bluuebunny/crossref_metadata_2025 huggingface dataset:
Simulation and Study of Manufacturing of W–Cu Functionally Graded Materials by a Selective Laser Melting Process
Authors: Ding Xiaoyu , Ma Di , Fu Yuecheng , Luo Laima , Wu Yucheng , Yao Jianhua
Published: December 2024

Plasma-facing components (PFCs) were simulated by ANSYS, and the influence of gradient layer number and composition distribution index on the distribution of temperature field and stress field was analyzed. The simulation results show that a gradient structure with four gradient layers and a component distribution index of 1 makes the PFC assembly have lower overall temperature and lower thermal stress. Tungsten–copper functionally graded materials (W–Cu FGMs) (W-20 vol% Cu/W-40 vol% Cu/W-60 vol% Cu/W-80 vol% Cu) were fabricated by a selective laser melting (SLM) process based on finite element simulation results. The effects of microstructure on the hardness, internal stresses, thermal conductivity, and thermal expansion coefficient of the W–Cu FGMs were evaluated. The results show that hardness increases from 196 to 1173 HV0.3 with increasing W content. The internal stresses of W-20 vol% Cu, W-40 vol% Cu, W-60 vol% Cu, and W-80 vol% Cu are about 191.7 MPa, 627 MPa, 1049.5 MPa, and 561.9 MPa, respectively. The thermal conductivity of the W–Cu FGM is 23 W/m·K and the thermal diffusion coefficient is 10 mm2/s at 25 °C, and the thermal conductivity rises to 70 W/m·K and the thermal diffusion coefficient rises to 18.5 mm2/s at 800 °C. After 100 thermal shock cycles, the internal defects increased, but the interface between the gradient layers remained well bonded.
Analysis of Glauconite Research Trends Based on CiteSpace Knowledge Graph
Authors: Nong Ke , Chen Si , Ren Zepeng , Zeng Min
Published: December 2024

This paper aims to explore the current status and development trend of glauconite research through CiteSpace (version 6.2.R3) software tools. Based on the literature records from 1980 to 2024 in the Web of Science Core Collection database, this study visualizes the countries/regions, institutions, authors, journals, literature, and keywords related to glauconite. The results show that the United States, Russia, India, France, and England are the main contributing countries to glauconite research, the Russian Academy of Sciences is the institution with the largest number of publications, and Santanu Banerjee and Gilles S. Odin are the most influential authors. The field of glauconite has a high degree of international cooperation and multidisciplinary cross-disciplinary nature. The research hotspots of glauconite are also identified, including origin, basin, sediments, geochemistry, sandstone, and climate, and emerging research frontiers such as fertilizer, removal, provenance, composition characteristics, and Fe are pointed out. Glauconite research is not only of great significance in the field of geology, but its application potential in environmental management and agricultural development is also gradually being recognized, indicating that this field has broad research and application prospects in the future.
Diagenetic Controls of Sandstone Densification in the Lower Cretaceous Laiyang Group, Lingshan Island, Eastern China
Authors: Chen Tongtong , Zhou Yaoqi , Liu Hanqing , Liu Ruiyang
Published: December 2024

The Lower Cretaceous Laiyang Group on Lingshan Island contains typical gas source and clastic reservoir rocks. The densification mechanism of clastic rock and its diagenetic connection have not been systematically studied, which significantly increases the risk associated with hydrocarbon exploration in eastern China. A comprehensive study was conducted on core samples obtained from the Scientific Drilling Borehole LK-1, utilizing core data in conjunction with a range of techniques, including microscopic observation, X-ray diffraction, physical property measurements, and low-temperature nitrogen adsorption. The results indicate that the sandstones are primarily composed of feldspathic litharenite, exhibiting a poorly to moderately sorted texture and a fine-to-medium grain size. The reservoir quality is quite poor, characterized by extremely low porosity and permeability. The reservoir space of tight sandstones is constituted by three main types of pores: residual primary pores, secondary dissolution pores, and intercrystalline pores. Tight sandstone reservoirs experienced notable diagenetic alteration during burial, with calcite, dolomite, quartz, and clay cements identified as the primary diagenetic minerals. Intense compaction and carbonate cementation are the principal mechanisms contributing to the densification of sandstones. Pore-filling clay minerals subdivide macropores into numerous micropores, significantly reducing reservoir permeability. The migration of dissolution products out of the system is a difficult process, which hinders the effectiveness of mineral dissolution in enhancing overall reservoir quality. This study may provide a valuable reference for the effective exploration of Lower Cretaceous clastic reservoirs in eastern Shandong.
Studying the Effect of Reducing Agents on the Properties of Gold Nanoparticles and Their Integration into Hyaluronic Acid Hydrogels
Authors: Adamska Elżbieta , Kowalska Agata , Wcisło Anna , Zima Katarzyna , Grobelna Beata
Published: December 2024

