bluuebunny/crossref_metadata_2025 huggingface dataset:
A CONCEPTUAL MODEL OF ETHICAL SERVICE COMPLIANCE AND CULTURE FOR BANKING SECTOR
Authors: Rachmat Basuki , Surachman Surachman , Thoyib Armanu , Rohman Fatchur
Published: November 2020

The objective of this study is to propose a conceptual model of ethical service compliance and culture for the banking sector in order to comply with ethical service and local wisdom which has been argued as one of the specific domains. The review of the literature was conducted to examine the existing service culture model and theoretical concept. PICO (Problem, Intervention, Comparison, and Outcome) technique was utilised to identify the service domains which suit the banking sector. This study found that service culture needs to include the service domains since the early stage of the recruitment process and training. It is also important to consider the service reward, service co-creation, service transformation, service climate dan service orientation as the drivers of ethical service compliance and culture, which drives toward an excellent service value.
Key accents of agenda of industrial development in Ukraine
Authors:   Institute of Industrial Economics of the NAS of Ukraine, Zbarazska Larysa
Published: November 2020

Industry of Ukraine requires cardinal changes both for overcoming of the current crisis phenomena and for providing of favourable conditions for dynamic and effective increase in a long-term prospect. Actuality of problem grows under impact of scale transformations in geopolitical and geoeconomical landscapes. The key trends of industrial development in the world are determined by the processes of introduction of innovative technologies of fourth industrial revolution (4IR). However, at present starting positions of national economy and industry in particular are unfavorable for realization of such projects. The problem of disparity of necessity and possibilities transition of industrial production to the new technological level must be settled by continuous adjustment of industry development strategy and state tactics to the specific national aims and conditions (macroeconomic, institutional, foreign-policy) The aim of this article is to accent attention on actual directions, mechanisms, and instruments, which are most accessible, acceptable (economical and institutional measuring) and effective for realization of industrial development strategy under existing conditions in Ukraine. In the article the "road map" of becoming modern industrial production in Ukraine is offered. It covers five purposeful directions (blocks): science and innovation, finance and investment, institutional, structural changes, and market demand. Within each of them tendencies are analysed and priorities for public industrial policy are formulated. The significance and arguments for the urgent formation of national scientific and innovative potential as a factor in reducing the dependence of production and reproduction processes in industry on external influences are presented. The institutional block covers the issue of uncertainty of strategic prospects of industrial development in Ukraine as a deterrent factor to the necessary transformations. The importance of realization of the strategic role of the state at the present stage of industrial transformations is argued. The objective need to involve big business in the intensification of innovation processes based on various forms of partnership with the state is revealed. Emphasis is placed on the priority tasks of structural and sectoral development in the context of accelerating the implementation of modern technological solutions and product innovations. In particular, the importance of the manufacturing sectors of defence and ICT is accented. The importance of expansion and diversification of domestic and foreign markets for the development of high-tech sectors of in national industry is considered.
Conceptual issues of innovation networks digitalization
Authors:   Institute of Industrial Economics of National Academy of Sciences of Ukraine, Shevtsova Hanna , Omelyanenko Vitaliy , Prokopenko Olha ,   Institute of Industrial Economics of National Academy of Sciences of Ukraine,   Tallinn University of Technology Estonia
Published: November 2020

The relevance of the research topic is due to the need to solve the problem of developing conceptual issues of digital transformation management in the context of innovation trends, taking into account the overarching impact and large-scale prospects of digitalization processes. The paper deals with the analysis of the digital aspects of innovation policy, in particular, based on the use of international rankings, the definition of features of building the digital component of innovation networks and the deepening of scientific and methodological approaches to digital support of innovation processes. An overview of modern scientific and methodological approaches to defining the impact of digital transformation on innovation process is presented. Digital transformation can be seen as a factor in access to information and new opportunities that can improve technological capabilities. To assess the impact of digitalization on the development of innovation processes, the data of the Global Innovation Index and the ICT Development Index were used. Based on results of calculations, significant values of the positive correlation with the key components of the innovation system and the level of cluster development were obtained. The study identifies marketing, communication, infrastructural, international dimensions of digitalization of innovation processes. It is shown that the increasing availability of ICTs necessitates a timely rethinking of many processes in the innovation sphere. On the basis of world experience, the role of universities has been substantiated, which should take on a significant part of the responsibility for the formation of a new technological order through improving the quality of researches and their results’ introduction into the real economy within the framework of S2B-B2S networks (Scіence-to-Busіness – Busіness-to-Scіence). As a result of the study, the conclusion is substantiated that an integrated approach to the creation of digital innovation ecosystems requires the study of both institutions and participants, their interaction networks, the specifics of the environment, resources, technologies, etc. It is proposed to consider the role of ICTs in the development of innovation networks on the basis of combining such modern approaches to the development of innovations, in particular – multidisciplinary engineering analysis, system design methodology and parallel engineering methodology. The analysis and deepening of scientific and methodological approaches to the digital support of innovation processes made it possible to identify other promising areas of digitalization of innovation processes, for example, within the framework of the activities of public authorities that ensure the introduction of electronic government technologies.
Comparative Analysis of Indonesian Presidents’ Identities in Wayang during the Reformasi Era
Authors: Elias Stanley
Published: November 2020

