bluuebunny/crossref_metadata_2025 huggingface dataset:
Smoke Precipitation by Exposure to Dual-Frequency Ultrasonic Oscillations
Authors: Khmelev Vladimir , Shalunov Andrey , Tsyganok Sergey , Danilov Pavel
Published: December 2024

The analysis conducted herein has shown that the efficiency of smoke precipitation can be improved by additionally making smoke particles interact with ultrasonic (US) oscillations. Because the efficiency of US coagulation lowers when small particles assemble into agglomerates, the authors of this work have suggested studying how smoke particles interact with complex sound fields. The fields are formed by at least two US transducers which work at a similar frequency or on frequencies with small deviations. To form these fields, high-efficiency bending wave ultrasonic transducers have been developed and suggested. It has been shown that a complex ultrasonic field significantly enhances smoke precipitation. The field in question was constructed by simultaneously emitting 22 kHz US oscillations with a sound pressure level no lower than 140 dB at a distance of 1 m. The difference in US oscillations’ frequencies was no more than 300 Hz. Due to the effect of multi-frequency ultrasonic oscillations induced in the experimental smoke chamber, it was possible to provide a transmissivity value of 0.8 at a distance of 1 m from the transducers and 0.9 at a distance of 2 m. Thus, the uniform visibility improvement and complete suppression of incoming smoke was achieved. At the same time, the dual-frequency effect does not require an increase in ultrasonic energy for smoke due to the agglomeration of small particles under the influence of high-frequency ultrasonic vibrations and the further aggregation of the formed agglomerates by creating conditions for the additional rotational movement of the agglomerates due to low-frequency vibrations.
DNA Barcodes for Identifying Fish Egg Species Diversity in Summer and Autumn in the Southwest Daya Bay, China
Authors: Zheng Shile , Lin Jianbin , Wu Fengxia , Rao Yiyong , Wang Jinrun , He Siyuan , Huang Honghui , Hou Gang
Published: December 2024

Identifying fish eggs and understanding fish reproductive periods are necessary for informed fishery management. However, accurate the identification of fish eggs is difficult because eggs have few distinct characters, and their morphology varies ontogenetically. Using cytochrome c oxidase subunit I, we identified fish eggs from ichthyoplankton samples collected in the summer and autumn of 2021 from southwestern Daya Bay, China. Of 567 fish eggs, 498 high-quality cytochrome c oxidase subunit I sequences were obtained, of which 116 eggs (23.3%) could be identified to species; 364 (73.1%) to genus, family and/or order; and 18 (3.6%) could not be assigned. Of 51 apparent taxa, 46 were identified to 6 orders, 19 families, and 30 genera; 20 to the species and 25 to the genus and/or family, and 1 to the order. Among these 51 taxa, 35 occurred in summer, 29 occurred in autumn, and 13 occurred in both seasons; 22 occurred only in summer and 16 only in autumn, indicating species-specific spawning periods. High-resolution photographs of eggs are provided to facilitate subsequent identification based on morphology. These results will facilitate the identification of spawning grounds and their protection, to more holistically manage fishery resources in Daya Bay, China.
Effects of Mango Seed (Mangifera indica) Powder on Growth Performance, Immune Response, Gut Morphology, and Gene Expression of Nile Tilapia (Oreochromis niloticus)
Authors: Fontana Camilla Maria , Sumon Md Afsar Ahmed , Wannavijit Supreya , Lubis Anisa Rilla , Khongdee Nuttapon , Linh Nguyen Vu , Phimolsiripol Yuthana , Hoseinifar Seyed Hossein , Van Doan Hien
Published: December 2024

This study explored the effects of mango seed (MS) powder supplementation on the growth, immune response, gene expression, and intestinal morphology of Nile tilapia (Oreochromis niloticus) over an 8-week period. A total of 300 Nile tilapia fingerlings (average weight of 15.29 ± 0.05 g) were divided into five treatment groups and fed either a basal diet or one of four experimental diets containing MS powder at concentrations of 10 (MS10), 20 (MS20), 40 (MS40), and 80 (MS80) g kg−1. The results demonstrated that Nile tilapia fed MS-supplemented diets experienced significant improvements (p < 0.05) in weight gain (WG), specific growth rate (SGR), and survival rate (SR) compared to the control group (0 g kg−1 MS). The MS-treated groups also showed a significant increase (p < 0.05) in the height and branching of intestinal villi along the entire length of the intestine, as well as a significantly higher villus-to-crypt depth ratio (V/C), indicating enhanced intestinal health and functionality. Moreover, although MS supplementation did not increase peroxidase activity, it did lead to a significant increase (p < 0.05) in the activity of skin mucus and serum lysozyme, along with upregulated gene expression of immune-related (IL-1, IL-8, and LBP) and antioxidant genes (GST-α, GPX, and GSR). Polynomial regression analysis identified an optimal MS dosage of 36.43–45 g kg−1 for effectively improving growth, immunity, and immuno-oxidant gene expression in Nile tilapia. These results emphasize mango seed (MS) as a promising natural supplement for improving the diet of Nile tilapia and, potentially, other freshwater fish widely used in aquaculture.
Pathological Progress of Two Types of Nodules in Micropterus salmoides Infected with Nocardia seriolae
Authors: Li Chengwei , Wang Wenhui , Wu Fan , Pu Hao , Liao Lei
Published: December 2024

