Figure 7 from Functional RNAi Screening Identifies G2/M and Kinetochore Components as Modulators of TNFα/NF-κB Prosurvival Signaling in Head and Neck Squamous Cell Carcinoma
Authors: Morgan Ethan L. , Saleh Anthony D. , Cornelius Shaleeka , Carlson Sophie G. , Toni Tiffany , Cheng Hui , Jeon Jun , Viswanathan Ramya , Yang Xinping , Silvin Christopher , Clavijo Paul E. , Sowers Anastasia L. , Mitchell James B. , Ormanoglu Pinar , Lal Nag Madhu , Martin Scott E. , Chen Zhong , Van Waes Carter 
Published: November 2024

<p>High TTK expression correlates with survival in HNSCC. <b>A,</b> Box plot analysis of <i>TTK</i> mRNA expression in normal (<i>n</i> = 44) and HNSCC (<i>n</i> = 516) tissue, in normal (<i>n</i> = 44), HPV<sup>−</sup> HNSCC (<i>n</i> = 434) and HPV <sup>+</sup> HNSCC (<i>n</i> = 80) tissue and in normal (<i>n</i> = 44), WT TP53 HNSCCs (<i>n</i> = 167), and mtTP53 HNSCCs (<i>n</i> = 347) tissue from TCGA HNSCC database. <b>B,</b> Correlation of gene copy number with mRNA expression in HNSCC based on HPV status. CNVs were presented on the <i>X</i> axis, as one-copy loss (−1), diploid (0), one-copy gain (0.58), and amplification (larger than 1). mRNA expression calculated by RSEM is presented in the <i>y</i> axis as the log<sub>2</sub> scale. Pearson correlation tests were conducted for 508 HNSCC tumor specimens, including 428 HPV<sup>−</sup> (blue circles) and 80 HPV(+) samples (red triangles). <i>P</i> values give the significance of the Pearson correlation between mRNA expression and CNV. <i>R</i> values represent Pearson correlation coefficients. <b>C,</b> PFS and OS analysis of TCGA HNSCC data based on <i>TTK</i> expression, separated by HPV status. Survival data were plotted using the Kaplan–Meier survival curve. Red indicates high expression, and cyan indicates low expression. <i>P</i> values were determined using the log-rank test. Plots were truncated at 10 years for HPV<sup>−</sup> patients and 5 years for HPV<sup>+</sup> patients, but the analyses were conducted using all data. <b>D,</b> Scatter dot plot of <i>TTK</i> mRNA expression in normal and HNSCC tissue from the GEO database entries GSE6791 (normal <i>n</i> = 14, HNSCC <i>n</i> = 42) and GSE25099 (normal <i>n</i> = 22, HNSCC <i>n</i> = 57). <b>E,</b> Box plot analysis of TTK protein expression in normal (<i>n</i> = 58) and HNSCC (<i>n</i> = 101, all HPV<sup>−</sup>) tissue from the CPTAC dataset. <b>F,</b> PFS and OS analysis of CPTAC HNSCC data based on TTK protein expression. Survival data were plotted using the Kaplan–Meier survival curve from 85 HPV<sup>−</sup> HNSCCs cases that had survival data. Red indicates high expression, and cyan indicates low expression. <i>P</i> values were determined using the log-rank test. <b>G,</b> PFS analysis of TCGA HNSCC data based on <i>TTK</i> expression and if patients were treated with radiotherapy or not, separated by HPV status. Survival data were plotted using the Kaplan–Meier survival curve. Blue indicates high expression with no radiotherapy, gold indicates high expression with radiotherapy, gray indicates low expression with no radiotherapy, and red indicates low expression with radiotherapy. From the TCGA data, 59 HPV<sup>−</sup> and 8 HPV<sup>+</sup> cases had no radiotherapy record, so they were excluded from the analysis. <i>P</i> values were determined using the log-rank test. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001 (Student <i>t</i> test). NS, not significant; CNV, copy number variations; GEO, Gene Expression Omnibus; WT, wildtype.</p>