bluuebunny/crossref_metadata_2025 huggingface dataset:
Retrospective Analysis of the Clinical Efficacy of Early Goal-Directed Therapy Combined with Meticulous Nursing Intervention in Patients with Posttraumatic Sepsis
Authors: Yuan Zhe , Yang Shilu , Zhang Chunhua , Chen Ke , Wang Minhuan , Hao Shaoqian , Dong Shimin , Yang Yang
Published: December 2021

Objective. To explore the intervention effect of early goal-directed therapy (EGDT) combined with meticulous nursing on patients with posttraumatic sepsis. Methods. The data of 50 patients with posttraumatic sepsis undergoing EGDT in the emergency department of our hospital from January 2020 to December 2020 were retrospectively analyzed. According to different nursing methods, they were divided into control group (n = 25) with routine nursing measures and observation group (n = 25) with meticulous nursing measures. The application effect of the two nursing modes was scientifically evaluated. Results. No statistical differences in general data were found between the two groups (

P
>
0.05

). After 6 h of intervention, the circulatory function, oxygenation function, and renal function of both groups were better than those before intervention, and central venous pressure (CVP), mean arterial pressure (MAP), blood oxygen (PaO2), oxygenation index (PaO2/FiO2), central venous oxygen saturation (ScvO2), and urine volume in the observation group were notably higher than those in the control group (

P
<
0.05

). The heart rate (HR), serum creatinine (SCr), and blood lactic acid in the observation group were notably lower than those in the control group (

P
<
0.05

). The 28-day survival rate and quality of life after intervention in the observation group were notably higher than those in the control group, with obvious differences between the two groups (

P
<
0.05

). Conclusion. Meticulous nursing intervention for patients with posttraumatic sepsis undergoing EGDT can effectively improve the body’s functional indexes, which is superior to the routine nursing in controlling the patients’ condition, improving the survival rate and quality of life after intervention, and ensuring the clinical treatment effect. Therefore, it is worthy of promotion.
Prognostic Value of TRPM7 Expression and Factor XIIIa-Expressing Tumor-Associated Macrophages in Gastric Cancer
Authors: Calik Muhammet , Calik Ilknur , Artas Gokhan , Ozercan Ibrahim Hanifi
Published: December 2021

Purpose. TRPM7 is known to play a key role in tumor progression by regulating cellular proliferation, migration, and invasion in various cancer cell lines. However, there are no comprehensive clinical studies about the effect of TRPM7 expression on gastric cancer (GC) prognosis. In this study, it was aimed at investigating the effect of TRPM7 expression on prognosis in GC patients. Additionally, for the first time, it was investigated whether the density of Factor XIIIa-expressing tumor-associated macrophages (TAMs) in GC has an effect on the biological behaviour of the tumor. Methods. TRPM7 expression and Factor XIIIa-expressing TAM density were immunohistochemically evaluated in paraffin-embedded tumor tissues of 204 GC patients undergoing surgery at a single institution. Results. Tumor size was clearly higher in cases with high TRPM7 expression than those with low expression (

p
<
0.001

, Mann-Whitney

U

). TRPM7 overexpression was closely related to high depth of tumor invasion (

p
<
0.001

, ANOVA), increased lymph node metastasis (

p
<
0.001

, ANOVA), and high distant metastasis rate (

p
<
0.001

, Mann-Whitney

U

). These findings exposed that high TRPM7 expression is effective in the progression and aggressiveness of GC. In addition, while high CD8+ TIL density affects the prognosis positively, it was determined that high Factor XIIIa+ TAM density negatively affects the prognosis of patients with GC. Furthermore, multivariate analyses revealed TRPM7 overexpression was independently related with short overall (HR 9.64, 95% CI 5.74–16.19,

p
<
0.001

) and disease-free survival (HR 5.67, 95% CI 3.61-8.92,

p
<
0.001

) in GC patients. Conclusions. Our data suggest that high TRPM7 expression is closely related to progressive tumor behaviour in GC and independently negatively affects survival in patients. In addition, it was determined that a high density of Factor XIIIa+ TAMs negatively affects the prognosis of patients with GC.
Study of Crater in the Gobi Desert Induced by Ground Explosion of Large Amounts of TNT Explosive up to 10 Tons
Authors: Xu Rongzheng , Chen Li , Zheng Yuzhou , Li Zhan , Cao Mingjin , Fang Qin
Published: December 2021

Explosion craters on the ground surface induced by contact or near‐field explosions have important implications, which can be used to assess blast consequences, guide the design of the explosion, or develop a protective strategy. In this study, to understand the crater characteristics induced by the contact explosion of large weight explosives, four field contact explosion tests were conducted on the surface of the Gobi Desert with large TNT charge weights of 1 ton, 3 tons, and 10 tons (test conducted twice). Cratering on the ground surface generated by large amounts of explosives was measured and evaluated, including the shape, depth, and diameter. A fine‐mesh numerical model was developed and validated on the AUTODYN software platform, and a detailed parametric study was performed on the resulting craters. The effects of sand and gravel density, initiation method, shear modulus, and failure criteria were analyzed and discussed. An energy conversion coefficient was determined, and the corresponding theoretical equations were derived to predict the dimensions of the craters resulting from the large weight contact explosion. The calculated cratering characteristics were consistent with previous data and hence can be used in future engineering applications.
The Effectiveness and Safety of Probiotic Supplements for Psoriasis: A Systematic Review and Meta-Analysis of Randomized Controlled Trials and Preclinical Trials
Authors: Zeng Liuting , Yu Ganpeng , Wu Yang , Hao Wensa , Chen Hua
Published: December 2021