This study comparatively analyses the allusion and analogies of Indonesian Presidents’ by wayang characters and stories. It particularly interrogates how wayang characters and lakon allude and mediate the personal and political identities of Indonesian presidents in the Reformasi era. The study focuses on the portrayal of President Susilo Bambang Yudhoyono and Jokowi during Reformasi era. The study is a qualitative research and deploying Textual and Critical Discourse Analysis approaches. These are to examine how language in the selected wayang kulit and wayang golek stories, as a form of social and cultural practice, constructs the personal and political identities of the presidents. Central to the analysis, Susilo Bambang Yudhoyono is in most cases alluded as Semar, and his association with selected lakon significantly inform on the political and personal identities among Indonesians and the International community. On the other hand, Jokowi is alluded to as Petruk but presenting contradicting allusions and analogies in Indonesian politics. Generally, findings suggest that the portrayal of Indonesian presidents’ identities by wayang characters and in the lakon is never fixed. The change of political atmosphere and failure to meet peoples’ expectations culminate in changes on the portrayal of political and personal identities of presidents.
Socio-Psychological Factors as Determinants to Information-Seeking Behavior of LIS Undergraduates in Kwara State
Authors: Sulaiman Kabir Alabi
Published: November 2020

This study examines socio-psychological factors as determinants to information-seeking behaviour of LIS undergraduates in Kwara state. A descriptive survey method was adopted. A questionnaire was used to collect data for the study. The total population for the study is 2392. From which the sample size of 333 was selected using Raosoft size calculator. The study revealed that most of LIS undergraduate have fear during information searching process. The study also revealed that LIS undergraduates are of opinions that having access to the internet improves their ability to search for information. It was also found out that most of LIS undergraduates were helped with internet to have access to various sources of information simultaneously. In conclusion, there is a significant relationship between computer/internet and information-seeking behaviour of LIS undergraduates in Kwara state. This research recommends that LIS undergraduate should improve confidence in the use of internet/computer to seek information with ease and LIS undergraduate should develop a mechanism for self-confidence while seeking for information
Ideology, Humanity, and Freedom in Ha Jin’s Waiting
Authors: Djohar Hasnul Insani
Published: November 2020

This paper examines how Ha Jin’s Waiting challenges the Maoist communist regime by depicting the protagonist, Lin, as struggling to fight for his rights to live freely. The Maoist regime successfully establishes “normalizing power” in a society to lead the protagonist to believe that the goal of his life is mainly for working hard for the military institution and the regime, instead of establishing his freedom. As a result, Lin loses his senses of humans, such as love and empathy, and lives with selfishness and ignorance as to the way the Maoist discourse teaches him through Mao’s red book. By engaging with cultural studies, this paper investigates how Jin’s Waiting challenges Maoist ideology by both celebrating and critiquing the idea of capitalism, which likely perpetuates communism. Thus, this paper discovers how Ha Jin’s novel challenges communist ideologies and totalitarian rules by illuminating social disorder and loses of sense of humanity. Indeed, individuals live under oppression and they are like a prisoner who is suffering from being judged and punished by totalitarian regimes and dominant society. Hence, the significance of this research is to help to reduce any forms of oppression experienced by many ethnic-Americans who have suffered from the totalitarian rulers that have ruled society, especially in the era of communism, colonialism, and global capital transnationalism.
Epidemiology and antibiotic resistance profile of bacterial meningitis in Morocco from 2015 to 2018
Authors: Ikken Youssef , Charof Réda , Benaouda Amina , Hilali Farida , Akkaoui Sanae , Elouennass Mostafa , Sekhsokh Yassine
Published: November 2020

Over a 4-year study period from 2015 to 2018, altogether 183 isolates of bacterial meningitis were collected from 12 hospitals covering the entire Moroccan territory. Neisseria meningitidis represented 58.5%, Streptococcus pneumoniae 35.5%, and Haemophilus influenzae type b 6%. H. influenzae type b mainly affected 5-year-olds and unvaccinated adults. N. meningitidis serogroup B represented 90.7% followed by serogroup W135 with 6.5%. Decreased susceptibility to penicillin G (DSPG) for all isolates accounted for 15.7%, with 11.6% being resistant to penicillin G (PG) and 4.1% decreased susceptibility. Cumulative results of all strains showed 2.7% decreased susceptibility to amoxicillin and 3.3% resistant, 2.2% of isolates were resistant to third-generation cephalosporin and 2.2% were decreased susceptible, 5.5% were resistant to chloramphenicol and 2.7% were resistant to rifampin. The frequency of DSPG observed in our study is more common in S. pneumoniae than in N. meningitidis (P < 0.05). These isolates have been found to be highly susceptible to antibiotics used for treatment and prophylaxis chemotherapy and the observed resistance remains rare. The impact of introduction of conjugate vaccines against H. influenzae type b and S. pneumoniae (PCVs) is an advantage in reducing meningitis cases due to these two species.
Mentoring valorization in combating violent and aggressive behaviour in preadolescence and adolescence
Authors: Diana Antoci , Valentina Mislitchi , Maria Diacon
Published: November 2020

The present work combats the problem of violence and aggressive behaviour through mentoring. The theoretical point of view certified studies that highlight various aspects of the problem of violent and aggressive behaviour remediation of preadolescents and adolescents. At present there are no publications that completely synthesize the institutional and normative field of mentoring. The existing publications reference to the regulation regarding the establishment of the mentoring system for teaching staff in education. Thus, the problem of mentoring valorization in combating violent behaviour among preadolescents and adolescents remains unresolved. The viable solution to solving these actual problems of the postmodern world is to focus on the personality value system.
Eating disorder characteristics among Hungarian medical students: Changes between 1989 and 2011
Authors: Túry Ferenc , Szabó Pál , Dukay-Szabó Szilvia , Szumska Irena , Simon Dávid , Rathner Günther
Published: November 2020

Background and aims

There are contradictory findings on time changes in the prevalence of eating disorders (EDs). The first epidemiological studies in Hungary were carried out in the late 1980s. The objective of the present study was to follow the changes in the prevalence of EDs in medical students after a period of 22 years.

