bluuebunny/crossref_metadata_2025 huggingface dataset:
Freeze-Dried Immobilized Kefir Culture in Low Alcohol Winemaking
Authors: Nikolaou Anastasios , Sgouros Georgios , Mitropoulou Gregoria , Santarmaki Valentini , Kourkoutas Yiannis
Published: January 2020

Low alcohol wines represent a rising trend in the global market. Since for ethanol removal, certain physicochemical methods that negatively affect wine quality are applied, the aim of this present study was to evaluate the efficiency of freeze-dried, immobilized kefir culture on natural supports (apple pieces, grape skins and delignified cellulosic material) in low alcohol winemaking at various temperatures (5–30 °C). Initially, genetic analysis of kefir culture was performed by Next Generation Sequencing. There was an immobilization of kefir culture on grape skins-enhanced cell survival during freeze-drying in most cases, even when no cryoprotectant was used. Simultaneous alcoholic and malolactic fermentations were performed in repeated batch fermentations for >12 months, using freeze-dried free or immobilized cells produced with no cryoprotectant, suggesting the high operational stability of the systems. Values of great industrial interest for daily ethanol productivity and malic acid conversion [up to 39.5 g/(Ld) and 67.3%, respectively] were recorded. Principal Component Analysis (PCA) showed that freeze-drying rather than the fermentation temperature affected significantly minor volatiles. All low alcohol wines produced were accepted during the preliminary sensory evaluation.
Identification and Characterization of Non-Saccharomyces Species Isolated from Port Wine Spontaneous Fermentations
Authors: Mateus Denisa , Sousa Susana , Coimbra Cláudia , S. Rogerson Frank , Simões João
Published: January 2020

In winemaking, non-Saccharomyces yeast species contribute important organoleptic complexity. Current interest focuses on abundant and dominant strains characteristically present in the early phase of spontaneous alcoholic fermentations. Non-Saccharomyces species are particularly relevant in Port wine production such that the fermentation is prematurely stopped, after the metabolism of only one half of the available sugar, through fortification with aguardente. This work aimed to isolate, identify and characterize non-Saccharomyces species present in spontaneously fermenting Port. To accomplish these goals, yeasts were isolated from a selection of frozen must samples (2012–2016 harvests), using a pre-screening process choosing only the best candidates based on the organoleptic quality of the corresponding fortified wine. From five hundred non-Saccharomyces isolates, twelve species were identified. The three most abundant species, Hanseniaspora uvarum, Lachancea thermotolerans, and Metschnikowia pulcherrima, representing 89% of the isolates, exhibited particularly high diversity with high growth performance variability when exposed to typical stress conditions associated with common enological parameters. Less abundant species included Issatchenkia orientalis, Torulaspora delbrueckii, Hanseniaspora vineae, Hanseniaspora osmophila, Candida zemplinina, Rhodotorula mucilaginosa, Hanseniaspora guilliermondii, Issatchenkia occidentalis, and Zygosaccharomyces bisporus. This is the first study providing insights into the identification and characterization of non-Saccharomyces species responsible for spontaneous Port wine production.
Mineral Composition and Antioxidant Potential of Coffee Beverages Depending on the Brewing Method
Authors: Janda Katarzyna , Jakubczyk Karolina , Baranowska-Bosiacka Irena , Kapczuk Patrycja , Kochman Joanna , Rębacz-Maron Ewa , Gutowska Izabela
Published: January 2020

Coffee, being one of the world’s most popular beverages, is a rich source of dietary antioxidants. The aim of this study was to determine the mineral content and antioxidant activity as well as acidity of coffee beverages depending on the brewing technique. We tested coffee brews made and served at a popular urban coffee shop (Szczecin, Poland). Five coffee brewing techniques were used: Aeropress, drip, espresso machine, French press, and simple infusion. Our findings showed that the brewing method had a significant effect on all parameters tested in the study. The antioxidant activity of the beverages was high (31%–42% inhibition of DPPH (2,2-diphenyl-1-picrylhydrazyl); reduction potential from 3435.06 mol Fe3+/mL to 4298.19 mol Fe3+/mL). Polyphenolic content ranged from 133.90 g (French press) to 191.29 g of gallic acid/L (Aeropress brew), depending on the coffee extraction method. Mineral content was also found to differ between brewing methods. Coffees prepared by simple infusion and Aeropress provided a valuable source of magnesium, manganese, chromium, cobalt, and potassium, whereas the drip brew was found to be a good source of silicon.
Improving Stability and Accessibility of Quercetin in Olive Oil-in-Soy Protein Isolate/Pectin Stabilized O/W Emulsion
Authors: Wang Qiang , Wei Huaheng , Deng Chaofang , Xie Chenjing , Huang Meigui , Zheng Fuping
Published: January 2020

