bluuebunny/crossref_metadata_2025 huggingface dataset:
Sex- and Age-Specific Centile Curves and Downloadable Calculator for Clinical Muscle Strength Tests to Identify Probable Sarcopenia
Authors: Warden Stuart J , Liu Ziyue , Moe Sharon M
Published: December 2021

Objective

Identifying muscle weakness and probable sarcopenia using strength tests requires reference data. This study aimed to provide age- and sex-specific normative data for grip strength and common variations of the Sit-to-Stand (STS) test: time to complete 5 stands (5x-STS) and number of stands completed in 30 seconds (30s-STS). Predictors of test performance were also explored.

Methods

Dominant hand grip strength was assessed in adults (age = 18–80 years) using a digital dynamometer, and 5x-STS and 30s-STS performance were assessed synchronously during a single 30-second test. Sex-specific centile curves were generated using the lambda-mu-sigma method.

Results

Data from 2301 participants (female = 1682, male = 619) were included. Peak median grip strength occurred in female participants at 33.9 years of age (27.9 kg) and in male participants at 37.6 years of age (47.2 kg). 5x-STS and 30s-STS performance peaked at the youngest age (18.0 years) in both female participants (8.16 seconds and 17.2 repetitions) and male participants (8.02 seconds and 17.7 repetitions). Test performances were lowest for all tests at the oldest age in the database. Predictors of better test performance included lower age and higher self-reported physical functioning and appendicular skeletal muscle mass, to name a few. White participants had better performance than Black participants on the STS tests.

Conclusion

The generated centile curves reveal the pattern of change in muscle strength for tests recommended to identify probable sarcopenia. The curves can be used in rehabilitation to assess an individual’s performance relative to sex- and age-specific norms. To aid use of the data, a downloadable Excel-based calculator is provided to compute participant-specific percentiles, z scores, and t scores for each outcome and plot performance on the centile curves.

Impact

Physical therapists have an important role in identifying and treating individuals with sarcopenia and other causes of muscle weakness. The reference data provided for common clinical muscle strength tests provide therapists an ability to assess an individual’s relative performance.

Lay Summary

Knowing the normal or expected strength for an individual’s age and sex is essential to identifying muscle weakness. This study provides age- and sex-specific normal values for hand grip strength and sit-to-stand tests in adults aged 18 to 80 years.
Effects of Different Static Progressive Stretching Durations on Range of Motion, Myofibroblasts, and Collagen in a Posttraumatic Knee Contracture Rat Model
Authors: Wang Lu , Cui Jian-Bo , Xie Hui-Min , Zuo Xiu-Qin , He Jia-Le , Jia Zi-Shan , Zhang Li-Ning
Published: December 2021

Objective

The purpose of this study was to investigate the effects of different durations of static progressive stretching (SPS) on posttraumatic knee contracture in rats, including range of motion (ROM), gait analysis, myofibroblast proliferation, and collagen regulation.

Methods

The posttraumatic knee contracture model was established, and male Wistar rats were randomly divided into the 20-minute SPS treatment, 30-minute SPS treatment (S30), 40-minute SPS treatment, untreated, immobilization, and control groups. At Week 1, 2, and 4 of treatment intervention, joint ROM and gait were measured and compared. Knee joint samples stained with hematoxylin and eosin and Masson trichrome were used to observe alterations in pathological structures. Collagen density and cell numbers in the posterior joint capsule were used to assess joint capsule fibrosis and inflammation. Immunohistochemistry was used to detect type I collagen and α-smooth muscle actin expression.

Results

The S30 group improved the most; ROM, stance, mean intensity, print area, and stride length were 115 (SD = 5) degrees, 0.423 (SD = 0.074) seconds, 156.020 (SD = 7.952), 2.116 (SD = 0.078) cm2, and 11.758 (SD = 0.548) cm, respectively. The numbers of myofibroblasts, fibroblasts, and inflammatory cells decreased, and collagen proliferation was significantly suppressed in the S30 group compared with the other groups.

Conclusion

S30 significantly improved posttraumatic knee contracture in rats, with reduced type I collagen and α-smooth muscle actin expression, decreased the numbers of myofibroblasts and inflammatory cells, suppressed fibrotic and inflammatory changes in the joint capsule, and increased joint mobility. This study provided basic evidence for an optimal standard-of-care treatment approach for posttraumatic knee joint contracture in rats, which may have significance for humans.
Precarious Professionals: Layoffs and Gender Inequality in the Oil and Gas Industry
Authors: Bosky Amanda , Muller Chandra , Williams Christine L
Published: December 2021

