bluuebunny/crossref_metadata_2025 huggingface dataset:
An Automatic 3D Point Cloud Registration Method Based on Biological Vision
Authors: Liu Jinbo , Guo Pengyu , Sun Xiaoliang
Published: May 2021

When measuring surface deformation, because the overlap of point clouds before and after deformation is small and the accuracy of the initial value of point cloud registration cannot be guaranteed, traditional point cloud registration methods cannot be applied. In order to solve this problem, a complete solution is proposed, first, by fixing at least three cones to the target. Then, through cone vertices, initial values of the transformation matrix can be calculated. On the basis of this, the point cloud registration can be performed accurately through the iterative closest point (ICP) algorithm using the neighboring point clouds of cone vertices. To improve the automation of this solution, an accurate and automatic point cloud registration method based on biological vision is proposed. First, the three-dimensional (3D) coordinates of cone vertices are obtained through multi-view observation, feature detection, data fusion, and shape fitting. In shape fitting, a closed-form solution of cone vertices is derived on the basis of the quadratic form. Second, a random strategy is designed to calculate the initial values of the transformation matrix between two point clouds. Then, combined with ICP, point cloud registration is realized automatically and precisely. The simulation results showed that, when the intensity of Gaussian noise ranged from 0 to 1 mr (where mr denotes the average mesh resolution of the models), the rotation and translation errors of point cloud registration were less than 0.1° and 1 mr, respectively. Lastly, a camera-projector system to dynamically measure the surface deformation during ablation tests in an arc-heated wind tunnel was developed, and the experimental results showed that the measuring precision for surface deformation exceeded 0.05 mm when surface deformation was smaller than 4 mm.
A Review of Modified Steel Slag Application in Catalytic Pyrolysis, Organic Degradation, Electrocatalysis, Photocatalysis, Transesterification and Carbon Capture and Storage
Authors: Wang Fu-Ping , Liu Tian-Ji , Cai Shuang , Gao Di , Yu Qing , Wang Xiao-Man , Wang Yi-Tong , Zeng Ya-Nan , Li Jun-Guo
Published: May 2021

As a by-product of the iron and steel industry, steel slag is rich in catalytically active substances and can therefore be used as a solid catalyst. Many studies have shown that the application potential of steel slag in catalysis is huge, which provides new development space for its application, thereby increasing its additional utilization value. This article primarily reviews the research progress in catalytic fields such as catalytic pyrolysis, organic degradation, electrocatalysis, photocatalysis, transesterification, and carbon capture and storage, as well as the modification methods of steel slag. The catalytic performance of the modified steel slag has been further improved, and it has the meaningful characteristics of high efficiency, cleanliness, and low costs.
Early Treatment with a Slow Maxillary Ni–Ti Leaf Springs Expander
Authors: Vella Massimiliano , Cressoni Paolo , Tripicchio Cinzia , Mainardi Eleonora , Esposito Luca
Published: May 2021

The aim of this brief report is to analyse the available literature on the clinical outcomes of a particular appliance for slow maxillary expansion that consists of one or more nickel–titanium springs. Materials and methods: The main medical databases (Scopus, Web of Sciences, Pubmed and Google Scholar) were scanned up to January 2020 using “slow maxillary expan*”, “slow palatal expan*”, “leaf expander” and “NiTi Palatal Expander” as keywords. Skeletal changes in the maxilla after expansion with the Leaf Expander (L.E.) or similar appliances were taken into consideration while reviewing relevant manuscripts. The review focuses on the comparison between the L.E. and conventional expanders (i.e., Haas and Hyrax) regarding the increase in both the distance between the palatal cusps of the upper first molars and the distance between the palatal cusps of the upper second deciduous molars, as well as the increment of nasal structures and pain connected to expansion procedures. Results: Bibliographic research retrieved 32 articles that were considered eligible for the present study. The limited number of articles currently available in international medical databases is allegedly partly due to the fact that these expanders are currently produced by only one patent holder company, which affects its diffusion. Conclusion: Despite the reduced number of published articles, due to the recent introduction of the L.E. device, most of the authors have found that the effects of the L.E. device are clinically and radiographically comparable to those achievable with the rapid palatal expander.
Dynamic Test Measurements and Simulation on a Series Wound DC Motor
Authors: Szántó Attila , Kiss János , Mankovits Tamás , Szíki Gusztáv Áron
Published: May 2021

Previously, a dynamic model and a simulation program for series wound DC motors (SWDCM) were developed in MATLAB/Simulink for modelling them in electric vehicles and mechatronic systems. The electromagnetic characteristics of the motor (electric resistances, dynamic inductances), which serve as input parameters of the program, were also measured. Additionally, locked rotor response measurements were performed to test the accuracy of the measured electromagnetic characteristics. This paper presents the experimental procedure and the results of dynamics test measurements that were performed on the same motor, including the procedure for the determination of the necessary input dynamic parameters for the simulation. While the motor spins up from rest, the intensity of the electric current and the angular speed of the rotor are measured. Finally, the simulation and dynamic test results are compared to check the proper operation of the simulation program.
3D Digital Adaptive Thorax Modelling of Peoples with Spinal Disabilities: Applications for Performance Clothing Design
Authors: Mosleh Sara , Abtew Mulat Alubel , Bruniaux Pascal , Tartare Guillaume , Xu Yukang , Chen Yan
Published: May 2021

