and persuasive marketing techniques.

Setting:

Free-to-air television programs.

Participants:

Not applicable.

Results:

Of the 10 348 commercials identified, 18·4 % were for foods, and 35·2 % of these were for non-core items. Baby and toddler milk formula (19·5 %) were the most advertised food products, while the most frequently advertised non-core food was fast foods (12·3 %). There was a higher non-core to core product ratio during prime time than the children’s time slot (7 v. 1·7). Non-sports celebrity endorsement (27·1 %) was the most frequently used persuasive marketing technique overall, while that for non-core products was sensory characteristics (38·2 %). Most food product placements recorded were non-core products, mentions of local and fast food restaurants and recipe additions.

Conclusions:

Non-core products were highly advertised in Hong Kong, while core product advertising was infrequent. Regulations on junk food advertising in Hong Kong should focus on prime time, as well as on food product placement, to reduce children’s exposure to persuasive junk food marketing.
Population-level effective coverage of adolescent weekly iron and folic acid supplementation is low in rural West Bengal, India
Authors: Sudfeld Christopher R , Rai Rajesh Kumar , Barik Anamitra , Valadez Joseph J , Fawzi Wafaie W
Published: June 2020

Objective:

To assess the coverage of the adolescent weekly iron and folic acid supplementation (WIFS) programme in rural West Bengal, India.

Design:

We conducted a population-based cross-sectional survey of intended WIFS programme beneficiaries (in-school adolescent girls and boys and out-of-school adolescent girls).

Setting:

Birbhum Health and Demographic Surveillance System.

Participants:

A total of 4448 adolescents 10–19 years of age participated in the study.

Results:

The percentage of adolescents who reported taking four WIFS tablets during the last month as intended by the national programme was 9·4 % among in-school girls, 7·1 % for in-school boys and 2·3 % for out-of-school girls. The low effective coverage was due to the combination of large deficits in WIFS provision and poor adherence. A large proportion of adolescents reported they were not provided any WIFS tablets in the last month: 61·7 % of in-school girls, 73·3 % of in-school boys and 97·1 % of out-of-school girls. In terms of adherence, only 41·6 % of in-school girls, 38·1 % of in-school boys and 47·4 % of out-of-school girls reported that they consumed all WIFS tablets they received. Counselling from teachers, administrators and school staff was the primary reason adolescents reported taking WIFS tablets, whereas the major reasons for non-adherence were lack of perceived benefit, peer suggestion not to take WIFS and a reported history of side effects.

Conclusions:

The effective coverage of the WIFS programme for in-school adolescents and out-of-school adolescent girls is low in rural Birbhum. Integrated supply- and demand-side strategies appear to be necessary to increase the effective coverage and potential benefits of the WIFS programme.
Invigorating Hydrological Research Through Journal Publications
Authors: Quinn Nevil , Blöschl Günter , Bárdossy András , Castellarin Attilio , Clark Martyn , Cudennec Christophe , Koutsoyiannis Demetris , Lall Upmanu , Lichner Lubomir , Parajka Juraj , Peters‐Lidard Christa D. , Sander Graham , Savenije Hubert , Smettem Keith , Vereecken Harry , Viglione Alberto , Willems Patrick , Wood Andy , Woods Ross , Xu Chong‐Yu , Zehe Erwin
Published: June 2020

Editors of several journals in the field of hydrology met during the General Assembly of the European Geosciences Union—EGU in Vienna in April 2017. This event was a follow‐up of similar meetings held in 2013 and 2015. These meetings enable the group of editors to review the current status of the journals and the publication process and to share thoughts on future strategies. Journals were represented at the 2017 meeting by their editors, as shown in the list of authors. The main points on invigorating hydrological research through journal publications are communicated in this joint editorial published in the above journals.
Submarine Landslide Kinematics Derived From High‐Resolution Imaging in Port Valdez, Alaska
Authors: Roland Emily , Haeussler Peter , Parsons Tom , Hart Patrick
Published: June 2020

Submarine landslides caused by strong ground shaking during the M9.2 1964 Great Alaska earthquake generated a tsunami that destroyed much of the old town of Valdez, Alaska, and was responsible for 32 deaths at that location. We explore structural details of the 1964 landslide deposit, as well as landslide deposits from earlier events, in order to characterize kinematics of the landslide process. We present a new high‐resolution seismic reflection data set that images the 1964 landslide deposit and six pre‐1964 deposits with great detail. These deposits are represented by thick packages (~7–23 m) of debris within >500 m of fjord sedimentation above basement. Internal slide structures are associated with distinctive landslide failure mechanisms, including detailed erosional and depositional features and structures resolved within both landslide blocks and distal debris flow layers. Based on comparisons of deposit volume from subbottom structure and differenced bathymetry, we refine prior interpretations of the source of failed material. New data show evidence for basal erosion and reworking of fjord‐floor sedimentation. Additionally, material comprising the 1964 landslide appears to have been translated and deformed by lateral thrusting, rather than having been sourced entirely from upslope evacuation zones. Taking into account these complexities in depositional patterns, we show variations in slide size through Holocene time and relate the history of landslides to the paleoseismic record. Collectively, these new observations demonstrate that Port Valdez has a repeated history of large submarine landslides, which are likely associated with large megathrust earthquakes.
Land Use and Land Cover Change Strongly Modulates Land‐Atmosphere Coupling and Warm‐Season Precipitation Over the Central United States in CESM2‐VR
Authors: Devanand Anjana , Huang Maoyi , Lawrence David M. , Zarzycki Colin M. , Feng Zhe , Lawrence Peter J. , Qian Yun , Yang Zhao
Published: June 2020

