bluuebunny/crossref_metadata_2025 huggingface dataset:
IL LINGUAGGIO PUBBLICITARIO AI TEMPI DEL COVID
Authors: Radenković Šošić Bojana
Published: November 2024

È stato confermato in numerose ricerche che la pubblicità non esprime solo significati di tipo commerciale, ma rappresenta uno specchio sociale e linguistico della realtà circostante. Gli inevitabili cambiamenti occorsi nella quotidianità e nello stile di vita come conseguenza della pandemia all’inizio del 2020 hanno portato a mutamenti visibili anche nella comunicazione pubblicitaria. Mettendo in secondo piano il focus sui prodotti pubblicizzati e nascondendo apparentemente i motivi persuasivi e commerciali, gli spot pubblicitari della prima fase del lockdown abbondavano di messaggi i quali riflettevano innanzitutto le funzioni emotive del linguaggio pubblicitario. Seguendo i fili narrativi principali della comunicazione pubblicitaria dall’inizio della pandemia fino alle fasi di ripresa (l’Italia e l’italianità, la contrapposizione fuori: dentro, il ruolo della tecnologia, il recupero dei momenti persi, il ruolo della vaccinazione) la lingua pubblicitaria si è adattata alle esigenze della nuova normalità. Le funzioni conative sono state sostituite da quelle poetiche e emotive. Dopo un certo periodo, il linguaggio pubblicitario è tornato nel focus delle ricerche accademiche e professionali. Lo scopo di questo articolo è quello di esaminare aspetti linguistici del linguaggio pubblicitario ai tempi del Covid tenendo in considerazione le peculiarità morfosintattiche, semantiche e retoriche della lingua pubblicitaria, nonché il rapporto fra il codice verbale e iconico usato nelle pubblicità di questo periodo.
Un parc de forêt dense en Afrique : le parc national de Taï (Côte d'Ivoire) : suite et fin
Authors: Bousquet B.
Published: November 2024

La première partie de cet article a été publiée dans le numéro précédent. Elle rappelait les objectifs visés, décrivait l'environnement, et énumérait les ressources.Dans cette deuxième partie, l'auteur traite de la configuration du parc et de sa zone de protection. Il indique également les meilleurs moyens possibles non seulement de sauvegarder le parc, mais aussi et surtout de le valoriser. Il estime que l'ouverture aux touristes serait bénéfique ; le parc serait mieux connu, mieux accepté par les populations locales, mieux protégé. Le Mont Nienokoué pourrait constituer un pôle d'attraction.
Mechanism of ferroptosis resistance in cancer cells
Authors: Wang Yuan , Yu Guifang , Chen Xin
Published: November 2024

Ferroptosis is an iron-dependent cell death characterized by increased intracellular lipid peroxidation. Inducing ferroptosis has shown significant potential in eliminating various malignancies. However, the effectiveness of ferroptosis-based treatments is hampered by the intrinsic or acquired resistance of some tumors. In this review, we delineate the known mechanisms that regulate ferroptosis sensitivity and summarize the therapeutic application of ferroptosis inducers in cancer. Additionally, we discuss the roles of diverse signaling pathways that contribute to ferroptosis resistance in cancer cells, including the glutathione (GSH) and coenzyme Q (CoQ) pathways, NFE2-like bZIP transcription factor 2 (NRF2) antioxidant response, and lipid and iron metabolism. This emerging knowledge may serve as a foundation for developing novel anticancer strategies to overcome ferroptosis resistance.
PERBANDINGAN BIAYA DAN WAKTU ANTARA METODE KONVENSIONAL DENGAN METODE MODULAR MENGGUNAKAN MICROSOFT PROJECT
Authors: Huwaida Nabila Yusriyya , Handayani Fajar Sri , Rifai Muji
Published: November 2024

Indonesia's construction sector is experiencing rapid growth, this proven by numerous development projects, including a sub-district building construction project, aiming to optimize community services.  A project must have three aspects, namely cost, quality and time. Choosing an implementation method is very important so that a project can achieve these three goals. The research method used a quantitative descriptive approach with the aim of comparing cost and time aspects between conventional methods and modular methods. The conventional approach involves working on site using conventional building materials, while the modular approach involves the use of pre-fabricated modules. In analyzing the costs and time of construction of the Grabag Sub-district office building, Purworejo Regency, Microsoft project application was used to calculate the duration of the work. The cost analysis show that building structure work using the conventional method costs Rp.3,086,929,413.11, while the modular method costs Rp.3,646,018,620.52. So the difference in the cost of construction work is Rp.559,089,207.42 or 18.11% higher for work using the modular method. The time analysis obtained building structure work using conventional methods require 150 days while the modular method is 87 days or time efficiency of 63 days or 42% lower for work with modular methods.  The results of the Time Cost Trade Off (TCTO) calculation showed that the total project cost for conventional construction was Rp.2,781,017,489 while the cost for modular construction was Rp.3,117,530,328. So a difference of Rp.336,512,839 is higher for modular construction. Therefore, conventional construction is the most economical option for the sub-district building.
Rancangan kemasan pelindung apel selama tranportasi berbahan dasar eceng gondok dan sabut kelapa
Authors: Widhiantari Ida Ayu , Amuddin Amuddin , Ridho Rosyid , Khalil Fakhrul Irfan , Puspitasari Isnaini
Published: November 2024

