bluuebunny/crossref_metadata_2025 huggingface dataset:
The PI3K-Akt pathway is a multifaceted regulator of the macrophage response to diverse group B Streptococcus isolates
Authors: De-Leon-Lopez Yadira S. , Thompson Michelle E. , Kean Jessica J. , Flaherty Rebecca A.
Published: October 2023

Group B Streptococcus (GBS), also known as Streptococcus agalactiae, is a common member of the microbial flora in healthy individuals. However, problems may arise when GBS-colonized mothers become pregnant. GBS may be transferred from a colonized mother to her newborn or developing fetus, which may result in complications such as miscarriage, pre-term birth, meningitis, pneumonia, or sepsis. Macrophages play an especially important role in the fetal and newborn response to GBS due to the limited development of the adaptive immune system early in life. The goal of this study was to expand what is currently known about how GBS manipulates macrophage cell signaling to evade the immune system and cause disease. To this end, we investigated whether the PI3K-Akt pathway was involved in several key aspects of the macrophage response to GBS. We explored whether certain GBS strains, such as sequence type (ST)-17 strains, rely on this pathway for the more rapid macrophage uptake they induce compared to other GBS strains. Our findings suggest that this pathway is, indeed, important for macrophage uptake of GBS. Consistent with these findings, we used immunofluorescence microscopy to demonstrate that more virulent strains of GBS induce more actin projections in macrophages than less virulent strains. Additionally, we explored whether PI3K-Akt signaling impacted the ability of GBS to survive within macrophages after phagocytosis and whether this pathway influenced the survival rate of macrophages themselves following GBS infection. The PI3K-Akt pathway was found to promote the survival of both macrophages and intracellular GBS following infection. We also observed that inhibition of the PI3K-Akt pathway significantly reduced GBS-mediated activation of NFκB, which is a key regulator of cell survival and inflammatory responses. Overall, these insights into strain-dependent GBS-mediated manipulation of the PI3K-Akt pathway and its downstream targets in infected macrophages may provide new insights for the development of diagnostic and therapeutic tools to combat severe GBS disease.
Digital inclusion for social inclusion. Case study on digital literacy
Authors: Méndez-Domínguez Paula , Carbonero Muñoz Domingo , Raya Díez Esther , Castillo De Mesa Joaquín
Published: October 2023

The aim of this research is to identify the differences in access to technologies and digital skills of the population according to their socioeconomic characteristics and to analyse the opportunities offered by new emerging learning environments to promote the social inclusion of vulnerable groups. The digital divide is defined as inequality in the access, use, or impact of information and communication technologies (ICT), and, to address it, it is necessary to build on the conceptual frameworks developed in research to date. This study seeks to 1) identify the main difficulties in digital access and skills and 2) explore what the adoption, design, development, and adaptation of emerging learning technologies mean for the most disadvantaged groups. A quantitative, research design was used. The results obtained show that there are differences in digital skills and access according to education and income level. Different statistical analyses were used, such as non-parametric tests and tests of association between variables. The survey was carried out on a proportional sample of 400 people in La Rioja (Spain). Data was collected through online and face-to-face surveys. A quantitative approach was implemented in the first phase. In the second phase, students of the Social Work degree programme, social work professionals, and users of the Senior Citizens' Center (older adults) were included. The qualitative research is based on the development of digital literacy, which seeks to test and provide new insights into the use of innovative learning-teaching methodologies, with digital materials (micro videos), to promote the use and knowledge of ICT as a means of bridging social (as well as digital) divides.
Unveiling cognitive aspects and accuracy of diagnostic judgments in physical education teachers assessing different tasks in gymnastics
Authors: Leukel Christian , Leuders Timo , Bessi Flavio , Loibl Katharina
Published: October 2023

Introduction

Diagnostics is an essential part of teachers’ profession. We investigated judgment accuracy and cognitive processes underlying judgment formation in physical education teachers who observed tasks in gymnastics, and compared teachers with gymnastics trainers as a reference group.

Methods

Teachers and trainers judged performance of prepuberal students in gymnastics, namely students exercising squat vault, underswing, and handstand. To investigate cognitive processes of judgment formation, participants were asked to structure the movements via event segmentation as well as to explain their judgments. All teachers and trainers had experience in working with prepuberal children similar to those they observed in this experiment, and the teachers completed a gymnastics class during their studies.

Results

Judgment accuracy (with reference to judgments made by expert trainers) was found to be significantly lower in teachers compared to trainers (p < 0.001). Moreover, agreement on the ratings among teachers was lower than among trainers. Agreement about the temporal structuring of the tasks from event segmentation was lower among teachers than among trainers (p < 0.05). When explaining their ratings, trainers referred more often than teachers to kinematic features of the task that were relevant to the judgments.

