bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in Melatonin Concentration in a Clinical Observation Study under the Influence of Low-Frequency Magnetic Fields (Magnetic Stimulation in Men with Low Back Pain)—Results of Changes in an Eight-Point Circadian Profile
Authors: Woldańska-Okońska Marta , Kubsik-Gidlewska Anna , Koszela Kamil
Published: November 2023

The aim of this study was to assess the changes in melatonin concentration under the influence of magnetic stimulation in men with low back pain. A total of 15 men were used in this study, divided into two groups. In Group 1, consisting of seven men, the M1P1 Viofor JPS program was used twice a day for 8 min, at 08:00 and 13:00. In Group 2, consisting of eight men, the M2P2 Viofor JPS program was used once a day for 12 min at 10:00. The application was subjected to the whole body of patients. The treatments in both groups lasted 3 weeks, for 5 days each week, with breaks on weekends. The diurnal melatonin profile was determined the day before exposure and the day after the last treatment, as well as at one-month follow-up. Blood samples were collected eight times a day. In both programs, magnetic stimulation did not reduce the nocturnal peak of melatonin concentration. After exposure, prolonged secretion of melatonin was observed until the morning hours. The impact of the magnetic field was maintained 1 month after the end of the application. The effect of the magnetic field was maintained for 1 month from the end of the application, which confirms the thesis about the occurrence of the phenomenon of biological hysteresis. The parameters of the magnetic fields, the application system, and the time and length of the application may affect the secretion of melatonin.
Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient
Authors: da Silva Fernanda F. , Lupinacci Fernanda C. S. , Elias Bruno D. S. , Beserra Adriano O. , Sanematsu Paulo , Roffe Martin , Kulikowski Leslie D. , Costa Felipe D’almeida , Santos Tiago G. , Hajj Glaucia N. M.
Published: November 2023

Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults, with few effective treatment strategies. The research on the development of new treatments is often constrained by the limitations of preclinical models, which fail to accurately replicate the disease’s essential characteristics. Herein, we describe the obtention, molecular, and functional characterization of the GBM33 cell line. This cell line belongs to the GBM class according to the World Health Organization 2021 Classification of Central Nervous System Tumors, identified by methylation profiling. GBM33 expresses the astrocytic marker GFAP, as well as markers of neuronal origin commonly expressed in GBM cells, such as βIII-tubulin and neurofilament. Functional assays demonstrated an increased growth rate when compared to the U87 commercial cell line and a similar sensitivity to temozolamide. GBM33 cells retained response to serum starvation, with reduced growth and diminished activation of the Akt signaling pathway. Unlike LN-18 and LN-229 commercial cell lines, GBM33 is able to produce primary cilia upon serum starvation. In summary, the successful establishment and comprehensive characterization of this GBM cell line provide researchers with invaluable tools for studying GBM biology, identifying novel therapeutic targets, and evaluating the efficacy of potential treatments.
Transfection of Vein Grafts with Early Growth Response Factor-1 Oligodeoxynucleotide Decoy: Effects on Stem-Cell Genes and Toll-like Receptor-Mediated Inflammation
Authors: Mylonas Konstantinos S. , Peroulis Michail , Kapelouzou Alkistis
Published: November 2023

The long-term patency of vein grafts is challenged by intimal hyperplasia. We sought to explore the intricate relationships between the transcription factor Egr-1, toll-like receptors (TLRs), and stem cell genes and also assessed oligodeoxynucleotide decoys (ODNs) as a strategy to prevent vein graft failures. A total of 42 New Zealand white rabbits were fed hyperlipidemic chow and classified into three groups. A double-stranded Egr-1 ODN was synthesized and infused in vein grafts prior to anastomosis with the common carotid artery. All vein grafts were retrieved at the conclusion of the predefined experimental period. Real-time quantitative polymerase chain reaction was performed to estimate expression patterns for several genes of interest. MYD88, TLR2-4, TLR8, NF-kB, TNF-α, IFNβ, and IFNγ; chemokines CCL4, CCL20, CCR2; numerous interleukins; and stem cell genes KFL4, NANOG, HOXA5, and HIF1α were universally downregulated in the ODN arm compared with the controls. By understanding these multifaceted interactions, our study offers actionable insights that may pave the way for innovative interventions in vascular reconstructions.
The Intriguing Sigma-1 and Sigma-2 Receptors and Their Potential Therapeutic Roles 2.0
Authors: Abate Carmen , Maurice Tangui
Published: November 2023

For some time now, the research on sigma receptors has been at a high level of maturity but, despite everything that has already been achieved, further work in this field still holds huge appeal, with vast possibilities for original discoveries [...]
Predictive Potential of RNA Polymerase B (II) Subunit 1 (RPB1) Cytoplasmic Aggregation for Neoadjuvant Chemotherapy Failure
Authors: Nagy-Mikó Bence , Németh-Szatmári Orsolya , Faragó-Mészáros Réka , Csókási Aliz , Bognár Bence , Ördög Nóra , Borsos Barbara N. , Majoros Hajnalka , Ujfaludi Zsuzsanna , Oláh-Németh Orsolya , Nikolényi Aliz , Dobi Ágnes , Kószó Renáta , Sántha Dóra , Lázár György , Simonka Zsolt , Paszt Attila , Ormándi Katalin , Pankotai Tibor , Boros Imre M. , Villányi Zoltán , Vörös András
Published: November 2023

