bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality of Life Assessment in Students from Polish Universities during the COVID-19 Pandemic According to WHO Quality of Life Questionnaire
Authors: Trzcionka Agata , Włodarczyk-Sielicka Marta , Surmiak Piotr , Szymańska Anna , Pohl Artur , Tanasiewicz Marta
Published: July 2022

The outbreak of COVID-19 in December 2019 in China influenced the lives of people all over the world. Many had to face the completely new situation of lockdown. These changes influenced many aspects of life. Students’ quality of life changed as well. The aim of the study was to assess the differences in the quality of life of students with regard to the field of study and the knowledge regarding medicine. The study population consisted of 500 students from three Polish universities (Medical University of Silesia, Maritime University of Szczecin and Adam Mickiewicz University, Poznań). Study participants were invited to fill in an online cross-sectional quality of life questionnaire (WHOQOL-BREF) created by the World Health Organization (WHO). The analysis was done using the IBM SPSS Statistics 25.0 programme. The obtained results showed differences in respondents’ reactions in two domains. The lowest resistance to the critical situation was observed in women who studied at the technical university. Higher values of resistance were observed in women studying medical sciences.
The Potential Key Role of the NRF2/NQO1 Pathway in the Health Effects of Arsenic Pollution on SCC
Authors: Yang Qianlei , Yan Rui , Mo Yuemei , Xia Haixuan , Deng Hanyi , Wang Xiaojuan , Li Chunchun , Kato Koichi , Zhang Hengdong , Jin Tingxu , Zhang Jie , An Yan
Published: July 2022

Arsenic is widely present in nature and is a common environmental poison that seriously damages human health. Chronic exposure to arsenic is a major environmental poisoning factor that promotes cell proliferation and leads to malignant transformation. However, its molecular mechanism remains unclear. In this study, we found that arsenite can promote the transformation of immortalized human keratinocyte cells (HaCaT) from the G0/G1 phase to S phase and demonstrated malignant phenotypes. This phenomenon is accompanied by obviously elevated levels of NRF2, NQO1, Cyclin E, and Cyclin-dependent kinase 2 (CDK2). Silencing the NRF2 expression with small interfering RNA (siRNA) in arsenite-transformed (T-HaCaT) cells was shown to reverse the malignant phenotype. Furthermore, the siRNA silencing of NQO1 significantly decreased the levels of the cyclin E-CDK2 complex, inhibiting the G0/G1 to S phase cell cycle progression and transformation to the T-HaCaT phenotypes. Thus, we hypothesized that the NRF2/NQO1 pathway played a key role in the arsenite-induced malignancy of HaCaT cells. By increasing the expression of Cyclin E-CDK2, the NRF2/NQO1 pathway can affect cell cycle progression and cell proliferation. A new common health effect mechanism of arsenic carcinogenesis has been identified; thus, it would contribute to the development of novel treatments to prevent and treat skin cancer caused by arsenic.
Use of Blockchain Technology in the Domain of Physical Exercise, Physical Activity, Sport, and Active Ageing: A Systematic Review
Authors: Lopez-Barreiro Juan , Alvarez-Sabucedo Luis , Garcia-Soidan Jose Luis , Santos-Gago Juan M.
Published: July 2022

Blockchain technology provides a distributed support for information storage and traceability. Recently, it has been booming in a wide variety of domains: finance, food, energy, and health. In the field of physical activity, physical exercise, sport, and active ageing, this technology could also originate some interesting services introducing support for reliable repository of results, for gamification, or for secure data interchange. This systematic review explores the use of blockchain in this context. The objective is to determine to which extent this technology has fulfilled the potential of blockchain to bring these new added-value services. The authors explored 5 repositories in search of papers describing solutions applied to the above-mentioned frame. 17 papers were selected for full-text analysis, and they displayed diverse applications of blockchain, such as Fitness and healthcare, Sport, and Active ageing. A detailed analysis shows that the solutions found do not leverage all the possibilities of blockchain technology. Most of the solutions analyzed use blockchain for managing, sharing, and controlling access to data and do not exploit the possibilities of Smart Contracts or oracles. Additionally, the advantages of the blockchain model have not been fully exploited to engage users using approaches such as gamification.
A Participatory-Based Research Approach for Assessing Exposure to Lead-Contaminated Drinking Water in the Houston Neighborhood of the Greater Fifth Ward
Authors: Fawkes Leanne S. , McDonald Thomas J. , Roh Taehyun , Chiu Weihsueh A. , Taylor Robert J. , Sansom Garett T.
Published: July 2022

To address community-driven concerns about lead-contaminated drinking water in residential homes in the Greater Fifth Ward neighborhood in Northeast Houston, Texas utilizing participatory-based research. The study collected survey data and performed lead analysis on drinking water from residents’ homes. The Greater Fifth Ward is characterized as a majority-minority environmental justice community and is located within two confirmed cancer clusters. The residents of 172 homes completed a survey and had detectable lead levels in their water samples. Survey results indicated that more than half of the residents (58.2%) were concerned with the water quality and 42.9% rated the drinking water as poor. Water lead levels detected ranged from 0.01 to 22 µg/L. 10.9% of homes exceeding 1 µg/L, and one located exceeded the USEPA’s action limit of 15 µg/L. Homes built prior to 1978 without major renovation had significantly higher levels of lead in their drinking water compared to homes built after 1978 (p-value < 0.05). These findings demonstrate the need for lead testing of residential water in low socioeconomic-status communities, as well as demonstrating the benefits of community engagement and participatory research to address environmental health concerns.
The Occurrence of Microplastics and the Formation of Biofilms by Pathogenic and Opportunistic Bacteria as Threats in Aquaculture
Authors: Cholewińska Paulina , Moniuszko Hanna , Wojnarowski Konrad , Pokorny Przemysław , Szeligowska Natalia , Dobicki Wojciech , Polechoński Ryszard , Górniak Wanda
Published: July 2022

