bluuebunny/crossref_metadata_2025 huggingface dataset:
Hsa_circ_0046523 Mediates an Immunosuppressive Tumor Microenvironment by Regulating MiR-148a-3p/PD-L1 Axis in Pancreatic Cancer
Authors: Fu Xiaowei , Sun Gen , Tu Shuju , Fang Kang , Xiong Yuanpeng , Tu Yi , Zha Ming , Xiao Tao , Xiao Weidong
Published: May 2022

Background

Circular RNAs (circRNAs) are a novel type of non-coding RNA, play an important role in the progression of tumors. However, the function and mechanism of circRNAs in regulating immune microenvironment of pancreatic cancer (PC) remain largely unclear.

Methods

The effects of hsa_circ_0046523 expression on proliferation, migration and invasion of PC cells were analyzed by CCK8 and Transwell assays. Flow cytometry was used to detect the proportion of CD4+ T cells, CD8+ T cells and Tregs in peripheral blood mononuclear cells (PBMCs) after co-culture, and the apoptosis, depletion and function of CD8+ T cells. The expression levels of immunoregulatory cytokines were detected by enzyme linked immunosorbent assay (ELISA). The dual-luciferase reporter was performed to determine the interaction between hsa_circ_0046523, miR-148a-3p, and PD-L1. Rescue experiments and PD-L1 blocking experiments were employed to investigate whether hsa_circ_0046523 exerts its biological function by miR-148a-3p/PD-L1 in PC. Furthermore, an immunocompetent murine PC model was established to confirm these findings.

Results

Hsa_circ_0046523 expression was remarkably upregulated in PC tissues and cell lines. Moreover, high expression of hsa_circ_0046523 was correlated with advanced pathological stage and poorer prognosis. Hsa_circ_0046523 overexpression promoted the proliferation, migration and invasion of PC cells in vitro. Co-culture experiments confirmed that forced expression of hsa_circ_0046523 could decrease the proportion of CD4+ and CD8+ T cells, as well as increase the proportion of Tregs among peripheral blood mononuclear cells (PBMCs). Meanwhile, hsa_circ_0046523 overexpression promoted the apoptosis and exhaustion of CD8+ T cells, inhibited CD8+ T cell function, increased the secretion of immunosuppressive cytokines IL-10 and TGF-β, and decreased the secretion of immune effector cytokines IFN-γ and IL-2 among PBMCs. Mechanistically, hsa_circ_0046523 exerted its biological function by binding to miR-148a-3p to upregulate PD-L1 expression in PC. Moreover, these immune modulating functions of miR-148a-3p/PD-L1 axis were also confirmed in an immunocompetent murine PC model.

Conclusions

Our study suggests that hsa_circ_0046523/miR-148a-3p/PD-L1 regulatory axis mediates PC immunosuppressive microenvironment and these molecules are expected to be new targets for remodeling tumor immune microenvironment of PC.
Research Progress on RET Fusion in Non-Small-Cell Lung Cancer
Authors: Zhao Lu , Mei Qingyun , Yu Yongchao , Wang Na , Zhang Dou , Liao Dongying , Zuo Jinhui , Xie Hongxia , Jia Yingjie , Kong Fanming
Published: May 2022

Great progress has been made in the treatment of driver gene-positive Non- Small Cell Lung Cancer (NSCLC) in recent years. RET fusion was seen in 0.7% to 2% of NSCLC and was associated with younger age and never-smoker status. The pralsetinib and selpercatinib for RET fusion NSCLC was recommended by the 2021 NSCLC treatment guidelines. This review outlines the research progress in the treatment of RET fusion NSCLC, identifies current challenges and describes proposals for improving the outlook for these patients.
The Lived Experience of Resilience in Parents of Children With Cancer: A Phenomenological Study
Authors: Luo Yuanhui , Li Ho Cheung William , Xia Wei , Cheung Ankie Tan , Ho Laurie Long Kwan , Chung Joyce Oi Kwan
Published: May 2022

Background

Resilience is vital in parents of children with cancer as it can promote parental well-being and minimize maladaptation in the face of the children's cancer. Although existing quantitative studies investigated the influence factors of resilience in the parents, it has not been fully explored about the factors contributing to the resilience of parents and how they respond to and cope with their children's cancer.

Objective

To investigate the lived experience of resilience in the parents of children with cancer from a qualitative perspective to complement existing findings in quantitative studies.

Methods

A phenomenological approach was used. Purposive sampling was performed to recruit parents of children with cancer from two tertiary hospitals in mainland China, followed by one-to-one semi-structured interviews. All of the interviews were audio-recorded and data were analyzed using thematic analysis.

Results

Twenty-three parents, comprising 15 mothers and eight fathers, of children with cancer participated in the interview. Four themes were identified: positive and negative experiences of their children's disease, going through hardships, perceived competence and perceived social support. The most prominent facilitating factor of resilience was the presence of positive attitudes toward the children's cancer, while low level of confidence was the main obstacle.

Conclusion

This study identified certain factors that affect resilience in parents of children with cancer. The findings of this study provide important implications for the development of targeted resilience training programs to enhance resilience in parents of children with cancer. It is crucial for future interventions to focus on cultivating parental resilience to promote parents' mental well-being and improve their quality of life.