Packaging during the transportation process greatly influences the damage to horticultural products. Harvest apples are usually packed in wooden boxes or bamboo baskets and stacked until they reach maximum capacity. This makes the fruit easily damaged by bruising due to high piles. Based on these conditions, it is necessary to handle horticultural products in the form of apples to reduce damage to the fruit during transportation to maintain the quality of the apples until they reach consumers and extend the shelf life of the fruit. This research aims to design protective packaging for apples from a combination of water hyacinth and coconut fiber. The experimental method was carried out in this research with 2 factors consisting of 3 levels; factor one the combination of packaging materials, and factor two was the pressure of the packaging printer. Nine experimental units were obtained from the combination of the two treatments, namely K1T1 (E 90% + S 10%) with a pressing pressure of 400 psi, K1T1 (E 90% + S 10%) with a pressing pressure of 700 psi, K1T1 (E 90% + S 10% ) with a pressing pressure of 1000 psi, K2T1 (E 60% + S 40%) with a pressing pressure of 400 psi, K2T2 (E 60% + S 40%) with a pressing pressure of 700 psi, K2T3 (E 60% + S 40%) with pressing pressure 1000 psi, K3T1 (E 30% + S 70%) with pressing pressure 400 psi, K3T2 (E 30% + S 70%) with pressing pressure 700 psi, K3T3 (E 30% + S 70%) with pressing pressure 1000 psi. The results showed that the combination treatment of K2T2 with a pressing pressure of 700 psi provided the best protective quality for apples with the lowest percentage of mechanical damage and reduction in apple mass,  at 5.556%, 2.55%, respectively. The springiness and texture values for apples were the highest with values respectively 3.17 mm, 16.7335N. Meanwhile, the highest packaging compressive strength value was obtained in the K3T3 treatment with a value of 26.164N.
Conocimientos, actitudes y practicas sobre lactancia materna en puérperas del Hospital Nacional Docente Madre Niño San Bartolomé en el contexto de la pandemia de la COVID-19 en el 2022
Authors:   , Sanchez Rojas Danae Guadalupe , Tafur Milla Gabriela Emilia
Published: November 2024

Introducción: La leche humana es la fuente de nutrición primaria para los recién nacidos, proporcionando utilidad para la progenitora, así como para su recién nacido. Se sabe que el conocimiento adecuado además de buena actitud de las madres que dan de lactar, son elementos claves para una exitosa práctica de lactancia. No obstante, durante la pandemia debido al COVID-19 se plantearon desafíos significativos para la lactancia materna, como temor y preocupación generalizada al virus. Objetivo: Determinar el nivel de los conocimientos, las actitudes y prácticas de la lactancia materna en madres puérperas en el Hospital Nacional Docente Madre Niño San Bartolomé durante la pandemia por COVID- 19. Materiales y Métodos: La composición de la muestra incluyo a 346 mujeres postparto del Hospital Nacional Docente Madre Niño San Bartolomé. Donde se tomó una encuesta, validada previamente, en la que se recopiló información en relación con nuestro objetivo general acerca de la lactancia.Resultados: Los resultados revelaron que un 86.7% exhibieron niveles de alto conocimiento, la actitud fue positiva en un 91.3% y el 90.5% reportó prácticas adecuadas respecto a la lactancia. Además, se obtuvo que más del 50% de las madres encuestadas afirmaron que no amamantarían a sus bebés si contrajeran COVID-19.Conclusiones: El presente estudio se encontró que muchas de las mujeres encuestadas mostraron un nivel alto de conocimientos, actitudes positivas y prácticas adecuadas relacionadas a lactancia materna.
Dolor articular en relación a la calidad de vida laboral en trabajadores de una empresa prestadora de servicios pecuarios
Authors:   , Izquierdo Loarte Génesis Leslie
Published: November 2024

Objetivo: Determinar la relación entre el dolor articular y la calidad de vida laboral en trabajadores de una empresa prestadora de servicios pecuarios. Método: La investigación posee un diseño cuantitativo, observacional y transversal analítico, cuya variable dependiente es la calidad de vida laboral evaluada con el cuestionario SF-12 y variable independiente, el dolor articular en colaboradores a través del cuestionario Nórdico; aplicando la prueba de Chi cuadrado. Resultado: Participaron 131 trabajadores; 125 varones, la mitad menos de 30 años; el 55% un grado de instrucción nivel secundario o superior; más de la mitad estaba como conviviente; el 74% tenía un IMC más de 25; solo 20 colaboradores tenían un tiempo de trabajo más de 2 años; el 14% solicito algún día de descanso; ningún colaborador realizo una actividad de alto riesgo y más del 70% afirmo realizar actividad física. El 55% refirió algún episodio de dolor en los últimos doce meses de los que en su mayoría se localizó en cuello; casi el 80% afirma tener una calidad de vida laboral buena y de aquellos que tenían dolor la tercera parte presentó mala calidad de vida laboral. Conclusiones: Se halló una asociación entre la presencia de dolor articular en los últimos 12 meses y calidad de vida laboral en trabajadores pecuarios. Siendo progresiva a mayor número de zonas. Las zonas anatómicas más afectadas son la región lumbar, cuello, muñeca, rodilla y tobillo; mas no estuvo asociada con hombros y codo.
Asociación entre uso de pantalla de visualización de datos y síndrome visual informático en trabajadores de una clínica privada de Huaraz
Authors:   , Yengle Milla Yaritza Cristina , Bernuy Canchasto Hans Esteban
Published: November 2024