Herein we report a soy protein isolate/pectin binary complex particle to stabilize emulsion (olive oil served as dispersed phase) containing quercetin. FTIR was conducted to confirm successful preparation of emulsion before and after embedding quercetin. CLSM was used to determine the microstructure and zeta-potential, rheological behavior, storage stability and freeze-thaw stability were analyzed and were correlated with pH condition. Olive oil-soy protein isolate/pectin emulsion at pH 3.0 can remain stable after 30 days’ storage and exhibited greatest freeze-thaw stability after 3 cycles. Quercetin availability was evaluated by in vitro gastrointestinal digestion experiments and it reached 15.94% at pH 7.0.
Qishta—A Lebanese Heat Concentrated Dairy Product Characteristics and Production Procedures
Authors: Najib Mustapha , Hallab Mohamad Walid , Hallab Karim , Hallab Zaher , Delaplace Guillaume , Hamze Monzer , Chihib Nour-Eddine
Published: January 2020

This study aims at exploring the chemical composition of a traditional Lebanese dairy product known as Qishta, describing the process of how to prepare it and understanding the mechanisms leading to its formation. The process of making Qishta can be divided into two phases: a hot phase during which milk is heated in a stainless-steel large shallow vessel, and a cold phase consisting of draining, cooling and packaging. According to milk temperature, two reaction zones were identified: zone A with an average temperature of 100 °C, and zone B with an average temperature of 60 °C. The results showed that Qishta had a moisture, fat, protein, lactose and ash content of 68%, 11.7%, 12.1%, 5.4% and 1.6%, respectively. Our findings showed that Qishta is a lipoprotein product having an equal amount of fat and proteins (≈12%); this composition is almost similar to that of Ricotta cheese made from whole milk. In addition, our results assert that the interactions between caseins and whey proteins lead to gel formation. Milk initial fat percentage had a significant effect on Qishta production. The highest yields were obtained when the initial fat percentage was 3.6% (182.5 g of Qishta).
Effect of NOx and NO2 Concentration Increase in Ambient Air to Daily Bronchitis and Asthma Exacerbation, Silesian Voivodeship in Poland
Authors: Kowalska Małgorzata , Skrzypek Michał , Kowalski Michał , Cyrys Josef
Published: January 2020

There is a discussion in Europe about the dominant role of air pollution for health effects, most researchers claim that the particulate matter is responsible for inflammatory processes in the respiratory system, while others underline the role of nitrogen dioxide. The aim of the study was to assess the risk related to NOx, NO2 and PM2.5 concentration increase and daily outpatient visits or hospitalization due to bronchitis and asthma exacerbation in the entire population of Silesian Voivodeship, Poland. To assess the relationship between daily pollutants concentrations and the number of outpatient visits or hospitalizations due to bronchitis and asthma (available in the regional registry), the multivariable log-linear Poisson regression model was used. Results were presented by relative risk (RR) of health outcomes related to the increase in pollutant concentration by unit (interquartile range). Obtained results confirmed a statistically significant association between outpatient visits and hospitalizations due to bronchitis and asthma exacerbation and daily nitrogen oxides concentrations in Silesian voivodeship, Poland. The strongest relationship was observed in the case of NO2 and outpatient visits due to bronchitis, e.g., RR = 1.434 (1.308–1.571) for exposure expressed by the 50-day moving average concentration. In the case of hospitalizations, the health effect was lagged a few days in relation to the increase in exposure.
The SWI/SNF ATP-Dependent Chromatin Remodeling Complex in Arabidopsis Responds to Environmental Changes in Temperature-Dependent Manner
Authors: Gratkowska-Zmuda Dominika M. , Kubala Szymon , Sarnowska Elzbieta , Cwiek Pawel , Oksinska Paulina , Steciuk Jaroslaw , Rolicka Anna T. , Zaborowska Magdalena , Bucior Ernest , Maassen Anna , Franzen Rainer , Koncz Csaba , Sarnowski Tomasz J.
Published: January 2020

SWI/SNF ATP-dependent chromatin remodeling complexes (CRCs) play important roles in the regulation of transcription, cell cycle, DNA replication, repair, and hormone signaling in eukaryotes. The core of SWI/SNF CRCs composed of a SWI2/SNF2 type ATPase, a SNF5 and two of SWI3 subunits is sufficient for execution of nucleosome remodeling in vitro. The Arabidopsis genome encodes four SWI2/SNF2 ATPases, four SWI3, a single SNF5 and two SWP73 subunits. Genes of the core SWI/SNF components have critical but not fully overlapping roles during plant growth, embryogenesis, and sporophyte development. Here we show that the Arabidopsis swi3c mutant exhibits a phenotypic reversion when grown at lower temperature resulting in partial restoration of its embryo, root development and fertility defects. Our data indicates that the swi3c mutation alters the expression of several genes engaged in low temperature responses. The location of SWI3C-containing SWI/SNF CRCs on the ICE1, MYB15 and CBF1 target genes depends on the temperature conditions, and the swi3c mutation thus also influences the transcription of several cold-responsive (COR) genes. These findings, together with genetic analysis of swi3c/ice1 double mutant and enhanced freezing tolerance of swi3c plants illustrate that SWI/SNF CRCs contribute to fine-tuning of plant growth responses to different temperature regimes.
Advanced Methodology and Preliminary Measurements of Molecular and Mechanical Properties of Heart Valves under Dynamic Strain
Authors: Madhurapantula Rama S. , Krell Gabriel , Morfin Berenice , Roy Rajarshi , Lister Kevin , Orgel Joseph P.R.O.
Published: January 2020