Aquaculture is the most rapidly growing branch of animal production. The efficiency and quality of the produced food depends on sustainable management, water quality, feed prices and the incidence of diseases. Micro- (MP < 5 mm) and nanoplastic (NP < 1000 nm) particles are among the current factors causing serious water pollution. This substance comes solely from products manufactured by humans. MP particles migrate from the terrestrial to the aquatic environment and adversely affect, especially, the health of animals and humans by being a favorable habitat and vector for microbial pathogens and opportunists. More than 30 taxa of pathogens of humans, aquacutural animals and plants, along with opportunistic bacteria, have been detected in plastic-covering biofilm to date. The mobility and durability of the substance, combined with the relatively closed conditions in aquacultural habitats and pathogens’ affinity to the material, make plastic particles a microbiological medium threatening the industry of aquaculture. For this reason, in addition to the fact of plastic accumulation in living organisms, urgent measures should be taken to reduce its influx into the environment. The phenomenon and its implications are related to the concept of one health, wherein the environment, animals and humans affect each other’s fitness.
WALES 2021 Active Healthy Kids (AHK) Report Card: The Fourth Pandemic of Childhood Inactivity
Authors: Richards Amie B. , Mackintosh Kelly A. , Swindell Nils , Ward Malcolm , Marchant Emily , James Michaela , Edwards Lowri C. , Tyler Richard , Blain Dylan , Wainwright Nalda , Nicholls Sarah , Mannello Marianne , Morgan Kelly , Evans Tim , Stratton Gareth
Published: July 2022

This is the fourth Active Healthy Kids (AHK) Wales Report Card. The 2021 card produced grades on children and young people’s physical activity (PA) using pre-COVID-19 data that were not used in previous versions. Eleven quality indicators of PA were graded through expert consensus and synthesis of the best available evidence. Grades were assigned as follows: Overall PA—F; Organised Sport and PA—C; Active Play—C+; Active Transportation—C−; Sedentary Behaviours—F; Physical Fitness—C−; Family and Peer Influences—D+; School—B−; Community and the Built Environment—C; National Government and Policy—C; and Physical Literacy—C−. All but three grades remained the same or decreased from the 2018 AHK-Wales Report Card (Active Play increased from C− to C+; Active Transportation, D+ to C−; Family and Peers, D to D+). This is concerning for children’s health and well-being in Wales, particularly given recent evidence that PA has further decreased during the COVID-19 pandemic. The results from the Report Card should be used to inform the decision making of policy makers, practitioners and educators to improve children and young people’s PA levels and opportunities and decrease PA inequalities.
High Incidence of Partial Biotinidase Deficiency in the First 3 Years of a Regional Newborn Screening Program in Italy
Authors: Semeraro Daniela , Verrocchio Sara , Di Dalmazi Giulia , Rossi Claudia , Pieragostino Damiana , Cicalini Ilaria , Ferrante Rossella , Di Michele Silvia , Stuppia Liborio , Rizzo Cristiano , Lepri Francesca Romana , Novelli Antonio , Dionisi-Vici Carlo , De Laurenzi Vincenzo , Bucci Ines
Published: July 2022

Biotinidase deficiency (BD) is an autosomal recessive inherited disorder in which the enzyme biotinidase is totally or partially defective and the vitamin biotin is not recycled. BD meets the major criteria for a population screening program. Newborn bloodspot screening (NBS) allows early diagnosis of BD, thus preventing the high morbidity and mortality associated with untreated disease. Both profound and partial BD variant can be detected by NBS test, and serum enzyme activity and/or mutational analysis are required for definitive diagnosis. In Italy, BD is included in the screening panel for inborn errors of metabolism (IEMs) that has been declared mandatory in 2016. We analyzed the data of the first 3 years of the NBS for BD in our region (Abruzzo, Italy), with the aim to describe the outcomes of this recently introduced screening program. In over 26,393 newborns screened, we found 2 carriers and 16 cases with genotype associated with partial BD. Since the serum biotinidase assay has been recently introduced in our algorithm, only three of our newborns met the criteria of genetic and biochemical confirmation, with an incidence of 1:8797, which is in the high range of what has been reported in the literature. All affected infants carried the 1330G>C (D444H) variant in compound heterozygosis, with variants known to be associated with profound BD. A variant previously not described and likely pathogenic was found in one newborn. None of the infants had signs or symptoms. The study of the distribution of the enzyme activity in our population allowed us to validate the adopted cutoff with which the program has a positive predictive value of 18% and to analyze some preanalytical factors influencing biotinidase activity: A correlation of the enzyme activity with gestational age and time at specimen collection was found. Lower mean values of enzyme activity were found in infants born in the summer.
Effects of Municipal Solid Waste on Planting Properties and Scouring Resistance of Vegetation Concrete (Wuhan, China)
Authors: Kong Liulin , Wang Xiaomei , Guo Wencheng , Zhang Yongcheng
Published: July 2022