We aimed to investigate the contribution of co-translational protein aggregation to the chemotherapy resistance of tumor cells. Increased co-translational protein aggregation reflects altered translation regulation that may have the potential to buffer transcription under genotoxic stress. As an indicator for such an event, we followed the cytoplasmic aggregation of RPB1, the aggregation-prone largest subunit of RNA polymerase II, in biopsy samples taken from patients with invasive carcinoma of no special type. RPB1 frequently aggregates co-translationally in the absence of proper HSP90 chaperone function or in ribosome mutant cells as revealed formerly in yeast. We found that cytoplasmic foci of RPB1 occur in larger sizes in tumors that showed no regression after therapy. Based on these results, we propose that monitoring the cytoplasmic aggregation of RPB1 may be suitable for determining—from biopsy samples taken before treatment—the effectiveness of neoadjuvant chemotherapy.
Mitochondrial Impairment: A Link for Inflammatory Responses Activation in the Cardiorenal Syndrome Type 4
Authors: Amador-Martínez Isabel , Aparicio-Trejo Omar Emiliano , Bernabe-Yepes Bismarck , Aranda-Rivera Ana Karina , Cruz-Gregorio Alfredo , Sánchez-Lozada Laura Gabriela , Pedraza-Chaverri José , Tapia Edilia
Published: November 2023

Cardiorenal syndrome type 4 (CRS type 4) occurs when chronic kidney disease (CKD) leads to cardiovascular damage, resulting in high morbidity and mortality rates. Mitochondria, vital organelles responsible for essential cellular functions, can become dysfunctional in CKD. This dysfunction can trigger inflammatory responses in distant organs by releasing Damage-associated molecular patterns (DAMPs). These DAMPs are recognized by immune receptors within cells, including Toll-like receptors (TLR) like TLR2, TLR4, and TLR9, the nucleotide-binding domain, leucine-rich-containing family pyrin domain-containing-3 (NLRP3) inflammasome, and the cyclic guanosine monophosphate (cGMP)–adenosine monophosphate (AMP) synthase (cGAS)–stimulator of interferon genes (cGAS-STING) pathway. Activation of these immune receptors leads to the increased expression of cytokines and chemokines. Excessive chemokine stimulation results in the recruitment of inflammatory cells into tissues, causing chronic damage. Experimental studies have demonstrated that chemokines are upregulated in the heart during CKD, contributing to CRS type 4. Conversely, chemokine inhibitors have been shown to reduce chronic inflammation and prevent cardiorenal impairment. However, the molecular connection between mitochondrial DAMPs and inflammatory pathways responsible for chemokine overactivation in CRS type 4 has not been explored. In this review, we delve into mechanistic insights and discuss how various mitochondrial DAMPs released by the kidney during CKD can activate TLRs, NLRP3, and cGAS-STING immune pathways in the heart. This activation leads to the upregulation of chemokines, ultimately culminating in the establishment of CRS type 4. Furthermore, we propose using chemokine inhibitors as potential strategies for preventing CRS type 4.
miRNAs: Targets to Investigate Herpesvirus Infection Associated with Neurological Disorders
Authors: de Souza Carneiro Vanessa Cristine , Leon Luciane Almeida Amado , de Paula Vanessa Salete
Published: November 2023

Herpesvirus is associated with various neurological disorders and a specific diagnosis is associated with a better prognosis. MicroRNAs (miRNAs) are potential diagnostic and prognostic biomarkers of neurological diseases triggered by herpetic infection. In this review, we discuss miRNAs that have been associated with neurological disorders related to the action of herpesviruses. Human miRNAs and herpesvirus-encoded miRNAs were listed and discussed. This review article will be valuable in stimulating the search for new diagnostic and prognosis alternatives and understanding the role of these miRNAs in neurological diseases triggered by herpesviruses.
Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool
Authors: Savicheva Alevtina M. , Krysanova Anna A. , Budilovskaya Olga V. , Spasibova Elena V. , Khusnutdinova Tatiana A. , Shalepo Kira V. , Beliaeva Natalia R. , Safarian Galina Kh. , Sapozhnikov Kirill V. , Tapilskaya Natalya I. , Kogan Igor Yu.
Published: November 2023

Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow a complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due to the high costs and limited availability of NGS, the multiplex real-time PCR draws more attention. The present study aimed to evaluate the microbial composition and dominant lactobacilli species in non-pregnant women with bacterial vaginosis using a multiplex RT-PCR test and determine its diagnostic significance. In total, 331 women complaining of vaginal discharge were included. BV was confirmed upon clinical examination and Nugent criteria. A real-time PCR test was carried out with a new Femoflor test, which identifies opportunistic bacteria, STD pathogens, and some viruses. According to the results, the rate of lactobacilli is significantly reduced in BV-affected patients when compared to healthy women. Moreover, the rate of L. crispatus significantly decreases, while the rate of L. iners remains high. Among obligate anaerobic bacteria, Gardnerella vaginalis was the most prevalent in women with BV. The Femoflor test demonstrated high sensitivity and specificity for diagnosing BV. Moreover, the test allows the identification of infection in women with intermediate vaginal microbiota, as well as STD pathogens, and viruses. Thus, the application of real-time PCR tests can be effectively used in vaginal microbiota evaluation in women with BV, intermediate vaginal microbiota, and healthy women. In addition, this test may be used as an alternative to the Amsel criteria and Nugent scoring method in diagnosing BV.
Machine Learning in the Analysis of Carbon Dioxide Flow on a Site with Heterogeneous Vegetation
Authors: Kulakova Ekaterina , Muravyova Elena
Published: November 2023