Background. Patients with psoriasis need long-term medication to control their condition. Recent studies suggest that changing the intestinal flora may be a potential treatment. Methods. The databases were utilized to search the randomized controlled trials (RCTs) and preclinical trials about probiotic supplement in the treatment of psoriasis. The retrieval time is from the establishment of these databases to December 2020. RevMan5.3 was used for the risk assessment of bias and meta-analysis. This systematic review was registered in PROSPERO (CRD42021232756). Results. A total of 3 RCTs involving 164 participants were included. Two RCTs showed that probiotics can improve PASI and thereby improve the condition. For inflammation-related indicators, only one RCT showed that probiotics can improve the levels of CRP and TNF-α but have no obvious improvement effect on IL6. One RCT demonstrated the total effective rate of probiotics in the treatment of psoriasis. For adverse events, one RCT showed that the incidence of adverse events of probiotic treatment was low. Preclinical studies showed that continuous intervention with oral probiotics can significantly improve the progression of psoriasis and reduce the expression of inflammatory factors. The meta-analysis showed that the PASI between two groups was of no statistical significance (SMD 1.83 [-0.41, 4.07],

P
=
0.11

). Meanwhile, probiotics may improve skin thickness (SMD -5.87 [-11.34, -0.41],

P
=
0.04

) in animal model. Conclusion. Prebiotics may have a positive effect on alleviating the clinical symptoms of psoriasis, but a large sample of RCTs is still needed to support its therapeutic effect in psoriasis.
Application of Artificial Intelligence and Wireless Networks to Music Teaching
Authors: Guan Yue , Ren Fangfang
Published: December 2021

The study is aimed at improving student’s learning ability and constructing an intelligent, all‐around, and three‐dimensional innovative classroom by using intelligent technology. Middle school C is taken as the research object. First, the current situation of music teaching in middle school C is analyzed, and an intelligent music teaching mode suitable for middle school C is designed. Second, the intelligent music teaching mode is applied in the actual teaching process, and students’ feelings for intelligent music classrooms are explored by a questionnaire survey. Finally, the teaching effect is analyzed. The results show that 96.7% of the students hold a positive attitude towards the new teaching mode, indicating that contemporary students have relatively high adaptation to and recognition with the development of artificial intelligence (AI). 84% of the students are not particularly skilled in the operation of the intelligent network platform, and 9.3% of the students believe that the operation of the platform is relatively difficult. Therefore, teachers should teach students how to use tablets. 95% of the students hold that teachers can quickly find teaching resources related to the course content on the tablet and introduce them to students in the teaching process, indicating that teachers are familiar with the usage of new teaching equipment. On the whole, students have a good experience of the new teaching mode. This new teaching mode based on AI is a response to the new course concept proposed in China.
Study on the Mechanism of Reducing Hepatotoxicity of Water-Grinding Realgar by Metabolomics, Morphology, and Chemical Analysis
Authors: Han Ting , Zhang Hui , Xu Wenjuan , Li Chunshuai , Wang Min , Bai Yuying , Yang Linlin , Zhang Shuyan , Jia Zhe , Xu Xinfang , Zhao Chongjun , Wei Feng , Li Xiangri
Published: December 2021

Background. Realgar was usually selected as a substitute for arsenic trioxide to treat acute promyelocytic leukemia due to its higher effect without high cardiotoxicity. In traditional Chinese medicine (TCM), realgar is usually processed by the water-grinding method clinically, but the mechanism of realgar processing detoxification is still unclear. However, it is necessary to take safety and efficacy into account while evaluating a drug. Methods. Sixty male Wistar rats were divided into control group, realgar products-treated groups, and corresponding subgroups. Biochemistry analysis and histopathological examination were performed in the study, and plasma samples were collected from all the rats for metabolomics analysis. Results. No significant toxicity was observed in rats treated with 0.64 g/kg/day grinding realgar (G-r) and water-grinding realgar (WG-r). When the dose increased to 1.92 g/kg/day, the liver weight coefficients of the rats treated with G-r (HG-r: 3.65 ± 0.26%) and WG-r (HWG-r: 3.67 ± 0.14%) increased significantly and severe hepatic injury occurred in comparison to the control group (Group C: 3.00 ± 0.21%). After one week's withdrawal, the liver injury caused by the high dose of WG-r significantly recovered, while the liver damage caused by G-r was more difficult to recover. In metabolomics analysis, 14 metabolites were identified as the potential biomarkers in realgar-treated rats. These metabolites indicated that there were perturbations of the primary bile acid biosynthesis, arachidonic acid metabolism, linoleic acid metabolism, and glycerophospholipid metabolism in the realgar-treated groups. Conclusions. These results illustrate that, as a TCM processing method, water grinding had the effect of reducing toxicity, and the metabolomics method may be a valuable tool for studying the toxicity induced by TCM and the mechanism of TCM processing.
Preparation and Characterization of Organic/Inorganic Composite UV Filter Microcapsules by Sol‐Gel Method
Authors: Wu Pey-Shiuan , Lin Chia-Hui , Kuo Yi-Ching , Lin Chih-Chien
Published: December 2021

Octyl methoxycinnamate and butyl methoxydibenzoylmethane are organic UV filters with poor photostability and will become photoallergy or phototoxic substance when exposed to ultraviolet radiation. The organic UV filters coated by microcapsules can reduce the photodegradation and avoid direct contact with the skin. Through microencapsulation, the application of UV filters in cosmetics becomes more effective and safer. This study first used the sol‐gel method to create organic/inorganic composite UV filter microcapsules. We used sodium alginate as a shell material of the microcapsule to encapsulate UV filters. CaCO3 and tetraethyl orthosilicate (TEOS) were used as cross‐linking agents, and sorbitan monooleate (Span 80) and polyoxyethylenesorbitan monooleate (Tween 80) were used as emulsifiers in the interfacial polymerization method for preparation. The results indicated that the microcapsules with 3 g of CaCO3 cross‐linking agents had a similar particle size and better entrapment efficiency. The average sizes were 61.0 ± 4.9 μm and 48.6 ± 4.7 μm, and entrapment efficiencies were 75.3 ± 1.9% and 74.8 ± 1.7% for octyl methoxycinnamate and butyl methoxydibenzoylmethane, respectively. Utilizing sodium alginate as a cross‐linking agent is better than TEOS due to the higher calcium content. In vitro transdermal delivery analysis showed that the release rate became steady.
The Role of HLA in the Association between IgA Deficiency and Celiac Disease
Authors: Poddighe Dimitri , Capittini Cristina
Published: December 2021