Methods

A questionnaire survey was conducted in 1989 and in 2010. The sample comprised medical students: 538 subjects (248 males and 290 females) in 1989 and 969 subjects (261 males and 708 females) in 2010. The questionnaire contained sociodemographic and anthropometric items, the Eating Behaviour Severity Scale, the General Health Questionnaire, the Anorexia Nervosa Inventory for Self-Rating, and the Eating Disorder Inventory (EDI). In the second wave, three subscales of the EDI-2 and the SCOFF questionnaire were added.

Results

Current and desired body mass index were significantly higher in the second study. Binge eating at least once a week was reported less frequently (2.7% vs 6.8% in males, 6.1% vs 13% in females) in 2010. The proportion of subclinical anorexia nervosa was higher among females in 2011 (2.5% vs 0.3%,P< 0.01). Among males, the proportion of counterregulatory behaviours increased significantly (from 8.9 to 14.6%).

Discussion and conclusions

The increase of the proportion of subclinical anorexia nervosa and that of male EDs may relate to the importance of the changes in the sociocultural background. Further representative studies are proposed in other countries of Central and Eastern Europe among medical students and in the general population.
Personal choice is a nuanced concept – Lessons learned from the gambling field. •
Authors: Hodgins David C.
Published: November 2020

This thoughtful framework to minimize the harm associated with emerging technologies by encouraging collaborations among stakeholders would benefit from adopting the WHO precautionary principle in order to keep public health issues at the core of discussions. It would also be helpful to acknowledge and make transparent the differences in stakeholder priorities, the power differentials among stakeholders, and the importance of institutional duty of care.
UJI HEDONIK: PENGKAYAAN NUTRISI DARI COOKIES COKLAT TEPUNG MANGROVE (Avicennia officinalis) DENGAN PENAMBAHAN TEPUNG KACANG MERAH, TEPUNG WIJEN, DAN TEPUNG HATI AYAM
Authors: Harahap Kurnia Sada , Sumartini Sumartini , Mujiyanti Apri
Published: November 2020

Cookies adalah makanan ringan yang sering dikonsumsi oleh masyarakat karena rasanya yang manis dan teksturnya yang crunchy. Penambahan variasi tepung kacang merah, tepung wijen, dan tepung hati ayam  dapat meningkatkan kandungan zat besi dan kalsium pada Cookies. Selain itu, penambahan tepung buah mangrove api-api  dapat dijadikan alternatif pengganti tepung terigu, karena mengandung kadar karbohidrat, vitamin, dan mineral yang cukup tinggi.  Walaupun subtitusi berbagai jenis tepung tersebut memiliki manfaat dalam konten zat gizi, namun masih terdapat beberapa kendala seperti karakteristik tepung mangrove yang kasar dan aroma yang kurang disukai dari tepung hati ayam membuat Cookies coklat kurang disukai panelis. Oleh sebab itu, penelitian ini bertujuan mencari komposisi terbaik dari tepung mangrove  komposisi dari tepung sub tersebut Tujuan penelitian ini adalah untuk mengetahui tingkat kesukaan panelis dalam pembuatan Cookies coklat tepung buah mangrove api-api dan pengaruhnya terhadap tingkat penerimaan panelis dengan pengujian hedonik. Penelitian ini menggunakan metode Rancangan Acak Lengkap yang terdiri dari 4  formulasi perlakuan yaitu perlakuan A ( 40% Tepung Mangrove, 20% Tepung Mocaf, 20 % Tepung Kacang merah,10%Tepung Hati ayam dan 10% Tepung wijen ) ,B( 50% Tepung Mangrove, 20%Tepung Mocaf, 10 %  Tepung Kacang merah, 15% Tepung Hati ayam dan 5 % Tepung wijen ) ,  C ( 60% Tepung Mangrove,  20% Tepung Mocaf, 5 % Tepung Kacang merah, 10%  Tepung  Hati ayam dan 5% Tepung wijen ) dan D( 100 % Tepung Terigu) sebagai kontrol. Data yang diperoleh dari hasil penelitian akan diuji dengan uji normalitas, uji homogenitas, dilanjutkan dengan Analysis of Varians (ANOVA) dan uji lanjut Beda Nyata Jujur dan pengujian bersih. Sedangkan perlakuan uji panelis  terbaik yaitu formula  dilihat dari tekstur , rasa, warna, dan aroma.
Investigation of antagonistic activities of actinomycetes isolated from Cuc Phuong and Ba Be National Parks against four plant pathogenic fungi
Authors: Thi Van Nguyen , Thi Ngoc Mai Dinh , Thi Hoang Yen Le , Hong Minh Nguyen , Kim Nu Thao Nguyen
Published: November 2020

