bluuebunny/crossref_metadata_2025 huggingface dataset:
The Association between Salt Taste Perception, Mediterranean Diet and Metabolic Syndrome: A Cross-Sectional Study
Authors: Veček Nikolina Nika , Mucalo Lana , Dragun Ružica , Miličević Tanja , Pribisalić Ajka , Patarčić Inga , Hayward Caroline , Polašek Ozren , Kolčić Ivana
Published: April 2020

Metabolic syndrome (MetS) is a widespread disorder and an important public health challenge. The purpose of this study was to identify the association between salt taste perception, Mediterranean diet and MetS. This cross-sectional study included 2798 subjects from the general population of Dalmatia, Croatia. MetS was determined using the Joint Interim Statement definition, and Mediterranean diet compliance was estimated using Mediterranean Diet Serving Score. Salt taste perception was assessed by threshold and suprathreshold testing (intensity and hedonic perception). Logistic regression was used in the analysis, adjusting for important confounding factors. As many as 44% of subjects had MetS, with elevated waist circumference as the most common component (77%). Higher salt taste sensitivity (lower threshold) was associated with several positive outcomes: lower odds of MetS (OR = 0.69; 95% CI 0.52–0.92), lower odds for elevated waist circumference (0.47; 0.27–0.82), elevated fasting glucose or diabetes (0.65; 0.45–0.94), and reduced HDL cholesterol (0.59; 0.42–0.84), compared to the higher threshold group. Subjects with lower salt taste threshold were more likely to consume more fruit, and less likely to adhere to olive oil and white meat guidelines, but without a difference in the overall Mediterranean diet compliance. Salt taste intensity perception was not associated with any of the investigated outcomes, while salty solution liking was associated with MetS (OR = 1.85, CI 95% 1.02–3.35). This study identified an association between salt taste perception and MetS and gave a new insight into taste perception, nutrition, and possible health outcomes.
Dietary Intervention in Pregnant Women with Gestational Diabetes; Protocol for the DiGest Randomised Controlled Trial
Authors: Kusinski Laura C. , Murphy Helen R. , De Lucia Rolfe Emanuella , Rennie Kirsten L. , Oude Griep Linda M. , Hughes Deborah , Taylor Roy , Meek Claire L.
Published: April 2020

Gestational diabetes mellitus (GDM) annually affects 35,000 pregnancies in the United Kingdom, causing suboptimal health outcomes to the mother and child. Obesity and excessive gestational weight gain are risk factors for GDM. The Institute of Medicine recommends weight targets for women that are overweight and obese, however, there are no clear guidelines for women with GDM. Observational data suggest that modest weight loss (0.6–2 kg) after 28 weeks may reduce risk of caesarean section, large-for-gestational-age (LGA), and maternal postnatal glycaemia. This protocol for a multicentre randomised double-blind controlled trial aims to identify if a fully controlled reduced energy diet in GDM pregnancy improves infant birthweight and reduces maternal weight gain (primary outcomes). A total of 500 women with GDM (National Institute of Health and Care Excellence (NICE) 2015 criteria) and body mass index (BMI) ≥25 kg/m2 will be randomised to receive a standard (2000 kcal/day) or reduced energy (1200 kcal/day) diet box containing all meals and snacks from 28 weeks to delivery. Women and caregivers will be blinded to the allocations. Food diaries, continuous glucose monitoring, and anthropometry will measure dietary compliance, glucose levels, and weight changes. Women will receive standard antenatal GDM management (insulin/metformin) according to NICE guidelines. The secondary endpoints include caesarean section rates, LGA, and maternal postnatal glucose concentrations.
Efficacy of Vitamins on Cognitive Function of Non-Demented People: A Systematic Review and Meta-Analysis
Authors: Suh Seung Wan , Kim Hye Sung , Han Ji Hyun , Bae Jong Bin , Oh Dae Jong , Han Ji Won , Kim Ki Woong
Published: April 2020

Previous evidence has suggested that vitamins might be beneficial for cognition. This systematic review aimed to investigate the efficacy of B vitamins, antioxidant vitamins, and vitamin D on the cognitive function of non-demented middle-aged or older people. Randomized or quasi-randomized controlled trials of individuals aged 40 years or older were included. PubMed/MEDLINE, EMBASE, CINAHL, PsycINFO, Cochrane Library databases, and other grey literature sources were searched up to November 2019. Their methodological quality was evaluated using the Cochrane Risk of Bias tool. Twenty-three studies on B vitamins (n = 22–1053; comprising folate, B6, and B12), nine on antioxidant vitamins (n = 185–20,469), and six on vitamin D (n = 55–4122) were included. Taking B vitamins for over 3 months was beneficial for global cognition (standardized mean difference (SMD) −0.18, 95% CI −0.30 to −0.06) and episodic memory (SMD −0.09, 95% CI −0.15 to −0.04). However, antioxidant vitamins (SMD −0.02, 95% CI −0.08 to 0.03) and vitamin D (SMD −0.06, 95% CI −0.36 to 0.23) were not. Antioxidant vitamins were beneficial for global cognition in sensitivity analyses using final measurement data as mean difference estimates (SMD, −0.04, 95% CI −0.08 to −0.01). Taking B vitamins and possibly antioxidant vitamins may be beneficial for the cognitive function of non-demented people.
Maternal Allergy and the Presence of Nonhuman Proteinaceous Molecules in Human Milk
Authors: Dekker Pieter M. , Boeren Sjef , Wijga Alet H. , Koppelman Gerard H. , Vervoort Jacques J. M. , Hettinga Kasper A.
Published: April 2020