The article presents the results of studies of carbon dioxide flow in the territory of section No. 5 of the Eurasian Carbon Polygon (Russia, Republic of Bashkortostan). The gas analyzer Sniffer4D V2.0 (manufactured in Shenzhen, China) with an installed CO2 sensor, quadrocopter DJI MATRICE 300 RTK (manufactured in Shenzhen, China) were used as control devices. The studies were carried out on a clear autumn day in conditions of green vegetation and on a frosty November day with snow cover. Statistical characteristics of experimental data arrays are calculated. Studies of the influence of temperature, humidity of atmospheric air on the current value of CO2 have been carried out. Graphs of the distribution of carbon dioxide concentration in the atmospheric air of section No. 5 on autumn and winter days were obtained. It has been established that when building a model of CO2 in the air, the parameters of the process of deposition by green vegetation should be considered. It was found that in winter, an increase in air humidity contributes to a decrease in gas concentration. At an ambient temperature of 21 °C, an increase in humidity leads to an increase in the concentration of carbon dioxide.
Multiple Information-Aware Recurrent Reasoning Network for Joint Dialogue Act Recognition and Sentiment Classification
Authors: Li Shi , Chen Xiaoting
Published: November 2023

The task of joint dialogue act recognition (DAR) and sentiment classification (DSC) aims to predict both the act and sentiment labels of each utterance in a dialogue. Existing methods mainly focus on local or global semantic features of the dialogue from a single perspective, disregarding the impact of the other part. Therefore, we propose a multiple information-aware recurrent reasoning network (MIRER). Firstly, the sequence information is smoothly sent to multiple local information layers for fine-grained feature extraction through a BiLSTM-connected hybrid CNN group method. Secondly, to obtain global semantic features that are speaker-, context-, and temporal-sensitive, we design a speaker-aware temporal reasoning heterogeneous graph to characterize interactions between utterances spoken by different speakers, incorporating different types of nodes and meta-relations with node-edge-type-dependent parameters. We also design a dual-task temporal reasoning heterogeneous graph to realize the semantic-level and prediction-level self-interaction and interaction, and we constantly revise and improve the label in the process of dual-task recurrent reasoning. MIRER fully integrates context-level features, fine-grained features, and global semantic features, including speaker, context, and temporal sensitivity, to better simulate conversation scenarios. We validated the method on two public dialogue datasets, Mastodon and DailyDialog, and the experimental results show that MIRER outperforms various existing baseline models.
First Optimization of Tomato Pomace in Diets for Tenebrio molitor (L.) (Coleoptera: Tenebrionidae)
Authors: Baldacchino Ferdinando , Spagnoletta Anna , Lamaj Flutura , Vitale Maria Luisa , Verrastro Vincenzo
Published: November 2023

Tomato pomace (TP), an agricultural industrial waste product from the tomato processing industry, is valorized as a rearing substrate for Tenebrio molitor (L.). This study evaluated bran-based diets with increasing tomato pomace (0%, 27%, 41%, and 100%). Protein sources, such as brewer’s spent grain and yeast, were used in TP27 and TP41 diets to ensure equal protein contents to the control diet. Results showed no different for larval and pupal weights between diets; however, the time of development significantly increases in TP100 compared to all diets. The feed conversion rate progressively increases from 2.7 to 4.3, respectively, from the control to the TP100 diet. Conversely, lycopene and β-carotene increase in the larvae. The fatty acid composition improves by increasing polyunsaturated fatty acids (mainly α-linoleic acid). Although the best nutritional quality was obtained in T100, the TP41 is the optimal diet for balance between larval performance and qualitative improvement of larvae. Therefore, tomato pomace is suitable for the formulation of mealworm diets, even in high dosages, when supplemented with sustainable protein and carbohydrate sources.
Percutaneous Cryoablation under Conscious Sedation: A Safe, Effective and Painless Option for the Treatment of Pediatric Osteoid Osteoma
Authors: Pusceddu Claudio , Vergantino Elva , Santucci Domiziana , Marsico Salvatore , Cappucci Matteo , Vaccarino Federica , Beomonte Zobel Bruno , Grasso Rosario Francesco , Faiella Eliodoro
Published: November 2023

Background: Percutaneous CT-guided cryoablation is an emerging technique for treating bone tumors. However, experience with using this procedure for osteoid osteomas in pediatric patients remains limited. Our study aims to assess its technical feasibility, clinical efficacy, and safety in children treated under conscious anesthesia. Methods: We conducted a retrospective study of consecutive pediatric patients who underwent CT-guided percutaneous cryoablation for osteoid osteomas at our institution between September 2017 and March 2021. All patients received conscious anesthesia. Data on peri-procedural VAS scores, post-procedural VAS scores, imaging findings, and nonsteroidal anti-inflammatory drug (NSAID) usage rates were collected for each patient. Technical success was defined as proper cryoprobe placement at the nidus center, while clinical success referred to pain relief without NSAID use. Intra- and post-operative complications were also evaluated. Results: Nine patients underwent CT-guided percutaneous cryoablation for osteoid osteomas under conscious sedation, with a 100% overall success rate with low peri-procedural and median VAS scores (p < 0.01). No complications were observed during or after the procedure. Conclusions: CT-guided percutaneous cryoablation of pediatric osteoid osteomas is an effective and safe minimally invasive procedure feasible under conscious anesthesia, holding promise as a valuable treatment option.
Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp
Authors: dos Santos Fernanda Furuse Ventura , Habelitz Stefan , Nascimento Fábio Dupart , Arana-Chavez Victor Elias , Braga Roberto Ruggiero
Published: November 2023