Objetivo: Determinar la asociación entre el uso de pantalla de visualización de datos y síndrome visual informático en trabajadores de una clínica privada de Huaraz.Material y Método: Investigación observacional, transversal y analítico que incluyó 86 sujetos según cáculo de tamaño muestral. Para la recolección de datos, utilizamos la técnica de la encuesta y como instrumento, un formulario estructurado que constaba de características demográfica, laborales y del uso de pantallas de visualización de datos (PVD). Asi como el uso PVD continua o discontinua y la presencia de síndrome visual informático (SVI). Para hallar asociación, utilizamos el método de Chi cuadrado con un nivel de significanciadel 5%. La investigación tuvo aprobación del Comité de Ética. Resultados: Del total de trabajadores, el 69.8% eran mayores de 35 años, el 72.1% eran varones, el 89.5% procedían de áreas urbanas, el 51.2% se desempeñaban en roles administrativos y el 77.9% tenían menos de 5 años de experiencia laboral. La prevalencia de trabajadores con SVI fue del 26.7%. Además, se evidenció que el uso continuo de PVD se asoció significativamente con el SVI (p<0.001), donde el 53.5% de trabajadores con SVI eran usuarios continuos vs 0% en aquellos con uso discontinuo. Por otro lado, la prevalencia de SVI en trabajadores mujeres fue de 54.2%, de procedencia rural 22.1%, expuestos más de 7 hrs a la PVD 51.7%, con trabajo alternado de PVD 39.2%, uso diario de PVD 41.2%, manejo de computadora de escritorio 46.5%, uso de pantalla fija 40.4% y pantalla al mismo nivel de los ojos 35.8%. Conclusión: El uso continuo de pantallas de visualización de datos se asoció al síndrome visual informático.
Association between endoscopic and radiological activity of inflammatory bowel disease versus dietary inflammatory index and body adiposity
Authors: de Oliveira Rubia , Vasques Ana Carolina , Ribeiro Stefhani , Shivappa Nitin , Hebert James , Nagasako Cristiane
Published: November 2024

Background
Inflammatory Bowel Disease (IBD) has genetic and environmental factors. However, the relationship between activity of IBD, adiposity and dietary pattern has not yet been fully clarified.
Objective
Investigate the association between endoscopic and radiological activity of IBD, body adiposity and dietary inflammatory index (DII).
Method
An observational, cross-sectional study was carried out. Endoscopic activity was defined by: endoscopic Mayo score > 2, Crohn’s Disease Endoscopic Index of Severity (CDEIS) > 5, and/or presence of a deep ulcer in any intestinal segment. Body adiposity was estimated using the body mass index, waist circumference, and waist-hip ratio (WHR). The DII score was calculated from a validated quantitative food frequency questionnaire. According to the DII, the patients were divided into three groups: the first with the less pro-inflammatory diet and the third with a predominantly pro-inflammatory diet.
Results
62 patients were assessed, 58.06% in remission disease (RD) and 41.93% in activity (AD). Overweight/obesity were more frequent in the RD group (p = 0.12); as well as high WHR (p < 0.05) and central obesity (p < 0.01). A predominance of pro-inflammatory diet was observed, both in the AD and RD groups, since most patients were in group 3 in the evaluation of DII and DII adjusted for energy density (E-DII) (p = 0.77 and p = 0.18).
Conclusions
high frequency of a proinflammatory diet and high rates of overweight and obesity were observed in the IBD population, with patients in remission showing higher WHR and central obesity. DII and EDII, were not associated with endoscopic and radiological disease activity in patients with IBD.
Vulnerable and vital: Intermittent estuaries deserve global attention
Authors: Khojasteh Danial , Rao Shivanesh , McSweeney Sarah , Ibaceta Raimundo , Nicholls Robert , French Jon , Glamore William , Largier John , Adams Janine , Hughes Michael , Barry Michael , Power Hannah , Du Jiabi , Tucker Tobias , Cienfuegos Rodrigo , Catalán Patricio , Hanslow David
Published: November 2024

Intermittently open/closed estuaries provide important ecosystem services but are often overlooked in coastal–catchment research and management. These estuaries are highly vulnerable to human/climate disturbances due to their tendency to close off from the ocean, yet their processes/dynamics remain under-researched. This study maps the global distribution of at least 2,245 intermittent estuaries, whose catchments currently support 55 million people, with projections rising to 101 million by 2100. Assessing three decades of scholarly articles indicated that only 7% of these sites have been studied. Academic literature on intermittent estuaries accounted for 0.5% of the total literature on all estuaries, despite these systems representing 4–5% of the estimated total number of global estuaries. Significant research gaps exist in Asia, South America, and Africa, where the largest, most susceptible populations reside. 90% of the existing research on intermittent estuaries is conducted in (southern) Africa (42%), Oceania (35%), and North America (14%), predominantly through domestic efforts. From 1992 to 2023, 60% of the research focused on physio-chemical and eco-hydro-geomorphological topics, with minimal attention to ecosystem services, climatic/human disturbances, and management. Our assessment underscores the need for increased focus on intermittent estuaries and suggests strategies to promote international collaborations, including leadership from intergovernmental organisations.
The Role of Moracin M in Promoting Hair Growth: Insights into Mechanisms of WNT/β- Catenin Pathway Activation and Angiogenesis Enhancement in Human Dermal Papilla Cells
Authors: Chang BoYoon , Hwang Yuri , Kim In , Park Hyungmin , Kim Yonghwan , Kim SungYeon
Published: November 2024

