bluuebunny/crossref_metadata_2025 huggingface dataset:
Variety-Driven Effect of Rhizosphere Microbial-Specific Recruitment on Drought Tolerance of Medicago ruthenica (L.)
Authors: Xing Jing , Fan Wenqiang , Wang Jiani , Shi Fengling
Published: November 2023

As one of the environmental factors that seriously affect plant growth and crop production, drought requires an efficient but environmentally neutral approach to mitigate its harm to plants. Soil microbiomes can interact with plants and soil to improve the adverse effects of drought. Medicago ruthenica (L.) is an excellent legume forage with strong drought tolerance, but the key role of microbes in fighting drought stress remains unclear. What kind of flora plays a key role? Is the recruitment of such flora related to its genotype? Therefore, we selected three varieties of M. ruthenica (L.) for drought treatment, analyzed their growth and development as well as their physiological and biochemical characteristics, and performed 16S rRNA high-throughput sequencing analysis on their rhizosphere soils to clarify the variety-mediated response of rhizosphere bacteria to drought stress. It was found that among the three varieties of M. ruthenica (L.), Mengnong No.2, Mengnong No.1 and Zhilixing were subjected to drought stress and showed a reduction in plant height increment of 24.86%, 34.37%, and 31.97% and in fresh weight of 39.19%, 50.22%, and 41.12%, respectively, whereas dry weight was reduced by 23.26%, 26.10%, and 24.49%, respectively. At the same time, we found that the rhizosphere microbial community of Mengnong No. 2 was also less affected by drought, and it was able to maintain the diversity of rhizosphere soil microflora stable after drought stress, while Mennong No. 1 and Zhilixing were affected by drought stress, resulting in a decrease in rhizosphere soil bacterial community diversity indices to 92.92% and 82.27%, respectively. Moreover, the rhizosphere of Mengnon No. 2 was enriched with more nitrogen-fixing bacteria Rhizobium than the other two varieties of M. ruthenica (L.), which made it still have a good ability to accumulate aboveground biomass after drought stress. In conclusion, this study proves that the enrichment process of bacteria is closely related to plant genotype, and different varieties enrich different types of bacteria in the rhizosphere to help them adapt to drought stress, and the respective effects are quite different. Our results provide new evidence for the study of bacteria to improve the tolerance of plants to drought stress and lay a foundation for the screening and study mechanism of drought-tolerant bacteria in the future.
Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand
Authors: Dokuta Sirikwan , Yadoung Sumed , Jeeno Peerapong , Hongjaisee Sayamon , Khamnoi Phadungkiat , Manochomphu Sirinya , Danmek Khanchai , Maitip Jakkrawut , Chuttong Bajaree , Hongsibsong Surat
Published: November 2023

This study aims to determine the prevalence of microorganisms and antibiotic-resistant microorganisms in beehives located on different plantations in Thailand. Seventeen swabs immersed in transport media were utilized for samples from different zones within beehives. Traditional microbial culture-based methods, biochemical tests, MALDI-TOF MS (VITEK® MS, bioMerieux, Marcy-l’Étoile, France), and antibiotic drug susceptibility (disk-diffusion) tests were used to detect microorganism and antimicrobial resistance bacteria. The results from 16 beehive swabs found Gram-positive bacteria at 59.5%, Gram-negative bacteria at 35.1%, and fungi (yeast) at 5.4%. These organisms are classified as 11, 11, and 2 types of Gram-positive bacteria, Gram-negative bacteria, and fungi (yeast), respectively. Furthermore, no organism showed resistance to vancomycin or cefoxitin for antibiotic drug susceptibility testing. In contrast, all Acinetobacter spp. were susceptible to ciprofloxacin, levofloxacin, ceftazidime, cefotaxime, imipenem, and meropenem, except for Acinetobacter schindleri, which was resistant to ceftazidime and cefotaxime. For other organisms, due to the limitations of tests to identify some environmental microbial species, the antimicrobial susceptibility test results cannot be interpreted as resistant or susceptible to the drug for these organisms. The study’s findings will support prevention, healthcare services, and public health systems.
Comparison of Frailty and Chronological Age as Determinants of the Murine Gut Microbiota in an Alzheimer’s Disease Mouse Model
Authors: Kapphan Laura Malina , Nguyen Vu Thu Thuy , Heinrich Isabel , Tüscher Oliver , Passauer Pamela , Schwiertz Andreas , Endres Kristina
Published: November 2023

The ageing of an organism is associated with certain features of functional decline that can be assessed at the cellular level (e.g., reduced telomere length, loss of proteostasis, etc.), but also at the organismic level. Frailty is an independent syndrome that involves increased multidimensional age-related deficits, heightens vulnerability to stressors, and involves physical deficits in mainly the locomotor/muscular capacity, but also in physical appearance and cognition. For sporadic Alzheimer’s disease, age per se is one of the most relevant risk factors, but frailty has also been associated with this disease. Therefore, we aimed to answer the two following questions within a cross-sectional study: (1) do Alzheimer’s model mice show increased frailty, and (2) what changes of the microbiota occur concerning chronological age or frailty? Indeed, aged 5xFAD mice showed increased frailty compared to wild type littermates. In addition, 5xFAD mice had significantly lower quantities of Bacteroides spp. when only considering frailty, and lower levels of Bacteroidetes in terms of both frailty and chronological age compared to their wild type littermates. Thus, the quality of ageing—as assessed by frailty measures—should be taken into account to unravel potential changes in the gut microbial community in Alzheimer’s disease.
Age and Petrogenesis of the Dongjin Rare Metal Mineralized Intrusion in the Northern Margin of the North China Craton
Authors: Liu Chenyu , Chen Gongzheng , Wang Jinfang , Cheng Yi , Li Kangshuo , Lu Zeqian , Song Yutong
Published: November 2023

