bluuebunny/crossref_metadata_2025 huggingface dataset:
New Insights into the Depressive Mechanism of Sodium Silicate on Bastnaesite, Parisite, and Fluorite: Experimental and DFT Study
Authors: Wang Jieliang , Lu Wenda , Cao Zhao , Wu Xu , Wang Peng , Wang Xiaoping , Liu Wenli
Published: August 2024

The surface properties of bastnaesite and parisite are similar to their associated gangue mineral, fluorite, which makes the flotation separation of these two rare earth minerals from fluorite one of the industry’s most significant challenges. This study systematically investigates the inhibitory effects and mechanisms of sodium silicate (SS) on bastnaesite, parisite, and fluorite in an octyl hydroxamic acid (OHA) collector system through flotation experiments, various modern analytical methods, and DFT simulations. The flotation test results indicate that the inhibition effects of SS on the three minerals are in the order: fluorite > parisite > bastnaesite. Detection and analysis results indicate that SS forms hydrophilic complexes with Ca atoms on the surfaces of fluorite and parisite, enhancing surface hydrophilicity and inhibiting OHA adsorption, but its impact on bastnaesite is relatively minor. DFT simulation results show that OHA forms covalent bonds with metal ions on mineral surfaces, favoring five-membered hydroxamic-(O-O)-Ce/Ca complexes, and reacts more strongly with Ce atoms than Ca atoms. SS primarily forms covalent bonds with metal atoms on mineral surfaces via the SiO(OH)3− component, and OHA and SS compete for adsorption on the mineral surfaces. OHA has a stronger affinity for bastnaesite, whereas SS shows the highest affinity for fluorite, followed by parisite, and the weakest affinity for bastnaesite.
Exploring the Impact of Astaxanthin Supplementation in Conjunction with a 12-Week CrossFit Training Regimen on Selected Adipo-Myokines Levels in Obese Males
Authors: Moqaddam Mohammad Ahmadi , Nemati Morteza , Dara Marjan Mansouri , Hoteit Maha , Sadek Zahra , Ramezani Akbar , Rand Mahboubeh Khak , Abbassi-Daloii Asieh , Pashaei Zhaleh , Almaqhawi Abdullah , Razi Omid , Escobar Kurt A. , Supriya Rashmi , Saeidi Ayoub , Zouhal Hassane
Published: August 2024

Objective: Obesity is associated with an exacerbated metabolic condition that is mediated through impairing balance in the secretion of some adipo-myokines. Therefore, the objective of the present study was to explore the impact of astaxanthin supplementation in conjunction with a 12-week CrossFit training regimen on some selected adipo-myokines, insulin insensitivity, and serum lipid levels in obese males. Material and Methods: This study is a randomized control trial design; 60 obese males were randomly divided into four groups of 15, including the control group (CG), supplement group (SG), training group (TG), and combined training and supplement group (TSG). The participants were subjected to 12 weeks of astaxanthin (AST) supplementation [20 mg/d capsule, once/d] or CrossFit training or a combination of both interventions. The training regimen comprised 36 sessions of CrossFit, each lasting 60 min, conducted three times per week. The metabolic indices, body composition, anthropometrical, cardio-respiratory, and also some plasma adipo-myokine factors, including decorin (DCN), activin A, myostatin (MST), transforming growth factor (TGF)-β1, and follistatin (FST), were examined 12 and 72 h before the initiation of the main interventional protocols, and then 72 h after the final session of the training protocol. Results: There was no significant difference in the baseline data between the groups (p > 0.05). There were significant interactions between group x time for DCN (η2 = 0.82), activin A (η2 = 0.50), FST (η2 = 0.92), MST (η2 = 0.75), and TGFB-1 (η2 = 0.67) (p < 0.001 for all the variables). Significantly changes showed for DCN in TSG compared to TG and SG and also TG compared to SG (p = 0.0001); for activin A in SG compared to TG (p = 0.01) and TSG (p = 0.002); for FST in SG compared to TG and TSG (p = 0.0001), also in TSG compared to TG (p = 0.0001); for MST in SG, TG, and TSG compared to CG (p = 0.0001) and also in TSG compared to SG (p = 0.0001) and TG (p = 0.001); for TGFB-1 in SG, TG, and TSG compared to CG (p = 0.0001) and also TSG compared to SG (p = 0.0001) and TG (p = 0.001). Conclusions: The 12-week CrossFit training concurrent with AST supplementation reduced anthropometric and metabolic factors and also serum lipid levels while producing positive changes in body composition and cardiovascular factors. Increased FST and DCN and reduced activin A, MST, and TGF-β1 were other affirmative responses to both interventions.
Human Milk Oligosaccharides in Combination with Galacto- and Long-Chain Fructo-Oligosaccharides Enhance Vaccination Efficacy in a Murine Influenza Vaccination Model
Authors: Azarmi Mehrdad , Seyed Toutounchi Negisa , Hogenkamp Astrid , Thijssen Suzan , Overbeek Saskia A. , Garssen Johan , Folkerts Gert , van’t Land Belinda , Braber Saskia
Published: August 2024