Vegetation concrete (VC) laid as a reinforcement base and covered by a soil layer with vegetation has been increasingly used to beautify the landscape, reduce environmental pollution and control stormwater runoff. In this study, the effects of municipal solid waste (MSW) on vegetation characteristics of modified VC were tested under different mix compositions. We first explored the effects of the mixed concrete environment on Festuca elata and perennial Ryegrass for 60 days. Then, the influence of various MSW contents added to different percentages of cement on scouring resistance of VC was examined. The experimental results revealed that the germination rates and plant heights of both species decreased with the increase in concrete content. Considering the scouring resistances, the optimal mix proportion of MSW-modified VC was recommended as No. 25, with 5% KW fertilizer, 8% cement and 0.5% wheat straw in this study. Furthermore, adding a small amount of fallen leaves or silica fume to VC can promote the growth of both species to some extent, although these additions had an inverse effect on the scouring resistances. The results contribute to beneficial knowledge for future research on the feasibility of the use these species with VC technology for slope ecological restoration.
Land Fragmentation, Technology Adoption and Chemical Fertilizer Application: Evidence from China
Authors: Chi Liang , Han Shuqing , Huan Meili , Li Yajuan , Liu Jifang
Published: July 2022

Although it has been widely recognized that land fragmentation has increased chemical fertilizer application, little is known about the role of technology adoption in mitigating these adverse effects. To empirically examine the relationship between land fragmentation, technology adoption and chemical fertilizer application, we developed a mediation model. We applied our analysis to a survey data set encompassing 1388 farm-level samples collected in 14 Chinese provinces in 2019. Our study demonstrated that land fragmentation can not only directly increase chemical fertilizer application but also indirectly increase it by hindering the adoption of agricultural mechanization technologies (AMT’s) and soil testing fertilization technologies (STFT’s). Both are recognized as potent drivers of fertilizer use reductions. Moreover, the adoption of information and communications technologies (ICT’s) can help mitigate the negative effects of land fragmentation on technology adoption, thus reducing chemical fertilizer application intensity (CFAI). However, the direct effects of land fragmentation on CAFI was unaffected by ICT’s. Our findings suggest that ICT’s have revolutionized farmer recognition, promotion and adoption of agricultural technologies by increasing awareness and diffusion of agricultural technology information.
Research Capacity Training on Environmental Health and Noncommunicable Diseases in the Country of Georgia: Challenges and Lessons Learned during the COVID-19 Pandemic
Authors: Berg Carla J. , Sturua Lela , Marsit Carmen J. , Baramidze Levan , Kiladze Nino , Caudle William Michael
Published: July 2022

COVID-19 presented challenges for global health research training programs. The Clean Air Research and Education (CARE) program, which aims to enhance research capacity related to noncommunicable diseases and environmental health in the country of Georgia, was launched in 2020—as the COVID-19 pandemic began. At its foundation is mentorship and mentored research, alongside formal didactic training, informal training/meetings, and other supports. Current analyses examined CARE’s initial 1.5 years (e.g., program benefits, mentorship relationships) using data from an evaluation survey among trainees and faculty in January 2022. Trainees (100% response rate: n = 12/12; 4 MPH, 8 PhD) and faculty (86.7% response rate: n = 13/15; 7 Georgia-based, 6 United States-based) rated factors related to mentor-mentee relationships highly, particularly mutual consideration of each other’s thoughts, opinions, and perspectives; one major challenge was completing goals planned. Trainees and faculty identified several growth experiences and program benefits (e.g., skills development, expanding professional network) but also identified challenges (e.g., meeting program demands, communication gaps, unclear expectations)—exacerbated by the pandemic. Findings underscore the importance of strong mentorship relationships and that the pandemic negatively impacted communication and clarity of expectations. Given the likely ongoing impact of the pandemic on such programs, program leaders must identify ways to address these challenges.
The Effects of Exercise on Acute Immune Responses in Relative Leisure-Deprived People Living with HIV/AIDS: A Pilot Study
Authors: Qin Xin-Min , Park Ji-Young , Kim Bo-Ram , Joo Chang-Hwa
Published: July 2022

Exercise training involving exercises of optimal intensity and duration improves psychological and medical variables in relative leisure-deprived people living with HIV/AIDS. This study aimed to analyze associated psychological variables and the effect of exercise intensity and duration on immune responses in relative leisure-deprived people infected with HIV. The participants completed different moderate-intensity exercises (30 min (60–80% HRmax) and 45 min (60–80% HRmax)) and high-intensity exercise for 10 min (>80% HRmax). Levels higher than “normal” were rated for relative leisure deprivation, indicating relative deprivation of leisure among participants. The overall level of quality of life was “normal”, indicating that quality of life was not considered high. The stress level was psychologically considered low. Time had a significant effect on cortisol levels (p < 0.05). Compared to pre-exercise, cortisol level was significantly decreased immediately after moderate exercise for 45 min and 3 h post-exercise after high-intensity exercise for 10 min (p < 0.05). However, time and the interaction of condition and time had no significant effect on IL-6 and sIgA levels (p > 0.05). Despite the small sample size of this pilot study, the results demonstrate that moderate-intensity exercise can be recommended to improve the health and quality of life of people infected with HIV.
Comparison of the Amount of Used and the Ease of Oral Care between Liquid and Gel-Type Oral Moisturizers Used with an Oral Care Simulators
Authors: Suzuki Hiroyuki , Furuya Junichi , Matsubara Chiaki , Aoyagi Michiyo , Shirobe Maki , Sato Yuji , Tohara Haruka , Minakuchi Shunsuke
Published: July 2022

