bluuebunny/crossref_metadata_2025 huggingface dataset:
Motorcycle Ownership Assessment and Estimation in Makurdi, North Central, Nigeria: A Multinomial Logit Model Approach
Authors: Gbagir K. , Etu2 J. E. , Oyedepo O. J.
Published: March 2019

With the rapid rise in problems associated with use of motorcycles as alternative means to inadequate public transportation, this study seeks to identify household factors influencing motorcycle ownership in Makurdi. The study estimates the influence of the various household factors identified and a model for predicting motorcycle ownership is developed for the study area. Data were collected via a questionnaire survey of 1412 households in the study area. The survey revealed that the number of motorcycles owned per household in the low density zone was 0.67 while that for the medium and high density zones was 0.62 and 0.79 respectively. The multinomial logit model developed predicted that 67% of households owned motorcycles as compared to 71% observed from survey data.  Severity applications of the model to test the effects of changing economic situations on motorcycle ownership showed that residents of the study area are more disposed to owning motorcycles in periods of recession than periods of economic boom. The study gives an understanding of motorcycle growth pattern and ownership characteristics in the study area and will therefore serve as a relevant input for planning, regulation and control of motorcycle activities in the study area.
Sowing Date Affects Maize Development and Yield in Irrigated Mediterranean Environments
Authors: Maresma Angel , Ballesta Astrid , Santiveri Francisca , Lloveras Jaume
Published: March 2019

Timely sowing is critical for maximizing yield for both grain and biomass in maize. The effects of early (mid-March), normal (mid-April), and late (mid-May) sowing date (SD) were studied over a three-year period in irrigated maize under Mediterranean conditions. Early SD increased the number of days from sowing to plant emergence. Late SD reduced the number of days to plant maturity, and had higher forage yields, higher grain humidity, and taller plants. The average grain and forage yields achieved were 13.2 and 21.3 Mg ha−1; 14.0 and 25.1 Mg ha−1; and 12.8 and 27.6 Mg ha−1, for crops with early, normal, and late SD, respectively. The data support the general perception of farmers that April sowings are the most appropriate in the area where the experiments were carried out. Early SD resulted in lower population densities, while later SD did not yield (grain) as high. However, late SD produced taller plants that contributed to achieve higher forage yields. Late SD could be interesting for double annual forage cropping systems. Sowing at the most appropriate time, when the soil is warm, ensures a good level of maize grain production. Future research could focus in the effect of SD for total annual yields in double-annual cropping systems.
In Vitro Antioxidant and Antimicrobial Properties of Flower, Leaf, and Stem Extracts of Korean Mint
Authors: Park Chang Ha , Yeo Hyeon Ji , Baskar Thanislas Bastin , Park Ye Eun , Park Jong Seok , Lee Sook Young , Park Sang Un
Published: March 2019

Traditionally, Agastache rugosa (Korean mint) has been widely used to treat various infectious diseases. The aims of this study were to: (i) determine the phenylpropanoid content of the plant using high-performance liquid chromatography; (ii) undertake total anthocyanin, flavonoid, and phenolic assays; (iii) and evaluate the antioxidant and antibacterial properties of the methanol extracts from the stem, leaves, and flowers of Korean mint. The total anthocyanin, flavonoid, and phenolic content assays showed that the flowers had higher phenolic levels than the stem and leaves. The reducing power, the 2,2-diphenyl-1-picrylhydrazyl superoxide radical scavenging abilities, and the hydrogen peroxide radical scavenging activities were also evaluated so that the antioxidant activities of the extracts from the different plant parts could be evaluated. The flower extracts revealed higher antioxidant properties than the other parts. The antibacterial properties of the methanol extracts from A. rugosa were analyzed by the disc diffusion method, and the flower extracts had higher antibacterial activities against the six bacterial strains used in the study than the other parts. This study provides information on the synergistic antioxidant and antibacterial properties of phenolics derived from the different parts of Korean mint.
Development and Validation of an Analytical Method for Carnosol, Carnosic Acid and Rosmarinic Acid in Food Matrices and Evaluation of the Antioxidant Activity of Rosemary Extract as a Food Additive
Authors: Choi Seung-Hyun , Jang Gill-Woong , Choi Sun-Il , Jung Tae-Dong , Cho Bong-Yeon , Sim Wan-Sup , Han Xionggao , Lee Jin-Sol , Kim Do-Yeon , Kim Dan-Bi , Lee Ok-Hwan
Published: March 2019

Antioxidants are used to prevent the oxidation of foods. When used for food additive purposes, the dosage should be regulated and the functionality evaluated to ensure stability. In this study, we performed a method validation for the quantitative analysis of rosemary extract residues and evaluated the antioxidant activity of rosemary extract in food matrices. The validated method was able to determine rosemary extract under the optimized high-performance liquid chromatography-photodiode array (HPLC-PDA) conditions. Furthermore, the antioxidant activity was evaluated by peroxide value, acid value, and in terms of the residual antioxidant levels in lard oil. For HPLC-PDA analysis, the limit of detection and quantification of rosemary extracts was ranged from 0.22 to 1.73 μg/mL, 0.66 to 5.23 μg/mL and the recoveries of the rosemary extracts ranged from 70.6 to 114.0%, with relative standard deviations of between 0.2% and 3.8%. In terms of antioxidant activity, carnosic acid performed better than carnosol. Furthermore, by evaluation of the residual antioxidant level using HPLC, we found that carnosic acid is more stable in lard oil than carnosol. These results indicate that rosemary extract can be used as an antioxidant and that the analytical method is suitable for the determination of rosemary extract in various food samples.
Active Noise Control over Space: A Subspace Method for Performance Analysis
Authors: Zhang Jihui , Abhayapala Thushara D. , Zhang Wen , Samarasinghe Prasanga N.
Published: March 2019

