bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical Study of the Lift Enhancement Mechanism of Circulation Control in Transonic Flow
Authors: Chen Ye , Hou Zhongxi , Deng Xiaolong , Guo Zheng , Shao Shuai , Xu Boting
Published: October 2021

The lift of an aircraft can be effectively enhanced by circulation control (CC) technology at subsonic speeds, but the efficiency at transonic speeds is greatly decreased. The underlying mechanism of this phenomenon is not fully understood. In this study, Reynolds averaged Navier—Stokes simulation with k−ω shear stress transport model was utilized to investigate the mechanism of lift enhancement by CC in transonic flow. For validation, the numerical CC results were compared with the NASA experimental data obtained for transonic CC airfoil. Thereafter, the RAE2822 airfoil was modified with a Coanda surface. The lift enhancement effects of CC via steady blowing with different momentum coefficients were tested at Ma=0.3 and 0.8 at α=3∘, and various fluid mechanics phenomena were investigated. The results indicate that the flow structure of the CC jet is insensitive to the incoming flow conditions because of the similarity to the local static pressure field around the trailing edge of the airfoil. Owing to the appearance of shockwaves on the airfoil surface in the transonic regime, the performance of the CC jet is restricted to the trailing edge of the airfoil. Transonic CC achieved a slight improvement in aerodynamic performance owing to a favorable shift in the shockwave pattern and accelerated flow in the separation region on the airfoil surfaces. Revealing the mechanism of lift enhancement of CC in the transonic regime can facilitate the rational design of new fluidic actuators with high activity and expand the potential applications of CC technology.
An Efficient Approach for Predicting Resonant Response with the Utilization of the Time Transformation Method and the Harmonic Forced Response Method
Authors: Zhang Xiaojie , Wang Yanrong , Jiang Xianghua
Published: October 2021

Resonant response of turbomachinery blades can lead to high cycle fatigue (HCF) if the vibration amplitudes are significant. Therefore, the dangerousness assessment of the resonance crossing is important. It requires accurate predictions of the aerodynamic excitation, damping, and response, which will consume immense computational costs. The novel aspect of this study is the development of an efficient approach, which incorporates the time transformation (TT) method to predict the aerodynamic excitations and the harmonic forced response method to obtain the response levels. The efficiency and accuracy of this method were evaluated by comparing with traditional methods for the resonance crossing excited by upstream wake in a 1.5 multistage compressor. For the aerodynamic excitation, discrepancies of ±2% at the mean pressure and ±25% at the harmonic pressure in most areas expect for the blade root were observed, but the calculation time required by the TT method was only 5% of that by the time-marching method. Moreover, response levels with the same aerodynamic forces were compared between the harmonic forced-response and transient dynamic methods. Small differences in the displacement and stress variables were observed; the relative deviation was smaller than 2% with only 1% computing time compared with the transient method, indicating the high accuracy and efficiency of the efficient approach.
Polyglutamine Ataxias: Our Current Molecular Understanding and What the Future Holds for Antisense Therapies
Authors: McIntosh Craig S. , Li Dunhui , Wilton Steve D. , Aung-Htut May T.
Published: October 2021

Polyglutamine (polyQ) ataxias are a heterogenous group of neurological disorders all caused by an expanded CAG trinucleotide repeat located in the coding region of each unique causative gene. To date, polyQ ataxias encompass six disorders: spinocerebellar ataxia types 1, 2, 3, 6, 7, and 17 and account for a larger group of disorders simply known as polyglutamine disorders, which also includes Huntington’s disease. These diseases are typically characterised by progressive ataxia, speech and swallowing difficulties, lack of coordination and gait, and are unfortunately fatal in nature, with the exception of SCA6. All the polyQ spinocerebellar ataxias have a hallmark feature of neuronal aggregations and share many common pathogenic mechanisms, such as mitochondrial dysfunction, impaired proteasomal function, and autophagy impairment. Currently, therapeutic options are limited, with no available treatments that slow or halt disease progression. Here, we discuss the common molecular and clinical presentations of polyQ spinocerebellar ataxias. We will also discuss the promising antisense oligonucleotide therapeutics being developed as treatments for these devastating diseases. With recent advancements and therapeutic approvals of various antisense therapies, it is envisioned that some of the studies reviewed may progress into clinical trials and beyond.
Genomic Sub-Classification of Ovarian Clear Cell Carcinoma Revealed by Distinct Mutational Signatures
Authors: Oliveira Douglas V. N. P. , Schnack Tine H. , Poulsen Tim S. , Christiansen Anne P. , Høgdall Claus K. , Høgdall Estrid V.
Published: October 2021

