bluuebunny/crossref_metadata_2025 huggingface dataset:
Emissions and Fire Risk Assessment of Nitrocellulose as a Sustainable Alternative in Pyrotechnic Compositions
Authors: León David , Amez Isabel , Radojević Miloš , Manić Nebojša , Stojiljković Dragoslava , Milivojević Aleksandar , García-Torrent Javier , Castells Blanca
Published: August 2024

The use of fireworks as a sign of celebration is a common practice in many countries. During the last few years, this sector has been subjected to various social criticisms that have even led to a ban on their use in different places. One of the main causes is the environmental pollution they generate, due to emissions from combustion reactions associated with the use of pyrotechnic devices. Nitrate ester-based compounds as propellants are used in other solid rockets such as ammunition, but their use in pyrotechnics is currently limited to a small number of fireworks. This research analyses the substitution of black powder with nitrocellulose ([C6H7(NO2)3O5]n) as a propellant for aerial articles, as well as to the use of nitrocellulose as a perchlorate-free compound. In addition to achieving better clarity effects due to less smoke, the use of nitrocellulose reduces greenhouse gas emissions. However, this nitrogen-rich compound is more unstable, and the ignition sensitivity is higher. Therefore, the benefits and risks associated with this alternative compound need to be evaluated, but the evidence shown suggests a promising direction for the sustainable evolution of pyrotechnic compositions.
A Comparison of Polyvalent Passive Immunoprotection from Antibodies with Different Immunity Models of Live or Inactivated Vibrio fluvialis in Fish
Authors: Xiao Huihui , Cui Pan , Chen Jing , Han Xiaohui , Ma Ziye , Chen Chen , Liu Yong , Liu Xiang
Published: August 2024

Polyvalent passive vaccines can act fast in resisting various bacteria with good efficacy, and they have application value in aquaculture. This study prepared live and inactivated Vibrio fluvialis mouse antisera (L-VF-antiserum and I-VF-antiserum), and administered them to goldfish (Carassius auratus) infected with V. fluvialis and Aeromonas hydrophila. The passive protective rates of live and inactivated mouse antisera against V. fluvialis were 60% (p < 0.05) and 40% (p < 0.05), and their passive cross-protective rates against A. hydrophila were 42.86% (p < 0.05) and 35.71% (p < 0.05), respectively. Furthermore, the two mouse antisera could recognize the bacteria in vitro; the content of bacteria in the C. auratus kidney decreased (p < 0.05), the phagocytic activity of C. auratus leukocytes was enhanced (p < 0.05), and the expression of inflammatory genes and activity of antioxidant factors decreased (p < 0.05). Moreover, the kidney, spleen, and intestinal tissue structures were intact, and the apoptosis and DNA damage were decreased (p < 0.05) among the kidney cells. The live V. fluvialis antiserum contained more antibodies against the outer membrane proteins of V. fluvialis than the inactivated mouse antiserum. The immunoprotective abilities of the live V. fluvialis antiserum were higher than those of the inactivated antiserum, and the antiserum of live V. fluvialis immunity demonstrated potential application value as a polyvalent passive immune vaccine in fish.
The Stressogenic Impact of Bacterial Secretomes Is Modulated by the Size of the Milk Fat Globule Used as a Substrate
Authors: Tzirkel-Hancock Noam , Raz Chen , Sharabi Lior , Argov-Argaman Nurit
Published: August 2024

Milk fat globules (MFGs) are produced by mammary epithelial cells (MECs) and originate from intracellular lipid droplets with a wide size distribution. In the mammary gland and milk, bacteria can thrive on MFGs. Herein, we aimed to investigate whether the response of MECs to the bacterial secretome is dependent on the MFG size used as a substrate for the bacteria, and whether the response differs between pathogenic and commensal bacteria. We used secretomes from both Bacillus subtilis and E. coli. Proinflammatory gene expression in MECs was elevated by the bacteria secretomes from both bacteria sources, while higher expression was found in cells exposed to the secretome of bacteria grown on large MFGs. The secretome of B. subtilis reduced lipid droplet size in MECs. When the secretome originated from E. coli, lipid droplet size in MEC cytoplasm was elevated with a stronger response to the secretome from bacteria grown on large compared with small MFGs. These results indicate that MEC response to bacterial output is modulated by bacteria type and the size of MFGs used by the bacteria, which can modulate the stress response of the milk-producing cells, their lipid output, and consequently milk quality.
Consumption Patterns and Willingness to Pay for Sustainable Aquatic Food in China
Authors: Xu Hao , Wu Tianqi , Budhathoki Mausam , Fang Dingxi Safari , Zhang Wenbo , Wang Xin
Published: August 2024