Peoples with spinal disability face a huge problem in the design and development of ergonomically fitted and comfortable clothing. Various research studies on the design and developments of functional clothing for scoliosis patients consider their morphological shapes. However, developing appropriate models of the complicated and deformed anatomical shape of the patient in 3D digitization technologies makes it possible to design a comfortable and fitted garment. The current paper proposes a method for developing a fully parametric 3D adaptive model of the thorax of a patient suffering from scoliosis. The model is designed from the spine and follows the deformation of the spine to adapt the thorax skeleton according to the temporal evolution of the spinal column deformation. The integration of the model of the thorax, adjusted to the patient’s data, enables the chain of acquisition, processing, and global model to be validated. The fit of the model could be improved for the different bones and it is possible to modify the angles of the spine to see the evolution of the disease. The developed model greatly helps to further detect anthropometric points from certain bone parts of the skeleton to design a basic bodice adapted to the patient’s evolving morphology.
Mobile Augmented Reality Technologies for Autism Spectrum Disorder Interventions: A Systematic Literature Review
Authors: Lian Xiaojie , Sunar Mohd Shahrizal
Published: May 2021

Over the past decade, enhanced computing capabilities and mobile technologies have begotten the upsurge of innovative mobile health (mHealth) solutions, and many research efforts have occurred recently in the area of technology-based interventions (TBI) for autism spectrum disorders (ASD). Mobile augmented reality (MAR) refers to AR systems that use the handheld mobile device medium (mobile phones, tablets or smart glasses). This article reports the results of a systematic review undertaken on the use of MAR for ASD-related skills learning from the year 2010. It aims to provide an insight into the current state of research on MAR interventions and to provide guidance to relevant designers and researchers. We searched seven databases and retrieved 625 articles initially. After exclusion and screening, 36 articles were reviewed reporting on using MAR to improve various skills of children and adolescents with ASD, and 10 research questions related to PICO (P: Population, I: Intervention, C: Comparison, O: Outcomes) were addressed. This study identifies challenges that still exist in the research efforts towards the development of applications exploiting the MAR for ASD interventions: technology issues, research design consideration, subjective assessment etc. The studies examined suggest researchers should focus on users and improve the quality of the MAR app. In addition, more effective research methods and evaluation methods could be involved in future studies to facilitate the development of MAR intervention applications.
Simulation of Aerosol Evolution within Background Pollution for Nucleated Vehicle Exhaust via TEMOM
Authors: Tu Can , Liu Yueyan , Wu Taiquan , Yu Mingzhou
Published: May 2021

This work is intended to study the effect of background particles on vehicle emissions in representative realistic atmospheric environments. The coupling of Reynolds-Averaged Navier–Stokes equation (RANS) and Taylor-series Expansion Method Of Moments (TEMOM) is performed to track the emissions of the vehicle and simulating the evolution of the matters. The transport equation of mass, momentum, heat, and the first three orders of moments are taken into account with the effect of binary homogeneous nucleation, Brownian coagulation, condensation, and thermophoresis. The parameterization model is utilized for nucleation. The measured data for Beijing’s particle size distribution under both polluted and nonpolluted conditions are utilized as background particles. The relationship between the macroscopic measurement results and the microscopic dynamic process is analyzed by comparing the variation trend of several physical quantities in the process of aerosol evolution. It is found with an increase of background particle concentration, the nucleation is inhibited, which is consistent with the existing studies.
Effects of Preharvest Methyl Jasmonate Elicitation and Electrical Stimulation on Camptothecin Production by In Vitro Plants of Ophiorrhiza ridleyana Craib
Authors: Pisitpaibool Supakit , Sukrong Suchada , Kanjanaprapakul Kijchai , Phisalaphong Muenduen
Published: May 2021

To enhance plant camptothecin (CPT) production in vitro, 5-month-old Ophiorrhiza ridleyana Craib plant cultures were treated with solutions of methyl jasmonate (MeJA) dissolved in ethanol, which were applied to the surface of the solid culture medium. It was demonstrated that the maximum CPT content in the tissue-cultured plants was achieved after 12 h elicitation with 50 µM MeJA. The mean CPT contents in roots and stems were 50.8 and 67.0 µg/g DW, respectively, which were approximately 1.8- and 2.6-fold higher, respectively, than those of the control. However, MeJA elicitation showed no significant effect on CPT accumulation in O. ridleyana leaves. Moreover, it was found that direct electric current (DC) stimulation also significantly increased CPT accumulation in O. ridleyana. The treatment with DC at 20 mA for 3 min of stimulation enhanced 3-fold the CPT content in roots, stems, and leaves to 41.9, 36.0 and 19.6 µg/g DW, respectively, which were approximately 1.5-, 1.7- and 1.4-fold higher, respectively, as compared to those of the control. The results demonstrate that preharvest treatment by MeJA elicitation and electrical stimulation can be beneficial for secondary metabolite production of CPT in tissue-culture plants of O. ridleyana.
The UWB Radar Application in the Aviation Security Systems
Authors: Džunda Milan , Dzurovčin Peter , Kaľavský Peter , Korba Peter , Cséfalvay Zoltán , Hovanec Michal
Published: May 2021