In this paper, we investigate the maximum active noise control performance over a three-dimensional (3-D) spatial space, for a given set of secondary sources in a particular environment. We first formulate the spatial active noise control (ANC) problem in a 3-D room. Then we discuss a wave-domain least squares method by matching the secondary noise field to the primary noise field in the wave domain. Furthermore, we extract the subspace from wave-domain coefficients of the secondary paths and propose a subspace method by matching the secondary noise field to the projection of primary noise field in the subspace. Simulation results demonstrate the effectiveness of the proposed algorithms by comparison between the wave-domain least squares method and the subspace method, more specifically the energy of the loudspeaker driving signals, noise reduction inside the region, and residual noise field outside the region. We also investigate the ANC performance under different loudspeaker configurations and noise source positions.
Numerical Study of a Proposed Semi-Submersible Floating Platform with Different Numbers of Offset Columns Based on the DeepCwind Prototype for Improving the Wave-Resistance Ability
Authors: Liu Zhenqing , Fan Yicheng , Wang Wei , Qian Guowei
Published: March 2019

DeepCwind semi-submersible floating offshore wind turbines have been widely examined, and in some countries this type of floating offshore wind turbine has been adopted in the construction of floating wind farms. However, the DeepCwind semi-submersible floating offshore wind turbines still experience large surge motion that limits their operational time. Therefore, in this study, a semi-submersible floating platform with different numbers of offset columns, but with the same total weight, based on the DeepCwind prototype is proposed. From the free-decay test, it was found that the number of the floating columns will affect the natural frequency of the platform. Furthermore, the regular wave test in the time domain and the irregular wave test in the frequency domain show that increasing the number of the floating columns will reduce the surge motion greatly, while the effects in the heave and pitch motions are not obvious.
Effect of Process Parameters on the Generated Surface Roughness of Down-Facing Surfaces in Selective Laser Melting
Authors: Charles Amal , Elkaseer Ahmed , Thijs Lore , Hagenmeyer Veit , Scholz Steffen
Published: March 2019

Additive manufacturing provides a number of benefits in terms of infinite freedom to design complex parts and reduced lead-times while globally reducing the size of supply chains as it brings all production processes under one roof. However, additive manufacturing (AM) lags far behind conventional manufacturing in terms of surface quality. This proves a hindrance for many companies considering investment in AM. The aim of this work is to investigate the effect of varying process parameters on the resultant roughness of the down-facing surfaces in selective laser melting (SLM). A systematic experimental study was carried out and the effects of the interaction of the different parameters and their effect on the surface roughness (Sa) were analyzed. It was found that the interaction and interdependency between parameters were of greatest significance to the obtainable surface roughness, though their effects vary greatly depending on the applied levels. This behavior was mainly attributed to the difference in energy absorbed by the powder. Predictive process models for optimization of process parameters for minimizing the obtained Sa in 45° and 35° down-facing surface, individually, were achieved with average error percentages of 5% and 6.3%, respectively, however further investigation is still warranted.
Experimental Study on a Prefabricated Lightweight Concrete-Filled Steel Tubular Framework Composite Slab Structure Subjected to Reversed Cyclic Loading
Authors: Suizi Jia , Wanlin Cao , Zibin Liu , Wei Ding , Yingnan Su
Published: March 2019

A building structure comprising a prefabricated lightweight concrete-filled steel tubular (CFST) framework composite slab structure is proposed. Five full-scale specimens (i.e., one empty framework and four-walled frameworks) were tested under reversed cyclic loading to study their earthquake-resistance performance. Of the four wall specimens, three were walled using composite slabs, one had no openings, one had a window opening, and one had a door opening. One was walled with a concealed steel-truss slab. A comparative study on the strength, stiffness, ductility, hysteresis characteristics, and dissipated energy of the specimens was performed. The working mechanism of the framework and slab was then analyzed. The results show that, if reasonably assembled and connected, the framework and slab work in a well-coordinated manner. The walled framework had greater lateral load-bearing capacity, better energy-dissipation, greater stiffness reduction, and better deformability than an empty framework. The area and type of slab opening had a significant impact on structural performance because a door or window opening contributed to a smaller lateral load-bearing capacity and initial secant stiffness of the structure. However, this had no clear impact on the accumulative dissipated energy of the structure.
Observation to Building Thermal Characteristic of Green Façade Model Based on Various Leaves Covered Area
Authors: Widiastuti Ratih , Caesarendra Wahyu , Zaini Juliana
Published: March 2019

