bluuebunny/crossref_metadata_2025 huggingface dataset:
Clonal and Scalable Endothelial Progenitor Cell Lines from Human Pluripotent Stem Cells
Authors: Lee Jieun , Sternberg Hal , Bignone Paola A. , Murai James , Malik Nafees N. , West Michael D. , Larocca Dana
Published: October 2023

Human pluripotent stem cells (hPSCs) can be used as a renewable source of endothelial cells for treating cardiovascular disease and other ischemic conditions. Here, we present the derivation and characterization of a panel of distinct clonal embryonic endothelial progenitor cells (eEPCs) lines that were differentiated from human embryonic stem cells (hESCs). The hESC line, ESI-017, was first partially differentiated to produce candidate cultures from which eEPCs were cloned. Endothelial cell identity was assessed by transcriptomic analysis, cell surface marker expression, immunocytochemical marker analysis, and functional analysis of cells and exosomes using vascular network forming assays. The transcriptome of the eEPC lines was compared to various adult endothelial lines as well as various non-endothelial cells including both adult and embryonic origins. This resulted in a variety of distinct cell lines with functional properties of endothelial cells and strong transcriptomic similarity to adult endothelial primary cell lines. The eEPC lines, however, were distinguished from adult endothelium by their novel pattern of embryonic gene expression. We demonstrated eEPC line scalability of up to 80 population doublings (pd) and stable long-term expansion of over 50 pd with stable angiogenic properties at late passage. Taken together, these data support the finding that hESC-derived clonal eEPC lines are a potential source of scalable therapeutic cells and cell products for treating cardiovascular disease. These eEPC lines offer a highly promising resource for the development of further preclinical studies aimed at therapeutic interventions.
Behavioral Effects and Analgesic Profile of Hemoglobin-Derived Valorphin and Its Synthetic Analog in Rodents
Authors: Todorov Petar , Assenov Borislav , Angelov Dimo , Dzhambazova Elena , Pechlivanova Daniela
Published: October 2023

Valorphin (V1) is a naturally occurring peptide derived from hemoglobin that has been found to have an affinity for opioid receptors and exhibits antinociceptive and anticonvulsant activity. Some of its synthetic analogs containing an aminophosphonate moiety show structure-dependent potent antinociceptive effects. This study aimed to reveal a detailed picture of the antinociceptive mechanisms and behavioral effects of V1 and its recently synthesized phosphopeptide analog V2p in rodents using a range of methods. The studied peptides significantly reduced acute (mean V1–9.0, V2p–5.8 vs. controls–54.1 s) and inflammatory (mean V1–57.9 and V2p–53.3 vs. controls–107.6 s) nociceptive pain in the formalin test, as well as carrageenan-induced hyperalgesia (mean V1–184.7 and V2p–107.3 vs. controls–61.8 g) in the paw pressure test. These effects are mediated by activation of opioid receptors with a predominance of kappa in V1 antinociception and by delta, kappa, and mu receptors in V2p-induced antinociception. Both peptides did not change the levels of TNF-alpha and IL-1-beta in blood serum. V1 induces depression-like behavior, and V2p shows a tendency toward anxiolysis and short-term impairment of motor coordination without affecting exploratory behavior. The results characterize valorphin and its derivative as promising analgesics that exert their effects both centrally and peripherally, without causing severe behavioral changes in experimental animals. These encouraging data are a foundation for future studies focusing on the effects of hemorphins after long-term treatment.
Differences and Changes in Cerebellar Functional Connectivity of Parkinson’s Patients with Visual Hallucinations
Authors: Qu Liangcheng , Liu Chuan , Cao Yiting , Shi Jingping , Yin Kuiying , Liu Weiguo
Published: October 2023

Recent studies have discovered that functional connections are impaired in patients with Parkinson’s disease (PD) accompanied by hallucinations (PD-H), even at the preclinical stage. The cerebellum has been implicated in playing a role in cognitive processes. However, the functional connectivity (FC) between the cognitive sub-regions of the cerebellum in PD patients with hallucinations needs further clarification. Resting-state functional magnetic resonance imaging (rs-fMRI) data were collected from three groups (17 PD-H patients, 13 patients with Parkinson’s disease not accompanied by hallucinations (PD-NH), and 26 healthy controls (HC)). The data were collected in this study to investigate the impact of cerebellar FC changes on cognitive performance. Additionally, we define cerebellar FC as a training feature for classifying all subjects using Support Vector Machines (SVMs). We found that in the PD-H patients, there was an increase in FC within the left side of the precuneus (PCUN) compared to the HC. Additionally, there was an increase in FC within the bilateral opercular part of the inferior frontal gyrus (IFGoprec) and triangular part of the inferior frontal gyrus (IFCtriang), as well as the left side of the postcentral gyrus (PoCG), inferior parietal lobe (IPL), and PCUN compared to the PD-NH patients. In the machine learning training results, cerebellar FC has also been proven to be an effective biomarker feature, achieving a recognition rate of over 90% for PD-H. These findings indicate that the cortico-cerebellar FC in PD-H and PD-NH patients was significantly disrupted, with different patterns of distribution. The proposed pipeline offers a promising, low-cost alternative for diagnosing preclinical PD-H and may also be beneficial for other degenerative brain disorders.
GPR4 Knockout Attenuates Intestinal Inflammation and Forestalls the Development of Colitis-Associated Colorectal Cancer in Murine Models
Authors: Marie Mona A. , Sanderlin Edward J. , Hoffman Alexander P. , Cashwell Kylie D. , Satturwar Swati , Hong Heng , Sun Ying , Yang Li V.
Published: October 2023