In the process of our research, we have identified new methods of processing ultra-wide-band (UWB) radar signals and possibilities of the UWB radar use in aviation security systems. We paid our main attention to finding new algorithms for tracking the movement of a person behind an obstacle using the UWB radar. Such UWB radar application is typical for tracking the movement of people behind obstacles in case of security forces intervention at an airport. In the research process, we used methods of analysis, synthesis, and measured data from the performed experiment. The main contribution of the paper is the development of new algorithms for locating the movement of a person behind an obstacle using a straight- line method in the case of using two independent UWB radar systems. The article did not examine the accuracy of determining the position of a person behind the obstacle. We found that when applying the Kalman filter after signal processing by the straight-line method, the trajectory of the person’s movement behind the obstacle was smoother. The results of processing the measurement signals of UWB radar by the linear method have shown that this method is applicable to tracking a person behind an obstacle and can be used in aviation security systems.
On Finding the Right Sampling Line Height through a Parametric Study of Gas Dispersion in a NVB
Authors: Doumbia E. Moustapha , Janke David , Yi Qianying , Zhang Guoqiang , Amon Thomas , Kriegel Martin , Hempel Sabrina
Published: May 2021

The tracer gas method is one of the common ways to evaluate the air exchange rate in a naturally ventilated barn. One crucial condition for the accuracy of the method is that both considered gases (pollutant and tracer) are perfectly mixed at the points where the measurements are done. In the present study, by means of computational fluids dynamics (CFD), the mixing ratio NH3/CO2 is evaluated inside a barn in order to assess under which flow conditions the common height recommendation guidelines for sampling points (sampling line and sampling net) of the tracer gas method are most valuable. Our CFD model considered a barn with a rectangular layout and four animal-occupied zones modeled as a porous medium representing pressure drop and heat entry from lying and standing cows. We studied three inflow angles and six combinations of air inlet wind speed and temperatures gradients covering the three types of convection, i.e., natural, mixed, and forced. Our results showed that few cases corresponded to a nearly perfect gas mixing ratio at the currently common recommendation of at least a 3 m measurement height, while the best height in fact lied between 1.5 m and 2.5 m for most cases.
Spindle Error Movements and Their Measurement
Authors: Chrzanowski Jarosław , Sałaciński Tadeusz , Skiba Paweł
Published: May 2021

The spindle of a machine tool is an important component of the machine. Its condition affects not only operation of the machine tool but also, above all, the quality of the workpiece. This paper explains the terminology and describes the research methods used to measure the spindle error movements of cutting machine tools. This article explains the impact of spindle motion inaccuracies on the quality of workpieces, quotes the requirements of European and American standards, and describes the current state and directions of development of the presented methods. Our own research work in this field is also presented.
A New Approach to Group Multi-Objective Optimization under Imperfect Information and Its Application to Project Portfolio Optimization
Authors: Fernández Eduardo , Rangel-Valdez Nelson , Cruz-Reyes Laura , Gomez-Santillan Claudia
Published: May 2021

This paper addresses group multi-objective optimization under a new perspective. For each point in the feasible decision set, satisfaction or dissatisfaction from each group member is determined by a multi-criteria ordinal classification approach, based on comparing solutions with a limiting boundary between classes “unsatisfactory” and “satisfactory”. The whole group satisfaction can be maximized, finding solutions as close as possible to the ideal consensus. The group moderator is in charge of making the final decision, finding the best compromise between the collective satisfaction and dissatisfaction. Imperfect information on values of objective functions, required and available resources, and decision model parameters are handled by using interval numbers. Two different kinds of multi-criteria decision models are considered: (i) an interval outranking approach and (ii) an interval weighted-sum value function. The proposal is more general than other approaches to group multi-objective optimization since (a) some (even all) objective values may be not the same for different DMs; (b) each group member may consider their own set of objective functions and constraints; (c) objective values may be imprecise or uncertain; (d) imperfect information on resources availability and requirements may be handled; (e) each group member may have their own perception about the availability of resources and the requirement of resources per activity. An important application of the new approach is collective multi-objective project portfolio optimization. This is illustrated by solving a real size group many-objective project portfolio optimization problem using evolutionary computation tools.
Ranking Road Sections Based on MCDM Model: New Improved Fuzzy SWARA (IMF SWARA)
Authors: Vrtagić Sabahudin , Softić Edis , Subotić Marko , Stević Željko , Dordevic Milan , Ponjavic Mirza
Published: May 2021

Traffic management is a significantly difficult and demanding task. It is necessary to know the main parameters of road networks in order to adequately meet traffic management requirements. Through this paper, an integrated fuzzy model for ranking road sections based on four inputs and four outputs was developed. The goal was to determine the safety degree of the observed road sections by the methodology developed. The greatest contribution of the paper is reflected in the development of the improved fuzzy step-wise weight assessment ratio analysis (IMF SWARA) method and integration with the fuzzy measurement alternatives and ranking according to the compromise solution (fuzzy MARCOS) method. First, the data envelopment analysis (DEA) model was applied, showing that three road sections have a high traffic risk. After that, IMF SWARA was applied to determine the values of the weight coefficients of the criteria, and the fuzzy MARCOS method was used for the final ranking of the sections. The obtained results were verified through a three-phase sensitivity analysis with an emphasis on forming 40 new scenarios in which input values were simulated. The stability of the model was proven in all phases of sensitivity analysis.
Bone Marrow Multipotent Mesenchymal Stromal Cells as Autologous Therapy for Osteonecrosis: Effects of Age and Underlying Causes
Authors: El-Jawhari Jehan J , Ganguly Payal , Jones Elena , Giannoudis Peter V
Published: May 2021