The typical clinical signs of Nocardia seriolae infection include white nodules, ranging from 0.1 to 10 mm in diameter, distributed across various internal organs. However, the structural composition of nodules of different sizes remains unexplored. In this study, natural cases of largemouth bass (Micropterus salmoides) were collected, and pathogenic bacteria were isolated and confirmed through a re-infection experiment. The isolated bacteria were identified as N. seriolae through 16S rRNA and gyrB gene sequencing. Healthy largemouth bass were infected with the isolate using an immersion infection and observed continuously over 56 days. Samples were successfully obtained from the incubation, prodromal, symptomatic, and convalescent phases, allowing for gross, histological, and ultrastructural observations of nodular lesion progression. Results demonstrated two types of nodular lesions: necrotic foci and granulomas. Macroscopically visible nodules larger than 1 mm, observed primarily in the liver, spleen, kidney, and muscle tissues of moribund fish, exhibited coagulative necrosis and were identified as the principal cause of mortality. Conversely, granulomas, with diameters less than 1 mm, were consistently present in the spleen, kidney, and liver during the symptomatic and convalescent phases.
Evaluation of Alternative Dietary Ingredients as a Sustainable and Ecological Solution for Meagre (Argyrosomus regius) Production in Earthen Ponds
Authors: Matias Ana Catarina , Quental-Ferreira Hugo , Dias Jorge , Saavedra Margarida , Bandarra Narcisa Maria , Araújo Ravi Luna , Gamboa Margarida , Soares Florbela , Pousão-Ferreira Pedro
Published: December 2024

The aquaculture sector is developing sustainability measures to address resource limitations and environmental concerns. A key strategy is replacing fishmeal and fish oil with alternatives that can equally sustain fish health, growth, and water quality. This study compared a standard diet (STD) to an alternative diet (ALT) containing sustainable ingredients, such as plant-based proteins and animal by-products, for meagre raised in earthen ponds within a polyculture system. Over 150 days, 5400 meagre juveniles (174.9 ± 32.8 g) were fed these diets. Fish on the ALT diet showed superior growth, likely due to higher dietary protein content and reduced protein degradation in liver and muscle, leading to increased protein content and reduced levels of dry matter, lipid, ash, energy, and phosphorous. While muscle cohesiveness was affected, fiber area and density were unchanged. ALT-fed fish exhibited higher saturated (SFA) and polyunsaturated (PUFA) fatty acids, reflecting the diet. Water quality indicators, including ammonia, nitrites, nitrates, and phosphates, were similar across diets, though chlorophyll a was higher in ponds with STD-fed fish. Overall, the ALT diet emerges as a sustainable alternative to the STD diet, maintaining or enhancing protein levels while reducing fishmeal usage. This approach effectively supports meagre growth and fillet quality without significant additional environmental impact.
Investigating the Effects of Labeled Data on Parameterized Physics-Informed Neural Networks for Surrogate Modeling: Design Optimization for Drag Reduction over a Forward-Facing Step
Authors: Gustafsson Erik , Andersson Magnus
Published: December 2024

Physics-informed neural networks (PINNs) are gaining traction as surrogate models for fluid dynamics problems, combining machine learning with physics-based constraints. This study investigates the impact of labeled data on the performance of parameterized physics-informed neural networks (PINNs) for surrogate modeling and design optimization. Different training approaches, including physics-only, data-only, and several combinations of both, are evaluated using fully connected (FCNN) and Fourier neural network (FNN) architectures. The test case focuses on reducing drag over a forward-facing step through optimal placement and sizing of an upstream obstacle. Results demonstrate that the inclusion of labeled data significantly enhances the accuracy and convergence rates of FCNNs, particularly in predicting flow separation and recirculation regions, and improves the stability of design optimization outcomes. Conversely, FNNs exhibit inconsistent responses to parameter changes when trained with labeled data, suggesting limitations in their applicability for certain design optimization tasks. The findings reveal that FCNNs trained with a balanced integration of data and physics constraints outperform both data-only and physics-only models, highlighting the importance of optimizing the training approach based on the specific requirements of fluid mechanics applications.
Combining CFD and AI/ML Modeling to Improve the Performance of Polypropylene Fluidized Bed Reactors
Authors: Ghasem Nayef
Published: December 2024

Polypropylene is one of the most widely used polymers in various applications, ranging from packaging materials to automotive components. This paper proposes the Computational Fluid Dynamics (CFD) and AI/ML simulation of a polypropylene fluidized bed reactor to reduce reactor loss and facilitate process understanding. COMSOL Multiphysics 6.2® solves a 2D multiphase CFD model for the reactor’s complex gas–solid interactions and fluid flows. The model is compared to experimental results and shows excellent predictions of gas distribution, fluid velocity, and temperature gradients. Critical operating parameters like feed temperature, catalyst feed rate, and propylene inlet concentration are all tested to determine their impact on the single-pass conversion of the reactor. The simulation simulates their effects on polypropylene yield and reactor efficiency. It also combines CFD with artificial intelligence and machine learning (AI/ML) algorithms, like artificial neural networks (ANN), resulting in a powerful predictive tool for accurately predicting reactor metrics based on operating conditions. The multifaceted CFD-AI/ML tool provides deep insight into improving reactor design, and it also helps save computing time and resources, giving industrial polypropylene plant growth a considerable lift.
Characterization of the Detailed Fatty Acid Profiles of a Large Number of Types of Cheese from the Mountains and Plains
Authors: Bittante Giovanni , Amalfitano Nicolò , Tagliapietra Franco , Schiavon Stefano , Cipolat-Gotet Claudio , Stocco Giorgia
Published: December 2024