Fungal diseases are a huge problem in agriculture, causing serious damage to annual crop yields worldwide. In order to control fungal pathogens in plants, using antagonistic microorganisms is a common approach, especially actinomycetes, as biocontrols considered to be very potential and safe for the environment. With the advantage of high levels of biodiversity, Vietnamese actinomycetes are of a valuable resource for finding strains that are capable of biocontrolling fungal pathogens in plants. Thus, in this study, 70 actinomycete strains were isolated from Cuc Phuong and Ba Be National Parks by four different isolation methods including rehydration centrifugation, sodium dodecyl sulfate – yeast extract, dry heating and dilution methods. Of these, 29 strains (41.4%) belong to rare actinomycete genera including Actinoplanes, Acrocarpospora, Dactylosporangium, Kineosporia, Micromonospora, Nocardia, Nocardiopsis and Pseudonocardia, which are rarely studied in Vietnam. Subsequently, 70 actinomycete strains were subjected to antagonistic activity assays against four highly pathogenic fungi in plants including Alternaria sp., Botrytis cinerea, Colletotrichum gloeosporioides and Phytophthora capsici. The results showed that 80.5% of Streptomyces and 31% of rare actinomyces could inhibit the growth of at least one of the four pathogenic fungal strains. Moreover, ten actinmomycete strains were found to be able to inhibit three types of tested fungal pathogens, simutaneously. Especially, four actinomycete strains (VTCC-A-828, VTCC-A-671, VTCC-A-605, and VTCC-A-69), having the highest antagonistic activity against four fungal pathogens Alternaria sp., Botrytis cinerea, Colletotrichum gloeosporioides and Phytophthora capsici, respectively, were selected. The results in this study indicate the great potential application for Vietnam's microorganisms in the field of plant protection.
Mutations in NPHS1 and PLCE1 (NPHS3) in two Vietnamese patients with conginetal nephrotic syndrome
Authors: Lien Nguyen Thi Kim , Van Dem Pham , Thu Huong Nguyen , Minh Dien Tran , Huy Hoang Nguyen , Thi Quynh Huong Nguyen
Published: November 2020

Congenital nephrotic syndrome (CNS), a genetic disease caused by the mutations in genes on autosomes,is usually occurs in the first three months after birth. The mutations in genes that encode for the structural andfunctional proteins of podocytes lead to loss of the function of glomerular filtration. So far, a number of genesrelated to the disease have been identified such as: NPHS1, NPHS2, PLCE1 (NPHS3), ACTN4, CD2AP, INF2and WT1. In this article, we amplified all of exons in NPHS1 and PLCE1 genes of two Vietnamese patientswith CNS and members of patients’ family. PCR products were purified and sequenced directly on automaticsequencer ABI 3500 Bio System (USA). The sequencing results were compared with the sequences of NPHS1and PLCE1 genes published in the Ensembl database (ENSG00000161270 and ENSG00000138193,respectively) by using BioEdit software to detect mutations. We identified two mutations: c.2398C>T(p.Arg800Cys, exon 18), c.3315A>G (p.Ser1105Ser, exon 26) in NPHS1 gene and two mutations: c.5330 C>T(p.Thr1777Ile, exon 23), c.5780A>G (p.His1927Arg, exon 25) in PLCE1 gene in study patients. These twopatients carried simultaneously the mutations in the NPHS1 and PLCE1 genes with serious phenotype. Theresults of our study might be evidences for the role of mutations in NPHS1 and PLCE1 genes in thedevelopment of disease in patients. These are useful information in identifying the cause of disease and providethe genetic counseling to the patients’ family.
Evaluating the qualities of probiotic products by a DGGE-based procedure
Authors: Hanh Tran My , Thi Dung Cao , Thi Thanh Huyen Tran , The Hai Pham
Published: November 2020

The quality of the probiotics is determined by the microbiological composition and can thus be verified by analyzing this characteristic. The use of the Denaturing Gradient Gel Electrophoresis (DGGE) allows the detection of each microorganism through the corresponding band on the gel, which can be considered as the "bar code" of that microorganism. This "bar code” principle demonstrates the potential for the application of DGGE technology to test microbial probiotics with or without a bacterium as registered to assess whether they are qualified. As such, in this study, we experiment the application of DGGE method to test 10 commercial probiotic products, with a focus on products consisting of only bacteria which dominate the market. First, we performed DNA genome extraction and amplification of 550 bp DNA of the 16S rDNA by PCR. Then, the  amplicon was analysed on polyacrylamide gels containing a 30% to 60% linear denaturing gradient of urea and formamide. Microbial composition of 7 of the 10 products showed a strong correlation between DGGE results with product labeling as well as isolation of micoorganism. This study thus demonstrates the potential application of DGGE technology in evaluating the qualities of probiotic products.
Whole exome sequencing identified a novel myopalladin gene mutation in a cardiomyopathy patient
Authors: Chi Bao Bui , Minh Hiep Nguyen , Manh Cong Nguyen , Thi Thu Trang Pham , Thi Tham Luong , Thanh Nga Ha Thi , Bao Quoc Vu , Thuat Khoa Pham Ho , Huynh Nga Nguyen Thi
Published: November 2020

Cardiomyopathies (CMs) are a heterogenous group of disorders that affects the heart muscle. Incardiomyopathies, phenotypic overlapping among the inherited cardiovascular diseases (CVDs) limits theability to establish a diagnosis based solely on clinical features. Here, we developed a next generationsequencing (NGS) assay to analyze a panel of 142 known cardiomyopathy genes in 9 Vietnamese patientsfrom Children Hospital 2, Hochiminh City and Medical University Hospital, Hochiminh City, Vietnam.Whole exome sequencing (WES) - a technique which determines the variations of all coding regions (exons)of the known genes - validated a total of 65 rare variants in 18 cardiomyopathy genes among the studiedVietnamese unrelated patients. Of 65 variants identified, 28 variants were homozygous and the other 37 oneswere heterozygous. Among the 65 variants, TTN gene variants accounted the most for 13 mutations, which areknown to be benign. Other groups of 9 and 8 mutations belong to SYNE1 and MYPN genes, respectively. Tenout of 65 mutations distributed equally to NDUFV2 and SCN5A gene variants. We detected 6 and 4 variantsfor SYNE2 and COX15 genes, respectively. Each gene of DMD, KCNE1, NEBL and RBM20 has 2 variants. Asingle variant was detected for AKAP9, CAV3, DSC2, DSG2, DSP, MYBPC3 and MYH6 genes. Especially,among them, we found a novel heterozygous nonsynonymous mutation c.1527C>G on the MYPN gene. Thesegenetic results support the “pan-cardiomyopathy panel” approach, by which the molecular diagnosis ofcardiomyopathies, early identification of arrhythmia development and better clinical management ofcardiomyopathic patients are applied.
Structure and characterization of the ribosomal transcription units of small liver flukes, Opisthorchis viverrini, O. Felineus and Clonorchis sinensis
Authors: Thanh Hoa Le , Thi Bich Nga Nguyen , Thi Thanh Huong Doan , Kim Xuyen Le Thi , Thi Khue Nguyen
Published: November 2020