Ovarian clear cell carcinoma (OCCC) is characterized by dismal prognosis, partially due to its low sensitivity to standard chemotherapy regimen. It is also well-known for presenting unique molecular features in comparison to other epithelial ovarian cancer subtypes. Here, we aim to identify potential subgroups of patients in order to (1) determine their molecular features and (2) characterize their mutational signature. Furthermore, we sought to perform the investigation based on a potentially clinically relevant setting. To that end, we assessed the mutational profile and genomic instability of 55 patients extracted from the Gynecologic Cancer Database (DGCD) by using a panel comprised of 409 cancer-associated genes and a microsatellite assay, respectively; both are currently used in our routine environment. In accordance with previous findings, ARID1A and PIK3CA were the most prevalent mutations, present in 49.1% and 41.8%, respectively. From those, the co-occurrence of ARID1A and PIK3CA mutations was observed in 36.1% of subjects, indicating that this association might be a common feature of OCCC. The microsatellite instability frequency was low across samples. An unbiased assessment of signatures identified the presence of three subgroups, where “PIK3CA” and “Double hit” (with ARID1A and PIK3CA double mutation) subgroups exhibited unique signatures, whilst “ARID1A” and “Undetermined” (no mutations on ARID1A nor PIK3CA) subgroups showed similar profiles. Those differences were further indicated by COSMIC signatures. Taken together, the current findings suggest that OCCC presents distinct mutational landscapes within its group, which may indicate different therapeutic approaches according to its subgroup. Although encouraging, it is noteworthy that the current results are limited by sample size, and further investigation on a larger group would be crucial to better elucidate them.
Activation of Vitamin D Receptor Pathway Enhances Differentiating Capacity in Acute Myeloid Leukemia with Isocitrate Dehydrogenase Mutations
Authors: Sabatier Marie , Boet Emeline , Zaghdoudi Sonia , Guiraud Nathan , Hucteau Alexis , Polley Nathaniel , Cognet Guillaume , Saland Estelle , Lauture Laura , Farge Thomas , Sahal Ambrine , Pancaldi Vera , Chu-Van Emeline , Castelli Florence , Bertoli Sarah , Bories Pierre , Récher Christian , Boutzen Héléna , Mansat-De Mas Véronique , Stuani Lucille , Sarry Jean-Emmanuel
Published: October 2021

Relapses and resistance to therapeutic agents are major barriers in the treatment of acute myeloid leukemia (AML) patients. These unfavorable outcomes emphasize the need for new strategies targeting drug-resistant cells. As IDH mutations are present in the preleukemic stem cells and systematically conserved at relapse, targeting IDH mutant cells could be essential to achieve a long-term remission in the IDH mutant AML subgroup. Here, using a panel of human AML cell lines and primary AML patient specimens harboring IDH mutations, we showed that the production of an oncometabolite (R)-2-HG by IDH mutant enzymes induces vitamin D receptor-related transcriptional changes, priming these AML cells to differentiate with pharmacological doses of ATRA and/or VD. This activation occurs in a CEBPα-dependent manner. Accordingly, our findings illuminate potent and cooperative effects of IDH mutations and the vitamin D receptor pathway on differentiation in AML, revealing a novel therapeutic approach easily transferable/immediately applicable to this subgroup of AML patients.
Artificial Intelligence in Gastric Cancer: Identifying Gastric Cancer Using Endoscopic Images with Convolutional Neural Network
Authors: Islam Md. Mohaimenul , Poly Tahmina Nasrin , Walther Bruno Andreas , Lin Ming-Chin , Li Yu-Chuan (Jack)
Published: October 2021

Gastric cancer (GC) is one of the most newly diagnosed cancers and the fifth leading cause of death globally. Identification of early gastric cancer (EGC) can ensure quick treatment and reduce significant mortality. Therefore, we aimed to conduct a systematic review with a meta-analysis of current literature to evaluate the performance of the CNN model in detecting EGC. We conducted a systematic search in the online databases (e.g., PubMed, Embase, and Web of Science) for all relevant original studies on the subject of CNN in EGC published between 1 January 2010, and 26 March 2021. The Quality Assessment of Diagnostic Accuracy Studies-2 was used to assess the risk of bias. Pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were calculated. Moreover, a summary receiver operating characteristic curve (SROC) was plotted. Of the 171 studies retrieved, 15 studies met inclusion criteria. The application of the CNN model in the diagnosis of EGC achieved a SROC of 0.95, with corresponding sensitivity of 0.89 (0.88–0.89), and specificity of 0.89 (0.89–0.90). Pooled sensitivity and specificity for experts endoscopists were 0.77 (0.76–0.78), and 0.92 (0.91–0.93), respectively. However, the overall SROC for the CNN model and expert endoscopists was 0.95 and 0.90. The findings of this comprehensive study show that CNN model exhibited comparable performance to endoscopists in the diagnosis of EGC using digital endoscopy images. Given its scalability, the CNN model could enhance the performance of endoscopists to correctly stratify EGC patients and reduce work load.
Gallbladder Cancer: Current Insights in Genetic Alterations and Their Possible Therapeutic Implications
Authors: Kuipers Hendrien , de Bitter Tessa J. J. , de Boer Marieke T. , van der Post Rachel S. , Nijkamp Maarten W. , de Reuver Philip R. , Fehrmann Rudolf S. N. , Hoogwater Frederik J. H.
Published: October 2021

