bluuebunny/crossref_metadata_2025 huggingface dataset:
Paired Sensitivity Analysis of Four SARS-CoV-2 Serological Immunoassays in a Longitudinal Cohort of Convalescent Hospital Staff
Authors: Sim Marcus , Cockcroft Christopher , Darby Denise , Ellis Clare , Heaps Adrian , Scargill Jonathan , Garcez Tomaz
Published: January 2024

BACKGROUND: SARS-CoV-2 serological testing has seen extensive academic
and clinical use from investigating correlates of immunity to
seroprevalence, convalescent plasma and vaccine trials. Interpretation
of these studies will depend on robust validation of the longitudinal
sensitivities of serological assays. OBJECTIVE: To conduct longitudinal
sensitivity analysis on four different SAR-CoV-2 antibody assays in a
convalescent cohort with predominantly mild Coronavirus Disease 2019
(COVID-19). STUDY DESIGN: Hospital staff (n=94) returning to work
following polymerase chain reaction (PCR) confirmed COVID-19 were
offered antibody testing to assist with laboratory verification. Initial
specimens were collected at median 29 days post-symptom onset and run on
the Roche, Abbott, Siemens and DiaSorin platforms. Re-sampling occurred
at median 142 days from a subset of the initial cohort (n=62) that had
volunteered to provide further sera to assist in longitudinal
sensitivity analysis. Samples that were not run across all four
platforms were excluded from analysis. RESULTS: Comparative sensitivity
analysis was conducted on 89/94 of the initial specimens and 55/62 of
the repeat specimens. Sensitivity at initial sampling ranged from
78-87% across platforms. At re-sampling, sensitivities were: 100%
(Roche), 45% (Abbott), 100% (Siemens), and 80% (DiaSorin). Paired
analysis using the longitudinal cohort (n=55) demonstrated stable or
increasing median assay values on three platforms, with a clear
reduction seen only on the Abbott platform (4.78 to 1.34) with
corresponding sensitivity drop-off. CONCLUSION: The Abbott assay
demonstrated sensitivity drop-off and decrease in median assay signal
below detection threshold at 4-5 months. This has implications on the
interpretation and design of future studies
Multiple replacements for recurrent stuck mechanical valve in a patient with Fontan circulationtech
Authors: Agematsu Kota , Nagashima Mitsugi , Nishimura Yoshiharu
Published: January 2024

Significant atrioventricular valve insufficiency is associated with
increased mortality and morbidity in patients with a single-ventricle
because of the congestion associated with single-ventricle circulation.
Treatment of atrioventricular valve insufficiency is mandatory for
completing Fontan circulation. Although valve repair is a favorable
procedure for the lesion because it preserves the valve leaflet and
ventricular motion, replacement of the atrioventricular valve is the
last option of treatment. The choice of a mechanical valve is often made
for atrioventricular valve replacement in children. However, because
children’s small body size, mechanical valve implantation can cause
early and late complications, including thromboembolic or hemorrhagic
events, the artificial valve leaflet becoming stuck, and ventricular
dysfunction. Because of these complications, valve replacement can be
performed several times, and repeat surgical intervention can induce
technical difficulties of artificial valve implantation as well. We
present a patient who received multiple mechanical valve replacements
due to repeat valve leaflet stuck after first palliation of pulmonary
artery banding followed by Glenn anastomosis and Fontan completion. To
overcome surgical difficulty, some technical modification was required
during the repeat valve replacement.
Postoperative coronary artery spasm after mitral valve replacement and Cox-Maze IV procedure: A case report
Authors: Lang Qianlei , Li Chunyan , Qin Chaoyi , Meng Wei
Published: January 2024

Background: Little case was reported about coronary artery spasm after a
mitral valve replacement and concomitant Cox-Maze IV procedure. We
reported a case of an adult male who develop right coronary artery (RCA)
spasm after a mitral valve replacement with tricuspid valve repair and
Cox-Maze IV procedure. Case Report: A 66-year-old male, complaining
progressive exertional shortness of breath, was diagnosed as severe
mitral stenosis, moderate tricuspid regurgitation, complete right bundle
branch block and persistent atrial fibrillation (AF) in our clinic. The
patient underwent elective mitral valve replacement, tricuspid valve
repair and Cox-Maze IV procedure. 4 h after surgery, a 12-lead
electrocardiogram (ECG) showed progressive elevation of ST-segment in
the avF and III leads and Troponin-T was over 7000 pg/mL. After 1 h,
Troponin-T increased to over 10000 pg/mL and ECG still showed persisted
ST-segment elevation in inferior leads. Emergent angiography was
performed and intra-coronary administration of nitroglycerin completely
relieved the spasm. KEYWORDS mitral valve replacement, Cox-Maze IV,
coronary artery spasm
Comparative Analysis of Four Commercial RT-PCR Diagnostic Assay for Detection of Covid-19
Authors: Hasanzadeh Sepideh , Jangi Mahbubeh , Mehri Ali , Khaniki4 Saeedeh Hajebi , Azari Behnam , Jamehdar Saeid Amel
Published: January 2024