Early-life nutrition significantly impacts vaccination efficacy in infants, whose immune response to vaccines is weaker compared to adults. This study investigated vaccination efficacy in female C57Bl/6JOlaHsd mice (6 weeks old) fed diets with 0.7% galacto-oligosaccharides (GOS)/long-chain fructo-oligosaccharides (lcFOS) (9:1), 0.3% human milk oligosaccharides (HMOS), or a combination (GFH) for 14 days prior to and during vaccination. Delayed-type hypersensitivity (DTH) was measured by assessing ear swelling following an intradermal challenge. Influvac-specific IgG1 and IgG2a levels were assessed using ELISAs, while splenic T and B lymphocytes were analyzed for frequency and activation via flow cytometry. Additionally, cytokine production was evaluated using murine splenocytes co-cultured with influenza-loaded dendritic cells. Mice on the GFH diet showed a significantly enhanced DTH response (p < 0.05), increased serological IgG1 levels, and a significant rise in memory B lymphocytes (CD27+ B220+ CD19+). GFH-fed mice also exhibited more activated splenic Th1 cells (CD69+ CXCR3+ CD4+) and higher IFN-γ production after ex vivo restimulation (p < 0.05). These findings suggest that GOS/lcFOS and HMOS, particularly in combination, enhance vaccine responses by improving memory B cells, IgG production, and Th1 cell activation, supporting the potential use of these prebiotics in infant formula for better early-life immune development.
Locally Prepared Therapeutic Food for Treatment of Severely Underweight Children in Rural India: An Interventional Prospective Controlled Community-Based Study with Long Follow-Up:—‘SAMMAN’ Trial
Authors: Satav Ashish Rambhau , Dani Vibhawari S. , Pendharkar Jayashri S. , Satav Kavita Ashish , Raje Dhananjay , Jain Dipty , Khirwadkar Shubhada S. , Simões Eric A. F.
Published: August 2024

Background: Severely underweight (SUW) children contribute significantly to under-five mortality and morbidity. There are WHO guidelines for the management of severe acute malnutrition but no specific guidelines for SUW management. Objective: The objectives were to achieve a recovery rate of 30% at 90 days of treatment for severe underweight (SUW) children aged 6–60 months, compare changes in weight-for-age Z (WAZ) scores, growth patterns, and case fatality rates between intervention and reference arms (RA), and reduce the prevalence of SUW in the intervention arm (IA). The target of a 30% recovery rate was achievable and significant based on our past research conducted in similar settings. Methods: Design: A prospective controlled community-based, longitudinal, two arms (IA, RA), intervention study with long follow-up was conducted between January 2011 and October 2023. Setting: Primary care for participants from 14 villages in rural Melghat, India. Participants: The study participants included SUW children aged 6–60 months and age-matched (±2 weeks) normal controls. The SAMMAN (Acronym for SAM-Management) intervention was comprised of local therapeutic food-micronutrient (LTF-MN) therapy for 90 days, intensive behavior change communication, infection treatment, and quarterly anthropometric records. SUW recovery, growth patterns, case fatality rate, prevalence at 90 days of therapy and at 60 months of age, and survival until early adolescence were assessed. ANCOVA analysis was used to obtain changes in Z-scores. Results: In the IA, the recovery rate was 36.8% at 90 days and 78.2% at 60 months of age. The mean difference in change in WAZ scores between the intervention arm and the reference arm was statistically significant (p < 0.0001). Growth patterns were similar between the two arms up to early adolescence. The SUW case fatality rate was significantly lower in the IA (0.9%) as compared to 4.62% in the RA at 60 months (p = 0.022). The reduction in SUW prevalence in intervention villages was higher than in the control villages (p < 0.001). The cost of management per SUW child was 3888 INR (47 USD) less than RUTF. Conclusion: The SAMMAN intervention is safe and cost-effective for significantly improving WAZ scores, sustainable, and hence replicable in resource-limited areas.
The Moderating Role of Dietary Quality and Dietary Fibre Intake on the Mood Effects of Positive Expressive Writing: A Pilot Study
Authors: Levová Lucie , Smith Michael A.
Published: August 2024

Background: Positive expressive writing is associated with enhanced psychological wellbeing. Several individual differences are known to moderate the enhancement effects of positive expressive writing, but no studies to date have investigated the optimal dietary conditions under which expressive writing effects occur. In this pilot study, we sought to investigate whether diet quality and dietary fibre intake moderate the effects of positive writing on mood. Methods: The participants (12 males, 25 females, Mage = 33.0, SDage = 13.1) completed self-reported measures of dietary quality, dietary fibre intake, and positive and negative affect. They were then randomly allocated to complete either a positive expressive writing or neutral writing activity for 10 min. Positive and negative affect were measured again immediately after each activity. Results: Those participants who reported better diet quality and greater dietary fibre intake exhibited a significantly greater increase in positive affect following positive expressive writing relative to neutral writing. No significant effects were observed for negative affect. Conclusions: For the first time, we report that the effects of positive expressive writing on positive mood are enhanced under optimal dietary conditions. Further replication studies are needed to determine whether dietary factors can influence the conditions under which positive expressive writing benefits occur. We speculate that dietary influences on the gut–brain axis are a potential mechanism.
Optimized Continuity of Care Report on Nursing Compliance and Review: A Retrospective Study
Authors: Luna-Aleixos David , Francisco-Montesó Lorena , López-Negre Marta , Blasco-Peris Débora , Llagostera-Reverter Irene , Valero-Chillerón María Jesús , Cervera-Pitarch Ana Dolores , Gallego-Clemente Andreu , Leal-Costa César , González-Chordá Víctor M.
Published: August 2024