GPR4 is a proton-sensing G protein-coupled receptor highly expressed in vascular endothelial cells and has been shown to potentiate intestinal inflammation in murine colitis models. Herein, we evaluated the proinflammatory role of GPR4 in the development of colitis-associated colorectal cancer (CAC) using the dextran sulfate sodium (DSS) and azoxymethane (AOM) mouse models in wild-type and GPR4 knockout mice. We found that GPR4 contributed to chronic intestinal inflammation and heightened DSS/AOM-induced intestinal tumor burden. Tumor blood vessel density was markedly reduced in mice deficient in GPR4, which correlated with increased tumor necrosis and reduced tumor cell proliferation. These data demonstrate that GPR4 ablation alleviates intestinal inflammation and reduces tumor angiogenesis, development, and progression in the AOM/DSS mouse model.
Deletions of CDKN2A and MTAP Detected by Copy-Number Variation Array Are Associated with Loss of p16 and MTAP Protein in Pleural Mesothelioma
Authors: Vrugt Bart , Kirschner Michaela B. , Meerang Mayura , Oehl Kathrin , Wagner Ulrich , Soltermann Alex , Moch Holger , Opitz Isabelle , Wild Peter J.
Published: October 2023

CDKN2A deletion is a common alteration in pleural mesothelioma (PM) and frequently associated with co-deletion of MTAP. Since the standard detection method for CDKN2A deletion and FISH analysis is relatively expensive, we here investigated the suitability of inexpensive p16 and MTAP IHC by comparing concordance between IHC and OncoScan CNV arrays on samples from 52 PM patients. Concordance was determined using Cohen’s kappa statistics. Loss of CDKN2A was associated with co-deletion of MTAP in 71% of cases. CDKN2A-MTAP copy-number normal cases were also IHC positive in 93% of cases for p16 and 100% for MTAP, while homozygous deletion of CDKN2A-MTAP was always associated with negative IHC for both proteins. In cases with heterozygous CDKN2A-MTAP loss, IHC expression of p16 and MTAP was negative in 100% and 71%, respectively. MTAP and p16 IHC showed high sensitivity (MTAP 86.5%, p16 100%) and specificity (MTAP 100%, p16 93.3%) for the detection of any gene loss. Loss of MTAP expression occurred exclusively in conjunction with loss of p16 labeling. Both p16 and MTAP IHC showed high concordance with Oncoscan CNV arrays (kappa = 0.952, p < 0.0001, and kappa = 0.787, p < 0.0001 respectively). We recommend combined MTAP and p16 immunohistochemistry to confirm the diagnosis of PM.
Accelerated Aging and the Life Course of Individuals Born Preterm
Authors: Bousquet Audrey , Sanderson Keia , O’Shea T. Michael , Fry Rebecca C.
Published: October 2023

Individuals born preterm have shorter lifespans and elevated rates of chronic illness that contribute to mortality risk when compared to individuals born at term. Emerging evidence suggests that individuals born preterm or of low birthweight also exhibit physiologic and cellular biomarkers of accelerated aging. It is unclear whether, and to what extent, accelerated aging contributes to a higher risk of chronic illness and mortality among individuals born preterm. Here, we review accelerated aging phenotypes in adults born preterm and biological pathways that appear to contribute to accelerated aging. We highlight biomarkers of accelerated aging and various resiliency factors, including both pharmacologic and non-pharmacologic factors, that might buffer the propensity for accelerated aging among individuals born preterm.
Clinical and Genetic Characterization of Patients with Primary Ciliary Dyskinesia in Southwest Saudi Arabia: A Cross Sectional Study
Authors: Asseri Ali Alsuheel , Shati Ayed A. , Asiri Ibrahim A. , Aldosari Reem H. , Al-Amri Hassan A. , Alshahrani Mohammed , Al-Asmari Badriah G. , Alalkami Haleimah
Published: October 2023