Bone marrow (BM) is a reliable source of multipotent mesenchymal stromal cells (MSCs), which have been successfully used for treating osteonecrosis. Considering the functional advantages of BM-MSCs as bone and cartilage reparatory cells and supporting angiogenesis, several donor-related factors are also essential to consider when autologous BM-MSCs are used for such regenerative therapies. Aging is one of several factors contributing to the donor-related variability and found to be associated with a reduction of BM-MSC numbers. However, even within the same age group, other factors affecting MSC quantity and function remain incompletely understood. For patients with osteonecrosis, several underlying factors have been linked to the decrease of the proliferation of BM-MSCs as well as the impairment of their differentiation, migration, angiogenesis-support and immunoregulatory functions. This review discusses the quality and quantity of BM-MSCs in relation to the etiological conditions of osteonecrosis such as sickle cell disease, Gaucher disease, alcohol, corticosteroids, Systemic Lupus Erythematosus, diabetes, chronic renal disease and chemotherapy. A clear understanding of the regenerative potential of BM-MSCs is essential to optimize the cellular therapy of osteonecrosis and other bone damage conditions.
A Bittersweet Computational Journey among Glycosaminoglycans
Authors: Paiardi Giulia , Milanesi Maria , Wade Rebecca C. , D’Ursi Pasqualina , Rusnati Marco
Published: May 2021

Glycosaminoglycans (GAGs) are linear polysaccharides. In proteoglycans (PGs), they are attached to a core protein. GAGs and PGs can be found as free molecules, associated with the extracellular matrix or expressed on the cell membrane. They play a role in the regulation of a wide array of physiological and pathological processes by binding to different proteins, thus modulating their structure and function, and their concentration and availability in the microenvironment. Unfortunately, the enormous structural diversity of GAGs/PGs has hampered the development of dedicated analytical technologies and experimental models. Similarly, computational approaches (in particular, molecular modeling, docking and dynamics simulations) have not been fully exploited in glycobiology, despite their potential to demystify the complexity of GAGs/PGs at a structural and functional level. Here, we review the state-of-the art of computational approaches to studying GAGs/PGs with the aim of pointing out the “bitter” and “sweet” aspects of this field of research. Furthermore, we attempt to bridge the gap between bioinformatics and glycobiology, which have so far been kept apart by conceptual and technical differences. For this purpose, we provide computational scientists and glycobiologists with the fundamentals of these two fields of research, with the aim of creating opportunities for their combined exploitation, and thereby contributing to a substantial improvement in scientific knowledge.
Plasma Extracellular Vesicle α-Synuclein Level in Patients with Parkinson’s Disease
Authors: Chung Chen-Chih , Chan Lung , Chen Jia-Hung , Hung Yi-Chieh , Hong Chien-Tai
Published: May 2021

Background: The most established pathognomonic protein of Parkinson’s disease (PD), α-synuclein, is extensively investigated for disease diagnosis and prognosis; however, investigations into whether the free form of α-synuclein in the blood functions as a PD biomarker have not been fruitful. Extracellular vesicles (EVs) secreted from cells and present in blood transport molecules are novel platforms for biomarker identification. In blood EVs, α-synuclein originates predominantly from the brain without the interference of the blood–brain barrier. The present study investigated the role of plasma EV-borne α-synuclein as a biomarker of PD. Methods: Patients with mild to moderate stages of PD (n = 116) and individuals without PD (n = 46) were recruited to serve as the PD study group and the control group, respectively. Plasma EVs were isolated, and immunomagnetic reduction–based immunoassay was used to assess EV α-synuclein levels. Conventional statistical analysis was performed using SPSS 25.0, and p < 0.05 was considered significant. Results: Compared with controls, we observed significantly lower plasma EV α-synuclein levels in the patients with PD (PD: 56.0 ± 3.7 fg/mL vs. control: 74.5 ± 4.3 fg/mL, p = 0.009), and the significance remained after adjustment for age and sex. Plasma EV α-synuclein levels in the patients with PD did not correlate with age, disease duration, Part I and II scores of the Unified Parkinson’s Disease Rating Scale (UPDRS), or the Mini-Mental State Examination scores. However, such levels were significantly correlated with UPDRS Part III score, which assesses motor dysfunction. Furthermore, the severity of akinetic-rigidity symptoms, but not tremor, was inversely associated with plasma EV α-synuclein level. Conclusion: Plasma EV α-synuclein was significantly different between the control and PD group and was associated with akinetic-rigidity symptom severity in patients with PD. This study corroborates the possible diagnostic and subtyping roles of plasma EV α-synuclein in patients with PD, and it further provides a basis for this protein’s clinical relevance and feasibility as a PD biomarker.
Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
Authors: González-García Melaine , Morales-Vicente Fidel , Pico Erbio Díaz , Garay Hilda , Rivera Daniel G. , Grieshober Mark , Raluca Olari Lia , Groß Rüdiger , Conzelmann Carina , Krüger Franziska , Zech Fabian , Prelli Bozzo Caterina , Müller Janis A. , Zelikin Alexander , Raber Heinz , Kubiczek Dennis , Rosenau Frank , Münch Jan , Stenger Steffen , Spellerberg Barbara , Franco Octavio L. , Rodriguez Alfonso Armando A. , Ständker Ludger , Otero-Gonzalez Anselmo J.
Published: May 2021