SARS-CoV-2 real-time reverse-transcription PCR (rRT-PCR) is currently
the only available yet effective testing system to fight COVID-19
epidemics as far as there’s not any treatment and vaccine. Moreover,
many SARS-CoV-2 rRT-PCR kits are approved by the
emergency-use-authorization (EUA) altogether over the world. In this
article we’ve provided a comparison of important performance features of
four commercial RT-PCR assays. A total of consecutive nasopharyngeal
(NPS) samples and oropharyngeal (OP) swabs were collected from 50
COVID-19 patients for sensitivity and specificity analysis. Specificity
of these assays were examined by using extractions of RNA from common
human coronavirus cultures. All RT-PCR kits including in this study
exhibited acceptable specificity over 90%, except for the Sansure and
PowerCheck (88%). Pishtaz teb assays demonstrated a PPA of 95.24%
(40/42), while the DaAn Gene, Sansure, Power check of SARS-CoV-2 panel
showed the PPA of 85.7% (36/42), 66.66% (28/42), and 64.3% (27/42),
respectively. An NPA of 100% (8/8) was observed for four molecular
assays. This study gives a technical baseline of four distinct
commercial PCR assays for detection of SARS-CoV-2, that can be practical
and useful for laboratories interested in buying any of them for more
clinical validation.
Total Flavonoids of Astragalus activate T cell anti-tumor immunity by reducing myeloidderived suppressor cells in 4T1 mammary tumor mice
Authors: Wang Jing , Li Ke , Liu Yue , Qin Xuemei , Wu Changxin
Published: January 2024

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population
of immature myeloid cells that increase in tumor-bearing hosts and
suppress anti-tumor immunity to promote tumor growth and development.
Thus, reducing the number or inhibiting the function of MDSCs may
enhance anti-tumor immunity. Total flavonoids of Astragalus (TFA) are
the main active components of Astragalus. The immunoregulatory effects
of TFA have recently been extensively studied. However, the effect of
TFA on MDSCs still remaines unknown. In the present study, we found that
TFA treatment significantly alleviated the inhibitory effect of MDSCs on
T cell proliferation in 4T1 mammary tumor mice. TFA decreased MDSCs
accumulation in the bone marrow(BM), circulating blood, spleen, and
tumor bed, whereas increased the percentage and activation of T cells in
4T1 mammary tumor mouse model. In addition, TFA treatment significantly
reduced the number of MDSCs in BM cells induced by GM-CSF or the tumor
burden in vitro. Mechanistically, the reduction of MDSCs was partially
caused by increasing intracellular ROS level, which increased the
apoptosis of Gr-1+ cells. In addition,TFA reduced the expression of iNOS
and Arg-1 in both protein and transcription level. In conclusion,TFA
could activate systemic T cell immunity by inducing apoptosis and
inhibiting the function of MDSCs, suggesting that TFA has potential
clinical benefits as it selectively attenuates MDSC-induced
immunosuppression.
Molecular dynamics study of conformation transition from helix to sheet of Aβ42 peptides
Authors: Zhou Min , Wen Huilin , Lei Huimin , Zhang Tao
Published: January 2024

Aβ42 peptides can form helix and sheet structure under different
conditions. The conformational conversion is closely associated with Aβ
peptides aggregation and their neurotoxicity. But the transition from
helix to sheet is not be clearly understood. In this study we performed
microsecond timescale MD simulations of Aβ42 peptides to investigate the
conformation transition from α-helix to β-sheet. Markov state model
(MSM) was built to facilitate identification of crucial intermediate
states and possible transition pathway. Based on the analysis, we found
that the region Y10-A21 in the middle of Aβ42 peptide plays an initial
role in this transition. MSM model revealed that the collapse of helical
structure in this region might trigger the formation of sheet structure.
Moreover, we further simulated the aggregation of Aβ42 peptides with
different conformations. We found that the Aβ42 peptides forming sheet
structure have higher aggregation potential compared with peptides with
helix structure. These results demonstrate that we can prevent the
aggregation of Aβ42 peptides by stabilizing the helix structure in the
region of Y10-A21. In addition, this study provides new insight into
better understanding the conformational transition and aggregation of
Aβ42 peptides.
Acceptability of a personalized lifestyle program in women with levothyroxine-treated primary hypothyrodism with residual symptoms
Authors: Lisberg Lisa , Törsleff-Berglund Sanna , Palmlöf Lisa , Sandström Helene , Tillberg Anna , Noland Diana , Andreasson Anna , Brismar Kerstin
Published: January 2024

Rationale, aims and objectives: The aim of the intervention study was to
test the feasibility and acceptability of a time consuming and demanding
lifestyle-based treatment program in women with levothyroxine-treated
primary hypothyrodism with residual symptoms. In addition, the influence
of the intervention on participants’ health-related quality of life was
assessed. Methods: 14 participants were recruited through an online
questionnaire of symptom burden and health related quality of life in
patients with levothyroxine-treated primary hypothyroidism. The
intervention consisted of three parts: 1) diet including dietary
supplements; 2) stress and sleep management; 3) physical exercise.
Participants were interviewed at the end of intervention, and 6 and 12
months after intervention. Follow-up questionnaire assessements of
health related quality of life (WHODAS 2.0) were made at 6, 12, 18 and
24 months after start of intervention. Results: A very low drop-out rate
– 1 in 14 – and a high attendance rate indicate that the intervention
program was feasible. Compliance was high, particularly regarding
dietary changes and dietary supplement intake. Health-related quality of
life improved; the WHODAS score was decreased with 8.5 points after 3
months intervention (p=0.004), 8.8 points at end of treatment (p=0.008)
and 7.3 points at 12 months after the treatment ended (p=0.040, mixed
effect regression analysis), while no significant change was seen 6
months after treatment (4.1 points, p=0.17). Conclusion: The
intervention program was feasible and well accepted by the participants,
who reported improvements in health-related quality of life after
treatment. The efficacy of such program should be evaluated in future
randomized controlled studies.
QM/MM-based Rational Design of an Orthogonal X∙∙∙O∙∙∙H Interaction System at the Complex Interface of EGFR Kinase Domain with RALT Third Hotspot Peptide
Authors: Gu Hualong , Liu Lijun
Published: January 2024

