bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of non-genetic factors on first lactation and lifetime performance traits in Nili-Ravi buffaloes
Authors: Tamboli Pooja , Bharadwaj Anurag , Chaurasiya Amit Kumar , Jan Mustafa H. , Kumar Sanjay , Khanna Sudhir
Published: February 2023

The objective of this research was to evaluate the effects of various non-genetic factors on first lactation and lifetime productivity in Nili-Ravi buffaloes, including season of birth, period of birth, age at first calving, season of first calving, period of first calving, and number of lactations completed. The livestock data on first lactation and lifetime performance traits relevant to 501 Nili-Ravi buffaloes were collected from the history sheet of the animal for a period from 1983 to 2017 (i.e., 35 years) and maintained at the Indian Council of Agricultural Research—Central Institute for Research on Buffaloes (ICAR-CIRB) sub-campus, in Nabha, Punjab, India. To evaluate the least-squares means (LSMs) and the effect of non-genetic factors on performance traits, a least-squares analysis model was applied. The overall LSM for age at first calving (AFC) was 45.03 ± 0.40 months and ranged from 34 to 54 months. The results indicated that the effect of AFC was highly significant (p ≤ 0.01) on first lactation total milk yield (FLTMY), first lactation standard milk yield (305 days or less) (FLSMY), first peak milk yield (FPY), and first lactation length (FLL) in the Nili-Ravi breed of buffaloes. FLTMY, FLSMY, and FPY were highest (2,250.08 ± 48.16 kg, 1,944.68 ± 31.20 kg, and 9.32 ± 0.16 kg/day, respectively) for animals with an AFC of 42–48 months. Furthermore, FLTMY, FLSMY, and FLL were highest (2,411.02 ± 68.68 kg, 2,008.81 ± 44.49 kg, and 357.43 ± 8.13 days, respectively) in animals that first calved in the autumn season. However, the lowest first dry period (FDP), first service period (FSP), and first calving interval (FCI) (110.63 ± 7.42 days, 125.48 ± 9.04 days, and 443.63 ± 9.00 days, respectively) were found for animals that first calved in the rainy season. The overall LSM for herd life (HL), productive life (PL), productive days (PDs), unproductive days (UDs), total lifetime milk yield (total LTMY), standard lifetime milk yield (standard LTMY), milk yield per day of productive life (MY/PL), milk yield per day of productive days (MY/PDs), and milk yield per day of herd life (MY/HL) were estimated as 3,779.84 ± 31.86 days, 2,078.55 ± 24.32 days, 1,552.74 ± 20.06 days, 525.81 ± 12.44 days, 10,229.71 ± 195.31 kg, 9,203.64 ± 173.52 kg, 4.86 ± 0.08 kg/day, 6.46 ± 0.08 kg/day, and 2.66 ± 0.04 kg/day, respectively. The effect of AFC on HL, PDs, UDs, total LTMY, and standard LTMY was highly significant (p ≤ 0.01). Furthermore, the effect of season of first calving on HL, PL, and PDs was significant (p ≤ 0.05). In addition, the effect of the period of first calving on HL, PDs, standard LTMY, and total LTMY was significant (p ≤ 0.05). In these contexts, it can be concluded that the buffaloes of the Nili-Ravi breed with an AFC of 42–48 months performed better than animals with a later AFC in terms of production, reproduction, and lifetime traits.
The applicability of the European GMO legislation to epigenetically modified organisms
Authors: Faltus Timo
Published: February 2023

In addition to classic genetic engineering for the targeted modification of the base sequence of the DNA, epigenetic methods for the targeted modification of the genetic material without base changes are increasingly being used. Such epigenetic techniques can be used, for example, to influence stress tolerance to heat or aridity in plants. The regulatory handling of organisms generated by means of epigenetic techniques on the grounds of genetic engineering law has not yet been clarified. This paper critically reviews the legal classification of epigenetically modified organisms as GMOs as expressed in the study on New Genomic Techniques published in April 2021 by the European Commission. The paper shows that there are reasons to assume that epigenetically modified organisms are not covered by the European GMO legislation. In addition, the paper provides an introductory overview of the significance of epigenetics and the methods used to intentionally influence epigenetic traits and illustrates the possibility for a consistent, risk-based regulation of epigenetic modifications.
Role of amino acid metabolism in mitochondrial homeostasis
Authors: Li Qiaochu , Hoppe Thorsten
Published: February 2023

Mitochondria are central hubs for energy production, metabolism and cellular signal transduction in eukaryotic cells. Maintenance of mitochondrial homeostasis is important for cellular function and survival. In particular, cellular metabolic state is in constant communication with mitochondrial homeostasis. One of the most important metabolic processes that provide energy in the cell is amino acid metabolism. Almost all of the 20 amino acids that serve as the building blocks of proteins are produced or degraded in the mitochondria. The synthesis of the amino acids aspartate and arginine depends on the activity of the respiratory chain, which is essential for cell proliferation. The degradation of branched-chain amino acids mainly occurs in the mitochondrial matrix, contributing to energy metabolism, mitochondrial biogenesis, as well as protein quality control in both mitochondria and cytosol. Dietary supplementation or restriction of amino acids in worms, flies and mice modulates lifespan and health, which has been associated with changes in mitochondrial biogenesis, antioxidant response, as well as the activity of tricarboxylic acid cycle and respiratory chain. Consequently, impaired amino acid metabolism has been associated with both primary mitochondrial diseases and diseases with mitochondrial dysfunction such as cancer. Here, we present recent observations on the crosstalk between amino acid metabolism and mitochondrial homeostasis, summarise the underlying molecular mechanisms to date, and discuss their role in cellular functions and organismal physiology.
Going viral: A science communication collaboration in the era of COVID-19 and social media
Authors: Wiles Siouxsie , Morris Toby , Priestley Rebecca
Published: February 2023