Human milk contains proteins and/or protein fragments that originate from nonhuman organisms. These proteinaceous molecules, of which the secretion might be related to the mother’s allergy status, could be involved in the development of the immune system of the infant. This may lead, for example, to sensitization or the induction of allergen-specific tolerance. The aim of this study was to investigate the relation between maternal allergy and the levels of nonhuman proteinaceous molecules in their milk. In this study, we analysed trypsin-digested human milk serum proteins of 10 allergic mothers and 10 nonallergic mothers. A search was carried out to identify peptide sequences originating from bovine or other allergenic proteins. Several methods were applied to confirm the identification of these sequences, and the differences between both groups were investigated. Out of the 78 identified nonhuman peptide sequences, 62 sequences matched Bos taurus proteins. Eight peptide sequences of bovine β -lactoglobulin had significantly higher levels in milk from allergic mothers than in milk from nonallergic mothers. Dietary bovine β -lactoglobulin may be absorbed through the intestinal barrier and secreted into human milk. This seems to be significantly higher in allergic mothers and might have consequences for the development of the immune system of their breastfed infant.
Lysophosphatidylcholine Containing Anisic Acid Is Able to Stimulate Insulin Secretion Targeting G Protein Coupled Receptors
Authors: Drzazga Anna , Okulus Marta , Rychlicka Magdalena , Biegała Łukasz , Gliszczyńska Anna , Gendaszewska-Darmach Edyta
Published: April 2020

Diabetes mellitus is a worldwide health problem with high rates of mortality and morbidity. Management of diabetes mellitus by dietary components is achievable especially at the initial stage of the disease. Several studies confirmed the antidiabetic activities of simple phenolic acids and lysophosphatidylcholine (LPC). The main goal of this study was to identify new potential insulin secretion modulators obtained by combining the structures of two natural compounds, namely O-methyl derivatives of phenolic acids and phospholipids. LPC and phosphatidylcholine bearing methoxylated aromatic carboxylic acids were tested as potential agents able to improve glucose-stimulated insulin secretion (GSIS) and intracellular calcium mobilization in MIN6 β pancreatic cell line. Our results show that LPC with covalently bonded molecule of p-anisic acid at the sn-1 position was able to induce GSIS and intracellular calcium flux. Notably, 1-anisoyl-2-hydroxy-sn-glycero-3-phosphocholine did not affect the viability of MIN6 cells, suggesting its potential safe use. Furthermore, we have shown that three G protein coupled receptors, namely GPR40, GPR55, and GPR119, are targeted by this LPC derivative.
Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells
Authors: Foisy-Sauvé Mathilde , Ahmarani Lena , Delvin Edgard , Sané Alain T. , Spahis Schohraya , Levy Emile
Published: April 2020

Background. Metabolic Syndrome (MetS), a major worldwide concern for the public health system, refers to a cluster of key metabolic components, and represents a risk factor for diabetes and cardiovascular diseases. As oxidative stress (OxS) and inflammation are the major triggers of insulin sensitivity (IS), a cardinal MetS feature, the principal aim of the present work is to determine whether glycomacropeptide (GMP), a milk-derived bioactive peptide, exerts beneficial effects on their expression. Methods. Fully differentiated intestinal Caco-2/15 cells are used to evaluate the preventive action of 2 mg/mL GMP against OxS and inflammation induced by the mixture iron-ascorbate (Fe/Asc) (200 μM:2 mM). The potency of GMP of decreasing the production of lipoproteins, including chylomicrons (CM), very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) is also assessed. Results. The administration of GMP significantly reduces malondialdehyde, a biomarker of lipid peroxidation, and raises superoxide dismutase 2 and glutathione peroxidase via the induction of the nuclear factor erythroid 2–related factor 2, a transcription factor, which orchestrates cellular antioxidant defenses. Similarly, GMP markedly lowers the inflammatory agents tumor necrosis factor-α and cyclooxygenase-2 via abrogation of the nuclear transcription factor-kB. Moreover, GMP-treated cells show a down-regulation of Fe/Asc-induced mitogen activated protein kinase pathway, suggesting greater IS. Finally, GMP decreases the production of CM, VLDL, and LDL. Conclusions. Our results highlight the effectiveness of GMP in attenuating OxS, inflammation and lipoprotein biogenesis, as well as improving IS, the key components of MetS. Further investigation is needed to elucidate the mechanisms mediating the preventive action of GMP.
Acute Ingestion of a Novel Nitrate-Rich Dietary Supplement Significantly Increases Plasma Nitrate/Nitrite in Physically Active Men and Women
Authors: Bloomer Richard J. , Butawan Matthew , Pigg Brandon , Martin Keith R.
Published: April 2020