Mammalian heart valves are soft tissue assemblies with multi-scale material properties. This is because they are constructs comprising both muscle and non-contractile extracellular matrix proteins (such as collagens and proteoglycans) and transition regions where one form of tissue structure becomes another, significantly different form. The leaflets of the mitral and tricuspid valves are connected to chordae tendinae which, in turn, bind through papillary muscles to the cardiac wall of the ventricle. The transition regions between these tissue subsets are complex and diffuse. Their material composition and mechanical properties have not been previously described with both micro and nanoscopic data recorded simultaneously, as reported here. Annotating the mechanical characteristics of these tissue transitions will be of great value in developing novel implants, improving the state of the surgical simulators and advancing robot-assisted surgery. We present here developments in multi-scale methodology that produce data that can relate mechanical properties to molecular structure using scanning X-ray diffraction. We correlate these data to corresponding tissue level (macro and microscopic) stress and strain, with particular emphasis on the transition regions and present analyses to indicate points of possible failure in these tissues.
Development of Newborn Screening for Pompe Disease
Authors: Hwu Wuh-Liang , Chien Yin-Hsiu
Published: January 2020

Pompe disease is an inborn error of lysosomal degradation of glycogen [...]
Obstructive Sleep Apnea in Neurodegenerative Disorders: Current Evidence in Support of Benefit from Sleep Apnea Treatment
Authors: Lajoie Annie C. , Lafontaine Anne-Louise , Kimoff R. John , Kaminska Marta
Published: January 2020

Obstructive sleep apnea (OSA) is a prevalent disorder characterized by recurrent upper airway obstruction during sleep resulting in intermittent hypoxemia and sleep fragmentation. Research has recently increasingly focused on the impact of OSA on the brain’s structure and function, in particular as this relates to neurodegenerative diseases. This article reviews the links between OSA and neurodegenerative disease, focusing on Parkinson’s disease, including proposed pathogenic mechanisms and current knowledge on the effects of treatment.
Holocene Hurricane Deposits Eroded as Coastal Barriers from Andesite Sea Cliffs at Puerto Escondido (Baja California Sur, Mexico)
Authors: Johnson Markes E. , Johnson Erlend M. , Guardado-France Rigoberto , Ledesma-Vázquez Jorge
Published: January 2020

Previous studies on the role of hurricanes in Mexico’s Gulf of California examined coastal boulder deposits (CBDs) eroded from limestone and rhyolite sea cliffs. Sedimentary and volcanic in origin, these lithotypes are less extensively expressed as rocky shores than others in the overall distribution of gulf shores. Andesite that accumulated as serial volcanic flows during the Miocene constitutes by far the region’s most pervasive rocky shores. Here, we define a subgroup of structures called barrier boulder deposits (BBDs) that close off lagoons as a result of lateral transport from adjacent rocky shores subject to recurrent storm erosion. Hidden Harbor (Puerto Escondido) is the most famous natural harbor in all of Baja California. Accessed from a single narrow entrance, it is commodious in size (2.3 km2) and fully sheltered by outer andesite hills linked by two natural barriers. The average weight of embedded boulders in a succession of six samples tallied over a combined distance of 710 m ranges between 74 and 197 kg calculated on the basis of boulder volume and the specific gravity of andesite. A mathematical formula is utilized to estimate the wave height necessary to transport large boulders from their source. Average wave height interpreted by this method varies between 4.1 and 4.6 m. Input from fossil deposits and physical geology related to fault trends is applied to reconstruct coastal evolution from a more open coastal scenario during the Late Pleistocene 125,000 years ago to lagoon closure in Holocene time.
Long-Term Results of a Second-Generation, Small-Diameter, Metal-On-Metal Bearing in Primary Total Hip Arthroplasty at 14-Year Follow-Up
Authors: Reiner Tobias , Klotz Matthias C. , Seelmann Kirsten , Hertzsch Fabian , Innmann Moritz M. , Streit Marcus R. , Nees Timo A. , Moradi Babak , Merle Christian , Kretzer Jan Philippe , Gotterbarm Tobias
Published: January 2020

(1) Background: The objective of the present study was to review the clinical and radiological results of a small-head, MoM bearing in primary THA and to determine blood metal ion levels at long-term follow-up. (2) Methods: We retrospectively evaluated the clinical and radiological results of 284 small-diameter, MoM 28-mm Metasul THA at a mean follow-up of 14.5 years, and measured blood metal ion concentrations in 174 of these patients. (3) Results: After 14 years, survival free for revision due to any reason was 94%. Proximal femoral osteolysis was seen in 23% of hips, and MRI demonstrated ARMD in 27 of the 66 investigated hips (41%). Mean cobalt, chromium, and titanium ion concentrations were 0.82 µg/L (range 0.22–4.45), 1.51 µg/L (0.04–22.69), and 2.68 µg/L (0.26–19.56) in patients with unilateral THA, and 2.59 µg/L (0.43–24.75), 2.50 µg/L (0.26–16.75), and 3.76 µg/L (0.67–19.77), respectively in patients with bilateral THA. Twenty-nine percent of patients showed cobalt or chromium ion levels > 2 µg/L. (4) Conclusions: Despite good clinical long-term results, increased blood metal ion levels (cobalt or chromium > 2 µg/L) were found in approximately one-third of asymptomatic patients, and proximal femoral osteolysis and ARMD were frequently seen in this cohort. Blood metal ion analysis appears helpful in the long-term follow-up of these patients in order to identify individuals at risk. In accordance with contemporary consensus statements, symptomatic patients with elevated metal ion levels and/or progressive osteolysis should be considered for additional CT or MARS MRI to determine the extent of soft tissue affection prior to revision surgery. Further studies are necessary to investigate the clinical relevance of ARMD in asymptomatic patients with small-head, MoM THA.
Effects of Milling Time, Zirconia Addition, and Storage Environment on the Radiopacity Performance of Mechanically Milled Bi2O3/ZrO2 Composite Powders
Authors: Chen May-Show , Lin Hsiu-Na , Cheng Yu-Chun , Fang Alex , Chen Chin-Yi , Lee Pee-Yew , Lin Chung-Kwei
Published: January 2020