In recent years, alternative pulpal therapies targeting dentinogenesis signaling pathways using different peptides have been investigated. The aim of this study was to verify the effectiveness of poly(aspartic acid), pAsp, in dentin regeneration using an animal model. Methods: Mechanical pulp exposure was performed in the upper molars of 56 Wistar rats, randomly divided as follows (n = 14): control (no treatment); MTA group—pulp capping with mineral trioxide aggregate (MTA Angelus); pAsp group—application of 20 μL of pAsp solution (25 mg·mL−1); MTA+pAsp group—application of MTA mixed with pAsp (5:1 by mass). Animals were euthanized after 7 or 21 days. Histological sections were submitted to hematoxylin-eosin and Brown and Brenn staining and immunohistochemical analysis for osteopontin (OPN) and dentin matrix protein 1 (DMP 1). Results: At 7 days, an acute inflammatory infiltrate and the presence of disorganized mineralized tissue were observed in all groups. At 21 days, the quality and thickness of the reparative dentin in treated groups were superior to the control, and bacterial contamination was observed in two MTA-pAsp specimens. While all treated groups showed intense immunostaining for OPN at 21 days, only the pAsp group expressed DMP 1, indicating the presence of fully differentiated odontoblast-like cells. Conclusion: Poly(aspartic) acid promoted dentin regeneration in rat molars in the absence of an additional calcium source and may be an alternative to MTA as a pulp-capping agent.
A Single, Multimodal Exercise Tolerance Test Can Assess Combat Readiness in Army-ROTC Cadets: A Brief Report
Authors: Crawford Derek A. , Heinrich Katie M. , Haddock Christopher K. , Poston W. S. Carlos , Day R. Sue , Kaipust Christopher , Skola Blake , Wakeman Amanda J. , Kunkel Eric , Bell Addison , Wilhite Emily , Young Nathanial , Whitley Allison , Fritts Madelyn
Published: November 2023

The Army Combat Fitness Test (ACFT) is a multi-event assessment battery designed to determine the combat readiness of U.S. Army personnel. However, for Reserve Officers’ Training Corps (ROTC) programs the logistical demands of collegiate life make repeated administration of the ACFT challenging. The present study sought to design and evaluate a single, multimodal exercise tolerance test (METT) capable of serving as a time-efficient proxy measure of combat readiness. Methods: Using a formal instrument design process, we constructed the METT to mimic the demands of the ACFT and assessed its reliability, validity, and responsiveness. Results: The METT demonstrates minimal measurement error (i.e., a 2% coefficient of variation), concurrent validity with the ACFT (R2 = 0.327, F = 10.67, p < 0.001), the ability to classify cadets who may be at-risk for failing the ACFT (X2 = 8.16, p = 0.017, sensitivity = 0.878, specificity = 0.667), and appropriate change following a training intervention (5.69 ± 8.9%). Conclusions: The METT has the potential to provide a means to monitor progress, identify areas for improvement, and guide informed decision-making regarding individualization of cadet combat training plans.
Measuring Perceived Voice Disorders and Quality of Life among Female University Teaching Faculty
Authors: Al Awaji Nisreen Naser , Alghamdi Khaled Abdulraheem , Alfaris Abdullah Mohammed , Alzamil Rahaf Zamil , Alhijji Lojain Naser , Alyehya Ghaida Saad , Al Harbi Shadan Mohammed , Mortada Eman M.
Published: November 2023

Background: Occupations that require heavy vocal use can place the person at risk of voice disorders (VDs). Heavy demands on the voice, especially for a long time or with loud back-ground noise, can lead to vocal abuse or misuse. The study aimed to measure the prevalence of perceived voice disorders among the teaching faculty at a female university, identify the risk fac-tors that affect their voice, and determine the effect of perceived voice disorders on their quality of life (QoL). Methods: The study sample consisted of female teaching faculty (N = 401). The ques-tionnaire included general sociodemographic data, general voice data, the vocal tract discomfort (VTD) scale, and the World Health Organization Quality of Life assessment (WHOQOL)-BREF. Results: The results demonstrated that 44.1% of the participants had perceived voice disorders, and stress, reflux, and asthma had a significant relationship with self-perceived voice disorders. Furthermore, the data showed that self-perceived voice disorders negatively impacted the overall QoL of teaching faculty. Conclusions: Perceived voice disorders are affected by various factors, including health conditions, medications, and lifestyle choices. Although teaching characteristics and demo-graphic factors are believed to be the cause, in this study they did not significantly contribute to perceived voice disorders. Faculty members with perceived voice disorders have a poorer quality of life, highlighting the need for education on preventative vocal measures and awareness of voice care.
An Improved Denoising Method for Fault Vibration Signals of Wind Turbine Gearbox Bearings
Authors: Zhang Chaohai , Zhang Xu , Xu Zufeng , Dai Wei , Lu Jie
Published: November 2023

