bluuebunny/crossref_metadata_2025 huggingface dataset:
An energy-free strategy to elevate anti-icing performance of superhydrophobic materials through interfacial airflow manipulation
Authors: Jiang Jiawei , Shen Yizhou , Xu Yangjiangshan , Wang Zhen , Tao Jie , Liu Senyun , Liu Weilan , Chen Haifeng
Published: January 2024

Superhydrophobic surfaces demonstrate excellent anti-icing performance under static conditions. However, they show a marked decrease in icing time under real flight conditions. Here we develop an anti-icing strategy using ubiquitous wind field to improve the anti-icing efficiency of superhydrophobic surfaces during flight. We find that the icing mass on hierarchical superhydrophobic surface with a microstructure angle of 30° is at least 40% lower than that on the conventional superhydrophobic plate, which is attributed to the combined effects of microdroplet flow upwelling induced by interfacial airflow and microdroplet ejection driven by superhydrophobic characteristic. Meanwhile, the disordered arrangement of water molecules induced by the specific 30° angle also raises the energy barriers required for nucleation, resulting in an inhibition of the nucleation process. This strategy of microdroplet movement manipulation induced by interfacial airflow is expected to break through the anti-icing limitation of conventional superhydrophobic materials in service conditions and can further reduce the risk of icing on the aircraft surface.
Global surveillance of antimicrobial resistance in food animals using priority drugs maps
Authors: Zhao Cheng , Wang Yu , Mulchandani Ranya , Van Boeckel Thomas P.
Published: January 2024

Antimicrobial resistance (AMR) in food animals is a growing threat to animal health and potentially to human health. In resource-limited settings, allocating resources to address AMR can be guided with maps. Here, we mapped AMR prevalence in 7 antimicrobials in Escherichia coli and nontyphoidal Salmonella species across low- and middle-income countries (LIMCs), using 1088 point-prevalence surveys in combination with a geospatial model. Hotspots of AMR were predicted in China, India, Brazil, Chile, and part of central Asia and southeastern Africa. The highest resistance prevalence was for tetracycline (59% for E. coli and 54% for nontyphoidal Salmonella, average across LMICs) and lowest for cefotaxime (33% and 19%). We also identified the antimicrobial with the highest probability of resistance exceeding critical levels (50%) in the future (1.7–12.4 years) for each 10 × 10 km pixel on the map. In Africa and South America, 78% locations were associated with penicillins or tetracyclines crossing 50% resistance in the future. In contrast, in Asia, 77% locations were associated with penicillins or sulphonamides. Our maps highlight diverging geographic trends of AMR prevalence across antimicrobial classes, and can be used to target AMR surveillance in AMR hotspots for priority antimicrobial classes.
UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors
Authors: Yang Duomeng , Geng Tingting , Harrison Andrew G. , Cahoon Jason G. , Xing Jian , Jiao Baihai , Wang Mark , Cheng Chao , Hill Robert E. , Wang Huadong , Vella Anthony T. , Cheng Gong , Wang Yanlin , Wang Penghua
Published: January 2024

The Retinoic acid-Inducible Gene I (RIG-I) like receptors (RLRs) are the major viral RNA sensors essential for the initiation of antiviral immune responses. RLRs are subjected to stringent transcriptional and posttranslational regulations, of which ubiquitination is one of the most important. However, the role of ubiquitination in RLR transcription is unknown. Here, we screen 375 definite ubiquitin ligase knockout cell lines and identify Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) as a positive regulator of RLR transcription. UBR5 deficiency reduces antiviral immune responses to RNA viruses, while increases viral replication in primary cells and mice. Ubr5 knockout mice are more susceptible to lethal RNA virus infection than wild type littermates. Mechanistically, UBR5 mediates the Lysine 63-linked ubiquitination of Tripartite Motif Protein 28 (TRIM28), an epigenetic repressor of RLRs. This modification prevents intramolecular SUMOylation of TRIM28, thus disengages the TRIM28-imposed brake on RLR transcription. In sum, UBR5 enables rapid upregulation of RLR expression to boost antiviral immune responses by ubiquitinating and de-SUMOylating TRIM28.
D6PK plasma membrane polarity requires a repeated CXX(X)P motif and PDK1-dependent phosphorylation
Authors: Graf Alina , Bassukas Alkistis Eleftheria Lanassa , Xiao Yao , Barbosa Inês C. R. , Mergner Julia , Grill Peter , Michalke Bernhard , Kuster Bernhard , Schwechheimer Claus
Published: January 2024

D6 PROTEIN KINASE (D6PK) is a polarly localized plasma-membrane-associated kinase from Arabidopsis thaliana that activates polarly distributed PIN-FORMED auxin transporters. D6PK moves rapidly to and from the plasma membrane, independent of its PIN-FORMED targets. The middle D6PK domain, an insertion between kinase subdomains VII and VIII, is required and sufficient for association and polarity of the D6PK plasma membrane. How D6PK polarity is established and maintained remains to be shown. Here we show that cysteines from repeated middle domain CXX(X)P motifs are S-acylated and required for D6PK membrane association. While D6PK S-acylation is not detectably regulated during intracellular transport, phosphorylation of adjacent serine residues, in part in dependence on the upstream 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE, promotes D6PK transport, controls D6PK residence time at the plasma membrane and prevents its lateral diffusion. We thus identify new mechanisms for the regulation of D6PK plasma membrane interaction and polarity.
A fluid biomarker reveals loss of TDP-43 splicing repression in presymptomatic ALS–FTD
Authors: Irwin Katherine E. , Jasin Pei , Braunstein Kerstin E. , Sinha Irika R. , Garret Mark A. , Bowden Kyra D. , Chang Koping , Troncoso Juan C. , Moghekar Abhay , Oh Esther S. , Raitcheva Denitza , Bartlett Dan , Miller Timothy , Berry James D. , Traynor Bryan J. , Ling Jonathan P. , Wong Philip C.
Published: January 2024