Prior research indicates that land use and land cover change (LULCC) in the central United States has led to significant changes in surface climate. The spatial resolution of simulations is particularly relevant in this region due to its influence on model skill in capturing mesoscale convective systems (MCSs) and on representing the spatial heterogeneity. Recent advances in Earth system models (ESMs) make it feasible to use variable resolution (VR) meshes to study regional impacts of LULCC while avoiding inconsistencies introduced by lateral boundary conditions typically seen in limited area models. Here, we present numerical experiments using the Community Earth System Model version 2–VR to evaluate (1) the influence of resolution and land use on model skill and (2) impacts of LULCC over the central United States at different resolutions. These simulations are configured either on the 1° grid or a VR grid with grid refinement to 1/8° over the contiguous United States for the period of 1984–2010 with two alternative land use data sets corresponding to the preindustrial and present day states. Our results show that skill in simulating precipitation over the central United States is primarily dependent on resolution, whereas skill in simulating 2‐m temperature is more dependent on accurate land use. The VR experiments show stronger LULCC‐induced precipitation increases over the Midwest in May and June, corresponding to an increase in the number of MCS‐like features and a more conductive thermodynamic environment for convection. Our study demonstrates the potential of using VR ESMs for hydroclimatic simulations in regions with significant LULCC.
Forecasting GOES 15 >2 MeV Electron Fluxes From Solar Wind Data and Geomagnetic Indices
Authors: Forsyth C. , Watt C. E. J. , Mooney M. K. , Rae I. J. , Walton S. D. , Horne R. B.
Published: June 2020

The flux of > 2 MeV electrons at geosynchronous orbit is used by space weather forecasters as a key indicator of enhanced risk of damage to spacecraft in low, medium, or geosynchronous Earth orbits. We present a methodology that uses the amount of time a single input data set (solar wind data or geomagnetic indices) exceeds a given threshold to produce deterministic and probabilistic forecasts of the >2 MeV flux at GEO exceeding 1,000 or 10,000 cm−2 s−1 sr−1 within up to 10 days. By comparing our forecasts with measured fluxes from GOES 15 between 2014 and 2016, we determine the optimum forecast thresholds for deterministic and probabilistic forecasts by maximizing the receiver‐operating characteristic (ROC) and Brier skill scores, respectively. The training data set gives peak ROC scores of 0.71 to 0.87 and peak Brier skill scores of −0.03 to 0.32. Forecasts from AL give the highest skill scores for forecasts of up to 6 days. AL, solar wind pressure, or SYM‐H give the highest skill scores over 7–10 days. Hit rates range over 56–89% with false alarm rates of 11–53%. Applied to 2012, 2013, and 2017, our best forecasts have hit rates of 56–83% and false alarm rates of 10–20%. Further tuning of the forecasts may improve these. Our hit rates are comparable to those from operational fluence forecasts, that incorporate fluence measurements, but our false alarm rates are higher. This proof‐of‐concept shows that the geosynchronous electron flux can be forecast with a degree of success without incorporating a persistence element into the forecasts.
River Plume Liftoff Dynamics and Surface Expressions
Authors: Branch R. A. , Horner‐Devine A. R. , Kumar N. , Poggioli A. R.
Published: June 2020

The water surface expression of liftoff and its dependence on discharge are examined using numerical simulations with the Regional Ocean Modeling System (ROMS). Liftoff is the process by which buoyant river water separates from the bed and flows over denser saltwater. During low‐discharge conditions liftoff occurs in the river and is accompanied by a change in the surface slope. During high‐discharge conditions liftoff occurs outside the mouth and generates a ridge on the water surface. The location and height of the ridge can be described by analytical equations in terms of discharge, shelf slope, and river mouth aspect ratio. The offshore distance and height of the ridge are proportional to the river discharge and vary inversely with river mouth aspect ratio. For steep shelf slopes liftoff occurs close to the river mouth and generates a large ridge. The ridge is modified, but not eliminated, by the presence of tides. The water surface slope change at the ridge peak is large enough to be detected by the upcoming Surface Water and Ocean Topography (SWOT) altimeter and can be used to identify the liftoff location during high discharge. However, during low discharge the water surface slope change at the liftoff location is too small to be detected by SWOT. These results indicate that remote measurements of the presence or absence of the ridge may be useful to distinguish between low and high flows, and remote measurements of the ridge location or height could be used to estimate freshwater discharge.
Modeling the Role of Root Exudation in Critical Zone Nutrient Dynamics
Authors: Roque‐Malo Susana , Woo Dong Kook , Kumar Praveen
Published: June 2020