This project is part of research series to observe the thermal characteristic of green facade to minimize the cooling load inside building. The aim is to analyze the building thermal characteristic on the green facade installed on a building model. Various kinds of leaves covered area (experiment I: 0%, experiment II: 50%, and experiment III: 90%) were used as the main parameter. Calculation in decrement factor and time lag were also done in order to support the analysis of heat flows calculations. Data measurement showed that ambient and facade surface of green facade with the greatest leaves covered area (experiment III) had lower temperature profiles. Data calculation also showed the highest of average heat flows were found out in the bare wall model. Results for heat flow in the wall surface were 4.69 W/m2 (experiment I), 3.88 W/m2 (experiment II), and 1.61 W/m2 (experiment III). While for heat flows through indoor air space, they were 27.75 W/m2 (experiment I), −5.10 W/m2 (experiment II), and 8.99 W/m2 (experiment III). As shown from data analysis, the quantities of leaves covered area effected the cooling down on the building envelope by reducing the quantity of heat flows from exterior to interior side.
Overactive IGF1/Insulin Receptors and NRASQ61R Mutation Drive Mechanisms of Resistance to Pazopanib and Define Rational Combination Strategies to Treat Synovial Sarcoma
Authors: Lanzi Cinzia , Dal Bo Laura , Favini Enrica , Tortoreto Monica , Beretta Giovanni Luca , Arrighetti Noemi , Zaffaroni Nadia , Cassinelli Giuliana
Published: March 2019

Pazopanib is approved for treatment of advanced soft tissue sarcomas, but primary and secondary drug resistance limits its clinical utility. We investigated the molecular mechanisms mediating pazopanib resistance in human synovial sarcoma (SS) models. We found reduced cell sensitivity to pazopanib associated with inefficient inhibition of the two critical signaling nodes, AKT and ERKs, despite strong inhibition of the main drug target, PDGFRα. In the CME-1 cell line, overactivation of IGF1 and Insulin receptors (IGF1R/InsR) sustained AKT activation and pazopanib resistance, which was overcome by a combination treatment with the double IGF1R/InsR inhibitor BMS754807. In the highly pazopanib resistant MoJo cell line, NRASQ61R mutation sustained constitutive ERK activation. Transfection of the NRAS mutant in the pazopanib sensitive SYO-1 cell line increased the drug IC50. MoJo cells treatment with pazopanib in combination with the MEK inhibitor trametinib restored ERK inhibition, synergistically inhibited cell growth, and induced apoptosis. The combination significantly enhanced the antitumor efficacy against MoJo orthotopic xenograft abrogating growth in 38% of mice. These findings identified two different mechanisms of intrinsic pazopanib resistance in SS cells, supporting molecular/immunohistochemical profiling of tumor specimens as a valuable approach to selecting patients who may benefit from rational drug combinations.
Dysregulation of Macropinocytosis Processes in Glioblastomas May Be Exploited to Increase Intracellular Anti-Cancer Drug Levels: The Example of Temozolomide
Authors: Colin Margaux , Delporte Cédric , Janky Rekin’s , Lechon Anne-Sophie , Renard Gwendoline , Van Antwerpen Pierre , Maltese William A. , Mathieu Véronique
Published: March 2019

Macropinocytosis is a clathrin-independent endocytosis of extracellular fluid that may contribute to cancer aggressiveness through nutrient supply, recycling of plasma membrane and receptors, and exosome internalization. Macropinocytosis may be notably triggered by epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor (PDGFR), two well-known markers for glioblastoma aggressiveness. Therefore, we studied whether the expression of key actors of macropinocytosis is modified in human glioma datasets. Strong deregulation has been evidenced at the mRNA level according to the grade of the tumor, and 38 macropinocytosis-related gene signatures allowed discrimination of the glioblastoma (GBM) samples. Honokiol-induced vacuolization was then compared to vacquinol-1 and MOMIPP, two known macropinocytosis inducers. Despite high phase-contrast morphological similarities, honokiol-induced vacuoles appeared to originate from both endocytosis and ER. Also, acridine orange staining suggested differences in the macropinosomes’ fate: their fusion with lysosomes appeared very limited in 3-(5-methoxy -2-methyl-1H-indol-3-yl)-1-(4-pyridinyl)-2-propen-1-one (MOMIPP)-treated cells. Nevertheless, each of the compounds markedly increased temozolomide uptake by glioma cells, as evidenced by LC-MS. In conclusion, the observed deregulation of macropinocytosis in GBM makes them prone to respond to various compounds affecting their formation and/or intracellular fate. Considering that sustained macropinocytosis may also trigger cell death of both sensitive and resistant GBM cells, we propose to envisage macropinocytosis inducers in combination approaches to obtain dual benefits: increased drug uptake and additive/synergistic effects.
Engagement with tNOX (ENOX2) to Inhibit SIRT1 and Activate p53-Dependent and -Independent Apoptotic Pathways by Novel 4,11-Diaminoanthra[2,3-b]furan-5,10-diones in Hepatocellular Carcinoma Cells
Authors: Lin Chia-Yang , Islam Atikul , Su Claire J. , Tikhomirov Alexander S. , Shchekotikhin Andrey E. , Chuang Show-Mei , Chueh Pin Ju , Chen Yao Li
Published: March 2019