Discussion

We discuss these findings in context of the teachers’ task to perform accurate judgments. For suggestions on teacher training, we particularly emphasize the relevance of implementing knowledge about kinematic features of the tasks and student errors into real-life scenarios resembling the complex skill of making accurate judgments in the physical education classroom.
Does income inequality have a heterogeneous effect on carbon emissions between developed and developing countries? Evidence from simultaneous quantile regression
Authors: Che Cheng , Li Suhui , Yin Qi , Li Qing , Geng Xin , Zheng Huixian
Published: October 2023

The sustainable development process faces two major challenges: income inequality and carbon emissions. However, there is no definitive conclusion regarding the relationship between income inequality and carbon emissions. In addition, for developed and developing countries, the impact might vary. From an international perspective, the present study examines the correlation between income inequality and carbon emissions utilizing panel data from 65 countries (32 developed and 33 developing countries) spanning from 1990 to 2019. The simultaneous quantile regression (SQR) results show that the impact of income inequality on carbon emissions varies slightly between countries with various income levels. The positive impact of income inequality on carbon emissions is significant for both developed and developing countries, indicating that an uneven income distribution can worsen environmental quality, but the impact is more significant for developed countries. Therefore, it is integral for the global world to take measures to alleviate the income gap to achieve Sustainable Development Goals (SDGs). In addition to income growth, developed countries also need to focus on income equity to reach green development.
Paradigms unfolded – developing, validating, and evaluating the Medical Education e-Professionalism framework from a philosophical perspective
Authors: Guraya Shaista Salman , Harkin Denis W. , Yusoff Muhamad Saiful Bahri , Guraya Salman Yousuf
Published: October 2023

In order to ensure a strong research design, literature stresses the adoption of a research paradigm that is consistent with the researcher’s beliefs about the nature of reality. In this article we provide an overview of research paradigm choices in relation to the creation of a Medical Education e-Professionalism (MEeP) framework discussing the research design, research methods, data collection and analysis to enhance the transparency of our previously published research. The MEeP framework was conceived to help Health Care Professionals (HCPs) safeguard the construct of professionalism in the digital context. This entire process was heavily informed by wider readings and deliberations of published literature on e-professionalism. Although the MEeP framework research journey has been published, the paradigms approach was not discussed in any detail. Considering that one of the duties of medical educator is to balance the service and science by bringing the theoretical underpinnings of one’s research to public attention and scrutiny so as to nullify the notion of ‘weak’ research. We were compelled to unfold this paradigm story of the MEeP framework in a detailed manner. In an effort to make our research both robust and effective, this study portrays a philosophical approach to guide future research designs and methodological choices by detailing our rationale for pragmatism as a choice of paradigm.
Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation
Authors: Vion Charlotte , Brambati Mathilde , Da Costa Grégory , Richard Tristan , Marullo Philippe
Published: October 2023

This study explored the intracellular metabolic variations between 17 strains of Saccharomyces cerevisiae belonging to two different genetic populations: flor and wine yeasts, in the context of alcoholic fermentation. These two populations are closely related as they share the same ecological niche but display distinct genetic characteristics. A protocol was developed for intracellular metabolites extraction and 1H-NMR analysis. This methodology allowed us to identify and quantify 21 intracellular metabolites at two different fermentation steps: the exponential and stationary phases. This work provided evidence of significant differences in the abundance of intracellular metabolites, which are strain- and time-dependent, thus revealing complex interactions. Moreover, the differences in abundance appeared to be correlated with life-history traits such as average cell size and specific glycolytic flux, which revealed unsuspected phenotypic correlations between metabolite load and fermentation activity.
Effects of long-term metal exposure on the structure and co-occurrence patterns of the oral microbiota of residents around a mining area
Authors: Pei Shuwei , Feng Lu , Zhang Yonghua , Liu Jiangyun , Li Jia , Zheng Qiwen , Liu Xingrong , Luo Bin , Ruan Ye , Li Huan , Hu Weigang , Niu Jingping , Tian Tian
Published: October 2023

Objectives

The aim of our study was to investigate the impact of long-term exposure to heavy metals on the microbiome of the buccal mucosa, to unveil the link between environmental contamination and the oral microbial ecosystem, and to comprehend its potential health implications.

Methods

Subjects were divided into two groups: the exposure group and the control group. We collected samples of buccal mucosa, soil, and blood, and conducted microbial diversity analysis on both groups of oral samples using 16S rRNA gene sequencing. The concentrations of heavy metals in blood and soil samples were also determined. Additionally, microbial networks were constructed for the purpose of topological analysis.

Results

Due to long-term exposure to heavy metals, the relative abundance of Rhodococcus, Delftia, Fusobacterium, and Peptostreptococcus increased, while the abundance of Streptococcus, Gemella, Prevotella, Granulicatella, and Porphyromonas decreased. The concentrations of heavy metals in the blood (Pb, Cd, Hg, and Mo) were associated with the growth of Rhodococcus, Delftia, Porphyromonas, and Gemella. In addition, the relative abundances of some pathogenic bacteria, such as Streptococcus anginosus, S. gordonii, and S. mutans, were found to be enriched in the exposure group. Compared to the exposure group network, the control group network had a greater number of nodes, modules, interactive species, and keystone taxa. Module hubs and connectors in the control group converted into peripherals in the exposure group, indicating that keystone taxa changed. Metals in the blood (Pb, Cd, Hg, and Mo) were drivers of the microbial network of the buccal mucosa, which can have adverse effects on the network, thus providing conditions for the occurrence of certain diseases.