The multifaceted interface of plant roots, microbes, water, and soil can be considered a critical zone within the Critical Zone as it is host to many important dynamically linked processes, including the promotion of nutrient cycling through absorption and rhizodeposition, interaction and feedbacks with microorganisms and fungi, root‐facilitated hydraulic redistribution, and soil carbon dynamics. Such important processes in the Critical Zone have not been fully characterized and modeled in an ecohydrologic framework linking above‐ground natural and/or anthropogenic processes to below‐ground biogeochemical cycling. Specifically, the relation between root exudates and nutrient cycling remains an open challenge. Here we present the model REWT (Root Exudation in Watershed‐scale Transport) to demonstrate the systematic modeling of root exudation in an interconnected ecohydrologic framework. REWT incorporates an explicit dynamic root exudation transport model, nutrient absorption, and coupled microbial processes within the framework of a validated ecohydrologic model. Model simulations demonstrate the influence of root exudation of glucose, a polysaccharide that serves as fuel for microbes, and flavonoids, which can act as a biological nitrification inhibitor on microbial processes linked to soil carbon and nitrogen cycling. To demonstrate the capabilities of this theoretical framework, we parameterize REWT for corn and soybean crops in the Midwestern United States, and simulations indicate that rates of nitrification and respiration were substantially altered compared to model simulations in which root exudation was not explicitly included. This work demonstrates the importance of systematically incorporating root exudates into hydrobiogeochemical models and can serve to inform experimental design for active root zone processes.
Land Use Effects on Sediment Nutrient Processes in a Heavily Modified Watershed Using Structural Equation Models
Authors: Kreiling R. M. , Thoms M. C. , Bartsch L. A. , Larson J. H. , Christensen V. G.
Published: June 2020

Contemporary land use can affect sediment nutrient processes in rivers draining heavily modified watersheds; however, studies linking land use to sediment nutrient processes in large river networks are limited. In this study, we developed and evaluated structural equation models for denitrification and phosphorus retention capacity to determine direct and indirect linkages between current land use and sediment nutrient processes during base flow in the Fox River watershed, WI, USA. A large spatial‐scale dataset used for this study included sediment nitrogen and phosphorus retention measurements and land use information for 106 sites. The structural equation models for the Fox River watershed identified direct links between current land use and in‐stream sediment nutrient processes. Subwatersheds with agricultural land consisting of more natural land cover had lower surface water nitrate concentrations and higher denitrification enzyme activity than subwatersheds with less alternative cover. This indicates that best management practices implemented in the Fox River watershed that restore natural land cover can improve water quality through nitrogen removal on the agricultural landscape and in the river network. Best management practices are not having the same measurable effects on phosphorus in the river network, most likely due to legacy phosphorus stored in the sediment.
Atmospheric Temperature Effect in Secondary Cosmic Rays Observed With a 2 m2 Ground‐Based tRPC Detector
Authors: Riádigos Irma , García‐Castro Damián , González‐Díaz Diego , Pérez‐Muñuzuri Vicente
Published: June 2020

A high time resolution 2 m2 tracking detector, based on timing Resistive Plate Chamber (tRPC) cells, has been installed at the Faculty of Physics of the University of Santiago de Compostela (Spain) in order to improve our understanding of the cosmic rays arriving at the Earth's surface. Following a short commissioning of the detector, a study of the atmospheric temperature effect of the secondary cosmic ray component was carried out. To take into account this effect, temperature coefficients, WT(h), were obtained from cosmic ray data using a method based on Principal Component Analysis (PCA). The results obtained show good agreement with the theoretical expectation. The method successfully removes the correlation present between the different atmospheric layers, which would be dominant otherwise. We briefly describe the initial calibration and pressure correction procedures, essential to isolate the temperature effect. Overall, the measured cosmic ray rate displays the expected anticorrelation with the effective atmospheric temperature, through the coefficient αT=−0.279±0.051%/K. Rates follow the seasonal variations, and unusual short‐term events are clearly identified too.
Changes in West African Summer Monsoon Precipitation Under Stratospheric Aerosol Geoengineering
Authors: Da‐Allada C. Y. , Baloïtcha E. , Alamou E. A. , Awo F. M. , Bonou F. , Pomalegni Y. , Biao E. I. , Obada E. , Zandagba J. E. , Tilmes S. , Irvine P. J.
Published: June 2020

Stratospheric aerosol geoengineering (SAG) is suggested as a potential way to reduce the climate impacts of global warming. Using simulations from the Geoengineering Large Ensemble project that employed stratospheric sulfate aerosols injection to keep global mean surface temperature and also the interhemispheric and equator‐to‐pole temperature gradients at their 2020 values (present‐day climate) under Representative Concentration Pathway 8.5 scenario, we investigate the potential impact of SAG on the West African Summer Monsoon (WASM) precipitation and the involved physical processes. Results indicate that under Representative Concentration Pathway 8.5, during the monsoon period, precipitation increases by 44.76%, 19.74%, and 5.14% compared to the present‐day climate in the Northern Sahel, Southern Sahel, and Western Africa region, respectively. Under SAG, relative to the present‐day climate, the WASM rainfall is practically unchanged in the Northern Sahel region but in Southern Sahel and Western Africa regions, rainfall is reduced by 4.06% (0.19 ± 0.22 mm) and 10.87% (0.72 ± 0.27 mm), respectively. This suggests that SAG deployed to offset all warming would be effective at offsetting the effects of climate change on rainfall in the Sahel regions but that it would be overeffective in Western Africa, turning a modest positive trend into a negative trend twice as large. By applying the decomposition method, we quantified the relative contribution of different physical mechanisms responsible for precipitation changes under SAG. Results reveal that changes in the WASM precipitation are mainly driven by the reduction of the low‐level land‐sea thermal contrast that leads to weakened monsoon circulation and a northward shift of the monsoon precipitation.
South Atlantic Transect: Variations in Oceanic Crustal Structure at 31°S
Authors: Christeson G. L. , Reece R. S. , Kardell D. A. , Estep J. D. , Fedotova A. , Goff J. A.
Published: June 2020