Oral care involving the removal of dry sputum is effective for older patients who require nursing care or hospitalization. However, safe and efficient oral care methods for such patients remain unclear. We aimed to simulate the oral cavity of older adults with dry mouth and elucidate the differences between two moisturization agents, water and gel-like oral moisturizer, and investigate the effect of occupation and experience on the amount of use and the ease of oral care. Using an oral care simulator (MANABOT®, Nissin Dental Products Inc., Kyoto, Japan), 42 students and 48 dental professionals (13 dentists and 35 dental hygienists) performed oral care using moisturization agents to facilitate dry sputum removal. The time required for oral care, amount of water or gel used, amount of pharyngeal inflow, and ease of oral care when using water or gel were compared. The simulations revealed that the amount of use and pharyngeal inflow for gel (2.9 ± 1.6 and 0.3 ± 0.3, respectively) were significantly lower than those for water (6.8 ± 4.1 and 1.2 ± 1.5, respectively) in all participants. Using a gel-like moisturizer might reduce the aspiration risk in older patients requiring nursing care or hospitalization, regardless of occupation and experience.
Is Pollen Production of Birch Controlled by Genetics and Local Conditions?
Authors: Ranpal Surendra , Sieverts Miriam , Wörl Verena , Kahlenberg Georgia , Gilles Stefanie , Landgraf Maria , Köpke Kira , Kolek Franziska , Luschkova Daria , Heckmann Tobias , Traidl-Hoffmann Claudia , Büttner Carmen , Damialis Athanasios , Jochner-Oette Susanne
Published: July 2022

Intraspecific genetic variation might limit the relevance of environmental factors on plant traits. For example, the interaction between genetics and (a-)biotic factors regulating pollen production are still poorly understood. In this study, we investigated pollen production of 28 birch (Betula pendula Roth) individuals in the years 2019–2021. We sampled catkins of eleven groups of genetically identical trees, which were partially topped, but of the same age and located in a seed plantation in southern Germany characterized by similar microclimatic conditions. Furthermore, we monitored environmental factors such as air temperature, characterized air quality (NO2, NOx and O3), and assessed potential solar radiation. We especially checked for differences between years as well as between and within clones and assessed the synchronicity of years with high/low pollen production. We present a robust mean for the pollen production of Betula pendula (1.66 million pollen grains per catkin). Our findings show temporal (H(2) = 46.29, p < 0.001) and clonal variations (H(4) = 21.44, p < 0.001) in pollen production. We conclude that synchronized high or low pollen production is not utterly site-specific and, in addition, not strictly dependent on genotypes. We suggest that appropriate clone selection based on application (seed plantation, urban planting) might be advantageous and encourage a long-term monitoring.
The Relationship between Attachment Styles and Compulsive Online Shopping: The Mediating Roles of Family Functioning Patterns
Authors: Topino Eleonora , Cacioppo Marco , Gori Alessio
Published: July 2022

The rapid expansion of e-commerce has made the buying experience faster, potentially anonymous, and without limits of space and time. While this may produce benefits, for some individuals, online shopping can become an addiction. Therefore, the present study aimed to explore the psychological factors that may be associated with Compulsive Online Shopping, with a specific focus on the role of Attachment Styles and Family Functioning patterns as risk or protective factors. The study involved a sample of 306 participants (Mage = 31.86 years, SD = 11.925) who filled out an online survey consisting of the Compulsive Online Shopping Scale, Relationship Questionnaire, Family Adaptability and Cohesion Evaluation Scales-IV, as well as a demographic questionnaire. The results showed two significant parallel mediation models. In the first one, Secure Attachment was negatively and significantly related to Compulsive Online Shopping, with the mediation of Cohesion and Enmeshed Family Functioning. In the second one, Fearful Attachment was positively and significantly related to Compulsive Online Shopping, with the mediation of Cohesion and Enmeshed Family Functioning. Important implications for preventive activity and tailored interventions may emerge from these data.
The Recent Progress China Has Made in Green Mine Construction, Part II: Typical Examples of Green Mines
Authors: Yu Haoxuan , Li Shuai , Yu Lifeng , Wang Xinmin
Published: July 2022