Hair follicle growth depends on the intricate interaction of cells within the follicle and its vascular supply. Current FDA-approved treatments like minoxidil have limitations, including side effects and the need for continuous use. Moracin M, a compound from Moraceae family, was investigated for its effects on hair growth and vascular regeneration. In our study, Moracin M significantly increased cell proliferation in human dermal papilla cells (hDPCs) during both the anagen and catagen phases and promoted cell migration in human umbilical vein endothelial cells (HUVECs) without cytotoxicity at concentrations up to 50 µM. Mechanistic analysis revealed that moracin M enhanced GSK-3β phosphorylation and increased non-phospho β-catenin levels, activating Wnt signaling and upregulating transcription factors LEF, TCF, and AXIN2. This resulted in elevated levels of growth factors VEGF, FGF2, KGF, HGF and MYC in hDPCs, effects comparable to those of minoxidil. Additionally, moracin M significantly increased protein and mRNA levels of VEGF, FGF2, and KGF in hDPCs under IFN-γ-induced inflammatory conditions. Moracin M treatments also resulted in notable wound width reductions in a dose-dependent manner. Further investigation showed that moracin M stimulated MMP-2 and MMP-9 expression while having no effect on MMP-7 levels. These findings indicate that moracin M significantly enhances hair growth through the promotion of cell proliferation and angiogenesis, particularly via the activation of the Wnt signaling pathway in dermal papilla cells, presenting it as a promising therapeutic alternative to current treatments.
Enhanced visual illusions in Parkinson’s disease with neurogenic orthostatic hypotension
Authors: Umehara Tadashi , Mimori Masahiro , Kokubu Tatsushi , Ozawa Masakazu , Shiraishi Tomotaka , Onda Asako , Matsuno Hiromasa , Omoto Shusaku , Murakami Hidetomo , Iguchi Yasuyuki
Published: November 2024

Purpose: Accumulating evidence suggests that sympathetic noradrenergic system degeneration is associated with the pathophysiology of minor hallucination in patients with Parkinson’s disease (PD). We aimed to elucidate the relationship between visual illusion and the extent and type of cardiovascular sympathetic failure in patients with PD.
Methods: The subjects were 133 patients with PD without well-structured visual hallucinations. Visual illusory responses were evaluated using the noise pareidolia test. Cardiac 123I-metaiodobenzylguanidine uptake and neurogenic orthostatic hypotension (nOH) and supine hypertension (nSH) on head-up tilt-table testing were examined in association with the number of illusory responses.
Results: Fifty-one (38%) patients had illusory responses. nOH (β = 0.220, p = 0.008), cognitive impairment (β = −0.228, p = 0.028), and longer symptom duration (β = 0.273, p = 0.006) were associated with greater numbers of illusory responses independently of age, sex, motor severity, levodopa equivalent dose, and anticholinergic and cholinesterase inhibitor use; nSH and peripheral sympathetic degeneration reflected by low cardiac 123I-metaiodobenzylguanidine uptake were not. Although nSH and peripheral sympathetic degeneration had no effects on the association between the number of illusory responses and nOH, patients with severe nOH had significantly more illusory responses than those with mild nOH (p = 0.048).
Conclusion: nOH was associated with the severity of visual illusion in early PD patients without well-structured visual hallucinations, whereas nSH and peripheral sympathetic degeneration were not. These findings deepen our knowledge of the role of sympathetic noradrenergic degeneration in minor hallucinations and may have prognostic implications in patients with early PD.
Satellite remote sensing for the evaluation of the impact of watershed management on surface soil moisture in the Kulfo watershed, Ethiopia
Authors: Tilahun Aklilu Assefa , Zewude Wondafrash Atnafu , Ersuncho Bizuayehu Abera
Published: November 2024

Although watershed management is a valuable strategy for reducing land degradation and increasing surface soil moisture (SSM), quantitative data do not support its effects on watershed hydrology. One of the obstacles to the lack of quantitative evidence of such impacts has been the availability of data. In this work, we showed how useful a remote sensing-based approach is for assessing how SSM in the Kulfo watershed, Ethiopia, is affected by watershed management activities. This study used remotely sensed data (Landsat images) to construct and apply the soil moisture index (SMI) model. The land surface temperature and vegetation index (LST-VI) spatial pixel distribution are interpreted via the trapezoid approach, which forms the basis of the model. From January 2021 to May 2022, we used 42 sample points worth of ground-based moisture measurements to validate the model's performance. Following validation, the surface soil moisture from 1990–2022, including the times before, during, and after watershed improvements were implemented in Kulfo, was examined. The results revealed strong agreement between the SSM predicted by the model and the SSM observed on the ground. This was demonstrated by the low root mean squared error (0.019 cm3 cm− 3) and high R2 (0.81). The surface soil moisture and vegetation cover of the research area increased following extensive physical interventions. Hence, to assess the effects of interventions, a remote sensing approach can detect and quantify SSM. We urge scholars to assess and implement the model for additional watersheds to demonstrate the value of substantial investments in watershed management.
Analyses of the optical and impedance characteristics of solution-casted Sod-Carboxy Methyl Cellulose Biodegradable Polymers prepared with a NaI/KI Blend
Authors: S Nalina , Kumar Sujeet , Bhat Tejashree , V. Prakruthi , S. Keerthana N. , Pattanshetty Seema S. , D Vinay , Potadar Rajeev R. , Putran Suchitra , Prabhu A. N. , Murari M. S. , Pillegowda Manohar , R. Jahnavi K. , Hegde Ganesh Shridhar
Published: November 2024