We present an analysis of geophysical data acquired along a transect of 0–62 Ma crust located on the western flank of the Mid‐Atlantic Ridge at 31°S; all crust was formed at the same ridge segment. Crustal thickness, constrained by five wide‐angle profiles, has mean values of 5.6 km at 6.6 and 15.2 Ma, 7.0 km at 30.6 Ma, 5.5 km at 49.2 Ma, and 3.6 km at 61.2 Ma. Crustal thickness is uniform along each ridge‐parallel profile (standard deviations 0.1–0.3 km), indicating uniform along‐axis magmatic accretion over lateral distances of 40–60 km. The crustal structure of 61.2 Ma crust is not only anomalously thin compared to the other profiles but also contains regions with a linear velocity gradient from seafloor to Moho, which suggests that intense fracturing may extend to the base of the thin crust. Abyssal hill root‐mean‐square heights in the study region are 57–142 m and have an inverse correlation with spreading rate. These values are lower than the average root‐mean‐square height of 196 m elsewhere on the southern Mid‐Atlantic Ridge and indicate relatively high mantle temperatures in our study area. Unsedimented or lightly sedimented basement highs are prevalent at all ages; we argue that bottom currents scour the high topography, transporting sediment into adjacent basement lows. All drillsites planned for International Ocean Discovery Program Expeditions 390 and 393 are within 1–10 km of unsedimented or lightly sedimented basement highs, which should facilitate fluid flow and continued geochemical exchange between crust and seafloor.
Particle‐in‐Cell Experiments Examine Electron Diffusion by Whistler‐Mode Waves: 2. Quasi‐Linear and Nonlinear Dynamics
Authors: Allanson O. , Watt C. E. J. , Ratcliffe H. , Allison H. J. , Meredith N. P. , Bentley S. N. , Ross J. P. J. , Glauert S. A.
Published: June 2020

Test particle codes indicate that electron dynamics due to interactions with low amplitude incoherent whistler mode‐waves can be adequately described by quasi‐linear theory. However there is significant evidence indicating that higher amplitude waves cause electron dynamics not adequately described using quasi‐linear theory. Using the method that was introduced in Allanson et al. (2019, https://doi.org/10.1029/2019JA027088), we track the dynamical response of electrons due to interactions with incoherent whistler‐mode waves, across all energy and pitch angle space. We conduct five experiments each with different values of the electromagnetic wave amplitude. We find that the electron dynamics agree well with the quasi‐linear theory diffusion coefficients for low amplitude incoherent waves with (Bw,rms/B0)2≈3.7·10−10, over a time scale T of the order of 1,000 gyroperiods. However, the resonant interactions with higher amplitude waves cause significant nondiffusive dynamics as well as diffusive dynamics. When electron dynamics are extracted and analyzed over time scales shorter than T, we are able to isolate both the diffusive and nondiffusive (advective) dynamics. Interestingly, when considered over these appropriately shorter time scales (of the order of hundreds or tens of gyroperiods), the diffusive component of the dynamics agrees well with the predictions of quasi‐linear theory, even for wave amplitudes up to (Bw,rms/B0)2≈5.8·10−6. Quasi‐linear theory is based on fundamentally diffusive dynamics, but the evidence presented herein also indicates the existence of a distinct advective component. Therefore, the proper description of electron dynamics in response to wave‐particle interactions with higher amplitude whistler‐mode waves may require Fokker‐Planck equations that incorporate diffusive and advective terms.
Height Dependency of Solar Eclipse Effects: The Ionospheric Perspective
Authors: Verhulst Tobias G. W. , Stankov Stanimir M.
Published: June 2020

A reliable interpretation of solar eclipse effects on the geospace environment, and on the ionosphere in particular, necessitates a careful consideration of the so‐called eclipse geometry. A solar eclipse is a relatively rare astronomical phenomenon, which geometry is rather complex, specific for each event, and fast changing in time. The standard, most popular way to look at the eclipse geometry is via the two‐dimensional representation (map) of the solar obscuration on the Earth's surface, in which the path of eclipse totality is drawn together with isolines of the gradually‐decreasing eclipse magnitude farther away from this path. Such “surface maps” are widely used to readily explain some of the solar eclipse effects including, for example, the well‐known decrease in total ionization (due to the substantial decrease in solar irradiation), usually presented by the popular and easy to understand ionospheric characteristic of total electron content (TEC). However, many other effects, especially those taking place at higher altitudes, cannot be explained in this fashion. Instead, a more detailed description of the umbra (and penumbra), would be required. This paper addresses the issue of eclipse geometry effects on various ionospheric observations carried out during the total solar eclipse of 21 August 2017. In particular, GPS‐based TEC measurements were analyzed and eclipse effects on the ionosphere are interpreted with respect to the actual eclipse geometry at ionospheric heights. Whenever possible, a comparison was made with results from other eclipse events.
ΔNp63α-induced DUSP4/GSK3β/SNAI1 pathway in epithelial cells drives endometrial fibrosis
Authors: Zhao Guangfeng , Li Ruotian , Cao Yun , Song Minmin , Jiang Peipei , Wu Qianwen , Zhou Zhenhua , Zhu Hui , Wang Huiyan , Dai Chenyan , Liu Dan , Yao Simin , Lv Haining , Wang Limin , Dai Jianwu , Zhou Yan , Hu Yali
Published: June 2020