Gold nanoparticles (Au NPs) are a promising target for research due to their small size and the resulting plasmonic properties, which depend, among other things, on the chosen reducer. This is important because removing excess substrate from the reaction mixture is problematic. However, Au NPs are an excellent component of various materials, enriching them with their unique features. One example is hydrogels, which provide a good, easily modifiable base for multiple applications such as cosmetics. For this purpose, various compounds, including hyaluronic acid (HA) and its derivatives, are distinguished by their high water-binding capacity and many characteristics resulting from their natural origin in organisms, including biocompatibility, biodegradability, and tissue regeneration. In this work Au NPs were synthesized using a green chemistry method, either by using onion extract as a reductant or chemically reducing them with sodium citrate. A complete characterization of the nanoparticles was carried out using the following methods: Fourier-Transform Infrared Spectroscopy (FT-IR), Electrophoretic (ELS), and Dynamic Light Scattering (DLS) as well as Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Their antioxidant activity was also tested using the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The results showed that the synthesized nanoparticles enrich the hydrogels with antioxidant properties and new surface properties (depending on the reducing agent, they can be more hydrophilic or hydrophobic). Preliminary observations indicated low cytotoxicity of the nanomaterials in both liquid form and as a hydrogel component, as well as their lack of penetration through pig skin. The cosmetic properties of hydrogel masks were also confirmed, such as increasing skin hydration.
Revitalization of Soil Contaminated by Petroleum Products Using Materials That Improve the Physicochemical and Biochemical Properties of the Soil
Authors: Wyszkowska Jadwiga , Borowik Agata , Zaborowska Magdalena , Kucharski Jan
Published: December 2024

One of the key challenges in environmental protection is the reclamation of soils degraded by organic pollutants. Effective revitalization of such soils can contribute to improving the climate and the quality of feed and food, mainly by eliminating harmful substances from the food chain and by cultivating plants for energy purposes. To this end, research was carried out using two sorbents, vermiculite and agrobasalt, to detoxify soils contaminated with diesel oil and unleaded gasoline, using maize as an energy crop. The research was carried out in a pot experiment. The level of soil contamination with petroleum products was set at 8 cm3 and 16 cm3 kg−1 d.m. of soil, and the dose of the revitalizing substances, i.e., vermiculite and agrobasalt, was set at 10 g kg−1 of soil. Their effect was compared with uncontaminated soil and soil without sorbents. The obtained research results prove that both diesel oil and gasoline disrupt the growth and development of Zea mays. Diesel oil destabilized plant development more than gasoline. Both products distorted the activity of soil oxidoreductases and hydrolases, with diesel oil stimulating and gasoline inhibiting. The applied sorbents proved to be useful in the soil revitalization process, as they reduced the negative effects of pollutants on Zea mays, increased the activity of soil enzymes, enhanced the value of the biochemical soil quality indicator (BA), and improved the cation exchange capacity (CEC), the sum of exchangeable base cations (EBC), pH, and the Corg content. Agrobasalt demonstrated a greater potential for improving soil physicochemical properties, inducing an average increase in CEC and EBC values of 12% and 23%, respectively, in soil under G pressure, and by 16% and 25% in DO-contaminated soil.
Enhancement of the Thermostability of Microbacterium Esterase by Combinatorial Rational Design
Authors: Peng Wenyu , Wu Xiaomei , Ma Baodi , Xu Yi
Published: December 2024

The esterase EstSIT01 from Microbacterium can catalyze the asymmetric hydrolysis of meso-dimethyl ester to produce the crucial chiral intermediate (4S, 5R)-hemimethyl ester for d-biotin synthesis. Despite its high yields and stereoselectivity, the low thermostability of EstSIT01 limits its practical application. Herein, two kinds of rational strategies were combined to enhance the thermostability of EstSIT01. Based on the Surface Residue Substitution (SRS) method, two variants (G215A and G316A) with improved thermostability and one mutant (G293A) with superior activity were identified from nine candidates. According to the Consensus Mutation method, two mutants (E301P and A332P) with enhanced thermostability were identified from six candidates. However, the combined mutation failed to yield mutants surpassing the best single mutant, E301P, in terms of thermostability. The combined mutant E301P/G215A and E301P/G215A/G293A exhibited a slight enhancement in enzyme activity relative to E301P, while also exhibiting improved thermostability compared to the wild-type EstSIT01. Compared with the wild-type esterase, the thermal inactivation half-lives (t1/2) of mutant E301P were enhanced 1.4-fold, 2.4-fold and 1.8-fold at 45 °C, 55 °C, and 65 °C, respectively. The optimal reaction temperature and pH for mutant E301P remained consistent with those of the wild type, at 40 °C and 10.0, respectively. The Km of E301P was 0.22 ± 0.03 mM and the kcat was 5.1 ± 0.28 s−1. Further analysis indicated that the free energies of G215A, G293A and E301P were decreased by 0.91, 0.308 and 1.1049 kcal/mol, respectively, compared to the wild-type EstSIT01. The interaction analysis revealed that the substitution of glutamic acid with proline at position 301 enhanced the hydrophobic interactions within the protein. The decreased free energies and the increased hydrophobic interactions were well correlated with the enhanced stability in these mutants.
Research on the Adsorption Mechanism and Performance of Cotton Stalk-Based Biochar
Authors: Cui Qiushuang , Huang Yong , Ma Xufei , Li Sining , Bai Ruyun , Li Huan , Liu Wen , Wei Hanyu
Published: December 2024