Background: Dietary supplements purported to increase circulating nitric oxide are very popular among consumers. We determined the acute impact of two novel dietary supplements on plasma nitrate/nitrite (NOx) and nitrite alone. Methods: 20 men and women (age: 24 ± 5 years) ingested two different nitrate-rich supplements (Resync Recovery Blend at 7.5 g and 15 g; Resync Collagen Blend at 21 g), or placebo, on four different days. Fasting blood samples were obtained before and 75 min following ingestion and analyzed for NOx and nitrite. Results: Nitrite was not differently impacted by treatment (p > 0.05). The NOx response for men and women was very similar, with no sex interactions noted (p > 0.05). Condition (p < 0.0001), time (p < 0.0001), and condition x time (p < 0.0001) effects were noted for NOx. Values increased from baseline to post-ingestion for the Resync Recovery Blend at 7.5 g (11 ± 9 to 101 ± 48 µM) and at 15 g (9 ± 5 to 176 ± 91 µM), as well as for the Resync Collagen Blend (9 ± 9 to 46 ± 21 µM), while values for placebo remained stable (9 ± 7 to 8 ± 5 µM). Conclusion: While nitrite alone was not impacted by treatment, both Resync products result in an increase in plasma NOx, with the increase proportionate to the quantity of “nitric oxide blend” ingredients contained within each product. Future studies are needed to determine the physiological implications of the increased NOx, as pertaining to exercise performance and recovery, in addition to other aspects of human health.
Acute Effect of the Timing of Resistance Exercise and Nutrient Intake on Muscle Protein Breakdown
Authors: Kume Wataru , Yasuda Jun , Hashimoto Takeshi
Published: April 2020

Background: Combining resistance exercise (RE) with nutrient intake stimulates muscle protein net balance. However, it is still unclear whether the optimal timing of nutrient intake is before or after RE, especially on muscle protein breakdown (MPB) for an augmented muscle anabolic response. The aim of this study was to investigate the effect of a substantial mixed meal (i.e., nutrient- and protein-dense whole foods) before or after RE, compared with RE without a meal on the acute response of MPB in a crossover-design study. Methods: Eight healthy young men performed three trials: (1) meal intake before RE (Pre), (2) meal intake after RE (Post), and (3) RE without meal intake (No). Plasma insulin and 3-methylhistidine (3-MH), an MPB marker, were measured. Results: Time course change in plasma insulin level after RE was significantly higher in the Post condition than in the Pre and No conditions. The area under the curve of 3-MH concentration was significantly lower in the Post condition than in the Pre and No conditions. Conclusions: These results suggest that a substantial mixed meal immediately after RE may effectively suppress MPB in the morning.
Effect of a 12-Week Almond-Enriched Diet on Biomarkers of Cognitive Performance, Mood, and Cardiometabolic Health in Older Overweight Adults
Authors: Coates Alison Mary , Morgillo Samantha , Yandell Catherine , Scholey Andrew , Buckley Jonathan David , Dyer Kathryn Ann , Hill Alison Marie
Published: April 2020

Long term nut consumption is associated with reduced risk of coronary heart disease and better cognitive function. This study examined supplementing habitual diets with almonds or carbohydrate-rich snack foods (providing 15% energy) on biomarkers of cardiovascular and metabolic health, mood and cognitive performance. Participants (overweight/obese, 50–80 years) were randomised to an almond-enriched diet (AED) or isocaloric nut-free diet (NFD) for 12 weeks. Body weight, blood lipids, glucose, insulin, blood pressure (BP), arterial stiffness, cell adhesions molecules, C reactive protein (CRP), mood, and cognitive performance (working memory primary outcome), dietary profiles and energy intake/expenditure were measured at baseline and Week 12 in 128 participants (n = 63 AED, n = 65 NFD). Compared with NFD, AED was associated with altered macro and micronutrient profiles, but no differences in energy intake or expenditure. The AED significantly reduced triglycerides and SBP but there were no other changes in cardiometabolic biomarkers, mood, or cognitive performance. The inclusion of almonds in the diet improves aspects of cardiometabolic health without affecting cognitive performance or mood in overweight/obese adults.
Reply to “Limitations of the Digestible Indispensable Amino Acid Score (DIAAS) and Choice of Statistical Reporting. Comment on “A Comparison of Dietary Protein Digestibility, Based on DIAAS Scoring, in Vegetarian and Non-Vegetarian Athletes.” Nutrients 2019, 11, 3106”
Authors: Johnston Carol S. , Lynch Heidi , Wharton Christopher
Published: April 2020

We appreciate the critique of our paper (Ciuris et al [...]
Association of Basal Metabolic Rate and Nutrients Oxidation with Cardiometabolic Risk Factors and Insulin Sensitivity in Sedentary Middle-Aged Adults
Authors: Amaro-Gahete Francisco , Jurado-Fasoli Lucas , Ruiz Jonatan , Castillo Manuel
Published: April 2020

This cross-sectional study aimed to examine the association of basal metabolic rate (BMR) and basal fat and carbohydrate oxidation (BFox and BCHox, respectively) with cardiometabolic risk factors and insulin sensitivity in sedentary middle-aged adults. A total of 71 healthy sedentary adults (37 women) aged 40–65 years participated in the current study. Data were collected during the baseline assessments of the FIT-AGEING randomized controlled trial. BMR was measured via indirect calorimetry, and BFox and BCHox estimated by stoichiometric equations. Blood pressure, glucose, insulin, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides plasma levels were selected as cardiometabolic risk factors and assessed following standard procedures. We observed positive associations of BMR with plasma insulin and the homeostatic model assessment of insulin resistance index (HOMA; all p < 0.05) which were attenuated or disappeared after controlling by sex, age, and/or lean mass. There were positive associations between BFox and the quantitative insulin sensitivity check index (QUICKI; p < 0.015), while negative associations were noted between BFox and plasma insulin and HOMA (p < 0.015). There was a significant negative association between BCHox with QUICKI (p < 0.01), whereas significant positive relationships were obtained when BCHox was associated with plasma insulin and HOMA (p < 0.01). These associations persisted in almost all cases when controlling by sex, age and/or lean mass. No further relationships were found when BMR, BFox, and BCHox were associated with other cardiometabolic risk factors. In conclusion, our study findings support that greater BFox and lower BCHox are related to improved insulin sensitivity, whereas BMR seems to be not associated with neither cardiometabolic risk nor insulin sensitivity in sedentary middle-aged adults. Further intervention studies are necessary to well-understand the physiological mechanism implied in this relationship.
Genetic Diversity and Recombination in the Plant Pathogen Sclerotinia sclerotiorum Detected in Sri Lanka
Authors: Mahalingam Thirega , Chen Weidong , Rajapakse Chandima Shashikala , Somachandra Kandangamuwa Pathirannahalage , Attanayake Renuka Nilmini
Published: April 2020