China, as the world’s largest producer, trader, and consumer of aquatic foods, lacks comprehensive research on consumption patterns and willingness to pay for sustainable aquatic food. This study addressed this gap through an online survey of 3403 participants across Chinese provinces. A majority of consumers (34.7% of the participants) consume aquatic food twice or more per week, mainly from traditional markets (26%). Most prefer fresh or live products (76%), with 42% seeing no difference between farmed and wild options. Consumption is higher among older, affluent, urban, and coastal residents. Crustaceans, especially shrimp, are frequently consumed species, with growing interest in luxury species like salmon and abalone. Taste and quality emerge as the primary factors motivating consumer choices in aquatic food purchases. Food safety is the primary concern, followed by environmental impact. Notably, 92.4% of participants would pay extra for certified products. Factors influencing a higher willingness to pay include higher income, inland residence, price sensitivity, origin consciousness, and concerns about food safety and the environment. The findings highlight that China’s aquatic food industry and consumption can become more sustainable by aligning with consumer preferences for high-quality and diverse aquatic food through both production and import, while also addressing concerns related to food safety and environmental impact. This research provides valuable insights into China’s rapidly transforming aquatic food market landscape, offering implications for industry innovation and the promotion of sustainable consumption patterns.
Forensic Entomological Examinations for Animal Welfare Offices under Suboptimal Preservation Conditions
Authors: Baumjohann Kristina , Benecke Mark
Published: August 2024

A female dog had allegedly been alive one day before its death. The veterinary office thought about accusing the dog’s owner for animal cruelty and commissioned forensic entomological expertise for the calculation of the time of insect colonization on the dog’s body. The statement of the dog’s owner was proven false by us on the grounds of (a) the advanced state of decomposition and (b) the minimal developmental time of fly larvae found on the dog’s body. The darkening and deformation of the fly maggots as well as insufficient temperature data made case work trickier than usual. We worked through the case by creating forensic entomological temperature scenarios. The court used the entomological evidence and issued a penalty order.
The Market for Low-Carbon-Intensity Ammonia
Authors: Wang Haoying , Lin Ning , Arzumanyan Mariam
Published: August 2024

As carbon capture and storage (CCS) technologies mature, the concept of a low-carbon or net-zero-carbon economy becomes more and more feasible. While many chemical and energy products do not contain carbon in their compounds, the upstream production process does. An added CCS module allows the removal of carbon emissions from the production process, which expands the value chain. This paper focuses on one of such commodities—low-carbon-intensity ammonia (LCIA). Even though ammonia is carbon-free in its final product, it is commonly made from natural gas, and the production process could generate significant carbon emissions. The idea of LCIA is to reduce the carbon footprint of the ammonia production process (e.g., blue ammonia) or eliminate carbon from the production process (e.g., green ammonia via electrolysis) so that the entire supply chain is decarbonized. The goal of this paper is two-fold. We first explore the US domestic market and the international market for LCIA. We then discuss relevant federal and local policies that could help grow markets for LCIA. The agricultural sector will be the center of the discussion, which consumes an estimated 70–90% of the global ammonia supply as fertilizers. The paper also examines other potential uses of LCIA, such as alternative fuels for decarbonizing agricultural machinery and transportation sectors. Finally, we argue that developing a comprehensive LCIA value chain, supported by dedicated policy measures and broad stakeholder engagement, is critical for materializing the potential of LCIA in contributing to a climate-resilient and sustainable economy.
Are There Barriers Separating the Pink River Dolphin Populations (Inia boliviensis, Iniidae, Cetacea) within the Mamoré–Iténez River Basins (Bolivia)? An Analysis of Its Genetic Structure by Means of Mitochondrial and Nuclear DNA Markers
Authors: Ruiz-García Manuel , Escobar-Armel Pablo , Martínez-Agüero María , Gaviria Magda , Álvarez Diana , Pinedo Myreya , Shostell Joseph Mark
Published: August 2024

The pink river dolphin, or bufeo, is one of the dolphins which lives in the rivers of the Orinoco and Amazon basins in South America. The Bolivian bufeo population is considered a differentiated species (Inia boliviensis) from the Amazon and Orinoco species (Inia geoffrensis). Until now, no study has completed an extensive population genetics analysis of the bufeo in Bolivian rivers. We analyzed 82 bufeos from different rivers from the Mamoré and Iténez (Guaporé) river basins for the mt control region (CR), nuclear microsatellites, and DQB-1 gene sequences to determine if the inner rapids of these Bolivian river basins have some influence on the genetic structure of this species. The first relevant result was that the genetic diversity for CR, and the microsatellites were substantially lower in the Bolivian bufeos than in the dolphins studied in other areas of the Amazon and Orinoco. However, the DQB-1 gene sequences yielded similar genetic diversity to those found in other areas. The second relevant result is the existence of some significant genetic heterogeneity among the bufeo populations within Bolivia, although in a small degree, but this differentiation is independent of the inner rapids of the Bolivian rivers we sampled. The third relevant result was the existence of significant isolation by distance for the CR, but not for microsatellites and DQB-1 gene sequences. This was related to differential gene flow capacity of females (philopatric) and males (less philopatric and more migrants) and, possibly, to different selective patterns affecting the molecular markers studied. The fourth relevant result was related to diverse demographic changes of these bufeos. At least two or three bottleneck events and one or two population expansions have occurred in the Bolivian bufeo population. The major part of these events occurred during the Pleistocene.
Lung Cancer: New Directions in Senior Patients Assessment
Authors: Pîslaru Anca Iuliana , Albișteanu Sabinne-Marie , Ilie Adina Carmen , Ștefaniu Ramona , Mârza Aurelia , Moscaliuc Ștefan , Nicoară Mălina , Turcu Ana-Maria , Grigoraș Gabriela , Alexa Ioana Dana
Published: August 2024