Selective IgA deficiency (SIgAD) is the most frequent primary immune defect. Since SIgAD is not characterized by relevant infectious issues in most cases, it is often diagnosed during the diagnostic work up of several and different autoimmune disorders, which are associated with this primary immune defect. The genetic background of SIgAD is complex and three HLA haplotypes resulted to be more frequently associated with it; in detail, two of them include HLA-DQB

1

∗

02 allelic variants, which are essential predisposing factors to develop Celiac Disease (CD). Here, we discuss the evidence regarding the role of HLA in the etiopathogenesis of SIgAD and its association with CD. Actually, the HLA region seems to play a modest role in the genetic predisposition to SIgAD and we may speculate that the association with the HLA-DQB

1

∗

02 alleles (or haplotypes including them) could derive from its link with CD. Indeed, SIgAD and some related immunological alterations are likely to predispose to several autoimmune diseases (with and despite different HLA backgrounds), including CD, which is relatively common and directly associated with the HLA-DQB

1

∗

02 allelic variants coding the DQ2 heterodimer. Further and specific studies are needed to make final conclusions in this regard.
Soil Erodibility Analysis and Mapping in Gilgel Gibe-I Catchment, Omo-Gibe River Basin, Ethiopia
Authors: Tesfaye Gizaw , Ameyu Tolesa
Published: December 2021

The soil erosion factor, erodibility, measures the susceptibility of soil particles to transport and detachment by erosive agents. Soil erosion and sedimentation models use soil properties and erodibility as the main input. However, in developing countries such as Ethiopia, data on soil erosion and soil-related properties are limited. For this reason, different researchers use different data sources that are adopted from a large scale and come with very different results. For this reason, the study was proposed to analyze and map the soil erodibility of the catchment area using primary data. 80 mixed soil samples were taken from the catchment with GPS coordinates and analyzed in the laboratory for soil texture class and soil organic matter. Accordingly, sandy clay loam is a dominant soil texture class covering 65% of the catchment area with 2.46% average soil organic matter, which is high in the mountainous part and lower in the lower valley of the catchment area. Most of the catchment area, which accounts for more than 78% of the area, was dominated by medium- or coarse-grained soil structure, and in the upper parts of the catchment area, 21% of the catchment area was covered with fine-grained soil structure. Similarly, 66% of the catchment area was covered with slow to moderate soil permeability, followed by slow soil permeability covering 21% of the area. Finally, the soil erodibility value of the Gilgel Gibe-I catchment was determined to be 0.046 ton h·MJ−1·mm−1 with a range of 0.032 to 0.063 ton·h·MJ−1·mm−1. In general, soils with slow permeability, high silt content, and medium- to fine-grained soil structures are the most erodible. They are conveniently separate; they tend to crust and form high drainage. Knowing this, the catchment has a moderate soil erodibility value. Thus, the study recommends evidence of land cover and the protection of arable land through suitable soil and water protection measures to improve soil permeability and soil structure.
Development and Validation of the Health Literacy Assessment Instrument for Patients with Chronic Pain
Authors: Chen Siqing , Zhang Xingwei , Cao Meijuan , Zhao Bingyu , Fang Jie
Published: December 2021

A suitable health literacy assessment instrument for patients with chronic pain (HLCP) in China with good instrument’s psychometric properties is required. A theoretical framework for the HLCP was developed by adopting the hierarchical model of health literacy proposed by Nutbeam. The reliability and validity of the HLCP were tested in a cross-sectional survey of 237 chronic pain patients from three pain clinics and wards of Grade-3A hospitals in Zhejiang Province, China. The discriminant degree method, correlation analysis method, factor analysis method (exploratory factor analysis), half reliability, and other methods were utilized to screen items for inclusion in the final version of HLCP, and the fitness of the model was subsequently evaluated by confirmatory factor analysis. Cronbach’s alpha value and test-retest with two-week intervals were used to test the internal consistency and retest reliability of the HLCP. In the exploratory factor analysis, three domains, functional health literacy (10 items), interactive health literacy (14 items), and critical health literacy (7 items), comprising 31 items in total, were finally loaded; the model was determined to explain 70.9% of the total variance. HLCP’s effective assessment of the health literacy level of patients with chronic pain and its acceptable reliability and validity were revealed through the results.
Global Meta-Analysis on the Association between Behcet Syndrome and Polymorphisms from the HLA Class I (A, B, and C) and Class II (DRB1, DQB1, and DPB1) Genes
Authors: Capittini Cristina , Rebuffi Chiara , Lenti Marco Vincenzo , Di Sabatino Antonio , Tinelli Carmine , Martinetti Miryam , De Silvestri Annalisa
Published: December 2021

Behcet syndrome (BS) is a multisystemic perivasculitis whose genetic susceptibility is linked to HLA region. We first meta-analysed all HLA class I and II genes involved in BS susceptibility in all ethnic groups worldwide. We identified 1141 articles and finally included 31 case-control studies after multiple rounds of selection. We analysed frequencies for 24 HLA-A alleles (3 alleles for HLA-A

∗

26 at four digits), 50 HLA-B alleles (11 alleles for HLA-B

∗

51 at four digits), 15 HLA-C alleles, 16 HLA-DRB1 alleles, 6 HLA-DQB1 alleles, and 15 HLA-DPB1 alleles. We meta-analysed only HLA allelic frequencies from at least three studies; therefore, we investigated 21 alleles out of 140. Going from 7.00 to 1.6 OR, we found 11 class I alleles conferring risk for BS:

B
∗
51
:
08

,

B
∗
51

,

B
∗
51
:
01

,

B
∗
51
:
02

,

DQB
1
∗
03

,

A
∗
26
:
01

,

Cw
∗
14

,

Cw
∗
15

,

Cw
∗
16

,

B
∗
15

, and

A
∗
26

. Overall, the studies included populations from Europe (Greece, Spain, Italy, Germany, and Ireland), Asia (Korea, China, China Han, and Thailand), Middle East (Israel, Saudi Arabia, and Iran), and Morocco (as no other North-African population was included). We collected a number of ethnical groups sufficient to conduct an ethnic-specific meta-analysis where Europeans showed 11.25 OR for B

∗

51:08 and Japan 3.50 OR for

A
∗
26
:
01

. A remarkable result was that the most frequent

HLA
−
B
∗
51

two-digit alleles associated with BS were different among populations:

HLA
−
B
∗
51
:
08

in Europe,

HLA
−
B
∗
51
:
01

in Turkey, and

HLA
−
B
∗
51
:
02

in Japan. Overall, we discussed our real-world results with other imputation studies.
Animation Art Design Online System Based on Mobile Edge Computing and User Perception
Authors: Liu Jianzhi
Published: December 2021

Based on mobile edge computing and user perception technology, this paper analyzes and discusses the respective advantages and disadvantages of the important optimization models and mobile models in the animation art design, as well as the wireless block data transmission mechanism and protocol. In order to solve the problem that user mobility cannot be sensed, a content‐centric mobile edge animation art design mechanism based on user mobility perception is proposed. This mechanism comprehensively calculates the centrality of users’ perception of nodes, the idle rate of animation design, and the staying time of users in a small area. The mobile edge network controller integrates the information of each edge user’s perception node, calculates the importance of each edge user’s perception node and prioritizes it, and selects the appropriate content animation to design the user perception node according to the ranking result. Finally, various simulation or platform test experiments were carried out for all the design schemes in this paper, and the experimental results were analyzed. The simulation experiment results show that compared with the traditional animation design mechanism, the animation art design system effectively reduces the average number of hops for users to obtain content by up to 15.9%, improves the hit rate of edge user perception node animation design by at least 13.7%, and reduces the traffic entering the core network by up to 32.1%. According to the comparison results, the various designs in this work can successfully use sensor data to preclassify migration tasks in the mobile edge network environment. Compared with the latest block data transmission protocol, it has a significant performance improvement, reducing the data distribution delay by 34.8%, thereby helping to improve the overall efficiency of mobile edge computing.
Too Good to Be True? Evaluation of Colonoscopy Sensitivity Assumptions Used in Policy Models
Authors: Rutter Carolyn M. , Nascimento de Lima Pedro , Lee Jeffrey K. , Ozik Jonathan
Published: December 2021

Background:

Models can help guide colorectal cancer screening policy. Although models are carefully calibrated and validated, there is less scrutiny of assumptions about test performance.

Methods:

We examined the validity of the CRC-SPIN model and colonoscopy sensitivity assumptions. Standard sensitivity assumptions, consistent with published decision analyses, assume sensitivity equal to 0.75 for diminutive adenomas (<6 mm), 0.85 for small adenomas (6–10 mm), 0.95 for large adenomas (≥10 mm), and 0.95 for preclinical cancer. We also selected adenoma sensitivity that resulted in more accurate predictions. Targets were drawn from the Wheat Bran Fiber study. We examined how well the model predicted outcomes measured over a three-year follow-up period, including the number of adenomas detected, the size of the largest adenoma detected, and incident colorectal cancer.

Results:

Using standard sensitivity assumptions, the model predicted adenoma prevalence that was too low (42.5% versus 48.9% observed, with 95% confidence interval 45.3%–50.7%) and detection of too few large adenomas (5.1% versus 14.% observed, with 95% confidence interval 11.8%–17.4%). Predictions were close to targets when we set sensitivities to 0.20 for diminutive adenomas, 0.60 for small adenomas, 0.80 for 10- to 20-mm adenomas, and 0.98 for adenomas 20 mm and larger.

Conclusions:

Colonoscopy may be less accurate than currently assumed, especially for diminutive adenomas. Alternatively, the CRC-SPIN model may not accurately simulate onset and progression of adenomas in higher-risk populations.

Impact:

Misspecification of either colonoscopy sensitivity or disease progression in high-risk populations may affect the predicted effectiveness of colorectal cancer screening. When possible, decision analyses used to inform policy should address these uncertainties.

See related commentary by Etzioni and Lange, p. 702
Transcription Factor FOSL1 Enhances Drug Resistance of Breast Cancer through DUSP7-Mediated Dephosphorylation of PEA15
Authors: Li Lin , Wang Nan , Xiong Youyi , Guo Guangcheng , Zhu Mingzhi , Gu Yuanting
Published: December 2021

Breast cancer is one of the commonest malignancies in women with first occurrence and fifth mortality in the world. However, drug resistance has always been a major obstacle to cancer treatment. Transcription factors have been reported to have close association with drug resistance of tumors. Recently, by analyzing the data from Gene Expression Omnibus database (GSE76540), we found that transcription factor FOS like 1, AP-1 transcription factor subunit (FOSL1) was significantly upregulated in the transcriptome of doxorubicin-resistant breast cancer cells compared with that in sensitive parental cells. Therefore, we aim to explore the regulatory mechanism of FOSL1 in affecting the drug resistance of breast cancer cells. FOSL1 expression in doxorubicin-resistant breast cancer cells was firstly examined through qRT-PCR, and then its influence on the drug resistance of breast cancer cells was explored through a series of in vitro and in vivo mechanism assays. Results showed that FOSL1 promoted the drug resistance of breast cancer cells to doxorubicin both in intro and in vivo. It positively regulated the transcription of dual specificity phosphatase 7 (DUSP7) in breast cancer doxorubicin-resistant cells and DUSP7 also enhanced the drug resistance of breast cancer cells. Furthermore, FOSL1 promoted the dephosphorylation of proliferation and apoptosis adaptor protein 15 (PEA15) through DUSP7. In conclusion, it was verified that FOSL1 promoted the drug resistance in breast cancer through DUSP7-mediated dephosphorylation of PEA15.