Mineral trioxide aggregate (MTA) typically consists of Portland cement (75 wt.%), bismuth oxide (20 wt.%), and gypsum (5 wt.%) and is commonly used as endodontic cement. Bismuth oxide serving as the radiopacifying material reveals the canal filling effect after clinical treatment. In the present study, bismuth/zirconium oxide composite powder was prepared by high energy ball milling of (Bi2O3)100−x (ZrO2)x (x = 5, 10, 15, and 20 wt.%) powder mixture and used as the radiopacifiers within MTA. The crystalline phases of the as-milled powders were examined by the X-ray diffraction technique. The radiopacities of MTA-like cements prepared by using as-milled composite powders (at various milling stages or different amount of zirconia addition) were examined. In addition, the stability of the as-milled powders stored in an ambient environment, an electronic dry box, or a glove box was investigated. The experimental results show that the as-milled powder exhibited the starting powder phases of Bi2O3 and ZrO2 and the newly formed δ-Bi7.38Zr0.62O2.31 phase. The longer the milling time or the larger the amount of the zirconia addition, the higher the percentage of the δ-Bi7.38Zr0.62O2.31 phase in the composite powder. All the MTA-like cements prepared by the as-milled powder exhibited a radiopacity higher than 4 mmAl that is better than the 3 mmAl ISO standard requirement. The 30 min as-milled (Bi2O3)95(ZrO2)5 composite powder exhibited a radiopacity of 5.82 ± 0.33 mmAl and degraded significantly in the ambient environment. However, storing under an oxygen- and humidity-controlled glove box can prolong a high radiopacity performance. The radiopacity was 5.76 ± 0.08 mmAl after 28 days in a glove box that was statistically the same as the original composite powder.
Effect of Er on Microstructure and Mechanical Properties of 5052 Aluminum Alloy with Big Width-To-Thickness Ratio
Authors: She Xinwei , Jiang Xianquan , Qi Bao , Chen Kang
Published: January 2020

The effect of Er on microstructure and mechanical properties of the 5052 aluminum alloy with a big width-to-thickness ratio was investigated by a metallurgical microscope, scanning electron microscope and tensile testing machine. The results showed that the precipitates were slightly refined after Er addition and Al3Fe was transformed into Al6Fe and AlEr with/without a small amount of Fe or Si. The effect of Er on grain refinement was related to its content. When Er content was lower or higher than 0.4%, the grain would coarsen. Homogenization could refine the grain by controlling Er content and distribution in the Al matrix. Long time homogenization at high temperature would significantly reduce the strength of the 5052 aluminum alloy and 5052 aluminum alloys with low Er content, but help to improve the plasticity of those with high Er content. The ultimate tensile strength, yield strength and elongation of the as-cast 5052 aluminum alloy were 197 MPa, 117 MPa and 22.5% respectively. The strength was the highest, when Er content was 0.4 wt. % and the elongation was the best at 0.1 wt. % Er content.
Bisphosphonate’s Effect on Tongue Mucosa: An Experimental Electron Microscopy Study
Authors: Papamitsou Theodora , Morsi-Yeroyannis Antonios , Papanastasiou Anastasios , Bakalopoulos Nikolaos , Dietrich Eva-Maria , Karachrysafi Sofia , Toskas Alexandros , Mareti Evangelia , Morsi-Yeroyanni Anastasia , Sioga Antonia
Published: January 2020

Background and objectives: Bisphosphonates (BPs) are selective inhibitors of osteoclasts, used for the treatment of bone disorders. The objective of this study is to investigate the possible effects of BPs on the tongue’s mucosa. Materials and Methods: Specimens of the tongue of 20 female 12-month old Wistar rats were taken. Ten were used as control group, while in the remaining alendronate (Fosamax, Merck) was administered per os from 13 weeks. Observation of the harvested samples was made by Transmission Electron Microscopy (TEM). Results: In the experimental group, focal alterations were observed to various extent in all specimens. The basement membrane was intact. Furthermore, an increase at the intercellular space was observed, predominantly at the middle layer, and the desmosomes were disorganized. In the lamina propria focal edema was observed. Conclusions: Investigation on the effect of BPs on the tongue’s mucosa through TEM hasn’t been documented in the past. According to our results, BPs seem to cause mild mucosal lesions on the tongue.
Impedance Measures and a Mounting Technique for Drosophila: Larval Movements, Heart Rate, Imaging, and Electrophysiology
Authors: de Castro Noah , Cooper Robin Lewis
Published: January 2020