Although loss of TAR DNA-binding protein 43 kDa (TDP-43) splicing repression is well documented in postmortem tissues of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), whether this abnormality occurs during early-stage disease remains unresolved. Cryptic exon inclusion reflects loss of function of TDP-43, and thus detection of proteins containing cryptic exon-encoded neoepitopes in cerebrospinal fluid (CSF) or blood could reveal the earliest stages of TDP-43 dysregulation in patients. Here we use a newly characterized monoclonal antibody specific to a TDP-43-dependent cryptic epitope (encoded by the cryptic exon found in HDGFL2) to show that loss of TDP-43 splicing repression occurs in ALS–FTD, including in presymptomatic C9orf72 mutation carriers. Cryptic hepatoma-derived growth factor-like protein 2 (HDGFL2) accumulates in CSF at significantly higher levels in familial ALS–FTD and sporadic ALS compared with controls and is elevated earlier than neurofilament light and phosphorylated neurofilament heavy chain protein levels in familial disease. Cryptic HDGFL2 can also be detected in blood of individuals with ALS–FTD, including in presymptomatic C9orf72 mutation carriers, and accumulates at levels highly correlated with those in CSF. Our findings indicate that loss of TDP-43 cryptic splicing repression occurs early in disease progression, even presymptomatically, and that detection of the HDGFL2 cryptic neoepitope serves as a potential diagnostic biomarker for ALS, which should facilitate patient recruitment and measurement of target engagement in clinical trials.
A multidisciplinary approach to tackling invasive species: barcoding, morphology, and metataxonomy of the leafhopper Arboridia adanae
Authors: Piccinno Riccardo , Tatti Alessia , Avosani Sabina , Galla Giulio , Lazazzara Valentina , Pedrazzoli Federico , Zadra Nicola , Rodeghiero Mirco , Seljak Gabrijel , Özgen İnanç , Hauffe Heidi C. , Verrastro Vincenzo , Stacconi Marco Valerio Rossi , Mazzoni Valerio , Rota-Stabelli Omar
Published: January 2024

The leafhopper genus Arboridia includes several species that feed on Vitis vinifera and cause leaf chlorosis. We report the first alien Arboridia infestation in Italy in 2021 in an Apulian vineyard. To confirm the taxonomic status of the species responsible for crop damage, and reconstruct its demographic history, we barcoded individuals from Apulia together with Arboridia spp. from Crete (Greece), A. adanae from Central Turkey and other specimens of the presumed sister species, A. dalmatina from Dalmatia (Croatia). Molecular phylogenies and barcoding gap analysis identified clades not associated with sampling locations. This result is incongruent with classical specimen assignment and is further supported by morphological analyses, which did not reveal significant differences among the populations. Therefore, we propose A. dalmatina as a junior synonym of A. adanae, which would become the only grapevine-related Arboridia species in the eastern Mediterranean. To further characterise A. adanae evolution, we performed a molecular clock analysis that suggested a radiation during the Pleistocene glaciations. Finally, to assess whether the Apulian individuals carried microorganisms of agricultural relevance, we sequenced their bacterial microbiota using 16S rRNA amplicon sequencing identifying three phytopathogens not generally associated with Arboridia activities as well as Wolbachia in one Apulian haplogroup. We discuss the agricultural implications of this infestation.
Gas adsorption meets deep learning: voxelizing the potential energy surface of metal-organic frameworks
Authors: Sarikas Antonios P. , Gkagkas Konstantinos , Froudakis George E.
Published: January 2024

Intrinsic properties of metal-organic frameworks (MOFs), such as their ultra porosity and high surface area, deem them promising solutions for problems involving gas adsorption. Nevertheless, due to their combinatorial nature, a huge number of structures is feasible which renders cumbersome the selection of the best candidates with traditional techniques. Recently, machine learning approaches have emerged as efficient tools to deal with this challenge, by allowing researchers to rapidly screen large databases of MOFs via predictive models. The performance of the latter is tightly tied to the mathematical representation of a material, thus necessitating the use of informative descriptors. In this work, a generalized framework to predict gaseous adsorption properties is presented, using as one and only descriptor the capstone of chemical information: the potential energy surface (PES). In order to be machine understandable, the PES is voxelized and subsequently a 3D convolutional neural network (CNN) is exploited to process this 3D energy image. As a proof of concept, the proposed pipeline is applied on predicting $${\hbox {CO}_{2}}$$

CO
2

uptake in MOFs. The resulting model outperforms a conventional model built with geometric descriptors and requires two orders of magnitude less training data to reach a given level of performance. Moreover, the transferability of the approach to different host-guest systems is demonstrated, examining $${\hbox {CH}_4}$$

CH
4

uptake in COFs. The generic character of the proposed methodology, inherited from the PES, renders it applicable to fields other than reticular chemistry.
Direct comparison of canine and human immune responses using transcriptomic and functional analyses
Authors: Chow Lyndah , Wheat William , Ramirez Dominique , Impastato Renata , Dow Steven
Published: January 2024