The aim of this study was to characterize the highly detailed fatty acid (FA) profiles of 258 cheeses of 18 different categories of cheese collected in the mountains and on the plains of the Veneto region (Italy). The results clearly showed that, aside from the distinctive FA profiles of goat cheeses (more short-chain FAs and fewer MUFAs), the three categories of Formaggio di Malga (artisanal cheeses produced on temporary summer farms on Alpine pastures where transhumance is practiced) were very different from the other cheese categories in terms of their much higher CLA and omega-3 contents. Two categories of cheese from permanent farms in the mountains (Morlacco del Grappa and Monte Veronese PDO) were intermediate, and two other categories of cheeses originating in the mountains (Asiago PDO and Montasio PDO), but now produced mainly on the plains, were not distinguishable from the other cheese categories. The very detailed profile (65 individual FA, 11 isomers, and 12 groups of FAs) and the large number of cheese types analyzed (18) may represent a useful reference for future investigations, especially on the causes of variability in FAs and on their relationships with sensory properties and nutrition/health in humans.
Influences of Growth-Related Myopathies on Peptide Patterns of In Vitro Digested Cooked Chicken Breast and Stress-Related Responses in an Intestinal Caco-2 Cell Model
Authors: Malila Yuwares , Saensa-ard Sunitta , Kunyanee Chanikarn , Petpiroon Nalinrat , Kosit Nantanat , Charoenlappanit Sawanya , Phaonakrop Narumon , Srimarut Yanee , Aueviriyavit Sasitorn , Roytrakul Sittiruk
Published: December 2024

The objective of this study was to determine the effects of growth-related myopathies, i.e., normal, wooden breast (WB), white striping (WS), and the combined lesions of WS and WB (WS + WB), on the molecular response of Caco-2 cells. A total of 24 cooked chicken breasts (n = 6 per myopathy) was subjected to an in vitro digestion using an enzymatic process mimicking human gastrointestinal digestion. Based on peptidomics, in vitro protein digestion of the abnormal samples, particularly WB meat, resulted in more peptides with lower molecular mass relative to those of normal samples. The cooked meat hydrolysates obtained at the end of the digestion were applied to a Caco-2 cell model for 4 h. The cell viability of treated normal and abnormal samples was not different (p ≥ 0.05). Absolute transcript abundances of genes associated with primary oxidative stress response, including nuclear factor erythroid 2 like 2, superoxide dismutase, and hypoxia-inducible factor 1 were determined using a droplet digital polymerase chain reaction. No significant differences in transcript abundance of those genes in Caco-2 cells were demonstrated between normal and the abnormal samples (p ≥ 0.05). Overall, the findings supported that, compared to normal meat, the cooked chicken meat with growth-related myopathies might be digested and absorbed to a greater extent. The cooked abnormal meat did not exert significant transcriptional impacts regarding oxidative stress on the human epithelial Caco-2 cells.
Maize Kernel Broken Rate Prediction Using Machine Vision and Machine Learning Algorithms
Authors: Fan Chenlong , Wang Wenjing , Cui Tao , Liu Ying , Qiao Mengmeng
Published: December 2024

Rapid online detection of broken rate can effectively guide maize harvest with minimal damage to prevent kernel fungal damage. The broken rate prediction model based on machine vision and machine learning algorithms is proposed in this manuscript. A new dataset of high moisture content maize kernel phenotypic features was constructed by extracting seven features (geometric and shape features). Then, the regression model of the kernel (broken and unbroken) weight prediction and the classification model of kernel defect detection were established using the mainstream machine learning algorithm. In this way, the defect rapid identification and accurate weight prediction of broken kernels achieve the purpose of broken rate quantitative detection. The results prove that LGBM (light gradient boosting machine) and RF (random forest) algorithms were suitable for constructing weight prediction models of broken and unbroken kernels, respectively. The r values of the models built by the two algorithms were 0.985 and 0.910, respectively. SVM (support vector machine) algorithms perform well in constructing maize kernel classification models, with more than 95% classification accuracy. A strong linear relationship was observed between the predicted and actual broken rates. Therefore, this method could help to be an accurate, objective, efficient broken rate online detection method for maize harvest.
Debranched Lentil Starch–Sodium Alginate-Based Encapsulated Particles of Lacticaseibacillus rhamnosus GG: Morphology, Structural Characterization, In Vitro Release Behavior, and Storage Stability
Authors: Zhang Jinxiu , Hu Xinzhong , Ma Zhen
Published: December 2024

Starches with different degrees of debranching (DBS30, DBS60, and DBS90) and sodium alginate were used as the wall material for encapsulating particles of Lacticaseibacillus rhamnosus GG (LGG). The structural characteristics of these encapsulated particles were examined, along with the impact of varying levels of debranching on the encapsulation efficiency, the in vitro release of LGG under the simulated gastrointestinal environment, and the storage stability of the encapsulated particles. The results revealed a transformation in the crystalline polymorph from C- to B+V-type following debranching and retrogradation. This process also resulted in a significant decrease in molecular weight and polydispersity index, accompanied by an increase in amylose and resistant starch levels along with the relative crystallinity of the debranched lentil starch. Comparatively, DBS60-LGG and DBS90-LGG exhibited higher encapsulation efficiency and encapsulation yield than UDBS-LGG and DBS30-LGG. Furthermore, these encapsulated particles provided enhanced protection for LGG in both the simulated gastrointestinal environment and the storage process. It can be inferred that a superior encapsulation performance of the debranched lentil starch–sodium alginate-based encapsulated LGG particles was associated with higher debranching levels, a more uniform molecular weight distribution, and a more ordered multi-scale structure of the debranched lentil starch.
A Plant-Based Food Guide Adapted for Low-Fat Diets: The VegPlate Low-Fat (VP_LF)
Authors: Baroni Luciana , Rizzo Gianluca , Zavoli Martina , Battino Maurizio
Published: December 2024