Hepatocellular carcinoma (HCC) is the most frequent primary malignancy of the liver and is among the top three causes of cancer-associated death worldwide. However, the clinical use of chemotherapy for HCC has been limited by various challenges, emphasizing the urgent need for novel agents with improved anticancer properties. We recently synthesized and characterized a series of 4,11-diaminoanthra[2,3-b]furan-5,10-dione derivatives that exhibit potent apoptotic activity against an array of cancer cell lines, including variants with multidrug resistance. Their effect on liver cancer cells, however, was unknown. Here, we investigated three selected 4,11-diaminoanthra[2,3-b]furan-5,10-dione derivatives (compounds 1–3) for their cytotoxicity and the underlying molecular mechanisms in wild-type or p53-deficient HCC cells. Cytotoxicity was determined by WST-1 assays and cell impedance measurements and apoptosis was analyzed by flow cytometry. The interaction between compounds and tumor-associated NADH oxidase (tNOX, ENOX2) was studied by cellular thermal shift assay (CETSA). We found that compound 1 and 2 induced significant cytotoxicity in both HepG2 and Hep3B lines. CETSA revealed that compounds 1 and 2 directly engaged with tNOX, leading to a decrease in the cellular NAD+/NADH ratio. This decreased the NAD+-dependent activity of Sirtuin 1 (SIRT1) deacetylase. In p53-wild-type HepG2 cells, p53 acetylation/activation was enhanced, possibly due to the reduction in SIRT1 activity, and apoptosis was observed. In p53-deficient Hep3B cells, the reduction in SIRT1 activity increased the acetylation of c-Myc, thereby reactivating the TRAIL pathway and, ultimately leading to apoptosis. These compounds thus trigger apoptosis in both cell types, but via different pathways. Taken together, our data show that derivatives 1 and 2 of 4,11-diaminoanthra[2,3-b]furan-5,10-diones engage with tNOX and inhibit its oxidase activity. This results in cytotoxicity via apoptosis through tNOX-SIRT1 axis to enhance the acetylation of p53 or c-Myc in HCC cells, depending on their p53 status.
Follow-Up Liver Stiffness Measurements after Liver Resection Influence Oncologic Outcomes of Hepatitis-B-Associated Hepatocellular Carcinoma with Liver Cirrhosis
Authors: Lee Jung Il , Lee Hyun Woong , Kim Seung Up , Ahn Sang Hoon , Lee Kwan Sik
Published: March 2019

The severity of liver fibrosis can be noninvasively evaluated by measuring liver stiffness (LS) using transient elastography. This study aimed to evaluate the prognostic value of achieving low liver stiffness measurement (LSM) in patients with cirrhosis confirmed from the resected liver due to hepatocellular carcinoma (HCC). A total of 184 patients that received curative surgery for HCC related to the hepatitis B virus at Barcelona Clinic Liver Cancer stage 0–A, and had a METAVIR fibrosis score of 4 were investigated. LSM significantly decreased after antiviral therapy during follow-up (p = 0.001), and achieving LSM ≤8 kilopascal (kPa) suggested a reduced risk of late recurrence (>12 months) (hazard ratio (HR), 0.519; 95% confidence interval (CI), 0.307–0.877; p = 0.014). Older age at surgery (≥45 years) and multiple HCC nodules predicted an increased risk of late recurrence (HR, 3.270; 95% CI, 1.296–8.251; p = 0.012; and HR, 3.146; 95% CI, 1.396–7.089; p = 0.006). Decreased LSM also suggested decreased mortality (HR, 0.251; 95% CI, 0.086–0.756; p = 0.045) along with baseline low aspartate aminotransferase-to-platelet ratio index (APRI) score (<1.5) (HR, 0.251; 95% CI, 0.086–0.759; p = 0.041). Having early HCC recurrence (HR, 9.416; 95% CI, 3.566–24.861; p < 0.001) and microvascular tumor invasion (HR, 3.191; 95% CI, 1.188–8.568; p = 0.021) predicted increased mortality. Among HCC patients with liver cirrhosis under antiviral therapy, achieving low LSM (≤8 kPa) predicted reduced late HCC recurrence.
N-Glycomic and Transcriptomic Changes Associated with CDX1 mRNA Expression in Colorectal Cancer Cell Lines
Authors: Holst Stephanie , Wilding Jennifer L. , Koprowska Kamila , Rombouts Yoann , Wuhrer Manfred
Published: March 2019

The caudal-related homeobox protein 1 (CDX1) is a transcription factor, which is important in the development, differentiation, and homeostasis of the gut. Although the involvement of CDX genes in the regulation of the expression levels of a few glycosyltransferases has been shown, associations between glycosylation phenotypes and CDX1 mRNA expression have hitherto not been well studied. Triggered by our previous study, we here characterized the N-glycomic phenotype of 16 colon cancer cell lines, selected for their differential CDX1 mRNA expression levels. We found that high CDX1 mRNA expression associated with a higher degree of multi-fucosylation on N-glycans, which is in line with our previous results and was supported by up-regulated gene expression of fucosyltransferases involved in antenna fucosylation. Interestingly, hepatocyte nuclear factors (HNF)4A and HNF1A were, among others, positively associated with high CDX1 mRNA expression and have been previously proven to regulate antenna fucosylation. Besides fucosylation, we found that high CDX1 mRNA expression in cancer cell lines also associated with low levels of sialylation and galactosylation and high levels of bisection on N-glycans. Altogether, our data highlight a possible role of CDX1 in altering the N-glycosylation of colorectal cancer cells, which is a hallmark of tumor development.
CD44s Assembles Hyaluronan Coat on Filopodia and Extracellular Vesicles and Induces Tumorigenicity of MKN74 Gastric Carcinoma Cells
Authors: Härkönen Kai , Oikari Sanna , Kyykallio Heikki , Capra Janne , Hakkola Sini , Ketola Kirsi , Thanigai Arasu Uma , Daaboul George , Malloy Andrew , Oliveira Carla , Jokelainen Otto , Sironen Reijo , Hartikainen Jaana M. , Rilla Kirsi
Published: March 2019