The canine spontaneous cancer model is increasingly utilized to evaluate new combined cancer immunotherapy approaches. While the major leukocyte subsets and phenotypes are closely related in dogs and humans, the functionality of T cells and antigen presenting cells in the two species has not been previously compared in detail. Such information would be important in interpreting immune response data and evaluating the potential toxicities of new cancer immunotherapies in dogs. To address this question, we used in vitro assays to compare the transcriptomic, cytokine, and proliferative responses of activated canine and human T cells, and also compared responses in activated macrophages. Transcriptomic analysis following T cell activation revealed shared expression of 515 significantly upregulated genes and 360 significantly downregulated immune genes. Pathway analysis identified 33 immune pathways shared between canine and human activated T cells, along with 34 immune pathways that were unique to each species. Activated human T cells exhibited a marked Th1 bias, whereas canine T cells were transcriptionally less active overall. Despite similar proliferative responses to activation, canine T cells produced significantly less IFN-γ than human T cells. Moreover, canine macrophages were significantly more responsive to activation by IFN-γ than human macrophages, as reflected by co-stimulatory molecule expression and TNF-α production. Thus, these studies revealed overall broad similarity in responses to immune activation between dogs and humans, but also uncovered important key quantitative and qualitative differences, particularly with respect to T cell responses, that should be considered in designing and evaluating cancer immunotherapy studies in dogs.
Multifunctional role of dietary copper to regulate stress-responsive gene for mitigation of multiple stresses in Pangasianodon hypophthalmus
Authors: Kumar Neeraj , Thorat Supriya Tukaram , Chavhan Samiksha R.
Published: January 2024

It is an urgent needs to address climate change and pollution in aquatic systems using suitable mitigation measures to avoid the aquatic animals' extinction. The vulnerability and extinction of the aquatic animals in the current scenario must be addressed to enhance safe fish food production. Taking into consideration of such issues in fisheries and aquaculture, an experiment was designed to mitigate high temperature (T) and low pH stress, as well as arsenic (As) pollution in fish using copper (Cu) containing diets. In the present investigation, the Cu-containing diets graded with 0, 4, 8, and 12 mg kg-1 were prepared and fed to Pangasianodon hypophthalmus reared under As, low pH, and high-temperature stress. The gene expression was highly affected in terms of the primary, secondary, and tertiary stress response, whereas supplementation of Cu-containing diet mitigates the stress response. Oxidative stress genes such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were significantly upregulated by stressors (As, As + T, and As + pH + T). Whereas, heat shock protein (HSP 70), inducible nitric oxide synthase (iNOS), metallothionine (MT), caspase 3a (Cas 3a), and cytochrome P450 (CYP 450) were highly upregulated by stressors, while dietary Cu at 8 mg kg-1 diet significantly downregulated these gene expressions. Indeed, the immunity-related genes viz. TNFα, Ig, TLR, and immune-related attributes viz. albumin, globulin, total protein, A:G ratio, blood glucose, NBT, and myeloperoxidase (MPO) were also improved with Cu-containing diets. Cu containing diets substantially improved neurotransmitter enzyme (AChE) and vitamin C (Vit C). DNA damage was also reduced with supplementation of Cu at 8 mg kg-1 diet. The growth index viz. final body weight gain (%), specific growth rate, protein efficiency ratio, food conversion ratio, relative feed intake, and daily growth index were noticeably enhanced by Cu diets (4 and 8 mg kg-1 diet). The growth-related genes expressions viz. growth hormone (GH), growth hormone regulator 1 (Ghr1), growth hormone regulator β (Ghrβ,) myostatin (MYST), and somatostatin (SMT) supported the growth enhancement with Cu at 8 mg kg-1 diet. The bioaccumulation of As was reduced with Cu-containing diets. The fish were infected with Aeromonas hydrophila at the end of the 105 days experimental trial. Cu at 8 mg kg-1 diet improved immunity, reduced the cumulative mortality, and enhanced the relative percentage survival of the fish. The results revealed that the innovative Cu diets could reduce the extinction of the fish against climate change and pollution era and produce the safest production that is safe to humans for consumption.
Potential impact on using aspirin as the primary prevention of adverse pregnancy outcomes in twins conceived using ART
Authors: Huang Dongni , Xie Yao , Duan Pingmei , Wang Jiaxin , Xu Jiacheng , Qi Hongbo , Luo Xin
Published: January 2024

With the development of assisted reproductive technology, the number of twin pregnancies is increasing year by year. Given the increased risk of pregnancy complications associated with twin pregnancies, and the fact that these babies are rare and difficult to obtain through assisted reproductive technology, clinicians urgently require finding effective and safe drugs to improve pregnancy outcomes. Low-dose aspirin can not only promote placental blood supply, but also effectively anti-inflammatory. Whether Low-dose aspirin can effectively reduce the risk of pregnancy complications in this special group needs to be clarified. We therefore retrospectively analyzed 665 twin pregnancies from assisted reproduction technology, grouped according to aspirin use, and followed pregnancy outcomes to assess bleeding risk. Low-dose aspirin was found to be effective in preventing preeclampsia without a significant risk of bleeding. However, aspirin does not prevent specific complication in twin pregnancies and seems to have a better preventive effect only when the mother is under 30, which should alarm clinicians should not blindly using aspirin in this particular group.
Prosaposin variants in sporadic, familial, and early-onset Parkinson's disease: a Taiwanese case–control study and meta-analysis
Authors: Kuo Ming-Che , Chu Yung-Tsai , Su Yu-An , Chen Meng-Ling , Wu Ruey-Meei
Published: January 2024