In the present work, solid polymer films that conduct K+ and Na+ ions were produced using the sodium salt of carboxy methyl cellulose (Na-CMC), poly-ethylene glycol (PEG 600), potassium iodide (KI), and sodium iodide (NaI), and their optical and ionic conductive characteristics were investigated. X-ray diffraction analysis showed that different salt iodides varied in their crystallinity and amorphousness. Various functional groups were verified for visualisation using the Fourier transform infrared spectra technique. Utilising ultraviolet visible spectroscopy, optical analysis was employed to investigate the Urbach energy, direct-band gap, indirect-band gap, dielectric constants, and refractive indices. Ionic conductivity impedance spectroscopy was used to examine the ion dynamics and relaxation processes taking place in the polymer electrolyte.
Targeting TGF-β-Smad2/3-JNK1-mediated SIRT1 activity overcomes the chemoresistance of KRAS mutation lung cancer
Authors: Shin Dong Hoon , Choi Minyoung , Han Chungyong , Kim Sang Soo
Published: November 2024

Lung cancer patients with a KRAS oncogenic driver mutation have a very poor prognosis. Recently, we reported that SIRT1 is upregulated by the KRASMut-c-Myc-axis and KRASMut-induced SIRT1 stably deacetylated by lysine 104 and increased KRASMut activity, enhancing chemoresistance. Of note, SIRT1 activity, as well as SIRT1 levels are more increased in KRASMut cells compared with EGFRMut, both negative cells of KRASMut and EGFRMut, and non-tumorigenic cells. This prompted us to investigate the mechanism by which SRIT1 activity was increased and the role of pSIRT1 in the chemoresistance of KRASMut lung cancer cells. The activated MEK-ERK pathway under KRASMut increased AP-1 transcription activity, which in turn enhanced TGF-β1 secretion. The secreted TGF-β1 activated the Smad2/3-JNK1 signaling pathway through an autocrine manner, increasing pSIRT1S27 and pSIRT1S47, ultimately enhancing KRASMut activity through KRAS deacetylation and affecting chemoresistance. We identified a small molecule from the natural compound library, namely SIRT1 activity inhibitor, Kuwanon C (KWN-C), which decreased pSIRT1S27 and pSIRT1S47 via a decrease in the activity of the TGF-β1-Smad2/3-JNK1 signaling pathway. Treatment with the SIRT1 activity inhibitor triggered the anticancer effects of cisplatin and pemetrexed in human lung cancer cells, lung orthotropic tumors, and in a spontaneous in vivo model of KRASMut lung cancer. Our findings reveal a novel pathway critical for the regulation of SIRT1 activity in KRASMut lung cancer and provide important evidence for the potential application of SIRT1 activity inhibitor as an adjuvant chemotherapy, overcoming chemoresistance in KRASMut lung cancer patients.
The proteome of circulating extracellular vesicles and their functional effect on platelets vary with the isolation method
Authors: Fernández-Sáez Eva M , Bravo Susana B. , Pena Carmen , García Ángel
Published: November 2024

Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and serve as a source of biomarkers in several pathologies. In this study, we aimed to characterize plasma-derived EVs isolated by ultracentrifugation (UC) or size exclusion chromatography (SEC) to define the best method for proteomic and functional studies. EVs characterization included nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), detection of biomarkers by western blotting, and quantitative proteomic analysis. SEC-EVs samples had higher particle and protein concentration, particle-to-protein ratio, and smaller size compared to UC-EVs. A total of 171 proteins were identified through Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) analysis, with 11 increased in SEC-EVs and 5 in UC-EVs. The proteins increased in UC-EVs are complement proteins and immunoglobulins, while proteins increased in SEC-EVs are apolipoproteins and proteins present in the extracellular space. Functional studies with EVs from activated platelets confirmed that EVs isolated by SEC exacerbate platelet aggregation, whereas there was no effect induced by UC-EVs. The latter suggests that EVs obtained by SEC seem more suitable for platelet-related functional studies compared to those obtained by UC. The results presented pave the way for future clinical orientated studies involving plasma-derived EVs.
Alleviating Head-mounted Weight Burden for Neural Imaging in Freely-behaving Rodents Using a Helium-filled Balloon
Authors: Liu Yuehan , Zhang Jing , Li Cheng-Yu , Zhang Haolin , Li Xingde
Published: November 2024

The recently developed miniaturized head-mounted two-photon (2P) imaging devices have served as a valuable tool for neuroscientists, enabling real-time functional neural imaging in freely-behaving animals. Although the current 2P fiberscopes and miniscopes are lightweight, the weight of any potential additional accessories inevitably imposes a burden on the animal. Here, we present a buoyancy levitation method to alleviate head-mounted weight burden on mice. By utilizing the buoyance of a helium-filled balloon to counteract the additional weight of up to 7 g, both the motion behavior and neural activities remain unaffected by the added load. This easy-to-implement method provides a platform for studying neural network function in animals, effectively freeing them from the burden of head-mounted weight.
Image Processing Based Color Analysis of Water Transfer Printed and Ultraviolet Printed Wood Composite Panels
Authors: Budakçı Mehmet , KAÇAMER Serdar , Katırcıoğlu Ferzan
Published: November 2024