Sclerotinia sclerotiorum is an important fungal pathogen on many economically important crops including cabbage worldwide. Even though population structure and genetic diversity of S. sclerotiorum is well studied in temperate climatic conditions, only a few studies have been conducted in tropical countries. It is also not clear whether the populations are clonal or recombining in the tropics. In filling this information gap, 47 isolates of S. sclerotiorum were collected from commercial cabbage (Brassica oleracea L.) fields in Nuwara Eliya district of Sri Lanka, where the disease has been previously reported. All the isolates were subjected to genetic diversity study using mycelial compatibility grouping and microsatellite markers. Fourteen mycelial compatibility groups (MCGs) and 23 multilocus haplotypes (MLHs) were recorded. Mean expected heterozygosity of the population was 0.56. MLHs were weakly correlated with MCGs. Population genetic structure analysis and principal coordinates identified three genetic clusters. Genetic recombination was inferred within each genetic cluster when isolates were subjected to clone correction. There was evidence of multiple infections on single plant as detected by the presence of more than one MCG on each cabbage plant. However, multiple infections did not increase the disease severity in detached leaf assay. We found high genetic diversity and recombination of S. sclerotiorum population in a tropical country, Sri Lanka. Importance of detecting genetic structure when inferring recombination was also highlighted.
Origin, Potential Therapeutic Targets and Treatment for Coronavirus Disease (COVID-19)
Authors: Nadeem Muhammad Shahid , Zamzami Mazin A. , Choudhry Hani , Murtaza Bibi Nazia , Kazmi Imran , Ahmad Habib , Shakoori Abdul Rauf
Published: April 2020

The ongoing episode of coronavirus disease 19 (COVID-19) has imposed a serious threat to global health and the world economy. The disease has rapidly acquired a pandemic status affecting almost all populated areas of the planet. The causative agent of COVID-19 is a novel coronavirus known as SARS-CoV-2. The virus has an approximate 30 kb single-stranded positive-sense RNA genome, which is 74.5% to 99% identical to that of SARS-CoV, CoV-pangolin, and the coronavirus the from horseshoe bat. According to available information, SARS-CoV-2 is inferred to be a recombinant virus that originated from bats and was transmitted to humans, possibly using the pangolin as the intermediate host. The interaction of the SARS-CoV-2 spike protein with the human ACE2 (angiotensin-converting enzyme 2) receptor, and its subsequent cleavage by serine protease and fusion, are the main events in the pathophysiology. The serine protease inhibitors, spike protein-based vaccines, or ACE2 blockers may have therapeutic potential in the near future. At present, no vaccine is available against COVID-19. The disease is being treated with antiviral, antimalarial, anti-inflammatory, herbal medicines, and active plasma antibodies. In this context, the present review article provides a cumulative account of the recent information regarding the viral characteristics, potential therapeutic targets, treatment options, and prospective research questions.
Effects of Mycoplasmas on the Host Cell Signaling Pathways
Authors: Borchsenius Sergei N. , Vishnyakov Innokentii E. , Chernova Olga A. , Chernov Vladislav M. , Barlev Nikolai A.
Published: April 2020

Mycoplasmas are the smallest free-living organisms. Reduced sizes of their genomes put constraints on the ability of these bacteria to live autonomously and make them highly dependent on the nutrients produced by host cells. Importantly, at the organism level, mycoplasmal infections may cause pathological changes to the host, including cancer and severe immunological reactions. At the molecular level, mycoplasmas often activate the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) inflammatory response and concomitantly inhibit the p53-mediated response, which normally triggers the cell cycle and apoptosis. Thus, mycoplasmal infections may be considered as cancer-associated factors. At the same time, mycoplasmas through their membrane lipoproteins (LAMPs) along with lipoprotein derivatives (lipopeptide MALP-2, macrophage-activating lipopeptide-2) are able to modulate anti-inflammatory responses via nuclear translocation and activation of Nrf2 (the nuclear factor-E2-related anti-inflammatory transcription factor 2). Thus, interactions between mycoplasmas and host cells are multifaceted and depend on the cellular context. In this review, we summarize the current information on the role of mycoplasmas in affecting the host’s intracellular signaling mediated by the interactions between transcriptional factors p53, Nrf2, and NF-κB. A better understanding of the mechanisms underlying pathologic processes associated with reprogramming eukaryotic cells that arise during the mycoplasma-host cell interaction should facilitate the development of new therapeutic approaches to treat oncogenic and inflammatory processes.
Importance of Mesoporous Silica Particle Size in the Stabilization of Amorphous Pharmaceuticals—The Case of Simvastatin
Authors: Knapik-Kowalczuk Justyna , Kramarczyk Daniel , Chmiel Krzysztof , Romanova Jana , Kawakami Kohsaku , Paluch Marian
Published: April 2020

