bluuebunny/crossref_metadata_2025 huggingface dataset:
Differences in Characteristics of Photosynthesis and Nitrogen Utilization in Leaves of the Black Locust (Robinia pseudoacacia L.) According to Leaf Position
Authors: Choi Dongsu , Jang Woongsoon , Toda Hiroto , Yoshikawa Masato
Published: March 2021

Robinia pseudoacacia L. has been widely planted worldwide for a variety of purposes, but it is a nonindigenous species currently invading the central part of Japanese river terraces. To understand and control this invasion, we investigated how this species invests nitrogen resources in different functions depending on the leaf location, and how these resources are used in physiological reactions such as photosynthesis. The Tama river terrace was examined in Tokyo, Japan. The leaf nitrogen (N) concentration, chlorophyll (Chl) concentration, Chl a/b ratio, leaf mass per unit area (LMA) and ribulose-1,5-bisphosphate carboxylase oxygenase (RuBisCo) concentration were all significantly lower in shade leaves than in leaves exposed to the sun. Conversely, the net photosynthetic rate in saturated light conditions (Pmax), the net photosynthetic rate under enhanced CO2 concentration and light saturation (Amax), the maximum carboxylation rate of RuBisCo (Vcmax) and the maximum rate of electron transport driving RUBP regeneration (Jmax) were all significantly lower in shade leaves than in leaves exposed to the sun. We also found that RuBisCo/N and Chl/N were significantly less in shade leaves, and values of Jmax/N, Vcmax/N less in shade leaves than in sun leaves, but not significantly. Allocation of nitrogen in leaves to photosynthetic proteins, RuBisCo (NR) was broadly less in shade leaves, and NL (light-harvesting complex: LHC, photosystem I and II: PSI and PSII) and NE (electron transport) were also lower. The N remaining was much greater in shade leaves than in sun leaves. We suggest that N remobilization from RuBisCo is more efficient than remobilization from proteins of NE, and from NL. This study shows that R. pseudoacacia has an enhanced ability to adapt to environmental changes via characteristic changes in N allocation trade-offs and physiological traits in its sun and shade leaves.
Characterization of the Digital Identity of Chilean University Students Considering Their Personal Learning Environments
Authors: Hernández-Orellana Marisol , Pérez-Garcias Adolfina , Roco-Videla Ángel
Published: March 2021

At present, our online activity is almost constant, either producing information or consuming it, both for the social and academic fields. The spaces in which people move and travel every day, innocently divided between the face-to-face and the virtual, affect the way we communicate and perceive ourselves. In this document, a characterization of the academic digital identity of Chilean university students is proposed and an invitation to teachers to redefine learning spaces is made, allowing integrating all those technological tools that the student actually uses. This study was developed within the logic of pragmatism based on mixed methodology, non-experimental design, and a descriptive–quantitative cross-sectional approach. A non-probabilistic sample was made up of 509 students, who participated voluntarily with an online questionnaire. The Stata Version-14 program was used, applying the Mann–Whitney–Wilcoxon and Kruskal–Wallis U tests. To develop characterizations, a conglomerate analysis was performed with a hierarchical dissociative method. In general, Chilean university students are highly truthful on the Internet without making significant differences between face-to-face and digital interactions, with low awareness of their ID, being easily recognizable on the Web. Regarding their educational process, they manage it with analogical/face-to-face mixing formal and informal technological tools to optimize their learning process. These students manifest a hybrid academic digital identity, without gender difference in the deployment of their PLEs, but maintaining stereotypical gender behaviors in the construction of their digital identity on the Web, which shows a human-technological development similar to that of young Asians and Europeans.
Dirichlet Process Prior for Student’s t Graph Variational Autoencoders
Authors: Zhao Yuexuan , Huang Jing
Published: March 2021

Graph variational auto-encoder (GVAE) is a model that combines neural networks and Bayes methods, capable of deeper exploring the influential latent features of graph reconstruction. However, several pieces of research based on GVAE employ a plain prior distribution for latent variables, for instance, standard normal distribution (N(0,1)). Although this kind of simple distribution has the advantage of convenient calculation, it will also make latent variables contain relatively little helpful information. The lack of adequate expression of nodes will inevitably affect the process of generating graphs, which will eventually lead to the discovery of only external relations and the neglect of some complex internal correlations. In this paper, we present a novel prior distribution for GVAE, called Dirichlet process (DP) construction for Student’s t (St) distribution. The DP allows the latent variables to adapt their complexity during learning and then cooperates with heavy-tailed St distribution to approach sufficient node representation. Experimental results show that this method can achieve a relatively better performance against the baselines.
Transcriptomic and Metabolomic Basis of Short- and Long-Term Post-Harvest UV-C Application in Regulating Grape Berry Quality Development
Authors: Zhang Kekun , Li Wanping , Ju Yanlun , Wang Xianhang , Sun Xiangyu , Fang Yulin , Chen Keqin
Published: March 2021