Strong evidence supports the paramount importance of the composition of the diet for health. Not only diet should provide nutritional adequacy, but some foods and dietary components can also support the management of common chronic diseases, with mechanisms independent of nutritional adequacy. Among the various intervention diets, low-fat vegan diets have been shown to be effective for cardiometabolic health, mainly influencing insulin resistance, adiposity, and blood lipids. This type of diet relies on reducing or eliminating all added fats and choosing low-fat foods, mainly unprocessed whole-plant foods. We hereby propose a tool for planning low-fat vegan diets, the VegPlate Low-Fat (VP_LF), which has been obtained from a specific adaptation of the VegPlate method, which was already presented in previous publications for adults and some life stages and situations. The reduction in fats in the diet, which ranges between 10% and 15% of total energy, and the varied inclusion of foods from plant groups make it easier to provide adequate amounts of all nutrients with a normal- or lower-calorie intake, in comparison with diets that do not limit fat intakes. We expect that this new proposal will help nutrition professionals embrace low-fat diets as a first-line intervention for individuals affected by different health conditions who can benefit from these diets.
Effect of High-Pressure Processing Pretreatment on the Textural Properties of Cooked Nuovo Maratelli Rice
Authors: Arroqui Cristina , Horvitz Sandra , Noriega María José , Fernández-Pan Idoya , Ibañez Francisco C. , Vírseda Paloma
Published: December 2024

Nuovo Maratelli, a japonica rice with an intermediate amylose content, is suitable for paella (a traditional Spanish dish) due to its ability to withstand cooking and absorb flavors. In this study, high-pressure processing (HPP) at 400 and 600 MPa (10 min) was used as a pretreatment to improve the properties of rice cooked by either boiling or microwaving. The microstructure and pasting properties of unpressurized and pressurized rice were examined. Also, the cooking time and cooking kinetics were determined for each cooking method. Overall, the pasting properties of the rice were not impacted by the HPP treatments, but the typical polyhedral form of the rice starch granules was lost, especially at 600 MPa. Cooking times were reduced from 14 and 10 min for unpressurized samples to 12 and 8 min (400 MPa) and 8 and 6 min (600 MPa) for boiling- and microwave-cooked rice, respectively. The rice pretreated at 400 MPa (10 min) and microwaved (8 min) had lower hardness and adhesiveness, which was linked to the release of amylose during cooking. In summary, HPP could be an effective pretreatment for the improvement of the cooking and textural properties of Nuovo Maratelli rice, particularly when cooked by microwaving.
Pattern Formation Mechanisms of Spatiotemporally Discrete Activator–Inhibitor Model with Self- and Cross-Diffusions
Authors: Li You , Sun Ying , Luo Jingyu , Pang Jiayi , Liu Bingjie
Published: December 2024

In this paper, we aim to solve the issue of pattern formation mechanisms in a spatiotemporally discrete activator–inhibitor model that incorporates self- and cross-diffusions. We seek to identify the conditions that lead to the emergence of complex patterns and to elucidate the principles governing the dynamic behaviors that result in these patterns. We first construct a corresponding coupled map lattice (CML) model based on the continuous activator–inhibitor reaction–diffusion system. In the reaction stage, we examine the existence, uniqueness, and stability of the homogeneous stationary state and specify the parametric conditions for realizing these properties. Furthermore, by applying the center manifold theorem, we perform a flip bifurcation analysis and confirm that the model is capable of undergoing flip bifurcation. In the diffusion stage, we focus on the analysis of Turing bifurcation and determine the parameter conditions for the emergence of Turing instability. Through numerical simulations, we validate and explain the results of our theoretical analysis. Our study reveals various Turing instability mechanisms by coupling Turing and flip bifurcations, which include pure-self-diffusion-Turing instability, pure-cross-diffusion-Turing instability, flip-self-diffusion-Turing instability, flip-cross-diffusion-Turing instability, and chaos-self-diffusion-Turing instability mechanisms. Under different mechanisms, we illustrate the corresponding Turing patterns and discover a rich variety of pattern types such as labyrinthine, mosaic, alternating mosaic, colorful mottled grid patterns with winding and twisted bands, and patterns with dense patches and twisted bands nested together. Our research provides a theoretical framework and numerical support for understanding the complex dynamical behaviors and pattern formations in activator–inhibitor models with self- and cross-diffusions.
Association of Systemic Inflammation with Dietary Intake, Nutrition Impact Symptoms, and Eating-Related Distress Among Patients with Advanced Cancer
Authors: Amano Koji , Koshimoto Saori , Okamura Satomi , Sakaguchi Tatsuma , Arakawa Sayaka , Matsuda Yoshinobu , Tokoro Akihiro , Takeuchi Takashi , Satomi Eriko , Wada Tamiki , Wada Makoto , Yamada Tomomi , Mori Naoharu
Published: December 2024