Conclusion

Long-term exposure to multiple metals perturbs normal bacterial communities in the buccal mucosa of residents in contaminated areas. This exposure reduces the complexity and stability of the microbial network and increases the risk of developing various diseases.
Research hotspots and trends of transcranial magnetic stimulation in Parkinson’s disease: a bibliometric analysis
Authors: Wei Yi-xin , Tu Liang-dan , He Lin , Qiu Yi-tong , Su Wei , Zhang Li , Ma Run-ting , Gao Qiang
Published: October 2023

Background

Transcranial magnetic stimulation (TMS), as a non-invasive neuromodulation technique, has been widely used in the treatment of Parkinson’s disease (PD). The increasing application of TMS has promoted an increasing number of clinical studies. In this paper, a bibliometric analysis of existing studies was conducted to reveal current research hotspots and guide future research directions.

Method

Relevant articles and reviews were obtained from the Science Citation Index Expanded of Web of Science Core Collection database. Data related to publications, countries, institutions, authors, journals, citations, and keywords in the studies included in the review were systematically analyzed using VOSviewer 1.6.18 and Citespace 6.2.4 software.

Result

A total of 1,894 papers on the topic of TMS in PD between 1991 and 2022 were analyzed and visualized to identify research hotspots and trends in the field. The number of annual publications in this field of study has increased gradually over the past 30 years, with the number of annual publications peaking in 2022 (n = 150). In terms of publications and total citations, countries, institutions, and authors from North America and Western Europe were found to make significant contributions to the field. The current hotspot focuses on the effectiveness of TMS for PD in different stimulation modes or different stimulated brain regions. The keyword analysis indicates that the latest research is oriented to the mechanism study of TMS for motor symptoms in PD, and the non-motor symptoms are also receiving more attention.

Conclusion

Our study offers insights into the current hotspots and emerging trends of TMS in the rehabilitation of PD. These findings may serve as a guide for future research and the application of TMS for PD.
RGMa and Neogenin control dendritic spine morphogenesis via WAVE Regulatory Complex-mediated actin remodeling
Authors: Sempert Kai , Shohayeb Belal , Lanoue Vanessa , O’Brien Elizabeth A. , Flores Cecilia , Cooper Helen M.
Published: October 2023

Structural plasticity, the ability of dendritic spines to change their volume in response to synaptic stimulation, is an essential determinant of synaptic strength and long-term potentiation (LTP), the proposed cellular substrate for learning and memory. Branched actin polymerization is a major force driving spine enlargement and sustains structural plasticity. The WAVE Regulatory Complex (WRC), a pivotal branched actin regulator, controls spine morphology and therefore structural plasticity. However, the molecular mechanisms that govern WRC activation during spine enlargement are largely unknown. Here we identify a critical role for Neogenin and its ligand RGMa (Repulsive Guidance Molecule a) in promoting spine enlargement through the activation of WRC-mediated branched actin remodeling. We demonstrate that Neogenin regulates WRC activity by binding to the highly conserved Cyfip/Abi binding pocket within the WRC. We find that after Neogenin or RGMa depletion, the proportions of filopodia and immature thin spines are dramatically increased, and the number of mature mushroom spines concomitantly decreased. Wildtype Neogenin, but not Neogenin bearing mutations in the Cyfip/Abi binding motif, is able to rescue the spine enlargement defect. Furthermore, Neogenin depletion inhibits actin polymerization in the spine head, an effect that is not restored by the mutant. We conclude that RGMa and Neogenin are critical modulators of WRC-mediated branched actin polymerization promoting spine enlargement. This study also provides mechanistic insight into Neogenin’s emerging role in LTP induction.
A scoping review and critical evaluation of the methodological quality of clinical practice guidelines on nutrition in the preconception
Authors: Ancira-Moreno Mónica , Burrola-Méndez Soraya , Muñoz-Manrique Cinthya , Omaña-Guzmán Isabel , Hoyos-Loya Elizabeth , Trejo-Domínguez Alejandra , Hernández-Cordero Sonia , Mazariegos Mónica , Smith Natalia , Tavano-Colaizzi Loredana , Mier-Cabrera Jennifer , Avendaño-Álvarez Fermín , Espino y Sosa Salvador , Muciño-Sandoval Karla , Ibarra-González Lizeth , Medina-Avilés Cristina
Published: October 2023

Introduction

Clinical practice guidelines (CPGs) contain recommendations for specific clinical circumstances, including maternal malnutrition. This study aimed to identify the CPGs that provide recommendations for preventing, diagnosing, and treating women’s malnutrition. Additionally, we sought to assess the methodological quality using the Appraisal of Guidelines for Research and Evaluation (AGREE II) instrument.

Methods

An online search for CPGs was performed, looking for those that contained lifestyle and nutritional recommendations to prevent, diagnose and treat malnutrition in women during the preconception period using PubMed and different websites. The reviewers utilized the AGREE II instrument to appraise the quality of the CPGs. We defined high-quality guidelines with a final score of > 70%.

Results

The titles and abstracts from 30 guidelines were screened for inclusion, of which 20 guidelines were fully reviewed for quality assessment. The overall quality assessment of CPGs was 73%, and only 55% reached a high-quality classification. The domains in the guidelines classified as high-quality had the highest scores in “Scope and Purpose” and “Clarity of Presentation” with a median of 98.5 and 93%, respectively.