Cm-p5 is a snail-derived antimicrobial peptide, which demonstrated antifungal activity against the pathogenic strains of Candida albicans. Previously we synthetized a cyclic monomer as well as a parallel and an antiparallel dimer of Cm-p5 with improved antifungal activity. Considering the alarming increase of microbial resistance to conventional antibiotics, here we evaluated the antimicrobial activity of these derivatives against multiresistant and problematic bacteria and against important viral agents. The three peptides showed a moderate activity against Pseudomonas aeruginosa, Klebsiella pneumoniae Extended Spectrum β-Lactamase (ESBL), and Streptococcus agalactiae, with MIC values > 100 µg/mL. They exerted a considerable activity with MIC values between 25–50 µg/mL against Acinetobacter baumanii and Enterococcus faecium. In addition, the two dimers showed a moderate activity against Pseudomonas aeruginosa PA14. The three Cm-p5 derivatives inhibited a virulent extracellular strain of Mycobacterium tuberculosis, in a dose-dependent manner. Moreover, they inhibited Herpes Simplex Virus 2 (HSV-2) infection in a concentration-dependent manner, but had no effect on infection by the Zika Virus (ZIKV) or pseudoparticles of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2). At concentrations of >100 µg/mL, the three new Cm-p5 derivatives showed toxicity on different eukaryotic cells tested. Considering a certain cell toxicity but a potential interesting activity against the multiresistant strains of bacteria and HSV-2, our compounds require future structural optimization.
Synthesis, Characterization, and In Vitro Insulin-Mimetic Activity Evaluation of Valine Schiff Base Coordination Compounds of Oxidovanadium(V)
Authors: Turtoi Mihaela , Anghelache Maria , Patrascu Andrei A. , Maxim Catalin , Manduteanu Ileana , Calin Manuela , Popescu Delia-Laura
Published: May 2021

Type 2 diabetes became an alarming global health issue since the existing drugs do not prevent its progression. Herein, we aimed to synthesize and characterize a family of oxidovanadium(V) complexes with Schiff base ligands derived from L-/D-valine (val) and salicylaldehyde (sal) or o-vanillin (van) as insulin-mimetic agents and to assess their potential anti-diabetic properties. Two new oxidovanadium(V) complexes, [{VVO(R-salval)(H2O)}(μ2-O){VVO(R-salval)}] and [{VVO(R-vanval)(CH3OH)}2(μ2-O)], and their S-enantiomers were synthesized and characterized. The compounds exhibit optical activity as shown by crystallographic and spectroscopic data. The stability, the capacity to bind bovine serum albumin (BSA), the cytotoxicity against human hepatoma cell line, as well as the potential anti-diabetic activity of the four compounds are investigated. The synthesized compounds are stable for up to three hours in physiological conditions and exhibit a high capacity of binding to BSA. Furthermore, the synthesized compounds display cytocompatibility at biologically relevant concentrations, exert anti-diabetic potential and insulin-mimetic activities by inhibiting the α-amylase and protein tyrosine phosphatase activity, and a long-term increase of insulin receptor phosphorylation compared to the insulin hormone. Thus, the in vitro anti-diabetic potential and insulin-mimetic properties of the newly synthesized oxidovanadium(V) compounds, correlated with their cytocompatibility, make them promising candidates for further investigation as anti-diabetic drugs.
FRP Cables to Prestress RC Beams: State of the Art vs. a Split Wedge Anchorage System
Authors: Damiani Marco , Quadrino Attilio , Nisticò Nicola
Published: May 2021

Versatility and high performance in terms of specific stiffness and strength, as well as non-corrosive sensitivity, make FRP (Fiber-Reinforced Polymer) cables a viable alternative to steel ones in the development of prestressing systems. On the other hand, the orthotropic and brittle nature of FRPs could trigger a premature failure of the cable in the anchorage system, for which several solutions have been proposed so far in civil structural applications. In this context, after a preliminary state of the art, the work introduces a split wedge anchorage for FRP (ϕ = 12 mm) cables proposing two different solutions for steel wedges having the external surface: either (1) a constant (3 degrees) slope or (2) a double slope obtained by shaping it with an angle of 3.0 degrees before and then of 3.1 degrees along the most tapered part. The goal was to exploit the nominal cable capacity (257 kN), avoiding stress peaks that cause its premature failure. The proposed solutions have been experimentally tested and, as far as the double angle solution is concerned, the failure loads were equal to 222 and 257 kN, denoting that the proposed solution can reach the cable capacity. Clearly, further investigations are needed to check the variability of the results and eventually improve the system.
Announcement for “Buildings Outstanding Reviewer Award 2020” Winners
Authors:   Buildings Editorial Office
Published: May 2021

Peer review is an essential part of the publication process, ensuring that Buildings maintains high-quality standards for its published papers [...]
Chondrosarcoma-from Molecular Pathology to Novel Therapies
Authors: Zając Agnieszka E. , Kopeć Sylwia , Szostakowski Bartłomiej , Spałek Mateusz J. , Fiedorowicz Michał , Bylina Elżbieta , Filipowicz Paulina , Szumera-Ciećkiewicz Anna , Tysarowski Andrzej , Czarnecka Anna M. , Rutkowski Piotr
Published: May 2021