In this research, we produced two types of biochar (BC) using cotton stalks as raw material and KOH as an activator, and compared their performance and adsorption mechanisms in the removal of tetracycline (TC) and methylene blue (MB) from wastewater. The results showed that the biochar generated using both procedures formed pores that connected to the interior of the biochar and had extensive microporous and mesoporous structures. The molten salt approach produces biochar with a higher specific surface area, larger pore size, and higher pore volume than the impregnation method, with a maximum specific surface area of 3095 m2/g. KBCM-900 (the BC produced using the molten salt method at 900 °C) had a better adsorption effect on TC, with a clearance rate of more than 95% in 180 min and a maximum adsorption amount of 912.212 mg/g. The adsorption rates of the two BCs for MB did not differ significantly at low concentrations, but as the concentration increased, KBCI-900 (the BC generated by the impregnation method at 900 °C) exhibited better adsorption, with a maximum adsorption of 723.726 mg/g. The pseudo-second-order kinetic model and the Langmuir isotherm model may accurately describe the TC and MB adsorption processes of KBCI-900 and KBCM-900. The KBCI/KBCM-900 adsorption process combines physical and chemical adsorption, with the primary mechanisms being pore filling, π–π interactions, hydrogen bonding, and electrostatic interactions. As a result, biochar generated using the molten salt method is suitable for the removal of large-molecule pollutants such as TC, whereas biochar prepared using the impregnation method is suitable for the removal of small-molecule dyes such as MB.
Bioassay-Guided Isolation and Identification of Antibacterial Compounds from Invasive Tree of Heaven Stem and Trunk Bark
Authors: Cselőtey Anna , Baglyas Márton , Király Nóra , Ott Péter G. , Glavnik Vesna , Vovk Irena , Móricz Ágnes M.
Published: December 2024

Flash column chromatographic fractionation of tree of heaven (Ailanthus altissima) stem and trunk bark extracts, guided by thin-layer chromatography (TLC)–Bacillus subtilis assay and TLC–heated electrospray high-resolution tandem mass spectrometry (HESI-HRMS/MS), lead to the isolation of six known compounds: (9Z,11E)-13-hydroxy-9,11-octadecadienoic acid (13-HODE, A1), (10E,12Z)-9-hydroxy-10,12-octadecadienoic acid (9-HODE, A2), hexadecanedioic acid (thapsic acid, A3), 16-hydroxyhexadecanoic acid (juniperic acid, A4), 16-feruloyloxypalmitic acid (alpinagalanate, A5), and canthin-6-one (A6). Their structures were elucidated by HESI-HRMS/MS and one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. This is the first study identifying A1–A5 in A. altissima tree. Except for A5, all isolated compounds exhibited antibacterial activity against B. subtilis in microdilution assays. A6 showed the strongest effect with a minimum inhibitory concentration (MIC) value of 8.3 µg/mL. The antibacterial activity of A3 and A4 is newly described.
Study on pH-Responsive Delayed, Cross-Linking and Weighted Fracturing Fluid
Authors: Bai Hao , Zhou Fujian , Liu Xinlei , Xin Xiaozhi , Zhao Huimin , Ding Zhiyuan , Wang Yunjin , Wang Xin , Li Xingting , Li Wei , Yao Erdong
Published: December 2024

Hydraulic fracturing of deep, high-temperature reservoirs poses challenges due to elevated temperatures and high fracture pressures. Conventional polymer fracturing fluid (QCL) has high viscosity upon adding cross-linking agents and significantly increases wellbore friction. This paper examines a polymer fracturing fluid with pH response and low friction. Experimental results indicate that cross-linking occurs quickly in acid, while alkali can slow the cross-linking process and reduce friction. Sodium carbonate (Na2CO3) serves as an effective candidate. An optimized formulation consisting of “salt + pH + polymer + cross-linking agent” is proposed in two stages: low viscosity for fracture generation and high viscosity for sand transport. PH control enhances polymer hydration, increasing sand-carrying in the low-viscosity stage. Scanning electron microscopy (SEM) reveals that the fluid’s structure varies with pH, showing that alkali promotes a stable network structure. Infrared spectroscopy (IR) shows that higher pH increases negative charges of the polymer chains, which enhances their hydrodynamic radius, slightly raises viscosity, and enhances sand carrying. Field tests confirm the formulation’s effectiveness, leading to lower operating pressures, stable sand transport, and notable production, averaging 107.57 m3 of oil and 276 m3 of gas per day. Overall, this research provides low-friction solutions for the efficient development of deep reservoirs.
Synthesis of Oxazoles Containing CF3-Substituted Alcohol Unit via Tandem Cycloisomerization/Hydroxyalkylation from N-Propargylamides with Trifluoropyruvates
Authors: Gao Juan-Juan , Wu Long-Hui , Yu Shu-Qin , Zhu Xue , Zeng Yu , Yang Kai , Wang Zhao-Yang
Published: December 2024

Oxazoles are important five-membered heterocycles that contain both nitrogen and oxygen atoms. Due to their wide range of biological activities, many oxazoles demonstrate potential for extensive application in various fields, including medicinal chemistry. Trifluoromethyl carbinol, an important pharmacophore, contains both trifluoromethyl and hydroxyl groups and is common in molecules with important biological activities. Constructing oxazoles that contain a trifluoromethyl carbinol unit is undoubtedly important and valuable for expanding the chemical space in drug discovery. In this study, a simple and efficient method was developed for the synthesis of oxazoles containing a CF3-substituted alcohol unit via the tandem cycloisomerization/hydroxyalkylation of N-propargylamides with trifluoropyruvates through a rational Lewis acid catalytic mechanism. This Zn(OTf)2-catalyzed synthetic protocol is operationally simple and provides a series of oxazoles in moderate to good yields. The protocol demonstrates broad substrate scope, high functional group tolerance, and high atom economy and can achieve gram-level reactions, indicating the strong possibility of its practical application.
Impact of Thermal, High-Pressure, and Pulsed Electric Field Treatments on the Stability and Antioxidant Activity of Phenolic-Rich Apple Pomace Extracts
Authors: Plamada Diana , Arlt Miriam , Güterbock Daniel , Sevenich Robert , Kanzler Clemens , Neugart Susanne , Vodnar Dan C. , Kieserling Helena , Rohn Sascha
Published: December 2024