Discussion

Further assessment is needed to improve the quality of the guidelines, which is an opportunity to strengthen them, especially in the domains with the lowest scores.
Advancing pharmacogenetic testing in a tertiary hospital: a retrospective analysis after 10 years of activity
Authors: Stewart Stefan , Dodero-Anillo Jose Manuel , Guijarro-Eguinoa Javier , Arias Pedro , Gómez López De Las Huertas Arturo , Seco-Meseguer Enrique , García-García Irene , Ramírez García Elena , Rodríguez-Antolín Carlos , Carcas Antonio J. , Rodriguez-Novoa Sonia , Rosas-Alonso Rocio , Borobia Alberto M.
Published: October 2023

The field of pharmacogenetics (PGx) holds great promise in advancing personalized medicine by adapting treatments based on individual genetic profiles. Despite its benefits, there are still economic, ethical and institutional barriers that hinder its implementation in our healthcare environment. A retrospective analysis approach of anonymized data sourced from electronic health records was performed, encompassing a diverse patient population and evaluating key parameters such as prescribing patterns and test results, to assess the impact of pharmacogenetic testing. A head-to-head comparison with previously published activity results within the same pharmacogenetic laboratory was also conducted to contrast the progress made after 10 years. The analysis revealed significant utilization of pharmacogenetic testing in daily clinical practice, with 1,145 pharmacogenetic tests performed over a 1-year period and showing a 35% growth rate increase over time. Of the 17 different medical departments that sought PGx tests, the Oncology department accounted for the highest number, representing 58.47% of all genotyped patients. A total of 1,000 PGx tests were requested for individuals susceptible to receive a dose modification based on genotype, and 76 individuals received a genotype-guided dose adjustment. This study presents a comprehensive descriptive analysis of real-world data obtained from a public tertiary hospital laboratory specialized in pharmacogenetic testing, and presents data that strongly endorse the integration of pharmacogenetic testing into everyday clinical practice.
Legume-grass mixtures increase forage yield by improving soil quality in different ecological regions of the Qinghai-Tibet Plateau
Authors: Luo Feng , Liu Wenhui , Mi Wenbo , Ma Xiang , Liu KaiQiang , Ju Zeliang , Li Wen
Published: October 2023

Introduction

Information on the relationship between soil quality and forage yield of legume-grass mixtures in different ecological regions can guide decision-making to achieve eco-friendly and sustainable pasture production. This study’s objective was to assess the effects of different cropping systems on soil physical properties, nitrogen fractions, enzyme activities, and forage yield and determine suitable legume-grass mixtures for different ecoregions.

Methods

Oats (Avena sativa L.), forage peas (Pisum sativum L.), common vetch (Vicia sativa L.), and fava beans (Vicia faba L.) were grown in monocultures and mixtures (YS: oats and forage peas; YJ: oats and common vetch; YC: oats and fava beans) in three ecological regions (HZ: Huangshui Valley; GN: Sanjiangyuan District; MY: Qilian Mountains Basin) in a split-plot design.

Results

The results showed that the forage yield decreased with increasing altitude, with an order of GN (3203 m a.s.l.; YH 8.89 t·ha-1) < HZ (2661 m; YH 9.38 t·ha-1) < MY (2513m; YH 9.78 t·ha-1). Meanwhile, the forage yield was higher for mixed crops than for single crops in all ecological regions. In the 0-10 cm soil layer, the contents of total nitrogen (TN), microbial biomass nitrogen (MBN), soil organic matter (SOM), soluble organic nitrogen (SON), urease (UE), nitrate reductase (NR), sucrase (SC), and bacterial community alpha diversity, as well as relative abundance of dominant bacteria, were higher for mixed crops than for oats unicast. In addition, soil physical properties, nitrogen fractions, and enzyme activities varied in a wider range in the 0-10 cm soil layer than in the 10-20 cm layer, with larger values in the surface layer than in the subsurface layer. MBN, SON, UE, SC and catalase (CAT) were significantly and positively correlated with forage yield (P < 0.05). Ammonium nitrogen (ANN), nitrate nitrogen (NN), SOM and cropping systems (R) were significantly and positively correlated with Shannon and bacterial community (P < 0.05). The highest yields in the three ecological regions were 13.00 t·ha-1 for YS in MY, 10.59 t·ha-1 for YC in GN, and 10.63 t·ha-1 for YS in HZ.

Discussion

We recommend planting oats and forage peas in the Qilian Mountains Basin, oats and fava beans in the Sanjiangyuan District, and oats and forage peas in Huangshui valley. Our results provide new insights into eco-friendly, sustainable, and cost-effective forage production in the Qinghai Alpine Region in China.
Using an episodic specificity induction to improve children’s future thinking
Authors: Tanguay Annick F. N. , Gardam Olivia , Archibald Jane , Ayson Gladys , Atance Cristina M.
Published: October 2023

