bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of TGF-β and p38 MAPK in TSG-6 Expression in Adipose Tissue-Derived Stem Cells In Vitro and In Vivo
Authors: Kwon Hye Youn , Yoon Yongdae , Hong Ju-Eun , Rhee Ki-Jong , Sohn Joon Hyung , Jung Pil Young , Kim Moon Young , Baik Soon Koo , Ryu Hoon , Eom Young Woo
Published: December 2023

Mesenchymal stem cells (MSCs) regulate immune cell activity by expressing tumor necrosis factor-α (TNF-α)-stimulated gene 6 (TSG-6) in inflammatory environments; however, whether anti-inflammatory responses affect TSG-6 expression in MSCs is not well understood. Therefore, we investigated whether transforming growth factor-β (TGF-β) regulates TSG-6 expression in adipose tissue-derived stem cells (ASCs) and whether effective immunosuppression can be achieved using ASCs and TGF-β signaling inhibitor A83-01. TGF-β significantly decreased TSG-6 expression in ASCs, but A83-01 and the p38 inhibitor SB202190 significantly increased it. However, in septic C57BL/6 mice, A83-01 further reduced the survival rate of the lipopolysaccharide (LPS)-treated group and ASC transplantation did not improve the severity induced by LPS. ASC transplantation alleviated the severity of sepsis induced by LPS+A83-01. In co-culture of macrophages and ASCs, A83-01 decreased TSG-6 expression whereas A83-01 and SB202190 reduced Cox-2 and IDO-2 expression in ASCs. These results suggest that TSG-6 expression in ASCs can be regulated by high concentrations of pro-inflammatory cytokines in vitro and in vivo, and that A83-01 and SB202190 can reduce the expression of immunomodulators in ASCs. Therefore, our data suggest that co-treatment of ASCs with TGF-β or p38 inhibitors is not adequate to modulate inflammation.
The bZIP Transcription Factors in Current Jasmine Genomes: Identification, Characterization, Evolution and Expressions
Authors: Zhao Kai , Luo Xianmei , Shen Mingli , Lei Wen , Lin Siqing , Lin Yingxuan , Sun Hongyan , Ahmad Sagheer , Wang Guohong , Liu Zhong-Jian
Published: December 2023

Jasmine, a recently domesticated shrub, is renowned for its use as a key ingredient in floral tea and its captivating fragrance, showcasing significant ornamental and economic value. When cultivated to subtropical zone, a significant abiotic stress adaptability occurs among different jasmine varieties, leading to huge flower production changes and plantlet survival. The bZIP transcription factors (TFs) are reported to play indispensable roles in abiotic stress tolerance. Here, we performed a genome-level comparison of bZIPs using three-type jasmine genomes. Based on their physicochemical properties, conserved motif analysis and phylogenetic analysis, about 63 bZIP genes were identified and clustered in jasmine genomes, noting a difference of one member compared to the other two types of jasmines. The HTbZIP genes were categorized into 12 subfamilies compared with A. thaliana. In cis-acting element analysis, all genes contained light-responsive elements. The abscisic acid response element (ABRE) was the most abundant in HTbZIP62 promoter, followed by HTbZIP33. Tissue-specific genes of the bZIPs may play a crucial role in regulating the development of jasmine organs and tissues, with HTbZIP36 showing the most significant expressions in roots. Combined with complicated protein interactions, HTbZIP62 and HTbZIP33 might play a crucial role in the ABA signaling pathway and stress tolerance. Combined with RT-qPCR analysis, SJbZIP37/57/62 were more sensitive to ABA response genes compared with other bZIPs in DJ amd HT genomes. Our findings provide a useful resource for further research on the regulation of key genes to improve abiotic stress tolerance in jasmine.
Salt and Temperature Effects on Xanthan Gum Polysaccharide in Aqueous Solutions
Authors: Nsengiyumva Emmanuel M. , Heitz Mark P. , Alexandridis Paschalis
Published: December 2023

Xanthan gum (XG) is a carbohydrate polymer with anionic properties that is widely used as a rheology modifier in various applications, including foods and petroleum extraction. The aim was to investigate the effect of Na+, K+, and Ca2+ on the physicochemical properties of XG in an aqueous solution as a function of temperature. Huggins, Kraemer, and Rao models were applied to determine intrinsic viscosity, [η], by fitting the relative viscosity (ηrel) or specific viscosity (ηsp) of XG/water and XG/salt/water solutions. With increasing temperature in water, Rao 1 gave [η] the closest to the Huggins and Kraemer values. In water, [η] was more sensitive to temperature increase (~30% increase in [η], 20–50 °C) compared to salt solutions (~15–25% increase). At a constant temperature, salt counterions screened the XG side-chain-charged groups and decreased [η] by up to 60% over 0.05–100 mM salt. Overall, Ca2+ was much more effective than the monovalent cations in screening charge. As the salt valency and concentration increased, the XG coil radius decreased, making evident the effect of shielding the intramolecular and intermolecular XG anionic charge. The reduction in repulsive forces caused XG structural contraction. Further, higher temperatures led to chain expansion that facilitated increased intermolecular interactions, which worked against the salt effect.
Downregulation of γ-Catenin by miR-195-5p Inhibits Colon Cancer Progression, Regulating Desmosome Function
Authors: Piccinno Emanuele , Scalavino Viviana , Labarile Nicoletta , Bianco Giusy , Savino Maria Teresa , Armentano Raffaele , Giannelli Gianluigi , Serino Grazia
Published: December 2023

