bluuebunny/crossref_metadata_2025 huggingface dataset:
Designing a Concept for the Content of Journalism Training
Authors: Avdonina Natalia , Kuprikov Nikita , Kuprikov Mikhail
Published: December 2023

This study examined the issue of professional training of future journalists in terms of promoting the formation of their professional identity. The relevance of the research topic is due to trends in the profession represented in the content of professional training. The purpose of the study is to present a draft concept for the professional training of future journalists in terms of the formation of their professional identity. The research findings are presented by the Federal State Educational Standard, professional standards in «Journalism» major at Northern (Arctic) Federal University named after M.V. Lomonosov. The research methodology employed a systematic and constructivist approach, which makes it possible to analyze the system of professional training of future journalists from professional identity aspect. The concept of professional training of future journalists includes: goals (strategic and tactical); conceptual apparatus (professional identity, image of I-professional, image of the profession); characterization of objective factors of the macrosocial context and subjective factors of personality development that influence the formation of the professional identity of future journalists; the content of professional training (courses aimed at mastering the competencies of analytical and narrative journalism); organizational and pedagogical conditions for the formation of the professional identity of future journalists; stages of formation of professional identity of future journalists. As part of the study, we developed a learning methodology in a production studio based on a project-based, reflective and task-based approach and CDIO engineering education technology. The presented concept reflects the formation of a person as a professional, his professional identification through the stages of self-determination (understanding of his place and role in the profession), immersion in professional activities and self-actualization. The results of the study may be useful and interesting for the developers of educational programs in “Journalism” major.
Communication Audit of the Presence (Web Presence) of an Educational Organization Online
Authors: Stepanov Valentin , Ivanov Sergey
Published: December 2023

In this study the authors consider targeted information and communication management as a means of a unique entity of public capital of an educational organization, its web presence (“presence” in the information and telecommunications network “Internet”). The object of the study are state and non-state educational organizations (universities). The purpose of the study is to systematize the promotional communication of the universities and targeted activities in the university to manage them as contributing to the formation of competencies aimed at the effective use of information and communication technologies in the professional activities of specialists. The general scientific methodology of scientific research was developed according to the specifics of the subject and object of the research. In general, in all universities, the management of the information field (web presence) is as a rule systemic, in each university the technological subject of managing information and communication activities of the university is distributed, though there are no traces of coordination efforts within the framework of a distributed subject in order to achieve a synergistic effect. The analysis of the effectiveness of publications in social networks showed that subscribers of accounts in social networks, in general, actively respond to publications, willingly comment on posts (engagement rate), actively share the social values of the university (growth rate), and react emotionally to broadcast messages to a much lesser extent (sympathy coefficient). The results of the study allow optimizing the system of information and communication support for promoting communication in a modern educational organization. The findings can be used in the practice of promoting educational services and allow clarifying the elements of managing the media sphere of a university and the publicity capital of an educational organization.
The Internet of Things as a Promising Channel of Advertising Communication
Authors: Timofeev Vladimir
Published: December 2023

The study explored the future of the advertising industry. In particular, the possibility of a new advertising communication channel associated with the development of Internet of Things technologies is being considered. In his reasoning, the author started from the fact that at present, along with the omnipresence of the Internet, there is a rapid growth of the Internet of Things segment. The further development of the Internet of Things is largely due to the quantitative and qualitative improvement of its physical infrastructure. "Smart" things connected in networks capture and analyze data, exchange information, and take over part of the communications familiar to humans. As a result, new social practices are emerging, including those related to the involvement of connected things in the human decision-making process. A favorable scenario assumes the appearance in the future of many "smart" technologies that will make things even smarter, give them the opportunity to perform a variety of functions and services in the interests of a person. With their help, the interaction of things with things, things with a person can lead to the emergence of completely new forms of interaction that do not fit into the standard framework of ideas about communication.
Based upon the analysis, the author concluded that "smart" structures, which, due to the presence of specific technological and communication parameters, can become a fundamentally new channel of advertising communication and even transform the entire advertising industry. The study examined the features of the model of advertising communication based on the Internet of Things, in which connected physical and virtual things can act as both subjects and objects of advertising communication. The study identified technical solutions that will allow to create the basis for the functioning of advertising communications based upon the Internet of Things. The methods that can accelerate the implementation of the idea of using the Internet of Things as an advertising communication channel were described.
Nucleobase-Modified Microgels Synthesized via Microfabrication Technology for DNA Adsorption
Authors: ÇETİN Kemal
Published: December 2023