Age is but one significant prognostic factor in lung cancer, influencing survival, treatment response, and outcomes. This narrative review synthesizes findings from searches of 11 leading databases of research studies, systematic reviews, book chapters, and clinical trial reports on lung cancer in senior patients, with a focus on geriatric assessment as well as biomarkers. Key prognostic factors for lung cancer in seniors include biological age, functional capability, physical and psychological comorbidities, frailty, nutrition, status, and biomarkers like DNA methylation age. We identified the most valuable assessments that balance efficacy with quality of life. Optimizing care and improving outcomes with senior lung cancer patients benefits from a tailored therapeutic approach incorporating a complex geriatric assessment. A multidisciplinary collaboration between geriatricians, oncologists, and pulmonologists is crucial.
Gout Basic Research: 2023 in Review
Authors: Muntiu Maria , Joosten Leo A. B. , Crişan Tania O.
Published: August 2024

Gout is a prevalent form of inflammatory arthritis caused by the crystallization of uric acid in the joints and soft tissues, leading to acute, painful attacks. Activation of the NLRP3 inflammasome in mononuclear cells, along with inflammasome-independent pathways, is responsible for the inflammatory phenotype in gout. Research into the different aspects of gout pathophysiology and potential treatment options is ongoing. This review highlights some of the basic research published in the 12 months following the 2022 Gout, Hyperuricemia, and Crystal-Associated Disease Network (G-CAN) conference and focuses on mechanisms of inflammation, encompassing pro- and anti-inflammatory pathways, as well as the exploration of various biological systems, such as single-cell transcriptomics, proteomics, metabolomics, and microbiome analyses.
Does Endodontic Treatment Influence Human Endocarditis? Systematic Review
Authors: Pereira Jennifer Santos , Leutz Anna Carolina Neves , Gomes Brenda P. F. A. , Pelepenko Lauter E. , Marciano Marina Angélica
Published: August 2024

Endocarditis is a severe infection affecting the heart’s inner layer, the endocardium. Its pathophysiology may involve heart valve damage, bacteria adhesion and biofilm formation, potentially leading to fatal complications. Bacteria from various sources, including from endodontic diseases and its treatments may enter the bloodstream provoking this condition. This systematic review aimed to explore the influence of endodontic factors on endocarditis. Searches across PubMed, Embase, Cochrane Library and manual sources yielded 14 relevant articles from 1562 screened studies. Assessment platforms from JBI Critical Appraisal Tools evaluated studies biases. Findings mainly focused on transient bacteraemia as a key indicator of risk correlating bacterial virulence and counts with endocarditis development. Worryingly, multi-species bacteraemia post-endodontic treatment was noted including the genera Enterococcus, Parvimonas, Streptococcus and Staphylococcus. Conclusive validation of the incidence and association between endodontic patients and endocarditis was limited due to a lack of robust longitudinal investigations, such as randomized controlled trials. This emphasizes the need for further research with well-designed methodologies to provide a full understanding of the causative bacterial population and its pathological mechanisms. A current guideline (2023 European Society of Cardiology) was developed to support healthcare professionals in diagnosing and managing infective endocarditis; this 2023 version is introducing a new diagnostic algorithm to aid in patient classification aiming to improve outcomes for this challenging disease. The study was a priori registered on PROSPERO (CRD42023407736).
Assessment of the Impact of Annual Growing Conditions on the Physicochemical Properties of Mango Kernel Fat
Authors: Kouassi Alfred Kouakou , Alabi Taofic , Purcaro Giorgia , Blecker Christophe , Danthine Sabine
Published: August 2024

In this study, the effect of growing conditions in different harvest years on the physicochemical properties of various Ivorian mango kernel fat (MKF) varieties was investigated. The fats extracted from mango kernels were analysed with respect to their fatty acid composition (FAC) and triacylglycerol (TAG) composition, melting profile, and solid fat content (SFC). The results indicate that variations in MKF content between non-consecutive harvest years (2021 and 2023) were influenced by environmental conditions, particularly rainfall and genetic factors, demonstrating the diverse response to environmental changes. Traditional varieties showed a decrease in fat content in the drier year (2023), while commercial varieties exhibited an increase. FAC was also affected, with changes in oleic- and stearic-acid levels, depending on water availability. This impacted the TAG composition, which in turn influenced the physical characteristics of the MKF. These findings highlight the importance of climatic factors in determining the quality and characteristics of MKF, which have significant implications for industrial applications. This suggests that it is necessary to take into account such factors when implementing logistic chains for the supply of quality raw materials.
Life-History Traits of the Bluespotted Cornetfish Fistularia commersonii Rüppell, 1838 in Rhodes, Greece, with Notes on the Red Cornetfish Fistularia petimba Lacepède, 1803
Authors: Kondylatos Gerasimos , Theocharis Alexandros , Charokopou Magdalini , Perakis Emmanouil , Mavrouleas Dimitrios , Kalaentzis Konstantinos , Pafras Dimitris , Klaoudatos Dimitris
Published: August 2024