Opisthorchiasis is a zoonotic parasitic infection caused by small liver fluke species, Opisthorchis viverrini,O. felineus and Clonorchis sinensis, in the family Opisthorchiidae. Vietnam has both species, of which C.sinensis is distributed in the northern and O. viverrini in the central provinces. In addition to the mitochondrialgenomes, the ribosomal DNA sequences (rDNA) of these species are highly needed to obtain for providingmolecular markers in species identification, classification, phylogeny and evolutionary studies. In this study,the near/complete nucleotide sequences of ribosomal transcription units (rTU) from O. viverrini (Vietnamesesample), O. felineus (Russian sample) and C. sinensis (Vietnamese sample) were analyzed. All rTUs for threespecies were determined, which is 7,839 bp for O. viverrini, 6,948 bp for O. felineus and 7,296 bp for C.sinensis containing structures of 18S, ITS1, 5,8S, ITS2 and 28S. The IGS region was not obtained for all threespecies. In all three species, sequence analysis revealed 2 tandem repetitive elements of 47-48 bp/each in ITS1but not in ITS2. The nucleotide sequences of 18S, ITS1, ITS2 and 28S are valuable ribosomal markers that thisstudy provides for diagnosis, identification, taxonomic classification and population genetics. In conclusion,the rTU sequences for the three species of the family Opisthorchiidae have been identified and providesmolecular markers for the use of phylogenetic analysis for species/family classification in the superfamilyOpisthorchioidea and the class Trematoda.
Structure of insulin-like growth factor 2 (IGF2) gene from striped catfish (Pangasianodon hypophthalmus)
Authors: Thi Nguyen Binh Le , Thi Hoa Nguyen , Trang Tran Thi Huyen , Phuong Nguyen Thanh , Oanh Kim Thi Phuong
Published: November 2020

Gene coding Insulin-like growth factor 2 (IGF2) is one of genes relating the growth of many fish species, including striped catfish. In this study, cDNA coding to protein IGF2 was isolated from total RNA extracted from liver tissue of catfish, then was amplified through reverse transcription PCR and standard PCR with IGF2 specific primer pair to generate the 642bp-in-size PCR product. The sequence of this cDNA-IGF2 is similar up to 95% to the cDNA-IGF2 gene sequence of catfish included in family Ictalurus. Based on that sequence, 3 primer pairs were designed to amplify 3 regions of IGF2 gene from genomic DNA extracted from fins of catfish, which have 900bp, 1500bp and 1200bp in length. That 3 regions were sequenced and assembled to give the final sequence with 3387bp in length of IGF2 gene, which includes 4 exons coding to IGF2 protein, and is similar up to 96% to sequence of mRNA coding to IGF2 protein of channel catfish Ictalurus punctatus and that of blue catfish Ictalurus furcatus. Determining the structure of IGF2 gene of striped catfish Pangasianodon hypophthalmus enable us to further examine its function and genetic variation.
Database construction for Vietnamese catfish genome
Authors: Vu Nguyen Hoang , Phuong Nguyen Thanh , Binh Le Thi Nguyen , Oanh Kim Thi Phuong
Published: November 2020

Molecular biological research plays an important role in aquaculture, contributes to the improvement ofbroodstocks efficiently. Recently, with the development of next-generation sequencing (NGS) technology,genomic studies have been rapidly increased, in which data organisation and management hold a crucialposition. After obtaining NGS sequencing data of Vietnamese catfish (Pangasianodon hypophthalmus), wehave analysed and annotated the catfish genome, from which we have constructed a database for efficientusage. The database is built upon open source software following a three-layer model (interface, Web serviceand database) with a convenient interface through Web browsers. Users can look up sequence and annotationdata as well as visualize sequences through the Jbrowse genome browser. This database is important resourcefor functional genome and genetic improvement of the catfish.
A contribution to the establishment of extract standards from the leaves of Clinacanthus nutans (Burm.f.) Lindau
Authors: Linh Le Nguyen Tu , Quang Dao Vu , Dinh Thach Bui , Thi Ben Trinh , Thi Linh Giang Tran , Kim Thach Le
Published: November 2020