As mass layoffs, downsizing, and corporate restructuring have become standard organizational practices, professional work is becoming less stable. We examine the effects of this turbulence on gender inequality through a case study of layoffs in the oil and gas industry. Using a longitudinal survey that followed a cohort of recently hired scientists and engineers at a multinational oil and gas company from 2012–2016, we find that a major reduction-in-force during our study resulted in significant gender disparities in firm departures. Results indicate that about seventy percent of the gender gap in departures can be explained by underlying gender differences in the formal layoff criteria and workers’ experiences in the firm prior to layoffs, pointing to formal organizational processes as insidious mechanisms driving inequality. The firm stated that layoffs were determined by performance and skill set, but the actual attributes for surviving layoffs turned out to be more malleable and open to bias. Results suggest that the firm’s performance rating system may provide a pathway through which women’s relational disadvantages became formalized in seemingly bureaucratically neutral ways. Our findings illustrate how increasing insecurity in professional careers may disadvantage women and shed light on the organizational mechanisms that reproduce gender inequality in professional work today.
Metaplastic breast cancers and triple-negative breast cancers of no special type: are they prognostically different? A systematic review and meta-analysis
Authors: Corso Giovanni , D’Ecclesiis Oriana , Magnoni Francesca , Mazzotta Erica , Conforti Fabio , Veronesi Paolo , Sajjadi Elham , Venetis Konstantinos , Fusco Nicola , Gandini Sara
Published: December 2021

Background and aims

Metaplastic breast cancer (MBC) and triple-negative (TN) BC of no special type are often confounded with each other in terms of survival and prognosis. In this systematic study and meta-analysis, we evaluated the prognosis of each of these two different diagnoses.

Methods

We conducted a systematic literature search and review using the MOOSE guidelines, through PUBMED database, the Ovid MEDLINE database, and the ISI Web of Science Citation Index Expanded (SCI Expanded). Overall survival (OS) and disease-free survival (DFS) were the main outcomes considered.

Results

Our review eventually selected six independent studies, with a total of more than 59 519 patients. MBC was found to associate with worse OS compared to TNBC of no special type, with a significant 40% increased risk of death [summary hazard ratio (SHR) = 1.40, 95% confidence interval (CI): 1.30–1.50]. We found neither heterogeneity (I
2 = 0%) nor evidence of publication bias (P = 0.82 and P = 0.49 by Begg’s and Egger’s test, respectively) between studies. No statistically significant difference was found between MBC and TNBC of no special type in terms of DFS (SHR = 1.17, 95% CI: 0.80–1.71).

Conclusion

This study demonstrates that TNBC of no special type and MBC have comparable DFS, although the latter presents a significantly worse prognosis in terms of OS. Despite DFS being similar in both subtypes, this did not result in significant OS benefits, with MBC score being the worse of the two diseases.
Bidirectional associations between cognitive functions and walking performance among middle-aged women
Authors: Tirkkonen Anna , Kekäläinen Tiia , Aukee Pauliina , Kujala Urho M. , Laakkonen Eija K. , Kokko Katja , Sipilä Sarianna
Published: December 2021

Objective:

This study investigated whether (1) cognitive functions change after the transition from the perimenopausal to the postmenopausal stage, (2) cognitive functions and walking are associated in middle-aged women, and (3) cognitive functions assessed in perimenopause are associated with walking after reaching the postmenopause or vice versa.

Methods:

In total, 342 women, categorized as early (n = 158) or late perimenopausal (n = 184), were included in the study and followed up until postmenopausal. Psychomotor speed, executive functions related to set-shifting and updating, working memory, and visual memory were assessed. Walking was assessed with walking speed, walking distance, and dual-task cost in walking speed. Data was analyzed using the paired-samples t test, Wilcoxon signed rank test, multiple linear regression analysis, and structural equation modeling.

Results:

We found small but significant improvements in psychomotor speed (P = 0.01) and working memory (P < 0.001) among early perimenopausal and in psychomotor speed (P = 0.001), set-shifting (P = 0.02), visual memory (P = 0.002), and working memory (P < 0.001) among late perimenopausal women after the transition from peri- to postmenopause. Walking speed (β = 0.264, P = 0.001) and dual-task cost (β = 0.160, P = 0.03) were associated with updating, and walking distance was associated with updating and set-shifting (β = 0.198, P = 0.02, β=−0.178 P = 0.04 respectively) among the late perimenopausal women. We found no longitudinal associations between cognitive functions and walking.

Conclusion:

Cognitive performance remained unchanged or improved after reaching postmenopause. Cognitive functions and walking were associated during the late perimenopause, but the association depended on the cognitive process and nature of the physical task. Cognitive performance was not associated with walking after reaching postmenopause or vice versa.
Anesthesia Considerations for Pregnant People With COVID-19 Infection
Authors: Landau Ruth , Bernstein Kyra , Ring Laurence E.
Published: December 2021

The purpose of this review is to describe updates following initial recommendations on best anesthesia practices for obstetric patients with coronavirus disease 2019. The first surge in the United States prompted anesthesiologists to adapt workflows and reconsider obstetric anesthesia care, with emphasis on avoidance of general anesthesia, the benefit of early neuraxial labor analgesia, and prevention of emergent cesarean delivery whenever possible. While workflows have changed to allow sustained safety for obstetric patients and health care workers, it is notable that obstetric anesthesia protocols for labor and delivery have not significantly evolved since the first coronavirus disease 2019 wave.
The Role of Artificial Intelligence in the Diagnosis and Management of Glaucoma
Authors: Ittoop Sabita M. , Jaccard Nicolas , Lanouette Gabriella , Kahook Malik Y.
Published: December 2021