Chondrosarcoma (CHS) is the second most common primary malignant bone sarcoma. Overall survival and prognosis of this tumor are various and often extreme, depending on histological grade and tumor subtype. CHS treatment is difficult, and surgery remains still the gold standard due to the resistance of this tumor to other therapeutic options. Considering the role of differentiation of CHS subtypes and the need to develop new treatment strategies, in this review, we introduced a multidisciplinary characterization of CHS from its pathology to therapies. We described the morphology of each subtype with the role of immunohistochemical markers in diagnostics of CHS. We also summarized the most frequently mutated genes and genome regions with altered pathways involved in the pathology of this tumor. Subsequently, we discussed imaging methods and the role of currently used therapies, including surgery and the limitations of chemo and radiotherapy. Finally, in this review, we presented novel targeted therapies, including those at ongoing clinical trials, which can be a potential future target in designing new therapeutics for patients with CHS.
BRAF/EZH2 Signaling Represses miR-129-5p Inhibition of SOX4 Thereby Modulating BRAFi Resistance in Melanoma
Authors: Gebhardt Kathleen , Edemir Bayram , Groß Elisabeth , Nemetschke Linda , Kewitz-Hempel Stefanie , Moritz Rose K. C. , Sunderkötter Cord , Gerloff Dennis
Published: May 2021

Many melanomas are associated with activating BRAF mutation. Targeted therapies by inhibitors of BRAF and MEK (BRAFi, MEKi) show marked antitumor response, but become limited by drug resistance. The mechanisms for this are not fully revealed, but include miRNA. Wishing to improve efficacy of BRAFi and knowing that certain miRNAs are linked to resistance to BRAFi, we wanted to focus on miRNAs exclusively associated with response to BRAFi. We found increased expression of miR-129-5p during BRAFi treatment of BRAF- mutant melanoma cells. Parallel to emergence of resistance we observed mir-129-5p expression to become suppressed by BRAF/EZH2 signaling. In functional analyses we revealed that miR-129-5p acts as a tumor suppressor as its overexpression decreased cell proliferation, improved treatment response and reduced viability of BRAFi resistant melanoma cells. By protein expression analyses and luciferase reporter assays we confirmed SOX4 as a direct target of mir-129-5p. Thus, modulation of the miR-129-5p-SOX4 axis could serve as a promising novel strategy to improve response to BRAFi in melanoma.
Combining Radiation Therapy with ALK Inhibitors in Anaplastic Lymphoma Kinase-Positive Non-Small Cell Lung Cancer (NSCLC): A Clinical and Preclinical Overview
Authors: Antoni Delphine , Burckel Hélène , Noel Georges
Published: May 2021

Over the past years, the identification of genetic alterations in oncogenic drivers in non-small cell lung cancer (NSCLC) has significantly and favorably transformed the outcome of patients who can benefit from targeted therapies such as tyrosine kinase inhibitors. Among these genetic alterations, anaplastic lymphoma kinase (ALK) rearrangements were discovered in 2007 and are present in 3–5% of patients with NSCLC. In addition, radiotherapy remains one of the cornerstones of NSCLC treatment. Moreover, improvements in the field of radiotherapy with the use of hypofractionated or ablative stereotactic radiotherapy have led to a better outcome for localized or oligometastatic NSCLC. To date, the effects of the combination of ALK inhibitors and radiotherapy are unclear in terms of safety and efficacy but could potently improve treatment. In this manuscript, we provide a clinical and preclinical overview of combining radiation therapy with ALK inhibitors in anaplastic lymphoma kinase-positive non-small cell lung cancer.
The Individual Effects of Cyclin-Dependent Kinase Inhibitors on Head and Neck Cancer Cells—A Systematic Analysis
Authors: Schoenwaelder Nina , Salewski Inken , Engel Nadja , Krause Mareike , Schneider Björn , Müller Michael , Riess Christin , Lemcke Heiko , Skorska Anna , Grosse-Thie Christina , Junghanss Christian , Maletzki Claudia
Published: May 2021

Cyclin-dependent kinase inhibitors (CDKi´s) display cytotoxic activity against different malignancies, including head and neck squamous cell carcinomas (HNSCC). By coordinating the DNA damage response, these substances may be combined with cytostatics to enhance cytotoxicity. Here, we investigated the influence of different CDKi´s (palbociclib, dinaciclib, THZ1) on two HNSCC cell lines in monotherapy and combination therapy with clinically-approved drugs (5-FU, Cisplatin, cetuximab). Apoptosis/necrosis, cell cycle, invasiveness, senescence, radiation-induced γ-H2AX DNA double-strand breaks, and effects on the actin filament were studied. Furthermore, the potential to increase tumor immunogenicity was assessed by analyzing Calreticulin translocation and immune relevant surface markers. Finally, an in vivo mouse model was used to analyze the effect of dinaciclib and Cisplatin combination therapy. Dinaciclib, palbociclib, and THZ1 displayed anti-neoplastic activity after low-dose treatment, while the two latter substances slightly enhanced radiosensitivity. Dinaciclib decelerated wound healing, decreased invasiveness, and induced MHC-I, accompanied by high amounts of surface-bound Calreticulin. Numbers of early and late apoptotic cells increased initially (24 h), while necrosis dominated afterward. Antitumoral effects of the selective CDKi palbociclib were weaker, but combinations with 5-FU potentiated effects of the monotherapy. Additionally, CDKi and CDKi/chemotherapy combinations induced MHC I, indicative of enhanced immunogenicity. The in vivo studies revealed a cell line-specific response with best tumor growth control in the combination approach. Global acting CDKi’s should be further investigated as targeting agents for HNSCC, either individually or in combination with selected drugs. The ability of dinaciclib to increase the immunogenicity of tumor cells renders this substance a particularly interesting candidate for immune-based oncological treatment regimens.
The Impact of Time Interval between Hepatic Resection and Liver Transplantation on Clinical Outcome in Patients with Hepatocellular Carcinoma
Authors: Serenari Matteo , Prosperi Enrico , Allard Marc-Antoine , Paterno Michele , Golse Nicolas , Laurenzi Andrea , Adam René , Ravaioli Matteo , Cherqui Daniel , Cescon Matteo
Published: May 2021