Human epidermal growth factor receptor (EGFR) has been established as a
primary druggable target for the treatment of patients with diverse
cancers. The receptor-associated late transducer (RALT) protein is a
negative regulator of EGFR signaling by using its Seg1 segment to bind
at the allosteric dimerization interface of EGFR kinase domain to
disrupt the dimerization and then inactivate the kinase. Previous
studies found that the Seg1 adopts three separated hotspots to interact
with the EGFR dimerization interface, in which the third hotspot (TH) is
located at the core region of the interface but its derived TH peptide
(356PKYVS360) and Tyr358Phe mutant (356PKPVS360) cannot bind effectively
to the interface in an independent manner (KD > 1000 μM).
In this study, we demonstrate that the TH peptide can be converted from
nonbinder to a moderate binder of EGFR by just adding an orthogonal
nonbonded interaction system (X∙∙∙O∙∙∙H) between a halogen bond and a
hydrogen bond involving peptide Phe358 residue and EGFR Val948/Trp951
residues. Both the hybrid quantum mechanics/molecular mechanics (QM/MM)
and electron-correlation quantum chemistry calculations are utilized to
rigorously characterize and rationally design the X∙∙∙O∙∙∙H system,
which is then optimized with different halogen atoms and at different
substituting positions. It is revealed that there is a synergistic
effect between the halogen bond (X∙∙∙O) and hydrogen bond (H∙∙∙O) of the
orthogonal interaction system; formation of the halogen bond can enhance
the interaction strength of the hydrogen bond. In silico energetic
analysis and in vitro fluorescence assay reach a consistence that
Br-substitution at the m-position of peptide Phe358 phenyl moiety is the
best choice that can render strong interaction for the X∙∙∙O∙∙∙H system
(ΔEX•••O = -3.48 kcal/mol for X∙∙∙O and ΔEH•••O = -2.57 kcal/mol for
H∙∙∙O), which also makes the peptide ‘bindable’ to EGFR kinase domain
(KD = 247 μM), while F/Cl/I-substitution at the same position can only
improve the peptide affinity moderately or modestly (KD = 835/582/690
μM). In contrast, the Br-substitution at the o- and p-positions of
peptide Phe358 phenyl moiety cannot define effective X∙∙∙O∙∙∙H
interaction and thus does not confer additional affinity to the
EGFR–peptide complex (KD > 1000 μM).
Conserved Restricted Solid-on-Solid Model
Authors: Savu Anamaria
Published: January 2024

We study a model for the movement of surfaces, namely the conserved,
restricted solid-on-solid model. The surface configurations are
restricted such that the difference between the heights at adjacent
sites is no more than one. In addition, the total number of particles is
preserved by the dynamics of the model. Mean-field approximations are
used to approximate the one-site probability function of the model.
Targeting COVID-19 Based on Pathogenesis
Authors: Chong Zhao Zhong , Zhang Hongjun
Published: January 2024

The new coronavirus disease 2019 (COVID-19) caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) are causing health
crisis of the world. To control the pandemic SARS-CoV-2 infection,
world-wide vaccination are under way. However, no specific therapeutic
drugs are available in the treatment of COVID-19 yet. To find the
effectively therapeutic targets for COVID-19, efforts should be focused
on the pathogenesis of COVID-19. SARS-CoV-2 induced COVID-19 involves
the processes of viral entry into the host cells, viral replication in
the host cells, and induction of cytokine storm and cellular damage.
Therefore, the potential targets may include the structural proteins of
the virus that play roles in the pathogenesis, viral replication
associated enzymes, inflammatory cytokines, and signaling proteins that
mediate the induction of cytokine storm. Further exploring this strategy
may benefit us in finding novel therapeutic targets and effective
treatment of COVID-19.
Qualitative and quantitative analysis of secondary metabolites in morphological parts of Paulownia Clon in Vitro 112®
Authors: Stochmal Anna , Moniuszko-SZajwaj Barbara , Zuchowski Jerzy , Pecio Łukasz , Szumacher-Strabel Malgorzata , Grabarczyk Łukasz , Olas Beata , Cieślak Adam
Published: January 2024

It is not easy to find data in the scientific literature on the
quantitative content of individual phytochemicals. It is possible to
find groups of compounds and even individual compounds rather easily,
but it is not known what their concentration is in cultivated or wild
plants. Therefore, the subject of this study was to determine the
content of individual compounds in the new Paulownia species - Oxytree,
developed in a biotechnology laboratory in 2008 at La Mancha University
in Spain. Six secondary metabolites were isolated, and their chemical
structure was confirmed by spectral methods. An analytical method was
developed, which was then used to determine the content of individual
compounds in leaves, twigs, flowers and fruits of Paulownia Clon in
Vitro 112®. No flavonoids were found in twigs and fruits of Oxytree,
while the highest phenylethanoid glycosides were found in twigs.
A case of multiple arrhythmias caused by PCI(Percutaneous coronary intervention)
Authors: Shao Xin , Wang Haixiong , Han Xuebin
Published: January 2024