This paper (Part II), right after the Part I, also as an information article, introduces the recent progress of “green mine construction” in China. China is a big country in resource exploitation, but there are serious problems such as hidden danger, environmental pollution and resource waste in the exploitation of mineral resources in China. Therefore, the promotion of “green” mining technology, the implementation of “green mine construction” and the promotion of small- and medium-sized mines to green non-waste mining mode transformation and upgrading are crucial measures on the road of China’s mining development, with very important practical significance. Therefore, this information paper of our Chinese mining research mainly reviews the key progress in the construction and development of green mines and introduces four typical green mine examples in China: (1) the mine with the best green environment in China: Jinhui Mining Co., Ltd., Jiuquan, Gansu Province; (2) the most advanced mine in China: Jinchuan Group Company, Jinchang, Gansu Province; (3) the oldest green mine in China: the Suichang gold mine, Lishui, Zhejiang Province; and (4) the most mechanized mine in China: the Pingshuo Coal Co., Ltd., Shuozhou, Shanxi Province. In the abstract, we claim that Part II serves as a guide to begin a conversation and to encourage experts and scholars to engage in the research of this field.
Palliative Effect of Resveratrol against Nanosized Iron Oxide-Induced Oxidative Stress and Steroidogenesis-Related Genes Dysregulation in Testicular Tissue of Adult Male Rats
Authors: Ahmed Mona M. , Hussein Mohamed M. A. , Saber Taisir , Abd-Elhakim Yasmina M.
Published: July 2022

The nano-sized iron oxide (Fe2O3-NPs) is one of the most used engineered nanomaterials worldwide. This study investigated the efficacy of natural polyphenol resveratrol (RSV) (20 mg/kg b.wt, orally once daily) to alleviate the impaired sperm quality and testicular injury resulting from Fe2O3-NPs exposure (3.5 or 7 mg/kg b.wt, intraperitoneally once a week) for eight weeks. Spermiograms, sexual hormonal levels, oxidative stress indicators, and lipid peroxidation biomarker were assessed. Moreover, the steroidogenesis-related genes mRNA expressions were evaluated. The results showed that RSV substantially rescued Fe2O3-NPs-mediated sperm defects. Additionally, the Fe2O3-NPs-induced depressing effects on sperm motility and viability were markedly counteracted by RSV. Moreover, RSV significantly restored Fe2O3-NPs-induced depletion of testosterone, follicle-stimulated hormone, luteinizing hormone, and testicular antioxidant enzymes but reduced malondialdehyde content. Furthermore, the Fe2O3-NPs-induced downregulation of steroidogenesis-related genes (3 β-HSD, 17 β-HSD, and Nr5A1) was significantly counteracted in the testicular tissue of RSV-treated rats. These findings concluded that RSV could limit the Fe2O3-NPs-induced reduced sperm quality and testicular injury most likely via their antioxidant activity and steroidogenesis-related gene expression modulation.
Behavioral Economics and Tobacco Control: Current Practices and Future Opportunities
Authors: Littman Dalia , Sherman Scott E. , Troxel Andrea B. , Stevens Elizabeth R.
Published: July 2022

Despite considerable progress, smoking remains the leading preventable cause of death in the United States. To address the considerable health and economic burden of tobacco use, the development of improved tobacco control and treatment interventions is critical. By combining elements of economics and psychology, behavioral economics provides a framework for novel solutions to treat smokers who have failed to quit with traditional smoking cessation interventions. The full range of behavioral economic principles, however, have not been widely utilized in the realm of tobacco control and treatment. Given the need for improved tobacco control and treatment, the limited use of other behavioral economic principles represents a substantial missed opportunity. For this reason, we sought to describe the principles of behavioral economics as they relate to tobacco control, highlight potential gaps in the behavioral economics tobacco research literature, and provide examples of potential interventions that use each principle.
Differences in Immunological Evasion of the Delta (B.1.617.2) and Omicron (B.1.1.529) SARS-CoV-2 Variants: A Retrospective Study on the Veneto Region’s Population
Authors: Cocchio Silvia , Zabeo Federico , Facchin Giacomo , Piva Nicolò , Venturato Giovanni , Marcon Thomas , Saia Mario , Tonon Michele , Mongillo Michele , Da Re Filippo , Russo Francesca , Baldo Vincenzo
Published: July 2022

In December 2021–January 2022 the Veneto region in Italy faced an unprecedented wave of SARS-CoV-2 infections, even though both the vaccine coverage and the number of previously infected individuals keep increasing. In this study we address the protection against the SARS-CoV-2 infection offered by natural immunity and a three-dose regimen through a retrospective study based on Veneto’s regional databases. In particular, we compared these protection levels during two distinct periods respectively representative of the Delta (B.1.617.2) and the Omicron (B.1.1.529) variants, in order to investigate and quantify the immunological evasion, especially of the Omicron. For each period we compared the incidence rate of infection among the population with various immunological protections against SARS-CoV-2 and performed a multivariable proportional hazard Cox binomial regression to assess the effectiveness afforded by both forms of active immunization. We found out that a previous SARS-CoV-2 infection (irrespective of its timing) offers 85% (83–87%) and 36% (33–39%) protection against being reinfected by Delta and Omicron, respectively. In addition, we estimated the third dose to be more effective in both periods and to have a minor proportional loss of effectiveness due to the rise of the Omicron variant, with an afforded effectiveness against SARS-CoV-2 Delta and Omicron infection of 97% (96–97%) and 47% (45–48%), respectively. Our findings suggest that viral variant factors may affect any form of active immunization but that receiving a booster vaccination cycle is more effective and less variable than natural immunity in terms of afforded protection against SARS-CoV-2 infections.
Seroprevalence Rates against West Nile, Usutu, and Tick-Borne Encephalitis Viruses in Blood-Donors from North-Western Romania
Authors: Coroian Mircea , Mihalca Andrei Daniel , Dobler Gerhard , Euringer Kathrin , Girl Philipp , Borșan Silvia-Diana , Kalmár Zsuzsa , Tincuța Briciu Violeta , Flonta Mirela , Topan Adriana , Rădulescu Amanda Lelia , Ungur Andrei , Lupșe Mihaela Sorina
Published: July 2022