Background: Serum C-reactive protein (CRP) levels are correlated with patient outcomes in cancer. This study aimed to determine associations between the CRP level and the dietary intake, symptoms, and eating-related distress (ERD). Methods: We conducted a multicenter survey among advanced cancer patients. Information on patient characteristics was retrieved from the electronic medical records. Data on patient outcomes were obtained through the questionnaire. Patients were categorized into the low CRP group (<5 mg/dL) and the high CRP group (≥5 mg/dL). Comparisons were calculated using the Mann–Whitney U test or chi-squared test. To assess associations between CRP levels and ERD, multivariate logistic regression analysis was performed. Results: A total of 191 patients were enrolled and divided into the low CRP group (n = 117) and the high CRP group (n = 74). The high CRP group had a more reduced dietary intake (p = 0.002) and more severe appetite loss (p = 0.008). The total scores of the ERD questionnaire (both the long and short versions) were significantly higher in the high CRP group (p = 0.040 and 0.029). The high CRP group also had significantly higher risks for ERD, as assessed using the long and short versions of the questionnaire (odds ratio [OR] 2.13, 95% confidence interval [CI] 1.10–4.11; OR 2.06, 95% CI 1.05–4.05). Conclusions: High CRP levels were significantly associated with reduced dietary intake, appetite loss, and ERD. A serum CRP value of 5 mg/dL may be a useful indicator for initiating cancer cachexia care.
Validity and Reliability of the Basic Psychological Need Satisfaction and Frustration Scale Among Cancer Survivors in Korean Healthcare Contexts
Authors: Yeom Hyun-E , Lee Jungmin
Published: December 2024

Background/Objectives: Basic psychological needs are essential for fostering motivation, self-regulated behaviors, and overall well-being. For cancer survivors, fulfilling these needs is crucial for coping with the various challenges of survivorship and for enhancing psychosocial health. This study aimed to assess the validity and reliability of the Korean version of the Basic Psychological Needs Satisfaction and Frustration Scale (K-BPNSFS) in a cancer survivor population. Methods: A cross-sectional design was employed, involving 367 community-dwelling cancer survivors. Construct validity was assessed using confirmatory factor analysis with multiple fit indices, while convergent validity was examined through Pearson’s correlation coefficients. Reliability was evaluated using internal consistency, inter-item correlations, and item-total correlations. Results: The findings confirmed a robust six-factor structure of the K-BPNSFS, which includes satisfaction and frustration dimensions corresponding to autonomy, relatedness, and competence needs. Convergent validity was supported by significant correlations with relevant constructs, aligning with the scale’s theoretical underpinnings. Reliability analysis demonstrated high internal consistency across all dimensions, with strong Cronbach’s alpha values and substantial item-total and inter-item correlations. Conclusions: This study establishes the K-BPNSFS as a valid, reliable, and culturally relevant instrument for assessing the basic psychological needs of Korean cancer survivors. Application of this scale provides critical insights into the unique psychological needs of this population, supporting the development of targeted healthcare strategies to enhance intrinsic motivation, self-care, and overall quality of life.
The Sucrose Regulation of Plant Shoot Branching
Authors: Xiong Shifa , Wang Yangdong , Chen Yicun , Shi Xiang , Wu Liwen
Published: December 2024

The branching characteristics of plants represent crucial agronomic traits that significantly influence both yield and economic value. The formation of branches involves several stages, including the initiation of axillary meristems and the activation and continued growth of lateral buds. These processes are collaboratively regulated by genetic factors, hormones, nutritional availability, and environmental conditions. Recently, sucrose has emerged as a significant factor impacting plant branching characteristics. Sucrose not only serves as a carbon source, providing essential nutrition and energy for branching growth, but also integrates multiple regulatory factors to jointly influence branching development. This review summarizes the structural enzyme genes involved in the sucrose synthesis pathway and the key co-factors in signal transduction pathways, the interactions between sucrose and plant hormones and transcription factors, and the regulatory role of sucrose metabolites in plant branching. Furthermore, it highlights critical issues that require further investigation regarding the role of sucrose in regulating branching.
Effects of Irrigation Methods on Growth and Water Productivity in Bell Pepper Cultivation in Northern South Korea
Authors: Yang Hwichan , Bae Yunhyeong , Kim Youngho , Hyeon Soonjae , Choi Minseong , Yang Seongho , Kim Daehyun , Jang Dongcheol
Published: December 2024

Although the bell pepper (Capsicum annuum L.) is sensitive to water stress, little information is available on proper irrigation management methods for bell pepper cultivation in the northern South Korean climate. We compared the effects of different irrigation methods on crop growth and water productivity in two bell pepper varieties (Maldonado and Nagano) at different irrigation durations (ending 3 h before sunset and ending 4 h before sunset) and irrigation quantities (placing two, three, and four drippers capable of irrigating at 2 L·h−1) over approximately 280 days by performing in-depth analysis of various growth indicators. The plant height of Maldonado increased as the irrigation amount increased in all irrigation treatments of T1 and T2. In Nagano, there was no significant difference in plant height between D3 and D4. Overall, the irrigation treatments produced a higher difference in yield in Maldonado plants than in Nagano plants. WP tended to increase inversely to reduction in irrigation quantity in Groups 1 (May–June) and 4 (November–December) of both varieties, and response to irrigation stop time varied among the varieties. We inferred that the optimal irrigation method for bell pepper cultivation in northern South Korea is to supply irrigation at the D3 level and adjust the irrigation end time according to the variety and crop strength.
Gut Microbiota and Liver Dysfunction in Sepsis: The Role of Inflammatory Mediators and Therapeutic Approaches
Authors: Shahid Aqsa , Chambers Stephen , Scott-Thomas Amy , Bhatia Madhav
Published: December 2024