PCI(Percutaneous coronary intervention) may lead to the occlusion of the
artery of sinoatrial node, and it is rare that the occlusion of the
sinoatrial node artery simultaneously causes sick sinus syndrome and
atrial tachycardia. A clinical case has been encountered and the report
is as follows.
Reinfection with SARS-CoV-2: A dilemma in clinical practice
Authors: Barary Mohammad , Afshar Zeinab Mohseni , Sayad Babak , Janbakhsh Alireza , Ebrahimpour Soheil , Babazadeh Arefeh
Published: January 2024

There is a growing concern about the reinfection of recovered patients
with novel coronavirus disease 2019 (COVID-19). Various researches have
shown that the Reinfection of COVID-19 is probable, but its risk and
probability vary considerably. Positive reverse transcription-polymerase
chain reaction (RT-PCR) retest results are not necessarily defined as
reinfections, as several factors may be responsible for this
re-positivity in the laboratory. Therefore, the utilization of tests
with higher sensitivity and further clinical examinations can help
distinguish true reinfection. This review is focused on the latest
findings of COVID-19 reinfection in recovering patients.
An Enhanced Dictionary based approach for identify the biomedical entities from PubMed Articles
Authors: GOVINDARAJ SUGANYA , R Porkodi
Published: January 2024

Data mining is characterized as a process of transforming data
information into a human-comprehensible code format such as rules,
formula, algorithm, and so on. Bioinformatics is developed to solve a
biological problem by using data mining technique. Identifying
biomedical domain entities is a difficult task and the enhanced model is
used to classify the entities from biomedical literature full text
articles in PubMed database. The enhanced model 3 stages namely
pre-processing, identification of the entities using dictionary-based
approach and verification, validation with benchmarking databases. The
model, Disgenet and Pubtator are considered as the dictionary which is a
benchmarking database. This approach defines entities using the
en_ner_bionlp13cg_md model from spacy package. Experimental purposes,
99 full text articles related to Alzheimer’s disease are considered
which are downloaded from NCBI. Finally, demonstrated that our enhanced
model for dictionary based approach outperforms in aspects of accuracy,
precision and retrieval value. The model achieved 82% accuracy overall.
Compared to state-of -art method, model obtained the better accuracy.
These results suggested that the enhanced model is obtained high
performance for extracting biomedical entities from PubMed articles. The
improvement mostly due to the dictionary because Disgenet and Pubtator
are considered the dictionary.
Quality of life improvements after bariatric surgery in reproductive age women with and without PCOS: a cohort study
Authors: Lacey Hester , Attersley-Smith Stephanie , Hart Kathryn , Whyte Martin , Shawe Jill , Stone Sophia
Published: January 2024

Objective: To understand the impacts of bariatric surgery on quality of
life (QOL) in reproductive age women both with and without PCOS. Design:
A cohort study assessing QOL prior to and a 3,6 and 12 month follow up
appointments post bariatric surgery. Setting: Women completed
questionnaires at clinic appointments or surveys posted to them for
completion. Population or Sample: Sample taken from the RHAMS study
(Reproductive Health after Metabolic Surgery). Methods: The BOSS42
Bariatric & Obesity-Specific Survey was used to assess QOL. Main
Outcome Measures: QOL improvements were assessed over time and
differences between the two groups compared. Secondary outcomes included
assessment of hirsutism, irregular or absent periods, and Sex Hormone
Binding Globulin levels pre- and post-surgery. Results: Bariatric
surgery was shown to have significant QOL benefits for both groups.
Resolution rates of irregular or absent periods was significant across
both groups. Resolution of hirsutism was significant in women with PCOS
after weight loss. Weight was significantly negatively correlated with
QOL. Conclusions: Bariatric surgery results in significant physical and
psychological health benefits for all women with obesity, and for women
with PCOS, may alleviate a significant amount of disease related burden
related to hyperandrogenism, sub-fertility, and metabolic disturbance.
These results indicate the significance and breadth of benefits
bariatric surgery can have for women both with and without PCOS. Funding
This research did not receive any specific grant from funding agencies
in public, commercial, or not-for-profit sectors. Keywords: ‘bariatric
surgery’ ‘PCOS’ ‘quality of life’ MESH* terms - Fertility*,
Reproduction*, Women’s Health
The influence of general anesthesia on catheter-related parameters during pulmonary vein isolation
Authors: Gao Jia , Zhang Bing , Zhang Nan , Meng Sun , Tian Shouyuan , Wang Rui
Published: January 2024

Abstract Aims: Pulmonary vein isolation (PVI) is the main treatment of
atrial fibrillation (AF), which can be performed under general
anesthesia or conscious analgesia. The use of general anesthesia may
improve contact force (CF) and mapping system accuracy compared to
conscious sedation, but its influence on catheter swing and ablation
index (AI) during PVI has not previously been investigated. Methods: The
study population included 20 patients with Af who first underwent PVI
procedure:10 patients adopted general anesthesia (GA) and 10 patients
adopted conscious sedation (CS). We retrospectively analyzed the
difference of catheter swing, CF, AI and distance between ablation
points during PVI in two groups. Results: The swing of catheter was
significantly higher in the CS group than in the GA group. On the
contrary, CF increased in GA group. The AI and the distance between
ablation points has no difference between the groups. In addition, GA
was associated with lower pulmonary vein reconnection, total ablation
points and supplemental ablation points. Conclusion: GA reduced catheter
swing and improves CF during PVI compared with CS.
URETER ADHESION TO FIBROIDS
Authors: Tjokroprawiro Brahmana Askandar
Published: January 2024