CD44 is a multifunctional adhesion molecule typically upregulated in malignant, inflamed and injured tissues. Due to its ability to bind multiple ligands present in the tumor microenvironment, it promotes multiple cellular functions related to tumorigenesis. Recent data has shown that CD44 and its principal ligand hyaluronan (HA) are carried by extracellular vesicles (EV) derived from stem and tumor cells, but the role of CD44 in EV shedding has not been studied so far. To answer this question, we utilized CD44-negative human gastric carcinoma cell line MKN74 manipulated to stably express CD44 standard form (CD44s). The effect of CD44s expression on HA metabolism, EV secretion, morphology and growth of these cells was studied. Interestingly, HAS2 and HYAL2 expression levels were significantly upregulated in CD44s-expressing cells. Cell-associated HA levels were significantly increased, while HA levels in the culture medium of CD44s-positive cells was lower compared to CD44s-negative MOCK cells. CD44s expression had no significant effect on the proliferation capacity of cells, but cells showed diminished contact inhibition. Superresolution imaging revealed that CD44s and HA were accumulated on filopodia and EVs secreted from CD44s-positive cells, but no differences in total numbers of secreted EV between CD44s-negative and -positive cells was detected. In 3D cultures, CD44s-expressing cells had an enhanced invasion capacity in BME gel and increased spheroidal growth when cultured in collagen I gel. No significant differences in mitotic activity, tumor size or morphology were detected in CAM assays. However, a significant increase in HA staining coverage was detected in CD44s-positive tumors. Interestingly, CD44s-positive EVs embedded in HA-rich matrix were detected in the stromal areas of tumors. The results indicate that CD44s expression significantly increases the HA binding capacity of gastric cancer cells, while the secreted HA is downregulated. CD44s is also carried by EVs secreted by CD44s-expressing cells. These findings highlight the potential usefulness of CD44s and its ligands as multipurpose EV biomarkers, because they are upregulated in inflammatory, injured, and cancer cells and accumulate on the surface of EVs secreted in these situations.
Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft
Authors: Gentile Pietro , Piccinno Maria Serena , Calabrese Claudio
Published: March 2019

Human adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion. For this study, we undertook a histological evaluation of traditional fat graft compared with fat graft enriched with stromal vascular fraction cells isolated by the Celution™ system to assess the interactions between cells and adipose tissue before the breast injection. In addition, we reported on histological analyses of biopsies derived from fat grafted (traditional or enriched with SVFs) in the breast in order to assess the quality of the adipose tissue, fibrosis and vessels. The hASCs derived from enzymatic digestion were systematically characterized for growth features, phenotype and multi-potent differentiation potential. They fulfill the definition of mesenchymal stem cells, albeit with a higher neural phenotype profile. These cells also express genes that constitute the core circuitry of self-renewal such as OCT4, SOX2, NANOG and neurogenic lineage genes such as NEUROD1, PAX6 and SOX3. Such findings support the hypothesis that hASCs may have a potential usefulness in neurodegenerative conditions. These data can be helpful for the development of new therapeutic approaches in personalized medicine to assess safety and efficacy of the breast reconstruction.
Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples
Authors: Lukyanenko Kirill , Denisov Ivan , Sorokin Vladimir , Yakimov Anton , Esimbekova Elena , Belobrov Peter
Published: March 2019

Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.
Using the School Environment to Promote Walking amongst Adolescent Females: A Mixed-Method Study
Authors: Carlin Angela , Murphy Marie H. , Gallagher Alison M.
Published: March 2019

Schools have the potential to promote physical activity (PA) in adolescents through physical education (PE) and extra-curricular PA. The aims of this study were to firstly understand the experiences of adolescent females who participated in a school-based walking programme (the Walking In ScHools (WISH) study) and secondly, to assess the potential for schools to further promote PA outside of structured PE. A sample of female participants (n = 45, mean age 13.1 years) who participated in the WISH study were randomly selected to participate in focus group discussions, to explore their experiences of the intervention. In addition, an online survey was distributed to all post-primary schools (n = 208) in Northern Ireland to assess the provision of extra-curricular PA and further evaluate the feasibility of the WISH study. In total, six focus groups were conducted. Walking during the school day was viewed as an acceptable form of PA by adolescent females, providing an opportunity to be active with friends, and helped participants overcome barriers previously associated with being active at school. Responding schools (n = 59) identified adolescent females and non-sporty pupils as sub-groups who would benefit most from participation in a school-based walking programme. This study has highlighted that the delivery of a walking programme within the school setting is acceptable, warranted and practically feasible from the point of view of adolescent females and key stakeholders within the school setting.
Improved Adhesion of TiAlSiN Nanocomposite Coatings on Cemented Carbide Substrate by Pre-Implantation
Authors: Wang Lei , Li Liuhe , Li Guodong , Ma Quansheng
Published: March 2019

