bluuebunny/crossref_metadata_2025 huggingface dataset:
Feature-Learning-Based Printed Circuit Board Inspection via Speeded-Up Robust Features and Random Forest
Authors: Yuk Eun Hye , Park Seung Hwan , Park Cheong-Sool , Baek Jun-Geol
Published: June 2018

With the coming of the 4th industrial revolution era, manufacturers produce high-tech products. As the production process is refined, inspection technologies become more important. Specifically, the inspection of a printed circuit board (PCB), which is an indispensable part of electronic products, is an essential step to improve the quality of the process and yield. Image processing techniques are utilized for inspection, but there are limitations because the backgrounds of images are different and the kinds of defects increase. In order to overcome these limitations, methods based on machine learning have been used recently. These methods can inspect without a normal image by learning fault patterns. Therefore, this paper proposes a method can detect various types of defects using machine learning. The proposed method first extracts features through speeded-up robust features (SURF), then learns the fault pattern and calculates probabilities. After that, we generate a weighted kernel density estimation (WKDE) map weighted by the probabilities to consider the density of the features. Because the probability of the WKDE map can detect an area where the defects are concentrated, it improves the performance of the inspection. To verify the proposed method, we apply the method to PCB images and confirm the performance of the method.
Clinico-Pathological Importance of TGF-β/Phospho-Smad Signaling during Human Hepatic Fibrocarcinogenesis
Authors: Yoshida Katsunori , Matsuzaki Koichi , Murata Miki , Yamaguchi Takashi , Suwa Kanehiko , Okazaki Kazuichi
Published: June 2018

Chronic viral hepatitis is a global public health problem, with approximately 570 million persons chronically infected. Hepatitis B and C viruses increase the risk of morbidity and mortality from liver cirrhosis, hepatocellular carcinoma (HCC), and extrahepatic complications that develop. Hepatitis virus infection induces transforming growth factor (TGF)-β, which influences microenvironments within the infected liver. TGF-β promotes liver fibrosis by up-regulating extracellular matrix production by hepatic stellate cells. TGF-β is also up-regulated in patients with HCC, in whom it contributes importantly to bringing about a favorable microenvironment for tumor growth. Thus, TGF-β is thought to be a major factor regulating liver fibrosis and carcinogenesis. Since TGF-β carries out regulatory signaling by influencing the phosphorylation of Smads, we have generated several kinds of phospho-specific antibodies to Smad2/3. Using these, we have identified three types of phospohorylated forms: COOH-terminally phosphorylated Smad2/3 (pSmad2C and pSmad3C), linker phosphorylated Smad2/3 (pSmad2L and pSmad3L), and dually phosphorylated Smad3 (pSmad2L/C and pSmad3L/C). TGF-β-mediated pSmad2/3C signaling terminates cell proliferation; on the other hand, cytokine-induced pSmad3L signaling accelerates cell proliferation and promotes fibrogenesis. This review addresses TGF-β/Smad signal transduction in chronic liver injuries and carcinogenic processes. We also discuss the reversibility of Smad signaling after antiviral therapy.
Electrochemically Obtained TiO2/CuxOy Nanotube Arrays Presenting a Photocatalytic Response in Processes of Pollutants Degradation and Bacteria Inactivation in Aqueous Phase
Authors: Kozak Magda , Mazierski Paweł , Żebrowska Joanna , Kobylański Marek , Klimczuk Tomasz , Lisowski Wojciech , Trykowski Grzegorz , Nowaczyk Grzegorz , Zaleska-Medynska Adriana
Published: June 2018

TiO2/CuxOy nanotube (NT) arrays were synthesized using the anodization method in the presence of ethylene glycol and different parameters applied. The presence, morphology, and chemical character of the obtained structures was characterized using a variety of methods—SEM (scanning electron microscopy), XPS (X-ray photoelectron spectroscopy), XRD (X-ray crystallography), PL (photoluminescence), and EDX (energy-dispersive X-ray spectroscopy). A p-n mixed oxide heterojunction of Ti-Cu was created with a proved response to the visible light range and the stable form that were in contact with Ti. TiO2/CuxOy NTs presented the appearance of both Cu2O (mainly) and CuO components influencing the dimensions of the NTs (1.1–1.3 µm). Additionally, changes in voltage have been proven to affect the NTs’ length, which reached a value of 3.5 µm for Ti90Cu10_50V. Degradation of phenol in the aqueous phase was observed in 16% of Ti85Cu15_30V after 1 h of visible light irradiation (λ > 420 nm). Scavenger tests for phenol degradation process in presence of NT samples exposed the responsibility of superoxide radicals for degradation of organic compounds in Vis light region. Inactivation of bacteria strains Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), and Clostridium sp. in presence of obtained TiO2/CuxOy NT photocatalysts, and Vis light has been studied showing a great improvement in inactivation efficiency with a response rate of 97% inactivation for E. coli and 98% for Clostridium sp. in 60 min. Evidently, TEM (transmission electron microscopy) images confirmed the bacteria cells’ damage.
AC Ship Microgrids: Control and Power Management Optimization
Authors: Al-Falahi Monaaf D. A. , Tarasiuk Tomasz , Jayasinghe Shantha Gamini , Jin Zheming , Enshaei Hossein , Guerrero Josep M.
Published: June 2018