Introduction: West Nile virus (WNV), Usutu virus (USUV), and the tick-borne encephalitis virus (TBEV) are all arboviruses belonging to Flaviviridae family. All are characterized by vectorial transmission and sometimes associated with neuroinvasive infections. The circulation of these viruses is considered endemic in parts of Europe, with human cases reported in many countries. Among hosts, the viruses are vectored by hematophagous arthropods, such as mosquitoes (WNV, USUV) and ticks (TBEV). Considering the currently outdated knowledge regarding the epidemiology of these viruses in Romania, the aim of our study was to assess the seroprevalence rates of WNV, USUV, and TBEV among healthy blood donors in north-western Romania. Methods: Human blood samples from healthy donors were collected between November 2019 and February 2020 in six counties from the north-western region of Romania. The samples were serologically tested by ELISA and serum neutralization test. Results: Overall, we obtained a seroprevalence of 3.17% for WNV, 0.08% for TBEV, and 0% for USUV. Conclusion: Despite the low seroprevalence of WNV, USUV, and TBEV in our study, we highlight the need for continuous nationwide vector and disease surveillance and implementation of control measures. Further research is required for an optimal overview of the epidemiological status of the Romanian population regarding these flaviviruses together with countrywide awareness campaigns.
Sedentary Patterns and Sit-to-Stand Transitions in Open Learning Spaces and Conventional Classrooms among Primary School Students
Authors: Hartikainen Jani , Haapala Eero A. , Sääkslahti Arja , Poikkeus Anna-Maija , Finni Taija
Published: July 2022

Educational reforms worldwide have resulted in schools increasingly incorporating open and flexible classroom designs that may provide possibilities to reduce sedentary behavior among students during lessons. Cross-sectional associations of classroom type on accelerometry assessed sedentary bout durations and sit-to-stand transitions were investigated in 191 third and fifth grade students recruited from one school with open learning spaces and two schools with conventional classrooms. A three-way ANOVA for classroom type, gender and grade level indicated that students in open learning spaces had more 1-to-4-min sedentary bouts (mean difference 1.8 bouts/h, p < 0.001), fewer >10-min sedentary bouts (median 0.20 vs. 0.48 bouts/h, p = 0.004) and more sit-to-stand transitions (mean difference 0.9 STS/h, p = 0.009) than students in conventional learning spaces. Comparisons between schools by grade, which were conducted with a one-way ANCOVA adjusted for gender, indicated that most of the significant differences occurred between schools with different classroom types. There were only small and mostly statistically nonsignificant differences between the two schools with conventional classrooms. In conclusion, open learning spaces may improve children’s sedentary profiles towards shorter sedentary bout durations and facilitate also postural transitions during lessons, which may translate into beneficial health impacts over a longer period.
Adjustable Parameters and the Effectiveness of Adjunct Robot-Assisted Gait Training in Individuals with Chronic Stroke
Authors: Chen Shih-Ching , Kang Jiunn-Horng , Peng Chih-Wei , Hsu Chih-Chao , Lin Yen-Nung , Lai Chien-Hung
Published: July 2022

The aims of this study were (1) to compare the effect of robot-assisted gait orthosis (RAGO) plus conventional physiotherapy with the effect of conventional therapy alone on functional outcomes, including balance, walking ability, muscle strength, daily activity, and cognition, in chronic stroke patients, and (2) to determine the association of adjustable parameters of RAGO on functional outcomes. Adjustable parameters of RAGO included guidance force, treadmill speed, and body-weight support. This retrospective cohort study enrolled 32 patients with chronic stroke. Of these, 16 patients received RAGO plus conventional physiotherapy (RAGO group), and 16 patients received conventional physiotherapy alone (control group). Balance was assessed using the Berg Balance Scale, walking ability using the Functional Ambulation Category, muscle strength using the Motricity Index, daily activity using the Barthel Index, and cognition using the Mini-Mental State Examination. The scores were assessed before and after training. The Mini–Mental State Examination and the Berg Balance Scale increased significantly in both groups, whereas improvements in the Motricity Index and the Barthel Index were only observed in the RAGO group after intervention. During RAGO training, reducing guidance force and body-weight support assistance was associated with improvements in the Barthel Index, whereas higher treadmill walking speed was associated with improvements in the Berg Balance Scale. Our study found that RAGO combination therapy resulted in improvements in more functional outcomes than did conventional training alone. The adjustable parameters of the RAGO training were partly associated with training outcomes.
Perceived Quality and Users’ Satisfaction with Public–Private Partnerships in Health Sector
Authors: Carvalho João M. S. , Rodrigues Nuno
Published: July 2022