TiAlSiN coatings were deposited on YT 15 cemented carbide substrate by reactive direct current magnetron sputtering (DCMS) in a Plasma Immersion Ion Implantation and Deposition (PIII&D) system. The pre-implantation step and the coating deposition were carried out in the same experimental facility. In this article the effects of pre-implantation of several different elements (N, C, and O) were investigated. The adhesion strength, hardness, micro-structure, element concentration, depth profile, and the formation of coatings after the PIII experiments were characterized by a wide variety of techniques such as Rockwell indentation, scratch test, nano-indentation measurement, X-ray diffraction, energy dispersive spectroscopy, and Auger electron spectroscopy. The results showed that the adhesive strength of TiAlSiN coatings was significantly improved on samples pre-implanted with N and O whereas only slightly improved with pre-implantation of C. Additionally, the microstructure and mechanical properties of the TiAlSiN coatings were also altered through pre-implantation. The improved adhesion could be explained by the grain refinement and surface energy enhancement of the substrate by pre-implantation.
Release of Pharmaceutical Peptides in an Aggregated State: Using Fibrillar Polymorphism to Modulate Release Levels
Authors: Madsen Jens K. , Christiansen Gunna , Giehm Lise , Otzen Daniel E.
Published: March 2019

Traditional approaches to achieve sustained delivery of pharmaceutical peptides traditionally use co-excipients (e.g., microspheres and hydrogels). Here, we investigate the release of an amyloidogenic glucagon analogue (3474) from an aggregated state and the influence of surfactants on this process. The formulation of peptide 3474 in dodecyl maltoside (DDM), rhamnolipid (RL), and sophorolipid (SL) led to faster fibrillation. When the aggregates were subjected to multiple cycles of release by repeated resuspension in fresh buffer, the kinetics of the release of soluble peptide 3474 from different surfactant aggregates all followed a simple exponential decay fit, with half-lives of 5–18 min and relatively constant levels of release in each cycle. However, different amounts of peptide are released from different aggregates, ranging from 0.015 mg/mL (3475-buffer) up to 0.03 mg/mL (3474-DDM), with 3474-buffer and 3474-RL in between. In addition to higher release levels, 3474-DDM aggregates showed a different amyloid FTIR structure, compared to 3474-RL and 3474-SL aggregates and a faster rate of degradation by proteinase K. This demonstrates that the stability of organized peptide aggregates can be modulated to achieve differences in release of soluble peptides, thus coupling aggregate polymorphism to differential release profiles. We achieved aggregate polymorphism by the addition of different surfactants, but polymorphism may also be reached through other approaches, including different excipients as well as changes in pH and salinity, providing a versatile handle to control release profiles.
Thermal Prediction of Convective-Radiative Porous Fin Heatsink of Functionally Graded Material Using Adomian Decomposition Method
Authors: Oguntala George , Sobamowo Gbeminiyi , Ahmed Yinusa , Abd-Alhameed Raed
Published: March 2019

In recent times, the subject of effective cooling have become an interesting research topic for electronic and mechanical engineers due to the increased miniaturization trend in modern electronic systems. However, fins are useful for cooling various low and high power electronic systems. For improved thermal management of electronic systems, porous fins of functionally graded materials (FGM) have been identified as a viable candidate to enhance cooling. The present study presents an analysis of a convective–radiative porous fin of FGM. For theoretical investigations, the thermal property of the functionally graded material is assumed to follow linear and power-law functions. In this study, we investigated the effects of inhomogeneity index of FGM, convective and radiative variables on the thermal performance of the porous heatsink. The results of the present study show that an increase in the inhomogeneity index of FGM, convective and radiative parameter improves fin efficiency. Moreover, the rate of heat transfer in longitudinal FGM fin increases as β increases. The temperature prediction using the Adomian decomposition method is in excellent agreement with other analytical and method.
Effect of High-Density Nanoparticles on Recrystallization and Texture Evolution in Ferritic Alloys
Authors: Aydogan Eda , Rietema Connor J. , Carvajal-Nunez Ursula , Vogel Sven C. , Li Meimei , Maloy Stuart A.
Published: March 2019