Episodic future thinking (EFT) is the ability to subjectively pre-experience a specific future event. Future-oriented cognition in young children positively predicts physical health and financial status later in life. Can EFT be improved in children, even temporarily? Developmental research emphasizes the importance of thinking about one’s own near future to enhance EFT, whereas research in adults suggests benefits reside in constructing a richly detailed event. We bridged the two perspectives to examine whether a procedure, the “episodic specificity induction” (ESI), could be adapted to encourage an episodic mode of thinking in children, benefitting performance on a variety of subsequent EFT tasks. The present study implemented a child-friendly ESI in which children mentally simulated a future event and were probed for specific details about it. We randomly assigned 66 children aged 6 and 7 years to one of two conditions: (1) ESI, in which children imagined “having breakfast tomorrow” in detail, describing surroundings, people, and actions, or (2) a Control condition (i.e., no construction), in which children simply viewed and described a picture of another child having breakfast. Children then completed a series of future thinking tasks assessing prospective memory, recollection/imagination of events, delay of gratification, and planning. Our ESI was successful in promoting the construction of a detailed event, and subsequently increasing the number of details of recollected and imagined events on an outcome task as compared to a control condition. Nonetheless, the effect of ESI was smaller than expected – a finding that fits with recent work suggesting that such interventions may be too cognitively taxing for young children and/or that benefits may hinge on further development in episodic processes. We discuss possible modifications to the induction and implications for EFT amelioration in young children.
Deep neural network for prediction of diet quality among doctors and nurses in North China during the COVID-19 pandemic
Authors: Wang Qihe , Chu Haiyun , Li Huzhong , Li Congyan , Li Shuting , Fang Haiqin , Liang Dong , Deng Taotao , Li Jinliang , Liu Aidong
Published: October 2023

Objective

The COVID-19 pandemic has placed unprecedented pressure on front-line healthcare workers, leading to poor health status, especially diet quality. This study aimed to develop a diet quality prediction model and determine the predictive effects of personality traits, socioeconomic status, lifestyles, and individual and working conditions on diet quality among doctors and nurses during the COVID-19 pandemic.

Methods

A total of 5,013 doctors and nurses from thirty-nine COVID-19 designated hospitals provided valid responses in north China in 2022. Participants’ data related to social-demographic characteristics, lifestyles, sleep quality, personality traits, burnout, work-related conflicts, and diet quality were collected with questionnaires. Deep Neural Network (DNN) was applied to develop a diet quality prediction model among doctors and nurses during the COVID-19 pandemic.

Results

The mean score of diet quality was 46.14 ± 15.08; specifically, the mean scores for variety, adequacy, moderation, and overall balance were 14.33 ± 3.65, 17.99 ± 5.73, 9.41 ± 7.33, and 4.41 ± 2.98, respectively. The current study developed a DNN model with a 21–30–28-1 network framework for diet quality prediction. The DNN model achieved high prediction efficacy, and values of R2, MAE, MSE, and RMSE were 0.928, 0.048, 0.004, and 0.065, respectively. Among doctors and nurses in north China, the top five predictors in the diet quality prediction model were BMI, poor sleep quality, work–family conflict, negative emotional eating, and nutrition knowledge.

Conclusion

During the COVID-19 pandemic, poor diet quality is prevalent among doctors and nurses in north China. Machine learning models can provide an automated identification mechanism for the prediction of diet quality. This study suggests that integrated interventions can be a promising approach to improving diet quality among doctors and nurses, particularly weight management, sleep quality improvement, work-family balance, decreased emotional eating, and increased nutrition knowledge.
The role of anxiety in patients with hereditary angioedema during oral treatment: a narrative review
Authors: Rosa Alessio , Franco Rocco , Miranda Michele , Casella Sergio , D’Amico Cesare , Fiorillo Luca , Cervino Gabriele
Published: October 2023

Objective

The present study investigated the clinical potential of managing anxiety during dental procedures to reduce acute attacks in patients with hereditary angioedema (HAE). HAE is a rare disease, little known to physicians and dentists, but with an increased hospitalization rate over the years. HAE is due to a deficiency/dysfunction of the C1 esterase inhibitor, leading to increased vascular permeability. Recommendations for HAE management include long-term and short-term prophylaxis and treatment of acute attacks, but the importance of anxiety control is underestimated.

Materials and methods

The authors reviewed the literature to provide the scientific community with an overview of possible protocols for managing anxiety in dental practice and their effectiveness. Management can be used in prosthetics, periodontal and implant surgery, endodontics, and oral surgery.

Discussions

Our analysis shows that although there are few articles in the indexed literature, protocols for managing anxiety in HAE patients in dentistry will become increasingly prevalent in the daily clinical practice of dentists due to its benefits.

Conclusions

The benefits and better control of intraoperative complications and risks may lead clinicians to use sedation, assessment, or anxiety control techniques in daily clinical practice to reduce such attacks. Clinical relevance: This study suggests that controlling and managing anxiety can help prevent and reduce acute angioedema attacks.
Generation and characterization of a monoclonal antibody against an African swine fever virus protein encoded by the A137R gene
Authors: Embury-Hyatt Carissa , Moffat Estella , Zhmendak Dmytro , Erdelyan Cassidy N. G. , Collignon Brad , Goonewardene Kalhari , Ambagala Aruna , Yang Ming
Published: October 2023