Glaucomatous optic neuropathy is the leading cause of irreversible blindness worldwide. Diagnosis and monitoring of disease involves integrating information from the clinical examination with subjective data from visual field testing and objective biometric data that includes pachymetry, corneal hysteresis, and optic nerve and retinal imaging. This intricate process is further complicated by the lack of clear definitions for the presence and progression of glaucomatous optic neuropathy, which makes it vulnerable to clinician interpretation error. Artificial intelligence (AI) and AI-enabled workflows have been proposed as a plausible solution. Applications derived from this field of computer science can improve the quality and robustness of insights obtained from clinical data that can enhance the clinician’s approach to patient care. This review clarifies key terms and concepts used in AI literature, discusses the current advances of AI in glaucoma, elucidates the clinical advantages and challenges to implementing this technology, and highlights potential future applications.
Examination of social disconnectedness and perceived social isolation on health and life satisfaction in community-dwelling adults with dysvascular lower limb loss
Authors: Hitzig Sander L. , Dilkas Steven , Payne Michael W. , MacKay Crystal , Viana Ricardo , Devlin Michael , Cimino Stephanie R. , Guilcher Sara J.T. , Mayo Amanda L.
Published: December 2021

Background:

People with physical disabilities are at risk for social isolation, which has been shown to negatively influence health and well-being.

Objective(s):

The purpose of this study was to examine the association of social disconnectedness and perceived social isolation (PSI) on health-related quality of life (HrQoL) and life satisfaction in adults with dysvascular lower extremity amputation (LEA).

Study Design:

Cross-sectional survey.

Methods:

Data were collected from community-dwelling adults with dysvascular major LEA (N = 231). The main outcome measures were the Short-Form 36 and the Life Satisfaction-11. Other measures were used to quantify comorbidities/secondary health conditions, PSI, social disconnectedness, self-efficacy, social support, and social engagement. Three hierarchical regression models were conducted to predict (1) physical HrQoL, (2) mental HrQoL, and (3) life satisfaction.

Results:

Approximately one-third of the sample had high levels of social disconnectedness and PSI. The regression model predicting physical HrQoL accounted for 47% of the variance and only found that higher levels of mobility, lower perceived impact of comorbidities/conditions, and higher levels of social engagement and self-efficacy were associated with better physical HrQoL. A model predicting mental HrQoL accounted for 36% of the variance, with older age, lower PSI, and higher levels of self-efficacy being significant predictors. Finally, the model predicting life satisfaction accounted for 56% of the variance, with older age, lower perceived impact of comorbidities/conditions, and higher self-efficacy remaining as significant predictors.

Conclusions:

These findings highlight that one-third of our sample were at risk for being socially isolated and that PSI was influential on mental HrQoL and life satisfaction. There is a need for approaches to address social isolation for people with dysvascular LEA living in the community to optimize their long-term health and well-being.
The incidence and trend of geriatric amputations in Taiwan from 1996 to 2013
Authors: Huang Ya-Ling , Ho Wei-Te , Wu Chun-Chieh , Li Tung-Chou
Published: December 2021

Background:

There has been a reported reduction in the incidence of amputation, but it is unclear whether the number of amputations has decreased in the elderly, a cohort that typically has the largest proportion of amputees.

Objectives:

To investigate the incidence proportion and time trends of amputation in patients aged ≥ 65 years in Taiwan.

Study design:

A retrospective cohort study from a large national database.

Methods:

The records of patients who underwent an amputation were collected from a nationally representative sample of 1,000,000 enrollees of Taiwan's National Health Insurance program during 1996–2013. The patients were divided into four age groups: ≤64, 65–74, 75–84, and ≥85 years. Joinpoint regression was performed with adjustment for age and sex to identify changes in incidence proportion by year.

Results:

During the 18 years, the incidence of upper and lower limb amputation decreased significantly in the total population, with the average annual percentage change (AAPC) of −6.1 and −1.8, respectively. However, in the elderly population over 65 years, the incidence did not decrease significantly for upper minor amputation, lower minor amputation, and major amputation with the AAPC of −1.1, −0.1, and −0.4, respectively. Although not significant, the incidence of major and minor lower limb amputation in the population over 85 years old showed an increasing trend, with the AAPC of 1.2 and 3.2, respectively.

Conclusion:

During the study period, although the incidence of amputation of the overall population decreased in Taiwan, this trend was not simultaneously observed in the elderly and hence, it should not be ignored.
Prosthetic walking after bilateral transfemoral amputation in a patient with dilated cardiomyopathy: a case report
Authors: Kanata Yoshihiro , Ito Tomoyuki , Yoshida Takuya , Koyama Tetsuo , Mikami Yasuo , Domen Kazuhiza
Published: December 2021

In this study, we present a case of a 14-year-old adolescent boy who developed rhabdomyolysis and underwent bilateral transfemoral amputation after cardiopulmonary resuscitation for cardiac arrest because of dilated cardiomyopathy (DCM).