On 9 March 2020, 2 days before the World Health Organization declared COVID-19 a global pandemic, two of the authors (microbiologist and infectious diseases expert Associate Professor Siouxsie Wiles and cartoonist Toby Morris) released their first output together: an animated GIF (Graphics Interchange Format) known as “Flatten the Curve”. The graphic went viral on Twitter with over 10 million impressions in 3 days. Flatten the Curve was the first of more than 70 graphics produced by our collaboration, all designed as accessible visual communication about COVID-19. The graphics, all released under a Creative Commons CC-BY-SA-4.0 license, have been translated into multiple languages, used by communities, politicians, and public health officials around the world, and the collaborators have won multiple awards for their work.
Competitive perimeter defense with a turret and a mobile vehicle
Authors: Bajaj Shivam , Bopardikar Shaunak D. , Moll Alexander Von , Torng Eric , Casbeer David W.
Published: February 2023

We consider perimeter defense problem in a planar conical environment with two cooperative heterogeneous defenders, i.e., a turret and a mobile vehicle, that seek to defend a concentric perimeter against mobile intruders. Arbitrary numbers of intruders are released at the circumference of the environment at arbitrary time instants and locations. Upon release, they move radially inwards with fixed speed towards the perimeter. The defenders are heterogeneous in terms of their motion and capture capabilities. Specifically, the turret has a finite engagement range and can only turn (clockwise or anti-clockwise) in the environment with fixed angular rate whereas, the vehicle has a finite capture radius and can move in any direction with unit speed. We present a competitive analysis approach to this perimeter defense problem by measuring the performance of multiple cooperative online algorithms for the defenders against arbitrary inputs, relative to an optimal offline algorithm that has information about the entire input sequence in advance. Specifically, we establish necessary conditions on the parameter space to guarantee finite competitiveness of any online algorithm. We then design and analyze four cooperative online algorithms and characterize parameter regimes in which they have finite competitive ratios. In particular, our first two algorithms are 1-competitive in specific parameter regimes, our third algorithm exhibits different competitive ratios in different regimes of problem parameters, and our fourth algorithm is 1.5-competitive in specific parameter regimes. Finally, we provide multiple numerical plots in the parameter space to reveal additional insights into the relative performance of our algorithms.
Migration law for random parameters rockfall in steeply dipping coal seams
Authors: Liu Ming , Chen Jie , Xiao Yang , Lv Wenyu
Published: February 2023

The migration process of rockfall has certain complexity and randomness in steeply dipping coal seams (SDCS). The three-dimensional random migration of rockfall with random parameters cannot be accurately simulated. Taking the transport process of arbitrarily shaped rockfall formed by roof leakage in steep seam working face as the research object, a method for generating rockfall with the random shape of irregular polyhedrons based on the ellipsoid equation is provided. And based on the geographic information system data, such as the contour line of the bottom floor of the working face, the 3D grid model of the bottom floor is established. The random transport process of rockfall in three-dimensional mining space was simulated using Monte Carlo random simulation technology combined with the energy tracking method (ETM) to compile the program, considering the randomness of collision recovery coefficient and friction coefficient between rockfall and working face floor. The influence of randomness of parameters on the migration velocity, angular velocity and energy of rockfall is analyzed. With the increase of the coefficient of variation of parameters, the influence of the randomness of the parameters on the transport characteristics of rockfall becomes greater. The impact of the collision recovery coefficient on the migration process of rockfall is much more significant than that of the friction coefficient. The offset ratio of rockfall increases with the increase of the variation coefficient. The method presented in this paper can be used to simulate the motion of rockfall more accurately, and a theoretical basis is provided for predicting and protecting rockfall hazards.
Precarity in the lives of contract teachers: A qualitative study from Odisha, India
Authors: Apat Biswajit , Swain Pranaya
Published: February 2023

School enrollment rates in developing countries have increased substantially over the past few decades. However, due largely to budget constraints, hiring contract teachers has become an ad hoc, yet a popular solution to teacher shortages in Asia, Africa, and Latin America. Studies concerning contract teachers have primarily focused on their performance, efficiency, and cost-effectiveness. In light of the literature on precarity associated with contractual employment, this article seeks to explore how contractual employment affects teachers in India. The study analyzes narrative data obtained through semistructured interviews with 17 contract teachers employed in government-run schools in Odisha, a state in eastern India. According to thematic analysis of the data, participants experience precarity in six dimensions: prioritisation of non-teaching work over teaching, financial hardships, sense of inferiority, anxiety about transfer, experiences of discrimination and desire for course correction. We argue that these six dimensions contribute to the demoralization and disempowerment of teachers. We also explore possible explanations for why Odisha continues to employ contract teachers despite criticism. It is recommended that policymakers be sensitized to the plight of contract teachers and reconsider the policy of contractual employment.
Effectiveness of the Natura 2000 network for freshwater fish conservation in a Mediterranean region
Authors: Gavioli Anna , Filipe Ana Filipa , Patonai Katalin , Milardi Marco , Castaldelli Giuseppe
Published: February 2023