At sea, the electrical power system of a ship can be considered as an islanded microgrid. When connected to shore power at berth, the same power system acts as a grid connected microgrid or an extension of the grid. Therefore, ship microgrids show some resemblance to terrestrial microgrids. Nevertheless, due to the presence of large dynamic loads, such as electric propulsion loads, keeping the voltage and frequency within a permissible range and ensuring the continuity of supply are more challenging in ship microgrids. Moreover, with the growing demand for emission reductions and fuel efficiency improvements, alternative energy sources and energy storage technologies are becoming popular in ship microgrids. In this context, the integration of multiple energy sources and storage systems in ship microgrids requires an efficient power management system (PMS). These challenging environments and trends demand advanced control and power management solutions that are customized for ship microgrids. This paper presents a review on recent developments of control technologies and power management strategies proposed for AC ship microgrids.
Three-Dimensional Peridynamic Model for Predicting Fracture Evolution during the Lithiation Process
Authors: Wang Hanlin , Oterkus Erkan , Oterkus Selda
Published: June 2018

Due to its large electric capacity, silicon has become one of the most promising electrode materials for lithium ion batteries. However, silicon undergoes large volumetric expansion and material stiffness reduction during the charging process. This can lead to fracture and failure of lithium-ion batteries. Damage formation and evolution inside the electrode are influenced by the lithium ion concentration and electrode material. High stress gradients induced by heterogeneous deformation can lead to massive migration of lithium ions towards high geometrical singularity regions, such as crack edge regions, which increases the lithium ion concentration. Fully coupled mechanical diffusion equations are important in describing the mechanics of this problem. In this study, the three-dimensional peridynamic theory is presented to solve the coupled field problem. In addition, the newly developed peridynamic differential operator concept is utilized to convert partial differential equations into peridynamic form for the diffusion equation. Spherical and cylindrical shaped energy storage structures with different pre-existing penny-shaped cracks are considered to demonstrate the capability of the developed framework. It is shown that peridynamic theory is a suitable tool for predicting crack evolution during the lithiation process.
Spatial and Temporal Variation of NDVI in Response to Climate Change and the Implication for Carbon Dynamics in Nepal
Authors: Baniya Binod , Tang Qiuhong , Huang Zhongwei , Sun Siao , Techato Kua-anan
Published: June 2018

Nepal is a country of contrast, with varying altitude, climate and vegetation from the top of high mountains to the tropical forest in low lands. The terrestrial vegetation has rapidly been altered by climate change in Nepal. The spatial and temporal evolution of vegetation and its linkage to climatic variables were analyzed using the Normalized Difference Vegetation Index (NDVI) obtained from Advanced Very High Resolution Radiometer (AVHRR) sensors. A linear regression model and Sen’s slope method were used to estimate NDVI trends and the Pearson correlation between NDVI and climatic variable, i.e., temperature and precipitation were calculated to identify the role of climate in vegetation changes. The carbon dynamics were also measured using a biomass carbon density estimation model. Results showed that NDVI experienced an overall increasing trend in Nepal from 1982–2015. The NDVI significantly increased at the rate of 0.0008 year−1 (p < 0.05) with seasonal variation of 0.0004 year−1, p > 0.05; 0.0007 year−1, p < 0.05; 0.0008 year−1, p < 0.05 and 0.0007 year−1, p > 0.05 in winter, pre-monsoon, monsoon and post-monsoon seasons, respectively. The NDVI relative change ratio (RCR) was 6.29% during last 34 years in Nepal. The correlation between NDVI and temperature was significantly positive (r = 0.36, p = 0.03), but there was a negative correlation with precipitation (r = −0.21, p = 0.28). Altogether, 82.20% of the study areas showed a positive correlation with temperature in which 34.97% was significant and 69.23% of the area had a negative correlation (16.35% significant, p < 0.05) with precipitation. In addition, NDVI-based carbon estimation showed that Nepal’s forest total carbon stock is 685.45 × 106 t C (i.e., an average of 115.392 t C/ha) with an annual carbon sequestration rate of 0.10 t C/ha from 1982–2015. The results suggest that NDVI variation is more sensitive to temperature than precipitation and it is valuable to measure carbon dynamics in Nepal.
General Practice Care for Patients with Rare Diseases in Belgium. A Cross-Sectional Survey
Authors: Boffin Nicole , Swinnen Elfriede , Wens Johan , Urbina Montse , Van der Heyden Johan , Van Casteren Viviane
Published: June 2018

There are almost no studies about rare diseases in general practice. This study examined care characteristics of active rare disease patients in the Belgian Network of Sentinel General Practices (SGP) and the importance of rare diseases in general practice by its caseload, general practitioner (GP)–patient encounter frequency and nationwide prevalence. The SGP reported data about: (i) the number of active rare disease patients in 2015; and (ii) characteristics of one to three most recently seen patients. Rare diseases were matched against Orphanet (www.orpha.net). GP encounter frequency and patients’ age were compared to the total general practice population. Details from 121 active patients (median age: 44, interquartile range (IQR) 24–60) showed that for 36.9% the GP had been the first caregiver for the rare disease and for 35.8% the GP established a diagnostic referral. GPs rated their knowledge about their patients’ disease as moderate and used Orphanet for 14.9% of patients. Any active rare disease patients (median: 1, IQR 0–2) were reported by 66 of 111 SGP. Compared to the total general practice population, the mean GP encounter frequency was higher (7.3; 95% confidence intervals (CI) 6.1–8.5 versus 5.4; 95% CI 5.4–5.4). The prevalence of rare diseases in the Belgian general practice population was estimated at 12.0 (95% CI 10.3–13.9) per 10,000. This study acknowledges the important role of GPs in rare disease care. Knowledge and use of Orphanet by GPs could be improved.
Wealth Effects on Household Final Consumption: Stock and Housing Market Channels
Authors: Coskun Yener , Sencer Atasoy Burak , Morri Giacomo , Alp Esra
Published: June 2018