DNA isolation is a crucial procedure since DNA-based assays have great importance in molecular biology, biochemistry and biomedical applications. The objective of this study is to fabricate micron-sized hydrogels as adsorbents for DNA. Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microgels were synthesized by free radical polymerization in the presence of N,N'-methylenebisacrylamide as a crosslinker, in the microholes of a microstencil array chip. Then, adenine was immobilized to microgels through the epoxy groups of glycidyl methacrylate. Scanning electron microscopy and Fourier transform infrared spectroscopy were employed to investigate the chemical and morphological characterizations of the microgels. The findings of the experiments demonstrate that the microgels had a cylindrical shape, were of uniform size, and had a height and diameter of around 500 μm. Observation of aromatic C=C peak confirmed the existence of adenine ligand in the microgel structure. Adsorption studies were carried out to determine the optimal conditions for DNA adsorption of nucleobase-immobilized microgels. After initially increasing, the quantity of DNA adsorbed onto the microgels reached a saturation level at a DNA concentration of around 2.0 mg/mL. The maximum adsorption was 38.54 mg/g microgels for an initial DNA concentration of 2.0 mg/mL in the optimum medium pH and temperature. DNA adsorption capabilities are shown to not significantly decline in recurrent adsorption-desorption cycles. As a result of the findings, adenine-immobilized microgels were demonstrated to be a viable option for DNA adsorption. Additionally, as a reference for future research, this study highlights the benefits of microfabrication technology, such as its simplicity of use in fabricating adsorption materials with the desired size, shape, and uniformity.
DToL Specimen Tissue, Blood and Voucher Sampling Standard Operating Procedure: Chordata: Vertebrata: Fish v1
Authors: Januszczak Inez
Published: December 2023

This Standard Operating Procedure (SOP) covers how fish specimens are to be sampled and preserved for vouchering at the Natural History Museum (NHM) in London, in association with the Darwin Tree of Life project (DToL). Please note this SOP covers sampling tissue and blood either during an appropriate veterinary/scientific procedure or post-mortem, with assumed veterinary knowledge. It is applicable if the submitter/centre in question has opportunities to sample tissue from animals that are being either being euthanized for clinical or scientific purposes, undergoing veterinary procedures or eligible biobanked carcasses (storage dependent). NHM prides itself on maintaining the highest quality specimens for vouchering purposes, enabling samples to be used as a reference for any future scientific study. This SOP is covers fish in general - other available Chordata SOPs include DToL Chordata: Vertebrata: Aves and DToL Chordata: Vertebrata (for an overview). This is version one of this SOP, and may be subject to change in the future. Acknowledgements Thank you to Oliver Crimmen at the Natural History Museum for reviewing this SOP.
eDNA extraction from buffer EtOH 100% using DNeasy Blood and Tissue Kit (QIAGEN) v1
Authors: not provided delphine.vanhaecke
Published: December 2023

This is a protocol for extracting eDNA from the buffer EtOH 100% in which Millipore filters are stored.
pHluorin assays, analysis, and fluorescence microscopy v1
Authors: A Parra-Rivas Leonardo
Published: December 2023

pHluorin assays, analysis, and fluorescence microscopy
Protocol for Epidemiological Analysis and Data Visualization in Health v1
Authors: Rodrigues de Oliveira Junior Ronaldo
Published: December 2023

In recent years, information contained in large health databases (big data) has been used in various scientific fields. These databases have been generated from original data that has expanded to encompass not only mortality and survival data but also other dimensions such as healthcare and infection control, becoming a valuable source for conducting epidemiological studies, effectiveness analyses of treatments, and monitoring population health, among other applications. For this purpose, the programming languages R and Python are most commonly used for data processing, statistical analysis, and the creation of graphs and tables. In this context, a protocol was developed using the database of the Sistema de Informação de Agravos de Notificação (SINAN) through the R programming language to create graphs showing the frequencies of comorbidities, symptoms, and a map of the distribution of deaths in the state of Goiás, Brazil, from 2015 to 2022. This script can be used to process and generate figures from other databases.
Attitudes and Barriers of Primary School Children on Cardiopulmonary Resuscitation for Drowning Victims
Authors: Nurumal Mohd. Said , Jamaludin Thandar Soe Sumaiyah , Shamsudin Luqman Aqiel , Ramli Muhammad Zahir
Published: December 2023

Cardiopulmonary Resuscitation is a lifesaving procedure whenever a cardiac arrest victim. Drowning is also one of the factors that can lead to cardiac arrest, especially in coastal areas. Children who live in the coastal community are very at risk for drowning due to the nature of their playground located in the coastal zone. Empowering and educating them to skill on Cardiopulmonary Resuscitation (CPR) is more important to provide effective prevention means in reducing the mortality rate, it would require attitudes and barriers from the public especially. This study aimed to explore attitudes and barriers to CPR in drowning victims for primary school children aged in the coastal community of Malaysia. This study follows a quasi-experimental design with educational videos about CPR pre-and post-intervention. The studied participants were participated in this study through an online google form survey due to the current pandemic situation. All the participants were from primary school children in the coastal area. This study revealed that most of the participants (70.6%) would perform CPR for drowning victims after the intervention given to them compared to pre-intervention, which was 58.8%. As for the “Does the public need to learn CPR?”, most of the participants (88.2%) agreed that everyone needed to learn CPR procedures after giving the intervention. 76.5% of the participant want training on CPR for drowning before the intervention, but there is a slight decrease after intervention given, which is (64.7%). Around 58.8% of the participants were confident in initiating CPR for drowning victims before and after the intervention. Moreover, approximately 82.4% of the participant would perform CPR if their friends were in danger after the intervention. The majority of the participants (70.6%) think adults are more suitable for performing CPR in the post-intervention questionnaire than only 52.9% for the pre-intervention questionnaire. Primary school children showed a significant change in their attitudes and barriers after receiving the intervention. Consideration should be given to integrating the CPR training or lesson in the syllabus of primary education would save more lives.
Audiovisual Media’s Effectiveness on Community Knowledge about First-Aid Accidents
Authors: Fathoni Fajar Ilham , Siwi Adiratna Sekar , Suandika Made , Tang Woung Ru
Published: December 2023