Monitoring movements of larval Drosophila with electrical detection allows one to record the behaviors without the use of lights and cameras. This is a suitable technique when studying the use of light-sensitive proteins in optogenetic studies. Electrical measures are feasible to use in determining when a larva starts to move or continues to move after a light induced activation of channelrhodopsin. We have developed a technique using an electrical measure of the media as an index of larval movement. As a proof of concept, recordings with an infrared camera of the larval movement were simultaneous made with electrical measures. The two techniques parallel each other in their ability to index larval movements. Bright light-emitting diode (LED) lights used in optogenetic experiments tend to saturate the detectors of the camera unless filters are used and different filters maybe necessary depending on the LED spectrum and sensitivity of the camera. Impedance measures are independent of the type of LED or brightness. We also assessed the use of a non-solvent based glue (3M Vetbond) to hold larvae in place while measuring synaptic function of neuromuscular junctions, cardiac function and influence of modulators, or activation of light-sensitive channels.
Green Synthesis of Biogenic Silver Nanoparticles for Efficient Catalytic Removal of Harmful Organic Dyes
Authors: David Luminita , Moldovan Bianca
Published: January 2020

The present article reports an environmentally benign method for synthesizing silver nanoparticles using the fruit extract of Viburnum opulus L. as a source of bioactive compounds, which can act as reducing agents of the silver ions and also as stabilizing agents of the obtained nanoparticles. The catalytic ability of the synthesized silver nanoparticles (AgNPs) to remove toxic organic dyes was also evaluated. The biosynthesis of silver nanoparticles was firstly confirmed by UV-Vis spectral analysis, which revealed the presence of the characteristic absorption peak at 415 nm corresponding to the surface plasmon vibration of colloidal silver. Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) studies were conducted to confirm the presence of bioactive phytocompounds, especially phenolics, as capping and stabilizing agents of the AgNPs. The size, morphology and crystalline nature of the synthesized AgNPs were investigated by transmission electron microscopy and X-ray diffraction techniques revealing that the obtained nanoparticles were spherical shaped, with an average diameter of 16 nm, monodispersed, face centered cubic nanoparticles. Further, the catalytic ability in the degradation of tartrazine, carmoisine and brilliant blue FCF dyes by NaBH4 was evaluated. The results demonstrated an efficient activity against all the investigated dyes being an outstanding catalyst for the degradation of brilliant blue FCF. This eco-friendly synthetic approach can generate new tools useful in environmental pollution control.
Surface-Bound Humic Acid Increased Propranolol Sorption on Fe3O4/Attapulgite Magnetic Nanoparticles
Authors: Deng Yuehua , Li Yani
Published: January 2020

This study explored the feasibility of utilizing a novel sorbent humic acid (HA) coated Fe3O4/attapulgite (MATP) magnetic nanoparticles (HMATP) for the sorption of propranolol from aqueous solutions. MATP and bare Fe3O4 nanoparticles were also synthesized under similar preparation conditions. The FTIR, Zeta potential, XRD, VSM, TEM, and TGA analyses were conducted to characterize the sorbent materials. The effects of pH, sorbent dosage, ionic strength, HA in the aqueous solution, contact time and initial sorbate concentration on sorption of propranolol were investigated using batch sorption experiments. The results suggested that the sorption capacity of HMATP showed little change from pH 4 to 10. Na+ and Ca2+ slightly inhibited the sorption of propranolol on HMATP. While HA in solution enhanced both MATP and HMATP, which indicated that HMATP can resist HA interference in water. Further, the less leaching amounts of Fe and HA suggested a good stability of HMATP. In all conditions, sorption capacity of propranolol on HMATP was obviously higher than that on MATP, which indicated that surface-coated HA played an important role in the propranolol sorption process. Electrostatic interaction, cation exchange, hydrogen bonding, and π–π electron donor acceptor interactions were considered as the sorption mechanisms.
Designing a Clinical Pharmacy Primary Care Intervention for Myocardial Infarction Patients Using a Patient and Public Involvement Discussion
Authors: Jalal Zahraa , Paudyal Vibhu , Al-Arkee Shahad , Dyson Gillian , Marriott John
Published: January 2020

Objective: to conduct a Patient and Public Involvement (PPI) focus group session. To help inform the design of a clinical pharmacy intervention in primary care for patients after a coronary event. Methods: this study followed a public involvement method. Community members of the public and community engaged research patients who had experienced myocardial infarction where invited to actively take part in a focus group discussion. This is to share past experiences and provide input and advice into the design of a potential research proposal. The session took place at a cardiac rehabilitation centre. Results: four key themes were identified from the focus group these included: experiences with pharmacy and primary care services, medicines knowledge, the pharmacist role and building rapport with healthcare professionals. Nine participants and three researchers attended the PPI discussion session. Seven of the participants were patients who had experienced a cardiac event in the last three months and two were carers. Primary care pharmacy services both clinical and public health were not very familiar to the participants. Different experiences with clinical pharmacy services were reported by participants, while one experience was reported to be helpful others perceived community pharmacists to be to be busy and isolated behind a counter. A general practice GP based specialist nurse was a familiar model of care unlike a specialist clinical pharmacist GP based care role. Participants reported limited time in GP consultations and the need to book double appointments. Participants stressed the need to receive consistent information about their disease and medication from different professionals involved in their care. Different views were expressed regarding the ability to build rapport with a clinical pharmacist when compared to a GP. Input on study outcomes and design was provided by participants. Conclusion: participants in this session mentioned that a clinical pharmacy intervention after hospital discharge would be useful for their continuity of care. Plans are in place to continue to involve patients and the public in the write up, ethics and dissemination of the potential clinical pharmacy proposal.
Low phytic acid Crops: Observations Based on Four Decades of Research
Authors: Raboy Victor
Published: January 2020