In this study, ultraviolet-C (UV-C) was utilized to improve the quality of post-harvest grape berries, and the transcriptomic and metabolomic basis of this improvement was elucidated. Berries of the red grape variety ‘Zicui’ and the white variety ‘Xiangfei’ were chosen to evaluate the effect of short- and long-term UV-C irradiation. Post-harvest UV-C application promoted malondialdehyde (MDA) and proline accumulation, and reduced the soluble solid content in berries. Both the variety and duration of irradiation could modulate the transcriptomic and metabolomic responses of berries to UV-C. Compared with the control, the differentially expressed genes (DEGs) identified under UV-C treatment were enriched in pathways related to metabolite accumulation, hormone biosynthesis and signal transduction, and reactive oxygen species (ROS) homeostasis. Flavonoid biosynthesis and biosynthesis of other secondary metabolites were the shared pathways enriched with differential metabolites. After long-term UV-C irradiation, cis-resveratrol accumulated in the berries of the two varieties, while the differential chalcone, dihydroflavone, flavonoid, flavanol, and tannin components primarily accumulated in ‘Xiangfei’, and some flavonols and anthocyanins primarily accumulated in ‘Zicui’. Based on an exhaustive survey, we made a summary for the effect of UV-C in regulating the quality development of post-harvest grape berries. The results of this study may help to elucidate the mechanism by which UV-C functions and support its efficient application.
Ultrasonic Freezing Reduces Protein Oxidation and Myofibrillar Gel Quality Loss of Common Carp (Cyprinus carpio) during Long-Time Frozen Storage
Authors: Sun Qinxiu , Kong Baohua , Liu Shucheng , Zheng Ouyang , Zhang Chao
Published: March 2021

Ultrasonic freezing (UF) is an effective method to increase the freezing speed and improve the quality of frozen food. The effect of UF on myofibrillar protein oxidation and gel properties of common carp (Cyprinus carpio) during frozen storage were investigated with air freezing (AF) and immersion freezing (IF) as controls. The results showed that the carbonyl and dityrosine content of UF samples were lower and the free amine content was higher than those of AF and IF samples during frozen storage indicating that UF inhibited protein oxidation caused by frozen storage. The particle size of UF myofibrillar protein was the smallest among all the groups indicating that UF inhibited the protein aggregation. The UF sample had higher G’, G” value, gel strength and gel water holding capacity than AF and IF groups showing that UF reduced the loss of protein gel properties. The gel microstructure showed that UF protein gel was characterized by smaller and finer pores than other samples, which further proves that UF inhibited loss of gel properties during frozen storage. The UF sample had shorter T2 transition time than other samples demonstrating that UF decreased the mobility of water. In general, UF is an effective method to reduce protein oxidation and gel properties loss caused by frozen storage.
Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide
Authors: Lumian Jessica E. , Jungblut Anne D. , Dillion Megan L. , Hawes Ian , Doran Peter T. , Mackey Tyler J. , Dick Gregory J. , Grettenberger Christen L. , Sumner Dawn Y.
Published: March 2021

Sulfide inhibits oxygenic photosynthesis by blocking electron transfer between H2O and the oxygen-evolving complex in the D1 protein of Photosystem II. The ability of cyanobacteria to counter this effect has implications for understanding the productivity of benthic microbial mats in sulfidic environments throughout Earth history. In Lake Fryxell, Antarctica, the benthic, filamentous cyanobacterium Phormidium pseudopriestleyi creates a 1–2 mm thick layer of 50 µmol L−1 O2 in otherwise sulfidic water, demonstrating that it sustains oxygenic photosynthesis in the presence of sulfide. A metagenome-assembled genome of P. pseudopriestleyi indicates a genetic capacity for oxygenic photosynthesis, including multiple copies of psbA (encoding the D1 protein of Photosystem II), and anoxygenic photosynthesis with a copy of sqr (encoding the sulfide quinone reductase protein that oxidizes sulfide). The genomic content of P. pseudopriestleyi is consistent with sulfide tolerance mechanisms including increasing psbA expression or directly oxidizing sulfide with sulfide quinone reductase. However, the ability of the organism to reduce Photosystem I via sulfide quinone reductase while Photosystem II is sulfide-inhibited, thereby performing anoxygenic photosynthesis in the presence of sulfide, has yet to be demonstrated.
Snaking into the Gothic: Serpentine Sensuousness in Lewis and Coleridge
Authors: Chow Jeremy
Published: March 2021

This essay charts the ways late-eighteenth-century Gothic authors repurpose natural histories of snakes to explore how reptile-human encounters are harbingers of queer formations of gender, sexuality, and empire. By looking to M.G. Lewis’s novel The Monk (1796) and his understudied short story “The Anaconda” (1808), as well as S.T. Coleridge’s Christabel (1797–1800), I centre the last five years of the eighteenth century to apprehend the interwoven nature of Gothic prose, poetry, and popular natural histories as they pertain to reptile knowledge and representations. Whereas Lewis’s short story positions the orientalised anaconda to upheave notions of empire, gender, and romance, his novel invokes the snake to signal the effusion of graphic eroticisms. Coleridge, in turn, invokes the snake-human interspecies connection to imagine female, homoerotic possibilities and foreclosures. Plaiting eighteenth-century animal studies, queer studies, and Gothic studies, this essay offers a queer eco-Gothic reading of the violating, erotic powers of snakes in their placement alongside human interlocutors. I thus recalibrate eighteenth-century animal studies to focus not on warm-blooded mammals, but on cold-blooded reptiles and the erotic effusions they afford within the Gothic imaginary that repeatedly conjures them, as I show, with queer interspecies effects.
Astilbe and Coneflower Growth as Affected by Fertilizer Rate and Substrate Volumetric Water Content
Authors: Bayer Amanda
Published: March 2021