Hepatic resection (HR) for hepatocellular carcinoma (HCC) may require secondary liver transplantation (SLT). However, a previous HR is supposed to worsen post-SLT outcomes. Data of patients treated by SLT between 2000 and 2018 at two tertiary referral centers were analyzed. The primary outcome of the study was to analyze the impact of HR on post-LT complications. A Comprehensive Complication Index ≥ 29.6 was chosen as cutoff. The secondary outcome was HCC-related death by means of competing-risk regression analysis. In the study period, 140 patients were included. Patients were transplanted in a median of 23 months after HR (IQR 14–41). Among all the features analyzed regarding the prior HR, only time interval between HR and SLT (time HR-SLT) was an independent predictor of severe complications after LT (OR = 0.98, p < 0.001). According to fractional polynomial regression, the probability of severe complications increased up to 15 months after HR (43%), then slowly decreased over time (OR = 0.88, p < 0.001). There was no significant association between HCC-related death and time HR-SLT at the multivariable competing risks regression model (SHR, 1.06; 95% CI: 0.69–1.62, p = 0.796). This study showed that time HR-SLT was key in predicting complications after LT, without affecting HCC-related death.
Aldo-Keto Reductase 1C3 Mediates Chemotherapy Resistance in Esophageal Adenocarcinoma via ROS Detoxification
Authors: Zhou Chenghui , Wang Zhefang , Li Jiahui , Wu Xiaolin , Fan Ningbo , Li Dai , Liu Fanyu , Plum Patrick S. , Hoppe Sascha , Hillmer Axel M. , Quaas Alexandar , Gebauer Florian , Chon Seung-Hun , Bruns Christiane J. , Zhao Yue
Published: May 2021

Esophageal adenocarcinoma (EAC) is one of the most lethal malignancies, and limits promising treatments. AKR1C3 represents a therapeutic target to combat the resistance in many cancers. However, the molecular mechanism of AKR1C3 in the chemotherapy resistance of EAC is still unclear. We found that the mRNA level of AKR1C3 was higher in EAC tumor tissues, and that high AKR1C3 expression might be associated with poor overall survival of EAC patients. AKR1C3 overexpression decreased cell death induced by chemotherapeutics, while knockdown of AKR1C3 attenuated the effect. Furthermore, we found AKR1C3 was inversely correlated with ROS production. Antioxidant NAC rescued chemotherapy-induced apoptosis in AKR1C3 knockdown cells, while the GSH biosynthesis inhibitor BSO reversed a protective effect of AKR1C3 against chemotherapy. AKT phosphorylation was regulated by AKR1C3 and might be responsible for eliminating over-produced ROS in EAC cells. Intracellular GSH levels were modulated by AKR1C3 and the inhibition of AKT could reduce GSH level in EAC cells. Here, we reported for the first time that AKR1C3 renders chemotherapy resistance through controlling ROS levels via AKT signaling in EAC cells. Targeting AKR1C3 may represent a novel strategy to sensitize EAC cells to conventional chemotherapy.
Development of a Secure Website to Facilitate Information Sharing in Families at High Risk of Bowel Cancer—The Familyweb Study
Authors: Goodman Selina , Skirton Heather , Jackson Leigh , Jones Ray B.
Published: May 2021

Individuals with pathogenic variants in genes predisposing to bowel cancer are encouraged to share this information within their families. Close relatives at 50% risk can have access to bowel cancer surveillance. However, many relatives remain unaware of their vulnerability or have insufficient information. We investigated the feasibility and acceptability of using a secure website to support information sharing within families at high risk of bowel cancer. Patients (n = 286) answered an anonymous cross-sectional survey, with 14 participating in telephone interviews. They reported that the diagnosis had a profound effect on them and their family relationships, and consequently desired more support from health professionals. Website content was created in response to the preferences of survey and interview participants. Reactions to the website from 12 volunteers were captured through remote usability testing to guide further refinement of the website. Participants welcomed the opportunity to store and share personal information via the website and wanted more information and help informing their relatives about the diagnosis. Important website topics were: healthy lifestyle; genetic testing; and how to talk to children about the diagnosis. A website providing online access to confidential documents was both feasible and acceptable and could translate into increased uptake of cancer surveillance, resulting in lower morbidity and mortality in these families.
Impact of Neck Dissection in Head and Neck Squamous Cell Carcinomas of Unknown Primary
Authors: Abu-Shama Yazan , Salleron Julia , Carsuzaa Florent , Sun Xu-Shan , Pflumio Carole , Troussier Idriss , Petit Claire , Caubet Matthieu , Beddok Arnaud , Calugaru Valentin , Servagi-Vernat Stephanie , Castelli Joël , Miroir Jessica , Krengli Marco , Giraud Paul , Romano Edouard , Khalifa Jonathan , Doré Mélanie , Blanchard Nicolas , Coutte Alexandre , Dupin Charles , Sumodhee Shakeel , Tao Yungan , Roth Vincent , Geoffrois Lionel , Toussaint Bruno , Nguyen Duc Trung , Faivre Jean-Christophe , Thariat Juliette
Published: May 2021