The Continuity of Care Report (CCR) is a fundamental document for ensuring high-quality healthcare and a smooth transition between different levels of care. The aim of this study was to evaluate the impact of optimizing the CCR to improve its completion rate by hospital nurses and its review by primary care nurses. To achieve this, a retrospective observational study was conducted on patients discharged from the University Hospital of La Plana de Vila-real during two three-month periods, one prior to the CCR improvement (2022) and one after (2023). No increase in the completion rate for the CCR was observed following its optimization (p = 0.226). However, a statistically significant improvement was noted in the percentage of reports reviewed (p > 0.001), increasing from 4.4% (n = 49) in 2022 to 30.5% (n = 327) in 2023. These results indicate that the optimization of the Continuity of Care Report enhances the communication between specialized care and primary care professionals.
Determining Factors in the Implementation of Biosecurity Measures by Hospital Nurses in Piura, Peru
Authors: Agurto Córdova Luz Mirella , Espino Carrasco Danicsa Karina , Santa Cruz Espino Briseidy Massiel , Espino Carrasco Mayury , Vargas Cabrera Cindy , Vásquez Cachay Royer , Dávila Valdera Lady , Valdera Benavides Edson David , Valderrama Soto Roque
Published: August 2024

Nosocomial infections are a significant cause of morbidity, mortality, and increased treatment costs in hospitals. This study aimed to analyze the factors determining the implementation of biosafety measures by the nursing staff of four hospitals in Piura via a structural equation modeling (SEM) approach. A total of 215 nurses from various hospitals in the region participated by completing an online survey. The results demonstrated that extrinsic factors (FEX) positively influence the implementation of biosafety measures (BIOM) (β = 0.319 ***), as do intrinsic factors (FINT) (β = 0.520 **). Furthermore, intrinsic factors mediate the relationship between extrinsic factors and the implementation of biosafety measures (β = 0.443 ***). In conclusion, this study provides a deeper understanding of biosafety dynamics in healthcare settings and lays the groundwork for the development of customized interventions and ongoing training programs that ensure the optimal implementation of biosafety measures in hospitals.
Dense Water Formation Variability in the Aegean Sea from 1947 to 2023
Authors: Potiris Manos , Mamoutos Ioannis G. , Tragou Elina , Zervakis Vassilis , Kassis Dimitris , Ballas Dionysios
Published: August 2024

The formation of dense water in the Aegean Sea is important as it affects the deep circulation and the hydrography of the Eastern Mediterranean Sea. In this study, the variability of dense water formation is investigated in relation to forcing mechanisms from 1947 to 2023 in the subbasins of the Aegean Sea, utilising in situ observations from various sources, which have been analysed in combination with satellite altimetry and reanalyses products. The analysis reveals that the Aegean Sea has been in a state of increased dense water formation since 2017 due to the combination of increased surface buoyancy loss and reduced Black Sea water inflow. Extremely high salinity has been recorded in the intermediate layers of the Aegean Sea since 2019. The anticyclonic circulation of the North Ionian gyre during 2017 and 2018 probably also contributed to the rapid transport of highly saline waters in the intermediate and, through dense water formation, the deep layers of the Aegean Sea in 2019. Until 2022, the dense waters formed during the peak of the Eastern Mediterranean Transient still occupied the bottom layers of some deep subbasins of the North and South Aegean; however, the 29.4 kg m−3 isopycnal in the North Aegean and the 29.3 kg m−3 isopycnal in the Southeastern Aegean have gradually deepened by 800 m, permitting the waters forming in the last ten years in the Aegean Sea to settle at ever greater depths. Temperature controls the density variability of the Cretan intermediate water up to the decadal time scale. Increased data availability since 2010 was sufficient to clarify that intrusions of dense water from the North–Central Aegean Sea contributed to the erosion of the Eastern Mediterranean transitional waters in the South Aegean Sea after 2017, as well as to raising the intermediate water masses of the South Aegean to shallower depths. The erosion of the transitional Mediterranean waters in the South Aegean Sea between 1947 and 1955 and 1973 and 1980 coincided with increased dense water formation in the North–Central Aegean Sea. During the peak of the Eastern Mediterranean Transient, the North Ionian circulation, the Black Sea water inflow, the Atlantic Multidecadal Oscillation, and the surface buoyancy fluxes favoured dense water formation in the Aegean Sea.
Detection of Salmonella Mbandaka Carrying the blaCTX-M-8 Gene Located on IncI1 Plasmid Isolated from a Broiler Flock Environment
Authors: Zając Magdalena , Skarżyńska Magdalena , Lalak Anna , Iwan Ewelina , Wasyl Dariusz
Published: August 2024