Epithelial homeostasis plays an essential role in maintaining endometrial function. But the epithelial role in endometrial fibrosis has been less studied. Previously, we showed that ectopic expression of ΔNp63α is associated with fibrosis process and epithelial dysfunction in endometria of patients with intrauterine adhesions (IUAs). Since ΔNp63α is profoundly involved in maintaining the epithelial homeostasis, we hereby focused on its roles in regulating the function and phenotype of endometrial epithelial cells (EECs) in context of endometrial fibrosis. We identified a typical type 2 epithelial-to-mesenchymal transition (EMT) in EECs from IUA patients and this process was induced by the forced expression of ΔNp63α in EECs. In transcriptomic analysis, we found that diverse signaling pathways regulated by ΔNp63α were involved in pro-EMT. We demonstrated that the DUSP4/GSK-3β/SNAI1 pathway was critical in transducing the pro-EMT signals initiated by ΔNp63α, while bFGF reversed ΔNp63α-induced EMT and endometrial fibrosis both in vitro and in vivo by blocking DUSP4/GSK3β/SNAI1 pathway. Taken together, our findings are important to understand the molecular mechanisms of endometrial fibrosis and to provide potential therapeutic targets.
Author Correction: Involvement of G-quadruplex regions in mammalian replication origin activity
Authors: Prorok Paulina , Artufel Marie , Aze Antoine , Coulombe Philippe , Peiffer Isabelle , Lacroix Laurent , Guédin Aurore , Mergny Jean-Louis , Damaschke Julia , Schepers Aloys , Cayrou Christelle , Teulade-Fichou Marie-Paule , Ballester Benoit , Méchali Marcel
Published: June 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Publisher Correction: Control of electron–electron interaction in graphene by proximity screening
Authors: Kim M. , Xu S. G. , Berdyugin A. I. , Principi A. , Slizovskiy S. , Xin N. , Kumaravadivel P. , Kuang W. , Hamer M. , Kumar R. Krishna , Gorbachev R. V. , Watanabe K. , Taniguchi T. , Grigorieva I. V. , Fal’ko V. I. , Polini M. , Geim A. K.
Published: June 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Permo–Triassic boundary carbon and mercury cycling linked to terrestrial ecosystem collapse
Authors: Dal Corso Jacopo , Mills Benjamin J. W. , Chu Daoliang , Newton Robert J. , Mather Tamsin A. , Shu Wenchao , Wu Yuyang , Tong Jinnan , Wignall Paul B.
Published: June 2020

Records suggest that the Permo–Triassic mass extinction (PTME) involved one of the most severe terrestrial ecosystem collapses of the Phanerozoic. However, it has proved difficult to constrain the extent of the primary productivity loss on land, hindering our understanding of the effects on global biogeochemistry. We build a new biogeochemical model that couples the global Hg and C cycles to evaluate the distinct terrestrial contribution to atmosphere–ocean biogeochemistry separated from coeval volcanic fluxes. We show that the large short-lived Hg spike, and nadirs in δ202Hg and δ13C values at the marine PTME are best explained by a sudden, massive pulse of terrestrial biomass oxidation, while volcanism remains an adequate explanation for the longer-term geochemical changes. Our modelling shows that a massive collapse of terrestrial ecosystems linked to volcanism-driven environmental change triggered significant biogeochemical changes, and cascaded organic matter, nutrients, Hg and other organically-bound species into the marine system.
Type 1 diabetes genetic risk score is discriminative of diabetes in non-Europeans: evidence from a study in India
Authors: Harrison James W. , Tallapragada Divya Sri Priyanka , Baptist Alma , Sharp Seth A. , Bhaskar Seema , Jog Kalpana S. , Patel Kashyap A. , Weedon Michael N. , Chandak Giriraj R. , Yajnik Chittaranjan S. , Oram Richard A.
Published: June 2020

Type 1 diabetes (T1D) is a significant problem in Indians and misclassification of T1D and type 2 diabetes (T2D) is a particular problem in young adults in this population due to the high prevalence of early onset T2D at lower BMI. We have previously shown a genetic risk score (GRS) can be used to discriminate T1D from T2D in Europeans. We aimed to test the ability of a T1D GRS to discriminate T1D from T2D and controls in Indians. We studied subjects from Pune, India of Indo-European ancestry; T1D (n = 262 clinically defined, 200 autoantibody positive), T2D (n = 345) and controls (n = 324). We used the 9 SNP T1D GRS generated in Europeans and assessed its ability to discriminate T1D from T2D and controls in Indians. We compared Indians with Europeans from the Wellcome Trust Case Control Consortium study; T1D (n = 1963), T2D (n = 1924) and controls (n = 2938). The T1D GRS was discriminative of T1D from T2D in Indians but slightly less than in Europeans (ROC AUC 0.84 v 0.87, p < 0.0001). HLA SNPs contributed the majority of the discriminative power in Indians. A T1D GRS using SNPs defined in Europeans is discriminative of T1D from T2D and controls in Indians. As with Europeans, the T1D GRS may be useful for classifying diabetes in Indians.
Improved drag coefficient and settling velocity for carbonate sands
Authors: Riazi Amin , Vila-Concejo Ana , Salles Tristan , Türker Umut
Published: June 2020