Traffic accident is one of the most significant causes of death globally. Handling accident victims within the first hour is a critical time to save accident victims and avoid bad conditions or death. People who do not provide first aid do not have good knowledge and skills about first aid. Efforts to increase knowledge can be made through counseling. The learning media used in general are two, namely electronic media and print media. This study aim to determine audiovisual media's effectiveness in increasing public knowledge.  The design used pre-experiment one group, pre-test post-test. Instruments used questionnaires and audiovisual media. The samples used were village management and surrounding communities, and sampling using g-power software 3.1 obtained the results of 56 respondents. Data analysis using the Wilcoxon test.  The study showed that public knowledge about first aid measures in accidents before being given audiovisual media good knowledge (73.2%). However, after being given audiovisual press, almost all respondents had significantly increased knowledge in the category of good knowledge (98.2%). There is a significant effect of the effectiveness of the audiovisual method in increasing public understanding of as many as 14 respondents on first aid actions in accidents with a p-value = .0001 ( 0.05).  Public knowledge of first aid is urgently needed to save accident victims. People who already know the importance of community assistance can perform first aid so that injuries do not become severe.
The Impact of COVID-19 Pandemic on Nursing Education among Nursing Students: A Systematic Review
Authors: Jamaludin Thandar Soe Sumaiyah , Nurumal Mohd Said , Hasan Muhammad Kamil Che , Shukor Nadiah Nurulnasuha Abdul , Win Kyu Kyu
Published: December 2023

Globally, the COVID-19 pandemic has had a significant impact on educational sections and is required to close down due to the transmission of the coronavirus.  The newly developed online learning is the most reliable option to resume the education process by preventing the academic calendar from accumulating and minimizing learning loss. This systematic review aimed to search the available literature on the impact of the COVID-19 pandemic on nursing education. Searching the literature was systematically done using the PRISMA flow diagram, and the inclusion and exclusion criteria of the study were followed by the PICO/S framework. The quality assessment was conducted by using the CASP.  30 articles included in this systematic review. Three themes emerged in this review. Those are knowledge and perception of the nursing students towards COVID-19, experience and readiness of the nursing students towards E-learning, and the attitude and psychological impact on the nursing students towards COVID-19.
The Influence of Video Guide to Using the iPosyandu Application on Increasing Recording and Reporting Skills for Cadres in Purwarkarta Regency
Authors: Fabianti Vanny , Rinawan Fedri Ruluwedarata , Arisanti Nita , Fitriana Lisna Anisa , Rohaedi Slamet
Published: December 2023

The iPosyandu application is an innovation in the health sector in the form of mobile technology to assist cadres in recording and reporting. Implement cadres can record and report using iPosyandu. Video as a communication channel for the diffusion of innovations is an attempt to convey messages so that the knowledge, attitudes, and skills of someone who watches the video change. The study aimed to identify the effect of video guides on cadre skill improvement in recording and reporting using the iPosyandu application. The pre-post test study in 120 cadres in Purwakarta Regency was divided into two groups, 60 cadres in the intervention group and 60 in the control group. The intervention group received modules and videos on using the iPosyandu application, while the control group only received modules on using the iPosyandu application. iPosyandu recording and reporting skills using observation sheets. The study shows that video guide influences improving the skills of cadres when using the iPosyandu application (p0.001). Video as a media guide that suits the needs of cadres and media experts can affect the skills of cadres in recording and reporting through iPosyandu.
Síndrome de Cascanueces Posterior: reporte de un caso en pediatría y revisión de la literatura
Authors: Montoya-Montoya Cristina , Serna-Higuita Lina María , Baquero-Rodríguez Richard
Published: December 2023

El síndrome de cascanueces se produce por la compresión de la vena renal izquierda entre la aorta y la arteria mesentérica superior, este síndrome se asocia a síntomas variables e inespecíficos de congestión venosa secundarios. La forma más común del síndrome es la clásica o anterior, pero existen otras variantes atípicas, entre ellas se encuentra el síndrome de cascanueces posterior, el cual ocurre por la compresión de la vena renal izquierda entre la aorta y la columna lumbar. Se describe el caso de una paciente de 5 años con hematuria macroscópica y proteinuria leve persistentes, en la cual se descartó compromiso glomerular y, por lo atípico del cuadro clínico, se realizó angioresonancia magnética abdominal que confirmó el diagnóstico del síndrome de cascanueces posterior.
Afrontamiento familiar en el intento de suicidio: revisión sistemática 2005-2020
Authors: Correa Ramírez Cristian , Martínez Pérez Karina Andrea , Marulanda Saldarriaga Daniel , Vélez Martínez Yojan Steven
Published: December 2023