Highly fractionated granites are widespread in the middle part of the northern margin of the North China Craton (MNNCC), and several are accompanied by rare metal mineralization. The Dongjin rare metal mineralized intrusion, which is representative of this region, is composed of fine-grained alkali-feldspar granite (FAG) and kali-feldspar granite (KG). The FAG and KG evolve continuously, exemplifying the relationship between magmatic evolution and rare metal mineralization. In this contribution, we present integrated columbite U-Pb geochronology, mineralogy, and whole-rock geochemistry analyses of the Dongjin intrusion to determine the timing of the mineralization, petrogenesis, and geodynamic setting, from which the following results are obtained: (1) LA-ICP-MS U-Pb dating for columbite of the FAG and KG yielded the lower intercept ages between 248.9 ± 1.9 Ma and 250.1 ± 1.1 Ma on the Tera–Wasserburg concordia diagram; (2) Geochemically, the Dongjin intrusion is characterized by an enrichment in Si, Al, Rb, Th, U, Nb, and Zr and a strong depletion in Ba, Sr, P, and Ti, with extremely negative Eu anomalies, high LREE and HREE values, and a noticeable tetrad effect of rare earth elements; as a result, it belongs to high-K calc-alkaline rocks; (3) The Dongjin intrusion belongs to a highly differentiated I-type or A-type granite; (4) The fractional crystallization of plagioclase, K-feldspar, and biotite occurred during magmatic evolution; (5) The Dongjin intrusion was formed in a post-collisional extensional environment. In conclusion, the FAG and KG have a homologous evolution, and the FAG has a higher degree of fractional crystallization. The enrichment and mineralization of Nb-Ta are related to the highly fractionated crystallization of granitic magma and fluid–melt interactions in the final stages of magmatic evolution, and there is a rare metal mineralization related to highly fractionated granite in the MNNCC in the Early Triassic, which deserves full attention in future research and prospecting.
Utilization of PMA-EDTC as a Novel Macromolecular Depressant for Galena in the Flotation Separation of Chalcopyrite
Authors: Zeng Hong , Zhu Yangge , Sun Chuanyao , Zhao Zhiqiang , Wu Guiye , Liu Chongjun , Lu Tong , Zhang Xingrong
Published: November 2023

To address the issue of mediocre separation efficiency of depressants in the copper-lead separation process, this article synthesized a macromolecular organic depressant, polymaleic anhydride-ethylenediaminetetraacetic acid (PMA–EDTC), using a polycarboxylic macromolecule as the backbone and also introducing –N–(C=S)–S– as the solidophilic group and employed as a galena depressant. The structure of PMA–EDTC was characterized using Fourier transform infrared (FT-IR). The effect of PMA–EDTC on the floatability of galena and chalcopyrite was investigated through micro-flotation and Contact angle measurements. The experimental results demonstrated that PMA–EDTC exhibited selectivity inhibition towards galena rather than chalcopyrite across a wide pH range. At a dose of 8 mg/L, there was effective separation between galena and chalcopyrite with a separation coefficient of 24.17, effectively altering the floatability of galena while having little impact on the floatability of chalcopyrite. The selective inhibition behavior and adsorption mechanism of PMA–EDTC on galena and chalcopyrite were investigated using FT-IR, Zeta potential, and X-ray photoelectron spectroscopy (XPS). FT-IR and Zeta potential studies indicated that PMA–EDTC formed chemical adsorption on the surface of galena. XPS confirmed the model of chemical adsorption of PMA–EDTC on lead atoms in the galena surface. The results indicate that PMA–EDTC adsorbs on the surface of galena via its –(C=S)–S–group, forming a hydrophilic complex and achieving selective depression of lead and the cleaning flotation of copper.
Thermodynamic and Kinetic Studies of Dolomite Formation: A Review
Authors: Chen Chao , Zhong Hanting , Wang Xinyu , Ning Meng , Wang Xia , Ge Yuzhu , Wang Han , Tang Ruifeng , Hou Mingcai
Published: November 2023