In the Mediterranean, the bluespotted cornetfish Fistularia commersonii Rüppell, 1838, presents a minor socioeconomic impact and the assessment of any environmental impact requires more relevant data. The congeneric red cornetfish Fistularia petimba Lacepède, 1803, has expanded its distribution range within the basin but only small numbers have been reported to date. A total of 207 individuals of F. commersonii were collected between April 2021 and March 2022 from the Levantine coast of Rhodes and 92 more from various locations. Additionally, 13 individuals of Fistularia petimba were caught in March 2024 from the Aegean coasts of the island. We aim to assess the current progression of the population of the two cornetfish, the possible further exploitation of F. commersonii to boost the local fishing economy, their possible dietary overlaps and to add valuable biological and ecological data. In F. commersonii, male to female ratio (1:1.33) significantly departed from 1:1, with length–weight relationships exhibiting positive allometric growth. Six age groups were identified. The highest reproductive intensity was observed during summer. The onset of sexual maturity was estimated at 65.52 cm in total length (1.8 years). Longevity was estimated at 11.1 years with females growing larger than males. The exploitation rate (E = 0.47) indicated that the population is underexploited. The optimum and target fishing mortality were higher in comparison with the present fishing mortality (F = 0.48), indicating a potential for commercial exploitation of the species. For F. petimba, the sex ratio was 1:2.25. The species preys on crustaceans, followed by fish and molluscs. The finding of Vanderhorstia mertensi (Klausewitz, 1974) in the stomach content of both cornetfishes constitutes the second published record for Hellenic waters and the first for the Dodecanese Islands.
Leukemia Incidence by Occupation and Industry: A Cohort Study of 2.3 Million Workers from Ontario, Canada
Authors: Samsel Konrad , Navaneelan Tanya , DeBono Nathan , Everest Louis , Demers Paul A. , Sritharan Jeavana
Published: August 2024

Although a significant body of evidence has attributed certain occupational exposures with leukemia, such as benzene, formaldehyde, 1,3-butadiene and ionizing radiation, more research is needed to identify work environments at increased risk for this disease. Our study aimed to identify occupational and industry groups associated with an elevated incidence of leukemia using a diverse cohort of workers’ compensation claimants from Ontario, Canada. A total of 2,363,818 workers in the Occupational Disease Surveillance System (ODSS) cohort, with claims between 1983–2019, were followed for malignant leukemia diagnoses up to 31 December 2019. We used a Cox proportional-hazards model to estimate the relative incidence of leukemia in specific occupation and industry groups. After adjusting for age and birth year, males in protective services (HR = 1.17, 95% CI = 1.02–1.35), metal machining (HR = 1.23, 95% CI = 1.07–1.41), transport (HR = 1.15, 95% CI = 1.06–1.25), and mining occupations (HR = 1.28, 95% CI = 1.02–1.60) had elevated risks of leukemia compared to other workers in the ODSS, with comparable findings by industry. Among female workers, slight risk elevations were observed among product fabricating, assembling, and repairing occupations, with other increased risks seen in furniture and fixture manufacturing, storage, and retail industries. These findings underscore the need for exposure-based studies to better understand occupational hazards in these settings.
Associations between Cannabis Consumption Methods and Cannabis Risk Perception
Authors: Choi Namkee G. , Marti C. Nathan , Choi Bryan Y.
Published: August 2024

Given diversified cannabis products, we examined associations between cannabis consumption methods and cannabis risk perception of smoking cannabis 1–2 times a week. Using the 2022 U.S. National Survey on Drug Use and Health data (N = 12,796 past-year adult cannabis users; M = 6127 and F = 6669), we used multinomial and binary logistic regression models. Smoking was the most prevalent method, followed by eating/drinking, vaping, and dabbing. One-half of cannabis users reported no perceived risk of smoking cannabis 1–2 times a week, 37.5% perceived slight risk, 9.2% moderate risk, and 2.9% great risk. Those with moderate or great risk perception had a lower likelihood of using 4+ methods of consumption (e.g., RRR = 0.40, 95% CI = 0.20, 0.77 for great risk perception). Any perceived risk was associated with higher odds of edibles/drinks only (e.g., aOR = 2.81, 95% CI = 1.43, 5.54 for great risk perception). Along with medical use and CUD, sociodemographic factors, mental illness, and other substance use were also significant correlates of cannabis consumption methods. Understanding the varying risk perceptions associated with different consumption methods is needed for harm reduction initiatives. More research is needed on cannabis products, particularly edibles/drinks and dabs/concentrates, to better understand the potential risks associated with them.
Manager–Team (Dis)agreement on Stress-Preventive Behaviours: Relationship with Psychosocial Work Environment and Employees’ Well-Being
Authors: Toderi Stefano , Cioffi Glauco , Yarker Joanna , Lewis Rachel , Houdmont Jonathan , Balducci Cristian
Published: August 2024