Apple pomace, a by-product of apple juice production, is typically discarded as waste. Recent approaches have focused on utilizing apple pomace by extracting beneficial bioactive compounds, such as antioxidant phenolic compounds (PCs). Before these PC-rich extracts can be used in food products, they must undergo food preservation and processing methods. However, the effects of these processes on the composition, stability, and properties of the PC remain insufficiently understood. The present study aimed at investigating the effects of a thermal treatment (TT), a high-pressure thermal treatment (HPTT), and a pulsed electric field treatment (PEF) on the composition and antioxidant activity of PC-rich apple pomace extracts (APEs). Major PCs, including phloridzin, chlorogenic acid, and epicatechin, as well as minor compounds, were identified by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and high-performance thin-layer chromatography (HPTLC). As a stability indicative property, the antioxidant activity was analyzed by a Trolox equivalent antioxidant capacity assay (TEAC), electron paramagnetic resonance spectroscopy, and the Folin–Ciocalteu reagent assay. The results showed that TT at 80 °C increased phloridzin content, likely due to the hydrolysis of bound forms, while higher temperatures and HPTT resulted in a substantial PC conversion. The PEF treatment also caused notable PC conversion, but generally, it had a milder effect compared to TT and HPTT. Hence, low temperatures with and without high pressure and PEF seem to be the most promising treatments for preserving the highest content of major PC in APE. Antioxidant activity varied among the analytical methods, with HPTT showing minor changes despite PC loss compared to the untreated APE. This suggests that other antioxidant compounds in the extracts may contribute to the overall antioxidant activity. This study demonstrates that apple pomace contains valuable PC, highlighting its potential as a health-promoting food additive and the impact of conventional preservation and processing methods on PC stability.
The Trapping Mechanism at the AlGaN/GaN Interface and the Turn-On Characteristics of the p-GaN Direct-Coupled FET Logic Inverters
Authors: Yu Junfeng , Ding Jihong , Wang Tao , Huang Yukai , Du Wenzhang , Liang Jiao , Ma Hongping , Zhang Qingchun , Li Liang , Huang Wei , Zhang Wei
Published: December 2024

The trapping mechanism at the AlGaN/GaN interface in the p-GaN high electron mobility transistors (HEMTs) and its impact on the turn-on characteristics of direct-coupled FET logic (DCFL) inverters were investigated across various supply voltages (VDD) and test frequencies (fm). The frequency-conductance method identified two trap states at the AlGaN/GaN interface (trap activation energy Ec-ET ranges from 0.345 eV to 0.363 eV and 0.438 eV to 0.47 eV). As VDD increased from 1.5 V to 5 V, the interface traps captured more electrons, increasing the channel resistance (Rchannel) and drift-region resistance (Rdrift) of the p-GaN HEMTs and raising the low-level voltage (VOL) from 0.56 V to 1.01 V. At fm = 1 kHz, sufficient trapping and de-trapping led to a delay of 220 µs and a VOL instability of 320 mV. Additionally, as fm increased from 1 kHz to 200 kHz, a positive shift in the threshold voltage of p-GaN HEMTs occurred due to the dominance of trapping. This shift caused VOL to rise from 1.02 V to 1.40 V and extended the fall time (tfall) from 153 ns to 1 µs. This investigation enhances the understanding of DCFL GaN inverters’ behaviors from the perspective of device physics on power switching applications.
Effects of Zinc-Biofortified Wheat Intake on Plasma Markers of Fatty Acid Metabolism and Oxidative Stress Among Adolescents
Authors: Shahzad Babar , Holt Roberta R. , Gupta Swarnim , Zaman Mukhtiar , Shahzad Muhammad , Lowe Nicola M. , Hall Andrew G.
Published: December 2024

Background/Objective: Zinc deficiency is common worldwide and has been linked to reduced growth and immune function, increased risk of and slower recovery from infections, and increased risk of non-communicable diseases. To address the issue, zinc biofortification of wheat has been proposed as a sustainable approach to increase dietary zinc intake in countries like Pakistan, where zinc deficiency rates are high and wheat is the primary staple crop. Since plasma zinc concentration (PZC) does not reliably respond to small changes in zinc intake, biomarkers sensitive to small changes in zinc intake achievable though biofortification are needed. Activity indices for zinc-dependent metabolic steps of desaturation and elongation of omega-6 fatty acids (FAs) have been proposed as sensitive zinc biomarkers. Oxylipin metabolites of polyunsaturated FAs may also respond to changes in zinc intake and further mediate metabolic response to oxidative stress. The objective of the current study was to assess the effects of consuming zinc-biofortified wheat flour on plasma markers of fatty acid (FA) metabolism in females aged 10–16 years. Methods: A nested secondary analysis was conducted in samples from a double-blind, cluster-randomized controlled trial conducted in rural Pakistan, whereparticipants (n = 517) consumed either zinc-biofortified wheat flour or control flour for 25 weeks. Total plasma FAs and oxylipins were measured by liquid chromatography tandem mass spectrometry (LC–MS/MS). Activity indices were estimated from the ratios of product to precursor FAs. Results: Except for docosahexaenoic acid (DHA, p < 0.05), no significant intervention effect was observed on plasma FAs and FA activity index endpoints. Zinc-biofortified wheat intake reduced pro-inflammatory oxylipins and biomarkers of oxidative stress, 5-HETE (p < 0.05), 9-HETE (p < 0.05), 11-HETE (p < 0.05), and 15-HETE (p < 0.05), compared with the control. However, after adjustment for multiple comparisons, none of the intervention effects remained significant. Conclusions: Further study of the responsiveness and specificity of plasma oxylipins to changes in zinc intake is warranted.
Lutein Maintains Bone Mass In Vitro and In Vivo Against Disuse-Induced Bone Loss in Hindlimb-Unloaded Mice
Authors: Tanaka Yuki , Tominari Tsukasa , Takatoya Masaru , Arai Daichi , Sugasaki Moe , Ichimaru Ryota , Miyaura Chisato , Matsumoto Chiho , Ma Sihui , Suzuki Katsuhiko , Hirata Michiko , Grundler Florian M. W. , Inada Masaki
Published: December 2024