Background: Primary ciliary dyskinesia (PCD, MIM 244400) is an inherited ciliopathy disorder characterized by recurrent sinopulmonary infections, subfertility, and laterality defects. The true incidence of PCD in Saudi Arabia is not known, but it is likely underdiagnosed due to the high prevalence of consanguineous marriages. In this study, we aim to study the clinical and genetic characteristics of PCD patients in the southwestern region of Saudi Arabia to provide guidance to clinicians and researchers studying PCD. Methods: This was a cross-sectional study conducted between 2019 and 2023 in Abha Maternity and Children’s Hospital. Twenty-eight patients with clinically diagnosed PCD were recruited. The diagnosis of PCD was confirmed via whole-exome sequencing. Results: A total of 28 patients from 20 families were identified and recruited for this study. The median age of patients was 7.5 years (IQR = 3, 13 years). The people of different sexes were evenly distributed, and 18 patients (64%) had neonatal respiratory distress (NRD). The median age of diagnosis was 5.5 years (IQR = 2, 11 years), while the age when the first symptoms appeared was 3 months old (IQR = 1, 6 months). The prevalence of a chronic wet cough, chronic rhinosinusitis, ear infections were 100% (n = 28), 78.6% (n = 22), and 67.9% (19), respectively. The most common gene in our study was DNAH5, which represented 17.9% (five out of twenty-eight) of the cases. Furthermore, the remaining pathogenic variants included: 14.3% with RSPH9 in four individuals (three families), 14.3% with DNAI2 in four individuals (two families), and 10.7% with LRRC56 in three individuals (one family). The most common findings on the chest CT scans were consolidation (seen in all patients), mucus plugging (seen in 95%), and bronchiectasis (seen in 77%). In the patients with bronchiectasis, the most commonly affected lobes were the right lower lobe (88%) and left lower lobe (76%). The patients with PCD and situs inversus were more likely to experience NRD than the patients with PCD and situs solitus. The median PICADAR score in the patients with PCD and situs inversus (median: 11.5; Q1: 10–Q3: 12.5) was significantly higher compared to those with PCD and situs solitus (median: 7.5; Q1: 5.8–Q3: 8) (U = 10.5; p < 0.001). Conclusion: This study provides preliminary data on the clinical and genetic characteristics of PCD patients in the southwestern region of Saudi Arabia. We found that DNAH5 and RSPH9 genes were the most common genes among the studied population. Furthermore, PCD should be considered for each child with early NRD and laterality defects, and further confirmatory tests are recommended. These findings also highlight the need for greater awareness of the disease in daily clinical practice to facilitate early diagnosis and avoid irreversible lung damage.
Assessing the Sustainable Potential of Corrugated Board-Based Bundle Packaging of PET Bottles: A Life Cycle Perspective—A Case Study
Authors: Jannes Roman , Vanhauwermeiren Pieter , Slaets Peter , Juwet Marc
Published: October 2023

Large amounts of PET bottles are used worldwide as primary packaging for numerous liquids, including water and soft drinks. In many cases, between two and eight of such bottles are bundled for transport and sales using plastic collation shrink film. This study evaluates paper-based alternatives for plastic bundling material using a life cycle assessment (LCA) of four different types of bundle packaging: LDPE shrink film, recycled Low-Density Polyethylene (rLDPE) shrink film, Ecogrip (existing paper-based bundle packaging) and Ecobundle (new paper-based bundle packaging). The study focuses on the case of bundling six bottles of 1.5 L of sparkling water. The most environmentally friendly option is identified, taking into consideration the material usage, effects on human health and effects on the biosphere. It is concluded that the new corrugated board-based bundling method (Ecobundle) is very promising. Further optimization of the design, minimization of material, improved choice of materials and improved design of the production machinery may result in a corrugated board performing even better than rLDPE shrink film in terms of the global warming potential.
Effect of Thickness of Ti Coating Deposited by Vacuum Arc Melting on Fatigue Behavior of Aluminum Alloy Al–5%Si
Authors: Zaguliaev Dmitrii , Ivanov Yurii , Gudala Suresh , Tolkachev Oleg , Aksenova Krestina , Konovalov Sergey , Shlyarov Vitaly
Published: October 2023

Fatigue strength tests of Ti-coated aluminum alloys with a thickness of 1 µm, 3 µm, and 5 µm were conducted to investigate the effect of the coating thickness on fatigue strength. Under the same applied stress amplitude, the optimum thickness with the most-extended fatigue life was around the coating thickness of 5 µm. This may be attributed to the good resistance to surface cracks under repeated loads. The results suggested that a lower fatigue life of a coating thickness of 1 µm results from the fracture of the coating layer under the strong influence of the deformation of the substrate. This could be due to the higher tensile residual stress induced in the substrate near the coating layer and substrate interface. The titanium coating restricted the initiation of offsets and cracks beneath the surface of the specimen, which may be attributed to the high strength of the Al–5%Si substrate, good flexibility, and strong adhesion, which provided sufficient compressive stress to suppress slip band protrusions. The fatigue life and fatigue limit increased proportionally to the thickness of the titanium coating due to changes in the surface roughness and adhesion capability.
Wear Resistance Study of Bionic Pitted Ni Cladding Layer on 7075 Aluminum Alloy Drill Pipe Surface
Authors: Li Xu , Gao Ke , Zhao Yan , Xie Xiaobo , Lü Xiaoshu , Zhang Cong , Ai Hongxin
Published: October 2023

To enhance the lifespan of drill pipes and minimize wear, this study introduces a bionic structure model inspired by the pit shape structure found in the dung beetle’s abdomen. The stress distribution and wear of bionic pitted structure and ordinary structure are simulated by finite element software. The findings revealed that the bionic structure significantly improves stress distribution, resulting in an impressive 81.3% increase in lifespan. Subsequently, the surface of the 7075 aluminum drill pipe was coated with Ni powder by a laser cladding system. Wear tests were conducted to analyze the wear and surface damage behavior of the cladding layer. The microstructure, composition, and microhardness of the cladding layer were measured and observed. The results showed that the cladding layer was mainly composed of Al3Ni2 and had high hardness. Additionally, a transition region exists between the cladding layer and the substrate, comprising relatively low hardness Al, thereby enhancing the drill pipe’s ability to withstand alternating loads. Furthermore, the bionic structure possesses the capability to store particles, effectively reducing the occurrence of abrasive wear and increasing the lifespan by 70.0%.
A Review of Carbon-Based Conductive Inks and Their Printing Technologies for Integrated Circuits
Authors: Qin Yufeng , Ouyang Xueqiong , Lv Yang , Liu Wencai , Liu Qing , Wang Shuangxi
Published: October 2023