In this paper, the role of mesoporous silica (MS) particle size in the stabilization of amorphous simvastatin (SVT) is revealed. For inhibiting recrystallization of the supercooled drug, the two MS materials (Syloid® XDP 3050 and Syloid® 244 FP) were employed. The crystallization tendency of SVT alone and in mixture with the MS materials was investigated by Differential Scanning Calorimetry (DSC) and Broadband Dielectric Spectroscopy (BDS). Neither confinement of the SVT molecules inside the MS pores nor molecular interactions between functional groups of the SVT molecules and the surface of the stabilizing excipient could explain the observed stabilization effect. The stabilization effect might be correlated with diffusion length of the SVT molecules in the MS materials that depended on the particle size. Moreover, MS materials possessing different particle sizes could offer free spaces with different sizes, which might influence crystal growth of SVT. All of these factors must be considered when mesoporous materials are used for stabilizing pharmaceutical glasses.
Unraveling the Phylogenomic Relationships of the Most Diverse African Palm Genus Raphia (Calamoideae, Arecaceae)
Authors: Helmstetter Andrew J. , Kamga Suzanne Mogue , Bethune Kevin , Lautenschläger Thea , Zizka Alexander , Bacon Christine D. , Wieringa Jan J. , Stauffer Fred , Antonelli Alexandre , Sonké Bonaventure , Couvreur Thomas L. P.
Published: April 2020

Palms are conspicuous floristic elements across the tropics. In continental Africa, even though there are less than 70 documented species, they are omnipresent across the tropical landscape. The genus Raphia has 20 accepted species in Africa and one species endemic to the Neotropics. It is the most economically important genus of African palms with most of its species producing food and construction material. Raphia is divided into five sections based on inflorescence morphology. Nevertheless, the taxonomy of Raphia is problematic with no intra-generic phylogenetic study available. We present a phylogenetic study of the genus using a targeted exon capture approach sequencing of 56 individuals representing 18 out of the 21 species. Our results recovered five well supported clades within the genus. Three sections correspond to those based on inflorescence morphology. R. regalis is strongly supported as sister to all other Raphia species and is placed into a newly described section: Erectae. Overall, morphological based identifications agreed well with our phylogenetic analyses, with 12 species recovered as monophyletic based on our sampling. Species delimitation analyses recovered 17 or 23 species depending on the confidence level used. Species delimitation is especially problematic in the Raphiate and Temulentae sections. In addition, our clustering analysis using SNP data suggested that individual clusters matched geographic distribution. The Neotropical species R. taedigera is supported as a distinct species, rejecting the hypothesis of a recent introduction into South America. Our analyses support the hypothesis that the Raphia individuals from Madagascar are potentially a distinct species different from the widely distributed R. farinifera. In conclusion, our results support the infra generic classification of Raphia based on inflorescence morphology, which is shown to be phylogenetically useful. Classification and species delimitation within sections remains problematic even with our phylogenomic approach. Certain widely distributed species could potentially contain cryptic species. More in-depth studies should be undertaken using morphometrics, increased sampling, and more variable markers. Our study provides a robust phylogenomic framework that enables further investigation on the biogeographic history, morphological evolution, and other eco-evolutionary aspects of this charismatic, socially, and economically important palm genus.
UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels
Authors: Diez-Ahedo Ruth , Mendibil Xabier , Márquez-Posadas Mari Carmen , Quintana Iban , González Francisco , Rodríguez Francisco Javier , Zilic Leyla , Sherborne Colin , Glen Adam , Taylor Caroline S. , Claeyssens Frederik , Haycock John W. , Schaafsma Wandert , González Eva , Castro Begoña , Merino Santos
Published: April 2020

Peripheral nerves are basic communication structures guiding motor and sensory information from the central nervous system to receptor units. Severed peripheral nerve injuries represent a large clinical problem with relevant challenges to successful synthetic nerve repair scaffolds as substitutes to autologous nerve grafting. Numerous studies reported the use of hollow tubes made of synthetic polymers sutured between severed nerve stumps to promote nerve regeneration while providing protection for external factors, such as scar tissue formation and inflammation. Few approaches have described the potential use of a lumen structure comprised of microchannels or microfibers to provide axon growth avoiding misdirection and fostering proper healing. Here, we report the use of a 3D porous microchannel-based structure made of a photocurable methacrylated polycaprolactone, whose mechanical properties are comparable to native nerves. The neuro-regenerative properties of the polymer were assessed in vitro, prior to the implantation of the 3D porous structure, in a 6-mm rat sciatic nerve gap injury. The manufactured implants were biocompatible and able to be resorbed by the host’s body at a suitable rate, allowing the complete healing of the nerve. The innovative design of the highly porous structure with the axon guiding microchannels, along with the observation of myelinated axons and Schwann cells in the in vivo tests, led to a significant progress towards the standardized use of synthetic 3D multichannel-based structures in peripheral nerve surgery.
Straightforward Synthetic Protocol to Bio-Based Unsaturated Poly(ester amide)s from Itaconic Acid with Thixotropic Behavior
Authors: Papadopoulos Lazaros , Kluge Marcel , Bikiaris Dimitrios N. , Robert Tobias
Published: April 2020