Implications:

These initial findings suggest that the FOSL1/DUSP7/PEA15 pathway may provide a theoretical guidance for breast cancer treatment.
Variant Identification in BARD1, PRDM9, RCC1, and RECQL in Patients with Ovarian Cancer by Targeted Next-generation Sequencing of DNA Pools
Authors: Suszynska Malwina , Ratajska Magdalena , Galka-Marciniak Paulina , Ryszkowska Aleksandra , Wydra Dariusz , Debniak Jaroslaw , Jasiak Anna , Wasag Bartosz , Cybulski Cezary , Kozlowski Piotr
Published: December 2021

Several ovarian cancer susceptibility genes have been discovered, but more are likely to exist. In this study, we aimed to analyze knowledge-based selected genes, that is, BARD1, PRDM9, RCC1, and RECQL, in which pathogenic germline variants have been reported in patients with breast and/or ovarian cancer. As deep sequencing of DNA samples remains costly, targeted next-generation sequencing of DNA pools was utilized to screen the exons of BARD1, PRDM9, RCC1, and RECQL in approximately 400 Polish ovarian cancer cases. A total of 25 pools of 16 samples (including several duplicated samples with known variants) were sequenced on the NovaSeq6000 and analyzed with SureCall (Agilent) application. The set of variants was filtrated to exclude spurious variants, and, subsequently, the identified rare genetic variants were validated using Sanger sequencing. No pathogenic mutation was found within the analyzed cohort of patients with ovarian cancer. Validation genotyping of filtered rare silent and missense variants revealed that the majority of them were true alterations, especially those with a higher mutation quality value. The high concordance (R2 = 0.95) of population allele frequency for 44 common SNPs in the European control population (gnomAD) and our experiment confirmed the reliability of pooled sequencing. Mutations in BARD1, PRDM9, RCC1, and RECQL do not contribute substantially to the risk of ovarian cancer. Pooled DNA sequencing is a cost-effective and reliable method for the initial screening of candidate genes; however, it still requires validation of identified rare variants.

Prevention Relevance:

BARD1, PRDM9, RCC1, and RECQL are not high/moderate-risk ovarian cancer susceptibility genes. Pooled sequencing is a reliable and cost-effective method to detect rare variants in candidate genes.
Importance of Family History in the Era of Exome Analysis: A Report of a Family with Multiple Concurrent Genetic Diseases
Authors: Mahtani Karishma , Park Diana , Abbott Jessica , Selvam Pavalan Panneer , Atwal Paldeep S.
Published: December 2021

Multiple familial diseases in a single patient often present with overlapping symptomatology that confers difficulty in delineating a clinical diagnosis. Pedigree analysis has been a long-standing practice in the field of medical genetics to discover familial diseases. In recent years, whole exome sequencing (WES) has proven to be a useful tool for aiding physicians in diagnosing and understanding disease etiology. This report shows that pedigree analysis and WES are co-dependent processes in establishing diagnoses in a family with 4 different genetic disorders: Birt-Hogg-Dubé Syndrome, RRM2B-related mitochondrial disease, CDC73-related primary hyperparathyroidism, and familial prostate cancer.
Mepolizumab Is an Effective Option in Severe Eosinophilic Asthma Regardless of Baseline Features: Single-Center Real-Life Data
Authors: Özdel Öztürk Betül , Yavuz Zeynep , Eraslan Dilek , Mungan Dilşad , Demirel Yavuz Selim , Aydın Ömür , Sin Betül Ayşe , Bavbek Sevim
Published: December 2021

Background: Mepolizumab has been approved as a treatment option for severe eosinophilic asthma (SEA) patients in our country. We aimed to evaluate the clinical and functional efficacy of mepolizumab in this group of patients in real life as well as the response rates to mepolizumab and the possible factors affecting the response. Methods: The study was a retrospective chart review of patients with SEA treated with mepolizumab. The data were collected at baseline, and at the 6th and 12th month. Results: A total of 62 patients (41F/21M) with a mean age of 44.41 ± 13.24 years were included in the study. They had poor symptom control with a mean asthma control test (ACT) score of 16.61 ± 5.59, frequent exacerbations with a mean of 3.4 ± 3.7 in the previous 12 months, and 80.6% required daily oral corticosteroid (OCS) with a median dosage of 8 mg/day as methylprednisolone. The ACT score increased to 22.47 ± 3.18 and 22.03 ± 4.31, respectively, and blood eosinophil count decreased from 1,146/μL to 89/μL and 85/μL at the 6th and 12th month, respectively. The mean FEV1 at baseline was 2.102 L there was an increase of 0.373 L at 6th month and 0.596 L at 12th month. The percentage of regular users of OCS decreased to 66.0% at 6th month with a median dosage of 4 mg and 52.6% at 12th month with a median dosage of 2 mg. Mepolizumab reduced the rate of exacerbations compared with the previous year from a mean of 3.40 to 0.15 at 6th month and 0.36 at 12th month. There was a significant improvement in Asthma Quality of Life Questionnaire (AQLQ), Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ), and Sino-nasal Outcome Test (SNOT-22) scores at both of time points. The rate of responders and super-responders at 6th month was 60% and 28%, respectively, and consequently, the overall response rate was 88%. At the 12th month, the super-responder rate increased to 44.7% as well as the overall response to 89.4%. The only difference between the nonresponders, responders, and super-responders at the 6th and 12th month was whether regular daily OCS was used pre-mepolizumab. All nonresponders at both 6th and 12th month were using OCS regularly, whereas most of super-responder used the OCS only during exacerbations. Conclusion: Mepolizumab effectively reduced asthma exacerbations, steroid requirement, blood eosinophil counts and improved asthma control, pulmonary function, sinonasal symptoms and quality of life. Our data suggest that mepolizumab would be effective in selected patients in real-life settings.
ОРГАНІЗАЦІЯ ІМУНОПРОФІЛАКТИКИ КЕРОВАНИХ РЕ-ЕМЕРДЖЕНТНИХ ІНФЕКЦІЙ В УМОВАХ ПАНДЕМІЇ SARS-COV-2
Authors: Волянська Л. А. , Боярчук О. Р. , Бурбела Е. І. , Павельєва М. М.
Published: December 2021

Своєчасна вакцинація – це однин із найуспішніших профілактичних напрямків охорони здоров’я, найефективніший і найдешевший спосіб для держави зберегти здоров’я своїх громадян.