Salmonella Mbandaka is one of the most globally widespread serovars, occurring in many sources and included among twenty serovars that contribute to human salmonellosis in Europe. In Poland, it has been noted in non-human sources since 1996, being found firstly in feeds and later in waterfowl and chicken. Over the years, it gained epidemiological importance, being isolated from a wide range of animal species, including livestock. Generally, it is characterized by sensitivity to most antimicrobials and the ability to form biofilms. The occurrence of cephalosporin-resistant Salmonella in non-human sources is an extremely rare phenomenon in Poland. In this report, we characterized the full genome of the ESBL-producing S. Mbandaka strain isolated from a broiler farm environment (boot swab sample) in Poland in 2022. The isolate was serotyped as S. Mbandaka according to the White–Kaufmann–Le Minor scheme. Antimicrobial susceptibility testing performed with the microbroth dilution method showed its resistance to ampicillin, cefotaxime, ceftazidime, ciprofloxacin, and nalidixic acid. The whole-genome sequence was reconstructed using short and long reads and assembled into the complete chromosome and three plasmids: IncI1 pST113 (89,439 bp), Col(pHAD28) (2699 bp), and Col440 (2495 bp). The strain belonged to sequence type ST413. Plasmid analysis showed blaCTX-M-8 mobilization on IncI1(alpha) surrounded with insertion sequences. The analyzed genome content draws attention to the possibility of the horizontal spread of the resistance genes. To the best of our knowledge, this is the first report of blaCTX-M-8-positive Salmonella in Poland.
Correction: El-Shesheny et al. Highly Pathogenic Avian Influenza A(H5N1) Virus Clade 2.3.4.4b in Wild Birds and Live Bird Markets, Egypt. Pathogens 2023, 12, 36
Authors: El-Shesheny Rabeh , Moatasim Yassmin , Mahmoud Sara H. , Song Yi , El Taweel Ahmed , Gomaa Mokhtar , Kamel Mina Nabil , Sayes Mohamed El , Kandeil Ahmed , Lam Tommy T. Y. , McKenzie Pamela P. , Webby Richard J. , Kayali Ghazi , Ali Mohamed Ahmed
Published: August 2024

Text Correction [...]
The Stability Optimization of Indoor Visible 3D Positioning Algorithms Based on Single-Light Imaging Using Attention Mechanism Convolutional Neural Networks
Authors: Ji Wenjie , Hu Lianxin , Zhang Xun , Lou Jiongnan , Chen Hongda , Wang Zefeng
Published: August 2024

In recent years, visible light positioning (VLP) techniques have been gaining popularity in research. Among them, the scheme of using a camera as a receiver provides a low-cost, high-precision positioning capability and easy integration with existing multimedia devices and robots. However, the pose changes of the receiver can lead to image distortion and light displacement, significantly increasing positioning errors. Addressing these errors is crucial for enhancing the accuracy of VLP. Most current solutions rely on gyroscopes or Inertial Measurement Units (IMUs) for error optimization, but these approaches often add complexity and cost to the system. To overcome these limitations, we propose a 3D positioning algorithm based on an attention mechanism convolutional neural network (CNN) aimed at reducing the errors caused by angles. We designed experiments and comparisons within a rotation angle range of ±15 degrees. The results demonstrate that the average error for 2D positioning is within 5.74 cm and the height error is within 3.92 cm, and the average error for 3D positioning is within 6.8 cm. Among the four groups of experiments for 3D positioning, compared to the traditional algorithm, the improvements were 7.931 cm, 15.569 cm, 6.004 cm, and 16.506 cm. The experiments indicate that it can be applied to high-precision visible light positioning for single-light imaging.
Integrated Analysis of Metabolomics, Flavoromics, and Transcriptomics for Evaluating New Varieties of Amomum villosum Lour.
Authors: Li Zhenkai , Luo Xin , Yao Yanli , Wang Yukun , Dai Zhiheng , Cheng Tianle , Huang Xinzhi , Bai Mei , He Junjun , Wu Hong
Published: August 2024

Amomum villosum Lour. (A. villosum) is the original plant of the medicinal and culinary spice Amomi Fructus (Sharen) and is an important economic crop in the Lingnan region of China. During the cultivation and production of A. villosum, prolonged reliance on single asexual reproduction has exacerbated the degradation of its varieties, leading to inconsistent yields and quality. Building upon earlier cultivar selection efforts, this study provides a comprehensive evaluation of two newly bred A. villosum varieties (A11 and A12) from perspectives including plant traits, product characteristics, active ingredients, and multi-omics analysis. It was found that A12 plants display enhanced robustness, more aromatic fruits, higher yields, and elevated levels of bornyl acetate, A11 shows the advantage of a high camphor content, and the different metabolites and differentially expressed genes of the two varieties were significantly enriched in multiple metabolic pathways. Additionally, A12 contained more terpenoids and substances with aromatic odors such as sweet, fruity, floral, and green. Furthermore, a key gene (Wv_032842) regulating the acetylation of bornyl was discovered, and its significantly higher expression, in A12. In conclusion, this study has a guiding significance for the evaluation of germplasm resources and the breeding of excellent varieties of A. villosum.
Phenological Analysis of Grasses (Poaceae) in Comparison with Aerobiological Data in Moscow (Russia)
Authors: Severova Elena E. , Karaseva Vera S. , Selezneva Yulia M. , Polevova Svetlana S.
Published: August 2024