Background: Lutein, a carotenoid, exhibits various biological activities such as maintaining the health of the eye, skin, heart, and bone. Recently, we found that lutein has dual roles in suppressing bone resorption and promoting bone formation. In this study, we examined the effects of lutein in a disuse-induced osteoporosis model using hindlimb-unloaded (HLU) mice. Methods: Osteoclast differentiation was assessed by coculturing mouse primary osteoblasts and bone marrow cells or culturing a mouse osteoclast precursor cell line. The bone-resorbing activity was determined by mouse calvarial organ cultures. An in situ docking simulation was conducted to reveal the interaction of lutein and IκB kinase (IKK) β protein. HLU mice were fed a 1% lutein-containing diet for two weeks, and the femoral bone mass was measured by μCT. Results: Osteoclast differentiation is significantly inhibited by lutein, astaxanthin, and β-cryptoxanthin. In contrast, only lutein promoted osteoblastic calcified bone nodule formation. To elucidate the molecular role of lutein, we functionally analyzed the NF-κB complex, a molecule involved in bone metabolism, especially in osteoclasts. Docking simulations showed that lutein binds to IKK, thus inhibiting the activation of NF-κB. In a cell culture analysis, the phosphorylation of p65, the active form of NF-κB in osteoblasts, was suppressed by lutein treatment. In vivo, a μCT analysis of the bone microarchitecture showed that lutein improves several bone parameters while maintaining bone mass. Conclusions: Lutein is effective in maintaining bone mass by controlling both bone resorption and formation, which is applied to prevent disuse-induced osteoporosis.
Anticancer Activity of Plant Tocotrienols, Fucoxanthin, Fucoidan, and Polyphenols in Dietary Supplements
Authors: Lara-Hernández Gabriel , Ramos-Silva José Alberto , Pérez-Soto Elvia , Figueroa Mario , Flores-Berrios Ericka Patricia , Sánchez-Chapul Laura , Andrade-Cabrera José Luis , Luna-Angulo Alexandra , Landa-Solís Carlos , Avilés-Arnaut Hamlet
Published: December 2024

Background: Plants and algae harbor diverse molecules with antioxidant activity and have been demonstrated to directly inhibit cancer cell growth and mitigate the oxidative damage associated with certain antitumor therapies. While antioxidant supplementation, either alone or in combination with chemotherapy, has shown promise in improving quality of life, further research is needed to explore the effects of antioxidant combinations on specific cancer cell lines. Methods: In this study, the in vitro cytotoxic and apoptotic properties of natural compounds derived from plants and algae, as well as certain dietary supplements, were investigated against various human cancer cell lines, including bone, leukemia, colorectal, breast, and prostate cancers. Results: Apple polyphenols, fucoxanthin, and plant-derived tocotrienols exhibited cytotoxic effects across all lines; however, tocotrienols demonstrated the most potent, time-dependent cytotoxic activity, with a half-inhibitory concentration (IC50) of 4.3 μg/mL in bone cancer cells. Analysis of dietary supplements 2.1, 4.0, and 10.0 revealed that supplement 10.0 exhibited specific cytotoxic activity against bone cancer line TIB-223 and colorectal cancer cell line Caco2, with IC50 values of 126 μg/mL and 158 μg/mL, respectively. Both tocotrienols and supplement 10.0 induced morphological changes in TIB-223 cells, inhibited cell migration (anti-metastatic activity), and promoted apoptosis, as evidenced by caspase 3/7 activation in both bone and colorectal cancer cells. Conclusions: These findings provide valuable insights for the development of targeted dietary supplements to enhance the anticancer effect of conventional chemotherapy in specific cancer types.
Assessing Quality of Life with the Novel QLQ-CAX24 Questionnaire and Body Composition Parameters in Rectal Cancer Patients: A Single-Center Prospective Study
Authors: Cintoni Marco , Palombaro Marta , Raoul Pauline , Chiloiro Giuditta , Romano Angela , Meldolesi Elisa , De Giacomo Flavia , Leonardi Elena , Egidi Gabriele , Grassi Futura , Pulcini Gabriele , Rinninella Emanuele , Capristo Esmeralda , Gasbarrini Antonio , Gambacorta Maria Antonietta , Mele Maria Cristina
Published: December 2024