In recent years, researchers prepared composite conductive inks with high conductivity, high thermal conductivity, strong stability, and excellent comprehensive mechanical properties by combining carbon-based materials such as graphene and carbon nanotubes with metal-based materials. Through new electronic printing technologies, conductive inks can be used not only to promote the development of integrated circuits but also in various new electronic products. The conductive mechanism and the main types of conductive inks are introduced in this review. The advantages of electronic printing technology for preparing integrated circuits are analyzed. The research progress of fabricating integrated circuits with different electronic printing processes, such as screen printing, gravure printing, flexographic printing, and inkjet printing, are summarized. The development trend of carbon-based composite conductive ink for integrated circuits is prospected.
The Swiss Narcolepsy Network (SNaNe)
Authors: Bassetti Claudio L. A. , Khatami Ramin , Miano Silvia , Wenz Elena , Werth Esther
Published: October 2023

The Swiss Narcolepsy Network (SNaNe) was founded in 2017 as a non-profit organization with the vision of improving the care of patients with narcolepsy, central disorders of hypersomnolence (CDH), and rare sleep disorders. The SNaNe aims at maximizing the speed of diagnosis, minimizing difficulties stemming from the rare nature of these conditions, and providing patients with optimum health care throughout the course of their disease. In addition, the SNaNe promotes education, awareness, and research on CDH and rare sleep disorders. The article reports the current structure, organization, and the following main activities of the SNaNe: (1) the discussion of complex patient cases; (2) the organization of the Swiss Narcolepsy Days; (3) the coordination of multicenter research projects (e.g., SPHYNCS and iSPHYNCS studies); (4) the establishment of an anonymous Swiss registry for CDH patients (SNaNe Data Registry); (5) the collaboration with the national patients’ organization (SNAG); and (6) the collaboration with other national and international scientific, professional, and patients’ (eNAP) organizations.
Impact of Output Gap, COVID-19, and Governance Quality on Fiscal Space in Sub-Saharan Africa
Authors: Katuka Blessing , Mudzingiri Calvin
Published: October 2023

This study examined the determinants of fiscal space within the Sub-Saharan Africa (SSA) region, utilising a panel of 33 countries from 2005 to 2021. The paper applied the panel threshold, difference, and system generalised method of moments (GMM) regression techniques. The empirical results found evidence of constrained fiscal space and poor governance in Central, Western, and Eastern Africa. The results further unveiled that an enhancement in governance indicators beyond −0.23 for the governance index, −0.15 for control of corruption, −0.98 for the rule of law, −0.37 for regulatory quality, −0.15 for voice and accountability, +0.36 for political stability, and −0.61 for government effectiveness, respectively, increase fiscal space. Moreover, the study concluded that the output gap, COVID-19, trade openness, and economic growth impact fiscal space availability in Central, Western, Southern, and Eastern Africa. The paper investigated whether the COVID-19 pandemic and governance quality significantly influenced fiscal space within SSA. We strongly recommend enhancement in all facets of governance through comprehensive restructuring of governance policies across all SSA countries. Another key recommendation is fostering trade openness to expand tax revenue generation and broaden the tax base, thereby providing the continent with greater fiscal space and improved resilience to unforeseen shocks.
Digital Technology and Teacher Professional Development: Challenges and Contradictions in Compulsory Education
Authors: Montero-Mesa Lourdes , Fraga-Varela Fernando , Vila-Couñago Esther , Rodríguez-Groba Ana
Published: October 2023

The aim of this article is to explore the role of teacher professional development (TPD) in relation to two experiences involving digital technology. Both studies were conducted at a single combined primary and secondary school and involved a total of 727 students. The first study consisted of an ex post facto analysis of attempts to improve student perception of the risks associated with inappropriate use of technology, while the second examined the possible benefits of gamified learning on mathematical fluency using a pre-test/post-test approach. The results reveal the lack of TPD in relation to both experiences, with teachers unable to overcome problems related to technology use and risk perception in the first case, and relegated to the role of mere implementers of an automated learning software product in the second. The conclusions highlight the contradiction between the image of technological innovation in schools and the reality of stasis in teaching practice, as well as the need for further research to assess and promote more effective TPD.
A Bibliometric Analysis of the Research Progress and Trends during 2002–2022 on the Carbon Stocks in Terrestrial Ecosystems
Authors: Sawirdin Subinur , Tian Aolei , Shi Lei , Fu Wentao , Cheng Shengyuan , Halik Ümüt , Liang Jiye
Published: October 2023

Improving the carbon storage in terrestrial ecosystems can effectively reduce atmospheric CO2, which is one of the important ways of mitigating global climate change. The knowledge on terrestrial carbon stock research is relatively mature in the international community; however, the research pulse, hotspots, and trends in terrestrial ecosystems carbon stock research are not clear. To better understand the research focus and developmental directions of terrestrial ecosystems’ carbon storage, we conducted a bibliometric analysis of 6305 research articles between 2002 and 2022, which were gathered from the Web of Science Core Database. The temporal distribution, country/region distribution, co-citation network, keyword evolution and clustering, journals, and authors of the literature were analyzed, and the knowledge domain was mapped using the CiteSpace visualization software. This study established the following three observations: (1) The number of publications on carbon stock research in terrestrial ecosystems continues to expand, and the trend in the number of publications proves that carbon sinks in terrestrial systems remain an important research topic internationally. (2) Important issues concerning terrestrial ecosystem carbon stock research have evolved from monitoring the carbon stock changes in terrestrial ecosystems to the mechanism of carbon stock formation for the realization of monitoring and management under global climate change. Furthermore, the research methodology has evolved from small-scale, fixed-point instrumental observations to large-scale remote sensing and model simulations, with diversifications in research content and methodology. (3) In the future, academic research on carbon stocks in terrestrial ecosystems will focus more on international and interdisciplinary cooperations, increasing the intensity in surveying and monitoring carbon stocks in terrestrial ecosystems, and realizing the accurate monitoring, assessment, management, and enhancement of carbon stocks from the integrated carbon stock computation system for “sky, air, and land.” The results of this study comprehensively demonstrate the current status and development of the research on carbon stocks in terrestrial ecosystems worldwide, and provide a reference for future research on the sustainable management of terrestrial ecosystems within the context of global warming.
Application of LiDAR Derived Fuel Cells to Wildfire Modeling at Laboratory Scale
Authors: Marcozzi Anthony A. , Johnson Jesse V. , Parsons Russell A. , Flanary Sarah J. , Seielstad Carl A. , Downs Jacob Z.
Published: October 2023