This study describes a patient with fibroid who underwent a hysterectomy
and it was complicated by the adhesion of one the patient’s ureters to
the fibroid. The adhesion of may substantially distort the normal
appearance of the ureter. Careful attention to the ureter is necessary
for large retroperitoneal fibroid surgery
Reliability Analysis of a Mechanical System with 3 Out of 5 Subsystems Using Excel Solver
Authors: B Yamuna , Gupta Radha , Ramesh KoKila , N Nethravathi
Published: January 2024

Optimization of a redundancy allocation problem designed specifically
for systems with k-out-of-n subsystems has been the motive of this work.
The main motive is to select the components and redundancy levels to
maximize system reliability given system-level constraints. With cost
and weight as constraints as 3 out of 5 subsystems has been optimized in
the present study for maximizing the reliability of a mechanical system.
Component reliabilities in every stage are treated as unknowns in this
model. Evolutionary and Generalised Reduced Gradient method (GRG
nonlinear Solver) in Excel has been applied here to determine the
component reliabilities, stage reliabilities to maximize system
reliability. A comparative study has been done using the methodology in
the present paper with the methods already used by the other authors for
the similar systems.
Solutions of coupled KdV and BBM equations in weighted Sobolev spaces
Authors: GUTIERREZ HERNAN OSCAR , GUTIERREZ MILTON
Published: January 2024

In this article, we analyze the existence of the solutions of coupled
KdV and BBM equations in a weighted Sobolev space ( wSs-BBM) with
initial data u_t- u_xxt-av_xxt+a_1 u_x+a_2 v^p v_x+u^p
u_x+a_3 〖(u^p v)〗_x=0 v_t- v_xxt-au_xxt+a_1 v_x+a_2 u^p
u_x+v^p v_x+a_3 〖(uv^p)〗_x=0 We use the theory of the
semigroups of linear operators and Lax-Milgram lemma for the existence
for the Korteweg de Vries equations (wSs-KdV).
A Systematic and Comparative Analysis of Four Major Classes of DFT Functionals to Compute Linear and Nonlinear Optical Properties of Benchmark Molecules
Authors: Waheed Alina , Muhammad Shabbir , Gilani Mazhar , Adnan Muhammad , Aloui Zouhaier
Published: January 2024

The present study spotlights the fundamental insights about the
systematic and comparative analysis of four famous hybrid classes of
density functional theory (DFT) methods and their efficacy to calculate
the linear and nonlinear optical (NLO) polarizabilities. For this study,
urea and para-nitroaniline (p-NA) molecular geometries are used as
prototype to calculate their linear and NLO properties. For comparative
purpose, these molecules are often used as reference organic molecules
for determination of NLO response properties and there is a dire need of
such benchmark database to be utilized by the researchers. We report
systematically a range of functionals including hybrid (B3LYP, PBE1PBE,
BHandHLYP), meta-hybrid (M06, M06-2X, M06-HF, M06-L), long-range
corrected (CAM-B3LYP, LC-BLYP, LC-B97D, LC-B97D3) and functional with
dispersion correction (ωB97, ωB97X, ωB97XD, HSEH1PBE). These groups are
evaluated and their efficiency to calculate linear and NLO properties is
graphically compared with each other. Overall, there is less deviations
among different functionals for calculating dipole moments of p-NA and
urea while these deviations enhance as one moves from dipole moment to
linear polarizability and nonlinear hyperpolarizabilities. In general,
if we look at the trends, the polarizability values of B3LYP, M06-L,
CAM-B3LYP and HSEH1PBE are relatively large and can be compared with
each other. The dispersion corrected and long-range corrected functional
show more systematic deviations. For instance, among dispersion
corrected functionals, the amplitudes of dipole moments, linear
polarizability and NLO polarizabilities show an increasing trend as ωB97
< ωB97X < ωB97XD < HSEH1PBE. It is also
important to note that LC-B97D and LC-B97D3 of long-range corrected
functional have observed exactly the same values of all the calculated
parameters. Thus, we believe that the current investigation will provide
the benchmark data of reference NLO molecules at different methods for
theoretical community and molecular level insights for experimental
community to design better NLO materials for hi-tech NLO applications.
Suspected drug induced liver injury due to 6-mercaptopurine with a superimposed Sars-Cov-2 infection in a patient with B-ALL
Authors: Cablay Kory , Borowicz Violet , Fulton Ryan
Published: January 2024

6-Mercaptopurine (6-MP) is a chemotherapeutic drug that exhibits
hepatotoxic effects due to its toxic metabolites. This report describes
a case of suspected drug induced liver injury exacerbated by a
Sars-Cov-2 infection. A 16-year-old male with VHR B-ALL was admitted for
hyperbilirubinemia two months after a 6-MP dosage increase and found to
have an active Sars-Cov-2 infection. Liver function improved throughout
hospitalization and the patient was discharged on allopurinol. Following
liver function after a dosage increase of hepatoxic chemotherapy and in
a pediatric oncology patient with an active Sars-Cov-2 infection
undergoing treatment is vital due to potential liver impact.
Establishment of a diagnostic model to distinguish Coronavirus Disease 2019 from influenza A based on laboratory findings
Authors: Xing Dongyang , Tian Suyan , Chen Yukun , Wang Jinmei , Sun Xuejuan , Li Shanji , Xu Jiancheng
Published: January 2024