In Portugal, the government has accepted private management within public hospitals since 1996. The objectives of the state were to ensure more efficiency in resource management and maintain or increase the service quality provided to the users. Four public hospitals have been managed with a public–private partnership (PPP) approach. This study aimed to empirically analyse the degree of satisfaction of the Portuguese population regarding the service quality provided by PPP and Public Management Hospitals (PMH) within a structural equation model, and verify if people’s literacy level, age, education, and income moderate their opinions. The study used 2077 valid questionnaire responses applied in the four regions served by the eight hospitals. The results show that the users of the PPP hospitals are more satisfied than those from PMH with statistical significance. Literacy level moderates the relationship between perceived quality and users’ satisfaction, and education moderates the same relationship only in the context of PPP hospitals. More educated people with a high literacy level are more demanding, both regarding PPP and PMH hospitals. Nevertheless, the results are very beneficial to the PPP model; thus, improved decision-making regarding contract renewal might help policymakers consider the findings of this paper.
Transformational Leadership and Psychological Well-Being of Service-Oriented Staff: Hybrid Data Synthesis Technique
Authors: Kim Hyun-Duck , Cruz Angelita Bautista
Published: July 2022

Leaders play a significant role in organizations and their leadership behaviors can either enhance or undermine the well-being of their employees. This study aimed to meta-analyze the relationship between transformational leadership and well-being in the service industry, and how employees’ gender and service sector moderated the strength of this relationship. This study used a convergent mixed-method approach. PubMed, MEDLINE, Google Scholar, AMED, and Scopus electronic databases were utilized to search for relevant studies. Textual data were analyzed using a text data-mining technique (Leximancer) to determine the relevant themes and concepts. Statistical data were examined through a comprehensive meta-analysis to determine their effect sizes. The qualitative results outline the major themes that emerged: leadership, well-being, and health. The quantitative findings revealed that the perceived well-being of male employees and those working outside of the health-care service sector was positively higher when employees’ leaders showed transformational leadership. In general, the findings from the qualitative and quantitative data converge. The findings confirm the positive relationship between transformational leadership and employee well-being. This study also highlights the applicability of a convergent mixed-method approach as a useful methodological strategy when analyzing both lexical and statistical data.
Workers’ Perception Heat Stress: Results from a Pilot Study Conducted in Italy during the COVID-19 Pandemic in 2020
Authors: Bonafede Michela , Levi Miriam , Pietrafesa Emma , Binazzi Alessandra , Marinaccio Alessandro , Morabito Marco , Pinto Iole , de’ Donato Francesca , Grasso Valentina , Costantini Tiziano , Messeri Alessandro
Published: July 2022

Many workers are exposed to the effects of heat and often to extreme temperatures. Heat stress has been further aggravated during the COVID-19 pandemic by the use of personal protective equipment to prevent SARS-CoV-2 infection. However, workers’ risk perception of heat stress is often low, with negative effects on their health and productivity. The study aims to identify workers’ needs and gaps in knowledge, suggesting the adaptation of measures that best comply with the needs of both workers and employers. A cross-sectional online questionnaire survey was conducted in Italy in the hottest months of 2020 (June–October) through different multimedia channels. The data collected were analyzed using descriptive statistics; analytical tests and analysis of variance were used to evaluate differences between groups of workers. In total, 345 questionnaires were collected and analyzed. The whole sample of respondents declared that heat is an important contributor to productivity loss and 83% of workers did not receive heat warnings from their employer. In this context, the internet is considered as the main source of information about heat-related illness in the workplace. Results highlight the need to increase workers’ perception of heat stress in the workplace to safeguard their health and productivity. About two-thirds of the sample stated that working in the sun without access to shaded areas, working indoors without adequate ventilation, and nearby fire, steam, and hot surfaces, represent the main injuries’ risk factors.
Phosphomimicry on STAU1 Serine 20 Impairs STAU1 Posttranscriptional Functions and Induces Apoptosis in Human Transformed Cells
Authors: Gonzalez Quesada Yulemi , Bonnet-Magnaval Florence , DesGroseillers Luc
Published: July 2022

Staufen 1 (STAU1) is an RNA-binding protein that is essential in untransformed cells. In cancer cells, it is rather STAU1 overexpression that impairs cell proliferation. In this paper, we show that a modest increase in STAU1 expression in cancer cells triggers apoptosis as early as 12 h post-transfection and impairs proliferation in non-apoptotic cells for several days. Interestingly, a mutation that mimics the phosphorylation of STAU1 serine 20 is sufficient to cause these phenotypes, indicating that serine 20 is at the heart of the molecular mechanism leading to apoptosis. Mechanistically, phosphomimicry on serine 20 alters the ability of STAU1 to regulate translation and the decay of STAU1-bound mRNAs, indicating that the posttranscriptional regulation of mRNAs by STAU1 controls the balance between proliferation and apoptosis. Unexpectedly, the expression of RBD2S20D, the N-terminal 88 amino acids with no RNA-binding activity, is sufficient to induce apoptosis via alteration, in trans, of the posttranscriptional functions of endogenous STAU1. These results suggest that STAU1 is a sensor that controls the balance between cell proliferation and apoptosis, and, therefore, may be considered as a novel therapeutic target against cancer.
Impact of Double Covalent Binding of BV in NIR FPs on Their Spectral and Physicochemical Properties
Authors: Stepanenko Olga V. , Kuznetsova Irina M. , Turoverov Konstantin K. , Stepanenko Olesya V.
Published: July 2022