Terrestrial LiDAR scans (TLS) offer a rich data source for high-fidelity vegetation characterization, addressing the limitations of traditional fuel sampling methods by capturing spatially explicit distributions that have a significant impact on fire behavior. However, large volumes of complex, high resolution data are difficult to use directly in wildland fire models. In this study, we introduce a novel method that employs a voxelization technique to convert high-resolution TLS data into fine-grained reference voxels, which are subsequently aggregated into lower-fidelity fuel cells for integration into physics-based fire models. This methodology aims to transform the complexity of TLS data into a format amenable for integration into wildland fire models, while retaining essential information about the spatial distribution of vegetation. We evaluate our approach by comparing a range of aggregate geometries in simulated burns to laboratory measurements. The results show insensitivity to fuel cell geometry at fine resolutions (2–8 cm), but we observe deviations in model behavior at the coarsest resolutions considered (16 cm). Our findings highlight the importance of capturing the fine scale spatial continuity present in heterogeneous tree canopies in order to accurately simulate fire behavior in coupled fire-atmosphere models. To the best of our knowledge, this is the first study to examine the use of TLS data to inform fuel inputs to a physics based model at a laboratory scale.
The Riddle of How Fisheries Influence Genetic Diversity
Authors: Sadler Daniel E. , Watts Phillip C. , Uusi-Heikkilä Silva
Published: October 2023

Overfishing drives population decline, which in turn drives loss of genetic diversity. Many studies provide evidence of declines in genetic diversity; however, controversy exists within the literature, as some studies show evidence of no change in genetic diversity despite decades of overharvesting. The apparent discrepancy in the literature should therefore be examined to understand what biological and ecological processes are driving the differences in results. Here, we assess how different factors contribute to fisheries-induced susceptibility to declines in genetic diversity by first focusing on the different roles of genetic markers. Second, we assess how habitat type and conditions contribute to loss of genetic diversity. Third, we assess how life history and physiology affects catchability and loss of genetic diversity. Finally, we discuss how coinciding abiotic and biotic factors influence the intensity of genetic loss. We find a multitude of these factors could be interacting to influence how results are perceived and how intense the loss of genetic diversity can be. Future studies should carefully consider the methodology of genetic analysis used, as well as considerations of life history and ecology of the target species.
Development of Film-Forming Gel Formulations Containing Royal Jelly and Honey Aromatic Water for Cosmetic Applications
Authors: Thewanjutiwong Sirawut , Phokasem Patcharin , Disayathanoowat Terd , Juntrapirom Saranya , Kanjanakawinkul Watchara , Chaiyana Wantida
Published: October 2023

This study aimed to develop a film-forming gel containing honey aromatic water (HW) and royal jelly (RJ) for cosmetic applications as a facial peel-off mask. HW, which is industrial waste from the water-reduction process of honey, was sterilized by autoclaving and filtration through a 0.22 µm membrane. The film-forming gels were developed using various types of film-forming polymers, including polyvinyl alcohol (PVA 117), carboxymethyl cellulose (CMC), and hydroxyethyl cellulose (HEC). The gel formulations were characterized in terms of their external appearance, viscosity, pH, and drying time, whereas the films generated were characterized by a texture analyzer, microscopic investigation, Fourier transform infrared, and an X-ray diffractometer. The findings highlighted that HW has short storage shelf life due to microbial contamination. Sterilizations were required before further product development. The film-forming gel was created by using the combination of PVA 117, CMC, and HEC. HW and RJ were successfully incorporated into the film-forming gel. However, HW resulted in a decrease in the gel viscosity and mechanical properties of its film. Interestingly, the drying time was dramatically decreased, which would be more desirable for its use as a peel-off mask. Furthermore, incorporation of royal jelly enhanced the viscosity of the gels as well as improved the mechanical properties of the film. No effect on the chemical and crystal structure of the films was detected after the incorporation. Therefore, the film-forming gels containing HW and RJ, possessing aesthetic attributes that extended to both the gels themselves and the resultant films, were suitable for use as a peel-off mask.
Migrants and Healthcare during COVID-19, the Case of Kanchanaburi Province in Thailand
Authors: Langkulsen Uma , Mareke Portia , Lambonmung Augustine
Published: October 2023