The low phytic acid (lpa), or “low-phytate” seed trait can provide numerous potential benefits to the nutritional quality of foods and feeds and to the sustainability of agricultural production. Major benefits include enhanced phosphorus (P) management contributing to enhanced sustainability in non-ruminant (poultry, swine, and fish) production; reduced environmental impact due to reduced waste P in non-ruminant production; enhanced “global” bioavailability of minerals (iron, zinc, calcium, magnesium) for both humans and non-ruminant animals; enhancement of animal health, productivity and the quality of animal products; development of “low seed total P” crops which also can enhance management of P in agricultural production and contribute to its sustainability. Evaluations of this trait by industry and by advocates of biofortification via breeding for enhanced mineral density have been too short term and too narrowly focused. Arguments against breeding for the low-phytate trait overstate the negatives such as potentially reduced yields and field performance or possible reductions in phytic acid’s health benefits. Progress in breeding or genetically-engineering high-yielding stress-tolerant low-phytate crops continues. Perhaps due to the potential benefits of the low-phytate trait, the challenge of developing high-yielding, stress-tolerant low-phytate crops has become something of a holy grail for crop genetic engineering. While there are widely available and efficacious alternative approaches to deal with the problems posed by seed-derived dietary phytic acid, such as use of the enzyme phytase as a feed additive, or biofortification breeding, if there were an interest in developing low-phytate crops with good field performance and good seed quality, it could be accomplished given adequate time and support. Even with a moderate reduction in yield, in light of the numerous benefits of low-phytate types as human foods or animal feeds, should one not grow a nutritionally-enhanced crop variant that perhaps has 5% to 10% less yield than a standard variant but one that is substantially more nutritious? Such crops would be a benefit to human nutrition especially in populations at risk for iron and zinc deficiency, and a benefit to the sustainability of agricultural production.
Parametric Dimensional Analysis on a C-H2 Smelting Reduction Furnace with Double-Row Side Nozzles
Authors: Xie Jinyin , Wang Bo , Zhang Jieyu
Published: January 2020

Higher requirements for steel smelting technology have been put forward based on the increasing awareness of energy conservation and environmental protection. In the field of iron making, carbon reduction processes are often used. In this study, molten iron was smelted by designing a C-H2 smelting reduction method. Although previous researchers have studied this through a large number of physical and numerical simulations, they have not yet refined general laws from the perspective of dimensional analysis. In this paper, a double-row side blow hydraulics simulation was carried out in the C-H2 smelting reduction furnace, and an entire list of dimensionless groups of input and output parameters was proposed based on its hydraulics simulation data. The expressions between the dimensionless group of mixing time and dimensionless groups such as Capillary number (Ca) and Lagrange group (La1) were obtained by multiple linear regression based on the experimental research results and data analysis. By verifying the calculated and experimental values of the dimensionless group of mixing time, it can be seen that both have a good positive correlation. This study provides a better methodology for controlling key parameters and lays the foundation for the optimal design of the process parameters for the C-H2 smelting reduction furnace.
The Use of Artificial Neural Networks for Forecasting of Air Temperature inside a Heated Foil Tunnel
Authors: Francik Sławomir , Kurpaska Sławomir
Published: January 2020

It is important to correctly predict the microclimate of a greenhouse for control and crop management purposes. Accurately forecasting temperatures in greenhouses has been a focus of research because internal temperature is one of the most important factors influencing crop growth. Artificial Neural Networks (ANNs) are a powerful tool for making forecasts. The purpose of our research was elaboration of a model that would allow to forecast changes in temperatures inside the heated foil tunnel using ANNs. Experimental research has been carried out in a heated foil tunnel situated on the property of the Agricultural University of Krakow. Obtained results have served as data for ANNs. Conducted research confirmed the usefulness of ANNs as tools for making internal temperature forecasts. From all tested networks, the best is the three-layer Perceptron type network with 10 neurons in the hidden layer. This network has 40 inputs and one output (the forecasted internal temperature). As the networks input previous historical internal temperature, external temperature, sun radiation intensity, wind speed and the hour of making a forecast were used. These ANNs had the lowest Root Mean Square Error (RMSE) value for the testing data set (RMSE value = 3.7 °C).
A Weigh-in-Motion Characterization Algorithm for Smart Pavements Based on Conductive Cementitious Materials
Authors: Birgin Hasan Borke , Laflamme Simon , D’Alessandro Antonella , Garcia-Macias Enrique , Ubertini Filippo
Published: January 2020