In this study, it was aimed to perform color analysis with the Image Processing Based Scrub Tester (IPBST), which was produced to mimic the effect of paint, varnish and various decorative coating layers against household chemicals. For this purpose, 8 mm thick, high-gloss acrylic-coated medium density fiberboard (MDF), polyvinyl chloride (PVC)-coated MDF, and MDF laminate ready-made panels, as well as MDF panels coated with polyurethane, cellulose, acrylic, and water-based paints, were prepared. Carbon fiber patterned decorative coating was applied to the prepared sample panels surfaces using water transfer printing (WTP) and ultraviolet (UV) printing methods. Then, the sample surfaces were scrubbed with IPBST using various household chemicals according to Turkish Standards (TS) EN ISO 11998 principles. In the image processing phase of the study, after taking digital images of the samples before and after the scrubbing process, color measurements were evaluated according to L*, a* and b* coordinates according to the Commission International de I’Eclairage (CIE-Lab) system with a newly developed method. As a result of the research, it was determined that the total color change of the WTP samples was 124.63% more than the UV printed samples. In order to test the accuracy of the color measurement method of the IPBST produced within the scope of the study, a comparison was made with the BYK – Gardner Spektro Guide 45/0 color measurement device. The relationship between the total color change values ​​obtained with both devices was examined by Pearson Correlation analysis, and a very strong and significant relationship of 0.97 was found. As a result, with the similar data obtained, it was proven that the color test analysis performed with IPBST and the software created can be used worldwide and can be an alternative to industrial test devices.
Templating of Monomeric Alpha-Synuclein Induces Inflammation and SNpc Dopamine Neuron Death in a Genetic Mouse Model of Synucleinopathy.
Authors: Byrne Matthew D. , Petramfar Peyman , Lee Jae-Kyung , Smeyne Richard Jay
Published: November 2024

While the etiology of most cases of Parkinson’s disease (PD) are idiopathic, it has been estimated that 5-10% of PD arise from known genetic mutations. The first mutations described that leads to the development of an autosomal dominant form of PD are in the SNCA gene that codes for the protein alpha-synuclein (α-syn). α-syn is an abundant presynaptic protein that is natively disordered and whose function is still unclear. In PD, α-syn misfolds into multimeric b-pleated sheets that aggregate in neurons (Lewy Bodies/neurites) and spread throughout the neuraxis in a pattern that aligns with disease progression. Here, using IHC, HC, HPLC, and cytokine analysis, we examined the sequelae of intraparenchymal brain seeding of pre-formed fibrils (PFFs) and monomeric α-syn in C57BL/6J (WT) and A53T SNCA mutant mice. We found that injection of PFFs, but not monomeric α-syn, into the striatum of C57BL/6J mice induced spread of aggregated α-syn, loss of SNpc DA neurons and increased neuroinflammation. However, in A53T SNCA mice, we found that both PFFs and monomeric α-syn induced this pathology. This suggests that the conformation changes in α-syn seen in the A53T strain can recruit wild-type α-syn to a pathological misfolded conformation which may provide a mechanism for the induction of PD in humans with SNCA duplication/triplication.
Nifuroxazide Improves Inflammation and Septic Shock by Suppressing C/EBPβ Signaling Pathway and Macrophage Migration
Authors: Sun Huifeng , Hu Mingyue , Liu Mei , Wang Min , Guo Jiaying , Xiang Yinhong , Zhang Ning , Liu Jianxin , Jiang Shan
Published: November 2024

Background
Nifuroxazide (NFX) is a broad-spectrum antibacterial agent widely used to treat infectious diarrhea since 1966. In recent years, the discovery of the inhibitory effect of NFX on signal transducer and activator of transcription 3 has prompted extensive research on it as a potential anticancer drug. However, there are very few studies on its anti-inflammatory effects.
Purpose
We want to investigate the mechanism related to anti-inflammation of NFX both in vitro and in vivo.
Study design:
Murine macrophage cells and mouse models for foot swelling mouse models and septic shock were conducted to evaluate the abilities of NFX in anti-inflammation.
Methods
Cell viabilities were evaluated using MTT assay. NO outcome was checked by Griess reagent test. Inflammatory cytokines were determined via ELISA. The mechanism of NFX in anti-inflammation was investigated by immunoblot in protein level. Transwell cell migration assay to detect the effect of NFX on cell migration ability. γ-carrageenan-induced acute foot swelling and septic shock administrated with NFX were studied by H&E and Small-Animal Imaging System.
Results
NFX not only showed no cytotoxicity, but also protected cells from LPS damage. It significantly decreased LPS-induced NO production, but hardly stimulated the expression of NO itself. NFX inhibits CCAAT/enhancer-binding protein β (C/EBPβ) phosphorylation and thereby reduces the production of inflammatory mediators and pro-inflammatory factors such as inducible NO synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). Finally, NFX alleviated γ-carrageenan-induced acute foot swelling and LPS/poly (I:C)-induced septic shock by inhibiting macrophage migration through the C/EBPβ signaling pathway.
Conclusion
In this study, NFX has proven to act as a potent CCAAT/enhancer-binding protein β (C/EBPβ) and macrophage migration inhibitor. NFX is a viable candidate for treating inflammatory diseases, especially for C/EBPβ activation and/or macrophage migration-associated inflammatory diseases.
Biodegradable film based on cellulose nanofiber/methylene blue/vitamin C: investigation of physicochemical properties and usability as a kit for identification of oxidants (hydrogen peroxide)
Authors: Sadeghi Sina , Pirsa Sajad , Asefi Narmela , Gharekhani Mehdi
Published: November 2024