El intento de suicidio se constituye en un problema de salud pública; su comprensión en el marco de salud mental debe incluir una mirada sistémica que vincule a la familia. El afrontamiento familiar tras dicho evento puede favorecer el bienestar de sus integrantes, o puede allanar nuevos escenarios multiproblemáticos que agudicen la crisis; por esta razón, el objetivo de la presente investigación se enmarca en la revisión sistemática de literatura científica, en torno a la dinámica y al afrontamiento familiar luego del intento de suicidio de uno de sus integrantes. Método: bajo el modelo prisma se llevó a cabo una revisión sistemática de publicaciones entre 2005 y 2020 en torno al tema, a partir de cuatro bases de datos (ESBCOhost,, ScienceDirect, Scopus y Scholar Google), se privilegiaron investigaciones empíricas excluyendo revisiones sistemáticas. Resultados: el afrontamiento familiar puede ser comprendido en el marco de distintas temporalidades que posibilitan un ajuste adecuado o inadecuado para el sobreviviente y su grupo familiar, destacándose reacciones familiares iniciales con alto componente emocional, estrategias de afrontamiento y cambios en la dinámica familiar. Se concluye que el acompañamiento en salud mental debe captar el marco de temporalidades bajo las cuales se presenta el afrontamiento.
O ESTÁGIO CURRICULAR E O DESENVOLVIMENTO DE COMPETÊNCIAS DOS ACADÊMICOS DO CURSO DE ENFERMAGEM
Authors: Miyazawa Ana Paula , Tomaz Jerzuí Mendes Torres , Tavares Carlos Henrique Falcão
Published: December 2023

Este estudo tem como objetivo analisar a percepção dos estudantes de graduação do Curso de Enfermagem sobre as competências gerenciais desenvolvidas no estágio curricular. Trata-se de um estudo descritivo de abordagem qualitativa cujos sujeitos foram 19 estudantes que realizaram estágio curricular do Curso de Enfermagem em uma IES privada localizada no município de Maceió/AL. Os relatos levantados evidenciam a potencialidade do estágio no desenvolvimento de competências gerenciais necessárias à atuação do enfermeiro nos serviços de Atenção Básica. Ao se deparar com acontecimentos reais, o estagiário é impulsionado a refletir sobre as diferentes situações que se apresentam no contexto do trabalho em saúde, conduzindo-o a planejar suas ações para, apenas posteriormente, implementá-las.
IMPACTOS DA EDUCAÇÃO SOBRE A INFORMALIDADE NO ESPÍRITO SANTO E NO SUDESTE DO BRASIL (2016-2021)
Authors: Freislebem da Rocha Antônio Ricardo , Ramalhete Moreira Ricardo , Lira Pablo Silva
Published: December 2023

O objetivo deste trabalho é analisar a relação existente entre a mudança na composição educacional da mão de obra ocupada e seus impactos sobre a dinâmica da informalidade do mercado de trabalho do Espírito Santo e da região Sudeste brasileira entre 2016 e 2021.  Foram utilizados os dados da PNAD-Contínua do IBGE e metodologia de decomposição da informalidade em efeito nível e efeito composição. Os resultados evidenciaram que o efeito composição foi fundamental para evitar um maior crescimento da taxa de informalidade no Espírito Santo e no Sudeste, podendo ainda ser indicativo de uma maior seletividade das empresas ao selecionar os candidatos à vaga de trabalho, uma vez que se observou aumento da informalidade até mesmo entre os mais escolarizados.
Unraveling US Newspapers’ Digital and Print Subscriptions in the Context of Price, 2016–2022
Authors: Chyi Hsiang Iris , Jeong Sun Ho
Published: December 2023

Despite industry-wide efforts in digitally transforming news organizations, research showed that most newspapers’ legacy products still outperformed the same newspaper’s digital offerings in terms of engagement, circulation, readership, pricing, advertising, and subscription revenue—all by a wide margin. But Covid-19 created an unprecedented scenario where the need for instant, local news updates, the fear of contacting anything tangible, and financial stress may have changed consumer behavior. To assess the state of the newspaper industry, this study analyzes short-term and long-term trends in US newspapers’ digital and print circulation before and during the pandemic. The analysis considered price, an important factor often neglected in discussions about newspaper demand. Utilizing rich industry data, this study analyzed 18 US metro daily newspapers’ circulation trends during 2016–2022. The results revealed that digital circulation increased rapidly after the onset of Covid-19 but subsequently decreased after reaching the peak in Q3 2021. Print circulation continued its rapid decline since 2016, accompanied by continuous, substantial price hikes for print subscriptions—a typical print subscription now costs over $1,000 a year. Despite circulation declines, the print edition remains the core product, with more subscribers paying far more than digital subscribers. Because of the immense price gap (6 to 1), the seemingly promising increase in digital subscriptions during Covid-19 could not generate nearly as much revenue to cover the loss on the print side, resulting in a substantial loss in total subscription revenue. The state of the US newspaper industry needs immediate attention.
Results of enzyme immunoassay of vasculoendothelial growth factor (VEGF) in blood serum in premature newborns with perinatal hypoxic damage to the central nervous system
Authors: Golosnaya G. S. , Krasnorutskaya O. N. , Ermolenko N. A. , Kholichev D. A. , Ogurtsov A. V.
Published: December 2023