Background: Patients with rectal cancer (RC) are at risk of developing cancer-related cachexia, a complex metabolic syndrome that can negatively impact quality of life (QoL), treatment tolerance, and clinical response. Objectives: The aim of the study was to explore the possible associations of the novel European Organization for Research and Treatment of Cancer QoL Questionnaire—Cancer Cachexia (EORTC QLQ-CAX24) scores with body composition parameters and physical performance in patients with locally advanced RC (LARC). Methods: This prospective observational study involved RC patients evaluated at the dedicated outpatient clinic of Clinical Nutrition at the Fondazione Policlinico Agostino Gemelli IRCCS. Patients with a confirmed diagnosis of LARC were enrolled between January and December 2023. The body composition parameters were measured using the preoperative computed tomography scan at the level of the third lumbar vertebra as well as using bioimpedance analysis before and after the radiotherapy treatment. QoL was measured by the EORTC QLQ-C30 and EORTC QLQ-CAX24 questionnaires. Results: A total of 56 RC patients were enrolled. Significant associations (p < 0.05) were found between EORTC QLQ-CAX24 values and the presence of cachexia, body composition, handgrip strength, and malnutrition diagnosis. Muscle mass was significatively also associated with EORTC QLQ-CAX24 results, suggesting a link between subjective perception of QoL and objectively measured body composition. Conclusions: The EORTC CAX24 questionnaire can be an effective tool for monitoring changes in cachexia status during radiotherapy, enabling early detection of cachexia-related complications and timely intervention.
The Acacia (Vachellia nilotica (L.) P.J.H. Hurter & Mabb.): Traditional Uses and Recent Advances on Its Pharmacological Attributes and Potential Activities
Authors: Hafez Lamiaa O. , Brito-Casillas Yeray , Abdelmageed Noha , Alemán-Cabrera Isabel M. , Morad Samy A.F. , Abdel-Raheem Mahmoud H. , Wägner Ana M.
Published: December 2024

For thousands of years, Vachellia nilotica has been widely used as an herbal medicine to treat some diseases and symptoms, including respiratory, gastrointestinal and urogenital ailments. The present study was adapted to document and assemble existing information about V. nilotica and its evidence-based ethnopharmacological activities, with brief reviews on the description, geographical distribution, ecology, medical uses and phytochemistry. A literature review and information up to 2024 was performed in various scientific databases, including PubMed, Science Direct and Google Scholar. The keywords were “Acacia nilotica”, “Botany”, “ecology”, “Traditional uses”, “Phytochemistry”, “Polyphenols”, “Molecular docking”, “Ethnopharmacological activities” and “toxicity”, among others. V. nilotica has a wide range of uses, with low toxicity, reported in different countries. It can be infused into oils or tea or incorporated into paste, poultice and biscuits, used as an emollient, antidiarrheal, astringent and as an antidote for bite poisons. Glucose and lipid-lowering, anti-inflammatory, analgesic, antipyretic, antioxidant, antihypertensive, antibacterial, antifungal, antiviral and anthelmintic activities are the most prominent. Over 150 chemical components have been identified from V. nilotica that could be associated with its potential actions. Quercetin, rutin, kaempferol, naringenin, catechin, epicatechin, gallic acid, ellagic acid, lupeol and niloticane are its main active constituents. From the research data, and despite the fact that human clinical trials and detailed methodological studies are scarce, V. nilotica has shown wide-ranging activities, though the most robust evidence is related to the treatment of microbial infections, diarrhea, wound and ulcer healing and for topical application. More pharmacological and toxicological studies are required to further elucidate the mechanisms of action, potential side effects, and optimal dosages for these treatments. Additionally, more clinical trials are needed to validate these traditional uses in human populations and to ensure the safety and efficacy of V. nilotica for these applications. This article offers an overview of therapeutic applications by utilizing traditional uses and recent findings on phytochemical studies, and clinical and pharmacological research.
Acrylamide Exposure and Cardiovascular Risk: A Systematic Review
Authors: Mérida Diana María , Rey-García Jimena , Moreno-Franco Belén , Guallar-Castillón Pilar
Published: December 2024

Background/Objectives: Acrylamide is a food contaminant formed during high-temperature cooking processes, leading to unintentional human exposure. Diet is the primary source for non-smokers, with potatoes, cereals, and coffee being the main contributors. While animal studies have demonstrated that acrylamide is neurotoxic, genotoxic, mutagenic, and cardiotoxic, its effects on human cardiovascular health remain poorly understood. This study aimed to evaluate the association between acrylamide exposure and cardiovascular risk. Methods: A comprehensive literature search was conducted across four databases without restrictions on publication year or language (last search: 1 July 2024). The risk of bias was assessed using the Joanna Briggs Institute critical appraisal tools. Results: In total, 28 studies were included, predominantly from the US NHANES sample and with cross-sectional designs. Higher acrylamide exposure was associated with an increased risk of cardiovascular mortality but was inversely associated with glucose and lipid levels, as well as key cardiovascular risk factors such as diabetes, obesity, and metabolic syndrome. Conversely, glycidamide—acrylamide’s most reactive metabolite—was positively associated with elevated glucose and lipid levels, higher systolic blood pressure, and increased obesity prevalence. Conclusions: These findings suggest that the adverse cardiovascular effects of acrylamide may be mediated by its conversion to glycidamide. Further research is necessary to fully elucidate the impact of acrylamide on cardiovascular health. Meanwhile, public health efforts should continue to focus on mitigation strategies within the food industry and raising public awareness about exposure.
Are Foods with Protein Claims Healthy? A Study of the Spanish Market
Authors: Beltrá Marta , Borrás Fernando , Ropero Ana B.
Published: December 2024