Nowadays, herbs have become a popular form of healthcare. Clinacanthus nutans (Burm.f.) Lindau (family Acanthaceae) is popular in Asia, especially in the tropical countries. Extracts of C. nutans leaves are widely used to treat diseases of skin, anti-inflammatory, anti-virus, anticancer, antioxidant, antidiabetic, immunomodulatory, wound healing and analgesic activities, etc. This study determined the parameters of ethanol extract of C. nutans leaves including pH, total weight loss due to water, total ash content, heavy metal content, microbiological limits, pesticide residual content, presence and contents of C-flavones. The C-flavones such as shaftoside, orientin, isovitexin and vitexin have been found to be major flavonoids in the leaves of this plant. Therefore, we had used a two-step method using thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC) for the rapid identification and quantification of the flavones C-glycosides in ethanol extract C. nutans leaves. These results provided useful information for the evaluation of quality of C. nutans raw materials and its commercial products. Moreover, that contributed to the establishment of extract standard from the leaves of C. nutans. The parameters of the ethanol extract of C. nutans leaves were obtained as follows: pH (5,23 ± 0,35), total weight loss due to water (15,62 ± 0,25%), total ash (13,85 ± 0,98%), microbiological limits are achieved the requirement of medicinal standards, content heavy metal and residue of pesticide are not detected. Contents of C-flavones: shaftoside (0,69 ± 0,04 mg/g), orientin (0,24 ± 0,008 mg/g), isovitexin (0,015 ± 0,003 mg/g), vitexin were very low or undetectable.
Conjugation of nanomaterials containing rare-earth ion Tb3+ with CD133 monoclonal antibody and its potentials for labeling colon cancer cells (HT-29)
Authors: Nhat Minh Le , Trong Nhan Vo , Thi Nga Nguyen , Thu Huong Tran , Thi Kim Hue Phung , Thi Thao Do
Published: November 2020

Nanotechnology is the key technology that brings many important applications in biomedical research.Nanolantanites present high stability, easy fabrication and functionalization. Tb3+ ion-containing nanomaterial, a specific type of nanolantanites, possess great prospects. In addition, cancer stem cells (CSCs) are directlyrelated to drug resistance, metastasis, recurrent cancer, etc. Therefore, CSCs are considered as the target forcancer researching and for discovery of more effective therapies. CD133, a trans-membrane glycoprotein, isone of the typical markers that are found to appear very commonly on the surface of many types of CSCs. Inthis study, CD133 monoclonal antibody (MAb) was cojugated with nanomaterials containing Tb3+. Thecoupling between fluorescented nanomaterials containing Tb3+ ions and CD133 MAb was then incubated withhuman colon cancer cells (HT-29) to evaluate its ability to label CSCs in vitro. The results showed thatnanorods containing rare-earth based Tb3+ ions which were fabricated by hydrothermal method, present thelength of about 300 - 800 nm and the diameter in range of 40 - 50 nm. The Tb3+ nanoparticals also havehexagonal structure of terbium phosphate monohydrate and green illuminant. Tb3+ nanorods were also furthersurface silica coated and amino-silane functionalized. This nanostructure was successfully combined withmonoclonal antibodies against CD133 which labelled the surface marker of HT-29 human colon cancer cells.As a result, the combination of CD133+TbPO4@Silica-NH2 (functionalized surface) showed strongerluminescence than the CD133+TbPO4 unfunctionalized combination.
Applications of fluorescence in situ hybridization (FISH) in genome research
Authors: Nhu Phuong Hoang Thi , Thu Hue Huynh Thi , Xuan Hieu Cao
Published: November 2020

Fluorescence in situ hybridization (FISH) technique enables the direct detection of DNA sequences inintact cellular materials (e.g. individual chromosomes in metaphase spreads). This review article focuses on theapplications of FISH in genome research, including validation and correction of the genome assembly from thenext-generation sequencing (NGS) projects. DNA probes for specific DNA fragments of the assembly can beobtained from PCR amplicon or cloned products using different vector systems. Localization of these probeson their respective chromosomal regions can be visualized by FISH, providing useful information to crosscheckthe assembly data. Furthermore, the recent refinements in the FISH technology including using smartpooling scheme of differently colored DNA probes, together with consecutive FISH experiments (stripping andreprobing of the same slide) are described. These advances in multicolor FISH can provide crucial linkageinformation on association of linkage groups and assembly scaffolds, resulting in so-called cytogenetic maps.Integration of the cytogenetic maps and assembly sequences assists to resolve the chromosome-level genomeassembly and to reveal new insights in genome architecture and genome evolution. Especially, comparativechromosome painting with pooled DNA probes from one reference species can be used to investigate ancestralrelationships (chromosome homeology and rearrangements) among other not-yet-sequenced species. Inaddition, FISH using DNA probes for certain specific classes of repetitive DNA elements as well as for basicchromosome structures (e.g. centromere or telomere DNA repeats, ribosomal DNA loci) can be used to studythe genome organization and karyotype differentiation. We also discussed about limitations and futureperspectives of the FISH technology.
Interactionsbetween of Orius sp. (Heteroptera: Anthocoridae) and Amblyseius (Typhlodromips) Swirskii (Athias-Henriot) (Acari: Phytoseiidae) for biological control of Thirps palmi karny (Thysanoptera: Thripidae) on muskmelon
Authors: Thi Thuy Nguyen , Van Dai Nguyen , Thi Phuong Thao Nguyen
Published: November 2020