ClinicalTrials.gov ID

NCT03631485; URL: https://clinicaltrials.gov/ct2/show/NCT03631485.
Time to Treatment Intensification in Patients Receiving DPP4 Inhibitors Versus Sulfonylureas as the First Add-On to Metformin Monotherapy: A Retrospective Cohort Study
Authors: Roberto Giuseppe , Girardi Anna , Barone-Adesi Francesco , Pecere Alessandro , Ientile Valentina , Bartolini Claudia , Da Cas Roberto , Spila-Alegiani Stefania , Ferrajolo Carmen , Francesconi Paolo , Trifirò Gianluca , Poluzzi Elisabetta , Baccetti Fabio , Gini Rosa
Published: May 2022

Background: To verify whether, in patients on metformin (MET) monotherapy for type 2 diabetes (T2D), the add-on of a dipeptidyl peptidase inhibitor (DPP4i) compared to a sulfonylurea (SU) can delay the time to the subsequent treatment intensification (TI).

Methods: Population-based administrative data banks from four Italian geographic areas were used. Patients aged ≥18 years on MET monotherapy receiving first DPP4i or SU dispensing between 2008 and 2015 (cohort entry) were followed up to the occurrence of TI (insulin dispensing or add-on of a third non-insulin hypoglicemic >180 days after cohort entry), treatment discontinuation, switch, cancer, death, TI occurrence within, end of data availability, end of study period (31 December 2016), whichever came first. Patients on MET + DPP4i were matched 1:1 with those on MET + SU by sex, age, year of cohort entry, and data bank. Hazard Ratio (HR) and 95% confidence intervals (95%CI) were estimated using multivariable Cox regression model including matching variables and potential confounders measured at baseline. Different sensitivity analyses were performed: i) matching at 180 days after cohort entry, ii) intent to treat (ITT) analysis, iii) matching by duration of MET monotherapy, iv) matching by propensity score.

Results: The matched study cohort included 10,600 patients. Overall, 763 TI were observed (4.5/100 person-years; mean follow-up = 1.6 years). The primary analysis showed no difference in time to TI between the two groups (HR = 1.02; 95% CI = 0.88–1.19). Sensitivity analyses confirmed this result, except from the ITT analysis (HR = 1.27; 1.13–1.43).

Conclusion: The use of a DPP4i rather than a SU as add-on to MET monotherapy was not associated with a delay in treatment intensification.
Synthesis, Screening and Characterization of Novel Potent Arp2/3 Inhibitory Compounds Analogous to CK-666
Authors: Fokin Artem I. , Chuprov-Netochin Roman N. , Malyshev Alexander S. , Romero Stéphane , Semenova Marina N. , Konyushkin Leonid D. , Leonov Sergey V. , Semenov Victor V. , Gautreau Alexis M.
Published: May 2022

Branched actin networks polymerized by the Actin-related protein 2 and 3 (Arp2/3) complex play key roles in force generation and membrane remodeling. These networks are particularly important for cell migration, where they drive membrane protrusions of lamellipodia. Several Arp2/3 inhibitory compounds have been identified. Among them, the most widely used is CK-666 (2-Fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]-benzamide), whose mode of action is to prevent Arp2/3 from reaching its active conformation. Here 74 compounds structurally related to CK-666 were screened using a variety of assays. The primary screen involved EdU (5-ethynyl-2′-deoxyuridine) incorporation in untransformed MCF10A cells. The resulting nine positive hits were all blocking lamellipodial protrusions and cell migration in B16-F1 melanoma cells in secondary screens, showing that cell cycle progression can be a useful read-out of Arp2/3 activity. Selected compounds were also characterized on sea urchin embryos, where Arp2/3 inhibition yields specific phenotypes such as the lack of triradiate spicules and inhibition of archenteron elongation. Several compounds were filtered out due to their toxicity in cell cultures or on sea urchin development. Two CK-666 analogs, 59 (N-{2-[5-(Benzyloxy)-2-methyl-1H-indol-3-yl] ethyl}-3-bromobenzamide) and 69 (2,4-Dichloro-N-[2-(7-chloro-2-methyl-1H-indol-3-yl) ethyl]-5-[(dimethylamino) sulfonyl] benzamide), were active in all assays and significantly more efficient in vivo than CK-666. These best hits with increased in vivo potency were, however, slightly less efficient in vitro than CK-666 in the classical pyrene-actin assay. Induced-fit docking of selected compounds and their possible metabolites revealed interaction with Arp2/3 that suppresses Arp2/3 activation. The data obtained in our screening validated the applicability of original assays for Arp2/3 activity. Several previously unexplored CK-666 structural analogs were found to suppress Arp2/3 activation, and two of them were identified as Arp2/3 inhibitors with improved in vivo efficiency.
Persistent Activation of Autophagy After Cisplatin Nephrotoxicity Promotes Renal Fibrosis and Chronic Kidney Disease
Authors: Fu Ying , Xiang Yu , Wu Wenwen , Cai Juan , Tang Chengyuan , Dong Zheng
Published: May 2022