The “Management Competencies to Prevent and Reduce Stress at Work” (MCPARS) approach focuses on identifying the stress-preventive managers’ competencies able to optimise the employees’ well-being through the management of the psychosocial work environment. Considering leadership as contextualised in complex social dynamics, the self–other agreement (SOA) investigation of the MCPARS may enhance previous findings, as it allows for exploring the manager–team perceptions’ (dis)agreement and its potential implications. However, no studies have tested the MCPARS using the SOA and multisource data. Grounded in Yammarino and Atwater’s SOA reference theory, we conducted an in-depth investigation on the MCPARS’s theoretical framework by examining the implications of manager–team (dis)agreement, regarding managers’ competencies, on employees’ psychosocial environment (H1–H2) and affective well-being (H3). Data from 36 managers and 475 employees were analysed by performing several polynomial regressions, response surface, and mediation analyses. The results reveal a significant relationship between SOA on MCPARS and employees’ perceptions of the psychosocial environment (H1). Employees report better perceptions when supervised by in-agreement good or under-estimator managers, while lower ratings occur under over-estimator or in-agreement poor managers (H2). Moreover, the psychosocial environment significantly mediated the relationship between SOA on MCPARS and employees’ well-being (H3). The MCPARS theoretical model’s soundness is supported, and its implications are discussed.
Empowering and Building the Capabilities of Mid-Level Health Service Managers to Lead and Support the Health Workforce—A Study Protocol
Authors: Liang Zhanming , King Jemma C. , Nagle Cate , Pain Tilley , Mallett Andrew J.
Published: August 2024

(1) Background: Mid-level managers in healthcare are central to improving safety and quality of care. Their ability in demonstrating leadership and management competency in their roles and supporting frontline managers and frontline staff has a direct effect on staff retention and turn-over. Yet, investment in their professional development and support for mid-level managers is often neither adequate nor effective, and high rates of staff turnover are evident. This study, set in northern Queensland, Australia, takes a strength-based approach to explore the role and strengths of mid-level managers and organisations’ existing mechanisms in supporting managers. With broad involvement and contribution from managers at different management level and frontline staff, the project will identify strategies to address the challenges mid-level managers face while building on their capabilities. (2) Methods: Using co-design principles, a situation analysis approach will guide a mixed-methods, multiphase design. Qualitative data will be collected using transcripts of focus groups and quantitative data will be collected by surveys that include validated scales. (3) Results: Thematic analysis of the transcripts will be guided by the framework of Braun and Clarke. Quantitative data will employ descriptive and inferential analysis, including chi-squared, t-tests, and univariate analyses of variance. (4) Conclusions: This study will generate evidence to guide two partner organisations, and other similar organisations, to develop strategies to improve support for mid-level managers and build their capabilities to support and lead frontline managers and staff. Competent mid-level managers are critical to high-quality patient care and improve the outcomes of the population they serve.
Start of the Season in a Seasonal Work Context: A Better Understanding of the Difficulties Experienced by Seasonal Workers in the Food Processing Industry for the Prevention of Work-Related Musculoskeletal Disorders
Authors: Goupil Audrey , Major Marie-Eve
Published: August 2024

The specific period of the start of a new working season and a return to work after the off-season seems to be a critical moment for the musculoskeletal health of seasonal workers. This study aims to identify the difficulties and working conditions encountered by seasonal workers in this particular period of the working season which may increase the risk of work-related musculoskeletal disorders (WMSDs). An in-depth ergonomic work activity study, combined with a multiple case study of eight seasonal workers from a meat processing facility, was conducted. Various interviews (n = 24) and observations of work activity, organization, and production (n = 96 h) were held at different moments (off-season, return to work at the start of the season, and during the season). Critical work situations exposing workers to WMSD risks emerged and highlighted a diversity of difficulties, such as accomplishing work activity involving strong physical strain and a significant and underestimated mental load, and having to rapidly develop new skills or re-learn working strategies after a long off-period. The study findings have implications for developing actions to prevent WMSDs that target working conditions and support a return to work for returning seasonal workers and a start of work for new seasonal workers, and to address work disability in this context.
Factors Influencing the Efficiency of Demand-Responsive Transport Services in Rural Areas: A GIS-Based Method for Optimising and Evaluating Potential Services
Authors: Tejero-Beteta Carlos , Moyano Amparo , Sánchez-Cambronero Santos
Published: August 2024

Demand-responsive transport (DRT) could be an alternative for extending the accessibility of high-speed rail (HSR) servicing cities in rural environments, where fixed public transport does not provide efficient services. This paper proposes a method to analyse the factors that influence the implementation of DRT systems for inter-urban mobility, connecting and integrating towns in rural areas. Methodologically, a vehicle routing problem analysis in a GIS-based environment is applied to a theoretical case study to evaluate the factors that influence DRT efficiency in different scenarios, considering the specific singularities of this kind of inter-urban long-distance mobility. The results suggest the optimal DRT solutions in these rural contexts to be those that, after adjusting the fleet to specific demands, use low-capacity vehicles, which are much better adapted to the geography of sparsely populated areas. Moreover, in adapting DRT systems to HSR travellers’ needs, windows catering to these needs should incorporate the option of setting the pickup or arrival times. This paper demonstrates that DRT systems could reach significant levels of service in rural areas compared with fixed lines and even private vehicles, especially when evaluating key aspects of the system’s efficiency for its implementation.
Increased Oxidative Stress and Decreased Citrulline in Blood Associated with Severe Novel Coronavirus Pneumonia in Adult Patients
Authors: Tsuge Mitsuru , Ichihara Eiki , Hasegawa Kou , Kudo Kenichiro , Tanimoto Yasushi , Nouso Kazuhiro , Oda Naohiro , Mitsumune Sho , Kimura Goro , Yamada Haruto , Takata Ichiro , Mitsuhashi Toshiharu , Taniguchi Akihiko , Tsukahara Kohei , Aokage Toshiyuki , Hagiya Hideharu , Toyooka Shinichi , Tsukahara Hirokazu , Maeda Yoshinobu
Published: August 2024