The ongoing African swine fever (ASF) pandemic continues to have a major impact on global pork production and trade. Since ASF cannot be distinguished from other swine hemorrhagic fevers clinically, ASF-specific laboratory diagnosis is critical. Thus ASF virus (ASFV)-specific monoclonal antibodies (mAbs) are critical for the development of laboratory diagnostics. In this study, we report one ASFV-specific mAb, F88ASF-55, that was generated and characterized. This mAb recognizes the ASFV A137R-encoded protein (pA137R). Epitope mapping results revealed a highly conserved linear epitope recognized by this mAb, corresponding to amino acids 111–125 of pA137R. We explored the potential use of this mAb in diagnostic applications. Using F88ASF-55 as the detection antibody, six ASFV strains were detected in an enzyme-linked immunosorbent assay (ELISA) with low background. In immunohistochemistry (IHC) assays, this mAb specifically recognized ASFV antigens in the submandibular lymph nodes of animals experimentally infected with different ASFV strains. Although not all ASFV genotypes were tested in this study, based on the conserved ASFV epitope targeted by F88ASF-55, it has the potential to detect multiple ASFV genotypes. In conclusion, this newly generated ASFV pA137R-specific mAb has potential value in ASF diagnostic tool development. It can be used in ELISA, IHC, and possibly-immunochromatographic strip assays for ASFV detection. It also suggests that pA137R may be a good target for diagnostic assays to detect ASFV infection.
Simulations of Compression Ramp Shock Wave/Turbulent Boundary Layer Interaction Controlled via Steady Jets at High Reynolds Number
Authors: Dai Tingkai , Zhang Bo
Published: October 2023

Shock wave/turbulent boundary layer interaction (SBLI) is one of the most common physical phenomena in transonic wing and supersonic aircraft. In this study, the compression ramp SBLI (CR-SBLI) was simulated at a 24° corner at Mach 2.84 using the open-source OpenFOAM improved delayed detached eddy simulation (IDDES) turbulence model and the “Rescaling and Recycling” method at high Reynolds number 1.57×106. The results of the control effect of the jet vortex generator on CR-SBLI showed that the jet array can effectively reduce the length of the separation zone. The simulation results of different jet parameters are obtained. With the increasing jet angle, the reduction in the length of the separation zone first increased and then decreased. In this work, when the jet angle was 60°, the location of the separation point was x/δ=−1.48, which was smaller than other jet angles. The different distances of the jet array also had a great influence. When the distance between the jet and the corner djet=70 mm, the location of the separation point x/δ=−1.48 was smaller than that when djet=65/60 mm. A closer distance between the jet hole and the corner caused the vortex structures to squeeze each other, preventing the formation of a complete vortex structure. On the other hand, when the jet was farther away, the vortex structures could separate effectively before reaching the shock wave, resulting in a better inhibition of SBLI. The simulation primarily focused on exploring the effects of the jet angle and distance, and we obtained the jet parameters that provided the best control effect, effectively reducing the length of the CR-SBLI separation zone.
An Extension Algorithm of Regional Eigenvalue Assignment Controller Design for Nonlinear Systems
Authors: Arıcan Ahmet Çağrı , Çopur Engin Hasan , Inalhan Gokhan , Salamci Metin Uymaz
Published: October 2023

This paper provides a new method to nonlinear control theory, which is developed from the eigenvalue assignment method. The main purpose of this method is to locate the pointwise eigenvalues of the linear-like structure built by freezing the nonlinear systems at a given time instant in a desired disk region. Since the control requirements for the transient response characteristics are the major constraints on the selection of the disk centre and radius, two different update algorithms are also developed to reshape the disk region by changing the disk centre and radius at each time step. The effectiveness of the proposed methods is tested in both simulations and experiments. A validated three-DOF laboratory helicopter is used for experiments.
A Novel Approach for Assessing Technical Grade and Quality of Lambda-Cyhalothrin and Acetamiprid in Insecticides Used in Agricultural Systems by HPLC Technique in Southern Benin
Authors: Tossou Eric , Tepa-Yotto Ghislain , Tchigossou Genevieve M. , Soglo Murielle F. , Mbokou Serge Foukmeniok , Bougna Tchoumi Honorine Hortense , Bokonon-Ganta Aimé H. , Tamò Manuele , Djouaka Rousseau
Published: October 2023

In Benin, synthetic insecticides are the main pest control option used by farmers to protect and enhance their production. However, failures to control the target pests are often observed after application and may be related to agricultural practices or insecticide quality. The present work was designed to assess a rapid, simple, and reliable analytical method for detecting and quantifying the most commonly used insecticides (λ-cyhalothrin and acetamiprid) in Benin. The analytical standard technical grade separation was performed by gradient reversed-phase high-performance liquid chromatography on a C18 stationary-phase column. The mobile phase consisted of a mixture of acetonitrile/water using a gradient flow. The flow rates were 1 and 1.4 mL·min−1 for λ-cyhalothrin and acetamiprid, respectively. The analysis times were 15 and 20 min, with retention times of 2.35 and 7.94 min for λ-cyhalothrin and acetamiprid, respectively. Results reveal that most of the surveyed farmers were not educated (70% < Primary School Certificate) and were men (95%). Of the main insecticides applied by farmers, λ-cyhalothrin and acetamiprid were found to be the most technical-grade ones. Furthermore, the analysis of insecticides showed that the concentrations obtained in our study often differed from the ones mentioned on insecticide labels. The proposed method is useful for quantifying insecticides in various technical and commercial formulations with little interference from additives.
The Research on Ultrasonic Vibration Amplitudes in Ti6Al4V DED Additive Manufacturing
Authors: Liu Wei , Zhang Zhenchao , Li Suli , Xu Chao
Published: October 2023