Polymorphisms in the PSAP gene, which encodes prosaposin and is involved in the lysosomal function, yielded conflicting results regarding the association with Parkinson’s disease (PD). Therefore, this study aims to investigate the role of PSAP in familial PD (FPD), early onset PD (EOPD) with age at onset before 50 years old, and sporadic PD (SPD) among Taiwanese population, and summarize relevant studies via meta-analysis. By sequencing exon 1 to 14 in 183 FPD and 219 EOPD, two novel exonic variants were found in EOPD, including p.A146E (c.437C > A) on exon 5 and p.Y248C (c.743A > G) on exon 7. Furthermore, four previously reported intronic variants (rs142614739/rs74733861), rs749823, rs4747203 and rs885828) in intron 11 and 12 were analyzed in 485 SPD and 712 in-hospital controls, in addition to the aforementioned FPD and EOPD groups. The adjusted odd ratios (ORs) by age and sex, only rs142614739 was significantly associated with higher risk of EOPD (OR = 1.85, 95% CI = 1.33–2.58). The risk effect was further confirmed by the meta-analysis of the association between rs142614739 and the risk of PD in both common effect (OR = 1.29, 95% CI = 1.11–1.50) and random effect (OR = 1.29, 95% CI = 1.11–1.50). Our findings suggest that the PSAP rs142614739 variant is associated with the risk of EOPD. Further functional studies are warranted to elucidate the biochemical mechanisms.
A systematic review and meta-analysis of how social memory is studied
Authors: Cum Meghan , Santiago Pérez Jocelyn A. , Wangia Erika , Lopez Naeliz , Wright Elizabeth S. , Iwata Ryo L. , Li Albert , Chambers Amelia R. , Padilla-Coreano Nancy
Published: January 2024

Social recognition is crucial for survival in social species, and necessary for group living, selective reproduction, pair bonding, and dominance hierarchies. Mice and rats are the most commonly used animal models in social memory research, however current paradigms do not account for the complex social dynamics they exhibit in the wild. To assess the range of social memories being studied, we conducted a systematic analysis of neuroscience articles testing the social memory of mice and rats published within the past two decades and analyzed their methods. Our results show that despite these rodent’s rich social memory capabilities, the majority of social recognition papers explore short-term memories and short-term familiarity levels with minimal exposure between subject and familiar stimuli—a narrow type of social memory. We have identified several key areas currently understudied or underrepresented: kin relationships, mates, social ranks, sex variabilities, and the effects of aging. Additionally, reporting on social stimulus variables such as housing history, strain, and age, is limited, which may impede reproducibility. Overall, our data highlight large gaps in the diversity of social memories studied and the effects social variables have on social memory mechanisms.
Demonstration of Japanese radiographic examination codes in establishing typical values for a wide variety of general radiography examinations
Authors: Yagahara Ayako , Ando Daisuke , Oda Makoto
Published: January 2024

The purpose of this study was to demonstrate Japanese radiographic examination codes JJ1017 in establishing typical values for a wide variety of general radiography. About 200,000 sets of examination data were collected, including exposure conditions, JJ1017 code applied, examination room numbers and patient information. Typical values for adults, children, and infants were calculated from the collected data, and the following items were examined: comparing typical values of general radiography in Japan DRLs 2015 and typical values in a facility; comparison of typical values between X-ray equipment for examinations of DRLs 2015; comparison of typical values for different procedures at the same anatomical site; identification of examination items associated with high radiation doses. The total numbers of JJ1017 codes applicable to the examinations were 45,372 for adults, 542 for children, and 2339 for infants. To calculate the typical values and compare these with the DRLs, we used a combination of JJ1017 anatomical codes, posture codes, and direction of radiation codes. The combination of these codes allowed the calculation of a typical value and comparison with DRLs 2015. Comparison between devices reveals differences in radiation doses and provides an opportunity to review the characteristics of the devices and their operation to suggest dose reductions. By calculating typical values for examination items for which the DRLs were not available, we were able to identify examination items with high doses in a facility and suggest items that should be audited in the facility.
Enhanced prediction of bolt support drilling pressure using optimized Gaussian process regression
Authors: Liu Jie
Published: January 2024

This study introduces a novel method for predicting drilling pressure in bolt support systems by optimizing Gaussian process time series regression (GPR) using hybrid optimization algorithms. The research initially identified significant variations in prediction outcomes based on different kernel functions and historical points combinations in the GPR algorithm. To address this, we explored 160 distinct schemes combining 10 kernel functions and 16 historical points for numerical analysis. Applying three hybrid optimization algorithms—Genetic Algorithm-GPR (GA-GPR), Particle Swarm Optimization-GPR (PSO-GPR), and Ant Colony Algorithm-GPR (ACA-GPR)—we iteratively optimized these key parameters. The PSO-GPR algorithm emerged as the most effective, achieving an 80% prediction accuracy with a deviation range of 1–2 MPa, acceptable in practical drilling operations. This optimization led to the RQ kernel function with 18 historical points as the optimal combination, yielding an RMSE value of 0.0047246, in contrast to the least effective combination (E kernel function with 6 historical points) producing an RMSE of 0.035704. The final outcome of this study is a robust and efficient prediction system for underground bolt support drilling pressure, verified through practical application. This approach significantly enhances the accuracy and efficiency of support systems in geotechnical engineering, demonstrating the practical applicability of the PSO-GPR model in real-world scenarios.
The chronic wound milieu changes essential oils' antibiofilm activity—an in vitro and larval model study
Authors: Brożyna Malwina , Dudek Bartłomiej , Kozłowska Weronika , Malec Katarzyna , Paleczny Justyna , Detyna Jerzy , Fabianowska-Majewska Krystyna , Junka Adam
Published: January 2024