Background: Coronavirus disease 2019 (COVID-19) and Influenza A are
common disease caused by viral infection. The clinical symptoms and
transmission routes of the two diseases are similar. This study
established a model of laboratory findings to distinguish COVID-19 from
influenza A perfectly. Methods: In this study, 56 COVID-19 patients and
54 influenza A patients were included. Laboratory findings,
epidemiological characteristics and demographic data were obtained from
electronic medical record databases. Elastic network models, followed by
a stepwise logistic regression model were implemented to identify
indicators capable of discriminating COVID-19 and influenza A. Results:
A monogram is diagramed to show the resulting discriminative model. The
majority of hematological and biochemical parameters in COVID-19
patients were significantly different from those in influenza A
patients. In the final model, albumin/globulin, total bilirubin and
erythrocyte specific volume were selected as predictors. This model has
been demonstrated to have a satisfactory predictive performance to
discriminate between COVID-19 and influenza A (AUC=0.844) using an
external validation set. Conclusion: The establishment of a diagnostic
model on laboratory findings is of great significance for the
identification of COVID-19 and influenza A.
LOW RISK PEDIATRIC FOOT SYNOVIAL SARCOMA: PROLONGED OVERALL SURVIVAL WITH AN EXCLUSIVE SURGICAL APPROACH
Authors: Diaz Gabriela Gonzalez , Rossetti Estefania , Innocenti Sergio , Ramos Luz , Marti Jessica Lopez , Rose Adriana
Published: January 2024

Synovial sarcoma (SS) constitutes an aggressive tissue sarcoma that can
affect all age groups but is frequently observed in children and
adolescents. Although a multimodal strategy with surgery, chemotherapy
and radiotherapy tends to be the treatment of choice, adequate risk
stratification has allowed to separate a low-risk group of SS, with long
term survival with an exclusive surgical approach. We report a case of a
pediatric patient with a low-risk foot SS, that achieved prolonged event
free survival and overall survival with exclusive surgical treatment,
minimizing long-term morbidity of chemo and radiotherapy.
Title: A Study on the Stability Analysis of Mathematical Model leading to the Star Formation
Authors: Kumar Sanjeev , Kumar Arun , Kumar Amit
Published: January 2024

Abstract It takes millions of years for a star to get formed. This paper
deals with the stability analysis of mathematical model which governs
star formation. Variation matrix or Jacobian Matrix is developed out of
differential equation which arises from three component of galactic
region i.e. atomic, molecular and stellar components. Mathematical Model
is the best way to explore and explain the system of three components in
the galactic region. In fact, Mathematical Modelling- is the main, if
not the only way one, way for theoretical research of astrophysical
processes and objects [6]. Various examples has been taken for
comparative studies and computational purposes Keywords: Galactic
region, interstellar medium, giant molecular mass, stellar evolution,
galaxy, star formation.
Health status of free-ranging pure and cross-mixed miniature swine population from North East Spain.
Authors: Martorell Jaume , Soler Vicente , Casas Encarna , Closa-Sebastià Francesc , Sanz Albert
Published: January 2024

Miniature pigs have gained popularity as companion animals in the recent
years in Spain. Because the abandonment of miniature pigs and crossing
breeds with wild boars can cause severe problems, investigation about
the health status is needed. Two-hundred and eleven miniature pigs (
Sus scrofa) were included in the study to determine their health
status according to the clinical findings during physical examination
and the results of antibody serology tests against selected infectious
diseases. Their origin, age sex, housing conditions and diet were
recorded. The housing of the animals ranged from wild animals to animals
living in animal sanctuaries. The diet varied from a natural diet in the
wild to commercial and homemade food. Thirty animals out of two-hundred
and eleven were hybrids between miniature pigs and wild boars according
to morphological characteristics. Antibody serology techniques of
Influenza A virus, Hepatitis E virus, brucellosis, tuberculosis, African
swine fever, Classical Swine fever and Aujeszky´s disease was performed.
The prevalence for Influenza A was 5.30%, for Hepatitis E was 5.35%
and the rest tested negative. It is important to control and monitor
these zoonotic infections to prevent Public Health problems. The results
obtained from this investigation demonstrated that the health status of
the animals in this study is optimal and the prevalence of diseases is
similar or minor when compared to previous studies. This study confirms
the hybridization of miniature pig and wild boar in Catalonia.
Knowledge, attitude, and practices regarding COVID-19: A study on food handlers in a joint venture food industry in Dhaka, Bangladesh.
Authors: Jubayer Md. Fahad , Kayshar Md. Shahidullah , Kabir Md. Faizul , Arifin Md. Shoaib , Limon Md. Tariqul Islam , Al-Emran Md. , Uddin Md. Nasir
Published: January 2024

While people around the world are terrified of the global pandemic
coronavirus disease 2019 (COVID-19) and are dying for a permanent
solution, undertaking preventive safety measures are said to be the most
effective way to stay away from it. People’s adherence to these measures
is broadly dependent on their knowledge, attitude, and practices (KAP).
People working in the food industry must be extra cautious during this
time because they are in close proximity to consumable items. The
present study was designed to evaluate food handlers’ knowledge,
attitude, and practices regarding COVID-19 in a food industry in Dhaka,
Bangladesh. A number of 155 food handlers took part in this online-based
study. The information was collected from the participants through a
questionnaire prepared in Google form. With a correct response rate of
about 90% on average (knowledge 89.7%, attitude 93%, practices
88.2%), the participants showed a good level of KAP regarding COVID-19.
However, education and working experiences had a significant association
with the total KAP scores (p < 0.05). Further KAP studies in
different generic food industries in Bangladesh should be carried out to
bring a more precise picture for ensuring the level of workplace and
worker’s safety.
Dissection of physiological, transcriptional, and metabolic traits in two tall fescue genotypes with contrasting drought tolerance
Authors: Kang Yun , Talukder Shyamal , An Zewei , Torres-Jerez Ivone , Krom Nicholas , Huhman David , Udvardi Michael K. , Saha Malay
Published: January 2024