The study primarily explores the linkage between wealth effects, arising from stock and housing market channels, and household final consumption for 11 advanced countries over the period from 1970 Q1 to 2015 Q4. As a modelling strategy, we employ regression analysis through the common correlated effects mean group (CCEMG) estimator, as well as Durbin–Hausman cointegration and Dumitrescu and Hurlin (2012) causality tests. The study provides various pieces of evidence through whole-panel and country-level analyses. In this respect, we find that consumption is mostly explained by income and housing wealth is positively and significantly correlated with consumption. As counter-intuitive evidence, we detect a negative linkage between consumption and stock wealth. The evidence also suggests a long-run cointegration relationship among consumption, income, interest rates, housing wealth, and stock wealth. Moreover, we find bidirectional causality between consumption and income, stock wealth, housing wealth, and interest rates. Overall, the evidence implies that housing wealth, rather than stock wealth, is the primary source of consumption growth in advanced countries.
Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content
Authors: Aoyagi Toshihiro , Kobayashi Masaya , Kozaki Akiko
Published: June 2018

Increasing the yield of plant oil is an important objective to meet the demand for sustainable resources and energy. Some attempts to enhance the expression of genes involved in oil synthesis in seeds have succeeded in increasing oil content. In many cases, the promoters of seed-storage protein genes have been used as seed-specific promoters. However, conventional promoters are developmentally regulated and their expression periods are limited. We constructed a chimeric promoter that starts to express in the early stage of seed development, and high-level expression is retained until the later stage by connecting the promoters of the biotin carboxyl carrier protein 2 (BCCP2) gene encoding the BCCP2 subunit of acetyl-CoA carboxylase and the fatty acid elongase 1 (FAE1) gene from Arabidopsis. The constructed promoter was ligated upstream of the TAG1 gene encoding diacylglycerol acyltransferase 1 and introduced into Arabidopsis. Seeds from transgenic plants carrying AtTAG1 under the control of the chimeric promoter showed increased oil content (up by 18–73%) compared with wild-type seeds. The novel expression profile of the chimeric promoter showed that this could be a promising strategy to manipulate the content of seed-storage oils and other compounds.
Methyl 3,4-Dihydroxybenzoate Enhances Resistance to Oxidative Stressors and Lifespan in C. elegans Partially via daf-2/daf-16
Authors: Mi Xiang-Nan , Wang Li-Fang , Hu Yang , Pan Jun-Ping , Xin Yi-Rong , Wang Jia-Hui , Geng Hai-Ju , Hu Song-Hui , Gao Qin , Luo Huan-Min
Published: June 2018

Genetic studies have elucidated mechanisms that regulate aging; however, there has been little progress in identifying drugs that retard ageing. Caenorhabditis elegans is among the classical model organisms in ageing research. Methyl 3,4-dihydroxybenzoate (MDHB) can prolong the life-span of C. elegans, but the underlying molecular mechanisms are not yet fully understood. Here, we report that MDHB prolongs the life-span of C. elegans and delays age-associated declines of physiological processes. Besides, MDHB can lengthen the life-span of eat-2 (ad1113) mutations, revealing that MDHB does not work via caloric restriction (CR). Surprisingly, the life-span–extending activity of MDHB is completely abolished in daf-2 (e1370) mutations, which suggests that daf-2 is crucial for a MDHB-induced pro-longevity effect in C. elegans. Moreover, MDHB enhances the nuclear localization of daf-16/FoxO, and then modulates the expressions of genes that positively correlate with defenses against stress and longevity in C. elegans. Therefore, our results indicate that MDHB at least partially acts as a modulator of the daf-2/daf-16 pathway to extend the lifespan of C. elegans, and MDHB might be a promising therapeutic agent for age-related diseases.
Role of Cryptochrome-1 and Cryptochrome-2 in Aldosterone-Producing Adenomas and Adrenocortical Cells
Authors: Tetti Martina , Castellano Isabella , Veneziano Francesca , Magnino Corrado , Veglio Franco , Mulatero Paolo , Monticone Silvia
Published: June 2018