The “dolomite problem”, which has confused scientists for nearly two centuries, is an important fundamental geological problem. The mineralogical characteristics of carbonate minerals show that the dolomite structure consists of an ordered arrangement of alternating layers of Ca2+ and Mg2+ cations interspersed with CO32− anion layers normal to the c-axis. The dolomite structure violates the c glide plane in the calcite structure, which means that dolomite has R3¯ space group symmetry. The ordered dolomite has superlattice XRD reflections [e.g., (101), (015) and (021)], which distinguish it from calcite and high-Mg calcite. The calculation of thermodynamic parameters shows that modern seawater has a thermodynamic tendency of dolomite precipitation and the dolomitization reaction can be carried out in standard state. However, the latest thermodynamic study shows that modern seawater is not conducive to dolomitization, and that seawater is favorable for dolomitization in only a few regions, such as Abu Dhabi, the Mediterranean and the hypersaline lagoons in Brazil. The kinetic factors of dolomite formation mainly consist of the hydration of Mg2+, the presence of sulfate and the activity of carbonate. Current studies have shown that the presence of microorganisms, exopolymeric substances (EPS), organic molecules, carboxyl and hydroxyl functional groups associated with microorganisms and organic molecules, clay minerals with negative charges and dissolved silica facilitate magnesium ions to overcome hydration and thus promote Mg2+ incorporation into growing Ca-Mg carbonates. Similarly, the metabolic activity of microorganisms is conducive to the increase in alkalinity. However, the inhibitory effect of sulfate on dolomite formation seems to be overestimated, and sulfate may even be a catalyst for dolomite formation. Combining the carbonate crystallization mechanism with thermodynamic and kinetic factors suggests that the early stage of dolomite precipitation or the dolomitization reaction may be controlled by kinetics and dominated by unstable intermediate phases, while metastable intermediate phases later transform to ordered dolomite via an Ostwald’s step rule.
Electrical Prospecting of Gold Mineralization in Exhalites of the Digo-Digo VMS Occurrence, Central Brazil
Authors: do Amaral Pedro Augusto Costa , Borges Welitom Rodrigues , Toledo Catarina Labouré Bemfica , Silva Adalene Moreira , de Godoy Hygor Viana , Leão Santos Marcelo Henrique
Published: November 2023

The greenstone belts of the Crixás-Goiás Domain are economically important due to significant epigenetic gold deposits and the potential for under-researched syngenetic deposits. The gold occurrences associated with the volcanogenic massive sulfide (VMS) deposits in the region are documented only in the volcanoclastic rocks of the Digo-Digo Formation, Serra de Santa Rita greenstone belt. The objective of this work is to discuss the efficiency of the induced polarization methods in the time and frequency domains for differentiating and identifying potentially mineralized zones in the exhalites associated with the VMS-type gold of the Digo-Digo Formation. Data were acquired using a multielectrode resistivity meter with the dipole–dipole array and 10 m spacing between electrodes, as well as different current injection times (250, 1000, and 2000 ms). After the electrical data processing and inversion, the sections were integrated into ternary red-green-blue and cyan-magenta-yellow models to highlight areas of high chargeability, low resistivity, and high metal factor (frequency domain) and, thus, map the higher potential zones to host polarizable metallic minerals. The geological–geophysical model elaborated from the correlation of electrical and surface geological data allowed us to identify four anomalous areas related to potential mineralized zones. The geological data confirm that two targets are associated with the geological contacts between metamafic and intermediate metavolcanic units and the exhalative horizon. One of the targets coincides with a sulfide-rich exhalative horizon (VMS), while the last target occurs in the occurrence area of metaultramafic rocks, where gold mineralization occurrences have not been previously described, being a promising target for future investigations.
Comparison of the Analytical Performance of Inductively Coupled Plasma Mass Spectrometry and Instrumental Neutron Activation Analysis Techniques in the Elemental Analysis of Coal Fly Ash
Authors: Chajduk Ewelina , Kalbarczyk Paweł
Published: November 2023

Inductively coupled plasma mass spectrometry (ICP-MS) and instrumental neutron activation analysis (INAA) were applied to study the elemental content of coal combustion residues. Both methods’ analytical applicability and suitability for this type of material were described in detail. A certified reference material, fine fly ash (CTA-FFA-1), was used to examine the capabilities and validation of both methods. A comparison study allowed for the modification of the procedures for these materials (previously elaborated in the Institute of Nuclear Chemistry and Technology), resulting in more accurate data with lower uncertainty. We highlighted that INAA and ICPs complemented each other in the analysis of coal and coal combustion residues. The digestion procedure was an integral part of the measurement and affected the environmental analysis results. Both methods were applied to the chemical characteristics of coal ashes from household furnaces. The hard coal was from Polish, Columbian, and Kazakh mines. The majority of elements had similar concentrations, independently of coal origin. Because ash from domestic coal combustion is used as a fertilizer or an anti-icing agent, we assessed the potential environmental impact via leaching experiments (according to PN-EN 12457-2). The potential risk of soil contamination due to ash dumping around the house and its surroundings was determined as insignificant (for the elements investigated).
Computational Modeling Study of the Binding of Aging and Non-Aging Inhibitors with Neuropathy Target Esterase
Authors: Wu Wenxiong , Wang Pan
Published: November 2023

Neuropathy target esterase (NTE) is a serine hydrolase with phospholipase B activity, which is involved in maintaining the homeostasis of phospholipids. It can be inhibited by aging inhibitors such as some organophosphorus (OP) compounds, which leads to delayed neurotoxicity with distal degeneration of axons. However, the detailed binding conformation of aging and non-aging inhibitors with NTE is not known. In this study, new computational models were constructed by using MODELLER 10.3 and AlphaFold2 to further investigate the inhibition mechanism of aging and non-aging compounds using molecular docking. The results show that the non-aging compounds bind the hydrophobic pocket much deeper than aging compounds and form the hydrophobic interaction with Phe1066. Therefore, the unique binding conformation of non-aging compounds may prevent the aging reaction. These important differences of the binding conformations of aging and non-aging inhibitors with NTE may help explain their different inhibition mechanism and the protection of non-aging NTE inhibitors against delayed neuropathy.
Chemical Composition of Essential Oils and Supercritical Carbon Dioxide Extracts from Amomum kravanh, Citrus hystrix and Piper nigrum ‘Kampot’
Authors: Katerina Vihanova , Klara Urbanova , Samnang Nguon , Ladislav Kokoska
Published: November 2023