Patients with bilateral transfemoral amputation have slower walking velocity and greater oxygen consumption during walking. Rehabilitation of such patients may be demanding especially if they have DCM, one of the major causes of advanced heart failure.

The patient was admitted to our hospital on postoperative day (POD) 206. At first, we started with residuum compression. Simultaneously, resistance training and range-of-motion exercise were performed. He started the walking training with short stubbies prostheses on POD 262. Then, we used two types of knee joint prostheses, microprocessor-controlled prosthetic knee (MPK) and non-MPK. We chose MPK for his bilateral knee joints because it was easier for him to walk with MPK than with non-MPK. MPK also has the advantage of high stability and less burden on the heart.

He achieved to walk with the prosthesis using T-canes with both hands on POD 374. Furthermore, he was able to return to school after discharge.

In patients with transfemoral amputations and DCM with exercise restrictions, MPK is preferred because it reduces cardiac load during physical activity.
The Effect of Bone Density Measured by Cone Beam Computed Tomography and Implant Dimensions on the Stability of Dental Implants
Authors: Noaman Ali Tareef , Bede Salwan Y.
Published: December 2021

The aim of this study was to evaluate the effect of bone density value in Hounsfield unit derived from cone beam computed tomography (CBCT), and implant dimensions in relation to implant stability parameters namely the resonance frequency analysis and the insertion torque (IT) value. It included 24 patients who received 42 dental implants (DI). The bone density of the planned implant site was preoperatively measured using cone beam computed tomography. The implant stability was measured using Osstell implant stability quotient (ISQ). The ISQ values were recorded immediately postoperatively and after 16 weeks. The IT value was categorized as 35 N/cm or > 35 N/cm. The mean (standard deviation) primary stability was 79.58 (5.27) ISQ, which was significantly higher than the secondary stability 74.31 (6.34) ISQ (P < 0.0001). There was a significant moderate positive correlation of bone density with primary stability (r = 0.4, P = 0.0099) and no correlation with secondary stability (r = 0.003, P = 0.9867). The bone density of DI with 35 N/cm IT was significantly lower than with > 35 N/cm IT (P = 0.0390). Better stability was recorded with wider implants. Whereas the length of the DI showed a nonsignificant correlation with primary and secondary stability (P = 0.7633 and 0.4670, respectively). The DI dimensions showed a nonsignificant correlation with the IT. Cone beam computed tomography may be considered as a reliable method to assess bone density and predict the implant stability. The diameter of DI affected the implant stability favorably, whereas DI length showed no effect.
ACE2-independent SARS-CoV-2 infection and mouse adaption emerge after passage in cells expressing human and mouse ACE2
Authors: Yan Kexin , Dumenil Troy , Tang Bing , Le Thuy T. , Bishop Cameron , Suhrbier Andreas , Rawle Daniel J.
Published: December 2021

Human ACE2 (hACE2) is the key cell attachment and entry receptor for SARS-CoV-2, with the original SARS-CoV-2 isolates unable to use mouse ACE2 (mACE2). Herein we describe a new system for generating mouse-adapted SARS-CoV-2 in vitro by serial passaging virus in co-cultures of cell lines expressing hACE2 and mACE2. Mouse-adapted viruses emerged with up to five amino acid changes in the spike protein, all of which have been seen in human isolates. Mouse-adapted viruses replicated to high titers in C57BL/6J mouse lungs and nasal turbinates, and caused severe lung histopathology. One mouse-adapted virus was also able to replicate efficiently in ACE2-negative cell lines, with ACE2-independent entry by SARS-CoV-2 representing a new biology for SARS-CoV-2 that has potential widespread implications for disease and intervention development.
Spatial and temporal requirement of Mlp60A isoforms during muscle development and function in Drosophila melanogaster
Authors: Wishard Rohan , Jayaram Mohan , Ramesh S. R. , Nongthomba Upendra
Published: December 2021

Many myofibrillar proteins undergo isoform switching in a spatio-temporal manner during muscle development. The biological significance of the variants of several of these myofibrillar proteins remains elusive. One such myofibrillar protein, the Muscle LIM Protein (MLP), is a vital component of the Z-discs. In this paper, we show that one of the Drosophila MLP encoding genes, Mlp60A, gives rise to two isoforms: a short (279 bp, 10 kDa) and a long (1461 bp, 54 kDa) one. The short isoform is expressed throughout development, but the long isoform is adult-specific, being the dominant of the two isoforms in the indirect flight muscles (IFMs). A concomitant, muscle-specific knockdown of both isoforms leads to late pupal lethality, with the surviving flies being majorly flight defective. Mlp60A null flies show developmental lethality, and muscle defects in the individuals surviving till the third instar larval stage. This lethality could be rescued partially by muscle-specific overexpression of the short isoform. Almost 90% of the long isoform-specific P-element insertion mutant flies show a compromised flight ability and have reduced sarcomere length. Hence, our data shows that the two Mlp60A isoforms are functionally specialized, to ensuring normal embryonic muscle development and adult flight muscle function.
Neutrophil Profiles of Pediatric COVID-19 and Multisystem Inflammatory Syndrome in Children
Authors: Boribong Brittany P. , LaSalle Thomas J. , Bartsch Yannic C. , Ellett Felix , Loiselle Maggie E. , Davis Jameson P. , Gonye Anna L. K. , Hajizadeh Soroush , Kreuzer Johannes , Pillai Shiv , Haas Wilhelm , Edlow Andrea , Fasano Alessio , Alter Galit , Irimia Daniel , Sade-Feldman Moshe , Yonker Lael M.
Published: December 2021