Due to the fast progression in molecular technologies such as next-generation sequencing, knowledge of genetic alterations in gallbladder cancer (GBC) increases. This systematic review provides an overview of frequently occurring genetic alterations occurring in GBC and their possible therapeutic implications. A literature search was performed utilizing PubMed, EMBASE, Cochrane Library, and Web of Science. Only studies reporting genetic alterations in human GBC were included. In total, data were extracted from 62 articles, describing a total of 3893 GBC samples. Frequently detected genetic alterations (>5% in >5 samples across all studies) in GBC for which targeted therapies are available in other cancer types included mutations in ATM, ERBB2, and PIK3CA, and ERBB2 amplifications. High tumor mutational burden (TMB-H) and microsatellite instability (MSI-H) were infrequently observed in GBC (1.7% and 3.5%, respectively). For solid cancers with TMB-H or MSI-H pembrolizumab is FDA-approved and shows an objective response rates of 50% for TMB-H GBC and 41% for MSI-H biliary tract cancer. Only nine clinical trials evaluated targeted therapies in GBC directed at frequently altered genes (ERBB2, ARID1A, ATM, and KRAS). This underlines the challenges to perform such clinical trials in this rare, heterogeneous cancer type and emphasizes the need for multicenter clinical trials.
Therapeutic Approaches for Patients with Cystic Fibrosis Not Eligible for Current CFTR Modulators
Authors: Fajac Isabelle , Sermet Isabelle
Published: October 2021

Cystic fibrosis is a severe autosomal recessive disorder caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene encoding the CFTR protein, a chloride channel expressed in many epithelial cells. New drugs called CFTR modulators aim at restoring the CFTR protein function, and they will benefit many patients with cystic fibrosis in the near future. However, some patients bear rare mutations that are not yet eligible for CFTR modulators, although they might be amenable to these new disease-modifying drugs. Moreover, more than 10% of CFTR mutations do not produce any CFTR protein for CFTR modulators to act upon. The purpose of this review is to provide an overview of different approaches pursued to treat patients bearing mutations ineligible for CFTR modulators. One approach is to broaden the numbers of mutations eligible for CFTR modulators. This requires developing strategies to evaluate drugs in populations bearing very rare genotypes. Other approaches aiming at correcting the CFTR defect develop new mutation-specific or mutation-agnostic therapies for mutations that do not produce a CFTR protein: readthrough agents for nonsense mutations, nucleic acid-based therapies, RNA- or DNA-based, and cell-based therapies. Most of these approaches are in pre-clinical development or, for some of them, early clinical phases. Many hurdles and challenges will have to be solved before they can be safely translated to patients.
Epidemiology and Trends over Time of Foreign Body Injuries in the Pediatric Emergency Department
Authors: Ocagli Honoria , Azzolina Danila , Bressan Silvia , Bottigliengo Daniele , Settin Elisabetta , Lorenzoni Giulia , Gregori Dario , Da Dalt Liviana
Published: October 2021

This paper presents the epidemiology of foreign body injuries in the Pediatric Emergency Department (PED) of Padova (Italy) along with its trends over an eleven-year period based on administrative data. Annual incidence rates (IRs) of PED presentations for foreign body (FB) injuries per 1000 person-years were calculated. Univariable and multivariable generalized linear (GLM) Poisson models were estimated to evaluate the relationship between FB injury incidence and year, triage priority, nationality, injury site, and FB type. During the study period, there were 217,900 presentations of pediatric residents in the province of Padova; of these, 3084 (1.5%) reported FB injuries involving the ears, nose, throat, gastrointestinal tract or eyes. The annual IR of FB injury episodes increased from 10.45 for 1000 residents in 2007 (95% CI, 9.24, 11.77) to 12.66 for 1000 residents in 2018 (95% CI, 11.35, 14.08). Nonfood items were the FBs that were most frequently reported. The intermediate urgent triage code was the most represented for FB injuries, with IRs ranging from 5.44 (95% CI: 4.59, 6.40) in 2008 to 8.56 in 2018 (95% CI: 7.50, 9.74). A total of 170 patients who presented for FB injuries were hospitalized (5.5%). The annual FB-related injury IR has increased over time, although most episodes are not life threatening. Educational and prevention programs on FB-related injuries should be promoted and dedicated to childcare providers.
The Effect of Red Mud on Sintering Processes and Minerals of Portland Cement for Roads
Authors: Wang Xiao , Sun Ke , Li Xin , Ma Juntao , Luo Zhongtao
Published: October 2021

As a solid waste generated in the alumina industry, red mud poses a significant environmental hazard and a storage problem. In this study, red mud was added to road cement clinker in order to utilize it. The sintering red mud was first de-alkalized, and then mixed with fly ash, clay, limestone, and sandstone, among other materials, to make Portland cement for road clinker. The effect of the addition of red mud on the thermal decomposition characteristics of Portland cement for roads was studied. The existent states of alkali and radioactive elements in Portland cement for road clinker were investigated by XRD and SEM analysis. The research results showed that the addition of red mud in Portland cement for road raw material significantly promoted the decomposition of carbonates in raw material. The major mineral phases of Portland cement for road clinker were C3S with a polyhedral morphology, quasi-spherical C2S, and tubular C4AF. A small part of the alkali combined with the silicate phase to form a solid solution, and most of the alkali combined with S to form vermiform sulfate in the intermediate phase. The radionuclide 226Ra was mainly distributed in the silicate phase. 232Th was mainly distributed in interstitial phases and then silicate phases, while 40K was mainly distributed in the interstitial phases.
Lobster Distribution and Biodiversity on the Continental Shelf of Brazil: A Review
Authors: Cruz Raul , T. Torres Marina , Santana João V. M. , Cintra Israel H. A.
Published: October 2021