Grasses (Poaceae) produce large amounts of pollen and are among the main causes of pollinosis worldwide. Despite their morphological similarity, pollen grains of different grass species may have different allergenicities. Therefore, quantification of the roles of individual species in airborne pollen is an important task. There are very few studies on this topic, and none of them have been conducted in a temperate continental climate. Our study was carried out for three years (2020–2022) in the urban territory of Moscow (Russia) and aimed to understand what grass species contribute the most to the total pollen load of the atmosphere. The comparison of aerobiological and phenological data was based on calculating the phenological index, which is a combination of phenological parameters, pollen productivity of individual species, and their abundance. Our data showed that the decomposition of pollination curves based on the phenological index was sometimes very efficient but not always possible in temperate continental climates. The main reasons for disagreement between aerobiological and phenological data were weather conditions and lawn mowing. Not all grasses were equally important as sources of allergenic pollen. The greatest contribution to the pollen load at the beginning of the season in Moscow was made by Dactylis glomerata, and to a lesser extent by Phleum pratense and Festuca pratensis. These are the most common species, which are widespread throughout Europe. The contribution of minor components is insignificant.
Maintenance of High Phytoplankton Diversity in the Danubian Floodplain Lake over the Past Half-Century
Authors: Mihaljević Melita , Špoljarić Maronić Dubravka , Stević Filip , Žuna Pfeiffer Tanja , Zahirović Vanda
Published: August 2024

Riverine floodplains are recognized as centers of biodiversity, but due to intense anthropogenic pressures, many active floodplains have disappeared during the last century. This research focuses on the long-term changes in phytoplankton diversity in the floodplain lake situated in the Kopački Rit (Croatia), one of the largest conserved floodplains in the Middle Danube. The recent dataset from 2003 to 2016 and historical data from the 1970s and 1980s indicate high phytoplankton diversity, summarising 680 taxa for nearly half a century. The variability of species richness is driven by specific in-lake variables, particularly water temperature, water depth, total nitrogen, pH, and transparency, determined by a redundancy analysis of the current data. The high phytoplankton diversity levels are sustained regardless of intense pressures on the lake environment, including exposure to strong anthropogenic pollution in the past and extreme hydrological events, both droughts and floods, which have increasingly affected this part of the Danube in the last decades. The conserved hydrological connection between various biotopes along the river–floodplain gradient seems crucial in maintaining high phytoplankton diversity. Accordingly, conserving natural flooding is mandatory to maintain high biodiversity in complex and dynamic river–floodplain systems.
Biochemical Composition of Pumpkin Seeds and Seed By-Products
Authors: Polyzos Nikolaos , Fernandes Ângela , Calhelha Ricardo C. , Petrović Jovana , Soković Marina , Ferreira Isabel C. F. R. , Barros Lillian , Petropoulos Spyridon A.
Published: August 2024

The goal of the current work was to assess the nutritional profile and phytochemical properties of cucurbit (Cucurbita maxima L.) seeds, seed oils and oil extraction by-products (e.g., seed-cakes). Our results suggest a high nutritional value for both cucurbit seeds and cucurbit cake, while γ-tocopherol was the richest compound, with traces of α, β and δ-tocopherol compounds also detected. Regarding the free sugars composition, there were recorded significant statistical differences between seeds and cucurbit seed-cake, although sucrose content was the highest for both matrices (1.97 and 2.9 g/100 g dw, respectively) followed by trehalose (0.26 and 0.25 g/100 g dw, respectively), fructose (0.20 and 0.34 g/100 g dw, respectively) and glucose (0.21 and 0.19 g/100 g dw, respectively). In terms of organic acids, oxalic was the only compound detected in seed cake (0.006 g/100 g dw), while in seeds only traces of oxalic and malic acid were detected. In relation to fatty acid composition, linolenic acid was the most abundant compound in both seeds and seed-cake (43.9% and 41.5%, respectively), while oleic acid (37.0% and 36.3%, respectively), palmitic acid (12.2% and 14.0%, respectively) and stearic acid (4.83% and 5.46%, respectively) were detected in lesser amounts. Moreover, polyunsaturated fatty acids (PUFA) were the major fatty acids class (44.5% and 42.3% in seeds and seed cake, respectively) compared to monounsaturated fatty acids (MUFA; 37.4% and 36.7% in seeds and seed cake, respectively) and saturated fatty acids (SFA; 18.1% and 21.0% in seeds and seed cake, respectively) which were detected in lower amounts. Furthermore, the tested extracts did not present any cytotoxic or hepatoxic activity at the maximum tested concentration (GI50 > 400 μg/mL), while seed oils presented satisfactory antimicrobial properties with inhibitory activity against the studied bacterial strains and fungi. Our findings provide valuable knowledge regarding the exploitation of pumpkin seeds and seed by-products as valuable natural sources of nutrients and phytochemicals in the food industry sector within the context of a circular economy.
Injectable Hydrogel-Encapsulating Pickering Emulsion for Overcoming Lenvatinib-Resistant Hepatocellular Carcinoma via Cuproptosis Induction and Stemness Inhibition
Authors: Li Xin , Tang Chuanyu , Ye Hanjie , Fang Chihua
Published: August 2024