Improved irrigation and fertilization practices, such as reduced applications, are needed to improve the sustainability of container plant production. The objective of this study was to assess growth of Visions astilbe (Astilbe chinensis ‘Visions’) and Mellow Yellow coneflower (Echinacea purpurea ‘Mellow Yellow’) grown at two controlled-release fertilizer (CRF) rates (100% or 50% of the medium bag rate) and two volumetric water contents (VWC; 40% and 18%). For coneflower, there were no significant treatment effects for height, growth index, shoot dry weight, or leaf size. There was a significant VWC effect on number of flowers with the 40% treatment having more flowers (5.6) per plant than the 18% treatment (2.7). Shoot dry weight, growth index, and leaf size of astilbe were greater for the 40% VWC treatment than the 18% VWC treatment with no fertilizer rate effect. Astilbe height and number of flowers was not significant. These results indicate that there is a species-specific effect of VWC on growth whereas reduced fertilizer applications are possible for both species without impacting growth. Although a substrate VWC of 18% is likely too low to produce salable plants, a VWC below 40% can potentially be used to support adequate growth.
What Are Kinematic and Kinetic Differences between Short and Parallel Turn in Alpine Skiing?
Authors: Bon Ivan , Očić Mateja , Cigrovski Vjekoslav , Rupčić Tomislav , Knjaz Damir
Published: March 2021

There are numerous programs worldwide adapted for alpine ski beginners and they all share the same primary goal—inclusion of skiing beginners in alpine ski schools. The final elements of ski school taught in the parallel skiing technique are parallel turn and short turn. Synchronized analysis of kinetic and kinematic parameters of the parallel turn (PT) and short turn (ST) was conducted to determine the main biomechanical differences from a standpoint of foot pressure and lower limb angles. Both elements were performed by nine male ski instructors (age 33.4 ± 8.62, height 179.52 ± 5.98 cm, weight 78.6 ± 8.88 kg). Kinetic and kinematic analysis was conducted on 180 turns, 90 for each element. Differences in kinetic and kinematic parameters between parallel and short turns were tested by a paired t-test. The main findings of our study are determined differences in the ratio of pressure distribution on the inside and the outside foot and differences in kinematic parameters of the outside leg between elements. The mentioned analysis can provide an objective insight into the complexity of each element and provide guidelines for teaching process of those elements. This study determined the reasons for higher complexity of ST compared to PT based on the objective evaluation of biomechanical factors.
Differences in Habitual Physical Activity Behavior between Students from Different Vocational Education Tracks and the Association with Cognitive Performance
Authors: Golsteijn Rianne H. J. , Gijselaers Hieronymus J. M. , Savelberg Hans H. C. M. , Singh Amika S. , de Groot Renate H. M.
Published: March 2021

Vocational education and training (VET) educates students for a broad range of occupations, which may be associated with differences in habitual physical activity behavior (PAB). Research suggests that physical activity (PA) is positively and sedentary behavior (SB) is negatively associated with cognitive performance. Therefore, we aimed to compare habitual PAB in VET students from different educational tracks and investigate its association with cognitive performance in a cross-sectional study. Students wore an ActivPAL accelerometer continuously for seven days to measure PAB. Cognitive performance was assessed with objective tests for inhibition, shifting, and updating. Hairdresser and Sports students sat significantly less than Administrative and Nursing students. Hairdresser students stood significantly more than other tracks. Admin students stood significantly less than Sports/Nursing students. Sports students moved significantly more than Hairdresser and Nursing students. Time in bed was significantly lowest in Nursing students. No significant associations between any PAB and cognitive performance were found. In general, Admin students showed the unhealthiest habitual PAB. Higher PA or lower SB neither improve nor decrease cognitive performance. Thus, future health interventions focusing on exchanging SB for PA at schools can facilitate a healthier lifestyle of VET students, especially in Admin students, without interfering with cognitive performance.
Application Intensity and Spatial Distribution of Three Major Herbicides from Agricultural and Nonagricultural Practices in the Central Plain of Thailand
Authors: Kwonpongsagoon Suphaphat , Katasila Chanokwan , Kongtip Pornpimol , Woskie Susan
Published: March 2021