In this study, we examined biological control Thirps palmi Karny of a predatory mite Amblyseius (Typhlodromips) swirskii (AthiasHenriot), the anthocorid bug Orius sp. and combination of Amblyseius swirskii and Orius sp. The experiments in laboratory condition showed that combination of these two species have the ability to prey consumtion is higher than using only predator (Orius sp. or Amblyseius swirskii). The fecundity of Orius sp. in the absence of Amblyseius swirskii (the total number of eggs/ females = 45.5 ± 2.25 eggs, the oviposition period = 16.49 ± 0.5 days) were higher than in the presence of Amblyseius swirskii (the total number of eggs/ females = 35.6 ± 2.33 eggs, the oviposition period = 13.1 ± 0.63 days). Similar with Amblyseius swirskii, the fecundity of Amblyseius swirskii in the absence of Orius sp. (the total number of eggs/females = 35.57 ± 3.62 eggs, the oviposition period = 21.33 ± 0.7 days) were higher than in the presence of Amblyseius swirskii (the total number of eggs/ females = 24.1 ± 1.67 eggs, the oviposition period = 13 ± 1.43 days). In the greenhouse with area of 300m2, the experiments were conducted when used two-predator were average aduts (Orius sp. were 3.47 adult/ leaf, A. swirskii were 6.96 adult/ leaf) were aqual as release of only Orius sp. (average adult = 3.81 adult/ leaf) or only Amblyseius swirskii (average adult = 6.49 adult/ leaf). This study also provides further evidence that intraguild predation does not necessarily have negative effects on biological control.
Evaluation of the effects of agricultural LED lighting on in vitro propagation of Cordyceps militaris (Link.) Fries
Authors: Hong Gam Do , Huong Huynh Duong , Thi Lan Anh Phan , Hoang Duong Nguyen , Thi Kim Hoa Do
Published: November 2020

In this study, the effects of various agricultural LED lights (LED NN), including single red LED (R), single blue LED (B), and four combinations of blue, red, and warm white (W) LED (BR, BRW1, BRW2, BRW3) on the growth and development of C. militaris (Link.) Fries were evaluated in vitro. After 7 days, samples subjected to LED NN showed shorter sporocarp sprouting time and higher sprouting ratio than the control, which was subjected to T5 fluorescent light. After 2 months, LED lights with high red ratio, such as single red LED and LED BR, had suppressing effect on the growth and development of C. militaris (Link.) Fries. On the other hand, combinations of red, blue, and warm white such as LED BRW1, LED BRW2, and LED BRW3 had the positive impact on the growth and development of this fungus. Notably, samples subjected to LED BRW2 reached 5.79 cm in height, fresh biomass of 3.67 g/20 samples. Cordycepin and Adenosine levels were 64.2 and 6.37 mg/100 g fresh mass, respectively. All of studied  indicators were the higher compared to those of the control and other LED lighting schemes. Therefore, it can be conlcuded that LED lighting combination with BRW2 ratio of 1:5:1 and luminous intensity of 45±2 µmol.m-2.s-1 (511,59 Lux) was suitable for the growth and development of C. militaris (Link.) Friesand a potential replacement of fluorescent light for C. militaris (Link.) Friesin vitro propagation.
Increasing the somatic embryogenesis frequency of Panax vietnamensis Ha et Grushv. by disinfection of leaf explant using nano silver and the addition of nano silver in culture medium
Authors: Cuong Do Manh , Tung Hoang Thanh , Dac Khai Hoang , Quoc Luan Vu , Thi Hien Vu , Thi Bich Phuong Truong , Nhut Duong Tan
Published: November 2020

Somatic embryo is a developmental method for mass multiplication of valuable medicinal plants. In this study, leaf explants of Ngoc Linh ginseng were disinfected with nano silver at different concentrations and exposure times to eliminate infectious agents and induce embryogenic callus for the production of somatic embryos. The results show that the lowest contamination rate (20.00%) was observed when leaf explants were treated with 0.5 g/L nano silver for 15 minutes while the highest embryogenic callus induction rate (72.22%) and fresh weight (0.77 g) was determined at 0.2 g/L nano silver for 20 minutes. High frequency of somatic embryogenesis formation and germination were occurred on MS medium supplemented 1.0 mg/L 2,4-D; 0.5 mg/L NAA; 0.2 mg/L Kin and 1.6 mg/L nanosilver. After 8 weeks of culture, the number somatic embryos derived from nano silver treated-leaves was increased 2 times than non-treated explants. Addition of 1.0 mg/L NAA and 1.2 mg/L nano silver was showed the highest shoot and root length, root number, fresh and dry weight of plantlets. This research showed that pre-treatment and supplement of nano silver in culture medium is potentially useful for improving embryogenesis frequency, and plantlet formation of Ngoc Linh ginseng cultured in vitro.
Detection of DNA of Rickettsia and Orientia tsutsugamushi in rodents and ectoparasites in Ha Giang province
Authors: Lan Anh Le Thi , Viet Cuong Vo , Van Toan Trinh , Thi Hong Nhung Ho , Van Anh Le Thi , Thi Thu Thuy Can , Thi Ha Giang Pham , Thi Thanh Nga Bui , Thi Lan Anh Bui , Van Chau Nguyen
Published: November 2020

Rickettsial fever is one of a zoonotic disease which is caused by bacteria genus Rickettsia. The ectoparasites such as ticks, mites, fleas, lice... were demonstrated as the main transmited vectors through host reservoirs are rodents and small animals including mice, squirrels, mink... In this study, the rodents and ectoparasites species were identified. The molecular detection of Rickettsia was also performed. In 2018, 83 rats were trapped in 2 villages Thanh Duc and Phu Linh, Vi Xuyen district, Ha Giang province, in which 48.2% mice were found as house mice Rattus flavipectus, 21.7% was forest mice R. rattus, 12% was R. fulvescens, 8.4% was R. nitidus, the remaining rates were R. bowersi, Mus. pahari, Leopoldamys sabanus, Mus musculus and R. niviventer, accounting for 1.2% - 3.6%. The ectoparasites survey found 5 chigger mite species including Leptotrombidium (Leptotrombidium) deliense, Ascoschoengastia (Laurentella) indica, Garhliepia (Walchia) rustica, Lorilutum oreophilum and Shunsenia sp as well as 3 gamasid mite species such as Laelaps (Echidninus) sedlaceki, Laelaps (Laelaps) nuttali and Lenstivalius klossi bispiniformis. The result indicated that 19.3% and 10.8% mice were positive with Ricketsia spotted fever group (SFG) and Rickettsia typhi, respectively by real-time PCR. The nested PCR result showed that 19.4% R. flavipectus mice and 10% L. (L.) deliense chigger mites were positive with Orientia tsutsugamushi.
Protection of chickens against A/H5N1 virus by plant-based haemagglutinin fused IGMFC
Authors: Van Pham Thi , Huong Ho Thi , Thu Giang Nguyen , Ngoc Pham Bich , Trang Vu Huyen , Hoang Phan Trong , Hanh Tran Xuan , Conrad Udo , Ha Chu Hoang
Published: November 2020