Multisystem Inflammatory Syndrome in Children (MIS-C) is a delayed-onset, COVID-19-related hyperinflammatory systemic illness characterized by SARS-CoV-2 antigenemia, cytokine storm and immune dysregulation; however, the role of the neutrophil has yet to be defined. In adults with severe COVID-19, neutrophil activation has been shown to be central to overactive inflammatory responses and complications. Thus, we sought to define neutrophil activation in children with MIS-C and acute COVID-19. We collected samples from 141 children: 31 cases of MIS-C, 43 cases of acute pediatric COVID-19, and 67 pediatric controls. We found that MIS-C neutrophils display a granulocytic myeloid-derived suppressor cell (G-MDSC) signature with highly altered metabolism, which is markedly different than the neutrophil interferon-stimulated gene (ISG) response observed in pediatric patients during acute SARS-CoV-2 infection. Moreover, we identified signatures of neutrophil activation and degranulation with high levels of spontaneous neutrophil extracellular trap (NET) formation in neutrophils isolated from fresh whole blood of MIS-C patients. Mechanistically, we determined that SARS-CoV-2 immune complexes are sufficient to trigger NETosis. Overall, our findings suggest that the hyperinflammatory presentation of MIS-C could be mechanistically linked to persistent SARS-CoV-2 antigenemia through uncontrolled neutrophil activation and NET release in the vasculature.

One Sentence Summary

Circulating SARS-CoV-2 antigen:antibody immune complexes in Multisystem Inflammatory Syndrome in Children (MIS-C) drive hyperinflammatory and coagulopathic neutrophil extracellular trap (NET) formation and neutrophil activation pathways, providing insight into disease pathology and establishing a divergence from neutrophil signaling seen in acute pediatric COVID-19.
Socialization of Providencia stuartii enables resistance to environmental insults
Authors: Lopes Julie , Tetreau Guillaume , Pounot Kevin , El Khatib Mariam , Colletier Jacques-Philippe
Published: December 2021

Providencia stuartii is a highly-social pathogen responsible for nosocomial chronic urinary tract infections. The bacterium indeed forms floating communities of cells (FCC) besides and prior-to canonical surface-attached biofilms (SAB). Within P. stuartii FCC, cells are riveted one to another owing to by self-interactions between its porins, viz. Omp-Pst1 and Omp-Pst2. In pathophysiological conditions, P. stuartii is principally exposed to high concentrations of urea, ammonia, bicarbonate, creatinine and to large variations of pH, questioning how these environmental cues affect socialization, and whether formation of SAB and FCC protects cells against those. Results from our investigations indicate that FCC and SAB can both form in the urinary tract, endowing cells with increased resistance and fitness. They additionally show that while Omp-Pst1 is the main gateway allowing penetration of urea, bicarbonate and ammonia into the periplasm, expression of Omp-Pst2 enables resistance to them.
Re-evaluation of the phylogenetic diversity and global distribution of the genusCandidatusAccumulibacter
Authors: Petriglieri Francesca , Singleton Caitlin , Kondrotaite Zivile , Dueholm Morten K. D. , McDaniel Elizabeth A. , McMahon Katherine D. , Nielsen Per H.
Published: December 2021

CandidatusAccumulibacter was the first microorganism identified as a polyphosphate-accumulating organism (PAO), important for phosphorus removal from wastewater. This genus is diverse, and the current phylogeny and taxonomic framework appears complicated, with the majority of publicly available genomes classified as “CandidatusAccumulibacter phosphatis”, despite notable phylogenetic divergence. Theppk1marker gene allows for a finer scale differentiation into different “types” and “clades”, nevertheless taxonomic assignments remain confusing and inconsistent across studies. Therefore, a comprehensive re-evaluation is needed to establish a common understanding of this genus, both in terms of naming and basic conserved physiological traits. Here, we provide this re-assessment using a comparison of genome,ppk1, and 16S rRNA gene-based approaches from comprehensive datasets. We identified 15 novel species, along with the well-knownCa. A. phosphatis,Ca. A. deltensis andCa. A. aalborgensis. To compare the speciesin situ, we designed new species-specific FISH probes and revealed their morphology and arrangement in activated sludge. Based on the MiDAS global survey,Ca. Accumulibacter species were widespread in WWTPs with phosphorus removal, indicating the process design as a major driver for their abundance. Genome mining for PAO related pathways and FISH-Raman microspectroscopy confirmed the potential for the PAO metabolism in allCa. Accumulibacter species, with detectionin situof the typical PAO storage polymers. Genome annotation further revealed fine-scale differences in the nitrate/nitrite reduction pathways. This provides insights into the niche differentiation of these lineages, potentially explaining their coexistence in the same ecosystem while contributing to overall phosphorus and nitrogen removal.