The herbicides glyphosate, paraquat, and 2,4-D play a significant role in Thailand. This paper is among the first study to describe the intensity of herbicide application and illustrate how the herbicides are extensively distributed over a large area through both agricultural and nonagricultural practices. Using a quick, economical, and simplified method of Material Flow Analysis together with spatial analysis, better data for the analysis of possible environmental herbicide contamination, human exposure, and related health risks for the general public and applicators can be developed. The findings from this study showed that in the study province, about 2.2 million kg of the active ingredients from the three targeted herbicides is applied annually. Pathway flow modeling with spatial analysis identified several local hotspots of concern based on the type of herbicide and crop/activity where it was used. Cassava planting was found to have the highest herbicide application activity, whereas rice cultivation was the major contributor of total herbicide mass, due to the wide area of cultivation in the province. The herbicide most likely to be applied at rates higher than recommended was 2,4-D, particularly on cassava and sugarcane farms.
Children and Adolescent’s Perception of Media Device Use Consequences
Authors: Spina Giulia , Bozzola Elena , Ferrara Pietro , Zamperini Nicola , Marino Francesco , Caruso Cinthia , Antilici Livia , Villani Alberto
Published: March 2021

Media device (MD) use is increasing worldwide among children. Adolescents and young children spend a lot of time using MD, Internet, and social networks. The age of initial use is getting lower to 12 years old. The aim of this research is to study children’s use and perception of MD. The Italian Paediatric Society (SIP) conducted a Survey on Italian children in collaboration with Skuola.net using an online questionnaire. A total of 10,000 questionnaires were completed. Children admitted they spend more than 3 h (41%), more than 2 h (29%), more than 1 h (21%) and less than 1 h (9%) daily. Problematic MD use has been found with children using MD before sleeping (38%), during school (24%), and at wake up in the morning (21%). Addiction was documented in 14% of adolescents. Among the reported consequences, low academic outcomes, and reduced concentration (24%), neck and back pain (12%), insomnia (10%), and mood disturbances (7%) were referred. Adolescents may have a low perception of the risks related to excessive MD. The duration of time spent using media devices is a main risk factor. In this context, parents should strongly discourage excessive MD use, mainly during school, at bedtime, and wake-up. Additionally, parents should be informed and start conversations with their children on the potential negative effects of prolonged MD use.
Orthogeriatric Management: Improvements in Outcomes during Hospital Admission Due to Hip Fracture
Authors: Tarazona-Santabalbina Francisco José , Ojeda-Thies Cristina , Figueroa Rodríguez Jesús , Cassinello-Ogea Concepción , Caeiro José Ramón
Published: March 2021

Hip fractures are an important socio-economic problem in western countries. Over the past 60 years orthogeriatric care has improved the management of older patients admitted to hospital after suffering hip fractures. Quality of care in orthogeriatric co-management units has increased, reducing adverse events during acute admission, length of stay, both in-hospital and mid-term mortality, as well as healthcare and social costs. Nevertheless, a large number of areas of controversy regarding the clinical management of older adults admitted due to hip fracture remain to be clarified. This narrative review, centered in the last 5 years, combined the search terms “hip fracture”, “geriatric assessment”, “second hip fracture”, “surgery”, “perioperative management” and “orthogeriatric care”, in order to summarise the state of the art of some questions such as the optimum analgesic protocol, the best approach for treating anemia, the surgical options recommendable for each type of fracture and the efficiency of orthogeriatric co-management and functional recovery.
Examining the Associations between Indigenous Rangers, Culture and Wellbeing in Australia, 2018–2020
Authors: Wright Alyson , Yap Mandy , Jones Roxanne , Richardson Alice , Davis Vanessa , Lovett Raymond
Published: March 2021

The centrality of culture to Indigenous peoples’ health and wellbeing is becoming increasingly acknowledged in government policy. In Australia, the Indigenous Ranger program is a leading example of employment that supports increased cultural participation. In 2017, we demonstrated higher life satisfaction and family wellbeing among Indigenous Rangers compared to non-Rangers in Central Australia. Using an expanded national dataset, this present study aimed to: examine if associations between Ranger status and wellbeing continued to be observed in Central Australia; assess if these associations were observed among non-Central Australian Rangers; and, quantify the effect of mediating variables (Rangers status, cultural factors) on wellbeing outcomes. We analyzed Mayi Kuwayu baseline data (n = 9691 Aboriginal and Torres Strait Islander people) and compared participants who identified as past or currently employed Rangers compared to non-Rangers across two geographic locations (Central Australia, non-Central Australia). Ranger participation was significantly associated with very high life satisfaction and family wellbeing in Central Australia (high life satisfaction PR 1.31, 95% CI 1.09–1.57, and family wellbeing (PR 1.17, 95% CI 1.01–1.36) and non-Central Australia (high life satisfaction PR 1.29, 95% CI 1.06–1.57), family wellbeing (PR 1.37, 95% CI 1.14–1.65). These findings concord with those observed in the 2017 proof-of-concept study. Additionally, we found that Ranger status partially mediated the relationships between existing cultural practices (first language as your Indigenous language and living on your country) and the two wellbeing outcomes. Current cultural practices, spending time on country and speaking your Aboriginal language, also partially mediated the associations between Ranger status and high life satisfaction, and between Ranger status and high family wellbeing. This analysis supports evidence that both Ranger employment and cultural participation are contributors to wellbeing. Ranger work is not only good for land, but it is good for people. As such, determining policies that mutually acknowledge and enhance culture, health and wellbeing will likely have additional benefits for the broader Aboriginal and Torres Strait Islander population.
Role of Hybrid Deep Neural Networks (HDNNs), Computed Tomography, and Chest X-rays for the Detection of COVID-19
Authors: Irfan Muhammad , Iftikhar Muhammad Aksam , Yasin Sana , Draz Umar , Ali Tariq , Hussain Shafiq , Bukhari Sarah , Alwadie Abdullah Saeed , Rahman Saifur , Glowacz Adam , Althobiani Faisal
Published: March 2021