This study investigated the correlation between oxidative stress and blood amino acids associated with nitric oxide metabolism in adult patients with coronavirus disease (COVID-19) pneumonia. Clinical data and serum samples were prospectively collected from 100 adult patients hospitalized for COVID-19 between July 2020 and August 2021. Patients with COVID-19 were categorized into three groups for analysis based on lung infiltrates, oxygen inhalation upon admission, and the initiation of oxygen therapy after admission. Blood data, oxidative stress-related biomarkers, and serum amino acid levels upon admission were compared in these groups. Patients with lung infiltrations requiring oxygen therapy upon admission or starting oxygen post-admission exhibited higher serum levels of hydroperoxides and lower levels of citrulline compared to the control group. No remarkable differences were observed in nitrite/nitrate, asymmetric dimethylarginine, and arginine levels. Serum citrulline levels correlated significantly with serum lactate dehydrogenase and C-reactive protein levels. A significant negative correlation was found between serum levels of citrulline and hydroperoxides. Levels of hydroperoxides decreased, and citrulline levels increased during the recovery period compared to admission. Patients with COVID-19 with extensive pneumonia or poor oxygenation showed increased oxidative stress and reduced citrulline levels in the blood compared to those with fewer pulmonary complications. These findings suggest that combined oxidative stress and abnormal citrulline metabolism may play a role in the pathogenesis of COVID-19 pneumonia.
CDC20 Holds Novel Regulation Mechanism in RPA1 during Different Stages of DNA Damage to Induce Radio-Chemoresistance
Authors: Gao Yang , Wen Pengbo , Shao Chenran , Ye Cheng , Chen Yuji , You Junyu , Su Zhongjing
Published: August 2024

Targeting CDC20 can enhance the radiosensitivity of tumor cells, but the function and mechanism of CDC20 on DNA damage repair response remains vague. To examine that issue, tumor cell lines, including KYSE200, KYSE450, and HCT116, were utilized to detect the expression, function, and underlying mechanism of CDC20 in radio-chemoresistance. Western blot and immunofluorescence staining were employed to confirm CDC20 expression and location, and radiation could upregulate the expression of CDC20 in the cell nucleus. The homologous recombination (HR) and non-homologous end joining (NHEJ) reporter gene systems were utilized to explore the impact of CDC20 on DNA damage repair, indicating that CDC20 could promote HR repair and radio/chemo-resistance. In the early stages of DNA damage, CDC20 stabilizes the RPA1 protein through protein-protein interactions, activating the ATR-mediated signaling cascade, thereby aiding in genomic repair. In the later stages, CDC20 assists in the subsequent steps of damage repair by the ubiquitin-mediated degradation of RPA1. CCK-8 and colony formation assay were used to detect the function of CDC20 in cell vitality and proliferation, and targeting CDC20 can exacerbate the increase in DNA damage levels caused by cisplatin or etoposide. A tumor xenograft model was conducted in BALB/c-nu/nu mice to confirm the function of CDC20 in vivo, confirming the in vitro results. In conclusion, this study provides further validation of the potential clinical significance of CDC20 as a strategy to overcome radio-chemoresistance via uncovering a novel role of CDC20 in regulating RPA1 during DNA damage repair.
Flux Calculation for Primary Metabolism Reveals Changes in Allocation of Nitrogen to Different Amino Acid Families When Photorespiratory Activity Changes
Authors: Friedrichs Nils , Shokouhi Danial , Heyer Arnd G.
Published: August 2024

Photorespiration, caused by oxygenation of the enzyme Rubisco, is considered a wasteful process, because it reduces photosynthetic carbon gain, but it also supplies amino acids and is involved in amelioration of stress. Here, we show that a sudden increase in photorespiratory activity not only reduced carbon acquisition and production of sugars and starch, but also affected diurnal dynamics of amino acids not obviously involved in the process. Flux calculations based on diurnal metabolite profiles suggest that export of proline from leaves increases, while aspartate family members accumulate. An immense increase is observed for turnover in the cyclic reaction of glutamine synthetase/glutamine-oxoglutarate aminotransferase (GS/GOGAT), probably because of increased production of ammonium in photorespiration. The hpr1-1 mutant, defective in peroxisomal hydroxypyruvate reductase, shows substantial alterations in flux, leading to a shift from the oxoglutarate to the aspartate family of amino acids. This is coupled to a massive export of asparagine, which may serve in exchange for serine between shoot and root.
Chitosan–Alginate Gels for Sorption of Hazardous Materials: The Effect of Chemical Composition and Physical State
Authors: Fekete Erika , Csiszár Emília
Published: August 2024