Autophagy, a highly conserved catabolic pathway in eukaryotic cells, contributes to the maintenance of the homeostasis and function of the kidney. Upon acute kidney injury (AKI), autophagy is activated in renal tubular cells to act as an intrinsic protective mechanism. However, the role of autophagy in the development of chronic kidney pathologies including renal fibrosis after AKI remains unclear. In this study, we detected a persistent autophagy activation in mouse kidneys after nephrotoxicity of repeated low dose cisplatin (RLDC) treatment. 3-methyladenine (3-MA) and chloroquine (CQ), respective inhibitors of autophagy at the initiation and degradation stages, blocked autophagic flux and improved kidney repair in post-RLDC mice, as indicated by kidney weight, renal function, and less interstitial fibrosis. In vitro, RLDC induced a pro-fibrotic phenotype in renal tubular cells, including the production and secretion of pro-fibrotic cytokines. Notably, autophagy inhibitors blocked RLDC-induced secretion of pro-fibrotic cytokines in these cells. Together, the results indicate that persistent autophagy after AKI induces pro-fibrotic cytokines in renal tubular cells, promoting renal fibrosis and chronic kidney disease.
Synergistic and Additive Interactions of Zhongshengmycin to the Chemical Insecticide Pymetrozine for Controlling Nilaparvata lugens (Hemiptera: Delphacidae)
Authors: Zhao Ruoying , Li Danting , Wang Xinlong , Li Zhong , Yu Xiaoping , Shentu Xuping
Published: May 2022

Management of the rice brown planthopper Nilaparvata lugens Stål is challenging because it can rapidly adapt to new pesticides within several generations. Combined use of chemical insecticides and antimicrobials was proposed as an alternative strategy to control N. lugens. Our previous experiments identified two effective agents (chemical insecticide: pymetrozine and antimicrobial: zhongshengmycin) that act on different targets in N. lugens. However, conditions and effectiveness of combinations of antimicrobials and insecticides against N. lugens are still unknown. Here, we evaluated separate and combined effects of pymetrozine and zhongshengmycin on third instar nymphs of N. lugens under laboratory and greenhouse conditions. Results showed that zhongshengmycin exerts significant inhibitory effects on the three endosymbionts Pichia guilliermondii, Cryptococcus peneaus, and Pichia anomala cultured in vitro of N. lugens. Combinations of pymetrozine and zhongshengmycin under laboratory conditions produced additive or synergistic effects on N. lugens and caused higher mortality in third instar nymphs than either of them used alone. Experiments under greenhouse conditions further demonstrated that effective component quality ratio of pymetrozine to zhongshengmycin of 1:10 and 1:40 with co-toxicity coefficients of 221.63 and 672.87, respectively, also produced significant synergistic effects against N. lugens. Our results indicated that chemical insecticides combined with antimicrobials may provide a potential novel strategy for controlling N. lugens by inhibiting its endosymbionts.
Unravelling Trait–Environment Relationships at Local and Regional Scales in Temperate Forests
Authors: Da Rihan , Hao Minhui , Qiao Xuetao , Zhang Chunyu , Zhao Xiuhai
Published: May 2022

Understanding the trait–environment relationships has been a core ecological research topic in the face of global climate change. However, the strength of trait–environment relationships at the local and regional scales in temperate forests remains poorly known. In this study, we investigated the local and regional scale forest plots of the natural broad-leaved temperate forest in northeastern China, to assess what extent community-level trait composition depends on environmental drivers across spatial scales. We measured five key functional traits (leaf area, specific leaf area, leaf carbon content, leaf nitrogen content, and wood density) of woody plant, and quantified functional compositions of communities by calculating the “specific” community-weighted mean (CWM) traits. The sum of squares decomposition method was used to quantify the relative contribution of intraspecific trait variation to total trait variation among communities. Multiple linear regression model was then used to explore the community-level trait–environment relationships. We found that (i) intraspecific trait variation contributed considerably to total trait variation and decreased with the spatial scale from local to regional; (ii) functional composition was mainly affected by soil and topography factors at the local scale and climate factor at the regional scale, while explaining that variance of environment factors were decreased with increasing spatial scale; and (iii) the main environment driver of functional composition was varied depending on the traits and spatial scale. This work is one of the few multi-scale analyses to investigate the environmental drivers of community functional compositions. The extent of intraspecific trait variation and the strength of trait–environment relationship showed consistent trends with increasing spatial scale. Our findings demonstrate the influence of environmental filtering on both local- and regional-scale temperate forest communities, and contribute to a comprehensive understanding of trait–environment relationships across spatial scales.
Citrus Irrigation With Desalinated Seawater Under a Climate Change Scenario
Authors: Navarro Josefa María , Antolinos Vera , Robles Juan Miguel , Botía Pablo
Published: May 2022

In arid and semiarid regions, the current lack of natural water resources is driving the use of alternative sources for crop irrigation, such as desalinated seawater (DSW). However, the use of DSW could affect the crop productivity due to its chemical composition (predominance of phytotoxic ions: Na+, Cl−, and B). Citrus species are classified as salt and boron-sensitive; however, the rootstock plays a fundamental role in the tree’s tolerance of abiotic stresses. One-year-old ‘Verna’ lemon trees grafted on two rootstocks (CM, Citrus macrophylla, and SO, sour orange) were used. These rootstocks differ in their salinity and boron tolerance, SO being more tolerant than CM. The experiment was carried out at high temperature (35/27°C), and the plants were irrigated with three types of water supplemented with Hoagland nutrients: DSW, DLB (DSW with low boron), and Control (distilled water). The plants were irrigated three times per week and harvested 7 months after the treatments started. The response to high levels of Cl−, Na+, and B was rootstock-dependent. Under the high temperature conditions, the growth of plants grafted on SO was not affected by DSW, and these plants did not reach the Cl− threshold of phytotoxicity, so the decrease in the shoot growth of plants grafted on CM due to DSW irrigation was related more to Cl− rather than the foliar Na+ accumulation. Plants grafted on SO and irrigated with DSW accumulated more B than those grafted on CM, surpassing the threshold of phytotoxicity and producing greater oxidative stress. As the growth of these plants was not reduced, the effects of DSW on plant growth were not directly related to the concentration of B and there must be some mechanisms that allow these plants to withstand the negative effects of high foliar B, such as the increased levels of quaternary ammonium compounds. Since the response of citrus plants to DSW depended on the rootstock, the results obtained in this experiment, using DSW at high temperature, could be useful for the future management of citrus crops, because climate change will increase temperatures and exacerbate the scarcity of water resources in citrus-growing areas.
Exploring the Role of English as a Foreign Language Receptive Skills and Learning Strategy Usage in the Ability to Acquire and Apply Knowledge at the Beginning of Higher Education
Authors: Magyar Andrea , Habók Anita , Molnár Gyöngyvér
Published: May 2022