Vibration monitoring (VM) is an important tool for fault diagnosis in key components of wind turbine gearboxes (WTGs). However, due to the influence of white noise and random interference, it is difficult to realize high-quality denoising of WTG-VM signals. To overcome this limitation, a novel joint denoising method for fault WTG-VM signals is proposed in this article, which we have named EWTKC-SVD. First, the empirical wavelet transform (EWT) boundary exploration method is used to optimize frequency band allocation and obtain the multiple intrinsic mode functions (IMFs). Second, the sensitive IMFs are selected according to the calculated correlation coefficient and kurtosis index, avoiding IMF redundancy. Finally, the fault WTG-VM signals are obtained using SVD denoising. Using this approach, the proposed method realizes high-quality denoising of WTG-VM signals. Furthermore, it also effectively solves the existing problems of conventional methods, namely, inefficient IMF selection, high noise, false frequencies, mode mixing, and end effect. Finally, the effectiveness, superiority, and reliability of the proposed method are proved using simulation and practical case results.
Oral Manifestations of Graft vs. Host Disease: A Comprehensive Review for Best Practice in Dentistry
Authors: Miranda Michele , Gianfreda Francesco , Carlotta Danesi , Armati Sofia , Barlattani Alberta , Bollero Patrizio
Published: November 2023

Graft-versus-host disease (GVHD) is a complication of hematopoietic stem cell transplantation (HSCT). GVHD may also develop following solid transplants or blood transfusions if white blood cells are transferred. GVHD affects multiple organs, including the oral tissues. This pictorial review provides a background of GVHD to dental practitioners, describes the most common oral manifestations of GVHD, and highlights the main treatment modifications needed to deliver dental care to patients with GVHD. A narrative review enriched with clinical data was performed by searching the scientific literature for all articles regarding GVHD and oral manifestations/therapies. All articles without exclusion criteria, except animal tests, were included in the above review. Acute GVHD may manifest in the oral mucosa; however, it often develops immediately following HSCT when routine dental treatment is postponed. Chronic GVHD may manifest in the oral mucosa, the salivary glands, and the musculoskeletal compartment. It may indirectly affect the teeth and the oral flora, putting the patient at risk for infections. Importantly, GVHD poses an increased risk for oral cancer. GVHD has a wide range of oral manifestations, some of which may affect dental treatment.
Implementation of the Baveno Classification in Obstructive Sleep Apnea and Its Correlation with Symptoms of Anxiety and Depression
Authors: Suša Romana , Ratinac Miloš , Ćupurdija Vojislav , Novković Ljiljana , Milojević-Ilić Mirjana , Petrović Marina , Igrutinović Nebojša , Vuleta Marko , Timotijević Ljiljana , Kostić Olivera , Čekerevac Ivan
Published: November 2023

Background and Objectives: The Baveno classification represents a new approach to the assessment of the severity of OSA (Obstructive sleep apnea), which takes significant comorbidities into account: atrial fibrillation, arterial hypertension, heart failure, stroke, diabetes mellitus, and OSA symptoms expressed through the Epworth sleepiness scale (ESS). The authors believe that the Baveno classification facilitates a better stratification of patients with OSA and can be a good guide for deciding on the therapeutic approach and clinical monitoring of patients with OSA, compared to the AHI (apnea-hypopnea index) itself. The aim of this paper is to confirm the advantage of applying the Baveno classification to the evaluation of symptoms of anxiety and depression in the OSA patients compared to the application of the AHI as a single parameter. Materials and Methods: This research represents an observational retrospective study that was performed at the Pulmonology Clinic of the University Clinical Center in Kragujevac, Serbia. The study sample included 104 patients with diagnosed OSA. Patients were divided into four categories retrogradely according to the Baveno classification (A, B, C, and D). Statistical data processing was performed using the IBM SPSS Statistics version 25.0 program. Results: In our study, we proved that the Baveno classification is better at predicting the depressive disorder in OSA patients compared to the AHI itself, according to abnormal BDI-II (Beck Depression Inventory) score (value greater than ten) and HADS-D (Hospital anxiety and depression) scale (value greater than eight). The average AHI in the entire group of examined patients was 44.3 ± 19.8, while in category A the average AHI was 25.2 ± 10, in category B, 53.4 ± 20.6; in category C, 38.2 ± 18.5; and in category D, 48.1 ± 19.2. In the total sample, AHI did not correlate with the depressive episodes, but individually, the highest frequency of the depressive symptoms was precisely in the categories with the highest AHI (group D and B), where more than half of the subjects had an abnormal score. The frequency of the anxiety disorder (HADS-A) between the analyzed groups did not differ significantly, although the largest number of patients with significant anxiety were in category B, according to the Baveno classification. Conclusions: We proved that the Baveno classification is applicable in real life, and it is better at evaluating anxiety and depression using questionnaires and can identify new patients who need CPAP therapy, independently of other OSAS symptoms, primarily daytime sleepiness.
Nursing Students’ Knowledge and Attitudes toward Danger Signs in Neonatal Illnesses
Authors: Eltyeb Ebtihal , Basheer Randa , Ahmed Amel , Al-Makramani Ali , Mahfouz Mohamed Salih , Mohamedali Amna , Balla Anwar , Algadi Halima , Gohal Gassem
Published: November 2023