Ferritic alloys are important for nuclear reactor applications due to their microstructural stability, corrosion resistance, and favorable mechanical properties. Nanostructured ferritic alloys having a high density of Y-Ti-O rich nano-oxides (NOs < 5 nm) are found to be extremely stable at high temperatures up to ~1100 °C. This study serves to understand the effect of a high density of nano-particles on texture evolution and recrystallization mechanisms in ferritic alloys of 14YWT (14Cr-3W-0.4Ti-0.21Y-Fe wt %) having a high density of nano-particles and dispersion-free FeCrAl (13Cr-5.2Al-0.05Y-2Mo-0.2Si-1Nb wt %). In order to investigate the recrystallization mechanisms in these alloys, neutron diffraction, electron backscattered diffraction, and in situ and ex situ transmission electron microscopy have been utilized. It has been observed that even though the deformation textures of both the 14YWT and FeCrAl alloys evolved similarly, resulting in either the formation (in FeCrAl alloy) or increase (in 14YWT) in γ-fiber texture, the texture evolution during recrystallization is different. While FeCrAl alloy keeps its γ-fiber texture after recrystallization, 14YWT samples develop a ε-fiber as a result of annealing at 1100 °C, which can be attributed to the existence of NOs. In situ transmission electron microscopy annealing experiments on 14YWT show the combination and growth of the lamellar grains rather than nucleation; however, the recrystallization and growth kinetics are slower due to NOs compared to FeCrAl.
Deep Etched Gallium Nitride Waveguide for Raman Spectroscopic Applications
Authors: Hsieh Yu-Li , Chen Wen-Shao , Chang Liann-Be , Chow Lee , Borges Samuel , Schulte Alfons , Huang Shiang-Fu , Jeng Ming-Jer , Yu Chih-Jen
Published: March 2019

Gallium nitride (GaN) materials with a high chemical stability and biocompatibility are well suited for bio-sensing applications and evanescent wave spectroscopy. However, GaN poses challenges for processing, especially for deep etching using conventional etching techniques. Here, we present a dry-etching technique using tetraethyl orthosilicate (TEOS) oxide as an etching barrier. We demonstrate that a sharp, vertically-etched waveguide pattern can be obtained with low surface roughness. The fabricated GaN waveguide structure is further characterized using field-emission scanning electron microscopy, Raman spectroscopy, and a stylus profilometer.
Prospective of (BaCa)(ZrTi)O3 Lead-free Piezoelectric Ceramics
Authors: Liu Wenfeng , Cheng Lu , Li Shengtao
Published: March 2019

Piezoelectric ceramics is a functional material that can convert mechanical energy into electrical energy and vice versa. It can find wide applications ranging from our daily life to high-end techniques and dominates a billion-dollar market. For half a century, the working horse of the field has been the polycrystalline PbZr1−xTixO3 (PZT), which is now globally resisted for containing the toxic element lead. In 2009, our group discovered a non-Pb piezoelectric material, (BaCa)(ZrTi)O3 ceramics (BZT-BCT), which exhibits an ultrahigh piezoelectric coefficient d33 of 560–620 pC/N. This result brought extensive interest in the research field and important consequences for the piezoelectric industry that has relied on PZT. In the present paper, we review the recent progress, both experimental and theoretical, in the BZT-BCT ceramics.
Dirt, Saliva and Leprosy: Anti-Inflammatory and Anti-Infectious Effects
Authors: Oz Helieh S.
Published: March 2019

Ancient Egyptians smeared a mixture of dark soil on their eyelids and believed it protected eyes from unknown forces (illness). Recent studies have proven that the dark soil across the Nile River is rich in natural compounds including lead sulfide, which in low levels, promotes the production of nitric oxide (240-fold) by keratinocytes, with strong immune stimulatory and antimicrobial properties. Current investigations reveal anti-inflammatory and anti-infectious activities—including cytokines and chemokines—in saliva, as well as its friendly microbiota, which lines the surface of the oral cavity, its protection against inflammatory and infectious organisms in the stoma and other organs, such as the cardiovascular and central nervous systems. In fact, saliva may soon become a safe and practical surrogate biomarker for genomic/proteomic evaluations and to replace painful blood drawing and its side effects. Another example is leprosy, or Hansen’s disease, a chronic inflammatory syndrome and neglected tropical disease, which affects the skin, and peripheral and trigeminal neurons causing a lack of sensation to heat and cold and loss of extremities. Leprosy has horrified humans for over 2000 years, as lepers were considered unclean sinners and were subsequently drawn out of towns. This communication scrutinizes the past and the present state of saliva and leprosy to encounter possible mystery and/or wisdom in ancient healing as the mixture of “sputum and dirt” as reported in the biblical time.
A Lightweight, Robust Exploitation System for Temporal Stacks of UAS Data: Use Case for Forward-Deployed Military or Emergency Responders
Authors: Marx Andrew , Chou Yu-Hsi , Mercy Kevin , Windisch Richard
Published: March 2019

The availability and precision of unmanned aerial systems (UAS) permit the repeated collection of very-high quality three-dimensional (3D) data to monitor high-interest areas, such as dams, urban areas, or erosion-prone coastlines. However, challenges exist in the temporal analysis of this data, specifically in conducting change-detection analysis on the high-quality point cloud data. These files are very large in size and contain points in varying locations that do not align between scenes. These large file sizes also limit the use of this data for individuals with low computational resources, such as first responders or forward-deployed soldiers. In response, this manuscript presents an approach that aggregates data spatially into voxels to provide the user with a lightweight, web-based exploitation system coupled with a flexible backend database. The system creates a robust set of tools to analyze large temporal stacks of 3D data and reduces data size by 78%, all while being able to query the original point cloud data. This approach offers a solution for organizations analyzing high-resolution, temporal point-clouds, as well as a possible solution for operations in areas with poor computational and connectivity resources requiring high-quality, 3D data for decision support and planning.
Towards Airborne Thermography via Low-Cost Thermopile Infrared Sensors
Authors: Valente João , Roldán Juan Jesús , Garzón Mario , Barrientos Antonio
Published: March 2019