The continental shelf of Brazil is home to a wide range of lobster species, with varying body size, color, habitat preference, and geographic and bathymetric distribution. Spiny lobsters (Panulirus) and slipper lobsters (Scyllaridae) are exploited for export and for the domestic market. Deep sea lobsters (Nephropidae and Polychelidae) have no commercial potential, and little is known about their biology. In this review, we identified 24 lobster species from benthic ecosystems off Brazil (Palinuridae 25%, Scyllaridae 29%, Nephropidae 25%, Polychelidae 17%, Enoplometopidae 4%). We designed a simplified theoretical scheme to understand the role of lobsters in the ecosystem, based on available evidence of distribution, biodiversity, life cycle, connectivity, and abundance. Finally, we propose a theoretical scheme of trophic top-down control, with interactions between a large decapod (spiny lobster), a demersal predator (red snapper), an apex predator (small tuna), benthic invertebrates and fishing exploitation.
Associations of Exposure to Nitrogen Oxides with Prevalent Asthma and Other Atopic Diseases in Israel
Authors: Lev Bar-Or Ruth , Levy Ilan , Twig Gilad , Broday David M. , Lyubarsky Andrey , Derazne Estela , Tzur Dorit , Simon Ben , Levin Noam , Gileles-Hillel Alex , Bentur Lea , Raz Raanan
Published: October 2021

Childhood exposure to nitrogen oxides (NOx) is considered a risk factor for the onset of asthma. However, associations of this exposure with other atopic diseases and factors that modify this association are less clear. We aimed to study associations between exposure to NOx and the prevalence of atopic diseases in Israeli adolescents using a cross-sectional design. The study population comprised all Israeli-born adolescents whose medical status was evaluated for mandatory military recruitment during 1967–2017 (n = 2,523,745), of whom 5.9% had prevalent asthma. We based the exposure assessments on a land-use regression model and estimated associations using multivariable logistic regression models. Across all periods, mean exposure to NOx from birth to adolescence was associated with prevalent asthma at the examination in a dose-response manner, with an odds ratio for the upper quintile of 1.61 (95% CI: 1.56–1.67), in comparison to the lowest quintile. Associations were stronger in males and in lower socioeconomic strata. We found the strongest associations for asthma with comorbid rhinitis, with an almost twofold increase in the odds of upper versus lower quintile of exposure (odds ratio = 1.96, 95% CI: 1.82–2.11). Rhino-conjunctivitis and allergic atopic dermatitis suggested a possible threshold level with NOx. Capsule Summary: Research indicates that half of the global population will suffer from an allergic condition at some point in life. Childhood exposure to nitrogen oxides is a risk factor for the onset of asthma. The association between exposure and allergic diseases other than asthma is unclear. We demonstrate a strong, dose-response relationship between exposure and a group of allergic outcomes, using data comprising 2.5 million subjects over 50 years. The large health benefits from clean air should motivate governments to prioritize mitigation measures.
Surface Detection of Solid Wood Defects Based on SSD Improved with ResNet
Authors: Yang Yutu , Wang Honghong , Jiang Dong , Hu Zhongkang
Published: October 2021

Due to the lack of forest resources in China and the low detection efficiency of wood surface defects, the output of solid wood panels is not high. Therefore, this paper proposes a method for detecting surface defects of solid wood panels based on a Single Shot MultiBox Detector algorithm (SSD) to detect typical wood surface defects. The wood panel images are acquired by an independently designed image acquisition system. The SSD model included the first five layers of the VGG16 network, the SSD feature mapping layer, the feature detection layer, and the Non-Maximum Suppression (NMS) module. We used TensorFlow to train the network and further improved it on the basis of the SSD network structure. As the basic network part of the improved SSD model, the deep residual network (ResNet) replaced the VGG network part of the original SSD network to optimize the input features of the regression and classification tasks of the predicted bounding box. The solid wood panels selected in this paper are Chinese fir and pine. The defects include live knots, dead knots, decay, mildew, cracks, and pinholes. A total of more than 5000 samples were collected, and the data set was expanded to 100,000 through data enhancement methods. After using the improved SSD model, the average detection accuracy of the defects we obtained was 89.7%, and the average detection time was 90 ms. Both the detection accuracy and the detection speed were improved.
Impact of Gene Flow and Introgression on the Range Wide Genetic Structure of Quercus robur (L.) in Europe
Authors: Degen Bernd , Yanbaev Yulai , Mader Malte , Ianbaev Ruslan , Bakhtina Svetlana , Schroeder Hilke , Blanc-Jolivet Celine
Published: October 2021