Importance

CandidatusAccumulibacter is the most studied PAO organism, with a primary role in biological nutrient removal. However, the species-level taxonomy of this lineage is convoluted due to the use of different phylogenetic markers or genome sequencing. Here, we redefined the phylogeny of these organisms, proposing a comprehensive approach which could be used to address the classification of other diverse and uncultivated lineages. Using genome-resolved phylogeny, compared to 16S rRNA gene- and other phylogenetic markers phylogeny, we obtained a higher resolution taxonomy and established a common understanding of this genus. Furthermore, genome mining of gene and pathways of interest, validatedin situby application of a new set of FISH probes and Raman micromicrospectroscopy, provided additional high-resolution metabolic insights into these organisms.
Single-cell Senseless protein analysis reveals metastable states during the transition to a sensory organ fate
Authors: Giri Ritika , Brady Shannon , Papadopoulos Dimitrios K. , Carthew Richard W.
Published: December 2021

Cell fate decisions can be envisioned as bifurcating dynamical systems, and the decision that Drosophila cells make during sensory organ differentiation has been described as such. We extended these studies by focusing on the Senseless protein, which orchestrates sensory cell fate transitions. Wing cells contain intermediate Senseless numbers prior to their fate transition, after which they express much greater numbers of Senseless molecules as they differentiate. However, the dynamics are inconsistent with it being a simple bistable system. Cells with intermediate Senseless are best modeled as residing in four discrete states, each with a distinct protein number and occupying a specific region of the tissue. Although the states are stable over time, the number of molecules in each state vary with time. The fold-change in molecule number between adjacent states is invariant and robust to absolute protein number variation. Thus, cells transitioning to sensory fates exhibit metastability with relativistic properties.
A Modified TurboID Approach Identifies Tissue-Specific Centriolar Components InC. elegans
Authors: Holzer Elisabeth , Rumpf-Kienzl Cornelia , Falk Sebastian , Dammermann Alexander
Published: December 2021

Proximity-dependent labeling approaches such as BioID have been a great boon to studies of protein-protein interactions in the context of cytoskeletal structures such as centrosomes which are poorly amenable to traditional biochemical approaches like immunoprecipitation and tandem affinity purification. Yet, these methods have so far not been applied extensively to invertebrate experimental models such asC. elegansgiven the long labeling times required for the original promiscuous biotin ligase variant BirA*. Here, we show that the recently developed variant TurboID successfully probes the interactomes of both stably associated (SPD-5) and dynamically localized (PLK-1) centrosomal components. We further develop an indirect proximity labeling method employing a GFP nanobody-TurboID fusion, which allows the identification of protein interactors in a tissue-specific manner in the context of the whole animal. Critically, this approach utilizes available endogenous GFP fusions, avoiding the need to generate multiple additional strains for each target protein and the potential complications associated with overexpressing the protein from transgenes. Using this method, we identify homologs of two highly conserved centriolar components, Cep97 and Bld10/Cep135, which are present in various somatic tissues of the worm. Surprisingly, neither protein is expressed in early embryos, likely explaining why these proteins have escaped attention until now. Our work expands the experimental repertoire forC. elegansand opens the door for further studies of tissue-specific variation in centrosome architecture.
PlantRNA 2.0: an updated database dedicated to tRNAs of photosynthetic eukaryotes
Authors: Cognat Valérie , Pawlak Gael , Pflieger David , Drouard Laurence
Published: December 2021

PlantRNA (http://plantrna.ibmp.cnrs.fr/) is a comprehensive database of transfer RNA (tRNA) gene sequences retrieved from fully annotated nuclear, plastidial and mitochondrial genomes of photosynthetic organisms. In the first release (PlantRNA 1.0), tRNA genes from 11 organisms were annotated. In this second version, the annotation was implemented to 51 photosynthetic species covering the whole phylogenetic tree of photosynthetic organisms, from the most basal group of Archeplastida, the glaucophyte Cyanophora paradoxa, to various land plants. Transfer RNA genes from lower photosynthetic organisms such as streptophyte algae or lycophytes as well as extremophile photosynthetic species such as Eutrema parvulum were incorporated in the database. As a whole, circa 37 000 tRNA genes were accurately annotated. In the frame of the tRNA genes annotation from the genome of the Rhodophyte Chondrus crispus, non-canonical splicing sites in the D- or T- regions of tRNA molecules were identified and experimentally validated. As for PlantRNA 1.0, comprehensive biological information including 5’- and 3’-flanking sequences, A and B box sequences, region of transcription initiation and poly(T) transcription termination stretches, tRNA intron sequences and tRNA mitochondrial import are included.
Opportunities and challenges of applying advanced X-ray spectroscopy to actinide and lanthanide N-donor ligand systems
Authors: Pruessmann Tim , Nagel Peter , Simonelli Laura , Batchelor David , Gordon Robert , Schimmelpfennig Bernd , Trumm Michael , Vitova Tonya
Published: December 2021