Mice lacking the core-clock components, cryptochrome-1 (CRY1) and cryptochrome-2 (CRY2) display a phenotype of hyperaldosteronism, due to the upregulation of type VI 3β-hydroxyl-steroid dehydrogenase (Hsd3b6), the murine counterpart to the human type I 3β-hydroxyl-steroid dehydrogenase (HSD3B1) gene. In the present study, we evaluated the role of CRY1 and CRY2 genes, and their potential interplay with HSD3B isoforms in adrenal pathophysiology in man. Forty-six sporadic aldosterone-producing adenomas (APAs) and 20 paired adrenal samples were included, with the human adrenocortical cells HAC15 used as the in vitro model. In our cohort of sporadic APAs, CRY1 expression was 1.7-fold [0.75–2.26] higher (p = 0.016), while CRY2 showed a 20% lower expression [0.80, 0.52–1.08] (p = 0.04) in APAs when compared with the corresponding adjacent adrenal cortex. Type II 3β-hydroxyl-steroid dehydrogenase (HSD3B2) was 317-fold [200–573] more expressed than HSD3B1, and is the main HSD3B isoform in APAs. Both dehydrogenases were more expressed in APAs when compared with the adjacent cortex (5.7-fold and 3.5-fold, respectively, p < 0.001 and p = 0.001) and HSD3B1 was significantly more expressed in APAs composed mainly of zona glomerulosa-like cells. Treatment with angiotensin II (AngII) resulted in a significant upregulation of CRY1 (1.7 ± 0.25-fold, p < 0.001) at 6 h, and downregulation of CRY2 at 12 h (0.6 ± 0.1-fold, p < 0.001), through activation of the AngII type 1 receptor. Independent silencing of CRY1 and CRY2 genes in HAC15 cells resulted in a mild upregulation of HSD3B2 without affecting HSD3B1 expression. In conclusion, our results support the hypothesis that CRY1 and CRY2, being AngII-regulated genes, and showing a differential expression in APAs when compared with the adjacent adrenal cortex, might be involved in adrenal cell function, and in the regulation of aldosterone production.
Niemann-Pick Type C2 Protein Regulates Free Cholesterol Accumulation and Influences Hepatic Stellate Cell Proliferation and Mitochondrial Respiration Function
Authors: Wang Yuan-Hsi , Twu Yuh-Ching , Wang Chung-Kwe , Lin Fu-Zhen , Lee Chun-Ya , Liao Yi-Jen
Published: June 2018

Liver fibrosis is the first step toward the progression to cirrhosis, portal hypertension, and hepatocellular carcinoma. A high-cholesterol diet is associated with liver fibrosis via the accumulation of free cholesterol in hepatic stellate cells (HSCs). Niemann-Pick type C2 (NPC2) plays an important role in the regulation of intracellular free cholesterol homeostasis via direct binding with free cholesterol. Previously, we reported that NPC2 was downregulated in liver cirrhosis tissues. Loss of NPC2 enhanced the accumulation of free cholesterol in HSCs and made them more susceptible to transforming growth factor (TGF)-β1. In this study, we showed that knockdown of NPC2 resulted in marked increases in platelet-derived growth factor BB (PDGF-BB)-induced HSC proliferation through enhanced extracellular signal-regulated kinases (ERK), p38, c-Jun N-terminal kinases (JNK), and protein kinase B (AKT) phosphorylation. In contrast, NPC2 overexpression decreased PDGF-BB-induced cell proliferation by inhibiting p38, JNK, and AKT phosphorylation. Although NPC2 expression did not affect caspase-related apoptosis, the autophagy marker light chain 3β (LC3B) was decreased in NPC2 knockdown, and free cholesterol accumulated in the HSCs. The mitochondrial respiration functions (such as oxygen consumption rate, ATP production, and maximal respiratory capacity) were decreased in NPC2 knockdown, and free cholesterol accumulated in the HSCs, while NPC2-overexpressed cells remained normal. In addition, NPC2 expression did not affect the susceptibility of HSCs to lipopolysaccharides (LPS), and U18666A treatment induced free cholesterol accumulation, which enhanced LPS-induced Toll-like receptor 4 (TLR4), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 phosphorylation, interleukin (IL)-1 and IL-6 expression. Our study demonstrated that NPC2-mediated free cholesterol homeostasis controls HSC proliferation and mitochondrial function.
Impact of Reciprocity in Information Spreading Using Epidemic Model Variants
Authors: Narang Rishabh , Sarin Simran , Singh Prajjwal , Goyal Rinkaj
Published: June 2018

The use of online social networks has become a standard medium of social interactions and information spreading. Due to the significant amount of data available online, social network analysis has become apropos to the researchers of diverse domains to study and analyse innovative patterns, friendships, and relationships. Message dissemination through these networks is a complex and dynamic process. Moreover, the presence of reciprocal links intensify the whole process of propagation and expand the chances of reaching to the target node. We therefore empirically investigated the relative importance of reciprocal relationships in the directed social networks affecting information spreading. Since the dynamics of the information diffusion has considerable qualitative similarities with the spread of infections, we analysed six different variants of the Susceptible–Infected (SI) epidemic spreading model to evaluate the effect of reciprocity. By analysing three different directed networks on different network metrics using these variants, we establish the dominance of reciprocal links as compared to the non-reciprocal links. This study also contributes towards a closer examination of the subtleties responsible for maintaining the network connectivity.
Ni(NH3)2(NO3)2—A 3-D Network through Bridging Nitrate Units Isolated from the Thermal Decomposition of Nickel Hexammine Dinitrate
Authors: Breternitz Joachim , Godula-Jopek Agata , Gregory Duncan H.
Published: June 2018

Nickel nitrate diammine, Ni(NH3)2(NO3)2, can be synthesised from the thermal decomposition of nickel nitrate hexammine, Ni[(NH3)6](NO3)2. The hexammine decomposes in two distinct major stages; the first releases 4 equivalents of ammonia while the second involves the release of NOx, N2, and H2O to yield NiO. The intermediate diammine compound can be isolated following the first deammoniation step or synthesised as a single phase from the hexammine under vacuum. Powder X-ray diffraction (PXD) experiments have allowed the structure of Ni(NH3)2(NO3)2 to be solved for the first time. The compound crystallises in orthorhombic space group Pca21 (a = 11.0628 (5) Å, b = 6.0454 (3) Å, c = 9.3526 (4) Å; Z = 4) and contains 11 non-hydrogen atoms in the asymmetric unit. Fourier transform infrared (FTIR) spectroscopy demonstrates that the bonding in the ammine is consistent with the structure determined by PXD.
E-Textile Embroidered Metamaterial Transmission Line for Signal Propagation Control
Authors: Moradi Bahareh , Fernández-García Raul , Gil Ignacio
Published: June 2018