As for most other temperate broadleaved tree species, large-scale genetic inventories of pedunculate oak (Quercus robur L.) have focused on the plastidial genome, which showed the impact of post-glacial recolonization and manmade seed transfer. However, how have pollen mediated gene flow and introgression impacted the large-scale genetic structure? To answer these questions, we did a genetic inventory on 1970 pedunculate oak trees from 197 locations in 13 European countries. All samples were screened with a targeted sequencing approach on a set of 381 polymorphic loci (356 nuclear SNPs, 3 nuclear InDels, 17 chloroplast SNPs, and 5 mitochondrial SNPs). In a former analysis with additional 1763 putative Quercus petraea trees screened for the same gene markers we obtained estimates on the species admixture of all pedunculate oak trees. We identified 13 plastidial haplotypes, which showed a strong spatial pattern with a highly significant autocorrelation up to a range of 1250 km. Significant spatial genetic structure up to 1250 km was also observed at the nuclear loci. However, the differentiation at the nuclear gene markers was much lower compared to the organelle gene markers. The matrix of genetic distances among locations was partially correlated between nuclear and organelle genomes. Bayesian clustering analysis revealed the best fit to the data for a sub-division into two gene pools. One gene pool is dominating the west and the other is the most abundant in the east. The western gene pool was significantly influenced by introgression from Quercus petraea in the past. In Germany, we identified a contact zone of pedunculate oaks with different introgression intensity, likely resulting from different historical levels of introgression in glacial refugia or during postglacial recolonization. The main directions of postglacial recolonization were south to north and south to northwest in West and Central Europe, and for the eastern haplotypes also east to west in Central Europe. By contrast, the pollen mediated gene flow and introgression from Q. petraea modified the large-scale structure at the nuclear gene markers with significant west–east direction.
The Ideas of Sustainable and Green Marketing Based on the Internet of Everything—The Case of the Dairy Industry
Authors: Nozari Hamed , Szmelter-Jarosz Agnieszka , Ghahremani-Nahr Javid
Published: October 2021

The use of advanced computer technologies has dramatically changed marketing. Concepts such as smart, sustainable, and green marketing have emerged in the last 20 years. One of these new technologies is the Internet of Things (IoT), which has led to the development of the activities and performances of industries in various dimensions. For the various objects, such as people, processes, and data, involved in marketing activities, the Internet of Everything (IoE) as an evolved IoT is a possible future scenario. Some sectors pretend to be the first to implement this, and the more they rely on dynamic, unstable customer needs, the better a solution the IoE is for them. Therefore, this paper presents a clear vision of smart, sustainable marketing based on the IoE in one of the fast-moving consumer goods (FMCG) industries, the dairy industry. Key factors are identified to help readers understand this concept better. The expert interview makes it possible to draw a picture of the factors that have helped successfully implement the IoE in the dairy sector.
Chile (Capsicum spp.) as Food-Medicine Continuum in Multiethnic Mexico
Authors: Aguilar-Meléndez Araceli , Vásquez-Dávila Marco Antonio , Manzanero-Medina Gladys Isabel , Katz Esther
Published: October 2021

Mexico is the center of origin and diversification of domesticated chile (Capsicum annuum L.). Chile is conceived and employed as both food and medicine in Mexico. In this context, the objective of this paper is to describe and analyze the cultural role of chile as food and as medicine for the body and soul in different cultures of Mexico. To write it, we relied on our own fieldwork and literature review. Our findings include a) the first matrix of uses of chile across 67 indigenous and Afrodescendants cultures within Mexican territory and b) the proposal of a new model of diversified uses of chile. Traditional knowledge, uses and management of chile as food and medicine form a continuum (i.e., are not separated into distinct categories). The intermingled uses of Capsicum are diversified, deeply rooted and far-reaching into the past. Most of the knowledge, uses and practices are shared throughout Mexico. On the other hand, there is knowledge and practices that only occur in local or regional cultural contexts. In order to fulfill food, medicinal or spiritual functions, native communities use wild/cultivated chile.
Nutritional Metabolites of Red Pigmented Lettuce (Lactuca sativa) Germplasm and Correlations with Selected Phenotypic Characters
Authors: Assefa Awraris Derbie , Hur On-Sook , Hahn Bum-Soo , Kim Bichsaem , Ro Na-Young , Rhee Ju-Hee
Published: October 2021

Lettuce is an important dietary source of bioactive phytochemicals. Screening and identification of the health beneficial metabolites and evaluating the relationships with phenotypic characters can help consumers adjust their preferences for lettuce plant types. Thus, we explored the major health-beneficial individual metabolites and antioxidant potential of 113 red pigmented lettuce leaf samples. A UV–Vis spectrophotometer and UPLC-DAD-QTOF/MS (TQ/MS) instruments were used for the identification and quantification of metabolites and antioxidant activity accordingly. The metabolites were quantified against their corresponding external standards. The contents of metabolites varied significantly among lettuce samples. Cyanidin 3-O-(6″-O-malonyl)glucoside (4.7~5013.6 μg/g DW), 2,3-di-O-caffeoyltartaric acid (337.1~19,957.2 μg/g DW), and quercetin 3-O-(6″-O-malonyl)glucoside (45.4~31,121.0 μg/g DW) were the most dominant in red pigmented lettuce samples among anthocyanins, hydroxycinnamoyl derivatives, and flavonols, respectively. Lettuces with dark and very dark red pigmented leaves, circular leaf shape, a strong degree of leaf undulation, and highly dense leaf incisions were found to have high levels of flavonoids and hydroxycinnamoyl derivatives. Principal component analysis was used to investigate similarities and/or differences between samples, and the partial least square discriminant analysis classified them into known groups. The key variables that contributed highly were determined. Our report provides critical data on the bioactive constituents of red pigmented lettuce to breeders developing varieties with enhanced bioactive compounds and to nutraceutical companies developing nutrient dense foods and pharmaceutical formulations.
Role of Hydrocolloids in the Structure, Cooking, and Nutritional Properties of Fiber-Enriched, Fresh Egg Pasta Based on Tiger Nut Flour and Durum Wheat Semolina
Authors: Martín-Esparza Maria Eugenia , Raigón Maria Dolores , García-Martínez Maria Dolores , Albors Ana
Published: October 2021