Purpose: Management of head and neck cancers of unknown primary (HNCUP) combines neck dissection (ND) and radiotherapy, with or without chemotherapy. The prognostic value of ND has hardly been studied in HNCUP. Methods: A retrospective multicentric study assessed the impact of ND extent (adenectomy, selective ND, radical/radical-modified ND) on nodal relapse, progression-free survival (PFS) or survival, taking into account nodal stage. Results: 53 patients (16.5%) had no ND, 33 (10.2%) had lymphadenectomy, 116 (36.0%) underwent selective ND and 120 underwent radical/radical-modified ND (37.3%), 15 of which received radical ND (4.7%). With a 34-month median follow-up, the 3-year incidence of nodal relapse was 12.5% and progression-free survival (PFS) 69.1%. In multivariate analysis after adjusting for nodal stage, the risk of nodal relapse or progression was reduced with lymphadenectomy, selective or radical/modified ND, but survival rates were similar. Patients undergoing lymphadenectomy or ND had a better PFS and lowered nodal relapse incidence in the N1 + N2a group, but the improvement was not significant for the N2b or N2 + N3c patients. Severe toxicity rates exceeded 40% with radical ND. Conclusion: In HNCUP, ND improves PFS, regardless of nodal stage. The magnitude of the benefit of ND does not appear to depend on ND extent and decreases with a more advanced nodal stage.
STA-MCA Bypass in Carotid Stenosis after Radiosurgery for Cavernous Sinus Meningioma
Authors: Corniola Marco Vincenzo , König Marton , Meling Torstein Ragnar
Published: May 2021

Background: Cavernous sinus meningiomas (CSM) are mostly non-surgical tumors. Stereotactic radiosurgery (SRS) or radiotherapy (SRT) allow tumor control and improvement of pre-existing cranial nerve (CN) deficits. We report the case of a patient with radiation-induced internal carotid artery (ICA) stenosis. We complete the picture with a review of the literature of vascular and non-vascular complications following the treatment of CSMs with SRS or SRT. Methods: After a case description, a systematic literature review is presented, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2015 guidelines. Results: 115 abstracts were screened and 70 titles were retained for full-paper screening. A total of 58 articles did not meet the inclusion criteria. There were 12 articles included in our review, with a follow-up ranging from 33 to 120 months. Two cases of post-SRT ischemic stroke and one case of asymptomatic ICA stenosis were described. Non-vascular complications were reported in all articles. Conclusion: SRS and SRT carry fewer complications than open surgery, with similar rates of tumor control. Our case shows the importance of a follow-up of irradiated CSMs not only by a radio-oncologist, but also by a neurosurgeon, illustrating the importance of multidisciplinary management of CSMs.
Does the Microenvironment Hold the Hidden Key for Functional Precision Medicine in Pancreatic Cancer?
Authors: Kokkinos John , Jensen Anya , Sharbeen George , McCarroll Joshua A. , Goldstein David , Haghighi Koroush S. , Phillips Phoebe A.
Published: May 2021

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers and no significant improvement in patient survival has been seen in the past three decades. Treatment options are limited and selection of chemotherapy in the clinic is usually based on the performance status of a patient rather than the biology of their disease. In recent years, research has attempted to unlock a personalised treatment strategy by identifying actionable molecular targets in tumour cells or using preclinical models to predict the effectiveness of chemotherapy. However, these approaches rely on the biology of PDAC tumour cells only and ignore the importance of the microenvironment and fibrotic stroma. In this review, we highlight the importance of the microenvironment in driving the chemoresistant nature of PDAC and the need for preclinical models to mimic the complex multi-cellular microenvironment of PDAC in the precision medicine pipeline. We discuss the potential for ex vivo whole-tissue culture models to inform precision medicine and their role in developing novel therapeutic strategies that hit both tumour and stromal compartments in PDAC. Thus, we highlight the critical role of the tumour microenvironment that needs to be addressed before a precision medicine program for PDAC can be implemented.
Cryopreservation of Whole Tumor Biopsies from Rectal Cancer Patients Enable Phenotypic and In Vitro Functional Evaluation of Tumor-Infiltrating T Cells
Authors: Liang Frank , Rezapour Azar , Falk Peter , Angenete Eva , Yrlid Ulf
Published: May 2021

TILs comprise functionally distinct conventional and unconventional T cell subsets and their role in responses to CRC treatments is poorly understood. We explored recovery of viable TILs from cryopreserved tumor biopsies of (chemo)-radiated patients with rectal cancer to establish a platform for retrospective TIL analyses of frozen tumors from pre-selected study cohorts. Frequencies of TIL subsets and their capacity to mount IFN-γ responses in cell suspensions of fresh vs. cryopreserved portions of the same tumor biopsies were determined for platform validation. The percentages and proportions of CD4+ TILs and CD8+ cytotoxic T lymphocytes (CTLs) among total TILs were not affected by cryopreservation. While recovery of unconventional γδ T cells and mucosal-associated invariant T cells (MAIT cells) was stable after cryopreservation, the regulatory T cells (Tregs) were reduced, but in sufficient yields for quantification. IFN-γ production by in vitro-stimulated CD4+ TILs, CTLs, γδ T cells, and MAIT cells were proportionally similar in fresh and cryopreserved tumor portions, albeit the latter displayed lower levels. Thus, the proposed platform intended for TIL analyses on cryopreserved tumor biobank biopsies holds promises for studies linking the quantity and quality of TIL subsets with specific clinical outcome after CRC treatment.