Since the outbreak of COVID-19, as reported by the WHO in December 2019 and subsequently declared a public health emergency of international concern, a distinct set of risk factors and vulnerabilities faced by migrants are affecting their exposure to the pandemic and its associated outcomes. This study aims to analyze the social determinants of health among migrants and their associated factors and compare the socio-demographic characteristics, patterns of COVID-19, and healthcare attendance and utilization among migrant workers and non-migrants. A descriptive study design was used to analyze COVID-19 morbidity among migrant workers. There were a total of 73,762 migrants living in the province by December 2021, with varied statuses and nationalities. Most of the migrants were from Myanmar, constituting about 80.1%. A total of 24,837 COVID-19 cases in Kanchanaburi province were recorded in 2020–2021. COVID-19 cases among migrant workers accounted for 22.3% during the period under review. Half, 2914 (52.7%) of the migrant female workers were victims of COVID-19 infections. Persons under the age of 18 accounted for about one-fifth of all the COVID-19 cases. Older, over 60 years old, Thais had about twice (10.1%) the COVID-19 cases compared with the older migrants (5.5%). There was a significant increase in healthcare attendance and utilization by non-migrants and migrants during the year under review. Migrants are at high risk of COVID-19 infection. Therefore, public health guidance for the prevention of COVID-19 should prioritize safeguarding the health of migrants by considering their individual characteristics and actions. Enhancing health insurance schemes for migrants, particularly vulnerable migrant groups, is critical for inclusive and expanded healthcare access. Physical, social, and economic environments that impact the health and well-being of migrants should be integral to pandemic prevention, preparedness, response, and recovery.
A Push to Consider Mantle Cell Lymphoma in Adults with Leukemia/Lymphoma with Blastoid Morphology
Authors: Arinze Nkechi , Omar Nivin , Keruakous Amany , Kolhe Ravindra , Savage Natasha
Published: October 2023

Mantle cell lymphoma (MCL) is an intermediate-grade B-cell lymphoma, representing 2.8% of all non-Hodgkin lymphomas in the US. It is associated with t(11;14)(q13; q23), which leads to the overexpression of cyclin D1, consequently promoting cell proliferation. MCL usually expresses CD19, CD20, CD43, surface immunoglobulins, FMC7, BCL2, cyclin D1, CD5, and SOX11. Herein is a case of a 67-year-old male, referred to our facility with shortness of breath, anemia (hemoglobin of 5.3 g/dL), thrombocytopenia (12 × 109/L), and leukocytosis (283 × 109/L). A peripheral blood smear showed marked lymphocytosis with blastoid morphology. Morphologic examination of the bone marrow biopsy revealed a diffuse sheet of blastoid cells expressing CD20 and CD10, but without CD5 or cyclin D1. Given these features, a differential diagnosis of diffuse large B-cell lymphoma (DLBCL) with germinal center derivation, high-grade follicular lymphoma, and Burkitt lymphoma was considered, with the latter not favored due to morphology. Additional studies revealed positive SOX11, and fluorescence in situ hybridization (FISH) studies detected t(11;14). These additional studies supported diagnosis of the blastoid variant of MCL. In conclusion, we present a unique and challenging case of MCL without cyclin D1 or CD5, but with an expression of CD10 and SOX11, along with t(11;14). Pathologists should explicitly consider the blastoid variant of MCL when dealing with mature B-cell neoplasms with blastoid morphology in adults, and utilize a broad panel of ancillary studies, including FISH and SOX11.
Recent Advances and Challenges in Management of Colletotrichum orbiculare, the Causal Agent of Watermelon Anthracnose
Authors: Patel Takshay , Quesada-Ocampo Lina M. , Wehner Todd C. , Bhatta Bed Prakash , Correa Edgar , Malla Subas
Published: October 2023

The fungus Colletotrichum orbiculare causes watermelon anthracnose and is an important pathogen of watermelon in the United States, causing a significant impact on yield and quality of the produce. The application of fungicides as preventative and post-occurrence control measures is currently being deployed by growers. Further study of the genetic and molecular basis of anthracnose resistance will help in guiding future watermelon breeding strategies. Several conserved virulence factors (effectors) in C. orbiculare have been reported to interact with the host, at times impairing the host immune machinery. A single dominant gene conferring race 1 anthracnose resistance was reported independently on two watermelon germplasm. The recent advances in genomics, transcriptomics, proteomics, and metabolomics could facilitate a better understanding of the interaction between C. orbiculare effectors and host resistance genes in the already sequenced watermelon genome. In this review, we encompass and discuss (i) the history of watermelon anthracnose, taxonomy, morphology, and diversity in races of C. orbiculare; (ii) the epidemiology of the anthracnose disease and host resistance; (iii) the genetics behind the pathogenesis; and (iv) the current advances in breeding and molecular efforts to elucidate anthracnose resistance.
Adjoint-Based Design Optimization of a Volute for a Radial Compressor
Authors: Hottois Romain , Châtel Arnaud , Verstraete Tom
Published: October 2023