Protected areas (PAs) are the cornerstones of global biodiversity conservation efforts, but to fulfil this role they must be effective at conserving both habitat and species. Among protected taxa, freshwater fish are exposed to multiple disturbances and are considered one of the most endangered. The Natura 2000 reserves network was established with the aim of preserving biodiversity across Europe, but few assessments have been made on its effectiveness on the conservation of freshwater fish species. We tested the hypothesis that fish community is exposed to less anthropogenic pressures within the Natura 2000 sites than outside, hosting a higher number of native species and maintain lower number of non-native species. We tested these hypotheses considering 3,777 sampling sites, found across the entire Italian territory. Results showed that PAs did not guarantee less anthropogenic impacts and higher fish species richness than outside PAs, suggesting that PAs are not a panacea for anthropogenic pressures and safeguarding fish diversity. Nevertheless, more caution should be applied to the management measures and the design of new PAs due to the limitations of the protection of a single stretch within a whole river ecosystem. Moreover, the impossibility to operate any management of invasive fish species on the broad scale of a whole river basin is likely the most limiting factor to fish biodiversity conservation in Italy. Finally, it is also necessary to extend the analysis to other basins and Natura 2000 sites in Europe.
Particulate organic carbon in the deep-water region of the Gulf of Mexico
Authors: Contreras-Pacheco Yéssica Vanessa , Herzka Sharon Z. , Vallejo-Espinosa Gerardo , Herguera Juan Carlos
Published: February 2023

Ocean eddies play a major role in lateral and vertical mixing processes of particulate organic carbon (POC), as well as in the transport of heat, salinity, and biogeochemical tracers. In the open waters of the Gulf of Mexico (GoM), however, there are limited observations to characterize how these mesoscale structures affect the spatial distribution of POC in the upper water column, which is important for organic matter cycling and export. We present the distribution patterns of POC relative to mesoscale features throughout the water column in the deep-water region of the GoM during three oceanographic cruises held during the summer months of 2015, 2016, and 2017. Samples were collected under well-stratified upper ocean conditions, which allowed us to assess the spatial and temporal distribution of POC as a function of non-steric sea surface height, density, apparent oxygen utilization, and chlorophyll fluorescence. We further explored the variability of integrated surface layer POC concentrations at stations located within the cores and the edges of cyclonic and anticyclonic eddies, and those collected outside these structures. Although our results indicate mesoscale eddies modulate several important physical and biogeochemical variables and POC concentrations in the upper ocean, these features do not fully explain the spatial distribution of POC concentrations throughout the deep-water region of the GoM. Relatively lower POC concentrations were observed in the border of the cyclonic and the center of the anticyclonic eddies, in contrast to the relatively higher POC concentrations at the center of the cyclonic and the border of anticyclonic eddies. We observed high variability in POC concentration variability outside mesoscale structures, which may be attributed to other processes such as upwelling over the shelves, and the contribution by rivers during the summer especially in the northern and southern GoM.
Fabrication of novel oxochalcogens halides of manganese and tin nanocomposites as highly efficient photocatalysts for dye degradation and excellent antimicrobial activity
Authors: Umar Misbah , Mansoor Sana , Javed Mohsin , Hussain Nadia , Bajaber Majed A. , Iqbal Shahid , Alhujaily Ahmad , Mohyuddin Ayesha , Ajaz Humayun , Rauf Abdul , Bahadur Ali , Al-Fawzan Foziah F. , Elkaeed Eslam B.
Published: February 2023

The dark brown and white crystals of manganese and tin (Mn2Se3Cl2O7 and SnSe3O4Cl) have been synthesized by solid-state reaction at 450 C. The morphology and the elemental analysis of newly synthesized compounds were studied by SEM and EDX Analysis. SEM analysis reveals that the particle size for Mn2Se3Cl2O7 was found to be 0.2–2.5 μm and for SnSe3O4Cl 2.0–6.0 μm. The EDX studies showed the presence of Mn, Se, O, Cl, and Sn elements. Powdered XRD confirmed the presence of a new phase present in these compounds. Under UV-vis irradiation, the kinetics of methylene blue (MB) degradation catalyzed by produced nanoparticles were monitored. The dye degradation efficiency was estimated, and results reveals that after 150 min of irradiation, almost 75% of the dye was degraded in the presence of Mn compound while 71% degradation was shown by Sn compound. Both composites display antimicrobial activity against Staphylococcus aureus and Escherichia coli with a maximum value of 34.5 mm. The maximum antimicrobial activity shown by Mn-incorporated nanocomposites estimated at 32.5 mm was against Gram-positive bacteria and 26.4 mm against Gram-negative bacteria. Similarly, the maximum antifungal activity shown by Sn incorporated estimated at 33.9 mm was compared to Gram-positive bacteria and 27.8 mm against Gram-negative bacteria.
Impact of biochar on the fungal decay resistance of hemp-polylactic acid composites against wood basidiomycetes
Authors: Zouari Mariem , Mikuljan Marica , Schwarzkopf Matthew
Published: February 2023