This paper presents a novel tool capable of collecting thermal signatures inside a building by using low-cost IR temperature sensors mounted on-board an aerial platform. The proposed system aims to facilitate the detection of heat loss inside buildings, which is a key aspect for improving energy efficiency in large commercial or industrial buildings. Current detection systems usually require manual labor as well as the use of expensive instrumentation. The proposed system on the other hand, relies on the use of a small unmanned aerial vehicle carrying low-cost thermopile IR sensors. Moreover, the system delivers a fast temperature sensing scheme and it provides coverage to inaccessible areas, thus overcoming the limitations of current mobile platforms which use ground robots. Different experiments were carried out in order to assess the behavior of the sensors as well as to validate the full system. Moreover, the hypothesis that thermopile IR sensors can be used to track temperature signature on-the-fly is validated experimentally with the use of the proposed system over different targets.
Monte Carlo Inference on Two-Sided Matching Models
Authors: Kim Taehoon , Schwartz Jacob , Song Kyungchul , Whang Yoon-Jae
Published: March 2019

This paper considers two-sided matching models with nontransferable utilities, with one side having homogeneous preferences over the other side. When one observes only one or several large matchings, despite the large number of agents involved, asymptotic inference is difficult because the observed matching involves the preferences of all the agents on both sides in a complex way, and creates a complicated form of cross-sectional dependence across observed matches. When we assume that the observed matching is a consequence of a stable matching mechanism with homogeneous preferences on one side, and the preferences are drawn from a parametric distribution conditional on observables, the large observed matching follows a parametric distribution. This paper shows in such a situation how the method of Monte Carlo inference can be a viable option. Being a finite sample inference method, it does not require independence or local dependence among the observations which are often used to obtain asymptotic validity. Results from a Monte Carlo simulation study are presented and discussed.
Put–Call Ratio Volume vs. Open Interest in Predicting Market Return: A Frequency Domain Rolling Causality Analysis
Authors: Jena Sangram Keshari , Tiwari Aviral Kumar , Mitra Amarnath
Published: March 2019

This study examined the efficacy of the Put–Call Ratio (PCR), a widely used information ratio measured in terms of volume and open interest, in predicting market return at different time scale. Volume PCR was found to be an efficient predictor of the market return in a short period of 2.5 days and open interest PCR in a long period of 12 days. Thus, traders and portfolio managers should use the appropriate PCR depending upon the time horizon of their trade and investment. The results are robust even after controlling for the information generated from the futures market.
Tourism Industry and Economic Growth Nexus in Beijing, China
Authors: Songling Yang , Ishtiaq Muhammad , Thanh Bui Thi
Published: March 2019

In the developing economy, tourism is the most visible and steadiest growing facade. Tourism is considered one of the rapidly increasing elements for economic development from the last two decades. Therefore, the proposed study used vector autoregression (VAR) model, error correction model (ECM), and the Granger causality to check the relationship between the tourism industry and economic growth based on the data of the Beijing municipal bureau of statistics from 1994 to 2015. Gross domestic product (GDP) is used as a replacement variable for the economic growth index, while internal tourism revenue is used as a tourism industry indicator. The study supports the tourism-led growth hypothesis proposed in the existing literature in a different survey of tourism and economic development. The results show that there is a strong relationship in the tourism industry and economic growth in the context of Beijing, and at the same time, tourism creates a more significant increase in long run local real economic accomplishments. The results of the VAR model confirm that in the long run, Beijing’s economic growth is affected by domestic tourism, while the ECM model shows unidirectional results in the short term. Similarly, there is a one-way causal relationship between the tourism industry and economic growth in Beijing, China. The empirical results are in strong support of the concept that tourism causes growth.
How Housing Affects Stock Investment—An SEM Analysis
Authors: Li Jiandong , Zhao Jianmei
Published: March 2019

The extant literature regarding the effects of housing on stock investment shows inconsistent findings, either positive or negative effects have been reported. This paper investigates the mechanisms by which housing affects household stock investment through a structure equation model (SEM). Applying the data from the China Household Finance Survey (CHFS), we confirm and quantify the magnitudes of contemporaneous “wealth effects” and “crowd-out effects” of housing on household equity investment. Overall, the combined effect of housing on stock investment is positive in the context of urban China.
Curriculum Design in Construction Engineering Departments for Colleges in Taiwan
Authors: Hsu Wei-Ling , Chen Yi-Sian , Shiau Yan-Chyuan , Liu Hsin-Lung , Chern Tian-Yow
Published: March 2019

Science, technology, engineering, and mathematics (STEM) education has become a new trend in international education curriculum reform. This study analyzed the teaching of seven subjects and performance indicators by collecting data from literature of diversified fields, proficiency training courses, and syllabi of construction management curricula provided by Taiwanese higher education institutions. Research incorporated both qualitative and quantitative methods. Qualitative approaches comprised a literature review and in-depth interview with experts; the quantitative approach was the fuzzy Delphi method, which was used to identify the syllabus constructs of major subjects and rate their performance indicators of secondary subjects. Interpretive structural modelling helped construct a systemic structure and relationships among different types of subjects to analyze curriculum frameworks and systematize teaching models. This study can be referenced to design syllabi for systemic courses in departments of construction engineering and management to educate future construction engineers at higher education institutions.