In the field of polymer chemistry, tremendous efforts have been made over the last decade to replace petrochemical monomers with building blocks from renewable resources. In this respect, itaconic acid has been used as an alternative to acrylic acid or maleic acid in unsaturated polyesters for thermal or UV-curing applications. However, examples of poly(ester amide)s from itaconic acid are scarce. Under standard polycondensation reactions, the presence of free amines leads to aza-Michael addition reactions at the α,β-unsaturated double bond of the itaconic acid and isomerization reactions to mesaconic acid. Both reactions make the resulting materials useless as UV-curing polymer resins. To avoid these undesired side reactions, we herein report the use of preformed, well-defined diols containing internal amide bonds. The resulting unsaturated poly(ester amide) resins were analyzed before and after UV-induced crosslinking. Viscosity measurements revealed a strong thixotropic behavior induced by the amide groups, which is usually not detected in structurally similar polyester resins.
Effect of Ionic Radius in Metal Nitrate on Pore Generation of Cellulose Acetate in Polymer Nanocomposite
Authors: Lee Woong Gi , Cho Younghyun , Kang Sang Wook
Published: April 2020

To prepare a porous cellulose acetate (CA) for application as a battery separator, Cd(NO3)2·4H2O was utilized with water-pressure as an external physical force. When the CA was complexed with Cd(NO3)2·4H2O and exposed to external water-pressure, the water-flux through the CA was observed, indicating the generation of pores in the polymer. Furthermore, as the hydraulic pressure increased, the water-flux increased proportionally, indicating the possibility of control for the porosity and pore size. Surprisingly, the value above 250 LMH (L/m2h) observed at the ratio of 1:0.35 (mole ratio of CA: Cd(NO3)2·4H2O) was of higher flux than those of CA/other metal nitrate salts (Ni(NO3)2 and Mg(NO3)2) complexes. The higher value indicated that the larger and abundant pores were generated in the cellulose acetate at the same water-pressure. Thus, it could be thought that the Cd(NO3)2·4H2O salt played a role as a stronger plasticizer than the other metal nitrate salts such as Ni(NO3)2 and Mg(NO3)2. These results were attributable to the fact that the atomic radius and ionic radius of the Cd were largest among the three elements, resulting in the relatively larger Cd of the Cd(NO3)2 that could easily be dissociated into cations and NO3− ions. As a result, the free NO3− ions could be readily hydrated with water molecules, causing the plasticization effect on the chains of cellulose acetate. The coordinative interactions between the CA and Cd(NO3)2·4H2O were investigated by IR spectroscopy. The change of ionic species in Cd(NO3)2·4H2O was analyzed by Raman spectroscopy.
Tuning Functional Behavior of Humic Acids through Interactions with Stöber Silica Nanoparticles
Authors: Pota Giulio , Venezia Virginia , Vitiello Giuseppe , Di Donato Paola , Mollo Valentina , Costantini Aniello , Avossa Joshua , Nuzzo Assunta , Piccolo Alessandro , Silvestri Brigida , Luciani Giuseppina
Published: April 2020

Humic acids (HA) exhibit fascinating multifunctional features, yet degradation phenomena as well as poor stability in aqueous environments strongly limit their use. Inorganic nanoparticles are emerging as a powerful interface for the development of robust HA bio-hybrid materials with enhanced chemical stability and tunable properties. Hybrid organic-inorganic SiO2/HA nanostructures were synthesized via an in-situ sol-gel route, exploiting both physical entrapment and chemical coupling. The latter was achieved through amide bond formation between carboxyl groups of HA and the amino group of 3-aminopropyltriethoxysilane (APTS), as confirmed by Fourier-Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopy. Monodisperse hybrid nanoparticles about 90 nm in diameter were obtained in both cases, yet Electron Paramagnetic Resonance (EPR) spectroscopy highlighted the different supramolecular organization of HA. The altered HA conformation was reflected in different antioxidant properties of the conjugated nanoparticles that, however, resulted in being higher than for pure HA. Our findings proved the key role of both components in defining the morphology of the final system, as well as the efficacy of the ceramic component in templating the HA supramolecular organization and consequently tuning their functional features, thus defining a green strategy for bio-waste valorization.
Coupling Dynamic Covalent Bonds and Ionic Crosslinking Network to Promote Shape Memory Properties of Ethylene-vinyl Acetate Copolymers
Authors: Wu Wenjing , Narayana Kurup Sreeni , Ellingford Christopher , Li Jie , Wan Chaoying
Published: April 2020

Dynamic crosslinking networks based on Diels–Alder (DA) chemistry and ionic interactions were introduced to maleic anhydride modified ethylene-vinyl acetate copolymer (mEVA) via in situ melt processing. The dual dynamic crosslinking networks were characterized by temperature-dependent FTIR, and the effects on the shape memory properties of mEVA were evaluated with dynamic mechanical thermal analysis and cyclic tensile testing. A crosslinking density was achieved at 2.36 × 10−4 mol·cm−3 for DA-crosslinked mEVA; as a result, the stress at 100% extension was increased from 3.8 to 5.6 MPa, and tensile strength and elongation at break were kept as high as 30.3 MPa and 486%, respectively. The further introduction of 10 wt % zinc methacrylate increased the dynamic crosslinking density to 3.74 × 10−4 mol·cm−3 and the stress at 100% extension to 9.0 MPa, while providing a tensile strength of 28.4 MPa and strain at break of 308%. The combination of reversible DA covalent crosslinking and ionic network in mEVA enabled a fixing ratio of 76.4% and recovery ratio of 99.4%, exhibiting an enhanced shape memory performance, especially at higher temperatures. The enhanced shape memory and mechanical performance of the dual crosslinked mEVA showed promising reprocessing and recycling abilities of the end-of-life products in comparison to traditional peroxide initiated covalent crosslinked counterparts.
Thermal and Torrefaction Characteristics of a Small-Scale Rotating Drum Reactor
Authors: Soponpongpipat Nitipong , Nanetoe Suwat , Comsawang Paisan
Published: April 2020