Natural fibers reinforced composites represent a sustainable alternative to synthetic polymer-based materials. However, natural fibers are susceptible to fungal degradation which reduces the lifespan of the composites in use. The objective of this study was to investigate the effect of biochar (BC) on improving the resistance to fungal decay of polylactic acid (PLA) and hemp-PLA composites. The influence of incremental BC loadings (0, 5, 10, and 20 wt%) on physiological and morphological properties of the composites was evaluated. Weight loss, color stability, microscopic observations, and water contact angle measurements were performed during and after 5-months of exposure to white (Trametes Versicolor) and brown (Fomitopsis Betulina) rot fungi. We found that the addition of 10 wt% BC decreased the total weight loss in PLA and hemp-PLA composites by 93% and 34%, respectively in the case of exposure to white rot and by 66% and 83%, respectively in the case of exposure to brown rot as compared to neat PLA. BC addition improved the color stability of the composites. The color change in hemp-PLA samples loaded by 10 wt% of BC decreased by 44% and 37%, in the case of exposure to white and brown rots, respectively as compared to hemp-PLA reference. Moreover, samples containing BC (5 wt% and 10 wt%) had higher gloss after the decay test among all other samples. Micrographs revealed that white and brown rots invaded the surface of the composites at the beginning of the incubation period and then accessed the interior of the materials. Micro-cracks occurred in samples containing hemp fibers which enabled the fungi to invade the internal structure of the composites. At the same time, fungi grew on the surface of samples without hemp fibers except for PLA with 20% BC content which had a porous surface that allowed fungi to access the interior. The hydrophobicity of all samples increased during the first 3 months of the incubation and then decreased again by the end of the incubation as the surface of the composites started to be damaged. Our study provided a novel direction of BC as an organic additive in bio-composites. Uncovering the potential of BC in improving the resistance to fungal decay of natural fibers-reinforced composites paves the way for BC’s utilization as a renewable additive in various applications such as bio-based packaging materials.
Isolation, identification, and pathogenicity analysis of newly emerging gosling astrovirus in South China
Authors: Xu Jingyu , Gao Liguo , Zhu Puduo , Chen Sheng , Chen Zixian , Yan Zhuanqiang , Lin Wencheng , Yin Lijuan , Javed M. Tariq , Tang Zhaoxin , Chen Feng
Published: February 2023

Goose astroviruses (GoAstV) cause fatal gout and decrease product performance in the waterfowl industry across the world. Since no effective vaccines are available, studies on the epidemiology of the virus are necessary for vaccine development. In this study, we collected 94 gout samples from goose farms in the Guangdong Province of South China. Among them, 87 samples (92.6%) tested positive for GoAstV, out of which five GoAstV strains were isolated after four generations of blind transmission through healthy 13-day-old goose embryos. The whole genome of the isolates was sequenced and further analyzed by comparing the sequences with published sequences from China and other parts of the world. The results of the alignment analysis showed that nucleotide sequence similarities among the five GoAstV isolates were around 97.4–98.8%, 98.6–100%, 98.1–99.8%, and 96.7–100% for the whole genome, ORF1a, ORF1b, and ORF2, respectively. These results showed that the GoAstV isolates were highly similar to each other, although they were prevalent in five different regions of the Guangdong Province. The results of the phylogenetic analysis showed that the whole genome, along with the ORF1a, ORF1b, and ORF2 genes of the isolates, were clustered on a single branch, along with the recently published GoAstV-2, and were very distinct from the DNA sequences of the GoAstV-1 virus. In this study, we also reproduced the clinical symptoms of natural infection using the GoAstV-GD2101 isolates, confirming that the gout-causing pathogen in goslings was the goose astrovirus. These findings provided new insights into the pathogenicity and genetic evolution of GoAstV and laid the foundation for effectively controlling the disease.
Establishment of a murine model of congenital toxoplasmosis and validation of a qPCR assay to assess the parasite load in maternal and fetal tissues
Authors: Souza Jéssica S. , Farani Priscila S. G. , Ferreira Beatriz I. S. , Barbosa Helene S. , Menna-Barreto Rubem F. S. , Moreira Otacilio C. , Mariante Rafael M.
Published: February 2023

Toxoplasma gondii is the causative agent of toxoplasmosis, a disease that affects warm-blooded animals and one third of the human population worldwide. Pregnant women who have never been exposed to the parasite constitute an important risk group, as infection during pregnancy often leads to congenital toxoplasmosis, the most severe form of the disease. Current therapy for toxoplasmosis is the same as it was 50 years ago and has little or no effect when vertical transmission occurs. Therefore, it is urgent to develop new strategies to prevent mother-to-fetus transmission. The implementation of experimental animal models of congenital toxoplasmosis that reproduces the transmission rates and clinical signs in humans opens an avenue of possibilities to interfere in the progression of the disease. In addition, knowing the parasite load in maternal and fetal tissues after infection, which may be related to organ abnormalities and disease outcome, is another important step in designing a promising intervention strategy. Therefore, we implemented here a murine model of congenital toxoplasmosis with outbred Swiss Webster mice infected intravenously with tachyzoites of the ME49 strain of T. gondii that mimics the frequency of transmission of the parasite, as well as important clinical signs of human congenital toxoplasmosis, such as macrocephaly, in addition to providing a highly sensitive quantitative real-time PCR assay to assess parasite load in mouse tissues. As the disease is not restricted to humans, also affecting several domestic animals, including companion animals and livestock, they can also benefit from the model presented in this study.
Solid-state fermentation of Apocynum venetum L. by Aspergillus niger: Effect on phenolic compounds, antioxidant activities and metabolic syndrome-associated enzymes
Authors: Cao Cha , Lin Dengfan , Zhou Yingjun , Li Na , Wang Yiwen , Gong Wenbeng , Zhu Zuohua , Liu Chengwei , Yan Li , Hu Zhenxiu , Peng Yuande , Xie Chunliang
Published: February 2023