In this paper, the utilization of common fabrics for the manufacturing of e-textile metamaterial transmission lines is investigated. In order to filter and control the signal propagation in the ultra-high frequency (UHF) range along the e-textile, a conventional metamaterial transmission line was compared with embroidered metamaterial particles. The proposed design was based on a transmission line loaded with one or several split-ring resonators (SRR) on a felt substrate. To explore the relations between physical parameters and filter performance characteristics, theoretical models based on transmission matrices’ description of the filter constituent components were proposed. Excellent agreement between theoretical prediction, electromagnetic simulations, and measurement were found. Experimental results showed stop-band levels higher than −30 dB for compact embroidered metamaterial e-textiles. The validated results confirmed embroidery as a useful technique to obtain customized electromagnetic properties, such as filtering, on wearable applications.
Wide-Band Circularly Polarized ReflectarrayUsing Graphene-Based Pancharatnam-Berry Phase Unit-Cells for Terahertz Communication
Authors: Deng Li , Zhang Yuanyuan , Zhu Jianfeng , Zhang Chen
Published: June 2018

A wide-band and high gain circularly polarized (CP) graphene-based reflectarray operating in the THz regime is proposed and theoretically investigated in this paper. The proposed reflectarray consists of a THz CP source and several graphene-based unit-cells. Taking advantages of the Pancharatnam Berry (PB) phase principle, the graphene-based unit-cell is capable of realizing a tunable phase range of 360° in a wide-band (1.4–1.7 THz) by unit-cell rotating, overcoming the restriction of intrinsic narrow-band resonance in graphene. Therefore, this graphene-based unit-cell exhibits superior bandwidth and phase tunability to its previous counterparts. To demonstrate this, a wide-band (1.4–1.7 THz) focusing metasurface based on the proposed unit-cell that exhibits excellent focusing effect was designed. Then, according to the reversibility of the optical path, a CP reflectarray was realized by placing a wide-band CP THz source at the focal point of the metasurface. Numerical simulation demonstrates that this reflectarray can achieve a stable high gain up to 15 dBic and an axial ratio around 2.1 dB over the 1.4–1.7 THz band. The good radiation performance of the proposed CP reflectarray, as demonstrated, underlines its suitability for the THz communication applications. Moreover, the design principle of this graphene-based reflectarray with a full 360° phase range tunable unit-cells provides a new pathway to design high-performance CP reflectarray in the THz regime.
Efficacy of Four Solanum spp. Extracts in an Animal Model of Cutaneous Leishmaniasis
Authors: Cos Paul , Janssens Jo , Piñón Abel , Cuesta-Rubio Osmany , Yglesias-Rivera Arianna , Díaz-García Alexis , Vilegas Wagner , Monzote Lianet
Published: June 2018

Background: Leishmaniasis is a complex protozoa disease caused by Leishmania genus (Trypanosomatidae family). Currently, there have been renewed interests worldwide in plants as pharmaceutical agents. In this study, the in vivo efficacy of Solanum spp. is assessed in an L. amazonensis BALB/c mice model for experimental cutaneous leishmaniasis. Methods: Animals were infected with 5 × 106 metacyclic promastigotes and 30-day post-infection, a treatment with 30 mg/kg of Solanum extracts or Glucantime® (GTM) was applied intralesionally every four days to complete 5 doses. Results: Neither death nor loss of weight higher than 10% was observed. All the tested extracts were able to control the infection, compared with the infected and untreated group. Solanum havanense Jacq. extract showed the highest efficacy and was superior (p < 0.05) to GTM. Solanum myriacanthum Dunal., S. nudum Dunal. and S. seaforthianum Andr. extracts demonstrated a similar effect (p > 0.05) to GTM. An increase of IFN-γ (p < 0.05) was displayed only by animals treated with S. nudum compared to the group treated with a vehicle, while no differences (p > 0.05) were observed for IL-12. Conclusions: In vivo effects of Solanum extracts were demonstrated, suggesting that this genus could be further explored as a new antileishmanial alternative.
Comparative Study of the Uniaxial Cyclic Behaviour of Carbide-Bearing and Carbide-Free Bainitic Steels
Authors: Branco Ricardo , Berto Filippo , Zhang Fucheng , Long Xiaoyan , Costa José Domingos
Published: June 2018

Bainitic steels play an important role in the modern automotive and rail industries because of their balanced properties. Understanding the relationship between the bainitic microstructure features and the fatigue performance is a fundamental ingredient in developing safer and durable products. However, so far this relationship is not sufficiently clear. Therefore, there is the need to strengthen the knowledge within this field. The present paper aims at comparing the uniaxial cyclic behaviour of carbide-bearing and carbide-free bainitic steels. To meet this goal, fully-reversed strain-controlled tests at various strain amplitudes were performed. After the final failure, fracture surfaces were observed by transmission electron microscopy to relate the bainitic morphology to the fatigue performance. The main findings of this work show that the carbide-free lower bainite has superior fatigue performance compared to the carbide-bearing lower bainite. This is explained by the presence of stable carbides and thick bainite ferrite plates.
An Overview of Metal Matrix Nanocomposites Reinforced with Graphene Nanoplatelets; Mechanical, Electrical and Thermophysical Properties
Authors: Saboori Abdollah , Dadkhah Mehran , Fino Paolo , Pavese Matteo
Published: June 2018