Foreign language learning plays a prominent role in the world today not only for communication across borders, but also for the potential benefits of other learning skills. The main objective of this research is to examine and explore the relationship between first-year full-time undergraduate students’ (N = 1,257) English as a foreign language (EFL) reading and listening achievement and learning strategy preferences in relation to knowledge acquisition and knowledge application. Our results show that students achieved significantly better on listening tasks than on reading tasks and that their knowledge acquisition performance was higher than their knowledge application achievement. The majority of the participants reported that they usually or always employ learning strategies, with the most preferred strategy type being the control strategy. The structural model shows that language learning, and knowledge acquisition and application are strongly interrelated; moreover, the level of use of memorization and elaboration strategies directly affects both knowledge acquisition and application skills. This suggests that EFL learning significantly influences the development of knowledge acquisition and knowledge application, which are essential in a range of areas in education and society today.
COVID-19 Paradox: The Role of Privacy Concerns and Ad Intrusiveness on Consumer’s Attitude Toward App Usage Behavior
Authors: Bano Sobia , Sarfraz Usama , Salameh Anas A. , Jan Amin
Published: May 2022

The COVID-19 pandemic has changed lives in an unprecedented way. The most notable and urgent requirement to combat the epidemic was to transform the way human interacts with each other. The adherence to maintaining social distance has given an upsurge to the increased usage of mobile app users. This change in human interaction for fulling their basic to social to work needs through the intervention of app usage has led to privacy concerns by users. By keeping in view the changing dynamics of the way society works, this study is an endeavor to investigate gender differences of ad intrusive and privacy concerns on app usage behavior. Employing a quantitative research design, 371 respondents were surveyed using through an online structured questionnaire. Data were analyzed by using partial least square structural equation modeling (PLS-SEM). Results suggest that advertising intrusiveness and privacy concerns are significant in determining the consumer’s attitude toward App usage, and a positive attitude toward App usage results in App usage behavior. However, gender’s moderating role in attitude toward app usage and app usage behavior is insignificant for this study. The study provides a more comprehensive understanding and complements prior insights on ads intrusiveness and privacy concerns toward app usage.
Consumer Anxiety and Assertive Advertisement Preference: The Mediating Effect of Cognitive Fluency
Authors: Wang Jun
Published: May 2022

Companies often seek to persuade consumers to buy products or services through assertive advertising, but such advertising is often resisted by consumers. In order to identify ways to increase consumers' preference for assertive advertising, this study starts by considering consumers' anxiety and finds, through two between-group experiments, that the emotional state of consumers when viewing advertisements affects their attitudes toward assertive advertisements: anxious consumers have a more positive attitudes toward assertive advertisement, and cognitive fluency plays a mediating role in the relationship between consumer anxiety and consumer attitudes toward assertive advertisement. This study incorporates consumer anxiety into the study of assertive advertising, thus both enriching the theoretical research on assertive advertising and consumer anxiety and providing novel ideas for companies to enhance the effectiveness of their assertive advertising strategies.
Time After Time: Attachment Orientations and Impression Formation in Initial and Longer-Term Team Interactions
Authors: Gruda Dritjon , Berrios Raul Antonio , Kafetsios Konstantinos G. , McCleskey Jim Allen
Published: May 2022

If securely attached individuals typically exhibit more desirable attributes, can insecure individuals be perceived positively when working in teams despite their interpersonal disadvantages? In an exploratory study, using both a vignette based experimental research design (n = 636) and a round-robin study of professionals working on a team task for nine consecutive weeks (k = 648), we examined the evolving impressions of insecurely attached individuals over time. We find that while anxiously attached individuals are perceived more positively in initial interactions, this initial positive effect for anxious attachment disappeared over time as individuals within teams gained more relational knowledge about their team members. We also found a stable and negative effect of avoidant attachment. We discuss possible reasons for the temporal underpinnings of this effect and compare our findings to previous literature.
Unveiling the Effects of Stressors on Task Performance: The Role of Thriving at Work and Resilience
Authors: Hussain Zahid , Farid Hasan , Liu Xinran , Abbass Shaheen Wasim
Published: May 2022