Chitosan, alginate, and chitosan–alginate (50:50) mixed hydrogels were prepared by freeze casting, freeze-drying, and subsequent physical cross-linking. Chitosan was cross-linked with citrate and alginate with calcium ions, while the mixed gels were cross-linked with both cross-linking agents. Both cryogels and xerogels were obtained by lyophilization and drying of the hydrogels. We investigated the effect of the chemical composition and the physical state of gels on the gel structure and sorption of model dyes. Alginate and mixed gels cross-linked with Ca2+ ions sorbed 80–95% of cationic dye from the solutions. The chitosan gels are primarily capable of adsorbing anionic dyes, but at near-neutral pH, their capacity is lower than that of alginate gels, showing 50–60% dye sorption. In the case of alginate gels, the dye sorption capacity of xerogels, cryogels, and hydrogels was the same, but for chitosan gels, the hydrogels adsorbed slightly less dye than the dried gels.
An In-Depth Approach to the Associations between MicroRNAs and Viral Load in Patients with Chronic Hepatitis B—A Systematic Review and Meta-Analysis
Authors: Manea Marina , Mărunțelu Ion , Constantinescu Ileana
Published: August 2024

Scientists study the molecular activities of the hepatitis B virus (HBV). However, in vivo experiments are scarce. Some microRNAs are HBV-related, but their exact mechanisms are unknown. Our study provides an up-to-date view of the associations between microRNAs and HBV-DNA levels in chronically infected individuals. We conducted this large-scale research on five databases according to PRISMA guidance. Joanna Briggs Institute tools and Newcastle Ottawa Quality Assessment scores helped with quality evaluations. R 4.2.2 performed statistical computations for the meta-analysis. DIANA-microT 2023 and g:Profiler enriched the predictions of liver genes associated with miR-122 and miR-192-5p. From the 1313 records, we eliminated those irrelevant to our theme, non-article methodologies, non-English entries, and duplicates. We assessed associations between microRNAs and HBV-DNA levels. Overall, the pooled correlations favoured the general idea of the connection between non-coding molecules and viremia levels. MiR-122 and miR-192-5p were the most researched microRNAs, significantly associated with HBV-DNA levels. The connections between miR-122, miR-192-5p, let-7, miR-215, miR-320, and viral loads need further in vivo assessment. To conclude, this study evaluates systematically, for the first time, the correlations between non-coding molecules and viremia levels in patients. Our meta-analysis emphasizes potentially important pathways toward new inhibitors of the viral replication cycle.
Concomitance of Pericardial Tamponade and Pulmonary Embolism in an Invasive Mucinous Lung Adenocarcinoma with Atypical Presentation: Diagnostic and Therapeutic Pitfalls—Case Report and Literature Review
Authors: Bertici Nicoleta Sorina , Tudoran Cristina , Bertici Razvan Adrian , Fira-Mladinescu Ovidiu , Jianu Dragos Catalin , Streian Caius Glad , Staicu Raluca Elisabeta , Manzur Andrei Raul , Lascu Ana
Published: August 2024

The invasive mucinous adenocarcinoma of the lungs (LIMA) is an uncommon histological subtype of the mucinous adenocarcinoma. In this article, we present the case of a patient with a very high cardiovascular risk profile, diagnosed with LIMA, pericardial tamponade due to secondary dissemination, and pulmonary embolism, whose management rouses many challenges. Despite receiving the correct anticoagulant and antiaggregant therapy, our patient developed repeated acute major cardiovascular events leading to a fatal outcome. To gather additional information on LIMA and the above cluster of pathologies, we performed the first research of the international medical literature for scientific articles published in the last eight years on PubMed, ResearchGate, Clarivate, and Google Scholar. As the first literature research failed to identify any case similar to our patient, we performed a second study of the same databases for subjects with lung adenocarcinoma instead of LIMA and the same comorbidities, and we found 10 cases. LIMA is a less frequent type of adenocarcinoma, with polymorphic radiologic appearances on the chest computed tomography, frequently mimicking pneumonia, and thus delaying the diagnosis and therapy. It has a worse prognosis and higher mortality than the common adenocarcinoma, but information on its secondary dissemination and complications is still required.
New Amphiphilic Terpolymers of N-Vinylpyrrolidone with Acrylic Acid and Triethylene Glycol Dimethacrylate as Promising Drug Delivery: Design, Synthesis and Biological Properties In Vitro
Authors: Kurmaz Svetlana V. , Komendant Roman I. , Perepelitsina Evgenia O. , Kurmaz Vladimir A. , Khodos Igor I. , Emelyanova Nina S. , Filatova Natalia V. , Amozova Vera I. , Balakina Anastasia A. , Terentyev Alexey A.
Published: August 2024