Background. Vasculoendothelial growth factor (VEGF) is responsible for vascular remodeling, influences the formation of post-hypoxic structural changes in the newborn brain and is synergistically closely related to neurotrophic factors, being an inhibitor of apoptosis processes, which are important for lesions of the central nervous system in newborns of various types of gestational age, having suffered both acute asphyxia at birth and chronic intrauterine hypoxia. VEGF has been little studied in premature newborns, which are at high risk for the formation of intraventricular hemorrhage and periventricular leukomalacia.Aim. To study changes in the serum concentration of VEGF, its role in the pathogenesis of severe hypoxic-ischemic lesions of the central nervous system in premature newborns of various gestational ages, as well as to determine its prognostic significance for the formation of severe structural brain lesions.Materials and methods. We observed 120 children with a gestational age from 25 to 36 weeks. The children were divided into 4 groups according to changes to neurosonography data. Determination of protein level was carried out by enzyme immunoassay.Results and conclusion. With the formation of ischemic and combined (intraventricular hemorrhage and periventricular leukomalacia) forms of post-hypoxic changes in the brain in newborns, by 5–7th days of life the concentration of VEGF significantly decreased compared to the test on the 1st day of life, and by the 4th week of life it decreased in 4 times in case of combined lesions. VEGF cannot be used as a marker of damage in the acute period (up to 5 days of life), since its initial levels in the blood serum do not differ significantly from those in the control group. However, a decrease in its concentration by the end of the 1st week of life makes it possible to reliably predict the formation of post-hypoxic changes in the brain. A decrease in the level of VEGF in the blood serum in premature newborns with structural changes according to neurosonography by the 4th week of life coincides with the timing of the formation of gliotic changes, which significantly affects the developmental prognosis of the examined children
ONE OF THE BUILDERS OF RENAISSANCE VALUES OF UKRAINIAN NATION
Authors:   Pereiaslav Hryhorii Skovoroda State Pedagogical University, Korpaniuk Mykola
Published: December 2023

The creativity of Kasiian (Kallist) Sakovych is the precious treasure in the national Renaissance and classical literature, philosophy, polemical journalism, fraternal and educational movement of the first half of the 17th century. He strived to be as useful as possible for his nation in the European progress, he worked not only on the current topics of the heroic struggle against enemies (poem-panegyric about Petro Sahaidachnyi), scientific latest philosophical interpretations of the doctrine of the soul, Aristotle’s legacy, connections of philosophy and religion. He insistently offered the ways to overcome scholasticism in the Orthodox Christianity, the low level of the reforms in the Greek Catholic Church, his vision of the need for a scientific approach to doctrinal issues, which caused considerable resistance and led Sakovych to transition from Orthodoxy to the union, and then to Roman Catholic Church. The colourful and deeply erudite figure of Kasiian Sakovych, the stage level of his scientific and literary work attracted attention of the medievalist scholars such as M. Maksymovych, M. Hrushevskyi, B. Krysa, P. Kraliuk, Ada and Bohdan Bychky, Valerii Shevchuk. The need of further studies of his legacy is growing, so this article is a small contribution to its understanding.
UKRAINIAN-POLISH FRONTIER: DIALOGUE OF LANGUAGES AND CULTURES
Authors:   Rivne State University of Humanities, Sovtys Nataliia
Published: December 2023

The article is devoted to the phenomenon of Ukrainian-Polish linguistic and cultural frontier. The basis for the emergence of common cultural phenomena and intercultural dialogue are considered. The article substantiates the peculiarities of the choice of terminology for outlining the concepts of “frontier studies”. Fiction, which is biographically or thematically related to the frontier, often reproduces the existing socio-cultural model of the region, which is characterized by polyphony and dialogue. In one literary text, we come across different cultures that speak in different “voices” but seek to establish a dialogue with each other. The multicultural nature of the frontier requires many factors to be taken into account, including the language aspect. As the interpretive material for modeling linguistic and ethnocultural communications in the context of the dichotomy “friend or foe” as well as a number of other aspects, serve the poetic works of L. Węgliński (c. 1827-1905), who wrote in Polish and Ukrainian. Bilingualism of L. Węgliński acts as a kind of artistic optics that determines the poetics and semantics of his works of art, the formation of his “self” in another’s environment, and non-native language becomes a significant stimulus for his work, a kind of tool. Borrowings and quotations from other languages: French, German, Italian and English in works written in Ukrainian and Polish, testify to the author’s polilingualism, and also confirm that the western lands of Ukraine in the late nineteenth – early twentieth century were a developed European region, the population of which was not limited to one language, but used foreign language vocabulary, usual at the time for Western Ukrainian lands. To the representatives of most peoples, which we meet on the pages of the collections, we can apply the intermediate category of “another”, which indicates the cultural acceptance, a certain interaction and its dialogue in the communicative model of the frontier. In the bilingual works of L. Węgliński we observe not only the peculiarities of the author’s own linguistic consciousness, but also the linguistic and cultural specifics of the Ukrainian-Polish frontier, further study of which will contribute to the deepening of the theoretical foundations of the history of Ukrainian and Polish literary languages.
THE ART OF STAINED GLASS BRINGS UKRAINIAN AND POLISH CULTURES TOGETHER (WORKS BY ADAM DOBRZAŃSKI)
Authors:   Orthodox Theological  Academy of  Kyiv, Stepovyk Dmytro
Published: December 2023