The aim of this work concerns the manufacturing process of fresh egg tagliatelle labeled as a “source of fiber” based on tiger nut flour and wheat semolina. An attempt to improve the quality attributes and cooking properties of the obtained product was made by means of structuring agents. More specifically, a combination of three hydrocolloids (carboximethylcellulose, CMC; xanthan gum, XG; and locust bean gum, LBG) was tested. A Box–Behnken design with randomized response surface methodology was used to determine a suitable combination of these gums to achieve fewer cooking losses, higher water gain and swelling index values, and better texture characteristics before and after cooking. Positive effects on textural characteristics were observed when incorporating XG into the pasta formulation. Cooking and fiber loss also significantly diminished with the XG-CMC combination over 0.8%. No significant effect was found for the other evaluated parameters. A synergistic interaction between LBG and XG was only significant for the water absorption index. The cooked pasta was considered a source of fiber in all cases.
Atypical NF1 Microdeletions: Challenges and Opportunities for Genotype/Phenotype Correlations in Patients with Large NF1 Deletions
Authors: Kehrer-Sawatzki Hildegard , Wahlländer Ute , Cooper David N. , Mautner Victor-Felix
Published: October 2021

Patients with neurofibromatosis type 1 (NF1) and type 1 NF1 deletions often exhibit more severe clinical manifestations than patients with intragenic NF1 gene mutations, including facial dysmorphic features, overgrowth, severe global developmental delay, severe autistic symptoms and considerably reduced cognitive abilities, all of which are detectable from a very young age. Type 1 NF1 deletions encompass 1.4 Mb and are associated with the loss of 14 protein-coding genes, including NF1 and SUZ12. Atypical NF1 deletions, which do not encompass all 14 protein-coding genes located within the type 1 NF1 deletion region, have the potential to contribute to the delineation of the genotype/phenotype relationship in patients with NF1 microdeletions. Here, we review all atypical NF1 deletions reported to date as well as the clinical phenotype observed in the patients concerned. We compare these findings with those of a newly identified atypical NF1 deletion of 698 kb which, in addition to the NF1 gene, includes five genes located centromeric to NF1. The atypical NF1 deletion in this patient does not include the SUZ12 gene but does encompass CRLF3. Comparative analysis of such atypical NF1 deletions suggests that SUZ12 hemizygosity is likely to contribute significantly to the reduced cognitive abilities, severe global developmental delay and facial dysmorphisms observed in patients with type 1 NF1 deletions.
Association of PIP4K2A Polymorphisms with Alcohol Use Disorder
Authors: Fedorenko Olga Yu. , Mikhalitskaya Ekaterina V. , Toshchakova Valentina A. , Loonen Anton J. M. , Bokhan Nikolay A. , Ivanova Svetlana A.
Published: October 2021

Background: Alcohol use disorder (AUD) not only influences individuals and families but also has a lasting social impact on communities at the national level. Dopaminergic neurotransmission is involved in excessive alcohol consumption. Phosphatidylinositol-5-phosphate-4-kinase type 2 α (PIP4K2A) plays an important role in the regulation of ascending dopamine pathways. In this study; we determined possible associations between nine polymorphisms in PIP4K2A and AUD in Russian men. Methods: 279 Russian men with AUD were investigated. The control group consisted of 222 healthy men from the general Russian population. Genotyping of DNA samples for nine polymorphic variants of PIP4K2A was carried out by the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System with use of the TaqMan1 Validated SNP Genotyping Assay (Applied Biosystems; CIIIA). Results: Carriage of the PIP4K2A rs2230469*TT/T genotype/allele was a relative risk factor for developing AUD in men (p = 0.026 and p = 0.0084 accordingly). Moreover; men with AUD had a higher frequency of PIP4K2A rs746203*T allele (p = 0.023) compared to healthy men. Conclusions: For the first time; we demonstrated different PIP4K2A polymorphisms to be associated with AUD presumably due to dopamine system modulation resulting from regulation of the lateral habenula.
FA-nf: A Functional Annotation Pipeline for Proteins from Non-Model Organisms Implemented in Nextflow
Authors: Vlasova Anna , Hermoso Pulido Toni , Camara Francisco , Ponomarenko Julia , Guigó Roderic
Published: October 2021

Functional annotation allows adding biologically relevant information to predicted features in genomic sequences, and it is, therefore, an important procedure of any de novo genome sequencing project. It is also useful for proofreading and improving gene structural annotation. Here, we introduce FA-nf, a pipeline implemented in Nextflow, a versatile computational workflow management engine. The pipeline integrates different annotation approaches, such as NCBI BLAST+, DIAMOND, InterProScan, and KEGG. It starts from a protein sequence FASTA file and, optionally, a structural annotation file in GFF format, and produces several files, such as GO assignments, output summaries of the abovementioned programs and final annotation reports. The pipeline can be broken easily into smaller processes for the purpose of parallelization and easily deployed in a Linux computational environment, thanks to software containerization, thus helping to ensure full reproducibility.
Development and Effect of Virtual Reality Practice Program for Improving Practical Competency of Caregivers Specializing in Dementia
Authors: Kim Dooree
Published: October 2021