Numerical optimization methods are widely used for designing turbomachinery components due to the cost and time savings they can provide. In the available literature, the shape optimization of radial compressors mainly focuses on improving the impeller alone. However, it is well-established knowledge that the volute plays a key role in the overall performance of the compressor. The aim of the present paper is to perform an adjoint-based optimization of a volute that is designed for the SRV2-O compressor. The CAD model was first created by using the parametrization of 33 design parameters. Then, a butterfly topology was applied to mesh the computational domain with a multi-block structured grid, and an elliptic smoothing procedure was used to improve the quality of the fluid grid. A steady-state RANS CFD solver with a Spalart-Allmaras turbulence model was used to solve the Navier–Stokes equations, and then the flow sensitivities were computed with an adjoint solver. The objective function consists of minimizing the loss coefficient of the volute. The optimization is performed to obtain an improved design with a 14% loss reduction. A detailed flow and design analysis is carried out to highlight the loss reduction mechanisms, followed by the optimizer. Finally, the compressor map of the full stage is compared between the baseline and the optimized volute from the CFD simulations using a mixing plane interface. This research demonstrates the successful use of a gradient-based optimization technique to improve the volute of a radial compressor and opens the door towards simultaneously optimizing the wheel and the volute.
Installation and Use of a Pavement Monitoring System Based on Fibre Bragg Grating Optical Sensors
Authors: Rebelo Francisco J. P. , Oliveira Joel R. M. , Silva Hugo M. R. D. , Sá Jorge Oliveira e , Marecos Vânia , Afonso João
Published: October 2023

The evolution of technological tools, namely affordable sensors for data collection, and the growing concerns about maintaining roads in adequate conditions have promoted the development of continuous pavement monitoring systems. This paper presents the installation and use of an innovative pavement monitoring system, which was developed to measure the effects of vehicle loads and temperature on the performance of a pavement structure. The sensors used are based on fibre Bragg grating optical technology, collecting data about the strains imposed in the pavement and the temperature at which those measurements are made. The site selection for the system’s installation and the essential installation details to ensure successful data collection are addressed. A calibration procedure was implemented by performing falling weight deflectometer tests and passing preweighed heavy vehicles over the sensors. In addition to validating the system installation, the results obtained in the calibration confirmed the importance of adequately choosing the distance between sensors. Differences of 50 mm in the position of the load may cause differences of about 20% to 25% in the resulting strains. These results confirmed the importance of increasing the sensor concentration in wheel paths. Furthermore, for loads between 25 kN and 65 kN, raising the temperature by 8 °C caused an increase of about 20% in the horizontal tensile strains measured in the pavement. In summary, it was possible to conclude that this innovative system is capable of capturing the effects of temperature and vehicle speed on the response of the pavement, which may be considered an advantage of this type of monitoring system when compared to those that are only used to determine the loads applied to the pavement or to characterise the type of vehicle.
Contemplation of Improvement Efforts to Manage Combined Sewer Overflows
Authors: Jung Younghan Edwin , Jeong M. Myung , Jun Hwandon , Smith Trevor
Published: October 2023

Combined sewer overflow (CSO) is a significant environmental concern and public health risk (e.g., water contamination, eutrophication, and beach closure). The Environmental Protection Agency (EPA) has introduced the National Pollutant Discharge Elimination System (NPDES) permitting program to regulate and address this matter. This program mandates the control of CSOs for more than 700 municipalities obligated to devise Long-term Control Plans (LTCPs) to curb combined sewer overflows and reduce them to safe levels. The LTCP involves diverse strategies, including sewer separation, green infrastructure improvements, and conventional gray infrastructure upgrades. This study investigates several municipalities’ solutions for CSO problems that use conventional methods and wireless sensor technology as real-time control, mainly focusing on a comparative analysis of two cities, Richmond, Virginia, and South Bend, Indiana, such as their average rainfall, the frequency of overflows, and the capacity of treatment plants. The findings indicate that integrating sensor technology could significantly enhance modeling endeavors, bolster the capacity of existing structures, and substantially enhance preparedness for storm events. The EPA’s Storm Water Management Modeling (SWMM) software is utilized. Through an analysis of SWMM data, the study suggests the potential for leveraging wireless sensor technology to achieve more robust control over CSOs and significant cost savings as a part of LTCPs.
Highly Wrinkled Porous Polypyrrole for the Enhancement of the Performance of an Fe2S3-Fe2O3/poly-O-amino Benzenethiol Supercapacitor from a Basic Medium
Authors: Rabia Mohamed , Abdallah Alnuwaiser Maha , Hasan Fuead , Adel A. Abdelazeez Ahmed
Published: October 2023

In this study, O-amino benzenethiol (OABT) was oxidized in one pot using Fe(NO3)3 to produce a novel Fe2S3-Fe2O3/poly O-amino benzenethiol (POABT), which showed a highly uniform morphology. At the same time, from SEM analysis, highly wrinkled porous polypyrrole (Ppy) and porous ball-like POABT structures prepared from the K2S2O8 route were evidenced by SEM and TEM analyses. A nanocomposite pseudo-supercapacitor (SC) was fabricated using Fe2S3-Fe2O3/POABT, and its performance was tested with and without incorporating Ppy in the paste. The results indicate that Ppy significantly increased the specific capacitance (CS) values, indicating an enhancement in charge storage. At a current density of 0.2 A/g, the CS values were 44 F/g and 161 F/g for the paste without and with Ppy, respectively. Additionally, the E was calculated, and the incorporation of Ppy resulted in a significant increase in E, reaching 30 W.h.kg−1; this was significantly higher than the value of 8.18 W.h.kg−1 observed without Ppy materials. This effect is likely due to the improved charge transfer facilitated by the presence of Ppy, as evidenced by the Nyquist plot, where the Rct values were 1.1 Ω and 2.1 Ω with and without Ppy, respectively. Overall, the low cost and significant technical advantages of this capacitor make it a promising candidate for commercial applications.
Study on the Impact of a Combination of Synthetic Wollastonite and 2-Mercaptobenzothiazole-Based Fillers on UHMWPE Polymeric Matrix
Authors: Danilova Sakhayana N. , Okhlopkova Aitalina A. , Yarusova Sofia B. , Dyakonov Afanasy A. , Gordienko Pavel S. , Papynov Evgeniy K. , Shichalin Oleg O. , Buravlev Igor Yu. , Vasilev Andrey P. , Zhevtun Ivan G. , Ivanenko Natalya V.
Published: October 2023