Sediment transport calculations are used globally in the numerical models that coastal managers, scientists and engineers use to assess and forecast coastal change. Most of the existing sediment transport equations were defined based on experimental results using siliciclastic sands. Yet these equations are applied to all types of sand, including carbonate sands that have different characteristics and therefore, settling behaviour. A rigorous management of the transport of carbonate sand is essential for the present and future management of sedimentary features in coral reefs such as sandy beaches or reef islands. Here we present a new approach to estimating the drag coefficient of carbonate sands that considers both friction and pressure. Based on our new method, the calculated drag coefficients explain the great variability in settling velocities of carbonate sand observed in nature (from 0.025 m/s to 0.364 m/s in our database). Using our formula, we demonstrate that even small differences in the settling velocity obtained with the new drag coefficient can lead to substantial changes in sediment transport and call for an update of numerical models.
Comparison of phosphor screen autoradiography and micro-pattern gas detector based autoradiography for the porosity of altered rocks
Authors: Delayre C. , Sammaljärvi J. , Billon S. , Muuri E. , Sardini P. , Siitari-Kauppi M.
Published: June 2020

This study aims to further develop the 14C-PMMA porosity calculation method with a novel autoradiography technique, the Micro-pattern gas detector autoradiography (MPGDA). In this study, the MPGDA is compared with phosphor screen autoradiography (SPA). A set of rock samples from Martinique Island exhibiting a large range of connected porosities was used to validate the MPGDA method. Calculated porosities were found to be in agreement with ones from the SPA and the triple-weight method (TW). The filmless nature of MPGDA as well as straightforward determination of C-14 radioactivity from the source rock makes the porosity calculation less uncertain. The real-time visualization of radioactivity from C-14 beta emissions by MPGDA is a noticeable improvement in comparison to SPA.
Spatio-temporal trends in richness and persistence of bacterial communities in decline-phase water vole populations
Authors: Villette Petra , Afonso Eve , Couval Geoffroy , Levret Aurélien , Galan Maxime , Goydadin Anne-Claude , Cosson Jean-François , Giraudoux Patrick
Published: June 2020

Understanding the driving forces that control vole population dynamics requires identifying bacterial parasites hosted by the voles and describing their dynamics at the community level. To this end, we used high-throughput DNA sequencing to identify bacterial parasites in cyclic populations of montane water voles that exhibited a population outbreak and decline in 2014–2018. An unexpectedly large number of 155 Operational Taxonomic Units (OTUs) representing at least 13 genera in 11 families was detected. Individual bacterial richness was higher during declines, and vole body condition was lower. Richness as estimated by Chao2 at the local population scale did not exhibit clear seasonal or cycle phase-related patterns, but at the vole meta-population scale, exhibited seasonal and phase-related patterns. Moreover, bacterial OTUs that were detected in the low density phase were geographically widespread and detected earlier in the outbreak; some were associated with each other. Our results demonstrate the complexity of bacterial community patterns with regard to host density variations, and indicate that investigations about how parasites interact with host populations must be conducted at several temporal and spatial scales: multiple times per year over multiple years, and at both local and long-distance dispersal scales for the host(s) under consideration.
Spatially-resolved metabolic profiling of living Drosophila in neurodegenerative conditions using 1H magic angle spinning NMR
Authors: Yon Maxime , Decoville Martine , Sarou-Kanian Vincent , Fayon Franck , Birman Serge
Published: June 2020

Drosophila flies are versatile animal models for the study of gene mutations in neuronal pathologies. Their small size allows performing in vivo Magic Angle Spinning (MAS) experiments to obtain high-resolution 1H nuclear magnetic resonance (NMR) spectra. Here, we use spatially-resolved 1H high-resolution MAS NMR to investigate in vivo metabolite contents in different segments of the fly body. A comparative study of metabolic changes was performed for three neurodegenerative disorders: two cell-specific neuronal and glial models of Huntington disease (HD) and a model of glutamate excitotoxicity. It is shown that these pathologies are characterized by specific and sometimes anatomically localized variations in metabolite concentrations. In two cases, the modifications of 1H MAS NMR spectra localized in fly heads were significant enough to allow the creation of a predictive model.
Vagus nerve stimulation even after injury ameliorates cisplatin-induced nephropathy via reducing macrophage infiltration
Authors: Uni Rie , Inoue Tsuyoshi , Nakamura Yasuna , Fukaya Daichi , Hasegawa Sho , Wu Chia-Hsien , Fujii Rie , Surattichaiyakul Bongkod , Peerapanyasut Wachirasek , Ozeki Atsuko , Akimitsu Nobuyoshi , Wada Youichiro , Nangaku Masaomi , Inagi Reiko
Published: June 2020