Smart materials are promising technologies for reducing the instrumentation cost required to continuously monitor road infrastructures, by transforming roadways into multifunctional elements capable of self-sensing. This study investigates a novel algorithm empowering smart pavements with weigh-in-motion (WIM) characterization capabilities. The application domain of interest is a cementitious-based smart pavement installed on a bridge over separate sections. Each section transduces axial strain provoked by the passage of a vehicle into a measurable change in electrical resistance arising from the piezoresistive effect of the smart material. The WIM characterization algorithm is as follows. First, basis signals from axles are generated from a finite element model of the structure equipped with the smart pavement and subjected to given vehicle loads. Second, the measured signal is matched by finding the number and weights of appropriate basis signals that would minimize the error between the numerical and measured signals, yielding information on the vehicle’s number of axles and weight per axle, therefore enabling vehicle classification capabilities. Third, the temporal correlation of the measured signals are compared across smart pavement sections to determine the vehicle weight. The proposed algorithm is validated numerically using three types of trucks defined by the Eurocodes. Results demonstrate the capability of the algorithm at conducting WIM characterization, even when two different trucks are driving in different directions across the same pavement sections. Then, a noise study is conducted, and the results conclude that a given smart pavement section operating with less than 5% noise on measurements could yield good WIM characterization results.
Design, Development and Implementation of the Position Estimator Algorithm for Harmonic Motion on the XY Flexural Mechanism for High Precision Positioning
Authors: Shewale Mahesh , Razban Ali , Deshmukh Suhas , Mulik Sharad
Published: January 2020

This article presents a novel concept of the position estimator algorithm for voice coil actuators used in precision scanning applications. Here, a voice coil motor was used as an actuator and a sensor using the position estimator algorithm, which was derived from an electro-mechanical model of a voice coil motor. According to the proposed algorithm, the position of coil relative to the fixed magnet position depends on the current drawn, voltage across coil and motor constant of the voice coil motor. This eliminates the use of a sensor that is an integral part of all feedback control systems. Proposed position estimator was experimentally validated for the voice coil actuator in integration with electro-mechanical modeling of the flexural mechanism. The experimental setup consisted of the flexural mechanism, voice coil actuator, current and voltage monitoring circuitry and its interfacing with PC via a dSPACE DS1104 R&D microcontroller board. Theoretical and experimental results revealed successful implementation of the proposed novel algorithm in the feedback control system with positioning resolution of less than ±5 microns at the scanning speed of more than 5 mm/s. Further, proportional-integral-derivative (PID) control strategy was implemented along with developed algorithm to minimize the error. The position determined by the position estimator algorithm has an accuracy of 99.4% for single direction motion with the experimentally observed position at those instantaneous states.
The Rehapiano—Detecting, Measuring, and Analyzing Action Tremor Using Strain Gauges
Authors: Ferenčík Norbert , Jaščur Miroslav , Bundzel Marek , Cavallo Filippo
Published: January 2020

We have developed a device, the Rehapiano, for the fast and quantitative assessment of action tremor. It uses strain gauges to measure force exerted by individual fingers. This article verifies the device’s capability to measure and monitor the development of upper limb tremor. The Rehapiano uses a precision, 24-bit, analog-to-digital converter and an Arduino microcomputer to transfer raw data via a USB interface to a computer for processing, database storage, and evaluation. First, our experiments validated the device by measuring simulated tremors with known frequencies. Second, we created a measurement protocol, which we used to measure and compare healthy patients and patients with Parkinson’s disease. Finally, we evaluated the repeatability of a quantitative assessment. We verified our hypothesis that the Rehapiano is able to detect force changes, and our experimental results confirmed that our system is capable of measuring action tremor. The Rehapiano is also sensitive enough to enable the quantification of Parkinsonian tremors.
An Ensemble Framework to Investigate Wind Energy Sustainability Considering Climate Change Impacts
Authors: Wang Shengjin , Yang Hongru , Pham Quoc Bao , Khoi Dao Nguyen , Nhi Pham Thi Thao
Published: January 2020

Wind power is a key element for future renewable energy resources and plays a vital role in sustainable development. Global warming and future climate conditions are going to impact many atmospheric, oceanic, and earth systems. In this study, impacts of climate change on wind power resources under future climatic conditions are evaluated for the Persian Gulf to explore the sustainability of this kind of energy for present and future developments. To that end, three regional climate models obtained from coordinated regional downscaling experiment (CRODEX), including daily simulations of near-surface wind speeds for a 20-year period in the present and future, were considered. Prior to computing the wind power at turbine hub-height, historical simulations of CORDEX were evaluated versus ERA-Interim wind outputs to determine the accuracy of the regional climate models. An attempt was made to build an ensemble model from available models by assigning weights to the models based on their merits. Subsequently, the wind power at the turbine hub-height was computed for historical and future periods to detect the impacts of climate change. Some points with a relatively high energy potential were selected as energy hotspots for further investigations. The results revealed that the mean annual wind power over the study area changed remarkably, which is of great importance for sustainable developments. Moreover, the results of the directional investigations showed roughly the same directional distribution for the future period as the past.
Frame Disputes or Frame Consensus? “Environment” or “Welfare” First Amongst Climate Strike Protesters
Authors: Emilsson Kajsa , Johansson Håkan , Wennerhag Magnus
Published: January 2020