This study unveils the effects of stressors on employees' task performance through the mediating role of thriving at work (TAW) and a moderating role of resilience (RES) grounding on conservation of resources (COR) theory. The analysis of collected data from 331 supervisor-employee dyads in the hospitality sector of China explicates that the role conflict (RC) and perceived workload have a negative influence on TAW, and thriving has a positive relationship with task performance. The results corroborate the mediating role of TAW between RC, perceived workload, and task performance. Furthermore, the RES suppressed the negative relationship between RC, perceived workload, and TAW. Moreover, our study underscores the theoretical and practical contributions regarding the negative influence of stressors on TAW by exhibiting the importance of the COR mechanism for employees' behavioral outcomes.
Chinese English as Foreign Language Teachers’ Self-Efficacy and Motivation as Predictors of Burnout
Authors: Song Meiling
Published: May 2022

In contemporary education, educators are a central and focal component of academic structures accountable for education growth. Educators have an effective role in presenting and planning an effective and important academic program. However, they usually experience burnout due to their stressful job that affects the procedure of their teaching. So, considering the variables that help them in mitigating their burnout should be taken into account. The concept such as educator efficacy has recently attracted significant attention in education fields which by itself emphasizes educators and gets them into the focus of attention in education. Also, the concept of motivation has a strong relationship with the expert aspect of the educators and its significance for language educators and their students are increasingly gaining respect in various worldwide settings, in which lower educators’ motivation is generally a reason for distress. To focus on the predictability role of these constructs on teachers’ burnout, 428 female and male Chinese English as Foreign Language (EFL) teachers from more than 17 cities in nine provinces of China were asked to fill out the three scales, namely, teacher motivation, efficacy, and burnout. The main results of the study, achieved through Pearson Correlation, postulate that the relationships exist among the variables of the study, and by running multiple regression analysis, it is revealed that both variables, namely, self-efficacy and motivation, were the predictors of teachers’ burnout, while self-efficacy was a better predictor. Regarding the applications, educator training plans can focus more on educator self-efficacy and motivation, because of their proven important role in decreasing educator burnout.
Knowledge, Attitude and Practice Toward COVID-19: A Cross-Sectional Study of Staff in China-Guinea Friendship Hospital, Guinea
Authors: Ji Wenyan , Camara M'Bemba Abdoulaye , Xia Jinggang , Ma Xiaobo , Chen Xintong , Liu Ying
Published: May 2022

Background

The purpose of this study was to assess the level of knowledge, attitude and practice of COVID-19 among staff in China-Guinea Friendship Hospital, and to confirm the effect of nosocomial infection management.

Methods

This cross-sectional study was conducted in December 2021. Information on socio demographic data, knowledge, attitude and practices related to COVID-19 was collected through a self-administered questionnaire.

Results

A total of 143 employees participated in the survey, with a response rate of 99.31% and a vaccination rate of 95.10%. The average knowledge score of COVID-19 was 8.39 ± 1.3 points (10 points in total), without significant differences between subgroups with different demographic variables (P > 0.05); more than 80% of the participants had a positive attitude, and 72.03–93.01% of the participants could take appropriate preventive practices in different environments such as hospital, outdoor or home.

Conclusion

The staff of the China-Guinea Friendship Hospital has good knowledge of COVID-19, a positive attitude and appropriate preventive practices. It can be concluded that the current nosocomial infection management is active and effective. Therefore, this study suggests that comprehensive activities such as training, promotion and supervision of COVID-19-related knowledge and countermeasures should be widely and continuously implemented in healthcare facilities, which will continuously improve the overall KAP level of hospital staff and play an important role in curbing the COVID-19 pandemic.
Synesthesia—Are All Mondays Blue?
Authors: Zelazny Aurore , Sørensen Thomas Alrik
Published: May 2022

Does Monday have a specific color? What does the ABC song taste like? These may sound like strange questions, but for a small number of people, such questions may be very reasonable. This is due to a phenomenon called synesthesia. It originates from Greek, meaning “joined” (syn) and “sensations” (esthesia). We experience the environment through a range of senses. You may hear music with your ears or enjoy the color of a clear sky with your eyes. However, when someone has synesthesia, certain experiences seem to have an additional sensory dimension. So, on top of hearing music, a person with synesthesia may also see the song in front of them. The phenomenon is still not fully understood. However, there are several suggestions about its mechanisms. Synesthesia provides an insight into individual differences in how we experience the world we live in.
How Does Cultural Burning Impact Biodiversity?
Authors: Hoffman Kira M. , Christianson Amy Cardinal , Davis Emma L. , Wickham Sara B. , Trant Andrew J.
Published: May 2022

Biodiversity is all the different types of life that are found in an area and it plays an important role in keeping ecosystems healthy. Unfortunately, biodiversity is decreasing around the world. Many species of plants and animals are rare and found only in certain ecosystems, which require disturbances, like fire, to stay healthy. Indigenous peoples have used fire as a tool to manage ecosystems for millions of years. This is called cultural burning. To understand how cultural burning impacts biodiversity, our research team conducted a review of over 1,000 scientific papers published globally from 1900 to 2020 (120 years). We assessed where, when, how, and why cultural burning was used to increase or decrease the numbers of certain animals, plants, insects, and even microbes! When cultural burning is used regularly, and under the right conditions, it can support and increase biodiversity and ecosystem health worldwide.
Research on Portal Venous Hemodynamics and Influencing Factors of Portal Vein System Thrombosis for Wilson’s Disease after Splenectomy
Authors: Zheng Zhou , Yu Qingsheng , Peng Hui , Zhang Wanzong , Shen Yi , Feng Hui , Huang Long , Zhou Fuhai , Zhang Qi , Wang Qin
Published: May 2022

Objective

Splenectomy is one crucial solution for hypersplenism with portal hypertension. However, portal vein system thrombosis (PVST) caused by hemodynamic changes affects the prognosis of patients. We analyze the changes in portal vein hemodynamics following splenectomy for Wilson’s disease combined with portal hypertension and the influencing factors that lead to PVST.