Мета – запропонувати алгоритм організації планової імунізації дітей в умовах карантину для підвищення ефективності рутинної імунізації на первинній ланці та зниження ймовірності виникнення епідемічних спалахів ре-емерджентних інфекцій.

Пандемія SARS-CoV-2 внесла свої корективи в реалізацію налагоджених національних імунізаційних програм. Світ зіштовхнувся із кризою громадського здоров’я, ініційованого COVID-19, наслідком якої можуть стати значні соціально-економічні втрати через зростання ймовірності виникнення ре-емерджентних інфекцій і що може мати руйнівний вплив на кожен аспект людського життя, спричинивши значну захворюваність та смертність. ВООЗ рекомендує продовжувати планові щеплення в умовах пандемії, але з чітким дотриманням всіх протиепідемічних заходів. В умовах медичної реформи в Україні реалістичним є сценарій, який передбачає детальний аналіз стану колективного імунітету дільниці та об’єму потреб в імунізації для дітей цього підрозділу з подальшим сортуванням отриманих даних за термінами проведення, використанням певних вакцин, можливостями батьків відвідати лікаря в призначену дату. Окремо варто скористуватись можливістю поєднання планового патронажу дитини з вакцинацією, надати пріоритетність первинним вакцинальним комплексам і застосуванню комбінованих вакцин у формуванні індивідуального календаря імунізації для кожної дитини, що наздоганяє Календар. Програма наздоганяння вимагає визначити, хто пропустив, виявити, що пропустив та скласти відповідно отриманій інформації індивідуальні програми імунізації, враховуючи наявний перелік імунобіологічних препаратів за кошт держбюджету чи виходячи з можливостей або бажання батьків.

Висновок. Щеплення продовжують відігравати важливу роль у захисті вразливих людей від потенційно смертоносних хвороб. Незважаючи на те, що пандемія порушила важко здобутий прогрес, досягнутий за останнє десятиліття шляхом вакцинації, вона повинна тривати. Потрібно наздогнати втрачене шляхом створення індивідуальних схем рутинної імунізації, зробивши їх максимально зручними для батьків. Регулярні профілактичні щеплення як захід забезпечення громадського здоров’я є найбільш ефективним засобом у запобіганні виникненню ре-емерджентних інфекцій та економічно вигідним при запобіганні масової захворюваності та передчасної смертності. Запобіжна масова захворюваність і висока смертність шляхом регулярної рутинної імунізації перевищує за користю ймовірні ризики від COVID-19, пов’язані з відвідуванням клінік для вакцинації, особливо для дітей з уже наявним певним вакцинальним анамнезом.
ВПЛИВ КУРІННЯ НА РІВНІ АРГІНАЗИ ТА НІТРИТІВ І НІТРАТІВ У ПАЦІЄНТІВ З АРТЕРІАЛЬНОЮ ГІПЕРТЕНЗІЄЮ ТА ОЖИРІННЯМ
Authors: Покровська Н. К. , Файник А. Ф. , Скляров Є. Я.
Published: December 2021

Артеріальна гіпертензія залишається одним із найпоширеніших захворювань та одним із основних факторів розвитку кардіоваскулярних подій не лише в Україні, а й у світі. Активне та пасивне куріння є одними з головних факторів ризику серцево-судинної захворюваності та смертності, а хронічне куріння асоційовано з підвищеною артеріальною жорсткістю та зниженням еластичності артерій великого та середнього калібру.
Мета – з’ясувати вплив куріння на рівень аргінази та нітратів і нітритів (NO2- + NO3-) у пацієнтів з артеріальною гіпертензією та коморбідним ожирінням.
Матеріали та методи. Обстежено 56 пацієнтів віком 42–75 років із артеріальною гіпертензією та надмірною масою тіла чи ожирінням, з них 19 (33,9 %) пацієнтів курили. Пацієнтів було поділено на 2 групи залежно від анамнезу куріння. Було проведено такі обстеження: біохімічний аналіз крові, визначення рівнів аргінази та NO2- +NO3- у плазмі.
Результати. Знайдено зворотну кореляцію між індексом куріння та SpO2 (p<0,05), яка була достовірно нижчою у осіб, що курили (p<0,01). У пацієнтів із анамнезом куріння відмічено достовірне підвищення концентрації аргінази (p<0,05), а також зниження рівнів NO2- + NO3- (p<0,05). Знайдено зворотну кореляцію між рівнями NO2- + NO3-  та ШКФ (p<0,05) у осіб 2-ї групи, що дає можливість розглядати ці молекули в якості маркерів ураження ниркової паренхіми.
Висновки. Рівень аргінази є вищим у пацієнтів з АГ у поєднанні з ожирінням, котрі курять, порівняно із особами без шкідливих звичок, в той час як концентрація NO2- + NO3- є достовірно нижчою. NO2- + NO3- можна розглядати в якості маркерів ураження функції нирок у пацієнтів з коморбідною патологією.
КЛІНІЧНІ АСПЕКТИ ДІАГНОСТИКИ КАЛЬЦИФІКУЮЧОГО ТЕНДИНІТУ ПЛЕЧА
Authors: Грубар Ю. О. , Грубар М. Ю. , Грубар Ю. Ю. , Цицюра Р. І.
Published: December 2021