This study aimed to evaluate the effect of solid-state fermentation (SSF) with Aspergillus niger on the total phenolic content (TPC), the total flavonoid content (TFC), individual phenolic contents, and antioxidant and inhibitory activities against metabolic syndrome-associated enzymes in an ethanol extract from Apocynum venetum L. (AVL). TPC, TFC, and the contents of quercetin and kaempferol during SSF were 1.52-, 1.33-, 3.64-, and 2.22-fold higher than those of native AVL in the ethyl acetate (EA) subfraction of the ethanol extract. The ABTS·+, DPPH· scavenging, and inhibitory activities against α-glucosidase and pancreatic lipase were found to be highest in the EA subfraction. Fermentation significantly increased the ABTS radical cation, DPPH radical scavenging, and pancreatic lipase inhibitory activities by 1.33, 1.39, and 1.28 times, respectively. TPC showed a significantly positive correlation with antioxidant activities or inhibition against metabolic syndrome-associated enzymes. This study provides a theoretical basis for producing tea products with enhanced antioxidant, antidiabetic, and antihyperlipidemic activities.
A nomogram prediction model for lymph node metastasis risk after neoadjuvant chemoradiotherapy in rectal cancer patients based on SEER database
Authors: Liu Xiaoshuang , Sha Li , Huang Cheng , Kong Xiancheng , Yan Feihu , Shi Xiaohui , Tang Xuefeng
Published: February 2023

Background

Rectal cancer patients who received neoadjuvant chemoradiotherapy (CRT) may have a lower cancer stage and a better prognosis. Some patients may be able to avoid invasive surgery. It is critical to accurately assess lymph node metastases (LNM) after neoadjuvant chemoradiotherapy. The goal of this study is to identify clinical variables associated with LNM and to develop a nomogram for LNM prediction in rectal cancer patients following nCRT.

Methods

From 2010 to 2015, patients were drawn from the Surveillance, Epidemiology, and End Results (SEER) database. To identify clinical factors associated with LNM, the least absolute shrinkage and selection operator (LASSO) aggression and multivariate logistic regression analyses were used. To predict the likelihood of LNM, a nomogram based on multivariate logistic regression was created using decision curve analyses.

Reslut

The total number of patients included in this study was 6,388. The proportion of patients with pCR was 17.50% (n=1118), and the proportion of patients with primary tumor pCR was 20.84% (n = 1,331). The primary tumor was pCR in 16.00% (n=213) of the patients. Age, clinical T stage, clinical N stage, and histology were found to be significant independent clinical predictors of LNM using LASSO and multivariate logistic regression analysis. The nomogram was developed based on four clinical factors. The 5-year overall survival rate was 78.9 percent for those with ypN- and 66.3 percent for those with ypN+, respectively (P<0.001).

Conclusion

Patients over 60 years old, with clinical T1-2, clinical N0, and adenocarcinoma may be more likely to achieve ypN0. The watch-and-wait (WW) strategy may be considered. Patients who had ypN0 or pCR had a better prognosis.
Hereditary predisposition to malignant myeloid hemopathies: Caution in use of saliva and guideline based on our experience
Authors: Perani Alexandre , Bourthoumieu Sylvie , Rizzo David , Chauzeix Jasmine , Dauriat Benjamin , Turlure Pascal , Girault Stéphane , Veyrune Léa , Roubinet Maxime , Feuillard Jean , Yardin Catherine , Gachard Nathalie
Published: February 2023

Background

Predisposition to myeloid malignancies is a field at the border of hematology and genetics. Knowledge in this domain has so rapidly increased that WHO defined in 2016 the new “Myeloid Neoplasms with Germline Predisposition” category of tumors. High throughput sequencing is frequently performed in tumors either for diagnosis or prognosis, but this approach may identify potential germline variants that have to be confirmed on non-infiltrated tissues.

Method

In this study, we systematically compared NGS data from genetic analysis performed on all sample types (bone marrow, blood, saliva, skin fibroblasts and hair follicles) in 29 patients, and 44 of their relatives (blood and saliva).

Results

We showed that saliva was usable for relatives, but only for 24% (7/29) of our patients. Most of patients’ saliva were either “non-contributive” (14/29 i.e., 48% because clearly or probably infiltrated) or “inconclusive” (8/29 corresponding to 28%).