The fruits of Amomum kravanh, Citrus hystrix and Piper nigrum ‘Kampot’ are traditionally used as spices in Cambodian cuisine. In this study, the chemical composition of essential oils (EOs) and supercritical CO2 extracts from all three species was determined using GC-MS, with two columns of different polarity (HP-5/DB-HeavyWAX). Differences between the chemical profile of the EOs and CO2 extracts were observed for all species. The greatest difference was detected in A. kravanh EO containing mainly eucalyptol (78.8/72.6%), while the CO2 extract was rich in fatty acids (13/55.92%) and long-chain alkanes (25.55/9.54%). Furthermore, the results for the CO2 extract of this species differed, where tricosane (14.74%) and oleic acid (29.26%) were the main compounds identified when utilizing the HP-5 or DB-HeavyWAX columns, respectively. Moreover, the EO and CO2 extract from P. nigrum ‘Kampot’ fruits and the CO2 extract from C. hystrix fruit peel, containing respective amounts 34.84/39.55% (for EO) and 54.21/55.86% (for CO2 extract) of β-caryophyllene and 30.2/28.9% of β-pinene, were isolated and analyzed for the first time. Generally, these findings suggest that supercritical CO2 could potentially be used for the extraction of all three spices. Nevertheless, further research determining the most efficient extraction parameters is required before its commercial application.
Local Clays from China as Alternative Hemostatic Agents
Authors: Gan Changjiao , Hu Hongjie , Meng Zhiyun , Zhu Xiaoxia , Gu Ruolan , Wu Zhuona , Sun Wenzhong , Han Peng , Wang Hongliang , Dou Guifang , Gan Hui
Published: November 2023

In recent years, the coagulation properties of inorganic minerals such as kaolin and zeolite have been demonstrated. This study aimed to assess the hemostatic properties of three local clays from China: natural kaolin from Hainan, natural halloysite from Yunnan, and zeolite synthesized by our group. The physical and chemical properties, blood coagulation performance, and cell biocompatibility of the three materials were tested. The studied materials were characterized by using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). All three clays showed different morphologies and particle size, and exhibited negative potentials between pH 6 and 8. The TGA and DSC curves for kaolin and halloysite were highly similar. Kaolin showed the highest water absorption capacity (approximately 93.8% ± 0.8%). All three clays were noncytotoxic toward L929 mouse fibroblasts. Kaolin and halloysite showed blood coagulation effects similar to that exhibited by zeolite, indicating that kaolin and halloysite are promising alternative hemostatic materials.
Roughness Factors of Electrodeposited Nanostructured Copper Foams
Authors: Levin Eduard E. , Morozov Dmitriy A. , Frolov Vsevolod V. , Arkharova Natalia A. , Khmelenin Dmitry N. , Antipov Evgeny V. , Nikitina Victoria A.
Published: November 2023

Copper-based electrocatalytic materials play a critical role in various electrocatalytic processes, including the electroreduction of carbon dioxide and nitrate. Three-dimensional nanostructured electrodes are particularly advantageous for electrocatalytic applications due to their large surface area, which facilitates charge transfer and mass transport. However, the real surface area (RSA) of electrocatalysts is a crucial parameter that is often overlooked in experimental studies of high-surface-area copper electrodes. In this study, we investigate the roughness factors of electrodeposited copper foams with varying thicknesses and morphologies, obtained using the hydrogen bubble dynamic template technique. Underpotential deposition (UPD) of metal adatoms is one of the most reliable methods for estimating the RSA of highly dispersed catalysts. We aim to illustrate the applicability of UPD of lead for the determination of the RSA of copper deposits with hierarchical porosity. To find the appropriate experimental conditions that allow for efficient minimization of the limitations related to the slow diffusion of lead ions in the pores of the material and background currents of the reduction of traces of oxygen, we explore the effect of lead ion concentration, stirring rate, scan rate, monolayer deposition time and solution pH on the accuracy of RSA estimates. Under the optimized measurement conditions, Pb UPD allowed to estimate roughness factors as high as 400 for 100 µm thick foams, which translates into a specific surface area of ~6 m2·g−1. The proposed measurement protocol may be further applied to estimate the RSA of copper deposits with similar or higher roughness.
Remarkable Magnetic Properties in a Mn73.6Ga26.4 Alloy Produced via Out-of-Equilibrium Method
Authors: Crisan Ovidiu , Crisan Alina Daniela
Published: November 2023