N-donor ligands such as n-Pr-BTP [2,6-bis(5,6-dipropyl-1,2,4-triazin-3-yl)pyridine] preferentially bind trivalent actinides (An3+) over trivalent lanthanides (Ln3+) in liquid–liquid separation. However, the chemical and physical processes responsible for this selectivity are not yet well understood. Here, an explorative comparative X-ray spectroscopy and computational (L
3-edge) study for the An/Ln L
3-edge and the N K-edge of [An/Ln(n-Pr-BTP)3](NO3)3, [Ln(n-Pr-BTP)3](CF3SO3)3 and [Ln(n-Pr-BTP)3](ClO4)3 complexes is presented. High-resolution X-ray absorption near-edge structure (HR-XANES) L
3-edge data reveal additional features in the pre- and post-edge range of the spectra that are investigated using the quantum chemical codes FEFF and FDMNES. X-ray Raman spectroscopy studies demonstrate the applicability of this novel technique for investigations of liquid samples of partitioning systems at the N K-edge.
Extending the boundaries of psychological ownership research: measurement, outcomes, cultural moderators
Authors: Renz Franziska M. , Posthuma Richard , Smith Eric
Published: December 2021

Purpose

Psychological ownership (PO) theory and extended self theory explain why someone feels like the owner of his/her job or organization. Yet, there is limited prior research examining whether PO differs as an individual versus collective phenomenon, and in different cultural contexts. The authors extend this literature by examining the dimensionality of PO, multiple outcomes and cultural values as boundary conditions.

Design/methodology/approach

Data from surveys of 331 supervisors from Mexico and the US were collected to examine the relationships between the theorized constructs. The authors apply two-stage least squares (2SLS) regression analysis to alleviate endogeneity concerns and produce robust results.

Findings

Both individual and collective PO (IPO and CPO) are positively associated with organization-based self-esteem (OBSE) and a new outcome, paternalistic leadership behavior. Cultural values are significant moderators with an individualistic orientation enhancing and a power distance orientation attenuating these relationships.

Originality/value

This study extends PO theory and extended self theory by investigating whether IPO and CPO have different outcomes considering contextual differences in cultural values. Additionally, the authors capture the frequency of paternalism instead of its mere occurrence.
The nasty face of the liability of foreignness: MNCs and rent extraction
Authors: Kozan Asli
Published: December 2021

Purpose

This study aims to clarify the factors that act as a buffer to rent extraction from multi-national corporations (MNCs) in exchange relationships with the host country’s political actors.

Design/methodology/approach

This study proposes a conceptual model of the factors that determine rent extraction by host country political actors from MNCs. The model identifies the sources of power the MNC can use to alleviate the power imbalance relative to the political actor to decrease rent extraction. Additionally, it identifies the factors that constrain the power-advantaged political actor, thus moderating the relationship between power imbalance and rent extraction.

Findings

This conceptual paper’s propositions remain for future empirical validation.

Originality/value

This study integrates insights from the international business literature and resource dependence theory (RDT) to identify the determinants of firm-specific rent extraction risk for MNCs. First, the model sheds light on the heterogeneity among MNCs in their susceptibility to rent extraction and their ability to manage their liability of foreignness in the host country. Second, by integrating the horizontal and vertical distribution of power in the political environment to analyze the power-dependence relationship between the MNC and host country political actors, the framework addresses a shortcoming of RDT and accounts for the dynamics of the external environment for MNCs managing their dependencies. This study also provides a basis for discussing the rent extraction MNCs face worldwide and lays the foundation for future empirical works.
Migrant’s library services: a theoretical and empirical study in Spain with guidelines for their creation
Authors: Serrano Pérez Luis , Faba-Pérez Cristina
Published: December 2021

Purpose

The purpose of this research study was to seek new forms of public libraries helping with the integration of migrants. It comprises a comprehensive and up-to-date bibliographic review and an empirical study involving the librarians and native and migrant users of public libraries in Spain leading to the design of guidelines for library services targeted at migrant users that can help foster the integration of this group of the population.

Design/methodology/approach

The bibliographic review analyses studies of whether migrant users see the public library as a place of meeting and interchange as well as a fundamental institution providing them with services, meeting their information requirements and offering them challenges and projects. The empirical investigation contrasts this theoretical information by administering three questionnaires (one for librarians, one for native users and one for migrant users, with a total of 45 items) focused on the relationship between public libraries and migration. A total of 20 public libraries over the territory of Spain were selected in accordance with diverse criteria, and a total of 233 completed questionnaires were collected.

Findings

The main results of the empirical research indicate that the greatest proportion of migrant users go to Spain’s public libraries on the recommendation of friends and/or relatives. They use them mainly as a place of study, visiting them at least twice a week, even though the representation of collections and specific services for this group is very sparse (only 5% of the collection of the libraries analysed are in a foreign language). On the other hand, the satisfaction expressed towards both the general services of the libraries and the help provided by their librarians is scored highly, getting mean scores of 4.45 and 4.40 out of 5, respectively.