Conclusion

The recommendations for the use of saliva we present here focus on the importance of collecting saliva during remission when possible. Moreover, we propose hair follicles as an alternative to skin biopsy, that remains the gold standard especially in case of allogenic hematopoietic stem cells transplantation. Technological progresses have revolutionized the diagnosis of predisposition to solid or hematological malignancies, and it is very likely that new techniques will help to manage the familial predisposition in the future.
Predictive values of clinical data,molecular biomarkers, and echocardiographic measurements in preterm infants with bronchopulmonary dysplasia
Authors: Wang Huawei , Yan Dongya , Wu Zhixin , Geng Haifeng , Zhu Xueping , Zhu Xiaoli
Published: February 2023

Objective

We aimed to use molecular biomarkers and clinical data and echocardiograms that were collected during admission to predict bronchopulmonary dysplasia (BPD) in preterm infants with gestational age ≤32 weeks.

Methods

Eighty-two patients (40 with BPD, BPD group and 42 healthy as controls, non-BPD group) admitted to the Department of Neonatology of the Children's Hospital of Soochow University between October 1, 2018, and February 29, 2020, were enrolled in this study at the tertiary hospital. Basic clinical data on the perinatal period, echocardiographic measurements, and molecular biomarkers (N-terminal-pro-B-brain natriuretic peptide, NT-proBNP) were collected. We used multiple logistic regression analysis to establish an early predictive model for detecting BPD development in preterm infants of gestational age ≤32 weeks. We also used a receiver operating characteristic curve to assess the sensitivity and speciﬁcity of the model.

Results

No significant differences were found between the BPD and non-BPD groups in terms of sex, birth weight, gestational age, incidence of asphyxia, maternal age, gravidity, parity, mode of delivery, premature rupture of membranes >18 h, use of prenatal hormones, placental abruption, gestational diabetes mellitus, amniotic fluid contamination, prenatal infections, and maternal diseases. The use of caffeine, albumin, gamma globulin; ventilation; days of FiO2 ≥ 40%; oxygen inhalation time; red blood cell suspension infusion volume (ml/kg); and proportion of infants who received total enteral nutrition (120 kcal/kg.d) ≥24 d after birth were higher in the BPD group than in the non-BPD group. The levels of hemoglobin, hematocrit, and albumin in the BPD group were significantly lower than those in the non-BPD group. The total calorie intake was significantly lower in the BPD group on the 3rd, 7th, and 14th day after birth than in the non-BPD group (P < 0.05). The incidence rates of patent ductus arteriosus (PDA), pulmonary hypertension, and tricuspid regurgitation were significantly higher in the BPD group than in the non-BPD group (P < 0.05). The serum level of NT-proBNP 24 h after birth was significantly higher in the BPD group than in the non-BPD group (P < 0.05). Serum NT-proBNP levels were significantly higher in infants with severe BPD than in those with mild or moderate BPD (P < 0.05).

Conclusion

As there were various risk factors for BPD, a combining clinical data, molecular biomarkers, and echocardiogram measurements can be valuable in predicting the BPD. The tricuspid regurgitation flow rate (m/s), NT-proBNP (pg/ml), ventilator-associated pneumonia, days of FiO2 ≥ 40% (d), red blood cell suspension infusion volume (ml/kg), and proportion of infants who received total enteral nutrition (120 kcal/kg.d) ≥24 d after birth were the most practical factors considered for designing an appropriate model for predicting the risk of BPD.
Analysis of the impact of the SARS-CoV-2 infection on the pediatric population hospitalized during the pandemic in the Greater Paris University Hospitals
Authors: Semeraro Michaela , Pinson Pierre , Populaire Margaux , Dellagi Mourad , Oualha Mehdi , Beeker Nathanael , Chappuy Hélène
Published: February 2023

Background

The clinical characteristics, disease progression and outcome in children affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection appear significantly milder compared to older individuals. Nevertheless, the trends in hospitalization and clinical characteristics in the pediatric population seem to be different over time across the different epidemic waves.

Objective

Our aim was to understand the impact of the different COVID-19 variants in the pediatric population hospitalized in the Pediatric Departments of the Public Hospital in the Greater Paris area by the analysis performed with the Assistance Publique-Hopitaux de Paris (AP-HP) Health Data Warehouse.

Methods

This is a retrospective cohort study including 9,163 patients under 18 years of age, hospitalized from 1 March 2020 to 22 March 2022, in the Paris area, with confirmed infection by SARS-CoV-2. Three mutually exclusive groups with decreasing severity (Pediatric Inflammatory Multisystem Syndrome (PIMS), symptomatic infection, mild or asymptomatic infection) were defined and described regarding demography, medical history, complication of the SARS-CoV-2 infection, and treatment during admission. Temporal evolution was described by defining three successive waves (March–September 2020, October 2020–October 2021, and November 2021–March 2022) corresponding to the emergence of the successive variants.

Results

In the study period, 9,163 pediatric patients with SARS-CoV-2 infection were hospitalized in 21 AP-HP hospitals. The number of patients with SARS-CoV-2 infection increased over time for each wave of the pandemic (the mean number of patients per month during the first wave was 332, 322 during the 2nd, and 595 during the third wave). In the medical history, the most associated concomitant disease was chronic respiratory disease. Patients hospitalized during the third wave presented a higher incidence of pulmonary involvement (10.2% compared to 7% and 6.5% during the first and second waves, respectively). The highest incidence of PIMS was observed during the first and second waves (4.2% in the first and second waves compared to 2.3% in the 3rd wave).