COVID-19 syndrome has extensively escalated worldwide with the induction of the year 2020 and has resulted in the illness of millions of people. COVID-19 patients bear an elevated risk once the symptoms deteriorate. Hence, early recognition of diseased patients can facilitate early intervention and avoid disease succession. This article intends to develop a hybrid deep neural networks (HDNNs), using computed tomography (CT) and X-ray imaging, to predict the risk of the onset of disease in patients suffering from COVID-19. To be precise, the subjects were classified into 3 categories namely normal, Pneumonia, and COVID-19. Initially, the CT and chest X-ray images, denoted as ‘hybrid images’ (with resolution 1080 × 1080) were collected from different sources, including GitHub, COVID-19 radiography database, Kaggle, COVID-19 image data collection, and Actual Med COVID-19 Chest X-ray Dataset, which are open source and publicly available data repositories. The 80% hybrid images were used to train the hybrid deep neural network model and the remaining 20% were used for the testing purpose. The capability and prediction accuracy of the HDNNs were calculated using the confusion matrix. The hybrid deep neural network showed a 99% classification accuracy on the test set data.
Microbiological Surveillance of Endoscopes in a Southern Italian Transplantation Hospital: A Retrospective Study from 2016 to 2019
Authors: Marchese Valentina , Di Carlo Daniele , Fazio Gaetano , Gioè Santi Mauro , Luca Angelo , Alduino Rossella , Rizzo Monica , Tuzzolino Fabio , Monaco Francesco , Conaldi Pier Giulio , Douradinha Bruno , Di Martino Giuseppina
Published: March 2021

Endoscopes are medical instruments that are used routinely in health structures. Due to their invasive nature and contact with many patients, they may cause hospital-acquired infections if not disinfected correctly. To ensure a high-level disinfection procedure or reprocessing, since the methods currently adopted in our institute are adequate, we evaluated retrospectively the presence of microorganisms in our endoscopes after reprocessing. Microbiological surveillance was performed from January 2016 to December 2019 in the instruments in use in our endoscopic room after reprocessing. In total, 35 endoscopes (3 duodenoscopes, 3 echoendoscopes, 12 bronchoscopes, 5 colonoscopes, and 12 gastroscopes) were evaluated for the presence of microorganisms, including multidrug-resistant pathogens and indicator microorganisms (IMOs). Our procedures were in agreement with an internal protocol based on Italian, international, and the Center for Disease Control and Prevention (CDC) recommendations. Of a total of 811 samples, 799 (98.5%) complied with the regulatory guidelines, while 9 (1.1%) were positive for IMOs, and 3 (0.4%) displayed more than 10 colony-forming units (CFU) of environmental and commensal pathogens. Our results show that the internal reprocessing protocol is very efficient, leading to a very low number of observed contaminations, and it could be easily implemented by other health facilities that face a huge number of hospital-acquired infections due to incorrectly disinfected endoscopes.
Facing COVID-19 Challenges: 1st-Year Students’ Experience with the Romanian Hybrid Higher Educational System
Authors: Potra Sabina , Pugna Adrian , Pop Mădălin-Dorin , Negrea Romeo , Dungan Luisa
Published: March 2021

First-year students undergo several transformations like the transition from high school to university schedules, teaching methods, and discipline specificities to social changes that come with meeting new colleagues or moving to other locations far from family and friends. The COVID-19 outbreak brought additional concerns and uncertainties once educational systems implemented distance learning schemes for public health management. Nevertheless, higher educational organizations preferred to implement hybrid approaches for student engagement motivation and high dropout rate avoidance. In Romania, such an educational system has been applied with caution. Since the pandemic needs to be understood as an opportunity for adaptation and education improvements, the purpose of the present paper is to uncover lessons learned and to develop a systematized model based on students’ perception regarding face-to-face, online and hybrid systems. For this reason, a grounded theory approach has been preferred. Thus, 149 Romanian students enrolled in the first year in engineering specialities with ages between 18–26 years both male (50.3%) and female (49.7%) have answered the research questions in an online environment due to pandemic restrictions. Based on the online asynchronous student interviews, 220 codes and a further 13 categories have been developed. After a pertinent analysis of the relationships between categories and relevant literature sorting, a theoretical model for the Romanian higher educational current pandemic situation has been proposed. The main face-to-face and online education characteristics are outlined, the hybrid approach becoming a bridge between the two systems. Management implications are outlined together with further research directions.
Effects of an Extracurricular Anger Self-Control Program for Nursing Students
Authors: Jun Won Hee , Choi Eun Joung , Cho Hyun-Mee
Published: March 2021