Lenvatinib resistance (LenR) presents a significant challenge in hepatocellular carcinoma (HCC) treatment, leading to high cancer-related mortality rates globally. Unlike traditional chemotherapy resistance mechanisms, LenR in HCC is primarily driven by increased cancer cell stemness. Disulfiram, (DSF), functioning as a Cu ionophore, can coordinate with Cu2+ to overcome LenR in HCC by inhibiting cancer cell stemness and cuproptosis. However, DSF faces challenges due to its poor water solubility, while copper ions present issues related to systemic toxicity during widespread use. To address this, DSF and CuO nanoparticles (NPs) were co-encapsulated to form an oil-in-water Pickering emulsion (DSF@CuO), effectively elevating DSF and copper ion concentrations within the tumor microenvironment (TME). DSF@CuO was then combined with sodium alginate (SA) to form a DSF@CuO-SA solution, which gelatinizes in situ with Ca2+ in the TME to form a DSF@CuO Gel, enhancing Pickering emulsion stability and sustaining DSF and copper ion release. A DSF@CuO Gel exhibits enhanced stability and therapeutic efficacy compared to conventional administration methods. It effectively induces mitochondrial dysfunction and cuproptosis in LenR HCC cells by downregulating DLAT, LIAS, and CDKN2A, while upregulating FDX1. Furthermore, it suppresses cancer stemness pathways through activation of the JNK/p38 MAPK pathway and inhibition of the NF-κB and NOTCH signaling pathways. These findings suggest that DSF@CuO Gels are a promising therapeutic strategy for treating LenR HCC. In vivo and in vitro LenR HCC models demonstrated significant therapeutic efficacy. In conclusion, this novel approach underscores DSF@CuO Gel’s potential to overcome LenR in HCC, offering a novel approach to address this clinical challenge.
Enhancing Fatigue Resistance of Polylactic Acid through Natural Reinforcement in Material Extrusion
Authors: Bermudo Gamboa Carolina , Martín-Béjar Sergio , Bañón García Fermín , Sevilla Hurtado Lorenzo
Published: August 2024

This research paper aims to enhance the fatigue resistance of polylactic acid (PLA) in Material Extrusion (ME) by incorporating natural reinforcement, focusing on rotational bending fatigue. The study investigates the fatigue behavior of PLA in ME, using various natural fibers such as cellulose, coffee, and flax as potential reinforcements. It explores the optimization of printing parameters to address challenges like warping and shrinkage, which can affect dimensional accuracy and fatigue performance, particularly under the rotational bending conditions analyzed. Cellulose emerges as the most promising natural fiber reinforcement for PLA in ME, exhibiting superior resistance to warping and shrinkage. It also demonstrates minimal geometrical deviations, enabling the production of components with tighter dimensional tolerances. Additionally, the study highlights the significant influence of natural fiber reinforcement on the dimensional deviations and rotational fatigue behavior of printed components. The fatigue resistance of PLA was significantly improved with natural fiber reinforcements. Specifically, PLA reinforced with cellulose showed an increase in fatigue life, achieving up to 13.7 MPa stress at 70,000 cycles compared to unreinforced PLA. PLA with coffee and flax fibers also demonstrated enhanced performance, with stress values reaching 13.6 MPa and 13.5 MPa, respectively, at similar cycle counts. These results suggest that natural fiber reinforcements can effectively improve the fatigue resistance and dimensional stability of PLA components produced by ME. This paper contributes to the advancement of additive manufacturing by introducing natural fiber reinforcement as a sustainable solution to enhance PLA performance under rotational bending fatigue conditions. It offers insights into the comparative effectiveness of natural fibers and synthetic counterparts, particularly emphasizing the superior performance of cellulose.
Evaluation of Shape Recovery Performance of Shape Memory Polymers with Carbon-Based Fillers
Authors: Choi Sungwoong , Jang Seongeun , Yoo Seung Hwa , Lee Gyo Woo , Choi Duyoung
Published: August 2024

This study focuses on enhancing the thermal properties and shape recovery performance of shape memory polymers (SMPs) through the application of carbon-based fillers. Single and mixed fillers were used to investigate their effects on the glass transition temperature (Tg), thermal conductivity, and shape recovery performance. The interaction among the three-dimensional (3D) structures of mixed fillers played a crucial role in enhancing the properties of the SMP. These interactions facilitated efficient heat transfer pathways and conserved strain energy. The application of mixed fillers resulted in substantial improvements, demonstrating a remarkable 290.37% increase in thermal conductivity for SMPCs containing 60 μm carbon fiber (CF) 10 wt% + graphite 20 wt% and a 60.99% reduction in shape recovery time for SMPCs containing CF 2.5 wt% + graphite 2.5 wt%. At a content of 15 wt%, a higher graphite content compared to CF improved the thermal conductivity by 37.42% and reduced the shape recovery time by 6.98%. The findings demonstrate that the application of mixed fillers, especially those with high graphite content, is effective in improving the thermal properties and shape recovery performance of SMPs. By using mixed fillers with high graphite content, the performance of the SMP showed significant improvement in situations where fast response times were required.
Ultrasound-Assisted Extrusion Compounding of Nano Clay/Polypropylene Nano Compounds
Authors: Francucci Gaston , Rodriguez Elena , Rodriguez María Eugenia
Published: August 2024