Discussion

This analysis highlighted the high incidence of hospitalized children in the Greater Paris Area during the third wave of SARS-CoV-2 pandemic corresponding to the Omicron Covid-19 variant, which is probably an expression of a concomitant SARS-CoV-2, while a decreased incidence of PIMS complication was observed during the same period.
Design, synthesis and screening of indole acetic acid-based tri-azo moieties as antioxidants, anti-microbial and cytotoxic agents
Authors: Javaid Maryam , Haq Ihsan-Ul , Nadeem Humaira , Fatima Humaira , Khan Arif-Ullah , Irshad Nadeem
Published: February 2023

Multidrug resistance and infectious disease have enormous spread despite drug discovery and development advancements. 1, 2, 4 -triazoles have been extensively studied, playing an imperative role in many pathologic conditions. A series of Schiff base triazoles; derived from Indole -3- acetic acid with substituted Benzaldehydes (5a-5g) were designed, synthesized, and evaluated through various Spectroanalytical techniques. SwissADME was used to assess physicochemical properties and pharmacokinetic drug-likeliness behavior. (5a-5g) were evaluated for their varied biological potential through antioxidant, antimicrobial, enzyme inhibition, and cytotoxic evaluation. Schiff bases express drug-like nature as they follow Lipinski’s rule of five. 5b showed good antioxidant potential in total antioxidant capacity (TAC) and total reducing power (TRP) assays and was most active in the library in % free radical scavenging assay (%FRSA), showing 32% inhibition at 50 μg/mL concentration. Compounds showed antibacterial activity against various tested strains. 5e and 5f showed a minimum inhibitory concentration (MIC) value of 3.12 μg/mL for P.aeruginosa and K.pneumoniae, respectively. In the antifungal assay, only 5e inhibited one strain with a zone of inhibition >6 mm. These synthetic molecules possess good cytotoxic potential in the Brine Shrimp Lethality screening; 5c, 5d, and 5f exhibited LC50 =5.7 μg/mL. In the protein kinase inhibition assay, 5a, 5b, and 5g demonstrated inhibitory potential, showcasing the zone of inhibition as 7.5–10.5 mm for the bald one and 6–7.5 for the clear zone. These findings suggest that the compounds have antibacterial and cytotoxic potential, and there is a chance for further research and development in this area.
Glycyrrhizin for treatment of CRS caused by CAR T-cell therapy: A pharmacological perspective
Authors: Qi Xingxing , Li Juan , Luo Pan
Published: February 2023

Chimeric antigen receptor T (CAR T)-cell therapy promises to revolutionize the management of hematologic malignancies and possibly other tumors. However, the main side effect of cytokine release syndrome (CRS) is a great challenge for its clinical application. Currently, treatment of CRS caused by CAR T-cell therapy is limited to tocilizumab (TCZ) and corticosteroids in clinical guidelines. However, the theoretical risks of these two agents may curb clinicians’ enthusiasm for their application, and the optimal treatment is still debated. CAR T-cell therapy induced-CRS treatment is a current research focus. Glycyrrhizin, which has diverse pharmacological effects, good tolerance, and affordability, is an ideal therapeutic alternative for CRS. It can also overcome the shortcoming of TCZ and corticosteroids. In this brief article, we discuss the therapeutic potential of glycyrrhizin for treating CRS caused by CAR T-cell therapy from the perspective of its pharmacological action.
Cultivating a culture of inclusivity in heliophysics
Authors: Halford Alexa J. , Burrell Angeline G. , Liemohn Michael W. , Jones McArthur , Maute Astrid , Pulkkinen Tuija I. , Bard Christopher M. , McGranaghan Ryan M. , Wilson Lynn B. , Allen Robert C. , Dong Chuanfei , Vines Sarah K. , Wang Liang , Turner Niescja , Garcia-Sage Katherine , Mandt Kathleen , Klenzing Jeff
Published: February 2023

A large number of heliophysicists from across career levels, institution types, and job titles came together to support a poster at Heliophysics 2050 and the position papers for the 2024 Heliophysics decadal survey titled “Cultivating a Culture of Inclusivity in Heliophysics,” “The Importance of Policies: It’s not just a pipeline problem,” and “Mentorship within Heliophysics.” While writing these position papers, the number of people who privately shared disturbing stories and experiences of bullying and harassment was shocking. The number of people who privately expressed how burned out they were was staggering. The number of people who privately spoke about how they considered leaving the field for their and their family’s health was astounding. And for as much good there is in our community, it is still a toxic environment for many. If we fail to do something now, our field will continue to suffer. While acknowledging the ongoing growth that we as individuals must work toward, we call on our colleagues to join us in working on organizational, group, and personal levels toward a truly inclusive culture, for the wellbeing of our colleagues and the success of our field. This work includes policies, processes, and commitments to promote: accountability for bad actors; financial security through removing the constant anxiety about funding; prioritization of mental health and community through removing constant deadlines and constant last-minute requests; a collaborative culture rather than a hyper-competitive one; and a community where people can thrive as whole persons and do not have to give up a healthy or well-rounded life to succeed.
Transcriptomics explores the potential of flavonoid in non-medicinal parts of Saposhnikovia divaricata (Turcz.) Schischk
Authors: Chen Yuqiu , Zhang Tao , Chen Changbao , Xu Zhefeng , Liu Chunshuo
Published: February 2023

Introduction

Saposhnikovia divaricata is a traditional Chinese medicine in China, which is widely used in clinic. The root of S. divaricata is often used as medicine, but little research has been done on its other tissues.