Nursing students often experience anger in response to stress and suppress their anger instead of actively controlling it. Therefore, the anger self-control programs that can manage nursing students’ anger level and dysfunctional anger expression are needed. The aim of this study is to investigate the effects of an anger self-control program on trait anger, anger expression style, grateful disposition, and depression among nursing students. The study used a quasi-experimental study with a nonequivalent control group and a non-synchronized design. Participants were 29 nursing students who were assigned to intervention and control groups. Compared to the control group, the intervention group showed significantly decreased mean scores for the trait anger, anger-in, and anger-out anger expression styles, and increased mean scores for the anger-control anger expression style and grateful disposition. Anger self-control programs might be usefully applied as extracurricular anger-management programs for nursing students.
Characteristics of the Electrophysiological Properties of Neuromuscular Motor Units and Its Adaptive Strategy Response in Lower Extremity Muscles for Seniors with Pre-Sarcopenia: A Preliminary Study
Authors: Hu Chia-Han , Yang Chia-Chi , Tu Shihfan Jack , Huang Ing-Jer , Ganbat Danaa , Guo Lan-Yuen
Published: March 2021

Older adults with sarcopenia, which is an aging-related phenomenon of muscle mass loss, usually suffer from decreases in both strength and functional performance. However, the causality between function loss and physiological changes is unclear. This study aimed to explore the motor unit characteristics of the neurological factors between normal subjects and those with sarcopenia. Five risk-sarcopenia (age: 66.20 ± 4.44), five healthy (age: 69.00 ± 2.35), and twelve young (age: 21.33 ± 1.15) participants were selected. Each participant performed knee extension exercises at a 50% level of maximal voluntary isometric contraction. Next, electromyogram (EMG) signals were collected, and information on each parameter—e.g., motor unit number, recruitment threshold, the slope of the mean firing rate to recruitment threshold, y-intercept, firing rate per unit force, and mean motor unit firing rate (MFR)—was extracted to analyze muscle fiber discrimination (MFD). Meanwhile, force variance was used to observe the stability between two muscle groups. The results suggested that there was no difference between the three groups for motor unit number, recruitment threshold, y-intercept, mean firing rate, and motor unit discrimination (p > 0.05). However, the slope of MFR and firing rate per unit force in the risk-sarcopenia group were significantly higher than in the young group (p < 0.05). Regarding muscle performance, the force variance in the non-sarcopenia group was significantly higher than the young group (p < 0.05), while the risk-sarcopenia group showed a higher trend than the young group. This study demonstrated some neuromuscular characters between sarcopenia and healthy elderly and young people when performing the same level of leg exercise tasks. This difference may provide some hints for discovering aging-related strength and function loss. Future studies should consider combining the in vivo measurement of muscle fiber type to clarify whether this EMG difference is related to the loss of muscle strength or mass before recruiting symptomatic elderly participants for further investigation.
Nasal Morphology and Its Correlation to Craniofacial Morphology in Lateral Cephalometric Analysis
Authors: Jankowska Agnieszka , Janiszewska-Olszowska Joanna , Grocholewicz Katarzyna
Published: March 2021

Nose shape, size, and inclination influence facial appearance, but few studies concern the relationship between the nasal profile and craniofacial structures. The objective of this study was to analyze association of nasal cephalometric variables with skeletal structures, age, and sex. Cephalometric and nasal analysis was performed in 386 Polish orthodontic patients (aged 9–25 years). Student t-test and Mann–Whitney test were used to compare quantitative variables and Pearson’s or Spearman’s correlation coefficients—to find correlations. Soft tissue facial convexity angle correlates to Holdaway ratio, ANB (A-Nasion-B), and Wits appraisal. Nasal dorsum axis, nose length, nose depth (1) and nose depth (2), nose hump, lower dorsum convexity, and columella convexity increase with age. Nasal base angle, nasolabial angle, nasomental angle, soft tissue facial convexity and nasal bone angle decrease with age. Nasal base angle and nasomental angle are smaller in females. Thus, a relationship exists between nasal morphology and sagittal jaw configuration. Nasal parameters significantly change with age. Sexual dimorphism characterizes nasal bone angle and nasomental angle.
A ‘Dilute and Shoot’ Liquid Chromatography-Mass Spectrometry Method for Multiclass Drug Analysis in Pre-Cut Dried Blood Spots
Authors: Mainero Rocca Lucia , L’Episcopo Nunziata , Gordiani Andrea , Vitali Matteo , Staderini Alessandro
Published: March 2021