Background: Foods with protein claims (PCs) targeted at the general population are increasingly sought after by consumers because they think they are healthy. However, they may contain other nutrients that pose a health risk. Objectives: Therefore, the aim of this work was to carry out a comprehensive evaluation of foods with PC and compare them with those without these claims. Methods: The Spanish Food Database, BADALI, was used for this purpose. We studied 4325 processed foods of 12 different types. Thirteen percent had PCs and more than half of them were fortified with proteins (60.4%). Plant proteins were added more frequently than animal proteins. Protein values were higher in foods with PCs, particularly in those that were fortified. Differences in other nutrients were also observed depending on the food type. The healthiness of foods was assessed using the Pan American Health Organization Nutrient Profile Model and 90.8% of those bearing PCs were classified as “less healthy”. More than 50% were high in fat or high in sodium; around one in four were high in free sugar or saturated fat and one in five had sweeteners. Foods with PCs had 13.1% more “less healthy” items than those without PCs. The proportion of items high in fat or high in sodium were also larger and more of them had sweeteners. In contrast, the proportion of foods high in free sugar and high in saturated fat was lower among those with PCs. Conclusions: Therefore, the perception that foods with PCs are healthy is incorrect, and consuming them may pose an additional health risk.
Effectiveness of Dietitian-Involved Lifestyle Interventions in Diabetes Management Among Arab Populations: A Systematic Review and Meta-Analysis
Authors: Sheffer-Hilel Galia , Abd Elqader Omaima , Suliman Layla , Srulovici Einav
Published: December 2024

Background/Objectives: Diabetes prevalence is high among Arab populations, where cultural practices present barriers to effective glycemic control. Despite guidelines recommending the involvement of dietitians in diabetes management, evidence of the effectiveness of dietitian-involved interventions in these populations remains limited. This systematic review and meta-analysis evaluated the effectiveness of dietitian-involved lifestyle interventions among Arab populations with prediabetes or diabetes. Methods: PubMed, CINAHL, and the Cochrane Library were systematically searched for studies employing experimental and quasi-experimental designs with interventions involving dietitians. All reported outcomes were considered to ensure a comprehensive review. The protocol was registered in PROSPERO (registration number CRD42024555668). Results: The meta-analysis showed significant reductions in glycosylated hemoglobin (HbA1c) levels (−0.41; 95% CI: −0.67, −0.16), body mass index (BMI) (−0.28; 95% CI: −0.36, −0.19), and increases in high-density lipoprotein cholesterol HDL-C) (0.60; 95% CI: 0.36, 0.85) compared to usual care. Subgroup analysis indicated variations based on follow-up duration. The certainty of the evidence was low due to heterogeneity and potential biases, emphasizing the need for further high-quality research to confirm these findings. Conclusions: Interventions involving dietitians improve glycemic control and metabolic outcomes among Arab populations. Given the unique cultural challenges in this population, culturally tailored and personalized interventions are essential to overcome barriers and improve diabetes-related outcomes. Future studies should focus on expanding the evidence base, integrating these findings into healthcare policies, and exploring strategies for long-term sustainability and broader application.
Development of a PET Probe Targeting Bromodomain and Extra-Terminal Proteins for In Vitro and In Vivo Visualization
Authors: Wang Yongle , Wang Yanli , Xu Yulong , Cheng Hua , Dagnew Tewodros Mulugeta , Kang Leyi , Tocci Darcy , Shen Iris Z. , Zhang Can , Wang Changning
Published: December 2024

Background: Bromodomain and extra-terminal (BET) proteins are critical regulators of gene transcription, as they recognize acetylated lysine residues. The BD1 bromodomain of BRD4, a member of the BET family, has emerged as a promising therapeutic target for various diseases. This study aimed to develop and evaluate a novel C-11 labeled PET radiotracer, [11C]YL10, for imaging the BD1 bromodomain of BRD4 in vivo. Methods: [11C]YL10 was synthesized and evaluated for its ability to bind to the BD1 bromodomain selectively. PET imaging studies were conducted in mice to assess brain penetration, pharmacokinetics, and selectivity. In vitro autoradiography and blocking experiments were performed to confirm the tracer’s specificity for the BD1 domain. Results: [11C]YL10 demonstrated good brain penetration, high selectivity for the BD1 bromodomain, and favorable pharmacokinetics in initial PET imaging studies. In vitro autoradiography and blocking experiments confirmed the specific binding of [11C]YL10 to the BD1 domain of BRD4, further validating its potential as a targeted radiotracer. Conclusions: The development of [11C]YL10 provides a new tool for studying BRD4 bromodomains using PET imaging technology. This radiotracer offers potential advancement in the diagnosis and research of neurodegenerative diseases and related disorders involving BRD4 dysregulation.
Seasonal Morphological and Biochemical Variation of Coffea canephora Pierre ex A. Froehner (Rubiaceae) Leaves of Early, Intermediate and Late Maturing Genotypes
Authors: Crasque Jeane , Lira Jean Marcel Sousa , Polonini Giuseppe Tognere , Souza Thiago Corrêa de , Schmildt Edilson Romais , Arantes Lúcio de Oliveira , Dousseau-Arantes Sara
Published: December 2024