Methods

A retrospective cohort study was conducted, in which 237 Wilson’s disease patients with hypersplenism underwent splenectomy. The hemodynamic indices of the portal vein were monitored before surgery and on the 1st, 7th, and 14th days around surgery. The patients were divided into PVST and non-PVST groups. The clinical factors were identified by univariate and multivariate logistic regression. The Logit P was calculated according to the logistic regression prediction model, and the ROC curve for each independent factor was plotted.

Results

The portal vein velocity, flow, and inner diameter showed a downward trend around surgery, with statistically significant differences between each time point (P < 0.01). The PVST incidence rate was 55.7%. Univariate analysis revealed that the platelet (PLT) levels on the postoperative 3rd and 7th days (P = 0.001; P < 0.001), D-dimer (D-D) on the postoperative 7th and 14th days (P = 0.002; P < 0.001), preoperative portal vein velocity, flow, diameter (P < 0.001), and splenic vein diameter (P < 0.001) were all statistically and significantly different between the two groups. Multivariate logistic regression revealed a significant increase in PLT on the postoperative 7th day (OR = 1.043, 95% CI, 1.027–1.060, P < 0.001) and D-D on the postoperative 14th day (OR = 1.846, 95% CI, 1.400–2.435, P < 0.001). Preoperative portal and splenic vein diameters (OR = 1.565, 95% CI, 1.213–2.019, P = 0.001; OR = 1.671, 95% CI, 1.305–2.140, P < 0.001) were the risk factors for PVST. However, preoperative portal vein velocity and flow (OR = 0.578, 95% CI, 0.409–0.818, P = 0.002; OR = 0.987, 95% CI, 0.975–0.990, P = 0.046) were protective factors for PVST. Logit P was calculated using a logistic regression prediction model with a cut-off value of −0.32 and an area under receiver operating characteristic curve of 0.952 with 88.61% accuracy.

Conclusions

Splenectomy relieves portal hypertension by reducing the hemodynamics index. PVST is linked to multiple factors, including preoperative portal vein diameter, velocity, flow, and splenic vein diameter, especially PLT on the postoperative 7th day and D-D on the postoperative 14th day. The predictive model is accurate in predicting PVST.
Optic Pathway–Hypothalamic Glioma Apoplexy: A Report of Two Cases and Systematic Review of the Literature
Authors: Baeesa Saleh , Maghrabi Yazid , Moshref Rana , Al-Maghrabi Jaudah
Published: May 2022

Background

Hemorrhage into optic pathway–hypothalamic glioma (OPHG) is rare. Variable clinical presentations and outcomes are associated with such pathology. We aim to present two infants presented with OPHG and a systematic review of the literature.

Methods

We describe two cases of infants presenting with sudden decreased vision, poor feeding, and irritability due to OPHG. Both patients underwent urgent craniotomy and subtotal resection followed by chemotherapy. We systematically reviewed the literature using PubMed, Google Scholar, and Embase. In addition, we included all English published reports for all ages discussing the optic pathway (optic nerve and optic chiasm) or hypothalamic glioma associated with hemorrhage from the year of the first reported case (1970) to January 2022.

Results

Of 17,949, 44 articles met the inclusion criteria of this review. A total of 56 cases were described with a mean of 21.35 years (0.5–70), with the male gender 52% and the female gender 45%. The hemorrhage location was sellar/suprasellar in 43% cases. Histopathology of included cases was pilocytic astrocytoma in 41%, followed by pilomyxoid astrocytoma in 16% cases. The outcome was unfavorable; 37.5% cases showed improvement, whereas 18% cases resulted in death.

Conclusion

Apoplexy of the OPHG can be fatal and associated with poor outcomes. A systematic review of the literature has shown that younger age, pilocytic or pilomexyoid astrocytoma histopathology, and chiasmal/hypothalamic locations are associated with a higher risk of intertumoral hemorrhage and poor prognosis. Further genetic studies for OPHG may provide information for high-risk patients.
Multi-Omics Integrative Analysis Coupled to Control Theory and Computational Simulation of a Genome-Scale metabolic Model Reveal Controlling Biological Switches in Human Astrocytes Under Palmitic Acid-Induced Lipotoxicity
Authors: Angarita-Rodríguez Andrea , Mendoza-Mejía Nicolás , Gonzalez Janneth , Aristizabal Andrés Felipe , Hidalgo-Lanussa Oscar , Rubio-Mesa Juan J. , Barreto George E. , Pinzon Andres
Published: May 2022