Desmosomes are essential structures for ensuring tissue functions, and their deregulation is involved in the development of colorectal cancer (CRC). JUP (γ-catenin) is a desmosome adhesion component that also acts as a signaling hub, suggesting its potential involvement in CRC progression. In this context, we recently demonstrated that miR-195-5p regulated JUP and desmosome cadherins expression. In addition, miR-195-5p gain of function indirectly modulated the expression of key effectors of the Wnt pathway involved in JUP-dependent signaling. Here, our purpose was to demonstrate the aberrant expression of miR-195-5p and JUP in CRC patients and to functionally characterize the role of miR-195-5p in the regulation of desmosome function. First, we showed that miR-195-5p was downregulated in CRC tumors compared to adjacent normal tissue. Then, we demonstrated that JUP expression was significantly increased in CRC tissues compared to adjacent normal tissues. The effects of miR-195-5p on CRC progression were assessed using in vitro transient transfection experiments and in vivo miRNA administration. Increased miR-195-5p in colonic epithelial cells strongly inhibits cell proliferation, viability, and invasion via JUP. In vivo gain of function of miR-195-5p reduced the numbers and sizes of tumors and significantly ameliorated the histopathological changes typical of CRC. In conclusion, our findings indicate a potential pharmacological target based on miR-195-5p replacement as a new therapeutic approach in CRC.
Triple Genetic Diagnosis in a Patient with Late-Onset Leukodystrophy and Mild Intellectual Disability
Authors: Pasquetti Domizia , Gazzellone Annalisa , Rossi Salvatore , Orteschi Daniela , L’Erario Federica Francesca , Concolino Paola , Minucci Angelo , Dionisi-Vici Carlo , Genuardi Maurizio , Silvestri Gabriella , Chiurazzi Pietro
Published: December 2023

We describe the complex case of a 44-year-old man with polycystic kidney disease, mild cognitive impairment, and tremors in the upper limbs. Brain MRI showed lesions compatible with leukodystrophy. The diagnostic process, which included clinical exome sequencing (CES) and chromosomal microarray analysis (CMA), revealed a triple diagnosis: autosomal dominant polycystic kidney disease (ADPKD) due to a pathogenic variant, c.2152C>T-p.(Gln718Ter), in the PKD1 gene; late-onset phenylketonuria due to the presence of two missense variants, c.842C>T-p.(Pro281Leu) and c.143T>C-p.(Leu48Ser) in the PAH gene; and a 915 Kb duplication on chromosome 15. Few patients with multiple concurrent genetic diagnoses are reported in the literature; in this ADPKD patient, genome-wide analysis allowed for the diagnosis of adult-onset phenylketonuria (which would have otherwise gone unnoticed) and a 15q11.2 duplication responsible for cognitive and behavioral impairment with incomplete penetrance. This case underlines the importance of clinical genetics for interpreting complex results obtained by genome-wide techniques, and for diagnosing concurrent late-onset monogenic conditions.
The Role of Sirtuin 6 in the Deacetylation of Histone Proteins as a Factor in the Progression of Neoplastic Disease
Authors: Baran Marzena , Miziak Paulina , Stepulak Andrzej , Cybulski Marek
Published: December 2023

SIRT6 is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, predominantly located in the nucleus, that is involved in the processes of histone modification, DNA repair, cell cycle regulation, and apoptosis. Disturbances in SIRT6 expression levels have been observed in the development and progression of various types of cancer. Therefore, it is important to better understand the role of SIRT6 in biochemical pathways and assign it specific biological functions. This review aims to summarize the role of SIRT6 in carcinogenesis and tumor development. A better understanding of the factors influencing SIRT6 expression and its biological role in carcinogenesis may help to develop novel anti-cancer therapeutic strategies. Moreover, we discuss the anti-cancer effects and mechanism of action of small molecule SIRT6 modulators (both activators and inhibitors) in different types of cancer.
Pathobiology of Cognitive Impairment in Parkinson Disease: Challenges and Outlooks
Authors: Jellinger Kurt A.
Published: December 2023

Cognitive impairment (CI) is a characteristic non-motor feature of Parkinson disease (PD) that poses a severe burden on the patients and caregivers, yet relatively little is known about its pathobiology. Cognitive deficits are evident throughout the course of PD, with around 25% of subtle cognitive decline and mild CI (MCI) at the time of diagnosis and up to 83% of patients developing dementia after 20 years. The heterogeneity of cognitive phenotypes suggests that a common neuropathological process, characterized by progressive degeneration of the dopaminergic striatonigral system and of many other neuronal systems, results not only in structural deficits but also extensive changes of functional neuronal network activities and neurotransmitter dysfunctions. Modern neuroimaging studies revealed multilocular cortical and subcortical atrophies and alterations in intrinsic neuronal connectivities. The decreased functional connectivity (FC) of the default mode network (DMN) in the bilateral prefrontal cortex is affected already before the development of clinical CI and in the absence of structural changes. Longitudinal cognitive decline is associated with frontostriatal and limbic affections, white matter microlesions and changes between multiple functional neuronal networks, including thalamo-insular, frontoparietal and attention networks, the cholinergic forebrain and the noradrenergic system. Superimposed Alzheimer-related (and other concomitant) pathologies due to interactions between α-synuclein, tau-protein and β-amyloid contribute to dementia pathogenesis in both PD and dementia with Lewy bodies (DLB). To further elucidate the interaction of the pathomechanisms responsible for CI in PD, well-designed longitudinal clinico-pathological studies are warranted that are supported by fluid and sophisticated imaging biomarkers as a basis for better early diagnosis and future disease-modifying therapies.
Storage Standards and Solutions, Data Storage, Sharing, and Structuring in Digital Health: A Brazilian Case Study
Authors: Oliveira Nicollas Rodrigues de , Santos Yago de Rezende dos , Mendes Ana Carolina Rocha , Barbosa Guilherme Nunes Nasseh , Oliveira Marcela Tuler de , Valle Rafael , Medeiros Dianne Scherly Varela , Mattos Diogo M. F.
Published: December 2023