Tall fescue is an important cool-season perennial forage grass that
forms mutualistic symbioses with fungal endophytes. Physiological,
biochemical and transcriptional comparisons were made between two tall
fescue genotypes with contrasting drought tolerance (tolerant, T400, and
sensitive, S279), either with or without endophyte (Epichloë
coenophiala). Drought stress was applied by withholding watering until
plants reached mild, moderate and severe stresses. Physiological
characterization showed that T400 had narrower, thicker leaves, and
lower leaf conductance under well-watered conditions, compared to S279.
After severe drought and recovery, endophytic T400 had greater shoot and
root biomass than other plant types. Under drought, leaf osmotic
pressure increased much more in T400 than S279, consistent with
accumulation of metabolites/osmolytes, especially proline. Gene Ontology
enrichment analysis indicated that T400 had more active organic acid
metabolism than S279 under drought, and implicated the role of endophyte
in stimulating protein metabolism. Transcription factor (TF) binding
motif enrichment analysis of the promoters of drought up-regulated genes
point to important regulatory roles for bZIPs and bHLHs in controlling
such genes, with the core binding motif (C/G)ACGTG being identified. A
much larger variance was observed in TF binding motif enrichment in the
promoters of drought down-regulated genes.
RSV-associated hospitalization in adults in the USA: burden, management strategies and outcomes
Authors: Walsh Edward , Lee Nelson , Sander Ian , Stolper Robert , Zakar Jessica , Wyffels Veronique , Myers David , Fleischhackl Roman
Published: January 2024

Background: The burden of respiratory syncytial virus (RSV) infection in
adults is of growing concern. This study was designed to quantify
disease burden, treatment approaches, and outcomes associated with RSV
infections in adult subpopulations, from pre-hospitalization to hospital
discharge. Methods: A retrospective chart analysis was conducted to
collect patient-case data from hospitalized US adults (aged
>18 years) with RSV infection during two RSV seasons.
Patients were categorized into risk groups: comorbid lung disease,
immunocompromised, older adults (aged ≥65 years), and other adults (aged
<65 years). Physicians reported diagnosis, treatment choices
including respiratory supportive therapy (oxygen and fluid
supplementation), and outcome variables using a standardized online case
form. Results: The majority (277/379; 73%) of patients presented to the
emergency room, with a mean age of 60 years. Once hospitalized, median
length of stay was 6.0 days (3.0-9.0), with disease severity having the
greatest impact on duration of stay. No significant between group
differences in rates of patients requiring management in intensive cares
unit were found (comorbid lung disease, 28%; immunocompromised, 36%;
older adult, 26%; and other adult, 23%). Overall, respiratory
supportive therapy was the most commonly used form of treatment.
Antibiotics were administered in over half of all risk groups (comorbid
lung disease, 61%; immunocompromised, 59%; older adult, 59%; and
other adult, 51%). Patients usually required follow-up visits following
discharge, with 10%-16% requiring skilled nursing care and
approximately 25% requiring assistance from a social worker.
Conclusion: RSV in adult subpopulations, irrespective of age, is a
significant burden to healthcare systems.
Antenatal Detection of Aberrant Subclavian Artery (ARSA) by 4D-Volume Contrast Imaging and High Definition Flow in Mid-trimester Ultrasound Exam
Authors: Aygün Elif , Pata Özlem , Dilek Talat
Published: January 2024

Purpose: To report a small series of ARSA (Aberrant Right Subclavian
Artery) cases which were diagnosed by 4-D (Dimensional) ultrasound
(Volume Contrast Imaging-A Plane) with high definition flow technique.
Method: Clinical data of total 1859 singleton, low risk pregnancies from
the hospital database. Total 13 cases with isolated ARSA cases were
identified. Results: Total 13 cases with isolated ARSA cases were
identified. Median maternal age was 32 years old. Median gestational age
at ultrasound exam was 21 weeks. Eleven of thirteen cases were isolated.
Case 3 had a muscular ventricular septal defect (VSD) and case 6 had an
aberrant left subclavian artery. All cases were detected by 4D VCI-A
with high definition flow technique successfully. One fetus with ARSA
who was misdiagnosed as normal by conventional 2-D ultrasound, 4D VCI-A
with high definition flow revealed abnormal courses of the right
subclavian artery. Course of azygos vein was discriminated from the ARSA
in all cases. Conclusion: 4D VCI-A mode activated with HD-flow (High
Definition) is a valuable tool for the investigation of outflow tracts
of fetal heart and proximal branches of aorta in midtrimester scans and
carries some advantages over conventional 2D sonography combined with
High Definition Flow mode.
VERY EARLY DIAGNOSIS AND MANAGEMENT OF CONGENITAL ERYTHROPOIETIC PORPHYRIA AT BIRTH
Authors: Desjardins Marie-Pier , Naccache Lamia , Hébert Audrey , Auger Isabelle , Teira Pierre , Pelland-Marcotte Marie-Claude
Published: January 2024