Rare-earth-free permanent magnets with the L10 phase are actively researched for their potential as a future class of magnetic materials, capable of operating at higher temperatures and in challenging corrosion environments such as renewable energy applications. Among these classes, MnGa shows potential, being cost effective and having interesting magnetic properties. A MnGa magnetic alloy, with composition Mn73.6Ga26.4 in atomic percent, was produced via the out-of-equilibrium method, and its structural and magnetic properties were assessed using X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and extended magnetic characterization. We show that the MnGa alloy submitted to thermal annealing in optimal conditions exhibits a two-phase microstructure, where small nanocrystals of tetragonal L10/D022 magnetic phase are embedded within a D019 MnGa matrix of a non-collinear antiferromagnetic nature. These co-existing, magnetically different phases produce an optimal set of promising magnetic properties, larger than the values reported in the literature for single-phase MnGa alloys and thin films. Such large values are explained by the exchange coupling between competing non-collinear magnetic sublattices of the D019 MnGa with the net moment of the small magnetic nanocrystals of tetragonal symmetry.
Advances in Natural and Bio-Inspired Nanoparticles for the Treatment of Cardiovascular Diseases
Authors: Varna Mariana , Calin Manuela , Gebeshuber Ille C.
Published: November 2023

Cardiovascular diseases (CVD) is a general term for disorders affecting the heart or blood vessels and represent a major cause of disability and death worldwide [...]
Physical Exercise or Activity and Energy Balance or Metabolism in the Context of Health and Diseases
Authors: Messonnier Laurent A.
Published: November 2023

Regular long-lasting physical exercise demands a tremendous amount of metabolic energy [...]
Effectiveness of Time-Restricted Eating with Caloric Restriction vs. Caloric Restriction for Weight Loss and Health: Meta-Analysis
Authors: Črešnovar Tanja , Habe Bernarda , Jenko Pražnikar Zala , Petelin Ana
Published: November 2023

Time-restricted eating (TRE) is an increasingly popular dietary strategy for weight loss. Recent studies suggest that combining TRE with caloric restriction (CR) may have more favorable effects on both physical and biochemical aspects when compared with CR alone. Therefore, we performed a meta-analysis to compare the effects of TRE with CR vs. CR alone on anthropometric and biochemical measures in overweight or obese adults. We reviewed articles from PubMed, Web of science, EMBASE, and the Cochrane Library published before 25 May 2023. The meta-analysis incorporated data from seven randomized controlled trials of nine interventions, with a total of 231 participants in the TRE with CR group and 227 participants in the CR-only group. Data were analyzed using RewMan version 5.4.1. All results in our meta-analysis were described as mean difference (MD) with 95% confidence interval (Cl). Results showed that TRE with CR compared to CR alone resulted in significantly greater reductions in body weight (MD: −2.11 kg, 95% CI: −2.68 kg to −1.54 kg, p = < 0.00001, I2 = 42%), body fat mass (MD: −0.75 kg, 95% CI: −1.35 kg to −0.16 kg, p = 0.01; I2 = 0%), and waist circumference (MD: −1.27 cm, 95% CI: −2.36 cm to −0.19 cm, p = 0.02, I2 = 0%), while no additional impact of TRE in combination with CR in comparison to CR on serum biochemical parameters were found. Our results suggest that the improvement in biochemical parameters are mainly caused by CR, while improvements in anthropometric parameters are further enhanced by TRE.
Theabrownin from Wuniuzao Dark Tea Regulates Hepatic Lipid Metabolism and Gut Microbiota in Mice Fed High-Fat Diet
Authors: Xu Qianqian , Ye Jiangcheng , Gong Mingxiu , Zhang Yifan , Yuan Yiwei , Zhao Jin
Published: November 2023

The search for functional foods with no side effects that can alleviate obesity has been a common trend. Wuniuzao dark tea could be a safe choice. This study aimed to explore whether theabrownin from Wuniuzao dark tea could regulate hepatic lipid metabolism and gut microbiota in mice fed a high-fat diet. In total, fifty 8-week-old male C57BL/6 mice were randomly divided into five treatment groups, including a normal control group, high-fat diet group, positive control group, low-dose theabrownin group, and high-dose theabrownin group. After a 9-week intervention, these mice were selected from each treatment group for sampling. The results showed that the body weight and epididymis fat weight of obese mice fed with theabrownin were decreased. Serum total triglycerides, total cholesterol, and low-density lipoprotein cholesterol, and activities of aspartate aminotransferase and alanine aminotransferase were also decreased. Protein and mRNA expression of fatty acid synthesis and lipid production-related genes of mice fed with theabrownin were downregulated. The gut microbiota composition in the theabrownin group was improved. The study indicated that theabrownin from Wuniuzao dark tea could achieve the liver protection and anti-obesity effects by regulating the Srebp lipid metabolism pathway and bile acid metabolism process, and improving the gut microbiota composition of mice.
Prevalence of Sarcopenia and Dynapenia and Related Clinical Outcomes in Patients with Type 1 Diabetes Mellitus
Authors: Andreo-López María Carmen , Zarco-Martín María Teresa , Contreras-Bolívar Victoria , Fernández-Soto María Luisa
Published: November 2023