The COVID-19 pandemic has highlighted the necessity for agile health services that enable reliable and secure information exchange, but achieving proper, private, and secure sharing of EMRs remains a challenge due to diverse data formats and fragmented records across multiple data silos, resulting in hindered coordination between healthcare teams, potential medical errors, and delays in patient care. While centralized EMR systems pose privacy risks and data format diversity complicates interoperability, blockchain technology offers a promising solution by providing decentralized storage, ensuring data integrity, enhancing access control, eliminating intermediaries, and increasing efficiency in healthcare. By focusing on a Brazilian case study, this paper explores the significance of EMR standards, security challenges, and blockchain-based approaches to promote interoperability and secure data sharing in the healthcare industry.
A Methodology for Integrating Hierarchical VMAP-Data Structures into an Ontology Using Semantically Represented Analyses
Authors: Spelten Philipp , Meyer Morten-Christian , Wagner Anna , Wolf Klaus , Reith Dirk
Published: December 2023

Integrating physical simulation data into data ecosystems challenges the compatibility and interoperability of data management tools. Semantic web technologies and relational databases mostly use other data types, such as measurement or manufacturing design data. Standardizing simulation data storage and harmonizing the data structures with other domains is still a challenge, as current standards such as the ISO standard STEP (ISO 10303 ”Standard for the Exchange of Product model data”) fail to bridge the gap between design and simulation data. This challenge requires new methods, such as ontologies, to rethink simulation results integration. This research describes a new software architecture and application methodology based on the industrial standard ”Virtual Material Modelling in Manufacturing” (VMAP). The architecture integrates large quantities of structured simulation data and their analyses into a semantic data structure. It is capable of providing data permeability from the global digital twin level to the detailed numerical values of data entries and even new key indicators in a three-step approach: It represents a file as an instance in a knowledge graph, queries the file’s metadata, and finds a semantically represented process that enables new metadata to be created and instantiated.
Diagonal Compression Tests on Unfired and Fired Masonry Wallettes Retrofitted with Textile-Reinforced Alkali-Activated Mortar
Authors: Arce Andres , Kapsalis Panagiotis , Papanicolaou Catherine G. , Triantafillou Thanasis C.
Published: December 2023

This paper discusses the integration of an alkali-activated mortar (AAM), based on industrial waste, into a novel composite material fit for structural upgrading purposes and rendered with high temperature endurance and a low CO2 footprint. The AAM combined with carbon fiber textiles form a new generation of sustainable inorganic matrix composites—that of textile-reinforced alkali-activated mortars (TRAAM). A test program was designed to assess the effectiveness of carbon TRAAM overlays in increasing the shear capacity of masonry wall specimens comprising solid clay bricks bonded with lime-based mortar and furnished with TRAAM jackets on both sides. The initial and the residual capacity of the reinforced walls were evaluated, the latter by performing diagonal compression tests after exposure to 300 °C and 550 °C. It was shown that TRAAM jacketing can increase the shear capacity of unfired masonry walls by 260% and 335% when a single or a double layer of textile is used, respectively. Rapid heating to temperatures up to 550 °C, one-hour-long steady-state heating, and natural cooling bore no visible thermal cracks on the specimens and had little effect on their residual capacity. Based on these results, the prospect of using TRAAM for retrofitting applications for fire-resilient structures seems very auspicious.
Acute Effects of Combining Whole-Body Electromyostimulation with Resistance Training in Active Women
Authors: Buonsenso Andrea , Centorbi Marco , Di Martino Giulia , Della Valle Carlo , Di Claudio Gloria , Di Fonza Domenico , Di Zazzo Erika , Calcagno Giuseppe , di Cagno Alessandra , Fiorilli Giovanni
Published: December 2023

Strength training elicits benefits both in performance and on a psychological level in women, such as increased muscle strength and improved self-esteem. Whole-body electromyostimulation (WB-EMS) could be a training strategy for enhancing muscular strength. The aim of this study was to assess the acute effects of a single session of WB-EMS superimposed over classic resistance training on isometric strength, endurance strength and flexibility. Furthermore, the safety of the protocol was assessed by monitoring the levels of creatine kinase (CK) 48 h after the training protocol was completed. Sixteen active women (aged 22.06 ± 1.88) were randomly assigned to an experimental group (EG) (n = 8) and a control group (CG) (n = 8). The EG performed four sets of 12 repetitions of three strength exercises with superimposed WB-EMS, while the CG performed the same protocol without WB-EMS. RM-ANOVA showed a significant time*group interaction on posterior kinetic chain extensors’ mean and peak strength in the EG (F(1,14) = 10.036; p = 0.007; and F(1,14) = 20.719; p < 0.001; respectively). A significant time*group interaction was found in the sit and reach test for the EG (F(1,14) = 10.362; p = 0.006). Finally, ANOVA performed on the CK levels showed no significant difference between the groups (F(1,14) = 0.715; p = 0.412). WB-EMS training led to an immediate improvement in strength performance and flexibility, and this protocol was shown to be safe in terms of CK levels, 48 h after completing the training protocol.
Inspection Operations and Hole Detection in Fish Net Cages through a Hybrid Underwater Intervention System Using Deep Learning Techniques
Authors: López-Barajas Salvador , Sanz Pedro J. , Marín-Prades Raúl , Gómez-Espinosa Alfonso , González-García Josué , Echagüe Juan
Published: December 2023