The terpolymers of N-vinylpyrrolidone (VP) with acrylic acid and triethylene glycol methacrylate were synthesized with more than 90% yield by radical copolymerization in ethanol from monomeric mixtures of different molar composition (98:2:2, 95:5: 2 and 98:2:5) and their monomer composition, absolute molecular masses and hydrodynamic radii in aqueous media were determined. Using the MTT test, these terpolymers were established to be low toxic for non-tumor Vero cells and HeLa tumor cells. Polymer compositions of hydrophobic dye methyl pheophorbide a (MPP) based on studied terpolymers and linear polyvinylpyrrolidone (PVP) were obtained and characterized in water solution. Quantum-chemical modeling of the MPP-copolymer structures was conducted, and the possibility of hydrogen bond formation between terpolymer units and the MPP molecule was shown. Using fluorescence microscopy, the accumulation and distribution of polymer particles in non-tumor (FetMSC) and tumor (HeLa) cells was studied, and an increase in the accumulation of MPP with both types of particles was found.
Kismet/CHD7/CHD8 and Amyloid Precursor Protein-like Regulate Synaptic Levels of Rab11 at the Drosophila Neuromuscular Junction
Authors: Hendricks Emily L. , Linskey Nicole , Smith Ireland R. , Liebl Faith L. W.
Published: August 2024

The transmembrane protein β-amyloid precursor protein (APP) is central to the pathophysiology of Alzheimer’s disease (AD). The β-amyloid hypothesis posits that aberrant processing of APP forms neurotoxic β-amyloid aggregates, which lead to the cognitive impairments observed in AD. Although numerous additional factors contribute to AD, there is a need to better understand the synaptic function of APP. We have found that Drosophila APP-like (APPL) has both shared and non-shared roles at the synapse with Kismet (Kis), a chromatin helicase binding domain (CHD) protein. Kis is the homolog of CHD7 and CHD8, both of which are implicated in neurodevelopmental disorders including CHARGE Syndrome and autism spectrum disorders, respectively. Loss of function mutations in kis and animals expressing human APP and BACE in their central nervous system show reductions in the glutamate receptor subunit, GluRIIC, the GTPase Rab11, and the bone morphogenetic protein (BMP), pMad, at the Drosophila larval neuromuscular junction (NMJ). Similarly, processes like endocytosis, larval locomotion, and neurotransmission are deficient in these animals. Our pharmacological and epistasis experiments indicate that there is a functional relationship between Kis and APPL, but Kis does not regulate appl expression at the larval NMJ. Instead, Kis likely influences the synaptic localization of APPL, possibly by promoting rab11 transcription. These data identify a potential mechanistic connection between chromatin remodeling proteins and aberrant synaptic function in AD.
Global Realism with Bipolar Strings: From Bell Test to Real-World Causal-Logical Quantum Gravity and Brain-Universe Similarity for Entangled Machine Thinking and Imagination
Authors: Zhang Wen-Ran
Published: August 2024

Following Einstein’s prediction that “Physics constitutes a logical system of thought” and “Nature is the realization of the simplest conceivable mathematical ideas”, this topical review outlines a formal extension of local realism limited by the speed of light to global realism with bipolar strings (GRBS) that unifies the principle of locality with quantum nonlocality. The related literature is critically reviewed to justify GRBS which is shown as a necessary and inevitable consequence of the Bell test and an equilibrium-based axiomatization of physics and quantum information science for brain–universe similarity and human-level intelligence. With definable causality in regularity and mind–light–matter unity for quantum superposition/entanglement, bipolar universal modus ponens (BUMP) in GRBS makes quantum emergence and submergence of spacetime logically ubiquitous in both the physical and mental worlds—an unexpected but long-sought simplification of quantum gravity with complete background independence. It is shown that GRBS forms a basis for quantum intelligence (QI)—a spacetime transcendent, quantum–digital compatible, analytical quantum computing paradigm where bipolar strings lead to bipolar entropy as a nonlinear bipolar dynamic and set–theoretic unification of order and disorder as well as linearity and nonlinearity for energy/information conservation, regeneration, and degeneration toward quantum cognition and quantum biology (QCQB) as well as information-conservational blackhole keypad compression and big bang data recovery. Subsequently, GRBS is justified as a real-world quantum gravity (RWQG) theory—a bipolar relativistic causal–logical reconceptualization and unification of string theory, loop quantum gravity, and M-theory—the three roads to quantum gravity. Based on GRBS, the following is posited: (1) life is a living bipolar superstring regulated by bipolar entropy; (2) thinking with consciousness and memory growth as a prerequisite for human-level intelligence is fundamentally mind–light–matter unitary QI logically equivalent to quantum emergence (entanglement) and submergence (collapse) of spacetime. These two posits lead to a positive answer to the question “If AI machine cannot think, can QI machine think?”. Causal–logical brain modeling (CLBM) for entangled machine thinking and imagination (EMTI) is proposed and graphically illustrated. The testability and falsifiability of GRBS are discussed.
Maximal LoRa Range for Unmanned Aerial Vehicle Fleet Service in Different Environmental Conditions
Authors: Felli Lorenzo , Giuliano Romeo , De Negri Andrea , Terlizzi Francesco , Mazzenga Franco , Vizzarri Alessandro
Published: August 2024

This study investigates communication between UAVs using long range (LoRa) devices, focusing on the interaction between a LoRa gateway UAV and other UAVs equipped with LoRa transmitters. By conducting experiments across various geographical regions, this study aims to delineate the fundamental boundary conditions for the efficient control of a UAV fleet. The parameters under analysis encompass inter-device spacing, radio interference effects, and terrain topography. This research yields pivotal insights into communication network design and optimization, thereby enhancing operational efficiency and safety within diverse geographical contexts for UAV operations. Further research insights could involve a weather analysis and implementation of improved solutions in terms of communication systems.