Methods

In this study, the contents of root and leaf of S. divaricata were determined by HPLC, the differentially expressed genes were screened by transcriptome sequencing at molecular level, and then verified by network pharmacology.

Results

The results showed that the content of 4’-O-β-D-glucosyl-5-O-methylvisamminol in the leaves was significantly higher than that in the roots, which was about 3 times higher than that in the roots. In addition, 10 differentially expressed key enzyme genes were screened in plant hormone signal transduction, phenylpropanoid and flavonoid biosynthetic pathways. C4H and CYP98A were up-regulated in root, while F3H was down-regulated in root. They can be used as important candidate genes for the mechanism of quality difference of S. divaricata. Finally, network pharmacological validation showed that 5-O-methylvesamitol plays an important role in the treatment of ulcerative colitis.

Discussion

These findings not only provide insight into flavonoid biosynthesis in S. divaricata associated molecular regulation, but also provide a theoretical basis for the development and utilization of S. divaricata.
Robustness of calibration model for prediction of lignin content in different batches of snow pears based on NIR spectroscopy
Authors: Wu Xin , Li Guanglin , Fu Xinglan , Wu Weixin
Published: February 2023

Snow pear is very popular in southwest China thanks to its fruit texture and potential medicinal value. Lignin content (LC) plays a direct and negative role (higher concentration and larger size of stone cells lead to thicker pulp and deterioration of the taste) in determining the fruit texture of snow pears as well as consumer purchasing decisions of fresh pears. In this study, we assessed the robustness of a calibration model for predicting LC in different batches of snow pears using a portable near-infrared (NIR) spectrometer, with the range of 1033–2300 nm. The average NIR spectra at nine different measurement positions of snow pear samples purchased at four different periods (batch A, B, C and D) were collected. We developed a standard normal variate transformation (SNV)-genetic algorithm (GA) -the partial least square regression (PLSR) model (master model A) - to predict LC in batch A of snow pear samples based on 80 selected effective wavelengths, with a higher correlation coefficient of prediction set (Rp) of 0.854 and a lower root mean square error of prediction set (RMSEP) of 0.624, which we used as the prediction model to detect LC in three other batches of snow pear samples. The performance of detecting the LC of batch B, C, and D samples by the master model A directly was poor, with lower Rp and higher RMSEP. The independent semi-supervision free parameter model enhancement (SS-FPME) method and the sequential SS-FPME method were used and compared to update master model A to predict the LC of snow pears. For the batch B samples, the predictive ability of the updated model (Ind-model AB) was improved, with an Rp of 0.837 and an RMSEP of 0.614. For the batch C samples, the performance of the Seq-model ABC was improved greatly, with an Rp of 0.952 and an RMSEP of 0.383. For the batch D samples, the performance of the Seq-model ABCD was also improved, with an Rp of 0.831 and an RMSEP of 0.309. Therefore, the updated model based on supervision and learning of new batch samples by the sequential SS-FPME method could improve the robustness and migration ability of the model used to detect the LC of snow pears and provide technical support for the development and practical application of portable detection device.
Comparative transcriptomic and metabolomic analyses reveal differences in flavonoid biosynthesis between PCNA and PCA persimmon fruit
Authors: Wang Yiru , Suo Yujing , Han Weijuan , Li Huawei , Wang Zhenxu , Diao Songfeng , Sun Peng , Fu Jianmin
Published: February 2023

The fruit of the persimmon (Diospyros kaki.) has high economic and nutritional value and is rich in flavonoids. Flavonoids are essential secondary metabolisms in plants. The association between persimmon astringency and changes in the proanthocyanidins (a flavonoid subclass) content is well-known. However, information on the relationships between different astringency types and other flavonoid subclasses and biosynthetic genes is more limited. In this study, an initial correlation analysis between total flavonoids and fruit astringency type, and KEGG analysis of metabolites showed that flavonoid-related pathways were linked to differences between mature pollination-constant non-astringent (PCNA) varieties (‘Jiro’ and ‘Yohou’) and pollination-constant astringent (PCA) fruit varieties (‘Zhongshi5’ and ‘Huojing’). Based on these findings, variations in the expression of genes and metabolites associated with flavonoid biosynthesis were investigated between typical PCNA (‘Jiro’) and PCA (‘Huojing’) persimmons during fruit development. The flavonoid concentration in ‘Huojing’ fruit was significantly higher than that of ‘Jiro’ fruit, especially, in levels of proanthocyanin precursor epicatechin and anthocyanin cyanidin derivatives. Combined WGCNA and KEGG analyses showed that genes such as PAL, C4H, CHI, CHS, F3H, F3’5’H, FLS, DFR, ANR, ANS, and UF3GT in the phenylpropanoid and flavonoid biosynthesis pathways may be significant factors impacting the proanthocyanin precursor and anthocyanin contents. Moreover, interactions between the R2R3MYB (evm.TU.contig7272.598) and WD40 (evm.TU.contig3208.5) transcription factors were found to be associated with the above structural genes. These findings provide essential information on flavonoid biosynthesis and its regulation in the persimmon and lay a foundation for further investigation into how astringency types affect flavor components in PCNA and PCA persimmons.