Ultrasonic-assisted Ti6Al4V Directed Energy Deposition (DED) additive manufacturing technology can improve the problem of uneven microstructure caused by laser heating and sudden cooling of the molten pool. In this paper, the numerical analysis and experimental verification methods were adopted. The influencing factors, such as the cavitations’ effect, sound flow enhancement effect, and sound flow thermal effect related to the ultrasonic assistance in the molten pool, were analyzed. After equating the energy of the ultrasound, the model of additive manufacturing was introduced in the form of a heat source. The temperature gradient changes during the solidification process of the molten pool with the addition of ultrasound assistance and the effect of ultrasonic vibration during the manufacturing process on its deposited state and microstructure of solution-aged formed parts were studied. The results showed that when the wire feeding rate is 5 mm/s and the laser scanning speed is 5 mm/s, the optimal laser power is 1000 W~1100 W, corresponding to the optimal ultrasonic amplitude of 120 μm. Then, by comparing the temperature field with the same amplitude of 0 μm (i.e., no ultrasonic vibration) and the microstructure of the formed parts, it was verified that ultrasonic vibration facilitates fluid flow in the molten pool, which could lead to a more uniform temperature distribution. This optimized approach not only enhances the understanding of the process but also contributes significantly to the advancement of related research endeavors.
Effects of Antimicrobial Administration Route on Growth and Antimicrobial Resistance in Weaned Piglets
Authors: Lee Seong-Won , Jung Chang-Min , Do Kyung-Hyo , Lee Wan-Kyu , Seo Kwang-Won
Published: October 2023

This study aimed to determine how the route of antimicrobial administration affected the growth performance of weaned piglets. Additionally, we aimed to investigate potential differences between antimicrobial resistance developed by antimicrobials administered orally through drinking water, and those administered through feed, in weaned piglets. The research was undertaken on a farm housing 500 sows and involved 150 weaned piglets at 21 days of age. These piglets were evenly distributed into three groups of equal size: water, feed, and control. Antimicrobials were administered through drinking water and feed in the water and feed groups, respectively, while the control group received no antimicrobial treatment. The observation of piglets continued until they reached 70 days of age. The feed conversion ratio in the water group (1.7 ± 0.78) was significantly higher than in the control (2.4 ± 1.77) and feed (2.7 ± 1.68) groups. Additionally, the route of administration did not affect antimicrobial resistance rates. Based on these results, it can be inferred that administering antimicrobials through drinking water is advantageous for pig farming.
Antimicrobial Use in On-Farm Hatching Systems vs. Traditional Hatching Systems: A Case Study
Authors: Jerab Julia G. , Chantziaras Ilias , Van Limbergen Tommy , Van Erum Johan , Boel Filip , Hoeven Erik , Dewulf Jeroen
Published: October 2023

On-farm hatching is a relatively new method in the broiler industry, in which fertilized broiler eggs are transported to the farms at the stage of 17–19 days of incubation. Once hatched, the broiler chicks have direct access to feed and water. Previous studies have shown on-farm hatching to increase animal welfare and intestinal development. However, no studies have yet aimed to quantify and compare the antimicrobial use in on-farm hatched flocks with that of traditionally hatched flocks. In this study, information on antimicrobial use (AMU) was collected from 211 Belgian conventional broiler farms, including data from 2244 traditionally hatched flocks and 227 on-farm (NestBorn) hatched flocks. On-farm hatched flocks had significantly (p < 0.001) more antimicrobial-free flocks (n = 109, 48.01%) compared to traditional flocks (n = 271, 12.08%) and a 44% lower (p < 0.01) treatment incidence (TI) at flock level (TI 8.40 vs. TI 15.13). Overall, the farms using traditional hatching had 5.6 times (95% CI 3.6–8.7) higher odds to use antimicrobials than the farms using on-farm hatching. Treated on-farm hatched flocks received three times less lincomycin-spectinomycin (linco-spectin) and less (routine) treatments at the start of the production round. However, both traditional and on-farm flocks experienced outbreaks later in the production round. These results show that on-farm hatching can contribute to the reduction in antimicrobial use in conventional broiler production.
Molecular Typing and Resistance Profile of Acinetobacter baumannii Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project
Authors: Agodi Antonella , Montineri Arturo , Manuele Rosa , Noto Paola , Carpinteri Giuseppe , Castiglione Giacomo , Grassi Patrizia , Lazzara Antonio , Mattaliano Anna Rita , Granvillano Giuseppa , La Mastra Claudia , La Rosa Maria Clara , Maugeri Andrea , Barchitta Martina
Published: October 2023