The small-scale rotating drum reactor (SS-RDR) was designed and constructed without using purge gas for the purpose of household application. The thermal and torrefaction characteristics of SS-RDR were studied and compared with other reactor types. It was found that the heat loss at the reactor wall and heat loss from exhaust gas of the SS-RDR were in the range of 6.3–12.4% and 27.9–42.8%, respectively. The increase of flue gas temperature resulted in the decrease of heat loss at the reactor wall and the increase of heat loss from exhaust gas. The heating rate of the SS-RDR was in the range of 7.3–21.4 °C/min. The higher heating value (HHV) ratio, mass yield, and energy yield ofthe SS-RDR were in the range of 1.2–1.6, 35.0–81.0%, and 56.2–96.5%, respectively. A comparison of torrefaction characteristics of various reactor types on HHV ratio-mass yield-iso-energy yield diagram indicated that the torrefaction characteristics of the SS-RDR were better than that of the rotating drum reactor with purge gas.
Investigations on Ozone-Based and UV/US-Assisted Synergistic Digestion Methods for the Determination of Total Dissolved Nitrogen in Waters
Authors: Sun Xiaofang , Chen Huixuan , Liu Zhengyu , Zhou Mengfei , Cai Yijun , Pan Haitian , Xia Luyue
Published: April 2020

Over the past two decades, the alkaline persulfate oxidation (PO) with thermal and/or ultraviolet (UV) assisted digestion method has been widely used for digestion of nitrogen containing compounds (N-compounds) in water quality routine analysis in laboratory or on-line analysis, due to its simple principle, high conversion rate, high percent recovery, low-cost. However, this digestion method still has some inevitable problems such as complex operations, high contamination potential, batch N blanks, higher reaction temperature (120–124 °C) and time-consuming (30–60 min). In this study, ozone (O3) was selected as the oxidant for digestion of N-compounds through analysis and comparison firstly. Secondly, we proposed and compared the UV and/or ultrasound (US) combined with ozone (UV/O3, US/O3 and UV/US/O3) synergistic digestion methods based on O3 with sole O3 oxidation method on digestion efficiency (digestion time and conversion rate) of standard N-compounds. Simultaneously, the influence of reaction temperature, pH of water sample, concentration of O3 and mass flow rate, UV intensity, US frequency and power on digestion efficiency were investigated, and then the optimum parameters for digestion system were obtained. Experimental results indicated that UV radiation can effectively induce and promote the decomposition and photolysis of O3 in water to generate hydroxyl radicals (•OH), while US can promote the diffusion and dissolution of O3 in water and intensify the gas-liquid mass transfer process for the reaction system. Meanwhile, results showed that the UV/US/O3 synergistic digestion method had the best digestion efficiency under the optimum conditions: water sample volume, 10 mL; pH of water sample, 11; O3 mass flow rate, 3200 mg/h; reaction temperature, 30 °C; digestion time, 25 min; UV lamp power, 18 W; distance between UV lamp and reactor, 2 cm; US frequency, 20 kHz; US power, 75 W. The conversion rate (CR) of synthetic wastewater samples varied from 99.6% to 101.4% for total dissolved nitrogen (TDN) in the range of 1.0~4.0 mg/L. The UV/US/O3 synergistic digestion method would be an effective and potential alternative for digestion of N-compounds in water quality routine analysis in laboratory or on-line analysis.
Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering
Authors: Yu Chun-Ta , Wang Fu-Ming , Liu Yen-Ting , Ng Hooi Yee , Jhong Yi-Rong , Hung Chih-Hung , Chen Yi-Wen
Published: April 2020

Bone has a complex hierarchical structure with the capability of self-regeneration. In the case of critical-sized defects, the regeneration capabilities of normal bones are severely impaired, thus causing non-union healing of bones. Therefore, bone tissue engineering has since emerged to solve problems relating to critical-sized bone defects. Amongst the many biomaterials available on the market, calcium silicate-based (CS) cements have garnered huge interest due to their versatility and good bioactivity. In the recent decade, scientists have attempted to modify or functionalize CS cement in order to enhance the bioactivity of CS. Reports have been made that the addition of mesoporous nanoparticles onto scaffolds could enhance the bone regenerative capabilities of scaffolds. For this study, the main objective was to reuse gelatin from fish wastes and use it to combine with bone morphogenetic protein (BMP)-2 and Sr-doped CS scaffolds to create a novel BMP-2-loaded, hydrogel-based mesoporous SrCS scaffold (FGSrB) and to evaluate for its composition and mechanical strength. From this study, it was shown that such a novel scaffold could be fabricated without affecting the structural properties of FGSr. In addition, it was proven that FGSrB could be used for drug delivery to allow stable localized drug release. Such modifications were found to enhance cellular proliferation, thus leading to enhanced secretion of alkaline phosphatase and calcium. The above results showed that such a modification could be used as a potential alternative for future bone tissue engineering research.
Minimize the Route Length Using Heuristic Method Aided with Simulated Annealing to Reinforce Lean Management Sustainability
Authors: Abed Ahmed M. , Elattar Samia
Published: April 2020