The number of dementia patients in Korea is increasing with the increase in the elderly population. Accordingly, the importance of the role of the caregivers, who are the main care worker other than the family, is increasing. Therefore, in this study, a virtual reality practice program was developed to enhance the practical competency of caregivers who take care of dementia patients, and the effects were analyzed. The caregiver said that among the mental behaviors of dementia patients, aggression and delusion were the most difficult. Based on this information, a practice program was developed by realizing a case of a male dementia patient who expressed refusal to bathing help as an aggressive behavior due to delusion in virtual reality, and the effect of the virtual reality practice program was analyzed for five caregivers. As a result, ‘interest in new teaching methods’, ‘improving concentration of practical education based on real cases’, and ‘increasing confidence in caring for dementia patients’ were found. As this study is a pilot test, it is necessary to repeat the study with more subjects in the future, and to develop virtual reality implementation cases for various mental and behavioral symptoms.
Borderline Personality Symptoms: What Not to Be Overlooked When Approaching Suicidal Ideation among University Students
Authors: Wongpakaran Nahathai , Oon-Arom Awirut , Karawekpanyawong Nuntaporn , Lohanan Trustsavin , Leesawat Thanakorn , Wongpakaran Tinakon
Published: October 2021

Suicidal ideation is a serious condition antecedent to suicidal attempts and is highly related not only to depression but also other psychosocial factors. This study aimed to examine the predictive effects of these potential factors for suicidal ideation among young adult university students. A cross-sectional survey was conducted on a sample of university students in Thailand. An online questionnaire employed the perceived stress scale-10 (PSS-10), the patient health questionnaire-8 (PHQ-8), the multidimensional scale of perceived social support (MSPSS), and a screening instrument for borderline personality disorder. An ordinal regression analysis was applied to determine the predictive effects of the independent variables. Of 336 students, the mean age was 20.26 ± 1.3 years, 80.4% of whom were female; 14.3% had suicidal ideation. The significant predictors of suicidal thoughts were perceived stress (AOR 1.11, 95% CI 1.01 to 1.22); depressive symptoms (AOR 1.16, 95% CI 1.05 to 1.22); borderline personality symptoms (AOR 1.19, 95% CI 1.01 to 1.40); and perceived social support (AOR 0.97, 95% CI 0.94 to 1.00). Not only did depressive symptoms contribute to suicidal ideation but they also constituted important variables. Therefore, they should be included in intervention plans to prevent suicidality among university students.
Physical, Anatomical, and Biochemical Composition of Skins Cell Walls from Two Grapevine Cultivars (Vitis vinifera) of Champagne Region Related to Their Susceptibility to Botrytis cinerea during Ripening
Authors: André Marie , Lacampagne Soizic , Barsacq Audrey , Gontier Etienne , Petrel Melina , Mercier Laurence , Courot Diane , Gény-Denis Laurence
Published: October 2021

This work investigated the structural, biochemical, and molecular characteristics of grape skin cell wall during ripening, related to susceptibility to Botrytis cinerea. The comparative study between the two main grape cultivars in Champagne region, Pinot noir and Chardonnay, quantified: (1) the maturity and physical profile of grape skin; (2) the morphological characteristics; (3) soluble pectic polysaccharides located in grape skin cell walls; and (4) the gene expression of the two main degrading enzymes (VvPME1 and VvPG1) and PME activity. During the maturation period, the grape skins of the two cultivars appear different in their structure and composition. Chardonnay is characterized by higher relative humidity (RH) and level of VvPG1 expression, lower disease incidence and penetrometry values, and thicker cell walls than Pinot noir skins. Thus, the cell wall composition is sufficiently different between grape varieties from the same area to allow their discrimination and could be used to better manage the harvest date.
Reply to Vale et al. Comment on “Zhong et al. Sugar-Sweetened and Diet Beverage Consumption in Philadelphia One Year after the Beverage Tax. Int. J. Environ. Res. Public Health 2020, 17, 1336”
Authors: Zhong Yichen , Auchincloss Amy H. , Lee Brian K. , McKenna Ryan M. , Langellier Brent A.
Published: October 2021

Thank you for the opportunity to respond to the recent letter to the editor regarding our paper “Sugar-Sweetened and Diet Beverage Consumption in Philadelphia One Year after the Beverage Tax” [...]
Psychotherapeutic Interventions to Improve Psychological Adjustment in Type 1 Diabetes: A Systematic Review
Authors: Resurrección Davinia M. , Navas-Campaña Desirée , Gutiérrez-Colosía Mencía R. , Ibáñez-Alfonso Joaquín A. , Ruiz-Aranda Desireé
Published: October 2021

Background: International clinical practice guidelines highlight the importance of improving the psychological and mental health care of patients with Type 1 diabetes mellitus (T1DM). Psychological interventions can promote adherence to the demands of diabetes self-care, promoting high quality of life and wellbeing. Methods: A systematic review was carried out to determine whether psychological treatments with a specific focus on emotional management have an impact on glycemic control and variables related to psychological adjustment. Comprehensive literature searches of PubMed Medline, Psycinfo, Cochrane Database, Web of Science, and Open Grey Repository databases were conducted, from inception to November 2019 and were last updated in December 2020. Finally, eight articles met inclusion criteria. Results: Results showed that the management of emotions was effective in improving the psychological adjustment of patients with T1DM when carried out by psychologists. However, the evidence regarding the improvement of glycemic control was not entirely clear. When comparing adolescent and adult populations, findings yielded slightly better results in adolescents. Conclusions: More rigorous studies are needed to establish what emotional interventions might increase glycemic control in this population.
The Relationships between Perceived Design Intensity, Preference, Restorativeness and Eye Movements in Designed Urban Green Space
Authors: Wu Yu , Zhuo Zhixiong , Liu Qunyue , Yu Kunyong , Huang Qitang , Liu Jian
Published: October 2021