Astrocytes play an important role in various processes in the brain, including pathological conditions such as neurodegenerative diseases. Recent studies have shown that the increase in saturated fatty acids such as palmitic acid (PA) triggers pro-inflammatory pathways in the brain. The use of synthetic neurosteroids such as tibolone has demonstrated neuro-protective mechanisms. However, broad studies, with a systemic point of view on the neurodegenerative role of PA and the neuro-protective mechanisms of tibolone are lacking. In this study, we performed the integration of multi-omic data (transcriptome and proteome) into a human astrocyte genomic scale metabolic model to study the astrocytic response during palmitate treatment. We evaluated metabolic fluxes in three scenarios (healthy, induced inflammation by PA, and tibolone treatment under PA inflammation). We also applied a control theory approach to identify those reactions that exert more control in the astrocytic system. Our results suggest that PA generates a modulation of central and secondary metabolism, showing a switch in energy source use through inhibition of folate cycle and fatty acid β-oxidation and upregulation of ketone bodies formation. We found 25 metabolic switches under PA-mediated cellular regulation, 9 of which were critical only in the inflammatory scenario but not in the protective tibolone one. Within these reactions, inhibitory, total, and directional coupling profiles were key findings, playing a fundamental role in the (de)regulation in metabolic pathways that may increase neurotoxicity and represent potential treatment targets. Finally, the overall framework of our approach facilitates the understanding of complex metabolic regulation, and it can be used for in silico exploration of the mechanisms of astrocytic cell regulation, directing a more complex future experimental work in neurodegenerative diseases.
Diversity Chromosome Evolution of Ty1-copia Retrotransposons in Pennisetum purpureum Revealed by FISH
Authors: Yu Zehuai , Huang Yongji , Wu Jiayun , Zhang Muqing , Deng Zuhu
Published: May 2022

Pennisetum purpureum is a potential species for biofuel production. Characterization and chromosomal distribution of retrotransposons could enhance the comprehension of the role and dynamics of the repetitive elements in plants. In this study, a phylogenetic tree was constructed according to the conserved reverse transcriptase sequences and revealed that these Ty1-copia retrotransposons had a typical structure. Analysis showed that the total Ty1-copia retrotransposons had a significant component, as high as 5.12 × 103 copy numbers in P. purpureum. Then, the chromosomal pattern of four known lineages were also analyzed with the Pennisetum glaucum genome, which suggested that the Sire/Maximus lineage had the highest copy number and followed by Tork/Angela, Tork/TAR, Retrofit/Ale. Additionally, the chromosomal distribution of total Ty1-copia retrotransposons was detected by fluorescence in situ hybridization (FISH) to be a dispersed pattern with weak clustering, mostly near the centromeric regions of P. purpureum chromosomes; interestingly, there were four obvious signals in the subterminal chromosomes. These results suggested that there occurred differential dynamic evolution directions of Ty1-copia retrotransposons within P. purpureum. Furthermore, co-localization of Ty1-copia, 5S rDNA, and 35S rDNA indicated that two chromosome 2 and four chromosome 4 were identified. Concurrently, subterminal signals of Ty1-copia-type retrotransposons were located on four other homologous chromosomes. Altogether, these results shed light on the diversification of Ty1-copia retrotransposons and have the significance for generation of valid chromosomal markers in retrotransposon families.
Mineral Composition of Soil and the Wheat Grain in Intensive and Conservation Cropping Systems
Authors: Dolijanović Željko , Nikolić Svetlana Roljević , Dragicevic Vesna , Mutić Jelena , Šeremešić Srđan , Jovović Zoran , Popović Djordjević Jelena
Published: May 2022

Tillage methods and intensity can be used as means of improving macronutrient and trace element concentration in soil and crops. A two-year field experiment was conducted to examine the impact of two cropping systems—intensive (ICS) and conservation (CCS), on the macro- and microelements in the soil and their accumulation in the grain of two cultivars of winter wheat. The experiment was conducted in a randomized complete block design with three replications of each tillage treatment. The results showed that the content of available N (0.7 kg ha−1) and organic matter (0.04%) slightly increased in CCS compared to ICS. The concentrations of Ca, K, and S macroelements and microelements such as Ba, Cr, Hg, and Sr in the soil were significantly higher in CCS than in ICS. Higher concentrations of macroelements K and P, microelements such as Fe, Zn, and As, and the greater value of the bioaccumulation factor for elements essential to humans such as P, Cu, Fe, and Zn, were also found in CCS compared to ICS. On the other hand, wheat cultivars grown in ICS were more efficient at accumulating macroelements and some trace elements than ones grown in CCS. While it is not without challenges, the conservation cropping system could represent an important part of the long-term strategy to sustainably improve soil fertility and the nutritional quality of the wheat grain.
Cultivation of Two Barnyard Varieties Improves Physicochemical Properties of Saline-Alkali Land through Mediating Rhizospheric Microbiome and Metabolome
Authors: Wang Xueqin , Xu Xing , Lu Anqiao , Li Xin , Zhang Fengju
Published: May 2022