Net inspection in fish-farm cages is a daily task for divers. This task represents a high cost for fish farms and is a high-risk activity for human operators. The total inspection surface can be more than 1500 m2, which means that this activity is time-consuming. Taking into account the severe restrictions for human operators in such hostile underwater conditions, this activity represents a significant area for improvement. A platform for net inspection is proposed in this work. This platform includes a surface vehicle, a ground control station, and an underwater vehicle (BlueROV2 heavy) which incorporates artificial intelligence, trajectory control procedures, and the necessary communications. In this platform, computer vision was integrated, involving a convolutional neural network trained to predict the distance between the net and the robot. Additionally, an object detection algorithm was developed to recognize holes in the net. Furthermore, a simulation environment was established to evaluate the inspection trajectory algorithms. Tests were also conducted to evaluate how underwater wireless communications perform in this underwater scenario. Experimental results about the hole detection, net distance estimation, and the inspection trajectories demonstrated robustness, usability, and viability of the proposed methodology. The experimental validation took place in the CIRTESU tank, which has dimensions of 12 × 8 × 5 m, at Universitat Jaume I.
Unexpected Formation of the Iodobismuthate Salt (C14H15S2N2)2(C9H10SN)2[Bi4I16] upon Reaction of the Unsaturated Ligand Z-PySCH2CH=CHCH2SPy with BiI3
Authors: Essid Marwa , Hrizi Chakib , Ammar Salah , Khatyr Abderrahim , Knorr Michael , Schmidt Annika , Strohmann Carsten
Published: December 2023

The olefinic dithioether (Z)-1,4-bis(pyridin-2-ylthio)but-2-ene Z-PyS(CH2CH=CHCH2)SPy (L) was prepared by the treatment of cis-ClCH2CH=CHCH2Cl with in situ generated potassium pyridine-2-thiolate Py-SK and analyzed by IR and NMR spectroscopy. To investigate the chemistry of polynuclear iodobismuthate complexes, two equivalents of BiI3 were reacted with L in the MeOH solution to afford the anionic tetranuclear title compound (C14H15S2N2)2(C9H10SN)2[Bi4I116] with a N-protonated (Z)-1,4-bis(pyridin-2-ylthio)but-2-ene as a counterion. Compound 1 was characterized by IR and UV spectroscopy; the formation of a tetranuclear framework was ascertained by a single-crystal X-ray diffraction study performed at 100 K. Furthermore, an unusual Bi(III)-meditated cyclization of one Z-PyS(CH2CH=CHCH2)SPy ligand occurred, affording the bicyclic pyridinium salt 3-vinyl-2,3-dihydrothiazolo[3,2-a]pyridinium bearing a terminal vinyl group, compensating the second negative charge of the Bi4I164− cluster anion. The SCXRD characterization was completed by a Hirshfeld surface analysis, revealing some secondary interactions occurring in the crystal.
Crashworthiness of Additively Manufactured Auxetic Lattices: Repeated Impacts and Penetration Resistance
Authors: Franzosi Paolo , Colamartino Ivan , Giustina Alessandro , Anghileri Marco , Boniardi Marco
Published: December 2023

Auxetic materials have recently attracted interest in the field of crashworthiness thanks to their peculiar negative Poisson ratio, leading to densification under compression and potentially being the basis of superior behavior upon impact with respect to conventional cellular cores or standard solutions. However, the empirical demonstration of the applicability of auxeticity under impact is limited for most known geometries. As such, the present work strives to advance the investigation of the impact behavior of auxetic meta-materials: first by selecting and testing representative specimens, then by proceeding with an experimental and numerical study of repeated impact behavior and penetration resistance, and finally by proposing a new design of a metallic auxetic absorber optimized for additive manufacturing and targeted at high-performance crash applications.
Research on the Early-Age Cracking of Concrete Added with Magnesium Oxide under a Temperature Stress Test Machine
Authors: Li Zixuan , Chen Zheng , Li Junting , Xu Zhiwen , Wang Weilun
Published: December 2023

Concrete cracking is a significant issue in the global construction industry, and the restraint stress of concrete is a crucial contributing factor to early concrete cracking. The addition of magnesium oxide additive (MEA) to concrete is a method to enhance its crack resistance. In this paper, concrete specimens with four different contents of MEA were tested with a temperature stress testing machine. The deformation characteristics and mechanical properties of concrete with varying contents of MEA were investigated using both free deformation tests and fully constrained deformation tests. The prediction model for the early restrained stress of concrete was developed by integrating the stress relaxation phenomenon of concrete with models for autogenous shrinkage, temperature deformation, and elastic modulus. According to the results, (1) the thermal expansion coefficient exhibits a pattern of initially increasing and subsequently decreasing with the increasing ratio of MEA; (2) the addition of 3% and 8% MEA can offset 23% and 35.1% of the concrete’s self-shrinkage, respectively. Nevertheless, when the added MEA content is 5%, the self-shrinkage of concrete increases by 6%; (3) the addition of 3–8% MEA can result in a 0.5–1.67 times increase in the maximum expansion stress of concrete, as well as a 0.5–0.95 times increase in cracking stress; (4) as the MEA content continues to increase, the stress relaxation level of concrete also increases. In comparison to concrete mixed without MEA, the maximum increase in the stress relaxation level of concrete is 65.5%, thereby enhancing the concrete’s anti-cracking ability. However, when the MEA dosage reaches a certain threshold, the stress relaxation enhancement brought about by the addition of MEA will no longer be significant; (5) when compared to the experimental data, the established model of early-age constraint stress accurately predicts the tensile constraint stress of concrete.
Impact of Stone Powder Content on Corrosion Resistance in Reinforced Concrete under Stray Current and Chloride Interactions
Authors: Zhang Yuanzhu , Zhang Xuanming , Jin Fan , Zhao Xiuyi
Published: December 2023