Two-dimensional graphene nanoplatelets with unique electrical, mechanical and thermophysical characteristics are considered as an interesting reinforcement to develop new lightweight, high-strength, and high-performance metal matrix nanocomposites. On the other hand, by the rapid progress of technology in recent years, development of advanced materials like new metal matrix nanocomposites for structural engineering and functional device applications is a priority for various industries. This article provides an overview of research efforts with an emphasis on the fabrication and characterization of different metal matrix nanocomposites reinforced by graphene nanoplatelets (GNPs). Particular attention is devoted to find the role of GNPs on the final electrical and thermal conductivity, the coefficient of thermal expansion, and mechanical responses of aluminum, magnesium and copper matrix nanocomposites. In sum, this review pays specific attention to the structure-property relationship of these novel nanocomposites.
Mechanical Properties and Corrosion Behavior of WZ73 Mg Alloy/SiCp Composite Fabricated by Stir Casting Method
Authors: Chiu Chun , Liu Hsu-Chieh
Published: June 2018

Low strength, which limits the industrial applications of Mg alloys, can be improved by forming Mg-based metal matrix composites (MMC) reinforced with ceramic particles. In this study, a Mg-based MMC was synthesized by introducing SiC particles into a WZ73 Mg alloy using the stir casting method. The effects of the SiC particles on the mechanical properties and corrosion behavior of WZ73 alloys were studied. The results showed that an addition of 1.5 vol % of SiC enhanced the strength of a WZ73 alloy but reduced the corrosion resistance. A further increase of SiC to 2.5 vol % had no effects on strength and corrosion behavior, which was attributed to the agglomeration of SiC particles. A microstructural analysis indicated that the addition of SiC did not alter the morphology and distribution of the secondary phase in the WZ73 alloy. Thus, the improved strength was attributed to the reinforcement of SiC and the refinement of the Mg grain, while the degraded corrosion resistance was the result of the grain refinement of Mg and the presence of the Mg/SiC interface in the vicinity of the secondary phase, which breaks the continuity of the Mg matrix and results in a higher corrosion rate.
Roselle Anthocyanins: Antioxidant Properties and Stability to Heat and pH
Authors: Wu Hai-Yao , Yang Kai-Min , Chiang Po-Yuan
Published: June 2018

Roselle is rich in anthocyanins and is traditionally used to prepare a bright red beverage by decoction. However, heat treatment and different pH environments are often encountered during food processing, and these factors are often detrimental to anthocyanins. Therefore, it is very important to understand the influence of pH and heat treatment on anthocyanins for the application of roselle. This study determined the antioxidant properties of roselle extract, explored changes in the color and anthocyanin content in different pH environments, and evaluated the thermal stability of roselle anthocyanins using kinetic equations. The results showed that the roselle extract is rich in anthocyanins and has good antioxidant capacity (DPPH IC50 = 4.06 mg/mL, ABTS IC50 = 3.7 mg/mL). The anthocyanins themselves exhibited a certain degree of heat resistance and good color stability in an acidic environment. In contrast, they degraded very quickly and exhibited significant changes in color in a low-acid environment. The activation energy (Ea) ranges of the anthocyanins in the acidic and low-acid environments were quite different at 55.8–95.7 and 31.4–74.9 kJ/mol, respectively. Thus, it can be concluded that roselle anthocyanins are susceptible to heat treatment in a low-acid environment, affecting their quality and appearance; however, they can serve as a good source of functional ingredients and color in an acidic environment.
Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation
Authors: Choi Hyun-Ji , Lee Han-Byul , Jung Sunyoung , Park Hyun-Kyu , Jo Woori , Cho Sung-Min , Kim Woo-Jin , Son Woo-Chan
Published: June 2018

The Sleeping Beauty (SB) transposon system is non-viral and uses insertional mutagenesis, resulting in the permanent expression of transferred genes. Although the SB transposon is a useful method for establishing a mouse tumor model, there has been difficulty in using this method to generate tumors in the prostate. In the present study, electroporation was used to enhance the transfection efficiency of the SB transposon. To generate tumors, three constructs (a c-Myc expression cassette, a HRAS (HRas proto-oncogene, GTPase) expression cassette and a shRNA against p53) contained within the SB transposon plasmids were directly injected into the prostate. Electroporation was conducted on the injection site after the injection of the DNA plasmid. Following the tumorigenesis, the tumors were monitored by animal PET imaging and identified by gross observation. After this, the tumors were characterized by using histological and immunohistochemical techniques. The expression of the targeted genes was analyzed by Real-Time qRT-PCR. All mice subjected to the injection were found to have prostate tumors, which was supported by PSA immunohistochemistry. To our knowledge, this is the first demonstration of tumor induction in the mouse prostate using the electroporation-enhanced SB transposon system in combination with c-Myc, HRAS and p53. This model serves as a valuable resource for the future development of SB-induced mouse models of cancer.
Insights into Metal Oxide and Zero-Valent Metal Nanocrystal Formation on Multiwalled Carbon Nanotube Surfaces during Sol-Gel Process
Authors: Das Dipesh , Sabaraya Indu V. , Sabo-Attwood Tara , Saleh Navid B.
Published: June 2018