In this study, cellulose nanofiber film was modified with methylene blue pigment and vitamin C (Cel/MB/VC). The physicochemical, structural, thermal resistance, and antibacterial characteristics of the prepared films were investigated using SEM, FTIR, XRD, TGA, etc. The rate of absorption and release of methylene blue from the prepared film was studied. Films containing methylene blue and vitamin C were used as a kit to detect hydrogen peroxide. The obtained results showed that methylene blue and vitamin C increased the thickness of the film and the elongation of the film. Both methylene blue and vitamin C agents reduced moisture content and water vapor permeability. Both methylene blue and vitamin C significantly increased the antioxidant and antimicrobial properties (against Escherichia coli and Staphylococcus aureus) of the film. SEM images showed that the diameter of cellulose fibers is between 20 and 100 nm, which methylene blue and vitamin C completely cover their surface and the surface porosity of the film. FTIR spectra confirmed the electrostatic interactions between cellulose, methylene blue and vitamin C. According to the XRD results, cellulose has a crystalline structure, which vitamin C improved this crystalline property. According to TGA results, methylene blue and vitamin C caused more thermal stability of cellulose film. The release of methylene blue from the film was reported to be 15%. In the presence of hydrogen peroxide, the color of the films containing methylene blue and vitamin C changed from white to blue. Films containing methylene blue and vitamin C at the same time showed good performance as a hydrogen peroxide detection sensor (kit). The highest sensitivity of the sensor for measuring hydrogen peroxide was 0.299 (100 mg/ml) with a detection limit of 1.9 (100 mg/ml). The prepared film has the ability to be used in smart packaging of foods sensitive to oxidation such as oils.
Association of domain-specific physical activity with nocturia: a population- based study
Authors: Jia Yangtao , Shen Rui , Dong Xinke , Yang Fangzheng , Zhou Libin , Long Huimin
Published: November 2024

Background
Nocturia is the most prevalent lower urinary tract symptom (LUTS) and is closely associated with various diseases and social burdens. This study aimed to investigate the relationship between different domains of physical activity (e.g., occupation-related physical activity [OPA], transportation-related physical activity [TPA], and leisure-time physical activity [LTPA]) and nocturia.
Methods
This study included 5516 participants from the cross-sectional National Health and Nutrition Examination Survey (NHANES) conducted between 2007 and 2012. We employed survey-weighted logistic regression, restricted cubic splines (RCS), subgroup analysis, and sensitivity analysis to assess the relationship between different domains of physical activity and nocturia.
Results
Multivariable logistic regression analysis revealed a significant negative correlation between PA, LTPA, and nocturia. Specifically, PA (OR: 0.7523, 95% CI: 0.6307–0.8974, P = 0.002) and LTPA (OR: 0.7664, 95% CI: 0.6314–0.9304, P = 0.007) were negatively associated with nocturia. The RCS curve demonstrated non-linear relationships between PA, LTPA, and nocturia. Additionally, subgroup analyses and sensitivity analyses further validated this association.
Conclusion
Based on this cross-sectional study, we hypothesize that PA and LTPA are associated with a reduced risk of nocturia in adults aged 20 years and older in the United States. This underscoring the importance of physical activity in preventing and managing nocturia may provide valuable guidance for clinical practice.
Risk factors for stoma and incision complications of enterostomy in children with very early-onset inflammatory bowel disease: A prospective cohort study
Authors: Xu Xiaofeng , Zhou Yiwen , Tan Zhixing , Huang Ying , Dong Kuiran , Gu Ying , Chen Jie , Yu Zhuowen
Published: November 2024

Background
Enterostomy is utilized to mitigate severe clinical symptoms in children with very early onset inflammatory bowel disease (VEO-IBD) and to provide a window for stem cell transplantation. Nevertheless, the incidence of post-operative complications is significant, and there is currently a lack of research exploring the risk factors associated with complications related to the stoma and incision following the procedure.
Objective
To investigate the risk factors for stoma and incision complications after enterostomy in patients with VEO-IBD.
Methods
From January 2015 to December 2023, 49 children with VEO-IBD who underwent enterostomy were enrolled in the study. Demographic characteristics, blood biochemical indices, weighted Pediatric Crohn's Disease Activity Index (wPCDAI) and enterostomy-related information were prospectively collected. Multivariate logistic regression was employed to identify the risk factors for ostomy and incision-related complications.
Results
All 49 included VEO-IBD children had interleukin-10(IL-10) signaling defects, with 27 (55.1%) had stomal-related complications and 10 (20.4%) had incision complications after enterostomy. Univariate analysis revealed that wPCDAI (OR: 1.03, 95% CI: 1.00-1.07, P = 0.05) showed a tendency towards statistical significance in the occurrence of ostomy complications. Weight-for-age Z-score(WAZ) (OR: 0.57, 95% CI: 0.39–0.84, P = 0.004), Height-for-age Z-score(HAZ) (OR: 0.57, 95% CI: 0.37–0.88, P = 0.01), type of surgery (OR: 0.12, 95% CI: 0.03–0.56, P = 0.007), C-reactive protein(CRP) (OR: 1.02, 95% CI: 1.01–1.04, P = 0.007), and wPCDAI (OR: 1.08, 95% CI: 1.01–1.14, P = 0.009) demonstrated statistical significance in the occurrence of incision complications. However, multivariate binary logistic regression did not reveal any statistically significant factors.
Conclusion
Although emergency surgery is unavoidable, our study suggests that improving nutritional status, reducing CRP levels, and increasing preoperative wPCDAI scores may help reduce post-enterostomy stoma and incision complications in VEO-IBD children with interleukin-10 (IL-10) signaling defects. Further large-scale studies are needed to confirm these findings.
Genetic analysis of Poirier Bienvenu neurodevelopmental syndrome associated with CSNK2B variation
Authors: Yang Liu , Mei Daoqi , Liu Yanping , Gao Li
Published: November 2024