Background and Objectives: Neonates can show subtle signs of illness that could be overlooked by their mothers and caregivers. Therefore, basic knowledge regarding neonatal health and early detection of neonatal diseases can help improve survival. We assessed nursing students’ knowledge and attitudes toward the danger signs of neonatal illnesses. Materials and Methods: We performed a descriptive cross-sectional study using a structured online questionnaire based on danger signs categorized by the World Health Organization to assess knowledge of neonatal illness danger signs among nursing students. Results: We assessed 342 students, of which 67.0% (95% CI: 61.8–71.7) had good knowledge regarding neonatal illness danger signs, and 71.6% received information about neonatal care. About 33% of the participants had a basic knowledge of neonatal care, such as breastfeeding, immunization, routine postnatal care, and eye care. Students who received information on neonatal care were three times (AOR = 2.95, 95% CI: 11.59–5.47, p = 0.001) more likely to have good knowledge than those who did not. The students also showed a positive attitude towards the importance of knowledge regarding neonatal illness danger signs, teaching this subject in their college, participating in studies involving the practice and knowledge of mothers regarding neonatal illness danger signs, and the importance of knowledge in reducing neonatal mortality in the region. Conclusions: More training programs on neonatal care should be implemented to enhance knowledge and raise nursing students’ awareness of the dangerous signs of neonatal illnesses.
Electrometric and Electron Paramagnetic Resonance Measurements of a Difference in the Transmembrane Electrochemical Potential: Photosynthetic Subcellular Structures and Isolated Pigment–Protein Complexes
Authors: Semenov Alexey Yu. , Tikhonov Alexander N.
Published: November 2023

A transmembrane difference in the electrochemical potentials of protons (ΔμH+) serves as a free energy intermediate in energy-transducing organelles of the living cell. The contributions of two components of the ΔμH+ (electrical, Δψ, and concentrational, ΔpH) to the overall ΔμH+ value depend on the nature and lipid composition of the energy-coupling membrane. In this review, we briefly consider several of the most common instrumental (electrometric and EPR) methods for numerical estimations of Δψ and ΔpH. In particular, the kinetics of the flash-induced electrometrical measurements of Δψ in bacterial chromatophores, isolated bacterial reaction centers, and Photosystems I and II of the oxygenic photosynthesis, as well as the use of pH-sensitive molecular indicators and kinetic data regarding pH-dependent electron transport in chloroplasts, have been reviewed. Further perspectives on the application of these methods to solve some fundamental and practical problems of membrane bioenergetics are discussed.
Influence of Intramineral Proteins on the Growth of Carbonate Crystals Using as a Scaffold Membranes of Ratite Birds and Crocodiles Eggshells
Authors: Elejalde-Cadena Nerith R. , Hernández Denisse , Capitelli Francesco , Islas Selene R. , Rosales-Hoz Maria J. , Zema Michele , Tarantino Serena C. , Siliqi Dritan , Moreno Abel
Published: November 2023

The lack of information on structural basis where proteins are involved, as well as the biomineralization processes of different systems such as bones, diatom frustules, and eggshells, have intrigued scientists from different fields for decades. This scientific curiosity has led to the use of methodologies that help understand the mechanism involved in the formation of these complex structures. Therefore, this work focuses on the use of eggshell membranes from different species of ratites (emu and ostrich) and reptiles (two species of crocodiles) as a model to differentiate biocalcification and biosilicification by introducing calcium phosphate or silica inside the membrane fiber mantles. We performed this to obtain information about the process of eggshell formation as well as the changes that occur in the membrane during crystal formation. In order to identify and understand the early processes leading to the formation of the microstructures present in the eggshell, we decided to carry out the synthesis of silica-carbonate of calcium, barium, and strontium called biomorph in the presence of intramineral proteins. This was carried out to evaluate the influence of these proteins on the formation of specific structures. We found that the proteins on untreated membranes, present a structural growth similar to those observed in the inner part of the eggshell, while in treated membranes, the structures formed present a high similarity with those observed in the outer and intermediate part of the eggshell. Finally, a topographic and molecular analysis of the biomorphs and membranes was performed by scanning electron microscopy (SEM), Raman and Fourier-transform Infrared (FTIR) spectroscopies.
Inverse Identification of Drucker–Prager Cap Model for Ti-6Al-4V Powder Compaction Considering the Shear Stress State
Authors: Li Runfeng , Liu Wei , Li Jiaqi , Liu Jili
Published: November 2023

Numerical simulation is an important method to investigate powder-compacting processes. The Drucker–Prager cap constitutive model is often utilized in the numerical simulation of powder compaction. The model contains a number of parameters and it requires a series of mechanical experiments to determine the parameters. The inverse identification methods are time-saving alternatives, but most procedures use a flat punch during the powder-compacting process. It does not reflect the densification behavior under a shearing stress state. Here, an inverse identification approach for the Drucker–Prager cap model parameters is developed by using a hemispherical punch for the powder-compacting experiment. The error between the numerical and experimental displacement–load curves was minimized to identify the Drucker–Prager cap model of titanium alloy powder. The identified model was then verified by powder-compacting experiments with the flat punch. The displacement–load curves acquired by numerical simulation were compared to the displacement–load curves obtained through experiments. The two curves are found to be in good agreement. Meanwhile, the relative density distribution of the powders is similar to the experimental results.
Study on the Properties of a 2219 Aluminum Alloy Friction Stir-Welded Joint under the Biaxial Stress State
Authors: Shao Minghao , Wang Di , Huang Cheng , Chang Xin
Published: November 2023