The article highlights the unique work of a prominent Polish master of monumental art, the author of numerous stained glass windows, murals and mosaics of the temples of Poland by Adam Stalony-Dobrzanski (1904-1985), whose father Felix Dobrzanski is a Pole, and mother Anna Kovalenko, a native of Chernihiv, Ukrainian. Dobrzanski spent his youth in Ukraine, and in 1923 he moved with his parents to the restored Polish state; He graduated from the Cracow Academy of Arts and began working in the field of artistic design of Catholic churches, Greek Catholic and Orthodox temples. Feature of Dobzhansky’s creative style is a harmonious combination of ancient medieval Gothic style with the latest styles of Impressionism, Modernism and others; as well as the introduction of inscriptions with the original letter form as an integral part of the stained glass image system. Another noticeable feature of the stained glass windows of Adam Steglovy Dobzhan is the harmony of large and small forms. Of course, he is born, as they say, a monumentalist in the arts. There is greatness in his works. The elongated window openings of the Orthodox, Greek Catholic churches and Roman Catholic churches required verticality. Therefore, this architecture is completely approached by long Gothic stained glass figures. But Dobzhansky would not be Dobzhansky if his age were confined to the composition of tall, sometimes tall, figures. In every large and tall form, he “streams” with many small glasses, in which he writes texts in Latin or Cyrillic letters. This flamboyant scattering of small shapes moves and shimmers like a kaleidoscope, enlivening the statics of the stained glass central figure. Here Dobzhansky presents his knowledge of stained glass. Middle Ages in the Catholic countries of the West, which still amaze the beauty of their kaleidoscopic overflows. There is, of course, a contrast between the large in size and these tiny multicolored glasses. This contrast sends the viewer to the baroque, which maximized the contrast in the riot of flexible forms. These forms do not exist in Mr. Adam’s stained-glass windows, but his contrasts reflect the breadth of the search, a master who crafted his unique language in the field of ancient and modern art styles in the ancient and eternally young stained glass art.
HISTORICAL PROSE OF MICHAL TCHAIKOVSKY IN THE CONTEXT OF THE “UKRAINIAN SCHOOL” AESTHETIC SEARCHES
Authors:   Lesia  Ukrainka  Eastern  European  University, Tsolyk Nataliia
Published: December 2023

The article analyses the visions of Polish literary critics, attesting to a certain reception of the life path as well as the comprehension of Michal Tchaikovsky’s works in the context of the Polish Romanticism “Ukrainian school”. The genre-thematic and compositional features of the prose are also outlined, the author’s narrative strategy is analysed, and both ideological and aesthetic writer views and his literary contacts are shown. The significance of his creativity and activity through the implementation of the romantic idea is clarified. Tchaikovsky’s creation of a prose formula for Romantic Ukrainianism, the search for new forms of transferring Ukrainian stereotypes to Polish soil is still poorly understood. Various manifestations of reception and prose typology, M. Tchaikovsky’s artistic skill in the context of the “Ukrainian school” became the subject of the research. The author’s world-views were taken into account while studying Tchaikovsky’s prose which made it possible to systematically outline the problems, poetic and genre features of the works. The embodiment of the romantic ethnic hero features, a look on the Cossacks are expressed both in the works themselves and in the author’s prefaces, comments, notes making up an integral part of the writer’s creative achievements. M. Tchaikovsky is considered the father of a Polish historical novel. Mythologized vision of history and modern Polish-Ukrainian relations are reflected in various prose forms – diary, gavendas (“Gavendas”, “Legends”, “The Poles and Poles Strange Life”), adventure stories (“Wernygora”, “Kirdzhali”, “Stefan Charnetsky”, “Koshovata”, “Ovruchanyn”, “Hetman of Ukraine”), modern stories (“The Gilovs”, “Anna”), pamphlets (“Bulgaria”, “Nemolyaka”, “Bosnia”, “From Hetman Lach Times”). Slavic-Turkish tales of the Danube Cossacks whose manuscripts were burned in Istanbul remain still unknown. The movement of life was the M. Tchaikovsky environment namely a direct action in which one sees scope, confidence, and desire to bring to life all that he dreamed of. The author of “Wernygora” realised his longing for ancient life through literary activity, he dreamed of Ukraine, and created a world he wanted to see around him. The Cossacks, Slavophilism and colourful personalities have become he sources of M. Tchaikovsky’s works. The moment of literary creativity comes in the periods when the social roles of the politician and the soldier have been lost. The paper is another step towards understanding the place and role of M. Tchaikovsky’s works in Polish romanticism through the prism of historical prose. It is an attempt to read the works scientifically in the context of historical, social and cultural factors at the theoretical level. Research is a contribution to the set of theoretical knowledge of hermeneutics, theory of literature, social anthropology.
THE CATEGORY OF AUTOBIOGRAPHICAL PLACE IN MICHAŁ GŁOWIŃSKI’S PROSE: MEMORY, IMAGINATION, SPACE
Authors:   University of Białystok, Pilichiewicz Kamil
Published: December 2023