Background: Sarcopenia has recently been recognized as a complication of diabetes. However, there are few results about the prevalence of sarcopenia and dynapenia and the related clinical outcomes in type 1 diabetes mellitus (T1DM). Our objectives were to evaluate the prevalence of sarcopenia and dynapenia and to determine whether there are any associations with disease-related factors in people with T1DM. Methods: A cross-sectional study was conducted in people with T1DM. We assessed appendicular skeletal mass index (ASMI) using bioimpedance 50 Hz (Nutrilab Akern). Muscle function was assessed through handgrip strength (HGS) using a Jamar dynamometer. Sarcopenia was defined as a low HGS with low ASMI, whereas dynapenia was defined as low HGS with a normal ASMI. We used HGS data from the Spanish population percentile table and a cut-off point at p5 as dynapenia. The association of clinical, metabolic, and lifestyle variables with sarcopenia and dynapenia was studied. Results: This study included 62 T1DM patients (66% females, mean age of 38 ± 14 years, body mass index (BMI) of 24.9 ± 4.7 kg/m2). The prevalence of sarcopenia and dynapenia was 8% and 23%, respectively. In our sample, there were more men in the sarcopenic and dynapenic groups. The sarcopenic group showed a significantly higher mean HbA1c value. Lower diabetes duration, PREDIMED score, BMI, and muscle mass measures (fat-free mass index (FFMI), ASMI, and body cell mass index (BCMI)) were significantly associated with sarcopenia. Decreased diabetes duration, PREDIMED score, phase angle (PhA), and HGS values showed a significant association with dynapenia. Conclusions: The prevalence of sarcopenia and dynapenia was high in people with T1DM in our study. Specifically, the proportion of dynapenia was quite high. HGS and ASMI are practical tools for the assessment of muscle health status in T1DM, and low values are associated with poor glycemic control, underweight, and low adherence to the Mediterranean diet. Thus, dynapenia may predict accelerated muscle aging in T1DM.
Numerical Study of the Spectrum of TE-Polarized Electromagnetic Waves of a Goubau Line Coated with Graphene
Authors: Smolkin Eugen , Smirnov Yury
Published: November 2023

The problem of TE-polarized waves in a Goubau line (a perfectly conducting cylinder covered by a concentric layer) coated with graphene is studied. The classification of the waves existing in a Goubau line is carried out. The physical problem is reduced to solving a transmission eigenvalue problem for an ordinary differential equation. The conjugation conditions contain the conductivity of graphene. In this work, we take into account the nonlinearity of graphene. Spectral parameters of the problem are the propagation constants of the waveguide. The article proposes a numerical method for calculating the propagation constants of such waves. A number of numerical experiments were carried out with a Goubau line filled with a dielectric, inhomogeneous dielectric, dielectric with losses, and metamaterial.
Effect of Beam-Steering Angle, Operation Wavelength and Mean Inter-Element Distance on the Side-Lobe Levels of Integrated Optical Phased Arrays under Beam-Steering Operation
Authors: Aldaya Ivan , Pires de Souza Bruna Dias , Penchel Rafael Abrantes , Pita Julian Leonel , dos Santos Mirian Paula , de Oliveira José Augusto , Abbade Marcelo Luis Francisco
Published: November 2023

Integrated optical phased arrays (OPAs) play an important role in a broad range of applications. Fabrication constraints, however, pose a limit to the minimum inter-element separation that further results in high-intensity side lobes. The intensity of these secondary lobes can be reduced by arranging the antenna elements with non-uniform separation distance, which has been addressed by different methods. In this paper we employ one of the already proven optimization algorithms, i.e., differential evolution, to optimize the element positions of linear arrays with different configurations operating under beam-steering operation and considering a minimum inter-element distance. These optimizations allowed us to derive some design guidelines that can assist in reducing the side-lobe level (SLL) of integrated linear OPAs. In particular, we found that it is necessary to optimize the positions for the broadest beam-steering angle and the shortest operation wavelength. Additionally, optimizations of different configurations reveal that, when imposing a minimum inter-element distance, there is an optimum mean distance that minimizes the SLL of the array.
A Travel through Landscapes of Seed Dormancy
Authors: Gianinetti Alberto
Published: November 2023

Basic features of seed dormancy are illustrated. The seed overall regulatory network governs seed metabolism and development, and it is coordinated by plant hormones. A functional model focused on abscisic acid (ABA), the foremost plant hormone in dormancy, is used as a framework to critically discuss the literature. Gibberellins (GAs) have a main role in germination, and the ABA–GAs balance is a typical feature of the seed state: ABA dominates during dormancy and GAs prevail through germination. Thus, the literature converges toward envisaging the development switch between dormancy and germination as represented by the ABA/GAs ratio. The ABA–GAs antagonism is based on mutual inhibition, a feature of the regulatory network architecture that characterizes development trajectories based on a regulatory circuit with a bistable switch. Properties of such kind of regulatory architecture are introduced step by step, and it is shown that seed development—toward either dormancy or germination—is more properly represented by a tristable regulatory circuit, whose intermediate metastable states ultimately take one or the other development trajectory. Although the ABA/GAs ratio can conveniently represent the state of the seed overall regulatory network along the seed development trajectory, specific (unknown) dormancy factors are required to determine the development trajectory. The development landscape is shown to provide a well-suited representation of seed states travelling along developmental trajectories, particularly when the states are envisioned as regulatory circuits. Looking at seed dormancy in terms of regulatory circuits and development landscapes offers a valuable perspective to improve our understanding of this biological phenomenon.
Changes in Mechanical Properties of Fabrics Made of Standard and Recycled Polyester Yarns Due to Aging
Authors: Katić Križmančić Ines , Salopek Čubrić Ivana , Potočić Matković Vesna Marija , Čubrić Goran
Published: November 2023