Friction stir welding (FSW) has been considered ideal for aluminum alloy structures. The performance of FSW joints under unidirectional stress state has been widely tested, but there is still a lack of experimental data under a biaxial stress state. For accurate characterization of the mechanical properties of 2219 aluminum alloy FSW joints under a biaxial stress state, this article conducted biaxial tests. The FSW joint was tested using scanning electron microscopy (SEM) to obtain its microscopic properties and obtain the partition results of the welded joint. The stress–strain relationship and yield characteristics of welded joints under different loading ratios were studied using biaxial tensile tests of cruciform specimens combined with digital image correlation technology. By comparing it with the existing yield criteria, the yield criterion suitable for FSW joints of 2219 aluminum alloy under a biaxial tensile load was determined.
Benchmark Dataset for Training Machine Learning Models to Predict the Pathway Involvement of Metabolites
Authors: Huckvale Erik D. , Powell Christian D. , Jin Huan , Moseley Hunter N. B.
Published: November 2023

Metabolic pathways are a human-defined grouping of life sustaining biochemical reactions, metabolites being both the reactants and products of these reactions. But many public datasets include identified metabolites whose pathway involvement is unknown, hindering metabolic interpretation. To address these shortcomings, various machine learning models, including those trained on data from the Kyoto Encyclopedia of Genes and Genomes (KEGG), have been developed to predict the pathway involvement of metabolites based on their chemical descriptions; however, these prior models are based on old metabolite KEGG-based datasets, including one benchmark dataset that is invalid due to the presence of over 1500 duplicate entries. Therefore, we have developed a new benchmark dataset derived from the KEGG following optimal standards of scientific computational reproducibility and including all source code needed to update the benchmark dataset as KEGG changes. We have used this new benchmark dataset with our atom coloring methodology to develop and compare the performance of Random Forest, XGBoost, and multilayer perceptron with autoencoder models generated from our new benchmark dataset. Best overall weighted average performance across 1000 unique folds was an F1 score of 0.8180 and a Matthews correlation coefficient of 0.7933, which was provided by XGBoost binary classification models for 11 KEGG-defined pathway categories.
Constraints on Crystallinity of Graphite Inclusions in Nephrite Jade from Xinjiang, Northwest China: Implications for Nephrite Jade Formation Temperatures
Authors: Zheng Jifei , Chen Lei , Zhang Cun , Liu Yue , Tian Ruicong , Wu Jinlin , Wu Yu , Zhang Shouting
Published: November 2023

Graphite usually occurs in mineral/rock associations in the form of solid inclusions and plays an important role in tracing regional metamorphic degree, ore-forming temperature, fluid evolution, as well as the deep carbon cycle of the Earth. In this study, we investigate the placer black nephrite jade where the co-occurrence of abundant graphite inclusions and jade remains extraordinary. By employing petrographic, mineral-chemical, and Raman spectroscopic methods, we characterize the textures and crystallinity of graphite inclusions that exist in nephrite jade. EPMA and petrological data indicate that the main constituents of black jade are tremolite and graphite, with minor phases of diopside, calcite, dolomite, epidote, and apatite. Micro-Raman spectroscopic thermometry of carbonaceous material shows that most of the formation temperatures of graphite inclusions are between 378 and 556 °C, and only a few temperatures may be above 650 °C, indicating that graphite inclusions were formed at medium- to high-temperature metamorphic facies. The petrologic and spectral investigations of graphite inclusions in these nephrite jade samples show major metamorphic signatures with mixed features associated with fluid precipitation. Our results allow us to propose that primary nephrite jade was formed under multi-stage tectonic evolution conditions, and regional temperatures were predominately driven by the late continent–continent collision, while the ore-controlling temperatures of nephrite jade formation were found in a medium- to high-temperature environment.
Design and Fabrication of High Performance InGaAs near Infrared Photodetector
Authors: Liu Hezhuang , Wang Jingyi , Guo Daqian , Shen Kai , Chen Baile , Wu Jiang
Published: November 2023

InGaAs photodiodes have a wide range of important applications; for example, NIR imaging, fiber optical communication, and spectroscopy. In this paper, we studied InGaAs photodiodes with different doping concentration absorber layers. The simulated results suggested that, by reducing the absorber doping concentration from 1 × 1016 to 1 × 1015 cm−3, the maximum quantum efficiency of the devices can rise by 1.2%, to 58%. The simulation also showed that, by increasing the doping concentration of the absorber layer within a certain range, the dark current of the device can be slightly reduced. A PIN structure was grown and fabricated, and CV measurements suggested a low doping concentration of about 1.2 × 1015 cm−3. Although the thermal activation energy of the dark current suggested a distinct component of shunt dark current at a high temperature range, a dark current of ~6 × 10−4 A/cm2 (−0.5 V) was measured at room temperature. The peak quantum efficiency of the InGaAs device was characterized as 54.7% without antireflection coating and 80.2% with antireflection coating.
Enhancing Cache Robustness in Information-Centric Networks: Per-Face Popularity Approaches
Authors: Baugh John , Guo Jinhua
Published: November 2023