Originality/value

This work not only includes an exhaustive bibliographic review of the relationship between public libraries and migrant users and an empirical investigation carried out in Spain with librarians, native users and migrant users, but also presents a decalogue of guidelines for the design of a range of services focused on the specific needs of the migrant population, thus favouring their integration in the host country.
Contact fatigue life prediction of rolling bearing considering machined surface integrity
Authors: Cui Li , Su Yin
Published: December 2021

Purpose

Rolling bearings often cause engineering accidents due to early fatigue failure. The study of early fatigue failure mechanism and fatigue life prediction does not consider the integrity of the bearing surface. The purpose of this paper is to find new rolling contact fatigue (RCF) life model of rolling bearing.

Design/methodology/approach

An elastic-plastic finite element (FE) fatigue damage accumulation model based on continuous damage mechanics is established. Surface roughness, surface residual stress and surface hardness of bearing rollers are considered. The fatigue damage and cumulative plastic strain during RCF process are obtained. Mechanism of early fatigue failure of the bearing is studied. RCF life of the bearing under different surface roughness, hardness and residual stress is predicted.

Findings

To obtain a more accurate calculation result of bearing fatigue life, the bearing surface integrity parameters should be considered and the elastic-plastic FE fatigue damage accumulation model should be used. There exist the optimal surface parameters corresponding to the maximum RCF life.

Originality/value

The elastic-plastic FE fatigue damage accumulation model can be used to obtain the optimized surface integrity parameters in the design stage of bearing and is helpful for promote the development of RCF theory of rolling bearing.
Representation and the problem of bibliographic imagination on Wikipedia
Authors: Luyt Brendan
Published: December 2021

Purpose

The astonishing thing about Wikipedia is that despite the way it is produced, the product is as good as it is and not far worse. But this is no reason for complacency. As others have documented, Wikipedia has representational blind spots, produced by the nature of its editorial community and their discursive conventions. This article wishes to look at the potential effect of sources on certain of these blind spots.

Design/methodology/approach

The author used an extended example, the Wikipedia article on the Philippine–American War, to illustrate the unfortunate effects that accompany a lack of attention to the kind of sources used to produce narratives for the online encyclopaedia. The Philippine–American War article was chosen because of its importance to American history. The war brought to the fore a debate over the future of the USA and the legitimacy of a republic acquiring overseas colonies. It remains controversial today, making it essential that its representation on Wikipedia is soundly constructed.

Findings

Inattention to sources (a lack of bibliographical imagination) produces representational anomalies. Certain sources are privileged when they should not be and others are ignored or considered as sub-standard. Overall, the epistemological boundaries of the article in terms of what the editorial community considers reliable and what the community of scholars producing knowledge about the war think as reliable do not overlap to the extent that they should. The resulting narrative is therefore less rich than it otherwise could be.

Originality/value

While there exists a growing literature on the representational blind spots of Wikipedia (gender, class, geographical region and so on), the focus has been on the composition of the demographics of the editorial community. But equally important to the problem of representation are the sources used by that community. Much literature has been written that seeks to portray the social world of the marginalized, but it is not used on Wikipedia, despite it easily meeting the criteria for reliability set by the Wikipedia community. This is a tragic oversight that makes Wikipedia's aim to be a repository for the knowledge of the world, a laudable goal to strive for, even if in reality unobtainable, even harder to achieve than ever.
The quality continuum: perceptions of institutional accreditation
Authors: Hoare Alana , Goad Pamela
Published: December 2021

Purpose

Academics and administrators frequently lament the bureaucracy in higher education, which diverts attention from teaching and research. Heightened monitoring of institutions as a result of dominant neoliberal ideologies shapes perceptions of accreditation as a bureaucratic burden rather than a value-added tool for continuous quality improvement. This paper aims to identify factors that positively and negatively impact a culture of quality in North American accredited postsecondary institutions to address issues of equity and inform policy changes that are relevant to community needs.

Design/methodology/approach

Academics’ and administrators’ perceptions of accreditation processes are explored through an interpretivist mixed-methods study that combined focus group and survey results from over 200 participants representative of four-year private and four- and two-year public institutions and tribal colleges in North America.

Findings

Findings suggest that a utilitarian and exclusionary mindset perpetuated by neoliberal logics restricts participatory decision-making processes in postsecondary institutions. Furthermore, the research identified a noticeable gap between those who are invited to participate in accreditation processes and those who contribute to decision-making. This lack of inclusive governance inhibits the ability of institutions to respond appropriately to the needs of its community.

Originality/value

The scope of the study and prioritization of qualitative data offers a comprehensive picture of academics’ and administrators’ perceptions and positions them as the experts in their own learning and development. Through participant narratives, strategies for increasing the value of quality assurance processes are illuminated. As a result, the study participants become the change agents who provide the solutions for ameliorating academics’ and administrators’ resistance to quality assurance.