Present debates suppose a close linkage between economic, social, and environmental sustainability and suggest that individual wellbeing and living standards need to be understood as directly linked to environmental concerns. Because social movements are often seen as an avant-garde in pushing for change, this article analyzes climate protesters’ support for three key frames in current periods of social transformation, i.e., an “environmental”, an “economic growth”, and a “welfare” frame. The analyzed data material consists of survey responses from over 900 participants in six Global Climate Strikes held in Sweden during 2019. The article investigates the explanatory relevance of three factors: (a) political and ideological orientation, (b) movement involvement, and (c) social characteristics. The results indicate that climate protesters to a large degree support an environmental frame before an economic growth-oriented frame, whereas the situation is more complex regarding support for a welfare frame vis-á-vis an environmental frame. The strongest factors explaining frame support include social characteristics (gender) and protestors’ political and ideological orientation. Movement involvement has limited significance. The article shows how these frames form a fragment of the complexity of these issues, and instances of frame distinctions, hierarchies, and disputes emerge within the most current forms of climate change demonstrations.
Analysis of Dissolved Oxygen and Nutrients in Zhanjiang Bay and the Adjacent Sea Area in Spring
Authors: Fu Dongyang , Zhong Yafeng , Chen Fajin , Yu Guo , Zhang Xiaolong
Published: January 2020

As a semi-closed bay with narrow bay mouths, the distribution of nutrients in Zhanjiang Bay was different from bays with open bay mouths and rivers with large flows. It is important to study the water quality of Zhanjiang Bay to determine the impact of human activities on this semi-closed bay. Based on field survey data in spring, the spatial distribution of nutrients and other physico-chemical parameters was investigated, in order to study the geochemical characteristics of nutrients in semi-closed bays. Higher nutrient concentrations were observed in the inner and outer bays, but lower concentrations were observed at the bay mouth. With other analyses of physico-chemical parameters, the higher nutrient concentrations in the inner bay originated mainly from the diluted freshwater input from local developments and rivers. With the strong flow that exists along the western coast of Guangdong Province, the higher dissolved inorganic nitrogen (DIN) and SiO3–Si concentrations along the outer bay may be influenced by discharge from local cities in western Guangdong Province. There was stronger phytoplankton assimilation at the bay mouth, which resulted in reduced nutrient concentrations in this area. Although the hydrographic characteristics between the inner bay and outer bay were significantly different, the distribution of chlorophyll-a (Chla) levels was similar. However, we found significantly low dissolved oxygen (DO) and high apparent oxygen utilisation (AOU) consumption levels in the inner bay, and high DO and low AOU levels in the outer bay, which suggested that decomposition was more important than photosynthesis in the closed bay, even in spring during the phytoplankton bloom.
Willingness to Pay for Forest Existence Value and Sustainability
Authors: Bamwesigye Dastan , Hlavackova Petra , Sujova Andrea , Fialova Jitka , Kupec Petr
Published: January 2020

Uganda is richly endowed with flora and fauna. Until the early 2000s, most of the types of vegetation have remained natural/virgin forests and shrubs until recent years, when human activities have damaged them. Understanding the different ways that people value such endangered forest resources is very important. The main hypothesis in our study is that willingness to pay (WTP) for forest existence value and sustainability depends on the preference for the same values. In addition, we examined socioeconomic characteristics, such as sex, education, and household incomes, which could influence the WTP for forest existence value and sustainability. We carried out field questionnaire interviews with the aim of ascertaining Willingness to Pay (WTP) for forest existence. The WTP values were in a range between 1 and 200 USD based on the contingent valuation method (CVM). A sample with a size of 203 was interviewed in selected towns and villages in Uganda, and the data collected were subjected to statistical analysis. The cross-tabulation of the expressed preferences illustrates that 81.9% of the representative sample are willing to pay for forest existence value and sustainability. We concluded that the willingness to pay for forest existence significantly depends on the preference for forest existence values and sustainability. Our results equally express that the mean WTP in this region is 15 USD per year and that over 60% are willing to pay this amount. The socioeconomic determinants’ results demonstrate heterogeneity and that over 90% of the respondents are willing to pay for forest existence, conservation, and sustainability.
Black Hole Evaporation: A Perspective from Loop Quantum Gravity
Authors: Ashtekar Abhay
Published: January 2020

A personal perspective on the black hole evaporation process is presented using, as guidelines, inputs from: (i) loop quantum gravity, (ii) simplified models where concrete results have been obtained, and, (iii) semi-classical quantum general relativity. On the one hand, the final picture is conservative in that there are concrete results that support each stage of the argument, and there are no large departures from general relativity or semi-classical gravity in tame regions outside macroscopic black holes. On the other hand, it argues against certain views that are commonly held in many quarters, such as persistence of a piece of singularity that constitutes a part of the final boundary of space–time; presence of an event horizon serving as an absolute barrier between the interior and the exterior, and the (often implicit) requirement that purification must be completed by the time the ‘last rays’ representing the extension of this event horizon reach I + .