Essential Oils (EOs) are currently being researched as potential antibiofilm agents to combat infections related to chronic wound biofilms. As documented in the literature, EOs’ in vitro antibacterial properties are often assessed using standard microbiological media and conditions that do not accurately reflect the actual environment of a chronic wound. To address this issue, In vitro Wound Milieu (IVWM) medium, which closely resembles the environment of a chronic wound, was applied for culturing S. aureus biofilms (n = 12) in this research. Biofilms cultivated in the standard Tryptic Soy Broth (TSB) medium served as a control for the experiment. Key biofilm features were analyzed and compared. Subsequently, staphylococci were exposed to the activity of thyme or rosemary EOs (T-EO and R-EO, respectively). As proof of concept, the cytotoxicity of T-EO and its antimicrobial in vivo activity were assessed using a G. mellonella larvae model. Key features of biofilm-forming cells were lower in the IVWM than in the TSB medium: biomass (up to 8 times), metabolic activity (up to 9 times), cell number (up to 100 times), and the live/dead cells ratio. Conversely, biofilm thickness was higher (up to 25%) in IVWM. These differences translated into varied responses of the biofilms to EOs exposure. The application of T-EO led to a greater reduction (up to 2 times) in 67% of biofilm-forming strains in IVWM compared to the TSB medium. Conversely, exposure to R-EO resulted in a higher reduction (up to 2.6 times) of 83% of biofilm-forming strains in TSB than in IVWM. The application of T-EO was not only non-toxic to G. mellonella larvae but also increased the survival of larvae infected with staphylococci (from 48 to 85%). Our findings suggest that EOs not only show promise as agents for treating biofilm-related wound infections but also that providing conditions reflecting the specific niche of the human body is of paramount importance in influencing the results obtained. However, before clinical application, challenges related to the methods of assessing their activity, microbial intra-species variability, and different levels of activity of various EOs should be analyzed and standardized.
Bursts of communication increase opinion diversity in the temporal Deffuant model
Authors: Zarei Fatemeh , Gandica Yerali , Rocha Luis E. C.
Published: January 2024

Human interactions create social networks forming the backbone of societies. Individuals adjust their opinions by exchanging information through social interactions. Two recurrent questions are whether social structures promote opinion polarisation or consensus and whether polarisation can be avoided, particularly on social media. In this paper, we hypothesise that not only network structure but also the timings of social interactions regulate the emergence of opinion clusters. We devise a temporal version of the Deffuant opinion model where pairwise social interactions follow temporal patterns. Individuals may self-organise into a multi-partisan society due to network clustering promoting the reinforcement of local opinions. Burstiness has a similar effect and is alone sufficient to refrain the population from consensus and polarisation by also promoting the reinforcement of local opinions. The diversity of opinions in socially clustered networks thus increases with burstiness, particularly, and counter-intuitively, when individuals have low tolerance and prefer to adjust to similar peers. The emergent opinion landscape is well-balanced regarding groups’ size, with relatively short differences between groups, and a small fraction of extremists. We argue that polarisation is more likely to emerge in social media than offline social networks because of the relatively low social clustering observed online, despite the observed online burstiness being sufficient to promote more diversity than would be expected offline. Increasing the variance of burst activation times, e.g. by being less active on social media, could be a venue to reduce polarisation. Furthermore, strengthening online social networks by increasing social redundancy, i.e. triangles, may also promote diversity.
The 6 month interim analysis of a randomized controlled trial assessing the quality of life in patients with breast cancer related lymphedema undergoing lymphaticovenous anastomosis vs. conservative therapy
Authors: Jonis Y. M. J. , Wolfs J. A. G. N. , Hummelink S. , Tielemans H. J. P. , Keuter X. H. A. , van Kuijk S. , Ulrich D. J. O. , van der Hulst R. R. W. J. , Qiu S. S.
Published: January 2024

Breast cancer related lymphedema (BCRL) is a chronic condition with a detrimental impact on psychosocial and physical well-being. Lymphaticovenous anastomosis has shown promising results in alleviating physical symptoms and increasing quality of life in patients with BCRL. The aim of the study is to evaluate the effect on health related quality of life (HrQol) after LVA surgery versus conservative treatment in patients with BCRL. The study is a prospective, multicenter randomized controlled trial. Adult women with unilateral BCRL, with early stage lymphedema and viable lymphatic vessels were included. The primary outcome measure was HrQol measured by the lymphedema functioning disability and health (Lymph-ICF) questionnaire. The secondary outcomes were volume difference measured by the water displacement method; the Upper Extremity Lymphedema (UEL) index; and daily use of the compression garments after 3 and 6 months. For this interim analysis 46 patients per group were included. There was a significant improvement in the domains in physical and mental function in the Lymph-ICF questionnaire in the LVA group after 6 months, (− 16.46 ± 18.5, p < 0.05, − 10.12 ± 29.5, p < 0.05 respectively). However, there was no statistical difference in the total score of the Lymph-ICF after 6 months in both groups (LVA-group; − 8.57 ± 22.6, p > 0.05, CDT-group; − 2.65 ± 18.2, p < 0.05). Furthermore, there was no significant volume reduction in both groups (LVA-group: 20.04 ± 196.40, p = 0.497, CDT: 33.98 ± 189.87, p = 0.236). In the LVA group, 41% partially of completely stopped wearing the compression garments after six months whereas in the CDT group 0% discontinued to use of compression garments. LVA resulted in improvement of the domains physical and mental function of the Lymph-ICF. Limb volume did not significantly improve after 6 months. However, around 42% could completely or partially stopped with the use of compression garments in the LVA group. The current results are promising, however longer follow up is required to assess long term effect of LVA for secondary lymphedema.