Vaccination is one of the most effective and cost-beneficial interventions for protection of animals against the highly pathogenic A/H5N1 avian influenza virus. Haemagglutinin (HA) is a transmembrane glycoprotein of A/H5N1 virus and is a critical antigen for development of the influenza vaccine. The haemagglutinin-based vaccine produced in plants was demonstrated as a candidate vaccine since it elicited neutralizing antibodies against A/H5N1 virus. In this study, immunogenicity and protective ability of a plant-based recombinant HA antigen which was fused to IgMFc to form oligomerized HA antigen (H5TG oligomer) had been evaluated by vaccination in chickens. Chicken sera after each vaccination were collected for Western blot, ELISA and HI assays. Ten days after the second vaccination, the chickens have been challenged with A/duck/TG/NAVET(3)/2013 virus, clade 1.1. The analysis results showed that the oligomeric recombinant H5TG antigen elicited stronger H5TG-specific IgY antibodies and A/H5N1 clade 1.1 virus-neutralizing antibodies than the H5TGpII trimeric recombinant antigen without fusing IgMFc in vaccinated chickens. Notably, the chicken protection rate against A/H5N1 clade 1.1 virus of the H5TG oligomer antigen was 80% that is not significantly lower than that of a commercial vaccine as a positive control from National Veterinary Joint Stock company NAVETCO, Vietnam with the chicken protective rate of 90%. Whereas the chicken protection rate against A/H5N1 clade 1.1 virus of the H5TG trimer antigen was 50%. These results suggest that the IgMFc motif plays an important role in the forming of oligomeric proteins which had been proved for enhancing immunogenicity and protection ability in this study. Therefore, the plant-based oligomerized recombinant H5TG antigen is a potential vaccine candidate against A/H5N1 influenza virus in the future.
Production of monoclonal antibody against Ompa protein of Candidatus Liberibacter asiaticus causing citrus greening disease
Authors: Thuong Ho Thi , Thom Nguyen Thi , Tra Nguyen Thi , Vy Trinh Thai , Ngoc Pham Bich , Son Le Van , Ngoc Nguyen Thi Bich , Chu Ha Hoang
Published: November 2020

Citrus Greening, also known as HuangLongbing (HLB), is considered one of the most dangerous citrus diseases, and limiting the production of citrus trees all over the world. Production of antibodies against Ompa protein of Candidatus Liberibacter asiaticus (CLas) for detection of citrus greening disease is considered as promising research direction. In this study, for the purpose of producting antibodies against Ompa of CLas, we firstly used the camel VHH antibody library for screening VHH antibodies against Ompa using phage-display technique. Next, phages which had strong interaction with Ompa as shown in ELISA were selected for phagemid isolation and the DNA fragments encoding VHH antibodies were sequenced. The DNA fragment encoding the best VHH antibody was then selected and inserted into the expression vector pET-21a (+), then cloned in Ecoli DH5α strain and expressed in BL21 (DE3) strain. The expression of VHH antibodies against Ompa was optimized at different temperatures with an inductive concentration of 0.1 M IPTG. Anti-Ompa VHH antibodies were purified under denatured conditions then re-folded. The biological activity of the VHH antibody with Ompa antigen was assessed by indirect-ELISA reaction. Results indicated that the VHH antibody had a very strong interaction with the Ompa antigen. This opens up the prospect of applying VHH antibody in the detection of citrus greening disease.
Authentication of the green monkey kidney Vero76 cell lines subcultured through many generations
Authors: Lien Nguyen Thi Kim , Van Tung Nguyen , Huy Hoang Nguyen , Thi Mai Huong Nguyen , Thi Van Quynh Nguyen , Van Hung Pham
Published: November 2020

The Vero76 cell lines isolated from the kidney of an African green monkey (Chlorocebus aethiops sabaeus), are commonly used for viral culture, vaccine development, and cytotoxicity assays. However, subculturation of cell lines over multiple generations can lead to a genetic change in these cell lines compared to the original cell. This will lead to deviations in results of research that using the modified cell lines. Therefore, identification and evaluation periodically the genetic stability of cell lines after subculture are necessary. In recently studies, cell authentication by using STR markers (short tandem repeat markers) have been becoming a simple and effective method that used commonly. In this study, we used eight STR markers: D17S1304, D5S1467, D19S245, D1S518, D8S1106, D4S2408, D6S1017, and DYS389 for cell lines authentication and evaluation of the genetic stability of subcultured Vero76 cell lines. The STR indicators were amplified, sequenced and compared with the original cell to identify the differences between cell lines in subcultured generations. The evaluation results showed that these cell lines did not have any differ compared with the original cell line, this confirmed that the genetic of the cell lines were stability. The results also suggested that STR markers can be used to authenticate for Vero76 cell lines.