Manufactured sand, known for its artificial production, eco-friendliness, cost-effectiveness, and sustainability, serves as an excellent alternative to natural sand. Stone powder content plays a crucial role in determining the performance of manufactured sand, significantly enhancing concrete compaction and its ability to withstand environmental degradation. This study aims to explore the feasibility of using environmentally and economically advantageous manufactured sand in the construction of subway tunnel segments in coastal areas. It investigates the impact of stone powder content on the corrosion resistance of concrete made with manufactured sand under the combined influence of chloride salts and stray currents. The analysis covers corrosion tendencies, the post-rusting performance of reinforcement bars, the resistance of concrete to chloride transport, and microstructure properties, which are assessed through electron microscope scanning and mercury compression testing. The findings indicate that the corrosion resistance of manufactured sand concrete generally surpasses that of river sand concrete. Furthermore, stone powder content within the 3–8% range leads to a denser concrete microstructure, reduced porosity, lower free-chloride ion levels, increased polarization resistance of post-corrosion reinforcements, lower corrosion current density, and reduced mass loss of reinforcing bars. This research provides valuable theoretical support for promoting the use of environmentally friendly manufactured sand concrete in subway construction projects.
Nano-Scale Engineering of Heterojunction for Alkaline Water Electrolysis
Authors: Chen Yao , Xu Zhenbo , Chen George Zheng
Published: December 2023

Alkaline water electrolysis is promising for low-cost and scalable hydrogen production. Renewable energy-driven alkaline water electrolysis requires highly effective electrocatalysts for the hydrogen evolution reaction (HER) and the oxygen evolution reaction (OER). However, the most active electrocatalysts show orders of magnitude lower performance in alkaline electrolytes than that in acidic ones. To improve such catalysts, heterojunction engineering has been exploited as the most efficient strategy to overcome the activity limitations of the single component in the catalyst. In this review, the basic knowledge of alkaline water electrolysis and the catalytic mechanisms of heterojunctions are introduced. In the HER mechanisms, the ensemble effect emphasizes the multi-sites of different components to accelerate the various intermedium reactions, while the electronic effect refers to the d-band center theory associated with the adsorption and desorption energies of the intermediate products and catalyst. For the OER with multi-electron transfer, a scaling relation was established: the free energy difference between HOO* and HO* is 3.2 eV, which can be overcome by electrocatalysts with heterojunctions. The development of electrocatalysts with heterojunctions are summarized. Typically, Ni(OH)2/Pt, Ni/NiN3 and MoP/MoS2 are HER electrocatalysts, while Ir/Co(OH)2, NiFe(OH)x/FeS and Co9S8/Ni3S2 are OER ones. Last but not the least, the trend of future research is discussed, from an industry perspective, in terms of decreasing the number of noble metals, achieving more stable heterojunctions for longer service, adopting new craft technologies such as 3D printing and exploring revolutionary alternate alkaline water electrolysis.
Research on Damage Caused by Carbon-Fiber-Reinforced Polymer Robotic Drilling Based on Digital Image Correlation and Industrial Computed Tomography
Authors: Shi Feng , Yang Yi , Sun Nianjun , Du Zhaocai , Zhang Chen , Zhao Dongjie
Published: December 2023

In order to enhance application scenarios and increase the proportion of industrial robots in the field of drilling composites, the damage caused by carbon-fiber-reinforced polymer robotic drilling is studied. The shortcomings of the existing damage evaluation factors are analyzed, and new damage evaluation factors for carbon-fiber-reinforced polymer laminates made of unidirectional prepreg are proposed. A robot and a brad-and-spur drill were used to drill carbon-fiber-reinforced polymer laminates to study the influence of the process parameters on robotic drilling damage. Digital image correlation equipment and industrial computed tomography were used to study the formation process and the damage forms of the hole on the exit side with different process parameters. The test results show that delamination and tearing are significantly affected by the feed rate and spindle speed, while burrs are less affected by the cutting parameters. Appropriately increasing the spindle speed and reducing the feed rate are beneficial to reducing the comprehensive damage factor and improving the hole quality. To avoid hole scrapping caused by a large amount of damage, it is suggested that the robotic drilling parameters should be controlled at a spindle speed higher than 8000 rpm and a feed rate lower than 360 mm/min.
First-Order Sparse TSK Nonstationary Fuzzy Neural Network Based on the Mean Shift Algorithm and the Group Lasso Regularization
Authors: Zhang Bingjie , Wang Jian , Gong Xiaoling , Shi Zhanglei , Zhang Chao , Zhang Kai , El-Alfy El-Sayed M. , Ablameyko Sergey V.
Published: December 2023

Nonstationary fuzzy inference systems (NFIS) are able to tackle uncertainties and avoid the difficulty of type-reduction operation. Combining NFIS and neural network, a first-order sparse TSK nonstationary fuzzy neural network (SNFNN-1) is proposed in this paper to improve the interpretability/translatability of neural networks and the self-learning ability of fuzzy rules/sets. The whole architecture of SNFNN-1 can be considered as an integrated model of multiple sub-networks with a variation in center, variation in width or variation in noise. Thus, it is able to model both “intraexpert” and “interexpert” variability. There are two techniques adopted in this network: the Mean Shift-based fuzzy partition and the Group Lasso-based rule selection, which can adaptively generate a suitable number of clusters and select important fuzzy rules, respectively. Quantitative experiments on six UCI datasets demonstrate the effectiveness and robustness of the proposed model.
A Group Theoretic Approach to Cyclic Cubic Fields
Authors: Aouissi Siham , Mayer Daniel C.
Published: December 2023