Over the years, the demands on the durability and quality of polyester fabrics used for sportswear have increased, as these fabrics contribute to athletes’ performance. At the same time, the use of recycled polyester material is increasingly being promoted for environmental reasons. This study focused on investigating the properties of standard and recycled polyester fabrics before and after aging according to the developed aging protocol. The surface morphology, thickness, elongation at break, force at break, bursting force, mass loss due to abrasion and moisture management of the fabrics were tested. The results showed that the aging process had no influence on the surface changes in the fabrics. More specifically, there were neither surface cracks on the fibre surface nor chemical changes. The highest decrease in force at break for standard polyester fabrics with elastane was up to 26%, and up to 15% for fabrics made of recycled polyester. The loss of mass due to abrasion was greater for recycled polyester than for standard polyester fabrics. The average ability of the fabrics to absorb moisture decreased by up to 23% after aging, while the wetting time increased by up to 30%, with the highest increase observed in recycled fabrics.
Experimental Study on Enhanced Oil Recovery Effect of Profile Control System-Assisted Steam Flooding
Authors: Dong Long , Zhao Fajun , Zhang Huili , Liu Yongjian , Huang Qingyu , Liu Da , Guo Siqi , Meng Fankun
Published: November 2023

Steam flooding is an effective development method for heavy oil reservoirs, and the steam flooding assisted by the profile control system can plug the dominant channels and further improve the recovery factor. High-temperature-resistant foam as a profile control system is a hot research topic, and the key lies in the optimal design of the foam system. In this paper, lignin was modified by sulfonation to obtain a high-temperature-resistant modified lignin named CRF; the foaming agent CX-5 was confirmed to have good high-temperature foaming ability by reducing the surface tension; the formula of the profile control system (A compound system of CRF and CX-5, abbreviated as PCS) and the best application parameters were optimized by the foam resistance factor. Finally, the effect of PCS-assisted steam flooding in enhanced oil recovery was evaluated by single sand packing tube flooding, three parallel tube flooding, and large-scale sand packing model flooding experiments. The results show that CX-5 has a good high-temperature foaming performance; the foam volume can reach more than 180 mL at 300 °C, and the half-life is more than 300 s. The optimal PCS formulation is 0.3 wt% CRF as an oil displacement agent + 0.5 wt% CX-5 as a foaming agent. The optimal gas–liquid ratio range is 1:2 to 2:1, and the high pressure and permeability are more conducive to the generation and stability of the foam. Compared with steam flooding, PCS-assisted steam flooding can improve oil recovery by 9% and 7.9% at 200 °C and 270 °C, respectively. PCS can effectively improve the heterogeneity of the reservoir, and increase the oil recovery of the three-parallel tube flooding experiment by 28.7%. Finally, the displacement results of the sand-packing model with large dimensions show that PCS can also expand the swept volume of the homogeneous model, but the effect is 9.46% worse than that of the heterogeneous model.
Recent Advances in 3D Printing of Polymers for Application in Prosthodontics
Authors: Dimitrova Mariya , Vlahova Angelina , Kalachev Yavor , Zlatev Stefan , Kazakova Rada , Capodiferro Saverio
Published: November 2023

Contemporary mass media frequently depict 3D printing as a technology with widespread utilization in the creation of dental prosthetics. This paper endeavors to provide an evidence-based assessment of the current scope of 3D printing’s integration within dental laboratories and practices. Its primary objective is to offer a systematic evaluation of the existing applications of 3D-printing technology within the realm of dental prosthetic restorations. Furthermore, this article delves into potential prospects, while also critically examining the sustained relevance of conventional dental laboratory services and manufacturing procedures. The central focus of this article is to expound upon the extent to which 3D printing is presently harnessed for crafting dental prosthetic appliances. By presenting verifiable data and factual insights, this article aspires to elucidate the actual implementation of 3D printing in prosthetic dentistry and its seamless integration into dental practices. The aim of this narrative review is twofold: firstly, to provide an informed and unbiased evaluation of the role that 3D printing currently plays within dental laboratories and practices; and secondly, to instigate contemplation on the transformative potential of this technology, both in terms of its contemporary impact and its future implications, while maintaining a balanced consideration of traditional dental approaches.
Improving the Nutritional Value and Physical Properties of Gluten-Free Mushroom Soup by Substituting Rice Flour with Quinoa Seed Flour
Authors: Saed Badr , El-Waseif Mohammed , Ali Hatem , Alsulami Tawfiq , Ban Zhaojun , Farouk Amr
Published: November 2023