Understanding the growth patterns of genotypes optimizes their selection and management. The objective of this study is to investigate the seasonal variations in the morphology and biochemistry of Coffea canephora clone leaves, considering climatic conditions and the maturation cycle. Morphological characteristics and carbohydrate contents of the leaves were analyzed throughout the growth cycle. A nonlinear logistic model was applied, and critical points of the leaf emission rates of plagiotropic branches were determined. Leaf growth was greater at higher temperatures during the rainy periods and lower at milder temperatures during the dry season. Genotype 143 exhibited the largest leaf width in spring, while 104, A1, and P2 had the largest leaf width in summer. The logistic model was suitable for describing leaf emission, with the critical points of genotype 143 being earlier, while P2 displayed a longer leaf emission cycle. The peak growth period influenced the quantities of starch and total soluble sugars in the leaves. The dormancy period showed a higher availability of reducing sugars. Pearson correlation indicated significant coefficients between temperature, precipitation, photoperiod, and foliar characteristics. The results obtained serve as a reference for future investigations, particularly in response to environmental challenges.
Nanopore Data-Driven Chromosome-Level Assembly of Flax Genome
Authors: Arkhipov Alexander A. , Pushkova Elena N. , Bolsheva Nadezhda L. , Rozhmina Tatiana A. , Borkhert Elena V. , Zhernova Daiana A. , Rybakova Tatiana Yu. , Barsukov Nikolai M. , Moskalenko Olesya D. , Sigova Elizaveta A. , Dvorianinova Ekaterina M. , Melnikova Nataliya V. , Dmitriev Alexey A.
Published: December 2024

Flax is an important crop grown for seed and fiber. Flax chromosome number is 2n = 30, and its genome size is about 450–480 Mb. To date, the genomes of several flax varieties have been sequenced and assembled. However, the obtained assemblies are still far from the telomere-to-telomere (T2T) level. We sequenced the genome of flax variety K-3018 on the Oxford Nanopore Technologies (ONT) platform and obtained 57.7 Gb of R10 simplex reads with an N50 = 18.4 kb (~120× genome coverage). ONT reads longer than 50 kb were kept as ultra-long ones (~10× genome coverage), and the rest of the ONT reads were corrected using the HERRO R10 model (quality > Q10, length > 10 kb, ~60× genome coverage remained). The genome was assembled using Hifiasm and Verkko. The Hifiasm-generated assembly was 489.1 Mb in length with 54 contigs and an N50 = 28.1 Mb. Verkko produced a very similar but more fragmented genome: 489.1 Mb, 134 contigs, N50 = 17.4 Mb. In the assembly by Hifiasm, eight chromosomes consisted of a single contig with telomeric repeats at both ends. In addition, five chromosomes comprised two contigs and two chromosomes comprised three contigs. These chromosomes also had telomeric repeats at their ends. The Hifiasm-generated assembly of variety K-3018 had similar contiguity but was likely more complete and accurate than the main fifteen-chromosome assembly of variety YY5 (produced from PacBio data and scaffolded with Hi-C data), the most contiguous flax genome assembly at the time of this writing. We suggest that sufficient genome coverage with long ONT R10 simplex reads is a viable alternative to PacBio plus Hi-C data for a high-precision T2T genome assembly of flax, opening new perspectives for whole-genome studies of flax.
Harnessing Single-Cell and Spatial Transcriptomics for Crop Improvement
Authors: Hu Yuzhao , Dash Linkan , May Gregory , Sardesai Nagesh , Deschamps Stéphane
Published: December 2024

Single-cell and spatial transcriptomics technologies have significantly advanced our understanding of the molecular mechanisms underlying crop biology. This review presents an update on the application of these technologies in crop improvement. The heterogeneity of different cell populations within a tissue plays a crucial role in the coordinated response of an organism to its environment. Single-cell transcriptomics enables the dissection of this heterogeneity, offering insights into the cell-specific transcriptomic responses of plants to various environmental stimuli. Spatial transcriptomics technologies complement single-cell approaches by preserving the spatial context of gene expression profiles, allowing for the in situ localization of transcripts. Together, single-cell and spatial transcriptomics facilitate the discovery of novel genes and gene regulatory networks that can be targeted for genetic manipulation and breeding strategies aimed at enhancing crop yield, quality, and resilience. This review highlights significant findings from recent studies, discusses the expanding roles of these technologies, and explores future opportunities for their application in crop improvement.
Preparation and Characterization of a Novel Self-Healing Transparent Polyimide Film Based on Dynamic Disulfide Bonds
Authors: Li Xin , Zhai Yan , Yang Kai , Bai Jingjing , Qiu Yu , Wang Yulong
Published: December 2024

Self-healing optically transparent polyimides have potential applications in optoelectronic device fabrication. In this study, for the first time, we successfully prepared a novel self-healing polyimide film containing reversible disulfide bonds through chemical imidization by introducing cystamine as a self-healing functional monomer into the molecular structure of conventional polyimides. The incorporation of cystamine enabled the films to maintain high transmittance (>87%) and tensile strength (>99 MPa). Meanwhile, tensile tests showed that the prepared film with a cystamine content of 50% achieved an excellent self-healing efficiency of up to 91.8%. Stress relaxation tests further revealed that disulfide bonds were rapidly cleaved upon thermal stimulation and the network topology was rearranged to complete the self-healing process. These results suggest that the dynamic covalent polymer network made of aliphatic disulfide bonds presents a new strategy for the development of optically transparent polyimides with excellent self-healing properties.