Let (kμ)μ=14 be a quartet of cyclic cubic number fields sharing a common conductor c=pqr divisible by exactly three prime(power)s, p,q,r. For those components of the quartet whose 3-class group Cl3(kμ)≃(Z/3Z)2 is elementary bicyclic, the automorphism group M=Gal(F32(kμ)/kμ) of the maximal metabelian unramified 3-extension of kμ is determined by conditions for cubic residue symbols between p,q,r and for ambiguous principal ideals in subfields of the common absolute 3-genus field k* of all kμ. With the aid of the relation rank d2(M), it is decided whether M coincides with the Galois group G=Gal(F3∞(kμ)/kμ) of the maximal unramified pro-3-extension of kμ.
Impact of COVID-19 Pandemic and Associated Restrictions on Vitamin D Status in a Large Cohort of Italian Children and Adolescents
Authors: Antonucci Roberto , Vacca Nadia , Biasia Beatrice , Locci Cristian , Dore Maria Pina , Pes Giovanni Mario , Bitti Angela
Published: December 2023

Background and Objectives: Vitamin D is synthesized in the skin upon sunlight exposure, showing variations with season and latitude. We aimed to investigate the influence of age, sex, and season on vitamin D status in a large pediatric cohort during the COVID-19 pandemic period and the corresponding pre-pandemic period. Materials and Methods: Retrospective data concerning subjects aged < 18 years were extracted anonymously from the large database of a reference laboratory hospital (Sassari, Northern Sardinia, Italy). Serum 25-hydroxyvitamin D [25(OH)D] levels measured during the pre-pandemic period (1 March 2018 to 30 September 2019) were compared with those detected during the pandemic period (1 March 2020 to 30 September 2021). Results: A total of 2317 samples from subjects aged < 18 years were included in the analysis, 1303 (47.9% females) of which were collected in the pre-pandemic period and 1014 (51.3% females) in the pandemic period. No significant differences in 25(OH)D levels were found between the two periods, whereas, in children aged < 2 years, levels were higher than those in children aged 11–16 years (p < 0.05). Monthly levels of 25(OH)D between pre-pandemic and pandemic periods did not differ, although significant differences were detected across months (p < 0.0001). Similarly, 25(OH)D values did not differ significantly between males and females in both periods. Marked seasonal variations were observed in males and females across all age groups. Conclusions: Serum vitamin D levels and their season-related variations were not significantly affected by the COVID-19 pandemic and associated restrictions in a large cohort of Italian children and adolescents.
Prevalence of JC Polyomavirus in Patients with Neuroinvasive Disease of Unknown Etiology in Croatia
Authors: Vilibic-Cavlek Tatjana , Bogdanic Maja , Peric Tajana , Radmanic Leona , Antolasic Ljiljana , Milasincic Ljiljana , Zidovec-Lepej Snjezana
Published: December 2023

Background and Objectives: John Cunningham polyomavirus (JCPyV) is a highly prevalent virus in the human population. The prevalence of JCPyV in patients with central nervous system disorders has not been examined extensively. The aim of this study was to analyze the prevalence of JCPyV DNA/antibodies in patients with neuroinvasive diseases (NID) of unknown etiology. Materials and Methods: The study included 132 patients with NID (febrile headache, meningitis, encephalitis) tested from January 2021 to December 2022. The control group consisted of 47 asymptomatic individuals. In patients with NID, serum and cerebrospinal fluid (CSF) samples were collected in the acute phase of the disease. CSF samples were tested for JCPyV DNA (PCR), while serum samples were tested for JCPyV IgG antibodies (ELISA). In controls, serum samples were tested for JCPyV IgG antibodies (ELISA). Results: JCPyV DNA was not detected in any of the CSF samples from patients with NID. JCPyV IgG antibodies were detected in 88.6% of patients and 74.5% of controls (p < 0.001). In the patients’ group, a significant difference in the IgG prevalence was observed between males (94.6%) and females (81.0%). In addition, significant differences in the seropositivity between age groups were found. The lowest seroprevalence (28.6%) was in patients less than 20 years, followed by a sharp increase in the 20–29-year group (69.2%), after which the seroprevalence remained stable (90.0–94.1%) in patients up to 69 years. All patients older than 70 years were JCPyV IgG-seropositive. No significant difference in the seroprevalence was found in patients presenting with febrile headache (81.6%), meningitis (93.3%), or meningoencephalitis (91.3%). No difference in the seropositivity between genders was found in controls. Although the seropositivity steadily increased in older participants, these differences were not significant. Analyzing the JCPyV antibody levels in patients with NID, the median antibody titers differed significantly between groups, ranging from 248 AU/mL (younger age groups) to 400 AU/mL (older age groups). Conclusions: Higher seroprevalence in the patients’ group highlights the need to further investigate the possible association of JCPyV and NID.
Metabolic Research in Aquatic Animal Nutrition, Physiology and Disease
Authors: He Yan , Tan Qingsong , Wang Qingchao
Published: December 2023

Aquaculture provides a significant amount of high-quality protein for human consumption and is one of the most efficient protein production industries [...]
Increasing the Light Extraction Efficiency of Organic Light-Emitting Devices by Electrochemically Corroded Patterned Substrates
Authors: Wang Yang , Li Zhonghao , Bai Yu , Wang Yingzhi
Published: December 2023