The present study was conducted to compare the effect of two barnyard millet varieties viz. Echinochloa frumentacea (L.) (HNJZ) and Echinochloa crusgalli (L.) var. austro-japonensis (BZ), on fertility and physicochemical properties of alkaline soil of Ningxia, China. Soil rhizosphere of BZ and HNJZ with three replicates (5 plants from each replicate) were collected for bacterial communities metagenomic profiling and six rhizosphere soil samples from each treatment for untargeted-metabolomic analysis. Results revealed significant (p < 0.05) improvement in soil fertility for both millet varieties at 0–20 cm depth than 20–40 cm by decreasing the alkalinity and pH, while increasing the organic matter. Proteobacteria, Firmicutes, and Actinobacteria were the most abundant phyla, whereas Megamonas, uncharacterized_Acidobacteria, and Halomonas were the most abundant genera. No difference in bacterial alpha diversity parameters was observed between HNJZ and BZ rhizospheres. Relative abundance of Cellvibrio and Devosia was increased (p < 0.05) in HNJZ, while Arenimonas, Gillisia, Sphingomonas, uncharacterized_Gammaproteobacteria, and Lysobacter were increased significantly (p < 0.05) in BZ rhizospheres. Beta diversity analysis revealed more diverse bacterial communities structure in HNJZ rhizosphere with higher Firmicutes ratio. Non-targeted metabolomic analysis revealed biosynthesis of alkaloids, histamine H2/H3 receptor agonists/antagonists, and arginine/proline metabolism as top three enriched KEGG pathways. The present study indicated that both millet varieties contributed to the reclamation of saline-alkali soil through reducing pH, total salts, and alkalinity while increasing the organic matter.
Assessing Biosecurity Compliance in Poultry Farms: A Survey in a Densely Populated Poultry Area in North East Italy
Authors: Tilli Giuditta , Laconi Andrea , Galuppo Francesco , Mughini-Gras Lapo , Piccirillo Alessandra
Published: May 2022

Biosecurity in poultry farms represents the first line of defense against the entry and spread of pathogens that may have animal health, food safety, and economic consequences. The aim of this study was to assess biosecurity compliance in poultry farms located in a densely populated poultry area in North East Italy. A total of 259 poultry farms (i.e., broilers, turkeys, and layers) were surveyed between 2018 and 2019 using standardized checklists, and differences in biosecurity compliance between the poultry sectors and years (only for turkey farms) were tested for significance. Among the three sectors, turkey farms showed the highest compliance. Farm hygiene, infrastructure condition, cleaning and disinfection tools, and procedures were the biosecurity measures most complied with. Some deficiencies were observed in the cleanliness of the farm hygiene lock in broiler farms, as well as the presence of the house hygiene lock in broiler and layer farms and an adequate coverage of built-up litter in turkey and broiler farms. In conclusion, this study highlighted a generally high level of biosecurity in the visited poultry farms (probably due to the stringent national regulation and the integration of the poultry industry) and identified some measures that still need to be improved.
The Rapid Emergence of Ceftazidime-Avibactam Resistance Mediated by KPC Variants in Carbapenem-Resistant Klebsiella pneumoniae in Zhejiang Province, China
Authors: Liu Congcong , Wu Yuchen , Huang Ling , Zhang Yanyan , Sun Qiaoling , Lu Jiayue , Zeng Yu , Dong Ning , Cai Chang , Shen Zhangqi , Chen Gongxiang , Zhang Rong
Published: May 2022

Ceftazidime-avibactam (CAV) is a new treatment option against carbapenem-resistant Klebsiella pneumoniae (CRKP) infections. However, the rapid emergence of CAV resistance mediated by KPC variants has posed a severe threat to healthcare after its clinical application. The characteristics of CAV resistance in CRKP strains needs to be determined in China. A total of 477 CRKP isolates were collected from 46 hospitals in Zhejiang Province from 2018 to 2021. The results demonstrated that CAV had a potent activity against 94.5% of all CRKP (451/477, 95% CI: 93.0–96.1%) and 86.0% of CRKP strains carrying blaKPC genes (410/477, 95% CI: 83.5–88.4%). A total of 26 CAV-resistant strains were found. Among these strains, sixteen harbored metallo-β lactamases, and two carried KPC-2 carbapenemase and mutated ompK35 and ompK36. Eight CRKP strains encoded KPC-33 or KPC-93, belonging to ST11, among which seven strains were detected in patients hospitalized in 2021 after exposure to CAV and one strain was associated with intra-hospital spread. CAV is a potent agent in vitro against CRKP strains. The rapid development of CAV resistance mediated by various KPC variants after a short period of CAV treatment has increased and brought difficulties in treating infections caused by CRKP strains, especially those belonging to ST11. The surveillance of bacterial resistance against CAV is highly recommended due to the steep development of CAV resistance and rapid evolution of KPC enzymes.
Inherited Retinal Dystrophies: Role of Oxidative Stress and Inflammation in Their Physiopathology and Therapeutic Implications
Authors: Pinilla Isabel , Maneu Victoria , Campello Laura , Fernández-Sánchez Laura , Martínez-Gil Natalia , Kutsyr Oksana , Sánchez-Sáez Xavier , Sánchez-Castillo Carla , Lax Pedro , Cuenca Nicolás
Published: May 2022

Inherited retinal dystrophies (IRDs) are a large group of genetically and clinically heterogeneous diseases characterized by the progressive degeneration of the retina, ultimately leading to loss of visual function. Oxidative stress and inflammation play fundamental roles in the physiopathology of these diseases. Photoreceptor cell death induces an inflammatory state in the retina. The activation of several molecular pathways triggers different cellular responses to injury, including the activation of microglia to eliminate debris and recruit inflammatory cells from circulation. Therapeutical options for IRDs are currently limited, although a small number of patients have been successfully treated by gene therapy. Many other therapeutic strategies are being pursued to mitigate the deleterious effects of IRDs associated with oxidative metabolism and/or inflammation, including inhibiting reactive oxygen species’ accumulation and inflammatory responses, and blocking autophagy. Several compounds are being tested in clinical trials, generating great expectations for their implementation. The present review discusses the main death mechanisms that occur in IRDs and the latest therapies that are under investigation.