CSNK2B deficiency underlies the pathogenesis of Poirier-Bienvenu neurodevelopmental syndrome (POBINDS). In this study, we present four cases of pediatric seizures caused by de novo variants in CSNK2B, with the aim to reinforce the clinical and variant data pertaining to early genetic factors associated with epilepsy. Methods: Trio whole exome sequencing were used to detect variations in the proband and her family members, and bioinformatics annotation was performed for the variation. Sanger sequencing and CSNK2B cDNA sequencing were employed to ascertain the carrier status of additional family members and evaluate the potential impact of variants on splicing. Results: All four children presented with epilepsy as the initial manifestation, accompanied by mild-moderate mental retardation, language and motor developmental delay. Cases 1, 3 and 4 exhibited full-scale tonic-clonic seizures, while case 2 displayed myoclonic and typical absence seizures. Furthermore, case 2 demonstrated delayed growth and development compared to age-matched peers. No abnormality was detected in the head magnetic resonance imaging (MRI). Genetic analysis revealed novel heterozygous variations in the CSNK2B gene in all four children, including c.175 + 1G > A, c.73-2A > G, c.291 + 1G > A and c.481delA. In case 2, reverse transcription analysis of CSNK2B mRNA revealed the retention of the 3' end sequence of Intron 2 and deletion of the 5' end sequence of Exon 3. In treatment, four children received a combination of one to three types of antiepileptic drugs and rehabilitation training individually. Case 1 continued to experience seizures to varying degrees, while cases 2–4 demonstrated effective seizure control. Overall motor and intellectual development improved in all four children, however, there was slow recovery in language function. Conclusion: This study elucidates the molecular etiology of epilepsy in four patients with POBINDS and expands the mutational spectrum of pathogenic variants in the CSNK2B, highlighting their impact on splicing. The highly genetic heterogeneous phenotype of POBINDS relies on the detection of pathogenic variants in CSNK2B. Conventional antiepileptic drugs effectively control seizures, while rehabilitation treatment can significantly improve intelligence and motor function to varying degrees; however, language recovery tends to be relatively slow.
Proximal humerus head split fractures in young adults- Outcomes of internal fixation and whether we can afford to miss them
Authors: PRASHANTH TARUN , MUKHERJEE KAUSTAV , KUMAR MOHAN , RAJA DORAI KUMAR , RAJA SUJITH KUMAR
Published: November 2024

Purpose
Humerus head split fractures are rare subgroup of proximal humerus fractures and are often challenging to treat. We aim to bring forward the difficulties in identifying the isolated head split fractures, modes of internal fixations, possible complications of missing the head split component in a complex proximal humerus fracture fixation and the outcomes on the functionality of the shoulder joint.
Materials and Methods
A retrospective case-control study, 2010 to 2020, with all fractures treated by internal fixation, was carried out at our institution. Fixation was carried out on basis of surgeon’s preference- a sub-group with cancellous screws alone, while some patients were fixed with plate-augmented-screw construct. Control group included cases where head splits were not addressed appropriately. Functional and radiological analysis were done at regular follow-up visits, over the next 12 months.
Results
A total of 24 patients were included in the study- 9 fixed by screws alone and 15 by plate-augmented-screw construct. The mean age of the study group was 34.5 years with 85% male patients. Bony union was achieved in all cases with average time to union being 29 weeks. Study group recorded mean Oxford shoulder score 49, contrary to the control group with mean score 22. Out of 10 patients in the control group- 3 cases of avascular necrosis and 2 cases of nonunion were recorded.
Conclusion
Simple head splits are easily missed on standard shoulder radiographs and needs additional imaging/views for diagnosis. Schiebel type 1 and 2 fractures recorded excellent functional outcomes when addressed appropriately.
Use of PULSAR (Personalized Ultra-fractionated Stereotactic Adaptive Radiotherapy) as consolidation with immune checkpoint inhibition in the treatment of pediatric metastatic melanoma
Authors: McCarty Kyra L. , Watt Tanya , Dan Tu D. , Timmerman Robert D. , Kumar Kiran A.
Published: November 2024

We present a case of extensive and bulky pediatric metastatic melanoma originating in the head and neck which markedly responded to combination therapy with anti-programmed cell death (PD-1) inhibition and consolidative personalized ultra-fractionated stereotactic adaptive radiotherapy (PULSAR). After surgical debulking with neck dissection, the patient was initially treated with anti-PD-1 and anti-cytotoxic T-lymphocyte associated protein 4 (CTLA-4) dual checkpoint blockade immunotherapy, but quickly had disease progression. He was transitioned to a different anti-PD-1 immunotherapy in combination with B rapidly accelerated fibrosarcoma (BRAF) and methyl ethyl ketone (MEK) tyrosine kinase inhibitors in conjunction with consolidative local therapy using PULSAR. This combination therapy achieved tumor response and progression-free status for one year followed by further disease progression at a separate site in the mediastinum. Due to otherwise good disease control, single agent anti-PD-1 immunotherapy was continued, and salvage PULSAR was administered to the progressive site, again resulting in tumor response and progression-free status for 6 months. None of the bulkier sites of gross disease had local progression after combination therapy. This case suggests that the synergistic effect of PULSAR and anti-PD-1 immunotherapy is efficacious for relapsed or refractory metastatic melanoma in pediatric patients. Clinical trial number: not applicable.