Recent research has demonstrated that landscape design intensity impacts individuals’ landscape preferences, which may influence their eye movement. Due to the close relationship between restorativeness and landscape preference, we further explore the relationships between design intensity, preference, restorativeness and eye movements. Specifically, using manipulated images as stimuli for 200 students as participants, the effect of urban green space (UGS) design intensity on landscapes’ preference, restorativeness, and eye movement was examined. The results demonstrate that landscape design intensity could contribute to preference and restorativeness and that there is a significant positive relationship between design intensity and eye-tracking metrics, including dwell time percent, fixation percent, fixation count, and visited ranking. Additionally, preference was positively related to restorativeness, dwell time percent, fixation percent, and fixation count, and there is a significant positive relationship between restorativeness and fixation percent. We obtained the most feasible regression equations between design intensity and preference, restorativeness, and eye movement. These results provide a set of guidelines for improving UGS design to achieve its greatest restorative potential and shed new light on the use of eye-tracking technology in landscape perception studies.
Factors Influencing Retirement Decisions among Blue-Collar Workers in a Global Manufacturing Company—Implications for Age Management from A System Perspective
Authors: Jaldestad Ellen , Eriksson Andrea , Blom Philip , Östlund Britt
Published: October 2021

The maintenance of older workers and determining the appropriate age for retirement are growing issues related to the fact that fewer people, still active in working life, have to provide for more non-working people due to increased life expectancy. As a result, retirement age has started to rise in many countries, and employers need to find ways to maintain an older and healthy work force, not least to avoid the loss of important experience. The aim of the current study was to increase the knowledge of factors influencing the retirement decisions among blue-collar workers in different national settings. A survey and semi-structured interviews were conducted with a sample of 100 blue-collar workers in Sweden, the Netherlands, and France, aged 55 years and older, within a global manufacturing company. Based on the results, implications for companies’ age management strategies were discussed from a system perspective. Factors contributing to both retirement and to a prolonged work life were found on individual, organisational, and societal levels. This indicates the importance of a system perspective when planning for age management interventions.
“… and after That Came Me”. Subjective Constructions of Social Hierarchy in Physical Education Classes among Youth with Visual Impairments in Germany
Authors: Giese Martin , Ruin Sebastian , Baumgärtner Jana , Haegele Justin A.
Published: October 2021

The aim of this study was to reconstruct subjective constructions of experiences in PE and feelings of being valued within PE classes in Germany by students with visual impairment (VI). Two female and two male students (average age: 19.25 years) participated in the study from the upper level. For the reconstruction of experiences of feeling valued, episodic interviews with a semi-structured interview guide were used. The data analysis was conducted with MAXQDA 2020 based on content-related structuring of qualitative text analysis with deductive–inductive category formation. To structure the analysis, the main category, feelings of being valued, was defined by two poles (positive feelings of being valued as opposed to bullying). As a main finding, respondents primarily reported negative feelings and experiences characterized by instances of bullying, discrimination, and physical and social isolation, perpetuated by both their peers and teachers. In search of a deeper understanding, we identified social hierarchy as an underlying structure determining the students’ perceived positioning within the social context and thus directing their feelings of being (de-)valued. It became evident that it is not the setting per se that determined social hierarchy, but that it is more about the concrete manifestation of social hierarchy.
Positive Behavior Management: Assessment of Rugby Referees in Children Sport
Authors: Płoszaj Katarzyna , Firek Wiesław , Gąsior Paweł , Malchrowicz-Mośko Ewa
Published: October 2021

During children’s sports competitions, the referees play a special role. The referees are expected to be able to identify behavioral problems (of players, coaches, and fans), applying specific techniques to prevent negative behavior of players. Adapting these actions to the specifics of the group or individuals is crucial in providing a safe educational environment that promotes child development. The main objective of this research was to assess the quality of referees’ interactions with players in terms of positive behavior management and proficiency during rugby matches of children aged 6–12 years. Twenty-three rugby referees officiating matches organized by the Polish Rugby Union in Poland participated in the study. The research used the Referee–Players’ Interaction Assessment Scoring System tool. Additionally, referee–player interactions were recorded with a GoPro 8 camera along with audio from a wireless intercom. The significance of differences between the ratings for each indicator was tested by chi-squared test, while a non-parametric Wilcoxon signed-rank test was used to compare the mean ratings of positive behavior management and proficiency. The Mann–Whitney U-test was used to compare differences between assessments of experienced and inexperienced referees. The observations showed that referees were rated significantly higher in the proficiency dimension than in positive behavior management dimension. Nevertheless, both ratings represent an average level of quality of interactions with the players. The referee’s experience did not determine the quality of his or her interactions with the players in the specific dimensions. The following conclusion was drawn from the research: referees should be trained in the methods and techniques for managing player behavior and should act to prevent the occurrence of negative behavior, by presenting clear and understandable expectations to players before the match and using preventive measures.