Cost reduction is a cornerstone of the Lean administration’s sustainability through modify its algorithms scheme to become multi-useful. This paper focuses on control “movement” waste, to minimize pipeline, cabling and sewerage network deployments time, to avoid demurrages (i.e., constructor sectors) and quickens planning through two stages. The first belongs to the build constrained hybridization of published heuristic routing methods (e.g., S-Shape, Mid-point, Largest-Gap, Return, Ascending, FLA-5, FLA-6 [Flow Line Analysis], and Composite) to select the shortest path that serves many locations (i.e., Plan-A), while allowing for the modification of these locations during service (i.e., Plan-B). The new locations are grouped into two clusters, the first of which lay on the shortest preferred path, while the second cluster contains locations that do not lay on the preferred path and are therefore moved on the backlogs-list, then use Simulated Annealing when to serve them. Finally, the impact of the selected performance is investigated after studying its correlation with another published effective one under cost considerations. The computational results of proposed Minimize-Route-Length aided with simulated annealing (MRL-SA) significantly outperform others in terms of the performance of the routing heuristics and total costs and develop the Last Planner System, which has a good reputation in construction projects and approve the proposed algorithm to maintain its competitiveness sustainability.
Kwanŭm (Avalokiteśvara) Divination: Buddhist Reconciliation with Confucianism in the Late Chosŏn
Authors: Kim Seong U. K.
Published: April 2020

This paper looks at the interactions between Buddhism and Confucianism in the late Chosŏn era through a case study of Kwanŭm (Avalokiteśvara) divination and, thereby, attempts to demonstrate how Buddhist monks navigated their religion in a Confucian-dominant society. In particular, it discusses how Chosŏn Buddhist monks incorporated Confucian ethical values into the practice of Avalokiteśvara divination, which developed in the late Chosŏn era, based on the Kwanŭm yŏnggwa, the first Korean Buddhist manual for Avalokiteśvara divination. The unique amalgam of the Avalokiteśvara cult and divination practices became popular in this period by embracing the fulfillment of the Confucian duty of filial piety and sincerity as its essential element. In particular, the Confucian practice served as a deciding factor to receive the bodhisattva’s blessings in this divination. The introduction of Avalokiteśvara divination, therefore, reveals another strategy of the Buddhist community to demonstrate its devotion by incorporating the ideology of the Confucian ruling class in the late Chosŏn era.
Free to Die: A Lutheran-Relational Approach to Medical Assistance in Dying
Authors: Driedger Hesslein Kayko
Published: April 2020

In 2016, Canada legalized Bill C-14, which removed from the Criminal Code medical assistance in dying (MAID) under certain circumstances. In essence, the Bill legalized what is commonly described as euthanasia, under the provisions that the patient has a terminal medical diagnosis, anticipates extreme suffering, is at least 18 years old and still maintains their own medical power of attorney, has received the same diagnosis from two separate doctors, and requests such a procedure without duress. The bill exempts doctors and nurses from culpability in murder, along with those aiding the medical staff. The bill replaces sections of the Criminal Code that criminalize death by suicide. In this article, I first review the theological and historical interpretation of suicide within the Christian church. I then offer a specifically Lutheran feminist framework of our baptism into death, Luther’s explanation of the First Commandment, and Christian freedom to affirm Christians’ faithful decisions in seeking medical assistance in dying.
Rejecting “Controversial” Issues in Education: A Case Study of Ultra-Orthodox Jewish Schools in Belgium
Authors: Franken Leni , Levrau François
Published: April 2020

In liberal democracies, fundamental rights and freedoms can conflict, and if they do, it is not always clear which right the state should prioritize. Should the right of parents to choose education in line with their own convictions prevail, or should the right of children to be prepared for a future life in a liberal democratic society be given more moral weight? While the former might lead to establishing and subsidizing orthodox religious schools, the latter implies “liberal”, “autonomy-facilitating” education. In order to make this tension concrete, we focus on a case study of an ultra-orthodox Jewish (Haredi) school in Flanders (Belgium), where “controversial issues” are excluded from the curriculum and where education is not fully in line with the core principles of “liberal education”. Subsequently, we explore the legal educational context in liberal democracies, with a particular focus on the freedom of religion and education. Then, we scrutinize several arguments for or against ultra-orthodox faith-based schools. We conclude that there are no convincing arguments for state support for these kinds of schools and that the recent Flemish policy of homeschooling might be a reasonable alternative, wherein a balance is found between children’s rights and parental rights.
A Tail Dependence-Based MST and Their Topological Indicators in Modeling Systemic Risk in the European Insurance Sector
Authors: Denkowska Anna , Wanat Stanisław
Published: April 2020

In the present work, we analyze the dynamics of indirect connections between insurance companies that result from market price channels. In our analysis, we assume that the stock quotations of insurance companies reflect market sentiments, which constitute a very important systemic risk factor. Interlinkages between insurers and their dynamics have a direct impact on systemic risk contagion in the insurance sector. Herein, we propose a new hybrid approach to the analysis of interlinkages dynamics based on combining the copula-DCC-GARCH model and minimum spanning trees (MST). Using the copula-DCC-GARCH model, we determine the tail dependence coefficients. Then, for each analyzed period we construct MST based on these coefficients. The dynamics are analyzed by means of the time series of selected topological indicators of the MSTs in the years 2005–2019. The contribution to systemic risk of each institution is determined by analyzing the deltaCoVaR time series using the copula-DCC-GARCH model. Our empirical results show the usefulness of the proposed approach to the analysis of systemic risk (SR) in the insurance sector. The times series obtained from the proposed hybrid approach reflect the phenomena occurring in the market. We check whether the analyzed MST topological indicators can be considered as systemic risk predictors.