Understanding the photophysical properties and stability of near-infrared fluorescent proteins (NIR FPs) based on bacterial phytochromes is of great importance for the design of efficient fluorescent probes for use in cells and in vivo. Previously, the natural ligand of NIR FPs biliverdin (BV) has been revealed to be capable of covalent binding to the inherent cysteine residue in the PAS domain (Cys15), and to the cysteine residue introduced into the GAF domain (Cys256), as well as simultaneously with these two residues. Here, based on the spectroscopic analysis of several NIR FPs with both cysteine residues in PAS and GAF domains, we show that the covalent binding of BV simultaneously with two domains is the reason for the higher quantum yield of BV fluorescence in these proteins as a result of rigid fixation of the chromophore in their chromophore-binding pocket. We demonstrate that since the attachment sites are located in different regions of the polypeptide chain forming a figure-of-eight knot, their binding to BV leads to shielding of many sites of proteolytic degradation due to additional stabilization of the entire protein structure. This makes NIR FPs with both cysteine residues in PAS and GAF domains less susceptible to cleavage by intracellular proteases.
Adipose Tissue Inflammation and Pulmonary Dysfunction in Obesity
Authors: Palma Giuseppe , Sorice Gian Pio , Genchi Valentina Annamaria , Giordano Fiorella , Caccioppoli Cristina , D’Oria Rossella , Marrano Nicola , Biondi Giuseppina , Giorgino Francesco , Perrini Sebastio
Published: July 2022

Obesity is a chronic disease caused by an excess of adipose tissue that may impair health by altering the functionality of various organs, including the lungs. Excessive deposition of fat in the abdominal area can lead to abnormal positioning of the diaphragm and consequent reduction in lung volume, leading to a heightened demand for ventilation and increased exposure to respiratory diseases, such as chronic obstructive pulmonary disease, asthma, and obstructive sleep apnoea. In addition to mechanical ventilatory constraints, excess fat and ectopic deposition in visceral depots can lead to adipose tissue dysfunction, which promotes metabolic disorders. An altered adipokine-secretion profile from dysfunctional adipose tissue in morbid obesity fosters systemic, low-grade inflammation, impairing pulmonary immune response and promoting airway hyperresponsiveness. A potential target of these adipokines could be the NLRP3 inflammasome, a critical component of the innate immune system, the harmful pro-inflammatory effect of which affects both adipose and lung tissue in obesity. In this review, we will investigate the crosstalk between adipose tissue and the lung in obesity, highlighting the main inflammatory mediators and novel therapeutic targets in preventing pulmonary dysfunction.
The Diabetic Cardiorenal Nexus
Authors: D’Elia John A. , Bayliss George P. , Weinrauch Larry A.
Published: July 2022

The end-stage of the clinical combination of heart failure and kidney disease has become known as cardiorenal syndrome. Adverse consequences related to diabetes, hyperlipidemia, obesity, hypertension and renal impairment on cardiovascular function, morbidity and mortality are well known. Guidelines for the treatment of these risk factors have led to the improved prognosis of patients with coronary artery disease and reduced ejection fraction. Heart failure hospital admissions and readmission often occur, however, in the presence of metabolic, renal dysfunction and relatively preserved systolic function. In this domain, few advances have been described. Diabetes, kidney and cardiac dysfunction act synergistically to magnify healthcare costs. Current therapy relies on improving hemodynamic factors destructive to both the heart and kidney. We consider that additional hemodynamic solutions may be limited without the use of animal models focusing on the cardiomyocyte, nephron and extracellular matrices. We review herein potential common pathophysiologic targets for treatment to prevent and ameliorate this syndrome.
Macro- and Nanoscale Effect of Ethanol on Bovine Serum Albumin Gelation and Naproxen Release
Authors: Sanaeifar Niuosha , Mäder Karsten , Hinderberger Dariush
Published: July 2022

We report extended ethanol-induced gelation procedures of bovine serum albumin (BSA) at 37 °C and investigate the release behavior of a spin-labeled naproxen derivative (SL-NPX) from these hydrogels. The macroscopic mechanical properties of these gels during formation were studied using rheology, while a nanoscopic, more molecular view was obtained by analyzing the secondary structure of the protein during gelation via infrared (ATR-IR) spectroscopy. To evaluate the potential use of BSA hydrogels in controlled drug delivery, SL-NPX-BSA interaction was investigated in detail by continuous-wave electron paramagnetic resonance (CW EPR) spectroscopy, which provides information on the interaction of the small drug molecules and the hydrogel. In addition to CW EPR spectroscopy, dynamic light scattering (DLS), which provides insight into the size and nature of released components, was applied to characterize the combined influence of incubation time, ethanol, SL-drug, and BSA concentration on release behavior. It was found that the alteration of initial drug loading percentage, hydrogel incubation time as well as BSA and alcohol concentrations affect and thus tune the release rate of SL-NPX from BSA hydrogels. These results lead to the conclusion that BSA hydrogels as controlled release systems offer a remarkable fine-tuning capability for pharmaceutical applications due to the variety of gelation parameters.