The incorporation of nanoparticles can significantly enhance the properties of polymers. However, the industrial production of nanocomposites presents a technological challenge in achieving the proper dispersion of nanoparticles within the polymer matrix. In this work, a novel device is presented that can be seamlessly integrated with standard twin-screw extruders, enabling the application of ultrasonic vibration to molten polymeric material. The primary objective of this study is to experimentally validate the effectiveness of this technology in improving the dispersion of nanoparticles. To accomplish this, a comparative analysis was carried out between nanocomposites obtained through conventional compounding extrusion and those processed with the assistance of ultrasonic vibrations. The nanocomposites under investigation consist of a polypropylene (PP) matrix reinforced with nano clays (Cloisite 20A) at a target loading ratio of 5% by weight. To comprehensively evaluate the impact of the ultrasound-assisted compounding, various key properties were assessed, such as the melt flow index (MFI) to characterize the flow behavior, mechanical properties to evaluate the structural performance, oxygen barrier properties to assess potential gas permeability, and microstructure analysis using Scanning Electron Microscopy (SEM) for detailed morphology characterization. The results suggested an improvement in nanoparticle dispersion when using the ultrasound device, particularly when the intensity was adjusted to 60%.
Novel Recycling, Defibrillation, and Delignification Methods for Isolating α-Cellulose from Different Lignocellulosic Precursors for the Eco-Friendly Fiber Industry
Authors: Hindi Sherif S.
Published: August 2024

Alpha-cellulose, a unique, natural, and essential polymer for the fiber industry, was isolated in an ecofriendly manner using eleven novel systems comprising recycling, defibrillation, and delignification of prosenchyma cells (vessels and fibers) of ten lignocellulosic resources. Seven hardwood species were selected, namely Conocorpus erectus, Leucaena leucocephala, Simmondsia chinensis, Azadirachta indica, Moringa perigrina, Calotropis procera, and Ceiba pentandra. Moreover, three recycled cellulosic wastes were chosen due to their high levels of accumulation annually in the fibrous wastes of Saudi Arabia, namely recycled writing papers (RWPs), recycled newspapers (RNPs), and recycled cardboard (RC). Each of the parent samples and the resultant alpha-cellulose was characterized physically, chemically, and anatomically. The properties examined differed significantly among the ten resources studied, and their mean values lies within the cited ranges. Among the seven tree species, L. leucocephala was the best cellulosic precursor due to its higher fiber yield (55.46%) and holocellulose content (70.82%) with the lowest content of Klasson lignin (18.86%). Moreover, RWP was the best α-cellulose precursor, exhibiting the highest holocellulose (87%) and the lowest lignin (2%) content. Despite the high content of ash and other additives accompanied with the three lignocellulosic wastes that were added upon fabrication to enhance their quality (10%, 11%, and 14.52% for RWP, RNP, and RC, respectively), they can be considered as an inexhaustible treasure source for cellulose production due to the ease and efficiency of discarding their ash minerals using the novel CaCO3-elimination process along with the other innovative techniques. Besides its main role for adjusting the pH of the delignification process, citric acid serves as an effective and environmentally friendly additive enhancing lignin breakdown while preserving cellulose integrity. Comparing the thermal behavior of the ten cellulosic resources, C. procera and C. pentandra exhibited the highest moisture content and void volume as well as having the lowest specific gravity, crystallinity index, and holocellulose content and were found to yield the highest mass loss during their thermal degradation based on thermogravimetric and differential thermal analysis in an inert atmosphere. However, the other resources used were found to yield lower mass losses. The obtained results indicate that using the innovative procedures of recycling, defibrillation, and delignification did not alter or distort either the yield or structure of the isolated α-cellulose. This is a clear indicator of their high efficiency for isolating cellulose from lignocellulosic precursors.
Environmental Assessment of Solid Recovered Fuel Production from Screening Waste Using a Life Cycle Assessment Approach
Authors: De la Torre Bayo Juan Jesús , Zamorano Montserrat , Torres-Rojo Juan C. , Pennellini Sara , Martín-Pascual Jaime , Bonoli Alessandra
Published: August 2024

The circular economy, as a new model of waste management through energy self-sufficiency and valorisation, can be applied to wastewater treatment plants (WWTPs). Screening waste from WWTP pretreatment is the only waste that is not energetically recovered and thus constrains the achievement of zero waste. Previous studies demonstrated the technical feasibility of producing solid recovered fuel (SRF) from this waste. Environmental benefits, including waste reduction, resource conservation, or reduced greenhouse gas emissions are analysed in this work. Environmental impact is quantified using the life cycle assessment (LCA) methodology through the SimaPro 9.2. software and the CML-IA baseline v3.08 impact methodology, that propose 11 impact categories. Five scenarios were established to compare current landfill disposal with the production of densified and non-densified SRF using solar and thermal drying. Within the system boundaries studied, from waste generation to SRF production, results show that landfill is the most environmentally damaging option while producing non-densified SRF using solar drying is the most environmentally viable scenario.
The Green Synthesis of Nanostructured Silicon Carbides (SiCs) from Sugarcane Bagasse Ash (SCBA) as Anodes in Lithium-Ion (Li-Ion) Batteries: A Review Paper
Authors: Pesulo Sandy U. , September Lyle A. , Kheswa Ntombizonke , Seroka Ntalane S. , Khotseng Lindiwe
Published: August 2024