Кальцифікуючий тендиніт є захворюванням плечового суглоба з характерним накопиченням гідроксиапатиту кальцію в сухожилля обертової манжети та супроводжується локальним запаленням і больовим синдромом, що суттєво впливає на якість життя пацієнтів.
Мета – визначити характерні клінічні симптоми та ефективність додаткових методів дослідження для покращення діагностики та визначення подальшої тактики лікування вказаної патології.
Матеріал і методи. Проведено обстеження 58 (17,90 %) пацієнтів з кальцифікуючим тендинітом сухожилків обертальної манжети плеча. Середній вік пацієнтів становив (43,7±1,6) років, чоловіків було 23 (39,65 %), жінок 35 (60,35 %). Обстеження пацієнтів проводили згідно з клінічними протоколами надання медичної допомоги. Вони включали загальноклінічні та інструментальні методи дослідження, а саме – огляд пацієнта, рентгенографію, сонографію, при показаннях – магніторезонансну томографію.
Результати. Маніфестація симптомів залежала від фази захворювання. Серед провідних симптомів виявилися больовий синдром та обмеження обсягу активних та пасивних рухів, особливо відведення у плечовому суглобі. Рентгенологічні ознаки кальцифікуючого тендиніту виявлено у 87,93 % пацієнтів. Сонографічні ознаки патології були виявлені практично в усіх випадках і вони залежали від типу осифіката. Магніторезонансна томографія проводилася за показаннями.
Висновки. При клінічному дослідженні пацієнтів провідними симптомами виявилися біль різної інтенсивності та обмеження обсягу активних і пасивних рухів у суглобі. Рентгенографія та сонографія дозволяють в повній мірі визначити розміри та локалізацію відкладень гідроксиапатиту кальцію в сухожилля обертальної манжети плеча та визначити подальшу лікувальну тактику.
НЕЙРОГУМОРАЛЬНІ ПОРУШЕННЯ У ХВОРИХ НА ХРОНІЧНИЙ КОРОНАРНИЙ СИНДРОМ, КОМОРБІДНИЙ З НЕАЛКОГОЛЬНОЮ ЖИРОВОЮ ХВОРОБОЮ ПЕЧІНКИ
Authors: Михайловська Н. С. , Міняйленко Л. Є. , Лісова О. О. , Кулинич Т. О. , Грицай А. В. , Шершньова О. В. , Стецюк І. О.
Published: December 2021

Неалкогольна жирова хвороба печінки розглядається як один із асоційованих з метаболічним синдромом станів, що значно погіршує перебіг хронічного коронарного синдрому (ХКС).

Мета – вивчити рівні адипоцитокінів, маркерів системного запалення та ендотеліальної дисфункції та їх взаємозв’язок з клінічним перебігом ХКС, коморбідної з неалкогольною жировою хворобою печінки (НАЖХП).

Матеріал і методи. У проспективне моноцентрове подвійне відкрите дослідження в паралельних групах залучено 120 хворих на ХКС: стабільну стенокардію напруження ІІ–ІІІ функціонального класу віком 60,0 (55,0; 64,0) років, з них 67 (55 %) чоловіки і 53 (45 %) жінки. До групи контролю увійшли 30 практично здорових осіб віком 59,0 (58,0; 66,0) років, серед них 14 (46 %) чоловіків і 16 (54 %) жінок). Визначення вмісту біомаркерів (адипонектину, резистину, інсуліну, асиметричного диметиларгініну (ADMA), С-реактивного протеїну) у сироватці крові виконувалося за допомогою імуноферментного аналізу.

Результати. У хворих на ХКС із НАЖХП похідні рівні біомаркерів вірогідно (р<0,05) відрізнялись від показників групи контролю: концентрація резистину була на 48 % вищою, адипонектину на 60 % нижчою; спостерігалось збільшення вмісту СРБ (у 8,9 раза) та ADMA (на 42 %). У хворих на ХКС без патології печінки рівні адипонектину і резистину суттєво не переважали аналогічні показники у здорових осіб, однак відзначено достовірно вищі рівні СРБ (у 3,1 раза) та ADMA (на 30 %). У хворих на ХКС, поєднану з НАЖХП, порівняно з пацієнтами з ХКС без структурно-функціональної патології печінки, встановлено значне (р<0,05) зменшення рівня адипонектину на 31,6 %, збільшення резистину на 27 %, ADMA на 21 % та СРБ у 2,81 раза. У хворих з неалкогольним стеатогепатитом (НАСГ) активність системного запалення була вірогідно більшою, порівняно з хворими зі стеатозом, що підтверджувалось підвищенням концентрації СРБ на 44 % (р<0,05). Виявлені кореляції відображають взаємозв’язок функціонального стану печінки з метаболічними та нейрогуморальними порушеннями.

Висновки. У хворих на ХКС, асоційовану з НАЖХП, спостерігається дисбаланс адипоцитокінів (зменшення концентрації адипонектину, збільшення рівня резистину та зменшення співвідношення адипонектину до резистину) порівняно з контрольною групою та з хворими на ХКС без патології печінки. Достовірне зростання сироваткового рівня ADMA та СРБ свідчить про наявність дисфункції ендотелію на тлі активації системного запалення і може вказувати на патогенетичне значення цих маркерів у розвитку та прогресуванні ХКС на тлі НАЖХП.
РОЛЬ ІНТЕРЛЕЙКІНУ-6 І ГАММА-ІНТЕРФЕРОНУ В РЕГУЛЯЦІЇ РЕПАРАТИВНИХ ПРОЦЕСІВ ХРОНІЧНИХ РАН ПРИ ВИКОРИСТАННІ ФОТОБІОМОДУЛЯЦІЙНОЇ ТЕРАПІЇ
Authors: Павлов С. Б. , Бабенко Н. М. , Кумечко М. В. , Кочкіна С. В. , Хлібосолова Т. А.
Published: December 2021

Актуальним завданням сьогодення є пошук нових методів лікування хронічних ран. Перспективним методом впливу на репараційний процес є використання фотобіомодуляційної (ФБМ) терапії.