Clinical Trial Registration: NCT02790021 registered on 03/06/2016
Sex-specific impact of mild obesity on the prognosis of ST-segment elevation myocardial infarction
Authors: Zhang Lingling , Liu Zhican , Zhu Yunlong , Zeng Jianping , Huang Haobo , Yang Wenbin , Peng Ke , Wu Mingxin
Published: January 2024

This study aimed to clarify the existence of the mild obesity paradox in patients with ST-segment elevation myocardial infarction (STEMI) and assess the impact of mild obesity on the prognosis of STEMI. A retrospective cohort study was conducted on STEMI patients who underwent percutaneous coronary intervention at Xiangtan Central Hospital from January 1, 2020 to July 31, 2022. After excluding individuals with a body mass index (BMI) of no less than 35 kg/m2, subjects were divided into the mildly obese group (BMI, 30–35 kg/m2) and non-obese group (BMI < 30 kg/m2). The cardiovascular events and death were deemed the composite endpoints and were employed as the outcome event. The study recruited 664 patients with STEMI, including 515 males and 149 females. The mildly obese group of male patients exhibited a lower incidence of composite endpoints than the non-obese group (22.4% vs. 41.3%, P < 0.001). For female patients, no significant difference was observed in the incidence of composite endpoints between the two groups (43.6% vs. 43.8%, P = 0.987). After adjusting for confounding factors, the multivariable Cox regression analysis revealed mild obesity as an independent protective factor for male patients [hazard ratio (HR) 0.47; 95% confidence interval (CI) 0.32–0.69; P < 0.001]. Nevertheless, mild obesity was not associated with the prognosis of female patients (HR 0.96; 95% CI 0.47–1.94; P = 0.9). In male STEMI patients, mild obesity presented a paradoxical effect in improving the prognosis and functioned as an independent protective factor for the prognosis of STEMI. However, no association between mild obesity and prognosis was found in female patients, possibly due to distinct physiological and metabolic characteristics between male and female patients, which deserved further investigation and validation.
Comprehensive studies for evaluating promising properties of Cu/graphene/fly ash nanocomposites
Authors: El-Zaidia M. M. , Zaki Mai Z. , Abomostafa H. M. , Taha Mohammed A.
Published: January 2024

Copper (Cu)'s electrical conductivity makes it attractive for industrial usage. Due to its inferior mechanical characteristics, thermal expansion, and wear resistance, its applications are limited. This manuscript solves these issues while retaining its major feature, excellent electrical conductivity. In this regard, different quantities of graphene (Gr) and fly ash (FA) nanoparticles were combined with Cu in a planetary ball mill at 440 rpm for 20 h using powder metallurgy (PM). The microstructure of the generated powders was characterized using X-ray diffraction technique and transmission electron microscopy. The powders underwent compression and were then subjected to firing at three distinct temperature levels, reaching a maximum of 850 °C. In addition, an analysis was conducted on the microstructure, mechanical properties, wear resistance, thermal expansion behaviour, and electrical conductivity of the sintered samples. Based on the findings, the inclusion of a hybrid of Gr and FA ceramics effectively led to a reduction in particle sizes. The bulk density slightly decreases with the addition of hybrid ceramic while increasing with the rise in sintering temperature. The hybrid composited Cu/0.8 vol.% Gr/8 vol.% FA recorded an increase in the microhardness, ultimate stress, and Young’s modulus of 25, 20, and 50%, respectively, relative to the Cu matrix. Furthermore, the wear rate and coefficient of thermal expansion for the same sample decreased by 67 and 30%, respectively. Finally, increasing the sintering temperature showed a clear improvement in the mechanical, electrical, and corrosion properties. Based on the results obtained, it can be concluded that the prepared hybrid nanocomposites can be used in power generation, power transmission, electronic circuits, and other applications.
Genomic analysis of Nigerian indigenous chickens reveals their genetic diversity and adaptation to heat-stress
Authors: Rachman Mifta P. , Bamidele Oladeji , Dessie Tadelle , Smith Jacqueline , Hanotte Olivier , Gheyas Almas A.
Published: January 2024

Indigenous poultry breeds from Africa can survive in harsh tropical environments (such as long arid seasons, excessive rain and humidity, and extreme heat) and are resilient to disease challenges, but they are not productive compared to their commercial counterparts. Their adaptive characteristics are in response to natural selection or to artificial selection for production traits that have left selection signatures in the genome. Identifying these signatures of positive selection can provide insight into the genetic bases of tropical adaptations observed in indigenous poultry and thereby help to develop robust and high-performing breeds for extreme tropical climates. Here, we present the first large-scale whole-genome sequencing analysis of Nigerian indigenous chickens from different agro-climatic conditions, investigating their genetic diversity and adaptation to tropical hot climates (extreme arid and extreme humid conditions). The study shows a large extant genetic diversity but low level of population differentiation. Using different selection signature analyses, several candidate genes for adaptation were detected, especially in relation to thermotolerance and immune response (e.g., cytochrome P450 2B4-like, TSHR, HSF1, CDC37, SFTPB, HIF3A, SLC44A2, and ILF3 genes). These results have important implications for conserving valuable genetic resources and breeding improvement of chickens for thermotolerance.
miRNA profiling of chicken follicles during follicular development
Authors: Xu Zhongxian , Liu Qian , Ning Chunyou , Yang Maosen , Zhu Qing , Li Diyan , Wang Tao , Li Feng
Published: January 2024