Gluten-free products were initially for people with gluten-related disorders but are now popular with regular consumers. Research is investigating the health benefits of other gluten-free pseudo cereals, like quinoa, with higher nutritional value. This study aimed to explore the impact of substituting 30% rice flour (RF) in gluten-free mushroom instant dry soup with quinoa seed flour (QSF) at different levels on its nutritional, physicochemical, sensory characteristics, and the antioxidant activity. The results showed that replacing RF with QSF led to a significant improvement in the nutritional profile of the soup, with a linear increase in crude protein, fat, ash, crude fiber, total polyphenol, flavonoids, and antioxidant activity levels as the substitution rate increased. Moreover, macro- and microelements and essential amino acids increased significantly in the soup samples by adding QSF instead of RF, from 10 to 30%, compared to the control, which contained 30% of RF. However, the other total carbohydrate content decreased with the rise in the QSF concentration in the soup. In addition, substituting RF with QSF improved the soup samples’ rehydration ratio and total soluble solids. However, the color parameters (a* and b*) were increased with a decrease in L*. The sensory analysis revealed that the maximum substitution rate of QSF maintained the highest consumer acceptability, odor, flavor, texture, and appearance of the gluten-free mushroom instant soup samples. In conclusion, adding QSF to non-gluten soups improves their physical and chemical characteristics, nutritional impact, and organoleptic properties due to phytochemicals.
Analysis via 3D FEM of the Passing Capacity of Pipeline Inspection Gauges in Bends with Different Curvatures
Authors: Zhou Faqi , Lin Haoye , Zhang Ying , Zhao Suyang , Fu Shuangcheng , Zhang Hui
Published: November 2023

Pipeline inspection gauges easily become wedged in offshore and onshore small-diameter pipelines (where the outer diameter, D, of the pipe is less than 150 mm), particularly at the bends. To reveal the relationship between PIG capacity and bend curvature radius, a quantitative study on the passing capacity of PIG was conducted in this paper from three key perspectives of performance: safe application, sealing, and driving. The results demonstrate that the pipeline inspection gauge exhibits better passing capacity as the curvature radius of the bend increases. To improve the poorest passing capacity, in the case of R = 3D, different numbers of grooves are opened in the cup. The results demonstrate that the cup with 24 square grooves has a substantial impact on optimizing the passing capacity of the pipeline inspection gauge. This enhancement results in improvements in safe application performance (40.8%), sealing performance (12.22%), and driving performance (17%). This research aims to expand our understanding of blockages in small-diameter pipelines and provide a basis for optimizing the structure of the pipeline inspection gauge for small-diameter pipelines.
Study on the Medium-Temperature Reduction Behavior of Methane and Laterite Nickel Ore
Authors: Shi Ying , Qi Sheng , Liu Xingyang , Liu Shoujun , Yan Jinyao , Yang Song
Published: November 2023

Under the background of carbon peaking and carbon neutrality, compared with a traditional carbon thermal reduction to produce nickel, gas-based reduction has outstanding advantages in efficiency and carbon reduction. However, there are few studies on gas-based reduction at present. This article explores the reduction behavior of methane on laterite nickel ore. The effects of different reaction temperatures, reaction times and gas concentrations on the reduction reaction were studied. The phase and morphology of laterite nickel ore and its calcined products were analyzed by XRD, SEM-EDS and gas chromatography. The results show that when the reduction temperature is 900 °C, the reduction time is 60 min, and the concentration of CH4 is 50%, a concentrate with a nickel grade of 3.06% and a recovery of 52.09% can be obtained. After analysis, the reason for the low nickel grade and recovery rate in the concentrate is, on the one hand, due to the incomplete reduction of NiO, and a large amount of nickel still exists in the silicate in the tailings; on the other hand, it is due to the fine grain size of the partially reduced nickel iron alloy, which is lost in the tailings and filtrate.
Kierkegaard on Hope and Faith
Authors: Rudd Anthony
Published: November 2023

Faith, hope and love have often been classed together in the Christian tradition as the three “theological virtues”. Kierkegaard does not use that label for them, but he does have a good deal to say about all three. This paper starts by examining hope, arguing that there is an Aristotelian-style virtue relating to hope (a mean between wishful and depressive thinking) and that Kierkegaard could consistently recognize it as a secular virtue. However, his main discussions of hope as a positive state are in a religious context and relate it closely to faith and love; proper hope is a work of love and grounded in faith in God. I then argue that Kierkegaard’s understanding of faith, hope and love is, in many respects, close to Aquinas’ understanding of them as theological virtues (which differs in important ways from Aristotle’s account of a virtue) and that, therefore, it is appropriate to see Kierkegaard’s religious thought as lying within the tradition of virtue theory. The main difference between Aquinas and Kierkegaard here is that the former has an intellectualist and propositional account of faith which contrasts with the latter’s affective and existential view of it. This means that hope and love are both closer to faith for Kierkegaard than they are for Aquinas, meaning that he has a tight account of the unity of the theological virtues. I conclude by discussing how both faith and hope operate as antidotes to despair in The Sickness Unto Death.
A Sustainable Approach to Tourist Signage on Heritage Trails
Authors: Deri Maria Grazia , Chiti Letizia , Ciuffoletti Augusto
Published: November 2023

Understanding the cultural aspects of an area rich in heritage is crucial for building a lasting educational experience from an excursion. Many articles in the literature explore the use of sophisticated technologies to achieve such a goal. Tourism proposals for inland areas are significant because the presence of human artifacts and signage can harm the experience and create pollutants. Through a holistic methodology and analysis, this paper examines the signage for an area rich in cultural and natural assets: this study encompasses history, touristic vocation, and the environmental context. According to the analysis, slow, community-involved tourism is the preferred destination, and the signage solution must meet strict sustainability requirements in the social, economic, and environmental realms. After applying the appropriate governance guidelines, QR code technology was selected for a thoroughly documented experimental deployment.