Мета – вивчення ролі інтерлейкіну-6 (ІЛ-6) і гамма-інтерферону (ІФН-γ) в регуляції репаративних процесів хронічних ран при застосуванні ФБМ терапії в експерименті.

Матеріали і методи. Дослідження були проведені на 24 щурах лінії Вістар, яким були змодельовані хронічні рани. Тварин було поділено на дві групи – експериментальну та контрольну. Ранові дефекти тварин експериментальної групи піддавали дії ФБМ терапії один раз на добу протягом 5 днів. Використовували лазерний пристрій «Ліка-терапевт М» (м. Черкаси) у безперервному режимі при довжині хвилі 660 нм, вихідній потужності 50 мВт, щільності енергії 1 Дж/см2, час експозиції 60 с. Забір крові з серця проводили на 3 та 7 добу після оперативного втручання. Дослідження рівнів ІЛ-6 і IФН-γ в сироватці крові тварин проводилося методом імуноферментного аналізу.

Результати. Наше дослідження показало, що через 3 дні після індукції рани рівні ІЛ-6 у сироватці крові тварин експериментальної групи достовірно знижувалися (3,418±0,381 пг/мл, P<0,05), порівняно з аналогічними показниками контрольної групи (7,356±0,649 пг/мл). Концентрації IФН-γ у сироватці крові тварин обох груп на цьому терміні загоєння ран не відрізнялись.

Через 7 днів рівні IФН-γ у крові тварин, ранові дефекти яких підлягали впливу ФБМ терапії, були знижені (103,330±4,493 пг/мл, P<0,05), порівняно з концентрацією IФН-γ тварин контрольної групи (155,208±9,574 пг/мл). Рівні IЛ-6 у сироватці крові тварин досліджуваних груп не відрізнялися.

Висновки. Регуляція порушень репаративних процесів хронічних ран може здійснюватися за допомогою ФБМ терапії, хоча цей факт тісно пов'язаний з вибором таких параметрів, як доза, час впливу і довжина хвилі лазерного випромінювання. Зниження продукції IЛ-6 і IФН-γ на початкових стадіях загоєння пошкоджень можуть бути одним з механізмів, за допомогою якого ФБМ терапія стимулює процеси репарації хронічних ран.
Sex Differences in Presentation of Stroke: A Systematic Review and Meta-Analysis
Authors: Ali Mariam , van Os Hendrikus J.A. , van der Weerd Nelleke , Schoones Jan W. , Heymans Martijn W. , Kruyt Nyika D. , Visser Marieke C. , Wermer Marieke J.H.
Published: December 2021

Background and Purpose:

Women have worse outcomes than men after stroke. Differences in presentation may lead to misdiagnosis and, in part, explain these disparities. We investigated whether there are sex differences in clinical presentation of acute stroke or transient ischemic attack.

Methods:

We conducted a systematic review and meta-analysis according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. Inclusion criteria were (1) cohort, cross-sectional, case-control, or randomized controlled trial design; (2) admission for (suspicion of) ischemic or hemorrhagic stroke or transient ischemic attack; and (3) comparisons possible between sexes in ≥1 nonfocal or focal acute stroke symptom(s). A random-effects model was used for our analyses. We performed sensitivity and subanalyses to help explain heterogeneity and used the Newcastle-Ottawa Scale to assess bias.

Results:

We included 60 studies (n=582 844; 50% women). In women, headache (pooled odds ratio [OR], 1.24 [95% CI, 1.11–1.39]; I
2
=75.2%; 30 studies) occurred more frequently than in men with any type of stroke, as well as changes in consciousness/mental status (OR, 1.38 [95% CI, 1.19–1.61]; I
2
=95.0%; 17 studies) and coma/stupor (OR, 1.39 [95% CI, 1.25–1.55]; I
2
=27.0%; 13 studies). Aspecific or other neurological symptoms (nonrotatory dizziness and non-neurological symptoms) occurred less frequently in women (OR, 0.96 [95% CI, 0.94–0.97]; I
2
=0.1%; 5 studies). Overall, the presence of focal symptoms was not associated with sex (pooled OR, 1.03) although dysarthria (OR, 1.14 [95% CI, 1.04–1.24]; I
2
=48.6%; 11 studies) and vertigo (OR, 1.23 [95% CI, 1.13–1.34]; I
2
=44.0%; 8 studies) occurred more frequently, whereas symptoms of paresis/hemiparesis (OR, 0.73 [95% CI, 0.54–0.97]; I
2
=72.6%; 7 studies) and focal visual disturbances (OR, 0.83 [95% CI, 0.70–0.99]; I
2
=62.8%; 16 studies) occurred less frequently in women compared with men with any type of stroke. Most studies contained possible sources of bias.

Conclusions:

There may be substantive differences in nonfocal and focal stroke symptoms between men and women presenting with acute stroke or transient ischemic attack, but sufficiently high-quality studies are lacking. More studies are needed to address this because sex differences in presentation may lead to misdiagnosis and undertreatment.
The Spirit in Matthew
Authors: Charette Blaine
Published: December 2021

It is clear that the Spirit of God plays a very important role in Matthew’s account. It is also important to note that Matthew refers to the Spirit in ways that are distinctive. For example, among the evangelists only Matthew speaks of the Spirit of God, and Matthew is unique in the NT in referring to the Spirit of the Father. This manner of nuancing the Spirit is analogous to the varied ways in which Matthew describes the kingdom, a concept qualified by such terms as “heaven,” “God,” “Father,” and “Son of Man.” This similarity is appropriate since a further unique feature of the Gospel is that Matthew alone decisively associates the Spirit of God with the presence of the kingdom of God. This discussion will focus first on Jesus’s experience of the Spirit and then on the work of the Spirit in the redemptive or kingdom activity of Jesus.