Carbon nanotubes are hybridized with metal crystals to impart multifunctionality into the nanohybrids (NHs). Simple but effective synthesis techniques are desired to form both zero-valent and oxides of different metal species on carbon nanotube surfaces. Sol-gel technique brings in significant advantages and is a viable technique for such synthesis. This study probes the efficacy of sol-gel process and aims to identify underlying mechanisms of crystal formation. Standard electron potential (SEP) is used as a guiding parameter to choose the metal species; i.e., highly negative SEP (e.g., Zn) with oxide crystal tendency, highly positive SEP (e.g., Ag) with zero-valent crystal-tendency, and intermediate range SEP (e.g., Cu) to probe the oxidation tendency in crystal formation are chosen. Transmission electron microscopy and X-ray diffraction are used to evaluate the synthesized NHs. Results indicate that SEP can be a reliable guide for the resulting crystalline phase of a certain metal species, particularly when the magnitude of this parameter is relatively high. However, for intermediate range SEP-metals, mix phase crystals can be expected. For example, Cu will form Cu2O and zero-valent Cu crystals, unless the synthesis is performed in a reducing environment.
Iron Promotes Intestinal Development in Neonatal Piglets
Authors: Pu Yutian , Li Shuhui , Xiong Haitao , Zhang Xiaofeng , Wang Yizhen , Du Huahua
Published: June 2018

Early nutrition is key to promoting gut growth and education of the immune system. Although iron deficiency anemia has long been recognized as a serious iron disorder, the effects of iron supplementation on gut development are less clear. Therefore, using suckling piglets as the model for iron deficiency, we assessed the impacts of iron supplementation on hematological status, gut development, and immunity improvement. Piglets were parenterally supplied with iron dextran (FeDex, 60 mg Fe/kg) by intramuscular administration on the third day after birth and slaughtered at the age of two days, five days, 10 days, and 20 days. It was expected that iron supplementation with FeDex improved the iron status with higher levels of serum iron, ferritin, transferrin, and iron loading in the liver by regulating the interaction of hepcidin and ferroportin (FPN). FeDex supplementation increased villus length and crypt depth, attenuated the pathological status of the duodenum, and was beneficial to intestinal mucosa. FeDex also influenced the intestinal immune development by stimulating the cytokines’ production of the intestine and enhancing the phagocytotic capacity of monocytes. Overall, the present study suggested that iron supplementation helped promote the development of the intestine by improving its morphology, which maintains its mucosal integrity and enhances the expression of immuno-associated factors.
Structural and Thermoanalytical Characterization of 3D Porous PDMS Foam Materials: The Effect of Impurities Derived from a Sugar Templating Process
Authors: González-Rivera José , Iglio Rossella , Barillaro Giuseppe , Duce Celia , Tinè Maria Rosaria
Published: June 2018

Polydimethylsiloxane (PDMS) polymers are extensively used in a wide range of research and industrial fields, due to their highly versatile chemical, physical, and biological properties. Besides the different two-dimensional PDMS formulations available, three-dimensional PDMS foams have attracted increased attention. However, as-prepared PDMS foams contain residual unreacted low molecular weight species that need to be removed in order to obtain a standard and chemically stable material for use as a scaffold for different decorating agents. We propose a cleaning procedure for PDMS foams obtained using a sugar templating process, based on the use of two different solvents (hexane and ethanol) as cleaning agents. Thermogravimetry coupled with Fourier Transform Infrared Spectroscopy (TG-FTIR) for the analysis of the evolved gasses was used to characterize the thermal stability and decomposition pathway of the PDMS foams, before and after the cleaning procedure. The results were compared with those obtained on non-porous PDMS bulk as a reference. Micro-CT microtomography and scanning electron microscopy (SEM) analyses were employed to study the morphology of the PDMS foam. The thermogravimetric analysis (TGA) revealed a different thermal behaviour and crosslinking pathway between bulk PDMS and porous PDMS foam, which was also influenced by the washing process. This information was not apparent from spectroscopic or morphological studies and it would be very useful for planning the use of such complex and very reactive systems.
Acetyl Groups in Typha capensis: Fate of Acetates during Organosolv and Ionosolv Pulping
Authors: Audu Idi Guga , Brosse Nicolas , Winter Heiko , Hoffmann Anton , Bremer Martina , Fischer Steffen , Laborie Marie-Pierre
Published: June 2018

During biomass fractionation, any native acetylation of lignin and heteropolysaccharide may affect the process and the resulting lignin structure. In this study, Typha capensis (TC) and its lignin isolated by milling (MWL), ionosolv (ILL) and organosolv (EOL) methods were investigated for acetyl group content using FT-Raman, 1H NMR, 2D-NMR, back-titration, and Zemplén transesterification analytical methods. The study revealed that TC is a highly acetylated grass; extractive free TC (TCextr) and TC MWL exhibited similar values of acetyl content: 6 wt % and 8 wt % by Zemplén transesterification, respectively, and 11 wt % by back-titration. In contrast, lignin extracted from organosolv and [EMIm][OAc] pulping lost 80% of the original acetyl groups. With a high acetyl content in the natural state, TC could be an interesting raw material in biorefinery in which acetic acid could become an important by-product.
Model Studies on Load-Settlement Characteristics of Coarse-Grained Soil Treated with Geofiber and Cement
Authors: Li Yan , Su Lei , Ling Xianzhang , Wang Jiahui , Yang Yingzi
Published: June 2018