Congenital erythropoietic porphyria (CEP) is caused by a defect in the
heme biosynthesis pathway of the enzyme uroporphyrinogen III synthase
(UROS) leading to an accumulation of non-physiological porphyrins. The
exposure of accumulated porphyrins to sunlight causes severe
photosensitivity, chronic intravascular hemolysis and, eventually,
irreversible mutilating deformities. Several supportive therapies such
as strict sun avoidance, physical sunblocks, red blood cells
transfusion, hydroxyurea and splenectomy are commonly used in the
management of CEP. Currently, the only available curative treatment of
CEP is HSCT. We present a child with a very early diagnosis of CEP, who
became the youngest successful HSCT for CEP.
A Finite Difference Approximation for Numerical Simulation of 2D viscous Coupled Burgers equations
Authors: YAGMURLU Nuri , GAGİR Abdulnasır
Published: January 2024

In the present article, the numerical simulation of the two-dimensional
coupled Burgers equation (2D-cBE) has been sought by finite difference
method based on Crank-Nicolson type approximation Widely used three test
examples given with appropriate initial and boundary conditions are used
for the simulation process. During the simulation process,the error
norms L₂, L_{∞} are calculated if the exact solutions are already
known, otherwise the pointwise values and graphics are provided for
comparison. The newly obtained error norms L₂, L_{∞} by the presented
schemes are compared with those of some of the numerical solutions in
the literature. A good consistency and accuracy are observed both by
numerical values and visual illustrations.
Impact of the COVID-19 pandemic on drug-related issues and pharmacist interventions in geriatric acute care units
Authors: Chappe Marion , Corvaisier Mathieu , Brangier Antoine , Lagarce Frédéric , Raimbault-Chupin Mélina , Annweiler Cédric , Spiesser-Robelet Laurence
Published: January 2024

To assess and compare the activity of pharmaceutical analysis on drug
management in a geriatric acute care unit prior to and during the
COVID-19 pandemic. This was a single-centre, observational,
retrospective, and comparative cohort study. All Pharmacist
Interventions (PIs) carried out in the unit between 27 January 2020 and
30 April 2020 were distinguished according to whether they were
conducted prior to or during the first wave of COVID-19. The main
outcome measure was the rate of PIs per patient and per line of
treatment analysed. Other data collected were the drug class managed by
the PI, the Drug Related Problems (DRP) identified, the nature of the
advice given, and the acceptance rate by geriatricians A total of 355
stays were analysed, with PIs generated for 21.7% of the stays prior to
COVID-19, and for 53.4% of the stays during the first wave (p=1.029
E-9). Among the 4,402 lines of treatments analysed, 54 PIs were carried
out for prescriptions prior to COVID-19, and 177 during the first wave
(p=0.002). DRPs were mostly related to anti-infectious drugs during the
pandemic (20.3%, p=0.038), and laxatives prior to the pandemic (13.0%,
p=0.023). The clinical impact of the PIs was mainly moderate (43.7%).
The acceptance rate was 59.3%. A greater amount of DRPs were detected
and more therapeutic advice was proposed during the first wave of
COVID-19, with a focus on drugs used for the management of COVID-19
rather than geriatric routine treatments. The needs for clinical
pharmacists were strengthened during the pandemic.
The role of cooperatives in the process of development of agriculture and integration into trade area of the European Union case of Georgia
Authors:   Batumi Shota Rustaveli State University, Shainidze Ekaterine , Verulidze Vazha ,   Batumi Shota Rustaveli State University, Surmanidze Iamze ,   Batumi Shota Rustaveli State University
Published: January 2024

In terms of agricultural development, Georgia, taking into account its biodiversity and soil and climatic conditions, is a country with significant potential in the Caucasus region. Despite the mentioned natural advantages, Georgia's agriculture has been facing significant challenges, in particular local production is underdeveloped, food safety is not protected and the food self-sufficiency ratio is low. In the context of agricultural development support programs, legislative regulation of cooperative activities and an association agreement signed with the European Union, the development and revival of agriculture was expected, which should have been a prerequisite for the possibility of opening the European Union market for Georgian agricultural products; however, in the context of land fragmentation, it’s still not possible to stimulate the production and export of agricultural products. The aim of the article is to assess the role of cooperatives in the process of development of agriculture and integration into the trade area of the European Union. The main reason for the failure of ongoing reforms in the field of agriculture is its inconsistency and imperfect laws, which leads to the inability of individual state programs to support agricultural value chain. Thus, in order to ensure the success of the reform, it is necessary to stimulate cooperatives using systematic and integrated approaches, which means, first of all, increasing the access of cooperatives to land, providing training services, technological, marketing support, providing preferences in public procurement, ensuring compliance with the conditions of product standardization and continuity of supply to enter the EU market.
Rapid progression of large intracranial cerebral artery involvement in a patient with myeloperoxidase antineutrophil cytoplasmic antibody-associated vasculitis
Authors: Ko Jihee , Choi Jay Chol
Published: January 2024

Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a systemic necrotizing vasculitis that predominantly affects small vessels of the body. The two most common ANCAs are myeloperoxidase ANCA and proteinase 3 ANCA. Neurological manifestations are frequent in patients with AAV, including peripheral neuropathy, meningitis, and stroke. AAV-associated ischemic stroke usually affects small vessels supplying the white matter or brainstem. This case report details the presentation and treatment course of a 70-year-old man with rapidly progressive multiple intracranial large artery involvement attributed to myeloperoxidase ANCA-associated vasculitis. Despite treatment with high-dose steroids and a rituximab infusion, the patient developed new speech difficulties and respiratory distress, and brain imaging confirmed new stroke lesions with progressive multiple intracranial large cerebral artery involvement. The patient died from SARS-CoV-2 infection 4 months after the diagnosis. This case emphasized the rare presentation of rapidly progressive large vessel involvement in a patient with myeloperoxidase ANCA-associated vasculitis despite active immunotherapy.