The article is devoted to the analysis of Michał Głowiński’s memories of prose using the category of autobiographical place. The author of this term, Małgorzata Czermińska, proposes a new interpretative quality within geopoetics. In the article „Autobiographical places. The geopoetics proposal” details and discusses in turn the types of autobiographical sites referring to various examples. Czermińska’s concept is confronted with Głowiński’s prose works. Places and spaces turn out to be an important element of the memory prose of the author of „Circles of Strangeness”, and the category itself is a valuable tool that can be used to interpret it.
Methods of Developing Student Personal Intellect Through Music Listening Activities in Music Culture Lessons
Authors: Karimova Dildora Amvarovna
Published: December 2023

We know that one of the most important tasks facing education today is the development and formation of the student's intellect. It is possible to perform this task in every direction of education. In this article, we will talk about the theoretical and practical foundations of the development of the student's intellect through the activity of listening to music in music lessons.
Approaches for the protection of Solanum tuberosum from late blight through the regulation of inf1 and inf4 elicitin genes
Authors: Ivanov Artemy A. , Burlakov Alexander V. , Golubeva Tatiana S.
Published: December 2023

Late blight is a disease affecting economically important crops, which is caused by the oomycete Phytophthora infestans. Mainly, fungicides are used against it, however they may harm the environment when used in large quantities. Spray-induced gene silencing (SIGS) can become an alternative to the classical fungicides in the fight against P. infestans. SIGS involves the treatment of plants with double-stranded RNA (dsRNA) which triggers the RNA interference mechanism to suppress translation of the target gene. So, it is possible to suppress the expression of genes.

We have chosen two P. infestans genes, inf1 and inf4 involved in the different stages of the parasite development. For the production of dsRNA in E. coli HT115 two expression vectors were constructed on the basis of the L4440 plasmid, each carrying a cDNA fragment of these genes between two T7 phage promoters oriented in the opposition to each other.

To evaluate the protective effect of the dsRNA potato explants were treated with a solution containing dsRNA of one of the genes or their combination. 24 hours later, phytophthora zoospores were inoculated with the registration of the effect after 5 days.

According to the measurement results, the lesion area was significantly larger in plants treated with water than in the other three experimental groups that were treated with dsRNA.
Overexpression of the MtCLE35 gene in transgenic Medicago truncatula plants inhibits nodulation at early stages of symbiosis development
Authors: Lebedeva Maria A. , Dobychkina Daria A. , Kochetkova Lilia A. , Lutova Lyudmila A.
Published: December 2023

CLE (CLAVATA3/ENDOSPERM SURROUNDING REGION-related) peptides are known as systemic regulators of legume-rhizobium symbiosis that negatively control the number of nitrogen-fixing nodules. These regulatory peptides are produced in the root in response to inoculation with rhizobia, and are transported through the xylem to the shoot, where they are recognized by their receptor, CLV1-like (CLAVATA1-like) kinase, active in leaf phloem cells. After that, a shoot-derived signaling pathway is activated that inhibits subsequent nodule development in the root. Previously, we found that in Medicago truncatula, the expression of the MtCLE35 gene is activated in response to rhizobia and nitrate treatment, and its overexpression systemically inhibits nodulation. However, little is known about the downstream target genes regulated by a MtCLE35 signaling pathway in the root. Moreover, it is not completely clear which stage of symbiosis development is affected by MtCLE35-activated pathway. In order to identify genes regulated by the MtCLE35-induced signaling pathway, we performed a transcriptomic analysis of the roots overexpressing the MtCLE35 gene. Totally, 1122 genes were found to be differentially expressed between MtCLE35-overexpressing and control roots after rhizobial inoculation, among them 185 genes were upregulated and 937 genes were downregulated. Among downregulated genes, many known regulators of legume-rhizobia symbiosis were found. In addition to this, we analyze early steps of interaction between M. truncatula overexpressing the MtCLE35 gene and Sinorhizobium meliloti labeled with fluorescent reporter. We did not observe penetration of S. meliloti into host plant roots with MtCLE35 overexpression. Our data suggest that overexpression of the MtCLE35 gene inhibits nodulation at the very early stages of symbiosis development.
Genetic enhancement of Datura metel for optimized silver nanoparticle synthesis
Authors: Meenakshi S. , Delta A. , Kaushik P.
Published: December 2023