This study aims to verify the effectiveness of fiber reinforcing with and without cement on settlement controlling of subgrade models, and to investigate the effect of fiber reinforcement on the load-settlement behavior of subgrade models. To this end, laboratory subgrade model tests were conducted under different static vertical loads. Three subgrade models composed of different fillers were constructed in a rigid concrete tank, and the internal earth pressures and settlements at different depths were measured through an earth pressure cell and settlement plate. Results show that the fiber-reinforced model keeps a slight difference to the unreinforced model in terms of earth pressure distribution under lower applied surface pressure. However, the earth pressure at various locations under each surface pressure was obviously lower than that of the other two models due to the combined effect of fiber and cement. In addition, for the unreinforced subgrade model, the 60 cm settlement domain was restricted within 40 cm depth through fiber-cement and fiber reinforcing, and the total settlement under 100 kPa was decreased by 48.5% and 30.8%, respectively. Moreover, reinforced models present with different settlement deformation features. The inflection points, after which the rate of settlement decreased with increasing applied surface pressure, were observed in the pressure-settlement curves. Under 200 kPa, the fiber-cement and fiber reinforcement decreased the total settlement of the unreinforced model by 61.4% and 34.7%, respectively. The greater applied surface pressure, the more efficient was fiber-cement reinforcing in settlement controlling.
Ten Years of TerraSAR-X Operations
Authors: Buckreuss Stefan , Schättler Birgit , Fritz Thomas , Mittermayer Josef , Kahle Ralph , Maurer Edith , Böer Johannes , Bachmann Markus , Mrowka Falk , Schwarz Egbert , Breit Helko , Steinbrecher Ulrich
Published: June 2018

The satellite of the TerraSAR-X mission, called TSX, was launched on 15 June 2007 and its identically constructed twin satellite TDX, which is required by the mission TanDEM-X, launched on 21 June 2010. Together they supply high-quality radar data in order to serve two mission goals: Scientific observation of Earth and the provisioning of remote sensing data for the commercial market (TerraSAR-X mission) and the generation of a global digital elevation model (DEM) of Earth’s surface (TanDEM-X mission). On the occasion of the 10th anniversary of the mission, the focus will be on the development of the TerraSAR-X system during this period, including the extension of the ground segment, the evolution of the product portfolio, dedicated mission campaigns, radar experiments, refinement of the satellite operations and orbit control, and the results of the performance monitoring. Despite numerous interventions in the overall system, we managed to incorporate new scientific and commercial requirements and to improve and enhance the overall system in order to fulfill the increasing demand for Earth observation data without noticeable interruptions to ongoing operations.
Mean Composite Fire Severity Metrics Computed with Google Earth Engine Offer Improved Accuracy and Expanded Mapping Potential
Authors: Parks Sean A. , Holsinger Lisa M. , Voss Morgan A. , Loehman Rachel A. , Robinson Nathaniel P.
Published: June 2018

Landsat-based fire severity datasets are an invaluable resource for monitoring and research purposes. These gridded fire severity datasets are generally produced with pre- and post-fire imagery to estimate the degree of fire-induced ecological change. Here, we introduce methods to produce three Landsat-based fire severity metrics using the Google Earth Engine (GEE) platform: The delta normalized burn ratio (dNBR), the relativized delta normalized burn ratio (RdNBR), and the relativized burn ratio (RBR). Our methods do not rely on time-consuming a priori scene selection but instead use a mean compositing approach in which all valid pixels (e.g., cloud-free) over a pre-specified date range (pre- and post-fire) are stacked and the mean value for each pixel over each stack is used to produce the resulting fire severity datasets. This approach demonstrates that fire severity datasets can be produced with relative ease and speed compared to the standard approach in which one pre-fire and one post-fire scene are judiciously identified and used to produce fire severity datasets. We also validate the GEE-derived fire severity metrics using field-based fire severity plots for 18 fires in the western United States. These validations are compared to Landsat-based fire severity datasets produced using only one pre- and post-fire scene, which has been the standard approach in producing such datasets since their inception. Results indicate that the GEE-derived fire severity datasets generally show improved validation statistics compared to parallel versions in which only one pre-fire and one post-fire scene are used, though some of the improvements in some validations are more or less negligible. We provide code and a sample geospatial fire history layer to produce dNBR, RdNBR, and RBR for the 18 fires we evaluated. Although our approach requires that a geospatial fire history layer (i.e., fire perimeters) be produced independently and prior to applying our methods, we suggest that our GEE methodology can reasonably be implemented on hundreds to thousands of fires, thereby increasing opportunities for fire severity monitoring and research across the globe.
Functionalized Fiber End Superstructure Fiber Bragg Grating Refractive Index Sensor for Heavy Metal Ion Detection
Authors: Tan Rex Xiao , Yap Stephanie Hui Kit , Tan Yung Chuen , Tjin Swee Chuan , Ibsen Morten , Yong Ken Tye , Lai Wenn Jing
Published: June 2018

We present a novel superstructure fiber Bragg grating fiber end sensor capable of detecting variations in refractive index (RI) of liquids and potentially that of gases, and demonstrated an application in the detection of heavy metal ions in water. The sensor is capable of sensing RI variations in the range of 1.333 to 1.470 with good sensitivity of up to 230 dB/RIU achieved for the RI range of 1.370 to 1.390. The sensor is capable of simultaneously measuring variations in ambient temperature along with RI. A simple chemical coating was employed as a chelating agent for heavy metal ion detection at the fiber end to demonstrate an possible application of the sensor. The coated fiber sensor can conclusively detect the presence of heavy metal ions with concentrations upwards of 100 ppm. RI sensing capability of the sensor is neither affected by temperature nor strain and is both robust and easily reproducible.