Drugs able to affect the auditory and nervous systems and consumed by workers to treatdifferent pathologies can represent a possible source of risk in the work environment. All the target compounds involved in the presented project show ototoxic and/or narcoleptic side effects and, for these reasons, occupational safety organizations have recognized them as potential causes of work injuries. A multiclass method for the analysis of 15 drugs among the most widespread worldwide (belonging to nine different classes including antihistamines, beta-blockers, antidepressants, Z-drugs and opioids), was developed and validated. This study describes a rapid, sensitive and effective method to analyse these substances in whole blood using tailored pre-cut dried blood spots. Detection was achieved with a triple quadrupole mass spectrometer after an easy and simple ‘dilute and shoot’ solubilisation followed by an UPLC separation. All the issues linked to the use of the dried blood spots and whole blood, such as haematocrit variability, volumetric evaluation and sample carrier choice were carefully studied and managed during method development. From the validation study results it emerged that this approach can be deemed successful thanks to its few pg µL−1 LOQs, good linear intervals, absolute recoveries of no less than 75%, an almost negligible matrix effect and accuracy and precision in line with the European and American guidelines for validation. All the obtained goals have been specifically pursued in order to encourage method diffusion as a primary prevention intervention, even in small private workplaces.
Combined Genome, Transcriptome and Metabolome Analysis in the Diagnosis of Childhood Cerebellar Ataxia
Authors: Ching-López Ana , Martinez-Gonzalez Luis Javier , Arrabal Luisa , Sáiz Jorge , Gavilán Ángela , Barbas Coral , Lorente Jose Antonio , Roldán Susana , Sánchez Maria José , Gutierrez-Ríos Purificacion
Published: March 2021

Ataxia in children is a common clinical sign of numerous neurological disorders consisting of impaired coordination of voluntary muscle movement. Its most common form, cerebellar ataxia, describes a heterogeneous array of neurologic conditions with uncountable causes broadly divided as acquired or genetic. Numerous genetic disorders are associated with chronic progressive ataxia, which complicates clinical management, particularly on the diagnostic stage. Advances in omics technologies enable improvements in clinical practice and research, so we proposed a multi-omics approach to aid in the genetic diagnosis and molecular elucidation of an undiagnosed infantile condition of chronic progressive cerebellar ataxia. Using whole-exome sequencing, RNA-seq, and untargeted metabolomics, we identified three clinically relevant mutations (rs141471029, rs191582628 and rs398124292) and an altered metabolic profile in our patient. Two POLR1C diagnostic variants already classified as pathogenic were found, and a diagnosis of hypomyelinating leukodystrophy was achieved. A mutation on the MMACHC gene, known to be associated with methylmalonic aciduria and homocystinuria cblC type, was also found. Additionally, preliminary metabolome analysis revealed alterations in our patient’s amino acid, fatty acid and carbohydrate metabolism. Our findings provided a definitive genetic diagnosis reinforcing the association between POLR1C mutations and hypomyelinating leukodystrophy and highlighted the relevance of multi-omics approaches to the disease.
Cannabidiol and Other Cannabinoids in Demyelinating Diseases
Authors: Navarrete Carmen , García-Martín Adela , Rolland Alain , DeMesa Jim , Muñoz Eduardo
Published: March 2021

A growing body of preclinical evidence indicates that certain cannabinoids, including cannabidiol (CBD) and synthetic derivatives, may play a role in the myelinating processes and are promising small molecules to be developed as drug candidates for management of demyelinating diseases such as multiple sclerosis (MS), stroke and traumatic brain injury (TBI), which are three of the most prevalent demyelinating disorders. Thanks to the properties described for CBD and its interesting profile in humans, both the phytocannabinoid and derivatives could be considered as potential candidates for clinical use. In this review we will summarize current advances in the use of CBD and other cannabinoids as future potential treatments. While new research is accelerating the process for the generation of novel drug candidates and identification of druggable targets, the collaboration of key players such as basic researchers, clinicians and pharmaceutical companies is required to bring novel therapies to the patients.
The Role of the Gut Microbiota in the Gut–Brain Axis in Obesity: Mechanisms and Future Implications
Authors: van Son Jamie , Koekkoek Laura L. , La Fleur Susanne E. , Serlie Mireille J. , Nieuwdorp Max
Published: March 2021

Interaction between the gut and the brain is essential for energy homeostasis. In obesity, this homeostasis is disrupted, leading to a positive energy balance and weight gain. Obesity is a global epidemic that affects individual health and strains the socioeconomic system. Microbial dysbiosis has long been reported in obesity and obesity-related disorders. More recent literature has focused on the interaction of the gut microbiota and its metabolites on human brain and behavior. Developing strategies that target the gut microbiota could be a future approach for the treatment of obesity. Here, we review the microbiota–gut–brain axis and possible therapeutic options.
EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers
Authors: Wilmowicz Emilia , Kućko Agata , Pokora Wojciech , Kapusta Małgorzata , Jasieniecka-Gazarkiewicz Katarzyna , Tranbarger Timothy John , Wolska Magdalena , Panek Katarzyna
Published: March 2021

Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield. It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells? Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ. EPIP stimulates the downstream elements of the pathway—HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation. The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase. A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism. Notably, the formation of a signaling molecule—phosphatidic acid is induced locally in EPIP-treated AZ. Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.
Molecular Alterations in Sporadic and SOD1-ALS Immortalized Lymphocytes: Towards a Personalized Therapy
Authors: Lastres-Becker Isabel , Porras Gracia , Arribas-Blázquez Marina , Maestro Inés , Borrego-Hernández Daniel , Boya Patricia , Cerdán Sebastián , García-Redondo Alberto , Martínez Ana , Martin-Requero Ángeles
Published: March 2021