Information-Centric Networking (ICN) is a new paradigm of network architecture that focuses on content rather than hosts as first-class citizens of the network. As part of these architectures, in-network storage devices are essential to provide end users with close copies of popular content, to reduce latency and improve the overall experience for the user but also to reduce network congestion and load on the content producers. To be effective, in-network storage devices, such as content storage routers, should maintain copies of the most popular content objects. Adversaries that wish to reduce this effectiveness can launch cache pollution attacks to eliminate the benefit of the in-network storage device caches. Therefore, it is crucial to protect these devices and ensure the highest hit rate possible. This paper demonstrates Per-Face Popularity approaches to reducing the effects of cache pollution and improving hit rates by normalizing assessed popularity across all faces of content storage routers. The mechanisms that were developed prevent consumers, whether legitimate or malicious, on any single face or small number of faces from overwhelmingly influencing the content objects that remain in the cache. The results demonstrate that per-face approaches generally have much better hit rates than currently used cache replacement techniques.
Comparison of the Transduction Capacity of AAV5 and AAV PHP.eB Serotypes in Hippocampus Astroglia
Authors: Borodinova Anastasia , Ierusalimsky Victor , Balaban Pavel
Published: November 2023

In the present study, we compared the astrocyte-transducing potential of the relatively novel engineered AAV PHP.eB serotype and the well-examined conventional AAV5 serotype. We generated the AAV-based genetic constructs with membrane-bound fluorescent markers under the control of the astroglial promoter GfaABC1D to target astrocytes in vivo, either via local injection into the hippocampus (AAV5, AAV PHP.eB) or via systemic injection in the retro-orbital venous sinus (AAV PHP.eB). We collected new data on the transduction properties of locally injected PHP.eB and AAV5 viruses. A morphological examination and immunostainings of mouse brain slices revealed a dose-dependent shift of cellular tropism for locally injected PHP.eB from astroglial to astroglial-neuronal as the concentration increased. When the high doses of PHP.eB viruses were administered systemically, we observed strong astrocyte transduction throughout the brain, as confirmed by the morphological examination and GFAP immunostaining. AAV5 exhibited consistent astrocytic expression in all tested concentrations. The obtained results suggest that AAV5 is more suitable for astrocyte targeting in routine stereotaxic viral injection experiments. The widely used engineered PHP.eB capsid was originally designed for the transduction of both neurons and glia. Dual cellular tropism of PHP.eB viruses, observed using different doses and different delivery protocols (local vs. systemic), suggests that the usage of AAV5 is more reliable for astrocyte labeling and that intrahippocampal injection is more suitable than systemic injection for the preferential labeling of hippocampal astroglia.
An Advanced Review: Polyurethane-Related Dressings for Skin Wound Repair
Authors: Liang Wenzi , Ni Na , Huang Yuxin , Lin Changmin
Published: November 2023

The inability of wounds to heal effectively through normal repair has become a burden that seriously affects socio-economic development and human health. The therapy of acute and chronic skin wounds still poses great clinical difficulty due to the lack of suitable functional wound dressings. It has been found that dressings made of polyurethane exhibit excellent and diverse biological properties, but lack the functionality of clinical needs, and most dressings are unable to dynamically adapt to microenvironmental changes during the healing process at different stages of chronic wounds. Therefore, the development of multifunctional polyurethane composite materials has become a hot topic of research. This review describes the changes in physicochemical and biological properties caused by the incorporation of different polymers and fillers into polyurethane dressings and describes their applications in wound repair and regeneration. We listed several polymers, mainly including natural-based polymers (e.g., collagen, chitosan, and hyaluronic acid), synthetic-based polymers (e.g., polyethylene glycol, polyvinyl alcohol, and polyacrylamide), and some other active ingredients (e.g., LL37 peptide, platelet lysate, and exosomes). In addition to an introduction to the design and application of polyurethane-related dressings, we discuss the conversion and use of advanced functional dressings for applications, as well as future directions for development, providing reference for the development and new applications of novel polyurethane dressings.
Gender Differences, Trauma, and Resilience of Children Born of Rape, and Perception of Their Behavior by Parents and the Community in the East of the Democratic Republic of Congo
Authors: Foussiakda Cécilia A. , Gavray Claire , Mugumaarhahama Yannick , Balegamire Juvenal B. , Blavier Adelaïde
Published: November 2023

This study was conducted in the eastern DR Congo to analyze the trauma of children born of rape (CBOR), and their behavior as it is perceived by their parents and community. Twenty-four families of women rape survivors and twenty-seven control families were used. The Trauma Symptoms Checklist for Children, Child Behavior Checklist, and Child and Youth Resilience Measure tests were applied. In addition, a discussion group was conducted with community members. Comparatively to girls, boys born from rape are traumatized and have psychopathological concerns such as anxiety, depression, and summation, and high internalized and externalized behaviors compared to boys from control families. Furthermore, CBOR are aggressive and gather in gangs. Despite the suffering, both CBOR and their siblings increase their resilience over the years and derive it from their environment, especially in the absence of the father who has become a polygamist. Girls born of rape are more resilient than their siblings.