Silicon is a promising anode material for the increased performance of lithium-ion batteries because of its high elemental composition and specific capacity. The application of silicon on a commercial scale is restricted due to the limitation of volume expansion. Silicon is also expensive, making it difficult for large-scale commercialisation. Different methods were used to address these issues, including a sintering process and the sol–gel method, to form silicon carbide (SiC), a hard chemical compound containing silicon and carbon. The silicon carbide anode not only acts as a buffer for volume expansion but also allows for better infiltration of the electrolyte, increasing charge and discharge capacity in the battery. Like silicon, silicon carbides can be costly. The development of renewable energy systems is very important, especially in the development of energy storage systems that are not only efficient but also cost-friendly. The cost of the energy storage devices is lowered, making them easily accessible. Silicon carbides can be synthesised from sugarcane, which is the fibrous waste that remains after juice extraction. This could be beneficial, as we could never run out of such a resource, and it offers low carbon with a high surface area. Silicon carbides can be synthesised by carbothermal reduction of silica from sugarcane bagasse. This review provides a comprehensive understanding of silicon carbides and synthetic processes. The innovative use of waste to synthesise materials would reduce costs and comply with Sustainable Development Goals (SDGs) 7 (affordable and clean energy) and 13 (climate action).
Stability Analysis of Borehole Walls When Drilling with Normal-Temperature Drilling Fluids in Permafrost Strata
Authors: Shi Jihui , Li Yang , Yan Chuanliang , Xue Mingyu
Published: August 2024

Permafrost is a temperature-sensitive geological formation characterized by low elasticity and high plasticity. Inappropriate engineering design during borehole drilling in permafrost can result in the collapse of surrounding strata. To evaluate the stability of borehole walls, a finite element model was developed based on the inherent physical properties of permafrost. This model was utilized to investigate the thermal, stress, and plastic yield zone evolution around the borehole during drilling with normal-temperature fluids. The borehole expansion rate was employed as a quantitative measure to assess wall stability. The analysis reveals that the strata adjacent to the borehole, when drilled with normal-temperature fluids, experience thawing and yielding, with secondary stress concentrations in unthawed strata driving the progressive expansion of the plastic zone. The degree of plastic deformation diminishes with increasing distance from the borehole. Consequently, the borehole expansion rate was utilized to evaluate collapse risk under varying conditions, including permafrost thickness, depth, plastic strain thresholds, and drilling fluid densities. The findings suggest that normal-temperature drilling fluids are appropriate for thin permafrost layers, whereas for thicker permafrost, adjustments in drilling fluid density are required to ensure the stability of borehole walls due to the elevated temperatures and geostress at greater depths.
Numerical Investigation of Inlet Height and Width Variations on Separation Performance and Pressure Drop of Multi-Inlet Cyclone Separators
Authors: Barua Saikat , Batcha Mohd Faizal Mohideen , Mohammed Akmal Nizam , Saif Yazid , Al-Alimi Sami , Al-fakih Mohammed A. M. , Zhou Wenbin
Published: August 2024

Multi-inlet cyclone separators can play a vital role in industrial processes by enhancing particle separation efficiency and minimizing energy consumption. This numerical study investigates multi-inlet cyclone separators to enhance their performance using a constant flow rate with a varying inlet height and width. By systematically varying the inlet height and width, three-inlet and four-inlet cyclone separators are developed and evaluated, termed 3 inlet-a, 4 inlet-a, 3 inlet-b, and 4 inlet-b. The findings reveal that increasing the number of inlets without changing the total inlet area does not improve the separation performance. However, strategic modifications to the inlet height and width significantly enhance the separation efficiency. Notably, the 3 inlet-a and 4 inlet-a designs achieve higher separation efficiencies at a 1.22 m3/s flow rate without increasing the pressure drop. Meanwhile, the 3 inlet-b and 4 inlet-b models demonstrate superior performances, with a higher separation efficiency and a pressure drop only marginally higher than the two-inlet design. This study provides valuable insights into the impact of inlet variations on cyclone separator performance, guiding future efforts to enhance the separation efficiency in multi-inlet designs.
Integrating AI and Blockchain for Enhanced Data Security in IoT-Driven Smart Cities
Authors: Khan Burhan Ul Islam , Goh Khang Wen , Khan Abdul Raouf , Zuhairi Megat F. , Chaimanee Mesith
Published: August 2024

Blockchain is recognized for its robust security features, and its integration with Internet of Things (IoT) systems presents scalability and operational challenges. Deploying Artificial Intelligence (AI) within blockchain environments raises concerns about balancing rigorous security requirements with computational efficiency. The prime motivation resides in integrating AI with blockchain to strengthen IoT security and withstand multiple variants of lethal threats. With the increasing number of IoT devices, there has also been a spontaneous increase in security vulnerabilities. While conventional security methods are inadequate for the diversification of IoT devices, adopting AI can assist in identifying and mitigating such threats in real time, whereas integrating AI with blockchain can offer more intelligent decentralized security measures. The paper contributes to a three-layered architecture encompassing the device/sensory, edge, and cloud layers. This structure supports a novel method for assessing legitimacy scores and serves as an initial security measure. The proposed scheme also enhances the architecture by introducing an Ethereum-based data repositioning framework as a potential trapdoor function, ensuring maximal secrecy. To complement this, a simplified consensus module generates a conclusive evidence matrix, bolstering accountability. The model also incorporates an innovative AI-based security optimization utilizing an unconventional neural network model that operates faster and is enhanced with metaheuristic algorithms. Comparative benchmarks demonstrate that our approach results in a 48.5% improvement in threat detection accuracy and a 23.5% reduction in processing time relative to existing systems, marking significant advancements in IoT security for smart cities.