Amyotrophic lateral sclerosis (ALS) is a fatal neurological condition where motor neurons (MNs) degenerate. Most of the ALS cases are sporadic (sALS), whereas 10% are hereditarily transmitted (fALS), among which mutations are found in the gene that codes for the enzyme superoxide dismutase 1 (SOD1). A central question in ALS field is whether causative mutations display selective alterations not found in sALS patients, or they converge on shared molecular pathways. To identify specific and common mechanisms for designing appropriate therapeutic interventions, we focused on the SOD1-mutated (SOD1-ALS) versus sALS patients. Since ALS pathology involves different cell types other than MNs, we generated lymphoblastoid cell lines (LCLs) from sALS and SOD1-ALS patients and healthy donors and investigated whether they show changes in oxidative stress, mitochondrial dysfunction, metabolic disturbances, the antioxidant NRF2 pathway, inflammatory profile, and autophagic flux. Both oxidative phosphorylation and glycolysis appear to be upregulated in lymphoblasts from sALS and SOD1-ALS. Our results indicate significant differences in NRF2/ARE pathway between sALS and SOD1-ALS lymphoblasts. Furthermore, levels of inflammatory cytokines and autophagic flux discriminate between sALS and SOD1-ALS lymphoblasts. Overall, different molecular mechanisms are involved in sALS and SOD1-ALS patients and thus, personalized medicine should be developed for each case.
The Plants of the Asteraceae Family as Agents in the Protection of Human Health
Authors: Rolnik Agata , Olas Beata
Published: March 2021

The Asteraceae family is one of the largest flowering plant families, with over 1600 genera and 25,000 species worldwide. Some of its most well-known taxa are lettuce, chicory, artichoke, daisy and dandelion. The members of the Asteraceae have been used in the diet and for medicine for centuries. Despite their wide diversity, most family members share a similar chemical composition: for example, all species are good sources of inulin, a natural polysaccharide with strong prebiotic properties. They also demonstrate strong antioxidant, anti-inflammatory and antimicrobial activity, as well as diuretic and wound healing properties. Their pharmacological effects can be attributed to their range of phytochemical compounds, including polyphenols, phenolic acids, flavonoids, acetylenes and triterpenes. One such example is arctiin: a ligand with numerous antioxidant, antiproliferative and desmutagenic activities. The family is also a source of sesquiterpene lactones: the secondary metabolites responsible for the bitter taste of many plants. This mini review examines the current state of literature regarding the positive effect of the Asteraceae family on human health.
Genetic Involvement of Mycobacterium avium Complex in the Regulation and Manipulation of Innate Immune Functions of Host Cells
Authors: Shin Min-Kyoung , Shin Sung Jae
Published: March 2021

Mycobacterium avium complex (MAC), a collection of mycobacterial species representing nontuberculous mycobacteria, are characterized as ubiquitous and opportunistic pathogens. The incidence and prevalence of infectious diseases caused by MAC have been emerging globally due to complications in the treatment of MAC-pulmonary disease (PD) in humans and the lack of understating individual differences in genetic traits and pathogenesis of MAC species or subspecies. Despite genetically close one to another, mycobacteria species belonging to the MAC cause diseases to different host range along with a distinct spectrum of disease. In addition, unlike Mycobacterium tuberculosis, the underlying mechanisms for the pathogenesis of MAC infection from environmental sources of infection to their survival strategies within host cells have not been fully elucidated. In this review, we highlight unique genetic and genotypic differences in MAC species and the virulence factors conferring the ability to MAC for the tactics evading innate immune attacks of host cells based on the recent advances in genetic analysis by exemplifying M. avium subsp. hominissuis, a major representative pathogen causing MAC-PD in humans. Further understanding of the genetic link between host and MAC may contribute to enhance host anti-MAC immunity, but also provide novel therapeutic approaches targeting the pangenesis-associated genes of MAC.
Central Apparatus, the Molecular Kickstarter of Ciliary and Flagellar Nanomachines
Authors: Samsel Zuzanna , Sekretarska Justyna , Osinka Anna , Wloga Dorota , Joachimiak Ewa
Published: March 2021

Motile cilia and homologous organelles, the flagella, are an early evolutionarily invention, enabling primitive eukaryotic cells to survive and reproduce. In animals, cilia have undergone functional and structural speciation giving raise to typical motile cilia, motile nodal cilia, and sensory immotile cilia. In contrast to other cilia types, typical motile cilia are able to beat in complex, two-phase movements. Moreover, they contain many additional structures, including central apparatus, composed of two single microtubules connected by a bridge-like structure and assembling numerous complexes called projections. A growing body of evidence supports the important role of the central apparatus in the generation and regulation of the motile cilia movement. Here we review data concerning the central apparatus structure, protein composition, and the significance of its components in ciliary beating regulation.