Due to the COVID-19 pandemic, there has been a shift in focus towards controlling the spread of SARS-CoV-2, which has resulted in the neglect of traditional programs aimed at preventing healthcare-associated infections and combating antimicrobial resistance. The present work aims to characterize the colonization or infection with Acinetobacter baumannii of COVID-19 patients and to identify any clonality between different isolates. Specifically, data and resistance profiles of A. baumannii isolates were prospectively collected from patients recruited by the EPIRADIOCLINF project. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were used for molecular typing. Overall, we analyzed 64 isolates of A. baumannii from 48 COVID-19 patients. According to our analysis, we have identified the spread of a clonally related isolate, referred to as B. The PFGE pattern B includes four subtypes: B1 (consisting of 37 strains), B2 (11), B3 (5), and B4 (2). Furthermore, in the isolates that were examined using MLST, the most observed sequence type was ST/281. In terms of resistance profiles, 59 out of the total isolates (92.2%) were found to be resistant to gentamicin, carbapenems, ciprofloxacin, and tobramycin. The isolation and identification of A. baumannii from COVID-19 patients, along with the high levels of transmission observed within the hospital setting, highlight the urgent need for the implementation of effective prevention and containment strategies.
Postbiotic Preparation of Lacticaseibacillus rhamnosus GG against Diarrhea and Oxidative Stress Induced by Spike Protein of SARS-CoV-2 in Human Enterocytes
Authors: Poeta Marco , Cioffi Valentina , Tarallo Antonietta , Damiano Carla , Lo Vecchio Andrea , Bruzzese Eugenia , Parenti Giancarlo , Guarino Alfredo
Published: October 2023

The Spike protein of SARS-CoV-2 acts as an enterotoxin able to induce chloride secretion and production of reactive oxygen species (ROS), involved in diarrhea pathogenesis. L. rhamnosus GG (LGG) is recommended in pediatric acute gastroenteritis guidelines as a therapy independent of infectious etiology. We tested a postbiotic preparation of LGG (mLGG) in an in vitro model of COVID-associated diarrhea. Caco-2 cell monolayers mounted in Ussing chambers were exposed to Spike protein, and electrical parameters of secretory effect (Isc and TEER) were recorded in the Ussing chambers system. Oxidative stress was analyzed by measuring ROS production (DCFH-DA), GSH levels (DNTB), and lipid peroxidation (TBARS). Experiments were repeated after mLGG pretreatment of cells. The Isc increase induced by Spike was consistent with the secretory diarrhea pattern, which was dependent on oxidative stress defined by a 2-fold increase in ROS production and lipid peroxidation and variation in glutathione levels. mLGG pretreatment significantly reduced the secretory effect (p = 0.002) and oxidative stress, namely ROS (p < 0.001), lipid peroxidation (p < 0.001), and glutathione level changes (p < 0.001). LGG counteracts Spike-induced diarrhea by inhibiting the enterotoxic effect and oxidative stress. The LGG efficacy in the form of a postbiotic depends on metabolites secreted in the medium with antioxidant properties similar to NAC. Because SARS-CoV-2 is an enteric pathogen, the efficacy of LGG independent of etiology in the treatment of acute gastroenteritis is confirmed by our data.
Microalgae as a Sustainable Source of Antioxidants in Animal Nutrition, Health and Livestock Development
Authors: Mavrommatis Alexandros , Tsiplakou Eleni , Zerva Anastasia , Pantiora Panagiota D. , Georgakis Nikolaos D. , Tsintzou Georgia P. , Madesis Panagiotis , Labrou Nikolaos E.
Published: October 2023

Microalgae are a renewable and sustainable source of bioactive compounds, such as essential amino acids, polyunsaturated fatty acids, and antioxidant compounds, that have been documented to have beneficial effects on nutrition and health. Among these natural products, the demand for natural antioxidants, as an alternative to synthetic antioxidants, has increased. The antioxidant activity of microalgae significantly varies between species and depends on growth conditions. In the last decade, microalgae have been explored in livestock animals as feed additives with the aim of improving both animals’ health and performance as well as product quality and the environmental impact of livestock. These findings are highly dependent on the composition of microalgae strain and their amount in the diet. The use of carbohydrate-active enzymes can increase nutrient bioavailability as a consequence of recalcitrant microalgae cell wall degradation, making it a promising strategy for monogastric nutrition for improving livestock productivity. The use of microalgae as an alternative to conventional feedstuffs is becoming increasingly important due to food–feed competition, land degradation, water deprivation, and climate change. However, the cost-effective production and use of microalgae is a major challenge in the near future, and their cultivation technology should be improved by reducing production costs, thus increasing profitability.
Neuroprotective Properties of Berberine: Molecular Mechanisms and Clinical Implications
Authors: Tian Erjie , Sharma Gaurav , Dai Chongshan
Published: October 2023

Berberine (BBR), an isoquinoline alkaloid natural product, is isolated primarily from Coptis chinensis and other Berberis plants. BBR possesses various bioactivities, including antioxidant, anti-inflammation, anticancer, immune-regulation, and antimicrobial activities. Growing scientific evidence underscores BBR’s substantial neuroprotective potential, prompting increased interest and scrutiny. In this comprehensive review, we elucidate the neuroprotective attributes of BBR, delineate the underlying molecular mechanisms, and assess its clinical safety and efficacy. The multifaceted molecular mechanisms responsible for BBR’s neuroprotection encompass the attenuation of oxidative stress, mitigation of inflammatory responses, inhibition of apoptotic pathways, facilitation of autophagic processes, and modulation of CYP450 enzyme activities, neurotransmitter levels, and gut microbiota composition. Furthermore, BBR engages numerous signaling pathways, including the PI3K/Akt, NF-κB, AMPK, CREB, Nrf2, and MAPK pathways, to confer its neuroprotective effects. This comprehensive review aims to provide a substantial knowledge base, stimulate broader scientific discourse, and facilitate advancements in the application of BBR for neuroprotection.