A substrate with microstructure can increase the light extraction efficiency of OLEDs. However, the present preparation methods for micro- and nanostructures are not suited for broad-area manufacturing. In this research, we suggested an electrochemical etching approach to patterning Si substrates and effectively generated a vast area of micro-/nanostructures on the surface of Si. We created OLEDs using this patterned substrate. It was discovered through this study that when the current density is 100 mA/cm2, the brightness increases by 1.67 times and the efficiency increases by 1.43 times, over a planar equivalent. In the future, this electrochemical etching process for patterned silicon substrates might give rise to a new approach to the large-scale manufacture of microstructured silicon substrates.
Genesis of the Large-Scale Kamado Magnesite Deposit on the Tibetan Plateau
Authors: Yu Xuhui , Hu Guyue , Chen Yuchuan , Xu Ying , Chen Han , Wang Denghong , Huang Fan , You Shuisheng , Liu Haiyong , He Liang , Li Yubin
Published: December 2023

Lacustrine strata-bound magnesite deposits associated with Alpine-type ultramafic rocks are hydrothermal in origin. The magnesite ores of the Kamado deposit are unconformably underlain by mid-Jurassic marine carbonate and ultramafic rocks of the Bangong-Nujiang ophiolite suite and are in fault contact with hanging wall rocks composed of siliceous sinter. Three types of cryptocrystalline magnesite ores can be identified in Kamado: (1) strata-bound massive magnesites, representing the main ore type in the upper part; (2) banded ores in the lower part; and (3) some vein and stockwork ore in the ultramafic wall rocks. Integrated scanning electron microscopy, C–O isotope analysis, and geochemical analyses were carried out on the Kamado deposit. The results indicate that: (1) the orebody is composed of magnesite, with accessory minerals of aragonite, opal, and chromite; (2) the siliceous sinter and relatively high B (32.0–68.1 ppm) and Li (14.7–23.4 ppm) contents of the magnesite ores reflect long-term spring activity in Kamado; (3) the light carbon (δ13CV-PDB: −4.7 ± 0.3‰ to −4.1 ± 0.6‰) and oxygen isotopic compositions (δ18OV-SMOW: +12.3 ± 0.3 to +16.3 ± 0.1‰) of the stockwork ores in the foot wall rocks indicated that the carbon in fractures in the ultramafic rocks is from a mixture of marine carbonate and oxidized organic-rich sedimentary rocks, reflecting a typical “Kraubath-type” magnesite deposit; and (4) the relatively heavy carbon isotopic (δ13CV-PDB: +8.7 ± 0.4‰ to +8.8 ± 0.3‰) composition of the banded magnesite ores in the lower segment may have formed from heavy CO2 generated by anaerobic fermentation in the lakebed. Additionally, the carbon isotopic (δ13CV-PDB: +7.3 ± 0.3‰ to +7.7 ± 0.7‰) composition of the massive magnesite ores in the upper segment indicates a decline in the participation of anaerobic fermentation. As this economically valuable deposit is of the strata-bound massive ore type, Kamado can be classified as a lacustrine hydrothermal-sedimentary magnesite deposit, formed by continuous spring activities under salt lakes on the Tibetan Plateau, with the Mg mainly being contributed by nearby ultramafic rocks and the carbon mainly being sourced from atmosphere-lake water exchange, with minor amounts from marine carbonate strata.
Mineral-like Synthetic Compounds Stabilized under Hydrothermal Conditions: X-ray Diffraction Study and Comparative Crystal Chemistry
Authors: Kiriukhina Galina , Yakubovich Olga , Verchenko Polina , Volkov Anatoly , Shvanskaya Larisa , Dimitrova Olga , Simonov Sergey
Published: December 2023

Under hydrothermal conditions emulating natural hydrothermalites, three oxo-salts with sodium and transition metal cations were obtained in the form of single crystals. Their compositions and crystal structures were studied using scanning electron microscopy, microprobe X-ray spectral analysis, and X-ray single-crystal diffraction. The sodium cobalt silicate, i.e., Na2CoSiO4, a structural analog of the mineral liberite, is well known as an ionic conductor. Its crystal structure consists of a framework derived from β-tridymite, built using the Co- and Si-centered tetrahedra sharing vertices. The sodium oxocuprate phosphate chloride Na2Cu3O(Cu0.8Na0.2)(PO4)2Cl belongs to a group of compounds, including fumarolic minerals, characterized by the presence of oxo-centered pyroxene-like chains in their structures. The crystal structure of mineralogically probable sodium vanadium phosphate hydroxide (Na3V(OH)(HPO4)(PO4)) is based on chains built using octahedra centered by magnetically active V3+. Magnetic susceptibility measurements indicate an antiferromagnetic arrangement of V3+ ions and no transition to an ordered state up to 2 K.
Lipase-Assisted Synthesis of Alkyl Stearates: Optimization by Taguchi Design of Experiments and Application as Defoamers
Authors: Olvera-Ureña Enoch , Lopez-Tellez Jorge , Vizueto M. Monserrat , Hidalgo-Ledezma J. Guadalupe , Martinez-Quiroz Baltazar , Rodriguez Jose A.
Published: December 2023