Sepsis is a life-threatening complication caused by an uncontrolled immune response to infection that can lead to multi-organ dysfunction, including liver injury. Recent research has shown the critical role of gut microbiota in sepsis pathogenesis, with the gut–liver axis playing a crucial role in disease progression. Mechanisms such as the disruption of the gut barrier and liver injury pathways mediated by cytokines, chemokines, adhesion molecules, hydrogen sulfide (H2S). and substance P (SP) have been the focus of recent studies. Some potential biomarkers and gut microbiota-targeted therapies have shown promise as emerging tools for predicting and managing sepsis. This review describes the role of the gut–liver axis in sepsis and the potential of microbiota-targeted therapies and biomarker-driven interventions to improve sepsis outcomes.
Epigenetic and Cellular Reprogramming of Doxorubicin-Resistant MCF-7 Cells Treated with Curcumin
Authors: Poma Paola , Rigogliuso Salvatrice , Labbozzetta Manuela , Nicosia Aldo , Costa Salvatore , Ragusa Maria Antonietta , Notarbartolo Monica
Published: December 2024

The MCF-7R breast cancer cell line, developed by treating the parental MCF-7 cells with increasing doses of doxorubicin, serves as a model for studying acquired multidrug resistance (MDR). MDR is a major challenge in cancer therapy, often driven by overexpression of the efflux pump P-glycoprotein (P-gp) and epigenetic modifications. While many P-gp inhibitors show promise in vitro, their nonspecific effects on the efflux pump limit in vivo application. Curcumin, a natural compound with pleiotropic action, is a nontoxic P-gp inhibitor capable of modulating multiple pathways. To explore curcumin’s molecular effects on MCF-7R cells, we analyzed the expression of genes involved in DNA methylation and transcription regulation, including ABCB1/MDR1. Reduced representation bisulfite sequencing further unveiled key epigenetic changes induced by curcumin. Our findings indicate that curcumin treatment not only modulates critical cellular processes, such as ribosome biogenesis and cytoskeletal dynamics, but also reverses the resistant phenotype, toward that of sensitive cells. This study highlights curcumin’s potential as an adjuvant therapy to overcome chemoresistance, offering new avenues for pharmacological strategies targeting epigenetic regulation to re-sensitize resistant cancer cells.
Special Issue: “Enzymes and Enzyme Inhibitors—Applications in Medicine and Diagnosis 2.0”
Authors: Geronikaki Athina
Published: December 2024

The first paper in this Special Issue explores the synthesis, characterization, biological, and catalytic activities of new gold(I) and silver(I) complexes that are stabilized by caffeine derivatives and used as NHC ligands [...]
Systematic Review of Sarcopenia Biomarkers in Hip Fracture Patients as a Potential Tool in Clinical Evaluation
Authors: Brzeszczyński Filip , Hamilton David , Bończak Oktawiusz , Brzeszczyńska Joanna
Published: December 2024

Hip fractures are associated with high morbidity and mortality. Sarcopenia is a significant factor contributing to poor prognosis; however, the clinical diagnosis of sarcopenia remains difficult in surgical patients. This systematic review aims to identify the biomarkers of sarcopenia as diagnostic and predictive tools in patients admitted for hip fracture surgery. A systematic search was conducted in the MEDLINE, EMBASE, and Google Scholar databases according to the PRISMA guidelines. Biomarker study quality was assessed using the BIOCROSS score. A total of 7 studies met the inclusion criteria and 515 patients were included, of whom 402 (78%) were female and 113 (22%) were male. The mean age of the participants was 83.1 years (SD: 5.9). Skeletal muscle biopsies were used for biomarker assessment in 14% (1/7) of studies and venous blood samples were used in the remaining 86% (6/7). The highlighted sarcopenia biomarkers included the low expression of insulin-like growth factor (IGF-I) and tumor necrosis factor-α (TNF-α), along with high serum myostatin and low serum vitamin D levels. Overall, the BIOCROSS score was satisfactory, with all studies obtaining at least a score of 13/20. The orthopedic literature is limited; however, the highlighted biomarkers in this review could be used as adjuncts in the diagnosis of sarcopenia in surgical patients.
Preliminary Study on the Positive Expression Regulation of Alpha2-Macroglobulin in the Testicular Tissue of Male Mice by Environmental Estrogens
Authors: Li Hong-Mei , Gao Yan-Rong , Liu Chang , Sheng Yu-Xin , Pu Ya-Jia , Sun Jia-He , Tian Ya-Nan , Yang Li , Ma Hui-Ming , Xu Hai-Ming
Published: December 2024

The male reproductive impairment caused by environmental estrogens (EEs) stands as a pivotal research area in environmental toxicology. Alpha2-macroglobulin (A2M) emerges as a promising molecule capable of counteracting oxidative stress induced by EEs. This study conducted exposure experiments spanning PND1 to PND56 employing ICR mice, aiming to delve into the expression patterns of A2M and its modulated IL-6 in the testicular tissue of mice subsequent to diethylstilbestrol (DES) and benzophenone (BP) exposure, while elucidating the pivotal role of ERs in this intricate process. Our findings revealed that upon DES exposure (10 and 100 nM), there was a pronounced upregulation of A2M (mRNA and in situ protein levels) in mouse testicular tissue. Similarly, exposure to BPs (BP-1, BP-2, and BP-3, each at 10 and 1000 nM) exhibited comparable effects and increasing A2M levels in serum. Notably, BP exposure also caused an elevation in IL-6 levels (which could be directly regulated by A2M) within testicular tissue (mRNA and in situ protein). Remarkably, the specific estrogen receptor antagonist ICI 182780 (0.5 mg/kg/day) was effective in reversing the upregulation of both A2M and IL-6 induced by BP exposure. Significantly, the results of theoretical prediction of the potential ERE site in the A2m gene promoter region and ChIP-qPCR experiment provide essential and strong evidence for the key conclusion that A2m is the target gene of ER. Taken together, our study highlights EEs’ ability to regulate A2M expression in the male reproductive system via the ER signaling pathway. This vital insight deepens our understanding of molecular mechanisms protecting against oxidative stress caused by EEs.
Inflammatory Effects and Regulatory Mechanisms of Chitinase-3-like-1 in Multiple Human Body Systems: A Comprehensive Review
Authors: Liu Dong , Hu Xin , Ding Xiao , Li Ming , Ding Lei
Published: December 2024