The efficacy of prior activation of an anti-inflammatory pathway called the cholinergic anti-inflammatory pathway (CAP) through vagus nerve stimulation (VNS) has been reported in renal ischemia-reperfusion injury models. However, there have been no reports that have demonstrated the effectiveness of VNS after injury. We investigated the renoprotective effect of VNS in a cisplatin-induced nephropathy model. C57BL/6 mice were injected with cisplatin, and VNS was conducted 24 hours later. Kidney function, histology, and a kidney injury marker (Kim-1) were evaluated 72 hours after cisplatin administration. To further explore the role of the spleen and splenic macrophages, key players in the CAP, splenectomy, and adoptive transfer of macrophages treated with the selective α7 nicotinic acetylcholine receptor agonist GTS-21 were conducted. VNS treatment significantly suppressed cisplatin-induced kidney injury. This effect was abolished by splenectomy, while adoptive transfer of GTS-21-treated macrophages improved renal outcomes. VNS also reduced the expression of cytokines and chemokines, including CCL2, which is a potent chemokine attracting monocytes/macrophages, accompanied by a decline in the number of infiltrating macrophages. Taken together, stimulation of the CAP protected the kidney even after injury in a cisplatin-induced nephropathy model. Considering the feasibility and anti-inflammatory effects of VNS, the findings suggest that VNS may be a promising therapeutic tool for acute kidney injury.
Dose-dependent pro- or anti-fibrotic responses of endometriotic stromal cells to interleukin-1β and tumor necrosis factor α
Authors: Matsuzaki Sachiko , Pouly Jean-Luc , Canis Michel
Published: June 2020

Endometriosis are characterized by dense fibrous tissue. Numerous studies have investigated roles of inflammation on the pathophysiology of endometriosis. However, the interplay of inflammation and fibrosis remains to be clarified. Here we show that low levels of interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNFα) promoted a fibrotic phenotype, whereas high levels of IL-1β and TNFα inactivated the fibrotic phenotype of endometriotic stromal cells (Ectopic-ES). IL-1β 10 pg/mL and TNFα 100 and 1,000 pg/mL had minimal effects, whereas the highest dose of IL-1β (100 pg/mL) significantly decreased collagen gel contraction in Ectopic-ES. Furthermore, in Ectopic-ES, low levels of IL-1β (1 pg/mL) and/or TNFα 10 pg/mL significantly increased Col I mRNA expression, whereas higher doses of IL-1β (10 and/or 100 pg/mL) and/or TNFα (100 and/or 1,000 pg/mL) significantly decreased Col I and/or αSMA mRNA expression and the percentage of cells with Col I + and/or αSMA + stress fibers. In contrast, in either menstrual endometrial stromal cells of patients with endometriosis or those of healthy women, varying doses of IL-1β and/or TNFα had no significant effects on either Col I or αSMA mRNA/protein expression. The present findings bring into question whether we should still continue to attempt anti-inflammatory treatment strategies for endometriosis.
A Retrospective Analysis Of Different Contingent Screening Models For Fetal Down Syndrome In Southwestern China
Authors: Luo Wei , He Bin , Han Daiwen , Yuan Lixing , Chen Xinlian , Pang Ling , Tang Jun , Zou Fene , Zhao Kai , Du Yepei , Liu Hongqian
Published: June 2020

To discuss combinations of traditional screening and noninvasive prenatal screening (NIPS) and to compare which traditional screening is the most suitable first-line screening approach to NIPS, pregnant women were recruited in this retrospective observational study. Pregnant women underwent one of four traditional screening tests. The 9 contingent models were combined by high risk cut-offs of 1:50, 1:100, 1:270 and intermediate risk cut-offs of 1:1000, 1:1500, 1:2000. We analyzed cost and performance of various screening models with contingent screening of different risk cut-offs. Compared with other screening tests, combined first-trimester screening (CFTS) had the lowest proportion of high risk (≥1:270) with the highest detection rate (DR) (78.79%) and the lowest proportion of intermediate risk (1:271~1:1000). When intermediate risk was 1:51 ~1:1500, CFTS as first-line screening had the lowest cost with DR of 93.94%. Other screening tests as the first-line screening with intermediate risk of 1:51~1:1000 had the lowest cost, there DR were 90.91%, 84.62%, 91.67%, respectively. Our study demonstrated if only one traditional screening was allowed to screen pregnant women, CFTS was recommended as the first choice. According to local health and economic conditions, adopting appropriate traditional screening with suitable cut-offs as first-line screening will contributed to a cost-effective screening model.
Acellular bioscaffolds redirect cardiac fibroblasts and promote functional tissue repair in rodents and humans with myocardial injury
Authors: Svystonyuk Daniyil A. , Mewhort Holly E. M. , Hassanabad Ali Fatehi , Heydari Bobak , Mikami Yoko , Turnbull Jeannine D. , Teng Guoqi , Belke Darrell D. , Wagner Karl T. , Tarraf Samar A. , DiMartino Elena S. , White James A. , Flewitt Jacqueline A. , Cheung Matthew , Guzzardi David G. , Kang Sean , Fedak Paul W. M.
Published: June 2020

Coronary heart disease is a leading cause of death. Tissue remodeling and fibrosis results in cardiac pump dysfunction and ischemic heart failure. Cardiac fibroblasts may rebuild damaged tissues when prompted by suitable environmental cues. Here, we use acellular biologic extracellular matrix scaffolds (bioscaffolds) to stimulate pathways of muscle repair and restore tissue function. We show that acellular bioscaffolds with bioinductive properties can redirect cardiac fibroblasts to rebuild microvascular networks and avoid tissue fibrosis. Specifically, when human cardiac fibroblasts are combined with bioactive scaffolds, gene expression is upregulated and paracrine mediators are released that promote vasculogenesis and prevent scarring. We assess these properties in rodents with myocardial infarction and observe bioscaffolds to redirect fibroblasts, reduce tissue fibrosis and prevent maladaptive structural remodeling. Our preclinical data confirms that acellular bioscaffold therapy provides an appropriate microenvironment to stimulate pathways of functional repair. We translate our observations to patients with coronary heart disease by conducting a first-in-human observational cohort study. We show that bioscaffold therapy is associated with improved perfusion of infarcted myocardium, reduced myocardial scar burden, and reverse structural remodeling. We establish that clinical use of acellular bioscaffolds is feasible and offers a new frontier to enhance surgical revascularization of ischemic heart muscle.
A cognitive analysis of deceptive pollination: associative mechanisms underlying pollinators’ choices in non-rewarding colour polymorphic scenarios
Authors: Aguiar João Marcelo Robazzi Bignelli Valen , Giurfa Martin , Sazima Marlies
Published: June 2020