The present work proposes the optimization of enzymatic synthesis of alkyl stearates using stearic acid, alkyl alcohols (C1-OH, C2-OH, C4-OH, C8-OH and C16-OH) and Candida rugosa lipase by a L9 (34) Taguchi-type design of experiments. Four variables were evaluated (reaction time, temperature, kU of lipase and alcohol:stearic acid molar ratio), ensuring that all variables were critical. In optimal conditions, five stearates were obtained with conversions > 90%. The obtained products were characterized by nuclear magnetic resonance (NMR). Additionally, the defoaming capacity of the five stearates was evaluated, obtaining better performance for the compound synthesized from C8-OH alcohol.
Applying Cognitive Load Theory to eLearning of Crafts
Authors: Partarakis Nikolaos , Zabulis Xenophon
Published: December 2023

Craft education and training are important for preserving cultural heritage and fostering artisanal skills. However, the pedagogical challenges in this domain are numerous. This research paper presents a comprehensive framework for applying Cognitive Load Theory to enhance craft education and training via eLearning platforms. In this study, practical guidelines based on CLT principles are provided to optimize the instructional design and content delivery. These guidelines scaffold craft learning experiences within eLearning platforms and encompass strategies to manage cognitive load, promote active learning, and facilitate gradual transition. Subsequently, the paper details the implementation of these guidelines within a popular eLearning platform, Moodle, emphasizing its adaptability and utility for craft education. It discusses the customization of Moodle courses to align with the cognitive load management principles, providing a practical blueprint for educators and instructional designers. The research culminates in a case study, wherein the guidelines are applied to a craft eLearning course using Moodle.
Vitamin D Supplementation at a Dose of 10 µg/Day in Institutionalized Children with Severe Motor and Intellectual Disabilities
Authors: Sato Yota , Kamei Atsushi , Endo Fumie , Matsuyama Sakura , Toda Hiroyuki , Kasai Takeo
Published: December 2023

Vitamin D (VD) deficiency can lead to health-related consequences. This study determined the effects of VD administration in VD-deficient children with severe motor and intellectual disabilities (SMID). Twenty-eight subjects were included. Among them, 25 subjects with parental consent for VD administration were given 10 µg/day (400 IU/day) of VD in April 2021. Serum 25-hydroxyvitamin D [25(OH)D] levels were measured at least 30 days after the start of VD administration. The total VD intake, serum 25(OH)D levels, and ultraviolet (UV) exposure before the blood tests were investigated. The results showed that the median serum 25(OH)D levels were 8.7 ng/mL (4.3–17.2) and 24.0 ng/mL (7.8–39 ng/mL) from March to May in 2020 and 2021, respectively. Among the 25 subjects, 22 with UV exposure had >20 ng/mL serum 25(OH)D level, and 2 without UV exposure had <20 ng/mL serum 25(OH)D level. Three subjects who did not receive VD supplementation had <20 ng/mL serum 25(OH)D level. Taken together, VD supplementation (10 µg/day) is effective in children with SMID in institutional care. Moreover, it may be sufficient for children with UV exposure, but not for those without.
Intranasal Drug Delivery by Nanotechnology: Advances in and Challenges for Alzheimer’s Disease Management
Authors: Dighe Sayali , Jog Sunil , Momin Munira , Sawarkar Sujata , Omri Abdelwahab
Published: December 2023

Alzheimer’s disease, a progressive neurodegenerative condition, is characterized by a gradual decline in cognitive functions. Current treatment approaches primarily involve the administration of medications through oral, parenteral, and transdermal routes, aiming to improve cognitive function and alleviate symptoms. However, these treatments face limitations, such as low bioavailability and inadequate permeation. Alternative invasive methods, while explored, often entail discomfort and require specialized assistance. Therefore, the development of a non-invasive and efficient delivery system is crucial. Intranasal delivery has emerged as a potential solution, although it is constrained by the unique conditions of the nasal cavity. An innovative approach involves the use of nano-carriers based on nanotechnology for intranasal delivery. This strategy has the potential to overcome current limitations by providing enhanced bioavailability, improved permeation, effective traversal of the blood–brain barrier, extended retention within the body, and precise targeting of the brain. The comprehensive review focuses on the advancements in designing various types of nano-carriers, including polymeric nanoparticles, metal nanoparticles, lipid nanoparticles, liposomes, nanoemulsions, Quantum dots, and dendrimers. These nano-carriers are specifically tailored for the intranasal delivery of therapeutic agents aimed at combatting Alzheimer’s disease. In summary, the development and utilization of intranasal delivery systems based on nanotechnology show significant potential in surmounting the constraints of current Alzheimer’s disease treatment strategies. Nevertheless, it is essential to acknowledge regulatory as well as toxicity concerns associated with this route; meticulous consideration is required when engineering a carrier. This comprehensive review underscores the potential to revolutionize Alzheimer’s disease management and highlights the importance of addressing regulatory considerations for safe and effective implementations. Embracing this strategy could lead to substantial advancements in the field of Alzheimer’s disease treatment.
Fine Structure of High-Energy Pulses from a Stimulated Brillouin Scattering-Assisted Q-Switch Tm-Doped Fiber Laser
Authors: Kamynin Vladimir A. , Filatova Serafima A. , Mullanurov Timur I. , Cheban Maksim D. , Wolf Alexey A. , Korobko Dmitry A. , Fotiadi Andrei A. , Tsvetkov Vladimir B.
Published: December 2023

We have demonstrated a simple all-fiber thulium (Tm) laser Q-switched by stimulated Brillouin scattering (SBS). The maximum output pulse energy was 80 μJ. This allowed us to generate a broadband spectrum directly at the laser outputs. For the first time, we measured the fine structure of the output pulses with a resolution of less than 100 ps. It was found that the SBS Q-switched laser is capable of generating bunches of picosecond pulses. The effect of modulation instability on the pulse decay is discussed. The potential application of the investigated laser radiation for producing destructive effects on soft biological tissues has been demonstrated.