Chitinase-3-like-1 (Chi3l1), also known as YKL-40 or BRP-39, is a highly conserved mammalian chitinase with a chitin-binding ability but no chitinase enzymatic activity. Chi3l1 is secreted by various cell types and induced by several inflammatory cytokines. It can mediate a series of cell biological processes, such as proliferation, apoptosis, migration, differentiation, and polarization. Accumulating evidence has verified that Chi3l1 is involved in diverse inflammatory conditions; however, a systematic and comprehensive understanding of the roles and mechanisms of Chi3l1 in almost all human body system-related inflammatory diseases is still lacking. The human body consists of ten organ systems, which are combinations of multiple organs that perform one or more physiological functions. Abnormalities in these human systems can trigger a series of inflammatory environments, posing serious threats to the quality of life and lifespan of humans. Therefore, exploring novel and reliable biomarkers for these diseases is highly important, with Chi3l1 being one such parameter because of its physiological and pathophysiological roles in the development of multiple inflammatory diseases. Reportedly, Chi3l1 plays an important role in diagnosing and determining disease activity/severity/prognosis related to multiple human body system inflammation disorders. Additionally, many studies have revealed the influencing factors and regulatory mechanisms (e.g., the ERK and MAPK pathways) of Chi3l1 in these inflammatory conditions, identifying potential novel therapeutic targets for these diseases. In this review, we comprehensively summarize the potential roles and underlying mechanisms of Chi3l1 in inflammatory disorders of the respiratory, digestive, circulatory, nervous, urinary, endocrine, skeletal, muscular, and reproductive systems, which provides a more systematic understanding of Chi3l1 in multiple human body system-related inflammatory diseases. Moreover, this article summarizes potential therapeutic strategies for inflammatory diseases in these systems on the basis of the revealed roles and mechanisms mediated by Chi3l1.
The Induction of Disease Resistance by Scopolamine and the Application of Datura Extract Against Potato (Solanum tuberosum L.) Late Blight
Authors: Zhu Zhiming , Liu Shicheng , Liu Yi , Zhang Xinze , Shi Zhiwen , Liu Shuting , Zhu Zhenglin , Dong Pan
Published: December 2024

Late blight, caused by Phytophthora infestans, is a devastating disease of potato. Our previous work illustrated that scopolamine, the main bioactive substance of Datura extract, exerts direct inhibitory effects on P. infestans, but it is unclear whether scopolamine and Datura extract can boost resistance to late blight in potato. In this study, P. infestans is used to infect scopolamine-treated potato pieces and leaves, as well as whole potatoes. We found that scopolamine-treated potato is resistant to P. infestans both in vitro and in vivo. The treatment of 4.5 g/L scopolamine reduces the lesion size of whole potato to 54% compared with the control after 20 d of the infection of P. infestans. The disease-resistant substance detection based on the kit method shows that scopolamine triggers the upregulation of polyphenoloxidase, peroxidase, superoxide dismutase activities, and H2O2 contents in potato tubers, and the decline of phenylalanine ammonia lyase and catalase activity. A total of 1682 significantly differentially expressed genes were detected with or without scopolamine treatment through high-throughput transcriptome sequencing and the DESeq2 software (version 1.24.0), including 705 upregulated and 977 downregulated genes. Scopolamine may affect the genes functioning in the cell wall, membrane and the plant-pathogen interaction. The addition of Datura extract could directly inhibit the mycelial growth of P. infestans on rye plate medium. In addition, P. infestans was found to be resistant to late blight in potato pieces treated with Datura extract. Datura extract can also be utilized in combination with the chemical fungicide Infinito in field experiments to lessen late blight symptoms and enhance potato yield. To our knowledge, this is the first study to detect the induction of disease resistance by scopolamine, and it also explores the feasibility of Datura extract in potato disease resistance.
Manganese(II) Complexes with Non-Steroidal Anti-Inflammatory Drugs: Structure and Biological Activity
Authors: Dimiza Filitsa , Hatzidimitriou Antonios G. , Psomas George
Published: December 2024