This study investigates the impact of a binary filler on the physicomechanical and tribological properties, as well as structure, of polymeric composite materials based on ultra-high-molecular-weight polyethylene. The organic modifier—2-mercaptobenzothiazole and wollastonite particles synthesized from two different systems (modeled and derived from waste) were used as the binary filler. The synthesis of wollastonite was carried out in the complex model system (CaSO4·2H2O–SiO2·nH2O–KOH–H2O) and from technogenic waste (borogypsum). It was demonstrated that the introduction of the binary filler made it possible to obtain an optimal combination of mechanical and tribological properties. It was found that during the wear of polymeric composite materials loaded with organic fillers, the fillers migrate to the friction surface, providing a shield against abrasive wear of the steel counterface. Due to the modification of ultra-high-molecular-weight polyethylene by 2-mercaptobenzothiazole, the interdiffusion of polymeric matrix macromolecules and interphase coupling with wollastonite particles improve. The 2-mercaptobenzothiazole organic compound used as the filler facilitates the relaxation processes within the composite under external loads.
Preparation of a Novel Nanostructured Lead Titanate Composite Photocatalyst and Its Degradation of Organic Dyes under Visible Light
Authors: Nien Yu-Hsun , Lin Yan-Liang , Syu Zih-Jie , Liu Ming-Sheng , Huang Ting-Yu
Published: October 2023

Photocatalytic dye degradation is an energy-saving, environmentally friendly and sustainable way of managing potentially toxic wastewater from the textile industry. PbTiO3 was prepared using a solid-state reaction method, and an optimal ratio of PbTiO3/TiO2/g-C3N4 photocatalyst was synthesized using the sol-gel method to test its ability to decompose organic dyes. Methylene blue (MB) was selected as a target dye to test the photocatalytic effects. SEM results showed that the synthesis of a PbTiO3/TiO2/g-C3N4 photocatalyst yielded a unique nano structure with many surface pores. UV-Vis analysis demonstrated that the novel composite photocatalyst had higher visible light absorption, and a reduced energy gap. Experimental results showed that of the samples tested, PTO/TO/CN 1.0 showed the best photocatalytic effect on the removal of MB. Under visible light, the removal rate of MB by PTO/TO/CN 1.0 was up to 98.79%. The novel PTO/TO/CN 1.0 photocatalyst exhibited relatively high MB adsorption and had a high photocatalytic ability.
Injection Compression Molding of LDS-MID for Millimeter Wave Applications
Authors: Wolf Marius , Werum Kai , Eberhardt Wolfgang , Günther Thomas , Zimmermann André
Published: October 2023

LDS-MIDs (laser direct structured mechatronic integrated devices) are 3D (three-dimensional) circuit carriers that are used in many applications with a focus on antennas. However, thanks to the rising frequencies of HF (high-frequency) systems in 5G and radar applications up to the mmWave (millimeter wave) region, the requirements regarding the geometrical accuracy and minimal wall thicknesses for proper signal propagation in mmWave circuits became more strict. Additionally, interest in combining those with 3D microstructures like trenches or bumps for optimizing transmission lines and subsequent mounting processes is rising. The change from IM (injection molding) to ICM (injection compression molding) could offer a solution for improving the 3D geometries of LDS-MIDs. To enhance the scientific insight into this process variant, this paper reports on the manufacturing of LDS-MIDs for mmWave applications. Measurements of the warpage, homogeneity of local wall thicknesses, and replication accuracy of different trenches and bumps for mounting purposes are presented. Additionally, the effect of a change in the manufacturing process from IM to ICM regarding the dielectric properties of the used thermoplastics is reported as well as the influence of ICM on the properties of LDS metallization—in particular the metallization roughness and adhesion strength. This paper is then concluded by reporting on the HF performance of CPWs (coplanar waveguides) on LDS-MIDs in comparison to an HF-PCB.
Technological Advancements in Field Investigations of Marine Microorganisms: From Sampling Strategies to Molecular Analyses
Authors: Huang Zhishan , Fang Fang , Ding Lingyun , Yu Ke , Zhang Lijuan , Lu Hailong
Published: October 2023

The special characteristics of ocean ecosystems, such as the high salinity and pressure, low temperature, and nutrition, makes marine microorganisms diverse in species, gene composition, and ecological functions. Recent advances in molecular biology techniques, together with the ongoing developments in bioinformatic and automatic technologies, have highlighted the scientific studies on marine microbial ecology, eliminating the total reliance on classical cultivation-based techniques. This review systematically summarizes the innovative aspects of a field investigation of marine microorganisms. It covers various cutting-edge sampling approaches employed in the field, highlighting the in situ high-fidelity sampling strategies with practical applications. Particular emphasis is placed on the molecular analyses for marine ecology based on recently developed omics technologies. The key technical issues and substantial contribution of the contemporary instrumental analyses are discussed accordingly. It provides references for the in situ surveys of complex biogeochemical processes from marine microorganisms to ecosystems, relying on the development of new technical concepts and scientific methodologies in field investigations.