Intraspecific floral colour polymorphism is a common trait of food deceptive orchids, which lure pollinators with variable, attractive signals, without providing food resources. The variable signals are thought to hinder avoidance learning of deceptive flowers by pollinators. Here, we analysed the cognitive mechanisms underlying the choice of free-flying stingless beesScaptotrigonaaff.depilistrained to visit a patch of artificial flowers that displayed the colours ofIonopsis utricularioides, a food deceptive orchid. Bees were trained in the presence of a non-rewarding colour and later tested with that colour vs. alternative colours. We simulated a discrete-polymorphism scenario with two distinct non-rewarding test colours, and a continuous-polymorphism scenario with three non-rewarding test colours aligned along a chromatic continuum. Bees learned to avoid the non-rewarding colour experienced during training. They thus preferred the novel non-rewarding colour in the discrete-polymorphic situation, and generalized their avoidance to the adjacent colour of the continuum in the continuous-polymorphism situation, favouring thereby the most distant colour. Bees also visited less flowers and abandoned faster a non-rewarding monomorphic patch than a non-rewarding polymorphic patch. Our cognitive analyses thus reveal that variable deceptive orchids disrupt avoidance learning by pollinators and exploit their generalization abilities, which make them favour distinct morphs.
Seasonal variation in net ecosystem CO2 exchange of a Brazilian seasonally dry tropical forest
Authors: Mendes Keila R. , Campos Suany , da Silva Lindenberg L. , Mutti Pedro R. , Ferreira Rosaria R. , Medeiros Salomão S. , Perez-Marin Aldrin M. , Marques Thiago V. , Ramos Tarsila M. , de Lima Vieira Mariana M. , Oliveira Cristiano P. , Gonçalves Weber A. , Costa Gabriel B. , Antonino Antonio C. D. , Menezes Rômulo S. C. , Bezerra Bergson G. , Santos e Silva Cláudio M.
Published: June 2020

Forest ecosystems sequester large amounts of atmospheric CO2, and the contribution from seasonally dry tropical forests is not negligible. Thus, the objective of this study was to quantify and evaluate the seasonal and annual patterns of CO2 exchanges in the Caatinga biome, as well as to evaluate the ecosystem condition as carbon sink or source during years. In addition, we analyzed the climatic factors that control the seasonal variability of gross primary production (GPP), ecosystem respiration (Reco) and net ecosystem CO2 exchange (NEE). Results showed that the dynamics of the components of the CO2 fluxes varied depending on the magnitude and distribution of rainfall and, as a consequence, on the variability of the vegetation state. Annual cumulative NEE was significantly higher (p < 0.01) in 2014 (−169.0 g C m−2) when compared to 2015 (−145.0 g C m−2) and annual NEP/GPP ratio was 0.41 in 2014 and 0.43 in 2015. Global radiation, air and soil temperature were the main factors associated with the diurnal variability of carbon fluxes. Even during the dry season, the NEE was at equilibrium and the Caatinga acted as an atmospheric carbon sink during the years 2014 and 2015.
Electrochemical Immunoassay for Determination of Glycated Albumin using Nanozymes
Authors: Choi Hyun , Son Seong Eun , Hur Won , Tran Van-Khue , Lee Han Been , Park Yosep , Han Do Kyoung , Seong Gi Hun
Published: June 2020

We developed a new nanozyme-based electrochemical immunoassay method for the monitoring of glycated albumin (GA) known to reflect short-term glycaemic levels. For this study, we synthesized urchin-like Pt nanozymes (uPtNZs) and applied them to colorimetric and electrochemical assays for sensitive determination of GA in total human serum albumin (tHSA) using 3,3′,5,5′-tetramethylbenzidine (TMB) and thionine as substrates, respectively. The uPtNZs showed peroxidase-mimic activity in the presence of hydrogen peroxide. Boronic acid (BA)-agarose bead was used to capture GA through specific cis-diol interactions. uPtNZs were modified with GA antibody (GA-Ab) to form sandwich complexes with GA/BA-agarose bead. The amount of Ab-uPtNZ/GA/BA-agarose bead complex increased with increasing percentage of GA in 50 mg/mL tHSA. The colorimetric assay exhibited linearity from 0.02 to 10% (10 µg/mL – 5 mg/mL) GA with an LOD of 0.02% (9.2 µg/mL). For electrochemical assay, GA was detected from 0.01 to 20% (5 µg/mL – 10 mg/mL) with an LOD of 0.008% (3.8 µg/mL). The recovery values of measured GA in human plasma samples were from 106 to 107%. These results indicate that electrochemical assay using uPtNZs is a promising method for determining GA.