Nine manganese(II) complexes with a series of non-steroidal anti-inflammatory drugs (namely sodium diclofenac, diflunisal, flufenamic acid, sodium meclofenamate, mefenamic acid, and tolfenamic acid) were prepared in the presence of diverse nitrogen donors, i.e., pyridine, 1,10–phenanthroline, 2,2′–bipyridine and neocuproine, as co-ligands and were characterized with spectroscopic techniques and single-crystal X-ray crystallography. The biological profile of the resultant complexes was investigated regarding their antioxidant potency and their interaction with DNA and serum albumins. The complexes interact with calf–thymus DNA in an intercalative mode and bind tightly and reversibly to human and bovine serum albumins studied. In order to assess the antioxidant activity of the Mn(II) complexes, their ability to scavenge 2,2′–azinobis(3–ethylbenzothiazoline–6–sulfonic acid) free radicals was monitored.
Biomimetic Natural Biomaterial Nanocomposite Scaffolds: A Rising Prospect for Bone Replacement
Authors: Zaczek-Moczydłowska Maja A. , Joszko Kamil , Kavoosi Mahboubeh , Markowska Aleksandra , Likus Wirginia , Ghavami Saeid , Łos Marek J.
Published: December 2024

Biomimetic natural biomaterial (BNBM) nanocomposite scaffolds for bone replacement can reduce the rate of implant failure and the associated risks of post-surgical complications for patients. Traditional bone implants, like allografts, and autografts, have limitations, such as donor site morbidity and potential patient inflammation. Over two million bone transplant procedures are performed yearly, and success varies depending on the material used. This emphasizes the importance of developing new biomaterials for bone replacement. Innovative BNBM nanocomposites for modern bone fabrication can promote the colonization of the desired cellular components and provide the necessary mechanical properties. Recent studies have highlighted the advantages of BNBM nanocomposites for bone replacement; therefore, this review focuses on the application of cellulose, chitosan, alginates, collagen, hyaluronic acid, and synthetic polymers enhanced with nanoparticles for the fabrication of nanocomposite scaffolds used in bone regeneration and replacement. This work outlines the most up-to-date overview and perspectives of selected promising BNBM nanocomposites for bone replacement that could be used for scaffold fabrication and replace other biomorphic materials such as metallics, ceramics, and synthetic polymers in the future. In summary, the concluding remarks highlight the advantages and disadvantages of BNBM nanocomposites, prospects, and future directions for bone tissue regeneration and replacement.
Identification of Small RNAs in Streptomyces clavuligerus Using High-Resolution Transcriptomics and Expression Profiling During Clavulanic Acid Production
Authors: Caicedo-Montoya Carlos , Patiño Luisa F. , Ríos-Estepa Rigoberto
Published: December 2024

Small non-coding RNAs play a pivotal role in regulating various metabolic processes in both prokaryotic and eukaryotic organisms. However, knowledge about small RNAs (sRNAs) in Streptomyces clavuligerus (S. clavuligerus) is scarce. This study aimed to use cutting-edge bioinformatics tools and a compendium of RNA-seq data to predict the potential coding of sRNAs that might be present in the genome of S. clavuligerus ATCC 27064. In the genome of S. clavuligerus, 606 intergenic regions (IGRs) are conserved, and 272 possess a highly thermodynamically stable and conserved secondary structure, indicating the presence of non-coding RNA in these regions. The transcriptome assembly of S. clavuligerus showed that the genome is completely functional, as all the annotated genes are expressed under the conditions analyzed. From this assembly, transcripts originating from IGRs were labeled as putative sRNAs, and their differential expression during the growth curve of S. clavuligerus for clavulanic acid (CA) production was established. The interactome of these differentially expressed (DE) RNAs displayed the sRNAs as global regulators, as they can have multiple mRNA targets. The functional annotation of the target genes of DE sRNAs demonstrated that they are directly involved in secondary metabolite production. Specifically, two sRNA have the genes of the biosynthetic gene cluster of CA as targets. Thus, these molecules add an additional layer to the regulatory cascade for CA biosynthesis, and we propose them as targets for metabolic engineering to increase CA production.
The Potency of Cytotoxic Mechanisms of Local Anesthetics in Human Chondrocyte Cells
Authors: Chen Jia-Lin , Liu Shu-Ting , Wu Chia-Chun , Chen Yi-Chou , Huang Shih-Ming
Published: December 2024

Local anesthetics are commonly used in various clinical settings for both prevention and symptom relief. Numerous clinical studies have demonstrated that intra-articular injections of local anesthetics achieve high success rates in orthopedic practices. However, several widely used local anesthetics, including bupivacaine, lidocaine, and ropivacaine, have been shown to exhibit toxicity to chondrocytes, with the underlying mechanisms of chondrotoxicity remaining poorly understood. In this study, we aimed to investigate the cytotoxic effects of local anesthetics, specifically focusing on the consequences of a single intra-articular injection in human chondrocyte cells. Our results reveal that lidocaine, levobupivacaine, bupivacaine, and ropivacaine induced cell death, characterized by the induction of apoptosis and the suppression of cellular proliferation. These effects were mediated through mechanisms involving oxidative stress, mitochondrial dysfunction, and autophagy pathways. We found that the toxic effects of local anesthetics were concentration-dependent, with lidocaine exhibiting the lowest cytotoxicity among the tested agents in TC28a cells. Notably, bupivacaine and levobupivacaine displayed significant cytotoxic effects related to apoptosis, cellular proliferation, reactive oxygen species generation, mitochondrial membrane potential depolarization, and autophagy in human chondrocyte cells. Our findings not only support existing clinical studies but also highlight potential targets for developing protective agents to mitigate serious side effects associated with their use in orthopedic practices.
Correction: Yan et al. Embryonic Lethal Phenotyping to Identify Candidate Genes Related with Birth Defects. Int. J. Mol. Sci. 2024, 25, 8788
Authors: Yan Bing , Gong Baoming , Wang Xue , Zheng Yufang , Sun Lei , Wu Xiaohui
Published: December 2024

In the original publication [...]