MicroRNAs (miRNAs) play a crucial role as transcription regulators in various aspects of follicular development, including steroidogenesis, ovulation, apoptosis, and gene regulation in poultry. However, there is a paucity of studies examining the specific impact of miRNAs on ovarian granulosa cells (GCs) across multiple grades in laying hens. Consequently, this study aims to investigate the roles of miRNAs in chicken GCs. By constructing miRNA expression profiles of GCs at 10 different time points, encompassing 4 pre-hierarchical, 5 preovulatory, and 1 postovulatory follicles stage, we identified highly expressed miRNAs involved in GC differentiation (miR-148a-3p, miR-143-3p), apoptosis (let7 family, miR-363-3p, miR-30c-5p, etc.), and autophagy (miR-128-3p, miR-21-5p). Furthermore, we discovered 48 developmentally dynamic miRNAs (DDMs) that target 295 dynamic differentially expressed genes (DDGs) associated with follicular development and selection (such as oocyte meiosis, progesterone-mediated oocyte maturation, Wnt signaling pathway, TGF-β signaling pathway) as well as follicular regression (including autophagy and cellular senescence). These findings contribute to a more comprehensive understanding of the intricate mechanisms underlying follicle recruitment, selection, and degeneration, aiming to enhance poultry’s reproductive capacity.
The investigation of diesel soot emission using instrument combination of multi-wavelength photoacoustic spectroscopy and scanning mobility particle sizer
Authors: Ajtai Tibor , Utry Noémi , Pintér Máté , Rahman Abdul , Kurilla Boldizsár , Sárossy Gábor , Deák László , Baladincz Jenő , Raffai Péter , Szabó Gábor , Bozóki Zoltán
Published: January 2024

The parallel measurements of wavelength dependent optical absorption, particle number size distribution have made by a multi wavelength photoacoustic spectrometer (4λ-PAS) and scanning mobility particle sizer (SMPS) respectively at different modes of a diesel engine using two different types of fuel. The thermal evolution of the emission was also investigated using posterior temperature treatment of emission. The bimodal size distribution of emitted particles at a set reference temperature has been observed regardless of the applied fuel at idle. However, the emitted particulate assembly had lognormal size distribution falls into the accumulation mode at all other defined engine modes and both fuel types. The total number- and volume concentration (TNC and TVC) showed retrograde tendency with the increasing torque and rpm independently of the applied fuel types. The TNC values decreased up to 50% for both fuels with engine operation changes from idle engine mode(em#1) to low engine mode(em#2). With further increase in torque and rpm of engine, the change in TNC is negligible. On the other hand, the TVC remains more or less the same for idle to low engine mode transition and increased more than 60% for high mode (em#3) transition. The Optical Absorption Coefficient (OAC) values measured at the operational wavelengths of the 4λ-PAS instrument decreased at all wavelengths with increasing rpm and torque. The wavelength dependency quantified by Aerosol Ängström Exponent (AAE) was applied here for qualitative analysis of the carbonaceous emission and showed decreased values towards the higher engine speed and torque output of the engine. The proposed technique can be used as real-time, precise and accurate measurement of light absorption by DPM aerosols, which opens up novel possibilities for the volatility and thermal evolution investigation of diesel emissions.
Cortistatin prevents glucocorticoid-associated osteonecrosis of the femoral head via the GHSR1a/Akt pathway
Authors: Gao Yuan , You Yunhao , Zhang Pengfei , Yu Yang , Xu Zhaoning , Wei Hui , Liu Zhicheng , Yu Ruixuan , Jin Gaoxin , Wang Hao , Zhang Shuai , Li Yuhua , Li Weiwei
Published: January 2024

Long-term use of glucocorticoids (GCs) is known to be a predominant cause of osteonecrosis of the femoral head (ONFH). Moreover, GCs can mediate apoptosis of various cell types by exaggerating oxidative stress. We have previously found that Cortistatin (CST) antagonizes oxidative stress and improves cell apoptosis in several conditions. In this study, we detected that the CST expression levels were diminished in patients with ONFH compared with femoral neck fracture (FNF). In addition, a GC-induced rat ONFH model was established, which impaired bone quality in the femoral head. Then, administration of CST attenuated these ONFH phenotypes. Furthermore, osteoblast and endothelial cells were cultured and stimulated with dexamethasone (Dex) in the presence or absence of recombinant CST. As a result, Dex induced impaired anabolic metabolism of osteoblasts and suppressed tube formation in endothelial cells, while additional treatment with CST reversed this damage to the cells. Moreover, blocking GHSR1a, a well-accepted receptor of CST, or blocking the AKT signaling pathway largely abolished the protective function of CST in Dex-induced disorder of the cells. Taken together, we indicate that CST has the capability to prevent GC-induced apoptosis and metabolic disorder of osteoblasts in the pathogenesis of ONFH via the GHSR1a/AKT signaling pathway.
Genetic architecture and biology of youth-onset type 2 diabetes
Authors: Kwak Soo Heon , Srinivasan Shylaja , Chen Ling , Todd Jennifer , Mercader Josep M. , Jensen Elizabeth T. , Divers Jasmin , Mottl Amy K. , Pihoker Catherine , Gandica Rachelle G. , Laffel Lori M. , Isganaitis Elvira , Haymond Morey W. , Levitsky Lynne L. , Pollin Toni I. , Florez Jose C. , Flannick Jason ,   Progress in Diabetes Genetics in Youth (ProDiGY) consortium
Published: January 2024