The burgeoning field of nanotechnology has witnessed a surge in the utilization of biological entities, especially plant extracts, for the green synthesis of nanoparticles. In this innovative study, we have ventured into the realm of genetic engineering to optimize the synthesis of silver nanoparticles (AgNPs) usingDaturametel, a plant traditionally known for its rich phytoconstituents [1, 2].
Our initial experiments with non-modifiedDatura metelfruit extracts as reducing agents yielded AgNPs with an average size of 40–50 nm, confirmed spectrophotometrically with a peak at 460 nm. Recognizing the potential to enhance this process, we genetically modifiedDatura metelplants to amplify their phytoconstituent content by approximately 30%. This was achieved by overexpressing genes associated with the production of specific phytochemicals, such as polyphenols and amides.
Subsequent synthesis processes using the GMDatura metelextracts resulted in a 25% increase in nanoparticle yield. Furthermore, the average size of the nanoparticles synthesized from GM extracts ranged between 20–30 nm, indicating a more uniform and refined synthesis process. Advanced analytical techniques, including X-ray diffraction, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and Energy-dispersive X-ray spectroscopy (EDX), were employed to validate these findings. Notably, the EDX analysis of nanoparticles synthesized from GM extracts showcased a silver peak contributing to 32–35% of the weight, a slight increase from the non-modified counterparts.
Beyond the quantitative enhancements, the GM approach also influenced the qualitative properties of the AgNPs. Preliminary tests indicate that the nanoparticles derived from GM extracts exhibit enhanced antimicrobial and antioxidant properties, making them potential candidates for various biomedical applications.
In conclusion, this study underscores the immense potential of integrating genetic engineering with nanotechnology. By genetically enhancingDatura metel, we have not only optimized the synthesis process of AgNPs but also broadened the horizons for their potential applications. However, as we advance in this direction, it is imperative to tread with caution, ensuring the ethical and safe use of GM organisms in research and applications.
Natural transformants of Camellia section Thea
Authors: Chen Ke , Zhurbenko Peter M. , Danilov Lavrentii G. , Matveeva Tatiana V. , Otten Léon
Published: December 2023

Horizontal gene transfer (HGT) plays an important role in plant evolution and plant development. Agrobacterium-mediated gene transfer leads to the formation of crown galls or hairy roots, due to expression of transferred T-DNA genes. Spontaneous regeneration of transformed cells can produce natural transformants carrying cellular T-DNA (cT-DNA) sequences of bacterial origin. HGT from Agrobacterium to dicots is remarkably widespread. The production of naturally genome modified plants could play a role in plant evolution and environment.
Among these natural GMOs (nGMOs) there are the tea plants. Camellia sinensis var. sinensis cv. Shuchazao contains a single 5.5 kb cT-DNA fragment organized as imperfect inverted repeat with three inactive genes. 142 Camellia accessions, belonging to 10 of 11 species of the section Thea, were studied for the presence of cT-DNA alleles. All of them contain the cT-DNA insert, indicating that they are resulted from the single transformed event. Allele phasing showed that 82 accessions were heterozygous for T-DNA alleles, 60 others were homozygous. A phylogenetic analysis of all found alleles showed existence of two separate groups of them, further divided into subgroups. The alleles of the different Camellia species were distributed mosaically over groups, and different species showed very similar T-DNA alleles. This indicates that the taxonomy of Thea requires revision. The nucleotide divergence of the imperfect cT-DNA repeats indicates that the age of cT-DNA insertion is about 15 mya, which is earlier then emergence of section Thea [1]. We present a working model for the origin and evolution of nGMO plants derived from allogamous transformants.
Advancing gene editing: multiplex mutagenesis in hexaploid triticale
Authors: Miroshnichenko Dmitriy N. , Timerbaev Vadim R. , Divashuk Mikhail G. , Pushin Aleksandr S. , Alekseeva Valeria , Kroupin Pavel Yu. , Bazhenov Mikhail S. , Samarina Maria A. , Ermolaev Aleksey , Karlov Gennady , Dolgov Sergey V.
Published: December 2023

The presence of several sets of chromosomes in polyploid crops is a serious problem for the application of gene and genome editing systems. Efficient CRISPR/Cas-based mutagenesis of series of genes involved in the grain starch biosynthesis of hexaploid triticale has been developed. Triticale (×Triticosecale),is a hybrid of rye (Secale) and wheat (Triticum) and consists of three subgenomes. Four genes were targeted and to ensure efficient editing of all subgenomes, a trio of guide RNAs for each target genes were designed. To enable simultaneous editing of 36 genetic loci at once (three sgRNAs × four genes × three subgenomes), an expression cassette was constructed, assembled as an array of twelve sgRNAs. The polysitron vector was delivered to morphogenic calli using a gene gun [1] together with a vector encoding Cas9 nuclease [2] to induce mutations. A number of transgenic plants of spring and winter triticale carrying both Cas9 and sgRNAs inserts have been generated. The efficiency of native gene editing varied depending on the target gene and sgRNA activity. Using a trio of sgRNAs for each target gene, we successfully mutated all three subgenome copies, thereby modifying seed starch synthesis. It can be expected that the described approach will make an important contribution to the future breeding of polyploid crops to produce various combinations of new genetic alleles encoding desired traits.
The study is supported by Kurchatov Genomics Center of All-Russia Research Institute of Agricultural Biotechnology, agreement No. 075-15-2019-1667.