The prevalence of youth-onset type 2 diabetes (T2D) and childhood obesity has been rising steadily1, producing a growing public health concern1 that disproportionately affects minority groups2. The genetic basis of youth-onset T2D and its relationship to other forms of diabetes are unclear3. Here we report a detailed genetic characterization of youth-onset T2D by analysing exome sequences and common variant associations for 3,005 individuals with youth-onset T2D and 9,777 adult control participants matched for ancestry, including both males and females. We identify monogenic diabetes variants in 2.4% of individuals and three exome-wide significant (P < 2.6 × 10−6) gene-level associations (HNF1A, MC4R, ATXN2L). Furthermore, we report rare variant association enrichments within 25 gene sets related to obesity, monogenic diabetes and β-cell function. Many youth-onset T2D associations are shared with adult-onset T2D, but genetic risk factors of all frequencies—and rare variants in particular—are enriched within youth-onset T2D cases (5.0-fold increase in the rare variant and 3.4-fold increase in common variant genetic liability relative to adult-onset cases). The clinical presentation of participants with youth-onset T2D is influenced in part by the frequency of genetic risk factors within each individual. These findings portray youth-onset T2D as a heterogeneous disease situated on a spectrum between monogenic diabetes and adult-onset T2D.
Global spatially explicit yield gap time trends reveal regions at risk of future crop yield stagnation
Authors: Gerber James S. , Ray Deepak K. , Makowski David , Butler Ethan E. , Mueller Nathaniel D. , West Paul C. , Johnson Justin A. , Polasky Stephen , Samberg Leah H. , Siebert Stefan , Sloat Lindsey
Published: January 2024

Yield gaps, here defined as the difference between actual and attainable yields, provide a framework for assessing opportunities to increase agricultural productivity. Previous global assessments, centred on a single year, were unable to identify temporal variation. Here we provide a spatially and temporally comprehensive analysis of yield gaps for ten major crops from 1975 to 2010. Yield gaps have widened steadily over most areas for the eight annual crops and remained static for sugar cane and oil palm. We developed a three-category typology to differentiate regions of ‘steady growth’ in actual and attainable yields, ‘stalled floor’ where yield is stagnated and ‘ceiling pressure’ where yield gaps are closing. Over 60% of maize area is experiencing ‘steady growth’, in contrast to ∼12% for rice. Rice and wheat have 84% and 56% of area, respectively, experiencing ‘ceiling pressure’. We show that ‘ceiling pressure’ correlates with subsequent yield stagnation, signalling risks for multiple countries currently realizing gains from yield growth.
D–A–D organic fluorescent probes for NIR-II fluorescence imaging and efficient photothermal therapy of breast cancer
Authors: Gao Jie , Yuan Lin , Min Yu , Yu Bing , Cong Hailin , Shen Youqing
Published: January 2024

Schematic illustrations of the preparation of TCT-NPs and their application in NIR-II fluorescence imaging-guided efficient photothermal therapy for breast cancer.
Temperature dependent energy transfer to Eu3+ emission centres in K5Eu(MoO4)4 crystals
Authors: Spassky Dmitry , Vasil'ev Andrey , Jamal Muhammad Usama , Morozov Vladimir A. , Lazoryak Bogdan I. , Redkin Boris S. , Chernenko Kirill , Nagirnyi Vitali
Published: January 2024

Luminescence properties of a palmierite-type K5Eu(MoO4)4 crystal were studied in a wide temperature range of 7–650 K.
Potential-induced degradation: a challenge in the commercialization of perovskite solar cells
Authors: Raza Hasan , Imran Tahir , Gao You , Azeem Muhammad , Younis Muhammad , Wang Jianan , Liu Sanwan , Yang Zhichun , Liu Zonghao , Chen Wei
Published: January 2024

Potential-induced degradation, a major factor in solar cell stability, is a reliability threat that can damage them within a shorter timeframe. As a promising and emerging PV technology, perovskite solar cells must overcome PID to be commercialized.
Influence of contouring the lithium metal/solid electrolyte interface on the critical current for dendrites
Authors: Zhang Shengming , Hu Bingkun , Geng Zeyang , Gao Xiangwen , Spencer-Jolly Dominic , Melvin Dominic L.R. , Ning Ziyang , Li Guanchen , Jenkins Max , Wang Longlong , Gao Hui , Pu Shengda D. , Marrow T. James , Monroe Charles W. , Bruce Peter G.
Published: January 2024

Efforts to avoid dendrites by increasing the interfacial surface area to lower local current densities are limited by significant local pressure accumulation associated with the topography of any surface contouring.
Efficient removal of sulfonamides in complex aqueous environments by an N, P-co-doped graphitic biochar: the crucial role of P2O5
Authors: Tang Wei , Alessi Daniel S. , Wang Tongshuai , Wu Jingqi , Li Shijia , Konhauser Kurt O. , Li Zhixiong , Chen Jiawei
Published: January 2024

It is still unclear what role P species play in sulfonamide removal in AOPs. In this study, we provide a cost-effective solution for the environmental remediation of sulfonamides and establish the role of P2O5 in AOPs.